doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_head	arg_protein	arg_domain	arg_site	arg_sugar	arg_head_offset	arg_base_np	arg_base_np_offset	arg_np	arg_np_offset	sent_text	PSource	NProtein	NID	SiteSource	SiteName
35257727	7	0	with	structure	1156:1164	arg1	biocompatibility					1234:1249	good biocompatibility	1229:1249	good biocompatibility	1229:1249	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	7	0	with	structure	1156:1164	arg1	effect					1217:1222	excellent shape memory effect	1194:1222	excellent shape memory effect	1194:1222	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	7	0	with	structure	1156:1164	arg1	distribution					1180:1191	gradient distribution	1171:1191	gradient distribution	1171:1191	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	9	1	contain	have	1445:1448	arg1	properties					1391:1400	These excellent properties	1375:1400	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels	1375:1443	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	9	1	contain	have	1445:1448	arg2	prospects					1469:1477	prospects	1469:1477	prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering	1469:1564	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	7	2	link	cross-linked	1095:1106	arg1	hydrogels					1108:1116	ionic cross-linked hydrogels	1089:1116	ionic cross-linked hydrogels	1089:1116	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	7	3	theme	porous	1149:1154	arg1	structure					1156:1164	a unique three-dimensional porous structure	1122:1164	a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility	1122:1249	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	7	4	theme	unique	1124:1129	arg1	structure					1156:1164	a unique three-dimensional porous structure	1122:1164	a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility	1122:1249	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	9	5	theme	electronic	1491:1500	arg1	devices					1502:1508	flexible electronic devices	1482:1508	flexible electronic devices	1482:1508	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	8	6	theme	Ca2+	1274:1277	arg1	hydrogels					1292:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels	1252:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels	1252:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels were nontoxic and conducive to the adhesion and growth of Schwann cells.					
35257727	7	7	theme	cross-linked	1095:1106	arg1	hydrogels					1108:1116	ionic cross-linked hydrogels	1089:1116	ionic cross-linked hydrogels	1089:1116	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	1	8	theme	bond-ion	255:262	arg1	structure					301:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	7	9	theme	shape	1204:1208	arg1	effect					1217:1222	excellent shape memory effect	1194:1222	excellent shape memory effect	1194:1222	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	1	10	theme	bond	281:284	arg1	structure					301:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	4	11	theme	Fe3+	726:729	arg1	hydrogels					744:752	Fe3+ cross-linked hydrogels	726:752	Fe3+ cross-linked hydrogels	726:752	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	6	12	used	used	999:1002	arg2	pen					1020:1022	a capacitive pen	1007:1022	a capacitive pen in mobile phone writing, painting and dialing numbers	1007:1076	Interestingly, the hydrogel can be used as a capacitive pen in mobile phone writing, painting and dialing numbers.					
35257727	6	12	used	used	999:1002	arg2	hydrogel					983:990	the hydrogel	979:990	the hydrogel	979:990	Interestingly, the hydrogel can be used as a capacitive pen in mobile phone writing, painting and dialing numbers.					
35257727	2	13	theme	hydrogels	559:567	arg1	properties					531:540	properties	531:540	properties	531:540	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	13	theme	hydrogels	559:567	arg1	structure					504:512	structure	504:512	structure	504:512	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	13	theme	hydrogels	559:567	arg1	morphology					515:524	morphology	515:524	morphology	515:524	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	4	14	theme	hydrogels	744:752	arg1	strength					714:721	The compressive strength	698:721	The compressive strength of Fe3+ cross-linked hydrogels	698:752	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	4	14	theme	hydrogels	744:752	arg1	times					774:778	5.56 MPa, 16.13 times	758:778	times	774:778	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	9	15	theme	tissue	1547:1552	arg1	engineering					1554:1564	tissue engineering	1547:1564	tissue engineering	1547:1564	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	9	16	theme	electronic	1525:1534	arg1	skins					1536:1540	soft electronic skins	1520:1540	soft electronic skins	1520:1540	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	0	17	theme	sodium	69:74	arg1	protein/polyacrylamide-Strength					91:121	sodium alginate/krill protein/polyacrylamide-Strength	69:121	sodium alginate/krill protein/polyacrylamide-Strength	69:121	Preparation and characterization of multi-network hydrogels based on sodium alginate/krill protein/polyacrylamide-Strength, shape memory, conductivity and biocompatibility.					
35257727	7	18	theme	memory	1210:1215	arg1	effect					1217:1222	excellent shape memory effect	1194:1222	excellent shape memory effect	1194:1222	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	1	19	theme	protein/polyacrylamide	195:216	arg1	hydrogel					231:238	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel	173:238	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure	173:309	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	6	20	theme	phone	1034:1038	arg1	writing					1040:1046	mobile phone writing	1027:1046	mobile phone writing	1027:1046	Interestingly, the hydrogel can be used as a capacitive pen in mobile phone writing, painting and dialing numbers.					
35257727	4	21	dep	times	774:778	arg1	that					780:783	that	780:783	that	780:783	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	8	22	theme	cells	1368:1372	arg1	growth					1350:1355	growth	1350:1355	growth	1350:1355	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels were nontoxic and conducive to the adhesion and growth of Schwann cells.					
35257727	8	22	theme	cells	1368:1372	arg1	adhesion					1337:1344	adhesion	1337:1344	adhesion	1337:1344	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels were nontoxic and conducive to the adhesion and growth of Schwann cells.					
35257727	0	23	theme	shape	124:128	arg1	memory					130:135	shape memory	124:135	shape memory	124:135	Preparation and characterization of multi-network hydrogels based on sodium alginate/krill protein/polyacrylamide-Strength, shape memory, conductivity and biocompatibility.					
35257727	6	24	theme	capacitive	1009:1018	arg1	hydrogel					983:990	the hydrogel	979:990	the hydrogel	979:990	Interestingly, the hydrogel can be used as a capacitive pen in mobile phone writing, painting and dialing numbers.					
35257727	6	24	theme	capacitive	1009:1018	arg1	pen					1020:1022	a capacitive pen	1007:1022	a capacitive pen in mobile phone writing, painting and dialing numbers	1007:1076	Interestingly, the hydrogel can be used as a capacitive pen in mobile phone writing, painting and dialing numbers.					
35257727	6	25	from	pen	1020:1022	arg1	numbers					1070:1076	dialing numbers	1062:1076	dialing numbers	1062:1076	Interestingly, the hydrogel can be used as a capacitive pen in mobile phone writing, painting and dialing numbers.					
35257727	6	25	from	pen	1020:1022	arg1	writing					1040:1046	mobile phone writing	1027:1046	mobile phone writing	1027:1046	Interestingly, the hydrogel can be used as a capacitive pen in mobile phone writing, painting and dialing numbers.					
35257727	6	25	from	pen	1020:1022	arg1	painting					1049:1056	painting	1049:1056	painting	1049:1056	Interestingly, the hydrogel can be used as a capacitive pen in mobile phone writing, painting and dialing numbers.					
35257727	5	26	contain	had	890:892	arg2	conductivity					912:923	significant ionic conductivity	894:923	significant ionic conductivity	894:923	The results of ionic conductivity measurements showed that hydrogels had significant ionic conductivity and were sensitive to external forces.					
35257727	5	26	contain	had	890:892	arg1	hydrogels					880:888	hydrogels	880:888	hydrogels	880:888	The results of ionic conductivity measurements showed that hydrogels had significant ionic conductivity and were sensitive to external forces.					
35257727	4	27	theme	non-ionic	788:796	arg1	hydrogels					810:818	non-ionic crosslinked hydrogels	788:818	non-ionic crosslinked hydrogels	788:818	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	7	28	theme	good	1229:1232	arg1	biocompatibility					1234:1249	good biocompatibility	1229:1249	good biocompatibility	1229:1249	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	1	29	theme	Sodium	173:178	arg1	hydrogel					231:238	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel	173:238	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure	173:309	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	4	30	theme	5.56 MPa	758:765	arg1	strength					714:721	The compressive strength	698:721	The compressive strength of Fe3+ cross-linked hydrogels	698:752	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	4	30	theme	5.56 MPa	758:765	arg1	times					774:778	5.56 MPa, 16.13 times	758:778	times	774:778	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	5	31	theme	conductivity	842:853	arg1	measurements					855:866	ionic conductivity measurements	836:866	ionic conductivity measurements	836:866	The results of ionic conductivity measurements showed that hydrogels had significant ionic conductivity and were sensitive to external forces.					
35257727	1	32	theme	ion	365:367	arg1	crosslinking					369:380	complexion ion crosslinking	354:380	complexion ion crosslinking	354:380	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	3	33	theme	ionic	644:648	arg1	hydrogels					663:671	ionic cross-linked hydrogels	644:671	ionic cross-linked hydrogels	644:671	The results showed that the mechanical strength of ionic cross-linked hydrogels increased significantly.					
35257727	2	34	theme	ion	450:452	arg1	Fe3+					463:466	Fe3+	463:466	Fe3+	463:466	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	34	theme	ion	450:452	arg1	Ba2+					469:472	Ba2+	469:472	Ba2+	469:472	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	34	theme	ion	450:452	arg1	Zn2+					491:494	Zn2+	491:494	Zn2+	491:494	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	34	theme	ion	450:452	arg1	species					454:460	ion species	450:460	ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+)	450:495	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	34	theme	ion	450:452	arg1	Sr2+					475:478	Sr2+	475:478	Sr2+	475:478	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	34	theme	ion	450:452	arg1	Ca2+					481:484	Ca2+	481:484	Ca2+	481:484	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	0	35	theme	multi-network	36:48	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of multi-network hydrogels based on sodium alginate/krill protein/polyacrylamide-Strength, shape memory, conductivity and biocompatibility.					
35257727	0	35	theme	multi-network	36:48	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of multi-network hydrogels based on sodium alginate/krill protein/polyacrylamide-Strength, shape memory, conductivity and biocompatibility.					
35257727	9	36	theme	excellent	1381:1389	arg1	properties					1391:1400	These excellent properties	1375:1400	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels	1375:1443	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	7	37	theme	excellent	1194:1202	arg1	effect					1217:1222	excellent shape memory effect	1194:1222	excellent shape memory effect	1194:1222	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	4	38	link	crosslinked	798:808	arg1	hydrogels					810:818	non-ionic crosslinked hydrogels	788:818	non-ionic crosslinked hydrogels	788:818	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	8	39	dep	adhesion	1337:1344	arg1	the					1333:1335	the	1333:1335	the	1333:1335	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels were nontoxic and conducive to the adhesion and growth of Schwann cells.					
35257727	2	40	from	effects	439:445	arg1	properties					531:540	properties	531:540	properties	531:540	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	40	from	effects	439:445	arg1	structure					504:512	structure	504:512	structure	504:512	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	40	from	effects	439:445	arg1	morphology					515:524	morphology	515:524	morphology	515:524	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	3	41	link	cross-linked	650:661	arg1	hydrogels					663:671	ionic cross-linked hydrogels	644:671	ionic cross-linked hydrogels	644:671	The results showed that the mechanical strength of ionic cross-linked hydrogels increased significantly.					
35257727	4	42	theme	hydrogels	810:818	arg1	strength					714:721	The compressive strength	698:721	The compressive strength of Fe3+ cross-linked hydrogels	698:752	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	4	42	theme	hydrogels	810:818	arg1	times					774:778	5.56 MPa, 16.13 times	758:778	times	774:778	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	1	43	theme	"	285:285	arg1	structure					301:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	1	44	with	hydrogel	231:238	arg1	structure					301:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	3	45	theme	hydrogels	663:671	arg1	strength					632:639	the mechanical strength	617:639	the mechanical strength of ionic cross-linked hydrogels	617:671	The results showed that the mechanical strength of ionic cross-linked hydrogels increased significantly.					
35257727	5	46	theme	significant	894:904	arg1	conductivity					912:923	significant ionic conductivity	894:923	significant ionic conductivity	894:923	The results of ionic conductivity measurements showed that hydrogels had significant ionic conductivity and were sensitive to external forces.					
35257727	9	47	theme	hydrogels	1435:1443	arg1	properties					1391:1400	These excellent properties	1375:1400	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels	1375:1443	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	9	48	theme	broad	1450:1454	arg1	applications					1456:1467	broad applications	1450:1467	broad applications	1450:1467	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	9	49	from	prospects	1469:1477	arg1	devices					1502:1508	flexible electronic devices	1482:1508	flexible electronic devices	1482:1508	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	9	49	from	prospects	1469:1477	arg1	skins					1536:1540	soft electronic skins	1520:1540	soft electronic skins	1520:1540	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	9	49	from	prospects	1469:1477	arg1	engineering					1554:1564	tissue engineering	1547:1564	tissue engineering	1547:1564	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	9	49	from	prospects	1469:1477	arg1	sensors					1511:1517	sensors	1511:1517	sensors	1511:1517	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	7	50	theme	three-dimensional	1131:1147	arg1	structure					1156:1164	a unique three-dimensional porous structure	1122:1164	a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility	1122:1249	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	1	51	theme	covalent	327:334	arg1	cross-linking					336:348	covalent cross-linking	327:348	covalent cross-linking	327:348	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	4	52	link	cross-linked	731:742	arg1	hydrogels					744:752	Fe3+ cross-linked hydrogels	726:752	Fe3+ cross-linked hydrogels	726:752	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	9	53	theme	flexible	1482:1489	arg1	devices					1502:1508	flexible electronic devices	1482:1508	flexible electronic devices	1482:1508	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	8	54	theme	Sr2+	1264:1267	arg1	hydrogels					1292:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels	1252:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels	1252:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels were nontoxic and conducive to the adhesion and growth of Schwann cells.					
35257727	5	55	theme	ionic	906:910	arg1	conductivity					912:923	significant ionic conductivity	894:923	significant ionic conductivity	894:923	The results of ionic conductivity measurements showed that hydrogels had significant ionic conductivity and were sensitive to external forces.					
35257727	9	56	link	cross-linked	1411:1422	arg1	hydrogels					1435:1443	ionic cross-linked SA/AKP/PAM hydrogels	1405:1443	ionic cross-linked SA/AKP/PAM hydrogels	1405:1443	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	7	57	theme	ionic	1089:1093	arg1	hydrogels					1108:1116	ionic cross-linked hydrogels	1089:1116	ionic cross-linked hydrogels	1089:1116	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	1	58	theme	covalent	246:253	arg1	structure					301:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	1	59	theme	SA/AKP/PAM	219:228	arg1	hydrogel					231:238	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel	173:238	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure	173:309	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	8	60	theme	cross-linked	1279:1290	arg1	hydrogels					1292:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels	1252:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels	1252:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels were nontoxic and conducive to the adhesion and growth of Schwann cells.					
35257727	9	61	theme	soft	1520:1523	arg1	skins					1536:1540	soft electronic skins	1520:1540	soft electronic skins	1520:1540	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	8	62	theme	Fe3+	1252:1255	arg1	hydrogels					1292:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels	1252:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels	1252:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels were nontoxic and conducive to the adhesion and growth of Schwann cells.					
35257727	5	63	theme	external	947:954	arg1	forces					956:961	external forces	947:961	external forces	947:961	The results of ionic conductivity measurements showed that hydrogels had significant ionic conductivity and were sensitive to external forces.					
35257727	8	64	theme	Ba2+	1258:1261	arg1	hydrogels					1292:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels	1252:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels	1252:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels were nontoxic and conducive to the adhesion and growth of Schwann cells.					
35257727	7	65	contain	had	1118:1120	arg1	hydrogels					1108:1116	ionic cross-linked hydrogels	1089:1116	ionic cross-linked hydrogels	1089:1116	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	7	65	contain	had	1118:1120	arg2	structure					1156:1164	a unique three-dimensional porous structure	1122:1164	a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility	1122:1249	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	2	66	theme	multi-network	545:557	arg1	hydrogels					559:567	multi-network hydrogels	545:567	multi-network hydrogels	545:567	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	4	67	theme	cross-linked	731:742	arg1	hydrogels					744:752	Fe3+ cross-linked hydrogels	726:752	Fe3+ cross-linked hydrogels	726:752	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	0	68	theme	alginate/krill	76:89	arg1	protein/polyacrylamide-Strength					91:121	sodium alginate/krill protein/polyacrylamide-Strength	69:121	sodium alginate/krill protein/polyacrylamide-Strength	69:121	Preparation and characterization of multi-network hydrogels based on sodium alginate/krill protein/polyacrylamide-Strength, shape memory, conductivity and biocompatibility.					
35257727	5	69	theme	measurements	855:866	arg1	results					825:831	The results	821:831	The results of ionic conductivity measurements	821:866	The results of ionic conductivity measurements showed that hydrogels had significant ionic conductivity and were sensitive to external forces.					
35257727	1	70	theme	alginate/krill	180:193	arg1	hydrogel					231:238	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel	173:238	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure	173:309	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	9	71	theme	cross-linked	1411:1422	arg1	hydrogels					1435:1443	ionic cross-linked SA/AKP/PAM hydrogels	1405:1443	ionic cross-linked SA/AKP/PAM hydrogels	1405:1443	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	6	72	theme	mobile	1027:1032	arg1	writing					1040:1046	mobile phone writing	1027:1046	mobile phone writing	1027:1046	Interestingly, the hydrogel can be used as a capacitive pen in mobile phone writing, painting and dialing numbers.					
35257727	4	73	theme	compressive	702:712	arg1	strength					714:721	The compressive strength	698:721	The compressive strength of Fe3+ cross-linked hydrogels	698:752	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	4	73	theme	compressive	702:712	arg1	times					774:778	5.56 MPa, 16.13 times	758:778	times	774:778	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	8	74	theme	Schwann	1360:1366	arg1	cells					1368:1372	Schwann cells	1360:1372	Schwann cells	1360:1372	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels were nontoxic and conducive to the adhesion and growth of Schwann cells.					
35257727	1	75	theme	multi-network	287:299	arg1	structure					301:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	1	76	theme	raw	420:422	arg1	acrylamide					401:410	acrylamide	401:410	acrylamide (AM)	401:415	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	1	76	theme	raw	420:422	arg1	SA					388:389	SA	388:389	SA	388:389	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	1	76	theme	raw	420:422	arg1	materials					424:432	raw materials	420:432	raw materials	420:432	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	1	76	theme	raw	420:422	arg1	AKP					392:394	AKP	392:394	AKP	392:394	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	5	77	theme	ionic	836:840	arg1	measurements					855:866	ionic conductivity measurements	836:866	ionic conductivity measurements	836:866	The results of ionic conductivity measurements showed that hydrogels had significant ionic conductivity and were sensitive to external forces.					
35257727	1	78	theme	complexion	354:363	arg1	crosslinking					369:380	complexion ion crosslinking	354:380	complexion ion crosslinking	354:380	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	2	79	dep	structure	504:512	arg1	the					500:502	the	500:502	the	500:502	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	80	theme	species	454:460	arg1	effects					439:445	The effects	435:445	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels	435:567	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	1	81	theme	complex-hydrogen	264:279	arg1	structure					301:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	"covalent bond-ion complex-hydrogen bond" multi-network structure	245:309	Sodium alginate/krill protein/polyacrylamide (SA/AKP/PAM) hydrogel with "covalent bond-ion complex-hydrogen bond" multi-network structure was prepared by covalent cross-linking and complexion ion crosslinking using SA, AKP, and acrylamide (AM) as raw materials.					
35257727	6	82	theme	dialing	1062:1068	arg1	numbers					1070:1076	dialing numbers	1062:1076	dialing numbers	1062:1076	Interestingly, the hydrogel can be used as a capacitive pen in mobile phone writing, painting and dialing numbers.					
35257727	3	83	theme	mechanical	621:630	arg1	strength					632:639	the mechanical strength	617:639	the mechanical strength of ionic cross-linked hydrogels	617:671	The results showed that the mechanical strength of ionic cross-linked hydrogels increased significantly.					
35257727	7	84	theme	gradient	1171:1178	arg1	distribution					1180:1191	gradient distribution	1171:1191	gradient distribution	1171:1191	Moreover, ionic cross-linked hydrogels had a unique three-dimensional porous structure with gradient distribution, excellent shape memory effect, and good biocompatibility.					
35257727	0	85	dep	hydrogels	50:58	arg1	based					60:64	based	60:64	hydrogels based on sodium alginate/krill protein/polyacrylamide-Strength, shape memory, conductivity and biocompatibility	50:170	Preparation and characterization of multi-network hydrogels based on sodium alginate/krill protein/polyacrylamide-Strength, shape memory, conductivity and biocompatibility.					
35257727	2	86	dep	species	454:460	arg1	Fe3+					463:466	Fe3+	463:466	Fe3+	463:466	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	86	dep	species	454:460	arg1	Ba2+					469:472	Ba2+	469:472	Ba2+	469:472	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	86	dep	species	454:460	arg1	Zn2+					491:494	Zn2+	491:494	Zn2+	491:494	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	86	dep	species	454:460	arg1	species					454:460	ion species	450:460	ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+)	450:495	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	86	dep	species	454:460	arg1	Sr2+					475:478	Sr2+	475:478	Sr2+	475:478	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	2	86	dep	species	454:460	arg1	Ca2+					481:484	Ca2+	481:484	Ca2+	481:484	The effects of ion species (Fe3+, Ba2+, Sr2+, Ca2+, and Zn2+) on the structure, morphology, and properties of multi-network hydrogels were studied in detail.					
35257727	9	87	theme	ionic	1405:1409	arg1	hydrogels					1435:1443	ionic cross-linked SA/AKP/PAM hydrogels	1405:1443	ionic cross-linked SA/AKP/PAM hydrogels	1405:1443	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	3	88	theme	cross-linked	650:661	arg1	hydrogels					663:671	ionic cross-linked hydrogels	644:671	ionic cross-linked hydrogels	644:671	The results showed that the mechanical strength of ionic cross-linked hydrogels increased significantly.					
35257727	4	89	theme	crosslinked	798:808	arg1	hydrogels					810:818	non-ionic crosslinked hydrogels	788:818	non-ionic crosslinked hydrogels	788:818	The compressive strength of Fe3+ cross-linked hydrogels was 5.56 MPa, 16.13 times that of non-ionic crosslinked hydrogels.					
35257727	9	90	theme	SA/AKP/PAM	1424:1433	arg1	hydrogels					1435:1443	ionic cross-linked SA/AKP/PAM hydrogels	1405:1443	ionic cross-linked SA/AKP/PAM hydrogels	1405:1443	These excellent properties of ionic cross-linked SA/AKP/PAM hydrogels have broad applications prospects in flexible electronic devices, sensors, soft electronic skins, and tissue engineering.					
35257727	8	91	link	cross-linked	1279:1290	arg1	hydrogels					1292:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels	1252:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels	1252:1300	Fe3+, Ba2+, Sr2+, and Ca2+ cross-linked hydrogels were nontoxic and conducive to the adhesion and growth of Schwann cells.					
36529359	8	0	dep	term	1117:1120	arg1	long					1112:1115	long	1112:1115	long	1112:1115	The patches were found to be stable for desired characteristics for up to 3 months in long term storage conditions.					
36529359	11	1	theme	athymic	1561:1567	arg1	mice					1574:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	11	2	theme	gastric	1508:1514	arg1	patch					1516:1520	regorafenib-loaded gastric patch	1489:1520	regorafenib-loaded gastric patch	1489:1520	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	2	3	theme	entrapped	302:310	arg1	agent					329:333	entrapped chemotherapeutic agent	302:333	entrapped chemotherapeutic agent for eight days after oral administration	302:374	Here, we report development of a multi-layered mucoadhesive gastric patch that could deliver entrapped chemotherapeutic agent for eight days after oral administration.					
36529359	8	4	dep	3	1100:1100	arg1	to					1097:1098	to	1097:1098	to	1097:1098	The patches were found to be stable for desired characteristics for up to 3 months in long term storage conditions.					
36529359	9	5	theme	gastric	1287:1293	arg1	patch					1295:1299	gastric patch	1287:1299	gastric patch	1287:1299	The pharmacokinetic studies in rat model revealed constant plasma concentration of regorafenib sustained for 8 days after oral administration of gastric patch.					
36529359	5	6	theme	rat	691:693	arg1	mucosa					703:708	freshly excised rat gastric mucosa	675:708	freshly excised rat gastric mucosa	675:708	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate showed greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa.					
36529359	3	7	contain	contain	417:423	arg2	layer					450:454	mucoadhesive layer	437:454	mucoadhesive layer	437:454	The multi-layered patch was designed to contain core layer, mucoadhesive layer and backing layer.					
36529359	3	7	contain	contain	417:423	arg1	patch					395:399	The multi-layered patch	377:399	The multi-layered patch	377:399	The multi-layered patch was designed to contain core layer, mucoadhesive layer and backing layer.					
36529359	3	7	contain	contain	417:423	arg2	layer					430:434	core layer	425:434	core layer	425:434	The multi-layered patch was designed to contain core layer, mucoadhesive layer and backing layer.					
36529359	3	7	contain	contain	417:423	arg2	layer					468:472	backing layer	460:472	backing layer	460:472	The multi-layered patch was designed to contain core layer, mucoadhesive layer and backing layer.					
36529359	11	8	theme	bearing	1553:1559	arg1	mice					1574:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	11	9	theme	tumor	1547:1551	arg1	mice					1574:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	5	10	theme	chitosan-hydrocaffeic	578:598	arg1	conjugate					605:613	chitosan-hydrocaffeic acid conjugate	578:613	chitosan-hydrocaffeic acid conjugate	578:613	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate showed greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa.					
36529359	12	11	theme	drug	1783:1786	arg1	patch					1738:1742	the multi-layered mucoadhesive gastric patch	1699:1742	the multi-layered mucoadhesive gastric patch	1699:1742	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	12	11	theme	drug	1783:1786	arg1	system					1797:1802	a long-acting oral drug delivery system	1764:1802	a long-acting oral drug delivery system	1764:1802	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	11	12	theme	tumor	1627:1631	arg1	volume					1633:1638	the tumor volume	1623:1638	the tumor volume over 7 days	1623:1650	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	2	13	theme	patch	277:281	arg1	development					225:235	development	225:235	development of a multi-layered mucoadhesive gastric patch that could deliver entrapped chemotherapeutic agent for eight days after oral administration	225:374	Here, we report development of a multi-layered mucoadhesive gastric patch that could deliver entrapped chemotherapeutic agent for eight days after oral administration.					
36529359	5	14	theme	mucoadhesive	551:562	arg1	layer					564:568	The mucoadhesive layer	547:568	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate	547:613	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate showed greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa.					
36529359	1	15	theme	long-acting	149:159	arg1	form					188:191	a long-acting orally administered dosage form	147:191	a long-acting orally administered dosage form	147:191	The development of a long-acting orally administered dosage form is a challenge.					
36529359	2	16	dep	deliver	294:300	arg1	could					288:292	could	288:292	could	288:292	Here, we report development of a multi-layered mucoadhesive gastric patch that could deliver entrapped chemotherapeutic agent for eight days after oral administration.					
36529359	10	17	theme	gastric	1306:1312	arg1	tissue					1314:1319	The gastric tissue	1302:1319	The gastric tissue where the patch adhered for 8 days	1302:1354	The gastric tissue where the patch adhered for 8 days did not show any significant histological changes compared with the normal gastric tissue.					
36529359	0	18	theme	mucoadhesive	73:84	arg1	delivery					86:93	long-acting mucoadhesive delivery	61:93	long-acting mucoadhesive delivery of model chemotherapeutic agent	61:125	Hydrocaffeic acid-chitosan coating of gastric patch provides long-acting mucoadhesive delivery of model chemotherapeutic agent.					
36529359	2	19	theme	mucoadhesive	256:267	arg1	patch					277:281	a multi-layered mucoadhesive gastric patch	240:281	a multi-layered mucoadhesive gastric patch that could deliver entrapped chemotherapeutic agent for eight days after oral administration	240:374	Here, we report development of a multi-layered mucoadhesive gastric patch that could deliver entrapped chemotherapeutic agent for eight days after oral administration.					
36529359	1	20	theme	administered	168:179	arg1	form					188:191	a long-acting orally administered dosage form	147:191	a long-acting orally administered dosage form	147:191	The development of a long-acting orally administered dosage form is a challenge.					
36529359	5	21	theme	18.1	656:659	arg1	±					661:661	±	661:661	±	661:661	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate showed greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa.					
36529359	1	22	theme	form	188:191	arg1	challenge					198:206	a challenge	196:206	a challenge	196:206	The development of a long-acting orally administered dosage form is a challenge.					
36529359	1	22	theme	form	188:191	arg1	development					132:142	The development	128:142	The development of a long-acting orally administered dosage form	128:191	The development of a long-acting orally administered dosage form is a challenge.					
36529359	12	23	theme	long-acting	1766:1776	arg1	patch					1738:1742	the multi-layered mucoadhesive gastric patch	1699:1742	the multi-layered mucoadhesive gastric patch	1699:1742	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	12	23	theme	long-acting	1766:1776	arg1	system					1797:1802	a long-acting oral drug delivery system	1764:1802	a long-acting oral drug delivery system	1764:1802	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	0	24	theme	chemotherapeutic	104:119	arg1	agent					121:125	model chemotherapeutic agent	98:125	model chemotherapeutic agent	98:125	Hydrocaffeic acid-chitosan coating of gastric patch provides long-acting mucoadhesive delivery of model chemotherapeutic agent.					
36529359	5	25	theme	0.78	663:666	arg1	±					661:661	±	661:661	±	661:661	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate showed greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa.					
36529359	11	26	theme	xenografted	1535:1545	arg1	mice					1574:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	11	27	theme	cell	1530:1533	arg1	mice					1574:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	8	28	theme	term	1117:1120	arg1	conditions					1130:1139	long term storage conditions	1112:1139	long term storage conditions	1112:1139	The patches were found to be stable for desired characteristics for up to 3 months in long term storage conditions.					
36529359	5	29	theme	greatest	622:629	arg1	strength					644:651	greatest mucoadhesion strength	622:651	greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa	622:708	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate showed greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa.					
36529359	4	30	theme	chemotherapeutic	510:525	arg1	regorafenib					534:544	regorafenib	534:544	regorafenib	534:544	The core layer contained the model chemotherapeutic agent, regorafenib.					
36529359	4	30	theme	chemotherapeutic	510:525	arg1	agent					527:531	the model chemotherapeutic agent	500:531	the model chemotherapeutic agent	500:531	The core layer contained the model chemotherapeutic agent, regorafenib.					
36529359	6	31	theme	hydrophobic	737:747	arg1	composite					787:795	hydrophobic polycaprolactone-polydimethylsiloxane composite	737:795	hydrophobic polycaprolactone-polydimethylsiloxane composite	737:795	The backing layer made of hydrophobic polycaprolactone-polydimethylsiloxane composite showed the contact angle of 120 ± 4.7° after placement of water drop.					
36529359	0	32	theme	Hydrocaffeic	0:11	arg1	coating					27:33	Hydrocaffeic acid-chitosan coating	0:33	Hydrocaffeic acid-chitosan coating of gastric patch	0:50	Hydrocaffeic acid-chitosan coating of gastric patch provides long-acting mucoadhesive delivery of model chemotherapeutic agent.					
36529359	7	33	theme	entrapped	871:879	arg1	regorafenib					881:891	The entrapped regorafenib	867:891	The entrapped regorafenib	867:891	The entrapped regorafenib predominantly released from the mucoadhesive-side of the patch into simulated gastric fluid and showed a zero-order release profile.					
36529359	7	34	theme	release	1009:1015	arg1	profile					1017:1023	a zero-order release profile	996:1023	a zero-order release profile	996:1023	The entrapped regorafenib predominantly released from the mucoadhesive-side of the patch into simulated gastric fluid and showed a zero-order release profile.					
36529359	10	35	theme	histological	1385:1396	arg1	changes					1398:1404	any significant histological changes	1369:1404	any significant histological changes	1369:1404	The gastric tissue where the patch adhered for 8 days did not show any significant histological changes compared with the normal gastric tissue.					
36529359	6	36	theme	backing	715:721	arg1	layer					723:727	The backing layer	711:727	The backing layer made of hydrophobic polycaprolactone-polydimethylsiloxane composite	711:795	The backing layer made of hydrophobic polycaprolactone-polydimethylsiloxane composite showed the contact angle of 120 ± 4.7° after placement of water drop.					
36529359	12	37	theme	mucoadhesive	1717:1728	arg1	patch					1738:1742	the multi-layered mucoadhesive gastric patch	1699:1742	the multi-layered mucoadhesive gastric patch	1699:1742	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	12	37	theme	mucoadhesive	1717:1728	arg1	system					1797:1802	a long-acting oral drug delivery system	1764:1802	a long-acting oral drug delivery system	1764:1802	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	3	38	theme	mucoadhesive	437:448	arg1	layer					430:434	core layer	425:434	core layer	425:434	The multi-layered patch was designed to contain core layer, mucoadhesive layer and backing layer.					
36529359	3	38	theme	mucoadhesive	437:448	arg1	layer					450:454	mucoadhesive layer	437:454	mucoadhesive layer	437:454	The multi-layered patch was designed to contain core layer, mucoadhesive layer and backing layer.					
36529359	9	39	theme	constant	1192:1199	arg1	concentration					1208:1220	constant plasma concentration	1192:1220	constant plasma concentration of regorafenib sustained for 8 days after oral administration of gastric patch	1192:1299	The pharmacokinetic studies in rat model revealed constant plasma concentration of regorafenib sustained for 8 days after oral administration of gastric patch.					
36529359	0	40	theme	gastric	38:44	arg1	patch					46:50	gastric patch	38:50	gastric patch	38:50	Hydrocaffeic acid-chitosan coating of gastric patch provides long-acting mucoadhesive delivery of model chemotherapeutic agent.					
36529359	11	41	theme	control	1668:1674	arg1	group					1676:1680	the control group	1664:1680	the control group	1664:1680	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	6	42	theme	drop	861:864	arg1	placement					842:850	placement	842:850	placement of water drop	842:864	The backing layer made of hydrophobic polycaprolactone-polydimethylsiloxane composite showed the contact angle of 120 ± 4.7° after placement of water drop.					
36529359	9	43	from	studies	1162:1168	arg1	model					1177:1181	rat model	1173:1181	rat model	1173:1181	The pharmacokinetic studies in rat model revealed constant plasma concentration of regorafenib sustained for 8 days after oral administration of gastric patch.					
36529359	9	44	theme	regorafenib	1225:1235	arg1	concentration					1208:1220	constant plasma concentration	1192:1220	constant plasma concentration of regorafenib sustained for 8 days after oral administration of gastric patch	1192:1299	The pharmacokinetic studies in rat model revealed constant plasma concentration of regorafenib sustained for 8 days after oral administration of gastric patch.					
36529359	2	45	theme	oral	356:359	arg1	administration					361:374	oral administration	356:374	oral administration	356:374	Here, we report development of a multi-layered mucoadhesive gastric patch that could deliver entrapped chemotherapeutic agent for eight days after oral administration.					
36529359	7	46	theme	zero-order	998:1007	arg1	profile					1017:1023	a zero-order release profile	996:1023	a zero-order release profile	996:1023	The entrapped regorafenib predominantly released from the mucoadhesive-side of the patch into simulated gastric fluid and showed a zero-order release profile.					
36529359	10	47	theme	normal	1424:1429	arg1	tissue					1439:1444	the normal gastric tissue	1420:1444	the normal gastric tissue	1420:1444	The gastric tissue where the patch adhered for 8 days did not show any significant histological changes compared with the normal gastric tissue.					
36529359	11	48	theme	oral	1451:1454	arg1	administration					1456:1469	The oral administration	1447:1469	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice	1447:1577	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	5	49	theme	gastric	695:701	arg1	mucosa					703:708	freshly excised rat gastric mucosa	675:708	freshly excised rat gastric mucosa	675:708	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate showed greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa.					
36529359	10	50	theme	gastric	1431:1437	arg1	tissue					1439:1444	the normal gastric tissue	1420:1444	the normal gastric tissue	1420:1444	The gastric tissue where the patch adhered for 8 days did not show any significant histological changes compared with the normal gastric tissue.					
36529359	11	51	theme	regorafenib-loaded	1489:1506	arg1	patch					1516:1520	regorafenib-loaded gastric patch	1489:1520	regorafenib-loaded gastric patch	1489:1520	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	9	52	theme	patch	1295:1299	arg1	administration					1269:1282	oral administration	1264:1282	oral administration of gastric patch	1264:1299	The pharmacokinetic studies in rat model revealed constant plasma concentration of regorafenib sustained for 8 days after oral administration of gastric patch.					
36529359	9	53	theme	oral	1264:1267	arg1	administration					1269:1282	oral administration	1264:1282	oral administration of gastric patch	1264:1299	The pharmacokinetic studies in rat model revealed constant plasma concentration of regorafenib sustained for 8 days after oral administration of gastric patch.					
36529359	2	54	theme	chemotherapeutic	312:327	arg1	agent					329:333	entrapped chemotherapeutic agent	302:333	entrapped chemotherapeutic agent for eight days after oral administration	302:374	Here, we report development of a multi-layered mucoadhesive gastric patch that could deliver entrapped chemotherapeutic agent for eight days after oral administration.					
36529359	7	55	theme	simulated	961:969	arg1	fluid					979:983	simulated gastric fluid	961:983	simulated gastric fluid	961:983	The entrapped regorafenib predominantly released from the mucoadhesive-side of the patch into simulated gastric fluid and showed a zero-order release profile.					
36529359	8	56	theme	desired	1066:1072	arg1	characteristics					1074:1088	desired characteristics	1066:1088	desired characteristics for up to 3 months in long term storage conditions	1066:1139	The patches were found to be stable for desired characteristics for up to 3 months in long term storage conditions.					
36529359	11	57	theme	dose	1481:1484	arg1	administration					1456:1469	The oral administration	1447:1469	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice	1447:1577	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	7	58	theme	patch	950:954	arg1	mucoadhesive-side					925:941	the mucoadhesive-side	921:941	the mucoadhesive-side of the patch	921:954	The entrapped regorafenib predominantly released from the mucoadhesive-side of the patch into simulated gastric fluid and showed a zero-order release profile.					
36529359	5	59	theme	excised	683:689	arg1	mucosa					703:708	freshly excised rat gastric mucosa	675:708	freshly excised rat gastric mucosa	675:708	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate showed greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa.					
36529359	6	60	theme	water	855:859	arg1	drop					861:864	water drop	855:864	water drop	855:864	The backing layer made of hydrophobic polycaprolactone-polydimethylsiloxane composite showed the contact angle of 120 ± 4.7° after placement of water drop.					
36529359	8	61	from	months	1102:1107	arg1	conditions					1130:1139	long term storage conditions	1112:1139	long term storage conditions	1112:1139	The patches were found to be stable for desired characteristics for up to 3 months in long term storage conditions.					
36529359	4	62	theme	core	479:482	arg1	layer					484:488	The core layer	475:488	The core layer	475:488	The core layer contained the model chemotherapeutic agent, regorafenib.					
36529359	2	63	theme	multi-layered	242:254	arg1	patch					277:281	a multi-layered mucoadhesive gastric patch	240:281	a multi-layered mucoadhesive gastric patch that could deliver entrapped chemotherapeutic agent for eight days after oral administration	240:374	Here, we report development of a multi-layered mucoadhesive gastric patch that could deliver entrapped chemotherapeutic agent for eight days after oral administration.					
36529359	5	64	theme	acid	600:603	arg1	conjugate					605:613	chitosan-hydrocaffeic acid conjugate	578:613	chitosan-hydrocaffeic acid conjugate	578:613	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate showed greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa.					
36529359	5	65	from	strength	644:651	arg1	mucosa					703:708	freshly excised rat gastric mucosa	675:708	freshly excised rat gastric mucosa	675:708	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate showed greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa.					
36529359	11	66	from	administration	1456:1469	arg1	mice					1574:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	12	67	theme	oral	1778:1781	arg1	patch					1738:1742	the multi-layered mucoadhesive gastric patch	1699:1742	the multi-layered mucoadhesive gastric patch	1699:1742	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	12	67	theme	oral	1778:1781	arg1	system					1797:1802	a long-acting oral drug delivery system	1764:1802	a long-acting oral drug delivery system	1764:1802	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	11	68	theme	nude	1569:1572	arg1	mice					1574:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	2	69	theme	gastric	269:275	arg1	patch					277:281	a multi-layered mucoadhesive gastric patch	240:281	a multi-layered mucoadhesive gastric patch that could deliver entrapped chemotherapeutic agent for eight days after oral administration	240:374	Here, we report development of a multi-layered mucoadhesive gastric patch that could deliver entrapped chemotherapeutic agent for eight days after oral administration.					
36529359	11	70	from	reduction	1610:1618	arg1	volume					1633:1638	the tumor volume	1623:1638	the tumor volume over 7 days	1623:1650	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	12	71	theme	delivery	1788:1795	arg1	patch					1738:1742	the multi-layered mucoadhesive gastric patch	1699:1742	the multi-layered mucoadhesive gastric patch	1699:1742	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	12	71	theme	delivery	1788:1795	arg1	system					1797:1802	a long-acting oral drug delivery system	1764:1802	a long-acting oral drug delivery system	1764:1802	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	0	72	theme	model	98:102	arg1	agent					121:125	model chemotherapeutic agent	98:125	model chemotherapeutic agent	98:125	Hydrocaffeic acid-chitosan coating of gastric patch provides long-acting mucoadhesive delivery of model chemotherapeutic agent.					
36529359	1	73	theme	dosage	181:186	arg1	form					188:191	a long-acting orally administered dosage form	147:191	a long-acting orally administered dosage form	147:191	The development of a long-acting orally administered dosage form is a challenge.					
36529359	11	74	theme	significant	1589:1599	arg1	reduction					1610:1618	significant (P<0.05) reduction	1589:1618	significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group	1589:1680	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	0	75	theme	agent	121:125	arg1	delivery					86:93	long-acting mucoadhesive delivery	61:93	long-acting mucoadhesive delivery of model chemotherapeutic agent	61:125	Hydrocaffeic acid-chitosan coating of gastric patch provides long-acting mucoadhesive delivery of model chemotherapeutic agent.					
36529359	4	76	theme	model	504:508	arg1	regorafenib					534:544	regorafenib	534:544	regorafenib	534:544	The core layer contained the model chemotherapeutic agent, regorafenib.					
36529359	4	76	theme	model	504:508	arg1	agent					527:531	the model chemotherapeutic agent	500:531	the model chemotherapeutic agent	500:531	The core layer contained the model chemotherapeutic agent, regorafenib.					
36529359	11	77	theme	FaDu	1525:1528	arg1	mice					1574:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	FaDu cell xenografted tumor bearing athymic nude mice	1525:1577	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	8	78	theme	storage	1122:1128	arg1	conditions					1130:1139	long term storage conditions	1112:1139	long term storage conditions	1112:1139	The patches were found to be stable for desired characteristics for up to 3 months in long term storage conditions.					
36529359	5	79	theme	kPa	668:670	arg1	strength					644:651	greatest mucoadhesion strength	622:651	greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa	622:708	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate showed greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa.					
36529359	12	80	theme	gastric	1730:1736	arg1	patch					1738:1742	the multi-layered mucoadhesive gastric patch	1699:1742	the multi-layered mucoadhesive gastric patch	1699:1742	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	12	80	theme	gastric	1730:1736	arg1	system					1797:1802	a long-acting oral drug delivery system	1764:1802	a long-acting oral drug delivery system	1764:1802	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	3	81	theme	multi-layered	381:393	arg1	patch					395:399	The multi-layered patch	377:399	The multi-layered patch	377:399	The multi-layered patch was designed to contain core layer, mucoadhesive layer and backing layer.					
36529359	11	82	dep	significant	1589:1599	arg1	P<0.05					1602:1607	P<0.05	1602:1607	P<0.05	1602:1607	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	6	83	theme	polycaprolactone-polydimethylsiloxane	749:785	arg1	composite					787:795	hydrophobic polycaprolactone-polydimethylsiloxane composite	737:795	hydrophobic polycaprolactone-polydimethylsiloxane composite	737:795	The backing layer made of hydrophobic polycaprolactone-polydimethylsiloxane composite showed the contact angle of 120 ± 4.7° after placement of water drop.					
36529359	0	84	theme	acid-chitosan	13:25	arg1	coating					27:33	Hydrocaffeic acid-chitosan coating	0:33	Hydrocaffeic acid-chitosan coating of gastric patch	0:50	Hydrocaffeic acid-chitosan coating of gastric patch provides long-acting mucoadhesive delivery of model chemotherapeutic agent.					
36529359	5	85	theme	mucoadhesion	631:642	arg1	strength					644:651	greatest mucoadhesion strength	622:651	greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa	622:708	The mucoadhesive layer made of chitosan-hydrocaffeic acid conjugate showed greatest mucoadhesion strength of 18.1 ± 0.78 kPa in freshly excised rat gastric mucosa.					
36529359	3	86	theme	core	425:428	arg1	layer					468:472	backing layer	460:472	backing layer	460:472	The multi-layered patch was designed to contain core layer, mucoadhesive layer and backing layer.					
36529359	3	86	theme	core	425:428	arg1	layer					430:434	core layer	425:434	core layer	425:434	The multi-layered patch was designed to contain core layer, mucoadhesive layer and backing layer.					
36529359	3	86	theme	core	425:428	arg1	layer					450:454	mucoadhesive layer	437:454	mucoadhesive layer	437:454	The multi-layered patch was designed to contain core layer, mucoadhesive layer and backing layer.					
36529359	9	87	theme	rat	1173:1175	arg1	model					1177:1181	rat model	1173:1181	rat model	1173:1181	The pharmacokinetic studies in rat model revealed constant plasma concentration of regorafenib sustained for 8 days after oral administration of gastric patch.					
36529359	3	88	theme	backing	460:466	arg1	layer					468:472	backing layer	460:472	backing layer	460:472	The multi-layered patch was designed to contain core layer, mucoadhesive layer and backing layer.					
36529359	3	88	theme	backing	460:466	arg1	layer					430:434	core layer	425:434	core layer	425:434	The multi-layered patch was designed to contain core layer, mucoadhesive layer and backing layer.					
36529359	10	89	theme	significant	1373:1383	arg1	changes					1398:1404	any significant histological changes	1369:1404	any significant histological changes	1369:1404	The gastric tissue where the patch adhered for 8 days did not show any significant histological changes compared with the normal gastric tissue.					
36529359	4	90	contain	contained	490:498	arg2	agent					527:531	the model chemotherapeutic agent	500:531	the model chemotherapeutic agent	500:531	The core layer contained the model chemotherapeutic agent, regorafenib.					
36529359	4	90	contain	contained	490:498	arg2	regorafenib					534:544	regorafenib	534:544	regorafenib	534:544	The core layer contained the model chemotherapeutic agent, regorafenib.					
36529359	4	90	contain	contained	490:498	arg1	layer					484:488	The core layer	475:488	The core layer	475:488	The core layer contained the model chemotherapeutic agent, regorafenib.					
36529359	0	91	theme	patch	46:50	arg1	coating					27:33	Hydrocaffeic acid-chitosan coating	0:33	Hydrocaffeic acid-chitosan coating of gastric patch	0:50	Hydrocaffeic acid-chitosan coating of gastric patch provides long-acting mucoadhesive delivery of model chemotherapeutic agent.					
36529359	9	92	theme	pharmacokinetic	1146:1160	arg1	studies					1162:1168	The pharmacokinetic studies	1142:1168	The pharmacokinetic studies in rat model	1142:1181	The pharmacokinetic studies in rat model revealed constant plasma concentration of regorafenib sustained for 8 days after oral administration of gastric patch.					
36529359	12	93	theme	multi-layered	1703:1715	arg1	patch					1738:1742	the multi-layered mucoadhesive gastric patch	1699:1742	the multi-layered mucoadhesive gastric patch	1699:1742	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	12	93	theme	multi-layered	1703:1715	arg1	system					1797:1802	a long-acting oral drug delivery system	1764:1802	a long-acting oral drug delivery system	1764:1802	Taken together, the multi-layered mucoadhesive gastric patch can be developed as a long-acting oral drug delivery system.					
36529359	9	94	theme	plasma	1201:1206	arg1	concentration					1208:1220	constant plasma concentration	1192:1220	constant plasma concentration of regorafenib sustained for 8 days after oral administration of gastric patch	1192:1299	The pharmacokinetic studies in rat model revealed constant plasma concentration of regorafenib sustained for 8 days after oral administration of gastric patch.					
36529359	0	95	theme	long-acting	61:71	arg1	delivery					86:93	long-acting mucoadhesive delivery	61:93	long-acting mucoadhesive delivery of model chemotherapeutic agent	61:125	Hydrocaffeic acid-chitosan coating of gastric patch provides long-acting mucoadhesive delivery of model chemotherapeutic agent.					
36529359	6	96	theme	±	829:829	arg1	angle					816:820	the contact angle	804:820	the contact angle of 120 ±	804:829	The backing layer made of hydrophobic polycaprolactone-polydimethylsiloxane composite showed the contact angle of 120 ± 4.7° after placement of water drop.					
36529359	11	97	theme	patch	1516:1520	arg1	dose					1481:1484	single dose	1474:1484	single dose of regorafenib-loaded gastric patch	1474:1520	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	11	98	theme	single	1474:1479	arg1	dose					1481:1484	single dose	1474:1484	single dose of regorafenib-loaded gastric patch	1474:1520	The oral administration of single dose of regorafenib-loaded gastric patch in FaDu cell xenografted tumor bearing athymic nude mice has shown significant (P<0.05) reduction in the tumor volume over 7 days compared to the control group.					
36529359	6	99	theme	contact	808:814	arg1	angle					816:820	the contact angle	804:820	the contact angle of 120 ±	804:829	The backing layer made of hydrophobic polycaprolactone-polydimethylsiloxane composite showed the contact angle of 120 ± 4.7° after placement of water drop.					
36529359	7	100	theme	gastric	971:977	arg1	fluid					979:983	simulated gastric fluid	961:983	simulated gastric fluid	961:983	The entrapped regorafenib predominantly released from the mucoadhesive-side of the patch into simulated gastric fluid and showed a zero-order release profile.					
36192523	3	0	theme	model	332:336	arg1	horses					320:325	elite endurance horses	304:325	elite endurance horses	304:325	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	3	0	theme	model	332:336	arg1	system					338:343	a model system	330:343	a model system for exercise responsiveness	330:371	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	6	1	theme	increased	1003:1011	arg1	capacity					1025:1032	increased ß-oxidation capacity	1003:1032	increased ß-oxidation capacity in the mitochondria	1003:1052	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	7	2	theme	cardiovascular	1317:1330	arg1	capacity					1332:1339	cardiovascular capacity	1317:1339	cardiovascular capacity	1317:1339	In line with this hypothesis, more fit athletes show upregulation of mitochondrial-related genes involved in energy metabolism, biogenesis, and Ca2+ cytosolic transport, all of which are necessary to improve aerobic work power, spare glycogen usage, and enhance cardiovascular capacity.					
36192523	0	3	theme	cardiovascular	76:89	arg1	fitness					91:97	cardiovascular fitness	76:97	cardiovascular fitness in endurance athletes	76:119	Mining the equine gut metagenome: poorly-characterized taxa associated with cardiovascular fitness in endurance athletes.					
36192523	5	4	theme	gut	679:681	arg1	microbiomes					683:693	gut microbiomes	679:693	gut microbiomes enriched in Lachnospiraceae taxa	679:726	The holo-omics approach shows that gut microbiomes enriched in Lachnospiraceae taxa are negatively associated with cardiovascular capacity.					
36192523	6	5	theme	long-chain	924:933	arg1	acylcarnitines					935:948	long-chain acylcarnitines	924:948	long-chain acylcarnitines	924:948	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	4	6	theme	dietary	604:610	arg1	resources					633:641	dietary, microbial, and host resources	604:641	dietary, microbial, and host resources	604:641	This catalog represents 4179 genera spanning 95 phyla and functional capacities primed to exploit energy from dietary, microbial, and host resources.					
36192523	0	7	theme	endurance	102:110	arg1	athletes					112:119	endurance athletes	102:119	endurance athletes	102:119	Mining the equine gut metagenome: poorly-characterized taxa associated with cardiovascular fitness in endurance athletes.					
36192523	7	8	theme	cytosolic	1204:1212	arg1	transport					1214:1222	Ca2+ cytosolic transport	1199:1222	Ca2+ cytosolic transport	1199:1222	In line with this hypothesis, more fit athletes show upregulation of mitochondrial-related genes involved in energy metabolism, biogenesis, and Ca2+ cytosolic transport, all of which are necessary to improve aerobic work power, spare glycogen usage, and enhance cardiovascular capacity.					
36192523	6	9	theme	glucose	873:879	arg1	concentrations					881:894	higher glucose concentrations	866:894	higher glucose concentrations	866:894	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	8	10	theme	gene	1448:1451	arg1	function					1453:1460	gene function	1448:1460	gene function	1448:1460	The results identify an associative link between endurance performance and gut microbiome composition and gene function, laying the basis for nutritional interventions that could benefit horse athletes.					
36192523	6	11	from	concentrations	881:894	arg1	plasma					984:989	plasma	984:989	plasma	984:989	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	7	12	theme	glycogen	1289:1296	arg1	usage					1298:1302	glycogen usage	1289:1302	glycogen usage	1289:1302	In line with this hypothesis, more fit athletes show upregulation of mitochondrial-related genes involved in energy metabolism, biogenesis, and Ca2+ cytosolic transport, all of which are necessary to improve aerobic work power, spare glycogen usage, and enhance cardiovascular capacity.					
36192523	6	13	theme	higher	866:871	arg1	concentrations					881:894	higher glucose concentrations	866:894	higher glucose concentrations	866:894	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	4	14	theme	microbial	613:621	arg1	resources					633:641	dietary, microbial, and host resources	604:641	dietary, microbial, and host resources	604:641	This catalog represents 4179 genera spanning 95 phyla and functional capacities primed to exploit energy from dietary, microbial, and host resources.					
36192523	1	15	theme	exercise	199:206	arg1	performance					208:218	endurance exercise performance	189:218	endurance exercise performance	189:218	Emerging evidence indicates that the gut microbiome contributes to endurance exercise performance.					
36192523	6	16	from	accumulation	908:919	arg1	plasma					984:989	plasma	984:989	plasma	984:989	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	6	17	theme	acids	975:979	arg1	accumulation					908:919	reduced accumulation	900:919	reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma	900:989	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	6	17	theme	acids	975:979	arg1	concentrations					881:894	higher glucose concentrations	866:894	higher glucose concentrations	866:894	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	5	18	theme	holo-omics	648:657	arg1	approach					659:666	The holo-omics approach	644:666	The holo-omics approach	644:666	The holo-omics approach shows that gut microbiomes enriched in Lachnospiraceae taxa are negatively associated with cardiovascular capacity.					
36192523	7	19	theme	work	1271:1274	arg1	power					1276:1280	aerobic work power	1263:1280	aerobic work power	1263:1280	In line with this hypothesis, more fit athletes show upregulation of mitochondrial-related genes involved in energy metabolism, biogenesis, and Ca2+ cytosolic transport, all of which are necessary to improve aerobic work power, spare glycogen usage, and enhance cardiovascular capacity.					
36192523	1	20	theme	endurance	189:197	arg1	performance					208:218	endurance exercise performance	189:218	endurance exercise performance	189:218	Emerging evidence indicates that the gut microbiome contributes to endurance exercise performance.					
36192523	3	21	theme	horse	397:401	arg1	catalog					412:418	an integrated horse gut gene catalog	383:418	an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes	383:491	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	6	22	theme	fatty	969:973	arg1	acids					975:979	non-esterified fatty acids	954:979	non-esterified fatty acids	954:979	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	6	23	from	capacity	1025:1032	arg1	mitochondria					1041:1052	the mitochondria	1037:1052	the mitochondria	1037:1052	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	3	24	theme	million	435:441	arg1	genes					450:454	~25 million unique genes	431:454	~25 million unique genes	431:454	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	3	25	theme	gut	403:405	arg1	catalog					412:418	an integrated horse gut gene catalog	383:418	an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes	383:491	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	7	26	theme	energy	1164:1169	arg1	metabolism					1171:1180	energy metabolism	1164:1180	energy metabolism	1164:1180	In line with this hypothesis, more fit athletes show upregulation of mitochondrial-related genes involved in energy metabolism, biogenesis, and Ca2+ cytosolic transport, all of which are necessary to improve aerobic work power, spare glycogen usage, and enhance cardiovascular capacity.					
36192523	3	27	theme	unique	443:448	arg1	genes					450:454	~25 million unique genes	431:454	~25 million unique genes	431:454	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	0	28	theme	gut	18:20	arg1	metagenome					22:31	the equine gut metagenome	7:31	the equine gut metagenome	7:31	Mining the equine gut metagenome: poorly-characterized taxa associated with cardiovascular fitness in endurance athletes.					
36192523	6	29	theme	diverse	826:832	arg1	microbiomes					834:844	more complex and functionally diverse microbiomes	796:844	more complex and functionally diverse microbiomes	796:844	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	3	30	theme	integrated	386:395	arg1	catalog					412:418	an integrated horse gut gene catalog	383:418	an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes	383:491	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	3	31	theme	elite	304:308	arg1	horses					320:325	elite endurance horses	304:325	elite endurance horses	304:325	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	3	31	theme	elite	304:308	arg1	system					338:343	a model system	330:343	a model system for exercise responsiveness	330:371	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	0	32	from	fitness	91:97	arg1	athletes					112:119	endurance athletes	102:119	endurance athletes	102:119	Mining the equine gut metagenome: poorly-characterized taxa associated with cardiovascular fitness in endurance athletes.					
36192523	8	33	theme	microbiome	1421:1430	arg1	composition					1432:1442	gut microbiome composition	1417:1442	gut microbiome composition	1417:1442	The results identify an associative link between endurance performance and gut microbiome composition and gene function, laying the basis for nutritional interventions that could benefit horse athletes.					
36192523	8	34	theme	gut	1417:1419	arg1	composition					1432:1442	gut microbiome composition	1417:1442	gut microbiome composition	1417:1442	The results identify an associative link between endurance performance and gut microbiome composition and gene function, laying the basis for nutritional interventions that could benefit horse athletes.					
36192523	2	35	theme	functional	246:255	arg1	potential					271:279	its functional and metabolic potential	242:279	its functional and metabolic potential	242:279	Still, the extent of its functional and metabolic potential remains unknown.					
36192523	8	36	theme	associative	1366:1376	arg1	link					1378:1381	an associative link	1363:1381	an associative link between endurance performance and gut microbiome composition and gene function	1363:1460	The results identify an associative link between endurance performance and gut microbiome composition and gene function, laying the basis for nutritional interventions that could benefit horse athletes.					
36192523	0	37	theme	equine	11:16	arg1	metagenome					22:31	the equine gut metagenome	7:31	the equine gut metagenome	7:31	Mining the equine gut metagenome: poorly-characterized taxa associated with cardiovascular fitness in endurance athletes.					
36192523	4	38	theme	functional	552:561	arg1	capacities					563:572	functional capacities	552:572	functional capacities	552:572	This catalog represents 4179 genera spanning 95 phyla and functional capacities primed to exploit energy from dietary, microbial, and host resources.					
36192523	5	39	theme	cardiovascular	759:772	arg1	capacity					774:781	cardiovascular capacity	759:781	cardiovascular capacity	759:781	The holo-omics approach shows that gut microbiomes enriched in Lachnospiraceae taxa are negatively associated with cardiovascular capacity.					
36192523	6	40	theme	acylcarnitines	935:948	arg1	accumulation					908:919	reduced accumulation	900:919	reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma	900:989	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	6	40	theme	acylcarnitines	935:948	arg1	concentrations					881:894	higher glucose concentrations	866:894	higher glucose concentrations	866:894	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	8	41	theme	nutritional	1484:1494	arg1	interventions					1496:1508	nutritional interventions	1484:1508	nutritional interventions that could benefit horse athletes	1484:1542	The results identify an associative link between endurance performance and gut microbiome composition and gene function, laying the basis for nutritional interventions that could benefit horse athletes.					
36192523	3	42	theme	metagenome-assembled	464:483	arg1	genomes					485:491	372 metagenome-assembled genomes	460:491	372 metagenome-assembled genomes	460:491	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	3	43	theme	gene	407:410	arg1	catalog					412:418	an integrated horse gut gene catalog	383:418	an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes	383:491	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	7	44	theme	genes	1146:1150	arg1	upregulation					1108:1119	upregulation	1108:1119	upregulation of mitochondrial-related genes involved in energy metabolism, biogenesis, and Ca2+ cytosolic transport, all of which are necessary to improve aerobic work power, spare glycogen usage, and enhance cardiovascular capacity	1108:1339	In line with this hypothesis, more fit athletes show upregulation of mitochondrial-related genes involved in energy metabolism, biogenesis, and Ca2+ cytosolic transport, all of which are necessary to improve aerobic work power, spare glycogen usage, and enhance cardiovascular capacity.					
36192523	4	45	from	resources	633:641	arg1	energy					592:597	energy	592:597	energy from dietary, microbial, and host resources	592:641	This catalog represents 4179 genera spanning 95 phyla and functional capacities primed to exploit energy from dietary, microbial, and host resources.					
36192523	6	46	theme	non-esterified	954:967	arg1	acids					975:979	non-esterified fatty acids	954:979	non-esterified fatty acids	954:979	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	7	47	theme	fit	1090:1092	arg1	athletes					1094:1101	more fit athletes	1085:1101	more fit athletes	1085:1101	In line with this hypothesis, more fit athletes show upregulation of mitochondrial-related genes involved in energy metabolism, biogenesis, and Ca2+ cytosolic transport, all of which are necessary to improve aerobic work power, spare glycogen usage, and enhance cardiovascular capacity.					
36192523	1	48	theme	Emerging	122:129	arg1	evidence					131:138	Emerging evidence	122:138	Emerging evidence	122:138	Emerging evidence indicates that the gut microbiome contributes to endurance exercise performance.					
36192523	0	49	theme	poorly-characterized	34:53	arg1	taxa					55:58	poorly-characterized taxa	34:58	poorly-characterized taxa associated with cardiovascular fitness in endurance athletes	34:119	Mining the equine gut metagenome: poorly-characterized taxa associated with cardiovascular fitness in endurance athletes.					
36192523	7	50	with	line	1058:1061	arg1	hypothesis					1073:1082	this hypothesis	1068:1082	this hypothesis	1068:1082	In line with this hypothesis, more fit athletes show upregulation of mitochondrial-related genes involved in energy metabolism, biogenesis, and Ca2+ cytosolic transport, all of which are necessary to improve aerobic work power, spare glycogen usage, and enhance cardiovascular capacity.					
36192523	5	51	theme	Lachnospiraceae	707:721	arg1	taxa					723:726	Lachnospiraceae taxa	707:726	Lachnospiraceae taxa	707:726	The holo-omics approach shows that gut microbiomes enriched in Lachnospiraceae taxa are negatively associated with cardiovascular capacity.					
36192523	7	52	theme	Ca2+	1199:1202	arg1	transport					1214:1222	Ca2+ cytosolic transport	1199:1222	Ca2+ cytosolic transport	1199:1222	In line with this hypothesis, more fit athletes show upregulation of mitochondrial-related genes involved in energy metabolism, biogenesis, and Ca2+ cytosolic transport, all of which are necessary to improve aerobic work power, spare glycogen usage, and enhance cardiovascular capacity.					
36192523	6	53	theme	complex	801:807	arg1	microbiomes					834:844	more complex and functionally diverse microbiomes	796:844	more complex and functionally diverse microbiomes	796:844	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	3	54	theme	endurance	310:318	arg1	horses					320:325	elite endurance horses	304:325	elite endurance horses	304:325	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	3	54	theme	endurance	310:318	arg1	system					338:343	a model system	330:343	a model system for exercise responsiveness	330:371	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	8	55	theme	horse	1529:1533	arg1	athletes					1535:1542	horse athletes	1529:1542	horse athletes	1529:1542	The results identify an associative link between endurance performance and gut microbiome composition and gene function, laying the basis for nutritional interventions that could benefit horse athletes.					
36192523	4	56	theme	host	628:631	arg1	resources					633:641	dietary, microbial, and host resources	604:641	dietary, microbial, and host resources	604:641	This catalog represents 4179 genera spanning 95 phyla and functional capacities primed to exploit energy from dietary, microbial, and host resources.					
36192523	7	57	theme	mitochondrial-related	1124:1144	arg1	genes					1146:1150	mitochondrial-related genes	1124:1150	mitochondrial-related genes	1124:1150	In line with this hypothesis, more fit athletes show upregulation of mitochondrial-related genes involved in energy metabolism, biogenesis, and Ca2+ cytosolic transport, all of which are necessary to improve aerobic work power, spare glycogen usage, and enhance cardiovascular capacity.					
36192523	6	58	theme	reduced	900:906	arg1	accumulation					908:919	reduced accumulation	900:919	reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma	900:989	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	2	59	theme	potential	271:279	arg1	extent					232:237	the extent	228:237	the extent of its functional and metabolic potential	228:279	Still, the extent of its functional and metabolic potential remains unknown.					
36192523	8	60	theme	endurance	1391:1399	arg1	performance					1401:1411	endurance performance	1391:1411	endurance performance	1391:1411	The results identify an associative link between endurance performance and gut microbiome composition and gene function, laying the basis for nutritional interventions that could benefit horse athletes.					
36192523	2	61	theme	metabolic	261:269	arg1	potential					271:279	its functional and metabolic potential	242:279	its functional and metabolic potential	242:279	Still, the extent of its functional and metabolic potential remains unknown.					
36192523	7	62	theme	aerobic	1263:1269	arg1	power					1276:1280	aerobic work power	1263:1280	aerobic work power	1263:1280	In line with this hypothesis, more fit athletes show upregulation of mitochondrial-related genes involved in energy metabolism, biogenesis, and Ca2+ cytosolic transport, all of which are necessary to improve aerobic work power, spare glycogen usage, and enhance cardiovascular capacity.					
36192523	3	63	theme	exercise	349:356	arg1	responsiveness					358:371	exercise responsiveness	349:371	exercise responsiveness	349:371	Using elite endurance horses as a model system for exercise responsiveness, we built an integrated horse gut gene catalog comprising ~25 million unique genes and 372 metagenome-assembled genomes.					
36192523	6	64	theme	ß-oxidation	1013:1023	arg1	capacity					1025:1032	increased ß-oxidation capacity	1003:1032	increased ß-oxidation capacity in the mitochondria	1003:1052	Conversely, more complex and functionally diverse microbiomes are associated with higher glucose concentrations and reduced accumulation of long-chain acylcarnitines and non-esterified fatty acids in plasma, suggesting increased ß-oxidation capacity in the mitochondria.					
36192523	0	65	dep	Mining	0:5	arg1	taxa					55:58	poorly-characterized taxa	34:58	poorly-characterized taxa associated with cardiovascular fitness in endurance athletes	34:119	Mining the equine gut metagenome: poorly-characterized taxa associated with cardiovascular fitness in endurance athletes.					
36192523	1	66	theme	gut	159:161	arg1	microbiome					163:172	the gut microbiome	155:172	the gut microbiome	155:172	Emerging evidence indicates that the gut microbiome contributes to endurance exercise performance.					
35341942	6	0	theme	rheological	879:889	arg1	measurements					891:902	rheological measurements	879:902	rheological measurements	879:902	In situ gelation and the effect of the CD immobilization on this behaviour were characterized by rheological measurements.					
35341942	7	1	theme	kinetic	952:958	arg1	measurements					971:982	kinetic solubility measurements	952:982	kinetic solubility measurements	952:982	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	4	2	theme	corresponding	625:637	arg1	derivatives					644:654	the corresponding PASP derivatives	621:654	the corresponding PASP derivatives	621:654	Finally, the PSI derivatives were hydrolysed to the corresponding PASP derivatives to get water-soluble polymers.					
35341942	5	3	theme	complexation	726:737	arg1	ability					739:745	the complexation ability	722:745	the complexation ability of CD-containing PASP derivatives	722:779	Phase-solubility studies confirmed the complexation ability of CD-containing PASP derivatives.					
35341942	7	4	theme	flux	1112:1115	arg1	values					1117:1122	flux values	1112:1122	flux values	1112:1122	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	2	5	theme	dual	312:315	arg1	functionality					317:329	dual functionality	312:329	dual functionality	312:329	Poly(aspartic acid) (PASP) derivatives were synthesized with dual functionality to improve the solubility of DXM, and to achieve in situ gelation.					
35341942	1	6	theme	eye	241:243	arg1	drop					245:248	a potential in situ gellable dexamethasone (DXM) eye drop	192:248	a potential in situ gellable dexamethasone (DXM) eye drop	192:248	The present study aimed at developing a potential in situ gellable dexamethasone (DXM) eye drop.					
35341942	0	7	theme	aspartic	93:100	arg1	poly					88:91	poly	88:91	poly(aspartic acid)	88:106	Synthesis, complex formation and corneal permeation of cyclodextrin-modified, thiolated poly(aspartic acid) as self-gelling formulation of dexamethasone.					
35341942	0	7	theme	aspartic	93:100	arg1	acid					102:105	aspartic acid	93:105	aspartic acid	93:105	Synthesis, complex formation and corneal permeation of cyclodextrin-modified, thiolated poly(aspartic acid) as self-gelling formulation of dexamethasone.					
35341942	6	8	theme	In	782:783	arg1	gelation					790:797	In situ gelation	782:797	In situ gelation	782:797	In situ gelation and the effect of the CD immobilization on this behaviour were characterized by rheological measurements.					
35341942	3	9	theme	thiol	487:491	arg1	functionalities					493:507	thiol functionalities	487:507	thiol functionalities	487:507	First, amine-modified β-cyclodextrin (CD) was attached to polysuccinimide (PSI), second, thiol functionalities were added by the reaction of cysteamine and succinimide rings.					
35341942	2	10	theme	aspartic	256:263	arg1	Poly					251:254	Poly	251:254	Poly(aspartic acid) (PASP) derivatives	251:288	Poly(aspartic acid) (PASP) derivatives were synthesized with dual functionality to improve the solubility of DXM, and to achieve in situ gelation.					
35341942	2	10	theme	aspartic	256:263	arg1	acid					265:268	aspartic acid	256:268	aspartic acid	256:268	Poly(aspartic acid) (PASP) derivatives were synthesized with dual functionality to improve the solubility of DXM, and to achieve in situ gelation.					
35341942	8	11	theme	derivatives	1148:1158	arg1	effect					1129:1134	The effect	1125:1134	The effect of the PASP derivatives on permeation	1125:1172	The effect of the PASP derivatives on permeation strongly depended on chemical composition and polymer concentration.					
35341942	7	12	theme	CD	932:933	arg1	effect					922:927	The solubilizing effect	905:927	The solubilizing effect of CD	905:933	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	2	13	theme	DXM	360:362	arg1	solubility					346:355	the solubility	342:355	the solubility of DXM	342:362	Poly(aspartic acid) (PASP) derivatives were synthesized with dual functionality to improve the solubility of DXM, and to achieve in situ gelation.					
35341942	5	14	theme	Phase-solubility	687:702	arg1	studies					704:710	Phase-solubility studies	687:710	Phase-solubility studies	687:710	Phase-solubility studies confirmed the complexation ability of CD-containing PASP derivatives.					
35341942	2	15	dep	in	380:381	arg1	situ					383:386	situ	383:386	situ	383:386	Poly(aspartic acid) (PASP) derivatives were synthesized with dual functionality to improve the solubility of DXM, and to achieve in situ gelation.					
35341942	7	16	theme	solubilizing	909:920	arg1	effect					922:927	The solubilizing effect	905:927	The solubilizing effect of CD	905:933	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	2	17	theme	Poly	251:254	arg1	derivatives					278:288	Poly(aspartic acid) (PASP) derivatives	251:288	Poly(aspartic acid) (PASP) derivatives	251:288	Poly(aspartic acid) (PASP) derivatives were synthesized with dual functionality to improve the solubility of DXM, and to achieve in situ gelation.					
35341942	7	18	dep	in	993:994	arg1	vitro					996:1000	vitro	996:1000	vitro	996:1000	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	8	19	theme	polymer	1220:1226	arg1	concentration					1228:1240	polymer concentration	1220:1240	polymer concentration	1220:1240	The effect of the PASP derivatives on permeation strongly depended on chemical composition and polymer concentration.					
35341942	5	20	theme	derivatives	769:779	arg1	ability					739:745	the complexation ability	722:745	the complexation ability of CD-containing PASP derivatives	722:779	Phase-solubility studies confirmed the complexation ability of CD-containing PASP derivatives.					
35341942	4	21	theme	water-soluble	663:675	arg1	polymers					677:684	water-soluble polymers	663:684	water-soluble polymers	663:684	Finally, the PSI derivatives were hydrolysed to the corresponding PASP derivatives to get water-soluble polymers.					
35341942	6	22	from	effect	807:812	arg1	behaviour					847:855	this behaviour	842:855	this behaviour	842:855	In situ gelation and the effect of the CD immobilization on this behaviour were characterized by rheological measurements.					
35341942	0	23	theme	self-gelling	111:122	arg1	formulation					124:134	self-gelling formulation	111:134	self-gelling formulation of dexamethasone	111:151	Synthesis, complex formation and corneal permeation of cyclodextrin-modified, thiolated poly(aspartic acid) as self-gelling formulation of dexamethasone.					
35341942	8	24	theme	chemical	1195:1202	arg1	composition					1204:1214	chemical composition	1195:1214	chemical composition	1195:1214	The effect of the PASP derivatives on permeation strongly depended on chemical composition and polymer concentration.					
35341942	3	25	theme	amine-modified	405:418	arg1	CD					436:437	CD	436:437	CD	436:437	First, amine-modified β-cyclodextrin (CD) was attached to polysuccinimide (PSI), second, thiol functionalities were added by the reaction of cysteamine and succinimide rings.					
35341942	3	25	theme	amine-modified	405:418	arg1	β-cyclodextrin					420:433	amine-modified β-cyclodextrin	405:433	amine-modified β-cyclodextrin (CD)	405:438	First, amine-modified β-cyclodextrin (CD) was attached to polysuccinimide (PSI), second, thiol functionalities were added by the reaction of cysteamine and succinimide rings.					
35341942	2	26	theme	in	380:381	arg1	gelation					388:395	in situ gelation	380:395	in situ gelation	380:395	Poly(aspartic acid) (PASP) derivatives were synthesized with dual functionality to improve the solubility of DXM, and to achieve in situ gelation.					
35341942	6	27	theme	immobilization	824:837	arg1	gelation					790:797	In situ gelation	782:797	In situ gelation	782:797	In situ gelation and the effect of the CD immobilization on this behaviour were characterized by rheological measurements.					
35341942	6	27	theme	immobilization	824:837	arg1	effect					807:812	the effect	803:812	the effect of the CD immobilization on this behaviour	803:855	In situ gelation and the effect of the CD immobilization on this behaviour were characterized by rheological measurements.					
35341942	1	28	theme	present	158:164	arg1	study					166:170	The present study	154:170	The present study	154:170	The present study aimed at developing a potential in situ gellable dexamethasone (DXM) eye drop.					
35341942	1	29	theme	potential	194:202	arg1	drop					245:248	a potential in situ gellable dexamethasone (DXM) eye drop	192:248	a potential in situ gellable dexamethasone (DXM) eye drop	192:248	The present study aimed at developing a potential in situ gellable dexamethasone (DXM) eye drop.					
35341942	0	30	theme	corneal	33:39	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, complex formation and corneal permeation of cyclodextrin-modified, thiolated poly(aspartic acid) as self-gelling formulation of dexamethasone.					
35341942	0	30	theme	corneal	33:39	arg1	permeation					41:50	corneal permeation	33:50	corneal permeation	33:50	Synthesis, complex formation and corneal permeation of cyclodextrin-modified, thiolated poly(aspartic acid) as self-gelling formulation of dexamethasone.					
35341942	6	31	theme	CD	821:822	arg1	immobilization					824:837	the CD immobilization	817:837	the CD immobilization	817:837	In situ gelation and the effect of the CD immobilization on this behaviour were characterized by rheological measurements.					
35341942	6	32	dep	In	782:783	arg1	situ					785:788	situ	785:788	situ	785:788	In situ gelation and the effect of the CD immobilization on this behaviour were characterized by rheological measurements.					
35341942	1	33	theme	in	204:205	arg1	DXM					236:238	DXM	236:238	DXM	236:238	The present study aimed at developing a potential in situ gellable dexamethasone (DXM) eye drop.					
35341942	1	33	theme	in	204:205	arg1	dexamethasone					221:233	in situ gellable dexamethasone	204:233	a potential in situ gellable dexamethasone (DXM) eye drop	192:248	The present study aimed at developing a potential in situ gellable dexamethasone (DXM) eye drop.					
35341942	4	34	theme	PSI	586:588	arg1	derivatives					590:600	the PSI derivatives	582:600	the PSI derivatives	582:600	Finally, the PSI derivatives were hydrolysed to the corresponding PASP derivatives to get water-soluble polymers.					
35341942	6	35	from	gelation	790:797	arg1	behaviour					847:855	this behaviour	842:855	this behaviour	842:855	In situ gelation and the effect of the CD immobilization on this behaviour were characterized by rheological measurements.					
35341942	3	36	theme	rings	566:570	arg1	reaction					527:534	the reaction	523:534	the reaction of cysteamine and succinimide rings	523:570	First, amine-modified β-cyclodextrin (CD) was attached to polysuccinimide (PSI), second, thiol functionalities were added by the reaction of cysteamine and succinimide rings.					
35341942	7	37	theme	in	993:994	arg1	assay					1023:1027	in vitro corneal permeability assay	993:1027	in vitro corneal permeability assay (corneal-PAMPA) measurements	993:1056	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	7	37	theme	in	993:994	arg1	corneal-PAMPA					1030:1042	corneal-PAMPA	1030:1042	corneal-PAMPA	1030:1042	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	8	38	theme	PASP	1143:1146	arg1	derivatives					1148:1158	the PASP derivatives	1139:1158	the PASP derivatives	1139:1158	The effect of the PASP derivatives on permeation strongly depended on chemical composition and polymer concentration.					
35341942	5	39	theme	CD-containing	750:762	arg1	derivatives					769:779	CD-containing PASP derivatives	750:779	CD-containing PASP derivatives	750:779	Phase-solubility studies confirmed the complexation ability of CD-containing PASP derivatives.					
35341942	7	40	theme	assay	1023:1027	arg1	measurements					1045:1056	in vitro corneal permeability assay (corneal-PAMPA) measurements	993:1056	in vitro corneal permeability assay (corneal-PAMPA) measurements	993:1056	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	8	41	from	effect	1129:1134	arg1	permeation					1163:1172	permeation	1163:1172	permeation	1163:1172	The effect of the PASP derivatives on permeation strongly depended on chemical composition and polymer concentration.					
35341942	5	42	theme	PASP	764:767	arg1	derivatives					769:779	CD-containing PASP derivatives	750:779	CD-containing PASP derivatives	750:779	Phase-solubility studies confirmed the complexation ability of CD-containing PASP derivatives.					
35341942	0	43	theme	dexamethasone	139:151	arg1	formulation					124:134	self-gelling formulation	111:134	self-gelling formulation of dexamethasone	111:151	Synthesis, complex formation and corneal permeation of cyclodextrin-modified, thiolated poly(aspartic acid) as self-gelling formulation of dexamethasone.					
35341942	4	44	theme	PASP	639:642	arg1	derivatives					644:654	the corresponding PASP derivatives	621:654	the corresponding PASP derivatives	621:654	Finally, the PSI derivatives were hydrolysed to the corresponding PASP derivatives to get water-soluble polymers.					
35341942	3	45	theme	cysteamine	539:548	arg1	reaction					527:534	the reaction	523:534	the reaction of cysteamine and succinimide rings	523:570	First, amine-modified β-cyclodextrin (CD) was attached to polysuccinimide (PSI), second, thiol functionalities were added by the reaction of cysteamine and succinimide rings.					
35341942	7	46	theme	corneal	1002:1008	arg1	assay					1023:1027	in vitro corneal permeability assay	993:1027	in vitro corneal permeability assay (corneal-PAMPA) measurements	993:1056	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	7	46	theme	corneal	1002:1008	arg1	corneal-PAMPA					1030:1042	corneal-PAMPA	1030:1042	corneal-PAMPA	1030:1042	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	1	47	dep	in	204:205	arg1	situ					207:210	situ	207:210	situ	207:210	The present study aimed at developing a potential in situ gellable dexamethasone (DXM) eye drop.					
35341942	3	48	attach	attached	444:451	arg2	β-cyclodextrin					420:433	amine-modified β-cyclodextrin	405:433	amine-modified β-cyclodextrin (CD)	405:438	First, amine-modified β-cyclodextrin (CD) was attached to polysuccinimide (PSI), second, thiol functionalities were added by the reaction of cysteamine and succinimide rings.					
35341942	3	48	attach	attached	444:451	arg1	PSI					473:475	PSI	473:475	PSI	473:475	First, amine-modified β-cyclodextrin (CD) was attached to polysuccinimide (PSI), second, thiol functionalities were added by the reaction of cysteamine and succinimide rings.					
35341942	3	48	attach	attached	444:451	arg1	polysuccinimide					456:470	polysuccinimide	456:470	polysuccinimide (PSI)	456:476	First, amine-modified β-cyclodextrin (CD) was attached to polysuccinimide (PSI), second, thiol functionalities were added by the reaction of cysteamine and succinimide rings.					
35341942	3	48	attach	attached	444:451	arg2	CD					436:437	CD	436:437	CD	436:437	First, amine-modified β-cyclodextrin (CD) was attached to polysuccinimide (PSI), second, thiol functionalities were added by the reaction of cysteamine and succinimide rings.					
35341942	1	49	theme	gellable	212:219	arg1	DXM					236:238	DXM	236:238	DXM	236:238	The present study aimed at developing a potential in situ gellable dexamethasone (DXM) eye drop.					
35341942	1	49	theme	gellable	212:219	arg1	dexamethasone					221:233	in situ gellable dexamethasone	204:233	a potential in situ gellable dexamethasone (DXM) eye drop	192:248	The present study aimed at developing a potential in situ gellable dexamethasone (DXM) eye drop.					
35341942	7	50	theme	permeability	1010:1021	arg1	assay					1023:1027	in vitro corneal permeability assay	993:1027	in vitro corneal permeability assay (corneal-PAMPA) measurements	993:1056	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	7	50	theme	permeability	1010:1021	arg1	corneal-PAMPA					1030:1042	corneal-PAMPA	1030:1042	corneal-PAMPA	1030:1042	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	3	51	theme	succinimide	554:564	arg1	rings					566:570	succinimide rings	554:570	succinimide rings	554:570	First, amine-modified β-cyclodextrin (CD) was attached to polysuccinimide (PSI), second, thiol functionalities were added by the reaction of cysteamine and succinimide rings.					
35341942	7	52	theme	solubility	960:969	arg1	measurements					971:982	kinetic solubility measurements	952:982	kinetic solubility measurements	952:982	The solubilizing effect of CD was confirmed by kinetic solubility measurements, whereas in vitro corneal permeability assay (corneal-PAMPA) measurements were performed to determine in vitro permeability and flux values.					
35341942	1	53	theme	dexamethasone	221:233	arg1	drop					245:248	a potential in situ gellable dexamethasone (DXM) eye drop	192:248	a potential in situ gellable dexamethasone (DXM) eye drop	192:248	The present study aimed at developing a potential in situ gellable dexamethasone (DXM) eye drop.					
35341942	0	54	theme	cyclodextrin-modified	55:75	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, complex formation and corneal permeation of cyclodextrin-modified, thiolated poly(aspartic acid) as self-gelling formulation of dexamethasone.					
35341942	0	54	theme	cyclodextrin-modified	55:75	arg1	formation					19:27	complex formation	11:27	complex formation	11:27	Synthesis, complex formation and corneal permeation of cyclodextrin-modified, thiolated poly(aspartic acid) as self-gelling formulation of dexamethasone.					
35341942	0	54	theme	cyclodextrin-modified	55:75	arg1	permeation					41:50	corneal permeation	33:50	corneal permeation	33:50	Synthesis, complex formation and corneal permeation of cyclodextrin-modified, thiolated poly(aspartic acid) as self-gelling formulation of dexamethasone.					
35341942	0	55	theme	complex	11:17	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, complex formation and corneal permeation of cyclodextrin-modified, thiolated poly(aspartic acid) as self-gelling formulation of dexamethasone.					
35341942	0	55	theme	complex	11:17	arg1	formation					19:27	complex formation	11:27	complex formation	11:27	Synthesis, complex formation and corneal permeation of cyclodextrin-modified, thiolated poly(aspartic acid) as self-gelling formulation of dexamethasone.					
36417060	10	0	dep	%	1751:1751	arg1	3					1750:1750	3	1750:1750	3	1750:1750	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	3	1	theme	optimal	474:480	arg1	conditions					482:491	the optimal conditions	470:491	the optimal conditions for formaldehyde removal using Alg@Fe3O4@Bent nanocomposite	470:551	The central composite design method was employed to find the optimal conditions for formaldehyde removal using Alg@Fe3O4@Bent nanocomposite.					
36417060	6	2	theme	Langmuir	1051:1058	arg1	isotherm					1060:1067	the Langmuir isotherm	1047:1067	the Langmuir isotherm	1047:1067	The isotherm and kinetic investigations demonstrated that the formaldehyde uptake process by the foresaid adsorbent follows the Langmuir isotherm and quasi-first-order kinetic models, respectively.					
36417060	2	3	theme	bentonite	274:282	arg1	features					262:269	Structural features	251:269	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite	251:329	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	1	4	theme	Alginate	155:162	arg1	nanocomposite					181:193	Alginate@ Fe3O4/Bentonite nanocomposite	155:193	Alginate@ Fe3O4/Bentonite nanocomposite	155:193	In this study, Alginate@ Fe3O4/Bentonite nanocomposite was utilized to eliminate formaldehyde from wastewater.					
36417060	1	5	theme	Fe3O4/Bentonite	165:179	arg1	nanocomposite					181:193	Alginate@ Fe3O4/Bentonite nanocomposite	155:193	Alginate@ Fe3O4/Bentonite nanocomposite	155:193	In this study, Alginate@ Fe3O4/Bentonite nanocomposite was utilized to eliminate formaldehyde from wastewater.					
36417060	10	6	theme	nitrate	1688:1694	arg1	ion					1696:1698	nitrate ion	1688:1698	nitrate ion	1688:1698	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	4	7	theme	maximum	558:564	arg1	efficiency					586:595	The maximum formaldehyde uptake efficiency	554:595	The maximum formaldehyde uptake efficiency (94.56%)	554:604	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	4	7	theme	maximum	558:564	arg1	%					603:603	94.56%	598:603	94.56%	598:603	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	2	8	theme	Fe3O4	295:299	arg1	features					262:269	Structural features	251:269	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite	251:329	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	5	9	theme	@	745:745	arg1	composite					762:770	Alginate@Fe3O4@Bentonite composite	737:770	Alginate@Fe3O4@Bentonite composite	737:770	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	4	10	theme	uptake	579:584	arg1	efficiency					586:595	The maximum formaldehyde uptake efficiency	554:595	The maximum formaldehyde uptake efficiency (94.56%)	554:604	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	4	10	theme	uptake	579:584	arg1	%					603:603	94.56%	598:603	94.56%	598:603	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	0	11	theme	composite	122:130	arg1	design					132:137	central composite design	114:137	central composite design	114:137	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.					
36417060	3	12	theme	Fe3O4	528:532	arg1	nanocomposite					539:551	Alg@Fe3O4@Bent nanocomposite	524:551	Alg@Fe3O4@Bent nanocomposite	524:551	The central composite design method was employed to find the optimal conditions for formaldehyde removal using Alg@Fe3O4@Bent nanocomposite.					
36417060	5	13	theme	@	751:751	arg1	composite					762:770	Alginate@Fe3O4@Bentonite composite	737:770	Alginate@Fe3O4@Bentonite composite	737:770	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	10	14	with	interaction	1709:1719	arg1	formaldehyde					1726:1737	formaldehyde	1726:1737	formaldehyde (less than 3% reduction in removal efficiency)	1726:1784	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	10	15	theme	interfering	1515:1525	arg1	ions					1527:1530	interfering ions	1515:1530	interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate	1515:1588	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	3	16	theme	Alg	524:526	arg1	nanocomposite					539:551	Alg@Fe3O4@Bent nanocomposite	524:551	Alg@Fe3O4@Bent nanocomposite	524:551	The central composite design method was employed to find the optimal conditions for formaldehyde removal using Alg@Fe3O4@Bent nanocomposite.					
36417060	5	17	theme	petrochemical	817:829	arg1	wastewater					831:840	Razi petrochemical wastewater	812:840	Razi petrochemical wastewater	812:840	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	4	18	theme	10.69 ppm	652:660	arg1	concentration					635:647	formaldehyde concentration	622:647	formaldehyde concentration of 10.69 ppm	622:660	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	4	18	theme	10.69 ppm	652:660	arg1	pH					703:704	pH	703:704	pH of 9.96	703:712	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	4	18	theme	10.69 ppm	652:660	arg1	dose					681:684	the nanocomposite dose	663:684	the nanocomposite dose of 1.28 g/L	663:696	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	5	19	theme	BOD5	917:920	arg1	%					894:894	70%	892:894	70% of COD	892:901	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	19	theme	BOD5	917:920	arg1	%					912:912	68.9%	908:912	68.9% of BOD5	908:920	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	19	theme	BOD5	917:920	arg1	BOD5					917:920	BOD5	917:920	BOD5	917:920	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	19	theme	BOD5	917:920	arg1	%					873:873	91.24%	868:873	91.24% of formaldehyde	868:889	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	19	theme	BOD5	917:920	arg1	COD					899:901	COD	899:901	COD	899:901	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	19	theme	BOD5	917:920	arg1	formaldehyde					878:889	formaldehyde	878:889	formaldehyde	878:889	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	6	20	theme	kinetic	1091:1097	arg1	models					1099:1104	quasi-first-order kinetic models	1073:1104	quasi-first-order kinetic models	1073:1104	The isotherm and kinetic investigations demonstrated that the formaldehyde uptake process by the foresaid adsorbent follows the Langmuir isotherm and quasi-first-order kinetic models, respectively.					
36417060	8	21	theme	uptake	1210:1215	arg1	process					1217:1223	the formaldehyde uptake process	1193:1223	the formaldehyde uptake process by the aforementioned nanocomposite	1193:1259	Moreover, the formaldehyde uptake process by the aforementioned nanocomposite was exothermic and spontaneous.					
36417060	8	21	theme	uptake	1210:1215	arg1	exothermic					1265:1274	exothermic	1265:1274	exothermic	1265:1274	Moreover, the formaldehyde uptake process by the aforementioned nanocomposite was exothermic and spontaneous.					
36417060	5	22	used	used	776:779	arg2	composite					762:770	Alginate@Fe3O4@Bentonite composite	737:770	Alginate@Fe3O4@Bentonite composite	737:770	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	3	23	theme	Bent	534:537	arg1	nanocomposite					539:551	Alg@Fe3O4@Bent nanocomposite	524:551	Alg@Fe3O4@Bent nanocomposite	524:551	The central composite design method was employed to find the optimal conditions for formaldehyde removal using Alg@Fe3O4@Bent nanocomposite.					
36417060	9	24	theme	Alginate	1420:1427	arg1	nanocomposite					1445:1457	Alginate@Fe3O4@Bentonite nanocomposite	1420:1457	Alginate@Fe3O4@Bentonite nanocomposite	1420:1457	Furthermore, the formaldehyde adsorption efficiency decreased slightly after six reuse cycles (less than 10%), indicating that Alginate@Fe3O4@Bentonite nanocomposite has remarkable recyclability.					
36417060	10	25	from	reduction	1753:1761	arg1	efficiency					1774:1783	removal efficiency	1766:1783	removal efficiency	1766:1783	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	0	26	theme	synthetic	69:77	arg1	effluent					88:95	synthetic and real effluent	69:95	synthetic and real effluent	69:95	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.					
36417060	9	27	theme	Fe3O4	1429:1433	arg1	nanocomposite					1445:1457	Alginate@Fe3O4@Bentonite nanocomposite	1420:1457	Alginate@Fe3O4@Bentonite nanocomposite	1420:1457	Furthermore, the formaldehyde adsorption efficiency decreased slightly after six reuse cycles (less than 10%), indicating that Alginate@Fe3O4@Bentonite nanocomposite has remarkable recyclability.					
36417060	8	28	theme	aforementioned	1232:1245	arg1	nanocomposite					1247:1259	the aforementioned nanocomposite	1228:1259	the aforementioned nanocomposite	1228:1259	Moreover, the formaldehyde uptake process by the aforementioned nanocomposite was exothermic and spontaneous.					
36417060	0	29	theme	real	83:86	arg1	effluent					88:95	synthetic and real effluent	69:95	synthetic and real effluent	69:95	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.					
36417060	2	30	theme	bentonite	285:293	arg1	Fe3O4					295:299	bentonite@Fe3O4	285:299	bentonite@Fe3O4	285:299	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	9	31	theme	Bentonite	1435:1443	arg1	nanocomposite					1445:1457	Alginate@Fe3O4@Bentonite nanocomposite	1420:1457	Alginate@Fe3O4@Bentonite nanocomposite	1420:1457	Furthermore, the formaldehyde adsorption efficiency decreased slightly after six reuse cycles (less than 10%), indicating that Alginate@Fe3O4@Bentonite nanocomposite has remarkable recyclability.					
36417060	2	32	theme	Mapping	366:372	arg1	analyses					403:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	5	33	from	wastewater	831:840	arg1	formaldehyde					794:805	formaldehyde	794:805	formaldehyde from Razi petrochemical wastewater	794:840	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	34	theme	formaldehyde	878:889	arg1	%					894:894	70%	892:894	70% of COD	892:901	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	34	theme	formaldehyde	878:889	arg1	%					912:912	68.9%	908:912	68.9% of BOD5	908:920	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	34	theme	formaldehyde	878:889	arg1	BOD5					917:920	BOD5	917:920	BOD5	917:920	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	34	theme	formaldehyde	878:889	arg1	%					873:873	91.24%	868:873	91.24% of formaldehyde	868:889	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	34	theme	formaldehyde	878:889	arg1	COD					899:901	COD	899:901	COD	899:901	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	34	theme	formaldehyde	878:889	arg1	formaldehyde					878:889	formaldehyde	878:889	formaldehyde	878:889	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	2	35	theme	VSM	390:392	arg1	analyses					403:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	2	36	theme	PXRD	360:363	arg1	analyses					403:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	0	37	theme	Alginate	0:7	arg1	nanocomposite					25:37	Alginate@Fe3O4@Bentonite nanocomposite	0:37	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.	0:138	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.					
36417060	2	38	theme	SEM	385:387	arg1	analyses					403:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	2	39	theme	Structural	251:260	arg1	features					262:269	Structural features	251:269	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite	251:329	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	2	40	theme	TEM	380:382	arg1	analyses					403:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	0	41	theme	Fe3O4	9:13	arg1	nanocomposite					25:37	Alginate@Fe3O4@Bentonite nanocomposite	0:37	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.	0:138	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.					
36417060	2	42	theme	EDX	375:377	arg1	analyses					403:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	0	43	theme	Bentonite	15:23	arg1	nanocomposite					25:37	Alginate@Fe3O4@Bentonite nanocomposite	0:37	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.	0:138	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.					
36417060	3	44	theme	central	417:423	arg1	method					442:447	The central composite design method	413:447	The central composite design method	413:447	The central composite design method was employed to find the optimal conditions for formaldehyde removal using Alg@Fe3O4@Bent nanocomposite.					
36417060	10	45	theme	removal	1766:1772	arg1	efficiency					1774:1783	removal efficiency	1766:1783	removal efficiency	1766:1783	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	2	46	theme	Fe3O4	315:319	arg1	Bentonite					321:329	Alginate@Fe3O4@Bentonite	306:329	Alginate@Fe3O4@Bentonite	306:329	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	10	47	theme	formaldehyde	1609:1620	arg1	efficiency					1630:1639	the formaldehyde removal efficiency	1605:1639	the formaldehyde removal efficiency	1605:1639	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	6	48	theme	formaldehyde	985:996	arg1	process					1005:1011	the formaldehyde uptake process	981:1011	the formaldehyde uptake process by the foresaid adsorbent	981:1037	The isotherm and kinetic investigations demonstrated that the formaldehyde uptake process by the foresaid adsorbent follows the Langmuir isotherm and quasi-first-order kinetic models, respectively.					
36417060	10	49	dep	reduction	1753:1761	arg1	%					1751:1751	%	1751:1751	%	1751:1751	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	9	50	contain	has	1459:1461	arg1	nanocomposite					1445:1457	Alginate@Fe3O4@Bentonite nanocomposite	1420:1457	Alginate@Fe3O4@Bentonite nanocomposite	1420:1457	Furthermore, the formaldehyde adsorption efficiency decreased slightly after six reuse cycles (less than 10%), indicating that Alginate@Fe3O4@Bentonite nanocomposite has remarkable recyclability.					
36417060	9	50	contain	has	1459:1461	arg2	recyclability					1474:1486	remarkable recyclability	1463:1486	remarkable recyclability	1463:1486	Furthermore, the formaldehyde adsorption efficiency decreased slightly after six reuse cycles (less than 10%), indicating that Alginate@Fe3O4@Bentonite nanocomposite has remarkable recyclability.					
36417060	2	51	theme	FT-IR	353:357	arg1	analyses					403:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	3	52	theme	design	435:440	arg1	method					442:447	The central composite design method	413:447	The central composite design method	413:447	The central composite design method was employed to find the optimal conditions for formaldehyde removal using Alg@Fe3O4@Bent nanocomposite.					
36417060	9	53	theme	adsorption	1323:1332	arg1	efficiency					1334:1343	the formaldehyde adsorption efficiency	1306:1343	the formaldehyde adsorption efficiency	1306:1343	Furthermore, the formaldehyde adsorption efficiency decreased slightly after six reuse cycles (less than 10%), indicating that Alginate@Fe3O4@Bentonite nanocomposite has remarkable recyclability.					
36417060	7	54	theme	maximum	1131:1137	arg1	capacity					1146:1153	the maximum uptake capacity	1127:1153	the maximum uptake capacity	1127:1153	Also, the maximum uptake capacity was obtained at 50.25 mg/g.					
36417060	2	55	theme	@	294:294	arg1	Fe3O4					295:299	bentonite@Fe3O4	285:299	bentonite@Fe3O4	285:299	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	3	56	theme	formaldehyde	497:508	arg1	removal					510:516	formaldehyde removal	497:516	formaldehyde removal using Alg@Fe3O4@Bent nanocomposite	497:551	The central composite design method was employed to find the optimal conditions for formaldehyde removal using Alg@Fe3O4@Bent nanocomposite.					
36417060	2	57	theme	Bentonite	321:329	arg1	features					262:269	Structural features	251:269	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite	251:329	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	9	58	theme	reuse	1374:1378	arg1	%					1400:1400	less than 10%	1388:1400	less than 10%	1388:1400	Furthermore, the formaldehyde adsorption efficiency decreased slightly after six reuse cycles (less than 10%), indicating that Alginate@Fe3O4@Bentonite nanocomposite has remarkable recyclability.					
36417060	9	58	theme	reuse	1374:1378	arg1	cycles					1380:1385	six reuse cycles	1370:1385	six reuse cycles (less than 10%)	1370:1401	Furthermore, the formaldehyde adsorption efficiency decreased slightly after six reuse cycles (less than 10%), indicating that Alginate@Fe3O4@Bentonite nanocomposite has remarkable recyclability.					
36417060	5	59	theme	Bentonite	752:760	arg1	composite					762:770	Alginate@Fe3O4@Bentonite composite	737:770	Alginate@Fe3O4@Bentonite composite	737:770	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	2	60	theme	Alginate	306:313	arg1	Bentonite					321:329	Alginate@Fe3O4@Bentonite	306:329	Alginate@Fe3O4@Bentonite	306:329	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	1	61	theme	@	163:163	arg1	nanocomposite					181:193	Alginate@ Fe3O4/Bentonite nanocomposite	155:193	Alginate@ Fe3O4/Bentonite nanocomposite	155:193	In this study, Alginate@ Fe3O4/Bentonite nanocomposite was utilized to eliminate formaldehyde from wastewater.					
36417060	10	62	dep	formaldehyde	1726:1737	arg1	reduction					1753:1761	less than 3% reduction	1740:1761	less than 3% reduction in removal efficiency	1740:1783	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	0	63	theme	central	114:120	arg1	design					132:137	central composite design	114:137	central composite design	114:137	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.					
36417060	5	64	theme	Alginate	737:744	arg1	composite					762:770	Alginate@Fe3O4@Bentonite composite	737:770	Alginate@Fe3O4@Bentonite composite	737:770	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	2	65	theme	@	320:320	arg1	Bentonite					321:329	Alginate@Fe3O4@Bentonite	306:329	Alginate@Fe3O4@Bentonite	306:329	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	3	66	theme	@	527:527	arg1	nanocomposite					539:551	Alg@Fe3O4@Bent nanocomposite	524:551	Alg@Fe3O4@Bent nanocomposite	524:551	The central composite design method was employed to find the optimal conditions for formaldehyde removal using Alg@Fe3O4@Bent nanocomposite.					
36417060	5	67	theme	Fe3O4	746:750	arg1	composite					762:770	Alginate@Fe3O4@Bentonite composite	737:770	Alginate@Fe3O4@Bentonite composite	737:770	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	1	68	used	utilized	199:206	arg2	nanocomposite					181:193	Alginate@ Fe3O4/Bentonite nanocomposite	155:193	Alginate@ Fe3O4/Bentonite nanocomposite	155:193	In this study, Alginate@ Fe3O4/Bentonite nanocomposite was utilized to eliminate formaldehyde from wastewater.					
36417060	4	69	theme	formaldehyde	566:577	arg1	efficiency					586:595	The maximum formaldehyde uptake efficiency	554:595	The maximum formaldehyde uptake efficiency (94.56%)	554:604	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	4	69	theme	formaldehyde	566:577	arg1	%					603:603	94.56%	598:603	94.56%	598:603	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	10	70	theme	ions	1527:1530	arg1	influence					1502:1510	the influence	1498:1510	the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate	1498:1588	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	5	71	theme	Razi	812:815	arg1	wastewater					831:840	Razi petrochemical wastewater	812:840	Razi petrochemical wastewater	812:840	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	4	72	theme	formaldehyde	622:633	arg1	concentration					635:647	formaldehyde concentration	622:647	formaldehyde concentration of 10.69 ppm	622:660	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	10	73	contain	have	1700:1703	arg1	ions					1676:1679	all ions	1672:1679	all ions except nitrate ion	1672:1698	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	10	73	contain	have	1700:1703	arg2	interaction					1709:1719	low interaction	1705:1719	low interaction with formaldehyde (less than 3% reduction in removal efficiency)	1705:1784	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	0	74	from	effluent	88:95	arg1	removal					56:62	formaldehyde removal	43:62	formaldehyde removal from synthetic and real effluent	43:95	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.					
36417060	4	75	theme	1.28 g/L	689:696	arg1	concentration					635:647	formaldehyde concentration	622:647	formaldehyde concentration of 10.69 ppm	622:660	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	4	75	theme	1.28 g/L	689:696	arg1	pH					703:704	pH	703:704	pH of 9.96	703:712	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	4	75	theme	1.28 g/L	689:696	arg1	dose					681:684	the nanocomposite dose	663:684	the nanocomposite dose of 1.28 g/L	663:696	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	3	76	theme	@	533:533	arg1	nanocomposite					539:551	Alg@Fe3O4@Bent nanocomposite	524:551	Alg@Fe3O4@Bent nanocomposite	524:551	The central composite design method was employed to find the optimal conditions for formaldehyde removal using Alg@Fe3O4@Bent nanocomposite.					
36417060	6	77	theme	quasi-first-order	1073:1089	arg1	models					1099:1104	quasi-first-order kinetic models	1073:1104	quasi-first-order kinetic models	1073:1104	The isotherm and kinetic investigations demonstrated that the formaldehyde uptake process by the foresaid adsorbent follows the Langmuir isotherm and quasi-first-order kinetic models, respectively.					
36417060	8	78	theme	formaldehyde	1197:1208	arg1	process					1217:1223	the formaldehyde uptake process	1193:1223	the formaldehyde uptake process by the aforementioned nanocomposite	1193:1259	Moreover, the formaldehyde uptake process by the aforementioned nanocomposite was exothermic and spontaneous.					
36417060	8	78	theme	formaldehyde	1197:1208	arg1	exothermic					1265:1274	exothermic	1265:1274	exothermic	1265:1274	Moreover, the formaldehyde uptake process by the aforementioned nanocomposite was exothermic and spontaneous.					
36417060	9	79	theme	@	1428:1428	arg1	nanocomposite					1445:1457	Alginate@Fe3O4@Bentonite nanocomposite	1420:1457	Alginate@Fe3O4@Bentonite nanocomposite	1420:1457	Furthermore, the formaldehyde adsorption efficiency decreased slightly after six reuse cycles (less than 10%), indicating that Alginate@Fe3O4@Bentonite nanocomposite has remarkable recyclability.					
36417060	9	80	theme	@	1434:1434	arg1	nanocomposite					1445:1457	Alginate@Fe3O4@Bentonite nanocomposite	1420:1457	Alginate@Fe3O4@Bentonite nanocomposite	1420:1457	Furthermore, the formaldehyde adsorption efficiency decreased slightly after six reuse cycles (less than 10%), indicating that Alginate@Fe3O4@Bentonite nanocomposite has remarkable recyclability.					
36417060	6	81	theme	kinetic	940:946	arg1	investigations					948:961	The isotherm and kinetic investigations	923:961	investigations	948:961	The isotherm and kinetic investigations demonstrated that the formaldehyde uptake process by the foresaid adsorbent follows the Langmuir isotherm and quasi-first-order kinetic models, respectively.					
36417060	0	82	theme	@	8:8	arg1	nanocomposite					25:37	Alginate@Fe3O4@Bentonite nanocomposite	0:37	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.	0:138	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.					
36417060	9	83	theme	remarkable	1463:1472	arg1	recyclability					1474:1486	remarkable recyclability	1463:1486	remarkable recyclability	1463:1486	Furthermore, the formaldehyde adsorption efficiency decreased slightly after six reuse cycles (less than 10%), indicating that Alginate@Fe3O4@Bentonite nanocomposite has remarkable recyclability.					
36417060	6	84	theme	isotherm	927:934	arg1	investigations					948:961	The isotherm and kinetic investigations	923:961	investigations	948:961	The isotherm and kinetic investigations demonstrated that the formaldehyde uptake process by the foresaid adsorbent follows the Langmuir isotherm and quasi-first-order kinetic models, respectively.					
36417060	0	85	theme	@	14:14	arg1	nanocomposite					25:37	Alginate@Fe3O4@Bentonite nanocomposite	0:37	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.	0:138	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.					
36417060	10	86	theme	low	1705:1707	arg1	interaction					1709:1719	low interaction	1705:1719	low interaction with formaldehyde (less than 3% reduction in removal efficiency)	1705:1784	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	4	87	theme	nanocomposite	667:679	arg1	dose					681:684	the nanocomposite dose	663:684	the nanocomposite dose of 1.28 g/L	663:696	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	5	88	theme	COD	899:901	arg1	%					894:894	70%	892:894	70% of COD	892:901	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	88	theme	COD	899:901	arg1	%					912:912	68.9%	908:912	68.9% of BOD5	908:920	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	88	theme	COD	899:901	arg1	BOD5					917:920	BOD5	917:920	BOD5	917:920	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	88	theme	COD	899:901	arg1	%					873:873	91.24%	868:873	91.24% of formaldehyde	868:889	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	88	theme	COD	899:901	arg1	COD					899:901	COD	899:901	COD	899:901	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	5	88	theme	COD	899:901	arg1	formaldehyde					878:889	formaldehyde	878:889	formaldehyde	878:889	Also, Alginate@Fe3O4@Bentonite composite was used to eliminate formaldehyde from Razi petrochemical wastewater and was able to eliminate 91.24% of formaldehyde, 70% of COD, and 68.9% of BOD5.					
36417060	2	89	theme	BET	399:401	arg1	analyses					403:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses	353:410	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	0	90	theme	formaldehyde	43:54	arg1	removal					56:62	formaldehyde removal	43:62	formaldehyde removal from synthetic and real effluent	43:95	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.					
36417060	0	91	dep	nanocomposite	25:37	arg1	optimization					98:109	optimization	98:109	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.	0:138	Alginate@Fe3O4@Bentonite nanocomposite for formaldehyde removal from synthetic and real effluent: optimization by central composite design.					
36417060	3	92	theme	composite	425:433	arg1	method					442:447	The central composite design method	413:447	The central composite design method	413:447	The central composite design method was employed to find the optimal conditions for formaldehyde removal using Alg@Fe3O4@Bent nanocomposite.					
36417060	6	93	theme	uptake	998:1003	arg1	process					1005:1011	the formaldehyde uptake process	981:1011	the formaldehyde uptake process by the foresaid adsorbent	981:1037	The isotherm and kinetic investigations demonstrated that the formaldehyde uptake process by the foresaid adsorbent follows the Langmuir isotherm and quasi-first-order kinetic models, respectively.					
36417060	2	94	theme	@	314:314	arg1	Bentonite					321:329	Alginate@Fe3O4@Bentonite	306:329	Alginate@Fe3O4@Bentonite	306:329	Structural features of bentonite, bentonite@Fe3O4, and Alginate@Fe3O4@Bentonite were determined using FT-IR, PXRD, Mapping, EDX, TEM, SEM, VSM, and BET analyses.					
36417060	7	95	theme	uptake	1139:1144	arg1	capacity					1146:1153	the maximum uptake capacity	1127:1153	the maximum uptake capacity	1127:1153	Also, the maximum uptake capacity was obtained at 50.25 mg/g.					
36417060	4	96	theme	9.96	709:712	arg1	concentration					635:647	formaldehyde concentration	622:647	formaldehyde concentration of 10.69 ppm	622:660	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	4	96	theme	9.96	709:712	arg1	pH					703:704	pH	703:704	pH of 9.96	703:712	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	4	96	theme	9.96	709:712	arg1	dose					681:684	the nanocomposite dose	663:684	the nanocomposite dose of 1.28 g/L	663:696	The maximum formaldehyde uptake efficiency (94.56%) was obtained at formaldehyde concentration of 10.69 ppm, the nanocomposite dose of 1.28 g/L, and pH of 9.96 after 16.53 min.					
36417060	10	97	theme	removal	1622:1628	arg1	efficiency					1630:1639	the formaldehyde removal efficiency	1605:1639	the formaldehyde removal efficiency	1605:1639	Besides, the influence of interfering ions like nitrate, carbonate, chloride, phosphate, and sulfate was studied on the formaldehyde removal efficiency and the results displayed that all ions except nitrate ion have low interaction with formaldehyde (less than 3% reduction in removal efficiency).					
36417060	9	98	theme	formaldehyde	1310:1321	arg1	efficiency					1334:1343	the formaldehyde adsorption efficiency	1306:1343	the formaldehyde adsorption efficiency	1306:1343	Furthermore, the formaldehyde adsorption efficiency decreased slightly after six reuse cycles (less than 10%), indicating that Alginate@Fe3O4@Bentonite nanocomposite has remarkable recyclability.					
35399669	0	0	theme	Molecular	81:89	arg1	Polysaccharide					98:111	a High Molecular Weight Polysaccharide	74:111	a High Molecular Weight Polysaccharide	74:111	Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From Ganoderma lucidum.					
35399669	3	1	theme	[high	346:350	arg1	Polymorphism					383:394	[high Mw Restriction Fragment Length Polymorphism	346:394	[high Mw Restriction Fragment Length Polymorphism (RGLP)	346:401	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	1	theme	[high	346:350	arg1	fractions					336:344	two fractions	332:344	two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP]	332:418	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	1	theme	[high	346:350	arg1	RGLP					397:400	RGLP	397:400	RGLP	397:400	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	7	2	theme	molar	1006:1010	arg1	ratio					1012:1016	a molar ratio	1004:1016	a molar ratio of 40.08: 8.11: 5.62: 17.81	1004:1044	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	8	3	dep	intertwined	1149:1159	arg1	RGLP-1					1142:1147	RGLP-1	1142:1147	RGLP-1	1142:1147	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	8	4	theme	electron	1056:1063	arg1	microscopy					1065:1074	Scanning electron microscopy	1047:1074	Scanning electron microscopy	1047:1074	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	0	5	theme	High	76:79	arg1	Polysaccharide					98:111	a High Molecular Weight Polysaccharide	74:111	a High Molecular Weight Polysaccharide	74:111	Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From Ganoderma lucidum.					
35399669	4	6	theme	immunomodulatory	513:528	arg1	higher					565:570	higher	565:570	higher	565:570	Although the RGLP content was low in GLP, the immunomodulatory activity in RGLP was significantly higher than that of EGLP.					
35399669	4	6	theme	immunomodulatory	513:528	arg1	activity					530:537	the immunomodulatory activity	509:537	the immunomodulatory activity in RGLP	509:545	Although the RGLP content was low in GLP, the immunomodulatory activity in RGLP was significantly higher than that of EGLP.					
35399669	8	7	from	conformation	1262:1273	arg1	water					1278:1282	water	1278:1282	water	1278:1282	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	8	8	with	structure	1234:1242	arg1	conformation					1262:1273	triple-helix conformation	1249:1273	triple-helix conformation in water	1249:1282	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	7	9	theme	methylation	878:888	arg1	results					890:896	Periodate oxidation, Smith degradation, and methylation results	834:896	Periodate oxidation, Smith degradation, and methylation results	834:896	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	0	10	theme	Polysaccharide	98:111	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From Ganoderma lucidum.					
35399669	0	10	theme	Polysaccharide	98:111	arg1	Characterization					23:38	Structural Characterization	12:38	Structural Characterization	12:38	Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From Ganoderma lucidum.					
35399669	0	10	theme	Polysaccharide	98:111	arg1	Activity					62:69	Immunomodulatory Activity	45:69	Immunomodulatory Activity	45:69	Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From Ganoderma lucidum.					
35399669	0	11	theme	Weight	91:96	arg1	Polysaccharide					98:111	a High Molecular Weight Polysaccharide	74:111	a High Molecular Weight Polysaccharide	74:111	Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From Ganoderma lucidum.					
35399669	4	12	from	GLP	504:506	arg1	low					497:499	low	497:499	low	497:499	Although the RGLP content was low in GLP, the immunomodulatory activity in RGLP was significantly higher than that of EGLP.					
35399669	4	12	from	GLP	504:506	arg1	content					485:491	the RGLP content	476:491	the RGLP content	476:491	Although the RGLP content was low in GLP, the immunomodulatory activity in RGLP was significantly higher than that of EGLP.					
35399669	8	13	theme	triple-helix	1249:1260	arg1	conformation					1262:1273	triple-helix conformation	1249:1273	triple-helix conformation in water	1249:1282	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	3	14	theme	Length	376:381	arg1	Polymorphism					383:394	[high Mw Restriction Fragment Length Polymorphism	346:394	[high Mw Restriction Fragment Length Polymorphism (RGLP)	346:401	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	14	theme	Length	376:381	arg1	fractions					336:344	two fractions	332:344	two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP]	332:418	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	14	theme	Length	376:381	arg1	RGLP					397:400	RGLP	397:400	RGLP	397:400	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	8	15	theme	force	1084:1088	arg1	microscopy					1090:1099	atomic force microscopy	1077:1099	atomic force microscopy	1077:1099	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	3	16	theme	kDa	429:431	arg1	membrane					457:464	10 kDa cut-off ultrafiltration membrane	426:464	10 kDa cut-off ultrafiltration membrane	426:464	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	17	theme	ultrafiltration	441:455	arg1	membrane					457:464	10 kDa cut-off ultrafiltration membrane	426:464	10 kDa cut-off ultrafiltration membrane	426:464	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	18	dep	fractions	336:344	arg1	Polymorphism					383:394	[high Mw Restriction Fragment Length Polymorphism	346:394	[high Mw Restriction Fragment Length Polymorphism (RGLP)	346:401	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	18	dep	fractions	336:344	arg1	fractions					336:344	two fractions	332:344	two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP]	332:418	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	18	dep	fractions	336:344	arg1	EGLP					414:417	low Mw EGLP	407:417	low Mw EGLP	407:417	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	18	dep	fractions	336:344	arg1	RGLP					397:400	RGLP	397:400	RGLP	397:400	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	4	19	from	low	497:499	arg1	GLP					504:506	GLP	504:506	GLP	504:506	Although the RGLP content was low in GLP, the immunomodulatory activity in RGLP was significantly higher than that of EGLP.					
35399669	7	20	theme	Smith	855:859	arg1	degradation					861:871	Smith degradation	855:871	Smith degradation	855:871	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	1	21	theme	excellent	187:195	arg1	activity					214:221	excellent immunomodulatory activity	187:221	excellent immunomodulatory activity	187:221	Ganoderma lucidum polysaccharides (GLP) exhibited excellent immunomodulatory activity.					
35399669	3	22	theme	low	407:409	arg1	EGLP					414:417	low Mw EGLP	407:417	low Mw EGLP	407:417	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	22	theme	low	407:409	arg1	fractions					336:344	two fractions	332:344	two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP]	332:418	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	23	theme	Mw	352:353	arg1	Polymorphism					383:394	[high Mw Restriction Fragment Length Polymorphism	346:394	[high Mw Restriction Fragment Length Polymorphism (RGLP)	346:401	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	23	theme	Mw	352:353	arg1	fractions					336:344	two fractions	332:344	two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP]	332:418	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	23	theme	Mw	352:353	arg1	RGLP					397:400	RGLP	397:400	RGLP	397:400	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	7	24	dep	40.08	1021:1025	arg1	17.81					1040:1044	17.81	1040:1044	17.81	1040:1044	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	7	24	dep	40.08	1021:1025	arg1	5.62					1034:1037	5.62	1034:1037	5.62	1034:1037	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	7	24	dep	40.08	1021:1025	arg1	8.11					1028:1031	8.11	1028:1031	8.11	1028:1031	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	7	25	theme	degradation	861:871	arg1	results					890:896	Periodate oxidation, Smith degradation, and methylation results	834:896	Periodate oxidation, Smith degradation, and methylation results	834:896	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	1	26	theme	immunomodulatory	197:212	arg1	activity					214:221	excellent immunomodulatory activity	187:221	excellent immunomodulatory activity	187:221	Ganoderma lucidum polysaccharides (GLP) exhibited excellent immunomodulatory activity.					
35399669	3	27	theme	Mw	411:412	arg1	EGLP					414:417	low Mw EGLP	407:417	low Mw EGLP	407:417	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	27	theme	Mw	411:412	arg1	fractions					336:344	two fractions	332:344	two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP]	332:418	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	7	28	with	polysaccharide	933:946	arg1	1→3					979:981	1→3	979:981	1→3	979:981	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	7	28	with	polysaccharide	933:946	arg1	1→4					965:967	1→4	965:967	1→4	965:967	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	7	28	with	polysaccharide	933:946	arg1	1→3					960:962	1→3	960:962	1→3	960:962	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	7	28	with	polysaccharide	933:946	arg1	bonds					995:999	6 glycosyl bonds	984:999	6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81	984:1044	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	7	28	with	polysaccharide	933:946	arg1	1→6					970:972	1→6	970:972	1→6	970:972	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	5	29	theme	Sephacryl	636:644	arg1	column					646:651	the Sephacryl column	632:651	the Sephacryl column	632:651	Moreover, RGLP was further separated via the Sephacryl column to obtain RGLP-1 showed the best immunomodulatory activity in the macrophage RAW264.7 model.					
35399669	6	30	theme	3,978	791:795	arg1	kDa					797:799	kDa	797:799	kDa	797:799	Structural analysis revealed that RGLP-1 was 3,978 kDa and mainly consisted of glucose.					
35399669	7	31	theme	oxidation	844:852	arg1	results					890:896	Periodate oxidation, Smith degradation, and methylation results	834:896	Periodate oxidation, Smith degradation, and methylation results	834:896	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	3	32	theme	cut-off	433:439	arg1	membrane					457:464	10 kDa cut-off ultrafiltration membrane	426:464	10 kDa cut-off ultrafiltration membrane	426:464	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	0	33	theme	Structural	12:21	arg1	Characterization					23:38	Structural Characterization	12:38	Structural Characterization	12:38	Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From Ganoderma lucidum.					
35399669	8	34	dep	other	1171:1175	arg1	possess					1215:1221	possess	1215:1221	might possess a globular structure with triple-helix conformation in water	1209:1282	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	8	34	dep	other	1171:1175	arg1	form					1180:1183	form	1180:1183	to form circular aggregates	1177:1203	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	2	35	dep	structure	243:251	arg1	the					239:241	the	239:241	the	239:241	Unfortunately, the structure and immunomodulatory activity of GLP are still unclear.					
35399669	7	36	theme	40.08	1021:1025	arg1	ratio					1012:1016	a molar ratio	1004:1016	a molar ratio of 40.08: 8.11: 5.62: 17.81	1004:1044	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	0	37	theme	Ganoderma	118:126	arg1	lucidum					128:134	Ganoderma lucidum	118:134	Ganoderma lucidum	118:134	Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From Ganoderma lucidum.					
35399669	7	38	theme	Periodate	834:842	arg1	oxidation					844:852	Periodate oxidation	834:852	Periodate oxidation	834:852	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	8	39	theme	globular	1225:1232	arg1	structure					1234:1242	a globular structure	1223:1242	a globular structure with triple-helix conformation in water	1223:1282	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	3	40	theme	10	426:427	arg1	kDa					429:431	kDa	429:431	kDa	429:431	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	5	41	theme	macrophage	719:728	arg1	model					739:743	the macrophage RAW264.7 model	715:743	the macrophage RAW264.7 model	715:743	Moreover, RGLP was further separated via the Sephacryl column to obtain RGLP-1 showed the best immunomodulatory activity in the macrophage RAW264.7 model.					
35399669	4	42	theme	RGLP	480:483	arg1	low					497:499	low	497:499	low	497:499	Although the RGLP content was low in GLP, the immunomodulatory activity in RGLP was significantly higher than that of EGLP.					
35399669	4	42	theme	RGLP	480:483	arg1	content					485:491	the RGLP content	476:491	the RGLP content	476:491	Although the RGLP content was low in GLP, the immunomodulatory activity in RGLP was significantly higher than that of EGLP.					
35399669	2	43	theme	immunomodulatory	257:272	arg1	activity					274:281	immunomodulatory activity	257:281	immunomodulatory activity	257:281	Unfortunately, the structure and immunomodulatory activity of GLP are still unclear.					
35399669	8	44	with	intertwined	1149:1159	arg1	other					1171:1175	other	1171:1175	other	1171:1175	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	8	45	theme	red	1112:1114	arg1	experiments					1116:1126	Congo red experiments	1106:1126	Congo red experiments	1106:1126	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	5	46	theme	RAW264.7	730:737	arg1	model					739:743	the macrophage RAW264.7 model	715:743	the macrophage RAW264.7 model	715:743	Moreover, RGLP was further separated via the Sephacryl column to obtain RGLP-1 showed the best immunomodulatory activity in the macrophage RAW264.7 model.					
35399669	4	47	from	activity	530:537	arg1	RGLP					542:545	RGLP	542:545	RGLP	542:545	Although the RGLP content was low in GLP, the immunomodulatory activity in RGLP was significantly higher than that of EGLP.					
35399669	8	48	theme	atomic	1077:1082	arg1	microscopy					1090:1099	atomic force microscopy	1077:1099	atomic force microscopy	1077:1099	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	0	49	theme	Immunomodulatory	45:60	arg1	Activity					62:69	Immunomodulatory Activity	45:69	Immunomodulatory Activity	45:69	Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From Ganoderma lucidum.					
35399669	8	50	theme	Scanning	1047:1054	arg1	microscopy					1065:1074	Scanning electron microscopy	1047:1074	Scanning electron microscopy	1047:1074	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	9	51	theme	potential	1328:1336	arg1	ingredient					1354:1363	a potential functional food ingredient	1326:1363	a potential functional food ingredient	1326:1363	Overall, these results provide RGLP-1 as a potential functional food ingredient or pharmaceutical for immunomodulatory.					
35399669	5	52	theme	best	681:684	arg1	activity					703:710	the best immunomodulatory activity	677:710	the best immunomodulatory activity	677:710	Moreover, RGLP was further separated via the Sephacryl column to obtain RGLP-1 showed the best immunomodulatory activity in the macrophage RAW264.7 model.					
35399669	7	53	theme	glycosyl	986:993	arg1	1→3					979:981	1→3	979:981	1→3	979:981	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	7	53	theme	glycosyl	986:993	arg1	bonds					995:999	6 glycosyl bonds	984:999	6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81	984:1044	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	1	54	theme	Ganoderma	137:145	arg1	lucidum					147:153	Ganoderma lucidum	137:153	Ganoderma lucidum polysaccharides (GLP)	137:175	Ganoderma lucidum polysaccharides (GLP) exhibited excellent immunomodulatory activity.					
35399669	7	55	theme	β-pyran	925:931	arg1	polysaccharide					933:946	a β-pyran polysaccharide	923:946	a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81	923:1044	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	7	55	theme	β-pyran	925:931	arg1	RGLP-1					913:918	RGLP-1	913:918	RGLP-1	913:918	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	9	56	theme	functional	1338:1347	arg1	ingredient					1354:1363	a potential functional food ingredient	1326:1363	a potential functional food ingredient	1326:1363	Overall, these results provide RGLP-1 as a potential functional food ingredient or pharmaceutical for immunomodulatory.					
35399669	5	57	theme	immunomodulatory	686:701	arg1	activity					703:710	the best immunomodulatory activity	677:710	the best immunomodulatory activity	677:710	Moreover, RGLP was further separated via the Sephacryl column to obtain RGLP-1 showed the best immunomodulatory activity in the macrophage RAW264.7 model.					
35399669	6	58	theme	Structural	746:755	arg1	analysis					757:764	Structural analysis	746:764	Structural analysis	746:764	Structural analysis revealed that RGLP-1 was 3,978 kDa and mainly consisted of glucose.					
35399669	1	59	theme	lucidum	147:153	arg1	GLP					172:174	GLP	172:174	GLP	172:174	Ganoderma lucidum polysaccharides (GLP) exhibited excellent immunomodulatory activity.					
35399669	1	59	theme	lucidum	147:153	arg1	polysaccharides					155:169	Ganoderma lucidum polysaccharides	137:169	Ganoderma lucidum polysaccharides (GLP)	137:175	Ganoderma lucidum polysaccharides (GLP) exhibited excellent immunomodulatory activity.					
35399669	9	60	theme	food	1349:1352	arg1	ingredient					1354:1363	a potential functional food ingredient	1326:1363	a potential functional food ingredient	1326:1363	Overall, these results provide RGLP-1 as a potential functional food ingredient or pharmaceutical for immunomodulatory.					
35399669	3	61	theme	Restriction	355:365	arg1	Polymorphism					383:394	[high Mw Restriction Fragment Length Polymorphism	346:394	[high Mw Restriction Fragment Length Polymorphism (RGLP)	346:401	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	61	theme	Restriction	355:365	arg1	fractions					336:344	two fractions	332:344	two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP]	332:418	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	61	theme	Restriction	355:365	arg1	RGLP					397:400	RGLP	397:400	RGLP	397:400	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	2	62	theme	GLP	286:288	arg1	structure					243:251	structure	243:251	structure	243:251	Unfortunately, the structure and immunomodulatory activity of GLP are still unclear.					
35399669	2	62	theme	GLP	286:288	arg1	activity					274:281	immunomodulatory activity	257:281	immunomodulatory activity	257:281	Unfortunately, the structure and immunomodulatory activity of GLP are still unclear.					
35399669	7	63	from	ratio	1012:1016	arg1	1→3					979:981	1→3	979:981	1→3	979:981	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	7	63	from	ratio	1012:1016	arg1	bonds					995:999	6 glycosyl bonds	984:999	6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81	984:1044	Periodate oxidation, Smith degradation, and methylation results indicated that RGLP-1 is a β-pyran polysaccharide mainly with 1→3, 1→4, 1→6, and 1→3, 6 glycosyl bonds at a molar ratio of 40.08: 8.11: 5.62: 17.81.					
35399669	3	64	theme	Fragment	367:374	arg1	Polymorphism					383:394	[high Mw Restriction Fragment Length Polymorphism	346:394	[high Mw Restriction Fragment Length Polymorphism (RGLP)	346:401	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	64	theme	Fragment	367:374	arg1	fractions					336:344	two fractions	332:344	two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP]	332:418	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	3	64	theme	Fragment	367:374	arg1	RGLP					397:400	RGLP	397:400	RGLP	397:400	GLP was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) and low Mw EGLP] using 10 kDa cut-off ultrafiltration membrane.					
35399669	8	65	dep	revealed	1128:1135	arg1	intertwined					1149:1159	intertwined	1149:1159	intertwined	1149:1159	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	8	66	theme	circular	1185:1192	arg1	aggregates					1194:1203	circular aggregates	1185:1203	circular aggregates	1185:1203	Scanning electron microscopy, atomic force microscopy, and Congo red experiments revealed that RGLP-1 intertwined with each other to form circular aggregates and might possess a globular structure with triple-helix conformation in water.					
35399669	0	67	from	lucidum	128:134	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From Ganoderma lucidum.					
35399669	0	67	from	lucidum	128:134	arg1	Characterization					23:38	Structural Characterization	12:38	Structural Characterization	12:38	Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From Ganoderma lucidum.					
35399669	0	67	from	lucidum	128:134	arg1	Activity					62:69	Immunomodulatory Activity	45:69	Immunomodulatory Activity	45:69	Extraction, Structural Characterization, and Immunomodulatory Activity of a High Molecular Weight Polysaccharide From Ganoderma lucidum.					
36269217	5	0	theme	1-year	1078:1083	arg1	incubations					1093:1103	1-year in situ incubations	1078:1103	1-year in situ incubations	1078:1103	Poly-l-lactic acid showed no signs of degradation, whereas poly(3-hydroxybutyrate/3-hydroxyvalerate) (PHB/HV), plasticized starch (PR), and cellulose acetate (CA) degraded completely or almost completely during 1-year in situ incubations.					
36269217	0	1	theme	Bioplastic	80:89	arg1	Materials					91:99	Commonly Used Bioplastic Materials	66:99	Commonly Used Bioplastic Materials	66:99	Degradation Rates and Bacterial Community Compositions Vary among Commonly Used Bioplastic Materials in a Brackish Marine Environment.					
36269217	6	2	theme	carbon	1173:1178	arg1	substrates					1180:1189	complex carbon substrates	1165:1189	complex carbon substrates	1165:1189	In accordance, bacterial taxa potentially capable of using complex carbon substrates and belonging, e.g., to class Gammaproteobacteria were significantly enriched on PHB/HV, PR, and CA.					
36269217	3	3	dep	attrition	483:491	arg1	the					472:474	the	472:474	the	472:474	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	8	4	from	differences	1420:1430	arg1	rates					1470:1474	the persistence and biodegradation rates	1435:1474	the persistence and biodegradation rates among bioplastics	1435:1492	The results show substantial differences in the persistence and biodegradation rates among bioplastics, thus highlighting the need for carefully selecting materials for applications with risk of becoming marine litter.					
36269217	5	5	theme	cellulose	1007:1015	arg1	acetate					1017:1023	cellulose acetate	1007:1023	cellulose acetate (CA)	1007:1028	Poly-l-lactic acid showed no signs of degradation, whereas poly(3-hydroxybutyrate/3-hydroxyvalerate) (PHB/HV), plasticized starch (PR), and cellulose acetate (CA) degraded completely or almost completely during 1-year in situ incubations.					
36269217	5	5	theme	cellulose	1007:1015	arg1	CA					1026:1027	CA	1026:1027	CA	1026:1027	Poly-l-lactic acid showed no signs of degradation, whereas poly(3-hydroxybutyrate/3-hydroxyvalerate) (PHB/HV), plasticized starch (PR), and cellulose acetate (CA) degraded completely or almost completely during 1-year in situ incubations.					
36269217	3	6	theme	conventional	569:580	arg1	polyethylene					592:603	conventional oil-based polyethylene	569:603	conventional oil-based polyethylene	569:603	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	2	7	theme	litter	299:304	arg1	accumulation					283:294	the accumulation	279:294	the accumulation of litter in the environment	279:323	As a result of the pressures of replacing oil-based materials and reducing the accumulation of litter in the environment, the use of bioplastics is increasing, despite little being known about their accurate biodegradation in natural conditions.					
36269217	0	8	theme	Marine	115:120	arg1	Environment					122:132	a Brackish Marine Environment	104:132	a Brackish Marine Environment	104:132	Degradation Rates and Bacterial Community Compositions Vary among Commonly Used Bioplastic Materials in a Brackish Marine Environment.					
36269217	7	9	theme	gammaproteobacterial	1307:1326	arg1	abundance					1328:1336	gammaproteobacterial abundance	1307:1336	gammaproteobacterial abundance	1307:1336	An increase in gammaproteobacterial abundance was also observed in the biodegradation experiments.					
36269217	6	10	theme	bacterial	1121:1129	arg1	taxa					1131:1134	bacterial taxa	1121:1134	bacterial taxa potentially capable of using complex carbon substrates and belonging, e.g., to class Gammaproteobacteria	1121:1239	In accordance, bacterial taxa potentially capable of using complex carbon substrates and belonging, e.g., to class Gammaproteobacteria were significantly enriched on PHB/HV, PR, and CA.					
36269217	3	11	theme	materials	547:555	arg1	attrition					483:491	weight attrition	476:491	weight attrition	476:491	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	3	11	theme	materials	547:555	arg1	behavior					509:516	degradation behavior	497:516	degradation behavior	497:516	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	1	12	theme	aquatic	184:190	arg1	ecosystems					192:201	aquatic ecosystems	184:201	aquatic ecosystems	184:201	Plastic pollution threatens both terrestrial and aquatic ecosystems.					
36269217	0	13	theme	Brackish	106:113	arg1	Environment					122:132	a Brackish Marine Environment	104:132	a Brackish Marine Environment	104:132	Degradation Rates and Bacterial Community Compositions Vary among Commonly Used Bioplastic Materials in a Brackish Marine Environment.					
36269217	7	14	from	increase	1295:1302	arg1	abundance					1328:1336	gammaproteobacterial abundance	1307:1336	gammaproteobacterial abundance	1307:1336	An increase in gammaproteobacterial abundance was also observed in the biodegradation experiments.					
36269217	3	15	dep	in	621:622	arg1	situ					624:627	situ	624:627	situ	624:627	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	5	16	theme	Poly-l-lactic	867:879	arg1	acid					881:884	Poly-l-lactic acid	867:884	Poly-l-lactic acid	867:884	Poly-l-lactic acid showed no signs of degradation, whereas poly(3-hydroxybutyrate/3-hydroxyvalerate) (PHB/HV), plasticized starch (PR), and cellulose acetate (CA) degraded completely or almost completely during 1-year in situ incubations.					
36269217	3	17	from	experiments	708:718	arg1	laboratory					727:736	the laboratory	723:736	the laboratory	723:736	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	8	18	theme	marine	1595:1600	arg1	litter					1602:1607	marine litter	1595:1607	marine litter	1595:1607	The results show substantial differences in the persistence and biodegradation rates among bioplastics, thus highlighting the need for carefully selecting materials for applications with risk of becoming marine litter.					
36269217	0	19	theme	Degradation	0:10	arg1	Rates					12:16	Degradation Rates	0:16	Degradation Rates	0:16	Degradation Rates and Bacterial Community Compositions Vary among Commonly Used Bioplastic Materials in a Brackish Marine Environment.					
36269217	5	20	theme	in	1085:1086	arg1	incubations					1093:1103	1-year in situ incubations	1078:1103	1-year in situ incubations	1078:1103	Poly-l-lactic acid showed no signs of degradation, whereas poly(3-hydroxybutyrate/3-hydroxyvalerate) (PHB/HV), plasticized starch (PR), and cellulose acetate (CA) degraded completely or almost completely during 1-year in situ incubations.					
36269217	4	21	theme	plastic-degrading	811:827	arg1	bacteria					829:836	putative plastic-degrading bacteria	802:836	putative plastic-degrading bacteria	802:836	Bacterial communities were also investigated to verify whether putative plastic-degrading bacteria are enriched on bioplastics.					
36269217	7	22	located	observed	1347:1354	arg1	experiments					1378:1388	the biodegradation experiments	1359:1388	the biodegradation experiments	1359:1388	An increase in gammaproteobacterial abundance was also observed in the biodegradation experiments.					
36269217	7	22	located	observed	1347:1354	arg2	increase					1295:1302	An increase	1292:1302	An increase in gammaproteobacterial abundance	1292:1336	An increase in gammaproteobacterial abundance was also observed in the biodegradation experiments.					
36269217	0	23	theme	Bacterial	22:30	arg1	Compositions					42:53	Bacterial Community Compositions	22:53	Bacterial Community Compositions	22:53	Degradation Rates and Bacterial Community Compositions Vary among Commonly Used Bioplastic Materials in a Brackish Marine Environment.					
36269217	3	24	theme	in	621:622	arg1	incubation					629:638	a 1-year in situ incubation	612:638	a 1-year in situ incubation in the brackish Baltic Sea	612:665	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	3	25	theme	oil-based	582:590	arg1	polyethylene					592:603	conventional oil-based polyethylene	569:603	conventional oil-based polyethylene	569:603	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	8	26	theme	biodegradation	1455:1468	arg1	rates					1470:1474	the persistence and biodegradation rates	1435:1474	the persistence and biodegradation rates among bioplastics	1435:1492	The results show substantial differences in the persistence and biodegradation rates among bioplastics, thus highlighting the need for carefully selecting materials for applications with risk of becoming marine litter.					
36269217	2	27	theme	natural	430:436	arg1	conditions					438:447	natural conditions	430:447	natural conditions	430:447	As a result of the pressures of replacing oil-based materials and reducing the accumulation of litter in the environment, the use of bioplastics is increasing, despite little being known about their accurate biodegradation in natural conditions.					
36269217	8	28	theme	substantial	1408:1418	arg1	differences					1420:1430	substantial differences	1408:1430	substantial differences in the persistence and biodegradation rates among bioplastics	1408:1492	The results show substantial differences in the persistence and biodegradation rates among bioplastics, thus highlighting the need for carefully selecting materials for applications with risk of becoming marine litter.					
36269217	3	29	theme	weight	476:481	arg1	attrition					483:491	weight attrition	476:491	weight attrition	476:491	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	4	30	theme	Bacterial	739:747	arg1	communities					749:759	Bacterial communities	739:759	Bacterial communities	739:759	Bacterial communities were also investigated to verify whether putative plastic-degrading bacteria are enriched on bioplastics.					
36269217	3	31	theme	controlled	674:683	arg1	experiments					708:718	controlled 1 month biodegradation experiments	674:718	controlled 1 month biodegradation experiments in the laboratory	674:736	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	2	32	theme	oil-based	246:254	arg1	materials					256:264	oil-based materials	246:264	oil-based materials	246:264	As a result of the pressures of replacing oil-based materials and reducing the accumulation of litter in the environment, the use of bioplastics is increasing, despite little being known about their accurate biodegradation in natural conditions.					
36269217	3	33	from	incubation	629:638	arg1	Sea					663:665	the brackish Baltic Sea	643:665	the brackish Baltic Sea	643:665	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	5	34	theme	degradation	905:915	arg1	signs					896:900	no signs	893:900	no signs of degradation	893:915	Poly-l-lactic acid showed no signs of degradation, whereas poly(3-hydroxybutyrate/3-hydroxyvalerate) (PHB/HV), plasticized starch (PR), and cellulose acetate (CA) degraded completely or almost completely during 1-year in situ incubations.					
36269217	0	35	theme	Community	32:40	arg1	Compositions					42:53	Bacterial Community Compositions	22:53	Bacterial Community Compositions	22:53	Degradation Rates and Bacterial Community Compositions Vary among Commonly Used Bioplastic Materials in a Brackish Marine Environment.					
36269217	4	36	theme	putative	802:809	arg1	bacteria					829:836	putative plastic-degrading bacteria	802:836	putative plastic-degrading bacteria	802:836	Bacterial communities were also investigated to verify whether putative plastic-degrading bacteria are enriched on bioplastics.					
36269217	5	37	theme	plasticized	978:988	arg1	PR					998:999	PR	998:999	PR	998:999	Poly-l-lactic acid showed no signs of degradation, whereas poly(3-hydroxybutyrate/3-hydroxyvalerate) (PHB/HV), plasticized starch (PR), and cellulose acetate (CA) degraded completely or almost completely during 1-year in situ incubations.					
36269217	5	37	theme	plasticized	978:988	arg1	starch					990:995	plasticized starch	978:995	plasticized starch (PR)	978:1000	Poly-l-lactic acid showed no signs of degradation, whereas poly(3-hydroxybutyrate/3-hydroxyvalerate) (PHB/HV), plasticized starch (PR), and cellulose acetate (CA) degraded completely or almost completely during 1-year in situ incubations.					
36269217	2	38	theme	accurate	403:410	arg1	biodegradation					412:425	their accurate biodegradation	397:425	their accurate biodegradation in natural conditions	397:447	As a result of the pressures of replacing oil-based materials and reducing the accumulation of litter in the environment, the use of bioplastics is increasing, despite little being known about their accurate biodegradation in natural conditions.					
36269217	3	39	theme	1-year	614:619	arg1	incubation					629:638	a 1-year in situ incubation	612:638	a 1-year in situ incubation in the brackish Baltic Sea	612:665	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	8	40	with	applications	1560:1571	arg1	risk					1578:1581	risk	1578:1581	risk of becoming marine litter	1578:1607	The results show substantial differences in the persistence and biodegradation rates among bioplastics, thus highlighting the need for carefully selecting materials for applications with risk of becoming marine litter.					
36269217	3	41	theme	degradation	497:507	arg1	behavior					509:516	degradation behavior	497:516	degradation behavior	497:516	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	6	42	theme	class	1215:1219	arg1	Gammaproteobacteria					1221:1239	class Gammaproteobacteria	1215:1239	class Gammaproteobacteria	1215:1239	In accordance, bacterial taxa potentially capable of using complex carbon substrates and belonging, e.g., to class Gammaproteobacteria were significantly enriched on PHB/HV, PR, and CA.					
36269217	7	43	theme	biodegradation	1363:1376	arg1	experiments					1378:1388	the biodegradation experiments	1359:1388	the biodegradation experiments	1359:1388	An increase in gammaproteobacterial abundance was also observed in the biodegradation experiments.					
36269217	3	44	theme	biodegradation	693:706	arg1	experiments					708:718	controlled 1 month biodegradation experiments	674:718	controlled 1 month biodegradation experiments in the laboratory	674:736	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	2	45	theme	pressures	223:231	arg1	result					209:214	a result	207:214	a result of the pressures of replacing oil-based materials and reducing the accumulation of litter in the environment	207:323	As a result of the pressures of replacing oil-based materials and reducing the accumulation of litter in the environment, the use of bioplastics is increasing, despite little being known about their accurate biodegradation in natural conditions.					
36269217	2	46	theme	bioplastics	337:347	arg1	use					330:332	the use	326:332	the use of bioplastics	326:347	As a result of the pressures of replacing oil-based materials and reducing the accumulation of litter in the environment, the use of bioplastics is increasing, despite little being known about their accurate biodegradation in natural conditions.					
36269217	2	46	theme	bioplastics	337:347	arg1	increasing					352:361	increasing	352:361	increasing	352:361	As a result of the pressures of replacing oil-based materials and reducing the accumulation of litter in the environment, the use of bioplastics is increasing, despite little being known about their accurate biodegradation in natural conditions.					
36269217	8	47	theme	selecting	1536:1544	arg1	materials					1546:1554	carefully selecting materials	1526:1554	carefully selecting materials	1526:1554	The results show substantial differences in the persistence and biodegradation rates among bioplastics, thus highlighting the need for carefully selecting materials for applications with risk of becoming marine litter.					
36269217	3	48	theme	brackish	647:654	arg1	Sea					663:665	the brackish Baltic Sea	643:665	the brackish Baltic Sea	643:665	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	1	49	theme	Plastic	135:141	arg1	pollution					143:151	Plastic pollution	135:151	Plastic pollution	135:151	Plastic pollution threatens both terrestrial and aquatic ecosystems.					
36269217	2	50	from	accumulation	283:294	arg1	environment					313:323	the environment	309:323	the environment	309:323	As a result of the pressures of replacing oil-based materials and reducing the accumulation of litter in the environment, the use of bioplastics is increasing, despite little being known about their accurate biodegradation in natural conditions.					
36269217	3	51	theme	1	685:685	arg1	month					687:691	month	687:691	month	687:691	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	3	52	theme	Baltic	656:661	arg1	Sea					663:665	the brackish Baltic Sea	643:665	the brackish Baltic Sea	643:665	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	2	53	from	biodegradation	412:425	arg1	conditions					438:447	natural conditions	430:447	natural conditions	430:447	As a result of the pressures of replacing oil-based materials and reducing the accumulation of litter in the environment, the use of bioplastics is increasing, despite little being known about their accurate biodegradation in natural conditions.					
36269217	6	54	theme	capable	1148:1154	arg1	taxa					1131:1134	bacterial taxa	1121:1134	bacterial taxa potentially capable of using complex carbon substrates and belonging, e.g., to class Gammaproteobacteria	1121:1239	In accordance, bacterial taxa potentially capable of using complex carbon substrates and belonging, e.g., to class Gammaproteobacteria were significantly enriched on PHB/HV, PR, and CA.					
36269217	6	55	theme	complex	1165:1171	arg1	substrates					1180:1189	complex carbon substrates	1165:1189	complex carbon substrates	1165:1189	In accordance, bacterial taxa potentially capable of using complex carbon substrates and belonging, e.g., to class Gammaproteobacteria were significantly enriched on PHB/HV, PR, and CA.					
36269217	0	56	theme	Used	75:78	arg1	Materials					91:99	Commonly Used Bioplastic Materials	66:99	Commonly Used Bioplastic Materials	66:99	Degradation Rates and Bacterial Community Compositions Vary among Commonly Used Bioplastic Materials in a Brackish Marine Environment.					
36269217	3	57	theme	month	687:691	arg1	experiments					708:718	controlled 1 month biodegradation experiments	674:718	controlled 1 month biodegradation experiments in the laboratory	674:736	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	3	58	theme	different	526:534	arg1	materials					547:555	four different bioplastic materials	521:555	four different bioplastic materials	521:555	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
36269217	8	59	theme	persistence	1439:1449	arg1	rates					1470:1474	the persistence and biodegradation rates	1435:1474	the persistence and biodegradation rates among bioplastics	1435:1492	The results show substantial differences in the persistence and biodegradation rates among bioplastics, thus highlighting the need for carefully selecting materials for applications with risk of becoming marine litter.					
36269217	5	60	dep	in	1085:1086	arg1	situ					1088:1091	situ	1088:1091	situ	1088:1091	Poly-l-lactic acid showed no signs of degradation, whereas poly(3-hydroxybutyrate/3-hydroxyvalerate) (PHB/HV), plasticized starch (PR), and cellulose acetate (CA) degraded completely or almost completely during 1-year in situ incubations.					
36269217	3	61	theme	bioplastic	536:545	arg1	materials					547:555	four different bioplastic materials	521:555	four different bioplastic materials	521:555	Here, we investigated the weight attrition and degradation behavior of four different bioplastic materials compared to conventional oil-based polyethylene during a 1-year in situ incubation in the brackish Baltic Sea and in controlled 1 month biodegradation experiments in the laboratory.					
35987387	0	0	theme	cryopreservation	96:111	arg1	protocols					113:121	constant volume cryopreservation protocols	80:121	constant volume cryopreservation protocols	80:121	Temperature-pressure correlations of cryoprotective additives for the design of constant volume cryopreservation protocols.					
35987387	2	1	theme	constant	469:476	arg1	pressure					478:485	constant pressure	469:485	constant pressure	469:485	It has been established that the thermodynamics of isochoric freezing is different from that of isobaric (constant pressure) freezing.					
35987387	1	2	theme	constant	156:163	arg1	isochoric					173:181	isochoric	173:181	isochoric	173:181	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	1	2	theme	constant	156:163	arg1	volume					165:170	constant volume	156:170	constant volume (isochoric) cryopreservation	156:199	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	3	3	from	researchers	518:528	arg1	results					556:562	the field experimental results	533:562	the field experimental results	533:562	This study provides researchers in the field experimental results for various compositions of cryoprotectants commonly used in isobaric cryopreservation, in terms of temperature-pressure-molar concentration correlation.					
35987387	0	4	theme	volume	89:94	arg1	protocols					113:121	constant volume cryopreservation protocols	80:121	constant volume cryopreservation protocols	80:121	Temperature-pressure correlations of cryoprotective additives for the design of constant volume cryopreservation protocols.					
35987387	1	5	from	interest	319:326	arg1	use					335:337	the use	331:337	the use of this new technology	331:360	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	1	6	theme	volume	165:170	arg1	cryopreservation					184:199	constant volume (isochoric) cryopreservation	156:199	constant volume (isochoric) cryopreservation	156:199	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	4	7	dep	cryoprotectants	794:808	arg1	glycol					917:922	diethylene glycol	906:922	diethylene glycol	906:922	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	7	dep	cryoprotectants	794:808	arg1	cryoprotectants					794:808	the following cryoprotectants	780:808	the following cryoprotectants	780:808	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	7	dep	cryoprotectants	794:808	arg1	trehalose					875:883	trehalose	875:883	trehalose	875:883	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	7	dep	cryoprotectants	794:808	arg1	glycol					895:900	ethylene glycol	886:900	ethylene glycol	886:900	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	7	dep	cryoprotectants	794:808	arg1	sulfoxide					864:872	dimethyl sulfoxide	855:872	dimethyl sulfoxide	855:872	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	8	theme	isobaric	828:835	arg1	cryopreservation					837:852	isobaric cryopreservation	828:852	isobaric cryopreservation	828:852	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	2	9	dep	isobaric	459:466	arg1	pressure					478:485	constant pressure	469:485	constant pressure	469:485	It has been established that the thermodynamics of isochoric freezing is different from that of isobaric (constant pressure) freezing.					
35987387	4	10	theme	following	784:792	arg1	glycol					917:922	diethylene glycol	906:922	diethylene glycol	906:922	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	10	theme	following	784:792	arg1	cryoprotectants					794:808	the following cryoprotectants	780:808	the following cryoprotectants	780:808	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	10	theme	following	784:792	arg1	trehalose					875:883	trehalose	875:883	trehalose	875:883	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	10	theme	following	784:792	arg1	glycol					895:900	ethylene glycol	886:900	ethylene glycol	886:900	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	10	theme	following	784:792	arg1	sulfoxide					864:872	dimethyl sulfoxide	855:872	dimethyl sulfoxide	855:872	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	0	11	theme	protocols	113:121	arg1	design					70:75	the design	66:75	the design of constant volume cryopreservation protocols	66:121	Temperature-pressure correlations of cryoprotective additives for the design of constant volume cryopreservation protocols.					
35987387	2	12	from	that	451:454	arg1	different					436:444	different	436:444	different	436:444	It has been established that the thermodynamics of isochoric freezing is different from that of isobaric (constant pressure) freezing.					
35987387	2	12	from	that	451:454	arg1	thermodynamics					396:409	the thermodynamics	392:409	the thermodynamics of isochoric freezing	392:431	It has been established that the thermodynamics of isochoric freezing is different from that of isobaric (constant pressure) freezing.					
35987387	1	13	from	use	149:151	arg1	biotechnology					218:230	biotechnology	218:230	biotechnology	218:230	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	1	13	from	use	149:151	arg1	medicine					205:212	medicine	205:212	medicine	205:212	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	6	14	theme	isochoric	1244:1252	arg1	freezing					1254:1261	isochoric freezing	1244:1261	isochoric freezing	1244:1261	Our new experimental results indicate that the studied concentrations for each of the CPAs, lower and expands the range of temperatures in which cryopreservation by isochoric freezing can be safely practiced.					
35987387	1	15	theme	research	269:276	arg1	community					278:286	the research community	265:286	the research community	265:286	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	1	16	theme	new	347:349	arg1	technology					351:360	this new technology	342:360	this new technology	342:360	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	5	17	from	data	940:943	arg1	correlation					973:983	the pressure-temperature correlation	948:983	the pressure-temperature correlation in an isochoric system of cryoprotectants used in isobaric cryopreservation	948:1059	Currently, the data on the pressure-temperature correlation in an isochoric system of cryoprotectants used in isobaric cryopreservation is not available.					
35987387	5	18	theme	isobaric	1035:1042	arg1	cryopreservation					1044:1059	isobaric cryopreservation	1035:1059	isobaric cryopreservation	1035:1059	Currently, the data on the pressure-temperature correlation in an isochoric system of cryoprotectants used in isobaric cryopreservation is not available.					
35987387	6	19	from	cryopreservation	1224:1239	arg1	temperatures					1202:1213	temperatures	1202:1213	temperatures in which cryopreservation by isochoric freezing	1202:1261	Our new experimental results indicate that the studied concentrations for each of the CPAs, lower and expands the range of temperatures in which cryopreservation by isochoric freezing can be safely practiced.					
35987387	1	20	theme	technology	351:360	arg1	use					335:337	the use	331:337	the use of this new technology	331:360	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	4	21	theme	ethylene	886:893	arg1	cryoprotectants					794:808	the following cryoprotectants	780:808	the following cryoprotectants	780:808	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	21	theme	ethylene	886:893	arg1	glycol					895:900	ethylene glycol	886:900	ethylene glycol	886:900	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	22	theme	diethylene	906:915	arg1	cryoprotectants					794:808	the following cryoprotectants	780:808	the following cryoprotectants	780:808	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	22	theme	diethylene	906:915	arg1	glycol					917:922	diethylene glycol	906:922	diethylene glycol	906:922	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	5	23	theme	isochoric	991:999	arg1	system					1001:1006	an isochoric system	988:1006	an isochoric system of cryoprotectants used in isobaric cryopreservation	988:1059	Currently, the data on the pressure-temperature correlation in an isochoric system of cryoprotectants used in isobaric cryopreservation is not available.					
35987387	0	24	theme	Temperature-pressure	0:19	arg1	correlations					21:32	Temperature-pressure correlations	0:32	Temperature-pressure correlations of cryoprotective additives for the design of constant volume cryopreservation protocols	0:121	Temperature-pressure correlations of cryoprotective additives for the design of constant volume cryopreservation protocols.					
35987387	1	25	theme	cryopreservation	184:199	arg1	use					149:151	the use	145:151	the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology	145:230	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	0	26	theme	cryoprotective	37:50	arg1	additives					52:60	cryoprotective additives	37:60	cryoprotective additives	37:60	Temperature-pressure correlations of cryoprotective additives for the design of constant volume cryopreservation protocols.					
35987387	4	27	theme	isochoric	747:755	arg1	data					771:774	experimental isochoric thermodynamic data	734:774	experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol	734:922	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	3	28	theme	field	537:541	arg1	results					556:562	the field experimental results	533:562	the field experimental results	533:562	This study provides researchers in the field experimental results for various compositions of cryoprotectants commonly used in isobaric cryopreservation, in terms of temperature-pressure-molar concentration correlation.					
35987387	6	29	theme	studied	1126:1132	arg1	concentrations					1134:1147	the studied concentrations	1122:1147	the studied concentrations for each of the CPAs, lower and expands the range of temperatures in which cryopreservation by isochoric freezing	1122:1261	Our new experimental results indicate that the studied concentrations for each of the CPAs, lower and expands the range of temperatures in which cryopreservation by isochoric freezing can be safely practiced.					
35987387	2	30	theme	isochoric	414:422	arg1	freezing					424:431	isochoric freezing	414:431	isochoric freezing	414:431	It has been established that the thermodynamics of isochoric freezing is different from that of isobaric (constant pressure) freezing.					
35987387	1	31	dep	captured	236:243	arg1	interest					319:326	an increasing interest	305:326	an increasing interest in the use of this new technology	305:360	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	3	32	theme	experimental	543:554	arg1	results					556:562	the field experimental results	533:562	the field experimental results	533:562	This study provides researchers in the field experimental results for various compositions of cryoprotectants commonly used in isobaric cryopreservation, in terms of temperature-pressure-molar concentration correlation.					
35987387	2	33	theme	isobaric	459:466	arg1	freezing					488:495	isobaric (constant pressure) freezing	459:495	isobaric (constant pressure) freezing	459:495	It has been established that the thermodynamics of isochoric freezing is different from that of isobaric (constant pressure) freezing.					
35987387	7	34	theme	isochoric	1359:1367	arg1	protocols					1386:1394	new isochoric cryopreservation protocols	1355:1394	new isochoric cryopreservation protocols	1355:1394	We consider that these experiments will aid researchers developing new isochoric cryopreservation protocols.					
35987387	6	35	dep	CPAs	1165:1168	arg1	range					1193:1197	the range	1189:1197	the range of temperatures in which cryopreservation by isochoric freezing	1189:1261	Our new experimental results indicate that the studied concentrations for each of the CPAs, lower and expands the range of temperatures in which cryopreservation by isochoric freezing can be safely practiced.					
35987387	7	36	theme	cryopreservation	1369:1384	arg1	protocols					1386:1394	new isochoric cryopreservation protocols	1355:1394	new isochoric cryopreservation protocols	1355:1394	We consider that these experiments will aid researchers developing new isochoric cryopreservation protocols.					
35987387	0	37	theme	additives	52:60	arg1	correlations					21:32	Temperature-pressure correlations	0:32	Temperature-pressure correlations of cryoprotective additives for the design of constant volume cryopreservation protocols	0:121	Temperature-pressure correlations of cryoprotective additives for the design of constant volume cryopreservation protocols.					
35987387	2	38	theme	freezing	424:431	arg1	different					436:444	different	436:444	different	436:444	It has been established that the thermodynamics of isochoric freezing is different from that of isobaric (constant pressure) freezing.					
35987387	2	38	theme	freezing	424:431	arg1	thermodynamics					396:409	the thermodynamics	392:409	the thermodynamics of isochoric freezing	392:431	It has been established that the thermodynamics of isochoric freezing is different from that of isobaric (constant pressure) freezing.					
35987387	1	39	from	medicine	205:212	arg1	use					149:151	the use	145:151	the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology	145:230	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	3	40	theme	various	568:574	arg1	compositions					576:587	various compositions	568:587	various compositions of cryoprotectants commonly used in isobaric cryopreservation	568:649	This study provides researchers in the field experimental results for various compositions of cryoprotectants commonly used in isobaric cryopreservation, in terms of temperature-pressure-molar concentration correlation.					
35987387	1	41	theme	recent	131:136	arg1	years					138:142	the recent years	127:142	the recent years	127:142	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	1	42	theme	increasing	308:317	arg1	interest					319:326	an increasing interest	305:326	an increasing interest in the use of this new technology	305:360	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	3	43	theme	concentration	691:703	arg1	correlation					705:715	temperature-pressure-molar concentration correlation	664:715	temperature-pressure-molar concentration correlation	664:715	This study provides researchers in the field experimental results for various compositions of cryoprotectants commonly used in isobaric cryopreservation, in terms of temperature-pressure-molar concentration correlation.					
35987387	7	44	theme	new	1355:1357	arg1	protocols					1386:1394	new isochoric cryopreservation protocols	1355:1394	new isochoric cryopreservation protocols	1355:1394	We consider that these experiments will aid researchers developing new isochoric cryopreservation protocols.					
35987387	4	45	theme	experimental	734:745	arg1	data					771:774	experimental isochoric thermodynamic data	734:774	experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol	734:922	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	5	46	from	correlation	973:983	arg1	system					1001:1006	an isochoric system	988:1006	an isochoric system of cryoprotectants used in isobaric cryopreservation	988:1059	Currently, the data on the pressure-temperature correlation in an isochoric system of cryoprotectants used in isobaric cryopreservation is not available.					
35987387	1	47	from	biotechnology	218:230	arg1	use					149:151	the use	145:151	the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology	145:230	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	3	48	theme	correlation	705:715	arg1	terms					655:659	terms	655:659	terms of temperature-pressure-molar concentration correlation	655:715	This study provides researchers in the field experimental results for various compositions of cryoprotectants commonly used in isobaric cryopreservation, in terms of temperature-pressure-molar concentration correlation.					
35987387	3	49	theme	cryoprotectants	592:606	arg1	compositions					576:587	various compositions	568:587	various compositions of cryoprotectants commonly used in isobaric cryopreservation	568:649	This study provides researchers in the field experimental results for various compositions of cryoprotectants commonly used in isobaric cryopreservation, in terms of temperature-pressure-molar concentration correlation.					
35987387	6	50	theme	temperatures	1202:1213	arg1	range					1193:1197	the range	1189:1197	the range of temperatures in which cryopreservation by isochoric freezing	1189:1261	Our new experimental results indicate that the studied concentrations for each of the CPAs, lower and expands the range of temperatures in which cryopreservation by isochoric freezing can be safely practiced.					
35987387	5	51	theme	pressure-temperature	952:971	arg1	correlation					973:983	the pressure-temperature correlation	948:983	the pressure-temperature correlation in an isochoric system of cryoprotectants used in isobaric cryopreservation	948:1059	Currently, the data on the pressure-temperature correlation in an isochoric system of cryoprotectants used in isobaric cryopreservation is not available.					
35987387	3	52	theme	isobaric	625:632	arg1	cryopreservation					634:649	isobaric cryopreservation	625:649	isobaric cryopreservation	625:649	This study provides researchers in the field experimental results for various compositions of cryoprotectants commonly used in isobaric cryopreservation, in terms of temperature-pressure-molar concentration correlation.					
35987387	4	53	theme	thermodynamic	757:769	arg1	data					771:774	experimental isochoric thermodynamic data	734:774	experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol	734:922	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	6	54	theme	experimental	1087:1098	arg1	results					1100:1106	Our new experimental results	1079:1106	Our new experimental results	1079:1106	Our new experimental results indicate that the studied concentrations for each of the CPAs, lower and expands the range of temperatures in which cryopreservation by isochoric freezing can be safely practiced.					
35987387	0	55	theme	constant	80:87	arg1	protocols					113:121	constant volume cryopreservation protocols	80:121	constant volume cryopreservation protocols	80:121	Temperature-pressure correlations of cryoprotective additives for the design of constant volume cryopreservation protocols.					
35987387	3	56	theme	temperature-pressure-molar	664:689	arg1	correlation					705:715	temperature-pressure-molar concentration correlation	664:715	temperature-pressure-molar concentration correlation	664:715	This study provides researchers in the field experimental results for various compositions of cryoprotectants commonly used in isobaric cryopreservation, in terms of temperature-pressure-molar concentration correlation.					
35987387	4	57	theme	dimethyl	855:862	arg1	cryoprotectants					794:808	the following cryoprotectants	780:808	the following cryoprotectants	780:808	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	4	57	theme	dimethyl	855:862	arg1	sulfoxide					864:872	dimethyl sulfoxide	855:872	dimethyl sulfoxide	855:872	It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol.					
35987387	1	58	from	cryopreservation	184:199	arg1	biotechnology					218:230	biotechnology	218:230	biotechnology	218:230	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	1	58	from	cryopreservation	184:199	arg1	medicine					205:212	medicine	205:212	medicine	205:212	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
35987387	5	59	theme	cryoprotectants	1011:1025	arg1	system					1001:1006	an isochoric system	988:1006	an isochoric system of cryoprotectants used in isobaric cryopreservation	988:1059	Currently, the data on the pressure-temperature correlation in an isochoric system of cryoprotectants used in isobaric cryopreservation is not available.					
35987387	6	60	theme	new	1083:1085	arg1	results					1100:1106	Our new experimental results	1079:1106	Our new experimental results	1079:1106	Our new experimental results indicate that the studied concentrations for each of the CPAs, lower and expands the range of temperatures in which cryopreservation by isochoric freezing can be safely practiced.					
35987387	1	61	theme	more	245:248	arg1	attention					250:258	more attention	245:258	more attention	245:258	In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology.					
37230613	8	0	theme	high	1075:1078	arg1	degree					1080:1085	a high degree	1073:1085	a high degree of crosslinking	1073:1101	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	6	1	theme	absorption	839:848	arg1	capacity					850:857	a considerable oil absorption capacity	820:857	a considerable oil absorption capacity of up to 29.26 g/g	820:876	Cryogel templates could reach a considerable oil absorption capacity of up to 29.26 g/g and prevent oil from leaking effectively.					
37230613	1	2	theme	chitosan-gelatin	174:189	arg1	conjugates					191:200	chitosan-gelatin conjugates	174:200	chitosan-gelatin conjugates	174:200	In this study, chitosan-gelatin conjugates were prepared by chemical crosslinking of tannic acid.					
37230613	4	3	link	crosslinked	632:642	arg1	samples					644:650	crosslinked samples	632:650	crosslinked samples	632:650	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	4	4	theme	bonding	679:685	arg1	strength					687:694	higher hydrogen bonding strength	663:694	higher hydrogen bonding strength	663:694	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	0	5	theme	oxidation	94:102	arg1	resistance					104:113	oxidation resistance	94:113	oxidation resistance	94:113	Characterization of chitosan-gelatin cryogel templates developed by chemical crosslinking and oxidation resistance of camellia oil cryogel-templated oleogels.					
37230613	5	6	theme	Tannic	697:702	arg1	crosslinking					709:720	Tannic acid crosslinking	697:720	Tannic acid crosslinking	697:720	Tannic acid crosslinking also led to enhanced thermal stabilities and mechanical properties.					
37230613	4	7	with	microstructures	578:592	arg1	porosities					604:613	high porosities	599:613	high porosities (over 96 %)	599:625	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	4	7	with	microstructures	578:592	arg1	%					624:624	over 96 %	616:624	over 96 %	616:624	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	4	8	theme	hydrogen	670:677	arg1	strength					687:694	higher hydrogen bonding strength	663:694	higher hydrogen bonding strength	663:694	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	0	9	theme	camellia	118:125	arg1	oleogels					149:156	camellia oil cryogel-templated oleogels	118:156	camellia oil cryogel-templated oleogels	118:156	Characterization of chitosan-gelatin cryogel templates developed by chemical crosslinking and oxidation resistance of camellia oil cryogel-templated oleogels.					
37230613	5	10	theme	mechanical	767:776	arg1	properties					778:787	mechanical properties	767:787	mechanical properties	767:787	Tannic acid crosslinking also led to enhanced thermal stabilities and mechanical properties.					
37230613	5	11	theme	acid	704:707	arg1	crosslinking					709:720	Tannic acid crosslinking	697:720	Tannic acid crosslinking	697:720	Tannic acid crosslinking also led to enhanced thermal stabilities and mechanical properties.					
37230613	2	12	theme	camellia	332:339	arg1	oil					341:343	camellia oil	332:343	camellia oil to construct cryogel-templated oleogels	332:383	The cryogel templates were developed through freeze-drying and immersed in camellia oil to construct cryogel-templated oleogels.					
37230613	4	13	contain	have	658:661	arg1	samples					644:650	crosslinked samples	632:650	crosslinked samples	632:650	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	4	13	contain	have	658:661	arg2	strength					687:694	higher hydrogen bonding strength	663:694	higher hydrogen bonding strength	663:694	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	3	14	theme	colour	429:434	arg1	changes					436:442	apparent colour changes	420:442	apparent colour changes	420:442	Chemical crosslinking resulted in apparent colour changes and improved emulsion-related/rheological properties on conjugates.					
37230613	3	15	from	changes	436:442	arg1	conjugates					500:509	conjugates	500:509	conjugates	500:509	Chemical crosslinking resulted in apparent colour changes and improved emulsion-related/rheological properties on conjugates.					
37230613	8	16	theme	lowest	1113:1118	arg1	39.74 nmol/kg					1142:1154	39.74 nmol/kg	1142:1154	39.74 nmol/kg	1142:1154	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	8	16	theme	lowest	1113:1118	arg1	24.40 μg/g					1161:1170	24.40 μg/g	1161:1170	24.40 μg/g	1161:1170	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	8	16	theme	lowest	1113:1118	arg1	values					1134:1139	POV and TBARS values	1120:1139	the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively)	1109:1185	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	9	17	from	acid	1359:1362	arg1	systems					1392:1398	the composite biopolymer systems	1367:1398	the composite biopolymer systems	1367:1398	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	6	18	theme	oil	835:837	arg1	capacity					850:857	a considerable oil absorption capacity	820:857	a considerable oil absorption capacity of up to 29.26 g/g	820:876	Cryogel templates could reach a considerable oil absorption capacity of up to 29.26 g/g and prevent oil from leaking effectively.					
37230613	0	19	theme	cryogel-templated	131:147	arg1	oleogels					149:156	camellia oil cryogel-templated oleogels	118:156	camellia oil cryogel-templated oleogels	118:156	Characterization of chitosan-gelatin cryogel templates developed by chemical crosslinking and oxidation resistance of camellia oil cryogel-templated oleogels.					
37230613	7	20	theme	outstanding	982:992	arg1	abilities					1006:1014	outstanding antioxidant abilities	982:1014	outstanding antioxidant abilities	982:1014	The obtained oleogels with high tannic acid content possessed outstanding antioxidant abilities.					
37230613	9	21	theme	cryogel-templated	1316:1332	arg1	oleogels					1334:1341	cryogel-templated oleogels	1316:1341	cryogel-templated oleogels	1316:1341	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	6	22	theme	considerable	822:833	arg1	capacity					850:857	a considerable oil absorption capacity	820:857	a considerable oil absorption capacity of up to 29.26 g/g	820:876	Cryogel templates could reach a considerable oil absorption capacity of up to 29.26 g/g and prevent oil from leaking effectively.					
37230613	0	23	theme	oil	127:129	arg1	oleogels					149:156	camellia oil cryogel-templated oleogels	118:156	camellia oil cryogel-templated oleogels	118:156	Characterization of chitosan-gelatin cryogel templates developed by chemical crosslinking and oxidation resistance of camellia oil cryogel-templated oleogels.					
37230613	9	24	theme	oleogels	1334:1341	arg1	preparation					1271:1281	the preparation	1267:1281	the preparation	1267:1281	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	9	24	theme	oleogels	1334:1341	arg1	application					1291:1301	the application potential	1287:1311	the application potential of cryogel-templated oleogels	1287:1341	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	8	25	with	Oleogels	1059:1066	arg1	degree					1080:1085	a high degree	1073:1085	a high degree of crosslinking	1073:1101	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	3	26	theme	improved	448:455	arg1	properties					486:495	improved emulsion-related/rheological properties	448:495	improved emulsion-related/rheological properties	448:495	Chemical crosslinking resulted in apparent colour changes and improved emulsion-related/rheological properties on conjugates.					
37230613	4	27	theme	high	599:602	arg1	porosities					604:613	high porosities	599:613	high porosities (over 96 %)	599:625	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	4	27	theme	high	599:602	arg1	%					624:624	over 96 %	616:624	over 96 %	616:624	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	5	28	theme	enhanced	734:741	arg1	stabilities					751:761	enhanced thermal stabilities	734:761	enhanced thermal stabilities	734:761	Tannic acid crosslinking also led to enhanced thermal stabilities and mechanical properties.					
37230613	8	29	theme	POV	1120:1122	arg1	39.74 nmol/kg					1142:1154	39.74 nmol/kg	1142:1154	39.74 nmol/kg	1142:1154	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	8	29	theme	POV	1120:1122	arg1	24.40 μg/g					1161:1170	24.40 μg/g	1161:1170	24.40 μg/g	1161:1170	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	8	29	theme	POV	1120:1122	arg1	values					1134:1139	POV and TBARS values	1120:1139	the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively)	1109:1185	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	2	30	theme	cryogel-templated	358:374	arg1	oleogels					376:383	cryogel-templated oleogels	358:383	cryogel-templated oleogels	358:383	The cryogel templates were developed through freeze-drying and immersed in camellia oil to construct cryogel-templated oleogels.					
37230613	3	31	theme	emulsion-related/rheological	457:484	arg1	properties					486:495	improved emulsion-related/rheological properties	448:495	improved emulsion-related/rheological properties	448:495	Chemical crosslinking resulted in apparent colour changes and improved emulsion-related/rheological properties on conjugates.					
37230613	7	32	theme	obtained	924:931	arg1	oleogels					933:940	The obtained oleogels	920:940	The obtained oleogels with high tannic acid content	920:970	The obtained oleogels with high tannic acid content possessed outstanding antioxidant abilities.					
37230613	5	33	theme	thermal	743:749	arg1	stabilities					751:761	enhanced thermal stabilities	734:761	enhanced thermal stabilities	734:761	Tannic acid crosslinking also led to enhanced thermal stabilities and mechanical properties.					
37230613	9	34	theme	composite	1371:1379	arg1	systems					1392:1398	the composite biopolymer systems	1367:1398	the composite biopolymer systems	1367:1398	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	7	35	theme	tannic	952:957	arg1	content					964:970	high tannic acid content	947:970	high tannic acid content	947:970	The obtained oleogels with high tannic acid content possessed outstanding antioxidant abilities.					
37230613	1	36	theme	chemical	219:226	arg1	crosslinking					228:239	chemical crosslinking	219:239	chemical crosslinking of tannic acid	219:254	In this study, chitosan-gelatin conjugates were prepared by chemical crosslinking of tannic acid.					
37230613	0	37	theme	cryogel	37:43	arg1	templates					45:53	chitosan-gelatin cryogel templates	20:53	chitosan-gelatin cryogel templates	20:53	Characterization of chitosan-gelatin cryogel templates developed by chemical crosslinking and oxidation resistance of camellia oil cryogel-templated oleogels.					
37230613	7	38	with	oleogels	933:940	arg1	content					964:970	high tannic acid content	947:970	high tannic acid content	947:970	The obtained oleogels with high tannic acid content possessed outstanding antioxidant abilities.					
37230613	9	39	theme	potential	1303:1311	arg1	application					1291:1301	the application potential	1287:1311	the application potential of cryogel-templated oleogels	1287:1341	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	7	40	theme	acid	959:962	arg1	content					964:970	high tannic acid content	947:970	high tannic acid content	947:970	The obtained oleogels with high tannic acid content possessed outstanding antioxidant abilities.					
37230613	0	41	theme	chitosan-gelatin	20:35	arg1	templates					45:53	chitosan-gelatin cryogel templates	20:53	chitosan-gelatin cryogel templates	20:53	Characterization of chitosan-gelatin cryogel templates developed by chemical crosslinking and oxidation resistance of camellia oil cryogel-templated oleogels.					
37230613	4	42	theme	higher	663:668	arg1	strength					687:694	higher hydrogen bonding strength	663:694	higher hydrogen bonding strength	663:694	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	0	43	theme	oleogels	149:156	arg1	crosslinking					77:88	chemical crosslinking	68:88	chemical crosslinking	68:88	Characterization of chitosan-gelatin cryogel templates developed by chemical crosslinking and oxidation resistance of camellia oil cryogel-templated oleogels.					
37230613	0	43	theme	oleogels	149:156	arg1	resistance					104:113	oxidation resistance	94:113	oxidation resistance	94:113	Characterization of chitosan-gelatin cryogel templates developed by chemical crosslinking and oxidation resistance of camellia oil cryogel-templated oleogels.					
37230613	9	44	theme	tannic	1352:1357	arg1	agent					1435:1439	the crosslinking agent	1418:1439	the crosslinking agent	1418:1439	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	9	44	theme	tannic	1352:1357	arg1	antioxidant					1449:1459	antioxidant	1449:1459	antioxidant	1449:1459	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	9	44	theme	tannic	1352:1357	arg1	acid					1359:1362	the tannic acid	1348:1362	the tannic acid in the composite biopolymer systems	1348:1398	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	8	45	theme	oxidation	1039:1047	arg1	8 days					1023:1028	8 days	1023:1028	8 days of rapid oxidation at 40 °C	1023:1056	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	6	46	theme	Cryogel	790:796	arg1	templates					798:806	Cryogel templates	790:806	Cryogel templates	790:806	Cryogel templates could reach a considerable oil absorption capacity of up to 29.26 g/g and prevent oil from leaking effectively.					
37230613	4	47	with	templates	524:532	arg1	formulas					549:556	different formulas	539:556	different formulas	539:556	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	7	48	theme	antioxidant	994:1004	arg1	abilities					1006:1014	outstanding antioxidant abilities	982:1014	outstanding antioxidant abilities	982:1014	The obtained oleogels with high tannic acid content possessed outstanding antioxidant abilities.					
37230613	4	49	theme	different	539:547	arg1	formulas					549:556	different formulas	539:556	different formulas	539:556	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	8	50	from	8 days	1023:1028	arg1	40 °C					1052:1056	40 °C	1052:1056	40 °C	1052:1056	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	7	51	theme	high	947:950	arg1	content					964:970	high tannic acid content	947:970	high tannic acid content	947:970	The obtained oleogels with high tannic acid content possessed outstanding antioxidant abilities.					
37230613	1	52	theme	tannic	244:249	arg1	acid					251:254	tannic acid	244:254	tannic acid	244:254	In this study, chitosan-gelatin conjugates were prepared by chemical crosslinking of tannic acid.					
37230613	0	53	theme	templates	45:53	arg1	Characterization					0:15	Characterization	0:15	Characterization of chitosan-gelatin cryogel templates	0:53	Characterization of chitosan-gelatin cryogel templates developed by chemical crosslinking and oxidation resistance of camellia oil cryogel-templated oleogels.					
37230613	4	54	theme	different	568:576	arg1	microstructures					578:592	different microstructures	568:592	different microstructures with high porosities (over 96 %)	568:625	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	2	55	theme	cryogel	261:267	arg1	templates					269:277	The cryogel templates	257:277	The cryogel templates	257:277	The cryogel templates were developed through freeze-drying and immersed in camellia oil to construct cryogel-templated oleogels.					
37230613	9	56	theme	chemical	1233:1240	arg1	crosslinking					1242:1253	chemical crosslinking	1233:1253	chemical crosslinking	1233:1253	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	1	57	theme	acid	251:254	arg1	crosslinking					228:239	chemical crosslinking	219:239	chemical crosslinking of tannic acid	219:254	In this study, chitosan-gelatin conjugates were prepared by chemical crosslinking of tannic acid.					
37230613	0	58	theme	chemical	68:75	arg1	crosslinking					77:88	chemical crosslinking	68:88	chemical crosslinking	68:88	Characterization of chitosan-gelatin cryogel templates developed by chemical crosslinking and oxidation resistance of camellia oil cryogel-templated oleogels.					
37230613	3	59	theme	apparent	420:427	arg1	changes					436:442	apparent colour changes	420:442	apparent colour changes	420:442	Chemical crosslinking resulted in apparent colour changes and improved emulsion-related/rheological properties on conjugates.					
37230613	7	60	contain	possessed	972:980	arg2	abilities					1006:1014	outstanding antioxidant abilities	982:1014	outstanding antioxidant abilities	982:1014	The obtained oleogels with high tannic acid content possessed outstanding antioxidant abilities.					
37230613	7	60	contain	possessed	972:980	arg1	oleogels					933:940	The obtained oleogels	920:940	The obtained oleogels with high tannic acid content	920:970	The obtained oleogels with high tannic acid content possessed outstanding antioxidant abilities.					
37230613	9	61	theme	crosslinking	1242:1253	arg1	involvement					1218:1228	the involvement	1214:1228	the involvement of chemical crosslinking	1214:1253	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	4	62	theme	crosslinked	632:642	arg1	samples					644:650	crosslinked samples	632:650	crosslinked samples	632:650	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	4	63	theme	cryogel	516:522	arg1	templates					524:532	The cryogel templates	512:532	The cryogel templates with different formulas	512:556	The cryogel templates with different formulas exhibited different microstructures with high porosities (over 96 %), and crosslinked samples might have higher hydrogen bonding strength.					
37230613	8	64	theme	rapid	1033:1037	arg1	oxidation					1039:1047	rapid oxidation	1033:1047	rapid oxidation at 40 °C	1033:1056	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	8	65	dep	values	1134:1139	arg1	39.74 nmol/kg					1142:1154	39.74 nmol/kg	1142:1154	39.74 nmol/kg	1142:1154	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	8	65	dep	values	1134:1139	arg1	24.40 μg/g					1161:1170	24.40 μg/g	1161:1170	24.40 μg/g	1161:1170	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	8	65	dep	values	1134:1139	arg1	values					1134:1139	POV and TBARS values	1120:1139	the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively)	1109:1185	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	8	66	theme	TBARS	1128:1132	arg1	39.74 nmol/kg					1142:1154	39.74 nmol/kg	1142:1154	39.74 nmol/kg	1142:1154	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	8	66	theme	TBARS	1128:1132	arg1	24.40 μg/g					1161:1170	24.40 μg/g	1161:1170	24.40 μg/g	1161:1170	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	8	66	theme	TBARS	1128:1132	arg1	values					1134:1139	POV and TBARS values	1120:1139	the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively)	1109:1185	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	9	67	theme	biopolymer	1381:1390	arg1	systems					1392:1398	the composite biopolymer systems	1367:1398	the composite biopolymer systems	1367:1398	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	8	68	theme	crosslinking	1090:1101	arg1	degree					1080:1085	a high degree	1073:1085	a high degree of crosslinking	1073:1101	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	8	69	from	40 °C	1052:1056	arg1	8 days					1023:1028	8 days	1023:1028	8 days of rapid oxidation at 40 °C	1023:1056	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	8	69	from	40 °C	1052:1056	arg1	oxidation					1039:1047	rapid oxidation	1033:1047	rapid oxidation at 40 °C	1033:1056	After 8 days of rapid oxidation at 40 °C, Oleogels with a high degree of crosslinking owned the lowest POV and TBARS values (39.74 nmol/kg, and 24.40 μg/g, respectively).					
37230613	6	70	theme	up	862:863	arg1	capacity					850:857	a considerable oil absorption capacity	820:857	a considerable oil absorption capacity of up to 29.26 g/g	820:876	Cryogel templates could reach a considerable oil absorption capacity of up to 29.26 g/g and prevent oil from leaking effectively.					
37230613	3	71	theme	Chemical	386:393	arg1	crosslinking					395:406	Chemical crosslinking	386:406	Chemical crosslinking	386:406	Chemical crosslinking resulted in apparent colour changes and improved emulsion-related/rheological properties on conjugates.					
37230613	6	72	from	leaking	899:905	arg1	oil					890:892	oil	890:892	oil from leaking	890:905	Cryogel templates could reach a considerable oil absorption capacity of up to 29.26 g/g and prevent oil from leaking effectively.					
37230613	3	73	from	properties	486:495	arg1	conjugates					500:509	conjugates	500:509	conjugates	500:509	Chemical crosslinking resulted in apparent colour changes and improved emulsion-related/rheological properties on conjugates.					
37230613	9	74	theme	crosslinking	1422:1433	arg1	agent					1435:1439	the crosslinking agent	1418:1439	the crosslinking agent	1418:1439	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37230613	9	74	theme	crosslinking	1422:1433	arg1	acid					1359:1362	the tannic acid	1348:1362	the tannic acid in the composite biopolymer systems	1348:1398	This study indicates that the involvement of chemical crosslinking would favor the preparation and the application potential of cryogel-templated oleogels, and the tannic acid in the composite biopolymer systems could act as both the crosslinking agent and the antioxidant.					
37305414	8	0	theme	Relative	1116:1123	arg1	abundance					1125:1133	Relative abundance	1116:1133	Relative abundance of several membrane-associated virulence factors that are crucial for adherence to the airway mucosa	1116:1234	Relative abundance of several membrane-associated virulence factors that are crucial for adherence to the airway mucosa, and serum resistance were altered.					
37305414	1	1	theme	diseases	316:323	arg1	range					300:304	a wide range	293:304	a wide range of airway diseases	293:323	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	1	2	theme	human	266:270	arg1	influenzae					229:238	Non-typeable Haemophilus influenzae	204:238	Non-typeable Haemophilus influenzae (NTHi)	204:245	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	1	2	theme	human	266:270	arg1	pathogen					272:279	a Gram-negative human pathogen	250:279	a Gram-negative human pathogen that causes a wide range of airway diseases	250:323	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	8	3	theme	membrane-associated	1146:1164	arg1	factors					1176:1182	several membrane-associated virulence factors	1138:1182	several membrane-associated virulence factors that are crucial for adherence to the airway mucosa	1138:1234	Relative abundance of several membrane-associated virulence factors that are crucial for adherence to the airway mucosa, and serum resistance were altered.					
37305414	4	4	theme	outer	648:652	arg1	OM					664:665	OM	664:665	OM	664:665	Here, we report a novel role of P5 in maintaining bacterial outer membrane (OM) integrity and protein composition important for NTHi-host interactions.					
37305414	4	4	theme	outer	648:652	arg1	membrane					654:661	bacterial outer membrane	638:661	bacterial outer membrane (OM) integrity	638:676	Here, we report a novel role of P5 in maintaining bacterial outer membrane (OM) integrity and protein composition important for NTHi-host interactions.					
37305414	7	5	theme	NTHi	1071:1074	arg1	strains					1063:1069	the strains NTHi 3655Δp5 and NTHi 3655Δp5	1059:1099	the strains NTHi 3655Δp5 and NTHi 3655Δp5	1059:1099	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	7	5	theme	NTHi	1071:1074	arg1	3655Δp5					1076:1082	NTHi 3655Δp5	1071:1082	NTHi 3655Δp5	1071:1082	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	5	6	theme	C-terminal	817:826	arg1	CTD					836:838	CTD	836:838	CTD	836:838	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	5	6	theme	C-terminal	817:826	arg1	P5					844:845	P5	844:845	P5	844:845	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	5	6	theme	C-terminal	817:826	arg1	domain					828:833	the periplasmic C-terminal domain	801:833	the periplasmic C-terminal domain (CTD) of P5	801:845	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	8	7	theme	factors	1176:1182	arg1	resistance					1247:1256	serum resistance	1241:1256	serum resistance	1241:1256	Relative abundance of several membrane-associated virulence factors that are crucial for adherence to the airway mucosa, and serum resistance were altered.					
37305414	8	7	theme	factors	1176:1182	arg1	abundance					1125:1133	Relative abundance	1116:1133	Relative abundance of several membrane-associated virulence factors that are crucial for adherence to the airway mucosa	1116:1234	Relative abundance of several membrane-associated virulence factors that are crucial for adherence to the airway mucosa, and serum resistance were altered.					
37305414	10	8	theme	β-lactam	1550:1557	arg1	antibiotics					1559:1569	the β-lactam antibiotics	1546:1569	the β-lactam antibiotics in both mutants	1546:1585	We found (i) a decreased adherence to airway epithelial cells and fibronectin, (ii) increased complement-mediated killing, and (iii) increased sensitivity to the β-lactam antibiotics in both mutants compared to NTHi 3655 wild-type.					
37305414	3	9	theme	regulators	576:585	arg1	recruitment					550:560	the recruitment	546:560	the recruitment of complement regulators	546:585	We previously showed that the outer membrane protein P5 contributes to bacterial serum resistance by the recruitment of complement regulators.					
37305414	10	10	dep	increased	1521:1529	arg1	iii					1516:1518	iii	1516:1518	iii	1516:1518	We found (i) a decreased adherence to airway epithelial cells and fibronectin, (ii) increased complement-mediated killing, and (iii) increased sensitivity to the β-lactam antibiotics in both mutants compared to NTHi 3655 wild-type.					
37305414	6	11	with	complex	913:919	arg1	peptidoglycan					926:938	peptidoglycan	926:938	peptidoglycan	926:938	In a peptidoglycan-binding assay, the CTD of P5 (P5CTD) formed a complex with peptidoglycan.					
37305414	8	12	theme	serum	1241:1245	arg1	resistance					1247:1256	serum resistance	1241:1256	serum resistance	1241:1256	Relative abundance of several membrane-associated virulence factors that are crucial for adherence to the airway mucosa, and serum resistance were altered.					
37305414	1	13	theme	Haemophilus	217:227	arg1	pathogen					272:279	a Gram-negative human pathogen	250:279	a Gram-negative human pathogen that causes a wide range of airway diseases	250:323	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	1	13	theme	Haemophilus	217:227	arg1	NTHi					241:244	NTHi	241:244	NTHi	241:244	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	1	13	theme	Haemophilus	217:227	arg1	influenzae					229:238	Non-typeable Haemophilus influenzae	204:238	Non-typeable Haemophilus influenzae (NTHi)	204:245	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	7	14	theme	membrane	1027:1034	arg1	composition					1044:1054	the membrane protein composition	1023:1054	the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5	1023:1099	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	7	15	theme	CTD	994:996	arg1	deletion					982:989	deletion	982:989	deletion of CTD or the entire P5	982:1013	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	5	16	theme	P5	844:845	arg1	CTD					836:838	CTD	836:838	CTD	836:838	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	5	16	theme	P5	844:845	arg1	P5					844:845	P5	844:845	P5	844:845	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	5	16	theme	P5	844:845	arg1	domain					828:833	the periplasmic C-terminal domain	801:833	the periplasmic C-terminal domain (CTD) of P5	801:845	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	0	17	theme	crucial	158:164	arg1	composition					146:156	the membrane protein composition	125:156	the membrane protein composition crucial for interactions with the human host	125:201	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	7	18	theme	entire	1005:1010	arg1	P5					1012:1013	the entire P5	1001:1013	the entire P5	1001:1013	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	10	19	from	antibiotics	1559:1569	arg1	mutants					1579:1585	both mutants	1574:1585	both mutants	1574:1585	We found (i) a decreased adherence to airway epithelial cells and fibronectin, (ii) increased complement-mediated killing, and (iii) increased sensitivity to the β-lactam antibiotics in both mutants compared to NTHi 3655 wild-type.					
37305414	7	20	theme	profiling	949:957	arg1	analysis					959:966	Protein profiling analysis	941:966	Protein profiling analysis	941:966	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	0	21	with	interactions	170:181	arg1	host					198:201	the human host	188:201	the human host	188:201	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	11	22	theme	wild-type	1739:1747	arg1	bacteria					1749:1756	the parent wild-type bacteria	1728:1756	the parent wild-type bacteria	1728:1756	These mutants were also more sensitive to lysis at hyperosmotic conditions and hypervesiculated compared to the parent wild-type bacteria.					
37305414	0	23	theme	bacterial	83:91	arg1	stability					102:110	bacterial membrane stability	83:110	bacterial membrane stability	83:110	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	12	24	theme	membrane	1872:1879	arg1	proteome					1881:1888	the membrane proteome	1868:1888	the membrane proteome	1868:1888	In conclusion, our results suggest that P5 is important for bacterial OM stability, which ultimately affects the membrane proteome and NTHi pathogenesis.					
37305414	5	25	from	domain	828:833	arg1	motif					792:796	a peptidoglycan-binding motif	768:796	a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5	768:845	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	2	26	theme	host	390:393	arg1	system					402:407	the host immune system	386:407	the host immune system	386:407	NTHi has a plethora of mechanisms to colonize while evading the host immune system for the establishment of infection.					
37305414	5	27	theme	In	740:741	arg1	analysis					750:757	In silico analysis	740:757	In silico analysis	740:757	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	0	28	theme	membrane	48:55	arg1	P5					65:66	Non-typeable Haemophilus influenzae major outer membrane protein P5	0:66	Non-typeable Haemophilus influenzae major outer membrane protein P5	0:66	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	3	29	theme	bacterial	516:524	arg1	resistance					532:541	bacterial serum resistance	516:541	bacterial serum resistance	516:541	We previously showed that the outer membrane protein P5 contributes to bacterial serum resistance by the recruitment of complement regulators.					
37305414	10	30	theme	airway	1426:1431	arg1	cells					1444:1448	airway epithelial cells	1426:1448	airway epithelial cells	1426:1448	We found (i) a decreased adherence to airway epithelial cells and fibronectin, (ii) increased complement-mediated killing, and (iii) increased sensitivity to the β-lactam antibiotics in both mutants compared to NTHi 3655 wild-type.					
37305414	10	31	theme	NTHi	1599:1602	arg1	wild-type					1609:1617	NTHi 3655 wild-type	1599:1617	NTHi 3655 wild-type	1599:1617	We found (i) a decreased adherence to airway epithelial cells and fibronectin, (ii) increased complement-mediated killing, and (iii) increased sensitivity to the β-lactam antibiotics in both mutants compared to NTHi 3655 wild-type.					
37305414	0	32	theme	Non-typeable	0:11	arg1	P5					65:66	Non-typeable Haemophilus influenzae major outer membrane protein P5	0:66	Non-typeable Haemophilus influenzae major outer membrane protein P5	0:66	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	9	33	theme	attenuated	1307:1316	arg1	phenotypes					1329:1338	similar attenuated pathogenic phenotypes	1299:1338	similar attenuated pathogenic phenotypes observed in both NTHi 3655Δp5 and NTHi 3655Δp5	1299:1385	This was also supported by similar attenuated pathogenic phenotypes observed in both NTHi 3655Δp5 and NTHi 3655Δp5.					
37305414	1	34	theme	wide	295:298	arg1	range					300:304	a wide range	293:304	a wide range of airway diseases	293:323	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	12	35	theme	bacterial	1819:1827	arg1	stability					1832:1840	bacterial OM stability	1819:1840	bacterial OM stability	1819:1840	In conclusion, our results suggest that P5 is important for bacterial OM stability, which ultimately affects the membrane proteome and NTHi pathogenesis.					
37305414	6	36	theme	peptidoglycan-binding	853:873	arg1	assay					875:879	a peptidoglycan-binding assay	851:879	a peptidoglycan-binding assay	851:879	In a peptidoglycan-binding assay, the CTD of P5 (P5CTD) formed a complex with peptidoglycan.					
37305414	8	37	theme	airway	1222:1227	arg1	mucosa					1229:1234	the airway mucosa	1218:1234	the airway mucosa	1218:1234	Relative abundance of several membrane-associated virulence factors that are crucial for adherence to the airway mucosa, and serum resistance were altered.					
37305414	0	38	theme	outer	42:46	arg1	P5					65:66	Non-typeable Haemophilus influenzae major outer membrane protein P5	0:66	Non-typeable Haemophilus influenzae major outer membrane protein P5	0:66	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	10	39	theme	complement-mediated	1482:1500	arg1	killing					1502:1508	complement-mediated killing	1482:1508	complement-mediated killing	1482:1508	We found (i) a decreased adherence to airway epithelial cells and fibronectin, (ii) increased complement-mediated killing, and (iii) increased sensitivity to the β-lactam antibiotics in both mutants compared to NTHi 3655 wild-type.					
37305414	3	40	theme	membrane	481:488	arg1	P5					498:499	the outer membrane protein P5	471:499	the outer membrane protein P5	471:499	We previously showed that the outer membrane protein P5 contributes to bacterial serum resistance by the recruitment of complement regulators.					
37305414	0	41	theme	protein	57:63	arg1	P5					65:66	Non-typeable Haemophilus influenzae major outer membrane protein P5	0:66	Non-typeable Haemophilus influenzae major outer membrane protein P5	0:66	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	9	42	theme	NTHi	1357:1360	arg1	3655Δp5					1362:1368	NTHi 3655Δp5	1357:1368	NTHi 3655Δp5	1357:1368	This was also supported by similar attenuated pathogenic phenotypes observed in both NTHi 3655Δp5 and NTHi 3655Δp5.					
37305414	12	43	theme	OM	1829:1830	arg1	stability					1832:1840	bacterial OM stability	1819:1840	bacterial OM stability	1819:1840	In conclusion, our results suggest that P5 is important for bacterial OM stability, which ultimately affects the membrane proteome and NTHi pathogenesis.					
37305414	4	44	theme	novel	606:610	arg1	role					612:615	a novel role	604:615	a novel role of P5 in maintaining bacterial outer membrane (OM) integrity	604:676	Here, we report a novel role of P5 in maintaining bacterial outer membrane (OM) integrity and protein composition important for NTHi-host interactions.					
37305414	0	45	theme	human	192:196	arg1	host					198:201	the human host	188:201	the human host	188:201	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	2	46	theme	mechanisms	349:358	arg1	plethora					337:344	a plethora	335:344	a plethora of mechanisms	335:358	NTHi has a plethora of mechanisms to colonize while evading the host immune system for the establishment of infection.					
37305414	1	47	theme	Gram-negative	252:264	arg1	influenzae					229:238	Non-typeable Haemophilus influenzae	204:238	Non-typeable Haemophilus influenzae (NTHi)	204:245	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	1	47	theme	Gram-negative	252:264	arg1	pathogen					272:279	a Gram-negative human pathogen	250:279	a Gram-negative human pathogen that causes a wide range of airway diseases	250:323	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	6	48	theme	P5	893:894	arg1	CTD					886:888	the CTD	882:888	the CTD of P5 (P5CTD)	882:902	In a peptidoglycan-binding assay, the CTD of P5 (P5CTD) formed a complex with peptidoglycan.					
37305414	7	49	theme	strains	1063:1069	arg1	composition					1044:1054	the membrane protein composition	1023:1054	the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5	1023:1099	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	3	50	theme	serum	526:530	arg1	resistance					532:541	bacterial serum resistance	516:541	bacterial serum resistance	516:541	We previously showed that the outer membrane protein P5 contributes to bacterial serum resistance by the recruitment of complement regulators.					
37305414	8	51	theme	several	1138:1144	arg1	factors					1176:1182	several membrane-associated virulence factors	1138:1182	several membrane-associated virulence factors that are crucial for adherence to the airway mucosa	1138:1234	Relative abundance of several membrane-associated virulence factors that are crucial for adherence to the airway mucosa, and serum resistance were altered.					
37305414	4	52	theme	bacterial	638:646	arg1	OM					664:665	OM	664:665	OM	664:665	Here, we report a novel role of P5 in maintaining bacterial outer membrane (OM) integrity and protein composition important for NTHi-host interactions.					
37305414	4	52	theme	bacterial	638:646	arg1	membrane					654:661	bacterial outer membrane	638:661	bacterial outer membrane (OM) integrity	638:676	Here, we report a novel role of P5 in maintaining bacterial outer membrane (OM) integrity and protein composition important for NTHi-host interactions.					
37305414	10	53	dep	increased	1472:1480	arg1	ii					1468:1469	ii	1468:1469	ii	1468:1469	We found (i) a decreased adherence to airway epithelial cells and fibronectin, (ii) increased complement-mediated killing, and (iii) increased sensitivity to the β-lactam antibiotics in both mutants compared to NTHi 3655 wild-type.					
37305414	3	54	theme	complement	565:574	arg1	regulators					576:585	complement regulators	565:585	complement regulators	565:585	We previously showed that the outer membrane protein P5 contributes to bacterial serum resistance by the recruitment of complement regulators.					
37305414	8	55	theme	virulence	1166:1174	arg1	factors					1176:1182	several membrane-associated virulence factors	1138:1182	several membrane-associated virulence factors that are crucial for adherence to the airway mucosa	1138:1234	Relative abundance of several membrane-associated virulence factors that are crucial for adherence to the airway mucosa, and serum resistance were altered.					
37305414	4	56	theme	membrane	654:661	arg1	integrity					668:676	bacterial outer membrane (OM) integrity	638:676	bacterial outer membrane (OM) integrity	638:676	Here, we report a novel role of P5 in maintaining bacterial outer membrane (OM) integrity and protein composition important for NTHi-host interactions.					
37305414	7	57	theme	protein	1036:1042	arg1	composition					1044:1054	the membrane protein composition	1023:1054	the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5	1023:1099	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	1	58	theme	Non-typeable	204:215	arg1	pathogen					272:279	a Gram-negative human pathogen	250:279	a Gram-negative human pathogen that causes a wide range of airway diseases	250:323	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	1	58	theme	Non-typeable	204:215	arg1	NTHi					241:244	NTHi	241:244	NTHi	241:244	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	1	58	theme	Non-typeable	204:215	arg1	influenzae					229:238	Non-typeable Haemophilus influenzae	204:238	Non-typeable Haemophilus influenzae (NTHi)	204:245	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	7	59	dep	strains	1063:1069	arg1	strains					1063:1069	the strains NTHi 3655Δp5 and NTHi 3655Δp5	1059:1099	the strains NTHi 3655Δp5 and NTHi 3655Δp5	1059:1099	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	7	59	dep	strains	1063:1069	arg1	3655Δp5					1076:1082	NTHi 3655Δp5	1071:1082	NTHi 3655Δp5	1071:1082	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	7	59	dep	strains	1063:1069	arg1	3655Δp5					1093:1099	NTHi 3655Δp5	1088:1099	NTHi 3655Δp5	1088:1099	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	7	60	theme	P5	1012:1013	arg1	deletion					982:989	deletion	982:989	deletion of CTD or the entire P5	982:1013	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	0	61	theme	membrane	129:136	arg1	composition					146:156	the membrane protein composition	125:156	the membrane protein composition crucial for interactions with the human host	125:201	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	5	62	dep	In	740:741	arg1	silico					743:748	silico	743:748	silico	743:748	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	9	63	located	observed	1340:1347	arg1	3655Δp5					1379:1385	NTHi 3655Δp5	1374:1385	NTHi 3655Δp5	1374:1385	This was also supported by similar attenuated pathogenic phenotypes observed in both NTHi 3655Δp5 and NTHi 3655Δp5.					
37305414	9	63	located	observed	1340:1347	arg1	3655Δp5					1362:1368	NTHi 3655Δp5	1357:1368	NTHi 3655Δp5	1357:1368	This was also supported by similar attenuated pathogenic phenotypes observed in both NTHi 3655Δp5 and NTHi 3655Δp5.					
37305414	9	63	located	observed	1340:1347	arg2	phenotypes					1329:1338	similar attenuated pathogenic phenotypes	1299:1338	similar attenuated pathogenic phenotypes observed in both NTHi 3655Δp5 and NTHi 3655Δp5	1299:1385	This was also supported by similar attenuated pathogenic phenotypes observed in both NTHi 3655Δp5 and NTHi 3655Δp5.					
37305414	4	64	theme	protein	682:688	arg1	composition					690:700	protein composition	682:700	protein composition	682:700	Here, we report a novel role of P5 in maintaining bacterial outer membrane (OM) integrity and protein composition important for NTHi-host interactions.					
37305414	4	65	theme	important	702:710	arg1	role					612:615	a novel role	604:615	a novel role of P5 in maintaining bacterial outer membrane (OM) integrity	604:676	Here, we report a novel role of P5 in maintaining bacterial outer membrane (OM) integrity and protein composition important for NTHi-host interactions.					
37305414	4	66	theme	P5	620:621	arg1	composition					690:700	protein composition	682:700	protein composition	682:700	Here, we report a novel role of P5 in maintaining bacterial outer membrane (OM) integrity and protein composition important for NTHi-host interactions.					
37305414	4	66	theme	P5	620:621	arg1	role					612:615	a novel role	604:615	a novel role of P5 in maintaining bacterial outer membrane (OM) integrity	604:676	Here, we report a novel role of P5 in maintaining bacterial outer membrane (OM) integrity and protein composition important for NTHi-host interactions.					
37305414	0	67	theme	membrane	93:100	arg1	stability					102:110	bacterial membrane stability	83:110	bacterial membrane stability	83:110	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	2	68	contain	has	331:333	arg1	NTHi					326:329	NTHi	326:329	NTHi	326:329	NTHi has a plethora of mechanisms to colonize while evading the host immune system for the establishment of infection.					
37305414	2	68	contain	has	331:333	arg2	plethora					337:344	a plethora	335:344	a plethora of mechanisms	335:358	NTHi has a plethora of mechanisms to colonize while evading the host immune system for the establishment of infection.					
37305414	11	69	from	conditions	1684:1693	arg1	sensitive					1649:1657	sensitive	1649:1657	sensitive	1649:1657	These mutants were also more sensitive to lysis at hyperosmotic conditions and hypervesiculated compared to the parent wild-type bacteria.					
37305414	10	70	theme	decreased	1403:1411	arg1	adherence					1413:1421	a decreased adherence	1401:1421	(i) a decreased adherence to airway epithelial cells	1397:1448	We found (i) a decreased adherence to airway epithelial cells and fibronectin, (ii) increased complement-mediated killing, and (iii) increased sensitivity to the β-lactam antibiotics in both mutants compared to NTHi 3655 wild-type.					
37305414	12	71	theme	NTHi	1894:1897	arg1	pathogenesis					1899:1910	NTHi pathogenesis	1894:1910	NTHi pathogenesis	1894:1910	In conclusion, our results suggest that P5 is important for bacterial OM stability, which ultimately affects the membrane proteome and NTHi pathogenesis.					
37305414	7	72	theme	Protein	941:947	arg1	analysis					959:966	Protein profiling analysis	941:966	Protein profiling analysis	941:966	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	11	73	theme	parent	1732:1737	arg1	bacteria					1749:1756	the parent wild-type bacteria	1728:1756	the parent wild-type bacteria	1728:1756	These mutants were also more sensitive to lysis at hyperosmotic conditions and hypervesiculated compared to the parent wild-type bacteria.					
37305414	10	74	theme	epithelial	1433:1442	arg1	cells					1444:1448	airway epithelial cells	1426:1448	airway epithelial cells	1426:1448	We found (i) a decreased adherence to airway epithelial cells and fibronectin, (ii) increased complement-mediated killing, and (iii) increased sensitivity to the β-lactam antibiotics in both mutants compared to NTHi 3655 wild-type.					
37305414	10	75	theme	3655	1604:1607	arg1	wild-type					1609:1617	NTHi 3655 wild-type	1599:1617	NTHi 3655 wild-type	1599:1617	We found (i) a decreased adherence to airway epithelial cells and fibronectin, (ii) increased complement-mediated killing, and (iii) increased sensitivity to the β-lactam antibiotics in both mutants compared to NTHi 3655 wild-type.					
37305414	5	76	theme	periplasmic	805:815	arg1	CTD					836:838	CTD	836:838	CTD	836:838	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	5	76	theme	periplasmic	805:815	arg1	P5					844:845	P5	844:845	P5	844:845	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	5	76	theme	periplasmic	805:815	arg1	domain					828:833	the periplasmic C-terminal domain	801:833	the periplasmic C-terminal domain (CTD) of P5	801:845	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	9	77	theme	similar	1299:1305	arg1	phenotypes					1329:1338	similar attenuated pathogenic phenotypes	1299:1338	similar attenuated pathogenic phenotypes observed in both NTHi 3655Δp5 and NTHi 3655Δp5	1299:1385	This was also supported by similar attenuated pathogenic phenotypes observed in both NTHi 3655Δp5 and NTHi 3655Δp5.					
37305414	2	78	theme	infection	434:442	arg1	establishment					417:429	the establishment	413:429	the establishment of infection	413:442	NTHi has a plethora of mechanisms to colonize while evading the host immune system for the establishment of infection.					
37305414	4	79	theme	NTHi-host	716:724	arg1	interactions					726:737	NTHi-host interactions	716:737	NTHi-host interactions	716:737	Here, we report a novel role of P5 in maintaining bacterial outer membrane (OM) integrity and protein composition important for NTHi-host interactions.					
37305414	0	80	theme	major	36:40	arg1	P5					65:66	Non-typeable Haemophilus influenzae major outer membrane protein P5	0:66	Non-typeable Haemophilus influenzae major outer membrane protein P5	0:66	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	9	81	theme	pathogenic	1318:1327	arg1	phenotypes					1329:1338	similar attenuated pathogenic phenotypes	1299:1338	similar attenuated pathogenic phenotypes observed in both NTHi 3655Δp5 and NTHi 3655Δp5	1299:1385	This was also supported by similar attenuated pathogenic phenotypes observed in both NTHi 3655Δp5 and NTHi 3655Δp5.					
37305414	7	82	theme	NTHi	1088:1091	arg1	strains					1063:1069	the strains NTHi 3655Δp5 and NTHi 3655Δp5	1059:1099	the strains NTHi 3655Δp5 and NTHi 3655Δp5	1059:1099	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	7	82	theme	NTHi	1088:1091	arg1	3655Δp5					1093:1099	NTHi 3655Δp5	1088:1099	NTHi 3655Δp5	1088:1099	Protein profiling analysis revealed that deletion of CTD or the entire P5 changed the membrane protein composition of the strains NTHi 3655Δp5 and NTHi 3655Δp5, respectively.					
37305414	5	83	theme	peptidoglycan-binding	770:790	arg1	motif					792:796	a peptidoglycan-binding motif	768:796	a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5	768:845	In silico analysis revealed a peptidoglycan-binding motif at the periplasmic C-terminal domain (CTD) of P5.					
37305414	2	84	theme	immune	395:400	arg1	system					402:407	the host immune system	386:407	the host immune system	386:407	NTHi has a plethora of mechanisms to colonize while evading the host immune system for the establishment of infection.					
37305414	10	85	dep	adherence	1413:1421	arg1	i					1398:1398	i	1398:1398	i	1398:1398	We found (i) a decreased adherence to airway epithelial cells and fibronectin, (ii) increased complement-mediated killing, and (iii) increased sensitivity to the β-lactam antibiotics in both mutants compared to NTHi 3655 wild-type.					
37305414	1	86	theme	airway	309:314	arg1	diseases					316:323	airway diseases	309:323	airway diseases	309:323	Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes a wide range of airway diseases.					
37305414	0	87	theme	protein	138:144	arg1	composition					146:156	the membrane protein composition	125:156	the membrane protein composition crucial for interactions with the human host	125:201	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	11	88	theme	hyperosmotic	1671:1682	arg1	conditions					1684:1693	hyperosmotic conditions	1671:1693	hyperosmotic conditions	1671:1693	These mutants were also more sensitive to lysis at hyperosmotic conditions and hypervesiculated compared to the parent wild-type bacteria.					
37305414	3	89	theme	protein	490:496	arg1	P5					498:499	the outer membrane protein P5	471:499	the outer membrane protein P5	471:499	We previously showed that the outer membrane protein P5 contributes to bacterial serum resistance by the recruitment of complement regulators.					
37305414	3	90	theme	outer	475:479	arg1	P5					498:499	the outer membrane protein P5	471:499	the outer membrane protein P5	471:499	We previously showed that the outer membrane protein P5 contributes to bacterial serum resistance by the recruitment of complement regulators.					
37305414	9	91	theme	NTHi	1374:1377	arg1	3655Δp5					1379:1385	NTHi 3655Δp5	1374:1385	NTHi 3655Δp5	1374:1385	This was also supported by similar attenuated pathogenic phenotypes observed in both NTHi 3655Δp5 and NTHi 3655Δp5.					
37305414	0	92	dep	Haemophilus	13:23	arg1	influenzae					25:34	influenzae	25:34	influenzae	25:34	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
37305414	0	93	theme	Haemophilus	13:23	arg1	P5					65:66	Non-typeable Haemophilus influenzae major outer membrane protein P5	0:66	Non-typeable Haemophilus influenzae major outer membrane protein P5	0:66	Non-typeable Haemophilus influenzae major outer membrane protein P5 contributes to bacterial membrane stability, and affects the membrane protein composition crucial for interactions with the human host.					
36723743	4	0	theme	poloxamer	560:568	arg1	use					548:550	the use	544:550	the use of pure poloxamer	544:568	Compared with the use of pure poloxamer, the chitosan complex demonstrated improved mucoadhesion in in vitro studies, and the phase transition parameters were optimal for intranasal administration.					
36723743	4	1	theme	pure	555:558	arg1	poloxamer					560:568	pure poloxamer	555:568	pure poloxamer	555:568	Compared with the use of pure poloxamer, the chitosan complex demonstrated improved mucoadhesion in in vitro studies, and the phase transition parameters were optimal for intranasal administration.					
36723743	2	2	theme	chitosan	225:232	arg1	formate					234:240	chitosan formate	225:240	chitosan formate exhibiting the highest pH-sensitivity	225:278	Among common chitosan salts obtained by heterogeneous synthesis, chitosan formate exhibiting the highest pH-sensitivity was selected.					
36723743	3	3	theme	composition	340:350	arg1	pH					369:370	high pH	364:370	high pH required for the phase transition	364:404	However, the low mucoadhesion strength of the composition, as well as high pH required for the phase transition necessitate designing a polycomponent compound with a poloxamer to additionally provide a thermosensitive phase transition.					
36723743	3	3	theme	composition	340:350	arg1	strength					324:331	the low mucoadhesion strength	303:331	the low mucoadhesion strength of the composition	303:350	However, the low mucoadhesion strength of the composition, as well as high pH required for the phase transition necessitate designing a polycomponent compound with a poloxamer to additionally provide a thermosensitive phase transition.					
36723743	4	4	dep	demonstrated	592:603	arg1	improved					605:612	improved	605:612	demonstrated improved mucoadhesion in in vitro studies	592:645	Compared with the use of pure poloxamer, the chitosan complex demonstrated improved mucoadhesion in in vitro studies, and the phase transition parameters were optimal for intranasal administration.					
36723743	2	5	theme	highest	257:263	arg1	pH-sensitivity					265:278	the highest pH-sensitivity	253:278	the highest pH-sensitivity	253:278	Among common chitosan salts obtained by heterogeneous synthesis, chitosan formate exhibiting the highest pH-sensitivity was selected.					
36723743	4	6	theme	intranasal	701:710	arg1	administration					712:725	intranasal administration	701:725	intranasal administration	701:725	Compared with the use of pure poloxamer, the chitosan complex demonstrated improved mucoadhesion in in vitro studies, and the phase transition parameters were optimal for intranasal administration.					
36723743	5	7	theme	in	731:732	arg1	study					739:743	An in vivo study	728:743	An in vivo study	728:743	An in vivo study revealed no locally irritating effect of the composition, which allows its further development.					
36723743	3	8	theme	phase	512:516	arg1	transition					518:527	a thermosensitive phase transition	494:527	a thermosensitive phase transition	494:527	However, the low mucoadhesion strength of the composition, as well as high pH required for the phase transition necessitate designing a polycomponent compound with a poloxamer to additionally provide a thermosensitive phase transition.					
36723743	1	9	theme	pH-sensitive	131:142	arg1	matrix					152:157	a pH-sensitive in situ matrix	129:157	a pH-sensitive in situ matrix	129:157	We studied the potential of using a salt form of chitosan as a pH-sensitive in situ matrix.					
36723743	1	9	theme	pH-sensitive	131:142	arg1	form					109:112	a salt form	102:112	a salt form of chitosan	102:124	We studied the potential of using a salt form of chitosan as a pH-sensitive in situ matrix.					
36723743	5	10	theme	irritating	765:774	arg1	effect					776:781	no locally irritating effect	754:781	no locally irritating effect	754:781	An in vivo study revealed no locally irritating effect of the composition, which allows its further development.					
36723743	1	11	theme	in	144:145	arg1	matrix					152:157	a pH-sensitive in situ matrix	129:157	a pH-sensitive in situ matrix	129:157	We studied the potential of using a salt form of chitosan as a pH-sensitive in situ matrix.					
36723743	1	11	theme	in	144:145	arg1	form					109:112	a salt form	102:112	a salt form of chitosan	102:124	We studied the potential of using a salt form of chitosan as a pH-sensitive in situ matrix.					
36723743	5	12	theme	composition	790:800	arg1	effect					776:781	no locally irritating effect	754:781	no locally irritating effect	754:781	An in vivo study revealed no locally irritating effect of the composition, which allows its further development.					
36723743	3	13	theme	phase	389:393	arg1	transition					395:404	the phase transition	385:404	the phase transition	385:404	However, the low mucoadhesion strength of the composition, as well as high pH required for the phase transition necessitate designing a polycomponent compound with a poloxamer to additionally provide a thermosensitive phase transition.					
36723743	3	14	theme	polycomponent	430:442	arg1	compound					444:451	a polycomponent compound	428:451	a polycomponent compound with a poloxamer to additionally provide a thermosensitive phase transition	428:527	However, the low mucoadhesion strength of the composition, as well as high pH required for the phase transition necessitate designing a polycomponent compound with a poloxamer to additionally provide a thermosensitive phase transition.					
36723743	0	15	theme	In	15:16	arg1	System					34:39	In Situ Intranasal System	15:39	In Situ Intranasal System	15:39	Development of In Situ Intranasal System Based on Chitosan Formate.					
36723743	5	16	theme	further	820:826	arg1	development					828:838	its further development	816:838	its further development	816:838	An in vivo study revealed no locally irritating effect of the composition, which allows its further development.					
36723743	0	17	theme	System	34:39	arg1	Development					0:10	Development	0:10	Development of In Situ Intranasal System	0:39	Development of In Situ Intranasal System Based on Chitosan Formate.					
36723743	2	18	theme	heterogeneous	200:212	arg1	synthesis					214:222	heterogeneous synthesis	200:222	heterogeneous synthesis	200:222	Among common chitosan salts obtained by heterogeneous synthesis, chitosan formate exhibiting the highest pH-sensitivity was selected.					
36723743	0	19	theme	Intranasal	23:32	arg1	System					34:39	In Situ Intranasal System	15:39	In Situ Intranasal System	15:39	Development of In Situ Intranasal System Based on Chitosan Formate.					
36723743	3	20	with	compound	444:451	arg1	poloxamer					460:468	a poloxamer to additionally provide a thermosensitive phase transition	458:527	a poloxamer to additionally provide a thermosensitive phase transition	458:527	However, the low mucoadhesion strength of the composition, as well as high pH required for the phase transition necessitate designing a polycomponent compound with a poloxamer to additionally provide a thermosensitive phase transition.					
36723743	4	21	dep	in	630:631	arg1	vitro					633:637	vitro	633:637	vitro	633:637	Compared with the use of pure poloxamer, the chitosan complex demonstrated improved mucoadhesion in in vitro studies, and the phase transition parameters were optimal for intranasal administration.					
36723743	4	22	theme	in	630:631	arg1	studies					639:645	in vitro studies	630:645	in vitro studies	630:645	Compared with the use of pure poloxamer, the chitosan complex demonstrated improved mucoadhesion in in vitro studies, and the phase transition parameters were optimal for intranasal administration.					
36723743	3	23	theme	high	364:367	arg1	pH					369:370	high pH	364:370	high pH required for the phase transition	364:404	However, the low mucoadhesion strength of the composition, as well as high pH required for the phase transition necessitate designing a polycomponent compound with a poloxamer to additionally provide a thermosensitive phase transition.					
36723743	5	24	dep	in	731:732	arg1	vivo					734:737	vivo	734:737	vivo	734:737	An in vivo study revealed no locally irritating effect of the composition, which allows its further development.					
36723743	0	25	theme	Chitosan	50:57	arg1	Formate					59:65	Chitosan Formate	50:65	Chitosan Formate	50:65	Development of In Situ Intranasal System Based on Chitosan Formate.					
36723743	2	26	theme	chitosan	173:180	arg1	salts					182:186	common chitosan salts	166:186	common chitosan salts obtained by heterogeneous synthesis	166:222	Among common chitosan salts obtained by heterogeneous synthesis, chitosan formate exhibiting the highest pH-sensitivity was selected.					
36723743	1	27	dep	in	144:145	arg1	situ					147:150	situ	147:150	situ	147:150	We studied the potential of using a salt form of chitosan as a pH-sensitive in situ matrix.					
36723743	2	28	theme	common	166:171	arg1	salts					182:186	common chitosan salts	166:186	common chitosan salts obtained by heterogeneous synthesis	166:222	Among common chitosan salts obtained by heterogeneous synthesis, chitosan formate exhibiting the highest pH-sensitivity was selected.					
36723743	4	29	theme	phase	656:660	arg1	parameters					673:682	the phase transition parameters	652:682	the phase transition parameters	652:682	Compared with the use of pure poloxamer, the chitosan complex demonstrated improved mucoadhesion in in vitro studies, and the phase transition parameters were optimal for intranasal administration.					
36723743	4	29	theme	phase	656:660	arg1	optimal					689:695	optimal	689:695	optimal	689:695	Compared with the use of pure poloxamer, the chitosan complex demonstrated improved mucoadhesion in in vitro studies, and the phase transition parameters were optimal for intranasal administration.					
36723743	1	30	theme	salt	104:107	arg1	form					109:112	a salt form	102:112	a salt form of chitosan	102:124	We studied the potential of using a salt form of chitosan as a pH-sensitive in situ matrix.					
36723743	1	30	theme	salt	104:107	arg1	matrix					152:157	a pH-sensitive in situ matrix	129:157	a pH-sensitive in situ matrix	129:157	We studied the potential of using a salt form of chitosan as a pH-sensitive in situ matrix.					
36723743	0	31	dep	In	15:16	arg1	Situ					18:21	Situ	18:21	Situ	18:21	Development of In Situ Intranasal System Based on Chitosan Formate.					
36723743	1	32	theme	chitosan	117:124	arg1	form					109:112	a salt form	102:112	a salt form of chitosan	102:124	We studied the potential of using a salt form of chitosan as a pH-sensitive in situ matrix.					
36723743	1	32	theme	chitosan	117:124	arg1	matrix					152:157	a pH-sensitive in situ matrix	129:157	a pH-sensitive in situ matrix	129:157	We studied the potential of using a salt form of chitosan as a pH-sensitive in situ matrix.					
36723743	4	33	theme	chitosan	575:582	arg1	complex					584:590	the chitosan complex	571:590	the chitosan complex	571:590	Compared with the use of pure poloxamer, the chitosan complex demonstrated improved mucoadhesion in in vitro studies, and the phase transition parameters were optimal for intranasal administration.					
36723743	3	34	theme	low	307:309	arg1	strength					324:331	the low mucoadhesion strength	303:331	the low mucoadhesion strength of the composition	303:350	However, the low mucoadhesion strength of the composition, as well as high pH required for the phase transition necessitate designing a polycomponent compound with a poloxamer to additionally provide a thermosensitive phase transition.					
36723743	4	35	theme	transition	662:671	arg1	parameters					673:682	the phase transition parameters	652:682	the phase transition parameters	652:682	Compared with the use of pure poloxamer, the chitosan complex demonstrated improved mucoadhesion in in vitro studies, and the phase transition parameters were optimal for intranasal administration.					
36723743	4	35	theme	transition	662:671	arg1	optimal					689:695	optimal	689:695	optimal	689:695	Compared with the use of pure poloxamer, the chitosan complex demonstrated improved mucoadhesion in in vitro studies, and the phase transition parameters were optimal for intranasal administration.					
36723743	3	36	theme	mucoadhesion	311:322	arg1	strength					324:331	the low mucoadhesion strength	303:331	the low mucoadhesion strength of the composition	303:350	However, the low mucoadhesion strength of the composition, as well as high pH required for the phase transition necessitate designing a polycomponent compound with a poloxamer to additionally provide a thermosensitive phase transition.					
36723743	3	37	theme	thermosensitive	496:510	arg1	transition					518:527	a thermosensitive phase transition	494:527	a thermosensitive phase transition	494:527	However, the low mucoadhesion strength of the composition, as well as high pH required for the phase transition necessitate designing a polycomponent compound with a poloxamer to additionally provide a thermosensitive phase transition.					
36513176	0	0	theme	film	99:102	arg1	properties					49:58	The physiochemical and photodynamic inactivation properties	0:58	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.	0:144	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	4	1	theme	26.36 μg/mL	807:817	arg1	peroxide					828:835	26.36 μg/mL hydrogen peroxide	807:835	26.36 μg/mL hydrogen peroxide	807:835	The results showed that the CS-EB films stimulated a maximum of 26.36 μg/mL hydrogen peroxide and 74.5 μg/g hydroxyl radical under irradiation.					
36513176	7	2	theme	material	1380:1387	arg1	type					1337:1340	a new type	1331:1340	a new type of environmentally friendly packaging material	1331:1387	Overall, the CS-EB composite films can inhibit the growth of bacteria through photodynamic inactivation and has the potential to become a new type of environmentally friendly packaging material.					
36513176	4	3	theme	74.5 μg/g	841:849	arg1	radical					860:866	74.5 μg/g hydroxyl radical	841:866	74.5 μg/g hydroxyl radical	841:866	The results showed that the CS-EB films stimulated a maximum of 26.36 μg/mL hydrogen peroxide and 74.5 μg/g hydroxyl radical under irradiation.					
36513176	7	4	contain	has	1303:1305	arg1	films					1224:1228	the CS-EB composite films	1204:1228	the CS-EB composite films	1204:1228	Overall, the CS-EB composite films can inhibit the growth of bacteria through photodynamic inactivation and has the potential to become a new type of environmentally friendly packaging material.					
36513176	7	4	contain	has	1303:1305	arg2	potential					1311:1319	the potential to become a new type of environmentally friendly packaging material	1307:1387	the potential to become a new type of environmentally friendly packaging material	1307:1387	Overall, the CS-EB composite films can inhibit the growth of bacteria through photodynamic inactivation and has the potential to become a new type of environmentally friendly packaging material.					
36513176	0	5	theme	composite	89:97	arg1	film					99:102	corn starch/erythrosine B composite film	63:102	corn starch/erythrosine B composite film	63:102	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	1	6	theme	CS	236:237	arg1	film					240:243	corn starch (CS) film	223:243	corn starch (CS) film	223:243	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	5	7	theme	pork	1055:1058	arg1	samples					1060:1066	the pork samples	1051:1066	the pork samples	1051:1066	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	2	8	theme	moisture	558:565	arg1	content					567:573	moisture content	558:573	moisture content	558:573	The study revealed that the physiochemical properties of CS films: moisture content, water solubility, and water vapor transmission were improved with the addition of EB.					
36513176	7	9	theme	environmentally	1345:1359	arg1	material					1380:1387	environmentally friendly packaging material	1345:1387	environmentally friendly packaging material	1345:1387	Overall, the CS-EB composite films can inhibit the growth of bacteria through photodynamic inactivation and has the potential to become a new type of environmentally friendly packaging material.					
36513176	4	10	theme	hydrogen	819:826	arg1	peroxide					828:835	26.36 μg/mL hydrogen peroxide	807:835	26.36 μg/mL hydrogen peroxide	807:835	The results showed that the CS-EB films stimulated a maximum of 26.36 μg/mL hydrogen peroxide and 74.5 μg/g hydroxyl radical under irradiation.					
36513176	2	11	theme	vapor	604:608	arg1	transmission					610:621	water vapor transmission	598:621	water vapor transmission	598:621	The study revealed that the physiochemical properties of CS films: moisture content, water solubility, and water vapor transmission were improved with the addition of EB.					
36513176	5	12	theme	5 	917:918	arg1	%					919:919	%	919:919	%	919:919	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	2	13	theme	water	598:602	arg1	transmission					610:621	water vapor transmission	598:621	water vapor transmission	598:621	The study revealed that the physiochemical properties of CS films: moisture content, water solubility, and water vapor transmission were improved with the addition of EB.					
36513176	5	14	theme	%	919:919	arg1	concentration					921:933	a 5 % concentration	915:933	a 5 % concentration of EB	915:939	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	6	15	theme	antibacterial	1121:1133	arg1	ability					1135:1141	the antibacterial ability	1117:1141	the antibacterial ability	1117:1141	Moreover, the antibacterial ability was enhanced with the increase in EB concentration.					
36513176	0	16	theme	application	112:122	arg1	properties					49:58	The physiochemical and photodynamic inactivation properties	0:58	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.	0:144	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	0	17	from	properties	49:58	arg1	preservation					132:143	pork preservation	127:143	pork preservation	127:143	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	6	18	theme	EB	1177:1178	arg1	concentration					1180:1192	EB concentration	1177:1192	EB concentration	1177:1192	Moreover, the antibacterial ability was enhanced with the increase in EB concentration.					
36513176	1	19	theme	D65	446:448	arg1	400-800 nm					478:487	400-800 nm	478:487	400-800 nm	478:487	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	1	19	theme	D65	446:448	arg1	diode					465:469	D65 light-emitting diode	446:469	D65 light-emitting diode (LED) (400-800 nm)	446:488	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	1	20	theme	erythrosine	180:190	arg1	B					192:192	erythrosine B	180:192	erythrosine B (EB)	180:197	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	1	20	theme	erythrosine	180:190	arg1	EB					195:196	EB	195:196	EB	195:196	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	0	21	theme	physiochemical	4:17	arg1	properties					49:58	The physiochemical and photodynamic inactivation properties	0:58	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.	0:144	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	5	22	theme	Log	1037:1039	arg1	CFU/mL					1041:1046	2.4 Log CFU/mL	1033:1046	2.4 Log CFU/mL on the pork samples	1033:1066	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	1	23	theme	light-emitting	450:463	arg1	400-800 nm					478:487	400-800 nm	478:487	400-800 nm	478:487	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	1	23	theme	light-emitting	450:463	arg1	diode					465:469	D65 light-emitting diode	446:469	D65 light-emitting diode (LED) (400-800 nm)	446:488	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	5	24	theme	CS	891:892	arg1	films					904:908	The CS composite films	887:908	The CS composite films with a 5 % concentration of EB	887:939	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	1	25	theme	B	192:192	arg1	photosensitizer					204:218	a photosensitizer	202:218	a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm)	202:488	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	1	25	theme	B	192:192	arg1	effect					170:175	the effect	166:175	the effect of erythrosine B (EB)	166:197	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	3	26	theme	thermal	698:704	arg1	stability					706:714	the thermal stability	694:714	the thermal stability	694:714	In addition, the elasticity and the thermal stability of the film were enhanced.					
36513176	2	27	theme	water	576:580	arg1	solubility					582:591	water solubility	576:591	water solubility	576:591	The study revealed that the physiochemical properties of CS films: moisture content, water solubility, and water vapor transmission were improved with the addition of EB.					
36513176	1	28	theme	diode	465:469	arg1	irradiation					431:441	the irradiation	427:441	the irradiation of D65 light-emitting diode (LED) (400-800 nm)	427:488	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	5	29	theme	composite	894:902	arg1	films					904:908	The CS composite films	887:908	The CS composite films with a 5 % concentration of EB	887:939	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	7	30	theme	packaging	1370:1378	arg1	material					1380:1387	environmentally friendly packaging material	1345:1387	environmentally friendly packaging material	1345:1387	Overall, the CS-EB composite films can inhibit the growth of bacteria through photodynamic inactivation and has the potential to become a new type of environmentally friendly packaging material.					
36513176	0	31	theme	photodynamic	23:34	arg1	properties					49:58	The physiochemical and photodynamic inactivation properties	0:58	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.	0:144	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	2	32	theme	physiochemical	519:532	arg1	properties					534:543	the physiochemical properties	515:543	the physiochemical properties of CS films	515:555	The study revealed that the physiochemical properties of CS films: moisture content, water solubility, and water vapor transmission were improved with the addition of EB.					
36513176	5	33	from	CFU/mL	983:988	arg1	samples					1060:1066	the pork samples	1051:1066	the pork samples	1051:1066	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	7	34	theme	new	1333:1335	arg1	type					1337:1340	a new type	1331:1340	a new type of environmentally friendly packaging material	1331:1387	Overall, the CS-EB composite films can inhibit the growth of bacteria through photodynamic inactivation and has the potential to become a new type of environmentally friendly packaging material.					
36513176	1	35	theme	physicochemical	253:267	arg1	properties					269:278	its physicochemical properties	249:278	its physicochemical properties	249:278	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	0	36	theme	pork	127:130	arg1	preservation					132:143	pork preservation	127:143	pork preservation	127:143	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	0	37	from	film	99:102	arg1	preservation					132:143	pork preservation	127:143	pork preservation	127:143	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	5	38	from	CFU/mL	1041:1046	arg1	samples					1060:1066	the pork samples	1051:1066	the pork samples	1051:1066	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	7	39	theme	CS-EB	1208:1212	arg1	films					1224:1228	the CS-EB composite films	1204:1228	the CS-EB composite films	1204:1228	Overall, the CS-EB composite films can inhibit the growth of bacteria through photodynamic inactivation and has the potential to become a new type of environmentally friendly packaging material.					
36513176	5	40	theme	bacterial	955:963	arg1	growth					965:970	the bacterial growth	951:970	the bacterial growth	951:970	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	7	41	theme	friendly	1361:1368	arg1	material					1380:1387	environmentally friendly packaging material	1345:1387	environmentally friendly packaging material	1345:1387	Overall, the CS-EB composite films can inhibit the growth of bacteria through photodynamic inactivation and has the potential to become a new type of environmentally friendly packaging material.					
36513176	0	42	theme	inactivation	36:47	arg1	properties					49:58	The physiochemical and photodynamic inactivation properties	0:58	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.	0:144	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	3	43	theme	film	723:726	arg1	stability					706:714	the thermal stability	694:714	the thermal stability	694:714	In addition, the elasticity and the thermal stability of the film were enhanced.					
36513176	3	43	theme	film	723:726	arg1	elasticity					679:688	the elasticity	675:688	the elasticity	675:688	In addition, the elasticity and the thermal stability of the film were enhanced.					
36513176	6	44	from	increase	1165:1172	arg1	concentration					1180:1192	EB concentration	1177:1192	EB concentration	1177:1192	Moreover, the antibacterial ability was enhanced with the increase in EB concentration.					
36513176	0	45	from	preservation	132:143	arg1	properties					49:58	The physiochemical and photodynamic inactivation properties	0:58	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.	0:144	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	7	46	theme	composite	1214:1222	arg1	films					1224:1228	the CS-EB composite films	1204:1228	the CS-EB composite films	1204:1228	Overall, the CS-EB composite films can inhibit the growth of bacteria through photodynamic inactivation and has the potential to become a new type of environmentally friendly packaging material.					
36513176	0	47	theme	corn	63:66	arg1	film					99:102	corn starch/erythrosine B composite film	63:102	corn starch/erythrosine B composite film	63:102	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	1	48	theme	photodynamic	284:295	arg1	ability					312:318	photodynamic bacteriostatic ability	284:318	photodynamic bacteriostatic ability	284:318	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	2	49	theme	films	551:555	arg1	content					567:573	moisture content	558:573	moisture content	558:573	The study revealed that the physiochemical properties of CS films: moisture content, water solubility, and water vapor transmission were improved with the addition of EB.					
36513176	2	49	theme	films	551:555	arg1	transmission					610:621	water vapor transmission	598:621	water vapor transmission	598:621	The study revealed that the physiochemical properties of CS films: moisture content, water solubility, and water vapor transmission were improved with the addition of EB.					
36513176	2	49	theme	films	551:555	arg1	properties					534:543	the physiochemical properties	515:543	the physiochemical properties of CS films	515:555	The study revealed that the physiochemical properties of CS films: moisture content, water solubility, and water vapor transmission were improved with the addition of EB.					
36513176	2	49	theme	films	551:555	arg1	solubility					582:591	water solubility	576:591	water solubility	576:591	The study revealed that the physiochemical properties of CS films: moisture content, water solubility, and water vapor transmission were improved with the addition of EB.					
36513176	5	50	theme	illumination	1015:1026	arg1	30 min					1005:1010	30 min	1005:1010	30 min of illumination	1005:1026	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	4	51	theme	CS-EB	771:775	arg1	films					777:781	the CS-EB films	767:781	the CS-EB films	767:781	The results showed that the CS-EB films stimulated a maximum of 26.36 μg/mL hydrogen peroxide and 74.5 μg/g hydroxyl radical under irradiation.					
36513176	4	52	theme	radical	860:866	arg1	maximum					796:802	a maximum	794:802	a maximum of 26.36 μg/mL hydrogen peroxide and 74.5 μg/g hydroxyl radical	794:866	The results showed that the CS-EB films stimulated a maximum of 26.36 μg/mL hydrogen peroxide and 74.5 μg/g hydroxyl radical under irradiation.					
36513176	1	53	theme	bacteriostatic	297:310	arg1	ability					312:318	photodynamic bacteriostatic ability	284:318	photodynamic bacteriostatic ability	284:318	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	2	54	theme	CS	548:549	arg1	films					551:555	CS films	548:555	CS films	548:555	The study revealed that the physiochemical properties of CS films: moisture content, water solubility, and water vapor transmission were improved with the addition of EB.					
36513176	5	55	theme	Log	979:981	arg1	CFU/mL					983:988	4.7 Log CFU/mL	975:988	4.7 Log CFU/mL	975:988	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	1	56	dep	film	240:243	arg1	inoculated					402:411	inoculated	402:411	inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm)	402:488	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	1	56	dep	film	240:243	arg1	both					384:387	both	384:387	both	384:387	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	2	57	theme	EB	658:659	arg1	addition					646:653	the addition	642:653	the addition of EB	642:659	The study revealed that the physiochemical properties of CS films: moisture content, water solubility, and water vapor transmission were improved with the addition of EB.					
36513176	4	58	theme	peroxide	828:835	arg1	maximum					796:802	a maximum	794:802	a maximum of 26.36 μg/mL hydrogen peroxide and 74.5 μg/g hydroxyl radical	794:866	The results showed that the CS-EB films stimulated a maximum of 26.36 μg/mL hydrogen peroxide and 74.5 μg/g hydroxyl radical under irradiation.					
36513176	5	59	theme	same	1078:1081	arg1	condition					1096:1104	the same experimental condition	1074:1104	the same experimental condition	1074:1104	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	1	60	from	photosensitizer	204:218	arg1	properties					269:278	its physicochemical properties	249:278	its physicochemical properties	249:278	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	1	60	from	photosensitizer	204:218	arg1	film					240:243	corn starch (CS) film	223:243	corn starch (CS) film	223:243	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	0	61	from	application	112:122	arg1	preservation					132:143	pork preservation	127:143	pork preservation	127:143	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	7	62	theme	photodynamic	1273:1284	arg1	inactivation					1286:1297	photodynamic inactivation	1273:1297	photodynamic inactivation	1273:1297	Overall, the CS-EB composite films can inhibit the growth of bacteria through photodynamic inactivation and has the potential to become a new type of environmentally friendly packaging material.					
36513176	4	63	theme	hydroxyl	851:858	arg1	radical					860:866	74.5 μg/g hydroxyl radical	841:866	74.5 μg/g hydroxyl radical	841:866	The results showed that the CS-EB films stimulated a maximum of 26.36 μg/mL hydrogen peroxide and 74.5 μg/g hydroxyl radical under irradiation.					
36513176	5	64	theme	EB	938:939	arg1	concentration					921:933	a 5 % concentration	915:933	a 5 % concentration of EB	915:939	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	0	65	theme	B	87:87	arg1	film					99:102	corn starch/erythrosine B composite film	63:102	corn starch/erythrosine B composite film	63:102	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	7	66	theme	bacteria	1256:1263	arg1	growth					1246:1251	the growth	1242:1251	the growth of bacteria	1242:1263	Overall, the CS-EB composite films can inhibit the growth of bacteria through photodynamic inactivation and has the potential to become a new type of environmentally friendly packaging material.					
36513176	5	67	theme	experimental	1083:1094	arg1	condition					1096:1104	the same experimental condition	1074:1104	the same experimental condition	1074:1104	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	1	68	theme	corn	223:226	arg1	film					240:243	corn starch (CS) film	223:243	corn starch (CS) film	223:243	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	0	69	theme	starch/erythrosine	68:85	arg1	film					99:102	corn starch/erythrosine B composite film	63:102	corn starch/erythrosine B composite film	63:102	The physiochemical and photodynamic inactivation properties of corn starch/erythrosine B composite film and its application on pork preservation.					
36513176	1	70	dep	both	384:387	arg1	vitro					392:396	vitro	392:396	vitro	392:396	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	5	71	with	films	904:908	arg1	concentration					921:933	a 5 % concentration	915:933	a 5 % concentration of EB	915:939	The CS composite films with a 5 % concentration of EB inhibited the bacterial growth by 4.7 Log CFU/mL in vitro after 30 min of illumination, and 2.4 Log CFU/mL on the pork samples under the same experimental condition.					
36513176	1	72	dep	diode	465:469	arg1	LED					472:474	LED	472:474	LED	472:474	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36513176	1	73	theme	starch	228:233	arg1	film					240:243	corn starch (CS) film	223:243	corn starch (CS) film	223:243	This study explored the effect of erythrosine B (EB) as a photosensitizer in corn starch (CS) film and its physicochemical properties and photodynamic bacteriostatic ability against Staphylococcus aureus, Escherichia coli, and Salmonella both in vitro and inoculated on pork under the irradiation of D65 light-emitting diode (LED) (400-800 nm).					
36958452	5	0	theme	malondialdehyde	1266:1280	arg1	contents					1230:1237	the contents	1226:1237	the contents of lipid hydroperoxide and malondialdehyde in HIPEs	1226:1289	Moreover, the denser interfacial film based on LZPI-CSPA complexes effectively decreased the contents of lipid hydroperoxide and malondialdehyde in HIPEs, thus improving their oxidation stability.					
36958452	2	1	theme	phase	697:701	arg1	HIPEs					714:718	HIPEs	714:718	HIPEs	714:718	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	2	1	theme	phase	697:701	arg1	emulsions					703:711	high internal phase emulsions	683:711	high internal phase emulsions (HIPEs)	683:719	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	4	2	theme	interconnected	1002:1015	arg1	structure					1025:1033	interconnected network structure	1002:1033	interconnected network structure of LZPI-stabilized HIPEs	1002:1058	Furthermore, the addition of CSPA conjugate promoted the formation of interconnected network structure of LZPI-stabilized HIPEs, thereby endowing them with excellent viscoelasticity and storage stability.					
36958452	2	3	theme	acid	439:442	arg1	conjugate					451:459	chitosan-protocatechuic acid (CSPA) conjugate	415:459	chitosan-protocatechuic acid (CSPA) conjugate	415:459	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	1	4	theme	bioactive	315:323	arg1	components					325:334	bioactive components	315:334	bioactive components	315:334	The delivery vehicles based on protein-polysaccharide-polyphenol are promising methods to encapsulate bioactive components with the aim of improving their solubility and bioavailability.					
36958452	2	5	theme	interfacial	559:569	arg1	architecture					571:582	a composite antioxidant interfacial architecture	535:582	a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs)	535:719	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	2	6	theme	internal	688:695	arg1	HIPEs					714:718	HIPEs	714:718	HIPEs	714:718	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	2	6	theme	internal	688:695	arg1	emulsions					703:711	high internal phase emulsions	683:711	high internal phase emulsions (HIPEs)	683:719	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	2	7	theme	chitosan-protocatechuic	415:437	arg1	conjugate					451:459	chitosan-protocatechuic acid (CSPA) conjugate	415:459	chitosan-protocatechuic acid (CSPA) conjugate	415:459	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	5	8	theme	hydroperoxide	1248:1260	arg1	contents					1230:1237	the contents	1226:1237	the contents of lipid hydroperoxide and malondialdehyde in HIPEs	1226:1289	Moreover, the denser interfacial film based on LZPI-CSPA complexes effectively decreased the contents of lipid hydroperoxide and malondialdehyde in HIPEs, thus improving their oxidation stability.					
36958452	4	9	theme	conjugate	966:974	arg1	addition					949:956	the addition	945:956	the addition of CSPA conjugate	945:974	Furthermore, the addition of CSPA conjugate promoted the formation of interconnected network structure of LZPI-stabilized HIPEs, thereby endowing them with excellent viscoelasticity and storage stability.					
36958452	0	10	theme	hydrophobic	102:112	arg1	nutrients					114:122	hydrophobic nutrients	102:122	hydrophobic nutrients	102:122	High internal phase emulsion gels stabilized by phosphorylated perilla protein isolate for protecting hydrophobic nutrients: Adjusting emulsion performance by incorporating chitosan-protocatechuic acid conjugate.					
36958452	7	11	theme	β-carotene	1547:1556	arg1	bioaccessibility					1527:1542	the bioaccessibility	1523:1542	the bioaccessibility of β-carotene	1523:1556	After in vitro simulated digestion, the bioaccessibility of β-carotene also improved, reaching the highest value in HIPEs containing 1.5 % CSPA conjugate.					
36958452	2	12	theme	high	683:686	arg1	HIPEs					714:718	HIPEs	714:718	HIPEs	714:718	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	2	12	theme	high	683:686	arg1	emulsions					703:711	high internal phase emulsions	683:711	high internal phase emulsions (HIPEs)	683:719	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	6	13	theme	complex-stabilized	1379:1396	arg1	HIPEs					1398:1402	LZPI-CSPA complex-stabilized HIPEs	1369:1402	LZPI-CSPA complex-stabilized HIPEs	1369:1402	The encapsulation of β-carotene by LZPI-CSPA complex-stabilized HIPEs could further enhance its retention rate against different environmental stresses.					
36958452	7	14	theme	1.5 	1620:1623	arg1	%					1624:1624	%	1624:1624	%	1624:1624	After in vitro simulated digestion, the bioaccessibility of β-carotene also improved, reaching the highest value in HIPEs containing 1.5 % CSPA conjugate.					
36958452	8	15	theme	delivery	1702:1709	arg1	vehicles					1711:1718	delivery vehicles	1702:1718	delivery vehicles	1702:1718	These findings will give a reference for the fabrication of delivery vehicles to enhance the stability and bioaccessibility of bioactive components.					
36958452	7	16	theme	%	1624:1624	arg1	conjugate					1631:1639	1.5 % CSPA conjugate	1620:1639	1.5 % CSPA conjugate	1620:1639	After in vitro simulated digestion, the bioaccessibility of β-carotene also improved, reaching the highest value in HIPEs containing 1.5 % CSPA conjugate.					
36958452	6	17	theme	retention	1430:1438	arg1	rate					1440:1443	its retention rate	1426:1443	its retention rate	1426:1443	The encapsulation of β-carotene by LZPI-CSPA complex-stabilized HIPEs could further enhance its retention rate against different environmental stresses.					
36958452	7	18	theme	in	1493:1494	arg1	digestion					1512:1520	in vitro simulated digestion	1493:1520	in vitro simulated digestion	1493:1520	After in vitro simulated digestion, the bioaccessibility of β-carotene also improved, reaching the highest value in HIPEs containing 1.5 % CSPA conjugate.					
36958452	2	19	theme	lipid	593:597	arg1	oxidation					599:607	lipid oxidation	593:607	lipid oxidation	593:607	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	5	20	theme	lipid	1242:1246	arg1	hydroperoxide					1248:1260	lipid hydroperoxide	1242:1260	lipid hydroperoxide	1242:1260	Moreover, the denser interfacial film based on LZPI-CSPA complexes effectively decreased the contents of lipid hydroperoxide and malondialdehyde in HIPEs, thus improving their oxidation stability.					
36958452	8	21	theme	components	1779:1788	arg1	stability					1735:1743	stability	1735:1743	stability	1735:1743	These findings will give a reference for the fabrication of delivery vehicles to enhance the stability and bioaccessibility of bioactive components.					
36958452	8	21	theme	components	1779:1788	arg1	bioaccessibility					1749:1764	bioaccessibility	1749:1764	bioaccessibility	1749:1764	These findings will give a reference for the fabrication of delivery vehicles to enhance the stability and bioaccessibility of bioactive components.					
36958452	0	22	theme	internal	5:12	arg1	gels					29:32	High internal phase emulsion gels	0:32	High internal phase emulsion gels stabilized by phosphorylated perilla protein	0:77	High internal phase emulsion gels stabilized by phosphorylated perilla protein isolate for protecting hydrophobic nutrients: Adjusting emulsion performance by incorporating chitosan-protocatechuic acid conjugate.					
36958452	3	23	theme	electrostatic	792:804	arg1	interaction					806:816	electrostatic interaction	792:816	electrostatic interaction	792:816	Compared to LZPI, the LZPI-CSPA complexes formed by hydrogen bond and electrostatic interaction showed improved wettability and reduced interfacial tension, which facilitated their adsorption at the interface.					
36958452	1	24	theme	delivery	217:224	arg1	vehicles					226:233	The delivery vehicles	213:233	The delivery vehicles based on protein-polysaccharide-polyphenol	213:276	The delivery vehicles based on protein-polysaccharide-polyphenol are promising methods to encapsulate bioactive components with the aim of improving their solubility and bioavailability.					
36958452	1	24	theme	delivery	217:224	arg1	methods					292:298	promising methods	282:298	promising methods	282:298	The delivery vehicles based on protein-polysaccharide-polyphenol are promising methods to encapsulate bioactive components with the aim of improving their solubility and bioavailability.					
36958452	0	25	theme	High	0:3	arg1	gels					29:32	High internal phase emulsion gels	0:32	High internal phase emulsion gels stabilized by phosphorylated perilla protein	0:77	High internal phase emulsion gels stabilized by phosphorylated perilla protein isolate for protecting hydrophobic nutrients: Adjusting emulsion performance by incorporating chitosan-protocatechuic acid conjugate.					
36958452	5	26	theme	oxidation	1313:1321	arg1	stability					1323:1331	their oxidation stability	1307:1331	their oxidation stability	1307:1331	Moreover, the denser interfacial film based on LZPI-CSPA complexes effectively decreased the contents of lipid hydroperoxide and malondialdehyde in HIPEs, thus improving their oxidation stability.					
36958452	4	27	theme	CSPA	961:964	arg1	conjugate					966:974	CSPA conjugate	961:974	CSPA conjugate	961:974	Furthermore, the addition of CSPA conjugate promoted the formation of interconnected network structure of LZPI-stabilized HIPEs, thereby endowing them with excellent viscoelasticity and storage stability.					
36958452	0	28	theme	emulsion	20:27	arg1	gels					29:32	High internal phase emulsion gels	0:32	High internal phase emulsion gels stabilized by phosphorylated perilla protein	0:77	High internal phase emulsion gels stabilized by phosphorylated perilla protein isolate for protecting hydrophobic nutrients: Adjusting emulsion performance by incorporating chitosan-protocatechuic acid conjugate.					
36958452	2	29	theme	digestion	640:648	arg1	profiles					650:657	digestion profiles	640:657	digestion profiles	640:657	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	6	30	theme	LZPI-CSPA	1369:1377	arg1	HIPEs					1398:1402	LZPI-CSPA complex-stabilized HIPEs	1369:1402	LZPI-CSPA complex-stabilized HIPEs	1369:1402	The encapsulation of β-carotene by LZPI-CSPA complex-stabilized HIPEs could further enhance its retention rate against different environmental stresses.					
36958452	0	31	theme	phase	14:18	arg1	gels					29:32	High internal phase emulsion gels	0:32	High internal phase emulsion gels stabilized by phosphorylated perilla protein	0:77	High internal phase emulsion gels stabilized by phosphorylated perilla protein isolate for protecting hydrophobic nutrients: Adjusting emulsion performance by incorporating chitosan-protocatechuic acid conjugate.					
36958452	4	32	theme	structure	1025:1033	arg1	formation					989:997	the formation	985:997	the formation of interconnected network structure of LZPI-stabilized HIPEs	985:1058	Furthermore, the addition of CSPA conjugate promoted the formation of interconnected network structure of LZPI-stabilized HIPEs, thereby endowing them with excellent viscoelasticity and storage stability.					
36958452	4	33	theme	LZPI-stabilized	1038:1052	arg1	HIPEs					1054:1058	LZPI-stabilized HIPEs	1038:1058	LZPI-stabilized HIPEs	1038:1058	Furthermore, the addition of CSPA conjugate promoted the formation of interconnected network structure of LZPI-stabilized HIPEs, thereby endowing them with excellent viscoelasticity and storage stability.					
36958452	7	34	theme	highest	1586:1592	arg1	value					1594:1598	the highest value	1582:1598	the highest value in HIPEs containing 1.5 % CSPA conjugate	1582:1639	After in vitro simulated digestion, the bioaccessibility of β-carotene also improved, reaching the highest value in HIPEs containing 1.5 % CSPA conjugate.					
36958452	2	35	theme	protein	500:506	arg1	LZPI					517:520	LZPI	517:520	LZPI	517:520	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	2	35	theme	protein	500:506	arg1	isolate					508:514	phosphorylated perilla protein isolate	477:514	phosphorylated perilla protein isolate (LZPI)	477:521	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	2	36	theme	antioxidant	547:557	arg1	architecture					571:582	a composite antioxidant interfacial architecture	535:582	a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs)	535:719	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	8	37	theme	bioactive	1769:1777	arg1	components					1779:1788	bioactive components	1769:1788	bioactive components	1769:1788	These findings will give a reference for the fabrication of delivery vehicles to enhance the stability and bioaccessibility of bioactive components.					
36958452	4	38	theme	HIPEs	1054:1058	arg1	structure					1025:1033	interconnected network structure	1002:1033	interconnected network structure of LZPI-stabilized HIPEs	1002:1058	Furthermore, the addition of CSPA conjugate promoted the formation of interconnected network structure of LZPI-stabilized HIPEs, thereby endowing them with excellent viscoelasticity and storage stability.					
36958452	6	39	theme	environmental	1463:1475	arg1	stresses					1477:1484	different environmental stresses	1453:1484	different environmental stresses	1453:1484	The encapsulation of β-carotene by LZPI-CSPA complex-stabilized HIPEs could further enhance its retention rate against different environmental stresses.					
36958452	7	40	theme	simulated	1502:1510	arg1	digestion					1512:1520	in vitro simulated digestion	1493:1520	in vitro simulated digestion	1493:1520	After in vitro simulated digestion, the bioaccessibility of β-carotene also improved, reaching the highest value in HIPEs containing 1.5 % CSPA conjugate.					
36958452	2	41	theme	perilla	492:498	arg1	LZPI					517:520	LZPI	517:520	LZPI	517:520	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	2	41	theme	perilla	492:498	arg1	isolate					508:514	phosphorylated perilla protein isolate	477:514	phosphorylated perilla protein isolate (LZPI)	477:521	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	2	42	theme	composite	537:545	arg1	architecture					571:582	a composite antioxidant interfacial architecture	535:582	a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs)	535:719	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	6	43	theme	different	1453:1461	arg1	stresses					1477:1484	different environmental stresses	1453:1484	different environmental stresses	1453:1484	The encapsulation of β-carotene by LZPI-CSPA complex-stabilized HIPEs could further enhance its retention rate against different environmental stresses.					
36958452	4	44	theme	storage	1118:1124	arg1	stability					1126:1134	storage stability	1118:1134	storage stability	1118:1134	Furthermore, the addition of CSPA conjugate promoted the formation of interconnected network structure of LZPI-stabilized HIPEs, thereby endowing them with excellent viscoelasticity and storage stability.					
36958452	5	45	from	contents	1230:1237	arg1	HIPEs					1285:1289	HIPEs	1285:1289	HIPEs	1285:1289	Moreover, the denser interfacial film based on LZPI-CSPA complexes effectively decreased the contents of lipid hydroperoxide and malondialdehyde in HIPEs, thus improving their oxidation stability.					
36958452	2	46	theme	phosphorylated	477:490	arg1	LZPI					517:520	LZPI	517:520	LZPI	517:520	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	2	46	theme	phosphorylated	477:490	arg1	isolate					508:514	phosphorylated perilla protein isolate	477:514	phosphorylated perilla protein isolate (LZPI)	477:521	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	0	47	theme	phosphorylated	48:61	arg1	protein					71:77	phosphorylated perilla protein	48:77	phosphorylated perilla protein	48:77	High internal phase emulsion gels stabilized by phosphorylated perilla protein isolate for protecting hydrophobic nutrients: Adjusting emulsion performance by incorporating chitosan-protocatechuic acid conjugate.					
36958452	2	48	dep	stability	626:634	arg1	the					622:624	the	622:624	the	622:624	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	0	49	theme	chitosan-protocatechuic	173:195	arg1	conjugate					202:210	chitosan-protocatechuic acid conjugate	173:210	chitosan-protocatechuic acid conjugate	173:210	High internal phase emulsion gels stabilized by phosphorylated perilla protein isolate for protecting hydrophobic nutrients: Adjusting emulsion performance by incorporating chitosan-protocatechuic acid conjugate.					
36958452	5	50	theme	denser	1151:1156	arg1	film					1170:1173	the denser interfacial film	1147:1173	the denser interfacial film based on LZPI-CSPA complexes	1147:1202	Moreover, the denser interfacial film based on LZPI-CSPA complexes effectively decreased the contents of lipid hydroperoxide and malondialdehyde in HIPEs, thus improving their oxidation stability.					
36958452	7	51	theme	CSPA	1626:1629	arg1	conjugate					1631:1639	1.5 % CSPA conjugate	1620:1639	1.5 % CSPA conjugate	1620:1639	After in vitro simulated digestion, the bioaccessibility of β-carotene also improved, reaching the highest value in HIPEs containing 1.5 % CSPA conjugate.					
36958452	0	52	theme	emulsion	135:142	arg1	performance					144:154	emulsion performance	135:154	emulsion performance	135:154	High internal phase emulsion gels stabilized by phosphorylated perilla protein isolate for protecting hydrophobic nutrients: Adjusting emulsion performance by incorporating chitosan-protocatechuic acid conjugate.					
36958452	3	53	theme	interfacial	858:868	arg1	tension					870:876	interfacial tension	858:876	interfacial tension	858:876	Compared to LZPI, the LZPI-CSPA complexes formed by hydrogen bond and electrostatic interaction showed improved wettability and reduced interfacial tension, which facilitated their adsorption at the interface.					
36958452	2	54	theme	β-carotene	662:671	arg1	stability					626:634	stability	626:634	stability	626:634	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	2	54	theme	β-carotene	662:671	arg1	profiles					650:657	digestion profiles	640:657	digestion profiles	640:657	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	3	55	theme	LZPI-CSPA	744:752	arg1	complexes					754:762	the LZPI-CSPA complexes	740:762	the LZPI-CSPA complexes formed by hydrogen bond and electrostatic interaction	740:816	Compared to LZPI, the LZPI-CSPA complexes formed by hydrogen bond and electrostatic interaction showed improved wettability and reduced interfacial tension, which facilitated their adsorption at the interface.					
36958452	5	56	theme	interfacial	1158:1168	arg1	film					1170:1173	the denser interfacial film	1147:1173	the denser interfacial film based on LZPI-CSPA complexes	1147:1202	Moreover, the denser interfacial film based on LZPI-CSPA complexes effectively decreased the contents of lipid hydroperoxide and malondialdehyde in HIPEs, thus improving their oxidation stability.					
36958452	7	57	contain	containing	1609:1618	arg2	conjugate					1631:1639	1.5 % CSPA conjugate	1620:1639	1.5 % CSPA conjugate	1620:1639	After in vitro simulated digestion, the bioaccessibility of β-carotene also improved, reaching the highest value in HIPEs containing 1.5 % CSPA conjugate.					
36958452	7	57	contain	containing	1609:1618	arg1	HIPEs					1603:1607	HIPEs	1603:1607	HIPEs containing 1.5 % CSPA conjugate	1603:1639	After in vitro simulated digestion, the bioaccessibility of β-carotene also improved, reaching the highest value in HIPEs containing 1.5 % CSPA conjugate.					
36958452	3	58	dep	showed	818:823	arg1	improved					825:832	improved	825:832	improved wettability	825:844	Compared to LZPI, the LZPI-CSPA complexes formed by hydrogen bond and electrostatic interaction showed improved wettability and reduced interfacial tension, which facilitated their adsorption at the interface.					
36958452	3	58	dep	showed	818:823	arg1	reduced					850:856	reduced	850:856	reduced interfacial tension, which facilitated their adsorption at the interface	850:929	Compared to LZPI, the LZPI-CSPA complexes formed by hydrogen bond and electrostatic interaction showed improved wettability and reduced interfacial tension, which facilitated their adsorption at the interface.					
36958452	4	59	theme	network	1017:1023	arg1	structure					1025:1033	interconnected network structure	1002:1033	interconnected network structure of LZPI-stabilized HIPEs	1002:1058	Furthermore, the addition of CSPA conjugate promoted the formation of interconnected network structure of LZPI-stabilized HIPEs, thereby endowing them with excellent viscoelasticity and storage stability.					
36958452	8	60	dep	stability	1735:1743	arg1	the					1731:1733	the	1731:1733	the	1731:1733	These findings will give a reference for the fabrication of delivery vehicles to enhance the stability and bioaccessibility of bioactive components.					
36958452	1	61	theme	promising	282:290	arg1	vehicles					226:233	The delivery vehicles	213:233	The delivery vehicles based on protein-polysaccharide-polyphenol	213:276	The delivery vehicles based on protein-polysaccharide-polyphenol are promising methods to encapsulate bioactive components with the aim of improving their solubility and bioavailability.					
36958452	1	61	theme	promising	282:290	arg1	methods					292:298	promising methods	282:298	promising methods	282:298	The delivery vehicles based on protein-polysaccharide-polyphenol are promising methods to encapsulate bioactive components with the aim of improving their solubility and bioavailability.					
36958452	0	62	theme	acid	197:200	arg1	conjugate					202:210	chitosan-protocatechuic acid conjugate	173:210	chitosan-protocatechuic acid conjugate	173:210	High internal phase emulsion gels stabilized by phosphorylated perilla protein isolate for protecting hydrophobic nutrients: Adjusting emulsion performance by incorporating chitosan-protocatechuic acid conjugate.					
36958452	6	63	theme	β-carotene	1355:1364	arg1	encapsulation					1338:1350	The encapsulation	1334:1350	The encapsulation of β-carotene by LZPI-CSPA complex-stabilized HIPEs	1334:1402	The encapsulation of β-carotene by LZPI-CSPA complex-stabilized HIPEs could further enhance its retention rate against different environmental stresses.					
36958452	7	64	from	value	1594:1598	arg1	HIPEs					1603:1607	HIPEs	1603:1607	HIPEs containing 1.5 % CSPA conjugate	1603:1639	After in vitro simulated digestion, the bioaccessibility of β-carotene also improved, reaching the highest value in HIPEs containing 1.5 % CSPA conjugate.					
36958452	4	65	theme	excellent	1088:1096	arg1	viscoelasticity					1098:1112	excellent viscoelasticity	1088:1112	excellent viscoelasticity	1088:1112	Furthermore, the addition of CSPA conjugate promoted the formation of interconnected network structure of LZPI-stabilized HIPEs, thereby endowing them with excellent viscoelasticity and storage stability.					
36958452	5	66	theme	LZPI-CSPA	1184:1192	arg1	complexes					1194:1202	LZPI-CSPA complexes	1184:1202	LZPI-CSPA complexes	1184:1202	Moreover, the denser interfacial film based on LZPI-CSPA complexes effectively decreased the contents of lipid hydroperoxide and malondialdehyde in HIPEs, thus improving their oxidation stability.					
36958452	8	67	theme	vehicles	1711:1718	arg1	fabrication					1687:1697	the fabrication	1683:1697	the fabrication of delivery vehicles to enhance the stability and bioaccessibility of bioactive components	1683:1788	These findings will give a reference for the fabrication of delivery vehicles to enhance the stability and bioaccessibility of bioactive components.					
36958452	2	68	theme	CSPA	445:448	arg1	conjugate					451:459	chitosan-protocatechuic acid (CSPA) conjugate	415:459	chitosan-protocatechuic acid (CSPA) conjugate	415:459	In this study, chitosan-protocatechuic acid (CSPA) conjugate interacted with phosphorylated perilla protein isolate (LZPI) to engineer a composite antioxidant interfacial architecture to delay lipid oxidation and regulate the stability and digestion profiles of β-carotene loaded in high internal phase emulsions (HIPEs).					
36958452	0	69	theme	perilla	63:69	arg1	protein					71:77	phosphorylated perilla protein	48:77	phosphorylated perilla protein	48:77	High internal phase emulsion gels stabilized by phosphorylated perilla protein isolate for protecting hydrophobic nutrients: Adjusting emulsion performance by incorporating chitosan-protocatechuic acid conjugate.					
36958452	3	70	theme	hydrogen	774:781	arg1	bond					783:786	hydrogen bond	774:786	hydrogen bond	774:786	Compared to LZPI, the LZPI-CSPA complexes formed by hydrogen bond and electrostatic interaction showed improved wettability and reduced interfacial tension, which facilitated their adsorption at the interface.					
36958452	7	71	dep	in	1493:1494	arg1	vitro					1496:1500	vitro	1496:1500	vitro	1496:1500	After in vitro simulated digestion, the bioaccessibility of β-carotene also improved, reaching the highest value in HIPEs containing 1.5 % CSPA conjugate.					
35868782	7	0	theme	CT1	1081:1083	arg1	fibers					1106:1111	CT1 conjugated-cellulose fibers	1081:1111	CT1 conjugated-cellulose fibers	1081:1111	We believe that the application of CT1 conjugated-cellulose fibers is not limited to the high-performance PPE, and also can be extended to various types of protective equipment for food and medicine safety.					
35868782	7	1	dep	food	1227:1230	arg1	safety					1245:1250	safety	1245:1250	safety	1245:1250	We believe that the application of CT1 conjugated-cellulose fibers is not limited to the high-performance PPE, and also can be extended to various types of protective equipment for food and medicine safety.					
35868782	5	2	theme	%	758:758	arg1	%					765:765	7.19 %-7.42 %	753:765	7.19 %-7.42 %	753:765	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	6	3	theme	%	915:915	arg1	coli					889:892	Escherichia coli	877:892	Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %)	877:925	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	6	3	theme	%	915:915	arg1	%					924:924	GIR: 61.5 % ± 1.28 %	905:924	GIR: 61.5 % ± 1.28 %	905:924	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	3	4	theme	effective	416:424	arg1	CT1-4					476:480	CT1-4	476:480	CT1-4	476:480	Here, we grafted highly effective antibacterial copper-thiosemicarbazone complexes (CT1-4) on cellulose fibers via covalent linkages.					
35868782	3	4	theme	effective	416:424	arg1	complexes					465:473	highly effective antibacterial copper-thiosemicarbazone complexes	409:473	highly effective antibacterial copper-thiosemicarbazone complexes (CT1-4)	409:481	Here, we grafted highly effective antibacterial copper-thiosemicarbazone complexes (CT1-4) on cellulose fibers via covalent linkages.					
35868782	6	5	dep	subtilis	999:1006	arg1	subtilis					1012:1019	B. subtilis	1009:1019	B. subtilis	1009:1019	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	3	6	theme	antibacterial	426:438	arg1	CT1-4					476:480	CT1-4	476:480	CT1-4	476:480	Here, we grafted highly effective antibacterial copper-thiosemicarbazone complexes (CT1-4) on cellulose fibers via covalent linkages.					
35868782	3	6	theme	antibacterial	426:438	arg1	complexes					465:473	highly effective antibacterial copper-thiosemicarbazone complexes	409:473	highly effective antibacterial copper-thiosemicarbazone complexes (CT1-4)	409:481	Here, we grafted highly effective antibacterial copper-thiosemicarbazone complexes (CT1-4) on cellulose fibers via covalent linkages.					
35868782	6	7	theme	excellent	841:849	arg1	inhibition					858:867	the excellent growth inhibition	837:867	the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %)	837:1043	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	2	8	theme	antibacterial	333:345	arg1	fibers					347:352	biodegradable and antibacterial fibers	315:352	biodegradable and antibacterial fibers	315:352	Our work focused on developing biodegradable and antibacterial fibers as promising bioprotective materials.					
35868782	2	8	theme	antibacterial	333:345	arg1	materials					381:389	promising bioprotective materials	357:389	promising bioprotective materials	357:389	Our work focused on developing biodegradable and antibacterial fibers as promising bioprotective materials.					
35868782	4	9	theme	chemical	573:580	arg1	composition					582:592	composition	582:592	composition	582:592	Multiple methods were used to characterize the chemical composition or morphology of CT1-4 conjugated-fibers.					
35868782	0	10	theme	Biodegradable	64:76	arg1	materials					78:86	Biodegradable materials	64:86	fibers: Biodegradable materials with antibacterial capacity	56:114	Copper-thiosemicarbazone complexes conjugated-cellulose fibers: Biodegradable materials with antibacterial capacity.					
35868782	5	11	theme	mechanical	671:680	arg1	properties					682:691	the mechanical properties	667:691	the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %)	667:766	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	5	11	theme	mechanical	671:680	arg1	strength					703:710	Breaking strength	694:710	Breaking strength	694:710	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	1	12	theme	Personal	117:124	arg1	equipment					137:145	Personal protective equipment	117:145	Personal protective equipment (PPE)	117:151	Personal protective equipment (PPE) is vital in battling bacteria crisis, but conventional PPE materials lack antimicrobial activities and environmental friendliness.					
35868782	1	12	theme	Personal	117:124	arg1	PPE					148:150	PPE	148:150	PPE	148:150	Personal protective equipment (PPE) is vital in battling bacteria crisis, but conventional PPE materials lack antimicrobial activities and environmental friendliness.					
35868782	6	13	dep	coli	889:892	arg1	GIR					1023:1025	GIR	1023:1025	GIR: 87.6 % ± 1.44 %	1023:1042	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	6	13	dep	coli	889:892	arg1	coli					898:901	E. coli	895:901	E. coli	895:901	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	1	14	theme	conventional	195:206	arg1	materials					212:220	conventional PPE materials	195:220	conventional PPE materials	195:220	Personal protective equipment (PPE) is vital in battling bacteria crisis, but conventional PPE materials lack antimicrobial activities and environmental friendliness.					
35868782	7	15	theme	high-performance	1135:1150	arg1	PPE					1152:1154	the high-performance PPE	1131:1154	the high-performance PPE	1131:1154	We believe that the application of CT1 conjugated-cellulose fibers is not limited to the high-performance PPE, and also can be extended to various types of protective equipment for food and medicine safety.					
35868782	1	16	theme	protective	126:135	arg1	equipment					137:145	Personal protective equipment	117:145	Personal protective equipment (PPE)	117:151	Personal protective equipment (PPE) is vital in battling bacteria crisis, but conventional PPE materials lack antimicrobial activities and environmental friendliness.					
35868782	1	16	theme	protective	126:135	arg1	PPE					148:150	PPE	148:150	PPE	148:150	Personal protective equipment (PPE) is vital in battling bacteria crisis, but conventional PPE materials lack antimicrobial activities and environmental friendliness.					
35868782	1	17	theme	PPE	208:210	arg1	materials					212:220	conventional PPE materials	195:220	conventional PPE materials	195:220	Personal protective equipment (PPE) is vital in battling bacteria crisis, but conventional PPE materials lack antimicrobial activities and environmental friendliness.					
35868782	0	18	theme	Copper-thiosemicarbazone	0:23	arg1	complexes					25:33	Copper-thiosemicarbazone complexes	0:33	Copper-thiosemicarbazone complexes	0:33	Copper-thiosemicarbazone complexes conjugated-cellulose fibers: Biodegradable materials with antibacterial capacity.					
35868782	2	19	theme	bioprotective	367:379	arg1	fibers					347:352	biodegradable and antibacterial fibers	315:352	biodegradable and antibacterial fibers	315:352	Our work focused on developing biodegradable and antibacterial fibers as promising bioprotective materials.					
35868782	2	19	theme	bioprotective	367:379	arg1	materials					381:389	promising bioprotective materials	357:389	promising bioprotective materials	357:389	Our work focused on developing biodegradable and antibacterial fibers as promising bioprotective materials.					
35868782	3	20	theme	covalent	507:514	arg1	linkages					516:523	covalent linkages	507:523	covalent linkages	507:523	Here, we grafted highly effective antibacterial copper-thiosemicarbazone complexes (CT1-4) on cellulose fibers via covalent linkages.					
35868782	3	21	theme	copper-thiosemicarbazone	440:463	arg1	CT1-4					476:480	CT1-4	476:480	CT1-4	476:480	Here, we grafted highly effective antibacterial copper-thiosemicarbazone complexes (CT1-4) on cellulose fibers via covalent linkages.					
35868782	3	21	theme	copper-thiosemicarbazone	440:463	arg1	complexes					465:473	highly effective antibacterial copper-thiosemicarbazone complexes	409:473	highly effective antibacterial copper-thiosemicarbazone complexes (CT1-4)	409:481	Here, we grafted highly effective antibacterial copper-thiosemicarbazone complexes (CT1-4) on cellulose fibers via covalent linkages.					
35868782	5	22	theme	Breaking	694:701	arg1	properties					682:691	the mechanical properties	667:691	the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %)	667:766	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	5	22	theme	Breaking	694:701	arg1	strength					703:710	Breaking strength	694:710	Breaking strength	694:710	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	2	23	theme	promising	357:365	arg1	fibers					347:352	biodegradable and antibacterial fibers	315:352	biodegradable and antibacterial fibers	315:352	Our work focused on developing biodegradable and antibacterial fibers as promising bioprotective materials.					
35868782	2	23	theme	promising	357:365	arg1	materials					381:389	promising bioprotective materials	357:389	promising bioprotective materials	357:389	Our work focused on developing biodegradable and antibacterial fibers as promising bioprotective materials.					
35868782	6	24	theme	%	1033:1033	arg1	%					1042:1042	87.6 % ± 1.44 %	1028:1042	GIR: 87.6 % ± 1.44 %	1023:1042	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	6	25	theme	cellulose	815:823	arg1	fibers					825:830	cellulose fibers	815:830	cellulose fibers	815:830	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	6	26	dep	GIR	1023:1025	arg1	%					1042:1042	87.6 % ± 1.44 %	1028:1042	GIR: 87.6 % ± 1.44 %	1023:1042	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	5	27	theme	fibers	793:798	arg1	stability					780:788	thermal stability	772:788	thermal stability of fibers	772:798	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	5	27	theme	fibers	793:798	arg1	properties					682:691	the mechanical properties	667:691	the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %)	667:766	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	5	27	theme	fibers	793:798	arg1	strength					703:710	Breaking strength	694:710	Breaking strength	694:710	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	1	28	theme	antimicrobial	227:239	arg1	activities					241:250	antimicrobial activities	227:250	antimicrobial activities	227:250	Personal protective equipment (PPE) is vital in battling bacteria crisis, but conventional PPE materials lack antimicrobial activities and environmental friendliness.					
35868782	3	29	theme	cellulose	486:494	arg1	fibers					496:501	cellulose fibers	486:501	cellulose fibers	486:501	Here, we grafted highly effective antibacterial copper-thiosemicarbazone complexes (CT1-4) on cellulose fibers via covalent linkages.					
35868782	6	30	theme	 ± 1.93 	974:981	arg1	%					982:982	85.7 % ± 1.93 %	968:982	GIR: 85.7 % ± 1.93 %	963:982	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	4	31	theme	Multiple	526:533	arg1	methods					535:541	Multiple methods	526:541	Multiple methods	526:541	Multiple methods were used to characterize the chemical composition or morphology of CT1-4 conjugated-fibers.					
35868782	6	32	theme	 ± 1.44 	1034:1041	arg1	%					1042:1042	87.6 % ± 1.44 %	1028:1042	GIR: 87.6 % ± 1.44 %	1023:1042	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	7	33	theme	fibers	1106:1111	arg1	limited					1120:1126	limited	1120:1126	limited	1120:1126	We believe that the application of CT1 conjugated-cellulose fibers is not limited to the high-performance PPE, and also can be extended to various types of protective equipment for food and medicine safety.					
35868782	7	33	theme	fibers	1106:1111	arg1	application					1066:1076	the application	1062:1076	the application of CT1 conjugated-cellulose fibers	1062:1111	We believe that the application of CT1 conjugated-cellulose fibers is not limited to the high-performance PPE, and also can be extended to various types of protective equipment for food and medicine safety.					
35868782	4	34	theme	conjugated-fibers	617:633	arg1	morphology					597:606	morphology	597:606	morphology	597:606	Multiple methods were used to characterize the chemical composition or morphology of CT1-4 conjugated-fibers.					
35868782	4	34	theme	conjugated-fibers	617:633	arg1	composition					582:592	composition	582:592	composition	582:592	Multiple methods were used to characterize the chemical composition or morphology of CT1-4 conjugated-fibers.					
35868782	6	35	theme	 ± 1.28 	916:923	arg1	coli					889:892	Escherichia coli	877:892	Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %)	877:925	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	6	35	theme	 ± 1.28 	916:923	arg1	%					924:924	GIR: 61.5 % ± 1.28 %	905:924	GIR: 61.5 % ± 1.28 %	905:924	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	6	36	theme	growth	851:856	arg1	inhibition					858:867	the excellent growth inhibition	837:867	the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %)	837:1043	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	7	37	theme	conjugated-cellulose	1085:1104	arg1	fibers					1106:1111	CT1 conjugated-cellulose fibers	1081:1111	CT1 conjugated-cellulose fibers	1081:1111	We believe that the application of CT1 conjugated-cellulose fibers is not limited to the high-performance PPE, and also can be extended to various types of protective equipment for food and medicine safety.					
35868782	4	38	theme	CT1-4	611:615	arg1	conjugated-fibers					617:633	CT1-4 conjugated-fibers	611:633	CT1-4 conjugated-fibers	611:633	Multiple methods were used to characterize the chemical composition or morphology of CT1-4 conjugated-fibers.					
35868782	6	39	dep	GIR	963:965	arg1	%					982:982	85.7 % ± 1.93 %	968:982	GIR: 85.7 % ± 1.93 %	963:982	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	0	40	theme	antibacterial	93:105	arg1	capacity					107:114	antibacterial capacity	93:114	antibacterial capacity	93:114	Copper-thiosemicarbazone complexes conjugated-cellulose fibers: Biodegradable materials with antibacterial capacity.					
35868782	5	41	theme	CT1-4	651:655	arg1	Conjugation					636:646	Conjugation	636:646	Conjugation of CT1-4	636:655	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	6	42	theme	%	973:973	arg1	%					982:982	85.7 % ± 1.93 %	968:982	GIR: 85.7 % ± 1.93 %	963:982	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	6	43	theme	61.5 	910:914	arg1	coli					889:892	Escherichia coli	877:892	Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %)	877:925	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	6	43	theme	61.5 	910:914	arg1	%					924:924	GIR: 61.5 % ± 1.28 %	905:924	GIR: 61.5 % ± 1.28 %	905:924	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	5	44	theme	Breaking	732:739	arg1	elongation					741:750	Breaking elongation	732:750	Breaking elongation	732:750	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	4	45	dep	composition	582:592	arg1	the					569:571	the	569:571	the	569:571	Multiple methods were used to characterize the chemical composition or morphology of CT1-4 conjugated-fibers.					
35868782	5	46	theme	thermal	772:778	arg1	stability					780:788	thermal stability	772:788	thermal stability of fibers	772:798	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	0	47	dep	fibers	56:61	arg1	materials					78:86	Biodegradable materials	64:86	fibers: Biodegradable materials with antibacterial capacity	56:114	Copper-thiosemicarbazone complexes conjugated-cellulose fibers: Biodegradable materials with antibacterial capacity.					
35868782	1	48	theme	environmental	256:268	arg1	friendliness					270:281	environmental friendliness	256:281	environmental friendliness	256:281	Personal protective equipment (PPE) is vital in battling bacteria crisis, but conventional PPE materials lack antimicrobial activities and environmental friendliness.					
35868782	2	49	theme	biodegradable	315:327	arg1	fibers					347:352	biodegradable and antibacterial fibers	315:352	biodegradable and antibacterial fibers	315:352	Our work focused on developing biodegradable and antibacterial fibers as promising bioprotective materials.					
35868782	2	49	theme	biodegradable	315:327	arg1	materials					381:389	promising bioprotective materials	357:389	promising bioprotective materials	357:389	Our work focused on developing biodegradable and antibacterial fibers as promising bioprotective materials.					
35868782	6	50	theme	GIR	905:907	arg1	coli					889:892	Escherichia coli	877:892	Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %)	877:925	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	6	50	theme	GIR	905:907	arg1	%					924:924	GIR: 61.5 % ± 1.28 %	905:924	GIR: 61.5 % ± 1.28 %	905:924	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	7	51	theme	equipment	1213:1221	arg1	types					1193:1197	various types	1185:1197	various types of protective equipment for food and medicine safety	1185:1250	We believe that the application of CT1 conjugated-cellulose fibers is not limited to the high-performance PPE, and also can be extended to various types of protective equipment for food and medicine safety.					
35868782	5	52	dep	strength	703:710	arg1	elongation					741:750	Breaking elongation	732:750	Breaking elongation	732:750	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	5	52	dep	strength	703:710	arg1	cN/dtex					723:729	2.35-2.45 cN/dtex	713:729	2.35-2.45 cN/dtex	713:729	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	5	52	dep	strength	703:710	arg1	%					765:765	7.19 %-7.42 %	753:765	7.19 %-7.42 %	753:765	Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers.					
35868782	0	53	with	materials	78:86	arg1	capacity					107:114	antibacterial capacity	93:114	antibacterial capacity	93:114	Copper-thiosemicarbazone complexes conjugated-cellulose fibers: Biodegradable materials with antibacterial capacity.					
35868782	7	54	theme	protective	1202:1211	arg1	equipment					1213:1221	protective equipment	1202:1221	protective equipment for food and medicine safety	1202:1250	We believe that the application of CT1 conjugated-cellulose fibers is not limited to the high-performance PPE, and also can be extended to various types of protective equipment for food and medicine safety.					
35868782	7	55	theme	various	1185:1191	arg1	types					1193:1197	various types	1185:1197	various types of protective equipment for food and medicine safety	1185:1250	We believe that the application of CT1 conjugated-cellulose fibers is not limited to the high-performance PPE, and also can be extended to various types of protective equipment for food and medicine safety.					
35868782	4	56	used	used	548:551	arg2	methods					535:541	Multiple methods	526:541	Multiple methods	526:541	Multiple methods were used to characterize the chemical composition or morphology of CT1-4 conjugated-fibers.					
35868782	1	57	theme	bacteria	174:181	arg1	crisis					183:188	bacteria crisis	174:188	bacteria crisis	174:188	Personal protective equipment (PPE) is vital in battling bacteria crisis, but conventional PPE materials lack antimicrobial activities and environmental friendliness.					
35868782	6	58	dep	aureus	943:948	arg1	aureus					954:959	S. aureus	951:959	S. aureus	951:959	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
35868782	6	58	dep	aureus	943:948	arg1	GIR					963:965	GIR	963:965	GIR: 85.7 % ± 1.93 %	963:982	CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %).					
37352956	0	0	theme	applications	75:86	arg1	formulation					6:16	A new formulation	0:16	A new formulation of Ni/Zn bi-metallic nanocomposite	0:51	A new formulation of Ni/Zn bi-metallic nanocomposite and evaluation of its applications for pollution removal, photocatalytic, electrochemical sensing, and anti-breast cancer.					
37352956	0	0	theme	applications	75:86	arg1	evaluation					57:66	evaluation	57:66	evaluation of its applications for pollution removal, photocatalytic, electrochemical sensing, and anti-breast cancer	57:173	A new formulation of Ni/Zn bi-metallic nanocomposite and evaluation of its applications for pollution removal, photocatalytic, electrochemical sensing, and anti-breast cancer.					
37352956	11	1	theme	breast	1489:1494	arg1	lines					1516:1520	T breast adenocarcinoma cell lines	1487:1520	T breast adenocarcinoma cell lines	1487:1520	T breast adenocarcinoma cell lines, respectively.					
37352956	12	2	theme	dye	1621:1623	arg1	nanocomposite					1580:1592	the newly synthesized nanocomposite	1558:1592	the newly synthesized nanocomposite is a potent photocatalyst	1558:1618	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
37352956	12	2	theme	dye	1621:1623	arg1	agent					1643:1647	dye pollution removal agent	1621:1647	dye pollution removal agent	1621:1647	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
37352956	1	3	theme	different	253:261	arg1	fields					263:268	different fields	253:268	different fields	253:268	Nanocomposites have gained attention due to their variety of applications in different fields.					
37352956	8	4	theme	electrochemical	1083:1097	arg1	sensor					1099:1104	a potent electrochemical sensor	1074:1104	a potent electrochemical sensor to determine glucose	1074:1125	Ni/Zn@orange/chitosan was found as a potent electrochemical sensor to determine glucose.					
37352956	8	4	theme	electrochemical	1083:1097	arg1	orange/chitosan					1045:1059	Ni/Zn@orange/chitosan	1039:1059	Ni/Zn@orange/chitosan	1039:1059	Ni/Zn@orange/chitosan was found as a potent electrochemical sensor to determine glucose.					
37352956	12	5	dep	nanocomposite	1580:1592	arg1	photocatalyst					1606:1618	a potent photocatalyst	1597:1618	a potent photocatalyst	1597:1618	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
37352956	2	6	theme	nanocomposite	341:353	arg1	synthesis					314:322	a green synthesis	306:322	a green synthesis of a bi-metallic nanocomposite of nickel and zinc	306:372	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	8	7	theme	@	1044:1044	arg1	sensor					1099:1104	a potent electrochemical sensor	1074:1104	a potent electrochemical sensor to determine glucose	1074:1125	Ni/Zn@orange/chitosan was found as a potent electrochemical sensor to determine glucose.					
37352956	8	7	theme	@	1044:1044	arg1	orange/chitosan					1045:1059	Ni/Zn@orange/chitosan	1039:1059	Ni/Zn@orange/chitosan	1039:1059	Ni/Zn@orange/chitosan was found as a potent electrochemical sensor to determine glucose.					
37352956	9	8	theme	MTT	1246:1248	arg1	way					1250:1252	MTT way	1246:1252	MTT way	1246:1252	In the molecular and cellular section of the current research, the cells with composite nanoparticles were studied by MTT way about the anti-breast adenocarcinoma potentials malignant cell lines.					
37352956	0	9	theme	pollution	92:100	arg1	removal					102:108	pollution removal	92:108	pollution removal	92:108	A new formulation of Ni/Zn bi-metallic nanocomposite and evaluation of its applications for pollution removal, photocatalytic, electrochemical sensing, and anti-breast cancer.					
37352956	7	10	theme	great	934:938	arg1	activity					955:962	great photocatalytic activity	934:962	great photocatalytic activity	934:962	Ni/Zn@orange/chitosan also exhibited great photocatalytic activity in synthesizing benzimidazole using benzyl alcohol and o-phenylenediamine.					
37352956	5	11	theme	particle	657:664	arg1	size					672:675	a particle range size	655:675	a particle range size of 17.34-90.51 nm	655:693	The NPs were manufactured in spherical morphology with a particle range size of 17.34-90.51 nm.					
37352956	6	12	theme	aqueous	801:807	arg1	solution					809:816	an aqueous solution	798:816	an aqueous solution	798:816	Ni/Zn@orange/chitosan showed an acceptable ability to remove dyes of Congo red and methyl orange from an aqueous solution after 80 min furthermore, it uptaking the drug mefenamic acid from a solution.					
37352956	12	13	theme	removal	1635:1641	arg1	nanocomposite					1580:1592	the newly synthesized nanocomposite	1558:1592	the newly synthesized nanocomposite is a potent photocatalyst	1558:1618	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
37352956	12	13	theme	removal	1635:1641	arg1	agent					1643:1647	dye pollution removal agent	1621:1647	dye pollution removal agent	1621:1647	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
37352956	7	14	theme	benzyl	1000:1005	arg1	alcohol					1007:1013	benzyl alcohol	1000:1013	benzyl alcohol	1000:1013	Ni/Zn@orange/chitosan also exhibited great photocatalytic activity in synthesizing benzimidazole using benzyl alcohol and o-phenylenediamine.					
37352956	5	15	theme	range	666:670	arg1	size					672:675	a particle range size	655:675	a particle range size of 17.34-90.51 nm	655:693	The NPs were manufactured in spherical morphology with a particle range size of 17.34-90.51 nm.					
37352956	7	16	theme	photocatalytic	940:953	arg1	activity					955:962	great photocatalytic activity	934:962	great photocatalytic activity	934:962	Ni/Zn@orange/chitosan also exhibited great photocatalytic activity in synthesizing benzimidazole using benzyl alcohol and o-phenylenediamine.					
37352956	11	17	theme	adenocarcinoma	1496:1509	arg1	lines					1516:1520	T breast adenocarcinoma cell lines	1487:1520	T breast adenocarcinoma cell lines	1487:1520	T breast adenocarcinoma cell lines, respectively.					
37352956	4	18	theme	@	582:582	arg1	orange/chitosan					583:597	Ni/Zn@orange/chitosan	577:597	Ni/Zn@orange/chitosan	577:597	We have examined various applications for Ni/Zn@orange/chitosan.					
37352956	12	19	theme	potent	1599:1604	arg1	photocatalyst					1606:1618	a potent photocatalyst	1597:1618	a potent photocatalyst	1597:1618	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
37352956	9	20	theme	composite	1206:1214	arg1	nanoparticles					1216:1228	composite nanoparticles	1206:1228	composite nanoparticles	1206:1228	In the molecular and cellular section of the current research, the cells with composite nanoparticles were studied by MTT way about the anti-breast adenocarcinoma potentials malignant cell lines.					
37352956	3	21	theme	different	513:521	arg1	techniques					523:532	different techniques	513:532	different techniques	513:532	The nanocomposite was characterized using different techniques.					
37352956	2	22	theme	aqueous	383:389	arg1	extract					391:397	an aqueous extract	380:397	an aqueous extract of Citrus sinensis	380:416	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	0	23	theme	nanocomposite	39:51	arg1	formulation					6:16	A new formulation	0:16	A new formulation of Ni/Zn bi-metallic nanocomposite	0:51	A new formulation of Ni/Zn bi-metallic nanocomposite and evaluation of its applications for pollution removal, photocatalytic, electrochemical sensing, and anti-breast cancer.					
37352956	0	23	theme	nanocomposite	39:51	arg1	evaluation					57:66	evaluation	57:66	evaluation of its applications for pollution removal, photocatalytic, electrochemical sensing, and anti-breast cancer	57:173	A new formulation of Ni/Zn bi-metallic nanocomposite and evaluation of its applications for pollution removal, photocatalytic, electrochemical sensing, and anti-breast cancer.					
37352956	2	24	theme	@	452:452	arg1	orange/chitosan					453:467	Ni/Zn@orange/chitosan	447:467	Ni/Zn@orange/chitosan	447:467	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	2	24	theme	@	452:452	arg1	chitosan					437:444	chitosan	437:444	chitosan (Ni/Zn@orange/chitosan)	437:468	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	6	25	theme	orange	786:791	arg1	dyes					757:760	dyes	757:760	dyes of Congo red and methyl orange from an aqueous solution	757:816	Ni/Zn@orange/chitosan showed an acceptable ability to remove dyes of Congo red and methyl orange from an aqueous solution after 80 min furthermore, it uptaking the drug mefenamic acid from a solution.					
37352956	6	26	theme	@	701:701	arg1	orange/chitosan					702:716	Ni/Zn@orange/chitosan	696:716	Ni/Zn@orange/chitosan	696:716	Ni/Zn@orange/chitosan showed an acceptable ability to remove dyes of Congo red and methyl orange from an aqueous solution after 80 min furthermore, it uptaking the drug mefenamic acid from a solution.					
37352956	0	27	theme	new	2:4	arg1	formulation					6:16	A new formulation	0:16	A new formulation of Ni/Zn bi-metallic nanocomposite	0:51	A new formulation of Ni/Zn bi-metallic nanocomposite and evaluation of its applications for pollution removal, photocatalytic, electrochemical sensing, and anti-breast cancer.					
37352956	6	28	theme	mefenamic	865:873	arg1	acid					875:878	the drug mefenamic acid	856:878	the drug mefenamic acid	856:878	Ni/Zn@orange/chitosan showed an acceptable ability to remove dyes of Congo red and methyl orange from an aqueous solution after 80 min furthermore, it uptaking the drug mefenamic acid from a solution.					
37352956	5	29	theme	spherical	629:637	arg1	morphology					639:648	spherical morphology	629:648	spherical morphology with a particle range size of 17.34-90.51 nm	629:693	The NPs were manufactured in spherical morphology with a particle range size of 17.34-90.51 nm.					
37352956	6	30	theme	methyl	779:784	arg1	orange					786:791	methyl orange	779:791	methyl orange	779:791	Ni/Zn@orange/chitosan showed an acceptable ability to remove dyes of Congo red and methyl orange from an aqueous solution after 80 min furthermore, it uptaking the drug mefenamic acid from a solution.					
37352956	2	31	theme	green	308:312	arg1	synthesis					314:322	a green synthesis	306:322	a green synthesis of a bi-metallic nanocomposite of nickel and zinc	306:372	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	6	32	theme	Ni/Zn	696:700	arg1	orange/chitosan					702:716	Ni/Zn@orange/chitosan	696:716	Ni/Zn@orange/chitosan	696:716	Ni/Zn@orange/chitosan showed an acceptable ability to remove dyes of Congo red and methyl orange from an aqueous solution after 80 min furthermore, it uptaking the drug mefenamic acid from a solution.					
37352956	6	33	from	solution	809:816	arg1	dyes					757:760	dyes	757:760	dyes of Congo red and methyl orange from an aqueous solution	757:816	Ni/Zn@orange/chitosan showed an acceptable ability to remove dyes of Congo red and methyl orange from an aqueous solution after 80 min furthermore, it uptaking the drug mefenamic acid from a solution.					
37352956	1	34	from	fields	263:268	arg1	applications					237:248	applications	237:248	applications in different fields	237:268	Nanocomposites have gained attention due to their variety of applications in different fields.					
37352956	1	34	from	fields	263:268	arg1	variety					226:232	their variety	220:232	their variety of applications in different fields	220:268	Nanocomposites have gained attention due to their variety of applications in different fields.					
37352956	8	35	theme	potent	1076:1081	arg1	sensor					1099:1104	a potent electrochemical sensor	1074:1104	a potent electrochemical sensor to determine glucose	1074:1125	Ni/Zn@orange/chitosan was found as a potent electrochemical sensor to determine glucose.					
37352956	8	35	theme	potent	1076:1081	arg1	orange/chitosan					1045:1059	Ni/Zn@orange/chitosan	1039:1059	Ni/Zn@orange/chitosan	1039:1059	Ni/Zn@orange/chitosan was found as a potent electrochemical sensor to determine glucose.					
37352956	8	36	theme	Ni/Zn	1039:1043	arg1	sensor					1099:1104	a potent electrochemical sensor	1074:1104	a potent electrochemical sensor to determine glucose	1074:1125	Ni/Zn@orange/chitosan was found as a potent electrochemical sensor to determine glucose.					
37352956	8	36	theme	Ni/Zn	1039:1043	arg1	orange/chitosan					1045:1059	Ni/Zn@orange/chitosan	1039:1059	Ni/Zn@orange/chitosan	1039:1059	Ni/Zn@orange/chitosan was found as a potent electrochemical sensor to determine glucose.					
37352956	9	37	theme	anti-breast	1264:1274	arg1	lines					1317:1321	the anti-breast adenocarcinoma potentials malignant cell lines	1260:1321	the anti-breast adenocarcinoma potentials malignant cell lines	1260:1321	In the molecular and cellular section of the current research, the cells with composite nanoparticles were studied by MTT way about the anti-breast adenocarcinoma potentials malignant cell lines.					
37352956	10	38	theme	nanoparticles	1346:1358	arg1	320					1365:1367	320	1365:1367	320	1365:1367	The IC50 of composite nanoparticles were 320, 460, 328, 500, 325, 379, 350, and 396 μg/mL concering RBA, NMU, SK-BR-3, CAMA-1, MCF7, AU565, MDA-MB-468, and Hs 281.					
37352956	10	38	theme	nanoparticles	1346:1358	arg1	IC50					1328:1331	The IC50	1324:1331	The IC50 of composite nanoparticles	1324:1358	The IC50 of composite nanoparticles were 320, 460, 328, 500, 325, 379, 350, and 396 μg/mL concering RBA, NMU, SK-BR-3, CAMA-1, MCF7, AU565, MDA-MB-468, and Hs 281.					
37352956	2	39	theme	zinc	369:372	arg1	zinc					369:372	zinc	369:372	zinc	369:372	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	2	39	theme	zinc	369:372	arg1	nickel					358:363	nickel	358:363	nickel	358:363	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	2	39	theme	zinc	369:372	arg1	nanocomposite					341:353	a bi-metallic nanocomposite	327:353	a bi-metallic nanocomposite of nickel and zinc	327:372	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	11	40	theme	T	1487:1487	arg1	lines					1516:1520	T breast adenocarcinoma cell lines	1487:1520	T breast adenocarcinoma cell lines	1487:1520	T breast adenocarcinoma cell lines, respectively.					
37352956	6	41	theme	red	771:773	arg1	dyes					757:760	dyes	757:760	dyes of Congo red and methyl orange from an aqueous solution	757:816	Ni/Zn@orange/chitosan showed an acceptable ability to remove dyes of Congo red and methyl orange from an aqueous solution after 80 min furthermore, it uptaking the drug mefenamic acid from a solution.					
37352956	10	42	theme	composite	1336:1344	arg1	nanoparticles					1346:1358	composite nanoparticles	1336:1358	composite nanoparticles	1336:1358	The IC50 of composite nanoparticles were 320, 460, 328, 500, 325, 379, 350, and 396 μg/mL concering RBA, NMU, SK-BR-3, CAMA-1, MCF7, AU565, MDA-MB-468, and Hs 281.					
37352956	2	43	theme	sinensis	409:416	arg1	extract					391:397	an aqueous extract	380:397	an aqueous extract of Citrus sinensis	380:416	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	11	44	theme	cell	1511:1514	arg1	lines					1516:1520	T breast adenocarcinoma cell lines	1487:1520	T breast adenocarcinoma cell lines	1487:1520	T breast adenocarcinoma cell lines, respectively.					
37352956	6	45	theme	drug	860:863	arg1	acid					875:878	the drug mefenamic acid	856:878	the drug mefenamic acid	856:878	Ni/Zn@orange/chitosan showed an acceptable ability to remove dyes of Congo red and methyl orange from an aqueous solution after 80 min furthermore, it uptaking the drug mefenamic acid from a solution.					
37352956	10	46	dep	320	1365:1367	arg1	460					1370:1372	460	1370:1372	460	1370:1372	The IC50 of composite nanoparticles were 320, 460, 328, 500, 325, 379, 350, and 396 μg/mL concering RBA, NMU, SK-BR-3, CAMA-1, MCF7, AU565, MDA-MB-468, and Hs 281.					
37352956	10	47	dep	460	1370:1372	arg1	328					1375:1377	328	1375:1377	328	1375:1377	The IC50 of composite nanoparticles were 320, 460, 328, 500, 325, 379, 350, and 396 μg/mL concering RBA, NMU, SK-BR-3, CAMA-1, MCF7, AU565, MDA-MB-468, and Hs 281.					
37352956	10	47	dep	460	1370:1372	arg1	325					1385:1387	325	1385:1387	325	1385:1387	The IC50 of composite nanoparticles were 320, 460, 328, 500, 325, 379, 350, and 396 μg/mL concering RBA, NMU, SK-BR-3, CAMA-1, MCF7, AU565, MDA-MB-468, and Hs 281.					
37352956	10	47	dep	460	1370:1372	arg1	concering					1414:1422	concering	1414:1422	concering RBA, NMU, SK-BR-3, CAMA-1, MCF7, AU565, MDA-MB-468, and Hs 281	1414:1485	The IC50 of composite nanoparticles were 320, 460, 328, 500, 325, 379, 350, and 396 μg/mL concering RBA, NMU, SK-BR-3, CAMA-1, MCF7, AU565, MDA-MB-468, and Hs 281.					
37352956	10	47	dep	460	1370:1372	arg1	500					1380:1382	500	1380:1382	500	1380:1382	The IC50 of composite nanoparticles were 320, 460, 328, 500, 325, 379, 350, and 396 μg/mL concering RBA, NMU, SK-BR-3, CAMA-1, MCF7, AU565, MDA-MB-468, and Hs 281.					
37352956	2	48	theme	Citrus	402:407	arg1	sinensis					409:416	Citrus sinensis	402:416	Citrus sinensis	402:416	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	0	49	theme	bi-metallic	27:37	arg1	nanocomposite					39:51	Ni/Zn bi-metallic nanocomposite	21:51	Ni/Zn bi-metallic nanocomposite	21:51	A new formulation of Ni/Zn bi-metallic nanocomposite and evaluation of its applications for pollution removal, photocatalytic, electrochemical sensing, and anti-breast cancer.					
37352956	4	50	theme	various	552:558	arg1	applications					560:571	various applications	552:571	various applications for Ni/Zn@orange/chitosan	552:597	We have examined various applications for Ni/Zn@orange/chitosan.					
37352956	9	51	theme	molecular	1135:1143	arg1	section					1158:1164	the molecular and cellular section	1131:1164	the molecular and cellular section of the current research	1131:1188	In the molecular and cellular section of the current research, the cells with composite nanoparticles were studied by MTT way about the anti-breast adenocarcinoma potentials malignant cell lines.					
37352956	2	52	theme	Ni/Zn	447:451	arg1	orange/chitosan					453:467	Ni/Zn@orange/chitosan	447:467	Ni/Zn@orange/chitosan	447:467	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	2	52	theme	Ni/Zn	447:451	arg1	chitosan					437:444	chitosan	437:444	chitosan (Ni/Zn@orange/chitosan)	437:468	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	0	53	theme	Ni/Zn	21:25	arg1	nanocomposite					39:51	Ni/Zn bi-metallic nanocomposite	21:51	Ni/Zn bi-metallic nanocomposite	21:51	A new formulation of Ni/Zn bi-metallic nanocomposite and evaluation of its applications for pollution removal, photocatalytic, electrochemical sensing, and anti-breast cancer.					
37352956	1	54	from	applications	237:248	arg1	fields					263:268	different fields	253:268	different fields	253:268	Nanocomposites have gained attention due to their variety of applications in different fields.					
37352956	12	55	theme	synthesized	1568:1578	arg1	nanocomposite					1580:1592	the newly synthesized nanocomposite	1558:1592	the newly synthesized nanocomposite is a potent photocatalyst	1558:1618	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
37352956	12	55	theme	synthesized	1568:1578	arg1	agent					1643:1647	dye pollution removal agent	1621:1647	dye pollution removal agent	1621:1647	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
37352956	5	56	theme	17.34-90.51 nm	680:693	arg1	size					672:675	a particle range size	655:675	a particle range size of 17.34-90.51 nm	655:693	The NPs were manufactured in spherical morphology with a particle range size of 17.34-90.51 nm.					
37352956	2	57	theme	bi-metallic	329:339	arg1	zinc					369:372	zinc	369:372	zinc	369:372	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	2	57	theme	bi-metallic	329:339	arg1	nickel					358:363	nickel	358:363	nickel	358:363	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	2	57	theme	bi-metallic	329:339	arg1	nanocomposite					341:353	a bi-metallic nanocomposite	327:353	a bi-metallic nanocomposite of nickel and zinc	327:372	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	0	58	theme	electrochemical	127:141	arg1	sensing					143:149	electrochemical sensing	127:149	electrochemical sensing	127:149	A new formulation of Ni/Zn bi-metallic nanocomposite and evaluation of its applications for pollution removal, photocatalytic, electrochemical sensing, and anti-breast cancer.					
37352956	12	59	theme	new	1668:1670	arg1	drug					1672:1675	an acceptable new drug	1654:1675	an acceptable new drug	1654:1675	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
37352956	2	60	theme	chitosan	437:444	arg1	presence					425:432	the presence	421:432	the presence of chitosan (Ni/Zn@orange/chitosan)	421:468	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	5	61	with	morphology	639:648	arg1	size					672:675	a particle range size	655:675	a particle range size of 17.34-90.51 nm	655:693	The NPs were manufactured in spherical morphology with a particle range size of 17.34-90.51 nm.					
37352956	7	62	theme	Ni/Zn	897:901	arg1	orange/chitosan					903:917	Ni/Zn@orange/chitosan	897:917	Ni/Zn@orange/chitosan	897:917	Ni/Zn@orange/chitosan also exhibited great photocatalytic activity in synthesizing benzimidazole using benzyl alcohol and o-phenylenediamine.					
37352956	9	63	with	cells	1195:1199	arg1	nanoparticles					1216:1228	composite nanoparticles	1206:1228	composite nanoparticles	1206:1228	In the molecular and cellular section of the current research, the cells with composite nanoparticles were studied by MTT way about the anti-breast adenocarcinoma potentials malignant cell lines.					
37352956	1	64	from	variety	226:232	arg1	fields					263:268	different fields	253:268	different fields	253:268	Nanocomposites have gained attention due to their variety of applications in different fields.					
37352956	9	65	theme	adenocarcinoma	1276:1289	arg1	lines					1317:1321	the anti-breast adenocarcinoma potentials malignant cell lines	1260:1321	the anti-breast adenocarcinoma potentials malignant cell lines	1260:1321	In the molecular and cellular section of the current research, the cells with composite nanoparticles were studied by MTT way about the anti-breast adenocarcinoma potentials malignant cell lines.					
37352956	12	66	theme	breast	1686:1691	arg1	cancer					1693:1698	breast cancer	1686:1698	breast cancer	1686:1698	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
37352956	7	67	theme	@	902:902	arg1	orange/chitosan					903:917	Ni/Zn@orange/chitosan	897:917	Ni/Zn@orange/chitosan	897:917	Ni/Zn@orange/chitosan also exhibited great photocatalytic activity in synthesizing benzimidazole using benzyl alcohol and o-phenylenediamine.					
37352956	9	68	theme	cellular	1149:1156	arg1	section					1158:1164	the molecular and cellular section	1131:1164	the molecular and cellular section of the current research	1131:1188	In the molecular and cellular section of the current research, the cells with composite nanoparticles were studied by MTT way about the anti-breast adenocarcinoma potentials malignant cell lines.					
37352956	0	69	theme	anti-breast	156:166	arg1	cancer					168:173	anti-breast cancer	156:173	anti-breast cancer	156:173	A new formulation of Ni/Zn bi-metallic nanocomposite and evaluation of its applications for pollution removal, photocatalytic, electrochemical sensing, and anti-breast cancer.					
37352956	9	70	theme	potentials	1291:1300	arg1	lines					1317:1321	the anti-breast adenocarcinoma potentials malignant cell lines	1260:1321	the anti-breast adenocarcinoma potentials malignant cell lines	1260:1321	In the molecular and cellular section of the current research, the cells with composite nanoparticles were studied by MTT way about the anti-breast adenocarcinoma potentials malignant cell lines.					
37352956	9	71	theme	research	1181:1188	arg1	section					1158:1164	the molecular and cellular section	1131:1164	the molecular and cellular section of the current research	1131:1188	In the molecular and cellular section of the current research, the cells with composite nanoparticles were studied by MTT way about the anti-breast adenocarcinoma potentials malignant cell lines.					
37352956	4	72	theme	Ni/Zn	577:581	arg1	orange/chitosan					583:597	Ni/Zn@orange/chitosan	577:597	Ni/Zn@orange/chitosan	577:597	We have examined various applications for Ni/Zn@orange/chitosan.					
37352956	12	73	theme	acceptable	1657:1666	arg1	drug					1672:1675	an acceptable new drug	1654:1675	an acceptable new drug	1654:1675	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
37352956	9	74	theme	malignant	1302:1310	arg1	lines					1317:1321	the anti-breast adenocarcinoma potentials malignant cell lines	1260:1321	the anti-breast adenocarcinoma potentials malignant cell lines	1260:1321	In the molecular and cellular section of the current research, the cells with composite nanoparticles were studied by MTT way about the anti-breast adenocarcinoma potentials malignant cell lines.					
37352956	9	75	theme	current	1173:1179	arg1	research					1181:1188	the current research	1169:1188	the current research	1169:1188	In the molecular and cellular section of the current research, the cells with composite nanoparticles were studied by MTT way about the anti-breast adenocarcinoma potentials malignant cell lines.					
37352956	1	76	theme	applications	237:248	arg1	applications					237:248	applications	237:248	applications in different fields	237:268	Nanocomposites have gained attention due to their variety of applications in different fields.					
37352956	1	76	theme	applications	237:248	arg1	variety					226:232	their variety	220:232	their variety of applications in different fields	220:268	Nanocomposites have gained attention due to their variety of applications in different fields.					
37352956	9	77	theme	cell	1312:1315	arg1	lines					1317:1321	the anti-breast adenocarcinoma potentials malignant cell lines	1260:1321	the anti-breast adenocarcinoma potentials malignant cell lines	1260:1321	In the molecular and cellular section of the current research, the cells with composite nanoparticles were studied by MTT way about the anti-breast adenocarcinoma potentials malignant cell lines.					
37352956	6	78	theme	acceptable	728:737	arg1	ability					739:745	an acceptable ability	725:745	an acceptable ability to remove dyes of Congo red and methyl orange from an aqueous solution after 80 min furthermore	725:841	Ni/Zn@orange/chitosan showed an acceptable ability to remove dyes of Congo red and methyl orange from an aqueous solution after 80 min furthermore, it uptaking the drug mefenamic acid from a solution.					
37352956	2	79	theme	nickel	358:363	arg1	zinc					369:372	zinc	369:372	zinc	369:372	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	2	79	theme	nickel	358:363	arg1	nickel					358:363	nickel	358:363	nickel	358:363	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	2	79	theme	nickel	358:363	arg1	nanocomposite					341:353	a bi-metallic nanocomposite	327:353	a bi-metallic nanocomposite of nickel and zinc	327:372	In this research, we have reported a green synthesis of a bi-metallic nanocomposite of nickel and zinc using an aqueous extract of Citrus sinensis in the presence of chitosan (Ni/Zn@orange/chitosan).					
37352956	12	80	theme	pollution	1625:1633	arg1	nanocomposite					1580:1592	the newly synthesized nanocomposite	1558:1592	the newly synthesized nanocomposite is a potent photocatalyst	1558:1618	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
37352956	12	80	theme	pollution	1625:1633	arg1	agent					1643:1647	dye pollution removal agent	1621:1647	dye pollution removal agent	1621:1647	The results revealed the newly synthesized nanocomposite is a potent photocatalyst, dye pollution removal agent, and an acceptable new drug to treat breast cancer.					
35500406	7	0	theme	substitution	1081:1092	arg1	affinity					1094:1101	substitution affinity	1081:1101	substitution affinity	1081:1101	In short, the molecular simulation results theoretically proved that heavy metal ions owned substitution affinity with lignosulfonate.					
35500406	1	1	theme	paper	185:189	arg1	fluorescence					229:240	an interfering fluorescence	214:240	an interfering fluorescence	214:240	Auto-fluorescence of cellulose paper is often considered as an interfering fluorescence, which directly impedes the cellulose paper as a substrate material.					
35500406	1	1	theme	paper	185:189	arg1	Auto-fluorescence					154:170	Auto-fluorescence	154:170	Auto-fluorescence of cellulose paper	154:189	Auto-fluorescence of cellulose paper is often considered as an interfering fluorescence, which directly impedes the cellulose paper as a substrate material.					
35500406	5	2	theme	different	813:821	arg1	responses					836:844	different fluorescence responses	813:844	different fluorescence responses to three heavy metal ions in water sample	813:886	Besides, the paper chip exhibited different fluorescence responses to three heavy metal ions in water sample.					
35500406	0	3	theme	enhancement	95:105	arg1	behavior					107:114	fluorescence enhancement behavior	82:114	fluorescence enhancement behavior for three heavy metal ions detection	82:151	Auto-fluorescence of cellulose paper with spatial solid phrase dispersion-induced fluorescence enhancement behavior for three heavy metal ions detection.					
35500406	4	4	theme	fluorescence	754:765	arg1	properties					767:776	fluorescence properties	754:776	fluorescence properties	754:776	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	3	5	theme	solid	506:510	arg1	enhancement					551:561	its spatial solid phrase dispersion-induced fluorescence enhancement	494:561	its spatial solid phrase dispersion-induced fluorescence enhancement behavior	494:570	Surprisingly, its spatial solid phrase dispersion-induced fluorescence enhancement behavior was found.					
35500406	7	6	theme	molecular	1003:1011	arg1	results					1024:1030	the molecular simulation results	999:1030	the molecular simulation results	999:1030	In short, the molecular simulation results theoretically proved that heavy metal ions owned substitution affinity with lignosulfonate.					
35500406	1	7	theme	substrate	291:299	arg1	material					301:308	a substrate material	289:308	a substrate material	289:308	Auto-fluorescence of cellulose paper is often considered as an interfering fluorescence, which directly impedes the cellulose paper as a substrate material.					
35500406	1	7	theme	substrate	291:299	arg1	paper					280:284	the cellulose paper	266:284	the cellulose paper	266:284	Auto-fluorescence of cellulose paper is often considered as an interfering fluorescence, which directly impedes the cellulose paper as a substrate material.					
35500406	0	8	theme	metal	132:136	arg1	ions					138:141	three heavy metal ions	120:141	three heavy metal ions detection	120:151	Auto-fluorescence of cellulose paper with spatial solid phrase dispersion-induced fluorescence enhancement behavior for three heavy metal ions detection.					
35500406	8	9	theme	paper-based	1260:1270	arg1	chips					1272:1276	paper-based chips	1260:1276	paper-based chips	1260:1276	Ultimately, this study was the first attempt to utilize paper-based auto-fluorescence, which could better accelerate the development of paper-based chips.					
35500406	0	10	theme	heavy	126:130	arg1	ions					138:141	three heavy metal ions	120:141	three heavy metal ions detection	120:151	Auto-fluorescence of cellulose paper with spatial solid phrase dispersion-induced fluorescence enhancement behavior for three heavy metal ions detection.					
35500406	5	11	theme	heavy	855:859	arg1	ions					867:870	three heavy metal ions	849:870	three heavy metal ions in water sample	849:886	Besides, the paper chip exhibited different fluorescence responses to three heavy metal ions in water sample.					
35500406	4	12	theme	prepared	658:665	arg1	chip					697:700	the prepared ratiometric fluorescent paper chip	654:700	the prepared ratiometric fluorescent paper chip	654:700	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	5	13	theme	water	875:879	arg1	sample					881:886	water sample	875:886	water sample	875:886	Besides, the paper chip exhibited different fluorescence responses to three heavy metal ions in water sample.					
35500406	5	14	theme	fluorescence	823:834	arg1	responses					836:844	different fluorescence responses	813:844	different fluorescence responses to three heavy metal ions in water sample	813:886	Besides, the paper chip exhibited different fluorescence responses to three heavy metal ions in water sample.					
35500406	4	15	mod	modified	609:616	arg3	dots					644:647	Mn-doped ZnS quantum dots	623:647	Mn-doped ZnS quantum dots	623:647	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	4	15	mod	modified	609:616	arg1	paper					599:603	cellulose paper	589:603	cellulose paper	589:603	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	4	16	theme	Mn-doped	623:630	arg1	dots					644:647	Mn-doped ZnS quantum dots	623:647	Mn-doped ZnS quantum dots	623:647	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	5	17	theme	paper	792:796	arg1	chip					798:801	the paper chip	788:801	the paper chip	788:801	Besides, the paper chip exhibited different fluorescence responses to three heavy metal ions in water sample.					
35500406	0	18	theme	paper	31:35	arg1	Auto-fluorescence					0:16	Auto-fluorescence	0:16	Auto-fluorescence of cellulose paper with spatial solid phrase	0:61	Auto-fluorescence of cellulose paper with spatial solid phrase dispersion-induced fluorescence enhancement behavior for three heavy metal ions detection.					
35500406	3	19	theme	phrase	512:517	arg1	enhancement					551:561	its spatial solid phrase dispersion-induced fluorescence enhancement	494:561	its spatial solid phrase dispersion-induced fluorescence enhancement behavior	494:570	Surprisingly, its spatial solid phrase dispersion-induced fluorescence enhancement behavior was found.					
35500406	0	20	theme	cellulose	21:29	arg1	paper					31:35	cellulose paper	21:35	cellulose paper	21:35	Auto-fluorescence of cellulose paper with spatial solid phrase dispersion-induced fluorescence enhancement behavior for three heavy metal ions detection.					
35500406	4	21	theme	quantum	636:642	arg1	dots					644:647	Mn-doped ZnS quantum dots	623:647	Mn-doped ZnS quantum dots	623:647	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	0	22	theme	ions	138:141	arg1	detection					143:151	three heavy metal ions detection	120:151	three heavy metal ions detection	120:151	Auto-fluorescence of cellulose paper with spatial solid phrase dispersion-induced fluorescence enhancement behavior for three heavy metal ions detection.					
35500406	3	23	theme	dispersion-induced	519:536	arg1	enhancement					551:561	its spatial solid phrase dispersion-induced fluorescence enhancement	494:561	its spatial solid phrase dispersion-induced fluorescence enhancement behavior	494:570	Surprisingly, its spatial solid phrase dispersion-induced fluorescence enhancement behavior was found.					
35500406	0	24	theme	spatial	42:48	arg1	phrase					56:61	spatial solid phrase	42:61	spatial solid phrase	42:61	Auto-fluorescence of cellulose paper with spatial solid phrase dispersion-induced fluorescence enhancement behavior for three heavy metal ions detection.					
35500406	4	25	theme	ZnS	632:634	arg1	dots					644:647	Mn-doped ZnS quantum dots	623:647	Mn-doped ZnS quantum dots	623:647	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	4	26	theme	good	706:709	arg1	performances					711:722	good performances	706:722	good performances	706:722	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	3	27	theme	spatial	498:504	arg1	enhancement					551:561	its spatial solid phrase dispersion-induced fluorescence enhancement	494:561	its spatial solid phrase dispersion-induced fluorescence enhancement behavior	494:570	Surprisingly, its spatial solid phrase dispersion-induced fluorescence enhancement behavior was found.					
35500406	5	28	theme	metal	861:865	arg1	ions					867:870	three heavy metal ions	849:870	three heavy metal ions in water sample	849:886	Besides, the paper chip exhibited different fluorescence responses to three heavy metal ions in water sample.					
35500406	8	29	theme	chips	1272:1276	arg1	development					1245:1255	the development	1241:1255	the development of paper-based chips	1241:1276	Ultimately, this study was the first attempt to utilize paper-based auto-fluorescence, which could better accelerate the development of paper-based chips.					
35500406	7	30	theme	metal	1064:1068	arg1	ions					1070:1073	heavy metal ions	1058:1073	heavy metal ions	1058:1073	In short, the molecular simulation results theoretically proved that heavy metal ions owned substitution affinity with lignosulfonate.					
35500406	2	31	theme	main	446:449	arg1	source					451:456	the main source	442:456	the main source of auto-fluorescence	442:477	This paper creatively explored the composition and properties of auto-fluorescence, and lignosulfonate was primarily speculated as the main source of auto-fluorescence.					
35500406	2	31	theme	main	446:449	arg1	lignosulfonate					399:412	lignosulfonate	399:412	lignosulfonate	399:412	This paper creatively explored the composition and properties of auto-fluorescence, and lignosulfonate was primarily speculated as the main source of auto-fluorescence.					
35500406	4	32	theme	cellulose	589:597	arg1	paper					599:603	cellulose paper	589:603	cellulose paper	589:603	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	4	33	contain	has	702:704	arg1	chip					697:700	the prepared ratiometric fluorescent paper chip	654:700	the prepared ratiometric fluorescent paper chip	654:700	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	4	33	contain	has	702:704	arg2	performances					711:722	good performances	706:722	good performances	706:722	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	2	34	theme	auto-fluorescence	376:392	arg1	properties					362:371	properties	362:371	properties	362:371	This paper creatively explored the composition and properties of auto-fluorescence, and lignosulfonate was primarily speculated as the main source of auto-fluorescence.					
35500406	2	34	theme	auto-fluorescence	376:392	arg1	composition					346:356	composition	346:356	composition	346:356	This paper creatively explored the composition and properties of auto-fluorescence, and lignosulfonate was primarily speculated as the main source of auto-fluorescence.					
35500406	0	35	theme	solid	50:54	arg1	phrase					56:61	spatial solid phrase	42:61	spatial solid phrase	42:61	Auto-fluorescence of cellulose paper with spatial solid phrase dispersion-induced fluorescence enhancement behavior for three heavy metal ions detection.					
35500406	8	36	theme	first	1155:1159	arg1	attempt					1161:1167	the first attempt	1151:1167	the first attempt to utilize paper-based auto-fluorescence, which could better accelerate the development of paper-based chips	1151:1276	Ultimately, this study was the first attempt to utilize paper-based auto-fluorescence, which could better accelerate the development of paper-based chips.					
35500406	8	36	theme	first	1155:1159	arg1	study					1141:1145	this study	1136:1145	this study	1136:1145	Ultimately, this study was the first attempt to utilize paper-based auto-fluorescence, which could better accelerate the development of paper-based chips.					
35500406	6	37	theme	detection	902:910	arg1	limit					893:897	The limit	889:897	The limit of detection for Cd2+, Hg2+ and Pb2+	889:934	The limit of detection for Cd2+, Hg2+ and Pb2+ reached 1.61 nM, 0.01 nM, and 0.02 nM, respectively.					
35500406	0	38	with	Auto-fluorescence	0:16	arg1	phrase					56:61	spatial solid phrase	42:61	spatial solid phrase	42:61	Auto-fluorescence of cellulose paper with spatial solid phrase dispersion-induced fluorescence enhancement behavior for three heavy metal ions detection.					
35500406	2	39	dep	composition	346:356	arg1	the					342:344	the	342:344	the	342:344	This paper creatively explored the composition and properties of auto-fluorescence, and lignosulfonate was primarily speculated as the main source of auto-fluorescence.					
35500406	4	40	theme	ratiometric	667:677	arg1	chip					697:700	the prepared ratiometric fluorescent paper chip	654:700	the prepared ratiometric fluorescent paper chip	654:700	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	7	41	theme	heavy	1058:1062	arg1	ions					1070:1073	heavy metal ions	1058:1073	heavy metal ions	1058:1073	In short, the molecular simulation results theoretically proved that heavy metal ions owned substitution affinity with lignosulfonate.					
35500406	3	42	theme	enhancement	551:561	arg1	behavior					563:570	its spatial solid phrase dispersion-induced fluorescence enhancement behavior	494:570	its spatial solid phrase dispersion-induced fluorescence enhancement behavior	494:570	Surprisingly, its spatial solid phrase dispersion-induced fluorescence enhancement behavior was found.					
35500406	3	43	theme	fluorescence	538:549	arg1	enhancement					551:561	its spatial solid phrase dispersion-induced fluorescence enhancement	494:561	its spatial solid phrase dispersion-induced fluorescence enhancement behavior	494:570	Surprisingly, its spatial solid phrase dispersion-induced fluorescence enhancement behavior was found.					
35500406	0	44	theme	fluorescence	82:93	arg1	behavior					107:114	fluorescence enhancement behavior	82:114	fluorescence enhancement behavior for three heavy metal ions detection	82:151	Auto-fluorescence of cellulose paper with spatial solid phrase dispersion-induced fluorescence enhancement behavior for three heavy metal ions detection.					
35500406	1	45	theme	interfering	217:227	arg1	fluorescence					229:240	an interfering fluorescence	214:240	an interfering fluorescence	214:240	Auto-fluorescence of cellulose paper is often considered as an interfering fluorescence, which directly impedes the cellulose paper as a substrate material.					
35500406	1	45	theme	interfering	217:227	arg1	Auto-fluorescence					154:170	Auto-fluorescence	154:170	Auto-fluorescence of cellulose paper	154:189	Auto-fluorescence of cellulose paper is often considered as an interfering fluorescence, which directly impedes the cellulose paper as a substrate material.					
35500406	2	46	theme	auto-fluorescence	461:477	arg1	source					451:456	the main source	442:456	the main source of auto-fluorescence	442:477	This paper creatively explored the composition and properties of auto-fluorescence, and lignosulfonate was primarily speculated as the main source of auto-fluorescence.					
35500406	2	46	theme	auto-fluorescence	461:477	arg1	lignosulfonate					399:412	lignosulfonate	399:412	lignosulfonate	399:412	This paper creatively explored the composition and properties of auto-fluorescence, and lignosulfonate was primarily speculated as the main source of auto-fluorescence.					
35500406	4	47	theme	paper	691:695	arg1	chip					697:700	the prepared ratiometric fluorescent paper chip	654:700	the prepared ratiometric fluorescent paper chip	654:700	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	8	48	theme	paper-based	1180:1190	arg1	auto-fluorescence					1192:1208	paper-based auto-fluorescence	1180:1208	paper-based auto-fluorescence	1180:1208	Ultimately, this study was the first attempt to utilize paper-based auto-fluorescence, which could better accelerate the development of paper-based chips.					
35500406	5	49	from	ions	867:870	arg1	sample					881:886	water sample	875:886	water sample	875:886	Besides, the paper chip exhibited different fluorescence responses to three heavy metal ions in water sample.					
35500406	4	50	theme	fluorescent	679:689	arg1	chip					697:700	the prepared ratiometric fluorescent paper chip	654:700	the prepared ratiometric fluorescent paper chip	654:700	Then, cellulose paper was modified with Mn-doped ZnS quantum dots, and the prepared ratiometric fluorescent paper chip has good performances on morphology, stability, and fluorescence properties.					
35500406	7	51	theme	simulation	1013:1022	arg1	results					1024:1030	the molecular simulation results	999:1030	the molecular simulation results	999:1030	In short, the molecular simulation results theoretically proved that heavy metal ions owned substitution affinity with lignosulfonate.					
35500406	1	52	theme	cellulose	175:183	arg1	paper					185:189	cellulose paper	175:189	cellulose paper	175:189	Auto-fluorescence of cellulose paper is often considered as an interfering fluorescence, which directly impedes the cellulose paper as a substrate material.					
35500406	1	53	theme	cellulose	270:278	arg1	material					301:308	a substrate material	289:308	a substrate material	289:308	Auto-fluorescence of cellulose paper is often considered as an interfering fluorescence, which directly impedes the cellulose paper as a substrate material.					
35500406	1	53	theme	cellulose	270:278	arg1	paper					280:284	the cellulose paper	266:284	the cellulose paper	266:284	Auto-fluorescence of cellulose paper is often considered as an interfering fluorescence, which directly impedes the cellulose paper as a substrate material.					
35191679	0	0	theme	UV-Shielding	82:93	arg1	Efficacy					95:102	UV-Shielding Efficacy	82:102	UV-Shielding Efficacy	82:102	Starch-Based Composite Films with Enhanced Hydrophobicity, Thermal Stability, and UV-Shielding Efficacy Induced by Lignin Nanoparticles.					
35191679	7	1	theme	starch	1387:1392	arg1	film					1404:1407	the starch composite film	1383:1407	the starch composite film	1383:1407	What is more, the as-prepared LNP conferred strengthened thermal stability and ultraviolet blocking properties on the starch composite film.					
35191679	1	2	theme	lignin	172:177	arg1	utilization					157:167	Thehighly efficient utilization	137:167	Thehighly efficient utilization of lignin	137:177	Thehighly efficient utilization of lignin is of great importance for the development of the biorefinery industry.					
35191679	7	3	theme	ultraviolet	1348:1358	arg1	properties					1369:1378	ultraviolet blocking properties	1348:1378	ultraviolet blocking properties	1348:1378	What is more, the as-prepared LNP conferred strengthened thermal stability and ultraviolet blocking properties on the starch composite film.					
35191679	8	4	theme	composite	1531:1539	arg1	material					1541:1548	the composite material	1527:1548	the composite material with wide applications	1527:1571	The structural combination of the polymer film with LNPs holds the promise for providing advanced functionalities to the composite material with wide applications.					
35191679	6	5	theme	film	1263:1266	arg1	hydrophobicity					1234:1247	the enhanced hydrophobicity	1221:1247	the enhanced hydrophobicity of the starch film	1221:1266	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	3	6	theme	composite	626:634	arg1	film					636:639	starch/polyethylene oxide (PEO) composite film	594:639	starch/polyethylene oxide (PEO) composite film	594:639	In an attempt to endow composite materials with vital functionalities, the LNP was added to the starch film and the starch/polyvinyl alcohol (PVA) or starch/polyethylene oxide (PEO) composite film.					
35191679	5	7	theme	excellent	959:967	arg1	angle					983:987	an excellent water contact angle	956:987	an excellent water contact angle above 120°	956:998	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	3	8	theme	composite	467:475	arg1	materials					477:485	composite materials	467:485	composite materials with vital functionalities	467:512	In an attempt to endow composite materials with vital functionalities, the LNP was added to the starch film and the starch/polyvinyl alcohol (PVA) or starch/polyethylene oxide (PEO) composite film.					
35191679	5	9	theme	%	841:841	arg1	addition					848:855	1% (wt) addition	840:855	1% (wt) addition of LNPs	840:863	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	6	10	theme	starch	1256:1261	arg1	film					1263:1266	the starch film	1252:1266	the starch film	1252:1266	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	3	11	with	materials	477:485	arg1	functionalities					498:512	vital functionalities	492:512	vital functionalities	492:512	In an attempt to endow composite materials with vital functionalities, the LNP was added to the starch film and the starch/polyvinyl alcohol (PVA) or starch/polyethylene oxide (PEO) composite film.					
35191679	4	12	theme	films	716:720	arg1	performance					685:695	the hydrophobicity performance	666:695	the hydrophobicity performance of the synthesized films	666:720	The results showed that the hydrophobicity performance of the synthesized films was enhanced significantly.					
35191679	6	13	theme	rough	1053:1057	arg1	structures					1059:1068	some rough structures	1048:1068	some rough structures	1048:1068	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	4	14	theme	synthesized	704:714	arg1	films					716:720	the synthesized films	700:720	the synthesized films	700:720	The results showed that the hydrophobicity performance of the synthesized films was enhanced significantly.					
35191679	2	15	theme	lignin	280:285	arg1	LNP					301:303	LNP	301:303	LNP	301:303	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	2	15	theme	lignin	280:285	arg1	nanoparticle					287:298	a novel "core-shell" lignin nanoparticle	259:298	a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm	259:337	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	5	16	theme	LNPs	860:863	arg1	addition					848:855	1% (wt) addition	840:855	1% (wt) addition of LNPs	840:863	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	0	17	theme	Lignin	115:120	arg1	Nanoparticles					122:134	Lignin Nanoparticles	115:134	Lignin Nanoparticles	115:134	Starch-Based Composite Films with Enhanced Hydrophobicity, Thermal Stability, and UV-Shielding Efficacy Induced by Lignin Nanoparticles.					
35191679	7	18	theme	blocking	1360:1367	arg1	properties					1369:1378	ultraviolet blocking properties	1348:1378	ultraviolet blocking properties	1348:1378	What is more, the as-prepared LNP conferred strengthened thermal stability and ultraviolet blocking properties on the starch composite film.					
35191679	7	19	theme	as-prepared	1287:1297	arg1	LNP					1299:1301	the as-prepared LNP	1283:1301	the as-prepared LNP	1283:1301	What is more, the as-prepared LNP conferred strengthened thermal stability and ultraviolet blocking properties on the starch composite film.					
35191679	2	20	theme	"	278:278	arg1	LNP					301:303	LNP	301:303	LNP	301:303	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	2	20	theme	"	278:278	arg1	nanoparticle					287:298	a novel "core-shell" lignin nanoparticle	259:298	a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm	259:337	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	5	21	theme	starch/PVA	809:818	arg1	film					830:833	the starch/PVA composite film	805:833	the starch/PVA composite film	805:833	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	8	22	with	combination	1425:1435	arg1	LNPs					1462:1465	LNPs	1462:1465	LNPs	1462:1465	The structural combination of the polymer film with LNPs holds the promise for providing advanced functionalities to the composite material with wide applications.					
35191679	8	23	with	material	1541:1548	arg1	applications					1560:1571	wide applications	1555:1571	wide applications	1555:1571	The structural combination of the polymer film with LNPs holds the promise for providing advanced functionalities to the composite material with wide applications.					
35191679	2	24	theme	core-shell	268:277	arg1	LNP					301:303	LNP	301:303	LNP	301:303	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	2	24	theme	core-shell	268:277	arg1	nanoparticle					287:298	a novel "core-shell" lignin nanoparticle	259:298	a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm	259:337	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	1	25	theme	great	185:189	arg1	importance					191:200	great importance	185:200	great importance	185:200	Thehighly efficient utilization of lignin is of great importance for the development of the biorefinery industry.					
35191679	2	26	theme	acid	411:414	arg1	fractionation					416:428	formic acid fractionation	404:428	formic acid fractionation	404:428	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	8	27	theme	film	1452:1455	arg1	combination					1425:1435	The structural combination	1410:1435	The structural combination of the polymer film with LNPs	1410:1465	The structural combination of the polymer film with LNPs holds the promise for providing advanced functionalities to the composite material with wide applications.					
35191679	6	28	theme	structures	1059:1068	arg1	formation					1035:1043	the formation	1031:1043	the formation of some rough structures on the film surface	1031:1088	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	8	29	theme	structural	1414:1423	arg1	combination					1425:1435	The structural combination	1410:1435	The structural combination of the polymer film with LNPs	1410:1465	The structural combination of the polymer film with LNPs holds the promise for providing advanced functionalities to the composite material with wide applications.					
35191679	5	30	theme	film	830:833	arg1	value					796:800	the dynamic water contact angle value	764:800	the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs	764:863	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	2	31	theme	formic	404:409	arg1	fractionation					416:428	formic acid fractionation	404:428	formic acid fractionation	404:428	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	7	32	theme	strengthened	1313:1324	arg1	stability					1334:1342	strengthened thermal stability	1313:1342	strengthened thermal stability	1313:1342	What is more, the as-prepared LNP conferred strengthened thermal stability and ultraviolet blocking properties on the starch composite film.					
35191679	4	33	theme	hydrophobicity	670:683	arg1	performance					685:695	the hydrophobicity performance	666:695	the hydrophobicity performance of the synthesized films	666:720	The results showed that the hydrophobicity performance of the synthesized films was enhanced significantly.					
35191679	6	34	theme	water	1166:1170	arg1	molecules					1172:1180	the water molecules	1162:1180	the water molecules	1162:1180	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	5	35	theme	water	776:780	arg1	value					796:800	the dynamic water contact angle value	764:800	the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs	764:863	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	5	36	theme	dynamic	768:774	arg1	value					796:800	the dynamic water contact angle value	764:800	the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs	764:863	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	6	37	theme	enhanced	1225:1232	arg1	hydrophobicity					1234:1247	the enhanced hydrophobicity	1221:1247	the enhanced hydrophobicity of the starch film	1221:1266	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	6	38	theme	microscopy	1125:1134	arg1	images					1136:1141	the scanning electron microscopy images	1103:1141	the scanning electron microscopy images	1103:1141	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	6	38	theme	microscopy	1125:1134	arg1	related					1210:1216	related	1210:1216	related	1210:1216	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	5	39	theme	angle	790:794	arg1	value					796:800	the dynamic water contact angle value	764:800	the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs	764:863	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	5	40	theme	contact	782:788	arg1	value					796:800	the dynamic water contact angle value	764:800	the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs	764:863	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	3	41	theme	vital	492:496	arg1	functionalities					498:512	vital functionalities	492:512	vital functionalities	492:512	In an attempt to endow composite materials with vital functionalities, the LNP was added to the starch film and the starch/polyvinyl alcohol (PVA) or starch/polyethylene oxide (PEO) composite film.					
35191679	5	42	theme	contact	975:981	arg1	angle					983:987	an excellent water contact angle	956:987	an excellent water contact angle above 120°	956:998	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	5	43	dep	%	841:841	arg1	wt					844:845	wt	844:845	wt	844:845	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	5	44	theme	1	840:840	arg1	%					841:841	%	841:841	%	841:841	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	3	45	theme	starch/polyethylene	594:612	arg1	film					636:639	starch/polyethylene oxide (PEO) composite film	594:639	starch/polyethylene oxide (PEO) composite film	594:639	In an attempt to endow composite materials with vital functionalities, the LNP was added to the starch film and the starch/polyvinyl alcohol (PVA) or starch/polyethylene oxide (PEO) composite film.					
35191679	5	46	theme	starch/PEO	916:925	arg1	film					937:940	the starch/PEO composite film	912:940	the starch/PEO composite film	912:940	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	7	47	theme	thermal	1326:1332	arg1	stability					1334:1342	strengthened thermal stability	1313:1342	strengthened thermal stability	1313:1342	What is more, the as-prepared LNP conferred strengthened thermal stability and ultraviolet blocking properties on the starch composite film.					
35191679	0	48	theme	Enhanced	34:41	arg1	Hydrophobicity					43:56	Enhanced Hydrophobicity	34:56	Enhanced Hydrophobicity	34:56	Starch-Based Composite Films with Enhanced Hydrophobicity, Thermal Stability, and UV-Shielding Efficacy Induced by Lignin Nanoparticles.					
35191679	5	49	with	value	796:800	arg1	addition					848:855	1% (wt) addition	840:855	1% (wt) addition of LNPs	840:863	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	5	50	theme	composite	927:935	arg1	film					937:940	the starch/PEO composite film	912:940	the starch/PEO composite film	912:940	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	0	51	theme	Thermal	59:65	arg1	Stability					67:75	Thermal Stability	59:75	Thermal Stability	59:75	Starch-Based Composite Films with Enhanced Hydrophobicity, Thermal Stability, and UV-Shielding Efficacy Induced by Lignin Nanoparticles.					
35191679	2	52	attach	isolated	374:381	arg1	effluent					392:399	the effluent	388:399	the effluent of formic acid fractionation	388:428	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	2	52	attach	isolated	374:381	arg2	lignin					363:368	the lignin	359:368	the lignin	359:368	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	6	53	theme	scanning	1107:1114	arg1	microscopy					1125:1134	scanning electron microscopy	1107:1134	the scanning electron microscopy images	1103:1141	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	0	54	with	Films	23:27	arg1	Hydrophobicity					43:56	Enhanced Hydrophobicity	34:56	Enhanced Hydrophobicity	34:56	Starch-Based Composite Films with Enhanced Hydrophobicity, Thermal Stability, and UV-Shielding Efficacy Induced by Lignin Nanoparticles.					
35191679	0	54	with	Films	23:27	arg1	Stability					67:75	Thermal Stability	59:75	Thermal Stability	59:75	Starch-Based Composite Films with Enhanced Hydrophobicity, Thermal Stability, and UV-Shielding Efficacy Induced by Lignin Nanoparticles.					
35191679	0	54	with	Films	23:27	arg1	Efficacy					95:102	UV-Shielding Efficacy	82:102	UV-Shielding Efficacy	82:102	Starch-Based Composite Films with Enhanced Hydrophobicity, Thermal Stability, and UV-Shielding Efficacy Induced by Lignin Nanoparticles.					
35191679	2	55	theme	fractionation	416:428	arg1	effluent					392:399	the effluent	388:399	the effluent of formic acid fractionation	388:428	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	6	56	theme	film	1077:1080	arg1	surface					1082:1088	the film surface	1073:1088	the film surface	1073:1088	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	3	57	theme	PEO	621:623	arg1	film					636:639	starch/polyethylene oxide (PEO) composite film	594:639	starch/polyethylene oxide (PEO) composite film	594:639	In an attempt to endow composite materials with vital functionalities, the LNP was added to the starch film and the starch/polyvinyl alcohol (PVA) or starch/polyethylene oxide (PEO) composite film.					
35191679	3	58	theme	starch	540:545	arg1	film					547:550	the starch film	536:550	the starch film	536:550	In an attempt to endow composite materials with vital functionalities, the LNP was added to the starch film and the starch/polyvinyl alcohol (PVA) or starch/polyethylene oxide (PEO) composite film.					
35191679	6	59	from	formation	1035:1043	arg1	surface					1082:1088	the film surface	1073:1088	the film surface	1073:1088	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	8	60	theme	advanced	1499:1506	arg1	functionalities					1508:1522	advanced functionalities	1499:1522	advanced functionalities	1499:1522	The structural combination of the polymer film with LNPs holds the promise for providing advanced functionalities to the composite material with wide applications.					
35191679	0	61	theme	Starch-Based	0:11	arg1	Films					23:27	Starch-Based Composite Films	0:27	Starch-Based Composite Films with Enhanced Hydrophobicity, Thermal Stability, and UV-Shielding Efficacy	0:102	Starch-Based Composite Films with Enhanced Hydrophobicity, Thermal Stability, and UV-Shielding Efficacy Induced by Lignin Nanoparticles.					
35191679	7	62	theme	composite	1394:1402	arg1	film					1404:1407	the starch composite film	1383:1407	the starch composite film	1383:1407	What is more, the as-prepared LNP conferred strengthened thermal stability and ultraviolet blocking properties on the starch composite film.					
35191679	6	63	theme	electron	1116:1123	arg1	microscopy					1125:1134	scanning electron microscopy	1107:1134	the scanning electron microscopy images	1103:1141	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	6	64	theme	LNPs	1017:1020	arg1	addition					1005:1012	The addition	1001:1012	The addition of LNPs	1001:1020	The addition of LNPs promoted the formation of some rough structures on the film surface, as shown by the scanning electron microscopy images, which could repel the water molecules efficiently and are closely related to the enhanced hydrophobicity of the starch film.					
35191679	1	65	theme	biorefinery	229:239	arg1	industry					241:248	the biorefinery industry	225:248	the biorefinery industry	225:248	Thehighly efficient utilization of lignin is of great importance for the development of the biorefinery industry.					
35191679	8	66	theme	wide	1555:1558	arg1	applications					1560:1571	wide applications	1555:1571	wide applications	1555:1571	The structural combination of the polymer film with LNPs holds the promise for providing advanced functionalities to the composite material with wide applications.					
35191679	3	67	theme	oxide	614:618	arg1	film					636:639	starch/polyethylene oxide (PEO) composite film	594:639	starch/polyethylene oxide (PEO) composite film	594:639	In an attempt to endow composite materials with vital functionalities, the LNP was added to the starch film and the starch/polyvinyl alcohol (PVA) or starch/polyethylene oxide (PEO) composite film.					
35191679	2	68	theme	novel	261:265	arg1	LNP					301:303	LNP	301:303	LNP	301:303	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	2	68	theme	novel	261:265	arg1	nanoparticle					287:298	a novel "core-shell" lignin nanoparticle	259:298	a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm	259:337	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	1	69	theme	efficient	147:155	arg1	utilization					157:167	Thehighly efficient utilization	137:167	Thehighly efficient utilization of lignin	137:177	Thehighly efficient utilization of lignin is of great importance for the development of the biorefinery industry.					
35191679	1	70	theme	industry	241:248	arg1	development					210:220	the development	206:220	the development of the biorefinery industry	206:248	Thehighly efficient utilization of lignin is of great importance for the development of the biorefinery industry.					
35191679	5	71	theme	water	969:973	arg1	angle					983:987	an excellent water contact angle	956:987	an excellent water contact angle above 120°	956:998	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	8	72	theme	polymer	1444:1450	arg1	film					1452:1455	the polymer film	1440:1455	the polymer film	1440:1455	The structural combination of the polymer film with LNPs holds the promise for providing advanced functionalities to the composite material with wide applications.					
35191679	2	73	theme	nm	336:337	arg1	diameter					313:320	a diameter	311:320	a diameter of around 135 nm	311:337	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	5	74	theme	composite	820:828	arg1	film					830:833	the starch/PVA composite film	805:833	the starch/PVA composite film	805:833	Specifically, the dynamic water contact angle value of the starch/PVA composite film with 1% (wt) addition of LNPs could be maintained as high as 122° for 180 s; the starch/PEO composite film also achieved an excellent water contact angle above 120°.					
35191679	2	75	with	nanoparticle	287:298	arg1	diameter					313:320	a diameter	311:320	a diameter of around 135 nm	311:337	Herein, a novel "core-shell" lignin nanoparticle (LNP) with a diameter of around 135 nm was prepared, after the lignin was isolated from the effluent of formic acid fractionation via dialysis.					
35191679	3	76	theme	starch/polyvinyl	560:575	arg1	PVA					586:588	PVA	586:588	PVA	586:588	In an attempt to endow composite materials with vital functionalities, the LNP was added to the starch film and the starch/polyvinyl alcohol (PVA) or starch/polyethylene oxide (PEO) composite film.					
35191679	3	76	theme	starch/polyvinyl	560:575	arg1	alcohol					577:583	the starch/polyvinyl alcohol	556:583	the starch/polyvinyl alcohol (PVA)	556:589	In an attempt to endow composite materials with vital functionalities, the LNP was added to the starch film and the starch/polyvinyl alcohol (PVA) or starch/polyethylene oxide (PEO) composite film.					
35191679	0	77	theme	Composite	13:21	arg1	Films					23:27	Starch-Based Composite Films	0:27	Starch-Based Composite Films with Enhanced Hydrophobicity, Thermal Stability, and UV-Shielding Efficacy	0:102	Starch-Based Composite Films with Enhanced Hydrophobicity, Thermal Stability, and UV-Shielding Efficacy Induced by Lignin Nanoparticles.					
37245444	6	0	dep	abundance	1271:1279	arg1	the					1257:1259	the	1257:1259	the	1257:1259	The amendments altered the bacterial abundance and composition in soil.					
37245444	2	1	theme	shell	571:575	arg1	CSP					585:587	CSP	585:587	CSP	585:587	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	2	1	theme	shell	571:575	arg1	powder					577:582	crawfish shell powder	562:582	crawfish shell powder (CSP)	562:588	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	2	2	theme	shells	394:399	arg1	issue					376:380	the waste issue	366:380	the waste issue of crawfish shells	366:399	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	4	3	theme	CSP	843:845	arg1	Application					828:838	Application	828:838	Application of CSP and CSB	828:853	Application of CSP and CSB increased the soil available As concentration, while significant decreases were observed in the CT and CT-CSB treatments.					
37245444	11	4	theme	effective	2039:2047	arg1	CT-CSB					2009:2014	CT-CSB	2009:2014	CT-CSB	2009:2014	Overall, CT-CSB could be a potentially effective amendment for simultaneously immobilizing As and Pb and restoring soil ecological functions in contaminated arable soils.					
37245444	11	4	theme	effective	2039:2047	arg1	amendment					2049:2057	a potentially effective amendment	2025:2057	a potentially effective amendment for simultaneously immobilizing As and Pb and restoring soil ecological functions in contaminated arable soils	2025:2168	Overall, CT-CSB could be a potentially effective amendment for simultaneously immobilizing As and Pb and restoring soil ecological functions in contaminated arable soils.					
37245444	4	5	theme	CSB	851:853	arg1	Application					828:838	Application	828:838	Application of CSP and CSB	828:853	Application of CSP and CSB increased the soil available As concentration, while significant decreases were observed in the CT and CT-CSB treatments.					
37245444	5	6	theme	most	1220:1223	arg1	enzymes					1225:1231	most enzymes	1220:1231	most enzymes	1220:1231	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	9	7	theme	bacterial	1652:1660	arg1	structure					1672:1680	bacterial community structure	1652:1680	bacterial community structure	1652:1680	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	1	8	from	perspectives	342:353	arg1	concern					293:299	concern	293:299	concern	293:299	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements are of concern from both environmental and agricultural perspectives.					
37245444	1	9	theme	organic	209:215	arg1	biowaste					217:224	ever-increasing organic biowaste	193:224	ever-increasing organic biowaste	193:224	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements are of concern from both environmental and agricultural perspectives.					
37245444	10	10	theme	Partial	1759:1765	arg1	squares					1773:1779	Partial least squares	1759:1779	Partial least squares path modeling	1759:1793	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	2	11	theme	crawfish	532:539	arg1	biochar					547:553	crawfish shell biochar	532:553	crawfish shell biochar (CSB)	532:559	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	2	11	theme	crawfish	532:539	arg1	CSB					556:558	CSB	556:558	CSB	556:558	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	10	12	theme	strongest	1916:1924	arg1	predictor					1926:1934	the strongest predictor	1912:1934	the strongest predictor of As/Pb availability in soils	1912:1965	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	10	12	theme	strongest	1916:1924	arg1	property					1832:1839	soil chemical property	1818:1839	soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity)	1818:1906	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	1	13	theme	soil	237:240	arg1	contamination					242:254	arable soil contamination	230:254	arable soil contamination	230:254	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements are of concern from both environmental and agricultural perspectives.					
37245444	9	14	theme	bulk	1697:1700	arg1	density					1702:1708	bulk density	1697:1708	bulk density	1697:1708	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	3	15	theme	Pb	748:749	arg1	bioavailability					751:765	Pb bioavailability	748:765	Pb bioavailability	748:765	Results demonstrated that addition of all amendments decreased Pb bioavailability, with the greatest effect observed for the CT-CSB treatment.					
37245444	0	16	theme	co-contaminated	145:159	arg1	soil					161:164	an arsenic/lead co-contaminated soil	129:164	an arsenic/lead co-contaminated soil	129:164	Chitin and crawfish shell biochar composite decreased heavy metal bioavailability and shifted rhizosphere bacterial community in an arsenic/lead co-contaminated soil.					
37245444	5	17	theme	soil	1040:1043	arg1	cellobiohydrolase					1138:1154	cellobiohydrolase	1138:1154	cellobiohydrolase	1138:1154	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	5	17	theme	soil	1040:1043	arg1	α-glucosidase					1091:1103	α-glucosidase	1091:1103	α-glucosidase	1091:1103	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	5	17	theme	soil	1040:1043	arg1	activities					1052:1061	the soil enzyme activities	1036:1061	the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase	1036:1154	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	5	17	theme	soil	1040:1043	arg1	phosphatase					1078:1088	acid phosphatase	1073:1088	acid phosphatase	1073:1088	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	5	17	theme	soil	1040:1043	arg1	N-acetyl-β-glucosaminidase					1106:1131	N-acetyl-β-glucosaminidase	1106:1131	N-acetyl-β-glucosaminidase	1106:1131	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	7	18	theme	Chitinophagaceae	1370:1385	arg1	abundance					1387:1395	Chitinophagaceae abundance	1370:1395	Chitinophagaceae abundance	1370:1395	For instance, compared to the control, all treatments increased Chitinophagaceae abundance by 2.6-4.7%.					
37245444	2	19	theme	As/Pb	657:661	arg1	soil					679:682	As/Pb co-contaminated soil	657:682	As/Pb co-contaminated soil	657:682	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	3	20	theme	amendments	727:736	arg1	addition					711:718	addition	711:718	addition of all amendments	711:736	Results demonstrated that addition of all amendments decreased Pb bioavailability, with the greatest effect observed for the CT-CSB treatment.					
37245444	10	21	theme	squares	1773:1779	arg1	modeling					1786:1793	Partial least squares path modeling	1759:1793	Partial least squares path modeling	1759:1793	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	4	22	theme	available	874:882	arg1	concentration					887:899	the soil available As concentration	865:899	the soil available As concentration	865:899	Application of CSP and CSB increased the soil available As concentration, while significant decreases were observed in the CT and CT-CSB treatments.					
37245444	0	23	from	community	116:124	arg1	soil					161:164	an arsenic/lead co-contaminated soil	129:164	an arsenic/lead co-contaminated soil	129:164	Chitin and crawfish shell biochar composite decreased heavy metal bioavailability and shifted rhizosphere bacterial community in an arsenic/lead co-contaminated soil.					
37245444	10	24	theme	soil	1818:1821	arg1	predictor					1926:1934	the strongest predictor	1912:1934	the strongest predictor of As/Pb availability in soils	1912:1965	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	10	24	theme	soil	1818:1821	arg1	property					1832:1839	soil chemical property	1818:1839	soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity)	1818:1906	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	3	25	theme	CT-CSB	810:815	arg1	treatment					817:825	the CT-CSB treatment	806:825	the CT-CSB treatment	806:825	Results demonstrated that addition of all amendments decreased Pb bioavailability, with the greatest effect observed for the CT-CSB treatment.					
37245444	10	26	theme	amendment	1977:1985	arg1	application					1987:1997	amendment application	1977:1997	amendment application	1977:1997	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	0	27	theme	metal	60:64	arg1	bioavailability					66:80	heavy metal bioavailability	54:80	heavy metal bioavailability	54:80	Chitin and crawfish shell biochar composite decreased heavy metal bioavailability and shifted rhizosphere bacterial community in an arsenic/lead co-contaminated soil.					
37245444	11	28	theme	contaminated	2144:2155	arg1	soils					2164:2168	contaminated arable soils	2144:2168	contaminated arable soils	2144:2168	Overall, CT-CSB could be a potentially effective amendment for simultaneously immobilizing As and Pb and restoring soil ecological functions in contaminated arable soils.					
37245444	1	29	theme	Sustainable	167:177	arg1	management					179:188	Sustainable management	167:188	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements	167:284	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements are of concern from both environmental and agricultural perspectives.					
37245444	2	30	theme	pot	489:491	arg1	trial					493:497	a pot trial	487:497	a pot trial	487:497	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	4	31	located	observed	935:942	arg1	treatments					965:974	the CT and CT-CSB treatments	947:974	treatments	965:974	Application of CSP and CSB increased the soil available As concentration, while significant decreases were observed in the CT and CT-CSB treatments.					
37245444	4	31	located	observed	935:942	arg2	decreases					920:928	significant decreases	908:928	significant decreases	908:928	Application of CSP and CSB increased the soil available As concentration, while significant decreases were observed in the CT and CT-CSB treatments.					
37245444	9	32	theme	soils	1752:1756	arg1	availability					1736:1747	As/Pb availability	1730:1747	As/Pb availability	1730:1747	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	9	32	theme	soils	1752:1756	arg1	density					1702:1708	bulk density	1697:1708	bulk density	1697:1708	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	9	32	theme	soils	1752:1756	arg1	content					1717:1723	water content	1711:1723	water content	1711:1723	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	8	33	theme	%	1500:1500	arg1	increase					1502:1509	2.1% increase	1497:1509	2.1% increase of Comamonadaceae	1497:1527	The relative abundance of Comamonadaceae decreased by 1.6% in the CSB treatment, while 2.1% increase of Comamonadaceae was noted in the CT-CSB treatment.					
37245444	2	34	theme	CT-CSB	595:600	arg1	composite					602:610	CT-CSB composite	595:610	CT-CSB composite	595:610	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	0	35	theme	rhizosphere	94:104	arg1	community					116:124	rhizosphere bacterial community	94:124	rhizosphere bacterial community in an arsenic/lead co-contaminated soil	94:164	Chitin and crawfish shell biochar composite decreased heavy metal bioavailability and shifted rhizosphere bacterial community in an arsenic/lead co-contaminated soil.					
37245444	10	36	from	predictor	1926:1934	arg1	soils					1961:1965	soils	1961:1965	soils	1961:1965	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	2	37	theme	arsenic	443:449	arg1	threat					433:438	the threat	429:438	the threat of arsenic (As) and lead (Pb) to human health	429:484	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	5	38	theme	acid	1073:1076	arg1	phosphatase					1078:1088	acid phosphatase	1073:1088	acid phosphatase	1073:1088	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	11	39	theme	arable	2157:2162	arg1	soils					2164:2168	contaminated arable soils	2144:2168	contaminated arable soils	2144:2168	Overall, CT-CSB could be a potentially effective amendment for simultaneously immobilizing As and Pb and restoring soil ecological functions in contaminated arable soils.					
37245444	0	40	theme	Chitin	0:5	arg1	composite					34:42	Chitin and crawfish shell biochar composite	0:42	composite	34:42	Chitin and crawfish shell biochar composite decreased heavy metal bioavailability and shifted rhizosphere bacterial community in an arsenic/lead co-contaminated soil.					
37245444	1	41	theme	environmental	311:323	arg1	perspectives					342:353	both environmental and agricultural perspectives	306:353	both environmental and agricultural perspectives	306:353	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements are of concern from both environmental and agricultural perspectives.					
37245444	9	42	theme	Redundancy	1564:1573	arg1	analyses					1591:1598	Redundancy and correlation analyses	1564:1598	Redundancy and correlation analyses (at the family level)	1564:1620	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	0	43	theme	crawfish	11:18	arg1	composite					34:42	Chitin and crawfish shell biochar composite	0:42	composite	34:42	Chitin and crawfish shell biochar composite decreased heavy metal bioavailability and shifted rhizosphere bacterial community in an arsenic/lead co-contaminated soil.					
37245444	1	44	theme	agricultural	329:340	arg1	perspectives					342:353	both environmental and agricultural perspectives	306:353	both environmental and agricultural perspectives	306:353	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements are of concern from both environmental and agricultural perspectives.					
37245444	9	45	theme	correlation	1579:1589	arg1	analyses					1591:1598	Redundancy and correlation analyses	1564:1598	Redundancy and correlation analyses (at the family level)	1564:1620	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	8	46	theme	CT-CSB	1546:1551	arg1	treatment					1553:1561	the CT-CSB treatment	1542:1561	the CT-CSB treatment	1542:1561	The relative abundance of Comamonadaceae decreased by 1.6% in the CSB treatment, while 2.1% increase of Comamonadaceae was noted in the CT-CSB treatment.					
37245444	0	47	theme	biochar	26:32	arg1	composite					34:42	Chitin and crawfish shell biochar composite	0:42	composite	34:42	Chitin and crawfish shell biochar composite decreased heavy metal bioavailability and shifted rhizosphere bacterial community in an arsenic/lead co-contaminated soil.					
37245444	5	48	theme	CT	988:989	arg1	Meanwhile					977:985	Meanwhile	977:985	Meanwhile	977:985	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	5	48	theme	CT	988:989	arg1	addition					991:998	CT addition	988:998	CT addition	988:998	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	2	49	theme	human	473:477	arg1	health					479:484	human health	473:484	human health	473:484	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	8	50	theme	relative	1414:1421	arg1	abundance					1423:1431	The relative abundance	1410:1431	The relative abundance of Comamonadaceae	1410:1449	The relative abundance of Comamonadaceae decreased by 1.6% in the CSB treatment, while 2.1% increase of Comamonadaceae was noted in the CT-CSB treatment.					
37245444	2	51	theme	crawfish	385:392	arg1	shells					394:399	crawfish shells	385:399	crawfish shells	385:399	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	6	52	theme	bacterial	1261:1269	arg1	abundance					1271:1279	bacterial abundance	1261:1279	bacterial abundance	1261:1279	The amendments altered the bacterial abundance and composition in soil.					
37245444	10	53	theme	dissolved	1852:1860	arg1	carbon					1870:1875	dissolved organic carbon	1852:1875	dissolved organic carbon	1852:1875	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	6	54	from	composition	1285:1295	arg1	soil					1300:1303	soil	1300:1303	soil	1300:1303	The amendments altered the bacterial abundance and composition in soil.					
37245444	10	55	dep	pH	1848:1849	arg1	i.e.					1842:1845	i.e.	1842:1845	i.e.	1842:1845	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	2	56	dep	arsenic	443:449	arg1	As					452:453	As	452:453	As	452:453	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	2	57	theme	crawfish	562:569	arg1	CSP					585:587	CSP	585:587	CSP	585:587	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	2	57	theme	crawfish	562:569	arg1	powder					577:582	crawfish shell powder	562:582	crawfish shell powder (CSP)	562:588	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	11	58	theme	ecological	2120:2129	arg1	functions					2131:2139	soil ecological functions	2115:2139	soil ecological functions in contaminated arable soils	2115:2168	Overall, CT-CSB could be a potentially effective amendment for simultaneously immobilizing As and Pb and restoring soil ecological functions in contaminated arable soils.					
37245444	6	59	from	abundance	1271:1279	arg1	soil					1300:1303	soil	1300:1303	soil	1300:1303	The amendments altered the bacterial abundance and composition in soil.					
37245444	1	60	theme	ever-increasing	193:207	arg1	biowaste					217:224	ever-increasing organic biowaste	193:224	ever-increasing organic biowaste	193:224	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements are of concern from both environmental and agricultural perspectives.					
37245444	9	61	theme	community	1662:1670	arg1	structure					1672:1680	bacterial community structure	1652:1680	bacterial community structure	1652:1680	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	10	62	theme	exchange	1889:1896	arg1	capacity					1898:1905	cation exchange capacity	1882:1905	cation exchange capacity	1882:1905	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	10	63	theme	least	1767:1771	arg1	squares					1773:1779	Partial least squares	1759:1779	Partial least squares path modeling	1759:1793	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	1	64	theme	biowaste	217:224	arg1	management					179:188	Sustainable management	167:188	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements	167:284	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements are of concern from both environmental and agricultural perspectives.					
37245444	11	65	theme	soil	2115:2118	arg1	functions					2131:2139	soil ecological functions	2115:2139	soil ecological functions in contaminated arable soils	2115:2168	Overall, CT-CSB could be a potentially effective amendment for simultaneously immobilizing As and Pb and restoring soil ecological functions in contaminated arable soils.					
37245444	0	66	theme	arsenic/lead	132:143	arg1	soil					161:164	an arsenic/lead co-contaminated soil	129:164	an arsenic/lead co-contaminated soil	129:164	Chitin and crawfish shell biochar composite decreased heavy metal bioavailability and shifted rhizosphere bacterial community in an arsenic/lead co-contaminated soil.					
37245444	2	67	theme	shell	541:545	arg1	biochar					547:553	crawfish shell biochar	532:553	crawfish shell biochar (CSB)	532:559	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	2	67	theme	shell	541:545	arg1	CSB					556:558	CSB	556:558	CSB	556:558	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	1	68	theme	arable	230:235	arg1	contamination					242:254	arable soil contamination	230:254	arable soil contamination	230:254	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements are of concern from both environmental and agricultural perspectives.					
37245444	4	69	theme	As	884:885	arg1	concentration					887:899	the soil available As concentration	865:899	the soil available As concentration	865:899	Application of CSP and CSB increased the soil available As concentration, while significant decreases were observed in the CT and CT-CSB treatments.					
37245444	11	70	from	functions	2131:2139	arg1	soils					2164:2168	contaminated arable soils	2144:2168	contaminated arable soils	2144:2168	Overall, CT-CSB could be a potentially effective amendment for simultaneously immobilizing As and Pb and restoring soil ecological functions in contaminated arable soils.					
37245444	1	71	theme	contamination	242:254	arg1	management					179:188	Sustainable management	167:188	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements	167:284	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements are of concern from both environmental and agricultural perspectives.					
37245444	9	72	from	changes	1641:1647	arg1	structure					1672:1680	bacterial community structure	1652:1680	bacterial community structure	1652:1680	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	2	73	theme	co-contaminated	663:677	arg1	soil					679:682	As/Pb co-contaminated soil	657:682	As/Pb co-contaminated soil	657:682	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	5	74	theme	enzyme	1045:1050	arg1	cellobiohydrolase					1138:1154	cellobiohydrolase	1138:1154	cellobiohydrolase	1138:1154	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	5	74	theme	enzyme	1045:1050	arg1	α-glucosidase					1091:1103	α-glucosidase	1091:1103	α-glucosidase	1091:1103	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	5	74	theme	enzyme	1045:1050	arg1	activities					1052:1061	the soil enzyme activities	1036:1061	the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase	1036:1154	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	5	74	theme	enzyme	1045:1050	arg1	phosphatase					1078:1088	acid phosphatase	1073:1088	acid phosphatase	1073:1088	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	5	74	theme	enzyme	1045:1050	arg1	N-acetyl-β-glucosaminidase					1106:1131	N-acetyl-β-glucosaminidase	1106:1131	N-acetyl-β-glucosaminidase	1106:1131	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	10	75	theme	path	1781:1784	arg1	modeling					1786:1793	Partial least squares path modeling	1759:1793	Partial least squares path modeling	1759:1793	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	9	76	theme	water	1711:1715	arg1	content					1717:1723	water content	1711:1723	water content	1711:1723	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	8	77	theme	CSB	1476:1478	arg1	treatment					1480:1488	the CSB treatment	1472:1488	the CSB treatment	1472:1488	The relative abundance of Comamonadaceae decreased by 1.6% in the CSB treatment, while 2.1% increase of Comamonadaceae was noted in the CT-CSB treatment.					
37245444	10	78	theme	As/Pb	1939:1943	arg1	availability					1945:1956	As/Pb availability	1939:1956	As/Pb availability	1939:1956	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	10	79	theme	chemical	1823:1830	arg1	predictor					1926:1934	the strongest predictor	1912:1934	the strongest predictor of As/Pb availability in soils	1912:1965	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	10	79	theme	chemical	1823:1830	arg1	property					1832:1839	soil chemical property	1818:1839	soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity)	1818:1906	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	4	80	theme	soil	869:872	arg1	concentration					887:899	the soil available As concentration	865:899	the soil available As concentration	865:899	Application of CSP and CSB increased the soil available As concentration, while significant decreases were observed in the CT and CT-CSB treatments.					
37245444	3	81	theme	greatest	777:784	arg1	effect					786:791	the greatest effect	773:791	the greatest effect observed for the CT-CSB treatment	773:825	Results demonstrated that addition of all amendments decreased Pb bioavailability, with the greatest effect observed for the CT-CSB treatment.					
37245444	9	82	theme	As/Pb	1730:1734	arg1	availability					1736:1747	As/Pb availability	1730:1747	As/Pb availability	1730:1747	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	5	83	theme	enzymes	1225:1231	arg1	activities					1206:1215	the activities	1202:1215	the activities of most enzymes	1202:1231	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	10	84	theme	availability	1945:1956	arg1	predictor					1926:1934	the strongest predictor	1912:1934	the strongest predictor of As/Pb availability in soils	1912:1965	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	10	84	theme	availability	1945:1956	arg1	property					1832:1839	soil chemical property	1818:1839	soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity)	1818:1906	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	4	85	theme	CT	951:952	arg1	treatments					965:974	the CT and CT-CSB treatments	947:974	treatments	965:974	Application of CSP and CSB increased the soil available As concentration, while significant decreases were observed in the CT and CT-CSB treatments.					
37245444	0	86	theme	bacterial	106:114	arg1	community					116:124	rhizosphere bacterial community	94:124	rhizosphere bacterial community in an arsenic/lead co-contaminated soil	94:164	Chitin and crawfish shell biochar composite decreased heavy metal bioavailability and shifted rhizosphere bacterial community in an arsenic/lead co-contaminated soil.					
37245444	4	87	theme	significant	908:918	arg1	decreases					920:928	significant decreases	908:928	significant decreases	908:928	Application of CSP and CSB increased the soil available As concentration, while significant decreases were observed in the CT and CT-CSB treatments.					
37245444	8	88	theme	Comamonadaceae	1514:1527	arg1	increase					1502:1509	2.1% increase	1497:1509	2.1% increase of Comamonadaceae	1497:1527	The relative abundance of Comamonadaceae decreased by 1.6% in the CSB treatment, while 2.1% increase of Comamonadaceae was noted in the CT-CSB treatment.					
37245444	2	89	theme	remediation	629:639	arg1	efficiencies					641:652	their remediation efficiencies	623:652	their remediation efficiencies	623:652	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	9	90	from	level	1615:1619	arg1	analyses					1591:1598	Redundancy and correlation analyses	1564:1598	Redundancy and correlation analyses (at the family level)	1564:1620	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	0	91	theme	shell	20:24	arg1	composite					34:42	Chitin and crawfish shell biochar composite	0:42	composite	34:42	Chitin and crawfish shell biochar composite decreased heavy metal bioavailability and shifted rhizosphere bacterial community in an arsenic/lead co-contaminated soil.					
37245444	5	92	theme	CSB-containing	1165:1178	arg1	treatments					1180:1189	CSB-containing treatments	1165:1189	CSB-containing treatments	1165:1189	Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-β-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes.					
37245444	2	93	theme	lead	460:463	arg1	threat					433:438	the threat	429:438	the threat of arsenic (As) and lead (Pb) to human health	429:484	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	4	94	theme	CT-CSB	958:963	arg1	treatments					965:974	the CT and CT-CSB treatments	947:974	treatments	965:974	Application of CSP and CSB increased the soil available As concentration, while significant decreases were observed in the CT and CT-CSB treatments.					
37245444	0	95	theme	heavy	54:58	arg1	bioavailability					66:80	heavy metal bioavailability	54:80	heavy metal bioavailability	54:80	Chitin and crawfish shell biochar composite decreased heavy metal bioavailability and shifted rhizosphere bacterial community in an arsenic/lead co-contaminated soil.					
37245444	10	96	theme	organic	1862:1868	arg1	carbon					1870:1875	dissolved organic carbon	1852:1875	dissolved organic carbon	1852:1875	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	9	97	attach	linked	1687:1692	arg1	availability					1736:1747	As/Pb availability	1730:1747	As/Pb availability	1730:1747	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	9	97	attach	linked	1687:1692	arg1	density					1702:1708	bulk density	1697:1708	bulk density	1697:1708	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	9	97	attach	linked	1687:1692	arg1	content					1717:1723	water content	1711:1723	water content	1711:1723	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	9	97	attach	linked	1687:1692	arg2	changes					1641:1647	the changes	1637:1647	the changes in bacterial community structure	1637:1680	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	9	98	theme	family	1608:1613	arg1	level					1615:1619	the family level	1604:1619	the family level	1604:1619	Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils.					
37245444	2	99	theme	waste	370:374	arg1	issue					376:380	the waste issue	366:380	the waste issue of crawfish shells	366:399	To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil.					
37245444	1	100	theme	toxic	271:275	arg1	elements					277:284	potentially toxic elements	259:284	potentially toxic elements	259:284	Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements are of concern from both environmental and agricultural perspectives.					
37245444	10	101	theme	cation	1882:1887	arg1	capacity					1898:1905	cation exchange capacity	1882:1905	cation exchange capacity	1882:1905	Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application.					
37245444	8	102	theme	Comamonadaceae	1436:1449	arg1	abundance					1423:1431	The relative abundance	1410:1431	The relative abundance of Comamonadaceae	1410:1449	The relative abundance of Comamonadaceae decreased by 1.6% in the CSB treatment, while 2.1% increase of Comamonadaceae was noted in the CT-CSB treatment.					
36508691	6	0	theme	clinical	971:978	arg1	therapy					980:986	the clinical therapy	967:986	the clinical therapy of full-thickness wounds	967:1011	Overall, this multifunctional hydrogel could be an ideal wound dressing for the clinical therapy of full-thickness wounds.					
36508691	1	1	theme	healing	159:165	arg1	causes					138:143	Local causes	132:143	Local causes of slow wound healing	132:165	Local causes of slow wound healing include infection and wound hemorrhage.					
36508691	0	2	theme	Antioxidant	80:90	arg1	Properties					92:101	Antioxidant Properties	80:101	Antioxidant Properties	80:101	Carboxymethyl Chitosan/Tannic Acid Hydrogel with Antibacterial, Hemostasis, and Antioxidant Properties Promoting Skin Wound Repair.					
36508691	5	3	theme	wound	709:713	arg1	healing					715:721	the in vivo wound healing	697:721	the in vivo wound healing study	697:727	In the in vivo wound healing study, the results showed that the CMC-TA hydrogel could relieve inflammation and promote the recovery of skin incision, re-epithelialization, and collagen deposition.					
36508691	0	4	theme	Skin	113:116	arg1	Repair					124:129	Skin Wound Repair	113:129	Skin Wound Repair	113:129	Carboxymethyl Chitosan/Tannic Acid Hydrogel with Antibacterial, Hemostasis, and Antioxidant Properties Promoting Skin Wound Repair.					
36508691	5	5	theme	healing	715:721	arg1	study					723:727	the in vivo wound healing study	697:727	the in vivo wound healing study	697:727	In the in vivo wound healing study, the results showed that the CMC-TA hydrogel could relieve inflammation and promote the recovery of skin incision, re-epithelialization, and collagen deposition.					
36508691	2	6	theme	CMC-TA	299:304	arg1	hydrogels					317:325	carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	263:325	carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	263:325	Using sodium bicarbonate as a neutralizer, a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels solidify through hydrogen bonding in this study.					
36508691	6	7	theme	wound	948:952	arg1	hydrogel					921:928	this multifunctional hydrogel	900:928	this multifunctional hydrogel	900:928	Overall, this multifunctional hydrogel could be an ideal wound dressing for the clinical therapy of full-thickness wounds.					
36508691	6	7	theme	wound	948:952	arg1	dressing					954:961	an ideal wound dressing	939:961	an ideal wound dressing for the clinical therapy of full-thickness wounds	939:1011	Overall, this multifunctional hydrogel could be an ideal wound dressing for the clinical therapy of full-thickness wounds.					
36508691	2	8	from	bonding	353:359	arg1	study					369:373	this study	364:373	this study	364:373	Using sodium bicarbonate as a neutralizer, a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels solidify through hydrogen bonding in this study.					
36508691	2	9	theme	acid	293:296	arg1	hydrogels					317:325	carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	263:325	carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	263:325	Using sodium bicarbonate as a neutralizer, a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels solidify through hydrogen bonding in this study.					
36508691	0	10	theme	Wound	118:122	arg1	Repair					124:129	Skin Wound Repair	113:129	Skin Wound Repair	113:129	Carboxymethyl Chitosan/Tannic Acid Hydrogel with Antibacterial, Hemostasis, and Antioxidant Properties Promoting Skin Wound Repair.					
36508691	4	11	theme	in	536:537	arg1	tests					562:566	in vitro characterization tests	536:566	in vitro characterization tests	536:566	Following in vitro characterization tests, the CMC-TA hydrogel exhibited remarkable antibacterial and antioxidant properties, as well as quick hemostasis capabilities.					
36508691	2	12	theme	chitosan-tannic	277:291	arg1	hydrogels					317:325	carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	263:325	carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	263:325	Using sodium bicarbonate as a neutralizer, a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels solidify through hydrogen bonding in this study.					
36508691	0	13	theme	Chitosan/Tannic	14:28	arg1	Hydrogel					35:42	Carboxymethyl Chitosan/Tannic Acid Hydrogel	0:42	Carboxymethyl Chitosan/Tannic Acid Hydrogel with Antibacterial, Hemostasis, and Antioxidant Properties	0:101	Carboxymethyl Chitosan/Tannic Acid Hydrogel with Antibacterial, Hemostasis, and Antioxidant Properties Promoting Skin Wound Repair.					
36508691	4	14	dep	in	536:537	arg1	vitro					539:543	vitro	539:543	vitro	539:543	Following in vitro characterization tests, the CMC-TA hydrogel exhibited remarkable antibacterial and antioxidant properties, as well as quick hemostasis capabilities.					
36508691	1	15	theme	wound	189:193	arg1	hemorrhage					195:204	wound hemorrhage	189:204	wound hemorrhage	189:204	Local causes of slow wound healing include infection and wound hemorrhage.					
36508691	0	16	theme	Carboxymethyl	0:12	arg1	Hydrogel					35:42	Carboxymethyl Chitosan/Tannic Acid Hydrogel	0:42	Carboxymethyl Chitosan/Tannic Acid Hydrogel with Antibacterial, Hemostasis, and Antioxidant Properties	0:101	Carboxymethyl Chitosan/Tannic Acid Hydrogel with Antibacterial, Hemostasis, and Antioxidant Properties Promoting Skin Wound Repair.					
36508691	4	17	theme	antibacterial	610:622	arg1	properties					640:649	remarkable antibacterial and antioxidant properties	599:649	remarkable antibacterial and antioxidant properties	599:649	Following in vitro characterization tests, the CMC-TA hydrogel exhibited remarkable antibacterial and antioxidant properties, as well as quick hemostasis capabilities.					
36508691	5	18	theme	in	701:702	arg1	healing					715:721	the in vivo wound healing	697:721	the in vivo wound healing study	697:727	In the in vivo wound healing study, the results showed that the CMC-TA hydrogel could relieve inflammation and promote the recovery of skin incision, re-epithelialization, and collagen deposition.					
36508691	2	19	theme	hydrogels	317:325	arg1	variety					252:258	a variety	250:258	a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	250:325	Using sodium bicarbonate as a neutralizer, a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels solidify through hydrogen bonding in this study.					
36508691	2	19	theme	hydrogels	317:325	arg1	hydrogels					317:325	carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	263:325	carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	263:325	Using sodium bicarbonate as a neutralizer, a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels solidify through hydrogen bonding in this study.					
36508691	3	20	theme	TA	454:455	arg1	concentration					437:449	the concentration	433:449	the concentration of TA	433:455	The best-performing hydrogel was synthesized by altering the concentration of TA and exhibited remarkable mechanical properties and biocompatibility.					
36508691	3	21	theme	best-performing	380:394	arg1	hydrogel					396:403	The best-performing hydrogel	376:403	The best-performing hydrogel	376:403	The best-performing hydrogel was synthesized by altering the concentration of TA and exhibited remarkable mechanical properties and biocompatibility.					
36508691	4	22	theme	remarkable	599:608	arg1	properties					640:649	remarkable antibacterial and antioxidant properties	599:649	remarkable antibacterial and antioxidant properties	599:649	Following in vitro characterization tests, the CMC-TA hydrogel exhibited remarkable antibacterial and antioxidant properties, as well as quick hemostasis capabilities.					
36508691	2	23	theme	composite	307:315	arg1	hydrogels					317:325	carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	263:325	carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	263:325	Using sodium bicarbonate as a neutralizer, a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels solidify through hydrogen bonding in this study.					
36508691	0	24	theme	Acid	30:33	arg1	Hydrogel					35:42	Carboxymethyl Chitosan/Tannic Acid Hydrogel	0:42	Carboxymethyl Chitosan/Tannic Acid Hydrogel with Antibacterial, Hemostasis, and Antioxidant Properties	0:101	Carboxymethyl Chitosan/Tannic Acid Hydrogel with Antibacterial, Hemostasis, and Antioxidant Properties Promoting Skin Wound Repair.					
36508691	4	25	theme	antioxidant	628:638	arg1	properties					640:649	remarkable antibacterial and antioxidant properties	599:649	remarkable antibacterial and antioxidant properties	599:649	Following in vitro characterization tests, the CMC-TA hydrogel exhibited remarkable antibacterial and antioxidant properties, as well as quick hemostasis capabilities.					
36508691	5	26	theme	skin	829:832	arg1	incision					834:841	skin incision	829:841	skin incision	829:841	In the in vivo wound healing study, the results showed that the CMC-TA hydrogel could relieve inflammation and promote the recovery of skin incision, re-epithelialization, and collagen deposition.					
36508691	5	27	theme	CMC-TA	758:763	arg1	hydrogel					765:772	the CMC-TA hydrogel	754:772	the CMC-TA hydrogel	754:772	In the in vivo wound healing study, the results showed that the CMC-TA hydrogel could relieve inflammation and promote the recovery of skin incision, re-epithelialization, and collagen deposition.					
36508691	3	28	theme	remarkable	471:480	arg1	properties					493:502	remarkable mechanical properties	471:502	remarkable mechanical properties	471:502	The best-performing hydrogel was synthesized by altering the concentration of TA and exhibited remarkable mechanical properties and biocompatibility.					
36508691	5	29	theme	incision	834:841	arg1	recovery					817:824	the recovery	813:824	the recovery of skin incision, re-epithelialization, and collagen deposition	813:888	In the in vivo wound healing study, the results showed that the CMC-TA hydrogel could relieve inflammation and promote the recovery of skin incision, re-epithelialization, and collagen deposition.					
36508691	4	30	theme	CMC-TA	573:578	arg1	hydrogel					580:587	the CMC-TA hydrogel	569:587	the CMC-TA hydrogel	569:587	Following in vitro characterization tests, the CMC-TA hydrogel exhibited remarkable antibacterial and antioxidant properties, as well as quick hemostasis capabilities.					
36508691	5	31	dep	in	701:702	arg1	vivo					704:707	vivo	704:707	vivo	704:707	In the in vivo wound healing study, the results showed that the CMC-TA hydrogel could relieve inflammation and promote the recovery of skin incision, re-epithelialization, and collagen deposition.					
36508691	5	32	theme	collagen	870:877	arg1	deposition					879:888	collagen deposition	870:888	collagen deposition	870:888	In the in vivo wound healing study, the results showed that the CMC-TA hydrogel could relieve inflammation and promote the recovery of skin incision, re-epithelialization, and collagen deposition.					
36508691	0	33	with	Hydrogel	35:42	arg1	Antibacterial					49:61	Antibacterial	49:61	Antibacterial	49:61	Carboxymethyl Chitosan/Tannic Acid Hydrogel with Antibacterial, Hemostasis, and Antioxidant Properties Promoting Skin Wound Repair.					
36508691	0	33	with	Hydrogel	35:42	arg1	Properties					92:101	Antioxidant Properties	80:101	Antioxidant Properties	80:101	Carboxymethyl Chitosan/Tannic Acid Hydrogel with Antibacterial, Hemostasis, and Antioxidant Properties Promoting Skin Wound Repair.					
36508691	0	33	with	Hydrogel	35:42	arg1	Hemostasis					64:73	Hemostasis	64:73	Hemostasis	64:73	Carboxymethyl Chitosan/Tannic Acid Hydrogel with Antibacterial, Hemostasis, and Antioxidant Properties Promoting Skin Wound Repair.					
36508691	3	34	theme	mechanical	482:491	arg1	properties					493:502	remarkable mechanical properties	471:502	remarkable mechanical properties	471:502	The best-performing hydrogel was synthesized by altering the concentration of TA and exhibited remarkable mechanical properties and biocompatibility.					
36508691	6	35	theme	wounds	1006:1011	arg1	therapy					980:986	the clinical therapy	967:986	the clinical therapy of full-thickness wounds	967:1011	Overall, this multifunctional hydrogel could be an ideal wound dressing for the clinical therapy of full-thickness wounds.					
36508691	6	36	theme	multifunctional	905:919	arg1	hydrogel					921:928	this multifunctional hydrogel	900:928	this multifunctional hydrogel	900:928	Overall, this multifunctional hydrogel could be an ideal wound dressing for the clinical therapy of full-thickness wounds.					
36508691	6	36	theme	multifunctional	905:919	arg1	dressing					954:961	an ideal wound dressing	939:961	an ideal wound dressing for the clinical therapy of full-thickness wounds	939:1011	Overall, this multifunctional hydrogel could be an ideal wound dressing for the clinical therapy of full-thickness wounds.					
36508691	6	37	theme	ideal	942:946	arg1	hydrogel					921:928	this multifunctional hydrogel	900:928	this multifunctional hydrogel	900:928	Overall, this multifunctional hydrogel could be an ideal wound dressing for the clinical therapy of full-thickness wounds.					
36508691	6	37	theme	ideal	942:946	arg1	dressing					954:961	an ideal wound dressing	939:961	an ideal wound dressing for the clinical therapy of full-thickness wounds	939:1011	Overall, this multifunctional hydrogel could be an ideal wound dressing for the clinical therapy of full-thickness wounds.					
36508691	5	38	theme	re-epithelialization	844:863	arg1	recovery					817:824	the recovery	813:824	the recovery of skin incision, re-epithelialization, and collagen deposition	813:888	In the in vivo wound healing study, the results showed that the CMC-TA hydrogel could relieve inflammation and promote the recovery of skin incision, re-epithelialization, and collagen deposition.					
36508691	6	39	theme	full-thickness	991:1004	arg1	wounds					1006:1011	full-thickness wounds	991:1011	full-thickness wounds	991:1011	Overall, this multifunctional hydrogel could be an ideal wound dressing for the clinical therapy of full-thickness wounds.					
36508691	1	40	theme	Local	132:136	arg1	causes					138:143	Local causes	132:143	Local causes of slow wound healing	132:165	Local causes of slow wound healing include infection and wound hemorrhage.					
36508691	2	41	theme	sodium	213:218	arg1	neutralizer					237:247	a neutralizer	235:247	a neutralizer	235:247	Using sodium bicarbonate as a neutralizer, a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels solidify through hydrogen bonding in this study.					
36508691	2	41	theme	sodium	213:218	arg1	bicarbonate					220:230	sodium bicarbonate	213:230	sodium bicarbonate	213:230	Using sodium bicarbonate as a neutralizer, a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels solidify through hydrogen bonding in this study.					
36508691	2	42	theme	carboxymethyl	263:275	arg1	hydrogels					317:325	carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	263:325	carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels	263:325	Using sodium bicarbonate as a neutralizer, a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels solidify through hydrogen bonding in this study.					
36508691	2	43	theme	hydrogen	344:351	arg1	bonding					353:359	hydrogen bonding	344:359	hydrogen bonding in this study	344:373	Using sodium bicarbonate as a neutralizer, a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels solidify through hydrogen bonding in this study.					
36508691	4	44	theme	quick	663:667	arg1	capabilities					680:691	quick hemostasis capabilities	663:691	quick hemostasis capabilities	663:691	Following in vitro characterization tests, the CMC-TA hydrogel exhibited remarkable antibacterial and antioxidant properties, as well as quick hemostasis capabilities.					
36508691	1	45	theme	slow	148:151	arg1	healing					159:165	slow wound healing	148:165	slow wound healing	148:165	Local causes of slow wound healing include infection and wound hemorrhage.					
36508691	4	46	theme	characterization	545:560	arg1	tests					562:566	in vitro characterization tests	536:566	in vitro characterization tests	536:566	Following in vitro characterization tests, the CMC-TA hydrogel exhibited remarkable antibacterial and antioxidant properties, as well as quick hemostasis capabilities.					
36508691	5	47	theme	deposition	879:888	arg1	recovery					817:824	the recovery	813:824	the recovery of skin incision, re-epithelialization, and collagen deposition	813:888	In the in vivo wound healing study, the results showed that the CMC-TA hydrogel could relieve inflammation and promote the recovery of skin incision, re-epithelialization, and collagen deposition.					
36508691	1	48	theme	wound	153:157	arg1	healing					159:165	slow wound healing	148:165	slow wound healing	148:165	Local causes of slow wound healing include infection and wound hemorrhage.					
36508691	4	49	theme	hemostasis	669:678	arg1	capabilities					680:691	quick hemostasis capabilities	663:691	quick hemostasis capabilities	663:691	Following in vitro characterization tests, the CMC-TA hydrogel exhibited remarkable antibacterial and antioxidant properties, as well as quick hemostasis capabilities.					
36244062	8	0	theme	film	920:923	arg1	color					907:911	The color	903:911	The color of the film	903:923	The color of the film changed from redness to reddish-brown, and further to brownness, echoing the shrimp turned from fresh to spoiled.					
36244062	5	1	theme	incorporation	663:675	arg1	effect					649:654	The effect	645:654	The effect of the incorporation of WPPB on the properties of WPPP/MMT films	645:719	The effect of the incorporation of WPPB on the properties of WPPP/MMT films was investigated.					
36244062	9	2	theme	WPPP/MMT/WPPB-2	1050:1064	arg1	films					1076:1080	WPPP/MMT/WPPB-2 composite films	1050:1080	WPPP/MMT/WPPB-2 composite films	1050:1080	Therefore, WPPP/MMT/WPPB-2 composite films showed promise for the applications in monitoring the freshness of shrimp.					
36244062	9	3	theme	composite	1066:1074	arg1	films					1076:1080	WPPP/MMT/WPPB-2 composite films	1050:1080	WPPP/MMT/WPPB-2 composite films	1050:1080	Therefore, WPPP/MMT/WPPB-2 composite films showed promise for the applications in monitoring the freshness of shrimp.					
36244062	4	4	theme	film	639:642	arg1	sensitivity					620:630	the moisture sensitivity	607:630	the moisture sensitivity of the film	607:642	Furthermore, montmorillonite (MMT), a cheap and environmental-friendly silicate material, was introduced into film matrix as a filler to reduce the moisture sensitivity of the film.					
36244062	4	5	theme	film	573:576	arg1	matrix					578:583	film matrix	573:583	film matrix	573:583	Furthermore, montmorillonite (MMT), a cheap and environmental-friendly silicate material, was introduced into film matrix as a filler to reduce the moisture sensitivity of the film.					
36244062	3	6	theme	peel	339:342	arg1	WPPP					352:355	WPPP	352:355	WPPP	352:355	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	3	6	theme	peel	339:342	arg1	pectin					344:349	the white-fleshed pitaya peel pectin	314:349	the white-fleshed pitaya peel pectin (WPPP)	314:356	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	0	7	theme	novel	38:42	arg1	film					67:70	a novel active and intelligent film	36:70	a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus)	36:149	Development and characterization of a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus).					
36244062	4	8	theme	cheap	501:505	arg1	material					543:550	a cheap and environmental-friendly silicate material	499:550	a cheap and environmental-friendly silicate material	499:550	Furthermore, montmorillonite (MMT), a cheap and environmental-friendly silicate material, was introduced into film matrix as a filler to reduce the moisture sensitivity of the film.					
36244062	4	8	theme	cheap	501:505	arg1	montmorillonite					476:490	montmorillonite	476:490	montmorillonite (MMT)	476:496	Furthermore, montmorillonite (MMT), a cheap and environmental-friendly silicate material, was introduced into film matrix as a filler to reduce the moisture sensitivity of the film.					
36244062	2	9	theme	functional	252:261	arg1	film					263:266	a novel active and functional film	233:266	a novel active and functional film	233:266	was used to develop a novel active and functional film.					
36244062	3	10	theme	white-fleshed	318:330	arg1	WPPP					352:355	WPPP	352:355	WPPP	352:355	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	3	10	theme	white-fleshed	318:330	arg1	pectin					344:349	the white-fleshed pitaya peel pectin	314:349	the white-fleshed pitaya peel pectin (WPPP)	314:356	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	1	11	theme	present	159:165	arg1	study					167:171	the present study	155:171	the present study	155:171	In the present study, peel waste of pitaya (Hylocereus spp.)					
36244062	5	12	theme	WPPP/MMT	706:713	arg1	films					715:719	WPPP/MMT films	706:719	WPPP/MMT films	706:719	The effect of the incorporation of WPPB on the properties of WPPP/MMT films was investigated.					
36244062	2	13	theme	active	241:246	arg1	film					263:266	a novel active and functional film	233:266	a novel active and functional film	233:266	was used to develop a novel active and functional film.					
36244062	3	14	theme	white-fleshed	378:390	arg1	film					273:276	The film	269:276	The film	269:276	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	3	14	theme	white-fleshed	378:390	arg1	betacyanins					404:414	white-fleshed pitaya peel betacyanins	378:414	white-fleshed pitaya peel betacyanins	378:414	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	5	15	theme	films	715:719	arg1	properties					692:701	the properties	688:701	the properties of WPPP/MMT films	688:719	The effect of the incorporation of WPPB on the properties of WPPP/MMT films was investigated.					
36244062	0	16	theme	peel	109:112	arg1	waste					114:118	peel waste	109:118	peel waste of pitaya (Hylocereus undatus)	109:149	Development and characterization of a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus).					
36244062	6	17	theme	WPPP/MMT/WPPB	768:780	arg1	response					756:763	The colorimetric response	739:763	The colorimetric response of WPPP/MMT/WPPB to pH and ammonia	739:798	The colorimetric response of WPPP/MMT/WPPB to pH and ammonia was examined, respectively.					
36244062	2	18	theme	novel	235:239	arg1	film					263:266	a novel active and functional film	233:266	a novel active and functional film	233:266	was used to develop a novel active and functional film.					
36244062	3	19	theme	pitaya	392:397	arg1	film					273:276	The film	269:276	The film	269:276	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	3	19	theme	pitaya	392:397	arg1	betacyanins					404:414	white-fleshed pitaya peel betacyanins	378:414	white-fleshed pitaya peel betacyanins	378:414	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	3	20	theme	pectin	344:349	arg1	combination					299:309	a combination	297:309	a combination of the white-fleshed pitaya peel pectin (WPPP)	297:356	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	0	21	theme	pitaya	123:128	arg1	waste					114:118	peel waste	109:118	peel waste of pitaya (Hylocereus undatus)	109:149	Development and characterization of a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus).					
36244062	1	22	theme	peel	174:177	arg1	waste					179:183	peel waste	174:183	peel waste of pitaya	174:193	In the present study, peel waste of pitaya (Hylocereus spp.)					
36244062	5	23	theme	WPPB	680:683	arg1	incorporation					663:675	the incorporation	659:675	the incorporation of WPPB	659:683	The effect of the incorporation of WPPB on the properties of WPPP/MMT films was investigated.					
36244062	4	24	theme	silicate	534:541	arg1	material					543:550	a cheap and environmental-friendly silicate material	499:550	a cheap and environmental-friendly silicate material	499:550	Furthermore, montmorillonite (MMT), a cheap and environmental-friendly silicate material, was introduced into film matrix as a filler to reduce the moisture sensitivity of the film.					
36244062	4	24	theme	silicate	534:541	arg1	montmorillonite					476:490	montmorillonite	476:490	montmorillonite (MMT)	476:496	Furthermore, montmorillonite (MMT), a cheap and environmental-friendly silicate material, was introduced into film matrix as a filler to reduce the moisture sensitivity of the film.					
36244062	3	25	theme	pitaya	332:337	arg1	WPPP					352:355	WPPP	352:355	WPPP	352:355	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	3	25	theme	pitaya	332:337	arg1	pectin					344:349	the white-fleshed pitaya peel pectin	314:349	the white-fleshed pitaya peel pectin (WPPP)	314:356	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	6	26	theme	colorimetric	743:754	arg1	response					756:763	The colorimetric response	739:763	The colorimetric response of WPPP/MMT/WPPB to pH and ammonia	739:798	The colorimetric response of WPPP/MMT/WPPB to pH and ammonia was examined, respectively.					
36244062	4	27	theme	moisture	611:618	arg1	sensitivity					620:630	the moisture sensitivity	607:630	the moisture sensitivity of the film	607:642	Furthermore, montmorillonite (MMT), a cheap and environmental-friendly silicate material, was introduced into film matrix as a filler to reduce the moisture sensitivity of the film.					
36244062	0	28	theme	active	44:49	arg1	film					67:70	a novel active and intelligent film	36:70	a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus)	36:149	Development and characterization of a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus).					
36244062	8	29	dep	fresh	1021:1025	arg1	to					1027:1028	to	1027:1028	to	1027:1028	The color of the film changed from redness to reddish-brown, and further to brownness, echoing the shrimp turned from fresh to spoiled.					
36244062	8	29	dep	fresh	1021:1025	arg1	spoiled					1030:1036	spoiled	1030:1036	spoiled	1030:1036	The color of the film changed from redness to reddish-brown, and further to brownness, echoing the shrimp turned from fresh to spoiled.					
36244062	9	30	theme	shrimp	1149:1154	arg1	freshness					1136:1144	the freshness	1132:1144	the freshness of shrimp	1132:1154	Therefore, WPPP/MMT/WPPB-2 composite films showed promise for the applications in monitoring the freshness of shrimp.					
36244062	3	31	dep	biopolymer	363:372	arg1	a					361:361	a	361:361	a	361:361	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	3	32	theme	peel	399:402	arg1	film					273:276	The film	269:276	The film	269:276	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	3	32	theme	peel	399:402	arg1	betacyanins					404:414	white-fleshed pitaya peel betacyanins	378:414	white-fleshed pitaya peel betacyanins	378:414	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	5	33	from	effect	649:654	arg1	properties					692:701	the properties	688:701	the properties of WPPP/MMT films	688:719	The effect of the incorporation of WPPB on the properties of WPPP/MMT films was investigated.					
36244062	1	34	dep	waste	179:183	arg1	spp					207:209	spp	207:209	spp	207:209	In the present study, peel waste of pitaya (Hylocereus spp.)					
36244062	1	34	dep	waste	179:183	arg1	study					167:171	the present study	155:171	the present study	155:171	In the present study, peel waste of pitaya (Hylocereus spp.)					
36244062	1	35	theme	pitaya	188:193	arg1	waste					179:183	peel waste	174:183	peel waste of pitaya	174:193	In the present study, peel waste of pitaya (Hylocereus spp.)					
36244062	0	36	theme	film	67:70	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus).					
36244062	0	36	theme	film	67:70	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus).					
36244062	7	37	theme	shrimp	895:900	arg1	freshness					882:890	the freshness	878:890	the freshness of shrimp	878:900	Moreover, WPPP/MMT/WPPB-2 was employed to monitor the freshness of shrimp.					
36244062	0	38	dep	pitaya	123:128	arg1	undatus					142:148	Hylocereus undatus	131:148	Hylocereus undatus	131:148	Development and characterization of a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus).					
36244062	0	39	from	waste	114:118	arg1	betacyanins					92:102	betacyanins	92:102	betacyanins	92:102	Development and characterization of a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus).					
36244062	0	39	from	waste	114:118	arg1	pectin					81:86	pectin	81:86	pectin	81:86	Development and characterization of a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus).					
36244062	0	40	theme	intelligent	55:65	arg1	film					67:70	a novel active and intelligent film	36:70	a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus)	36:149	Development and characterization of a novel active and intelligent film based on pectin and betacyanins from peel waste of pitaya (Hylocereus undatus).					
36244062	3	41	theme	active	429:434	arg1	constituent					436:446	an active constituent	426:446	an active constituent	426:446	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	3	41	theme	active	429:434	arg1	film					273:276	The film	269:276	The film	269:276	The film was developed with a combination of the white-fleshed pitaya peel pectin (WPPP) as a biopolymer and white-fleshed pitaya peel betacyanins (WPPB) as an active constituent, respectively.					
36244062	4	42	theme	environmental-friendly	511:532	arg1	material					543:550	a cheap and environmental-friendly silicate material	499:550	a cheap and environmental-friendly silicate material	499:550	Furthermore, montmorillonite (MMT), a cheap and environmental-friendly silicate material, was introduced into film matrix as a filler to reduce the moisture sensitivity of the film.					
36244062	4	42	theme	environmental-friendly	511:532	arg1	montmorillonite					476:490	montmorillonite	476:490	montmorillonite (MMT)	476:496	Furthermore, montmorillonite (MMT), a cheap and environmental-friendly silicate material, was introduced into film matrix as a filler to reduce the moisture sensitivity of the film.					
36939183	0	0	theme	Cationic	87:94	arg1	Media					105:109	Aqueous and Cationic Micellar Media	75:109	Aqueous and Cationic Micellar Media	75:109	β-Cyclodextrin Stabilized Nanoceria for Hydrolytic Cleavage of Paraoxon in Aqueous and Cationic Micellar Media.					
36939183	4	1	theme	paraoxon	835:842	arg1	hydrolysis					821:830	the hydrolysis	817:830	the hydrolysis of paraoxon	817:842	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon is increased with increasing pH and the ζ-potential of β-CD@CeO2 NPs.					
36939183	2	2	theme	as-synthesized	342:355	arg1	NPs					367:369	the as-synthesized β-CD@CeO2 NPs	338:369	the as-synthesized β-CD@CeO2 NPs	338:369	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	5	3	from	mechanism	931:939	arg1	media					1004:1008	the aqueous and cationic micellar media	970:1008	the aqueous and cationic micellar media	970:1008	The kinetics and mechanism of hydrolysis of paraoxon in the aqueous and cationic micellar media have been discussed.					
36939183	3	4	theme	CeO2	663:666	arg1	NPs					668:670	β-CD@CeO2 NPs	658:670	β-CD@CeO2 NPs	658:670	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon were investigated.					
36939183	2	5	theme	electron	523:530	arg1	HRTEM					544:548	HRTEM	544:548	HRTEM	544:548	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	5	theme	electron	523:530	arg1	microscopy					532:541	high resolution transmission electron microscopy	494:541	high resolution transmission electron microscopy (HRTEM)	494:549	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	3	6	theme	NPs	668:670	arg1	ζ-potential					643:653	the ζ-potential	639:653	the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon	639:739	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon were investigated.					
36939183	2	7	theme	electron	580:587	arg1	microscopy					589:598	field emission scanning electron microscopy	556:598	field emission scanning electron microscopy (FESEM)	556:606	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	7	theme	electron	580:587	arg1	FESEM					601:605	FESEM	601:605	FESEM	601:605	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	5	8	theme	aqueous	974:980	arg1	media					1004:1008	the aqueous and cationic micellar media	970:1008	the aqueous and cationic micellar media	970:1008	The kinetics and mechanism of hydrolysis of paraoxon in the aqueous and cationic micellar media have been discussed.					
36939183	2	9	theme	surface	291:297	arg1	composition					299:309	surface composition	291:309	surface composition	291:309	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	0	10	theme	Micellar	96:103	arg1	Media					105:109	Aqueous and Cationic Micellar Media	75:109	Aqueous and Cationic Micellar Media	75:109	β-Cyclodextrin Stabilized Nanoceria for Hydrolytic Cleavage of Paraoxon in Aqueous and Cationic Micellar Media.					
36939183	2	11	theme	scanning	571:578	arg1	microscopy					589:598	field emission scanning electron microscopy	556:598	field emission scanning electron microscopy (FESEM)	556:606	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	11	theme	scanning	571:578	arg1	FESEM					601:605	FESEM	601:605	FESEM	601:605	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	12	theme	resolution	499:508	arg1	HRTEM					544:548	HRTEM	544:548	HRTEM	544:548	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	12	theme	resolution	499:508	arg1	microscopy					532:541	high resolution transmission electron microscopy	494:541	high resolution transmission electron microscopy (HRTEM)	494:549	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	13	theme	functional	273:282	arg1	group					284:288	surface functional group	265:288	surface functional group	265:288	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	13	theme	functional	273:282	arg1	NPs					367:369	the as-synthesized β-CD@CeO2 NPs	338:369	the as-synthesized β-CD@CeO2 NPs	338:369	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	14	theme	NPs	367:369	arg1	composition					299:309	surface composition	291:309	surface composition	291:309	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	14	theme	NPs	367:369	arg1	size					312:315	size	312:315	size	312:315	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	14	theme	NPs	367:369	arg1	group					284:288	surface functional group	265:288	surface functional group	265:288	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	14	theme	NPs	367:369	arg1	morphologies					322:333	morphologies	322:333	morphologies	322:333	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	14	theme	NPs	367:369	arg1	properties					253:262	The electronic properties	238:262	The electronic properties	238:262	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	14	theme	NPs	367:369	arg1	NPs					367:369	the as-synthesized β-CD@CeO2 NPs	338:369	the as-synthesized β-CD@CeO2 NPs	338:369	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	1	15	theme	β-CD	176:179	arg1	NPs					186:188	β-CD@CeO2 NPs	176:188	β-CD@CeO2 NPs	176:188	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	1	15	theme	β-CD	176:179	arg1	nanoparticles					161:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles	112:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs)	112:189	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	2	16	theme	high	494:497	arg1	HRTEM					544:548	HRTEM	544:548	HRTEM	544:548	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	16	theme	high	494:497	arg1	microscopy					532:541	high resolution transmission electron microscopy	494:541	high resolution transmission electron microscopy (HRTEM)	494:549	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	0	17	from	Cleavage	51:58	arg1	Media					105:109	Aqueous and Cationic Micellar Media	75:109	Aqueous and Cationic Micellar Media	75:109	β-Cyclodextrin Stabilized Nanoceria for Hydrolytic Cleavage of Paraoxon in Aqueous and Cationic Micellar Media.					
36939183	4	18	theme	pseudo-first-order	773:790	arg1	constant					797:804	The observed pseudo-first-order rate constant	760:804	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon	760:842	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon is increased with increasing pH and the ζ-potential of β-CD@CeO2 NPs.					
36939183	4	18	theme	pseudo-first-order	773:790	arg1	kobs					807:810	kobs	807:810	kobs	807:810	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon is increased with increasing pH and the ζ-potential of β-CD@CeO2 NPs.					
36939183	2	19	theme	surface	265:271	arg1	group					284:288	surface functional group	265:288	surface functional group	265:288	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	19	theme	surface	265:271	arg1	NPs					367:369	the as-synthesized β-CD@CeO2 NPs	338:369	the as-synthesized β-CD@CeO2 NPs	338:369	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	1	20	theme	@	180:180	arg1	NPs					186:188	β-CD@CeO2 NPs	176:188	β-CD@CeO2 NPs	176:188	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	1	20	theme	@	180:180	arg1	nanoparticles					161:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles	112:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs)	112:189	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	2	21	theme	high	436:439	arg1	HRXPS					486:490	HRXPS	486:490	HRXPS	486:490	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	21	theme	high	436:439	arg1	spectroscopy					472:483	high resolution X-ray photoelectron spectroscopy	436:483	high resolution X-ray photoelectron spectroscopy (HRXPS)	436:491	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	3	22	theme	activity	690:697	arg1	ζ-potential					643:653	the ζ-potential	639:653	the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon	639:739	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon were investigated.					
36939183	5	23	from	media	1004:1008	arg1	kinetics					918:925	kinetics	918:925	kinetics	918:925	The kinetics and mechanism of hydrolysis of paraoxon in the aqueous and cationic micellar media have been discussed.					
36939183	5	23	from	media	1004:1008	arg1	mechanism					931:939	mechanism	931:939	mechanism	931:939	The kinetics and mechanism of hydrolysis of paraoxon in the aqueous and cationic micellar media have been discussed.					
36939183	5	24	from	hydrolysis	944:953	arg1	media					1004:1008	the aqueous and cationic micellar media	970:1008	the aqueous and cationic micellar media	970:1008	The kinetics and mechanism of hydrolysis of paraoxon in the aqueous and cationic micellar media have been discussed.					
36939183	1	25	theme	CeO2	181:184	arg1	NPs					186:188	β-CD@CeO2 NPs	176:188	β-CD@CeO2 NPs	176:188	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	1	25	theme	CeO2	181:184	arg1	nanoparticles					161:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles	112:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs)	112:189	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	5	26	theme	cationic	986:993	arg1	media					1004:1008	the aqueous and cationic micellar media	970:1008	the aqueous and cationic micellar media	970:1008	The kinetics and mechanism of hydrolysis of paraoxon in the aqueous and cationic micellar media have been discussed.					
36939183	2	27	theme	CeO2	362:365	arg1	NPs					367:369	the as-synthesized β-CD@CeO2 NPs	338:369	the as-synthesized β-CD@CeO2 NPs	338:369	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	28	theme	β-CD	357:360	arg1	NPs					367:369	the as-synthesized β-CD@CeO2 NPs	338:369	the as-synthesized β-CD@CeO2 NPs	338:369	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	29	theme	FTIR	421:424	arg1	analysis					426:433	FTIR analysis	421:433	FTIR analysis	421:433	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	3	30	theme	NPs	706:708	arg1	activity					690:697	the catalytic activity	676:697	the catalytic activity of the NPs for the hydrolysis of paraoxon	676:739	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon were investigated.					
36939183	3	30	theme	NPs	706:708	arg1	NPs					668:670	β-CD@CeO2 NPs	658:670	β-CD@CeO2 NPs	658:670	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon were investigated.					
36939183	1	31	theme	Beta-cyclodextrin	112:128	arg1	NPs					186:188	β-CD@CeO2 NPs	176:188	β-CD@CeO2 NPs	176:188	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	1	31	theme	Beta-cyclodextrin	112:128	arg1	nanoparticles					161:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles	112:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs)	112:189	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	2	32	theme	@	361:361	arg1	NPs					367:369	the as-synthesized β-CD@CeO2 NPs	338:369	the as-synthesized β-CD@CeO2 NPs	338:369	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	5	33	theme	paraoxon	958:965	arg1	hydrolysis					944:953	hydrolysis	944:953	hydrolysis of paraoxon in the aqueous and cationic micellar media	944:1008	The kinetics and mechanism of hydrolysis of paraoxon in the aqueous and cationic micellar media have been discussed.					
36939183	4	34	theme	β-CD	899:902	arg1	NPs					909:911	β-CD@CeO2 NPs	899:911	β-CD@CeO2 NPs	899:911	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon is increased with increasing pH and the ζ-potential of β-CD@CeO2 NPs.					
36939183	2	35	theme	UV-visible	396:405	arg1	spectroscopy					407:418	UV-visible spectroscopy	396:418	UV-visible spectroscopy	396:418	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	0	36	theme	Hydrolytic	40:49	arg1	Cleavage					51:58	Hydrolytic Cleavage	40:58	Hydrolytic Cleavage of Paraoxon in Aqueous and Cationic Micellar Media	40:109	β-Cyclodextrin Stabilized Nanoceria for Hydrolytic Cleavage of Paraoxon in Aqueous and Cationic Micellar Media.					
36939183	2	37	theme	photoelectron	458:470	arg1	HRXPS					486:490	HRXPS	486:490	HRXPS	486:490	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	37	theme	photoelectron	458:470	arg1	spectroscopy					472:483	high resolution X-ray photoelectron spectroscopy	436:483	high resolution X-ray photoelectron spectroscopy (HRXPS)	436:491	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	3	38	theme	pH-dependent	613:624	arg1	variation					626:634	The pH-dependent variation	609:634	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon	609:739	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon were investigated.					
36939183	4	39	theme	rate	792:795	arg1	constant					797:804	The observed pseudo-first-order rate constant	760:804	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon	760:842	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon is increased with increasing pH and the ζ-potential of β-CD@CeO2 NPs.					
36939183	4	39	theme	rate	792:795	arg1	kobs					807:810	kobs	807:810	kobs	807:810	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon is increased with increasing pH and the ζ-potential of β-CD@CeO2 NPs.					
36939183	4	40	theme	NPs	909:911	arg1	pH					873:874	pH	873:874	pH	873:874	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon is increased with increasing pH and the ζ-potential of β-CD@CeO2 NPs.					
36939183	4	40	theme	NPs	909:911	arg1	ζ-potential					884:894	the ζ-potential	880:894	the ζ-potential of β-CD@CeO2 NPs	880:911	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon is increased with increasing pH and the ζ-potential of β-CD@CeO2 NPs.					
36939183	0	41	theme	Paraoxon	63:70	arg1	Cleavage					51:58	Hydrolytic Cleavage	40:58	Hydrolytic Cleavage of Paraoxon in Aqueous and Cationic Micellar Media	40:109	β-Cyclodextrin Stabilized Nanoceria for Hydrolytic Cleavage of Paraoxon in Aqueous and Cationic Micellar Media.					
36939183	4	42	theme	CeO2	904:907	arg1	NPs					909:911	β-CD@CeO2 NPs	899:911	β-CD@CeO2 NPs	899:911	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon is increased with increasing pH and the ζ-potential of β-CD@CeO2 NPs.					
36939183	2	43	theme	emission	562:569	arg1	microscopy					589:598	field emission scanning electron microscopy	556:598	field emission scanning electron microscopy (FESEM)	556:606	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	43	theme	emission	562:569	arg1	FESEM					601:605	FESEM	601:605	FESEM	601:605	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	4	44	theme	@	903:903	arg1	NPs					909:911	β-CD@CeO2 NPs	899:911	β-CD@CeO2 NPs	899:911	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon is increased with increasing pH and the ζ-potential of β-CD@CeO2 NPs.					
36939183	3	45	theme	catalytic	680:688	arg1	activity					690:697	the catalytic activity	676:697	the catalytic activity of the NPs for the hydrolysis of paraoxon	676:739	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon were investigated.					
36939183	2	46	theme	resolution	441:450	arg1	HRXPS					486:490	HRXPS	486:490	HRXPS	486:490	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	46	theme	resolution	441:450	arg1	spectroscopy					472:483	high resolution X-ray photoelectron spectroscopy	436:483	high resolution X-ray photoelectron spectroscopy (HRXPS)	436:491	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	47	theme	field	556:560	arg1	microscopy					589:598	field emission scanning electron microscopy	556:598	field emission scanning electron microscopy (FESEM)	556:606	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	47	theme	field	556:560	arg1	FESEM					601:605	FESEM	601:605	FESEM	601:605	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	3	48	theme	β-CD	658:661	arg1	NPs					668:670	β-CD@CeO2 NPs	658:670	β-CD@CeO2 NPs	658:670	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon were investigated.					
36939183	5	49	theme	micellar	995:1002	arg1	media					1004:1008	the aqueous and cationic micellar media	970:1008	the aqueous and cationic micellar media	970:1008	The kinetics and mechanism of hydrolysis of paraoxon in the aqueous and cationic micellar media have been discussed.					
36939183	3	50	theme	paraoxon	732:739	arg1	hydrolysis					718:727	the hydrolysis	714:727	the hydrolysis of paraoxon	714:739	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon were investigated.					
36939183	3	51	theme	@	662:662	arg1	NPs					668:670	β-CD@CeO2 NPs	658:670	β-CD@CeO2 NPs	658:670	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon were investigated.					
36939183	3	52	theme	ζ-potential	643:653	arg1	variation					626:634	The pH-dependent variation	609:634	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon	609:739	The pH-dependent variation of the ζ-potential of β-CD@CeO2 NPs and the catalytic activity of the NPs for the hydrolysis of paraoxon were investigated.					
36939183	5	53	theme	hydrolysis	944:953	arg1	kinetics					918:925	kinetics	918:925	kinetics	918:925	The kinetics and mechanism of hydrolysis of paraoxon in the aqueous and cationic micellar media have been discussed.					
36939183	5	53	theme	hydrolysis	944:953	arg1	mechanism					931:939	mechanism	931:939	mechanism	931:939	The kinetics and mechanism of hydrolysis of paraoxon in the aqueous and cationic micellar media have been discussed.					
36939183	1	54	theme	stabilized	137:146	arg1	NPs					186:188	β-CD@CeO2 NPs	176:188	β-CD@CeO2 NPs	176:188	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	1	54	theme	stabilized	137:146	arg1	nanoparticles					161:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles	112:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs)	112:189	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	1	55	theme	hydrothermal	218:229	arg1	route					231:235	a hydrothermal route	216:235	a hydrothermal route	216:235	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	0	56	theme	Aqueous	75:81	arg1	Media					105:109	Aqueous and Cationic Micellar Media	75:109	Aqueous and Cationic Micellar Media	75:109	β-Cyclodextrin Stabilized Nanoceria for Hydrolytic Cleavage of Paraoxon in Aqueous and Cationic Micellar Media.					
36939183	5	57	from	kinetics	918:925	arg1	media					1004:1008	the aqueous and cationic micellar media	970:1008	the aqueous and cationic micellar media	970:1008	The kinetics and mechanism of hydrolysis of paraoxon in the aqueous and cationic micellar media have been discussed.					
36939183	2	58	theme	electronic	242:251	arg1	properties					253:262	The electronic properties	238:262	The electronic properties	238:262	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	1	59	theme	cerium	148:153	arg1	NPs					186:188	β-CD@CeO2 NPs	176:188	β-CD@CeO2 NPs	176:188	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	1	59	theme	cerium	148:153	arg1	nanoparticles					161:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles	112:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs)	112:189	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	2	60	theme	X-ray	452:456	arg1	HRXPS					486:490	HRXPS	486:490	HRXPS	486:490	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	60	theme	X-ray	452:456	arg1	spectroscopy					472:483	high resolution X-ray photoelectron spectroscopy	436:483	high resolution X-ray photoelectron spectroscopy (HRXPS)	436:491	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	4	61	theme	observed	764:771	arg1	constant					797:804	The observed pseudo-first-order rate constant	760:804	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon	760:842	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon is increased with increasing pH and the ζ-potential of β-CD@CeO2 NPs.					
36939183	4	61	theme	observed	764:771	arg1	kobs					807:810	kobs	807:810	kobs	807:810	The observed pseudo-first-order rate constant (kobs) for the hydrolysis of paraoxon is increased with increasing pH and the ζ-potential of β-CD@CeO2 NPs.					
36939183	1	62	theme	oxide	155:159	arg1	NPs					186:188	β-CD@CeO2 NPs	176:188	β-CD@CeO2 NPs	176:188	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	1	62	theme	oxide	155:159	arg1	nanoparticles					161:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles	112:173	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs)	112:189	Beta-cyclodextrin (β-CD) stabilized cerium oxide nanoparticles (β-CD@CeO2 NPs) were synthesized through a hydrothermal route.					
36939183	2	63	theme	transmission	510:521	arg1	HRTEM					544:548	HRTEM	544:548	HRTEM	544:548	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36939183	2	63	theme	transmission	510:521	arg1	microscopy					532:541	high resolution transmission electron microscopy	494:541	high resolution transmission electron microscopy (HRTEM)	494:549	The electronic properties, surface functional group, surface composition, size, and morphologies of the as-synthesized β-CD@CeO2 NPs were characterized using UV-visible spectroscopy, FTIR analysis, high resolution X-ray photoelectron spectroscopy (HRXPS), high resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM).					
36737179	1	0	theme	biodegradable	201:213	arg1	packaging					227:235	biodegradable active food packaging	201:235	biodegradable active food packaging	201:235	With increasing environmental awareness and food safety concern worldwide, biodegradable active food packaging gained wide attention in recent years.					
36737179	5	1	from	dispersity	755:764	arg1	films					794:798	starch-based films	781:798	starch-based films	781:798	In this study, we developed a simple green method to improve the dispersity of graphene in starch-based films by modifying the graphene surfaces using mussel-inspired polydopamine and copper ions.					
36737179	4	2	theme	films	594:598	arg1	attributes					567:576	the functional attributes	552:576	the functional attributes of starch-based films	552:598	Carbon-based fillers can be used to enhance the functional attributes of starch-based films, but they are often difficult to incorporate because of their poor matrix dispersibility.					
36737179	7	3	theme	starch-based	1065:1076	arg1	films					1078:1082	the starch-based films	1061:1082	the starch-based films	1061:1082	The addition of the nanocomposites positively influenced the microstructure of the starch-based films, as well as impacting their mechanical, barrier, and thermal properties.					
36737179	1	4	theme	active	215:220	arg1	packaging					227:235	biodegradable active food packaging	201:235	biodegradable active food packaging	201:235	With increasing environmental awareness and food safety concern worldwide, biodegradable active food packaging gained wide attention in recent years.					
36737179	5	5	theme	starch-based	781:792	arg1	films					794:798	starch-based films	781:798	starch-based films	781:798	In this study, we developed a simple green method to improve the dispersity of graphene in starch-based films by modifying the graphene surfaces using mussel-inspired polydopamine and copper ions.					
36737179	4	6	theme	starch-based	581:592	arg1	films					594:598	starch-based films	581:598	starch-based films	581:598	Carbon-based fillers can be used to enhance the functional attributes of starch-based films, but they are often difficult to incorporate because of their poor matrix dispersibility.					
36737179	5	7	theme	simple	720:725	arg1	method					733:738	a simple green method	718:738	a simple green method to improve the dispersity of graphene in starch-based films by modifying the graphene surfaces using mussel-inspired polydopamine and copper ions	718:884	In this study, we developed a simple green method to improve the dispersity of graphene in starch-based films by modifying the graphene surfaces using mussel-inspired polydopamine and copper ions.					
36737179	7	8	theme	films	1078:1082	arg1	microstructure					1043:1056	the microstructure	1039:1056	the microstructure of the starch-based films	1039:1082	The addition of the nanocomposites positively influenced the microstructure of the starch-based films, as well as impacting their mechanical, barrier, and thermal properties.					
36737179	2	9	theme	biomaterials	330:341	arg1	biomaterials					330:341	the most potential biomaterials	311:341	the most potential biomaterials	311:341	Starch has been regarded as one of the most potential biomaterials to produce biodegradable films.					
36737179	2	9	theme	biomaterials	330:341	arg1	one					304:306	one	304:306	one	304:306	Starch has been regarded as one of the most potential biomaterials to produce biodegradable films.					
36737179	2	9	theme	biomaterials	330:341	arg1	Starch					276:281	Starch	276:281	Starch	276:281	Starch has been regarded as one of the most potential biomaterials to produce biodegradable films.					
36737179	4	10	theme	poor	662:665	arg1	dispersibility					674:687	their poor matrix dispersibility	656:687	their poor matrix dispersibility	656:687	Carbon-based fillers can be used to enhance the functional attributes of starch-based films, but they are often difficult to incorporate because of their poor matrix dispersibility.					
36737179	8	11	theme	promising	1265:1273	arg1	potential					1275:1283	promising potential	1265:1283	promising potential of the films acting as active food packaging	1265:1328	Additionally, the composite films exhibited antibacterial activity against food borne pathogens, suggesting promising potential of the films acting as active food packaging.					
36737179	5	12	theme	green	727:731	arg1	method					733:738	a simple green method	718:738	a simple green method to improve the dispersity of graphene in starch-based films by modifying the graphene surfaces using mussel-inspired polydopamine and copper ions	718:884	In this study, we developed a simple green method to improve the dispersity of graphene in starch-based films by modifying the graphene surfaces using mussel-inspired polydopamine and copper ions.					
36737179	2	13	theme	potential	320:328	arg1	biomaterials					330:341	the most potential biomaterials	311:341	the most potential biomaterials	311:341	Starch has been regarded as one of the most potential biomaterials to produce biodegradable films.					
36737179	8	14	theme	borne	1237:1241	arg1	pathogens					1243:1251	food borne pathogens	1232:1251	food borne pathogens	1232:1251	Additionally, the composite films exhibited antibacterial activity against food borne pathogens, suggesting promising potential of the films acting as active food packaging.					
36737179	3	15	theme	poor	395:398	arg1	performance					411:421	relatively poor functional performance	384:421	relatively poor functional performance of starch-based films	384:443	However, relatively poor functional performance of starch-based films severely limits their application as food packaging materials.					
36737179	2	16	theme	most	315:318	arg1	biomaterials					330:341	the most potential biomaterials	311:341	the most potential biomaterials	311:341	Starch has been regarded as one of the most potential biomaterials to produce biodegradable films.					
36737179	1	17	theme	food	222:225	arg1	packaging					227:235	biodegradable active food packaging	201:235	biodegradable active food packaging	201:235	With increasing environmental awareness and food safety concern worldwide, biodegradable active food packaging gained wide attention in recent years.					
36737179	3	18	theme	functional	400:409	arg1	performance					411:421	relatively poor functional performance	384:421	relatively poor functional performance of starch-based films	384:443	However, relatively poor functional performance of starch-based films severely limits their application as food packaging materials.					
36737179	4	19	theme	functional	556:565	arg1	attributes					567:576	the functional attributes	552:576	the functional attributes of starch-based films	552:598	Carbon-based fillers can be used to enhance the functional attributes of starch-based films, but they are often difficult to incorporate because of their poor matrix dispersibility.					
36737179	5	20	theme	mussel-inspired	841:855	arg1	polydopamine					857:868	mussel-inspired polydopamine and copper ions	841:884	polydopamine	857:868	In this study, we developed a simple green method to improve the dispersity of graphene in starch-based films by modifying the graphene surfaces using mussel-inspired polydopamine and copper ions.					
36737179	9	21	from	application	1484:1494	arg1	packaging					1518:1526	biodegradable food packaging	1499:1526	biodegradable food packaging	1499:1526	Overall, the method developed in this study has the potential for optimizing and endowing extra properties of starch-based films so as to increase their application in biodegradable food packaging.					
36737179	8	22	theme	antibacterial	1201:1213	arg1	activity					1215:1222	antibacterial activity	1201:1222	antibacterial activity against food borne pathogens	1201:1251	Additionally, the composite films exhibited antibacterial activity against food borne pathogens, suggesting promising potential of the films acting as active food packaging.					
36737179	3	23	theme	packaging	487:495	arg1	materials					497:505	food packaging materials	482:505	food packaging materials	482:505	However, relatively poor functional performance of starch-based films severely limits their application as food packaging materials.					
36737179	8	24	theme	films	1292:1296	arg1	potential					1275:1283	promising potential	1265:1283	promising potential of the films acting as active food packaging	1265:1328	Additionally, the composite films exhibited antibacterial activity against food borne pathogens, suggesting promising potential of the films acting as active food packaging.					
36737179	2	25	theme	biodegradable	354:366	arg1	films					368:372	biodegradable films	354:372	biodegradable films	354:372	Starch has been regarded as one of the most potential biomaterials to produce biodegradable films.					
36737179	8	26	theme	food	1315:1318	arg1	packaging					1320:1328	active food packaging	1308:1328	active food packaging	1308:1328	Additionally, the composite films exhibited antibacterial activity against food borne pathogens, suggesting promising potential of the films acting as active food packaging.					
36737179	1	27	theme	wide	244:247	arg1	attention					249:257	wide attention	244:257	wide attention in recent years	244:273	With increasing environmental awareness and food safety concern worldwide, biodegradable active food packaging gained wide attention in recent years.					
36737179	3	28	theme	starch-based	426:437	arg1	films					439:443	starch-based films	426:443	starch-based films	426:443	However, relatively poor functional performance of starch-based films severely limits their application as food packaging materials.					
36737179	9	29	theme	starch-based	1441:1452	arg1	films					1454:1458	starch-based films	1441:1458	starch-based films so as to increase their application in biodegradable food packaging	1441:1526	Overall, the method developed in this study has the potential for optimizing and endowing extra properties of starch-based films so as to increase their application in biodegradable food packaging.					
36737179	8	30	theme	composite	1175:1183	arg1	films					1185:1189	the composite films	1171:1189	the composite films	1171:1189	Additionally, the composite films exhibited antibacterial activity against food borne pathogens, suggesting promising potential of the films acting as active food packaging.					
36737179	1	31	theme	environmental	142:154	arg1	awareness					156:164	environmental awareness and food safety concern worldwide	142:198	awareness	156:164	With increasing environmental awareness and food safety concern worldwide, biodegradable active food packaging gained wide attention in recent years.					
36737179	3	32	theme	films	439:443	arg1	performance					411:421	relatively poor functional performance	384:421	relatively poor functional performance of starch-based films	384:443	However, relatively poor functional performance of starch-based films severely limits their application as food packaging materials.					
36737179	9	33	theme	films	1454:1458	arg1	properties					1427:1436	extra properties	1421:1436	extra properties of starch-based films so as to increase their application in biodegradable food packaging	1421:1526	Overall, the method developed in this study has the potential for optimizing and endowing extra properties of starch-based films so as to increase their application in biodegradable food packaging.					
36737179	0	34	theme	starch-based	43:54	arg1	films					56:60	active starch-based films	36:60	active starch-based films	36:60	Development and characterization of active starch-based films incorporating graphene/polydopamine/Cu2+ nanocomposite fillers.					
36737179	6	35	dep	showed	924:929	arg1	modified					972:979	modified	972:979	showed the surface of graphene was successfully modified	924:979	Spectroscopy and morphology analyses showed the surface of graphene was successfully modified.					
36737179	5	36	theme	graphene	769:776	arg1	dispersity					755:764	the dispersity	751:764	the dispersity of graphene in starch-based films	751:798	In this study, we developed a simple green method to improve the dispersity of graphene in starch-based films by modifying the graphene surfaces using mussel-inspired polydopamine and copper ions.					
36737179	6	37	theme	graphene	946:953	arg1	surface					935:941	the surface	931:941	the surface of graphene	931:953	Spectroscopy and morphology analyses showed the surface of graphene was successfully modified.					
36737179	6	38	dep	Spectroscopy	887:898	arg1	analyses					915:922	analyses	915:922	analyses	915:922	Spectroscopy and morphology analyses showed the surface of graphene was successfully modified.					
36737179	1	39	theme	recent	262:267	arg1	years					269:273	recent years	262:273	recent years	262:273	With increasing environmental awareness and food safety concern worldwide, biodegradable active food packaging gained wide attention in recent years.					
36737179	0	40	theme	active	36:41	arg1	films					56:60	active starch-based films	36:60	active starch-based films	36:60	Development and characterization of active starch-based films incorporating graphene/polydopamine/Cu2+ nanocomposite fillers.					
36737179	3	41	theme	food	482:485	arg1	materials					497:505	food packaging materials	482:505	food packaging materials	482:505	However, relatively poor functional performance of starch-based films severely limits their application as food packaging materials.					
36737179	8	42	theme	active	1308:1313	arg1	packaging					1320:1328	active food packaging	1308:1328	active food packaging	1308:1328	Additionally, the composite films exhibited antibacterial activity against food borne pathogens, suggesting promising potential of the films acting as active food packaging.					
36737179	7	43	theme	mechanical	1112:1121	arg1	properties					1145:1154	their mechanical, barrier, and thermal properties	1106:1154	their mechanical, barrier, and thermal properties	1106:1154	The addition of the nanocomposites positively influenced the microstructure of the starch-based films, as well as impacting their mechanical, barrier, and thermal properties.					
36737179	5	44	theme	copper	874:879	arg1	ions					881:884	mussel-inspired polydopamine and copper ions	841:884	ions	881:884	In this study, we developed a simple green method to improve the dispersity of graphene in starch-based films by modifying the graphene surfaces using mussel-inspired polydopamine and copper ions.					
36737179	4	45	used	used	536:539	arg2	fillers					521:527	Carbon-based fillers	508:527	Carbon-based fillers	508:527	Carbon-based fillers can be used to enhance the functional attributes of starch-based films, but they are often difficult to incorporate because of their poor matrix dispersibility.					
36737179	8	46	theme	food	1232:1235	arg1	pathogens					1243:1251	food borne pathogens	1232:1251	food borne pathogens	1232:1251	Additionally, the composite films exhibited antibacterial activity against food borne pathogens, suggesting promising potential of the films acting as active food packaging.					
36737179	0	47	theme	films	56:60	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of active starch-based films incorporating graphene/polydopamine/Cu2+ nanocomposite fillers.					
36737179	0	47	theme	films	56:60	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of active starch-based films incorporating graphene/polydopamine/Cu2+ nanocomposite fillers.					
36737179	7	48	theme	thermal	1137:1143	arg1	properties					1145:1154	their mechanical, barrier, and thermal properties	1106:1154	their mechanical, barrier, and thermal properties	1106:1154	The addition of the nanocomposites positively influenced the microstructure of the starch-based films, as well as impacting their mechanical, barrier, and thermal properties.					
36737179	1	49	from	attention	249:257	arg1	years					269:273	recent years	262:273	recent years	262:273	With increasing environmental awareness and food safety concern worldwide, biodegradable active food packaging gained wide attention in recent years.					
36737179	1	50	theme	food	170:173	arg1	worldwide					190:198	environmental awareness and food safety concern worldwide	142:198	worldwide	190:198	With increasing environmental awareness and food safety concern worldwide, biodegradable active food packaging gained wide attention in recent years.					
36737179	9	51	theme	extra	1421:1425	arg1	properties					1427:1436	extra properties	1421:1436	extra properties of starch-based films so as to increase their application in biodegradable food packaging	1421:1526	Overall, the method developed in this study has the potential for optimizing and endowing extra properties of starch-based films so as to increase their application in biodegradable food packaging.					
36737179	7	52	theme	barrier	1124:1130	arg1	properties					1145:1154	their mechanical, barrier, and thermal properties	1106:1154	their mechanical, barrier, and thermal properties	1106:1154	The addition of the nanocomposites positively influenced the microstructure of the starch-based films, as well as impacting their mechanical, barrier, and thermal properties.					
36737179	5	53	theme	graphene	817:824	arg1	surfaces					826:833	the graphene surfaces	813:833	the graphene surfaces using mussel-inspired polydopamine and copper ions	813:884	In this study, we developed a simple green method to improve the dispersity of graphene in starch-based films by modifying the graphene surfaces using mussel-inspired polydopamine and copper ions.					
36737179	1	54	theme	safety	175:180	arg1	worldwide					190:198	environmental awareness and food safety concern worldwide	142:198	worldwide	190:198	With increasing environmental awareness and food safety concern worldwide, biodegradable active food packaging gained wide attention in recent years.					
36737179	7	55	theme	nanocomposites	1002:1015	arg1	addition					986:993	The addition	982:993	The addition of the nanocomposites	982:1015	The addition of the nanocomposites positively influenced the microstructure of the starch-based films, as well as impacting their mechanical, barrier, and thermal properties.					
36737179	4	56	theme	Carbon-based	508:519	arg1	fillers					521:527	Carbon-based fillers	508:527	Carbon-based fillers	508:527	Carbon-based fillers can be used to enhance the functional attributes of starch-based films, but they are often difficult to incorporate because of their poor matrix dispersibility.					
36737179	1	57	theme	concern	182:188	arg1	worldwide					190:198	environmental awareness and food safety concern worldwide	142:198	worldwide	190:198	With increasing environmental awareness and food safety concern worldwide, biodegradable active food packaging gained wide attention in recent years.					
36737179	4	58	theme	matrix	667:672	arg1	dispersibility					674:687	their poor matrix dispersibility	656:687	their poor matrix dispersibility	656:687	Carbon-based fillers can be used to enhance the functional attributes of starch-based films, but they are often difficult to incorporate because of their poor matrix dispersibility.					
36737179	0	59	theme	nanocomposite	103:115	arg1	fillers					117:123	graphene/polydopamine/Cu2+ nanocomposite fillers	76:123	graphene/polydopamine/Cu2+ nanocomposite fillers	76:123	Development and characterization of active starch-based films incorporating graphene/polydopamine/Cu2+ nanocomposite fillers.					
36737179	9	60	theme	biodegradable	1499:1511	arg1	packaging					1518:1526	biodegradable food packaging	1499:1526	biodegradable food packaging	1499:1526	Overall, the method developed in this study has the potential for optimizing and endowing extra properties of starch-based films so as to increase their application in biodegradable food packaging.					
36737179	0	61	theme	graphene/polydopamine/Cu2+	76:101	arg1	fillers					117:123	graphene/polydopamine/Cu2+ nanocomposite fillers	76:123	graphene/polydopamine/Cu2+ nanocomposite fillers	76:123	Development and characterization of active starch-based films incorporating graphene/polydopamine/Cu2+ nanocomposite fillers.					
36737179	9	62	contain	has	1375:1377	arg1	method					1344:1349	the method	1340:1349	the method developed in this study	1340:1373	Overall, the method developed in this study has the potential for optimizing and endowing extra properties of starch-based films so as to increase their application in biodegradable food packaging.					
36737179	9	62	contain	has	1375:1377	arg2	potential					1383:1391	the potential	1379:1391	the potential for optimizing and endowing extra properties of starch-based films so as to increase their application in biodegradable food packaging	1379:1526	Overall, the method developed in this study has the potential for optimizing and endowing extra properties of starch-based films so as to increase their application in biodegradable food packaging.					
36737179	9	63	theme	food	1513:1516	arg1	packaging					1518:1526	biodegradable food packaging	1499:1526	biodegradable food packaging	1499:1526	Overall, the method developed in this study has the potential for optimizing and endowing extra properties of starch-based films so as to increase their application in biodegradable food packaging.					
36173727	4	0	dep	transform	799:807	arg1	infrared					809:816	infrared	809:816	transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric studies to understand the type of interactions between the composite beads	799:953	The prepared hydrogel beads were investigated using Fourier transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric studies to understand the type of interactions between the composite beads.					
36173727	1	1	dep	alginate	291:298	arg1	biopolymers					328:338	biopolymers	328:338	biopolymers	328:338	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	2	2	theme	gum	571:573	arg1	beads					588:592	sodium alginate and xanthan gum IPN hydrogel beads	543:592	sodium alginate and xanthan gum IPN hydrogel beads	543:592	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	7	3	theme	dissolution	1167:1177	arg1	studies					1179:1185	in vitro drug dissolution studies	1153:1185	in vitro drug dissolution studies	1153:1185	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	2	4	theme	hydrogel	449:456	arg1	beads					458:462	IPN hydrogel beads	445:462	IPN hydrogel beads	445:462	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	8	5	theme	zero-order	1414:1423	arg1	profile					1438:1444	zero-order drug release profile	1414:1444	zero-order drug release profile	1414:1444	This study demonstrated that inclusion of XAG has extended the drug release and able to achieve zero-order drug release profile.					
36173727	2	6	theme	xanthan	563:569	arg1	gum					571:573	xanthan gum	563:573	xanthan gum	563:573	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	2	7	theme	mass	621:624	arg1	ratios					626:631	different mass ratios	611:631	different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5)	611:673	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	7	8	contain	containing	1226:1235	arg1	preparation					1214:1224	SXF4 preparation	1209:1224	SXF4 preparation containing SAL and XAG polymers at 7:3 ratio	1209:1269	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	7	8	contain	containing	1226:1235	arg2	polymers					1249:1256	XAG polymers	1245:1256	XAG polymers	1245:1256	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	7	8	contain	containing	1226:1235	arg2	SAL					1237:1239	SAL	1237:1239	SAL	1237:1239	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	7	9	theme	drug	1162:1165	arg1	studies					1179:1185	in vitro drug dissolution studies	1153:1185	in vitro drug dissolution studies	1153:1185	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	2	10	theme	different	611:619	arg1	ratios					626:631	different mass ratios	611:631	different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5)	611:673	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	6	11	theme	beads	1119:1123	arg1	behavior					1089:1096	swelling behavior	1080:1096	swelling behavior	1080:1096	The particle size, drug entrapment efficiency, and swelling behavior of prepared hydrogel beads were also studied.					
36173727	6	11	theme	beads	1119:1123	arg1	size					1042:1045	The particle size	1029:1045	The particle size	1029:1045	The particle size, drug entrapment efficiency, and swelling behavior of prepared hydrogel beads were also studied.					
36173727	6	11	theme	beads	1119:1123	arg1	efficiency					1064:1073	drug entrapment efficiency	1048:1073	drug entrapment efficiency	1048:1073	The particle size, drug entrapment efficiency, and swelling behavior of prepared hydrogel beads were also studied.					
36173727	8	12	theme	drug	1381:1384	arg1	release					1386:1392	the drug release	1377:1392	the drug release	1377:1392	This study demonstrated that inclusion of XAG has extended the drug release and able to achieve zero-order drug release profile.					
36173727	1	13	theme	antidiabetic	250:261	arg1	glipizide					268:276	antidiabetic drug glipizide	250:276	antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers	250:338	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	0	14	theme	controlled	95:104	arg1	release					106:112	controlled release	95:112	controlled release of glipizide drug	95:130	Design, development and characterization of interpenetrating polymer network hydrogel bead for controlled release of glipizide drug.					
36173727	8	15	theme	release	1430:1436	arg1	profile					1438:1444	zero-order drug release profile	1414:1444	zero-order drug release profile	1414:1444	This study demonstrated that inclusion of XAG has extended the drug release and able to achieve zero-order drug release profile.					
36173727	2	16	theme	alginate	550:557	arg1	beads					588:592	sodium alginate and xanthan gum IPN hydrogel beads	543:592	sodium alginate and xanthan gum IPN hydrogel beads	543:592	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	6	17	theme	hydrogel	1110:1117	arg1	beads					1119:1123	prepared hydrogel beads	1101:1123	prepared hydrogel beads	1101:1123	The particle size, drug entrapment efficiency, and swelling behavior of prepared hydrogel beads were also studied.					
36173727	7	18	theme	in vitro	1153:1160	arg1	studies					1179:1185	in vitro drug dissolution studies	1153:1185	in vitro drug dissolution studies	1153:1185	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	1	19	theme	drug	263:266	arg1	glipizide					268:276	antidiabetic drug glipizide	250:276	antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers	250:338	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	0	20	theme	glipizide	117:125	arg1	drug					127:130	glipizide drug	117:130	glipizide drug	117:130	Design, development and characterization of interpenetrating polymer network hydrogel bead for controlled release of glipizide drug.					
36173727	8	21	theme	drug	1425:1428	arg1	profile					1438:1444	zero-order drug release profile	1414:1444	zero-order drug release profile	1414:1444	This study demonstrated that inclusion of XAG has extended the drug release and able to achieve zero-order drug release profile.					
36173727	2	22	theme	such	519:522	arg1	drug					537:540	such short acting drug	519:540	such short acting drug	519:540	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	6	23	theme	prepared	1101:1108	arg1	beads					1119:1123	prepared hydrogel beads	1101:1123	prepared hydrogel beads	1101:1123	The particle size, drug entrapment efficiency, and swelling behavior of prepared hydrogel beads were also studied.					
36173727	7	24	theme	XAG	1245:1247	arg1	polymers					1249:1256	XAG polymers	1245:1256	XAG polymers	1245:1256	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	1	25	theme	novel	157:161	arg1	bead					211:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	1	25	theme	novel	157:161	arg1	agent					412:416	cross-linking agent	398:416	cross-linking agent	398:416	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	4	26	theme	thermogravimetric	862:878	arg1	studies					880:886	thermogravimetric studies	862:886	thermogravimetric studies	862:886	The prepared hydrogel beads were investigated using Fourier transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric studies to understand the type of interactions between the composite beads.					
36173727	4	27	theme	interactions	914:925	arg1	type					906:909	the type	902:909	the type of interactions between the composite beads	902:953	The prepared hydrogel beads were investigated using Fourier transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric studies to understand the type of interactions between the composite beads.					
36173727	1	28	theme	interpenetrating	163:178	arg1	bead					211:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	1	28	theme	interpenetrating	163:178	arg1	agent					412:416	cross-linking agent	398:416	cross-linking agent	398:416	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	7	29	theme	%	1308:1308	arg1	release					1292:1298	extended drug release	1278:1298	extended drug release of 97.53% at 9 h	1278:1315	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	1	30	theme	ionotropic	343:352	arg1	technique					363:371	ionotropic gelation technique	343:371	ionotropic gelation technique with calcium chloride	343:393	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	2	31	theme	hydrogel	579:586	arg1	beads					588:592	sodium alginate and xanthan gum IPN hydrogel beads	543:592	sodium alginate and xanthan gum IPN hydrogel beads	543:592	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	2	32	theme	greater	472:478	arg1	benefits					480:487	greater benefits	472:487	greater benefits	472:487	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	7	33	from	9 h	1313:1315	arg1	release					1292:1298	extended drug release	1278:1298	extended drug release of 97.53% at 9 h	1278:1315	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	1	34	theme	polymer	180:186	arg1	bead					211:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	1	34	theme	polymer	180:186	arg1	agent					412:416	cross-linking agent	398:416	cross-linking agent	398:416	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	4	35	theme	composite	939:947	arg1	beads					949:953	the composite beads	935:953	the composite beads	935:953	The prepared hydrogel beads were investigated using Fourier transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric studies to understand the type of interactions between the composite beads.					
36173727	1	36	theme	glipizide	268:276	arg1	encapsulation					233:245	encapsulation	233:245	encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride	233:393	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	2	37	theme	IPN	575:577	arg1	beads					588:592	sodium alginate and xanthan gum IPN hydrogel beads	543:592	sodium alginate and xanthan gum IPN hydrogel beads	543:592	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	6	38	theme	swelling	1080:1087	arg1	behavior					1089:1096	swelling behavior	1080:1096	swelling behavior	1080:1096	The particle size, drug entrapment efficiency, and swelling behavior of prepared hydrogel beads were also studied.					
36173727	1	39	theme	network	188:194	arg1	bead					211:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	1	39	theme	network	188:194	arg1	agent					412:416	cross-linking agent	398:416	cross-linking agent	398:416	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	2	40	theme	short	524:528	arg1	drug					537:540	such short acting drug	519:540	such short acting drug	519:540	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	4	41	theme	hydrogel	752:759	arg1	beads					761:765	The prepared hydrogel beads	739:765	The prepared hydrogel beads	739:765	The prepared hydrogel beads were investigated using Fourier transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric studies to understand the type of interactions between the composite beads.					
36173727	1	42	theme	sodium	284:289	arg1	SAL					301:303	SAL	301:303	SAL	301:303	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	1	42	theme	sodium	284:289	arg1	alginate					291:298	sodium alginate	284:298	sodium alginate (SAL)	284:304	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	0	43	theme	drug	127:130	arg1	release					106:112	controlled release	95:112	controlled release of glipizide drug	95:130	Design, development and characterization of interpenetrating polymer network hydrogel bead for controlled release of glipizide drug.					
36173727	1	44	theme	gelation	354:361	arg1	technique					363:371	ionotropic gelation technique	343:371	ionotropic gelation technique with calcium chloride	343:393	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	2	45	theme	fact	435:438	arg1	light					422:426	light	422:426	light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug	422:540	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	1	46	theme	IPN	197:199	arg1	bead					211:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	1	46	theme	IPN	197:199	arg1	agent					412:416	cross-linking agent	398:416	cross-linking agent	398:416	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	2	47	theme	IPN	445:447	arg1	beads					458:462	IPN hydrogel beads	445:462	IPN hydrogel beads	445:462	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	7	48	theme	SXF4	1209:1212	arg1	preparation					1214:1224	SXF4 preparation	1209:1224	SXF4 preparation containing SAL and XAG polymers at 7:3 ratio	1209:1269	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	5	49	theme	scanning	999:1006	arg1	microscopy					1017:1026	scanning electron microscopy	999:1026	scanning electron microscopy	999:1026	Surface morphology changes were studied by scanning electron microscopy.					
36173727	1	50	with	technique	363:371	arg1	chloride					386:393	calcium chloride	378:393	calcium chloride	378:393	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	4	51	theme	powder	838:843	arg1	diffraction					845:855	X-ray powder diffraction	832:855	X-ray powder diffraction	832:855	The prepared hydrogel beads were investigated using Fourier transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric studies to understand the type of interactions between the composite beads.					
36173727	5	52	theme	electron	1008:1015	arg1	microscopy					1017:1026	scanning electron microscopy	999:1026	scanning electron microscopy	999:1026	Surface morphology changes were studied by scanning electron microscopy.					
36173727	2	53	theme	=	642:642	arg1	10:0					644:647	XAG = 10:0	638:647	XAG = 10:0	638:647	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	8	54	theme	XAG	1360:1362	arg1	inclusion					1347:1355	inclusion	1347:1355	inclusion of XAG	1347:1362	This study demonstrated that inclusion of XAG has extended the drug release and able to achieve zero-order drug release profile.					
36173727	1	55	theme	hydrogel	202:209	arg1	bead					211:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	1	55	theme	hydrogel	202:209	arg1	agent					412:416	cross-linking agent	398:416	cross-linking agent	398:416	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	0	56	theme	polymer	61:67	arg1	bead					86:89	polymer network hydrogel bead	61:89	polymer network hydrogel bead for controlled release of glipizide drug	61:130	Design, development and characterization of interpenetrating polymer network hydrogel bead for controlled release of glipizide drug.					
36173727	2	57	theme	sodium	543:548	arg1	alginate					550:557	sodium alginate	543:557	sodium alginate	543:557	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	1	58	theme	calcium	378:384	arg1	chloride					386:393	calcium chloride	378:393	calcium chloride	378:393	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	4	59	theme	prepared	743:750	arg1	beads					761:765	The prepared hydrogel beads	739:765	The prepared hydrogel beads	739:765	The prepared hydrogel beads were investigated using Fourier transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric studies to understand the type of interactions between the composite beads.					
36173727	4	60	dep	Fourier	791:797	arg1	transform					799:807	transform	799:807	transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric studies to understand the type of interactions between the composite beads	799:953	The prepared hydrogel beads were investigated using Fourier transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric studies to understand the type of interactions between the composite beads.					
36173727	4	61	theme	X-ray	832:836	arg1	diffraction					845:855	X-ray powder diffraction	832:855	X-ray powder diffraction	832:855	The prepared hydrogel beads were investigated using Fourier transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric studies to understand the type of interactions between the composite beads.					
36173727	5	62	theme	morphology	964:973	arg1	changes					975:981	Surface morphology changes	956:981	Surface morphology changes	956:981	Surface morphology changes were studied by scanning electron microscopy.					
36173727	5	63	theme	Surface	956:962	arg1	changes					975:981	Surface morphology changes	956:981	Surface morphology changes	956:981	Surface morphology changes were studied by scanning electron microscopy.					
36173727	2	64	contain	possess	464:470	arg1	beads					458:462	IPN hydrogel beads	445:462	IPN hydrogel beads	445:462	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	2	64	contain	possess	464:470	arg2	benefits					480:487	greater benefits	472:487	greater benefits	472:487	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	6	65	theme	entrapment	1053:1062	arg1	efficiency					1064:1073	drug entrapment efficiency	1048:1073	drug entrapment efficiency	1048:1073	The particle size, drug entrapment efficiency, and swelling behavior of prepared hydrogel beads were also studied.					
36173727	2	66	theme	drug	537:540	arg1	release					508:514	the release	504:514	the release of such short acting drug	504:540	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	7	67	theme	extended	1278:1285	arg1	release					1292:1298	extended drug release	1278:1298	extended drug release of 97.53% at 9 h	1278:1315	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	2	68	theme	XAG	638:640	arg1	10:0					644:647	XAG = 10:0	638:647	XAG = 10:0	638:647	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	6	69	theme	drug	1048:1051	arg1	efficiency					1064:1073	drug entrapment efficiency	1048:1073	drug entrapment efficiency	1048:1073	The particle size, drug entrapment efficiency, and swelling behavior of prepared hydrogel beads were also studied.					
36173727	2	70	dep	ratios	626:631	arg1	8:2					655:657	8:2	655:657	8:2	655:657	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	2	70	dep	ratios	626:631	arg1	5:5					670:672	5:5	670:672	5:5	670:672	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	2	70	dep	ratios	626:631	arg1	7:3					660:662	7:3	660:662	7:3	660:662	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	2	70	dep	ratios	626:631	arg1	6:4					665:667	6:4	665:667	6:4	665:667	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	2	70	dep	ratios	626:631	arg1	SAL					634:636	SAL	634:636	SAL	634:636	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	2	70	dep	ratios	626:631	arg1	9:1					650:652	9:1	650:652	9:1	650:652	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	3	71	theme	IPN	699:701	arg1	beads					712:716	drug-loaded IPN hydrogel beads	687:716	drug-loaded IPN hydrogel beads	687:716	Similarly, drug-loaded IPN hydrogel beads were also developed.					
36173727	2	72	theme	acting	530:535	arg1	drug					537:540	such short acting drug	519:540	such short acting drug	519:540	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	1	73	theme	cross-linking	398:410	arg1	bead					211:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	a novel interpenetrating polymer network (IPN) hydrogel bead	155:214	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	1	73	theme	cross-linking	398:410	arg1	agent					412:416	cross-linking agent	398:416	cross-linking agent	398:416	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	7	74	theme	drug	1287:1290	arg1	release					1292:1298	extended drug release	1278:1298	extended drug release of 97.53% at 9 h	1278:1315	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	7	75	theme	7:3	1261:1263	arg1	ratio					1265:1269	7:3 ratio	1261:1269	7:3 ratio	1261:1269	Based on in vitro drug dissolution studies, it was observed that SXF4 preparation containing SAL and XAG polymers at 7:3 ratio showed extended drug release of 97.53% at 9 h.					
36173727	3	76	theme	hydrogel	703:710	arg1	beads					712:716	drug-loaded IPN hydrogel beads	687:716	drug-loaded IPN hydrogel beads	687:716	Similarly, drug-loaded IPN hydrogel beads were also developed.					
36173727	0	77	theme	hydrogel	77:84	arg1	bead					86:89	polymer network hydrogel bead	61:89	polymer network hydrogel bead for controlled release of glipizide drug	61:130	Design, development and characterization of interpenetrating polymer network hydrogel bead for controlled release of glipizide drug.					
36173727	1	78	theme	current	140:146	arg1	study					148:152	the current study	136:152	the current study	136:152	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	2	79	dep	SAL	634:636	arg1	10:0					644:647	XAG = 10:0	638:647	XAG = 10:0	638:647	In light of the fact that IPN hydrogel beads possess greater benefits in controlling the release of such short acting drug, sodium alginate and xanthan gum IPN hydrogel beads were prepared at different mass ratios (SAL:XAG = 10:0, 9:1, 8:2, 7:3, 6:4, 5:5).					
36173727	0	80	theme	network	69:75	arg1	bead					86:89	polymer network hydrogel bead	61:89	polymer network hydrogel bead for controlled release of glipizide drug	61:130	Design, development and characterization of interpenetrating polymer network hydrogel bead for controlled release of glipizide drug.					
36173727	1	81	theme	xanthan	310:316	arg1	XAG					323:325	XAG	323:325	XAG	323:325	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	1	81	theme	xanthan	310:316	arg1	gum					318:320	xanthan gum	310:320	xanthan gum (XAG)	310:326	In the current study, a novel interpenetrating polymer network (IPN) hydrogel bead was developed by encapsulation of antidiabetic drug glipizide using sodium alginate (SAL) and xanthan gum (XAG) biopolymers by ionotropic gelation technique with calcium chloride as cross-linking agent.					
36173727	6	82	theme	particle	1033:1040	arg1	size					1042:1045	The particle size	1029:1045	The particle size	1029:1045	The particle size, drug entrapment efficiency, and swelling behavior of prepared hydrogel beads were also studied.					
36173727	3	83	theme	drug-loaded	687:697	arg1	beads					712:716	drug-loaded IPN hydrogel beads	687:716	drug-loaded IPN hydrogel beads	687:716	Similarly, drug-loaded IPN hydrogel beads were also developed.					
37148946	0	0	theme	nanoflowers	80:90	arg1	matrix					109:114	ZnO nanoflowers impeded chitosan matrix	76:114	ZnO nanoflowers impeded chitosan matrix	76:114	Biological characterization of microwave based synthesized ZnO and Ce doped ZnO nanoflowers impeded chitosan matrix with enhanced antioxidant and anti-diabetic properties.					
37148946	6	1	theme	crude	1398:1402	arg1	%					1442:1442	96.8 ± 1.16 %	1430:1442	96.8 ± 1.16 %	1430:1442	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	1	theme	crude	1398:1402	arg1	α-glucosidase					1415:1427	crude intestinal α-glucosidase	1398:1427	crude intestinal α-glucosidase (96.8 ± 1.16 %)	1398:1443	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	2	theme	crude	1323:1327	arg1	α-amylase					1329:1337	crude α-amylase	1323:1337	crude α-amylase (88.7 ± 1.82 %)	1323:1353	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	2	theme	crude	1323:1327	arg1	%					1352:1352	88.7 ± 1.82 %	1340:1352	88.7 ± 1.82 %	1340:1352	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	8	3	dep	potential	1681:1689	arg1	antidiabetic					1691:1702	antidiabetic	1691:1702	antidiabetic	1691:1702	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	0	4	theme	ZnO	76:78	arg1	matrix					109:114	ZnO nanoflowers impeded chitosan matrix	76:114	ZnO nanoflowers impeded chitosan matrix	76:114	Biological characterization of microwave based synthesized ZnO and Ce doped ZnO nanoflowers impeded chitosan matrix with enhanced antioxidant and anti-diabetic properties.					
37148946	1	5	theme	chitosan	176:183	arg1	substrate					206:214	a substrate	204:214	a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH)	204:284	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	1	5	theme	chitosan	176:183	arg1	matrix					185:190	The chitosan matrix	172:190	The chitosan matrix	172:190	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	8	6	theme	chemical	1805:1812	arg1	drug					1814:1817	the commonly used chemical drug	1787:1817	the commonly used chemical drug	1787:1817	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	1	7	theme	Ce-doped	249:256	arg1	Ce-ZnO/CH					275:283	Ce-ZnO/CH	275:283	Ce-ZnO/CH	275:283	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	1	7	theme	Ce-doped	249:256	arg1	nanoflowers					262:272	Ce-doped ZnO nanoflowers	249:272	Ce-doped ZnO nanoflowers (Ce-ZnO/CH)	249:284	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	0	8	theme	chitosan	100:107	arg1	matrix					109:114	ZnO nanoflowers impeded chitosan matrix	76:114	ZnO nanoflowers impeded chitosan matrix	76:114	Biological characterization of microwave based synthesized ZnO and Ce doped ZnO nanoflowers impeded chitosan matrix with enhanced antioxidant and anti-diabetic properties.					
37148946	3	9	theme	ZnO	585:587	arg1	particles					601:609	ZnO flower-like particles	585:609	ZnO flower-like particles	585:609	The integration of chitosan and cerium induced significantly the biological activity of ZnO flower-like particles.					
37148946	4	10	theme	strong	723:728	arg1	effect					730:735	the strong effect	719:735	the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate	719:867	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37148946	1	11	theme	ZnO	258:260	arg1	Ce-ZnO/CH					275:283	Ce-ZnO/CH	275:283	Ce-ZnO/CH	275:283	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	1	11	theme	ZnO	258:260	arg1	nanoflowers					262:272	Ce-doped ZnO nanoflowers	249:272	Ce-doped ZnO nanoflowers (Ce-ZnO/CH)	249:284	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	0	12	theme	impeded	92:98	arg1	matrix					109:114	ZnO nanoflowers impeded chitosan matrix	76:114	ZnO nanoflowers impeded chitosan matrix	76:114	Biological characterization of microwave based synthesized ZnO and Ce doped ZnO nanoflowers impeded chitosan matrix with enhanced antioxidant and anti-diabetic properties.					
37148946	3	13	theme	flower-like	589:599	arg1	particles					601:609	ZnO flower-like particles	585:609	ZnO flower-like particles	585:609	The integration of chitosan and cerium induced significantly the biological activity of ZnO flower-like particles.					
37148946	4	14	theme	higher	643:648	arg1	activities					650:659	higher activities	643:659	higher activities	643:659	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37148946	5	15	theme	Ascorbic	1127:1134	arg1	acid					1136:1139	Ascorbic acid	1127:1139	Ascorbic acid	1127:1139	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	16	theme	ABTS	1022:1025	arg1	radicals					1075:1082	nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals	992:1082	nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles	992:1195	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	16	theme	ABTS	1022:1025	arg1	DPPH					970:973	DPPH	970:973	DPPH (92.4 ± 1.33 %)	970:989	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	16	theme	ABTS	1022:1025	arg1	values					1115:1120	significantly higher values	1094:1120	significantly higher values than Ascorbic acid	1094:1139	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	8	17	theme	high	1744:1747	arg1	cost					1749:1752	the high cost	1740:1752	the high cost	1740:1752	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	2	18	theme	antidiabetic	414:425	arg1	structures					361:370	The obtained hybrid structures	341:370	The obtained hybrid structures	341:370	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	2	18	theme	antidiabetic	414:425	arg1	agents					427:432	antidiabetic agents	414:432	antidiabetic agents	414:432	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	7	19	theme	miglitol	1587:1594	arg1	drug					1596:1599	miglitol drug	1587:1599	miglitol drug	1587:1599	The recognized inhibition percentages are notably higher than the determined percentages using miglitol drug and slightly higher than acarbose.					
37148946	7	20	theme	determined	1558:1567	arg1	percentages					1569:1579	the determined percentages	1554:1579	the determined percentages using miglitol drug	1554:1599	The recognized inhibition percentages are notably higher than the determined percentages using miglitol drug and slightly higher than acarbose.					
37148946	4	21	theme	interactive	821:831	arg1	interface					833:841	the high interactive interface	812:841	the high interactive interface of the chitosan substrate	812:867	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37148946	5	22	theme	remarkable	931:940	arg1	antioxidant					876:886	an antioxidant	873:886	an antioxidant	873:886	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	22	theme	remarkable	931:940	arg1	efficiencies					953:964	remarkable scavenging efficiencies	931:964	remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles	931:1195	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	2	23	theme	different	475:483	arg1	components					485:494	the different components	471:494	the different components	471:494	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	4	24	theme	Ce-doped	612:619	arg1	nano-flowers					625:636	Ce-doped ZnO nano-flowers	612:636	Ce-doped ZnO nano-flowers	612:636	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37148946	5	25	theme	oxide	999:1003	arg1	radicals					1075:1082	nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals	992:1082	nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles	992:1195	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	25	theme	oxide	999:1003	arg1	DPPH					970:973	DPPH	970:973	DPPH (92.4 ± 1.33 %)	970:989	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	25	theme	oxide	999:1003	arg1	values					1115:1120	significantly higher values	1094:1120	significantly higher values than Ascorbic acid	1094:1139	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	26	theme	scavenging	942:951	arg1	antioxidant					876:886	an antioxidant	873:886	an antioxidant	873:886	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	26	theme	scavenging	942:951	arg1	efficiencies					953:964	remarkable scavenging efficiencies	931:964	remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles	931:1195	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	0	27	theme	Biological	0:9	arg1	characterization					11:26	Biological characterization	0:26	Biological characterization of microwave based	0:45	Biological characterization of microwave based synthesized ZnO and Ce doped ZnO nanoflowers impeded chitosan matrix with enhanced antioxidant and anti-diabetic properties.					
37148946	6	28	theme	porcine	1288:1294	arg1	α-amylase					1296:1304	porcine α-amylase	1288:1304	porcine α-amylase (93.6 ± 1.66 %)	1288:1320	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	28	theme	porcine	1288:1294	arg1	%					1319:1319	93.6 ± 1.66 %	1307:1319	93.6 ± 1.66 %	1307:1319	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	7	29	theme	inhibition	1507:1516	arg1	percentages					1518:1528	The recognized inhibition percentages	1492:1528	The recognized inhibition percentages	1492:1528	The recognized inhibition percentages are notably higher than the determined percentages using miglitol drug and slightly higher than acarbose.					
37148946	7	29	theme	inhibition	1507:1516	arg1	higher					1542:1547	higher	1542:1547	higher	1542:1547	The recognized inhibition percentages are notably higher than the determined percentages using miglitol drug and slightly higher than acarbose.					
37148946	4	30	theme	high	816:819	arg1	interface					833:841	the high interactive interface	812:841	the high interactive interface of the chitosan substrate	812:867	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37148946	2	31	theme	enhanced	389:396	arg1	antioxidant					398:408	enhanced antioxidant	389:408	enhanced antioxidant	389:408	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	2	31	theme	enhanced	389:396	arg1	structures					361:370	The obtained hybrid structures	341:370	The obtained hybrid structures	341:370	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	0	32	theme	microwave	31:39	arg1	based					41:45	microwave based	31:45	microwave based	31:45	Biological characterization of microwave based synthesized ZnO and Ce doped ZnO nanoflowers impeded chitosan matrix with enhanced antioxidant and anti-diabetic properties.					
37148946	0	33	theme	antioxidant	130:140	arg1	properties					160:169	enhanced antioxidant and anti-diabetic properties	121:169	enhanced antioxidant and anti-diabetic properties	121:169	Biological characterization of microwave based synthesized ZnO and Ce doped ZnO nanoflowers impeded chitosan matrix with enhanced antioxidant and anti-diabetic properties.					
37148946	5	34	theme	nitric	992:997	arg1	oxide					999:1003	nitric oxide	992:1003	nitric oxide (95.2 ± 1.81 %)	992:1019	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	34	theme	nitric	992:997	arg1	%					1018:1018	95.2 ± 1.81 %	1006:1018	95.2 ± 1.81 %	1006:1018	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	3	35	theme	particles	601:609	arg1	activity					573:580	the biological activity	558:580	the biological activity of ZnO flower-like particles	558:609	The integration of chitosan and cerium induced significantly the biological activity of ZnO flower-like particles.					
37148946	8	36	theme	reported	1762:1769	arg1	effects					1776:1782	the reported side effects	1758:1782	the reported side effects of the commonly used chemical drug	1758:1817	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	2	37	theme	synergetic	450:459	arg1	effect					461:466	the synergetic effect	446:466	the synergetic effect of the different components	446:494	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	8	38	theme	drug	1814:1817	arg1	effects					1776:1782	the reported side effects	1758:1782	the reported side effects of the commonly used chemical drug	1758:1817	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	8	38	theme	drug	1814:1817	arg1	cost					1749:1752	the high cost	1740:1752	the high cost	1740:1752	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	4	39	theme	ZnO	621:623	arg1	nano-flowers					625:636	Ce-doped ZnO nano-flowers	612:636	Ce-doped ZnO nano-flowers	612:636	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37148946	0	40	theme	enhanced	121:128	arg1	properties					160:169	enhanced antioxidant and anti-diabetic properties	121:169	enhanced antioxidant and anti-diabetic properties	121:169	Biological characterization of microwave based synthesized ZnO and Ce doped ZnO nanoflowers impeded chitosan matrix with enhanced antioxidant and anti-diabetic properties.					
37148946	6	41	theme	antidiabetic	1208:1219	arg1	efficiency					1221:1230	its antidiabetic efficiency	1204:1230	its antidiabetic efficiency	1204:1230	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	5	42	theme	synthetic	892:900	arg1	composite					912:920	the synthetic Ce-ZnO/CH composite	888:920	the synthetic Ce-ZnO/CH composite	888:920	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	6	43	theme	pancreatic	1356:1365	arg1	%					1394:1394	98.7 ± 1.26 %	1382:1394	98.7 ± 1.26 %	1382:1394	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	43	theme	pancreatic	1356:1365	arg1	α-glucosidase					1367:1379	pancreatic α-glucosidase	1356:1379	pancreatic α-glucosidase (98.7 ± 1.26 %)	1356:1395	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	3	44	theme	cerium	529:534	arg1	integration					501:511	The integration	497:511	The integration of chitosan and cerium	497:534	The integration of chitosan and cerium induced significantly the biological activity of ZnO flower-like particles.					
37148946	0	45	theme	anti-diabetic	146:158	arg1	properties					160:169	enhanced antioxidant and anti-diabetic properties	121:169	enhanced antioxidant and anti-diabetic properties	121:169	Biological characterization of microwave based synthesized ZnO and Ce doped ZnO nanoflowers impeded chitosan matrix with enhanced antioxidant and anti-diabetic properties.					
37148946	5	46	theme	Ce-ZnO/CH	902:910	arg1	composite					912:920	the synthetic Ce-ZnO/CH composite	888:920	the synthetic Ce-ZnO/CH composite	888:920	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	0	47	theme	based	41:45	arg1	characterization					11:26	Biological characterization	0:26	Biological characterization of microwave based	0:45	Biological characterization of microwave based synthesized ZnO and Ce doped ZnO nanoflowers impeded chitosan matrix with enhanced antioxidant and anti-diabetic properties.					
37148946	1	48	theme	microwave-induced	289:305	arg1	processes					330:338	microwave-induced hydrothermal synthesis processes	289:338	microwave-induced hydrothermal synthesis processes	289:338	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	8	49	theme	Ce-ZnO/CH	1656:1664	arg1	composite					1666:1674	the Ce-ZnO/CH composite	1652:1674	the Ce-ZnO/CH composite	1652:1674	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	8	49	theme	Ce-ZnO/CH	1656:1664	arg1	agent					1720:1724	a potential antidiabetic and antioxidant agent	1679:1724	a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug	1679:1817	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	5	50	theme	higher	1108:1113	arg1	radicals					1075:1082	nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals	992:1082	nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles	992:1195	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	50	theme	higher	1108:1113	arg1	values					1115:1120	significantly higher values	1094:1120	significantly higher values than Ascorbic acid	1094:1139	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	51	theme	superoxide	1048:1057	arg1	radicals					1075:1082	nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals	992:1082	nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles	992:1195	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	51	theme	superoxide	1048:1057	arg1	DPPH					970:973	DPPH	970:973	DPPH (92.4 ± 1.33 %)	970:989	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	5	51	theme	superoxide	1048:1057	arg1	values					1115:1120	significantly higher values	1094:1120	significantly higher values than Ascorbic acid	1094:1139	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	1	52	theme	ZnO	220:222	arg1	ZnO/CH					237:242	ZnO/CH	237:242	ZnO/CH	237:242	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	1	52	theme	ZnO	220:222	arg1	nanoflowers					224:234	ZnO nanoflowers	220:234	ZnO nanoflowers (ZnO/CH)	220:243	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	6	53	dep	enhanced	1232:1239	arg1	achieving					1249:1257	achieving	1249:1257	enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes	1232:1489	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	1	54	theme	hydrothermal	307:318	arg1	processes					330:338	microwave-induced hydrothermal synthesis processes	289:338	microwave-induced hydrothermal synthesis processes	289:338	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	6	55	theme	inhibition	1266:1275	arg1	effects					1277:1283	strong inhibition effects	1259:1283	strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes	1259:1489	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	2	56	theme	hybrid	354:359	arg1	agents					427:432	antidiabetic agents	414:432	antidiabetic agents	414:432	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	2	56	theme	hybrid	354:359	arg1	antioxidant					398:408	enhanced antioxidant	389:408	enhanced antioxidant	389:408	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	2	56	theme	hybrid	354:359	arg1	structures					361:370	The obtained hybrid structures	341:370	The obtained hybrid structures	341:370	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	2	57	theme	components	485:494	arg1	effect					461:466	the synergetic effect	446:466	the synergetic effect of the different components	446:494	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	1	58	theme	synthesis	320:328	arg1	processes					330:338	microwave-induced hydrothermal synthesis processes	289:338	microwave-induced hydrothermal synthesis processes	289:338	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	7	59	theme	recognized	1496:1505	arg1	percentages					1518:1528	The recognized inhibition percentages	1492:1528	The recognized inhibition percentages	1492:1528	The recognized inhibition percentages are notably higher than the determined percentages using miglitol drug and slightly higher than acarbose.					
37148946	7	59	theme	recognized	1496:1505	arg1	higher					1542:1547	higher	1542:1547	higher	1542:1547	The recognized inhibition percentages are notably higher than the determined percentages using miglitol drug and slightly higher than acarbose.					
37148946	6	60	theme	strong	1259:1264	arg1	effects					1277:1283	strong inhibition effects	1259:1283	strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes	1259:1489	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	5	61	theme	used	1174:1177	arg1	nanoparticles					1183:1195	the commercially used ZnO nanoparticles	1157:1195	the commercially used ZnO nanoparticles	1157:1195	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	2	62	theme	obtained	345:352	arg1	agents					427:432	antidiabetic agents	414:432	antidiabetic agents	414:432	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	2	62	theme	obtained	345:352	arg1	antioxidant					398:408	enhanced antioxidant	389:408	enhanced antioxidant	389:408	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	2	62	theme	obtained	345:352	arg1	structures					361:370	The obtained hybrid structures	341:370	The obtained hybrid structures	341:370	The obtained hybrid structures were assessed as enhanced antioxidant and antidiabetic agents considering the synergetic effect of the different components.					
37148946	4	63	theme	surface	740:746	arg1	electrons					748:756	surface electrons	740:756	surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate	740:867	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37148946	4	64	theme	doping	782:787	arg1	process					789:795	the doping process	778:795	the doping process	778:795	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37148946	8	65	theme	potential	1681:1689	arg1	composite					1666:1674	the Ce-ZnO/CH composite	1652:1674	the Ce-ZnO/CH composite	1652:1674	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	8	65	theme	potential	1681:1689	arg1	agent					1720:1724	a potential antidiabetic and antioxidant agent	1679:1724	a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug	1679:1817	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	8	66	theme	used	1800:1803	arg1	drug					1814:1817	the commonly used chemical drug	1787:1817	the commonly used chemical drug	1787:1817	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	1	67	used	used	196:199	arg2	matrix					185:190	The chitosan matrix	172:190	The chitosan matrix	172:190	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	1	67	used	used	196:199	arg2	substrate					206:214	a substrate	204:214	a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH)	204:284	The chitosan matrix was used as a substrate for ZnO nanoflowers (ZnO/CH) and Ce-doped ZnO nanoflowers (Ce-ZnO/CH) by microwave-induced hydrothermal synthesis processes.					
37148946	3	68	theme	chitosan	516:523	arg1	integration					501:511	The integration	497:511	The integration of chitosan and cerium	497:534	The integration of chitosan and cerium induced significantly the biological activity of ZnO flower-like particles.					
37148946	8	69	theme	antioxidant	1708:1718	arg1	composite					1666:1674	the Ce-ZnO/CH composite	1652:1674	the Ce-ZnO/CH composite	1652:1674	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	8	69	theme	antioxidant	1708:1718	arg1	agent					1720:1724	a potential antidiabetic and antioxidant agent	1679:1724	a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug	1679:1817	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	6	70	dep	α-amylase	1296:1304	arg1	enzymes					1483:1489	enzymes	1483:1489	enzymes	1483:1489	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	8	71	theme	side	1771:1774	arg1	effects					1776:1782	the reported side effects	1758:1782	the reported side effects of the commonly used chemical drug	1758:1817	This recommends the Ce-ZnO/CH composite as a potential antidiabetic and antioxidant agent compared with the high cost and the reported side effects of the commonly used chemical drug.					
37148946	4	72	theme	substrate	859:867	arg1	interface					833:841	the high interactive interface	812:841	the high interactive interface of the chitosan substrate	812:867	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37148946	4	73	theme	ZnO/CH	691:696	arg1	composite					698:706	ZnO/CH composite	691:706	ZnO/CH composite	691:706	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37148946	4	74	theme	electrons	748:756	arg1	effect					730:735	the strong effect	719:735	the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate	719:867	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37148946	6	75	from	effects	1277:1283	arg1	%					1442:1442	96.8 ± 1.16 %	1430:1442	96.8 ± 1.16 %	1430:1442	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	75	from	effects	1277:1283	arg1	α-amylase					1296:1304	porcine α-amylase	1288:1304	porcine α-amylase (93.6 ± 1.66 %)	1288:1320	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	75	from	effects	1277:1283	arg1	α-glucosidase					1367:1379	pancreatic α-glucosidase	1356:1379	pancreatic α-glucosidase (98.7 ± 1.26 %)	1356:1395	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	75	from	effects	1277:1283	arg1	α-amylase					1329:1337	crude α-amylase	1323:1337	crude α-amylase (88.7 ± 1.82 %)	1323:1353	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	75	from	effects	1277:1283	arg1	α-glucosidase					1415:1427	crude intestinal α-glucosidase	1398:1427	crude intestinal α-glucosidase (96.8 ± 1.16 %)	1398:1443	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	75	from	effects	1277:1283	arg1	%					1352:1352	88.7 ± 1.82 %	1340:1352	88.7 ± 1.82 %	1340:1352	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	75	from	effects	1277:1283	arg1	amyloglucosidase					1450:1465	amyloglucosidase	1450:1465	amyloglucosidase (97.2 ± 1.72 %)	1450:1481	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	75	from	effects	1277:1283	arg1	%					1319:1319	93.6 ± 1.66 %	1307:1319	93.6 ± 1.66 %	1307:1319	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	75	from	effects	1277:1283	arg1	%					1394:1394	98.7 ± 1.26 %	1382:1394	98.7 ± 1.26 %	1382:1394	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	75	from	effects	1277:1283	arg1	%					1480:1480	97.2 ± 1.72 %	1468:1480	97.2 ± 1.72 %	1468:1480	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	76	theme	intestinal	1404:1413	arg1	%					1442:1442	96.8 ± 1.16 %	1430:1442	96.8 ± 1.16 %	1430:1442	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	6	76	theme	intestinal	1404:1413	arg1	α-glucosidase					1415:1427	crude intestinal α-glucosidase	1398:1427	crude intestinal α-glucosidase (96.8 ± 1.16 %)	1398:1443	Also, its antidiabetic efficiency enhanced greatly achieving strong inhibition effects on porcine α-amylase (93.6 ± 1.66 %), crude α-amylase (88.7 ± 1.82 %), pancreatic α-glucosidase (98.7 ± 1.26 %), crude intestinal α-glucosidase (96.8 ± 1.16 %), and amyloglucosidase (97.2 ± 1.72 %) enzymes.					
37148946	5	77	theme	ZnO	1179:1181	arg1	nanoparticles					1183:1195	the commercially used ZnO nanoparticles	1157:1195	the commercially used ZnO nanoparticles	1157:1195	As an antioxidant the synthetic Ce-ZnO/CH composite achieved remarkable scavenging efficiencies for DPPH (92.4 ± 1.33 %), nitric oxide (95.2 ± 1.81 %), ABTS (90.4 ± 1.64 %), and superoxide (52.8 ± 1.22 %) radicals which are significantly higher values than Ascorbic acid as standard and the commercially used ZnO nanoparticles.					
37148946	4	78	theme	chitosan	850:857	arg1	substrate					859:867	the chitosan substrate	846:867	the chitosan substrate	846:867	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37148946	3	79	theme	biological	562:571	arg1	activity					573:580	the biological activity	558:580	the biological activity of ZnO flower-like particles	558:609	The integration of chitosan and cerium induced significantly the biological activity of ZnO flower-like particles.					
37148946	4	80	theme	ZnO	671:673	arg1	nanoflowers					675:685	ZnO nanoflowers	671:685	ZnO nanoflowers	671:685	Ce-doped ZnO nano-flowers show higher activities than both ZnO nanoflowers and ZnO/CH composite reflecting the strong effect of surface electrons that were formed by the doping process as compared to the high interactive interface of the chitosan substrate.					
37321738	7	0	theme	loaded	1263:1268	arg1	films					1283:1287	Co-BIT loaded CA composite films	1256:1287	Co-BIT loaded CA composite films	1256:1287	These findings suggest that Co-BIT loaded CA composite films have great potential for use as smart active packaging.					
37321738	7	1	contain	have	1289:1292	arg2	potential					1300:1308	great potential	1294:1308	great potential for use	1294:1316	These findings suggest that Co-BIT loaded CA composite films have great potential for use as smart active packaging.					
37321738	7	1	contain	have	1289:1292	arg1	films					1283:1287	Co-BIT loaded CA composite films	1256:1287	Co-BIT loaded CA composite films	1256:1287	These findings suggest that Co-BIT loaded CA composite films have great potential for use as smart active packaging.					
37321738	4	2	theme	mechanical	724:733	arg1	strength					735:742	mechanical strength	724:742	mechanical strength (from 24.12 to 39.76 MPa)	724:768	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	1	3	theme	packaging	170:178	arg1	materials					180:188	smart packaging materials	164:188	smart packaging materials capable of effectively monitoring the food freshness	164:241	Nowadays, there is an increasing demand for smart packaging materials capable of effectively monitoring the food freshness.					
37321738	0	4	theme	packaging	97:105	arg1	application					107:117	smart packaging application	91:117	smart packaging application	91:117	Ammonia-sensitive cellulose acetate-based films incorporated with Co-BIT microcrystals for smart packaging application.					
37321738	1	5	dep	Nowadays	120:127	arg1	there					130:134	there	130:134	there	130:134	Nowadays, there is an increasing demand for smart packaging materials capable of effectively monitoring the food freshness.					
37321738	3	6	theme	functional	528:537	arg1	structure					503:511	structure	503:511	structure	503:511	The influences of Co-BIT loading upon structure, physical, and functional properties of the CA films were then thoroughly explored.					
37321738	3	6	theme	functional	528:537	arg1	properties					539:548	physical, and functional properties	514:548	physical, and functional properties of the CA films	514:564	The influences of Co-BIT loading upon structure, physical, and functional properties of the CA films were then thoroughly explored.					
37321738	3	7	theme	CA	557:558	arg1	films					560:564	the CA films	553:564	the CA films	553:564	The influences of Co-BIT loading upon structure, physical, and functional properties of the CA films were then thoroughly explored.					
37321738	7	8	theme	Co-BIT	1256:1261	arg1	films					1283:1287	Co-BIT loaded CA composite films	1256:1287	Co-BIT loaded CA composite films	1256:1287	These findings suggest that Co-BIT loaded CA composite films have great potential for use as smart active packaging.					
37321738	4	9	theme	water	771:775	arg1	barrier					777:783	water barrier	771:783	water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa)	771:826	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	7	10	theme	great	1294:1298	arg1	potential					1300:1308	great potential	1294:1308	great potential for use	1294:1316	These findings suggest that Co-BIT loaded CA composite films have great potential for use as smart active packaging.					
37321738	3	11	theme	films	560:564	arg1	structure					503:511	structure	503:511	structure	503:511	The influences of Co-BIT loading upon structure, physical, and functional properties of the CA films were then thoroughly explored.					
37321738	3	11	theme	films	560:564	arg1	properties					539:548	physical, and functional properties	514:548	physical, and functional properties of the CA films	514:564	The influences of Co-BIT loading upon structure, physical, and functional properties of the CA films were then thoroughly explored.					
37321738	7	12	theme	smart	1321:1325	arg1	packaging					1334:1342	smart active packaging	1321:1342	smart active packaging	1321:1342	These findings suggest that Co-BIT loaded CA composite films have great potential for use as smart active packaging.					
37321738	3	13	theme	loading	490:496	arg1	influences					469:478	The influences	465:478	The influences of Co-BIT loading upon structure, physical, and functional properties of the CA films	465:564	The influences of Co-BIT loading upon structure, physical, and functional properties of the CA films were then thoroughly explored.					
37321738	1	14	theme	capable	190:196	arg1	materials					180:188	smart packaging materials	164:188	smart packaging materials capable of effectively monitoring the food freshness	164:241	Nowadays, there is an increasing demand for smart packaging materials capable of effectively monitoring the food freshness.					
37321738	2	15	theme	Co-BIT	277:282	arg1	microcrystals					285:297	new Co-based MOF (Co-BIT) microcrystals	259:297	new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function	259:349	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37321738	4	16	dep	2.73 × 10-6 g/m·h·Pa	806:825	arg1	9.32 × 10-6					791:801	9.32 × 10-6	791:801	9.32 × 10-6	791:801	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	0	17	theme	cellulose	18:26	arg1	films					42:46	Ammonia-sensitive cellulose acetate-based films	0:46	Ammonia-sensitive cellulose acetate-based films	0:46	Ammonia-sensitive cellulose acetate-based films incorporated with Co-BIT microcrystals for smart packaging application.					
37321738	7	18	theme	CA	1270:1271	arg1	films					1283:1287	Co-BIT loaded CA composite films	1256:1287	Co-BIT loaded CA composite films	1256:1287	These findings suggest that Co-BIT loaded CA composite films have great potential for use as smart active packaging.					
37321738	4	19	theme	film	880:883	arg1	barrier					777:783	water barrier	771:783	water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa)	771:826	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	4	19	theme	film	880:883	arg1	light					844:848	ultraviolet light	832:848	ultraviolet light	832:848	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	4	19	theme	film	880:883	arg1	strength					735:742	mechanical strength	724:742	mechanical strength (from 24.12 to 39.76 MPa)	724:768	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	7	20	theme	composite	1273:1281	arg1	films					1283:1287	Co-BIT loaded CA composite films	1256:1287	Co-BIT loaded CA composite films	1256:1287	These findings suggest that Co-BIT loaded CA composite films have great potential for use as smart active packaging.					
37321738	6	21	theme	discernible	1201:1211	arg1	changes					1219:1225	discernible color changes	1201:1225	discernible color changes	1201:1225	Finally, the CA/Co-BIT films were successfully applied for indicating the spoilage of shrimp through discernible color changes.					
37321738	2	22	theme	CA	410:411	arg1	matrix					414:419	cellulose acetate (CA) matrix	391:419	cellulose acetate (CA) matrix to create smart active packaging materials	391:462	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37321738	0	23	theme	Ammonia-sensitive	0:16	arg1	films					42:46	Ammonia-sensitive cellulose acetate-based films	0:46	Ammonia-sensitive cellulose acetate-based films	0:46	Ammonia-sensitive cellulose acetate-based films incorporated with Co-BIT microcrystals for smart packaging application.					
37321738	4	24	theme	CA	877:878	arg1	film					880:883	CA film	877:883	CA film	877:883	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	2	25	theme	antibacterial	328:340	arg1	function					342:349	antibacterial function	328:349	antibacterial function	328:349	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37321738	2	26	dep	matrix	414:419	arg1	create					424:429	create	424:429	to create smart active packaging materials	421:462	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37321738	6	27	theme	shrimp	1186:1191	arg1	spoilage					1174:1181	the spoilage	1170:1181	the spoilage of shrimp	1170:1191	Finally, the CA/Co-BIT films were successfully applied for indicating the spoilage of shrimp through discernible color changes.					
37321738	5	28	theme	ammonia-sensitivity	1043:1061	arg1	function					1063:1070	favorable ammonia-sensitivity function	1033:1070	favorable ammonia-sensitivity function as well as color stability	1033:1097	Additionally, the created CA/Co-BIT films displayed striking antibacterial efficacy (>95.0 % for both Escherichia coli and Staphylococcus aureus), favorable ammonia-sensitivity function as well as color stability.					
37321738	2	29	theme	active	437:442	arg1	materials					454:462	smart active packaging materials	431:462	smart active packaging materials	431:462	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37321738	2	30	theme	packaging	444:452	arg1	materials					454:462	smart active packaging materials	431:462	smart active packaging materials	431:462	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37321738	4	31	dep	strength	735:742	arg1	performances					861:872	protection performances	850:872	protection performances	850:872	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	1	32	theme	food	228:231	arg1	freshness					233:241	the food freshness	224:241	the food freshness	224:241	Nowadays, there is an increasing demand for smart packaging materials capable of effectively monitoring the food freshness.					
37321738	5	33	theme	antibacterial	947:959	arg1	efficacy					961:968	striking antibacterial efficacy	938:968	striking antibacterial efficacy	938:968	Additionally, the created CA/Co-BIT films displayed striking antibacterial efficacy (>95.0 % for both Escherichia coli and Staphylococcus aureus), favorable ammonia-sensitivity function as well as color stability.					
37321738	4	34	theme	ultraviolet	832:842	arg1	light					844:848	ultraviolet light	832:848	ultraviolet light	832:848	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	4	35	theme	CA	674:675	arg1	matrix					677:682	CA matrix	674:682	CA matrix	674:682	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	0	36	theme	acetate-based	28:40	arg1	films					42:46	Ammonia-sensitive cellulose acetate-based films	0:46	Ammonia-sensitive cellulose acetate-based films	0:46	Ammonia-sensitive cellulose acetate-based films incorporated with Co-BIT microcrystals for smart packaging application.					
37321738	6	37	theme	CA/Co-BIT	1113:1121	arg1	films					1123:1127	the CA/Co-BIT films	1109:1127	the CA/Co-BIT films	1109:1127	Finally, the CA/Co-BIT films were successfully applied for indicating the spoilage of shrimp through discernible color changes.					
37321738	4	38	from	2.73 × 10-6 g/m·h·Pa	806:825	arg1	barrier					777:783	water barrier	771:783	water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa)	771:826	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	4	38	from	2.73 × 10-6 g/m·h·Pa	806:825	arg1	light					844:848	ultraviolet light	832:848	ultraviolet light	832:848	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	4	38	from	2.73 × 10-6 g/m·h·Pa	806:825	arg1	strength					735:742	mechanical strength	724:742	mechanical strength (from 24.12 to 39.76 MPa)	724:768	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	5	39	theme	created	904:910	arg1	films					922:926	the created CA/Co-BIT films	900:926	the created CA/Co-BIT films	900:926	Additionally, the created CA/Co-BIT films displayed striking antibacterial efficacy (>95.0 % for both Escherichia coli and Staphylococcus aureus), favorable ammonia-sensitivity function as well as color stability.					
37321738	4	40	theme	protection	850:859	arg1	performances					861:872	protection performances	850:872	protection performances	850:872	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	5	41	dep	displayed	928:936	arg1	%					977:977	>95.0 %	971:977	>95.0 % for both Escherichia coli and Staphylococcus aureus	971:1029	Additionally, the created CA/Co-BIT films displayed striking antibacterial efficacy (>95.0 % for both Escherichia coli and Staphylococcus aureus), favorable ammonia-sensitivity function as well as color stability.					
37321738	5	42	theme	striking	938:945	arg1	efficacy					961:968	striking antibacterial efficacy	938:968	striking antibacterial efficacy	938:968	Additionally, the created CA/Co-BIT films displayed striking antibacterial efficacy (>95.0 % for both Escherichia coli and Staphylococcus aureus), favorable ammonia-sensitivity function as well as color stability.					
37321738	0	43	theme	Co-BIT	66:71	arg1	microcrystals					73:85	Co-BIT microcrystals	66:85	Co-BIT microcrystals for smart packaging application	66:117	Ammonia-sensitive cellulose acetate-based films incorporated with Co-BIT microcrystals for smart packaging application.					
37321738	3	44	theme	physical	514:521	arg1	structure					503:511	structure	503:511	structure	503:511	The influences of Co-BIT loading upon structure, physical, and functional properties of the CA films were then thoroughly explored.					
37321738	3	44	theme	physical	514:521	arg1	properties					539:548	physical, and functional properties	514:548	physical, and functional properties of the CA films	514:564	The influences of Co-BIT loading upon structure, physical, and functional properties of the CA films were then thoroughly explored.					
37321738	5	45	theme	favorable	1033:1041	arg1	function					1063:1070	favorable ammonia-sensitivity function	1033:1070	favorable ammonia-sensitivity function as well as color stability	1033:1097	Additionally, the created CA/Co-BIT films displayed striking antibacterial efficacy (>95.0 % for both Escherichia coli and Staphylococcus aureus), favorable ammonia-sensitivity function as well as color stability.					
37321738	5	46	theme	color	1083:1087	arg1	stability					1089:1097	color stability	1083:1097	favorable ammonia-sensitivity function as well as color stability	1033:1097	Additionally, the created CA/Co-BIT films displayed striking antibacterial efficacy (>95.0 % for both Escherichia coli and Staphylococcus aureus), favorable ammonia-sensitivity function as well as color stability.					
37321738	4	47	theme	significant	698:708	arg1	promotions					710:719	significant promotions	698:719	significant promotions	698:719	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	1	48	theme	increasing	142:151	arg1	demand					153:158	an increasing demand	139:158	an increasing demand for smart packaging materials capable of effectively monitoring the food freshness	139:241	Nowadays, there is an increasing demand for smart packaging materials capable of effectively monitoring the food freshness.					
37321738	4	49	theme	microcrystalline	618:633	arg1	Co-BIT					635:640	microcrystalline Co-BIT	618:640	microcrystalline Co-BIT	618:640	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	6	50	theme	color	1213:1217	arg1	changes					1219:1225	discernible color changes	1201:1225	discernible color changes	1201:1225	Finally, the CA/Co-BIT films were successfully applied for indicating the spoilage of shrimp through discernible color changes.					
37321738	7	51	theme	active	1327:1332	arg1	packaging					1334:1342	smart active packaging	1321:1342	smart active packaging	1321:1342	These findings suggest that Co-BIT loaded CA composite films have great potential for use as smart active packaging.					
37321738	2	52	theme	MOF	272:274	arg1	microcrystals					285:297	new Co-based MOF (Co-BIT) microcrystals	259:297	new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function	259:349	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37321738	1	53	theme	smart	164:168	arg1	materials					180:188	smart packaging materials	164:188	smart packaging materials capable of effectively monitoring the food freshness	164:241	Nowadays, there is an increasing demand for smart packaging materials capable of effectively monitoring the food freshness.					
37321738	4	54	dep	39.76 MPa	759:767	arg1	24.12					750:754	24.12	750:754	24.12	750:754	It was observed that microcrystalline Co-BIT was uniformly integrated inside CA matrix, which caused significant promotions in mechanical strength (from 24.12 to 39.76 MPa), water barrier (from 9.32 × 10-6 to 2.73 × 10-6 g/m·h·Pa) and ultraviolet light protection performances of CA film.					
37321738	0	55	theme	smart	91:95	arg1	application					107:117	smart packaging application	91:117	smart packaging application	91:117	Ammonia-sensitive cellulose acetate-based films incorporated with Co-BIT microcrystals for smart packaging application.					
37321738	5	56	theme	CA/Co-BIT	912:920	arg1	films					922:926	the created CA/Co-BIT films	900:926	the created CA/Co-BIT films	900:926	Additionally, the created CA/Co-BIT films displayed striking antibacterial efficacy (>95.0 % for both Escherichia coli and Staphylococcus aureus), favorable ammonia-sensitivity function as well as color stability.					
37321738	2	57	theme	Co-based	263:270	arg1	microcrystals					285:297	new Co-based MOF (Co-BIT) microcrystals	259:297	new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function	259:349	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37321738	3	58	theme	Co-BIT	483:488	arg1	loading					490:496	Co-BIT loading	483:496	Co-BIT loading	483:496	The influences of Co-BIT loading upon structure, physical, and functional properties of the CA films were then thoroughly explored.					
37321738	2	59	theme	acetate	401:407	arg1	matrix					414:419	cellulose acetate (CA) matrix	391:419	cellulose acetate (CA) matrix to create smart active packaging materials	391:462	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37321738	2	60	theme	new	259:261	arg1	microcrystals					285:297	new Co-based MOF (Co-BIT) microcrystals	259:297	new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function	259:349	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37321738	2	61	theme	cellulose	391:399	arg1	matrix					414:419	cellulose acetate (CA) matrix	391:419	cellulose acetate (CA) matrix to create smart active packaging materials	391:462	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37321738	2	62	with	microcrystals	285:297	arg1	ammonia-sensitivity					304:322	ammonia-sensitivity	304:322	ammonia-sensitivity	304:322	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37321738	2	62	with	microcrystals	285:297	arg1	function					342:349	antibacterial function	328:349	antibacterial function	328:349	In this study, new Co-based MOF (Co-BIT) microcrystals with ammonia-sensitivity and antibacterial function were constructed and then loaded within cellulose acetate (CA) matrix to create smart active packaging materials.					
37043370	2	0	theme	critical-size	392:404	arg1	defects					411:417	critical-size bone defects	392:417	critical-size bone defects	392:417	Implantation of grafts is often required to promote the regeneration of critical-size bone defects.					
37043370	9	1	theme	endothelial	1454:1464	arg1	cells					1466:1470	endothelial cells	1454:1470	endothelial cells	1454:1470	The slow release of strontium ions can effectively promote mesenchymal stem cells osteogenic differentiation and the vascularization of endothelial cells.					
37043370	0	2	theme	Bone	123:126	arg1	Regeneration					128:139	Bone Regeneration	123:139	Bone Regeneration	123:139	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres Promote Osteogenesis and Angiogenesis for Bone Regeneration.					
37043370	4	3	theme	Strontium	533:541	arg1	Sr2+					549:552	Sr2+	549:552	Sr2+	549:552	Strontium ions (Sr2+) can promote osteogenesis and angiogenesis.					
37043370	4	3	theme	Strontium	533:541	arg1	ions					543:546	Strontium ions	533:546	Strontium ions (Sr2+)	533:553	Strontium ions (Sr2+) can promote osteogenesis and angiogenesis.					
37043370	7	4	theme	ions	1099:1102	arg1	release					1078:1084	the release	1074:1084	the release of strontium ions	1074:1102	The coordination reaction of PDA can effectively control the release of strontium ions and avoid the negative effects caused by the high strontium concentration.					
37043370	9	5	theme	cells	1466:1470	arg1	vascularization					1435:1449	the vascularization	1431:1449	the vascularization of endothelial cells	1431:1470	The slow release of strontium ions can effectively promote mesenchymal stem cells osteogenic differentiation and the vascularization of endothelial cells.					
37043370	9	5	theme	cells	1466:1470	arg1	differentiation					1411:1425	mesenchymal stem cells osteogenic differentiation	1377:1425	mesenchymal stem cells osteogenic differentiation	1377:1425	The slow release of strontium ions can effectively promote mesenchymal stem cells osteogenic differentiation and the vascularization of endothelial cells.					
37043370	5	6	theme	extracellular	730:742	arg1	structure					766:774	an extracellular matrix-like nanofiber structure	727:774	an extracellular matrix-like nanofiber structure	727:774	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	6	7	theme	metal	936:940	arg1	ions					942:945	metal ions	936:945	metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity	936:1014	Strontium ions were stably added into the microspheres by using polydopamine (PDA) to chelate metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity.					
37043370	5	8	theme	composite	658:666	arg1	CS					654:655	CS	654:655	CS	654:655	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	5	8	theme	composite	658:666	arg1	microspheres					668:679	nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres	618:679	nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres	618:679	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	6	9	theme	good	999:1002	arg1	bioactivity					1004:1014	good bioactivity	999:1014	good bioactivity	999:1014	Strontium ions were stably added into the microspheres by using polydopamine (PDA) to chelate metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity.					
37043370	5	10	theme	matrix-like	744:754	arg1	structure					766:774	an extracellular matrix-like nanofiber structure	727:774	an extracellular matrix-like nanofiber structure	727:774	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	11	11	theme	bone	1652:1655	arg1	regeneration					1657:1668	bone regeneration	1652:1668	bone regeneration	1652:1668	The results showed that nHA/CS@PDA-Sr could effectively promote bone regeneration in the defect area.					
37043370	3	12	theme	tissue	516:521	arg1	function					523:530	the tissue function	512:530	the tissue function	512:530	Metal ions play an important role in human health, as they affect the body's metabolism and the tissue function.					
37043370	5	13	theme	nanofiber	756:764	arg1	structure					766:774	an extracellular matrix-like nanofiber structure	727:774	an extracellular matrix-like nanofiber structure	727:774	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	10	14	theme	cranial	1526:1532	arg1	defects					1534:1540	cranial defects	1526:1540	cranial defects of rats	1526:1548	In addition, we injected composite microspheres into cranial defects of rats to evaluate osseointegration in vivo.					
37043370	6	15	theme	Strontium	842:850	arg1	ions					852:855	Strontium ions	842:855	Strontium ions	842:855	Strontium ions were stably added into the microspheres by using polydopamine (PDA) to chelate metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity.					
37043370	7	16	theme	high	1149:1152	arg1	concentration					1164:1176	the high strontium concentration	1145:1176	the high strontium concentration	1145:1176	The coordination reaction of PDA can effectively control the release of strontium ions and avoid the negative effects caused by the high strontium concentration.					
37043370	9	17	theme	strontium	1338:1346	arg1	ions					1348:1351	strontium ions	1338:1351	strontium ions	1338:1351	The slow release of strontium ions can effectively promote mesenchymal stem cells osteogenic differentiation and the vascularization of endothelial cells.					
37043370	7	18	theme	strontium	1154:1162	arg1	concentration					1164:1176	the high strontium concentration	1145:1176	the high strontium concentration	1145:1176	The coordination reaction of PDA can effectively control the release of strontium ions and avoid the negative effects caused by the high strontium concentration.					
37043370	11	19	theme	@	1618:1618	arg1	PDA-Sr					1619:1624	nHA/CS@PDA-Sr	1612:1624	nHA/CS@PDA-Sr	1612:1624	The results showed that nHA/CS@PDA-Sr could effectively promote bone regeneration in the defect area.					
37043370	9	20	theme	osteogenic	1400:1409	arg1	differentiation					1411:1425	mesenchymal stem cells osteogenic differentiation	1377:1425	mesenchymal stem cells osteogenic differentiation	1377:1425	The slow release of strontium ions can effectively promote mesenchymal stem cells osteogenic differentiation and the vascularization of endothelial cells.					
37043370	0	21	theme	Ion-Functionalized	10:27	arg1	Microspheres					68:79	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres	0:79	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres	0:79	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres Promote Osteogenesis and Angiogenesis for Bone Regeneration.					
37043370	9	22	theme	ions	1348:1351	arg1	release					1327:1333	The slow release	1318:1333	The slow release of strontium ions	1318:1351	The slow release of strontium ions can effectively promote mesenchymal stem cells osteogenic differentiation and the vascularization of endothelial cells.					
37043370	5	23	theme	/chitosan	643:651	arg1	CS					654:655	CS	654:655	CS	654:655	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	5	23	theme	/chitosan	643:651	arg1	microspheres					668:679	nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres	618:679	nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres	618:679	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	1	24	from	problem	186:192	arg1	practice					206:213	clinical practice	197:213	clinical practice	197:213	Critical-size bone defects are an important problem in clinical practice, which usually occurs in severe trauma, or tumor resection, and cannot heal completely and autonomously.					
37043370	12	25	theme	composite	1719:1727	arg1	microspheres					1729:1740	composite microspheres	1719:1740	composite microspheres	1719:1740	This study demonstrates that composite microspheres stimulate bone repair providing a promising way for bone-defect regeneration.					
37043370	3	26	theme	human	457:461	arg1	health					463:468	human health	457:468	human health	457:468	Metal ions play an important role in human health, as they affect the body's metabolism and the tissue function.					
37043370	0	27	theme	Strontium	0:8	arg1	Microspheres					68:79	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres	0:79	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres	0:79	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres Promote Osteogenesis and Angiogenesis for Bone Regeneration.					
37043370	10	28	theme	composite	1498:1506	arg1	microspheres					1508:1519	composite microspheres	1498:1519	composite microspheres	1498:1519	In addition, we injected composite microspheres into cranial defects of rats to evaluate osseointegration in vivo.					
37043370	1	29	theme	Critical-size	142:154	arg1	problem					186:192	an important problem	173:192	an important problem	173:192	Critical-size bone defects are an important problem in clinical practice, which usually occurs in severe trauma, or tumor resection, and cannot heal completely and autonomously.					
37043370	1	29	theme	Critical-size	142:154	arg1	defects					161:167	Critical-size bone defects	142:167	Critical-size bone defects	142:167	Critical-size bone defects are an important problem in clinical practice, which usually occurs in severe trauma, or tumor resection, and cannot heal completely and autonomously.					
37043370	8	30	dep	in	1183:1184	arg1	vitro					1186:1190	vitro	1186:1190	vitro	1186:1190	Our in vitro experiments showed that the composite microspheres had good biocompatibility and that the PDA coating promotes cell adhesion.					
37043370	0	31	theme	Composite	58:66	arg1	Microspheres					68:79	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres	0:79	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres	0:79	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres Promote Osteogenesis and Angiogenesis for Bone Regeneration.					
37043370	5	32	theme	size	705:708	arg1	distribution					710:721	a uniform particle size distribution	686:721	a uniform particle size distribution	686:721	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	11	33	theme	nHA/CS	1612:1617	arg1	PDA-Sr					1619:1624	nHA/CS@PDA-Sr	1612:1624	nHA/CS@PDA-Sr	1612:1624	The results showed that nHA/CS@PDA-Sr could effectively promote bone regeneration in the defect area.					
37043370	1	34	theme	bone	156:159	arg1	problem					186:192	an important problem	173:192	an important problem	173:192	Critical-size bone defects are an important problem in clinical practice, which usually occurs in severe trauma, or tumor resection, and cannot heal completely and autonomously.					
37043370	1	34	theme	bone	156:159	arg1	defects					161:167	Critical-size bone defects	142:167	Critical-size bone defects	142:167	Critical-size bone defects are an important problem in clinical practice, which usually occurs in severe trauma, or tumor resection, and cannot heal completely and autonomously.					
37043370	1	35	theme	severe	240:245	arg1	trauma					247:252	severe trauma	240:252	severe trauma	240:252	Critical-size bone defects are an important problem in clinical practice, which usually occurs in severe trauma, or tumor resection, and cannot heal completely and autonomously.					
37043370	3	36	theme	Metal	420:424	arg1	ions					426:429	Metal ions	420:429	Metal ions	420:429	Metal ions play an important role in human health, as they affect the body's metabolism and the tissue function.					
37043370	6	37	theme	@	985:985	arg1	material					969:976	a bone repair material	955:976	a bone repair material (nHA/CS@PDA-Sr)	955:992	Strontium ions were stably added into the microspheres by using polydopamine (PDA) to chelate metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity.					
37043370	6	37	theme	@	985:985	arg1	PDA-Sr					986:991	nHA/CS@PDA-Sr	979:991	nHA/CS@PDA-Sr	979:991	Strontium ions were stably added into the microspheres by using polydopamine (PDA) to chelate metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity.					
37043370	7	38	theme	strontium	1089:1097	arg1	ions					1099:1102	strontium ions	1089:1102	strontium ions	1089:1102	The coordination reaction of PDA can effectively control the release of strontium ions and avoid the negative effects caused by the high strontium concentration.					
37043370	2	39	theme	grafts	336:341	arg1	Implantation					320:331	Implantation	320:331	Implantation of grafts	320:341	Implantation of grafts is often required to promote the regeneration of critical-size bone defects.					
37043370	8	40	theme	PDA	1282:1284	arg1	coating					1286:1292	the PDA coating	1278:1292	the PDA coating	1278:1292	Our in vitro experiments showed that the composite microspheres had good biocompatibility and that the PDA coating promotes cell adhesion.					
37043370	5	41	theme	direct	810:815	arg1	gelation					832:839	direct alkali-induced gelation	810:839	direct alkali-induced gelation	810:839	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	6	42	theme	nHA/CS	979:984	arg1	material					969:976	a bone repair material	955:976	a bone repair material (nHA/CS@PDA-Sr)	955:992	Strontium ions were stably added into the microspheres by using polydopamine (PDA) to chelate metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity.					
37043370	6	42	theme	nHA/CS	979:984	arg1	PDA-Sr					986:991	nHA/CS@PDA-Sr	979:991	nHA/CS@PDA-Sr	979:991	Strontium ions were stably added into the microspheres by using polydopamine (PDA) to chelate metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity.					
37043370	8	43	contain	had	1243:1245	arg1	microspheres					1230:1241	the composite microspheres	1216:1241	the composite microspheres	1216:1241	Our in vitro experiments showed that the composite microspheres had good biocompatibility and that the PDA coating promotes cell adhesion.					
37043370	8	43	contain	had	1243:1245	arg2	biocompatibility					1252:1267	good biocompatibility	1247:1267	good biocompatibility	1247:1267	Our in vitro experiments showed that the composite microspheres had good biocompatibility and that the PDA coating promotes cell adhesion.					
37043370	8	44	theme	cell	1303:1306	arg1	adhesion					1308:1315	cell adhesion	1303:1315	cell adhesion	1303:1315	Our in vitro experiments showed that the composite microspheres had good biocompatibility and that the PDA coating promotes cell adhesion.					
37043370	5	45	theme	alkali-induced	817:830	arg1	gelation					832:839	direct alkali-induced gelation	810:839	direct alkali-induced gelation	810:839	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	9	46	theme	stem	1389:1392	arg1	cells					1394:1398	mesenchymal stem cells	1377:1398	mesenchymal stem cells osteogenic differentiation	1377:1425	The slow release of strontium ions can effectively promote mesenchymal stem cells osteogenic differentiation and the vascularization of endothelial cells.					
37043370	8	47	theme	composite	1220:1228	arg1	microspheres					1230:1241	the composite microspheres	1216:1241	the composite microspheres	1216:1241	Our in vitro experiments showed that the composite microspheres had good biocompatibility and that the PDA coating promotes cell adhesion.					
37043370	9	48	theme	slow	1322:1325	arg1	release					1327:1333	The slow release	1318:1333	The slow release of strontium ions	1318:1351	The slow release of strontium ions can effectively promote mesenchymal stem cells osteogenic differentiation and the vascularization of endothelial cells.					
37043370	8	49	theme	in	1183:1184	arg1	experiments					1192:1202	Our in vitro experiments	1179:1202	Our in vitro experiments	1179:1202	Our in vitro experiments showed that the composite microspheres had good biocompatibility and that the PDA coating promotes cell adhesion.					
37043370	8	50	theme	good	1247:1250	arg1	biocompatibility					1252:1267	good biocompatibility	1247:1267	good biocompatibility	1247:1267	Our in vitro experiments showed that the composite microspheres had good biocompatibility and that the PDA coating promotes cell adhesion.					
37043370	9	51	theme	cells	1394:1398	arg1	differentiation					1411:1425	mesenchymal stem cells osteogenic differentiation	1377:1425	mesenchymal stem cells osteogenic differentiation	1377:1425	The slow release of strontium ions can effectively promote mesenchymal stem cells osteogenic differentiation and the vascularization of endothelial cells.					
37043370	7	52	theme	PDA	1046:1048	arg1	reaction					1034:1041	The coordination reaction	1017:1041	The coordination reaction of PDA	1017:1048	The coordination reaction of PDA can effectively control the release of strontium ions and avoid the negative effects caused by the high strontium concentration.					
37043370	3	53	theme	important	439:447	arg1	role					449:452	an important role	436:452	an important role	436:452	Metal ions play an important role in human health, as they affect the body's metabolism and the tissue function.					
37043370	6	54	theme	repair	962:967	arg1	material					969:976	a bone repair material	955:976	a bone repair material (nHA/CS@PDA-Sr)	955:992	Strontium ions were stably added into the microspheres by using polydopamine (PDA) to chelate metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity.					
37043370	6	54	theme	repair	962:967	arg1	PDA-Sr					986:991	nHA/CS@PDA-Sr	979:991	nHA/CS@PDA-Sr	979:991	Strontium ions were stably added into the microspheres by using polydopamine (PDA) to chelate metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity.					
37043370	12	55	theme	bone-defect	1794:1804	arg1	regeneration					1806:1817	bone-defect regeneration	1794:1817	bone-defect regeneration	1794:1817	This study demonstrates that composite microspheres stimulate bone repair providing a promising way for bone-defect regeneration.					
37043370	7	56	theme	negative	1118:1125	arg1	effects					1127:1133	the negative effects	1114:1133	the negative effects caused by the high strontium concentration	1114:1176	The coordination reaction of PDA can effectively control the release of strontium ions and avoid the negative effects caused by the high strontium concentration.					
37043370	12	57	theme	promising	1776:1784	arg1	way					1786:1788	a promising way	1774:1788	a promising way for bone-defect regeneration	1774:1817	This study demonstrates that composite microspheres stimulate bone repair providing a promising way for bone-defect regeneration.					
37043370	6	58	theme	bone	957:960	arg1	material					969:976	a bone repair material	955:976	a bone repair material (nHA/CS@PDA-Sr)	955:992	Strontium ions were stably added into the microspheres by using polydopamine (PDA) to chelate metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity.					
37043370	6	58	theme	bone	957:960	arg1	PDA-Sr					986:991	nHA/CS@PDA-Sr	979:991	nHA/CS@PDA-Sr	979:991	Strontium ions were stably added into the microspheres by using polydopamine (PDA) to chelate metal ions forming a bone repair material (nHA/CS@PDA-Sr) with good bioactivity.					
37043370	5	59	theme	uniform	688:694	arg1	distribution					710:721	a uniform particle size distribution	686:721	a uniform particle size distribution	686:721	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	9	60	theme	mesenchymal	1377:1387	arg1	cells					1394:1398	mesenchymal stem cells	1377:1398	mesenchymal stem cells osteogenic differentiation	1377:1425	The slow release of strontium ions can effectively promote mesenchymal stem cells osteogenic differentiation and the vascularization of endothelial cells.					
37043370	7	61	theme	coordination	1021:1032	arg1	reaction					1034:1041	The coordination reaction	1017:1041	The coordination reaction of PDA	1017:1048	The coordination reaction of PDA can effectively control the release of strontium ions and avoid the negative effects caused by the high strontium concentration.					
37043370	1	62	theme	important	176:184	arg1	problem					186:192	an important problem	173:192	an important problem	173:192	Critical-size bone defects are an important problem in clinical practice, which usually occurs in severe trauma, or tumor resection, and cannot heal completely and autonomously.					
37043370	1	62	theme	important	176:184	arg1	defects					161:167	Critical-size bone defects	142:167	Critical-size bone defects	142:167	Critical-size bone defects are an important problem in clinical practice, which usually occurs in severe trauma, or tumor resection, and cannot heal completely and autonomously.					
37043370	1	63	theme	tumor	258:262	arg1	resection					264:272	tumor resection	258:272	tumor resection	258:272	Critical-size bone defects are an important problem in clinical practice, which usually occurs in severe trauma, or tumor resection, and cannot heal completely and autonomously.					
37043370	5	64	theme	particle	696:703	arg1	distribution					710:721	a uniform particle size distribution	686:721	a uniform particle size distribution	686:721	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	2	65	theme	bone	406:409	arg1	defects					411:417	critical-size bone defects	392:417	critical-size bone defects	392:417	Implantation of grafts is often required to promote the regeneration of critical-size bone defects.					
37043370	10	66	theme	rats	1545:1548	arg1	defects					1534:1540	cranial defects	1526:1540	cranial defects of rats	1526:1548	In addition, we injected composite microspheres into cranial defects of rats to evaluate osseointegration in vivo.					
37043370	11	67	theme	defect	1677:1682	arg1	area					1684:1687	the defect area	1673:1687	the defect area	1673:1687	The results showed that nHA/CS@PDA-Sr could effectively promote bone regeneration in the defect area.					
37043370	2	68	theme	defects	411:417	arg1	regeneration					376:387	the regeneration	372:387	the regeneration of critical-size bone defects	372:417	Implantation of grafts is often required to promote the regeneration of critical-size bone defects.					
37043370	5	69	theme	microfluidic	782:793	arg1	technology					795:804	microfluidic technology	782:804	microfluidic technology	782:804	Herein, we prepared nano-hydroxyapatite (nHA)/chitosan (CS) composite microspheres with a uniform particle size distribution and an extracellular matrix-like nanofiber structure using microfluidic technology and direct alkali-induced gelation.					
37043370	0	70	theme	Nano-Hydroxyapatite/Chitosan	29:56	arg1	Microspheres					68:79	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres	0:79	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres	0:79	Strontium Ion-Functionalized Nano-Hydroxyapatite/Chitosan Composite Microspheres Promote Osteogenesis and Angiogenesis for Bone Regeneration.					
37043370	1	71	theme	clinical	197:204	arg1	practice					206:213	clinical practice	197:213	clinical practice	197:213	Critical-size bone defects are an important problem in clinical practice, which usually occurs in severe trauma, or tumor resection, and cannot heal completely and autonomously.					
37043370	12	72	theme	bone	1752:1755	arg1	repair					1757:1762	bone repair	1752:1762	bone repair	1752:1762	This study demonstrates that composite microspheres stimulate bone repair providing a promising way for bone-defect regeneration.					
37049481	2	0	theme	colonic	549:555	arg1	incubations					557:567	short-term colonic incubations	538:567	short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO	538:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	1	1	with	patients	228:235	arg1	syndrome					258:265	irritable bowel syndrome	242:265	irritable bowel syndrome (IBS)	242:271	Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) cause intestinal discomfort in patients with irritable bowel syndrome (IBS).					
37049481	1	1	with	patients	228:235	arg1	IBS					268:270	IBS	268:270	IBS	268:270	Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) cause intestinal discomfort in patients with irritable bowel syndrome (IBS).					
37049481	4	2	with	patients	1007:1014	arg1	IBS-D					1021:1025	IBS-D	1021:1025	IBS-D	1021:1025	A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME®), using fecal samples from two patients with IBS-D, further evaluated these findings.					
37049481	8	3	theme	IBS	1502:1504	arg1	symptoms					1506:1513	FODMAP-related IBS symptoms	1487:1513	FODMAP-related IBS symptoms	1487:1513	When used in combination, the FODMAP enzyme mix and HMO may resolve FODMAP-related IBS symptoms while maintaining a healthy gut microbiome via prebiotic activity.					
37049481	2	4	dep	FODMAP	768:773	arg1	iv					751:752	iv	751:752	iv	751:752	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	5	theme	fecal	579:583	arg1	microbiota					585:594	the fecal microbiota	575:594	the fecal microbiota of four patients with IBS-D symptoms	575:631	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	6	dep	oligosaccharides	413:428	arg1	oligosaccharides					413:428	human milk oligosaccharides	402:428	human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT)	402:457	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	6	dep	oligosaccharides	413:428	arg1	DFL					444:446	DFL	444:446	DFL	444:446	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	6	dep	oligosaccharides	413:428	arg1	LNnT					453:456	LNnT	453:456	LNnT	453:456	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	6	dep	oligosaccharides	413:428	arg1	2'-FL					437:441	2'-FL	437:441	2'-FL	437:441	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	4	7	theme	microbial	948:956	arg1	SHIME®					969:974	SHIME®	969:974	SHIME®	969:974	A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME®), using fecal samples from two patients with IBS-D, further evaluated these findings.					
37049481	4	7	theme	microbial	948:956	arg1	ecosystem					958:966	human intestinal microbial ecosystem	931:966	the human intestinal microbial ecosystem (SHIME®)	927:975	A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME®), using fecal samples from two patients with IBS-D, further evaluated these findings.					
37049481	4	8	theme	10-day	887:892	arg1	experiment					894:903	A 10-day experiment	885:903	A 10-day experiment	885:903	A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME®), using fecal samples from two patients with IBS-D, further evaluated these findings.					
37049481	2	9	theme	test	653:656	arg1	HMO					777:779	HMO	777:779	HMO	777:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	9	theme	test	653:656	arg1	FODMAP					674:679	(i) FODMAP	670:679	(i) FODMAP	670:679	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	9	theme	test	653:656	arg1	conditions					658:667	the following test conditions	639:667	the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO	639:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	9	theme	test	653:656	arg1	FODMAP					768:773	(iv) pre-digested FODMAP	750:773	(iv) pre-digested FODMAP	750:773	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	1	10	theme	Fermentable	113:123	arg1	oligosaccharides					125:140	Fermentable oligosaccharides	113:140	Fermentable oligosaccharides	113:140	Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) cause intestinal discomfort in patients with irritable bowel syndrome (IBS).					
37049481	1	10	theme	Fermentable	113:123	arg1	FODMAPs					188:194	FODMAPs	188:194	FODMAPs	188:194	Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) cause intestinal discomfort in patients with irritable bowel syndrome (IBS).					
37049481	2	11	theme	community	500:508	arg1	activity					510:517	microbial community activity	490:517	microbial community activity	490:517	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	6	12	theme	enzyme	1148:1153	arg1	mix					1155:1157	the enzyme mix	1144:1157	the enzyme mix	1144:1157	Pre-digestion with the enzyme mix restored microbial diversity, improved FODMAP digestibility, and reduced gas pressure versus undigested FODMAP; however, SCFA production decreased.					
37049481	0	13	theme	Oligosaccharides	42:57	arg1	Effect					9:14	In Vitro Effect	0:14	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.	0:111	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.					
37049481	0	14	from	Effect	9:14	arg1	Digestion					69:77	FODMAP Digestion	62:77	FODMAP Digestion	62:77	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.					
37049481	0	14	from	Effect	9:14	arg1	Composition					100:110	Fecal Microbiota Composition	83:110	Fecal Microbiota Composition	83:110	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.					
37049481	0	15	theme	FODMAP	62:67	arg1	Digestion					69:77	FODMAP Digestion	62:77	FODMAP Digestion	62:77	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.					
37049481	2	16	from	activity	510:517	arg1	incubations					557:567	short-term colonic incubations	538:567	short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO	538:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	0	17	theme	In	0:1	arg1	Effect					9:14	In Vitro Effect	0:14	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.	0:111	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.					
37049481	4	18	with	experiment	894:903	arg1	simulator					914:922	the simulator	910:922	the simulator of the human intestinal microbial ecosystem (SHIME®)	910:975	A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME®), using fecal samples from two patients with IBS-D, further evaluated these findings.					
37049481	2	19	theme	milk	408:411	arg1	oligosaccharides					413:428	human milk oligosaccharides	402:428	human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT)	402:457	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	19	theme	milk	408:411	arg1	HMO					431:433	HMO	431:433	HMO	431:433	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	19	theme	milk	408:411	arg1	DFL					444:446	DFL	444:446	DFL	444:446	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	19	theme	milk	408:411	arg1	LNnT					453:456	LNnT	453:456	LNnT	453:456	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	19	theme	milk	408:411	arg1	2'-FL					437:441	2'-FL	437:441	2'-FL	437:441	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	20	from	composition	523:533	arg1	incubations					557:567	short-term colonic incubations	538:567	short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO	538:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	6	21	theme	SCFA	1280:1283	arg1	production					1285:1294	SCFA production	1280:1294	SCFA production	1280:1294	Pre-digestion with the enzyme mix restored microbial diversity, improved FODMAP digestibility, and reduced gas pressure versus undigested FODMAP; however, SCFA production decreased.					
37049481	2	22	dep	conditions	658:667	arg1	FODMAP					732:737	(iii) FODMAP	726:737	(iii) FODMAP	726:737	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	22	dep	conditions	658:667	arg1	FODMAP					718:723	(ii) pre-digested (with enzyme mix) FODMAP	682:723	(ii) pre-digested (with enzyme mix) FODMAP	682:723	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	22	dep	conditions	658:667	arg1	FODMAP					768:773	(iv) pre-digested FODMAP	750:773	(iv) pre-digested FODMAP	750:773	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	22	dep	conditions	658:667	arg1	FODMAP					674:679	(i) FODMAP	670:679	(i) FODMAP	670:679	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	22	dep	conditions	658:667	arg1	HMO					777:779	HMO	777:779	HMO	777:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	22	dep	conditions	658:667	arg1	conditions					658:667	the following test conditions	639:667	the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO	639:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	23	theme	pre-digested	755:766	arg1	conditions					658:667	the following test conditions	639:667	the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO	639:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	23	theme	pre-digested	755:766	arg1	FODMAP					768:773	(iv) pre-digested FODMAP	750:773	(iv) pre-digested FODMAP	750:773	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	0	24	theme	Enzymes	19:25	arg1	Effect					9:14	In Vitro Effect	0:14	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.	0:111	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.					
37049481	7	25	theme	increased	1379:1387	arg1	abundance					1389:1397	increased abundance	1379:1397	increased abundance of Lachnospiraceae	1379:1416	HMO restored SCFA production along with an increase in gas pressure and increased abundance of Lachnospiraceae.					
37049481	3	26	theme	acid	828:831	arg1	production					840:849	short-chain fatty acid (SCFA) production	810:849	short-chain fatty acid (SCFA) production	810:849	Pre-digested FODMAP reduced short-chain fatty acid (SCFA) production versus FODMAP; HMO restored this.					
37049481	2	27	theme	GalU	313:316	arg1	α-galactosidase					318:332	2400 GalU α-galactosidase	308:332	2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO	308:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	0	28	theme	Human	31:35	arg1	Oligosaccharides					42:57	Human Milk Oligosaccharides	31:57	Human Milk Oligosaccharides	31:57	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.					
37049481	3	29	theme	short-chain	810:820	arg1	acid					828:831	short-chain fatty acid	810:831	short-chain fatty acid (SCFA) production	810:849	Pre-digested FODMAP reduced short-chain fatty acid (SCFA) production versus FODMAP; HMO restored this.					
37049481	3	29	theme	short-chain	810:820	arg1	SCFA					834:837	SCFA	834:837	SCFA	834:837	Pre-digested FODMAP reduced short-chain fatty acid (SCFA) production versus FODMAP; HMO restored this.					
37049481	2	30	dep	FODMAP	732:737	arg1	iii					727:729	iii	727:729	iii	727:729	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	4	31	theme	intestinal	937:946	arg1	SHIME®					969:974	SHIME®	969:974	SHIME®	969:974	A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME®), using fecal samples from two patients with IBS-D, further evaluated these findings.					
37049481	4	31	theme	intestinal	937:946	arg1	ecosystem					958:966	human intestinal microbial ecosystem	931:966	the human intestinal microbial ecosystem (SHIME®)	927:975	A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME®), using fecal samples from two patients with IBS-D, further evaluated these findings.					
37049481	2	32	theme	FODMAP	377:382	arg1	digestion					384:392	FODMAP digestion	377:392	FODMAP digestion	377:392	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	33	theme	ALU	342:344	arg1	β-galactosidase					346:360	10,000 ALU β-galactosidase	335:360	10,000 ALU β-galactosidase	335:360	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	0	34	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.					
37049481	2	35	from	effects	479:485	arg1	composition					523:533	composition	523:533	composition	523:533	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	35	from	effects	479:485	arg1	activity					510:517	microbial community activity	490:517	microbial community activity	490:517	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	8	36	theme	healthy	1535:1541	arg1	microbiome					1547:1556	a healthy gut microbiome	1533:1556	a healthy gut microbiome	1533:1556	When used in combination, the FODMAP enzyme mix and HMO may resolve FODMAP-related IBS symptoms while maintaining a healthy gut microbiome via prebiotic activity.					
37049481	1	37	theme	bowel	252:256	arg1	syndrome					258:265	irritable bowel syndrome	242:265	irritable bowel syndrome (IBS)	242:271	Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) cause intestinal discomfort in patients with irritable bowel syndrome (IBS).					
37049481	1	37	theme	bowel	252:256	arg1	IBS					268:270	IBS	268:270	IBS	268:270	Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) cause intestinal discomfort in patients with irritable bowel syndrome (IBS).					
37049481	1	38	theme	irritable	242:250	arg1	syndrome					258:265	irritable bowel syndrome	242:265	irritable bowel syndrome (IBS)	242:271	Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) cause intestinal discomfort in patients with irritable bowel syndrome (IBS).					
37049481	1	38	theme	irritable	242:250	arg1	IBS					268:270	IBS	268:270	IBS	268:270	Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) cause intestinal discomfort in patients with irritable bowel syndrome (IBS).					
37049481	2	39	theme	short-term	538:547	arg1	incubations					557:567	short-term colonic incubations	538:567	short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO	538:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	40	theme	enzyme	277:282	arg1	mix					284:286	An enzyme mix	274:286	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.	274:780	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	7	41	from	abundance	1389:1397	arg1	pressure					1366:1373	gas pressure	1362:1373	gas pressure	1362:1373	HMO restored SCFA production along with an increase in gas pressure and increased abundance of Lachnospiraceae.					
37049481	7	42	theme	SCFA	1320:1323	arg1	production					1325:1334	SCFA production	1320:1334	SCFA production	1320:1334	HMO restored SCFA production along with an increase in gas pressure and increased abundance of Lachnospiraceae.					
37049481	8	43	theme	FODMAP-related	1487:1500	arg1	symptoms					1506:1513	FODMAP-related IBS symptoms	1487:1513	FODMAP-related IBS symptoms	1487:1513	When used in combination, the FODMAP enzyme mix and HMO may resolve FODMAP-related IBS symptoms while maintaining a healthy gut microbiome via prebiotic activity.					
37049481	8	44	theme	prebiotic	1562:1570	arg1	activity					1572:1579	prebiotic activity	1562:1579	prebiotic activity	1562:1579	When used in combination, the FODMAP enzyme mix and HMO may resolve FODMAP-related IBS symptoms while maintaining a healthy gut microbiome via prebiotic activity.					
37049481	4	45	theme	fecal	984:988	arg1	samples					990:996	fecal samples	984:996	fecal samples from two patients with IBS-D	984:1025	A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME®), using fecal samples from two patients with IBS-D, further evaluated these findings.					
37049481	6	46	theme	gas	1232:1234	arg1	pressure					1236:1243	gas pressure	1232:1243	gas pressure	1232:1243	Pre-digestion with the enzyme mix restored microbial diversity, improved FODMAP digestibility, and reduced gas pressure versus undigested FODMAP; however, SCFA production decreased.					
37049481	2	47	theme	SU	294:295	arg1	invertase					297:305	2500 SU invertase	289:305	2500 SU invertase	289:305	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	48	dep	FODMAP	718:723	arg1	ii					683:684	ii	683:684	ii	683:684	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	4	49	theme	ecosystem	958:966	arg1	simulator					914:922	the simulator	910:922	the simulator of the human intestinal microbial ecosystem (SHIME®)	910:975	A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME®), using fecal samples from two patients with IBS-D, further evaluated these findings.					
37049481	6	50	theme	FODMAP	1198:1203	arg1	digestibility					1205:1217	FODMAP digestibility	1198:1217	FODMAP digestibility	1198:1217	Pre-digestion with the enzyme mix restored microbial diversity, improved FODMAP digestibility, and reduced gas pressure versus undigested FODMAP; however, SCFA production decreased.					
37049481	6	51	dep	restored	1159:1166	arg1	decreased					1296:1304	decreased	1296:1304	decreased	1296:1304	Pre-digestion with the enzyme mix restored microbial diversity, improved FODMAP digestibility, and reduced gas pressure versus undigested FODMAP; however, SCFA production decreased.					
37049481	2	52	theme	pre-digested	687:698	arg1	FODMAP					718:723	(ii) pre-digested (with enzyme mix) FODMAP	682:723	(ii) pre-digested (with enzyme mix) FODMAP	682:723	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	53	theme	enzyme	706:711	arg1	mix					713:715	enzyme mix	706:715	enzyme mix	706:715	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	54	with	FODMAP	718:723	arg1	mix					713:715	enzyme mix	706:715	enzyme mix	706:715	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	1	55	theme	intestinal	203:212	arg1	discomfort					214:223	intestinal discomfort	203:223	intestinal discomfort in patients with irritable bowel syndrome (IBS)	203:271	Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) cause intestinal discomfort in patients with irritable bowel syndrome (IBS).					
37049481	6	56	theme	microbial	1168:1176	arg1	diversity					1178:1186	microbial diversity	1168:1186	microbial diversity	1168:1186	Pre-digestion with the enzyme mix restored microbial diversity, improved FODMAP digestibility, and reduced gas pressure versus undigested FODMAP; however, SCFA production decreased.					
37049481	1	57	from	discomfort	214:223	arg1	patients					228:235	patients	228:235	patients with irritable bowel syndrome (IBS)	228:271	Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) cause intestinal discomfort in patients with irritable bowel syndrome (IBS).					
37049481	7	58	from	increase	1350:1357	arg1	pressure					1366:1373	gas pressure	1362:1373	gas pressure	1362:1373	HMO restored SCFA production along with an increase in gas pressure and increased abundance of Lachnospiraceae.					
37049481	2	59	theme	following	643:651	arg1	HMO					777:779	HMO	777:779	HMO	777:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	59	theme	following	643:651	arg1	FODMAP					674:679	(i) FODMAP	670:679	(i) FODMAP	670:679	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	59	theme	following	643:651	arg1	conditions					658:667	the following test conditions	639:667	the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO	639:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	59	theme	following	643:651	arg1	FODMAP					768:773	(iv) pre-digested FODMAP	750:773	(iv) pre-digested FODMAP	750:773	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	60	dep	mix	284:286	arg1	invertase					297:305	2500 SU invertase	289:305	2500 SU invertase	289:305	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	61	theme	microbial	490:498	arg1	activity					510:517	microbial community activity	490:517	microbial community activity	490:517	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	6	62	theme	undigested	1252:1261	arg1	FODMAP					1263:1268	undigested FODMAP	1252:1268	undigested FODMAP	1252:1268	Pre-digestion with the enzyme mix restored microbial diversity, improved FODMAP digestibility, and reduced gas pressure versus undigested FODMAP; however, SCFA production decreased.					
37049481	5	63	theme	microbial	1091:1099	arg1	diversity					1101:1109	decreased microbial diversity	1081:1109	decreased microbial diversity	1081:1109	FODMAP resulted in decreased microbial diversity versus blank.					
37049481	2	64	with	patients	604:611	arg1	symptoms					624:631	IBS-D symptoms	618:631	IBS-D symptoms	618:631	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	65	theme	patients	604:611	arg1	microbiota					585:594	the fecal microbiota	575:594	the fecal microbiota of four patients with IBS-D symptoms	575:631	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	0	66	theme	Fecal	83:87	arg1	Composition					100:110	Fecal Microbiota Composition	83:110	Fecal Microbiota Composition	83:110	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.					
37049481	2	67	dep	α-galactosidase	318:332	arg1	optimized					363:371	optimized	363:371	optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT)	363:457	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	67	dep	α-galactosidase	318:332	arg1	β-galactosidase					346:360	10,000 ALU β-galactosidase	335:360	10,000 ALU β-galactosidase	335:360	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	8	68	dep	enzyme	1456:1461	arg1	HMO					1471:1473	HMO	1471:1473	HMO	1471:1473	When used in combination, the FODMAP enzyme mix and HMO may resolve FODMAP-related IBS symptoms while maintaining a healthy gut microbiome via prebiotic activity.					
37049481	8	68	dep	enzyme	1456:1461	arg1	mix					1463:1465	mix	1463:1465	mix	1463:1465	When used in combination, the FODMAP enzyme mix and HMO may resolve FODMAP-related IBS symptoms while maintaining a healthy gut microbiome via prebiotic activity.					
37049481	2	69	theme	IBS-D	618:622	arg1	symptoms					624:631	IBS-D symptoms	618:631	IBS-D symptoms	618:631	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	70	dep	invertase	297:305	arg1	α-galactosidase					318:332	2400 GalU α-galactosidase	308:332	2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO	308:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	71	theme	human	402:406	arg1	oligosaccharides					413:428	human milk oligosaccharides	402:428	human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT)	402:457	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	71	theme	human	402:406	arg1	HMO					431:433	HMO	431:433	HMO	431:433	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	71	theme	human	402:406	arg1	DFL					444:446	DFL	444:446	DFL	444:446	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	71	theme	human	402:406	arg1	LNnT					453:456	LNnT	453:456	LNnT	453:456	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	2	71	theme	human	402:406	arg1	2'-FL					437:441	2'-FL	437:441	2'-FL	437:441	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	7	72	theme	Lachnospiraceae	1402:1416	arg1	abundance					1389:1397	increased abundance	1379:1397	increased abundance of Lachnospiraceae	1379:1416	HMO restored SCFA production along with an increase in gas pressure and increased abundance of Lachnospiraceae.					
37049481	7	72	theme	Lachnospiraceae	1402:1416	arg1	increase					1350:1357	an increase	1347:1357	an increase in gas pressure	1347:1373	HMO restored SCFA production along with an increase in gas pressure and increased abundance of Lachnospiraceae.					
37049481	3	73	theme	Pre-digested	782:793	arg1	FODMAP					795:800	Pre-digested FODMAP	782:800	Pre-digested FODMAP	782:800	Pre-digested FODMAP reduced short-chain fatty acid (SCFA) production versus FODMAP; HMO restored this.					
37049481	0	74	theme	Microbiota	89:98	arg1	Composition					100:110	Fecal Microbiota Composition	83:110	Fecal Microbiota Composition	83:110	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.					
37049481	0	75	theme	Milk	37:40	arg1	Oligosaccharides					42:57	Human Milk Oligosaccharides	31:57	Human Milk Oligosaccharides	31:57	In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.					
37049481	8	76	theme	FODMAP	1449:1454	arg1	enzyme					1456:1461	the FODMAP enzyme mix and HMO	1445:1473	the FODMAP enzyme mix and HMO	1445:1473	When used in combination, the FODMAP enzyme mix and HMO may resolve FODMAP-related IBS symptoms while maintaining a healthy gut microbiome via prebiotic activity.					
37049481	6	77	with	Pre-digestion	1125:1137	arg1	mix					1155:1157	the enzyme mix	1144:1157	the enzyme mix	1144:1157	Pre-digestion with the enzyme mix restored microbial diversity, improved FODMAP digestibility, and reduced gas pressure versus undigested FODMAP; however, SCFA production decreased.					
37049481	4	78	from	patients	1007:1014	arg1	samples					990:996	fecal samples	984:996	fecal samples from two patients with IBS-D	984:1025	A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME®), using fecal samples from two patients with IBS-D, further evaluated these findings.					
37049481	3	79	theme	fatty	822:826	arg1	acid					828:831	short-chain fatty acid	810:831	short-chain fatty acid (SCFA) production	810:849	Pre-digested FODMAP reduced short-chain fatty acid (SCFA) production versus FODMAP; HMO restored this.					
37049481	3	79	theme	fatty	822:826	arg1	SCFA					834:837	SCFA	834:837	SCFA	834:837	Pre-digested FODMAP reduced short-chain fatty acid (SCFA) production versus FODMAP; HMO restored this.					
37049481	7	80	theme	gas	1362:1364	arg1	pressure					1366:1373	gas pressure	1362:1373	gas pressure	1362:1373	HMO restored SCFA production along with an increase in gas pressure and increased abundance of Lachnospiraceae.					
37049481	2	81	dep	FODMAP	674:679	arg1	i					671:671	i	671:671	i	671:671	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	4	82	theme	human	931:935	arg1	SHIME®					969:974	SHIME®	969:974	SHIME®	969:974	A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME®), using fecal samples from two patients with IBS-D, further evaluated these findings.					
37049481	4	82	theme	human	931:935	arg1	ecosystem					958:966	human intestinal microbial ecosystem	931:966	the human intestinal microbial ecosystem (SHIME®)	927:975	A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME®), using fecal samples from two patients with IBS-D, further evaluated these findings.					
37049481	2	83	dep	optimized	363:371	arg1	evaluated					465:473	evaluated	465:473	were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO	460:779	An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO.					
37049481	8	84	theme	gut	1543:1545	arg1	microbiome					1547:1556	a healthy gut microbiome	1533:1556	a healthy gut microbiome	1533:1556	When used in combination, the FODMAP enzyme mix and HMO may resolve FODMAP-related IBS symptoms while maintaining a healthy gut microbiome via prebiotic activity.					
37049481	5	85	theme	decreased	1081:1089	arg1	diversity					1101:1109	decreased microbial diversity	1081:1109	decreased microbial diversity	1081:1109	FODMAP resulted in decreased microbial diversity versus blank.					
35839908	10	0	used	used	1406:1409	arg2	genomes					1393:1399	six potential cellulolytic genomes	1366:1399	six potential cellulolytic genomes	1366:1399	Further, twenty-two metagenome-assembled genomes (MAGs) were assembled from metagenomes and six potential cellulolytic genomes were used to reconstruct the cellulose-degrading process, revealing the potential metabolic cooperation related to cellulose degradation.					
35839908	5	1	theme	starch	683:688	arg1	contents					662:669	contents	662:669	contents of protein, starch, pectin, lignin, and cellulose	662:719	The results showed that contents of protein, starch, pectin, lignin, and cellulose varied in fermented leaves from different growing sites.					
35839908	10	2	theme	cellulose-degrading	1430:1448	arg1	process					1450:1456	the cellulose-degrading process	1426:1456	the cellulose-degrading process	1426:1456	Further, twenty-two metagenome-assembled genomes (MAGs) were assembled from metagenomes and six potential cellulolytic genomes were used to reconstruct the cellulose-degrading process, revealing the potential metabolic cooperation related to cellulose degradation.					
35839908	1	3	theme	benign	164:169	arg1	part					192:195	an environmentally benign and energy efficient part	145:195	an environmentally benign and energy efficient part in product processing	145:217	Microbial degradation of organic compounds is an environmentally benign and energy efficient part in product processing.					
35839908	1	3	theme	benign	164:169	arg1	degradation					109:119	Microbial degradation	99:119	Microbial degradation of organic compounds	99:140	Microbial degradation of organic compounds is an environmentally benign and energy efficient part in product processing.					
35839908	3	4	theme	natural	346:352	arg1	communities					366:376	natural degradation communities	346:376	natural degradation communities	346:376	However, microbes and enzymes discovered from natural degradation communities were still limited by cultural methods.					
35839908	8	5	theme	Pantoea	1129:1135	arg1	ananatis					1137:1144	Pantoea ananatis	1129:1144	Pantoea ananatis	1129:1144	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
35839908	8	6	theme	protein	1038:1044	arg1	degradations					1022:1033	degradations	1022:1033	degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp	1022:1199	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
35839908	6	7	theme	communities	900:910	arg1	composition					844:854	taxonomic composition	834:854	taxonomic composition	834:854	The different compound contents were closely related to taxonomic composition and functional profiles of foliar microbial communities.					
35839908	6	7	theme	communities	900:910	arg1	profiles					871:878	functional profiles	860:878	functional profiles	860:878	The different compound contents were closely related to taxonomic composition and functional profiles of foliar microbial communities.					
35839908	4	8	theme	fermented	612:620	arg1	tobacco					622:628	fermented tobacco leaves	612:635	fermented tobacco leaves	612:635	In this study, we used a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in compound degradation and explore the potential synergy among community members in fermented tobacco leaves.					
35839908	7	9	with	correlations	954:965	arg1	cellulose					993:1001	cellulose	993:1001	cellulose	993:1001	Microbial communities showed significant correlations with protein, lignin, and cellulose.					
35839908	7	9	with	correlations	954:965	arg1	protein					972:978	protein	972:978	protein	972:978	Microbial communities showed significant correlations with protein, lignin, and cellulose.					
35839908	7	9	with	correlations	954:965	arg1	lignin					981:986	lignin	981:986	lignin	981:986	Microbial communities showed significant correlations with protein, lignin, and cellulose.					
35839908	5	10	theme	pectin	691:696	arg1	contents					662:669	contents	662:669	contents of protein, starch, pectin, lignin, and cellulose	662:719	The results showed that contents of protein, starch, pectin, lignin, and cellulose varied in fermented leaves from different growing sites.					
35839908	9	11	theme	hydrolytic	1239:1248	arg1	enzymes					1250:1256	relating hydrolytic enzymes	1230:1256	relating hydrolytic enzymes	1230:1256	Leaf20) were identified and relating hydrolytic enzymes were annotated.					
35839908	11	12	from	fermentation	1608:1619	arg1	understanding					1566:1578	the understanding	1562:1578	the understanding of microbial roles in plant fermentation	1562:1619	Our work should deepen the understanding of microbial roles in plant fermentation and provide a new viewpoint for applying microbial consortia to convert plant organic components to small molecules.					
35839908	5	13	theme	different	753:761	arg1	sites					771:775	different growing sites	753:775	different growing sites	753:775	The results showed that contents of protein, starch, pectin, lignin, and cellulose varied in fermented leaves from different growing sites.					
35839908	8	14	theme	lignin	1095:1100	arg1	degradations					1022:1033	degradations	1022:1033	degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp	1022:1199	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
35839908	6	15	theme	taxonomic	834:842	arg1	composition					844:854	taxonomic composition	834:854	taxonomic composition	834:854	The different compound contents were closely related to taxonomic composition and functional profiles of foliar microbial communities.					
35839908	10	16	theme	cellulolytic	1380:1391	arg1	genomes					1393:1399	six potential cellulolytic genomes	1366:1399	six potential cellulolytic genomes	1366:1399	Further, twenty-two metagenome-assembled genomes (MAGs) were assembled from metagenomes and six potential cellulolytic genomes were used to reconstruct the cellulose-degrading process, revealing the potential metabolic cooperation related to cellulose degradation.					
35839908	8	17	theme	Sphingomonas	1185:1196	arg1	sp					1198:1199	Sphingomonas sp	1185:1199	Sphingomonas sp	1185:1199	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
35839908	4	18	used	used	436:439	arg2	we					433:434	we	433:434	we	433:434	In this study, we used a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in compound degradation and explore the potential synergy among community members in fermented tobacco leaves.					
35839908	11	19	from	understanding	1566:1578	arg1	fermentation					1608:1619	plant fermentation	1602:1619	plant fermentation	1602:1619	Our work should deepen the understanding of microbial roles in plant fermentation and provide a new viewpoint for applying microbial consortia to convert plant organic components to small molecules.					
35839908	7	20	theme	Microbial	913:921	arg1	communities					923:933	Microbial communities	913:933	Microbial communities	913:933	Microbial communities showed significant correlations with protein, lignin, and cellulose.					
35839908	0	21	from	degradation	39:49	arg1	plant					85:89	fermented plant	75:89	fermented plant	75:89	Metagenomic insight into the microbial degradation of organic compounds in fermented plant leaves.					
35839908	8	22	theme	Klebsiella	1103:1112	arg1	pneumoniae					1114:1123	Klebsiella pneumoniae	1103:1123	Klebsiella pneumoniae	1103:1123	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
35839908	2	23	theme	microorganisms	284:297	arg1	actions					268:274	enzymatic actions	258:274	enzymatic actions of many microorganisms	258:297	Fermentation of plant leaves involves enzymatic actions of many microorganisms.					
35839908	1	24	theme	efficient	182:190	arg1	part					192:195	an environmentally benign and energy efficient part	145:195	an environmentally benign and energy efficient part in product processing	145:217	Microbial degradation of organic compounds is an environmentally benign and energy efficient part in product processing.					
35839908	1	24	theme	efficient	182:190	arg1	degradation					109:119	Microbial degradation	99:119	Microbial degradation of organic compounds	99:140	Microbial degradation of organic compounds is an environmentally benign and energy efficient part in product processing.					
35839908	0	25	theme	fermented	75:83	arg1	plant					85:89	fermented plant	75:89	fermented plant	75:89	Metagenomic insight into the microbial degradation of organic compounds in fermented plant leaves.					
35839908	5	26	theme	protein	674:680	arg1	contents					662:669	contents	662:669	contents of protein, starch, pectin, lignin, and cellulose	662:719	The results showed that contents of protein, starch, pectin, lignin, and cellulose varied in fermented leaves from different growing sites.					
35839908	5	27	dep	fermented	731:739	arg1	leaves					741:746	leaves	741:746	leaves	741:746	The results showed that contents of protein, starch, pectin, lignin, and cellulose varied in fermented leaves from different growing sites.					
35839908	11	28	theme	organic	1699:1705	arg1	components					1707:1716	plant organic components	1693:1716	plant organic components	1693:1716	Our work should deepen the understanding of microbial roles in plant fermentation and provide a new viewpoint for applying microbial consortia to convert plant organic components to small molecules.					
35839908	0	29	theme	Metagenomic	0:10	arg1	insight					12:18	Metagenomic insight	0:18	Metagenomic insight into the microbial degradation of organic compounds in fermented plant	0:89	Metagenomic insight into the microbial degradation of organic compounds in fermented plant leaves.					
35839908	4	30	theme	community	591:599	arg1	members					601:607	community members	591:607	community members	591:607	In this study, we used a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in compound degradation and explore the potential synergy among community members in fermented tobacco leaves.					
35839908	6	31	theme	functional	860:869	arg1	profiles					871:878	functional profiles	860:878	functional profiles	860:878	The different compound contents were closely related to taxonomic composition and functional profiles of foliar microbial communities.					
35839908	11	32	theme	microbial	1662:1670	arg1	consortia					1672:1680	microbial consortia	1662:1680	microbial consortia	1662:1680	Our work should deepen the understanding of microbial roles in plant fermentation and provide a new viewpoint for applying microbial consortia to convert plant organic components to small molecules.					
35839908	11	33	from	roles	1593:1597	arg1	fermentation					1608:1619	plant fermentation	1602:1619	plant fermentation	1602:1619	Our work should deepen the understanding of microbial roles in plant fermentation and provide a new viewpoint for applying microbial consortia to convert plant organic components to small molecules.					
35839908	3	34	theme	cultural	400:407	arg1	methods					409:415	cultural methods	400:415	cultural methods	400:415	However, microbes and enzymes discovered from natural degradation communities were still limited by cultural methods.					
35839908	10	35	theme	related	1505:1511	arg1	cooperation					1493:1503	the potential metabolic cooperation	1469:1503	the potential metabolic cooperation related to cellulose degradation	1469:1536	Further, twenty-two metagenome-assembled genomes (MAGs) were assembled from metagenomes and six potential cellulolytic genomes were used to reconstruct the cellulose-degrading process, revealing the potential metabolic cooperation related to cellulose degradation.					
35839908	1	36	theme	Microbial	99:107	arg1	part					192:195	an environmentally benign and energy efficient part	145:195	an environmentally benign and energy efficient part in product processing	145:217	Microbial degradation of organic compounds is an environmentally benign and energy efficient part in product processing.					
35839908	1	36	theme	Microbial	99:107	arg1	degradation					109:119	Microbial degradation	99:119	Microbial degradation of organic compounds	99:140	Microbial degradation of organic compounds is an environmentally benign and energy efficient part in product processing.					
35839908	9	37	theme	relating	1230:1237	arg1	enzymes					1250:1256	relating hydrolytic enzymes	1230:1256	relating hydrolytic enzymes	1230:1256	Leaf20) were identified and relating hydrolytic enzymes were annotated.					
35839908	2	38	dep	plant	236:240	arg1	leaves					242:247	leaves	242:247	leaves	242:247	Fermentation of plant leaves involves enzymatic actions of many microorganisms.					
35839908	8	39	theme	Vital	1004:1008	arg1	species					1010:1016	Vital species	1004:1016	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.	1004:1200	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
35839908	8	40	dep	protein	1038:1044	arg1	cereus					1056:1061	Bacillus cereus	1047:1061	Bacillus cereus	1047:1061	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
35839908	8	40	dep	protein	1038:1044	arg1	aidingensis					1081:1091	Terribacillus aidingensis	1067:1091	Terribacillus aidingensis	1067:1091	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
35839908	10	41	theme	cellulose	1516:1524	arg1	degradation					1526:1536	cellulose degradation	1516:1536	cellulose degradation	1516:1536	Further, twenty-two metagenome-assembled genomes (MAGs) were assembled from metagenomes and six potential cellulolytic genomes were used to reconstruct the cellulose-degrading process, revealing the potential metabolic cooperation related to cellulose degradation.					
35839908	4	42	from	synergy	577:583	arg1	tobacco					622:628	fermented tobacco leaves	612:635	fermented tobacco leaves	612:635	In this study, we used a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in compound degradation and explore the potential synergy among community members in fermented tobacco leaves.					
35839908	4	43	dep	microbes	497:504	arg1	the					493:495	the	493:495	the	493:495	In this study, we used a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in compound degradation and explore the potential synergy among community members in fermented tobacco leaves.					
35839908	8	44	theme	cellulose	1151:1159	arg1	degradations					1022:1033	degradations	1022:1033	degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp	1022:1199	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
35839908	10	45	theme	potential	1370:1378	arg1	genomes					1393:1399	six potential cellulolytic genomes	1366:1399	six potential cellulolytic genomes	1366:1399	Further, twenty-two metagenome-assembled genomes (MAGs) were assembled from metagenomes and six potential cellulolytic genomes were used to reconstruct the cellulose-degrading process, revealing the potential metabolic cooperation related to cellulose degradation.					
35839908	0	46	theme	microbial	29:37	arg1	degradation					39:49	the microbial degradation	25:49	the microbial degradation of organic compounds in fermented plant	25:89	Metagenomic insight into the microbial degradation of organic compounds in fermented plant leaves.					
35839908	4	47	dep	tobacco	622:628	arg1	leaves					630:635	leaves	630:635	leaves	630:635	In this study, we used a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in compound degradation and explore the potential synergy among community members in fermented tobacco leaves.					
35839908	6	48	theme	compound	792:799	arg1	contents					801:808	The different compound contents	778:808	The different compound contents	778:808	The different compound contents were closely related to taxonomic composition and functional profiles of foliar microbial communities.					
35839908	6	48	theme	compound	792:799	arg1	related					823:829	related	823:829	related	823:829	The different compound contents were closely related to taxonomic composition and functional profiles of foliar microbial communities.					
35839908	0	49	theme	organic	54:60	arg1	compounds					62:70	organic compounds	54:70	organic compounds	54:70	Metagenomic insight into the microbial degradation of organic compounds in fermented plant leaves.					
35839908	5	50	theme	lignin	699:704	arg1	contents					662:669	contents	662:669	contents of protein, starch, pectin, lignin, and cellulose	662:719	The results showed that contents of protein, starch, pectin, lignin, and cellulose varied in fermented leaves from different growing sites.					
35839908	6	51	theme	microbial	890:898	arg1	communities					900:910	foliar microbial communities	883:910	foliar microbial communities	883:910	The different compound contents were closely related to taxonomic composition and functional profiles of foliar microbial communities.					
35839908	2	52	theme	plant	236:240	arg1	Fermentation					220:231	Fermentation	220:231	Fermentation of plant leaves	220:247	Fermentation of plant leaves involves enzymatic actions of many microorganisms.					
35839908	6	53	theme	different	782:790	arg1	contents					801:808	The different compound contents	778:808	The different compound contents	778:808	The different compound contents were closely related to taxonomic composition and functional profiles of foliar microbial communities.					
35839908	6	53	theme	different	782:790	arg1	related					823:829	related	823:829	related	823:829	The different compound contents were closely related to taxonomic composition and functional profiles of foliar microbial communities.					
35839908	6	54	theme	foliar	883:888	arg1	communities					900:910	foliar microbial communities	883:910	foliar microbial communities	883:910	The different compound contents were closely related to taxonomic composition and functional profiles of foliar microbial communities.					
35839908	11	55	theme	plant	1693:1697	arg1	components					1707:1716	plant organic components	1693:1716	plant organic components	1693:1716	Our work should deepen the understanding of microbial roles in plant fermentation and provide a new viewpoint for applying microbial consortia to convert plant organic components to small molecules.					
35839908	1	56	theme	organic	124:130	arg1	compounds					132:140	organic compounds	124:140	organic compounds	124:140	Microbial degradation of organic compounds is an environmentally benign and energy efficient part in product processing.					
35839908	8	57	dep	degradations	1022:1033	arg1	putida					1174:1179	Pseudomonas putida	1162:1179	Pseudomonas putida	1162:1179	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
35839908	8	57	dep	degradations	1022:1033	arg1	sp					1198:1199	Sphingomonas sp	1185:1199	Sphingomonas sp	1185:1199	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
35839908	4	58	theme	potential	567:575	arg1	synergy					577:583	the potential synergy	563:583	the potential synergy among community members in fermented tobacco leaves	563:635	In this study, we used a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in compound degradation and explore the potential synergy among community members in fermented tobacco leaves.					
35839908	2	59	theme	many	279:282	arg1	microorganisms					284:297	many microorganisms	279:297	many microorganisms	279:297	Fermentation of plant leaves involves enzymatic actions of many microorganisms.					
35839908	10	60	theme	metabolic	1483:1491	arg1	cooperation					1493:1503	the potential metabolic cooperation	1469:1503	the potential metabolic cooperation related to cellulose degradation	1469:1536	Further, twenty-two metagenome-assembled genomes (MAGs) were assembled from metagenomes and six potential cellulolytic genomes were used to reconstruct the cellulose-degrading process, revealing the potential metabolic cooperation related to cellulose degradation.					
35839908	1	61	theme	compounds	132:140	arg1	part					192:195	an environmentally benign and energy efficient part	145:195	an environmentally benign and energy efficient part in product processing	145:217	Microbial degradation of organic compounds is an environmentally benign and energy efficient part in product processing.					
35839908	1	61	theme	compounds	132:140	arg1	degradation					109:119	Microbial degradation	99:119	Microbial degradation of organic compounds	99:140	Microbial degradation of organic compounds is an environmentally benign and energy efficient part in product processing.					
35839908	11	62	theme	plant	1602:1606	arg1	fermentation					1608:1619	plant fermentation	1602:1619	plant fermentation	1602:1619	Our work should deepen the understanding of microbial roles in plant fermentation and provide a new viewpoint for applying microbial consortia to convert plant organic components to small molecules.					
35839908	5	63	theme	growing	763:769	arg1	sites					771:775	different growing sites	753:775	different growing sites	753:775	The results showed that contents of protein, starch, pectin, lignin, and cellulose varied in fermented leaves from different growing sites.					
35839908	5	64	theme	cellulose	711:719	arg1	contents					662:669	contents	662:669	contents of protein, starch, pectin, lignin, and cellulose	662:719	The results showed that contents of protein, starch, pectin, lignin, and cellulose varied in fermented leaves from different growing sites.					
35839908	4	65	theme	sequence-guided	456:470	arg1	strategy					472:479	a metagenomics sequence-guided strategy	441:479	a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in compound degradation and explore the potential synergy among community members in fermented tobacco leaves	441:635	In this study, we used a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in compound degradation and explore the potential synergy among community members in fermented tobacco leaves.					
35839908	1	66	from	part	192:195	arg1	processing					208:217	product processing	200:217	product processing	200:217	Microbial degradation of organic compounds is an environmentally benign and energy efficient part in product processing.					
35839908	11	67	theme	new	1635:1637	arg1	viewpoint					1639:1647	a new viewpoint	1633:1647	a new viewpoint for applying microbial consortia to convert plant organic components to small molecules	1633:1735	Our work should deepen the understanding of microbial roles in plant fermentation and provide a new viewpoint for applying microbial consortia to convert plant organic components to small molecules.					
35839908	10	68	theme	metagenome-assembled	1294:1313	arg1	genomes					1315:1321	twenty-two metagenome-assembled genomes	1283:1321	twenty-two metagenome-assembled genomes (MAGs)	1283:1328	Further, twenty-two metagenome-assembled genomes (MAGs) were assembled from metagenomes and six potential cellulolytic genomes were used to reconstruct the cellulose-degrading process, revealing the potential metabolic cooperation related to cellulose degradation.					
35839908	10	68	theme	metagenome-assembled	1294:1313	arg1	MAGs					1324:1327	MAGs	1324:1327	MAGs	1324:1327	Further, twenty-two metagenome-assembled genomes (MAGs) were assembled from metagenomes and six potential cellulolytic genomes were used to reconstruct the cellulose-degrading process, revealing the potential metabolic cooperation related to cellulose degradation.					
35839908	11	69	theme	microbial	1583:1591	arg1	roles					1593:1597	microbial roles	1583:1597	microbial roles in plant fermentation	1583:1619	Our work should deepen the understanding of microbial roles in plant fermentation and provide a new viewpoint for applying microbial consortia to convert plant organic components to small molecules.					
35839908	3	70	theme	degradation	354:364	arg1	communities					366:376	natural degradation communities	346:376	natural degradation communities	346:376	However, microbes and enzymes discovered from natural degradation communities were still limited by cultural methods.					
35839908	4	71	theme	metagenomics	443:454	arg1	strategy					472:479	a metagenomics sequence-guided strategy	441:479	a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in compound degradation and explore the potential synergy among community members in fermented tobacco leaves	441:635	In this study, we used a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in compound degradation and explore the potential synergy among community members in fermented tobacco leaves.					
35839908	1	72	theme	product	200:206	arg1	processing					208:217	product processing	200:217	product processing	200:217	Microbial degradation of organic compounds is an environmentally benign and energy efficient part in product processing.					
35839908	10	73	theme	potential	1473:1481	arg1	cooperation					1493:1503	the potential metabolic cooperation	1469:1503	the potential metabolic cooperation related to cellulose degradation	1469:1536	Further, twenty-two metagenome-assembled genomes (MAGs) were assembled from metagenomes and six potential cellulolytic genomes were used to reconstruct the cellulose-degrading process, revealing the potential metabolic cooperation related to cellulose degradation.					
35839908	11	74	theme	roles	1593:1597	arg1	understanding					1566:1578	the understanding	1562:1578	the understanding of microbial roles in plant fermentation	1562:1619	Our work should deepen the understanding of microbial roles in plant fermentation and provide a new viewpoint for applying microbial consortia to convert plant organic components to small molecules.					
35839908	0	75	theme	compounds	62:70	arg1	degradation					39:49	the microbial degradation	25:49	the microbial degradation of organic compounds in fermented plant	25:89	Metagenomic insight into the microbial degradation of organic compounds in fermented plant leaves.					
35839908	4	76	theme	compound	530:537	arg1	degradation					539:549	compound degradation	530:549	compound degradation	530:549	In this study, we used a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in compound degradation and explore the potential synergy among community members in fermented tobacco leaves.					
35839908	2	77	theme	enzymatic	258:266	arg1	actions					268:274	enzymatic actions	258:274	enzymatic actions of many microorganisms	258:297	Fermentation of plant leaves involves enzymatic actions of many microorganisms.					
35839908	11	78	theme	small	1721:1725	arg1	molecules					1727:1735	small molecules	1721:1735	small molecules	1721:1735	Our work should deepen the understanding of microbial roles in plant fermentation and provide a new viewpoint for applying microbial consortia to convert plant organic components to small molecules.					
35839908	7	79	theme	significant	942:952	arg1	correlations					954:965	significant correlations	942:965	significant correlations with protein, lignin, and cellulose	942:1001	Microbial communities showed significant correlations with protein, lignin, and cellulose.					
35839908	8	80	dep	lignin	1095:1100	arg1	pneumoniae					1114:1123	Klebsiella pneumoniae	1103:1123	Klebsiella pneumoniae	1103:1123	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
35839908	8	80	dep	lignin	1095:1100	arg1	ananatis					1137:1144	Pantoea ananatis	1129:1144	Pantoea ananatis	1129:1144	Vital species for degradations of protein (Bacillus cereus and Terribacillus aidingensis), lignin (Klebsiella pneumoniae and Pantoea ananatis) and cellulose (Pseudomonas putida and Sphingomonas sp.					
36395618	1	0	with	implants	153:160	arg1	degradation					180:190	controllable degradation	167:190	controllable degradation	167:190	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities has become a hotspot in the research field of biodegradable metallic materials.					
36395618	1	0	with	implants	153:160	arg1	capabilities					210:221	antibacterial capabilities	196:221	antibacterial capabilities	196:221	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities has become a hotspot in the research field of biodegradable metallic materials.					
36395618	1	1	theme	implants	153:160	arg1	development					106:116	The development	102:116	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities	102:221	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities has become a hotspot in the research field of biodegradable metallic materials.					
36395618	3	2	theme	high	647:650	arg1	hydrophobicity					663:676	hydrophobicity	663:676	hydrophobicity	663:676	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	3	2	theme	high	647:650	arg1	roughness					652:660	a high roughness	645:660	a high roughness	645:660	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	3	2	theme	high	647:650	arg1	resistance					697:706	fair corrosion resistance	682:706	fair corrosion resistance	682:706	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	2	3	contain	containing	394:403	arg2	Van					444:446	Van	444:446	Van	444:446	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	2	3	contain	containing	394:403	arg2	BNNS					435:438	BNNS	435:438	BNNS	435:438	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	2	3	contain	containing	394:403	arg2	components					423:432	two antibacterial components	405:432	two antibacterial components (BNNS and Van)	405:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	2	3	contain	containing	394:403	arg1	coating					386:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating	316:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van)	316:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	2	4	theme	_vancomycin	339:349	arg1	coating					386:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating	316:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van)	316:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	0	5	theme	orthopedic	77:86	arg1	applications					88:99	orthopedic applications	77:99	orthopedic applications	77:99	The enhanced antibacterial effect of BNNS_Van@CS/MAO coating on Mg alloy for orthopedic applications.					
36395618	4	6	theme	coli	848:851	arg1	growth					830:835	the growth	826:835	the growth of both E. coli and S. aureus	826:865	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	5	7	theme	composite	916:924	arg1	coating					926:932	the BNNS_Van@CS/MAO composite coating	896:932	the BNNS_Van@CS/MAO composite coating	896:932	The antibacterial effect of the BNNS_Van@CS/MAO composite coating was attributed to the synergistic effect of CS, BNNS and Van.					
36395618	1	8	theme	biodegradable	269:281	arg1	materials					292:300	biodegradable metallic materials	269:300	biodegradable metallic materials	269:300	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities has become a hotspot in the research field of biodegradable metallic materials.					
36395618	4	9	theme	In	709:710	arg1	experiments					732:742	In vitro antibacterial experiments	709:742	In vitro antibacterial experiments	709:742	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	3	10	theme	corrosion	687:695	arg1	roughness					652:660	a high roughness	645:660	a high roughness	645:660	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	3	10	theme	corrosion	687:695	arg1	resistance					697:706	fair corrosion resistance	682:706	fair corrosion resistance	682:706	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	2	11	theme	following	535:543	arg1	electrodeposition					545:561	following electrodeposition	535:561	following electrodeposition	535:561	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	5	12	theme	coating	926:932	arg1	effect					886:891	The antibacterial effect	868:891	The antibacterial effect of the BNNS_Van@CS/MAO composite coating	868:932	The antibacterial effect of the BNNS_Van@CS/MAO composite coating was attributed to the synergistic effect of CS, BNNS and Van.					
36395618	1	13	theme	metallic	283:290	arg1	materials					292:300	biodegradable metallic materials	269:300	biodegradable metallic materials	269:300	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities has become a hotspot in the research field of biodegradable metallic materials.					
36395618	6	14	theme	modification	1035:1046	arg1	strategy					1048:1055	a valuable surface modification strategy	1016:1055	a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties	1016:1164	This study provides a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties.					
36395618	6	15	theme	antibacterial	1141:1153	arg1	properties					1155:1164	antibacterial properties	1141:1164	antibacterial properties	1141:1164	This study provides a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties.					
36395618	1	16	theme	controllable	167:178	arg1	degradation					180:190	controllable degradation	167:190	controllable degradation	167:190	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities has become a hotspot in the research field of biodegradable metallic materials.					
36395618	2	17	theme	Mg	465:466	arg1	alloys					468:473	Mg alloys	465:473	Mg alloys	465:473	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	4	18	theme	composite	774:782	arg1	coating					784:790	the BNNS_Van@CS/MAO composite coating	754:790	the BNNS_Van@CS/MAO composite coating	754:790	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	6	19	theme	good	1111:1114	arg1	resistance					1126:1135	good corrosion resistance	1111:1135	good corrosion resistance	1111:1135	This study provides a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties.					
36395618	2	20	theme	nanosheets	321:330	arg1	coating					386:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating	316:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van)	316:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	6	21	theme	surface	1027:1033	arg1	strategy					1048:1055	a valuable surface modification strategy	1016:1055	a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties	1016:1164	This study provides a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties.					
36395618	2	22	theme	chitosan	358:365	arg1	coating					386:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating	316:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van)	316:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	0	23	theme	enhanced	4:11	arg1	effect					27:32	The enhanced antibacterial effect	0:32	The enhanced antibacterial effect of BNNS_Van@CS/MAO coating on Mg	0:65	The enhanced antibacterial effect of BNNS_Van@CS/MAO coating on Mg alloy for orthopedic applications.					
36395618	3	24	theme	fair	682:685	arg1	roughness					652:660	a high roughness	645:660	a high roughness	645:660	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	3	24	theme	fair	682:685	arg1	resistance					697:706	fair corrosion resistance	682:706	fair corrosion resistance	682:706	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	2	25	dep	components	423:432	arg1	BNNS					435:438	BNNS	435:438	BNNS	435:438	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	2	25	dep	components	423:432	arg1	components					423:432	two antibacterial components	405:432	two antibacterial components (BNNS and Van)	405:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	2	25	dep	components	423:432	arg1	Van					444:446	Van	444:446	Van	444:446	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	5	26	theme	synergistic	956:966	arg1	effect					968:973	the synergistic effect	952:973	the synergistic effect of CS, BNNS and Van	952:993	The antibacterial effect of the BNNS_Van@CS/MAO composite coating was attributed to the synergistic effect of CS, BNNS and Van.					
36395618	2	27	theme	BN	318:319	arg1	coating					386:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating	316:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van)	316:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	2	28	theme	@	357:357	arg1	coating					386:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating	316:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van)	316:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	2	29	theme	antibacterial	409:421	arg1	BNNS					435:438	BNNS	435:438	BNNS	435:438	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	2	29	theme	antibacterial	409:421	arg1	components					423:432	two antibacterial components	405:432	two antibacterial components (BNNS and Van)	405:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	2	29	theme	antibacterial	409:421	arg1	Van					444:446	Van	444:446	Van	444:446	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	1	30	theme	materials	292:300	arg1	field					260:264	the research field	247:264	the research field of biodegradable metallic materials	247:300	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities has become a hotspot in the research field of biodegradable metallic materials.					
36395618	5	31	theme	BNNS_Van	900:907	arg1	coating					926:932	the BNNS_Van@CS/MAO composite coating	896:932	the BNNS_Van@CS/MAO composite coating	896:932	The antibacterial effect of the BNNS_Van@CS/MAO composite coating was attributed to the synergistic effect of CS, BNNS and Van.					
36395618	1	32	theme	antibacterial	196:208	arg1	capabilities					210:221	antibacterial capabilities	196:221	antibacterial capabilities	196:221	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities has become a hotspot in the research field of biodegradable metallic materials.					
36395618	2	33	theme	Van	352:354	arg1	coating					386:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating	316:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van)	316:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	2	34	theme	MAO	502:504	arg1	pre-treatment					507:519	a micro-arc oxidation (MAO) pre-treatment	479:519	a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition	479:561	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	4	35	theme	antibacterial	718:730	arg1	experiments					732:742	In vitro antibacterial experiments	709:742	In vitro antibacterial experiments	709:742	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	6	36	with	implants	1097:1104	arg1	resistance					1126:1135	good corrosion resistance	1111:1135	good corrosion resistance	1111:1135	This study provides a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties.					
36395618	6	36	with	implants	1097:1104	arg1	properties					1155:1164	antibacterial properties	1141:1164	antibacterial properties	1141:1164	This study provides a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties.					
36395618	0	37	theme	antibacterial	13:25	arg1	effect					27:32	The enhanced antibacterial effect	0:32	The enhanced antibacterial effect of BNNS_Van@CS/MAO coating on Mg	0:65	The enhanced antibacterial effect of BNNS_Van@CS/MAO coating on Mg alloy for orthopedic applications.					
36395618	5	38	theme	CS/MAO	909:914	arg1	coating					926:932	the BNNS_Van@CS/MAO composite coating	896:932	the BNNS_Van@CS/MAO composite coating	896:932	The antibacterial effect of the BNNS_Van@CS/MAO composite coating was attributed to the synergistic effect of CS, BNNS and Van.					
36395618	2	39	theme	nanocomposite	372:384	arg1	coating					386:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating	316:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van)	316:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	0	40	theme	BNNS_Van	37:44	arg1	coating					53:59	BNNS_Van@CS/MAO coating	37:59	BNNS_Van@CS/MAO coating	37:59	The enhanced antibacterial effect of BNNS_Van@CS/MAO coating on Mg alloy for orthopedic applications.					
36395618	5	41	theme	antibacterial	872:884	arg1	effect					886:891	The antibacterial effect	868:891	The antibacterial effect of the BNNS_Van@CS/MAO composite coating	868:932	The antibacterial effect of the BNNS_Van@CS/MAO composite coating was attributed to the synergistic effect of CS, BNNS and Van.					
36395618	2	42	theme	oxidation	491:499	arg1	pre-treatment					507:519	a micro-arc oxidation (MAO) pre-treatment	479:519	a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition	479:561	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	6	43	theme	Mg-based	1088:1095	arg1	implants					1097:1104	multifunctional Mg-based implants	1072:1104	multifunctional Mg-based implants with good corrosion resistance and antibacterial properties	1072:1164	This study provides a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties.					
36395618	2	44	theme	 	356:356	arg1	coating					386:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating	316:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van)	316:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	6	45	theme	valuable	1018:1025	arg1	strategy					1048:1055	a valuable surface modification strategy	1016:1055	a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties	1016:1164	This study provides a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties.					
36395618	2	46	theme	micro-arc	481:489	arg1	pre-treatment					507:519	a micro-arc oxidation (MAO) pre-treatment	479:519	a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition	479:561	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	3	47	theme	coating	601:607	arg1	characterizations					576:592	The related characterizations	564:592	The related characterizations of the coating	564:607	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	5	48	theme	CS	978:979	arg1	effect					968:973	the synergistic effect	952:973	the synergistic effect of CS, BNNS and Van	952:993	The antibacterial effect of the BNNS_Van@CS/MAO composite coating was attributed to the synergistic effect of CS, BNNS and Van.					
36395618	2	49	theme	CS	368:369	arg1	coating					386:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating	316:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van)	316:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	0	50	theme	CS/MAO	46:51	arg1	coating					53:59	BNNS_Van@CS/MAO coating	37:59	BNNS_Van@CS/MAO coating	37:59	The enhanced antibacterial effect of BNNS_Van@CS/MAO coating on Mg alloy for orthopedic applications.					
36395618	4	51	theme	inhibitory	805:814	arg1	effect					816:821	obvious inhibitory effect	797:821	obvious inhibitory effect	797:821	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	0	52	theme	@	45:45	arg1	coating					53:59	BNNS_Van@CS/MAO coating	37:59	BNNS_Van@CS/MAO coating	37:59	The enhanced antibacterial effect of BNNS_Van@CS/MAO coating on Mg alloy for orthopedic applications.					
36395618	4	53	theme	obvious	797:803	arg1	effect					816:821	obvious inhibitory effect	797:821	obvious inhibitory effect	797:821	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	2	54	theme	BNNS	333:336	arg1	coating					386:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating	316:392	a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van)	316:447	To this end, a BN nanosheets (BNNS) _vancomycin (Van) @chitosan (CS) nanocomposite coating containing two antibacterial components (BNNS and Van) was prepared on Mg alloys via a micro-arc oxidation (MAO) pre-treatment combined with following electrodeposition.					
36395618	4	55	theme	@	766:766	arg1	coating					784:790	the BNNS_Van@CS/MAO composite coating	754:790	the BNNS_Van@CS/MAO composite coating	754:790	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	3	56	contain	has	641:643	arg2	resistance					697:706	fair corrosion resistance	682:706	fair corrosion resistance	682:706	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	3	56	contain	has	641:643	arg1	coating					633:639	the composite coating	619:639	the composite coating	619:639	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	3	56	contain	has	641:643	arg2	hydrophobicity					663:676	hydrophobicity	663:676	hydrophobicity	663:676	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	3	56	contain	has	641:643	arg2	roughness					652:660	a high roughness	645:660	a high roughness	645:660	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	4	57	dep	In	709:710	arg1	vitro					712:716	vitro	712:716	vitro	712:716	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	5	58	theme	@	908:908	arg1	coating					926:932	the BNNS_Van@CS/MAO composite coating	896:932	the BNNS_Van@CS/MAO composite coating	896:932	The antibacterial effect of the BNNS_Van@CS/MAO composite coating was attributed to the synergistic effect of CS, BNNS and Van.					
36395618	4	59	theme	aureus	860:865	arg1	growth					830:835	the growth	826:835	the growth of both E. coli and S. aureus	826:865	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	3	60	theme	composite	623:631	arg1	coating					633:639	the composite coating	619:639	the composite coating	619:639	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	4	61	theme	BNNS_Van	758:765	arg1	coating					784:790	the BNNS_Van@CS/MAO composite coating	754:790	the BNNS_Van@CS/MAO composite coating	754:790	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	4	62	contain	have	792:795	arg2	effect					816:821	obvious inhibitory effect	797:821	obvious inhibitory effect	797:821	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	4	62	contain	have	792:795	arg1	coating					784:790	the BNNS_Van@CS/MAO composite coating	754:790	the BNNS_Van@CS/MAO composite coating	754:790	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	1	63	theme	multifunctional	121:135	arg1	implants					153:160	multifunctional Mg-based active implants	121:160	multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities	121:221	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities has become a hotspot in the research field of biodegradable metallic materials.					
36395618	6	64	theme	multifunctional	1072:1086	arg1	implants					1097:1104	multifunctional Mg-based implants	1072:1104	multifunctional Mg-based implants with good corrosion resistance and antibacterial properties	1072:1164	This study provides a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties.					
36395618	3	65	theme	related	568:574	arg1	characterizations					576:592	The related characterizations	564:592	The related characterizations of the coating	564:607	The related characterizations of the coating show that the composite coating has a high roughness, hydrophobicity and fair corrosion resistance.					
36395618	1	66	theme	Mg-based	137:144	arg1	implants					153:160	multifunctional Mg-based active implants	121:160	multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities	121:221	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities has become a hotspot in the research field of biodegradable metallic materials.					
36395618	6	67	theme	corrosion	1116:1124	arg1	resistance					1126:1135	good corrosion resistance	1111:1135	good corrosion resistance	1111:1135	This study provides a valuable surface modification strategy for developing multifunctional Mg-based implants with good corrosion resistance and antibacterial properties.					
36395618	0	68	theme	coating	53:59	arg1	effect					27:32	The enhanced antibacterial effect	0:32	The enhanced antibacterial effect of BNNS_Van@CS/MAO coating on Mg	0:65	The enhanced antibacterial effect of BNNS_Van@CS/MAO coating on Mg alloy for orthopedic applications.					
36395618	0	69	from	effect	27:32	arg1	Mg					64:65	Mg	64:65	Mg	64:65	The enhanced antibacterial effect of BNNS_Van@CS/MAO coating on Mg alloy for orthopedic applications.					
36395618	4	70	theme	CS/MAO	767:772	arg1	coating					784:790	the BNNS_Van@CS/MAO composite coating	754:790	the BNNS_Van@CS/MAO composite coating	754:790	In vitro antibacterial experiments show that the BNNS_Van@CS/MAO composite coating have obvious inhibitory effect on the growth of both E. coli and S. aureus.					
36395618	1	71	theme	active	146:151	arg1	implants					153:160	multifunctional Mg-based active implants	121:160	multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities	121:221	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities has become a hotspot in the research field of biodegradable metallic materials.					
36395618	1	72	theme	research	251:258	arg1	field					260:264	the research field	247:264	the research field of biodegradable metallic materials	247:300	The development of multifunctional Mg-based active implants with controllable degradation and antibacterial capabilities has become a hotspot in the research field of biodegradable metallic materials.					
35467396	8	0	dep	reduction	1664:1672	arg1	ii					1660:1661	ii	1660:1661	ii	1660:1661	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	9	1	theme	bacterial	1983:1991	arg1	envelope					1998:2005	the bacterial cell envelope	1979:2005	the bacterial cell envelope	1979:2005	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	8	2	theme	surface	1603:1609	arg1	probing					1618:1624	(i) surface charge probing	1599:1624	(i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii)	1599:1734	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	5	3	theme	bacterial	1122:1130	arg1	CW					1132:1133	the bacterial CW	1118:1133	the bacterial CW	1118:1133	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	9	4	theme	binding	1895:1901	arg1	strength					1903:1910	binding strength	1895:1910	binding strength of SpM23 enzymes	1895:1927	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	11	5	from	environment	2389:2399	arg1	means					2334:2338	the means	2330:2338	the means of hydrolases activity regulation in the complex environment of the bacterial cell wall	2330:2426	We believe that our findings make an important contribution to understand the means of hydrolases activity regulation in the complex environment of the bacterial cell wall.					
35467396	10	6	theme	wall	2174:2177	arg1	components					2179:2188	charged cell wall components	2161:2188	charged cell wall components	2161:2188	Moreover, we revealed the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids, over the SpM23 enzymes.					
35467396	10	6	theme	wall	2174:2177	arg1	acids					2225:2229	teichoic and lipoteichoic acids	2199:2229	teichoic and lipoteichoic acids	2199:2229	Moreover, we revealed the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids, over the SpM23 enzymes.					
35467396	9	7	with	interactions	1961:1972	arg1	envelope					1998:2005	the bacterial cell envelope	1979:2005	the bacterial cell envelope	1979:2005	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	8	8	theme	bacterial	1629:1637	arg1	collection					1647:1656	collection	1647:1656	collection	1647:1656	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	8	8	theme	bacterial	1629:1637	arg1	strains					1639:1645	bacterial strains	1629:1645	bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii)	1629:1734	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	8	8	theme	bacterial	1629:1637	arg1	iii					1731:1733	iii	1731:1733	iii	1731:1733	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	10	9	theme	charged	2161:2167	arg1	components					2179:2188	charged cell wall components	2161:2188	charged cell wall components	2161:2188	Moreover, we revealed the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids, over the SpM23 enzymes.					
35467396	10	9	theme	charged	2161:2167	arg1	acids					2225:2229	teichoic and lipoteichoic acids	2199:2229	teichoic and lipoteichoic acids	2199:2229	Moreover, we revealed the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids, over the SpM23 enzymes.					
35467396	4	10	theme	cell	1060:1063	arg1	charge					1014:1019	the charge	1010:1019	the charge of both	1010:1027	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	4	10	theme	cell	1060:1063	arg1	surface					1065:1071	bacterial cell surface	1050:1071	bacterial cell surface	1050:1071	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	3	11	theme	same	700:703	arg1	host					715:718	the same bacterial host	696:718	the same bacterial host	696:718	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	11	12	from	regulation	2363:2372	arg1	environment					2389:2399	the complex environment	2377:2399	the complex environment of the bacterial cell wall	2377:2426	We believe that our findings make an important contribution to understand the means of hydrolases activity regulation in the complex environment of the bacterial cell wall.					
35467396	4	13	theme	both	1024:1027	arg1	molecule					1037:1044	enzyme molecule	1030:1044	enzyme molecule	1030:1044	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	4	13	theme	both	1024:1027	arg1	charge					1014:1019	the charge	1010:1019	the charge of both	1010:1027	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	4	13	theme	both	1024:1027	arg1	surface					1065:1071	bacterial cell surface	1050:1071	bacterial cell surface	1050:1071	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	8	14	theme	surface	1777:1783	arg1	charge					1753:1758	the net charge	1745:1758	the net charge of the bacterial surface	1745:1783	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	7	15	theme	cell	1564:1567	arg1	walls					1569:1573	bacterial cell walls	1554:1573	bacterial cell walls	1554:1573	IMPORTANCE This study shows direct relationship between the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls.					
35467396	2	16	theme	CW	526:527	arg1	surface					529:535	bacterial CW surface	516:535	bacterial CW surface	516:535	In the light of contradictory data reported, detailed mechanism of regulation of enzymes activity based on electrostatic interactions between hydrolase molecule and bacterial CW surface remains unknown.					
35467396	6	17	theme	hydrolases	1370:1379	arg1	development					1343:1353	the future development	1332:1353	the future development of chimeric PG hydrolases of desired antimicrobial specificity	1332:1416	These data serve as a hint for the future development of chimeric PG hydrolases of desired antimicrobial specificity.					
35467396	4	18	theme	enzyme	1030:1035	arg1	molecule					1037:1044	enzyme molecule	1030:1044	enzyme molecule	1030:1044	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	4	18	theme	enzyme	1030:1035	arg1	charge					1014:1019	the charge	1010:1019	the charge of both	1010:1027	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	3	19	theme	comprehensive	572:584	arg1	study					586:590	a comprehensive study	570:590	a comprehensive study	570:590	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	10	20	theme	components	2179:2188	arg1	role					2153:2156	the regulatory role	2138:2156	the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids	2138:2229	Moreover, we revealed the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids, over the SpM23 enzymes.					
35467396	11	21	from	means	2334:2338	arg1	environment					2389:2399	the complex environment	2377:2399	the complex environment of the bacterial cell wall	2377:2426	We believe that our findings make an important contribution to understand the means of hydrolases activity regulation in the complex environment of the bacterial cell wall.					
35467396	11	22	theme	complex	2381:2387	arg1	environment					2389:2399	the complex environment	2377:2399	the complex environment of the bacterial cell wall	2377:2426	We believe that our findings make an important contribution to understand the means of hydrolases activity regulation in the complex environment of the bacterial cell wall.					
35467396	1	23	theme	Peptidoglycan	123:135	arg1	hydrolases					142:151	Peptidoglycan (PG) hydrolases	123:151	Peptidoglycan (PG) hydrolases	123:151	Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.					
35467396	11	24	theme	wall	2423:2426	arg1	environment					2389:2399	the complex environment	2377:2399	the complex environment of the bacterial cell wall	2377:2426	We believe that our findings make an important contribution to understand the means of hydrolases activity regulation in the complex environment of the bacterial cell wall.					
35467396	5	25	theme	enzymes	1181:1187	arg1	regulation					1198:1207	enzymes activity regulation	1181:1207	enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases	1181:1298	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	8	26	theme	net	1681:1683	arg1	charge					1685:1690	the net charge	1677:1690	the net charge of the positively charged enzyme	1677:1723	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	7	27	theme	SpM23_A	1529:1535	arg1	charge					1487:1492	the surface charge	1475:1492	the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls	1475:1573	IMPORTANCE This study shows direct relationship between the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls.					
35467396	11	28	theme	bacterial	2408:2416	arg1	wall					2423:2426	the bacterial cell wall	2404:2426	the bacterial cell wall	2404:2426	We believe that our findings make an important contribution to understand the means of hydrolases activity regulation in the complex environment of the bacterial cell wall.					
35467396	5	29	theme	regulation	1198:1207	arg1	mechanism					1168:1176	the mechanism	1164:1176	the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases	1164:1298	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	3	30	theme	sequence	737:744	arg1	conservation					746:757	sequence conservation	737:757	sequence conservation	737:757	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	8	31	theme	teichoic	1829:1836	arg1	acids					1838:1842	teichoic acids	1829:1842	teichoic acids	1829:1842	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	7	32	theme	enzymes	1520:1526	arg1	charge					1487:1492	the surface charge	1475:1492	the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls	1475:1573	IMPORTANCE This study shows direct relationship between the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls.					
35467396	11	33	theme	important	2293:2301	arg1	contribution					2303:2314	an important contribution	2290:2314	an important contribution to understand the means of hydrolases activity regulation in the complex environment of the bacterial cell wall	2290:2426	We believe that our findings make an important contribution to understand the means of hydrolases activity regulation in the complex environment of the bacterial cell wall.					
35467396	1	34	theme	due	154:156	arg1	hydrolases					142:151	Peptidoglycan (PG) hydrolases	123:151	Peptidoglycan (PG) hydrolases	123:151	Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.					
35467396	3	35	theme	net	826:828	arg1	charges					830:836	surprisingly distinct net charges	804:836	surprisingly distinct net charges	804:836	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	3	36	from	similarities	721:732	arg1	conservation					746:757	sequence conservation	737:757	sequence conservation	737:757	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	3	36	from	similarities	721:732	arg1	architecture					767:778	domain architecture	760:778	domain architecture	760:778	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	3	36	from	similarities	721:732	arg1	structure					785:793	structure	785:793	structure	785:793	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	8	37	theme	enzyme	1718:1723	arg1	charge					1685:1690	the net charge	1677:1690	the net charge of the positively charged enzyme	1677:1723	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	2	38	theme	hydrolase	493:501	arg1	molecule					503:510	hydrolase molecule	493:510	hydrolase molecule	493:510	In the light of contradictory data reported, detailed mechanism of regulation of enzymes activity based on electrostatic interactions between hydrolase molecule and bacterial CW surface remains unknown.					
35467396	8	39	dep	strains	1639:1645	arg1	reduction					1664:1672	(ii) reduction	1659:1672	(ii) reduction of the net charge of the positively charged enzyme	1659:1723	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	8	39	dep	strains	1639:1645	arg1	collection					1647:1656	collection	1647:1656	collection	1647:1656	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	8	39	dep	strains	1639:1645	arg1	strains					1639:1645	bacterial strains	1629:1645	bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii)	1629:1734	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	8	39	dep	strains	1639:1645	arg1	iii					1731:1733	iii	1731:1733	iii	1731:1733	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	5	40	theme	charged	1255:1261	arg1	envelope					1273:1280	charged bacterial envelope	1255:1280	charged bacterial envelope	1255:1280	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	0	41	theme	Peptidoglycan	97:109	arg1	Hydrolases					111:120	Two Novel Peptidoglycan Hydrolases	87:120	Two Novel Peptidoglycan Hydrolases	87:120	Electrostatic Interaction with the Bacterial Cell Envelope Tunes the Lytic Activity of Two Novel Peptidoglycan Hydrolases.					
35467396	1	42	theme	conventional	325:336	arg1	antibiotics					338:348	conventional antibiotics	325:348	conventional antibiotics	325:348	Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.					
35467396	9	43	theme	susceptible	2085:2095	arg1	species					2107:2113	susceptible bacterial species	2085:2113	susceptible bacterial species	2085:2113	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	3	44	theme	2D	842:843	arg1	electrophoresis					845:859	2D electrophoresis	842:859	2D electrophoresis	842:859	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	4	45	theme	net	960:962	arg1	charge					964:969	hydrolases surface net charge	941:969	hydrolases surface net charge	941:969	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	11	46	theme	activity	2354:2361	arg1	regulation					2363:2372	hydrolases activity regulation	2343:2372	hydrolases activity regulation in the complex environment of the bacterial cell wall	2343:2426	We believe that our findings make an important contribution to understand the means of hydrolases activity regulation in the complex environment of the bacterial cell wall.					
35467396	0	47	theme	Lytic	69:73	arg1	Activity					75:82	the Lytic Activity	65:82	the Lytic Activity of Two Novel Peptidoglycan Hydrolases	65:120	Electrostatic Interaction with the Bacterial Cell Envelope Tunes the Lytic Activity of Two Novel Peptidoglycan Hydrolases.					
35467396	0	48	theme	Electrostatic	0:12	arg1	Interaction					14:24	Electrostatic Interaction	0:24	Electrostatic Interaction with the Bacterial Cell Envelope	0:57	Electrostatic Interaction with the Bacterial Cell Envelope Tunes the Lytic Activity of Two Novel Peptidoglycan Hydrolases.					
35467396	5	49	theme	anionic	1090:1096	arg1	acids					1083:1087	Teichoic acids	1074:1087	Teichoic acids	1074:1087	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	5	49	theme	anionic	1090:1096	arg1	polymers					1098:1105	anionic polymers	1090:1105	anionic polymers present in the bacterial CW	1090:1133	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	2	50	theme	detailed	396:403	arg1	mechanism					405:413	detailed mechanism	396:413	detailed mechanism of regulation of enzymes activity based on electrostatic interactions between hydrolase molecule and bacterial CW surface	396:535	In the light of contradictory data reported, detailed mechanism of regulation of enzymes activity based on electrostatic interactions between hydrolase molecule and bacterial CW surface remains unknown.					
35467396	6	51	theme	future	1336:1341	arg1	development					1343:1353	the future development	1332:1353	the future development of chimeric PG hydrolases of desired antimicrobial specificity	1332:1416	These data serve as a hint for the future development of chimeric PG hydrolases of desired antimicrobial specificity.					
35467396	5	52	theme	present	1107:1113	arg1	acids					1083:1087	Teichoic acids	1074:1087	Teichoic acids	1074:1087	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	5	52	theme	present	1107:1113	arg1	polymers					1098:1105	anionic polymers	1090:1105	anionic polymers present in the bacterial CW	1090:1133	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	2	53	theme	activity	440:447	arg1	regulation					418:427	regulation	418:427	regulation of enzymes activity	418:447	In the light of contradictory data reported, detailed mechanism of regulation of enzymes activity based on electrostatic interactions between hydrolase molecule and bacterial CW surface remains unknown.					
35467396	9	54	theme	enzymes	1921:1927	arg1	activity					1882:1889	lytic activity	1876:1889	lytic activity	1876:1889	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	9	54	theme	enzymes	1921:1927	arg1	strength					1903:1910	binding strength	1895:1910	binding strength of SpM23 enzymes	1895:1927	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	2	55	theme	contradictory	367:379	arg1	data					381:384	contradictory data	367:384	contradictory data reported	367:393	In the light of contradictory data reported, detailed mechanism of regulation of enzymes activity based on electrostatic interactions between hydrolase molecule and bacterial CW surface remains unknown.					
35467396	0	56	theme	Bacterial	35:43	arg1	Envelope					50:57	the Bacterial Cell Envelope	31:57	the Bacterial Cell Envelope	31:57	Electrostatic Interaction with the Bacterial Cell Envelope Tunes the Lytic Activity of Two Novel Peptidoglycan Hydrolases.					
35467396	1	57	theme	suitable	265:272	arg1	targets					274:280	suitable targets	265:280	suitable targets for therapies, and a potent alternative to conventional antibiotics	265:348	Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.					
35467396	4	58	theme	strong	914:919	arg1	correlation					921:931	a strong correlation	912:931	a strong correlation between hydrolases surface net charge and the enzymes activity	912:994	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	9	59	theme	bacterial	2097:2105	arg1	species					2107:2113	susceptible bacterial species	2085:2113	susceptible bacterial species	2085:2113	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	4	60	theme	hydrolases	941:950	arg1	charge					964:969	hydrolases surface net charge	941:969	hydrolases surface net charge	941:969	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	3	61	theme	novel	632:636	arg1	hydrolases					641:650	two novel PG hydrolases	628:650	a model two novel PG hydrolases	620:650	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	5	62	theme	PG	1286:1287	arg1	hydrolases					1289:1298	PG hydrolases	1286:1298	PG hydrolases	1286:1298	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	6	63	theme	antimicrobial	1392:1404	arg1	specificity					1406:1416	desired antimicrobial specificity	1384:1416	desired antimicrobial specificity	1384:1416	These data serve as a hint for the future development of chimeric PG hydrolases of desired antimicrobial specificity.					
35467396	1	64	theme	cell	215:218	arg1	CW					226:227	CW	226:227	CW	226:227	Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.					
35467396	1	64	theme	cell	215:218	arg1	wall					220:223	the bacterial cell wall	201:223	the bacterial cell wall (CW)	201:228	Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.					
35467396	4	65	theme	enzymes	979:985	arg1	activity					987:994	the enzymes activity	975:994	the enzymes activity	975:994	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	10	66	theme	regulatory	2142:2151	arg1	role					2153:2156	the regulatory role	2138:2156	the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids	2138:2229	Moreover, we revealed the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids, over the SpM23 enzymes.					
35467396	9	67	theme	cell	1993:1996	arg1	envelope					1998:2005	the bacterial cell envelope	1979:2005	the bacterial cell envelope	1979:2005	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	8	68	theme	net	1749:1751	arg1	charge					1753:1758	the net charge	1745:1758	the net charge of the bacterial surface	1745:1783	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	6	69	theme	PG	1367:1368	arg1	hydrolases					1370:1379	chimeric PG hydrolases	1358:1379	chimeric PG hydrolases of desired antimicrobial specificity	1358:1416	These data serve as a hint for the future development of chimeric PG hydrolases of desired antimicrobial specificity.					
35467396	4	70	theme	bacterial	1050:1058	arg1	charge					1014:1019	the charge	1010:1019	the charge of both	1010:1027	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	4	70	theme	bacterial	1050:1058	arg1	surface					1065:1071	bacterial cell surface	1050:1071	bacterial cell surface	1050:1071	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	5	71	from	present	1107:1113	arg1	CW					1132:1133	the bacterial CW	1118:1133	the bacterial CW	1118:1133	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	8	72	theme	charge	1611:1616	arg1	probing					1618:1624	(i) surface charge probing	1599:1624	(i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii)	1599:1734	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	10	73	theme	cell	2169:2172	arg1	components					2179:2188	charged cell wall components	2161:2188	charged cell wall components	2161:2188	Moreover, we revealed the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids, over the SpM23 enzymes.					
35467396	10	73	theme	cell	2169:2172	arg1	acids					2225:2229	teichoic and lipoteichoic acids	2199:2229	teichoic and lipoteichoic acids	2199:2229	Moreover, we revealed the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids, over the SpM23 enzymes.					
35467396	5	74	attach	present	1107:1113	arg1	CW					1132:1133	the bacterial CW	1118:1133	the bacterial CW	1118:1133	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	5	74	attach	present	1107:1113	arg2	polymers					1098:1105	anionic polymers	1090:1105	anionic polymers present in the bacterial CW	1090:1133	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	5	74	attach	present	1107:1113	arg2	acids					1083:1087	Teichoic acids	1074:1087	Teichoic acids	1074:1087	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	8	75	theme	bacterial	1767:1775	arg1	surface					1777:1783	the bacterial surface	1763:1783	the bacterial surface	1763:1783	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	1	76	theme	crucial	167:173	arg1	role					175:178	their crucial role	161:178	their crucial role in the metabolism of the bacterial cell wall (CW)	161:228	Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.					
35467396	7	77	theme	walls	1569:1573	arg1	charge					1487:1492	the surface charge	1475:1492	the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls	1475:1573	IMPORTANCE This study shows direct relationship between the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls.					
35467396	3	78	theme	bacterial	705:713	arg1	host					715:718	the same bacterial host	696:718	the same bacterial host	696:718	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	5	79	from	CW	1132:1133	arg1	present					1107:1113	present	1107:1113	present	1107:1113	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	8	80	theme	strains	1639:1645	arg1	probing					1618:1624	(i) surface charge probing	1599:1624	(i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii)	1599:1734	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	7	81	theme	bacterial	1554:1562	arg1	walls					1569:1573	bacterial cell walls	1554:1573	bacterial cell walls	1554:1573	IMPORTANCE This study shows direct relationship between the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls.					
35467396	2	82	theme	bacterial	516:524	arg1	surface					529:535	bacterial CW surface	516:535	bacterial CW surface	516:535	In the light of contradictory data reported, detailed mechanism of regulation of enzymes activity based on electrostatic interactions between hydrolase molecule and bacterial CW surface remains unknown.					
35467396	10	83	theme	lipoteichoic	2212:2223	arg1	components					2179:2188	charged cell wall components	2161:2188	charged cell wall components	2161:2188	Moreover, we revealed the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids, over the SpM23 enzymes.					
35467396	10	83	theme	lipoteichoic	2212:2223	arg1	acids					2225:2229	teichoic and lipoteichoic acids	2199:2229	teichoic and lipoteichoic acids	2199:2229	Moreover, we revealed the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids, over the SpM23 enzymes.					
35467396	11	84	theme	cell	2418:2421	arg1	wall					2423:2426	the bacterial cell wall	2404:2426	the bacterial cell wall	2404:2426	We believe that our findings make an important contribution to understand the means of hydrolases activity regulation in the complex environment of the bacterial cell wall.					
35467396	9	85	theme	spectrum	2073:2080	arg1	determination					2052:2064	the determination	2048:2064	the determination of the spectrum of susceptible bacterial species	2048:2113	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	3	86	theme	distinct	817:824	arg1	charges					830:836	surprisingly distinct net charges	804:836	surprisingly distinct net charges	804:836	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	10	87	theme	teichoic	2199:2206	arg1	components					2179:2188	charged cell wall components	2161:2188	charged cell wall components	2161:2188	Moreover, we revealed the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids, over the SpM23 enzymes.					
35467396	10	87	theme	teichoic	2199:2206	arg1	acids					2225:2229	teichoic and lipoteichoic acids	2199:2229	teichoic and lipoteichoic acids	2199:2229	Moreover, we revealed the regulatory role of charged cell wall components, namely, teichoic and lipoteichoic acids, over the SpM23 enzymes.					
35467396	5	88	theme	activity	1189:1196	arg1	regulation					1198:1207	enzymes activity regulation	1181:1207	enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases	1181:1298	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	3	89	theme	domain	760:765	arg1	architecture					767:778	domain architecture	760:778	domain architecture	760:778	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	8	90	theme	acids	1838:1842	arg1	composition					1814:1824	composition	1814:1824	composition	1814:1824	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	8	90	theme	acids	1838:1842	arg1	content					1802:1808	content	1802:1808	content	1802:1808	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	1	91	from	role	175:178	arg1	metabolism					187:196	the metabolism	183:196	the metabolism of the bacterial cell wall (CW)	183:228	Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.					
35467396	7	92	theme	described	1510:1518	arg1	enzymes					1520:1526	two recently described enzymes	1497:1526	two recently described enzymes	1497:1526	IMPORTANCE This study shows direct relationship between the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls.					
35467396	2	93	theme	electrostatic	458:470	arg1	interactions					472:483	electrostatic interactions	458:483	electrostatic interactions between hydrolase molecule	458:510	In the light of contradictory data reported, detailed mechanism of regulation of enzymes activity based on electrostatic interactions between hydrolase molecule and bacterial CW surface remains unknown.					
35467396	9	94	theme	species	2107:2113	arg1	spectrum					2073:2080	the spectrum	2069:2080	the spectrum of susceptible bacterial species	2069:2113	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	8	95	theme	charge	1685:1690	arg1	reduction					1664:1672	(ii) reduction	1659:1672	(ii) reduction of the net charge of the positively charged enzyme	1659:1723	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	8	95	theme	charge	1685:1690	arg1	collection					1647:1656	collection	1647:1656	collection	1647:1656	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	0	96	theme	Novel	91:95	arg1	Hydrolases					111:120	Two Novel Peptidoglycan Hydrolases	87:120	Two Novel Peptidoglycan Hydrolases	87:120	Electrostatic Interaction with the Bacterial Cell Envelope Tunes the Lytic Activity of Two Novel Peptidoglycan Hydrolases.					
35467396	1	97	theme	potent	303:308	arg1	alternative					310:320	a potent alternative	301:320	a potent alternative to conventional antibiotics	301:348	Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.					
35467396	3	98	dep	display	796:802	arg1	pI					862:863	pI 6.8	862:867	pI 6.8	862:867	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	3	98	dep	display	796:802	arg1	electrophoresis					845:859	2D electrophoresis	842:859	2D electrophoresis	842:859	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	3	98	dep	display	796:802	arg1	study					586:590	a comprehensive study	570:590	a comprehensive study	570:590	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	3	98	dep	display	796:802	arg1	pI					874:875	pI 9.7	874:879	pI 9.7	874:879	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	11	99	theme	regulation	2363:2372	arg1	means					2334:2338	the means	2330:2338	the means of hydrolases activity regulation in the complex environment of the bacterial cell wall	2330:2426	We believe that our findings make an important contribution to understand the means of hydrolases activity regulation in the complex environment of the bacterial cell wall.					
35467396	9	100	theme	lytic	1876:1880	arg1	activity					1882:1889	lytic activity	1876:1889	lytic activity	1876:1889	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	3	101	from	study	586:590	arg1	phenomenon					600:609	this phenomenon	595:609	this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B	595:672	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	0	102	theme	Hydrolases	111:120	arg1	Activity					75:82	the Lytic Activity	65:82	the Lytic Activity of Two Novel Peptidoglycan Hydrolases	65:120	Electrostatic Interaction with the Bacterial Cell Envelope Tunes the Lytic Activity of Two Novel Peptidoglycan Hydrolases.					
35467396	8	103	theme	charged	1710:1716	arg1	enzyme					1718:1723	the positively charged enzyme	1695:1723	the positively charged enzyme	1695:1723	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	11	104	theme	hydrolases	2343:2352	arg1	regulation					2363:2372	hydrolases activity regulation	2343:2372	hydrolases activity regulation in the complex environment of the bacterial cell wall	2343:2426	We believe that our findings make an important contribution to understand the means of hydrolases activity regulation in the complex environment of the bacterial cell wall.					
35467396	5	105	theme	bacterial	1263:1271	arg1	envelope					1273:1280	charged bacterial envelope	1255:1280	charged bacterial envelope	1255:1280	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	6	106	theme	chimeric	1358:1365	arg1	hydrolases					1370:1379	chimeric PG hydrolases	1358:1379	chimeric PG hydrolases of desired antimicrobial specificity	1358:1416	These data serve as a hint for the future development of chimeric PG hydrolases of desired antimicrobial specificity.					
35467396	4	107	theme	surface	952:958	arg1	charge					964:969	hydrolases surface net charge	941:969	hydrolases surface net charge	941:969	We demonstrate a strong correlation between hydrolases surface net charge and the enzymes activity by modulating the charge of both, enzyme molecule and bacterial cell surface.					
35467396	8	108	dep	probing	1618:1624	arg1	i					1600:1600	i	1600:1600	i	1600:1600	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	8	109	dep	content	1802:1808	arg1	the					1798:1800	the	1798:1800	the	1798:1800	We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.					
35467396	9	110	theme	SpM23	1915:1919	arg1	enzymes					1921:1927	SpM23 enzymes	1915:1927	SpM23 enzymes	1915:1927	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	5	111	theme	electrostatics-based	1216:1235	arg1	interplay					1237:1245	the electrostatics-based interplay	1212:1245	the electrostatics-based interplay between charged bacterial envelope and PG hydrolases	1212:1298	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	2	112	theme	data	381:384	arg1	light					358:362	the light	354:362	the light of contradictory data reported	354:393	In the light of contradictory data reported, detailed mechanism of regulation of enzymes activity based on electrostatic interactions between hydrolase molecule and bacterial CW surface remains unknown.					
35467396	0	113	theme	Cell	45:48	arg1	Envelope					50:57	the Bacterial Cell Envelope	31:57	the Bacterial Cell Envelope	31:57	Electrostatic Interaction with the Bacterial Cell Envelope Tunes the Lytic Activity of Two Novel Peptidoglycan Hydrolases.					
35467396	7	114	dep	IMPORTANCE	1419:1428	arg1	shows					1441:1445	shows	1441:1445	shows direct relationship between the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls	1441:1573	IMPORTANCE This study shows direct relationship between the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls.					
35467396	7	115	theme	surface	1479:1485	arg1	charge					1487:1492	the surface charge	1475:1492	the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls	1475:1573	IMPORTANCE This study shows direct relationship between the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls.					
35467396	2	116	theme	enzymes	432:438	arg1	activity					440:447	enzymes activity	432:447	enzymes activity	432:447	In the light of contradictory data reported, detailed mechanism of regulation of enzymes activity based on electrostatic interactions between hydrolase molecule and bacterial CW surface remains unknown.					
35467396	5	117	theme	Teichoic	1074:1081	arg1	acids					1083:1087	Teichoic acids	1074:1087	Teichoic acids	1074:1087	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	5	117	theme	Teichoic	1074:1081	arg1	polymers					1098:1105	anionic polymers	1090:1105	anionic polymers present in the bacterial CW	1090:1133	Teichoic acids, anionic polymers present in the bacterial CW, are shown to be involved in the mechanism of enzymes activity regulation by the electrostatics-based interplay between charged bacterial envelope and PG hydrolases.					
35467396	2	118	theme	regulation	418:427	arg1	mechanism					405:413	detailed mechanism	396:413	detailed mechanism of regulation of enzymes activity based on electrostatic interactions between hydrolase molecule and bacterial CW surface	396:535	In the light of contradictory data reported, detailed mechanism of regulation of enzymes activity based on electrostatic interactions between hydrolase molecule and bacterial CW surface remains unknown.					
35467396	3	119	theme	PG	638:639	arg1	hydrolases					641:650	two novel PG hydrolases	628:650	a model two novel PG hydrolases	620:650	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35467396	0	120	with	Interaction	14:24	arg1	Envelope					50:57	the Bacterial Cell Envelope	31:57	the Bacterial Cell Envelope	31:57	Electrostatic Interaction with the Bacterial Cell Envelope Tunes the Lytic Activity of Two Novel Peptidoglycan Hydrolases.					
35467396	7	121	theme	direct	1447:1452	arg1	relationship					1454:1465	direct relationship	1447:1465	direct relationship between the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls	1447:1573	IMPORTANCE This study shows direct relationship between the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls.					
35467396	9	122	theme	electrostatic	1947:1959	arg1	interactions					1961:1972	electrostatic interactions	1947:1972	electrostatic interactions with the bacterial cell envelope	1947:2005	In all cases, we observed that lytic activity and binding strength of SpM23 enzymes, are regulated by electrostatic interactions with the bacterial cell envelope and that this interaction contributes to the determination of the spectrum of susceptible bacterial species.					
35467396	6	123	theme	specificity	1406:1416	arg1	hydrolases					1370:1379	chimeric PG hydrolases	1358:1379	chimeric PG hydrolases of desired antimicrobial specificity	1358:1416	These data serve as a hint for the future development of chimeric PG hydrolases of desired antimicrobial specificity.					
35467396	1	124	theme	bacterial	205:213	arg1	CW					226:227	CW	226:227	CW	226:227	Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.					
35467396	1	124	theme	bacterial	205:213	arg1	wall					220:223	the bacterial cell wall	201:223	the bacterial cell wall (CW)	201:228	Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.					
35467396	1	125	theme	wall	220:223	arg1	metabolism					187:196	the metabolism	183:196	the metabolism of the bacterial cell wall (CW)	183:228	Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.					
35467396	6	126	theme	desired	1384:1390	arg1	specificity					1406:1416	desired antimicrobial specificity	1384:1416	desired antimicrobial specificity	1384:1416	These data serve as a hint for the future development of chimeric PG hydrolases of desired antimicrobial specificity.					
35467396	3	127	dep	model	622:626	arg1	hydrolases					641:650	two novel PG hydrolases	628:650	a model two novel PG hydrolases	620:650	Here, we report a comprehensive study on this phenomenon using as a model two novel PG hydrolases, SpM23_A, and SpM23_B, which although share the same bacterial host, similarities in sequence conservation, domain architecture, and structure, display surprisingly distinct net charges (in 2D electrophoresis, pI 6.8, and pI 9.7, respectively).					
35247663	3	0	theme	Higher	615:620	arg1	magnitudes					625:634	Higher kr magnitudes	615:634	Higher kr magnitudes	615:634	Higher kr magnitudes were found in WRS-based samples (88.2-12.8 × 10-3 day-1) than in counterparts prepared with TS (87.6-8.96 × 10-3 day-1).					
35247663	2	1	theme	kr	463:464	arg1	parameters					466:475	The R and kr parameters	453:475	The R and kr parameters	453:475	The R and kr parameters were affected by non-covalent interactions of ACs with biopolymers and the development of a compact structure induced by starch addition.					
35247663	2	2	theme	R	457:457	arg1	parameters					466:475	The R and kr parameters	453:475	The R and kr parameters	453:475	The R and kr parameters were affected by non-covalent interactions of ACs with biopolymers and the development of a compact structure induced by starch addition.					
35247663	4	3	theme	vaporization	791:802	arg1	enthalpies					804:813	vaporization enthalpies	791:813	vaporization enthalpies of ACs	791:820	Thermodynamic tests revealed that vaporization enthalpies of ACs were in ranges: 22.6-41.2 kJ mol-1 or 21.4-39.8 kJ mol-1 in samples containing WRS or TS, respectively.					
35247663	1	4	from	gels	353:356	arg1	2-heptanone					308:318	2-heptanone	308:318	2-heptanone	308:318	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	4	from	gels	353:356	arg1	1-octen-3-ol					294:305	1-octen-3-ol	294:305	1-octen-3-ol	294:305	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	4	from	gels	353:356	arg1	linalool					284:291	linalool	284:291	linalool	284:291	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	4	from	gels	353:356	arg1	butyrate					274:281	ethyl butyrate	268:281	ethyl butyrate	268:281	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	4	from	gels	353:356	arg1	octanal					324:330	octanal	324:330	octanal	324:330	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	0	5	from	Effects	0:6	arg1	release					103:109	release	103:109	release	103:109	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	0	5	from	Effects	0:6	arg1	retention					89:97	retention	89:97	retention	89:97	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	2	6	theme	non-covalent	494:505	arg1	interactions					507:518	non-covalent interactions	494:518	non-covalent interactions of ACs with biopolymers and the development of a compact structure induced by starch addition	494:612	The R and kr parameters were affected by non-covalent interactions of ACs with biopolymers and the development of a compact structure induced by starch addition.					
35247663	1	7	theme	myofibrillar	380:391	arg1	proteins					393:400	myofibrillar proteins	380:400	myofibrillar proteins	380:400	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	5	8	used	used	1044:1047	arg2	results					1020:1026	the results	1016:1026	the results reported	1016:1035	This study provides new insight into the factors affecting ACs stability in the EFGs, and the results reported can be used by industry to design relevant systems with extended volatiles retention.					
35247663	3	9	theme	kr	622:623	arg1	magnitudes					625:634	Higher kr magnitudes	615:634	Higher kr magnitudes	615:634	Higher kr magnitudes were found in WRS-based samples (88.2-12.8 × 10-3 day-1) than in counterparts prepared with TS (87.6-8.96 × 10-3 day-1).					
35247663	4	10	theme	Thermodynamic	757:769	arg1	tests					771:775	Thermodynamic tests	757:775	Thermodynamic tests	757:775	Thermodynamic tests revealed that vaporization enthalpies of ACs were in ranges: 22.6-41.2 kJ mol-1 or 21.4-39.8 kJ mol-1 in samples containing WRS or TS, respectively.					
35247663	1	11	theme	waxy	406:409	arg1	TS					448:449	TS	448:449	TS	448:449	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	11	theme	waxy	406:409	arg1	WRS					424:426	WRS	424:426	WRS	424:426	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	11	theme	waxy	406:409	arg1	starch					416:421	waxy rice starch	406:421	waxy rice starch (WRS)	406:427	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	0	12	dep	retention	89:97	arg1	the					85:87	the	85:87	the	85:87	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	5	13	theme	relevant	1071:1078	arg1	systems					1080:1086	relevant systems	1071:1086	relevant systems	1071:1086	This study provides new insight into the factors affecting ACs stability in the EFGs, and the results reported can be used by industry to design relevant systems with extended volatiles retention.					
35247663	5	14	theme	extended	1093:1100	arg1	retention					1112:1120	extended volatiles retention	1093:1120	extended volatiles retention	1093:1120	This study provides new insight into the factors affecting ACs stability in the EFGs, and the results reported can be used by industry to design relevant systems with extended volatiles retention.					
35247663	1	15	theme	rice	411:414	arg1	TS					448:449	TS	448:449	TS	448:449	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	15	theme	rice	411:414	arg1	WRS					424:426	WRS	424:426	WRS	424:426	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	15	theme	rice	411:414	arg1	starch					416:421	waxy rice starch	406:421	waxy rice starch (WRS)	406:427	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	2	16	with	interactions	507:518	arg1	biopolymers					532:542	biopolymers	532:542	biopolymers	532:542	The R and kr parameters were affected by non-covalent interactions of ACs with biopolymers and the development of a compact structure induced by starch addition.					
35247663	2	16	with	interactions	507:518	arg1	development					552:562	the development	548:562	the development of a compact structure induced by starch addition	548:612	The R and kr parameters were affected by non-covalent interactions of ACs with biopolymers and the development of a compact structure induced by starch addition.					
35247663	1	17	theme	aroma	245:249	arg1	compounds					251:259	aroma compounds	245:259	aroma compounds	245:259	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	2	18	theme	ACs	523:525	arg1	interactions					507:518	non-covalent interactions	494:518	non-covalent interactions of ACs with biopolymers and the development of a compact structure induced by starch addition	494:612	The R and kr parameters were affected by non-covalent interactions of ACs with biopolymers and the development of a compact structure induced by starch addition.					
35247663	2	19	theme	structure	577:585	arg1	biopolymers					532:542	biopolymers	532:542	biopolymers	532:542	The R and kr parameters were affected by non-covalent interactions of ACs with biopolymers and the development of a compact structure induced by starch addition.					
35247663	2	19	theme	structure	577:585	arg1	development					552:562	the development	548:562	the development of a compact structure induced by starch addition	548:612	The R and kr parameters were affected by non-covalent interactions of ACs with biopolymers and the development of a compact structure induced by starch addition.					
35247663	1	20	theme	compounds	251:259	arg1	kr					238:239	kr	238:239	kr	238:239	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	20	theme	compounds	251:259	arg1	ACs					262:264	ACs	262:264	ACs	262:264	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	20	theme	compounds	251:259	arg1	kinetics					228:235	release kinetics	220:235	release kinetics (kr) of aroma compounds (ACs)	220:265	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	20	theme	compounds	251:259	arg1	R					213:213	retention (R) and release kinetics (kr) of aroma compounds (ACs)	202:265	R	213:213	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	0	21	theme	compounds	120:128	arg1	release					103:109	release	103:109	release	103:109	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	0	21	theme	compounds	120:128	arg1	retention					89:97	retention	89:97	retention	89:97	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	2	22	theme	compact	569:575	arg1	structure					577:585	a compact structure	567:585	a compact structure induced by starch addition	567:612	The R and kr parameters were affected by non-covalent interactions of ACs with biopolymers and the development of a compact structure induced by starch addition.					
35247663	0	23	theme	composition	11:21	arg1	Effects					0:6	Effects	0:6	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.	0:164	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	5	24	theme	volatiles	1102:1110	arg1	retention					1112:1120	extended volatiles retention	1093:1120	extended volatiles retention	1093:1120	This study provides new insight into the factors affecting ACs stability in the EFGs, and the results reported can be used by industry to design relevant systems with extended volatiles retention.					
35247663	0	25	theme	aroma	114:118	arg1	compounds					120:128	aroma compounds	114:128	aroma compounds	114:128	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	4	26	contain	containing	890:899	arg1	samples					882:888	samples	882:888	samples containing WRS or TS	882:909	Thermodynamic tests revealed that vaporization enthalpies of ACs were in ranges: 22.6-41.2 kJ mol-1 or 21.4-39.8 kJ mol-1 in samples containing WRS or TS, respectively.					
35247663	4	26	contain	containing	890:899	arg2	TS					908:909	TS	908:909	TS	908:909	Thermodynamic tests revealed that vaporization enthalpies of ACs were in ranges: 22.6-41.2 kJ mol-1 or 21.4-39.8 kJ mol-1 in samples containing WRS or TS, respectively.					
35247663	4	26	contain	containing	890:899	arg2	WRS					901:903	WRS	901:903	WRS	901:903	Thermodynamic tests revealed that vaporization enthalpies of ACs were in ranges: 22.6-41.2 kJ mol-1 or 21.4-39.8 kJ mol-1 in samples containing WRS or TS, respectively.					
35247663	1	27	theme	retention	202:210	arg1	R					213:213	retention (R) and release kinetics (kr) of aroma compounds (ACs)	202:265	R	213:213	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	0	28	theme	time	35:38	arg1	Effects					0:6	Effects	0:6	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.	0:164	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	3	29	theme	WRS-based	650:658	arg1	samples					660:666	WRS-based samples	650:666	WRS-based samples (88.2-12.8 × 10-3 day-1)	650:691	Higher kr magnitudes were found in WRS-based samples (88.2-12.8 × 10-3 day-1) than in counterparts prepared with TS (87.6-8.96 × 10-3 day-1).					
35247663	3	29	theme	WRS-based	650:658	arg1	88.2-12.8 × 10-3 day-1					669:690	88.2-12.8 × 10-3 day-1	669:690	88.2-12.8 × 10-3 day-1	669:690	Higher kr magnitudes were found in WRS-based samples (88.2-12.8 × 10-3 day-1) than in counterparts prepared with TS (87.6-8.96 × 10-3 day-1).					
35247663	0	30	theme	Thermodynamic	131:143	arg1	studies					157:163	Thermodynamic and kinetic studies	131:163	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.	0:164	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	1	31	theme	emulsion-filled	337:351	arg1	gels					353:356	emulsion-filled gels	337:356	emulsion-filled gels (EFGs)	337:363	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	31	theme	emulsion-filled	337:351	arg1	EFGs					359:362	EFGs	359:362	EFGs	359:362	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	0	32	theme	storage	27:33	arg1	time					35:38	storage time	27:38	storage time	27:38	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	0	33	theme	biopolymers-based	43:59	arg1	gels					77:80	biopolymers-based emulsion-filled gels	43:80	biopolymers-based emulsion-filled gels	43:80	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	0	34	theme	kinetic	149:155	arg1	studies					157:163	Thermodynamic and kinetic studies	131:163	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.	0:164	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	4	35	dep	ranges	830:835	arg1	ranges					830:835	ranges	830:835	ranges: 22.6-41.2 kJ mol-1 or 21.4-39.8 kJ mol-1	830:877	Thermodynamic tests revealed that vaporization enthalpies of ACs were in ranges: 22.6-41.2 kJ mol-1 or 21.4-39.8 kJ mol-1 in samples containing WRS or TS, respectively.					
35247663	4	35	dep	ranges	830:835	arg1	21.4-39.8 kJ mol-1					860:877	21.4-39.8 kJ mol-1	860:877	21.4-39.8 kJ mol-1	860:877	Thermodynamic tests revealed that vaporization enthalpies of ACs were in ranges: 22.6-41.2 kJ mol-1 or 21.4-39.8 kJ mol-1 in samples containing WRS or TS, respectively.					
35247663	4	35	dep	ranges	830:835	arg1	22.6-41.2 kJ mol-1					838:855	22.6-41.2 kJ mol-1	838:855	22.6-41.2 kJ mol-1	838:855	Thermodynamic tests revealed that vaporization enthalpies of ACs were in ranges: 22.6-41.2 kJ mol-1 or 21.4-39.8 kJ mol-1 in samples containing WRS or TS, respectively.					
35247663	1	36	theme	ethyl	268:272	arg1	2-heptanone					308:318	2-heptanone	308:318	2-heptanone	308:318	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	36	theme	ethyl	268:272	arg1	octanal					324:330	octanal	324:330	octanal	324:330	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	36	theme	ethyl	268:272	arg1	1-octen-3-ol					294:305	1-octen-3-ol	294:305	1-octen-3-ol	294:305	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	36	theme	ethyl	268:272	arg1	linalool					284:291	linalool	284:291	linalool	284:291	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	36	theme	ethyl	268:272	arg1	butyrate					274:281	ethyl butyrate	268:281	ethyl butyrate	268:281	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	3	37	located	found	641:645	arg1	samples					660:666	WRS-based samples	650:666	WRS-based samples (88.2-12.8 × 10-3 day-1)	650:691	Higher kr magnitudes were found in WRS-based samples (88.2-12.8 × 10-3 day-1) than in counterparts prepared with TS (87.6-8.96 × 10-3 day-1).					
35247663	3	37	located	found	641:645	arg2	magnitudes					625:634	Higher kr magnitudes	615:634	Higher kr magnitudes	615:634	Higher kr magnitudes were found in WRS-based samples (88.2-12.8 × 10-3 day-1) than in counterparts prepared with TS (87.6-8.96 × 10-3 day-1).					
35247663	3	37	located	found	641:645	arg1	counterparts					701:712	counterparts	701:712	counterparts prepared with TS (87.6-8.96 × 10-3 day-1)	701:754	Higher kr magnitudes were found in WRS-based samples (88.2-12.8 × 10-3 day-1) than in counterparts prepared with TS (87.6-8.96 × 10-3 day-1).					
35247663	3	37	located	found	641:645	arg1	88.2-12.8 × 10-3 day-1					669:690	88.2-12.8 × 10-3 day-1	669:690	88.2-12.8 × 10-3 day-1	669:690	Higher kr magnitudes were found in WRS-based samples (88.2-12.8 × 10-3 day-1) than in counterparts prepared with TS (87.6-8.96 × 10-3 day-1).					
35247663	5	38	theme	ACs	985:987	arg1	stability					989:997	ACs stability	985:997	ACs stability	985:997	This study provides new insight into the factors affecting ACs stability in the EFGs, and the results reported can be used by industry to design relevant systems with extended volatiles retention.					
35247663	4	39	theme	ACs	818:820	arg1	enthalpies					804:813	vaporization enthalpies	791:813	vaporization enthalpies of ACs	791:820	Thermodynamic tests revealed that vaporization enthalpies of ACs were in ranges: 22.6-41.2 kJ mol-1 or 21.4-39.8 kJ mol-1 in samples containing WRS or TS, respectively.					
35247663	5	40	theme	new	946:948	arg1	insight					950:956	new insight	946:956	new insight into the factors affecting ACs stability in the EFGs	946:1009	This study provides new insight into the factors affecting ACs stability in the EFGs, and the results reported can be used by industry to design relevant systems with extended volatiles retention.					
35247663	4	41	from	ranges	830:835	arg1	samples					882:888	samples	882:888	samples containing WRS or TS	882:909	Thermodynamic tests revealed that vaporization enthalpies of ACs were in ranges: 22.6-41.2 kJ mol-1 or 21.4-39.8 kJ mol-1 in samples containing WRS or TS, respectively.					
35247663	1	42	theme	tapioca	432:438	arg1	starch					440:445	tapioca starch	432:445	tapioca starch	432:445	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	2	43	theme	starch	598:603	arg1	addition					605:612	starch addition	598:612	starch addition	598:612	The R and kr parameters were affected by non-covalent interactions of ACs with biopolymers and the development of a compact structure induced by starch addition.					
35247663	0	44	theme	gels	77:80	arg1	composition					11:21	composition	11:21	composition	11:21	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	0	44	theme	gels	77:80	arg1	time					35:38	storage time	27:38	storage time	27:38	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	1	45	theme	release	220:226	arg1	kr					238:239	kr	238:239	kr	238:239	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	45	theme	release	220:226	arg1	ACs					262:264	ACs	262:264	ACs	262:264	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	1	45	theme	release	220:226	arg1	kinetics					228:235	release kinetics	220:235	release kinetics (kr) of aroma compounds (ACs)	220:265	Studies were performed to determine retention (R) and release kinetics (kr) of aroma compounds (ACs): ethyl butyrate, linalool, 1-octen-3-ol, 2-heptanone and octanal from emulsion-filled gels (EFGs), stabilized by myofibrillar proteins and waxy rice starch (WRS) or tapioca starch (TS).					
35247663	0	46	theme	emulsion-filled	61:75	arg1	gels					77:80	biopolymers-based emulsion-filled gels	43:80	biopolymers-based emulsion-filled gels	43:80	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
35247663	0	47	dep	Effects	0:6	arg1	studies					157:163	Thermodynamic and kinetic studies	131:163	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.	0:164	Effects of composition and storage time of biopolymers-based emulsion-filled gels on the retention and release of aroma compounds: Thermodynamic and kinetic studies.					
37149268	0	0	theme	magnetic	89:96	arg1	alginate/biochar					98:113	magnetic alginate/biochar	89:113	magnetic alginate/biochar	89:113	Single/co-adsorption and mechanism of methylene blue and lead by β-cyclodextrin modified magnetic alginate/biochar.					
37149268	9	1	theme	Pb	1149:1150	arg1	adsorption					1160:1169	Pb (II)/MB adsorption	1149:1169	Pb (II)/MB adsorption	1149:1169	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	1	2	theme	concurrent	157:166	arg1	elimination					168:178	the concurrent elimination	153:178	the concurrent elimination of lead (Pb (II)) and methylene blue (MB)	153:220	Due to the high biological toxicity, the concurrent elimination of lead (Pb (II)) and methylene blue (MB) has become a challenging problem.					
37149268	6	3	theme	charged	801:807	arg1	MB					809:810	positively charged MB	790:810	positively charged MB	790:810	In the presence of Pb (II), MB uptake was inhibited due to the electrostatic repulsion between positively charged MB and Pb (II).					
37149268	2	4	theme	modified	297:304	arg1	MBCP					338:341	β-CD@MBCP	333:341	β-CD@MBCP	333:341	Therefore, a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material was developed.					
37149268	2	4	theme	modified	297:304	arg1	alginate/biochar					315:330	a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar	267:330	a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material	267:351	Therefore, a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material was developed.					
37149268	5	5	theme	due	655:657	arg1	MB					651:652	MB	651:652	MB	651:652	In the dual system, Pb (II) elimination was facilitated with the presence of MB, due to the active sites provided by MB.					
37149268	4	6	theme	pH	564:565	arg1	scope					567:571	a wide pH scope	557:571	a wide pH scope	557:571	The β-CD@MBCP achieved high-efficiency uptake for contaminants under a wide pH scope.					
37149268	8	7	theme	β-CD	1011:1014	arg1	MBCP					1016:1019	β-CD@MBCP	1011:1019	β-CD@MBCP	1011:1019	After four cycles, β-CD@MBCP maintained comparatively good renewability.					
37149268	2	8	dep	modified	297:304	arg1	β-CD					291:294	β-CD	291:294	β-CD	291:294	Therefore, a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material was developed.					
37149268	2	8	dep	modified	297:304	arg1	β-cyclodextrin					275:288	β-cyclodextrin	275:288	β-cyclodextrin (β-CD)	275:295	Therefore, a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material was developed.					
37149268	3	9	theme	microwave-aided	459:473	arg1	fabrication					475:485	microwave-aided fabrication	459:485	microwave-aided fabrication	459:485	Comprehensive characterizations proved the successful coating of β-CD onto MBCP surface by microwave-aided fabrication.					
37149268	4	10	theme	wide	559:562	arg1	scope					567:571	a wide pH scope	557:571	a wide pH scope	557:571	The β-CD@MBCP achieved high-efficiency uptake for contaminants under a wide pH scope.					
37149268	9	11	theme	/MB	1156:1158	arg1	adsorption					1160:1169	Pb (II)/MB adsorption	1149:1169	Pb (II)/MB adsorption	1149:1169	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	5	12	theme	dual	581:584	arg1	system					586:591	the dual system	577:591	the dual system	577:591	In the dual system, Pb (II) elimination was facilitated with the presence of MB, due to the active sites provided by MB.					
37149268	7	13	theme	Electrostatic	825:837	arg1	attraction					839:848	Electrostatic attraction	825:848	Electrostatic attraction	825:848	Electrostatic attraction and complexation contributed to capturing Pb (II), while π-π interactions, host-guest effect, and H-bonding were important in MB elimination.					
37149268	2	14	theme	@	337:337	arg1	MBCP					338:341	β-CD@MBCP	333:341	β-CD@MBCP	333:341	Therefore, a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material was developed.					
37149268	2	14	theme	@	337:337	arg1	alginate/biochar					315:330	a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar	267:330	a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material	267:351	Therefore, a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material was developed.					
37149268	5	15	theme	active	666:671	arg1	sites					673:677	the active sites	662:677	the active sites provided by MB	662:692	In the dual system, Pb (II) elimination was facilitated with the presence of MB, due to the active sites provided by MB.					
37149268	6	16	theme	electrostatic	758:770	arg1	repulsion					772:780	the electrostatic repulsion	754:780	the electrostatic repulsion between positively charged MB and Pb (II)	754:822	In the presence of Pb (II), MB uptake was inhibited due to the electrostatic repulsion between positively charged MB and Pb (II).					
37149268	1	17	theme	lead	183:186	arg1	elimination					168:178	the concurrent elimination	153:178	the concurrent elimination of lead (Pb (II)) and methylene blue (MB)	153:220	Due to the high biological toxicity, the concurrent elimination of lead (Pb (II)) and methylene blue (MB) has become a challenging problem.					
37149268	8	18	theme	good	1046:1049	arg1	renewability					1051:1062	comparatively good renewability	1032:1062	comparatively good renewability	1032:1062	After four cycles, β-CD@MBCP maintained comparatively good renewability.					
37149268	3	19	theme	Comprehensive	368:380	arg1	characterizations					382:398	Comprehensive characterizations	368:398	Comprehensive characterizations	368:398	Comprehensive characterizations proved the successful coating of β-CD onto MBCP surface by microwave-aided fabrication.					
37149268	9	20	theme	aqueous	1176:1182	arg1	environments					1184:1195	aqueous environments	1176:1195	aqueous environments	1176:1195	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	5	21	theme	Pb	594:595	arg1	elimination					602:612	Pb (II) elimination	594:612	Pb (II) elimination	594:612	In the dual system, Pb (II) elimination was facilitated with the presence of MB, due to the active sites provided by MB.					
37149268	6	22	dep	Pb	714:715	arg1	the					698:700	the	698:700	the	698:700	In the presence of Pb (II), MB uptake was inhibited due to the electrostatic repulsion between positively charged MB and Pb (II).					
37149268	6	22	dep	Pb	714:715	arg1	presence					702:709	presence	702:709	presence	702:709	In the presence of Pb (II), MB uptake was inhibited due to the electrostatic repulsion between positively charged MB and Pb (II).					
37149268	2	23	theme	β-CD	333:336	arg1	MBCP					338:341	β-CD@MBCP	333:341	β-CD@MBCP	333:341	Therefore, a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material was developed.					
37149268	2	23	theme	β-CD	333:336	arg1	alginate/biochar					315:330	a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar	267:330	a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material	267:351	Therefore, a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material was developed.					
37149268	1	24	theme	challenging	235:245	arg1	problem					247:253	a challenging problem	233:253	a challenging problem	233:253	Due to the high biological toxicity, the concurrent elimination of lead (Pb (II)) and methylene blue (MB) has become a challenging problem.					
37149268	9	25	theme	β-CD	1092:1095	arg1	MBCP					1097:1100	β-CD@MBCP	1092:1100	β-CD@MBCP	1092:1100	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	9	25	theme	β-CD	1092:1095	arg1	material					1136:1143	an effective remediation material	1111:1143	an effective remediation material for Pb (II)/MB adsorption from aqueous environments	1111:1195	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	9	26	theme	@	1096:1096	arg1	MBCP					1097:1100	β-CD@MBCP	1092:1100	β-CD@MBCP	1092:1100	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	9	26	theme	@	1096:1096	arg1	material					1136:1143	an effective remediation material	1111:1143	an effective remediation material for Pb (II)/MB adsorption from aqueous environments	1111:1195	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	3	27	theme	β-CD	433:436	arg1	coating					422:428	the successful coating	407:428	the successful coating of β-CD onto MBCP surface	407:454	Comprehensive characterizations proved the successful coating of β-CD onto MBCP surface by microwave-aided fabrication.					
37149268	0	28	theme	blue	48:51	arg1	methylene					38:46	methylene blue	38:51	methylene blue	38:51	Single/co-adsorption and mechanism of methylene blue and lead by β-cyclodextrin modified magnetic alginate/biochar.					
37149268	7	29	from	important	963:971	arg1	elimination					979:989	MB elimination	976:989	MB elimination	976:989	Electrostatic attraction and complexation contributed to capturing Pb (II), while π-π interactions, host-guest effect, and H-bonding were important in MB elimination.					
37149268	5	30	theme	MB	651:652	arg1	presence					639:646	the presence	635:646	the presence of MB, due to the active sites provided by MB	635:692	In the dual system, Pb (II) elimination was facilitated with the presence of MB, due to the active sites provided by MB.					
37149268	3	31	theme	successful	411:420	arg1	coating					422:428	the successful coating	407:428	the successful coating of β-CD onto MBCP surface	407:454	Comprehensive characterizations proved the successful coating of β-CD onto MBCP surface by microwave-aided fabrication.					
37149268	0	32	theme	methylene	38:46	arg1	mechanism					25:33	mechanism	25:33	mechanism	25:33	Single/co-adsorption and mechanism of methylene blue and lead by β-cyclodextrin modified magnetic alginate/biochar.					
37149268	0	32	theme	methylene	38:46	arg1	Single/co-adsorption					0:19	Single/co-adsorption	0:19	Single/co-adsorption	0:19	Single/co-adsorption and mechanism of methylene blue and lead by β-cyclodextrin modified magnetic alginate/biochar.					
37149268	4	33	theme	@	496:496	arg1	MBCP					497:500	The β-CD@MBCP	488:500	The β-CD@MBCP	488:500	The β-CD@MBCP achieved high-efficiency uptake for contaminants under a wide pH scope.					
37149268	7	34	theme	host-guest	925:934	arg1	effect					936:941	host-guest effect	925:941	host-guest effect	925:941	Electrostatic attraction and complexation contributed to capturing Pb (II), while π-π interactions, host-guest effect, and H-bonding were important in MB elimination.					
37149268	9	35	from	environments	1184:1195	arg1	MBCP					1097:1100	β-CD@MBCP	1092:1100	β-CD@MBCP	1092:1100	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	9	35	from	environments	1184:1195	arg1	material					1136:1143	an effective remediation material	1111:1143	an effective remediation material for Pb (II)/MB adsorption from aqueous environments	1111:1195	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	0	36	theme	lead	57:60	arg1	mechanism					25:33	mechanism	25:33	mechanism	25:33	Single/co-adsorption and mechanism of methylene blue and lead by β-cyclodextrin modified magnetic alginate/biochar.					
37149268	0	36	theme	lead	57:60	arg1	Single/co-adsorption					0:19	Single/co-adsorption	0:19	Single/co-adsorption	0:19	Single/co-adsorption and mechanism of methylene blue and lead by β-cyclodextrin modified magnetic alginate/biochar.					
37149268	4	37	theme	β-CD	492:495	arg1	MBCP					497:500	The β-CD@MBCP	488:500	The β-CD@MBCP	488:500	The β-CD@MBCP achieved high-efficiency uptake for contaminants under a wide pH scope.					
37149268	7	38	theme	π-π	907:909	arg1	interactions					911:922	π-π interactions	907:922	π-π interactions	907:922	Electrostatic attraction and complexation contributed to capturing Pb (II), while π-π interactions, host-guest effect, and H-bonding were important in MB elimination.					
37149268	4	39	theme	high-efficiency	511:525	arg1	uptake					527:532	high-efficiency uptake	511:532	high-efficiency uptake for contaminants	511:549	The β-CD@MBCP achieved high-efficiency uptake for contaminants under a wide pH scope.					
37149268	7	40	from	elimination	979:989	arg1	important					963:971	important	963:971	important	963:971	Electrostatic attraction and complexation contributed to capturing Pb (II), while π-π interactions, host-guest effect, and H-bonding were important in MB elimination.					
37149268	9	41	theme	effective	1114:1122	arg1	MBCP					1097:1100	β-CD@MBCP	1092:1100	β-CD@MBCP	1092:1100	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	9	41	theme	effective	1114:1122	arg1	material					1136:1143	an effective remediation material	1111:1143	an effective remediation material for Pb (II)/MB adsorption from aqueous environments	1111:1195	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	1	42	theme	high	127:130	arg1	toxicity					143:150	the high biological toxicity	123:150	the high biological toxicity	123:150	Due to the high biological toxicity, the concurrent elimination of lead (Pb (II)) and methylene blue (MB) has become a challenging problem.					
37149268	6	43	theme	MB	723:724	arg1	uptake					726:731	MB uptake	723:731	MB uptake	723:731	In the presence of Pb (II), MB uptake was inhibited due to the electrostatic repulsion between positively charged MB and Pb (II).					
37149268	1	44	theme	biological	132:141	arg1	toxicity					143:150	the high biological toxicity	123:150	the high biological toxicity	123:150	Due to the high biological toxicity, the concurrent elimination of lead (Pb (II)) and methylene blue (MB) has become a challenging problem.					
37149268	8	45	theme	@	1015:1015	arg1	MBCP					1016:1019	β-CD@MBCP	1011:1019	β-CD@MBCP	1011:1019	After four cycles, β-CD@MBCP maintained comparatively good renewability.					
37149268	9	46	theme	remediation	1124:1134	arg1	MBCP					1097:1100	β-CD@MBCP	1092:1100	β-CD@MBCP	1092:1100	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	9	46	theme	remediation	1124:1134	arg1	material					1136:1143	an effective remediation material	1111:1143	an effective remediation material for Pb (II)/MB adsorption from aqueous environments	1111:1195	Findings demonstrated that β-CD@MBCP could be an effective remediation material for Pb (II)/MB adsorption from aqueous environments.					
37149268	3	47	theme	MBCP	443:446	arg1	surface					448:454	MBCP surface	443:454	MBCP surface	443:454	Comprehensive characterizations proved the successful coating of β-CD onto MBCP surface by microwave-aided fabrication.					
37149268	1	48	theme	methylene	202:210	arg1	elimination					168:178	the concurrent elimination	153:178	the concurrent elimination of lead (Pb (II)) and methylene blue (MB)	153:220	Due to the high biological toxicity, the concurrent elimination of lead (Pb (II)) and methylene blue (MB) has become a challenging problem.					
37149268	7	49	theme	capturing	882:890	arg1	Pb					892:893	capturing Pb (II)	882:898	capturing Pb (II)	882:898	Electrostatic attraction and complexation contributed to capturing Pb (II), while π-π interactions, host-guest effect, and H-bonding were important in MB elimination.					
37149268	2	50	theme	magnetic	306:313	arg1	MBCP					338:341	β-CD@MBCP	333:341	β-CD@MBCP	333:341	Therefore, a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material was developed.					
37149268	2	50	theme	magnetic	306:313	arg1	alginate/biochar					315:330	a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar	267:330	a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material	267:351	Therefore, a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material was developed.					
37149268	1	51	theme	blue	212:215	arg1	methylene					202:210	methylene blue	202:215	methylene blue (MB)	202:220	Due to the high biological toxicity, the concurrent elimination of lead (Pb (II)) and methylene blue (MB) has become a challenging problem.					
37149268	1	51	theme	blue	212:215	arg1	MB					218:219	MB	218:219	MB	218:219	Due to the high biological toxicity, the concurrent elimination of lead (Pb (II)) and methylene blue (MB) has become a challenging problem.					
37149268	2	52	theme	alginate/biochar	315:330	arg1	material					344:351	a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material	267:351	a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material	267:351	Therefore, a newly β-cyclodextrin (β-CD) modified magnetic alginate/biochar (β-CD@MBCP) material was developed.					
37149268	7	53	theme	MB	976:977	arg1	elimination					979:989	MB elimination	976:989	MB elimination	976:989	Electrostatic attraction and complexation contributed to capturing Pb (II), while π-π interactions, host-guest effect, and H-bonding were important in MB elimination.					
35074101	5	0	theme	aqueous	832:838	arg1	solution					840:847	aqueous solution	832:847	aqueous solution	832:847	Molecular dynamics simulation shows that the aggregation of cellulose units in aqueous solution can enhance the adsorption of calcium ions.					
35074101	0	1	from	properties	20:29	arg1	BC/HAp					55:60	BC/HAp	55:60	BC/HAp	55:60	Enhanced mechanical properties and biocompatibility on BC/HAp composite through calcium gluconate fortified bacterial.					
35074101	6	2	theme	mechanical	938:947	arg1	properties					949:958	mechanical properties	938:958	mechanical properties	938:958	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	1	3	theme	outstanding	179:189	arg1	composite					163:171	Bacterial cellulose/hydroxyapatite (BC/HAp) composite	119:171	Bacterial cellulose/hydroxyapatite (BC/HAp) composite	119:171	Bacterial cellulose/hydroxyapatite (BC/HAp) composite is an outstanding candidate for bone tissue engineering.					
35074101	1	3	theme	outstanding	179:189	arg1	candidate					191:199	an outstanding candidate	176:199	an outstanding candidate for bone tissue engineering	176:227	Bacterial cellulose/hydroxyapatite (BC/HAp) composite is an outstanding candidate for bone tissue engineering.					
35074101	6	4	theme	conventional	1065:1076	arg1	method					1078:1083	conventional method	1065:1083	conventional method	1065:1083	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	6	5	theme	BC/HAp	988:993	arg1	composite					995:1003	the BC/HAp composite	984:1003	the BC/HAp composite	984:1003	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	6	6	theme	bone	1178:1181	arg1	engineering					1190:1200	bone tissue engineering	1178:1200	bone tissue engineering	1178:1200	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	4	7	theme	100-200 nm	619:628	arg1	size					611:614	the size	607:614	the size of 100-200 nm	607:628	Results show spherical porous HAp in the size of 100-200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization.					
35074101	3	8	theme	carbon	405:410	arg1	source					412:417	the carbon source	401:417	the carbon source to calcium gluconate	401:438	Herein, we modified the BC by changing the carbon source to calcium gluconate during the biosynthesis process of BC by bacteria, providing nucleation sites for further mineralization in simulated body fluid.					
35074101	0	9	theme	bacterial	108:116	arg1	gluconate					88:96	calcium gluconate	80:96	calcium gluconate fortified bacterial	80:116	Enhanced mechanical properties and biocompatibility on BC/HAp composite through calcium gluconate fortified bacterial.					
35074101	6	10	dep	improved	925:932	arg1	suggests					1104:1111	suggests	1104:1111	suggests	1104:1111	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	3	11	theme	body	558:561	arg1	fluid					563:567	simulated body fluid	548:567	simulated body fluid	548:567	Herein, we modified the BC by changing the carbon source to calcium gluconate during the biosynthesis process of BC by bacteria, providing nucleation sites for further mineralization in simulated body fluid.					
35074101	3	12	theme	BC	475:476	arg1	process					464:470	the biosynthesis process	447:470	the biosynthesis process of BC	447:476	Herein, we modified the BC by changing the carbon source to calcium gluconate during the biosynthesis process of BC by bacteria, providing nucleation sites for further mineralization in simulated body fluid.					
35074101	5	13	theme	cellulose	813:821	arg1	units					823:827	cellulose units	813:827	cellulose units	813:827	Molecular dynamics simulation shows that the aggregation of cellulose units in aqueous solution can enhance the adsorption of calcium ions.					
35074101	6	14	theme	preparation	1032:1042	arg1	process					1044:1050	the preparation process	1028:1050	simplified the preparation process	1017:1050	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	2	15	theme	mechanical	318:327	arg1	properties					329:338	unsatisfactory mechanical properties	303:338	unsatisfactory mechanical properties	303:338	The conventional biomimetic mineralization method takes a long time with unsatisfactory mechanical properties and biocompatibility.					
35074101	1	16	theme	bone	205:208	arg1	engineering					217:227	bone tissue engineering	205:227	bone tissue engineering	205:227	Bacterial cellulose/hydroxyapatite (BC/HAp) composite is an outstanding candidate for bone tissue engineering.					
35074101	0	17	theme	mechanical	9:18	arg1	properties					20:29	Enhanced mechanical properties	0:29	Enhanced mechanical properties	0:29	Enhanced mechanical properties and biocompatibility on BC/HAp composite through calcium gluconate fortified bacterial.					
35074101	0	18	from	biocompatibility	35:50	arg1	BC/HAp					55:60	BC/HAp	55:60	BC/HAp	55:60	Enhanced mechanical properties and biocompatibility on BC/HAp composite through calcium gluconate fortified bacterial.					
35074101	3	19	from	mineralization	530:543	arg1	fluid					563:567	simulated body fluid	548:567	simulated body fluid	548:567	Herein, we modified the BC by changing the carbon source to calcium gluconate during the biosynthesis process of BC by bacteria, providing nucleation sites for further mineralization in simulated body fluid.					
35074101	1	20	theme	tissue	210:215	arg1	engineering					217:227	bone tissue engineering	205:227	bone tissue engineering	205:227	Bacterial cellulose/hydroxyapatite (BC/HAp) composite is an outstanding candidate for bone tissue engineering.					
35074101	0	21	theme	Enhanced	0:7	arg1	properties					20:29	Enhanced mechanical properties	0:29	Enhanced mechanical properties	0:29	Enhanced mechanical properties and biocompatibility on BC/HAp composite through calcium gluconate fortified bacterial.					
35074101	5	22	theme	ions	887:890	arg1	adsorption					865:874	the adsorption	861:874	the adsorption of calcium ions	861:890	Molecular dynamics simulation shows that the aggregation of cellulose units in aqueous solution can enhance the adsorption of calcium ions.					
35074101	5	23	theme	calcium	879:885	arg1	ions					887:890	calcium ions	879:890	calcium ions	879:890	Molecular dynamics simulation shows that the aggregation of cellulose units in aqueous solution can enhance the adsorption of calcium ions.					
35074101	5	24	theme	dynamics	763:770	arg1	simulation					772:781	Molecular dynamics simulation	753:781	Molecular dynamics simulation	753:781	Molecular dynamics simulation shows that the aggregation of cellulose units in aqueous solution can enhance the adsorption of calcium ions.					
35074101	1	25	theme	Bacterial	119:127	arg1	composite					163:171	Bacterial cellulose/hydroxyapatite (BC/HAp) composite	119:171	Bacterial cellulose/hydroxyapatite (BC/HAp) composite	119:171	Bacterial cellulose/hydroxyapatite (BC/HAp) composite is an outstanding candidate for bone tissue engineering.					
35074101	1	25	theme	Bacterial	119:127	arg1	candidate					191:199	an outstanding candidate	176:199	an outstanding candidate for bone tissue engineering	176:227	Bacterial cellulose/hydroxyapatite (BC/HAp) composite is an outstanding candidate for bone tissue engineering.					
35074101	3	26	theme	calcium	422:428	arg1	gluconate					430:438	calcium gluconate	422:438	calcium gluconate	422:438	Herein, we modified the BC by changing the carbon source to calcium gluconate during the biosynthesis process of BC by bacteria, providing nucleation sites for further mineralization in simulated body fluid.					
35074101	1	27	theme	cellulose/hydroxyapatite	129:152	arg1	composite					163:171	Bacterial cellulose/hydroxyapatite (BC/HAp) composite	119:171	Bacterial cellulose/hydroxyapatite (BC/HAp) composite	119:171	Bacterial cellulose/hydroxyapatite (BC/HAp) composite is an outstanding candidate for bone tissue engineering.					
35074101	1	27	theme	cellulose/hydroxyapatite	129:152	arg1	candidate					191:199	an outstanding candidate	176:199	an outstanding candidate for bone tissue engineering	176:227	Bacterial cellulose/hydroxyapatite (BC/HAp) composite is an outstanding candidate for bone tissue engineering.					
35074101	3	28	theme	simulated	548:556	arg1	fluid					563:567	simulated body fluid	548:567	simulated body fluid	548:567	Herein, we modified the BC by changing the carbon source to calcium gluconate during the biosynthesis process of BC by bacteria, providing nucleation sites for further mineralization in simulated body fluid.					
35074101	6	29	theme	simplified	1017:1026	arg1	process					1044:1050	the preparation process	1028:1050	simplified the preparation process	1017:1050	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	5	30	theme	units	823:827	arg1	aggregation					798:808	the aggregation	794:808	the aggregation of cellulose units in aqueous solution	794:847	Molecular dynamics simulation shows that the aggregation of cellulose units in aqueous solution can enhance the adsorption of calcium ions.					
35074101	2	31	with	time	293:296	arg1	biocompatibility					344:359	biocompatibility	344:359	biocompatibility	344:359	The conventional biomimetic mineralization method takes a long time with unsatisfactory mechanical properties and biocompatibility.					
35074101	2	31	with	time	293:296	arg1	properties					329:338	unsatisfactory mechanical properties	303:338	unsatisfactory mechanical properties	303:338	The conventional biomimetic mineralization method takes a long time with unsatisfactory mechanical properties and biocompatibility.					
35074101	3	32	theme	nucleation	501:510	arg1	sites					512:516	nucleation sites	501:516	nucleation sites	501:516	Herein, we modified the BC by changing the carbon source to calcium gluconate during the biosynthesis process of BC by bacteria, providing nucleation sites for further mineralization in simulated body fluid.					
35074101	4	33	theme	spherical	583:591	arg1	HAp					600:602	spherical porous HAp in the size of 100-200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization	583:750	spherical porous HAp in the size of 100-200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization	583:750	Results show spherical porous HAp in the size of 100-200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization.					
35074101	5	34	from	aggregation	798:808	arg1	solution					840:847	aqueous solution	832:847	aqueous solution	832:847	Molecular dynamics simulation shows that the aggregation of cellulose units in aqueous solution can enhance the adsorption of calcium ions.					
35074101	6	35	theme	tissue	1183:1188	arg1	engineering					1190:1200	bone tissue engineering	1178:1200	bone tissue engineering	1178:1200	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	3	36	theme	biosynthesis	451:462	arg1	process					464:470	the biosynthesis process	447:470	the biosynthesis process of BC	447:476	Herein, we modified the BC by changing the carbon source to calcium gluconate during the biosynthesis process of BC by bacteria, providing nucleation sites for further mineralization in simulated body fluid.					
35074101	2	37	theme	biomimetic	247:256	arg1	method					273:278	The conventional biomimetic mineralization method	230:278	The conventional biomimetic mineralization method	230:278	The conventional biomimetic mineralization method takes a long time with unsatisfactory mechanical properties and biocompatibility.					
35074101	2	38	theme	mineralization	258:271	arg1	method					273:278	The conventional biomimetic mineralization method	230:278	The conventional biomimetic mineralization method	230:278	The conventional biomimetic mineralization method takes a long time with unsatisfactory mechanical properties and biocompatibility.					
35074101	1	39	theme	BC/HAp	155:160	arg1	composite					163:171	Bacterial cellulose/hydroxyapatite (BC/HAp) composite	119:171	Bacterial cellulose/hydroxyapatite (BC/HAp) composite	119:171	Bacterial cellulose/hydroxyapatite (BC/HAp) composite is an outstanding candidate for bone tissue engineering.					
35074101	1	39	theme	BC/HAp	155:160	arg1	candidate					191:199	an outstanding candidate	176:199	an outstanding candidate for bone tissue engineering	176:227	Bacterial cellulose/hydroxyapatite (BC/HAp) composite is an outstanding candidate for bone tissue engineering.					
35074101	4	40	theme	porous	593:598	arg1	HAp					600:602	spherical porous HAp in the size of 100-200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization	583:750	spherical porous HAp in the size of 100-200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization	583:750	Results show spherical porous HAp in the size of 100-200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization.					
35074101	2	41	theme	conventional	234:245	arg1	method					273:278	The conventional biomimetic mineralization method	230:278	The conventional biomimetic mineralization method	230:278	The conventional biomimetic mineralization method takes a long time with unsatisfactory mechanical properties and biocompatibility.					
35074101	2	42	theme	unsatisfactory	303:316	arg1	properties					329:338	unsatisfactory mechanical properties	303:338	unsatisfactory mechanical properties	303:338	The conventional biomimetic mineralization method takes a long time with unsatisfactory mechanical properties and biocompatibility.					
35074101	3	43	theme	further	522:528	arg1	mineralization					530:543	further mineralization	522:543	further mineralization in simulated body fluid	522:567	Herein, we modified the BC by changing the carbon source to calcium gluconate during the biosynthesis process of BC by bacteria, providing nucleation sites for further mineralization in simulated body fluid.					
35074101	6	44	dep	properties	949:958	arg1	the					934:936	the	934:936	the	934:936	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	5	45	theme	Molecular	753:761	arg1	dynamics					763:770	Molecular dynamics	753:770	Molecular dynamics simulation	753:781	Molecular dynamics simulation shows that the aggregation of cellulose units in aqueous solution can enhance the adsorption of calcium ions.					
35074101	0	46	theme	calcium	80:86	arg1	gluconate					88:96	calcium gluconate	80:96	calcium gluconate fortified bacterial	80:116	Enhanced mechanical properties and biocompatibility on BC/HAp composite through calcium gluconate fortified bacterial.					
35074101	4	47	theme	BC	693:694	arg1	structure					680:688	the three-dimensional network structure	650:688	the three-dimensional network structure of BC	650:694	Results show spherical porous HAp in the size of 100-200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization.					
35074101	4	48	theme	mineralization	737:750	arg1	days					729:732	five days	724:732	five days of mineralization	724:750	Results show spherical porous HAp in the size of 100-200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization.					
35074101	4	49	theme	network	672:678	arg1	structure					680:688	the three-dimensional network structure	650:688	the three-dimensional network structure of BC	650:694	Results show spherical porous HAp in the size of 100-200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization.					
35074101	6	50	theme	composite	995:1003	arg1	biocompatibility					964:979	biocompatibility	964:979	biocompatibility	964:979	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	6	50	theme	composite	995:1003	arg1	properties					949:958	mechanical properties	938:958	mechanical properties	938:958	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	6	50	theme	composite	995:1003	arg1	process					1044:1050	the preparation process	1028:1050	simplified the preparation process	1017:1050	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	2	51	theme	long	288:291	arg1	time					293:296	a long time	286:296	a long time with unsatisfactory mechanical properties and biocompatibility	286:359	The conventional biomimetic mineralization method takes a long time with unsatisfactory mechanical properties and biocompatibility.					
35074101	6	52	theme	biomedical	1146:1155	arg1	engineering					1190:1200	bone tissue engineering	1178:1200	bone tissue engineering	1178:1200	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	6	52	theme	biomedical	1146:1155	arg1	applications					1157:1168	biomedical applications	1146:1168	biomedical applications such as bone tissue engineering	1146:1200	By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.					
35074101	4	53	theme	three-dimensional	654:670	arg1	structure					680:688	the three-dimensional network structure	650:688	the three-dimensional network structure of BC	650:694	Results show spherical porous HAp in the size of 100-200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization.					
35166992	1	0	theme	O-antigenic	195:205	arg1	glycoforms					207:216	two O-antigenic glycoforms	191:216	two O-antigenic glycoforms	191:216	The Gram-negative bacterium Pseudomonas aeruginosa simultaneously expresses two O-antigenic glycoforms.					
35166992	6	1	theme	NMR	914:916	arg1	analysis					932:939	NMR spectroscopic analysis	914:939	NMR spectroscopic analysis of the WbpY reaction product	914:968	NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor.					
35166992	6	2	theme	α1-3	1020:1023	arg1	linkage					1025:1031	α1-3 linkage	1020:1031	α1-3 linkage	1020:1031	NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor.					
35166992	8	3	theme	WbpX	1238:1241	arg1	mixture					1227:1233	the mixture	1223:1233	the mixture of WbpX and WbpY	1223:1250	Mass spectrometry indicated that the mixture of WbpX and WbpY efficiently catalyzed the synthesis of D-Rha oligomers in a non-processive mechanism.					
35166992	5	4	contain	have	836:839	arg2	properties					841:850	properties	841:850	properties of GT-B folded enzymes of the GT4 glycosyltransferase family	841:911	In this study we showed that WbpX and WbpY recombinantly expressed in E. coli differ in their donor and acceptor specificities and have properties of GT-B folded enzymes of the GT4 glycosyltransferase family.					
35166992	5	4	contain	have	836:839	arg1	WbpX					734:737	WbpX	734:737	WbpX	734:737	In this study we showed that WbpX and WbpY recombinantly expressed in E. coli differ in their donor and acceptor specificities and have properties of GT-B folded enzymes of the GT4 glycosyltransferase family.					
35166992	6	5	theme	spectroscopic	918:930	arg1	analysis					932:939	NMR spectroscopic analysis	914:939	NMR spectroscopic analysis of the WbpY reaction product	914:968	NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor.					
35166992	3	6	theme	CH2	588:590	arg1	11-O-phenyl					592:602	D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl	567:602	D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl	567:602	We have previously reported that α3-D-Rha-transferase WbpZ transfers a D-Rha residue from GDP-D-Rha to D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl.					
35166992	6	7	theme	CH2	1077:1079	arg1	acceptor					1093:1100	synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor	1036:1100	synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor	1036:1100	NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor.					
35166992	9	8	theme	vaccine	1468:1474	arg1	development					1476:1486	vaccine development	1468:1486	vaccine development	1468:1486	Since O antigens are virulence factors, these findings open the door to advancing technology for antibacterial drug discovery and vaccine development.					
35166992	8	9	theme	Mass	1190:1193	arg1	spectrometry					1195:1206	Mass spectrometry	1190:1206	Mass spectrometry	1190:1206	Mass spectrometry indicated that the mixture of WbpX and WbpY efficiently catalyzed the synthesis of D-Rha oligomers in a non-processive mechanism.					
35166992	4	10	theme	antigen	667:673	arg1	cluster					680:686	the O antigen gene cluster	661:686	the O antigen gene cluster (wbpX and wbpY)	661:702	Genes encoding two more D-Rha-transferases are found in the O antigen gene cluster (wbpX and wbpY).					
35166992	3	11	from	GDP-D-Rha	554:562	arg1	residue					541:547	a D-Rha residue	533:547	a D-Rha residue from GDP-D-Rha to D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl	533:602	We have previously reported that α3-D-Rha-transferase WbpZ transfers a D-Rha residue from GDP-D-Rha to D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl.					
35166992	2	12	from	variable	257:264	arg1	composition					269:279	composition	269:279	composition	269:279	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	6	13	theme	D-Rha	1003:1007	arg1	residue					1009:1015	one D-Rha residue	999:1015	one D-Rha residue in α1-3 linkage	999:1031	NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor.					
35166992	9	14	theme	antibacterial	1435:1447	arg1	discovery					1454:1462	antibacterial drug discovery	1435:1462	antibacterial drug discovery	1435:1462	Since O antigens are virulence factors, these findings open the door to advancing technology for antibacterial drug discovery and vaccine development.					
35166992	6	15	from	residue	1009:1015	arg1	linkage					1025:1031	α1-3 linkage	1020:1031	α1-3 linkage	1020:1031	NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor.					
35166992	3	16	theme	α3-D-Rha-transferase	497:516	arg1	WbpZ					518:521	α3-D-Rha-transferase WbpZ	497:521	α3-D-Rha-transferase WbpZ	497:521	We have previously reported that α3-D-Rha-transferase WbpZ transfers a D-Rha residue from GDP-D-Rha to D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl.					
35166992	3	17	theme	D-GlcNAcα-O-PO3-PO3-	567:586	arg1	11-O-phenyl					592:602	D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl	567:602	D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl	567:602	We have previously reported that α3-D-Rha-transferase WbpZ transfers a D-Rha residue from GDP-D-Rha to D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl.					
35166992	2	18	theme	trisaccharide	400:412	arg1	units					424:428	trisaccharide repeating units	400:428	trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n	400:461	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	7	19	theme	α1-3	1175:1178	arg1	linkages					1180:1187	α1-3 linkages	1175:1187	α1-3 linkages	1175:1187	WbpX synthesized several products that contained D-Rha in both α1-2 and α1-3 linkages.					
35166992	9	20	theme	virulence	1359:1367	arg1	antigens					1346:1353	O antigens	1344:1353	O antigens	1344:1353	Since O antigens are virulence factors, these findings open the door to advancing technology for antibacterial drug discovery and vaccine development.					
35166992	9	20	theme	virulence	1359:1367	arg1	factors					1369:1375	virulence factors	1359:1375	virulence factors	1359:1375	Since O antigens are virulence factors, these findings open the door to advancing technology for antibacterial drug discovery and vaccine development.					
35166992	8	21	theme	WbpY	1247:1250	arg1	mixture					1227:1233	the mixture	1223:1233	the mixture of WbpX and WbpY	1223:1250	Mass spectrometry indicated that the mixture of WbpX and WbpY efficiently catalyzed the synthesis of D-Rha oligomers in a non-processive mechanism.					
35166992	1	22	dep	Pseudomonas	143:153	arg1	aeruginosa					155:164	aeruginosa	155:164	aeruginosa	155:164	The Gram-negative bacterium Pseudomonas aeruginosa simultaneously expresses two O-antigenic glycoforms.					
35166992	2	23	from	composition	269:279	arg1	variable					257:264	variable	257:264	variable	257:264	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	2	23	from	composition	269:279	arg1	antigen					240:246	the O-specific antigen	225:246	the O-specific antigen (OSA)	225:252	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	5	24	theme	acceptor	809:816	arg1	specificities					818:830	their donor and acceptor specificities	793:830	specificities	818:830	In this study we showed that WbpX and WbpY recombinantly expressed in E. coli differ in their donor and acceptor specificities and have properties of GT-B folded enzymes of the GT4 glycosyltransferase family.					
35166992	7	25	theme	several	1120:1126	arg1	products					1128:1135	several products	1120:1135	several products that contained D-Rha in both α1-2 and α1-3 linkages	1120:1187	WbpX synthesized several products that contained D-Rha in both α1-2 and α1-3 linkages.					
35166992	2	26	from	homopolymer	363:373	arg1	n					461:461	trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n	400:461	trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n	400:461	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	8	27	theme	D-Rha	1291:1295	arg1	oligomers					1297:1305	D-Rha oligomers	1291:1305	D-Rha oligomers	1291:1305	Mass spectrometry indicated that the mixture of WbpX and WbpY efficiently catalyzed the synthesis of D-Rha oligomers in a non-processive mechanism.					
35166992	8	28	theme	oligomers	1297:1305	arg1	synthesis					1278:1286	the synthesis	1274:1286	the synthesis of D-Rha oligomers	1274:1305	Mass spectrometry indicated that the mixture of WbpX and WbpY efficiently catalyzed the synthesis of D-Rha oligomers in a non-processive mechanism.					
35166992	5	29	theme	GT4	882:884	arg1	family					906:911	the GT4 glycosyltransferase family	878:911	the GT4 glycosyltransferase family	878:911	In this study we showed that WbpX and WbpY recombinantly expressed in E. coli differ in their donor and acceptor specificities and have properties of GT-B folded enzymes of the GT4 glycosyltransferase family.					
35166992	0	30	theme	Pseudomonas	20:30	arg1	antigen					65:71	the Pseudomonas aeruginosa common polysaccharide antigen	16:71	the Pseudomonas aeruginosa common polysaccharide antigen	16:71	Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY.					
35166992	0	31	dep	D-Rhamnosyltransferases	76:98	arg1	WbpX					100:103	WbpX	100:103	WbpX	100:103	Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY.					
35166992	0	31	dep	D-Rhamnosyltransferases	76:98	arg1	D-Rhamnosyltransferases					76:98	D-Rhamnosyltransferases WbpX and WbpY	76:112	D-Rhamnosyltransferases WbpX and WbpY	76:112	Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY.					
35166992	0	31	dep	D-Rhamnosyltransferases	76:98	arg1	WbpY					109:112	WbpY	109:112	WbpY	109:112	Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY.					
35166992	4	32	theme	gene	675:678	arg1	cluster					680:686	the O antigen gene cluster	661:686	the O antigen gene cluster (wbpX and wbpY)	661:702	Genes encoding two more D-Rha-transferases are found in the O antigen gene cluster (wbpX and wbpY).					
35166992	2	33	theme	O-specific	229:238	arg1	OSA					249:251	OSA	249:251	OSA	249:251	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	2	33	theme	O-specific	229:238	arg1	variable					257:264	variable	257:264	variable	257:264	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	2	33	theme	O-specific	229:238	arg1	antigen					240:246	the O-specific antigen	225:246	the O-specific antigen (OSA)	225:252	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	1	34	theme	Gram-negative	119:131	arg1	bacterium					133:141	The Gram-negative bacterium	115:141	The Gram-negative bacterium Pseudomonas aeruginosa	115:164	The Gram-negative bacterium Pseudomonas aeruginosa simultaneously expresses two O-antigenic glycoforms.					
35166992	2	35	theme	[D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3	430:459	arg1	n					461:461	trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n	400:461	trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n	400:461	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	0	36	theme	common	43:48	arg1	antigen					65:71	the Pseudomonas aeruginosa common polysaccharide antigen	16:71	the Pseudomonas aeruginosa common polysaccharide antigen	16:71	Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY.					
35166992	3	37	theme	D-Rha	535:539	arg1	residue					541:547	a D-Rha residue	533:547	a D-Rha residue from GDP-D-Rha to D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl	533:602	We have previously reported that α3-D-Rha-transferase WbpZ transfers a D-Rha residue from GDP-D-Rha to D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl.					
35166992	5	38	theme	GT-B	855:858	arg1	enzymes					867:873	GT-B folded enzymes	855:873	GT-B folded enzymes	855:873	In this study we showed that WbpX and WbpY recombinantly expressed in E. coli differ in their donor and acceptor specificities and have properties of GT-B folded enzymes of the GT4 glycosyltransferase family.					
35166992	4	39	located	found	652:656	arg1	cluster					680:686	the O antigen gene cluster	661:686	the O antigen gene cluster (wbpX and wbpY)	661:702	Genes encoding two more D-Rha-transferases are found in the O antigen gene cluster (wbpX and wbpY).					
35166992	4	39	located	found	652:656	arg2	Genes					605:609	Genes	605:609	Genes encoding two more D-Rha-transferases	605:646	Genes encoding two more D-Rha-transferases are found in the O antigen gene cluster (wbpX and wbpY).					
35166992	2	40	theme	units	424:428	arg1	n					461:461	trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n	400:461	trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n	400:461	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	5	41	theme	enzymes	867:873	arg1	properties					841:850	properties	841:850	properties of GT-B folded enzymes of the GT4 glycosyltransferase family	841:911	In this study we showed that WbpX and WbpY recombinantly expressed in E. coli differ in their donor and acceptor specificities and have properties of GT-B folded enzymes of the GT4 glycosyltransferase family.					
35166992	9	42	theme	advancing	1410:1418	arg1	technology					1420:1429	advancing technology	1410:1429	advancing technology	1410:1429	Since O antigens are virulence factors, these findings open the door to advancing technology for antibacterial drug discovery and vaccine development.					
35166992	8	43	theme	non-processive	1312:1325	arg1	mechanism					1327:1335	a non-processive mechanism	1310:1335	a non-processive mechanism	1310:1335	Mass spectrometry indicated that the mixture of WbpX and WbpY efficiently catalyzed the synthesis of D-Rha oligomers in a non-processive mechanism.					
35166992	4	44	dep	cluster	680:686	arg1	wbpY					698:701	wbpY	698:701	wbpY	698:701	Genes encoding two more D-Rha-transferases are found in the O antigen gene cluster (wbpX and wbpY).					
35166992	4	44	dep	cluster	680:686	arg1	wbpX					689:692	wbpX	689:692	wbpX	689:692	Genes encoding two more D-Rha-transferases are found in the O antigen gene cluster (wbpX and wbpY).					
35166992	2	45	theme	repeating	414:422	arg1	units					424:428	trisaccharide repeating units	400:428	trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n	400:461	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	0	46	theme	antigen	65:71	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY.	0:113	Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY.					
35166992	6	47	theme	product	962:968	arg1	analysis					932:939	NMR spectroscopic analysis	914:939	NMR spectroscopic analysis of the WbpY reaction product	914:968	NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor.					
35166992	0	48	theme	polysaccharide	50:63	arg1	antigen					65:71	the Pseudomonas aeruginosa common polysaccharide antigen	16:71	the Pseudomonas aeruginosa common polysaccharide antigen	16:71	Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY.					
35166992	6	49	theme	reaction	953:960	arg1	product					962:968	the WbpY reaction product	944:968	the WbpY reaction product	944:968	NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor.					
35166992	1	50	theme	Pseudomonas	143:153	arg1	bacterium					133:141	The Gram-negative bacterium	115:141	The Gram-negative bacterium Pseudomonas aeruginosa	115:164	The Gram-negative bacterium Pseudomonas aeruginosa simultaneously expresses two O-antigenic glycoforms.					
35166992	6	51	theme	WbpY	948:951	arg1	product					962:968	the WbpY reaction product	944:968	the WbpY reaction product	944:968	NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor.					
35166992	2	52	theme	polysaccharide	293:306	arg1	CPA					317:319	CPA	317:319	CPA	317:319	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	2	52	theme	polysaccharide	293:306	arg1	antigen					308:314	the common polysaccharide antigen	282:314	the common polysaccharide antigen (CPA)	282:320	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	0	53	dep	Pseudomonas	20:30	arg1	aeruginosa					32:41	aeruginosa	32:41	aeruginosa	32:41	Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY.					
35166992	6	54	theme	D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-	1046:1075	arg1	acceptor					1093:1100	synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor	1036:1100	synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor	1036:1100	NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor.					
35166992	9	55	theme	O	1344:1344	arg1	antigens					1346:1353	O antigens	1344:1353	O antigens	1344:1353	Since O antigens are virulence factors, these findings open the door to advancing technology for antibacterial drug discovery and vaccine development.					
35166992	9	55	theme	O	1344:1344	arg1	factors					1369:1375	virulence factors	1359:1375	virulence factors	1359:1375	Since O antigens are virulence factors, these findings open the door to advancing technology for antibacterial drug discovery and vaccine development.					
35166992	2	56	theme	common	286:291	arg1	CPA					317:319	CPA	317:319	CPA	317:319	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	2	56	theme	common	286:291	arg1	antigen					308:314	the common polysaccharide antigen	282:314	the common polysaccharide antigen (CPA)	282:320	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	5	57	theme	family	906:911	arg1	properties					841:850	properties	841:850	properties of GT-B folded enzymes of the GT4 glycosyltransferase family	841:911	In this study we showed that WbpX and WbpY recombinantly expressed in E. coli differ in their donor and acceptor specificities and have properties of GT-B folded enzymes of the GT4 glycosyltransferase family.					
35166992	7	58	contain	contained	1142:1150	arg1	linkages					1180:1187	α1-3 linkages	1175:1187	α1-3 linkages	1175:1187	WbpX synthesized several products that contained D-Rha in both α1-2 and α1-3 linkages.					
35166992	7	58	contain	contained	1142:1150	arg1	α1-2					1166:1169	α1-2	1166:1169	α1-2	1166:1169	WbpX synthesized several products that contained D-Rha in both α1-2 and α1-3 linkages.					
35166992	7	58	contain	contained	1142:1150	arg1	products					1128:1135	several products	1120:1135	several products that contained D-Rha in both α1-2 and α1-3 linkages	1120:1187	WbpX synthesized several products that contained D-Rha in both α1-2 and α1-3 linkages.					
35166992	7	58	contain	contained	1142:1150	arg2	products					1128:1135	several products	1120:1135	several products that contained D-Rha in both α1-2 and α1-3 linkages	1120:1187	WbpX synthesized several products that contained D-Rha in both α1-2 and α1-3 linkages.					
35166992	7	58	contain	contained	1142:1150	arg2	D-Rha					1152:1156	D-Rha	1152:1156	D-Rha	1152:1156	WbpX synthesized several products that contained D-Rha in both α1-2 and α1-3 linkages.					
35166992	6	59	theme	synthetic	1036:1044	arg1	acceptor					1093:1100	synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor	1036:1100	synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor	1036:1100	NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor.					
35166992	5	60	theme	folded	860:865	arg1	enzymes					867:873	GT-B folded enzymes	855:873	GT-B folded enzymes	855:873	In this study we showed that WbpX and WbpY recombinantly expressed in E. coli differ in their donor and acceptor specificities and have properties of GT-B folded enzymes of the GT4 glycosyltransferase family.					
35166992	6	61	theme	11-O-phenyl	1081:1091	arg1	acceptor					1093:1100	synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor	1036:1100	synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor	1036:1100	NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor.					
35166992	5	62	theme	glycosyltransferase	886:904	arg1	family					906:911	the GT4 glycosyltransferase family	878:911	the GT4 glycosyltransferase family	878:911	In this study we showed that WbpX and WbpY recombinantly expressed in E. coli differ in their donor and acceptor specificities and have properties of GT-B folded enzymes of the GT4 glycosyltransferase family.					
35166992	4	63	theme	O	665:665	arg1	cluster					680:686	the O antigen gene cluster	661:686	the O antigen gene cluster (wbpX and wbpY)	661:702	Genes encoding two more D-Rha-transferases are found in the O antigen gene cluster (wbpX and wbpY).					
35166992	2	64	theme	D-rhamnose	378:387	arg1	homopolymer					363:373	a homopolymer	361:373	a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n	361:461	While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n.					
35166992	9	65	theme	drug	1449:1452	arg1	discovery					1454:1462	antibacterial drug discovery	1435:1462	antibacterial drug discovery	1435:1462	Since O antigens are virulence factors, these findings open the door to advancing technology for antibacterial drug discovery and vaccine development.					
35378161	6	0	theme	crosslinked	1139:1149	arg1	hydrogels					1151:1159	physically crosslinked hydrogels	1128:1159	physically crosslinked hydrogels	1128:1159	We demonstrated that physically crosslinked hydrogels inhibited over 99.999% of Gram-negative E. coli, while chemically crosslinking strategy led to the antibacterial efficiency decrease.					
35378161	2	1	theme	current	351:357	arg1	study					359:363	the current study	347:363	the current study	347:363	In the current study, we have designed hyaluronic acid and ɛ-polylysine composite hydrogel systems with antibacterial and cell attractive properties.					
35378161	4	2	theme	in	872:873	arg1	biocompatibility					881:896	in vitro biocompatibility	872:896	in vitro biocompatibility	872:896	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	4	3	from	impact	684:689	arg1	properties					832:841	viscoelastic properties	819:841	viscoelastic properties	819:841	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	4	3	from	impact	684:689	arg1	structure					753:761	fabricated hydrogel molecular structure	723:761	fabricated hydrogel molecular structure	723:761	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	4	3	from	impact	684:689	arg1	fraction					787:794	gel fraction	783:794	gel fraction	783:794	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	4	3	from	impact	684:689	arg1	biocompatibility					881:896	in vitro biocompatibility	872:896	in vitro biocompatibility	872:896	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	4	3	from	impact	684:689	arg1	morphology					797:806	morphology	797:806	morphology	797:806	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	4	3	from	impact	684:689	arg1	activity					858:865	antibacterial activity	844:865	antibacterial activity	844:865	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	4	3	from	impact	684:689	arg1	behavior					773:780	swelling behavior	764:780	swelling behavior	764:780	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	4	3	from	impact	684:689	arg1	porosity					809:816	porosity	809:816	porosity	809:816	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	1	4	theme	bioactive	199:207	arg1	acid					220:223	bioactive hyaluronic acid	199:223	bioactive hyaluronic acid (HA)	199:228	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	1	4	theme	bioactive	199:207	arg1	HA					226:227	HA	226:227	HA	226:227	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	0	5	theme	ɛ-polylysine	120:131	arg1	hydrogels					139:147	ɛ-polylysine based hydrogels	120:147	ɛ-polylysine based hydrogels	120:147	Effect of crosslinking strategy on the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels.					
35378161	3	6	dep	groups	655:660	arg1	groups					655:660	charged functional groups	636:660	charged functional groups (-NH2 and -COOH)	636:677	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	3	6	dep	groups	655:660	arg1	-COOH					672:676	-COOH	672:676	-COOH	672:676	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	3	6	dep	groups	655:660	arg1	-NH2					663:666	-NH2	663:666	-NH2	663:666	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	0	7	theme	hydrogels	139:147	arg1	performance					85:95	the biological, antibacterial and physicochemical performance	35:95	the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels	35:147	Effect of crosslinking strategy on the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels.					
35378161	0	8	theme	strategy	23:30	arg1	Effect					0:5	Effect	0:5	Effect of crosslinking strategy on the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels.	0:148	Effect of crosslinking strategy on the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels.					
35378161	7	9	theme	in	1444:1445	arg1	biocompatibility					1453:1468	in vitro biocompatibility	1444:1468	in vitro biocompatibility	1444:1468	However, cell viability was significantly improved, confirming the importance of the applied crosslinking approach to the antibacterial activity and in vitro biocompatibility.					
35378161	4	10	theme	molecular	743:751	arg1	structure					753:761	fabricated hydrogel molecular structure	723:761	fabricated hydrogel molecular structure	723:761	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	5	11	theme	stiffness	1084:1092	arg1	28 days					1047:1053	28 days	1047:1053	28 days	1047:1053	Both chemically and physically crosslinked HA/ԑ-PL hydrogels demonstrated fast swelling behavior and long-term stability for at least 28 days, as well as similar order of stiffness (10-30 kPa).					
35378161	5	11	theme	stiffness	1084:1092	arg1	order					1075:1079	similar order	1067:1079	similar order of stiffness (10-30 kPa)	1067:1104	Both chemically and physically crosslinked HA/ԑ-PL hydrogels demonstrated fast swelling behavior and long-term stability for at least 28 days, as well as similar order of stiffness (10-30 kPa).					
35378161	8	12	theme	materials	1562:1570	arg1	properties					1534:1543	the physicochemical and biological properties	1499:1543	the physicochemical and biological properties of the developed materials	1499:1570	The distinct differences in the physicochemical and biological properties of the developed materials provide new opportunities to design next-generation functional composite hydrogel systems.					
35378161	6	13	theme	antibacterial	1260:1272	arg1	decrease					1285:1292	the antibacterial efficiency decrease	1256:1292	the antibacterial efficiency decrease	1256:1292	We demonstrated that physically crosslinked hydrogels inhibited over 99.999% of Gram-negative E. coli, while chemically crosslinking strategy led to the antibacterial efficiency decrease.					
35378161	5	14	theme	fast	987:990	arg1	behavior					1001:1008	fast swelling behavior	987:1008	fast swelling behavior	987:1008	Both chemically and physically crosslinked HA/ԑ-PL hydrogels demonstrated fast swelling behavior and long-term stability for at least 28 days, as well as similar order of stiffness (10-30 kPa).					
35378161	8	15	theme	new	1580:1582	arg1	opportunities					1584:1596	new opportunities	1580:1596	new opportunities to design next-generation functional composite hydrogel systems	1580:1660	The distinct differences in the physicochemical and biological properties of the developed materials provide new opportunities to design next-generation functional composite hydrogel systems.					
35378161	4	16	theme	strategy	711:718	arg1	impact					684:689	The impact	680:689	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility	680:896	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	0	17	theme	antibacterial	51:63	arg1	performance					85:95	the biological, antibacterial and physicochemical performance	35:95	the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels	35:147	Effect of crosslinking strategy on the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels.					
35378161	3	18	theme	groups	655:660	arg1	attractions					591:601	electrostatic attractions	577:601	electrostatic attractions	577:601	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	3	18	theme	groups	655:660	arg1	crosslinking					620:631	the chemical crosslinking	607:631	the chemical crosslinking of charged functional groups (-NH2 and -COOH)	607:677	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	4	19	theme	fabricated	723:732	arg1	structure					753:761	fabricated hydrogel molecular structure	723:761	fabricated hydrogel molecular structure	723:761	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	0	20	theme	physicochemical	69:83	arg1	performance					85:95	the biological, antibacterial and physicochemical performance	35:95	the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels	35:147	Effect of crosslinking strategy on the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels.					
35378161	1	21	theme	multifunctional	164:178	arg1	hydrogels					180:188	multifunctional hydrogels	164:188	multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL)	164:286	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	8	22	theme	next-generation	1608:1622	arg1	systems					1654:1660	design next-generation functional composite hydrogel systems	1601:1660	design next-generation functional composite hydrogel systems	1601:1660	The distinct differences in the physicochemical and biological properties of the developed materials provide new opportunities to design next-generation functional composite hydrogel systems.					
35378161	8	23	theme	composite	1635:1643	arg1	systems					1654:1660	design next-generation functional composite hydrogel systems	1601:1660	design next-generation functional composite hydrogel systems	1601:1660	The distinct differences in the physicochemical and biological properties of the developed materials provide new opportunities to design next-generation functional composite hydrogel systems.					
35378161	0	24	theme	acid	111:114	arg1	performance					85:95	the biological, antibacterial and physicochemical performance	35:95	the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels	35:147	Effect of crosslinking strategy on the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels.					
35378161	7	25	theme	crosslinking	1388:1399	arg1	approach					1401:1408	the applied crosslinking approach	1376:1408	the applied crosslinking approach	1376:1408	However, cell viability was significantly improved, confirming the importance of the applied crosslinking approach to the antibacterial activity and in vitro biocompatibility.					
35378161	4	26	theme	gel	783:785	arg1	fraction					787:794	gel fraction	783:794	gel fraction	783:794	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	4	27	theme	antibacterial	844:856	arg1	activity					858:865	antibacterial activity	844:865	antibacterial activity	844:865	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	2	28	theme	hydrogel	426:433	arg1	systems					435:441	ɛ-polylysine composite hydrogel systems	403:441	ɛ-polylysine composite hydrogel systems with antibacterial and cell attractive properties	403:491	In the current study, we have designed hyaluronic acid and ɛ-polylysine composite hydrogel systems with antibacterial and cell attractive properties.					
35378161	0	29	theme	crosslinking	10:21	arg1	strategy					23:30	crosslinking strategy	10:30	crosslinking strategy	10:30	Effect of crosslinking strategy on the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels.					
35378161	5	30	link	crosslinked	944:954	arg1	hydrogels					964:972	Both chemically and physically crosslinked HA/ԑ-PL hydrogels	913:972	Both chemically and physically crosslinked HA/ԑ-PL hydrogels	913:972	Both chemically and physically crosslinked HA/ԑ-PL hydrogels demonstrated fast swelling behavior and long-term stability for at least 28 days, as well as similar order of stiffness (10-30 kPa).					
35378161	5	31	theme	long-term	1014:1022	arg1	stability					1024:1032	long-term stability	1014:1032	long-term stability	1014:1032	Both chemically and physically crosslinked HA/ԑ-PL hydrogels demonstrated fast swelling behavior and long-term stability for at least 28 days, as well as similar order of stiffness (10-30 kPa).					
35378161	8	32	theme	distinct	1475:1482	arg1	differences					1484:1494	The distinct differences	1471:1494	The distinct differences in the physicochemical and biological properties of the developed materials	1471:1570	The distinct differences in the physicochemical and biological properties of the developed materials provide new opportunities to design next-generation functional composite hydrogel systems.					
35378161	2	33	theme	ɛ-polylysine	403:414	arg1	systems					435:441	ɛ-polylysine composite hydrogel systems	403:441	ɛ-polylysine composite hydrogel systems with antibacterial and cell attractive properties	403:491	In the current study, we have designed hyaluronic acid and ɛ-polylysine composite hydrogel systems with antibacterial and cell attractive properties.					
35378161	7	34	dep	in	1444:1445	arg1	vitro					1447:1451	vitro	1447:1451	vitro	1447:1451	However, cell viability was significantly improved, confirming the importance of the applied crosslinking approach to the antibacterial activity and in vitro biocompatibility.					
35378161	5	35	theme	similar	1067:1073	arg1	order					1075:1079	similar order	1067:1079	similar order of stiffness (10-30 kPa)	1067:1104	Both chemically and physically crosslinked HA/ԑ-PL hydrogels demonstrated fast swelling behavior and long-term stability for at least 28 days, as well as similar order of stiffness (10-30 kPa).					
35378161	2	36	theme	attractive	471:480	arg1	properties					482:491	antibacterial and cell attractive properties	448:491	antibacterial and cell attractive properties	448:491	In the current study, we have designed hyaluronic acid and ɛ-polylysine composite hydrogel systems with antibacterial and cell attractive properties.					
35378161	3	37	theme	crosslinking	507:518	arg1	approaches					520:529	Two distinct crosslinking approaches	494:529	Two distinct crosslinking approaches	494:529	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	0	38	theme	biological	39:48	arg1	performance					85:95	the biological, antibacterial and physicochemical performance	35:95	the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels	35:147	Effect of crosslinking strategy on the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels.					
35378161	1	39	theme	tissue	311:316	arg1	applications					330:341	tissue engineering applications	311:341	tissue engineering applications	311:341	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	3	40	used	used	536:539	arg2	approaches					520:529	Two distinct crosslinking approaches	494:529	Two distinct crosslinking approaches	494:529	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	8	41	theme	biological	1523:1532	arg1	properties					1534:1543	the physicochemical and biological properties	1499:1543	the physicochemical and biological properties of the developed materials	1499:1570	The distinct differences in the physicochemical and biological properties of the developed materials provide new opportunities to design next-generation functional composite hydrogel systems.					
35378161	1	42	theme	antibacterial	234:246	arg1	ɛ-poly-l-lysine					265:279	antibacterial cationic polymer ɛ-poly-l-lysine	234:279	antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL)	234:286	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	1	42	theme	antibacterial	234:246	arg1	ε-PL					282:285	ε-PL	282:285	ε-PL	282:285	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	1	43	theme	polymer	257:263	arg1	ɛ-poly-l-lysine					265:279	antibacterial cationic polymer ɛ-poly-l-lysine	234:279	antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL)	234:286	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	1	43	theme	polymer	257:263	arg1	ε-PL					282:285	ε-PL	282:285	ε-PL	282:285	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	2	44	with	acid	394:397	arg1	properties					482:491	antibacterial and cell attractive properties	448:491	antibacterial and cell attractive properties	448:491	In the current study, we have designed hyaluronic acid and ɛ-polylysine composite hydrogel systems with antibacterial and cell attractive properties.					
35378161	7	45	theme	cell	1304:1307	arg1	viability					1309:1317	cell viability	1304:1317	cell viability	1304:1317	However, cell viability was significantly improved, confirming the importance of the applied crosslinking approach to the antibacterial activity and in vitro biocompatibility.					
35378161	1	46	from	tool	303:306	arg1	applications					330:341	tissue engineering applications	311:341	tissue engineering applications	311:341	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	6	47	theme	crosslinking	1227:1238	arg1	strategy					1240:1247	chemically crosslinking strategy	1216:1247	chemically crosslinking strategy	1216:1247	We demonstrated that physically crosslinked hydrogels inhibited over 99.999% of Gram-negative E. coli, while chemically crosslinking strategy led to the antibacterial efficiency decrease.					
35378161	3	48	theme	physical	546:553	arg1	crosslinking					555:566	the physical crosslinking	542:566	the physical crosslinking	542:566	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	8	49	from	differences	1484:1494	arg1	properties					1534:1543	the physicochemical and biological properties	1499:1543	the physicochemical and biological properties of the developed materials	1499:1570	The distinct differences in the physicochemical and biological properties of the developed materials provide new opportunities to design next-generation functional composite hydrogel systems.					
35378161	1	50	theme	hydrogels	180:188	arg1	design					154:159	The design	150:159	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL)	150:286	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	1	50	theme	hydrogels	180:188	arg1	tool					303:306	a promising tool	291:306	a promising tool in tissue engineering applications	291:341	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	6	51	theme	coli	1204:1207	arg1	coli					1204:1207	Gram-negative E. coli	1187:1207	Gram-negative E. coli	1187:1207	We demonstrated that physically crosslinked hydrogels inhibited over 99.999% of Gram-negative E. coli, while chemically crosslinking strategy led to the antibacterial efficiency decrease.					
35378161	6	51	theme	coli	1204:1207	arg1	%					1182:1182	99.999%	1176:1182	99.999% of Gram-negative E. coli	1176:1207	We demonstrated that physically crosslinked hydrogels inhibited over 99.999% of Gram-negative E. coli, while chemically crosslinking strategy led to the antibacterial efficiency decrease.					
35378161	0	52	theme	based	133:137	arg1	hydrogels					139:147	ɛ-polylysine based hydrogels	120:147	ɛ-polylysine based hydrogels	120:147	Effect of crosslinking strategy on the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels.					
35378161	3	53	theme	electrostatic	577:589	arg1	attractions					591:601	electrostatic attractions	577:601	electrostatic attractions	577:601	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	1	54	theme	hyaluronic	209:218	arg1	acid					220:223	bioactive hyaluronic acid	199:223	bioactive hyaluronic acid (HA)	199:228	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	1	54	theme	hyaluronic	209:218	arg1	HA					226:227	HA	226:227	HA	226:227	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	5	55	theme	crosslinked	944:954	arg1	hydrogels					964:972	Both chemically and physically crosslinked HA/ԑ-PL hydrogels	913:972	Both chemically and physically crosslinked HA/ԑ-PL hydrogels	913:972	Both chemically and physically crosslinked HA/ԑ-PL hydrogels demonstrated fast swelling behavior and long-term stability for at least 28 days, as well as similar order of stiffness (10-30 kPa).					
35378161	8	56	theme	developed	1552:1560	arg1	materials					1562:1570	the developed materials	1548:1570	the developed materials	1548:1570	The distinct differences in the physicochemical and biological properties of the developed materials provide new opportunities to design next-generation functional composite hydrogel systems.					
35378161	3	57	theme	chemical	611:618	arg1	crosslinking					620:631	the chemical crosslinking	607:631	the chemical crosslinking of charged functional groups (-NH2 and -COOH)	607:677	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	2	58	with	systems	435:441	arg1	properties					482:491	antibacterial and cell attractive properties	448:491	antibacterial and cell attractive properties	448:491	In the current study, we have designed hyaluronic acid and ɛ-polylysine composite hydrogel systems with antibacterial and cell attractive properties.					
35378161	5	59	theme	HA/ԑ-PL	956:962	arg1	hydrogels					964:972	Both chemically and physically crosslinked HA/ԑ-PL hydrogels	913:972	Both chemically and physically crosslinked HA/ԑ-PL hydrogels	913:972	Both chemically and physically crosslinked HA/ԑ-PL hydrogels demonstrated fast swelling behavior and long-term stability for at least 28 days, as well as similar order of stiffness (10-30 kPa).					
35378161	0	60	from	Effect	0:5	arg1	performance					85:95	the biological, antibacterial and physicochemical performance	35:95	the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels	35:147	Effect of crosslinking strategy on the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels.					
35378161	6	61	theme	efficiency	1274:1283	arg1	decrease					1285:1292	the antibacterial efficiency decrease	1256:1292	the antibacterial efficiency decrease	1256:1292	We demonstrated that physically crosslinked hydrogels inhibited over 99.999% of Gram-negative E. coli, while chemically crosslinking strategy led to the antibacterial efficiency decrease.					
35378161	4	62	theme	hydrogel	734:741	arg1	structure					753:761	fabricated hydrogel molecular structure	723:761	fabricated hydrogel molecular structure	723:761	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	8	63	theme	physicochemical	1503:1517	arg1	properties					1534:1543	the physicochemical and biological properties	1499:1543	the physicochemical and biological properties of the developed materials	1499:1570	The distinct differences in the physicochemical and biological properties of the developed materials provide new opportunities to design next-generation functional composite hydrogel systems.					
35378161	3	64	theme	functional	644:653	arg1	groups					655:660	charged functional groups	636:660	charged functional groups (-NH2 and -COOH)	636:677	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	3	64	theme	functional	644:653	arg1	-COOH					672:676	-COOH	672:676	-COOH	672:676	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	3	64	theme	functional	644:653	arg1	-NH2					663:666	-NH2	663:666	-NH2	663:666	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	8	65	theme	design	1601:1606	arg1	systems					1654:1660	design next-generation functional composite hydrogel systems	1601:1660	design next-generation functional composite hydrogel systems	1601:1660	The distinct differences in the physicochemical and biological properties of the developed materials provide new opportunities to design next-generation functional composite hydrogel systems.					
35378161	7	66	theme	antibacterial	1417:1429	arg1	activity					1431:1438	the antibacterial activity	1413:1438	the antibacterial activity	1413:1438	However, cell viability was significantly improved, confirming the importance of the applied crosslinking approach to the antibacterial activity and in vitro biocompatibility.					
35378161	4	67	theme	crosslinking	698:709	arg1	strategy					711:718	the crosslinking strategy	694:718	the crosslinking strategy	694:718	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	8	68	theme	functional	1624:1633	arg1	systems					1654:1660	design next-generation functional composite hydrogel systems	1601:1660	design next-generation functional composite hydrogel systems	1601:1660	The distinct differences in the physicochemical and biological properties of the developed materials provide new opportunities to design next-generation functional composite hydrogel systems.					
35378161	0	69	theme	hyaluronic	100:109	arg1	acid					111:114	hyaluronic acid	100:114	hyaluronic acid	100:114	Effect of crosslinking strategy on the biological, antibacterial and physicochemical performance of hyaluronic acid and ɛ-polylysine based hydrogels.					
35378161	8	70	theme	hydrogel	1645:1652	arg1	systems					1654:1660	design next-generation functional composite hydrogel systems	1601:1660	design next-generation functional composite hydrogel systems	1601:1660	The distinct differences in the physicochemical and biological properties of the developed materials provide new opportunities to design next-generation functional composite hydrogel systems.					
35378161	7	71	theme	approach	1401:1408	arg1	importance					1362:1371	the importance	1358:1371	the importance of the applied crosslinking approach to the antibacterial activity and in vitro biocompatibility	1358:1468	However, cell viability was significantly improved, confirming the importance of the applied crosslinking approach to the antibacterial activity and in vitro biocompatibility.					
35378161	2	72	theme	hyaluronic	383:392	arg1	acid					394:397	hyaluronic acid	383:397	hyaluronic acid	383:397	In the current study, we have designed hyaluronic acid and ɛ-polylysine composite hydrogel systems with antibacterial and cell attractive properties.					
35378161	5	73	theme	swelling	992:999	arg1	behavior					1001:1008	fast swelling behavior	987:1008	fast swelling behavior	987:1008	Both chemically and physically crosslinked HA/ԑ-PL hydrogels demonstrated fast swelling behavior and long-term stability for at least 28 days, as well as similar order of stiffness (10-30 kPa).					
35378161	7	74	theme	applied	1380:1386	arg1	approach					1401:1408	the applied crosslinking approach	1376:1408	the applied crosslinking approach	1376:1408	However, cell viability was significantly improved, confirming the importance of the applied crosslinking approach to the antibacterial activity and in vitro biocompatibility.					
35378161	2	75	theme	antibacterial	448:460	arg1	properties					482:491	antibacterial and cell attractive properties	448:491	antibacterial and cell attractive properties	448:491	In the current study, we have designed hyaluronic acid and ɛ-polylysine composite hydrogel systems with antibacterial and cell attractive properties.					
35378161	4	76	theme	swelling	764:771	arg1	behavior					773:780	swelling behavior	764:780	swelling behavior	764:780	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	4	77	dep	in	872:873	arg1	vitro					875:879	vitro	875:879	vitro	875:879	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	2	78	theme	composite	416:424	arg1	systems					435:441	ɛ-polylysine composite hydrogel systems	403:441	ɛ-polylysine composite hydrogel systems with antibacterial and cell attractive properties	403:491	In the current study, we have designed hyaluronic acid and ɛ-polylysine composite hydrogel systems with antibacterial and cell attractive properties.					
35378161	1	79	theme	promising	293:301	arg1	design					154:159	The design	150:159	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL)	150:286	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	1	79	theme	promising	293:301	arg1	tool					303:306	a promising tool	291:306	a promising tool in tissue engineering applications	291:341	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	6	80	link	crosslinked	1139:1149	arg1	hydrogels					1151:1159	physically crosslinked hydrogels	1128:1159	physically crosslinked hydrogels	1128:1159	We demonstrated that physically crosslinked hydrogels inhibited over 99.999% of Gram-negative E. coli, while chemically crosslinking strategy led to the antibacterial efficiency decrease.					
35378161	1	81	theme	engineering	318:328	arg1	applications					330:341	tissue engineering applications	311:341	tissue engineering applications	311:341	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	3	82	theme	charged	636:642	arg1	groups					655:660	charged functional groups	636:660	charged functional groups (-NH2 and -COOH)	636:677	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	3	82	theme	charged	636:642	arg1	-COOH					672:676	-COOH	672:676	-COOH	672:676	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	3	82	theme	charged	636:642	arg1	-NH2					663:666	-NH2	663:666	-NH2	663:666	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	3	83	theme	distinct	498:505	arg1	approaches					520:529	Two distinct crosslinking approaches	494:529	Two distinct crosslinking approaches	494:529	Two distinct crosslinking approaches were used: the physical crosslinking based on electrostatic attractions and the chemical crosslinking of charged functional groups (-NH2 and -COOH).					
35378161	4	84	theme	viscoelastic	819:830	arg1	properties					832:841	viscoelastic properties	819:841	viscoelastic properties	819:841	The impact of the crosslinking strategy on fabricated hydrogel molecular structure, swelling behavior, gel fraction, morphology, porosity, viscoelastic properties, antibacterial activity, and in vitro biocompatibility was evaluated.					
35378161	1	85	theme	cationic	248:255	arg1	ɛ-poly-l-lysine					265:279	antibacterial cationic polymer ɛ-poly-l-lysine	234:279	antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL)	234:286	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35378161	1	85	theme	cationic	248:255	arg1	ε-PL					282:285	ε-PL	282:285	ε-PL	282:285	The design of multifunctional hydrogels based on bioactive hyaluronic acid (HA) and antibacterial cationic polymer ɛ-poly-l-lysine (ε-PL) is a promising tool in tissue engineering applications.					
35171615	8	0	theme	fluorescence	1079:1090	arg1	micrographs					1092:1102	The fluorescence micrographs	1075:1102	The fluorescence micrographs	1075:1102	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	1	1	theme	key	141:143	arg1	strategies					145:154	the key strategies	137:154	the key strategies in interfacial engineering for emulsion stability	137:204	Emulsifier design is one of the key strategies in interfacial engineering for emulsion stability.					
35171615	2	2	used	used	257:260	arg2	stabilizer					280:289	an interfacial stabilizer	265:289	an interfacial stabilizer	265:289	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	2	2	used	used	257:260	arg2	CNCs					246:249	CNCs	246:249	CNCs	246:249	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	2	2	used	used	257:260	arg2	nanocrystals					232:243	cellulose nanocrystals	222:243	cellulose nanocrystals (CNCs)	222:250	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	0	3	theme	Emulsions	61:69	arg1	Stability					38:46	the Stability	34:46	the Stability of Pickering Emulsions	34:69	Effect of Oil Phase Transition on the Stability of Pickering Emulsions Stabilized by Cellulose Nanocrystals.					
35171615	7	4	theme	various	1053:1059	arg1	temperatures					1061:1072	various temperatures	1053:1072	various temperatures	1053:1072	Since it displayed similar properties as the emulsifier, it could be used to monitor the CNC coverage on the oil droplets at various temperatures.					
35171615	2	5	theme	oil	327:329	arg1	emulsions					345:353	coconut oil (CO)-in-water emulsions	319:353	coconut oil (CO)-in-water emulsions	319:353	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	0	6	theme	Cellulose	85:93	arg1	Nanocrystals					95:106	Cellulose Nanocrystals	85:106	Cellulose Nanocrystals	85:106	Effect of Oil Phase Transition on the Stability of Pickering Emulsions Stabilized by Cellulose Nanocrystals.					
35171615	0	7	from	Effect	0:5	arg1	Stability					38:46	the Stability	34:46	the Stability of Pickering Emulsions	34:69	Effect of Oil Phase Transition on the Stability of Pickering Emulsions Stabilized by Cellulose Nanocrystals.					
35171615	7	8	theme	oil	1037:1039	arg1	droplets					1041:1048	the oil droplets	1033:1048	the oil droplets at various temperatures	1033:1072	Since it displayed similar properties as the emulsifier, it could be used to monitor the CNC coverage on the oil droplets at various temperatures.					
35171615	1	9	from	strategies	145:154	arg1	engineering					171:181	interfacial engineering	159:181	interfacial engineering for emulsion stability	159:204	Emulsifier design is one of the key strategies in interfacial engineering for emulsion stability.					
35171615	2	10	theme	interfacial	268:278	arg1	stabilizer					280:289	an interfacial stabilizer	265:289	an interfacial stabilizer	265:289	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	2	10	theme	interfacial	268:278	arg1	nanocrystals					232:243	cellulose nanocrystals	222:243	cellulose nanocrystals (CNCs)	222:250	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	8	11	from	interface	1154:1162	arg1	emission					1120:1127	the emission	1116:1127	the emission of PAMAM CNCs at the O/W interface	1116:1162	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	3	12	theme	surface	462:468	arg1	methodology					470:480	the response surface methodology	449:480	the response surface methodology	449:480	A Pickering emulsion consisting of CO and water was optimized based on four parameters using the response surface methodology and the central composite design.					
35171615	4	13	theme	CO	663:664	arg1	temperature					648:658	the melting temperature	636:658	the melting temperature of CO	636:664	The droplet coverage remained stable during the crystallization of the oil phase when the temperature was reduced below the melting temperature of CO.					
35171615	2	14	theme	coconut	319:325	arg1	CO					332:333	CO	332:333	CO	332:333	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	2	14	theme	coconut	319:325	arg1	oil					327:329	coconut oil	319:329	coconut oil (CO)-in-water emulsions	319:353	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	3	15	theme	central	490:496	arg1	design					508:513	the central composite design	486:513	the central composite design	486:513	A Pickering emulsion consisting of CO and water was optimized based on four parameters using the response surface methodology and the central composite design.					
35171615	4	16	theme	melting	640:646	arg1	temperature					648:658	the melting temperature	636:658	the melting temperature of CO	636:664	The droplet coverage remained stable during the crystallization of the oil phase when the temperature was reduced below the melting temperature of CO.					
35171615	5	17	theme	O/W	744:746	arg1	interface					748:756	the O/W interface	740:756	the O/W interface	740:756	Fluorescent-labeled CNCs were used to monitor the partitioning of CNC at the O/W interface during the crystallization of CO.					
35171615	3	18	theme	composite	498:506	arg1	design					508:513	the central composite design	486:513	the central composite design	486:513	A Pickering emulsion consisting of CO and water was optimized based on four parameters using the response surface methodology and the central composite design.					
35171615	1	19	theme	interfacial	159:169	arg1	engineering					171:181	interfacial engineering	159:181	interfacial engineering for emulsion stability	159:204	Emulsifier design is one of the key strategies in interfacial engineering for emulsion stability.					
35171615	7	20	theme	CNC	1017:1019	arg1	coverage					1021:1028	the CNC coverage	1013:1028	the CNC coverage on the oil droplets at various temperatures	1013:1072	Since it displayed similar properties as the emulsifier, it could be used to monitor the CNC coverage on the oil droplets at various temperatures.					
35171615	2	21	theme	emulsions	345:353	arg1	stability					306:314	the stability	302:314	the stability of coconut oil (CO)-in-water emulsions	302:353	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	7	22	used	used	997:1000	arg2	it					985:986	it	985:986	it	985:986	Since it displayed similar properties as the emulsifier, it could be used to monitor the CNC coverage on the oil droplets at various temperatures.					
35171615	4	23	theme	droplet	520:526	arg1	coverage					528:535	The droplet coverage	516:535	The droplet coverage	516:535	The droplet coverage remained stable during the crystallization of the oil phase when the temperature was reduced below the melting temperature of CO.					
35171615	8	24	dep	both	1176:1179	arg1	liquid					1185:1190	liquid	1185:1190	liquid	1185:1190	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	8	25	theme	CNC	1282:1284	arg1	coverage					1286:1293	the fluorescent CNC coverage	1266:1293	the fluorescent CNC coverage on the oil droplets	1266:1313	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	2	26	theme	-in-water	335:343	arg1	emulsions					345:353	coconut oil (CO)-in-water emulsions	319:353	coconut oil (CO)-in-water emulsions	319:353	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	0	27	theme	Phase	14:18	arg1	Transition					20:29	Oil Phase Transition	10:29	Oil Phase Transition	10:29	Effect of Oil Phase Transition on the Stability of Pickering Emulsions Stabilized by Cellulose Nanocrystals.					
35171615	7	28	from	coverage	1021:1028	arg1	droplets					1041:1048	the oil droplets	1033:1048	the oil droplets at various temperatures	1033:1072	Since it displayed similar properties as the emulsifier, it could be used to monitor the CNC coverage on the oil droplets at various temperatures.					
35171615	5	29	theme	Fluorescent-labeled	667:685	arg1	CNCs					687:690	Fluorescent-labeled CNCs	667:690	Fluorescent-labeled CNCs	667:690	Fluorescent-labeled CNCs were used to monitor the partitioning of CNC at the O/W interface during the crystallization of CO.					
35171615	8	30	theme	CNCs	1138:1141	arg1	emission					1120:1127	the emission	1116:1127	the emission of PAMAM CNCs at the O/W interface	1116:1162	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	8	31	theme	oil	1302:1304	arg1	droplets					1306:1313	the oil droplets	1298:1313	the oil droplets	1298:1313	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	1	32	theme	emulsion	187:194	arg1	stability					196:204	emulsion stability	187:204	emulsion stability	187:204	Emulsifier design is one of the key strategies in interfacial engineering for emulsion stability.					
35171615	0	33	theme	Oil	10:12	arg1	Transition					20:29	Oil Phase Transition	10:29	Oil Phase Transition	10:29	Effect of Oil Phase Transition on the Stability of Pickering Emulsions Stabilized by Cellulose Nanocrystals.					
35171615	6	34	theme	fluorescent	911:921	arg1	dendrimer					835:843	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer	792:843	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC	792:884	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC was used as an intrinsic fluorescent dye.					
35171615	6	34	theme	fluorescent	911:921	arg1	dye					923:925	an intrinsic fluorescent dye	898:925	an intrinsic fluorescent dye	898:925	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC was used as an intrinsic fluorescent dye.					
35171615	6	35	used	used	890:893	arg2	dendrimer					835:843	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer	792:843	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC	792:884	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC was used as an intrinsic fluorescent dye.					
35171615	6	35	used	used	890:893	arg2	dye					923:925	an intrinsic fluorescent dye	898:925	an intrinsic fluorescent dye	898:925	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC was used as an intrinsic fluorescent dye.					
35171615	1	36	theme	strategies	145:154	arg1	one					130:132	one	130:132	one	130:132	Emulsifier design is one of the key strategies in interfacial engineering for emulsion stability.					
35171615	1	36	theme	strategies	145:154	arg1	strategies					145:154	the key strategies	137:154	the key strategies in interfacial engineering for emulsion stability	137:204	Emulsifier design is one of the key strategies in interfacial engineering for emulsion stability.					
35171615	5	37	used	used	697:700	arg2	CNCs					687:690	Fluorescent-labeled CNCs	667:690	Fluorescent-labeled CNCs	667:690	Fluorescent-labeled CNCs were used to monitor the partitioning of CNC at the O/W interface during the crystallization of CO.					
35171615	6	38	theme	intrinsic	901:909	arg1	dendrimer					835:843	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer	792:843	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC	792:884	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC was used as an intrinsic fluorescent dye.					
35171615	6	38	theme	intrinsic	901:909	arg1	dye					923:925	an intrinsic fluorescent dye	898:925	an intrinsic fluorescent dye	898:925	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC was used as an intrinsic fluorescent dye.					
35171615	2	39	theme	cellulose	222:230	arg1	stabilizer					280:289	an interfacial stabilizer	265:289	an interfacial stabilizer	265:289	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	2	39	theme	cellulose	222:230	arg1	CNCs					246:249	CNCs	246:249	CNCs	246:249	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	2	39	theme	cellulose	222:230	arg1	nanocrystals					232:243	cellulose nanocrystals	222:243	cellulose nanocrystals (CNCs)	222:250	In this study, cellulose nanocrystals (CNCs) were used as an interfacial stabilizer to improve the stability of coconut oil (CO)-in-water emulsions.					
35171615	8	40	theme	CO	1202:1203	arg1	droplets					1205:1212	both the liquid and solid CO droplets	1176:1212	both the liquid and solid CO droplets	1176:1212	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	0	41	theme	Transition	20:29	arg1	Effect					0:5	Effect	0:5	Effect of Oil Phase Transition on the Stability of Pickering Emulsions	0:69	Effect of Oil Phase Transition on the Stability of Pickering Emulsions Stabilized by Cellulose Nanocrystals.					
35171615	5	42	theme	CO	788:789	arg1	crystallization					769:783	the crystallization	765:783	the crystallization of CO	765:789	Fluorescent-labeled CNCs were used to monitor the partitioning of CNC at the O/W interface during the crystallization of CO.					
35171615	3	43	theme	Pickering	358:366	arg1	emulsion					368:375	A Pickering emulsion	356:375	A Pickering emulsion consisting of CO and water	356:402	A Pickering emulsion consisting of CO and water was optimized based on four parameters using the response surface methodology and the central composite design.					
35171615	1	44	theme	Emulsifier	109:118	arg1	design					120:125	Emulsifier design	109:125	Emulsifier design	109:125	Emulsifier design is one of the key strategies in interfacial engineering for emulsion stability.					
35171615	8	45	theme	O/W	1150:1152	arg1	interface					1154:1162	the O/W interface	1146:1162	the O/W interface	1146:1162	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	7	46	from	temperatures	1061:1072	arg1	droplets					1041:1048	the oil droplets	1033:1048	the oil droplets at various temperatures	1033:1072	Since it displayed similar properties as the emulsifier, it could be used to monitor the CNC coverage on the oil droplets at various temperatures.					
35171615	6	47	theme	polyamidoamine	809:822	arg1	dendrimer					835:843	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer	792:843	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC	792:884	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC was used as an intrinsic fluorescent dye.					
35171615	6	47	theme	polyamidoamine	809:822	arg1	dye					923:925	an intrinsic fluorescent dye	898:925	an intrinsic fluorescent dye	898:925	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC was used as an intrinsic fluorescent dye.					
35171615	3	48	theme	response	453:460	arg1	methodology					470:480	the response surface methodology	449:480	the response surface methodology	449:480	A Pickering emulsion consisting of CO and water was optimized based on four parameters using the response surface methodology and the central composite design.					
35171615	8	49	dep	droplets	1205:1212	arg1	both					1176:1179	both	1176:1179	both	1176:1179	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	8	49	dep	droplets	1205:1212	arg1	solid					1196:1200	solid	1196:1200	solid	1196:1200	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	8	50	theme	fluorescent	1270:1280	arg1	coverage					1286:1293	the fluorescent CNC coverage	1266:1293	the fluorescent CNC coverage on the oil droplets	1266:1313	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	6	51	theme	Generation	796:805	arg1	polyamidoamine					809:822	Generation 6 polyamidoamine	796:822	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC	792:884	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC was used as an intrinsic fluorescent dye.					
35171615	6	51	theme	Generation	796:805	arg1	PAMAM					828:832	PAMAM	828:832	PAMAM	828:832	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC was used as an intrinsic fluorescent dye.					
35171615	0	52	theme	Pickering	51:59	arg1	Emulsions					61:69	Pickering Emulsions	51:69	Pickering Emulsions	51:69	Effect of Oil Phase Transition on the Stability of Pickering Emulsions Stabilized by Cellulose Nanocrystals.					
35171615	8	53	from	coverage	1286:1293	arg1	droplets					1306:1313	the oil droplets	1298:1313	the oil droplets	1298:1313	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	7	54	theme	similar	947:953	arg1	properties					955:964	similar properties	947:964	similar properties	947:964	Since it displayed similar properties as the emulsifier, it could be used to monitor the CNC coverage on the oil droplets at various temperatures.					
35171615	7	54	theme	similar	947:953	arg1	emulsifier					973:982	the emulsifier	969:982	the emulsifier	969:982	Since it displayed similar properties as the emulsifier, it could be used to monitor the CNC coverage on the oil droplets at various temperatures.					
35171615	4	55	theme	phase	591:595	arg1	crystallization					564:578	the crystallization	560:578	the crystallization of the oil phase	560:595	The droplet coverage remained stable during the crystallization of the oil phase when the temperature was reduced below the melting temperature of CO.					
35171615	5	56	theme	CNC	733:735	arg1	partitioning					717:728	the partitioning	713:728	the partitioning of CNC	713:735	Fluorescent-labeled CNCs were used to monitor the partitioning of CNC at the O/W interface during the crystallization of CO.					
35171615	6	57	theme	CNC	882:884	arg1	surface					871:877	the surface	867:877	the surface of CNC	867:884	The Generation 6 polyamidoamine (G6 PAMAM) dendrimer covalently grafted on the surface of CNC was used as an intrinsic fluorescent dye.					
35171615	8	58	theme	PAMAM	1132:1136	arg1	CNCs					1138:1141	PAMAM CNCs	1132:1141	PAMAM CNCs	1132:1141	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	8	59	theme	oil	1231:1233	arg1	crystallization					1235:1249	oil crystallization	1231:1249	oil crystallization	1231:1249	The fluorescence micrographs showed that the emission of PAMAM CNCs at the O/W interface remained on both the liquid and solid CO droplets, confirming that oil crystallization did not affect the fluorescent CNC coverage on the oil droplets.					
35171615	4	60	theme	oil	587:589	arg1	phase					591:595	the oil phase	583:595	the oil phase	583:595	The droplet coverage remained stable during the crystallization of the oil phase when the temperature was reduced below the melting temperature of CO.					
36793729	0	0	theme	white	75:79	arg1	shrimp					81:86	white shrimp	75:86	white shrimp (Litopenaeus vannamei)	75:109	Dietary supplementation of β-1, 3-glucan improves the intestinal health of white shrimp (Litopenaeus vannamei) by modulating intestinal microbiota and inhibiting inflammatory response.					
36793729	5	1	theme	shrimp	1604:1609	arg1	growth					1594:1599	the growth	1590:1599	the growth of shrimp fed β-1,3-glucan	1590:1626	The improvement of intestinal health referred to the elevation in intestinal immune and antioxidant capacity, ultimately contributing to the growth of shrimp fed β-1,3-glucan.					
36793729	6	2	theme	shrimp	1727:1732	arg1	health					1711:1716	the intestinal health	1696:1716	the intestinal health of white shrimp	1696:1732	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	6	3	theme	capacity	1892:1899	arg1	suppression					1799:1809	the suppression	1795:1809	the suppression of intestinal inflammatory response	1795:1845	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	6	3	theme	capacity	1892:1899	arg1	elevation					1856:1864	the elevation	1852:1864	the elevation of immune and antioxidant capacity	1852:1899	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	6	3	theme	capacity	1892:1899	arg1	modulation					1746:1755	the modulation	1742:1755	the modulation of intestinal microbiota homeostasis	1742:1792	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	1	4	theme	shrimp	254:259	arg1	culture					284:290	white shrimp (Litopenaeus vannamei) culture	248:290	white shrimp (Litopenaeus vannamei) culture	248:290	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	2	5	theme	predominant	483:493	arg1	Actinobacteria					454:467	Actinobacteria	454:467	Actinobacteria	454:467	Proteobacteria, Firmicutes, and Actinobacteria served as the predominant phyla inhabiting the intestine of white shrimp, whilst a significant variation in their proportion was recorded in shrimp fed with basal and β-1,3-glucan supplementation diets in this study.					
36793729	2	5	theme	predominant	483:493	arg1	phyla					495:499	the predominant phyla	479:499	the predominant phyla inhabiting the intestine of white shrimp	479:540	Proteobacteria, Firmicutes, and Actinobacteria served as the predominant phyla inhabiting the intestine of white shrimp, whilst a significant variation in their proportion was recorded in shrimp fed with basal and β-1,3-glucan supplementation diets in this study.					
36793729	2	5	theme	predominant	483:493	arg1	Firmicutes					438:447	Firmicutes	438:447	Firmicutes	438:447	Proteobacteria, Firmicutes, and Actinobacteria served as the predominant phyla inhabiting the intestine of white shrimp, whilst a significant variation in their proportion was recorded in shrimp fed with basal and β-1,3-glucan supplementation diets in this study.					
36793729	2	5	theme	predominant	483:493	arg1	Proteobacteria					422:435	Proteobacteria	422:435	Proteobacteria	422:435	Proteobacteria, Firmicutes, and Actinobacteria served as the predominant phyla inhabiting the intestine of white shrimp, whilst a significant variation in their proportion was recorded in shrimp fed with basal and β-1,3-glucan supplementation diets in this study.					
36793729	6	6	theme	inflammatory	1825:1836	arg1	response					1838:1845	intestinal inflammatory response	1814:1845	intestinal inflammatory response	1814:1845	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	2	7	from	variation	564:572	arg1	proportion					583:592	their proportion	577:592	their proportion	577:592	Proteobacteria, Firmicutes, and Actinobacteria served as the predominant phyla inhabiting the intestine of white shrimp, whilst a significant variation in their proportion was recorded in shrimp fed with basal and β-1,3-glucan supplementation diets in this study.					
36793729	2	8	theme	shrimp	535:540	arg1	intestine					516:524	the intestine	512:524	the intestine of white shrimp	512:540	Proteobacteria, Firmicutes, and Actinobacteria served as the predominant phyla inhabiting the intestine of white shrimp, whilst a significant variation in their proportion was recorded in shrimp fed with basal and β-1,3-glucan supplementation diets in this study.					
36793729	6	9	theme	microbiota	1771:1780	arg1	homeostasis					1782:1792	intestinal microbiota homeostasis	1760:1792	intestinal microbiota homeostasis	1760:1792	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	6	10	theme	antioxidant	1880:1890	arg1	capacity					1892:1899	immune and antioxidant capacity	1869:1899	capacity	1892:1899	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	3	11	with	concurrent	812:821	arg1	reduction					838:846	a notable reduction	828:846	a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes	828:919	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	6	12	theme	intestinal	1700:1709	arg1	health					1711:1716	the intestinal health	1696:1716	the intestinal health of white shrimp	1696:1732	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	0	13	theme	shrimp	81:86	arg1	health					65:70	the intestinal health	50:70	the intestinal health of white shrimp (Litopenaeus vannamei)	50:109	Dietary supplementation of β-1, 3-glucan improves the intestinal health of white shrimp (Litopenaeus vannamei) by modulating intestinal microbiota and inhibiting inflammatory response.					
36793729	1	14	theme	intestinal	349:358	arg1	health					360:365	intestinal health	349:365	intestinal health with a lack understanding of its underlying mechanism	349:419	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	2	15	theme	supplementation	649:663	arg1	diets					665:669	basal and β-1,3-glucan supplementation diets	626:669	basal and β-1,3-glucan supplementation diets	626:669	Proteobacteria, Firmicutes, and Actinobacteria served as the predominant phyla inhabiting the intestine of white shrimp, whilst a significant variation in their proportion was recorded in shrimp fed with basal and β-1,3-glucan supplementation diets in this study.					
36793729	6	16	theme	β-1,3-glucan	1658:1669	arg1	supplementation					1671:1685	β-1,3-glucan supplementation	1658:1685	β-1,3-glucan supplementation	1658:1685	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	3	17	theme	microbial	758:766	arg1	diversity					768:776	the microbial diversity	754:776	the microbial diversity	754:776	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	2	18	theme	β-1,3-glucan	636:647	arg1	diets					665:669	basal and β-1,3-glucan supplementation diets	626:669	basal and β-1,3-glucan supplementation diets	626:669	Proteobacteria, Firmicutes, and Actinobacteria served as the predominant phyla inhabiting the intestine of white shrimp, whilst a significant variation in their proportion was recorded in shrimp fed with basal and β-1,3-glucan supplementation diets in this study.					
36793729	3	19	theme	basal	963:967	arg1	group					974:978	the basal diet group	959:978	the basal diet group	959:978	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	4	20	theme	microbial	998:1006	arg1	diversity					1008:1016	microbial diversity	998:1016	microbial diversity	998:1016	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	4	21	theme	inflammatory	1430:1441	arg1	response					1443:1450	the intestinal inflammatory response	1415:1450	the intestinal inflammatory response	1415:1450	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	2	22	theme	basal	626:630	arg1	diets					665:669	basal and β-1,3-glucan supplementation diets	626:669	basal and β-1,3-glucan supplementation diets	626:669	Proteobacteria, Firmicutes, and Actinobacteria served as the predominant phyla inhabiting the intestine of white shrimp, whilst a significant variation in their proportion was recorded in shrimp fed with basal and β-1,3-glucan supplementation diets in this study.					
36793729	3	23	dep	microbes	912:919	arg1	Aeromonas					887:895	Aeromonas	887:895	Aeromonas	887:895	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	0	24	dep	shrimp	81:86	arg1	vannamei					101:108	Litopenaeus vannamei	89:108	Litopenaeus vannamei	89:108	Dietary supplementation of β-1, 3-glucan improves the intestinal health of white shrimp (Litopenaeus vannamei) by modulating intestinal microbiota and inhibiting inflammatory response.					
36793729	4	25	theme	microbiota	1089:1098	arg1	homeostasis					1063:1073	the homeostasis	1059:1073	the homeostasis of intestinal microbiota	1059:1098	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	4	26	theme	intestinal	1419:1428	arg1	response					1443:1450	the intestinal inflammatory response	1415:1450	the intestinal inflammatory response	1415:1450	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	4	27	theme	β-1,3-glucan	1270:1281	arg1	diet					1283:1286	β-1,3-glucan diet	1270:1286	β-1,3-glucan diet	1270:1286	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	4	28	theme	ecological	1207:1216	arg1	networks					1218:1225	ecological networks	1207:1225	ecological networks	1207:1225	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	0	29	theme	Dietary	0:6	arg1	supplementation					8:22	Dietary supplementation	0:22	Dietary supplementation of β-1, 3-glucan	0:39	Dietary supplementation of β-1, 3-glucan improves the intestinal health of white shrimp (Litopenaeus vannamei) by modulating intestinal microbiota and inhibiting inflammatory response.					
36793729	4	30	theme	lipopolysaccharide	1344:1361	arg1	biosynthesis					1363:1374	lipopolysaccharide biosynthesis	1344:1374	lipopolysaccharide biosynthesis	1344:1374	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	6	31	theme	intestinal	1760:1769	arg1	homeostasis					1782:1792	intestinal microbiota homeostasis	1760:1792	intestinal microbiota homeostasis	1760:1792	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	4	32	theme	intestinal	1078:1087	arg1	microbiota					1089:1098	intestinal microbiota	1078:1098	intestinal microbiota	1078:1098	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	4	33	theme	competition	1172:1182	arg1	increase					1112:1119	the increase	1108:1119	the increase of specialists' number	1108:1142	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	4	33	theme	competition	1172:1182	arg1	inhibition					1148:1157	inhibition	1148:1157	inhibition of microbial competition caused by Aeromonas in ecological networks	1148:1225	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	4	34	from	Aeromonas	1194:1202	arg1	networks					1218:1225	ecological networks	1207:1225	ecological networks	1207:1225	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	0	35	theme	β-1	27:29	arg1	supplementation					8:22	Dietary supplementation	0:22	Dietary supplementation of β-1, 3-glucan	0:39	Dietary supplementation of β-1, 3-glucan improves the intestinal health of white shrimp (Litopenaeus vannamei) by modulating intestinal microbiota and inhibiting inflammatory response.					
36793729	3	36	theme	microbial	789:797	arg1	composition					799:809	microbial composition	789:809	microbial composition	789:809	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	2	37	theme	significant	552:562	arg1	variation					564:572	a significant variation	550:572	a significant variation in their proportion	550:592	Proteobacteria, Firmicutes, and Actinobacteria served as the predominant phyla inhabiting the intestine of white shrimp, whilst a significant variation in their proportion was recorded in shrimp fed with basal and β-1,3-glucan supplementation diets in this study.					
36793729	4	38	theme	related	1333:1339	arg1	metabolism					1322:1331	microbial metabolism	1312:1331	microbial metabolism related to lipopolysaccharide biosynthesis	1312:1374	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	4	39	theme	microbial	1162:1170	arg1	competition					1172:1182	microbial competition	1162:1182	microbial competition caused by Aeromonas in ecological networks	1162:1225	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	5	40	from	elevation	1506:1514	arg1	immune					1530:1535	immune	1530:1535	immune	1530:1535	The improvement of intestinal health referred to the elevation in intestinal immune and antioxidant capacity, ultimately contributing to the growth of shrimp fed β-1,3-glucan.					
36793729	5	40	from	elevation	1506:1514	arg1	capacity					1553:1560	intestinal immune and antioxidant capacity	1519:1560	capacity	1553:1560	The improvement of intestinal health referred to the elevation in intestinal immune and antioxidant capacity, ultimately contributing to the growth of shrimp fed β-1,3-glucan.					
36793729	1	41	from	health	360:365	arg1	beneficial					335:344	beneficial	335:344	beneficial	335:344	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	1	42	theme	lack	374:377	arg1	understanding					379:391	a lack understanding	372:391	a lack understanding of its underlying mechanism	372:419	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	3	43	theme	diet	969:972	arg1	group					974:978	the basal diet group	959:978	the basal diet group	959:978	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	1	44	theme	intestinal	203:212	arg1	dysfunction					214:224	intestinal dysfunction	203:224	intestinal dysfunction	203:224	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	1	45	from	beneficial	335:344	arg1	health					360:365	intestinal health	349:365	intestinal health with a lack understanding of its underlying mechanism	349:419	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	6	46	theme	shrimp	1948:1953	arg1	growth					1932:1937	the growth	1928:1937	the growth of white shrimp	1928:1953	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	0	47	theme	intestinal	125:134	arg1	microbiota					136:145	intestinal microbiota	125:145	intestinal microbiota	125:145	Dietary supplementation of β-1, 3-glucan improves the intestinal health of white shrimp (Litopenaeus vannamei) by modulating intestinal microbiota and inhibiting inflammatory response.					
36793729	1	48	located	observed	236:243	arg2	phenomenon					189:198	The phenomenon	185:198	The phenomenon of intestinal dysfunction	185:224	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	1	48	located	observed	236:243	arg1	culture					284:290	white shrimp (Litopenaeus vannamei) culture	248:290	white shrimp (Litopenaeus vannamei) culture	248:290	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	6	49	theme	immune	1869:1874	arg1	capacity					1892:1899	immune and antioxidant capacity	1869:1899	capacity	1892:1899	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	1	50	theme	dysfunction	214:224	arg1	phenomenon					189:198	The phenomenon	185:198	The phenomenon of intestinal dysfunction	185:224	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	3	51	theme	concurrent	812:821	arg1	composition					799:809	microbial composition	789:809	microbial composition	789:809	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	3	52	theme	opportunistic	864:876	arg1	pathogen					878:885	opportunistic pathogen	864:885	opportunistic pathogen	864:885	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	6	53	theme	response	1838:1845	arg1	suppression					1799:1809	the suppression	1795:1809	the suppression of intestinal inflammatory response	1795:1845	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	6	53	theme	response	1838:1845	arg1	elevation					1856:1864	the elevation	1852:1864	the elevation of immune and antioxidant capacity	1852:1899	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	6	53	theme	response	1838:1845	arg1	modulation					1746:1755	the modulation	1742:1755	the modulation of intestinal microbiota homeostasis	1742:1792	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	6	54	theme	white	1942:1946	arg1	shrimp					1948:1953	white shrimp	1942:1953	white shrimp	1942:1953	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	4	55	theme	conspicuous	1391:1401	arg1	decrease					1403:1410	a conspicuous decrease	1389:1410	a conspicuous decrease in the intestinal inflammatory response	1389:1450	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	2	56	theme	white	529:533	arg1	shrimp					535:540	white shrimp	529:540	white shrimp	529:540	Proteobacteria, Firmicutes, and Actinobacteria served as the predominant phyla inhabiting the intestine of white shrimp, whilst a significant variation in their proportion was recorded in shrimp fed with basal and β-1,3-glucan supplementation diets in this study.					
36793729	3	57	from	reduction	838:846	arg1	ratio					855:859	the ratio	851:859	the ratio of opportunistic pathogen Aeromonas, gram-negative microbes	851:919	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	4	58	from	decrease	1403:1410	arg1	response					1443:1450	the intestinal inflammatory response	1415:1450	the intestinal inflammatory response	1415:1450	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	1	59	theme	underlying	400:409	arg1	mechanism					411:419	its underlying mechanism	396:419	its underlying mechanism	396:419	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	3	60	theme	Dietary	686:692	arg1	supplementation					694:708	Dietary supplementation	686:708	Dietary supplementation of β-1,3-glucan	686:724	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	5	61	theme	antioxidant	1541:1551	arg1	capacity					1553:1560	intestinal immune and antioxidant capacity	1519:1560	capacity	1553:1560	The improvement of intestinal health referred to the elevation in intestinal immune and antioxidant capacity, ultimately contributing to the growth of shrimp fed β-1,3-glucan.					
36793729	0	62	theme	inflammatory	162:173	arg1	response					175:182	inflammatory response	162:182	inflammatory response	162:182	Dietary supplementation of β-1, 3-glucan improves the intestinal health of white shrimp (Litopenaeus vannamei) by modulating intestinal microbiota and inhibiting inflammatory response.					
36793729	1	63	theme	mechanism	411:419	arg1	understanding					379:391	a lack understanding	372:391	a lack understanding of its underlying mechanism	372:419	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	4	64	theme	Aeromonas	1257:1265	arg1	inhibition					1243:1252	the inhibition	1239:1252	the inhibition of Aeromonas by β-1,3-glucan diet	1239:1286	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	3	65	theme	gram-negative	898:910	arg1	microbes					912:919	gram-negative microbes	898:919	gram-negative microbes	898:919	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	3	66	from	Gammaproteobacteria	927:945	arg1	composition					799:809	microbial composition	789:809	microbial composition	789:809	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	1	67	dep	shrimp	254:259	arg1	vannamei					274:281	Litopenaeus vannamei	262:281	Litopenaeus vannamei	262:281	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	6	68	theme	white	1721:1725	arg1	shrimp					1727:1732	white shrimp	1721:1732	white shrimp	1721:1732	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	6	69	theme	homeostasis	1782:1792	arg1	suppression					1799:1809	the suppression	1795:1809	the suppression of intestinal inflammatory response	1795:1845	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	6	69	theme	homeostasis	1782:1792	arg1	elevation					1856:1864	the elevation	1852:1864	the elevation of immune and antioxidant capacity	1852:1899	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	6	69	theme	homeostasis	1782:1792	arg1	modulation					1746:1755	the modulation	1742:1755	the modulation of intestinal microbiota homeostasis	1742:1792	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	3	70	theme	pathogen	878:885	arg1	ratio					855:859	the ratio	851:859	the ratio of opportunistic pathogen Aeromonas, gram-negative microbes	851:919	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	6	71	theme	intestinal	1814:1823	arg1	response					1838:1845	intestinal inflammatory response	1814:1845	intestinal inflammatory response	1814:1845	These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.					
36793729	5	72	theme	intestinal	1472:1481	arg1	health					1483:1488	intestinal health	1472:1488	intestinal health	1472:1488	The improvement of intestinal health referred to the elevation in intestinal immune and antioxidant capacity, ultimately contributing to the growth of shrimp fed β-1,3-glucan.					
36793729	1	73	with	health	360:365	arg1	understanding					379:391	a lack understanding	372:391	a lack understanding of its underlying mechanism	372:419	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
36793729	4	74	theme	microbial	1312:1320	arg1	metabolism					1322:1331	microbial metabolism	1312:1331	microbial metabolism related to lipopolysaccharide biosynthesis	1312:1374	The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists' number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response.					
36793729	0	75	theme	intestinal	54:63	arg1	health					65:70	the intestinal health	50:70	the intestinal health of white shrimp (Litopenaeus vannamei)	50:109	Dietary supplementation of β-1, 3-glucan improves the intestinal health of white shrimp (Litopenaeus vannamei) by modulating intestinal microbiota and inhibiting inflammatory response.					
36793729	3	76	theme	β-1,3-glucan	713:724	arg1	supplementation					694:708	Dietary supplementation	686:708	Dietary supplementation of β-1,3-glucan	686:724	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	3	77	theme	notable	830:836	arg1	reduction					838:846	a notable reduction	828:846	a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes	828:919	Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group.					
36793729	5	78	theme	health	1483:1488	arg1	improvement					1457:1467	The improvement	1453:1467	The improvement of intestinal health	1453:1488	The improvement of intestinal health referred to the elevation in intestinal immune and antioxidant capacity, ultimately contributing to the growth of shrimp fed β-1,3-glucan.					
36793729	1	79	theme	white	248:252	arg1	shrimp					254:259	white shrimp	248:259	white shrimp (Litopenaeus vannamei) culture	248:290	The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism.					
34718693	5	0	dep	METHODS	772:778	arg1	based					794:798	based	794:798	was based on the NutriNet-Santé cohort	790:827	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	5	0	dep	METHODS	772:778	arg1	included					901:908	included	901:908	were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y)	896:1010	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	6	1	theme	Baseline	1013:1020	arg1	intakes					1030:1036	Baseline dietary intakes	1013:1036	Baseline dietary intakes	1013:1036	Baseline dietary intakes were obtained from repeated 24-h dietary records linked to a detailed food composition table.					
34718693	10	2	theme	French	1870:1875	arg1	adults					1877:1882	French adults	1870:1882	French adults from the NutriNet-Santé cohort	1870:1913	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	5	3	dep	age	981:983	arg1	y					1009:1009	42.1 ± 14.5 y	997:1009	mean ± SD age at baseline 42.1 ± 14.5 y	971:1009	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	6	4	theme	food	1108:1111	arg1	table					1125:1129	a detailed food composition table	1097:1129	a detailed food composition table	1097:1129	Baseline dietary intakes were obtained from repeated 24-h dietary records linked to a detailed food composition table.					
34718693	8	5	dep	risk	1422:1425	arg1	HR					1453:1454	HR	1453:1454	HR for sex-specific Q5 compared with Q1	1453:1491	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	8	5	dep	risk	1422:1425	arg1	cases					1446:1450	n = 3374 incident cases	1428:1450	n = 3374 incident cases	1428:1450	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	7	6	theme	variables	1338:1346	arg1	range					1281:1285	a large range	1273:1285	a large range of lifestyle, sociodemographic, and anthropometric variables	1273:1346	Associations between FODMAP intake (expressed in quintiles, Q) and cancer risks were assessed by Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables.					
34718693	10	7	theme	NutriNet-Santé	1893:1906	arg1	cohort					1908:1913	the NutriNet-Santé cohort	1889:1913	the NutriNet-Santé cohort	1889:1913	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	3	8	theme	epidemiologic	517:529	arg1	study					531:535	no epidemiologic study	514:535	no epidemiologic study	514:535	However, to our knowledge, no epidemiologic study has investigated this hypothesis so far.					
34718693	5	9	theme	NutriNet-Santé	807:820	arg1	cohort					822:827	the NutriNet-Santé cohort	803:827	the NutriNet-Santé cohort	803:827	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	9	10	dep	%	1774:1774	arg1	P-trend = 0.02					1791:1804	P-trend = 0.02	1791:1804	95% CI: 1.13-2.79; P-trend = 0.02	1772:1804	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	7	11	theme	sociodemographic	1301:1316	arg1	range					1281:1285	a large range	1273:1285	a large range of lifestyle, sociodemographic, and anthropometric variables	1273:1346	Associations between FODMAP intake (expressed in quintiles, Q) and cancer risks were assessed by Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables.					
34718693	9	12	theme	HR	1742:1743	arg1	Q5					1745:1746	HR Q5	1742:1746	HR Q5 compared with Q1: 1.78	1742:1769	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	4	13	from	associations	626:637	arg1	cohort					764:769	a large prospective cohort	744:769	a large prospective cohort	744:769	OBJECTIVES Our objective was to investigate the associations between FODMAP intake (total and by type) and cancer risk (overall, breast, prostate, and colorectal) in a large prospective cohort.					
34718693	9	14	theme	cancer	1590:1595	arg1	risk					1597:1600	cancer risk	1590:1600	cancer risk	1590:1600	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	8	15	theme	FODMAP	1363:1368	arg1	intake					1370:1375	RESULTS Total FODMAP intake	1349:1375	RESULTS Total FODMAP intake	1349:1375	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	4	16	theme	FODMAP	647:652	arg1	intake					654:659	FODMAP intake	647:659	FODMAP intake (total and by type)	647:679	OBJECTIVES Our objective was to investigate the associations between FODMAP intake (total and by type) and cancer risk (overall, breast, prostate, and colorectal) in a large prospective cohort.					
34718693	0	17	theme	NutriNet-Santé	119:132	arg1	Cohort					134:139	the Prospective NutriNet-Santé Cohort	103:139	the Prospective NutriNet-Santé Cohort	103:139	Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols (FODMAPs) and Cancer Risk in the Prospective NutriNet-Santé Cohort.					
34718693	11	18	from	data	2099:2102	arg1	mechanisms					2132:2141	the potential underlying mechanisms	2107:2141	the potential underlying mechanisms	2107:2141	Further epidemiologic and experimental studies are needed to confirm these results and provide data on the potential underlying mechanisms.					
34718693	11	19	theme	Further	2004:2010	arg1	studies					2043:2049	Further epidemiologic and experimental studies	2004:2049	Further epidemiologic and experimental studies	2004:2049	Further epidemiologic and experimental studies are needed to confirm these results and provide data on the potential underlying mechanisms.					
34718693	8	20	theme	overall	1407:1413	arg1	risk					1422:1425	increased overall cancer risk	1397:1425	increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04)	1397:1534	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	1	21	theme	BACKGROUND	142:151	arg1	FODMAPs					228:234	FODMAPs	228:234	FODMAPs	228:234	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) have been shown to be involved in gastrointestinal disorders.					
34718693	1	21	theme	BACKGROUND	142:151	arg1	oligosaccharides					165:180	BACKGROUND Fermentable oligosaccharides	142:180	BACKGROUND Fermentable oligosaccharides	142:180	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) have been shown to be involved in gastrointestinal disorders.					
34718693	9	22	theme	overall	1628:1634	arg1	cancer					1636:1641	overall cancer	1628:1641	overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04)	1628:1708	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	9	22	theme	overall	1628:1634	arg1	Q5					1647:1648	HR Q5	1644:1648	HR Q5 compared with Q1	1644:1665	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	7	23	theme	FODMAP	1153:1158	arg1	intake					1160:1165	FODMAP intake	1153:1165	FODMAP intake (expressed in quintiles, Q)	1153:1193	Associations between FODMAP intake (expressed in quintiles, Q) and cancer risks were assessed by Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables.					
34718693	10	24	theme	development	1991:2001	arg1	intake					1961:1966	FODMAP intake	1954:1966	FODMAP intake	1954:1966	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	10	24	theme	development	1991:2001	arg1	risk					1976:1979	the risk	1972:1979	the risk of cancer development	1972:2001	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	4	25	theme	prospective	752:762	arg1	cohort					764:769	a large prospective cohort	744:769	a large prospective cohort	744:769	OBJECTIVES Our objective was to investigate the associations between FODMAP intake (total and by type) and cancer risk (overall, breast, prostate, and colorectal) in a large prospective cohort.					
34718693	0	26	theme	Fermentable	0:10	arg1	FODMAPs					75:81	FODMAPs	75:81	FODMAPs	75:81	Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols (FODMAPs) and Cancer Risk in the Prospective NutriNet-Santé Cohort.					
34718693	0	26	theme	Fermentable	0:10	arg1	Oligosaccharides					12:27	Fermentable Oligosaccharides	0:27	Fermentable Oligosaccharides	0:27	Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols (FODMAPs) and Cancer Risk in the Prospective NutriNet-Santé Cohort.					
34718693	7	27	theme	proportional	1233:1244	arg1	models					1253:1258	Cox proportional hazard models	1229:1258	Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables	1229:1346	Associations between FODMAP intake (expressed in quintiles, Q) and cancer risks were assessed by Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables.					
34718693	2	28	theme	chronic	434:440	arg1	diseases					442:449	other chronic diseases	428:449	other chronic diseases such as cancer	428:464	In view of their proinflammatory potential and their interactions with the gut microbiota, their contribution to the etiology of other chronic diseases such as cancer has been postulated.					
34718693	2	28	theme	chronic	434:440	arg1	cancer					459:464	cancer	459:464	cancer	459:464	In view of their proinflammatory potential and their interactions with the gut microbiota, their contribution to the etiology of other chronic diseases such as cancer has been postulated.					
34718693	8	29	theme	sex-specific	1460:1471	arg1	Q5					1473:1474	sex-specific Q5	1460:1474	sex-specific Q5 compared with Q1	1460:1491	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	7	30	dep	intake	1160:1165	arg1	expressed					1168:1176	expressed	1168:1176	expressed	1168:1176	Associations between FODMAP intake (expressed in quintiles, Q) and cancer risks were assessed by Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables.					
34718693	10	31	from	cohort	1908:1913	arg1	adults					1877:1882	French adults	1870:1882	French adults from the NutriNet-Santé cohort	1870:1913	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	7	32	theme	cancer	1199:1204	arg1	risks					1206:1210	cancer risks	1199:1210	cancer risks	1199:1210	Associations between FODMAP intake (expressed in quintiles, Q) and cancer risks were assessed by Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables.					
34718693	10	33	theme	population-based	1844:1859	arg1	study					1861:1865	this large population-based study	1833:1865	this large population-based study on French adults from the NutriNet-Santé cohort	1833:1913	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	2	34	theme	potential	332:340	arg1	view					302:305	view	302:305	view of their proinflammatory potential	302:340	In view of their proinflammatory potential and their interactions with the gut microbiota, their contribution to the etiology of other chronic diseases such as cancer has been postulated.					
34718693	2	34	theme	potential	332:340	arg1	interactions					352:363	their interactions	346:363	their interactions with the gut microbiota	346:387	In view of their proinflammatory potential and their interactions with the gut microbiota, their contribution to the etiology of other chronic diseases such as cancer has been postulated.					
34718693	8	35	theme	Total	1357:1361	arg1	intake					1370:1375	RESULTS Total FODMAP intake	1349:1375	RESULTS Total FODMAP intake	1349:1375	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	2	36	theme	gut	374:376	arg1	microbiota					378:387	the gut microbiota	370:387	the gut microbiota	370:387	In view of their proinflammatory potential and their interactions with the gut microbiota, their contribution to the etiology of other chronic diseases such as cancer has been postulated.					
34718693	5	37	from	baseline	887:894	arg1	participants					856:867	104,909 adult participants	842:867	104,909 adult participants without cancer at baseline	842:894	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	4	38	dep	intake	654:659	arg1	total					662:666	total	662:666	total	662:666	OBJECTIVES Our objective was to investigate the associations between FODMAP intake (total and by type) and cancer risk (overall, breast, prostate, and colorectal) in a large prospective cohort.					
34718693	4	38	dep	intake	654:659	arg1	type					675:678	type	675:678	type	675:678	OBJECTIVES Our objective was to investigate the associations between FODMAP intake (total and by type) and cancer risk (overall, breast, prostate, and colorectal) in a large prospective cohort.					
34718693	6	39	theme	dietary	1022:1028	arg1	intakes					1030:1036	Baseline dietary intakes	1013:1036	Baseline dietary intakes	1013:1036	Baseline dietary intakes were obtained from repeated 24-h dietary records linked to a detailed food composition table.					
34718693	6	40	theme	detailed	1099:1106	arg1	table					1125:1129	a detailed food composition table	1097:1129	a detailed food composition table	1097:1129	Baseline dietary intakes were obtained from repeated 24-h dietary records linked to a detailed food composition table.					
34718693	9	41	theme	colorectal	1714:1723	arg1	cancer					1725:1730	colorectal cancer	1714:1730	colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02)	1714:1805	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	8	42	theme	1.44	1514:1517	arg1	%					1502:1502	95% CI: 1.02	1500:1511	95% CI: 1.02	1500:1511	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	9	43	dep	Q1	1762:1763	arg1	1.78					1766:1769	1.78	1766:1769	1.78	1766:1769	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	7	44	theme	anthropometric	1323:1336	arg1	variables					1338:1346	lifestyle, sociodemographic, and anthropometric variables	1290:1346	variables	1338:1346	Associations between FODMAP intake (expressed in quintiles, Q) and cancer risks were assessed by Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables.					
34718693	0	45	theme	Prospective	107:117	arg1	Cohort					134:139	the Prospective NutriNet-Santé Cohort	103:139	the Prospective NutriNet-Santé Cohort	103:139	Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols (FODMAPs) and Cancer Risk in the Prospective NutriNet-Santé Cohort.					
34718693	6	46	theme	24-h	1066:1069	arg1	records					1079:1085	repeated 24-h dietary records	1057:1085	repeated 24-h dietary records linked to a detailed food composition table	1057:1129	Baseline dietary intakes were obtained from repeated 24-h dietary records linked to a detailed food composition table.					
34718693	4	47	dep	OBJECTIVES	578:587	arg1	was					603:605	was	603:605	was to investigate the associations between FODMAP intake (total and by type) and cancer risk (overall, breast, prostate, and colorectal) in a large prospective cohort	603:769	OBJECTIVES Our objective was to investigate the associations between FODMAP intake (total and by type) and cancer risk (overall, breast, prostate, and colorectal) in a large prospective cohort.					
34718693	11	48	theme	potential	2111:2119	arg1	mechanisms					2132:2141	the potential underlying mechanisms	2107:2141	the potential underlying mechanisms	2107:2141	Further epidemiologic and experimental studies are needed to confirm these results and provide data on the potential underlying mechanisms.					
34718693	5	49	dep	follow-up	934:942	arg1	age					981:983	mean ± SD age	971:983	mean ± SD age at baseline 42.1 ± 14.5 y	971:1009	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	5	49	dep	follow-up	934:942	arg1	y					955:955	y	955:955	median follow-up time = 7.7 y	927:955	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	5	49	dep	follow-up	934:942	arg1	women					964:968	78.7% women	958:968	78.7% women	958:968	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	9	50	dep	Q5	1647:1648	arg1	P-trend = 0.04					1694:1707	P-trend = 0.04	1694:1707	P-trend = 0.04	1694:1707	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	9	50	dep	Q5	1647:1648	arg1	%					1676:1676	1.10; 95%	1668:1676	1.10; 95% CI: 0.97	1668:1685	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	9	51	dep	n = 272	1733:1739	arg1	Q5					1745:1746	HR Q5	1742:1746	HR Q5 compared with Q1: 1.78	1742:1769	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	9	51	dep	n = 272	1733:1739	arg1	%					1774:1774	95% CI: 1.13-2.79	1772:1788	95% CI: 1.13-2.79; P-trend = 0.02	1772:1804	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	5	52	theme	adult	850:854	arg1	participants					856:867	104,909 adult participants	842:867	104,909 adult participants without cancer at baseline	842:894	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	8	53	theme	increased	1397:1405	arg1	risk					1422:1425	increased overall cancer risk	1397:1425	increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04)	1397:1534	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	10	54	theme	FODMAP	1954:1959	arg1	intake					1961:1966	FODMAP intake	1954:1966	FODMAP intake	1954:1966	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	4	55	theme	cancer	685:690	arg1	risk					692:695	cancer risk	685:695	cancer risk (overall, breast, prostate, and colorectal)	685:739	OBJECTIVES Our objective was to investigate the associations between FODMAP intake (total and by type) and cancer risk (overall, breast, prostate, and colorectal) in a large prospective cohort.					
34718693	5	56	theme	mean ± SD	971:979	arg1	age					981:983	mean ± SD age	971:983	mean ± SD age at baseline 42.1 ± 14.5 y	971:1009	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	5	56	theme	mean ± SD	971:979	arg1	women					964:968	78.7% women	958:968	78.7% women	958:968	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	9	57	dep	cancer	1725:1730	arg1	n = 272					1733:1739	n = 272	1733:1739	n = 272	1733:1739	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	6	58	theme	dietary	1071:1077	arg1	records					1079:1085	repeated 24-h dietary records	1057:1085	repeated 24-h dietary records linked to a detailed food composition table	1057:1129	Baseline dietary intakes were obtained from repeated 24-h dietary records linked to a detailed food composition table.					
34718693	8	59	theme	cancer	1415:1420	arg1	risk					1422:1425	increased overall cancer risk	1397:1425	increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04)	1397:1534	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	7	60	theme	large	1275:1279	arg1	range					1281:1285	a large range	1273:1285	a large range of lifestyle, sociodemographic, and anthropometric variables	1273:1346	Associations between FODMAP intake (expressed in quintiles, Q) and cancer risks were assessed by Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables.					
34718693	10	61	theme	cancer	1984:1989	arg1	development					1991:2001	cancer development	1984:2001	cancer development	1984:2001	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	10	62	theme	significant	1922:1932	arg1	association					1934:1944	a significant association	1920:1944	a significant association between FODMAP intake and the risk of cancer development	1920:2001	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	6	63	attach	linked	1087:1092	arg1	table					1125:1129	a detailed food composition table	1097:1129	a detailed food composition table	1097:1129	Baseline dietary intakes were obtained from repeated 24-h dietary records linked to a detailed food composition table.					
34718693	6	63	attach	linked	1087:1092	arg2	records					1079:1085	repeated 24-h dietary records	1057:1085	repeated 24-h dietary records linked to a detailed food composition table	1057:1129	Baseline dietary intakes were obtained from repeated 24-h dietary records linked to a detailed food composition table.					
34718693	11	64	theme	underlying	2121:2130	arg1	mechanisms					2132:2141	the potential underlying mechanisms	2107:2141	the potential underlying mechanisms	2107:2141	Further epidemiologic and experimental studies are needed to confirm these results and provide data on the potential underlying mechanisms.					
34718693	11	65	theme	epidemiologic	2012:2024	arg1	studies					2043:2049	Further epidemiologic and experimental studies	2004:2049	Further epidemiologic and experimental studies	2004:2049	Further epidemiologic and experimental studies are needed to confirm these results and provide data on the potential underlying mechanisms.					
34718693	6	66	theme	composition	1113:1123	arg1	table					1125:1129	a detailed food composition table	1097:1129	a detailed food composition table	1097:1129	Baseline dietary intakes were obtained from repeated 24-h dietary records linked to a detailed food composition table.					
34718693	5	67	dep	included	901:908	arg1	follow-up					934:942	median follow-up time = 7.7	927:953	median follow-up time = 7.7 y	927:955	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	1	68	theme	Fermentable	153:163	arg1	FODMAPs					228:234	FODMAPs	228:234	FODMAPs	228:234	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) have been shown to be involved in gastrointestinal disorders.					
34718693	1	68	theme	Fermentable	153:163	arg1	oligosaccharides					165:180	BACKGROUND Fermentable oligosaccharides	142:180	BACKGROUND Fermentable oligosaccharides	142:180	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) have been shown to be involved in gastrointestinal disorders.					
34718693	2	69	with	view	302:305	arg1	microbiota					378:387	the gut microbiota	370:387	the gut microbiota	370:387	In view of their proinflammatory potential and their interactions with the gut microbiota, their contribution to the etiology of other chronic diseases such as cancer has been postulated.					
34718693	8	70	theme	incident	1437:1444	arg1	HR					1453:1454	HR	1453:1454	HR for sex-specific Q5 compared with Q1	1453:1491	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	8	70	theme	incident	1437:1444	arg1	cases					1446:1450	n = 3374 incident cases	1428:1450	n = 3374 incident cases	1428:1450	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	10	71	theme	CONCLUSIONS	1808:1818	arg1	Results					1820:1826	CONCLUSIONS Results	1808:1826	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort	1808:1913	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	0	72	theme	Cancer	88:93	arg1	Risk					95:98	Cancer Risk	88:98	Cancer Risk	88:98	Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols (FODMAPs) and Cancer Risk in the Prospective NutriNet-Santé Cohort.					
34718693	9	73	theme	HR	1644:1645	arg1	cancer					1636:1641	overall cancer	1628:1641	overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04)	1628:1708	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	9	73	theme	HR	1644:1645	arg1	Q5					1647:1648	HR Q5	1644:1648	HR Q5 compared with Q1	1644:1665	In particular, oligosaccharides were associated with cancer risk: a trend was observed for overall cancer (HR Q5 compared with Q1: 1.10; 95% CI: 0.97, 1.25; P-trend = 0.04) and colorectal cancer (n = 272, HR Q5 compared with Q1: 1.78; 95% CI: 1.13-2.79; P-trend = 0.02).					
34718693	5	74	from	baseline	988:995	arg1	age					981:983	mean ± SD age	971:983	mean ± SD age at baseline 42.1 ± 14.5 y	971:1009	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	5	74	from	baseline	988:995	arg1	women					964:968	78.7% women	958:968	78.7% women	958:968	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	7	75	theme	hazard	1246:1251	arg1	models					1253:1258	Cox proportional hazard models	1229:1258	Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables	1229:1346	Associations between FODMAP intake (expressed in quintiles, Q) and cancer risks were assessed by Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables.					
34718693	11	76	theme	experimental	2030:2041	arg1	studies					2043:2049	Further epidemiologic and experimental studies	2004:2049	Further epidemiologic and experimental studies	2004:2049	Further epidemiologic and experimental studies are needed to confirm these results and provide data on the potential underlying mechanisms.					
34718693	7	77	theme	Cox	1229:1231	arg1	models					1253:1258	Cox proportional hazard models	1229:1258	Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables	1229:1346	Associations between FODMAP intake (expressed in quintiles, Q) and cancer risks were assessed by Cox proportional hazard models adjusted for a large range of lifestyle, sociodemographic, and anthropometric variables.					
34718693	2	78	theme	diseases	442:449	arg1	etiology					416:423	the etiology	412:423	the etiology of other chronic diseases such as cancer	412:464	In view of their proinflammatory potential and their interactions with the gut microbiota, their contribution to the etiology of other chronic diseases such as cancer has been postulated.					
34718693	10	79	from	study	1861:1865	arg1	adults					1877:1882	French adults	1870:1882	French adults from the NutriNet-Santé cohort	1870:1913	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	10	79	from	study	1861:1865	arg1	Results					1820:1826	CONCLUSIONS Results	1808:1826	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort	1808:1913	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	1	80	theme	gastrointestinal	271:286	arg1	disorders					288:296	gastrointestinal disorders	271:296	gastrointestinal disorders	271:296	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) have been shown to be involved in gastrointestinal disorders.					
34718693	4	81	dep	risk	692:695	arg1	prostate					715:722	prostate	715:722	prostate	715:722	OBJECTIVES Our objective was to investigate the associations between FODMAP intake (total and by type) and cancer risk (overall, breast, prostate, and colorectal) in a large prospective cohort.					
34718693	4	81	dep	risk	692:695	arg1	colorectal					729:738	colorectal	729:738	colorectal	729:738	OBJECTIVES Our objective was to investigate the associations between FODMAP intake (total and by type) and cancer risk (overall, breast, prostate, and colorectal) in a large prospective cohort.					
34718693	4	81	dep	risk	692:695	arg1	breast					707:712	breast	707:712	breast	707:712	OBJECTIVES Our objective was to investigate the associations between FODMAP intake (total and by type) and cancer risk (overall, breast, prostate, and colorectal) in a large prospective cohort.					
34718693	2	82	theme	other	428:432	arg1	diseases					442:449	other chronic diseases	428:449	other chronic diseases such as cancer	428:464	In view of their proinflammatory potential and their interactions with the gut microbiota, their contribution to the etiology of other chronic diseases such as cancer has been postulated.					
34718693	2	82	theme	other	428:432	arg1	cancer					459:464	cancer	459:464	cancer	459:464	In view of their proinflammatory potential and their interactions with the gut microbiota, their contribution to the etiology of other chronic diseases such as cancer has been postulated.					
34718693	5	83	theme	%	962:962	arg1	age					981:983	mean ± SD age	971:983	mean ± SD age at baseline 42.1 ± 14.5 y	971:1009	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	5	83	theme	%	962:962	arg1	women					964:968	78.7% women	958:968	78.7% women	958:968	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	6	84	theme	repeated	1057:1064	arg1	records					1079:1085	repeated 24-h dietary records	1057:1085	repeated 24-h dietary records linked to a detailed food composition table	1057:1129	Baseline dietary intakes were obtained from repeated 24-h dietary records linked to a detailed food composition table.					
34718693	4	85	theme	large	746:750	arg1	cohort					764:769	a large prospective cohort	744:769	a large prospective cohort	744:769	OBJECTIVES Our objective was to investigate the associations between FODMAP intake (total and by type) and cancer risk (overall, breast, prostate, and colorectal) in a large prospective cohort.					
34718693	2	86	with	interactions	352:363	arg1	microbiota					378:387	the gut microbiota	370:387	the gut microbiota	370:387	In view of their proinflammatory potential and their interactions with the gut microbiota, their contribution to the etiology of other chronic diseases such as cancer has been postulated.					
34718693	5	87	theme	median	927:932	arg1	follow-up					934:942	median follow-up time = 7.7	927:953	median follow-up time = 7.7 y	927:955	METHODS The study was based on the NutriNet-Santé cohort (2009-2020); 104,909 adult participants without cancer at baseline were included in our analyses (median follow-up time = 7.7 y, 78.7% women, mean ± SD age at baseline 42.1 ± 14.5 y).					
34718693	10	88	theme	large	1838:1842	arg1	study					1861:1865	this large population-based study	1833:1865	this large population-based study on French adults from the NutriNet-Santé cohort	1833:1913	CONCLUSIONS Results from this large population-based study on French adults from the NutriNet-Santé cohort show a significant association between FODMAP intake and the risk of cancer development.					
34718693	2	89	theme	proinflammatory	316:330	arg1	potential					332:340	their proinflammatory potential	310:340	their proinflammatory potential	310:340	In view of their proinflammatory potential and their interactions with the gut microbiota, their contribution to the etiology of other chronic diseases such as cancer has been postulated.					
34718693	0	90	dep	Oligosaccharides	12:27	arg1	Cohort					134:139	the Prospective NutriNet-Santé Cohort	103:139	the Prospective NutriNet-Santé Cohort	103:139	Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols (FODMAPs) and Cancer Risk in the Prospective NutriNet-Santé Cohort.					
34718693	8	91	theme	RESULTS	1349:1355	arg1	intake					1370:1375	RESULTS Total FODMAP intake	1349:1375	RESULTS Total FODMAP intake	1349:1375	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	8	92	dep	cases	1446:1450	arg1	P-trend = 0.04					1520:1533	P-trend = 0.04	1520:1533	P-trend = 0.04	1520:1533	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	8	92	dep	cases	1446:1450	arg1	1.21					1494:1497	1.21	1494:1497	1.21	1494:1497	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
34718693	8	92	dep	cases	1446:1450	arg1	%					1502:1502	95% CI: 1.02	1500:1511	95% CI: 1.02	1500:1511	RESULTS Total FODMAP intake was associated with increased overall cancer risk (n = 3374 incident cases, HR for sex-specific Q5 compared with Q1: 1.21; 95% CI: 1.02, 1.44; P-trend = 0.04).					
37220126	2	0	theme	biochemical	373:383	arg1	standard					505:512	standard	505:512	standard	505:512	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	2	0	theme	biochemical	373:383	arg1	related					440:446	related	440:446	related	440:446	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	2	0	theme	biochemical	373:383	arg1	properties					403:412	the biochemical and biomechanical properties	369:412	the biochemical and biomechanical properties	369:412	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	1	1	theme	articular	275:283	arg1	damage					295:300	extensive articular cartilage damage	265:300	extensive articular cartilage damage	265:300	Osteochondral allograft (OCA) is an important surgical procedure used to repair extensive articular cartilage damage.					
37220126	10	2	theme	OCAs	1824:1827	arg1	success					1813:1819	the functional success	1798:1819	the functional success of OCAs	1798:1827	In conclusion, we evaluated the biochemical composition of OCAs before and after surgery using μCT analysis and demonstrated that the GAG content of the graft decreased, it also decreased during implantation; this resulted in decreased chondrocyte viability after transplantation and ultimately affected the functional success of OCAs.					
37220126	9	3	theme	4h	1348:1349	arg1	groups					1358:1363	the 4h and 8h groups	1344:1363	groups	1358:1363	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	9	4	theme	8h	1355:1356	arg1	groups					1358:1363	the 4h and 8h groups	1344:1363	groups	1358:1363	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	8	5	theme	surgery	1174:1180	arg1	effects					1182:1188	transplant surgery effects	1163:1188	transplant surgery effects	1163:1188	In this study, transplant surgery effects were assessed using micro-computed tomography (μCT) and histological analysis.					
37220126	0	6	theme	allografts	126:135	arg1	properties					98:107	the functional properties	83:107	the functional properties of osteochondral allografts	83:135	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	7	7	theme	treated	1090:1096	arg1	OCAs					1098:1101	The treated OCAs	1086:1101	The treated OCAs of each group	1086:1115	The treated OCAs of each group were used for transplantation.					
37220126	1	8	theme	surgical	231:238	arg1	procedure					240:248	an important surgical procedure	218:248	an important surgical procedure used to repair extensive articular cartilage damage	218:300	Osteochondral allograft (OCA) is an important surgical procedure used to repair extensive articular cartilage damage.					
37220126	1	8	theme	surgical	231:238	arg1	allograft					199:207	Osteochondral allograft	185:207	Osteochondral allograft (OCA)	185:213	Osteochondral allograft (OCA) is an important surgical procedure used to repair extensive articular cartilage damage.					
37220126	0	9	theme	non-destructive	159:173	arg1	analysis					175:182	micro-CT non-destructive analysis	150:182	micro-CT non-destructive analysis	150:182	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	3	10	theme	research	589:596	arg1	lack					570:573	a lack	568:573	a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation	568:703	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	3	11	theme	content	619:625	arg1	effect					605:610	the effect	601:610	the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation	601:703	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	0	12	theme	donor	64:68	arg1	cartilage					70:78	donor cartilage	64:78	donor cartilage	64:78	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	6	13	theme	chondroitinase	975:988	arg1	times					966:970	the different action times	945:970	the different action times of chondroitinase	945:988	Due to the different action times of chondroitinase, they were divided into 4 experimental groups (including control group, 2h, 4h, and 8h groups).					
37220126	9	14	dep	modulus	1446:1452	arg1	as					1422:1423	as	1422:1423	as	1422:1423	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	9	14	dep	modulus	1446:1452	arg1	were					1425:1428	were	1425:1428	were	1425:1428	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	1	15	theme	Osteochondral	185:197	arg1	OCA					210:212	OCA	210:212	OCA	210:212	Osteochondral allograft (OCA) is an important surgical procedure used to repair extensive articular cartilage damage.					
37220126	1	15	theme	Osteochondral	185:197	arg1	allograft					199:207	Osteochondral allograft	185:207	Osteochondral allograft (OCA)	185:213	Osteochondral allograft (OCA) is an important surgical procedure used to repair extensive articular cartilage damage.					
37220126	1	15	theme	Osteochondral	185:197	arg1	procedure					240:248	an important surgical procedure	218:248	an important surgical procedure used to repair extensive articular cartilage damage	218:300	Osteochondral allograft (OCA) is an important surgical procedure used to repair extensive articular cartilage damage.					
37220126	3	16	theme	matrix	639:644	arg1	content					619:625	the content	615:625	the content of cellular matrix in OCA cartilage tissue	615:668	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	2	17	theme	OCA	545:547	arg1	evaluation					531:540	preoperative evaluation	518:540	preoperative evaluation of OCA	518:547	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	0	18	from	Effects	0:6	arg1	properties					98:107	the functional properties	83:107	the functional properties of osteochondral allografts	83:135	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	0	18	from	Effects	0:6	arg1	matrix					54:59	extracellular matrix	40:59	extracellular matrix of donor cartilage	40:78	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	4	19	theme	GAG	754:756	arg1	contents					758:765	different GAG contents	744:765	different GAG contents	744:765	Therefore, we evaluated the effect of different GAG contents on the success of OCA transplantation in a rabbit animal model.					
37220126	0	20	theme	functional	87:96	arg1	properties					98:107	the functional properties	83:107	the functional properties of osteochondral allografts	83:135	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	6	21	theme	different	949:957	arg1	times					966:970	the different action times	945:970	the different action times of chondroitinase	945:988	Due to the different action times of chondroitinase, they were divided into 4 experimental groups (including control group, 2h, 4h, and 8h groups).					
37220126	0	22	from	matrix	54:59	arg1	Effects					0:6	Effects	0:6	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts	0:135	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	9	23	theme	compressive	1434:1444	arg1	modulus					1446:1452	the compressive modulus	1430:1452	the compressive modulus	1430:1452	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	3	24	from	content	619:625	arg1	tissue					663:668	OCA cartilage tissue	649:668	OCA cartilage tissue	649:668	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	2	25	theme	OCA	417:419	arg1	standard					505:512	standard	505:512	standard	505:512	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	2	25	theme	OCA	417:419	arg1	related					440:446	related	440:446	related	440:446	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	2	25	theme	OCA	417:419	arg1	properties					403:412	the biochemical and biomechanical properties	369:412	the biochemical and biomechanical properties	369:412	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	7	26	used	used	1122:1125	arg2	OCAs					1098:1101	The treated OCAs	1086:1101	The treated OCAs of each group	1086:1115	The treated OCAs of each group were used for transplantation.					
37220126	2	27	theme	clinical	455:462	arg1	success					464:470	the clinical success	451:470	the clinical success of the operation	451:487	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	3	28	from	research	589:596	arg1	effect					605:610	the effect	601:610	the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation	601:703	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	10	29	theme	OCAs	1553:1556	arg1	composition					1538:1548	the biochemical composition	1522:1548	the biochemical composition of OCAs before and after surgery	1522:1581	In conclusion, we evaluated the biochemical composition of OCAs before and after surgery using μCT analysis and demonstrated that the GAG content of the graft decreased, it also decreased during implantation; this resulted in decreased chondrocyte viability after transplantation and ultimately affected the functional success of OCAs.					
37220126	0	30	theme	glycosaminoglycan	11:27	arg1	content					29:35	glycosaminoglycan content	11:35	glycosaminoglycan content in extracellular matrix of donor cartilage	11:78	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	2	31	theme	operation	479:487	arg1	success					464:470	the clinical success	451:470	the clinical success of the operation	451:487	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	3	32	theme	cellular	630:637	arg1	matrix					639:644	cellular matrix	630:644	cellular matrix	630:644	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	4	33	theme	rabbit	810:815	arg1	model					824:828	a rabbit animal model	808:828	a rabbit animal model	808:828	Therefore, we evaluated the effect of different GAG contents on the success of OCA transplantation in a rabbit animal model.					
37220126	4	34	theme	transplantation	789:803	arg1	success					774:780	the success	770:780	the success of OCA transplantation	770:803	Therefore, we evaluated the effect of different GAG contents on the success of OCA transplantation in a rabbit animal model.					
37220126	9	35	from	groups	1358:1363	arg1	poorer					1334:1339	poorer	1334:1339	poorer	1334:1339	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	10	36	theme	chondrocyte	1730:1740	arg1	viability					1742:1750	decreased chondrocyte viability	1720:1750	decreased chondrocyte viability	1720:1750	In conclusion, we evaluated the biochemical composition of OCAs before and after surgery using μCT analysis and demonstrated that the GAG content of the graft decreased, it also decreased during implantation; this resulted in decreased chondrocyte viability after transplantation and ultimately affected the functional success of OCAs.					
37220126	4	37	theme	OCA	785:787	arg1	transplantation					789:803	OCA transplantation	785:803	OCA transplantation	785:803	Therefore, we evaluated the effect of different GAG contents on the success of OCA transplantation in a rabbit animal model.					
37220126	3	38	from	tissue	663:668	arg1	effect					605:610	the effect	601:610	the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation	601:703	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	3	39	theme	transplantation	689:703	arg1	efficacy					677:684	the efficacy	673:684	the efficacy of transplantation	673:703	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	10	40	theme	μCT	1589:1591	arg1	analysis					1593:1600	μCT analysis	1589:1600	μCT analysis	1589:1600	In conclusion, we evaluated the biochemical composition of OCAs before and after surgery using μCT analysis and demonstrated that the GAG content of the graft decreased, it also decreased during implantation; this resulted in decreased chondrocyte viability after transplantation and ultimately affected the functional success of OCAs.					
37220126	1	41	theme	extensive	265:273	arg1	damage					295:300	extensive articular cartilage damage	265:300	extensive articular cartilage damage	265:300	Osteochondral allograft (OCA) is an important surgical procedure used to repair extensive articular cartilage damage.					
37220126	10	42	theme	GAG	1628:1630	arg1	content					1632:1638	the GAG content	1624:1638	the GAG content of the graft	1624:1651	In conclusion, we evaluated the biochemical composition of OCAs before and after surgery using μCT analysis and demonstrated that the GAG content of the graft decreased, it also decreased during implantation; this resulted in decreased chondrocyte viability after transplantation and ultimately affected the functional success of OCAs.					
37220126	1	43	theme	cartilage	285:293	arg1	damage					295:300	extensive articular cartilage damage	265:300	extensive articular cartilage damage	265:300	Osteochondral allograft (OCA) is an important surgical procedure used to repair extensive articular cartilage damage.					
37220126	9	44	from	weeks	1407:1411	arg1	group					1389:1393	the control group	1377:1393	the control group at 4 and 12 weeks	1377:1411	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	5	45	theme	rabbit	836:841	arg1	OCA					843:845	Each rabbit OCA	831:845	Each rabbit OCA	831:845	Each rabbit OCA was treated with chondroitinase to regulate glycosaminoglycan (GAG) content in the tissue.					
37220126	4	46	theme	animal	817:822	arg1	model					824:828	a rabbit animal model	808:828	a rabbit animal model	808:828	Therefore, we evaluated the effect of different GAG contents on the success of OCA transplantation in a rabbit animal model.					
37220126	10	47	theme	functional	1802:1811	arg1	success					1813:1819	the functional success	1798:1819	the functional success of OCAs	1798:1827	In conclusion, we evaluated the biochemical composition of OCAs before and after surgery using μCT analysis and demonstrated that the GAG content of the graft decreased, it also decreased during implantation; this resulted in decreased chondrocyte viability after transplantation and ultimately affected the functional success of OCAs.					
37220126	10	48	theme	graft	1647:1651	arg1	content					1632:1638	the GAG content	1624:1638	the GAG content of the graft	1624:1651	In conclusion, we evaluated the biochemical composition of OCAs before and after surgery using μCT analysis and demonstrated that the GAG content of the graft decreased, it also decreased during implantation; this resulted in decreased chondrocyte viability after transplantation and ultimately affected the functional success of OCAs.					
37220126	8	49	theme	transplant	1163:1172	arg1	effects					1182:1188	transplant surgery effects	1163:1188	transplant surgery effects	1163:1188	In this study, transplant surgery effects were assessed using micro-computed tomography (μCT) and histological analysis.					
37220126	2	50	theme	chondrocyte	320:330	arg1	viability					332:340	chondrocyte viability	320:340	chondrocyte viability	320:340	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	10	51	dep	decreased	1653:1661	arg1	decreased					1672:1680	decreased	1672:1680	decreased during implantation	1672:1700	In conclusion, we evaluated the biochemical composition of OCAs before and after surgery using μCT analysis and demonstrated that the GAG content of the graft decreased, it also decreased during implantation; this resulted in decreased chondrocyte viability after transplantation and ultimately affected the functional success of OCAs.					
37220126	0	52	theme	micro-CT	150:157	arg1	analysis					175:182	micro-CT non-destructive analysis	150:182	micro-CT non-destructive analysis	150:182	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	9	53	theme	control	1381:1387	arg1	group					1389:1393	the control group	1377:1393	the control group at 4 and 12 weeks	1377:1411	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	3	54	theme	systematic	578:587	arg1	research					589:596	systematic research	578:596	systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation	578:703	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	5	55	theme	glycosaminoglycan	891:907	arg1	content					915:921	glycosaminoglycan (GAG) content	891:921	glycosaminoglycan (GAG) content in the tissue	891:935	Each rabbit OCA was treated with chondroitinase to regulate glycosaminoglycan (GAG) content in the tissue.					
37220126	8	56	theme	micro-computed	1210:1223	arg1	μCT					1237:1239	μCT	1237:1239	μCT	1237:1239	In this study, transplant surgery effects were assessed using micro-computed tomography (μCT) and histological analysis.					
37220126	8	56	theme	micro-computed	1210:1223	arg1	tomography					1225:1234	micro-computed tomography	1210:1234	micro-computed tomography (μCT)	1210:1240	In this study, transplant surgery effects were assessed using micro-computed tomography (μCT) and histological analysis.					
37220126	9	57	dep	poorer	1334:1339	arg1	compared					1365:1372	compared	1365:1372	compared to the control group at 4 and 12 weeks in vivo	1365:1419	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	3	58	from	lack	570:573	arg1	effect					605:610	the effect	601:610	the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation	601:703	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	0	59	theme	cartilage	70:78	arg1	matrix					54:59	extracellular matrix	40:59	extracellular matrix of donor cartilage	40:78	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	0	60	from	content	29:35	arg1	matrix					54:59	extracellular matrix	40:59	extracellular matrix of donor cartilage	40:78	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	8	61	theme	histological	1246:1257	arg1	analysis					1259:1266	histological analysis	1246:1266	histological analysis	1246:1266	In this study, transplant surgery effects were assessed using micro-computed tomography (μCT) and histological analysis.					
37220126	6	62	theme	action	959:964	arg1	times					966:970	the different action times	945:970	the different action times of chondroitinase	945:988	Due to the different action times of chondroitinase, they were divided into 4 experimental groups (including control group, 2h, 4h, and 8h groups).					
37220126	4	63	theme	different	744:752	arg1	contents					758:765	different GAG contents	744:765	different GAG contents	744:765	Therefore, we evaluated the effect of different GAG contents on the success of OCA transplantation in a rabbit animal model.					
37220126	3	64	from	effect	605:610	arg1	tissue					663:668	OCA cartilage tissue	649:668	OCA cartilage tissue	649:668	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	3	64	from	effect	605:610	arg1	lack					570:573	a lack	568:573	a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation	568:703	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	3	64	from	effect	605:610	arg1	efficacy					677:684	the efficacy	673:684	the efficacy of transplantation	673:703	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	0	65	theme	osteochondral	112:124	arg1	allografts					126:135	osteochondral allografts	112:135	osteochondral allografts	112:135	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	6	66	theme	control	1047:1053	arg1	group					1055:1059	control group	1047:1059	control group	1047:1059	Due to the different action times of chondroitinase, they were divided into 4 experimental groups (including control group, 2h, 4h, and 8h groups).					
37220126	2	67	theme	preoperative	518:529	arg1	evaluation					531:540	preoperative evaluation	518:540	preoperative evaluation of OCA	518:547	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	4	68	theme	contents	758:765	arg1	effect					734:739	the effect	730:739	the effect of different GAG contents on the success of OCA transplantation	730:803	Therefore, we evaluated the effect of different GAG contents on the success of OCA transplantation in a rabbit animal model.					
37220126	7	69	theme	group	1111:1115	arg1	OCAs					1098:1101	The treated OCAs	1086:1101	The treated OCAs of each group	1086:1115	The treated OCAs of each group were used for transplantation.					
37220126	4	70	from	effect	734:739	arg1	success					774:780	the success	770:780	the success of OCA transplantation	770:803	Therefore, we evaluated the effect of different GAG contents on the success of OCA transplantation in a rabbit animal model.					
37220126	3	71	theme	cartilage	653:661	arg1	tissue					663:668	OCA cartilage tissue	649:668	OCA cartilage tissue	649:668	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	0	72	theme	content	29:35	arg1	Effects					0:6	Effects	0:6	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts	0:135	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	9	73	theme	GAG	1455:1457	arg1	content					1459:1465	GAG content	1455:1465	GAG content	1455:1465	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	6	74	theme	experimental	1016:1027	arg1	2h					1062:1063	2h	1062:1063	2h	1062:1063	Due to the different action times of chondroitinase, they were divided into 4 experimental groups (including control group, 2h, 4h, and 8h groups).					
37220126	6	74	theme	experimental	1016:1027	arg1	group					1055:1059	control group	1047:1059	control group	1047:1059	Due to the different action times of chondroitinase, they were divided into 4 experimental groups (including control group, 2h, 4h, and 8h groups).					
37220126	6	74	theme	experimental	1016:1027	arg1	4h					1066:1067	4h	1066:1067	4h	1066:1067	Due to the different action times of chondroitinase, they were divided into 4 experimental groups (including control group, 2h, 4h, and 8h groups).					
37220126	6	74	theme	experimental	1016:1027	arg1	groups					1029:1034	4 experimental groups	1014:1034	4 experimental groups (including control group, 2h, 4h, and 8h groups)	1014:1083	Due to the different action times of chondroitinase, they were divided into 4 experimental groups (including control group, 2h, 4h, and 8h groups).					
37220126	6	74	theme	experimental	1016:1027	arg1	8h					1074:1075	8h	1074:1075	8h	1074:1075	Due to the different action times of chondroitinase, they were divided into 4 experimental groups (including control group, 2h, 4h, and 8h groups).					
37220126	10	75	theme	decreased	1720:1728	arg1	viability					1742:1750	decreased chondrocyte viability	1720:1750	decreased chondrocyte viability	1720:1750	In conclusion, we evaluated the biochemical composition of OCAs before and after surgery using μCT analysis and demonstrated that the GAG content of the graft decreased, it also decreased during implantation; this resulted in decreased chondrocyte viability after transplantation and ultimately affected the functional success of OCAs.					
37220126	0	76	theme	extracellular	40:52	arg1	matrix					54:59	extracellular matrix	40:59	extracellular matrix of donor cartilage	40:78	Effects of glycosaminoglycan content in extracellular matrix of donor cartilage on the functional properties of osteochondral allografts evaluated by micro-CT non-destructive analysis.					
37220126	9	77	from	poorer	1334:1339	arg1	groups					1358:1363	the 4h and 8h groups	1344:1363	groups	1358:1363	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	1	78	theme	important	221:229	arg1	procedure					240:248	an important surgical procedure	218:248	an important surgical procedure used to repair extensive articular cartilage damage	218:300	Osteochondral allograft (OCA) is an important surgical procedure used to repair extensive articular cartilage damage.					
37220126	1	78	theme	important	221:229	arg1	allograft					199:207	Osteochondral allograft	185:207	Osteochondral allograft (OCA)	185:213	Osteochondral allograft (OCA) is an important surgical procedure used to repair extensive articular cartilage damage.					
37220126	6	79	dep	group	1055:1059	arg1	groups					1077:1082	groups	1077:1082	groups	1077:1082	Due to the different action times of chondroitinase, they were divided into 4 experimental groups (including control group, 2h, 4h, and 8h groups).					
37220126	10	80	theme	biochemical	1526:1536	arg1	composition					1538:1548	the biochemical composition	1522:1548	the biochemical composition of OCAs before and after surgery	1522:1581	In conclusion, we evaluated the biochemical composition of OCAs before and after surgery using μCT analysis and demonstrated that the GAG content of the graft decreased, it also decreased during implantation; this resulted in decreased chondrocyte viability after transplantation and ultimately affected the functional success of OCAs.					
37220126	9	81	theme	tissue	1293:1298	arg1	integration					1300:1310	tissue integration	1293:1310	tissue integration at the graft site	1293:1328	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	9	82	from	site	1325:1328	arg1	integration					1300:1310	tissue integration	1293:1310	tissue integration at the graft site	1293:1328	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	9	83	theme	cell	1472:1475	arg1	density					1477:1483	cell density	1472:1483	cell density reduced	1472:1491	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	3	84	theme	OCA	649:651	arg1	tissue					663:668	OCA cartilage tissue	649:668	OCA cartilage tissue	649:668	However, there is a lack of systematic research on the effect of the content of cellular matrix in OCA cartilage tissue on the efficacy of transplantation.					
37220126	5	85	from	content	915:921	arg1	tissue					930:935	the tissue	926:935	the tissue	926:935	Each rabbit OCA was treated with chondroitinase to regulate glycosaminoglycan (GAG) content in the tissue.					
37220126	9	86	theme	graft	1319:1323	arg1	site					1325:1328	the graft site	1315:1328	the graft site	1315:1328	Our results showed that tissue integration at the graft site was poorer in the 4h and 8h groups compared to the control group at 4 and 12 weeks in vivo, as were the compressive modulus, GAG content, and cell density reduced.					
37220126	2	87	theme	biomechanical	389:401	arg1	standard					505:512	standard	505:512	standard	505:512	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	2	87	theme	biomechanical	389:401	arg1	related					440:446	related	440:446	related	440:446	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
37220126	2	87	theme	biomechanical	389:401	arg1	properties					403:412	the biochemical and biomechanical properties	369:412	the biochemical and biomechanical properties	369:412	It is known that chondrocyte viability is crucial for maintaining the biochemical and biomechanical properties of OCA, which is directly related to the clinical success of the operation and is the only standard for preoperative evaluation of OCA.					
36876784	4	0	theme	granule	506:512	arg1	extraction					514:523	high-throughput starch granule extraction	483:523	high-throughput starch granule extraction	483:523	Here, we present two approaches for high-throughput starch granule extraction and size determination using flow cytometry and automated, high-throughput light microscopy.					
36876784	4	1	theme	size	529:532	arg1	determination					534:546	size determination	529:546	size determination	529:546	Here, we present two approaches for high-throughput starch granule extraction and size determination using flow cytometry and automated, high-throughput light microscopy.					
36876784	5	2	theme	different	682:690	arg1	species					692:698	different species	682:698	different species	682:698	We evaluated the practicality of both methods using starch from different species and tissues and demonstrated their effectiveness by screening for induced variation in starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules.					
36876784	4	3	theme	light	600:604	arg1	microscopy					606:615	automated, high-throughput light microscopy	573:615	automated, high-throughput light microscopy	573:615	Here, we present two approaches for high-throughput starch granule extraction and size determination using flow cytometry and automated, high-throughput light microscopy.					
36876784	5	4	theme	induced	766:772	arg1	variation					774:782	induced variation	766:782	induced variation in starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules	766:923	We evaluated the practicality of both methods using starch from different species and tissues and demonstrated their effectiveness by screening for induced variation in starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules.					
36876784	6	5	theme	Arabidopsis	938:948	arg1	lines					950:954	Arabidopsis lines	938:954	Arabidopsis lines altered in starch biosynthesis	938:985	Analysis of Arabidopsis lines altered in starch biosynthesis further demonstrates the applicability of these approaches.					
36876784	6	6	theme	starch	967:972	arg1	biosynthesis					974:985	starch biosynthesis	967:985	starch biosynthesis	967:985	Analysis of Arabidopsis lines altered in starch biosynthesis further demonstrates the applicability of these approaches.					
36876784	0	7	theme	flow	92:95	arg1	cytometry					97:105	flow cytometry	92:105	flow cytometry	92:105	Detecting variation in starch granule size and morphology by high-throughput microscopy and flow cytometry.					
36876784	6	8	theme	approaches	1035:1044	arg1	applicability					1012:1024	the applicability	1008:1024	the applicability of these approaches	1008:1044	Analysis of Arabidopsis lines altered in starch biosynthesis further demonstrates the applicability of these approaches.					
36876784	3	9	from	differences	389:399	arg1	size					419:422	starch granule size	404:422	starch granule size	404:422	However, screening methods to identify differences in starch granule size and shape are lacking.					
36876784	3	9	from	differences	389:399	arg1	shape					428:432	shape	428:432	shape	428:432	However, screening methods to identify differences in starch granule size and shape are lacking.					
36876784	5	10	theme	large	888:892	arg1	A-granules					894:903	large A-granules	888:903	large A-granules	888:903	We evaluated the practicality of both methods using starch from different species and tissues and demonstrated their effectiveness by screening for induced variation in starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules.					
36876784	3	11	theme	screening	359:367	arg1	methods					369:375	screening methods	359:375	screening methods to identify differences in starch granule size and shape	359:432	However, screening methods to identify differences in starch granule size and shape are lacking.					
36876784	4	12	theme	starch	499:504	arg1	extraction					514:523	high-throughput starch granule extraction	483:523	high-throughput starch granule extraction	483:523	Here, we present two approaches for high-throughput starch granule extraction and size determination using flow cytometry and automated, high-throughput light microscopy.					
36876784	5	13	theme	methods	656:662	arg1	practicality					635:646	the practicality	631:646	the practicality of both methods using starch	631:675	We evaluated the practicality of both methods using starch from different species and tissues and demonstrated their effectiveness by screening for induced variation in starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules.					
36876784	5	14	theme	A-granules	894:903	arg1	ratio					879:883	the ratio	875:883	the ratio of large A-granules to small B-granules	875:923	We evaluated the practicality of both methods using starch from different species and tissues and demonstrated their effectiveness by screening for induced variation in starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules.					
36876784	4	15	theme	high-throughput	483:497	arg1	extraction					514:523	high-throughput starch granule extraction	483:523	high-throughput starch granule extraction	483:523	Here, we present two approaches for high-throughput starch granule extraction and size determination using flow cytometry and automated, high-throughput light microscopy.					
36876784	5	16	from	changes	864:870	arg1	ratio					879:883	the ratio	875:883	the ratio of large A-granules to small B-granules	875:923	We evaluated the practicality of both methods using starch from different species and tissues and demonstrated their effectiveness by screening for induced variation in starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules.					
36876784	3	17	theme	starch	404:409	arg1	size					419:422	starch granule size	404:422	starch granule size	404:422	However, screening methods to identify differences in starch granule size and shape are lacking.					
36876784	4	18	theme	automated	573:581	arg1	microscopy					606:615	automated, high-throughput light microscopy	573:615	automated, high-throughput light microscopy	573:615	Here, we present two approaches for high-throughput starch granule extraction and size determination using flow cytometry and automated, high-throughput light microscopy.					
36876784	5	19	dep	lines	828:832	arg1	yielding					835:842	yielding	835:842	yielding four with heritable changes in the ratio of large A-granules to small B-granules	835:923	We evaluated the practicality of both methods using starch from different species and tissues and demonstrated their effectiveness by screening for induced variation in starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules.					
36876784	2	20	theme	physicochemical	312:326	arg1	properties					328:337	the physicochemical properties	308:337	the physicochemical properties of starch	308:347	These traits, together with polymer composition and structure, determine the physicochemical properties of starch.					
36876784	5	21	theme	small	908:912	arg1	B-granules					914:923	small B-granules	908:923	small B-granules	908:923	We evaluated the practicality of both methods using starch from different species and tissues and demonstrated their effectiveness by screening for induced variation in starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules.					
36876784	0	22	theme	starch	23:28	arg1	size					38:41	starch granule size	23:41	starch granule size	23:41	Detecting variation in starch granule size and morphology by high-throughput microscopy and flow cytometry.					
36876784	7	23	theme	trait-controlling	1132:1148	arg1	genes					1150:1154	trait-controlling genes	1132:1154	trait-controlling genes for developing crops with desired properties	1132:1199	Identifying variation in starch granule size and shape will enable identification of trait-controlling genes for developing crops with desired properties, and could help optimise starch processing.					
36876784	7	24	theme	genes	1150:1154	arg1	identification					1114:1127	identification	1114:1127	identification of trait-controlling genes for developing crops with desired properties	1114:1199	Identifying variation in starch granule size and shape will enable identification of trait-controlling genes for developing crops with desired properties, and could help optimise starch processing.					
36876784	5	25	theme	barley	821:826	arg1	lines					828:832	over 10,000 barley lines	809:832	over 10,000 barley lines	809:832	We evaluated the practicality of both methods using starch from different species and tissues and demonstrated their effectiveness by screening for induced variation in starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules.					
36876784	7	26	from	variation	1059:1067	arg1	shape					1096:1100	shape	1096:1100	shape	1096:1100	Identifying variation in starch granule size and shape will enable identification of trait-controlling genes for developing crops with desired properties, and could help optimise starch processing.					
36876784	7	26	from	variation	1059:1067	arg1	size					1087:1090	starch granule size	1072:1090	starch granule size	1072:1090	Identifying variation in starch granule size and shape will enable identification of trait-controlling genes for developing crops with desired properties, and could help optimise starch processing.					
36876784	0	27	theme	granule	30:36	arg1	size					38:41	starch granule size	23:41	starch granule size	23:41	Detecting variation in starch granule size and morphology by high-throughput microscopy and flow cytometry.					
36876784	7	28	theme	starch	1226:1231	arg1	processing					1233:1242	starch processing	1226:1242	starch processing	1226:1242	Identifying variation in starch granule size and shape will enable identification of trait-controlling genes for developing crops with desired properties, and could help optimise starch processing.					
36876784	5	29	theme	heritable	854:862	arg1	changes					864:870	heritable changes	854:870	heritable changes in the ratio of large A-granules to small B-granules	854:923	We evaluated the practicality of both methods using starch from different species and tissues and demonstrated their effectiveness by screening for induced variation in starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules.					
36876784	7	30	theme	granule	1079:1085	arg1	size					1087:1090	starch granule size	1072:1090	starch granule size	1072:1090	Identifying variation in starch granule size and shape will enable identification of trait-controlling genes for developing crops with desired properties, and could help optimise starch processing.					
36876784	1	31	theme	biological	216:225	arg1	origin					227:232	biological origin	216:232	biological origin	216:232	Starch forms semi-crystalline, water-insoluble granules, the size and morphology of which vary according to biological origin.					
36876784	3	32	theme	granule	411:417	arg1	size					419:422	starch granule size	404:422	starch granule size	404:422	However, screening methods to identify differences in starch granule size and shape are lacking.					
36876784	5	33	from	variation	774:782	arg1	starch					787:792	starch	787:792	starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules	787:923	We evaluated the practicality of both methods using starch from different species and tissues and demonstrated their effectiveness by screening for induced variation in starch extracted from over 10,000 barley lines, yielding four with heritable changes in the ratio of large A-granules to small B-granules.					
36876784	4	34	dep	automated	573:581	arg1	high-throughput					584:598	high-throughput	584:598	high-throughput	584:598	Here, we present two approaches for high-throughput starch granule extraction and size determination using flow cytometry and automated, high-throughput light microscopy.					
36876784	6	35	theme	lines	950:954	arg1	Analysis					926:933	Analysis	926:933	Analysis of Arabidopsis lines altered in starch biosynthesis	926:985	Analysis of Arabidopsis lines altered in starch biosynthesis further demonstrates the applicability of these approaches.					
36876784	7	36	theme	desired	1182:1188	arg1	properties					1190:1199	desired properties	1182:1199	desired properties	1182:1199	Identifying variation in starch granule size and shape will enable identification of trait-controlling genes for developing crops with desired properties, and could help optimise starch processing.					
36876784	0	37	from	variation	10:18	arg1	size					38:41	starch granule size	23:41	starch granule size	23:41	Detecting variation in starch granule size and morphology by high-throughput microscopy and flow cytometry.					
36876784	0	37	from	variation	10:18	arg1	morphology					47:56	morphology	47:56	morphology	47:56	Detecting variation in starch granule size and morphology by high-throughput microscopy and flow cytometry.					
36876784	4	38	theme	flow	554:557	arg1	cytometry					559:567	flow cytometry	554:567	flow cytometry	554:567	Here, we present two approaches for high-throughput starch granule extraction and size determination using flow cytometry and automated, high-throughput light microscopy.					
36876784	3	39	dep	methods	369:375	arg1	identify					380:387	identify	380:387	to identify differences in starch granule size and shape	377:432	However, screening methods to identify differences in starch granule size and shape are lacking.					
36876784	2	40	theme	polymer	263:269	arg1	composition					271:281	polymer composition	263:281	polymer composition	263:281	These traits, together with polymer composition and structure, determine the physicochemical properties of starch.					
36876784	7	41	theme	starch	1072:1077	arg1	size					1087:1090	starch granule size	1072:1090	starch granule size	1072:1090	Identifying variation in starch granule size and shape will enable identification of trait-controlling genes for developing crops with desired properties, and could help optimise starch processing.					
36876784	2	42	theme	starch	342:347	arg1	properties					328:337	the physicochemical properties	308:337	the physicochemical properties of starch	308:347	These traits, together with polymer composition and structure, determine the physicochemical properties of starch.					
36876784	1	43	theme	water-insoluble	139:153	arg1	semi-crystalline					121:136	semi-crystalline	121:136	semi-crystalline	121:136	Starch forms semi-crystalline, water-insoluble granules, the size and morphology of which vary according to biological origin.					
36876784	1	43	theme	water-insoluble	139:153	arg1	granules					155:162	water-insoluble granules	139:162	water-insoluble granules	139:162	Starch forms semi-crystalline, water-insoluble granules, the size and morphology of which vary according to biological origin.					
36876784	7	44	with	crops	1171:1175	arg1	properties					1190:1199	desired properties	1182:1199	desired properties	1182:1199	Identifying variation in starch granule size and shape will enable identification of trait-controlling genes for developing crops with desired properties, and could help optimise starch processing.					
36876784	0	45	theme	high-throughput	61:75	arg1	microscopy					77:86	high-throughput microscopy	61:86	high-throughput microscopy	61:86	Detecting variation in starch granule size and morphology by high-throughput microscopy and flow cytometry.					
36876784	1	46	theme	granules	155:162	arg1	morphology					178:187	morphology	178:187	morphology	178:187	Starch forms semi-crystalline, water-insoluble granules, the size and morphology of which vary according to biological origin.					
36876784	1	46	theme	granules	155:162	arg1	size					169:172	size	169:172	size	169:172	Starch forms semi-crystalline, water-insoluble granules, the size and morphology of which vary according to biological origin.					
35575443	0	0	theme	activity	79:86	arg1	evaluation					46:55	evaluation	46:55	evaluation	46:55	Chitosan-biotin topical film: preparation and evaluation of burn wound healing activity.					
35575443	0	0	theme	activity	79:86	arg1	preparation					30:40	preparation	30:40	preparation	30:40	Chitosan-biotin topical film: preparation and evaluation of burn wound healing activity.					
35575443	3	1	theme	different	267:275	arg1	ratios					277:282	Four different ratios	262:282	Four different ratios of CS to BIO v/v (4:1, 3:2, 2:3, and 1:4)	262:324	Four different ratios of CS to BIO v/v (4:1, 3:2, 2:3, and 1:4) were prepared.					
35575443	5	2	theme	release	425:431	arg1	studies					433:439	The in-vitro release studies	412:439	The in-vitro release studies	412:439	The in-vitro release studies showed that the most promising formula with the highest release behavior was CS to BIO 1: 4.					
35575443	7	3	from	activities	774:783	arg1	model					822:826	the excision wound model	803:826	the excision wound model in mice	803:834	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	7	3	from	activities	774:783	arg1	model					850:854	skin burn model	840:854	skin burn model in rats	840:862	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	7	4	theme	skin	840:843	arg1	model					850:854	skin burn model	840:854	skin burn model in rats	840:862	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	7	5	from	model	850:854	arg1	rats					859:862	rats	859:862	rats	859:862	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	7	5	from	model	850:854	arg1	mice					831:834	mice	831:834	mice	831:834	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	7	5	from	model	850:854	arg1	activities					774:783	the skin healing activities	757:783	the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats	757:862	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	0	6	theme	healing	71:77	arg1	activity					79:86	burn wound healing activity	60:86	burn wound healing activity	60:86	Chitosan-biotin topical film: preparation and evaluation of burn wound healing activity.					
35575443	6	7	dep	ex	538:539	arg1	vivo					541:544	vivo	541:544	vivo	541:544	The ex vivo adhesion times were reported as 0.50 ± 0.30 min for CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively.					
35575443	1	8	from	biotin	133:138	arg1	film					105:108	A new composite film	89:108	A new composite film from chitosan (CS) and biotin (BIO)	89:144	A new composite film from chitosan (CS) and biotin (BIO) was developed to enhance burn wound healing.					
35575443	7	9	theme	film	795:798	arg1	activities					774:783	the skin healing activities	757:783	the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats	757:862	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	8	10	theme	skin	936:939	arg1	stretching					941:950	skin stretching	936:950	skin stretching	936:950	Furthermore, skin stretching and burn wound contraction behavior treated with CS/BIO were higher than that of CS treated skin.					
35575443	9	11	theme	superior	1123:1130	arg1	activity					1151:1158	superior burn wound healing activity	1123:1158	superior burn wound healing activity	1123:1158	In conclusion, the results obtained revealed that CS/BIO films possessed superior burn wound healing activity compared to CS.					
35575443	5	12	theme	release	497:503	arg1	behavior					505:512	the highest release behavior	485:512	the highest release behavior	485:512	The in-vitro release studies showed that the most promising formula with the highest release behavior was CS to BIO 1: 4.					
35575443	8	13	theme	burn	956:959	arg1	behavior					979:986	burn wound contraction behavior	956:986	burn wound contraction behavior	956:986	Furthermore, skin stretching and burn wound contraction behavior treated with CS/BIO were higher than that of CS treated skin.					
35575443	7	14	theme	healing	766:772	arg1	activities					774:783	the skin healing activities	757:783	the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats	757:862	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	3	15	theme	BIO	293:295	arg1	v/v					297:299	BIO v/v	293:299	BIO v/v (4:1, 3:2, 2:3, and 1:4)	293:324	Four different ratios of CS to BIO v/v (4:1, 3:2, 2:3, and 1:4) were prepared.					
35575443	7	16	theme	healing	887:893	arg1	rates					878:882	faster rates	871:882	faster rates of healing	871:893	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	1	17	from	chitosan	115:122	arg1	film					105:108	A new composite film	89:108	A new composite film from chitosan (CS) and biotin (BIO)	89:144	A new composite film from chitosan (CS) and biotin (BIO) was developed to enhance burn wound healing.					
35575443	2	18	theme	electrostatic	216:228	arg1	interaction					230:240	electrostatic interaction	216:240	electrostatic interaction between CS and BIO	216:259	The film was prepared by electrostatic interaction between CS and BIO.					
35575443	7	19	from	film	795:798	arg1	model					822:826	the excision wound model	803:826	the excision wound model in mice	803:834	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	7	19	from	film	795:798	arg1	model					850:854	skin burn model	840:854	skin burn model in rats	840:862	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	0	20	theme	topical	16:22	arg1	film					24:27	Chitosan-biotin topical film	0:27	Chitosan-biotin topical film: preparation and evaluation of burn wound healing activity.	0:87	Chitosan-biotin topical film: preparation and evaluation of burn wound healing activity.					
35575443	8	21	theme	wound	961:965	arg1	behavior					979:986	burn wound contraction behavior	956:986	burn wound contraction behavior	956:986	Furthermore, skin stretching and burn wound contraction behavior treated with CS/BIO were higher than that of CS treated skin.					
35575443	7	22	theme	faster	871:876	arg1	rates					878:882	faster rates	871:882	faster rates of healing	871:893	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	5	23	theme	promising	462:470	arg1	formula					472:478	the most promising formula	453:478	the most promising formula with the highest release behavior	453:512	The in-vitro release studies showed that the most promising formula with the highest release behavior was CS to BIO 1: 4.					
35575443	5	23	theme	promising	462:470	arg1	CS					518:519	CS	518:519	CS	518:519	The in-vitro release studies showed that the most promising formula with the highest release behavior was CS to BIO 1: 4.					
35575443	8	24	theme	CS	1033:1034	arg1	skin					1044:1047	CS treated skin	1033:1047	CS treated skin	1033:1047	Furthermore, skin stretching and burn wound contraction behavior treated with CS/BIO were higher than that of CS treated skin.					
35575443	0	25	theme	Chitosan-biotin	0:14	arg1	film					24:27	Chitosan-biotin topical film	0:27	Chitosan-biotin topical film: preparation and evaluation of burn wound healing activity.	0:87	Chitosan-biotin topical film: preparation and evaluation of burn wound healing activity.					
35575443	5	26	with	formula	472:478	arg1	behavior					505:512	the highest release behavior	485:512	the highest release behavior	485:512	The in-vitro release studies showed that the most promising formula with the highest release behavior was CS to BIO 1: 4.					
35575443	6	27	theme	adhesion	546:553	arg1	0.50 ± 0.30 min					578:592	0.50 ± 0.30 min	578:592	0.50 ± 0.30 min	578:592	The ex vivo adhesion times were reported as 0.50 ± 0.30 min for CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively.					
35575443	6	27	theme	adhesion	546:553	arg1	times					555:559	The ex vivo adhesion times	534:559	The ex vivo adhesion times	534:559	The ex vivo adhesion times were reported as 0.50 ± 0.30 min for CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively.					
35575443	7	28	theme	skin	761:764	arg1	healing					766:772	skin healing	761:772	the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats	757:862	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	1	29	theme	new	91:93	arg1	film					105:108	A new composite film	89:108	A new composite film from chitosan (CS) and biotin (BIO)	89:144	A new composite film from chitosan (CS) and biotin (BIO) was developed to enhance burn wound healing.					
35575443	6	30	dep	v/v	696:698	arg1	1:4					701:703	1:4	701:703	1:4	701:703	The ex vivo adhesion times were reported as 0.50 ± 0.30 min for CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively.					
35575443	6	30	dep	v/v	696:698	arg1	4:1					719:721	4:1	719:721	4:1	719:721	The ex vivo adhesion times were reported as 0.50 ± 0.30 min for CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively.					
35575443	6	30	dep	v/v	696:698	arg1	3:2					711:713	3:2	711:713	3:2	711:713	The ex vivo adhesion times were reported as 0.50 ± 0.30 min for CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively.					
35575443	6	30	dep	v/v	696:698	arg1	2:3					706:708	2:3	706:708	2:3	706:708	The ex vivo adhesion times were reported as 0.50 ± 0.30 min for CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively.					
35575443	9	31	contain	possessed	1113:1121	arg1	films					1107:1111	CS/BIO films	1100:1111	CS/BIO films	1100:1111	In conclusion, the results obtained revealed that CS/BIO films possessed superior burn wound healing activity compared to CS.					
35575443	9	31	contain	possessed	1113:1121	arg2	activity					1151:1158	superior burn wound healing activity	1123:1158	superior burn wound healing activity	1123:1158	In conclusion, the results obtained revealed that CS/BIO films possessed superior burn wound healing activity compared to CS.					
35575443	1	32	theme	composite	95:103	arg1	film					105:108	A new composite film	89:108	A new composite film from chitosan (CS) and biotin (BIO)	89:144	A new composite film from chitosan (CS) and biotin (BIO) was developed to enhance burn wound healing.					
35575443	6	33	theme	ex	538:539	arg1	0.50 ± 0.30 min					578:592	0.50 ± 0.30 min	578:592	0.50 ± 0.30 min	578:592	The ex vivo adhesion times were reported as 0.50 ± 0.30 min for CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively.					
35575443	6	33	theme	ex	538:539	arg1	times					555:559	The ex vivo adhesion times	534:559	The ex vivo adhesion times	534:559	The ex vivo adhesion times were reported as 0.50 ± 0.30 min for CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively.					
35575443	8	34	theme	treated	1036:1042	arg1	skin					1044:1047	CS treated skin	1033:1047	CS treated skin	1033:1047	Furthermore, skin stretching and burn wound contraction behavior treated with CS/BIO were higher than that of CS treated skin.					
35575443	9	35	theme	burn	1132:1135	arg1	healing					1143:1149	burn wound healing	1132:1149	superior burn wound healing activity	1123:1158	In conclusion, the results obtained revealed that CS/BIO films possessed superior burn wound healing activity compared to CS.					
35575443	7	36	from	model	822:826	arg1	rats					859:862	rats	859:862	rats	859:862	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	7	36	from	model	822:826	arg1	mice					831:834	mice	831:834	mice	831:834	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	7	36	from	model	822:826	arg1	activities					774:783	the skin healing activities	757:783	the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats	757:862	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	9	37	theme	wound	1137:1141	arg1	healing					1143:1149	burn wound healing	1132:1149	superior burn wound healing activity	1123:1158	In conclusion, the results obtained revealed that CS/BIO films possessed superior burn wound healing activity compared to CS.					
35575443	1	38	theme	burn	171:174	arg1	healing					182:188	burn wound healing	171:188	burn wound healing	171:188	A new composite film from chitosan (CS) and biotin (BIO) was developed to enhance burn wound healing.					
35575443	9	39	theme	healing	1143:1149	arg1	activity					1151:1158	superior burn wound healing activity	1123:1158	superior burn wound healing activity	1123:1158	In conclusion, the results obtained revealed that CS/BIO films possessed superior burn wound healing activity compared to CS.					
35575443	6	40	theme	13.83 ± 1.04 min	659:674	arg1	v/v					696:698	13.83 ± 1.04 min for CS to BIO films v/v	659:698	13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1)	659:722	The ex vivo adhesion times were reported as 0.50 ± 0.30 min for CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively.					
35575443	0	41	theme	burn	60:63	arg1	healing					71:77	burn wound healing	60:77	burn wound healing activity	60:86	Chitosan-biotin topical film: preparation and evaluation of burn wound healing activity.					
35575443	7	42	theme	burn	845:848	arg1	model					850:854	skin burn model	840:854	skin burn model in rats	840:862	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	7	43	theme	excision	807:814	arg1	model					822:826	the excision wound model	803:826	the excision wound model in mice	803:834	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	3	44	dep	v/v	297:299	arg1	3:2					307:309	3:2	307:309	3:2	307:309	Four different ratios of CS to BIO v/v (4:1, 3:2, 2:3, and 1:4) were prepared.					
35575443	3	44	dep	v/v	297:299	arg1	4:1					302:304	4:1	302:304	4:1	302:304	Four different ratios of CS to BIO v/v (4:1, 3:2, 2:3, and 1:4) were prepared.					
35575443	3	44	dep	v/v	297:299	arg1	2:3					312:314	2:3	312:314	2:3	312:314	Four different ratios of CS to BIO v/v (4:1, 3:2, 2:3, and 1:4) were prepared.					
35575443	3	44	dep	v/v	297:299	arg1	1:4					321:323	1:4	321:323	1:4	321:323	Four different ratios of CS to BIO v/v (4:1, 3:2, 2:3, and 1:4) were prepared.					
35575443	5	45	theme	highest	489:495	arg1	behavior					505:512	the highest release behavior	485:512	the highest release behavior	485:512	The in-vitro release studies showed that the most promising formula with the highest release behavior was CS to BIO 1: 4.					
35575443	7	46	theme	CS/BIO	788:793	arg1	film					795:798	CS/BIO film	788:798	CS/BIO film in the excision wound model in mice and skin burn model in rats	788:862	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	1	47	theme	wound	176:180	arg1	healing					182:188	burn wound healing	171:188	burn wound healing	171:188	A new composite film from chitosan (CS) and biotin (BIO) was developed to enhance burn wound healing.					
35575443	7	48	theme	wound	816:820	arg1	model					822:826	the excision wound model	803:826	the excision wound model in mice	803:834	Most importantly, the skin healing activities of CS/BIO film in the excision wound model in mice and skin burn model in rats showed faster rates of healing compared to CS and placebo.					
35575443	6	49	theme	BIO	686:688	arg1	films					690:694	BIO films	686:694	BIO films	686:694	The ex vivo adhesion times were reported as 0.50 ± 0.30 min for CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively.					
35575443	0	50	dep	film	24:27	arg1	evaluation					46:55	evaluation	46:55	evaluation	46:55	Chitosan-biotin topical film: preparation and evaluation of burn wound healing activity.					
35575443	0	50	dep	film	24:27	arg1	preparation					30:40	preparation	30:40	preparation	30:40	Chitosan-biotin topical film: preparation and evaluation of burn wound healing activity.					
35575443	0	51	theme	wound	65:69	arg1	healing					71:77	burn wound healing	60:77	burn wound healing activity	60:86	Chitosan-biotin topical film: preparation and evaluation of burn wound healing activity.					
35575443	9	52	theme	CS/BIO	1100:1105	arg1	films					1107:1111	CS/BIO films	1100:1111	CS/BIO films	1100:1111	In conclusion, the results obtained revealed that CS/BIO films possessed superior burn wound healing activity compared to CS.					
35575443	6	53	theme	CS	598:599	arg1	film					601:604	CS film	598:604	CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively	598:736	The ex vivo adhesion times were reported as 0.50 ± 0.30 min for CS film compared to 6.2 ± 0.30, 8.4 ± 0.40, 11.2 ± 0.50, and 13.83 ± 1.04 min for CS to BIO films v/v (1:4, 2:3, 3:2 and 4:1), respectively.					
35575443	3	54	theme	CS	287:288	arg1	ratios					277:282	Four different ratios	262:282	Four different ratios of CS to BIO v/v (4:1, 3:2, 2:3, and 1:4)	262:324	Four different ratios of CS to BIO v/v (4:1, 3:2, 2:3, and 1:4) were prepared.					
35575443	5	55	theme	in-vitro	416:423	arg1	studies					433:439	The in-vitro release studies	412:439	The in-vitro release studies	412:439	The in-vitro release studies showed that the most promising formula with the highest release behavior was CS to BIO 1: 4.					
35575443	8	56	theme	contraction	967:977	arg1	behavior					979:986	burn wound contraction behavior	956:986	burn wound contraction behavior	956:986	Furthermore, skin stretching and burn wound contraction behavior treated with CS/BIO were higher than that of CS treated skin.					
37330080	8	0	theme	first	1772:1776	arg1	study					1759:1763	presented study	1749:1763	presented study	1749:1763	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	8	0	theme	first	1772:1776	arg1	report					1778:1783	the first report	1768:1783	the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material	1768:2037	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	3	1	theme	grape	544:548	arg1	GP					558:559	GP	558:559	GP	558:559	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	3	1	theme	grape	544:548	arg1	pomace					550:555	grape pomace	544:555	grape pomace (GP) hydrolysate	544:572	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	4	2	used	used	716:719	arg2	CCD					707:709	CCD	707:709	CCD	707:709	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	4	2	used	used	716:719	arg2	design					699:704	The central composite design	677:704	The central composite design (CCD)	677:710	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	7	3	theme	%	1419:1419	arg1	reduction					1421:1429	a complex RAE medium 34 % reduction	1395:1429	a complex RAE medium 34 % reduction of crystallinity index	1395:1452	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	1	4	theme	industrial	298:307	arg1	applications					309:320	various industrial applications	290:320	various industrial applications	290:320	The microbial production of cellulose using different bacterial species has been extensively examined for various industrial applications.					
37330080	7	5	theme	medium	1409:1414	arg1	reduction					1421:1429	a complex RAE medium 34 % reduction	1395:1429	a complex RAE medium 34 % reduction of crystallinity index	1395:1452	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	4	6	theme	minimal	822:828	arg1	4.8 g/L					849:855	4.8 g/L	849:855	4.8 g/L	849:855	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	4	6	theme	minimal	822:828	arg1	contents					839:846	minimal phenolic contents	822:846	minimal phenolic contents (4.8 g/L)	822:856	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	6	7	theme	bacteria	1219:1226	arg1	4 days					1209:1214	only 4 days	1204:1214	only 4 days of bacteria culturing, 1 st day with shaking	1204:1259	The membranes were synthesized in only 4 days of bacteria culturing, 1 st day with shaking, followed by 3 days of static incubation.					
37330080	1	8	theme	microbial	188:196	arg1	production					198:207	The microbial production	184:207	The microbial production of cellulose using different bacterial species	184:254	The microbial production of cellulose using different bacterial species has been extensively examined for various industrial applications.					
37330080	7	9	theme	complex	1397:1403	arg1	reduction					1421:1429	a complex RAE medium 34 % reduction	1395:1429	a complex RAE medium 34 % reduction of crystallinity index	1395:1452	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	10	theme	tensile	1697:1703	arg1	modulus					1705:1711	tensile modulus	1697:1711	tensile modulus	1697:1711	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	11	theme	hydrophobicity	1593:1606	arg1	reduction					1613:1621	the reduction	1609:1621	the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength	1609:1694	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	11	theme	hydrophobicity	1593:1606	arg1	increase					1581:1588	the increase	1577:1588	the increase of hydrophobicity	1577:1606	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	11	theme	hydrophobicity	1593:1606	arg1	modulus					1705:1711	tensile modulus	1697:1711	tensile modulus	1697:1711	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	11	theme	hydrophobicity	1593:1606	arg1	elongation					1718:1727	elongation	1718:1727	elongation	1718:1727	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	6	12	theme	static	1284:1289	arg1	incubation					1291:1300	static incubation	1284:1300	static incubation	1284:1300	The membranes were synthesized in only 4 days of bacteria culturing, 1 st day with shaking, followed by 3 days of static incubation.					
37330080	3	13	theme	enzymatic	583:591	arg1	treatment					593:601	enzymatic treatment	583:601	enzymatic treatment	583:601	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	5	14	dep	species	978:984	arg1	AV436T					1015:1020	AV436T	1015:1020	AV436T	1015:1020	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	15	theme	dry	1072:1074	arg1	membrane					1079:1086	up to 1.24 g/L dry BC membrane	1057:1086	up to 1.24 g/L dry BC membrane	1057:1086	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	15	theme	dry	1072:1074	arg1	producer					1047:1054	the most efficient BC producer	1025:1054	the most efficient BC producer (up to 1.24 g/L dry BC membrane)	1025:1087	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	3	16	theme	growth	614:619	arg1	hydrolysate					562:572	grape pomace (GP) hydrolysate	544:572	grape pomace (GP) hydrolysate	544:572	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	3	16	theme	growth	614:619	arg1	medium					621:626	a sole growth medium	607:626	a sole growth medium for BC production by acetic acid bacteria (AAB)	607:674	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	1	17	theme	bacterial	238:246	arg1	species					248:254	different bacterial species	228:254	different bacterial species	228:254	The microbial production of cellulose using different bacterial species has been extensively examined for various industrial applications.					
37330080	8	18	theme	culture	1853:1859	arg1	medium					1861:1866	a sole culture medium	1846:1866	a sole culture medium for efficient BC production by AAB	1846:1901	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	8	18	theme	culture	1853:1859	arg1	GP-hydrolysate					1800:1813	a GP-hydrolysate	1798:1813	a GP-hydrolysate without enzymatic treatment	1798:1841	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	5	19	theme	BC	1044:1045	arg1	membrane					1079:1086	up to 1.24 g/L dry BC membrane	1057:1086	up to 1.24 g/L dry BC membrane	1057:1086	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	19	theme	BC	1044:1045	arg1	species					978:984	the recently described species	955:984	the recently described species Komagataeibacter melomenusus AV436T	955:1020	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	19	theme	BC	1044:1045	arg1	producer					1047:1054	the most efficient BC producer	1025:1054	the most efficient BC producer (up to 1.24 g/L dry BC membrane)	1025:1087	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	7	20	dep	membranes	1377:1385	arg1	comparison					1359:1368	comparison	1359:1368	comparison	1359:1368	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	21	theme	produced	1307:1314	arg1	membranes					1319:1327	The produced BC membranes	1303:1327	The produced BC membranes in GP-hydrolysates	1303:1346	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	9	22	theme	production	2138:2147	arg1	cost-optimisation					2114:2130	the cost-optimisation	2110:2130	the cost-optimisation of BC production at the industrial levels	2110:2172	The scale-up protocol of the scheme presented here will be needed for the cost-optimisation of BC production at the industrial levels.					
37330080	5	23	theme	hydrolysates	912:923	arg1	screening					876:884	The experimental screening	859:884	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains	859:942	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	6	24	dep	synthesized	1189:1199	arg1	followed					1262:1269	followed	1262:1269	followed by 3 days of static incubation	1262:1300	The membranes were synthesized in only 4 days of bacteria culturing, 1 st day with shaking, followed by 3 days of static incubation.					
37330080	8	25	theme	BC	1882:1883	arg1	production					1885:1894	efficient BC production	1872:1894	efficient BC production by AAB	1872:1901	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	5	26	theme	experimental	863:874	arg1	screening					876:884	The experimental screening	859:884	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains	859:942	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	7	27	theme	components	1528:1537	arg1	presence					1505:1512	presence	1505:1512	presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively	1505:1741	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	0	28	theme	novel	75:79	arg1	hydrolysate					94:104	a novel grape pomace hydrolysate	73:104	a novel grape pomace hydrolysate	73:104	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	5	29	theme	xylinus	1119:1125	arg1	1518					1131:1134	Komagataeibacter xylinus LMG 1518	1102:1134	Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane)	1102:1167	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	29	theme	xylinus	1119:1125	arg1	membrane					1159:1166	up to 0.98 g/L dry BC membrane	1137:1166	up to 0.98 g/L dry BC membrane	1137:1166	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	7	30	theme	%	1654:1654	arg1	reduction					1613:1621	the reduction	1609:1621	the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength	1609:1694	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	30	theme	%	1654:1654	arg1	increase					1581:1588	the increase	1577:1588	the increase of hydrophobicity	1577:1606	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	30	theme	%	1654:1654	arg1	modulus					1705:1711	tensile modulus	1697:1711	tensile modulus	1697:1711	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	30	theme	%	1654:1654	arg1	elongation					1718:1727	elongation	1718:1727	elongation	1718:1727	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	0	31	from	Production	0:9	arg1	hydrolysate					94:104	a novel grape pomace hydrolysate	73:104	a novel grape pomace hydrolysate	73:104	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	4	32	theme	hydrolysate	740:750	arg1	preparation					752:762	the GP hydrolysate preparation	733:762	the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L)	733:856	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	0	33	theme	pomace	87:92	arg1	hydrolysate					94:104	a novel grape pomace hydrolysate	73:104	a novel grape pomace hydrolysate	73:104	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	2	34	theme	bacterial	441:449	arg1	production					466:475	bacterial cellulose (BC) production	441:475	bacterial cellulose (BC) production	441:475	However, the cost-effectiveness of all these biotechnological processes is strongly related to the culture medium for bacterial cellulose (BC) production.					
37330080	5	35	theme	described	968:976	arg1	species					978:984	the recently described species	955:984	the recently described species Komagataeibacter melomenusus AV436T	955:1020	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	35	theme	described	968:976	arg1	producer					1047:1054	the most efficient BC producer	1025:1054	the most efficient BC producer (up to 1.24 g/L dry BC membrane)	1025:1087	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	7	36	theme	thermal	1626:1632	arg1	stability					1634:1642	thermal stability	1626:1642	thermal stability	1626:1642	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	37	from	membranes	1319:1327	arg1	GP-hydrolysates					1332:1346	GP-hydrolysates	1332:1346	GP-hydrolysates	1332:1346	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	5	38	theme	BC	1156:1157	arg1	1518					1131:1134	Komagataeibacter xylinus LMG 1518	1102:1134	Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane)	1102:1167	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	38	theme	BC	1156:1157	arg1	membrane					1159:1166	up to 0.98 g/L dry BC membrane	1137:1166	up to 0.98 g/L dry BC membrane	1137:1166	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	7	39	theme	diverse	1475:1481	arg1	allomorphs					1493:1502	diverse cellulose allomorphs	1475:1502	diverse cellulose allomorphs	1475:1502	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	5	40	theme	strains	936:942	arg1	screening					876:884	The experimental screening	859:884	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains	859:942	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	4	41	theme	central	681:687	arg1	CCD					707:709	CCD	707:709	CCD	707:709	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	4	41	theme	central	681:687	arg1	design					699:704	The central composite design	677:704	The central composite design (CCD)	677:710	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	8	42	theme	material	2030:2037	arg1	type					2011:2014	this type	2006:2014	this type of food-waste material	2006:2037	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	3	43	theme	acetic	649:654	arg1	AAB					671:673	AAB	671:673	AAB	671:673	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	3	43	theme	acetic	649:654	arg1	bacteria					661:668	acetic acid bacteria	649:668	acetic acid bacteria (AAB)	649:674	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	0	44	theme	cellulose	50:58	arg1	membranes					60:68	bacterial cellulose membranes	40:68	bacterial cellulose membranes in a novel grape pomace hydrolysate	40:104	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	5	45	theme	efficient	1034:1042	arg1	membrane					1079:1086	up to 1.24 g/L dry BC membrane	1057:1086	up to 1.24 g/L dry BC membrane	1057:1086	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	45	theme	efficient	1034:1042	arg1	species					978:984	the recently described species	955:984	the recently described species Komagataeibacter melomenusus AV436T	955:1020	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	45	theme	efficient	1034:1042	arg1	producer					1047:1054	the most efficient BC producer	1025:1054	the most efficient BC producer (up to 1.24 g/L dry BC membrane)	1025:1087	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	4	46	theme	highest	775:781	arg1	10.4 g/L					808:815	10.4 g/L	808:815	10.4 g/L	808:815	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	4	46	theme	highest	775:781	arg1	contents					798:805	the highest reducing sugar contents	771:805	the highest reducing sugar contents (10.4 g/L)	771:816	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	2	47	theme	biotechnological	368:383	arg1	processes					385:393	all these biotechnological processes	358:393	all these biotechnological processes	358:393	However, the cost-effectiveness of all these biotechnological processes is strongly related to the culture medium for bacterial cellulose (BC) production.					
37330080	7	48	theme	crystallinity	1434:1446	arg1	index					1448:1452	crystallinity index	1434:1452	crystallinity index	1434:1452	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	4	49	theme	sugar	792:796	arg1	10.4 g/L					808:815	10.4 g/L	808:815	10.4 g/L	808:815	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	4	49	theme	sugar	792:796	arg1	contents					798:805	the highest reducing sugar contents	771:805	the highest reducing sugar contents (10.4 g/L)	771:816	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	3	50	theme	pomace	550:555	arg1	hydrolysate					562:572	grape pomace (GP) hydrolysate	544:572	grape pomace (GP) hydrolysate	544:572	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	3	50	theme	pomace	550:555	arg1	medium					621:626	a sole growth medium	607:626	a sole growth medium for BC production by acetic acid bacteria (AAB)	607:674	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	6	51	dep	4 days	1209:1214	arg1	day					1244:1246	culturing, 1 st day	1228:1246	day	1244:1246	The membranes were synthesized in only 4 days of bacteria culturing, 1 st day with shaking, followed by 3 days of static incubation.					
37330080	0	52	dep	Production	0:9	arg1	efficiency					11:20	efficiency	11:20	efficiency	11:20	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	5	53	dep	1.24 g/L	1063:1070	arg1	to					1060:1061	to	1060:1061	to	1060:1061	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	9	54	theme	scale-up	2044:2051	arg1	protocol					2053:2060	The scale-up protocol	2040:2060	The scale-up protocol of the scheme presented here	2040:2089	The scale-up protocol of the scheme presented here will be needed for the cost-optimisation of BC production at the industrial levels.					
37330080	6	55	with	4 days	1209:1214	arg1	shaking					1253:1259	shaking	1253:1259	shaking	1253:1259	The membranes were synthesized in only 4 days of bacteria culturing, 1 st day with shaking, followed by 3 days of static incubation.					
37330080	7	56	theme	34 	1416:1418	arg1	reduction					1421:1429	a complex RAE medium 34 % reduction	1395:1429	a complex RAE medium 34 % reduction of crystallinity index	1395:1452	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	8	57	theme	efficient	1984:1992	arg1	producer					1994:2001	the most efficient producer	1975:2001	the most efficient producer in this type of food-waste material	1975:2037	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	6	58	theme	1 st	1239:1242	arg1	day					1244:1246	culturing, 1 st day	1228:1246	day	1244:1246	The membranes were synthesized in only 4 days of bacteria culturing, 1 st day with shaking, followed by 3 days of static incubation.					
37330080	1	59	theme	various	290:296	arg1	applications					309:320	various industrial applications	290:320	various industrial applications	290:320	The microbial production of cellulose using different bacterial species has been extensively examined for various industrial applications.					
37330080	7	60	theme	RAE	1405:1407	arg1	reduction					1421:1429	a complex RAE medium 34 % reduction	1395:1429	a complex RAE medium 34 % reduction of crystallinity index	1395:1452	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	9	61	theme	scheme	2069:2074	arg1	protocol					2053:2060	The scale-up protocol	2040:2060	The scale-up protocol of the scheme presented here	2040:2089	The scale-up protocol of the scheme presented here will be needed for the cost-optimisation of BC production at the industrial levels.					
37330080	6	62	theme	culturing	1228:1236	arg1	day					1244:1246	culturing, 1 st day	1228:1246	day	1244:1246	The membranes were synthesized in only 4 days of bacteria culturing, 1 st day with shaking, followed by 3 days of static incubation.					
37330080	8	63	theme	enzymatic	1823:1831	arg1	treatment					1833:1841	enzymatic treatment	1823:1841	enzymatic treatment	1823:1841	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	4	64	theme	phenolic	830:837	arg1	4.8 g/L					849:855	4.8 g/L	849:855	4.8 g/L	849:855	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	4	64	theme	phenolic	830:837	arg1	contents					839:846	minimal phenolic contents	822:846	minimal phenolic contents (4.8 g/L)	822:856	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	7	65	theme	tensile	1679:1685	arg1	strength					1687:1694	lower tensile strength	1673:1694	lower tensile strength	1673:1694	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	6	66	theme	incubation	1291:1300	arg1	3 days					1274:1279	3 days	1274:1279	3 days of static incubation	1274:1300	The membranes were synthesized in only 4 days of bacteria culturing, 1 st day with shaking, followed by 3 days of static incubation.					
37330080	1	67	theme	cellulose	212:220	arg1	production					198:207	The microbial production	184:207	The microbial production of cellulose using different bacterial species	184:254	The microbial production of cellulose using different bacterial species has been extensively examined for various industrial applications.					
37330080	8	68	theme	sole	1848:1851	arg1	medium					1861:1866	a sole culture medium	1846:1866	a sole culture medium for efficient BC production by AAB	1846:1901	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	8	68	theme	sole	1848:1851	arg1	GP-hydrolysate					1800:1813	a GP-hydrolysate	1798:1813	a GP-hydrolysate without enzymatic treatment	1798:1841	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	3	69	theme	sole	609:612	arg1	hydrolysate					562:572	grape pomace (GP) hydrolysate	544:572	grape pomace (GP) hydrolysate	544:572	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	3	69	theme	sole	609:612	arg1	medium					621:626	a sole growth medium	607:626	a sole growth medium for BC production by acetic acid bacteria (AAB)	607:674	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	1	70	theme	different	228:236	arg1	species					248:254	different bacterial species	228:254	different bacterial species	228:254	The microbial production of cellulose using different bacterial species has been extensively examined for various industrial applications.					
37330080	7	71	theme	responsible	1561:1571	arg1	network					1553:1559	the BC network	1546:1559	the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively	1546:1741	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	5	72	theme	BC	1076:1077	arg1	membrane					1079:1086	up to 1.24 g/L dry BC membrane	1057:1086	up to 1.24 g/L dry BC membrane	1057:1086	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	72	theme	BC	1076:1077	arg1	producer					1047:1054	the most efficient BC producer	1025:1054	the most efficient BC producer (up to 1.24 g/L dry BC membrane)	1025:1087	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	7	73	theme	BC	1550:1551	arg1	network					1553:1559	the BC network	1546:1559	the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively	1546:1741	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	9	74	theme	BC	2135:2136	arg1	production					2138:2147	BC production	2135:2147	BC production	2135:2147	The scale-up protocol of the scheme presented here will be needed for the cost-optimisation of BC production at the industrial levels.					
37330080	8	75	theme	efficient	1872:1880	arg1	production					1885:1894	efficient BC production	1872:1894	efficient BC production by AAB	1872:1901	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	3	76	theme	BC	632:633	arg1	production					635:644	BC production	632:644	BC production by acetic acid bacteria (AAB)	632:674	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	5	77	theme	prepared	903:910	arg1	hydrolysates					912:923	4 differently prepared hydrolysates	889:923	4 differently prepared hydrolysates	889:923	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	7	78	theme	lower	1673:1677	arg1	strength					1687:1694	lower tensile strength	1673:1694	lower tensile strength	1673:1694	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	8	79	dep	species	1928:1934	arg1	AV436T					1965:1970	AV436T	1965:1970	AV436T	1965:1970	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	0	80	from	hydrolysate	94:104	arg1	Production					0:9	Production efficiency and properties	0:35	Production	0:9	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	0	80	from	hydrolysate	94:104	arg1	properties					26:35	properties	26:35	properties	26:35	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	7	81	theme	BC	1316:1317	arg1	membranes					1319:1327	The produced BC membranes	1303:1327	The produced BC membranes in GP-hydrolysates	1303:1346	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	82	theme	GP-related	1517:1526	arg1	components					1528:1537	GP-related components	1517:1537	GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively	1517:1741	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	0	83	from	properties	26:35	arg1	hydrolysate					94:104	a novel grape pomace hydrolysate	73:104	a novel grape pomace hydrolysate	73:104	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	9	84	theme	industrial	2156:2165	arg1	levels					2167:2172	the industrial levels	2152:2172	the industrial levels	2152:2172	The scale-up protocol of the scheme presented here will be needed for the cost-optimisation of BC production at the industrial levels.					
37330080	0	85	theme	grape	81:85	arg1	hydrolysate					94:104	a novel grape pomace hydrolysate	73:104	a novel grape pomace hydrolysate	73:104	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	5	86	theme	Komagataeibacter	1102:1117	arg1	1518					1131:1134	Komagataeibacter xylinus LMG 1518	1102:1134	Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane)	1102:1167	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	86	theme	Komagataeibacter	1102:1117	arg1	membrane					1159:1166	up to 0.98 g/L dry BC membrane	1137:1166	up to 0.98 g/L dry BC membrane	1137:1166	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	87	dep	0.98 g/L	1143:1150	arg1	to					1140:1141	to	1140:1141	to	1140:1141	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	4	88	theme	composite	689:697	arg1	CCD					707:709	CCD	707:709	CCD	707:709	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	4	88	theme	composite	689:697	arg1	design					699:704	The central composite design	677:704	The central composite design (CCD)	677:710	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	5	89	theme	LMG	1127:1129	arg1	1518					1131:1134	Komagataeibacter xylinus LMG 1518	1102:1134	Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane)	1102:1167	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	89	theme	LMG	1127:1129	arg1	membrane					1159:1166	up to 0.98 g/L dry BC membrane	1137:1166	up to 0.98 g/L dry BC membrane	1137:1166	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	7	90	theme	allomorphs	1493:1502	arg1	presence					1463:1470	the presence	1459:1470	the presence of diverse cellulose allomorphs	1459:1502	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	0	91	from	membranes	60:68	arg1	hydrolysate					94:104	a novel grape pomace hydrolysate	73:104	a novel grape pomace hydrolysate	73:104	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	4	92	theme	GP	737:738	arg1	preparation					752:762	the GP hydrolysate preparation	733:762	the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L)	733:856	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	8	93	theme	described	1918:1926	arg1	species					1928:1934	recently described species	1909:1934	recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material	1909:2037	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	7	94	dep	%	1654:1654	arg1	strength					1687:1694	lower tensile strength	1673:1694	lower tensile strength	1673:1694	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	8	95	theme	presented	1749:1757	arg1	study					1759:1763	presented study	1749:1763	presented study	1749:1763	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	8	95	theme	presented	1749:1757	arg1	report					1778:1783	the first report	1768:1783	the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material	1768:2037	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	7	96	theme	stability	1634:1642	arg1	reduction					1613:1621	the reduction	1609:1621	the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength	1609:1694	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	96	theme	stability	1634:1642	arg1	increase					1581:1588	the increase	1577:1588	the increase of hydrophobicity	1577:1606	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	96	theme	stability	1634:1642	arg1	modulus					1705:1711	tensile modulus	1697:1711	tensile modulus	1697:1711	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	7	96	theme	stability	1634:1642	arg1	elongation					1718:1727	elongation	1718:1727	elongation	1718:1727	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	9	97	from	levels	2167:2172	arg1	cost-optimisation					2114:2130	the cost-optimisation	2110:2130	the cost-optimisation of BC production at the industrial levels	2110:2172	The scale-up protocol of the scheme presented here will be needed for the cost-optimisation of BC production at the industrial levels.					
37330080	2	98	theme	culture	422:428	arg1	medium					430:435	the culture medium	418:435	the culture medium for bacterial cellulose (BC) production	418:475	However, the cost-effectiveness of all these biotechnological processes is strongly related to the culture medium for bacterial cellulose (BC) production.					
37330080	5	99	theme	dry	1152:1154	arg1	1518					1131:1134	Komagataeibacter xylinus LMG 1518	1102:1134	Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane)	1102:1167	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	99	theme	dry	1152:1154	arg1	membrane					1159:1166	up to 0.98 g/L dry BC membrane	1137:1166	up to 0.98 g/L dry BC membrane	1137:1166	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	5	100	theme	AAB	932:934	arg1	strains					936:942	20 AAB strains	929:942	20 AAB strains	929:942	The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane).					
37330080	8	101	theme	food-waste	2019:2028	arg1	material					2030:2037	food-waste material	2019:2037	food-waste material	2019:2037	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	3	102	theme	acid	656:659	arg1	AAB					671:673	AAB	671:673	AAB	671:673	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	3	102	theme	acid	656:659	arg1	bacteria					661:668	acetic acid bacteria	649:668	acetic acid bacteria (AAB)	649:674	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	3	103	theme	simple	500:505	arg1	procedure					520:528	a simple and modified procedure	498:528	a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB)	498:674	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	0	104	theme	bacterial	40:48	arg1	membranes					60:68	bacterial cellulose membranes	40:68	bacterial cellulose membranes in a novel grape pomace hydrolysate	40:104	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	7	105	theme	cellulose	1483:1491	arg1	allomorphs					1493:1502	diverse cellulose allomorphs	1475:1502	diverse cellulose allomorphs	1475:1502	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	2	106	theme	cellulose	451:459	arg1	production					466:475	bacterial cellulose (BC) production	441:475	bacterial cellulose (BC) production	441:475	However, the cost-effectiveness of all these biotechnological processes is strongly related to the culture medium for bacterial cellulose (BC) production.					
37330080	0	107	theme	membranes	60:68	arg1	Production					0:9	Production efficiency and properties	0:35	Production	0:9	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	0	107	theme	membranes	60:68	arg1	properties					26:35	properties	26:35	properties	26:35	Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518.					
37330080	2	108	theme	processes	385:393	arg1	cost-effectiveness					336:353	the cost-effectiveness	332:353	the cost-effectiveness of all these biotechnological processes	332:393	However, the cost-effectiveness of all these biotechnological processes is strongly related to the culture medium for bacterial cellulose (BC) production.					
37330080	2	108	theme	processes	385:393	arg1	related					407:413	related	407:413	related	407:413	However, the cost-effectiveness of all these biotechnological processes is strongly related to the culture medium for bacterial cellulose (BC) production.					
37330080	8	109	from	producer	1994:2001	arg1	type					2011:2014	this type	2006:2014	this type of food-waste material	2006:2037	Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material.					
37330080	3	110	theme	modified	511:518	arg1	procedure					520:528	a simple and modified procedure	498:528	a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB)	498:674	Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB).					
37330080	7	111	theme	index	1448:1452	arg1	reduction					1421:1429	a complex RAE medium 34 % reduction	1395:1429	a complex RAE medium 34 % reduction of crystallinity index	1395:1452	The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively.					
37330080	4	112	theme	reducing	783:790	arg1	10.4 g/L					808:815	10.4 g/L	808:815	10.4 g/L	808:815	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37330080	4	112	theme	reducing	783:790	arg1	contents					798:805	the highest reducing sugar contents	771:805	the highest reducing sugar contents (10.4 g/L)	771:816	The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L).					
37178178	1	0	from	green	404:408	arg1	fluids					641:646	biological fluids	630:646	biological fluids	630:646	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	0	1	theme	GC-FID	200:205	arg1	analysis					207:214	GC-FID analysis	200:214	GC-FID analysis	200:214	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	0	2	from	fluids	184:189	arg1	extraction					129:138	the simultaneous extraction	112:138	the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis	112:214	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	1	3	theme	acid	279:282	arg1	nanofibers					227:236	Composite nanofibers	217:236	Composite nanofibers	217:236	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	3	theme	acid	279:282	arg1	nanoparticles					330:342	citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles	272:342	citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs)	272:374	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	3	theme	acid	279:282	arg1	NPs					371:373	PVA/CA/β-cyclodextrin/CuO NPs	345:373	PVA/CA/β-cyclodextrin/CuO NPs	345:373	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	3	4	theme	extraction	1096:1105	arg1	efficiency					1107:1116	high extraction efficiency	1091:1116	high extraction efficiency	1091:1116	Due to the presence of β-cyclodextrins and CuO NPs rich of functional groups on their surface, the nanofibers have high extraction efficiency.					
37178178	1	5	from	citalopram	589:598	arg1	fluids					641:646	biological fluids	630:646	biological fluids	630:646	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	6	theme	adsorbent	425:433	arg1	IMP					583:585	IMP	583:585	IMP	583:585	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	6	theme	adsorbent	425:433	arg1	nanofibers					227:236	Composite nanofibers	217:236	Composite nanofibers	217:236	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	6	theme	adsorbent	425:433	arg1	namely imipramine					564:580	efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine	415:580	efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP)	415:586	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	6	7	theme	relative	1350:1357	arg1	%					1392:1392	4.8 to 8.7%	1382:1392	4.8 to 8.7% (within-day, n = 4)	1382:1412	The relative standard deviation was 4.8 to 8.7% (within-day, n = 4) and 5.1 to 9.2% (between-day, n = 3) for 3 consecutive days.					
37178178	6	7	theme	relative	1350:1357	arg1	deviation					1368:1376	The relative standard deviation	1346:1376	The relative standard deviation	1346:1376	The relative standard deviation was 4.8 to 8.7% (within-day, n = 4) and 5.1 to 9.2% (between-day, n = 3) for 3 consecutive days.					
37178178	1	8	theme	Composite	217:225	arg1	green					404:408	a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine	395:619	green	404:408	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	8	theme	Composite	217:225	arg1	alcohol					257:263	polyvinyl alcohol	247:263	polyvinyl alcohol (PVA)	247:269	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	8	theme	Composite	217:225	arg1	nanoparticles					330:342	citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles	272:342	citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs)	272:374	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	8	theme	Composite	217:225	arg1	clozapine					611:619	clozapine	611:619	clozapine	611:619	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	8	theme	Composite	217:225	arg1	citalopram					589:598	citalopram	589:598	citalopram (CIT)	589:604	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	8	theme	Composite	217:225	arg1	namely imipramine					564:580	efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine	415:580	efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP)	415:586	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	8	theme	Composite	217:225	arg1	nanofibers					227:236	Composite nanofibers	217:236	Composite nanofibers	217:236	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	6	9	dep	9.2	1425:1427	arg1	to					1422:1423	to	1422:1423	to	1422:1423	The relative standard deviation was 4.8 to 8.7% (within-day, n = 4) and 5.1 to 9.2% (between-day, n = 3) for 3 consecutive days.					
37178178	7	10	theme	 excellent	1487:1496	arg1	clean-up					1498:1505	 excellent clean-up	1487:1505	 excellent clean-up	1487:1505	In addition, excellent clean-up was achieved which is a great advantage over other sample preparation methods.					
37178178	2	11	from	spectroscopy	815:826	arg1	results					724:730	the obtained results	711:730	the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD)	711:910	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	0	12	theme	drugs	164:168	arg1	extraction					129:138	the simultaneous extraction	112:138	the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis	112:214	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	6	13	theme	consecutive	1457:1467	arg1	days					1469:1472	3 consecutive days	1455:1472	3 consecutive days	1455:1472	The relative standard deviation was 4.8 to 8.7% (within-day, n = 4) and 5.1 to 9.2% (between-day, n = 3) for 3 consecutive days.					
37178178	2	14	theme	successful	917:926	arg1	approved					966:973	approved	966:973	approved	966:973	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	14	theme	successful	917:926	arg1	synthesis					928:936	the successful synthesis	913:936	the successful synthesis of composite nanofibers	913:960	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	1	15	theme	biological	630:639	arg1	fluids					641:646	biological fluids	630:646	biological fluids	630:646	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	0	16	theme	biological	173:182	arg1	fluids					184:189	biological fluids	173:189	biological fluids prior to GC-FID analysis	173:214	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	8	17	theme	method	1624:1629	arg1	ability					1599:1605	the ability	1595:1605	the ability of the developed method to extract the target analytes from the biological samples	1595:1688	Finally, the ability of the developed method to extract the target analytes from the biological samples was evaluated.					
37178178	0	18	from	extraction	129:138	arg1	fluids					184:189	biological fluids	173:189	biological fluids prior to GC-FID analysis	173:214	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	0	19	theme	prior	191:195	arg1	fluids					184:189	biological fluids	173:189	biological fluids prior to GC-FID analysis	173:214	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	1	20	theme	novel	397:401	arg1	green					404:408	a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine	395:619	green	404:408	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	20	theme	novel	397:401	arg1	nanofibers					227:236	Composite nanofibers	217:236	Composite nanofibers	217:236	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	20	theme	novel	397:401	arg1	CLZ					622:624	CLZ	622:624	CLZ	622:624	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	2	21	theme	nanofibers	951:960	arg1	approved					966:973	approved	966:973	approved	966:973	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	21	theme	nanofibers	951:960	arg1	synthesis					928:936	the successful synthesis	913:936	the successful synthesis of composite nanofibers	913:960	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	8	22	theme	target	1646:1651	arg1	analytes					1653:1660	the target analytes	1642:1660	the target analytes	1642:1660	Finally, the ability of the developed method to extract the target analytes from the biological samples was evaluated.					
37178178	3	23	contain	have	1086:1089	arg2	efficiency					1107:1116	high extraction efficiency	1091:1116	high extraction efficiency	1091:1116	Due to the presence of β-cyclodextrins and CuO NPs rich of functional groups on their surface, the nanofibers have high extraction efficiency.					
37178178	3	23	contain	have	1086:1089	arg1	the nanofibers					1071:1084	the nanofibers	1071:1084	the nanofibers	1071:1084	Due to the presence of β-cyclodextrins and CuO NPs rich of functional groups on their surface, the nanofibers have high extraction efficiency.					
37178178	1	24	theme	copper	317:322	arg1	oxide					324:328	copper oxide	317:328	copper oxide	317:328	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	7	25	theme	sample	1558:1563	arg1	methods					1577:1583	other sample preparation methods	1552:1583	other sample preparation methods	1552:1583	In addition, excellent clean-up was achieved which is a great advantage over other sample preparation methods.					
37178178	8	26	theme	biological	1671:1680	arg1	samples					1682:1688	the biological samples	1667:1688	the biological samples	1667:1688	Finally, the ability of the developed method to extract the target analytes from the biological samples was evaluated.					
37178178	1	27	theme	simultaneous	509:520	arg1	extraction					522:531	the simultaneous extraction	505:531	the simultaneous extraction of three antidepressants drugs	505:562	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	3	28	theme	CuO	1019:1021	arg1	NPs					1023:1025	β-cyclodextrins and CuO NPs	999:1025	β-cyclodextrins and CuO NPs rich of functional groups on their surface	999:1068	Due to the presence of β-cyclodextrins and CuO NPs rich of functional groups on their surface, the nanofibers have high extraction efficiency.					
37178178	7	29	theme	great	1531:1535	arg1	advantage					1537:1545	a great advantage	1529:1545	a great advantage over other sample preparation methods	1529:1583	In addition, excellent clean-up was achieved which is a great advantage over other sample preparation methods.					
37178178	7	29	theme	great	1531:1535	arg1	which					1520:1524	which	1520:1524	which	1520:1524	In addition, excellent clean-up was achieved which is a great advantage over other sample preparation methods.					
37178178	0	30	theme	Polyvinyl	0:8	arg1	composite					51:59	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite	0:59	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite	0:59	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	3	31	theme	β-cyclodextrins	999:1013	arg1	NPs					1023:1025	β-cyclodextrins and CuO NPs	999:1025	β-cyclodextrins and CuO NPs rich of functional groups on their surface	999:1068	Due to the presence of β-cyclodextrins and CuO NPs rich of functional groups on their surface, the nanofibers have high extraction efficiency.					
37178178	2	32	theme	infrared	853:860	arg1	FT-IR					876:880	FT-IR	876:880	FT-IR	876:880	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	32	theme	infrared	853:860	arg1	spectroscopy					862:873	infrared spectroscopy	853:873	infrared spectroscopy (FT-IR)	853:881	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	1	33	theme	antidepressants	542:556	arg1	drugs					558:562	three antidepressants drugs	536:562	three antidepressants drugs	536:562	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	0	34	theme	acid/β-cyclodextrin/CuONP	25:49	arg1	composite					51:59	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite	0:59	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite	0:59	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	3	35	theme	functional	1035:1044	arg1	groups					1046:1051	functional groups	1035:1051	functional groups on their surface	1035:1068	Due to the presence of β-cyclodextrins and CuO NPs rich of functional groups on their surface, the nanofibers have high extraction efficiency.					
37178178	1	36	theme	β-cyclodextrin	290:303	arg1	nanofibers					227:236	Composite nanofibers	217:236	Composite nanofibers	217:236	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	36	theme	β-cyclodextrin	290:303	arg1	nanoparticles					330:342	citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles	272:342	citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs)	272:374	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	36	theme	β-cyclodextrin	290:303	arg1	NPs					371:373	PVA/CA/β-cyclodextrin/CuO NPs	345:373	PVA/CA/β-cyclodextrin/CuO NPs	345:373	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	3	37	theme	rich	1027:1030	arg1	NPs					1023:1025	β-cyclodextrins and CuO NPs	999:1025	β-cyclodextrins and CuO NPs rich of functional groups on their surface	999:1068	Due to the presence of β-cyclodextrins and CuO NPs rich of functional groups on their surface, the nanofibers have high extraction efficiency.					
37178178	1	38	theme	extraction	472:481	arg1	method					483:488	the pipette tip-micro-solid-phase extraction method	438:488	the pipette tip-micro-solid-phase extraction method (PT-µSPE)	438:498	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	38	theme	extraction	472:481	arg1	PT-µSPE					491:497	PT-µSPE	491:497	PT-µSPE	491:497	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	2	39	theme	electron	761:768	arg1	FE-SEM					782:787	FE-SEM	782:787	FE-SEM	782:787	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	39	theme	electron	761:768	arg1	microscopy					770:779	field emission scanning electron microscopy	737:779	field emission scanning electron microscopy (FE-SEM)	737:788	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	1	40	theme	polyvinyl	247:255	arg1	PVA					266:268	PVA	266:268	PVA	266:268	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	40	theme	polyvinyl	247:255	arg1	alcohol					257:263	polyvinyl alcohol	247:263	polyvinyl alcohol (PVA)	247:269	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	40	theme	polyvinyl	247:255	arg1	nanofibers					227:236	Composite nanofibers	217:236	Composite nanofibers	217:236	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	6	41	dep	%	1392:1392	arg1	n = 4					1407:1411	n = 4	1407:1411	n = 4	1407:1411	The relative standard deviation was 4.8 to 8.7% (within-day, n = 4) and 5.1 to 9.2% (between-day, n = 3) for 3 consecutive days.					
37178178	6	41	dep	%	1392:1392	arg1	within-day					1395:1404	within-day	1395:1404	within-day	1395:1404	The relative standard deviation was 4.8 to 8.7% (within-day, n = 4) and 5.1 to 9.2% (between-day, n = 3) for 3 consecutive days.					
37178178	2	42	theme	emission	743:750	arg1	FE-SEM					782:787	FE-SEM	782:787	FE-SEM	782:787	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	42	theme	emission	743:750	arg1	microscopy					770:779	field emission scanning electron microscopy	737:779	field emission scanning electron microscopy (FE-SEM)	737:788	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	43	theme	X-ray	809:813	arg1	EDX					829:831	EDX	829:831	EDX	829:831	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	43	theme	X-ray	809:813	arg1	spectroscopy					815:826	energy-dispersive X-ray spectroscopy	791:826	energy-dispersive X-ray spectroscopy (EDX)	791:832	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	3	44	theme	high	1091:1094	arg1	efficiency					1107:1116	high extraction efficiency	1091:1116	high extraction efficiency	1091:1116	Due to the presence of β-cyclodextrins and CuO NPs rich of functional groups on their surface, the nanofibers have high extraction efficiency.					
37178178	4	45	theme	determination	1241:1253	arg1	coefficient ≥ 0.99					1255:1272	a determination coefficient ≥ 0.99	1239:1272	a determination coefficient ≥ 0.99	1239:1272	Under the optimal conditions, the linear range for imipramine, citalopram, and clozapine was 0.1 to 1000.0 ng mL-1 with a determination coefficient ≥ 0.99.					
37178178	2	46	from	Fourier	835:841	arg1	results					724:730	the obtained results	711:730	the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD)	711:910	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	1	47	theme	gas	673:675	arg1	chromatography					677:690	gas chromatography	673:690	gas chromatography (GC-FID)	673:699	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	47	theme	gas	673:675	arg1	GC-FID					693:698	GC-FID	693:698	GC-FID	693:698	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	48	theme	citric	272:277	arg1	CA					285:286	CA	285:286	CA	285:286	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	48	theme	citric	272:277	arg1	acid					279:282	citric acid	272:282	citric acid (CA)	272:287	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	49	from	clozapine	611:619	arg1	fluids					641:646	biological fluids	630:646	biological fluids	630:646	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	2	50	dep	Fourier	835:841	arg1	transform					843:851	transform	843:851	transform infrared spectroscopy (FT-IR)	843:881	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	1	51	theme	efficient	415:423	arg1	IMP					583:585	IMP	583:585	IMP	583:585	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	51	theme	efficient	415:423	arg1	nanofibers					227:236	Composite nanofibers	217:236	Composite nanofibers	217:236	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	51	theme	efficient	415:423	arg1	namely imipramine					564:580	efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine	415:580	efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP)	415:586	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	6	52	dep	%	1428:1428	arg1	n = 3					1444:1448	n = 3	1444:1448	n = 3	1444:1448	The relative standard deviation was 4.8 to 8.7% (within-day, n = 4) and 5.1 to 9.2% (between-day, n = 3) for 3 consecutive days.					
37178178	6	52	dep	%	1428:1428	arg1	between-day					1431:1441	between-day	1431:1441	between-day	1431:1441	The relative standard deviation was 4.8 to 8.7% (within-day, n = 4) and 5.1 to 9.2% (between-day, n = 3) for 3 consecutive days.					
37178178	0	53	theme	antidepressant	149:162	arg1	drugs					164:168	three antidepressant drugs	143:168	three antidepressant drugs in biological fluids prior to GC-FID analysis	143:214	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	1	54	theme	pipette	442:448	arg1	method					483:488	the pipette tip-micro-solid-phase extraction method	438:488	the pipette tip-micro-solid-phase extraction method (PT-µSPE)	438:498	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	54	theme	pipette	442:448	arg1	PT-µSPE					491:497	PT-µSPE	491:497	PT-µSPE	491:497	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	4	55	dep	1000.0 ng mL-1	1219:1232	arg1	to					1216:1217	to	1216:1217	to	1216:1217	Under the optimal conditions, the linear range for imipramine, citalopram, and clozapine was 0.1 to 1000.0 ng mL-1 with a determination coefficient ≥ 0.99.					
37178178	2	56	theme	composite	941:949	arg1	nanofibers					951:960	composite nanofibers	941:960	composite nanofibers	941:960	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	57	theme	obtained	715:722	arg1	results					724:730	the obtained results	711:730	the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD)	711:910	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	1	58	from	namely imipramine	564:580	arg1	fluids					641:646	biological fluids	630:646	biological fluids	630:646	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	8	59	theme	developed	1614:1622	arg1	method					1624:1629	the developed method	1610:1629	the developed method	1610:1629	Finally, the ability of the developed method to extract the target analytes from the biological samples was evaluated.					
37178178	3	60	from	groups	1046:1051	arg1	surface					1062:1068	their surface	1056:1068	their surface	1056:1068	Due to the presence of β-cyclodextrins and CuO NPs rich of functional groups on their surface, the nanofibers have high extraction efficiency.					
37178178	0	61	theme	green	92:96	arg1	absorbent					98:106	an effective and green absorbent	75:106	an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis	75:214	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	2	62	theme	X-ray	888:892	arg1	diffraction					894:904	X-ray diffraction	888:904	X-ray diffraction (XRD)	888:910	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	62	theme	X-ray	888:892	arg1	XRD					907:909	XRD	907:909	XRD	907:909	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	4	63	theme	linear	1153:1158	arg1	range					1160:1164	the linear range	1149:1164	the linear range for imipramine, citalopram, and clozapine	1149:1206	Under the optimal conditions, the linear range for imipramine, citalopram, and clozapine was 0.1 to 1000.0 ng mL-1 with a determination coefficient ≥ 0.99.					
37178178	4	63	theme	linear	1153:1158	arg1	1000.0 ng mL-1					1219:1232	1000.0 ng mL-1	1219:1232	1000.0 ng mL-1	1219:1232	Under the optimal conditions, the linear range for imipramine, citalopram, and clozapine was 0.1 to 1000.0 ng mL-1 with a determination coefficient ≥ 0.99.					
37178178	8	64	from	samples	1682:1688	arg1	extract					1634:1640	extract	1634:1640	extract	1634:1640	Finally, the ability of the developed method to extract the target analytes from the biological samples was evaluated.					
37178178	0	65	theme	simultaneous	116:127	arg1	extraction					129:138	the simultaneous extraction	112:138	the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis	112:214	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	1	66	theme	oxide	324:328	arg1	nanofibers					227:236	Composite nanofibers	217:236	Composite nanofibers	217:236	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	66	theme	oxide	324:328	arg1	nanoparticles					330:342	citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles	272:342	citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs)	272:374	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	66	theme	oxide	324:328	arg1	NPs					371:373	PVA/CA/β-cyclodextrin/CuO NPs	345:373	PVA/CA/β-cyclodextrin/CuO NPs	345:373	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	7	67	theme	preparation	1565:1575	arg1	methods					1577:1583	other sample preparation methods	1552:1583	other sample preparation methods	1552:1583	In addition, excellent clean-up was achieved which is a great advantage over other sample preparation methods.					
37178178	7	68	theme	other	1552:1556	arg1	methods					1577:1583	other sample preparation methods	1552:1583	other sample preparation methods	1552:1583	In addition, excellent clean-up was achieved which is a great advantage over other sample preparation methods.					
37178178	4	69	theme	optimal	1129:1135	arg1	conditions					1137:1146	the optimal conditions	1125:1146	the optimal conditions	1125:1146	Under the optimal conditions, the linear range for imipramine, citalopram, and clozapine was 0.1 to 1000.0 ng mL-1 with a determination coefficient ≥ 0.99.					
37178178	0	70	from	drugs	164:168	arg1	fluids					184:189	biological fluids	173:189	biological fluids prior to GC-FID analysis	173:214	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	0	71	theme	alcohol/citric	10:23	arg1	composite					51:59	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite	0:59	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite	0:59	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	2	72	from	microscopy	770:779	arg1	results					724:730	the obtained results	711:730	the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD)	711:910	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	1	73	from	method	483:488	arg1	adsorbent					425:433	adsorbent	425:433	adsorbent	425:433	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	2	74	from	diffraction	894:904	arg1	results					724:730	the obtained results	711:730	the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD)	711:910	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	1	75	theme	drugs	558:562	arg1	extraction					522:531	the simultaneous extraction	505:531	the simultaneous extraction of three antidepressants drugs	505:562	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	3	76	theme	NPs	1023:1025	arg1	presence					987:994	the presence	983:994	the presence of β-cyclodextrins and CuO NPs rich of functional groups on their surface	983:1068	Due to the presence of β-cyclodextrins and CuO NPs rich of functional groups on their surface, the nanofibers have high extraction efficiency.					
37178178	1	77	theme	tip-micro-solid-phase	450:470	arg1	method					483:488	the pipette tip-micro-solid-phase extraction method	438:488	the pipette tip-micro-solid-phase extraction method (PT-µSPE)	438:498	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	77	theme	tip-micro-solid-phase	450:470	arg1	PT-µSPE					491:497	PT-µSPE	491:497	PT-µSPE	491:497	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	0	78	theme	effective	78:86	arg1	absorbent					98:106	an effective and green absorbent	75:106	an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis	75:214	Polyvinyl alcohol/citric acid/β-cyclodextrin/CuONP composite nanofibers as an effective and green absorbent for the simultaneous extraction of three antidepressant drugs in biological fluids prior to GC-FID analysis.					
37178178	3	79	theme	groups	1046:1051	arg1	rich					1027:1030	rich	1027:1030	rich	1027:1030	Due to the presence of β-cyclodextrins and CuO NPs rich of functional groups on their surface, the nanofibers have high extraction efficiency.					
37178178	1	80	from	adsorbent	425:433	arg1	method					483:488	the pipette tip-micro-solid-phase extraction method	438:488	the pipette tip-micro-solid-phase extraction method (PT-µSPE)	438:498	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	1	80	from	adsorbent	425:433	arg1	PT-µSPE					491:497	PT-µSPE	491:497	PT-µSPE	491:497	Composite nanofibers, namely, polyvinyl alcohol (PVA), citric acid (CA), β-cyclodextrin (β-CD), and copper oxide nanoparticles (PVA/CA/β-cyclodextrin/CuO NPs), were developed as a novel, green, and efficient adsorbent in the pipette tip-micro-solid-phase extraction method (PT-µSPE), for the simultaneous extraction of three antidepressants drugs namely imipramine (IMP), citalopram (CIT), and clozapine (CLZ) in biological fluids before quantification by gas chromatography (GC-FID).					
37178178	2	81	theme	scanning	752:759	arg1	FE-SEM					782:787	FE-SEM	782:787	FE-SEM	782:787	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	81	theme	scanning	752:759	arg1	microscopy					770:779	field emission scanning electron microscopy	737:779	field emission scanning electron microscopy (FE-SEM)	737:788	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	6	82	dep	8.7	1389:1391	arg1	to					1386:1387	to	1386:1387	to	1386:1387	The relative standard deviation was 4.8 to 8.7% (within-day, n = 4) and 5.1 to 9.2% (between-day, n = 3) for 3 consecutive days.					
37178178	2	83	theme	field	737:741	arg1	FE-SEM					782:787	FE-SEM	782:787	FE-SEM	782:787	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	83	theme	field	737:741	arg1	microscopy					770:779	field emission scanning electron microscopy	737:779	field emission scanning electron microscopy (FE-SEM)	737:788	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	84	theme	energy-dispersive	791:807	arg1	EDX					829:831	EDX	829:831	EDX	829:831	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	2	84	theme	energy-dispersive	791:807	arg1	spectroscopy					815:826	energy-dispersive X-ray spectroscopy	791:826	energy-dispersive X-ray spectroscopy (EDX)	791:832	Based on the obtained results from field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), the successful synthesis of composite nanofibers was approved.					
37178178	5	85	theme	detection	1289:1297	arg1	limits					1279:1284	The limits	1275:1284	The limits of detection (LODs)	1275:1304	The limits of detection (LODs) were in the range 0.03 to 0.15 ng mL-1.					
37178178	6	86	theme	standard	1359:1366	arg1	%					1392:1392	4.8 to 8.7%	1382:1392	4.8 to 8.7% (within-day, n = 4)	1382:1412	The relative standard deviation was 4.8 to 8.7% (within-day, n = 4) and 5.1 to 9.2% (between-day, n = 3) for 3 consecutive days.					
37178178	6	86	theme	standard	1359:1366	arg1	deviation					1368:1376	The relative standard deviation	1346:1376	The relative standard deviation	1346:1376	The relative standard deviation was 4.8 to 8.7% (within-day, n = 4) and 5.1 to 9.2% (between-day, n = 3) for 3 consecutive days.					
37355211	6	0	theme	scar	1010:1013	arg1	formation					1015:1023	glial scar formation	1004:1023	glial scar formation	1004:1023	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	2	1	theme	@	344:344	arg1	array					327:331	This epidural microneedle array	301:331	This epidural microneedle array	301:331	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	1	theme	@	344:344	arg1	MPSS					352:355	dubbed MNs@CD-MOF@MPSS	334:355	dubbed MNs@CD-MOF@MPSS	334:355	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	5	2	dep	in	738:739	arg1	vitro					741:745	vitro	741:745	vitro	741:745	In in vitro study, inward MNs increased cellular absorption of MPSS and then reduced LPS-induced M1 polarization of microglia.					
37355211	1	3	theme	metal-organic	267:279	arg1	CD-MOF					292:297	CD-MOF	292:297	CD-MOF	292:297	A new method of transdural delivering drugs to the spinal cord has been developed, involving the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	1	3	theme	metal-organic	267:279	arg1	framework					281:289	a β-cyclodextrin metal-organic framework	250:289	a β-cyclodextrin metal-organic framework (CD-MOF)	250:298	A new method of transdural delivering drugs to the spinal cord has been developed, involving the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	8	4	contain	has	1184:1186	arg2	potential					1188:1196	potential	1188:1196	potential for clinical application in spinal cord diseases	1188:1245	In conclusion, this new drug platform has potential for clinical application in spinal cord diseases and is a valuable composite for minimally transdural controlled drug delivery.					
37355211	8	4	contain	has	1184:1186	arg1	platform					1175:1182	this new drug platform	1161:1182	this new drug platform	1161:1182	In conclusion, this new drug platform has potential for clinical application in spinal cord diseases and is a valuable composite for minimally transdural controlled drug delivery.					
37355211	10	5	theme	epidural	1498:1505	arg1	patch					1519:1523	The epidural microneedle patch	1494:1523	The epidural microneedle patch	1494:1523	The epidural microneedle patch boasts high drug loading capacity, the ability to penetrate the dura, and controlled release.					
37355211	7	6	theme	cytokines	1122:1130	arg1	downregulation					1043:1056	the downregulation	1039:1056	the downregulation of the NLRP3-positive inflammasome and related pro-inflammatory cytokines	1039:1130	Furthermore, the downregulation of the NLRP3-positive inflammasome and related pro-inflammatory cytokines was observed.					
37355211	9	7	theme	microneedle	1391:1401	arg1	patch					1403:1407	a new epidural microneedle patch	1376:1407	a new epidural microneedle patch made up of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF)	1376:1491	STATEMENT OF SIGNIFICANCE: This research presents a new epidural microneedle patch made up of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	10	8	theme	loading	1542:1548	arg1	capacity					1550:1557	high drug loading capacity	1532:1557	high drug loading capacity	1532:1557	The epidural microneedle patch boasts high drug loading capacity, the ability to penetrate the dura, and controlled release.					
37355211	2	9	theme	epidural	306:313	arg1	array					327:331	This epidural microneedle array	301:331	This epidural microneedle array	301:331	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	9	theme	epidural	306:313	arg1	MPSS					352:355	dubbed MNs@CD-MOF@MPSS	334:355	dubbed MNs@CD-MOF@MPSS	334:355	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	8	10	theme	clinical	1202:1209	arg1	application					1211:1221	clinical application	1202:1221	clinical application	1202:1221	In conclusion, this new drug platform has potential for clinical application in spinal cord diseases and is a valuable composite for minimally transdural controlled drug delivery.					
37355211	10	11	theme	high	1532:1535	arg1	capacity					1550:1557	high drug loading capacity	1532:1557	high drug loading capacity	1532:1557	The epidural microneedle patch boasts high drug loading capacity, the ability to penetrate the dura, and controlled release.					
37355211	11	12	theme	neurological	1729:1740	arg1	function					1742:1749	neurological function	1729:1749	neurological function	1729:1749	When loaded with methylprednisolone sodium succinate (MPSS), it effectively reduces inflammation and improves neurological function after spinal cord injury.					
37355211	8	13	dep	transdural	1289:1298	arg1	controlled					1300:1309	controlled	1300:1309	controlled	1300:1309	In conclusion, this new drug platform has potential for clinical application in spinal cord diseases and is a valuable composite for minimally transdural controlled drug delivery.					
37355211	8	14	from	potential	1188:1196	arg1	diseases					1238:1245	spinal cord diseases	1226:1245	spinal cord diseases	1226:1245	In conclusion, this new drug platform has potential for clinical application in spinal cord diseases and is a valuable composite for minimally transdural controlled drug delivery.					
37355211	5	15	theme	cellular	775:782	arg1	absorption					784:793	cellular absorption	775:793	cellular absorption of MPSS	775:801	In in vitro study, inward MNs increased cellular absorption of MPSS and then reduced LPS-induced M1 polarization of microglia.					
37355211	8	16	theme	new	1166:1168	arg1	platform					1175:1182	this new drug platform	1161:1182	this new drug platform	1161:1182	In conclusion, this new drug platform has potential for clinical application in spinal cord diseases and is a valuable composite for minimally transdural controlled drug delivery.					
37355211	8	17	theme	cord	1233:1236	arg1	diseases					1238:1245	spinal cord diseases	1226:1245	spinal cord diseases	1226:1245	In conclusion, this new drug platform has potential for clinical application in spinal cord diseases and is a valuable composite for minimally transdural controlled drug delivery.					
37355211	11	18	theme	cord	1764:1767	arg1	injury					1769:1774	spinal cord injury	1757:1774	spinal cord injury	1757:1774	When loaded with methylprednisolone sodium succinate (MPSS), it effectively reduces inflammation and improves neurological function after spinal cord injury.					
37355211	1	19	theme	transdural	139:148	arg1	drugs					161:165	transdural delivering drugs	139:165	transdural delivering drugs	139:165	A new method of transdural delivering drugs to the spinal cord has been developed, involving the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	1	20	theme	drugs	161:165	arg1	method					129:134	A new method	123:134	A new method of transdural delivering drugs to the spinal cord	123:184	A new method of transdural delivering drugs to the spinal cord has been developed, involving the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	6	21	theme	BMS	946:948	arg1	scores					950:955	improved BMS scores	937:955	improved BMS scores	937:955	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	3	22	from	micropores	515:524	arg1	dura					533:536	the dura	529:536	the dura	529:536	MNs allows to generate micropores in the dura for direct drug delivery to the spinal cord, overcoming tissue barriers and targeting damaged regions.					
37355211	3	23	theme	drug	549:552	arg1	delivery					554:561	direct drug delivery	542:561	direct drug delivery to the spinal cord	542:580	MNs allows to generate micropores in the dura for direct drug delivery to the spinal cord, overcoming tissue barriers and targeting damaged regions.					
37355211	4	24	from	MNs	730:732	arg1	release					696:702	a secondary extended release	675:702	a secondary extended release after separating from the MNs	675:732	Additionally, the CD-MOF provides a secondary extended release after separating from the MNs.					
37355211	9	25	theme	metal-organic	1460:1472	arg1	CD-MOF					1485:1490	CD-MOF	1485:1490	CD-MOF	1485:1490	STATEMENT OF SIGNIFICANCE: This research presents a new epidural microneedle patch made up of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	9	25	theme	metal-organic	1460:1472	arg1	framework					1474:1482	a β-cyclodextrin metal-organic framework	1443:1482	a β-cyclodextrin metal-organic framework (CD-MOF)	1443:1491	STATEMENT OF SIGNIFICANCE: This research presents a new epidural microneedle patch made up of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	2	26	theme	cord	450:453	arg1	injury					455:460	spinal cord injury	443:460	spinal cord injury (SCI)	443:466	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	26	theme	cord	450:453	arg1	SCI					463:465	SCI	463:465	SCI	463:465	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	27	used	utilized	365:372	arg2	MPSS					352:355	dubbed MNs@CD-MOF@MPSS	334:355	dubbed MNs@CD-MOF@MPSS	334:355	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	27	used	utilized	365:372	arg2	array					327:331	This epidural microneedle array	301:331	This epidural microneedle array	301:331	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	0	28	theme	cord	110:113	arg1	injury					115:120	spinal cord injury	103:120	spinal cord injury	103:120	Microneedle/CD-MOF-mediated transdural controlled release of methylprednisolone sodium succinate after spinal cord injury.					
37355211	5	29	theme	M1	832:833	arg1	polarization					835:846	LPS-induced M1 polarization	820:846	LPS-induced M1 polarization of microglia	820:859	In in vitro study, inward MNs increased cellular absorption of MPSS and then reduced LPS-induced M1 polarization of microglia.					
37355211	6	30	theme	delivery	917:924	arg1	method					902:907	this method	897:907	this method of drug delivery	897:924	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	6	31	from	scores	950:955	arg1	formation					1015:1023	glial scar formation	1004:1023	glial scar formation	1004:1023	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	6	31	from	scores	950:955	arg1	microphage					989:998	M1 phenotype microphage	976:998	M1 phenotype microphage	976:998	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	12	32	theme	cord	1858:1861	arg1	diseases					1863:1870	spinal cord diseases	1851:1870	spinal cord diseases	1851:1870	Therefore, it is a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting.					
37355211	9	33	theme	new	1378:1380	arg1	patch					1403:1407	a new epidural microneedle patch	1376:1407	a new epidural microneedle patch made up of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF)	1376:1491	STATEMENT OF SIGNIFICANCE: This research presents a new epidural microneedle patch made up of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	0	34	theme	methylprednisolone	61:78	arg1	succinate					87:95	methylprednisolone sodium succinate	61:95	methylprednisolone sodium succinate	61:95	Microneedle/CD-MOF-mediated transdural controlled release of methylprednisolone sodium succinate after spinal cord injury.					
37355211	0	35	theme	Microneedle/CD-MOF-mediated	0:26	arg1	transdural					28:37	Microneedle/CD-MOF-mediated transdural	0:37	Microneedle/CD-MOF-mediated transdural	0:37	Microneedle/CD-MOF-mediated transdural controlled release of methylprednisolone sodium succinate after spinal cord injury.					
37355211	1	36	theme	framework	281:289	arg1	use					220:222	the use	216:222	the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF)	216:298	A new method of transdural delivering drugs to the spinal cord has been developed, involving the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	6	37	theme	M1	976:977	arg1	microphage					989:998	M1 phenotype microphage	976:998	M1 phenotype microphage	976:998	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	4	38	theme	extended	687:694	arg1	release					696:702	a secondary extended release	675:702	a secondary extended release after separating from the MNs	675:732	Additionally, the CD-MOF provides a secondary extended release after separating from the MNs.					
37355211	2	39	theme	sodium	404:409	arg1	MPSS					422:425	MPSS	422:425	MPSS	422:425	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	39	theme	sodium	404:409	arg1	succinate					411:419	methylprednisolone sodium succinate	385:419	methylprednisolone sodium succinate (MPSS)	385:426	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	7	40	theme	related	1097:1103	arg1	cytokines					1122:1130	related pro-inflammatory cytokines	1097:1130	related pro-inflammatory cytokines	1097:1130	Furthermore, the downregulation of the NLRP3-positive inflammasome and related pro-inflammatory cytokines was observed.					
37355211	12	41	theme	novel	1796:1800	arg1	platform					1821:1828	a novel and promising drug platform	1794:1828	a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting	1794:1892	Therefore, it is a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting.					
37355211	12	41	theme	novel	1796:1800	arg1	it					1788:1789	it	1788:1789	it	1788:1789	Therefore, it is a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting.					
37355211	11	42	theme	methylprednisolone	1636:1653	arg1	succinate					1662:1670	methylprednisolone sodium succinate	1636:1670	methylprednisolone sodium succinate (MPSS)	1636:1677	When loaded with methylprednisolone sodium succinate (MPSS), it effectively reduces inflammation and improves neurological function after spinal cord injury.					
37355211	11	42	theme	methylprednisolone	1636:1653	arg1	MPSS					1673:1676	MPSS	1673:1676	MPSS	1673:1676	When loaded with methylprednisolone sodium succinate (MPSS), it effectively reduces inflammation and improves neurological function after spinal cord injury.					
37355211	10	43	theme	controlled	1599:1608	arg1	release					1610:1616	controlled release	1599:1616	controlled release	1599:1616	The epidural microneedle patch boasts high drug loading capacity, the ability to penetrate the dura, and controlled release.					
37355211	7	44	theme	inflammasome	1080:1091	arg1	downregulation					1043:1056	the downregulation	1039:1056	the downregulation of the NLRP3-positive inflammasome and related pro-inflammatory cytokines	1039:1130	Furthermore, the downregulation of the NLRP3-positive inflammasome and related pro-inflammatory cytokines was observed.					
37355211	12	45	theme	promising	1806:1814	arg1	platform					1821:1828	a novel and promising drug platform	1794:1828	a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting	1794:1892	Therefore, it is a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting.					
37355211	12	45	theme	promising	1806:1814	arg1	it					1788:1789	it	1788:1789	it	1788:1789	Therefore, it is a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting.					
37355211	3	46	theme	spinal	570:575	arg1	cord					577:580	the spinal cord	566:580	the spinal cord	566:580	MNs allows to generate micropores in the dura for direct drug delivery to the spinal cord, overcoming tissue barriers and targeting damaged regions.					
37355211	2	47	theme	MNs	341:343	arg1	array					327:331	This epidural microneedle array	301:331	This epidural microneedle array	301:331	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	47	theme	MNs	341:343	arg1	MPSS					352:355	dubbed MNs@CD-MOF@MPSS	334:355	dubbed MNs@CD-MOF@MPSS	334:355	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	0	48	theme	sodium	80:85	arg1	succinate					87:95	methylprednisolone sodium succinate	61:95	methylprednisolone sodium succinate	61:95	Microneedle/CD-MOF-mediated transdural controlled release of methylprednisolone sodium succinate after spinal cord injury.					
37355211	1	49	theme	microneedles	227:238	arg1	use					220:222	the use	216:222	the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF)	216:298	A new method of transdural delivering drugs to the spinal cord has been developed, involving the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	3	50	theme	tissue	594:599	arg1	barriers					601:608	tissue barriers	594:608	tissue barriers	594:608	MNs allows to generate micropores in the dura for direct drug delivery to the spinal cord, overcoming tissue barriers and targeting damaged regions.					
37355211	2	51	theme	CD-MOF	345:350	arg1	array					327:331	This epidural microneedle array	301:331	This epidural microneedle array	301:331	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	51	theme	CD-MOF	345:350	arg1	MPSS					352:355	dubbed MNs@CD-MOF@MPSS	334:355	dubbed MNs@CD-MOF@MPSS	334:355	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	6	52	theme	glial	1004:1008	arg1	formation					1015:1023	glial scar formation	1004:1023	glial scar formation	1004:1023	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	3	53	theme	damaged	624:630	arg1	regions					632:638	damaged regions	624:638	damaged regions	624:638	MNs allows to generate micropores in the dura for direct drug delivery to the spinal cord, overcoming tissue barriers and targeting damaged regions.					
37355211	1	54	theme	β-cyclodextrin	252:265	arg1	CD-MOF					292:297	CD-MOF	292:297	CD-MOF	292:297	A new method of transdural delivering drugs to the spinal cord has been developed, involving the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	1	54	theme	β-cyclodextrin	252:265	arg1	framework					281:289	a β-cyclodextrin metal-organic framework	250:289	a β-cyclodextrin metal-organic framework (CD-MOF)	250:298	A new method of transdural delivering drugs to the spinal cord has been developed, involving the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	5	55	theme	LPS-induced	820:830	arg1	polarization					835:846	LPS-induced M1 polarization	820:846	LPS-induced M1 polarization of microglia	820:859	In in vitro study, inward MNs increased cellular absorption of MPSS and then reduced LPS-induced M1 polarization of microglia.					
37355211	10	56	theme	microneedle	1507:1517	arg1	patch					1519:1523	The epidural microneedle patch	1494:1523	The epidural microneedle patch	1494:1523	The epidural microneedle patch boasts high drug loading capacity, the ability to penetrate the dura, and controlled release.					
37355211	9	57	theme	epidural	1382:1389	arg1	patch					1403:1407	a new epidural microneedle patch	1376:1407	a new epidural microneedle patch made up of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF)	1376:1491	STATEMENT OF SIGNIFICANCE: This research presents a new epidural microneedle patch made up of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	8	58	theme	drug	1170:1173	arg1	platform					1175:1182	this new drug platform	1161:1182	this new drug platform	1161:1182	In conclusion, this new drug platform has potential for clinical application in spinal cord diseases and is a valuable composite for minimally transdural controlled drug delivery.					
37355211	2	59	theme	microneedle	315:325	arg1	array					327:331	This epidural microneedle array	301:331	This epidural microneedle array	301:331	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	59	theme	microneedle	315:325	arg1	MPSS					352:355	dubbed MNs@CD-MOF@MPSS	334:355	dubbed MNs@CD-MOF@MPSS	334:355	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	5	60	theme	MPSS	798:801	arg1	absorption					784:793	cellular absorption	775:793	cellular absorption of MPSS	775:801	In in vitro study, inward MNs increased cellular absorption of MPSS and then reduced LPS-induced M1 polarization of microglia.					
37355211	10	61	theme	drug	1537:1540	arg1	capacity					1550:1557	high drug loading capacity	1532:1557	high drug loading capacity	1532:1557	The epidural microneedle patch boasts high drug loading capacity, the ability to penetrate the dura, and controlled release.					
37355211	11	62	theme	spinal	1757:1762	arg1	injury					1769:1774	spinal cord injury	1757:1774	spinal cord injury	1757:1774	When loaded with methylprednisolone sodium succinate (MPSS), it effectively reduces inflammation and improves neurological function after spinal cord injury.					
37355211	6	63	from	reduction	963:971	arg1	formation					1015:1023	glial scar formation	1004:1023	glial scar formation	1004:1023	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	6	63	from	reduction	963:971	arg1	microphage					989:998	M1 phenotype microphage	976:998	M1 phenotype microphage	976:998	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	5	64	theme	inward	754:759	arg1	MNs					761:763	inward MNs	754:763	inward MNs	754:763	In in vitro study, inward MNs increased cellular absorption of MPSS and then reduced LPS-induced M1 polarization of microglia.					
37355211	2	65	theme	controlled	473:482	arg1	manner					484:489	a controlled manner	471:489	a controlled manner	471:489	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	12	66	theme	clinical	1877:1884	arg1	setting					1886:1892	a clinical setting	1875:1892	a clinical setting	1875:1892	Therefore, it is a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting.					
37355211	6	67	theme	animal	866:871	arg1	studies					873:879	And animal studies	862:879	studies	873:879	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	8	68	theme	spinal	1226:1231	arg1	diseases					1238:1245	spinal cord diseases	1226:1245	spinal cord diseases	1226:1245	In conclusion, this new drug platform has potential for clinical application in spinal cord diseases and is a valuable composite for minimally transdural controlled drug delivery.					
37355211	1	69	theme	delivering	150:159	arg1	drugs					161:165	transdural delivering drugs	139:165	transdural delivering drugs	139:165	A new method of transdural delivering drugs to the spinal cord has been developed, involving the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	0	70	theme	spinal	103:108	arg1	injury					115:120	spinal cord injury	103:120	spinal cord injury	103:120	Microneedle/CD-MOF-mediated transdural controlled release of methylprednisolone sodium succinate after spinal cord injury.					
37355211	3	71	theme	direct	542:547	arg1	delivery					554:561	direct drug delivery	542:561	direct drug delivery to the spinal cord	542:580	MNs allows to generate micropores in the dura for direct drug delivery to the spinal cord, overcoming tissue barriers and targeting damaged regions.					
37355211	9	72	theme	β-cyclodextrin	1445:1458	arg1	CD-MOF					1485:1490	CD-MOF	1485:1490	CD-MOF	1485:1490	STATEMENT OF SIGNIFICANCE: This research presents a new epidural microneedle patch made up of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	9	72	theme	β-cyclodextrin	1445:1458	arg1	framework					1474:1482	a β-cyclodextrin metal-organic framework	1443:1482	a β-cyclodextrin metal-organic framework (CD-MOF)	1443:1491	STATEMENT OF SIGNIFICANCE: This research presents a new epidural microneedle patch made up of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	6	73	theme	improved	937:944	arg1	scores					950:955	improved BMS scores	937:955	improved BMS scores	937:955	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	2	74	theme	injury	455:460	arg1	injury					455:460	spinal cord injury	443:460	spinal cord injury (SCI)	443:466	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	74	theme	injury	455:460	arg1	site					435:438	the site	431:438	the site of spinal cord injury (SCI)	431:466	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	12	75	theme	spinal	1851:1856	arg1	diseases					1863:1870	spinal cord diseases	1851:1870	spinal cord diseases	1851:1870	Therefore, it is a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting.					
37355211	1	76	theme	spinal	174:179	arg1	cord					181:184	the spinal cord	170:184	the spinal cord	170:184	A new method of transdural delivering drugs to the spinal cord has been developed, involving the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	12	77	from	treatment	1838:1846	arg1	setting					1886:1892	a clinical setting	1875:1892	a clinical setting	1875:1892	Therefore, it is a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting.					
37355211	2	78	theme	spinal	443:448	arg1	injury					455:460	spinal cord injury	443:460	spinal cord injury (SCI)	443:466	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	78	theme	spinal	443:448	arg1	SCI					463:465	SCI	463:465	SCI	463:465	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	8	79	theme	valuable	1256:1263	arg1	composite					1265:1273	a valuable composite	1254:1273	a valuable composite for minimally transdural controlled drug delivery	1254:1323	In conclusion, this new drug platform has potential for clinical application in spinal cord diseases and is a valuable composite for minimally transdural controlled drug delivery.					
37355211	12	80	theme	diseases	1863:1870	arg1	treatment					1838:1846	the treatment	1834:1846	the treatment of spinal cord diseases in a clinical setting	1834:1892	Therefore, it is a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting.					
37355211	6	81	theme	drug	912:915	arg1	delivery					917:924	drug delivery	912:924	drug delivery	912:924	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	5	82	theme	microglia	851:859	arg1	polarization					835:846	LPS-induced M1 polarization	820:846	LPS-induced M1 polarization of microglia	820:859	In in vitro study, inward MNs increased cellular absorption of MPSS and then reduced LPS-induced M1 polarization of microglia.					
37355211	8	83	theme	transdural	1289:1298	arg1	delivery					1316:1323	minimally transdural controlled drug delivery	1279:1323	minimally transdural controlled drug delivery	1279:1323	In conclusion, this new drug platform has potential for clinical application in spinal cord diseases and is a valuable composite for minimally transdural controlled drug delivery.					
37355211	5	84	theme	in	738:739	arg1	study					747:751	in vitro study	738:751	in vitro study	738:751	In in vitro study, inward MNs increased cellular absorption of MPSS and then reduced LPS-induced M1 polarization of microglia.					
37355211	6	85	theme	phenotype	979:987	arg1	microphage					989:998	M1 phenotype microphage	976:998	M1 phenotype microphage	976:998	And animal studies have shown that this method of drug delivery results in improved BMS scores and a reduction in M1 phenotype microphage and glial scar formation.					
37355211	8	86	theme	drug	1311:1314	arg1	delivery					1316:1323	minimally transdural controlled drug delivery	1279:1323	minimally transdural controlled drug delivery	1279:1323	In conclusion, this new drug platform has potential for clinical application in spinal cord diseases and is a valuable composite for minimally transdural controlled drug delivery.					
37355211	1	87	theme	new	125:127	arg1	method					129:134	A new method	123:134	A new method of transdural delivering drugs to the spinal cord	123:184	A new method of transdural delivering drugs to the spinal cord has been developed, involving the use of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	4	88	theme	secondary	677:685	arg1	release					696:702	a secondary extended release	675:702	a secondary extended release after separating from the MNs	675:732	Additionally, the CD-MOF provides a secondary extended release after separating from the MNs.					
37355211	2	89	theme	methylprednisolone	385:402	arg1	MPSS					422:425	MPSS	422:425	MPSS	422:425	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	89	theme	methylprednisolone	385:402	arg1	succinate					411:419	methylprednisolone sodium succinate	385:419	methylprednisolone sodium succinate (MPSS)	385:426	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	7	90	theme	NLRP3-positive	1065:1078	arg1	inflammasome					1080:1091	the NLRP3-positive inflammasome	1061:1091	the NLRP3-positive inflammasome	1061:1091	Furthermore, the downregulation of the NLRP3-positive inflammasome and related pro-inflammatory cytokines was observed.					
37355211	12	91	theme	drug	1816:1819	arg1	platform					1821:1828	a novel and promising drug platform	1794:1828	a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting	1794:1892	Therefore, it is a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting.					
37355211	12	91	theme	drug	1816:1819	arg1	it					1788:1789	it	1788:1789	it	1788:1789	Therefore, it is a novel and promising drug platform for the treatment of spinal cord diseases in a clinical setting.					
37355211	0	92	theme	succinate	87:95	arg1	release					50:56	release	50:56	release of methylprednisolone sodium succinate after spinal cord injury	50:120	Microneedle/CD-MOF-mediated transdural controlled release of methylprednisolone sodium succinate after spinal cord injury.					
37355211	2	93	theme	dubbed	334:339	arg1	array					327:331	This epidural microneedle array	301:331	This epidural microneedle array	301:331	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	93	theme	dubbed	334:339	arg1	MPSS					352:355	dubbed MNs@CD-MOF@MPSS	334:355	dubbed MNs@CD-MOF@MPSS	334:355	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	11	94	theme	sodium	1655:1660	arg1	succinate					1662:1670	methylprednisolone sodium succinate	1636:1670	methylprednisolone sodium succinate (MPSS)	1636:1677	When loaded with methylprednisolone sodium succinate (MPSS), it effectively reduces inflammation and improves neurological function after spinal cord injury.					
37355211	11	94	theme	sodium	1655:1660	arg1	MPSS					1673:1676	MPSS	1673:1676	MPSS	1673:1676	When loaded with methylprednisolone sodium succinate (MPSS), it effectively reduces inflammation and improves neurological function after spinal cord injury.					
37355211	2	95	theme	@	351:351	arg1	array					327:331	This epidural microneedle array	301:331	This epidural microneedle array	301:331	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	2	95	theme	@	351:351	arg1	MPSS					352:355	dubbed MNs@CD-MOF@MPSS	334:355	dubbed MNs@CD-MOF@MPSS	334:355	This epidural microneedle array, dubbed MNs@CD-MOF@MPSS, can be utilized to deliver methylprednisolone sodium succinate (MPSS) to the site of spinal cord injury (SCI) in a controlled manner.					
37355211	9	96	theme	SIGNIFICANCE	1339:1350	arg1	STATEMENT					1326:1334	STATEMENT	1326:1334	STATEMENT OF SIGNIFICANCE	1326:1350	STATEMENT OF SIGNIFICANCE: This research presents a new epidural microneedle patch made up of microneedles (MNs) and a β-cyclodextrin metal-organic framework (CD-MOF).					
37355211	7	97	theme	pro-inflammatory	1105:1120	arg1	cytokines					1122:1130	related pro-inflammatory cytokines	1097:1130	related pro-inflammatory cytokines	1097:1130	Furthermore, the downregulation of the NLRP3-positive inflammasome and related pro-inflammatory cytokines was observed.					
35917787	5	0	theme	hydrophilic	748:758	arg1	WBC					760:762	the hydrophilic WBC	744:762	the hydrophilic WBC	744:762	The nanocomplexes had a typical core-shell structure according to TEM characterization, where proteins aggregate to form a hydrophobic core and the hydrophilic WBC and STP crosslinked to form the shell.					
35917787	5	1	theme	typical	624:630	arg1	structure					643:651	a typical core-shell structure	622:651	a typical core-shell structure	622:651	The nanocomplexes had a typical core-shell structure according to TEM characterization, where proteins aggregate to form a hydrophobic core and the hydrophilic WBC and STP crosslinked to form the shell.					
35917787	2	2	theme	sodium	323:328	arg1	STP					348:350	STP	348:350	STP	348:350	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	2	2	theme	sodium	323:328	arg1	tripolyphosphate					330:345	sodium tripolyphosphate	323:345	sodium tripolyphosphate (STP)	323:351	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	5	3	theme	core-shell	632:641	arg1	structure					643:651	a typical core-shell structure	622:651	a typical core-shell structure	622:651	The nanocomplexes had a typical core-shell structure according to TEM characterization, where proteins aggregate to form a hydrophobic core and the hydrophilic WBC and STP crosslinked to form the shell.					
35917787	7	4	theme	simulated	946:954	arg1	experiments					983:993	In vitro simulated gastrointestinal digestion experiments	937:993	In vitro simulated gastrointestinal digestion experiments	937:993	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	3	5	theme	relative	483:490	arg1	state					502:506	a relative unfolding state	481:506	a relative unfolding state	481:506	Fluorescence spectroscopy and zeta-potential evidenced that the embedding of WBC into the WPs favored the formation composites a relative unfolding state.					
35917787	5	6	contain	had	618:620	arg2	structure					643:651	a typical core-shell structure	622:651	a typical core-shell structure	622:651	The nanocomplexes had a typical core-shell structure according to TEM characterization, where proteins aggregate to form a hydrophobic core and the hydrophilic WBC and STP crosslinked to form the shell.					
35917787	5	6	contain	had	618:620	arg1	nanocomplexes					604:616	The nanocomplexes	600:616	The nanocomplexes	600:616	The nanocomplexes had a typical core-shell structure according to TEM characterization, where proteins aggregate to form a hydrophobic core and the hydrophilic WBC and STP crosslinked to form the shell.					
35917787	7	7	theme	gastrointestinal	956:971	arg1	experiments					983:993	In vitro simulated gastrointestinal digestion experiments	937:993	In vitro simulated gastrointestinal digestion experiments	937:993	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	6	8	theme	WWBCs	870:874	arg1	composites					876:885	WWBCs composites	870:885	WWBCs composites	870:885	To improve the bioavailability of curcumin, it was encapsulated in WWBCs composites by participating in their structural co-assembly.					
35917787	7	9	theme	In	937:938	arg1	experiments					983:993	In vitro simulated gastrointestinal digestion experiments	937:993	In vitro simulated gastrointestinal digestion experiments	937:993	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	7	10	contain	possessed	1042:1050	arg2	function					1097:1104	gastrointestinal slow and controlled release function	1052:1104	gastrointestinal slow and controlled release function	1052:1104	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	7	10	contain	possessed	1042:1050	arg1	curcumin					1011:1018	the curcumin	1007:1018	the curcumin encapsulated in WWBCs	1007:1040	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	5	11	theme	hydrophobic	723:733	arg1	core					735:738	a hydrophobic core	721:738	a hydrophobic core	721:738	The nanocomplexes had a typical core-shell structure according to TEM characterization, where proteins aggregate to form a hydrophobic core and the hydrophilic WBC and STP crosslinked to form the shell.					
35917787	3	12	dep	formation	460:468	arg1	composites					470:479	composites	470:479	composites	470:479	Fluorescence spectroscopy and zeta-potential evidenced that the embedding of WBC into the WPs favored the formation composites a relative unfolding state.					
35917787	0	13	theme	curcumin	107:114	arg1	bioavailability					88:102	the bioavailability	84:102	the bioavailability of curcumin	84:114	Hydrophilic co-assembly of wheat gluten proteins and wheat bran cellulose improving the bioavailability of curcumin.					
35917787	7	14	theme	final	1114:1118	arg1	release					1120:1126	a final release	1112:1126	a final release of curcumin of 77.8 ± 2.3 %	1112:1154	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	0	15	theme	Hydrophilic	0:10	arg1	co-assembly					12:22	Hydrophilic co-assembly	0:22	Hydrophilic co-assembly of wheat gluten proteins	0:47	Hydrophilic co-assembly of wheat gluten proteins and wheat bran cellulose improving the bioavailability of curcumin.					
35917787	1	16	theme	effective	176:184	arg1	system					195:200	an effective delivery system	173:200	an effective delivery system for curcumin	173:213	Low-cost wheat by-products have been modified to become an effective delivery system for curcumin.					
35917787	4	17	with	nanocomposite	523:535	arg1	STP					542:544	STP	542:544	STP	542:544	Modifying the nanocomposite with STP lowered the Dh and PDI of the co-assembled structure.					
35917787	7	18	theme	slow	1069:1072	arg1	function					1097:1104	gastrointestinal slow and controlled release function	1052:1104	gastrointestinal slow and controlled release function	1052:1104	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	1	19	theme	delivery	186:193	arg1	system					195:200	an effective delivery system	173:200	an effective delivery system for curcumin	173:213	Low-cost wheat by-products have been modified to become an effective delivery system for curcumin.					
35917787	3	20	theme	Fluorescence	354:365	arg1	spectroscopy					367:378	Fluorescence spectroscopy	354:378	Fluorescence spectroscopy	354:378	Fluorescence spectroscopy and zeta-potential evidenced that the embedding of WBC into the WPs favored the formation composites a relative unfolding state.					
35917787	0	21	theme	wheat	27:31	arg1	proteins					40:47	wheat gluten proteins	27:47	wheat gluten proteins	27:47	Hydrophilic co-assembly of wheat gluten proteins and wheat bran cellulose improving the bioavailability of curcumin.					
35917787	7	22	theme	release	1089:1095	arg1	function					1097:1104	gastrointestinal slow and controlled release function	1052:1104	gastrointestinal slow and controlled release function	1052:1104	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	0	23	theme	proteins	40:47	arg1	bran					59:62	wheat bran	53:62	wheat bran	53:62	Hydrophilic co-assembly of wheat gluten proteins and wheat bran cellulose improving the bioavailability of curcumin.					
35917787	0	23	theme	proteins	40:47	arg1	co-assembly					12:22	Hydrophilic co-assembly	0:22	Hydrophilic co-assembly of wheat gluten proteins	0:47	Hydrophilic co-assembly of wheat gluten proteins and wheat bran cellulose improving the bioavailability of curcumin.					
35917787	3	24	theme	WBC	431:433	arg1	embedding					418:426	the embedding	414:426	the embedding of WBC into the WPs	414:446	Fluorescence spectroscopy and zeta-potential evidenced that the embedding of WBC into the WPs favored the formation composites a relative unfolding state.					
35917787	0	25	theme	gluten	33:38	arg1	proteins					40:47	wheat gluten proteins	27:47	wheat gluten proteins	27:47	Hydrophilic co-assembly of wheat gluten proteins and wheat bran cellulose improving the bioavailability of curcumin.					
35917787	4	26	theme	Modifying	509:517	arg1	nanocomposite					523:535	the nanocomposite	519:535	Modifying the nanocomposite with STP	509:544	Modifying the nanocomposite with STP lowered the Dh and PDI of the co-assembled structure.					
35917787	7	27	theme	controlled	1078:1087	arg1	function					1097:1104	gastrointestinal slow and controlled release function	1052:1104	gastrointestinal slow and controlled release function	1052:1104	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	6	28	theme	curcumin	837:844	arg1	bioavailability					818:832	the bioavailability	814:832	the bioavailability of curcumin	814:844	To improve the bioavailability of curcumin, it was encapsulated in WWBCs composites by participating in their structural co-assembly.					
35917787	0	29	theme	wheat	53:57	arg1	bran					59:62	wheat bran	53:62	wheat bran	53:62	Hydrophilic co-assembly of wheat gluten proteins and wheat bran cellulose improving the bioavailability of curcumin.					
35917787	7	30	theme	gastrointestinal	1052:1067	arg1	function					1097:1104	gastrointestinal slow and controlled release function	1052:1104	gastrointestinal slow and controlled release function	1052:1104	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	4	31	dep	Dh	558:559	arg1	the					554:556	the	554:556	the	554:556	Modifying the nanocomposite with STP lowered the Dh and PDI of the co-assembled structure.					
35917787	2	32	theme	cellulose	227:235	arg1	WPs					270:272	WPs	270:272	WPs	270:272	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	2	32	theme	cellulose	227:235	arg1	proteins					260:267	Wheat bran cellulose (WBC) and wheat gluten proteins	216:267	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs)	216:273	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	1	33	theme	Low-cost	117:124	arg1	by-products					132:142	Low-cost wheat by-products	117:142	Low-cost wheat by-products	117:142	Low-cost wheat by-products have been modified to become an effective delivery system for curcumin.					
35917787	7	34	theme	%	1154:1154	arg1	release					1120:1126	a final release	1112:1126	a final release of curcumin of 77.8 ± 2.3 %	1112:1154	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	6	35	theme	structural	913:922	arg1	co-assembly					924:934	their structural co-assembly	907:934	their structural co-assembly	907:934	To improve the bioavailability of curcumin, it was encapsulated in WWBCs composites by participating in their structural co-assembly.					
35917787	5	36	theme	TEM	666:668	arg1	characterization					670:685	TEM characterization	666:685	TEM characterization	666:685	The nanocomplexes had a typical core-shell structure according to TEM characterization, where proteins aggregate to form a hydrophobic core and the hydrophilic WBC and STP crosslinked to form the shell.					
35917787	2	37	theme	Wheat	216:220	arg1	bran					222:225	Wheat bran	216:225	Wheat bran cellulose (WBC)	216:241	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	2	38	theme	bran	222:225	arg1	WBC					238:240	WBC	238:240	WBC	238:240	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	2	38	theme	bran	222:225	arg1	cellulose					227:235	Wheat bran cellulose	216:235	Wheat bran cellulose (WBC)	216:241	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	1	39	theme	wheat	126:130	arg1	by-products					132:142	Low-cost wheat by-products	117:142	Low-cost wheat by-products	117:142	Low-cost wheat by-products have been modified to become an effective delivery system for curcumin.					
35917787	7	40	dep	In	937:938	arg1	vitro					940:944	vitro	940:944	vitro	940:944	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	2	41	theme	gluten	253:258	arg1	WPs					270:272	WPs	270:272	WPs	270:272	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	2	41	theme	gluten	253:258	arg1	proteins					260:267	Wheat bran cellulose (WBC) and wheat gluten proteins	216:267	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs)	216:273	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	4	42	theme	co-assembled	576:587	arg1	structure					589:597	the co-assembled structure	572:597	the co-assembled structure	572:597	Modifying the nanocomposite with STP lowered the Dh and PDI of the co-assembled structure.					
35917787	2	43	theme	wheat	247:251	arg1	WPs					270:272	WPs	270:272	WPs	270:272	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	2	43	theme	wheat	247:251	arg1	proteins					260:267	Wheat bran cellulose (WBC) and wheat gluten proteins	216:267	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs)	216:273	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	7	44	theme	curcumin	1131:1138	arg1	release					1120:1126	a final release	1112:1126	a final release of curcumin of 77.8 ± 2.3 %	1112:1154	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	2	45	theme	pH	298:299	arg1	cycle					301:305	a pH cycle	296:305	a pH cycle	296:305	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	0	46	dep	co-assembly	12:22	arg1	cellulose					64:72	cellulose	64:72	cellulose	64:72	Hydrophilic co-assembly of wheat gluten proteins and wheat bran cellulose improving the bioavailability of curcumin.					
35917787	3	47	theme	unfolding	492:500	arg1	state					502:506	a relative unfolding state	481:506	a relative unfolding state	481:506	Fluorescence spectroscopy and zeta-potential evidenced that the embedding of WBC into the WPs favored the formation composites a relative unfolding state.					
35917787	7	48	theme	digestion	973:981	arg1	experiments					983:993	In vitro simulated gastrointestinal digestion experiments	937:993	In vitro simulated gastrointestinal digestion experiments	937:993	In vitro simulated gastrointestinal digestion experiments showed that the curcumin encapsulated in WWBCs possessed gastrointestinal slow and controlled release function, with a final release of curcumin of 77.8 ± 2.3 %.					
35917787	2	49	theme	tripolyphosphate	330:345	arg1	addition					311:318	addition	311:318	addition of sodium tripolyphosphate (STP)	311:351	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	2	49	theme	tripolyphosphate	330:345	arg1	cycle					301:305	a pH cycle	296:305	a pH cycle	296:305	Wheat bran cellulose (WBC) and wheat gluten proteins (WPs) were co-assembled by a pH cycle and addition of sodium tripolyphosphate (STP).					
35917787	4	50	theme	structure	589:597	arg1	PDI					565:567	PDI	565:567	PDI	565:567	Modifying the nanocomposite with STP lowered the Dh and PDI of the co-assembled structure.					
35917787	4	50	theme	structure	589:597	arg1	Dh					558:559	Dh	558:559	Dh	558:559	Modifying the nanocomposite with STP lowered the Dh and PDI of the co-assembled structure.					
35843041	7	0	theme	mass	913:916	arg1	evaluation					929:938	A lean mass (LM) index evaluation	906:938	A lean mass (LM) index evaluation	906:938	A lean mass (LM) index evaluation showed no LM deficits in relation to corresponding reference populations.					
35843041	6	1	theme	FM	762:763	arg1	index					766:770	An elevated fat mass (FM) index	740:770	An elevated fat mass (FM) index	740:770	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	2	theme	y	834:834	arg1	Ia					853:854	age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia	804:854	Ia	853:854	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	2	theme	y	834:834	arg1	patients					794:801	16 of 21 patients	785:801	16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1)	785:903	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	1	3	theme	glycogen	148:155	arg1	GSDs					176:179	GSDs	176:179	GSDs	176:179	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	1	3	theme	glycogen	148:155	arg1	disorders					165:173	hepatic glycogen storage disorders	140:173	hepatic glycogen storage disorders (GSDs)	140:180	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	12	4	with	treatment	1431:1439	arg1	UCCS					1446:1449	UCCS	1446:1449	UCCS	1446:1449	We also suggest that treatment with UCCS is associated with excess weight in these patients.					
35843041	11	5	from	frequency	1354:1362	arg1	patients					1382:1389	patients	1382:1389	patients with hepatic GSDs	1382:1407	CONCLUSIONS These findings suggest a high frequency of elevated FM in patients with hepatic GSDs.					
35843041	0	6	from	composition	5:15	arg1	patients					20:27	patients	20:27	patients with hepatic glycogen storage diseases	20:66	Body composition in patients with hepatic glycogen storage diseases.					
35843041	1	7	theme	storage	157:163	arg1	GSDs					176:179	GSDs	176:179	GSDs	176:179	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	1	7	theme	storage	157:163	arg1	disorders					165:173	hepatic glycogen storage disorders	140:173	hepatic glycogen storage disorders (GSDs)	140:180	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	11	8	theme	elevated	1367:1374	arg1	FM					1376:1377	elevated FM	1367:1377	elevated FM	1367:1377	CONCLUSIONS These findings suggest a high frequency of elevated FM in patients with hepatic GSDs.					
35843041	5	9	theme	composition	581:591	arg1	findings					593:600	Body composition findings	576:600	Body composition findings	576:600	Body composition findings were correlated with clinical parameters, uncooked cornstarch (UCCS) regimen, dietary intake, and markers of treatment adherence.					
35843041	6	10	theme	mass	756:759	arg1	index					766:770	An elevated fat mass (FM) index	740:770	An elevated fat mass (FM) index	740:770	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	8	11	dep	patients	1083:1090	arg1	Ib					1098:1099	type Ib	1093:1099	type Ib: n = 1 and IX-α: n = 1	1093:1122	Relative skeletal muscle index values were decreased in 2 of 7 adult patients (type Ib: n = 1 and IX-α: n = 1).					
35843041	6	12	theme	age	804:806	arg1	y					813:813	age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia	804:854	y	813:813	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	12	theme	age	804:806	arg1	Ib					865:866	Ib	865:866	Ib	865:866	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	12	theme	age	804:806	arg1	patients					794:801	16 of 21 patients	785:801	16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1)	785:903	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	7	13	theme	LM	950:951	arg1	deficits					953:960	no LM deficits	947:960	no LM deficits	947:960	A lean mass (LM) index evaluation showed no LM deficits in relation to corresponding reference populations.					
35843041	6	14	theme	fat	752:754	arg1	index					766:770	An elevated fat mass (FM) index	740:770	An elevated fat mass (FM) index	740:770	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	8	15	theme	index	1039:1043	arg1	values					1045:1050	Relative skeletal muscle index values	1014:1050	Relative skeletal muscle index values	1014:1050	Relative skeletal muscle index values were decreased in 2 of 7 adult patients (type Ib: n = 1 and IX-α: n = 1).					
35843041	13	16	theme	protein	1551:1557	arg1	intake					1559:1564	protein intake	1551:1564	protein intake	1551:1564	Additionally, the treatment strategy can impair protein intake, and lead to a decrease in LM.					
35843041	4	17	theme	energy	547:552	arg1	absorptiometry					560:573	dual energy x-ray absorptiometry	542:573	dual energy x-ray absorptiometry	542:573	Three-day dietary records were collected in the week preceding dual energy x-ray absorptiometry.					
35843041	8	18	dep	Ib	1098:1099	arg1	n = 1					1118:1122	n = 1	1118:1122	type Ib: n = 1 and IX-α: n = 1	1093:1122	Relative skeletal muscle index values were decreased in 2 of 7 adult patients (type Ib: n = 1 and IX-α: n = 1).					
35843041	8	18	dep	Ib	1098:1099	arg1	IX-α					1112:1115	IX-α	1112:1115	IX-α	1112:1115	Relative skeletal muscle index values were decreased in 2 of 7 adult patients (type Ib: n = 1 and IX-α: n = 1).					
35843041	8	18	dep	Ib	1098:1099	arg1	n = 1					1102:1106	n = 1	1102:1106	n = 1	1102:1106	Relative skeletal muscle index values were decreased in 2 of 7 adult patients (type Ib: n = 1 and IX-α: n = 1).					
35843041	6	19	theme	elevated	743:750	arg1	index					766:770	An elevated fat mass (FM) index	740:770	An elevated fat mass (FM) index	740:770	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	12	20	theme	excess	1470:1475	arg1	weight					1477:1482	excess weight	1470:1482	excess weight in these patients	1470:1500	We also suggest that treatment with UCCS is associated with excess weight in these patients.					
35843041	6	21	theme	type	848:851	arg1	Ia					853:854	age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia	804:854	Ia	853:854	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	21	theme	type	848:851	arg1	patients					794:801	16 of 21 patients	785:801	16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1)	785:903	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	11	22	dep	CONCLUSIONS	1312:1322	arg1	suggest					1339:1345	suggest	1339:1345	suggest a high frequency of elevated FM in patients with hepatic GSDs	1339:1407	CONCLUSIONS These findings suggest a high frequency of elevated FM in patients with hepatic GSDs.					
35843041	5	23	theme	adherence	721:729	arg1	regimen					671:677	uncooked cornstarch (UCCS) regimen	644:677	uncooked cornstarch (UCCS) regimen	644:677	Body composition findings were correlated with clinical parameters, uncooked cornstarch (UCCS) regimen, dietary intake, and markers of treatment adherence.					
35843041	5	23	theme	adherence	721:729	arg1	intake					688:693	dietary intake	680:693	dietary intake	680:693	Body composition findings were correlated with clinical parameters, uncooked cornstarch (UCCS) regimen, dietary intake, and markers of treatment adherence.					
35843041	5	23	theme	adherence	721:729	arg1	markers					700:706	markers	700:706	markers of treatment adherence	700:729	Body composition findings were correlated with clinical parameters, uncooked cornstarch (UCCS) regimen, dietary intake, and markers of treatment adherence.					
35843041	5	23	theme	adherence	721:729	arg1	parameters					632:641	clinical parameters	623:641	clinical parameters	623:641	Body composition findings were correlated with clinical parameters, uncooked cornstarch (UCCS) regimen, dietary intake, and markers of treatment adherence.					
35843041	1	24	theme	disorders	165:173	arg1	composition					125:135	the body composition	116:135	the body composition of hepatic glycogen storage disorders (GSDs)	116:180	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	5	25	theme	UCCS	665:668	arg1	regimen					671:677	uncooked cornstarch (UCCS) regimen	644:677	uncooked cornstarch (UCCS) regimen	644:677	Body composition findings were correlated with clinical parameters, uncooked cornstarch (UCCS) regimen, dietary intake, and markers of treatment adherence.					
35843041	5	26	theme	uncooked	644:651	arg1	regimen					671:677	uncooked cornstarch (UCCS) regimen	644:677	uncooked cornstarch (UCCS) regimen	644:677	Body composition findings were correlated with clinical parameters, uncooked cornstarch (UCCS) regimen, dietary intake, and markers of treatment adherence.					
35843041	10	27	theme	relative	1212:1219	arg1	weight					1244:1249	g/kg body weight	1234:1249	g/kg body weight	1234:1249	In contrast, relative UCCS intake (g/kg body weight) was negatively associated with LM/kg (rs = -0.8; P ≤ 0.01).					
35843041	10	27	theme	relative	1212:1219	arg1	intake					1226:1231	relative UCCS intake	1212:1231	relative UCCS intake (g/kg body weight)	1212:1250	In contrast, relative UCCS intake (g/kg body weight) was negatively associated with LM/kg (rs = -0.8; P ≤ 0.01).					
35843041	5	28	theme	treatment	711:719	arg1	adherence					721:729	treatment adherence	711:729	treatment adherence	711:729	Body composition findings were correlated with clinical parameters, uncooked cornstarch (UCCS) regimen, dietary intake, and markers of treatment adherence.					
35843041	10	29	theme	UCCS	1221:1224	arg1	weight					1244:1249	g/kg body weight	1234:1249	g/kg body weight	1234:1249	In contrast, relative UCCS intake (g/kg body weight) was negatively associated with LM/kg (rs = -0.8; P ≤ 0.01).					
35843041	10	29	theme	UCCS	1221:1224	arg1	intake					1226:1231	relative UCCS intake	1212:1231	relative UCCS intake (g/kg body weight)	1212:1250	In contrast, relative UCCS intake (g/kg body weight) was negatively associated with LM/kg (rs = -0.8; P ≤ 0.01).					
35843041	13	30	from	decrease	1581:1588	arg1	LM					1593:1594	LM	1593:1594	LM	1593:1594	Additionally, the treatment strategy can impair protein intake, and lead to a decrease in LM.					
35843041	1	31	theme	present	84:90	arg1	study					92:96	The present study	80:96	The present study	80:96	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	3	32	with	patients	307:314	arg1	GSD					321:323	GSD	321:323	GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7)	321:462	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	0	33	theme	Body	0:3	arg1	composition					5:15	Body composition	0:15	Body composition in patients with hepatic glycogen storage diseases.	0:67	Body composition in patients with hepatic glycogen storage diseases.					
35843041	11	34	theme	high	1349:1352	arg1	frequency					1354:1362	a high frequency	1347:1362	a high frequency of elevated FM in patients with hepatic GSDs	1347:1407	CONCLUSIONS These findings suggest a high frequency of elevated FM in patients with hepatic GSDs.					
35843041	11	35	with	patients	1382:1389	arg1	GSDs					1404:1407	hepatic GSDs	1396:1407	hepatic GSDs	1396:1407	CONCLUSIONS These findings suggest a high frequency of elevated FM in patients with hepatic GSDs.					
35843041	2	36	dep	exploratory	244:254	arg1	cross-sectional					272:286	cross-sectional	272:286	cross-sectional	272:286	METHODS This was an exploratory, observational, cross-sectional study.					
35843041	2	36	dep	exploratory	244:254	arg1	observational					257:269	observational	257:269	observational	257:269	METHODS This was an exploratory, observational, cross-sectional study.					
35843041	13	37	theme	treatment	1521:1529	arg1	strategy					1531:1538	the treatment strategy	1517:1538	the treatment strategy	1517:1538	Additionally, the treatment strategy can impair protein intake, and lead to a decrease in LM.					
35843041	3	38	dep	GSD	321:323	arg1	Ia					331:332	type Ia	326:332	type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7	326:461	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	12	39	from	weight	1477:1482	arg1	patients					1493:1500	these patients	1487:1500	these patients	1487:1500	We also suggest that treatment with UCCS is associated with excess weight in these patients.					
35843041	8	40	theme	skeletal	1023:1030	arg1	values					1045:1050	Relative skeletal muscle index values	1014:1050	Relative skeletal muscle index values	1014:1050	Relative skeletal muscle index values were decreased in 2 of 7 adult patients (type Ib: n = 1 and IX-α: n = 1).					
35843041	6	41	located	found	776:780	arg2	index					766:770	An elevated fat mass (FM) index	740:770	An elevated fat mass (FM) index	740:770	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	41	located	found	776:780	arg1	Ia					853:854	age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia	804:854	Ia	853:854	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	41	located	found	776:780	arg1	y					813:813	age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia	804:854	y	813:813	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	41	located	found	776:780	arg1	patients					794:801	16 of 21 patients	785:801	16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1)	785:903	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	4	42	theme	Three-day	479:487	arg1	records					497:503	Three-day dietary records	479:503	Three-day dietary records	479:503	Three-day dietary records were collected in the week preceding dual energy x-ray absorptiometry.					
35843041	5	43	theme	dietary	680:686	arg1	intake					688:693	dietary intake	680:693	dietary intake	680:693	Body composition findings were correlated with clinical parameters, uncooked cornstarch (UCCS) regimen, dietary intake, and markers of treatment adherence.					
35843041	11	44	theme	FM	1376:1377	arg1	frequency					1354:1362	a high frequency	1347:1362	a high frequency of elevated FM in patients with hepatic GSDs	1347:1407	CONCLUSIONS These findings suggest a high frequency of elevated FM in patients with hepatic GSDs.					
35843041	9	45	dep	≤	1190:1190	arg1	rs = 0.7					1178:1185	rs = 0.7	1178:1185	rs = 0.7	1178:1185	UCCS (g/d) correlated positively with the FM index (rs = 0.7; P ≤ 0.01).					
35843041	6	46	dep	y	813:813	arg1	n = 12					857:862	n = 12	857:862	n = 12	857:862	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	46	dep	y	813:813	arg1	n = 1					881:885	n = 1	881:885	n = 1	881:885	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	46	dep	y	813:813	arg1	IX-γ					892:895	IX-γ	892:895	IX-γ	892:895	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	46	dep	y	813:813	arg1	n = 2					869:873	n = 2	869:873	n = 2	869:873	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	46	dep	y	813:813	arg1	n = 1					898:902	n = 1	898:902	n = 1	898:902	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	4	47	theme	dual	542:545	arg1	absorptiometry					560:573	dual energy x-ray absorptiometry	542:573	dual energy x-ray absorptiometry	542:573	Three-day dietary records were collected in the week preceding dual energy x-ray absorptiometry.					
35843041	1	48	theme	dual	190:193	arg1	absorptiometry					208:221	dual energy x-ray absorptiometry	190:221	dual energy x-ray absorptiometry	190:221	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	0	49	theme	hepatic	34:40	arg1	diseases					59:66	hepatic glycogen storage diseases	34:66	hepatic glycogen storage diseases	34:66	Body composition in patients with hepatic glycogen storage diseases.					
35843041	7	50	theme	corresponding	977:989	arg1	populations					1001:1011	corresponding reference populations	977:1011	corresponding reference populations	977:1011	A lean mass (LM) index evaluation showed no LM deficits in relation to corresponding reference populations.					
35843041	1	51	theme	energy	195:200	arg1	absorptiometry					208:221	dual energy x-ray absorptiometry	190:221	dual energy x-ray absorptiometry	190:221	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	4	52	theme	dietary	489:495	arg1	records					497:503	Three-day dietary records	479:503	Three-day dietary records	479:503	Three-day dietary records were collected in the week preceding dual energy x-ray absorptiometry.					
35843041	3	53	theme	female	387:392	arg1	sex					394:396	female sex	387:396	female sex	387:396	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	5	54	theme	cornstarch	653:662	arg1	regimen					671:677	uncooked cornstarch (UCCS) regimen	644:677	uncooked cornstarch (UCCS) regimen	644:677	Body composition findings were correlated with clinical parameters, uncooked cornstarch (UCCS) regimen, dietary intake, and markers of treatment adherence.					
35843041	5	55	theme	Body	576:579	arg1	composition					581:591	Body composition	576:591	Body composition findings	576:600	Body composition findings were correlated with clinical parameters, uncooked cornstarch (UCCS) regimen, dietary intake, and markers of treatment adherence.					
35843041	3	56	dep	Ia	331:332	arg1	y					454:454	19 y	451:454	type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7	326:461	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	3	56	dep	Ia	331:332	arg1	y					417:417	8 y	415:417	8 y	415:417	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	3	56	dep	Ia	331:332	arg1	n = 14					435:440	n = 14	435:440	n = 14	435:440	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	3	56	dep	Ia	331:332	arg1	n = 4					380:384	n = 4	380:384	n = 4	380:384	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	3	56	dep	Ia	331:332	arg1	n = 13					335:340	n = 13	335:340	n = 13	335:340	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	3	56	dep	Ia	331:332	arg1	n = 7					457:461	n = 7	457:461	type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7	326:461	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	3	56	dep	Ia	331:332	arg1	Ib					343:344	Ib	343:344	Ib	343:344	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	3	56	dep	Ia	331:332	arg1	&lt					411:413	age &lt	407:413	age &lt	407:413	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	3	56	dep	Ia	331:332	arg1	y					432:432	n = 3, 8-19 y	420:432	y	432:432	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	3	56	dep	Ia	331:332	arg1	sex					394:396	female sex	387:396	female sex	387:396	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	3	56	dep	Ia	331:332	arg1	&gt					447:449	&gt	447:449	&gt	447:449	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	3	56	dep	Ia	331:332	arg1	n = 5					347:351	n = 5	347:351	n = 5	347:351	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	3	56	dep	Ia	331:332	arg1	n = 13					399:404	n = 13	399:404	n = 13	399:404	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	8	57	theme	adult	1077:1081	arg1	patients					1083:1090	2 of 7 adult patients	1070:1090	2 of 7 adult patients (type Ib: n = 1 and IX-α: n = 1)	1070:1123	Relative skeletal muscle index values were decreased in 2 of 7 adult patients (type Ib: n = 1 and IX-α: n = 1).					
35843041	0	58	with	patients	20:27	arg1	diseases					59:66	hepatic glycogen storage diseases	34:66	hepatic glycogen storage diseases	34:66	Body composition in patients with hepatic glycogen storage diseases.					
35843041	2	59	dep	METHODS	224:230	arg1	This					232:235	This	232:235	This	232:235	METHODS This was an exploratory, observational, cross-sectional study.					
35843041	0	60	theme	storage	51:57	arg1	diseases					59:66	hepatic glycogen storage diseases	34:66	hepatic glycogen storage diseases	34:66	Body composition in patients with hepatic glycogen storage diseases.					
35843041	8	61	theme	type	1093:1096	arg1	Ib					1098:1099	type Ib	1093:1099	type Ib: n = 1 and IX-α: n = 1	1093:1122	Relative skeletal muscle index values were decreased in 2 of 7 adult patients (type Ib: n = 1 and IX-α: n = 1).					
35843041	1	62	dep	OBJECTIVES	69:78	arg1	aimed					98:102	aimed	98:102	aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry	98:221	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	0	63	theme	glycogen	42:49	arg1	diseases					59:66	hepatic glycogen storage diseases	34:66	hepatic glycogen storage diseases	34:66	Body composition in patients with hepatic glycogen storage diseases.					
35843041	11	64	theme	hepatic	1396:1402	arg1	GSDs					1404:1407	hepatic GSDs	1396:1407	hepatic GSDs	1396:1407	CONCLUSIONS These findings suggest a high frequency of elevated FM in patients with hepatic GSDs.					
35843041	4	65	theme	x-ray	554:558	arg1	absorptiometry					560:573	dual energy x-ray absorptiometry	542:573	dual energy x-ray absorptiometry	542:573	Three-day dietary records were collected in the week preceding dual energy x-ray absorptiometry.					
35843041	10	66	theme	body	1239:1242	arg1	weight					1244:1249	g/kg body weight	1234:1249	g/kg body weight	1234:1249	In contrast, relative UCCS intake (g/kg body weight) was negatively associated with LM/kg (rs = -0.8; P ≤ 0.01).					
35843041	10	66	theme	body	1239:1242	arg1	intake					1226:1231	relative UCCS intake	1212:1231	relative UCCS intake (g/kg body weight)	1212:1250	In contrast, relative UCCS intake (g/kg body weight) was negatively associated with LM/kg (rs = -0.8; P ≤ 0.01).					
35843041	9	67	theme	FM	1168:1169	arg1	index					1171:1175	the FM index	1164:1175	the FM index	1164:1175	UCCS (g/d) correlated positively with the FM index (rs = 0.7; P ≤ 0.01).					
35843041	1	68	theme	body	120:123	arg1	composition					125:135	the body composition	116:135	the body composition of hepatic glycogen storage disorders (GSDs)	116:180	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	10	69	dep	≤	1303:1303	arg1	rs = -0.8					1290:1298	rs = -0.8	1290:1298	rs = -0.8	1290:1298	In contrast, relative UCCS intake (g/kg body weight) was negatively associated with LM/kg (rs = -0.8; P ≤ 0.01).					
35843041	6	70	dep	patients	794:801	arg1	Ia					853:854	age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia	804:854	Ia	853:854	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	70	dep	patients	794:801	arg1	y					813:813	age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia	804:854	y	813:813	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	70	dep	patients	794:801	arg1	Ib					865:866	Ib	865:866	Ib	865:866	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	6	70	dep	patients	794:801	arg1	patients					794:801	16 of 21 patients	785:801	16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1)	785:903	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	2	71	theme	exploratory	244:254	arg1	METHODS					224:230	METHODS	224:230	METHODS This	224:235	METHODS This was an exploratory, observational, cross-sectional study.					
35843041	2	71	theme	exploratory	244:254	arg1	study					288:292	an exploratory, observational, cross-sectional study	241:292	an exploratory, observational, cross-sectional study	241:292	METHODS This was an exploratory, observational, cross-sectional study.					
35843041	10	72	theme	g/kg	1234:1237	arg1	weight					1244:1249	g/kg body weight	1234:1249	g/kg body weight	1234:1249	In contrast, relative UCCS intake (g/kg body weight) was negatively associated with LM/kg (rs = -0.8; P ≤ 0.01).					
35843041	10	72	theme	g/kg	1234:1237	arg1	intake					1226:1231	relative UCCS intake	1212:1231	relative UCCS intake (g/kg body weight)	1212:1250	In contrast, relative UCCS intake (g/kg body weight) was negatively associated with LM/kg (rs = -0.8; P ≤ 0.01).					
35843041	3	73	theme	age	407:409	arg1	&lt					411:413	age &lt	407:413	age &lt	407:413	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	8	74	theme	muscle	1032:1037	arg1	values					1045:1050	Relative skeletal muscle index values	1014:1050	Relative skeletal muscle index values	1014:1050	Relative skeletal muscle index values were decreased in 2 of 7 adult patients (type Ib: n = 1 and IX-α: n = 1).					
35843041	6	75	dep	RESULTS	732:738	arg1	found					776:780	found	776:780	was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1)	772:903	RESULTS An elevated fat mass (FM) index was found in 16 of 21 patients (age 8-19 y: n = 10 and &gt;19 y: n = 6; GSD type Ia: n = 12, Ib: n = 2, III: n = 1, and IX-γ: n = 1).					
35843041	3	76	theme	type	326:329	arg1	Ia					331:332	type Ia	326:332	type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7	326:461	Twenty-four patients with GSD (type Ia: n = 13, Ib: n = 5, III: n = 2, and IX-α/β/γ: n = 4; female sex: n = 13; age &lt;8 y: n = 3, 8-19 y: n = 14, and &gt;19 y: n = 7) were included.					
35843041	1	77	theme	x-ray	202:206	arg1	absorptiometry					208:221	dual energy x-ray absorptiometry	190:221	dual energy x-ray absorptiometry	190:221	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	7	78	theme	reference	991:999	arg1	populations					1001:1011	corresponding reference populations	977:1011	corresponding reference populations	977:1011	A lean mass (LM) index evaluation showed no LM deficits in relation to corresponding reference populations.					
35843041	8	79	theme	Relative	1014:1021	arg1	values					1045:1050	Relative skeletal muscle index values	1014:1050	Relative skeletal muscle index values	1014:1050	Relative skeletal muscle index values were decreased in 2 of 7 adult patients (type Ib: n = 1 and IX-α: n = 1).					
35843041	5	80	theme	clinical	623:630	arg1	parameters					632:641	clinical parameters	623:641	clinical parameters	623:641	Body composition findings were correlated with clinical parameters, uncooked cornstarch (UCCS) regimen, dietary intake, and markers of treatment adherence.					
35843041	1	81	theme	hepatic	140:146	arg1	GSDs					176:179	GSDs	176:179	GSDs	176:179	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	1	81	theme	hepatic	140:146	arg1	disorders					165:173	hepatic glycogen storage disorders	140:173	hepatic glycogen storage disorders (GSDs)	140:180	OBJECTIVES The present study aimed to evaluate the body composition of hepatic glycogen storage disorders (GSDs) through dual energy x-ray absorptiometry.					
35843041	7	82	theme	index	923:927	arg1	evaluation					929:938	A lean mass (LM) index evaluation	906:938	A lean mass (LM) index evaluation	906:938	A lean mass (LM) index evaluation showed no LM deficits in relation to corresponding reference populations.					
35843041	7	83	theme	lean	908:911	arg1	LM					919:920	LM	919:920	LM	919:920	A lean mass (LM) index evaluation showed no LM deficits in relation to corresponding reference populations.					
35843041	7	83	theme	lean	908:911	arg1	mass					913:916	A lean mass	906:916	A lean mass (LM) index evaluation	906:938	A lean mass (LM) index evaluation showed no LM deficits in relation to corresponding reference populations.					
36587550	0	0	theme	feed	87:90	arg1	quality					38:44	the quality	34:44	the quality of chrysanthemum waste	34:67	Solid-state fermentation improves the quality of chrysanthemum waste as an alternative feed ingredient.					
36587550	0	0	theme	feed	87:90	arg1	ingredient					92:101	an alternative feed ingredient	72:101	an alternative feed ingredient	72:101	Solid-state fermentation improves the quality of chrysanthemum waste as an alternative feed ingredient.					
36587550	5	1	theme	acid	1123:1126	arg1	profiles					1128:1135	the free and hydrolyzed amino acid profiles	1093:1135	the free and hydrolyzed amino acid profiles of FCW	1093:1142	SSF improved the free and hydrolyzed amino acid profiles of FCW, increased the content of flavor amino acids, and improved the amino acid composition of FCW protein.					
36587550	2	2	theme	serious	346:352	arg1	problems					368:375	serious environmental problems	346:375	serious environmental problems	346:375	Although it is nutritious, most CW is discarded, wasting resources and contributing to serious environmental problems.					
36587550	5	3	theme	acids	1183:1187	arg1	content					1159:1165	the content	1155:1165	the content of flavor amino acids	1155:1187	SSF improved the free and hydrolyzed amino acid profiles of FCW, increased the content of flavor amino acids, and improved the amino acid composition of FCW protein.					
36587550	0	4	theme	alternative	75:85	arg1	quality					38:44	the quality	34:44	the quality of chrysanthemum waste	34:67	Solid-state fermentation improves the quality of chrysanthemum waste as an alternative feed ingredient.					
36587550	0	4	theme	alternative	75:85	arg1	ingredient					92:101	an alternative feed ingredient	72:101	an alternative feed ingredient	72:101	Solid-state fermentation improves the quality of chrysanthemum waste as an alternative feed ingredient.					
36587550	4	5	theme	Orthogonal	504:513	arg1	experiment					515:524	Orthogonal experiment	504:524	Orthogonal experiment	504:524	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	1	6	theme	industrial	152:161	arg1	by-product					163:172	an agricultural and industrial by-product	132:172	an agricultural and industrial by-product produced during chrysanthemum harvesting, drying, preservation, and deep processing	132:256	Chrysanthemum waste (CW) is an agricultural and industrial by-product produced during chrysanthemum harvesting, drying, preservation, and deep processing.					
36587550	1	6	theme	industrial	152:161	arg1	waste					118:122	Chrysanthemum waste	104:122	Chrysanthemum waste (CW)	104:127	Chrysanthemum waste (CW) is an agricultural and industrial by-product produced during chrysanthemum harvesting, drying, preservation, and deep processing.					
36587550	4	7	theme	chrysanthemum	575:587	arg1	FCW					596:598	FCW	596:598	FCW	596:598	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	7	theme	chrysanthemum	575:587	arg1	waste					589:593	fermented chrysanthemum waste	565:593	fermented chrysanthemum waste (FCW)	565:599	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	7	8	theme	chrysanthemum	1372:1384	arg1	processing					1386:1395	chrysanthemum processing	1372:1395	chrysanthemum processing	1372:1395	FCW shows potential use as a feed ingredient, and SSF helps reduce the waste of chrysanthemum processing.					
36587550	4	9	theme	protein	874:880	arg1	contents					856:863	the contents	852:863	the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01),	852:1015	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	2	10	theme	most	286:289	arg1	CW					291:292	most CW	286:292	most CW	286:292	Although it is nutritious, most CW is discarded, wasting resources and contributing to serious environmental problems.					
36587550	6	11	theme	nutritional	1271:1281	arg1	quality					1283:1289	CW nutritional quality	1268:1289	CW nutritional quality	1268:1289	Overall, SSF improved CW nutritional quality.					
36587550	4	12	theme	detergent	916:924	arg1	fiber					926:930	acid detergent fiber	911:930	acid detergent fiber	911:930	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	12	theme	detergent	916:924	arg1	protein					874:880	crude protein	868:880	crude protein	868:880	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	13	theme	crude	868:872	arg1	fiber					904:908	crude fiber	898:908	crude fiber	898:908	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	13	theme	crude	868:872	arg1	phosphorus					972:981	phosphorus	972:981	phosphorus	972:981	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	13	theme	crude	868:872	arg1	fiber					951:955	neutral detergent fiber	933:955	neutral detergent fiber	933:955	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	13	theme	crude	868:872	arg1	ash					958:960	ash	958:960	ash	958:960	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	13	theme	crude	868:872	arg1	extract					889:895	ether extract	883:895	ether extract	883:895	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	13	theme	crude	868:872	arg1	fiber					926:930	acid detergent fiber	911:930	acid detergent fiber	911:930	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	13	theme	crude	868:872	arg1	flavonoids					994:1003	total flavonoids	988:1003	total flavonoids	988:1003	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	13	theme	crude	868:872	arg1	calcium					963:969	calcium	963:969	calcium	963:969	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	13	theme	crude	868:872	arg1	protein					874:880	crude protein	868:880	crude protein	868:880	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	3	14	theme	feed	487:490	arg1	ingredient					492:501	an alternative feed ingredient	472:501	an alternative feed ingredient	472:501	This work explored a solid-state fermentation (SSF) strategy to improve CW quality for use as an alternative feed ingredient.					
36587550	4	15	theme	neutral	933:939	arg1	fiber					951:955	neutral detergent fiber	933:955	neutral detergent fiber	933:955	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	15	theme	neutral	933:939	arg1	p < 0.01					1006:1013	p < 0.01	1006:1013	p < 0.01	1006:1013	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	15	theme	neutral	933:939	arg1	protein					874:880	crude protein	868:880	crude protein	868:880	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	16	dep	tropicalis + Bacillus	672:692	arg1	amyloliquefaciens					694:710	amyloliquefaciens	694:710	amyloliquefaciens	694:710	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	17	theme	9:1	636:638	arg1	2:2:1					727:731	2:2:1	727:731	2:2:1	727:731	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	17	theme	9:1	636:638	arg1	ratio					627:631	cornmeal mass ratio	613:631	cornmeal mass ratio of 9:1	613:638	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	17	theme	9:1	636:638	arg1	time					777:780	fermentation time	764:780	fermentation time	764:780	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	17	theme	9:1	636:638	arg1	amount					746:751	inoculation amount	734:751	inoculation amount of 6%	734:757	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	17	theme	9:1	636:638	arg1	proportions					712:722	Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions	641:722	Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1	641:731	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	17	theme	9:1	636:638	arg1	9:1					636:638	9:1	636:638	9:1	636:638	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	17	theme	9:1	636:638	arg1	%					757:757	6%	756:757	6%	756:757	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	0	18	theme	chrysanthemum	49:61	arg1	waste					63:67	chrysanthemum waste	49:67	chrysanthemum waste	49:67	Solid-state fermentation improves the quality of chrysanthemum waste as an alternative feed ingredient.					
36587550	4	19	theme	fermentation	764:775	arg1	time					777:780	fermentation time	764:780	fermentation time	764:780	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	6	20	theme	CW	1268:1269	arg1	quality					1283:1289	CW nutritional quality	1268:1289	CW nutritional quality	1268:1289	Overall, SSF improved CW nutritional quality.					
36587550	4	21	theme	Pediococcus	641:651	arg1	proportions					712:722	Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions	641:722	Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1	641:731	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	0	22	theme	Solid-state	0:10	arg1	fermentation					12:23	Solid-state fermentation	0:23	Solid-state fermentation	0:23	Solid-state fermentation improves the quality of chrysanthemum waste as an alternative feed ingredient.					
36587550	5	23	theme	hydrolyzed	1106:1115	arg1	profiles					1128:1135	the free and hydrolyzed amino acid profiles	1093:1135	the free and hydrolyzed amino acid profiles of FCW	1093:1142	SSF improved the free and hydrolyzed amino acid profiles of FCW, increased the content of flavor amino acids, and improved the amino acid composition of FCW protein.					
36587550	7	24	theme	feed	1321:1324	arg1	ingredient					1326:1335	a feed ingredient	1319:1335	a feed ingredient	1319:1335	FCW shows potential use as a feed ingredient, and SSF helps reduce the waste of chrysanthemum processing.					
36587550	4	25	theme	saponin	1052:1058	arg1	content					1060:1066	saponin content	1052:1066	saponin content (p < 0.01)	1052:1077	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	25	theme	saponin	1052:1058	arg1	p < 0.01					1069:1076	p < 0.01	1069:1076	p < 0.01	1069:1076	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	1	26	theme	chrysanthemum	190:202	arg1	harvesting					204:213	chrysanthemum harvesting	190:213	chrysanthemum harvesting	190:213	Chrysanthemum waste (CW) is an agricultural and industrial by-product produced during chrysanthemum harvesting, drying, preservation, and deep processing.					
36587550	4	27	theme	optimal	542:548	arg1	conditions					550:559	the optimal conditions	538:559	the optimal conditions for fermented chrysanthemum waste (FCW)	538:599	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	1	28	theme	Chrysanthemum	104:116	arg1	by-product					163:172	an agricultural and industrial by-product	132:172	an agricultural and industrial by-product produced during chrysanthemum harvesting, drying, preservation, and deep processing	132:256	Chrysanthemum waste (CW) is an agricultural and industrial by-product produced during chrysanthemum harvesting, drying, preservation, and deep processing.					
36587550	1	28	theme	Chrysanthemum	104:116	arg1	CW					125:126	CW	125:126	CW	125:126	Chrysanthemum waste (CW) is an agricultural and industrial by-product produced during chrysanthemum harvesting, drying, preservation, and deep processing.					
36587550	1	28	theme	Chrysanthemum	104:116	arg1	waste					118:122	Chrysanthemum waste	104:122	Chrysanthemum waste (CW)	104:127	Chrysanthemum waste (CW) is an agricultural and industrial by-product produced during chrysanthemum harvesting, drying, preservation, and deep processing.					
36587550	4	29	theme	cornmeal	613:620	arg1	ratio					627:631	cornmeal mass ratio	613:631	cornmeal mass ratio of 9:1	613:638	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	30	theme	total	988:992	arg1	protein					874:880	crude protein	868:880	crude protein	868:880	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	30	theme	total	988:992	arg1	flavonoids					994:1003	total flavonoids	988:1003	total flavonoids	988:1003	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	31	theme	fermented	565:573	arg1	FCW					596:598	FCW	596:598	FCW	596:598	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	31	theme	fermented	565:573	arg1	waste					589:593	fermented chrysanthemum waste	565:593	fermented chrysanthemum waste (FCW)	565:599	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	32	theme	crude	898:902	arg1	fiber					904:908	crude fiber	898:908	crude fiber	898:908	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	32	theme	crude	898:902	arg1	protein					874:880	crude protein	868:880	crude protein	868:880	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	3	33	theme	fermentation	411:422	arg1	strategy					430:437	a solid-state fermentation (SSF) strategy	397:437	a solid-state fermentation (SSF) strategy	397:437	This work explored a solid-state fermentation (SSF) strategy to improve CW quality for use as an alternative feed ingredient.					
36587550	4	34	theme	acid	911:914	arg1	fiber					926:930	acid detergent fiber	911:930	acid detergent fiber	911:930	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	34	theme	acid	911:914	arg1	protein					874:880	crude protein	868:880	crude protein	868:880	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	35	theme	control	809:815	arg1	group					817:821	the control group	805:821	the control group	805:821	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	5	36	theme	protein	1237:1243	arg1	composition					1218:1228	the amino acid composition	1203:1228	the amino acid composition of FCW protein	1203:1243	SSF improved the free and hydrolyzed amino acid profiles of FCW, increased the content of flavor amino acids, and improved the amino acid composition of FCW protein.					
36587550	5	37	theme	FCW	1140:1142	arg1	profiles					1128:1135	the free and hydrolyzed amino acid profiles	1093:1135	the free and hydrolyzed amino acid profiles of FCW	1093:1142	SSF improved the free and hydrolyzed amino acid profiles of FCW, increased the content of flavor amino acids, and improved the amino acid composition of FCW protein.					
36587550	7	38	theme	potential	1302:1310	arg1	use					1312:1314	potential use	1302:1314	potential use as a feed ingredient	1302:1335	FCW shows potential use as a feed ingredient, and SSF helps reduce the waste of chrysanthemum processing.					
36587550	4	39	theme	mass	622:625	arg1	ratio					627:631	cornmeal mass ratio	613:631	cornmeal mass ratio of 9:1	613:638	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	0	40	theme	waste	63:67	arg1	ingredient					92:101	an alternative feed ingredient	72:101	an alternative feed ingredient	72:101	Solid-state fermentation improves the quality of chrysanthemum waste as an alternative feed ingredient.					
36587550	0	40	theme	waste	63:67	arg1	quality					38:44	the quality	34:44	the quality of chrysanthemum waste	34:67	Solid-state fermentation improves the quality of chrysanthemum waste as an alternative feed ingredient.					
36587550	4	41	theme	ether	883:887	arg1	extract					889:895	ether extract	883:895	ether extract	883:895	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	41	theme	ether	883:887	arg1	protein					874:880	crude protein	868:880	crude protein	868:880	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	5	42	theme	amino	1117:1121	arg1	profiles					1128:1135	the free and hydrolyzed amino acid profiles	1093:1135	the free and hydrolyzed amino acid profiles of FCW	1093:1142	SSF improved the free and hydrolyzed amino acid profiles of FCW, increased the content of flavor amino acids, and improved the amino acid composition of FCW protein.					
36587550	4	43	theme	2:2:1	727:731	arg1	2:2:1					727:731	2:2:1	727:731	2:2:1	727:731	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	43	theme	2:2:1	727:731	arg1	ratio					627:631	cornmeal mass ratio	613:631	cornmeal mass ratio of 9:1	613:638	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	43	theme	2:2:1	727:731	arg1	time					777:780	fermentation time	764:780	fermentation time	764:780	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	43	theme	2:2:1	727:731	arg1	amount					746:751	inoculation amount	734:751	inoculation amount of 6%	734:757	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	43	theme	2:2:1	727:731	arg1	proportions					712:722	Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions	641:722	Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1	641:731	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	43	theme	2:2:1	727:731	arg1	9:1					636:638	9:1	636:638	9:1	636:638	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	43	theme	2:2:1	727:731	arg1	%					757:757	6%	756:757	6%	756:757	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	3	44	theme	solid-state	399:409	arg1	SSF					425:427	SSF	425:427	SSF	425:427	This work explored a solid-state fermentation (SSF) strategy to improve CW quality for use as an alternative feed ingredient.					
36587550	3	44	theme	solid-state	399:409	arg1	fermentation					411:422	solid-state fermentation	399:422	a solid-state fermentation (SSF) strategy	397:437	This work explored a solid-state fermentation (SSF) strategy to improve CW quality for use as an alternative feed ingredient.					
36587550	5	45	theme	free	1097:1100	arg1	profiles					1128:1135	the free and hydrolyzed amino acid profiles	1093:1135	the free and hydrolyzed amino acid profiles of FCW	1093:1142	SSF improved the free and hydrolyzed amino acid profiles of FCW, increased the content of flavor amino acids, and improved the amino acid composition of FCW protein.					
36587550	3	46	theme	alternative	475:485	arg1	ingredient					492:501	an alternative feed ingredient	472:501	an alternative feed ingredient	472:501	This work explored a solid-state fermentation (SSF) strategy to improve CW quality for use as an alternative feed ingredient.					
36587550	4	47	theme	10 d.	785:789	arg1	CW					607:608	CW	607:608	CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d.	607:789	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	48	theme	cellaris + Candida	653:670	arg1	proportions					712:722	Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions	641:722	Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1	641:731	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	49	theme	inoculation	734:744	arg1	9:1					636:638	9:1	636:638	9:1	636:638	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	49	theme	inoculation	734:744	arg1	amount					746:751	inoculation amount	734:751	inoculation amount of 6%	734:757	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	49	theme	inoculation	734:744	arg1	%					757:757	6%	756:757	6%	756:757	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	49	theme	inoculation	734:744	arg1	2:2:1					727:731	2:2:1	727:731	2:2:1	727:731	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	50	theme	%	757:757	arg1	2:2:1					727:731	2:2:1	727:731	2:2:1	727:731	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	50	theme	%	757:757	arg1	ratio					627:631	cornmeal mass ratio	613:631	cornmeal mass ratio of 9:1	613:638	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	50	theme	%	757:757	arg1	time					777:780	fermentation time	764:780	fermentation time	764:780	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	50	theme	%	757:757	arg1	amount					746:751	inoculation amount	734:751	inoculation amount of 6%	734:757	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	50	theme	%	757:757	arg1	proportions					712:722	Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions	641:722	Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1	641:731	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	50	theme	%	757:757	arg1	9:1					636:638	9:1	636:638	9:1	636:638	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	50	theme	%	757:757	arg1	%					757:757	6%	756:757	6%	756:757	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	1	51	theme	agricultural	135:146	arg1	by-product					163:172	an agricultural and industrial by-product	132:172	an agricultural and industrial by-product produced during chrysanthemum harvesting, drying, preservation, and deep processing	132:256	Chrysanthemum waste (CW) is an agricultural and industrial by-product produced during chrysanthemum harvesting, drying, preservation, and deep processing.					
36587550	1	51	theme	agricultural	135:146	arg1	waste					118:122	Chrysanthemum waste	104:122	Chrysanthemum waste (CW)	104:127	Chrysanthemum waste (CW) is an agricultural and industrial by-product produced during chrysanthemum harvesting, drying, preservation, and deep processing.					
36587550	5	52	theme	amino	1207:1211	arg1	composition					1218:1228	the amino acid composition	1203:1228	the amino acid composition of FCW protein	1203:1243	SSF improved the free and hydrolyzed amino acid profiles of FCW, increased the content of flavor amino acids, and improved the amino acid composition of FCW protein.					
36587550	3	53	theme	CW	450:451	arg1	quality					453:459	CW quality	450:459	CW quality for use as an alternative feed ingredient	450:501	This work explored a solid-state fermentation (SSF) strategy to improve CW quality for use as an alternative feed ingredient.					
36587550	4	54	theme	tropicalis + Bacillus	672:692	arg1	proportions					712:722	Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions	641:722	Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1	641:731	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	5	55	theme	acid	1213:1216	arg1	composition					1218:1228	the amino acid composition	1203:1228	the amino acid composition of FCW protein	1203:1243	SSF improved the free and hydrolyzed amino acid profiles of FCW, increased the content of flavor amino acids, and improved the amino acid composition of FCW protein.					
36587550	4	56	theme	detergent	941:949	arg1	fiber					951:955	neutral detergent fiber	933:955	neutral detergent fiber	933:955	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	56	theme	detergent	941:949	arg1	p < 0.01					1006:1013	p < 0.01	1006:1013	p < 0.01	1006:1013	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	4	56	theme	detergent	941:949	arg1	protein					874:880	crude protein	868:880	crude protein	868:880	Orthogonal experiment showed that the optimal conditions for fermented chrysanthemum waste (FCW) were: CW to cornmeal mass ratio of 9:1, Pediococcus cellaris + Candida tropicalis + Bacillus amyloliquefaciens proportions of 2:2:1, inoculation amount of 6%, and fermentation time of 10 d. Compared with the control group, FCW significantly increased the contents of crude protein, ether extract, crude fiber, acid detergent fiber, neutral detergent fiber, ash, calcium, phosphorus, and total flavonoids (p < 0.01), and significantly decreased pH and saponin content (p < 0.01).					
36587550	7	57	theme	processing	1386:1395	arg1	waste					1363:1367	the waste	1359:1367	the waste of chrysanthemum processing	1359:1395	FCW shows potential use as a feed ingredient, and SSF helps reduce the waste of chrysanthemum processing.					
36587550	5	58	theme	flavor	1170:1175	arg1	acids					1183:1187	flavor amino acids	1170:1187	flavor amino acids	1170:1187	SSF improved the free and hydrolyzed amino acid profiles of FCW, increased the content of flavor amino acids, and improved the amino acid composition of FCW protein.					
36587550	2	59	theme	environmental	354:366	arg1	problems					368:375	serious environmental problems	346:375	serious environmental problems	346:375	Although it is nutritious, most CW is discarded, wasting resources and contributing to serious environmental problems.					
36587550	5	60	theme	amino	1177:1181	arg1	acids					1183:1187	flavor amino acids	1170:1187	flavor amino acids	1170:1187	SSF improved the free and hydrolyzed amino acid profiles of FCW, increased the content of flavor amino acids, and improved the amino acid composition of FCW protein.					
36587550	1	61	theme	deep	242:245	arg1	processing					247:256	deep processing	242:256	deep processing	242:256	Chrysanthemum waste (CW) is an agricultural and industrial by-product produced during chrysanthemum harvesting, drying, preservation, and deep processing.					
35164126	5	0	theme	protein	1245:1251	arg1	g					1226:1226	3 g	1224:1226	3 g	1224:1226	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	5	0	theme	protein	1245:1251	arg1	g					1213:1213	4 g	1211:1213	4 g	1211:1213	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	5	0	theme	protein	1245:1251	arg1	g					1218:1218	5 g	1216:1218	5 g	1216:1218	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	4	1	theme	0.47	1072:1075	arg1	MPa					1077:1079	MPa	1077:1079	MPa	1077:1079	The WVP of the edible films varied from 0.90 to 1.62 × 10-11 g.m/Pa.s.m2, the resistance to traction varied from 0.47 MPa to 3.03 MPa and the solubility varied from 51.06% to 87%.					
35164126	6	2	theme	packaging	1763:1771	arg1	materials					1773:1781	synthetic packaging materials	1753:1781	synthetic packaging materials	1753:1781	The results revealed that the optimized formulation of the CEF of G-W-C allowed a good validation of the prediction model and could be applied, in an effective manner, to the food packaging industry, which could help in mitigating the environmental issues associated with synthetic packaging materials.					
35164126	4	3	dep	MPa	1077:1079	arg1	to					1081:1082	to	1081:1082	to	1081:1082	The WVP of the edible films varied from 0.90 to 1.62 × 10-11 g.m/Pa.s.m2, the resistance to traction varied from 0.47 MPa to 3.03 MPa and the solubility varied from 51.06% to 87%.					
35164126	2	4	theme	˃	639:639	arg1	0.90					641:644	R ˃ 0.90	637:644	R ˃ 0.90	637:644	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	2	4	theme	˃	639:639	arg1	fit					632:634	a good fit	625:634	a good fit (R ˃ 0.90)	625:645	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	1	5	theme	optical	297:303	arg1	properties					320:329	optical and mechanical properties	297:329	optical and mechanical properties of composite edible films (CEFs)	297:362	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	6	6	theme	environmental	1716:1728	arg1	issues					1730:1735	the environmental issues	1712:1735	the environmental issues associated with synthetic packaging materials	1712:1781	The results revealed that the optimized formulation of the CEF of G-W-C allowed a good validation of the prediction model and could be applied, in an effective manner, to the food packaging industry, which could help in mitigating the environmental issues associated with synthetic packaging materials.					
35164126	1	7	theme	mechanical	309:318	arg1	properties					320:329	optical and mechanical properties	297:329	optical and mechanical properties of composite edible films (CEFs)	297:362	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	1	8	theme	work	145:148	arg1	aim					133:135	The aim	129:135	The aim of this work	129:148	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	3	9	theme	protein	822:828	arg1	concentrations					790:803	the concentrations	786:803	the concentrations of gelatin, whey protein and chitosan	786:841	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	10	theme	films	952:956	arg1	strength					914:921	strength	914:921	strength	914:921	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	10	theme	films	952:956	arg1	permeability					894:905	the water vapor permeability	878:905	(p ≤ 0.05) the water vapor permeability (WVP)	867:911	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	10	theme	films	952:956	arg1	solubility					927:936	solubility	927:936	solubility	927:936	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	0	11	theme	Whey	102:105	arg1	Optimization					0:11	Optimization	0:11	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin	0:99	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan.					
35164126	0	11	theme	Whey	102:105	arg1	Protein					107:113	Whey Protein	102:113	Whey Protein	102:113	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan.					
35164126	2	12	theme	coefficient	601:611	arg1	values					613:618	the determination coefficient values	583:618	the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*	583:724	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	5	13	theme	chitosan	1257:1264	arg1	g					1226:1226	3 g	1224:1226	3 g	1224:1226	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	5	13	theme	chitosan	1257:1264	arg1	g					1213:1213	4 g	1211:1213	4 g	1211:1213	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	5	13	theme	chitosan	1257:1264	arg1	g					1218:1218	5 g	1216:1218	5 g	1216:1218	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	2	14	theme	first-	520:525	arg1	analysis					564:571	first- and second-order multiple regression analysis	520:571	first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*	520:724	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	6	15	theme	G-W-C	1547:1551	arg1	CEF					1540:1542	the CEF	1536:1542	the CEF of G-W-C	1536:1551	The results revealed that the optimized formulation of the CEF of G-W-C allowed a good validation of the prediction model and could be applied, in an effective manner, to the food packaging industry, which could help in mitigating the environmental issues associated with synthetic packaging materials.					
35164126	6	16	theme	CEF	1540:1542	arg1	formulation					1521:1531	the optimized formulation	1507:1531	the optimized formulation of the CEF of G-W-C	1507:1551	The results revealed that the optimized formulation of the CEF of G-W-C allowed a good validation of the prediction model and could be applied, in an effective manner, to the food packaging industry, which could help in mitigating the environmental issues associated with synthetic packaging materials.					
35164126	2	17	theme	regression	553:562	arg1	analysis					564:571	first- and second-order multiple regression analysis	520:571	first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*	520:724	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	0	18	theme	Properties	53:62	arg1	Optimization					0:11	Optimization	0:11	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin	0:99	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan.					
35164126	0	18	theme	Properties	53:62	arg1	Chitosan					119:126	Chitosan	119:126	Chitosan	119:126	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan.					
35164126	0	18	theme	Properties	53:62	arg1	Protein					107:113	Whey Protein	102:113	Whey Protein	102:113	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan.					
35164126	6	19	theme	synthetic	1753:1761	arg1	materials					1773:1781	synthetic packaging materials	1753:1781	synthetic packaging materials	1753:1781	The results revealed that the optimized formulation of the CEF of G-W-C allowed a good validation of the prediction model and could be applied, in an effective manner, to the food packaging industry, which could help in mitigating the environmental issues associated with synthetic packaging materials.					
35164126	2	20	theme	second-order	531:542	arg1	analysis					564:571	first- and second-order multiple regression analysis	520:571	first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*	520:724	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	6	21	theme	optimized	1511:1519	arg1	formulation					1521:1531	the optimized formulation	1507:1531	the optimized formulation of the CEF of G-W-C	1507:1551	The results revealed that the optimized formulation of the CEF of G-W-C allowed a good validation of the prediction model and could be applied, in an effective manner, to the food packaging industry, which could help in mitigating the environmental issues associated with synthetic packaging materials.					
35164126	4	22	theme	51.06	1124:1128	arg1	%					1129:1129	%	1129:1129	%	1129:1129	The WVP of the edible films varied from 0.90 to 1.62 × 10-11 g.m/Pa.s.m2, the resistance to traction varied from 0.47 MPa to 3.03 MPa and the solubility varied from 51.06% to 87%.					
35164126	0	23	theme	Composite	67:75	arg1	Films					84:88	Composite Edible Films	67:88	Composite Edible Films of Gelatin	67:99	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan.					
35164126	6	24	theme	model	1597:1601	arg1	validation					1568:1577	a good validation	1561:1577	a good validation of the prediction model	1561:1601	The results revealed that the optimized formulation of the CEF of G-W-C allowed a good validation of the prediction model and could be applied, in an effective manner, to the food packaging industry, which could help in mitigating the environmental issues associated with synthetic packaging materials.					
35164126	0	25	theme	Films	84:88	arg1	Properties					53:62	the Physical, Optical and Mechanical Properties	16:62	the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin	16:99	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan.					
35164126	3	26	theme	chitosan	834:841	arg1	concentrations					790:803	the concentrations	786:803	the concentrations of gelatin, whey protein and chitosan	786:841	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	4	27	dep	1.62	1007:1010	arg1	to					1004:1005	to	1004:1005	to	1004:1005	The WVP of the edible films varied from 0.90 to 1.62 × 10-11 g.m/Pa.s.m2, the resistance to traction varied from 0.47 MPa to 3.03 MPa and the solubility varied from 51.06% to 87%.					
35164126	5	28	theme	optimized	1143:1151	arg1	values					1153:1158	The optimized values	1139:1158	The optimized values	1139:1158	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	4	29	theme	edible	974:979	arg1	films					981:985	the edible films	970:985	the edible films	970:985	The WVP of the edible films varied from 0.90 to 1.62 × 10-11 g.m/Pa.s.m2, the resistance to traction varied from 0.47 MPa to 3.03 MPa and the solubility varied from 51.06% to 87%.					
35164126	5	30	theme	continuous	1313:1322	arg1	surface					1340:1346	a smooth, continuous and transparent surface	1303:1346	a smooth, continuous and transparent surface	1303:1346	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	0	31	theme	Gelatin	93:99	arg1	Films					84:88	Composite Edible Films	67:88	Composite Edible Films of Gelatin	67:99	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan.					
35164126	1	32	theme	chitosan	255:262	arg1	concentration					184:196	the concentration	180:196	the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g)	180:278	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	3	33	theme	≤	870:870	arg1	permeability					894:905	the water vapor permeability	878:905	(p ≤ 0.05) the water vapor permeability (WVP)	867:911	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	33	theme	≤	870:870	arg1	p					868:868	p ≤ 0.05	868:875	p ≤ 0.05	868:875	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	2	34	theme	study	495:499	arg1	results					480:486	The results	476:486	The results of the study	476:499	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	5	35	theme	transparent	1328:1338	arg1	surface					1340:1346	a smooth, continuous and transparent surface	1303:1346	a smooth, continuous and transparent surface	1303:1346	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	5	36	with	CEF	1294:1296	arg1	surface					1340:1346	a smooth, continuous and transparent surface	1303:1346	a smooth, continuous and transparent surface	1303:1346	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	5	37	theme	g	1213:1213	arg1	quantity					1199:1206	a quantity	1197:1206	a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively	1197:1278	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	3	38	theme	water	882:886	arg1	strength					914:921	strength	914:921	strength	914:921	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	38	theme	water	882:886	arg1	permeability					894:905	the water vapor permeability	878:905	(p ≤ 0.05) the water vapor permeability (WVP)	867:911	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	38	theme	water	882:886	arg1	p					868:868	p ≤ 0.05	868:875	p ≤ 0.05	868:875	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	38	theme	water	882:886	arg1	solubility					927:936	solubility	927:936	solubility	927:936	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	38	theme	water	882:886	arg1	WVP					908:910	WVP	908:910	WVP	908:910	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	6	39	theme	effective	1631:1639	arg1	manner					1641:1646	an effective manner	1628:1646	an effective manner	1628:1646	The results revealed that the optimized formulation of the CEF of G-W-C allowed a good validation of the prediction model and could be applied, in an effective manner, to the food packaging industry, which could help in mitigating the environmental issues associated with synthetic packaging materials.					
35164126	5	40	theme	smooth	1305:1310	arg1	surface					1340:1346	a smooth, continuous and transparent surface	1303:1346	a smooth, continuous and transparent surface	1303:1346	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	1	41	theme	whey	222:225	arg1	protein					227:233	whey protein	222:233	whey protein (W) (2.5-7.5 g)	222:249	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	1	41	theme	whey	222:225	arg1	g					248:248	2.5-7.5 g	240:248	2.5-7.5 g	240:248	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	1	41	theme	whey	222:225	arg1	W					236:236	W	236:236	W	236:236	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	3	42	theme	linear	742:747	arg1	effect					749:754	The individual linear effect	727:754	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan)	727:842	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	5	43	dep	strength	1423:1430	arg1	resistance					1433:1442	resistance	1433:1442	resistance to traction	1433:1454	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	2	44	theme	*	724:724	arg1	values					698:703	the values	694:703	the values of solubility and b*	694:724	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	4	45	theme	10-11	1014:1018	arg1	g.m/Pa.s.m2					1020:1030	0.90 to 1.62 × 10-11 g.m/Pa.s.m2	999:1030	0.90 to 1.62 × 10-11 g.m/Pa.s.m2	999:1030	The WVP of the edible films varied from 0.90 to 1.62 × 10-11 g.m/Pa.s.m2, the resistance to traction varied from 0.47 MPa to 3.03 MPa and the solubility varied from 51.06% to 87%.					
35164126	1	46	theme	films	351:355	arg1	properties					320:329	optical and mechanical properties	297:329	optical and mechanical properties of composite edible films (CEFs)	297:362	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	2	47	theme	R	637:637	arg1	0.90					641:644	R ˃ 0.90	637:644	R ˃ 0.90	637:644	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	2	47	theme	R	637:637	arg1	fit					632:634	a good fit	625:634	a good fit (R ˃ 0.90)	625:645	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	5	48	theme	lower	1463:1467	arg1	solubility					1469:1478	a lower solubility	1461:1478	a lower solubility	1461:1478	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	3	49	theme	independent	763:773	arg1	variables					775:783	the independent variables	759:783	the independent variables (the concentrations of gelatin, whey protein and chitosan)	759:842	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	5	50	theme	gelatin	1231:1237	arg1	g					1226:1226	3 g	1224:1226	3 g	1224:1226	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	5	50	theme	gelatin	1231:1237	arg1	g					1213:1213	4 g	1211:1213	4 g	1211:1213	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	5	50	theme	gelatin	1231:1237	arg1	g					1218:1218	5 g	1216:1218	5 g	1216:1218	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	1	51	dep	evaluate	157:164	arg1	using					364:368	using	364:368	using the response surface methodology (RSM)	364:407	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	1	51	dep	evaluate	157:164	arg1	optimizing					421:430	optimizing	421:430	optimizing the formulation for the packaging of foods	421:473	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	5	52	theme	whey	1240:1243	arg1	protein					1245:1251	whey protein	1240:1251	whey protein	1240:1251	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	1	53	theme	response	374:381	arg1	RSM					404:406	RSM	404:406	RSM	404:406	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	1	53	theme	response	374:381	arg1	methodology					391:401	the response surface methodology	370:401	the response surface methodology (RSM)	370:407	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	5	54	theme	L	1354:1354	arg1	values					1356:1361	L values	1354:1361	L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility	1354:1478	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	5	55	theme	lower	1402:1406	arg1	WVP					1408:1410	a lower WVP	1400:1410	a lower WVP	1400:1410	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	3	56	theme	edible	945:950	arg1	films					952:956	the edible films	941:956	the edible films	941:956	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	2	57	theme	determination	587:599	arg1	values					613:618	the determination coefficient values	583:618	the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*	583:724	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	5	58	theme	g	1226:1226	arg1	quantity					1199:1206	a quantity	1197:1206	a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively	1197:1278	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	2	59	with	values	613:618	arg1	0.90					641:644	R ˃ 0.90	637:644	R ˃ 0.90	637:644	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	2	59	with	values	613:618	arg1	fit					632:634	a good fit	625:634	a good fit (R ˃ 0.90)	625:645	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	3	60	theme	whey	817:820	arg1	protein					822:828	whey protein	817:828	whey protein	817:828	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	1	61	theme	foods	469:473	arg1	packaging					456:464	the packaging	452:464	the packaging of foods	452:473	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	5	62	theme	light-yellow	1382:1393	arg1	hue					1395:1397	a light-yellow hue	1380:1397	a light-yellow hue	1380:1397	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	3	63	theme	gelatin	808:814	arg1	concentrations					790:803	the concentrations	786:803	the concentrations of gelatin, whey protein and chitosan	786:841	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	1	64	theme	composite	334:342	arg1	CEFs					358:361	CEFs	358:361	CEFs	358:361	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	1	64	theme	composite	334:342	arg1	films					351:355	composite edible films	334:355	composite edible films (CEFs)	334:362	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	3	65	dep	variables	775:783	arg1	concentrations					790:803	the concentrations	786:803	the concentrations of gelatin, whey protein and chitosan	786:841	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	4	66	theme	3.03	1084:1087	arg1	MPa					1077:1079	MPa	1077:1079	MPa	1077:1079	The WVP of the edible films varied from 0.90 to 1.62 × 10-11 g.m/Pa.s.m2, the resistance to traction varied from 0.47 MPa to 3.03 MPa and the solubility varied from 51.06% to 87%.					
35164126	5	67	theme	maximum	1415:1421	arg1	strength					1423:1430	a maximum strength	1413:1430	a maximum strength (resistance to traction)	1413:1455	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	4	68	theme	87	1134:1135	arg1	%					1129:1129	%	1129:1129	%	1129:1129	The WVP of the edible films varied from 0.90 to 1.62 × 10-11 g.m/Pa.s.m2, the resistance to traction varied from 0.47 MPa to 3.03 MPa and the solubility varied from 51.06% to 87%.					
35164126	2	69	theme	multiple	544:551	arg1	analysis					564:571	first- and second-order multiple regression analysis	520:571	first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*	520:724	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	0	70	theme	Edible	77:82	arg1	Films					84:88	Composite Edible Films	67:88	Composite Edible Films of Gelatin	67:99	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan.					
35164126	6	71	theme	food	1656:1659	arg1	industry					1671:1678	the food packaging industry	1652:1678	the food packaging industry	1652:1678	The results revealed that the optimized formulation of the CEF of G-W-C allowed a good validation of the prediction model and could be applied, in an effective manner, to the food packaging industry, which could help in mitigating the environmental issues associated with synthetic packaging materials.					
35164126	6	72	theme	packaging	1661:1669	arg1	industry					1671:1678	the food packaging industry	1652:1678	the food packaging industry	1652:1678	The results revealed that the optimized formulation of the CEF of G-W-C allowed a good validation of the prediction model and could be applied, in an effective manner, to the food packaging industry, which could help in mitigating the environmental issues associated with synthetic packaging materials.					
35164126	4	73	theme	films	981:985	arg1	WVP					963:965	The WVP	959:965	The WVP of the edible films	959:985	The WVP of the edible films varied from 0.90 to 1.62 × 10-11 g.m/Pa.s.m2, the resistance to traction varied from 0.47 MPa to 3.03 MPa and the solubility varied from 51.06% to 87%.					
35164126	6	74	theme	prediction	1586:1595	arg1	model					1597:1601	the prediction model	1582:1601	the prediction model	1582:1601	The results revealed that the optimized formulation of the CEF of G-W-C allowed a good validation of the prediction model and could be applied, in an effective manner, to the food packaging industry, which could help in mitigating the environmental issues associated with synthetic packaging materials.					
35164126	1	75	theme	surface	383:389	arg1	RSM					404:406	RSM	404:406	RSM	404:406	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	1	75	theme	surface	383:389	arg1	methodology					391:401	the response surface methodology	370:401	the response surface methodology (RSM)	370:407	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	2	76	theme	solubility	708:717	arg1	values					698:703	the values	694:703	the values of solubility and b*	694:724	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	6	77	theme	good	1563:1566	arg1	validation					1568:1577	a good validation	1561:1577	a good validation of the prediction model	1561:1601	The results revealed that the optimized formulation of the CEF of G-W-C allowed a good validation of the prediction model and could be applied, in an effective manner, to the food packaging industry, which could help in mitigating the environmental issues associated with synthetic packaging materials.					
35164126	0	78	theme	Physical	20:27	arg1	Properties					53:62	the Physical, Optical and Mechanical Properties	16:62	the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin	16:99	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan.					
35164126	4	79	theme	×	1012:1012	arg1	g.m/Pa.s.m2					1020:1030	0.90 to 1.62 × 10-11 g.m/Pa.s.m2	999:1030	0.90 to 1.62 × 10-11 g.m/Pa.s.m2	999:1030	The WVP of the edible films varied from 0.90 to 1.62 × 10-11 g.m/Pa.s.m2, the resistance to traction varied from 0.47 MPa to 3.03 MPa and the solubility varied from 51.06% to 87%.					
35164126	0	80	theme	Optical	30:36	arg1	Properties					53:62	the Physical, Optical and Mechanical Properties	16:62	the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin	16:99	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan.					
35164126	4	81	dep	%	1129:1129	arg1	to					1131:1132	to	1131:1132	to	1131:1132	The WVP of the edible films varied from 0.90 to 1.62 × 10-11 g.m/Pa.s.m2, the resistance to traction varied from 0.47 MPa to 3.03 MPa and the solubility varied from 51.06% to 87%.					
35164126	5	82	theme	g	1218:1218	arg1	quantity					1199:1206	a quantity	1197:1206	a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively	1197:1278	The optimized values indicated that the CEF prepared with a quantity of 4 g, 5 g and 3 g of gelatin, whey protein and chitosan, respectively, provided the CEF with a smooth, continuous and transparent surface, with L values that resulted in a light-yellow hue, a lower WVP, a maximum strength (resistance to traction) and a lower solubility.					
35164126	1	83	theme	edible	344:349	arg1	CEFs					358:361	CEFs	358:361	CEFs	358:361	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	1	83	theme	edible	344:349	arg1	films					351:355	composite edible films	334:355	composite edible films (CEFs)	334:362	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	0	84	theme	Mechanical	42:51	arg1	Properties					53:62	the Physical, Optical and Mechanical Properties	16:62	the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin	16:99	Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan.					
35164126	1	85	theme	protein	227:233	arg1	concentration					184:196	the concentration	180:196	the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g)	180:278	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
35164126	2	86	theme	b	723:723	arg1	*					724:724	b*	723:724	b*	723:724	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	3	87	theme	vapor	888:892	arg1	strength					914:921	strength	914:921	strength	914:921	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	87	theme	vapor	888:892	arg1	permeability					894:905	the water vapor permeability	878:905	(p ≤ 0.05) the water vapor permeability (WVP)	867:911	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	87	theme	vapor	888:892	arg1	p					868:868	p ≤ 0.05	868:875	p ≤ 0.05	868:875	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	87	theme	vapor	888:892	arg1	solubility					927:936	solubility	927:936	solubility	927:936	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	87	theme	vapor	888:892	arg1	WVP					908:910	WVP	908:910	WVP	908:910	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	3	88	theme	individual	731:740	arg1	effect					749:754	The individual linear effect	727:754	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan)	727:842	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	2	89	theme	response	663:670	arg1	variables					672:680	the response variables	659:680	the response variables	659:680	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	3	90	theme	variables	775:783	arg1	effect					749:754	The individual linear effect	727:754	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan)	727:842	The individual linear effect of the independent variables (the concentrations of gelatin, whey protein and chitosan) significantly affected (p ≤ 0.05) the water vapor permeability (WVP), strength and solubility of the edible films.					
35164126	2	91	theme	good	627:630	arg1	0.90					641:644	R ˃ 0.90	637:644	R ˃ 0.90	637:644	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	2	91	theme	good	627:630	arg1	fit					632:634	a good fit	625:634	a good fit (R ˃ 0.90)	625:645	The results of the study were evaluated via first- and second-order multiple regression analysis to obtain the determination coefficient values with a good fit (R ˃ 0.90) for each of the response variables, except for the values of solubility and b*.					
35164126	1	92	theme	gelatin	201:207	arg1	concentration					184:196	the concentration	180:196	the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g)	180:278	The aim of this work was to evaluate the effect of the concentration of gelatin (G) (3-6 g), whey protein (W) (2.5-7.5 g) and chitosan (C) (0.5-2.5 g) on the physical, optical and mechanical properties of composite edible films (CEFs) using the response surface methodology (RSM), as well as optimizing the formulation for the packaging of foods.					
37085526	4	0	theme	fecal/serum	983:993	arg1	sequencing					1018:1027	16 S rRNA sequencing	1008:1027	16 S rRNA sequencing	1008:1027	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	4	0	theme	fecal/serum	983:993	arg1	metabolomes					995:1005	fecal/serum metabolomes	983:1005	fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling)	983:1052	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	8	1	theme	butyric	1540:1546	arg1	concentrations					1567:1580	increased serum butyric and propionic acid concentrations	1524:1580	increased serum butyric and propionic acid concentrations	1524:1580	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37085526	5	2	theme	dietary	1126:1132	arg1	intervention					1152:1163	dietary inulin (10 g/day) intervention	1126:1163	dietary inulin (10 g/day) intervention	1126:1163	Subsequently, 27 DM were recruited for a predictive study: 3 months of dietary inulin (10 g/day) intervention.					
37085526	8	3	theme	propionic	1552:1560	arg1	concentrations					1567:1580	increased serum butyric and propionic acid concentrations	1524:1580	increased serum butyric and propionic acid concentrations	1524:1580	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37085526	2	4	theme	targeted	271:278	arg1	interventions					288:300	targeted dietary interventions	271:300	targeted dietary interventions	271:300	However, targeted dietary interventions are limited by the highly variable inter-individual response.					
37085526	9	5	theme	improved	1587:1594	arg1	dependent					1639:1647	dependent	1639:1647	dependent	1639:1647	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	5	theme	improved	1587:1594	arg1	outcomes					1605:1612	The improved glycemic outcomes	1583:1612	The improved glycemic outcomes of inulin treatment	1583:1632	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	6	theme	Eubacterium	1795:1805	arg1	taxa					1774:1777	certain bacterial taxa	1756:1777	certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium)	1756:1893	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	6	theme	Eubacterium	1795:1805	arg1	group					1813:1817	Eubacterium halii group	1795:1817	Eubacterium halii group	1795:1817	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	3	7	theme	baseline	656:663	arg1	signature					670:678	baseline MIME signature	656:678	baseline MIME signature	656:678	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	9	8	dep	taxa	1774:1777	arg1	Dialister					1858:1866	Dialister	1858:1866	Dialister	1858:1866	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	8	dep	taxa	1774:1777	arg1	Blautia					1786:1792	Blautia	1786:1792	Blautia	1786:1792	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	8	dep	taxa	1774:1777	arg1	Phascolarctobacterium					1872:1892	Phascolarctobacterium	1872:1892	Phascolarctobacterium	1872:1892	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	8	dep	taxa	1774:1777	arg1	Ruminiclostridium					1839:1855	Ruminiclostridium	1839:1855	Ruminiclostridium	1839:1855	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	8	dep	taxa	1774:1777	arg1	taxa					1774:1777	certain bacterial taxa	1756:1777	certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium)	1756:1893	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	8	dep	taxa	1774:1777	arg1	group					1813:1817	Eubacterium halii group	1795:1817	Eubacterium halii group	1795:1817	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	1	9	theme	metabolic	173:181	arg1	performance					183:193	The metabolic performance	169:193	AIM The metabolic performance of the gut microbiota	165:215	AIM The metabolic performance of the gut microbiota contributes to the onset of type 2 diabetes.					
37085526	9	10	theme	better	1652:1657	arg1	status					1677:1682	better baseline glycemic status	1652:1682	better baseline glycemic status	1652:1682	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	6	11	theme	RESULTS	1166:1172	arg1	composition					1179:1189	RESULTS MIME composition	1166:1189	RESULTS MIME composition	1166:1189	RESULTS MIME composition was different between groups.					
37085526	11	12	from	differences	2281:2291	arg1	status					2314:2319	baseline glycemic status	2296:2319	baseline glycemic status	2296:2319	The large inter-individual variability in the metabolic effects of dietary inulin was explained by differences in baseline glycemic status and MIME signatures.					
37085526	11	12	from	differences	2281:2291	arg1	signatures					2330:2339	MIME signatures	2325:2339	MIME signatures	2325:2339	The large inter-individual variability in the metabolic effects of dietary inulin was explained by differences in baseline glycemic status and MIME signatures.					
37085526	10	13	theme	patterns	2139:2146	arg1	obesity					2087:2093	obesity	2087:2093	obesity	2087:2093	CONCLUSION We demonstrated that obesity is a stronger determinant of different MIME patterns than impaired glucose metabolism.					
37085526	10	13	theme	patterns	2139:2146	arg1	determinant					2109:2119	a stronger determinant	2098:2119	a stronger determinant of different MIME patterns than impaired glucose metabolism	2098:2179	CONCLUSION We demonstrated that obesity is a stronger determinant of different MIME patterns than impaired glucose metabolism.					
37085526	9	14	theme	inulin	1617:1622	arg1	treatment					1624:1632	inulin treatment	1617:1632	inulin treatment	1617:1632	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	4	15	with	patients	728:735	arg1	pre/diabetes					758:769	newly diagnosed pre/diabetes	742:769	newly diagnosed pre/diabetes	742:769	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	3	16	theme	MIME	530:533	arg1	patterns					535:542	specific MIME patterns	521:542	specific MIME patterns	521:542	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	1	17	theme	gut	202:204	arg1	microbiota					206:215	the gut microbiota	198:215	the gut microbiota	198:215	AIM The metabolic performance of the gut microbiota contributes to the onset of type 2 diabetes.					
37085526	11	18	theme	glycemic	2305:2312	arg1	status					2314:2319	baseline glycemic status	2296:2319	baseline glycemic status	2296:2319	The large inter-individual variability in the metabolic effects of dietary inulin was explained by differences in baseline glycemic status and MIME signatures.					
37085526	11	19	theme	inulin	2257:2262	arg1	effects					2238:2244	the metabolic effects	2224:2244	the metabolic effects of dietary inulin	2224:2262	The large inter-individual variability in the metabolic effects of dietary inulin was explained by differences in baseline glycemic status and MIME signatures.					
37085526	4	20	theme	lean	840:843	arg1	volunteers					850:859	32 healthy lean (LH) volunteers	829:859	32 healthy lean (LH) volunteers	829:859	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	8	21	theme	overall	1369:1375	arg1	improvement					1377:1387	an overall improvement	1366:1387	an overall improvement in glycemic indices	1366:1407	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37085526	7	22	theme	spectrum	1292:1299	arg1	poles					1269:1273	opposite poles	1260:1273	opposite poles of the abundance spectrum	1260:1299	While the DM and LH groups represented opposite poles of the abundance spectrum, OB was closer to DM.					
37085526	4	23	theme	LH	846:847	arg1	volunteers					850:859	32 healthy lean (LH) volunteers	829:859	32 healthy lean (LH) volunteers	829:859	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	0	24	theme	interventional	143:156	arg1	trial					158:162	an interventional trial	140:162	an interventional trial	140:162	Multi-omics signatures in new-onset diabetes predict metabolic response to dietary inulin: findings from an observational study followed by an interventional trial.					
37085526	9	25	theme	baseline	1659:1666	arg1	status					1677:1682	better baseline glycemic status	1652:1682	better baseline glycemic status	1652:1682	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	26	theme	serum	1896:1900	arg1	concentrations					1902:1915	serum concentrations	1896:1915	serum concentrations of branched-chain amino acid derivatives and asparagine	1896:1971	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	10	27	theme	stronger	2100:2107	arg1	obesity					2087:2093	obesity	2087:2093	obesity	2087:2093	CONCLUSION We demonstrated that obesity is a stronger determinant of different MIME patterns than impaired glucose metabolism.					
37085526	10	27	theme	stronger	2100:2107	arg1	determinant					2109:2119	a stronger determinant	2098:2119	a stronger determinant of different MIME patterns than impaired glucose metabolism	2098:2179	CONCLUSION We demonstrated that obesity is a stronger determinant of different MIME patterns than impaired glucose metabolism.					
37085526	5	28	theme	inulin	1134:1139	arg1	intervention					1152:1163	dietary inulin (10 g/day) intervention	1126:1163	dietary inulin (10 g/day) intervention	1126:1163	Subsequently, 27 DM were recruited for a predictive study: 3 months of dietary inulin (10 g/day) intervention.					
37085526	3	29	theme	dietary	588:594	arg1	inulin					596:601	dietary inulin	588:601	dietary inulin	588:601	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	12	30	theme	diagnosed	2439:2447	arg1	diabetes					2449:2456	newly diagnosed diabetes	2433:2456	newly diagnosed diabetes	2433:2456	These could be further validated to personalize nutritional interventions in patients with newly diagnosed diabetes.					
37085526	9	31	theme	amino	1935:1939	arg1	derivatives					1946:1956	branched-chain amino acid derivatives	1920:1956	branched-chain amino acid derivatives	1920:1956	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	32	theme	related	1698:1704	arg1	variables					1688:1696	variables	1688:1696	variables related to the gut microbiota	1688:1726	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	32	theme	related	1698:1704	arg1	abundance					1743:1751	the abundance	1739:1751	the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds	1739:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	4	33	with	overweight/obese	802:817	arg1	pre/diabetes					758:769	newly diagnosed pre/diabetes	742:769	newly diagnosed pre/diabetes	742:769	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	9	34	theme	derivatives	1946:1956	arg1	Dialister					1858:1866	Dialister	1858:1866	Dialister	1858:1866	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	34	theme	derivatives	1946:1956	arg1	concentrations					1902:1915	serum concentrations	1896:1915	serum concentrations of branched-chain amino acid derivatives and asparagine	1896:1971	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	34	theme	derivatives	1946:1956	arg1	Blautia					1786:1792	Blautia	1786:1792	Blautia	1786:1792	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	34	theme	derivatives	1946:1956	arg1	Phascolarctobacterium					1872:1892	Phascolarctobacterium	1872:1892	Phascolarctobacterium	1872:1892	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	34	theme	derivatives	1946:1956	arg1	Ruminiclostridium					1839:1855	Ruminiclostridium	1839:1855	Ruminiclostridium	1839:1855	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	34	theme	derivatives	1946:1956	arg1	concentrations					1984:1997	fecal concentrations	1978:1997	fecal concentrations of indole and several other volatile organic compounds	1978:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	34	theme	derivatives	1946:1956	arg1	taxa					1774:1777	certain bacterial taxa	1756:1777	certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium)	1756:1893	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	34	theme	derivatives	1946:1956	arg1	group					1813:1817	Eubacterium halii group	1795:1817	Eubacterium halii group	1795:1817	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	10	35	theme	impaired	2153:2160	arg1	metabolism					2170:2179	impaired glucose metabolism	2153:2179	impaired glucose metabolism	2153:2179	CONCLUSION We demonstrated that obesity is a stronger determinant of different MIME patterns than impaired glucose metabolism.					
37085526	9	36	theme	gut	1713:1715	arg1	microbiota					1717:1726	the gut microbiota	1709:1726	the gut microbiota	1709:1726	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	37	theme	asparagine	1962:1971	arg1	Dialister					1858:1866	Dialister	1858:1866	Dialister	1858:1866	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	37	theme	asparagine	1962:1971	arg1	concentrations					1902:1915	serum concentrations	1896:1915	serum concentrations of branched-chain amino acid derivatives and asparagine	1896:1971	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	37	theme	asparagine	1962:1971	arg1	Blautia					1786:1792	Blautia	1786:1792	Blautia	1786:1792	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	37	theme	asparagine	1962:1971	arg1	Phascolarctobacterium					1872:1892	Phascolarctobacterium	1872:1892	Phascolarctobacterium	1872:1892	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	37	theme	asparagine	1962:1971	arg1	Ruminiclostridium					1839:1855	Ruminiclostridium	1839:1855	Ruminiclostridium	1839:1855	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	37	theme	asparagine	1962:1971	arg1	concentrations					1984:1997	fecal concentrations	1978:1997	fecal concentrations of indole and several other volatile organic compounds	1978:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	37	theme	asparagine	1962:1971	arg1	taxa					1774:1777	certain bacterial taxa	1756:1777	certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium)	1756:1893	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	37	theme	asparagine	1962:1971	arg1	group					1813:1817	Eubacterium halii group	1795:1817	Eubacterium halii group	1795:1817	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	3	38	theme	microbiome	424:433	arg1	composition					393:403	the composition	389:403	the composition of the complex gut microbiome	389:433	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	3	38	theme	microbiome	424:433	arg1	MIME					451:454	MIME	451:454	MIME	451:454	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	3	38	theme	microbiome	424:433	arg1	metabolome					439:448	metabolome	439:448	metabolome (MIME)	439:455	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	4	39	with	volunteers	850:859	arg1	pre/diabetes					758:769	newly diagnosed pre/diabetes	742:769	newly diagnosed pre/diabetes	742:769	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	7	40	theme	opposite	1260:1267	arg1	poles					1269:1273	opposite poles	1260:1273	opposite poles of the abundance spectrum	1260:1299	While the DM and LH groups represented opposite poles of the abundance spectrum, OB was closer to DM.					
37085526	0	41	theme	dietary	75:81	arg1	inulin					83:88	dietary inulin	75:88	dietary inulin	75:88	Multi-omics signatures in new-onset diabetes predict metabolic response to dietary inulin: findings from an observational study followed by an interventional trial.					
37085526	3	42	theme	complex	412:418	arg1	microbiome					424:433	the complex gut microbiome	408:433	the complex gut microbiome	408:433	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	0	43	theme	Multi-omics	0:10	arg1	signatures					12:21	Multi-omics signatures	0:21	Multi-omics signatures in new-onset diabetes	0:43	Multi-omics signatures in new-onset diabetes predict metabolic response to dietary inulin: findings from an observational study followed by an interventional trial.					
37085526	4	44	dep	sequencing	1018:1027	arg1	profiling					1043:1051	metabolomics profiling	1030:1051	metabolomics profiling	1030:1051	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	9	45	theme	concentrations	1984:1997	arg1	abundance					1743:1751	the abundance	1739:1751	the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds	1739:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	4	46	theme	METHOD	710:715	arg1	patients					728:735	METHOD Forty-nine patients	710:735	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM)	710:774	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	4	46	theme	METHOD	710:715	arg1	DM					772:773	DM	772:773	DM	772:773	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	4	47	theme	dietary	947:953	arg1	intake					955:960	dietary intake	947:960	dietary intake	947:960	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	9	48	theme	indole	2002:2007	arg1	compounds					2044:2052	indole and several other volatile organic compounds	2002:2052	compounds	2044:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	8	49	with	variable	1439:1446	arg1	shift					1456:1460	a shift	1454:1460	a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations	1454:1580	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37085526	9	50	theme	bacterial	1764:1772	arg1	Dialister					1858:1866	Dialister	1858:1866	Dialister	1858:1866	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	50	theme	bacterial	1764:1772	arg1	Blautia					1786:1792	Blautia	1786:1792	Blautia	1786:1792	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	50	theme	bacterial	1764:1772	arg1	Phascolarctobacterium					1872:1892	Phascolarctobacterium	1872:1892	Phascolarctobacterium	1872:1892	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	50	theme	bacterial	1764:1772	arg1	Ruminiclostridium					1839:1855	Ruminiclostridium	1839:1855	Ruminiclostridium	1839:1855	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	50	theme	bacterial	1764:1772	arg1	taxa					1774:1777	certain bacterial taxa	1756:1777	certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium)	1756:1893	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	50	theme	bacterial	1764:1772	arg1	group					1813:1817	Eubacterium halii group	1795:1817	Eubacterium halii group	1795:1817	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	51	theme	several	2013:2019	arg1	compounds					2044:2052	indole and several other volatile organic compounds	2002:2052	compounds	2044:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	3	52	theme	metabolic	471:479	arg1	lean-obese-diabetes					490:508	lean-obese-diabetes	490:508	lean-obese-diabetes	490:508	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	3	52	theme	metabolic	471:479	arg1	spectra					481:487	metabolic spectra	471:487	metabolic spectra (lean-obese-diabetes)	471:509	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	0	53	theme	metabolic	53:61	arg1	response					63:70	metabolic response	53:70	metabolic response to dietary inulin	53:88	Multi-omics signatures in new-onset diabetes predict metabolic response to dietary inulin: findings from an observational study followed by an interventional trial.					
37085526	9	54	theme	glycemic	1596:1603	arg1	dependent					1639:1647	dependent	1639:1647	dependent	1639:1647	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	54	theme	glycemic	1596:1603	arg1	outcomes					1605:1612	The improved glycemic outcomes	1583:1612	The improved glycemic outcomes of inulin treatment	1583:1632	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	55	theme	volatile	2027:2034	arg1	compounds					2044:2052	indole and several other volatile organic compounds	2002:2052	compounds	2044:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	8	56	theme	acid	1562:1565	arg1	concentrations					1567:1580	increased serum butyric and propionic acid concentrations	1524:1580	increased serum butyric and propionic acid concentrations	1524:1580	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37085526	5	57	theme	10 g/day	1142:1149	arg1	intervention					1152:1163	dietary inulin (10 g/day) intervention	1126:1163	dietary inulin (10 g/day) intervention	1126:1163	Subsequently, 27 DM were recruited for a predictive study: 3 months of dietary inulin (10 g/day) intervention.					
37085526	11	58	theme	MIME	2325:2328	arg1	signatures					2330:2339	MIME signatures	2325:2339	MIME signatures	2325:2339	The large inter-individual variability in the metabolic effects of dietary inulin was explained by differences in baseline glycemic status and MIME signatures.					
37085526	10	59	dep	CONCLUSION	2055:2064	arg1	demonstrated					2069:2080	demonstrated	2069:2080	demonstrated that obesity is a stronger determinant of different MIME patterns than impaired glucose metabolism	2069:2179	CONCLUSION We demonstrated that obesity is a stronger determinant of different MIME patterns than impaired glucose metabolism.					
37085526	4	60	theme	metabolomics	1030:1041	arg1	profiling					1043:1051	metabolomics profiling	1030:1051	metabolomics profiling	1030:1051	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	8	61	theme	favorable	1502:1510	arg1	profile					1512:1518	a more favorable profile	1495:1518	a more favorable profile	1495:1518	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37085526	9	62	theme	compounds	2044:2052	arg1	Dialister					1858:1866	Dialister	1858:1866	Dialister	1858:1866	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	62	theme	compounds	2044:2052	arg1	concentrations					1902:1915	serum concentrations	1896:1915	serum concentrations of branched-chain amino acid derivatives and asparagine	1896:1971	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	62	theme	compounds	2044:2052	arg1	Blautia					1786:1792	Blautia	1786:1792	Blautia	1786:1792	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	62	theme	compounds	2044:2052	arg1	Phascolarctobacterium					1872:1892	Phascolarctobacterium	1872:1892	Phascolarctobacterium	1872:1892	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	62	theme	compounds	2044:2052	arg1	Ruminiclostridium					1839:1855	Ruminiclostridium	1839:1855	Ruminiclostridium	1839:1855	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	62	theme	compounds	2044:2052	arg1	concentrations					1984:1997	fecal concentrations	1978:1997	fecal concentrations of indole and several other volatile organic compounds	1978:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	62	theme	compounds	2044:2052	arg1	taxa					1774:1777	certain bacterial taxa	1756:1777	certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium)	1756:1893	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	62	theme	compounds	2044:2052	arg1	group					1813:1817	Eubacterium halii group	1795:1817	Eubacterium halii group	1795:1817	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	5	63	theme	intervention	1152:1163	arg1	months					1116:1121	3 months	1114:1121	3 months of dietary inulin (10 g/day) intervention	1114:1163	Subsequently, 27 DM were recruited for a predictive study: 3 months of dietary inulin (10 g/day) intervention.					
37085526	0	64	from	study	122:126	arg1	findings					91:98	findings	91:98	findings from an observational study followed by an interventional trial	91:162	Multi-omics signatures in new-onset diabetes predict metabolic response to dietary inulin: findings from an observational study followed by an interventional trial.					
37085526	1	65	theme	type	245:248	arg1	diabetes					252:259	type 2 diabetes	245:259	type 2 diabetes	245:259	AIM The metabolic performance of the gut microbiota contributes to the onset of type 2 diabetes.					
37085526	4	66	theme	16 S	1008:1011	arg1	sequencing					1018:1027	16 S rRNA sequencing	1008:1027	16 S rRNA sequencing	1008:1027	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	4	66	theme	16 S	1008:1011	arg1	metabolomes					995:1005	fecal/serum metabolomes	983:1005	fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling)	983:1052	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	8	67	theme	serum	1534:1538	arg1	concentrations					1567:1580	increased serum butyric and propionic acid concentrations	1524:1580	increased serum butyric and propionic acid concentrations	1524:1580	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37085526	8	68	from	shift	1456:1460	arg1	composition					1476:1486	microbiome composition	1465:1486	microbiome composition	1465:1486	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37085526	2	69	theme	dietary	280:286	arg1	interventions					288:300	targeted dietary interventions	271:300	targeted dietary interventions	271:300	However, targeted dietary interventions are limited by the highly variable inter-individual response.					
37085526	10	70	theme	MIME	2134:2137	arg1	patterns					2139:2146	different MIME patterns	2124:2146	different MIME patterns	2124:2146	CONCLUSION We demonstrated that obesity is a stronger determinant of different MIME patterns than impaired glucose metabolism.					
37085526	1	71	theme	diabetes	252:259	arg1	onset					236:240	the onset	232:240	the onset of type 2 diabetes	232:259	AIM The metabolic performance of the gut microbiota contributes to the onset of type 2 diabetes.					
37085526	11	72	theme	metabolic	2228:2236	arg1	effects					2238:2244	the metabolic effects	2224:2244	the metabolic effects of dietary inulin	2224:2262	The large inter-individual variability in the metabolic effects of dietary inulin was explained by differences in baseline glycemic status and MIME signatures.					
37085526	2	73	theme	inter-individual	337:352	arg1	response					354:361	the highly variable inter-individual response	317:361	the highly variable inter-individual response	317:361	However, targeted dietary interventions are limited by the highly variable inter-individual response.					
37085526	4	74	theme	healthy	794:800	arg1	OB					820:821	OB	820:821	OB	820:821	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	4	74	theme	healthy	794:800	arg1	overweight/obese					802:817	66 metabolically healthy overweight/obese	777:817	66 metabolically healthy overweight/obese (OB)	777:822	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	3	75	theme	MIME	665:668	arg1	signature					670:678	baseline MIME signature	656:678	baseline MIME signature	656:678	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	6	76	theme	MIME	1174:1177	arg1	composition					1179:1189	RESULTS MIME composition	1166:1189	RESULTS MIME composition	1166:1189	RESULTS MIME composition was different between groups.					
37085526	3	77	theme	specific	521:528	arg1	patterns					535:542	specific MIME patterns	521:542	specific MIME patterns	521:542	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	9	78	theme	treatment	1624:1632	arg1	dependent					1639:1647	dependent	1639:1647	dependent	1639:1647	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	78	theme	treatment	1624:1632	arg1	outcomes					1605:1612	The improved glycemic outcomes	1583:1612	The improved glycemic outcomes of inulin treatment	1583:1632	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	11	79	theme	dietary	2249:2255	arg1	inulin					2257:2262	dietary inulin	2249:2262	dietary inulin	2249:2262	The large inter-individual variability in the metabolic effects of dietary inulin was explained by differences in baseline glycemic status and MIME signatures.					
37085526	3	80	theme	differential	562:573	arg1	responses					575:583	the differential responses	558:583	the differential responses to dietary inulin	558:601	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	1	81	theme	microbiota	206:215	arg1	AIM					165:167	AIM	165:167	AIM The metabolic performance of the gut microbiota	165:215	AIM The metabolic performance of the gut microbiota contributes to the onset of type 2 diabetes.					
37085526	10	82	theme	different	2124:2132	arg1	patterns					2139:2146	different MIME patterns	2124:2146	different MIME patterns	2124:2146	CONCLUSION We demonstrated that obesity is a stronger determinant of different MIME patterns than impaired glucose metabolism.					
37085526	4	83	theme	healthy	832:838	arg1	volunteers					850:859	32 healthy lean (LH) volunteers	829:859	32 healthy lean (LH) volunteers	829:859	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	8	84	theme	glycemic	1392:1399	arg1	indices					1401:1407	glycemic indices	1392:1407	glycemic indices	1392:1407	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37085526	9	85	theme	glycemic	1668:1675	arg1	status					1677:1682	better baseline glycemic status	1652:1682	better baseline glycemic status	1652:1682	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	7	86	theme	abundance	1282:1290	arg1	spectrum					1292:1299	the abundance spectrum	1278:1299	the abundance spectrum	1278:1299	While the DM and LH groups represented opposite poles of the abundance spectrum, OB was closer to DM.					
37085526	9	87	theme	concentrations	1902:1915	arg1	abundance					1743:1751	the abundance	1739:1751	the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds	1739:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	8	88	from	improvement	1377:1387	arg1	indices					1401:1407	glycemic indices	1392:1407	glycemic indices	1392:1407	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37085526	11	89	theme	baseline	2296:2303	arg1	status					2314:2319	baseline glycemic status	2296:2319	baseline glycemic status	2296:2319	The large inter-individual variability in the metabolic effects of dietary inulin was explained by differences in baseline glycemic status and MIME signatures.					
37085526	9	90	theme	branched-chain	1920:1933	arg1	derivatives					1946:1956	branched-chain amino acid derivatives	1920:1956	branched-chain amino acid derivatives	1920:1956	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	3	91	theme	clinical	684:691	arg1	characteristics					693:707	clinical characteristics	684:707	clinical characteristics	684:707	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	4	92	theme	clinical	927:934	arg1	variables					936:944	clinical variables	927:944	clinical variables	927:944	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	0	93	from	signatures	12:21	arg1	diabetes					36:43	new-onset diabetes	26:43	new-onset diabetes	26:43	Multi-omics signatures in new-onset diabetes predict metabolic response to dietary inulin: findings from an observational study followed by an interventional trial.					
37085526	9	94	theme	acid	1941:1944	arg1	derivatives					1946:1956	branched-chain amino acid derivatives	1920:1956	branched-chain amino acid derivatives	1920:1956	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	4	95	theme	diagnosed	748:756	arg1	pre/diabetes					758:769	newly diagnosed pre/diabetes	742:769	newly diagnosed pre/diabetes	742:769	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	9	96	dep	Blautia	1786:1792	arg1	i.e.					1780:1783	i.e.	1780:1783	i.e.	1780:1783	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	3	97	dep	differ	457:462	arg1	predicted					637:645	predicted	637:645	can be predicted based on baseline MIME signature and clinical characteristics	630:707	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	3	97	dep	differ	457:462	arg1	2					513:513	2	513:513	2	513:513	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	3	97	dep	differ	457:462	arg1	that					612:615	that	612:615	that	612:615	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	3	97	dep	differ	457:462	arg1	explain					550:556	explain	550:556	could explain the differential responses to dietary inulin	544:601	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	3	97	dep	differ	457:462	arg1	that					516:519	that	516:519	that	516:519	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	3	97	dep	differ	457:462	arg1	1					381:381	1	381:381	1	381:381	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	12	98	with	patients	2419:2426	arg1	diabetes					2449:2456	newly diagnosed diabetes	2433:2456	newly diagnosed diabetes	2433:2456	These could be further validated to personalize nutritional interventions in patients with newly diagnosed diabetes.					
37085526	4	99	theme	Forty-nine	717:726	arg1	patients					728:735	METHOD Forty-nine patients	710:735	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM)	710:774	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	4	99	theme	Forty-nine	717:726	arg1	DM					772:773	DM	772:773	DM	772:773	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	0	100	theme	observational	108:120	arg1	study					122:126	an observational study	105:126	an observational study followed by an interventional trial	105:162	Multi-omics signatures in new-onset diabetes predict metabolic response to dietary inulin: findings from an observational study followed by an interventional trial.					
37085526	4	101	theme	cross-sectional	880:894	arg1	study					909:913	a cross-sectional case-control study	878:913	a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling)	878:1052	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	4	102	theme	case-control	896:907	arg1	study					909:913	a cross-sectional case-control study	878:913	a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling)	878:1052	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	7	103	theme	LH	1238:1239	arg1	groups					1241:1246	the DM and LH groups	1227:1246	the DM and LH groups	1227:1246	While the DM and LH groups represented opposite poles of the abundance spectrum, OB was closer to DM.					
37085526	4	104	theme	gut	963:965	arg1	microbiome					967:976	gut microbiome	963:976	gut microbiome	963:976	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	9	105	theme	fecal	1978:1982	arg1	concentrations					1984:1997	fecal concentrations	1978:1997	fecal concentrations of indole and several other volatile organic compounds	1978:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	1	106	dep	AIM	165:167	arg1	performance					183:193	The metabolic performance	169:193	AIM The metabolic performance of the gut microbiota	165:215	AIM The metabolic performance of the gut microbiota contributes to the onset of type 2 diabetes.					
37085526	3	107	theme	gut	420:422	arg1	microbiome					424:433	the complex gut microbiome	408:433	the complex gut microbiome	408:433	We hypothesized (1) that the composition of the complex gut microbiome and metabolome (MIME) differ across metabolic spectra (lean-obese-diabetes); (2) that specific MIME patterns could explain the differential responses to dietary inulin; and (3) that the response can be predicted based on baseline MIME signature and clinical characteristics.					
37085526	0	108	theme	new-onset	26:34	arg1	diabetes					36:43	new-onset diabetes	26:43	new-onset diabetes	26:43	Multi-omics signatures in new-onset diabetes predict metabolic response to dietary inulin: findings from an observational study followed by an interventional trial.					
37085526	9	109	dep	Eubacterium	1795:1805	arg1	halii					1807:1811	halii	1807:1811	halii	1807:1811	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	7	110	theme	DM	1231:1232	arg1	groups					1241:1246	the DM and LH groups	1227:1246	the DM and LH groups	1227:1246	While the DM and LH groups represented opposite poles of the abundance spectrum, OB was closer to DM.					
37085526	8	111	theme	microbiome	1465:1474	arg1	composition					1476:1486	microbiome composition	1465:1486	microbiome composition	1465:1486	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37085526	9	112	theme	certain	1756:1762	arg1	Dialister					1858:1866	Dialister	1858:1866	Dialister	1858:1866	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	112	theme	certain	1756:1762	arg1	Blautia					1786:1792	Blautia	1786:1792	Blautia	1786:1792	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	112	theme	certain	1756:1762	arg1	Phascolarctobacterium					1872:1892	Phascolarctobacterium	1872:1892	Phascolarctobacterium	1872:1892	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	112	theme	certain	1756:1762	arg1	Ruminiclostridium					1839:1855	Ruminiclostridium	1839:1855	Ruminiclostridium	1839:1855	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	112	theme	certain	1756:1762	arg1	taxa					1774:1777	certain bacterial taxa	1756:1777	certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium)	1756:1893	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	112	theme	certain	1756:1762	arg1	group					1813:1817	Eubacterium halii group	1795:1817	Eubacterium halii group	1795:1817	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	12	113	theme	nutritional	2390:2400	arg1	interventions					2402:2414	nutritional interventions	2390:2414	nutritional interventions	2390:2414	These could be further validated to personalize nutritional interventions in patients with newly diagnosed diabetes.					
37085526	11	114	from	variability	2209:2219	arg1	effects					2238:2244	the metabolic effects	2224:2244	the metabolic effects of dietary inulin	2224:2262	The large inter-individual variability in the metabolic effects of dietary inulin was explained by differences in baseline glycemic status and MIME signatures.					
37085526	11	115	theme	inter-individual	2192:2207	arg1	variability					2209:2219	The large inter-individual variability	2182:2219	The large inter-individual variability in the metabolic effects of dietary inulin	2182:2262	The large inter-individual variability in the metabolic effects of dietary inulin was explained by differences in baseline glycemic status and MIME signatures.					
37085526	0	116	dep	predict	45:51	arg1	findings					91:98	findings	91:98	findings from an observational study followed by an interventional trial	91:162	Multi-omics signatures in new-onset diabetes predict metabolic response to dietary inulin: findings from an observational study followed by an interventional trial.					
37085526	9	117	theme	taxa	1774:1777	arg1	abundance					1743:1751	the abundance	1739:1751	the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds	1739:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	9	118	theme	other	2021:2025	arg1	compounds					2044:2052	indole and several other volatile organic compounds	2002:2052	compounds	2044:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	2	119	theme	variable	328:335	arg1	response					354:361	the highly variable inter-individual response	317:361	the highly variable inter-individual response	317:361	However, targeted dietary interventions are limited by the highly variable inter-individual response.					
37085526	11	120	theme	large	2186:2190	arg1	variability					2209:2219	The large inter-individual variability	2182:2219	The large inter-individual variability in the metabolic effects of dietary inulin	2182:2262	The large inter-individual variability in the metabolic effects of dietary inulin was explained by differences in baseline glycemic status and MIME signatures.					
37085526	5	121	theme	predictive	1096:1105	arg1	study					1107:1111	a predictive study	1094:1111	a predictive study	1094:1111	Subsequently, 27 DM were recruited for a predictive study: 3 months of dietary inulin (10 g/day) intervention.					
37085526	4	122	theme	rRNA	1013:1016	arg1	sequencing					1018:1027	16 S rRNA sequencing	1008:1027	16 S rRNA sequencing	1008:1027	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	4	122	theme	rRNA	1013:1016	arg1	metabolomes					995:1005	fecal/serum metabolomes	983:1005	fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling)	983:1052	METHOD Forty-nine patients with newly diagnosed pre/diabetes (DM), 66 metabolically healthy overweight/obese (OB), and 32 healthy lean (LH) volunteers were compared in a cross-sectional case-control study integrating clinical variables, dietary intake, gut microbiome, and fecal/serum metabolomes (16 S rRNA sequencing, metabolomics profiling).					
37085526	9	123	theme	organic	2036:2042	arg1	compounds					2044:2052	indole and several other volatile organic compounds	2002:2052	compounds	2044:2052	The improved glycemic outcomes of inulin treatment were dependent on better baseline glycemic status and variables related to the gut microbiota, including the abundance of certain bacterial taxa (i.e., Blautia, Eubacterium halii group, Lachnoclostridium, Ruminiclostridium, Dialister, or Phascolarctobacterium), serum concentrations of branched-chain amino acid derivatives and asparagine, and fecal concentrations of indole and several other volatile organic compounds.					
37085526	8	124	theme	Inulin	1323:1328	arg1	supplementation					1330:1344	Inulin supplementation	1323:1344	Inulin supplementation	1323:1344	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37085526	10	125	theme	glucose	2162:2168	arg1	metabolism					2170:2179	impaired glucose metabolism	2153:2179	impaired glucose metabolism	2153:2179	CONCLUSION We demonstrated that obesity is a stronger determinant of different MIME patterns than impaired glucose metabolism.					
37085526	8	126	theme	increased	1524:1532	arg1	concentrations					1567:1580	increased serum butyric and propionic acid concentrations	1524:1580	increased serum butyric and propionic acid concentrations	1524:1580	Inulin supplementation was associated with an overall improvement in glycemic indices, though the response was very variable, with a shift in microbiome composition toward a more favorable profile and increased serum butyric and propionic acid concentrations.					
37070953	7	0	theme	in	1541:1542	arg1	results					1584:1590	The in vitro wound closure test (scratch test) results	1537:1590	The in vitro wound closure test (scratch test) results	1537:1590	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	4	1	theme	cells	1134:1138	arg1	activity					1108:1115	the metabolic activity	1094:1115	the metabolic activity of breast cancer cells	1094:1138	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	8	2	theme	mineralization	1729:1742	arg1	bioactivity					1714:1724	the bioactivity	1710:1724	the bioactivity of mineralization	1710:1742	Finally, the bioactivity of mineralization was evaluated to demonstrate that these hydrogels can induce the formation of carbonated apatite on their surface.					
37070953	4	3	theme	metabolic	1200:1208	arg1	activity					1210:1217	their metabolic activity	1194:1217	their metabolic activity	1194:1217	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	3	4	theme	alginate	589:596	arg1	content					598:604	alginate content	589:604	alginate content (0-40 wt%)	589:615	The increment in alginate content (0-40 wt%) significantly increases the swelling capacity, generating semi-crystalline granular structures with improved storage modulus and resistance to thermal, hydrolytic, and proteolytic degradation.					
37070953	3	4	theme	alginate	589:596	arg1	%					614:614	0-40 wt%	607:614	0-40 wt%	607:614	The increment in alginate content (0-40 wt%) significantly increases the swelling capacity, generating semi-crystalline granular structures with improved storage modulus and resistance to thermal, hydrolytic, and proteolytic degradation.					
37070953	9	5	theme	tissue	1962:1967	arg1	strategies					1977:1986	tissue healing strategies	1962:1986	tissue healing strategies	1962:1986	The engineered hydrogels show biomedical multifunctionality and they could be applied in soft and hard tissue healing strategies, anticancer therapies, and drug release devices.					
37070953	4	6	theme	cell	1011:1014	arg1	lines					1016:1020	cancer cell lines	1004:1020	cancer cell lines	1004:1020	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	2	7	from	matrices	420:427	arg1	state					445:449	the hydrogel state	432:449	the hydrogel state	432:449	It was determined that the matrices in the hydrogel state are crosslinked by the formation of urea and amide bonds between the biopolymer chains and the polyurethane crosslinker.					
37070953	4	8	theme	colon	1175:1179	arg1	cells					1188:1192	colon cancer cells	1175:1192	colon cancer cells	1175:1192	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	1	9	theme	semi-interpenetrating	212:232	arg1	semi-IPN					254:261	semi-IPN	254:261	semi-IPN	254:261	In this work, hydrogels based on semi-interpenetrating polymeric networks (semi-IPN) based on collagen-polyurethane-alginate were studied physicochemically and from different approaches for biomedical application.					
37070953	1	9	theme	semi-interpenetrating	212:232	arg1	networks					244:251	semi-interpenetrating polymeric networks	212:251	semi-interpenetrating polymeric networks (semi-IPN) based on collagen-polyurethane-alginate	212:302	In this work, hydrogels based on semi-interpenetrating polymeric networks (semi-IPN) based on collagen-polyurethane-alginate were studied physicochemically and from different approaches for biomedical application.					
37070953	7	10	theme	wound	1664:1668	arg1	closure					1670:1676	wound closure	1664:1676	wound closure at 15 days of contact	1664:1698	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	7	11	from	15 days	1681:1687	arg1	improvement					1649:1659	an improvement	1646:1659	an improvement in wound closure at 15 days of contact	1646:1698	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	7	11	from	15 days	1681:1687	arg1	closure					1670:1676	wound closure	1664:1676	wound closure at 15 days of contact	1664:1698	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	9	12	theme	anticancer	1989:1998	arg1	therapies					2000:2008	anticancer therapies	1989:2008	anticancer therapies	1989:2008	The engineered hydrogels show biomedical multifunctionality and they could be applied in soft and hard tissue healing strategies, anticancer therapies, and drug release devices.					
37070953	4	13	theme	cancer	1127:1132	arg1	cells					1134:1138	breast cancer cells	1120:1138	breast cancer cells	1120:1138	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	9	14	dep	soft	1948:1951	arg1	strategies					1977:1986	tissue healing strategies	1962:1986	tissue healing strategies	1962:1986	The engineered hydrogels show biomedical multifunctionality and they could be applied in soft and hard tissue healing strategies, anticancer therapies, and drug release devices.					
37070953	9	14	dep	soft	1948:1951	arg1	therapies					2000:2008	anticancer therapies	1989:2008	anticancer therapies	1989:2008	The engineered hydrogels show biomedical multifunctionality and they could be applied in soft and hard tissue healing strategies, anticancer therapies, and drug release devices.					
37070953	9	14	dep	soft	1948:1951	arg1	devices					2028:2034	drug release devices	2015:2034	drug release devices	2015:2034	The engineered hydrogels show biomedical multifunctionality and they could be applied in soft and hard tissue healing strategies, anticancer therapies, and drug release devices.					
37070953	9	15	theme	drug	2015:2018	arg1	devices					2028:2034	drug release devices	2015:2034	drug release devices	2015:2034	The engineered hydrogels show biomedical multifunctionality and they could be applied in soft and hard tissue healing strategies, anticancer therapies, and drug release devices.					
37070953	3	16	theme	granular	692:699	arg1	structures					701:710	semi-crystalline granular structures	675:710	semi-crystalline granular structures	675:710	The increment in alginate content (0-40 wt%) significantly increases the swelling capacity, generating semi-crystalline granular structures with improved storage modulus and resistance to thermal, hydrolytic, and proteolytic degradation.					
37070953	7	17	with	hydrogel	1610:1617	arg1	alginate					1631:1638	20 wt% alginate	1624:1638	20 wt% alginate	1624:1638	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	7	18	theme	%	1629:1629	arg1	alginate					1631:1638	20 wt% alginate	1624:1638	20 wt% alginate	1624:1638	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	2	19	theme	polyurethane	546:557	arg1	crosslinker					559:569	the polyurethane crosslinker	542:569	the polyurethane crosslinker	542:569	It was determined that the matrices in the hydrogel state are crosslinked by the formation of urea and amide bonds between the biopolymer chains and the polyurethane crosslinker.					
37070953	6	20	theme	inhibition	1434:1443	arg1	higher					1477:1482	higher	1477:1482	higher	1477:1482	The inhibition capacity of Escherichia coli is higher if the polysaccharide concentration is low (10 wt%).					
37070953	6	20	theme	inhibition	1434:1443	arg1	capacity					1445:1452	The inhibition capacity	1430:1452	The inhibition capacity of Escherichia coli	1430:1472	The inhibition capacity of Escherichia coli is higher if the polysaccharide concentration is low (10 wt%).					
37070953	0	21	theme	collagen-polyurethane-alginate	69:98	arg1	networks					57:64	semi-interpenetrating polymeric networks	25:64	semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies	25:176	Smart hydrogels based on semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies.					
37070953	8	22	theme	apatite	1833:1839	arg1	formation					1809:1817	the formation	1805:1817	the formation of carbonated apatite on their surface	1805:1856	Finally, the bioactivity of mineralization was evaluated to demonstrate that these hydrogels can induce the formation of carbonated apatite on their surface.					
37070953	4	23	theme	contact	1243:1249	arg1	72 h					1235:1238	72 h	1235:1238	72 h of contact for the hydrogel with 40 wt% alginate	1235:1287	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	5	24	theme	multidose	1322:1330	arg1	release					1332:1338	multidose release	1322:1338	multidose release of ketorolac	1322:1351	The matrices show a behavior of multidose release of ketorolac, and a higher concentration of analgesic is released in the semi-IPN matrix.					
37070953	0	25	theme	soft/hard	104:112	arg1	healing					121:127	soft/hard tissue healing	104:127	soft/hard tissue healing	104:127	Smart hydrogels based on semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies.					
37070953	7	26	from	improvement	1649:1659	arg1	15 days					1681:1687	15 days	1681:1687	15 days of contact	1681:1698	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	7	26	from	improvement	1649:1659	arg1	closure					1670:1676	wound closure	1664:1676	wound closure at 15 days of contact	1664:1698	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	0	27	theme	drug	130:133	arg1	devices					144:150	drug delivery devices	130:150	drug delivery devices	130:150	Smart hydrogels based on semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies.					
37070953	2	28	theme	amide	496:500	arg1	bonds					502:506	urea and amide bonds	487:506	bonds	502:506	It was determined that the matrices in the hydrogel state are crosslinked by the formation of urea and amide bonds between the biopolymer chains and the polyurethane crosslinker.					
37070953	1	29	theme	different	344:352	arg1	approaches					354:363	different approaches	344:363	different approaches for biomedical application	344:390	In this work, hydrogels based on semi-interpenetrating polymeric networks (semi-IPN) based on collagen-polyurethane-alginate were studied physicochemically and from different approaches for biomedical application.					
37070953	4	30	theme	%	1278:1278	arg1	alginate					1280:1287	40 wt% alginate	1273:1287	40 wt% alginate	1273:1287	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	4	31	theme	in	814:815	arg1	results					835:841	The in vitro bioactivity results	810:841	The in vitro bioactivity results	810:841	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	2	32	theme	hydrogel	436:443	arg1	state					445:449	the hydrogel state	432:449	the hydrogel state	432:449	It was determined that the matrices in the hydrogel state are crosslinked by the formation of urea and amide bonds between the biopolymer chains and the polyurethane crosslinker.					
37070953	7	33	theme	contact	1692:1698	arg1	15 days					1681:1687	15 days	1681:1687	15 days of contact	1681:1698	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	2	34	theme	urea	487:490	arg1	bonds					502:506	urea and amide bonds	487:506	bonds	502:506	It was determined that the matrices in the hydrogel state are crosslinked by the formation of urea and amide bonds between the biopolymer chains and the polyurethane crosslinker.					
37070953	4	35	theme	bioactivity	823:833	arg1	results					835:841	The in vitro bioactivity results	810:841	The in vitro bioactivity results	810:841	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	7	36	theme	test	1578:1581	arg1	results					1584:1590	The in vitro wound closure test (scratch test) results	1537:1590	The in vitro wound closure test (scratch test) results	1537:1590	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	2	37	theme	biopolymer	520:529	arg1	chains					531:536	the biopolymer chains	516:536	the biopolymer chains	516:536	It was determined that the matrices in the hydrogel state are crosslinked by the formation of urea and amide bonds between the biopolymer chains and the polyurethane crosslinker.					
37070953	3	38	theme	storage	726:732	arg1	modulus					734:740	improved storage modulus	717:740	improved storage modulus	717:740	The increment in alginate content (0-40 wt%) significantly increases the swelling capacity, generating semi-crystalline granular structures with improved storage modulus and resistance to thermal, hydrolytic, and proteolytic degradation.					
37070953	5	39	theme	analgesic	1384:1392	arg1	concentration					1367:1379	a higher concentration	1358:1379	a higher concentration of analgesic	1358:1392	The matrices show a behavior of multidose release of ketorolac, and a higher concentration of analgesic is released in the semi-IPN matrix.					
37070953	0	40	theme	semi-interpenetrating	25:45	arg1	networks					57:64	semi-interpenetrating polymeric networks	25:64	semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies	25:176	Smart hydrogels based on semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies.					
37070953	4	41	theme	biomaterials	1071:1082	arg1	composition					1050:1060	the composition	1046:1060	the composition of these biomaterials	1046:1082	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	4	42	theme	metabolic	1098:1106	arg1	activity					1108:1115	the metabolic activity	1094:1115	the metabolic activity of breast cancer cells	1094:1138	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	4	43	theme	metabolic	914:922	arg1	activity					924:931	the metabolic activity	910:931	the metabolic activity of monocytes and fibroblasts	910:960	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	6	44	theme	coli	1469:1472	arg1	higher					1477:1482	higher	1477:1482	higher	1477:1482	The inhibition capacity of Escherichia coli is higher if the polysaccharide concentration is low (10 wt%).					
37070953	6	44	theme	coli	1469:1472	arg1	capacity					1445:1452	The inhibition capacity	1430:1452	The inhibition capacity of Escherichia coli	1430:1472	The inhibition capacity of Escherichia coli is higher if the polysaccharide concentration is low (10 wt%).					
37070953	1	45	theme	biomedical	369:378	arg1	application					380:390	biomedical application	369:390	biomedical application	369:390	In this work, hydrogels based on semi-interpenetrating polymeric networks (semi-IPN) based on collagen-polyurethane-alginate were studied physicochemically and from different approaches for biomedical application.					
37070953	7	46	theme	closure	1556:1562	arg1	results					1584:1590	The in vitro wound closure test (scratch test) results	1537:1590	The in vitro wound closure test (scratch test) results	1537:1590	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	9	47	theme	biomedical	1889:1898	arg1	multifunctionality					1900:1917	biomedical multifunctionality	1889:1917	biomedical multifunctionality	1889:1917	The engineered hydrogels show biomedical multifunctionality and they could be applied in soft and hard tissue healing strategies, anticancer therapies, and drug release devices.					
37070953	4	48	theme	novel	883:887	arg1	hydrogels					889:897	these novel hydrogels	877:897	these novel hydrogels	877:897	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	4	49	theme	fibroblasts	950:960	arg1	activity					924:931	the metabolic activity	910:931	the metabolic activity of monocytes and fibroblasts	910:960	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	4	50	with	contact	1243:1249	arg1	alginate					1280:1287	40 wt% alginate	1273:1287	40 wt% alginate	1273:1287	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	3	51	from	increment	576:584	arg1	content					598:604	alginate content	589:604	alginate content (0-40 wt%)	589:615	The increment in alginate content (0-40 wt%) significantly increases the swelling capacity, generating semi-crystalline granular structures with improved storage modulus and resistance to thermal, hydrolytic, and proteolytic degradation.					
37070953	3	51	from	increment	576:584	arg1	%					614:614	0-40 wt%	607:614	0-40 wt%	607:614	The increment in alginate content (0-40 wt%) significantly increases the swelling capacity, generating semi-crystalline granular structures with improved storage modulus and resistance to thermal, hydrolytic, and proteolytic degradation.					
37070953	3	52	theme	proteolytic	785:795	arg1	degradation					797:807	proteolytic degradation	785:807	proteolytic degradation	785:807	The increment in alginate content (0-40 wt%) significantly increases the swelling capacity, generating semi-crystalline granular structures with improved storage modulus and resistance to thermal, hydrolytic, and proteolytic degradation.					
37070953	4	53	theme	monocytes	936:944	arg1	activity					924:931	the metabolic activity	910:931	the metabolic activity of monocytes and fibroblasts	910:960	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	9	54	theme	healing	1969:1975	arg1	strategies					1977:1986	tissue healing strategies	1962:1986	tissue healing strategies	1962:1986	The engineered hydrogels show biomedical multifunctionality and they could be applied in soft and hard tissue healing strategies, anticancer therapies, and drug release devices.					
37070953	4	55	theme	cancer	1004:1009	arg1	lines					1016:1020	cancer cell lines	1004:1020	cancer cell lines	1004:1020	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	4	56	theme	cancer	1181:1186	arg1	cells					1188:1192	colon cancer cells	1175:1192	colon cancer cells	1175:1192	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	0	57	theme	anticancer	157:166	arg1	therapies					168:176	anticancer therapies	157:176	anticancer therapies	157:176	Smart hydrogels based on semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies.					
37070953	3	58	theme	swelling	645:652	arg1	capacity					654:661	the swelling capacity	641:661	the swelling capacity	641:661	The increment in alginate content (0-40 wt%) significantly increases the swelling capacity, generating semi-crystalline granular structures with improved storage modulus and resistance to thermal, hydrolytic, and proteolytic degradation.					
37070953	1	59	theme	polymeric	234:242	arg1	semi-IPN					254:261	semi-IPN	254:261	semi-IPN	254:261	In this work, hydrogels based on semi-interpenetrating polymeric networks (semi-IPN) based on collagen-polyurethane-alginate were studied physicochemically and from different approaches for biomedical application.					
37070953	1	59	theme	polymeric	234:242	arg1	networks					244:251	semi-interpenetrating polymeric networks	212:251	semi-interpenetrating polymeric networks (semi-IPN) based on collagen-polyurethane-alginate	212:302	In this work, hydrogels based on semi-interpenetrating polymeric networks (semi-IPN) based on collagen-polyurethane-alginate were studied physicochemically and from different approaches for biomedical application.					
37070953	8	60	from	formation	1809:1817	arg1	surface					1850:1856	their surface	1844:1856	their surface	1844:1856	Finally, the bioactivity of mineralization was evaluated to demonstrate that these hydrogels can induce the formation of carbonated apatite on their surface.					
37070953	4	61	theme	40 wt	1273:1277	arg1	alginate					1280:1287	40 wt% alginate	1273:1287	40 wt% alginate	1273:1287	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	5	62	theme	ketorolac	1343:1351	arg1	release					1332:1338	multidose release	1322:1338	multidose release of ketorolac	1322:1351	The matrices show a behavior of multidose release of ketorolac, and a higher concentration of analgesic is released in the semi-IPN matrix.					
37070953	3	63	theme	semi-crystalline	675:690	arg1	structures					701:710	semi-crystalline granular structures	675:710	semi-crystalline granular structures	675:710	The increment in alginate content (0-40 wt%) significantly increases the swelling capacity, generating semi-crystalline granular structures with improved storage modulus and resistance to thermal, hydrolytic, and proteolytic degradation.					
37070953	4	64	dep	in	814:815	arg1	vitro					817:821	vitro	817:821	vitro	817:821	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	7	65	theme	20 wt	1624:1628	arg1	alginate					1631:1638	20 wt% alginate	1624:1638	20 wt% alginate	1624:1638	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	9	66	theme	release	2020:2026	arg1	devices					2028:2034	drug release devices	2015:2034	drug release devices	2015:2034	The engineered hydrogels show biomedical multifunctionality and they could be applied in soft and hard tissue healing strategies, anticancer therapies, and drug release devices.					
37070953	0	67	theme	tissue	114:119	arg1	healing					121:127	soft/hard tissue healing	104:127	soft/hard tissue healing	104:127	Smart hydrogels based on semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies.					
37070953	8	68	theme	carbonated	1822:1831	arg1	apatite					1833:1839	carbonated apatite	1822:1839	carbonated apatite	1822:1839	Finally, the bioactivity of mineralization was evaluated to demonstrate that these hydrogels can induce the formation of carbonated apatite on their surface.					
37070953	5	69	theme	release	1332:1338	arg1	behavior					1310:1317	a behavior	1308:1317	a behavior of multidose release of ketorolac	1308:1351	The matrices show a behavior of multidose release of ketorolac, and a higher concentration of analgesic is released in the semi-IPN matrix.					
37070953	0	70	theme	delivery	135:142	arg1	devices					144:150	drug delivery devices	130:150	drug delivery devices	130:150	Smart hydrogels based on semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies.					
37070953	5	71	theme	higher	1360:1365	arg1	concentration					1367:1379	a higher concentration	1358:1379	a higher concentration of analgesic	1358:1392	The matrices show a behavior of multidose release of ketorolac, and a higher concentration of analgesic is released in the semi-IPN matrix.					
37070953	6	72	theme	polysaccharide	1491:1504	arg1	low					1523:1525	low	1523:1525	low	1523:1525	The inhibition capacity of Escherichia coli is higher if the polysaccharide concentration is low (10 wt%).					
37070953	6	72	theme	polysaccharide	1491:1504	arg1	%					1533:1533	10 wt%	1528:1533	10 wt%	1528:1533	The inhibition capacity of Escherichia coli is higher if the polysaccharide concentration is low (10 wt%).					
37070953	6	72	theme	polysaccharide	1491:1504	arg1	concentration					1506:1518	the polysaccharide concentration	1487:1518	the polysaccharide concentration	1487:1518	The inhibition capacity of Escherichia coli is higher if the polysaccharide concentration is low (10 wt%).					
37070953	4	73	theme	breast	1120:1125	arg1	cells					1134:1138	breast cancer cells	1120:1138	breast cancer cells	1120:1138	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	3	74	theme	improved	717:724	arg1	modulus					734:740	improved storage modulus	717:740	improved storage modulus	717:740	The increment in alginate content (0-40 wt%) significantly increases the swelling capacity, generating semi-crystalline granular structures with improved storage modulus and resistance to thermal, hydrolytic, and proteolytic degradation.					
37070953	0	75	theme	polymeric	47:55	arg1	networks					57:64	semi-interpenetrating polymeric networks	25:64	semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies	25:176	Smart hydrogels based on semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies.					
37070953	7	76	dep	in	1541:1542	arg1	vitro					1544:1548	vitro	1544:1548	vitro	1544:1548	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	9	77	theme	engineered	1863:1872	arg1	hydrogels					1874:1882	The engineered hydrogels	1859:1882	The engineered hydrogels	1859:1882	The engineered hydrogels show biomedical multifunctionality and they could be applied in soft and hard tissue healing strategies, anticancer therapies, and drug release devices.					
37070953	4	78	theme	hydrogels	889:897	arg1	composition					862:872	the composition	858:872	the composition of these novel hydrogels	858:897	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	7	79	theme	scratch	1570:1576	arg1	results					1584:1590	The in vitro wound closure test (scratch test) results	1537:1590	The in vitro wound closure test (scratch test) results	1537:1590	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	6	80	dep	low	1523:1525	arg1	low					1523:1525	low	1523:1525	low	1523:1525	The inhibition capacity of Escherichia coli is higher if the polysaccharide concentration is low (10 wt%).					
37070953	6	80	dep	low	1523:1525	arg1	%					1533:1533	10 wt%	1528:1533	10 wt%	1528:1533	The inhibition capacity of Escherichia coli is higher if the polysaccharide concentration is low (10 wt%).					
37070953	6	80	dep	low	1523:1525	arg1	concentration					1506:1518	the polysaccharide concentration	1487:1518	the polysaccharide concentration	1487:1518	The inhibition capacity of Escherichia coli is higher if the polysaccharide concentration is low (10 wt%).					
37070953	2	81	theme	bonds	502:506	arg1	formation					474:482	the formation	470:482	the formation of urea and amide bonds between the biopolymer chains and the polyurethane crosslinker	470:569	It was determined that the matrices in the hydrogel state are crosslinked by the formation of urea and amide bonds between the biopolymer chains and the polyurethane crosslinker.					
37070953	7	82	theme	test	1564:1567	arg1	results					1584:1590	The in vitro wound closure test (scratch test) results	1537:1590	The in vitro wound closure test (scratch test) results	1537:1590	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	4	83	theme	stimulation	1154:1164	arg1	48 h					1146:1149	48 h	1146:1149	48 h of stimulation	1146:1164	The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate.					
37070953	5	84	theme	semi-IPN	1413:1420	arg1	matrix					1422:1427	the semi-IPN matrix	1409:1427	the semi-IPN matrix	1409:1427	The matrices show a behavior of multidose release of ketorolac, and a higher concentration of analgesic is released in the semi-IPN matrix.					
37070953	7	85	theme	wound	1550:1554	arg1	results					1584:1590	The in vitro wound closure test (scratch test) results	1537:1590	The in vitro wound closure test (scratch test) results	1537:1590	The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact.					
37070953	0	86	dep	hydrogels	6:14	arg1	based					16:20	based	16:20	hydrogels based on semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies	6:176	Smart hydrogels based on semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies.					
36273545	11	0	contain	have	1401:1404	arg1	pulps					1395:1399	Unbleached cellulose pulps	1374:1399	Unbleached cellulose pulps	1374:1399	Unbleached cellulose pulps have excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery.					
36273545	11	0	contain	have	1401:1404	arg2	potential					1416:1424	excellent potential	1406:1424	excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery	1406:1518	Unbleached cellulose pulps have excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery.					
36273545	8	1	theme	dominant	967:974	arg1	role					976:979	a dominant role	965:979	a dominant role	965:979	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	4	2	theme	compositions	595:606	arg1	effect					570:575	the effect	566:575	the effect of alkali solvent compositions on hydrogel formation	566:628	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	0	3	from	pulps	64:68	arg1	development					19:29	Cellulose hydrogel development	0:29	Cellulose hydrogel development from unbleached oil palm biomass pulps for dermal drug delivery.	0:94	Cellulose hydrogel development from unbleached oil palm biomass pulps for dermal drug delivery.					
36273545	7	4	with	acceptable	896:905	arg1	cytotoxicity					934:945	low cytotoxicity	930:945	low cytotoxicity	930:945	Light brownish hydrogels formed are aesthetically acceptable and biodegradable with low cytotoxicity.					
36273545	4	5	theme	hydrogel	611:618	arg1	formation					620:628	hydrogel formation	611:628	hydrogel formation	611:628	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	5	6	theme	sodium	706:711	arg1	NaOH					724:727	NaOH	724:727	NaOH	724:727	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	5	6	theme	sodium	706:711	arg1	hydroxide					713:721	sodium hydroxide	706:721	sodium hydroxide (NaOH) (6-8%w/v)	706:738	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	5	6	theme	sodium	706:711	arg1	w/v					735:737	6-8%w/v	731:737	6-8%w/v	731:737	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	11	7	theme	drug	1506:1509	arg1	delivery					1511:1518	dermal drug delivery	1499:1518	dermal drug delivery	1499:1518	Unbleached cellulose pulps have excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery.					
36273545	7	8	theme	brownish	852:859	arg1	hydrogels					861:869	Light brownish hydrogels	846:869	Light brownish hydrogels formed	846:876	Light brownish hydrogels formed are aesthetically acceptable and biodegradable with low cytotoxicity.					
36273545	10	9	theme	hydrogels	1363:1371	arg1	ratio					1354:1358	the swelling ratio	1341:1358	the swelling ratio of hydrogels	1341:1371	Most hydrogels showed >80 % of ibuprofen permeated into the skin and this increased with the swelling ratio of hydrogels.					
36273545	3	10	theme	environmental	294:306	arg1	impacts					308:314	the detrimental environmental impacts	278:314	the detrimental environmental impacts of bleaching reagents	278:336	Considering the detrimental environmental impacts of bleaching reagents, unbleached non-woody biomass was proposed as an alternative.					
36273545	8	11	theme	crosslinked	1020:1030	arg1	walls					1040:1044	thinner and deformable crosslinked network walls	997:1044	thinner and deformable crosslinked network walls in a porous hydrogel structure	997:1075	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	8	12	contain	has	961:963	arg2	role					976:979	a dominant role	965:979	a dominant role	965:979	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	8	12	contain	has	961:963	arg1	content					953:959	NaOH content	948:959	NaOH content	948:959	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	9	13	theme	%	1208:1208	arg1	NaOH					1213:1216	7%w/v NaOH	1207:1216	7%w/v NaOH	1207:1216	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	8	14	from	walls	1040:1044	arg1	structure					1067:1075	a porous hydrogel structure	1049:1075	a porous hydrogel structure	1049:1075	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	2	15	theme	available	230:238	arg1	cellulose					255:263	readily available bleached woody cellulose	222:263	readily available bleached woody cellulose	222:263	Current cellulose hydrogel developments commonly focus on readily available bleached woody cellulose.					
36273545	3	16	theme	reagents	329:336	arg1	impacts					308:314	the detrimental environmental impacts	278:314	the detrimental environmental impacts of bleaching reagents	278:336	Considering the detrimental environmental impacts of bleaching reagents, unbleached non-woody biomass was proposed as an alternative.					
36273545	4	17	theme	oil	481:483	arg1	bunches					502:508	oil palm empty fruit bunches	481:508	oil palm empty fruit bunches for dermal drug delivery	481:533	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	9	18	theme	4	1222:1222	arg1	%					1223:1223	%	1223:1223	%	1223:1223	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	1	19	theme	attractive	113:122	arg1	platform					124:131	an attractive platform	110:131	an attractive platform for drug delivery to the skin	110:161	Hydrogels are an attractive platform for drug delivery to the skin.					
36273545	1	19	theme	attractive	113:122	arg1	Hydrogels					96:104	Hydrogels	96:104	Hydrogels	96:104	Hydrogels are an attractive platform for drug delivery to the skin.					
36273545	11	20	theme	excellent	1456:1464	arg1	properties					1484:1493	excellent physicomechanical properties	1456:1493	excellent physicomechanical properties for dermal drug delivery	1456:1518	Unbleached cellulose pulps have excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery.					
36273545	9	21	theme	w/v	1224:1226	arg1	urea					1228:1231	4%w/v urea	1222:1231	4%w/v urea	1222:1231	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	4	22	theme	native	449:454	arg1	cellulose					456:464	native cellulose	449:464	native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation	449:628	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	5	23	theme	%	753:753	arg1	urea					744:747	urea	744:747	urea (4-6%w/v)	744:757	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	5	23	theme	%	753:753	arg1	w/v					754:756	4-6%w/v	750:756	4-6%w/v	750:756	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	2	24	theme	woody	249:253	arg1	cellulose					255:263	readily available bleached woody cellulose	222:263	readily available bleached woody cellulose	222:263	Current cellulose hydrogel developments commonly focus on readily available bleached woody cellulose.					
36273545	8	25	theme	NaOH	1102:1105	arg1	content					1107:1113	high NaOH content	1097:1113	high NaOH content	1097:1113	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	3	26	theme	unbleached	339:348	arg1	biomass					360:366	unbleached non-woody biomass	339:366	unbleached non-woody biomass	339:366	Considering the detrimental environmental impacts of bleaching reagents, unbleached non-woody biomass was proposed as an alternative.					
36273545	3	26	theme	unbleached	339:348	arg1	alternative					387:397	an alternative	384:397	an alternative	384:397	Considering the detrimental environmental impacts of bleaching reagents, unbleached non-woody biomass was proposed as an alternative.					
36273545	4	27	theme	dermal	514:519	arg1	delivery					526:533	dermal drug delivery	514:533	dermal drug delivery	514:533	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	11	28	theme	dermal	1499:1504	arg1	delivery					1511:1518	dermal drug delivery	1499:1518	dermal drug delivery	1499:1518	Unbleached cellulose pulps have excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery.					
36273545	9	29	located	observed	1195:1202	arg1	NaOH					1213:1216	7%w/v NaOH	1207:1216	7%w/v NaOH	1207:1216	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	9	29	located	observed	1195:1202	arg2	effects					1128:1134	Synergistic effects	1116:1134	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %)	1116:1188	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	9	29	located	observed	1195:1202	arg1	urea					1228:1231	4%w/v urea	1222:1231	4%w/v urea	1222:1231	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	0	30	theme	dermal	74:79	arg1	delivery					86:93	dermal drug delivery	74:93	dermal drug delivery	74:93	Cellulose hydrogel development from unbleached oil palm biomass pulps for dermal drug delivery.					
36273545	5	31	theme	Unbleached	631:640	arg1	pulps					653:657	Unbleached dissolving pulps	631:657	Unbleached dissolving pulps	631:657	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	4	32	theme	empty	490:494	arg1	bunches					502:508	oil palm empty fruit bunches	481:508	oil palm empty fruit bunches for dermal drug delivery	481:533	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	8	33	theme	hydrogel	1058:1065	arg1	structure					1067:1075	a porous hydrogel structure	1049:1075	a porous hydrogel structure	1049:1075	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	11	34	theme	Unbleached	1374:1383	arg1	pulps					1395:1399	Unbleached cellulose pulps	1374:1399	Unbleached cellulose pulps	1374:1399	Unbleached cellulose pulps have excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery.					
36273545	0	35	theme	Cellulose	0:8	arg1	development					19:29	Cellulose hydrogel development	0:29	Cellulose hydrogel development from unbleached oil palm biomass pulps for dermal drug delivery.	0:94	Cellulose hydrogel development from unbleached oil palm biomass pulps for dermal drug delivery.					
36273545	5	36	from	solubilised	664:674	arg1	solvents					686:693	alkali solvents	679:693	alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking	679:777	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	9	37	theme	Synergistic	1116:1126	arg1	effects					1128:1134	Synergistic effects	1116:1134	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %)	1116:1188	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	11	38	theme	excellent	1406:1414	arg1	potential					1416:1424	excellent potential	1406:1424	excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery	1406:1518	Unbleached cellulose pulps have excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery.					
36273545	9	39	dep	%	1162:1162	arg1	%					1187:1187	~2800 %	1181:1187	cellulose solubility: 94 %; swelling ratio: ~2800 %	1137:1187	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	9	39	dep	%	1162:1162	arg1	ratio					1174:1178	swelling ratio	1165:1178	cellulose solubility: 94 %; swelling ratio: ~2800 %	1137:1187	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	2	40	theme	Current	164:170	arg1	developments					191:202	Current cellulose hydrogel developments	164:202	Current cellulose hydrogel developments	164:202	Current cellulose hydrogel developments commonly focus on readily available bleached woody cellulose.					
36273545	10	41	theme	Most	1252:1255	arg1	hydrogels					1257:1265	Most hydrogels	1252:1265	Most hydrogels	1252:1265	Most hydrogels showed >80 % of ibuprofen permeated into the skin and this increased with the swelling ratio of hydrogels.					
36273545	8	42	link	crosslinked	1020:1030	arg1	walls					1040:1044	thinner and deformable crosslinked network walls	997:1044	thinner and deformable crosslinked network walls in a porous hydrogel structure	997:1075	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	0	43	theme	unbleached	36:45	arg1	pulps					64:68	unbleached oil palm biomass pulps	36:68	unbleached oil palm biomass pulps for dermal drug delivery	36:93	Cellulose hydrogel development from unbleached oil palm biomass pulps for dermal drug delivery.					
36273545	10	44	theme	ibuprofen	1283:1291	arg1	ibuprofen					1283:1291	ibuprofen	1283:1291	ibuprofen	1283:1291	Most hydrogels showed >80 % of ibuprofen permeated into the skin and this increased with the swelling ratio of hydrogels.					
36273545	10	44	theme	ibuprofen	1283:1291	arg1	%					1278:1278	>80 %	1274:1278	>80 % of ibuprofen	1274:1291	Most hydrogels showed >80 % of ibuprofen permeated into the skin and this increased with the swelling ratio of hydrogels.					
36273545	0	45	theme	palm	51:54	arg1	pulps					64:68	unbleached oil palm biomass pulps	36:68	unbleached oil palm biomass pulps for dermal drug delivery	36:93	Cellulose hydrogel development from unbleached oil palm biomass pulps for dermal drug delivery.					
36273545	7	46	theme	low	930:932	arg1	cytotoxicity					934:945	low cytotoxicity	930:945	low cytotoxicity	930:945	Light brownish hydrogels formed are aesthetically acceptable and biodegradable with low cytotoxicity.					
36273545	6	47	theme	skin	821:824	arg1	studies					837:843	skin permeation studies	821:843	skin permeation studies	821:843	Hydrogels were loaded with ibuprofen for skin permeation studies.					
36273545	9	48	dep	effects	1128:1134	arg1	%					1162:1162	cellulose solubility: 94 %	1137:1162	cellulose solubility: 94 %; swelling ratio: ~2800 %	1137:1187	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	5	49	contain	containing	695:704	arg2	hydroxide					713:721	sodium hydroxide	706:721	sodium hydroxide (NaOH) (6-8%w/v)	706:738	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	5	49	contain	containing	695:704	arg2	NaOH					724:727	NaOH	724:727	NaOH	724:727	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	5	49	contain	containing	695:704	arg2	urea					744:747	urea	744:747	urea (4-6%w/v)	744:757	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	5	49	contain	containing	695:704	arg2	w/v					735:737	6-8%w/v	731:737	6-8%w/v	731:737	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	5	49	contain	containing	695:704	arg1	solvents					686:693	alkali solvents	679:693	alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking	679:777	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	5	49	contain	containing	695:704	arg2	w/v					754:756	4-6%w/v	750:756	4-6%w/v	750:756	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	2	50	theme	hydrogel	182:189	arg1	developments					191:202	Current cellulose hydrogel developments	164:202	Current cellulose hydrogel developments	164:202	Current cellulose hydrogel developments commonly focus on readily available bleached woody cellulose.					
36273545	5	51	theme	alkali	679:684	arg1	solvents					686:693	alkali solvents	679:693	alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking	679:777	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	4	52	theme	solvent	587:593	arg1	compositions					595:606	alkali solvent compositions	580:606	alkali solvent compositions	580:606	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	9	53	theme	solubility	1147:1156	arg1	%					1162:1162	cellulose solubility: 94 %	1137:1162	cellulose solubility: 94 %; swelling ratio: ~2800 %	1137:1187	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	8	54	theme	thinner	997:1003	arg1	walls					1040:1044	thinner and deformable crosslinked network walls	997:1044	thinner and deformable crosslinked network walls in a porous hydrogel structure	997:1075	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	8	55	theme	deformable	1009:1018	arg1	walls					1040:1044	thinner and deformable crosslinked network walls	997:1044	thinner and deformable crosslinked network walls in a porous hydrogel structure	997:1075	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	7	56	theme	Light	846:850	arg1	hydrogels					861:869	Light brownish hydrogels	846:869	Light brownish hydrogels formed	846:876	Light brownish hydrogels formed are aesthetically acceptable and biodegradable with low cytotoxicity.					
36273545	8	57	theme	network	1032:1038	arg1	walls					1040:1044	thinner and deformable crosslinked network walls	997:1044	thinner and deformable crosslinked network walls in a porous hydrogel structure	997:1075	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	10	58	theme	swelling	1345:1352	arg1	ratio					1354:1358	the swelling ratio	1341:1358	the swelling ratio of hydrogels	1341:1371	Most hydrogels showed >80 % of ibuprofen permeated into the skin and this increased with the swelling ratio of hydrogels.					
36273545	5	59	theme	%	734:734	arg1	hydroxide					713:721	sodium hydroxide	706:721	sodium hydroxide (NaOH) (6-8%w/v)	706:738	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	5	59	theme	%	734:734	arg1	w/v					735:737	6-8%w/v	731:737	6-8%w/v	731:737	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	9	60	theme	w/v	1209:1211	arg1	NaOH					1213:1216	7%w/v NaOH	1207:1216	7%w/v NaOH	1207:1216	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	3	61	theme	detrimental	282:292	arg1	impacts					308:314	the detrimental environmental impacts	278:314	the detrimental environmental impacts of bleaching reagents	278:336	Considering the detrimental environmental impacts of bleaching reagents, unbleached non-woody biomass was proposed as an alternative.					
36273545	4	62	theme	palm	485:488	arg1	bunches					502:508	oil palm empty fruit bunches	481:508	oil palm empty fruit bunches for dermal drug delivery	481:533	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	2	63	dep	available	230:238	arg1	bleached					240:247	bleached	240:247	bleached	240:247	Current cellulose hydrogel developments commonly focus on readily available bleached woody cellulose.					
36273545	4	64	from	cellulose	456:464	arg1	hydrogel					435:442	hydrogel	435:442	hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation	435:628	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	9	65	theme	7	1207:1207	arg1	%					1208:1208	%	1208:1208	%	1208:1208	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	9	66	theme	%	1223:1223	arg1	urea					1228:1231	4%w/v urea	1222:1231	4%w/v urea	1222:1231	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	10	67	dep	showed	1267:1272	arg1	permeated					1293:1301	permeated	1293:1301	showed >80 % of ibuprofen permeated into the skin	1267:1315	Most hydrogels showed >80 % of ibuprofen permeated into the skin and this increased with the swelling ratio of hydrogels.					
36273545	3	68	theme	bleaching	319:327	arg1	reagents					329:336	bleaching reagents	319:336	bleaching reagents	319:336	Considering the detrimental environmental impacts of bleaching reagents, unbleached non-woody biomass was proposed as an alternative.					
36273545	11	69	theme	hydrogel	1430:1437	arg1	fabrication					1439:1449	hydrogel fabrication	1430:1449	hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery	1430:1518	Unbleached cellulose pulps have excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery.					
36273545	1	70	theme	drug	137:140	arg1	delivery					142:149	drug delivery	137:149	drug delivery to the skin	137:161	Hydrogels are an attractive platform for drug delivery to the skin.					
36273545	4	71	theme	drug	521:524	arg1	delivery					526:533	dermal drug delivery	514:533	dermal drug delivery	514:533	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	0	72	theme	drug	81:84	arg1	delivery					86:93	dermal drug delivery	74:93	dermal drug delivery	74:93	Cellulose hydrogel development from unbleached oil palm biomass pulps for dermal drug delivery.					
36273545	9	73	theme	low	1238:1240	arg1	toxicity					1242:1249	low toxicity	1238:1249	low toxicity	1238:1249	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	3	74	theme	non-woody	350:358	arg1	biomass					360:366	unbleached non-woody biomass	339:366	unbleached non-woody biomass	339:366	Considering the detrimental environmental impacts of bleaching reagents, unbleached non-woody biomass was proposed as an alternative.					
36273545	3	74	theme	non-woody	350:358	arg1	alternative					387:397	an alternative	384:397	an alternative	384:397	Considering the detrimental environmental impacts of bleaching reagents, unbleached non-woody biomass was proposed as an alternative.					
36273545	11	75	theme	physicomechanical	1466:1482	arg1	properties					1484:1493	excellent physicomechanical properties	1456:1493	excellent physicomechanical properties for dermal drug delivery	1456:1518	Unbleached cellulose pulps have excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery.					
36273545	8	76	theme	porous	1051:1056	arg1	structure					1067:1075	a porous hydrogel structure	1049:1075	a porous hydrogel structure	1049:1075	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	4	77	theme	fruit	496:500	arg1	bunches					502:508	oil palm empty fruit bunches	481:508	oil palm empty fruit bunches for dermal drug delivery	481:533	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	11	78	theme	cellulose	1385:1393	arg1	pulps					1395:1399	Unbleached cellulose pulps	1374:1399	Unbleached cellulose pulps	1374:1399	Unbleached cellulose pulps have excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery.					
36273545	5	79	theme	dissolving	642:651	arg1	pulps					653:657	Unbleached dissolving pulps	631:657	Unbleached dissolving pulps	631:657	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	0	80	theme	hydrogel	10:17	arg1	development					19:29	Cellulose hydrogel development	0:29	Cellulose hydrogel development from unbleached oil palm biomass pulps for dermal drug delivery.	0:94	Cellulose hydrogel development from unbleached oil palm biomass pulps for dermal drug delivery.					
36273545	11	81	with	fabrication	1439:1449	arg1	properties					1484:1493	excellent physicomechanical properties	1456:1493	excellent physicomechanical properties for dermal drug delivery	1456:1518	Unbleached cellulose pulps have excellent potential for hydrogel fabrication with excellent physicomechanical properties for dermal drug delivery.					
36273545	4	82	from	effect	570:575	arg1	formation					620:628	hydrogel formation	611:628	hydrogel formation	611:628	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	8	83	theme	high	1097:1100	arg1	content					1107:1113	high NaOH content	1097:1113	high NaOH content	1097:1113	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	4	84	theme	alkali	580:585	arg1	compositions					595:606	alkali solvent compositions	580:606	alkali solvent compositions	580:606	Herein, this study aims to develop hydrogel from native cellulose extracted from oil palm empty fruit bunches for dermal drug delivery with an emphasis on evaluating the effect of alkali solvent compositions on hydrogel formation.					
36273545	2	85	theme	cellulose	172:180	arg1	developments					191:202	Current cellulose hydrogel developments	164:202	Current cellulose hydrogel developments	164:202	Current cellulose hydrogel developments commonly focus on readily available bleached woody cellulose.					
36273545	0	86	theme	oil	47:49	arg1	pulps					64:68	unbleached oil palm biomass pulps	36:68	unbleached oil palm biomass pulps for dermal drug delivery	36:93	Cellulose hydrogel development from unbleached oil palm biomass pulps for dermal drug delivery.					
36273545	9	87	theme	cellulose	1137:1145	arg1	%					1162:1162	cellulose solubility: 94 %	1137:1162	cellulose solubility: 94 %; swelling ratio: ~2800 %	1137:1187	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36273545	0	88	theme	biomass	56:62	arg1	pulps					64:68	unbleached oil palm biomass pulps	36:68	unbleached oil palm biomass pulps for dermal drug delivery	36:93	Cellulose hydrogel development from unbleached oil palm biomass pulps for dermal drug delivery.					
36273545	5	89	from	solvents	686:693	arg1	solubilised					664:674	solubilised	664:674	solubilised	664:674	Unbleached dissolving pulps were solubilised in alkali solvents containing sodium hydroxide (NaOH) (6-8%w/v) and urea (4-6%w/v) before crosslinking.					
36273545	6	90	theme	permeation	826:835	arg1	studies					837:843	skin permeation studies	821:843	skin permeation studies	821:843	Hydrogels were loaded with ibuprofen for skin permeation studies.					
36273545	8	91	theme	NaOH	948:951	arg1	content					953:959	NaOH content	948:959	NaOH content	948:959	NaOH content has a dominant role over urea where thinner and deformable crosslinked network walls in a porous hydrogel structure are associated with high NaOH content.					
36273545	9	92	theme	swelling	1165:1172	arg1	ratio					1174:1178	swelling ratio	1165:1178	cellulose solubility: 94 %; swelling ratio: ~2800 %	1137:1187	Synergistic effects (cellulose solubility: 94 %; swelling ratio: ~2800 %) were observed at 7%w/v NaOH and 4%w/v urea with low toxicity.					
36539955	0	0	theme	Slow	91:94	arg1	Release					96:102	Slow Release	91:102	Slow Release	91:102	Pomegranate Resembling Design of Starch Sago Beads Encapsulates Nanopyriproxyfen, Enabling Slow Release and Improved Bioactivity.					
36539955	7	1	theme	eggs	1257:1260	arg1	hydrocarbons					1241:1252	the surface hydrocarbons	1229:1252	the surface hydrocarbons of eggs and larvae	1229:1271	It affects the integument of larvae and alters the surface hydrocarbons of eggs and larvae.					
36539955	5	2	theme	physical	934:941	arg1	absorption					943:952	physical absorption	934:952	physical absorption	934:952	The microscopic beads accumulate chitosan-PPX-nanomicelles cross-linked with tripolyphosphate via physical absorption, offering adequate room for water absorption and subsequent PPX release.					
36539955	1	3	theme	disturbing	268:277	arg1	populations					290:300	disturbing non-target populations	268:300	disturbing non-target populations	268:300	Environmental contamination by intense insecticide usage is consistently proposed as a significant contributor to major hazards; further, disturbing non-target populations provoke a grave concern worldwide as they play essential roles in ecosystems.					
36539955	1	4	theme	non-target	279:288	arg1	populations					290:300	disturbing non-target populations	268:300	disturbing non-target populations	268:300	Environmental contamination by intense insecticide usage is consistently proposed as a significant contributor to major hazards; further, disturbing non-target populations provoke a grave concern worldwide as they play essential roles in ecosystems.					
36539955	2	5	theme	water	529:533	arg1	concentrations					535:548	water concentrations	529:548	water concentrations that exceed regulatory pollution thresholds	529:592	Pyriproxyfen is one of the most widely used pesticides; however, due to its probable toxicity, its global application in large amounts may result in water concentrations that exceed regulatory pollution thresholds.					
36539955	4	6	theme	sago	766:769	arg1	beads					771:775	sago beads	766:775	sago beads	766:775	Our design is inspired by the composite structure of sago beads, composed of several small beads resembling a pomegranate.					
36539955	4	7	theme	composite	743:751	arg1	structure					753:761	the composite structure	739:761	the composite structure	739:761	Our design is inspired by the composite structure of sago beads, composed of several small beads resembling a pomegranate.					
36539955	7	8	theme	surface	1233:1239	arg1	hydrocarbons					1241:1252	the surface hydrocarbons	1229:1252	the surface hydrocarbons of eggs and larvae	1229:1271	It affects the integument of larvae and alters the surface hydrocarbons of eggs and larvae.					
36539955	5	9	theme	PPX	1014:1016	arg1	release					1018:1024	subsequent PPX release	1003:1024	subsequent PPX release	1003:1024	The microscopic beads accumulate chitosan-PPX-nanomicelles cross-linked with tripolyphosphate via physical absorption, offering adequate room for water absorption and subsequent PPX release.					
36539955	4	10	theme	small	798:802	arg1	beads					804:808	several small beads	790:808	several small beads resembling a pomegranate	790:833	Our design is inspired by the composite structure of sago beads, composed of several small beads resembling a pomegranate.					
36539955	7	11	theme	larvae	1211:1216	arg1	integument					1197:1206	the integument	1193:1206	the integument of larvae	1193:1216	It affects the integument of larvae and alters the surface hydrocarbons of eggs and larvae.					
36539955	6	12	theme	embryonic	1119:1127	arg1	development					1129:1139	embryonic development	1119:1139	embryonic development in the eggs	1119:1151	PPX-NCB had distinct effects on the immature egg and larva of Anopheles stephensi, limiting embryonic development in the eggs while enhancing bioactivity.					
36539955	2	13	theme	used	419:422	arg1	pesticides					424:433	the most widely used pesticides	403:433	the most widely used pesticides	403:433	Pyriproxyfen is one of the most widely used pesticides; however, due to its probable toxicity, its global application in large amounts may result in water concentrations that exceed regulatory pollution thresholds.					
36539955	0	14	theme	Improved	108:115	arg1	Bioactivity					117:127	Improved Bioactivity	108:127	Improved Bioactivity	108:127	Pomegranate Resembling Design of Starch Sago Beads Encapsulates Nanopyriproxyfen, Enabling Slow Release and Improved Bioactivity.					
36539955	2	15	theme	global	479:484	arg1	application					486:496	its global application	475:496	its global application in large amounts	475:513	Pyriproxyfen is one of the most widely used pesticides; however, due to its probable toxicity, its global application in large amounts may result in water concentrations that exceed regulatory pollution thresholds.					
36539955	4	16	theme	beads	771:775	arg1	structure					753:761	the composite structure	739:761	the composite structure	739:761	Our design is inspired by the composite structure of sago beads, composed of several small beads resembling a pomegranate.					
36539955	2	17	theme	probable	456:463	arg1	toxicity					465:472	its probable toxicity	452:472	its probable toxicity	452:472	Pyriproxyfen is one of the most widely used pesticides; however, due to its probable toxicity, its global application in large amounts may result in water concentrations that exceed regulatory pollution thresholds.					
36539955	6	18	from	effects	1048:1054	arg1	larva					1080:1084	larva	1080:1084	larva	1080:1084	PPX-NCB had distinct effects on the immature egg and larva of Anopheles stephensi, limiting embryonic development in the eggs while enhancing bioactivity.					
36539955	6	18	from	effects	1048:1054	arg1	egg					1072:1074	the immature egg	1059:1074	the immature egg	1059:1074	PPX-NCB had distinct effects on the immature egg and larva of Anopheles stephensi, limiting embryonic development in the eggs while enhancing bioactivity.					
36539955	6	19	from	development	1129:1139	arg1	eggs					1148:1151	the eggs	1144:1151	the eggs	1144:1151	PPX-NCB had distinct effects on the immature egg and larva of Anopheles stephensi, limiting embryonic development in the eggs while enhancing bioactivity.					
36539955	5	20	theme	water	982:986	arg1	absorption					988:997	water absorption	982:997	water absorption	982:997	The microscopic beads accumulate chitosan-PPX-nanomicelles cross-linked with tripolyphosphate via physical absorption, offering adequate room for water absorption and subsequent PPX release.					
36539955	6	21	theme	stephensi	1099:1107	arg1	effects					1048:1054	distinct effects	1039:1054	distinct effects on the immature egg and larva of Anopheles stephensi	1039:1107	PPX-NCB had distinct effects on the immature egg and larva of Anopheles stephensi, limiting embryonic development in the eggs while enhancing bioactivity.					
36539955	1	22	theme	grave	312:316	arg1	worldwide					326:334	a grave concern worldwide	310:334	a grave concern worldwide	310:334	Environmental contamination by intense insecticide usage is consistently proposed as a significant contributor to major hazards; further, disturbing non-target populations provoke a grave concern worldwide as they play essential roles in ecosystems.					
36539955	2	23	theme	regulatory	562:571	arg1	thresholds					583:592	regulatory pollution thresholds	562:592	regulatory pollution thresholds	562:592	Pyriproxyfen is one of the most widely used pesticides; however, due to its probable toxicity, its global application in large amounts may result in water concentrations that exceed regulatory pollution thresholds.					
36539955	8	24	from	profile	1327:1333	arg1	magna					1357:1361	non-target Daphnia magna	1338:1361	non-target Daphnia magna	1338:1361	In addition, PPX-NCB demonstrates an improved safety profile in non-target Daphnia magna.					
36539955	1	25	theme	significant	217:227	arg1	contamination					144:156	Environmental contamination	130:156	Environmental contamination by intense insecticide usage	130:185	Environmental contamination by intense insecticide usage is consistently proposed as a significant contributor to major hazards; further, disturbing non-target populations provoke a grave concern worldwide as they play essential roles in ecosystems.					
36539955	1	25	theme	significant	217:227	arg1	contributor					229:239	a significant contributor	215:239	a significant contributor to major hazards	215:256	Environmental contamination by intense insecticide usage is consistently proposed as a significant contributor to major hazards; further, disturbing non-target populations provoke a grave concern worldwide as they play essential roles in ecosystems.					
36539955	6	26	contain	had	1035:1037	arg1	PPX-NCB					1027:1033	PPX-NCB	1027:1033	PPX-NCB	1027:1033	PPX-NCB had distinct effects on the immature egg and larva of Anopheles stephensi, limiting embryonic development in the eggs while enhancing bioactivity.					
36539955	6	26	contain	had	1035:1037	arg2	effects					1048:1054	distinct effects	1039:1054	distinct effects on the immature egg and larva of Anopheles stephensi	1039:1107	PPX-NCB had distinct effects on the immature egg and larva of Anopheles stephensi, limiting embryonic development in the eggs while enhancing bioactivity.					
36539955	1	27	theme	concern	318:324	arg1	worldwide					326:334	a grave concern worldwide	310:334	a grave concern worldwide	310:334	Environmental contamination by intense insecticide usage is consistently proposed as a significant contributor to major hazards; further, disturbing non-target populations provoke a grave concern worldwide as they play essential roles in ecosystems.					
36539955	0	28	dep	Nanopyriproxyfen	64:79	arg1	Pomegranate					0:10	Pomegranate	0:10	Pomegranate Resembling Design of Starch Sago Beads	0:49	Pomegranate Resembling Design of Starch Sago Beads Encapsulates Nanopyriproxyfen, Enabling Slow Release and Improved Bioactivity.					
36539955	0	29	theme	Sago	40:43	arg1	Beads					45:49	Starch Sago Beads	33:49	Starch Sago Beads	33:49	Pomegranate Resembling Design of Starch Sago Beads Encapsulates Nanopyriproxyfen, Enabling Slow Release and Improved Bioactivity.					
36539955	2	30	from	application	486:496	arg1	amounts					507:513	large amounts	501:513	large amounts	501:513	Pyriproxyfen is one of the most widely used pesticides; however, due to its probable toxicity, its global application in large amounts may result in water concentrations that exceed regulatory pollution thresholds.					
36539955	8	31	theme	Daphnia	1349:1355	arg1	magna					1357:1361	non-target Daphnia magna	1338:1361	non-target Daphnia magna	1338:1361	In addition, PPX-NCB demonstrates an improved safety profile in non-target Daphnia magna.					
36539955	5	32	theme	microscopic	840:850	arg1	beads					852:856	The microscopic beads	836:856	The microscopic beads	836:856	The microscopic beads accumulate chitosan-PPX-nanomicelles cross-linked with tripolyphosphate via physical absorption, offering adequate room for water absorption and subsequent PPX release.					
36539955	0	33	theme	Starch	33:38	arg1	Beads					45:49	Starch Sago Beads	33:49	Starch Sago Beads	33:49	Pomegranate Resembling Design of Starch Sago Beads Encapsulates Nanopyriproxyfen, Enabling Slow Release and Improved Bioactivity.					
36539955	6	34	theme	distinct	1039:1046	arg1	effects					1048:1054	distinct effects	1039:1054	distinct effects on the immature egg and larva of Anopheles stephensi	1039:1107	PPX-NCB had distinct effects on the immature egg and larva of Anopheles stephensi, limiting embryonic development in the eggs while enhancing bioactivity.					
36539955	8	35	theme	safety	1320:1325	arg1	profile					1327:1333	an improved safety profile	1308:1333	an improved safety profile in non-target Daphnia magna	1308:1361	In addition, PPX-NCB demonstrates an improved safety profile in non-target Daphnia magna.					
36539955	3	36	theme	nanopyriproxyfen-loaded	615:637	arg1	beads					644:648	nanopyriproxyfen-loaded sago beads	615:648	nanopyriproxyfen-loaded sago beads (PPX-NCB) designed for the slow release of pyriproxyfen (PPX)	615:710	Herein, we describe nanopyriproxyfen-loaded sago beads (PPX-NCB) designed for the slow release of pyriproxyfen (PPX).					
36539955	3	36	theme	nanopyriproxyfen-loaded	615:637	arg1	PPX-NCB					651:657	PPX-NCB	651:657	PPX-NCB	651:657	Herein, we describe nanopyriproxyfen-loaded sago beads (PPX-NCB) designed for the slow release of pyriproxyfen (PPX).					
36539955	1	37	theme	major	244:248	arg1	hazards					250:256	major hazards	244:256	major hazards	244:256	Environmental contamination by intense insecticide usage is consistently proposed as a significant contributor to major hazards; further, disturbing non-target populations provoke a grave concern worldwide as they play essential roles in ecosystems.					
36539955	0	38	theme	Beads	45:49	arg1	Design					23:28	Design	23:28	Design of Starch Sago Beads	23:49	Pomegranate Resembling Design of Starch Sago Beads Encapsulates Nanopyriproxyfen, Enabling Slow Release and Improved Bioactivity.					
36539955	1	39	theme	Environmental	130:142	arg1	contamination					144:156	Environmental contamination	130:156	Environmental contamination by intense insecticide usage	130:185	Environmental contamination by intense insecticide usage is consistently proposed as a significant contributor to major hazards; further, disturbing non-target populations provoke a grave concern worldwide as they play essential roles in ecosystems.					
36539955	1	39	theme	Environmental	130:142	arg1	contributor					229:239	a significant contributor	215:239	a significant contributor to major hazards	215:256	Environmental contamination by intense insecticide usage is consistently proposed as a significant contributor to major hazards; further, disturbing non-target populations provoke a grave concern worldwide as they play essential roles in ecosystems.					
36539955	1	40	theme	essential	349:357	arg1	roles					359:363	essential roles	349:363	essential roles	349:363	Environmental contamination by intense insecticide usage is consistently proposed as a significant contributor to major hazards; further, disturbing non-target populations provoke a grave concern worldwide as they play essential roles in ecosystems.					
36539955	3	41	theme	pyriproxyfen	693:704	arg1	release					682:688	the slow release	673:688	the slow release of pyriproxyfen (PPX)	673:710	Herein, we describe nanopyriproxyfen-loaded sago beads (PPX-NCB) designed for the slow release of pyriproxyfen (PPX).					
36539955	5	42	theme	adequate	964:971	arg1	room					973:976	adequate room	964:976	adequate room for water absorption and subsequent PPX release	964:1024	The microscopic beads accumulate chitosan-PPX-nanomicelles cross-linked with tripolyphosphate via physical absorption, offering adequate room for water absorption and subsequent PPX release.					
36539955	8	43	theme	improved	1311:1318	arg1	profile					1327:1333	an improved safety profile	1308:1333	an improved safety profile in non-target Daphnia magna	1308:1361	In addition, PPX-NCB demonstrates an improved safety profile in non-target Daphnia magna.					
36539955	2	44	theme	pollution	573:581	arg1	thresholds					583:592	regulatory pollution thresholds	562:592	regulatory pollution thresholds	562:592	Pyriproxyfen is one of the most widely used pesticides; however, due to its probable toxicity, its global application in large amounts may result in water concentrations that exceed regulatory pollution thresholds.					
36539955	3	45	theme	slow	677:680	arg1	release					682:688	the slow release	673:688	the slow release of pyriproxyfen (PPX)	673:710	Herein, we describe nanopyriproxyfen-loaded sago beads (PPX-NCB) designed for the slow release of pyriproxyfen (PPX).					
36539955	5	46	theme	subsequent	1003:1012	arg1	release					1018:1024	subsequent PPX release	1003:1024	subsequent PPX release	1003:1024	The microscopic beads accumulate chitosan-PPX-nanomicelles cross-linked with tripolyphosphate via physical absorption, offering adequate room for water absorption and subsequent PPX release.					
36539955	8	47	theme	non-target	1338:1347	arg1	magna					1357:1361	non-target Daphnia magna	1338:1361	non-target Daphnia magna	1338:1361	In addition, PPX-NCB demonstrates an improved safety profile in non-target Daphnia magna.					
36539955	6	48	theme	immature	1063:1070	arg1	egg					1072:1074	the immature egg	1059:1074	the immature egg	1059:1074	PPX-NCB had distinct effects on the immature egg and larva of Anopheles stephensi, limiting embryonic development in the eggs while enhancing bioactivity.					
36539955	7	49	theme	larvae	1266:1271	arg1	hydrocarbons					1241:1252	the surface hydrocarbons	1229:1252	the surface hydrocarbons of eggs and larvae	1229:1271	It affects the integument of larvae and alters the surface hydrocarbons of eggs and larvae.					
36539955	2	50	theme	pesticides	424:433	arg1	one					396:398	one	396:398	one	396:398	Pyriproxyfen is one of the most widely used pesticides; however, due to its probable toxicity, its global application in large amounts may result in water concentrations that exceed regulatory pollution thresholds.					
36539955	2	50	theme	pesticides	424:433	arg1	pesticides					424:433	the most widely used pesticides	403:433	the most widely used pesticides	403:433	Pyriproxyfen is one of the most widely used pesticides; however, due to its probable toxicity, its global application in large amounts may result in water concentrations that exceed regulatory pollution thresholds.					
36539955	3	51	theme	sago	639:642	arg1	beads					644:648	nanopyriproxyfen-loaded sago beads	615:648	nanopyriproxyfen-loaded sago beads (PPX-NCB) designed for the slow release of pyriproxyfen (PPX)	615:710	Herein, we describe nanopyriproxyfen-loaded sago beads (PPX-NCB) designed for the slow release of pyriproxyfen (PPX).					
36539955	3	51	theme	sago	639:642	arg1	PPX-NCB					651:657	PPX-NCB	651:657	PPX-NCB	651:657	Herein, we describe nanopyriproxyfen-loaded sago beads (PPX-NCB) designed for the slow release of pyriproxyfen (PPX).					
36539955	6	52	theme	Anopheles	1089:1097	arg1	stephensi					1099:1107	Anopheles stephensi	1089:1107	Anopheles stephensi	1089:1107	PPX-NCB had distinct effects on the immature egg and larva of Anopheles stephensi, limiting embryonic development in the eggs while enhancing bioactivity.					
36539955	1	53	theme	intense	161:167	arg1	usage					181:185	intense insecticide usage	161:185	intense insecticide usage	161:185	Environmental contamination by intense insecticide usage is consistently proposed as a significant contributor to major hazards; further, disturbing non-target populations provoke a grave concern worldwide as they play essential roles in ecosystems.					
36539955	2	54	theme	large	501:505	arg1	amounts					507:513	large amounts	501:513	large amounts	501:513	Pyriproxyfen is one of the most widely used pesticides; however, due to its probable toxicity, its global application in large amounts may result in water concentrations that exceed regulatory pollution thresholds.					
36539955	4	55	theme	several	790:796	arg1	beads					804:808	several small beads	790:808	several small beads resembling a pomegranate	790:833	Our design is inspired by the composite structure of sago beads, composed of several small beads resembling a pomegranate.					
36539955	1	56	theme	insecticide	169:179	arg1	usage					181:185	intense insecticide usage	161:185	intense insecticide usage	161:185	Environmental contamination by intense insecticide usage is consistently proposed as a significant contributor to major hazards; further, disturbing non-target populations provoke a grave concern worldwide as they play essential roles in ecosystems.					
35913200	4	0	theme	negative	673:680	arg1	groups					691:696	the negative carboxyl groups	669:696	the negative carboxyl groups from sodium hyaluronate	669:720	Because of the negative carboxyl groups from sodium hyaluronate, the top surface possessed a friction coefficient as low as 0.024.					
35913200	1	1	theme	low	151:153	arg1	coefficient					164:174	low friction coefficient	151:174	low friction coefficient	151:174	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	1	2	theme	articular	263:271	arg1	cartilage					273:281	replacing articular cartilage	253:281	replacing articular cartilage	253:281	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	0	3	theme	Cartilage	64:72	arg1	Structure					28:36	Structure	28:36	Structure	28:36	Janus Hydrogel to Mimic the Structure and Property of Articular Cartilage.					
35913200	0	3	theme	Cartilage	64:72	arg1	Property					42:49	Property	42:49	Property	42:49	Janus Hydrogel to Mimic the Structure and Property of Articular Cartilage.					
35913200	7	4	theme	PBS	1096:1098	arg1	solution					1100:1107	PBS solution	1096:1107	PBS solution	1096:1107	Furthermore, it demonstrated remarkable swelling resistance with strength retention near 100% even after soaking in PBS solution at 37 °C for 7 days.					
35913200	3	5	from	dipping	495:501	arg1	mineralization					525:538	situ precipitation mineralization	506:538	situ precipitation mineralization	506:538	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	1	6	theme	friction	155:162	arg1	coefficient					164:174	low friction coefficient	151:174	low friction coefficient	151:174	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	4	7	theme	friction	751:758	arg1	coefficient					760:770	a friction coefficient	749:770	a friction coefficient as low as 0.024	749:786	Because of the negative carboxyl groups from sodium hyaluronate, the top surface possessed a friction coefficient as low as 0.024.					
35913200	8	8	theme	articular	1306:1314	arg1	cartilage					1316:1324	articular cartilage	1306:1324	articular cartilage	1306:1324	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	3	9	theme	hybridized	559:568	arg1	HAp					586:588	HAp	586:588	HAp	586:588	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	3	9	theme	hybridized	559:568	arg1	wherein					592:598	wherein the two surfaces had distinct compositions and functions	592:655	wherein the two surfaces had distinct compositions and functions	592:655	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	3	9	theme	hybridized	559:568	arg1	hydroxyapatite					570:583	hybridized hydroxyapatite	559:583	hybridized hydroxyapatite (HAp)	559:589	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	8	10	theme	viability	1253:1261	arg1	%					1268:1268	cell viability ≈ 100%	1248:1268	cell viability ≈ 100%	1248:1268	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	8	10	theme	viability	1253:1261	arg1	biocompatibility					1230:1245	excellent biocompatibility	1220:1245	excellent biocompatibility (cell viability ≈ 100%)	1220:1269	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	4	11	theme	carboxyl	682:689	arg1	groups					691:696	the negative carboxyl groups	669:696	the negative carboxyl groups from sodium hyaluronate	669:720	Because of the negative carboxyl groups from sodium hyaluronate, the top surface possessed a friction coefficient as low as 0.024.					
35913200	8	12	theme	cell	1248:1251	arg1	%					1268:1268	cell viability ≈ 100%	1248:1268	cell viability ≈ 100%	1248:1268	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	8	12	theme	cell	1248:1251	arg1	biocompatibility					1230:1245	excellent biocompatibility	1220:1245	excellent biocompatibility (cell viability ≈ 100%)	1220:1269	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	5	13	theme	mineralized	811:821	arg1	layer					823:827	the HAp mineralized layer	803:827	the HAp mineralized layer	803:827	On account of the HAp mineralized layer, the bottom side had osteogenesis potential.					
35913200	4	14	theme	top	727:729	arg1	surface					731:737	the top surface	723:737	the top surface	723:737	Because of the negative carboxyl groups from sodium hyaluronate, the top surface possessed a friction coefficient as low as 0.024.					
35913200	8	15	theme	starting	1186:1193	arg1	polymers					1195:1202	starting polymers	1186:1202	starting polymers	1186:1202	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	6	16	theme	compressive	940:950	arg1	strength					952:959	compressive strength	940:959	compressive strength	940:959	Owing to the synergy of physical linkages, the hydrogel displayed compressive strength as high as 78 MPa.					
35913200	7	17	with	resistance	1029:1038	arg1	retention					1054:1062	strength retention	1045:1062	strength retention	1045:1062	Furthermore, it demonstrated remarkable swelling resistance with strength retention near 100% even after soaking in PBS solution at 37 °C for 7 days.					
35913200	5	18	theme	bottom	834:839	arg1	side					841:844	the bottom side	830:844	the bottom side	830:844	On account of the HAp mineralized layer, the bottom side had osteogenesis potential.					
35913200	3	19	theme	Janus	385:389	arg1	hydrogel					391:398	a Janus hydrogel	383:398	a Janus hydrogel	383:398	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	1	20	theme	Designing	75:83	arg1	hydrogels					85:93	Designing hydrogels	75:93	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential	75:234	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	1	21	theme	excellent	177:185	arg1	biocompatibility					187:202	excellent biocompatibility	177:202	excellent biocompatibility	177:202	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	0	22	theme	Janus	0:4	arg1	Hydrogel					6:13	Janus Hydrogel	0:13	Janus Hydrogel	0:13	Janus Hydrogel to Mimic the Structure and Property of Articular Cartilage.					
35913200	8	23	dep	ideal	1285:1289	arg1	substitute					1291:1300	substitute	1291:1300	substitute for articular cartilage	1291:1324	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	3	24	theme	hydroxyapatite	570:583	arg1	layer					550:554	a layer	548:554	a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions	548:655	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	8	25	theme	polymers	1195:1202	arg1	merits					1176:1181	the merits	1172:1181	the merits of starting polymers	1172:1202	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	8	26	theme	toxic	1145:1149	arg1	chemicals					1151:1159	toxic chemicals	1145:1159	toxic chemicals	1145:1159	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	4	27	contain	possessed	739:747	arg1	surface					731:737	the top surface	723:737	the top surface	723:737	Because of the negative carboxyl groups from sodium hyaluronate, the top surface possessed a friction coefficient as low as 0.024.					
35913200	4	27	contain	possessed	739:747	arg2	coefficient					760:770	a friction coefficient	749:770	a friction coefficient as low as 0.024	749:786	Because of the negative carboxyl groups from sodium hyaluronate, the top surface possessed a friction coefficient as low as 0.024.					
35913200	7	28	theme	strength	1045:1052	arg1	retention					1054:1062	strength retention	1045:1062	strength retention	1045:1062	Furthermore, it demonstrated remarkable swelling resistance with strength retention near 100% even after soaking in PBS solution at 37 °C for 7 days.					
35913200	5	29	contain	had	846:848	arg1	side					841:844	the bottom side	830:844	the bottom side	830:844	On account of the HAp mineralized layer, the bottom side had osteogenesis potential.					
35913200	5	29	contain	had	846:848	arg2	potential					863:871	osteogenesis potential	850:871	osteogenesis potential	850:871	On account of the HAp mineralized layer, the bottom side had osteogenesis potential.					
35913200	3	30	dep	wherein	592:598	arg1	had					617:619	had	617:619	had distinct compositions and functions	617:655	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	1	31	with	hydrogels	85:93	arg1	strength					109:116	adequate strength	100:116	adequate strength	100:116	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	1	31	with	hydrogels	85:93	arg1	biocompatibility					187:202	excellent biocompatibility	177:202	excellent biocompatibility	177:202	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	1	31	with	hydrogels	85:93	arg1	coefficient					164:174	low friction coefficient	151:174	low friction coefficient	151:174	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	1	31	with	hydrogels	85:93	arg1	resistance					139:148	remarkable swelling resistance	119:148	remarkable swelling resistance	119:148	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	1	31	with	hydrogels	85:93	arg1	potential					226:234	osseointegration potential	209:234	osseointegration potential	209:234	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	1	32	theme	adequate	100:107	arg1	strength					109:116	adequate strength	100:116	adequate strength	100:116	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	3	33	contain	had	617:619	arg2	compositions					630:641	distinct compositions	621:641	distinct compositions	621:641	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	3	33	contain	had	617:619	arg2	functions					647:655	functions	647:655	functions	647:655	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	3	33	contain	had	617:619	arg1	surfaces					608:615	the two surfaces	600:615	the two surfaces	600:615	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	1	34	theme	osseointegration	209:224	arg1	potential					226:234	osseointegration potential	209:234	osseointegration potential	209:234	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	3	35	theme	polyvinyl	418:426	arg1	alcohol					428:434	polyvinyl alcohol	418:434	polyvinyl alcohol	418:434	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	8	36	theme	chemicals	1151:1159	arg1	absence					1134:1140	The absence	1130:1140	The absence of toxic chemicals	1130:1159	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	3	37	theme	sodium	451:456	arg1	hyaluronate					458:468	sodium hyaluronate	451:468	sodium hyaluronate	451:468	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	5	38	theme	osteogenesis	850:861	arg1	potential					863:871	osteogenesis potential	850:871	osteogenesis potential	850:871	On account of the HAp mineralized layer, the bottom side had osteogenesis potential.					
35913200	7	39	theme	remarkable	1009:1018	arg1	resistance					1029:1038	remarkable swelling resistance	1009:1038	remarkable swelling resistance with strength retention	1009:1062	Furthermore, it demonstrated remarkable swelling resistance with strength retention near 100% even after soaking in PBS solution at 37 °C for 7 days.					
35913200	3	40	theme	distinct	621:628	arg1	compositions					630:641	distinct compositions	621:641	distinct compositions	621:641	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	0	41	dep	Structure	28:36	arg1	the					24:26	the	24:26	the	24:26	Janus Hydrogel to Mimic the Structure and Property of Articular Cartilage.					
35913200	4	42	theme	sodium	703:708	arg1	hyaluronate					710:720	sodium hyaluronate	703:720	sodium hyaluronate	703:720	Because of the negative carboxyl groups from sodium hyaluronate, the top surface possessed a friction coefficient as low as 0.024.					
35913200	6	43	theme	linkages	907:914	arg1	synergy					887:893	the synergy	883:893	the synergy of physical linkages	883:914	Owing to the synergy of physical linkages, the hydrogel displayed compressive strength as high as 78 MPa.					
35913200	7	44	theme	swelling	1020:1027	arg1	resistance					1029:1038	remarkable swelling resistance	1009:1038	remarkable swelling resistance with strength retention	1009:1062	Furthermore, it demonstrated remarkable swelling resistance with strength retention near 100% even after soaking in PBS solution at 37 °C for 7 days.					
35913200	3	45	theme	one-sided	485:493	arg1	dipping					495:501	a one-sided dipping	483:501	a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions	483:655	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	6	46	theme	physical	898:905	arg1	linkages					907:914	physical linkages	898:914	physical linkages	898:914	Owing to the synergy of physical linkages, the hydrogel displayed compressive strength as high as 78 MPa.					
35913200	1	47	theme	remarkable	119:128	arg1	resistance					139:148	remarkable swelling resistance	119:148	remarkable swelling resistance	119:148	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	4	48	from	hyaluronate	710:720	arg1	groups					691:696	the negative carboxyl groups	669:696	the negative carboxyl groups from sodium hyaluronate	669:720	Because of the negative carboxyl groups from sodium hyaluronate, the top surface possessed a friction coefficient as low as 0.024.					
35913200	4	49	theme	low	775:777	arg1	coefficient					760:770	a friction coefficient	749:770	a friction coefficient as low as 0.024	749:786	Because of the negative carboxyl groups from sodium hyaluronate, the top surface possessed a friction coefficient as low as 0.024.					
35913200	1	50	theme	swelling	130:137	arg1	resistance					139:148	remarkable swelling resistance	119:148	remarkable swelling resistance	119:148	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35913200	8	51	theme	≈	1263:1263	arg1	%					1268:1268	cell viability ≈ 100%	1248:1268	cell viability ≈ 100%	1248:1268	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	8	51	theme	≈	1263:1263	arg1	biocompatibility					1230:1245	excellent biocompatibility	1220:1245	excellent biocompatibility (cell viability ≈ 100%)	1220:1269	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	0	52	theme	Articular	54:62	arg1	Cartilage					64:72	Articular Cartilage	54:72	Articular Cartilage	54:72	Janus Hydrogel to Mimic the Structure and Property of Articular Cartilage.					
35913200	8	53	theme	excellent	1220:1228	arg1	%					1268:1268	cell viability ≈ 100%	1248:1268	cell viability ≈ 100%	1248:1268	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	8	53	theme	excellent	1220:1228	arg1	biocompatibility					1230:1245	excellent biocompatibility	1220:1245	excellent biocompatibility (cell viability ≈ 100%)	1220:1269	The absence of toxic chemicals maintained the merits of starting polymers and resulted in excellent biocompatibility (cell viability ≈ 100%), making it an ideal substitute for articular cartilage.					
35913200	5	54	theme	HAp	807:809	arg1	layer					823:827	the HAp mineralized layer	803:827	the HAp mineralized layer	803:827	On account of the HAp mineralized layer, the bottom side had osteogenesis potential.					
35913200	3	55	theme	situ	506:509	arg1	mineralization					525:538	situ precipitation mineralization	506:538	situ precipitation mineralization	506:538	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	3	56	theme	precipitation	511:523	arg1	mineralization					525:538	situ precipitation mineralization	506:538	situ precipitation mineralization	506:538	In this work, a Janus hydrogel was prepared from polyvinyl alcohol, chitosan, and sodium hyaluronate, followed by a one-sided dipping in situ precipitation mineralization to form a layer of hybridized hydroxyapatite (HAp), wherein the two surfaces had distinct compositions and functions.					
35913200	1	57	theme	replacing	253:261	arg1	cartilage					273:281	replacing articular cartilage	253:281	replacing articular cartilage	253:281	Designing hydrogels with adequate strength, remarkable swelling resistance, low friction coefficient, excellent biocompatibility, and osseointegration potential is essential for replacing articular cartilage.					
35131354	9	0	theme	glass-transition	1390:1405	arg1	values					1424:1429	glass-transition temperature (Tg) values	1390:1429	glass-transition temperature (Tg) values of HME-filaments	1390:1446	Experimental feedability was predicted using texture analysis results was a function of PEG concentration, and glass-transition temperature (Tg) values of HME-filaments.					
35131354	14	1	theme	layers	2174:2179	arg1	consistency					2151:2161	thickness consistency	2141:2161	thickness consistency of printed layers (using homogeneous HME-filaments)	2141:2213	The plasticization of HPMCAS and the addition of talc into the formulation were shown to improve thickness consistency of printed layers (using homogeneous HME-filaments).					
35131354	13	2	theme	high	1937:1940	arg1	restriction					2010:2020	an additional restriction	1996:2020	an additional restriction for nozzle extrusion	1996:2041	The high viscosity of unplasticized HPMCAS was concluded to be an additional restriction for nozzle extrusion.					
35131354	13	2	theme	high	1937:1940	arg1	viscosity					1942:1950	The high viscosity	1933:1950	The high viscosity of unplasticized HPMCAS	1933:1974	The high viscosity of unplasticized HPMCAS was concluded to be an additional restriction for nozzle extrusion.					
35131354	1	3	theme	methods	228:234	arg1	one					190:192	one	190:192	one	190:192	3D printing (3DP) by fused deposition modelling (FDM) is one of the most extensively developed methods in additive manufacturing.					
35131354	1	3	theme	methods	228:234	arg1	methods					228:234	the most extensively developed methods	197:234	the most extensively developed methods in additive manufacturing	197:260	3D printing (3DP) by fused deposition modelling (FDM) is one of the most extensively developed methods in additive manufacturing.					
35131354	4	4	theme	fabrication	624:634	arg1	effect					585:590	the effect	581:590	the effect of HME-filament composition and fabrication on printability	581:650	It explored and demonstrated the effect of HME-filament composition and fabrication on printability by evaluating thermal, mechanical, and thermo-rheological properties.					
35131354	10	5	theme	HME-filament	1523:1534	arg1	inhomogeneity					1506:1518	inhomogeneity	1506:1518	inhomogeneity	1506:1518	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	5	6	theme	tailored	845:852	arg1	profile					867:873	a tailored drug release profile	843:873	a tailored drug release profile	843:873	It also showed that the HME-Polymer filament composition used in FDM-3DP manufacture of oral solid dosage forms provides a tailored drug release profile.					
35131354	8	7	theme	insoluble	1222:1230	arg1	agent					1244:1248	an insoluble structuring agent	1219:1248	an insoluble structuring agent (talc) into the formulation	1219:1276	These include plasticizing the polymer and adding an insoluble structuring agent (talc) into the formulation.					
35131354	8	7	theme	insoluble	1222:1230	arg1	talc					1251:1254	talc	1251:1254	talc	1251:1254	These include plasticizing the polymer and adding an insoluble structuring agent (talc) into the formulation.					
35131354	14	8	theme	thickness	2141:2149	arg1	consistency					2151:2161	thickness consistency	2141:2161	thickness consistency of printed layers (using homogeneous HME-filaments)	2141:2213	The plasticization of HPMCAS and the addition of talc into the formulation were shown to improve thickness consistency of printed layers (using homogeneous HME-filaments).					
35131354	11	9	theme	extrudates	1763:1772	arg1	images					1746:1751	scanning electron microscopy (SEM) images	1711:1751	scanning electron microscopy (SEM) images of nozzle extrudates	1711:1772	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	1	10	theme	deposition	160:169	arg1	FDM					182:184	FDM	182:184	FDM	182:184	3D printing (3DP) by fused deposition modelling (FDM) is one of the most extensively developed methods in additive manufacturing.					
35131354	1	10	theme	deposition	160:169	arg1	modelling					171:179	fused deposition modelling	154:179	fused deposition modelling (FDM)	154:185	3D printing (3DP) by fused deposition modelling (FDM) is one of the most extensively developed methods in additive manufacturing.					
35131354	1	11	from	methods	228:234	arg1	manufacturing					248:260	additive manufacturing	239:260	additive manufacturing	239:260	3D printing (3DP) by fused deposition modelling (FDM) is one of the most extensively developed methods in additive manufacturing.					
35131354	3	12	theme	hot-melt	526:533	arg1	HME					546:548	HME	546:548	HME	546:548	This work aimed to use HPMCAS (AffinisolTM HPMCAS 716) to prepare filaments for FDM-3DP using hot-melt extrusion (HME).					
35131354	3	12	theme	hot-melt	526:533	arg1	extrusion					535:543	hot-melt extrusion	526:543	hot-melt extrusion (HME)	526:549	This work aimed to use HPMCAS (AffinisolTM HPMCAS 716) to prepare filaments for FDM-3DP using hot-melt extrusion (HME).					
35131354	5	13	theme	dosage	821:826	arg1	forms					828:832	oral solid dosage forms	810:832	oral solid dosage forms	810:832	It also showed that the HME-Polymer filament composition used in FDM-3DP manufacture of oral solid dosage forms provides a tailored drug release profile.					
35131354	4	14	theme	composition	608:618	arg1	effect					585:590	the effect	581:590	the effect of HME-filament composition and fabrication on printability	581:650	It explored and demonstrated the effect of HME-filament composition and fabrication on printability by evaluating thermal, mechanical, and thermo-rheological properties.					
35131354	10	15	theme	extrudate	1591:1599	arg1	inconsistency					1555:1567	inconsistency	1555:1567	inconsistency of the printer nozzle extrudate and printed layers	1555:1618	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	11	16	theme	wall	1790:1793	arg1	variability					1625:1635	The variability	1621:1635	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet	1621:1815	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	7	17	theme	mechanical	1023:1032	arg1	properties					1034:1043	the mechanical properties	1019:1043	the mechanical properties of HME-filaments	1019:1060	Two diverse ways of improving the mechanical properties of HME-filaments were deduced by changing the formulation to enable feeding through the roller gears of the printer nozzle.					
35131354	0	18	theme	pharmaceutical	15:28	arg1	forms					48:52	pharmaceutical oral solid dosage forms	15:52	pharmaceutical oral solid dosage forms	15:52	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.					
35131354	10	19	theme	printer	1576:1582	arg1	extrudate					1591:1599	the printer nozzle extrudate	1572:1599	the printer nozzle extrudate	1572:1599	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	6	20	used	used	924:927	arg2	FDM-3DP					900:906	FDM-3DP	900:906	FDM-3DP (MakerBot)	900:917	HME (HAAKE MiniLab) and FDM-3DP (MakerBot) were used to prepare HME-filaments and printed objects, respectively.					
35131354	6	20	used	used	924:927	arg2	MiniLab					887:893	MiniLab	887:893	MiniLab	887:893	HME (HAAKE MiniLab) and FDM-3DP (MakerBot) were used to prepare HME-filaments and printed objects, respectively.					
35131354	6	20	used	used	924:927	arg2	MakerBot					909:916	MakerBot	909:916	MakerBot	909:916	HME (HAAKE MiniLab) and FDM-3DP (MakerBot) were used to prepare HME-filaments and printed objects, respectively.					
35131354	6	20	used	used	924:927	arg2	HME					876:878	HME	876:878	HME (HAAKE MiniLab)	876:894	HME (HAAKE MiniLab) and FDM-3DP (MakerBot) were used to prepare HME-filaments and printed objects, respectively.					
35131354	4	21	theme	thermo-rheological	691:708	arg1	properties					710:719	thermal, mechanical, and thermo-rheological properties	666:719	thermal, mechanical, and thermo-rheological properties	666:719	It explored and demonstrated the effect of HME-filament composition and fabrication on printability by evaluating thermal, mechanical, and thermo-rheological properties.					
35131354	11	22	theme	electron	1720:1727	arg1	SEM					1741:1743	SEM	1741:1743	SEM	1741:1743	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	11	22	theme	electron	1720:1727	arg1	microscopy					1729:1738	scanning electron microscopy	1711:1738	scanning electron microscopy (SEM) images of nozzle extrudates	1711:1772	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	9	23	theme	temperature	1407:1417	arg1	values					1424:1429	glass-transition temperature (Tg) values	1390:1429	glass-transition temperature (Tg) values of HME-filaments	1390:1446	Experimental feedability was predicted using texture analysis results was a function of PEG concentration, and glass-transition temperature (Tg) values of HME-filaments.					
35131354	15	24	theme	solidification	2261:2274	arg1	threshold					2276:2284	the solidification threshold	2257:2284	the solidification threshold (low-frequency oscillation test determined by parallel-plate rheometer)	2257:2356	A good correlation (R2 = 0.9546) between the solidification threshold (low-frequency oscillation test determined by parallel-plate rheometer) and Tg of HME-filaments was also established.					
35131354	5	25	theme	HME-Polymer	746:756	arg1	composition					767:777	the HME-Polymer filament composition	742:777	the HME-Polymer filament composition used in FDM-3DP manufacture of oral solid dosage forms	742:832	It also showed that the HME-Polymer filament composition used in FDM-3DP manufacture of oral solid dosage forms provides a tailored drug release profile.					
35131354	12	26	theme	HPMCAS	1867:1872	arg1	formulations					1874:1885	HPMCAS formulations	1867:1885	HPMCAS formulations	1867:1885	The melt viscosity of HPMCAS formulations was investigated using a capillary rheometer.					
35131354	6	27	theme	printed	958:964	arg1	objects					966:972	printed objects	958:972	printed objects	958:972	HME (HAAKE MiniLab) and FDM-3DP (MakerBot) were used to prepare HME-filaments and printed objects, respectively.					
35131354	13	28	theme	additional	1999:2008	arg1	restriction					2010:2020	an additional restriction	1996:2020	an additional restriction for nozzle extrusion	1996:2041	The high viscosity of unplasticized HPMCAS was concluded to be an additional restriction for nozzle extrusion.					
35131354	13	28	theme	additional	1999:2008	arg1	viscosity					1942:1950	The high viscosity	1933:1950	The high viscosity of unplasticized HPMCAS	1933:1974	The high viscosity of unplasticized HPMCAS was concluded to be an additional restriction for nozzle extrusion.					
35131354	7	29	theme	nozzle	1161:1166	arg1	gears					1140:1144	the roller gears	1129:1144	the roller gears of the printer nozzle	1129:1166	Two diverse ways of improving the mechanical properties of HME-filaments were deduced by changing the formulation to enable feeding through the roller gears of the printer nozzle.					
35131354	0	30	theme	solid	35:39	arg1	forms					48:52	pharmaceutical oral solid dosage forms	15:52	pharmaceutical oral solid dosage forms	15:52	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.					
35131354	14	31	theme	HPMCAS	2066:2071	arg1	plasticization					2048:2061	The plasticization	2044:2061	The plasticization of HPMCAS and the addition of talc into the formulation	2044:2117	The plasticization of HPMCAS and the addition of talc into the formulation were shown to improve thickness consistency of printed layers (using homogeneous HME-filaments).					
35131354	10	32	theme	layers	1613:1618	arg1	inconsistency					1555:1567	inconsistency	1555:1567	inconsistency of the printer nozzle extrudate and printed layers	1555:1618	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	0	33	theme	3D	0:1	arg1	printing					3:10	3D printing	0:10	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.	0:131	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.					
35131354	2	34	theme	desirable	410:418	arg1	properties					420:429	desirable properties	410:429	desirable properties	410:429	Optimizing printability by improving feedability, nozzle extrusion, and layer deposition is crucial for manufacturing solid oral dosage forms with desirable properties.					
35131354	10	35	theme	screw	1472:1476	arg1	100 rpm					1485:1491	100 rpm	1485:1491	100 rpm	1485:1491	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	10	35	theme	screw	1472:1476	arg1	speed					1478:1482	high HME screw speed	1463:1482	high HME screw speed (100 rpm)	1463:1492	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	5	36	theme	oral	810:813	arg1	forms					828:832	oral solid dosage forms	810:832	oral solid dosage forms	810:832	It also showed that the HME-Polymer filament composition used in FDM-3DP manufacture of oral solid dosage forms provides a tailored drug release profile.					
35131354	9	37	theme	texture	1324:1330	arg1	analysis					1332:1339	texture analysis	1324:1339	texture analysis	1324:1339	Experimental feedability was predicted using texture analysis results was a function of PEG concentration, and glass-transition temperature (Tg) values of HME-filaments.					
35131354	10	38	theme	high	1463:1466	arg1	100 rpm					1485:1491	100 rpm	1485:1491	100 rpm	1485:1491	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	10	38	theme	high	1463:1466	arg1	speed					1478:1482	high HME screw speed	1463:1482	high HME screw speed (100 rpm)	1463:1492	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	2	39	theme	oral	387:390	arg1	forms					399:403	solid oral dosage forms	381:403	solid oral dosage forms	381:403	Optimizing printability by improving feedability, nozzle extrusion, and layer deposition is crucial for manufacturing solid oral dosage forms with desirable properties.					
35131354	0	40	theme	oral	30:33	arg1	forms					48:52	pharmaceutical oral solid dosage forms	15:52	pharmaceutical oral solid dosage forms	15:52	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.					
35131354	15	41	theme	good	2218:2221	arg1	R2 = 0.9546					2236:2246	R2 = 0.9546	2236:2246	R2 = 0.9546	2236:2246	A good correlation (R2 = 0.9546) between the solidification threshold (low-frequency oscillation test determined by parallel-plate rheometer) and Tg of HME-filaments was also established.					
35131354	15	41	theme	good	2218:2221	arg1	correlation					2223:2233	A good correlation	2216:2233	A good correlation (R2 = 0.9546) between the solidification threshold (low-frequency oscillation test determined by parallel-plate rheometer) and Tg of HME-filaments	2216:2380	A good correlation (R2 = 0.9546) between the solidification threshold (low-frequency oscillation test determined by parallel-plate rheometer) and Tg of HME-filaments was also established.					
35131354	11	42	theme	tablet	1810:1815	arg1	Tg					1674:1675	Tg	1674:1675	Tg	1674:1675	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	11	42	theme	tablet	1810:1815	arg1	temperature					1661:1671	the glass-transition temperature	1640:1671	the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates	1640:1772	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	11	42	theme	tablet	1810:1815	arg1	wall					1790:1793	the lateral wall	1778:1793	the lateral wall of the printed tablet	1778:1815	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	0	43	theme	dosage	41:46	arg1	forms					48:52	pharmaceutical oral solid dosage forms	15:52	pharmaceutical oral solid dosage forms	15:52	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.					
35131354	16	44	theme	drug	2528:2531	arg1	profiles					2541:2548	tailored drug release profiles	2519:2548	tailored drug release profiles based on variation of internal geometry (infill)	2519:2597	Drug-loaded and placebo HPMCAS-based formulations were shown to be successfully printed, with the former providing tailored drug release profiles based on variation of internal geometry (infill).					
35131354	15	45	dep	threshold	2276:2284	arg1	determined					2318:2327	determined	2318:2327	determined by parallel-plate rheometer	2318:2355	A good correlation (R2 = 0.9546) between the solidification threshold (low-frequency oscillation test determined by parallel-plate rheometer) and Tg of HME-filaments was also established.					
35131354	13	46	theme	unplasticized	1955:1967	arg1	HPMCAS					1969:1974	unplasticized HPMCAS	1955:1974	unplasticized HPMCAS	1955:1974	The high viscosity of unplasticized HPMCAS was concluded to be an additional restriction for nozzle extrusion.					
35131354	9	47	theme	concentration	1371:1383	arg1	values					1424:1429	glass-transition temperature (Tg) values	1390:1429	glass-transition temperature (Tg) values of HME-filaments	1390:1446	Experimental feedability was predicted using texture analysis results was a function of PEG concentration, and glass-transition temperature (Tg) values of HME-filaments.					
35131354	9	47	theme	concentration	1371:1383	arg1	results					1341:1347	results	1341:1347	results	1341:1347	Experimental feedability was predicted using texture analysis results was a function of PEG concentration, and glass-transition temperature (Tg) values of HME-filaments.					
35131354	9	47	theme	concentration	1371:1383	arg1	function					1355:1362	a function	1353:1362	a function of PEG concentration	1353:1383	Experimental feedability was predicted using texture analysis results was a function of PEG concentration, and glass-transition temperature (Tg) values of HME-filaments.					
35131354	14	48	theme	homogeneous	2188:2198	arg1	HME-filaments					2200:2212	homogeneous HME-filaments	2188:2212	homogeneous HME-filaments	2188:2212	The plasticization of HPMCAS and the addition of talc into the formulation were shown to improve thickness consistency of printed layers (using homogeneous HME-filaments).					
35131354	1	49	theme	developed	218:226	arg1	methods					228:234	the most extensively developed methods	197:234	the most extensively developed methods in additive manufacturing	197:260	3D printing (3DP) by fused deposition modelling (FDM) is one of the most extensively developed methods in additive manufacturing.					
35131354	14	50	theme	printed	2166:2172	arg1	layers					2174:2179	printed layers	2166:2179	printed layers (using homogeneous HME-filaments)	2166:2213	The plasticization of HPMCAS and the addition of talc into the formulation were shown to improve thickness consistency of printed layers (using homogeneous HME-filaments).					
35131354	11	51	theme	glass-transition	1644:1659	arg1	Tg					1674:1675	Tg	1674:1675	Tg	1674:1675	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	11	51	theme	glass-transition	1644:1659	arg1	temperature					1661:1671	the glass-transition temperature	1640:1671	the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates	1640:1772	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	1	52	theme	fused	154:158	arg1	FDM					182:184	FDM	182:184	FDM	182:184	3D printing (3DP) by fused deposition modelling (FDM) is one of the most extensively developed methods in additive manufacturing.					
35131354	1	52	theme	fused	154:158	arg1	modelling					171:179	fused deposition modelling	154:179	fused deposition modelling (FDM)	154:185	3D printing (3DP) by fused deposition modelling (FDM) is one of the most extensively developed methods in additive manufacturing.					
35131354	9	53	theme	Tg	1420:1421	arg1	values					1424:1429	glass-transition temperature (Tg) values	1390:1429	glass-transition temperature (Tg) values of HME-filaments	1390:1446	Experimental feedability was predicted using texture analysis results was a function of PEG concentration, and glass-transition temperature (Tg) values of HME-filaments.					
35131354	7	54	theme	HME-filaments	1048:1060	arg1	properties					1034:1043	the mechanical properties	1019:1043	the mechanical properties of HME-filaments	1019:1060	Two diverse ways of improving the mechanical properties of HME-filaments were deduced by changing the formulation to enable feeding through the roller gears of the printer nozzle.					
35131354	5	55	theme	drug	854:857	arg1	profile					867:873	a tailored drug release profile	843:873	a tailored drug release profile	843:873	It also showed that the HME-Polymer filament composition used in FDM-3DP manufacture of oral solid dosage forms provides a tailored drug release profile.					
35131354	0	56	theme	plasticised	113:123	arg1	HPMCAS					125:130	plasticised HPMCAS	113:130	plasticised HPMCAS	113:130	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.					
35131354	5	57	theme	solid	815:819	arg1	forms					828:832	oral solid dosage forms	810:832	oral solid dosage forms	810:832	It also showed that the HME-Polymer filament composition used in FDM-3DP manufacture of oral solid dosage forms provides a tailored drug release profile.					
35131354	8	58	theme	structuring	1232:1242	arg1	agent					1244:1248	an insoluble structuring agent	1219:1248	an insoluble structuring agent (talc) into the formulation	1219:1276	These include plasticizing the polymer and adding an insoluble structuring agent (talc) into the formulation.					
35131354	8	58	theme	structuring	1232:1242	arg1	talc					1251:1254	talc	1251:1254	talc	1251:1254	These include plasticizing the polymer and adding an insoluble structuring agent (talc) into the formulation.					
35131354	11	59	theme	nozzle	1756:1761	arg1	extrudates					1763:1772	nozzle extrudates	1756:1772	nozzle extrudates	1756:1772	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	15	60	theme	HME-filaments	2368:2380	arg1	threshold					2276:2284	the solidification threshold	2257:2284	the solidification threshold (low-frequency oscillation test determined by parallel-plate rheometer)	2257:2356	A good correlation (R2 = 0.9546) between the solidification threshold (low-frequency oscillation test determined by parallel-plate rheometer) and Tg of HME-filaments was also established.					
35131354	15	60	theme	HME-filaments	2368:2380	arg1	Tg					2362:2363	Tg	2362:2363	Tg of HME-filaments	2362:2380	A good correlation (R2 = 0.9546) between the solidification threshold (low-frequency oscillation test determined by parallel-plate rheometer) and Tg of HME-filaments was also established.					
35131354	4	61	from	effect	585:590	arg1	printability					639:650	printability	639:650	printability	639:650	It explored and demonstrated the effect of HME-filament composition and fabrication on printability by evaluating thermal, mechanical, and thermo-rheological properties.					
35131354	5	62	theme	forms	828:832	arg1	manufacture					795:805	FDM-3DP manufacture	787:805	FDM-3DP manufacture of oral solid dosage forms	787:832	It also showed that the HME-Polymer filament composition used in FDM-3DP manufacture of oral solid dosage forms provides a tailored drug release profile.					
35131354	16	63	theme	internal	2572:2579	arg1	geometry					2581:2588	internal geometry	2572:2588	internal geometry (infill)	2572:2597	Drug-loaded and placebo HPMCAS-based formulations were shown to be successfully printed, with the former providing tailored drug release profiles based on variation of internal geometry (infill).					
35131354	16	63	theme	internal	2572:2579	arg1	infill					2591:2596	infill	2591:2596	infill	2591:2596	Drug-loaded and placebo HPMCAS-based formulations were shown to be successfully printed, with the former providing tailored drug release profiles based on variation of internal geometry (infill).					
35131354	4	64	theme	HME-filament	595:606	arg1	composition					608:618	HME-filament composition	595:618	HME-filament composition	595:618	It explored and demonstrated the effect of HME-filament composition and fabrication on printability by evaluating thermal, mechanical, and thermo-rheological properties.					
35131354	10	65	theme	nozzle	1584:1589	arg1	extrudate					1591:1599	the printer nozzle extrudate	1572:1599	the printer nozzle extrudate	1572:1599	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	12	66	theme	capillary	1912:1920	arg1	rheometer					1922:1930	a capillary rheometer	1910:1930	a capillary rheometer	1910:1930	The melt viscosity of HPMCAS formulations was investigated using a capillary rheometer.					
35131354	4	67	theme	mechanical	675:684	arg1	properties					710:719	thermal, mechanical, and thermo-rheological properties	666:719	thermal, mechanical, and thermo-rheological properties	666:719	It explored and demonstrated the effect of HME-filament composition and fabrication on printability by evaluating thermal, mechanical, and thermo-rheological properties.					
35131354	11	68	theme	lateral	1782:1788	arg1	wall					1790:1793	the lateral wall	1778:1793	the lateral wall of the printed tablet	1778:1815	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	7	69	theme	diverse	993:999	arg1	ways					1001:1004	Two diverse ways	989:1004	Two diverse ways of improving the mechanical properties of HME-filaments	989:1060	Two diverse ways of improving the mechanical properties of HME-filaments were deduced by changing the formulation to enable feeding through the roller gears of the printer nozzle.					
35131354	5	70	theme	release	859:865	arg1	profile					867:873	a tailored drug release profile	843:873	a tailored drug release profile	843:873	It also showed that the HME-Polymer filament composition used in FDM-3DP manufacture of oral solid dosage forms provides a tailored drug release profile.					
35131354	10	71	gly	inhomogeneity	1506:1518	arg1	HME-filament					1523:1534	HME-filament	1523:1534	HME-filament	1523:1534	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	11	72	theme	microscopy	1729:1738	arg1	images					1746:1751	scanning electron microscopy (SEM) images	1711:1751	scanning electron microscopy (SEM) images of nozzle extrudates	1711:1772	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	0	73	theme	fused	57:61	arg1	deposition					63:72	fused deposition	57:72	fused deposition	57:72	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.					
35131354	15	74	theme	parallel-plate	2332:2345	arg1	rheometer					2347:2355	parallel-plate rheometer	2332:2355	parallel-plate rheometer	2332:2355	A good correlation (R2 = 0.9546) between the solidification threshold (low-frequency oscillation test determined by parallel-plate rheometer) and Tg of HME-filaments was also established.					
35131354	9	75	theme	HME-filaments	1434:1446	arg1	values					1424:1429	glass-transition temperature (Tg) values	1390:1429	glass-transition temperature (Tg) values of HME-filaments	1390:1446	Experimental feedability was predicted using texture analysis results was a function of PEG concentration, and glass-transition temperature (Tg) values of HME-filaments.					
35131354	9	75	theme	HME-filaments	1434:1446	arg1	results					1341:1347	results	1341:1347	results	1341:1347	Experimental feedability was predicted using texture analysis results was a function of PEG concentration, and glass-transition temperature (Tg) values of HME-filaments.					
35131354	9	75	theme	HME-filaments	1434:1446	arg1	function					1355:1362	a function	1353:1362	a function of PEG concentration	1353:1383	Experimental feedability was predicted using texture analysis results was a function of PEG concentration, and glass-transition temperature (Tg) values of HME-filaments.					
35131354	1	76	theme	3D	133:134	arg1	printing					136:143	3D printing	133:143	3D printing (3DP) by fused deposition modelling (FDM)	133:185	3D printing (3DP) by fused deposition modelling (FDM) is one of the most extensively developed methods in additive manufacturing.					
35131354	1	76	theme	3D	133:134	arg1	3DP					146:148	3DP	146:148	3DP	146:148	3D printing (3DP) by fused deposition modelling (FDM) is one of the most extensively developed methods in additive manufacturing.					
35131354	11	77	theme	scanning	1711:1718	arg1	SEM					1741:1743	SEM	1741:1743	SEM	1741:1743	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	11	77	theme	scanning	1711:1718	arg1	microscopy					1729:1738	scanning electron microscopy	1711:1738	scanning electron microscopy (SEM) images of nozzle extrudates	1711:1772	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	12	78	theme	formulations	1874:1885	arg1	viscosity					1854:1862	The melt viscosity	1845:1862	The melt viscosity of HPMCAS formulations	1845:1885	The melt viscosity of HPMCAS formulations was investigated using a capillary rheometer.					
35131354	16	79	theme	geometry	2581:2588	arg1	variation					2559:2567	variation	2559:2567	variation of internal geometry (infill)	2559:2597	Drug-loaded and placebo HPMCAS-based formulations were shown to be successfully printed, with the former providing tailored drug release profiles based on variation of internal geometry (infill).					
35131354	14	80	dep	layers	2174:2179	arg1	using					2182:2186	using	2182:2186	using homogeneous HME-filaments	2182:2212	The plasticization of HPMCAS and the addition of talc into the formulation were shown to improve thickness consistency of printed layers (using homogeneous HME-filaments).					
35131354	14	81	theme	talc	2093:2096	arg1	HPMCAS					2066:2071	HPMCAS	2066:2071	HPMCAS	2066:2071	The plasticization of HPMCAS and the addition of talc into the formulation were shown to improve thickness consistency of printed layers (using homogeneous HME-filaments).					
35131354	14	81	theme	talc	2093:2096	arg1	addition					2081:2088	the addition	2077:2088	the addition of talc	2077:2096	The plasticization of HPMCAS and the addition of talc into the formulation were shown to improve thickness consistency of printed layers (using homogeneous HME-filaments).					
35131354	4	82	theme	thermal	666:672	arg1	properties					710:719	thermal, mechanical, and thermo-rheological properties	666:719	thermal, mechanical, and thermo-rheological properties	666:719	It explored and demonstrated the effect of HME-filament composition and fabrication on printability by evaluating thermal, mechanical, and thermo-rheological properties.					
35131354	2	83	theme	Optimizing	263:272	arg1	printability					274:285	Optimizing printability	263:285	Optimizing printability by improving feedability, nozzle extrusion, and layer deposition	263:350	Optimizing printability by improving feedability, nozzle extrusion, and layer deposition is crucial for manufacturing solid oral dosage forms with desirable properties.					
35131354	14	84	theme	addition	2081:2088	arg1	plasticization					2048:2061	The plasticization	2044:2061	The plasticization of HPMCAS and the addition of talc into the formulation	2044:2117	The plasticization of HPMCAS and the addition of talc into the formulation were shown to improve thickness consistency of printed layers (using homogeneous HME-filaments).					
35131354	0	85	theme	printability	94:105	arg1	enhancement					79:89	The enhancement	75:89	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.	0:131	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.					
35131354	9	86	theme	Experimental	1279:1290	arg1	feedability					1292:1302	Experimental feedability	1279:1302	Experimental feedability	1279:1302	Experimental feedability was predicted using texture analysis results was a function of PEG concentration, and glass-transition temperature (Tg) values of HME-filaments.					
35131354	12	87	theme	melt	1849:1852	arg1	viscosity					1854:1862	The melt viscosity	1845:1862	The melt viscosity of HPMCAS formulations	1845:1885	The melt viscosity of HPMCAS formulations was investigated using a capillary rheometer.					
35131354	7	88	theme	printer	1153:1159	arg1	nozzle					1161:1166	the printer nozzle	1149:1166	the printer nozzle	1149:1166	Two diverse ways of improving the mechanical properties of HME-filaments were deduced by changing the formulation to enable feeding through the roller gears of the printer nozzle.					
35131354	5	89	theme	FDM-3DP	787:793	arg1	manufacture					795:805	FDM-3DP manufacture	787:805	FDM-3DP manufacture of oral solid dosage forms	787:832	It also showed that the HME-Polymer filament composition used in FDM-3DP manufacture of oral solid dosage forms provides a tailored drug release profile.					
35131354	13	90	theme	nozzle	2026:2031	arg1	extrusion					2033:2041	nozzle extrusion	2026:2041	nozzle extrusion	2026:2041	The high viscosity of unplasticized HPMCAS was concluded to be an additional restriction for nozzle extrusion.					
35131354	10	91	theme	printed	1605:1611	arg1	layers					1613:1618	printed layers	1605:1618	printed layers	1605:1618	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	7	92	theme	roller	1133:1138	arg1	gears					1140:1144	the roller gears	1129:1144	the roller gears of the printer nozzle	1129:1166	Two diverse ways of improving the mechanical properties of HME-filaments were deduced by changing the formulation to enable feeding through the roller gears of the printer nozzle.					
35131354	1	93	theme	additive	239:246	arg1	manufacturing					248:260	additive manufacturing	239:260	additive manufacturing	239:260	3D printing (3DP) by fused deposition modelling (FDM) is one of the most extensively developed methods in additive manufacturing.					
35131354	16	94	theme	Drug-loaded	2404:2414	arg1	formulations					2441:2452	Drug-loaded and placebo HPMCAS-based formulations	2404:2452	Drug-loaded and placebo HPMCAS-based formulations	2404:2452	Drug-loaded and placebo HPMCAS-based formulations were shown to be successfully printed, with the former providing tailored drug release profiles based on variation of internal geometry (infill).					
35131354	10	95	theme	HME	1468:1470	arg1	100 rpm					1485:1491	100 rpm	1485:1491	100 rpm	1485:1491	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	10	95	theme	HME	1468:1470	arg1	speed					1478:1482	high HME screw speed	1463:1482	high HME screw speed (100 rpm)	1463:1492	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	2	96	theme	dosage	392:397	arg1	forms					399:403	solid oral dosage forms	381:403	solid oral dosage forms	381:403	Optimizing printability by improving feedability, nozzle extrusion, and layer deposition is crucial for manufacturing solid oral dosage forms with desirable properties.					
35131354	5	97	theme	filament	758:765	arg1	composition					767:777	the HME-Polymer filament composition	742:777	the HME-Polymer filament composition used in FDM-3DP manufacture of oral solid dosage forms	742:832	It also showed that the HME-Polymer filament composition used in FDM-3DP manufacture of oral solid dosage forms provides a tailored drug release profile.					
35131354	8	98	dep	include	1175:1181	arg1	plasticizing					1183:1194	plasticizing	1183:1194	plasticizing the polymer	1183:1206	These include plasticizing the polymer and adding an insoluble structuring agent (talc) into the formulation.					
35131354	8	98	dep	include	1175:1181	arg1	adding					1212:1217	adding	1212:1217	adding an insoluble structuring agent (talc) into the formulation	1212:1276	These include plasticizing the polymer and adding an insoluble structuring agent (talc) into the formulation.					
35131354	11	99	theme	temperature	1661:1671	arg1	variability					1625:1635	The variability	1621:1635	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet	1621:1815	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	15	100	theme	low-frequency	2287:2299	arg1	oscillation					2301:2311	low-frequency oscillation	2287:2311	low-frequency oscillation test	2287:2316	A good correlation (R2 = 0.9546) between the solidification threshold (low-frequency oscillation test determined by parallel-plate rheometer) and Tg of HME-filaments was also established.					
35131354	2	101	theme	solid	381:385	arg1	forms					399:403	solid oral dosage forms	381:403	solid oral dosage forms	381:403	Optimizing printability by improving feedability, nozzle extrusion, and layer deposition is crucial for manufacturing solid oral dosage forms with desirable properties.					
35131354	0	102	theme	forms	48:52	arg1	printing					3:10	3D printing	0:10	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.	0:131	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.					
35131354	11	103	theme	HME-filament	1685:1696	arg1	Tg					1674:1675	Tg	1674:1675	Tg	1674:1675	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	11	103	theme	HME-filament	1685:1696	arg1	temperature					1661:1671	the glass-transition temperature	1640:1671	the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates	1640:1772	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	11	103	theme	HME-filament	1685:1696	arg1	wall					1790:1793	the lateral wall	1778:1793	the lateral wall of the printed tablet	1778:1815	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	2	104	theme	nozzle	313:318	arg1	extrusion					320:328	nozzle extrusion	313:328	nozzle extrusion	313:328	Optimizing printability by improving feedability, nozzle extrusion, and layer deposition is crucial for manufacturing solid oral dosage forms with desirable properties.					
35131354	16	105	theme	tailored	2519:2526	arg1	profiles					2541:2548	tailored drug release profiles	2519:2548	tailored drug release profiles based on variation of internal geometry (infill)	2519:2597	Drug-loaded and placebo HPMCAS-based formulations were shown to be successfully printed, with the former providing tailored drug release profiles based on variation of internal geometry (infill).					
35131354	16	106	theme	HPMCAS-based	2428:2439	arg1	formulations					2441:2452	Drug-loaded and placebo HPMCAS-based formulations	2404:2452	Drug-loaded and placebo HPMCAS-based formulations	2404:2452	Drug-loaded and placebo HPMCAS-based formulations were shown to be successfully printed, with the former providing tailored drug release profiles based on variation of internal geometry (infill).					
35131354	10	107	theme	speed	1478:1482	arg1	effect					1453:1458	The effect	1449:1458	The effect of high HME screw speed (100 rpm)	1449:1492	The effect of high HME screw speed (100 rpm) resulted in inhomogeneity of HME-filament, which resulted in inconsistency of the printer nozzle extrudate and printed layers.					
35131354	11	108	theme	printed	1802:1808	arg1	tablet					1810:1815	the printed tablet	1798:1815	the printed tablet	1798:1815	The variability of the glass-transition temperature (Tg) of the HME-filament supported by scanning electron microscopy (SEM) images of nozzle extrudates and the lateral wall of the printed tablet helped explain this result.					
35131354	9	109	theme	PEG	1367:1369	arg1	concentration					1371:1383	PEG concentration	1367:1383	PEG concentration	1367:1383	Experimental feedability was predicted using texture analysis results was a function of PEG concentration, and glass-transition temperature (Tg) values of HME-filaments.					
35131354	16	110	theme	release	2533:2539	arg1	profiles					2541:2548	tailored drug release profiles	2519:2548	tailored drug release profiles based on variation of internal geometry (infill)	2519:2597	Drug-loaded and placebo HPMCAS-based formulations were shown to be successfully printed, with the former providing tailored drug release profiles based on variation of internal geometry (infill).					
35131354	0	111	dep	printing	3:10	arg1	enhancement					79:89	The enhancement	75:89	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.	0:131	3D printing of pharmaceutical oral solid dosage forms by fused deposition: The enhancement of printability using plasticised HPMCAS.					
35131354	13	112	theme	HPMCAS	1969:1974	arg1	restriction					2010:2020	an additional restriction	1996:2020	an additional restriction for nozzle extrusion	1996:2041	The high viscosity of unplasticized HPMCAS was concluded to be an additional restriction for nozzle extrusion.					
35131354	13	112	theme	HPMCAS	1969:1974	arg1	viscosity					1942:1950	The high viscosity	1933:1950	The high viscosity of unplasticized HPMCAS	1933:1974	The high viscosity of unplasticized HPMCAS was concluded to be an additional restriction for nozzle extrusion.					
35131354	2	113	theme	layer	335:339	arg1	deposition					341:350	layer deposition	335:350	layer deposition	335:350	Optimizing printability by improving feedability, nozzle extrusion, and layer deposition is crucial for manufacturing solid oral dosage forms with desirable properties.					
35131354	15	114	theme	oscillation	2301:2311	arg1	test					2313:2316	low-frequency oscillation test	2287:2316	low-frequency oscillation test	2287:2316	A good correlation (R2 = 0.9546) between the solidification threshold (low-frequency oscillation test determined by parallel-plate rheometer) and Tg of HME-filaments was also established.					
36604061	0	0	theme	ex	79:80	arg1	impregnation					87:98	its ex situ impregnation	75:98	its ex situ impregnation	75:98	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	6	1	theme	multiple	962:969	arg1	applications					971:982	multiple applications	962:982	multiple applications	962:982	The BC membrane was separately loaded with several fruit byproduct extracts to enhance its biological activity for multiple applications.					
36604061	8	2	theme	weight	1273:1278	arg1	loss					1280:1283	the fruits' weight loss	1261:1283	the fruits' weight loss of 15 %	1261:1291	The BC/PPE membrane enhanced the shelf-life storage of strawberry fruits by about 5 days, with a reduction in the fruits' weight loss of 15 % compared to the uncovered group.					
36604061	8	3	theme	BC/PPE	1155:1160	arg1	membrane					1162:1169	The BC/PPE membrane	1151:1169	The BC/PPE membrane	1151:1169	The BC/PPE membrane enhanced the shelf-life storage of strawberry fruits by about 5 days, with a reduction in the fruits' weight loss of 15 % compared to the uncovered group.					
36604061	6	4	theme	BC	851:852	arg1	membrane					854:861	The BC membrane	847:861	The BC membrane	847:861	The BC membrane was separately loaded with several fruit byproduct extracts to enhance its biological activity for multiple applications.					
36604061	6	5	theme	biological	938:947	arg1	activity					949:956	its biological activity	934:956	its biological activity for multiple applications	934:982	The BC membrane was separately loaded with several fruit byproduct extracts to enhance its biological activity for multiple applications.					
36604061	0	6	from	Optimization	0:11	arg1	impregnation					87:98	its ex situ impregnation	75:98	its ex situ impregnation	75:98	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	0	6	from	Optimization	0:11	arg1	peels					65:69	prickly pear peels	52:69	prickly pear peels	52:69	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	7	7	theme	antimicrobial	1070:1082	arg1	activity					1084:1091	significant broad-spectrum antimicrobial activity	1043:1091	significant broad-spectrum antimicrobial activity	1043:1091	BC loaded with pomegranate peel extract (BC/PPE) revealed significant broad-spectrum antimicrobial activity, followed by BC loaded with pomegranate molasses (BC/PM).					
36604061	8	8	from	reduction	1248:1256	arg1	loss					1280:1283	the fruits' weight loss	1261:1283	the fruits' weight loss of 15 %	1261:1291	The BC/PPE membrane enhanced the shelf-life storage of strawberry fruits by about 5 days, with a reduction in the fruits' weight loss of 15 % compared to the uncovered group.					
36604061	4	9	theme	maximum	587:593	arg1	production					598:607	the maximum BC production	583:607	the maximum BC production	583:607	The BC production was further optimized through a central composite design, where the maximum BC production was 6.01 g/L at 68 % PPPE at pH 4 after 11 days of incubation at 20 °C.					
36604061	4	10	from	11 days	649:655	arg1	20 °C					674:678	20 °C	674:678	20 °C	674:678	The BC production was further optimized through a central composite design, where the maximum BC production was 6.01 g/L at 68 % PPPE at pH 4 after 11 days of incubation at 20 °C.					
36604061	5	11	theme	promising	799:807	arg1	source					816:821	a promising carbon source	797:821	a promising carbon source for pure BC production	797:844	The produced BC was characterized by FT-IR spectroscopy, XRD, and SEM analysis, and the results showed that PPPE is a promising carbon source for pure BC production.					
36604061	5	11	theme	promising	799:807	arg1	PPPE					789:792	PPPE	789:792	PPPE	789:792	The produced BC was characterized by FT-IR spectroscopy, XRD, and SEM analysis, and the results showed that PPPE is a promising carbon source for pure BC production.					
36604061	0	12	theme	fruit	105:109	arg1	byproducts					111:120	fruit byproducts	105:120	fruit byproducts for antimicrobial and strawberry packaging applications	105:176	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	9	13	theme	strawberry	1460:1469	arg1	shelf-life					1471:1480	strawberry shelf-life	1460:1480	strawberry shelf-life when loaded with PPE or PM	1460:1507	The current study revealed the successful application of PPE for sustainable BC production with its packaging potential for enhancing strawberry shelf-life when loaded with PPE or PM.					
36604061	7	14	theme	significant	1043:1053	arg1	activity					1084:1091	significant broad-spectrum antimicrobial activity	1043:1091	significant broad-spectrum antimicrobial activity	1043:1091	BC loaded with pomegranate peel extract (BC/PPE) revealed significant broad-spectrum antimicrobial activity, followed by BC loaded with pomegranate molasses (BC/PM).					
36604061	9	15	theme	BC	1403:1404	arg1	production					1406:1415	sustainable BC production	1391:1415	sustainable BC production	1391:1415	The current study revealed the successful application of PPE for sustainable BC production with its packaging potential for enhancing strawberry shelf-life when loaded with PPE or PM.					
36604061	2	16	theme	production	273:282	arg1	costs					284:288	the production costs	269:288	the production costs	269:288	However, the production costs and biological inactivity are still challenges.					
36604061	2	16	theme	production	273:282	arg1	inactivity					305:314	biological inactivity	294:314	biological inactivity	294:314	However, the production costs and biological inactivity are still challenges.					
36604061	2	16	theme	production	273:282	arg1	challenges					326:335	challenges	326:335	challenges	326:335	However, the production costs and biological inactivity are still challenges.					
36604061	8	17	theme	fruits	1217:1222	arg1	storage					1195:1201	the shelf-life storage	1180:1201	the shelf-life storage of strawberry fruits	1180:1222	The BC/PPE membrane enhanced the shelf-life storage of strawberry fruits by about 5 days, with a reduction in the fruits' weight loss of 15 % compared to the uncovered group.					
36604061	1	18	theme	Bacterial	179:187	arg1	BC					200:201	BC	200:201	BC	200:201	Bacterial cellulose (BC) is currently among the most promising natural polymers.					
36604061	1	18	theme	Bacterial	179:187	arg1	cellulose					189:197	Bacterial cellulose	179:197	Bacterial cellulose (BC)	179:202	Bacterial cellulose (BC) is currently among the most promising natural polymers.					
36604061	5	19	theme	FT-IR	718:722	arg1	spectroscopy					724:735	FT-IR spectroscopy	718:735	FT-IR spectroscopy	718:735	The produced BC was characterized by FT-IR spectroscopy, XRD, and SEM analysis, and the results showed that PPPE is a promising carbon source for pure BC production.					
36604061	7	20	theme	broad-spectrum	1055:1068	arg1	activity					1084:1091	significant broad-spectrum antimicrobial activity	1043:1091	significant broad-spectrum antimicrobial activity	1043:1091	BC loaded with pomegranate peel extract (BC/PPE) revealed significant broad-spectrum antimicrobial activity, followed by BC loaded with pomegranate molasses (BC/PM).					
36604061	4	21	theme	BC	595:596	arg1	production					598:607	the maximum BC production	583:607	the maximum BC production	583:607	The BC production was further optimized through a central composite design, where the maximum BC production was 6.01 g/L at 68 % PPPE at pH 4 after 11 days of incubation at 20 °C.					
36604061	5	22	theme	carbon	809:814	arg1	source					816:821	a promising carbon source	797:821	a promising carbon source for pure BC production	797:844	The produced BC was characterized by FT-IR spectroscopy, XRD, and SEM analysis, and the results showed that PPPE is a promising carbon source for pure BC production.					
36604061	5	22	theme	carbon	809:814	arg1	PPPE					789:792	PPPE	789:792	PPPE	789:792	The produced BC was characterized by FT-IR spectroscopy, XRD, and SEM analysis, and the results showed that PPPE is a promising carbon source for pure BC production.					
36604061	3	23	theme	pear	403:406	arg1	PPP					415:417	PPP	415:417	PPP	415:417	The current study exploited the enzymatically hydrolyzed prickly pear peels (PPP) for BC production, which supported about 2.94 g/L as the sole production medium.					
36604061	3	23	theme	pear	403:406	arg1	peels					408:412	the enzymatically hydrolyzed prickly pear peels	366:412	the enzymatically hydrolyzed prickly pear peels (PPP)	366:418	The current study exploited the enzymatically hydrolyzed prickly pear peels (PPP) for BC production, which supported about 2.94 g/L as the sole production medium.					
36604061	6	24	theme	fruit	898:902	arg1	extracts					914:921	several fruit byproduct extracts	890:921	several fruit byproduct extracts	890:921	The BC membrane was separately loaded with several fruit byproduct extracts to enhance its biological activity for multiple applications.					
36604061	0	25	dep	ex	79:80	arg1	situ					82:85	situ	82:85	situ	82:85	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	7	26	theme	pomegranate	1000:1010	arg1	extract					1017:1023	pomegranate peel extract	1000:1023	pomegranate peel extract (BC/PPE)	1000:1032	BC loaded with pomegranate peel extract (BC/PPE) revealed significant broad-spectrum antimicrobial activity, followed by BC loaded with pomegranate molasses (BC/PM).					
36604061	7	26	theme	pomegranate	1000:1010	arg1	BC/PPE					1026:1031	BC/PPE	1026:1031	BC/PPE	1026:1031	BC loaded with pomegranate peel extract (BC/PPE) revealed significant broad-spectrum antimicrobial activity, followed by BC loaded with pomegranate molasses (BC/PM).					
36604061	3	27	theme	BC	424:425	arg1	production					427:436	BC production	424:436	BC production	424:436	The current study exploited the enzymatically hydrolyzed prickly pear peels (PPP) for BC production, which supported about 2.94 g/L as the sole production medium.					
36604061	7	28	theme	peel	1012:1015	arg1	extract					1017:1023	pomegranate peel extract	1000:1023	pomegranate peel extract (BC/PPE)	1000:1032	BC loaded with pomegranate peel extract (BC/PPE) revealed significant broad-spectrum antimicrobial activity, followed by BC loaded with pomegranate molasses (BC/PM).					
36604061	7	28	theme	peel	1012:1015	arg1	BC/PPE					1026:1031	BC/PPE	1026:1031	BC/PPE	1026:1031	BC loaded with pomegranate peel extract (BC/PPE) revealed significant broad-spectrum antimicrobial activity, followed by BC loaded with pomegranate molasses (BC/PM).					
36604061	4	29	theme	central	551:557	arg1	design					569:574	a central composite design	549:574	a central composite design	549:574	The BC production was further optimized through a central composite design, where the maximum BC production was 6.01 g/L at 68 % PPPE at pH 4 after 11 days of incubation at 20 °C.					
36604061	8	30	theme	strawberry	1206:1215	arg1	fruits					1217:1222	strawberry fruits	1206:1222	strawberry fruits	1206:1222	The BC/PPE membrane enhanced the shelf-life storage of strawberry fruits by about 5 days, with a reduction in the fruits' weight loss of 15 % compared to the uncovered group.					
36604061	0	31	theme	cellulose	26:34	arg1	production					36:45	bacterial cellulose production	16:45	bacterial cellulose production from prickly pear peels and its ex situ impregnation	16:98	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	8	32	theme	uncovered	1309:1317	arg1	group					1319:1323	the uncovered group	1305:1323	the uncovered group	1305:1323	The BC/PPE membrane enhanced the shelf-life storage of strawberry fruits by about 5 days, with a reduction in the fruits' weight loss of 15 % compared to the uncovered group.					
36604061	4	33	theme	68 	625:627	arg1	%					628:628	%	628:628	%	628:628	The BC production was further optimized through a central composite design, where the maximum BC production was 6.01 g/L at 68 % PPPE at pH 4 after 11 days of incubation at 20 °C.					
36604061	3	34	theme	prickly	395:401	arg1	PPP					415:417	PPP	415:417	PPP	415:417	The current study exploited the enzymatically hydrolyzed prickly pear peels (PPP) for BC production, which supported about 2.94 g/L as the sole production medium.					
36604061	3	34	theme	prickly	395:401	arg1	peels					408:412	the enzymatically hydrolyzed prickly pear peels	366:412	the enzymatically hydrolyzed prickly pear peels (PPP)	366:418	The current study exploited the enzymatically hydrolyzed prickly pear peels (PPP) for BC production, which supported about 2.94 g/L as the sole production medium.					
36604061	6	35	theme	byproduct	904:912	arg1	extracts					914:921	several fruit byproduct extracts	890:921	several fruit byproduct extracts	890:921	The BC membrane was separately loaded with several fruit byproduct extracts to enhance its biological activity for multiple applications.					
36604061	8	36	theme	%	1291:1291	arg1	loss					1280:1283	the fruits' weight loss	1261:1283	the fruits' weight loss of 15 %	1261:1291	The BC/PPE membrane enhanced the shelf-life storage of strawberry fruits by about 5 days, with a reduction in the fruits' weight loss of 15 % compared to the uncovered group.					
36604061	2	37	theme	biological	294:303	arg1	costs					284:288	the production costs	269:288	the production costs	269:288	However, the production costs and biological inactivity are still challenges.					
36604061	2	37	theme	biological	294:303	arg1	inactivity					305:314	biological inactivity	294:314	biological inactivity	294:314	However, the production costs and biological inactivity are still challenges.					
36604061	2	37	theme	biological	294:303	arg1	challenges					326:335	challenges	326:335	challenges	326:335	However, the production costs and biological inactivity are still challenges.					
36604061	9	38	theme	successful	1357:1366	arg1	application					1368:1378	the successful application	1353:1378	the successful application of PPE for sustainable BC production	1353:1415	The current study revealed the successful application of PPE for sustainable BC production with its packaging potential for enhancing strawberry shelf-life when loaded with PPE or PM.					
36604061	5	39	theme	pure	827:830	arg1	production					835:844	pure BC production	827:844	pure BC production	827:844	The produced BC was characterized by FT-IR spectroscopy, XRD, and SEM analysis, and the results showed that PPPE is a promising carbon source for pure BC production.					
36604061	4	40	theme	BC	505:506	arg1	production					508:517	The BC production	501:517	The BC production	501:517	The BC production was further optimized through a central composite design, where the maximum BC production was 6.01 g/L at 68 % PPPE at pH 4 after 11 days of incubation at 20 °C.					
36604061	4	41	theme	composite	559:567	arg1	design					569:574	a central composite design	549:574	a central composite design	549:574	The BC production was further optimized through a central composite design, where the maximum BC production was 6.01 g/L at 68 % PPPE at pH 4 after 11 days of incubation at 20 °C.					
36604061	5	42	theme	produced	685:692	arg1	BC					694:695	The produced BC	681:695	The produced BC	681:695	The produced BC was characterized by FT-IR spectroscopy, XRD, and SEM analysis, and the results showed that PPPE is a promising carbon source for pure BC production.					
36604061	9	43	theme	current	1330:1336	arg1	study					1338:1342	The current study	1326:1342	The current study	1326:1342	The current study revealed the successful application of PPE for sustainable BC production with its packaging potential for enhancing strawberry shelf-life when loaded with PPE or PM.					
36604061	0	44	theme	production	36:45	arg1	Optimization					0:11	Optimization	0:11	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.	0:177	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	6	45	theme	several	890:896	arg1	extracts					914:921	several fruit byproduct extracts	890:921	several fruit byproduct extracts	890:921	The BC membrane was separately loaded with several fruit byproduct extracts to enhance its biological activity for multiple applications.					
36604061	5	46	theme	SEM	747:749	arg1	analysis					751:758	SEM analysis	747:758	SEM analysis	747:758	The produced BC was characterized by FT-IR spectroscopy, XRD, and SEM analysis, and the results showed that PPPE is a promising carbon source for pure BC production.					
36604061	3	47	theme	hydrolyzed	384:393	arg1	PPP					415:417	PPP	415:417	PPP	415:417	The current study exploited the enzymatically hydrolyzed prickly pear peels (PPP) for BC production, which supported about 2.94 g/L as the sole production medium.					
36604061	3	47	theme	hydrolyzed	384:393	arg1	peels					408:412	the enzymatically hydrolyzed prickly pear peels	366:412	the enzymatically hydrolyzed prickly pear peels (PPP)	366:418	The current study exploited the enzymatically hydrolyzed prickly pear peels (PPP) for BC production, which supported about 2.94 g/L as the sole production medium.					
36604061	4	48	theme	%	628:628	arg1	PPPE					630:633	68 % PPPE	625:633	68 % PPPE	625:633	The BC production was further optimized through a central composite design, where the maximum BC production was 6.01 g/L at 68 % PPPE at pH 4 after 11 days of incubation at 20 °C.					
36604061	0	49	theme	pear	60:63	arg1	peels					65:69	prickly pear peels	52:69	prickly pear peels	52:69	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	0	50	theme	packaging	155:163	arg1	applications					165:176	strawberry packaging applications	144:176	strawberry packaging applications	144:176	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	0	51	from	peels	65:69	arg1	Optimization					0:11	Optimization	0:11	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.	0:177	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	0	51	from	peels	65:69	arg1	production					36:45	bacterial cellulose production	16:45	bacterial cellulose production from prickly pear peels and its ex situ impregnation	16:98	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	3	52	theme	production	482:491	arg1	medium					493:498	the sole production medium	473:498	the sole production medium	473:498	The current study exploited the enzymatically hydrolyzed prickly pear peels (PPP) for BC production, which supported about 2.94 g/L as the sole production medium.					
36604061	0	53	theme	prickly	52:58	arg1	peels					65:69	prickly pear peels	52:69	prickly pear peels	52:69	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	0	54	theme	strawberry	144:153	arg1	applications					165:176	strawberry packaging applications	144:176	strawberry packaging applications	144:176	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	9	55	theme	packaging	1426:1434	arg1	potential					1436:1444	its packaging potential	1422:1444	its packaging potential for enhancing strawberry shelf-life when loaded with PPE or PM	1422:1507	The current study revealed the successful application of PPE for sustainable BC production with its packaging potential for enhancing strawberry shelf-life when loaded with PPE or PM.					
36604061	7	56	theme	pomegranate	1121:1131	arg1	molasses					1133:1140	pomegranate molasses	1121:1140	pomegranate molasses (BC/PM)	1121:1148	BC loaded with pomegranate peel extract (BC/PPE) revealed significant broad-spectrum antimicrobial activity, followed by BC loaded with pomegranate molasses (BC/PM).					
36604061	7	56	theme	pomegranate	1121:1131	arg1	BC/PM					1143:1147	BC/PM	1143:1147	BC/PM	1143:1147	BC loaded with pomegranate peel extract (BC/PPE) revealed significant broad-spectrum antimicrobial activity, followed by BC loaded with pomegranate molasses (BC/PM).					
36604061	0	57	with	Optimization	0:11	arg1	byproducts					111:120	fruit byproducts	105:120	fruit byproducts for antimicrobial and strawberry packaging applications	105:176	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	0	58	from	impregnation	87:98	arg1	Optimization					0:11	Optimization	0:11	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.	0:177	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	0	58	from	impregnation	87:98	arg1	production					36:45	bacterial cellulose production	16:45	bacterial cellulose production from prickly pear peels and its ex situ impregnation	16:98	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	3	59	theme	sole	477:480	arg1	medium					493:498	the sole production medium	473:498	the sole production medium	473:498	The current study exploited the enzymatically hydrolyzed prickly pear peels (PPP) for BC production, which supported about 2.94 g/L as the sole production medium.					
36604061	8	60	theme	shelf-life	1184:1193	arg1	storage					1195:1201	the shelf-life storage	1180:1201	the shelf-life storage of strawberry fruits	1180:1222	The BC/PPE membrane enhanced the shelf-life storage of strawberry fruits by about 5 days, with a reduction in the fruits' weight loss of 15 % compared to the uncovered group.					
36604061	1	61	theme	most	227:230	arg1	polymers					250:257	the most promising natural polymers	223:257	the most promising natural polymers	223:257	Bacterial cellulose (BC) is currently among the most promising natural polymers.					
36604061	9	62	theme	sustainable	1391:1401	arg1	production					1406:1415	sustainable BC production	1391:1415	sustainable BC production	1391:1415	The current study revealed the successful application of PPE for sustainable BC production with its packaging potential for enhancing strawberry shelf-life when loaded with PPE or PM.					
36604061	4	63	theme	incubation	660:669	arg1	11 days					649:655	11 days	649:655	11 days of incubation at 20 °C	649:678	The BC production was further optimized through a central composite design, where the maximum BC production was 6.01 g/L at 68 % PPPE at pH 4 after 11 days of incubation at 20 °C.					
36604061	1	64	theme	promising	232:240	arg1	polymers					250:257	the most promising natural polymers	223:257	the most promising natural polymers	223:257	Bacterial cellulose (BC) is currently among the most promising natural polymers.					
36604061	3	65	theme	current	342:348	arg1	study					350:354	The current study	338:354	The current study	338:354	The current study exploited the enzymatically hydrolyzed prickly pear peels (PPP) for BC production, which supported about 2.94 g/L as the sole production medium.					
36604061	9	66	theme	PPE	1383:1385	arg1	application					1368:1378	the successful application	1353:1378	the successful application of PPE for sustainable BC production	1353:1415	The current study revealed the successful application of PPE for sustainable BC production with its packaging potential for enhancing strawberry shelf-life when loaded with PPE or PM.					
36604061	0	67	theme	bacterial	16:24	arg1	production					36:45	bacterial cellulose production	16:45	bacterial cellulose production from prickly pear peels and its ex situ impregnation	16:98	Optimization of bacterial cellulose production from prickly pear peels and its ex situ impregnation with fruit byproducts for antimicrobial and strawberry packaging applications.					
36604061	5	68	theme	BC	832:833	arg1	production					835:844	pure BC production	827:844	pure BC production	827:844	The produced BC was characterized by FT-IR spectroscopy, XRD, and SEM analysis, and the results showed that PPPE is a promising carbon source for pure BC production.					
36604061	4	69	from	20 °C	674:678	arg1	incubation					660:669	incubation	660:669	incubation at 20 °C	660:678	The BC production was further optimized through a central composite design, where the maximum BC production was 6.01 g/L at 68 % PPPE at pH 4 after 11 days of incubation at 20 °C.					
36604061	4	69	from	20 °C	674:678	arg1	11 days					649:655	11 days	649:655	11 days of incubation at 20 °C	649:678	The BC production was further optimized through a central composite design, where the maximum BC production was 6.01 g/L at 68 % PPPE at pH 4 after 11 days of incubation at 20 °C.					
36604061	1	70	theme	natural	242:248	arg1	polymers					250:257	the most promising natural polymers	223:257	the most promising natural polymers	223:257	Bacterial cellulose (BC) is currently among the most promising natural polymers.					
35930985	0	0	theme	rapid	95:99	arg1	hemostasis					101:110	rapid hemostasis	95:110	rapid hemostasis	95:110	Fabrication of microspheres containing coagulation factors by reverse microemulsion method for rapid hemostasis and wound healing.					
35930985	8	1	theme	closure	1160:1166	arg1	rate					1168:1171	the wound closure rate	1150:1171	the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days)	1150:1240	Additionally, the SP present in this modality also promoted fibroblast proliferation, this increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days).					
35930985	7	2	theme	bleeding	1032:1039	arg1	models					1041:1046	bleeding models	1032:1046	bleeding models	1032:1046	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	7	3	theme	@	913:913	arg1	Th					914:915	called CA/SP@Th	901:915	called CA/SP@Th	901:915	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	7	3	theme	@	913:913	arg1	platform					891:898	This platform	886:898	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can	886:990	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	2	4	theme	various	362:368	arg1	shapes					376:381	various wound shapes	362:381	various wound shapes	362:381	Microspheres that are biodegradable and adaptable to various wound shapes are a good alternative to traditional dressings.					
35930985	9	5	used	used	1270:1273	arg2	dressing					1296:1303	a multifunctional dressing	1278:1303	a multifunctional dressing for rapid hemostasis and wound healing	1278:1342	Therefore, CA/SP@Th can be used as a multifunctional dressing for rapid hemostasis and wound healing.					
35930985	9	5	used	used	1270:1273	arg2	Th					1260:1261	CA/SP@Th	1254:1261	CA/SP@Th	1254:1261	Therefore, CA/SP@Th can be used as a multifunctional dressing for rapid hemostasis and wound healing.					
35930985	7	6	theme	models	1041:1046	arg1	variety					1021:1027	a variety	1019:1027	a variety of bleeding models	1019:1046	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	7	6	theme	models	1041:1046	arg1	models					1041:1046	bleeding models	1032:1046	bleeding models	1032:1046	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	1	7	theme	wound	248:252	arg1	injury					254:259	wound injury	248:259	wound injury	248:259	Traditional dressings, such as bandages, gauze, and cotton pads stick to new granulation tissue, thereby aggravating wound injury or causing secondary injury during replacement.					
35930985	6	8	theme	loading	854:860	arg1	site					862:865	a stable loading site	845:865	a stable loading site for thrombin (Th)	845:883	In addition, the introducing SP provided a stable loading site for thrombin (Th).					
35930985	7	9	theme	called	901:906	arg1	Th					914:915	called CA/SP@Th	901:915	called CA/SP@Th	901:915	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	7	9	theme	called	901:906	arg1	platform					891:898	This platform	886:898	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can	886:990	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	5	10	theme	SP	703:704	arg1	content					706:712	the SP content	699:712	the SP content	699:712	By adjusting the SP content, the swelling rate of microspheres reached 1050 % and the pore diameter reached 19.59 nm.					
35930985	6	11	theme	stable	847:852	arg1	site					862:865	a stable loading site	845:865	a stable loading site for thrombin (Th)	845:883	In addition, the introducing SP provided a stable loading site for thrombin (Th).					
35930985	9	12	theme	multifunctional	1280:1294	arg1	dressing					1296:1303	a multifunctional dressing	1278:1303	a multifunctional dressing for rapid hemostasis and wound healing	1278:1342	Therefore, CA/SP@Th can be used as a multifunctional dressing for rapid hemostasis and wound healing.					
35930985	9	12	theme	multifunctional	1280:1294	arg1	Th					1260:1261	CA/SP@Th	1254:1261	CA/SP@Th	1254:1261	Therefore, CA/SP@Th can be used as a multifunctional dressing for rapid hemostasis and wound healing.					
35930985	8	13	theme	mouse	1198:1202	arg1	model					1204:1208	a total cortex injury mouse model	1176:1208	a total cortex injury mouse model	1176:1208	Additionally, the SP present in this modality also promoted fibroblast proliferation, this increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days).					
35930985	8	14	theme	injury	1191:1196	arg1	model					1204:1208	a total cortex injury mouse model	1176:1208	a total cortex injury mouse model	1176:1208	Additionally, the SP present in this modality also promoted fibroblast proliferation, this increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days).					
35930985	9	15	theme	rapid	1309:1313	arg1	hemostasis					1315:1324	rapid hemostasis	1309:1324	rapid hemostasis	1309:1324	Therefore, CA/SP@Th can be used as a multifunctional dressing for rapid hemostasis and wound healing.					
35930985	8	16	theme	wound	1154:1158	arg1	rate					1168:1171	the wound closure rate	1150:1171	the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days)	1150:1240	Additionally, the SP present in this modality also promoted fibroblast proliferation, this increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days).					
35930985	0	17	theme	wound	116:120	arg1	healing					122:128	wound healing	116:128	wound healing	116:128	Fabrication of microspheres containing coagulation factors by reverse microemulsion method for rapid hemostasis and wound healing.					
35930985	3	18	theme	silk	537:540	arg1	peptide					542:548	silk peptide	537:548	silk peptide (SP)	537:553	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	3	18	theme	silk	537:540	arg1	phase					570:574	the aqueous phase	558:574	the aqueous phase	558:574	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	3	18	theme	silk	537:540	arg1	SP					551:552	SP	551:552	SP	551:552	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	3	18	theme	silk	537:540	arg1	alginate					524:531	sodium alginate	517:531	sodium alginate	517:531	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	2	19	theme	good	389:392	arg1	alternative					394:404	a good alternative	387:404	a good alternative to traditional dressings	387:429	Microspheres that are biodegradable and adaptable to various wound shapes are a good alternative to traditional dressings.					
35930985	2	19	theme	good	389:392	arg1	Microspheres					309:320	Microspheres	309:320	Microspheres that are biodegradable and adaptable to various wound shapes	309:381	Microspheres that are biodegradable and adaptable to various wound shapes are a good alternative to traditional dressings.					
35930985	7	20	theme	rapid	999:1003	arg1	hemostasis					1005:1014	rapid hemostasis	999:1014	rapid hemostasis	999:1014	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	8	21	dep	rate	1168:1171	arg1	%					1224:1224	more than 97 %	1211:1224	more than 97 %	1211:1224	Additionally, the SP present in this modality also promoted fibroblast proliferation, this increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days).					
35930985	4	22	theme	composite	635:643	arg1	microspheres					645:656	calcium alginate (CA) and SP composite microspheres	606:656	microspheres	645:656	After cross-linking by Ca2+, calcium alginate (CA) and SP composite microspheres called CA/SP were prepared.					
35930985	1	23	theme	cotton	183:188	arg1	pads					190:193	cotton pads	183:193	cotton pads	183:193	Traditional dressings, such as bandages, gauze, and cotton pads stick to new granulation tissue, thereby aggravating wound injury or causing secondary injury during replacement.					
35930985	5	24	theme	pore	772:775	arg1	diameter					777:784	the pore diameter	768:784	the pore diameter	768:784	By adjusting the SP content, the swelling rate of microspheres reached 1050 % and the pore diameter reached 19.59 nm.					
35930985	1	25	theme	secondary	272:280	arg1	injury					282:287	secondary injury	272:287	secondary injury	272:287	Traditional dressings, such as bandages, gauze, and cotton pads stick to new granulation tissue, thereby aggravating wound injury or causing secondary injury during replacement.					
35930985	4	26	theme	alginate	614:621	arg1	CA					624:625	calcium alginate (CA) and SP composite microspheres	606:656	CA	624:625	After cross-linking by Ca2+, calcium alginate (CA) and SP composite microspheres called CA/SP were prepared.					
35930985	0	27	theme	microspheres	15:26	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of microspheres	0:26	Fabrication of microspheres containing coagulation factors by reverse microemulsion method for rapid hemostasis and wound healing.					
35930985	6	28	theme	introducing	821:831	arg1	SP					833:834	the introducing SP	817:834	the introducing SP	817:834	In addition, the introducing SP provided a stable loading site for thrombin (Th).					
35930985	4	29	theme	calcium	606:612	arg1	CA					624:625	calcium alginate (CA) and SP composite microspheres	606:656	CA	624:625	After cross-linking by Ca2+, calcium alginate (CA) and SP composite microspheres called CA/SP were prepared.					
35930985	5	30	theme	microspheres	736:747	arg1	rate					728:731	the swelling rate	715:731	the swelling rate of microspheres	715:747	By adjusting the SP content, the swelling rate of microspheres reached 1050 % and the pore diameter reached 19.59 nm.					
35930985	3	31	theme	microemulsion	490:502	arg1	method					504:509	reverse microemulsion method	482:509	reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase	482:574	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	8	32	theme	cortex	1184:1189	arg1	model					1204:1208	a total cortex injury mouse model	1176:1208	a total cortex injury mouse model	1176:1208	Additionally, the SP present in this modality also promoted fibroblast proliferation, this increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days).					
35930985	4	33	theme	SP	632:633	arg1	microspheres					645:656	calcium alginate (CA) and SP composite microspheres	606:656	microspheres	645:656	After cross-linking by Ca2+, calcium alginate (CA) and SP composite microspheres called CA/SP were prepared.					
35930985	2	34	theme	traditional	409:419	arg1	dressings					421:429	traditional dressings	409:429	traditional dressings	409:429	Microspheres that are biodegradable and adaptable to various wound shapes are a good alternative to traditional dressings.					
35930985	0	35	theme	coagulation	39:49	arg1	factors					51:57	coagulation factors	39:57	coagulation factors	39:57	Fabrication of microspheres containing coagulation factors by reverse microemulsion method for rapid hemostasis and wound healing.					
35930985	7	36	theme	Th	975:976	arg1	catalysis-can					978:990	Th catalysis-can	975:990	Th catalysis-can	975:990	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	8	37	theme	fibroblast	1109:1118	arg1	proliferation					1120:1132	fibroblast proliferation	1109:1132	fibroblast proliferation	1109:1132	Additionally, the SP present in this modality also promoted fibroblast proliferation, this increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days).					
35930985	7	38	theme	blood	936:940	arg1	enrichment					942:951	rapid blood enrichment	930:951	rapid blood enrichment	930:951	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	3	39	theme	novel	448:452	arg1	microsphere					454:464	a novel microsphere	446:464	a novel microsphere	446:464	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	0	40	theme	reverse	62:68	arg1	method					84:89	reverse microemulsion method	62:89	reverse microemulsion method for rapid hemostasis and wound healing	62:128	Fabrication of microspheres containing coagulation factors by reverse microemulsion method for rapid hemostasis and wound healing.					
35930985	5	41	theme	swelling	719:726	arg1	rate					728:731	the swelling rate	715:731	the swelling rate of microspheres	715:747	By adjusting the SP content, the swelling rate of microspheres reached 1050 % and the pore diameter reached 19.59 nm.					
35930985	3	42	theme	aqueous	562:568	arg1	peptide					542:548	silk peptide	537:548	silk peptide (SP)	537:553	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	3	42	theme	aqueous	562:568	arg1	phase					570:574	the aqueous phase	558:574	the aqueous phase	558:574	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	3	42	theme	aqueous	562:568	arg1	alginate					524:531	sodium alginate	517:531	sodium alginate	517:531	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	3	43	theme	reverse	482:488	arg1	method					504:509	reverse microemulsion method	482:509	reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase	482:574	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	8	44	dep	promoted	1100:1107	arg1	increased					1140:1148	increased	1140:1148	increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days)	1140:1240	Additionally, the SP present in this modality also promoted fibroblast proliferation, this increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days).					
35930985	9	45	theme	wound	1330:1334	arg1	healing					1336:1342	wound healing	1330:1342	wound healing	1330:1342	Therefore, CA/SP@Th can be used as a multifunctional dressing for rapid hemostasis and wound healing.					
35930985	1	46	theme	Traditional	131:141	arg1	dressings					143:151	Traditional dressings	131:151	Traditional dressings	131:151	Traditional dressings, such as bandages, gauze, and cotton pads stick to new granulation tissue, thereby aggravating wound injury or causing secondary injury during replacement.					
35930985	1	46	theme	Traditional	131:141	arg1	bandages					162:169	bandages	162:169	bandages	162:169	Traditional dressings, such as bandages, gauze, and cotton pads stick to new granulation tissue, thereby aggravating wound injury or causing secondary injury during replacement.					
35930985	9	47	theme	CA/SP	1254:1258	arg1	dressing					1296:1303	a multifunctional dressing	1278:1303	a multifunctional dressing for rapid hemostasis and wound healing	1278:1342	Therefore, CA/SP@Th can be used as a multifunctional dressing for rapid hemostasis and wound healing.					
35930985	9	47	theme	CA/SP	1254:1258	arg1	Th					1260:1261	CA/SP@Th	1254:1261	CA/SP@Th	1254:1261	Therefore, CA/SP@Th can be used as a multifunctional dressing for rapid hemostasis and wound healing.					
35930985	7	48	theme	rapid	930:934	arg1	enrichment					942:951	rapid blood enrichment	930:951	rapid blood enrichment	930:951	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	9	49	theme	@	1259:1259	arg1	dressing					1296:1303	a multifunctional dressing	1278:1303	a multifunctional dressing for rapid hemostasis and wound healing	1278:1342	Therefore, CA/SP@Th can be used as a multifunctional dressing for rapid hemostasis and wound healing.					
35930985	9	49	theme	@	1259:1259	arg1	Th					1260:1261	CA/SP@Th	1254:1261	CA/SP@Th	1254:1261	Therefore, CA/SP@Th can be used as a multifunctional dressing for rapid hemostasis and wound healing.					
35930985	1	50	theme	granulation	208:218	arg1	tissue					220:225	new granulation tissue	204:225	new granulation tissue	204:225	Traditional dressings, such as bandages, gauze, and cotton pads stick to new granulation tissue, thereby aggravating wound injury or causing secondary injury during replacement.					
35930985	7	51	theme	calcium	954:960	arg1	release					962:968	calcium release	954:968	calcium release	954:968	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	8	52	from	present	1070:1076	arg1	modality					1086:1093	this modality	1081:1093	this modality	1081:1093	Additionally, the SP present in this modality also promoted fibroblast proliferation, this increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days).					
35930985	8	53	from	rate	1168:1171	arg1	model					1204:1208	a total cortex injury mouse model	1176:1208	a total cortex injury mouse model	1176:1208	Additionally, the SP present in this modality also promoted fibroblast proliferation, this increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days).					
35930985	1	54	theme	new	204:206	arg1	tissue					220:225	new granulation tissue	204:225	new granulation tissue	204:225	Traditional dressings, such as bandages, gauze, and cotton pads stick to new granulation tissue, thereby aggravating wound injury or causing secondary injury during replacement.					
35930985	8	55	theme	total	1178:1182	arg1	model					1204:1208	a total cortex injury mouse model	1176:1208	a total cortex injury mouse model	1176:1208	Additionally, the SP present in this modality also promoted fibroblast proliferation, this increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days).					
35930985	3	56	theme	sodium	517:522	arg1	peptide					542:548	silk peptide	537:548	silk peptide (SP)	537:553	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	3	56	theme	sodium	517:522	arg1	phase					570:574	the aqueous phase	558:574	the aqueous phase	558:574	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	3	56	theme	sodium	517:522	arg1	alginate					524:531	sodium alginate	517:531	sodium alginate	517:531	In this work, a novel microsphere was prepared by reverse microemulsion method using sodium alginate and silk peptide (SP) as the aqueous phase.					
35930985	0	57	theme	microemulsion	70:82	arg1	method					84:89	reverse microemulsion method	62:89	reverse microemulsion method for rapid hemostasis and wound healing	62:128	Fabrication of microspheres containing coagulation factors by reverse microemulsion method for rapid hemostasis and wound healing.					
35930985	7	58	theme	CA/SP	908:912	arg1	Th					914:915	called CA/SP@Th	901:915	called CA/SP@Th	901:915	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	7	58	theme	CA/SP	908:912	arg1	platform					891:898	This platform	886:898	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can	886:990	This platform (called CA/SP@Th)-integrating rapid blood enrichment, calcium release, and Th catalysis-can ensure rapid hemostasis in a variety of bleeding models.					
35930985	2	59	theme	wound	370:374	arg1	shapes					376:381	various wound shapes	362:381	various wound shapes	362:381	Microspheres that are biodegradable and adaptable to various wound shapes are a good alternative to traditional dressings.					
35930985	8	60	theme	SP	1067:1068	arg1	present					1070:1076	the SP present	1063:1076	the SP present in this modality	1063:1093	Additionally, the SP present in this modality also promoted fibroblast proliferation, this increased the wound closure rate in a total cortex injury mouse model (more than 97 % within 15 days).					
36144543	4	0	theme	immobilized	793:803	arg1	bacteria					805:812	the immobilized bacteria	789:812	the immobilized bacteria	789:812	Based on the above exploration, the bacterial carrier was made, and the degradation efficiency of the immobilized bacteria reached 92.3% in 5 days.					
36144543	1	1	theme	removal	177:183	arg1	degradation					150:160	microbial degradation	140:160	microbial degradation	140:160	Estrogen contamination is widespread and microbial degradation is a promising removal method; however, unfavorable environments can hinder microbial function.					
36144543	1	1	theme	removal	177:183	arg1	method					185:190	a promising removal method	165:190	a promising removal method	165:190	Estrogen contamination is widespread and microbial degradation is a promising removal method; however, unfavorable environments can hinder microbial function.					
36144543	7	2	theme	5th	1205:1207	arg1	day					1209:1211	the 5th day	1201:1211	the 5th day	1201:1211	In the wastewater, the immobilized bacteria could degrade E2 to about 1 mg·L-1 on the 5th day.					
36144543	8	3	theme	new	1280:1282	arg1	combination					1284:1294	a new combination	1278:1294	a new combination	1278:1294	This study constructed a bacterial immobilization carrier using a new combination, explored the application potential of the carrier, and provided a new choice of bacterial immobilization carrier.					
36144543	6	4	theme	bacteria	1089:1096	arg1	efficiency					1059:1068	the degradation efficiency	1043:1068	the degradation efficiency of the immobilized bacteria	1043:1096	Immobilization showed advantages at pH 5, pH 11, 10 °C, 40 °C, and 40 g·L-1 NaCl, and the degradation efficiency of the immobilized bacteria was higher than 90%.					
36144543	6	4	theme	bacteria	1089:1096	arg1	higher					1102:1107	higher	1102:1107	higher	1102:1107	Immobilization showed advantages at pH 5, pH 11, 10 °C, 40 °C, and 40 g·L-1 NaCl, and the degradation efficiency of the immobilized bacteria was higher than 90%.					
36144543	0	5	from	Construction	0:11	arg1	Degradation					86:96	17β-Estradiol Degradation	72:96	17β-Estradiol Degradation	72:96	Construction of Magnetic Composite Bacterial Carrier and Application in 17β-Estradiol Degradation.					
36144543	4	6	theme	bacterial	727:735	arg1	carrier					737:743	the bacterial carrier	723:743	the bacterial carrier	723:743	Based on the above exploration, the bacterial carrier was made, and the degradation efficiency of the immobilized bacteria reached 92.3% in 5 days.					
36144543	3	7	theme	total	501:505	arg1	concentration					507:519	4% total concentration	498:519	4% total concentration	498:519	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	7	theme	total	501:505	arg1	5:5					531:533	PVA:SA = 5:5	522:533	PVA:SA = 5:5	522:533	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	0	8	from	Application	57:67	arg1	Degradation					86:96	17β-Estradiol Degradation	72:96	17β-Estradiol Degradation	72:96	Construction of Magnetic Composite Bacterial Carrier and Application in 17β-Estradiol Degradation.					
36144543	4	9	theme	degradation	763:773	arg1	efficiency					775:784	the degradation efficiency	759:784	the degradation efficiency of the immobilized bacteria	759:812	Based on the above exploration, the bacterial carrier was made, and the degradation efficiency of the immobilized bacteria reached 92.3% in 5 days.					
36144543	3	10	dep	concentration	507:519	arg1	maltose					563:569	maltose	563:569	maltose	563:569	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	10	dep	concentration	507:519	arg1	glycine					575:581	glycine	575:581	glycine	575:581	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	8	11	theme	application	1310:1320	arg1	potential					1322:1330	the application potential	1306:1330	the application potential of the carrier	1306:1345	This study constructed a bacterial immobilization carrier using a new combination, explored the application potential of the carrier, and provided a new choice of bacterial immobilization carrier.					
36144543	6	12	theme	immobilized	1077:1087	arg1	bacteria					1089:1096	the immobilized bacteria	1073:1096	the immobilized bacteria	1073:1096	Immobilization showed advantages at pH 5, pH 11, 10 °C, 40 °C, and 40 g·L-1 NaCl, and the degradation efficiency of the immobilized bacteria was higher than 90%.					
36144543	3	13	theme	4	498:498	arg1	%					499:499	%	499:499	%	499:499	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	7	14	theme	immobilized	1142:1152	arg1	bacteria					1154:1161	the immobilized bacteria	1138:1161	the immobilized bacteria	1138:1161	In the wastewater, the immobilized bacteria could degrade E2 to about 1 mg·L-1 on the 5th day.					
36144543	6	15	theme	g·L-1	1027:1031	arg1	NaCl					1033:1036	40 g·L-1 NaCl	1024:1036	40 g·L-1 NaCl	1024:1036	Immobilization showed advantages at pH 5, pH 11, 10 °C, 40 °C, and 40 g·L-1 NaCl, and the degradation efficiency of the immobilized bacteria was higher than 90%.					
36144543	2	16	theme	estrogen	283:290	arg1	target					343:348	a degradation target	329:348	a degradation target	329:348	In this study, a natural estrogen 17β-estradiol (E2) was introduced as a degradation target, and a new combination of bacterial carrier was investigated.					
36144543	2	16	theme	estrogen	283:290	arg1	E2					307:308	E2	307:308	E2	307:308	In this study, a natural estrogen 17β-estradiol (E2) was introduced as a degradation target, and a new combination of bacterial carrier was investigated.					
36144543	2	16	theme	estrogen	283:290	arg1	17β-estradiol					292:304	a natural estrogen 17β-estradiol	273:304	a natural estrogen 17β-estradiol (E2)	273:309	In this study, a natural estrogen 17β-estradiol (E2) was introduced as a degradation target, and a new combination of bacterial carrier was investigated.					
36144543	2	17	theme	carrier	386:392	arg1	combination					361:371	a new combination	355:371	a new combination of bacterial carrier	355:392	In this study, a natural estrogen 17β-estradiol (E2) was introduced as a degradation target, and a new combination of bacterial carrier was investigated.					
36144543	3	18	theme	optimal	627:633	arg1	concentrations					635:648	the optimal concentrations	623:648	the optimal concentrations	623:648	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	18	theme	optimal	627:633	arg1	g·L-1					657:661	5 g·L-1 and 10 g·L-1	655:674	g·L-1	657:661	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	1	19	theme	Estrogen	99:106	arg1	contamination					108:120	Estrogen contamination	99:120	Estrogen contamination	99:120	Estrogen contamination is widespread and microbial degradation is a promising removal method; however, unfavorable environments can hinder microbial function.					
36144543	2	20	theme	bacterial	376:384	arg1	carrier					386:392	bacterial carrier	376:392	bacterial carrier	376:392	In this study, a natural estrogen 17β-estradiol (E2) was introduced as a degradation target, and a new combination of bacterial carrier was investigated.					
36144543	3	21	dep	found	415:419	arg1	concentration					507:519	4% total concentration	498:519	4% total concentration	498:519	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	21	dep	found	415:419	arg1	5:5					531:533	PVA:SA = 5:5	522:533	PVA:SA = 5:5	522:533	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	22	theme	SA	526:527	arg1	concentration					507:519	4% total concentration	498:519	4% total concentration	498:519	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	22	theme	SA	526:527	arg1	5:5					531:533	PVA:SA = 5:5	522:533	PVA:SA = 5:5	522:533	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	1	23	theme	unfavorable	202:212	arg1	environments					214:225	unfavorable environments	202:225	unfavorable environments	202:225	Estrogen contamination is widespread and microbial degradation is a promising removal method; however, unfavorable environments can hinder microbial function.					
36144543	0	24	theme	Composite	25:33	arg1	Carrier					45:51	Magnetic Composite Bacterial Carrier	16:51	Magnetic Composite Bacterial Carrier	16:51	Construction of Magnetic Composite Bacterial Carrier and Application in 17β-Estradiol Degradation.					
36144543	6	25	theme	40	1024:1025	arg1	g·L-1					1027:1031	g·L-1	1027:1031	g·L-1	1027:1031	Immobilization showed advantages at pH 5, pH 11, 10 °C, 40 °C, and 40 g·L-1 NaCl, and the degradation efficiency of the immobilized bacteria was higher than 90%.					
36144543	8	26	theme	bacterial	1239:1247	arg1	carrier					1264:1270	a bacterial immobilization carrier	1237:1270	a bacterial immobilization carrier	1237:1270	This study constructed a bacterial immobilization carrier using a new combination, explored the application potential of the carrier, and provided a new choice of bacterial immobilization carrier.					
36144543	0	27	theme	Magnetic	16:23	arg1	Carrier					45:51	Magnetic Composite Bacterial Carrier	16:51	Magnetic Composite Bacterial Carrier	16:51	Construction of Magnetic Composite Bacterial Carrier and Application in 17β-Estradiol Degradation.					
36144543	3	28	theme	PVA	522:524	arg1	concentration					507:519	4% total concentration	498:519	4% total concentration	498:519	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	28	theme	PVA	522:524	arg1	5:5					531:533	PVA:SA = 5:5	522:533	PVA:SA = 5:5	522:533	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	5	29	theme	degradation	902:912	arg1	efficiency					914:923	the degradation efficiency	898:923	the degradation efficiency of each round	898:937	The immobilized bacteria were reused for three cycles, and the degradation efficiency of each round could exceed 94%.					
36144543	0	30	theme	Carrier	45:51	arg1	Construction					0:11	Construction	0:11	Construction of Magnetic Composite Bacterial Carrier	0:51	Construction of Magnetic Composite Bacterial Carrier and Application in 17β-Estradiol Degradation.					
36144543	0	30	theme	Carrier	45:51	arg1	Application					57:67	Application	57:67	Application in 17β-Estradiol Degradation	57:96	Construction of Magnetic Composite Bacterial Carrier and Application in 17β-Estradiol Degradation.					
36144543	3	31	theme	alginate	480:487	arg1	combination					430:440	the best combination	421:440	the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA)	421:492	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	6	32	theme	degradation	1047:1057	arg1	efficiency					1059:1068	the degradation efficiency	1043:1068	the degradation efficiency of the immobilized bacteria	1043:1096	Immobilization showed advantages at pH 5, pH 11, 10 °C, 40 °C, and 40 g·L-1 NaCl, and the degradation efficiency of the immobilized bacteria was higher than 90%.					
36144543	6	32	theme	degradation	1047:1057	arg1	higher					1102:1107	higher	1102:1107	higher	1102:1107	Immobilization showed advantages at pH 5, pH 11, 10 °C, 40 °C, and 40 g·L-1 NaCl, and the degradation efficiency of the immobilized bacteria was higher than 90%.					
36144543	3	33	theme	best	425:428	arg1	combination					430:440	the best combination	421:440	the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA)	421:492	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	34	theme	polyvinyl	445:453	arg1	alcohol					455:461	polyvinyl alcohol	445:461	polyvinyl alcohol (PVA)	445:467	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	34	theme	polyvinyl	445:453	arg1	PVA					464:466	PVA	464:466	PVA	464:466	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	0	35	theme	Bacterial	35:43	arg1	Carrier					45:51	Magnetic Composite Bacterial Carrier	16:51	Magnetic Composite Bacterial Carrier	16:51	Construction of Magnetic Composite Bacterial Carrier and Application in 17β-Estradiol Degradation.					
36144543	8	36	theme	new	1363:1365	arg1	choice					1367:1372	a new choice	1361:1372	a new choice of bacterial immobilization carrier	1361:1408	This study constructed a bacterial immobilization carrier using a new combination, explored the application potential of the carrier, and provided a new choice of bacterial immobilization carrier.					
36144543	8	37	theme	immobilization	1249:1262	arg1	carrier					1264:1270	a bacterial immobilization carrier	1237:1270	a bacterial immobilization carrier	1237:1270	This study constructed a bacterial immobilization carrier using a new combination, explored the application potential of the carrier, and provided a new choice of bacterial immobilization carrier.					
36144543	1	38	theme	microbial	238:246	arg1	function					248:255	microbial function	238:255	microbial function	238:255	Estrogen contamination is widespread and microbial degradation is a promising removal method; however, unfavorable environments can hinder microbial function.					
36144543	8	39	theme	carrier	1339:1345	arg1	potential					1322:1330	the application potential	1306:1330	the application potential of the carrier	1306:1345	This study constructed a bacterial immobilization carrier using a new combination, explored the application potential of the carrier, and provided a new choice of bacterial immobilization carrier.					
36144543	3	40	theme	=	529:529	arg1	concentration					507:519	4% total concentration	498:519	4% total concentration	498:519	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	40	theme	=	529:529	arg1	5:5					531:533	PVA:SA = 5:5	522:533	PVA:SA = 5:5	522:533	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	41	theme	sodium	473:478	arg1	SA					490:491	SA	490:491	SA	490:491	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	41	theme	sodium	473:478	arg1	alginate					480:487	sodium alginate	473:487	sodium alginate (SA)	473:492	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	42	with	concentration	507:519	arg1	%					556:556	nano-Fe3O4 at 2%	541:556	nano-Fe3O4 at 2%	541:556	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	1	43	theme	microbial	140:148	arg1	degradation					150:160	microbial degradation	140:160	microbial degradation	140:160	Estrogen contamination is widespread and microbial degradation is a promising removal method; however, unfavorable environments can hinder microbial function.					
36144543	1	43	theme	microbial	140:148	arg1	method					185:190	a promising removal method	165:190	a promising removal method	165:190	Estrogen contamination is widespread and microbial degradation is a promising removal method; however, unfavorable environments can hinder microbial function.					
36144543	4	44	theme	bacteria	805:812	arg1	efficiency					775:784	the degradation efficiency	759:784	the degradation efficiency of the immobilized bacteria	759:812	Based on the above exploration, the bacterial carrier was made, and the degradation efficiency of the immobilized bacteria reached 92.3% in 5 days.					
36144543	2	45	theme	natural	275:281	arg1	target					343:348	a degradation target	329:348	a degradation target	329:348	In this study, a natural estrogen 17β-estradiol (E2) was introduced as a degradation target, and a new combination of bacterial carrier was investigated.					
36144543	2	45	theme	natural	275:281	arg1	E2					307:308	E2	307:308	E2	307:308	In this study, a natural estrogen 17β-estradiol (E2) was introduced as a degradation target, and a new combination of bacterial carrier was investigated.					
36144543	2	45	theme	natural	275:281	arg1	17β-estradiol					292:304	a natural estrogen 17β-estradiol	273:304	a natural estrogen 17β-estradiol (E2)	273:309	In this study, a natural estrogen 17β-estradiol (E2) was introduced as a degradation target, and a new combination of bacterial carrier was investigated.					
36144543	2	46	theme	degradation	331:341	arg1	target					343:348	a degradation target	329:348	a degradation target	329:348	In this study, a natural estrogen 17β-estradiol (E2) was introduced as a degradation target, and a new combination of bacterial carrier was investigated.					
36144543	2	46	theme	degradation	331:341	arg1	17β-estradiol					292:304	a natural estrogen 17β-estradiol	273:304	a natural estrogen 17β-estradiol (E2)	273:309	In this study, a natural estrogen 17β-estradiol (E2) was introduced as a degradation target, and a new combination of bacterial carrier was investigated.					
36144543	8	47	theme	immobilization	1387:1400	arg1	carrier					1402:1408	bacterial immobilization carrier	1377:1408	bacterial immobilization carrier	1377:1408	This study constructed a bacterial immobilization carrier using a new combination, explored the application potential of the carrier, and provided a new choice of bacterial immobilization carrier.					
36144543	3	48	theme	%	499:499	arg1	concentration					507:519	4% total concentration	498:519	4% total concentration	498:519	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	3	48	theme	%	499:499	arg1	5:5					531:533	PVA:SA = 5:5	522:533	PVA:SA = 5:5	522:533	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	5	49	theme	round	933:937	arg1	efficiency					914:923	the degradation efficiency	898:923	the degradation efficiency of each round	898:937	The immobilized bacteria were reused for three cycles, and the degradation efficiency of each round could exceed 94%.					
36144543	5	50	theme	immobilized	843:853	arg1	bacteria					855:862	The immobilized bacteria	839:862	The immobilized bacteria	839:862	The immobilized bacteria were reused for three cycles, and the degradation efficiency of each round could exceed 94%.					
36144543	1	51	theme	promising	167:175	arg1	degradation					150:160	microbial degradation	140:160	microbial degradation	140:160	Estrogen contamination is widespread and microbial degradation is a promising removal method; however, unfavorable environments can hinder microbial function.					
36144543	1	51	theme	promising	167:175	arg1	method					185:190	a promising removal method	165:190	a promising removal method	165:190	Estrogen contamination is widespread and microbial degradation is a promising removal method; however, unfavorable environments can hinder microbial function.					
36144543	8	52	theme	bacterial	1377:1385	arg1	carrier					1402:1408	bacterial immobilization carrier	1377:1408	bacterial immobilization carrier	1377:1408	This study constructed a bacterial immobilization carrier using a new combination, explored the application potential of the carrier, and provided a new choice of bacterial immobilization carrier.					
36144543	0	53	theme	17β-Estradiol	72:84	arg1	Degradation					86:96	17β-Estradiol Degradation	72:96	17β-Estradiol Degradation	72:96	Construction of Magnetic Composite Bacterial Carrier and Application in 17β-Estradiol Degradation.					
36144543	3	54	theme	alcohol	455:461	arg1	combination					430:440	the best combination	421:440	the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA)	421:492	We found the best combination of polyvinyl alcohol (PVA) and sodium alginate (SA) was 4% total concentration, PVA:SA = 5:5, with nano-Fe3O4 at 2%, and maltose and glycine added to promote degradation, for which the optimal concentrations were 5 g·L-1 and 10 g·L-1, respectively.					
36144543	4	55	theme	above	704:708	arg1	exploration					710:720	the above exploration	700:720	the above exploration	700:720	Based on the above exploration, the bacterial carrier was made, and the degradation efficiency of the immobilized bacteria reached 92.3% in 5 days.					
36144543	2	56	theme	new	357:359	arg1	combination					361:371	a new combination	355:371	a new combination of bacterial carrier	355:392	In this study, a natural estrogen 17β-estradiol (E2) was introduced as a degradation target, and a new combination of bacterial carrier was investigated.					
36144543	8	57	theme	carrier	1402:1408	arg1	choice					1367:1372	a new choice	1361:1372	a new choice of bacterial immobilization carrier	1361:1408	This study constructed a bacterial immobilization carrier using a new combination, explored the application potential of the carrier, and provided a new choice of bacterial immobilization carrier.					
36436864	4	0	theme	sulfate	642:648	arg1	group					650:654	sulfate group	642:654	sulfate group	642:654	The chemical composition of CS before and after degradation were compared by FT-IR, NMR, etc., and no removement of sulfate group was observed.					
36436864	3	1	theme	molecular	480:488	arg1	weight					490:495	the average molecular weight	468:495	the average molecular weight of CS	468:501	In the present study, a photocatalytic degradation method using H2O2 and TiO2 has been developed and it could decrease the average molecular weight of CS into 5 kDa within 6 h.					
36436864	4	2	theme	CS	554:555	arg1	composition					539:549	The chemical composition	526:549	The chemical composition of CS before and after degradation	526:584	The chemical composition of CS before and after degradation were compared by FT-IR, NMR, etc., and no removement of sulfate group was observed.					
36436864	5	3	theme	reducing	853:860	arg1	ends					862:865	the reducing ends	849:865	the reducing ends	849:865	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy revealed that glucuronic acid or its derivative, arabinuronic acid, was at most of the reducing ends, and the depolymerization mechanism was proposed.					
36436864	5	4	from	product	737:743	arg1	identification					679:692	Then the identification	670:692	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy	670:764	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy revealed that glucuronic acid or its derivative, arabinuronic acid, was at most of the reducing ends, and the depolymerization mechanism was proposed.					
36436864	2	5	theme	degradation	282:292	arg1	methods					294:300	Effective degradation methods	272:300	Effective degradation methods	272:300	Effective degradation methods without striping sulfate groups are in a need.					
36436864	2	6	theme	Effective	272:280	arg1	methods					294:300	Effective degradation methods	272:300	Effective degradation methods	272:300	Effective degradation methods without striping sulfate groups are in a need.					
36436864	1	7	theme	molecular	254:262	arg1	weight					264:269	the molecular weight	250:269	the molecular weight	250:269	Chondroitin sulfate (CS) is widely known for its various biological activities which are closely related to the sulfate substitution and the molecular weight.					
36436864	0	8	theme	product	104:110	arg1	degradation					25:35	degradation	25:35	degradation of chondroitin sulfate and in vivo absorption	25:81	H2O2-TiO2 photocatalytic degradation of chondroitin sulfate and in vivo absorption and excertion of its product.					
36436864	0	8	theme	product	104:110	arg1	excertion					87:95	excertion	87:95	excertion of its product	87:110	H2O2-TiO2 photocatalytic degradation of chondroitin sulfate and in vivo absorption and excertion of its product.					
36436864	5	9	theme	degradation	725:735	arg1	product					737:743	the degradation product	721:743	the degradation product	721:743	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy revealed that glucuronic acid or its derivative, arabinuronic acid, was at most of the reducing ends, and the depolymerization mechanism was proposed.					
36436864	5	10	theme	arabinuronic	815:826	arg1	derivative					803:812	its derivative	799:812	its derivative	799:812	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy revealed that glucuronic acid or its derivative, arabinuronic acid, was at most of the reducing ends, and the depolymerization mechanism was proposed.					
36436864	5	10	theme	arabinuronic	815:826	arg1	acid					828:831	arabinuronic acid	815:831	arabinuronic acid	815:831	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy revealed that glucuronic acid or its derivative, arabinuronic acid, was at most of the reducing ends, and the depolymerization mechanism was proposed.					
36436864	4	11	theme	chemical	530:537	arg1	composition					539:549	The chemical composition	526:549	The chemical composition of CS before and after degradation	526:584	The chemical composition of CS before and after degradation were compared by FT-IR, NMR, etc., and no removement of sulfate group was observed.					
36436864	2	12	theme	sulfate	319:325	arg1	groups					327:332	sulfate groups	319:332	sulfate groups	319:332	Effective degradation methods without striping sulfate groups are in a need.					
36436864	1	13	theme	various	162:168	arg1	activities					181:190	its various biological activities	158:190	its various biological activities which are closely related to the sulfate substitution and the molecular weight	158:269	Chondroitin sulfate (CS) is widely known for its various biological activities which are closely related to the sulfate substitution and the molecular weight.					
36436864	6	14	from	absorption	934:943	arg1	rats					954:957	rats	954:957	rats	954:957	Furthermore, the absorption of CS in rats was enhanced by the degradation while the excertion profile of the degradation product was similar to that of CS.					
36436864	1	15	theme	biological	170:179	arg1	activities					181:190	its various biological activities	158:190	its various biological activities which are closely related to the sulfate substitution and the molecular weight	158:269	Chondroitin sulfate (CS) is widely known for its various biological activities which are closely related to the sulfate substitution and the molecular weight.					
36436864	3	16	theme	present	356:362	arg1	study					364:368	the present study	352:368	the present study	352:368	In the present study, a photocatalytic degradation method using H2O2 and TiO2 has been developed and it could decrease the average molecular weight of CS into 5 kDa within 6 h.					
36436864	5	17	theme	depolymerization	876:891	arg1	mechanism					893:901	the depolymerization mechanism	872:901	the depolymerization mechanism	872:901	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy revealed that glucuronic acid or its derivative, arabinuronic acid, was at most of the reducing ends, and the depolymerization mechanism was proposed.					
36436864	5	18	theme	glucuronic	780:789	arg1	acid					791:794	glucuronic acid	780:794	glucuronic acid	780:794	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy revealed that glucuronic acid or its derivative, arabinuronic acid, was at most of the reducing ends, and the depolymerization mechanism was proposed.					
36436864	5	19	from	identification	679:692	arg1	product					737:743	the degradation product	721:743	the degradation product	721:743	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy revealed that glucuronic acid or its derivative, arabinuronic acid, was at most of the reducing ends, and the depolymerization mechanism was proposed.					
36436864	0	20	theme	sulfate	52:58	arg1	degradation					25:35	degradation	25:35	degradation of chondroitin sulfate and in vivo absorption	25:81	H2O2-TiO2 photocatalytic degradation of chondroitin sulfate and in vivo absorption and excertion of its product.					
36436864	0	20	theme	sulfate	52:58	arg1	excertion					87:95	excertion	87:95	excertion of its product	87:110	H2O2-TiO2 photocatalytic degradation of chondroitin sulfate and in vivo absorption and excertion of its product.					
36436864	4	21	theme	group	650:654	arg1	removement					628:637	no removement	625:637	no removement of sulfate group	625:654	The chemical composition of CS before and after degradation were compared by FT-IR, NMR, etc., and no removement of sulfate group was observed.					
36436864	0	22	dep	in	64:65	arg1	vivo					67:70	vivo	67:70	vivo	67:70	H2O2-TiO2 photocatalytic degradation of chondroitin sulfate and in vivo absorption and excertion of its product.					
36436864	0	23	theme	chondroitin	40:50	arg1	sulfate					52:58	chondroitin sulfate	40:58	chondroitin sulfate	40:58	H2O2-TiO2 photocatalytic degradation of chondroitin sulfate and in vivo absorption and excertion of its product.					
36436864	3	24	theme	photocatalytic	373:386	arg1	method					400:405	a photocatalytic degradation method	371:405	a photocatalytic degradation method using H2O2 and TiO2	371:425	In the present study, a photocatalytic degradation method using H2O2 and TiO2 has been developed and it could decrease the average molecular weight of CS into 5 kDa within 6 h.					
36436864	5	25	theme	mass	748:751	arg1	spectroscopy					753:764	mass spectroscopy	748:764	mass spectroscopy	748:764	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy revealed that glucuronic acid or its derivative, arabinuronic acid, was at most of the reducing ends, and the depolymerization mechanism was proposed.					
36436864	5	26	theme	oligosaccharides	701:716	arg1	identification					679:692	Then the identification	670:692	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy	670:764	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy revealed that glucuronic acid or its derivative, arabinuronic acid, was at most of the reducing ends, and the depolymerization mechanism was proposed.					
36436864	1	27	theme	Chondroitin	113:123	arg1	CS					134:135	CS	134:135	CS	134:135	Chondroitin sulfate (CS) is widely known for its various biological activities which are closely related to the sulfate substitution and the molecular weight.					
36436864	1	27	theme	Chondroitin	113:123	arg1	sulfate					125:131	Chondroitin sulfate	113:131	Chondroitin sulfate (CS)	113:136	Chondroitin sulfate (CS) is widely known for its various biological activities which are closely related to the sulfate substitution and the molecular weight.					
36436864	3	28	theme	degradation	388:398	arg1	method					400:405	a photocatalytic degradation method	371:405	a photocatalytic degradation method using H2O2 and TiO2	371:425	In the present study, a photocatalytic degradation method using H2O2 and TiO2 has been developed and it could decrease the average molecular weight of CS into 5 kDa within 6 h.					
36436864	3	29	theme	average	472:478	arg1	weight					490:495	the average molecular weight	468:495	the average molecular weight of CS	468:501	In the present study, a photocatalytic degradation method using H2O2 and TiO2 has been developed and it could decrease the average molecular weight of CS into 5 kDa within 6 h.					
36436864	6	30	theme	degradation	1026:1036	arg1	product					1038:1044	the degradation product	1022:1044	the degradation product	1022:1044	Furthermore, the absorption of CS in rats was enhanced by the degradation while the excertion profile of the degradation product was similar to that of CS.					
36436864	0	31	theme	absorption	72:81	arg1	degradation					25:35	degradation	25:35	degradation of chondroitin sulfate and in vivo absorption	25:81	H2O2-TiO2 photocatalytic degradation of chondroitin sulfate and in vivo absorption and excertion of its product.					
36436864	0	31	theme	absorption	72:81	arg1	excertion					87:95	excertion	87:95	excertion of its product	87:110	H2O2-TiO2 photocatalytic degradation of chondroitin sulfate and in vivo absorption and excertion of its product.					
36436864	5	32	from	oligosaccharides	701:716	arg1	product					737:743	the degradation product	721:743	the degradation product	721:743	Then the identification of the oligosaccharides in the degradation product by mass spectroscopy revealed that glucuronic acid or its derivative, arabinuronic acid, was at most of the reducing ends, and the depolymerization mechanism was proposed.					
36436864	6	33	theme	product	1038:1044	arg1	similar					1050:1056	similar	1050:1056	similar	1050:1056	Furthermore, the absorption of CS in rats was enhanced by the degradation while the excertion profile of the degradation product was similar to that of CS.					
36436864	6	33	theme	product	1038:1044	arg1	profile					1011:1017	the excertion profile	997:1017	the excertion profile of the degradation product	997:1044	Furthermore, the absorption of CS in rats was enhanced by the degradation while the excertion profile of the degradation product was similar to that of CS.					
36436864	6	34	theme	excertion	1001:1009	arg1	similar					1050:1056	similar	1050:1056	similar	1050:1056	Furthermore, the absorption of CS in rats was enhanced by the degradation while the excertion profile of the degradation product was similar to that of CS.					
36436864	6	34	theme	excertion	1001:1009	arg1	profile					1011:1017	the excertion profile	997:1017	the excertion profile of the degradation product	997:1044	Furthermore, the absorption of CS in rats was enhanced by the degradation while the excertion profile of the degradation product was similar to that of CS.					
36436864	0	35	theme	in	64:65	arg1	absorption					72:81	in vivo absorption	64:81	in vivo absorption	64:81	H2O2-TiO2 photocatalytic degradation of chondroitin sulfate and in vivo absorption and excertion of its product.					
36436864	6	36	theme	CS	948:949	arg1	absorption					934:943	the absorption	930:943	the absorption of CS in rats	930:957	Furthermore, the absorption of CS in rats was enhanced by the degradation while the excertion profile of the degradation product was similar to that of CS.					
36436864	3	37	theme	CS	500:501	arg1	weight					490:495	the average molecular weight	468:495	the average molecular weight of CS	468:501	In the present study, a photocatalytic degradation method using H2O2 and TiO2 has been developed and it could decrease the average molecular weight of CS into 5 kDa within 6 h.					
36436864	1	38	theme	sulfate	225:231	arg1	substitution					233:244	the sulfate substitution	221:244	the sulfate substitution	221:244	Chondroitin sulfate (CS) is widely known for its various biological activities which are closely related to the sulfate substitution and the molecular weight.					
35251004	10	0	theme	potential	1693:1701	arg1	strategies					1714:1723	potential regulation strategies	1693:1723	potential regulation strategies	1693:1723	These findings provide potential regulation strategies and novel insights for RLS enhancing its protective effect in LPS-challenged broilers.					
35251004	3	1	theme	cecal	532:536	arg1	composition					548:558	the cecal microbial composition	528:558	the cecal microbial composition	528:558	16S rRNA gene sequencing and metabolomics were used for analyzing the cecal microbial composition and serum metabolites.					
35251004	9	2	theme	protective	1580:1589	arg1	effect					1591:1596	better protective effect	1573:1596	better protective effect	1573:1596	RLS exhibited better protective effect than antibiotic supplementation in the diet of LPS-challenged broilers.					
35251004	1	3	theme	following	167:175	arg1	characteristics					189:203	the following outstanding characteristics	163:203	the following outstanding characteristics	163:203	Rhamnolipid (RL) is a glycolipid biosurfactant and exhibits the following outstanding characteristics: strong antibacterial properties, low toxicity, and high biodegradability.					
35251004	7	4	theme	loss	1306:1309	arg1	rate					1311:1314	the decreased weight loss rate	1285:1314	the decreased weight loss rate	1285:1314	The genus Bacteroides may contribute to the decreased weight loss rate via regulating the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion.					
35251004	9	5	theme	broilers	1660:1667	arg1	diet					1637:1640	the diet	1633:1640	the diet of LPS-challenged broilers	1633:1667	RLS exhibited better protective effect than antibiotic supplementation in the diet of LPS-challenged broilers.					
35251004	4	6	theme	height	821:826	arg1	symptoms					778:785	the symptoms	774:785	the symptoms of intestinal bleeding and villus height	774:826	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	4	7	theme	diamine	711:717	arg1	oxidase					719:725	serum diamine oxidase	705:725	serum diamine oxidase	705:725	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	4	8	theme	loss	648:651	arg1	rate					653:656	the weight loss rate	637:656	the weight loss rate	637:656	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	8	9	theme	intestinal	1477:1486	arg1	microflora					1488:1497	intestinal microflora	1477:1497	intestinal microflora	1477:1497	RLS alleviates LPS-induced intestinal injury, enhances the growth and immunity, ameliorates intestinal microflora, and improves serum metabolites in LPS-challenged broilers.					
35251004	5	10	theme	LPS-challenged	1028:1041	arg1	broilers					1043:1050	LPS-challenged broilers	1028:1050	LPS-challenged broilers	1028:1050	The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group.					
35251004	7	11	theme	[20:5	1348:1352	arg1	secretion					1374:1382	the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion	1331:1382	the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion	1331:1382	The genus Bacteroides may contribute to the decreased weight loss rate via regulating the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion.					
35251004	4	12	theme	villus	814:819	arg1	height					821:826	villus height	814:826	villus height	814:826	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	8	13	from	metabolites	1519:1529	arg1	broilers					1549:1556	LPS-challenged broilers	1534:1556	LPS-challenged broilers	1534:1556	RLS alleviates LPS-induced intestinal injury, enhances the growth and immunity, ameliorates intestinal microflora, and improves serum metabolites in LPS-challenged broilers.					
35251004	4	14	theme	weight	641:646	arg1	loss					648:651	weight loss	641:651	the weight loss rate	637:656	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	7	15	theme	decreased	1289:1297	arg1	rate					1311:1314	the decreased weight loss rate	1285:1314	the decreased weight loss rate	1285:1314	The genus Bacteroides may contribute to the decreased weight loss rate via regulating the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion.					
35251004	5	16	theme	reduced	962:968	arg1	levels					1018:1023	reduced serum interleukin-6 and tumor necrosis factor-α levels	962:1023	reduced serum interleukin-6 and tumor necrosis factor-α levels	962:1023	The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group.					
35251004	5	17	theme	RLS	885:887	arg1	addition					873:880	The addition	869:880	The addition of RLS in the diet	869:899	The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group.					
35251004	4	18	theme	immunoglobulin	674:687	arg1	levels					689:694	serum immunoglobulin levels	668:694	serum immunoglobulin levels	668:694	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	3	19	theme	serum	564:568	arg1	metabolites					570:580	serum metabolites	564:580	serum metabolites	564:580	16S rRNA gene sequencing and metabolomics were used for analyzing the cecal microbial composition and serum metabolites.					
35251004	9	20	theme	better	1573:1578	arg1	effect					1591:1596	better protective effect	1573:1596	better protective effect	1573:1596	RLS exhibited better protective effect than antibiotic supplementation in the diet of LPS-challenged broilers.					
35251004	1	21	theme	outstanding	177:187	arg1	characteristics					189:203	the following outstanding characteristics	163:203	the following outstanding characteristics	163:203	Rhamnolipid (RL) is a glycolipid biosurfactant and exhibits the following outstanding characteristics: strong antibacterial properties, low toxicity, and high biodegradability.					
35251004	7	22	theme	weight	1299:1304	arg1	rate					1311:1314	the decreased weight loss rate	1285:1314	the decreased weight loss rate	1285:1314	The genus Bacteroides may contribute to the decreased weight loss rate via regulating the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion.					
35251004	10	23	theme	LPS-challenged	1787:1800	arg1	broilers					1802:1809	LPS-challenged broilers	1787:1809	LPS-challenged broilers	1787:1809	These findings provide potential regulation strategies and novel insights for RLS enhancing its protective effect in LPS-challenged broilers.					
35251004	3	24	theme	gene	471:474	arg1	sequencing					476:485	16S rRNA gene sequencing	462:485	16S rRNA gene sequencing	462:485	16S rRNA gene sequencing and metabolomics were used for analyzing the cecal microbial composition and serum metabolites.					
35251004	8	25	theme	intestinal	1412:1421	arg1	injury					1423:1428	LPS-induced intestinal injury	1400:1428	LPS-induced intestinal injury	1400:1428	RLS alleviates LPS-induced intestinal injury, enhances the growth and immunity, ameliorates intestinal microflora, and improves serum metabolites in LPS-challenged broilers.					
35251004	0	26	from	Effects	36:42	arg1	Broilers					93:100	Lipopolysaccharide Challenged Broilers	63:100	Lipopolysaccharide Challenged Broilers	63:100	Multi-Omics Revealed the Protective Effects of Rhamnolipids in Lipopolysaccharide Challenged Broilers.					
35251004	6	27	theme	inflammatory	1165:1176	arg1	responses					1178:1186	LPS-induced inflammatory responses	1153:1186	LPS-induced inflammatory responses	1153:1186	Spearman's correlation analysis revealed that RLS may alleviate LPS-induced inflammatory responses through altering the 6-methoxymellein level in broilers.					
35251004	10	28	theme	regulation	1703:1712	arg1	strategies					1714:1723	potential regulation strategies	1693:1723	potential regulation strategies	1693:1723	These findings provide potential regulation strategies and novel insights for RLS enhancing its protective effect in LPS-challenged broilers.					
35251004	5	29	theme	tumor	994:998	arg1	necrosis					1000:1007	tumor necrosis	994:1007	tumor necrosis	994:1007	The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group.					
35251004	5	30	from	addition	873:880	arg1	diet					896:899	the diet	892:899	the diet	892:899	The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group.					
35251004	5	31	theme	serum	910:914	arg1	interleukin-4					916:928	serum interleukin-4 and interleukin-10 contents	910:956	interleukin-4	916:928	The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group.					
35251004	10	32	theme	protective	1766:1775	arg1	effect					1777:1782	its protective effect	1762:1782	its protective effect	1762:1782	These findings provide potential regulation strategies and novel insights for RLS enhancing its protective effect in LPS-challenged broilers.					
35251004	2	33	theme	protective	330:339	arg1	alternative					386:396	an alternative	383:396	an alternative to antibiotics	383:411	The present research was conducted to explore the protective effects and mechanisms of rhamnolipids as an alternative to antibiotics in LPS (lipopolysaccharide)-challenged broilers.					
35251004	2	33	theme	protective	330:339	arg1	mechanisms					353:362	mechanisms	353:362	mechanisms	353:362	The present research was conducted to explore the protective effects and mechanisms of rhamnolipids as an alternative to antibiotics in LPS (lipopolysaccharide)-challenged broilers.					
35251004	2	33	theme	protective	330:339	arg1	effects					341:347	the protective effects	326:347	the protective effects	326:347	The present research was conducted to explore the protective effects and mechanisms of rhamnolipids as an alternative to antibiotics in LPS (lipopolysaccharide)-challenged broilers.					
35251004	1	34	theme	strong	206:211	arg1	properties					227:236	strong antibacterial properties	206:236	strong antibacterial properties	206:236	Rhamnolipid (RL) is a glycolipid biosurfactant and exhibits the following outstanding characteristics: strong antibacterial properties, low toxicity, and high biodegradability.					
35251004	0	35	theme	Protective	25:34	arg1	Effects					36:42	the Protective Effects	21:42	the Protective Effects of Rhamnolipids in Lipopolysaccharide Challenged Broilers	21:100	Multi-Omics Revealed the Protective Effects of Rhamnolipids in Lipopolysaccharide Challenged Broilers.					
35251004	4	36	theme	antibiotics	591:601	arg1	supplementation					611:625	Dietary antibiotics and RLS supplementation	583:625	Dietary antibiotics and RLS supplementation	583:625	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	8	37	theme	LPS-induced	1400:1410	arg1	injury					1423:1428	LPS-induced intestinal injury	1400:1428	LPS-induced intestinal injury	1400:1428	RLS alleviates LPS-induced intestinal injury, enhances the growth and immunity, ameliorates intestinal microflora, and improves serum metabolites in LPS-challenged broilers.					
35251004	5	38	theme	factor-α	1009:1016	arg1	levels					1018:1023	reduced serum interleukin-6 and tumor necrosis factor-α levels	962:1023	reduced serum interleukin-6 and tumor necrosis factor-α levels	962:1023	The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group.					
35251004	6	39	theme	LPS-induced	1153:1163	arg1	responses					1178:1186	LPS-induced inflammatory responses	1153:1186	LPS-induced inflammatory responses	1153:1186	Spearman's correlation analysis revealed that RLS may alleviate LPS-induced inflammatory responses through altering the 6-methoxymellein level in broilers.					
35251004	1	40	theme	antibacterial	213:225	arg1	properties					227:236	strong antibacterial properties	206:236	strong antibacterial properties	206:236	Rhamnolipid (RL) is a glycolipid biosurfactant and exhibits the following outstanding characteristics: strong antibacterial properties, low toxicity, and high biodegradability.					
35251004	5	41	theme	necrosis	1000:1007	arg1	levels					1018:1023	reduced serum interleukin-6 and tumor necrosis factor-α levels	962:1023	reduced serum interleukin-6 and tumor necrosis factor-α levels	962:1023	The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group.					
35251004	7	42	dep	secretion	1374:1382	arg1	5Z,8Z,11Z,14Z,17Z					1354:1370	5Z,8Z,11Z,14Z,17Z	1354:1370	5Z,8Z,11Z,14Z,17Z	1354:1370	The genus Bacteroides may contribute to the decreased weight loss rate via regulating the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion.					
35251004	3	43	theme	microbial	538:546	arg1	composition					548:558	the cecal microbial composition	528:558	the cecal microbial composition	528:558	16S rRNA gene sequencing and metabolomics were used for analyzing the cecal microbial composition and serum metabolites.					
35251004	2	44	theme	-challenged	440:450	arg1	broilers					452:459	LPS (lipopolysaccharide)-challenged broilers	416:459	LPS (lipopolysaccharide)-challenged broilers	416:459	The present research was conducted to explore the protective effects and mechanisms of rhamnolipids as an alternative to antibiotics in LPS (lipopolysaccharide)-challenged broilers.					
35251004	7	45	theme	genus	1249:1253	arg1	Bacteroides					1255:1265	The genus Bacteroides	1245:1265	The genus Bacteroides	1245:1265	The genus Bacteroides may contribute to the decreased weight loss rate via regulating the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion.					
35251004	4	46	theme	RLS	607:609	arg1	supplementation					611:625	Dietary antibiotics and RLS supplementation	583:625	Dietary antibiotics and RLS supplementation	583:625	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	3	47	theme	16S	462:464	arg1	sequencing					476:485	16S rRNA gene sequencing	462:485	16S rRNA gene sequencing	462:485	16S rRNA gene sequencing and metabolomics were used for analyzing the cecal microbial composition and serum metabolites.					
35251004	9	48	theme	antibiotic	1603:1612	arg1	supplementation					1614:1628	antibiotic supplementation	1603:1628	antibiotic supplementation in the diet of LPS-challenged broilers	1603:1667	RLS exhibited better protective effect than antibiotic supplementation in the diet of LPS-challenged broilers.					
35251004	3	49	theme	rRNA	466:469	arg1	sequencing					476:485	16S rRNA gene sequencing	462:485	16S rRNA gene sequencing	462:485	16S rRNA gene sequencing and metabolomics were used for analyzing the cecal microbial composition and serum metabolites.					
35251004	10	50	theme	novel	1729:1733	arg1	insights					1735:1742	novel insights	1729:1742	novel insights	1729:1742	These findings provide potential regulation strategies and novel insights for RLS enhancing its protective effect in LPS-challenged broilers.					
35251004	3	51	used	used	509:512	arg2	metabolomics					491:502	metabolomics	491:502	metabolomics	491:502	16S rRNA gene sequencing and metabolomics were used for analyzing the cecal microbial composition and serum metabolites.					
35251004	3	51	used	used	509:512	arg2	sequencing					476:485	16S rRNA gene sequencing	462:485	16S rRNA gene sequencing	462:485	16S rRNA gene sequencing and metabolomics were used for analyzing the cecal microbial composition and serum metabolites.					
35251004	6	52	from	level	1226:1230	arg1	broilers					1235:1242	broilers	1235:1242	broilers	1235:1242	Spearman's correlation analysis revealed that RLS may alleviate LPS-induced inflammatory responses through altering the 6-methoxymellein level in broilers.					
35251004	1	53	theme	low	239:241	arg1	toxicity					243:250	low toxicity	239:250	low toxicity	239:250	Rhamnolipid (RL) is a glycolipid biosurfactant and exhibits the following outstanding characteristics: strong antibacterial properties, low toxicity, and high biodegradability.					
35251004	7	54	theme	serum	1335:1339	arg1	lysoPC					1341:1346	the serum lysoPC	1331:1346	the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion	1331:1382	The genus Bacteroides may contribute to the decreased weight loss rate via regulating the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion.					
35251004	9	55	from	supplementation	1614:1628	arg1	diet					1637:1640	the diet	1633:1640	the diet of LPS-challenged broilers	1633:1667	RLS exhibited better protective effect than antibiotic supplementation in the diet of LPS-challenged broilers.					
35251004	2	56	theme	rhamnolipids	367:378	arg1	alternative					386:396	an alternative	383:396	an alternative to antibiotics	383:411	The present research was conducted to explore the protective effects and mechanisms of rhamnolipids as an alternative to antibiotics in LPS (lipopolysaccharide)-challenged broilers.					
35251004	2	56	theme	rhamnolipids	367:378	arg1	mechanisms					353:362	mechanisms	353:362	mechanisms	353:362	The present research was conducted to explore the protective effects and mechanisms of rhamnolipids as an alternative to antibiotics in LPS (lipopolysaccharide)-challenged broilers.					
35251004	2	56	theme	rhamnolipids	367:378	arg1	effects					341:347	the protective effects	326:347	the protective effects	326:347	The present research was conducted to explore the protective effects and mechanisms of rhamnolipids as an alternative to antibiotics in LPS (lipopolysaccharide)-challenged broilers.					
35251004	0	57	theme	Rhamnolipids	47:58	arg1	Effects					36:42	the Protective Effects	21:42	the Protective Effects of Rhamnolipids in Lipopolysaccharide Challenged Broilers	21:100	Multi-Omics Revealed the Protective Effects of Rhamnolipids in Lipopolysaccharide Challenged Broilers.					
35251004	4	58	theme	Dietary	583:589	arg1	supplementation					611:625	Dietary antibiotics and RLS supplementation	583:625	Dietary antibiotics and RLS supplementation	583:625	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	8	59	theme	serum	1513:1517	arg1	metabolites					1519:1529	serum metabolites	1513:1529	serum metabolites in LPS-challenged broilers	1513:1556	RLS alleviates LPS-induced intestinal injury, enhances the growth and immunity, ameliorates intestinal microflora, and improves serum metabolites in LPS-challenged broilers.					
35251004	5	60	theme	serum	970:974	arg1	interleukin-6					976:988	serum interleukin-6	970:988	serum interleukin-6	970:988	The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group.					
35251004	8	61	theme	LPS-challenged	1534:1547	arg1	broilers					1549:1556	LPS-challenged broilers	1534:1556	LPS-challenged broilers	1534:1556	RLS alleviates LPS-induced intestinal injury, enhances the growth and immunity, ameliorates intestinal microflora, and improves serum metabolites in LPS-challenged broilers.					
35251004	2	62	theme	present	284:290	arg1	research					292:299	The present research	280:299	The present research	280:299	The present research was conducted to explore the protective effects and mechanisms of rhamnolipids as an alternative to antibiotics in LPS (lipopolysaccharide)-challenged broilers.					
35251004	1	63	theme	glycolipid	125:134	arg1	biosurfactant					136:148	a glycolipid biosurfactant	123:148	a glycolipid biosurfactant	123:148	Rhamnolipid (RL) is a glycolipid biosurfactant and exhibits the following outstanding characteristics: strong antibacterial properties, low toxicity, and high biodegradability.					
35251004	1	63	theme	glycolipid	125:134	arg1	Rhamnolipid					103:113	Rhamnolipid	103:113	Rhamnolipid (RL)	103:118	Rhamnolipid (RL) is a glycolipid biosurfactant and exhibits the following outstanding characteristics: strong antibacterial properties, low toxicity, and high biodegradability.					
35251004	6	64	theme	6-methoxymellein	1209:1224	arg1	level					1226:1230	the 6-methoxymellein level	1205:1230	the 6-methoxymellein level in broilers	1205:1242	Spearman's correlation analysis revealed that RLS may alleviate LPS-induced inflammatory responses through altering the 6-methoxymellein level in broilers.					
35251004	6	65	theme	correlation	1100:1110	arg1	analysis					1112:1119	Spearman's correlation analysis	1089:1119	Spearman's correlation analysis	1089:1119	Spearman's correlation analysis revealed that RLS may alleviate LPS-induced inflammatory responses through altering the 6-methoxymellein level in broilers.					
35251004	4	66	theme	bleeding	801:808	arg1	symptoms					778:785	the symptoms	774:785	the symptoms of intestinal bleeding and villus height	774:826	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	5	67	theme	interleukin-10	934:947	arg1	contents					949:956	serum interleukin-4 and interleukin-10 contents	910:956	contents	949:956	The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group.					
35251004	5	68	theme	interleukin-6	976:988	arg1	levels					1018:1023	reduced serum interleukin-6 and tumor necrosis factor-α levels	962:1023	reduced serum interleukin-6 and tumor necrosis factor-α levels	962:1023	The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group.					
35251004	0	69	theme	Challenged	82:91	arg1	Broilers					93:100	Lipopolysaccharide Challenged Broilers	63:100	Lipopolysaccharide Challenged Broilers	63:100	Multi-Omics Revealed the Protective Effects of Rhamnolipids in Lipopolysaccharide Challenged Broilers.					
35251004	5	70	theme	antibiotics	1070:1080	arg1	group					1082:1086	the antibiotics group	1066:1086	the antibiotics group	1066:1086	The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group.					
35251004	9	71	theme	LPS-challenged	1645:1658	arg1	broilers					1660:1667	LPS-challenged broilers	1645:1667	LPS-challenged broilers	1645:1667	RLS exhibited better protective effect than antibiotic supplementation in the diet of LPS-challenged broilers.					
35251004	4	72	theme	acid	741:744	arg1	concentration					746:758	D-lactate acid concentration	731:758	D-lactate acid concentration	731:758	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	4	73	theme	serum	705:709	arg1	oxidase					719:725	serum diamine oxidase	705:725	serum diamine oxidase	705:725	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	4	74	theme	serum	668:672	arg1	levels					689:694	serum immunoglobulin levels	668:694	serum immunoglobulin levels	668:694	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	0	75	theme	Lipopolysaccharide	63:80	arg1	Broilers					93:100	Lipopolysaccharide Challenged Broilers	63:100	Lipopolysaccharide Challenged Broilers	63:100	Multi-Omics Revealed the Protective Effects of Rhamnolipids in Lipopolysaccharide Challenged Broilers.					
35251004	4	76	theme	D-lactate	731:739	arg1	concentration					746:758	D-lactate acid concentration	731:758	D-lactate acid concentration	731:758	Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS.					
35251004	7	77	theme	lysoPC	1341:1346	arg1	secretion					1374:1382	the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion	1331:1382	the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion	1331:1382	The genus Bacteroides may contribute to the decreased weight loss rate via regulating the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion.					
35251004	1	78	theme	high	257:260	arg1	biodegradability					262:277	high biodegradability	257:277	high biodegradability	257:277	Rhamnolipid (RL) is a glycolipid biosurfactant and exhibits the following outstanding characteristics: strong antibacterial properties, low toxicity, and high biodegradability.					
36870661	7	0	theme	films	1485:1489	arg1	biodegradability					1451:1466	the excellent biodegradability	1437:1466	the excellent biodegradability of the packaging films for environmental protection	1437:1518	Furthermore, the biodegradation rate of food packaging films cultured with natural soil was 95 % after 8 days, clarifying the excellent biodegradability of the packaging films for environmental protection.					
36870661	1	1	theme	alternative	330:340	arg1	microcapsules					301:313	polyurethane-encapsulated essential-oil microcapsules	261:313	polyurethane-encapsulated essential-oil microcapsules (EOs@PU)	261:322	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	1	1	theme	alternative	330:340	arg1	preservative					352:363	an alternative synthetic preservative	327:363	an alternative synthetic preservative for food preservation	327:385	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	4	2	theme	packaging	907:915	arg1	films					917:921	food packaging films	902:921	food packaging films	902:921	As such, we further integrated the obtained EOs@PU microcapsules into potato starch to prepare food packaging films for sustained food preservation.					
36870661	3	3	theme	loading	779:785	arg1	%					803:803	59.01 %	797:803	59.01 %	797:803	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	3	3	theme	loading	779:785	arg1	capacity					787:794	high loading capacity	774:794	high loading capacity (59.01 %)	774:804	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	6	4	theme	fresh	1260:1264	arg1	blueberries					1266:1276	fresh blueberries	1260:1276	fresh blueberries	1260:1276	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	6	5	theme	microcapsules	1157:1169	arg1	release					1139:1145	the long-term release	1125:1145	the long-term release of EOs@PU microcapsules	1125:1169	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	3	6	with	regular	696:702	arg1	size					732:735	an average size	721:735	an average size of approximately 3 μm	721:757	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	1	7	theme	polyurethane-encapsulated	261:285	arg1	microcapsules					301:313	polyurethane-encapsulated essential-oil microcapsules	261:313	polyurethane-encapsulated essential-oil microcapsules (EOs@PU)	261:322	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	1	7	theme	polyurethane-encapsulated	261:285	arg1	PU					320:321	EOs@PU	316:321	EOs@PU	316:321	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	1	7	theme	polyurethane-encapsulated	261:285	arg1	preservative					352:363	an alternative synthetic preservative	327:363	an alternative synthetic preservative for food preservation	327:385	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	2	8	theme	interfacial	609:619	arg1	polymerization					621:634	interfacial polymerization	609:634	interfacial polymerization	609:634	Herein, three essential oils (EOs) were blended to make composite essential oils with a more harmonious aroma and higher antibacterial ability and encapsulated into polyurethane (PU) to form EOs@PU microcapsules based on interfacial polymerization.					
36870661	6	9	theme	@	1153:1153	arg1	microcapsules					1157:1169	EOs@PU microcapsules	1150:1169	EOs@PU microcapsules	1150:1169	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	6	10	theme	shelf	1246:1250	arg1	life					1252:1255	the shelf life	1242:1255	the shelf life of fresh blueberries and raspberries	1242:1292	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	5	11	theme	low	1097:1099	arg1	toxicity					1106:1113	low cell toxicity	1097:1113	low cell toxicity	1097:1113	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	3	12	theme	EOs	671:673	arg1	microcapsules					678:690	the constructed EOs@PU microcapsules	655:690	the constructed EOs@PU microcapsules	655:690	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	1	13	theme	EOs	316:318	arg1	microcapsules					301:313	polyurethane-encapsulated essential-oil microcapsules	261:313	polyurethane-encapsulated essential-oil microcapsules (EOs@PU)	261:322	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	1	13	theme	EOs	316:318	arg1	PU					320:321	EOs@PU	316:321	EOs@PU	316:321	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	8	14	theme	biodegradable	1542:1554	arg1	films					1566:1570	the biodegradable packaging films	1538:1570	the biodegradable packaging films	1538:1570	As demonstrated, the biodegradable packaging films provided a natural and safe strategy for food preservation.					
36870661	7	15	theme	packaging	1360:1368	arg1	films					1370:1374	food packaging films	1355:1374	food packaging films cultured with natural soil	1355:1401	Furthermore, the biodegradation rate of food packaging films cultured with natural soil was 95 % after 8 days, clarifying the excellent biodegradability of the packaging films for environmental protection.					
36870661	4	16	theme	food	937:940	arg1	preservation					942:953	sustained food preservation	927:953	sustained food preservation	927:953	As such, we further integrated the obtained EOs@PU microcapsules into potato starch to prepare food packaging films for sustained food preservation.					
36870661	5	17	theme	starch-based	983:994	arg1	films					1006:1010	the prepared starch-based packaging films	970:1010	the prepared starch-based packaging films incorporated with EOs@PU microcapsules	970:1049	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	1	18	theme	sustained	188:196	arg1	activity					212:219	sustained antibacterial activity	188:219	sustained antibacterial activity	188:219	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	7	19	theme	biodegradation	1332:1345	arg1	rate					1347:1350	the biodegradation rate	1328:1350	the biodegradation rate of food packaging films cultured with natural soil	1328:1401	Furthermore, the biodegradation rate of food packaging films cultured with natural soil was 95 % after 8 days, clarifying the excellent biodegradability of the packaging films for environmental protection.					
36870661	7	19	theme	biodegradation	1332:1345	arg1	%					1410:1410	95 %	1407:1410	95 %	1407:1410	Furthermore, the biodegradation rate of food packaging films cultured with natural soil was 95 % after 8 days, clarifying the excellent biodegradability of the packaging films for environmental protection.					
36870661	3	20	theme	PU	675:676	arg1	microcapsules					678:690	the constructed EOs@PU microcapsules	655:690	the constructed EOs@PU microcapsules	655:690	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	0	21	theme	sustained	119:127	arg1	preservation					134:145	sustained food preservation	119:145	sustained food preservation	119:145	Biodegradable starch-based packaging films incorporated with polyurethane-encapsulated essential-oil microcapsules for sustained food preservation.					
36870661	2	22	theme	PU	583:584	arg1	microcapsules					586:598	EOs@PU microcapsules	579:598	EOs@PU microcapsules based on interfacial polymerization	579:634	Herein, three essential oils (EOs) were blended to make composite essential oils with a more harmonious aroma and higher antibacterial ability and encapsulated into polyurethane (PU) to form EOs@PU microcapsules based on interfacial polymerization.					
36870661	7	23	theme	food	1355:1358	arg1	films					1370:1374	food packaging films	1355:1374	food packaging films cultured with natural soil	1355:1401	Furthermore, the biodegradation rate of food packaging films cultured with natural soil was 95 % after 8 days, clarifying the excellent biodegradability of the packaging films for environmental protection.					
36870661	1	24	theme	starch-based	154:165	arg1	films					177:181	Novel starch-based packaging films	148:181	Novel starch-based packaging films with sustained antibacterial activity	148:219	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	2	25	theme	EOs	579:581	arg1	microcapsules					586:598	EOs@PU microcapsules	579:598	EOs@PU microcapsules based on interfacial polymerization	579:634	Herein, three essential oils (EOs) were blended to make composite essential oils with a more harmonious aroma and higher antibacterial ability and encapsulated into polyurethane (PU) to form EOs@PU microcapsules based on interfacial polymerization.					
36870661	5	26	theme	EOs	1030:1032	arg1	microcapsules					1037:1049	EOs@PU microcapsules	1030:1049	EOs@PU microcapsules	1030:1049	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	2	27	theme	essential	454:462	arg1	oils					464:467	composite essential oils	444:467	composite essential oils	444:467	Herein, three essential oils (EOs) were blended to make composite essential oils with a more harmonious aroma and higher antibacterial ability and encapsulated into polyurethane (PU) to form EOs@PU microcapsules based on interfacial polymerization.					
36870661	0	28	theme	Biodegradable	0:12	arg1	films					37:41	Biodegradable starch-based packaging films	0:41	Biodegradable starch-based packaging films	0:41	Biodegradable starch-based packaging films incorporated with polyurethane-encapsulated essential-oil microcapsules for sustained food preservation.					
36870661	1	29	with	films	177:181	arg1	activity					212:219	sustained antibacterial activity	188:219	sustained antibacterial activity	188:219	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	5	30	theme	prepared	974:981	arg1	films					1006:1010	the prepared starch-based packaging films	970:1010	the prepared starch-based packaging films incorporated with EOs@PU microcapsules	970:1049	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	2	31	theme	higher	502:507	arg1	ability					523:529	higher antibacterial ability	502:529	higher antibacterial ability	502:529	Herein, three essential oils (EOs) were blended to make composite essential oils with a more harmonious aroma and higher antibacterial ability and encapsulated into polyurethane (PU) to form EOs@PU microcapsules based on interfacial polymerization.					
36870661	6	32	theme	sustained	1198:1206	arg1	ability					1222:1228	a sustained antibacterial ability	1196:1228	a sustained antibacterial ability	1196:1228	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	0	33	theme	packaging	27:35	arg1	films					37:41	Biodegradable starch-based packaging films	0:41	Biodegradable starch-based packaging films	0:41	Biodegradable starch-based packaging films incorporated with polyurethane-encapsulated essential-oil microcapsules for sustained food preservation.					
36870661	5	34	theme	packaging	996:1004	arg1	films					1006:1010	the prepared starch-based packaging films	970:1010	the prepared starch-based packaging films incorporated with EOs@PU microcapsules	970:1049	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	4	35	theme	EOs	851:853	arg1	microcapsules					858:870	the obtained EOs@PU microcapsules	838:870	the obtained EOs@PU microcapsules	838:870	As such, we further integrated the obtained EOs@PU microcapsules into potato starch to prepare food packaging films for sustained food preservation.					
36870661	5	36	theme	excellent	1058:1066	arg1	rate					1080:1083	an excellent UV blocking rate	1055:1083	an excellent UV blocking rate (>90 %)	1055:1091	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	5	36	theme	excellent	1058:1066	arg1	%					1090:1090	>90 %	1086:1090	>90 %	1086:1090	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	4	37	theme	PU	855:856	arg1	microcapsules					858:870	the obtained EOs@PU microcapsules	838:870	the obtained EOs@PU microcapsules	838:870	As such, we further integrated the obtained EOs@PU microcapsules into potato starch to prepare food packaging films for sustained food preservation.					
36870661	0	38	theme	polyurethane-encapsulated	61:85	arg1	microcapsules					101:113	polyurethane-encapsulated essential-oil microcapsules	61:113	polyurethane-encapsulated essential-oil microcapsules for sustained food preservation	61:145	Biodegradable starch-based packaging films incorporated with polyurethane-encapsulated essential-oil microcapsules for sustained food preservation.					
36870661	5	39	theme	blocking	1071:1078	arg1	rate					1080:1083	an excellent UV blocking rate	1055:1083	an excellent UV blocking rate (>90 %)	1055:1091	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	5	39	theme	blocking	1071:1078	arg1	%					1090:1090	>90 %	1086:1090	>90 %	1086:1090	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	1	40	theme	@	319:319	arg1	microcapsules					301:313	polyurethane-encapsulated essential-oil microcapsules	261:313	polyurethane-encapsulated essential-oil microcapsules (EOs@PU)	261:322	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	1	40	theme	@	319:319	arg1	PU					320:321	EOs@PU	316:321	EOs@PU	316:321	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	5	41	theme	PU	1034:1035	arg1	microcapsules					1037:1049	EOs@PU microcapsules	1030:1049	EOs@PU microcapsules	1030:1049	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	3	42	theme	average	724:730	arg1	size					732:735	an average size	721:735	an average size of approximately 3 μm	721:757	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	7	43	theme	packaging	1475:1483	arg1	films					1485:1489	the packaging films	1471:1489	the packaging films for environmental protection	1471:1518	Furthermore, the biodegradation rate of food packaging films cultured with natural soil was 95 % after 8 days, clarifying the excellent biodegradability of the packaging films for environmental protection.					
36870661	5	44	contain	had	1051:1053	arg2	rate					1080:1083	an excellent UV blocking rate	1055:1083	an excellent UV blocking rate (>90 %)	1055:1091	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	5	44	contain	had	1051:1053	arg2	%					1090:1090	>90 %	1086:1090	>90 %	1086:1090	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	5	44	contain	had	1051:1053	arg1	films					1006:1010	the prepared starch-based packaging films	970:1010	the prepared starch-based packaging films incorporated with EOs@PU microcapsules	970:1049	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	5	44	contain	had	1051:1053	arg2	toxicity					1106:1113	low cell toxicity	1097:1113	low cell toxicity	1097:1113	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	8	45	theme	food	1613:1616	arg1	preservation					1618:1629	food preservation	1613:1629	food preservation	1613:1629	As demonstrated, the biodegradable packaging films provided a natural and safe strategy for food preservation.					
36870661	3	46	theme	3 μm	754:757	arg1	size					732:735	an average size	721:735	an average size of approximately 3 μm	721:757	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	6	47	theme	raspberries	1282:1292	arg1	life					1252:1255	the shelf life	1242:1255	the shelf life of fresh blueberries and raspberries	1242:1292	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	1	48	theme	synthetic	342:350	arg1	microcapsules					301:313	polyurethane-encapsulated essential-oil microcapsules	261:313	polyurethane-encapsulated essential-oil microcapsules (EOs@PU)	261:322	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	1	48	theme	synthetic	342:350	arg1	preservative					352:363	an alternative synthetic preservative	327:363	an alternative synthetic preservative for food preservation	327:385	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	7	49	theme	excellent	1441:1449	arg1	biodegradability					1451:1466	the excellent biodegradability	1437:1466	the excellent biodegradability of the packaging films for environmental protection	1437:1518	Furthermore, the biodegradation rate of food packaging films cultured with natural soil was 95 % after 8 days, clarifying the excellent biodegradability of the packaging films for environmental protection.					
36870661	4	50	theme	food	902:905	arg1	films					917:921	food packaging films	902:921	food packaging films	902:921	As such, we further integrated the obtained EOs@PU microcapsules into potato starch to prepare food packaging films for sustained food preservation.					
36870661	6	51	theme	packaging	1180:1188	arg1	films					1190:1194	the packaging films	1176:1194	the packaging films	1176:1194	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	6	52	theme	blueberries	1266:1276	arg1	life					1252:1255	the shelf life	1242:1255	the shelf life of fresh blueberries and raspberries	1242:1292	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	3	53	theme	high	774:777	arg1	%					803:803	59.01 %	797:803	59.01 %	797:803	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	3	53	theme	high	774:777	arg1	capacity					787:794	high loading capacity	774:794	high loading capacity (59.01 %)	774:804	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	2	54	theme	essential	402:410	arg1	EOs					418:420	EOs	418:420	EOs	418:420	Herein, three essential oils (EOs) were blended to make composite essential oils with a more harmonious aroma and higher antibacterial ability and encapsulated into polyurethane (PU) to form EOs@PU microcapsules based on interfacial polymerization.					
36870661	2	54	theme	essential	402:410	arg1	oils					412:415	three essential oils	396:415	three essential oils (EOs)	396:421	Herein, three essential oils (EOs) were blended to make composite essential oils with a more harmonious aroma and higher antibacterial ability and encapsulated into polyurethane (PU) to form EOs@PU microcapsules based on interfacial polymerization.					
36870661	4	55	theme	potato	877:882	arg1	starch					884:889	potato starch	877:889	potato starch	877:889	As such, we further integrated the obtained EOs@PU microcapsules into potato starch to prepare food packaging films for sustained food preservation.					
36870661	6	56	theme	PU	1154:1155	arg1	microcapsules					1157:1169	EOs@PU microcapsules	1150:1169	EOs@PU microcapsules	1150:1169	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	1	57	theme	essential-oil	287:299	arg1	microcapsules					301:313	polyurethane-encapsulated essential-oil microcapsules	261:313	polyurethane-encapsulated essential-oil microcapsules (EOs@PU)	261:322	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	1	57	theme	essential-oil	287:299	arg1	PU					320:321	EOs@PU	316:321	EOs@PU	316:321	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	1	57	theme	essential-oil	287:299	arg1	preservative					352:363	an alternative synthetic preservative	327:363	an alternative synthetic preservative for food preservation	327:385	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	6	58	theme	EOs	1150:1152	arg1	microcapsules					1157:1169	EOs@PU microcapsules	1150:1169	EOs@PU microcapsules	1150:1169	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	3	59	theme	constructed	659:669	arg1	microcapsules					678:690	the constructed EOs@PU microcapsules	655:690	the constructed EOs@PU microcapsules	655:690	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	5	60	theme	cell	1101:1104	arg1	toxicity					1106:1113	low cell toxicity	1097:1113	low cell toxicity	1097:1113	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	1	61	theme	packaging	167:175	arg1	films					177:181	Novel starch-based packaging films	148:181	Novel starch-based packaging films with sustained antibacterial activity	148:219	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	7	62	theme	films	1370:1374	arg1	rate					1347:1350	the biodegradation rate	1328:1350	the biodegradation rate of food packaging films cultured with natural soil	1328:1401	Furthermore, the biodegradation rate of food packaging films cultured with natural soil was 95 % after 8 days, clarifying the excellent biodegradability of the packaging films for environmental protection.					
36870661	7	62	theme	films	1370:1374	arg1	%					1410:1410	95 %	1407:1410	95 %	1407:1410	Furthermore, the biodegradation rate of food packaging films cultured with natural soil was 95 % after 8 days, clarifying the excellent biodegradability of the packaging films for environmental protection.					
36870661	6	63	theme	>	1304:1304	arg1	7 days					1306:1311	> 7 days	1304:1311	> 7 days	1304:1311	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	6	63	theme	>	1304:1304	arg1	25 °C					1297:1301	25 °C	1297:1301	25 °C (> 7 days)	1297:1312	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	3	64	theme	microcapsules	678:690	arg1	regular					696:702	regular	696:702	regular	696:702	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	3	64	theme	microcapsules	678:690	arg1	morphology					641:650	The morphology	637:650	The morphology of the constructed EOs@PU microcapsules	637:690	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	7	65	theme	natural	1390:1396	arg1	soil					1398:1401	natural soil	1390:1401	natural soil	1390:1401	Furthermore, the biodegradation rate of food packaging films cultured with natural soil was 95 % after 8 days, clarifying the excellent biodegradability of the packaging films for environmental protection.					
36870661	4	66	theme	sustained	927:935	arg1	preservation					942:953	sustained food preservation	927:953	sustained food preservation	927:953	As such, we further integrated the obtained EOs@PU microcapsules into potato starch to prepare food packaging films for sustained food preservation.					
36870661	6	67	theme	antibacterial	1208:1220	arg1	ability					1222:1228	a sustained antibacterial ability	1196:1228	a sustained antibacterial ability	1196:1228	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	3	68	theme	@	674:674	arg1	microcapsules					678:690	the constructed EOs@PU microcapsules	655:690	the constructed EOs@PU microcapsules	655:690	The morphology of the constructed EOs@PU microcapsules was regular and uniform with an average size of approximately 3 μm, thus enabling high loading capacity (59.01 %).					
36870661	1	69	theme	antibacterial	198:210	arg1	activity					212:219	sustained antibacterial activity	188:219	sustained antibacterial activity	188:219	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	0	70	theme	food	129:132	arg1	preservation					134:145	sustained food preservation	119:145	sustained food preservation	119:145	Biodegradable starch-based packaging films incorporated with polyurethane-encapsulated essential-oil microcapsules for sustained food preservation.					
36870661	2	71	theme	@	582:582	arg1	microcapsules					586:598	EOs@PU microcapsules	579:598	EOs@PU microcapsules based on interfacial polymerization	579:634	Herein, three essential oils (EOs) were blended to make composite essential oils with a more harmonious aroma and higher antibacterial ability and encapsulated into polyurethane (PU) to form EOs@PU microcapsules based on interfacial polymerization.					
36870661	0	72	theme	starch-based	14:25	arg1	films					37:41	Biodegradable starch-based packaging films	0:41	Biodegradable starch-based packaging films	0:41	Biodegradable starch-based packaging films incorporated with polyurethane-encapsulated essential-oil microcapsules for sustained food preservation.					
36870661	2	73	theme	antibacterial	509:521	arg1	ability					523:529	higher antibacterial ability	502:529	higher antibacterial ability	502:529	Herein, three essential oils (EOs) were blended to make composite essential oils with a more harmonious aroma and higher antibacterial ability and encapsulated into polyurethane (PU) to form EOs@PU microcapsules based on interfacial polymerization.					
36870661	8	74	theme	packaging	1556:1564	arg1	films					1566:1570	the biodegradable packaging films	1538:1570	the biodegradable packaging films	1538:1570	As demonstrated, the biodegradable packaging films provided a natural and safe strategy for food preservation.					
36870661	2	75	theme	composite	444:452	arg1	oils					464:467	composite essential oils	444:467	composite essential oils	444:467	Herein, three essential oils (EOs) were blended to make composite essential oils with a more harmonious aroma and higher antibacterial ability and encapsulated into polyurethane (PU) to form EOs@PU microcapsules based on interfacial polymerization.					
36870661	1	76	theme	food	369:372	arg1	preservation					374:385	food preservation	369:385	food preservation	369:385	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	1	77	theme	Novel	148:152	arg1	films					177:181	Novel starch-based packaging films	148:181	Novel starch-based packaging films with sustained antibacterial activity	148:219	Novel starch-based packaging films with sustained antibacterial activity were successfully made by incorporating polyurethane-encapsulated essential-oil microcapsules (EOs@PU) as an alternative synthetic preservative for food preservation.					
36870661	2	78	theme	harmonious	481:490	arg1	aroma					492:496	a more harmonious aroma	474:496	a more harmonious aroma	474:496	Herein, three essential oils (EOs) were blended to make composite essential oils with a more harmonious aroma and higher antibacterial ability and encapsulated into polyurethane (PU) to form EOs@PU microcapsules based on interfacial polymerization.					
36870661	8	79	theme	natural	1583:1589	arg1	strategy					1600:1607	a natural and safe strategy	1581:1607	a natural and safe strategy for food preservation	1581:1629	As demonstrated, the biodegradable packaging films provided a natural and safe strategy for food preservation.					
36870661	4	80	theme	obtained	842:849	arg1	microcapsules					858:870	the obtained EOs@PU microcapsules	838:870	the obtained EOs@PU microcapsules	838:870	As such, we further integrated the obtained EOs@PU microcapsules into potato starch to prepare food packaging films for sustained food preservation.					
36870661	0	81	theme	essential-oil	87:99	arg1	microcapsules					101:113	polyurethane-encapsulated essential-oil microcapsules	61:113	polyurethane-encapsulated essential-oil microcapsules for sustained food preservation	61:145	Biodegradable starch-based packaging films incorporated with polyurethane-encapsulated essential-oil microcapsules for sustained food preservation.					
36870661	5	82	theme	UV	1068:1069	arg1	rate					1080:1083	an excellent UV blocking rate	1055:1083	an excellent UV blocking rate (>90 %)	1055:1091	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	5	82	theme	UV	1068:1069	arg1	%					1090:1090	>90 %	1086:1090	>90 %	1086:1090	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	8	83	theme	safe	1595:1598	arg1	strategy					1600:1607	a natural and safe strategy	1581:1607	a natural and safe strategy for food preservation	1581:1629	As demonstrated, the biodegradable packaging films provided a natural and safe strategy for food preservation.					
36870661	4	84	theme	@	854:854	arg1	microcapsules					858:870	the obtained EOs@PU microcapsules	838:870	the obtained EOs@PU microcapsules	838:870	As such, we further integrated the obtained EOs@PU microcapsules into potato starch to prepare food packaging films for sustained food preservation.					
36870661	5	85	theme	@	1033:1033	arg1	microcapsules					1037:1049	EOs@PU microcapsules	1030:1049	EOs@PU microcapsules	1030:1049	Consequently, the prepared starch-based packaging films incorporated with EOs@PU microcapsules had an excellent UV blocking rate (>90 %) and low cell toxicity.					
36870661	6	86	theme	long-term	1129:1137	arg1	release					1139:1145	the long-term release	1125:1145	the long-term release of EOs@PU microcapsules	1125:1169	Notably, the long-term release of EOs@PU microcapsules gave the packaging films a sustained antibacterial ability, prolonging the shelf life of fresh blueberries and raspberries at 25 °C (> 7 days).					
36870661	7	87	theme	environmental	1495:1507	arg1	protection					1509:1518	environmental protection	1495:1518	environmental protection	1495:1518	Furthermore, the biodegradation rate of food packaging films cultured with natural soil was 95 % after 8 days, clarifying the excellent biodegradability of the packaging films for environmental protection.					
36801220	0	0	theme	aqueous	87:93	arg1	solutions					95:103	aqueous solutions	87:103	aqueous solutions	87:103	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	2	1	theme	methylene	510:518	arg1	dyes					553:556	methylene blue (MB) and methyl orange (MO) dyes	510:556	methylene blue (MB) and methyl orange (MO) dyes	510:556	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	2	theme	carbon	389:394	arg1	CPML					437:440	CPML	437:440	CPML	437:440	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	2	theme	carbon	389:394	arg1	hydroxide					426:434	multi-walled carbon nanotube-Mg Al-layered double hydroxide	376:434	multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML)	376:441	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	6	3	theme	different	930:938	arg1	isotherm					940:947	different isotherm	930:947	different isotherm	930:947	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	4	4	theme	pH	800:801	arg1	factors					803:809	pH factors	800:809	pH factors	800:809	Response surface methodology (RSM) was utilized to evaluate dye removal based on the initial concentration, dosage, and pH factors.					
36801220	1	5	from	existence	157:165	arg1	environment					209:219	the water environment	199:219	the water environment developing new and effective techniques to remove them simultaneously	199:289	Regarding the existence of cationic and anionic dyes in the water environment developing new and effective techniques to remove them simultaneously is essential.					
36801220	2	6	theme	multi-walled	376:387	arg1	CPML					437:440	CPML	437:440	CPML	437:440	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	6	theme	multi-walled	376:387	arg1	hydroxide					426:434	multi-walled carbon nanotube-Mg Al-layered double hydroxide	376:434	multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML)	376:441	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	8	7	theme	dye-contaminated	1380:1395	arg1	water					1397:1401	cationic and anionic dye-contaminated water	1359:1401	cationic and anionic dye-contaminated water	1359:1401	Experimental results show that the CPML NC has sufficient potential for treating cationic and anionic dye-contaminated water.					
36801220	2	8	theme	methyl	534:539	arg1	MO					549:550	MO	549:550	MO	549:550	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	8	theme	methyl	534:539	arg1	orange					541:546	methyl orange	534:546	methyl orange (MO)	534:551	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	6	9	theme	monolayer	1128:1136	arg1	manner					1149:1154	a monolayer adsorption manner	1126:1154	a monolayer adsorption manner	1126:1154	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	5	10	theme	adsorption	824:833	arg1	capacities					835:844	The highest adsorption capacities	812:844	The highest adsorption capacities	812:844	The highest adsorption capacities were measured at 471.12 and 230.87 mg g-1 for MB and MO, respectively.					
36801220	6	11	theme	pseudo-second-order	1075:1093	arg1	model					1103:1107	the Langmuir and pseudo-second-order kinetic model	1058:1107	model	1103:1107	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	2	12	theme	aquatic	575:581	arg1	medium					583:588	the aquatic medium	571:588	the aquatic medium	571:588	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	7	13	theme	reusability	1195:1205	arg1	experiment					1207:1216	The reusability experiment	1191:1216	The reusability experiment clarified that the CPML NC	1191:1243	The reusability experiment clarified that the CPML NC could be applied multiple times.					
36801220	6	14	theme	NCs	1186:1188	arg1	surface					1175:1181	the homogeneous surface	1159:1181	the homogeneous surface of NCs	1159:1188	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	1	15	theme	cationic	170:177	arg1	dyes					191:194	cationic and anionic dyes	170:194	cationic and anionic dyes	170:194	Regarding the existence of cationic and anionic dyes in the water environment developing new and effective techniques to remove them simultaneously is essential.					
36801220	3	16	theme	synthesized	662:672	arg1	CPML					674:677	the synthesized CPML	658:677	the synthesized CPML	658:677	The SEM, TGA, FTIR, XRD, and BET methods were used to characterize the synthesized CPML.					
36801220	0	17	theme	cationic	41:48	arg1	dyes					62:65	cationic and anionic dyes	41:65	cationic and anionic dyes	41:65	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	3	18	used	used	637:640	arg2	methods					624:630	The SEM, TGA, FTIR, XRD, and BET methods	591:630	The SEM, TGA, FTIR, XRD, and BET methods	591:630	The SEM, TGA, FTIR, XRD, and BET methods were used to characterize the synthesized CPML.					
36801220	6	19	theme	dyes	1004:1007	arg1	adsorption					986:995	the adsorption	982:995	the adsorption of the dyes onto CPML nanocomposite (NC)	982:1036	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	0	20	theme	composite	15:23	arg1	films					25:29	Chitosan-based composite films	0:29	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.	0:141	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	0	20	theme	composite	15:23	arg1	optimization					119:130	optimization	119:130	optimization	119:130	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	0	20	theme	composite	15:23	arg1	Modeling					106:113	Modeling	106:113	Modeling	106:113	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	2	21	used	used	475:478	arg2	removal					558:564	an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal	483:564	an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium	483:588	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	21	used	used	475:478	arg2	film					355:358	a chitosan/poly-2-aminothiazole composite film	313:358	a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML)	313:441	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	22	theme	chitosan/poly-2-aminothiazole	315:343	arg1	removal					558:564	an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal	483:564	an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium	483:588	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	22	theme	chitosan/poly-2-aminothiazole	315:343	arg1	film					355:358	a chitosan/poly-2-aminothiazole composite film	313:358	a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML)	313:441	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	1	23	theme	anionic	183:189	arg1	dyes					191:194	cationic and anionic dyes	170:194	cationic and anionic dyes	170:194	Regarding the existence of cationic and anionic dyes in the water environment developing new and effective techniques to remove them simultaneously is essential.					
36801220	0	24	theme	Chitosan-based	0:13	arg1	films					25:29	Chitosan-based composite films	0:29	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.	0:141	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	0	24	theme	Chitosan-based	0:13	arg1	optimization					119:130	optimization	119:130	optimization	119:130	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	0	24	theme	Chitosan-based	0:13	arg1	Modeling					106:113	Modeling	106:113	Modeling	106:113	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	2	25	from	medium	583:588	arg1	removal					558:564	an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal	483:564	an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium	483:588	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	25	from	medium	583:588	arg1	film					355:358	a chitosan/poly-2-aminothiazole composite film	313:358	a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML)	313:441	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	4	26	theme	surface	689:695	arg1	RSM					710:712	RSM	710:712	RSM	710:712	Response surface methodology (RSM) was utilized to evaluate dye removal based on the initial concentration, dosage, and pH factors.					
36801220	4	26	theme	surface	689:695	arg1	methodology					697:707	Response surface methodology	680:707	Response surface methodology (RSM)	680:713	Response surface methodology (RSM) was utilized to evaluate dye removal based on the initial concentration, dosage, and pH factors.					
36801220	1	27	theme	dyes	191:194	arg1	existence					157:165	the existence	153:165	the existence of cationic and anionic dyes in the water environment developing new and effective techniques to remove them simultaneously	153:289	Regarding the existence of cationic and anionic dyes in the water environment developing new and effective techniques to remove them simultaneously is essential.					
36801220	8	28	theme	sufficient	1325:1334	arg1	potential					1336:1344	sufficient potential	1325:1344	sufficient potential	1325:1344	Experimental results show that the CPML NC has sufficient potential for treating cationic and anionic dye-contaminated water.					
36801220	8	29	theme	CPML	1313:1316	arg1	NC					1318:1319	the CPML NC	1309:1319	the CPML NC	1309:1319	Experimental results show that the CPML NC has sufficient potential for treating cationic and anionic dye-contaminated water.					
36801220	7	30	theme	CPML	1237:1240	arg1	NC					1242:1243	the CPML NC	1233:1243	the CPML NC	1233:1243	The reusability experiment clarified that the CPML NC could be applied multiple times.					
36801220	3	31	theme	XRD	611:613	arg1	methods					624:630	The SEM, TGA, FTIR, XRD, and BET methods	591:630	The SEM, TGA, FTIR, XRD, and BET methods	591:630	The SEM, TGA, FTIR, XRD, and BET methods were used to characterize the synthesized CPML.					
36801220	6	32	theme	CPML	1014:1017	arg1	NC					1034:1035	NC	1034:1035	NC	1034:1035	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	6	32	theme	CPML	1014:1017	arg1	nanocomposite					1019:1031	CPML nanocomposite	1014:1031	CPML nanocomposite (NC)	1014:1036	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	5	33	theme	highest	816:822	arg1	capacities					835:844	The highest adsorption capacities	812:844	The highest adsorption capacities	812:844	The highest adsorption capacities were measured at 471.12 and 230.87 mg g-1 for MB and MO, respectively.					
36801220	2	34	theme	adsorbent	496:504	arg1	removal					558:564	an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal	483:564	an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium	483:588	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	34	theme	adsorbent	496:504	arg1	film					355:358	a chitosan/poly-2-aminothiazole composite film	313:358	a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML)	313:441	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	8	35	theme	anionic	1372:1378	arg1	water					1397:1401	cationic and anionic dye-contaminated water	1359:1401	cationic and anionic dye-contaminated water	1359:1401	Experimental results show that the CPML NC has sufficient potential for treating cationic and anionic dye-contaminated water.					
36801220	6	36	theme	homogeneous	1163:1173	arg1	surface					1175:1181	the homogeneous surface	1159:1181	the homogeneous surface of NCs	1159:1188	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	2	37	theme	double	419:424	arg1	CPML					437:440	CPML	437:440	CPML	437:440	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	37	theme	double	419:424	arg1	hydroxide					426:434	multi-walled carbon nanotube-Mg Al-layered double hydroxide	376:434	multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML)	376:441	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	1	38	theme	water	203:207	arg1	environment					209:219	the water environment	199:219	the water environment developing new and effective techniques to remove them simultaneously	199:289	Regarding the existence of cationic and anionic dyes in the water environment developing new and effective techniques to remove them simultaneously is essential.					
36801220	2	39	theme	effective	486:494	arg1	removal					558:564	an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal	483:564	an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium	483:588	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	39	theme	effective	486:494	arg1	film					355:358	a chitosan/poly-2-aminothiazole composite film	313:358	a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML)	313:441	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	40	theme	orange	541:546	arg1	dyes					553:556	methylene blue (MB) and methyl orange (MO) dyes	510:556	methylene blue (MB) and methyl orange (MO) dyes	510:556	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	3	41	theme	FTIR	605:608	arg1	methods					624:630	The SEM, TGA, FTIR, XRD, and BET methods	591:630	The SEM, TGA, FTIR, XRD, and BET methods	591:630	The SEM, TGA, FTIR, XRD, and BET methods were used to characterize the synthesized CPML.					
36801220	0	42	theme	anionic	54:60	arg1	dyes					62:65	cationic and anionic dyes	41:65	cationic and anionic dyes	41:65	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	8	43	contain	has	1321:1323	arg1	NC					1318:1319	the CPML NC	1309:1319	the CPML NC	1309:1319	Experimental results show that the CPML NC has sufficient potential for treating cationic and anionic dye-contaminated water.					
36801220	8	43	contain	has	1321:1323	arg2	potential					1336:1344	sufficient potential	1325:1344	sufficient potential	1325:1344	Experimental results show that the CPML NC has sufficient potential for treating cationic and anionic dye-contaminated water.					
36801220	4	44	theme	initial	765:771	arg1	concentration					773:785	the initial concentration	761:785	the initial concentration	761:785	Response surface methodology (RSM) was utilized to evaluate dye removal based on the initial concentration, dosage, and pH factors.					
36801220	4	45	used	utilized	719:726	arg2	RSM					710:712	RSM	710:712	RSM	710:712	Response surface methodology (RSM) was utilized to evaluate dye removal based on the initial concentration, dosage, and pH factors.					
36801220	4	45	used	utilized	719:726	arg2	methodology					697:707	Response surface methodology	680:707	Response surface methodology (RSM)	680:713	Response surface methodology (RSM) was utilized to evaluate dye removal based on the initial concentration, dosage, and pH factors.					
36801220	8	46	theme	cationic	1359:1366	arg1	water					1397:1401	cationic and anionic dye-contaminated water	1359:1401	cationic and anionic dye-contaminated water	1359:1401	Experimental results show that the CPML NC has sufficient potential for treating cationic and anionic dye-contaminated water.					
36801220	4	47	theme	dye	740:742	arg1	removal					744:750	dye removal	740:750	dye removal	740:750	Response surface methodology (RSM) was utilized to evaluate dye removal based on the initial concentration, dosage, and pH factors.					
36801220	3	48	theme	TGA	600:602	arg1	methods					624:630	The SEM, TGA, FTIR, XRD, and BET methods	591:630	The SEM, TGA, FTIR, XRD, and BET methods	591:630	The SEM, TGA, FTIR, XRD, and BET methods were used to characterize the synthesized CPML.					
36801220	6	49	theme	kinetic	953:959	arg1	models					961:966	kinetic models	953:966	kinetic models	953:966	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	4	50	theme	Response	680:687	arg1	RSM					710:712	RSM	710:712	RSM	710:712	Response surface methodology (RSM) was utilized to evaluate dye removal based on the initial concentration, dosage, and pH factors.					
36801220	4	50	theme	Response	680:687	arg1	methodology					697:707	Response surface methodology	680:707	Response surface methodology (RSM)	680:713	Response surface methodology (RSM) was utilized to evaluate dye removal based on the initial concentration, dosage, and pH factors.					
36801220	8	51	theme	Experimental	1278:1289	arg1	results					1291:1297	Experimental results	1278:1297	Experimental results	1278:1297	Experimental results show that the CPML NC has sufficient potential for treating cationic and anionic dye-contaminated water.					
36801220	6	52	theme	models	961:966	arg1	study					921:925	The study	917:925	The study of different isotherm and kinetic models	917:966	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	6	53	theme	kinetic	1095:1101	arg1	model					1103:1107	the Langmuir and pseudo-second-order kinetic model	1058:1107	model	1103:1107	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	6	54	theme	adsorption	1138:1147	arg1	manner					1149:1154	a monolayer adsorption manner	1126:1154	a monolayer adsorption manner	1126:1154	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	0	55	dep	films	25:29	arg1	films					25:29	Chitosan-based composite films	0:29	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.	0:141	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	0	55	dep	films	25:29	arg1	optimization					119:130	optimization	119:130	optimization	119:130	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	0	55	dep	films	25:29	arg1	Modeling					106:113	Modeling	106:113	Modeling	106:113	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	0	55	dep	films	25:29	arg1	remove					34:39	remove	34:39	to remove cationic and anionic dyes simultaneously from aqueous solutions	31:103	Chitosan-based composite films to remove cationic and anionic dyes simultaneously from aqueous solutions: Modeling and optimization using RSM.					
36801220	2	56	theme	composite	345:353	arg1	removal					558:564	an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal	483:564	an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium	483:588	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	56	theme	composite	345:353	arg1	film					355:358	a chitosan/poly-2-aminothiazole composite film	313:358	a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML)	313:441	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	1	57	theme	new	232:234	arg1	techniques					250:259	new and effective techniques	232:259	new and effective techniques	232:259	Regarding the existence of cationic and anionic dyes in the water environment developing new and effective techniques to remove them simultaneously is essential.					
36801220	3	58	theme	BET	620:622	arg1	methods					624:630	The SEM, TGA, FTIR, XRD, and BET methods	591:630	The SEM, TGA, FTIR, XRD, and BET methods	591:630	The SEM, TGA, FTIR, XRD, and BET methods were used to characterize the synthesized CPML.					
36801220	3	59	theme	SEM	595:597	arg1	methods					624:630	The SEM, TGA, FTIR, XRD, and BET methods	591:630	The SEM, TGA, FTIR, XRD, and BET methods	591:630	The SEM, TGA, FTIR, XRD, and BET methods were used to characterize the synthesized CPML.					
36801220	2	60	theme	Al-layered	408:417	arg1	CPML					437:440	CPML	437:440	CPML	437:440	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	60	theme	Al-layered	408:417	arg1	hydroxide					426:434	multi-walled carbon nanotube-Mg Al-layered double hydroxide	376:434	multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML)	376:441	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	7	61	theme	multiple	1262:1269	arg1	times					1271:1275	multiple times	1262:1275	multiple times	1262:1275	The reusability experiment clarified that the CPML NC could be applied multiple times.					
36801220	2	62	theme	blue	520:523	arg1	methylene					510:518	methylene blue	510:523	methylene blue (MB)	510:528	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	62	theme	blue	520:523	arg1	MB					526:527	MB	526:527	MB	526:527	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	6	63	theme	isotherm	940:947	arg1	study					921:925	The study	917:925	The study of different isotherm and kinetic models	917:966	The study of different isotherm and kinetic models revealed that the adsorption of the dyes onto CPML nanocomposite (NC) was correlated with the Langmuir and pseudo-second-order kinetic model, which indicated a monolayer adsorption manner on the homogeneous surface of NCs.					
36801220	2	64	theme	nanotube-Mg	396:406	arg1	CPML					437:440	CPML	437:440	CPML	437:440	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	2	64	theme	nanotube-Mg	396:406	arg1	hydroxide					426:434	multi-walled carbon nanotube-Mg Al-layered double hydroxide	376:434	multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML)	376:441	Herein, a chitosan/poly-2-aminothiazole composite film reinforced with multi-walled carbon nanotube-Mg Al-layered double hydroxide (CPML) was created, characterized, and used as an effective adsorbent for methylene blue (MB) and methyl orange (MO) dyes removal from the aquatic medium.					
36801220	1	65	theme	effective	240:248	arg1	techniques					250:259	new and effective techniques	232:259	new and effective techniques	232:259	Regarding the existence of cationic and anionic dyes in the water environment developing new and effective techniques to remove them simultaneously is essential.					
37268080	5	0	theme	chemoattractant	1143:1157	arg1	MCP-1					1170:1174	MCP-1	1170:1174	MCP-1	1170:1174	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	5	0	theme	chemoattractant	1143:1157	arg1	protein-1					1159:1167	chemoattractant protein-1	1143:1167	chemoattractant protein-1 (MCP-1)	1143:1175	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	1	1	theme	factors	253:259	arg1	overproduction					218:231	the overproduction	214:231	the overproduction of pro-inflammatory factors and reactive oxygen species (ROS)	214:293	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
37268080	0	2	theme	anti-inflammation	73:89	arg1	therapy					91:97	anti-inflammation therapy	73:97	anti-inflammation therapy	73:97	Composite nanofibrous dressing loaded with Prussian blue and heparin for anti-inflammation therapy and diabetic wound healing.					
37268080	6	3	theme	wound	1383:1387	arg1	healing					1389:1395	wound healing	1383:1395	wound healing	1383:1395	Furthermore, a chronic wound healing evaluation conducted in vivo demonstrated that the PPBDH dressing was able to effectively alleviate the inflammatory response and accelerate wound healing.					
37268080	7	4	contain	have	1502:1505	arg2	potential					1513:1521	great potential	1507:1521	great potential	1507:1521	This research presents an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation.					
37268080	7	4	contain	have	1502:1505	arg1	dressings					1485:1493	nanozyme hybrid nanofibrous dressings	1457:1493	nanozyme hybrid nanofibrous dressings	1457:1493	This research presents an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation.					
37268080	0	5	theme	diabetic	103:110	arg1	healing					118:124	diabetic wound healing	103:124	diabetic wound healing	103:124	Composite nanofibrous dressing loaded with Prussian blue and heparin for anti-inflammation therapy and diabetic wound healing.					
37268080	6	6	theme	PPBDH	1293:1297	arg1	able					1312:1315	able	1312:1315	able	1312:1315	Furthermore, a chronic wound healing evaluation conducted in vivo demonstrated that the PPBDH dressing was able to effectively alleviate the inflammatory response and accelerate wound healing.					
37268080	6	6	theme	PPBDH	1293:1297	arg1	dressing					1299:1306	the PPBDH dressing	1289:1306	the PPBDH dressing	1289:1306	Furthermore, a chronic wound healing evaluation conducted in vivo demonstrated that the PPBDH dressing was able to effectively alleviate the inflammatory response and accelerate wound healing.					
37268080	6	7	theme	chronic	1220:1226	arg1	healing					1234:1240	a chronic wound healing	1218:1240	a chronic wound healing evaluation conducted in vivo	1218:1269	Furthermore, a chronic wound healing evaluation conducted in vivo demonstrated that the PPBDH dressing was able to effectively alleviate the inflammatory response and accelerate wound healing.					
37268080	1	8	theme	reactive	265:272	arg1	ROS					290:292	ROS	290:292	ROS	290:292	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
37268080	1	8	theme	reactive	265:272	arg1	species					281:287	reactive oxygen species	265:287	reactive oxygen species (ROS)	265:293	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
37268080	4	9	theme	PBNCs	948:952	arg1	levels					938:943	the enzyme-like activity levels	913:943	the enzyme-like activity levels of PBNCs	913:952	It is worth noting that the nanozymes were firmly anchored to the fiber surfaces through slight polymer swelling caused by the solvent during electrospinning, thereby guaranteeing the preservation of the enzyme-like activity levels of PBNCs.					
37268080	5	10	theme	excessive	1097:1105	arg1	factors					1124:1130	excessive pro-inflammatory factors	1097:1130	excessive pro-inflammatory factors	1097:1130	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	5	10	theme	excessive	1097:1105	arg1	interleukin-1β					1181:1194	interleukin-1β	1181:1194	interleukin-1β (IL-1β)	1181:1202	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	5	10	theme	excessive	1097:1105	arg1	protein-1					1159:1167	chemoattractant protein-1	1143:1167	chemoattractant protein-1 (MCP-1)	1143:1175	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	7	11	with	healing	1543:1549	arg1	inflammation					1602:1613	uncontrolled inflammation	1589:1613	uncontrolled inflammation	1589:1613	This research presents an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation.					
37268080	1	12	theme	oxygen	274:279	arg1	ROS					290:292	ROS	290:292	ROS	290:292	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
37268080	1	12	theme	oxygen	274:279	arg1	species					281:287	reactive oxygen species	265:287	reactive oxygen species (ROS)	265:293	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
37268080	5	13	from	apoptosis	1072:1080	arg1	cells					1049:1053	cells	1049:1053	cells from ROS-induced apoptosis	1049:1080	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	5	14	theme	pro-inflammatory	1107:1122	arg1	factors					1124:1130	excessive pro-inflammatory factors	1097:1130	excessive pro-inflammatory factors	1097:1130	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	5	14	theme	pro-inflammatory	1107:1122	arg1	interleukin-1β					1181:1194	interleukin-1β	1181:1194	interleukin-1β (IL-1β)	1181:1202	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	5	14	theme	pro-inflammatory	1107:1122	arg1	protein-1					1159:1167	chemoattractant protein-1	1143:1167	chemoattractant protein-1 (MCP-1)	1143:1175	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	7	15	theme	nanozyme	1457:1464	arg1	dressings					1485:1493	nanozyme hybrid nanofibrous dressings	1457:1493	nanozyme hybrid nanofibrous dressings	1457:1493	This research presents an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation.					
37268080	1	16	theme	diabetes	170:177	arg1	ulcer					136:140	Diabetic ulcer	127:140	Diabetic ulcer	127:140	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
37268080	1	16	theme	diabetes	170:177	arg1	complication					154:165	a severe complication	145:165	a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS)	145:293	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
37268080	0	17	theme	wound	112:116	arg1	healing					118:124	diabetic wound healing	103:124	diabetic wound healing	103:124	Composite nanofibrous dressing loaded with Prussian blue and heparin for anti-inflammation therapy and diabetic wound healing.					
37268080	7	18	theme	hybrid	1466:1471	arg1	dressings					1485:1493	nanozyme hybrid nanofibrous dressings	1457:1493	nanozyme hybrid nanofibrous dressings	1457:1493	This research presents an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation.					
37268080	2	19	theme	blue	380:383	arg1	PBNCs					399:403	PBNCs	399:403	PBNCs	399:403	In this study, a composite nanofibrous dressing was developed by combining Prussian blue nanocrystals (PBNCs) and heparin sodium (Hep) through electrospinning, electrospraying, and chemical deposition.					
37268080	2	19	theme	blue	380:383	arg1	nanocrystals					385:396	Prussian blue nanocrystals	371:396	Prussian blue nanocrystals (PBNCs)	371:404	In this study, a composite nanofibrous dressing was developed by combining Prussian blue nanocrystals (PBNCs) and heparin sodium (Hep) through electrospinning, electrospraying, and chemical deposition.					
37268080	0	20	theme	nanofibrous	10:20	arg1	dressing					22:29	Composite nanofibrous dressing	0:29	Composite nanofibrous dressing	0:29	Composite nanofibrous dressing loaded with Prussian blue and heparin for anti-inflammation therapy and diabetic wound healing.					
37268080	7	21	theme	great	1507:1511	arg1	potential					1513:1521	great potential	1507:1521	great potential	1507:1521	This research presents an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation.					
37268080	2	22	theme	Prussian	371:378	arg1	PBNCs					399:403	PBNCs	399:403	PBNCs	399:403	In this study, a composite nanofibrous dressing was developed by combining Prussian blue nanocrystals (PBNCs) and heparin sodium (Hep) through electrospinning, electrospraying, and chemical deposition.					
37268080	2	22	theme	Prussian	371:378	arg1	nanocrystals					385:396	Prussian blue nanocrystals	371:396	Prussian blue nanocrystals (PBNCs)	371:404	In this study, a composite nanofibrous dressing was developed by combining Prussian blue nanocrystals (PBNCs) and heparin sodium (Hep) through electrospinning, electrospraying, and chemical deposition.					
37268080	0	23	theme	Composite	0:8	arg1	dressing					22:29	Composite nanofibrous dressing	0:29	Composite nanofibrous dressing	0:29	Composite nanofibrous dressing loaded with Prussian blue and heparin for anti-inflammation therapy and diabetic wound healing.					
37268080	1	24	theme	species	281:287	arg1	overproduction					218:231	the overproduction	214:231	the overproduction of pro-inflammatory factors and reactive oxygen species (ROS)	214:293	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
37268080	7	25	theme	chronic	1554:1560	arg1	wounds					1577:1582	chronic and refractory wounds	1554:1582	chronic and refractory wounds	1554:1582	This research presents an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation.					
37268080	7	26	theme	uncontrolled	1589:1600	arg1	inflammation					1602:1613	uncontrolled inflammation	1589:1613	uncontrolled inflammation	1589:1613	This research presents an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation.					
37268080	4	27	theme	activity	929:936	arg1	levels					938:943	the enzyme-like activity levels	913:943	the enzyme-like activity levels of PBNCs	913:952	It is worth noting that the nanozymes were firmly anchored to the fiber surfaces through slight polymer swelling caused by the solvent during electrospinning, thereby guaranteeing the preservation of the enzyme-like activity levels of PBNCs.					
37268080	7	28	theme	innovative	1424:1433	arg1	approach					1435:1442	an innovative approach	1421:1442	an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation	1421:1613	This research presents an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation.					
37268080	5	29	theme	intracellular	1012:1024	arg1	levels					1030:1035	intracellular ROS levels	1012:1035	intracellular ROS levels	1012:1035	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	2	30	theme	heparin	410:416	arg1	Hep					426:428	Hep	426:428	Hep	426:428	In this study, a composite nanofibrous dressing was developed by combining Prussian blue nanocrystals (PBNCs) and heparin sodium (Hep) through electrospinning, electrospraying, and chemical deposition.					
37268080	2	30	theme	heparin	410:416	arg1	sodium					418:423	heparin sodium	410:423	heparin sodium (Hep)	410:429	In this study, a composite nanofibrous dressing was developed by combining Prussian blue nanocrystals (PBNCs) and heparin sodium (Hep) through electrospinning, electrospraying, and chemical deposition.					
37268080	4	31	theme	enzyme-like	917:927	arg1	levels					938:943	the enzyme-like activity levels	913:943	the enzyme-like activity levels of PBNCs	913:952	It is worth noting that the nanozymes were firmly anchored to the fiber surfaces through slight polymer swelling caused by the solvent during electrospinning, thereby guaranteeing the preservation of the enzyme-like activity levels of PBNCs.					
37268080	5	32	theme	PPBDH	959:963	arg1	effective					990:998	effective	990:998	effective	990:998	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	5	32	theme	PPBDH	959:963	arg1	dressing					965:972	The PPBDH dressing	955:972	The PPBDH dressing	955:972	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	2	33	theme	composite	313:321	arg1	dressing					335:342	a composite nanofibrous dressing	311:342	a composite nanofibrous dressing	311:342	In this study, a composite nanofibrous dressing was developed by combining Prussian blue nanocrystals (PBNCs) and heparin sodium (Hep) through electrospinning, electrospraying, and chemical deposition.					
37268080	6	34	theme	inflammatory	1346:1357	arg1	response					1359:1366	the inflammatory response	1342:1366	the inflammatory response	1342:1366	Furthermore, a chronic wound healing evaluation conducted in vivo demonstrated that the PPBDH dressing was able to effectively alleviate the inflammatory response and accelerate wound healing.					
37268080	3	35	theme	ROS-scavenging	639:652	arg1	capabilities					654:665	the ROS-scavenging capabilities	635:665	the ROS-scavenging capabilities of PBNCs	635:674	The nanofibrous dressing (PPBDH) was designed to take advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs, resulting in synergistic treatment.					
37268080	4	36	theme	polymer	809:815	arg1	swelling					817:824	slight polymer swelling	802:824	slight polymer swelling caused by the solvent during electrospinning	802:869	It is worth noting that the nanozymes were firmly anchored to the fiber surfaces through slight polymer swelling caused by the solvent during electrospinning, thereby guaranteeing the preservation of the enzyme-like activity levels of PBNCs.					
37268080	4	37	theme	levels	938:943	arg1	preservation					897:908	the preservation	893:908	the preservation of the enzyme-like activity levels of PBNCs	893:952	It is worth noting that the nanozymes were firmly anchored to the fiber surfaces through slight polymer swelling caused by the solvent during electrospinning, thereby guaranteeing the preservation of the enzyme-like activity levels of PBNCs.					
37268080	3	38	theme	Hep	627:629	arg1	capability					613:622	the excellent pro-inflammatory factor-adsorbing capability	565:622	the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs	565:674	The nanofibrous dressing (PPBDH) was designed to take advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs, resulting in synergistic treatment.					
37268080	3	39	theme	excellent	569:577	arg1	capability					613:622	the excellent pro-inflammatory factor-adsorbing capability	565:622	the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs	565:674	The nanofibrous dressing (PPBDH) was designed to take advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs, resulting in synergistic treatment.					
37268080	7	40	theme	wounds	1577:1582	arg1	healing					1543:1549	the healing	1539:1549	the healing of chronic and refractory wounds with uncontrolled inflammation	1539:1613	This research presents an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation.					
37268080	6	41	theme	healing	1234:1240	arg1	evaluation					1242:1251	a chronic wound healing evaluation	1218:1251	a chronic wound healing evaluation conducted in vivo	1218:1269	Furthermore, a chronic wound healing evaluation conducted in vivo demonstrated that the PPBDH dressing was able to effectively alleviate the inflammatory response and accelerate wound healing.					
37268080	2	42	theme	nanofibrous	323:333	arg1	dressing					335:342	a composite nanofibrous dressing	311:342	a composite nanofibrous dressing	311:342	In this study, a composite nanofibrous dressing was developed by combining Prussian blue nanocrystals (PBNCs) and heparin sodium (Hep) through electrospinning, electrospraying, and chemical deposition.					
37268080	0	43	theme	blue	52:55	arg1	Prussian					43:50	Prussian blue	43:55	Prussian blue	43:55	Composite nanofibrous dressing loaded with Prussian blue and heparin for anti-inflammation therapy and diabetic wound healing.					
37268080	3	44	theme	PBNCs	670:674	arg1	Hep					627:629	Hep	627:629	Hep	627:629	The nanofibrous dressing (PPBDH) was designed to take advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs, resulting in synergistic treatment.					
37268080	3	44	theme	PBNCs	670:674	arg1	capabilities					654:665	the ROS-scavenging capabilities	635:665	the ROS-scavenging capabilities of PBNCs	635:674	The nanofibrous dressing (PPBDH) was designed to take advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs, resulting in synergistic treatment.					
37268080	6	45	theme	wound	1228:1232	arg1	healing					1234:1240	a chronic wound healing	1218:1240	a chronic wound healing evaluation conducted in vivo	1218:1269	Furthermore, a chronic wound healing evaluation conducted in vivo demonstrated that the PPBDH dressing was able to effectively alleviate the inflammatory response and accelerate wound healing.					
37268080	1	46	theme	Diabetic	127:134	arg1	ulcer					136:140	Diabetic ulcer	127:140	Diabetic ulcer	127:140	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
37268080	1	46	theme	Diabetic	127:134	arg1	complication					154:165	a severe complication	145:165	a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS)	145:293	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
37268080	5	47	theme	ROS	1026:1028	arg1	levels					1030:1035	intracellular ROS levels	1012:1035	intracellular ROS levels	1012:1035	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	4	48	theme	fiber	779:783	arg1	surfaces					785:792	the fiber surfaces	775:792	the fiber surfaces	775:792	It is worth noting that the nanozymes were firmly anchored to the fiber surfaces through slight polymer swelling caused by the solvent during electrospinning, thereby guaranteeing the preservation of the enzyme-like activity levels of PBNCs.					
37268080	3	49	theme	capability	613:622	arg1	advantage					552:560	advantage	552:560	advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs	552:674	The nanofibrous dressing (PPBDH) was designed to take advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs, resulting in synergistic treatment.					
37268080	4	50	theme	slight	802:807	arg1	swelling					817:824	slight polymer swelling	802:824	slight polymer swelling caused by the solvent during electrospinning	802:869	It is worth noting that the nanozymes were firmly anchored to the fiber surfaces through slight polymer swelling caused by the solvent during electrospinning, thereby guaranteeing the preservation of the enzyme-like activity levels of PBNCs.					
37268080	3	51	theme	capabilities	654:665	arg1	capability					613:622	the excellent pro-inflammatory factor-adsorbing capability	565:622	the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs	565:674	The nanofibrous dressing (PPBDH) was designed to take advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs, resulting in synergistic treatment.					
37268080	7	52	theme	nanofibrous	1473:1483	arg1	dressings					1485:1493	nanozyme hybrid nanofibrous dressings	1457:1493	nanozyme hybrid nanofibrous dressings	1457:1493	This research presents an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation.					
37268080	3	53	theme	nanofibrous	502:512	arg1	PPBDH					524:528	PPBDH	524:528	PPBDH	524:528	The nanofibrous dressing (PPBDH) was designed to take advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs, resulting in synergistic treatment.					
37268080	3	53	theme	nanofibrous	502:512	arg1	dressing					514:521	The nanofibrous dressing	498:521	The nanofibrous dressing (PPBDH)	498:529	The nanofibrous dressing (PPBDH) was designed to take advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs, resulting in synergistic treatment.					
37268080	2	54	theme	chemical	477:484	arg1	deposition					486:495	chemical deposition	477:495	chemical deposition	477:495	In this study, a composite nanofibrous dressing was developed by combining Prussian blue nanocrystals (PBNCs) and heparin sodium (Hep) through electrospinning, electrospraying, and chemical deposition.					
37268080	5	55	theme	ROS-induced	1060:1070	arg1	apoptosis					1072:1080	ROS-induced apoptosis	1060:1080	ROS-induced apoptosis	1060:1080	The PPBDH dressing was found to be effective in reducing intracellular ROS levels, protecting cells from ROS-induced apoptosis, and capturing excessive pro-inflammatory factors, including chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β).					
37268080	3	56	theme	pro-inflammatory	579:594	arg1	capability					613:622	the excellent pro-inflammatory factor-adsorbing capability	565:622	the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs	565:674	The nanofibrous dressing (PPBDH) was designed to take advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs, resulting in synergistic treatment.					
37268080	3	57	theme	synergistic	690:700	arg1	treatment					702:710	synergistic treatment	690:710	synergistic treatment	690:710	The nanofibrous dressing (PPBDH) was designed to take advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs, resulting in synergistic treatment.					
37268080	3	58	theme	factor-adsorbing	596:611	arg1	capability					613:622	the excellent pro-inflammatory factor-adsorbing capability	565:622	the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs	565:674	The nanofibrous dressing (PPBDH) was designed to take advantage of the excellent pro-inflammatory factor-adsorbing capability of Hep and the ROS-scavenging capabilities of PBNCs, resulting in synergistic treatment.					
37268080	7	59	theme	refractory	1566:1575	arg1	wounds					1577:1582	chronic and refractory wounds	1554:1582	chronic and refractory wounds	1554:1582	This research presents an innovative approach to fabricate nanozyme hybrid nanofibrous dressings, which have great potential in accelerating the healing of chronic and refractory wounds with uncontrolled inflammation.					
37268080	1	60	theme	severe	147:152	arg1	ulcer					136:140	Diabetic ulcer	127:140	Diabetic ulcer	127:140	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
37268080	1	60	theme	severe	147:152	arg1	complication					154:165	a severe complication	145:165	a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS)	145:293	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
37268080	1	61	theme	pro-inflammatory	236:251	arg1	factors					253:259	pro-inflammatory factors	236:259	pro-inflammatory factors	236:259	Diabetic ulcer is a severe complication of diabetes that can lead to amputation due to the overproduction of pro-inflammatory factors and reactive oxygen species (ROS).					
35731708	6	0	theme	hydrophilic	1076:1086	arg1	molecules					1092:1100	the hydrophilic CP4 molecules	1072:1100	the hydrophilic CP4 molecules	1072:1100	Our analysis reveals that the hydrophilic CP4 molecules are spontaneously adsorbed onto the membrane outer leaflet surface by virtue of strong electrostatic interactions and do not penetrate into the lipid tail hydrophobic region.					
35731708	9	1	theme	salt	1797:1800	arg1	concentration					1802:1814	a higher salt concentration	1788:1814	a higher salt concentration	1788:1814	The membrane-CP4 interactions are strongly monitored by the ionic strength; a higher salt concentration weakens the binding of CP4 on the membrane surface.					
35731708	7	2	theme	CP4	1301:1303	arg1	binding					1290:1296	This surface binding	1277:1296	This surface binding of CP4	1277:1303	This surface binding of CP4 is strengthened by the formation of anionic lipid-rich domains in their vicinity, causing lateral compositional heterogeneity.					
35731708	2	3	theme	pullulan	447:454	arg1	efficacy					392:399	the excellent broad-spectrum bactericidal efficacy	350:399	the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4)	350:460	In a recent study, the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4) was demonstrated.					
35731708	7	4	theme	lipid-rich	1349:1358	arg1	domains					1360:1366	anionic lipid-rich domains	1341:1366	anionic lipid-rich domains	1341:1366	This surface binding of CP4 is strengthened by the formation of anionic lipid-rich domains in their vicinity, causing lateral compositional heterogeneity.					
35731708	5	5	theme	molecular	1014:1022	arg1	simulations					1033:1043	molecular dynamics simulations	1014:1043	molecular dynamics simulations	1014:1043	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	9	6	theme	CP4	1839:1841	arg1	binding					1828:1834	the binding	1824:1834	the binding of CP4 on the membrane surface	1824:1865	The membrane-CP4 interactions are strongly monitored by the ionic strength; a higher salt concentration weakens the binding of CP4 on the membrane surface.					
35731708	8	7	theme	major	1436:1440	arg1	segregation					1545:1555	(i) anionic lipid segregation	1527:1555	(i) anionic lipid segregation	1527:1555	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	7	theme	major	1436:1440	arg1	outcomes					1442:1449	The major outcomes	1432:1449	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet	1432:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	6	8	theme	membrane	1138:1145	arg1	surface					1161:1167	the membrane outer leaflet surface	1134:1167	the membrane outer leaflet surface	1134:1167	Our analysis reveals that the hydrophilic CP4 molecules are spontaneously adsorbed onto the membrane outer leaflet surface by virtue of strong electrostatic interactions and do not penetrate into the lipid tail hydrophobic region.					
35731708	4	9	theme	polycations	761:771	arg1	activity					749:756	the antimicrobial activity	731:756	the antimicrobial activity of polycations against Gram-positive bacterial membranes	731:813	However, there have been exiguous attempts in the literature to understand the antimicrobial activity of polycations against Gram-positive bacterial membranes.					
35731708	2	10	theme	broad-spectrum	364:377	arg1	efficacy					392:399	the excellent broad-spectrum bactericidal efficacy	350:399	the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4)	350:460	In a recent study, the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4) was demonstrated.					
35731708	5	11	theme	probable	976:983	arg1	function					999:1006	their probable antibacterial function	970:1006	their probable antibacterial function using molecular dynamics simulations	970:1043	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	4	12	theme	Gram-positive	781:793	arg1	membranes					805:813	Gram-positive bacterial membranes	781:813	Gram-positive bacterial membranes	781:813	However, there have been exiguous attempts in the literature to understand the antimicrobial activity of polycations against Gram-positive bacterial membranes.					
35731708	10	13	theme	biomimetic	1961:1970	arg1	POPC					2022:2025	POPC	2022:2025	POPC	2022:2025	In addition, our study also substantiates the non-interacting behavior of CP4 oligomers with biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, indicating their cell selectivity and specificity against pathogenic membranes.					
35731708	10	13	theme	biomimetic	1961:1970	arg1	1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine					1972:2019	biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine	1961:2019	biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane	1961:2035	In addition, our study also substantiates the non-interacting behavior of CP4 oligomers with biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, indicating their cell selectivity and specificity against pathogenic membranes.					
35731708	10	14	theme	oligomers	1946:1954	arg1	behavior					1930:1937	the non-interacting behavior	1910:1937	the non-interacting behavior of CP4 oligomers with biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane	1910:2035	In addition, our study also substantiates the non-interacting behavior of CP4 oligomers with biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, indicating their cell selectivity and specificity against pathogenic membranes.					
35731708	8	15	from	CP4	1504:1506	arg1	leaflet					1515:1521	one leaflet	1511:1521	one leaflet	1511:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	16	theme	lipid	1617:1621	arg1	ordering					1633:1640	the cationic lipid acyl tail ordering	1604:1640	the cationic lipid acyl tail ordering	1604:1640	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	17	theme	polycationic	1491:1502	arg1	CP4					1504:1506	bulky polycationic CP4	1485:1506	bulky polycationic CP4 on one leaflet	1485:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	5	18	theme	first	830:834	arg1	time					836:839	the first time	826:839	the first time	826:839	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	6	19	theme	electrostatic	1189:1201	arg1	interactions					1203:1214	strong electrostatic interactions	1182:1214	strong electrostatic interactions	1182:1214	Our analysis reveals that the hydrophilic CP4 molecules are spontaneously adsorbed onto the membrane outer leaflet surface by virtue of strong electrostatic interactions and do not penetrate into the lipid tail hydrophobic region.					
35731708	8	20	theme	tail	1628:1631	arg1	ordering					1633:1640	the cationic lipid acyl tail ordering	1604:1640	the cationic lipid acyl tail ordering	1604:1640	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	5	21	with	interactions	882:893	arg1	membrane					947:954	a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations	917:1043	a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations	917:1043	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	7	22	theme	compositional	1403:1415	arg1	heterogeneity					1417:1429	lateral compositional heterogeneity	1395:1429	lateral compositional heterogeneity	1395:1429	This surface binding of CP4 is strengthened by the formation of anionic lipid-rich domains in their vicinity, causing lateral compositional heterogeneity.					
35731708	0	23	theme	Ammonium-Substituted	93:112	arg1	Pullulan					127:134	Quaternary Ammonium-Substituted Polycationic Pullulan	82:134	Quaternary Ammonium-Substituted Polycationic Pullulan	82:134	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	0	24	theme	Pullulan	127:134	arg1	Activity					47:54	Anionic Lipid Clustering-Mediated Bactericidal Activity	0:54	Anionic Lipid Clustering-Mediated Bactericidal Activity	0:54	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	0	24	theme	Pullulan	127:134	arg1	Toxicity					70:77	Selective Toxicity	60:77	Selective Toxicity	60:77	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	6	25	theme	leaflet	1153:1159	arg1	surface					1161:1167	the membrane outer leaflet surface	1134:1167	the membrane outer leaflet surface	1134:1167	Our analysis reveals that the hydrophilic CP4 molecules are spontaneously adsorbed onto the membrane outer leaflet surface by virtue of strong electrostatic interactions and do not penetrate into the lipid tail hydrophobic region.					
35731708	8	26	theme	translocation	1660:1672	arg1	ease					1646:1649	ease	1646:1649	ease of water translocation across the lipid hydrophobic barrier	1646:1709	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	26	theme	translocation	1660:1672	arg1	ordering					1633:1640	the cationic lipid acyl tail ordering	1604:1640	the cationic lipid acyl tail ordering	1604:1640	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	5	27	theme	level	876:880	arg1	interactions					882:893	the molecular level interactions	862:893	the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations	862:1043	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	8	28	from	leaflet	1515:1521	arg1	accumulation					1469:1480	the asymmetric accumulation	1454:1480	the asymmetric accumulation of bulky polycationic CP4 on one leaflet	1454:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	6	29	theme	lipid	1246:1250	arg1	region					1269:1274	the lipid tail hydrophobic region	1242:1274	the lipid tail hydrophobic region	1242:1274	Our analysis reveals that the hydrophilic CP4 molecules are spontaneously adsorbed onto the membrane outer leaflet surface by virtue of strong electrostatic interactions and do not penetrate into the lipid tail hydrophobic region.					
35731708	0	30	theme	Quaternary	82:91	arg1	Pullulan					127:134	Quaternary Ammonium-Substituted Polycationic Pullulan	82:134	Quaternary Ammonium-Substituted Polycationic Pullulan	82:134	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	8	31	theme	lipid	1539:1543	arg1	segregation					1545:1555	(i) anionic lipid segregation	1527:1555	(i) anionic lipid segregation	1527:1555	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	31	theme	lipid	1539:1543	arg1	outcomes					1442:1449	The major outcomes	1432:1449	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet	1432:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	0	32	theme	Anionic	0:6	arg1	Activity					47:54	Anionic Lipid Clustering-Mediated Bactericidal Activity	0:54	Anionic Lipid Clustering-Mediated Bactericidal Activity	0:54	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	8	33	theme	hydrophobic	1691:1701	arg1	barrier					1703:1709	the lipid hydrophobic barrier	1681:1709	the lipid hydrophobic barrier	1681:1709	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	3	34	theme	resistance	558:567	arg1	acquisition					543:553	the acquisition	539:553	the acquisition of resistance among pathogens	539:583	Their selective toxicity and nominal probability to induce the acquisition of resistance among pathogens fulfill the fundamental requirements of new-generation antibacterials.					
35731708	0	35	theme	Clustering-Mediated	14:32	arg1	Activity					47:54	Anionic Lipid Clustering-Mediated Bactericidal Activity	0:54	Anionic Lipid Clustering-Mediated Bactericidal Activity	0:54	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	8	36	theme	interaction	1564:1574	arg1	site					1576:1579	the interaction site	1560:1579	the interaction site	1560:1579	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	3	37	theme	selective	486:494	arg1	toxicity					496:503	Their selective toxicity	480:503	Their selective toxicity	480:503	Their selective toxicity and nominal probability to induce the acquisition of resistance among pathogens fulfill the fundamental requirements of new-generation antibacterials.					
35731708	2	38	theme	cationic	438:445	arg1	CP4					457:459	CP4	457:459	CP4	457:459	In a recent study, the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4) was demonstrated.					
35731708	2	38	theme	cationic	438:445	arg1	pullulan					447:454	a quaternary ammonium-substituted cationic pullulan	404:454	a quaternary ammonium-substituted cationic pullulan (CP4)	404:460	In a recent study, the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4) was demonstrated.					
35731708	5	39	theme	Staphylococcus	925:938	arg1	membrane					947:954	a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations	917:1043	a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations	917:1043	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	9	40	theme	ionic	1772:1776	arg1	strength					1778:1785	the ionic strength	1768:1785	the ionic strength	1768:1785	The membrane-CP4 interactions are strongly monitored by the ionic strength; a higher salt concentration weakens the binding of CP4 on the membrane surface.					
35731708	2	41	theme	quaternary	406:415	arg1	CP4					457:459	CP4	457:459	CP4	457:459	In a recent study, the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4) was demonstrated.					
35731708	2	41	theme	quaternary	406:415	arg1	pullulan					447:454	a quaternary ammonium-substituted cationic pullulan	404:454	a quaternary ammonium-substituted cationic pullulan (CP4)	404:460	In a recent study, the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4) was demonstrated.					
35731708	1	42	theme	great	295:299	arg1	compatibility					316:328	great mammalian cell compatibility	295:328	great mammalian cell compatibility	295:328	Non-amphiphilic polycations have recently been recognized to hold excellent antimicrobial potential with great mammalian cell compatibility.					
35731708	3	43	theme	new-generation	625:638	arg1	antibacterials					640:653	new-generation antibacterials	625:653	new-generation antibacterials	625:653	Their selective toxicity and nominal probability to induce the acquisition of resistance among pathogens fulfill the fundamental requirements of new-generation antibacterials.					
35731708	8	44	dep	segregation	1545:1555	arg1	i					1528:1528	i	1528:1528	i	1528:1528	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	1	45	theme	cell	311:314	arg1	compatibility					316:328	great mammalian cell compatibility	295:328	great mammalian cell compatibility	295:328	Non-amphiphilic polycations have recently been recognized to hold excellent antimicrobial potential with great mammalian cell compatibility.					
35731708	6	46	theme	CP4	1088:1090	arg1	molecules					1092:1100	the hydrophilic CP4 molecules	1072:1100	the hydrophilic CP4 molecules	1072:1100	Our analysis reveals that the hydrophilic CP4 molecules are spontaneously adsorbed onto the membrane outer leaflet surface by virtue of strong electrostatic interactions and do not penetrate into the lipid tail hydrophobic region.					
35731708	9	47	theme	higher	1790:1795	arg1	concentration					1802:1814	a higher salt concentration	1788:1814	a higher salt concentration	1788:1814	The membrane-CP4 interactions are strongly monitored by the ionic strength; a higher salt concentration weakens the binding of CP4 on the membrane surface.					
35731708	4	48	theme	antimicrobial	735:747	arg1	activity					749:756	the antimicrobial activity	731:756	the antimicrobial activity of polycations against Gram-positive bacterial membranes	731:813	However, there have been exiguous attempts in the literature to understand the antimicrobial activity of polycations against Gram-positive bacterial membranes.					
35731708	5	49	theme	CP4	898:900	arg1	tetramers					902:910	CP4 tetramers	898:910	CP4 tetramers	898:910	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	6	50	theme	hydrophobic	1257:1267	arg1	region					1269:1274	the lipid tail hydrophobic region	1242:1274	the lipid tail hydrophobic region	1242:1274	Our analysis reveals that the hydrophilic CP4 molecules are spontaneously adsorbed onto the membrane outer leaflet surface by virtue of strong electrostatic interactions and do not penetrate into the lipid tail hydrophobic region.					
35731708	10	51	theme	pathogenic	2096:2105	arg1	membranes					2107:2115	pathogenic membranes	2096:2115	pathogenic membranes	2096:2115	In addition, our study also substantiates the non-interacting behavior of CP4 oligomers with biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, indicating their cell selectivity and specificity against pathogenic membranes.					
35731708	7	52	theme	surface	1282:1288	arg1	binding					1290:1296	This surface binding	1277:1296	This surface binding of CP4	1277:1303	This surface binding of CP4 is strengthened by the formation of anionic lipid-rich domains in their vicinity, causing lateral compositional heterogeneity.					
35731708	2	53	theme	excellent	354:362	arg1	efficacy					392:399	the excellent broad-spectrum bactericidal efficacy	350:399	the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4)	350:460	In a recent study, the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4) was demonstrated.					
35731708	8	54	from	site	1576:1579	arg1	segregation					1545:1555	(i) anionic lipid segregation	1527:1555	(i) anionic lipid segregation	1527:1555	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	54	from	site	1576:1579	arg1	ease					1646:1649	ease	1646:1649	ease of water translocation across the lipid hydrophobic barrier	1646:1709	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	54	from	site	1576:1579	arg1	outcomes					1442:1449	The major outcomes	1432:1449	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet	1432:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	54	from	site	1576:1579	arg1	ordering					1633:1640	the cationic lipid acyl tail ordering	1604:1640	the cationic lipid acyl tail ordering	1604:1640	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	0	55	theme	Selective	60:68	arg1	Toxicity					70:77	Selective Toxicity	60:77	Selective Toxicity	60:77	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	5	56	dep	Staphylococcus	925:938	arg1	aureus					940:945	aureus	940:945	aureus	940:945	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	4	57	theme	bacterial	795:803	arg1	membranes					805:813	Gram-positive bacterial membranes	781:813	Gram-positive bacterial membranes	781:813	However, there have been exiguous attempts in the literature to understand the antimicrobial activity of polycations against Gram-positive bacterial membranes.					
35731708	5	58	theme	tetramers	902:910	arg1	interactions					882:893	the molecular level interactions	862:893	the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations	862:1043	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	10	59	theme	CP4	1942:1944	arg1	oligomers					1946:1954	CP4 oligomers	1942:1954	CP4 oligomers	1942:1954	In addition, our study also substantiates the non-interacting behavior of CP4 oligomers with biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, indicating their cell selectivity and specificity against pathogenic membranes.					
35731708	5	60	theme	dynamics	1024:1031	arg1	simulations					1033:1043	molecular dynamics simulations	1014:1043	molecular dynamics simulations	1014:1043	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	6	61	theme	outer	1147:1151	arg1	surface					1161:1167	the membrane outer leaflet surface	1134:1167	the membrane outer leaflet surface	1134:1167	Our analysis reveals that the hydrophilic CP4 molecules are spontaneously adsorbed onto the membrane outer leaflet surface by virtue of strong electrostatic interactions and do not penetrate into the lipid tail hydrophobic region.					
35731708	0	62	theme	Bacterial	170:178	arg1	Membrane					180:187	the Staphylococcus aureus Bacterial Membrane	144:187	the Staphylococcus aureus Bacterial Membrane	144:187	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	2	63	theme	bactericidal	379:390	arg1	efficacy					392:399	the excellent broad-spectrum bactericidal efficacy	350:399	the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4)	350:460	In a recent study, the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4) was demonstrated.					
35731708	1	64	theme	excellent	256:264	arg1	potential					280:288	excellent antimicrobial potential	256:288	excellent antimicrobial potential	256:288	Non-amphiphilic polycations have recently been recognized to hold excellent antimicrobial potential with great mammalian cell compatibility.					
35731708	9	65	from	binding	1828:1834	arg1	surface					1859:1865	the membrane surface	1846:1865	the membrane surface	1846:1865	The membrane-CP4 interactions are strongly monitored by the ionic strength; a higher salt concentration weakens the binding of CP4 on the membrane surface.					
35731708	9	66	theme	membrane	1850:1857	arg1	surface					1859:1865	the membrane surface	1846:1865	the membrane surface	1846:1865	The membrane-CP4 interactions are strongly monitored by the ionic strength; a higher salt concentration weakens the binding of CP4 on the membrane surface.					
35731708	2	67	theme	ammonium-substituted	417:436	arg1	CP4					457:459	CP4	457:459	CP4	457:459	In a recent study, the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4) was demonstrated.					
35731708	2	67	theme	ammonium-substituted	417:436	arg1	pullulan					447:454	a quaternary ammonium-substituted cationic pullulan	404:454	a quaternary ammonium-substituted cationic pullulan (CP4)	404:460	In a recent study, the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4) was demonstrated.					
35731708	10	68	theme	1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine	1972:2019	arg1	membrane					2028:2035	biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane	1961:2035	biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane	1961:2035	In addition, our study also substantiates the non-interacting behavior of CP4 oligomers with biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, indicating their cell selectivity and specificity against pathogenic membranes.					
35731708	5	69	theme	antibacterial	985:997	arg1	function					999:1006	their probable antibacterial function	970:1006	their probable antibacterial function using molecular dynamics simulations	970:1043	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	8	70	theme	cationic	1608:1615	arg1	ordering					1633:1640	the cationic lipid acyl tail ordering	1604:1640	the cationic lipid acyl tail ordering	1604:1640	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	71	dep	decrease	1592:1599	arg1	ii					1586:1587	ii	1586:1587	ii	1586:1587	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	72	theme	acyl	1623:1626	arg1	ordering					1633:1640	the cationic lipid acyl tail ordering	1604:1640	the cationic lipid acyl tail ordering	1604:1640	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	73	theme	accumulation	1469:1480	arg1	segregation					1545:1555	(i) anionic lipid segregation	1527:1555	(i) anionic lipid segregation	1527:1555	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	73	theme	accumulation	1469:1480	arg1	outcomes					1442:1449	The major outcomes	1432:1449	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet	1432:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	2	74	theme	recent	336:341	arg1	study					343:347	a recent study	334:347	a recent study	334:347	In a recent study, the excellent broad-spectrum bactericidal efficacy of a quaternary ammonium-substituted cationic pullulan (CP4) was demonstrated.					
35731708	6	75	theme	interactions	1203:1214	arg1	virtue					1172:1177	virtue	1172:1177	virtue of strong electrostatic interactions	1172:1214	Our analysis reveals that the hydrophilic CP4 molecules are spontaneously adsorbed onto the membrane outer leaflet surface by virtue of strong electrostatic interactions and do not penetrate into the lipid tail hydrophobic region.					
35731708	8	76	theme	bulky	1485:1489	arg1	CP4					1504:1506	bulky polycationic CP4	1485:1506	bulky polycationic CP4 on one leaflet	1485:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	7	77	theme	lateral	1395:1401	arg1	heterogeneity					1417:1429	lateral compositional heterogeneity	1395:1429	lateral compositional heterogeneity	1395:1429	This surface binding of CP4 is strengthened by the formation of anionic lipid-rich domains in their vicinity, causing lateral compositional heterogeneity.					
35731708	1	78	theme	Non-amphiphilic	190:204	arg1	polycations					206:216	Non-amphiphilic polycations	190:216	Non-amphiphilic polycations	190:216	Non-amphiphilic polycations have recently been recognized to hold excellent antimicrobial potential with great mammalian cell compatibility.					
35731708	6	79	theme	strong	1182:1187	arg1	interactions					1203:1214	strong electrostatic interactions	1182:1214	strong electrostatic interactions	1182:1214	Our analysis reveals that the hydrophilic CP4 molecules are spontaneously adsorbed onto the membrane outer leaflet surface by virtue of strong electrostatic interactions and do not penetrate into the lipid tail hydrophobic region.					
35731708	8	80	theme	CP4	1504:1506	arg1	accumulation					1469:1480	the asymmetric accumulation	1454:1480	the asymmetric accumulation of bulky polycationic CP4 on one leaflet	1454:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	0	81	theme	Polycationic	114:125	arg1	Pullulan					127:134	Quaternary Ammonium-Substituted Polycationic Pullulan	82:134	Quaternary Ammonium-Substituted Polycationic Pullulan	82:134	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	10	82	with	behavior	1930:1937	arg1	membrane					2028:2035	biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane	1961:2035	biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane	1961:2035	In addition, our study also substantiates the non-interacting behavior of CP4 oligomers with biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, indicating their cell selectivity and specificity against pathogenic membranes.					
35731708	8	83	theme	water	1654:1658	arg1	translocation					1660:1672	water translocation	1654:1672	water translocation across the lipid hydrophobic barrier	1654:1709	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	0	84	dep	Staphylococcus	148:161	arg1	aureus					163:168	aureus	163:168	aureus	163:168	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	10	85	theme	non-interacting	1914:1928	arg1	behavior					1930:1937	the non-interacting behavior	1910:1937	the non-interacting behavior of CP4 oligomers with biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane	1910:2035	In addition, our study also substantiates the non-interacting behavior of CP4 oligomers with biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, indicating their cell selectivity and specificity against pathogenic membranes.					
35731708	7	86	from	formation	1328:1336	arg1	vicinity					1377:1384	their vicinity	1371:1384	their vicinity	1371:1384	This surface binding of CP4 is strengthened by the formation of anionic lipid-rich domains in their vicinity, causing lateral compositional heterogeneity.					
35731708	0	87	theme	Staphylococcus	148:161	arg1	Membrane					180:187	the Staphylococcus aureus Bacterial Membrane	144:187	the Staphylococcus aureus Bacterial Membrane	144:187	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	1	88	theme	antimicrobial	266:278	arg1	potential					280:288	excellent antimicrobial potential	256:288	excellent antimicrobial potential	256:288	Non-amphiphilic polycations have recently been recognized to hold excellent antimicrobial potential with great mammalian cell compatibility.					
35731708	6	89	theme	tail	1252:1255	arg1	region					1269:1274	the lipid tail hydrophobic region	1242:1274	the lipid tail hydrophobic region	1242:1274	Our analysis reveals that the hydrophilic CP4 molecules are spontaneously adsorbed onto the membrane outer leaflet surface by virtue of strong electrostatic interactions and do not penetrate into the lipid tail hydrophobic region.					
35731708	8	90	theme	lipid	1685:1689	arg1	barrier					1703:1709	the lipid hydrophobic barrier	1681:1709	the lipid hydrophobic barrier	1681:1709	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	3	91	dep	toxicity	496:503	arg1	induce					532:537	induce	532:537	to induce the acquisition of resistance among pathogens	529:583	Their selective toxicity and nominal probability to induce the acquisition of resistance among pathogens fulfill the fundamental requirements of new-generation antibacterials.					
35731708	7	92	theme	domains	1360:1366	arg1	formation					1328:1336	the formation	1324:1336	the formation of anionic lipid-rich domains in their vicinity	1324:1384	This surface binding of CP4 is strengthened by the formation of anionic lipid-rich domains in their vicinity, causing lateral compositional heterogeneity.					
35731708	0	93	theme	Lipid	8:12	arg1	Activity					47:54	Anionic Lipid Clustering-Mediated Bactericidal Activity	0:54	Anionic Lipid Clustering-Mediated Bactericidal Activity	0:54	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	9	94	theme	membrane-CP4	1716:1727	arg1	interactions					1729:1740	The membrane-CP4 interactions	1712:1740	The membrane-CP4 interactions	1712:1740	The membrane-CP4 interactions are strongly monitored by the ionic strength; a higher salt concentration weakens the binding of CP4 on the membrane surface.					
35731708	8	95	theme	anionic	1531:1537	arg1	segregation					1545:1555	(i) anionic lipid segregation	1527:1555	(i) anionic lipid segregation	1527:1555	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	95	theme	anionic	1531:1537	arg1	outcomes					1442:1449	The major outcomes	1432:1449	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet	1432:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	0	96	theme	Bactericidal	34:45	arg1	Activity					47:54	Anionic Lipid Clustering-Mediated Bactericidal Activity	0:54	Anionic Lipid Clustering-Mediated Bactericidal Activity	0:54	Anionic Lipid Clustering-Mediated Bactericidal Activity and Selective Toxicity of Quaternary Ammonium-Substituted Polycationic Pullulan against the Staphylococcus aureus Bacterial Membrane.					
35731708	8	97	from	accumulation	1469:1480	arg1	leaflet					1515:1521	one leaflet	1511:1521	one leaflet	1511:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	10	98	theme	cell	2055:2058	arg1	selectivity					2060:2070	their cell selectivity	2049:2070	their cell selectivity	2049:2070	In addition, our study also substantiates the non-interacting behavior of CP4 oligomers with biomimetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, indicating their cell selectivity and specificity against pathogenic membranes.					
35731708	8	99	theme	asymmetric	1458:1467	arg1	accumulation					1469:1480	the asymmetric accumulation	1454:1480	the asymmetric accumulation of bulky polycationic CP4 on one leaflet	1454:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	3	100	theme	nominal	509:515	arg1	probability					517:527	nominal probability	509:527	nominal probability	509:527	Their selective toxicity and nominal probability to induce the acquisition of resistance among pathogens fulfill the fundamental requirements of new-generation antibacterials.					
35731708	5	101	theme	model	919:923	arg1	membrane					947:954	a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations	917:1043	a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations	917:1043	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	3	102	theme	fundamental	597:607	arg1	requirements					609:620	the fundamental requirements	593:620	the fundamental requirements of new-generation antibacterials	593:653	Their selective toxicity and nominal probability to induce the acquisition of resistance among pathogens fulfill the fundamental requirements of new-generation antibacterials.					
35731708	8	103	from	decrease	1592:1599	arg1	segregation					1545:1555	(i) anionic lipid segregation	1527:1555	(i) anionic lipid segregation	1527:1555	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	103	from	decrease	1592:1599	arg1	ease					1646:1649	ease	1646:1649	ease of water translocation across the lipid hydrophobic barrier	1646:1709	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	103	from	decrease	1592:1599	arg1	outcomes					1442:1449	The major outcomes	1432:1449	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet	1432:1521	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	8	103	from	decrease	1592:1599	arg1	ordering					1633:1640	the cationic lipid acyl tail ordering	1604:1640	the cationic lipid acyl tail ordering	1604:1640	The major outcomes of the asymmetric accumulation of bulky polycationic CP4 on one leaflet are (i) anionic lipid segregation at the interaction site and (ii) a decrease in the cationic lipid acyl tail ordering and ease of water translocation across the lipid hydrophobic barrier.					
35731708	5	104	theme	molecular	866:874	arg1	interactions					882:893	the molecular level interactions	862:893	the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations	862:1043	Here, for the first time, we have scrutinized the molecular level interactions of CP4 tetramers with a model Staphylococcus aureus membrane to understand their probable antibacterial function using molecular dynamics simulations.					
35731708	3	105	theme	antibacterials	640:653	arg1	requirements					609:620	the fundamental requirements	593:620	the fundamental requirements of new-generation antibacterials	593:653	Their selective toxicity and nominal probability to induce the acquisition of resistance among pathogens fulfill the fundamental requirements of new-generation antibacterials.					
35731708	1	106	theme	mammalian	301:309	arg1	compatibility					316:328	great mammalian cell compatibility	295:328	great mammalian cell compatibility	295:328	Non-amphiphilic polycations have recently been recognized to hold excellent antimicrobial potential with great mammalian cell compatibility.					
35731708	7	107	theme	anionic	1341:1347	arg1	domains					1360:1366	anionic lipid-rich domains	1341:1366	anionic lipid-rich domains	1341:1366	This surface binding of CP4 is strengthened by the formation of anionic lipid-rich domains in their vicinity, causing lateral compositional heterogeneity.					
36155783	5	0	theme	hydrogel	827:834	arg1	systems					836:842	the hydrogel systems	823:842	the hydrogel systems	823:842	Finally, human hair samples have been treated with the hydrogel systems by the dipping procedure.					
36155783	6	1	theme	fibers	975:980	arg1	properties					956:965	the tensile properties	944:965	the tensile properties of hair fibers exposed to UV irradiation	944:1006	The protection efficiency of the hydrogels has been evaluated by studying the tensile properties of hair fibers exposed to UV irradiation.					
36155783	7	2	theme	keratin/alginate	1024:1039	arg1	hydrogel					1041:1048	keratin/alginate hydrogel	1024:1048	keratin/alginate hydrogel filled with halloysite	1024:1071	In conclusion, keratin/alginate hydrogel filled with halloysite represents a promising formulation for hair protective treatments due to the peculiar structural and rheological characteristics.					
36155783	2	3	theme	Ca2+	361:364	arg1	ions					366:369	Ca2+ ions	361:369	Ca2+ ions	361:369	Preliminary studies have been conducted on the aqueous colloidal systems and the corresponding hydrogels obtained by using Ca2+ ions as crosslinkers.					
36155783	2	3	theme	Ca2+	361:364	arg1	crosslinkers					374:385	crosslinkers	374:385	crosslinkers	374:385	Preliminary studies have been conducted on the aqueous colloidal systems and the corresponding hydrogels obtained by using Ca2+ ions as crosslinkers.					
36155783	4	4	theme	hydrogels	616:624	arg1	properties					598:607	the rheological properties	582:607	the rheological properties of the hydrogels	582:624	Then, the rheological properties of the hydrogels have been studied highlighting that the keratin/alginate interactions and the subsequent addition of HNTs facilitate the biopolymer crosslinking.					
36155783	0	5	theme	hair	106:109	arg1	treatment					87:95	protective treatment	76:95	protective treatment of human hair	76:109	Keratin/alginate hybrid hydrogels filled with halloysite clay nanotubes for protective treatment of human hair.					
36155783	6	6	theme	hair	970:973	arg1	fibers					975:980	hair fibers	970:980	hair fibers exposed to UV irradiation	970:1006	The protection efficiency of the hydrogels has been evaluated by studying the tensile properties of hair fibers exposed to UV irradiation.					
36155783	6	7	theme	protection	874:883	arg1	efficiency					885:894	The protection efficiency	870:894	The protection efficiency of the hydrogels	870:911	The protection efficiency of the hydrogels has been evaluated by studying the tensile properties of hair fibers exposed to UV irradiation.					
36155783	2	8	theme	colloidal	293:301	arg1	systems					303:309	the aqueous colloidal systems	281:309	the aqueous colloidal systems	281:309	Preliminary studies have been conducted on the aqueous colloidal systems and the corresponding hydrogels obtained by using Ca2+ ions as crosslinkers.					
36155783	0	9	theme	human	100:104	arg1	hair					106:109	human hair	100:109	human hair	100:109	Keratin/alginate hybrid hydrogels filled with halloysite clay nanotubes for protective treatment of human hair.					
36155783	2	10	theme	aqueous	285:291	arg1	systems					303:309	the aqueous colloidal systems	281:309	the aqueous colloidal systems	281:309	Preliminary studies have been conducted on the aqueous colloidal systems and the corresponding hydrogels obtained by using Ca2+ ions as crosslinkers.					
36155783	3	11	theme	specific	495:502	arg1	interactions					504:515	the specific interactions	491:515	the specific interactions occurring between the biomacromolecules and the nanotubes	491:573	Firstly, we have investigated the colloidal properties of keratin/alginate/HNTs dispersions to explore the specific interactions occurring between the biomacromolecules and the nanotubes.					
36155783	3	12	theme	keratin/alginate/HNTs	446:466	arg1	dispersions					468:478	keratin/alginate/HNTs dispersions	446:478	keratin/alginate/HNTs dispersions	446:478	Firstly, we have investigated the colloidal properties of keratin/alginate/HNTs dispersions to explore the specific interactions occurring between the biomacromolecules and the nanotubes.					
36155783	6	13	theme	tensile	948:954	arg1	properties					956:965	the tensile properties	944:965	the tensile properties of hair fibers exposed to UV irradiation	944:1006	The protection efficiency of the hydrogels has been evaluated by studying the tensile properties of hair fibers exposed to UV irradiation.					
36155783	3	14	theme	dispersions	468:478	arg1	properties					432:441	the colloidal properties	418:441	the colloidal properties of keratin/alginate/HNTs dispersions	418:478	Firstly, we have investigated the colloidal properties of keratin/alginate/HNTs dispersions to explore the specific interactions occurring between the biomacromolecules and the nanotubes.					
36155783	0	15	theme	hybrid	17:22	arg1	hydrogels					24:32	Keratin/alginate hybrid hydrogels	0:32	Keratin/alginate hybrid hydrogels	0:32	Keratin/alginate hybrid hydrogels filled with halloysite clay nanotubes for protective treatment of human hair.					
36155783	2	16	theme	corresponding	319:331	arg1	hydrogels					333:341	the corresponding hydrogels	315:341	the corresponding hydrogels obtained by using Ca2+ ions as crosslinkers	315:385	Preliminary studies have been conducted on the aqueous colloidal systems and the corresponding hydrogels obtained by using Ca2+ ions as crosslinkers.					
36155783	0	17	theme	Keratin/alginate	0:15	arg1	hydrogels					24:32	Keratin/alginate hybrid hydrogels	0:32	Keratin/alginate hybrid hydrogels	0:32	Keratin/alginate hybrid hydrogels filled with halloysite clay nanotubes for protective treatment of human hair.					
36155783	7	18	theme	rheological	1174:1184	arg1	characteristics					1186:1200	the peculiar structural and rheological characteristics	1146:1200	the peculiar structural and rheological characteristics	1146:1200	In conclusion, keratin/alginate hydrogel filled with halloysite represents a promising formulation for hair protective treatments due to the peculiar structural and rheological characteristics.					
36155783	4	19	theme	HNTs	727:730	arg1	interactions					683:694	the keratin/alginate interactions	662:694	the keratin/alginate interactions	662:694	Then, the rheological properties of the hydrogels have been studied highlighting that the keratin/alginate interactions and the subsequent addition of HNTs facilitate the biopolymer crosslinking.					
36155783	4	19	theme	HNTs	727:730	arg1	addition					715:722	the subsequent addition	700:722	the subsequent addition of HNTs	700:730	Then, the rheological properties of the hydrogels have been studied highlighting that the keratin/alginate interactions and the subsequent addition of HNTs facilitate the biopolymer crosslinking.					
36155783	4	20	theme	rheological	586:596	arg1	properties					598:607	the rheological properties	582:607	the rheological properties of the hydrogels	582:624	Then, the rheological properties of the hydrogels have been studied highlighting that the keratin/alginate interactions and the subsequent addition of HNTs facilitate the biopolymer crosslinking.					
36155783	7	21	theme	peculiar	1150:1157	arg1	characteristics					1186:1200	the peculiar structural and rheological characteristics	1146:1200	the peculiar structural and rheological characteristics	1146:1200	In conclusion, keratin/alginate hydrogel filled with halloysite represents a promising formulation for hair protective treatments due to the peculiar structural and rheological characteristics.					
36155783	7	22	theme	structural	1159:1168	arg1	characteristics					1186:1200	the peculiar structural and rheological characteristics	1146:1200	the peculiar structural and rheological characteristics	1146:1200	In conclusion, keratin/alginate hydrogel filled with halloysite represents a promising formulation for hair protective treatments due to the peculiar structural and rheological characteristics.					
36155783	1	23	theme	Keratin/alginate	112:127	arg1	hydrogels					129:137	Keratin/alginate hydrogels	112:137	Keratin/alginate hydrogels filled with halloysite nanotubes (HNTs)	112:177	Keratin/alginate hydrogels filled with halloysite nanotubes (HNTs) have been tested for the protective coating of human hair.					
36155783	1	24	theme	protective	204:213	arg1	coating					215:221	the protective coating	200:221	the protective coating of human hair	200:235	Keratin/alginate hydrogels filled with halloysite nanotubes (HNTs) have been tested for the protective coating of human hair.					
36155783	0	25	theme	halloysite	46:55	arg1	nanotubes					62:70	halloysite clay nanotubes	46:70	halloysite clay nanotubes for protective treatment of human hair	46:109	Keratin/alginate hybrid hydrogels filled with halloysite clay nanotubes for protective treatment of human hair.					
36155783	5	26	theme	human	781:785	arg1	samples					792:798	human hair samples	781:798	human hair samples	781:798	Finally, human hair samples have been treated with the hydrogel systems by the dipping procedure.					
36155783	2	27	theme	Preliminary	238:248	arg1	studies					250:256	Preliminary studies	238:256	Preliminary studies	238:256	Preliminary studies have been conducted on the aqueous colloidal systems and the corresponding hydrogels obtained by using Ca2+ ions as crosslinkers.					
36155783	4	28	theme	subsequent	704:713	arg1	addition					715:722	the subsequent addition	700:722	the subsequent addition of HNTs	700:730	Then, the rheological properties of the hydrogels have been studied highlighting that the keratin/alginate interactions and the subsequent addition of HNTs facilitate the biopolymer crosslinking.					
36155783	3	29	theme	colloidal	422:430	arg1	properties					432:441	the colloidal properties	418:441	the colloidal properties of keratin/alginate/HNTs dispersions	418:478	Firstly, we have investigated the colloidal properties of keratin/alginate/HNTs dispersions to explore the specific interactions occurring between the biomacromolecules and the nanotubes.					
36155783	5	30	theme	hair	787:790	arg1	samples					792:798	human hair samples	781:798	human hair samples	781:798	Finally, human hair samples have been treated with the hydrogel systems by the dipping procedure.					
36155783	5	31	theme	dipping	851:857	arg1	procedure					859:867	the dipping procedure	847:867	the dipping procedure	847:867	Finally, human hair samples have been treated with the hydrogel systems by the dipping procedure.					
36155783	0	32	theme	clay	57:60	arg1	nanotubes					62:70	halloysite clay nanotubes	46:70	halloysite clay nanotubes for protective treatment of human hair	46:109	Keratin/alginate hybrid hydrogels filled with halloysite clay nanotubes for protective treatment of human hair.					
36155783	7	33	theme	due	1139:1141	arg1	treatments					1128:1137	hair protective treatments	1112:1137	hair protective treatments due to the peculiar structural and rheological characteristics	1112:1200	In conclusion, keratin/alginate hydrogel filled with halloysite represents a promising formulation for hair protective treatments due to the peculiar structural and rheological characteristics.					
36155783	6	34	theme	UV	993:994	arg1	irradiation					996:1006	UV irradiation	993:1006	UV irradiation	993:1006	The protection efficiency of the hydrogels has been evaluated by studying the tensile properties of hair fibers exposed to UV irradiation.					
36155783	7	35	theme	hair	1112:1115	arg1	treatments					1128:1137	hair protective treatments	1112:1137	hair protective treatments due to the peculiar structural and rheological characteristics	1112:1200	In conclusion, keratin/alginate hydrogel filled with halloysite represents a promising formulation for hair protective treatments due to the peculiar structural and rheological characteristics.					
36155783	6	36	theme	hydrogels	903:911	arg1	efficiency					885:894	The protection efficiency	870:894	The protection efficiency of the hydrogels	870:911	The protection efficiency of the hydrogels has been evaluated by studying the tensile properties of hair fibers exposed to UV irradiation.					
36155783	7	37	theme	promising	1086:1094	arg1	formulation					1096:1106	a promising formulation	1084:1106	a promising formulation for hair protective treatments due to the peculiar structural and rheological characteristics	1084:1200	In conclusion, keratin/alginate hydrogel filled with halloysite represents a promising formulation for hair protective treatments due to the peculiar structural and rheological characteristics.					
36155783	1	38	theme	human	226:230	arg1	hair					232:235	human hair	226:235	human hair	226:235	Keratin/alginate hydrogels filled with halloysite nanotubes (HNTs) have been tested for the protective coating of human hair.					
36155783	7	39	theme	protective	1117:1126	arg1	treatments					1128:1137	hair protective treatments	1112:1137	hair protective treatments due to the peculiar structural and rheological characteristics	1112:1200	In conclusion, keratin/alginate hydrogel filled with halloysite represents a promising formulation for hair protective treatments due to the peculiar structural and rheological characteristics.					
36155783	1	40	theme	halloysite	151:160	arg1	HNTs					173:176	HNTs	173:176	HNTs	173:176	Keratin/alginate hydrogels filled with halloysite nanotubes (HNTs) have been tested for the protective coating of human hair.					
36155783	1	40	theme	halloysite	151:160	arg1	nanotubes					162:170	halloysite nanotubes	151:170	halloysite nanotubes (HNTs)	151:177	Keratin/alginate hydrogels filled with halloysite nanotubes (HNTs) have been tested for the protective coating of human hair.					
36155783	1	41	theme	hair	232:235	arg1	coating					215:221	the protective coating	200:221	the protective coating of human hair	200:235	Keratin/alginate hydrogels filled with halloysite nanotubes (HNTs) have been tested for the protective coating of human hair.					
36155783	0	42	theme	protective	76:85	arg1	treatment					87:95	protective treatment	76:95	protective treatment of human hair	76:109	Keratin/alginate hybrid hydrogels filled with halloysite clay nanotubes for protective treatment of human hair.					
36155783	4	43	theme	biopolymer	747:756	arg1	crosslinking					758:769	the biopolymer crosslinking	743:769	the biopolymer crosslinking	743:769	Then, the rheological properties of the hydrogels have been studied highlighting that the keratin/alginate interactions and the subsequent addition of HNTs facilitate the biopolymer crosslinking.					
36155783	4	44	theme	keratin/alginate	666:681	arg1	interactions					683:694	the keratin/alginate interactions	662:694	the keratin/alginate interactions	662:694	Then, the rheological properties of the hydrogels have been studied highlighting that the keratin/alginate interactions and the subsequent addition of HNTs facilitate the biopolymer crosslinking.					
35797143	3	0	theme	dispersive	662:671	arg1	spectroscopy					679:690	energy dispersive X-ray spectroscopy	655:690	energy dispersive X-ray spectroscopy	655:690	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	10	1	theme	toxic	1681:1685	arg1	effects					1687:1693	the toxic effects	1677:1693	the toxic effects of ZnO nanoparticles	1677:1714	The HSP increased the antioxidant capacity of both the ZnO-HSP particles and the CS/ZnO-HSP nanocomposites, reducing the toxic effects of ZnO nanoparticles.					
35797143	10	2	theme	nanoparticles	1702:1714	arg1	effects					1687:1693	the toxic effects	1677:1693	the toxic effects of ZnO nanoparticles	1677:1714	The HSP increased the antioxidant capacity of both the ZnO-HSP particles and the CS/ZnO-HSP nanocomposites, reducing the toxic effects of ZnO nanoparticles.					
35797143	6	3	theme	6 wt	1127:1130	arg1	%					1131:1131	6 wt%	1127:1131	6 wt%	1127:1131	While the <i>T</i><sub>g</sub> value of CS was 81 °C, this value increased by 13-94 °C with the addition of 6 wt% by weight of the ZnO-HSP.					
35797143	1	4	theme	strong	160:165	arg1	properties					179:188	strong antioxidant properties	160:188	strong antioxidant properties	160:188	In this study, hesperidin (HSP) biological agent, which has strong antioxidant properties, was successfully transferred to ZnO nanoparticles, which were first synthesized by the hydrothermal method.					
35797143	9	5	theme	6 wt	1541:1544	arg1	%					1545:1545	the composite containing 6 wt% the ZnO-HSP	1516:1557	the composite containing 6 wt% the ZnO-HSP	1516:1557	While the inhibition diameter of the CS against <i>E. coli</i> was 18.3, the same value increased to 22.3 for the composite containing 6 wt% the ZnO-HSP.					
35797143	4	6	from	measurements	783:794	arg1	29 nm					743:747	29 nm	743:747	29 nm	743:747	The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements.					
35797143	4	6	from	measurements	783:794	arg1	size					711:714	The mean particle size	693:714	The mean particle size of ZnO	693:721	The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements.					
35797143	10	7	theme	antioxidant	1582:1592	arg1	capacity					1594:1601	the antioxidant capacity	1578:1601	the antioxidant capacity of both the ZnO-HSP particles and the CS/ZnO-HSP nanocomposites	1578:1665	The HSP increased the antioxidant capacity of both the ZnO-HSP particles and the CS/ZnO-HSP nanocomposites, reducing the toxic effects of ZnO nanoparticles.					
35797143	5	8	theme	ZnO-HSPs	815:822	arg1	effect					801:806	The effect	797:806	The effect of the ZnO-HSPs on the thermal properties of pure CS	797:859	The effect of the ZnO-HSPs on the thermal properties of pure CS was investigated by thermogravimetric analysis and differential scanning calorimetry techniques, and improvements were noted in the thermal properties of CS.					
35797143	4	9	theme	mean	697:700	arg1	29 nm					743:747	29 nm	743:747	29 nm	743:747	The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements.					
35797143	4	9	theme	mean	697:700	arg1	size					711:714	The mean particle size	693:714	The mean particle size of ZnO	693:721	The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements.					
35797143	5	10	from	effect	801:806	arg1	properties					839:848	the thermal properties	827:848	the thermal properties of pure CS	827:859	The effect of the ZnO-HSPs on the thermal properties of pure CS was investigated by thermogravimetric analysis and differential scanning calorimetry techniques, and improvements were noted in the thermal properties of CS.					
35797143	3	11	theme	scanning	615:622	arg1	SEM					645:647	SEM	645:647	SEM	645:647	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	3	11	theme	scanning	615:622	arg1	microscopy					633:642	scanning electron microscopy	615:642	scanning electron microscopy (SEM)	615:648	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	1	12	theme	antioxidant	167:177	arg1	properties					179:188	strong antioxidant properties	160:188	strong antioxidant properties	160:188	In this study, hesperidin (HSP) biological agent, which has strong antioxidant properties, was successfully transferred to ZnO nanoparticles, which were first synthesized by the hydrothermal method.					
35797143	6	13	theme	ZnO-HSP	1150:1156	arg1	weight					1136:1141	weight	1136:1141	weight of the ZnO-HSP	1136:1156	While the <i>T</i><sub>g</sub> value of CS was 81 °C, this value increased by 13-94 °C with the addition of 6 wt% by weight of the ZnO-HSP.					
35797143	2	14	theme	ZnO-HSPs	390:397	arg1	ratios					376:381	different ratios	366:381	different ratios of the ZnO-HSPs	366:397	Then, chitosan (CS)/ZnO-HSP nanocomposites were produced by adding different ratios of the ZnO-HSPs to the biodegradable CS biopolymer by hydrothermal method.					
35797143	9	15	dep	%	1545:1545	arg1	ZnO-HSP					1551:1557	the ZnO-HSP	1547:1557	the composite containing 6 wt% the ZnO-HSP	1516:1557	While the inhibition diameter of the CS against <i>E. coli</i> was 18.3, the same value increased to 22.3 for the composite containing 6 wt% the ZnO-HSP.					
35797143	7	16	theme	materials	1187:1195	arg1	effect					1177:1182	The antibacterial effect	1159:1182	The antibacterial effect of materials	1159:1195	The antibacterial effect of materials was determined by the disc diffusion method.					
35797143	8	17	dep	<i>Staphylococcus	1361:1377	arg1	aureus</i>					1379:1388	aureus</i>	1379:1388	aureus</i>	1379:1388	The ZnO-HSPs added to the CS caused the nanocomposites to have a remarkable effect against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> microorganisms.					
35797143	13	18	theme	hybrid	2017:2022	arg1	system					2024:2029	this triple hybrid system	2005:2029	this triple hybrid system	2005:2029	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	12	19	theme	antibacterial	1880:1892	arg1	activity					1894:1901	antibacterial activity	1880:1901	antibacterial activity	1880:1901	The fact that the produced nanocomposites exhibit antibacterial activity and do not harm human cells shows that they can be a safe product for health.					
35797143	13	20	theme	antioxidant	2221:2231	arg1	properties					2233:2242	its antimicrobial and strong antioxidant properties	2192:2242	properties	2233:2242	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	1	21	dep	hesperidin	115:124	arg1	agent					143:147	biological agent	132:147	biological agent	132:147	In this study, hesperidin (HSP) biological agent, which has strong antioxidant properties, was successfully transferred to ZnO nanoparticles, which were first synthesized by the hydrothermal method.					
35797143	0	22	theme	chitosan	90:97	arg1	properties					76:85	the antibacterial properties	58:85	the antibacterial properties of chitosan	58:97	Synergistic effect of ZnO nanoparticles and hesperidin on the antibacterial properties of chitosan.					
35797143	2	23	theme	different	366:374	arg1	ratios					376:381	different ratios	366:381	different ratios of the ZnO-HSPs	366:397	Then, chitosan (CS)/ZnO-HSP nanocomposites were produced by adding different ratios of the ZnO-HSPs to the biodegradable CS biopolymer by hydrothermal method.					
35797143	9	24	theme	composite	1520:1528	arg1	%					1545:1545	the composite containing 6 wt% the ZnO-HSP	1516:1557	the composite containing 6 wt% the ZnO-HSP	1516:1557	While the inhibition diameter of the CS against <i>E. coli</i> was 18.3, the same value increased to 22.3 for the composite containing 6 wt% the ZnO-HSP.					
35797143	6	25	theme	CS	1059:1060	arg1	value					1050:1054	the <i>T</i><sub>g</sub> value	1025:1054	the <i>T</i><sub>g</sub> value of CS	1025:1060	While the <i>T</i><sub>g</sub> value of CS was 81 °C, this value increased by 13-94 °C with the addition of 6 wt% by weight of the ZnO-HSP.					
35797143	2	26	theme	CS	420:421	arg1	biopolymer					423:432	the biodegradable CS biopolymer	402:432	the biodegradable CS biopolymer by hydrothermal method	402:455	Then, chitosan (CS)/ZnO-HSP nanocomposites were produced by adding different ratios of the ZnO-HSPs to the biodegradable CS biopolymer by hydrothermal method.					
35797143	4	27	theme	ZnO	719:721	arg1	29 nm					743:747	29 nm	743:747	29 nm	743:747	The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements.					
35797143	4	27	theme	ZnO	719:721	arg1	size					711:714	The mean particle size	693:714	The mean particle size of ZnO	693:721	The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements.					
35797143	0	28	theme	Synergistic	0:10	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of ZnO	0:24	Synergistic effect of ZnO nanoparticles and hesperidin on the antibacterial properties of chitosan.					
35797143	5	29	theme	differential	912:923	arg1	calorimetry					934:944	differential scanning calorimetry	912:944	differential scanning calorimetry	912:944	The effect of the ZnO-HSPs on the thermal properties of pure CS was investigated by thermogravimetric analysis and differential scanning calorimetry techniques, and improvements were noted in the thermal properties of CS.					
35797143	8	30	theme	<i>Escherichia	1333:1346	arg1	microorganisms					1390:1403	<i>Escherichia coli</i> and <i>Staphylococcus aureus</i> microorganisms	1333:1403	<i>Escherichia coli</i> and <i>Staphylococcus aureus</i> microorganisms	1333:1403	The ZnO-HSPs added to the CS caused the nanocomposites to have a remarkable effect against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> microorganisms.					
35797143	10	31	theme	CS/ZnO-HSP	1641:1650	arg1	nanocomposites					1652:1665	the CS/ZnO-HSP nanocomposites	1637:1665	the CS/ZnO-HSP nanocomposites	1637:1665	The HSP increased the antioxidant capacity of both the ZnO-HSP particles and the CS/ZnO-HSP nanocomposites, reducing the toxic effects of ZnO nanoparticles.					
35797143	1	32	contain	has	156:158	arg1	hesperidin					115:124	hesperidin	115:124	hesperidin	115:124	In this study, hesperidin (HSP) biological agent, which has strong antioxidant properties, was successfully transferred to ZnO nanoparticles, which were first synthesized by the hydrothermal method.					
35797143	1	32	contain	has	156:158	arg2	properties					179:188	strong antioxidant properties	160:188	strong antioxidant properties	160:188	In this study, hesperidin (HSP) biological agent, which has strong antioxidant properties, was successfully transferred to ZnO nanoparticles, which were first synthesized by the hydrothermal method.					
35797143	1	32	contain	has	156:158	arg1	HSP					127:129	HSP	127:129	HSP	127:129	In this study, hesperidin (HSP) biological agent, which has strong antioxidant properties, was successfully transferred to ZnO nanoparticles, which were first synthesized by the hydrothermal method.					
35797143	8	33	dep	<i>Escherichia	1333:1346	arg1	coli</i>					1348:1355	coli</i>	1348:1355	coli</i>	1348:1355	The ZnO-HSPs added to the CS caused the nanocomposites to have a remarkable effect against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> microorganisms.					
35797143	1	34	theme	hydrothermal	278:289	arg1	method					291:296	the hydrothermal method	274:296	the hydrothermal method	274:296	In this study, hesperidin (HSP) biological agent, which has strong antioxidant properties, was successfully transferred to ZnO nanoparticles, which were first synthesized by the hydrothermal method.					
35797143	11	35	contain	have	1784:1787	arg1	nanocomposites					1761:1774	the CS/ZnO-HSP nanocomposites	1746:1774	the CS/ZnO-HSP nanocomposites	1746:1774	Thus, it was determined that the CS/ZnO-HSP nanocomposites did not have any cytotoxicity in healthy human cells.					
35797143	11	35	contain	have	1784:1787	arg2	cytotoxicity					1793:1804	any cytotoxicity	1789:1804	any cytotoxicity	1789:1804	Thus, it was determined that the CS/ZnO-HSP nanocomposites did not have any cytotoxicity in healthy human cells.					
35797143	11	36	theme	human	1817:1821	arg1	cells					1823:1827	healthy human cells	1809:1827	healthy human cells	1809:1827	Thus, it was determined that the CS/ZnO-HSP nanocomposites did not have any cytotoxicity in healthy human cells.					
35797143	13	37	theme	cost-effective	2142:2155	arg1	it					2045:2046	it	2045:2046	it	2045:2046	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	13	37	theme	cost-effective	2142:2155	arg1	biomaterial					2167:2177	a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial	2091:2177	a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties	2091:2242	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	5	38	theme	CS	1015:1016	arg1	properties					1001:1010	the thermal properties	989:1010	the thermal properties of CS	989:1016	The effect of the ZnO-HSPs on the thermal properties of pure CS was investigated by thermogravimetric analysis and differential scanning calorimetry techniques, and improvements were noted in the thermal properties of CS.					
35797143	3	39	theme	energy	655:660	arg1	spectroscopy					679:690	energy dispersive X-ray spectroscopy	655:690	energy dispersive X-ray spectroscopy	655:690	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	4	40	theme	XRD	758:760	arg1	calculations					762:773	the XRD calculations	754:773	the XRD calculations	754:773	The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements.					
35797143	2	41	theme	chitosan	305:312	arg1	nanocomposites					327:340	chitosan (CS)/ZnO-HSP nanocomposites	305:340	chitosan (CS)/ZnO-HSP nanocomposites	305:340	Then, chitosan (CS)/ZnO-HSP nanocomposites were produced by adding different ratios of the ZnO-HSPs to the biodegradable CS biopolymer by hydrothermal method.					
35797143	13	42	theme	naturally-sourced	2093:2109	arg1	it					2045:2046	it	2045:2046	it	2045:2046	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	13	42	theme	naturally-sourced	2093:2109	arg1	biomaterial					2167:2177	a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial	2091:2177	a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties	2091:2242	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	7	43	theme	diffusion	1224:1232	arg1	method					1234:1239	the disc diffusion method	1215:1239	the disc diffusion method	1215:1239	The antibacterial effect of materials was determined by the disc diffusion method.					
35797143	10	44	theme	ZnO	1698:1700	arg1	nanoparticles					1702:1714	ZnO nanoparticles	1698:1714	ZnO nanoparticles	1698:1714	The HSP increased the antioxidant capacity of both the ZnO-HSP particles and the CS/ZnO-HSP nanocomposites, reducing the toxic effects of ZnO nanoparticles.					
35797143	2	45	theme	/ZnO-HSP	318:325	arg1	nanocomposites					327:340	chitosan (CS)/ZnO-HSP nanocomposites	305:340	chitosan (CS)/ZnO-HSP nanocomposites	305:340	Then, chitosan (CS)/ZnO-HSP nanocomposites were produced by adding different ratios of the ZnO-HSPs to the biodegradable CS biopolymer by hydrothermal method.					
35797143	13	46	theme	biomedical	2064:2073	arg1	applications					2075:2086	biomedical applications	2064:2086	biomedical applications	2064:2086	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	3	47	theme	X-ray	673:677	arg1	spectroscopy					679:690	energy dispersive X-ray spectroscopy	655:690	energy dispersive X-ray spectroscopy	655:690	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	13	48	used	used	2056:2059	arg2	it					2045:2046	it	2045:2046	it	2045:2046	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	13	48	used	used	2056:2059	arg2	biomaterial					2167:2177	a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial	2091:2177	a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties	2091:2242	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	5	49	theme	CS	858:859	arg1	properties					839:848	the thermal properties	827:848	the thermal properties of pure CS	827:859	The effect of the ZnO-HSPs on the thermal properties of pure CS was investigated by thermogravimetric analysis and differential scanning calorimetry techniques, and improvements were noted in the thermal properties of CS.					
35797143	3	50	theme	resulting	462:470	arg1	materials					472:480	The resulting materials	458:480	The resulting materials	458:480	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	3	51	theme	biophysical	515:525	arg1	diffraction					555:565	X-ray diffraction	549:565	X-ray diffraction (XRD)	549:571	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	3	51	theme	biophysical	515:525	arg1	strategies					527:536	various biophysical strategies	507:536	various biophysical strategies	507:536	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	3	52	dep	Fourier	574:580	arg1	transform					582:590	transform	582:590	transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy	582:690	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	9	53	theme	inhibition	1416:1425	arg1	diameter					1427:1434	the inhibition diameter	1412:1434	the inhibition diameter of the CS against <i>E. coli</i>	1412:1467	While the inhibition diameter of the CS against <i>E. coli</i> was 18.3, the same value increased to 22.3 for the composite containing 6 wt% the ZnO-HSP.					
35797143	9	53	theme	inhibition	1416:1425	arg1	18.3					1473:1476	18.3	1473:1476	18.3	1473:1476	While the inhibition diameter of the CS against <i>E. coli</i> was 18.3, the same value increased to 22.3 for the composite containing 6 wt% the ZnO-HSP.					
35797143	12	54	theme	safe	1956:1959	arg1	product					1961:1967	a safe product	1954:1967	a safe product for health	1954:1978	The fact that the produced nanocomposites exhibit antibacterial activity and do not harm human cells shows that they can be a safe product for health.					
35797143	12	54	theme	safe	1956:1959	arg1	they					1942:1945	they	1942:1945	they	1942:1945	The fact that the produced nanocomposites exhibit antibacterial activity and do not harm human cells shows that they can be a safe product for health.					
35797143	6	55	theme	%	1131:1131	arg1	addition					1115:1122	the addition	1111:1122	the addition of 6 wt%	1111:1131	While the <i>T</i><sub>g</sub> value of CS was 81 °C, this value increased by 13-94 °C with the addition of 6 wt% by weight of the ZnO-HSP.					
35797143	5	56	theme	thermal	831:837	arg1	properties					839:848	the thermal properties	827:848	the thermal properties of pure CS	827:859	The effect of the ZnO-HSPs on the thermal properties of pure CS was investigated by thermogravimetric analysis and differential scanning calorimetry techniques, and improvements were noted in the thermal properties of CS.					
35797143	3	57	theme	X-ray	549:553	arg1	Fourier					574:580	Fourier	574:580	Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy	574:690	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	3	57	theme	X-ray	549:553	arg1	diffraction					555:565	X-ray diffraction	549:565	X-ray diffraction (XRD)	549:571	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	3	57	theme	X-ray	549:553	arg1	XRD					568:570	XRD	568:570	XRD	568:570	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	9	58	theme	CS	1443:1444	arg1	diameter					1427:1434	the inhibition diameter	1412:1434	the inhibition diameter of the CS against <i>E. coli</i>	1412:1467	While the inhibition diameter of the CS against <i>E. coli</i> was 18.3, the same value increased to 22.3 for the composite containing 6 wt% the ZnO-HSP.					
35797143	9	58	theme	CS	1443:1444	arg1	18.3					1473:1476	18.3	1473:1476	18.3	1473:1476	While the inhibition diameter of the CS against <i>E. coli</i> was 18.3, the same value increased to 22.3 for the composite containing 6 wt% the ZnO-HSP.					
35797143	11	59	theme	CS/ZnO-HSP	1750:1759	arg1	nanocomposites					1761:1774	the CS/ZnO-HSP nanocomposites	1746:1774	the CS/ZnO-HSP nanocomposites	1746:1774	Thus, it was determined that the CS/ZnO-HSP nanocomposites did not have any cytotoxicity in healthy human cells.					
35797143	3	60	dep	transform	582:590	arg1	infrared					592:599	infrared	592:599	transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy	582:690	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	9	61	theme	<i>E.	1454:1458	arg1	coli</i>					1460:1467	<i>E. coli</i>	1454:1467	<i>E. coli</i>	1454:1467	While the inhibition diameter of the CS against <i>E. coli</i> was 18.3, the same value increased to 22.3 for the composite containing 6 wt% the ZnO-HSP.					
35797143	12	62	theme	human	1919:1923	arg1	cells					1925:1929	human cells	1919:1929	human cells	1919:1929	The fact that the produced nanocomposites exhibit antibacterial activity and do not harm human cells shows that they can be a safe product for health.					
35797143	5	63	theme	thermogravimetric	881:897	arg1	analysis					899:906	thermogravimetric analysis	881:906	thermogravimetric analysis	881:906	The effect of the ZnO-HSPs on the thermal properties of pure CS was investigated by thermogravimetric analysis and differential scanning calorimetry techniques, and improvements were noted in the thermal properties of CS.					
35797143	4	64	theme	particle	702:709	arg1	29 nm					743:747	29 nm	743:747	29 nm	743:747	The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements.					
35797143	4	64	theme	particle	702:709	arg1	size					711:714	The mean particle size	693:714	The mean particle size of ZnO	693:721	The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements.					
35797143	0	65	theme	antibacterial	62:74	arg1	properties					76:85	the antibacterial properties	58:85	the antibacterial properties of chitosan	58:97	Synergistic effect of ZnO nanoparticles and hesperidin on the antibacterial properties of chitosan.					
35797143	4	66	from	calculations	762:773	arg1	29 nm					743:747	29 nm	743:747	29 nm	743:747	The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements.					
35797143	4	66	from	calculations	762:773	arg1	size					711:714	The mean particle size	693:714	The mean particle size of ZnO	693:721	The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements.					
35797143	9	67	theme	same	1483:1486	arg1	value					1488:1492	the same value	1479:1492	the same value	1479:1492	While the inhibition diameter of the CS against <i>E. coli</i> was 18.3, the same value increased to 22.3 for the composite containing 6 wt% the ZnO-HSP.					
35797143	7	68	theme	antibacterial	1163:1175	arg1	effect					1177:1182	The antibacterial effect	1159:1182	The antibacterial effect of materials	1159:1195	The antibacterial effect of materials was determined by the disc diffusion method.					
35797143	13	69	theme	triple	2010:2015	arg1	system					2024:2029	this triple hybrid system	2005:2029	this triple hybrid system	2005:2029	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	1	70	theme	biological	132:141	arg1	agent					143:147	biological agent	132:147	biological agent	132:147	In this study, hesperidin (HSP) biological agent, which has strong antioxidant properties, was successfully transferred to ZnO nanoparticles, which were first synthesized by the hydrothermal method.					
35797143	10	71	theme	ZnO-HSP	1615:1621	arg1	particles					1623:1631	the ZnO-HSP particles	1611:1631	the ZnO-HSP particles	1611:1631	The HSP increased the antioxidant capacity of both the ZnO-HSP particles and the CS/ZnO-HSP nanocomposites, reducing the toxic effects of ZnO nanoparticles.					
35797143	5	72	dep	analysis	899:906	arg1	techniques					946:955	techniques	946:955	techniques	946:955	The effect of the ZnO-HSPs on the thermal properties of pure CS was investigated by thermogravimetric analysis and differential scanning calorimetry techniques, and improvements were noted in the thermal properties of CS.					
35797143	13	73	theme	strong	2214:2219	arg1	properties					2233:2242	its antimicrobial and strong antioxidant properties	2192:2242	properties	2233:2242	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	10	74	theme	nanocomposites	1652:1665	arg1	capacity					1594:1601	the antioxidant capacity	1578:1601	the antioxidant capacity of both the ZnO-HSP particles and the CS/ZnO-HSP nanocomposites	1578:1665	The HSP increased the antioxidant capacity of both the ZnO-HSP particles and the CS/ZnO-HSP nanocomposites, reducing the toxic effects of ZnO nanoparticles.					
35797143	12	75	theme	produced	1848:1855	arg1	nanocomposites					1857:1870	the produced nanocomposites	1844:1870	the produced nanocomposites	1844:1870	The fact that the produced nanocomposites exhibit antibacterial activity and do not harm human cells shows that they can be a safe product for health.					
35797143	9	76	theme	containing	1530:1539	arg1	%					1545:1545	the composite containing 6 wt% the ZnO-HSP	1516:1557	the composite containing 6 wt% the ZnO-HSP	1516:1557	While the inhibition diameter of the CS against <i>E. coli</i> was 18.3, the same value increased to 22.3 for the composite containing 6 wt% the ZnO-HSP.					
35797143	2	77	theme	biodegradable	406:418	arg1	biopolymer					423:432	the biodegradable CS biopolymer	402:432	the biodegradable CS biopolymer by hydrothermal method	402:455	Then, chitosan (CS)/ZnO-HSP nanocomposites were produced by adding different ratios of the ZnO-HSPs to the biodegradable CS biopolymer by hydrothermal method.					
35797143	8	78	theme	remarkable	1307:1316	arg1	effect					1318:1323	a remarkable effect	1305:1323	a remarkable effect	1305:1323	The ZnO-HSPs added to the CS caused the nanocomposites to have a remarkable effect against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> microorganisms.					
35797143	0	79	theme	ZnO	22:24	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of ZnO	0:24	Synergistic effect of ZnO nanoparticles and hesperidin on the antibacterial properties of chitosan.					
35797143	5	80	theme	scanning	925:932	arg1	calorimetry					934:944	differential scanning calorimetry	912:944	differential scanning calorimetry	912:944	The effect of the ZnO-HSPs on the thermal properties of pure CS was investigated by thermogravimetric analysis and differential scanning calorimetry techniques, and improvements were noted in the thermal properties of CS.					
35797143	6	81	theme	<i>T</i><sub>g</sub>	1029:1048	arg1	value					1050:1054	the <i>T</i><sub>g</sub> value	1025:1054	the <i>T</i><sub>g</sub> value of CS	1025:1060	While the <i>T</i><sub>g</sub> value of CS was 81 °C, this value increased by 13-94 °C with the addition of 6 wt% by weight of the ZnO-HSP.					
35797143	5	82	theme	thermal	993:999	arg1	properties					1001:1010	the thermal properties	989:1010	the thermal properties of CS	989:1016	The effect of the ZnO-HSPs on the thermal properties of pure CS was investigated by thermogravimetric analysis and differential scanning calorimetry techniques, and improvements were noted in the thermal properties of CS.					
35797143	2	83	theme	hydrothermal	437:448	arg1	method					450:455	hydrothermal method	437:455	hydrothermal method	437:455	Then, chitosan (CS)/ZnO-HSP nanocomposites were produced by adding different ratios of the ZnO-HSPs to the biodegradable CS biopolymer by hydrothermal method.					
35797143	3	84	theme	electron	624:631	arg1	SEM					645:647	SEM	645:647	SEM	645:647	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	3	84	theme	electron	624:631	arg1	microscopy					633:642	scanning electron microscopy	615:642	scanning electron microscopy (SEM)	615:648	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	4	85	theme	SEM	779:781	arg1	measurements					783:794	SEM measurements	779:794	SEM measurements	779:794	The mean particle size of ZnO was estimated to be 29 nm from the XRD calculations and SEM measurements.					
35797143	10	86	theme	particles	1623:1631	arg1	capacity					1594:1601	the antioxidant capacity	1578:1601	the antioxidant capacity of both the ZnO-HSP particles and the CS/ZnO-HSP nanocomposites	1578:1665	The HSP increased the antioxidant capacity of both the ZnO-HSP particles and the CS/ZnO-HSP nanocomposites, reducing the toxic effects of ZnO nanoparticles.					
35797143	8	87	theme	<i>Staphylococcus	1361:1377	arg1	microorganisms					1390:1403	<i>Escherichia coli</i> and <i>Staphylococcus aureus</i> microorganisms	1333:1403	<i>Escherichia coli</i> and <i>Staphylococcus aureus</i> microorganisms	1333:1403	The ZnO-HSPs added to the CS caused the nanocomposites to have a remarkable effect against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> microorganisms.					
35797143	13	88	theme	composite	2157:2165	arg1	it					2045:2046	it	2045:2046	it	2045:2046	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	13	88	theme	composite	2157:2165	arg1	biomaterial					2167:2177	a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial	2091:2177	a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties	2091:2242	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	7	89	theme	disc	1219:1222	arg1	method					1234:1239	the disc diffusion method	1215:1239	the disc diffusion method	1215:1239	The antibacterial effect of materials was determined by the disc diffusion method.					
35797143	11	90	theme	healthy	1809:1815	arg1	cells					1823:1827	healthy human cells	1809:1827	healthy human cells	1809:1827	Thus, it was determined that the CS/ZnO-HSP nanocomposites did not have any cytotoxicity in healthy human cells.					
35797143	5	91	theme	pure	853:856	arg1	CS					858:859	pure CS	853:859	pure CS	853:859	The effect of the ZnO-HSPs on the thermal properties of pure CS was investigated by thermogravimetric analysis and differential scanning calorimetry techniques, and improvements were noted in the thermal properties of CS.					
35797143	13	92	theme	friendly	2128:2135	arg1	it					2045:2046	it	2045:2046	it	2045:2046	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	13	92	theme	friendly	2128:2135	arg1	biomaterial					2167:2177	a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial	2091:2177	a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties	2091:2242	From all these results, this triple hybrid system is hoped that it will be used in biomedical applications as a naturally-sourced, environmentally friendly, and cost-effective composite biomaterial by combining its antimicrobial and strong antioxidant properties.					
35797143	3	93	theme	various	507:513	arg1	diffraction					555:565	X-ray diffraction	549:565	X-ray diffraction (XRD)	549:571	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	3	93	theme	various	507:513	arg1	strategies					527:536	various biophysical strategies	507:536	various biophysical strategies	507:536	The resulting materials were characterized using various biophysical strategies, including X-ray diffraction (XRD), Fourier transform infrared spectrometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy.					
35797143	1	94	theme	ZnO	223:225	arg1	nanoparticles					227:239	ZnO nanoparticles	223:239	ZnO nanoparticles	223:239	In this study, hesperidin (HSP) biological agent, which has strong antioxidant properties, was successfully transferred to ZnO nanoparticles, which were first synthesized by the hydrothermal method.					
35545061	8	0	theme	CS-rGO	1463:1468	arg1	coating					1470:1476	the CS-rGO coating	1459:1476	the CS-rGO coating	1459:1476	Overall, the CS-rGO coating adjusts CPP scaffolds' biological environment interface and endows CPP scaffolds with more bioactivity.					
35545061	0	1	from	construction	4:15	arg1	scaffolds					122:130	porous calcium polyphosphate scaffolds	93:130	porous calcium polyphosphate scaffolds for bone tissue engineering	93:158	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.					
35545061	1	2	from	regeneration	166:177	arg1	defects					193:199	large bone defects	182:199	large bone defects	182:199	Bone regeneration in large bone defects remains one of the major challenges in orthopedic surgery.					
35545061	7	3	theme	lesser	1387:1392	arg1	activator					1403:1411	lesser receptor activator	1387:1411	lesser receptor activator of nuclear factor-κB ligand (RANKL)	1387:1447	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	0	4	theme	calcium	100:106	arg1	scaffolds					122:130	porous calcium polyphosphate scaffolds	93:130	porous calcium polyphosphate scaffolds for bone tissue engineering	93:158	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.					
35545061	4	5	theme	CPP	535:537	arg1	scaffolds					539:547	CPP scaffolds	535:547	CPP scaffolds	535:547	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	5	6	theme	antibacterial	971:983	arg1	activity					985:992	considerable antibacterial activity	958:992	considerable antibacterial activity	958:992	Our results showed that the obtained CPP/CS-rGO composite scaffolds exhibited highly improved biomineralization and considerable antibacterial activity.					
35545061	8	7	theme	environment	1512:1522	arg1	interface					1524:1532	CPP scaffolds' biological environment interface	1486:1532	CPP scaffolds' biological environment interface	1486:1532	Overall, the CS-rGO coating adjusts CPP scaffolds' biological environment interface and endows CPP scaffolds with more bioactivity.					
35545061	3	8	theme	osteoinductive	413:426	arg1	activity					428:435	osteoinductive activity	413:435	osteoinductive activity	413:435	However, the present CPP scaffolds lack enough osteoinductive activity to facilitate bone regeneration at bone defects that exceed the critical size threshold.					
35545061	0	9	theme	porous	93:98	arg1	scaffolds					122:130	porous calcium polyphosphate scaffolds	93:130	porous calcium polyphosphate scaffolds for bone tissue engineering	93:158	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.					
35545061	7	10	theme	interfacial	1253:1263	arg1	osseointegration					1265:1280	interfacial osseointegration	1253:1280	interfacial osseointegration	1253:1280	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	4	11	theme	electrostatic	796:808	arg1	interaction					810:820	strong electrostatic interaction	789:820	strong electrostatic interaction	789:820	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	3	12	theme	size	510:513	arg1	threshold					515:523	the critical size threshold	497:523	the critical size threshold	497:523	However, the present CPP scaffolds lack enough osteoinductive activity to facilitate bone regeneration at bone defects that exceed the critical size threshold.					
35545061	5	13	theme	composite	890:898	arg1	scaffolds					900:908	the obtained CPP/CS-rGO composite scaffolds	866:908	the obtained CPP/CS-rGO composite scaffolds	866:908	Our results showed that the obtained CPP/CS-rGO composite scaffolds exhibited highly improved biomineralization and considerable antibacterial activity.					
35545061	4	14	theme	hydrogen	826:833	arg1	bonds					835:839	hydrogen bonds	826:839	hydrogen bonds	826:839	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	1	15	theme	large	182:186	arg1	defects					193:199	large bone defects	182:199	large bone defects	182:199	Bone regeneration in large bone defects remains one of the major challenges in orthopedic surgery.					
35545061	0	16	theme	polyphosphate	108:120	arg1	scaffolds					122:130	porous calcium polyphosphate scaffolds	93:130	porous calcium polyphosphate scaffolds for bone tissue engineering	93:158	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.					
35545061	7	17	theme	marrow	1307:1312	arg1	cells					1331:1335	bone marrow mesenchymal stem cells	1302:1335	bone marrow mesenchymal stem cells (BMSCs)	1302:1343	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	7	17	theme	marrow	1307:1312	arg1	BMSCs					1338:1342	BMSCs	1338:1342	BMSCs	1338:1342	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	6	18	theme	osteogenesis-related	1140:1159	arg1	proteinsin					1161:1170	osteogenesis-related proteinsin	1140:1170	osteogenesis-related proteinsin vitro	1140:1176	More importantly, CPP/CS-rGO composite scaffolds could drive osteogenic differentiation of BMSCs and significantly up-regulate the expression of osteogenesis-related proteinsin vitro.					
35545061	7	19	theme	aseptic	1223:1229	arg1	loosening					1231:1239	aseptic loosening	1223:1239	aseptic loosening	1223:1239	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	1	20	theme	bone	188:191	arg1	defects					193:199	large bone defects	182:199	large bone defects	182:199	Bone regeneration in large bone defects remains one of the major challenges in orthopedic surgery.					
35545061	6	21	theme	osteogenic	1056:1065	arg1	differentiation					1067:1081	osteogenic differentiation	1056:1081	osteogenic differentiation of BMSCs	1056:1090	More importantly, CPP/CS-rGO composite scaffolds could drive osteogenic differentiation of BMSCs and significantly up-regulate the expression of osteogenesis-related proteinsin vitro.					
35545061	4	22	theme	oxide	696:700	arg1	sheets					711:716	chitosan-grafted reduced graphene oxide (CS-rGO) sheets	662:716	chitosan-grafted reduced graphene oxide (CS-rGO) sheets	662:716	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	4	23	theme	scaffolds	771:779	arg1	surface					756:762	the surface	752:762	the surface of CPP scaffolds	752:779	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	7	24	theme	nuclear	1416:1422	arg1	RANKL					1442:1446	RANKL	1442:1446	RANKL	1442:1446	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	7	24	theme	nuclear	1416:1422	arg1	ligand					1434:1439	nuclear factor-κB ligand	1416:1439	nuclear factor-κB ligand (RANKL)	1416:1447	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	3	25	theme	bone	451:454	arg1	regeneration					456:467	bone regeneration	451:467	bone regeneration	451:467	However, the present CPP scaffolds lack enough osteoinductive activity to facilitate bone regeneration at bone defects that exceed the critical size threshold.					
35545061	6	26	theme	proteinsin	1161:1170	arg1	expression					1126:1135	the expression	1122:1135	the expression of osteogenesis-related proteinsin vitro	1122:1176	More importantly, CPP/CS-rGO composite scaffolds could drive osteogenic differentiation of BMSCs and significantly up-regulate the expression of osteogenesis-related proteinsin vitro.					
35545061	4	27	theme	better	591:596	arg1	regeneration					603:614	better bone regeneration	591:614	better bone regeneration	591:614	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	6	28	theme	composite	1024:1032	arg1	scaffolds					1034:1042	CPP/CS-rGO composite scaffolds	1013:1042	CPP/CS-rGO composite scaffolds	1013:1042	More importantly, CPP/CS-rGO composite scaffolds could drive osteogenic differentiation of BMSCs and significantly up-regulate the expression of osteogenesis-related proteinsin vitro.					
35545061	7	29	theme	CS-rGO	1194:1199	arg1	Meanwhile					1179:1187	Meanwhile	1179:1187	Meanwhile	1179:1187	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	7	29	theme	CS-rGO	1194:1199	arg1	coating					1201:1207	the CS-rGO coating	1190:1207	the CS-rGO coating	1190:1207	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	4	30	theme	CPP	767:769	arg1	scaffolds					771:779	CPP scaffolds	767:779	CPP scaffolds	767:779	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	3	31	theme	CPP	387:389	arg1	scaffolds					391:399	the present CPP scaffolds	375:399	the present CPP scaffolds	375:399	However, the present CPP scaffolds lack enough osteoinductive activity to facilitate bone regeneration at bone defects that exceed the critical size threshold.					
35545061	0	32	theme	bone	136:139	arg1	engineering					148:158	bone tissue engineering	136:158	bone tissue engineering	136:158	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.					
35545061	4	33	theme	strong	789:794	arg1	interaction					810:820	strong electrostatic interaction	789:820	strong electrostatic interaction	789:820	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	4	34	theme	chitosan-grafted	662:677	arg1	sheets					711:716	chitosan-grafted reduced graphene oxide (CS-rGO) sheets	662:716	chitosan-grafted reduced graphene oxide (CS-rGO) sheets	662:716	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	5	35	theme	obtained	870:877	arg1	scaffolds					900:908	the obtained CPP/CS-rGO composite scaffolds	866:908	the obtained CPP/CS-rGO composite scaffolds	866:908	Our results showed that the obtained CPP/CS-rGO composite scaffolds exhibited highly improved biomineralization and considerable antibacterial activity.					
35545061	2	36	theme	excellent	306:314	arg1	biocompatibility					316:331	excellent biocompatibility	306:331	excellent biocompatibility	306:331	Calcium polyphosphate (CPP) scaffolds possess excellent biocompatibility and exhibits good bone ingrowth.					
35545061	7	37	theme	mesenchymal	1314:1324	arg1	cells					1331:1335	bone marrow mesenchymal stem cells	1302:1335	bone marrow mesenchymal stem cells (BMSCs)	1302:1343	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	7	37	theme	mesenchymal	1314:1324	arg1	BMSCs					1338:1342	BMSCs	1338:1342	BMSCs	1338:1342	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	5	38	theme	improved	927:934	arg1	biomineralization					936:952	highly improved biomineralization	920:952	highly improved biomineralization	920:952	Our results showed that the obtained CPP/CS-rGO composite scaffolds exhibited highly improved biomineralization and considerable antibacterial activity.					
35545061	0	39	theme	coating	37:43	arg1	construction					4:15	The construction	0:15	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.	0:159	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.					
35545061	4	40	theme	bone	598:601	arg1	regeneration					603:614	better bone regeneration	591:614	better bone regeneration	591:614	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	7	41	theme	receptor	1394:1401	arg1	activator					1403:1411	lesser receptor activator	1387:1411	lesser receptor activator of nuclear factor-κB ligand (RANKL)	1387:1447	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	4	42	with	coating	649:655	arg1	sheets					711:716	chitosan-grafted reduced graphene oxide (CS-rGO) sheets	662:716	chitosan-grafted reduced graphene oxide (CS-rGO) sheets	662:716	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	4	43	theme	graphene	687:694	arg1	CS-rGO					703:708	CS-rGO	703:708	CS-rGO	703:708	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	4	43	theme	graphene	687:694	arg1	oxide					696:700	reduced graphene oxide	679:700	chitosan-grafted reduced graphene oxide (CS-rGO) sheets	662:716	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	0	44	theme	self-assembled	22:35	arg1	coating					37:43	a self-assembled coating	20:43	a self-assembled coating with chitosan-grafted reduced graphene oxide	20:88	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.					
35545061	4	45	theme	improved	554:561	arg1	activity					578:585	improved osteoinductive activity	554:585	improved osteoinductive activity for better bone regeneration	554:614	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	0	46	theme	tissue	141:146	arg1	engineering					148:158	bone tissue engineering	136:158	bone tissue engineering	136:158	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.					
35545061	8	47	theme	biological	1501:1510	arg1	environment					1512:1522	CPP scaffolds' biological environment	1486:1522	CPP scaffolds' biological environment interface	1486:1532	Overall, the CS-rGO coating adjusts CPP scaffolds' biological environment interface and endows CPP scaffolds with more bioactivity.					
35545061	7	48	theme	stem	1326:1329	arg1	cells					1331:1335	bone marrow mesenchymal stem cells	1302:1335	bone marrow mesenchymal stem cells (BMSCs)	1302:1343	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	7	48	theme	stem	1326:1329	arg1	BMSCs					1338:1342	BMSCs	1338:1342	BMSCs	1338:1342	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	1	49	theme	major	220:224	arg1	challenges					226:235	the major challenges	216:235	the major challenges in orthopedic surgery	216:257	Bone regeneration in large bone defects remains one of the major challenges in orthopedic surgery.					
35545061	0	50	theme	chitosan-grafted	50:65	arg1	oxide					84:88	chitosan-grafted reduced graphene oxide	50:88	chitosan-grafted reduced graphene oxide	50:88	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.					
35545061	6	51	theme	BMSCs	1086:1090	arg1	differentiation					1067:1081	osteogenic differentiation	1056:1081	osteogenic differentiation of BMSCs	1056:1090	More importantly, CPP/CS-rGO composite scaffolds could drive osteogenic differentiation of BMSCs and significantly up-regulate the expression of osteogenesis-related proteinsin vitro.					
35545061	7	52	theme	factor-κB	1424:1432	arg1	RANKL					1442:1446	RANKL	1442:1446	RANKL	1442:1446	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	7	52	theme	factor-κB	1424:1432	arg1	ligand					1434:1439	nuclear factor-κB ligand	1416:1439	nuclear factor-κB ligand (RANKL)	1416:1447	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	7	53	theme	bone	1302:1305	arg1	marrow					1307:1312	bone marrow	1302:1312	bone marrow mesenchymal stem cells (BMSCs)	1302:1343	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	8	54	theme	CPP	1545:1547	arg1	scaffolds					1549:1557	CPP scaffolds	1545:1557	CPP scaffolds	1545:1557	Overall, the CS-rGO coating adjusts CPP scaffolds' biological environment interface and endows CPP scaffolds with more bioactivity.					
35545061	7	55	theme	more	1356:1359	arg1	OPG					1378:1380	OPG	1378:1380	OPG	1378:1380	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	7	55	theme	more	1356:1359	arg1	osteoprotegerin					1361:1375	more osteoprotegerin	1356:1375	more osteoprotegerin (OPG)	1356:1381	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	7	56	theme	ligand	1434:1439	arg1	activator					1403:1411	lesser receptor activator	1387:1411	lesser receptor activator of nuclear factor-κB ligand (RANKL)	1387:1447	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	7	56	theme	ligand	1434:1439	arg1	OPG					1378:1380	OPG	1378:1380	OPG	1378:1380	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	7	56	theme	ligand	1434:1439	arg1	osteoprotegerin					1361:1375	more osteoprotegerin	1356:1375	more osteoprotegerin (OPG)	1356:1381	Meanwhile, the CS-rGO coating could inhibit aseptic loosening and improve interfacial osseointegration through stimulating bone marrow mesenchymal stem cells (BMSCs) to secrete more osteoprotegerin (OPG) and lesser receptor activator of nuclear factor-κB ligand (RANKL).					
35545061	4	57	theme	osteoinductive	563:576	arg1	activity					578:585	improved osteoinductive activity	554:585	improved osteoinductive activity for better bone regeneration	554:614	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	1	58	from	challenges	226:235	arg1	surgery					251:257	orthopedic surgery	240:257	orthopedic surgery	240:257	Bone regeneration in large bone defects remains one of the major challenges in orthopedic surgery.					
35545061	8	59	theme	more	1564:1567	arg1	bioactivity					1569:1579	more bioactivity	1564:1579	more bioactivity	1564:1579	Overall, the CS-rGO coating adjusts CPP scaffolds' biological environment interface and endows CPP scaffolds with more bioactivity.					
35545061	2	60	theme	bone	351:354	arg1	ingrowth					356:363	good bone ingrowth	346:363	good bone ingrowth	346:363	Calcium polyphosphate (CPP) scaffolds possess excellent biocompatibility and exhibits good bone ingrowth.					
35545061	1	61	theme	challenges	226:235	arg1	challenges					226:235	the major challenges	216:235	the major challenges in orthopedic surgery	216:257	Bone regeneration in large bone defects remains one of the major challenges in orthopedic surgery.					
35545061	1	61	theme	challenges	226:235	arg1	one					209:211	one	209:211	one	209:211	Bone regeneration in large bone defects remains one of the major challenges in orthopedic surgery.					
35545061	8	62	theme	CPP	1486:1488	arg1	environment					1512:1522	CPP scaffolds' biological environment	1486:1522	CPP scaffolds' biological environment interface	1486:1532	Overall, the CS-rGO coating adjusts CPP scaffolds' biological environment interface and endows CPP scaffolds with more bioactivity.					
35545061	2	63	theme	good	346:349	arg1	ingrowth					356:363	good bone ingrowth	346:363	good bone ingrowth	346:363	Calcium polyphosphate (CPP) scaffolds possess excellent biocompatibility and exhibits good bone ingrowth.					
35545061	3	64	theme	critical	501:508	arg1	threshold					515:523	the critical size threshold	497:523	the critical size threshold	497:523	However, the present CPP scaffolds lack enough osteoinductive activity to facilitate bone regeneration at bone defects that exceed the critical size threshold.					
35545061	0	65	theme	graphene	75:82	arg1	oxide					84:88	chitosan-grafted reduced graphene oxide	50:88	chitosan-grafted reduced graphene oxide	50:88	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.					
35545061	4	66	theme	reduced	679:685	arg1	CS-rGO					703:708	CS-rGO	703:708	CS-rGO	703:708	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	4	66	theme	reduced	679:685	arg1	oxide					696:700	reduced graphene oxide	679:700	chitosan-grafted reduced graphene oxide (CS-rGO) sheets	662:716	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	2	67	theme	Calcium	260:266	arg1	CPP					283:285	CPP	283:285	CPP	283:285	Calcium polyphosphate (CPP) scaffolds possess excellent biocompatibility and exhibits good bone ingrowth.					
35545061	2	67	theme	Calcium	260:266	arg1	polyphosphate					268:280	Calcium polyphosphate	260:280	Calcium polyphosphate (CPP)	260:286	Calcium polyphosphate (CPP) scaffolds possess excellent biocompatibility and exhibits good bone ingrowth.					
35545061	5	68	theme	CPP/CS-rGO	879:888	arg1	scaffolds					900:908	the obtained CPP/CS-rGO composite scaffolds	866:908	the obtained CPP/CS-rGO composite scaffolds	866:908	Our results showed that the obtained CPP/CS-rGO composite scaffolds exhibited highly improved biomineralization and considerable antibacterial activity.					
35545061	5	69	theme	considerable	958:969	arg1	activity					985:992	considerable antibacterial activity	958:992	considerable antibacterial activity	958:992	Our results showed that the obtained CPP/CS-rGO composite scaffolds exhibited highly improved biomineralization and considerable antibacterial activity.					
35545061	3	70	theme	bone	472:475	arg1	defects					477:483	bone defects	472:483	bone defects that exceed the critical size threshold	472:523	However, the present CPP scaffolds lack enough osteoinductive activity to facilitate bone regeneration at bone defects that exceed the critical size threshold.					
35545061	1	71	theme	orthopedic	240:249	arg1	surgery					251:257	orthopedic surgery	240:257	orthopedic surgery	240:257	Bone regeneration in large bone defects remains one of the major challenges in orthopedic surgery.					
35545061	0	72	theme	reduced	67:73	arg1	oxide					84:88	chitosan-grafted reduced graphene oxide	50:88	chitosan-grafted reduced graphene oxide	50:88	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.					
35545061	6	73	theme	CPP/CS-rGO	1013:1022	arg1	scaffolds					1034:1042	CPP/CS-rGO composite scaffolds	1013:1042	CPP/CS-rGO composite scaffolds	1013:1042	More importantly, CPP/CS-rGO composite scaffolds could drive osteogenic differentiation of BMSCs and significantly up-regulate the expression of osteogenesis-related proteinsin vitro.					
35545061	4	74	theme	self-assembled	634:647	arg1	coating					649:655	a self-assembled coating	632:655	a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets	632:716	To endow CPP scaffolds with improved osteoinductive activity for better bone regeneration, in this study, a self-assembled coating with chitosan-grafted reduced graphene oxide (CS-rGO) sheets was successfully constructed onto the surface of CPP scaffolds through strong electrostatic interaction and hydrogen bonds.					
35545061	0	75	with	coating	37:43	arg1	oxide					84:88	chitosan-grafted reduced graphene oxide	50:88	chitosan-grafted reduced graphene oxide	50:88	The construction of a self-assembled coating with chitosan-grafted reduced graphene oxide on porous calcium polyphosphate scaffolds for bone tissue engineering.					
35545061	1	76	theme	Bone	161:164	arg1	regeneration					166:177	Bone regeneration	161:177	Bone regeneration in large bone defects	161:199	Bone regeneration in large bone defects remains one of the major challenges in orthopedic surgery.					
35545061	3	77	theme	present	379:385	arg1	scaffolds					391:399	the present CPP scaffolds	375:399	the present CPP scaffolds	375:399	However, the present CPP scaffolds lack enough osteoinductive activity to facilitate bone regeneration at bone defects that exceed the critical size threshold.					
36500418	7	0	from	growth	1397:1402	arg1	microenvironment					1450:1465	a three-dimensional (3D) microenvironment	1425:1465	a three-dimensional (3D) microenvironment	1425:1465	Compared to the CMCS/Gel hydrogel, the incorporation of PEDOT nanoparticles significantly improved the adhesion of NSCs, and supported long-term cell growth and proliferation in a three-dimensional (3D) microenvironment.					
36500418	9	1	theme	neural	1764:1769	arg1	repair					1778:1783	neural tissue repair	1764:1783	neural tissue repair	1764:1783	These results suggest that CMCS/Gel-PEDOT hydrogels may be an attractive conductive substrate for further studies on neural tissue repair and regeneration.					
36500418	3	2	theme	hydrogel	783:790	arg1	samples					792:798	the prepared hydrogel samples	770:798	the prepared hydrogel samples	770:798	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	1	3	theme	electroactive	311:323	arg1	materials					325:333	the electroactive materials	307:333	the electroactive materials	307:333	Tissue engineering scaffolds provide biological and physiochemical cures to guide tissue recovery, and electrical signals through the electroactive materials possess tremendous potential to modulate the cell fate.					
36500418	6	4	theme	×	1234:1234	arg1	±					1226:1226	1.52 ± 0.15	1221:1231	1.52 ± 0.15	1221:1231	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	6	4	theme	×	1234:1234	arg1	S/cm					1241:1244	(1.52 ± 0.15) × 10-3 S/cm	1220:1244	(1.52 ± 0.15) × 10-3 S/cm	1220:1244	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	1	5	theme	biological	214:223	arg1	cures					244:248	biological and physiochemical cures	214:248	biological and physiochemical cures	214:248	Tissue engineering scaffolds provide biological and physiochemical cures to guide tissue recovery, and electrical signals through the electroactive materials possess tremendous potential to modulate the cell fate.					
36500418	1	6	theme	physiochemical	229:242	arg1	cures					244:248	biological and physiochemical cures	214:248	biological and physiochemical cures	214:248	Tissue engineering scaffolds provide biological and physiochemical cures to guide tissue recovery, and electrical signals through the electroactive materials possess tremendous potential to modulate the cell fate.					
36500418	0	7	theme	Neuronal	151:158	arg1	Differentiation					160:174	Neuronal Differentiation	151:174	Neuronal Differentiation	151:174	Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation.					
36500418	1	8	theme	tissue	259:264	arg1	recovery					266:273	tissue recovery	259:273	tissue recovery	259:273	Tissue engineering scaffolds provide biological and physiochemical cures to guide tissue recovery, and electrical signals through the electroactive materials possess tremendous potential to modulate the cell fate.					
36500418	7	9	theme	three-dimensional	1427:1443	arg1	microenvironment					1450:1465	a three-dimensional (3D) microenvironment	1425:1465	a three-dimensional (3D) microenvironment	1425:1465	Compared to the CMCS/Gel hydrogel, the incorporation of PEDOT nanoparticles significantly improved the adhesion of NSCs, and supported long-term cell growth and proliferation in a three-dimensional (3D) microenvironment.					
36500418	9	10	theme	CMCS/Gel-PEDOT	1674:1687	arg1	substrate					1731:1739	an attractive conductive substrate	1706:1739	an attractive conductive substrate for further studies on neural tissue repair and regeneration	1706:1800	These results suggest that CMCS/Gel-PEDOT hydrogels may be an attractive conductive substrate for further studies on neural tissue repair and regeneration.					
36500418	9	10	theme	CMCS/Gel-PEDOT	1674:1687	arg1	hydrogels					1689:1697	CMCS/Gel-PEDOT hydrogels	1674:1697	CMCS/Gel-PEDOT hydrogels	1674:1697	These results suggest that CMCS/Gel-PEDOT hydrogels may be an attractive conductive substrate for further studies on neural tissue repair and regeneration.					
36500418	5	11	theme	water	1004:1008	arg1	absorption					1010:1019	excellent water absorption	994:1019	excellent water absorption	994:1019	The CMCS/Gel-PEDOT hydrogels exhibited high porosity, excellent water absorption, improved thermal stability, and adequate biodegradability.					
36500418	0	12	theme	Conductive	72:81	arg1	Nanoparticles					89:101	Conductive PEDOT Nanoparticles	72:101	Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation	72:174	Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation.					
36500418	4	13	from	differentiation	853:867	arg1	hydrogels					911:919	conductive hydrogels	900:919	conductive hydrogels	900:919	The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated.					
36500418	1	14	theme	Tissue	177:182	arg1	engineering					184:194	Tissue engineering	177:194	Tissue engineering scaffolds	177:204	Tissue engineering scaffolds provide biological and physiochemical cures to guide tissue recovery, and electrical signals through the electroactive materials possess tremendous potential to modulate the cell fate.					
36500418	2	15	theme	carboxymethyl	535:547	arg1	surface					596:602	a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	533:602	a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	533:602	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	8	16	theme	neuronal	1570:1577	arg1	differentiation					1579:1593	neuronal differentiation	1570:1593	neuronal differentiation	1570:1593	In addition, under the differentiation condition, the conductive hydrogel also significantly enhanced neuronal differentiation with the up-regulation of β-tubulin III expression.					
36500418	5	17	theme	high	979:982	arg1	porosity					984:991	high porosity	979:991	high porosity	979:991	The CMCS/Gel-PEDOT hydrogels exhibited high porosity, excellent water absorption, improved thermal stability, and adequate biodegradability.					
36500418	2	18	theme	hydrogel	587:594	arg1	surface					596:602	a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	533:602	a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	533:602	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	0	19	theme	Improved	107:114	arg1	Cell					128:131	Improved Neural Stem Cell Proliferation and Neuronal Differentiation	107:174	Improved Neural Stem Cell Proliferation and Neuronal Differentiation	107:174	Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation.					
36500418	0	20	theme	Stem	123:126	arg1	Cell					128:131	Improved Neural Stem Cell Proliferation and Neuronal Differentiation	107:174	Improved Neural Stem Cell Proliferation and Neuronal Differentiation	107:174	Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation.					
36500418	5	21	theme	adequate	1054:1061	arg1	biodegradability					1063:1078	adequate biodegradability	1054:1078	adequate biodegradability	1054:1078	The CMCS/Gel-PEDOT hydrogels exhibited high porosity, excellent water absorption, improved thermal stability, and adequate biodegradability.					
36500418	2	22	from	nanoparticles	516:528	arg1	surface					596:602	a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	533:602	a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	533:602	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	2	23	theme	hydrogel	428:435	arg1	scaffold					437:444	a novel electroactive hydrogel scaffold	406:444	a novel electroactive hydrogel scaffold	406:444	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	0	24	theme	Carboxymethyl	0:12	arg1	Chitosan					14:21	Carboxymethyl Chitosan	0:21	Carboxymethyl Chitosan	0:21	Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation.					
36500418	7	25	theme	long-term	1382:1390	arg1	growth					1397:1402	long-term cell growth	1382:1402	long-term cell growth	1382:1402	Compared to the CMCS/Gel hydrogel, the incorporation of PEDOT nanoparticles significantly improved the adhesion of NSCs, and supported long-term cell growth and proliferation in a three-dimensional (3D) microenvironment.					
36500418	8	26	theme	expression	1635:1644	arg1	up-regulation					1604:1616	the up-regulation	1600:1616	the up-regulation of β-tubulin III expression	1600:1644	In addition, under the differentiation condition, the conductive hydrogel also significantly enhanced neuronal differentiation with the up-regulation of β-tubulin III expression.					
36500418	2	27	theme	poly	475:478	arg1	nanoparticles					516:528	poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles	475:528	poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	475:602	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	1	28	theme	tremendous	343:352	arg1	potential					354:362	tremendous potential	343:362	tremendous potential to modulate the cell fate	343:388	Tissue engineering scaffolds provide biological and physiochemical cures to guide tissue recovery, and electrical signals through the electroactive materials possess tremendous potential to modulate the cell fate.					
36500418	5	29	theme	thermal	1031:1037	arg1	stability					1039:1047	improved thermal stability	1022:1047	improved thermal stability	1022:1047	The CMCS/Gel-PEDOT hydrogels exhibited high porosity, excellent water absorption, improved thermal stability, and adequate biodegradability.					
36500418	6	30	theme	hydrogels	1136:1144	arg1	properties					1109:1118	the mechanical properties	1094:1118	the mechanical properties of the prepared hydrogels	1094:1144	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	6	30	theme	hydrogels	1136:1144	arg1	similar					1151:1157	similar	1151:1157	similar	1151:1157	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	4	31	theme	cells	884:888	arg1	proliferation					834:846	proliferation	834:846	proliferation	834:846	The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated.					
36500418	4	31	theme	cells	884:888	arg1	differentiation					853:867	differentiation	853:867	differentiation	853:867	The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated.					
36500418	4	31	theme	cells	884:888	arg1	adhesion					824:831	adhesion	824:831	adhesion	824:831	The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated.					
36500418	7	32	theme	NSCs	1362:1365	arg1	adhesion					1350:1357	the adhesion	1346:1357	the adhesion of NSCs	1346:1365	Compared to the CMCS/Gel hydrogel, the incorporation of PEDOT nanoparticles significantly improved the adhesion of NSCs, and supported long-term cell growth and proliferation in a three-dimensional (3D) microenvironment.					
36500418	3	33	theme	swelling	701:708	arg1	rate					710:713	swelling rate	701:713	swelling rate	701:713	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	9	34	theme	attractive	1709:1718	arg1	substrate					1731:1739	an attractive conductive substrate	1706:1739	an attractive conductive substrate for further studies on neural tissue repair and regeneration	1706:1800	These results suggest that CMCS/Gel-PEDOT hydrogels may be an attractive conductive substrate for further studies on neural tissue repair and regeneration.					
36500418	9	34	theme	attractive	1709:1718	arg1	hydrogels					1689:1697	CMCS/Gel-PEDOT hydrogels	1674:1697	CMCS/Gel-PEDOT hydrogels	1674:1697	These results suggest that CMCS/Gel-PEDOT hydrogels may be an attractive conductive substrate for further studies on neural tissue repair and regeneration.					
36500418	6	35	theme	electrical	1190:1199	arg1	up					1214:1215	electrical conductivity up	1190:1215	electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm	1190:1244	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	8	36	theme	differentiation	1491:1505	arg1	condition					1507:1515	the differentiation condition	1487:1515	the differentiation condition	1487:1515	In addition, under the differentiation condition, the conductive hydrogel also significantly enhanced neuronal differentiation with the up-regulation of β-tubulin III expression.					
36500418	2	37	theme	novel	408:412	arg1	scaffold					437:444	a novel electroactive hydrogel scaffold	406:444	a novel electroactive hydrogel scaffold	406:444	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	9	38	theme	further	1745:1751	arg1	studies					1753:1759	further studies	1745:1759	further studies on neural tissue repair and regeneration	1745:1800	These results suggest that CMCS/Gel-PEDOT hydrogels may be an attractive conductive substrate for further studies on neural tissue repair and regeneration.					
36500418	7	39	theme	nanoparticles	1309:1321	arg1	incorporation					1286:1298	the incorporation	1282:1298	the incorporation of PEDOT nanoparticles	1282:1321	Compared to the CMCS/Gel hydrogel, the incorporation of PEDOT nanoparticles significantly improved the adhesion of NSCs, and supported long-term cell growth and proliferation in a three-dimensional (3D) microenvironment.					
36500418	3	40	dep	in	716:717	arg1	vitro					719:723	vitro	719:723	vitro	719:723	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	6	41	theme	brain	1171:1175	arg1	tissue					1177:1182	brain tissue	1171:1182	brain tissue	1171:1182	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	3	42	theme	mechanical	745:754	arg1	properties					756:765	mechanical properties	745:765	mechanical properties	745:765	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	9	43	theme	tissue	1771:1776	arg1	repair					1778:1783	neural tissue repair	1764:1783	neural tissue repair	1764:1783	These results suggest that CMCS/Gel-PEDOT hydrogels may be an attractive conductive substrate for further studies on neural tissue repair and regeneration.					
36500418	6	44	theme	10-3	1236:1239	arg1	±					1226:1226	1.52 ± 0.15	1221:1231	1.52 ± 0.15	1221:1231	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	6	44	theme	10-3	1236:1239	arg1	S/cm					1241:1244	(1.52 ± 0.15) × 10-3 S/cm	1220:1244	(1.52 ± 0.15) × 10-3 S/cm	1220:1244	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	3	45	theme	samples	792:798	arg1	morphology					665:674	morphology	665:674	morphology	665:674	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	3	45	theme	samples	792:798	arg1	rate					710:713	swelling rate	701:713	swelling rate	701:713	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	3	45	theme	samples	792:798	arg1	properties					756:765	mechanical properties	745:765	mechanical properties	745:765	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	3	45	theme	samples	792:798	arg1	conductivity					677:688	conductivity	677:688	conductivity	677:688	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	3	45	theme	samples	792:798	arg1	porosity					691:698	porosity	691:698	porosity	691:698	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	3	45	theme	samples	792:798	arg1	biodegradation					725:738	in vitro biodegradation	716:738	in vitro biodegradation	716:738	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	3	45	theme	samples	792:798	arg1	structure					654:662	The chemical structure	641:662	The chemical structure	641:662	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	1	46	contain	possess	335:341	arg2	potential					354:362	tremendous potential	343:362	tremendous potential to modulate the cell fate	343:388	Tissue engineering scaffolds provide biological and physiochemical cures to guide tissue recovery, and electrical signals through the electroactive materials possess tremendous potential to modulate the cell fate.					
36500418	1	46	contain	possess	335:341	arg1	signals					291:297	electrical signals	280:297	electrical signals through the electroactive materials	280:333	Tissue engineering scaffolds provide biological and physiochemical cures to guide tissue recovery, and electrical signals through the electroactive materials possess tremendous potential to modulate the cell fate.					
36500418	7	47	dep	three-dimensional	1427:1443	arg1	3D					1446:1447	3D	1446:1447	3D	1446:1447	Compared to the CMCS/Gel hydrogel, the incorporation of PEDOT nanoparticles significantly improved the adhesion of NSCs, and supported long-term cell growth and proliferation in a three-dimensional (3D) microenvironment.					
36500418	2	48	dep	in	608:609	arg1	situ					611:614	situ	611:614	situ	611:614	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	3	49	theme	prepared	774:781	arg1	samples					792:798	the prepared hydrogel samples	770:798	the prepared hydrogel samples	770:798	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	4	50	theme	conductive	900:909	arg1	hydrogels					911:919	conductive hydrogels	900:919	conductive hydrogels	900:919	The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated.					
36500418	2	51	theme	in	608:609	arg1	polymerization					625:638	in situ chemical polymerization	608:638	in situ chemical polymerization	608:638	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	4	52	dep	adhesion	824:831	arg1	The					820:822	The	820:822	The	820:822	The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated.					
36500418	8	53	theme	conductive	1522:1531	arg1	hydrogel					1533:1540	the conductive hydrogel	1518:1540	the conductive hydrogel	1518:1540	In addition, under the differentiation condition, the conductive hydrogel also significantly enhanced neuronal differentiation with the up-regulation of β-tubulin III expression.					
36500418	3	54	theme	chemical	645:652	arg1	structure					654:662	The chemical structure	641:662	The chemical structure	641:662	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	9	55	from	studies	1753:1759	arg1	regeneration					1789:1800	regeneration	1789:1800	regeneration	1789:1800	These results suggest that CMCS/Gel-PEDOT hydrogels may be an attractive conductive substrate for further studies on neural tissue repair and regeneration.					
36500418	9	55	from	studies	1753:1759	arg1	repair					1778:1783	neural tissue repair	1764:1783	neural tissue repair	1764:1783	These results suggest that CMCS/Gel-PEDOT hydrogels may be an attractive conductive substrate for further studies on neural tissue repair and regeneration.					
36500418	5	56	theme	excellent	994:1002	arg1	absorption					1010:1019	excellent water absorption	994:1019	excellent water absorption	994:1019	The CMCS/Gel-PEDOT hydrogels exhibited high porosity, excellent water absorption, improved thermal stability, and adequate biodegradability.					
36500418	2	57	theme	chemical	616:623	arg1	polymerization					625:638	in situ chemical polymerization	608:638	in situ chemical polymerization	608:638	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	0	58	theme	PEDOT	83:87	arg1	Nanoparticles					89:101	Conductive PEDOT Nanoparticles	72:101	Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation	72:174	Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation.					
36500418	8	59	theme	β-tubulin	1621:1629	arg1	expression					1635:1644	β-tubulin III expression	1621:1644	β-tubulin III expression	1621:1644	In addition, under the differentiation condition, the conductive hydrogel also significantly enhanced neuronal differentiation with the up-regulation of β-tubulin III expression.					
36500418	5	60	theme	CMCS/Gel-PEDOT	944:957	arg1	hydrogels					959:967	The CMCS/Gel-PEDOT hydrogels	940:967	The CMCS/Gel-PEDOT hydrogels	940:967	The CMCS/Gel-PEDOT hydrogels exhibited high porosity, excellent water absorption, improved thermal stability, and adequate biodegradability.					
36500418	6	61	theme	mechanical	1098:1107	arg1	properties					1109:1118	the mechanical properties	1094:1118	the mechanical properties of the prepared hydrogels	1094:1144	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	6	61	theme	mechanical	1098:1107	arg1	similar					1151:1157	similar	1151:1157	similar	1151:1157	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	2	62	theme	chitosan/gelatin	549:564	arg1	surface					596:602	a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	533:602	a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	533:602	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	7	63	theme	CMCS/Gel	1263:1270	arg1	hydrogel					1272:1279	the CMCS/Gel hydrogel	1259:1279	the CMCS/Gel hydrogel	1259:1279	Compared to the CMCS/Gel hydrogel, the incorporation of PEDOT nanoparticles significantly improved the adhesion of NSCs, and supported long-term cell growth and proliferation in a three-dimensional (3D) microenvironment.					
36500418	1	64	theme	engineering	184:194	arg1	scaffolds					196:204	Tissue engineering scaffolds	177:204	Tissue engineering scaffolds	177:204	Tissue engineering scaffolds provide biological and physiochemical cures to guide tissue recovery, and electrical signals through the electroactive materials possess tremendous potential to modulate the cell fate.					
36500418	0	65	theme	Neural	116:121	arg1	Cell					128:131	Improved Neural Stem Cell Proliferation and Neuronal Differentiation	107:174	Improved Neural Stem Cell Proliferation and Neuronal Differentiation	107:174	Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation.					
36500418	4	66	from	proliferation	834:846	arg1	hydrogels					911:919	conductive hydrogels	900:919	conductive hydrogels	900:919	The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated.					
36500418	0	67	dep	Cell	128:131	arg1	Proliferation					133:145	Proliferation	133:145	Proliferation	133:145	Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation.					
36500418	0	67	dep	Cell	128:131	arg1	Differentiation					160:174	Neuronal Differentiation	151:174	Neuronal Differentiation	151:174	Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation.					
36500418	2	68	theme	composite	577:585	arg1	surface					596:602	a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	533:602	a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	533:602	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	4	69	from	adhesion	824:831	arg1	hydrogels					911:919	conductive hydrogels	900:919	conductive hydrogels	900:919	The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated.					
36500418	7	70	from	proliferation	1408:1420	arg1	microenvironment					1450:1465	a three-dimensional (3D) microenvironment	1425:1465	a three-dimensional (3D) microenvironment	1425:1465	Compared to the CMCS/Gel hydrogel, the incorporation of PEDOT nanoparticles significantly improved the adhesion of NSCs, and supported long-term cell growth and proliferation in a three-dimensional (3D) microenvironment.					
36500418	7	71	theme	cell	1392:1395	arg1	growth					1397:1402	long-term cell growth	1382:1402	long-term cell growth	1382:1402	Compared to the CMCS/Gel hydrogel, the incorporation of PEDOT nanoparticles significantly improved the adhesion of NSCs, and supported long-term cell growth and proliferation in a three-dimensional (3D) microenvironment.					
36500418	2	72	theme	CMCS/Gel	567:574	arg1	surface					596:602	a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	533:602	a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface	533:602	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	2	73	theme	electroactive	414:426	arg1	scaffold					437:444	a novel electroactive hydrogel scaffold	406:444	a novel electroactive hydrogel scaffold	406:444	In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization.					
36500418	0	74	theme	Gelatin	27:33	arg1	Hydrogel					35:42	Gelatin Hydrogel	27:42	Gelatin Hydrogel	27:42	Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation.					
36500418	4	75	theme	neural	872:877	arg1	cells					884:888	neural stem cells	872:888	neural stem cells (NSCs)	872:895	The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated.					
36500418	4	75	theme	neural	872:877	arg1	NSCs					891:894	NSCs	891:894	NSCs	891:894	The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated.					
36500418	5	76	theme	improved	1022:1029	arg1	stability					1039:1047	improved thermal stability	1022:1047	improved thermal stability	1022:1047	The CMCS/Gel-PEDOT hydrogels exhibited high porosity, excellent water absorption, improved thermal stability, and adequate biodegradability.					
36500418	6	77	with	similar	1151:1157	arg1	up					1214:1215	electrical conductivity up	1190:1215	electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm	1190:1244	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	6	78	theme	prepared	1127:1134	arg1	hydrogels					1136:1144	the prepared hydrogels	1123:1144	the prepared hydrogels	1123:1144	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	4	79	theme	stem	879:882	arg1	cells					884:888	neural stem cells	872:888	neural stem cells (NSCs)	872:895	The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated.					
36500418	4	79	theme	stem	879:882	arg1	NSCs					891:894	NSCs	891:894	NSCs	891:894	The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated.					
36500418	0	80	dep	Scaffolds	44:52	arg1	Incorporated					54:65	Incorporated	54:65	Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation	44:174	Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation.					
36500418	1	81	theme	cell	380:383	arg1	fate					385:388	the cell fate	376:388	the cell fate	376:388	Tissue engineering scaffolds provide biological and physiochemical cures to guide tissue recovery, and electrical signals through the electroactive materials possess tremendous potential to modulate the cell fate.					
36500418	9	82	theme	conductive	1720:1729	arg1	substrate					1731:1739	an attractive conductive substrate	1706:1739	an attractive conductive substrate for further studies on neural tissue repair and regeneration	1706:1800	These results suggest that CMCS/Gel-PEDOT hydrogels may be an attractive conductive substrate for further studies on neural tissue repair and regeneration.					
36500418	9	82	theme	conductive	1720:1729	arg1	hydrogels					1689:1697	CMCS/Gel-PEDOT hydrogels	1674:1697	CMCS/Gel-PEDOT hydrogels	1674:1697	These results suggest that CMCS/Gel-PEDOT hydrogels may be an attractive conductive substrate for further studies on neural tissue repair and regeneration.					
36500418	6	83	theme	conductivity	1201:1212	arg1	up					1214:1215	electrical conductivity up	1190:1215	electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm	1190:1244	Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10-3 S/cm.					
36500418	3	84	theme	in	716:717	arg1	biodegradation					725:738	in vitro biodegradation	716:738	in vitro biodegradation	716:738	The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized.					
36500418	7	85	theme	PEDOT	1303:1307	arg1	nanoparticles					1309:1321	PEDOT nanoparticles	1303:1321	PEDOT nanoparticles	1303:1321	Compared to the CMCS/Gel hydrogel, the incorporation of PEDOT nanoparticles significantly improved the adhesion of NSCs, and supported long-term cell growth and proliferation in a three-dimensional (3D) microenvironment.					
36500418	1	86	theme	electrical	280:289	arg1	signals					291:297	electrical signals	280:297	electrical signals through the electroactive materials	280:333	Tissue engineering scaffolds provide biological and physiochemical cures to guide tissue recovery, and electrical signals through the electroactive materials possess tremendous potential to modulate the cell fate.					
35698417	1	0	theme	air	156:158	arg1	materials					171:179	air filtration materials	156:179	air filtration materials	156:179	It is still a challenge to prepare air filtration materials with high filtration efficiency and good thermal stability from renewable materials.					
35698417	2	1	theme	double	422:427	arg1	filtration					429:438	double filtration	422:438	double filtration	422:438	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	1	2	theme	filtration	160:169	arg1	materials					171:179	air filtration materials	156:179	air filtration materials	156:179	It is still a challenge to prepare air filtration materials with high filtration efficiency and good thermal stability from renewable materials.					
35698417	6	3	theme	aerogel	1060:1066	arg1	sheet					1068:1072	the aerogel sheet	1056:1072	the aerogel sheet	1056:1072	The environmentally friendliness excellent filtration efficiency and simplistic fabrication make the aerogel sheet a potential material choice to cope with the severe air pollution today.					
35698417	3	4	theme	electrostatic	628:640	arg1	adsorption					642:651	electrostatic adsorption	628:651	electrostatic adsorption	628:651	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	6	5	theme	air	1126:1128	arg1	pollution					1130:1138	the severe air pollution	1115:1138	the severe air pollution	1115:1138	The environmentally friendliness excellent filtration efficiency and simplistic fabrication make the aerogel sheet a potential material choice to cope with the severe air pollution today.					
35698417	3	6	theme	aerogel	495:501	arg1	sheet					503:507	The resulting CNF/PVA/BAC aerogel sheet	469:507	The resulting CNF/PVA/BAC aerogel sheet	469:507	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	0	7	theme	thermal	102:108	arg1	stability					110:118	thermal stability	102:118	thermal stability	102:118	Cellulose nanofibril/PVA/bamboo activated charcoal aerogel sheet with excellent capture for PM2.5 and thermal stability.					
35698417	2	8	used	used	378:381	arg2	nanofibril					291:300	cellulose nanofibril	281:300	cellulose nanofibril (CNF)	281:306	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	2	8	used	used	378:381	arg2	CNF					303:305	CNF	303:305	CNF	303:305	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	2	8	used	used	378:381	arg2	alcohol					321:327	vinyl alcohol	315:327	vinyl alcohol	315:327	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	2	8	used	used	378:381	arg2	poly					309:312	poly	309:312	poly (vinyl alcohol) (PVA)	309:334	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	2	8	used	used	378:381	arg2	PVA					331:333	PVA	331:333	PVA	331:333	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	2	8	used	used	378:381	arg2	charcoal					358:365	bamboo activated charcoal	341:365	bamboo activated charcoal (BAC)	341:371	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	2	8	used	used	378:381	arg2	BAC					368:370	BAC	368:370	BAC	368:370	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	4	9	theme	thermal	701:707	arg1	stability					709:717	high thermal stability	696:717	high thermal stability with its filtration efficiency	696:748	The composite material also showed high thermal stability with its filtration efficiency over 95% even after exposure to 200 °C.					
35698417	2	10	theme	cellulose	281:289	arg1	nanofibril					291:300	cellulose nanofibril	281:300	cellulose nanofibril (CNF)	281:306	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	2	10	theme	cellulose	281:289	arg1	CNF					303:305	CNF	303:305	CNF	303:305	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	1	11	theme	high	186:189	arg1	efficiency					202:211	high filtration efficiency	186:211	high filtration efficiency	186:211	It is still a challenge to prepare air filtration materials with high filtration efficiency and good thermal stability from renewable materials.					
35698417	3	12	theme	BAC	656:658	arg1	adsorption					642:651	electrostatic adsorption	628:651	electrostatic adsorption	628:651	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	3	12	theme	BAC	656:658	arg1	CNF					620:622	CNF	620:622	CNF	620:622	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	6	13	theme	material	1086:1093	arg1	choice					1095:1100	a potential material choice	1074:1100	a potential material choice to cope with the severe air pollution today	1074:1144	The environmentally friendliness excellent filtration efficiency and simplistic fabrication make the aerogel sheet a potential material choice to cope with the severe air pollution today.					
35698417	3	14	theme	CNF/PVA/BAC	483:493	arg1	sheet					503:507	The resulting CNF/PVA/BAC aerogel sheet	469:507	The resulting CNF/PVA/BAC aerogel sheet	469:507	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	2	15	theme	activated	348:356	arg1	BAC					368:370	BAC	368:370	BAC	368:370	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	2	15	theme	activated	348:356	arg1	charcoal					358:365	bamboo activated charcoal	341:365	bamboo activated charcoal (BAC)	341:371	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	6	16	theme	severe	1119:1124	arg1	pollution					1130:1138	the severe air pollution	1115:1138	the severe air pollution	1115:1138	The environmentally friendliness excellent filtration efficiency and simplistic fabrication make the aerogel sheet a potential material choice to cope with the severe air pollution today.					
35698417	3	17	theme	filtration	519:528	arg1	efficiency					530:539	a filtration efficiency	517:539	a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC	517:658	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	6	18	theme	simplistic	1028:1037	arg1	fabrication					1039:1049	simplistic fabrication	1028:1049	simplistic fabrication	1028:1049	The environmentally friendliness excellent filtration efficiency and simplistic fabrication make the aerogel sheet a potential material choice to cope with the severe air pollution today.					
35698417	2	19	theme	bamboo	341:346	arg1	BAC					368:370	BAC	368:370	BAC	368:370	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	2	19	theme	bamboo	341:346	arg1	charcoal					358:365	bamboo activated charcoal	341:365	bamboo activated charcoal (BAC)	341:371	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	1	20	theme	filtration	191:200	arg1	efficiency					202:211	high filtration efficiency	186:211	high filtration efficiency	186:211	It is still a challenge to prepare air filtration materials with high filtration efficiency and good thermal stability from renewable materials.					
35698417	0	21	theme	charcoal	42:49	arg1	sheet					59:63	charcoal aerogel sheet	42:63	charcoal aerogel sheet	42:63	Cellulose nanofibril/PVA/bamboo activated charcoal aerogel sheet with excellent capture for PM2.5 and thermal stability.					
35698417	4	22	theme	high	696:699	arg1	stability					709:717	high thermal stability	696:717	high thermal stability with its filtration efficiency	696:748	The composite material also showed high thermal stability with its filtration efficiency over 95% even after exposure to 200 °C.					
35698417	4	23	with	stability	709:717	arg1	efficiency					739:748	its filtration efficiency	724:748	its filtration efficiency	724:748	The composite material also showed high thermal stability with its filtration efficiency over 95% even after exposure to 200 °C.					
35698417	3	24	theme	resulting	473:481	arg1	sheet					503:507	The resulting CNF/PVA/BAC aerogel sheet	469:507	The resulting CNF/PVA/BAC aerogel sheet	469:507	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	6	25	theme	filtration	1002:1011	arg1	efficiency					1013:1022	The environmentally friendliness excellent filtration efficiency	959:1022	The environmentally friendliness excellent filtration efficiency	959:1022	The environmentally friendliness excellent filtration efficiency and simplistic fabrication make the aerogel sheet a potential material choice to cope with the severe air pollution today.					
35698417	6	26	theme	excellent	992:1000	arg1	efficiency					1013:1022	The environmentally friendliness excellent filtration efficiency	959:1022	The environmentally friendliness excellent filtration efficiency	959:1022	The environmentally friendliness excellent filtration efficiency and simplistic fabrication make the aerogel sheet a potential material choice to cope with the severe air pollution today.					
35698417	3	27	theme	%	549:549	arg1	efficiency					530:539	a filtration efficiency	517:539	a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC	517:658	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	5	28	theme	hydrophobic	814:824	arg1	sheet					849:853	the hydrophobic CNF/PVA/BAC filtration sheet	810:853	the hydrophobic CNF/PVA/BAC filtration sheet	810:853	After modification, the hydrophobic CNF/PVA/BAC filtration sheet could be reused for over 5 times by water washing whereby the filtration efficiency remained above 95%.					
35698417	1	29	theme	good	217:220	arg1	stability					230:238	good thermal stability	217:238	good thermal stability	217:238	It is still a challenge to prepare air filtration materials with high filtration efficiency and good thermal stability from renewable materials.					
35698417	0	30	theme	aerogel	51:57	arg1	sheet					59:63	charcoal aerogel sheet	42:63	charcoal aerogel sheet	42:63	Cellulose nanofibril/PVA/bamboo activated charcoal aerogel sheet with excellent capture for PM2.5 and thermal stability.					
35698417	3	31	theme	double	572:577	arg1	filtration					579:588	its double filtration	568:588	its double filtration from the network structure of CNF and electrostatic adsorption of BAC	568:658	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	1	32	theme	thermal	222:228	arg1	stability					230:238	good thermal stability	217:238	good thermal stability	217:238	It is still a challenge to prepare air filtration materials with high filtration efficiency and good thermal stability from renewable materials.					
35698417	0	33	theme	excellent	70:78	arg1	capture					80:86	excellent capture	70:86	excellent capture for PM2.5 and thermal stability	70:118	Cellulose nanofibril/PVA/bamboo activated charcoal aerogel sheet with excellent capture for PM2.5 and thermal stability.					
35698417	3	34	from	structure	607:615	arg1	filtration					579:588	its double filtration	568:588	its double filtration from the network structure of CNF and electrostatic adsorption of BAC	568:658	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	2	35	theme	air	395:397	arg1	system					410:415	an air filtration system	392:415	an air filtration system	392:415	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	1	36	from	materials	255:263	arg1	stability					230:238	good thermal stability	217:238	good thermal stability	217:238	It is still a challenge to prepare air filtration materials with high filtration efficiency and good thermal stability from renewable materials.					
35698417	1	36	from	materials	255:263	arg1	efficiency					202:211	high filtration efficiency	186:211	high filtration efficiency	186:211	It is still a challenge to prepare air filtration materials with high filtration efficiency and good thermal stability from renewable materials.					
35698417	3	37	theme	CNF	620:622	arg1	structure					607:615	the network structure	595:615	the network structure of CNF and electrostatic adsorption of BAC	595:658	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	2	38	theme	vinyl	315:319	arg1	alcohol					321:327	vinyl alcohol	315:327	vinyl alcohol	315:327	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	2	38	theme	vinyl	315:319	arg1	poly					309:312	poly	309:312	poly (vinyl alcohol) (PVA)	309:334	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	5	39	theme	filtration	917:926	arg1	efficiency					928:937	the filtration efficiency	913:937	the filtration efficiency	913:937	After modification, the hydrophobic CNF/PVA/BAC filtration sheet could be reused for over 5 times by water washing whereby the filtration efficiency remained above 95%.					
35698417	3	40	theme	network	599:605	arg1	structure					607:615	the network structure	595:615	the network structure of CNF and electrostatic adsorption of BAC	595:658	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	4	41	theme	filtration	728:737	arg1	efficiency					739:748	its filtration efficiency	724:748	its filtration efficiency	724:748	The composite material also showed high thermal stability with its filtration efficiency over 95% even after exposure to 200 °C.					
35698417	6	42	theme	potential	1076:1084	arg1	choice					1095:1100	a potential material choice	1074:1100	a potential material choice to cope with the severe air pollution today	1074:1144	The environmentally friendliness excellent filtration efficiency and simplistic fabrication make the aerogel sheet a potential material choice to cope with the severe air pollution today.					
35698417	4	43	theme	composite	665:673	arg1	material					675:682	The composite material	661:682	The composite material	661:682	The composite material also showed high thermal stability with its filtration efficiency over 95% even after exposure to 200 °C.					
35698417	6	44	theme	friendliness	979:990	arg1	efficiency					1013:1022	The environmentally friendliness excellent filtration efficiency	959:1022	The environmentally friendliness excellent filtration efficiency	959:1022	The environmentally friendliness excellent filtration efficiency and simplistic fabrication make the aerogel sheet a potential material choice to cope with the severe air pollution today.					
35698417	5	45	theme	CNF/PVA/BAC	826:836	arg1	sheet					849:853	the hydrophobic CNF/PVA/BAC filtration sheet	810:853	the hydrophobic CNF/PVA/BAC filtration sheet	810:853	After modification, the hydrophobic CNF/PVA/BAC filtration sheet could be reused for over 5 times by water washing whereby the filtration efficiency remained above 95%.					
35698417	1	46	theme	renewable	245:253	arg1	materials					255:263	renewable materials	245:263	renewable materials	245:263	It is still a challenge to prepare air filtration materials with high filtration efficiency and good thermal stability from renewable materials.					
35698417	2	47	theme	filtration	399:408	arg1	system					410:415	an air filtration system	392:415	an air filtration system	392:415	In this study, cellulose nanofibril (CNF), poly (vinyl alcohol) (PVA), and bamboo activated charcoal (BAC) were used to build an air filtration system with double filtration by mixing and freeze-drying.					
35698417	3	48	theme	adsorption	642:651	arg1	structure					607:615	the network structure	595:615	the network structure of CNF and electrostatic adsorption of BAC	595:658	The resulting CNF/PVA/BAC aerogel sheet reached a filtration efficiency of 99.69% for PM2.5 due to its double filtration from the network structure of CNF and electrostatic adsorption of BAC.					
35698417	5	49	theme	filtration	838:847	arg1	sheet					849:853	the hydrophobic CNF/PVA/BAC filtration sheet	810:853	the hydrophobic CNF/PVA/BAC filtration sheet	810:853	After modification, the hydrophobic CNF/PVA/BAC filtration sheet could be reused for over 5 times by water washing whereby the filtration efficiency remained above 95%.					
35348168	2	0	theme	cellulose	513:521	arg1	support					529:535	a polypyrrole-coated bacterial cellulose (PBC) support	482:535	a polypyrrole-coated bacterial cellulose (PBC) support	482:535	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	3	1	theme	bases	713:717	arg1	environments					681:692	complex water environments	667:692	complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times	667:793	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	3	2	theme	heat	720:723	arg1	environments					681:692	complex water environments	667:692	complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times	667:793	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	2	3	theme	natural	354:360	arg1	structure					362:370	the natural structure	350:370	the natural structure of sandy soils	350:385	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	1	4	theme	aqueous	251:257	arg1	contaminants					259:270	complex aqueous contaminants	243:270	complex aqueous contaminants	243:270	The development of multifunctional films with a high permeability has been of great concern for effective separation of complex aqueous contaminants, especially in the face of zero or near-zero release regulations.					
35348168	0	5	theme	water	70:74	arg1	permeance					76:84	a superhigh water permeance	58:84	a superhigh water permeance through graded nanofluidic channels	58:120	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.					
35348168	2	6	theme	nanofluidic	608:618	arg1	channels					620:627	graded nanofluidic channels	601:627	graded nanofluidic channels	601:627	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	1	7	theme	contaminants	259:270	arg1	separation					229:238	effective separation	219:238	effective separation of complex aqueous contaminants	219:270	The development of multifunctional films with a high permeability has been of great concern for effective separation of complex aqueous contaminants, especially in the face of zero or near-zero release regulations.					
35348168	0	8	theme	graded	94:99	arg1	channels					113:120	graded nanofluidic channels	94:120	graded nanofluidic channels	94:120	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.					
35348168	3	9	theme	acids	706:710	arg1	environments					681:692	complex water environments	667:692	complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times	667:793	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	2	10	theme	graded	601:606	arg1	channels					620:627	graded nanofluidic channels	601:627	graded nanofluidic channels	601:627	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	3	11	dep	structure	800:808	arg1	the					796:798	the	796:798	the	796:798	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	6	12	theme	key	1445:1447	arg1	role					1449:1452	a key role	1443:1452	a key role for rendering such an outstandingly comprehensive performance of the film	1443:1526	Besides the superhydrophilicity and underwater superoleophobicity, the synapse-like-structure-induced graded nanofluidic channels are also proposed to play a key role for rendering such an outstandingly comprehensive performance of the film by greatly overcoming fluid resistance and reducing permeation viscosity.					
35348168	6	13	theme	underwater	1323:1332	arg1	superoleophobicity					1334:1351	the superhydrophilicity and underwater superoleophobicity	1295:1351	superoleophobicity	1334:1351	Besides the superhydrophilicity and underwater superoleophobicity, the synapse-like-structure-induced graded nanofluidic channels are also proposed to play a key role for rendering such an outstandingly comprehensive performance of the film by greatly overcoming fluid resistance and reducing permeation viscosity.					
35348168	1	14	with	films	158:162	arg1	permeability					176:187	a high permeability	169:187	a high permeability	169:187	The development of multifunctional films with a high permeability has been of great concern for effective separation of complex aqueous contaminants, especially in the face of zero or near-zero release regulations.					
35348168	4	15	theme	salinity	935:942	arg1	environment					944:954	a high salinity environment	928:954	a high salinity environment over seawater	928:968	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	4	16	theme	thousand	1095:1102	arg1	magnitudes					1132:1141	one or two magnitudes	1121:1141	one or two magnitudes higher than that of the related films reported in the literature	1121:1206	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	4	16	theme	thousand	1095:1102	arg1	bar-1					1114:1118	one thousand L h-1 m-2 bar-1	1091:1118	one thousand L h-1 m-2 bar-1	1091:1118	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	6	17	theme	fluid	1550:1554	arg1	resistance					1556:1565	fluid resistance	1550:1565	fluid resistance	1550:1565	Besides the superhydrophilicity and underwater superoleophobicity, the synapse-like-structure-induced graded nanofluidic channels are also proposed to play a key role for rendering such an outstandingly comprehensive performance of the film by greatly overcoming fluid resistance and reducing permeation viscosity.					
35348168	3	18	theme	high	877:880	arg1	stability					893:901	its high structural stability	873:901	its high structural stability	873:901	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	1	19	theme	high	171:174	arg1	permeability					176:187	a high permeability	169:187	a high permeability	169:187	The development of multifunctional films with a high permeability has been of great concern for effective separation of complex aqueous contaminants, especially in the face of zero or near-zero release regulations.					
35348168	0	20	theme	nanofluidic	101:111	arg1	channels					113:120	graded nanofluidic channels	94:120	graded nanofluidic channels	94:120	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.					
35348168	6	21	theme	superhydrophilicity	1299:1317	arg1	superoleophobicity					1334:1351	the superhydrophilicity and underwater superoleophobicity	1295:1351	superoleophobicity	1334:1351	Besides the superhydrophilicity and underwater superoleophobicity, the synapse-like-structure-induced graded nanofluidic channels are also proposed to play a key role for rendering such an outstandingly comprehensive performance of the film by greatly overcoming fluid resistance and reducing permeation viscosity.					
35348168	3	22	theme	else	753:756	arg1	environments					681:692	complex water environments	667:692	complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times	667:793	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	0	23	theme	film	9:12	arg1	separation					14:23	Absolute film separation	0:23	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.	0:121	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.					
35348168	2	24	theme	bacterial	503:511	arg1	support					529:535	a polypyrrole-coated bacterial cellulose (PBC) support	482:535	a polypyrrole-coated bacterial cellulose (PBC) support	482:535	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	2	25	theme	PBC	524:526	arg1	support					529:535	a polypyrrole-coated bacterial cellulose (PBC) support	482:535	a polypyrrole-coated bacterial cellulose (PBC) support	482:535	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	4	26	theme	L	1104:1104	arg1	magnitudes					1132:1141	one or two magnitudes	1121:1141	one or two magnitudes higher than that of the related films reported in the literature	1121:1206	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	4	26	theme	L	1104:1104	arg1	bar-1					1114:1118	one thousand L h-1 m-2 bar-1	1091:1118	one thousand L h-1 m-2 bar-1	1091:1118	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	2	27	theme	soils	381:385	arg1	structure					362:370	the natural structure	350:370	the natural structure of sandy soils	350:385	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	0	28	theme	Absolute	0:7	arg1	separation					14:23	Absolute film separation	0:23	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.	0:121	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.					
35348168	4	29	theme	water	1070:1074	arg1	permeance					1076:1084	a surprisingly superhigh water permeance	1045:1084	a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature	1045:1206	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	2	30	theme	sandy	375:379	arg1	soils					381:385	sandy soils	375:385	sandy soils	375:385	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	4	31	theme	dye-separation	1001:1014	arg1	capacity					1016:1023	a dye-separation capacity	999:1023	a dye-separation capacity of almost 100%	999:1038	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	2	32	theme	two-layered	553:563	arg1	film					584:587	a new two-layered PBC/PPSM composite film	547:587	a new two-layered PBC/PPSM composite film	547:587	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	4	33	theme	superhigh	1060:1068	arg1	permeance					1076:1084	a surprisingly superhigh water permeance	1045:1084	a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature	1045:1206	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	2	34	theme	new	549:551	arg1	film					584:587	a new two-layered PBC/PPSM composite film	547:587	a new two-layered PBC/PPSM composite film	547:587	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	3	35	theme	structural	882:891	arg1	stability					893:901	its high structural stability	873:901	its high structural stability	873:901	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	1	36	theme	great	201:205	arg1	concern					207:213	great concern	201:213	great concern	201:213	The development of multifunctional films with a high permeability has been of great concern for effective separation of complex aqueous contaminants, especially in the face of zero or near-zero release regulations.					
35348168	2	37	theme	polypyrrole-coated	484:501	arg1	support					529:535	a polypyrrole-coated bacterial cellulose (PBC) support	482:535	a polypyrrole-coated bacterial cellulose (PBC) support	482:535	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	3	38	theme	high	735:738	arg1	salinity					740:747	high salinity	735:747	high salinity	735:747	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	3	39	theme	complex	667:673	arg1	environments					681:692	complex water environments	667:692	complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times	667:793	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	6	40	theme	film	1523:1526	arg1	performance					1504:1514	such an outstandingly comprehensive performance	1468:1514	such an outstandingly comprehensive performance of the film	1468:1526	Besides the superhydrophilicity and underwater superoleophobicity, the synapse-like-structure-induced graded nanofluidic channels are also proposed to play a key role for rendering such an outstandingly comprehensive performance of the film by greatly overcoming fluid resistance and reducing permeation viscosity.					
35348168	2	41	theme	sub-micron	431:440	arg1	PPSM					451:454	PPSM	451:454	PPSM	451:454	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	2	41	theme	sub-micron	431:440	arg1	spheres					442:448	polydopamine-wrapped/connected polypyrrole sub-micron spheres	388:448	polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM)	388:455	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	0	42	theme	dyes/salts	28:37	arg1	separation					14:23	Absolute film separation	0:23	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.	0:121	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.					
35348168	5	43	theme	effective	1236:1244	arg1	oil-water-separation					1246:1265	effective oil-water-separation	1236:1265	effective oil-water-separation	1236:1265	Meanwhile, the ability for effective oil-water-separation was also validated.					
35348168	1	44	from	concern	207:213	arg1	face					291:294	the face	287:294	the face of zero or near-zero release regulations	287:335	The development of multifunctional films with a high permeability has been of great concern for effective separation of complex aqueous contaminants, especially in the face of zero or near-zero release regulations.					
35348168	2	45	theme	polypyrrole	419:429	arg1	PPSM					451:454	PPSM	451:454	PPSM	451:454	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	2	45	theme	polypyrrole	419:429	arg1	spheres					442:448	polydopamine-wrapped/connected polypyrrole sub-micron spheres	388:448	polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM)	388:455	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	4	46	theme	PBC/PPSM	976:983	arg1	film					985:988	this PBC/PPSM film	971:988	this PBC/PPSM film	971:988	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	6	47	theme	synapse-like-structure-induced	1358:1387	arg1	channels					1408:1415	the synapse-like-structure-induced graded nanofluidic channels	1354:1415	the synapse-like-structure-induced graded nanofluidic channels	1354:1415	Besides the superhydrophilicity and underwater superoleophobicity, the synapse-like-structure-induced graded nanofluidic channels are also proposed to play a key role for rendering such an outstandingly comprehensive performance of the film by greatly overcoming fluid resistance and reducing permeation viscosity.					
35348168	0	48	with	separation	14:23	arg1	permeance					76:84	a superhigh water permeance	58:84	a superhigh water permeance through graded nanofluidic channels	58:120	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.					
35348168	0	49	theme	emulsions	43:51	arg1	separation					14:23	Absolute film separation	0:23	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.	0:121	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.					
35348168	2	50	theme	polydopamine-wrapped/connected	388:417	arg1	PPSM					451:454	PPSM	451:454	PPSM	451:454	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	2	50	theme	polydopamine-wrapped/connected	388:417	arg1	spheres					442:448	polydopamine-wrapped/connected polypyrrole sub-micron spheres	388:448	polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM)	388:455	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	4	51	theme	higher	1143:1148	arg1	magnitudes					1132:1141	one or two magnitudes	1121:1141	one or two magnitudes higher than that of the related films reported in the literature	1121:1206	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	4	51	theme	higher	1143:1148	arg1	bar-1					1114:1118	one thousand L h-1 m-2 bar-1	1091:1118	one thousand L h-1 m-2 bar-1	1091:1118	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	6	52	theme	comprehensive	1490:1502	arg1	performance					1504:1514	such an outstandingly comprehensive performance	1468:1514	such an outstandingly comprehensive performance of the film	1468:1526	Besides the superhydrophilicity and underwater superoleophobicity, the synapse-like-structure-induced graded nanofluidic channels are also proposed to play a key role for rendering such an outstandingly comprehensive performance of the film by greatly overcoming fluid resistance and reducing permeation viscosity.					
35348168	1	53	theme	release	317:323	arg1	regulations					325:335	zero or near-zero release regulations	299:335	zero or near-zero release regulations	299:335	The development of multifunctional films with a high permeability has been of great concern for effective separation of complex aqueous contaminants, especially in the face of zero or near-zero release regulations.					
35348168	2	54	theme	composite	574:582	arg1	film					584:587	a new two-layered PBC/PPSM composite film	547:587	a new two-layered PBC/PPSM composite film	547:587	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	3	55	theme	ethanol	697:703	arg1	environments					681:692	complex water environments	667:692	complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times	667:793	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	4	56	theme	related	1167:1173	arg1	films					1175:1179	the related films	1163:1179	the related films reported in the literature	1163:1206	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	3	57	theme	film	834:837	arg1	performance					814:824	performance	814:824	performance	814:824	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	3	57	theme	film	834:837	arg1	structure					800:808	structure	800:808	structure	800:808	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	1	58	theme	effective	219:227	arg1	separation					229:238	effective separation	219:238	effective separation of complex aqueous contaminants	219:270	The development of multifunctional films with a high permeability has been of great concern for effective separation of complex aqueous contaminants, especially in the face of zero or near-zero release regulations.					
35348168	2	59	theme	PBC/PPSM	565:572	arg1	film					584:587	a new two-layered PBC/PPSM composite film	547:587	a new two-layered PBC/PPSM composite film	547:587	Inspired by the natural structure of sandy soils, polydopamine-wrapped/connected polypyrrole sub-micron spheres (PPSM) were closely packed onto a polypyrrole-coated bacterial cellulose (PBC) support, by which a new two-layered PBC/PPSM composite film formed with graded nanofluidic channels.					
35348168	3	60	theme	water	675:679	arg1	environments					681:692	complex water environments	667:692	complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times	667:793	Interestingly, after being soaked in complex water environments of ethanol, acids, bases, heat, cold and high salinity, or else bended/folded for more than 10 times, the structure and performance of this film still stayed the same, validating its high structural stability and flexibility.					
35348168	6	61	theme	graded	1389:1394	arg1	channels					1408:1415	the synapse-like-structure-induced graded nanofluidic channels	1354:1415	the synapse-like-structure-induced graded nanofluidic channels	1354:1415	Besides the superhydrophilicity and underwater superoleophobicity, the synapse-like-structure-induced graded nanofluidic channels are also proposed to play a key role for rendering such an outstandingly comprehensive performance of the film by greatly overcoming fluid resistance and reducing permeation viscosity.					
35348168	6	62	theme	permeation	1580:1589	arg1	viscosity					1591:1599	permeation viscosity	1580:1599	permeation viscosity	1580:1599	Besides the superhydrophilicity and underwater superoleophobicity, the synapse-like-structure-induced graded nanofluidic channels are also proposed to play a key role for rendering such an outstandingly comprehensive performance of the film by greatly overcoming fluid resistance and reducing permeation viscosity.					
35348168	4	63	theme	h-1	1106:1108	arg1	magnitudes					1132:1141	one or two magnitudes	1121:1141	one or two magnitudes higher than that of the related films reported in the literature	1121:1206	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	4	63	theme	h-1	1106:1108	arg1	bar-1					1114:1118	one thousand L h-1 m-2 bar-1	1091:1118	one thousand L h-1 m-2 bar-1	1091:1118	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	0	64	theme	superhigh	60:68	arg1	permeance					76:84	a superhigh water permeance	58:84	a superhigh water permeance through graded nanofluidic channels	58:120	Absolute film separation of dyes/salts and emulsions with a superhigh water permeance through graded nanofluidic channels.					
35348168	4	65	theme	m-2	1110:1112	arg1	magnitudes					1132:1141	one or two magnitudes	1121:1141	one or two magnitudes higher than that of the related films reported in the literature	1121:1206	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	4	65	theme	m-2	1110:1112	arg1	bar-1					1114:1118	one thousand L h-1 m-2 bar-1	1091:1118	one thousand L h-1 m-2 bar-1	1091:1118	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	1	66	theme	multifunctional	142:156	arg1	films					158:162	multifunctional films	142:162	multifunctional films with a high permeability	142:187	The development of multifunctional films with a high permeability has been of great concern for effective separation of complex aqueous contaminants, especially in the face of zero or near-zero release regulations.					
35348168	4	67	theme	%	1038:1038	arg1	capacity					1016:1023	a dye-separation capacity	999:1023	a dye-separation capacity of almost 100%	999:1038	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	4	68	theme	high	930:933	arg1	environment					944:954	a high salinity environment	928:954	a high salinity environment over seawater	928:968	Even in a high salinity environment over seawater, this PBC/PPSM film exhibits a dye-separation capacity of almost 100% with a surprisingly superhigh water permeance over one thousand L h-1 m-2 bar-1, one or two magnitudes higher than that of the related films reported in the literature.					
35348168	1	69	theme	regulations	325:335	arg1	face					291:294	the face	287:294	the face of zero or near-zero release regulations	287:335	The development of multifunctional films with a high permeability has been of great concern for effective separation of complex aqueous contaminants, especially in the face of zero or near-zero release regulations.					
35348168	6	70	theme	nanofluidic	1396:1406	arg1	channels					1408:1415	the synapse-like-structure-induced graded nanofluidic channels	1354:1415	the synapse-like-structure-induced graded nanofluidic channels	1354:1415	Besides the superhydrophilicity and underwater superoleophobicity, the synapse-like-structure-induced graded nanofluidic channels are also proposed to play a key role for rendering such an outstandingly comprehensive performance of the film by greatly overcoming fluid resistance and reducing permeation viscosity.					
35348168	1	71	theme	films	158:162	arg1	development					127:137	The development	123:137	The development of multifunctional films with a high permeability	123:187	The development of multifunctional films with a high permeability has been of great concern for effective separation of complex aqueous contaminants, especially in the face of zero or near-zero release regulations.					
35348168	1	72	theme	complex	243:249	arg1	contaminants					259:270	complex aqueous contaminants	243:270	complex aqueous contaminants	243:270	The development of multifunctional films with a high permeability has been of great concern for effective separation of complex aqueous contaminants, especially in the face of zero or near-zero release regulations.					
36080370	4	0	theme	method	777:782	arg1	optimization					784:795	method optimization	777:795	method optimization	777:795	For method optimization, an initial screening fractional factorial design was applied to identify the most significant parameters, followed by a face-centered central composite design to establish the optimal separation conditions.					
36080370	3	1	theme	charged	615:621	arg1	CDs					623:625	several neutral and charged CDs	595:625	several neutral and charged CDs	595:625	After an initial CS screening, using several neutral and charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), a neutral derivatized CD, was chosen as the optimum CS for the enantioseparation.					
36080370	4	2	theme	separation	982:991	arg1	conditions					993:1002	the optimal separation conditions	970:1002	the optimal separation conditions	970:1002	For method optimization, an initial screening fractional factorial design was applied to identify the most significant parameters, followed by a face-centered central composite design to establish the optimal separation conditions.					
36080370	8	3	theme	enantiomers	1578:1588	arg1	determination					1557:1569	the determination	1553:1569	the determination of MXL enantiomers in pharmaceuticals	1553:1607	The developed method was applied for the determination of MXL enantiomers in pharmaceuticals.					
36080370	5	4	theme	mM	1135:1136	arg1	buffer					1116:1121	60 mM phosphate buffer	1100:1121	60 mM phosphate buffer	1100:1121	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	4	theme	mM	1135:1136	arg1	TM-β-CD					1138:1144	50 mM TM-β-CD	1132:1144	50 mM TM-β-CD	1132:1144	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	9	5	theme	chiral	1693:1698	arg1	recognition					1700:1710	host-guest chiral recognition	1682:1710	host-guest chiral recognition	1682:1710	A computer modeling of the MXL-CD complexes was applied to characterize host-guest chiral recognition.					
36080370	1	6	theme	class	184:188	arg1	Mexiletine					162:171	Mexiletine	162:171	Mexiletine (MXL)	162:177	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	1	6	theme	class	184:188	arg1	agent					208:212	a class IB antiarrhythmic agent	182:212	a class IB antiarrhythmic agent	182:212	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	1	7	theme	antiarrhythmic	193:206	arg1	Mexiletine					162:171	Mexiletine	162:171	Mexiletine (MXL)	162:177	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	1	7	theme	antiarrhythmic	193:206	arg1	agent					208:212	a class IB antiarrhythmic agent	182:212	a class IB antiarrhythmic agent	182:212	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	3	8	theme	neutral	693:699	arg1	CD					713:714	a neutral derivatized CD	691:714	a neutral derivatized CD	691:714	After an initial CS screening, using several neutral and charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), a neutral derivatized CD, was chosen as the optimum CS for the enantioseparation.					
36080370	3	8	theme	neutral	693:699	arg1	heptakis-2,3,6-tri-O-methyl-β-CD					647:678	heptakis-2,3,6-tri-O-methyl-β-CD	647:678	heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD)	647:688	After an initial CS screening, using several neutral and charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), a neutral derivatized CD, was chosen as the optimum CS for the enantioseparation.					
36080370	6	9	theme	MXL	1223:1225	arg1	enantiomers					1227:1237	MXL enantiomers	1223:1237	MXL enantiomers	1223:1237	MXL enantiomers were baseline separated with a resolution of 1.52 during a migration time of under 5 min; S-MXL was the first migrating enantiomer.					
36080370	5	10	theme	best	1009:1012	arg1	results					1014:1020	The best results	1005:1020	The best results	1005:1020	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	6	11	theme	min	1324:1326	arg1	time					1308:1311	a migration time	1296:1311	a migration time of under 5 min	1296:1326	MXL enantiomers were baseline separated with a resolution of 1.52 during a migration time of under 5 min; S-MXL was the first migrating enantiomer.					
36080370	7	12	theme	Plackett-Burman	1491:1505	arg1	design					1507:1512	a Plackett-Burman design	1489:1512	a Plackett-Burman design	1489:1512	The method's analytical performance was verified in terms of precision, linearity, accuracy, and robustness (applying a Plackett-Burman design).					
36080370	4	13	theme	initial	801:807	arg1	screening					809:817	an initial screening	798:817	an initial screening fractional factorial design	798:845	For method optimization, an initial screening fractional factorial design was applied to identify the most significant parameters, followed by a face-centered central composite design to establish the optimal separation conditions.					
36080370	4	14	theme	fractional	819:828	arg1	design					840:845	an initial screening fractional factorial design	798:845	an initial screening fractional factorial design	798:845	For method optimization, an initial screening fractional factorial design was applied to identify the most significant parameters, followed by a face-centered central composite design to establish the optimal separation conditions.					
36080370	9	15	theme	computer	1612:1619	arg1	modeling					1621:1628	A computer modeling	1610:1628	A computer modeling of the MXL-CD complexes	1610:1652	A computer modeling of the MXL-CD complexes was applied to characterize host-guest chiral recognition.					
36080370	2	16	theme	MXL	454:456	arg1	enantiomers					458:468	MXL enantiomers	454:468	MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs)	454:555	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	7	17	theme	robustness	1468:1477	arg1	terms					1423:1427	terms	1423:1427	terms of precision, linearity, accuracy, and robustness (applying a Plackett-Burman design)	1423:1513	The method's analytical performance was verified in terms of precision, linearity, accuracy, and robustness (applying a Plackett-Burman design).					
36080370	2	18	theme	enantiomers	458:468	arg1	separation					440:449	the chiral separation	429:449	the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs)	429:555	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	5	19	theme	hydrodynamic	1189:1200	arg1	mbar/s					1215:1220	hydrodynamic injection 50 mbar/s	1189:1220	hydrodynamic injection 50 mbar/s	1189:1220	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	19	theme	hydrodynamic	1189:1200	arg1	buffer					1116:1121	60 mM phosphate buffer	1100:1121	60 mM phosphate buffer	1100:1121	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	1	20	theme	S-MXL	318:322	arg1	mixture					308:314	a racemic mixture R,S-MXL hydrochloride	298:336	mixture	308:314	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	1	20	theme	S-MXL	318:322	arg1	hydrochloride					324:336	S-MXL hydrochloride	318:336	S-MXL hydrochloride	318:336	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	9	21	theme	MXL-CD	1637:1642	arg1	complexes					1644:1652	the MXL-CD complexes	1633:1652	the MXL-CD complexes	1633:1652	A computer modeling of the MXL-CD complexes was applied to characterize host-guest chiral recognition.					
36080370	3	22	theme	optimum	735:741	arg1	heptakis-2,3,6-tri-O-methyl-β-CD					647:678	heptakis-2,3,6-tri-O-methyl-β-CD	647:678	heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD)	647:688	After an initial CS screening, using several neutral and charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), a neutral derivatized CD, was chosen as the optimum CS for the enantioseparation.					
36080370	3	22	theme	optimum	735:741	arg1	CS					743:744	the optimum CS	731:744	the optimum CS for the enantioseparation	731:770	After an initial CS screening, using several neutral and charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), a neutral derivatized CD, was chosen as the optimum CS for the enantioseparation.					
36080370	5	23	theme	optimized	1062:1070	arg1	conditions					1088:1097	the following optimized electrophoretic conditions	1048:1097	the following optimized electrophoretic conditions	1048:1097	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	6	24	dep	separated	1253:1261	arg1	baseline					1244:1251	baseline	1244:1251	baseline	1244:1251	MXL enantiomers were baseline separated with a resolution of 1.52 during a migration time of under 5 min; S-MXL was the first migrating enantiomer.					
36080370	5	25	theme	voltage	1174:1180	arg1	kV					1185:1186	applied voltage 30 kV	1166:1186	applied voltage 30 kV	1166:1186	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	25	theme	voltage	1174:1180	arg1	buffer					1116:1121	60 mM phosphate buffer	1100:1121	60 mM phosphate buffer	1100:1121	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	2	26	theme	capillary	476:484	arg1	CE					503:504	CE	503:504	CE	503:504	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	2	26	theme	capillary	476:484	arg1	cyclodextrins					511:523	cyclodextrins	511:523	cyclodextrins (CDs)	511:529	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	2	26	theme	capillary	476:484	arg1	electrophoresis					486:500	capillary electrophoresis	476:500	capillary electrophoresis (CE)	476:505	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	2	26	theme	capillary	476:484	arg1	selectors					541:549	chiral selectors	534:549	chiral selectors (CSs)	534:555	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	0	27	theme	Experimental	121:132	arg1	Method					141:146	Experimental Design Method	121:146	Experimental Design Method	121:146	Chiral Discrimination of Mexiletine Enantiomers by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors and Experimental Design Method Optimization.					
36080370	0	28	dep	Selectors	107:115	arg1	Optimization					148:159	Optimization	148:159	Optimization	148:159	Chiral Discrimination of Mexiletine Enantiomers by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors and Experimental Design Method Optimization.					
36080370	2	29	theme	efficient	408:416	arg1	method					418:423	a new, fast, and efficient method	391:423	a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs)	391:555	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	1	30	dep	mixture	308:314	arg1	R					316:316	R	316:316	R	316:316	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	0	31	theme	Chiral	0:5	arg1	Discrimination					7:20	Chiral Discrimination	0:20	Chiral Discrimination of Mexiletine Enantiomers by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors and Experimental Design Method Optimization.	0:160	Chiral Discrimination of Mexiletine Enantiomers by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors and Experimental Design Method Optimization.					
36080370	5	32	theme	phosphate	1106:1114	arg1	°C					1162:1163	temperature 20 °C	1147:1163	temperature 20 °C	1147:1163	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	32	theme	phosphate	1106:1114	arg1	buffer					1116:1121	60 mM phosphate buffer	1100:1121	60 mM phosphate buffer	1100:1121	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	32	theme	phosphate	1106:1114	arg1	TM-β-CD					1138:1144	50 mM TM-β-CD	1132:1144	50 mM TM-β-CD	1132:1144	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	32	theme	phosphate	1106:1114	arg1	pH					1124:1125	pH 5.0	1124:1129	pH 5.0	1124:1129	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	32	theme	phosphate	1106:1114	arg1	mbar/s					1215:1220	hydrodynamic injection 50 mbar/s	1189:1220	hydrodynamic injection 50 mbar/s	1189:1220	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	32	theme	phosphate	1106:1114	arg1	kV					1185:1186	applied voltage 30 kV	1166:1186	applied voltage 30 kV	1166:1186	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	2	33	theme	chiral	433:438	arg1	separation					440:449	the chiral separation	429:449	the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs)	429:555	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	8	34	from	pharmaceuticals	1593:1607	arg1	determination					1557:1569	the determination	1553:1569	the determination of MXL enantiomers in pharmaceuticals	1553:1607	The developed method was applied for the determination of MXL enantiomers in pharmaceuticals.					
36080370	0	35	theme	Enantiomers	36:46	arg1	Discrimination					7:20	Chiral Discrimination	0:20	Chiral Discrimination of Mexiletine Enantiomers by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors and Experimental Design Method Optimization.	0:160	Chiral Discrimination of Mexiletine Enantiomers by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors and Experimental Design Method Optimization.					
36080370	1	36	theme	racemic	300:306	arg1	mixture					308:314	a racemic mixture R,S-MXL hydrochloride	298:336	mixture	308:314	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	1	36	theme	racemic	300:306	arg1	hydrochloride					324:336	S-MXL hydrochloride	318:336	S-MXL hydrochloride	318:336	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	4	37	theme	central	932:938	arg1	design					950:955	a face-centered central composite design	916:955	a face-centered central composite design	916:955	For method optimization, an initial screening fractional factorial design was applied to identify the most significant parameters, followed by a face-centered central composite design to establish the optimal separation conditions.					
36080370	3	38	theme	CS	575:576	arg1	screening					578:586	an initial CS screening	564:586	an initial CS screening	564:586	After an initial CS screening, using several neutral and charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), a neutral derivatized CD, was chosen as the optimum CS for the enantioseparation.					
36080370	8	39	from	enantiomers	1578:1588	arg1	pharmaceuticals					1593:1607	pharmaceuticals	1593:1607	pharmaceuticals	1593:1607	The developed method was applied for the determination of MXL enantiomers in pharmaceuticals.					
36080370	6	40	theme	migration	1298:1306	arg1	time					1308:1311	a migration time	1296:1311	a migration time of under 5 min	1296:1326	MXL enantiomers were baseline separated with a resolution of 1.52 during a migration time of under 5 min; S-MXL was the first migrating enantiomer.					
36080370	5	41	theme	60	1100:1101	arg1	mM					1103:1104	mM	1103:1104	mM	1103:1104	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	2	42	theme	method	418:423	arg1	development					376:386	the development	372:386	the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs)	372:555	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	2	42	theme	method	418:423	arg1	aim					343:345	The aim	339:345	The aim of the current study	339:366	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	8	43	theme	developed	1520:1528	arg1	method					1530:1535	The developed method	1516:1535	The developed method	1516:1535	The developed method was applied for the determination of MXL enantiomers in pharmaceuticals.					
36080370	3	44	theme	several	595:601	arg1	CDs					623:625	several neutral and charged CDs	595:625	several neutral and charged CDs	595:625	After an initial CS screening, using several neutral and charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), a neutral derivatized CD, was chosen as the optimum CS for the enantioseparation.					
36080370	2	45	theme	current	354:360	arg1	study					362:366	the current study	350:366	the current study	350:366	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	5	46	theme	temperature	1147:1157	arg1	buffer					1116:1121	60 mM phosphate buffer	1100:1121	60 mM phosphate buffer	1100:1121	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	46	theme	temperature	1147:1157	arg1	°C					1162:1163	temperature 20 °C	1147:1163	temperature 20 °C	1147:1163	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	4	47	theme	optimal	974:980	arg1	conditions					993:1002	the optimal separation conditions	970:1002	the optimal separation conditions	970:1002	For method optimization, an initial screening fractional factorial design was applied to identify the most significant parameters, followed by a face-centered central composite design to establish the optimal separation conditions.					
36080370	6	48	theme	1.52	1284:1287	arg1	resolution					1270:1279	a resolution	1268:1279	a resolution of 1.52	1268:1287	MXL enantiomers were baseline separated with a resolution of 1.52 during a migration time of under 5 min; S-MXL was the first migrating enantiomer.					
36080370	6	49	theme	first	1343:1347	arg1	enantiomer					1359:1368	the first migrating enantiomer	1339:1368	the first migrating enantiomer	1339:1368	MXL enantiomers were baseline separated with a resolution of 1.52 during a migration time of under 5 min; S-MXL was the first migrating enantiomer.					
36080370	6	49	theme	first	1343:1347	arg1	S-MXL					1329:1333	S-MXL	1329:1333	S-MXL	1329:1333	MXL enantiomers were baseline separated with a resolution of 1.52 during a migration time of under 5 min; S-MXL was the first migrating enantiomer.					
36080370	1	50	theme	channel	262:268	arg1	blocker					270:276	a non-selective voltage-gated sodium channel blocker	225:276	a non-selective voltage-gated sodium channel blocker	225:276	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	5	51	theme	50	1132:1133	arg1	mM					1135:1136	mM	1135:1136	mM	1135:1136	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	2	52	theme	chiral	534:539	arg1	cyclodextrins					511:523	cyclodextrins	511:523	cyclodextrins (CDs)	511:529	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	2	52	theme	chiral	534:539	arg1	CSs					552:554	CSs	552:554	CSs	552:554	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	2	52	theme	chiral	534:539	arg1	electrophoresis					486:500	capillary electrophoresis	476:500	capillary electrophoresis (CE)	476:505	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	2	52	theme	chiral	534:539	arg1	selectors					541:549	chiral selectors	534:549	chiral selectors (CSs)	534:555	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	8	53	theme	MXL	1574:1576	arg1	enantiomers					1578:1588	MXL enantiomers	1574:1588	MXL enantiomers in pharmaceuticals	1574:1607	The developed method was applied for the determination of MXL enantiomers in pharmaceuticals.					
36080370	0	54	theme	Capillary	51:59	arg1	Electrophoresis					61:75	Capillary Electrophoresis	51:75	Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors and Experimental Design Method Optimization	51:159	Chiral Discrimination of Mexiletine Enantiomers by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors and Experimental Design Method Optimization.					
36080370	3	55	theme	pH	636:637	arg1	levels					639:644	four pH levels	631:644	four pH levels	631:644	After an initial CS screening, using several neutral and charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), a neutral derivatized CD, was chosen as the optimum CS for the enantioseparation.					
36080370	1	56	theme	IB	190:191	arg1	Mexiletine					162:171	Mexiletine	162:171	Mexiletine (MXL)	162:177	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	1	56	theme	IB	190:191	arg1	agent					208:212	a class IB antiarrhythmic agent	182:212	a class IB antiarrhythmic agent	182:212	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	4	57	theme	factorial	830:838	arg1	design					840:845	an initial screening fractional factorial design	798:845	an initial screening fractional factorial design	798:845	For method optimization, an initial screening fractional factorial design was applied to identify the most significant parameters, followed by a face-centered central composite design to establish the optimal separation conditions.					
36080370	3	58	theme	derivatized	701:711	arg1	CD					713:714	a neutral derivatized CD	691:714	a neutral derivatized CD	691:714	After an initial CS screening, using several neutral and charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), a neutral derivatized CD, was chosen as the optimum CS for the enantioseparation.					
36080370	3	58	theme	derivatized	701:711	arg1	heptakis-2,3,6-tri-O-methyl-β-CD					647:678	heptakis-2,3,6-tri-O-methyl-β-CD	647:678	heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD)	647:688	After an initial CS screening, using several neutral and charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), a neutral derivatized CD, was chosen as the optimum CS for the enantioseparation.					
36080370	4	59	theme	screening	809:817	arg1	design					840:845	an initial screening fractional factorial design	798:845	an initial screening fractional factorial design	798:845	For method optimization, an initial screening fractional factorial design was applied to identify the most significant parameters, followed by a face-centered central composite design to establish the optimal separation conditions.					
36080370	7	60	theme	accuracy	1454:1461	arg1	terms					1423:1427	terms	1423:1427	terms of precision, linearity, accuracy, and robustness (applying a Plackett-Burman design)	1423:1513	The method's analytical performance was verified in terms of precision, linearity, accuracy, and robustness (applying a Plackett-Burman design).					
36080370	4	61	theme	significant	880:890	arg1	parameters					892:901	the most significant parameters	871:901	the most significant parameters	871:901	For method optimization, an initial screening fractional factorial design was applied to identify the most significant parameters, followed by a face-centered central composite design to establish the optimal separation conditions.					
36080370	0	62	theme	Chiral	100:105	arg1	Selectors					107:115	Chiral Selectors	100:115	Chiral Selectors	100:115	Chiral Discrimination of Mexiletine Enantiomers by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors and Experimental Design Method Optimization.					
36080370	5	63	theme	following	1052:1060	arg1	conditions					1088:1097	the following optimized electrophoretic conditions	1048:1097	the following optimized electrophoretic conditions	1048:1097	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	7	64	theme	linearity	1443:1451	arg1	terms					1423:1427	terms	1423:1427	terms of precision, linearity, accuracy, and robustness (applying a Plackett-Burman design)	1423:1513	The method's analytical performance was verified in terms of precision, linearity, accuracy, and robustness (applying a Plackett-Burman design).					
36080370	9	65	theme	complexes	1644:1652	arg1	modeling					1621:1628	A computer modeling	1610:1628	A computer modeling of the MXL-CD complexes	1610:1652	A computer modeling of the MXL-CD complexes was applied to characterize host-guest chiral recognition.					
36080370	5	66	theme	applied	1166:1172	arg1	kV					1185:1186	applied voltage 30 kV	1166:1186	applied voltage 30 kV	1166:1186	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	66	theme	applied	1166:1172	arg1	buffer					1116:1121	60 mM phosphate buffer	1100:1121	60 mM phosphate buffer	1100:1121	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	67	theme	electrophoretic	1072:1086	arg1	conditions					1088:1097	the following optimized electrophoretic conditions	1048:1097	the following optimized electrophoretic conditions	1048:1097	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	7	68	theme	precision	1432:1440	arg1	terms					1423:1427	terms	1423:1427	terms of precision, linearity, accuracy, and robustness (applying a Plackett-Burman design)	1423:1513	The method's analytical performance was verified in terms of precision, linearity, accuracy, and robustness (applying a Plackett-Burman design).					
36080370	0	69	theme	Design	134:139	arg1	Method					141:146	Experimental Design Method	121:146	Experimental Design Method	121:146	Chiral Discrimination of Mexiletine Enantiomers by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors and Experimental Design Method Optimization.					
36080370	4	70	theme	composite	940:948	arg1	design					950:955	a face-centered central composite design	916:955	a face-centered central composite design	916:955	For method optimization, an initial screening fractional factorial design was applied to identify the most significant parameters, followed by a face-centered central composite design to establish the optimal separation conditions.					
36080370	8	71	from	determination	1557:1569	arg1	pharmaceuticals					1593:1607	pharmaceuticals	1593:1607	pharmaceuticals	1593:1607	The developed method was applied for the determination of MXL enantiomers in pharmaceuticals.					
36080370	5	72	theme	injection	1202:1210	arg1	mbar/s					1215:1220	hydrodynamic injection 50 mbar/s	1189:1220	hydrodynamic injection 50 mbar/s	1189:1220	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	72	theme	injection	1202:1210	arg1	buffer					1116:1121	60 mM phosphate buffer	1100:1121	60 mM phosphate buffer	1100:1121	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	2	73	theme	study	362:366	arg1	development					376:386	the development	372:386	the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs)	372:555	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	2	73	theme	study	362:366	arg1	aim					343:345	The aim	339:345	The aim of the current study	339:366	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	0	74	theme	Mexiletine	25:34	arg1	Enantiomers					36:46	Mexiletine Enantiomers	25:46	Mexiletine Enantiomers	25:46	Chiral Discrimination of Mexiletine Enantiomers by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors and Experimental Design Method Optimization.					
36080370	2	75	theme	fast	398:401	arg1	method					418:423	a new, fast, and efficient method	391:423	a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs)	391:555	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	2	76	theme	new	393:395	arg1	method					418:423	a new, fast, and efficient method	391:423	a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs)	391:555	The aim of the current study was the development of a new, fast, and efficient method for the chiral separation of MXL enantiomers using capillary electrophoresis (CE) and cyclodextrins (CDs) as chiral selectors (CSs).					
36080370	3	77	theme	initial	567:573	arg1	screening					578:586	an initial CS screening	564:586	an initial CS screening	564:586	After an initial CS screening, using several neutral and charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), a neutral derivatized CD, was chosen as the optimum CS for the enantioseparation.					
36080370	1	78	theme	voltage-gated	241:253	arg1	blocker					270:276	a non-selective voltage-gated sodium channel blocker	225:276	a non-selective voltage-gated sodium channel blocker	225:276	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	4	79	theme	face-centered	918:930	arg1	design					950:955	a face-centered central composite design	916:955	a face-centered central composite design	916:955	For method optimization, an initial screening fractional factorial design was applied to identify the most significant parameters, followed by a face-centered central composite design to establish the optimal separation conditions.					
36080370	3	80	theme	neutral	603:609	arg1	CDs					623:625	several neutral and charged CDs	595:625	several neutral and charged CDs	595:625	After an initial CS screening, using several neutral and charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), a neutral derivatized CD, was chosen as the optimum CS for the enantioseparation.					
36080370	5	81	theme	mM	1103:1104	arg1	°C					1162:1163	temperature 20 °C	1147:1163	temperature 20 °C	1147:1163	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	81	theme	mM	1103:1104	arg1	buffer					1116:1121	60 mM phosphate buffer	1100:1121	60 mM phosphate buffer	1100:1121	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	81	theme	mM	1103:1104	arg1	TM-β-CD					1138:1144	50 mM TM-β-CD	1132:1144	50 mM TM-β-CD	1132:1144	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	81	theme	mM	1103:1104	arg1	pH					1124:1125	pH 5.0	1124:1129	pH 5.0	1124:1129	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	81	theme	mM	1103:1104	arg1	mbar/s					1215:1220	hydrodynamic injection 50 mbar/s	1189:1220	hydrodynamic injection 50 mbar/s	1189:1220	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	5	81	theme	mM	1103:1104	arg1	kV					1185:1186	applied voltage 30 kV	1166:1186	applied voltage 30 kV	1166:1186	The best results were obtained by applying the following optimized electrophoretic conditions: 60 mM phosphate buffer, pH 5.0, 50 mM TM-β-CD, temperature 20 °C, applied voltage 30 kV, hydrodynamic injection 50 mbar/s.					
36080370	1	82	theme	non-selective	227:239	arg1	blocker					270:276	a non-selective voltage-gated sodium channel blocker	225:276	a non-selective voltage-gated sodium channel blocker	225:276	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36080370	7	83	theme	analytical	1384:1393	arg1	performance					1395:1405	The method's analytical performance	1371:1405	The method's analytical performance	1371:1405	The method's analytical performance was verified in terms of precision, linearity, accuracy, and robustness (applying a Plackett-Burman design).					
36080370	9	84	theme	host-guest	1682:1691	arg1	recognition					1700:1710	host-guest chiral recognition	1682:1710	host-guest chiral recognition	1682:1710	A computer modeling of the MXL-CD complexes was applied to characterize host-guest chiral recognition.					
36080370	6	85	theme	migrating	1349:1357	arg1	enantiomer					1359:1368	the first migrating enantiomer	1339:1368	the first migrating enantiomer	1339:1368	MXL enantiomers were baseline separated with a resolution of 1.52 during a migration time of under 5 min; S-MXL was the first migrating enantiomer.					
36080370	6	85	theme	migrating	1349:1357	arg1	S-MXL					1329:1333	S-MXL	1329:1333	S-MXL	1329:1333	MXL enantiomers were baseline separated with a resolution of 1.52 during a migration time of under 5 min; S-MXL was the first migrating enantiomer.					
36080370	1	86	theme	sodium	255:260	arg1	blocker					270:276	a non-selective voltage-gated sodium channel blocker	225:276	a non-selective voltage-gated sodium channel blocker	225:276	Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as a non-selective voltage-gated sodium channel blocker, used in therapy as a racemic mixture R,S-MXL hydrochloride.					
36126446	6	0	theme	NAAT-mediated	1008:1020	arg1	workflows					1042:1050	NAAT-mediated molecular diagnosis workflows	1008:1050	NAAT-mediated molecular diagnosis workflows in resource-constrained settings	1008:1083	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	4	1	theme	such	608:611	arg1	techniques					624:633	such extraction techniques	608:633	such extraction techniques	608:633	However, such extraction techniques traditionally utilize spin-column techniques that in turn require centralized high-speed centrifuges.					
36126446	3	2	from	extraction	534:543	arg1	sample					591:596	the clinical sample	578:596	the clinical sample	578:596	Similarly, genetic disease screening is also reliant on the successful extraction of pure patient genomic DNA from the clinical sample.					
36126446	12	3	from	difference	2099:2108	arg1	quality					2117:2123	the quality	2113:2123	the quality of the extracted DNA	2113:2144	Overall, our method demonstrated that DNA extraction could be performed utilizing toothpick-mounted cotton both with or without using a chaotropic salt, albeit with a difference in the quality of the extracted DNA.					
36126446	9	4	theme	study	1604:1608	arg1	validation					1586:1595	preliminary validation	1574:1595	preliminary validation of the study	1574:1608	The quality of the extracted DNA was then probed using PCR followed by agarose gel analysis for preliminary validation of the study.					
36126446	10	5	dep	efficiency	1679:1688	arg1	depending					1701:1709	depending	1701:1709	depending on serum composition	1701:1730	The qPCR experiments then quantitatively established the extraction efficiency (0.3-27 %, depending on serum composition).					
36126446	6	6	theme	extraction	891:900	arg1	methods					902:908	instrument-free nucleic acid extraction methods	862:908	instrument-free nucleic acid extraction methods	862:908	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	6	6	theme	extraction	891:900	arg1	those					922:926	those	922:926	those	922:926	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	4	7	theme	high-speed	713:722	arg1	centrifuges					724:734	centralized high-speed centrifuges	701:734	centralized high-speed centrifuges	701:734	However, such extraction techniques traditionally utilize spin-column techniques that in turn require centralized high-speed centrifuges.					
36126446	6	8	theme	nucleic	878:884	arg1	acid					886:889	instrument-free nucleic acid	862:889	instrument-free nucleic acid extraction methods	862:908	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	3	9	theme	DNA	569:571	arg1	extraction					534:543	the successful extraction	519:543	the successful extraction of pure patient genomic DNA from the clinical sample	519:596	Similarly, genetic disease screening is also reliant on the successful extraction of pure patient genomic DNA from the clinical sample.					
36126446	8	10	theme	instrument-free	1315:1329	arg1	manner					1331:1336	a completely instrument-free manner	1302:1336	a completely instrument-free manner using cotton and a sterilized toothpick	1302:1376	The extraction was carried out in a completely instrument-free manner using cotton and a sterilized toothpick and was completed in 30 min (with using chaotropic salt) or 10 min (without using chaotropic salt).					
36126446	9	11	theme	extracted	1497:1505	arg1	DNA					1507:1509	the extracted DNA	1493:1509	the extracted DNA	1493:1509	The quality of the extracted DNA was then probed using PCR followed by agarose gel analysis for preliminary validation of the study.					
36126446	2	12	from	sample	389:394	arg1	purification					330:341	prior purification	324:341	prior purification	324:341	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	2	12	from	sample	389:394	arg1	extraction					346:355	extraction	346:355	extraction	346:355	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	11	13	theme	extracted	1842:1850	arg1	DNA					1852:1854	extracted DNA	1842:1854	extracted DNA	1842:1854	Besides, percent similarity score obtained from the Sanger sequencing experiments probed the feasibility of extracted DNA towards polymerase amplification with fluorescent nucleotide incorporation.					
36126446	2	14	theme	prior	324:328	arg1	purification					330:341	prior purification	324:341	prior purification	324:341	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	11	15	theme	fluorescent	1894:1904	arg1	incorporation					1917:1929	fluorescent nucleotide incorporation	1894:1929	fluorescent nucleotide incorporation	1894:1929	Besides, percent similarity score obtained from the Sanger sequencing experiments probed the feasibility of extracted DNA towards polymerase amplification with fluorescent nucleotide incorporation.					
36126446	7	16	theme	extract	1188:1194	arg1	gDNA					1209:1212	gDNA	1209:1212	gDNA	1209:1212	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	7	16	theme	extract	1188:1194	arg1	DNA					1204:1206	extract genomic DNA	1188:1206	extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate	1188:1265	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	8	17	theme	sterilized	1357:1366	arg1	toothpick					1368:1376	a sterilized toothpick	1355:1376	a sterilized toothpick	1355:1376	The extraction was carried out in a completely instrument-free manner using cotton and a sterilized toothpick and was completed in 30 min (with using chaotropic salt) or 10 min (without using chaotropic salt).					
36126446	7	18	theme	biomaterial	1173:1183	arg1	over-the-counter					1156:1171	a readily available over-the-counter	1136:1171	a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate	1136:1265	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	7	18	theme	biomaterial	1173:1183	arg1	cotton					1128:1133	medical-grade cotton	1114:1133	medical-grade cotton	1114:1133	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	7	19	theme	medical-grade	1114:1126	arg1	over-the-counter					1156:1171	a readily available over-the-counter	1136:1171	a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate	1136:1265	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	7	19	theme	medical-grade	1114:1126	arg1	cotton					1128:1133	medical-grade cotton	1114:1133	medical-grade cotton	1114:1133	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	1	20	theme	NAAT	148:151	arg1	intervention					188:199	the primary intervention	176:199	the primary intervention for pathogen molecular diagnostics	176:234	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	1	20	theme	NAAT	148:151	arg1	detection					163:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	6	21	theme	diagnosis	1032:1040	arg1	workflows					1042:1050	NAAT-mediated molecular diagnosis workflows	1008:1050	NAAT-mediated molecular diagnosis workflows in resource-constrained settings	1008:1083	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	3	22	from	sample	591:596	arg1	DNA					569:571	pure patient genomic DNA	548:571	pure patient genomic DNA from the clinical sample	548:596	Similarly, genetic disease screening is also reliant on the successful extraction of pure patient genomic DNA from the clinical sample.					
36126446	3	22	from	sample	591:596	arg1	extraction					534:543	the successful extraction	519:543	the successful extraction of pure patient genomic DNA from the clinical sample	519:596	Similarly, genetic disease screening is also reliant on the successful extraction of pure patient genomic DNA from the clinical sample.					
36126446	11	23	theme	sequencing	1793:1802	arg1	experiments					1804:1814	the Sanger sequencing experiments	1782:1814	the Sanger sequencing experiments	1782:1814	Besides, percent similarity score obtained from the Sanger sequencing experiments probed the feasibility of extracted DNA towards polymerase amplification with fluorescent nucleotide incorporation.					
36126446	7	24	theme	available	1146:1154	arg1	over-the-counter					1156:1171	a readily available over-the-counter	1136:1171	a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate	1136:1265	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	7	24	theme	available	1146:1154	arg1	cotton					1128:1133	medical-grade cotton	1114:1133	medical-grade cotton	1114:1133	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	2	25	theme	chain	294:298	arg1	qPCR					310:313	qPCR	310:313	qPCR	310:313	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	2	25	theme	chain	294:298	arg1	reaction					300:307	quantitative real-time polymerase chain reaction	260:307	quantitative real-time polymerase chain reaction (qPCR)	260:314	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	1	26	theme	-assisted	153:161	arg1	intervention					188:199	the primary intervention	176:199	the primary intervention for pathogen molecular diagnostics	176:234	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	1	26	theme	-assisted	153:161	arg1	detection					163:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	0	27	theme	over-the-counter	71:86	arg1	cotton					102:107	toothpick-actuated over-the-counter medical-grade cotton	52:107	toothpick-actuated over-the-counter medical-grade cotton	52:107	Nucleic acid extraction from complex biofluid using toothpick-actuated over-the-counter medical-grade cotton.					
36126446	2	28	theme	real-time	273:281	arg1	qPCR					310:313	qPCR	310:313	qPCR	310:313	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	2	28	theme	real-time	273:281	arg1	reaction					300:307	quantitative real-time polymerase chain reaction	260:307	quantitative real-time polymerase chain reaction (qPCR)	260:314	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	9	29	theme	gel	1557:1559	arg1	analysis					1561:1568	agarose gel analysis	1549:1568	agarose gel analysis	1549:1568	The quality of the extracted DNA was then probed using PCR followed by agarose gel analysis for preliminary validation of the study.					
36126446	5	30	theme	potential	754:762	arg1	deployment					764:773	any potential deployment	750:773	any potential deployment of qPCR- or PCR-like NAAT methods in resource-constrained settings	750:840	This hinders any potential deployment of qPCR- or PCR-like NAAT methods in resource-constrained settings.					
36126446	12	31	theme	DNA	2142:2144	arg1	quality					2117:2123	the quality	2113:2123	the quality of the extracted DNA	2113:2144	Overall, our method demonstrated that DNA extraction could be performed utilizing toothpick-mounted cotton both with or without using a chaotropic salt, albeit with a difference in the quality of the extracted DNA.					
36126446	11	32	with	amplification	1875:1887	arg1	incorporation					1917:1929	fluorescent nucleotide incorporation	1894:1929	fluorescent nucleotide incorporation	1894:1929	Besides, percent similarity score obtained from the Sanger sequencing experiments probed the feasibility of extracted DNA towards polymerase amplification with fluorescent nucleotide incorporation.					
36126446	1	33	theme	primary	180:186	arg1	intervention					188:199	the primary intervention	176:199	the primary intervention for pathogen molecular diagnostics	176:234	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	1	33	theme	primary	180:186	arg1	detection					163:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	5	34	theme	NAAT	796:799	arg1	methods					801:807	qPCR- or PCR-like NAAT methods	778:807	qPCR- or PCR-like NAAT methods	778:807	This hinders any potential deployment of qPCR- or PCR-like NAAT methods in resource-constrained settings.					
36126446	12	35	theme	toothpick-mounted	2014:2030	arg1	cotton					2032:2037	toothpick-mounted cotton	2014:2037	toothpick-mounted cotton both with or without using a chaotropic salt	2014:2082	Overall, our method demonstrated that DNA extraction could be performed utilizing toothpick-mounted cotton both with or without using a chaotropic salt, albeit with a difference in the quality of the extracted DNA.					
36126446	8	36	theme	chaotropic	1418:1427	arg1	salt					1429:1432	chaotropic salt	1418:1432	chaotropic salt	1418:1432	The extraction was carried out in a completely instrument-free manner using cotton and a sterilized toothpick and was completed in 30 min (with using chaotropic salt) or 10 min (without using chaotropic salt).					
36126446	12	37	theme	DNA	1970:1972	arg1	extraction					1974:1983	DNA extraction	1970:1983	DNA extraction	1970:1983	Overall, our method demonstrated that DNA extraction could be performed utilizing toothpick-mounted cotton both with or without using a chaotropic salt, albeit with a difference in the quality of the extracted DNA.					
36126446	0	38	theme	medical-grade	88:100	arg1	cotton					102:107	toothpick-actuated over-the-counter medical-grade cotton	52:107	toothpick-actuated over-the-counter medical-grade cotton	52:107	Nucleic acid extraction from complex biofluid using toothpick-actuated over-the-counter medical-grade cotton.					
36126446	0	39	theme	Nucleic	0:6	arg1	extraction					13:22	Nucleic acid extraction	0:22	Nucleic acid extraction from complex biofluid using toothpick-actuated over-the-counter medical-grade cotton.	0:108	Nucleic acid extraction from complex biofluid using toothpick-actuated over-the-counter medical-grade cotton.					
36126446	1	40	theme	acid	118:121	arg1	intervention					188:199	the primary intervention	176:199	the primary intervention for pathogen molecular diagnostics	176:234	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	1	40	theme	acid	118:121	arg1	detection					163:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	11	41	theme	percent	1743:1749	arg1	score					1762:1766	percent similarity score	1743:1766	percent similarity score obtained from the Sanger sequencing experiments	1743:1814	Besides, percent similarity score obtained from the Sanger sequencing experiments probed the feasibility of extracted DNA towards polymerase amplification with fluorescent nucleotide incorporation.					
36126446	7	42	theme	%	1244:1244	arg1	serum					1246:1250	60 % serum or cell lysate	1241:1265	serum	1246:1250	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	1	43	theme	technique	137:145	arg1	intervention					188:199	the primary intervention	176:199	the primary intervention for pathogen molecular diagnostics	176:234	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	1	43	theme	technique	137:145	arg1	detection					163:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	3	44	theme	disease	482:488	arg1	screening					490:498	genetic disease screening	474:498	genetic disease screening	474:498	Similarly, genetic disease screening is also reliant on the successful extraction of pure patient genomic DNA from the clinical sample.					
36126446	10	45	theme	serum	1714:1718	arg1	composition					1720:1730	serum composition	1714:1730	serum composition	1714:1730	The qPCR experiments then quantitatively established the extraction efficiency (0.3-27 %, depending on serum composition).					
36126446	0	46	theme	complex	29:35	arg1	biofluid					37:44	complex biofluid	29:44	complex biofluid using toothpick-actuated over-the-counter medical-grade cotton	29:107	Nucleic acid extraction from complex biofluid using toothpick-actuated over-the-counter medical-grade cotton.					
36126446	6	47	from	workflows	1042:1050	arg1	settings					1076:1083	resource-constrained settings	1055:1083	resource-constrained settings	1055:1083	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	8	48	theme	chaotropic	1460:1469	arg1	salt					1471:1474	chaotropic salt	1460:1474	chaotropic salt	1460:1474	The extraction was carried out in a completely instrument-free manner using cotton and a sterilized toothpick and was completed in 30 min (with using chaotropic salt) or 10 min (without using chaotropic salt).					
36126446	2	49	theme	acid	375:378	arg1	purification					330:341	prior purification	324:341	prior purification	324:341	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	2	49	theme	acid	375:378	arg1	extraction					346:355	extraction	346:355	extraction	346:355	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	2	50	theme	target	360:365	arg1	acid					375:378	target nucleic acid	360:378	target nucleic acid	360:378	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	10	51	dep	depending	1701:1709	arg1	%					1698:1698	0.3-27 %	1691:1698	0.3-27 %	1691:1698	The qPCR experiments then quantitatively established the extraction efficiency (0.3-27 %, depending on serum composition).					
36126446	3	52	theme	pure	548:551	arg1	DNA					569:571	pure patient genomic DNA	548:571	pure patient genomic DNA from the clinical sample	548:596	Similarly, genetic disease screening is also reliant on the successful extraction of pure patient genomic DNA from the clinical sample.					
36126446	6	53	theme	molecular	1022:1030	arg1	workflows					1042:1050	NAAT-mediated molecular diagnosis workflows	1008:1050	NAAT-mediated molecular diagnosis workflows in resource-constrained settings	1008:1083	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	4	54	theme	spin-column	657:667	arg1	techniques					669:678	spin-column techniques	657:678	spin-column techniques that in turn require centralized high-speed centrifuges	657:734	However, such extraction techniques traditionally utilize spin-column techniques that in turn require centralized high-speed centrifuges.					
36126446	10	55	theme	qPCR	1615:1618	arg1	experiments					1620:1630	The qPCR experiments	1611:1630	The qPCR experiments	1611:1630	The qPCR experiments then quantitatively established the extraction efficiency (0.3-27 %, depending on serum composition).					
36126446	6	56	theme	methods	902:908	arg1	development					847:857	The development	843:857	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials	843:964	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	5	57	from	deployment	764:773	arg1	settings					833:840	resource-constrained settings	812:840	resource-constrained settings	812:840	This hinders any potential deployment of qPCR- or PCR-like NAAT methods in resource-constrained settings.					
36126446	4	58	theme	extraction	613:622	arg1	techniques					624:633	such extraction techniques	608:633	such extraction techniques	608:633	However, such extraction techniques traditionally utilize spin-column techniques that in turn require centralized high-speed centrifuges.					
36126446	6	59	theme	acid	886:889	arg1	methods					902:908	instrument-free nucleic acid extraction methods	862:908	instrument-free nucleic acid extraction methods	862:908	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	6	59	theme	acid	886:889	arg1	those					922:926	those	922:926	those	922:926	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	3	60	theme	genomic	561:567	arg1	DNA					569:571	pure patient genomic DNA	548:571	pure patient genomic DNA from the clinical sample	548:596	Similarly, genetic disease screening is also reliant on the successful extraction of pure patient genomic DNA from the clinical sample.					
36126446	6	61	theme	great	978:982	arg1	interest					984:991	great interest	978:991	great interest	978:991	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	4	62	theme	centralized	701:711	arg1	centrifuges					724:734	centralized high-speed centrifuges	701:734	centralized high-speed centrifuges	701:734	However, such extraction techniques traditionally utilize spin-column techniques that in turn require centralized high-speed centrifuges.					
36126446	6	63	theme	instrument-free	862:876	arg1	acid					886:889	instrument-free nucleic acid	862:889	instrument-free nucleic acid extraction methods	862:908	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	1	64	theme	pathogen	205:212	arg1	diagnostics					224:234	pathogen molecular diagnostics	205:234	pathogen molecular diagnostics	205:234	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	11	65	theme	polymerase	1864:1873	arg1	amplification					1875:1887	polymerase amplification	1864:1887	polymerase amplification with fluorescent nucleotide incorporation	1864:1929	Besides, percent similarity score obtained from the Sanger sequencing experiments probed the feasibility of extracted DNA towards polymerase amplification with fluorescent nucleotide incorporation.					
36126446	7	66	theme	genomic	1196:1202	arg1	gDNA					1209:1212	gDNA	1209:1212	gDNA	1209:1212	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	7	66	theme	genomic	1196:1202	arg1	DNA					1204:1206	extract genomic DNA	1188:1206	extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate	1188:1265	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	3	67	theme	clinical	582:589	arg1	sample					591:596	the clinical sample	578:596	the clinical sample	578:596	Similarly, genetic disease screening is also reliant on the successful extraction of pure patient genomic DNA from the clinical sample.					
36126446	9	68	theme	DNA	1507:1509	arg1	quality					1482:1488	The quality	1478:1488	The quality of the extracted DNA	1478:1509	The quality of the extracted DNA was then probed using PCR followed by agarose gel analysis for preliminary validation of the study.					
36126446	12	69	theme	chaotropic	2068:2077	arg1	salt					2079:2082	a chaotropic salt	2066:2082	a chaotropic salt	2066:2082	Overall, our method demonstrated that DNA extraction could be performed utilizing toothpick-mounted cotton both with or without using a chaotropic salt, albeit with a difference in the quality of the extracted DNA.					
36126446	12	70	dep	cotton	2032:2037	arg1	with					2044:2047	with	2044:2047	with	2044:2047	Overall, our method demonstrated that DNA extraction could be performed utilizing toothpick-mounted cotton both with or without using a chaotropic salt, albeit with a difference in the quality of the extracted DNA.					
36126446	12	70	dep	cotton	2032:2037	arg1	using					2060:2064	using	2060:2064	using a chaotropic salt	2060:2082	Overall, our method demonstrated that DNA extraction could be performed utilizing toothpick-mounted cotton both with or without using a chaotropic salt, albeit with a difference in the quality of the extracted DNA.					
36126446	3	71	theme	successful	523:532	arg1	extraction					534:543	the successful extraction	519:543	the successful extraction of pure patient genomic DNA from the clinical sample	519:596	Similarly, genetic disease screening is also reliant on the successful extraction of pure patient genomic DNA from the clinical sample.					
36126446	5	72	theme	qPCR-	778:782	arg1	methods					801:807	qPCR- or PCR-like NAAT methods	778:807	qPCR- or PCR-like NAAT methods	778:807	This hinders any potential deployment of qPCR- or PCR-like NAAT methods in resource-constrained settings.					
36126446	2	73	theme	quantitative	260:271	arg1	qPCR					310:313	qPCR	310:313	qPCR	310:313	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	2	73	theme	quantitative	260:271	arg1	reaction					300:307	quantitative real-time polymerase chain reaction	260:307	quantitative real-time polymerase chain reaction (qPCR)	260:314	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	1	74	theme	molecular	214:222	arg1	diagnostics					224:234	pathogen molecular diagnostics	205:234	pathogen molecular diagnostics	205:234	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	6	75	theme	resource-constrained	1055:1074	arg1	settings					1076:1083	resource-constrained settings	1055:1083	resource-constrained settings	1055:1083	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	10	76	theme	extraction	1668:1677	arg1	efficiency					1679:1688	the extraction efficiency	1664:1688	the extraction efficiency (0.3-27 %, depending on serum composition)	1664:1731	The qPCR experiments then quantitatively established the extraction efficiency (0.3-27 %, depending on serum composition).					
36126446	11	77	theme	nucleotide	1906:1915	arg1	incorporation					1917:1929	fluorescent nucleotide incorporation	1894:1929	fluorescent nucleotide incorporation	1894:1929	Besides, percent similarity score obtained from the Sanger sequencing experiments probed the feasibility of extracted DNA towards polymerase amplification with fluorescent nucleotide incorporation.					
36126446	2	78	contain	contains	431:438	arg2	inhibitors					451:460	polymerase inhibitors	440:460	polymerase inhibitors	440:460	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	2	78	contain	contains	431:438	arg1	latter					418:423	latter	418:423	latter	418:423	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	5	79	theme	resource-constrained	812:831	arg1	settings					833:840	resource-constrained settings	812:840	resource-constrained settings	812:840	This hinders any potential deployment of qPCR- or PCR-like NAAT methods in resource-constrained settings.					
36126446	2	80	theme	polymerase	283:292	arg1	qPCR					310:313	qPCR	310:313	qPCR	310:313	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	2	80	theme	polymerase	283:292	arg1	reaction					300:307	quantitative real-time polymerase chain reaction	260:307	quantitative real-time polymerase chain reaction (qPCR)	260:314	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	9	81	theme	agarose	1549:1555	arg1	analysis					1561:1568	agarose gel analysis	1549:1568	agarose gel analysis	1549:1568	The quality of the extracted DNA was then probed using PCR followed by agarose gel analysis for preliminary validation of the study.					
36126446	11	82	theme	Sanger	1786:1791	arg1	sequencing					1793:1802	Sanger sequencing	1786:1802	the Sanger sequencing experiments	1782:1814	Besides, percent similarity score obtained from the Sanger sequencing experiments probed the feasibility of extracted DNA towards polymerase amplification with fluorescent nucleotide incorporation.					
36126446	12	83	theme	extracted	2132:2140	arg1	DNA					2142:2144	the extracted DNA	2128:2144	the extracted DNA	2128:2144	Overall, our method demonstrated that DNA extraction could be performed utilizing toothpick-mounted cotton both with or without using a chaotropic salt, albeit with a difference in the quality of the extracted DNA.					
36126446	5	84	theme	PCR-like	787:794	arg1	methods					801:807	qPCR- or PCR-like NAAT methods	778:807	qPCR- or PCR-like NAAT methods	778:807	This hinders any potential deployment of qPCR- or PCR-like NAAT methods in resource-constrained settings.					
36126446	9	85	theme	preliminary	1574:1584	arg1	validation					1586:1595	preliminary validation	1574:1595	preliminary validation of the study	1574:1608	The quality of the extracted DNA was then probed using PCR followed by agarose gel analysis for preliminary validation of the study.					
36126446	7	86	theme	cell	1255:1258	arg1	lysate					1260:1265	60 % serum or cell lysate	1241:1265	lysate	1260:1265	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	0	87	from	biofluid	37:44	arg1	extraction					13:22	Nucleic acid extraction	0:22	Nucleic acid extraction from complex biofluid using toothpick-actuated over-the-counter medical-grade cotton.	0:108	Nucleic acid extraction from complex biofluid using toothpick-actuated over-the-counter medical-grade cotton.					
36126446	5	88	theme	methods	801:807	arg1	deployment					764:773	any potential deployment	750:773	any potential deployment of qPCR- or PCR-like NAAT methods in resource-constrained settings	750:840	This hinders any potential deployment of qPCR- or PCR-like NAAT methods in resource-constrained settings.					
36126446	0	89	theme	acid	8:11	arg1	extraction					13:22	Nucleic acid extraction	0:22	Nucleic acid extraction from complex biofluid using toothpick-actuated over-the-counter medical-grade cotton.	0:108	Nucleic acid extraction from complex biofluid using toothpick-actuated over-the-counter medical-grade cotton.					
36126446	11	90	theme	DNA	1852:1854	arg1	feasibility					1827:1837	the feasibility	1823:1837	the feasibility of extracted DNA	1823:1854	Besides, percent similarity score obtained from the Sanger sequencing experiments probed the feasibility of extracted DNA towards polymerase amplification with fluorescent nucleotide incorporation.					
36126446	1	91	theme	Nucleic	110:116	arg1	intervention					188:199	the primary intervention	176:199	the primary intervention for pathogen molecular diagnostics	176:234	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	1	91	theme	Nucleic	110:116	arg1	detection					163:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	2	92	theme	interest	399:406	arg1	sample					389:394	the sample	385:394	the sample of interest	385:406	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	7	93	theme	60 	1241:1243	arg1	serum					1246:1250	60 % serum or cell lysate	1241:1265	serum	1246:1250	In this report, we screened medical-grade cotton, a readily available over-the-counter biomaterial to extract genomic DNA (gDNA) spiked in 30 %, 45 %, and 60 % serum or cell lysate.					
36126446	1	94	theme	amplification	123:135	arg1	intervention					188:199	the primary intervention	176:199	the primary intervention for pathogen molecular diagnostics	176:234	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	1	94	theme	amplification	123:135	arg1	detection					163:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection	110:171	Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics.					
36126446	2	95	theme	polymerase	440:449	arg1	inhibitors					451:460	polymerase inhibitors	440:460	polymerase inhibitors	440:460	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	0	96	theme	toothpick-actuated	52:69	arg1	cotton					102:107	toothpick-actuated over-the-counter medical-grade cotton	52:107	toothpick-actuated over-the-counter medical-grade cotton	52:107	Nucleic acid extraction from complex biofluid using toothpick-actuated over-the-counter medical-grade cotton.					
36126446	6	97	theme	available	946:954	arg1	materials					956:964	readily available materials	938:964	readily available materials	938:964	The development of instrument-free nucleic acid extraction methods, especially those utilizing readily available materials would be of great interest and benefit to NAAT-mediated molecular diagnosis workflows in resource-constrained settings.					
36126446	2	98	theme	nucleic	367:373	arg1	acid					375:378	target nucleic acid	360:378	target nucleic acid	360:378	However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of target nucleic acid from the sample of interest since the latter often contains polymerase inhibitors.					
36126446	11	99	theme	similarity	1751:1760	arg1	score					1762:1766	percent similarity score	1743:1766	percent similarity score obtained from the Sanger sequencing experiments	1743:1814	Besides, percent similarity score obtained from the Sanger sequencing experiments probed the feasibility of extracted DNA towards polymerase amplification with fluorescent nucleotide incorporation.					
36126446	3	100	theme	genetic	474:480	arg1	screening					490:498	genetic disease screening	474:498	genetic disease screening	474:498	Similarly, genetic disease screening is also reliant on the successful extraction of pure patient genomic DNA from the clinical sample.					
36126446	3	101	theme	patient	553:559	arg1	DNA					569:571	pure patient genomic DNA	548:571	pure patient genomic DNA from the clinical sample	548:596	Similarly, genetic disease screening is also reliant on the successful extraction of pure patient genomic DNA from the clinical sample.					
35452117	6	0	theme	A.	911:912	arg1	PG					926:927	the A. muciniphila PG	907:927	the A. muciniphila PG	907:927	The immunological assays revealed that muropeptides released from the A. muciniphila PG could both activate the intracellular NOD1 and NOD2 receptors to a comparable extent as muropeptides from Escherichia coli BW25113.					
35452117	8	1	theme	gut	1256:1258	arg1	members					1271:1277	key gut microbiota members	1252:1277	key gut microbiota members	1252:1277	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	8	2	theme	steering	1297:1304	arg1	interactions					1322:1333	steering host-microbiome interactions	1297:1333	steering host-microbiome interactions	1297:1333	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	8	3	theme	cell	1224:1227	arg1	structures					1238:1247	cell envelope structures	1224:1247	cell envelope structures of key gut microbiota members	1224:1277	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	2	4	theme	cell	238:241	arg1	components					252:261	Various cell envelope components	230:261	Various cell envelope components	230:261	Various cell envelope components have been identified to play a critical role in the immune signaling of A. muciniphila, but the chemical composition and role of peptidoglycan (PG) remained elusive.					
35452117	6	5	dep	Escherichia	1035:1045	arg1	coli					1047:1050	coli	1047:1050	coli	1047:1050	The immunological assays revealed that muropeptides released from the A. muciniphila PG could both activate the intracellular NOD1 and NOD2 receptors to a comparable extent as muropeptides from Escherichia coli BW25113.					
35452117	0	6	theme	muropeptides	104:115	arg1	properties					90:99	immunostimulatory properties	72:99	immunostimulatory properties	72:99	Peptidoglycan from Akkermansia muciniphila MucT: chemical structure and immunostimulatory properties of muropeptides.					
35452117	0	6	theme	muropeptides	104:115	arg1	structure					58:66	chemical structure	49:66	chemical structure	49:66	Peptidoglycan from Akkermansia muciniphila MucT: chemical structure and immunostimulatory properties of muropeptides.					
35452117	7	7	theme	PG	1124:1125	arg1	mechanism					1158:1166	a NOD-1 evasion mechanism	1142:1166	a NOD-1 evasion mechanism	1142:1166	These data challenge the hypothesis that non-N-acetylattion of PG can be used as a NOD-1 evasion mechanism.					
35452117	7	7	theme	PG	1124:1125	arg1	non-N-acetylattion					1102:1119	non-N-acetylattion	1102:1119	non-N-acetylattion of PG	1102:1125	These data challenge the hypothesis that non-N-acetylattion of PG can be used as a NOD-1 evasion mechanism.					
35452117	2	8	theme	critical	294:301	arg1	role					303:306	a critical role	292:306	a critical role	292:306	Various cell envelope components have been identified to play a critical role in the immune signaling of A. muciniphila, but the chemical composition and role of peptidoglycan (PG) remained elusive.					
35452117	6	9	theme	NOD2	976:979	arg1	receptors					981:989	the intracellular NOD1 and NOD2 receptors	949:989	receptors	981:989	The immunological assays revealed that muropeptides released from the A. muciniphila PG could both activate the intracellular NOD1 and NOD2 receptors to a comparable extent as muropeptides from Escherichia coli BW25113.					
35452117	8	10	from	diversity	1211:1219	arg1	interactions					1322:1333	steering host-microbiome interactions	1297:1333	steering host-microbiome interactions	1297:1333	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	2	11	dep	composition	368:378	arg1	the					355:357	the	355:357	the	355:357	Various cell envelope components have been identified to play a critical role in the immune signaling of A. muciniphila, but the chemical composition and role of peptidoglycan (PG) remained elusive.					
35452117	8	12	theme	new	1189:1191	arg1	insights					1193:1200	new insights	1189:1200	new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions	1189:1333	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	1	13	theme	gut	189:191	arg1	function					201:208	the gut barrier function	185:208	the gut barrier function in mice and humans	185:227	Akkermansia muciniphila is an intestinal symbiont known to improve the gut barrier function in mice and humans.					
35452117	6	14	dep	A.	911:912	arg1	muciniphila					914:924	muciniphila	914:924	muciniphila	914:924	The immunological assays revealed that muropeptides released from the A. muciniphila PG could both activate the intracellular NOD1 and NOD2 receptors to a comparable extent as muropeptides from Escherichia coli BW25113.					
35452117	1	15	theme	barrier	193:199	arg1	function					201:208	the gut barrier function	185:208	the gut barrier function in mice and humans	185:227	Akkermansia muciniphila is an intestinal symbiont known to improve the gut barrier function in mice and humans.					
35452117	5	16	theme	N-acetylmuramic	783:797	arg1	acid					799:802	N-acetylmuramic acid	783:802	the N-acetylmuramic acid (MurNAc) subunits	779:820	Some of the N-acetylmuramic acid (MurNAc) subunits were O-acetylated.					
35452117	5	16	theme	N-acetylmuramic	783:797	arg1	MurNAc					805:810	MurNAc	805:810	MurNAc	805:810	Some of the N-acetylmuramic acid (MurNAc) subunits were O-acetylated.					
35452117	0	17	from	MucT	43:46	arg1	Peptidoglycan					0:12	Peptidoglycan	0:12	Peptidoglycan from Akkermansia muciniphila MucT: chemical structure and immunostimulatory properties of muropeptides.	0:116	Peptidoglycan from Akkermansia muciniphila MucT: chemical structure and immunostimulatory properties of muropeptides.					
35452117	5	18	theme	acid	799:802	arg1	subunits					813:820	the N-acetylmuramic acid (MurNAc) subunits	779:820	the N-acetylmuramic acid (MurNAc) subunits	779:820	Some of the N-acetylmuramic acid (MurNAc) subunits were O-acetylated.					
35452117	8	19	theme	envelope	1229:1236	arg1	structures					1238:1247	cell envelope structures	1224:1247	cell envelope structures of key gut microbiota members	1224:1277	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	6	20	from	BW25113	1052:1058	arg1	muropeptides					1017:1028	muropeptides	1017:1028	muropeptides from Escherichia coli BW25113	1017:1058	The immunological assays revealed that muropeptides released from the A. muciniphila PG could both activate the intracellular NOD1 and NOD2 receptors to a comparable extent as muropeptides from Escherichia coli BW25113.					
35452117	3	21	theme	immune	548:553	arg1	capacity					565:572	their immune signaling capacity	542:572	their immune signaling capacity	542:572	Here, we isolated PG fragments from A. muciniphila MucT (ATCC BAA-835), analyzed their composition and evaluated their immune signaling capacity.					
35452117	8	22	theme	structures	1238:1247	arg1	role					1289:1292	their role	1283:1292	their role in steering host-microbiome interactions	1283:1333	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	8	22	theme	structures	1238:1247	arg1	diversity					1211:1219	the diversity	1207:1219	the diversity of cell envelope structures of key gut microbiota members	1207:1277	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	7	23	theme	evasion	1150:1156	arg1	mechanism					1158:1166	a NOD-1 evasion mechanism	1142:1166	a NOD-1 evasion mechanism	1142:1166	These data challenge the hypothesis that non-N-acetylattion of PG can be used as a NOD-1 evasion mechanism.					
35452117	7	23	theme	evasion	1150:1156	arg1	non-N-acetylattion					1102:1119	non-N-acetylattion	1102:1119	non-N-acetylattion of PG	1102:1125	These data challenge the hypothesis that non-N-acetylattion of PG can be used as a NOD-1 evasion mechanism.					
35452117	1	24	theme	Akkermansia	118:128	arg1	symbiont					159:166	an intestinal symbiont	145:166	an intestinal symbiont known to improve the gut barrier function in mice and humans	145:227	Akkermansia muciniphila is an intestinal symbiont known to improve the gut barrier function in mice and humans.					
35452117	1	24	theme	Akkermansia	118:128	arg1	muciniphila					130:140	Akkermansia muciniphila	118:140	Akkermansia muciniphila	118:140	Akkermansia muciniphila is an intestinal symbiont known to improve the gut barrier function in mice and humans.					
35452117	3	25	theme	PG	447:448	arg1	fragments					450:458	PG fragments	447:458	PG fragments	447:458	Here, we isolated PG fragments from A. muciniphila MucT (ATCC BAA-835), analyzed their composition and evaluated their immune signaling capacity.					
35452117	2	26	theme	immune	315:320	arg1	signaling					322:330	the immune signaling	311:330	the immune signaling of A. muciniphila	311:348	Various cell envelope components have been identified to play a critical role in the immune signaling of A. muciniphila, but the chemical composition and role of peptidoglycan (PG) remained elusive.					
35452117	6	27	theme	intracellular	953:965	arg1	NOD1					967:970	the intracellular NOD1 and NOD2 receptors	949:989	NOD1	967:970	The immunological assays revealed that muropeptides released from the A. muciniphila PG could both activate the intracellular NOD1 and NOD2 receptors to a comparable extent as muropeptides from Escherichia coli BW25113.					
35452117	4	28	theme	N-acetylglucosamine	750:768	arg1	deacetylation					733:745	deacetylation	733:745	deacetylation of N-acetylglucosamine	733:768	Structurally, the PG of A. muciniphila was found to be noteworthy due of the presence of some nonacetylated glucosamine residues, which presumably stems from deacetylation of N-acetylglucosamine.					
35452117	8	29	theme	key	1252:1254	arg1	members					1271:1277	key gut microbiota members	1252:1277	key gut microbiota members	1252:1277	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	6	30	theme	immunological	845:857	arg1	assays					859:864	The immunological assays	841:864	The immunological assays	841:864	The immunological assays revealed that muropeptides released from the A. muciniphila PG could both activate the intracellular NOD1 and NOD2 receptors to a comparable extent as muropeptides from Escherichia coli BW25113.					
35452117	6	31	theme	Escherichia	1035:1045	arg1	BW25113					1052:1058	Escherichia coli BW25113	1035:1058	Escherichia coli BW25113	1035:1058	The immunological assays revealed that muropeptides released from the A. muciniphila PG could both activate the intracellular NOD1 and NOD2 receptors to a comparable extent as muropeptides from Escherichia coli BW25113.					
35452117	2	32	theme	peptidoglycan	392:404	arg1	role					384:387	role	384:387	role	384:387	Various cell envelope components have been identified to play a critical role in the immune signaling of A. muciniphila, but the chemical composition and role of peptidoglycan (PG) remained elusive.					
35452117	2	32	theme	peptidoglycan	392:404	arg1	composition					368:378	chemical composition	359:378	chemical composition	359:378	Various cell envelope components have been identified to play a critical role in the immune signaling of A. muciniphila, but the chemical composition and role of peptidoglycan (PG) remained elusive.					
35452117	7	33	theme	NOD-1	1144:1148	arg1	mechanism					1158:1166	a NOD-1 evasion mechanism	1142:1166	a NOD-1 evasion mechanism	1142:1166	These data challenge the hypothesis that non-N-acetylattion of PG can be used as a NOD-1 evasion mechanism.					
35452117	7	33	theme	NOD-1	1144:1148	arg1	non-N-acetylattion					1102:1119	non-N-acetylattion	1102:1119	non-N-acetylattion of PG	1102:1125	These data challenge the hypothesis that non-N-acetylattion of PG can be used as a NOD-1 evasion mechanism.					
35452117	8	34	theme	members	1271:1277	arg1	structures					1238:1247	cell envelope structures	1224:1247	cell envelope structures of key gut microbiota members	1224:1277	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	2	35	theme	envelope	243:250	arg1	components					252:261	Various cell envelope components	230:261	Various cell envelope components	230:261	Various cell envelope components have been identified to play a critical role in the immune signaling of A. muciniphila, but the chemical composition and role of peptidoglycan (PG) remained elusive.					
35452117	8	36	theme	microbiota	1260:1269	arg1	members					1271:1277	key gut microbiota members	1252:1277	key gut microbiota members	1252:1277	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	3	37	theme	signaling	555:563	arg1	capacity					565:572	their immune signaling capacity	542:572	their immune signaling capacity	542:572	Here, we isolated PG fragments from A. muciniphila MucT (ATCC BAA-835), analyzed their composition and evaluated their immune signaling capacity.					
35452117	4	38	theme	nonacetylated	669:681	arg1	residues					695:702	some nonacetylated glucosamine residues	664:702	some nonacetylated glucosamine residues	664:702	Structurally, the PG of A. muciniphila was found to be noteworthy due of the presence of some nonacetylated glucosamine residues, which presumably stems from deacetylation of N-acetylglucosamine.					
35452117	0	39	theme	chemical	49:56	arg1	structure					58:66	chemical structure	49:66	chemical structure	49:66	Peptidoglycan from Akkermansia muciniphila MucT: chemical structure and immunostimulatory properties of muropeptides.					
35452117	7	40	used	used	1134:1137	arg2	mechanism					1158:1166	a NOD-1 evasion mechanism	1142:1166	a NOD-1 evasion mechanism	1142:1166	These data challenge the hypothesis that non-N-acetylattion of PG can be used as a NOD-1 evasion mechanism.					
35452117	7	40	used	used	1134:1137	arg2	non-N-acetylattion					1102:1119	non-N-acetylattion	1102:1119	non-N-acetylattion of PG	1102:1125	These data challenge the hypothesis that non-N-acetylattion of PG can be used as a NOD-1 evasion mechanism.					
35452117	2	41	theme	Various	230:236	arg1	components					252:261	Various cell envelope components	230:261	Various cell envelope components	230:261	Various cell envelope components have been identified to play a critical role in the immune signaling of A. muciniphila, but the chemical composition and role of peptidoglycan (PG) remained elusive.					
35452117	2	42	theme	muciniphila	338:348	arg1	signaling					322:330	the immune signaling	311:330	the immune signaling of A. muciniphila	311:348	Various cell envelope components have been identified to play a critical role in the immune signaling of A. muciniphila, but the chemical composition and role of peptidoglycan (PG) remained elusive.					
35452117	8	43	from	role	1289:1292	arg1	interactions					1322:1333	steering host-microbiome interactions	1297:1333	steering host-microbiome interactions	1297:1333	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	1	44	theme	intestinal	148:157	arg1	symbiont					159:166	an intestinal symbiont	145:166	an intestinal symbiont known to improve the gut barrier function in mice and humans	145:227	Akkermansia muciniphila is an intestinal symbiont known to improve the gut barrier function in mice and humans.					
35452117	1	44	theme	intestinal	148:157	arg1	muciniphila					130:140	Akkermansia muciniphila	118:140	Akkermansia muciniphila	118:140	Akkermansia muciniphila is an intestinal symbiont known to improve the gut barrier function in mice and humans.					
35452117	1	45	from	function	201:208	arg1	humans					222:227	humans	222:227	humans	222:227	Akkermansia muciniphila is an intestinal symbiont known to improve the gut barrier function in mice and humans.					
35452117	1	45	from	function	201:208	arg1	mice					213:216	mice	213:216	mice	213:216	Akkermansia muciniphila is an intestinal symbiont known to improve the gut barrier function in mice and humans.					
35452117	4	46	theme	glucosamine	683:693	arg1	residues					695:702	some nonacetylated glucosamine residues	664:702	some nonacetylated glucosamine residues	664:702	Structurally, the PG of A. muciniphila was found to be noteworthy due of the presence of some nonacetylated glucosamine residues, which presumably stems from deacetylation of N-acetylglucosamine.					
35452117	4	47	theme	residues	695:702	arg1	presence					652:659	the presence	648:659	the presence of some nonacetylated glucosamine residues, which presumably stems from deacetylation of N-acetylglucosamine	648:768	Structurally, the PG of A. muciniphila was found to be noteworthy due of the presence of some nonacetylated glucosamine residues, which presumably stems from deacetylation of N-acetylglucosamine.					
35452117	0	48	theme	immunostimulatory	72:88	arg1	properties					90:99	immunostimulatory properties	72:99	immunostimulatory properties	72:99	Peptidoglycan from Akkermansia muciniphila MucT: chemical structure and immunostimulatory properties of muropeptides.					
35452117	4	49	theme	muciniphila	602:612	arg1	PG					593:594	the PG	589:594	the PG of A. muciniphila	589:612	Structurally, the PG of A. muciniphila was found to be noteworthy due of the presence of some nonacetylated glucosamine residues, which presumably stems from deacetylation of N-acetylglucosamine.					
35452117	8	50	theme	host-microbiome	1306:1320	arg1	interactions					1322:1333	steering host-microbiome interactions	1297:1333	steering host-microbiome interactions	1297:1333	Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host-microbiome interactions.					
35452117	6	51	attach	released	893:900	arg1	PG					926:927	the A. muciniphila PG	907:927	the A. muciniphila PG	907:927	The immunological assays revealed that muropeptides released from the A. muciniphila PG could both activate the intracellular NOD1 and NOD2 receptors to a comparable extent as muropeptides from Escherichia coli BW25113.					
35452117	6	51	attach	released	893:900	arg2	muropeptides					880:891	muropeptides	880:891	muropeptides released from the A. muciniphila PG	880:927	The immunological assays revealed that muropeptides released from the A. muciniphila PG could both activate the intracellular NOD1 and NOD2 receptors to a comparable extent as muropeptides from Escherichia coli BW25113.					
35452117	0	52	dep	Peptidoglycan	0:12	arg1	properties					90:99	immunostimulatory properties	72:99	immunostimulatory properties	72:99	Peptidoglycan from Akkermansia muciniphila MucT: chemical structure and immunostimulatory properties of muropeptides.					
35452117	0	52	dep	Peptidoglycan	0:12	arg1	structure					58:66	chemical structure	49:66	chemical structure	49:66	Peptidoglycan from Akkermansia muciniphila MucT: chemical structure and immunostimulatory properties of muropeptides.					
35452117	2	53	theme	chemical	359:366	arg1	composition					368:378	chemical composition	359:378	chemical composition	359:378	Various cell envelope components have been identified to play a critical role in the immune signaling of A. muciniphila, but the chemical composition and role of peptidoglycan (PG) remained elusive.					
35452117	6	54	theme	comparable	996:1005	arg1	extent					1007:1012	a comparable extent	994:1012	a comparable extent as muropeptides from Escherichia coli BW25113	994:1058	The immunological assays revealed that muropeptides released from the A. muciniphila PG could both activate the intracellular NOD1 and NOD2 receptors to a comparable extent as muropeptides from Escherichia coli BW25113.					
36173901	2	0	theme	Enterococcus	352:363	arg1	surrogate					402:410	a nonpathogenic surrogate	386:410	a nonpathogenic surrogate	386:410	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	0	theme	Enterococcus	352:363	arg1	B-2354					378:383	Enterococcus faecium NRRL B-2354	352:383	Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate)	352:411	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	10	1	theme	faecium	2103:2109	arg1	ratios					2090:2095	the appropriate kill ratios	2069:2095	the appropriate kill ratios of E. faecium to Salmonella	2069:2123	In conclusion, rather than always requiring greater lethality of E. faecium than Salmonella, these findings suggest that sufficient pathogen controls for low-moisture foods can also be validated by thoroughly documenting the appropriate kill ratios of E. faecium to Salmonella.					
36173901	2	2	from	powder	583:588	arg1	resistance					507:516	relative thermal resistance	490:516	relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP)	490:689	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	8	3	theme	heat-treated	1578:1589	arg1	SMP					1591:1593	heat-treated SMP	1578:1593	heat-treated SMP	1578:1593	Addition of sugars to recovery media did not influence survivor resuscitation from heat-treated SMP and LSMP, confirming that microbial inactivation was impacted primarily by the thermal treatment, not the recovery step.					
36173901	7	4	theme	P	1398:1398	arg1	faecium					1389:1395	E. faecium	1386:1395	E. faecium (P < 0.05)	1386:1406	Estimated D90°C-values were approximately two times higher for E. faecium (P < 0.05) than for Salmonella in SMP, LP, and MPI, but statistically similar (P > 0.05) in LSMP.					
36173901	7	4	theme	P	1398:1398	arg1	<					1400:1400	P < 0.05	1398:1405	P < 0.05	1398:1405	Estimated D90°C-values were approximately two times higher for E. faecium (P < 0.05) than for Salmonella in SMP, LP, and MPI, but statistically similar (P > 0.05) in LSMP.					
36173901	7	5	theme	two	1365:1367	arg1	times					1369:1373	times	1369:1373	times	1369:1373	Estimated D90°C-values were approximately two times higher for E. faecium (P < 0.05) than for Salmonella in SMP, LP, and MPI, but statistically similar (P > 0.05) in LSMP.					
36173901	1	6	theme	nonpathogenic	169:181	arg1	surrogates					183:192	nonpathogenic surrogates	169:192	nonpathogenic surrogates	169:192	ABSTRACT Microbial challenge studies using nonpathogenic surrogates provide a practical means for validating thermally based pathogen controls for low-moisture foods.					
36173901	2	7	theme	skim	610:613	arg1	LSMP					628:631	LSMP	628:631	LSMP	628:631	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	7	theme	skim	610:613	arg1	powder					620:625	lactose-free skim milk powder	597:625	lactose-free skim milk powder (LSMP)	597:632	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	8	theme	faecium	562:568	arg1	resistance					507:516	relative thermal resistance	490:516	relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP)	490:689	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	7	9	theme	Estimated	1323:1331	arg1	D90°C-values					1333:1344	Estimated D90°C-values	1323:1344	Estimated D90°C-values	1323:1344	Estimated D90°C-values were approximately two times higher for E. faecium (P < 0.05) than for Salmonella in SMP, LP, and MPI, but statistically similar (P > 0.05) in LSMP.					
36173901	6	10	theme	elevated	1249:1256	arg1	temperatures					1258:1269	elevated temperatures	1249:1269	elevated temperatures	1249:1269	The aw changes and glass transition temperature were assessed at elevated temperatures by using uninoculated, equilibrated powder samples.					
36173901	7	11	dep	similar	1467:1473	arg1	>					1478:1478	P > 0.05	1476:1483	P > 0.05	1476:1483	Estimated D90°C-values were approximately two times higher for E. faecium (P < 0.05) than for Salmonella in SMP, LP, and MPI, but statistically similar (P > 0.05) in LSMP.					
36173901	2	12	theme	thermal	314:320	arg1	resistance					322:331	the relative thermal resistance	301:331	the relative thermal resistance	301:331	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	1	13	theme	practical	204:212	arg1	means					214:218	a practical means	202:218	a practical means for validating thermally based pathogen controls for low-moisture foods	202:290	ABSTRACT Microbial challenge studies using nonpathogenic surrogates provide a practical means for validating thermally based pathogen controls for low-moisture foods.					
36173901	9	14	theme	transition	1794:1803	arg1	temperature					1805:1815	the glass transition temperature	1784:1815	the glass transition temperature observed only for SMP and MPI	1784:1845	Thermally induced changes in aw were seen only for LP and MPI, with the glass transition temperature observed only for SMP and MPI.					
36173901	2	15	from	powder	679:684	arg1	resistance					507:516	relative thermal resistance	490:516	relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP)	490:689	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	0	16	from	Resistance	46:55	arg1	Powders					117:123	Dairy Powders	111:123	Dairy Powders	111:123	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.					
36173901	2	17	dep	resistance	322:331	arg1	kill					337:340	kill	337:340	kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella	337:425	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	4	18	dep	mixed	900:904	arg1	preequilibrated					907:921	preequilibrated	907:921	preequilibrated	907:921	Dairy powders were inoculated with agar-grown cultures, mixed, preequilibrated at 0.25 water activity (aw), ground to achieve homogeneity, reequilibrated, and subjected to isothermal treatment.					
36173901	4	18	dep	mixed	900:904	arg1	ground					952:957	ground	952:957	ground	952:957	Dairy powders were inoculated with agar-grown cultures, mixed, preequilibrated at 0.25 water activity (aw), ground to achieve homogeneity, reequilibrated, and subjected to isothermal treatment.					
36173901	7	19	dep	times	1369:1373	arg1	higher					1375:1380	higher	1375:1380	higher	1375:1380	Estimated D90°C-values were approximately two times higher for E. faecium (P < 0.05) than for Salmonella in SMP, LP, and MPI, but statistically similar (P > 0.05) in LSMP.					
36173901	3	20	theme	survivors	789:797	arg1	resuscitation					762:774	resuscitation	762:774	resuscitation of bacterial survivors	762:797	The impact of sugar composition (lactose versus glucose-galactose) on resuscitation of bacterial survivors, by using SMP and LSMP, was also determined.					
36173901	2	21	theme	skim	573:576	arg1	milk					578:581	skim milk	573:581	skim milk powder (SMP)	573:594	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	6	22	theme	glass	1203:1207	arg1	temperature					1220:1230	glass transition temperature	1203:1230	glass transition temperature	1203:1230	The aw changes and glass transition temperature were assessed at elevated temperatures by using uninoculated, equilibrated powder samples.					
36173901	2	23	theme	lactose	671:677	arg1	LP					687:688	LP	687:688	LP	687:688	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	23	theme	lactose	671:677	arg1	powder					679:684	lactose powder	671:684	lactose powder (LP)	671:689	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	3	24	theme	composition	712:722	arg1	impact					696:701	The impact	692:701	The impact of sugar composition (lactose versus glucose-galactose) on resuscitation of bacterial survivors	692:797	The impact of sugar composition (lactose versus glucose-galactose) on resuscitation of bacterial survivors, by using SMP and LSMP, was also determined.					
36173901	8	25	theme	sugars	1507:1512	arg1	Addition					1495:1502	Addition	1495:1502	Addition of sugars to recovery media	1495:1530	Addition of sugars to recovery media did not influence survivor resuscitation from heat-treated SMP and LSMP, confirming that microbial inactivation was impacted primarily by the thermal treatment, not the recovery step.					
36173901	8	26	theme	thermal	1674:1680	arg1	treatment					1682:1690	the thermal treatment	1670:1690	the thermal treatment	1670:1690	Addition of sugars to recovery media did not influence survivor resuscitation from heat-treated SMP and LSMP, confirming that microbial inactivation was impacted primarily by the thermal treatment, not the recovery step.					
36173901	1	27	theme	Microbial	135:143	arg1	studies					155:161	ABSTRACT Microbial challenge studies	126:161	ABSTRACT Microbial challenge studies using nonpathogenic surrogates	126:192	ABSTRACT Microbial challenge studies using nonpathogenic surrogates provide a practical means for validating thermally based pathogen controls for low-moisture foods.					
36173901	2	28	theme	relative	490:497	arg1	resistance					507:516	relative thermal resistance	490:516	relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP)	490:689	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	5	29	theme	global	1165:1170	arg1	regression					1172:1181	one-step global regression	1156:1181	one-step global regression	1156:1181	After enumeration on nonselective differential media, log-linear and Bigelow models were fit to the survivor data via one-step global regression.					
36173901	10	30	theme	greater	1892:1898	arg1	lethality					1900:1908	greater lethality	1892:1908	greater lethality of E. faecium than Salmonella	1892:1938	In conclusion, rather than always requiring greater lethality of E. faecium than Salmonella, these findings suggest that sufficient pathogen controls for low-moisture foods can also be validated by thoroughly documenting the appropriate kill ratios of E. faecium to Salmonella.					
36173901	0	31	theme	Enterococcus	60:71	arg1	B-2354					86:91	Enterococcus faecium NRRL B-2354	60:91	Enterococcus faecium NRRL B-2354	60:91	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.					
36173901	10	32	dep	ratios	2090:2095	arg1	kill					2085:2088	kill	2085:2088	kill	2085:2088	In conclusion, rather than always requiring greater lethality of E. faecium than Salmonella, these findings suggest that sufficient pathogen controls for low-moisture foods can also be validated by thoroughly documenting the appropriate kill ratios of E. faecium to Salmonella.					
36173901	2	33	theme	Salmonella	535:544	arg1	cocktail					546:553	a five-strain Salmonella cocktail	521:553	a five-strain Salmonella cocktail	521:553	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	0	34	from	Effect	0:5	arg1	Resistance					46:55	Thermal Resistance	38:55	Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders	38:123	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.					
36173901	0	35	theme	NRRL	81:84	arg1	B-2354					86:91	Enterococcus faecium NRRL B-2354	60:91	Enterococcus faecium NRRL B-2354	60:91	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.					
36173901	4	36	theme	agar-grown	879:888	arg1	cultures					890:897	agar-grown cultures	879:897	agar-grown cultures	879:897	Dairy powders were inoculated with agar-grown cultures, mixed, preequilibrated at 0.25 water activity (aw), ground to achieve homogeneity, reequilibrated, and subjected to isothermal treatment.					
36173901	2	37	theme	food	452:455	arg1	composition					457:467	food composition	452:467	food composition	452:467	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	5	38	theme	Bigelow	1107:1113	arg1	models					1115:1120	log-linear and Bigelow models	1092:1120	models	1115:1120	After enumeration on nonselective differential media, log-linear and Bigelow models were fit to the survivor data via one-step global regression.					
36173901	2	39	theme	milk	639:642	arg1	MPI					661:663	MPI	661:663	MPI	661:663	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	39	theme	milk	639:642	arg1	isolate					652:658	90% milk protein isolate	635:658	90% milk protein isolate (MPI)	635:664	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	0	40	dep	Enterococcus	60:71	arg1	faecium					73:79	faecium	73:79	faecium	73:79	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.					
36173901	2	41	theme	90	635:636	arg1	%					637:637	%	637:637	%	637:637	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	0	42	theme	Lactose	10:16	arg1	Effect					0:5	Effect	0:5	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.	0:124	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.					
36173901	9	43	theme	glass	1788:1792	arg1	transition					1794:1803	the glass transition	1784:1803	the glass transition temperature observed only for SMP and MPI	1784:1845	Thermally induced changes in aw were seen only for LP and MPI, with the glass transition temperature observed only for SMP and MPI.					
36173901	5	44	from	enumeration	1044:1054	arg1	media					1085:1089	nonselective differential media	1059:1089	nonselective differential media	1059:1089	After enumeration on nonselective differential media, log-linear and Bigelow models were fit to the survivor data via one-step global regression.					
36173901	0	45	theme	Milk	22:25	arg1	Protein					27:33	Milk Protein	22:33	Milk Protein	22:33	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.					
36173901	4	46	theme	water	931:935	arg1	aw					947:948	aw	947:948	aw	947:948	Dairy powders were inoculated with agar-grown cultures, mixed, preequilibrated at 0.25 water activity (aw), ground to achieve homogeneity, reequilibrated, and subjected to isothermal treatment.					
36173901	4	46	theme	water	931:935	arg1	activity					937:944	0.25 water activity	926:944	0.25 water activity (aw)	926:949	Dairy powders were inoculated with agar-grown cultures, mixed, preequilibrated at 0.25 water activity (aw), ground to achieve homogeneity, reequilibrated, and subjected to isothermal treatment.					
36173901	5	47	theme	differential	1072:1083	arg1	media					1085:1089	nonselective differential media	1059:1089	nonselective differential media	1059:1089	After enumeration on nonselective differential media, log-linear and Bigelow models were fit to the survivor data via one-step global regression.					
36173901	9	48	theme	induced	1726:1732	arg1	changes					1734:1740	Thermally induced changes	1716:1740	Thermally induced changes in aw	1716:1746	Thermally induced changes in aw were seen only for LP and MPI, with the glass transition temperature observed only for SMP and MPI.					
36173901	2	49	from	cocktail	546:553	arg1	LSMP					628:631	LSMP	628:631	LSMP	628:631	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	49	from	cocktail	546:553	arg1	powder					679:684	lactose powder	671:684	lactose powder (LP)	671:689	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	49	from	cocktail	546:553	arg1	isolate					652:658	90% milk protein isolate	635:658	90% milk protein isolate (MPI)	635:664	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	49	from	cocktail	546:553	arg1	powder					583:588	skim milk powder	573:588	skim milk powder (SMP)	573:594	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	49	from	cocktail	546:553	arg1	MPI					661:663	MPI	661:663	MPI	661:663	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	49	from	cocktail	546:553	arg1	LP					687:688	LP	687:688	LP	687:688	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	49	from	cocktail	546:553	arg1	powder					620:625	lactose-free skim milk powder	597:625	lactose-free skim milk powder (LSMP)	597:632	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	49	from	cocktail	546:553	arg1	SMP					591:593	SMP	591:593	SMP	591:593	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	50	theme	B-2354	378:383	arg1	ratio					342:346	ratio	342:346	ratio	342:346	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	1	51	theme	based	245:249	arg1	controls					260:267	thermally based pathogen controls	235:267	thermally based pathogen controls for low-moisture foods	235:290	ABSTRACT Microbial challenge studies using nonpathogenic surrogates provide a practical means for validating thermally based pathogen controls for low-moisture foods.					
36173901	6	52	theme	aw	1188:1189	arg1	changes					1191:1197	The aw changes	1184:1197	The aw changes	1184:1197	The aw changes and glass transition temperature were assessed at elevated temperatures by using uninoculated, equilibrated powder samples.					
36173901	2	53	theme	relative	305:312	arg1	resistance					322:331	the relative thermal resistance	301:331	the relative thermal resistance	301:331	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	8	54	theme	survivor	1550:1557	arg1	resuscitation					1559:1571	survivor resuscitation	1550:1571	survivor resuscitation from heat-treated SMP and LSMP	1550:1602	Addition of sugars to recovery media did not influence survivor resuscitation from heat-treated SMP and LSMP, confirming that microbial inactivation was impacted primarily by the thermal treatment, not the recovery step.					
36173901	1	55	theme	low-moisture	273:284	arg1	foods					286:290	low-moisture foods	273:290	low-moisture foods	273:290	ABSTRACT Microbial challenge studies using nonpathogenic surrogates provide a practical means for validating thermally based pathogen controls for low-moisture foods.					
36173901	10	56	theme	sufficient	1969:1978	arg1	controls					1989:1996	sufficient pathogen controls	1969:1996	sufficient pathogen controls for low-moisture foods	1969:2019	In conclusion, rather than always requiring greater lethality of E. faecium than Salmonella, these findings suggest that sufficient pathogen controls for low-moisture foods can also be validated by thoroughly documenting the appropriate kill ratios of E. faecium to Salmonella.					
36173901	2	57	theme	milk	615:618	arg1	LSMP					628:631	LSMP	628:631	LSMP	628:631	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	57	theme	milk	615:618	arg1	powder					620:625	lactose-free skim milk powder	597:625	lactose-free skim milk powder (LSMP)	597:632	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	58	from	isolate	652:658	arg1	resistance					507:516	relative thermal resistance	490:516	relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP)	490:689	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	6	59	theme	equilibrated	1294:1305	arg1	samples					1314:1320	equilibrated powder samples	1294:1320	equilibrated powder samples	1294:1320	The aw changes and glass transition temperature were assessed at elevated temperatures by using uninoculated, equilibrated powder samples.					
36173901	2	60	theme	lactose-free	597:608	arg1	LSMP					628:631	LSMP	628:631	LSMP	628:631	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	60	theme	lactose-free	597:608	arg1	powder					620:625	lactose-free skim milk powder	597:625	lactose-free skim milk powder (LSMP)	597:632	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	10	61	theme	low-moisture	2002:2013	arg1	foods					2015:2019	low-moisture foods	2002:2019	low-moisture foods	2002:2019	In conclusion, rather than always requiring greater lethality of E. faecium than Salmonella, these findings suggest that sufficient pathogen controls for low-moisture foods can also be validated by thoroughly documenting the appropriate kill ratios of E. faecium to Salmonella.					
36173901	0	62	theme	Dairy	111:115	arg1	Powders					117:123	Dairy Powders	111:123	Dairy Powders	111:123	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.					
36173901	9	63	from	changes	1734:1740	arg1	aw					1745:1746	aw	1745:1746	aw	1745:1746	Thermally induced changes in aw were seen only for LP and MPI, with the glass transition temperature observed only for SMP and MPI.					
36173901	4	64	theme	isothermal	1016:1025	arg1	treatment					1027:1035	isothermal treatment	1016:1035	isothermal treatment	1016:1035	Dairy powders were inoculated with agar-grown cultures, mixed, preequilibrated at 0.25 water activity (aw), ground to achieve homogeneity, reequilibrated, and subjected to isothermal treatment.					
36173901	10	65	theme	faecium	1916:1922	arg1	lethality					1900:1908	greater lethality	1892:1908	greater lethality of E. faecium than Salmonella	1892:1938	In conclusion, rather than always requiring greater lethality of E. faecium than Salmonella, these findings suggest that sufficient pathogen controls for low-moisture foods can also be validated by thoroughly documenting the appropriate kill ratios of E. faecium to Salmonella.					
36173901	2	66	theme	cocktail	546:553	arg1	resistance					507:516	relative thermal resistance	490:516	relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP)	490:689	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	67	from	resistance	507:516	arg1	LSMP					628:631	LSMP	628:631	LSMP	628:631	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	67	from	resistance	507:516	arg1	powder					679:684	lactose powder	671:684	lactose powder (LP)	671:689	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	67	from	resistance	507:516	arg1	isolate					652:658	90% milk protein isolate	635:658	90% milk protein isolate (MPI)	635:664	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	67	from	resistance	507:516	arg1	powder					583:588	skim milk powder	573:588	skim milk powder (SMP)	573:594	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	67	from	resistance	507:516	arg1	MPI					661:663	MPI	661:663	MPI	661:663	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	67	from	resistance	507:516	arg1	LP					687:688	LP	687:688	LP	687:688	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	67	from	resistance	507:516	arg1	powder					620:625	lactose-free skim milk powder	597:625	lactose-free skim milk powder (LSMP)	597:632	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	67	from	resistance	507:516	arg1	SMP					591:593	SMP	591:593	SMP	591:593	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	3	68	theme	sugar	706:710	arg1	composition					712:722	sugar composition	706:722	sugar composition (lactose versus glucose-galactose)	706:757	The impact of sugar composition (lactose versus glucose-galactose) on resuscitation of bacterial survivors, by using SMP and LSMP, was also determined.					
36173901	8	69	theme	microbial	1621:1629	arg1	inactivation					1631:1642	microbial inactivation	1621:1642	microbial inactivation	1621:1642	Addition of sugars to recovery media did not influence survivor resuscitation from heat-treated SMP and LSMP, confirming that microbial inactivation was impacted primarily by the thermal treatment, not the recovery step.					
36173901	2	70	theme	milk	578:581	arg1	SMP					591:593	SMP	591:593	SMP	591:593	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	70	theme	milk	578:581	arg1	powder					583:588	skim milk powder	573:588	skim milk powder (SMP)	573:594	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	6	71	theme	transition	1209:1218	arg1	temperature					1220:1230	glass transition temperature	1203:1230	glass transition temperature	1203:1230	The aw changes and glass transition temperature were assessed at elevated temperatures by using uninoculated, equilibrated powder samples.					
36173901	7	72	theme	P	1476:1476	arg1	>					1478:1478	P > 0.05	1476:1483	P > 0.05	1476:1483	Estimated D90°C-values were approximately two times higher for E. faecium (P < 0.05) than for Salmonella in SMP, LP, and MPI, but statistically similar (P > 0.05) in LSMP.					
36173901	1	73	theme	ABSTRACT	126:133	arg1	studies					155:161	ABSTRACT Microbial challenge studies	126:161	ABSTRACT Microbial challenge studies using nonpathogenic surrogates	126:192	ABSTRACT Microbial challenge studies using nonpathogenic surrogates provide a practical means for validating thermally based pathogen controls for low-moisture foods.					
36173901	3	74	theme	bacterial	779:787	arg1	survivors					789:797	bacterial survivors	779:797	bacterial survivors	779:797	The impact of sugar composition (lactose versus glucose-galactose) on resuscitation of bacterial survivors, by using SMP and LSMP, was also determined.					
36173901	2	75	theme	thermal	499:505	arg1	resistance					507:516	relative thermal resistance	490:516	relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP)	490:689	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	5	76	theme	one-step	1156:1163	arg1	regression					1172:1181	one-step global regression	1156:1181	one-step global regression	1156:1181	After enumeration on nonselective differential media, log-linear and Bigelow models were fit to the survivor data via one-step global regression.					
36173901	6	77	theme	uninoculated	1280:1291	arg1	samples					1314:1320	equilibrated powder samples	1294:1320	equilibrated powder samples	1294:1320	The aw changes and glass transition temperature were assessed at elevated temperatures by using uninoculated, equilibrated powder samples.					
36173901	6	78	theme	powder	1307:1312	arg1	samples					1314:1320	equilibrated powder samples	1294:1320	equilibrated powder samples	1294:1320	The aw changes and glass transition temperature were assessed at elevated temperatures by using uninoculated, equilibrated powder samples.					
36173901	1	79	theme	challenge	145:153	arg1	studies					155:161	ABSTRACT Microbial challenge studies	126:161	ABSTRACT Microbial challenge studies using nonpathogenic surrogates	126:192	ABSTRACT Microbial challenge studies using nonpathogenic surrogates provide a practical means for validating thermally based pathogen controls for low-moisture foods.					
36173901	7	80	from	times	1369:1373	arg1	LSMP					1489:1492	LSMP	1489:1492	LSMP	1489:1492	Estimated D90°C-values were approximately two times higher for E. faecium (P < 0.05) than for Salmonella in SMP, LP, and MPI, but statistically similar (P > 0.05) in LSMP.					
36173901	4	81	theme	mixed	900:904	arg1	cultures					890:897	agar-grown cultures	879:897	agar-grown cultures	879:897	Dairy powders were inoculated with agar-grown cultures, mixed, preequilibrated at 0.25 water activity (aw), ground to achieve homogeneity, reequilibrated, and subjected to isothermal treatment.					
36173901	3	82	from	impact	696:701	arg1	resuscitation					762:774	resuscitation	762:774	resuscitation of bacterial survivors	762:797	The impact of sugar composition (lactose versus glucose-galactose) on resuscitation of bacterial survivors, by using SMP and LSMP, was also determined.					
36173901	3	83	dep	composition	712:722	arg1	lactose					725:731	lactose	725:731	lactose	725:731	The impact of sugar composition (lactose versus glucose-galactose) on resuscitation of bacterial survivors, by using SMP and LSMP, was also determined.					
36173901	3	83	dep	composition	712:722	arg1	glucose-galactose					740:756	glucose-galactose	740:756	glucose-galactose	740:756	The impact of sugar composition (lactose versus glucose-galactose) on resuscitation of bacterial survivors, by using SMP and LSMP, was also determined.					
36173901	0	84	theme	B-2354	86:91	arg1	Resistance					46:55	Thermal Resistance	38:55	Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders	38:123	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.					
36173901	2	85	theme	five-strain	523:533	arg1	cocktail					546:553	a five-strain Salmonella cocktail	521:553	a five-strain Salmonella cocktail	521:553	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	10	86	theme	appropriate	2073:2083	arg1	ratios					2090:2095	the appropriate kill ratios	2069:2095	the appropriate kill ratios of E. faecium to Salmonella	2069:2123	In conclusion, rather than always requiring greater lethality of E. faecium than Salmonella, these findings suggest that sufficient pathogen controls for low-moisture foods can also be validated by thoroughly documenting the appropriate kill ratios of E. faecium to Salmonella.					
36173901	2	87	dep	Enterococcus	352:363	arg1	faecium					365:371	faecium	365:371	faecium	365:371	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	0	88	theme	Salmonella	97:106	arg1	Resistance					46:55	Thermal Resistance	38:55	Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders	38:123	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.					
36173901	2	89	theme	protein	644:650	arg1	MPI					661:663	MPI	661:663	MPI	661:663	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	89	theme	protein	644:650	arg1	isolate					652:658	90% milk protein isolate	635:658	90% milk protein isolate (MPI)	635:664	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	90	theme	nonpathogenic	388:400	arg1	surrogate					402:410	a nonpathogenic surrogate	386:410	a nonpathogenic surrogate	386:410	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	90	theme	nonpathogenic	388:400	arg1	B-2354					378:383	Enterococcus faecium NRRL B-2354	352:383	Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate)	352:411	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	8	91	theme	recovery	1701:1708	arg1	step					1710:1713	the recovery step	1697:1713	the recovery step	1697:1713	Addition of sugars to recovery media did not influence survivor resuscitation from heat-treated SMP and LSMP, confirming that microbial inactivation was impacted primarily by the thermal treatment, not the recovery step.					
36173901	2	92	theme	%	637:637	arg1	MPI					661:663	MPI	661:663	MPI	661:663	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	92	theme	%	637:637	arg1	isolate					652:658	90% milk protein isolate	635:658	90% milk protein isolate (MPI)	635:664	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	5	93	theme	log-linear	1092:1101	arg1	models					1115:1120	log-linear and Bigelow models	1092:1120	models	1115:1120	After enumeration on nonselective differential media, log-linear and Bigelow models were fit to the survivor data via one-step global regression.					
36173901	10	94	theme	pathogen	1980:1987	arg1	controls					1989:1996	sufficient pathogen controls	1969:1996	sufficient pathogen controls for low-moisture foods	1969:2019	In conclusion, rather than always requiring greater lethality of E. faecium than Salmonella, these findings suggest that sufficient pathogen controls for low-moisture foods can also be validated by thoroughly documenting the appropriate kill ratios of E. faecium to Salmonella.					
36173901	0	95	theme	Protein	27:33	arg1	Effect					0:5	Effect	0:5	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.	0:124	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.					
36173901	4	96	from	activity	937:944	arg1	preequilibrated					907:921	preequilibrated	907:921	preequilibrated	907:921	Dairy powders were inoculated with agar-grown cultures, mixed, preequilibrated at 0.25 water activity (aw), ground to achieve homogeneity, reequilibrated, and subjected to isothermal treatment.					
36173901	8	97	from	SMP	1591:1593	arg1	resuscitation					1559:1571	survivor resuscitation	1550:1571	survivor resuscitation from heat-treated SMP and LSMP	1550:1602	Addition of sugars to recovery media did not influence survivor resuscitation from heat-treated SMP and LSMP, confirming that microbial inactivation was impacted primarily by the thermal treatment, not the recovery step.					
36173901	0	98	theme	Thermal	38:44	arg1	Resistance					46:55	Thermal Resistance	38:55	Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders	38:123	Effect of Lactose and Milk Protein on Thermal Resistance of Enterococcus faecium NRRL B-2354 and Salmonella in Dairy Powders.					
36173901	5	99	theme	nonselective	1059:1070	arg1	media					1085:1089	nonselective differential media	1059:1089	nonselective differential media	1059:1089	After enumeration on nonselective differential media, log-linear and Bigelow models were fit to the survivor data via one-step global regression.					
36173901	4	100	theme	Dairy	844:848	arg1	powders					850:856	Dairy powders	844:856	Dairy powders	844:856	Dairy powders were inoculated with agar-grown cultures, mixed, preequilibrated at 0.25 water activity (aw), ground to achieve homogeneity, reequilibrated, and subjected to isothermal treatment.					
36173901	2	101	from	powder	620:625	arg1	resistance					507:516	relative thermal resistance	490:516	relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP)	490:689	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	8	102	theme	recovery	1517:1524	arg1	media					1526:1530	recovery media	1517:1530	recovery media	1517:1530	Addition of sugars to recovery media did not influence survivor resuscitation from heat-treated SMP and LSMP, confirming that microbial inactivation was impacted primarily by the thermal treatment, not the recovery step.					
36173901	5	103	theme	survivor	1138:1145	arg1	data					1147:1150	the survivor data	1134:1150	the survivor data	1134:1150	After enumeration on nonselective differential media, log-linear and Bigelow models were fit to the survivor data via one-step global regression.					
36173901	8	104	from	LSMP	1599:1602	arg1	resuscitation					1559:1571	survivor resuscitation	1550:1571	survivor resuscitation from heat-treated SMP and LSMP	1550:1602	Addition of sugars to recovery media did not influence survivor resuscitation from heat-treated SMP and LSMP, confirming that microbial inactivation was impacted primarily by the thermal treatment, not the recovery step.					
36173901	2	105	theme	NRRL	373:376	arg1	surrogate					402:410	a nonpathogenic surrogate	386:410	a nonpathogenic surrogate	386:410	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	2	105	theme	NRRL	373:376	arg1	B-2354					378:383	Enterococcus faecium NRRL B-2354	352:383	Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate)	352:411	Because the relative thermal resistance, or kill ratio, of Enterococcus faecium NRRL B-2354 (a nonpathogenic surrogate) to Salmonella is greatly influenced by food composition, this study assessed relative thermal resistance of a five-strain Salmonella cocktail and E. faecium in skim milk powder (SMP), lactose-free skim milk powder (LSMP), 90% milk protein isolate (MPI), and lactose powder (LP).					
36173901	1	106	theme	pathogen	251:258	arg1	controls					260:267	thermally based pathogen controls	235:267	thermally based pathogen controls for low-moisture foods	235:290	ABSTRACT Microbial challenge studies using nonpathogenic surrogates provide a practical means for validating thermally based pathogen controls for low-moisture foods.					
36235081	2	0	theme	microwave	444:452	arg1	power					454:458	microwave power	444:458	microwave power (180, 300, and 450 W)	444:480	In this study, three independent variables thought to contribute to microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%).					
36235081	2	0	theme	microwave	444:452	arg1	W					479:479	180, 300, and 450 W	461:479	180, 300, and 450 W	461:479	In this study, three independent variables thought to contribute to microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%).					
36235081	3	1	theme	central	581:587	arg1	design					599:604	central composite design	581:604	central composite design (CCD)	581:610	Response surface methodology was applied using central composite design (CCD) to examine the effect of independent variables on the parameter measured.					
36235081	3	1	theme	central	581:587	arg1	CCD					607:609	CCD	607:609	CCD	607:609	Response surface methodology was applied using central composite design (CCD) to examine the effect of independent variables on the parameter measured.					
36235081	3	2	from	effect	627:632	arg1	parameter					666:674	the parameter	662:674	the parameter measured	662:683	Response surface methodology was applied using central composite design (CCD) to examine the effect of independent variables on the parameter measured.					
36235081	8	3	theme	%	1568:1568	arg1	content					1616:1622	methoxyl content	1607:1622	methoxyl content of 6.07%	1607:1631	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	3	theme	%	1568:1568	arg1	yield					1554:1558	a yield	1552:1558	a yield of 21.10%	1552:1568	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	3	theme	%	1568:1568	arg1	weight					1582:1587	equivalent weight	1571:1587	equivalent weight of 602.40 mg/mol	1571:1604	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	3	theme	%	1568:1568	arg1	values					1508:1513	Other parameter values	1492:1513	Other parameter values measured under these conditions	1492:1545	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	3	theme	%	1568:1568	arg1	content					1656:1662	galacturonic acid content	1638:1662	galacturonic acid content of 72.86%	1638:1672	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	2	4	theme	microwave-assisted	350:367	arg1	extraction					376:385	microwave-assisted pectin extraction	350:385	microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%)	350:531	In this study, three independent variables thought to contribute to microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%).					
36235081	4	5	theme	moisture	751:758	arg1	content					760:766	moisture content	751:766	moisture content	751:766	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	4	5	theme	moisture	751:758	arg1	parameters					702:711	the seven parameters	692:711	the seven parameters measured	692:720	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	6	6	dep	results	1116:1122	arg1	concluded					1124:1132	concluded	1124:1132	results concluded that the factors that had the most significant effect on temperature, moisture content, and ash content were extraction time and microwave power	1116:1277	Optimization results concluded that the factors that had the most significant effect on temperature, moisture content, and ash content were extraction time and microwave power.					
36235081	4	7	theme	galacturonic	823:834	arg1	content					841:847	galacturonic acid content	823:847	galacturonic acid content	823:847	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	4	7	theme	galacturonic	823:834	arg1	parameters					702:711	the seven parameters	692:711	the seven parameters measured	692:720	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	9	8	theme	pharmaceutical	1798:1811	arg1	industries					1813:1822	the food and pharmaceutical industries	1785:1822	the food and pharmaceutical industries	1785:1822	Pectin extracted from cocoa pod husk using this method has the potential to be further applied, especially in the food and pharmaceutical industries.					
36235081	1	9	theme	independent	155:165	arg1	variables					167:175	independent variables	155:175	independent variables that affect the quality of pectin obtained and determine the best conditions for the extraction process	155:279	Optimization of pectin extraction from cocoa pod husk was carried out to examine independent variables that affect the quality of pectin obtained and determine the best conditions for the extraction process.					
36235081	7	10	theme	%	1489:1489	arg1	content					1477:1483	ash content	1473:1483	ash content of 8%	1473:1489	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	7	10	theme	%	1489:1489	arg1	°C					1441:1442	104 °C	1437:1442	104 °C	1437:1442	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	7	10	theme	%	1489:1489	arg1	content					1454:1460	moisture content	1445:1460	moisture content of 6%	1445:1466	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	5	11	theme	conditions	1046:1055	arg1	optimization					1030:1041	the optimization	1026:1041	the optimization of conditions	1026:1055	Therefore, the optimization of conditions was only reviewed for these three parameters.					
36235081	7	12	theme	°C	1441:1442	arg1	temperature					1422:1432	a temperature	1420:1432	a temperature of 104 °C, moisture content of 6%, and ash content of 8%	1420:1489	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	7	13	theme	30	1329:1330	arg1	min					1332:1334	min	1332:1334	min	1332:1334	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	8	14	theme	equivalent	1571:1580	arg1	weight					1582:1587	equivalent weight	1571:1587	equivalent weight of 602.40 mg/mol	1571:1604	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	7	15	theme	Optimal	1280:1286	arg1	conditions					1288:1297	Optimal conditions	1280:1297	Optimal conditions for pectin extraction	1280:1319	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	1	16	theme	cocoa	113:117	arg1	husk					123:126	cocoa pod husk	113:126	cocoa pod husk	113:126	Optimization of pectin extraction from cocoa pod husk was carried out to examine independent variables that affect the quality of pectin obtained and determine the best conditions for the extraction process.					
36235081	7	17	theme	10	1380:1381	arg1	%					1382:1382	%	1382:1382	%	1382:1382	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	2	18	theme	independent	303:313	arg1	variables					315:323	three independent variables	297:323	three independent variables	297:323	In this study, three independent variables thought to contribute to microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%).					
36235081	0	19	from	Pod	64:66	arg1	Optimization					0:11	Optimization	0:11	Optimization of Microwave-Assisted Pectin Extraction from Cocoa Pod	0:66	Optimization of Microwave-Assisted Pectin Extraction from Cocoa Pod Husk.					
36235081	8	20	theme	%	1631:1631	arg1	content					1616:1622	methoxyl content	1607:1622	methoxyl content of 6.07%	1607:1631	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	20	theme	%	1631:1631	arg1	yield					1554:1558	a yield	1552:1558	a yield of 21.10%	1552:1568	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	20	theme	%	1631:1631	arg1	weight					1582:1587	equivalent weight	1571:1587	equivalent weight of 602.40 mg/mol	1571:1604	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	20	theme	%	1631:1631	arg1	values					1508:1513	Other parameter values	1492:1513	Other parameter values measured under these conditions	1492:1545	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	20	theme	%	1631:1631	arg1	content					1656:1662	galacturonic acid content	1638:1662	galacturonic acid content of 72.86%	1638:1672	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	7	21	theme	min	1332:1334	arg1	time					1347:1350	30 min extraction time	1329:1350	30 min extraction time	1329:1350	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	4	22	theme	moisture	980:987	arg1	content					989:995	moisture content	980:995	moisture content	980:995	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	1	23	theme	extraction	262:271	arg1	process					273:279	the extraction process	258:279	the extraction process	258:279	Optimization of pectin extraction from cocoa pod husk was carried out to examine independent variables that affect the quality of pectin obtained and determine the best conditions for the extraction process.					
36235081	6	24	theme	moisture	1204:1211	arg1	content					1213:1219	moisture content	1204:1219	moisture content	1204:1219	Optimization results concluded that the factors that had the most significant effect on temperature, moisture content, and ash content were extraction time and microwave power.					
36235081	8	25	theme	galacturonic	1638:1649	arg1	content					1656:1662	galacturonic acid content	1638:1662	galacturonic acid content of 72.86%	1638:1672	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	6	26	contain	had	1156:1158	arg2	effect					1181:1186	the most significant effect	1160:1186	the most significant effect	1160:1186	Optimization results concluded that the factors that had the most significant effect on temperature, moisture content, and ash content were extraction time and microwave power.					
36235081	6	26	contain	had	1156:1158	arg1	time					1254:1257	extraction time	1243:1257	extraction time	1243:1257	Optimization results concluded that the factors that had the most significant effect on temperature, moisture content, and ash content were extraction time and microwave power.					
36235081	6	26	contain	had	1156:1158	arg1	factors					1143:1149	the factors	1139:1149	the factors that had the most significant effect on temperature, moisture content, and ash content	1139:1236	Optimization results concluded that the factors that had the most significant effect on temperature, moisture content, and ash content were extraction time and microwave power.					
36235081	6	27	theme	ash	1226:1228	arg1	content					1230:1236	ash content	1226:1236	ash content	1226:1236	Optimization results concluded that the factors that had the most significant effect on temperature, moisture content, and ash content were extraction time and microwave power.					
36235081	4	28	theme	methoxyl	801:808	arg1	parameters					702:711	the seven parameters	692:711	the seven parameters measured	692:720	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	4	28	theme	methoxyl	801:808	arg1	content					810:816	methoxyl content	801:816	methoxyl content	801:816	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	3	29	theme	composite	589:597	arg1	design					599:604	central composite design	581:604	central composite design (CCD)	581:610	Response surface methodology was applied using central composite design (CCD) to examine the effect of independent variables on the parameter measured.					
36235081	3	29	theme	composite	589:597	arg1	CCD					607:609	CCD	607:609	CCD	607:609	Response surface methodology was applied using central composite design (CCD) to examine the effect of independent variables on the parameter measured.					
36235081	7	30	theme	solvent	1384:1390	arg1	concentration					1392:1404	10% solvent concentration	1380:1404	10% solvent concentration	1380:1404	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	7	31	theme	ash	1473:1475	arg1	content					1477:1483	ash content	1473:1483	ash content of 8%	1473:1489	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	7	32	theme	pectin	1303:1308	arg1	extraction					1310:1319	pectin extraction	1303:1319	pectin extraction	1303:1319	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	8	33	theme	%	1672:1672	arg1	content					1616:1622	methoxyl content	1607:1622	methoxyl content of 6.07%	1607:1631	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	33	theme	%	1672:1672	arg1	yield					1554:1558	a yield	1552:1558	a yield of 21.10%	1552:1568	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	33	theme	%	1672:1672	arg1	weight					1582:1587	equivalent weight	1571:1587	equivalent weight of 602.40 mg/mol	1571:1604	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	33	theme	%	1672:1672	arg1	values					1508:1513	Other parameter values	1492:1513	Other parameter values measured under these conditions	1492:1545	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	33	theme	%	1672:1672	arg1	content					1656:1662	galacturonic acid content	1638:1662	galacturonic acid content of 72.86%	1638:1672	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	1	34	theme	pectin	90:95	arg1	extraction					97:106	pectin extraction	90:106	pectin extraction from cocoa pod husk	90:126	Optimization of pectin extraction from cocoa pod husk was carried out to examine independent variables that affect the quality of pectin obtained and determine the best conditions for the extraction process.					
36235081	7	35	theme	content	1477:1483	arg1	temperature					1422:1432	a temperature	1420:1432	a temperature of 104 °C, moisture content of 6%, and ash content of 8%	1420:1489	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	7	36	theme	450	1353:1355	arg1	W					1357:1357	W	1357:1357	W	1357:1357	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	4	37	theme	analysis	866:873	arg1	test					875:878	the statistical analysis test	850:878	the statistical analysis test	850:878	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	7	38	theme	content	1454:1460	arg1	temperature					1422:1432	a temperature	1420:1432	a temperature of 104 °C, moisture content of 6%, and ash content of 8%	1420:1489	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	8	39	theme	acid	1651:1654	arg1	content					1656:1662	galacturonic acid content	1638:1662	galacturonic acid content of 72.86%	1638:1672	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	7	40	theme	extraction	1336:1345	arg1	time					1347:1350	30 min extraction time	1329:1350	30 min extraction time	1329:1350	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	1	41	theme	extraction	97:106	arg1	Optimization					74:85	Optimization	74:85	Optimization of pectin extraction from cocoa pod husk	74:126	Optimization of pectin extraction from cocoa pod husk was carried out to examine independent variables that affect the quality of pectin obtained and determine the best conditions for the extraction process.					
36235081	0	42	theme	Pectin	35:40	arg1	Extraction					42:51	Microwave-Assisted Pectin Extraction	16:51	Microwave-Assisted Pectin Extraction	16:51	Optimization of Microwave-Assisted Pectin Extraction from Cocoa Pod Husk.					
36235081	8	43	theme	mg/mol	1599:1604	arg1	content					1616:1622	methoxyl content	1607:1622	methoxyl content of 6.07%	1607:1631	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	43	theme	mg/mol	1599:1604	arg1	yield					1554:1558	a yield	1552:1558	a yield of 21.10%	1552:1568	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	43	theme	mg/mol	1599:1604	arg1	weight					1582:1587	equivalent weight	1571:1587	equivalent weight of 602.40 mg/mol	1571:1604	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	43	theme	mg/mol	1599:1604	arg1	values					1508:1513	Other parameter values	1492:1513	Other parameter values measured under these conditions	1492:1545	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	43	theme	mg/mol	1599:1604	arg1	content					1656:1662	galacturonic acid content	1638:1662	galacturonic acid content of 72.86%	1638:1672	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	1	44	from	husk	123:126	arg1	Optimization					74:85	Optimization	74:85	Optimization of pectin extraction from cocoa pod husk	74:126	Optimization of pectin extraction from cocoa pod husk was carried out to examine independent variables that affect the quality of pectin obtained and determine the best conditions for the extraction process.					
36235081	1	44	from	husk	123:126	arg1	extraction					97:106	pectin extraction	90:106	pectin extraction from cocoa pod husk	90:126	Optimization of pectin extraction from cocoa pod husk was carried out to examine independent variables that affect the quality of pectin obtained and determine the best conditions for the extraction process.					
36235081	4	45	theme	response	946:953	arg1	values					955:960	predicting response values	935:960	predicting response values	935:960	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	0	46	theme	Microwave-Assisted	16:33	arg1	Extraction					42:51	Microwave-Assisted Pectin Extraction	16:51	Microwave-Assisted Pectin Extraction	16:51	Optimization of Microwave-Assisted Pectin Extraction from Cocoa Pod Husk.					
36235081	4	47	theme	ash	769:771	arg1	content					773:779	ash content	769:779	ash content	769:779	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	4	47	theme	ash	769:771	arg1	parameters					702:711	the seven parameters	692:711	the seven parameters measured	692:720	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	9	48	theme	food	1789:1792	arg1	industries					1813:1822	the food and pharmaceutical industries	1785:1822	the food and pharmaceutical industries	1785:1822	Pectin extracted from cocoa pod husk using this method has the potential to be further applied, especially in the food and pharmaceutical industries.					
36235081	0	49	dep	Husk	68:71	arg1	Optimization					0:11	Optimization	0:11	Optimization of Microwave-Assisted Pectin Extraction from Cocoa Pod	0:66	Optimization of Microwave-Assisted Pectin Extraction from Cocoa Pod Husk.					
36235081	6	50	theme	microwave	1263:1271	arg1	power					1273:1277	microwave power	1263:1277	microwave power	1263:1277	Optimization results concluded that the factors that had the most significant effect on temperature, moisture content, and ash content were extraction time and microwave power.					
36235081	7	51	theme	moisture	1445:1452	arg1	content					1454:1460	moisture content	1445:1460	moisture content of 6%	1445:1466	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	4	52	theme	equivalent	782:791	arg1	weight					793:798	equivalent weight	782:798	equivalent weight	782:798	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	4	52	theme	equivalent	782:791	arg1	parameters					702:711	the seven parameters	692:711	the seven parameters measured	692:720	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	1	53	theme	pectin	204:209	arg1	quality					193:199	the quality	189:199	the quality of pectin obtained	189:218	Optimization of pectin extraction from cocoa pod husk was carried out to examine independent variables that affect the quality of pectin obtained and determine the best conditions for the extraction process.					
36235081	7	54	theme	%	1466:1466	arg1	content					1477:1483	ash content	1473:1483	ash content of 8%	1473:1489	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	7	54	theme	%	1466:1466	arg1	°C					1441:1442	104 °C	1437:1442	104 °C	1437:1442	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	7	54	theme	%	1466:1466	arg1	content					1454:1460	moisture content	1445:1460	moisture content of 6%	1445:1466	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	2	55	theme	extraction	406:415	arg1	min					438:440	20, 25, and 30 min	423:440	20, 25, and 30 min	423:440	In this study, three independent variables thought to contribute to microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%).					
36235081	2	55	theme	extraction	406:415	arg1	time					417:420	extraction time	406:420	extraction time (20, 25, and 30 min)	406:441	In this study, three independent variables thought to contribute to microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%).					
36235081	0	56	theme	Extraction	42:51	arg1	Optimization					0:11	Optimization	0:11	Optimization of Microwave-Assisted Pectin Extraction from Cocoa Pod	0:66	Optimization of Microwave-Assisted Pectin Extraction from Cocoa Pod Husk.					
36235081	3	57	theme	independent	637:647	arg1	variables					649:657	independent variables	637:657	independent variables	637:657	Response surface methodology was applied using central composite design (CCD) to examine the effect of independent variables on the parameter measured.					
36235081	2	58	dep	concentration	499:511	arg1	%					530:530	10%	528:530	10%	528:530	In this study, three independent variables thought to contribute to microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%).					
36235081	2	58	dep	concentration	499:511	arg1	%					517:517	2.5%	514:517	2.5%	514:517	In this study, three independent variables thought to contribute to microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%).					
36235081	2	58	dep	concentration	499:511	arg1	%					521:521	5%	520:521	5%	520:521	In this study, three independent variables thought to contribute to microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%).					
36235081	7	59	theme	microwave	1359:1367	arg1	power					1369:1373	450 W microwave power	1353:1373	450 W microwave power	1353:1373	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	9	60	theme	pod	1703:1705	arg1	husk					1707:1710	cocoa pod husk	1697:1710	cocoa pod husk using this method	1697:1728	Pectin extracted from cocoa pod husk using this method has the potential to be further applied, especially in the food and pharmaceutical industries.					
36235081	4	61	theme	statistical	854:864	arg1	test					875:878	the statistical analysis test	850:878	the statistical analysis test	850:878	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	3	62	theme	variables	649:657	arg1	effect					627:632	the effect	623:632	the effect of independent variables on the parameter measured	623:683	Response surface methodology was applied using central composite design (CCD) to examine the effect of independent variables on the parameter measured.					
36235081	2	63	theme	acid	494:497	arg1	concentration					499:511	citric acid concentration	487:511	citric acid concentration (2.5%, 5%, and 10%)	487:531	In this study, three independent variables thought to contribute to microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%).					
36235081	1	64	theme	pod	119:121	arg1	husk					123:126	cocoa pod husk	113:126	cocoa pod husk	113:126	Optimization of pectin extraction from cocoa pod husk was carried out to examine independent variables that affect the quality of pectin obtained and determine the best conditions for the extraction process.					
36235081	0	65	theme	Cocoa	58:62	arg1	Pod					64:66	Cocoa Pod	58:66	Cocoa Pod	58:66	Optimization of Microwave-Assisted Pectin Extraction from Cocoa Pod Husk.					
36235081	9	66	contain	has	1730:1732	arg2	potential					1738:1746	the potential to be further applied	1734:1768	the potential to be further applied	1734:1768	Pectin extracted from cocoa pod husk using this method has the potential to be further applied, especially in the food and pharmaceutical industries.					
36235081	9	66	contain	has	1730:1732	arg1	Pectin					1675:1680	Pectin	1675:1680	Pectin extracted from cocoa pod husk using this method	1675:1728	Pectin extracted from cocoa pod husk using this method has the potential to be further applied, especially in the food and pharmaceutical industries.					
36235081	2	67	theme	citric	487:492	arg1	concentration					499:511	citric acid concentration	487:511	citric acid concentration (2.5%, 5%, and 10%)	487:531	In this study, three independent variables thought to contribute to microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%).					
36235081	3	68	theme	surface	543:549	arg1	methodology					551:561	Response surface methodology	534:561	Response surface methodology	534:561	Response surface methodology was applied using central composite design (CCD) to examine the effect of independent variables on the parameter measured.					
36235081	8	69	theme	Other	1492:1496	arg1	yield					1554:1558	a yield	1552:1558	a yield of 21.10%	1552:1568	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	69	theme	Other	1492:1496	arg1	values					1508:1513	Other parameter values	1492:1513	Other parameter values measured under these conditions	1492:1545	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	9	70	theme	cocoa	1697:1701	arg1	husk					1707:1710	cocoa pod husk	1697:1710	cocoa pod husk using this method	1697:1728	Pectin extracted from cocoa pod husk using this method has the potential to be further applied, especially in the food and pharmaceutical industries.					
36235081	8	71	theme	methoxyl	1607:1614	arg1	content					1616:1622	methoxyl content	1607:1622	methoxyl content of 6.07%	1607:1631	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	7	72	theme	W	1357:1357	arg1	power					1369:1373	450 W microwave power	1353:1373	450 W microwave power	1353:1373	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	6	73	theme	significant	1169:1179	arg1	effect					1181:1186	the most significant effect	1160:1186	the most significant effect	1160:1186	Optimization results concluded that the factors that had the most significant effect on temperature, moisture content, and ash content were extraction time and microwave power.					
36235081	4	74	theme	ash	1002:1004	arg1	content					1006:1012	ash content	1002:1012	ash content	1002:1012	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	4	75	theme	predicting	935:944	arg1	values					955:960	predicting response values	935:960	predicting response values	935:960	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	8	76	theme	parameter	1498:1506	arg1	yield					1554:1558	a yield	1552:1558	a yield of 21.10%	1552:1568	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	8	76	theme	parameter	1498:1506	arg1	values					1508:1513	Other parameter values	1492:1513	Other parameter values measured under these conditions	1492:1545	Other parameter values measured under these conditions were a yield of 21.10%, equivalent weight of 602.40 mg/mol, methoxyl content of 6.07%, and galacturonic acid content of 72.86%.					
36235081	3	77	theme	Response	534:541	arg1	methodology					551:561	Response surface methodology	534:561	Response surface methodology	534:561	Response surface methodology was applied using central composite design (CCD) to examine the effect of independent variables on the parameter measured.					
36235081	7	78	theme	%	1382:1382	arg1	concentration					1392:1404	10% solvent concentration	1380:1404	10% solvent concentration	1380:1404	Optimal conditions for pectin extraction were at 30 min extraction time, 450 W microwave power, and 10% solvent concentration, resulting in a temperature of 104 °C, moisture content of 6%, and ash content of 8%.					
36235081	4	79	theme	model	896:900	arg1	equations					902:910	the model equations	892:910	the model equations that were suitable for predicting response values	892:960	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	4	79	theme	model	896:900	arg1	suitable					922:929	suitable	922:929	suitable	922:929	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	4	79	theme	model	896:900	arg1	temperature					967:977	temperature	967:977	temperature	967:977	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	6	80	theme	extraction	1243:1252	arg1	time					1254:1257	extraction time	1243:1257	extraction time	1243:1257	Optimization results concluded that the factors that had the most significant effect on temperature, moisture content, and ash content were extraction time and microwave power.					
36235081	6	80	theme	extraction	1243:1252	arg1	factors					1143:1149	the factors	1139:1149	the factors that had the most significant effect on temperature, moisture content, and ash content	1139:1236	Optimization results concluded that the factors that had the most significant effect on temperature, moisture content, and ash content were extraction time and microwave power.					
36235081	4	81	theme	acid	836:839	arg1	content					841:847	galacturonic acid content	823:847	galacturonic acid content	823:847	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	4	81	theme	acid	836:839	arg1	parameters					702:711	the seven parameters	692:711	the seven parameters measured	692:720	Among the seven parameters measured, namely, yield, temperature, moisture content, ash content, equivalent weight, methoxyl content, and galacturonic acid content, the statistical analysis test showed that the model equations that were suitable for predicting response values were temperature, moisture content, and ash content.					
36235081	2	82	theme	pectin	369:374	arg1	extraction					376:385	microwave-assisted pectin extraction	350:385	microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%)	350:531	In this study, three independent variables thought to contribute to microwave-assisted pectin extraction were used, namely, extraction time (20, 25, and 30 min), microwave power (180, 300, and 450 W), and citric acid concentration (2.5%, 5%, and 10%).					
36235081	1	83	from	Optimization	74:85	arg1	husk					123:126	cocoa pod husk	113:126	cocoa pod husk	113:126	Optimization of pectin extraction from cocoa pod husk was carried out to examine independent variables that affect the quality of pectin obtained and determine the best conditions for the extraction process.					
36235081	1	84	theme	best	238:241	arg1	conditions					243:252	the best conditions	234:252	the best conditions for the extraction process	234:279	Optimization of pectin extraction from cocoa pod husk was carried out to examine independent variables that affect the quality of pectin obtained and determine the best conditions for the extraction process.					
35063479	0	0	theme	bone	101:104	arg1	engineering					113:123	bone tissue engineering	101:123	bone tissue engineering	101:123	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano apatite coatings for bone tissue engineering.					
35063479	8	1	theme	channels	1329:1336	arg1	structures					1338:1347	hollow channels structures	1322:1347	hollow channels structures	1322:1347	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	2	2	dep	scale	473:477	arg1	pores					446:450	macro pores	440:450	macro pores	440:450	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	2	2	dep	scale	473:477	arg1	channels					463:470	hollow channels	456:470	hollow channels	456:470	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	1	3	from	scale	220:224	arg1	features					192:199	hierarchically biomimetic features	166:199	hierarchically biomimetic features from nano to macro scale and high porosity	166:242	Three-dimensional grafts/scaffolds with hierarchically biomimetic features from nano to macro scale and high porosity are required for bone tissue engineering.					
35063479	1	4	theme	nano	206:209	arg1	scale					220:224	nano to macro scale	206:224	nano to macro scale	206:224	Three-dimensional grafts/scaffolds with hierarchically biomimetic features from nano to macro scale and high porosity are required for bone tissue engineering.					
35063479	3	5	theme	scaffolds	701:709	arg1	surfaces					672:679	both inner and outer surfaces	651:679	both inner and outer surfaces of the hollow fiber scaffolds	651:709	Nano apatites were coated on both inner and outer surfaces of the hollow fiber scaffolds, homogeneously.					
35063479	8	6	theme	apatite	1301:1307	arg1	coatings					1309:1316	homogeneous nano apatite coatings	1284:1316	homogeneous nano apatite coatings	1284:1316	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	5	7	dep	in	837:838	arg1	vitro					840:844	vitro	840:844	vitro	840:844	The in vitro cell experiments showed that the scaffolds with good biocompatibility could support cells adhesion and proliferation.					
35063479	2	8	theme	co-axial	551:558	arg1	printing					563:570	co-axial 3D printing	551:570	co-axial 3D printing	551:570	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	7	9	theme	bone	1165:1168	arg1	formation					1170:1178	bone formation	1165:1178	bone formation	1165:1178	The in vivo study indicated that the hollow fiber scaffolds with biomimetic nano apatite coatings showed the capability to enhance bone formation after 12 weeks of implantation.					
35063479	4	10	from	scaffolds	809:817	arg1	release					792:798	sustained release	782:798	sustained release from the scaffolds over 28 days	782:830	Proteins were directly loaded in the bioinks achieving sustained release from the scaffolds over 28 days.					
35063479	0	11	theme	tissue	106:111	arg1	engineering					113:123	bone tissue engineering	101:123	bone tissue engineering	101:123	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano apatite coatings for bone tissue engineering.					
35063479	4	12	theme	sustained	782:790	arg1	release					792:798	sustained release	782:798	sustained release from the scaffolds over 28 days	782:830	Proteins were directly loaded in the bioinks achieving sustained release from the scaffolds over 28 days.					
35063479	2	13	theme	composite	330:338	arg1	scaffolds					340:348	biomimetic organic/inorganic composite scaffolds	301:348	biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale	301:477	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	8	14	theme	tissue	1388:1393	arg1	engineering					1395:1405	bone tissue engineering	1383:1405	bone tissue engineering	1383:1405	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	2	15	dep	in	576:577	arg1	situ					579:582	situ	579:582	situ	579:582	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	7	16	theme	hollow	1071:1076	arg1	scaffolds					1084:1092	the hollow fiber scaffolds	1067:1092	the hollow fiber scaffolds with biomimetic nano apatite coatings	1067:1130	The in vivo study indicated that the hollow fiber scaffolds with biomimetic nano apatite coatings showed the capability to enhance bone formation after 12 weeks of implantation.					
35063479	8	17	theme	prepared	1231:1238	arg1	scaffolds					1269:1277	the prepared biomimetic organic/inorganic scaffolds	1227:1277	the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures	1227:1347	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	8	17	theme	prepared	1231:1238	arg1	candidates					1368:1377	potential candidates	1358:1377	potential candidates for bone tissue engineering	1358:1405	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	2	18	theme	organic/inorganic	312:328	arg1	scaffolds					340:348	biomimetic organic/inorganic composite scaffolds	301:348	biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale	301:477	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	5	19	theme	good	894:897	arg1	biocompatibility					899:914	good biocompatibility	894:914	good biocompatibility	894:914	The in vitro cell experiments showed that the scaffolds with good biocompatibility could support cells adhesion and proliferation.					
35063479	1	20	theme	macro	214:218	arg1	scale					220:224	nano to macro scale	206:224	nano to macro scale	206:224	Three-dimensional grafts/scaffolds with hierarchically biomimetic features from nano to macro scale and high porosity are required for bone tissue engineering.					
35063479	2	21	theme	alginate/GelMA	524:537	arg1	bioinks					539:545	highly concentrated alginate/GelMA bioinks	504:545	highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization	504:597	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	2	22	theme	hollow	456:461	arg1	channels					463:470	hollow channels	456:470	hollow channels	456:470	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	7	23	with	scaffolds	1084:1092	arg1	coatings					1123:1130	biomimetic nano apatite coatings	1099:1130	biomimetic nano apatite coatings	1099:1130	The in vivo study indicated that the hollow fiber scaffolds with biomimetic nano apatite coatings showed the capability to enhance bone formation after 12 weeks of implantation.					
35063479	0	24	theme	printed	3:9	arg1	scaffolds					60:68	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds	0:68	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano	0:78	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano apatite coatings for bone tissue engineering.					
35063479	1	25	with	grafts/scaffolds	144:159	arg1	features					192:199	hierarchically biomimetic features	166:199	hierarchically biomimetic features from nano to macro scale and high porosity	166:242	Three-dimensional grafts/scaffolds with hierarchically biomimetic features from nano to macro scale and high porosity are required for bone tissue engineering.					
35063479	5	26	dep	cells	930:934	arg1	cells					930:934	cells adhesion and proliferation	930:961	cells adhesion and proliferation	930:961	The in vitro cell experiments showed that the scaffolds with good biocompatibility could support cells adhesion and proliferation.					
35063479	5	26	dep	cells	930:934	arg1	proliferation					949:961	proliferation	949:961	proliferation	949:961	The in vitro cell experiments showed that the scaffolds with good biocompatibility could support cells adhesion and proliferation.					
35063479	5	26	dep	cells	930:934	arg1	adhesion					936:943	adhesion	936:943	adhesion	936:943	The in vitro cell experiments showed that the scaffolds with good biocompatibility could support cells adhesion and proliferation.					
35063479	2	27	theme	apatite	412:418	arg1	coatings					420:427	nano apatite coatings	407:427	nano apatite coatings	407:427	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	2	27	theme	apatite	412:418	arg1	nano					401:404	nano	401:404	nano (nano apatite coatings) to macro (macro pores and hollow channels) scale	401:477	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	0	28	theme	3D	0:1	arg1	scaffolds					60:68	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds	0:68	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano	0:78	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano apatite coatings for bone tissue engineering.					
35063479	7	29	theme	in	1038:1039	arg1	study					1046:1050	The in vivo study	1034:1050	The in vivo study	1034:1050	The in vivo study indicated that the hollow fiber scaffolds with biomimetic nano apatite coatings showed the capability to enhance bone formation after 12 weeks of implantation.					
35063479	8	30	theme	hollow	1322:1327	arg1	structures					1338:1347	hollow channels structures	1322:1347	hollow channels structures	1322:1347	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	8	31	theme	homogeneous	1284:1294	arg1	coatings					1309:1316	homogeneous nano apatite coatings	1284:1316	homogeneous nano apatite coatings	1284:1316	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	2	32	theme	nano	407:410	arg1	coatings					420:427	nano apatite coatings	407:427	nano apatite coatings	407:427	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	2	32	theme	nano	407:410	arg1	nano					401:404	nano	401:404	nano (nano apatite coatings) to macro (macro pores and hollow channels) scale	401:477	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	0	33	theme	alginate/GelMA	24:37	arg1	scaffolds					60:68	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds	0:68	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano	0:78	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano apatite coatings for bone tissue engineering.					
35063479	5	34	theme	in	837:838	arg1	experiments					851:861	The in vitro cell experiments	833:861	The in vitro cell experiments	833:861	The in vitro cell experiments showed that the scaffolds with good biocompatibility could support cells adhesion and proliferation.					
35063479	2	35	theme	in	576:577	arg1	mineralization					584:597	in situ mineralization	576:597	in situ mineralization	576:597	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	2	36	from	nano	401:404	arg1	porosity					360:367	high porosity	355:367	high porosity (78.7 ± 3.2%)	355:381	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	2	36	from	nano	401:404	arg1	%					380:380	78.7 ± 3.2%	370:380	78.7 ± 3.2%	370:380	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	2	36	from	nano	401:404	arg1	features					387:394	features	387:394	features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale	387:477	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	2	37	theme	high	355:358	arg1	porosity					360:367	high porosity	355:367	high porosity (78.7 ± 3.2%)	355:381	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	2	37	theme	high	355:358	arg1	%					380:380	78.7 ± 3.2%	370:380	78.7 ± 3.2%	370:380	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	7	38	dep	in	1038:1039	arg1	vivo					1041:1044	vivo	1041:1044	vivo	1041:1044	The in vivo study indicated that the hollow fiber scaffolds with biomimetic nano apatite coatings showed the capability to enhance bone formation after 12 weeks of implantation.					
35063479	2	39	theme	macro	440:444	arg1	pores					446:450	macro pores	440:450	macro pores	440:450	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	0	40	theme	concentrated	11:22	arg1	scaffolds					60:68	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds	0:68	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano	0:78	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano apatite coatings for bone tissue engineering.					
35063479	6	41	theme	osteogenic	1011:1020	arg1	capability					1022:1031	remarkable osteogenic capability	1000:1031	remarkable osteogenic capability	1000:1031	The nano apatite coatings presented remarkable osteogenic capability.					
35063479	3	42	theme	outer	666:670	arg1	surfaces					672:679	both inner and outer surfaces	651:679	both inner and outer surfaces of the hollow fiber scaffolds	651:709	Nano apatites were coated on both inner and outer surfaces of the hollow fiber scaffolds, homogeneously.					
35063479	1	43	theme	Three-dimensional	126:142	arg1	grafts/scaffolds					144:159	Three-dimensional grafts/scaffolds	126:159	Three-dimensional grafts/scaffolds with hierarchically biomimetic features from nano to macro scale and high porosity	126:242	Three-dimensional grafts/scaffolds with hierarchically biomimetic features from nano to macro scale and high porosity are required for bone tissue engineering.					
35063479	8	44	theme	nano	1296:1299	arg1	coatings					1309:1316	homogeneous nano apatite coatings	1284:1316	homogeneous nano apatite coatings	1284:1316	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	6	45	theme	remarkable	1000:1009	arg1	capability					1022:1031	remarkable osteogenic capability	1000:1031	remarkable osteogenic capability	1000:1031	The nano apatite coatings presented remarkable osteogenic capability.					
35063479	7	46	theme	nano	1110:1113	arg1	coatings					1123:1130	biomimetic nano apatite coatings	1099:1130	biomimetic nano apatite coatings	1099:1130	The in vivo study indicated that the hollow fiber scaffolds with biomimetic nano apatite coatings showed the capability to enhance bone formation after 12 weeks of implantation.					
35063479	3	47	theme	Nano	622:625	arg1	apatites					627:634	Nano apatites	622:634	Nano apatites	622:634	Nano apatites were coated on both inner and outer surfaces of the hollow fiber scaffolds, homogeneously.					
35063479	1	48	theme	high	230:233	arg1	porosity					235:242	high porosity	230:242	high porosity	230:242	Three-dimensional grafts/scaffolds with hierarchically biomimetic features from nano to macro scale and high porosity are required for bone tissue engineering.					
35063479	2	49	theme	macro	433:437	arg1	scale					473:477	macro (macro pores and hollow channels) scale	433:477	macro (macro pores and hollow channels) scale	433:477	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	0	50	theme	hollow-fibers-packed	39:58	arg1	scaffolds					60:68	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds	0:68	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano	0:78	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano apatite coatings for bone tissue engineering.					
35063479	2	51	theme	3D	560:561	arg1	printing					563:570	co-axial 3D printing	551:570	co-axial 3D printing	551:570	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	3	52	theme	inner	656:660	arg1	surfaces					672:679	both inner and outer surfaces	651:679	both inner and outer surfaces of the hollow fiber scaffolds	651:709	Nano apatites were coated on both inner and outer surfaces of the hollow fiber scaffolds, homogeneously.					
35063479	8	53	theme	potential	1358:1366	arg1	scaffolds					1269:1277	the prepared biomimetic organic/inorganic scaffolds	1227:1277	the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures	1227:1347	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	8	53	theme	potential	1358:1366	arg1	candidates					1368:1377	potential candidates	1358:1377	potential candidates for bone tissue engineering	1358:1405	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	8	54	theme	bone	1383:1386	arg1	engineering					1395:1405	bone tissue engineering	1383:1405	bone tissue engineering	1383:1405	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	2	55	theme	mild	605:608	arg1	conditions					610:619	mild conditions	605:619	mild conditions	605:619	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	7	56	theme	fiber	1078:1082	arg1	scaffolds					1084:1092	the hollow fiber scaffolds	1067:1092	the hollow fiber scaffolds with biomimetic nano apatite coatings	1067:1130	The in vivo study indicated that the hollow fiber scaffolds with biomimetic nano apatite coatings showed the capability to enhance bone formation after 12 weeks of implantation.					
35063479	7	57	theme	apatite	1115:1121	arg1	coatings					1123:1130	biomimetic nano apatite coatings	1099:1130	biomimetic nano apatite coatings	1099:1130	The in vivo study indicated that the hollow fiber scaffolds with biomimetic nano apatite coatings showed the capability to enhance bone formation after 12 weeks of implantation.					
35063479	7	58	theme	implantation	1198:1209	arg1	12 weeks					1186:1193	12 weeks	1186:1193	12 weeks of implantation	1186:1209	The in vivo study indicated that the hollow fiber scaffolds with biomimetic nano apatite coatings showed the capability to enhance bone formation after 12 weeks of implantation.					
35063479	6	59	theme	apatite	973:979	arg1	coatings					981:988	The nano apatite coatings	964:988	The nano apatite coatings	964:988	The nano apatite coatings presented remarkable osteogenic capability.					
35063479	7	60	theme	biomimetic	1099:1108	arg1	coatings					1123:1130	biomimetic nano apatite coatings	1099:1130	biomimetic nano apatite coatings	1099:1130	The in vivo study indicated that the hollow fiber scaffolds with biomimetic nano apatite coatings showed the capability to enhance bone formation after 12 weeks of implantation.					
35063479	3	61	theme	hollow	688:693	arg1	scaffolds					701:709	the hollow fiber scaffolds	684:709	the hollow fiber scaffolds	684:709	Nano apatites were coated on both inner and outer surfaces of the hollow fiber scaffolds, homogeneously.					
35063479	1	62	dep	scale	220:224	arg1	to					211:212	to	211:212	to	211:212	Three-dimensional grafts/scaffolds with hierarchically biomimetic features from nano to macro scale and high porosity are required for bone tissue engineering.					
35063479	1	63	from	porosity	235:242	arg1	features					192:199	hierarchically biomimetic features	166:199	hierarchically biomimetic features from nano to macro scale and high porosity	166:242	Three-dimensional grafts/scaffolds with hierarchically biomimetic features from nano to macro scale and high porosity are required for bone tissue engineering.					
35063479	6	64	theme	nano	968:971	arg1	coatings					981:988	The nano apatite coatings	964:988	The nano apatite coatings	964:988	The nano apatite coatings presented remarkable osteogenic capability.					
35063479	5	65	with	scaffolds	879:887	arg1	biocompatibility					899:914	good biocompatibility	894:914	good biocompatibility	894:914	The in vitro cell experiments showed that the scaffolds with good biocompatibility could support cells adhesion and proliferation.					
35063479	3	66	theme	fiber	695:699	arg1	scaffolds					701:709	the hollow fiber scaffolds	684:709	the hollow fiber scaffolds	684:709	Nano apatites were coated on both inner and outer surfaces of the hollow fiber scaffolds, homogeneously.					
35063479	1	67	theme	bone	261:264	arg1	engineering					273:283	bone tissue engineering	261:283	bone tissue engineering	261:283	Three-dimensional grafts/scaffolds with hierarchically biomimetic features from nano to macro scale and high porosity are required for bone tissue engineering.					
35063479	2	68	theme	biomimetic	301:310	arg1	scaffolds					340:348	biomimetic organic/inorganic composite scaffolds	301:348	biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale	301:477	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	8	69	theme	biomimetic	1240:1249	arg1	scaffolds					1269:1277	the prepared biomimetic organic/inorganic scaffolds	1227:1277	the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures	1227:1347	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	8	69	theme	biomimetic	1240:1249	arg1	candidates					1368:1377	potential candidates	1358:1377	potential candidates for bone tissue engineering	1358:1405	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	8	70	from	candidates	1368:1377	arg1	conclusion					1215:1224	conclusion	1215:1224	conclusion	1215:1224	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	1	71	theme	biomimetic	181:190	arg1	features					192:199	hierarchically biomimetic features	166:199	hierarchically biomimetic features from nano to macro scale and high porosity	166:242	Three-dimensional grafts/scaffolds with hierarchically biomimetic features from nano to macro scale and high porosity are required for bone tissue engineering.					
35063479	1	72	theme	tissue	266:271	arg1	engineering					273:283	bone tissue engineering	261:283	bone tissue engineering	261:283	Three-dimensional grafts/scaffolds with hierarchically biomimetic features from nano to macro scale and high porosity are required for bone tissue engineering.					
35063479	2	73	theme	concentrated	511:522	arg1	bioinks					539:545	highly concentrated alginate/GelMA bioinks	504:545	highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization	504:597	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	8	74	with	scaffolds	1269:1277	arg1	structures					1338:1347	hollow channels structures	1322:1347	hollow channels structures	1322:1347	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	8	74	with	scaffolds	1269:1277	arg1	coatings					1309:1316	homogeneous nano apatite coatings	1284:1316	homogeneous nano apatite coatings	1284:1316	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	2	75	with	scaffolds	340:348	arg1	porosity					360:367	high porosity	355:367	high porosity (78.7 ± 3.2%)	355:381	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	2	75	with	scaffolds	340:348	arg1	%					380:380	78.7 ± 3.2%	370:380	78.7 ± 3.2%	370:380	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	2	75	with	scaffolds	340:348	arg1	features					387:394	features	387:394	features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale	387:477	In this study, biomimetic organic/inorganic composite scaffolds with high porosity (78.7 ± 3.2%) and features from nano (nano apatite coatings) to macro (macro pores and hollow channels) scale were fabricated based on highly concentrated alginate/GelMA bioinks via co-axial 3D printing and in situ mineralization under mild conditions.					
35063479	5	76	theme	cell	846:849	arg1	experiments					851:861	The in vitro cell experiments	833:861	The in vitro cell experiments	833:861	The in vitro cell experiments showed that the scaffolds with good biocompatibility could support cells adhesion and proliferation.					
35063479	0	77	with	scaffolds	60:68	arg1	nano					75:78	nano	75:78	nano	75:78	3D printed concentrated alginate/GelMA hollow-fibers-packed scaffolds with nano apatite coatings for bone tissue engineering.					
35063479	8	78	theme	organic/inorganic	1251:1267	arg1	scaffolds					1269:1277	the prepared biomimetic organic/inorganic scaffolds	1227:1277	the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures	1227:1347	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35063479	8	78	theme	organic/inorganic	1251:1267	arg1	candidates					1368:1377	potential candidates	1358:1377	potential candidates for bone tissue engineering	1358:1405	In conclusion, the prepared biomimetic organic/inorganic scaffolds with homogeneous nano apatite coatings and hollow channels structures might be potential candidates for bone tissue engineering.					
35429711	0	0	theme	efficient	92:100	arg1	removal					102:108	efficient removal	92:108	efficient removal of tetracycline	92:124	Floatable cellulose acetate beads embedded with flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline.					
35429711	1	1	from	systems	197:203	arg1	issue					222:226	an imperative issue	208:226	an imperative issue from both human-health and ecological perspectives	208:277	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	1	1	from	systems	197:203	arg1	Removal					127:133	Removal	127:133	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems	127:203	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	5	2	theme	290.69 mg/g	919:929	arg1	capacity					907:914	a maximal adsorption capacity	886:914	a maximal adsorption capacity of 290.69 mg/g at 25 °C	886:938	Furthermore, the adsorption data fit Temkin > Langmuir > Freundlich with a maximal adsorption capacity of 290.69 mg/g at 25 °C, while the adsorption kinetics were well-described by pseudo-second order kinetics.					
35429711	5	3	theme	pseudo-second	994:1006	arg1	kinetics					1014:1021	pseudo-second order kinetics	994:1021	pseudo-second order kinetics	994:1021	Furthermore, the adsorption data fit Temkin > Langmuir > Freundlich with a maximal adsorption capacity of 290.69 mg/g at 25 °C, while the adsorption kinetics were well-described by pseudo-second order kinetics.					
35429711	5	4	with	Temkin > Langmuir > Freundlich	850:879	arg1	capacity					907:914	a maximal adsorption capacity	886:914	a maximal adsorption capacity of 290.69 mg/g at 25 °C	886:938	Furthermore, the adsorption data fit Temkin > Langmuir > Freundlich with a maximal adsorption capacity of 290.69 mg/g at 25 °C, while the adsorption kinetics were well-described by pseudo-second order kinetics.					
35429711	7	5	theme	better	1178:1183	arg1	property					1196:1203	better adsorption property	1178:1203	better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%	1178:1265	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	7	6	theme	floated	1134:1140	arg1	beads					1162:1166	the floated UiO-66/ZIF-8/PDA@CA beads	1130:1166	the floated UiO-66/ZIF-8/PDA@CA beads	1130:1166	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	4	7	theme	surface	700:706	arg1	area					708:711	large specific surface area	685:711	large specific surface area	685:711	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	4	8	with	ease	796:799	arg1	time					778:781	a relatively short equilibrium time	747:781	a relatively short equilibrium time (60 min)	747:790	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	4	8	with	ease	796:799	arg1	60 min					784:789	60 min	784:789	60 min	784:789	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	6	9	theme	adsorption	1069:1078	arg1	endothermic					1092:1102	endothermic	1092:1102	endothermic	1092:1102	The thermodynamics studies refereed that the adsorption process was endothermic and spontaneous.					
35429711	6	9	theme	adsorption	1069:1078	arg1	process					1080:1086	the adsorption process	1065:1086	the adsorption process	1065:1086	The thermodynamics studies refereed that the adsorption process was endothermic and spontaneous.					
35429711	3	10	theme	composite	497:505	arg1	beads					507:511	The formulated composite beads	482:511	The formulated composite beads	482:511	The formulated composite beads were characterized by FTIR, XRD, SEM, BET, Zeta potential and XPS analysis tools.					
35429711	7	11	theme	CA	1159:1160	arg1	beads					1162:1166	the floated UiO-66/ZIF-8/PDA@CA beads	1130:1166	the floated UiO-66/ZIF-8/PDA@CA beads	1130:1166	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	2	12	theme	@	337:337	arg1	CA					384:385	UiO-66/ZIF-8/PDA@CA	367:385	UiO-66/ZIF-8/PDA@CA	367:385	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	2	12	theme	@	337:337	arg1	composite					356:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite	288:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA)	288:386	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	0	13	with	embedded	34:41	arg1	MOF/PDA					80:86	flower-like zwitterionic binary MOF/PDA	48:86	flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline	48:124	Floatable cellulose acetate beads embedded with flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline.					
35429711	7	14	from	bodies	1377:1382	arg1	removal					1343:1349	efficient removal	1333:1349	efficient removal of antibiotics from water bodies	1333:1382	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	4	15	with	area	708:711	arg1	time					778:781	a relatively short equilibrium time	747:781	a relatively short equilibrium time (60 min)	747:790	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	4	15	with	area	708:711	arg1	60 min					784:789	60 min	784:789	60 min	784:789	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	4	16	theme	equilibrium	766:776	arg1	time					778:781	a relatively short equilibrium time	747:781	a relatively short equilibrium time (60 min)	747:790	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	4	16	theme	equilibrium	766:776	arg1	60 min					784:789	60 min	784:789	60 min	784:789	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	2	17	theme	MOF/polydopamine	321:336	arg1	CA					384:385	UiO-66/ZIF-8/PDA@CA	367:385	UiO-66/ZIF-8/PDA@CA	367:385	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	2	17	theme	MOF/polydopamine	321:336	arg1	composite					356:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite	288:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA)	288:386	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	2	18	theme	UiO-66/ZIF-8/PDA	367:382	arg1	CA					384:385	UiO-66/ZIF-8/PDA@CA	367:385	UiO-66/ZIF-8/PDA@CA	367:385	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	2	18	theme	UiO-66/ZIF-8/PDA	367:382	arg1	composite					356:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite	288:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA)	288:386	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	7	19	theme	maximal	1235:1241	arg1	removal					1243:1249	a maximal removal	1233:1249	a maximal removal	1233:1249	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	7	20	theme	UiO-66/ZIF-8/PDA	1142:1157	arg1	beads					1162:1166	the floated UiO-66/ZIF-8/PDA@CA beads	1130:1166	the floated UiO-66/ZIF-8/PDA@CA beads	1130:1166	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	3	21	theme	formulated	486:495	arg1	beads					507:511	The formulated composite beads	482:511	The formulated composite beads	482:511	The formulated composite beads were characterized by FTIR, XRD, SEM, BET, Zeta potential and XPS analysis tools.					
35429711	2	22	theme	binary	314:319	arg1	CA					384:385	UiO-66/ZIF-8/PDA@CA	367:385	UiO-66/ZIF-8/PDA@CA	367:385	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	2	22	theme	binary	314:319	arg1	composite					356:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite	288:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA)	288:386	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	7	23	theme	antibiotics	1354:1364	arg1	removal					1343:1349	efficient removal	1333:1349	efficient removal of antibiotics from water bodies	1333:1382	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	1	24	theme	aquatic	189:195	arg1	systems					197:203	aquatic systems	189:203	aquatic systems	189:203	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	0	25	theme	tetracycline	113:124	arg1	removal					102:108	efficient removal	92:108	efficient removal of tetracycline	92:124	Floatable cellulose acetate beads embedded with flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline.					
35429711	7	26	theme	@	1158:1158	arg1	beads					1162:1166	the floated UiO-66/ZIF-8/PDA@CA beads	1130:1166	the floated UiO-66/ZIF-8/PDA@CA beads	1130:1166	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	7	27	theme	adsorption	1185:1194	arg1	property					1196:1203	better adsorption property	1178:1203	better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%	1178:1265	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	2	28	theme	UiO-66/ZIF-8	301:312	arg1	CA					384:385	UiO-66/ZIF-8/PDA@CA	367:385	UiO-66/ZIF-8/PDA@CA	367:385	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	2	28	theme	UiO-66/ZIF-8	301:312	arg1	composite					356:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite	288:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA)	288:386	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	2	29	theme	floated	419:425	arg1	beads					427:431	floated beads	419:431	floated beads	419:431	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	0	30	theme	cellulose	10:18	arg1	beads					28:32	Floatable cellulose acetate beads	0:32	Floatable cellulose acetate beads	0:32	Floatable cellulose acetate beads embedded with flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline.					
35429711	5	31	theme	adsorption	951:960	arg1	well-described					976:989	well-described	976:989	well-described	976:989	Furthermore, the adsorption data fit Temkin > Langmuir > Freundlich with a maximal adsorption capacity of 290.69 mg/g at 25 °C, while the adsorption kinetics were well-described by pseudo-second order kinetics.					
35429711	5	31	theme	adsorption	951:960	arg1	kinetics					962:969	the adsorption kinetics	947:969	the adsorption kinetics	947:969	Furthermore, the adsorption data fit Temkin > Langmuir > Freundlich with a maximal adsorption capacity of 290.69 mg/g at 25 °C, while the adsorption kinetics were well-described by pseudo-second order kinetics.					
35429711	5	32	theme	maximal	888:894	arg1	capacity					907:914	a maximal adsorption capacity	886:914	a maximal adsorption capacity of 290.69 mg/g at 25 °C	886:938	Furthermore, the adsorption data fit Temkin > Langmuir > Freundlich with a maximal adsorption capacity of 290.69 mg/g at 25 °C, while the adsorption kinetics were well-described by pseudo-second order kinetics.					
35429711	0	33	theme	Floatable	0:8	arg1	beads					28:32	Floatable cellulose acetate beads	0:32	Floatable cellulose acetate beads	0:32	Floatable cellulose acetate beads embedded with flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline.					
35429711	3	34	theme	Zeta	556:559	arg1	potential					561:569	Zeta potential	556:569	Zeta potential	556:569	The formulated composite beads were characterized by FTIR, XRD, SEM, BET, Zeta potential and XPS analysis tools.					
35429711	4	35	theme	adsorption	719:728	arg1	performance					730:740	good adsorption performance	714:740	good adsorption performance with a relatively short equilibrium time (60 min)	714:790	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	4	36	theme	composite	656:664	arg1	beads					666:670	the UiO-66/ZIF-8/PDA@CA composite beads	632:670	the UiO-66/ZIF-8/PDA@CA composite beads	632:670	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	7	37	with	cycles	1221:1226	arg1	removal					1243:1249	a maximal removal	1233:1249	a maximal removal	1233:1249	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	5	38	theme	adsorption	896:905	arg1	capacity					907:914	a maximal adsorption capacity	886:914	a maximal adsorption capacity of 290.69 mg/g at 25 °C	886:938	Furthermore, the adsorption data fit Temkin > Langmuir > Freundlich with a maximal adsorption capacity of 290.69 mg/g at 25 °C, while the adsorption kinetics were well-described by pseudo-second order kinetics.					
35429711	2	39	theme	tetracycline	463:474	arg1	removal					452:458	the adsorptive removal	437:458	the adsorptive removal of tetracycline (TC)	437:479	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	5	40	from	25 °C	934:938	arg1	capacity					907:914	a maximal adsorption capacity	886:914	a maximal adsorption capacity of 290.69 mg/g at 25 °C	886:938	Furthermore, the adsorption data fit Temkin > Langmuir > Freundlich with a maximal adsorption capacity of 290.69 mg/g at 25 °C, while the adsorption kinetics were well-described by pseudo-second order kinetics.					
35429711	2	41	theme	acetate	348:354	arg1	CA					384:385	UiO-66/ZIF-8/PDA@CA	367:385	UiO-66/ZIF-8/PDA@CA	367:385	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	2	41	theme	acetate	348:354	arg1	composite					356:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite	288:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA)	288:386	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	1	42	theme	imperative	211:220	arg1	issue					222:226	an imperative issue	208:226	an imperative issue from both human-health and ecological perspectives	208:277	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	1	42	theme	imperative	211:220	arg1	Removal					127:133	Removal	127:133	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems	127:203	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	0	43	theme	acetate	20:26	arg1	beads					28:32	Floatable cellulose acetate beads	0:32	Floatable cellulose acetate beads	0:32	Floatable cellulose acetate beads embedded with flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline.					
35429711	4	44	theme	optimum	601:607	arg1	conditions					620:629	optimum adsorption conditions	601:629	optimum adsorption conditions	601:629	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	2	45	theme	cellulose	338:346	arg1	CA					384:385	UiO-66/ZIF-8/PDA@CA	367:385	UiO-66/ZIF-8/PDA@CA	367:385	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	2	45	theme	cellulose	338:346	arg1	composite					356:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite	288:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA)	288:386	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	2	46	theme	adsorptive	441:450	arg1	removal					452:458	the adsorptive removal	437:458	the adsorptive removal of tetracycline (TC)	437:479	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	7	47	theme	efficient	1333:1341	arg1	removal					1343:1349	efficient removal	1333:1349	efficient removal of antibiotics from water bodies	1333:1382	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	4	48	theme	@	652:652	arg1	beads					666:670	the UiO-66/ZIF-8/PDA@CA composite beads	632:670	the UiO-66/ZIF-8/PDA@CA composite beads	632:670	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	2	49	theme	beads	427:431	arg1	form					411:414	the form	407:414	the form of floated beads	407:431	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	6	50	theme	thermodynamics	1028:1041	arg1	studies					1043:1049	The thermodynamics studies	1024:1049	The thermodynamics studies	1024:1049	The thermodynamics studies refereed that the adsorption process was endothermic and spontaneous.					
35429711	7	51	theme	water	1371:1375	arg1	bodies					1377:1382	water bodies	1371:1382	water bodies	1371:1382	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	7	52	theme	reuse	1215:1219	arg1	cycles					1221:1226	eight reuse cycles	1209:1226	eight reuse cycles with a maximal removal (%) reached 67%	1209:1265	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	7	52	theme	reuse	1215:1219	arg1	%					1252:1252	%	1252:1252	%	1252:1252	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	4	53	theme	UiO-66/ZIF-8/PDA	636:651	arg1	beads					666:670	the UiO-66/ZIF-8/PDA@CA composite beads	632:670	the UiO-66/ZIF-8/PDA@CA composite beads	632:670	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	0	54	theme	zwitterionic	60:71	arg1	MOF/PDA					80:86	flower-like zwitterionic binary MOF/PDA	48:86	flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline	48:124	Floatable cellulose acetate beads embedded with flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline.					
35429711	3	55	theme	XPS	575:577	arg1	tools					588:592	XPS analysis tools	575:592	XPS analysis tools	575:592	The formulated composite beads were characterized by FTIR, XRD, SEM, BET, Zeta potential and XPS analysis tools.					
35429711	4	56	theme	good	714:717	arg1	performance					730:740	good adsorption performance	714:740	good adsorption performance with a relatively short equilibrium time (60 min)	714:790	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	0	57	theme	flower-like	48:58	arg1	MOF/PDA					80:86	flower-like zwitterionic binary MOF/PDA	48:86	flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline	48:124	Floatable cellulose acetate beads embedded with flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline.					
35429711	1	58	from	perspectives	266:277	arg1	issue					222:226	an imperative issue	208:226	an imperative issue from both human-health and ecological perspectives	208:277	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	1	58	from	perspectives	266:277	arg1	Removal					127:133	Removal	127:133	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems	127:203	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	3	59	theme	analysis	579:586	arg1	tools					588:592	XPS analysis tools	575:592	XPS analysis tools	575:592	The formulated composite beads were characterized by FTIR, XRD, SEM, BET, Zeta potential and XPS analysis tools.					
35429711	7	60	theme	promising	1283:1291	arg1	applicability					1293:1305	its promising applicability	1279:1305	its promising applicability	1279:1305	Besides, the floated UiO-66/ZIF-8/PDA@CA beads displayed better adsorption property for eight reuse cycles with a maximal removal (%) reached 67%, reflecting its promising applicability as reusable adsorbent for efficient removal of antibiotics from water bodies.					
35429711	2	61	theme	zwitterionic	288:299	arg1	CA					384:385	UiO-66/ZIF-8/PDA@CA	367:385	UiO-66/ZIF-8/PDA@CA	367:385	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	2	61	theme	zwitterionic	288:299	arg1	composite					356:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite	288:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA)	288:386	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	1	62	theme	pharmaceutical	138:151	arg1	wastes					153:158	pharmaceutical wastes	138:158	pharmaceutical wastes	138:158	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	1	62	theme	pharmaceutical	138:151	arg1	antibiotics					172:182	antibiotics	172:182	antibiotics	172:182	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	4	63	theme	short	760:764	arg1	time					778:781	a relatively short equilibrium time	747:781	a relatively short equilibrium time (60 min)	747:790	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	4	63	theme	short	760:764	arg1	60 min					784:789	60 min	784:789	60 min	784:789	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	1	64	theme	human-health	238:249	arg1	perspectives					266:277	both human-health and ecological perspectives	233:277	both human-health and ecological perspectives	233:277	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	4	65	dep	area	708:711	arg1	separation					801:810	separation	801:810	separation	801:810	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	1	66	theme	wastes	153:158	arg1	issue					222:226	an imperative issue	208:226	an imperative issue from both human-health and ecological perspectives	208:277	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	1	66	theme	wastes	153:158	arg1	Removal					127:133	Removal	127:133	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems	127:203	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	2	67	theme	@	383:383	arg1	CA					384:385	UiO-66/ZIF-8/PDA@CA	367:385	UiO-66/ZIF-8/PDA@CA	367:385	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	2	67	theme	@	383:383	arg1	composite					356:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite	288:364	zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA)	288:386	Herein, zwitterionic UiO-66/ZIF-8 binary MOF/polydopamine@cellulose acetate composite (UiO-66/ZIF-8/PDA@CA) were fabricated in the form of floated beads for the adsorptive removal of tetracycline (TC).					
35429711	5	68	theme	order	1008:1012	arg1	kinetics					1014:1021	pseudo-second order kinetics	994:1021	pseudo-second order kinetics	994:1021	Furthermore, the adsorption data fit Temkin > Langmuir > Freundlich with a maximal adsorption capacity of 290.69 mg/g at 25 °C, while the adsorption kinetics were well-described by pseudo-second order kinetics.					
35429711	5	69	theme	adsorption	830:839	arg1	data					841:844	the adsorption data	826:844	the adsorption data	826:844	Furthermore, the adsorption data fit Temkin > Langmuir > Freundlich with a maximal adsorption capacity of 290.69 mg/g at 25 °C, while the adsorption kinetics were well-described by pseudo-second order kinetics.					
35429711	4	70	theme	specific	691:698	arg1	area					708:711	large specific surface area	685:711	large specific surface area	685:711	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	4	71	theme	CA	653:654	arg1	beads					666:670	the UiO-66/ZIF-8/PDA@CA composite beads	632:670	the UiO-66/ZIF-8/PDA@CA composite beads	632:670	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	1	72	theme	ecological	255:264	arg1	perspectives					266:277	both human-health and ecological perspectives	233:277	both human-health and ecological perspectives	233:277	Removal of pharmaceutical wastes, especially antibiotics from aquatic systems is an imperative issue from both human-health and ecological perspectives.					
35429711	0	73	theme	binary	73:78	arg1	MOF/PDA					80:86	flower-like zwitterionic binary MOF/PDA	48:86	flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline	48:124	Floatable cellulose acetate beads embedded with flower-like zwitterionic binary MOF/PDA for efficient removal of tetracycline.					
35429711	4	74	with	performance	730:740	arg1	time					778:781	a relatively short equilibrium time	747:781	a relatively short equilibrium time (60 min)	747:790	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	4	74	with	performance	730:740	arg1	60 min					784:789	60 min	784:789	60 min	784:789	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	4	75	theme	large	685:689	arg1	area					708:711	large specific surface area	685:711	large specific surface area	685:711	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35429711	4	76	theme	adsorption	609:618	arg1	conditions					620:629	optimum adsorption conditions	601:629	optimum adsorption conditions	601:629	Under optimum adsorption conditions, the UiO-66/ZIF-8/PDA@CA composite beads demonstrated large specific surface area, good adsorption performance with a relatively short equilibrium time (60 min) and ease separation.					
35089712	3	0	with	solutions	658:666	arg1	masses					689:694	different molar masses	673:694	different molar masses	673:694	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	1	1	theme	cooperative	167:177	arg1	CODT					206:209	CODT	206:209	CODT	206:209	Schizophyllan, a triple helical polysaccharide, exhibits cooperative order-disorder transition (CODT) in aqueous solutions.					
35089712	1	1	theme	cooperative	167:177	arg1	transition					194:203	cooperative order-disorder transition	167:203	cooperative order-disorder transition (CODT) in aqueous solutions	167:231	Schizophyllan, a triple helical polysaccharide, exhibits cooperative order-disorder transition (CODT) in aqueous solutions.					
35089712	5	2	theme	transition	954:963	arg1	theory					965:970	linear cooperative transition theory	935:970	linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds	935:1042	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures were analyzed with linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds.					
35089712	1	3	theme	order-disorder	179:192	arg1	CODT					206:209	CODT	206:209	CODT	206:209	Schizophyllan, a triple helical polysaccharide, exhibits cooperative order-disorder transition (CODT) in aqueous solutions.					
35089712	1	3	theme	order-disorder	179:192	arg1	transition					194:203	cooperative order-disorder transition	167:203	cooperative order-disorder transition (CODT) in aqueous solutions	167:231	Schizophyllan, a triple helical polysaccharide, exhibits cooperative order-disorder transition (CODT) in aqueous solutions.					
35089712	5	4	theme	active	1027:1032	arg1	compounds					1034:1042	active compounds	1027:1042	active compounds	1027:1042	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures were analyzed with linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds.					
35089712	0	5	from	Association	0:10	arg1	Transition					61:70	the Cooperative Order-Disorder Transition	30:70	the Cooperative Order-Disorder Transition in Aqueous Solution of Schizophyllan	30:107	Association with Imidazole in the Cooperative Order-Disorder Transition in Aqueous Solution of Schizophyllan.					
35089712	6	6	from	imidazole	1086:1094	arg1	solutions					1124:1132	the solutions	1120:1132	the solutions	1120:1132	Theoretical analyses confirmed that both imidazole and imidazolium ions in the solutions competitively interact with the side chain of the triple helix.					
35089712	0	7	theme	Schizophyllan	95:107	arg1	Solution					83:90	Aqueous Solution	75:90	Aqueous Solution of Schizophyllan	75:107	Association with Imidazole in the Cooperative Order-Disorder Transition in Aqueous Solution of Schizophyllan.					
35089712	0	8	from	Transition	61:70	arg1	Solution					83:90	Aqueous Solution	75:90	Aqueous Solution of Schizophyllan	75:107	Association with Imidazole in the Cooperative Order-Disorder Transition in Aqueous Solution of Schizophyllan.					
35089712	6	9	theme	helix	1191:1195	arg1	chain					1171:1175	the side chain	1162:1175	the side chain of the triple helix	1162:1195	Theoretical analyses confirmed that both imidazole and imidazolium ions in the solutions competitively interact with the side chain of the triple helix.					
35089712	5	10	theme	solvent-stabilizing	980:998	arg1	effect					1000:1005	the solvent-stabilizing effect	976:1005	the solvent-stabilizing effect in the mixture with active compounds	976:1042	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures were analyzed with linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds.					
35089712	4	11	theme	mole	801:804	arg1	fractions					806:814	the mole fractions	797:814	the mole fractions of imidazole and imidazole/HCl	797:845	The transition temperature (Tr) and the transition enthalpy (ΔHr) significantly depended on both of the mole fractions of imidazole and imidazole/HCl.					
35089712	5	12	theme	H2O-imidazole	893:905	arg1	mixtures					907:914	H2O-imidazole mixtures	893:914	H2O-imidazole mixtures	893:914	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures were analyzed with linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds.					
35089712	4	13	theme	transition	701:710	arg1	Tr					725:726	Tr	725:726	Tr	725:726	The transition temperature (Tr) and the transition enthalpy (ΔHr) significantly depended on both of the mole fractions of imidazole and imidazole/HCl.					
35089712	4	13	theme	transition	701:710	arg1	temperature					712:722	The transition temperature	697:722	The transition temperature (Tr)	697:727	The transition temperature (Tr) and the transition enthalpy (ΔHr) significantly depended on both of the mole fractions of imidazole and imidazole/HCl.					
35089712	5	14	with	mixture	1014:1020	arg1	compounds					1034:1042	active compounds	1027:1042	active compounds	1027:1042	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures were analyzed with linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds.					
35089712	5	15	theme	linear	935:940	arg1	theory					965:970	linear cooperative transition theory	935:970	linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds	935:1042	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures were analyzed with linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds.					
35089712	2	16	theme	helix	290:294	arg1	structure					272:280	the ordered structure	260:280	the ordered structure (triple helix I) formed between the branched side chains and solvent molecules	260:359	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	2	16	theme	helix	290:294	arg1	I					296:296	triple helix I	283:296	triple helix I	283:296	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	3	17	contain	containing	496:505	arg2	HCl					507:509	HCl	507:509	HCl with different molar ratios of imidazole/HCl	507:554	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	3	17	contain	containing	496:505	arg1	mixtures					487:494	H2O-imidazole mixtures	473:494	H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl	473:554	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	2	18	theme	side	327:330	arg1	chains					332:337	the branched side chains	314:337	the branched side chains	314:337	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	2	19	theme	triple	392:397	arg1	helix					399:403	triple helix II	392:406	triple helix II	392:406	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	2	19	theme	triple	392:397	arg1	structure					381:389	the disordered structure	366:389	the disordered structure (triple helix II)	366:407	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	2	20	theme	helix	444:448	arg1	dissociation					417:428	dissociation	417:428	dissociation of the triple helix	417:448	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	3	21	with	HCl	507:509	arg1	ratios					532:537	different molar ratios	516:537	different molar ratios of imidazole/HCl	516:554	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	2	22	theme	branched	318:325	arg1	chains					332:337	the branched side chains	314:337	the branched side chains	314:337	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	2	23	theme	triple	437:442	arg1	helix					444:448	the triple helix	433:448	the triple helix	433:448	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	6	24	from	ions	1112:1115	arg1	solutions					1124:1132	the solutions	1120:1132	the solutions	1120:1132	Theoretical analyses confirmed that both imidazole and imidazolium ions in the solutions competitively interact with the side chain of the triple helix.					
35089712	4	25	theme	imidazole	819:827	arg1	fractions					806:814	the mole fractions	797:814	the mole fractions of imidazole and imidazole/HCl	797:845	The transition temperature (Tr) and the transition enthalpy (ΔHr) significantly depended on both of the mole fractions of imidazole and imidazole/HCl.					
35089712	3	26	from	behaviors	460:468	arg1	mixtures					487:494	H2O-imidazole mixtures	473:494	H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl	473:554	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	3	27	theme	adiabatic	577:585	arg1	calorimetry					587:597	adiabatic calorimetry	577:597	adiabatic calorimetry	577:597	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	3	28	from	calorimetry	625:635	arg1	solutions					658:666	two schizophyllan solutions	640:666	two schizophyllan solutions with different molar masses	640:694	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	1	29	theme	aqueous	215:221	arg1	solutions					223:231	aqueous solutions	215:231	aqueous solutions	215:231	Schizophyllan, a triple helical polysaccharide, exhibits cooperative order-disorder transition (CODT) in aqueous solutions.					
35089712	0	30	theme	Cooperative	34:44	arg1	Transition					61:70	the Cooperative Order-Disorder Transition	30:70	the Cooperative Order-Disorder Transition in Aqueous Solution of Schizophyllan	30:107	Association with Imidazole in the Cooperative Order-Disorder Transition in Aqueous Solution of Schizophyllan.					
35089712	2	31	theme	solvent	343:349	arg1	molecules					351:359	solvent molecules	343:359	solvent molecules	343:359	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	6	32	theme	imidazolium	1100:1110	arg1	ions					1112:1115	imidazolium ions	1100:1115	imidazolium ions in the solutions	1100:1132	Theoretical analyses confirmed that both imidazole and imidazolium ions in the solutions competitively interact with the side chain of the triple helix.					
35089712	5	33	theme	Tr	879:880	arg1	dependences					864:874	The composition dependences	848:874	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures	848:914	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures were analyzed with linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds.					
35089712	5	34	theme	ΔHr	886:888	arg1	dependences					864:874	The composition dependences	848:874	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures	848:914	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures were analyzed with linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds.					
35089712	6	35	theme	Theoretical	1045:1055	arg1	analyses					1057:1064	Theoretical analyses	1045:1064	Theoretical analyses	1045:1064	Theoretical analyses confirmed that both imidazole and imidazolium ions in the solutions competitively interact with the side chain of the triple helix.					
35089712	3	36	theme	different	673:681	arg1	masses					689:694	different molar masses	673:694	different molar masses	673:694	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	4	37	theme	imidazole/HCl	833:845	arg1	fractions					806:814	the mole fractions	797:814	the mole fractions of imidazole and imidazole/HCl	797:845	The transition temperature (Tr) and the transition enthalpy (ΔHr) significantly depended on both of the mole fractions of imidazole and imidazole/HCl.					
35089712	3	38	theme	CODT	455:458	arg1	behaviors					460:468	The CODT behaviors	451:468	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl	451:554	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	3	39	theme	molar	683:687	arg1	masses					689:694	different molar masses	673:694	different molar masses	673:694	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	3	40	theme	differential	603:614	arg1	calorimetry					625:635	differential scanning calorimetry	603:635	differential scanning calorimetry	603:635	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	6	41	theme	triple	1184:1189	arg1	helix					1191:1195	the triple helix	1180:1195	the triple helix	1180:1195	Theoretical analyses confirmed that both imidazole and imidazolium ions in the solutions competitively interact with the side chain of the triple helix.					
35089712	5	42	theme	cooperative	942:952	arg1	theory					965:970	linear cooperative transition theory	935:970	linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds	935:1042	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures were analyzed with linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds.					
35089712	0	43	theme	Order-Disorder	46:59	arg1	Transition					61:70	the Cooperative Order-Disorder Transition	30:70	the Cooperative Order-Disorder Transition in Aqueous Solution of Schizophyllan	30:107	Association with Imidazole in the Cooperative Order-Disorder Transition in Aqueous Solution of Schizophyllan.					
35089712	3	44	theme	scanning	616:623	arg1	calorimetry					625:635	differential scanning calorimetry	603:635	differential scanning calorimetry	603:635	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	3	45	theme	different	516:524	arg1	ratios					532:537	different molar ratios	516:537	different molar ratios of imidazole/HCl	516:554	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	1	46	theme	triple	127:132	arg1	polysaccharide					142:155	a triple helical polysaccharide	125:155	a triple helical polysaccharide	125:155	Schizophyllan, a triple helical polysaccharide, exhibits cooperative order-disorder transition (CODT) in aqueous solutions.					
35089712	1	46	theme	triple	127:132	arg1	Schizophyllan					110:122	Schizophyllan	110:122	Schizophyllan	110:122	Schizophyllan, a triple helical polysaccharide, exhibits cooperative order-disorder transition (CODT) in aqueous solutions.					
35089712	5	47	from	effect	1000:1005	arg1	mixture					1014:1020	the mixture	1010:1020	the mixture with active compounds	1010:1042	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures were analyzed with linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds.					
35089712	3	48	theme	schizophyllan	644:656	arg1	solutions					658:666	two schizophyllan solutions	640:666	two schizophyllan solutions with different molar masses	640:694	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	2	49	theme	triple	283:288	arg1	structure					272:280	the ordered structure	260:280	the ordered structure (triple helix I) formed between the branched side chains and solvent molecules	260:359	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	2	49	theme	triple	283:288	arg1	I					296:296	triple helix I	283:296	triple helix I	283:296	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	0	50	with	Association	0:10	arg1	Imidazole					17:25	Imidazole	17:25	Imidazole	17:25	Association with Imidazole in the Cooperative Order-Disorder Transition in Aqueous Solution of Schizophyllan.					
35089712	1	51	theme	helical	134:140	arg1	polysaccharide					142:155	a triple helical polysaccharide	125:155	a triple helical polysaccharide	125:155	Schizophyllan, a triple helical polysaccharide, exhibits cooperative order-disorder transition (CODT) in aqueous solutions.					
35089712	1	51	theme	helical	134:140	arg1	Schizophyllan					110:122	Schizophyllan	110:122	Schizophyllan	110:122	Schizophyllan, a triple helical polysaccharide, exhibits cooperative order-disorder transition (CODT) in aqueous solutions.					
35089712	5	52	from	dependences	864:874	arg1	mixtures					907:914	H2O-imidazole mixtures	893:914	H2O-imidazole mixtures	893:914	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures were analyzed with linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds.					
35089712	3	53	theme	imidazole/HCl	542:554	arg1	ratios					532:537	different molar ratios	516:537	different molar ratios of imidazole/HCl	516:554	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	5	54	theme	composition	852:862	arg1	dependences					864:874	The composition dependences	848:874	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures	848:914	The composition dependences of Tr and ΔHr in H2O-imidazole mixtures were analyzed with linear cooperative transition theory for the solvent-stabilizing effect in the mixture with active compounds.					
35089712	6	55	theme	side	1166:1169	arg1	chain					1171:1175	the side chain	1162:1175	the side chain of the triple helix	1162:1195	Theoretical analyses confirmed that both imidazole and imidazolium ions in the solutions competitively interact with the side chain of the triple helix.					
35089712	3	56	from	calorimetry	587:597	arg1	solutions					658:666	two schizophyllan solutions	640:666	two schizophyllan solutions with different molar masses	640:694	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	3	57	theme	H2O-imidazole	473:485	arg1	mixtures					487:494	H2O-imidazole mixtures	473:494	H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl	473:554	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	0	58	theme	Aqueous	75:81	arg1	Solution					83:90	Aqueous Solution	75:90	Aqueous Solution of Schizophyllan	75:107	Association with Imidazole in the Cooperative Order-Disorder Transition in Aqueous Solution of Schizophyllan.					
35089712	3	59	theme	molar	526:530	arg1	ratios					532:537	different molar ratios	516:537	different molar ratios of imidazole/HCl	516:554	The CODT behaviors in H2O-imidazole mixtures containing HCl with different molar ratios of imidazole/HCl were investigated by adiabatic calorimetry and differential scanning calorimetry on two schizophyllan solutions with different molar masses.					
35089712	2	60	theme	ordered	264:270	arg1	structure					272:280	the ordered structure	260:280	the ordered structure (triple helix I) formed between the branched side chains and solvent molecules	260:359	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	2	60	theme	ordered	264:270	arg1	I					296:296	triple helix I	283:296	triple helix I	283:296	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	1	61	from	transition	194:203	arg1	solutions					223:231	aqueous solutions	215:231	aqueous solutions	215:231	Schizophyllan, a triple helical polysaccharide, exhibits cooperative order-disorder transition (CODT) in aqueous solutions.					
35089712	2	62	theme	disordered	370:379	arg1	helix					399:403	triple helix II	392:406	triple helix II	392:406	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	2	62	theme	disordered	370:379	arg1	structure					381:389	the disordered structure	366:389	the disordered structure (triple helix II)	366:407	The transition transforms the ordered structure (triple helix I) formed between the branched side chains and solvent molecules into the disordered structure (triple helix II) without dissociation of the triple helix.					
35089712	4	63	theme	transition	737:746	arg1	enthalpy					748:755	the transition enthalpy	733:755	the transition enthalpy (ΔHr)	733:761	The transition temperature (Tr) and the transition enthalpy (ΔHr) significantly depended on both of the mole fractions of imidazole and imidazole/HCl.					
35089712	4	63	theme	transition	737:746	arg1	ΔHr					758:760	ΔHr	758:760	ΔHr	758:760	The transition temperature (Tr) and the transition enthalpy (ΔHr) significantly depended on both of the mole fractions of imidazole and imidazole/HCl.					
35510868	6	0	theme	consumption	934:944	arg1	modeling					946:953	energy consumption modeling	927:953	energy consumption modeling of using such aerogel coolers in buildings in China	927:1005	Furthermore, energy consumption modeling of using such aerogel coolers in buildings in China shows 35.4% reduction of cooling energy.					
35510868	4	1	theme	moist	752:756	arg1	surroundings					778:789	hot, moist, and fickle extreme surroundings	747:789	hot, moist, and fickle extreme surroundings	747:789	Such aerogel coolers can achieve a subambient temperature drop of 9.2 °C under direct sunlight and promisingly reached the reduction of ∼7.4 °C even in hot, moist, and fickle extreme surroundings.					
35510868	2	2	theme	hot	380:382	arg1	regions					391:397	hot, humid regions	380:397	hot, humid regions	380:397	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	4	3	theme	aerogel	600:606	arg1	coolers					608:614	Such aerogel coolers	595:614	Such aerogel coolers	595:614	Such aerogel coolers can achieve a subambient temperature drop of 9.2 °C under direct sunlight and promisingly reached the reduction of ∼7.4 °C even in hot, moist, and fickle extreme surroundings.					
35510868	6	4	theme	energy	927:932	arg1	modeling					946:953	energy consumption modeling	927:953	energy consumption modeling of using such aerogel coolers in buildings in China	927:1005	Furthermore, energy consumption modeling of using such aerogel coolers in buildings in China shows 35.4% reduction of cooling energy.					
35510868	7	5	theme	consumption	1150:1160	arg1	savings					1162:1168	energy consumption savings	1143:1168	energy consumption savings	1143:1168	This work can pave the way toward designing high-performance, thermal-regulating materials for energy consumption savings.					
35510868	2	6	from	challenge	355:363	arg1	regions					391:397	hot, humid regions	380:397	hot, humid regions	380:397	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	1	7	theme	significant	177:187	arg1	impact					189:194	a significant impact	175:194	a significant impact on overall energy consumption	175:224	A passive cooling strategy without any electricity input has shown a significant impact on overall energy consumption globally.					
35510868	4	8	theme	Such	595:598	arg1	coolers					608:614	Such aerogel coolers	595:614	Such aerogel coolers	595:614	Such aerogel coolers can achieve a subambient temperature drop of 9.2 °C under direct sunlight and promisingly reached the reduction of ∼7.4 °C even in hot, moist, and fickle extreme surroundings.					
35510868	7	9	dep	high-performance	1092:1107	arg1	thermal-regulating					1110:1127	thermal-regulating	1110:1127	thermal-regulating	1110:1127	This work can pave the way toward designing high-performance, thermal-regulating materials for energy consumption savings.					
35510868	2	10	theme	weather	321:327	arg1	conditions					329:338	different weather conditions	311:338	different weather conditions	311:338	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	3	11	theme	casting	578:584	arg1	process					586:592	unidirectional freeze casting process	556:592	unidirectional freeze casting process	556:592	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	3	12	theme	unidirectional	556:569	arg1	process					586:592	unidirectional freeze casting process	556:592	unidirectional freeze casting process	556:592	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	2	13	theme	different	311:319	arg1	conditions					329:338	different weather conditions	311:338	different weather conditions	311:338	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	3	14	theme	CNC	492:494	arg1	coolers					505:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	5	15	theme	cooling	804:810	arg1	performance					812:822	The tunable cooling performance	792:822	The tunable cooling performance	792:822	The tunable cooling performance can be realized via controlling the compression ratio of shape-malleable aerogel coolers.					
35510868	3	16	theme	chemical	529:536	arg1	cross-linking					538:550	chemical cross-linking	529:550	chemical cross-linking	529:550	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	3	17	theme	tunable	422:428	arg1	coolers					505:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	2	18	dep	cooler	281:286	arg1	meet					291:294	meet	291:294	to meet requirement of different weather conditions	288:338	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	5	19	theme	tunable	796:802	arg1	performance					812:822	The tunable cooling performance	792:822	The tunable cooling performance	792:822	The tunable cooling performance can be realized via controlling the compression ratio of shape-malleable aerogel coolers.					
35510868	6	20	theme	%	1017:1017	arg1	reduction					1019:1027	35.4% reduction	1013:1027	35.4% reduction of cooling energy	1013:1045	Furthermore, energy consumption modeling of using such aerogel coolers in buildings in China shows 35.4% reduction of cooling energy.					
35510868	4	21	theme	fickle	763:768	arg1	surroundings					778:789	hot, moist, and fickle extreme surroundings	747:789	hot, moist, and fickle extreme surroundings	747:789	Such aerogel coolers can achieve a subambient temperature drop of 9.2 °C under direct sunlight and promisingly reached the reduction of ∼7.4 °C even in hot, moist, and fickle extreme surroundings.					
35510868	7	22	theme	high-performance	1092:1107	arg1	materials					1129:1137	high-performance, thermal-regulating materials	1092:1137	high-performance, thermal-regulating materials	1092:1137	This work can pave the way toward designing high-performance, thermal-regulating materials for energy consumption savings.					
35510868	3	23	theme	insulating	441:450	arg1	coolers					505:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	0	24	theme	Tunable	12:18	arg1	Cellulose					40:48	Dynamically Tunable All-Weather Daytime Cellulose	0:48	Dynamically Tunable All-Weather Daytime Cellulose	0:48	Dynamically Tunable All-Weather Daytime Cellulose Aerogel Radiative Supercooler for Energy-Saving Building.					
35510868	4	25	theme	hot	747:749	arg1	surroundings					778:789	hot, moist, and fickle extreme surroundings	747:789	hot, moist, and fickle extreme surroundings	747:789	Such aerogel coolers can achieve a subambient temperature drop of 9.2 °C under direct sunlight and promisingly reached the reduction of ∼7.4 °C even in hot, moist, and fickle extreme surroundings.					
35510868	1	26	theme	overall	199:205	arg1	consumption					214:224	overall energy consumption	199:224	overall energy consumption	199:224	A passive cooling strategy without any electricity input has shown a significant impact on overall energy consumption globally.					
35510868	4	27	theme	°C	736:737	arg1	reduction					718:726	the reduction	714:726	the reduction of ∼7.4 °C	714:737	Such aerogel coolers can achieve a subambient temperature drop of 9.2 °C under direct sunlight and promisingly reached the reduction of ∼7.4 °C even in hot, moist, and fickle extreme surroundings.					
35510868	5	28	theme	compression	860:870	arg1	ratio					872:876	the compression ratio	856:876	the compression ratio of shape-malleable aerogel coolers	856:911	The tunable cooling performance can be realized via controlling the compression ratio of shape-malleable aerogel coolers.					
35510868	1	29	theme	passive	110:116	arg1	strategy					126:133	A passive cooling strategy	108:133	A passive cooling strategy without any electricity input	108:163	A passive cooling strategy without any electricity input has shown a significant impact on overall energy consumption globally.					
35510868	1	30	theme	energy	207:212	arg1	consumption					214:224	overall energy consumption	199:224	overall energy consumption	199:224	A passive cooling strategy without any electricity input has shown a significant impact on overall energy consumption globally.					
35510868	0	31	theme	Daytime	32:38	arg1	Cellulose					40:48	Dynamically Tunable All-Weather Daytime Cellulose	0:48	Dynamically Tunable All-Weather Daytime Cellulose	0:48	Dynamically Tunable All-Weather Daytime Cellulose Aerogel Radiative Supercooler for Energy-Saving Building.					
35510868	7	32	theme	energy	1143:1148	arg1	savings					1162:1168	energy consumption savings	1143:1168	energy consumption savings	1143:1168	This work can pave the way toward designing high-performance, thermal-regulating materials for energy consumption savings.					
35510868	1	33	theme	cooling	118:124	arg1	strategy					126:133	A passive cooling strategy	108:133	A passive cooling strategy without any electricity input	108:163	A passive cooling strategy without any electricity input has shown a significant impact on overall energy consumption globally.					
35510868	2	34	theme	daytime	263:269	arg1	cooler					281:286	designing tunable daytime radiative cooler	245:286	designing tunable daytime radiative cooler to meet requirement of different weather conditions	245:338	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	2	34	theme	daytime	263:269	arg1	challenge					355:363	a big challenge	349:363	a big challenge	349:363	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	3	35	theme	cellulose	469:477	arg1	coolers					505:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	0	36	theme	All-Weather	20:30	arg1	Cellulose					40:48	Dynamically Tunable All-Weather Daytime Cellulose	0:48	Dynamically Tunable All-Weather Daytime Cellulose	0:48	Dynamically Tunable All-Weather Daytime Cellulose Aerogel Radiative Supercooler for Energy-Saving Building.					
35510868	4	37	theme	extreme	770:776	arg1	surroundings					778:789	hot, moist, and fickle extreme surroundings	747:789	hot, moist, and fickle extreme surroundings	747:789	Such aerogel coolers can achieve a subambient temperature drop of 9.2 °C under direct sunlight and promisingly reached the reduction of ∼7.4 °C even in hot, moist, and fickle extreme surroundings.					
35510868	2	38	theme	conditions	329:338	arg1	requirement					296:306	requirement	296:306	requirement of different weather conditions	296:338	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	3	39	theme	nanocrystal	479:489	arg1	coolers					505:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	3	40	theme	coolers	505:511	arg1	type					414:417	a novel type	406:417	a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	406:511	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	0	41	dep	Supercooler	68:78	arg1	Building					98:105	Energy-Saving Building	84:105	Energy-Saving Building	84:105	Dynamically Tunable All-Weather Daytime Cellulose Aerogel Radiative Supercooler for Energy-Saving Building.					
35510868	0	41	dep	Supercooler	68:78	arg1	Cellulose					40:48	Dynamically Tunable All-Weather Daytime Cellulose	0:48	Dynamically Tunable All-Weather Daytime Cellulose	0:48	Dynamically Tunable All-Weather Daytime Cellulose Aerogel Radiative Supercooler for Energy-Saving Building.					
35510868	5	42	theme	shape-malleable	881:895	arg1	coolers					905:911	shape-malleable aerogel coolers	881:911	shape-malleable aerogel coolers	881:911	The tunable cooling performance can be realized via controlling the compression ratio of shape-malleable aerogel coolers.					
35510868	4	43	theme	°C	665:666	arg1	drop					653:656	a subambient temperature drop	628:656	a subambient temperature drop of 9.2 °C	628:666	Such aerogel coolers can achieve a subambient temperature drop of 9.2 °C under direct sunlight and promisingly reached the reduction of ∼7.4 °C even in hot, moist, and fickle extreme surroundings.					
35510868	4	44	theme	direct	674:679	arg1	sunlight					681:688	direct sunlight	674:688	direct sunlight	674:688	Such aerogel coolers can achieve a subambient temperature drop of 9.2 °C under direct sunlight and promisingly reached the reduction of ∼7.4 °C even in hot, moist, and fickle extreme surroundings.					
35510868	6	45	theme	aerogel	969:975	arg1	coolers					977:983	such aerogel coolers	964:983	such aerogel coolers	964:983	Furthermore, energy consumption modeling of using such aerogel coolers in buildings in China shows 35.4% reduction of cooling energy.					
35510868	6	46	from	buildings	988:996	arg1	China					1001:1005	China	1001:1005	China	1001:1005	Furthermore, energy consumption modeling of using such aerogel coolers in buildings in China shows 35.4% reduction of cooling energy.					
35510868	2	47	theme	tunable	255:261	arg1	cooler					281:286	designing tunable daytime radiative cooler	245:286	designing tunable daytime radiative cooler to meet requirement of different weather conditions	245:338	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	2	47	theme	tunable	255:261	arg1	challenge					355:363	a big challenge	349:363	a big challenge	349:363	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	3	48	theme	compressible	456:467	arg1	coolers					505:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	6	49	theme	such	964:967	arg1	coolers					977:983	such aerogel coolers	964:983	such aerogel coolers	964:983	Furthermore, energy consumption modeling of using such aerogel coolers in buildings in China shows 35.4% reduction of cooling energy.					
35510868	2	50	theme	designing	245:253	arg1	cooler					281:286	designing tunable daytime radiative cooler	245:286	designing tunable daytime radiative cooler to meet requirement of different weather conditions	245:338	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	2	50	theme	designing	245:253	arg1	challenge					355:363	a big challenge	349:363	a big challenge	349:363	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	3	51	theme	novel	408:412	arg1	type					414:417	a novel type	406:417	a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	406:511	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	3	52	theme	aerogel	497:503	arg1	coolers					505:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers	422:511	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	1	53	theme	electricity	147:157	arg1	input					159:163	any electricity input	143:163	any electricity input	143:163	A passive cooling strategy without any electricity input has shown a significant impact on overall energy consumption globally.					
35510868	3	54	theme	freeze	571:576	arg1	process					586:592	unidirectional freeze casting process	556:592	unidirectional freeze casting process	556:592	Here, a novel type of tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers is prepared via chemical cross-linking and unidirectional freeze casting process.					
35510868	6	55	theme	energy	1040:1045	arg1	reduction					1019:1027	35.4% reduction	1013:1027	35.4% reduction of cooling energy	1013:1045	Furthermore, energy consumption modeling of using such aerogel coolers in buildings in China shows 35.4% reduction of cooling energy.					
35510868	2	56	dep	hot	380:382	arg1	humid					385:389	humid	385:389	humid	385:389	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	2	57	theme	big	351:353	arg1	cooler					281:286	designing tunable daytime radiative cooler	245:286	designing tunable daytime radiative cooler to meet requirement of different weather conditions	245:338	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	2	57	theme	big	351:353	arg1	challenge					355:363	a big challenge	349:363	a big challenge	349:363	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	6	58	theme	cooling	1032:1038	arg1	energy					1040:1045	cooling energy	1032:1045	cooling energy	1032:1045	Furthermore, energy consumption modeling of using such aerogel coolers in buildings in China shows 35.4% reduction of cooling energy.					
35510868	0	59	theme	Energy-Saving	84:96	arg1	Building					98:105	Energy-Saving Building	84:105	Energy-Saving Building	84:105	Dynamically Tunable All-Weather Daytime Cellulose Aerogel Radiative Supercooler for Energy-Saving Building.					
35510868	5	60	theme	aerogel	897:903	arg1	coolers					905:911	shape-malleable aerogel coolers	881:911	shape-malleable aerogel coolers	881:911	The tunable cooling performance can be realized via controlling the compression ratio of shape-malleable aerogel coolers.					
35510868	4	61	theme	temperature	641:651	arg1	drop					653:656	a subambient temperature drop	628:656	a subambient temperature drop of 9.2 °C	628:666	Such aerogel coolers can achieve a subambient temperature drop of 9.2 °C under direct sunlight and promisingly reached the reduction of ∼7.4 °C even in hot, moist, and fickle extreme surroundings.					
35510868	2	62	theme	radiative	271:279	arg1	cooler					281:286	designing tunable daytime radiative cooler	245:286	designing tunable daytime radiative cooler to meet requirement of different weather conditions	245:338	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	2	62	theme	radiative	271:279	arg1	challenge					355:363	a big challenge	349:363	a big challenge	349:363	However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still a big challenge, especially in hot, humid regions.					
35510868	1	63	from	impact	189:194	arg1	consumption					214:224	overall energy consumption	199:224	overall energy consumption	199:224	A passive cooling strategy without any electricity input has shown a significant impact on overall energy consumption globally.					
35510868	5	64	theme	coolers	905:911	arg1	ratio					872:876	the compression ratio	856:876	the compression ratio of shape-malleable aerogel coolers	856:911	The tunable cooling performance can be realized via controlling the compression ratio of shape-malleable aerogel coolers.					
35510868	4	65	theme	subambient	630:639	arg1	drop					653:656	a subambient temperature drop	628:656	a subambient temperature drop of 9.2 °C	628:666	Such aerogel coolers can achieve a subambient temperature drop of 9.2 °C under direct sunlight and promisingly reached the reduction of ∼7.4 °C even in hot, moist, and fickle extreme surroundings.					
36841902	8	0	theme	microbial	1210:1218	arg1	assimilation					1220:1231	microbial assimilation	1210:1231	microbial assimilation	1210:1231	After 9 years, microbial assimilation became dominant, leading to chemical convergence, and fungi, particularly Chaetomium, were the main contributors to microbial assimilation.					
36841902	9	1	from	trajectory	1459:1468	arg1	chemistry					1481:1489	residue chemistry	1473:1489	residue chemistry	1473:1489	Overall, this study demonstrated that keystone taxa regulate the divergent-convergent trajectory in residue chemistry.					
36841902	8	2	theme	main	1328:1331	arg1	fungi					1287:1291	fungi	1287:1291	fungi	1287:1291	After 9 years, microbial assimilation became dominant, leading to chemical convergence, and fungi, particularly Chaetomium, were the main contributors to microbial assimilation.					
36841902	8	2	theme	main	1328:1331	arg1	contributors					1333:1344	the main contributors	1324:1344	the main contributors to microbial assimilation	1324:1370	After 9 years, microbial assimilation became dominant, leading to chemical convergence, and fungi, particularly Chaetomium, were the main contributors to microbial assimilation.					
36841902	3	3	theme	long-term	351:359	arg1	decomposition					361:373	long-term decomposition	351:373	long-term decomposition	351:373	There exist conflicting opinions on whether residue chemistry diverges or converges after long-term decomposition.					
36841902	5	4	theme	climate	642:648	arg1	zones					650:654	three climate zones	636:654	three climate zones	636:654	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	1	5	theme	mitigating	157:166	arg1	change					176:181	mitigating climate change	157:181	mitigating climate change	157:181	Managing above-ground plant carbon inputs can pave the way toward carbon neutrality and mitigating climate change.					
36841902	6	6	theme	type	847:850	arg1	effect					831:836	the combined effect	818:836	the combined effect of straw type and climate	818:862	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	4	7	theme	residue	433:439	arg1	chemistry					441:449	residue chemistry	433:449	residue chemistry	433:449	Moreover, whether and how microbial communities regulate residue chemistry remains unclear.					
36841902	7	8	theme	extracellular	1029:1041	arg1	activity					1050:1057	extracellular enzyme activity	1029:1057	extracellular enzyme activity	1029:1057	Chemical divergence during the first 2-3 years was primarily driven by the changes in extracellular enzyme activity influenced by keystone taxa-guided bacterial networks, and the keystone taxa belonged to Alphaproteobacteria, particularly Rhizobiales.					
36841902	9	9	theme	keystone	1411:1418	arg1	taxa					1420:1423	keystone taxa	1411:1423	keystone taxa	1411:1423	Overall, this study demonstrated that keystone taxa regulate the divergent-convergent trajectory in residue chemistry.					
36841902	7	10	theme	keystone	1073:1080	arg1	networks					1104:1111	keystone taxa-guided bacterial networks	1073:1111	keystone taxa-guided bacterial networks	1073:1111	Chemical divergence during the first 2-3 years was primarily driven by the changes in extracellular enzyme activity influenced by keystone taxa-guided bacterial networks, and the keystone taxa belonged to Alphaproteobacteria, particularly Rhizobiales.					
36841902	5	11	theme	maize	556:560	arg1	straw					562:566	maize straw	556:566	maize straw	556:566	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	5	12	from	years	627:631	arg1	zones					650:654	three climate zones	636:654	three climate zones	636:654	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	6	13	theme	climate	856:862	arg1	effect					831:836	the combined effect	818:836	the combined effect of straw type and climate	818:862	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	1	14	theme	Managing	69:76	arg1	inputs					104:109	Managing above-ground plant carbon inputs	69:109	Managing above-ground plant carbon inputs	69:109	Managing above-ground plant carbon inputs can pave the way toward carbon neutrality and mitigating climate change.					
36841902	5	15	theme	straw	562:566	arg1	dynamics					544:551	residue composition dynamics	524:551	residue composition dynamics	524:551	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	5	15	theme	straw	562:566	arg1	microbiomes					596:606	related microbiomes	588:606	related microbiomes	588:606	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	5	15	theme	straw	562:566	arg1	processes					510:518	decomposition processes	496:518	decomposition processes	496:518	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	1	16	theme	climate	168:174	arg1	change					176:181	mitigating climate change	157:181	mitigating climate change	157:181	Managing above-ground plant carbon inputs can pave the way toward carbon neutrality and mitigating climate change.					
36841902	6	17	theme	year	930:933	arg1	period					935:940	the 3-9 year period	922:940	the 3-9 year period	922:940	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	3	18	theme	conflicting	273:283	arg1	opinions					285:292	conflicting opinions	273:292	conflicting opinions	273:292	There exist conflicting opinions on whether residue chemistry diverges or converges after long-term decomposition.					
36841902	4	19	theme	microbial	402:410	arg1	communities					412:422	microbial communities	402:422	microbial communities	402:422	Moreover, whether and how microbial communities regulate residue chemistry remains unclear.					
36841902	1	20	theme	above-ground	78:89	arg1	inputs					104:109	Managing above-ground plant carbon inputs	69:109	Managing above-ground plant carbon inputs	69:109	Managing above-ground plant carbon inputs can pave the way toward carbon neutrality and mitigating climate change.					
36841902	0	21	theme	keystone	10:17	arg1	taxa					19:22	Microbial keystone taxa	0:22	Microbial keystone taxa	0:22	Microbial keystone taxa drive succession of plant residue chemistry.					
36841902	6	22	theme	3-9	926:928	arg1	year					930:933	year	930:933	year	930:933	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	5	23	theme	years	627:631	arg1	period					615:620	a period	613:620	a period of 9 years in three climate zones	613:654	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	1	24	theme	plant	91:95	arg1	inputs					104:109	Managing above-ground plant carbon inputs	69:109	Managing above-ground plant carbon inputs	69:109	Managing above-ground plant carbon inputs can pave the way toward carbon neutrality and mitigating climate change.					
36841902	5	25	theme	wheat	572:576	arg1	straw					578:582	wheat straw	572:582	wheat straw	572:582	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	0	26	theme	Microbial	0:8	arg1	taxa					19:22	Microbial keystone taxa	0:22	Microbial keystone taxa	0:22	Microbial keystone taxa drive succession of plant residue chemistry.					
36841902	6	27	theme	straw	841:845	arg1	type					847:850	straw type	841:850	straw type	841:850	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	1	28	theme	carbon	97:102	arg1	inputs					104:109	Managing above-ground plant carbon inputs	69:109	Managing above-ground plant carbon inputs	69:109	Managing above-ground plant carbon inputs can pave the way toward carbon neutrality and mitigating climate change.					
36841902	5	29	theme	residue	524:530	arg1	dynamics					544:551	residue composition dynamics	524:551	residue composition dynamics	524:551	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	6	30	dep	composition	762:772	arg1	diverged					774:781	diverged	774:781	diverged during the 0.5-3 year period under the combined effect of straw type and climate	774:862	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	6	30	dep	composition	762:772	arg1	converged					873:881	converged	873:881	then converged to an array of common compounds during the 3-9 year period	868:940	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	7	31	theme	first	974:978	arg1	years					984:988	the first 2-3 years	970:988	the first 2-3 years	970:988	Chemical divergence during the first 2-3 years was primarily driven by the changes in extracellular enzyme activity influenced by keystone taxa-guided bacterial networks, and the keystone taxa belonged to Alphaproteobacteria, particularly Rhizobiales.					
36841902	7	32	theme	keystone	1122:1129	arg1	taxa					1131:1134	the keystone taxa	1118:1134	the keystone taxa	1118:1134	Chemical divergence during the first 2-3 years was primarily driven by the changes in extracellular enzyme activity influenced by keystone taxa-guided bacterial networks, and the keystone taxa belonged to Alphaproteobacteria, particularly Rhizobiales.					
36841902	4	33	dep	whether	386:392	arg1	regulate					424:431	regulate	424:431	regulate residue chemistry	424:449	Moreover, whether and how microbial communities regulate residue chemistry remains unclear.					
36841902	5	34	from	period	615:620	arg1	zones					650:654	three climate zones	636:654	three climate zones	636:654	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	6	35	theme	year	800:803	arg1	period					805:810	the 0.5-3 year period	790:810	the 0.5-3 year period	790:810	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	3	36	theme	residue	305:311	arg1	chemistry					313:321	residue chemistry	305:321	residue chemistry	305:321	There exist conflicting opinions on whether residue chemistry diverges or converges after long-term decomposition.					
36841902	2	37	theme	Chemical	184:191	arg1	complexity					193:202	Chemical complexity	184:202	Chemical complexity of plant residues	184:220	Chemical complexity of plant residues largely controls carbon sequestration.					
36841902	5	38	from	zones	650:654	arg1	period					615:620	a period	613:620	a period of 9 years in three climate zones	613:654	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	7	39	from	changes	1018:1024	arg1	activity					1050:1057	extracellular enzyme activity	1029:1057	extracellular enzyme activity	1029:1057	Chemical divergence during the first 2-3 years was primarily driven by the changes in extracellular enzyme activity influenced by keystone taxa-guided bacterial networks, and the keystone taxa belonged to Alphaproteobacteria, particularly Rhizobiales.					
36841902	6	40	theme	residue	754:760	arg1	composition					762:772	the residue composition	750:772	the residue composition	750:772	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	6	40	theme	residue	754:760	arg1	trajectory					708:717	a divergent-convergent trajectory	685:717	a divergent-convergent trajectory	685:717	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	6	41	theme	compounds	905:913	arg1	array					889:893	an array	886:893	an array of common compounds	886:913	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	2	42	theme	carbon	239:244	arg1	sequestration					246:258	carbon sequestration	239:258	carbon sequestration	239:258	Chemical complexity of plant residues largely controls carbon sequestration.					
36841902	0	43	theme	residue	50:56	arg1	chemistry					58:66	plant residue chemistry	44:66	plant residue chemistry	44:66	Microbial keystone taxa drive succession of plant residue chemistry.					
36841902	9	44	theme	divergent-convergent	1438:1457	arg1	trajectory					1459:1468	the divergent-convergent trajectory	1434:1468	the divergent-convergent trajectory in residue chemistry	1434:1489	Overall, this study demonstrated that keystone taxa regulate the divergent-convergent trajectory in residue chemistry.					
36841902	6	45	theme	common	898:903	arg1	compounds					905:913	common compounds	898:913	common compounds	898:913	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	6	46	theme	combined	822:829	arg1	effect					831:836	the combined effect	818:836	the combined effect of straw type and climate	818:862	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	0	47	theme	plant	44:48	arg1	chemistry					58:66	plant residue chemistry	44:66	plant residue chemistry	44:66	Microbial keystone taxa drive succession of plant residue chemistry.					
36841902	2	48	theme	residues	213:220	arg1	complexity					193:202	Chemical complexity	184:202	Chemical complexity of plant residues	184:220	Chemical complexity of plant residues largely controls carbon sequestration.					
36841902	8	49	theme	microbial	1349:1357	arg1	assimilation					1359:1370	microbial assimilation	1349:1370	microbial assimilation	1349:1370	After 9 years, microbial assimilation became dominant, leading to chemical convergence, and fungi, particularly Chaetomium, were the main contributors to microbial assimilation.					
36841902	7	50	theme	enzyme	1043:1048	arg1	activity					1050:1057	extracellular enzyme activity	1029:1057	extracellular enzyme activity	1029:1057	Chemical divergence during the first 2-3 years was primarily driven by the changes in extracellular enzyme activity influenced by keystone taxa-guided bacterial networks, and the keystone taxa belonged to Alphaproteobacteria, particularly Rhizobiales.					
36841902	7	51	theme	taxa-guided	1082:1092	arg1	networks					1104:1111	keystone taxa-guided bacterial networks	1073:1111	keystone taxa-guided bacterial networks	1073:1111	Chemical divergence during the first 2-3 years was primarily driven by the changes in extracellular enzyme activity influenced by keystone taxa-guided bacterial networks, and the keystone taxa belonged to Alphaproteobacteria, particularly Rhizobiales.					
36841902	2	52	theme	plant	207:211	arg1	residues					213:220	plant residues	207:220	plant residues	207:220	Chemical complexity of plant residues largely controls carbon sequestration.					
36841902	5	53	theme	decomposition	496:508	arg1	processes					510:518	decomposition processes	496:518	decomposition processes	496:518	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	9	54	theme	residue	1473:1479	arg1	chemistry					1481:1489	residue chemistry	1473:1489	residue chemistry	1473:1489	Overall, this study demonstrated that keystone taxa regulate the divergent-convergent trajectory in residue chemistry.					
36841902	7	55	theme	bacterial	1094:1102	arg1	networks					1104:1111	keystone taxa-guided bacterial networks	1073:1111	keystone taxa-guided bacterial networks	1073:1111	Chemical divergence during the first 2-3 years was primarily driven by the changes in extracellular enzyme activity influenced by keystone taxa-guided bacterial networks, and the keystone taxa belonged to Alphaproteobacteria, particularly Rhizobiales.					
36841902	6	56	theme	0.5-3	794:798	arg1	year					800:803	year	800:803	year	800:803	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	5	57	theme	straw	578:582	arg1	dynamics					544:551	residue composition dynamics	524:551	residue composition dynamics	524:551	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	5	57	theme	straw	578:582	arg1	microbiomes					596:606	related microbiomes	588:606	related microbiomes	588:606	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	5	57	theme	straw	578:582	arg1	processes					510:518	decomposition processes	496:518	decomposition processes	496:518	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	5	58	theme	composition	532:542	arg1	dynamics					544:551	residue composition dynamics	524:551	residue composition dynamics	524:551	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	7	59	theme	Chemical	943:950	arg1	divergence					952:961	Chemical divergence	943:961	Chemical divergence during the first 2-3 years	943:988	Chemical divergence during the first 2-3 years was primarily driven by the changes in extracellular enzyme activity influenced by keystone taxa-guided bacterial networks, and the keystone taxa belonged to Alphaproteobacteria, particularly Rhizobiales.					
36841902	6	60	theme	divergent-convergent	687:706	arg1	composition					762:772	the residue composition	750:772	the residue composition	750:772	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	6	60	theme	divergent-convergent	687:706	arg1	trajectory					708:717	a divergent-convergent trajectory	685:717	a divergent-convergent trajectory	685:717	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	1	61	theme	carbon	135:140	arg1	neutrality					142:151	carbon neutrality	135:151	carbon neutrality	135:151	Managing above-ground plant carbon inputs can pave the way toward carbon neutrality and mitigating climate change.					
36841902	0	62	theme	chemistry	58:66	arg1	succession					30:39	succession	30:39	succession of plant residue chemistry	30:66	Microbial keystone taxa drive succession of plant residue chemistry.					
36841902	8	63	theme	chemical	1261:1268	arg1	convergence					1270:1280	chemical convergence	1261:1280	chemical convergence	1261:1280	After 9 years, microbial assimilation became dominant, leading to chemical convergence, and fungi, particularly Chaetomium, were the main contributors to microbial assimilation.					
36841902	5	64	theme	related	588:594	arg1	microbiomes					596:606	related microbiomes	588:606	related microbiomes	588:606	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36841902	6	65	theme	Residue	657:663	arg1	chemistry					665:673	Residue chemistry	657:673	Residue chemistry	657:673	Residue chemistry exhibited a divergent-convergent trajectory during decomposition, that is, the residue composition diverged during the 0.5-3 year period under the combined effect of straw type and climate and then converged to an array of common compounds during the 3-9 year period.					
36841902	5	66	dep	processes	510:518	arg1	the					492:494	the	492:494	the	492:494	This study investigated the decomposition processes and residue composition dynamics of maize straw and wheat straw and related microbiomes over a period of 9 years in three climate zones.					
36194910	2	0	theme	approved	484:491	arg1	HIV/AIDS					475:482	HIV/AIDS	475:482	HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021	475:551	Cabotegravir is an antiretroviral medication used for the treatment of HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021.					
36194910	8	1	theme	RR	1397:1398	arg1	µgmL-1					1443:1448	≤ 0.02 and 0.03 µgmL-1	1427:1448	≤ 0.02 and 0.03 µgmL-1	1427:1448	The limits of detection and quantification for cabotegravir and its enantiomer (RS isomer) were ≤ 0.02 and ≤ 0.06, and the RR and SS-isomers limits were ≤ 0.02 and 0.03 µgmL-1 respectively.					
36194910	8	1	theme	RR	1397:1398	arg1	limits					1415:1420	the RR and SS-isomers limits	1393:1420	the RR and SS-isomers limits	1393:1420	The limits of detection and quantification for cabotegravir and its enantiomer (RS isomer) were ≤ 0.02 and ≤ 0.06, and the RR and SS-isomers limits were ≤ 0.02 and 0.03 µgmL-1 respectively.					
36194910	0	2	theme	coated	161:166	arg1	phase					202:206	coated cellulose-based chiral stationary phase	161:206	coated cellulose-based chiral stationary phase	161:206	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	3	3	theme	HILIC	696:700	arg1	mode					702:705	HILIC mode	696:705	HILIC mode	696:705	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	1	4	theme	first	235:239	arg1	method					270:275	The first, sensitive, and rapid chiral method	231:275	The first, sensitive, and rapid chiral method	231:275	The first, sensitive, and rapid chiral method was developed for enatioseperations and determination of cabotegravir and its enantiomeric impurities by using HPLC and LC-MS.					
36194910	2	5	theme	HIV/AIDS	475:482	arg1	treatment					462:470	the treatment	458:470	the treatment of HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021	458:551	Cabotegravir is an antiretroviral medication used for the treatment of HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021.					
36194910	3	6	theme	CHIRALCEL	669:677	arg1	OX-3R					679:683	CHIRALCEL OX-3R	669:683	CHIRALCEL OX-3R	669:683	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	3	6	theme	CHIRALCEL	669:677	arg1	cellulose-tris					616:629	the coated cellulose-tris	605:629	the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode	605:705	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	4	7	theme	organic	824:830	arg1	modifier					832:839	organic modifier	824:839	organic modifier	824:839	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	8	8	theme	SS-isomers	1404:1413	arg1	µgmL-1					1443:1448	≤ 0.02 and 0.03 µgmL-1	1427:1448	≤ 0.02 and 0.03 µgmL-1	1427:1448	The limits of detection and quantification for cabotegravir and its enantiomer (RS isomer) were ≤ 0.02 and ≤ 0.06, and the RR and SS-isomers limits were ≤ 0.02 and 0.03 µgmL-1 respectively.					
36194910	8	8	theme	SS-isomers	1404:1413	arg1	limits					1415:1420	the RR and SS-isomers limits	1393:1420	the RR and SS-isomers limits	1393:1420	The limits of detection and quantification for cabotegravir and its enantiomer (RS isomer) were ≤ 0.02 and ≤ 0.06, and the RR and SS-isomers limits were ≤ 0.02 and 0.03 µgmL-1 respectively.					
36194910	0	9	theme	cabotegravir	98:109	arg1	RS					123:124	cabotegravir and related RS	98:124	cabotegravir and related RS	98:124	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	1	10	theme	cabotegravir	334:345	arg1	determination					317:329	determination	317:329	determination	317:329	The first, sensitive, and rapid chiral method was developed for enatioseperations and determination of cabotegravir and its enantiomeric impurities by using HPLC and LC-MS.					
36194910	1	10	theme	cabotegravir	334:345	arg1	enatioseperations					295:311	enatioseperations	295:311	enatioseperations	295:311	The first, sensitive, and rapid chiral method was developed for enatioseperations and determination of cabotegravir and its enantiomeric impurities by using HPLC and LC-MS.					
36194910	0	11	theme	chiral	184:189	arg1	phase					202:206	coated cellulose-based chiral stationary phase	161:206	coated cellulose-based chiral stationary phase	161:206	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	10	12	from	determination	1594:1606	arg1	drugs					1682:1686	the bulk drugs	1673:1686	the bulk drugs	1673:1686	Finally, the validated method was applied for the determination and identification of cabotegravir and its chiral enantiomers in the bulk drugs by using HPLC and LC-MS techniques.					
36194910	1	13	theme	sensitive	242:250	arg1	method					270:275	The first, sensitive, and rapid chiral method	231:275	The first, sensitive, and rapid chiral method	231:275	The first, sensitive, and rapid chiral method was developed for enatioseperations and determination of cabotegravir and its enantiomeric impurities by using HPLC and LC-MS.					
36194910	5	14	theme	%	1038:1038	arg1	addition					1046:1053	0.1% (v/v) addition	1035:1053	0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin-1	1035:1109	The mobile phase consisted of acetonitrile and water with 0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin-1.					
36194910	0	15	theme	cellulose-based	168:182	arg1	phase					202:206	coated cellulose-based chiral stationary phase	161:206	coated cellulose-based chiral stationary phase	161:206	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	5	16	with	additive	1070:1077	arg1	rate					1093:1096	the flow rate	1084:1096	the flow rate of 1 mLmin-1	1084:1109	The mobile phase consisted of acetonitrile and water with 0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin-1.					
36194910	10	17	theme	chiral	1651:1656	arg1	enantiomers					1658:1668	its chiral enantiomers	1647:1668	its chiral enantiomers in the bulk drugs	1647:1686	Finally, the validated method was applied for the determination and identification of cabotegravir and its chiral enantiomers in the bulk drugs by using HPLC and LC-MS techniques.					
36194910	0	18	theme	related	115:121	arg1	RS					123:124	cabotegravir and related RS	98:124	cabotegravir and related RS	98:124	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	7	19	theme	0.04-1.125 µgmL-1	1255:1271	arg1	range					1246:1250	the concentration range	1228:1250	the concentration range of 0.04-1.125 µgmL-1	1228:1271	The calibration curves of cabotegravir and its enantiomers were linear over the concentration range of 0.04-1.125 µgmL-1.					
36194910	5	20	theme	0.1	1035:1037	arg1	%					1038:1038	%	1038:1038	%	1038:1038	The mobile phase consisted of acetonitrile and water with 0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin-1.					
36194910	4	21	theme	temperature	900:910	arg1	effects					755:761	The effects	751:761	The effects of mobile phase composition	751:789	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	21	theme	temperature	900:910	arg1	effect					856:861	the effect	852:861	the effect of mobile phase-additives and column temperature	852:910	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	21	theme	temperature	900:910	arg1	mode					800:803	elution mode	792:803	elution mode	792:803	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	21	theme	temperature	900:910	arg1	percentage					810:819	percentage	810:819	percentage of organic modifier	810:839	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	1	22	theme	enantiomeric	355:366	arg1	impurities					368:377	its enantiomeric impurities	351:377	its enantiomeric impurities	351:377	The first, sensitive, and rapid chiral method was developed for enatioseperations and determination of cabotegravir and its enantiomeric impurities by using HPLC and LC-MS.					
36194910	0	23	theme	stationary	191:200	arg1	phase					202:206	coated cellulose-based chiral stationary phase	161:206	coated cellulose-based chiral stationary phase	161:206	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	10	24	from	identification	1612:1625	arg1	drugs					1682:1686	the bulk drugs	1673:1686	the bulk drugs	1673:1686	Finally, the validated method was applied for the determination and identification of cabotegravir and its chiral enantiomers in the bulk drugs by using HPLC and LC-MS techniques.					
36194910	5	25	dep	%	1038:1038	arg1	v/v					1041:1043	v/v	1041:1043	v/v	1041:1043	The mobile phase consisted of acetonitrile and water with 0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin-1.					
36194910	0	26	theme	LC	7:8	arg1	development					17:27	Chiral LC method development	0:27	Chiral LC method development	0:27	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	1	27	theme	impurities	368:377	arg1	determination					317:329	determination	317:329	determination	317:329	The first, sensitive, and rapid chiral method was developed for enatioseperations and determination of cabotegravir and its enantiomeric impurities by using HPLC and LC-MS.					
36194910	1	27	theme	impurities	368:377	arg1	enatioseperations					295:311	enatioseperations	295:311	enatioseperations	295:311	The first, sensitive, and rapid chiral method was developed for enatioseperations and determination of cabotegravir and its enantiomeric impurities by using HPLC and LC-MS.					
36194910	8	28	theme	detection	1288:1296	arg1	≤ 0.02					1370:1375	≤ 0.02	1370:1375	≤ 0.02	1370:1375	The limits of detection and quantification for cabotegravir and its enantiomer (RS isomer) were ≤ 0.02 and ≤ 0.06, and the RR and SS-isomers limits were ≤ 0.02 and 0.03 µgmL-1 respectively.					
36194910	8	28	theme	detection	1288:1296	arg1	limits					1278:1283	The limits	1274:1283	The limits of detection and quantification for cabotegravir and its enantiomer (RS isomer)	1274:1363	The limits of detection and quantification for cabotegravir and its enantiomer (RS isomer) were ≤ 0.02 and ≤ 0.06, and the RR and SS-isomers limits were ≤ 0.02 and 0.03 µgmL-1 respectively.					
36194910	9	29	theme	proposed	1493:1500	arg1	selective					1512:1520	selective	1512:1520	selective	1512:1520	It was demonstrated that the proposed method is selective, precise, and robust.					
36194910	9	29	theme	proposed	1493:1500	arg1	method					1502:1507	the proposed method	1489:1507	the proposed method	1489:1507	It was demonstrated that the proposed method is selective, precise, and robust.					
36194910	0	30	theme	Chiral	0:5	arg1	development					17:27	Chiral LC method development	0:27	Chiral LC method development	0:27	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	3	31	theme	run	721:723	arg1	less					733:736	less	733:736	less	733:736	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	3	31	theme	run	721:723	arg1	time					725:728	the total run time	711:728	the total run time	711:728	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	10	32	theme	LC-MS	1706:1710	arg1	techniques					1712:1721	HPLC and LC-MS techniques	1697:1721	HPLC and LC-MS techniques	1697:1721	Finally, the validated method was applied for the determination and identification of cabotegravir and its chiral enantiomers in the bulk drugs by using HPLC and LC-MS techniques.					
36194910	10	33	theme	bulk	1677:1680	arg1	drugs					1682:1686	the bulk drugs	1673:1686	the bulk drugs	1673:1686	Finally, the validated method was applied for the determination and identification of cabotegravir and its chiral enantiomers in the bulk drugs by using HPLC and LC-MS techniques.					
36194910	7	34	theme	concentration	1232:1244	arg1	range					1246:1250	the concentration range	1228:1250	the concentration range of 0.04-1.125 µgmL-1	1228:1271	The calibration curves of cabotegravir and its enantiomers were linear over the concentration range of 0.04-1.125 µgmL-1.					
36194910	2	35	theme	antiretroviral	423:436	arg1	medication					438:447	an antiretroviral medication	420:447	an antiretroviral medication used for the treatment of HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021	420:551	Cabotegravir is an antiretroviral medication used for the treatment of HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021.					
36194910	2	35	theme	antiretroviral	423:436	arg1	Cabotegravir					404:415	Cabotegravir	404:415	Cabotegravir	404:415	Cabotegravir is an antiretroviral medication used for the treatment of HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021.					
36194910	1	36	theme	rapid	257:261	arg1	method					270:275	The first, sensitive, and rapid chiral method	231:275	The first, sensitive, and rapid chiral method	231:275	The first, sensitive, and rapid chiral method was developed for enatioseperations and determination of cabotegravir and its enantiomeric impurities by using HPLC and LC-MS.					
36194910	2	37	dep	food	500:503	arg1	the					496:498	the	496:498	the	496:498	Cabotegravir is an antiretroviral medication used for the treatment of HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021.					
36194910	2	37	dep	food	500:503	arg1	FDA					531:533	FDA	531:533	FDA	531:533	Cabotegravir is an antiretroviral medication used for the treatment of HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021.					
36194910	2	37	dep	food	500:503	arg1	administration					515:528	administration	515:528	administration (FDA)	515:534	Cabotegravir is an antiretroviral medication used for the treatment of HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021.					
36194910	4	38	theme	composition	779:789	arg1	effects					755:761	The effects	751:761	The effects of mobile phase composition	751:789	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	38	theme	composition	779:789	arg1	effect					856:861	the effect	852:861	the effect of mobile phase-additives and column temperature	852:910	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	38	theme	composition	779:789	arg1	mode					800:803	elution mode	792:803	elution mode	792:803	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	38	theme	composition	779:789	arg1	percentage					810:819	percentage	810:819	percentage of organic modifier	810:839	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	8	39	theme	quantification	1302:1315	arg1	≤ 0.02					1370:1375	≤ 0.02	1370:1375	≤ 0.02	1370:1375	The limits of detection and quantification for cabotegravir and its enantiomer (RS isomer) were ≤ 0.02 and ≤ 0.06, and the RR and SS-isomers limits were ≤ 0.02 and 0.03 µgmL-1 respectively.					
36194910	8	39	theme	quantification	1302:1315	arg1	limits					1278:1283	The limits	1274:1283	The limits of detection and quantification for cabotegravir and its enantiomer (RS isomer)	1274:1363	The limits of detection and quantification for cabotegravir and its enantiomer (RS isomer) were ≤ 0.02 and ≤ 0.06, and the RR and SS-isomers limits were ≤ 0.02 and 0.03 µgmL-1 respectively.					
36194910	4	40	theme	phase	773:777	arg1	composition					779:789	mobile phase composition	766:789	mobile phase composition	766:789	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	0	41	theme	method	10:15	arg1	development					17:27	Chiral LC method development	0:27	Chiral LC method development	0:27	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	1	42	theme	chiral	263:268	arg1	method					270:275	The first, sensitive, and rapid chiral method	231:275	The first, sensitive, and rapid chiral method	231:275	The first, sensitive, and rapid chiral method was developed for enatioseperations and determination of cabotegravir and its enantiomeric impurities by using HPLC and LC-MS.					
36194910	0	43	theme	RS	123:124	arg1	characterization					59:74	characterization	59:74	characterization	59:74	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	0	43	theme	RS	123:124	arg1	development					17:27	Chiral LC method development	0:27	Chiral LC method development	0:27	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	0	43	theme	RS	123:124	arg1	separation					47:56	Stereo-selective separation	30:56	Stereo-selective separation	30:56	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	0	43	theme	RS	123:124	arg1	determination					81:93	determination	81:93	determination	81:93	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	4	44	theme	mobile	866:871	arg1	phase-additives					873:887	mobile phase-additives	866:887	mobile phase-additives	866:887	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	6	45	theme	UV	1112:1113	arg1	detection					1115:1123	UV detection	1112:1123	UV detection	1112:1123	UV detection was carried out at 220 nm.					
36194910	10	46	theme	cabotegravir	1630:1641	arg1	identification					1612:1625	identification	1612:1625	identification	1612:1625	Finally, the validated method was applied for the determination and identification of cabotegravir and its chiral enantiomers in the bulk drugs by using HPLC and LC-MS techniques.					
36194910	10	46	theme	cabotegravir	1630:1641	arg1	determination					1594:1606	determination	1594:1606	determination	1594:1606	Finally, the validated method was applied for the determination and identification of cabotegravir and its chiral enantiomers in the bulk drugs by using HPLC and LC-MS techniques.					
36194910	10	46	theme	cabotegravir	1630:1641	arg1	enantiomers					1658:1668	its chiral enantiomers	1647:1668	its chiral enantiomers in the bulk drugs	1647:1686	Finally, the validated method was applied for the determination and identification of cabotegravir and its chiral enantiomers in the bulk drugs by using HPLC and LC-MS techniques.					
36194910	0	47	theme	Stereo-selective	30:45	arg1	separation					47:56	Stereo-selective separation	30:56	Stereo-selective separation	30:56	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	3	48	theme	total	715:719	arg1	less					733:736	less	733:736	less	733:736	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	3	48	theme	total	715:719	arg1	time					725:728	the total run time	711:728	the total run time	711:728	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	4	49	theme	phase-additives	873:887	arg1	effects					755:761	The effects	751:761	The effects of mobile phase composition	751:789	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	49	theme	phase-additives	873:887	arg1	effect					856:861	the effect	852:861	the effect of mobile phase-additives and column temperature	852:910	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	49	theme	phase-additives	873:887	arg1	mode					800:803	elution mode	792:803	elution mode	792:803	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	49	theme	phase-additives	873:887	arg1	percentage					810:819	percentage	810:819	percentage of organic modifier	810:839	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	3	50	theme	4-chloro-3-methyl	632:648	arg1	carbamate					657:665	4-chloro-3-methyl phenyl carbamate	632:665	4-chloro-3-methyl phenyl carbamate	632:665	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	3	50	theme	4-chloro-3-methyl	632:648	arg1	cellulose-tris					616:629	the coated cellulose-tris	605:629	the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode	605:705	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	3	51	theme	chiral	571:576	arg1	separation					578:587	The cabotegravir chiral separation	554:587	The cabotegravir chiral separation	554:587	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	7	52	theme	enantiomers	1199:1209	arg1	linear					1216:1221	linear	1216:1221	linear	1216:1221	The calibration curves of cabotegravir and its enantiomers were linear over the concentration range of 0.04-1.125 µgmL-1.					
36194910	7	52	theme	enantiomers	1199:1209	arg1	curves					1168:1173	The calibration curves	1152:1173	The calibration curves of cabotegravir and its enantiomers	1152:1209	The calibration curves of cabotegravir and its enantiomers were linear over the concentration range of 0.04-1.125 µgmL-1.					
36194910	5	53	theme	additive	1070:1077	arg1	acid					1065:1068	formic acid	1058:1068	formic acid additive with the flow rate of 1 mLmin-1	1058:1109	The mobile phase consisted of acetonitrile and water with 0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin-1.					
36194910	0	54	theme	RR	127:128	arg1	characterization					59:74	characterization	59:74	characterization	59:74	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	0	54	theme	RR	127:128	arg1	development					17:27	Chiral LC method development	0:27	Chiral LC method development	0:27	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	0	54	theme	RR	127:128	arg1	separation					47:56	Stereo-selective separation	30:56	Stereo-selective separation	30:56	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	0	54	theme	RR	127:128	arg1	determination					81:93	determination	81:93	determination	81:93	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	3	55	theme	coated	609:614	arg1	carbamate					657:665	4-chloro-3-methyl phenyl carbamate	632:665	4-chloro-3-methyl phenyl carbamate	632:665	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	3	55	theme	coated	609:614	arg1	cellulose-tris					616:629	the coated cellulose-tris	605:629	the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode	605:705	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	3	55	theme	coated	609:614	arg1	OX-3R					679:683	CHIRALCEL OX-3R	669:683	CHIRALCEL OX-3R	669:683	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	4	56	theme	mobile	766:771	arg1	composition					779:789	mobile phase composition	766:789	mobile phase composition	766:789	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	5	57	theme	mLmin-1	1103:1109	arg1	rate					1093:1096	the flow rate	1084:1096	the flow rate of 1 mLmin-1	1084:1109	The mobile phase consisted of acetonitrile and water with 0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin-1.					
36194910	4	58	theme	peak	962:965	arg1	symmetry					967:974	peak symmetry	962:974	peak symmetry	962:974	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	59	theme	column	893:898	arg1	temperature					900:910	column temperature	893:910	column temperature	893:910	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	0	60	theme	SS	135:136	arg1	isomeric					138:145	SS isomeric	135:145	SS isomeric	135:145	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	3	61	theme	cellulose-tris	616:629	arg1	column					686:691	the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column	605:691	the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode	605:705	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	5	62	theme	formic	1058:1063	arg1	acid					1065:1068	formic acid	1058:1068	formic acid additive with the flow rate of 1 mLmin-1	1058:1109	The mobile phase consisted of acetonitrile and water with 0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin-1.					
36194910	3	63	from	column	686:691	arg1	mode					702:705	HILIC mode	696:705	HILIC mode	696:705	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	4	64	theme	elution	792:798	arg1	mode					800:803	elution mode	792:803	elution mode	792:803	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	5	65	theme	mobile	981:986	arg1	phase					988:992	The mobile phase	977:992	The mobile phase	977:992	The mobile phase consisted of acetonitrile and water with 0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin-1.					
36194910	3	66	theme	cabotegravir	558:569	arg1	separation					578:587	The cabotegravir chiral separation	554:587	The cabotegravir chiral separation	554:587	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	4	67	theme	modifier	832:839	arg1	effects					755:761	The effects	751:761	The effects of mobile phase composition	751:789	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	67	theme	modifier	832:839	arg1	effect					856:861	the effect	852:861	the effect of mobile phase-additives and column temperature	852:910	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	67	theme	modifier	832:839	arg1	mode					800:803	elution mode	792:803	elution mode	792:803	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	4	67	theme	modifier	832:839	arg1	percentage					810:819	percentage	810:819	percentage of organic modifier	810:839	The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry.					
36194910	5	68	theme	flow	1088:1091	arg1	rate					1093:1096	the flow rate	1084:1096	the flow rate of 1 mLmin-1	1084:1109	The mobile phase consisted of acetonitrile and water with 0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin-1.					
36194910	2	69	from	food	500:503	arg1	year					543:546	the year 2021	539:551	the year 2021	539:551	Cabotegravir is an antiretroviral medication used for the treatment of HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021.					
36194910	10	70	theme	HPLC	1697:1700	arg1	techniques					1712:1721	HPLC and LC-MS techniques	1697:1721	HPLC and LC-MS techniques	1697:1721	Finally, the validated method was applied for the determination and identification of cabotegravir and its chiral enantiomers in the bulk drugs by using HPLC and LC-MS techniques.					
36194910	10	71	dep	determination	1594:1606	arg1	the					1590:1592	the	1590:1592	the	1590:1592	Finally, the validated method was applied for the determination and identification of cabotegravir and its chiral enantiomers in the bulk drugs by using HPLC and LC-MS techniques.					
36194910	0	72	theme	isomeric	138:145	arg1	characterization					59:74	characterization	59:74	characterization	59:74	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	0	72	theme	isomeric	138:145	arg1	development					17:27	Chiral LC method development	0:27	Chiral LC method development	0:27	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	0	72	theme	isomeric	138:145	arg1	separation					47:56	Stereo-selective separation	30:56	Stereo-selective separation	30:56	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	0	72	theme	isomeric	138:145	arg1	determination					81:93	determination	81:93	determination	81:93	Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS.					
36194910	3	73	theme	phenyl	650:655	arg1	carbamate					657:665	4-chloro-3-methyl phenyl carbamate	632:665	4-chloro-3-methyl phenyl carbamate	632:665	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	3	73	theme	phenyl	650:655	arg1	cellulose-tris					616:629	the coated cellulose-tris	605:629	the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode	605:705	The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min.					
36194910	7	74	theme	cabotegravir	1178:1189	arg1	linear					1216:1221	linear	1216:1221	linear	1216:1221	The calibration curves of cabotegravir and its enantiomers were linear over the concentration range of 0.04-1.125 µgmL-1.					
36194910	7	74	theme	cabotegravir	1178:1189	arg1	curves					1168:1173	The calibration curves	1152:1173	The calibration curves of cabotegravir and its enantiomers	1152:1209	The calibration curves of cabotegravir and its enantiomers were linear over the concentration range of 0.04-1.125 µgmL-1.					
36194910	10	75	from	enantiomers	1658:1668	arg1	drugs					1682:1686	the bulk drugs	1673:1686	the bulk drugs	1673:1686	Finally, the validated method was applied for the determination and identification of cabotegravir and its chiral enantiomers in the bulk drugs by using HPLC and LC-MS techniques.					
36194910	2	76	from	drugs	509:513	arg1	year					543:546	the year 2021	539:551	the year 2021	539:551	Cabotegravir is an antiretroviral medication used for the treatment of HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021.					
36194910	5	77	theme	acid	1065:1068	arg1	addition					1046:1053	0.1% (v/v) addition	1035:1053	0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin-1	1035:1109	The mobile phase consisted of acetonitrile and water with 0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin-1.					
36194910	7	78	theme	calibration	1156:1166	arg1	linear					1216:1221	linear	1216:1221	linear	1216:1221	The calibration curves of cabotegravir and its enantiomers were linear over the concentration range of 0.04-1.125 µgmL-1.					
36194910	7	78	theme	calibration	1156:1166	arg1	curves					1168:1173	The calibration curves	1152:1173	The calibration curves of cabotegravir and its enantiomers	1152:1209	The calibration curves of cabotegravir and its enantiomers were linear over the concentration range of 0.04-1.125 µgmL-1.					
36194910	10	79	theme	validated	1557:1565	arg1	method					1567:1572	the validated method	1553:1572	the validated method	1553:1572	Finally, the validated method was applied for the determination and identification of cabotegravir and its chiral enantiomers in the bulk drugs by using HPLC and LC-MS techniques.					
36194910	8	80	theme	RS	1354:1355	arg1	enantiomer					1342:1351	its enantiomer	1338:1351	its enantiomer (RS isomer)	1338:1363	The limits of detection and quantification for cabotegravir and its enantiomer (RS isomer) were ≤ 0.02 and ≤ 0.06, and the RR and SS-isomers limits were ≤ 0.02 and 0.03 µgmL-1 respectively.					
36194910	8	80	theme	RS	1354:1355	arg1	isomer					1357:1362	RS isomer	1354:1362	RS isomer	1354:1362	The limits of detection and quantification for cabotegravir and its enantiomer (RS isomer) were ≤ 0.02 and ≤ 0.06, and the RR and SS-isomers limits were ≤ 0.02 and 0.03 µgmL-1 respectively.					
37299028	11	0	from	effects	1617:1623	arg1	cheese					1677:1682	cheese	1677:1682	cheese	1677:1682	In general, the results of this research showed that alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone had antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.					
37299028	9	1	theme	oils	1239:1242	arg1	effects					1191:1197	the effects	1187:1197	the effects of pulegone and M. longifolia essential oils on bacteria	1187:1254	When the effects of pulegone and M. longifolia essential oils on bacteria were compared, it was found that pulegone had a stronger effect on bacterial population reduction.					
37299028	4	2	theme	essential	642:650	arg1	oil					652:654	essential oil	642:654	essential oil	642:654	Plant samples were dried in the shade at ambient temperature, and essential oil was prepared using Clevenger.					
37299028	6	3	theme	oil	822:824	arg1	pulegone					830:837	pulegone	830:837	pulegone (26.07%)	830:846	The major composition of M. longifolia oil was pulegone (26.07%), piperitone oxide (19.72%), and piperitone (11.88%).					
37299028	6	3	theme	oil	822:824	arg1	composition					793:803	The major composition	783:803	The major composition of M. longifolia oil	783:824	The major composition of M. longifolia oil was pulegone (26.07%), piperitone oxide (19.72%), and piperitone (11.88%).					
37299028	1	4	theme	Mentha	139:144	arg1	plant					194:198	a valuable medicinal and aromatic plant	160:198	a valuable medicinal and aromatic plant that belongs to Lamiaceae family	160:231	Mentha longifolia is a valuable medicinal and aromatic plant that belongs to Lamiaceae family.					
37299028	1	4	theme	Mentha	139:144	arg1	longifolia					146:155	Mentha longifolia	139:155	Mentha longifolia	139:155	Mentha longifolia is a valuable medicinal and aromatic plant that belongs to Lamiaceae family.					
37299028	0	5	theme	Alginate	67:74	arg1	Coatings					55:62	Edible Coatings	48:62	Edible Coatings of Alginate and Chitosan	48:87	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	8	6	from	concentration	1107:1119	arg1	coatings					1172:1179	edible coatings	1165:1179	edible coatings	1165:1179	The bacterial population decreased by increasing the concentration of chitosan, M. longifolia, and pulegone in edible coatings.					
37299028	8	7	theme	chitosan	1124:1131	arg1	concentration					1107:1119	the concentration	1103:1119	the concentration of chitosan, M. longifolia, and pulegone in edible coatings	1103:1179	The bacterial population decreased by increasing the concentration of chitosan, M. longifolia, and pulegone in edible coatings.					
37299028	2	8	theme	edible	328:333	arg1	coatings					335:342	edible coatings	328:342	edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese	328:467	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	11	9	theme	ingredient	1579:1588	arg1	pulegone					1590:1597	its active ingredient pulegone	1568:1597	its active ingredient pulegone	1568:1597	In general, the results of this research showed that alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone had antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.					
37299028	5	10	dep	spectrometric	750:762	arg1	GC/MS					765:769	GC/MS	765:769	GC/MS	765:769	The essential oil was analyzed by gas chromatography using mass spectrometric (GC/MS) detection.					
37299028	11	11	theme	alginate	1494:1501	arg1	coatings					1516:1523	alginate and chitosan coatings	1494:1523	alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone	1494:1597	In general, the results of this research showed that alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone had antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.					
37299028	0	12	theme	Chitosan	80:87	arg1	Coatings					55:62	Edible Coatings	48:62	Edible Coatings of Alginate and Chitosan	48:87	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	2	13	theme	antibacterial	259:271	arg1	effects					273:279	the antibacterial effects	255:279	the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese	255:467	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	9	14	theme	stronger	1304:1311	arg1	effect					1313:1318	a stronger effect	1302:1318	a stronger effect	1302:1318	When the effects of pulegone and M. longifolia essential oils on bacteria were compared, it was found that pulegone had a stronger effect on bacterial population reduction.					
37299028	11	15	theme	M.	1536:1537	arg1	oil					1560:1562	M. longifolia essential oil	1536:1562	M. longifolia essential oil	1536:1562	In general, the results of this research showed that alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone had antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.					
37299028	9	16	theme	pulegone	1202:1209	arg1	effects					1191:1197	the effects	1187:1197	the effects of pulegone and M. longifolia essential oils on bacteria	1187:1254	When the effects of pulegone and M. longifolia essential oils on bacteria were compared, it was found that pulegone had a stronger effect on bacterial population reduction.					
37299028	7	17	theme	edible	977:982	arg1	coatings					984:991	edible coatings	977:991	edible coatings	977:991	The results showed that adding M. longifolia essential oils and pulegone to edible coatings significantly reduced the growth of bacteria during storage.					
37299028	5	18	theme	spectrometric	750:762	arg1	detection					772:780	mass spectrometric (GC/MS) detection	745:780	mass spectrometric (GC/MS) detection	745:780	The essential oil was analyzed by gas chromatography using mass spectrometric (GC/MS) detection.					
37299028	3	19	theme	fresh	494:498	arg1	plant					505:509	first fresh mint plant	488:509	first fresh mint plant	488:509	For this purpose, first fresh mint plant was collected from the cold region of Jiroft in Kerman province.					
37299028	4	20	theme	ambient	617:623	arg1	temperature					625:635	ambient temperature	617:635	ambient temperature	617:635	Plant samples were dried in the shade at ambient temperature, and essential oil was prepared using Clevenger.					
37299028	8	21	theme	longifolia	1137:1146	arg1	concentration					1107:1119	the concentration	1103:1119	the concentration of chitosan, M. longifolia, and pulegone in edible coatings	1103:1179	The bacterial population decreased by increasing the concentration of chitosan, M. longifolia, and pulegone in edible coatings.					
37299028	5	22	theme	essential	690:698	arg1	oil					700:702	The essential oil	686:702	The essential oil	686:702	The essential oil was analyzed by gas chromatography using mass spectrometric (GC/MS) detection.					
37299028	0	23	from	Pulegone	36:43	arg1	Coatings					55:62	Edible Coatings	48:62	Edible Coatings of Alginate and Chitosan	48:87	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	5	24	theme	gas	720:722	arg1	chromatography					724:737	gas chromatography	720:737	gas chromatography using mass spectrometric (GC/MS) detection	720:780	The essential oil was analyzed by gas chromatography using mass spectrometric (GC/MS) detection.					
37299028	0	25	theme	Mentha	0:5	arg1	Oil					28:30	Mentha longifolia Essential Oil	0:30	Mentha longifolia Essential Oil	0:30	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	0	26	theme	longifolia	7:16	arg1	Oil					28:30	Mentha longifolia Essential Oil	0:30	Mentha longifolia Essential Oil	0:30	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	3	27	theme	first	488:492	arg1	plant					505:509	first fresh mint plant	488:509	first fresh mint plant	488:509	For this purpose, first fresh mint plant was collected from the cold region of Jiroft in Kerman province.					
37299028	11	28	theme	chitosan	1507:1514	arg1	coatings					1516:1523	alginate and chitosan coatings	1494:1523	alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone	1494:1597	In general, the results of this research showed that alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone had antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.					
37299028	2	29	theme	essential	298:306	arg1	oil					308:310	M. longifolia essential oil	284:310	M. longifolia essential oil	284:310	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	1	30	theme	valuable	162:169	arg1	plant					194:198	a valuable medicinal and aromatic plant	160:198	a valuable medicinal and aromatic plant that belongs to Lamiaceae family	160:231	Mentha longifolia is a valuable medicinal and aromatic plant that belongs to Lamiaceae family.					
37299028	1	30	theme	valuable	162:169	arg1	longifolia					146:155	Mentha longifolia	139:155	Mentha longifolia	139:155	Mentha longifolia is a valuable medicinal and aromatic plant that belongs to Lamiaceae family.					
37299028	2	31	theme	Listeria	414:421	arg1	monocytogenes					423:435	Listeria monocytogenes	414:435	Listeria monocytogenes	414:435	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	9	32	theme	longifolia	1218:1227	arg1	oils					1239:1242	M. longifolia essential oils	1215:1242	M. longifolia essential oils	1215:1242	When the effects of pulegone and M. longifolia essential oils on bacteria were compared, it was found that pulegone had a stronger effect on bacterial population reduction.					
37299028	11	33	theme	antibacterial	1603:1615	arg1	effects					1617:1623	antibacterial effects	1603:1623	antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese	1603:1682	In general, the results of this research showed that alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone had antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.					
37299028	9	34	contain	had	1298:1300	arg2	effect					1313:1318	a stronger effect	1302:1318	a stronger effect	1302:1318	When the effects of pulegone and M. longifolia essential oils on bacteria were compared, it was found that pulegone had a stronger effect on bacterial population reduction.					
37299028	9	34	contain	had	1298:1300	arg1	pulegone					1289:1296	pulegone	1289:1296	pulegone	1289:1296	When the effects of pulegone and M. longifolia essential oils on bacteria were compared, it was found that pulegone had a stronger effect on bacterial population reduction.					
37299028	3	35	theme	cold	534:537	arg1	region					539:544	the cold region	530:544	the cold region of Jiroft	530:554	For this purpose, first fresh mint plant was collected from the cold region of Jiroft in Kerman province.					
37299028	8	36	theme	pulegone	1153:1160	arg1	concentration					1107:1119	the concentration	1103:1119	the concentration of chitosan, M. longifolia, and pulegone in edible coatings	1103:1179	The bacterial population decreased by increasing the concentration of chitosan, M. longifolia, and pulegone in edible coatings.					
37299028	1	37	theme	medicinal	171:179	arg1	plant					194:198	a valuable medicinal and aromatic plant	160:198	a valuable medicinal and aromatic plant that belongs to Lamiaceae family	160:231	Mentha longifolia is a valuable medicinal and aromatic plant that belongs to Lamiaceae family.					
37299028	1	37	theme	medicinal	171:179	arg1	longifolia					146:155	Mentha longifolia	139:155	Mentha longifolia	139:155	Mentha longifolia is a valuable medicinal and aromatic plant that belongs to Lamiaceae family.					
37299028	8	38	theme	bacterial	1058:1066	arg1	population					1068:1077	The bacterial population	1054:1077	The bacterial population	1054:1077	The bacterial population decreased by increasing the concentration of chitosan, M. longifolia, and pulegone in edible coatings.					
37299028	2	39	theme	M.	284:285	arg1	oil					308:310	M. longifolia essential oil	284:310	M. longifolia essential oil	284:310	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	9	40	theme	bacterial	1323:1331	arg1	reduction					1344:1352	bacterial population reduction	1323:1352	bacterial population reduction	1323:1352	When the effects of pulegone and M. longifolia essential oils on bacteria were compared, it was found that pulegone had a stronger effect on bacterial population reduction.					
37299028	0	41	theme	Essential	18:26	arg1	Oil					28:30	Mentha longifolia Essential Oil	0:30	Mentha longifolia Essential Oil	0:30	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	7	42	theme	longifolia	935:944	arg1	oils					956:959	M. longifolia essential oils	932:959	M. longifolia essential oils	932:959	The results showed that adding M. longifolia essential oils and pulegone to edible coatings significantly reduced the growth of bacteria during storage.					
37299028	0	43	theme	Pathogenic	101:110	arg1	Bacteria					112:119	Pathogenic Bacteria	101:119	Pathogenic Bacteria in Lactic Cheese	101:136	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	6	44	theme	longifolia	811:820	arg1	oil					822:824	M. longifolia oil	808:824	M. longifolia oil	808:824	The major composition of M. longifolia oil was pulegone (26.07%), piperitone oxide (19.72%), and piperitone (11.88%).					
37299028	1	45	theme	aromatic	185:192	arg1	plant					194:198	a valuable medicinal and aromatic plant	160:198	a valuable medicinal and aromatic plant that belongs to Lamiaceae family	160:231	Mentha longifolia is a valuable medicinal and aromatic plant that belongs to Lamiaceae family.					
37299028	1	45	theme	aromatic	185:192	arg1	longifolia					146:155	Mentha longifolia	139:155	Mentha longifolia	139:155	Mentha longifolia is a valuable medicinal and aromatic plant that belongs to Lamiaceae family.					
37299028	2	46	theme	coli	454:457	arg1	growth					381:386	the growth	377:386	the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese	377:467	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	11	47	dep	M.	1536:1537	arg1	longifolia					1539:1548	longifolia	1539:1548	longifolia	1539:1548	In general, the results of this research showed that alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone had antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.					
37299028	0	48	theme	Lactic	124:129	arg1	Cheese					131:136	Lactic Cheese	124:136	Lactic Cheese	124:136	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	6	49	theme	M.	808:809	arg1	oil					822:824	M. longifolia oil	808:824	M. longifolia oil	808:824	The major composition of M. longifolia oil was pulegone (26.07%), piperitone oxide (19.72%), and piperitone (11.88%).					
37299028	3	50	theme	Jiroft	549:554	arg1	region					539:544	the cold region	530:544	the cold region of Jiroft	530:554	For this purpose, first fresh mint plant was collected from the cold region of Jiroft in Kerman province.					
37299028	9	51	theme	population	1333:1342	arg1	reduction					1344:1352	bacterial population reduction	1323:1352	bacterial population reduction	1323:1352	When the effects of pulegone and M. longifolia essential oils on bacteria were compared, it was found that pulegone had a stronger effect on bacterial population reduction.					
37299028	9	52	from	effects	1191:1197	arg1	bacteria					1247:1254	bacteria	1247:1254	bacteria	1247:1254	When the effects of pulegone and M. longifolia essential oils on bacteria were compared, it was found that pulegone had a stronger effect on bacterial population reduction.					
37299028	7	53	theme	essential	946:954	arg1	oils					956:959	M. longifolia essential oils	932:959	M. longifolia essential oils	932:959	The results showed that adding M. longifolia essential oils and pulegone to edible coatings significantly reduced the growth of bacteria during storage.					
37299028	0	54	from	Bacteria	112:119	arg1	Cheese					131:136	Lactic Cheese	124:136	Lactic Cheese	124:136	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	11	55	theme	research	1473:1480	arg1	results					1457:1463	the results	1453:1463	the results of this research	1453:1480	In general, the results of this research showed that alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone had antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.					
37299028	8	56	theme	edible	1165:1170	arg1	coatings					1172:1179	edible coatings	1165:1179	edible coatings	1165:1179	The bacterial population decreased by increasing the concentration of chitosan, M. longifolia, and pulegone in edible coatings.					
37299028	0	57	theme	Edible	48:53	arg1	Coatings					55:62	Edible Coatings	48:62	Edible Coatings of Alginate and Chitosan	48:87	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	9	58	theme	essential	1229:1237	arg1	oils					1239:1242	M. longifolia essential oils	1215:1242	M. longifolia essential oils	1215:1242	When the effects of pulegone and M. longifolia essential oils on bacteria were compared, it was found that pulegone had a stronger effect on bacterial population reduction.					
37299028	2	59	dep	M.	284:285	arg1	longifolia					287:296	longifolia	287:296	longifolia	287:296	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	7	60	theme	bacteria	1029:1036	arg1	growth					1019:1024	the growth	1015:1024	the growth of bacteria	1015:1036	The results showed that adding M. longifolia essential oils and pulegone to edible coatings significantly reduced the growth of bacteria during storage.					
37299028	7	61	theme	M.	932:933	arg1	oils					956:959	M. longifolia essential oils	932:959	M. longifolia essential oils	932:959	The results showed that adding M. longifolia essential oils and pulegone to edible coatings significantly reduced the growth of bacteria during storage.					
37299028	6	62	theme	major	787:791	arg1	pulegone					830:837	pulegone	830:837	pulegone (26.07%)	830:846	The major composition of M. longifolia oil was pulegone (26.07%), piperitone oxide (19.72%), and piperitone (11.88%).					
37299028	6	62	theme	major	787:791	arg1	composition					793:803	The major composition	783:803	The major composition of M. longifolia oil	783:824	The major composition of M. longifolia oil was pulegone (26.07%), piperitone oxide (19.72%), and piperitone (11.88%).					
37299028	10	63	theme	Coating	1355:1361	arg1	treatments					1363:1372	Coating treatments	1355:1372	Coating treatments	1355:1372	Coating treatments showed more antibacterial activity on E. coli than other bacteria.					
37299028	2	64	theme	pulegone	316:323	arg1	effects					273:279	the antibacterial effects	255:279	the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese	255:467	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	6	65	theme	piperitone	849:858	arg1	oxide					860:864	piperitone oxide	849:864	piperitone oxide (19.72%)	849:873	The major composition of M. longifolia oil was pulegone (26.07%), piperitone oxide (19.72%), and piperitone (11.88%).					
37299028	6	65	theme	piperitone	849:858	arg1	%					872:872	19.72%	867:872	19.72%	867:872	The major composition of M. longifolia oil was pulegone (26.07%), piperitone oxide (19.72%), and piperitone (11.88%).					
37299028	0	66	from	Effects	90:96	arg1	Bacteria					112:119	Pathogenic Bacteria	101:119	Pathogenic Bacteria in Lactic Cheese	101:136	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	0	67	dep	Oil	28:30	arg1	Effects					90:96	Effects	90:96	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.	0:137	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	11	68	contain	had	1599:1601	arg1	coatings					1516:1523	alginate and chitosan coatings	1494:1523	alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone	1494:1597	In general, the results of this research showed that alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone had antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.					
37299028	11	68	contain	had	1599:1601	arg2	effects					1617:1623	antibacterial effects	1603:1623	antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese	1603:1682	In general, the results of this research showed that alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone had antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.					
37299028	3	69	theme	mint	500:503	arg1	plant					505:509	first fresh mint plant	488:509	first fresh mint plant	488:509	For this purpose, first fresh mint plant was collected from the cold region of Jiroft in Kerman province.					
37299028	2	70	from	growth	381:386	arg1	cheese					462:467	cheese	462:467	cheese	462:467	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	2	71	theme	oil	308:310	arg1	effects					273:279	the antibacterial effects	255:279	the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese	255:467	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	2	72	from	effects	273:279	arg1	coatings					335:342	edible coatings	328:342	edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese	328:467	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	10	73	theme	antibacterial	1386:1398	arg1	activity					1400:1407	more antibacterial activity	1381:1407	more antibacterial activity	1381:1407	Coating treatments showed more antibacterial activity on E. coli than other bacteria.					
37299028	9	74	theme	M.	1215:1216	arg1	oils					1239:1242	M. longifolia essential oils	1215:1242	M. longifolia essential oils	1215:1242	When the effects of pulegone and M. longifolia essential oils on bacteria were compared, it was found that pulegone had a stronger effect on bacterial population reduction.					
37299028	3	75	theme	Kerman	559:564	arg1	province					566:573	Kerman province	559:573	Kerman province	559:573	For this purpose, first fresh mint plant was collected from the cold region of Jiroft in Kerman province.					
37299028	4	76	theme	Plant	576:580	arg1	samples					582:588	Plant samples	576:588	Plant samples	576:588	Plant samples were dried in the shade at ambient temperature, and essential oil was prepared using Clevenger.					
37299028	10	77	theme	other	1425:1429	arg1	bacteria					1431:1438	other bacteria	1425:1438	other bacteria	1425:1438	Coating treatments showed more antibacterial activity on E. coli than other bacteria.					
37299028	1	78	theme	Lamiaceae	216:224	arg1	family					226:231	Lamiaceae family	216:231	Lamiaceae family	216:231	Mentha longifolia is a valuable medicinal and aromatic plant that belongs to Lamiaceae family.					
37299028	0	79	from	Oil	28:30	arg1	Coatings					55:62	Edible Coatings	48:62	Edible Coatings of Alginate and Chitosan	48:87	Mentha longifolia Essential Oil and Pulegone in Edible Coatings of Alginate and Chitosan: Effects on Pathogenic Bacteria in Lactic Cheese.					
37299028	2	80	theme	aureus	406:411	arg1	growth					381:386	the growth	377:386	the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese	377:467	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	2	81	theme	monocytogenes	423:435	arg1	growth					381:386	the growth	377:386	the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese	377:467	This study looked at the antibacterial effects of M. longifolia essential oil and pulegone in edible coatings made of chitosan and alginate on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese.					
37299028	11	82	theme	active	1572:1577	arg1	ingredient					1579:1588	its active ingredient	1568:1588	its active ingredient pulegone	1568:1597	In general, the results of this research showed that alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone had antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.					
37299028	11	83	theme	essential	1550:1558	arg1	oil					1560:1562	M. longifolia essential oil	1536:1562	M. longifolia essential oil	1536:1562	In general, the results of this research showed that alginate and chitosan coatings along with M. longifolia essential oil and its active ingredient pulegone had antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.					
36516686	4	0	theme	neurotrophic	1105:1116	arg1	GDNF					1126:1129	GDNF	1126:1129	GDNF	1126:1129	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	0	theme	neurotrophic	1105:1116	arg1	factor					1118:1123	bioactive glial cell-derived neurotrophic factor	1076:1123	bioactive glial cell-derived neurotrophic factor (GDNF)	1076:1130	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	1	with	ECT	794:796	arg1	poly-l-lysine					892:904	0.01% or 0.05% poly-l-lysine	877:904	0.01% or 0.05% poly-l-lysine coating for 5 min	877:922	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	2	2	theme	viability	440:448	arg1	issues					490:495	face various biocompatibility, viability, drug delivery and mechanical stability issues	409:495	face various biocompatibility, viability, drug delivery and mechanical stability issues	409:495	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	8	3	theme	delivery	1728:1735	arg1	platforms					1737:1745	well-controlled, sustained drug delivery platforms	1696:1745	well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression	1696:1807	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	8	3	theme	delivery	1728:1735	arg1	gels					1678:1681	both coated and non-coated ECT gels	1647:1681	both coated and non-coated ECT gels	1647:1681	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	2	4	theme	in	377:378	arg1	delivery					390:397	prolonged in vivo drug delivery	367:397	prolonged in vivo drug delivery	367:397	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	1	5	theme	delivery	227:234	arg1	difficulties					236:247	drug delivery difficulties	222:247	drug delivery difficulties	222:247	Treatments of vision-threatening retinal diseases are often hampered by drug delivery difficulties.					
36516686	4	6	theme	rat	1184:1186	arg1	vitreous					1188:1195	rat vitreous	1184:1195	rat vitreous	1184:1195	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	7	theme	AP-coated	759:767	arg1	ECT					794:796	AP5.5-CAC ECT	784:796	AP5.5-CAC ECT	784:796	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	7	theme	AP-coated	759:767	arg1	gels					769:772	All optimised AP-coated gels	745:772	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating)	745:959	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	7	theme	AP-coated	759:767	arg1	alginate					844:851	1.5% high molecular weight alginate	817:851	1.5% high molecular weight alginate	817:851	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	7	theme	AP-coated	759:767	arg1	AP1-					775:778	AP1-	775:778	AP1-	775:778	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	7	theme	AP-coated	759:767	arg1	collagen					807:814	2 mg/ml collagen	799:814	2 mg/ml collagen	799:814	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	7	theme	AP-coated	759:767	arg1	50,000 cells/gel					854:869	50,000 cells/gel	854:869	50,000 cells/gel	854:869	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	8	with	alginate	844:851	arg1	poly-l-lysine					892:904	0.01% or 0.05% poly-l-lysine	877:904	0.01% or 0.05% poly-l-lysine coating for 5 min	877:922	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	9	theme	gel	1152:1154	arg1	degradation					1156:1166	no significant gel degradation	1137:1166	no significant gel degradation in vitro and in rat vitreous	1137:1195	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	3	10	theme	ocular	648:653	arg1	delivery					660:667	sustained ocular drug delivery	638:667	sustained ocular drug delivery	638:667	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	5	11	from	efficacy	1270:1277	arg1	College					1288:1294	College	1288:1294	College	1288:1294	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with retinal degeneration, resulting in reduced photoreceptor apoptosis and retinal function loss.					
36516686	4	12	dep	poly-l-lysine	892:904	arg1	%					890:890	%	890:890	%	890:890	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	13	theme	%	941:941	arg1	coating					952:958	0.15% alginate coating	937:958	0.15% alginate coating	937:958	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	1	14	theme	vision-threatening	164:181	arg1	diseases					191:198	vision-threatening retinal diseases	164:198	vision-threatening retinal diseases	164:198	Treatments of vision-threatening retinal diseases are often hampered by drug delivery difficulties.					
36516686	4	15	theme	2 mg/ml	799:805	arg1	collagen					807:814	2 mg/ml collagen	799:814	2 mg/ml collagen	799:814	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	15	theme	2 mg/ml	799:805	arg1	gels					769:772	All optimised AP-coated gels	745:772	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating)	745:959	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	2	16	theme	therapeutic	338:348	arg1	efficacy					350:357	therapeutic efficacy	338:357	therapeutic efficacy	338:357	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	1	17	theme	diseases	191:198	arg1	Treatments					150:159	Treatments	150:159	Treatments of vision-threatening retinal diseases	150:198	Treatments of vision-threatening retinal diseases are often hampered by drug delivery difficulties.					
36516686	5	18	theme	photoreceptor	1361:1373	arg1	apoptosis					1375:1383	reduced photoreceptor apoptosis	1353:1383	reduced photoreceptor apoptosis	1353:1383	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with retinal degeneration, resulting in reduced photoreceptor apoptosis and retinal function loss.					
36516686	6	19	theme	retrieved	1501:1509	arg1	gels					1511:1514	the retrieved gels	1497:1514	the retrieved gels	1497:1514	At 6 months post-implantation, no host-tissue attachment or ingrowth was detected on the retrieved gels.					
36516686	4	20	theme	viability	1031:1039	arg1	support					1041:1047	cell viability support	1026:1047	cell viability support	1026:1047	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	21	link	cell-derived	1092:1103	arg1	GDNF					1126:1129	GDNF	1126:1129	GDNF	1126:1129	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	21	link	cell-derived	1092:1103	arg1	factor					1118:1123	bioactive glial cell-derived neurotrophic factor	1076:1123	bioactive glial cell-derived neurotrophic factor (GDNF)	1076:1130	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	5	22	theme	retinal	1318:1324	arg1	degeneration					1326:1337	retinal degeneration	1318:1337	retinal degeneration	1318:1337	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with retinal degeneration, resulting in reduced photoreceptor apoptosis and retinal function loss.					
36516686	6	23	theme	host-tissue	1446:1456	arg1	attachment					1458:1467	no host-tissue attachment	1443:1467	no host-tissue attachment	1443:1467	At 6 months post-implantation, no host-tissue attachment or ingrowth was detected on the retrieved gels.					
36516686	4	24	theme	effective	988:996	arg1	control					1017:1023	effective cell proliferation control	988:1023	effective cell proliferation control	988:1023	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	25	theme	molecular	827:835	arg1	gels					769:772	All optimised AP-coated gels	745:772	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating)	745:959	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	25	theme	molecular	827:835	arg1	alginate					844:851	1.5% high molecular weight alginate	817:851	1.5% high molecular weight alginate	817:851	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	26	with	50,000 cells/gel	854:869	arg1	poly-l-lysine					892:904	0.01% or 0.05% poly-l-lysine	877:904	0.01% or 0.05% poly-l-lysine coating for 5 min	877:922	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	0	27	theme	retinal	128:134	arg1	degeneration					136:147	retinal degeneration	128:147	retinal degeneration	128:147	Both non-coated and polyelectrolytically-coated intraocular collagen-alginate composite gels enhanced photoreceptor survival in retinal degeneration.					
36516686	8	28	dep	well-controlled	1696:1710	arg1	sustained					1713:1721	sustained	1713:1721	sustained	1713:1721	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	4	29	theme	proliferation	1003:1015	arg1	control					1017:1023	effective cell proliferation control	988:1023	effective cell proliferation control	988:1023	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	3	30	theme	novel	523:527	arg1	CAC					605:607	CAC	605:607	CAC	605:607	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	3	30	theme	novel	523:527	arg1	alginate-collagen					586:602	novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen	523:602	novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels	523:617	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	4	31	dep	gels	769:772	arg1	ECT					794:796	AP5.5-CAC ECT	784:796	AP5.5-CAC ECT	784:796	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	31	dep	gels	769:772	arg1	gels					769:772	All optimised AP-coated gels	745:772	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating)	745:959	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	31	dep	gels	769:772	arg1	alginate					844:851	1.5% high molecular weight alginate	817:851	1.5% high molecular weight alginate	817:851	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	31	dep	gels	769:772	arg1	AP1-					775:778	AP1-	775:778	AP1-	775:778	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	31	dep	gels	769:772	arg1	collagen					807:814	2 mg/ml collagen	799:814	2 mg/ml collagen	799:814	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	31	dep	gels	769:772	arg1	50,000 cells/gel					854:869	50,000 cells/gel	854:869	50,000 cells/gel	854:869	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	32	theme	bioactive	1076:1084	arg1	GDNF					1126:1129	GDNF	1126:1129	GDNF	1126:1129	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	32	theme	bioactive	1076:1084	arg1	factor					1118:1123	bioactive glial cell-derived neurotrophic factor	1076:1123	bioactive glial cell-derived neurotrophic factor (GDNF)	1076:1130	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	2	33	theme	mechanical	469:478	arg1	stability					480:488	mechanical stability	469:488	mechanical stability	469:488	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	4	34	theme	cell-derived	1092:1103	arg1	GDNF					1126:1129	GDNF	1126:1129	GDNF	1126:1129	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	34	theme	cell-derived	1092:1103	arg1	factor					1118:1123	bioactive glial cell-derived neurotrophic factor	1076:1123	bioactive glial cell-derived neurotrophic factor (GDNF)	1076:1130	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	2	35	theme	drug	451:454	arg1	delivery					456:463	drug delivery	451:463	drug delivery	451:463	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	4	36	theme	%	820:820	arg1	gels					769:772	All optimised AP-coated gels	745:772	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating)	745:959	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	36	theme	%	820:820	arg1	alginate					844:851	1.5% high molecular weight alginate	817:851	1.5% high molecular weight alginate	817:851	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	37	dep	%	890:890	arg1	%					881:881	%	881:881	%	881:881	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	37	dep	%	890:890	arg1	0.05					886:889	0.05	886:889	0.05	886:889	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	5	38	theme	function	1397:1404	arg1	loss					1406:1409	retinal function loss	1389:1409	retinal function loss	1389:1409	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with retinal degeneration, resulting in reduced photoreceptor apoptosis and retinal function loss.					
36516686	2	39	theme	clinical	500:507	arg1	trials					509:514	clinical trials	500:514	clinical trials	500:514	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	0	40	theme	intraocular	48:58	arg1	collagen-alginate					60:76	polyelectrolytically-coated intraocular collagen-alginate	20:76	polyelectrolytically-coated intraocular collagen-alginate	20:76	Both non-coated and polyelectrolytically-coated intraocular collagen-alginate composite gels enhanced photoreceptor survival in retinal degeneration.					
36516686	3	41	theme	CAC	731:733	arg1	gels					739:742	non-coated CAC ECT gels	720:742	non-coated CAC ECT gels	720:742	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	3	42	theme	composite	576:584	arg1	CAC					605:607	CAC	605:607	CAC	605:607	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	3	42	theme	composite	576:584	arg1	alginate-collagen					586:602	novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen	523:602	novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels	523:617	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	0	43	theme	composite	78:86	arg1	gels					88:91	composite gels	78:91	composite gels	78:91	Both non-coated and polyelectrolytically-coated intraocular collagen-alginate composite gels enhanced photoreceptor survival in retinal degeneration.					
36516686	7	44	theme	current	1596:1602	arg1	loading					1609:1615	the current cell loading	1592:1615	the current cell loading	1592:1615	Non-coated gels were mechanically more stable than AP5.5-coated ones under the current cell loading.					
36516686	8	45	theme	ECT	1674:1676	arg1	platforms					1737:1745	well-controlled, sustained drug delivery platforms	1696:1745	well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression	1696:1807	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	8	45	theme	ECT	1674:1676	arg1	gels					1678:1681	both coated and non-coated ECT gels	1647:1681	both coated and non-coated ECT gels	1647:1681	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	2	46	theme	encapsulated-cell	287:303	arg1	ECT					314:316	ECT	314:316	ECT	314:316	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	2	46	theme	encapsulated-cell	287:303	arg1	therapy					305:311	alginate encapsulated-cell therapy	278:311	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems	250:325	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	2	47	theme	various	414:420	arg1	biocompatibility					422:437	face various biocompatibility	409:437	face various biocompatibility	409:437	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	4	48	theme	significant	1140:1150	arg1	degradation					1156:1166	no significant gel degradation	1137:1166	no significant gel degradation in vitro and in rat vitreous	1137:1195	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	2	49	theme	Polyelectrolytically-coated	250:276	arg1	systems					319:325	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems	250:325	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems	250:325	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	5	50	with	rats	1308:1311	arg1	degeneration					1326:1337	retinal degeneration	1318:1337	retinal degeneration	1318:1337	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with retinal degeneration, resulting in reduced photoreceptor apoptosis and retinal function loss.					
36516686	2	51	theme	delivery	456:463	arg1	issues					490:495	face various biocompatibility, viability, drug delivery and mechanical stability issues	409:495	face various biocompatibility, viability, drug delivery and mechanical stability issues	409:495	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	5	52	theme	Surgeons	1299:1306	arg1	rats					1308:1311	Surgeons rats	1299:1311	Surgeons rats with retinal degeneration	1299:1337	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with retinal degeneration, resulting in reduced photoreceptor apoptosis and retinal function loss.					
36516686	7	53	theme	AP5.5-coated	1568:1579	arg1	ones					1581:1584	AP5.5-coated ones	1568:1584	AP5.5-coated ones	1568:1584	Non-coated gels were mechanically more stable than AP5.5-coated ones under the current cell loading.					
36516686	4	54	theme	factor	1118:1123	arg1	support					1041:1047	cell viability support	1026:1047	cell viability support	1026:1047	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	54	theme	factor	1118:1123	arg1	delivery					1064:1071	continuous delivery	1053:1071	continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF)	1053:1130	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	54	theme	factor	1118:1123	arg1	control					1017:1023	effective cell proliferation control	988:1023	effective cell proliferation control	988:1023	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	8	55	theme	eye	1770:1772	arg1	diseases					1774:1781	posterior eye diseases	1760:1781	posterior eye diseases	1760:1781	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	5	56	theme	therapeutic	1258:1268	arg1	efficacy					1270:1277	therapeutic efficacy	1258:1277	therapeutic efficacy in Royal College of Surgeons rats with retinal degeneration	1258:1337	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with retinal degeneration, resulting in reduced photoreceptor apoptosis and retinal function loss.					
36516686	7	57	theme	Non-coated	1517:1526	arg1	gels					1528:1531	Non-coated gels	1517:1531	Non-coated gels	1517:1531	Non-coated gels were mechanically more stable than AP5.5-coated ones under the current cell loading.					
36516686	8	58	theme	drug	1723:1726	arg1	platforms					1737:1745	well-controlled, sustained drug delivery platforms	1696:1745	well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression	1696:1807	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	8	58	theme	drug	1723:1726	arg1	gels					1678:1681	both coated and non-coated ECT gels	1647:1681	both coated and non-coated ECT gels	1647:1681	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	4	59	with	AP1-	775:778	arg1	poly-l-lysine					892:904	0.01% or 0.05% poly-l-lysine	877:904	0.01% or 0.05% poly-l-lysine coating for 5 min	877:922	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	2	60	theme	prolonged	367:375	arg1	delivery					390:397	prolonged in vivo drug delivery	367:397	prolonged in vivo drug delivery	367:397	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	1	61	theme	drug	222:225	arg1	difficulties					236:247	drug delivery difficulties	222:247	drug delivery difficulties	222:247	Treatments of vision-threatening retinal diseases are often hampered by drug delivery difficulties.					
36516686	3	62	theme	drug	655:658	arg1	delivery					660:667	sustained ocular drug delivery	638:667	sustained ocular drug delivery	638:667	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	4	63	theme	optimised	749:757	arg1	ECT					794:796	AP5.5-CAC ECT	784:796	AP5.5-CAC ECT	784:796	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	63	theme	optimised	749:757	arg1	gels					769:772	All optimised AP-coated gels	745:772	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating)	745:959	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	63	theme	optimised	749:757	arg1	alginate					844:851	1.5% high molecular weight alginate	817:851	1.5% high molecular weight alginate	817:851	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	63	theme	optimised	749:757	arg1	AP1-					775:778	AP1-	775:778	AP1-	775:778	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	63	theme	optimised	749:757	arg1	collagen					807:814	2 mg/ml collagen	799:814	2 mg/ml collagen	799:814	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	63	theme	optimised	749:757	arg1	50,000 cells/gel					854:869	50,000 cells/gel	854:869	50,000 cells/gel	854:869	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	3	64	theme	sustained	638:646	arg1	delivery					660:667	sustained ocular drug delivery	638:667	sustained ocular drug delivery	638:667	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	3	65	theme	alginate-collagen	586:602	arg1	gels					614:617	novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels	523:617	novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels	523:617	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	4	66	theme	non-coated	965:974	arg1	gels					976:979	non-coated gels	965:979	non-coated gels	965:979	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	67	theme	0.15	937:940	arg1	%					941:941	%	941:941	%	941:941	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	68	theme	AP5.5-CAC	784:792	arg1	ECT					794:796	AP5.5-CAC ECT	784:796	AP5.5-CAC ECT	784:796	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	68	theme	AP5.5-CAC	784:792	arg1	gels					769:772	All optimised AP-coated gels	745:772	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating)	745:959	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	69	with	collagen	807:814	arg1	poly-l-lysine					892:904	0.01% or 0.05% poly-l-lysine	877:904	0.01% or 0.05% poly-l-lysine coating for 5 min	877:922	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	6	70	located	detected	1485:1492	arg2	ingrowth					1472:1479	ingrowth	1472:1479	ingrowth	1472:1479	At 6 months post-implantation, no host-tissue attachment or ingrowth was detected on the retrieved gels.					
36516686	6	70	located	detected	1485:1492	arg1	gels					1511:1514	the retrieved gels	1497:1514	the retrieved gels	1497:1514	At 6 months post-implantation, no host-tissue attachment or ingrowth was detected on the retrieved gels.					
36516686	6	70	located	detected	1485:1492	arg1	months					1417:1422	6 months	1415:1422	6 months	1415:1422	At 6 months post-implantation, no host-tissue attachment or ingrowth was detected on the retrieved gels.					
36516686	6	70	located	detected	1485:1492	arg2	attachment					1458:1467	no host-tissue attachment	1443:1467	no host-tissue attachment	1443:1467	At 6 months post-implantation, no host-tissue attachment or ingrowth was detected on the retrieved gels.					
36516686	6	70	located	detected	1485:1492	arg2	post-implantation					1424:1440	post-implantation	1424:1440	post-implantation	1424:1440	At 6 months post-implantation, no host-tissue attachment or ingrowth was detected on the retrieved gels.					
36516686	1	71	theme	retinal	183:189	arg1	diseases					191:198	vision-threatening retinal diseases	164:198	vision-threatening retinal diseases	164:198	Treatments of vision-threatening retinal diseases are often hampered by drug delivery difficulties.					
36516686	4	72	theme	alginate	943:950	arg1	coating					952:958	0.15% alginate coating	937:958	0.15% alginate coating	937:958	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	5	73	theme	reduced	1353:1359	arg1	apoptosis					1375:1383	reduced photoreceptor apoptosis	1353:1383	reduced photoreceptor apoptosis	1353:1383	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with retinal degeneration, resulting in reduced photoreceptor apoptosis and retinal function loss.					
36516686	0	74	theme	photoreceptor	102:114	arg1	survival					116:123	photoreceptor survival	102:123	photoreceptor survival	102:123	Both non-coated and polyelectrolytically-coated intraocular collagen-alginate composite gels enhanced photoreceptor survival in retinal degeneration.					
36516686	4	75	theme	cell	1026:1029	arg1	support					1041:1047	cell viability support	1026:1047	cell viability support	1026:1047	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	2	76	dep	in	377:378	arg1	vivo					380:383	vivo	380:383	vivo	380:383	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	2	77	theme	drug	385:388	arg1	delivery					390:397	prolonged in vivo drug delivery	367:397	prolonged in vivo drug delivery	367:397	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	4	78	theme	high	822:825	arg1	gels					769:772	All optimised AP-coated gels	745:772	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating)	745:959	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	78	theme	high	822:825	arg1	alginate					844:851	1.5% high molecular weight alginate	817:851	1.5% high molecular weight alginate	817:851	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	2	79	theme	face	409:412	arg1	biocompatibility					422:437	face various biocompatibility	409:437	face various biocompatibility	409:437	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	4	80	theme	cell	998:1001	arg1	control					1017:1023	effective cell proliferation control	988:1023	effective cell proliferation control	988:1023	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	81	theme	weight	837:842	arg1	gels					769:772	All optimised AP-coated gels	745:772	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating)	745:959	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	81	theme	weight	837:842	arg1	alginate					844:851	1.5% high molecular weight alginate	817:851	1.5% high molecular weight alginate	817:851	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	2	82	theme	stability	480:488	arg1	issues					490:495	face various biocompatibility, viability, drug delivery and mechanical stability issues	409:495	face various biocompatibility, viability, drug delivery and mechanical stability issues	409:495	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	0	83	theme	polyelectrolytically-coated	20:46	arg1	collagen-alginate					60:76	polyelectrolytically-coated intraocular collagen-alginate	20:76	polyelectrolytically-coated intraocular collagen-alginate	20:76	Both non-coated and polyelectrolytically-coated intraocular collagen-alginate composite gels enhanced photoreceptor survival in retinal degeneration.					
36516686	5	84	theme	injected	1231:1238	arg1	gels					1240:1243	intravitreally injected gels	1216:1243	intravitreally injected gels	1216:1243	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with retinal degeneration, resulting in reduced photoreceptor apoptosis and retinal function loss.					
36516686	4	85	theme	glial	1086:1090	arg1	GDNF					1126:1129	GDNF	1126:1129	GDNF	1126:1129	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	4	85	theme	glial	1086:1090	arg1	factor					1118:1123	bioactive glial cell-derived neurotrophic factor	1076:1123	bioactive glial cell-derived neurotrophic factor (GDNF)	1076:1130	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	8	86	theme	coated	1652:1657	arg1	platforms					1737:1745	well-controlled, sustained drug delivery platforms	1696:1745	well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression	1696:1807	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	8	86	theme	coated	1652:1657	arg1	gels					1678:1681	both coated and non-coated ECT gels	1647:1681	both coated and non-coated ECT gels	1647:1681	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	3	87	theme	ECT	735:737	arg1	gels					739:742	non-coated CAC ECT gels	720:742	non-coated CAC ECT gels	720:742	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	4	88	theme	1.5	817:819	arg1	%					820:820	%	820:820	%	820:820	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	5	89	theme	retinal	1389:1395	arg1	loss					1406:1409	retinal function loss	1389:1409	retinal function loss	1389:1409	Most importantly, intravitreally injected gels demonstrated therapeutic efficacy in Royal College of Surgeons rats with retinal degeneration, resulting in reduced photoreceptor apoptosis and retinal function loss.					
36516686	8	90	theme	non-coated	1663:1672	arg1	platforms					1737:1745	well-controlled, sustained drug delivery platforms	1696:1745	well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression	1696:1807	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	8	90	theme	non-coated	1663:1672	arg1	gels					1678:1681	both coated and non-coated ECT gels	1647:1681	both coated and non-coated ECT gels	1647:1681	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	3	91	theme	non-coated	720:729	arg1	gels					739:742	non-coated CAC ECT gels	720:742	non-coated CAC ECT gels	720:742	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	3	92	dep	novel	523:527	arg1	injectable					530:539	injectable	530:539	injectable	530:539	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	4	93	theme	continuous	1053:1062	arg1	delivery					1064:1071	continuous delivery	1053:1071	continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF)	1053:1130	All optimised AP-coated gels (AP1- and AP5.5-CAC ECT: 2 mg/ml collagen, 1.5% high molecular weight alginate, 50,000 cells/gel, with 0.01% or 0.05% poly-l-lysine coating for 5 min, followed by 0.15% alginate coating) and non-coated gels showed effective cell proliferation control, cell viability support and continuous delivery of bioactive glial cell-derived neurotrophic factor (GDNF) with no significant gel degradation in vitro and in rat vitreous.					
36516686	2	94	theme	therapy	305:311	arg1	systems					319:325	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems	250:325	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems	250:325	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	2	95	theme	biocompatibility	422:437	arg1	issues					490:495	face various biocompatibility, viability, drug delivery and mechanical stability issues	409:495	face various biocompatibility, viability, drug delivery and mechanical stability issues	409:495	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	7	96	theme	cell	1604:1607	arg1	loading					1609:1615	the current cell loading	1592:1615	the current cell loading	1592:1615	Non-coated gels were mechanically more stable than AP5.5-coated ones under the current cell loading.					
36516686	3	97	theme	long-term	680:688	arg1	performance					690:700	their long-term performance	674:700	their long-term performance	674:700	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	2	98	theme	alginate	278:285	arg1	ECT					314:316	ECT	314:316	ECT	314:316	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	2	98	theme	alginate	278:285	arg1	therapy					305:311	alginate encapsulated-cell therapy	278:311	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems	250:325	Polyelectrolytically-coated alginate encapsulated-cell therapy (ECT) systems have shown therapeutic efficacy through prolonged in vivo drug delivery but still face various biocompatibility, viability, drug delivery and mechanical stability issues in clinical trials.					
36516686	3	99	theme	-coated	568:574	arg1	CAC					605:607	CAC	605:607	CAC	605:607	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	3	99	theme	-coated	568:574	arg1	alginate-collagen					586:602	novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen	523:602	novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels	523:617	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36516686	8	100	theme	posterior	1760:1768	arg1	diseases					1774:1781	posterior eye diseases	1760:1781	posterior eye diseases	1760:1781	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	0	101	dep	non-coated	5:14	arg1	gels					88:91	composite gels	78:91	composite gels	78:91	Both non-coated and polyelectrolytically-coated intraocular collagen-alginate composite gels enhanced photoreceptor survival in retinal degeneration.					
36516686	8	102	theme	well-controlled	1696:1710	arg1	platforms					1737:1745	well-controlled, sustained drug delivery platforms	1696:1745	well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression	1696:1807	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	8	102	theme	well-controlled	1696:1710	arg1	gels					1678:1681	both coated and non-coated ECT gels	1647:1681	both coated and non-coated ECT gels	1647:1681	This study demonstrated that both coated and non-coated ECT gels can serve as well-controlled, sustained drug delivery platforms for treating posterior eye diseases without immunosuppression.					
36516686	3	103	theme	ECT	610:612	arg1	gels					614:617	novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels	523:617	novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels	523:617	Here, novel, injectable alginate-poly-l-lysine (AP)-coated composite alginate-collagen (CAC) ECT gels were developed for sustained ocular drug delivery, and their long-term performance was compared with non-coated CAC ECT gels.					
36498931	0	0	theme	Cell	96:99	arg1	Behavior					101:108	Endothelial Cell Behavior	84:108	Endothelial Cell Behavior	84:108	Spatial-Controlled Coating of Pro-Angiogenic Proteins on 3D Porous Hydrogels Guides Endothelial Cell Behavior.					
36498931	5	1	theme	preformed	791:799	arg1	microchannels					801:813	preformed microchannels	791:813	preformed microchannels	791:813	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	4	2	theme	endothelial	734:744	arg1	EC					752:753	EC	752:753	EC	752:753	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36498931	4	2	theme	endothelial	734:744	arg1	cell					746:749	endothelial cell	734:749	endothelial cell (EC) behavior	734:763	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36498931	10	3	theme	cell	1479:1482	arg1	migration					1484:1492	directed cell migration	1470:1492	directed cell migration	1470:1492	Most importantly, directed cell migration required the presence of both proteins in the microchannel and in the pores, highlighting the need for an adhesive substrate provided by Caf1-YIGSR for Caf1-VEGF to be effective.					
36498931	1	4	theme	structural	158:167	arg1	arrangement					169:179	the structural arrangement	154:179	the structural arrangement	154:179	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	1	5	theme	physical	257:264	arg1	features					282:289	the physical and biochemical features	253:289	the physical and biochemical features of a scaffold	253:303	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	0	6	theme	Endothelial	84:94	arg1	Behavior					101:108	Endothelial Cell Behavior	84:108	Endothelial Cell Behavior	84:108	Spatial-Controlled Coating of Pro-Angiogenic Proteins on 3D Porous Hydrogels Guides Endothelial Cell Behavior.					
36498931	0	7	from	Coating	19:25	arg1	Hydrogels					67:75	3D Porous Hydrogels	57:75	3D Porous Hydrogels	57:75	Spatial-Controlled Coating of Pro-Angiogenic Proteins on 3D Porous Hydrogels Guides Endothelial Cell Behavior.					
36498931	10	8	theme	proteins	1524:1531	arg1	presence					1507:1514	the presence	1503:1514	the presence of both proteins in the microchannel and in the pores	1503:1568	Most importantly, directed cell migration required the presence of both proteins in the microchannel and in the pores, highlighting the need for an adhesive substrate provided by Caf1-YIGSR for Caf1-VEGF to be effective.					
36498931	6	9	theme	DEAE	1072:1075	arg1	-dextran					1077:1084	diethylaminoethyl (DEAE)-dextran	1053:1084	diethylaminoethyl (DEAE)-dextran	1053:1084	Hydrogel functionalization was achieved by electrostatic interactions via incorporation of diethylaminoethyl (DEAE)-dextran.					
36498931	6	10	theme	-dextran	1077:1084	arg1	incorporation					1036:1048	incorporation	1036:1048	incorporation of diethylaminoethyl (DEAE)-dextran	1036:1084	Hydrogel functionalization was achieved by electrostatic interactions via incorporation of diethylaminoethyl (DEAE)-dextran.					
36498931	6	11	theme	Hydrogel	962:969	arg1	functionalization					971:987	Hydrogel functionalization	962:987	Hydrogel functionalization	962:987	Hydrogel functionalization was achieved by electrostatic interactions via incorporation of diethylaminoethyl (DEAE)-dextran.					
36498931	11	12	theme	pro-angiogenic	1757:1770	arg1	cues					1772:1775	pro-angiogenic cues	1757:1775	pro-angiogenic cues for the study of pro-angiogenic signals	1757:1815	This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials.					
36498931	1	13	theme	biochemical	270:280	arg1	features					282:289	the physical and biochemical features	253:289	the physical and biochemical features of a scaffold	253:303	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	3	14	theme	simplified	572:581	arg1	systems					583:589	simplified systems	572:589	simplified systems	572:589	Numerous strategies in tissue engineering have attempted to control the spatial cues mimicking in vivo angiogenesis by using simplified systems.					
36498931	1	15	dep	composition	138:148	arg1	the					134:136	the	134:136	the	134:136	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	11	16	dep	ability	1701:1707	arg1	guide					1712:1716	guide	1712:1716	to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D	1709:1821	This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials.					
36498931	11	16	dep	ability	1701:1707	arg1	develop					1830:1836	develop	1830:1836	to develop pro-angiogenic implantable materials	1827:1873	This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials.					
36498931	4	17	theme	study	608:612	arg1	aim					596:598	The aim	592:598	The aim of this study	592:612	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36498931	5	18	theme	novel	896:900	arg1	polymers					925:932	novel pro-angiogenic protein polymers	896:932	novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF)	896:959	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	5	18	theme	novel	896:900	arg1	Caf1-VEGF					950:958	Caf1-VEGF	950:958	Caf1-VEGF	950:958	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	5	18	theme	novel	896:900	arg1	Caf1-YIGSR					935:944	Caf1-YIGSR	935:944	Caf1-YIGSR	935:944	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	1	19	dep	cell	334:337	arg1	behavior					339:346	behavior	339:346	behavior	339:346	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	4	20	theme	crosslinked	639:649	arg1	hydrogels					651:659	3D porous crosslinked hydrogels	629:659	3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules	629:723	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36498931	4	21	theme	pro-angiogenic	700:713	arg1	molecules					715:723	pro-angiogenic molecules	700:723	pro-angiogenic molecules	700:723	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36498931	2	22	theme	fundamental	402:412	arg1	role					414:417	a fundamental role	400:417	a fundamental role	400:417	The microenvironment of the ECM plays a fundamental role in regulating angiogenesis.					
36498931	5	23	theme	pharmaceutical-grade	830:849	arg1	pullulan					851:858	pharmaceutical-grade pullulan	830:858	pharmaceutical-grade pullulan	830:858	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	7	24	theme	Caf1	1208:1211	arg1	molecules					1213:1221	Caf1 molecules	1208:1221	Caf1 molecules	1208:1221	Spatial-controlled coating of hydrogels was realized through a combination of freeze-drying and physical absorption with Caf1 molecules.					
36498931	10	25	from	presence	1507:1514	arg1	pores					1564:1568	the pores	1560:1568	the pores	1560:1568	Most importantly, directed cell migration required the presence of both proteins in the microchannel and in the pores, highlighting the need for an adhesive substrate provided by Caf1-YIGSR for Caf1-VEGF to be effective.					
36498931	10	25	from	presence	1507:1514	arg1	microchannel					1540:1551	the microchannel	1536:1551	the microchannel	1536:1551	Most importantly, directed cell migration required the presence of both proteins in the microchannel and in the pores, highlighting the need for an adhesive substrate provided by Caf1-YIGSR for Caf1-VEGF to be effective.					
36498931	1	26	theme	molecular	184:192	arg1	components					194:203	molecular components	184:203	molecular components within the extracellular matrix (ECM)	184:241	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	7	27	theme	physical	1183:1190	arg1	absorption					1192:1201	freeze-drying and physical absorption	1165:1201	freeze-drying and physical absorption	1165:1201	Spatial-controlled coating of hydrogels was realized through a combination of freeze-drying and physical absorption with Caf1 molecules.					
36498931	11	28	theme	pro-angiogenic	1794:1807	arg1	signals					1809:1815	pro-angiogenic signals	1794:1815	pro-angiogenic signals	1794:1815	This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials.					
36498931	1	29	theme	components	194:203	arg1	composition					138:148	composition	138:148	composition	138:148	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	1	29	theme	components	194:203	arg1	arrangement					169:179	the structural arrangement	154:179	the structural arrangement	154:179	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	0	30	theme	Spatial-Controlled	0:17	arg1	Coating					19:25	Spatial-Controlled Coating	0:25	Spatial-Controlled Coating of Pro-Angiogenic Proteins on 3D Porous Hydrogels	0:75	Spatial-Controlled Coating of Pro-Angiogenic Proteins on 3D Porous Hydrogels Guides Endothelial Cell Behavior.					
36498931	10	31	attach	presence	1507:1514	arg1	pores					1564:1568	the pores	1560:1568	the pores	1560:1568	Most importantly, directed cell migration required the presence of both proteins in the microchannel and in the pores, highlighting the need for an adhesive substrate provided by Caf1-YIGSR for Caf1-VEGF to be effective.					
36498931	10	31	attach	presence	1507:1514	arg1	microchannel					1540:1551	the microchannel	1536:1551	the microchannel	1536:1551	Most importantly, directed cell migration required the presence of both proteins in the microchannel and in the pores, highlighting the need for an adhesive substrate provided by Caf1-YIGSR for Caf1-VEGF to be effective.					
36498931	10	31	attach	presence	1507:1514	arg2	proteins					1524:1531	both proteins	1519:1531	both proteins	1519:1531	Most importantly, directed cell migration required the presence of both proteins in the microchannel and in the pores, highlighting the need for an adhesive substrate provided by Caf1-YIGSR for Caf1-VEGF to be effective.					
36498931	7	32	theme	absorption	1192:1201	arg1	combination					1150:1160	a combination	1148:1160	a combination of freeze-drying and physical absorption with Caf1 molecules	1148:1221	Spatial-controlled coating of hydrogels was realized through a combination of freeze-drying and physical absorption with Caf1 molecules.					
36498931	5	33	theme	protein	917:923	arg1	polymers					925:932	novel pro-angiogenic protein polymers	896:932	novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF)	896:959	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	5	33	theme	protein	917:923	arg1	Caf1-VEGF					950:958	Caf1-VEGF	950:958	Caf1-VEGF	950:958	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	5	33	theme	protein	917:923	arg1	Caf1-YIGSR					935:944	Caf1-YIGSR	935:944	Caf1-YIGSR	935:944	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	2	34	theme	ECM	390:392	arg1	microenvironment					366:381	The microenvironment	362:381	The microenvironment of the ECM	362:392	The microenvironment of the ECM plays a fundamental role in regulating angiogenesis.					
36498931	0	35	theme	Pro-Angiogenic	30:43	arg1	Proteins					45:52	Pro-Angiogenic Proteins	30:52	Pro-Angiogenic Proteins	30:52	Spatial-Controlled Coating of Pro-Angiogenic Proteins on 3D Porous Hydrogels Guides Endothelial Cell Behavior.					
36498931	6	36	theme	diethylaminoethyl	1053:1069	arg1	-dextran					1077:1084	diethylaminoethyl (DEAE)-dextran	1053:1084	diethylaminoethyl (DEAE)-dextran	1053:1084	Hydrogel functionalization was achieved by electrostatic interactions via incorporation of diethylaminoethyl (DEAE)-dextran.					
36498931	7	37	theme	freeze-drying	1165:1177	arg1	absorption					1192:1201	freeze-drying and physical absorption	1165:1201	freeze-drying and physical absorption	1165:1201	Spatial-controlled coating of hydrogels was realized through a combination of freeze-drying and physical absorption with Caf1 molecules.					
36498931	3	38	dep	in	542:543	arg1	vivo					545:548	vivo	545:548	vivo	545:548	Numerous strategies in tissue engineering have attempted to control the spatial cues mimicking in vivo angiogenesis by using simplified systems.					
36498931	5	39	with	Hydrogels	766:774	arg1	pores					781:785	pores	781:785	pores	781:785	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	5	39	with	Hydrogels	766:774	arg1	microchannels					801:813	preformed microchannels	791:813	preformed microchannels	791:813	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	7	40	with	combination	1150:1160	arg1	molecules					1213:1221	Caf1 molecules	1208:1221	Caf1 molecules	1208:1221	Spatial-controlled coating of hydrogels was realized through a combination of freeze-drying and physical absorption with Caf1 molecules.					
36498931	1	41	theme	scaffold	296:303	arg1	features					282:289	the physical and biochemical features	253:289	the physical and biochemical features of a scaffold	253:303	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	1	42	dep	determine	243:251	arg1	modulate					325:332	modulate	325:332	modulate cell behavior and function	325:359	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	1	43	theme	tissue	114:119	arg1	engineering					121:131	tissue engineering	114:131	tissue engineering	114:131	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	1	44	theme	extracellular	216:228	arg1	ECM					238:240	ECM	238:240	ECM	238:240	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	1	44	theme	extracellular	216:228	arg1	matrix					230:235	the extracellular matrix	212:235	the extracellular matrix (ECM)	212:241	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function.					
36498931	4	45	theme	cell	746:749	arg1	behavior					756:763	endothelial cell (EC) behavior	734:763	endothelial cell (EC) behavior	734:763	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36498931	10	46	theme	adhesive	1600:1607	arg1	substrate					1609:1617	an adhesive substrate	1597:1617	an adhesive substrate provided by Caf1-YIGSR	1597:1640	Most importantly, directed cell migration required the presence of both proteins in the microchannel and in the pores, highlighting the need for an adhesive substrate provided by Caf1-YIGSR for Caf1-VEGF to be effective.					
36498931	4	47	theme	different	666:674	arg1	presentation					684:695	different spatial presentation	666:695	different spatial presentation of pro-angiogenic molecules	666:723	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36498931	4	48	dep	different	666:674	arg1	spatial					676:682	spatial	676:682	spatial	676:682	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36498931	3	49	theme	Numerous	447:454	arg1	strategies					456:465	Numerous strategies	447:465	Numerous strategies in tissue engineering	447:487	Numerous strategies in tissue engineering have attempted to control the spatial cues mimicking in vivo angiogenesis by using simplified systems.					
36498931	3	50	theme	tissue	470:475	arg1	engineering					477:487	tissue engineering	470:487	tissue engineering	470:487	Numerous strategies in tissue engineering have attempted to control the spatial cues mimicking in vivo angiogenesis by using simplified systems.					
36498931	0	51	theme	Proteins	45:52	arg1	Coating					19:25	Spatial-Controlled Coating	0:25	Spatial-Controlled Coating of Pro-Angiogenic Proteins on 3D Porous Hydrogels	0:75	Spatial-Controlled Coating of Pro-Angiogenic Proteins on 3D Porous Hydrogels Guides Endothelial Cell Behavior.					
36498931	8	52	from	Cells	1224:1228	arg1	scaffolds					1248:1256	functionalized scaffolds	1233:1256	functionalized scaffolds	1233:1256	Cells in functionalized scaffolds survived, adhered, and proliferated over seven days.					
36498931	11	53	theme	signals	1809:1815	arg1	study					1785:1789	the study	1781:1789	the study of pro-angiogenic signals	1781:1815	This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials.					
36498931	10	54	theme	directed	1470:1477	arg1	migration					1484:1492	directed cell migration	1470:1492	directed cell migration	1470:1492	Most importantly, directed cell migration required the presence of both proteins in the microchannel and in the pores, highlighting the need for an adhesive substrate provided by Caf1-YIGSR for Caf1-VEGF to be effective.					
36498931	5	55	theme	pro-angiogenic	902:915	arg1	polymers					925:932	novel pro-angiogenic protein polymers	896:932	novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF)	896:959	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	5	55	theme	pro-angiogenic	902:915	arg1	Caf1-VEGF					950:958	Caf1-VEGF	950:958	Caf1-VEGF	950:958	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	5	55	theme	pro-angiogenic	902:915	arg1	Caf1-YIGSR					935:944	Caf1-YIGSR	935:944	Caf1-YIGSR	935:944	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	0	56	theme	Porous	60:65	arg1	Hydrogels					67:75	3D Porous Hydrogels	57:75	3D Porous Hydrogels	57:75	Spatial-Controlled Coating of Pro-Angiogenic Proteins on 3D Porous Hydrogels Guides Endothelial Cell Behavior.					
36498931	9	57	theme	cell	1362:1365	arg1	adhesion					1367:1374	cell adhesion	1362:1374	cell adhesion	1362:1374	When incorporated alone, Caf1-YIGSR mainly induced cell adhesion and proliferation, whereas Caf1-VEGF promoted cell migration and sprouting.					
36498931	7	58	theme	hydrogels	1117:1125	arg1	coating					1106:1112	Spatial-controlled coating	1087:1112	Spatial-controlled coating of hydrogels	1087:1125	Spatial-controlled coating of hydrogels was realized through a combination of freeze-drying and physical absorption with Caf1 molecules.					
36498931	0	59	theme	3D	57:58	arg1	Hydrogels					67:75	3D Porous Hydrogels	57:75	3D Porous Hydrogels	57:75	Spatial-Controlled Coating of Pro-Angiogenic Proteins on 3D Porous Hydrogels Guides Endothelial Cell Behavior.					
36498931	6	60	theme	electrostatic	1005:1017	arg1	interactions					1019:1030	electrostatic interactions	1005:1030	electrostatic interactions	1005:1030	Hydrogel functionalization was achieved by electrostatic interactions via incorporation of diethylaminoethyl (DEAE)-dextran.					
36498931	11	61	theme	cues	1772:1775	arg1	control					1746:1752	spatial control	1738:1752	spatial control of pro-angiogenic cues for the study of pro-angiogenic signals	1738:1815	This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials.					
36498931	11	62	theme	pro-angiogenic	1838:1851	arg1	materials					1865:1873	pro-angiogenic implantable materials	1838:1873	pro-angiogenic implantable materials	1838:1873	This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials.					
36498931	5	63	dep	polymers	925:932	arg1	polymers					925:932	novel pro-angiogenic protein polymers	896:932	novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF)	896:959	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	5	63	dep	polymers	925:932	arg1	Caf1-VEGF					950:958	Caf1-VEGF	950:958	Caf1-VEGF	950:958	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	5	63	dep	polymers	925:932	arg1	Caf1-YIGSR					935:944	Caf1-YIGSR	935:944	Caf1-YIGSR	935:944	Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF).					
36498931	7	64	theme	Spatial-controlled	1087:1104	arg1	coating					1106:1112	Spatial-controlled coating	1087:1112	Spatial-controlled coating of hydrogels	1087:1125	Spatial-controlled coating of hydrogels was realized through a combination of freeze-drying and physical absorption with Caf1 molecules.					
36498931	3	65	theme	spatial	519:525	arg1	cues					527:530	the spatial cues	515:530	the spatial cues mimicking in vivo angiogenesis by using simplified systems	515:589	Numerous strategies in tissue engineering have attempted to control the spatial cues mimicking in vivo angiogenesis by using simplified systems.					
36498931	11	66	theme	implantable	1853:1863	arg1	materials					1865:1873	pro-angiogenic implantable materials	1838:1873	pro-angiogenic implantable materials	1838:1873	This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials.					
36498931	9	67	theme	cell	1422:1425	arg1	migration					1427:1435	cell migration	1422:1435	cell migration	1422:1435	When incorporated alone, Caf1-YIGSR mainly induced cell adhesion and proliferation, whereas Caf1-VEGF promoted cell migration and sprouting.					
36498931	4	68	with	hydrogels	651:659	arg1	presentation					684:695	different spatial presentation	666:695	different spatial presentation of pro-angiogenic molecules	666:723	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36498931	11	69	theme	spatial	1738:1744	arg1	control					1746:1752	spatial control	1738:1752	spatial control of pro-angiogenic cues for the study of pro-angiogenic signals	1738:1815	This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials.					
36498931	3	70	theme	in	542:543	arg1	angiogenesis					550:561	in vivo angiogenesis	542:561	in vivo angiogenesis	542:561	Numerous strategies in tissue engineering have attempted to control the spatial cues mimicking in vivo angiogenesis by using simplified systems.					
36498931	4	71	theme	porous	632:637	arg1	hydrogels					651:659	3D porous crosslinked hydrogels	629:659	3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules	629:723	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36498931	8	72	theme	functionalized	1233:1246	arg1	scaffolds					1248:1256	functionalized scaffolds	1233:1256	functionalized scaffolds	1233:1256	Cells in functionalized scaffolds survived, adhered, and proliferated over seven days.					
36498931	11	73	theme	EC	1718:1719	arg1	behavior					1721:1728	EC behavior	1718:1728	EC behavior	1718:1728	This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials.					
36498931	4	74	link	crosslinked	639:649	arg1	hydrogels					651:659	3D porous crosslinked hydrogels	629:659	3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules	629:723	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36498931	3	75	from	strategies	456:465	arg1	engineering					477:487	tissue engineering	470:487	tissue engineering	470:487	Numerous strategies in tissue engineering have attempted to control the spatial cues mimicking in vivo angiogenesis by using simplified systems.					
36498931	4	76	theme	molecules	715:723	arg1	presentation					684:695	different spatial presentation	666:695	different spatial presentation of pro-angiogenic molecules	666:723	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36498931	4	77	theme	3D	629:630	arg1	hydrogels					651:659	3D porous crosslinked hydrogels	629:659	3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules	629:723	The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior.					
36835619	0	0	theme	Compounds	93:101	arg1	Encapsulation					25:37	Enhanced Encapsulation	16:37	Enhanced Encapsulation	16:37	3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing.					
36835619	0	0	theme	Compounds	93:101	arg1	Release					54:60	Controlled Release	43:60	Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing	43:118	3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing.					
36835619	4	1	theme	hydrogels	1036:1044	arg1	properties					1011:1020	the anti-inflammatory properties	989:1020	the anti-inflammatory properties of PFs-loaded hydrogels	989:1044	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	4	2	theme	PFs	893:895	arg1	kinetics					919:926	the PFs incorporation/release kinetics	889:926	the PFs incorporation/release kinetics	889:926	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	10	3	theme	inflammation	2042:2053	arg1	process					2055:2061	the inflammation process	2038:2061	the inflammation process	2038:2061	All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications.					
36835619	1	4	theme	wound	158:162	arg1	dressings					164:172	wound dressings	158:172	wound dressings	158:172	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	0	5	from	Release	54:60	arg1	Healing					112:118	Wound Healing	106:118	Wound Healing	106:118	3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing.					
36835619	1	6	theme	unique	247:252	arg1	hydrophilicity					287:300	hydrophilicity	287:300	hydrophilicity	287:300	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	1	6	theme	unique	247:252	arg1	properties					254:263	their unique properties	241:263	their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics	241:402	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	1	6	theme	unique	247:252	arg1	non-toxicity					273:284	non-toxicity	273:284	non-toxicity	273:284	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	1	6	theme	unique	247:252	arg1	biodegradability					324:339	biodegradability	324:339	biodegradability	324:339	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	1	6	theme	unique	247:252	arg1	biocompatibility					303:318	biocompatibility	303:318	biocompatibility	303:318	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	0	7	theme	Bioactive	83:91	arg1	Compounds					93:101	Anti-Inflammatory Bioactive Compounds	65:101	Anti-Inflammatory Bioactive Compounds	65:101	3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing.					
36835619	6	8	from	hydrogels	1393:1401	arg1	increase					1358:1365	the increase	1354:1365	the increase of the dextran content in hydrogels	1354:1401	In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels.					
36835619	6	9	theme	swelling	1298:1305	arg1	degree					1288:1293	an increased degree	1275:1293	an increased degree of swelling and of the encapsulation capacity of PFs	1275:1346	In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels.					
36835619	8	10	dep	hydrogels	1758:1766	arg1	%					1776:1776	over 80%	1769:1776	over 80% viability	1769:1786	Furthermore, CD hydrogels have been shown to promote cell proliferation without cytotoxicity, by successfully culturing fibroblasts and endothelial cells on CD hydrogels (over 80% viability).					
36835619	1	11	theme	dressings	164:172	arg1	development					143:153	the development	139:153	the development of wound dressings	139:172	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	9	12	theme	PFs-loaded	1919:1928	arg1	hydrogels					1930:1938	the PFs-loaded hydrogels	1915:1938	the PFs-loaded hydrogels	1915:1938	The anti-inflammatory tests performed in the presence of lipopolysaccharides demonstrate the anti-inflammatory properties of the PFs-loaded hydrogels.					
36835619	5	13	from	time	1183:1186	arg1	size					1166:1169	the pore size	1157:1169	the pore size at the same time	1157:1186	The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores.					
36835619	0	14	theme	Wound	106:110	arg1	Healing					112:118	Wound Healing	106:118	Wound Healing	106:118	3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing.					
36835619	1	15	theme	advantageous	363:374	arg1	characteristics					388:402	advantageous therapeutic characteristics	363:402	advantageous therapeutic characteristics	363:402	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	9	16	theme	hydrogels	1930:1938	arg1	properties					1901:1910	the anti-inflammatory properties	1879:1910	the anti-inflammatory properties of the PFs-loaded hydrogels	1879:1938	The anti-inflammatory tests performed in the presence of lipopolysaccharides demonstrate the anti-inflammatory properties of the PFs-loaded hydrogels.					
36835619	4	17	theme	swelling	859:866	arg1	degree					868:873	the swelling degree	855:873	the swelling degree of hydrogels	855:886	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	4	18	dep	Fourier	747:753	arg1	spectroscopy					787:798	transformed infrared (ATR-FTIR) spectroscopy	755:798	attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy	719:798	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	4	19	theme	PFs-loaded	1025:1034	arg1	hydrogels					1036:1044	PFs-loaded hydrogels	1025:1044	PFs-loaded hydrogels	1025:1044	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	2	20	theme	anti-inflammatory	521:537	arg1	performance					539:549	their anti-inflammatory performance	515:549	their anti-inflammatory performance	515:549	In this regard, the present study aims to develop hydrogels based on cellulose and dextran (CD) and to reveal their anti-inflammatory performance.					
36835619	1	21	theme	therapeutic	376:386	arg1	characteristics					388:402	advantageous therapeutic characteristics	363:402	advantageous therapeutic characteristics	363:402	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	4	22	theme	incorporation/release	897:917	arg1	kinetics					919:926	the PFs incorporation/release kinetics	889:926	the PFs incorporation/release kinetics	889:926	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	4	23	theme	reflection	736:745	arg1	Fourier					747:753	attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy	719:798	attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy	719:798	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	5	24	theme	pores	1246:1250	arg1	interconnectivity					1221:1237	interconnectivity	1221:1237	interconnectivity	1221:1237	The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores.					
36835619	5	24	theme	pores	1246:1250	arg1	uniformity					1206:1215	uniformity	1206:1215	uniformity	1206:1215	The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores.					
36835619	4	25	theme	anti-inflammatory	993:1009	arg1	properties					1011:1020	the anti-inflammatory properties	989:1020	the anti-inflammatory properties of PFs-loaded hydrogels	989:1044	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	4	26	theme	transformed	755:765	arg1	spectroscopy					787:798	transformed infrared (ATR-FTIR) spectroscopy	755:798	attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy	719:798	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	6	27	theme	increased	1278:1286	arg1	degree					1288:1293	an increased degree	1275:1293	an increased degree of swelling and of the encapsulation capacity of PFs	1275:1346	In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels.					
36835619	10	28	theme	healing	2133:2139	arg1	applications					2141:2152	wound healing applications	2127:2152	wound healing applications	2127:2152	All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications.					
36835619	4	29	theme	scanning	819:826	arg1	SEM					849:851	SEM	849:851	SEM	849:851	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	4	29	theme	scanning	819:826	arg1	microscopy					837:846	scanning electron microscopy	819:846	scanning electron microscopy (SEM)	819:852	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	6	30	theme	content	1382:1388	arg1	increase					1358:1365	the increase	1354:1365	the increase of the dextran content in hydrogels	1354:1401	In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels.					
36835619	9	31	theme	lipopolysaccharides	1847:1865	arg1	presence					1835:1842	the presence	1831:1842	the presence of lipopolysaccharides	1831:1865	The anti-inflammatory tests performed in the presence of lipopolysaccharides demonstrate the anti-inflammatory properties of the PFs-loaded hydrogels.					
36835619	10	32	theme	conclusive	1967:1976	arg1	evidence					1978:1985	conclusive evidence	1967:1985	conclusive evidence on the acceleration of wound healing	1967:2022	All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications.					
36835619	8	33	theme	endothelial	1734:1744	arg1	cells					1746:1750	endothelial cells	1734:1750	endothelial cells	1734:1750	Furthermore, CD hydrogels have been shown to promote cell proliferation without cytotoxicity, by successfully culturing fibroblasts and endothelial cells on CD hydrogels (over 80% viability).					
36835619	5	34	from	impact	1108:1113	arg1	structure					1133:1141	the hydrogel's structure	1118:1141	the hydrogel's structure	1118:1141	The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores.					
36835619	9	35	theme	anti-inflammatory	1794:1810	arg1	tests					1812:1816	The anti-inflammatory tests	1790:1816	The anti-inflammatory tests performed in the presence of lipopolysaccharides	1790:1865	The anti-inflammatory tests performed in the presence of lipopolysaccharides demonstrate the anti-inflammatory properties of the PFs-loaded hydrogels.					
36835619	0	36	from	Encapsulation	25:37	arg1	Healing					112:118	Wound Healing	106:118	Wound Healing	106:118	3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing.					
36835619	10	37	theme	healing	2016:2022	arg1	acceleration					1994:2005	the acceleration	1990:2005	the acceleration of wound healing	1990:2022	All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications.					
36835619	0	38	theme	3D	0:1	arg1	Matrices					3:10	3D Matrices	0:10	3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing.	0:119	3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing.					
36835619	10	39	theme	wound	2010:2014	arg1	healing					2016:2022	wound healing	2010:2022	wound healing	2010:2022	All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications.					
36835619	4	40	theme	hydrogels	878:886	arg1	degree					868:873	the swelling degree	855:873	the swelling degree of hydrogels	855:886	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	3	41	theme	plant	594:598	arg1	PFs					623:625	PFs	623:625	PFs	623:625	This purpose is achieved by incorporating plant bioactive polyphenols (PFs) in CD hydrogels.					
36835619	3	41	theme	plant	594:598	arg1	polyphenols					610:620	plant bioactive polyphenols	594:620	plant bioactive polyphenols (PFs)	594:626	This purpose is achieved by incorporating plant bioactive polyphenols (PFs) in CD hydrogels.					
36835619	4	42	theme	structural	686:695	arg1	characteristics					697:711	the structural characteristics	682:711	the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy	682:798	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	0	43	theme	Enhanced	16:23	arg1	Encapsulation					25:37	Enhanced Encapsulation	16:37	Enhanced Encapsulation	16:37	3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing.					
36835619	1	44	from	trends	129:134	arg1	development					143:153	the development	139:153	the development of wound dressings	139:172	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	6	45	theme	dextran	1374:1380	arg1	content					1382:1388	the dextran content	1370:1388	the dextran content in hydrogels	1370:1401	In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels.					
36835619	4	46	theme	ATR-FTIR	777:784	arg1	spectroscopy					787:798	transformed infrared (ATR-FTIR) spectroscopy	755:798	attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy	719:798	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	5	47	theme	pore	1161:1164	arg1	size					1166:1169	the pore size	1157:1169	the pore size at the same time	1157:1186	The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores.					
36835619	7	48	theme	PFs	1420:1422	arg1	kinetics					1408:1415	The kinetics	1404:1415	The kinetics of PFs released by hydrogels	1404:1444	The kinetics of PFs released by hydrogels was studied according to the Korsmeyer-Peppas model, and it was observed that the transport mechanisms depend on hydrogels' composition and morphology.					
36835619	7	49	theme	transport	1528:1536	arg1	mechanisms					1538:1547	the transport mechanisms	1524:1547	the transport mechanisms	1524:1547	The kinetics of PFs released by hydrogels was studied according to the Korsmeyer-Peppas model, and it was observed that the transport mechanisms depend on hydrogels' composition and morphology.					
36835619	4	50	theme	total	730:734	arg1	Fourier					747:753	attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy	719:798	attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy	719:798	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	6	51	from	content	1382:1388	arg1	hydrogels					1393:1401	hydrogels	1393:1401	hydrogels	1393:1401	In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels.					
36835619	6	52	theme	PFs	1344:1346	arg1	capacity					1332:1339	the encapsulation capacity	1314:1339	the encapsulation capacity of PFs	1314:1346	In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels.					
36835619	10	53	theme	wound	2127:2131	arg1	healing					2133:2139	wound healing	2127:2139	wound healing applications	2127:2152	All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications.					
36835619	8	54	theme	CD	1755:1756	arg1	hydrogels					1758:1766	CD hydrogels	1755:1766	CD hydrogels (over 80% viability)	1755:1787	Furthermore, CD hydrogels have been shown to promote cell proliferation without cytotoxicity, by successfully culturing fibroblasts and endothelial cells on CD hydrogels (over 80% viability).					
36835619	1	55	theme	biopolymer-based	206:221	arg1	materials					223:231	biopolymer-based materials	206:231	biopolymer-based materials	206:231	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	1	56	theme	materials	223:231	arg1	use					199:201	the use	195:201	the use of biopolymer-based materials	195:231	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	5	57	contain	has	1093:1095	arg1	presence					1073:1080	the presence	1069:1080	the presence of dextran	1069:1091	The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores.					
36835619	5	57	contain	has	1093:1095	arg2	impact					1108:1113	a positive impact	1097:1113	a positive impact on the hydrogel's structure	1097:1141	The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores.					
36835619	5	58	theme	same	1178:1181	arg1	time					1183:1186	the same time	1174:1186	the same time	1174:1186	The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores.					
36835619	6	59	theme	capacity	1332:1339	arg1	degree					1288:1293	an increased degree	1275:1293	an increased degree of swelling and of the encapsulation capacity of PFs	1275:1346	In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels.					
36835619	3	60	theme	bioactive	600:608	arg1	PFs					623:625	PFs	623:625	PFs	623:625	This purpose is achieved by incorporating plant bioactive polyphenols (PFs) in CD hydrogels.					
36835619	3	60	theme	bioactive	600:608	arg1	polyphenols					610:620	plant bioactive polyphenols	594:620	plant bioactive polyphenols (PFs)	594:626	This purpose is achieved by incorporating plant bioactive polyphenols (PFs) in CD hydrogels.					
36835619	1	61	theme	Current	121:127	arg1	trends					129:134	Current trends	121:134	Current trends in the development of wound dressings	121:172	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	0	62	theme	Controlled	43:52	arg1	Release					54:60	Controlled Release	43:60	Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing	43:118	3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing.					
36835619	6	63	theme	encapsulation	1318:1330	arg1	capacity					1332:1339	the encapsulation capacity	1314:1339	the encapsulation capacity of PFs	1314:1346	In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels.					
36835619	5	64	theme	dextran	1085:1091	arg1	presence					1073:1080	the presence	1069:1080	the presence of dextran	1069:1091	The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores.					
36835619	1	65	contain	have	358:361	arg1	properties					342:351	properties	342:351	properties that have advantageous therapeutic characteristics	342:402	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	1	65	contain	have	358:361	arg1	non-toxicity					273:284	non-toxicity	273:284	non-toxicity	273:284	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	1	65	contain	have	358:361	arg2	characteristics					388:402	advantageous therapeutic characteristics	363:402	advantageous therapeutic characteristics	363:402	Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics.					
36835619	8	66	theme	cell	1651:1654	arg1	proliferation					1656:1668	cell proliferation	1651:1668	cell proliferation	1651:1668	Furthermore, CD hydrogels have been shown to promote cell proliferation without cytotoxicity, by successfully culturing fibroblasts and endothelial cells on CD hydrogels (over 80% viability).					
36835619	5	67	theme	positive	1099:1106	arg1	impact					1108:1113	a positive impact	1097:1113	a positive impact on the hydrogel's structure	1097:1141	The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores.					
36835619	3	68	theme	CD	631:632	arg1	hydrogels					634:642	CD hydrogels	631:642	CD hydrogels	631:642	This purpose is achieved by incorporating plant bioactive polyphenols (PFs) in CD hydrogels.					
36835619	7	69	theme	Korsmeyer-Peppas	1475:1490	arg1	model					1492:1496	the Korsmeyer-Peppas model	1471:1496	the Korsmeyer-Peppas model	1471:1496	The kinetics of PFs released by hydrogels was studied according to the Korsmeyer-Peppas model, and it was observed that the transport mechanisms depend on hydrogels' composition and morphology.					
36835619	10	70	from	evidence	1978:1985	arg1	acceleration					1994:2005	the acceleration	1990:2005	the acceleration of wound healing	1990:2022	All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications.					
36835619	10	71	from	PFs	2120:2122	arg1	applications					2141:2152	wound healing applications	2127:2152	wound healing applications	2127:2152	All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications.					
36835619	4	72	theme	electron	828:835	arg1	SEM					849:851	SEM	849:851	SEM	849:851	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	4	72	theme	electron	828:835	arg1	microscopy					837:846	scanning electron microscopy	819:846	scanning electron microscopy (SEM)	819:852	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	6	73	from	increase	1358:1365	arg1	hydrogels					1393:1401	hydrogels	1393:1401	hydrogels	1393:1401	In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels.					
36835619	4	74	theme	infrared	767:774	arg1	spectroscopy					787:798	transformed infrared (ATR-FTIR) spectroscopy	755:798	attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy	719:798	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	9	75	theme	anti-inflammatory	1883:1899	arg1	properties					1901:1910	the anti-inflammatory properties	1879:1910	the anti-inflammatory properties of the PFs-loaded hydrogels	1879:1938	The anti-inflammatory tests performed in the presence of lipopolysaccharides demonstrate the anti-inflammatory properties of the PFs-loaded hydrogels.					
36835619	0	76	theme	Anti-Inflammatory	65:81	arg1	Compounds					93:101	Anti-Inflammatory Bioactive Compounds	65:101	Anti-Inflammatory Bioactive Compounds	65:101	3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing.					
36835619	2	77	theme	present	425:431	arg1	study					433:437	the present study	421:437	the present study	421:437	In this regard, the present study aims to develop hydrogels based on cellulose and dextran (CD) and to reveal their anti-inflammatory performance.					
36835619	4	78	theme	attenuated	719:728	arg1	Fourier					747:753	attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy	719:798	attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy	719:798	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	10	79	theme	hydrogels	2092:2100	arg1	use					2079:2081	the use	2075:2081	the use of these hydrogels encapsulated with PFs in wound healing applications	2075:2152	All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications.					
36835619	4	80	theme	properties	1011:1020	arg1	evaluation					975:984	evaluation	975:984	evaluation of the anti-inflammatory properties of PFs-loaded hydrogels	975:1044	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
36835619	5	81	dep	uniformity	1206:1215	arg1	the					1202:1204	the	1202:1204	the	1202:1204	The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores.					
36835619	8	82	theme	CD	1611:1612	arg1	hydrogels					1614:1622	CD hydrogels	1611:1622	CD hydrogels	1611:1622	Furthermore, CD hydrogels have been shown to promote cell proliferation without cytotoxicity, by successfully culturing fibroblasts and endothelial cells on CD hydrogels (over 80% viability).					
36835619	8	83	dep	%	1776:1776	arg1	viability					1778:1786	viability	1778:1786	over 80% viability	1769:1786	Furthermore, CD hydrogels have been shown to promote cell proliferation without cytotoxicity, by successfully culturing fibroblasts and endothelial cells on CD hydrogels (over 80% viability).					
36835619	4	84	dep	together	961:968	arg1	with					970:973	with	970:973	with	970:973	The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels.					
37059955	7	0	theme	adsorption	1077:1086	arg1	results					1099:1105	The adsorption experiment results	1073:1105	The adsorption experiment results	1073:1105	The adsorption experiment results show that the phosphorus removal rate of CS-La-BA can reach 95.6%.					
37059955	4	1	theme	microporous	570:580	arg1	structure					582:590	abundant microporous structure	561:590	abundant microporous structure	561:590	The surface area of BET is 75.46 m2/g and the pore size is mostly at 1.84 nm, indicating that it is a composite porous material with abundant microporous structure.					
37059955	10	2	theme	adsorption	1560:1569	arg1	process					1571:1577	the adsorption process	1556:1577	the adsorption process	1556:1577	The internal diffusion of the adsorption process is dominant, and the maximum adsorption capacity is 31.73 mg/g (25 ℃).					
37059955	5	3	theme	charge	635:640	arg1	property					642:649	the charge property	631:649	the charge property	631:649	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	1	4	theme	lanthanum-modified	115:132	arg1	CS-La-BA					156:163	CS-La-BA	156:163	CS-La-BA	156:163	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA) was prepared with lanthanum chloride, biomass ash, and chitosan to remove phosphorus from water.					
37059955	1	4	theme	lanthanum-modified	115:132	arg1	ball					150:153	The immobilized lanthanum-modified biomass ash gel ball	99:153	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA)	99:164	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA) was prepared with lanthanum chloride, biomass ash, and chitosan to remove phosphorus from water.					
37059955	5	5	theme	La	609:610	arg1	presence					597:604	The presence	593:604	The presence of La on biomass ash and the charge property of CS-La-BA	593:661	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	9	6	theme	adsorption	1410:1419	arg1	process					1421:1427	The phosphorus adsorption process	1395:1427	The phosphorus adsorption process of CS-La-BA	1395:1439	The phosphorus adsorption process of CS-La-BA conforms to the Freundlich isotherm adsorption equation and general-order kinetic model.					
37059955	5	7	theme	biomass	615:621	arg1	ash					623:625	biomass ash	615:625	biomass ash	615:625	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	2	8	theme	analytical	301:310	arg1	techniques					312:321	several analytical techniques	293:321	several analytical techniques	293:321	CS-La-BA was characterized by several analytical techniques.					
37059955	1	9	theme	lanthanum	184:192	arg1	chloride					194:201	lanthanum chloride	184:201	lanthanum chloride	184:201	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA) was prepared with lanthanum chloride, biomass ash, and chitosan to remove phosphorus from water.					
37059955	1	10	theme	biomass	134:140	arg1	CS-La-BA					156:163	CS-La-BA	156:163	CS-La-BA	156:163	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA) was prepared with lanthanum chloride, biomass ash, and chitosan to remove phosphorus from water.					
37059955	1	10	theme	biomass	134:140	arg1	ball					150:153	The immobilized lanthanum-modified biomass ash gel ball	99:153	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA)	99:164	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA) was prepared with lanthanum chloride, biomass ash, and chitosan to remove phosphorus from water.					
37059955	4	11	theme	BET	448:450	arg1	area					440:443	The surface area	428:443	The surface area of BET	428:450	The surface area of BET is 75.46 m2/g and the pore size is mostly at 1.84 nm, indicating that it is a composite porous material with abundant microporous structure.					
37059955	4	11	theme	BET	448:450	arg1	m2/g					461:464	75.46 m2/g	455:464	75.46 m2/g	455:464	The surface area of BET is 75.46 m2/g and the pore size is mostly at 1.84 nm, indicating that it is a composite porous material with abundant microporous structure.					
37059955	10	12	theme	adsorption	1608:1617	arg1	31.73 mg/g					1631:1640	31.73 mg/g	1631:1640	31.73 mg/g (25 ℃)	1631:1647	The internal diffusion of the adsorption process is dominant, and the maximum adsorption capacity is 31.73 mg/g (25 ℃).					
37059955	10	12	theme	adsorption	1608:1617	arg1	capacity					1619:1626	the maximum adsorption capacity	1596:1626	the maximum adsorption capacity	1596:1626	The internal diffusion of the adsorption process is dominant, and the maximum adsorption capacity is 31.73 mg/g (25 ℃).					
37059955	8	13	theme	removal	1245:1251	arg1	rate					1253:1256	the phosphorus removal rate	1230:1256	the phosphorus removal rate	1230:1256	Even after six desorption and regeneration experiments, the phosphorus removal rate still reaches 68.13%, which indicates that CS-La-BA has good phosphorus adsorption performance and desorption and regeneration capacity.					
37059955	5	14	from	mechanism	725:733	arg1	phosphate					750:758	phosphate	750:758	phosphate	750:758	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	9	15	theme	isotherm	1468:1475	arg1	equation					1488:1495	the Freundlich isotherm adsorption equation	1453:1495	the Freundlich isotherm adsorption equation	1453:1495	The phosphorus adsorption process of CS-La-BA conforms to the Freundlich isotherm adsorption equation and general-order kinetic model.					
37059955	1	16	theme	gel	146:148	arg1	CS-La-BA					156:163	CS-La-BA	156:163	CS-La-BA	156:163	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA) was prepared with lanthanum chloride, biomass ash, and chitosan to remove phosphorus from water.					
37059955	1	16	theme	gel	146:148	arg1	ball					150:153	The immobilized lanthanum-modified biomass ash gel ball	99:153	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA)	99:164	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA) was prepared with lanthanum chloride, biomass ash, and chitosan to remove phosphorus from water.					
37059955	1	17	theme	ash	142:144	arg1	CS-La-BA					156:163	CS-La-BA	156:163	CS-La-BA	156:163	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA) was prepared with lanthanum chloride, biomass ash, and chitosan to remove phosphorus from water.					
37059955	1	17	theme	ash	142:144	arg1	ball					150:153	The immobilized lanthanum-modified biomass ash gel ball	99:153	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA)	99:164	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA) was prepared with lanthanum chloride, biomass ash, and chitosan to remove phosphorus from water.					
37059955	5	18	theme	adsorption	714:723	arg1	mechanism					725:733	the adsorption mechanism	710:733	the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism,	710:855	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	5	19	from	phosphate	750:758	arg1	mechanism					725:733	the adsorption mechanism	710:733	the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism,	710:855	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	5	20	theme	electrostatic	786:798	arg1	adsorption					800:809	electrostatic adsorption	786:809	electrostatic adsorption	786:809	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	5	21	from	presence	597:604	arg1	property					642:649	the charge property	631:649	the charge property	631:649	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	5	21	from	presence	597:604	arg1	ash					623:625	biomass ash	615:625	biomass ash	615:625	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	6	22	theme	concentration	956:968	arg1	effects					891:897	The effects	887:897	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA	887:1055	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	8	23	contain	has	1310:1312	arg2	performance					1341:1351	good phosphorus adsorption performance and desorption and regeneration capacity	1314:1392	performance	1341:1351	Even after six desorption and regeneration experiments, the phosphorus removal rate still reaches 68.13%, which indicates that CS-La-BA has good phosphorus adsorption performance and desorption and regeneration capacity.					
37059955	8	23	contain	has	1310:1312	arg2	capacity					1385:1392	good phosphorus adsorption performance and desorption and regeneration capacity	1314:1392	capacity	1385:1392	Even after six desorption and regeneration experiments, the phosphorus removal rate still reaches 68.13%, which indicates that CS-La-BA has good phosphorus adsorption performance and desorption and regeneration capacity.					
37059955	8	23	contain	has	1310:1312	arg1	CS-La-BA					1301:1308	CS-La-BA	1301:1308	CS-La-BA	1301:1308	Even after six desorption and regeneration experiments, the phosphorus removal rate still reaches 68.13%, which indicates that CS-La-BA has good phosphorus adsorption performance and desorption and regeneration capacity.					
37059955	8	23	contain	has	1310:1312	arg2	desorption					1357:1366	good phosphorus adsorption performance and desorption and regeneration capacity	1314:1392	desorption	1357:1366	Even after six desorption and regeneration experiments, the phosphorus removal rate still reaches 68.13%, which indicates that CS-La-BA has good phosphorus adsorption performance and desorption and regeneration capacity.					
37059955	11	24	theme	increase	1760:1767	arg1	process					1701:1707	the adsorption process	1686:1707	the adsorption process of phosphorus by CS-La-BA	1686:1733	Thermodynamic experiments show that the adsorption process of phosphorus by CS-La-BA is a spontaneous entropy increase process.					
37059955	11	24	theme	increase	1760:1767	arg1	process					1769:1775	a spontaneous entropy increase process	1738:1775	a spontaneous entropy increase process	1738:1775	Thermodynamic experiments show that the adsorption process of phosphorus by CS-La-BA is a spontaneous entropy increase process.					
37059955	5	25	from	CS-La-BA	738:745	arg1	phosphate					750:758	phosphate	750:758	phosphate	750:758	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	6	26	theme	initial	937:943	arg1	concentration					956:968	initial phosphorus concentration	937:968	initial phosphorus concentration	937:968	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	11	27	theme	spontaneous	1740:1750	arg1	increase					1760:1767	a spontaneous entropy increase	1738:1767	a spontaneous entropy increase process	1738:1775	Thermodynamic experiments show that the adsorption process of phosphorus by CS-La-BA is a spontaneous entropy increase process.					
37059955	6	28	theme	dosage	929:934	arg1	effects					891:897	The effects	887:897	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA	887:1055	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	7	29	theme	CS-La-BA	1148:1155	arg1	rate					1140:1143	the phosphorus removal rate	1117:1143	the phosphorus removal rate of CS-La-BA	1117:1155	The adsorption experiment results show that the phosphorus removal rate of CS-La-BA can reach 95.6%.					
37059955	6	30	theme	adsorption	971:980	arg1	time					982:985	adsorption time	971:985	adsorption time	971:985	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	6	31	theme	phosphorus	1015:1024	arg1	performance					1033:1043	the phosphorus uptake performance	1011:1043	the phosphorus uptake performance of CS-La-BA	1011:1055	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	4	32	theme	pore	474:477	arg1	size					479:482	the pore size	470:482	the pore size	470:482	The surface area of BET is 75.46 m2/g and the pore size is mostly at 1.84 nm, indicating that it is a composite porous material with abundant microporous structure.					
37059955	8	33	theme	good	1314:1317	arg1	performance					1341:1351	good phosphorus adsorption performance and desorption and regeneration capacity	1314:1392	performance	1341:1351	Even after six desorption and regeneration experiments, the phosphorus removal rate still reaches 68.13%, which indicates that CS-La-BA has good phosphorus adsorption performance and desorption and regeneration capacity.					
37059955	0	34	theme	Adsorption	0:9	arg1	mechanism					11:19	Adsorption mechanism	0:19	Adsorption mechanism of phosphorus on biomass	0:44	Adsorption mechanism of phosphorus on biomass ash modified with lanthanum immobilized by chitosan.					
37059955	3	35	theme	well-developed	367:380	arg1	structure					387:395	a well-developed pore structure	365:395	a well-developed pore structure	365:395	SEM-EDS results showed that CS-La-BA has a well-developed pore structure and abundant adsorption sites.					
37059955	8	36	theme	adsorption	1330:1339	arg1	performance					1341:1351	good phosphorus adsorption performance and desorption and regeneration capacity	1314:1392	performance	1341:1351	Even after six desorption and regeneration experiments, the phosphorus removal rate still reaches 68.13%, which indicates that CS-La-BA has good phosphorus adsorption performance and desorption and regeneration capacity.					
37059955	7	37	theme	phosphorus	1121:1130	arg1	rate					1140:1143	the phosphorus removal rate	1117:1143	the phosphorus removal rate of CS-La-BA	1117:1155	The adsorption experiment results show that the phosphorus removal rate of CS-La-BA can reach 95.6%.					
37059955	1	38	theme	immobilized	103:113	arg1	CS-La-BA					156:163	CS-La-BA	156:163	CS-La-BA	156:163	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA) was prepared with lanthanum chloride, biomass ash, and chitosan to remove phosphorus from water.					
37059955	1	38	theme	immobilized	103:113	arg1	ball					150:153	The immobilized lanthanum-modified biomass ash gel ball	99:153	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA)	99:164	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA) was prepared with lanthanum chloride, biomass ash, and chitosan to remove phosphorus from water.					
37059955	6	39	theme	coexisting	992:1001	arg1	ions					1003:1006	coexisting ions	992:1006	coexisting ions	992:1006	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	4	40	theme	composite	530:538	arg1	material					547:554	a composite porous material	528:554	a composite porous material with abundant microporous structure	528:590	The surface area of BET is 75.46 m2/g and the pore size is mostly at 1.84 nm, indicating that it is a composite porous material with abundant microporous structure.					
37059955	4	40	theme	composite	530:538	arg1	it					522:523	it	522:523	it	522:523	The surface area of BET is 75.46 m2/g and the pore size is mostly at 1.84 nm, indicating that it is a composite porous material with abundant microporous structure.					
37059955	0	41	dep	ash	46:48	arg1	modified					50:57	modified	50:57	ash modified with lanthanum immobilized by chitosan	46:96	Adsorption mechanism of phosphorus on biomass ash modified with lanthanum immobilized by chitosan.					
37059955	3	42	theme	abundant	401:408	arg1	sites					421:425	abundant adsorption sites	401:425	abundant adsorption sites	401:425	SEM-EDS results showed that CS-La-BA has a well-developed pore structure and abundant adsorption sites.					
37059955	6	43	from	effects	891:897	arg1	performance					1033:1043	the phosphorus uptake performance	1011:1043	the phosphorus uptake performance of CS-La-BA	1011:1055	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	4	44	with	material	547:554	arg1	structure					582:590	abundant microporous structure	561:590	abundant microporous structure	561:590	The surface area of BET is 75.46 m2/g and the pore size is mostly at 1.84 nm, indicating that it is a composite porous material with abundant microporous structure.					
37059955	11	45	theme	phosphorus	1712:1721	arg1	process					1701:1707	the adsorption process	1686:1707	the adsorption process of phosphorus by CS-La-BA	1686:1733	Thermodynamic experiments show that the adsorption process of phosphorus by CS-La-BA is a spontaneous entropy increase process.					
37059955	11	45	theme	phosphorus	1712:1721	arg1	process					1769:1775	a spontaneous entropy increase process	1738:1775	a spontaneous entropy increase process	1738:1775	Thermodynamic experiments show that the adsorption process of phosphorus by CS-La-BA is a spontaneous entropy increase process.					
37059955	9	46	theme	general-order	1501:1513	arg1	model					1523:1527	general-order kinetic model	1501:1527	general-order kinetic model	1501:1527	The phosphorus adsorption process of CS-La-BA conforms to the Freundlich isotherm adsorption equation and general-order kinetic model.					
37059955	6	47	theme	CS-La-BA	1048:1055	arg1	performance					1033:1043	the phosphorus uptake performance	1011:1043	the phosphorus uptake performance of CS-La-BA	1011:1055	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	10	48	theme	process	1571:1577	arg1	diffusion					1543:1551	The internal diffusion	1530:1551	The internal diffusion of the adsorption process	1530:1577	The internal diffusion of the adsorption process is dominant, and the maximum adsorption capacity is 31.73 mg/g (25 ℃).					
37059955	10	48	theme	process	1571:1577	arg1	dominant					1582:1589	dominant	1582:1589	dominant	1582:1589	The internal diffusion of the adsorption process is dominant, and the maximum adsorption capacity is 31.73 mg/g (25 ℃).					
37059955	4	49	theme	abundant	561:568	arg1	structure					582:590	abundant microporous structure	561:590	abundant microporous structure	561:590	The surface area of BET is 75.46 m2/g and the pore size is mostly at 1.84 nm, indicating that it is a composite porous material with abundant microporous structure.					
37059955	11	50	theme	Thermodynamic	1650:1662	arg1	experiments					1664:1674	Thermodynamic experiments	1650:1674	Thermodynamic experiments	1650:1674	Thermodynamic experiments show that the adsorption process of phosphorus by CS-La-BA is a spontaneous entropy increase process.					
37059955	9	51	theme	phosphorus	1399:1408	arg1	process					1421:1427	The phosphorus adsorption process	1395:1427	The phosphorus adsorption process of CS-La-BA	1395:1439	The phosphorus adsorption process of CS-La-BA conforms to the Freundlich isotherm adsorption equation and general-order kinetic model.					
37059955	5	52	theme	complexation	833:844	arg1	mechanism					846:854	complexation mechanism	833:854	complexation mechanism	833:854	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	6	53	theme	adsorbent	919:927	arg1	dosage					929:934	adsorbent dosage	919:934	adsorbent dosage	919:934	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	8	54	theme	desorption	1189:1198	arg1	experiments					1217:1227	six desorption and regeneration experiments	1185:1227	six desorption and regeneration experiments	1185:1227	Even after six desorption and regeneration experiments, the phosphorus removal rate still reaches 68.13%, which indicates that CS-La-BA has good phosphorus adsorption performance and desorption and regeneration capacity.					
37059955	5	55	attach	presence	597:604	arg1	property					642:649	the charge property	631:649	the charge property	631:649	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	5	55	attach	presence	597:604	arg1	ash					623:625	biomass ash	615:625	biomass ash	615:625	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	5	55	attach	presence	597:604	arg2	La					609:610	La	609:610	La	609:610	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	2	56	theme	several	293:299	arg1	techniques					312:321	several analytical techniques	293:321	several analytical techniques	293:321	CS-La-BA was characterized by several analytical techniques.					
37059955	9	57	theme	CS-La-BA	1432:1439	arg1	process					1421:1427	The phosphorus adsorption process	1395:1427	The phosphorus adsorption process of CS-La-BA	1395:1439	The phosphorus adsorption process of CS-La-BA conforms to the Freundlich isotherm adsorption equation and general-order kinetic model.					
37059955	6	58	theme	temperature	906:916	arg1	effects					891:897	The effects	887:897	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA	887:1055	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	8	59	theme	regeneration	1204:1215	arg1	experiments					1217:1227	six desorption and regeneration experiments	1185:1227	six desorption and regeneration experiments	1185:1227	Even after six desorption and regeneration experiments, the phosphorus removal rate still reaches 68.13%, which indicates that CS-La-BA has good phosphorus adsorption performance and desorption and regeneration capacity.					
37059955	5	60	theme	ligand	812:817	arg1	exchange					819:826	ligand exchange	812:826	ligand exchange	812:826	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	6	61	theme	pH	902:903	arg1	effects					891:897	The effects	887:897	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA	887:1055	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	10	62	theme	maximum	1600:1606	arg1	31.73 mg/g					1631:1640	31.73 mg/g	1631:1640	31.73 mg/g (25 ℃)	1631:1647	The internal diffusion of the adsorption process is dominant, and the maximum adsorption capacity is 31.73 mg/g (25 ℃).					
37059955	10	62	theme	maximum	1600:1606	arg1	capacity					1619:1626	the maximum adsorption capacity	1596:1626	the maximum adsorption capacity	1596:1626	The internal diffusion of the adsorption process is dominant, and the maximum adsorption capacity is 31.73 mg/g (25 ℃).					
37059955	9	63	theme	Freundlich	1457:1466	arg1	equation					1488:1495	the Freundlich isotherm adsorption equation	1453:1495	the Freundlich isotherm adsorption equation	1453:1495	The phosphorus adsorption process of CS-La-BA conforms to the Freundlich isotherm adsorption equation and general-order kinetic model.					
37059955	4	64	theme	surface	432:438	arg1	area					440:443	The surface area	428:443	The surface area of BET	428:450	The surface area of BET is 75.46 m2/g and the pore size is mostly at 1.84 nm, indicating that it is a composite porous material with abundant microporous structure.					
37059955	4	64	theme	surface	432:438	arg1	m2/g					461:464	75.46 m2/g	455:464	75.46 m2/g	455:464	The surface area of BET is 75.46 m2/g and the pore size is mostly at 1.84 nm, indicating that it is a composite porous material with abundant microporous structure.					
37059955	8	65	theme	phosphorus	1234:1243	arg1	rate					1253:1256	the phosphorus removal rate	1230:1256	the phosphorus removal rate	1230:1256	Even after six desorption and regeneration experiments, the phosphorus removal rate still reaches 68.13%, which indicates that CS-La-BA has good phosphorus adsorption performance and desorption and regeneration capacity.					
37059955	5	66	theme	CS-La-BA	654:661	arg1	property					642:649	the charge property	631:649	the charge property	631:649	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	5	66	theme	CS-La-BA	654:661	arg1	ash					623:625	biomass ash	615:625	biomass ash	615:625	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	9	67	theme	adsorption	1477:1486	arg1	equation					1488:1495	the Freundlich isotherm adsorption equation	1453:1495	the Freundlich isotherm adsorption equation	1453:1495	The phosphorus adsorption process of CS-La-BA conforms to the Freundlich isotherm adsorption equation and general-order kinetic model.					
37059955	6	68	theme	time	982:985	arg1	effects					891:897	The effects	887:897	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA	887:1055	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	9	69	theme	kinetic	1515:1521	arg1	model					1523:1527	general-order kinetic model	1501:1527	general-order kinetic model	1501:1527	The phosphorus adsorption process of CS-La-BA conforms to the Freundlich isotherm adsorption equation and general-order kinetic model.					
37059955	6	70	theme	phosphorus	945:954	arg1	concentration					956:968	initial phosphorus concentration	937:968	initial phosphorus concentration	937:968	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	3	71	theme	SEM-EDS	324:330	arg1	results					332:338	SEM-EDS results	324:338	SEM-EDS results	324:338	SEM-EDS results showed that CS-La-BA has a well-developed pore structure and abundant adsorption sites.					
37059955	0	72	from	mechanism	11:19	arg1	biomass					38:44	biomass	38:44	biomass	38:44	Adsorption mechanism of phosphorus on biomass ash modified with lanthanum immobilized by chitosan.					
37059955	5	73	theme	zeta	690:693	arg1	potential					695:703	zeta potential	690:703	zeta potential	690:703	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	11	74	theme	entropy	1752:1758	arg1	increase					1760:1767	a spontaneous entropy increase	1738:1767	a spontaneous entropy increase process	1738:1775	Thermodynamic experiments show that the adsorption process of phosphorus by CS-La-BA is a spontaneous entropy increase process.					
37059955	6	75	theme	uptake	1026:1031	arg1	performance					1033:1043	the phosphorus uptake performance	1011:1043	the phosphorus uptake performance of CS-La-BA	1011:1055	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	7	76	theme	removal	1132:1138	arg1	rate					1140:1143	the phosphorus removal rate	1117:1143	the phosphorus removal rate of CS-La-BA	1117:1155	The adsorption experiment results show that the phosphorus removal rate of CS-La-BA can reach 95.6%.					
37059955	0	77	theme	phosphorus	24:33	arg1	mechanism					11:19	Adsorption mechanism	0:19	Adsorption mechanism of phosphorus on biomass	0:44	Adsorption mechanism of phosphorus on biomass ash modified with lanthanum immobilized by chitosan.					
37059955	6	78	theme	ions	1003:1006	arg1	effects					891:897	The effects	887:897	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA	887:1055	The effects of pH, temperature, adsorbent dosage, initial phosphorus concentration, adsorption time, and coexisting ions on the phosphorus uptake performance of CS-La-BA were discussed.					
37059955	3	79	theme	pore	382:385	arg1	structure					387:395	a well-developed pore structure	365:395	a well-developed pore structure	365:395	SEM-EDS results showed that CS-La-BA has a well-developed pore structure and abundant adsorption sites.					
37059955	4	80	theme	porous	540:545	arg1	material					547:554	a composite porous material	528:554	a composite porous material with abundant microporous structure	528:590	The surface area of BET is 75.46 m2/g and the pore size is mostly at 1.84 nm, indicating that it is a composite porous material with abundant microporous structure.					
37059955	4	80	theme	porous	540:545	arg1	it					522:523	it	522:523	it	522:523	The surface area of BET is 75.46 m2/g and the pore size is mostly at 1.84 nm, indicating that it is a composite porous material with abundant microporous structure.					
37059955	8	81	theme	phosphorus	1319:1328	arg1	performance					1341:1351	good phosphorus adsorption performance and desorption and regeneration capacity	1314:1392	performance	1341:1351	Even after six desorption and regeneration experiments, the phosphorus removal rate still reaches 68.13%, which indicates that CS-La-BA has good phosphorus adsorption performance and desorption and regeneration capacity.					
37059955	5	82	theme	CS-La-BA	738:745	arg1	mechanism					725:733	the adsorption mechanism	710:733	the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism,	710:855	The presence of La on biomass ash and the charge property of CS-La-BA were determined by XRD and zeta potential, and the adsorption mechanism of CS-La-BA on phosphate, including precipitation, electrostatic adsorption, ligand exchange, and complexation mechanism, was revealed by FTIR and XPS.					
37059955	1	83	theme	biomass	204:210	arg1	ash					212:214	biomass ash	204:214	biomass ash	204:214	The immobilized lanthanum-modified biomass ash gel ball (CS-La-BA) was prepared with lanthanum chloride, biomass ash, and chitosan to remove phosphorus from water.					
37059955	7	84	theme	experiment	1088:1097	arg1	results					1099:1105	The adsorption experiment results	1073:1105	The adsorption experiment results	1073:1105	The adsorption experiment results show that the phosphorus removal rate of CS-La-BA can reach 95.6%.					
37059955	3	85	theme	adsorption	410:419	arg1	sites					421:425	abundant adsorption sites	401:425	abundant adsorption sites	401:425	SEM-EDS results showed that CS-La-BA has a well-developed pore structure and abundant adsorption sites.					
37059955	3	86	contain	has	361:363	arg1	CS-La-BA					352:359	CS-La-BA	352:359	CS-La-BA	352:359	SEM-EDS results showed that CS-La-BA has a well-developed pore structure and abundant adsorption sites.					
37059955	3	86	contain	has	361:363	arg2	sites					421:425	abundant adsorption sites	401:425	abundant adsorption sites	401:425	SEM-EDS results showed that CS-La-BA has a well-developed pore structure and abundant adsorption sites.					
37059955	3	86	contain	has	361:363	arg2	structure					387:395	a well-developed pore structure	365:395	a well-developed pore structure	365:395	SEM-EDS results showed that CS-La-BA has a well-developed pore structure and abundant adsorption sites.					
37059955	11	87	theme	adsorption	1690:1699	arg1	process					1701:1707	the adsorption process	1686:1707	the adsorption process of phosphorus by CS-La-BA	1686:1733	Thermodynamic experiments show that the adsorption process of phosphorus by CS-La-BA is a spontaneous entropy increase process.					
37059955	11	87	theme	adsorption	1690:1699	arg1	process					1769:1775	a spontaneous entropy increase process	1738:1775	a spontaneous entropy increase process	1738:1775	Thermodynamic experiments show that the adsorption process of phosphorus by CS-La-BA is a spontaneous entropy increase process.					
37059955	8	88	theme	regeneration	1372:1383	arg1	capacity					1385:1392	good phosphorus adsorption performance and desorption and regeneration capacity	1314:1392	capacity	1385:1392	Even after six desorption and regeneration experiments, the phosphorus removal rate still reaches 68.13%, which indicates that CS-La-BA has good phosphorus adsorption performance and desorption and regeneration capacity.					
37059955	10	89	theme	internal	1534:1541	arg1	diffusion					1543:1551	The internal diffusion	1530:1551	The internal diffusion of the adsorption process	1530:1577	The internal diffusion of the adsorption process is dominant, and the maximum adsorption capacity is 31.73 mg/g (25 ℃).					
37059955	10	89	theme	internal	1534:1541	arg1	dominant					1582:1589	dominant	1582:1589	dominant	1582:1589	The internal diffusion of the adsorption process is dominant, and the maximum adsorption capacity is 31.73 mg/g (25 ℃).					
35219708	3	0	theme	time	609:612	arg1	effects					554:560	The effects	550:560	The effects of initial metal ion concentration and contact time on the removal of V (V) and Pb (II) by CS-GO and CS-TiO2	550:669	The effects of initial metal ion concentration and contact time on the removal of V (V) and Pb (II) by CS-GO and CS-TiO2 were investigated.					
35219708	2	1	theme	X-ray	510:514	arg1	spectroscopy					530:541	X-ray photoelectron spectroscopy	510:541	X-ray photoelectron spectroscopy (XPS)	510:547	The materials were characterized by scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
35219708	2	1	theme	X-ray	510:514	arg1	XPS					544:546	XPS	544:546	XPS	544:546	The materials were characterized by scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
35219708	9	2	theme	important	1320:1328	arg1	role					1330:1333	an important role	1317:1333	an important role	1317:1333	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	7	3	theme	experimental	1090:1101	arg1	data					1103:1106	the experimental data	1086:1106	the experimental data	1086:1106	the Langmuir adsorption model and Freundlich model agreed well with the experimental data.					
35219708	3	4	theme	concentration	583:595	arg1	effects					554:560	The effects	550:560	The effects of initial metal ion concentration and contact time on the removal of V (V) and Pb (II) by CS-GO and CS-TiO2	550:669	The effects of initial metal ion concentration and contact time on the removal of V (V) and Pb (II) by CS-GO and CS-TiO2 were investigated.					
35219708	8	5	theme	removal	1113:1119	arg1	capacity					1121:1128	The removal capacity	1109:1128	The removal capacity of Pb (II) by CS-GO and CS-TiO2	1109:1160	The removal capacity of Pb (II) by CS-GO and CS-TiO2 were lower than those of V (V).					
35219708	8	5	theme	removal	1113:1119	arg1	lower					1167:1171	lower	1167:1171	lower	1167:1171	The removal capacity of Pb (II) by CS-GO and CS-TiO2 were lower than those of V (V).					
35219708	1	6	theme	chitosan-titanium	206:222	arg1	CS-TiO2					233:239	CS-TiO2	233:239	CS-TiO2	233:239	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	1	6	theme	chitosan-titanium	206:222	arg1	dioxide					224:230	chitosan-titanium dioxide	206:230	chitosan-titanium dioxide (CS-TiO2)	206:240	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	10	7	from	insights	1378:1385	arg1	lead					1403:1406	the removing lead and vanadium pollution	1390:1429	lead	1403:1406	These findings provided insights on the removing lead and vanadium pollution.					
35219708	10	7	from	insights	1378:1385	arg1	pollution					1421:1429	the removing lead and vanadium pollution	1390:1429	pollution	1421:1429	These findings provided insights on the removing lead and vanadium pollution.					
35219708	9	8	theme	adsorptive	1238:1247	arg1	CO					1221:1222	CO	1221:1222	CO	1221:1222	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	9	8	theme	adsorptive	1238:1247	arg1	-OH					1213:1215	The uncross-linked -OH	1194:1215	The uncross-linked -OH	1194:1215	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	9	8	theme	adsorptive	1238:1247	arg1	sites					1249:1253	the main adsorptive sites	1229:1253	the main adsorptive sites for Pb (II) removal	1229:1273	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	0	9	theme	aqueous	34:40	arg1	solution					42:49	aqueous solution	34:49	aqueous solution	34:49	Removal of Pb (II) and V (V) from aqueous solution by glutaraldehyde crosslinked chitosan and nanocomposites.					
35219708	7	10	dep	adsorption	1031:1040	arg1	model					1042:1046	model	1042:1046	model	1042:1046	the Langmuir adsorption model and Freundlich model agreed well with the experimental data.					
35219708	7	10	dep	adsorption	1031:1040	arg1	model					1063:1067	Freundlich model	1052:1067	Freundlich model	1052:1067	the Langmuir adsorption model and Freundlich model agreed well with the experimental data.					
35219708	10	11	theme	removing	1394:1401	arg1	lead					1403:1406	the removing lead and vanadium pollution	1390:1429	lead	1403:1406	These findings provided insights on the removing lead and vanadium pollution.					
35219708	5	12	theme	kinetic	819:825	arg1	models					827:832	the kinetic models	815:832	the kinetic models against experimental data	815:858	A comparison of the kinetic models against experimental data showed that the kinetics react system was best described by the pseudo-second-order model.					
35219708	2	13	dep	transform	466:474	arg1	infrared					476:483	infrared	476:483	transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS)	466:547	The materials were characterized by scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
35219708	1	14	theme	adsorption	314:323	arg1	behavior					325:332	the adsorption behavior	310:332	the adsorption behavior of Pb (II) and V (V) on them	310:361	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	8	15	theme	Pb	1133:1134	arg1	capacity					1121:1128	The removal capacity	1109:1128	The removal capacity of Pb (II) by CS-GO and CS-TiO2	1109:1160	The removal capacity of Pb (II) by CS-GO and CS-TiO2 were lower than those of V (V).					
35219708	8	15	theme	Pb	1133:1134	arg1	lower					1167:1171	lower	1167:1171	lower	1167:1171	The removal capacity of Pb (II) by CS-GO and CS-TiO2 were lower than those of V (V).					
35219708	5	16	theme	models	827:832	arg1	comparison					801:810	A comparison	799:810	A comparison of the kinetic models against experimental data	799:858	A comparison of the kinetic models against experimental data showed that the kinetics react system was best described by the pseudo-second-order model.					
35219708	4	17	theme	hydroxyl	731:738	arg1	GO/TiO2					749:755	GO/TiO2	749:755	GO/TiO2	749:755	Characterization results showed that the hydroxyl group of GO/TiO2 reacted with the amino group of chitosan.					
35219708	4	17	theme	hydroxyl	731:738	arg1	group					740:744	the hydroxyl group	727:744	the hydroxyl group of GO/TiO2	727:755	Characterization results showed that the hydroxyl group of GO/TiO2 reacted with the amino group of chitosan.					
35219708	2	18	theme	electron	427:434	arg1	Fourier					458:464	Fourier	458:464	Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS)	458:547	The materials were characterized by scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
35219708	2	18	theme	electron	427:434	arg1	microscopy					436:445	scanning electron microscopy	418:445	scanning electron microscopy (SEM-EDS)	418:455	The materials were characterized by scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
35219708	2	18	theme	electron	427:434	arg1	SEM-EDS					448:454	SEM-EDS	448:454	SEM-EDS	448:454	The materials were characterized by scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
35219708	4	19	theme	Characterization	690:705	arg1	results					707:713	Characterization results	690:713	Characterization results	690:713	Characterization results showed that the hydroxyl group of GO/TiO2 reacted with the amino group of chitosan.					
35219708	7	20	theme	Freundlich	1052:1061	arg1	model					1063:1067	Freundlich model	1052:1067	Freundlich model	1052:1067	the Langmuir adsorption model and Freundlich model agreed well with the experimental data.					
35219708	1	21	theme	new	125:127	arg1	adsorbents					129:138	new adsorbents	125:138	new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2)	125:240	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	1	22	from	behavior	325:332	arg1	them					358:361	them	358:361	them	358:361	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	4	23	theme	GO/TiO2	749:755	arg1	GO/TiO2					749:755	GO/TiO2	749:755	GO/TiO2	749:755	Characterization results showed that the hydroxyl group of GO/TiO2 reacted with the amino group of chitosan.					
35219708	4	23	theme	GO/TiO2	749:755	arg1	group					740:744	the hydroxyl group	727:744	the hydroxyl group of GO/TiO2	727:755	Characterization results showed that the hydroxyl group of GO/TiO2 reacted with the amino group of chitosan.					
35219708	3	24	from	effects	554:560	arg1	removal					621:627	the removal	617:627	the removal of V (V) and Pb (II) by CS-GO and CS-TiO2	617:669	The effects of initial metal ion concentration and contact time on the removal of V (V) and Pb (II) by CS-GO and CS-TiO2 were investigated.					
35219708	2	25	theme	photoelectron	516:528	arg1	spectroscopy					530:541	X-ray photoelectron spectroscopy	510:541	X-ray photoelectron spectroscopy (XPS)	510:547	The materials were characterized by scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
35219708	2	25	theme	photoelectron	516:528	arg1	XPS					544:546	XPS	544:546	XPS	544:546	The materials were characterized by scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
35219708	5	26	dep	kinetics	876:883	arg1	react					885:889	react	885:889	react system	885:896	A comparison of the kinetic models against experimental data showed that the kinetics react system was best described by the pseudo-second-order model.					
35219708	9	27	theme	uncross-linked	1198:1211	arg1	CO					1221:1222	CO	1221:1222	CO	1221:1222	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	9	27	theme	uncross-linked	1198:1211	arg1	-OH					1213:1215	The uncross-linked -OH	1194:1215	The uncross-linked -OH	1194:1215	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	9	27	theme	uncross-linked	1198:1211	arg1	sites					1249:1253	the main adsorptive sites	1229:1253	the main adsorptive sites for Pb (II) removal	1229:1273	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	1	28	theme	high	145:148	arg1	strength					161:168	high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2)	145:240	high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2)	145:240	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	0	29	theme	Pb	11:12	arg1	Removal					0:6	Removal	0:6	Removal of Pb (II) and V (V) from aqueous solution by glutaraldehyde	0:67	Removal of Pb (II) and V (V) from aqueous solution by glutaraldehyde crosslinked chitosan and nanocomposites.					
35219708	3	30	theme	V	632:632	arg1	removal					621:627	the removal	617:627	the removal of V (V) and Pb (II) by CS-GO and CS-TiO2	617:669	The effects of initial metal ion concentration and contact time on the removal of V (V) and Pb (II) by CS-GO and CS-TiO2 were investigated.					
35219708	4	31	theme	amino	774:778	arg1	group					780:784	the amino group	770:784	the amino group of chitosan	770:796	Characterization results showed that the hydroxyl group of GO/TiO2 reacted with the amino group of chitosan.					
35219708	4	31	theme	amino	774:778	arg1	chitosan					789:796	chitosan	789:796	chitosan	789:796	Characterization results showed that the hydroxyl group of GO/TiO2 reacted with the amino group of chitosan.					
35219708	1	32	theme	Pb	337:338	arg1	behavior					325:332	the adsorption behavior	310:332	the adsorption behavior of Pb (II) and V (V) on them	310:361	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	1	33	theme	mechanical	150:159	arg1	strength					161:168	high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2)	145:240	high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2)	145:240	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	3	34	theme	initial	565:571	arg1	concentration					583:595	initial metal ion concentration	565:595	initial metal ion concentration	565:595	The effects of initial metal ion concentration and contact time on the removal of V (V) and Pb (II) by CS-GO and CS-TiO2 were investigated.					
35219708	4	35	theme	chitosan	789:796	arg1	group					780:784	the amino group	770:784	the amino group of chitosan	770:796	Characterization results showed that the hydroxyl group of GO/TiO2 reacted with the amino group of chitosan.					
35219708	4	35	theme	chitosan	789:796	arg1	chitosan					789:796	chitosan	789:796	chitosan	789:796	Characterization results showed that the hydroxyl group of GO/TiO2 reacted with the amino group of chitosan.					
35219708	5	36	theme	pseudo-second-order	924:942	arg1	model					944:948	the pseudo-second-order model	920:948	the pseudo-second-order model	920:948	A comparison of the kinetic models against experimental data showed that the kinetics react system was best described by the pseudo-second-order model.					
35219708	10	37	theme	vanadium	1412:1419	arg1	pollution					1421:1429	the removing lead and vanadium pollution	1390:1429	pollution	1421:1429	These findings provided insights on the removing lead and vanadium pollution.					
35219708	7	38	theme	Langmuir	1022:1029	arg1	adsorption					1031:1040	the Langmuir adsorption	1018:1040	the Langmuir adsorption model and Freundlich model	1018:1067	the Langmuir adsorption model and Freundlich model agreed well with the experimental data.					
35219708	9	39	theme	main	1233:1236	arg1	CO					1221:1222	CO	1221:1222	CO	1221:1222	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	9	39	theme	main	1233:1236	arg1	-OH					1213:1215	The uncross-linked -OH	1194:1215	The uncross-linked -OH	1194:1215	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	9	39	theme	main	1233:1236	arg1	sites					1249:1253	the main adsorptive sites	1229:1253	the main adsorptive sites for Pb (II) removal	1229:1273	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	2	40	dep	Fourier	458:464	arg1	transform					466:474	transform	466:474	transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS)	466:547	The materials were characterized by scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
35219708	0	41	theme	V	23:23	arg1	Removal					0:6	Removal	0:6	Removal of Pb (II) and V (V) from aqueous solution by glutaraldehyde	0:67	Removal of Pb (II) and V (V) from aqueous solution by glutaraldehyde crosslinked chitosan and nanocomposites.					
35219708	1	42	with	adsorbents	129:138	arg1	strength					161:168	high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2)	145:240	high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2)	145:240	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	9	43	link	uncross-linked	1198:1211	arg1	CO					1221:1222	CO	1221:1222	CO	1221:1222	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	9	43	link	uncross-linked	1198:1211	arg1	-OH					1213:1215	The uncross-linked -OH	1194:1215	The uncross-linked -OH	1194:1215	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	9	43	link	uncross-linked	1198:1211	arg1	sites					1249:1253	the main adsorptive sites	1229:1253	the main adsorptive sites for Pb (II) removal	1229:1273	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	6	44	theme	adsorption	1000:1009	arg1	force					1011:1015	the main adsorption force	991:1015	the main adsorption force	991:1015	indicating that chemical adsorption was the main adsorption force.					
35219708	6	44	theme	adsorption	1000:1009	arg1	adsorption					976:985	chemical adsorption	967:985	chemical adsorption	967:985	indicating that chemical adsorption was the main adsorption force.					
35219708	3	45	theme	contact	601:607	arg1	time					609:612	contact time	601:612	contact time	601:612	The effects of initial metal ion concentration and contact time on the removal of V (V) and Pb (II) by CS-GO and CS-TiO2 were investigated.					
35219708	3	46	theme	metal	573:577	arg1	concentration					583:595	initial metal ion concentration	565:595	initial metal ion concentration	565:595	The effects of initial metal ion concentration and contact time on the removal of V (V) and Pb (II) by CS-GO and CS-TiO2 were investigated.					
35219708	3	47	theme	ion	579:581	arg1	concentration					583:595	initial metal ion concentration	565:595	initial metal ion concentration	565:595	The effects of initial metal ion concentration and contact time on the removal of V (V) and Pb (II) by CS-GO and CS-TiO2 were investigated.					
35219708	9	48	theme	uncross-linked	1282:1295	arg1	-OH					1297:1299	uncross-linked -OH	1282:1299	uncross-linked -OH	1282:1299	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	6	49	theme	main	995:998	arg1	force					1011:1015	the main adsorption force	991:1015	the main adsorption force	991:1015	indicating that chemical adsorption was the main adsorption force.					
35219708	6	49	theme	main	995:998	arg1	adsorption					976:985	chemical adsorption	967:985	chemical adsorption	967:985	indicating that chemical adsorption was the main adsorption force.					
35219708	3	50	theme	Pb	642:643	arg1	removal					621:627	the removal	617:627	the removal of V (V) and Pb (II) by CS-GO and CS-TiO2	617:669	The effects of initial metal ion concentration and contact time on the removal of V (V) and Pb (II) by CS-GO and CS-TiO2 were investigated.					
35219708	9	51	link	uncross-linked	1282:1295	arg1	-OH					1297:1299	uncross-linked -OH	1282:1299	uncross-linked -OH	1282:1299	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	1	52	theme	V	349:349	arg1	behavior					325:332	the adsorption behavior	310:332	the adsorption behavior of Pb (II) and V (V) on them	310:361	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	1	53	dep	strength	161:168	arg1	CS-GO					195:199	CS-GO	195:199	CS-GO	195:199	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	1	53	dep	strength	161:168	arg1	oxide					188:192	chitosan-graphene oxide	170:192	chitosan-graphene oxide (CS-GO)	170:200	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	1	53	dep	strength	161:168	arg1	dioxide					224:230	chitosan-titanium dioxide	206:230	chitosan-titanium dioxide (CS-TiO2)	206:240	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	1	53	dep	strength	161:168	arg1	CS-TiO2					233:239	CS-TiO2	233:239	CS-TiO2	233:239	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	0	54	from	solution	42:49	arg1	Removal					0:6	Removal	0:6	Removal of Pb (II) and V (V) from aqueous solution by glutaraldehyde	0:67	Removal of Pb (II) and V (V) from aqueous solution by glutaraldehyde crosslinked chitosan and nanocomposites.					
35219708	2	55	theme	scanning	418:425	arg1	Fourier					458:464	Fourier	458:464	Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS)	458:547	The materials were characterized by scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
35219708	2	55	theme	scanning	418:425	arg1	microscopy					436:445	scanning electron microscopy	418:445	scanning electron microscopy (SEM-EDS)	418:455	The materials were characterized by scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
35219708	2	55	theme	scanning	418:425	arg1	SEM-EDS					448:454	SEM-EDS	448:454	SEM-EDS	448:454	The materials were characterized by scanning electron microscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
35219708	1	56	theme	chitosan-graphene	170:186	arg1	CS-GO					195:199	CS-GO	195:199	CS-GO	195:199	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	1	56	theme	chitosan-graphene	170:186	arg1	oxide					188:192	chitosan-graphene oxide	170:192	chitosan-graphene oxide (CS-GO)	170:200	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	9	57	theme	Pb	1259:1260	arg1	removal					1267:1273	Pb (II) removal	1259:1273	Pb (II) removal	1259:1273	The uncross-linked -OH and CO were the main adsorptive sites for Pb (II) removal, while uncross-linked -OH and -NH2 played an important role in removing V (V).					
35219708	5	58	theme	experimental	842:853	arg1	data					855:858	experimental data	842:858	experimental data	842:858	A comparison of the kinetic models against experimental data showed that the kinetics react system was best described by the pseudo-second-order model.					
35219708	1	59	theme	crosslinking	286:297	arg1	agent					299:303	crosslinking agent	286:303	crosslinking agent	286:303	In this paper, new adsorbents with high mechanical strength chitosan-graphene oxide (CS-GO) and chitosan-titanium dioxide (CS-TiO2) were synthesized by using glutaraldehyde as crosslinking agent, and the adsorption behavior of Pb (II) and V (V) on them were investigated.					
35219708	6	60	theme	chemical	967:974	arg1	force					1011:1015	the main adsorption force	991:1015	the main adsorption force	991:1015	indicating that chemical adsorption was the main adsorption force.					
35219708	6	60	theme	chemical	967:974	arg1	adsorption					976:985	chemical adsorption	967:985	chemical adsorption	967:985	indicating that chemical adsorption was the main adsorption force.					
37120221	2	0	theme	simulated	639:647	arg1	model					667:671	a simulated gastric-intestine model	637:671	a simulated gastric-intestine model	637:671	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	2	1	theme	physicochemical	378:392	arg1	properties					394:403	physicochemical properties	378:403	physicochemical properties	378:403	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	9	2	theme	modified	1786:1793	arg1	starch					1802:1807	modified porous starch	1786:1807	modified porous starch	1786:1807	It provides theoretical significance and data basis for the encapsulation of active substances by modified porous starch.					
37120221	7	3	theme	radical	1483:1489	arg1	scavenging					1491:1500	free radical scavenging	1478:1500	free radical scavenging	1478:1500	To recapitulate, two different modified porous starch microspheres improved the drug loading, slow release and free radical scavenging effects of curcumin.					
37120221	4	4	theme	decomposition	907:919	arg1	temperature					921:931	the initial decomposition temperature	895:931	the initial decomposition temperature	895:931	Microspheres increased the initial decomposition temperature of curcumin, which has a protective effect on curcumin.					
37120221	3	5	theme	hydrogen	765:772	arg1	formation					779:787	the composite and hydrogen bond formation	747:787	formation	779:787	The FT-IR results revealed that curcumin was amorphously encapsulated in the composite and hydrogen bond formation between starch and curcumin was one of the major driving forces for encapsulation.					
37120221	6	6	theme	curcumin	1357:1364	arg1	release					1346:1352	controlled release	1335:1352	controlled release of curcumin	1335:1364	The release mechanism of curcumin from microspheres fits first-order and Higuchi models well in gastric and intestinal models, respectively, indicating that encapsulation of curcumin within different porous starches microspheres enables controlled release of curcumin.					
37120221	6	7	from	microspheres	1137:1148	arg1	mechanism					1110:1118	The release mechanism	1098:1118	The release mechanism of curcumin from microspheres	1098:1148	The release mechanism of curcumin from microspheres fits first-order and Higuchi models well in gastric and intestinal models, respectively, indicating that encapsulation of curcumin within different porous starches microspheres enables controlled release of curcumin.					
37120221	9	8	theme	porous	1795:1800	arg1	starch					1802:1807	modified porous starch	1786:1807	modified porous starch	1786:1807	It provides theoretical significance and data basis for the encapsulation of active substances by modified porous starch.					
37120221	7	9	theme	curcumin	1513:1520	arg1	effects					1502:1508	the drug loading, slow release and free radical scavenging effects	1443:1508	the drug loading, slow release and free radical scavenging effects of curcumin	1443:1520	To recapitulate, two different modified porous starch microspheres improved the drug loading, slow release and free radical scavenging effects of curcumin.					
37120221	9	10	theme	significance	1712:1723	arg1	basis					1734:1738	theoretical significance and data basis	1700:1738	theoretical significance and data basis for the encapsulation of active substances by modified porous starch	1700:1807	It provides theoretical significance and data basis for the encapsulation of active substances by modified porous starch.					
37120221	0	11	theme	curcumin	118:125	arg1	properties					104:113	properties	104:113	properties of curcumin	104:125	Modified porous starches loading curcumin and improving the free radical scavenging ability and release properties of curcumin.					
37120221	1	12	theme	porous	228:233	arg1	starch					235:240	cross-linked porous starch	215:240	cross-linked porous starch	215:240	Maize porous starch-curcumin microspheres were prepared by encapsulating curcumin into cross-linked porous starch and oxidized porous starch to investigate the effect of modified porous starch in embedding and protecting curcumin.					
37120221	8	13	theme	starch	1559:1564	arg1	microspheres					1566:1577	the cross-linked porous starch microspheres	1535:1577	the cross-linked porous starch microspheres	1535:1577	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
37120221	9	14	theme	substances	1772:1781	arg1	encapsulation					1748:1760	the encapsulation	1744:1760	the encapsulation of active substances by modified porous starch	1744:1807	It provides theoretical significance and data basis for the encapsulation of active substances by modified porous starch.					
37120221	4	15	theme	protective	958:967	arg1	effect					969:974	a protective effect	956:974	a protective effect	956:974	Microspheres increased the initial decomposition temperature of curcumin, which has a protective effect on curcumin.					
37120221	6	16	theme	controlled	1335:1344	arg1	release					1346:1352	controlled release	1335:1352	controlled release of curcumin	1335:1364	The release mechanism of curcumin from microspheres fits first-order and Higuchi models well in gastric and intestinal models, respectively, indicating that encapsulation of curcumin within different porous starches microspheres enables controlled release of curcumin.					
37120221	1	17	theme	porous	134:139	arg1	microspheres					157:168	Maize porous starch-curcumin microspheres	128:168	Maize porous starch-curcumin microspheres	128:168	Maize porous starch-curcumin microspheres were prepared by encapsulating curcumin into cross-linked porous starch and oxidized porous starch to investigate the effect of modified porous starch in embedding and protecting curcumin.					
37120221	6	18	theme	release	1102:1108	arg1	mechanism					1110:1118	The release mechanism	1098:1118	The release mechanism of curcumin from microspheres	1098:1148	The release mechanism of curcumin from microspheres fits first-order and Higuchi models well in gastric and intestinal models, respectively, indicating that encapsulation of curcumin within different porous starches microspheres enables controlled release of curcumin.					
37120221	8	19	theme	slow	1608:1611	arg1	ability					1621:1627	slow release ability	1608:1627	slow release ability	1608:1627	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
37120221	7	20	theme	slow	1461:1464	arg1	release					1466:1472	slow release	1461:1472	slow release	1461:1472	To recapitulate, two different modified porous starch microspheres improved the drug loading, slow release and free radical scavenging effects of curcumin.					
37120221	3	21	theme	driving	838:844	arg1	forces					846:851	the major driving forces	828:851	the major driving forces for encapsulation	828:869	The FT-IR results revealed that curcumin was amorphously encapsulated in the composite and hydrogen bond formation between starch and curcumin was one of the major driving forces for encapsulation.					
37120221	0	22	theme	free	60:63	arg1	radical					65:71	the free radical	56:71	the free radical scavenging ability	56:90	Modified porous starches loading curcumin and improving the free radical scavenging ability and release properties of curcumin.					
37120221	0	23	theme	scavenging	73:82	arg1	ability					84:90	the free radical scavenging ability	56:90	the free radical scavenging ability	56:90	Modified porous starches loading curcumin and improving the free radical scavenging ability and release properties of curcumin.					
37120221	8	24	link	cross-linked	1539:1550	arg1	microspheres					1566:1577	the cross-linked porous starch microspheres	1535:1577	the cross-linked porous starch microspheres	1535:1577	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
37120221	6	25	theme	starches	1305:1312	arg1	microspheres					1314:1325	different porous starches microspheres	1288:1325	different porous starches microspheres	1288:1325	The release mechanism of curcumin from microspheres fits first-order and Higuchi models well in gastric and intestinal models, respectively, indicating that encapsulation of curcumin within different porous starches microspheres enables controlled release of curcumin.					
37120221	1	26	link	cross-linked	215:226	arg1	starch					235:240	cross-linked porous starch	215:240	cross-linked porous starch	215:240	Maize porous starch-curcumin microspheres were prepared by encapsulating curcumin into cross-linked porous starch and oxidized porous starch to investigate the effect of modified porous starch in embedding and protecting curcumin.					
37120221	5	27	theme	scavenging	1048:1057	arg1	ability					1072:1078	the scavenging free radical ability	1044:1078	the scavenging free radical ability of porous starch	1044:1095	Modification improved the encapsulation efficiency and the scavenging free radical ability of porous starch.					
37120221	2	28	theme	scanning	441:448	arg1	microscopy					459:468	scanning electron microscopy	441:468	scanning electron microscopy	441:468	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	2	29	dep	transform	479:487	arg1	infrared					489:496	infrared	489:496	transform infrared spectroscopy (FT-IR)	479:517	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	5	30	theme	radical	1064:1070	arg1	ability					1072:1078	the scavenging free radical ability	1044:1078	the scavenging free radical ability of porous starch	1044:1095	Modification improved the encapsulation efficiency and the scavenging free radical ability of porous starch.					
37120221	8	31	theme	porous	1660:1665	arg1	microspheres					1674:1685	the oxidized porous starch microspheres	1647:1685	the oxidized porous starch microspheres	1647:1685	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
37120221	0	32	theme	Modified	0:7	arg1	starches					16:23	Modified porous starches	0:23	Modified porous starches	0:23	Modified porous starches loading curcumin and improving the free radical scavenging ability and release properties of curcumin.					
37120221	1	33	theme	modified	298:305	arg1	starch					314:319	modified porous starch	298:319	modified porous starch	298:319	Maize porous starch-curcumin microspheres were prepared by encapsulating curcumin into cross-linked porous starch and oxidized porous starch to investigate the effect of modified porous starch in embedding and protecting curcumin.					
37120221	5	34	theme	encapsulation	1015:1027	arg1	efficiency					1029:1038	the encapsulation efficiency	1011:1038	the encapsulation efficiency	1011:1038	Modification improved the encapsulation efficiency and the scavenging free radical ability of porous starch.					
37120221	7	35	theme	drug	1447:1450	arg1	loading					1452:1458	drug loading	1447:1458	drug loading	1447:1458	To recapitulate, two different modified porous starch microspheres improved the drug loading, slow release and free radical scavenging effects of curcumin.					
37120221	6	36	theme	porous	1298:1303	arg1	microspheres					1314:1325	different porous starches microspheres	1288:1325	different porous starches microspheres	1288:1325	The release mechanism of curcumin from microspheres fits first-order and Higuchi models well in gastric and intestinal models, respectively, indicating that encapsulation of curcumin within different porous starches microspheres enables controlled release of curcumin.					
37120221	1	37	theme	starch	314:319	arg1	effect					288:293	the effect	284:293	the effect of modified porous starch in embedding	284:332	Maize porous starch-curcumin microspheres were prepared by encapsulating curcumin into cross-linked porous starch and oxidized porous starch to investigate the effect of modified porous starch in embedding and protecting curcumin.					
37120221	9	38	theme	data	1729:1732	arg1	basis					1734:1738	theoretical significance and data basis	1700:1738	theoretical significance and data basis for the encapsulation of active substances by modified porous starch	1700:1807	It provides theoretical significance and data basis for the encapsulation of active substances by modified porous starch.					
37120221	2	39	theme	X-ray	520:524	arg1	diffraction					526:536	X-ray diffraction	520:536	X-ray diffraction	520:536	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	6	40	theme	curcumin	1123:1130	arg1	mechanism					1110:1118	The release mechanism	1098:1118	The release mechanism of curcumin from microspheres	1098:1148	The release mechanism of curcumin from microspheres fits first-order and Higuchi models well in gastric and intestinal models, respectively, indicating that encapsulation of curcumin within different porous starches microspheres enables controlled release of curcumin.					
37120221	7	41	theme	starch	1414:1419	arg1	microspheres					1421:1432	two different modified porous starch microspheres	1384:1432	two different modified porous starch microspheres	1384:1432	To recapitulate, two different modified porous starch microspheres improved the drug loading, slow release and free radical scavenging effects of curcumin.					
37120221	2	42	dep	Fourier	471:477	arg1	transform					479:487	transform	479:487	transform infrared spectroscopy (FT-IR)	479:517	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	7	43	theme	modified	1398:1405	arg1	microspheres					1421:1432	two different modified porous starch microspheres	1384:1432	two different modified porous starch microspheres	1384:1432	To recapitulate, two different modified porous starch microspheres improved the drug loading, slow release and free radical scavenging effects of curcumin.					
37120221	6	44	theme	different	1288:1296	arg1	microspheres					1314:1325	different porous starches microspheres	1288:1325	different porous starches microspheres	1288:1325	The release mechanism of curcumin from microspheres fits first-order and Higuchi models well in gastric and intestinal models, respectively, indicating that encapsulation of curcumin within different porous starches microspheres enables controlled release of curcumin.					
37120221	1	45	theme	oxidized	246:253	arg1	starch					262:267	oxidized porous starch	246:267	oxidized porous starch	246:267	Maize porous starch-curcumin microspheres were prepared by encapsulating curcumin into cross-linked porous starch and oxidized porous starch to investigate the effect of modified porous starch in embedding and protecting curcumin.					
37120221	9	46	theme	active	1765:1770	arg1	substances					1772:1781	active substances	1765:1781	active substances	1765:1781	It provides theoretical significance and data basis for the encapsulation of active substances by modified porous starch.					
37120221	8	47	theme	porous	1552:1557	arg1	microspheres					1566:1577	the cross-linked porous starch microspheres	1535:1577	the cross-linked porous starch microspheres	1535:1577	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
37120221	2	48	theme	microspheres	408:419	arg1	morphology					363:372	morphology	363:372	morphology	363:372	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	2	48	theme	microspheres	408:419	arg1	properties					394:403	physicochemical properties	378:403	physicochemical properties	378:403	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	5	49	theme	starch	1090:1095	arg1	efficiency					1029:1038	the encapsulation efficiency	1011:1038	the encapsulation efficiency	1011:1038	Modification improved the encapsulation efficiency and the scavenging free radical ability of porous starch.					
37120221	5	49	theme	starch	1090:1095	arg1	ability					1072:1078	the scavenging free radical ability	1044:1078	the scavenging free radical ability of porous starch	1044:1095	Modification improved the encapsulation efficiency and the scavenging free radical ability of porous starch.					
37120221	8	50	contain	had	1579:1581	arg2	ability					1621:1627	slow release ability	1608:1627	slow release ability	1608:1627	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
37120221	8	50	contain	had	1579:1581	arg1	microspheres					1566:1577	the cross-linked porous starch microspheres	1535:1577	the cross-linked porous starch microspheres	1535:1577	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
37120221	8	50	contain	had	1579:1581	arg2	encapsulation					1590:1602	higher encapsulation	1583:1602	higher encapsulation	1583:1602	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
37120221	4	51	theme	curcumin	936:943	arg1	temperature					921:931	the initial decomposition temperature	895:931	the initial decomposition temperature	895:931	Microspheres increased the initial decomposition temperature of curcumin, which has a protective effect on curcumin.					
37120221	6	52	theme	curcumin	1272:1279	arg1	encapsulation					1255:1267	encapsulation	1255:1267	encapsulation of curcumin within different porous starches microspheres	1255:1325	The release mechanism of curcumin from microspheres fits first-order and Higuchi models well in gastric and intestinal models, respectively, indicating that encapsulation of curcumin within different porous starches microspheres enables controlled release of curcumin.					
37120221	4	53	theme	initial	899:905	arg1	temperature					921:931	the initial decomposition temperature	895:931	the initial decomposition temperature	895:931	Microspheres increased the initial decomposition temperature of curcumin, which has a protective effect on curcumin.					
37120221	3	54	theme	bond	774:777	arg1	formation					779:787	the composite and hydrogen bond formation	747:787	formation	779:787	The FT-IR results revealed that curcumin was amorphously encapsulated in the composite and hydrogen bond formation between starch and curcumin was one of the major driving forces for encapsulation.					
37120221	2	55	theme	gastric-intestine	649:665	arg1	model					667:671	a simulated gastric-intestine model	637:671	a simulated gastric-intestine model	637:671	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	1	56	from	effect	288:293	arg1	embedding					324:332	embedding	324:332	embedding	324:332	Maize porous starch-curcumin microspheres were prepared by encapsulating curcumin into cross-linked porous starch and oxidized porous starch to investigate the effect of modified porous starch in embedding and protecting curcumin.					
37120221	1	57	theme	cross-linked	215:226	arg1	starch					235:240	cross-linked porous starch	215:240	cross-linked porous starch	215:240	Maize porous starch-curcumin microspheres were prepared by encapsulating curcumin into cross-linked porous starch and oxidized porous starch to investigate the effect of modified porous starch in embedding and protecting curcumin.					
37120221	2	58	theme	antioxidant	572:582	arg1	activity					584:591	antioxidant activity	572:591	antioxidant activity	572:591	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	8	59	theme	higher	1583:1588	arg1	encapsulation					1590:1602	higher encapsulation	1583:1602	higher encapsulation	1583:1602	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
37120221	3	60	theme	major	832:836	arg1	forces					846:851	the major driving forces	828:851	the major driving forces for encapsulation	828:869	The FT-IR results revealed that curcumin was amorphously encapsulated in the composite and hydrogen bond formation between starch and curcumin was one of the major driving forces for encapsulation.					
37120221	2	61	theme	curcumin	609:616	arg1	release					598:604	the release	594:604	the release of curcumin	594:616	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	7	62	theme	scavenging	1491:1500	arg1	effects					1502:1508	the drug loading, slow release and free radical scavenging effects	1443:1508	the drug loading, slow release and free radical scavenging effects of curcumin	1443:1520	To recapitulate, two different modified porous starch microspheres improved the drug loading, slow release and free radical scavenging effects of curcumin.					
37120221	1	63	theme	Maize	128:132	arg1	microspheres					157:168	Maize porous starch-curcumin microspheres	128:168	Maize porous starch-curcumin microspheres	128:168	Maize porous starch-curcumin microspheres were prepared by encapsulating curcumin into cross-linked porous starch and oxidized porous starch to investigate the effect of modified porous starch in embedding and protecting curcumin.					
37120221	8	64	theme	release	1613:1619	arg1	ability					1621:1627	slow release ability	1608:1627	slow release ability	1608:1627	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
37120221	7	65	theme	free	1478:1481	arg1	scavenging					1491:1500	free radical scavenging	1478:1500	free radical scavenging	1478:1500	To recapitulate, two different modified porous starch microspheres improved the drug loading, slow release and free radical scavenging effects of curcumin.					
37120221	0	66	theme	radical	65:71	arg1	ability					84:90	the free radical scavenging ability	56:90	the free radical scavenging ability	56:90	Modified porous starches loading curcumin and improving the free radical scavenging ability and release properties of curcumin.					
37120221	1	67	theme	starch-curcumin	141:155	arg1	microspheres					157:168	Maize porous starch-curcumin microspheres	128:168	Maize porous starch-curcumin microspheres	128:168	Maize porous starch-curcumin microspheres were prepared by encapsulating curcumin into cross-linked porous starch and oxidized porous starch to investigate the effect of modified porous starch in embedding and protecting curcumin.					
37120221	7	68	theme	release	1466:1472	arg1	effects					1502:1508	the drug loading, slow release and free radical scavenging effects	1443:1508	the drug loading, slow release and free radical scavenging effects of curcumin	1443:1520	To recapitulate, two different modified porous starch microspheres improved the drug loading, slow release and free radical scavenging effects of curcumin.					
37120221	3	69	theme	forces	846:851	arg1	forces					846:851	the major driving forces	828:851	the major driving forces for encapsulation	828:869	The FT-IR results revealed that curcumin was amorphously encapsulated in the composite and hydrogen bond formation between starch and curcumin was one of the major driving forces for encapsulation.					
37120221	3	69	theme	forces	846:851	arg1	one					821:823	one	821:823	one	821:823	The FT-IR results revealed that curcumin was amorphously encapsulated in the composite and hydrogen bond formation between starch and curcumin was one of the major driving forces for encapsulation.					
37120221	4	70	contain	has	952:954	arg1	temperature					921:931	the initial decomposition temperature	895:931	the initial decomposition temperature	895:931	Microspheres increased the initial decomposition temperature of curcumin, which has a protective effect on curcumin.					
37120221	4	70	contain	has	952:954	arg2	effect					969:974	a protective effect	956:974	a protective effect	956:974	Microspheres increased the initial decomposition temperature of curcumin, which has a protective effect on curcumin.					
37120221	8	71	theme	oxidized	1651:1658	arg1	microspheres					1674:1685	the oxidized porous starch microspheres	1647:1685	the oxidized porous starch microspheres	1647:1685	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
37120221	5	72	theme	free	1059:1062	arg1	ability					1072:1078	the scavenging free radical ability	1044:1078	the scavenging free radical ability of porous starch	1044:1095	Modification improved the encapsulation efficiency and the scavenging free radical ability of porous starch.					
37120221	7	73	theme	loading	1452:1458	arg1	effects					1502:1508	the drug loading, slow release and free radical scavenging effects	1443:1508	the drug loading, slow release and free radical scavenging effects of curcumin	1443:1520	To recapitulate, two different modified porous starch microspheres improved the drug loading, slow release and free radical scavenging effects of curcumin.					
37120221	2	74	theme	Thermal	549:555	arg1	stability					557:565	Thermal stability	549:565	Thermal stability	549:565	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	8	75	theme	starch	1667:1672	arg1	microspheres					1674:1685	the oxidized porous starch microspheres	1647:1685	the oxidized porous starch microspheres	1647:1685	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
37120221	0	76	theme	porous	9:14	arg1	starches					16:23	Modified porous starches	0:23	Modified porous starches	0:23	Modified porous starches loading curcumin and improving the free radical scavenging ability and release properties of curcumin.					
37120221	9	77	theme	theoretical	1700:1710	arg1	basis					1734:1738	theoretical significance and data basis	1700:1738	theoretical significance and data basis for the encapsulation of active substances by modified porous starch	1700:1807	It provides theoretical significance and data basis for the encapsulation of active substances by modified porous starch.					
37120221	1	78	theme	porous	307:312	arg1	starch					314:319	modified porous starch	298:319	modified porous starch	298:319	Maize porous starch-curcumin microspheres were prepared by encapsulating curcumin into cross-linked porous starch and oxidized porous starch to investigate the effect of modified porous starch in embedding and protecting curcumin.					
37120221	2	79	dep	morphology	363:372	arg1	The					359:361	The	359:361	The	359:361	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	7	80	theme	porous	1407:1412	arg1	microspheres					1421:1432	two different modified porous starch microspheres	1384:1432	two different modified porous starch microspheres	1384:1432	To recapitulate, two different modified porous starch microspheres improved the drug loading, slow release and free radical scavenging effects of curcumin.					
37120221	6	81	theme	Higuchi	1171:1177	arg1	models					1179:1184	Higuchi models	1171:1184	Higuchi models	1171:1184	The release mechanism of curcumin from microspheres fits first-order and Higuchi models well in gastric and intestinal models, respectively, indicating that encapsulation of curcumin within different porous starches microspheres enables controlled release of curcumin.					
37120221	3	82	theme	FT-IR	678:682	arg1	results					684:690	The FT-IR results	674:690	The FT-IR results	674:690	The FT-IR results revealed that curcumin was amorphously encapsulated in the composite and hydrogen bond formation between starch and curcumin was one of the major driving forces for encapsulation.					
37120221	2	83	theme	electron	450:457	arg1	microscopy					459:468	scanning electron microscopy	441:468	scanning electron microscopy	441:468	The morphology and physicochemical properties of microspheres were analyzed using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction, Zeta/DLS, Thermal stability, and antioxidant activity; the release of curcumin was evaluated with a simulated gastric-intestine model.					
37120221	7	84	theme	different	1388:1396	arg1	microspheres					1421:1432	two different modified porous starch microspheres	1384:1432	two different modified porous starch microspheres	1384:1432	To recapitulate, two different modified porous starch microspheres improved the drug loading, slow release and free radical scavenging effects of curcumin.					
37120221	6	85	theme	intestinal	1206:1215	arg1	models					1217:1222	gastric and intestinal models	1194:1222	gastric and intestinal models	1194:1222	The release mechanism of curcumin from microspheres fits first-order and Higuchi models well in gastric and intestinal models, respectively, indicating that encapsulation of curcumin within different porous starches microspheres enables controlled release of curcumin.					
37120221	1	86	theme	porous	255:260	arg1	starch					262:267	oxidized porous starch	246:267	oxidized porous starch	246:267	Maize porous starch-curcumin microspheres were prepared by encapsulating curcumin into cross-linked porous starch and oxidized porous starch to investigate the effect of modified porous starch in embedding and protecting curcumin.					
37120221	5	87	theme	porous	1083:1088	arg1	starch					1090:1095	porous starch	1083:1095	porous starch	1083:1095	Modification improved the encapsulation efficiency and the scavenging free radical ability of porous starch.					
37120221	6	88	theme	gastric	1194:1200	arg1	models					1217:1222	gastric and intestinal models	1194:1222	gastric and intestinal models	1194:1222	The release mechanism of curcumin from microspheres fits first-order and Higuchi models well in gastric and intestinal models, respectively, indicating that encapsulation of curcumin within different porous starches microspheres enables controlled release of curcumin.					
37120221	8	89	theme	cross-linked	1539:1550	arg1	microspheres					1566:1577	the cross-linked porous starch microspheres	1535:1577	the cross-linked porous starch microspheres	1535:1577	Among them, the cross-linked porous starch microspheres had higher encapsulation and slow release ability for curcumin than the oxidized porous starch microspheres.					
35546344	2	0	theme	central	463:469	arg1	CCD					489:491	CCD	489:491	CCD	489:491	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	2	0	theme	central	463:469	arg1	design					481:486	a central composite design	461:486	a central composite design (CCD)	461:492	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	3	1	theme	suitable	619:626	arg1	combination					628:638	the most suitable combination	610:638	the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance	610:713	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	4	2	theme	functional	810:819	arg1	properties					821:830	improved textural and functional properties	788:830	improved textural and functional properties favourable for consumer acceptance	788:865	The resulting rice fortified with S. platensis and hydrocolloids showed improved textural and functional properties favourable for consumer acceptance.					
35546344	5	3	dep	optimum	885:891	arg1	CMC					909:911	1.0% CMC	904:911	1.0% CMC	904:911	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	5	3	dep	optimum	885:891	arg1	MD					900:901	20.0% MD	894:901	20.0% MD	894:901	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	5	3	dep	optimum	885:891	arg1	powder					936:941	2.0% S. platensis powder	918:941	2.0% S. platensis powder	918:941	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	6	4	theme	/g	1211:1212	arg1	181.5 µg/g					1129:1138	181.5 µg/g	1129:1138	181.5 µg/g	1129:1138	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	4	theme	/g	1211:1212	arg1	TPC					1166:1168	TPC	1166:1168	TPC	1166:1168	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	4	theme	/g	1211:1212	arg1	compound					1156:1163	total phenolic compound	1141:1163	total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g	1141:1212	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	5	5	theme	uncoated	1042:1049	arg1	rice					1051:1054	uncoated rice	1042:1054	uncoated rice	1042:1054	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	6	6	theme	acid	1190:1193	arg1	/g					1211:1212	137.3 µg gallic acid equivalent (GAE)/g	1174:1212	137.3 µg gallic acid equivalent (GAE)/g	1174:1212	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	2	7	theme	carboxymethyl	362:374	arg1	cellulose					376:384	carboxymethyl cellulose	362:384	carboxymethyl cellulose (CMC)	362:390	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	2	7	theme	carboxymethyl	362:374	arg1	polymers					345:352	edible polymers	338:352	edible polymers	338:352	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	2	7	theme	carboxymethyl	362:374	arg1	CMC					387:389	CMC	387:389	CMC	387:389	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	7	8	dep	colour	1374:1379	arg1	the					1370:1372	the	1370:1372	the	1370:1372	It can be stated that masking the colour and flavour of Spirulina with an edible coating could be a healthy alternative to commercial rice and used to fortify cereal products with algae.					
35546344	6	9	theme	equivalent	1195:1204	arg1	/g					1211:1212	137.3 µg gallic acid equivalent (GAE)/g	1174:1212	137.3 µg gallic acid equivalent (GAE)/g	1174:1212	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	4	10	theme	favourable	832:841	arg1	properties					821:830	improved textural and functional properties	788:830	improved textural and functional properties favourable for consumer acceptance	788:865	The resulting rice fortified with S. platensis and hydrocolloids showed improved textural and functional properties favourable for consumer acceptance.					
35546344	6	11	theme	total	1141:1145	arg1	181.5 µg/g					1129:1138	181.5 µg/g	1129:1138	181.5 µg/g	1129:1138	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	11	theme	total	1141:1145	arg1	TPC					1166:1168	TPC	1166:1168	TPC	1166:1168	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	11	theme	total	1141:1145	arg1	compound					1156:1163	total phenolic compound	1141:1163	total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g	1141:1212	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	5	12	theme	%	898:898	arg1	MD					900:901	20.0% MD	894:901	20.0% MD	894:901	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	0	13	theme	physicochemical	83:97	arg1	properties					99:108	physicochemical properties	83:108	physicochemical properties	83:108	Effect of instant jasmine rice coating combining Spirulina with edible polymers on physicochemical properties, textural properties and sensory acceptance.					
35546344	3	14	theme	physicochemical	643:657	arg1	properties					659:668	physicochemical properties	643:668	physicochemical properties	643:668	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	6	15	theme	137.3 µg	1174:1181	arg1	acid					1190:1193	137.3 µg gallic acid	1174:1193	137.3 µg gallic acid equivalent (GAE)/g	1174:1212	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	16	theme	1.4 mg/g	1102:1109	arg1	FRAP					1254:1257	FRAP	1254:1257	FRAP	1254:1257	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	16	theme	1.4 mg/g	1102:1109	arg1	a					1124:1124	chlorophyll a	1112:1124	chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g	1112:1212	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	16	theme	1.4 mg/g	1102:1109	arg1	content					1091:1097	phycocyanin content	1079:1097	phycocyanin content of 1.4 mg/g	1079:1109	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	16	theme	1.4 mg/g	1102:1109	arg1	power					1247:1251	ferric reducing antioxidant power	1219:1251	ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately)	1219:1337	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	7	17	theme	healthy	1440:1446	arg1	alternative					1448:1458	a healthy alternative	1438:1458	a healthy alternative to commercial rice	1438:1477	It can be stated that masking the colour and flavour of Spirulina with an edible coating could be a healthy alternative to commercial rice and used to fortify cereal products with algae.					
35546344	7	18	theme	Spirulina	1396:1404	arg1	colour					1374:1379	colour	1374:1379	colour	1374:1379	It can be stated that masking the colour and flavour of Spirulina with an edible coating could be a healthy alternative to commercial rice and used to fortify cereal products with algae.					
35546344	7	18	theme	Spirulina	1396:1404	arg1	flavour					1385:1391	flavour	1385:1391	flavour	1385:1391	It can be stated that masking the colour and flavour of Spirulina with an edible coating could be a healthy alternative to commercial rice and used to fortify cereal products with algae.					
35546344	0	19	theme	textural	111:118	arg1	properties					120:129	textural properties	111:129	textural properties	111:129	Effect of instant jasmine rice coating combining Spirulina with edible polymers on physicochemical properties, textural properties and sensory acceptance.					
35546344	3	20	theme	properties	659:668	arg1	combination					628:638	the most suitable combination	610:638	the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance	610:713	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	2	21	theme	technical	283:291	arg1	feasibility					293:303	the technical feasibility	279:303	the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD)	279:412	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	1	22	theme	effective	169:177	arg1	Coating					155:161	Coating	155:161	Coating	155:161	Coating is an effective and economic strategy to increase the functional property of food products.					
35546344	1	22	theme	effective	169:177	arg1	strategy					192:199	an effective and economic strategy	166:199	an effective and economic strategy to increase the functional property of food products	166:252	Coating is an effective and economic strategy to increase the functional property of food products.					
35546344	6	23	theme	antioxidant	1235:1245	arg1	FRAP					1254:1257	FRAP	1254:1257	FRAP	1254:1257	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	23	theme	antioxidant	1235:1245	arg1	power					1247:1251	ferric reducing antioxidant power	1219:1251	ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately)	1219:1337	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	24	theme	/g	1284:1285	arg1	FRAP					1254:1257	FRAP	1254:1257	FRAP	1254:1257	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	24	theme	/g	1284:1285	arg1	a					1124:1124	chlorophyll a	1112:1124	chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g	1112:1212	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	24	theme	/g	1284:1285	arg1	content					1091:1097	phycocyanin content	1079:1097	phycocyanin content of 1.4 mg/g	1079:1109	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	24	theme	/g	1284:1285	arg1	power					1247:1251	ferric reducing antioxidant power	1219:1251	ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately)	1219:1337	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	5	25	theme	%	907:907	arg1	CMC					909:911	1.0% CMC	904:911	1.0% CMC	904:911	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	2	26	theme	instant	433:439	arg1	rice					449:452	instant jasmine rice	433:452	instant jasmine rice	433:452	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	6	27	theme	phycocyanin	1079:1089	arg1	content					1091:1097	phycocyanin content	1079:1097	phycocyanin content of 1.4 mg/g	1079:1109	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	4	28	theme	textural	797:804	arg1	properties					821:830	improved textural and functional properties	788:830	improved textural and functional properties favourable for consumer acceptance	788:865	The resulting rice fortified with S. platensis and hydrocolloids showed improved textural and functional properties favourable for consumer acceptance.					
35546344	6	29	theme	ferrous	1270:1276	arg1	/g					1284:1285	3.8 mg ferrous (Fe2+)/g	1263:1285	3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately)	1263:1337	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	3	30	theme	formulations	524:535	arg1	total					497:501	A total	495:501	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v)	495:581	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	1	31	theme	economic	183:190	arg1	Coating					155:161	Coating	155:161	Coating	155:161	Coating is an effective and economic strategy to increase the functional property of food products.					
35546344	1	31	theme	economic	183:190	arg1	strategy					192:199	an effective and economic strategy	166:199	an effective and economic strategy to increase the functional property of food products	166:252	Coating is an effective and economic strategy to increase the functional property of food products.					
35546344	3	32	theme	attributes	680:689	arg1	combination					628:638	the most suitable combination	610:638	the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance	610:713	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	4	33	theme	improved	788:795	arg1	properties					821:830	improved textural and functional properties	788:830	improved textural and functional properties favourable for consumer acceptance	788:865	The resulting rice fortified with S. platensis and hydrocolloids showed improved textural and functional properties favourable for consumer acceptance.					
35546344	6	34	theme	phenolic	1147:1154	arg1	181.5 µg/g					1129:1138	181.5 µg/g	1129:1138	181.5 µg/g	1129:1138	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	34	theme	phenolic	1147:1154	arg1	TPC					1166:1168	TPC	1166:1168	TPC	1166:1168	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	34	theme	phenolic	1147:1154	arg1	compound					1156:1163	total phenolic compound	1141:1163	total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g	1141:1212	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	35	theme	ferric	1219:1224	arg1	FRAP					1254:1257	FRAP	1254:1257	FRAP	1254:1257	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	35	theme	ferric	1219:1224	arg1	power					1247:1251	ferric reducing antioxidant power	1219:1251	ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately)	1219:1337	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	0	36	theme	jasmine	18:24	arg1	coating					31:37	instant jasmine rice coating	10:37	instant jasmine rice coating	10:37	Effect of instant jasmine rice coating combining Spirulina with edible polymers on physicochemical properties, textural properties and sensory acceptance.					
35546344	6	37	with	/g	1284:1285	arg1	acceptability					1300:1312	overall acceptability	1292:1312	overall acceptability of 7.1 (like moderately)	1292:1337	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	38	theme	reducing	1226:1233	arg1	FRAP					1254:1257	FRAP	1254:1257	FRAP	1254:1257	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	38	theme	reducing	1226:1233	arg1	power					1247:1251	ferric reducing antioxidant power	1219:1251	ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately)	1219:1337	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	2	39	theme	Spirulina	315:323	arg1	platensis					325:333	Spirulina platensis	315:333	Spirulina platensis	315:333	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	5	40	theme	%	921:921	arg1	powder					936:941	2.0% S. platensis powder	918:941	2.0% S. platensis powder	918:941	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	0	41	theme	instant	10:16	arg1	coating					31:37	instant jasmine rice coating	10:37	instant jasmine rice coating	10:37	Effect of instant jasmine rice coating combining Spirulina with edible polymers on physicochemical properties, textural properties and sensory acceptance.					
35546344	7	42	theme	commercial	1463:1472	arg1	rice					1474:1477	commercial rice	1463:1477	commercial rice	1463:1477	It can be stated that masking the colour and flavour of Spirulina with an edible coating could be a healthy alternative to commercial rice and used to fortify cereal products with algae.					
35546344	5	43	theme	1.0	904:906	arg1	%					907:907	%	907:907	%	907:907	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	3	44	theme	%	576:576	arg1	w/v					578:580	1-5% w/v	573:580	1-5% w/v	573:580	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	3	44	theme	%	576:576	arg1	MD					569:570	MD	569:570	MD (1-5% w/v)	569:581	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	3	45	theme	coating	516:522	arg1	formulations					524:535	10 edible coating formulations	506:535	10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v)	506:581	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	7	46	theme	cereal	1499:1504	arg1	products					1506:1513	cereal products	1499:1513	cereal products	1499:1513	It can be stated that masking the colour and flavour of Spirulina with an edible coating could be a healthy alternative to commercial rice and used to fortify cereal products with algae.					
35546344	0	47	theme	coating	31:37	arg1	Effect					0:5	Effect	0:5	Effect of instant jasmine rice coating	0:37	Effect of instant jasmine rice coating combining Spirulina with edible polymers on physicochemical properties, textural properties and sensory acceptance.					
35546344	3	48	theme	textural	671:678	arg1	attributes					680:689	textural attributes	671:689	textural attributes	671:689	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	6	49	theme	7.1	1317:1319	arg1	acceptability					1300:1312	overall acceptability	1292:1312	overall acceptability of 7.1 (like moderately)	1292:1337	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	0	50	from	polymers	71:78	arg1	properties					99:108	physicochemical properties	83:108	physicochemical properties	83:108	Effect of instant jasmine rice coating combining Spirulina with edible polymers on physicochemical properties, textural properties and sensory acceptance.					
35546344	0	50	from	polymers	71:78	arg1	properties					120:129	textural properties	111:129	textural properties	111:129	Effect of instant jasmine rice coating combining Spirulina with edible polymers on physicochemical properties, textural properties and sensory acceptance.					
35546344	0	50	from	polymers	71:78	arg1	acceptance					143:152	sensory acceptance	135:152	sensory acceptance	135:152	Effect of instant jasmine rice coating combining Spirulina with edible polymers on physicochemical properties, textural properties and sensory acceptance.					
35546344	4	51	theme	S.	750:751	arg1	platensis					753:761	S. platensis	750:761	S. platensis	750:761	The resulting rice fortified with S. platensis and hydrocolloids showed improved textural and functional properties favourable for consumer acceptance.					
35546344	6	52	theme	chlorophyll	1112:1122	arg1	a					1124:1124	chlorophyll a	1112:1124	chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g	1112:1212	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	0	53	theme	rice	26:29	arg1	coating					31:37	instant jasmine rice coating	10:37	instant jasmine rice coating	10:37	Effect of instant jasmine rice coating combining Spirulina with edible polymers on physicochemical properties, textural properties and sensory acceptance.					
35546344	3	54	theme	acceptance	704:713	arg1	combination					628:638	the most suitable combination	610:638	the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance	610:713	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	0	55	theme	sensory	135:141	arg1	acceptance					143:152	sensory acceptance	135:152	sensory acceptance	135:152	Effect of instant jasmine rice coating combining Spirulina with edible polymers on physicochemical properties, textural properties and sensory acceptance.					
35546344	3	56	theme	%	558:558	arg1	CMC					548:550	CMC	548:550	CMC (10-30% w/v)	548:563	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	3	56	theme	%	558:558	arg1	w/v					560:562	10-30% w/v	553:562	10-30% w/v	553:562	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	1	57	theme	functional	217:226	arg1	property					228:235	the functional property	213:235	the functional property of food products	213:252	Coating is an effective and economic strategy to increase the functional property of food products.					
35546344	2	58	theme	rice	449:452	arg1	coating					422:428	the coating	418:428	the coating of instant jasmine rice	418:452	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	4	59	theme	consumer	847:854	arg1	acceptance					856:865	consumer acceptance	847:865	consumer acceptance	847:865	The resulting rice fortified with S. platensis and hydrocolloids showed improved textural and functional properties favourable for consumer acceptance.					
35546344	5	60	theme	textural	999:1006	arg1	properties					1008:1017	textural properties	999:1017	textural properties	999:1017	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	5	61	theme	platensis	926:934	arg1	powder					936:941	2.0% S. platensis powder	918:941	2.0% S. platensis powder	918:941	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	6	62	dep	7.1	1317:1319	arg1	moderately					1327:1336	moderately	1327:1336	moderately	1327:1336	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	2	63	theme	jasmine	441:447	arg1	rice					449:452	instant jasmine rice	433:452	instant jasmine rice	433:452	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	6	64	theme	Fe2+	1279:1282	arg1	/g					1284:1285	3.8 mg ferrous (Fe2+)/g	1263:1285	3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately)	1263:1337	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	65	theme	gallic	1183:1188	arg1	acid					1190:1193	137.3 µg gallic acid	1174:1193	137.3 µg gallic acid equivalent (GAE)/g	1174:1212	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	3	66	theme	sensory	696:702	arg1	acceptance					704:713	sensory acceptance	696:713	sensory acceptance	696:713	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	6	67	theme	GAE	1207:1209	arg1	/g					1211:1212	137.3 µg gallic acid equivalent (GAE)/g	1174:1212	137.3 µg gallic acid equivalent (GAE)/g	1174:1212	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	5	68	theme	20.0	894:897	arg1	%					898:898	%	898:898	%	898:898	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	4	69	theme	resulting	720:728	arg1	rice					730:733	The resulting rice	716:733	The resulting rice fortified with S. platensis and hydrocolloids	716:779	The resulting rice fortified with S. platensis and hydrocolloids showed improved textural and functional properties favourable for consumer acceptance.					
35546344	5	70	theme	S.	923:924	arg1	powder					936:941	2.0% S. platensis powder	918:941	2.0% S. platensis powder	918:941	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	3	71	theme	edible	509:514	arg1	formulations					524:535	10 edible coating formulations	506:535	10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v)	506:581	A total of 10 edible coating formulations comprising CMC (10-30% w/v) and MD (1-5% w/v) were evaluated to optimize the most suitable combination of physicochemical properties, textural attributes, and sensory acceptance.					
35546344	6	72	theme	overall	1292:1298	arg1	acceptability					1300:1312	overall acceptability	1292:1312	overall acceptability of 7.1 (like moderately)	1292:1337	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	2	73	theme	edible	338:343	arg1	maltodextrin					396:407	maltodextrin	396:407	maltodextrin (MD)	396:412	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	2	73	theme	edible	338:343	arg1	cellulose					376:384	carboxymethyl cellulose	362:384	carboxymethyl cellulose (CMC)	362:390	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	2	73	theme	edible	338:343	arg1	polymers					345:352	edible polymers	338:352	edible polymers	338:352	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	7	74	theme	edible	1414:1419	arg1	coating					1421:1427	an edible coating	1411:1427	an edible coating	1411:1427	It can be stated that masking the colour and flavour of Spirulina with an edible coating could be a healthy alternative to commercial rice and used to fortify cereal products with algae.					
35546344	0	75	theme	edible	64:69	arg1	polymers					71:78	edible polymers	64:78	edible polymers on physicochemical properties, textural properties and sensory acceptance	64:152	Effect of instant jasmine rice coating combining Spirulina with edible polymers on physicochemical properties, textural properties and sensory acceptance.					
35546344	5	76	theme	physicochemical	958:972	arg1	properties					974:983	the physicochemical properties	954:983	the physicochemical properties	954:983	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	6	77	theme	3.8 mg	1263:1268	arg1	/g					1284:1285	3.8 mg ferrous (Fe2+)/g	1263:1285	3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately)	1263:1337	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	1	78	theme	food	240:243	arg1	products					245:252	food products	240:252	food products	240:252	Coating is an effective and economic strategy to increase the functional property of food products.					
35546344	2	79	theme	composite	471:479	arg1	CCD					489:491	CCD	489:491	CCD	489:491	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	2	79	theme	composite	471:479	arg1	design					481:486	a central composite design	461:486	a central composite design (CCD)	461:492	This study investigated the technical feasibility of adding Spirulina platensis to edible polymers, namely carboxymethyl cellulose (CMC) and maltodextrin (MD), in the coating of instant jasmine rice, using a central composite design (CCD).					
35546344	5	80	theme	2.0	918:920	arg1	%					921:921	%	921:921	%	921:921	Among these, the optimum (20.0% MD, 1.0% CMC, and 2.0% S. platensis powder) increased the physicochemical properties and decreased textural properties compared with those of uncoated rice.					
35546344	6	81	theme	181.5 µg/g	1129:1138	arg1	FRAP					1254:1257	FRAP	1254:1257	FRAP	1254:1257	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	81	theme	181.5 µg/g	1129:1138	arg1	a					1124:1124	chlorophyll a	1112:1124	chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g	1112:1212	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	81	theme	181.5 µg/g	1129:1138	arg1	content					1091:1097	phycocyanin content	1079:1097	phycocyanin content of 1.4 mg/g	1079:1109	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	6	81	theme	181.5 µg/g	1129:1138	arg1	power					1247:1251	ferric reducing antioxidant power	1219:1251	ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately)	1219:1337	This condition showed phycocyanin content of 1.4 mg/g, chlorophyll a of 181.5 µg/g, total phenolic compound (TPC) of 137.3 µg gallic acid equivalent (GAE)/g, and ferric reducing antioxidant power (FRAP) of 3.8 mg ferrous (Fe2+)/g with overall acceptability of 7.1 (like moderately).					
35546344	1	82	theme	products	245:252	arg1	property					228:235	the functional property	213:235	the functional property of food products	213:252	Coating is an effective and economic strategy to increase the functional property of food products.					
37032226	9	0	theme	IFN-γ	1325:1329	arg1	levels					1315:1320	The levels	1311:1320	The levels of IFN-γ	1311:1329	The levels of IFN-γ were more than twice those of the commercial vaccine at 7 days post the immunization.					
37032226	9	0	theme	IFN-γ	1325:1329	arg1	more					1336:1339	more	1336:1339	more	1336:1339	The levels of IFN-γ were more than twice those of the commercial vaccine at 7 days post the immunization.					
37032226	10	1	theme	N-2-HACC-Al	1421:1431	arg1	adjuvant					1472:1479	adjuvant	1472:1479	adjuvant	1472:1479	The N-2-HACC-Al NPs could be used as an efficient nano adjuvant to enhance the effectiveness of vaccine and have immense application potential.					
37032226	10	1	theme	N-2-HACC-Al	1421:1431	arg1	NPs					1433:1435	The N-2-HACC-Al NPs	1417:1435	The N-2-HACC-Al NPs	1417:1435	The N-2-HACC-Al NPs could be used as an efficient nano adjuvant to enhance the effectiveness of vaccine and have immense application potential.					
37032226	6	2	theme	combined	912:919	arg1	vaccine					933:939	the combined inactivated vaccine	908:939	the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI)	908:1000	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	6	2	theme	combined	912:919	arg1	vaccine					1045:1051	a vaccine adjuvant	1043:1060	a vaccine adjuvant	1043:1060	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	2	3	theme	humoral	399:405	arg1	responses					427:435	humoral and cellular immune responses	399:435	humoral and cellular immune responses of vaccines	399:447	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	1	4	theme	immune	268:273	arg1	response					275:282	cellular immune response	259:282	cellular immune response	259:282	Aluminum adjuvant is a typical adjuvant that can promote humoral immune response, but it lacks the ability to effectively induce cellular immune response.					
37032226	2	5	theme	vaccines	440:447	arg1	responses					427:435	humoral and cellular immune responses	399:435	humoral and cellular immune responses of vaccines	399:447	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	7	6	theme	chicken	1135:1141	arg1	immunization					1151:1162	chicken in vivo immunization	1135:1162	chicken in vivo immunization	1135:1162	The immune effect of the vaccine (N-2-HACC-Al/NDV-AIV) was evaluated by chicken in vivo immunization.					
37032226	1	7	theme	humoral	187:193	arg1	response					202:209	humoral immune response	187:209	humoral immune response	187:209	Aluminum adjuvant is a typical adjuvant that can promote humoral immune response, but it lacks the ability to effectively induce cellular immune response.					
37032226	8	8	theme	IFN-γ	1223:1227	arg1	levels					1192:1197	higher levels	1185:1197	higher levels of serum IgG, IL-4, and IFN-γ	1185:1227	The vaccine induced higher levels of serum IgG, IL-4, and IFN-γ than those of the commercial combined inactivated vaccine against ND and H9N2 AI.					
37032226	6	9	theme	Newcastle	949:957	arg1	disease					959:965	Newcastle disease	949:965	Newcastle disease (ND)	949:970	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	6	9	theme	Newcastle	949:957	arg1	ND					968:969	ND	968:969	ND	968:969	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	2	10	theme	ammonium	331:338	arg1	chloride					340:347	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride	285:347	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs)	285:385	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	2	11	theme	chloride	340:347	arg1	chitosan					349:356	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan	285:356	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs)	285:385	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	5	12	theme	good	761:764	arg1	stability					774:782	good thermal stability	761:782	good thermal stability	761:782	The N-2-HACC-Al NPs have good thermal stability and biodegradability and lower cytotoxicity.					
37032226	2	13	theme	trimethyl	321:329	arg1	chloride					340:347	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride	285:347	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs)	285:385	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	3	14	theme	adjuvant	526:533	arg1	NPs					547:549	the composite nano adjuvant N-2-HACC-Al NPs	507:549	the composite nano adjuvant N-2-HACC-Al NPs	507:549	To enable aluminum adjuvant to induce cellular immunity, the composite nano adjuvant N-2-HACC-Al NPs were synthesized by the N-2-HACC and aluminum sulfate (Al2(SO4)3).					
37032226	5	15	theme	thermal	766:772	arg1	stability					774:782	good thermal stability	761:782	good thermal stability	761:782	The N-2-HACC-Al NPs have good thermal stability and biodegradability and lower cytotoxicity.					
37032226	3	16	dep	N-2-HACC	575:582	arg1	the					571:573	the	571:573	the	571:573	To enable aluminum adjuvant to induce cellular immunity, the composite nano adjuvant N-2-HACC-Al NPs were synthesized by the N-2-HACC and aluminum sulfate (Al2(SO4)3).					
37032226	7	17	dep	in	1143:1144	arg1	vivo					1146:1149	vivo	1146:1149	vivo	1146:1149	The immune effect of the vaccine (N-2-HACC-Al/NDV-AIV) was evaluated by chicken in vivo immunization.					
37032226	4	18	theme	zeta	640:643	arg1	potential					645:653	zeta potential	640:653	zeta potential	640:653	The particle size and zeta potential of the N-2-HACC-Al NPs were 300.70 ± 24.90 nm and 32.28 ± 0.52 mV, respectively.					
37032226	2	19	theme	N-2-Hydroxypropyl	303:319	arg1	chloride					340:347	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride	285:347	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs)	285:385	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	8	20	theme	serum	1202:1206	arg1	IgG					1208:1210	serum IgG	1202:1210	serum IgG	1202:1210	The vaccine induced higher levels of serum IgG, IL-4, and IFN-γ than those of the commercial combined inactivated vaccine against ND and H9N2 AI.					
37032226	1	21	theme	immune	195:200	arg1	response					202:209	humoral immune response	187:209	humoral immune response	187:209	Aluminum adjuvant is a typical adjuvant that can promote humoral immune response, but it lacks the ability to effectively induce cellular immune response.					
37032226	6	22	theme	inactivated	921:931	arg1	vaccine					933:939	the combined inactivated vaccine	908:939	the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI)	908:1000	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	6	22	theme	inactivated	921:931	arg1	vaccine					1045:1051	a vaccine adjuvant	1043:1060	a vaccine adjuvant	1043:1060	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	8	23	theme	IL-4	1213:1216	arg1	levels					1192:1197	higher levels	1185:1197	higher levels of serum IgG, IL-4, and IFN-γ	1185:1227	The vaccine induced higher levels of serum IgG, IL-4, and IFN-γ than those of the commercial combined inactivated vaccine against ND and H9N2 AI.					
37032226	2	24	theme	water-soluble	289:301	arg1	chloride					340:347	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride	285:347	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs)	285:385	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	3	25	theme	cellular	488:495	arg1	immunity					497:504	cellular immunity	488:504	cellular immunity	488:504	To enable aluminum adjuvant to induce cellular immunity, the composite nano adjuvant N-2-HACC-Al NPs were synthesized by the N-2-HACC and aluminum sulfate (Al2(SO4)3).					
37032226	0	26	theme	quaternized	11:21	arg1	formulation					79:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation promotes immune responses in chickens.					
37032226	5	27	contain	have	756:759	arg2	biodegradability					788:803	biodegradability	788:803	biodegradability	788:803	The N-2-HACC-Al NPs have good thermal stability and biodegradability and lower cytotoxicity.					
37032226	5	27	contain	have	756:759	arg2	stability					774:782	good thermal stability	761:782	good thermal stability	761:782	The N-2-HACC-Al NPs have good thermal stability and biodegradability and lower cytotoxicity.					
37032226	5	27	contain	have	756:759	arg1	NPs					752:754	The N-2-HACC-Al NPs	736:754	The N-2-HACC-Al NPs	736:754	The N-2-HACC-Al NPs have good thermal stability and biodegradability and lower cytotoxicity.					
37032226	7	28	theme	vaccine	1088:1094	arg1	effect					1074:1079	The immune effect	1063:1079	The immune effect of the vaccine (N-2-HACC-Al/NDV-AIV)	1063:1116	The immune effect of the vaccine (N-2-HACC-Al/NDV-AIV) was evaluated by chicken in vivo immunization.					
37032226	10	29	contain	have	1525:1528	arg2	potential					1550:1558	immense application potential	1530:1558	immense application potential	1530:1558	The N-2-HACC-Al NPs could be used as an efficient nano adjuvant to enhance the effectiveness of vaccine and have immense application potential.					
37032226	10	29	contain	have	1525:1528	arg1	adjuvant					1472:1479	adjuvant	1472:1479	adjuvant	1472:1479	The N-2-HACC-Al NPs could be used as an efficient nano adjuvant to enhance the effectiveness of vaccine and have immense application potential.					
37032226	10	29	contain	have	1525:1528	arg1	NPs					1433:1435	The N-2-HACC-Al NPs	1417:1435	The N-2-HACC-Al NPs	1417:1435	The N-2-HACC-Al NPs could be used as an efficient nano adjuvant to enhance the effectiveness of vaccine and have immense application potential.					
37032226	3	30	theme	adjuvant	469:476	arg1	aluminum					460:467	aluminum adjuvant	460:476	aluminum adjuvant	460:476	To enable aluminum adjuvant to induce cellular immunity, the composite nano adjuvant N-2-HACC-Al NPs were synthesized by the N-2-HACC and aluminum sulfate (Al2(SO4)3).					
37032226	0	31	theme	Adjuvanted	0:9	arg1	formulation					79:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation promotes immune responses in chickens.					
37032226	4	32	theme	NPs	674:676	arg1	size					631:634	particle size	622:634	particle size	622:634	The particle size and zeta potential of the N-2-HACC-Al NPs were 300.70 ± 24.90 nm and 32.28 ± 0.52 mV, respectively.					
37032226	4	32	theme	NPs	674:676	arg1	potential					645:653	zeta potential	640:653	zeta potential	640:653	The particle size and zeta potential of the N-2-HACC-Al NPs were 300.70 ± 24.90 nm and 32.28 ± 0.52 mV, respectively.					
37032226	2	33	theme	immune	420:425	arg1	responses					427:435	humoral and cellular immune responses	399:435	humoral and cellular immune responses of vaccines	399:447	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	0	34	theme	composite	32:40	arg1	formulation					79:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation promotes immune responses in chickens.					
37032226	2	35	theme	chitosan	349:356	arg1	nanoparticles					358:370	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles	285:370	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs)	285:385	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	2	35	theme	chitosan	349:356	arg1	NPs					382:384	N-2-HACC NPs	373:384	N-2-HACC NPs	373:384	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	8	36	theme	commercial	1247:1256	arg1	vaccine					1279:1285	the commercial combined inactivated vaccine	1243:1285	the commercial combined inactivated vaccine against ND and H9N2 AI	1243:1308	The vaccine induced higher levels of serum IgG, IL-4, and IFN-γ than those of the commercial combined inactivated vaccine against ND and H9N2 AI.					
37032226	0	37	theme	chitosan	23:30	arg1	formulation					79:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation promotes immune responses in chickens.					
37032226	7	38	theme	immune	1067:1072	arg1	effect					1074:1079	The immune effect	1063:1079	The immune effect of the vaccine (N-2-HACC-Al/NDV-AIV)	1063:1116	The immune effect of the vaccine (N-2-HACC-Al/NDV-AIV) was evaluated by chicken in vivo immunization.					
37032226	1	39	theme	adjuvant	139:146	arg1	adjuvant					161:168	adjuvant	161:168	adjuvant	161:168	Aluminum adjuvant is a typical adjuvant that can promote humoral immune response, but it lacks the ability to effectively induce cellular immune response.					
37032226	1	39	theme	adjuvant	139:146	arg1	Aluminum					130:137	Aluminum adjuvant	130:146	Aluminum adjuvant	130:146	Aluminum adjuvant is a typical adjuvant that can promote humoral immune response, but it lacks the ability to effectively induce cellular immune response.					
37032226	10	40	used	used	1446:1449	arg2	NPs					1433:1435	The N-2-HACC-Al NPs	1417:1435	The N-2-HACC-Al NPs	1417:1435	The N-2-HACC-Al NPs could be used as an efficient nano adjuvant to enhance the effectiveness of vaccine and have immense application potential.					
37032226	10	40	used	used	1446:1449	arg2	adjuvant					1472:1479	adjuvant	1472:1479	adjuvant	1472:1479	The N-2-HACC-Al NPs could be used as an efficient nano adjuvant to enhance the effectiveness of vaccine and have immense application potential.					
37032226	3	41	theme	composite	511:519	arg1	NPs					547:549	the composite nano adjuvant N-2-HACC-Al NPs	507:549	the composite nano adjuvant N-2-HACC-Al NPs	507:549	To enable aluminum adjuvant to induce cellular immunity, the composite nano adjuvant N-2-HACC-Al NPs were synthesized by the N-2-HACC and aluminum sulfate (Al2(SO4)3).					
37032226	0	42	theme	nanoparticles-based	51:69	arg1	formulation					79:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation promotes immune responses in chickens.					
37032226	5	43	theme	N-2-HACC-Al	740:750	arg1	NPs					752:754	The N-2-HACC-Al NPs	736:754	The N-2-HACC-Al NPs	736:754	The N-2-HACC-Al NPs have good thermal stability and biodegradability and lower cytotoxicity.					
37032226	8	44	theme	inactivated	1267:1277	arg1	vaccine					1279:1285	the commercial combined inactivated vaccine	1243:1285	the commercial combined inactivated vaccine against ND and H9N2 AI	1243:1308	The vaccine induced higher levels of serum IgG, IL-4, and IFN-γ than those of the commercial combined inactivated vaccine against ND and H9N2 AI.					
37032226	6	45	theme	adjuvant	1053:1060	arg1	vaccine					933:939	the combined inactivated vaccine	908:939	the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI)	908:1000	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	6	45	theme	adjuvant	1053:1060	arg1	vaccine					1045:1051	a vaccine adjuvant	1043:1060	a vaccine adjuvant	1043:1060	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	0	46	theme	aluminum	42:49	arg1	formulation					79:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation promotes immune responses in chickens.					
37032226	6	47	theme	avian	981:985	arg1	AI					998:999	AI	998:999	AI	998:999	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	6	47	theme	avian	981:985	arg1	influenza					987:995	H9N2 avian influenza	976:995	H9N2 avian influenza (AI)	976:1000	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	5	48	theme	lower	809:813	arg1	cytotoxicity					815:826	lower cytotoxicity	809:826	lower cytotoxicity	809:826	The N-2-HACC-Al NPs have good thermal stability and biodegradability and lower cytotoxicity.					
37032226	6	49	theme	adjuvant	898:905	arg1	nano					893:896	the composite nano adjuvant	879:905	the composite nano adjuvant	879:905	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	8	50	theme	IgG	1208:1210	arg1	levels					1192:1197	higher levels	1185:1197	higher levels of serum IgG, IL-4, and IFN-γ	1185:1227	The vaccine induced higher levels of serum IgG, IL-4, and IFN-γ than those of the commercial combined inactivated vaccine against ND and H9N2 AI.					
37032226	8	51	theme	ND	1295:1296	arg1	AI					1307:1308	ND and H9N2 AI	1295:1308	ND and H9N2 AI	1295:1308	The vaccine induced higher levels of serum IgG, IL-4, and IFN-γ than those of the commercial combined inactivated vaccine against ND and H9N2 AI.					
37032226	7	52	theme	in	1143:1144	arg1	immunization					1151:1162	chicken in vivo immunization	1135:1162	chicken in vivo immunization	1135:1162	The immune effect of the vaccine (N-2-HACC-Al/NDV-AIV) was evaluated by chicken in vivo immunization.					
37032226	6	53	theme	H9N2	976:979	arg1	AI					998:999	AI	998:999	AI	998:999	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	6	53	theme	H9N2	976:979	arg1	influenza					987:995	H9N2 avian influenza	976:995	H9N2 avian influenza (AI)	976:1000	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	6	54	theme	nano	893:896	arg1	immunogenicity					861:874	the immunogenicity	857:874	the immunogenicity of the composite nano adjuvant	857:905	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	3	55	theme	N-2-HACC-Al	535:545	arg1	NPs					547:549	the composite nano adjuvant N-2-HACC-Al NPs	507:549	the composite nano adjuvant N-2-HACC-Al NPs	507:549	To enable aluminum adjuvant to induce cellular immunity, the composite nano adjuvant N-2-HACC-Al NPs were synthesized by the N-2-HACC and aluminum sulfate (Al2(SO4)3).					
37032226	0	56	theme	vaccine	71:77	arg1	formulation					79:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation	0:89	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation promotes immune responses in chickens.					
37032226	6	57	theme	composite	883:891	arg1	nano					893:896	the composite nano adjuvant	879:905	the composite nano adjuvant	879:905	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	4	58	dep	size	631:634	arg1	The					618:620	The	618:620	The	618:620	The particle size and zeta potential of the N-2-HACC-Al NPs were 300.70 ± 24.90 nm and 32.28 ± 0.52 mV, respectively.					
37032226	10	59	theme	application	1538:1548	arg1	potential					1550:1558	immense application potential	1530:1558	immense application potential	1530:1558	The N-2-HACC-Al NPs could be used as an efficient nano adjuvant to enhance the effectiveness of vaccine and have immense application potential.					
37032226	3	60	theme	nano	521:524	arg1	NPs					547:549	the composite nano adjuvant N-2-HACC-Al NPs	507:549	the composite nano adjuvant N-2-HACC-Al NPs	507:549	To enable aluminum adjuvant to induce cellular immunity, the composite nano adjuvant N-2-HACC-Al NPs were synthesized by the N-2-HACC and aluminum sulfate (Al2(SO4)3).					
37032226	10	61	theme	immense	1530:1536	arg1	potential					1550:1558	immense application potential	1530:1558	immense application potential	1530:1558	The N-2-HACC-Al NPs could be used as an efficient nano adjuvant to enhance the effectiveness of vaccine and have immense application potential.					
37032226	4	62	theme	particle	622:629	arg1	size					631:634	particle size	622:634	particle size	622:634	The particle size and zeta potential of the N-2-HACC-Al NPs were 300.70 ± 24.90 nm and 32.28 ± 0.52 mV, respectively.					
37032226	3	63	theme	aluminum	588:595	arg1	sulfate					597:603	aluminum sulfate	588:603	aluminum sulfate	588:603	To enable aluminum adjuvant to induce cellular immunity, the composite nano adjuvant N-2-HACC-Al NPs were synthesized by the N-2-HACC and aluminum sulfate (Al2(SO4)3).					
37032226	2	64	theme	N-2-HACC	373:380	arg1	nanoparticles					358:370	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles	285:370	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs)	285:385	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	2	64	theme	N-2-HACC	373:380	arg1	NPs					382:384	N-2-HACC NPs	373:384	N-2-HACC NPs	373:384	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	0	65	theme	immune	100:105	arg1	responses					107:115	immune responses	100:115	immune responses	100:115	Adjuvanted quaternized chitosan composite aluminum nanoparticles-based vaccine formulation promotes immune responses in chickens.					
37032226	8	66	theme	combined	1258:1265	arg1	vaccine					1279:1285	the commercial combined inactivated vaccine	1243:1285	the commercial combined inactivated vaccine against ND and H9N2 AI	1243:1308	The vaccine induced higher levels of serum IgG, IL-4, and IFN-γ than those of the commercial combined inactivated vaccine against ND and H9N2 AI.					
37032226	8	67	theme	higher	1185:1190	arg1	levels					1192:1197	higher levels	1185:1197	higher levels of serum IgG, IL-4, and IFN-γ	1185:1227	The vaccine induced higher levels of serum IgG, IL-4, and IFN-γ than those of the commercial combined inactivated vaccine against ND and H9N2 AI.					
37032226	2	68	theme	cellular	411:418	arg1	responses					427:435	humoral and cellular immune responses	399:435	humoral and cellular immune responses of vaccines	399:447	The water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles (N-2-HACC NPs) can enhance humoral and cellular immune responses of vaccines.					
37032226	4	69	theme	N-2-HACC-Al	662:672	arg1	NPs					674:676	the N-2-HACC-Al NPs	658:676	the N-2-HACC-Al NPs	658:676	The particle size and zeta potential of the N-2-HACC-Al NPs were 300.70 ± 24.90 nm and 32.28 ± 0.52 mV, respectively.					
37032226	9	70	theme	commercial	1365:1374	arg1	vaccine					1376:1382	the commercial vaccine	1361:1382	the commercial vaccine	1361:1382	The levels of IFN-γ were more than twice those of the commercial vaccine at 7 days post the immunization.					
37032226	10	71	theme	vaccine	1513:1519	arg1	effectiveness					1496:1508	the effectiveness	1492:1508	the effectiveness of vaccine	1492:1519	The N-2-HACC-Al NPs could be used as an efficient nano adjuvant to enhance the effectiveness of vaccine and have immense application potential.					
37032226	6	72	theme	N-2-HACC-Al	1024:1034	arg1	NPs					1036:1038	the N-2-HACC-Al NPs	1020:1038	the N-2-HACC-Al NPs	1020:1038	In addition, to investigate the immunogenicity of the composite nano adjuvant, the combined inactivated vaccine against Newcastle disease (ND) and H9N2 avian influenza (AI) was prepared with the N-2-HACC-Al NPs as a vaccine adjuvant.					
37032226	8	73	theme	H9N2	1302:1305	arg1	AI					1307:1308	ND and H9N2 AI	1295:1308	ND and H9N2 AI	1295:1308	The vaccine induced higher levels of serum IgG, IL-4, and IFN-γ than those of the commercial combined inactivated vaccine against ND and H9N2 AI.					
37032226	1	74	theme	cellular	259:266	arg1	response					275:282	cellular immune response	259:282	cellular immune response	259:282	Aluminum adjuvant is a typical adjuvant that can promote humoral immune response, but it lacks the ability to effectively induce cellular immune response.					
36592600	0	0	theme	ion	91:93	arg1	pairs					95:99	three fragment ion pairs	76:99	three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine	76:155	Rapid identification and relative quantification of disaccharide isomers by three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine.					
36592600	2	1	theme	quantified	378:387	arg1	PMP-disaccharides					400:416	and relatively quantified protonated PMP-disaccharides	363:416	PMP-disaccharides	400:416	Herein, a novel ESI-MS/MS based strategy was established to discriminate and relatively quantified protonated PMP-disaccharides with different composition and glycosidic bond.					
36592600	0	2	theme	fragment	82:89	arg1	pairs					95:99	three fragment ion pairs	76:99	three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine	76:155	Rapid identification and relative quantification of disaccharide isomers by three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine.					
36592600	6	3	from	easy	1122:1125	arg1	general					1097:1103	general	1097:1103	general	1097:1103	In general, this method was easy to operation and effective for rapid differentiation and quantification of isomeric disaccharides in complex matrices.					
36592600	1	4	theme	Small	158:162	arg1	differences					175:185	Small structural differences	158:185	Small structural differences	158:185	Small structural differences bring great difficulties on carbohydrates identification, especially in terms of their quantification.					
36592600	2	5	theme	novel	300:304	arg1	strategy					322:329	a novel ESI-MS/MS based strategy	298:329	a novel ESI-MS/MS based strategy	298:329	Herein, a novel ESI-MS/MS based strategy was established to discriminate and relatively quantified protonated PMP-disaccharides with different composition and glycosidic bond.					
36592600	3	6	theme	fragment	643:650	arg1	patterns					652:659	diagnostic fragment patterns	632:659	diagnostic fragment patterns for isomers differentiation	632:687	Interestingly, protonated PMP labeled-disaccharides provided abundant fragment ions arising from cross-ring cleavage and glycosidic bond cleavage, which could afford diagnostic fragment patterns for isomers differentiation in combination of statistical analysis.					
36592600	1	7	theme	quantification	274:287	arg1	terms					259:263	terms	259:263	terms of their quantification	259:287	Small structural differences bring great difficulties on carbohydrates identification, especially in terms of their quantification.					
36592600	6	8	from	differentiation	1164:1178	arg1	matrices					1236:1243	complex matrices	1228:1243	complex matrices	1228:1243	In general, this method was easy to operation and effective for rapid differentiation and quantification of isomeric disaccharides in complex matrices.					
36592600	5	9	theme	wine	1008:1011	arg1	discrimination					978:991	the discrimination	974:991	the discrimination of yellow rice wine	974:1011	Ultimately, this method was applied for the discrimination of yellow rice wine, and then the relative content of maltose and isomaltose were confirmed as well.					
36592600	4	10	theme	relative	759:766	arg1	intensity					768:776	the relative intensity	755:776	the relative intensity ratios (RIR) of three ion pairs	755:808	It was worth to note that the relative intensity ratios (RIR) of three ion pairs could completely discriminate 16 disaccharides, and subsequently used to relatively quantified isomers in a binary mixture.					
36592600	1	11	theme	structural	164:173	arg1	differences					175:185	Small structural differences	158:185	Small structural differences	158:185	Small structural differences bring great difficulties on carbohydrates identification, especially in terms of their quantification.					
36592600	4	12	theme	binary	918:923	arg1	mixture					925:931	a binary mixture	916:931	a binary mixture	916:931	It was worth to note that the relative intensity ratios (RIR) of three ion pairs could completely discriminate 16 disaccharides, and subsequently used to relatively quantified isomers in a binary mixture.					
36592600	5	13	theme	maltose	1047:1053	arg1	content					1036:1042	then the relative content	1018:1042	then the relative content of maltose and isomaltose	1018:1068	Ultimately, this method was applied for the discrimination of yellow rice wine, and then the relative content of maltose and isomaltose were confirmed as well.					
36592600	5	13	theme	maltose	1047:1053	arg1	well					1088:1091	well	1088:1091	well	1088:1091	Ultimately, this method was applied for the discrimination of yellow rice wine, and then the relative content of maltose and isomaltose were confirmed as well.					
36592600	6	14	from	general	1097:1103	arg1	easy					1122:1125	easy	1122:1125	easy	1122:1125	In general, this method was easy to operation and effective for rapid differentiation and quantification of isomeric disaccharides in complex matrices.					
36592600	6	15	theme	complex	1228:1234	arg1	matrices					1236:1243	complex matrices	1228:1243	complex matrices	1228:1243	In general, this method was easy to operation and effective for rapid differentiation and quantification of isomeric disaccharides in complex matrices.					
36592600	2	16	theme	different	423:431	arg1	composition					433:443	different composition	423:443	different composition	423:443	Herein, a novel ESI-MS/MS based strategy was established to discriminate and relatively quantified protonated PMP-disaccharides with different composition and glycosidic bond.					
36592600	0	17	theme	Rapid	0:4	arg1	identification					6:19	Rapid identification	0:19	Rapid identification	0:19	Rapid identification and relative quantification of disaccharide isomers by three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine.					
36592600	4	18	theme	pairs	804:808	arg1	RIR					786:788	RIR	786:788	RIR	786:788	It was worth to note that the relative intensity ratios (RIR) of three ion pairs could completely discriminate 16 disaccharides, and subsequently used to relatively quantified isomers in a binary mixture.					
36592600	4	18	theme	pairs	804:808	arg1	ratios					778:783	the relative intensity ratios	755:783	the relative intensity ratios (RIR) of three ion pairs	755:808	It was worth to note that the relative intensity ratios (RIR) of three ion pairs could completely discriminate 16 disaccharides, and subsequently used to relatively quantified isomers in a binary mixture.					
36592600	6	19	from	disaccharides	1211:1223	arg1	matrices					1236:1243	complex matrices	1228:1243	complex matrices	1228:1243	In general, this method was easy to operation and effective for rapid differentiation and quantification of isomeric disaccharides in complex matrices.					
36592600	1	20	theme	great	193:197	arg1	difficulties					199:210	great difficulties	193:210	great difficulties	193:210	Small structural differences bring great difficulties on carbohydrates identification, especially in terms of their quantification.					
36592600	0	21	theme	relative	25:32	arg1	quantification					34:47	relative quantification	25:47	relative quantification	25:47	Rapid identification and relative quantification of disaccharide isomers by three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine.					
36592600	6	22	theme	isomeric	1202:1209	arg1	disaccharides					1211:1223	isomeric disaccharides	1202:1223	isomeric disaccharides in complex matrices	1202:1243	In general, this method was easy to operation and effective for rapid differentiation and quantification of isomeric disaccharides in complex matrices.					
36592600	2	23	theme	based	316:320	arg1	strategy					322:329	a novel ESI-MS/MS based strategy	298:329	a novel ESI-MS/MS based strategy	298:329	Herein, a novel ESI-MS/MS based strategy was established to discriminate and relatively quantified protonated PMP-disaccharides with different composition and glycosidic bond.					
36592600	3	24	theme	fragment	536:543	arg1	ions					545:548	abundant fragment ions	527:548	abundant fragment ions	527:548	Interestingly, protonated PMP labeled-disaccharides provided abundant fragment ions arising from cross-ring cleavage and glycosidic bond cleavage, which could afford diagnostic fragment patterns for isomers differentiation in combination of statistical analysis.					
36592600	2	25	theme	ESI-MS/MS	306:314	arg1	strategy					322:329	a novel ESI-MS/MS based strategy	298:329	a novel ESI-MS/MS based strategy	298:329	Herein, a novel ESI-MS/MS based strategy was established to discriminate and relatively quantified protonated PMP-disaccharides with different composition and glycosidic bond.					
36592600	6	26	theme	rapid	1158:1162	arg1	differentiation					1164:1178	differentiation	1164:1178	differentiation	1164:1178	In general, this method was easy to operation and effective for rapid differentiation and quantification of isomeric disaccharides in complex matrices.					
36592600	5	27	theme	relative	1027:1034	arg1	content					1036:1042	then the relative content	1018:1042	then the relative content of maltose and isomaltose	1018:1068	Ultimately, this method was applied for the discrimination of yellow rice wine, and then the relative content of maltose and isomaltose were confirmed as well.					
36592600	5	27	theme	relative	1027:1034	arg1	well					1088:1091	well	1088:1091	well	1088:1091	Ultimately, this method was applied for the discrimination of yellow rice wine, and then the relative content of maltose and isomaltose were confirmed as well.					
36592600	0	28	theme	yellow	140:145	arg1	wine					152:155	yellow rice wine	140:155	yellow rice wine	140:155	Rapid identification and relative quantification of disaccharide isomers by three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine.					
36592600	3	29	theme	protonated	481:490	arg1	labeled-disaccharides					496:516	protonated PMP labeled-disaccharides	481:516	protonated PMP labeled-disaccharides	481:516	Interestingly, protonated PMP labeled-disaccharides provided abundant fragment ions arising from cross-ring cleavage and glycosidic bond cleavage, which could afford diagnostic fragment patterns for isomers differentiation in combination of statistical analysis.					
36592600	1	30	theme	carbohydrates	215:227	arg1	identification					229:242	carbohydrates identification	215:242	carbohydrates identification	215:242	Small structural differences bring great difficulties on carbohydrates identification, especially in terms of their quantification.					
36592600	3	31	theme	PMP	492:494	arg1	labeled-disaccharides					496:516	protonated PMP labeled-disaccharides	481:516	protonated PMP labeled-disaccharides	481:516	Interestingly, protonated PMP labeled-disaccharides provided abundant fragment ions arising from cross-ring cleavage and glycosidic bond cleavage, which could afford diagnostic fragment patterns for isomers differentiation in combination of statistical analysis.					
36592600	4	32	theme	quantified	894:903	arg1	isomers					905:911	relatively quantified isomers	883:911	relatively quantified isomers	883:911	It was worth to note that the relative intensity ratios (RIR) of three ion pairs could completely discriminate 16 disaccharides, and subsequently used to relatively quantified isomers in a binary mixture.					
36592600	2	33	theme	glycosidic	449:458	arg1	bond					460:463	glycosidic bond	449:463	glycosidic bond	449:463	Herein, a novel ESI-MS/MS based strategy was established to discriminate and relatively quantified protonated PMP-disaccharides with different composition and glycosidic bond.					
36592600	0	34	theme	isomers	65:71	arg1	identification					6:19	Rapid identification	0:19	Rapid identification	0:19	Rapid identification and relative quantification of disaccharide isomers by three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine.					
36592600	0	34	theme	isomers	65:71	arg1	quantification					34:47	relative quantification	25:47	relative quantification	25:47	Rapid identification and relative quantification of disaccharide isomers by three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine.					
36592600	3	35	theme	isomers	665:671	arg1	differentiation					673:687	isomers differentiation	665:687	isomers differentiation	665:687	Interestingly, protonated PMP labeled-disaccharides provided abundant fragment ions arising from cross-ring cleavage and glycosidic bond cleavage, which could afford diagnostic fragment patterns for isomers differentiation in combination of statistical analysis.					
36592600	6	36	from	matrices	1236:1243	arg1	quantification					1184:1197	quantification	1184:1197	quantification	1184:1197	In general, this method was easy to operation and effective for rapid differentiation and quantification of isomeric disaccharides in complex matrices.					
36592600	6	36	from	matrices	1236:1243	arg1	differentiation					1164:1178	differentiation	1164:1178	differentiation	1164:1178	In general, this method was easy to operation and effective for rapid differentiation and quantification of isomeric disaccharides in complex matrices.					
36592600	3	37	theme	cross-ring	563:572	arg1	cleavage					574:581	cross-ring cleavage	563:581	cross-ring cleavage	563:581	Interestingly, protonated PMP labeled-disaccharides provided abundant fragment ions arising from cross-ring cleavage and glycosidic bond cleavage, which could afford diagnostic fragment patterns for isomers differentiation in combination of statistical analysis.					
36592600	0	38	theme	disaccharide	52:63	arg1	isomers					65:71	disaccharide isomers	52:71	disaccharide isomers	52:71	Rapid identification and relative quantification of disaccharide isomers by three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine.					
36592600	0	39	theme	rice	147:150	arg1	wine					152:155	yellow rice wine	140:155	yellow rice wine	140:155	Rapid identification and relative quantification of disaccharide isomers by three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine.					
36592600	6	40	theme	disaccharides	1211:1223	arg1	quantification					1184:1197	quantification	1184:1197	quantification	1184:1197	In general, this method was easy to operation and effective for rapid differentiation and quantification of isomeric disaccharides in complex matrices.					
36592600	6	40	theme	disaccharides	1211:1223	arg1	differentiation					1164:1178	differentiation	1164:1178	differentiation	1164:1178	In general, this method was easy to operation and effective for rapid differentiation and quantification of isomeric disaccharides in complex matrices.					
36592600	3	41	theme	glycosidic	587:596	arg1	cleavage					603:610	glycosidic bond cleavage	587:610	glycosidic bond cleavage	587:610	Interestingly, protonated PMP labeled-disaccharides provided abundant fragment ions arising from cross-ring cleavage and glycosidic bond cleavage, which could afford diagnostic fragment patterns for isomers differentiation in combination of statistical analysis.					
36592600	5	42	theme	yellow	996:1001	arg1	wine					1008:1011	yellow rice wine	996:1011	yellow rice wine	996:1011	Ultimately, this method was applied for the discrimination of yellow rice wine, and then the relative content of maltose and isomaltose were confirmed as well.					
36592600	0	43	from	ESI-MS/MS	107:115	arg1	wine					152:155	yellow rice wine	140:155	yellow rice wine	140:155	Rapid identification and relative quantification of disaccharide isomers by three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine.					
36592600	3	44	theme	analysis	719:726	arg1	combination					692:702	combination	692:702	combination of statistical analysis	692:726	Interestingly, protonated PMP labeled-disaccharides provided abundant fragment ions arising from cross-ring cleavage and glycosidic bond cleavage, which could afford diagnostic fragment patterns for isomers differentiation in combination of statistical analysis.					
36592600	6	45	from	quantification	1184:1197	arg1	matrices					1236:1243	complex matrices	1228:1243	complex matrices	1228:1243	In general, this method was easy to operation and effective for rapid differentiation and quantification of isomeric disaccharides in complex matrices.					
36592600	4	46	theme	ion	800:802	arg1	pairs					804:808	three ion pairs	794:808	three ion pairs	794:808	It was worth to note that the relative intensity ratios (RIR) of three ion pairs could completely discriminate 16 disaccharides, and subsequently used to relatively quantified isomers in a binary mixture.					
36592600	3	47	theme	diagnostic	632:641	arg1	patterns					652:659	diagnostic fragment patterns	632:659	diagnostic fragment patterns for isomers differentiation	632:687	Interestingly, protonated PMP labeled-disaccharides provided abundant fragment ions arising from cross-ring cleavage and glycosidic bond cleavage, which could afford diagnostic fragment patterns for isomers differentiation in combination of statistical analysis.					
36592600	2	48	with	PMP-disaccharides	400:416	arg1	composition					433:443	different composition	423:443	different composition	423:443	Herein, a novel ESI-MS/MS based strategy was established to discriminate and relatively quantified protonated PMP-disaccharides with different composition and glycosidic bond.					
36592600	2	48	with	PMP-disaccharides	400:416	arg1	bond					460:463	glycosidic bond	449:463	glycosidic bond	449:463	Herein, a novel ESI-MS/MS based strategy was established to discriminate and relatively quantified protonated PMP-disaccharides with different composition and glycosidic bond.					
36592600	5	49	theme	isomaltose	1059:1068	arg1	content					1036:1042	then the relative content	1018:1042	then the relative content of maltose and isomaltose	1018:1068	Ultimately, this method was applied for the discrimination of yellow rice wine, and then the relative content of maltose and isomaltose were confirmed as well.					
36592600	5	49	theme	isomaltose	1059:1068	arg1	well					1088:1091	well	1088:1091	well	1088:1091	Ultimately, this method was applied for the discrimination of yellow rice wine, and then the relative content of maltose and isomaltose were confirmed as well.					
36592600	4	50	theme	intensity	768:776	arg1	RIR					786:788	RIR	786:788	RIR	786:788	It was worth to note that the relative intensity ratios (RIR) of three ion pairs could completely discriminate 16 disaccharides, and subsequently used to relatively quantified isomers in a binary mixture.					
36592600	4	50	theme	intensity	768:776	arg1	ratios					778:783	the relative intensity ratios	755:783	the relative intensity ratios (RIR) of three ion pairs	755:808	It was worth to note that the relative intensity ratios (RIR) of three ion pairs could completely discriminate 16 disaccharides, and subsequently used to relatively quantified isomers in a binary mixture.					
36592600	5	51	theme	rice	1003:1006	arg1	wine					1008:1011	yellow rice wine	996:1011	yellow rice wine	996:1011	Ultimately, this method was applied for the discrimination of yellow rice wine, and then the relative content of maltose and isomaltose were confirmed as well.					
36592600	3	52	theme	abundant	527:534	arg1	ions					545:548	abundant fragment ions	527:548	abundant fragment ions	527:548	Interestingly, protonated PMP labeled-disaccharides provided abundant fragment ions arising from cross-ring cleavage and glycosidic bond cleavage, which could afford diagnostic fragment patterns for isomers differentiation in combination of statistical analysis.					
36592600	3	53	theme	bond	598:601	arg1	cleavage					603:610	glycosidic bond cleavage	587:610	glycosidic bond cleavage	587:610	Interestingly, protonated PMP labeled-disaccharides provided abundant fragment ions arising from cross-ring cleavage and glycosidic bond cleavage, which could afford diagnostic fragment patterns for isomers differentiation in combination of statistical analysis.					
36592600	0	54	from	application	125:135	arg1	wine					152:155	yellow rice wine	140:155	yellow rice wine	140:155	Rapid identification and relative quantification of disaccharide isomers by three fragment ion pairs using ESI-MS/MS and its application in yellow rice wine.					
36592600	4	55	used	used	875:878	arg2	RIR					786:788	RIR	786:788	RIR	786:788	It was worth to note that the relative intensity ratios (RIR) of three ion pairs could completely discriminate 16 disaccharides, and subsequently used to relatively quantified isomers in a binary mixture.					
36592600	4	55	used	used	875:878	arg2	ratios					778:783	the relative intensity ratios	755:783	the relative intensity ratios (RIR) of three ion pairs	755:808	It was worth to note that the relative intensity ratios (RIR) of three ion pairs could completely discriminate 16 disaccharides, and subsequently used to relatively quantified isomers in a binary mixture.					
36592600	3	56	theme	statistical	707:717	arg1	analysis					719:726	statistical analysis	707:726	statistical analysis	707:726	Interestingly, protonated PMP labeled-disaccharides provided abundant fragment ions arising from cross-ring cleavage and glycosidic bond cleavage, which could afford diagnostic fragment patterns for isomers differentiation in combination of statistical analysis.					
36592600	2	57	theme	protonated	389:398	arg1	PMP-disaccharides					400:416	and relatively quantified protonated PMP-disaccharides	363:416	PMP-disaccharides	400:416	Herein, a novel ESI-MS/MS based strategy was established to discriminate and relatively quantified protonated PMP-disaccharides with different composition and glycosidic bond.					
36934816	1	0	theme	uranium‑vanadium	356:371	arg1	adsorption					373:382	competitive uranium‑vanadium adsorption	344:382	competitive uranium‑vanadium adsorption	344:382	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	5	1	from	times	850:854	arg1	seawater					758:765	simulated and real seawater	739:765	simulated and real seawater	739:765	In simulated and real seawater, the Kd and adsorption capacity of PACNC for uranium were approximately 8 and 6.62 times those for vanadium, respectively.					
36934816	3	2	theme	PACNC	544:548	arg1	2.83E-2 g mg-1 min-1					566:585	2.83E-2 g mg-1 min-1	566:585	2.83E-2 g mg-1 min-1	566:585	The maximum adsorption rate constant of PACNC for uranium was 2.83E-2 g mg-1 min-1, which is 2.38 times that of ACNC (1.19E-2 g mg-1 min-1).					
36934816	3	2	theme	PACNC	544:548	arg1	rate					527:530	The maximum adsorption rate constant	504:539	The maximum adsorption rate constant of PACNC for uranium	504:560	The maximum adsorption rate constant of PACNC for uranium was 2.83E-2 g mg-1 min-1, which is 2.38 times that of ACNC (1.19E-2 g mg-1 min-1).					
36934816	1	3	dep	rate	335:338	arg1	the					320:322	the	320:322	the	320:322	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	1	4	theme	quaternary	268:277	arg1	ammonium					279:286	quaternary ammonium	268:286	quaternary ammonium	268:286	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	1	5	with	adsorbent	189:197	arg1	groups					220:225	multifunctional groups	204:225	multifunctional groups	204:225	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	1	5	with	adsorbent	189:197	arg1	ammonium					279:286	quaternary ammonium	268:286	quaternary ammonium	268:286	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	1	5	with	adsorbent	189:197	arg1	amidoxime					253:261	amidoxime	253:261	amidoxime	253:261	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	1	5	with	adsorbent	189:197	arg1	acid					247:250	phosphoric acid	236:250	phosphoric acid	236:250	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	4	6	theme	adsorption	659:668	arg1	time					682:685	the adsorption equilibrium time	655:685	the adsorption equilibrium time	655:685	Moreover, the adsorption equilibrium time was shortened from 300 (ACNC) to 50 (PACNC) min.					
36934816	1	7	theme	adsorbent	189:197	arg1	composite					179:187	A novel chitosan-based porous composite	149:187	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups,	149:294	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	6	8	theme	adsorption	1015:1024	arg1	rate					1026:1029	uranium adsorption rate	1007:1029	uranium adsorption rate	1007:1029	These results suggest that phosphorylation significantly improved the competitive adsorption of uranium‑vanadium and uranium adsorption rate.					
36934816	0	9	theme	uranium	115:121	arg1	extraction					123:132	rapid uranium extraction	109:132	rapid uranium extraction from seawater	109:146	Phosphorylation improved the competitive U/V adsorption on chitosan-based adsorbent containing amidoxime for rapid uranium extraction from seawater.					
36934816	2	10	from	308 K	486:490	arg1	962.226 mg g-1					468:481	962.226 mg g-1	468:481	962.226 mg g-1	468:481	The maximum uranium adsorption capacity of PACNC was 962.226 mg g-1 at 308 K and pH = 7.					
36934816	2	10	from	308 K	486:490	arg1	capacity					446:453	The maximum uranium adsorption capacity	415:453	The maximum uranium adsorption capacity of PACNC	415:462	The maximum uranium adsorption capacity of PACNC was 962.226 mg g-1 at 308 K and pH = 7.					
36934816	6	11	theme	uranium	1007:1013	arg1	rate					1026:1029	uranium adsorption rate	1007:1029	uranium adsorption rate	1007:1029	These results suggest that phosphorylation significantly improved the competitive adsorption of uranium‑vanadium and uranium adsorption rate.					
36934816	0	12	theme	rapid	109:113	arg1	extraction					123:132	rapid uranium extraction	109:132	rapid uranium extraction from seawater	109:146	Phosphorylation improved the competitive U/V adsorption on chitosan-based adsorbent containing amidoxime for rapid uranium extraction from seawater.					
36934816	1	13	theme	amidoxime	387:395	arg1	adsorbents					403:412	amidoxime group adsorbents	387:412	amidoxime group adsorbents	387:412	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	9	14	theme	competitive	1370:1380	arg1	adsorption					1382:1391	the competitive adsorption	1366:1391	the competitive adsorption of uranium-vanadium of amidoxime	1366:1424	The binding structure of phosphate and amidoxime is the most stable, and its synergistic effect effectively improves the competitive adsorption of uranium-vanadium of amidoxime.					
36934816	9	15	theme	amidoxime	1416:1424	arg1	uranium-vanadium					1396:1411	uranium-vanadium	1396:1411	uranium-vanadium of amidoxime	1396:1424	The binding structure of phosphate and amidoxime is the most stable, and its synergistic effect effectively improves the competitive adsorption of uranium-vanadium of amidoxime.					
36934816	5	16	theme	simulated	739:747	arg1	seawater					758:765	simulated and real seawater	739:765	simulated and real seawater	739:765	In simulated and real seawater, the Kd and adsorption capacity of PACNC for uranium were approximately 8 and 6.62 times those for vanadium, respectively.					
36934816	1	17	theme	group	397:401	arg1	adsorbents					403:412	amidoxime group adsorbents	387:412	amidoxime group adsorbents	387:412	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	3	18	theme	maximum	508:514	arg1	2.83E-2 g mg-1 min-1					566:585	2.83E-2 g mg-1 min-1	566:585	2.83E-2 g mg-1 min-1	566:585	The maximum adsorption rate constant of PACNC for uranium was 2.83E-2 g mg-1 min-1, which is 2.38 times that of ACNC (1.19E-2 g mg-1 min-1).					
36934816	3	18	theme	maximum	508:514	arg1	rate					527:530	The maximum adsorption rate constant	504:539	The maximum adsorption rate constant of PACNC for uranium	504:560	The maximum adsorption rate constant of PACNC for uranium was 2.83E-2 g mg-1 min-1, which is 2.38 times that of ACNC (1.19E-2 g mg-1 min-1).					
36934816	10	19	theme	uranium	1509:1515	arg1	extraction					1517:1526	uranium extraction	1509:1526	uranium extraction from seawater	1509:1540	All the results demonstrated that PACNC has substantial application potential for uranium extraction from seawater.					
36934816	2	20	theme	adsorption	435:444	arg1	962.226 mg g-1					468:481	962.226 mg g-1	468:481	962.226 mg g-1	468:481	The maximum uranium adsorption capacity of PACNC was 962.226 mg g-1 at 308 K and pH = 7.					
36934816	2	20	theme	adsorption	435:444	arg1	capacity					446:453	The maximum uranium adsorption capacity	415:453	The maximum uranium adsorption capacity of PACNC	415:462	The maximum uranium adsorption capacity of PACNC was 962.226 mg g-1 at 308 K and pH = 7.					
36934816	8	21	theme	co-complex	1206:1215	arg1	structure					1217:1225	the possible co-complex structure	1193:1225	the possible co-complex structure of PACNC and uranium	1193:1246	DFT calculations were used to analyze and calculate the possible co-complex structure of PACNC and uranium.					
36934816	0	22	from	adsorption	45:54	arg1	amidoxime					95:103	chitosan-based adsorbent containing amidoxime	59:103	chitosan-based adsorbent containing amidoxime	59:103	Phosphorylation improved the competitive U/V adsorption on chitosan-based adsorbent containing amidoxime for rapid uranium extraction from seawater.					
36934816	6	23	theme	rate	1026:1029	arg1	adsorption					972:981	the competitive adsorption	956:981	the competitive adsorption of uranium‑vanadium and uranium adsorption rate	956:1029	These results suggest that phosphorylation significantly improved the competitive adsorption of uranium‑vanadium and uranium adsorption rate.					
36934816	10	24	theme	application	1483:1493	arg1	potential					1495:1503	substantial application potential	1471:1503	substantial application potential	1471:1503	All the results demonstrated that PACNC has substantial application potential for uranium extraction from seawater.					
36934816	1	25	theme	multifunctional	204:218	arg1	groups					220:225	multifunctional groups	204:225	multifunctional groups	204:225	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	1	25	theme	multifunctional	204:218	arg1	ammonium					279:286	quaternary ammonium	268:286	quaternary ammonium	268:286	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	1	25	theme	multifunctional	204:218	arg1	amidoxime					253:261	amidoxime	253:261	amidoxime	253:261	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	1	25	theme	multifunctional	204:218	arg1	acid					247:250	phosphoric acid	236:250	phosphoric acid	236:250	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	8	26	theme	PACNC	1230:1234	arg1	structure					1217:1225	the possible co-complex structure	1193:1225	the possible co-complex structure of PACNC and uranium	1193:1246	DFT calculations were used to analyze and calculate the possible co-complex structure of PACNC and uranium.					
36934816	9	27	theme	uranium-vanadium	1396:1411	arg1	adsorption					1382:1391	the competitive adsorption	1366:1391	the competitive adsorption of uranium-vanadium of amidoxime	1366:1424	The binding structure of phosphate and amidoxime is the most stable, and its synergistic effect effectively improves the competitive adsorption of uranium-vanadium of amidoxime.					
36934816	5	28	dep	Kd	772:773	arg1	the					768:770	the	768:770	the	768:770	In simulated and real seawater, the Kd and adsorption capacity of PACNC for uranium were approximately 8 and 6.62 times those for vanadium, respectively.					
36934816	5	29	theme	real	753:756	arg1	seawater					758:765	simulated and real seawater	739:765	simulated and real seawater	739:765	In simulated and real seawater, the Kd and adsorption capacity of PACNC for uranium were approximately 8 and 6.62 times those for vanadium, respectively.					
36934816	2	30	from	pH = 7	496:501	arg1	962.226 mg g-1					468:481	962.226 mg g-1	468:481	962.226 mg g-1	468:481	The maximum uranium adsorption capacity of PACNC was 962.226 mg g-1 at 308 K and pH = 7.					
36934816	2	30	from	pH = 7	496:501	arg1	capacity					446:453	The maximum uranium adsorption capacity	415:453	The maximum uranium adsorption capacity of PACNC	415:462	The maximum uranium adsorption capacity of PACNC was 962.226 mg g-1 at 308 K and pH = 7.					
36934816	2	31	theme	maximum	419:425	arg1	962.226 mg g-1					468:481	962.226 mg g-1	468:481	962.226 mg g-1	468:481	The maximum uranium adsorption capacity of PACNC was 962.226 mg g-1 at 308 K and pH = 7.					
36934816	2	31	theme	maximum	419:425	arg1	capacity					446:453	The maximum uranium adsorption capacity	415:453	The maximum uranium adsorption capacity of PACNC	415:462	The maximum uranium adsorption capacity of PACNC was 962.226 mg g-1 at 308 K and pH = 7.					
36934816	5	32	theme	PACNC	802:806	arg1	Kd					772:773	Kd	772:773	Kd	772:773	In simulated and real seawater, the Kd and adsorption capacity of PACNC for uranium were approximately 8 and 6.62 times those for vanadium, respectively.					
36934816	5	32	theme	PACNC	802:806	arg1	capacity					790:797	adsorption capacity	779:797	adsorption capacity	779:797	In simulated and real seawater, the Kd and adsorption capacity of PACNC for uranium were approximately 8 and 6.62 times those for vanadium, respectively.					
36934816	6	33	theme	uranium‑vanadium	986:1001	arg1	adsorption					972:981	the competitive adsorption	956:981	the competitive adsorption of uranium‑vanadium and uranium adsorption rate	956:1029	These results suggest that phosphorylation significantly improved the competitive adsorption of uranium‑vanadium and uranium adsorption rate.					
36934816	10	34	contain	has	1467:1469	arg2	potential					1495:1503	substantial application potential	1471:1503	substantial application potential	1471:1503	All the results demonstrated that PACNC has substantial application potential for uranium extraction from seawater.					
36934816	10	34	contain	has	1467:1469	arg1	PACNC					1461:1465	PACNC	1461:1465	PACNC	1461:1465	All the results demonstrated that PACNC has substantial application potential for uranium extraction from seawater.					
36934816	9	35	theme	binding	1253:1259	arg1	stable					1310:1315	stable	1310:1315	stable	1310:1315	The binding structure of phosphate and amidoxime is the most stable, and its synergistic effect effectively improves the competitive adsorption of uranium-vanadium of amidoxime.					
36934816	9	35	theme	binding	1253:1259	arg1	structure					1261:1269	The binding structure	1249:1269	The binding structure of phosphate and amidoxime	1249:1296	The binding structure of phosphate and amidoxime is the most stable, and its synergistic effect effectively improves the competitive adsorption of uranium-vanadium of amidoxime.					
36934816	4	36	theme	300	706:708	arg1	min					731:733	300 (ACNC) to 50 (PACNC) min	706:733	300 (ACNC) to 50 (PACNC) min	706:733	Moreover, the adsorption equilibrium time was shortened from 300 (ACNC) to 50 (PACNC) min.					
36934816	0	37	theme	competitive	29:39	arg1	adsorption					45:54	the competitive U/V adsorption	25:54	the competitive U/V adsorption on chitosan-based adsorbent containing amidoxime for rapid uranium extraction from seawater	25:146	Phosphorylation improved the competitive U/V adsorption on chitosan-based adsorbent containing amidoxime for rapid uranium extraction from seawater.					
36934816	8	38	used	used	1163:1166	arg2	calculations					1145:1156	DFT calculations	1141:1156	DFT calculations	1141:1156	DFT calculations were used to analyze and calculate the possible co-complex structure of PACNC and uranium.					
36934816	7	39	theme	adsorption	1102:1111	arg1	capacity					1113:1120	stable adsorption capacity	1095:1120	stable adsorption capacity	1095:1120	PACNC also exhibited good recycling performance and maintained stable adsorption capacity after five cycles.					
36934816	5	40	theme	adsorption	779:788	arg1	capacity					790:797	adsorption capacity	779:797	adsorption capacity	779:797	In simulated and real seawater, the Kd and adsorption capacity of PACNC for uranium were approximately 8 and 6.62 times those for vanadium, respectively.					
36934816	4	41	theme	equilibrium	670:680	arg1	time					682:685	the adsorption equilibrium time	655:685	the adsorption equilibrium time	655:685	Moreover, the adsorption equilibrium time was shortened from 300 (ACNC) to 50 (PACNC) min.					
36934816	2	42	theme	PACNC	458:462	arg1	962.226 mg g-1					468:481	962.226 mg g-1	468:481	962.226 mg g-1	468:481	The maximum uranium adsorption capacity of PACNC was 962.226 mg g-1 at 308 K and pH = 7.					
36934816	2	42	theme	PACNC	458:462	arg1	capacity					446:453	The maximum uranium adsorption capacity	415:453	The maximum uranium adsorption capacity of PACNC	415:462	The maximum uranium adsorption capacity of PACNC was 962.226 mg g-1 at 308 K and pH = 7.					
36934816	6	43	theme	competitive	960:970	arg1	adsorption					972:981	the competitive adsorption	956:981	the competitive adsorption of uranium‑vanadium and uranium adsorption rate	956:1029	These results suggest that phosphorylation significantly improved the competitive adsorption of uranium‑vanadium and uranium adsorption rate.					
36934816	9	44	theme	phosphate	1274:1282	arg1	stable					1310:1315	stable	1310:1315	stable	1310:1315	The binding structure of phosphate and amidoxime is the most stable, and its synergistic effect effectively improves the competitive adsorption of uranium-vanadium of amidoxime.					
36934816	9	44	theme	phosphate	1274:1282	arg1	structure					1261:1269	The binding structure	1249:1269	The binding structure of phosphate and amidoxime	1249:1296	The binding structure of phosphate and amidoxime is the most stable, and its synergistic effect effectively improves the competitive adsorption of uranium-vanadium of amidoxime.					
36934816	1	45	theme	phosphoric	236:245	arg1	acid					247:250	phosphoric acid	236:250	phosphoric acid	236:250	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	0	46	theme	U/V	41:43	arg1	adsorption					45:54	the competitive U/V adsorption	25:54	the competitive U/V adsorption on chitosan-based adsorbent containing amidoxime for rapid uranium extraction from seawater	25:146	Phosphorylation improved the competitive U/V adsorption on chitosan-based adsorbent containing amidoxime for rapid uranium extraction from seawater.					
36934816	10	47	from	seawater	1533:1540	arg1	extraction					1517:1526	uranium extraction	1509:1526	uranium extraction from seawater	1509:1540	All the results demonstrated that PACNC has substantial application potential for uranium extraction from seawater.					
36934816	7	48	theme	stable	1095:1100	arg1	capacity					1113:1120	stable adsorption capacity	1095:1120	stable adsorption capacity	1095:1120	PACNC also exhibited good recycling performance and maintained stable adsorption capacity after five cycles.					
36934816	8	49	theme	uranium	1240:1246	arg1	structure					1217:1225	the possible co-complex structure	1193:1225	the possible co-complex structure of PACNC and uranium	1193:1246	DFT calculations were used to analyze and calculate the possible co-complex structure of PACNC and uranium.					
36934816	3	50	dep	times	602:606	arg1	that					608:611	that	608:611	that	608:611	The maximum adsorption rate constant of PACNC for uranium was 2.83E-2 g mg-1 min-1, which is 2.38 times that of ACNC (1.19E-2 g mg-1 min-1).					
36934816	0	51	theme	chitosan-based	59:72	arg1	amidoxime					95:103	chitosan-based adsorbent containing amidoxime	59:103	chitosan-based adsorbent containing amidoxime	59:103	Phosphorylation improved the competitive U/V adsorption on chitosan-based adsorbent containing amidoxime for rapid uranium extraction from seawater.					
36934816	1	52	theme	adsorption	324:333	arg1	rate					335:338	adsorption rate	324:338	adsorption rate	324:338	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	9	53	theme	amidoxime	1288:1296	arg1	stable					1310:1315	stable	1310:1315	stable	1310:1315	The binding structure of phosphate and amidoxime is the most stable, and its synergistic effect effectively improves the competitive adsorption of uranium-vanadium of amidoxime.					
36934816	9	53	theme	amidoxime	1288:1296	arg1	structure					1261:1269	The binding structure	1249:1269	The binding structure of phosphate and amidoxime	1249:1296	The binding structure of phosphate and amidoxime is the most stable, and its synergistic effect effectively improves the competitive adsorption of uranium-vanadium of amidoxime.					
36934816	3	54	theme	ACNC	616:619	arg1	times					602:606	2.38 times	597:606	2.38 times that of ACNC (1.19E-2 g mg-1 min-1)	597:642	The maximum adsorption rate constant of PACNC for uranium was 2.83E-2 g mg-1 min-1, which is 2.38 times that of ACNC (1.19E-2 g mg-1 min-1).					
36934816	1	55	theme	adsorbents	403:412	arg1	adsorption					373:382	competitive uranium‑vanadium adsorption	344:382	competitive uranium‑vanadium adsorption	344:382	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	1	55	theme	adsorbents	403:412	arg1	rate					335:338	adsorption rate	324:338	adsorption rate	324:338	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	3	56	theme	constant	532:539	arg1	2.83E-2 g mg-1 min-1					566:585	2.83E-2 g mg-1 min-1	566:585	2.83E-2 g mg-1 min-1	566:585	The maximum adsorption rate constant of PACNC for uranium was 2.83E-2 g mg-1 min-1, which is 2.38 times that of ACNC (1.19E-2 g mg-1 min-1).					
36934816	3	56	theme	constant	532:539	arg1	rate					527:530	The maximum adsorption rate constant	504:539	The maximum adsorption rate constant of PACNC for uranium	504:560	The maximum adsorption rate constant of PACNC for uranium was 2.83E-2 g mg-1 min-1, which is 2.38 times that of ACNC (1.19E-2 g mg-1 min-1).					
36934816	9	57	theme	synergistic	1326:1336	arg1	effect					1338:1343	its synergistic effect	1322:1343	its synergistic effect	1322:1343	The binding structure of phosphate and amidoxime is the most stable, and its synergistic effect effectively improves the competitive adsorption of uranium-vanadium of amidoxime.					
36934816	8	58	theme	DFT	1141:1143	arg1	calculations					1145:1156	DFT calculations	1141:1156	DFT calculations	1141:1156	DFT calculations were used to analyze and calculate the possible co-complex structure of PACNC and uranium.					
36934816	5	59	dep	times	850:854	arg1	those					856:860	those	856:860	those	856:860	In simulated and real seawater, the Kd and adsorption capacity of PACNC for uranium were approximately 8 and 6.62 times those for vanadium, respectively.					
36934816	7	60	theme	good	1053:1056	arg1	performance					1068:1078	good recycling performance	1053:1078	good recycling performance	1053:1078	PACNC also exhibited good recycling performance and maintained stable adsorption capacity after five cycles.					
36934816	3	61	theme	adsorption	516:525	arg1	2.83E-2 g mg-1 min-1					566:585	2.83E-2 g mg-1 min-1	566:585	2.83E-2 g mg-1 min-1	566:585	The maximum adsorption rate constant of PACNC for uranium was 2.83E-2 g mg-1 min-1, which is 2.38 times that of ACNC (1.19E-2 g mg-1 min-1).					
36934816	3	61	theme	adsorption	516:525	arg1	rate					527:530	The maximum adsorption rate constant	504:539	The maximum adsorption rate constant of PACNC for uranium	504:560	The maximum adsorption rate constant of PACNC for uranium was 2.83E-2 g mg-1 min-1, which is 2.38 times that of ACNC (1.19E-2 g mg-1 min-1).					
36934816	8	62	theme	possible	1197:1204	arg1	structure					1217:1225	the possible co-complex structure	1193:1225	the possible co-complex structure of PACNC and uranium	1193:1246	DFT calculations were used to analyze and calculate the possible co-complex structure of PACNC and uranium.					
36934816	7	63	theme	recycling	1058:1066	arg1	performance					1068:1078	good recycling performance	1053:1078	good recycling performance	1053:1078	PACNC also exhibited good recycling performance and maintained stable adsorption capacity after five cycles.					
36934816	1	64	dep	acid	247:250	arg1	groups					288:293	groups	288:293	groups	288:293	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	1	65	theme	novel	151:155	arg1	composite					179:187	A novel chitosan-based porous composite	149:187	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups,	149:294	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	0	66	theme	containing	84:93	arg1	amidoxime					95:103	chitosan-based adsorbent containing amidoxime	59:103	chitosan-based adsorbent containing amidoxime	59:103	Phosphorylation improved the competitive U/V adsorption on chitosan-based adsorbent containing amidoxime for rapid uranium extraction from seawater.					
36934816	1	67	theme	chitosan-based	157:170	arg1	composite					179:187	A novel chitosan-based porous composite	149:187	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups,	149:294	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	2	68	theme	uranium	427:433	arg1	962.226 mg g-1					468:481	962.226 mg g-1	468:481	962.226 mg g-1	468:481	The maximum uranium adsorption capacity of PACNC was 962.226 mg g-1 at 308 K and pH = 7.					
36934816	2	68	theme	uranium	427:433	arg1	capacity					446:453	The maximum uranium adsorption capacity	415:453	The maximum uranium adsorption capacity of PACNC	415:462	The maximum uranium adsorption capacity of PACNC was 962.226 mg g-1 at 308 K and pH = 7.					
36934816	0	69	theme	adsorbent	74:82	arg1	amidoxime					95:103	chitosan-based adsorbent containing amidoxime	59:103	chitosan-based adsorbent containing amidoxime	59:103	Phosphorylation improved the competitive U/V adsorption on chitosan-based adsorbent containing amidoxime for rapid uranium extraction from seawater.					
36934816	1	70	theme	competitive	344:354	arg1	adsorption					373:382	competitive uranium‑vanadium adsorption	344:382	competitive uranium‑vanadium adsorption	344:382	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	10	71	theme	substantial	1471:1481	arg1	potential					1495:1503	substantial application potential	1471:1503	substantial application potential	1471:1503	All the results demonstrated that PACNC has substantial application potential for uranium extraction from seawater.					
36934816	4	72	dep	300	706:708	arg1	to					717:718	to	717:718	to	717:718	Moreover, the adsorption equilibrium time was shortened from 300 (ACNC) to 50 (PACNC) min.					
36934816	4	72	dep	300	706:708	arg1	50					720:721	50	720:721	50	720:721	Moreover, the adsorption equilibrium time was shortened from 300 (ACNC) to 50 (PACNC) min.					
36934816	1	73	theme	porous	172:177	arg1	composite					179:187	A novel chitosan-based porous composite	149:187	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups,	149:294	A novel chitosan-based porous composite adsorbent with multifunctional groups, such as phosphoric acid, amidoxime, and quaternary ammonium groups, was prepared to improve the adsorption rate and competitive uranium‑vanadium adsorption of amidoxime group adsorbents.					
36934816	0	74	from	seawater	139:146	arg1	extraction					123:132	rapid uranium extraction	109:132	rapid uranium extraction from seawater	109:146	Phosphorylation improved the competitive U/V adsorption on chitosan-based adsorbent containing amidoxime for rapid uranium extraction from seawater.					
36139004	1	0	theme	composite	260:268	arg1	materials					270:278	composite materials	260:278	composite materials	260:278	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	1	1	from	properties	175:184	arg1	fields					220:225	many scientific and industrial fields	189:225	many scientific and industrial fields	189:225	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	6	2	theme	properties	1100:1109	arg1	maintenance					1069:1079	the maintenance	1065:1079	the maintenance of the luminescent properties	1065:1109	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	6	3	theme	wet	991:993	arg1	matrices					995:1002	dry and wet matrices	983:1002	dry and wet matrices	983:1002	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	6	3	theme	wet	991:993	arg1	gels					1023:1026	gels	1023:1026	gels	1023:1026	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	6	3	theme	wet	991:993	arg1	films					1013:1017	films	1013:1017	films	1013:1017	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	1	4	from	material	149:156	arg1	particular					231:240	particular	231:240	particular	231:240	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	3	5	theme	first	539:543	arg1	incorporation					545:557	the first incorporation	535:557	the first incorporation of an organometallic luminescent probe in this biomaterial	535:616	In this paper, we present the covalent linkage of a luminescent Ir-complex onto the surface of MCC, representing the first incorporation of an organometallic luminescent probe in this biomaterial.					
36139004	3	6	from	incorporation	545:557	arg1	biomaterial					606:616	this biomaterial	601:616	this biomaterial	601:616	In this paper, we present the covalent linkage of a luminescent Ir-complex onto the surface of MCC, representing the first incorporation of an organometallic luminescent probe in this biomaterial.					
36139004	4	7	theme	esterification	752:765	arg1	reaction					767:774	the esterification reaction	748:774	the esterification reaction of -OH cellulose surface groups	748:806	This goal has been achieved with an easy and sustainable procedure, which employs a Bronsted-acid ionic liquid as a catalyst for the esterification reaction of -OH cellulose surface groups.					
36139004	3	8	theme	Ir-complex	486:495	arg1	linkage					461:467	the covalent linkage	448:467	the covalent linkage of a luminescent Ir-complex onto the surface of MCC	448:519	In this paper, we present the covalent linkage of a luminescent Ir-complex onto the surface of MCC, representing the first incorporation of an organometallic luminescent probe in this biomaterial.					
36139004	4	9	theme	-OH	779:781	arg1	groups					801:806	-OH cellulose surface groups	779:806	-OH cellulose surface groups	779:806	This goal has been achieved with an easy and sustainable procedure, which employs a Bronsted-acid ionic liquid as a catalyst for the esterification reaction of -OH cellulose surface groups.					
36139004	1	10	theme	many	189:192	arg1	fields					220:225	many scientific and industrial fields	189:225	many scientific and industrial fields	189:225	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	6	11	theme	dry	983:985	arg1	matrices					995:1002	dry and wet matrices	983:1002	dry and wet matrices	983:1002	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	6	11	theme	dry	983:985	arg1	gels					1023:1026	gels	1023:1026	gels	1023:1026	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	6	11	theme	dry	983:985	arg1	films					1013:1017	films	1013:1017	films	1013:1017	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	1	12	with	material	149:156	arg1	properties					175:184	outstanding properties	163:184	outstanding properties in many scientific and industrial fields	163:225	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	1	13	theme	Microcrystalline	101:116	arg1	material					149:156	an emerging material	137:156	an emerging material with outstanding properties in many scientific and industrial fields	137:225	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	1	13	theme	Microcrystalline	101:116	arg1	MCC					129:131	MCC	129:131	MCC	129:131	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	1	13	theme	Microcrystalline	101:116	arg1	cellulose					118:126	Microcrystalline cellulose	101:126	Microcrystalline cellulose (MCC)	101:132	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	1	14	theme	scientific	194:203	arg1	fields					220:225	many scientific and industrial fields	189:225	many scientific and industrial fields	189:225	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	0	15	theme	Cellulose	23:31	arg1	Microcrystals					33:45	Iridium-Functionalized Cellulose Microcrystals	0:45	Iridium-Functionalized Cellulose Microcrystals as a Novel Luminescent Biomaterial for Biocomposites.	0:99	Iridium-Functionalized Cellulose Microcrystals as a Novel Luminescent Biomaterial for Biocomposites.					
36139004	3	16	attach	present	440:446	arg2	we					437:438	we	437:438	we	437:438	In this paper, we present the covalent linkage of a luminescent Ir-complex onto the surface of MCC, representing the first incorporation of an organometallic luminescent probe in this biomaterial.					
36139004	3	16	attach	present	440:446	arg1	paper					430:434	this paper	425:434	this paper	425:434	In this paper, we present the covalent linkage of a luminescent Ir-complex onto the surface of MCC, representing the first incorporation of an organometallic luminescent probe in this biomaterial.					
36139004	3	17	theme	organometallic	565:578	arg1	probe					592:596	an organometallic luminescent probe	562:596	an organometallic luminescent probe	562:596	In this paper, we present the covalent linkage of a luminescent Ir-complex onto the surface of MCC, representing the first incorporation of an organometallic luminescent probe in this biomaterial.					
36139004	0	18	theme	Iridium-Functionalized	0:21	arg1	Microcrystals					33:45	Iridium-Functionalized Cellulose Microcrystals	0:45	Iridium-Functionalized Cellulose Microcrystals as a Novel Luminescent Biomaterial for Biocomposites.	0:99	Iridium-Functionalized Cellulose Microcrystals as a Novel Luminescent Biomaterial for Biocomposites.					
36139004	4	19	theme	liquid	723:728	arg1	ionic					717:721	a Bronsted-acid ionic	701:721	a Bronsted-acid ionic liquid as a catalyst for the esterification reaction of -OH cellulose surface groups	701:806	This goal has been achieved with an easy and sustainable procedure, which employs a Bronsted-acid ionic liquid as a catalyst for the esterification reaction of -OH cellulose surface groups.					
36139004	5	20	theme	stable	875:880	arg1	emissions					882:890	high and stable emissions	866:890	high and stable emissions	866:890	The obtained luminescent cellulose microcrystals display high and stable emissions with the incorporation of only a small amount of iridium (III).					
36139004	3	21	theme	luminescent	580:590	arg1	probe					592:596	an organometallic luminescent probe	562:596	an organometallic luminescent probe	562:596	In this paper, we present the covalent linkage of a luminescent Ir-complex onto the surface of MCC, representing the first incorporation of an organometallic luminescent probe in this biomaterial.					
36139004	1	22	theme	industrial	209:218	arg1	fields					220:225	many scientific and industrial fields	189:225	many scientific and industrial fields	189:225	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	5	23	theme	luminescent	822:832	arg1	microcrystals					844:856	The obtained luminescent cellulose microcrystals	809:856	The obtained luminescent cellulose microcrystals	809:856	The obtained luminescent cellulose microcrystals display high and stable emissions with the incorporation of only a small amount of iridium (III).					
36139004	5	24	theme	obtained	813:820	arg1	microcrystals					844:856	The obtained luminescent cellulose microcrystals	809:856	The obtained luminescent cellulose microcrystals	809:856	The obtained luminescent cellulose microcrystals display high and stable emissions with the incorporation of only a small amount of iridium (III).					
36139004	5	25	theme	iridium	941:947	arg1	amount					931:936	only a small amount	918:936	only a small amount of iridium (III)	918:953	The obtained luminescent cellulose microcrystals display high and stable emissions with the incorporation of only a small amount of iridium (III).					
36139004	5	25	theme	iridium	941:947	arg1	iridium					941:947	iridium (III)	941:953	iridium (III)	941:953	The obtained luminescent cellulose microcrystals display high and stable emissions with the incorporation of only a small amount of iridium (III).					
36139004	6	26	theme	possible	1119:1126	arg1	manufacturers					1134:1146	possible final manufacturers	1119:1146	possible final manufacturers	1119:1146	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	5	27	theme	cellulose	834:842	arg1	microcrystals					844:856	The obtained luminescent cellulose microcrystals	809:856	The obtained luminescent cellulose microcrystals	809:856	The obtained luminescent cellulose microcrystals display high and stable emissions with the incorporation of only a small amount of iridium (III).					
36139004	6	28	from	Incorporation	956:968	arg1	matrices					995:1002	dry and wet matrices	983:1002	dry and wet matrices	983:1002	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	6	28	from	Incorporation	956:968	arg1	gels					1023:1026	gels	1023:1026	gels	1023:1026	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	6	28	from	Incorporation	956:968	arg1	films					1013:1017	films	1013:1017	films	1013:1017	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	6	29	theme	final	1128:1132	arg1	manufacturers					1134:1146	possible final manufacturers	1119:1146	possible final manufacturers	1119:1146	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	0	30	theme	Novel	52:56	arg1	Biomaterial					70:80	a Novel Luminescent Biomaterial	50:80	a Novel Luminescent Biomaterial for Biocomposites	50:98	Iridium-Functionalized Cellulose Microcrystals as a Novel Luminescent Biomaterial for Biocomposites.					
36139004	5	31	theme	amount	931:936	arg1	incorporation					901:913	the incorporation	897:913	the incorporation of only a small amount of iridium (III)	897:953	The obtained luminescent cellulose microcrystals display high and stable emissions with the incorporation of only a small amount of iridium (III).					
36139004	5	32	theme	high	866:869	arg1	emissions					882:890	high and stable emissions	866:890	high and stable emissions	866:890	The obtained luminescent cellulose microcrystals display high and stable emissions with the incorporation of only a small amount of iridium (III).					
36139004	4	33	theme	sustainable	664:674	arg1	procedure					676:684	an easy and sustainable procedure	652:684	an easy and sustainable procedure	652:684	This goal has been achieved with an easy and sustainable procedure, which employs a Bronsted-acid ionic liquid as a catalyst for the esterification reaction of -OH cellulose surface groups.					
36139004	3	34	theme	covalent	452:459	arg1	linkage					461:467	the covalent linkage	448:467	the covalent linkage of a luminescent Ir-complex onto the surface of MCC	448:519	In this paper, we present the covalent linkage of a luminescent Ir-complex onto the surface of MCC, representing the first incorporation of an organometallic luminescent probe in this biomaterial.					
36139004	2	35	theme	applications	408:419	arg1	plethora					396:403	a plethora	394:403	a plethora of applications	394:419	Its surface modification allows for the fine-tuning of its properties and the exploitation of these materials in a plethora of applications.					
36139004	1	36	from	materials	270:278	arg1	additive					248:255	additive	248:255	additive	248:255	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	1	37	from	additive	248:255	arg1	materials					270:278	composite materials	260:278	composite materials	260:278	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	6	38	theme	MCC-Ir	973:978	arg1	Incorporation					956:968	Incorporation	956:968	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels,	956:1027	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	3	39	theme	probe	592:596	arg1	incorporation					545:557	the first incorporation	535:557	the first incorporation of an organometallic luminescent probe in this biomaterial	535:616	In this paper, we present the covalent linkage of a luminescent Ir-complex onto the surface of MCC, representing the first incorporation of an organometallic luminescent probe in this biomaterial.					
36139004	6	40	theme	luminescent	1088:1098	arg1	properties					1100:1109	the luminescent properties	1084:1109	the luminescent properties	1084:1109	Incorporation of MCC-Ir in dry and wet matrices, such as films and gels, has been also demonstrated, showing the maintenance of the luminescent properties even in possible final manufacturers.					
36139004	5	41	theme	small	925:929	arg1	amount					931:936	only a small amount	918:936	only a small amount of iridium (III)	918:953	The obtained luminescent cellulose microcrystals display high and stable emissions with the incorporation of only a small amount of iridium (III).					
36139004	5	41	theme	small	925:929	arg1	iridium					941:947	iridium (III)	941:953	iridium (III)	941:953	The obtained luminescent cellulose microcrystals display high and stable emissions with the incorporation of only a small amount of iridium (III).					
36139004	0	42	theme	Luminescent	58:68	arg1	Biomaterial					70:80	a Novel Luminescent Biomaterial	50:80	a Novel Luminescent Biomaterial for Biocomposites	50:98	Iridium-Functionalized Cellulose Microcrystals as a Novel Luminescent Biomaterial for Biocomposites.					
36139004	1	43	theme	outstanding	163:173	arg1	properties					175:184	outstanding properties	163:184	outstanding properties in many scientific and industrial fields	163:225	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	4	44	theme	Bronsted-acid	703:715	arg1	ionic					717:721	a Bronsted-acid ionic	701:721	a Bronsted-acid ionic liquid as a catalyst for the esterification reaction of -OH cellulose surface groups	701:806	This goal has been achieved with an easy and sustainable procedure, which employs a Bronsted-acid ionic liquid as a catalyst for the esterification reaction of -OH cellulose surface groups.					
36139004	4	45	theme	surface	793:799	arg1	groups					801:806	-OH cellulose surface groups	779:806	-OH cellulose surface groups	779:806	This goal has been achieved with an easy and sustainable procedure, which employs a Bronsted-acid ionic liquid as a catalyst for the esterification reaction of -OH cellulose surface groups.					
36139004	1	46	theme	emerging	140:147	arg1	material					149:156	an emerging material	137:156	an emerging material with outstanding properties in many scientific and industrial fields	137:225	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	1	46	theme	emerging	140:147	arg1	cellulose					118:126	Microcrystalline cellulose	101:126	Microcrystalline cellulose (MCC)	101:132	Microcrystalline cellulose (MCC) is an emerging material with outstanding properties in many scientific and industrial fields, in particular as an additive in composite materials.					
36139004	2	47	theme	surface	285:291	arg1	modification					293:304	Its surface modification	281:304	Its surface modification	281:304	Its surface modification allows for the fine-tuning of its properties and the exploitation of these materials in a plethora of applications.					
36139004	4	48	theme	cellulose	783:791	arg1	groups					801:806	-OH cellulose surface groups	779:806	-OH cellulose surface groups	779:806	This goal has been achieved with an easy and sustainable procedure, which employs a Bronsted-acid ionic liquid as a catalyst for the esterification reaction of -OH cellulose surface groups.					
36139004	2	49	theme	properties	340:349	arg1	fine-tuning					321:331	the fine-tuning	317:331	the fine-tuning of its properties	317:349	Its surface modification allows for the fine-tuning of its properties and the exploitation of these materials in a plethora of applications.					
36139004	2	49	theme	properties	340:349	arg1	exploitation					359:370	the exploitation	355:370	the exploitation of these materials	355:389	Its surface modification allows for the fine-tuning of its properties and the exploitation of these materials in a plethora of applications.					
36139004	3	50	theme	luminescent	474:484	arg1	Ir-complex					486:495	a luminescent Ir-complex	472:495	a luminescent Ir-complex	472:495	In this paper, we present the covalent linkage of a luminescent Ir-complex onto the surface of MCC, representing the first incorporation of an organometallic luminescent probe in this biomaterial.					
36139004	3	51	theme	MCC	517:519	arg1	surface					506:512	the surface	502:512	the surface of MCC	502:519	In this paper, we present the covalent linkage of a luminescent Ir-complex onto the surface of MCC, representing the first incorporation of an organometallic luminescent probe in this biomaterial.					
36139004	4	52	theme	easy	655:658	arg1	procedure					676:684	an easy and sustainable procedure	652:684	an easy and sustainable procedure	652:684	This goal has been achieved with an easy and sustainable procedure, which employs a Bronsted-acid ionic liquid as a catalyst for the esterification reaction of -OH cellulose surface groups.					
36139004	4	53	theme	groups	801:806	arg1	reaction					767:774	the esterification reaction	748:774	the esterification reaction of -OH cellulose surface groups	748:806	This goal has been achieved with an easy and sustainable procedure, which employs a Bronsted-acid ionic liquid as a catalyst for the esterification reaction of -OH cellulose surface groups.					
36139004	2	54	theme	materials	381:389	arg1	fine-tuning					321:331	the fine-tuning	317:331	the fine-tuning of its properties	317:349	Its surface modification allows for the fine-tuning of its properties and the exploitation of these materials in a plethora of applications.					
36139004	2	54	theme	materials	381:389	arg1	exploitation					359:370	the exploitation	355:370	the exploitation of these materials	355:389	Its surface modification allows for the fine-tuning of its properties and the exploitation of these materials in a plethora of applications.					
37194800	5	0	dep	min	1139:1141	arg1	deochlor					1151:1158	1% deochlor	1148:1158	3% H2O2 10 (min); b) 1% deochlor (7 min)	1127:1166	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	0	dep	min	1139:1141	arg1	b					1145:1145	b	1145:1145	b	1145:1145	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	8	1	theme	mg	1638:1639	arg1	BAP					1645:1647	0.3 mg L-1 BAP	1634:1647	0.3 mg L-1 BAP	1634:1647	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	5	2	from	"	1199:1199	arg1	dilution					1208:1215	the dilution	1204:1215	the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1204:1309	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	3	theme	0.1	1081:1083	arg1	%					1084:1084	%	1084:1084	%	1084:1084	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	2	4	theme	studies	331:337	arg1	result					317:322	the result	313:322	the result of our studies	313:337	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	5	5	with	dilution	1208:1215	arg1	HgCl2					1267:1271	subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1241:1309	subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1241:1309	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	8	6	theme	0.01	1650:1653	arg1	mg					1655:1656	mg	1655:1656	mg	1655:1656	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	7	7	theme	L-1	1532:1534	arg1	BAP					1536:1538	0.5 mg L-1 BAP	1525:1538	0.5 mg L-1 BAP	1525:1538	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	3	8	theme	optimal	658:664	arg1	drugs					666:670	The optimal drugs	654:670	The optimal drugs for sterilization of raspberry explants	654:710	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	3	8	theme	optimal	658:664	arg1	HgCl2					725:729	a) 0.1% HgCl2	717:729	a) 0.1% HgCl2 (6 min)	717:737	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	1	9	theme	clonal	162:167	arg1	micropropagation					169:184	a clonal micropropagation	160:184	a clonal micropropagation	160:184	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	1	9	theme	clonal	162:167	arg1	One					69:71	One	69:71	One	69:71	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	1	9	theme	clonal	162:167	arg1	stages					85:90	the main stages	76:90	the main stages of cryopreservation of meristematic tissues in vegetative plants	76:155	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	4	10	with	treatment	980:988	arg1	KMnO4					995:999	KMnO4	995:999	KMnO4 (30 min)	995:1008	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	4	10	with	treatment	980:988	arg1	min					1005:1007	30 min	1002:1007	30 min	1002:1007	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	8	11	theme	L-1	1658:1660	arg1	IBA					1662:1664	0.01 mg L-1 IBA	1650:1664	0.01 mg L-1 IBA	1650:1664	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	6	12	theme	media	1344:1348	arg1	compositions					1320:1331	Optimal compositions	1312:1331	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.	1312:1493	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	8	13	dep	strawberry	1606:1615	arg1	medium					1622:1627	medium	1622:1627	medium	1622:1627	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	3	14	theme	explants	703:710	arg1	sterilization					676:688	sterilization	676:688	sterilization of raspberry explants	676:710	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	5	15	theme	subsequent	1241:1250	arg1	HgCl2					1267:1271	subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1241:1309	subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1241:1309	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	9	16	theme	wild-growing	1868:1879	arg1	forms					1881:1885	wild-growing forms	1868:1885	wild-growing forms of blackcurrant, raspberry and strawberry	1868:1927	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	4	17	theme	%	904:904	arg1	"					922:922	0.1% fungicide "Topaz"	901:922	0.1% fungicide "Topaz" (30 min)	901:931	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	4	17	theme	%	904:904	arg1	min					928:930	30 min	925:930	30 min	925:930	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	5	18	theme	0,1	1262:1264	arg1	HgCl2					1267:1271	subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1241:1309	subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1241:1309	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	8	19	theme	mg	1671:1672	arg1	GA3					1678:1680	0.2 mg L-1 GA3	1667:1680	0.2 mg L-1 GA3	1667:1680	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	6	20	theme	Optimal	1312:1318	arg1	compositions					1320:1331	Optimal compositions	1312:1331	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.	1312:1493	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	5	21	theme	3	1169:1169	arg1	%					1170:1170	%	1170:1170	%	1170:1170	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	2	22	theme	growing	540:546	arg1	shoots					548:553	actively growing shoots	531:553	actively growing shoots (blackcurrants and raspberries) in May-June	531:597	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	8	23	with	strawberry	1606:1615	arg1	BAP					1645:1647	0.3 mg L-1 BAP	1634:1647	0.3 mg L-1 BAP	1634:1647	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	8	23	with	strawberry	1606:1615	arg1	chelate					1698:1704	10 mg L-1 iron chelate	1683:1704	10 mg L-1 iron chelate	1683:1704	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	8	23	with	strawberry	1606:1615	arg1	sucrose					1719:1725	30 g L-1 sucrose	1710:1725	30 g L-1 sucrose	1710:1725	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	8	23	with	strawberry	1606:1615	arg1	GA3					1678:1680	0.2 mg L-1 GA3	1667:1680	0.2 mg L-1 GA3	1667:1680	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	8	23	with	strawberry	1606:1615	arg1	IBA					1662:1664	0.01 mg L-1 IBA	1650:1664	0.01 mg L-1 IBA	1650:1664	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	8	24	theme	10	1683:1684	arg1	mg					1686:1687	mg	1686:1687	mg	1686:1687	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	6	25	theme	Skoog	1403:1407	arg1	medium					1414:1419	blackcurrant - Murashige and Skoog (MS) medium	1374:1419	blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose	1374:1492	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	7	26	theme	L-1	1563:1565	arg1	chelate					1572:1578	10 mg L-1 iron chelate	1557:1578	10 mg L-1 iron chelate	1557:1578	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	10	27	theme	germplasm	2065:2073	arg1	collection					2051:2060	a cryogenic collection	2039:2060	a cryogenic collection of germplasm based on the developed technology	2039:2107	Therefore, the aim of the research was to obtain aseptic plants, clonal micropropagation and the creation of a cryogenic collection of germplasm based on the developed technology.					
37194800	3	28	dep	«Domestos»	793:802	arg1	b					763:763	b	763:763	b	763:763	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	1	29	theme	tissues	128:134	arg1	cryopreservation					95:110	cryopreservation	95:110	cryopreservation of meristematic tissues in vegetative plants	95:155	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	8	30	theme	L-1	1689:1691	arg1	chelate					1698:1704	10 mg L-1 iron chelate	1683:1704	10 mg L-1 iron chelate	1683:1704	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	6	31	theme	Murashige	1389:1397	arg1	medium					1414:1419	blackcurrant - Murashige and Skoog (MS) medium	1374:1419	blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose	1374:1492	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	3	32	theme	%	723:723	arg1	H2O2					748:751	then 3% H2O2	740:751	then 3% H2O2 (15 min)	740:760	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	3	32	theme	%	723:723	arg1	drugs					666:670	The optimal drugs	654:670	The optimal drugs for sterilization of raspberry explants	654:710	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	3	32	theme	%	723:723	arg1	HgCl2					725:729	a) 0.1% HgCl2	717:729	a) 0.1% HgCl2 (6 min)	717:737	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	3	32	theme	%	723:723	arg1	min					734:736	6 min	732:736	6 min	732:736	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	1	33	theme	vegetative	139:148	arg1	plants					150:155	vegetative plants	139:155	vegetative plants	139:155	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	2	34	theme	buds	458:461	arg1	shoots					440:445	initiated shoots	430:445	initiated shoots of dormant buds (blackcurrants and raspberries) in January-March	430:510	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	6	35	theme	blackcurrant	1374:1385	arg1	medium					1414:1419	blackcurrant - Murashige and Skoog (MS) medium	1374:1419	blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose	1374:1492	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	1	36	from	plants	150:155	arg1	stages					85:90	the main stages	76:90	the main stages of cryopreservation of meristematic tissues in vegetative plants	76:155	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	5	37	with	treatment	1112:1120	arg1	H2O2					1172:1175	3% H2O2	1169:1175	3% H2O2 (10 min)	1169:1184	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	37	with	treatment	1112:1120	arg1	min					1163:1165	7 min	1161:1165	7 min	1161:1165	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	37	with	treatment	1112:1120	arg1	min					1139:1141	3% H2O2 10 (min); b) 1% deochlor (7 min)	1127:1166	3% H2O2 10 (min); b) 1% deochlor (7 min)	1127:1166	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	7	38	theme	L-1	1589:1591	arg1	sucrose					1593:1599	30 g L-1 sucrose	1584:1599	30 g L-1 sucrose	1584:1599	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	6	39	theme	L-1	1482:1484	arg1	glucose					1486:1492	20 g L-1 glucose	1477:1492	20 g L-1 glucose	1477:1492	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	3	40	dep	H2O2	748:751	arg1	%					746:746	%	746:746	%	746:746	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	3	41	theme	chlorine-containing	766:784	arg1	bleach					786:791	chlorine-containing bleach	766:791	chlorine-containing bleach	766:791	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	8	42	theme	L-1	1715:1717	arg1	sucrose					1719:1725	30 g L-1 sucrose	1710:1725	30 g L-1 sucrose	1710:1725	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	4	43	from	"	1023:1023	arg1	dilution					1032:1039	the dilution	1028:1039	the dilution of H2O 1:5 (20 min)	1028:1059	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	6	44	theme	L-1	1433:1435	arg1	BAP					1437:1439	0.5 mg L-1 BAP	1426:1439	0.5 mg L-1 BAP	1426:1439	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	5	45	theme	Domestos	1191:1198	arg1	"					1199:1199	c) "Domestos"	1187:1199	c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1187:1309	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	6	46	theme	20	1477:1478	arg1	g					1480:1480	g	1480:1480	g	1480:1480	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	4	47	theme	0.1	862:864	arg1	%					865:865	%	865:865	%	865:865	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	2	48	from	shoots	440:445	arg1	isolation					403:411	isolation	403:411	first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second -	395:524	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	48	from	shoots	440:445	arg1	explants					416:423	explants	416:423	explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March	416:510	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	48	from	shoots	440:445	arg1	January-March					498:510	January-March	498:510	January-March	498:510	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	48	from	shoots	440:445	arg1	first					395:399	first	395:399	first	395:399	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	48	from	shoots	440:445	arg1	periods					352:358	the optimal periods	340:358	the optimal periods for in vitro micropropagation	340:388	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	48	from	shoots	440:445	arg1	second					517:522	second	517:522	second	517:522	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	6	49	theme	0.5	1426:1428	arg1	mg					1430:1431	mg	1430:1431	mg	1430:1431	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	4	50	from	HgCl2	867:871	arg1	combination					884:894	combination	884:894	combination with 0.1% fungicide "Topaz" (30 min)	884:931	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	1	51	from	cryopreservation	95:110	arg1	plants					150:155	vegetative plants	139:155	vegetative plants	139:155	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	1	52	theme	main	80:83	arg1	stages					85:90	the main stages	76:90	the main stages of cryopreservation of meristematic tissues in vegetative plants	76:155	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	2	53	from	shoots	548:553	arg1	second					517:522	second	517:522	second	517:522	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	53	from	shoots	548:553	arg1	periods					352:358	the optimal periods	340:358	the optimal periods for in vitro micropropagation	340:388	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	53	from	shoots	548:553	arg1	first					395:399	first	395:399	first	395:399	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	53	from	shoots	548:553	arg1	isolation					403:411	isolation	403:411	first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second -	395:524	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	53	from	shoots	548:553	arg1	May-June					590:597	May-June	590:597	May-June	590:597	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	6	54	theme	medium	1414:1419	arg1	micropropagation					1354:1369	micropropagation	1354:1369	micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose	1354:1492	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	7	55	theme	10	1557:1558	arg1	mg					1560:1561	mg	1560:1561	mg	1560:1561	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	0	56	theme	berry	20:24	arg1	crops					26:30	berry crops	20:30	berry crops	20:30	Micropropagation of berry crops for creation of germplasm cryobanks.					
37194800	1	57	theme	culture	273:279	arg1	medium					281:286	the culture medium	269:286	the culture medium for micropropagation	269:307	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	5	58	dep	mg/l	1291:1294	arg1	0,20					1286:1289	0,20	1286:1289	0,20	1286:1289	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	10	59	theme	clonal	1995:2000	arg1	micropropagation					2002:2017	clonal micropropagation	1995:2017	clonal micropropagation	1995:2017	Therefore, the aim of the research was to obtain aseptic plants, clonal micropropagation and the creation of a cryogenic collection of germplasm based on the developed technology.					
37194800	9	60	dep	in	1809:1810	arg1	vitro					1812:1816	vitro	1812:1816	vitro	1812:1816	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	6	61	theme	L-1	1465:1467	arg1	IBA					1469:1471	0.1 mg L-1 IBA	1458:1471	0.1 mg L-1 IBA	1458:1471	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	4	62	from	HgCl2	942:946	arg1	combination					959:969	combination	959:969	combination with the treatment with KMnO4 (30 min)	959:1008	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	3	63	theme	1:9	827:829	arg1	dilution					811:818	the dilution	807:818	the dilution of H2O 1:9 (10 min)	807:838	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	2	64	theme	initiated	430:438	arg1	shoots					440:445	initiated shoots	430:445	initiated shoots of dormant buds (blackcurrants and raspberries) in January-March	430:510	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	10	65	theme	cryogenic	2041:2049	arg1	collection					2051:2060	a cryogenic collection	2039:2060	a cryogenic collection of germplasm based on the developed technology	2039:2107	Therefore, the aim of the research was to obtain aseptic plants, clonal micropropagation and the creation of a cryogenic collection of germplasm based on the developed technology.					
37194800	5	66	theme	%	1128:1128	arg1	H2O2					1172:1175	3% H2O2	1169:1175	3% H2O2 (10 min)	1169:1184	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	66	theme	%	1128:1128	arg1	min					1163:1165	7 min	1161:1165	7 min	1161:1165	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	66	theme	%	1128:1128	arg1	min					1139:1141	3% H2O2 10 (min); b) 1% deochlor (7 min)	1127:1166	3% H2O2 10 (min); b) 1% deochlor (7 min)	1127:1166	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	2	67	dep	first	395:399	arg1	periods					352:358	the optimal periods	340:358	the optimal periods for in vitro micropropagation	340:388	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	67	dep	first	395:399	arg1	second					517:522	second	517:522	second	517:522	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	67	dep	first	395:399	arg1	isolation					403:411	isolation	403:411	first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second -	395:524	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	67	dep	first	395:399	arg1	first					395:399	first	395:399	first	395:399	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	7	68	theme	mg	1545:1546	arg1	IBA					1552:1554	0.1 mg L-1 IBA	1541:1554	0.1 mg L-1 IBA	1541:1554	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	7	69	theme	mg	1560:1561	arg1	chelate					1572:1578	10 mg L-1 iron chelate	1557:1578	10 mg L-1 iron chelate	1557:1578	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	9	70	from	germplasm	1796:1804	arg1	hybrids					1856:1862	hybrids	1856:1862	hybrids	1856:1862	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	9	70	from	germplasm	1796:1804	arg1	forms					1881:1885	wild-growing forms	1868:1885	wild-growing forms of blackcurrant, raspberry and strawberry	1868:1927	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	9	70	from	germplasm	1796:1804	arg1	cultivars					1845:1853	66 cultivars	1842:1853	66 cultivars	1842:1853	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	8	71	theme	0.3	1634:1636	arg1	mg					1638:1639	mg	1638:1639	mg	1638:1639	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	5	72	dep	HgCl2	1267:1271	arg1	min					1307:1309	30 min	1304:1309	30 min	1304:1309	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	72	dep	HgCl2	1267:1271	arg1	КМnO4					1296:1300	then 0,20 mg/l КМnO4	1281:1300	then 0,20 mg/l КМnO4	1281:1300	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	72	dep	HgCl2	1267:1271	arg1	min					1276:1278	-7 min	1273:1278	-7 min	1273:1278	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	4	73	theme	0.1	901:903	arg1	%					904:904	%	904:904	%	904:904	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	2	74	from	January-March	498:510	arg1	shoots					440:445	initiated shoots	430:445	initiated shoots of dormant buds (blackcurrants and raspberries) in January-March	430:510	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	6	75	theme	mg	1446:1447	arg1	GA3					1453:1455	0.5 mg L-1 GA3	1442:1455	0.5 mg L-1 GA3	1442:1455	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	2	76	theme	optimal	344:350	arg1	periods					352:358	the optimal periods	340:358	the optimal periods for in vitro micropropagation	340:388	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	76	theme	optimal	344:350	arg1	second					517:522	second	517:522	second	517:522	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	76	theme	optimal	344:350	arg1	isolation					403:411	isolation	403:411	first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second -	395:524	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	76	theme	optimal	344:350	arg1	first					395:399	first	395:399	first	395:399	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	8	77	theme	L-1	1641:1643	arg1	BAP					1645:1647	0.3 mg L-1 BAP	1634:1647	0.3 mg L-1 BAP	1634:1647	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	10	78	theme	collection	2051:2060	arg1	plants					1987:1992	aseptic plants	1979:1992	aseptic plants	1979:1992	Therefore, the aim of the research was to obtain aseptic plants, clonal micropropagation and the creation of a cryogenic collection of germplasm based on the developed technology.					
37194800	10	78	theme	collection	2051:2060	arg1	micropropagation					2002:2017	clonal micropropagation	1995:2017	clonal micropropagation	1995:2017	Therefore, the aim of the research was to obtain aseptic plants, clonal micropropagation and the creation of a cryogenic collection of germplasm based on the developed technology.					
37194800	10	78	theme	collection	2051:2060	arg1	creation					2027:2034	the creation	2023:2034	the creation of a cryogenic collection of germplasm based on the developed technology	2023:2107	Therefore, the aim of the research was to obtain aseptic plants, clonal micropropagation and the creation of a cryogenic collection of germplasm based on the developed technology.					
37194800	5	79	theme	H2O	1220:1222	arg1	dilution					1208:1215	the dilution	1204:1215	the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1204:1309	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	1	80	theme	material	236:243	arg1	explants					216:223	the explants	212:223	the explants of the raw material	212:243	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	1	81	theme	raw	232:234	arg1	material					236:243	the raw material	228:243	the raw material	228:243	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	10	82	theme	developed	2088:2096	arg1	technology					2098:2107	the developed technology	2084:2107	the developed technology	2084:2107	Therefore, the aim of the research was to obtain aseptic plants, clonal micropropagation and the creation of a cryogenic collection of germplasm based on the developed technology.					
37194800	4	83	dep	blackcurrant	845:856	arg1	min					876:878	5 min	874:878	5 min	874:878	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	4	83	dep	blackcurrant	845:856	arg1	"					1023:1023	c) "Domestos"	1011:1023	blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min)	845:1059	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	4	83	dep	blackcurrant	845:856	arg1	HgCl2					942:946	0.1% HgCl2	937:946	blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min)	845:1059	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	4	83	dep	blackcurrant	845:856	arg1	min					951:953	5 min	949:953	5 min	949:953	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	4	83	dep	blackcurrant	845:856	arg1	HgCl2					867:871	0.1% HgCl2	862:871	blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min)	845:1059	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	5	84	theme	%	1084:1084	arg1	HgCl2					1086:1090	0.1% HgCl2	1081:1090	0.1% HgCl2 (6 min)	1081:1098	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	84	theme	%	1084:1084	arg1	min					1095:1097	6 min	1093:1097	6 min	1093:1097	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	85	theme	%	1265:1265	arg1	HgCl2					1267:1271	subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1241:1309	subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1241:1309	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	8	86	theme	mg	1655:1656	arg1	IBA					1662:1664	0.01 mg L-1 IBA	1650:1664	0.01 mg L-1 IBA	1650:1664	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	5	87	theme	%	1149:1149	arg1	deochlor					1151:1158	1% deochlor	1148:1158	3% H2O2 10 (min); b) 1% deochlor (7 min)	1127:1166	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	4	88	dep	HgCl2	867:871	arg1	a					859:859	a	859:859	a	859:859	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	7	89	theme	0.1	1541:1543	arg1	mg					1545:1546	mg	1545:1546	mg	1545:1546	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	7	90	theme	0.5	1525:1527	arg1	mg					1529:1530	mg	1529:1530	mg	1529:1530	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	4	91	theme	Domestos	1015:1022	arg1	"					1023:1023	c) "Domestos"	1011:1023	blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min)	845:1059	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	8	92	theme	0.2	1667:1669	arg1	mg					1671:1672	mg	1671:1672	mg	1671:1672	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	4	93	theme	fungicide	906:914	arg1	"					922:922	0.1% fungicide "Topaz"	901:922	0.1% fungicide "Topaz" (30 min)	901:931	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	4	93	theme	fungicide	906:914	arg1	min					928:930	30 min	925:930	30 min	925:930	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	6	94	theme	culture	1336:1342	arg1	media					1344:1348	culture media	1336:1348	culture media	1336:1348	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	5	95	theme	treatment	1252:1260	arg1	HgCl2					1267:1271	subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1241:1309	subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1241:1309	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	4	96	dep	HgCl2	942:946	arg1	b					934:934	b	934:934	b	934:934	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	4	97	with	combination	959:969	arg1	treatment					980:988	the treatment	976:988	the treatment with KMnO4 (30 min)	976:1008	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	8	98	theme	L-1	1674:1676	arg1	GA3					1678:1680	0.2 mg L-1 GA3	1667:1680	0.2 mg L-1 GA3	1667:1680	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	2	99	from	buds	458:461	arg1	January-March					498:510	January-March	498:510	January-March	498:510	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	4	100	theme	Topaz	917:921	arg1	"					922:922	0.1% fungicide "Topaz"	901:922	0.1% fungicide "Topaz" (30 min)	901:931	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	4	100	theme	Topaz	917:921	arg1	min					928:930	30 min	925:930	30 min	925:930	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	3	101	theme	raspberry	693:701	arg1	explants					703:710	raspberry explants	693:710	raspberry explants	693:710	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	7	102	theme	mg	1529:1530	arg1	BAP					1536:1538	0.5 mg L-1 BAP	1525:1538	0.5 mg L-1 BAP	1525:1538	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	2	103	dep	shoots	548:553	arg1	blackcurrants					556:568	blackcurrants	556:568	blackcurrants	556:568	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	103	dep	shoots	548:553	arg1	raspberries					574:584	raspberries	574:584	raspberries	574:584	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	4	104	theme	%	940:940	arg1	HgCl2					942:946	0.1% HgCl2	937:946	blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min)	845:1059	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	4	104	theme	%	940:940	arg1	min					951:953	5 min	949:953	5 min	949:953	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	5	105	theme	%	1170:1170	arg1	H2O2					1172:1175	3% H2O2	1169:1175	3% H2O2 (10 min)	1169:1184	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	105	theme	%	1170:1170	arg1	min					1181:1183	10 min	1178:1183	10 min	1178:1183	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	105	theme	%	1170:1170	arg1	min					1139:1141	3% H2O2 10 (min); b) 1% deochlor (7 min)	1127:1166	3% H2O2 10 (min); b) 1% deochlor (7 min)	1127:1166	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	4	106	theme	H2O	1044:1046	arg1	dilution					1032:1039	the dilution	1028:1039	the dilution of H2O 1:5 (20 min)	1028:1059	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	1	107	theme	cryopreservation	95:110	arg1	stages					85:90	the main stages	76:90	the main stages of cryopreservation of meristematic tissues in vegetative plants	76:155	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	9	108	theme	raspberry	1904:1912	arg1	hybrids					1856:1862	hybrids	1856:1862	hybrids	1856:1862	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	9	108	theme	raspberry	1904:1912	arg1	forms					1881:1885	wild-growing forms	1868:1885	wild-growing forms of blackcurrant, raspberry and strawberry	1868:1927	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	9	108	theme	raspberry	1904:1912	arg1	cultivars					1845:1853	66 cultivars	1842:1853	66 cultivars	1842:1853	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	8	109	theme	mg	1686:1687	arg1	chelate					1698:1704	10 mg L-1 iron chelate	1683:1704	10 mg L-1 iron chelate	1683:1704	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	1	110	theme	meristematic	115:126	arg1	tissues					128:134	meristematic tissues	115:134	meristematic tissues	115:134	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	9	111	theme	strawberry	1918:1927	arg1	hybrids					1856:1862	hybrids	1856:1862	hybrids	1856:1862	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	9	111	theme	strawberry	1918:1927	arg1	forms					1881:1885	wild-growing forms	1868:1885	wild-growing forms of blackcurrant, raspberry and strawberry	1868:1927	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	9	111	theme	strawberry	1918:1927	arg1	cultivars					1845:1853	66 cultivars	1842:1853	66 cultivars	1842:1853	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	8	112	theme	iron	1693:1696	arg1	chelate					1698:1704	10 mg L-1 iron chelate	1683:1704	10 mg L-1 iron chelate	1683:1704	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	7	113	dep	medium	1513:1518	arg1	-MS					1509:1511	raspberry -MS	1499:1511	raspberry -MS	1499:1511	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	2	114	from	isolation	403:411	arg1	shoots					440:445	initiated shoots	430:445	initiated shoots of dormant buds (blackcurrants and raspberries) in January-March	430:510	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	115	from	mustache	616:623	arg1	periods					352:358	the optimal periods	340:358	the optimal periods for in vitro micropropagation	340:388	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	115	from	mustache	616:623	arg1	second					517:522	second	517:522	second	517:522	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	115	from	mustache	616:623	arg1	July-August					641:651	July-August	641:651	July-August	641:651	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	115	from	mustache	616:623	arg1	isolation					403:411	isolation	403:411	first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second -	395:524	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	115	from	mustache	616:623	arg1	first					395:399	first	395:399	first	395:399	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	8	116	theme	g	1713:1713	arg1	sucrose					1719:1725	30 g L-1 sucrose	1710:1725	30 g L-1 sucrose	1710:1725	For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	2	117	theme	dormant	450:456	arg1	buds					458:461	dormant buds	450:461	dormant buds (blackcurrants and raspberries) in January-March	450:510	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	10	118	theme	research	1956:1963	arg1	aim					1945:1947	the aim	1941:1947	the aim of the research	1941:1963	Therefore, the aim of the research was to obtain aseptic plants, clonal micropropagation and the creation of a cryogenic collection of germplasm based on the developed technology.					
37194800	2	119	theme	formed	609:614	arg1	strawberry					626:635	strawberry	626:635	strawberry	626:635	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	119	theme	formed	609:614	arg1	mustache					616:623	the formed mustache	605:623	the formed mustache (strawberry) in July-August	605:651	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	7	120	theme	L-1	1548:1550	arg1	IBA					1552:1554	0.1 mg L-1 IBA	1541:1554	0.1 mg L-1 IBA	1541:1554	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	4	121	with	combination	884:894	arg1	"					922:922	0.1% fungicide "Topaz"	901:922	0.1% fungicide "Topaz" (30 min)	901:931	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	4	121	with	combination	884:894	arg1	min					928:930	30 min	925:930	30 min	925:930	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	3	122	dep	%	746:746	arg1	3					745:745	3	745:745	3	745:745	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	5	123	theme	3	1127:1127	arg1	%					1128:1128	%	1128:1128	%	1128:1128	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	6	124	theme	g	1480:1480	arg1	glucose					1486:1492	20 g L-1 glucose	1477:1492	20 g L-1 glucose	1477:1492	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	9	125	theme	tissues	1831:1837	arg1	germplasm					1796:1804	the germplasm	1792:1804	the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry	1792:1927	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	10	126	theme	aseptic	1979:1985	arg1	plants					1987:1992	aseptic plants	1979:1992	aseptic plants	1979:1992	Therefore, the aim of the research was to obtain aseptic plants, clonal micropropagation and the creation of a cryogenic collection of germplasm based on the developed technology.					
37194800	9	127	theme	blackcurrant	1890:1901	arg1	hybrids					1856:1862	hybrids	1856:1862	hybrids	1856:1862	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	9	127	theme	blackcurrant	1890:1901	arg1	forms					1881:1885	wild-growing forms	1868:1885	wild-growing forms of blackcurrant, raspberry and strawberry	1868:1927	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	9	127	theme	blackcurrant	1890:1901	arg1	cultivars					1845:1853	66 cultivars	1842:1853	66 cultivars	1842:1853	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	6	128	theme	mg	1430:1431	arg1	BAP					1437:1439	0.5 mg L-1 BAP	1426:1439	0.5 mg L-1 BAP	1426:1439	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	2	129	theme	in	364:365	arg1	micropropagation					373:388	in vitro micropropagation	364:388	in vitro micropropagation	364:388	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	6	130	theme	0.1	1458:1460	arg1	mg					1462:1463	mg	1462:1463	mg	1462:1463	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	5	131	dep	КМnO4	1296:1300	arg1	mg/l					1291:1294	mg/l	1291:1294	mg/l	1291:1294	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	9	132	theme	meristematic	1818:1829	arg1	tissues					1831:1837	in vitro meristematic tissues	1809:1837	in vitro meristematic tissues	1809:1837	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	0	133	theme	crops	26:30	arg1	Micropropagation					0:15	Micropropagation	0:15	Micropropagation of berry crops for creation of germplasm	0:56	Micropropagation of berry crops for creation of germplasm cryobanks.					
37194800	1	134	theme	stages	85:90	arg1	One					69:71	One	69:71	One	69:71	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	1	134	theme	stages	85:90	arg1	micropropagation					169:184	a clonal micropropagation	160:184	a clonal micropropagation	160:184	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	1	134	theme	stages	85:90	arg1	stages					85:90	the main stages	76:90	the main stages of cryopreservation of meristematic tissues in vegetative plants	76:155	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	4	135	theme	%	865:865	arg1	min					876:878	5 min	874:878	5 min	874:878	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	4	135	theme	%	865:865	arg1	HgCl2					867:871	0.1% HgCl2	862:871	blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min)	845:1059	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	7	136	theme	iron	1567:1570	arg1	chelate					1572:1578	10 mg L-1 iron chelate	1557:1578	10 mg L-1 iron chelate	1557:1578	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	5	137	theme	c	1187:1187	arg1	"					1199:1199	c) "Domestos"	1187:1199	c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1187:1309	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	7	138	with	medium	1513:1518	arg1	chelate					1572:1578	10 mg L-1 iron chelate	1557:1578	10 mg L-1 iron chelate	1557:1578	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	7	138	with	medium	1513:1518	arg1	sucrose					1593:1599	30 g L-1 sucrose	1584:1599	30 g L-1 sucrose	1584:1599	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	7	138	with	medium	1513:1518	arg1	IBA					1552:1554	0.1 mg L-1 IBA	1541:1554	0.1 mg L-1 IBA	1541:1554	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	7	138	with	medium	1513:1518	arg1	BAP					1536:1538	0.5 mg L-1 BAP	1525:1538	0.5 mg L-1 BAP	1525:1538	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	7	139	theme	raspberry	1499:1507	arg1	-MS					1509:1511	raspberry -MS	1499:1511	raspberry -MS	1499:1511	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	6	140	with	medium	1414:1419	arg1	glucose					1486:1492	20 g L-1 glucose	1477:1492	20 g L-1 glucose	1477:1492	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	6	140	with	medium	1414:1419	arg1	IBA					1469:1471	0.1 mg L-1 IBA	1458:1471	0.1 mg L-1 IBA	1458:1471	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	6	140	with	medium	1414:1419	arg1	GA3					1453:1455	0.5 mg L-1 GA3	1442:1455	0.5 mg L-1 GA3	1442:1455	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	6	140	with	medium	1414:1419	arg1	BAP					1437:1439	0.5 mg L-1 BAP	1426:1439	0.5 mg L-1 BAP	1426:1439	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	5	141	dep	followed	1100:1107	arg1	a					1078:1078	a	1078:1078	a	1078:1078	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	141	dep	followed	1100:1107	arg1	"					1199:1199	c) "Domestos"	1187:1199	c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min	1187:1309	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	4	142	theme	c	1011:1011	arg1	"					1023:1023	c) "Domestos"	1011:1023	blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min)	845:1059	For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide "Topaz" (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) "Domestos" in the dilution of H2O 1:5 (20 min).					
37194800	6	143	theme	mg	1462:1463	arg1	IBA					1469:1471	0.1 mg L-1 IBA	1458:1471	0.1 mg L-1 IBA	1458:1471	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	0	144	theme	germplasm	48:56	arg1	creation					36:43	creation	36:43	creation of germplasm	36:56	Micropropagation of berry crops for creation of germplasm cryobanks.					
37194800	2	145	dep	buds	458:461	arg1	raspberries					482:492	raspberries	482:492	raspberries	482:492	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	145	dep	buds	458:461	arg1	blackcurrants					464:476	blackcurrants	464:476	blackcurrants	464:476	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	5	146	theme	H2O2	1130:1133	arg1	H2O2					1172:1175	3% H2O2	1169:1175	3% H2O2 (10 min)	1169:1184	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	146	theme	H2O2	1130:1133	arg1	min					1163:1165	7 min	1161:1165	7 min	1161:1165	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	5	146	theme	H2O2	1130:1133	arg1	min					1139:1141	3% H2O2 10 (min); b) 1% deochlor (7 min)	1127:1166	3% H2O2 10 (min); b) 1% deochlor (7 min)	1127:1166	For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) "Domestos" in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min.					
37194800	1	147	from	stages	85:90	arg1	plants					150:155	vegetative plants	139:155	vegetative plants	139:155	One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation.					
37194800	6	148	theme	0.5	1442:1444	arg1	mg					1446:1447	mg	1446:1447	mg	1446:1447	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	3	149	theme	H2O	823:825	arg1	1:9					827:829	H2O 1:9	823:829	H2O 1:9 (10 min)	823:838	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	3	149	theme	H2O	823:825	arg1	min					835:837	10 min	832:837	10 min	832:837	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
37194800	7	150	theme	g	1587:1587	arg1	sucrose					1593:1599	30 g L-1 sucrose	1584:1599	30 g L-1 sucrose	1584:1599	For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose.					
37194800	6	151	theme	L-1	1449:1451	arg1	GA3					1453:1455	0.5 mg L-1 GA3	1442:1455	0.5 mg L-1 GA3	1442:1455	Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose.					
37194800	9	152	theme	in	1809:1810	arg1	tissues					1831:1837	in vitro meristematic tissues	1809:1837	in vitro meristematic tissues	1809:1837	Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry.					
37194800	2	153	dep	in	364:365	arg1	vitro					367:371	vitro	367:371	vitro	367:371	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	154	theme	explants	416:423	arg1	periods					352:358	the optimal periods	340:358	the optimal periods for in vitro micropropagation	340:388	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	154	theme	explants	416:423	arg1	second					517:522	second	517:522	second	517:522	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	154	theme	explants	416:423	arg1	isolation					403:411	isolation	403:411	first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second -	395:524	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	2	154	theme	explants	416:423	arg1	first					395:399	first	395:399	first	395:399	As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August.					
37194800	3	155	theme	0.1	720:722	arg1	%					723:723	%	723:723	%	723:723	The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min).					
35358954	6	0	theme	P-M	1092:1094	arg1	biocompatibility					1072:1087	best biocompatibility	1067:1087	best biocompatibility of P-M and P-Ag@M membranes	1067:1115	Furthermore, best biocompatibility of P-M and P-Ag@M membranes compare to PLLA membrane motivated us to further explore its application in biomedical materials.					
35358954	0	1	from	assembly	4:11	arg1	substrate					46:54	PLLA substrate	41:54	PLLA substrate	41:54	LBL assembly of Ag@Ti3C2Tand chitosan on PLLA substrate to enhance antibacterial and biocompatibility.					
35358954	4	2	theme	M	799:799	arg1	membrane					801:808	P-Ag@M membrane	794:808	P-Ag@M membrane	794:808	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	5	3	theme	nm	967:968	arg1	radiation					990:998	808 nm near-infrared laser radiation	963:998	808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect	963:1051	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	3	4	theme	charged	519:525	arg1	MXene					530:534	negatively charged Ag@MXene	508:534	negatively charged Ag@MXene	508:534	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	3	5	theme	Ag	527:528	arg1	MXene					530:534	negatively charged Ag@MXene	508:534	negatively charged Ag@MXene	508:534	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	6	6	theme	@	1104:1104	arg1	membranes					1107:1115	@M membranes	1104:1115	@M membranes	1104:1115	Furthermore, best biocompatibility of P-M and P-Ag@M membranes compare to PLLA membrane motivated us to further explore its application in biomedical materials.					
35358954	5	7	with	radiation	990:998	arg1	effect					1046:1051	a synergistic photothermal antibacterial effect	1005:1051	a synergistic photothermal antibacterial effect	1005:1051	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	4	8	theme	12.1	745:748	arg1	±					743:743	±	743:743	±	743:743	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	4	9	theme	fluorescein	627:637	arg1	chitosan					663:670	fluorescein isothiocyanate-labelled chitosan	627:670	fluorescein isothiocyanate-labelled chitosan	627:670	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	1	10	theme	non-toxic	134:142	arg1	acid					117:120	Poly L-lactic acid	103:120	Poly L-lactic acid (PLLA)	103:127	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	1	10	theme	non-toxic	134:142	arg1	polymer					170:176	a non-toxic, biocompatible degradable polymer	132:176	polymer	170:176	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	3	11	dep	method	376:381	arg1	prepare					386:392	prepare	386:392	to prepare a composite multilayer membrane	383:424	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	6	12	theme	PLLA	1128:1131	arg1	membrane					1133:1140	PLLA membrane	1128:1140	PLLA membrane motivated us to further explore its application in biomedical materials	1128:1212	Furthermore, best biocompatibility of P-M and P-Ag@M membranes compare to PLLA membrane motivated us to further explore its application in biomedical materials.					
35358954	5	13	dep	%	865:865	arg1	againstEscherichia					902:919	growth inhibition ratio againstEscherichia	878:919	growth inhibition ratio againstEscherichia	878:919	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	6	14	theme	best	1067:1070	arg1	biocompatibility					1072:1087	best biocompatibility	1067:1087	best biocompatibility of P-M and P-Ag@M membranes	1067:1115	Furthermore, best biocompatibility of P-M and P-Ag@M membranes compare to PLLA membrane motivated us to further explore its application in biomedical materials.					
35358954	4	15	theme	assembly	594:601	arg1	process					603:609	The assembly process	590:609	The assembly process	590:609	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	4	16	theme	@	798:798	arg1	membrane					801:808	P-Ag@M membrane	794:808	P-Ag@M membrane	794:808	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	2	17	theme	materials	294:302	arg1	use					276:278	the use	272:278	the use of biomedical materials	272:302	However, it faces challenges in the use of biomedical materials because of its intolerance to bacteria.					
35358954	1	18	theme	biocompatible	145:157	arg1	acid					117:120	Poly L-lactic acid	103:120	Poly L-lactic acid (PLLA)	103:127	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	1	18	theme	biocompatible	145:157	arg1	polymer					170:176	a non-toxic, biocompatible degradable polymer	132:176	polymer	170:176	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	0	19	theme	LBL	0:2	arg1	assembly					4:11	LBL assembly	0:11	LBL assembly of Ag@Ti3C2Tand chitosan on PLLA substrate	0:54	LBL assembly of Ag@Ti3C2Tand chitosan on PLLA substrate to enhance antibacterial and biocompatibility.					
35358954	4	20	theme	P-Ag	794:797	arg1	membrane					801:808	P-Ag@M membrane	794:808	P-Ag@M membrane	794:808	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	2	21	theme	biomedical	283:292	arg1	materials					294:302	biomedical materials	283:302	biomedical materials	283:302	However, it faces challenges in the use of biomedical materials because of its intolerance to bacteria.					
35358954	1	22	theme	degradable	159:168	arg1	acid					117:120	Poly L-lactic acid	103:120	Poly L-lactic acid (PLLA)	103:127	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	1	22	theme	degradable	159:168	arg1	polymer					170:176	a non-toxic, biocompatible degradable polymer	132:176	polymer	170:176	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	0	23	theme	Ag	16:17	arg1	chitosan					29:36	Ag@Ti3C2Tand chitosan	16:36	Ag@Ti3C2Tand chitosan	16:36	LBL assembly of Ag@Ti3C2Tand chitosan on PLLA substrate to enhance antibacterial and biocompatibility.					
35358954	3	24	theme	@	529:529	arg1	MXene					530:534	negatively charged Ag@MXene	508:534	negatively charged Ag@MXene	508:534	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	6	25	dep	P-M	1092:1094	arg1	membranes					1107:1115	@M membranes	1104:1115	@M membranes	1104:1115	Furthermore, best biocompatibility of P-M and P-Ag@M membranes compare to PLLA membrane motivated us to further explore its application in biomedical materials.					
35358954	4	26	theme	multilayer	705:714	arg1	nm					787:788	460.5 ± 26.5 nm	774:788	460.5 ± 26.5 nm for P-Ag@M membrane	774:808	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	4	26	theme	multilayer	705:714	arg1	nm					750:751	210.0 ± 12.1 nm	737:751	210.0 ± 12.1 nm for P-M membrane	737:768	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	4	26	theme	multilayer	705:714	arg1	thickness					680:688	the thickness	676:688	the thickness of the coating multilayer	676:714	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	5	27	theme	growth	878:883	arg1	againstEscherichia					902:919	growth inhibition ratio againstEscherichia	878:919	growth inhibition ratio againstEscherichia	878:919	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	5	28	theme	ratio	896:900	arg1	againstEscherichia					902:919	growth inhibition ratio againstEscherichia	878:919	growth inhibition ratio againstEscherichia	878:919	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	1	29	theme	material	178:185	arg1	acid					117:120	Poly L-lactic acid	103:120	Poly L-lactic acid (PLLA)	103:127	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	1	29	theme	material	178:185	arg1	polymer					170:176	a non-toxic, biocompatible degradable polymer	132:176	polymer	170:176	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	0	30	theme	Ti3C2Tand	19:27	arg1	chitosan					29:36	Ag@Ti3C2Tand chitosan	16:36	Ag@Ti3C2Tand chitosan	16:36	LBL assembly of Ag@Ti3C2Tand chitosan on PLLA substrate to enhance antibacterial and biocompatibility.					
35358954	5	31	theme	laser	984:988	arg1	radiation					990:998	808 nm near-infrared laser radiation	963:998	808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect	963:1051	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	4	32	theme	460.5	774:778	arg1	±					780:780	±	780:780	±	780:780	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	3	33	theme	composite	396:404	arg1	membrane					417:424	a composite multilayer membrane	394:424	a composite multilayer membrane	394:424	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	0	34	theme	@	18:18	arg1	chitosan					29:36	Ag@Ti3C2Tand chitosan	16:36	Ag@Ti3C2Tand chitosan	16:36	LBL assembly of Ag@Ti3C2Tand chitosan on PLLA substrate to enhance antibacterial and biocompatibility.					
35358954	1	35	with	material	178:185	arg1	properties					213:222	excellent mechanical properties	192:222	excellent mechanical properties after moulding	192:237	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	3	36	used	used	445:448	arg2	membrane					432:439	PLLA membrane	427:439	PLLA membrane	427:439	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	3	36	used	used	445:448	arg2	substrates					453:462	substrates	453:462	substrates	453:462	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	1	37	theme	excellent	192:200	arg1	properties					213:222	excellent mechanical properties	192:222	excellent mechanical properties after moulding	192:237	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	3	38	theme	easy-to-operate	360:374	arg1	method					376:381	an easy-to-operate method	357:381	an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method	357:587	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	5	39	theme	surface	815:821	arg1	multilayers					838:848	The surface self-assembled multilayers	811:848	The surface self-assembled multilayers	811:848	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	3	40	theme	multilayer	406:415	arg1	membrane					417:424	a composite multilayer membrane	394:424	a composite multilayer membrane	394:424	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	4	41	theme	26.5	782:785	arg1	±					780:780	±	780:780	±	780:780	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	6	42	theme	P-Ag	1100:1103	arg1	biocompatibility					1072:1087	best biocompatibility	1067:1087	best biocompatibility of P-M and P-Ag@M membranes	1067:1115	Furthermore, best biocompatibility of P-M and P-Ag@M membranes compare to PLLA membrane motivated us to further explore its application in biomedical materials.					
35358954	1	43	theme	Poly	103:106	arg1	acid					117:120	Poly L-lactic acid	103:120	Poly L-lactic acid (PLLA)	103:127	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	1	43	theme	Poly	103:106	arg1	polymer					170:176	a non-toxic, biocompatible degradable polymer	132:176	polymer	170:176	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	1	43	theme	Poly	103:106	arg1	PLLA					123:126	PLLA	123:126	PLLA	123:126	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	0	44	theme	chitosan	29:36	arg1	assembly					4:11	LBL assembly	0:11	LBL assembly of Ag@Ti3C2Tand chitosan on PLLA substrate	0:54	LBL assembly of Ag@Ti3C2Tand chitosan on PLLA substrate to enhance antibacterial and biocompatibility.					
35358954	5	45	theme	photothermal	1019:1030	arg1	effect					1046:1051	a synergistic photothermal antibacterial effect	1005:1051	a synergistic photothermal antibacterial effect	1005:1051	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	5	46	theme	synergistic	1007:1017	arg1	effect					1046:1051	a synergistic photothermal antibacterial effect	1005:1051	a synergistic photothermal antibacterial effect	1005:1051	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	1	47	theme	L-lactic	108:115	arg1	acid					117:120	Poly L-lactic acid	103:120	Poly L-lactic acid (PLLA)	103:127	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	1	47	theme	L-lactic	108:115	arg1	polymer					170:176	a non-toxic, biocompatible degradable polymer	132:176	polymer	170:176	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	1	47	theme	L-lactic	108:115	arg1	PLLA					123:126	PLLA	123:126	PLLA	123:126	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	4	48	theme	P-M	757:759	arg1	membrane					761:768	P-M membrane	757:768	P-M membrane	757:768	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	5	49	theme	antibacterial	1032:1044	arg1	effect					1046:1051	a synergistic photothermal antibacterial effect	1005:1051	a synergistic photothermal antibacterial effect	1005:1051	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	5	50	theme	808	963:965	arg1	nm					967:968	nm	967:968	nm	967:968	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	3	51	theme	layer-by-layer	561:574	arg1	method					582:587	the layer-by-layer (LBL) method	557:587	the layer-by-layer (LBL) method	557:587	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	4	52	theme	coating	697:703	arg1	multilayer					705:714	the coating multilayer	693:714	the coating multilayer	693:714	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	5	53	theme	inhibition	885:894	arg1	againstEscherichia					902:919	growth inhibition ratio againstEscherichia	878:919	growth inhibition ratio againstEscherichia	878:919	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	1	54	theme	mechanical	202:211	arg1	properties					213:222	excellent mechanical properties	192:222	excellent mechanical properties after moulding	192:237	Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding.					
35358954	6	55	theme	biomedical	1193:1202	arg1	materials					1204:1212	biomedical materials	1193:1212	biomedical materials	1193:1212	Furthermore, best biocompatibility of P-M and P-Ag@M membranes compare to PLLA membrane motivated us to further explore its application in biomedical materials.					
35358954	4	56	theme	210.0	737:741	arg1	±					743:743	±	743:743	±	743:743	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	5	57	theme	self-assembled	823:836	arg1	multilayers					838:848	The surface self-assembled multilayers	811:848	The surface self-assembled multilayers	811:848	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	6	58	theme	M	1105:1105	arg1	membranes					1107:1115	@M membranes	1104:1115	@M membranes	1104:1115	Furthermore, best biocompatibility of P-M and P-Ag@M membranes compare to PLLA membrane motivated us to further explore its application in biomedical materials.					
35358954	3	59	theme	PLLA	427:430	arg1	substrates					453:462	substrates	453:462	substrates	453:462	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	3	59	theme	PLLA	427:430	arg1	membrane					432:439	PLLA membrane	427:439	PLLA membrane	427:439	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	5	60	theme	near-infrared	970:982	arg1	radiation					990:998	808 nm near-infrared laser radiation	963:998	808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect	963:1051	The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio againstEscherichia coliandStaphylococcus aureusstrains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect.					
35358954	3	61	theme	charged	487:493	arg1	chitosan					495:502	positively charged chitosan	476:502	positively charged chitosan	476:502	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	0	62	theme	PLLA	41:44	arg1	substrate					46:54	PLLA substrate	41:54	PLLA substrate	41:54	LBL assembly of Ag@Ti3C2Tand chitosan on PLLA substrate to enhance antibacterial and biocompatibility.					
35358954	3	63	theme	LBL	577:579	arg1	method					582:587	the layer-by-layer (LBL) method	557:587	the layer-by-layer (LBL) method	557:587	Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method.					
35358954	4	64	theme	isothiocyanate-labelled	639:661	arg1	chitosan					663:670	fluorescein isothiocyanate-labelled chitosan	627:670	fluorescein isothiocyanate-labelled chitosan	627:670	The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane.					
35358954	6	65	from	application	1178:1188	arg1	materials					1204:1212	biomedical materials	1193:1212	biomedical materials	1193:1212	Furthermore, best biocompatibility of P-M and P-Ag@M membranes compare to PLLA membrane motivated us to further explore its application in biomedical materials.					
36087975	0	0	theme	humidity	98:105	arg1	response					107:114	ultra-fast humidity response	87:114	ultra-fast humidity response	87:114	Nature-inspired construction of iridescent CNC/Nano-lignin films for UV resistance and ultra-fast humidity response.					
36087975	3	1	dep	induced	398:404	arg1	formed					427:432	formed	427:432	induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs	398:573	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	1	2	theme	renewable	178:186	arg1	biomolecules					188:199	sustainable and renewable biomolecules	162:199	sustainable and renewable biomolecules	162:199	Exploration of functional materials based on sustainable and renewable biomolecules has been of much interest.					
36087975	0	3	theme	ultra-fast	87:96	arg1	response					107:114	ultra-fast humidity response	87:114	ultra-fast humidity response	87:114	Nature-inspired construction of iridescent CNC/Nano-lignin films for UV resistance and ultra-fast humidity response.					
36087975	5	4	theme	contact	851:857	arg1	angle					859:863	the contact angle	847:863	the contact angle of 75°	847:870	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	4	5	theme	layer	618:622	arg1	gaps					624:627	expanding layer gaps	608:627	expanding layer gaps	608:627	Decreased crystallite sizes and expanding layer gaps indicated the homogeneous distribution and hydrophobic interactions between CNCs and LNPs.					
36087975	6	6	theme	optical	966:972	arg1	films					974:978	The proposed optical films	953:978	The proposed optical films	953:978	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	5	7	theme	Distinguished	720:732	arg1	capabilities					748:759	Distinguished UV absorption capabilities	720:759	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75°	720:870	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	5	8	theme	UVB	802:804	arg1	region					806:811	UVB region	802:811	UVB region	802:811	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	6	9	theme	proposed	957:964	arg1	films					974:978	The proposed optical films	953:978	The proposed optical films	953:978	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	5	10	theme	UV	734:735	arg1	capabilities					748:759	Distinguished UV absorption capabilities	720:759	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75°	720:870	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	2	11	theme	nanoparticles	318:330	arg1	incorporation					284:296	incorporation	284:296	incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs)	284:383	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	3	12	theme	helix	518:522	arg1	pitches					524:530	enlarged helix pitches	509:530	enlarged helix pitches	509:530	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	2	13	theme	lignin	311:316	arg1	nanoparticles					318:330	bio-based lignin nanoparticles	301:330	bio-based lignin nanoparticles (LNPs)	301:337	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	2	13	theme	lignin	311:316	arg1	LNPs					333:336	LNPs	333:336	LNPs	333:336	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	5	14	theme	75°	868:870	arg1	angle					859:863	the contact angle	847:863	the contact angle of 75°	847:870	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	3	15	dep	formed	427:432	arg1	layered					447:453	layered	447:453	layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs	447:573	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	3	15	dep	formed	427:432	arg1	oriented					434:441	oriented	434:441	oriented	434:441	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	2	16	theme	bio-based	301:309	arg1	nanoparticles					318:330	bio-based lignin nanoparticles	301:330	bio-based lignin nanoparticles (LNPs)	301:337	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	2	16	theme	bio-based	301:309	arg1	LNPs					333:336	LNPs	333:336	LNPs	333:336	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	6	17	theme	sensing	1151:1157	arg1	stimuli					1143:1149	stimuli	1143:1149	stimuli sensing, anti-counterfeiting	1143:1178	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	5	18	dep	%	774:774	arg1	90 					771:773	90 	771:773	90 	771:773	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	5	19	with	capabilities	748:759	arg1	angle					859:863	the contact angle	847:863	the contact angle of 75°	847:870	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	5	19	with	capabilities	748:759	arg1	capabilities					786:797	over 90 % shielding capabilities	766:797	over 90 % shielding capabilities in UVB region and increased hydrophobicity	766:840	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	2	20	theme	chiral	344:349	arg1	crystals					369:376	chiral nematic cellulose crystals	344:376	chiral nematic cellulose crystals (CNCs)	344:383	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	2	20	theme	chiral	344:349	arg1	CNCs					379:382	CNCs	379:382	CNCs	379:382	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	5	21	theme	shielding	776:784	arg1	capabilities					786:797	over 90 % shielding capabilities	766:797	over 90 % shielding capabilities in UVB region and increased hydrophobicity	766:840	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	0	22	theme	Nature-inspired	0:14	arg1	construction					16:27	Nature-inspired construction	0:27	Nature-inspired construction of iridescent CNC/Nano-lignin films for UV resistance and ultra-fast humidity response.	0:115	Nature-inspired construction of iridescent CNC/Nano-lignin films for UV resistance and ultra-fast humidity response.					
36087975	6	23	from	potentials	1109:1118	arg1	fields					1128:1133	many fields	1123:1133	many fields such as stimuli sensing, anti-counterfeiting and wearable devices	1123:1199	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	6	23	from	potentials	1109:1118	arg1	stimuli					1143:1149	stimuli	1143:1149	stimuli sensing, anti-counterfeiting	1143:1178	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	6	23	from	potentials	1109:1118	arg1	devices					1193:1199	wearable devices	1184:1199	wearable devices	1184:1199	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	1	24	theme	much	213:216	arg1	interest					218:225	much interest	213:225	much interest	213:225	Exploration of functional materials based on sustainable and renewable biomolecules has been of much interest.					
36087975	0	25	theme	iridescent	32:41	arg1	films					59:63	iridescent CNC/Nano-lignin films	32:63	iridescent CNC/Nano-lignin films	32:63	Nature-inspired construction of iridescent CNC/Nano-lignin films for UV resistance and ultra-fast humidity response.					
36087975	4	26	theme	crystallite	586:596	arg1	sizes					598:602	Decreased crystallite sizes	576:602	Decreased crystallite sizes	576:602	Decreased crystallite sizes and expanding layer gaps indicated the homogeneous distribution and hydrophobic interactions between CNCs and LNPs.					
36087975	6	27	theme	many	1123:1126	arg1	fields					1128:1133	many fields	1123:1133	many fields such as stimuli sensing, anti-counterfeiting and wearable devices	1123:1199	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	6	27	theme	many	1123:1126	arg1	stimuli					1143:1149	stimuli	1143:1149	stimuli sensing, anti-counterfeiting	1143:1178	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	6	27	theme	many	1123:1126	arg1	devices					1193:1199	wearable devices	1184:1199	wearable devices	1184:1199	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	3	28	theme	films	498:502	arg1	structure					455:463	structure	455:463	structure of the nanocomposites iridescent films with enlarged helix pitches	455:530	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	4	29	theme	expanding	608:616	arg1	gaps					624:627	expanding layer gaps	608:627	expanding layer gaps	608:627	Decreased crystallite sizes and expanding layer gaps indicated the homogeneous distribution and hydrophobic interactions between CNCs and LNPs.					
36087975	6	30	dep	sensing	1151:1157	arg1	anti-counterfeiting					1160:1178	anti-counterfeiting	1160:1178	anti-counterfeiting	1160:1178	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	3	31	theme	higher	552:557	arg1	LNPs					570:573	LNPs	570:573	LNPs	570:573	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	3	31	theme	higher	552:557	arg1	amounts					559:565	higher amounts	552:565	higher amounts of LNPs	552:573	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	0	32	theme	films	59:63	arg1	construction					16:27	Nature-inspired construction	0:27	Nature-inspired construction of iridescent CNC/Nano-lignin films for UV resistance and ultra-fast humidity response.	0:115	Nature-inspired construction of iridescent CNC/Nano-lignin films for UV resistance and ultra-fast humidity response.					
36087975	6	33	theme	all-natural	1031:1041	arg1	components					1043:1052	all-natural components	1031:1052	all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices	1031:1199	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	3	34	with	films	498:502	arg1	pitches					524:530	enlarged helix pitches	509:530	enlarged helix pitches	509:530	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	5	35	theme	due	910:912	arg1	films					904:908	the composite films	890:908	the composite films due to the presence of hydrophobic lignin	890:950	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	5	36	theme	hydrophobic	933:943	arg1	lignin					945:950	hydrophobic lignin	933:950	hydrophobic lignin	933:950	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	3	37	theme	nanocomposites	472:485	arg1	films					498:502	the nanocomposites iridescent films	468:502	the nanocomposites iridescent films with enlarged helix pitches	468:530	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	0	38	theme	CNC/Nano-lignin	43:57	arg1	films					59:63	iridescent CNC/Nano-lignin films	32:63	iridescent CNC/Nano-lignin films	32:63	Nature-inspired construction of iridescent CNC/Nano-lignin films for UV resistance and ultra-fast humidity response.					
36087975	5	39	from	capabilities	786:797	arg1	region					806:811	UVB region	802:811	UVB region	802:811	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	5	39	from	capabilities	786:797	arg1	hydrophobicity					827:840	increased hydrophobicity	817:840	increased hydrophobicity	817:840	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	3	40	theme	iridescent	487:496	arg1	films					498:502	the nanocomposites iridescent films	468:502	the nanocomposites iridescent films with enlarged helix pitches	468:530	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	0	41	theme	UV	69:70	arg1	resistance					72:81	UV resistance	69:81	UV resistance	69:81	Nature-inspired construction of iridescent CNC/Nano-lignin films for UV resistance and ultra-fast humidity response.					
36087975	5	42	theme	lignin	945:950	arg1	presence					921:928	the presence	917:928	the presence of hydrophobic lignin	917:950	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	5	43	theme	absorption	737:746	arg1	capabilities					748:759	Distinguished UV absorption capabilities	720:759	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75°	720:870	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	2	44	theme	photonic	252:259	arg1	films					261:265	nature-inspired photonic films	236:265	nature-inspired photonic films	236:265	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	1	45	theme	functional	132:141	arg1	materials					143:151	functional materials	132:151	functional materials based on sustainable and renewable biomolecules	132:199	Exploration of functional materials based on sustainable and renewable biomolecules has been of much interest.					
36087975	4	46	theme	homogeneous	643:653	arg1	distribution					655:666	homogeneous distribution	643:666	homogeneous distribution	643:666	Decreased crystallite sizes and expanding layer gaps indicated the homogeneous distribution and hydrophobic interactions between CNCs and LNPs.					
36087975	4	47	theme	Decreased	576:584	arg1	sizes					598:602	Decreased crystallite sizes	576:602	Decreased crystallite sizes	576:602	Decreased crystallite sizes and expanding layer gaps indicated the homogeneous distribution and hydrophobic interactions between CNCs and LNPs.					
36087975	2	48	theme	nature-inspired	236:250	arg1	films					261:265	nature-inspired photonic films	236:265	nature-inspired photonic films	236:265	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	1	49	theme	materials	143:151	arg1	Exploration					117:127	Exploration	117:127	Exploration of functional materials based on sustainable and renewable biomolecules	117:199	Exploration of functional materials based on sustainable and renewable biomolecules has been of much interest.					
36087975	6	50	theme	outstanding	992:1002	arg1	cytocompatibility					1004:1020	outstanding cytocompatibility	992:1020	outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices	992:1199	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	6	51	theme	great	1103:1107	arg1	potentials					1109:1118	great potentials	1103:1118	great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices	1103:1199	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	2	52	theme	cellulose	359:367	arg1	crystals					369:376	chiral nematic cellulose crystals	344:376	chiral nematic cellulose crystals (CNCs)	344:383	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	2	52	theme	cellulose	359:367	arg1	CNCs					379:382	CNCs	379:382	CNCs	379:382	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	3	53	theme	LNPs	570:573	arg1	LNPs					570:573	LNPs	570:573	LNPs	570:573	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	3	53	theme	LNPs	570:573	arg1	amounts					559:565	higher amounts	552:565	higher amounts of LNPs	552:573	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	4	54	dep	distribution	655:666	arg1	the					639:641	the	639:641	the	639:641	Decreased crystallite sizes and expanding layer gaps indicated the homogeneous distribution and hydrophobic interactions between CNCs and LNPs.					
36087975	2	55	theme	nematic	351:357	arg1	crystals					369:376	chiral nematic cellulose crystals	344:376	chiral nematic cellulose crystals (CNCs)	344:383	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	2	55	theme	nematic	351:357	arg1	CNCs					379:382	CNCs	379:382	CNCs	379:382	Herein, nature-inspired photonic films were proposed by incorporation of bio-based lignin nanoparticles (LNPs) into chiral nematic cellulose crystals (CNCs).					
36087975	3	56	theme	enlarged	509:516	arg1	pitches					524:530	enlarged helix pitches	509:530	enlarged helix pitches	509:530	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36087975	5	57	theme	increased	817:825	arg1	hydrophobicity					827:840	increased hydrophobicity	817:840	increased hydrophobicity	817:840	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	6	58	theme	wearable	1184:1191	arg1	devices					1193:1199	wearable devices	1184:1199	wearable devices	1184:1199	The proposed optical films also showed outstanding cytocompatibility owing to all-natural components introduced into the materials, which may display great potentials in many fields such as stimuli sensing, anti-counterfeiting and wearable devices.					
36087975	5	59	theme	composite	894:902	arg1	films					904:908	the composite films	890:908	the composite films due to the presence of hydrophobic lignin	890:950	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	1	60	theme	sustainable	162:172	arg1	biomolecules					188:199	sustainable and renewable biomolecules	162:199	sustainable and renewable biomolecules	162:199	Exploration of functional materials based on sustainable and renewable biomolecules has been of much interest.					
36087975	4	61	theme	hydrophobic	672:682	arg1	interactions					684:695	hydrophobic interactions	672:695	hydrophobic interactions	672:695	Decreased crystallite sizes and expanding layer gaps indicated the homogeneous distribution and hydrophobic interactions between CNCs and LNPs.					
36087975	5	62	dep	capabilities	786:797	arg1	%					774:774	%	774:774	%	774:774	Distinguished UV absorption capabilities with over 90 % shielding capabilities in UVB region and increased hydrophobicity with the contact angle of 75° were achieved for the composite films due to the presence of hydrophobic lignin.					
36087975	3	63	theme	amounts	559:565	arg1	intercalation					535:547	intercalation	535:547	intercalation of higher amounts of LNPs	535:573	Evaporation induced self-assembly (EISA) formed oriented and layered structure of the nanocomposites iridescent films with enlarged helix pitches by intercalation of higher amounts of LNPs.					
36796564	15	0	dep	Red	1944:1946	arg1	141					1948:1950	141	1948:1950	141	1948:1950	The maximum adsorption capacity of SnO-IG was 540.5, 584.7, 1501.5, and 1100.1 mg/g for Red 141, Red 195, Red 198, and Yellow 18 dyes, respectively.					
36796564	11	1	theme	FT-IR	1404:1408	arg1	techniques					1444:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	These hybrid material structures were characterized by FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques.					
36796564	2	2	theme	environmental	301:313	arg1	studies					315:321	environmental studies	301:321	environmental studies	301:321	These composites have some applications in solid-state, energy storage devices and environmental studies.					
36796564	2	3	from	applications	245:256	arg1	devices					289:295	energy storage devices	274:295	energy storage devices	274:295	These composites have some applications in solid-state, energy storage devices and environmental studies.					
36796564	2	3	from	applications	245:256	arg1	studies					315:321	environmental studies	301:321	environmental studies	301:321	These composites have some applications in solid-state, energy storage devices and environmental studies.					
36796564	2	3	from	applications	245:256	arg1	solid-state					261:271	solid-state	261:271	solid-state	261:271	These composites have some applications in solid-state, energy storage devices and environmental studies.					
36796564	8	4	theme	electrostatic	993:1005	arg1	interactions					1028:1039	electrostatic and hydrogen bonding interactions	993:1039	interactions	1028:1039	The microstructure of the ionogel was involved as assembled unit via π-π, electrostatic and hydrogen bonding interactions to be three-dimensional networks.					
36796564	16	5	theme	prepared	2076:2083	arg1	biocomposite					2092:2103	the prepared SnO-IG biocomposite	2072:2103	the prepared SnO-IG biocomposite for dye removal from textile wastewater	2072:2143	Also, an acceptable result (96.47 % dye removal) was obtained with the prepared SnO-IG biocomposite for dye removal from textile wastewater.					
36796564	10	6	theme	nanostructure	1258:1270	arg1	chitosan					1200:1207	chitosan	1200:1207	chitosan	1200:1207	When chitosan was contained as the interlayer space of the SnO nanostructure, the resulting biocomposite formed a well-ordered flower-like SnO structure.					
36796564	10	6	theme	nanostructure	1258:1270	arg1	space					1241:1245	the interlayer space	1226:1245	the interlayer space of the SnO nanostructure	1226:1270	When chitosan was contained as the interlayer space of the SnO nanostructure, the resulting biocomposite formed a well-ordered flower-like SnO structure.					
36796564	3	7	theme	SnO	491:493	arg1	nanoplate					495:503	a SnO nanoplate	489:503	a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG)	489:531	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	14	8	theme	removal	1663:1669	arg1	%					1723:1723	98.5, 98.8, 97.9, and 98.4 %	1696:1723	98.5, 98.8, 97.9, and 98.4 %	1696:1723	The dye removal efficiency of SnO-IG was 98.5, 98.8, 97.9, and 98.4 % via the second-order kinetic model for Reactive Red 141, Reactive Red 195, Reactive Red 198, and Reactive Yellow 18, respectively.					
36796564	14	8	theme	removal	1663:1669	arg1	efficiency					1671:1680	The dye removal efficiency	1655:1680	The dye removal efficiency of SnO-IG	1655:1690	The dye removal efficiency of SnO-IG was 98.5, 98.8, 97.9, and 98.4 % via the second-order kinetic model for Reactive Red 141, Reactive Red 195, Reactive Red 198, and Reactive Yellow 18, respectively.					
36796564	8	9	theme	hydrogen	1011:1018	arg1	interactions					1028:1039	electrostatic and hydrogen bonding interactions	993:1039	interactions	1028:1039	The microstructure of the ionogel was involved as assembled unit via π-π, electrostatic and hydrogen bonding interactions to be three-dimensional networks.					
36796564	12	10	from	changes	1460:1466	arg1	values					1484:1489	the band gap values	1471:1489	the band gap values for photocatalysis applications	1471:1521	The changes in the band gap values for photocatalysis applications were investigated.					
36796564	4	11	theme	pyridine	599:606	arg1	refluxed					646:653	refluxed	646:653	refluxed	646:653	For the preparation of the ethyl pyridinium iodide, a mixture of pyridine and iodoethane (1: 2 molar ratio) was refluxed for 24 h.					
36796564	4	11	theme	pyridine	599:606	arg1	mixture					588:594	a mixture	586:594	a mixture of pyridine and iodoethane (1: 2 molar ratio)	586:640	For the preparation of the ethyl pyridinium iodide, a mixture of pyridine and iodoethane (1: 2 molar ratio) was refluxed for 24 h.					
36796564	13	12	theme	SnO-IL	1562:1567	arg1	case					1549:1552	the case	1545:1552	the case of SnO, SnO-IL, SnO-CS, and SnO-IG	1545:1587	In the case of SnO, SnO-IL, SnO-CS, and SnO-IG, the band gap energy was 3.9, 3.6, 3.2, and 2.8 eV, respectively.					
36796564	11	13	theme	XRD	1411:1413	arg1	techniques					1444:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	These hybrid material structures were characterized by FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques.					
36796564	1	14	theme	polymeric	199:207	arg1	network					209:215	polymeric network	199:215	polymeric network	199:215	Ionogels are a category of hybrid material containing ionic liquid stabilized by polymeric network.					
36796564	6	15	theme	ionogel	825:831	arg1	pH					815:816	the pH	811:816	the pH of the ionogel	811:831	By increasing NH3·H2O, the pH of the ionogel reached 7-8.					
36796564	4	16	theme	pyridinium	567:576	arg1	iodide					578:583	the ethyl pyridinium iodide	557:583	the ethyl pyridinium iodide	557:583	For the preparation of the ethyl pyridinium iodide, a mixture of pyridine and iodoethane (1: 2 molar ratio) was refluxed for 24 h.					
36796564	13	17	theme	SnO-CS	1570:1575	arg1	case					1549:1552	the case	1545:1552	the case of SnO, SnO-IL, SnO-CS, and SnO-IG	1545:1587	In the case of SnO, SnO-IL, SnO-CS, and SnO-IG, the band gap energy was 3.9, 3.6, 3.2, and 2.8 eV, respectively.					
36796564	7	18	theme	ultrasonic	894:903	arg1	bath					905:908	an ultrasonic bath	891:908	an ultrasonic bath for 1 h	891:916	Then, the resultant IG was mixed with SnO in an ultrasonic bath for 1 h.					
36796564	9	19	theme	gap	1183:1185	arg1	values					1187:1192	band gap values	1178:1192	band gap values	1178:1192	The intercalated ionic liquid and chitosan influenced the stability of the SnO nanoplates and improved band gap values.					
36796564	13	20	theme	SnO	1557:1559	arg1	case					1549:1552	the case	1545:1552	the case of SnO, SnO-IL, SnO-CS, and SnO-IG	1545:1587	In the case of SnO, SnO-IL, SnO-CS, and SnO-IG, the band gap energy was 3.9, 3.6, 3.2, and 2.8 eV, respectively.					
36796564	7	21	theme	resultant	856:864	arg1	IG					866:867	the resultant IG	852:867	the resultant IG	852:867	Then, the resultant IG was mixed with SnO in an ultrasonic bath for 1 h.					
36796564	2	22	theme	energy	274:279	arg1	devices					289:295	energy storage devices	274:295	energy storage devices	274:295	These composites have some applications in solid-state, energy storage devices and environmental studies.					
36796564	4	23	theme	iodoethane	612:621	arg1	refluxed					646:653	refluxed	646:653	refluxed	646:653	For the preparation of the ethyl pyridinium iodide, a mixture of pyridine and iodoethane (1: 2 molar ratio) was refluxed for 24 h.					
36796564	4	23	theme	iodoethane	612:621	arg1	mixture					588:594	a mixture	586:594	a mixture of pyridine and iodoethane (1: 2 molar ratio)	586:640	For the preparation of the ethyl pyridinium iodide, a mixture of pyridine and iodoethane (1: 2 molar ratio) was refluxed for 24 h.					
36796564	0	24	theme	Band	61:64	arg1	process					109:115	Band gap narrow and adsorption-photodegradation process	61:115	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.	0:116	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.					
36796564	5	25	theme	liquid	724:729	arg1	ionic					718:722	ethyl pyridinium iodide ionic	694:722	ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v)	694:785	The ionogel was formed using ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v).					
36796564	1	26	theme	material	152:159	arg1	category					133:140	a category	131:140	a category of hybrid material containing ionic liquid stabilized by polymeric network	131:215	Ionogels are a category of hybrid material containing ionic liquid stabilized by polymeric network.					
36796564	1	26	theme	material	152:159	arg1	Ionogels					118:125	Ionogels	118:125	Ionogels	118:125	Ionogels are a category of hybrid material containing ionic liquid stabilized by polymeric network.					
36796564	14	27	theme	Reactive	1822:1829	arg1	Yellow					1831:1836	Reactive Yellow 18	1822:1839	Reactive Yellow 18	1822:1839	The dye removal efficiency of SnO-IG was 98.5, 98.8, 97.9, and 98.4 % via the second-order kinetic model for Reactive Red 141, Reactive Red 195, Reactive Red 198, and Reactive Yellow 18, respectively.					
36796564	0	28	theme	narrow	70:75	arg1	process					109:115	Band gap narrow and adsorption-photodegradation process	61:115	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.	0:116	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.					
36796564	3	29	theme	ethyl	357:361	arg1	IL					395:396	ethyl pyridinium iodide ionic liquid (IL)	357:397	ethyl pyridinium iodide ionic liquid (IL)	357:397	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	11	30	theme	material	1362:1369	arg1	structures					1371:1380	These hybrid material structures	1349:1380	These hybrid material structures	1349:1380	These hybrid material structures were characterized by FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques.					
36796564	0	31	theme	adsorption-photodegradation	81:107	arg1	process					109:115	Band gap narrow and adsorption-photodegradation process	61:115	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.	0:116	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.					
36796564	9	32	theme	SnO	1150:1152	arg1	nanoplates					1154:1163	the SnO nanoplates	1146:1163	the SnO nanoplates	1146:1163	The intercalated ionic liquid and chitosan influenced the stability of the SnO nanoplates and improved band gap values.					
36796564	14	33	theme	second-order	1733:1744	arg1	model					1754:1758	the second-order kinetic model	1729:1758	the second-order kinetic model for Reactive Red 141, Reactive Red 195, Reactive Red 198, and Reactive Yellow 18, respectively	1729:1853	The dye removal efficiency of SnO-IG was 98.5, 98.8, 97.9, and 98.4 % via the second-order kinetic model for Reactive Red 141, Reactive Red 195, Reactive Red 198, and Reactive Yellow 18, respectively.					
36796564	10	34	theme	flower-like	1322:1332	arg1	structure					1338:1346	a well-ordered flower-like SnO structure	1307:1346	a well-ordered flower-like SnO structure	1307:1346	When chitosan was contained as the interlayer space of the SnO nanostructure, the resulting biocomposite formed a well-ordered flower-like SnO structure.					
36796564	16	35	from	wastewater	2134:2143	arg1	removal					2113:2119	dye removal	2109:2119	dye removal from textile wastewater	2109:2143	Also, an acceptable result (96.47 % dye removal) was obtained with the prepared SnO-IG biocomposite for dye removal from textile wastewater.					
36796564	12	36	theme	gap	1480:1482	arg1	values					1484:1489	the band gap values	1471:1489	the band gap values for photocatalysis applications	1471:1521	The changes in the band gap values for photocatalysis applications were investigated.					
36796564	3	37	theme	liquid	387:392	arg1	IL					395:396	ethyl pyridinium iodide ionic liquid (IL)	357:397	ethyl pyridinium iodide ionic liquid (IL)	357:397	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	0	38	theme	Intercalated	0:11	arg1	ionogel					35:41	Intercalated chitosan-ionic liquid ionogel	0:41	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.	0:116	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.					
36796564	9	39	theme	band	1178:1181	arg1	values					1187:1192	band gap values	1178:1192	band gap values	1178:1192	The intercalated ionic liquid and chitosan influenced the stability of the SnO nanoplates and improved band gap values.					
36796564	3	40	theme	iodide	374:379	arg1	IL					395:396	ethyl pyridinium iodide ionic liquid (IL)	357:397	ethyl pyridinium iodide ionic liquid (IL)	357:397	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	0	41	theme	liquid	28:33	arg1	ionogel					35:41	Intercalated chitosan-ionic liquid ionogel	0:41	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.	0:116	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.					
36796564	15	42	theme	SnO-IG	1891:1896	arg1	capacity					1879:1886	The maximum adsorption capacity	1856:1886	The maximum adsorption capacity of SnO-IG	1856:1896	The maximum adsorption capacity of SnO-IG was 540.5, 584.7, 1501.5, and 1100.1 mg/g for Red 141, Red 195, Red 198, and Yellow 18 dyes, respectively.					
36796564	15	42	theme	SnO-IG	1891:1896	arg1	540.5					1902:1906	540.5	1902:1906	540.5	1902:1906	The maximum adsorption capacity of SnO-IG was 540.5, 584.7, 1501.5, and 1100.1 mg/g for Red 141, Red 195, Red 198, and Yellow 18 dyes, respectively.					
36796564	16	43	theme	textile	2126:2132	arg1	wastewater					2134:2143	textile wastewater	2126:2143	textile wastewater	2126:2143	Also, an acceptable result (96.47 % dye removal) was obtained with the prepared SnO-IG biocomposite for dye removal from textile wastewater.					
36796564	3	44	used	used	462:465	arg2	ionogel					404:410	ionogel	404:410	ionogel	404:410	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	3	44	used	used	462:465	arg2	CS					352:353	CS	352:353	CS	352:353	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	3	44	used	used	462:465	arg2	IL					395:396	ethyl pyridinium iodide ionic liquid (IL)	357:397	ethyl pyridinium iodide ionic liquid (IL)	357:397	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	3	44	used	used	462:465	arg2	chitosan					342:349	chitosan	342:349	chitosan (CS)	342:354	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	3	44	used	used	462:465	arg2	IG					413:414	IG	413:414	IG	413:414	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	16	45	theme	acceptable	2014:2023	arg1	removal					2045:2051	96.47 % dye removal	2033:2051	96.47 % dye removal	2033:2051	Also, an acceptable result (96.47 % dye removal) was obtained with the prepared SnO-IG biocomposite for dye removal from textile wastewater.					
36796564	16	45	theme	acceptable	2014:2023	arg1	result					2025:2030	an acceptable result	2011:2030	an acceptable result (96.47 % dye removal)	2011:2052	Also, an acceptable result (96.47 % dye removal) was obtained with the prepared SnO-IG biocomposite for dye removal from textile wastewater.					
36796564	5	46	theme	ethyl	694:698	arg1	ionic					718:722	ethyl pyridinium iodide ionic	694:722	ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v)	694:785	The ionogel was formed using ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v).					
36796564	15	47	dep	Red	1953:1955	arg1	195					1957:1959	195	1957:1959	195	1957:1959	The maximum adsorption capacity of SnO-IG was 540.5, 584.7, 1501.5, and 1100.1 mg/g for Red 141, Red 195, Red 198, and Yellow 18 dyes, respectively.					
36796564	13	48	from	3.9	1614:1616	arg1	case					1549:1552	the case	1545:1552	the case of SnO, SnO-IL, SnO-CS, and SnO-IG	1545:1587	In the case of SnO, SnO-IL, SnO-CS, and SnO-IG, the band gap energy was 3.9, 3.6, 3.2, and 2.8 eV, respectively.					
36796564	16	49	theme	%	2039:2039	arg1	removal					2045:2051	96.47 % dye removal	2033:2051	96.47 % dye removal	2033:2051	Also, an acceptable result (96.47 % dye removal) was obtained with the prepared SnO-IG biocomposite for dye removal from textile wastewater.					
36796564	16	49	theme	%	2039:2039	arg1	result					2025:2030	an acceptable result	2011:2030	an acceptable result (96.47 % dye removal)	2011:2052	Also, an acceptable result (96.47 % dye removal) was obtained with the prepared SnO-IG biocomposite for dye removal from textile wastewater.					
36796564	11	50	theme	SEM	1416:1418	arg1	techniques					1444:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	These hybrid material structures were characterized by FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques.					
36796564	2	51	contain	have	235:238	arg2	applications					245:256	some applications	240:256	some applications in solid-state, energy storage devices and environmental studies	240:321	These composites have some applications in solid-state, energy storage devices and environmental studies.					
36796564	2	51	contain	have	235:238	arg1	composites					224:233	These composites	218:233	These composites	218:233	These composites have some applications in solid-state, energy storage devices and environmental studies.					
36796564	8	52	theme	ionogel	945:951	arg1	unit					979:982	assembled unit	969:982	assembled unit	969:982	The microstructure of the ionogel was involved as assembled unit via π-π, electrostatic and hydrogen bonding interactions to be three-dimensional networks.					
36796564	8	52	theme	ionogel	945:951	arg1	microstructure					923:936	The microstructure	919:936	The microstructure of the ionogel	919:951	The microstructure of the ionogel was involved as assembled unit via π-π, electrostatic and hydrogen bonding interactions to be three-dimensional networks.					
36796564	11	53	theme	DRS	1440:1442	arg1	techniques					1444:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	These hybrid material structures were characterized by FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques.					
36796564	15	54	theme	adsorption	1868:1877	arg1	capacity					1879:1886	The maximum adsorption capacity	1856:1886	The maximum adsorption capacity of SnO-IG	1856:1896	The maximum adsorption capacity of SnO-IG was 540.5, 584.7, 1501.5, and 1100.1 mg/g for Red 141, Red 195, Red 198, and Yellow 18 dyes, respectively.					
36796564	15	54	theme	adsorption	1868:1877	arg1	540.5					1902:1906	540.5	1902:1906	540.5	1902:1906	The maximum adsorption capacity of SnO-IG was 540.5, 584.7, 1501.5, and 1100.1 mg/g for Red 141, Red 195, Red 198, and Yellow 18 dyes, respectively.					
36796564	13	55	theme	gap	1599:1601	arg1	3.9					1614:1616	3.9	1614:1616	3.9	1614:1616	In the case of SnO, SnO-IL, SnO-CS, and SnO-IG, the band gap energy was 3.9, 3.6, 3.2, and 2.8 eV, respectively.					
36796564	13	55	theme	gap	1599:1601	arg1	energy					1603:1608	the band gap energy	1590:1608	the band gap energy	1590:1608	In the case of SnO, SnO-IL, SnO-CS, and SnO-IG, the band gap energy was 3.9, 3.6, 3.2, and 2.8 eV, respectively.					
36796564	8	56	theme	assembled	969:977	arg1	unit					979:982	assembled unit	969:982	assembled unit	969:982	The microstructure of the ionogel was involved as assembled unit via π-π, electrostatic and hydrogen bonding interactions to be three-dimensional networks.					
36796564	8	56	theme	assembled	969:977	arg1	microstructure					923:936	The microstructure	919:936	The microstructure of the ionogel	919:951	The microstructure of the ionogel was involved as assembled unit via π-π, electrostatic and hydrogen bonding interactions to be three-dimensional networks.					
36796564	10	57	theme	interlayer	1230:1239	arg1	chitosan					1200:1207	chitosan	1200:1207	chitosan	1200:1207	When chitosan was contained as the interlayer space of the SnO nanostructure, the resulting biocomposite formed a well-ordered flower-like SnO structure.					
36796564	10	57	theme	interlayer	1230:1239	arg1	space					1241:1245	the interlayer space	1226:1245	the interlayer space of the SnO nanostructure	1226:1270	When chitosan was contained as the interlayer space of the SnO nanostructure, the resulting biocomposite formed a well-ordered flower-like SnO structure.					
36796564	3	58	theme	nanoplate	495:503	arg1	preparation					474:484	the preparation	470:484	the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG)	470:531	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	16	59	theme	SnO-IG	2085:2090	arg1	biocomposite					2092:2103	the prepared SnO-IG biocomposite	2072:2103	the prepared SnO-IG biocomposite for dye removal from textile wastewater	2072:2143	Also, an acceptable result (96.47 % dye removal) was obtained with the prepared SnO-IG biocomposite for dye removal from textile wastewater.					
36796564	13	60	theme	SnO-IG	1582:1587	arg1	case					1549:1552	the case	1545:1552	the case of SnO, SnO-IL, SnO-CS, and SnO-IG	1545:1587	In the case of SnO, SnO-IL, SnO-CS, and SnO-IG, the band gap energy was 3.9, 3.6, 3.2, and 2.8 eV, respectively.					
36796564	1	61	contain	containing	161:170	arg1	material					152:159	hybrid material	145:159	hybrid material containing ionic liquid stabilized by polymeric network	145:215	Ionogels are a category of hybrid material containing ionic liquid stabilized by polymeric network.					
36796564	1	61	contain	containing	161:170	arg2	liquid					178:183	liquid	178:183	liquid	178:183	Ionogels are a category of hybrid material containing ionic liquid stabilized by polymeric network.					
36796564	14	62	theme	dye	1659:1661	arg1	%					1723:1723	98.5, 98.8, 97.9, and 98.4 %	1696:1723	98.5, 98.8, 97.9, and 98.4 %	1696:1723	The dye removal efficiency of SnO-IG was 98.5, 98.8, 97.9, and 98.4 % via the second-order kinetic model for Reactive Red 141, Reactive Red 195, Reactive Red 198, and Reactive Yellow 18, respectively.					
36796564	14	62	theme	dye	1659:1661	arg1	efficiency					1671:1680	The dye removal efficiency	1655:1680	The dye removal efficiency of SnO-IG	1655:1690	The dye removal efficiency of SnO-IG was 98.5, 98.8, 97.9, and 98.4 % via the second-order kinetic model for Reactive Red 141, Reactive Red 195, Reactive Red 198, and Reactive Yellow 18, respectively.					
36796564	15	63	theme	maximum	1860:1866	arg1	capacity					1879:1886	The maximum adsorption capacity	1856:1886	The maximum adsorption capacity of SnO-IG	1856:1896	The maximum adsorption capacity of SnO-IG was 540.5, 584.7, 1501.5, and 1100.1 mg/g for Red 141, Red 195, Red 198, and Yellow 18 dyes, respectively.					
36796564	15	63	theme	maximum	1860:1866	arg1	540.5					1902:1906	540.5	1902:1906	540.5	1902:1906	The maximum adsorption capacity of SnO-IG was 540.5, 584.7, 1501.5, and 1100.1 mg/g for Red 141, Red 195, Red 198, and Yellow 18 dyes, respectively.					
36796564	10	64	theme	SnO	1254:1256	arg1	nanostructure					1258:1270	the SnO nanostructure	1250:1270	the SnO nanostructure	1250:1270	When chitosan was contained as the interlayer space of the SnO nanostructure, the resulting biocomposite formed a well-ordered flower-like SnO structure.					
36796564	5	65	theme	acetic	765:770	arg1	v/v					782:784	1 % v/v	778:784	1 % v/v	778:784	The ionogel was formed using ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v).					
36796564	5	65	theme	acetic	765:770	arg1	acid					772:775	acetic acid	765:775	acetic acid (1 % v/v)	765:785	The ionogel was formed using ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v).					
36796564	8	66	theme	bonding	1020:1026	arg1	interactions					1028:1039	electrostatic and hydrogen bonding interactions	993:1039	interactions	1028:1039	The microstructure of the ionogel was involved as assembled unit via π-π, electrostatic and hydrogen bonding interactions to be three-dimensional networks.					
36796564	16	67	theme	96.47 	2033:2038	arg1	%					2039:2039	%	2039:2039	%	2039:2039	Also, an acceptable result (96.47 % dye removal) was obtained with the prepared SnO-IG biocomposite for dye removal from textile wastewater.					
36796564	4	68	theme	ethyl	561:565	arg1	iodide					578:583	the ethyl pyridinium iodide	557:583	the ethyl pyridinium iodide	557:583	For the preparation of the ethyl pyridinium iodide, a mixture of pyridine and iodoethane (1: 2 molar ratio) was refluxed for 24 h.					
36796564	4	69	theme	iodide	578:583	arg1	preparation					542:552	the preparation	538:552	the preparation of the ethyl pyridinium iodide	538:583	For the preparation of the ethyl pyridinium iodide, a mixture of pyridine and iodoethane (1: 2 molar ratio) was refluxed for 24 h.					
36796564	8	70	theme	three-dimensional	1047:1063	arg1	networks					1065:1072	three-dimensional networks	1047:1072	three-dimensional networks	1047:1072	The microstructure of the ionogel was involved as assembled unit via π-π, electrostatic and hydrogen bonding interactions to be three-dimensional networks.					
36796564	2	71	theme	storage	281:287	arg1	devices					289:295	energy storage devices	274:295	energy storage devices	274:295	These composites have some applications in solid-state, energy storage devices and environmental studies.					
36796564	5	72	from	chitosan	734:741	arg1	liquid					724:729	liquid	724:729	liquid	724:729	The ionogel was formed using ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v).					
36796564	3	73	dep	nanoplate	495:503	arg1	SnO-IG					525:530	SnO-IG	525:530	SnO-IG	525:530	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	3	73	dep	nanoplate	495:503	arg1	SnO-CS					514:519	SnO-CS	514:519	SnO-CS	514:519	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	3	73	dep	nanoplate	495:503	arg1	SnO-IL					506:511	SnO-IL	506:511	SnO-IL	506:511	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	4	74	theme	molar	629:633	arg1	pyridine					599:606	pyridine	599:606	pyridine	599:606	For the preparation of the ethyl pyridinium iodide, a mixture of pyridine and iodoethane (1: 2 molar ratio) was refluxed for 24 h.					
36796564	4	74	theme	molar	629:633	arg1	ratio					635:639	1: 2 molar ratio	624:639	1: 2 molar ratio	624:639	For the preparation of the ethyl pyridinium iodide, a mixture of pyridine and iodoethane (1: 2 molar ratio) was refluxed for 24 h.					
36796564	0	75	theme	gap	66:68	arg1	process					109:115	Band gap narrow and adsorption-photodegradation process	61:115	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.	0:116	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.					
36796564	1	76	theme	hybrid	145:150	arg1	material					152:159	hybrid material	145:159	hybrid material containing ionic liquid stabilized by polymeric network	145:215	Ionogels are a category of hybrid material containing ionic liquid stabilized by polymeric network.					
36796564	0	77	from	ionogel	35:41	arg1	nanoplate					50:58	SnO nanoplate	46:58	SnO nanoplate	46:58	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.					
36796564	5	78	theme	%	780:780	arg1	v/v					782:784	1 % v/v	778:784	1 % v/v	778:784	The ionogel was formed using ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v).					
36796564	5	78	theme	%	780:780	arg1	acid					772:775	acetic acid	765:775	acetic acid (1 % v/v)	765:785	The ionogel was formed using ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v).					
36796564	10	79	theme	resulting	1277:1285	arg1	biocomposite					1287:1298	the resulting biocomposite	1273:1298	the resulting biocomposite	1273:1298	When chitosan was contained as the interlayer space of the SnO nanostructure, the resulting biocomposite formed a well-ordered flower-like SnO structure.					
36796564	11	80	theme	hybrid	1355:1360	arg1	structures					1371:1380	These hybrid material structures	1349:1380	These hybrid material structures	1349:1380	These hybrid material structures were characterized by FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques.					
36796564	14	81	theme	kinetic	1746:1752	arg1	model					1754:1758	the second-order kinetic model	1729:1758	the second-order kinetic model for Reactive Red 141, Reactive Red 195, Reactive Red 198, and Reactive Yellow 18, respectively	1729:1853	The dye removal efficiency of SnO-IG was 98.5, 98.8, 97.9, and 98.4 % via the second-order kinetic model for Reactive Red 141, Reactive Red 195, Reactive Red 198, and Reactive Yellow 18, respectively.					
36796564	12	82	theme	photocatalysis	1495:1508	arg1	applications					1510:1521	photocatalysis applications	1495:1521	photocatalysis applications	1495:1521	The changes in the band gap values for photocatalysis applications were investigated.					
36796564	3	83	theme	pyridinium	363:372	arg1	IL					395:396	ethyl pyridinium iodide ionic liquid (IL)	357:397	ethyl pyridinium iodide ionic liquid (IL)	357:397	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	9	84	theme	nanoplates	1154:1163	arg1	stability					1133:1141	the stability	1129:1141	the stability of the SnO nanoplates	1129:1163	The intercalated ionic liquid and chitosan influenced the stability of the SnO nanoplates and improved band gap values.					
36796564	10	85	theme	well-ordered	1309:1320	arg1	structure					1338:1346	a well-ordered flower-like SnO structure	1307:1346	a well-ordered flower-like SnO structure	1307:1346	When chitosan was contained as the interlayer space of the SnO nanostructure, the resulting biocomposite formed a well-ordered flower-like SnO structure.					
36796564	5	86	theme	pyridinium	700:709	arg1	ionic					718:722	ethyl pyridinium iodide ionic	694:722	ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v)	694:785	The ionogel was formed using ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v).					
36796564	12	87	theme	band	1475:1478	arg1	values					1484:1489	the band gap values	1471:1489	the band gap values for photocatalysis applications	1471:1521	The changes in the band gap values for photocatalysis applications were investigated.					
36796564	5	88	theme	iodide	711:716	arg1	ionic					718:722	ethyl pyridinium iodide ionic	694:722	ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v)	694:785	The ionogel was formed using ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v).					
36796564	3	89	theme	ionic	381:385	arg1	IL					395:396	ethyl pyridinium iodide ionic liquid (IL)	357:397	ethyl pyridinium iodide ionic liquid (IL)	357:397	In this research, chitosan (CS), ethyl pyridinium iodide ionic liquid (IL), and ionogel (IG) consisting of chitosan and ionic liquid were used in the preparation of a SnO nanoplate (SnO-IL, SnO-CS and SnO-IG).					
36796564	0	90	theme	SnO	46:48	arg1	nanoplate					50:58	SnO nanoplate	46:58	SnO nanoplate	46:58	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.					
36796564	5	91	from	liquid	724:729	arg1	chitosan					734:741	chitosan	734:741	chitosan that was dissolved in acetic acid (1 % v/v)	734:785	The ionogel was formed using ethyl pyridinium iodide ionic liquid in chitosan that was dissolved in acetic acid (1 % v/v).					
36796564	16	92	theme	dye	2109:2111	arg1	removal					2113:2119	dye removal	2109:2119	dye removal from textile wastewater	2109:2143	Also, an acceptable result (96.47 % dye removal) was obtained with the prepared SnO-IG biocomposite for dye removal from textile wastewater.					
36796564	10	93	theme	SnO	1334:1336	arg1	structure					1338:1346	a well-ordered flower-like SnO structure	1307:1346	a well-ordered flower-like SnO structure	1307:1346	When chitosan was contained as the interlayer space of the SnO nanostructure, the resulting biocomposite formed a well-ordered flower-like SnO structure.					
36796564	11	94	theme	DSC	1426:1428	arg1	techniques					1444:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	These hybrid material structures were characterized by FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques.					
36796564	11	95	theme	TGA	1421:1423	arg1	techniques					1444:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	These hybrid material structures were characterized by FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques.					
36796564	0	96	dep	ionogel	35:41	arg1	process					109:115	Band gap narrow and adsorption-photodegradation process	61:115	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.	0:116	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.					
36796564	16	97	theme	dye	2041:2043	arg1	removal					2045:2051	96.47 % dye removal	2033:2051	96.47 % dye removal	2033:2051	Also, an acceptable result (96.47 % dye removal) was obtained with the prepared SnO-IG biocomposite for dye removal from textile wastewater.					
36796564	16	97	theme	dye	2041:2043	arg1	result					2025:2030	an acceptable result	2011:2030	an acceptable result (96.47 % dye removal)	2011:2052	Also, an acceptable result (96.47 % dye removal) was obtained with the prepared SnO-IG biocomposite for dye removal from textile wastewater.					
36796564	15	98	theme	Yellow	1975:1980	arg1	dyes					1985:1988	Yellow 18 dyes	1975:1988	Yellow 18 dyes	1975:1988	The maximum adsorption capacity of SnO-IG was 540.5, 584.7, 1501.5, and 1100.1 mg/g for Red 141, Red 195, Red 198, and Yellow 18 dyes, respectively.					
36796564	15	99	dep	Red	1962:1964	arg1	198					1966:1968	198	1966:1968	198	1966:1968	The maximum adsorption capacity of SnO-IG was 540.5, 584.7, 1501.5, and 1100.1 mg/g for Red 141, Red 195, Red 198, and Yellow 18 dyes, respectively.					
36796564	14	100	theme	SnO-IG	1685:1690	arg1	%					1723:1723	98.5, 98.8, 97.9, and 98.4 %	1696:1723	98.5, 98.8, 97.9, and 98.4 %	1696:1723	The dye removal efficiency of SnO-IG was 98.5, 98.8, 97.9, and 98.4 % via the second-order kinetic model for Reactive Red 141, Reactive Red 195, Reactive Red 198, and Reactive Yellow 18, respectively.					
36796564	14	100	theme	SnO-IG	1685:1690	arg1	efficiency					1671:1680	The dye removal efficiency	1655:1680	The dye removal efficiency of SnO-IG	1655:1690	The dye removal efficiency of SnO-IG was 98.5, 98.8, 97.9, and 98.4 % via the second-order kinetic model for Reactive Red 141, Reactive Red 195, Reactive Red 198, and Reactive Yellow 18, respectively.					
36796564	13	101	theme	band	1594:1597	arg1	3.9					1614:1616	3.9	1614:1616	3.9	1614:1616	In the case of SnO, SnO-IL, SnO-CS, and SnO-IG, the band gap energy was 3.9, 3.6, 3.2, and 2.8 eV, respectively.					
36796564	13	101	theme	band	1594:1597	arg1	energy					1603:1608	the band gap energy	1590:1608	the band gap energy	1590:1608	In the case of SnO, SnO-IL, SnO-CS, and SnO-IG, the band gap energy was 3.9, 3.6, 3.2, and 2.8 eV, respectively.					
36796564	11	102	theme	BET	1431:1433	arg1	techniques					1444:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques	1404:1453	These hybrid material structures were characterized by FT-IR, XRD, SEM, TGA, DSC, BET, and DRS techniques.					
36796564	0	103	theme	chitosan-ionic	13:26	arg1	ionogel					35:41	Intercalated chitosan-ionic liquid ionogel	0:41	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.	0:116	Intercalated chitosan-ionic liquid ionogel in SnO nanoplate: Band gap narrow and adsorption-photodegradation process.					
36942860	2	0	theme	tumoroids	465:473	arg1	shape					436:440	shape	436:440	shape	436:440	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	2	0	theme	tumoroids	465:473	arg1	metabolism					447:456	metabolism	447:456	metabolism	447:456	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	2	0	theme	tumoroids	465:473	arg1	size					430:433	size	430:433	size	430:433	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	7	1	theme	available	1264:1272	arg1	space					1274:1278	available space	1264:1278	available space	1264:1278	Depending on available space, phenotypically different 3D cell assemblies have been observed inside the capsules, varying in the tightness of cell aggregations and their shapes.					
36942860	2	2	from	impact	416:421	arg1	shape					436:440	shape	436:440	shape	436:440	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	2	2	from	impact	416:421	arg1	metabolism					447:456	metabolism	447:456	metabolism	447:456	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	2	2	from	impact	416:421	arg1	size					430:433	size	430:433	size	430:433	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	7	3	theme	different	1296:1304	arg1	assemblies					1314:1323	phenotypically different 3D cell assemblies	1281:1323	phenotypically different 3D cell assemblies	1281:1323	Depending on available space, phenotypically different 3D cell assemblies have been observed inside the capsules, varying in the tightness of cell aggregations and their shapes.					
36942860	1	4	theme	in	272:273	arg1	models					287:292	numerous in vitro tumor models	263:292	numerous in vitro tumor models	263:292	Functional interaction between cancer cells and the surrounding microenvironment is still not sufficiently understood, which motivates the tremendous interest for the development of numerous in vitro tumor models.					
36942860	3	5	theme	geometries	687:696	arg1	spheroids					649:657	human hepatoma (HepG2) spheroids	626:657	human hepatoma (HepG2) spheroids of different dimensions and geometries	626:696	We demonstrate the fluidics-based low-cost methodology to reproducibly generate the alginate and alginate-chitosan microcapsules and apply it to grow human hepatoma (HepG2) spheroids of different dimensions and geometries.					
36942860	7	6	theme	3D	1306:1307	arg1	assemblies					1314:1323	phenotypically different 3D cell assemblies	1281:1323	phenotypically different 3D cell assemblies	1281:1323	Depending on available space, phenotypically different 3D cell assemblies have been observed inside the capsules, varying in the tightness of cell aggregations and their shapes.					
36942860	5	7	theme	mass	1019:1022	arg1	transfer					1024:1031	efficient mass transfer	1009:1031	efficient mass transfer	1009:1031	The diffusion of the selected benchmark molecules through the shell has been systematically investigated using both, experiments and simulations, which is essential to ensure efficient mass transfer and/or filtering of the biochemical species.					
36942860	6	8	theme	metabolic	1234:1242	arg1	assay					1244:1248	a metabolic assay	1232:1248	a metabolic assay	1232:1248	Metabolic activity of spheroids in microcapsules was confirmed by tracking the turnover of testosterone to androstenedione with chromatography studies in a metabolic assay.					
36942860	1	9	theme	tumor	281:285	arg1	models					287:292	numerous in vitro tumor models	263:292	numerous in vitro tumor models	263:292	Functional interaction between cancer cells and the surrounding microenvironment is still not sufficiently understood, which motivates the tremendous interest for the development of numerous in vitro tumor models.					
36942860	4	10	theme	shell	770:774	arg1	thickness					744:752	thickness	744:752	thickness	744:752	Focusing specifically on the composition and thickness of the hydrogel shell, permeability of the microcapsules was selectively tuned.					
36942860	4	10	theme	shell	770:774	arg1	composition					728:738	composition	728:738	composition	728:738	Focusing specifically on the composition and thickness of the hydrogel shell, permeability of the microcapsules was selectively tuned.					
36942860	2	11	theme	nutrients	334:342	arg1	transport					321:329	transport	321:329	transport of nutrients and metabolites	321:358	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	2	12	dep	size	430:433	arg1	the					426:428	the	426:428	the	426:428	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	6	13	theme	Metabolic	1078:1086	arg1	activity					1088:1095	Metabolic activity	1078:1095	Metabolic activity of spheroids in microcapsules	1078:1125	Metabolic activity of spheroids in microcapsules was confirmed by tracking the turnover of testosterone to androstenedione with chromatography studies in a metabolic assay.					
36942860	2	14	theme	Diverse	295:301	arg1	parameters					303:312	Diverse parameters	295:312	Diverse parameters	295:312	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	2	14	theme	Diverse	295:301	arg1	etc.					403:406	etc.	403:406	etc.	403:406	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	2	14	theme	Diverse	295:301	arg1	availability					361:372	availability	361:372	availability of space in the confinement	361:400	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	6	15	from	studies	1221:1227	arg1	assay					1244:1248	a metabolic assay	1232:1248	a metabolic assay	1232:1248	Metabolic activity of spheroids in microcapsules was confirmed by tracking the turnover of testosterone to androstenedione with chromatography studies in a metabolic assay.					
36942860	5	16	theme	molecules	874:882	arg1	diffusion					838:846	The diffusion	834:846	The diffusion of the selected benchmark molecules through the shell	834:900	The diffusion of the selected benchmark molecules through the shell has been systematically investigated using both, experiments and simulations, which is essential to ensure efficient mass transfer and/or filtering of the biochemical species.					
36942860	1	17	theme	models	287:292	arg1	development					248:258	the development	244:258	the development of numerous in vitro tumor models	244:292	Functional interaction between cancer cells and the surrounding microenvironment is still not sufficiently understood, which motivates the tremendous interest for the development of numerous in vitro tumor models.					
36942860	5	18	theme	efficient	1009:1017	arg1	transfer					1024:1031	efficient mass transfer	1009:1031	efficient mass transfer	1009:1031	The diffusion of the selected benchmark molecules through the shell has been systematically investigated using both, experiments and simulations, which is essential to ensure efficient mass transfer and/or filtering of the biochemical species.					
36942860	1	19	theme	Functional	81:90	arg1	interaction					92:102	Functional interaction	81:102	Functional interaction between cancer cells and the surrounding microenvironment	81:160	Functional interaction between cancer cells and the surrounding microenvironment is still not sufficiently understood, which motivates the tremendous interest for the development of numerous in vitro tumor models.					
36942860	8	20	theme	thickness	1506:1514	arg1	tuning					1486:1491	the facile tuning	1475:1491	the facile tuning of the shell thickness and permeability,	1475:1532	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	3	21	theme	low-cost	510:517	arg1	methodology					519:529	the fluidics-based low-cost methodology	491:529	the fluidics-based low-cost methodology to reproducibly generate the alginate and alginate-chitosan microcapsules	491:603	We demonstrate the fluidics-based low-cost methodology to reproducibly generate the alginate and alginate-chitosan microcapsules and apply it to grow human hepatoma (HepG2) spheroids of different dimensions and geometries.					
36942860	2	22	from	availability	361:372	arg1	confinement					390:400	the confinement	386:400	the confinement	386:400	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	6	23	theme	chromatography	1206:1219	arg1	studies					1221:1227	chromatography studies	1206:1227	chromatography studies in a metabolic assay	1206:1248	Metabolic activity of spheroids in microcapsules was confirmed by tracking the turnover of testosterone to androstenedione with chromatography studies in a metabolic assay.					
36942860	6	24	theme	spheroids	1100:1108	arg1	activity					1088:1095	Metabolic activity	1078:1095	Metabolic activity of spheroids in microcapsules	1078:1125	Metabolic activity of spheroids in microcapsules was confirmed by tracking the turnover of testosterone to androstenedione with chromatography studies in a metabolic assay.					
36942860	8	25	theme	cancer	1596:1601	arg1	spheroids					1603:1611	cancer spheroids	1596:1611	cancer spheroids	1596:1611	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	8	26	theme	permeability	1520:1531	arg1	tuning					1486:1491	the facile tuning	1475:1491	the facile tuning of the shell thickness and permeability,	1475:1532	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	6	27	from	activity	1088:1095	arg1	microcapsules					1113:1125	microcapsules	1113:1125	microcapsules	1113:1125	Metabolic activity of spheroids in microcapsules was confirmed by tracking the turnover of testosterone to androstenedione with chromatography studies in a metabolic assay.					
36942860	3	28	theme	alginate-chitosan	573:589	arg1	microcapsules					591:603	the alginate and alginate-chitosan microcapsules	556:603	the alginate and alginate-chitosan microcapsules	556:603	We demonstrate the fluidics-based low-cost methodology to reproducibly generate the alginate and alginate-chitosan microcapsules and apply it to grow human hepatoma (HepG2) spheroids of different dimensions and geometries.					
36942860	0	29	theme	soft	21:24	arg1	capsules					41:48	soft alginate-based capsules	21:48	soft alginate-based capsules for culturing liver spheroids	21:78	Shell engineering in soft alginate-based capsules for culturing liver spheroids.					
36942860	8	30	theme	facile	1479:1484	arg1	tuning					1486:1491	the facile tuning	1475:1491	the facile tuning of the shell thickness and permeability,	1475:1532	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	1	31	theme	cancer	112:117	arg1	cells					119:123	cancer cells	112:123	cancer cells	112:123	Functional interaction between cancer cells and the surrounding microenvironment is still not sufficiently understood, which motivates the tremendous interest for the development of numerous in vitro tumor models.					
36942860	8	32	with	formation	1583:1591	arg1	microenvironment					1667:1682	the surrounding microenvironment	1651:1682	the surrounding microenvironment	1651:1682	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	4	33	dep	composition	728:738	arg1	the					724:726	the	724:726	the	724:726	Focusing specifically on the composition and thickness of the hydrogel shell, permeability of the microcapsules was selectively tuned.					
36942860	3	34	theme	alginate	560:567	arg1	microcapsules					591:603	the alginate and alginate-chitosan microcapsules	556:603	the alginate and alginate-chitosan microcapsules	556:603	We demonstrate the fluidics-based low-cost methodology to reproducibly generate the alginate and alginate-chitosan microcapsules and apply it to grow human hepatoma (HepG2) spheroids of different dimensions and geometries.					
36942860	1	35	theme	tremendous	220:229	arg1	interest					231:238	the tremendous interest	216:238	the tremendous interest for the development of numerous in vitro tumor models	216:292	Functional interaction between cancer cells and the surrounding microenvironment is still not sufficiently understood, which motivates the tremendous interest for the development of numerous in vitro tumor models.					
36942860	8	36	theme	spheroids	1603:1611	arg1	interaction					1634:1644	their functional interaction	1617:1644	their functional interaction with the surrounding microenvironment	1617:1682	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	8	36	theme	spheroids	1603:1611	arg1	formation					1583:1591	the formation	1579:1591	the formation of cancer spheroids	1579:1611	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	7	37	theme	cell	1309:1312	arg1	assemblies					1314:1323	phenotypically different 3D cell assemblies	1281:1323	phenotypically different 3D cell assemblies	1281:1323	Depending on available space, phenotypically different 3D cell assemblies have been observed inside the capsules, varying in the tightness of cell aggregations and their shapes.					
36942860	5	38	theme	species	1069:1075	arg1	transfer					1024:1031	efficient mass transfer	1009:1031	efficient mass transfer	1009:1031	The diffusion of the selected benchmark molecules through the shell has been systematically investigated using both, experiments and simulations, which is essential to ensure efficient mass transfer and/or filtering of the biochemical species.					
36942860	5	39	theme	selected	855:862	arg1	molecules					874:882	the selected benchmark molecules	851:882	the selected benchmark molecules	851:882	The diffusion of the selected benchmark molecules through the shell has been systematically investigated using both, experiments and simulations, which is essential to ensure efficient mass transfer and/or filtering of the biochemical species.					
36942860	0	40	theme	alginate-based	26:39	arg1	capsules					41:48	soft alginate-based capsules	21:48	soft alginate-based capsules for culturing liver spheroids	21:78	Shell engineering in soft alginate-based capsules for culturing liver spheroids.					
36942860	8	41	theme	functional	1623:1632	arg1	interaction					1634:1644	their functional interaction	1617:1644	their functional interaction with the surrounding microenvironment	1617:1682	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	8	42	theme	shell	1500:1504	arg1	thickness					1506:1514	the shell thickness	1496:1514	the shell thickness	1496:1514	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	5	43	theme	benchmark	864:872	arg1	molecules					874:882	the selected benchmark molecules	851:882	the selected benchmark molecules	851:882	The diffusion of the selected benchmark molecules through the shell has been systematically investigated using both, experiments and simulations, which is essential to ensure efficient mass transfer and/or filtering of the biochemical species.					
36942860	0	44	theme	culturing	54:62	arg1	spheroids					70:78	culturing liver spheroids	54:78	culturing liver spheroids	54:78	Shell engineering in soft alginate-based capsules for culturing liver spheroids.					
36942860	3	45	theme	HepG2	642:646	arg1	spheroids					649:657	human hepatoma (HepG2) spheroids	626:657	human hepatoma (HepG2) spheroids of different dimensions and geometries	626:696	We demonstrate the fluidics-based low-cost methodology to reproducibly generate the alginate and alginate-chitosan microcapsules and apply it to grow human hepatoma (HepG2) spheroids of different dimensions and geometries.					
36942860	7	46	theme	shapes	1421:1426	arg1	tightness					1380:1388	the tightness	1376:1388	the tightness of cell aggregations and their shapes	1376:1426	Depending on available space, phenotypically different 3D cell assemblies have been observed inside the capsules, varying in the tightness of cell aggregations and their shapes.					
36942860	6	47	with	tracking	1144:1151	arg1	studies					1221:1227	chromatography studies	1206:1227	chromatography studies in a metabolic assay	1206:1248	Metabolic activity of spheroids in microcapsules was confirmed by tracking the turnover of testosterone to androstenedione with chromatography studies in a metabolic assay.					
36942860	6	48	theme	testosterone	1169:1180	arg1	turnover					1157:1164	the turnover	1153:1164	the turnover of testosterone to androstenedione	1153:1199	Metabolic activity of spheroids in microcapsules was confirmed by tracking the turnover of testosterone to androstenedione with chromatography studies in a metabolic assay.					
36942860	8	49	theme	promising	1547:1555	arg1	platform					1557:1564	a promising platform	1545:1564	a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment	1545:1682	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	3	50	theme	dimensions	672:681	arg1	spheroids					649:657	human hepatoma (HepG2) spheroids	626:657	human hepatoma (HepG2) spheroids of different dimensions and geometries	626:696	We demonstrate the fluidics-based low-cost methodology to reproducibly generate the alginate and alginate-chitosan microcapsules and apply it to grow human hepatoma (HepG2) spheroids of different dimensions and geometries.					
36942860	5	51	theme	biochemical	1057:1067	arg1	species					1069:1075	the biochemical species	1053:1075	the biochemical species	1053:1075	The diffusion of the selected benchmark molecules through the shell has been systematically investigated using both, experiments and simulations, which is essential to ensure efficient mass transfer and/or filtering of the biochemical species.					
36942860	2	52	theme	metabolites	348:358	arg1	transport					321:329	transport	321:329	transport of nutrients and metabolites	321:358	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	8	53	with	interaction	1634:1644	arg1	microenvironment					1667:1682	the surrounding microenvironment	1651:1682	the surrounding microenvironment	1651:1682	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	8	54	with	system	1463:1468	arg1	tuning					1486:1491	the facile tuning	1475:1491	the facile tuning of the shell thickness and permeability,	1475:1532	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	4	55	theme	microcapsules	797:809	arg1	permeability					777:788	permeability	777:788	permeability of the microcapsules	777:809	Focusing specifically on the composition and thickness of the hydrogel shell, permeability of the microcapsules was selectively tuned.					
36942860	3	56	theme	human	626:630	arg1	spheroids					649:657	human hepatoma (HepG2) spheroids	626:657	human hepatoma (HepG2) spheroids of different dimensions and geometries	626:696	We demonstrate the fluidics-based low-cost methodology to reproducibly generate the alginate and alginate-chitosan microcapsules and apply it to grow human hepatoma (HepG2) spheroids of different dimensions and geometries.					
36942860	1	57	theme	surrounding	133:143	arg1	microenvironment					145:160	the surrounding microenvironment	129:160	the surrounding microenvironment	129:160	Functional interaction between cancer cells and the surrounding microenvironment is still not sufficiently understood, which motivates the tremendous interest for the development of numerous in vitro tumor models.					
36942860	4	58	theme	hydrogel	761:768	arg1	shell					770:774	the hydrogel shell	757:774	the hydrogel shell	757:774	Focusing specifically on the composition and thickness of the hydrogel shell, permeability of the microcapsules was selectively tuned.					
36942860	8	59	theme	surrounding	1655:1665	arg1	microenvironment					1667:1682	the surrounding microenvironment	1651:1682	the surrounding microenvironment	1651:1682	Conclusively, we believe that our system with the facile tuning of the shell thickness and permeability, represents a promising platform for studying the formation of cancer spheroids and their functional interaction with the surrounding microenvironment.					
36942860	1	60	dep	in	272:273	arg1	vitro					275:279	vitro	275:279	vitro	275:279	Functional interaction between cancer cells and the surrounding microenvironment is still not sufficiently understood, which motivates the tremendous interest for the development of numerous in vitro tumor models.					
36942860	3	61	theme	hepatoma	632:639	arg1	spheroids					649:657	human hepatoma (HepG2) spheroids	626:657	human hepatoma (HepG2) spheroids of different dimensions and geometries	626:696	We demonstrate the fluidics-based low-cost methodology to reproducibly generate the alginate and alginate-chitosan microcapsules and apply it to grow human hepatoma (HepG2) spheroids of different dimensions and geometries.					
36942860	2	62	dep	transport	321:329	arg1	e.g.					315:318	e.g.	315:318	e.g.	315:318	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	3	63	theme	different	662:670	arg1	dimensions					672:681	different dimensions	662:681	different dimensions	662:681	We demonstrate the fluidics-based low-cost methodology to reproducibly generate the alginate and alginate-chitosan microcapsules and apply it to grow human hepatoma (HepG2) spheroids of different dimensions and geometries.					
36942860	7	64	theme	cell	1393:1396	arg1	aggregations					1398:1409	cell aggregations	1393:1409	cell aggregations	1393:1409	Depending on available space, phenotypically different 3D cell assemblies have been observed inside the capsules, varying in the tightness of cell aggregations and their shapes.					
36942860	3	65	theme	fluidics-based	495:508	arg1	methodology					519:529	the fluidics-based low-cost methodology	491:529	the fluidics-based low-cost methodology to reproducibly generate the alginate and alginate-chitosan microcapsules	491:603	We demonstrate the fluidics-based low-cost methodology to reproducibly generate the alginate and alginate-chitosan microcapsules and apply it to grow human hepatoma (HepG2) spheroids of different dimensions and geometries.					
36942860	0	66	theme	liver	64:68	arg1	spheroids					70:78	culturing liver spheroids	54:78	culturing liver spheroids	54:78	Shell engineering in soft alginate-based capsules for culturing liver spheroids.					
36942860	7	67	theme	aggregations	1398:1409	arg1	tightness					1380:1388	the tightness	1376:1388	the tightness of cell aggregations and their shapes	1376:1426	Depending on available space, phenotypically different 3D cell assemblies have been observed inside the capsules, varying in the tightness of cell aggregations and their shapes.					
36942860	2	68	theme	space	377:381	arg1	parameters					303:312	Diverse parameters	295:312	Diverse parameters	295:312	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	2	68	theme	space	377:381	arg1	availability					361:372	availability	361:372	availability of space in the confinement	361:400	Diverse parameters, e.g., transport of nutrients and metabolites, availability of space in the confinement, etc. make an impact on the size, shape, and metabolism of the tumoroids.					
36942860	1	69	theme	numerous	263:270	arg1	models					287:292	numerous in vitro tumor models	263:292	numerous in vitro tumor models	263:292	Functional interaction between cancer cells and the surrounding microenvironment is still not sufficiently understood, which motivates the tremendous interest for the development of numerous in vitro tumor models.					
36870656	6	0	from	temperature	853:863	arg1	stability					821:829	the thermal stability	809:829	the thermal stability of OSA starch at high temperature	809:863	TGA indicated that FA effectively enhanced the thermal stability of OSA starch at high temperature.					
36870656	6	1	theme	high	848:851	arg1	temperature					853:863	high temperature	848:863	high temperature	848:863	TGA indicated that FA effectively enhanced the thermal stability of OSA starch at high temperature.					
36870656	10	2	theme	loading	1327:1333	arg1	DOX					1335:1337	loading DOX	1327:1337	loading DOX	1327:1337	These results provide novel insights into OSA starch grafted with FA as potential strategy for loading DOX.					
36870656	1	3	theme	octenyl	145:151	arg1	OSA					173:175	OSA	173:175	OSA	173:175	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	1	3	theme	octenyl	145:151	arg1	anhydride					162:170	octenyl succinic anhydride	145:170	octenyl succinic anhydride (OSA)	145:176	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	4	4	theme	surface	545:551	arg1	roughness					553:561	the surface roughness	541:561	the surface roughness of OSA starch granules	541:584	SEM images showed that the surface roughness of OSA starch granules was more obvious with higher FA grafting time.					
36870656	4	4	theme	surface	545:551	arg1	obvious					595:601	obvious	595:601	obvious	595:601	SEM images showed that the surface roughness of OSA starch granules was more obvious with higher FA grafting time.					
36870656	7	5	theme	FA	985:986	arg1	reaction					997:1004	FA grafting reaction	985:1004	FA grafting reaction	985:1004	The crystalline form of the OSA starch gradually transformed from A type to a hybrid A and V-type with the progress of FA grafting reaction.					
36870656	9	6	theme	starch	1184:1189	arg1	efficiency					1166:1175	the loading efficiency	1154:1175	the loading efficiency of OSA starch grafted with FA for DOX	1154:1213	Using doxorubicin hydrochloride (DOX) as the model drug, the loading efficiency of OSA starch grafted with FA for DOX reached 87.71 %.					
36870656	9	7	theme	loading	1158:1164	arg1	efficiency					1166:1175	the loading efficiency	1154:1175	the loading efficiency of OSA starch grafted with FA for DOX	1154:1213	Using doxorubicin hydrochloride (DOX) as the model drug, the loading efficiency of OSA starch grafted with FA for DOX reached 87.71 %.					
36870656	3	8	from	introduction	475:486	arg1	granules					508:515	OSA starch granules	497:515	OSA starch granules	497:515	FTIR spectra further confirmed the successful introduction of FA on OSA starch granules.					
36870656	1	9	theme	succinic	153:160	arg1	OSA					173:175	OSA	173:175	OSA	173:175	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	1	9	theme	succinic	153:160	arg1	anhydride					162:170	octenyl succinic anhydride	145:170	octenyl succinic anhydride (OSA)	145:176	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	10	10	theme	novel	1254:1258	arg1	insights					1260:1267	novel insights	1254:1267	novel insights into OSA starch grafted with FA as potential strategy for loading DOX	1254:1337	These results provide novel insights into OSA starch grafted with FA as potential strategy for loading DOX.					
36870656	5	11	theme	swelling	672:679	arg1	properties					681:690	The particle size, zeta potential, and swelling properties	633:690	The particle size, zeta potential, and swelling properties	633:690	The particle size, zeta potential, and swelling properties were determined to study the effect of FA on the structure of OSA starch.					
36870656	6	12	theme	starch	838:843	arg1	stability					821:829	the thermal stability	809:829	the thermal stability of OSA starch at high temperature	809:863	TGA indicated that FA effectively enhanced the thermal stability of OSA starch at high temperature.					
36870656	7	13	theme	starch	898:903	arg1	form					882:885	The crystalline form	866:885	The crystalline form of the OSA starch	866:903	The crystalline form of the OSA starch gradually transformed from A type to a hybrid A and V-type with the progress of FA grafting reaction.					
36870656	0	14	theme	loading	81:87	arg1	potential					89:97	its loading potential	77:97	its loading potential for doxorubicin hydrochloride	77:127	Fabrication of octenyl succinic anhydride starch grafted with folic acid and its loading potential for doxorubicin hydrochloride.					
36870656	6	15	theme	OSA	834:836	arg1	starch					838:843	OSA starch	834:843	OSA starch	834:843	TGA indicated that FA effectively enhanced the thermal stability of OSA starch at high temperature.					
36870656	10	16	theme	potential	1304:1312	arg1	strategy					1314:1321	potential strategy	1304:1321	potential strategy for loading DOX	1304:1337	These results provide novel insights into OSA starch grafted with FA as potential strategy for loading DOX.					
36870656	7	17	dep	A	951:951	arg1	a					942:942	a	942:942	a	942:942	The crystalline form of the OSA starch gradually transformed from A type to a hybrid A and V-type with the progress of FA grafting reaction.					
36870656	0	18	theme	doxorubicin	103:113	arg1	hydrochloride					115:127	doxorubicin hydrochloride	103:127	doxorubicin hydrochloride	103:127	Fabrication of octenyl succinic anhydride starch grafted with folic acid and its loading potential for doxorubicin hydrochloride.					
36870656	1	19	from	degree	251:256	arg1	time					299:302	different grafting time	280:302	different grafting time	280:302	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	9	20	theme	doxorubicin	1103:1113	arg1	drug					1148:1151	the model drug	1138:1151	the model drug	1138:1151	Using doxorubicin hydrochloride (DOX) as the model drug, the loading efficiency of OSA starch grafted with FA for DOX reached 87.71 %.					
36870656	9	20	theme	doxorubicin	1103:1113	arg1	DOX					1130:1132	DOX	1130:1132	DOX	1130:1132	Using doxorubicin hydrochloride (DOX) as the model drug, the loading efficiency of OSA starch grafted with FA for DOX reached 87.71 %.					
36870656	9	20	theme	doxorubicin	1103:1113	arg1	hydrochloride					1115:1127	doxorubicin hydrochloride	1103:1127	doxorubicin hydrochloride (DOX)	1103:1133	Using doxorubicin hydrochloride (DOX) as the model drug, the loading efficiency of OSA starch grafted with FA for DOX reached 87.71 %.					
36870656	3	21	theme	OSA	497:499	arg1	granules					508:515	OSA starch granules	497:515	OSA starch granules	497:515	FTIR spectra further confirmed the successful introduction of FA on OSA starch granules.					
36870656	7	22	with	V-type	957:962	arg1	progress					973:980	the progress	969:980	the progress of FA grafting reaction	969:1004	The crystalline form of the OSA starch gradually transformed from A type to a hybrid A and V-type with the progress of FA grafting reaction.					
36870656	7	23	with	A	951:951	arg1	progress					973:980	the progress	969:980	the progress of FA grafting reaction	969:1004	The crystalline form of the OSA starch gradually transformed from A type to a hybrid A and V-type with the progress of FA grafting reaction.					
36870656	7	24	theme	crystalline	870:880	arg1	form					882:885	The crystalline form	866:885	The crystalline form of the OSA starch	866:903	The crystalline form of the OSA starch gradually transformed from A type to a hybrid A and V-type with the progress of FA grafting reaction.					
36870656	3	25	theme	starch	501:506	arg1	granules					508:515	OSA starch granules	497:515	OSA starch granules	497:515	FTIR spectra further confirmed the successful introduction of FA on OSA starch granules.					
36870656	9	26	theme	model	1142:1146	arg1	drug					1148:1151	the model drug	1138:1151	the model drug	1138:1151	Using doxorubicin hydrochloride (DOX) as the model drug, the loading efficiency of OSA starch grafted with FA for DOX reached 87.71 %.					
36870656	9	26	theme	model	1142:1146	arg1	hydrochloride					1115:1127	doxorubicin hydrochloride	1103:1127	doxorubicin hydrochloride (DOX)	1103:1133	Using doxorubicin hydrochloride (DOX) as the model drug, the loading efficiency of OSA starch grafted with FA for DOX reached 87.71 %.					
36870656	6	27	theme	thermal	813:819	arg1	stability					821:829	the thermal stability	809:829	the thermal stability of OSA starch at high temperature	809:863	TGA indicated that FA effectively enhanced the thermal stability of OSA starch at high temperature.					
36870656	7	28	theme	grafting	988:995	arg1	reaction					997:1004	FA grafting reaction	985:1004	FA grafting reaction	985:1004	The crystalline form of the OSA starch gradually transformed from A type to a hybrid A and V-type with the progress of FA grafting reaction.					
36870656	4	29	theme	higher	608:613	arg1	FA					615:616	higher FA	608:616	higher FA grafting time	608:630	SEM images showed that the surface roughness of OSA starch granules was more obvious with higher FA grafting time.					
36870656	7	30	theme	reaction	997:1004	arg1	progress					973:980	the progress	969:980	the progress of FA grafting reaction	969:1004	The crystalline form of the OSA starch gradually transformed from A type to a hybrid A and V-type with the progress of FA grafting reaction.					
36870656	8	31	theme	starch	1057:1062	arg1	properties					1039:1048	the anti-digestive properties	1020:1048	the anti-digestive properties of OSA starch	1020:1062	In addition, the anti-digestive properties of OSA starch were enhanced after grafting FA.					
36870656	2	32	theme	XPS	335:337	arg1	results					324:330	The results	320:330	The results of XPS	320:337	The results of XPS quantitatively reflected the surface elemental composition of OSA starch grafted with FA.					
36870656	0	33	theme	succinic	23:30	arg1	anhydride					32:40	octenyl succinic anhydride	15:40	octenyl succinic anhydride starch	15:47	Fabrication of octenyl succinic anhydride starch grafted with folic acid and its loading potential for doxorubicin hydrochloride.					
36870656	4	34	theme	SEM	518:520	arg1	images					522:527	SEM images	518:527	SEM images	518:527	SEM images showed that the surface roughness of OSA starch granules was more obvious with higher FA grafting time.					
36870656	5	35	theme	zeta	652:655	arg1	potential					657:665	zeta potential	652:665	zeta potential	652:665	The particle size, zeta potential, and swelling properties were determined to study the effect of FA on the structure of OSA starch.					
36870656	7	36	theme	OSA	894:896	arg1	starch					898:903	the OSA starch	890:903	the OSA starch	890:903	The crystalline form of the OSA starch gradually transformed from A type to a hybrid A and V-type with the progress of FA grafting reaction.					
36870656	1	37	theme	starch	178:183	arg1	time					225:228	octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time	145:228	octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time	145:228	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	2	38	theme	OSA	401:403	arg1	starch					405:410	OSA starch	401:410	OSA starch grafted with FA	401:426	The results of XPS quantitatively reflected the surface elemental composition of OSA starch grafted with FA.					
36870656	5	39	theme	potential	657:665	arg1	properties					681:690	The particle size, zeta potential, and swelling properties	633:690	The particle size, zeta potential, and swelling properties	633:690	The particle size, zeta potential, and swelling properties were determined to study the effect of FA on the structure of OSA starch.					
36870656	5	40	theme	OSA	754:756	arg1	starch					758:763	OSA starch	754:763	OSA starch	754:763	The particle size, zeta potential, and swelling properties were determined to study the effect of FA on the structure of OSA starch.					
36870656	0	41	theme	starch	42:47	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of octenyl succinic anhydride starch	0:47	Fabrication of octenyl succinic anhydride starch grafted with folic acid and its loading potential for doxorubicin hydrochloride.					
36870656	1	42	with	starch	178:183	arg1	grafting					216:223	different folic acid (FA) grafting	190:223	different folic acid (FA) grafting	190:223	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	2	43	theme	elemental	376:384	arg1	composition					386:396	the surface elemental composition	364:396	the surface elemental composition of OSA starch grafted with FA	364:426	The results of XPS quantitatively reflected the surface elemental composition of OSA starch grafted with FA.					
36870656	1	44	theme	different	190:198	arg1	FA					212:213	FA	212:213	FA	212:213	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	1	44	theme	different	190:198	arg1	acid					206:209	different folic acid	190:209	different folic acid (FA) grafting	190:223	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	0	45	theme	anhydride	32:40	arg1	starch					42:47	octenyl succinic anhydride starch	15:47	octenyl succinic anhydride starch	15:47	Fabrication of octenyl succinic anhydride starch grafted with folic acid and its loading potential for doxorubicin hydrochloride.					
36870656	1	46	theme	FA	261:262	arg1	substitution					264:275	FA substitution	261:275	FA substitution at different grafting time	261:302	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	5	47	theme	FA	731:732	arg1	effect					721:726	the effect	717:726	the effect of FA on the structure of OSA starch	717:763	The particle size, zeta potential, and swelling properties were determined to study the effect of FA on the structure of OSA starch.					
36870656	8	48	theme	OSA	1053:1055	arg1	starch					1057:1062	OSA starch	1053:1062	OSA starch	1053:1062	In addition, the anti-digestive properties of OSA starch were enhanced after grafting FA.					
36870656	2	49	theme	starch	405:410	arg1	composition					386:396	the surface elemental composition	364:396	the surface elemental composition of OSA starch grafted with FA	364:426	The results of XPS quantitatively reflected the surface elemental composition of OSA starch grafted with FA.					
36870656	1	50	theme	folic	200:204	arg1	FA					212:213	FA	212:213	FA	212:213	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	1	50	theme	folic	200:204	arg1	acid					206:209	different folic acid	190:209	different folic acid (FA) grafting	190:223	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	1	51	theme	substitution	264:275	arg1	degree					251:256	the degree	247:256	the degree of FA substitution at different grafting time	247:302	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	7	52	theme	hybrid	944:949	arg1	A					951:951	hybrid A	944:951	hybrid A	944:951	The crystalline form of the OSA starch gradually transformed from A type to a hybrid A and V-type with the progress of FA grafting reaction.					
36870656	1	53	from	time	299:302	arg1	degree					251:256	the degree	247:256	the degree of FA substitution at different grafting time	247:302	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	1	53	from	time	299:302	arg1	substitution					264:275	FA substitution	261:275	FA substitution at different grafting time	261:302	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	5	54	theme	particle	637:644	arg1	size					646:649	particle size	637:649	particle size	637:649	The particle size, zeta potential, and swelling properties were determined to study the effect of FA on the structure of OSA starch.					
36870656	9	55	theme	OSA	1180:1182	arg1	starch					1184:1189	OSA starch	1180:1189	OSA starch grafted with FA	1180:1205	Using doxorubicin hydrochloride (DOX) as the model drug, the loading efficiency of OSA starch grafted with FA for DOX reached 87.71 %.					
36870656	3	56	theme	successful	464:473	arg1	introduction					475:486	the successful introduction	460:486	the successful introduction of FA on OSA starch granules	460:515	FTIR spectra further confirmed the successful introduction of FA on OSA starch granules.					
36870656	1	57	theme	different	280:288	arg1	grafting					290:297	different grafting	280:297	different grafting time	280:302	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	5	58	theme	size	646:649	arg1	properties					681:690	The particle size, zeta potential, and swelling properties	633:690	The particle size, zeta potential, and swelling properties	633:690	The particle size, zeta potential, and swelling properties were determined to study the effect of FA on the structure of OSA starch.					
36870656	5	59	from	effect	721:726	arg1	structure					741:749	the structure	737:749	the structure of OSA starch	737:763	The particle size, zeta potential, and swelling properties were determined to study the effect of FA on the structure of OSA starch.					
36870656	10	60	theme	OSA	1274:1276	arg1	starch					1278:1283	OSA starch	1274:1283	OSA starch grafted with FA as potential strategy for loading DOX	1274:1337	These results provide novel insights into OSA starch grafted with FA as potential strategy for loading DOX.					
36870656	1	61	theme	acid	206:209	arg1	grafting					216:223	different folic acid (FA) grafting	190:223	different folic acid (FA) grafting	190:223	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	4	62	with	obvious	595:601	arg1	FA					615:616	higher FA	608:616	higher FA grafting time	608:630	SEM images showed that the surface roughness of OSA starch granules was more obvious with higher FA grafting time.					
36870656	5	63	theme	starch	758:763	arg1	structure					741:749	the structure	737:749	the structure of OSA starch	737:763	The particle size, zeta potential, and swelling properties were determined to study the effect of FA on the structure of OSA starch.					
36870656	8	64	theme	anti-digestive	1024:1037	arg1	properties					1039:1048	the anti-digestive properties	1020:1048	the anti-digestive properties of OSA starch	1020:1062	In addition, the anti-digestive properties of OSA starch were enhanced after grafting FA.					
36870656	4	65	theme	OSA	566:568	arg1	granules					577:584	OSA starch granules	566:584	OSA starch granules	566:584	SEM images showed that the surface roughness of OSA starch granules was more obvious with higher FA grafting time.					
36870656	2	66	theme	surface	368:374	arg1	composition					386:396	the surface elemental composition	364:396	the surface elemental composition of OSA starch grafted with FA	364:426	The results of XPS quantitatively reflected the surface elemental composition of OSA starch grafted with FA.					
36870656	3	67	theme	FTIR	429:432	arg1	spectra					434:440	FTIR spectra	429:440	FTIR spectra	429:440	FTIR spectra further confirmed the successful introduction of FA on OSA starch granules.					
36870656	3	68	theme	FA	491:492	arg1	introduction					475:486	the successful introduction	460:486	the successful introduction of FA on OSA starch granules	460:515	FTIR spectra further confirmed the successful introduction of FA on OSA starch granules.					
36870656	0	69	theme	folic	62:66	arg1	acid					68:71	folic acid	62:71	folic acid	62:71	Fabrication of octenyl succinic anhydride starch grafted with folic acid and its loading potential for doxorubicin hydrochloride.					
36870656	1	70	theme	grafting	290:297	arg1	time					299:302	different grafting time	280:302	different grafting time	280:302	In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined.					
36870656	0	71	theme	octenyl	15:21	arg1	anhydride					32:40	octenyl succinic anhydride	15:40	octenyl succinic anhydride starch	15:47	Fabrication of octenyl succinic anhydride starch grafted with folic acid and its loading potential for doxorubicin hydrochloride.					
36870656	4	72	theme	starch	570:575	arg1	granules					577:584	OSA starch granules	566:584	OSA starch granules	566:584	SEM images showed that the surface roughness of OSA starch granules was more obvious with higher FA grafting time.					
36870656	4	73	theme	granules	577:584	arg1	roughness					553:561	the surface roughness	541:561	the surface roughness of OSA starch granules	541:584	SEM images showed that the surface roughness of OSA starch granules was more obvious with higher FA grafting time.					
36870656	4	73	theme	granules	577:584	arg1	obvious					595:601	obvious	595:601	obvious	595:601	SEM images showed that the surface roughness of OSA starch granules was more obvious with higher FA grafting time.					
36129764	8	0	theme	mass	1382:1385	arg1	spectrometry					1387:1398	high-resolution mass spectrometry	1366:1398	high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed	1366:1630	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	6	1	theme	wide	1033:1036	arg1	Clavibacter					1084:1094	Clavibacter	1084:1094	Clavibacter	1084:1094	A wide range of plant pathogenic bacteria, including Clavibacter michiganensis subsp.					
36129764	6	1	theme	wide	1033:1036	arg1	range					1038:1042	A wide range	1031:1042	A wide range of plant pathogenic bacteria, including Clavibacter	1031:1094	A wide range of plant pathogenic bacteria, including Clavibacter michiganensis subsp.					
36129764	9	2	theme	N-butanoyl-L-homoserine	1695:1717	arg1	lactone					1765:1771	N-(3-hydroxy-butanoyl)-homoserine lactone	1731:1771	N-(3-hydroxy-butanoyl)-homoserine lactone	1731:1771	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	2	theme	N-butanoyl-L-homoserine	1695:1717	arg1	metabolites					1805:1815	known quorum-sensing metabolites	1784:1815	known quorum-sensing metabolites in other bacteria	1784:1833	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	2	theme	N-butanoyl-L-homoserine	1695:1717	arg1	lactone					1719:1725	N-butanoyl-L-homoserine lactone	1695:1725	N-butanoyl-L-homoserine lactone	1695:1725	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	3	3	theme	Petri	606:610	arg1	plates					612:617	Petri plates	606:617	Petri plates containing multiple spots	606:643	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
36129764	2	4	theme	X.	357:358	arg1	euvesicatoria					360:372	X. euvesicatoria	357:372	X. euvesicatoria	357:372	X. perforans quickly replaced X. euvesicatoria, mainly because of production of three bacteriocins (BCNs) against X. euvesicatoria; however, X. perforans outcompeted X. euvesicatoria even when the three known BCNs were deleted.					
36129764	9	5	theme	known	1784:1788	arg1	lactone					1765:1771	N-(3-hydroxy-butanoyl)-homoserine lactone	1731:1771	N-(3-hydroxy-butanoyl)-homoserine lactone	1731:1771	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	5	theme	known	1784:1788	arg1	metabolites					1805:1815	known quorum-sensing metabolites	1784:1815	known quorum-sensing metabolites in other bacteria	1784:1833	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	5	theme	known	1784:1788	arg1	lactone					1719:1725	N-butanoyl-L-homoserine lactone	1695:1725	N-butanoyl-L-homoserine lactone	1695:1725	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	8	6	with	plates	1565:1570	arg1	spot					1581:1584	one spot	1577:1584	one spot	1577:1584	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	5	7	theme	Other	872:876	arg1	species					890:896	Other Xanthomonas species	872:896	Other Xanthomonas species	872:896	Other Xanthomonas species also produced volatile-induced antimicrobial compounds against X. euvesicatoria and elicited antimicrobial activity by X. perforans.					
36129764	5	8	theme	Xanthomonas	878:888	arg1	species					890:896	Other Xanthomonas species	872:896	Other Xanthomonas species	872:896	Other Xanthomonas species also produced volatile-induced antimicrobial compounds against X. euvesicatoria and elicited antimicrobial activity by X. perforans.					
36129764	8	9	theme	Petri	1559:1563	arg1	plates					1565:1570	Petri plates	1559:1570	Petri plates with one spot	1559:1584	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	7	10	theme	multiple	1235:1242	arg1	spots					1244:1248	multiple spots	1235:1248	multiple spots of the species	1235:1263	michiganensis, Pantoea stewartii, and Pseudomonas cichorii, also elicited antimicrobial activity by X. perforans when multiple spots of the species were present.					
36129764	0	11	from	Strength	0:7	arg1	Numbers					12:18	Numbers	12:18	Numbers	12:18	Strength in Numbers: Density-Dependent Volatile-Induced Antimicrobial Activity by Xanthomonas perforans.					
36129764	4	12	theme	antimicrobial	848:860	arg1	activity					862:869	the antimicrobial activity	844:869	the antimicrobial activity	844:869	We determined that changes in the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar were required for induction of the antimicrobial activity.					
36129764	3	13	from	activity	511:518	arg1	mutant					563:568	the BCN triple mutant	548:568	the BCN triple mutant	548:568	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
36129764	9	14	theme	3-hydroxy-butanoyl	1734:1751	arg1	lactone					1765:1771	N-(3-hydroxy-butanoyl)-homoserine lactone	1731:1771	N-(3-hydroxy-butanoyl)-homoserine lactone	1731:1771	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	14	theme	3-hydroxy-butanoyl	1734:1751	arg1	metabolites					1805:1815	known quorum-sensing metabolites	1784:1815	known quorum-sensing metabolites in other bacteria	1784:1833	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	14	theme	3-hydroxy-butanoyl	1734:1751	arg1	lactone					1719:1725	N-butanoyl-L-homoserine lactone	1695:1725	N-butanoyl-L-homoserine lactone	1695:1725	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	7	15	dep	X.	1217:1218	arg1	perforans					1220:1228	X. perforans	1217:1228	X. perforans	1217:1228	michiganensis, Pantoea stewartii, and Pseudomonas cichorii, also elicited antimicrobial activity by X. perforans when multiple spots of the species were present.					
36129764	3	16	theme	BCN	552:554	arg1	mutant					563:568	the BCN triple mutant	548:568	the BCN triple mutant	548:568	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
36129764	2	17	theme	bacteriocins	329:340	arg1	production					309:318	production	309:318	production of three bacteriocins (BCNs) against X. euvesicatoria	309:372	X. perforans quickly replaced X. euvesicatoria, mainly because of production of three bacteriocins (BCNs) against X. euvesicatoria; however, X. perforans outcompeted X. euvesicatoria even when the three known BCNs were deleted.					
36129764	8	18	theme	potential	1291:1299	arg1	compounds					1315:1323	potential antimicrobial compounds	1291:1323	potential antimicrobial compounds	1291:1323	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	5	19	theme	antimicrobial	991:1003	arg1	activity					1005:1012	antimicrobial activity	991:1012	antimicrobial activity	991:1012	Other Xanthomonas species also produced volatile-induced antimicrobial compounds against X. euvesicatoria and elicited antimicrobial activity by X. perforans.					
36129764	1	20	theme	known	174:178	arg1	bacterium					180:188	the only known bacterium	165:188	the only known bacterium associated with bacterial spot of tomato in Florida	165:240	For most of the 20th century, Xanthomonas euvesicatoria was the only known bacterium associated with bacterial spot of tomato in Florida.					
36129764	1	20	theme	known	174:178	arg1	euvesicatoria					147:159	Xanthomonas euvesicatoria	135:159	Xanthomonas euvesicatoria	135:159	For most of the 20th century, Xanthomonas euvesicatoria was the only known bacterium associated with bacterial spot of tomato in Florida.					
36129764	8	21	theme	high-resolution	1366:1380	arg1	spectrometry					1387:1398	high-resolution mass spectrometry	1366:1398	high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed	1366:1630	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	7	22	theme	Pantoea	1132:1138	arg1	stewartii					1140:1148	Pantoea stewartii	1132:1148	Pantoea stewartii	1132:1148	michiganensis, Pantoea stewartii, and Pseudomonas cichorii, also elicited antimicrobial activity by X. perforans when multiple spots of the species were present.					
36129764	8	23	theme	agar	1407:1410	arg1	spectrometry					1387:1398	high-resolution mass spectrometry	1366:1398	high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed	1366:1630	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	2	24	theme	known	446:450	arg1	BCNs					452:455	the three known BCNs	436:455	the three known BCNs	436:455	X. perforans quickly replaced X. euvesicatoria, mainly because of production of three bacteriocins (BCNs) against X. euvesicatoria; however, X. perforans outcompeted X. euvesicatoria even when the three known BCNs were deleted.					
36129764	7	25	theme	species	1257:1263	arg1	spots					1244:1248	multiple spots	1235:1248	multiple spots of the species	1235:1263	michiganensis, Pantoea stewartii, and Pseudomonas cichorii, also elicited antimicrobial activity by X. perforans when multiple spots of the species were present.					
36129764	5	26	theme	antimicrobial	929:941	arg1	compounds					943:951	volatile-induced antimicrobial compounds	912:951	volatile-induced antimicrobial compounds against X. euvesicatoria	912:976	Other Xanthomonas species also produced volatile-induced antimicrobial compounds against X. euvesicatoria and elicited antimicrobial activity by X. perforans.					
36129764	9	27	theme	quorum-sensing	1790:1803	arg1	lactone					1765:1771	N-(3-hydroxy-butanoyl)-homoserine lactone	1731:1771	N-(3-hydroxy-butanoyl)-homoserine lactone	1731:1771	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	27	theme	quorum-sensing	1790:1803	arg1	metabolites					1805:1815	known quorum-sensing metabolites	1784:1815	known quorum-sensing metabolites in other bacteria	1784:1833	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	27	theme	quorum-sensing	1790:1803	arg1	lactone					1719:1725	N-butanoyl-L-homoserine lactone	1695:1725	N-butanoyl-L-homoserine lactone	1695:1725	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	3	28	contain	containing	669:678	arg1	plates					662:667	Petri plates	656:667	Petri plates containing only one spot	656:692	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
36129764	3	28	contain	containing	669:678	arg2	spot					689:692	only one spot	680:692	only one spot	680:692	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
36129764	9	29	theme	-homoserine	1753:1763	arg1	lactone					1765:1771	N-(3-hydroxy-butanoyl)-homoserine lactone	1731:1771	N-(3-hydroxy-butanoyl)-homoserine lactone	1731:1771	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	29	theme	-homoserine	1753:1763	arg1	metabolites					1805:1815	known quorum-sensing metabolites	1784:1815	known quorum-sensing metabolites in other bacteria	1784:1833	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	29	theme	-homoserine	1753:1763	arg1	lactone					1719:1725	N-butanoyl-L-homoserine lactone	1695:1725	N-butanoyl-L-homoserine lactone	1695:1725	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	30	theme	other	1820:1824	arg1	bacteria					1826:1833	other bacteria	1820:1833	other bacteria	1820:1833	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	7	31	theme	antimicrobial	1191:1203	arg1	activity					1205:1212	antimicrobial activity	1191:1212	antimicrobial activity	1191:1212	michiganensis, Pantoea stewartii, and Pseudomonas cichorii, also elicited antimicrobial activity by X. perforans when multiple spots of the species were present.					
36129764	8	32	theme	antimicrobial	1592:1604	arg1	activity					1606:1613	antimicrobial activity	1592:1613	antimicrobial activity	1592:1613	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	8	33	theme	liquid	1339:1344	arg1	chromatography					1346:1359	liquid chromatography	1339:1359	liquid chromatography	1339:1359	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	5	34	theme	volatile-induced	912:927	arg1	compounds					943:951	volatile-induced antimicrobial compounds	912:951	volatile-induced antimicrobial compounds against X. euvesicatoria	912:976	Other Xanthomonas species also produced volatile-induced antimicrobial compounds against X. euvesicatoria and elicited antimicrobial activity by X. perforans.					
36129764	3	35	theme	triple	556:561	arg1	mutant					563:568	the BCN triple mutant	548:568	the BCN triple mutant	548:568	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
36129764	8	36	theme	cell	1445:1448	arg1	density					1450:1456	high cell density	1440:1456	the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed	1436:1630	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	1	37	theme	bacterial	206:214	arg1	spot					216:219	bacterial spot	206:219	bacterial spot of tomato in Florida	206:240	For most of the 20th century, Xanthomonas euvesicatoria was the only known bacterium associated with bacterial spot of tomato in Florida.					
36129764	8	38	from	spot	1551:1554	arg1	plates					1565:1570	Petri plates	1559:1570	Petri plates with one spot	1559:1584	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	8	39	from	spot	1428:1431	arg1	plates					1464:1469	the high cell density Petri plates	1436:1469	the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed	1436:1630	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	8	40	theme	high	1440:1443	arg1	density					1450:1456	high cell density	1440:1456	the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed	1436:1630	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	4	41	theme	headspace	729:737	arg1	composition					739:749	the headspace composition	725:749	the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar	725:811	We determined that changes in the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar were required for induction of the antimicrobial activity.					
36129764	4	41	theme	headspace	729:737	arg1	volatiles					758:766	volatiles	758:766	volatiles	758:766	We determined that changes in the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar were required for induction of the antimicrobial activity.					
36129764	6	42	theme	bacteria	1064:1071	arg1	Clavibacter					1084:1094	Clavibacter	1084:1094	Clavibacter	1084:1094	A wide range of plant pathogenic bacteria, including Clavibacter michiganensis subsp.					
36129764	6	42	theme	bacteria	1064:1071	arg1	range					1038:1042	A wide range	1031:1042	A wide range of plant pathogenic bacteria, including Clavibacter	1031:1094	A wide range of plant pathogenic bacteria, including Clavibacter michiganensis subsp.					
36129764	0	43	theme	Volatile-Induced	39:54	arg1	Activity					70:77	Density-Dependent Volatile-Induced Antimicrobial Activity	21:77	Strength in Numbers: Density-Dependent Volatile-Induced Antimicrobial Activity by Xanthomonas perforans.	0:103	Strength in Numbers: Density-Dependent Volatile-Induced Antimicrobial Activity by Xanthomonas perforans.					
36129764	6	44	theme	pathogenic	1053:1062	arg1	bacteria					1064:1071	plant pathogenic bacteria	1047:1071	plant pathogenic bacteria	1047:1071	A wide range of plant pathogenic bacteria, including Clavibacter michiganensis subsp.					
36129764	6	44	theme	pathogenic	1053:1062	arg1	Clavibacter					1084:1094	Clavibacter	1084:1094	Clavibacter	1084:1094	A wide range of plant pathogenic bacteria, including Clavibacter michiganensis subsp.					
36129764	2	45	theme	X.	243:244	arg1	perforans					246:254	X. perforans	243:254	X. perforans	243:254	X. perforans quickly replaced X. euvesicatoria, mainly because of production of three bacteriocins (BCNs) against X. euvesicatoria; however, X. perforans outcompeted X. euvesicatoria even when the three known BCNs were deleted.					
36129764	2	46	theme	X.	409:410	arg1	euvesicatoria					412:424	X. euvesicatoria	409:424	X. euvesicatoria	409:424	X. perforans quickly replaced X. euvesicatoria, mainly because of production of three bacteriocins (BCNs) against X. euvesicatoria; however, X. perforans outcompeted X. euvesicatoria even when the three known BCNs were deleted.					
36129764	0	47	theme	Density-Dependent	21:37	arg1	Activity					70:77	Density-Dependent Volatile-Induced Antimicrobial Activity	21:77	Strength in Numbers: Density-Dependent Volatile-Induced Antimicrobial Activity by Xanthomonas perforans.	0:103	Strength in Numbers: Density-Dependent Volatile-Induced Antimicrobial Activity by Xanthomonas perforans.					
36129764	3	48	theme	multiple	630:637	arg1	spots					639:643	multiple spots	630:643	multiple spots	630:643	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
36129764	4	49	theme	diffusible	783:792	arg1	signal					794:799	a diffusible signal	781:799	the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar	725:811	We determined that changes in the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar were required for induction of the antimicrobial activity.					
36129764	9	50	theme	inhibition	1679:1688	arg1	zone					1671:1674	the zone	1667:1674	the zone of inhibition	1667:1688	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	1	51	theme	tomato	224:229	arg1	spot					216:219	bacterial spot	206:219	bacterial spot of tomato in Florida	206:240	For most of the 20th century, Xanthomonas euvesicatoria was the only known bacterium associated with bacterial spot of tomato in Florida.					
36129764	3	52	theme	antimicrobial	497:509	arg1	activity					511:518	antimicrobial activity	497:518	antimicrobial activity against X. euvesicatoria in the BCN triple mutant	497:568	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
36129764	6	53	theme	plant	1047:1051	arg1	bacteria					1064:1071	plant pathogenic bacteria	1047:1071	plant pathogenic bacteria	1047:1071	A wide range of plant pathogenic bacteria, including Clavibacter michiganensis subsp.					
36129764	6	53	theme	plant	1047:1051	arg1	Clavibacter					1084:1094	Clavibacter	1084:1094	Clavibacter	1084:1094	A wide range of plant pathogenic bacteria, including Clavibacter michiganensis subsp.					
36129764	3	54	theme	triple	579:584	arg1	mutant					586:591	the triple mutant	575:591	the triple mutant	575:591	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
36129764	1	55	theme	20th	121:124	arg1	century					126:132	the 20th century	117:132	the 20th century	117:132	For most of the 20th century, Xanthomonas euvesicatoria was the only known bacterium associated with bacterial spot of tomato in Florida.					
36129764	0	56	theme	Antimicrobial	56:68	arg1	Activity					70:77	Density-Dependent Volatile-Induced Antimicrobial Activity	21:77	Strength in Numbers: Density-Dependent Volatile-Induced Antimicrobial Activity by Xanthomonas perforans.	0:103	Strength in Numbers: Density-Dependent Volatile-Induced Antimicrobial Activity by Xanthomonas perforans.					
36129764	8	57	theme	Petri	1458:1462	arg1	plates					1464:1469	the high cell density Petri plates	1436:1469	the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed	1436:1630	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	4	58	theme	activity	862:869	arg1	induction					831:839	induction	831:839	induction of the antimicrobial activity	831:869	We determined that changes in the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar were required for induction of the antimicrobial activity.					
36129764	8	59	theme	density	1450:1456	arg1	plates					1464:1469	the high cell density Petri plates	1436:1469	the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed	1436:1630	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	4	60	from	signal	794:799	arg1	agar					808:811	the agar	804:811	the agar	804:811	We determined that changes in the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar were required for induction of the antimicrobial activity.					
36129764	4	61	from	changes	714:720	arg1	signal					794:799	a diffusible signal	781:799	the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar	725:811	We determined that changes in the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar were required for induction of the antimicrobial activity.					
36129764	4	61	from	changes	714:720	arg1	composition					739:749	the headspace composition	725:749	the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar	725:811	We determined that changes in the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar were required for induction of the antimicrobial activity.					
36129764	4	61	from	changes	714:720	arg1	volatiles					758:766	volatiles	758:766	volatiles	758:766	We determined that changes in the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar were required for induction of the antimicrobial activity.					
36129764	0	62	dep	Strength	0:7	arg1	Activity					70:77	Density-Dependent Volatile-Induced Antimicrobial Activity	21:77	Strength in Numbers: Density-Dependent Volatile-Induced Antimicrobial Activity by Xanthomonas perforans.	0:103	Strength in Numbers: Density-Dependent Volatile-Induced Antimicrobial Activity by Xanthomonas perforans.					
36129764	2	63	theme	X.	273:274	arg1	euvesicatoria					276:288	X. euvesicatoria	273:288	X. euvesicatoria	273:288	X. perforans quickly replaced X. euvesicatoria, mainly because of production of three bacteriocins (BCNs) against X. euvesicatoria; however, X. perforans outcompeted X. euvesicatoria even when the three known BCNs were deleted.					
36129764	1	64	from	spot	216:219	arg1	Florida					234:240	Florida	234:240	Florida	234:240	For most of the 20th century, Xanthomonas euvesicatoria was the only known bacterium associated with bacterial spot of tomato in Florida.					
36129764	9	65	from	metabolites	1805:1815	arg1	bacteria					1826:1833	other bacteria	1820:1833	other bacteria	1820:1833	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	66	theme	N-	1731:1732	arg1	lactone					1765:1771	N-(3-hydroxy-butanoyl)-homoserine lactone	1731:1771	N-(3-hydroxy-butanoyl)-homoserine lactone	1731:1771	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	66	theme	N-	1731:1732	arg1	metabolites					1805:1815	known quorum-sensing metabolites	1784:1815	known quorum-sensing metabolites in other bacteria	1784:1833	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	9	66	theme	N-	1731:1732	arg1	lactone					1719:1725	N-butanoyl-L-homoserine lactone	1695:1725	N-butanoyl-L-homoserine lactone	1695:1725	Among the compounds identified in the zone of inhibition were N-butanoyl-L-homoserine lactone and N-(3-hydroxy-butanoyl)-homoserine lactone, which are known quorum-sensing metabolites in other bacteria.					
36129764	5	67	theme	X.	1017:1018	arg1	perforans					1020:1028	X. perforans	1017:1028	X. perforans	1017:1028	Other Xanthomonas species also produced volatile-induced antimicrobial compounds against X. euvesicatoria and elicited antimicrobial activity by X. perforans.					
36129764	1	68	theme	Xanthomonas	135:145	arg1	bacterium					180:188	the only known bacterium	165:188	the only known bacterium associated with bacterial spot of tomato in Florida	165:240	For most of the 20th century, Xanthomonas euvesicatoria was the only known bacterium associated with bacterial spot of tomato in Florida.					
36129764	1	68	theme	Xanthomonas	135:145	arg1	euvesicatoria					147:159	Xanthomonas euvesicatoria	135:159	Xanthomonas euvesicatoria	135:159	For most of the 20th century, Xanthomonas euvesicatoria was the only known bacterium associated with bacterial spot of tomato in Florida.					
36129764	0	69	theme	Xanthomonas	82:92	arg1	perforans					94:102	Xanthomonas perforans	82:102	Xanthomonas perforans	82:102	Strength in Numbers: Density-Dependent Volatile-Induced Antimicrobial Activity by Xanthomonas perforans.					
36129764	3	70	theme	Petri	656:660	arg1	plates					662:667	Petri plates	656:667	Petri plates containing only one spot	656:692	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
36129764	8	71	theme	antimicrobial	1481:1493	arg1	present					1508:1514	present	1508:1514	present	1508:1514	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	8	71	theme	antimicrobial	1481:1493	arg1	activity					1495:1502	the antimicrobial activity	1477:1502	the antimicrobial activity	1477:1502	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	8	72	theme	antimicrobial	1301:1313	arg1	compounds					1315:1323	potential antimicrobial compounds	1291:1323	potential antimicrobial compounds	1291:1323	To identify potential antimicrobial compounds, we performed liquid chromatography with high-resolution mass spectrometry of the agar surrounding the spot in the high cell density Petri plates where the antimicrobial activity was present compared with agar surrounding the spot in Petri plates with one spot where antimicrobial activity was not observed.					
36129764	4	73	dep	volatiles	758:766	arg1	i.e.					752:755	i.e.	752:755	i.e.	752:755	We determined that changes in the headspace composition (i.e., volatiles) rather than a diffusible signal in the agar were required for induction of the antimicrobial activity.					
36129764	3	74	contain	containing	619:628	arg1	plates					612:617	Petri plates	606:617	Petri plates containing multiple spots	606:643	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
36129764	3	74	contain	containing	619:628	arg2	spots					639:643	multiple spots	630:643	multiple spots	630:643	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
36129764	2	75	dep	X.	384:385	arg1	perforans					387:395	X. perforans	384:395	X. perforans	384:395	X. perforans quickly replaced X. euvesicatoria, mainly because of production of three bacteriocins (BCNs) against X. euvesicatoria; however, X. perforans outcompeted X. euvesicatoria even when the three known BCNs were deleted.					
36129764	3	76	theme	X.	528:529	arg1	euvesicatoria					531:543	X. euvesicatoria	528:543	X. euvesicatoria	528:543	Surprisingly, we observed antimicrobial activity against X. euvesicatoria in the BCN triple mutant when the triple mutant was grown in Petri plates containing multiple spots but not in Petri plates containing only one spot.					
37159255	12	0	theme	tissue	2085:2090	arg1	engineering					2092:2102	cartilage tissue engineering	2075:2102	cartilage tissue engineering	2075:2102	In the CSMA/GelMA hydrogel, 6% (w/v) CSMA with an initial Young's modulus around 33 kPa was recommended for cartilage tissue engineering.					
37159255	8	1	from	protrusions	1232:1242	arg1	hydrogels					1252:1260	soft hydrogels	1247:1260	soft hydrogels	1247:1260	Afterwards, cellular protrusions in soft hydrogels remained filopodium-like from day 14 to day 28, while most protrusions were lamellipodium-like in stiff hydrogels on day 14 and then transformed into a spherical shape on day 28.					
37159255	1	2	theme	mesenchymal	250:260	arg1	MSCs					274:277	MSCs	274:277	MSCs	274:277	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	1	2	theme	mesenchymal	250:260	arg1	cells					267:271	mesenchymal stem cells	250:271	mesenchymal stem cells (MSCs)	250:278	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	9	3	theme	immunohistochemical	1511:1529	arg1	staining					1531:1538	immunohistochemical staining	1511:1538	immunohistochemical staining	1511:1538	The expression of chondrogenic markers analysed by real-time qPCR and immunohistochemical staining demonstrated that the optimal CS concentration for chondrogenesis was 6% (w/v) regardless of the stiffness of hydrogels.					
37159255	1	4	theme	cells	267:271	arg1	chondrogenesis					232:245	chondrogenesis	232:245	chondrogenesis of mesenchymal stem cells (MSCs)	232:278	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	6	5	theme	hydrogels	1033:1041	arg1	hydrogels					1033:1041	hydrogels	1033:1041	hydrogels	1033:1041	MSCs were encapsulated in the six groups of hydrogels and they underwent 28-day chondrogenic differentiation.					
37159255	6	5	theme	hydrogels	1033:1041	arg1	groups					1023:1028	the six groups	1015:1028	the six groups of hydrogels	1015:1041	MSCs were encapsulated in the six groups of hydrogels and they underwent 28-day chondrogenic differentiation.					
37159255	3	6	dep	%	564:564	arg1	w/v					567:569	w/v	567:569	w/v	567:569	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	4	7	theme	composition	736:746	arg1	hydrogels					718:726	The hydrogels	714:726	The hydrogels of each composition	714:746	The hydrogels of each composition were prepared with two stiffness values (33.36 ± 8.25 kPa vs. 8.42 ± 2.83 kPa).					
37159255	4	8	theme	stiffness	771:779	arg1	values					781:786	two stiffness values	767:786	two stiffness values (33.36 ± 8.25 kPa vs. 8.42 ± 2.83 kPa)	767:825	The hydrogels of each composition were prepared with two stiffness values (33.36 ± 8.25 kPa vs. 8.42 ± 2.83 kPa).					
37159255	12	9	theme	6	1995:1995	arg1	%					1996:1996	%	1996:1996	%	1996:1996	In the CSMA/GelMA hydrogel, 6% (w/v) CSMA with an initial Young's modulus around 33 kPa was recommended for cartilage tissue engineering.					
37159255	1	10	theme	cartilage	184:192	arg1	sulfate					140:146	Chondroitin sulfate	128:146	Chondroitin sulfate (CS)	128:151	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	1	10	theme	cartilage	184:192	arg1	glycosaminoglycan					156:172	a glycosaminoglycan	154:172	a glycosaminoglycan of native cartilage	154:192	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	11	11	theme	concentration	1906:1918	arg1	optimization					1885:1896	the optimization	1881:1896	the optimization of CSMA concentration	1881:1918	To summarize, this study presents an advancement in the optimization of CSMA concentration and stiffness of hydrogels for chondrogenesis.					
37159255	12	12	theme	Young	2025:2029	arg1	modulus					2033:2039	an initial Young's modulus	2014:2039	an initial Young's modulus around 33 kPa	2014:2053	In the CSMA/GelMA hydrogel, 6% (w/v) CSMA with an initial Young's modulus around 33 kPa was recommended for cartilage tissue engineering.					
37159255	3	13	theme	methacrylated	658:670	arg1	CSMA					693:696	CSMA	693:696	CSMA	693:696	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	3	13	theme	methacrylated	658:670	arg1	sulfate					684:690	methacrylated chondroitin sulfate	658:690	methacrylated chondroitin sulfate (CSMA)	658:697	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	8	14	from	hydrogels	1366:1374	arg1	day					1379:1381	day 14	1379:1384	day 14	1379:1384	Afterwards, cellular protrusions in soft hydrogels remained filopodium-like from day 14 to day 28, while most protrusions were lamellipodium-like in stiff hydrogels on day 14 and then transformed into a spherical shape on day 28.					
37159255	8	14	from	hydrogels	1366:1374	arg1	lamellipodium-like					1338:1355	lamellipodium-like	1338:1355	lamellipodium-like	1338:1355	Afterwards, cellular protrusions in soft hydrogels remained filopodium-like from day 14 to day 28, while most protrusions were lamellipodium-like in stiff hydrogels on day 14 and then transformed into a spherical shape on day 28.					
37159255	11	15	theme	hydrogels	1937:1945	arg1	stiffness					1924:1932	stiffness	1924:1932	stiffness of hydrogels for chondrogenesis	1924:1964	To summarize, this study presents an advancement in the optimization of CSMA concentration and stiffness of hydrogels for chondrogenesis.					
37159255	11	15	theme	hydrogels	1937:1945	arg1	advancement					1866:1876	an advancement	1863:1876	an advancement in the optimization of CSMA concentration	1863:1918	To summarize, this study presents an advancement in the optimization of CSMA concentration and stiffness of hydrogels for chondrogenesis.					
37159255	3	16	theme	%	564:564	arg1	GelMA					594:598	GelMA	594:598	GelMA	594:598	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	3	16	theme	%	564:564	arg1	methacryloyl					580:591	6% (w/v) gelatin methacryloyl	563:591	6% (w/v) gelatin methacryloyl (GelMA)	563:599	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	1	17	theme	Chondroitin	128:138	arg1	sulfate					140:146	Chondroitin sulfate	128:146	Chondroitin sulfate (CS)	128:151	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	1	17	theme	Chondroitin	128:138	arg1	glycosaminoglycan					156:172	a glycosaminoglycan	154:172	a glycosaminoglycan of native cartilage	154:192	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	1	17	theme	Chondroitin	128:138	arg1	CS					149:150	CS	149:150	CS	149:150	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	11	18	from	stiffness	1924:1932	arg1	optimization					1885:1896	the optimization	1881:1896	the optimization of CSMA concentration	1881:1918	To summarize, this study presents an advancement in the optimization of CSMA concentration and stiffness of hydrogels for chondrogenesis.					
37159255	5	19	theme	swelling	921:928	arg1	ratios					930:935	higher swelling ratios	914:935	higher swelling ratios	914:935	Physical characterization showed similar microporous structures among the six groups, higher swelling ratios and faster degradation in the soft hydrogel groups.					
37159255	10	20	theme	same	1683:1686	arg1	concentration					1693:1705	the same CSMA concentration	1679:1705	the same CSMA concentration	1679:1705	In addition, with the same CSMA concentration, the trend was observed that the stiff hydrogels supported superior chondrogenesis of MSCs compared to the soft hydrogel.					
37159255	2	21	theme	stiffness	473:481	arg1	effect					442:447	the effect	438:447	the effect of CS concentration and stiffness of CS-containing hydrogels on the chondrogenesis of MSCs	438:538	Herein, this study aimed at assessing the effect of CS concentration and stiffness of CS-containing hydrogels on the chondrogenesis of MSCs.					
37159255	7	22	from	viability	1108:1116	arg1	group					1126:1130	each group	1121:1130	each group	1121:1130	The cell viability in each group on day 1 was similar and most cells exhibited a round shape without spreading.					
37159255	7	22	from	viability	1108:1116	arg1	day					1135:1137	day 1	1135:1139	day 1	1135:1139	The cell viability in each group on day 1 was similar and most cells exhibited a round shape without spreading.					
37159255	6	23	theme	28-day	1062:1067	arg1	differentiation					1082:1096	28-day chondrogenic differentiation	1062:1096	28-day chondrogenic differentiation	1062:1096	MSCs were encapsulated in the six groups of hydrogels and they underwent 28-day chondrogenic differentiation.					
37159255	3	24	theme	sulfate	684:690	arg1	GelMA					594:598	GelMA	594:598	GelMA	594:598	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	3	24	theme	sulfate	684:690	arg1	methacryloyl					580:591	6% (w/v) gelatin methacryloyl	563:591	6% (w/v) gelatin methacryloyl (GelMA)	563:599	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	3	24	theme	sulfate	684:690	arg1	concentrations					611:624	three concentrations	605:624	three concentrations	605:624	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	0	25	theme	stiffness	59:67	arg1	effect					4:9	The effect	0:9	The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells	0:125	The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells.					
37159255	1	26	theme	matrix	303:308	arg1	stiffness					310:318	matrix stiffness	303:318	matrix stiffness in a CS-containing 3D environment	303:352	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	3	27	theme	gelatin	572:578	arg1	GelMA					594:598	GelMA	594:598	GelMA	594:598	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	3	27	theme	gelatin	572:578	arg1	methacryloyl					580:591	6% (w/v) gelatin methacryloyl	563:591	6% (w/v) gelatin methacryloyl (GelMA)	563:599	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	2	28	theme	concentration	455:467	arg1	effect					442:447	the effect	438:447	the effect of CS concentration and stiffness of CS-containing hydrogels on the chondrogenesis of MSCs	438:538	Herein, this study aimed at assessing the effect of CS concentration and stiffness of CS-containing hydrogels on the chondrogenesis of MSCs.					
37159255	8	29	theme	stiff	1360:1364	arg1	hydrogels					1366:1374	stiff hydrogels	1360:1374	stiff hydrogels on day 14	1360:1384	Afterwards, cellular protrusions in soft hydrogels remained filopodium-like from day 14 to day 28, while most protrusions were lamellipodium-like in stiff hydrogels on day 14 and then transformed into a spherical shape on day 28.					
37159255	0	30	theme	chondrogenic	72:83	arg1	differentiation					85:99	chondrogenic differentiation	72:99	chondrogenic differentiation of mesenchymal stem cells	72:125	The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells.					
37159255	4	31	theme	±	815:815	arg1	kPa					822:824	8.42 ± 2.83 kPa	810:824	8.42 ± 2.83 kPa	810:824	The hydrogels of each composition were prepared with two stiffness values (33.36 ± 8.25 kPa vs. 8.42 ± 2.83 kPa).					
37159255	8	32	from	lamellipodium-like	1338:1355	arg1	hydrogels					1366:1374	stiff hydrogels	1360:1374	stiff hydrogels on day 14	1360:1384	Afterwards, cellular protrusions in soft hydrogels remained filopodium-like from day 14 to day 28, while most protrusions were lamellipodium-like in stiff hydrogels on day 14 and then transformed into a spherical shape on day 28.					
37159255	1	33	theme	CS-containing	325:337	arg1	environment					342:352	a CS-containing 3D environment	323:352	a CS-containing 3D environment	323:352	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	2	34	theme	CS-containing	486:498	arg1	hydrogels					500:508	CS-containing hydrogels	486:508	CS-containing hydrogels	486:508	Herein, this study aimed at assessing the effect of CS concentration and stiffness of CS-containing hydrogels on the chondrogenesis of MSCs.					
37159255	0	35	theme	stem	116:119	arg1	cells					121:125	mesenchymal stem cells	104:125	mesenchymal stem cells	104:125	The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells.					
37159255	5	36	theme	hydrogel	972:979	arg1	groups					981:986	the soft hydrogel groups	963:986	the soft hydrogel groups	963:986	Physical characterization showed similar microporous structures among the six groups, higher swelling ratios and faster degradation in the soft hydrogel groups.					
37159255	12	37	dep	%	1996:1996	arg1	w/v					1999:2001	w/v	1999:2001	w/v	1999:2001	In the CSMA/GelMA hydrogel, 6% (w/v) CSMA with an initial Young's modulus around 33 kPa was recommended for cartilage tissue engineering.					
37159255	2	38	from	effect	442:447	arg1	chondrogenesis					517:530	the chondrogenesis	513:530	the chondrogenesis of MSCs	513:538	Herein, this study aimed at assessing the effect of CS concentration and stiffness of CS-containing hydrogels on the chondrogenesis of MSCs.					
37159255	12	39	with	CSMA	2004:2007	arg1	modulus					2033:2039	an initial Young's modulus	2014:2039	an initial Young's modulus around 33 kPa	2014:2053	In the CSMA/GelMA hydrogel, 6% (w/v) CSMA with an initial Young's modulus around 33 kPa was recommended for cartilage tissue engineering.					
37159255	7	40	theme	round	1180:1184	arg1	shape					1186:1190	a round shape	1178:1190	a round shape	1178:1190	The cell viability in each group on day 1 was similar and most cells exhibited a round shape without spreading.					
37159255	8	41	theme	soft	1247:1250	arg1	hydrogels					1252:1260	soft hydrogels	1247:1260	soft hydrogels	1247:1260	Afterwards, cellular protrusions in soft hydrogels remained filopodium-like from day 14 to day 28, while most protrusions were lamellipodium-like in stiff hydrogels on day 14 and then transformed into a spherical shape on day 28.					
37159255	8	42	theme	spherical	1414:1422	arg1	shape					1424:1428	a spherical shape	1412:1428	a spherical shape on day 28	1412:1438	Afterwards, cellular protrusions in soft hydrogels remained filopodium-like from day 14 to day 28, while most protrusions were lamellipodium-like in stiff hydrogels on day 14 and then transformed into a spherical shape on day 28.					
37159255	0	43	theme	sulfate	26:32	arg1	concentration					34:46	chondroitin sulfate concentration	14:46	chondroitin sulfate concentration	14:46	The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells.					
37159255	7	44	theme	most	1157:1160	arg1	cells					1162:1166	most cells	1157:1166	most cells	1157:1166	The cell viability in each group on day 1 was similar and most cells exhibited a round shape without spreading.					
37159255	12	45	theme	initial	2017:2023	arg1	modulus					2033:2039	an initial Young's modulus	2014:2039	an initial Young's modulus around 33 kPa	2014:2053	In the CSMA/GelMA hydrogel, 6% (w/v) CSMA with an initial Young's modulus around 33 kPa was recommended for cartilage tissue engineering.					
37159255	1	46	from	stiffness	310:318	arg1	environment					342:352	a CS-containing 3D environment	323:352	a CS-containing 3D environment	323:352	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	9	47	theme	chondrogenic	1459:1470	arg1	markers					1472:1478	chondrogenic markers	1459:1478	chondrogenic markers analysed by real-time qPCR	1459:1505	The expression of chondrogenic markers analysed by real-time qPCR and immunohistochemical staining demonstrated that the optimal CS concentration for chondrogenesis was 6% (w/v) regardless of the stiffness of hydrogels.					
37159255	11	48	from	advancement	1866:1876	arg1	optimization					1885:1896	the optimization	1881:1896	the optimization of CSMA concentration	1881:1918	To summarize, this study presents an advancement in the optimization of CSMA concentration and stiffness of hydrogels for chondrogenesis.					
37159255	1	49	dep	shown	199:203	arg1	whereas					281:287	whereas	281:287	whereas	281:287	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	5	50	theme	similar	861:867	arg1	structures					881:890	similar microporous structures	861:890	similar microporous structures among the six groups	861:911	Physical characterization showed similar microporous structures among the six groups, higher swelling ratios and faster degradation in the soft hydrogel groups.					
37159255	10	51	located	observed	1722:1729	arg1	addition					1664:1671	addition	1664:1671	addition	1664:1671	In addition, with the same CSMA concentration, the trend was observed that the stiff hydrogels supported superior chondrogenesis of MSCs compared to the soft hydrogel.					
37159255	10	51	located	observed	1722:1729	arg2	trend					1712:1716	the trend	1708:1716	the trend	1708:1716	In addition, with the same CSMA concentration, the trend was observed that the stiff hydrogels supported superior chondrogenesis of MSCs compared to the soft hydrogel.					
37159255	9	52	theme	real-time	1492:1500	arg1	qPCR					1502:1505	real-time qPCR	1492:1505	real-time qPCR	1492:1505	The expression of chondrogenic markers analysed by real-time qPCR and immunohistochemical staining demonstrated that the optimal CS concentration for chondrogenesis was 6% (w/v) regardless of the stiffness of hydrogels.					
37159255	1	53	from	effect	293:298	arg1	chondrogenesis					357:370	chondrogenesis	357:370	chondrogenesis	357:370	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	1	53	from	effect	293:298	arg1	environment					342:352	a CS-containing 3D environment	323:352	a CS-containing 3D environment	323:352	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	10	54	theme	stiff	1740:1744	arg1	hydrogels					1746:1754	the stiff hydrogels	1736:1754	the stiff hydrogels	1736:1754	In addition, with the same CSMA concentration, the trend was observed that the stiff hydrogels supported superior chondrogenesis of MSCs compared to the soft hydrogel.					
37159255	1	55	theme	stem	262:265	arg1	MSCs					274:277	MSCs	274:277	MSCs	274:277	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	1	55	theme	stem	262:265	arg1	cells					267:271	mesenchymal stem cells	250:271	mesenchymal stem cells (MSCs)	250:278	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	10	56	theme	MSCs	1793:1796	arg1	chondrogenesis					1775:1788	superior chondrogenesis	1766:1788	superior chondrogenesis of MSCs	1766:1796	In addition, with the same CSMA concentration, the trend was observed that the stiff hydrogels supported superior chondrogenesis of MSCs compared to the soft hydrogel.					
37159255	12	57	theme	CSMA/GelMA	1974:1983	arg1	hydrogel					1985:1992	the CSMA/GelMA hydrogel	1970:1992	the CSMA/GelMA hydrogel	1970:1992	In the CSMA/GelMA hydrogel, 6% (w/v) CSMA with an initial Young's modulus around 33 kPa was recommended for cartilage tissue engineering.					
37159255	1	58	theme	native	177:182	arg1	cartilage					184:192	native cartilage	177:192	native cartilage	177:192	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	9	59	theme	optimal	1562:1568	arg1	%					1611:1611	6%	1610:1611	6% (w/v)	1610:1617	The expression of chondrogenic markers analysed by real-time qPCR and immunohistochemical staining demonstrated that the optimal CS concentration for chondrogenesis was 6% (w/v) regardless of the stiffness of hydrogels.					
37159255	9	59	theme	optimal	1562:1568	arg1	concentration					1573:1585	the optimal CS concentration	1558:1585	the optimal CS concentration for chondrogenesis	1558:1604	The expression of chondrogenic markers analysed by real-time qPCR and immunohistochemical staining demonstrated that the optimal CS concentration for chondrogenesis was 6% (w/v) regardless of the stiffness of hydrogels.					
37159255	10	60	theme	soft	1814:1817	arg1	hydrogel					1819:1826	the soft hydrogel	1810:1826	the soft hydrogel	1810:1826	In addition, with the same CSMA concentration, the trend was observed that the stiff hydrogels supported superior chondrogenesis of MSCs compared to the soft hydrogel.					
37159255	4	61	theme	±	795:795	arg1	kPa					802:804	33.36 ± 8.25 kPa	789:804	33.36 ± 8.25 kPa	789:804	The hydrogels of each composition were prepared with two stiffness values (33.36 ± 8.25 kPa vs. 8.42 ± 2.83 kPa).					
37159255	5	62	theme	faster	941:946	arg1	degradation					948:958	faster degradation	941:958	faster degradation	941:958	Physical characterization showed similar microporous structures among the six groups, higher swelling ratios and faster degradation in the soft hydrogel groups.					
37159255	8	63	from	day	1292:1294	arg1	filopodium-like					1271:1285	filopodium-like	1271:1285	filopodium-like	1271:1285	Afterwards, cellular protrusions in soft hydrogels remained filopodium-like from day 14 to day 28, while most protrusions were lamellipodium-like in stiff hydrogels on day 14 and then transformed into a spherical shape on day 28.					
37159255	11	64	dep	summarize	1832:1840	arg1	To					1829:1830	To	1829:1830	To	1829:1830	To summarize, this study presents an advancement in the optimization of CSMA concentration and stiffness of hydrogels for chondrogenesis.					
37159255	11	65	theme	CSMA	1901:1904	arg1	concentration					1906:1918	CSMA concentration	1901:1918	CSMA concentration	1901:1918	To summarize, this study presents an advancement in the optimization of CSMA concentration and stiffness of hydrogels for chondrogenesis.					
37159255	8	66	theme	most	1316:1319	arg1	protrusions					1321:1331	most protrusions	1316:1331	most protrusions	1316:1331	Afterwards, cellular protrusions in soft hydrogels remained filopodium-like from day 14 to day 28, while most protrusions were lamellipodium-like in stiff hydrogels on day 14 and then transformed into a spherical shape on day 28.					
37159255	3	67	theme	chondroitin	672:682	arg1	CSMA					693:696	CSMA	693:696	CSMA	693:696	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	3	67	theme	chondroitin	672:682	arg1	sulfate					684:690	methacrylated chondroitin sulfate	658:690	methacrylated chondroitin sulfate (CSMA)	658:697	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	3	68	theme	6	563:563	arg1	%					564:564	%	564:564	%	564:564	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	2	69	theme	MSCs	535:538	arg1	chondrogenesis					517:530	the chondrogenesis	513:530	the chondrogenesis of MSCs	513:538	Herein, this study aimed at assessing the effect of CS concentration and stiffness of CS-containing hydrogels on the chondrogenesis of MSCs.					
37159255	5	70	theme	higher	914:919	arg1	ratios					930:935	higher swelling ratios	914:935	higher swelling ratios	914:935	Physical characterization showed similar microporous structures among the six groups, higher swelling ratios and faster degradation in the soft hydrogel groups.					
37159255	10	71	theme	CSMA	1688:1691	arg1	concentration					1693:1705	the same CSMA concentration	1679:1705	the same CSMA concentration	1679:1705	In addition, with the same CSMA concentration, the trend was observed that the stiff hydrogels supported superior chondrogenesis of MSCs compared to the soft hydrogel.					
37159255	6	72	theme	chondrogenic	1069:1080	arg1	differentiation					1082:1096	28-day chondrogenic differentiation	1062:1096	28-day chondrogenic differentiation	1062:1096	MSCs were encapsulated in the six groups of hydrogels and they underwent 28-day chondrogenic differentiation.					
37159255	7	73	theme	cell	1103:1106	arg1	similar					1145:1151	similar	1145:1151	similar	1145:1151	The cell viability in each group on day 1 was similar and most cells exhibited a round shape without spreading.					
37159255	7	73	theme	cell	1103:1106	arg1	viability					1108:1116	The cell viability	1099:1116	The cell viability in each group on day 1	1099:1139	The cell viability in each group on day 1 was similar and most cells exhibited a round shape without spreading.					
37159255	4	74	dep	values	781:786	arg1	kPa					822:824	8.42 ± 2.83 kPa	810:824	8.42 ± 2.83 kPa	810:824	The hydrogels of each composition were prepared with two stiffness values (33.36 ± 8.25 kPa vs. 8.42 ± 2.83 kPa).					
37159255	4	74	dep	values	781:786	arg1	kPa					802:804	33.36 ± 8.25 kPa	789:804	33.36 ± 8.25 kPa	789:804	The hydrogels of each composition were prepared with two stiffness values (33.36 ± 8.25 kPa vs. 8.42 ± 2.83 kPa).					
37159255	1	75	theme	stiffness	310:318	arg1	effect					293:298	the effect	289:298	the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis	289:370	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	2	76	theme	CS	452:453	arg1	concentration					455:467	CS concentration	452:467	CS concentration	452:467	Herein, this study aimed at assessing the effect of CS concentration and stiffness of CS-containing hydrogels on the chondrogenesis of MSCs.					
37159255	0	77	from	effect	4:9	arg1	differentiation					85:99	chondrogenic differentiation	72:99	chondrogenic differentiation of mesenchymal stem cells	72:125	The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells.					
37159255	0	78	theme	mesenchymal	104:114	arg1	cells					121:125	mesenchymal stem cells	104:125	mesenchymal stem cells	104:125	The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells.					
37159255	2	79	theme	hydrogels	500:508	arg1	stiffness					473:481	stiffness	473:481	stiffness of CS-containing hydrogels	473:508	Herein, this study aimed at assessing the effect of CS concentration and stiffness of CS-containing hydrogels on the chondrogenesis of MSCs.					
37159255	2	79	theme	hydrogels	500:508	arg1	concentration					455:467	CS concentration	452:467	CS concentration	452:467	Herein, this study aimed at assessing the effect of CS concentration and stiffness of CS-containing hydrogels on the chondrogenesis of MSCs.					
37159255	12	80	theme	cartilage	2075:2083	arg1	engineering					2092:2102	cartilage tissue engineering	2075:2102	cartilage tissue engineering	2075:2102	In the CSMA/GelMA hydrogel, 6% (w/v) CSMA with an initial Young's modulus around 33 kPa was recommended for cartilage tissue engineering.					
37159255	0	81	theme	cells	121:125	arg1	differentiation					85:99	chondrogenic differentiation	72:99	chondrogenic differentiation of mesenchymal stem cells	72:125	The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells.					
37159255	1	82	theme	3D	339:340	arg1	environment					342:352	a CS-containing 3D environment	323:352	a CS-containing 3D environment	323:352	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	5	83	theme	Physical	828:835	arg1	characterization					837:852	Physical characterization	828:852	Physical characterization	828:852	Physical characterization showed similar microporous structures among the six groups, higher swelling ratios and faster degradation in the soft hydrogel groups.					
37159255	9	84	theme	hydrogels	1650:1658	arg1	stiffness					1637:1645	the stiffness	1633:1645	the stiffness of hydrogels	1633:1658	The expression of chondrogenic markers analysed by real-time qPCR and immunohistochemical staining demonstrated that the optimal CS concentration for chondrogenesis was 6% (w/v) regardless of the stiffness of hydrogels.					
37159255	1	85	from	environment	342:352	arg1	effect					293:298	the effect	289:298	the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis	289:370	Chondroitin sulfate (CS), a glycosaminoglycan of native cartilage, has shown its potential in promoting chondrogenesis of mesenchymal stem cells (MSCs), whereas the effect of matrix stiffness in a CS-containing 3D environment on chondrogenesis is still poorly understood.					
37159255	5	86	theme	soft	967:970	arg1	groups					981:986	the soft hydrogel groups	963:986	the soft hydrogel groups	963:986	Physical characterization showed similar microporous structures among the six groups, higher swelling ratios and faster degradation in the soft hydrogel groups.					
37159255	8	87	theme	cellular	1223:1230	arg1	protrusions					1232:1242	cellular protrusions	1223:1242	cellular protrusions in soft hydrogels	1223:1260	Afterwards, cellular protrusions in soft hydrogels remained filopodium-like from day 14 to day 28, while most protrusions were lamellipodium-like in stiff hydrogels on day 14 and then transformed into a spherical shape on day 28.					
37159255	0	88	theme	chondroitin	14:24	arg1	sulfate					26:32	chondroitin sulfate	14:32	chondroitin sulfate concentration	14:46	The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells.					
37159255	3	89	dep	%	634:634	arg1	i.e.					627:630	i.e.	627:630	i.e.	627:630	Hydrogels composed of 6% (w/v) gelatin methacryloyl (GelMA) and three concentrations, i.e., 4%, 6%, or 10% (w/v), of methacrylated chondroitin sulfate (CSMA) were prepared.					
37159255	0	90	theme	concentration	34:46	arg1	effect					4:9	The effect	0:9	The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells	0:125	The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells.					
37159255	8	91	from	shape	1424:1428	arg1	day					1433:1435	day 28	1433:1438	day 28	1433:1438	Afterwards, cellular protrusions in soft hydrogels remained filopodium-like from day 14 to day 28, while most protrusions were lamellipodium-like in stiff hydrogels on day 14 and then transformed into a spherical shape on day 28.					
37159255	0	92	theme	matrix	52:57	arg1	stiffness					59:67	matrix stiffness	52:67	matrix stiffness	52:67	The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells.					
37159255	9	93	theme	CS	1570:1571	arg1	%					1611:1611	6%	1610:1611	6% (w/v)	1610:1617	The expression of chondrogenic markers analysed by real-time qPCR and immunohistochemical staining demonstrated that the optimal CS concentration for chondrogenesis was 6% (w/v) regardless of the stiffness of hydrogels.					
37159255	9	93	theme	CS	1570:1571	arg1	concentration					1573:1585	the optimal CS concentration	1558:1585	the optimal CS concentration for chondrogenesis	1558:1604	The expression of chondrogenic markers analysed by real-time qPCR and immunohistochemical staining demonstrated that the optimal CS concentration for chondrogenesis was 6% (w/v) regardless of the stiffness of hydrogels.					
37159255	9	94	theme	markers	1472:1478	arg1	staining					1531:1538	immunohistochemical staining	1511:1538	immunohistochemical staining	1511:1538	The expression of chondrogenic markers analysed by real-time qPCR and immunohistochemical staining demonstrated that the optimal CS concentration for chondrogenesis was 6% (w/v) regardless of the stiffness of hydrogels.					
37159255	9	94	theme	markers	1472:1478	arg1	expression					1445:1454	The expression	1441:1454	The expression of chondrogenic markers analysed by real-time qPCR	1441:1505	The expression of chondrogenic markers analysed by real-time qPCR and immunohistochemical staining demonstrated that the optimal CS concentration for chondrogenesis was 6% (w/v) regardless of the stiffness of hydrogels.					
37159255	12	95	theme	%	1996:1996	arg1	CSMA					2004:2007	6% (w/v) CSMA	1995:2007	6% (w/v) CSMA with an initial Young's modulus around 33 kPa	1995:2053	In the CSMA/GelMA hydrogel, 6% (w/v) CSMA with an initial Young's modulus around 33 kPa was recommended for cartilage tissue engineering.					
37159255	5	96	theme	microporous	869:879	arg1	structures					881:890	similar microporous structures	861:890	similar microporous structures among the six groups	861:911	Physical characterization showed similar microporous structures among the six groups, higher swelling ratios and faster degradation in the soft hydrogel groups.					
37159255	10	97	theme	superior	1766:1773	arg1	chondrogenesis					1775:1788	superior chondrogenesis	1766:1788	superior chondrogenesis of MSCs	1766:1796	In addition, with the same CSMA concentration, the trend was observed that the stiff hydrogels supported superior chondrogenesis of MSCs compared to the soft hydrogel.					
35450633	5	0	theme	paper-making	1251:1262	arg1	industry					1264:1271	the paper-making industry	1247:1271	the paper-making industry	1247:1271	Overall, the feasibility of large-scale production of biodegradable packaging materials in the paper-making industry is demonstrated by the fact that the micro/nanostructures and hydrophobic surfaces could be directly constructed on cellulosic paperboard.					
35450633	3	1	theme	remarkable	745:754	arg1	strength					768:775	remarkable wet tensile strength	745:775	remarkable wet tensile strength	745:775	The as-obtained multilayered papers demonstrated impressive dry tensile strength and remarkable wet tensile strength of 6542.5 N/m and 5875 N/m, which were increased by 56% and 2277%, respectively.					
35450633	2	2	theme	composite	620:628	arg1	papers					630:635	this biodegradable composite papers	601:635	this biodegradable composite papers	601:635	Owning to the laminated process followed by spraying approach, MFC and n-SiO2 were assembled onto two surfaces of the cellulose paper base, respectively, endowing the mechanical behaviors and superhydrophobic performance of this biodegradable composite papers as packaging material.					
35450633	5	3	theme	materials	1234:1242	arg1	production					1196:1205	large-scale production	1184:1205	large-scale production of biodegradable packaging materials	1184:1242	Overall, the feasibility of large-scale production of biodegradable packaging materials in the paper-making industry is demonstrated by the fact that the micro/nanostructures and hydrophobic surfaces could be directly constructed on cellulosic paperboard.					
35450633	0	4	theme	micro-fibrillated	88:104	arg1	cellulose					106:114	micro-fibrillated cellulose	88:114	micro-fibrillated cellulose	88:114	High-strength and super-hydrophobic multilayered paper based on nano-silica coating and micro-fibrillated cellulose.					
35450633	3	5	theme	tensile	724:730	arg1	strength					732:739	impressive dry tensile strength	709:739	impressive dry tensile strength	709:739	The as-obtained multilayered papers demonstrated impressive dry tensile strength and remarkable wet tensile strength of 6542.5 N/m and 5875 N/m, which were increased by 56% and 2277%, respectively.					
35450633	1	6	with	high-strength	172:184	arg1	properties					263:272	improved moisture and air barrier properties	229:272	properties	263:272	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	1	6	with	high-strength	172:184	arg1	moisture					238:245	improved moisture and air barrier properties	229:272	moisture	238:245	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	4	7	theme	rational	899:906	arg1	properties					919:928	rational mechanical properties	899:928	rational mechanical properties	899:928	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	5	8	from	feasibility	1169:1179	arg1	industry					1264:1271	the paper-making industry	1247:1271	the paper-making industry	1247:1271	Overall, the feasibility of large-scale production of biodegradable packaging materials in the paper-making industry is demonstrated by the fact that the micro/nanostructures and hydrophobic surfaces could be directly constructed on cellulosic paperboard.					
35450633	1	9	with	cellulose	334:342	arg1	properties					263:272	improved moisture and air barrier properties	229:272	properties	263:272	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	1	9	with	cellulose	334:342	arg1	moisture					238:245	improved moisture and air barrier properties	229:272	moisture	238:245	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	4	10	contain	possessed	930:938	arg1	paper					888:892	the multilayered paper	871:892	the multilayered paper with rational mechanical properties	871:928	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	4	10	contain	possessed	930:938	arg2	performance					1109:1119	a superhydrophobic performance	1090:1119	a superhydrophobic performance with the contact angle of ~151.2°	1090:1153	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	4	10	contain	possessed	930:938	arg2	permeabilities					944:957	low permeabilities	940:957	low permeabilities	940:957	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	4	11	with	permeabilities	944:957	arg1	angle					1138:1142	the contact angle	1126:1142	the contact angle of ~151.2°	1126:1153	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	2	12	theme	base	511:514	arg1	surfaces					479:486	two surfaces	475:486	two surfaces of the cellulose paper base	475:514	Owning to the laminated process followed by spraying approach, MFC and n-SiO2 were assembled onto two surfaces of the cellulose paper base, respectively, endowing the mechanical behaviors and superhydrophobic performance of this biodegradable composite papers as packaging material.					
35450633	4	13	theme	~151.2°	1147:1153	arg1	angle					1138:1142	the contact angle	1126:1142	the contact angle of ~151.2°	1126:1153	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	2	14	theme	paper	505:509	arg1	base					511:514	the cellulose paper base	491:514	the cellulose paper base	491:514	Owning to the laminated process followed by spraying approach, MFC and n-SiO2 were assembled onto two surfaces of the cellulose paper base, respectively, endowing the mechanical behaviors and superhydrophobic performance of this biodegradable composite papers as packaging material.					
35450633	3	15	theme	wet	756:758	arg1	strength					768:775	remarkable wet tensile strength	745:775	remarkable wet tensile strength	745:775	The as-obtained multilayered papers demonstrated impressive dry tensile strength and remarkable wet tensile strength of 6542.5 N/m and 5875 N/m, which were increased by 56% and 2277%, respectively.					
35450633	2	16	theme	packaging	640:648	arg1	material					650:657	packaging material	640:657	packaging material	640:657	Owning to the laminated process followed by spraying approach, MFC and n-SiO2 were assembled onto two surfaces of the cellulose paper base, respectively, endowing the mechanical behaviors and superhydrophobic performance of this biodegradable composite papers as packaging material.					
35450633	1	17	theme	super-hydrophobic	190:206	arg1	paper					218:222	super-hydrophobic packaging paper	190:222	super-hydrophobic packaging paper	190:222	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	3	18	theme	tensile	760:766	arg1	strength					768:775	remarkable wet tensile strength	745:775	remarkable wet tensile strength	745:775	The as-obtained multilayered papers demonstrated impressive dry tensile strength and remarkable wet tensile strength of 6542.5 N/m and 5875 N/m, which were increased by 56% and 2277%, respectively.					
35450633	5	19	theme	biodegradable	1210:1222	arg1	materials					1234:1242	biodegradable packaging materials	1210:1242	biodegradable packaging materials	1210:1242	Overall, the feasibility of large-scale production of biodegradable packaging materials in the paper-making industry is demonstrated by the fact that the micro/nanostructures and hydrophobic surfaces could be directly constructed on cellulosic paperboard.					
35450633	3	20	theme	5875 N/m	795:802	arg1	strength					768:775	remarkable wet tensile strength	745:775	remarkable wet tensile strength	745:775	The as-obtained multilayered papers demonstrated impressive dry tensile strength and remarkable wet tensile strength of 6542.5 N/m and 5875 N/m, which were increased by 56% and 2277%, respectively.					
35450633	3	20	theme	5875 N/m	795:802	arg1	strength					732:739	impressive dry tensile strength	709:739	impressive dry tensile strength	709:739	The as-obtained multilayered papers demonstrated impressive dry tensile strength and remarkable wet tensile strength of 6542.5 N/m and 5875 N/m, which were increased by 56% and 2277%, respectively.					
35450633	2	21	theme	superhydrophobic	569:584	arg1	performance					586:596	superhydrophobic performance	569:596	superhydrophobic performance	569:596	Owning to the laminated process followed by spraying approach, MFC and n-SiO2 were assembled onto two surfaces of the cellulose paper base, respectively, endowing the mechanical behaviors and superhydrophobic performance of this biodegradable composite papers as packaging material.					
35450633	1	22	theme	packaging	208:216	arg1	paper					218:222	super-hydrophobic packaging paper	190:222	super-hydrophobic packaging paper	190:222	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	5	23	theme	production	1196:1205	arg1	feasibility					1169:1179	the feasibility	1165:1179	the feasibility of large-scale production of biodegradable packaging materials in the paper-making industry	1165:1271	Overall, the feasibility of large-scale production of biodegradable packaging materials in the paper-making industry is demonstrated by the fact that the micro/nanostructures and hydrophobic surfaces could be directly constructed on cellulosic paperboard.					
35450633	1	24	theme	cellulosic	298:307	arg1	pulps					309:313	cellulosic pulps	298:313	cellulosic pulps	298:313	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	2	25	theme	papers	630:635	arg1	behaviors					555:563	mechanical behaviors	544:563	mechanical behaviors	544:563	Owning to the laminated process followed by spraying approach, MFC and n-SiO2 were assembled onto two surfaces of the cellulose paper base, respectively, endowing the mechanical behaviors and superhydrophobic performance of this biodegradable composite papers as packaging material.					
35450633	2	25	theme	papers	630:635	arg1	performance					586:596	superhydrophobic performance	569:596	superhydrophobic performance	569:596	Owning to the laminated process followed by spraying approach, MFC and n-SiO2 were assembled onto two surfaces of the cellulose paper base, respectively, endowing the mechanical behaviors and superhydrophobic performance of this biodegradable composite papers as packaging material.					
35450633	4	26	theme	contact	1130:1136	arg1	angle					1138:1142	the contact angle	1126:1142	the contact angle of ~151.2°	1126:1153	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	2	27	theme	biodegradable	606:618	arg1	papers					630:635	this biodegradable composite papers	601:635	this biodegradable composite papers	601:635	Owning to the laminated process followed by spraying approach, MFC and n-SiO2 were assembled onto two surfaces of the cellulose paper base, respectively, endowing the mechanical behaviors and superhydrophobic performance of this biodegradable composite papers as packaging material.					
35450633	0	28	theme	multilayered	36:47	arg1	paper					49:53	super-hydrophobic multilayered paper	18:53	super-hydrophobic multilayered paper	18:53	High-strength and super-hydrophobic multilayered paper based on nano-silica coating and micro-fibrillated cellulose.					
35450633	2	29	theme	mechanical	544:553	arg1	behaviors					555:563	mechanical behaviors	544:563	mechanical behaviors	544:563	Owning to the laminated process followed by spraying approach, MFC and n-SiO2 were assembled onto two surfaces of the cellulose paper base, respectively, endowing the mechanical behaviors and superhydrophobic performance of this biodegradable composite papers as packaging material.					
35450633	4	30	theme	multilayered	875:886	arg1	paper					888:892	the multilayered paper	871:892	the multilayered paper with rational mechanical properties	871:928	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	0	31	theme	super-hydrophobic	18:34	arg1	paper					49:53	super-hydrophobic multilayered paper	18:53	super-hydrophobic multilayered paper	18:53	High-strength and super-hydrophobic multilayered paper based on nano-silica coating and micro-fibrillated cellulose.					
35450633	3	32	theme	6542.5 N/m	780:789	arg1	strength					768:775	remarkable wet tensile strength	745:775	remarkable wet tensile strength	745:775	The as-obtained multilayered papers demonstrated impressive dry tensile strength and remarkable wet tensile strength of 6542.5 N/m and 5875 N/m, which were increased by 56% and 2277%, respectively.					
35450633	3	32	theme	6542.5 N/m	780:789	arg1	strength					732:739	impressive dry tensile strength	709:739	impressive dry tensile strength	709:739	The as-obtained multilayered papers demonstrated impressive dry tensile strength and remarkable wet tensile strength of 6542.5 N/m and 5875 N/m, which were increased by 56% and 2277%, respectively.					
35450633	5	33	theme	hydrophobic	1335:1345	arg1	surfaces					1347:1354	hydrophobic surfaces	1335:1354	hydrophobic surfaces	1335:1354	Overall, the feasibility of large-scale production of biodegradable packaging materials in the paper-making industry is demonstrated by the fact that the micro/nanostructures and hydrophobic surfaces could be directly constructed on cellulosic paperboard.					
35450633	3	34	theme	as-obtained	664:674	arg1	papers					689:694	The as-obtained multilayered papers	660:694	The as-obtained multilayered papers	660:694	The as-obtained multilayered papers demonstrated impressive dry tensile strength and remarkable wet tensile strength of 6542.5 N/m and 5875 N/m, which were increased by 56% and 2277%, respectively.					
35450633	1	35	theme	improved	229:236	arg1	moisture					238:245	improved moisture and air barrier properties	229:272	moisture	238:245	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	4	36	theme	water	1031:1035	arg1	vapor					1037:1041	water vapor	1031:1041	water vapor (378.24 g·m-2·day-1)	1031:1062	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	4	36	theme	water	1031:1035	arg1	378.24 g·m-2·day-1					1044:1061	378.24 g·m-2·day-1	1044:1061	378.24 g·m-2·day-1	1044:1061	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	1	37	theme	micro-fibrillated	316:332	arg1	MFC					345:347	MFC	345:347	MFC	345:347	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	1	37	theme	micro-fibrillated	316:332	arg1	cellulose					334:342	micro-fibrillated cellulose	316:342	micro-fibrillated cellulose (MFC)	316:348	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	4	38	with	performance	1109:1119	arg1	angle					1138:1142	the contact angle	1126:1142	the contact angle of ~151.2°	1126:1153	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	3	39	theme	multilayered	676:687	arg1	papers					689:694	The as-obtained multilayered papers	660:694	The as-obtained multilayered papers	660:694	The as-obtained multilayered papers demonstrated impressive dry tensile strength and remarkable wet tensile strength of 6542.5 N/m and 5875 N/m, which were increased by 56% and 2277%, respectively.					
35450633	4	40	theme	superhydrophobic	1092:1107	arg1	performance					1109:1119	a superhydrophobic performance	1090:1119	a superhydrophobic performance with the contact angle of ~151.2°	1090:1153	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	4	41	theme	air	962:964	arg1	performance					1109:1119	a superhydrophobic performance	1090:1119	a superhydrophobic performance with the contact angle of ~151.2°	1090:1153	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	4	41	theme	air	962:964	arg1	permeabilities					944:957	low permeabilities	940:957	low permeabilities	940:957	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	5	42	theme	packaging	1224:1232	arg1	materials					1234:1242	biodegradable packaging materials	1210:1242	biodegradable packaging materials	1210:1242	Overall, the feasibility of large-scale production of biodegradable packaging materials in the paper-making industry is demonstrated by the fact that the micro/nanostructures and hydrophobic surfaces could be directly constructed on cellulosic paperboard.					
35450633	0	43	theme	nano-silica	64:74	arg1	coating					76:82	nano-silica coating	64:82	nano-silica coating	64:82	High-strength and super-hydrophobic multilayered paper based on nano-silica coating and micro-fibrillated cellulose.					
35450633	1	44	theme	facile	127:132	arg1	strategy					134:141	a facile strategy	125:141	a facile strategy	125:141	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	4	45	theme	vapor	1037:1041	arg1	performance					1109:1119	a superhydrophobic performance	1090:1119	a superhydrophobic performance with the contact angle of ~151.2°	1090:1153	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	4	45	theme	vapor	1037:1041	arg1	permeabilities					944:957	low permeabilities	940:957	low permeabilities	940:957	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	1	46	with	paper	218:222	arg1	properties					263:272	improved moisture and air barrier properties	229:272	properties	263:272	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	1	46	with	paper	218:222	arg1	moisture					238:245	improved moisture and air barrier properties	229:272	moisture	238:245	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	3	47	theme	impressive	709:718	arg1	strength					732:739	impressive dry tensile strength	709:739	impressive dry tensile strength	709:739	The as-obtained multilayered papers demonstrated impressive dry tensile strength and remarkable wet tensile strength of 6542.5 N/m and 5875 N/m, which were increased by 56% and 2277%, respectively.					
35450633	4	48	theme	oxygen	993:998	arg1	performance					1109:1119	a superhydrophobic performance	1090:1119	a superhydrophobic performance with the contact angle of ~151.2°	1090:1153	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	4	48	theme	oxygen	993:998	arg1	permeabilities					944:957	low permeabilities	940:957	low permeabilities	940:957	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	5	49	theme	large-scale	1184:1194	arg1	production					1196:1205	large-scale production	1184:1205	large-scale production of biodegradable packaging materials	1184:1242	Overall, the feasibility of large-scale production of biodegradable packaging materials in the paper-making industry is demonstrated by the fact that the micro/nanostructures and hydrophobic surfaces could be directly constructed on cellulosic paperboard.					
35450633	4	50	theme	low	940:942	arg1	permeabilities					944:957	low permeabilities	940:957	low permeabilities	940:957	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	3	51	theme	dry	720:722	arg1	strength					732:739	impressive dry tensile strength	709:739	impressive dry tensile strength	709:739	The as-obtained multilayered papers demonstrated impressive dry tensile strength and remarkable wet tensile strength of 6542.5 N/m and 5875 N/m, which were increased by 56% and 2277%, respectively.					
35450633	1	52	theme	air	251:253	arg1	properties					263:272	improved moisture and air barrier properties	229:272	properties	263:272	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	4	53	with	paper	888:892	arg1	properties					919:928	rational mechanical properties	899:928	rational mechanical properties	899:928	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	1	54	theme	barrier	255:261	arg1	properties					263:272	improved moisture and air barrier properties	229:272	properties	263:272	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	2	55	theme	cellulose	495:503	arg1	base					511:514	the cellulose paper base	491:514	the cellulose paper base	491:514	Owning to the laminated process followed by spraying approach, MFC and n-SiO2 were assembled onto two surfaces of the cellulose paper base, respectively, endowing the mechanical behaviors and superhydrophobic performance of this biodegradable composite papers as packaging material.					
35450633	4	56	theme	mechanical	908:917	arg1	properties					919:928	rational mechanical properties	899:928	rational mechanical properties	899:928	In addition, the multilayered paper with rational mechanical properties possessed low permeabilities of air (3.17 × 10-3 μm·Pa-1·s-1), oxygen (9.687 cm3·m-2·day-1·atm), and water vapor (378.24 g·m-2·day-1), respectively, as well as a superhydrophobic performance with the contact angle of ~151.2°.					
35450633	2	57	theme	laminated	391:399	arg1	process					401:407	the laminated process	387:407	the laminated process followed by spraying approach	387:437	Owning to the laminated process followed by spraying approach, MFC and n-SiO2 were assembled onto two surfaces of the cellulose paper base, respectively, endowing the mechanical behaviors and superhydrophobic performance of this biodegradable composite papers as packaging material.					
35450633	2	58	dep	behaviors	555:563	arg1	the					540:542	the	540:542	the	540:542	Owning to the laminated process followed by spraying approach, MFC and n-SiO2 were assembled onto two surfaces of the cellulose paper base, respectively, endowing the mechanical behaviors and superhydrophobic performance of this biodegradable composite papers as packaging material.					
35450633	5	59	theme	cellulosic	1389:1398	arg1	paperboard					1400:1409	cellulosic paperboard	1389:1409	cellulosic paperboard	1389:1409	Overall, the feasibility of large-scale production of biodegradable packaging materials in the paper-making industry is demonstrated by the fact that the micro/nanostructures and hydrophobic surfaces could be directly constructed on cellulosic paperboard.					
35450633	1	60	attach	derived	285:291	arg2	moisture					238:245	improved moisture and air barrier properties	229:272	moisture	238:245	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	1	60	attach	derived	285:291	arg1	pulps					309:313	cellulosic pulps	298:313	cellulosic pulps	298:313	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	1	60	attach	derived	285:291	arg2	properties					263:272	improved moisture and air barrier properties	229:272	properties	263:272	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	1	61	with	nano-silica	355:365	arg1	properties					263:272	improved moisture and air barrier properties	229:272	properties	263:272	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35450633	1	61	with	nano-silica	355:365	arg1	moisture					238:245	improved moisture and air barrier properties	229:272	moisture	238:245	Herein, a facile strategy was proposed for preparing a high-strength and super-hydrophobic packaging paper with improved moisture and air barrier properties, which was derived from cellulosic pulps, micro-fibrillated cellulose (MFC), and nano-silica (n-SiO2).					
35753416	5	0	theme	fluorescent	733:743	arg1	signals					745:751	the fluorescent signals	729:751	the fluorescent signals proportional to RAF concentrations at 400 nm	729:796	This sensor turned off the fluorescent signals proportional to RAF concentrations at 400 nm.					
35753416	6	1	theme	detection	882:890	arg1	deviation					987:995	the relative standard deviation	965:995	the relative standard deviation calculated as 1.63% in RAF measuring	965:1032	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	6	1	theme	detection	882:890	arg1	11 × 10-7 M					942:952	11 × 10-7 M	942:952	11 × 10-7 M	942:952	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	6	1	theme	detection	882:890	arg1	34 × 10-8					928:936	34 × 10-8	928:936	34 × 10-8	928:936	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	6	1	theme	detection	882:890	arg1	Limits					872:877	5 × 10-7 to 5 × 10-5 M. Limits	848:877	5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification	848:909	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	9	2	theme	satisfactory	1441:1452	arg1	recoveries					1454:1463	satisfactory recoveries	1441:1463	satisfactory recoveries between 97.5% and 109%	1441:1486	The sensor was applied to determine the RAF concentrations in biological and environmental samples with satisfactory recoveries between 97.5% and 109%.					
35753416	1	3	theme	simple	155:160	arg1	assay					188:192	a simple, applicable, and reliable assay	153:192	a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF)	153:228	It is essential to develop a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF) because of its significant usage and side effect due to entering residue in the environment.					
35753416	6	4	theme	relative	969:976	arg1	deviation					987:995	the relative standard deviation	965:995	the relative standard deviation calculated as 1.63% in RAF measuring	965:1032	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	6	4	theme	relative	969:976	arg1	Limits					872:877	5 × 10-7 to 5 × 10-5 M. Limits	848:877	5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification	848:909	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	1	5	from	residue	295:301	arg1	environment					310:320	the environment	306:320	the environment	306:320	It is essential to develop a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF) because of its significant usage and side effect due to entering residue in the environment.					
35753416	9	6	theme	RAF	1377:1379	arg1	concentrations					1381:1394	the RAF concentrations	1373:1394	the RAF concentrations in biological and environmental samples with satisfactory recoveries between 97.5% and 109%	1373:1486	The sensor was applied to determine the RAF concentrations in biological and environmental samples with satisfactory recoveries between 97.5% and 109%.					
35753416	2	7	theme	Fluorescence	323:334	arg1	sensors					336:342	Fluorescence sensors	323:342	Fluorescence sensors	323:342	Fluorescence sensors developed and widely used because of them high selectivity, fast-response, and highly-sensitivity.					
35753416	0	8	theme	chitosan	107:114	arg1	hydrogel					116:123	chitosan hydrogel	107:123	chitosan hydrogel	107:123	Simple turn-off fluorescence sensor for determination of raloxifene using gold nanoparticles stabilized by chitosan hydrogel.					
35753416	9	9	with	samples	1428:1434	arg1	recoveries					1454:1463	satisfactory recoveries	1441:1463	satisfactory recoveries between 97.5% and 109%	1441:1486	The sensor was applied to determine the RAF concentrations in biological and environmental samples with satisfactory recoveries between 97.5% and 109%.					
35753416	8	10	from	parameters	1186:1195	arg1	determination					1204:1216	RAF determination	1200:1216	RAF determination	1200:1216	The effective parameters in RAF determination, include analyte concentration, temperature, contact time, and pH, were obtained as 35 μM, 30 °C, 8 min, and pH = 8.5.					
35753416	7	11	with	design	1118:1123	arg1	RSM					1166:1168	RSM	1166:1168	RSM	1166:1168	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	7	11	with	design	1118:1123	arg1	methodology					1153:1163	response surface methodology	1136:1163	response surface methodology (RSM)	1136:1169	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	1	12	theme	significant	245:255	arg1	usage					257:261	its significant usage	241:261	its significant usage	241:261	It is essential to develop a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF) because of its significant usage and side effect due to entering residue in the environment.					
35753416	7	13	theme	quenching	1063:1071	arg1	efficiency					1073:1082	quenching efficiency	1063:1082	quenching efficiency	1063:1082	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	8	14	theme	analyte	1227:1233	arg1	concentration					1235:1247	analyte concentration	1227:1247	analyte concentration	1227:1247	The effective parameters in RAF determination, include analyte concentration, temperature, contact time, and pH, were obtained as 35 μM, 30 °C, 8 min, and pH = 8.5.					
35753416	1	15	theme	applicable	163:172	arg1	assay					188:192	a simple, applicable, and reliable assay	153:192	a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF)	153:228	It is essential to develop a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF) because of its significant usage and side effect due to entering residue in the environment.					
35753416	7	16	theme	composite	1108:1116	arg1	CCD					1126:1128	CCD	1126:1128	CCD	1126:1128	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	7	16	theme	composite	1108:1116	arg1	design					1118:1123	central composite design	1100:1123	central composite design (CCD) with response surface methodology (RSM)	1100:1169	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	6	17	from	%	1015:1015	arg1	RAF					1020:1022	RAF measuring	1020:1032	RAF measuring	1020:1032	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	3	18	theme	fluorescence	523:534	arg1	sensor					536:541	a fluorescence sensor	521:541	a fluorescence sensor to determine the trace amount of RAF	521:578	The gold nanoparticles using chitosan hydrogel was synthesized and applied as a fluorescence sensor to determine the trace amount of RAF.					
35753416	3	18	theme	fluorescence	523:534	arg1	nanoparticles					452:464	The gold nanoparticles	443:464	The gold nanoparticles using chitosan hydrogel	443:488	The gold nanoparticles using chitosan hydrogel was synthesized and applied as a fluorescence sensor to determine the trace amount of RAF.					
35753416	6	19	dep	range	837:841	arg1	obtained					916:923	obtained	916:923	were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring	911:1032	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	0	20	theme	turn-off	7:14	arg1	sensor					29:34	Simple turn-off fluorescence sensor	0:34	Simple turn-off fluorescence sensor for determination of raloxifene using gold	0:77	Simple turn-off fluorescence sensor for determination of raloxifene using gold nanoparticles stabilized by chitosan hydrogel.					
35753416	5	21	from	400 nm	791:796	arg1	concentrations					773:786	RAF concentrations	769:786	RAF concentrations at 400 nm	769:796	This sensor turned off the fluorescent signals proportional to RAF concentrations at 400 nm.					
35753416	3	22	theme	trace	560:564	arg1	RAF					576:578	RAF	576:578	RAF	576:578	The gold nanoparticles using chitosan hydrogel was synthesized and applied as a fluorescence sensor to determine the trace amount of RAF.					
35753416	3	22	theme	trace	560:564	arg1	amount					566:571	the trace amount	556:571	the trace amount of RAF	556:578	The gold nanoparticles using chitosan hydrogel was synthesized and applied as a fluorescence sensor to determine the trace amount of RAF.					
35753416	0	23	theme	Simple	0:5	arg1	sensor					29:34	Simple turn-off fluorescence sensor	0:34	Simple turn-off fluorescence sensor for determination of raloxifene using gold	0:77	Simple turn-off fluorescence sensor for determination of raloxifene using gold nanoparticles stabilized by chitosan hydrogel.					
35753416	7	24	from	parameters	1049:1058	arg1	efficiency					1073:1082	quenching efficiency	1063:1082	quenching efficiency	1063:1082	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	4	25	theme	synthesized	683:693	arg1	structure					695:703	the synthesized structure	679:703	the synthesized structure	679:703	The characterization methods including DLS, FE-SEM, EDX, XRD, and FT-IR were performed to confirm the synthesized structure.					
35753416	0	26	dep	nanoparticles	79:91	arg1	stabilized					93:102	stabilized	93:102	nanoparticles stabilized by chitosan hydrogel	79:123	Simple turn-off fluorescence sensor for determination of raloxifene using gold nanoparticles stabilized by chitosan hydrogel.					
35753416	1	27	theme	reliable	179:186	arg1	assay					188:192	a simple, applicable, and reliable assay	153:192	a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF)	153:228	It is essential to develop a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF) because of its significant usage and side effect due to entering residue in the environment.					
35753416	8	28	theme	contact	1263:1269	arg1	time					1271:1274	contact time	1263:1274	contact time	1263:1274	The effective parameters in RAF determination, include analyte concentration, temperature, contact time, and pH, were obtained as 35 μM, 30 °C, 8 min, and pH = 8.5.					
35753416	3	29	theme	gold	447:450	arg1	sensor					536:541	a fluorescence sensor	521:541	a fluorescence sensor to determine the trace amount of RAF	521:578	The gold nanoparticles using chitosan hydrogel was synthesized and applied as a fluorescence sensor to determine the trace amount of RAF.					
35753416	3	29	theme	gold	447:450	arg1	nanoparticles					452:464	The gold nanoparticles	443:464	The gold nanoparticles using chitosan hydrogel	443:488	The gold nanoparticles using chitosan hydrogel was synthesized and applied as a fluorescence sensor to determine the trace amount of RAF.					
35753416	0	30	theme	fluorescence	16:27	arg1	sensor					29:34	Simple turn-off fluorescence sensor	0:34	Simple turn-off fluorescence sensor for determination of raloxifene using gold	0:77	Simple turn-off fluorescence sensor for determination of raloxifene using gold nanoparticles stabilized by chitosan hydrogel.					
35753416	1	31	theme	side	267:270	arg1	effect					272:277	side effect	267:277	side effect	267:277	It is essential to develop a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF) because of its significant usage and side effect due to entering residue in the environment.					
35753416	7	32	theme	effective	1039:1047	arg1	parameters					1049:1058	The effective parameters	1035:1058	The effective parameters on quenching efficiency	1035:1082	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	5	33	theme	proportional	753:764	arg1	signals					745:751	the fluorescent signals	729:751	the fluorescent signals proportional to RAF concentrations at 400 nm	729:796	This sensor turned off the fluorescent signals proportional to RAF concentrations at 400 nm.					
35753416	1	34	theme	anticancer	197:206	arg1	RAF					225:227	RAF	225:227	RAF	225:227	It is essential to develop a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF) because of its significant usage and side effect due to entering residue in the environment.					
35753416	1	34	theme	anticancer	197:206	arg1	raloxifene					213:222	anticancer drug raloxifene	197:222	anticancer drug raloxifene (RAF)	197:228	It is essential to develop a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF) because of its significant usage and side effect due to entering residue in the environment.					
35753416	6	35	theme	measuring	1024:1032	arg1	RAF					1020:1022	RAF measuring	1020:1032	RAF measuring	1020:1032	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	2	36	theme	high	386:389	arg1	selectivity					391:401	high selectivity	386:401	high selectivity	386:401	Fluorescence sensors developed and widely used because of them high selectivity, fast-response, and highly-sensitivity.					
35753416	8	37	theme	RAF	1200:1202	arg1	determination					1204:1216	RAF determination	1200:1216	RAF determination	1200:1216	The effective parameters in RAF determination, include analyte concentration, temperature, contact time, and pH, were obtained as 35 μM, 30 °C, 8 min, and pH = 8.5.					
35753416	1	38	theme	drug	208:211	arg1	RAF					225:227	RAF	225:227	RAF	225:227	It is essential to develop a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF) because of its significant usage and side effect due to entering residue in the environment.					
35753416	1	38	theme	drug	208:211	arg1	raloxifene					213:222	anticancer drug raloxifene	197:222	anticancer drug raloxifene (RAF)	197:228	It is essential to develop a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF) because of its significant usage and side effect due to entering residue in the environment.					
35753416	0	39	theme	raloxifene	57:66	arg1	determination					40:52	determination	40:52	determination of raloxifene using gold	40:77	Simple turn-off fluorescence sensor for determination of raloxifene using gold nanoparticles stabilized by chitosan hydrogel.					
35753416	9	40	theme	biological	1399:1408	arg1	samples					1428:1434	biological and environmental samples	1399:1434	biological and environmental samples with satisfactory recoveries between 97.5% and 109%	1399:1486	The sensor was applied to determine the RAF concentrations in biological and environmental samples with satisfactory recoveries between 97.5% and 109%.					
35753416	9	41	theme	environmental	1414:1426	arg1	samples					1428:1434	biological and environmental samples	1399:1434	biological and environmental samples with satisfactory recoveries between 97.5% and 109%	1399:1486	The sensor was applied to determine the RAF concentrations in biological and environmental samples with satisfactory recoveries between 97.5% and 109%.					
35753416	8	42	theme	effective	1176:1184	arg1	pH = 8.5					1327:1334	pH = 8.5	1327:1334	pH = 8.5	1327:1334	The effective parameters in RAF determination, include analyte concentration, temperature, contact time, and pH, were obtained as 35 μM, 30 °C, 8 min, and pH = 8.5.					
35753416	8	42	theme	effective	1176:1184	arg1	30 °C					1309:1313	30 °C	1309:1313	30 °C	1309:1313	The effective parameters in RAF determination, include analyte concentration, temperature, contact time, and pH, were obtained as 35 μM, 30 °C, 8 min, and pH = 8.5.					
35753416	8	42	theme	effective	1176:1184	arg1	35 μM					1302:1306	35 μM	1302:1306	35 μM	1302:1306	The effective parameters in RAF determination, include analyte concentration, temperature, contact time, and pH, were obtained as 35 μM, 30 °C, 8 min, and pH = 8.5.					
35753416	8	42	theme	effective	1176:1184	arg1	8 min					1316:1320	8 min	1316:1320	8 min	1316:1320	The effective parameters in RAF determination, include analyte concentration, temperature, contact time, and pH, were obtained as 35 μM, 30 °C, 8 min, and pH = 8.5.					
35753416	8	42	theme	effective	1176:1184	arg1	parameters					1186:1195	The effective parameters	1172:1195	The effective parameters	1172:1195	The effective parameters in RAF determination, include analyte concentration, temperature, contact time, and pH, were obtained as 35 μM, 30 °C, 8 min, and pH = 8.5.					
35753416	1	43	theme	entering	286:293	arg1	residue					295:301	entering residue	286:301	entering residue in the environment	286:320	It is essential to develop a simple, applicable, and reliable assay to anticancer drug raloxifene (RAF) because of its significant usage and side effect due to entering residue in the environment.					
35753416	8	44	dep	parameters	1186:1195	arg1	include					1219:1225	include	1219:1225	include analyte concentration, temperature, contact time, and pH	1219:1282	The effective parameters in RAF determination, include analyte concentration, temperature, contact time, and pH, were obtained as 35 μM, 30 °C, 8 min, and pH = 8.5.					
35753416	3	45	theme	RAF	576:578	arg1	RAF					576:578	RAF	576:578	RAF	576:578	The gold nanoparticles using chitosan hydrogel was synthesized and applied as a fluorescence sensor to determine the trace amount of RAF.					
35753416	3	45	theme	RAF	576:578	arg1	amount					566:571	the trace amount	556:571	the trace amount of RAF	556:578	The gold nanoparticles using chitosan hydrogel was synthesized and applied as a fluorescence sensor to determine the trace amount of RAF.					
35753416	9	46	from	concentrations	1381:1394	arg1	samples					1428:1434	biological and environmental samples	1399:1434	biological and environmental samples with satisfactory recoveries between 97.5% and 109%	1399:1486	The sensor was applied to determine the RAF concentrations in biological and environmental samples with satisfactory recoveries between 97.5% and 109%.					
35753416	6	47	located	detected	814:821	arg2	RAF					803:805	The RAF	799:805	The RAF	799:805	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	6	47	located	detected	814:821	arg1	range					837:841	the linear range	826:841	the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring	826:1032	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	7	48	theme	central	1100:1106	arg1	CCD					1126:1128	CCD	1126:1128	CCD	1126:1128	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	7	48	theme	central	1100:1106	arg1	design					1118:1123	central composite design	1100:1123	central composite design (CCD) with response surface methodology (RSM)	1100:1169	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	6	49	theme	quantification	896:909	arg1	deviation					987:995	the relative standard deviation	965:995	the relative standard deviation calculated as 1.63% in RAF measuring	965:1032	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	6	49	theme	quantification	896:909	arg1	11 × 10-7 M					942:952	11 × 10-7 M	942:952	11 × 10-7 M	942:952	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	6	49	theme	quantification	896:909	arg1	34 × 10-8					928:936	34 × 10-8	928:936	34 × 10-8	928:936	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	6	49	theme	quantification	896:909	arg1	Limits					872:877	5 × 10-7 to 5 × 10-5 M. Limits	848:877	5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification	848:909	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	3	50	theme	chitosan	472:479	arg1	hydrogel					481:488	chitosan hydrogel	472:488	chitosan hydrogel	472:488	The gold nanoparticles using chitosan hydrogel was synthesized and applied as a fluorescence sensor to determine the trace amount of RAF.					
35753416	4	51	theme	characterization	585:600	arg1	methods					602:608	The characterization methods	581:608	The characterization methods including DLS, FE-SEM, EDX, XRD, and FT-IR	581:651	The characterization methods including DLS, FE-SEM, EDX, XRD, and FT-IR were performed to confirm the synthesized structure.					
35753416	6	52	theme	linear	830:835	arg1	range					837:841	the linear range	826:841	the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring	826:1032	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	2	53	used	used	365:368	arg2	sensors					336:342	Fluorescence sensors	323:342	Fluorescence sensors	323:342	Fluorescence sensors developed and widely used because of them high selectivity, fast-response, and highly-sensitivity.					
35753416	6	54	dep	5 × 10-5 M.	860:870	arg1	to					857:858	to	857:858	to	857:858	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	5	55	theme	RAF	769:771	arg1	concentrations					773:786	RAF concentrations	769:786	RAF concentrations at 400 nm	769:796	This sensor turned off the fluorescent signals proportional to RAF concentrations at 400 nm.					
35753416	7	56	theme	response	1136:1143	arg1	RSM					1166:1168	RSM	1166:1168	RSM	1166:1168	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	7	56	theme	response	1136:1143	arg1	methodology					1153:1163	response surface methodology	1136:1163	response surface methodology (RSM)	1136:1169	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	7	57	theme	surface	1145:1151	arg1	RSM					1166:1168	RSM	1166:1168	RSM	1166:1168	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	7	57	theme	surface	1145:1151	arg1	methodology					1153:1163	response surface methodology	1136:1163	response surface methodology (RSM)	1136:1169	The effective parameters on quenching efficiency were studied by central composite design (CCD) with response surface methodology (RSM).					
35753416	6	58	theme	standard	978:985	arg1	deviation					987:995	the relative standard deviation	965:995	the relative standard deviation calculated as 1.63% in RAF measuring	965:1032	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
35753416	6	58	theme	standard	978:985	arg1	Limits					872:877	5 × 10-7 to 5 × 10-5 M. Limits	848:877	5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification	848:909	The RAF can be detected in the linear range from 5 × 10-7 to 5 × 10-5 M. Limits of detection and quantification were obtained as 34 × 10-8 and 11 × 10-7 M as well as the relative standard deviation calculated as 1.63% in RAF measuring.					
37381888	4	0	theme	factor	981:986	arg1	hydrogel					904:911	the outer sodium alginate hydrogel	878:911	the outer sodium alginate hydrogel (SA)	878:916	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	0	theme	factor	981:986	arg1	platform					1003:1010	a recombinant human epidermal growth factor (rhEGF)-loaded platform	944:1010	a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel	944:1066	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	1	theme	itg-PEGDA	743:751	arg1	SA					753:754	itg-PEGDA@SA	743:754	itg-PEGDA@SA	743:754	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	1	theme	itg-PEGDA	743:751	arg1	hydrogel					733:740	an integrated polyethylene glycol/alginate-based hydrogel	684:740	an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA)	684:755	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	2	theme	outer	882:886	arg1	SA					914:915	SA	914:915	SA	914:915	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	2	theme	outer	882:886	arg1	hydrogel					904:911	the outer sodium alginate hydrogel	878:911	the outer sodium alginate hydrogel (SA)	878:916	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	2	theme	outer	882:886	arg1	platform					1003:1010	a recombinant human epidermal growth factor (rhEGF)-loaded platform	944:1010	a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel	944:1066	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	5	3	theme	SA	1095:1096	arg1	hydrogel					1098:1105	the itg-PEGDA@SA hydrogel	1081:1105	the itg-PEGDA@SA hydrogel	1081:1105	Altogether, the itg-PEGDA@SA hydrogel showed significant ROS consumption and biocompatibility in vitro, and when applied for wound healing, the formation of uniform and ordered collagen fibers (stained using aniline blue) can be achieved.					
37381888	2	4	theme	consumption	346:356	arg1	capabilities					358:369	sensitive ROS consumption capabilities	332:369	sensitive ROS consumption capabilities	332:369	Therefore, many hydrogels with sensitive ROS consumption capabilities and antibacterial properties have been widely developed and applied.					
37381888	6	5	theme	wound	1415:1419	arg1	dressings					1421:1429	wound dressings	1415:1429	wound dressings	1415:1429	This hydrogel showed favorable attributes in ROS scavenging, and it can be a promising material for use in wound dressings and biomaterial fields.					
37381888	3	6	contain	have	623:626	arg1	materials					557:565	these materials	551:565	these materials	551:565	These hydrogels usually achieve their ROS consumption capacity by introducing reactive active groups: however, these materials normally require complicated preparation procedures and have high potential toxicity.					
37381888	3	6	contain	have	623:626	arg2	toxicity					643:650	high potential toxicity	628:650	high potential toxicity	628:650	These hydrogels usually achieve their ROS consumption capacity by introducing reactive active groups: however, these materials normally require complicated preparation procedures and have high potential toxicity.					
37381888	0	7	theme	species	97:103	arg1	consumption					105:115	significant reactive oxygen species consumption	69:115	significant reactive oxygen species consumption for promoting wound healing	69:143	A biocompatible polyethylene glycol/alginate composite hydrogel with significant reactive oxygen species consumption for promoting wound healing.					
37381888	5	8	theme	significant	1114:1124	arg1	consumption					1130:1140	significant ROS consumption	1114:1140	significant ROS consumption	1114:1140	Altogether, the itg-PEGDA@SA hydrogel showed significant ROS consumption and biocompatibility in vitro, and when applied for wound healing, the formation of uniform and ordered collagen fibers (stained using aniline blue) can be achieved.					
37381888	2	9	theme	ROS	342:344	arg1	capabilities					358:369	sensitive ROS consumption capabilities	332:369	sensitive ROS consumption capabilities	332:369	Therefore, many hydrogels with sensitive ROS consumption capabilities and antibacterial properties have been widely developed and applied.					
37381888	4	10	theme	composite	1049:1057	arg1	hydrogel					1059:1066	this composite hydrogel	1044:1066	this composite hydrogel	1044:1066	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	1	11	theme	tissue	255:260	arg1	damage					262:267	tissue damage	255:267	tissue damage	255:267	In areas of wound inflammation, excessive reactive oxygen species (ROS) may worsen the infection and lead to tissue damage, resulting in a vicious circle.					
37381888	5	12	theme	ROS	1126:1128	arg1	consumption					1130:1140	significant ROS consumption	1114:1140	significant ROS consumption	1114:1140	Altogether, the itg-PEGDA@SA hydrogel showed significant ROS consumption and biocompatibility in vitro, and when applied for wound healing, the formation of uniform and ordered collagen fibers (stained using aniline blue) can be achieved.					
37381888	4	13	theme	alginate	895:902	arg1	SA					914:915	SA	914:915	SA	914:915	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	13	theme	alginate	895:902	arg1	hydrogel					904:911	the outer sodium alginate hydrogel	878:911	the outer sodium alginate hydrogel (SA)	878:916	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	13	theme	alginate	895:902	arg1	platform					1003:1010	a recombinant human epidermal growth factor (rhEGF)-loaded platform	944:1010	a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel	944:1066	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	3	14	theme	complicated	584:594	arg1	procedures					608:617	complicated preparation procedures	584:617	complicated preparation procedures	584:617	These hydrogels usually achieve their ROS consumption capacity by introducing reactive active groups: however, these materials normally require complicated preparation procedures and have high potential toxicity.					
37381888	4	15	theme	-loaded	995:1001	arg1	hydrogel					904:911	the outer sodium alginate hydrogel	878:911	the outer sodium alginate hydrogel (SA)	878:916	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	15	theme	-loaded	995:1001	arg1	platform					1003:1010	a recombinant human epidermal growth factor (rhEGF)-loaded platform	944:1010	a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel	944:1066	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	6	16	theme	favorable	1329:1337	arg1	attributes					1339:1348	favorable attributes	1329:1348	favorable attributes in ROS scavenging	1329:1366	This hydrogel showed favorable attributes in ROS scavenging, and it can be a promising material for use in wound dressings and biomaterial fields.					
37381888	4	17	theme	sodium	888:893	arg1	SA					914:915	SA	914:915	SA	914:915	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	17	theme	sodium	888:893	arg1	hydrogel					904:911	the outer sodium alginate hydrogel	878:911	the outer sodium alginate hydrogel (SA)	878:916	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	17	theme	sodium	888:893	arg1	platform					1003:1010	a recombinant human epidermal growth factor (rhEGF)-loaded platform	944:1010	a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel	944:1066	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	5	18	dep	fibers	1255:1260	arg1	stained					1263:1269	stained	1263:1269	stained using aniline blue	1263:1288	Altogether, the itg-PEGDA@SA hydrogel showed significant ROS consumption and biocompatibility in vitro, and when applied for wound healing, the formation of uniform and ordered collagen fibers (stained using aniline blue) can be achieved.					
37381888	3	19	theme	active	527:532	arg1	groups					534:539	reactive active groups	518:539	reactive active groups	518:539	These hydrogels usually achieve their ROS consumption capacity by introducing reactive active groups: however, these materials normally require complicated preparation procedures and have high potential toxicity.					
37381888	4	20	theme	@	752:752	arg1	SA					753:754	itg-PEGDA@SA	743:754	itg-PEGDA@SA	743:754	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	20	theme	@	752:752	arg1	hydrogel					733:740	an integrated polyethylene glycol/alginate-based hydrogel	684:740	an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA)	684:755	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	5	21	theme	itg-PEGDA	1085:1093	arg1	hydrogel					1098:1105	the itg-PEGDA@SA hydrogel	1081:1105	the itg-PEGDA@SA hydrogel	1081:1105	Altogether, the itg-PEGDA@SA hydrogel showed significant ROS consumption and biocompatibility in vitro, and when applied for wound healing, the formation of uniform and ordered collagen fibers (stained using aniline blue) can be achieved.					
37381888	4	22	theme	ROS	860:862	arg1	scavenger					864:872	a ROS scavenger	858:872	a ROS scavenger	858:872	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	22	theme	ROS	860:862	arg1	hydrogel					829:836	the inner PEGDA hydrogel	813:836	the inner PEGDA hydrogel (hdg-PEGDA)	813:848	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	5	23	theme	wound	1194:1198	arg1	healing					1200:1206	wound healing	1194:1206	wound healing	1194:1206	Altogether, the itg-PEGDA@SA hydrogel showed significant ROS consumption and biocompatibility in vitro, and when applied for wound healing, the formation of uniform and ordered collagen fibers (stained using aniline blue) can be achieved.					
37381888	1	24	theme	reactive	188:195	arg1	species					204:210	excessive reactive oxygen species	178:210	excessive reactive oxygen species (ROS)	178:216	In areas of wound inflammation, excessive reactive oxygen species (ROS) may worsen the infection and lead to tissue damage, resulting in a vicious circle.					
37381888	1	24	theme	reactive	188:195	arg1	ROS					213:215	ROS	213:215	ROS	213:215	In areas of wound inflammation, excessive reactive oxygen species (ROS) may worsen the infection and lead to tissue damage, resulting in a vicious circle.					
37381888	0	25	theme	wound	131:135	arg1	healing					137:143	wound healing	131:143	wound healing	131:143	A biocompatible polyethylene glycol/alginate composite hydrogel with significant reactive oxygen species consumption for promoting wound healing.					
37381888	5	26	theme	@	1094:1094	arg1	hydrogel					1098:1105	the itg-PEGDA@SA hydrogel	1081:1105	the itg-PEGDA@SA hydrogel	1081:1105	Altogether, the itg-PEGDA@SA hydrogel showed significant ROS consumption and biocompatibility in vitro, and when applied for wound healing, the formation of uniform and ordered collagen fibers (stained using aniline blue) can be achieved.					
37381888	1	27	theme	oxygen	197:202	arg1	species					204:210	excessive reactive oxygen species	178:210	excessive reactive oxygen species (ROS)	178:216	In areas of wound inflammation, excessive reactive oxygen species (ROS) may worsen the infection and lead to tissue damage, resulting in a vicious circle.					
37381888	1	27	theme	oxygen	197:202	arg1	ROS					213:215	ROS	213:215	ROS	213:215	In areas of wound inflammation, excessive reactive oxygen species (ROS) may worsen the infection and lead to tissue damage, resulting in a vicious circle.					
37381888	0	28	theme	biocompatible	2:14	arg1	polyethylene					16:27	A biocompatible polyethylene	0:27	A biocompatible polyethylene	0:27	A biocompatible polyethylene glycol/alginate composite hydrogel with significant reactive oxygen species consumption for promoting wound healing.					
37381888	4	29	theme	growth	974:979	arg1	rhEGF					989:993	rhEGF	989:993	rhEGF	989:993	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	29	theme	growth	974:979	arg1	factor					981:986	human epidermal growth factor	958:986	a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel	944:1066	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	30	theme	epidermal	964:972	arg1	rhEGF					989:993	rhEGF	989:993	rhEGF	989:993	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	30	theme	epidermal	964:972	arg1	factor					981:986	human epidermal growth factor	958:986	a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel	944:1066	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	3	31	theme	preparation	596:606	arg1	procedures					608:617	complicated preparation procedures	584:617	complicated preparation procedures	584:617	These hydrogels usually achieve their ROS consumption capacity by introducing reactive active groups: however, these materials normally require complicated preparation procedures and have high potential toxicity.					
37381888	4	32	theme	polyethylene	698:709	arg1	SA					753:754	itg-PEGDA@SA	743:754	itg-PEGDA@SA	743:754	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	32	theme	polyethylene	698:709	arg1	hydrogel					733:740	an integrated polyethylene glycol/alginate-based hydrogel	684:740	an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA)	684:755	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	2	33	theme	antibacterial	375:387	arg1	properties					389:398	antibacterial properties	375:398	antibacterial properties	375:398	Therefore, many hydrogels with sensitive ROS consumption capabilities and antibacterial properties have been widely developed and applied.					
37381888	6	34	theme	promising	1385:1393	arg1	material					1395:1402	a promising material	1383:1402	a promising material for use in wound dressings and biomaterial fields	1383:1452	This hydrogel showed favorable attributes in ROS scavenging, and it can be a promising material for use in wound dressings and biomaterial fields.					
37381888	6	34	theme	promising	1385:1393	arg1	it					1373:1374	it	1373:1374	it	1373:1374	This hydrogel showed favorable attributes in ROS scavenging, and it can be a promising material for use in wound dressings and biomaterial fields.					
37381888	4	35	theme	two-step	789:796	arg1	process					798:804	a simple two-step process	780:804	a simple two-step process	780:804	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	3	36	theme	ROS	478:480	arg1	capacity					494:501	their ROS consumption capacity	472:501	their ROS consumption capacity	472:501	These hydrogels usually achieve their ROS consumption capacity by introducing reactive active groups: however, these materials normally require complicated preparation procedures and have high potential toxicity.					
37381888	2	37	with	hydrogels	317:325	arg1	capabilities					358:369	sensitive ROS consumption capabilities	332:369	sensitive ROS consumption capabilities	332:369	Therefore, many hydrogels with sensitive ROS consumption capabilities and antibacterial properties have been widely developed and applied.					
37381888	2	37	with	hydrogels	317:325	arg1	properties					389:398	antibacterial properties	375:398	antibacterial properties	375:398	Therefore, many hydrogels with sensitive ROS consumption capabilities and antibacterial properties have been widely developed and applied.					
37381888	1	38	theme	excessive	178:186	arg1	species					204:210	excessive reactive oxygen species	178:210	excessive reactive oxygen species (ROS)	178:216	In areas of wound inflammation, excessive reactive oxygen species (ROS) may worsen the infection and lead to tissue damage, resulting in a vicious circle.					
37381888	1	38	theme	excessive	178:186	arg1	ROS					213:215	ROS	213:215	ROS	213:215	In areas of wound inflammation, excessive reactive oxygen species (ROS) may worsen the infection and lead to tissue damage, resulting in a vicious circle.					
37381888	3	39	theme	consumption	482:492	arg1	capacity					494:501	their ROS consumption capacity	472:501	their ROS consumption capacity	472:501	These hydrogels usually achieve their ROS consumption capacity by introducing reactive active groups: however, these materials normally require complicated preparation procedures and have high potential toxicity.					
37381888	1	40	theme	vicious	285:291	arg1	circle					293:298	a vicious circle	283:298	a vicious circle	283:298	In areas of wound inflammation, excessive reactive oxygen species (ROS) may worsen the infection and lead to tissue damage, resulting in a vicious circle.					
37381888	5	41	theme	ordered	1238:1244	arg1	fibers					1255:1260	uniform and ordered collagen fibers	1226:1260	uniform and ordered collagen fibers (stained using aniline blue)	1226:1289	Altogether, the itg-PEGDA@SA hydrogel showed significant ROS consumption and biocompatibility in vitro, and when applied for wound healing, the formation of uniform and ordered collagen fibers (stained using aniline blue) can be achieved.					
37381888	3	42	theme	high	628:631	arg1	toxicity					643:650	high potential toxicity	628:650	high potential toxicity	628:650	These hydrogels usually achieve their ROS consumption capacity by introducing reactive active groups: however, these materials normally require complicated preparation procedures and have high potential toxicity.					
37381888	4	43	theme	glycol/alginate-based	711:731	arg1	SA					753:754	itg-PEGDA@SA	743:754	itg-PEGDA@SA	743:754	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	43	theme	glycol/alginate-based	711:731	arg1	hydrogel					733:740	an integrated polyethylene glycol/alginate-based hydrogel	684:740	an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA)	684:755	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	0	44	theme	composite	45:53	arg1	hydrogel					55:62	composite hydrogel	45:62	composite hydrogel with significant reactive oxygen species consumption for promoting wound healing	45:143	A biocompatible polyethylene glycol/alginate composite hydrogel with significant reactive oxygen species consumption for promoting wound healing.					
37381888	3	45	theme	potential	633:641	arg1	toxicity					643:650	high potential toxicity	628:650	high potential toxicity	628:650	These hydrogels usually achieve their ROS consumption capacity by introducing reactive active groups: however, these materials normally require complicated preparation procedures and have high potential toxicity.					
37381888	0	46	theme	significant	69:79	arg1	consumption					105:115	significant reactive oxygen species consumption	69:115	significant reactive oxygen species consumption for promoting wound healing	69:143	A biocompatible polyethylene glycol/alginate composite hydrogel with significant reactive oxygen species consumption for promoting wound healing.					
37381888	4	47	theme	human	958:962	arg1	rhEGF					989:993	rhEGF	989:993	rhEGF	989:993	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	47	theme	human	958:962	arg1	factor					981:986	human epidermal growth factor	958:986	a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel	944:1066	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	6	48	from	attributes	1339:1348	arg1	scavenging					1357:1366	ROS scavenging	1353:1366	ROS scavenging	1353:1366	This hydrogel showed favorable attributes in ROS scavenging, and it can be a promising material for use in wound dressings and biomaterial fields.					
37381888	2	49	theme	sensitive	332:340	arg1	capabilities					358:369	sensitive ROS consumption capabilities	332:369	sensitive ROS consumption capabilities	332:369	Therefore, many hydrogels with sensitive ROS consumption capabilities and antibacterial properties have been widely developed and applied.					
37381888	5	50	theme	fibers	1255:1260	arg1	formation					1213:1221	the formation	1209:1221	the formation of uniform and ordered collagen fibers (stained using aniline blue)	1209:1289	Altogether, the itg-PEGDA@SA hydrogel showed significant ROS consumption and biocompatibility in vitro, and when applied for wound healing, the formation of uniform and ordered collagen fibers (stained using aniline blue) can be achieved.					
37381888	3	51	theme	reactive	518:525	arg1	groups					534:539	reactive active groups	518:539	reactive active groups	518:539	These hydrogels usually achieve their ROS consumption capacity by introducing reactive active groups: however, these materials normally require complicated preparation procedures and have high potential toxicity.					
37381888	4	52	theme	recombinant	946:956	arg1	hydrogel					904:911	the outer sodium alginate hydrogel	878:911	the outer sodium alginate hydrogel (SA)	878:916	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	52	theme	recombinant	946:956	arg1	platform					1003:1010	a recombinant human epidermal growth factor (rhEGF)-loaded platform	944:1010	a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel	944:1066	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	6	53	theme	ROS	1353:1355	arg1	scavenging					1357:1366	ROS scavenging	1353:1366	ROS scavenging	1353:1366	This hydrogel showed favorable attributes in ROS scavenging, and it can be a promising material for use in wound dressings and biomaterial fields.					
37381888	4	54	theme	PEGDA	823:827	arg1	hydrogel					829:836	the inner PEGDA hydrogel	813:836	the inner PEGDA hydrogel (hdg-PEGDA)	813:848	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	54	theme	PEGDA	823:827	arg1	scavenger					864:872	a ROS scavenger	858:872	a ROS scavenger	858:872	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	54	theme	PEGDA	823:827	arg1	hdg-PEGDA					839:847	hdg-PEGDA	839:847	hdg-PEGDA	839:847	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	55	theme	hydrogel	1059:1066	arg1	functionality					1027:1039	the functionality	1023:1039	the functionality of this composite hydrogel	1023:1066	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	6	56	from	use	1408:1410	arg1	dressings					1421:1429	wound dressings	1415:1429	wound dressings	1415:1429	This hydrogel showed favorable attributes in ROS scavenging, and it can be a promising material for use in wound dressings and biomaterial fields.					
37381888	6	56	from	use	1408:1410	arg1	fields					1447:1452	biomaterial fields	1435:1452	biomaterial fields	1435:1452	This hydrogel showed favorable attributes in ROS scavenging, and it can be a promising material for use in wound dressings and biomaterial fields.					
37381888	4	57	theme	inner	817:821	arg1	hydrogel					829:836	the inner PEGDA hydrogel	813:836	the inner PEGDA hydrogel (hdg-PEGDA)	813:848	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	57	theme	inner	817:821	arg1	scavenger					864:872	a ROS scavenger	858:872	a ROS scavenger	858:872	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	57	theme	inner	817:821	arg1	hdg-PEGDA					839:847	hdg-PEGDA	839:847	hdg-PEGDA	839:847	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	58	theme	simple	782:787	arg1	process					798:804	a simple two-step process	780:804	a simple two-step process	780:804	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	2	59	theme	many	312:315	arg1	hydrogels					317:325	many hydrogels	312:325	many hydrogels with sensitive ROS consumption capabilities and antibacterial properties	312:398	Therefore, many hydrogels with sensitive ROS consumption capabilities and antibacterial properties have been widely developed and applied.					
37381888	6	60	theme	biomaterial	1435:1445	arg1	fields					1447:1452	biomaterial fields	1435:1452	biomaterial fields	1435:1452	This hydrogel showed favorable attributes in ROS scavenging, and it can be a promising material for use in wound dressings and biomaterial fields.					
37381888	0	61	theme	oxygen	90:95	arg1	species					97:103	reactive oxygen species	81:103	significant reactive oxygen species consumption for promoting wound healing	69:143	A biocompatible polyethylene glycol/alginate composite hydrogel with significant reactive oxygen species consumption for promoting wound healing.					
37381888	5	62	theme	collagen	1246:1253	arg1	fibers					1255:1260	uniform and ordered collagen fibers	1226:1260	uniform and ordered collagen fibers (stained using aniline blue)	1226:1289	Altogether, the itg-PEGDA@SA hydrogel showed significant ROS consumption and biocompatibility in vitro, and when applied for wound healing, the formation of uniform and ordered collagen fibers (stained using aniline blue) can be achieved.					
37381888	1	63	theme	wound	158:162	arg1	inflammation					164:175	wound inflammation	158:175	wound inflammation	158:175	In areas of wound inflammation, excessive reactive oxygen species (ROS) may worsen the infection and lead to tissue damage, resulting in a vicious circle.					
37381888	4	64	theme	integrated	687:696	arg1	SA					753:754	itg-PEGDA@SA	743:754	itg-PEGDA@SA	743:754	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	4	64	theme	integrated	687:696	arg1	hydrogel					733:740	an integrated polyethylene glycol/alginate-based hydrogel	684:740	an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA)	684:755	Inspired by these limitations, an integrated polyethylene glycol/alginate-based hydrogel (itg-PEGDA@SA) has been developed via a simple two-step process, where the inner PEGDA hydrogel (hdg-PEGDA) acts as a ROS scavenger and the outer sodium alginate hydrogel (SA) can be degraded to act as a recombinant human epidermal growth factor (rhEGF)-loaded platform to enhance the functionality of this composite hydrogel.					
37381888	0	65	theme	reactive	81:88	arg1	species					97:103	reactive oxygen species	81:103	significant reactive oxygen species consumption for promoting wound healing	69:143	A biocompatible polyethylene glycol/alginate composite hydrogel with significant reactive oxygen species consumption for promoting wound healing.					
37381888	0	66	with	hydrogel	55:62	arg1	consumption					105:115	significant reactive oxygen species consumption	69:115	significant reactive oxygen species consumption for promoting wound healing	69:143	A biocompatible polyethylene glycol/alginate composite hydrogel with significant reactive oxygen species consumption for promoting wound healing.					
37381888	1	67	theme	inflammation	164:175	arg1	areas					149:153	areas	149:153	areas of wound inflammation	149:175	In areas of wound inflammation, excessive reactive oxygen species (ROS) may worsen the infection and lead to tissue damage, resulting in a vicious circle.					
37381888	5	68	theme	uniform	1226:1232	arg1	fibers					1255:1260	uniform and ordered collagen fibers	1226:1260	uniform and ordered collagen fibers (stained using aniline blue)	1226:1289	Altogether, the itg-PEGDA@SA hydrogel showed significant ROS consumption and biocompatibility in vitro, and when applied for wound healing, the formation of uniform and ordered collagen fibers (stained using aniline blue) can be achieved.					
37187419	1	0	theme	gum	347:349	arg1	biopolymers					358:368	chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers	310:368	chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water	310:377	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	8	1	theme	copper	1445:1450	arg1	adsorption					1452:1461	copper adsorption	1445:1461	copper adsorption	1445:1461	Thermodynamic analysis indicated that copper adsorption occurred spontaneously (ΔG°: -2.85 J/mol, 298 K) and exothermically (ΔH°: -27.58 J/mol).					
37187419	0	2	from	solution	170:177	arg1	adsorption					146:155	Cu2+ adsorption	141:155	Cu2+ adsorption from aqueous solution	141:177	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.					
37187419	0	3	theme	guar	89:92	arg1	gum					94:96	carboxymethyl guar gum	75:96	carboxymethyl guar gum	75:96	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.					
37187419	5	4	theme	Cu	1097:1098	arg1	adsorption					1083:1092	the maximum adsorption	1071:1092	the maximum adsorption of Cu(II)	1071:1102	Using the Langmuir isotherm model for an initial metal concentration of 50 mg/L at pH 6.0 and 25 °C, the maximum adsorption of Cu(II) was calculated to be 155.51 mg/g.					
37187419	5	4	theme	Cu	1097:1098	arg1	155.51 mg/g					1125:1135	155.51 mg/g	1125:1135	155.51 mg/g	1125:1135	Using the Langmuir isotherm model for an initial metal concentration of 50 mg/L at pH 6.0 and 25 °C, the maximum adsorption of Cu(II) was calculated to be 155.51 mg/g.					
37187419	6	5	from	adsorption	1223:1232	arg1	CS/CMGG					1241:1247	the CS/CMGG	1237:1247	the CS/CMGG	1237:1247	A combination of adsorption-complexation and ion exchange must be involved in Cu(II) adsorption on the CS/CMGG.					
37187419	3	6	theme	aqueous	724:730	arg1	solution					732:739	aqueous solution	724:739	aqueous solution	724:739	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	9	7	theme	heavy	1590:1594	arg1	ions					1602:1605	heavy metal ions	1590:1605	heavy metal ions	1590:1605	A reusable bio-adsorbent for removing heavy metal ions was developed that is eco-friendly, sustainable, and efficient.					
37187419	5	8	theme	Langmuir	980:987	arg1	model					998:1002	the Langmuir isotherm model	976:1002	the Langmuir isotherm model for an initial metal concentration of 50 mg/L	976:1048	Using the Langmuir isotherm model for an initial metal concentration of 50 mg/L at pH 6.0 and 25 °C, the maximum adsorption of Cu(II) was calculated to be 155.51 mg/g.					
37187419	8	9	theme	Thermodynamic	1407:1419	arg1	analysis					1421:1428	Thermodynamic analysis	1407:1428	Thermodynamic analysis	1407:1428	Thermodynamic analysis indicated that copper adsorption occurred spontaneously (ΔG°: -2.85 J/mol, 298 K) and exothermically (ΔH°: -27.58 J/mol).					
37187419	6	10	theme	ion	1183:1185	arg1	exchange					1187:1194	ion exchange	1183:1194	ion exchange	1183:1194	A combination of adsorption-complexation and ion exchange must be involved in Cu(II) adsorption on the CS/CMGG.					
37187419	5	11	theme	isotherm	989:996	arg1	model					998:1002	the Langmuir isotherm model	976:1002	the Langmuir isotherm model for an initial metal concentration of 50 mg/L	976:1048	Using the Langmuir isotherm model for an initial metal concentration of 50 mg/L at pH 6.0 and 25 °C, the maximum adsorption of Cu(II) was calculated to be 155.51 mg/g.					
37187419	8	12	dep	ΔH°	1532:1534	arg1	-27.58 J/mol					1537:1548	-27.58 J/mol	1537:1548	ΔH°: -27.58 J/mol	1532:1548	Thermodynamic analysis indicated that copper adsorption occurred spontaneously (ΔG°: -2.85 J/mol, 298 K) and exothermically (ΔH°: -27.58 J/mol).					
37187419	0	13	theme	bio-based	107:115	arg1	composite					117:125	A novel bio-based composite	99:125	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.	0:178	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.					
37187419	7	14	theme	Cu	1380:1381	arg1	percentage					1395:1404	Cu(II) removal percentage	1380:1404	Cu(II) removal percentage	1380:1404	Five cycles of the loaded CS/CMGG hydrogel regeneration and reuse were successfully achieved without an appreciable difference in Cu(II) removal percentage.					
37187419	7	15	theme	hydrogel	1284:1291	arg1	regeneration					1293:1304	the loaded CS/CMGG hydrogel regeneration	1265:1304	the loaded CS/CMGG hydrogel regeneration	1265:1304	Five cycles of the loaded CS/CMGG hydrogel regeneration and reuse were successfully achieved without an appreciable difference in Cu(II) removal percentage.					
37187419	7	16	theme	regeneration	1293:1304	arg1	cycles					1255:1260	Five cycles	1250:1260	Five cycles of the loaded CS/CMGG hydrogel regeneration and reuse	1250:1314	Five cycles of the loaded CS/CMGG hydrogel regeneration and reuse were successfully achieved without an appreciable difference in Cu(II) removal percentage.					
37187419	7	17	theme	reuse	1310:1314	arg1	cycles					1255:1260	Five cycles	1250:1260	Five cycles of the loaded CS/CMGG hydrogel regeneration and reuse	1250:1314	Five cycles of the loaded CS/CMGG hydrogel regeneration and reuse were successfully achieved without an appreciable difference in Cu(II) removal percentage.					
37187419	0	18	theme	novel	101:105	arg1	composite					117:125	A novel bio-based composite	99:125	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.	0:178	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.					
37187419	6	19	theme	adsorption-complexation	1155:1177	arg1	combination					1140:1150	A combination	1138:1150	A combination of adsorption-complexation and ion exchange	1138:1194	A combination of adsorption-complexation and ion exchange must be involved in Cu(II) adsorption on the CS/CMGG.					
37187419	0	20	theme	all-biopolymer	3:16	arg1	film					43:46	An all-biopolymer self-assembling hydrogel film	0:46	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.	0:178	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.					
37187419	4	21	theme	isotherm	870:877	arg1	models					879:884	The pseudo-second-order kinetic and Langmuir isotherm models	825:884	models	879:884	The pseudo-second-order kinetic and Langmuir isotherm models are highly correlated with the kinetic and equilibrium isotherm data, respectively.					
37187419	8	22	dep	and	1512:1514	arg1	ΔH°					1532:1534	ΔH°	1532:1534	ΔH°: -27.58 J/mol	1532:1548	Thermodynamic analysis indicated that copper adsorption occurred spontaneously (ΔG°: -2.85 J/mol, 298 K) and exothermically (ΔH°: -27.58 J/mol).					
37187419	8	22	dep	and	1512:1514	arg1	298 K					1505:1509	298 K	1505:1509	298 K	1505:1509	Thermodynamic analysis indicated that copper adsorption occurred spontaneously (ΔG°: -2.85 J/mol, 298 K) and exothermically (ΔH°: -27.58 J/mol).					
37187419	8	22	dep	and	1512:1514	arg1	ΔG°					1487:1489	ΔG°	1487:1489	ΔG°	1487:1489	Thermodynamic analysis indicated that copper adsorption occurred spontaneously (ΔG°: -2.85 J/mol, 298 K) and exothermically (ΔH°: -27.58 J/mol).					
37187419	1	23	theme	novel	182:186	arg1	film					258:261	all biopolymer self-assembled hydrogel film	219:261	all biopolymer self-assembled hydrogel film	219:261	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	1	23	theme	novel	182:186	arg1	composite					198:206	A novel bio-based composite adsorbent	180:216	A novel bio-based composite adsorbent	180:216	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	8	24	dep	ΔG°	1487:1489	arg1	-2.85 J/mol					1492:1502	-2.85 J/mol	1492:1502	-2.85 J/mol	1492:1502	Thermodynamic analysis indicated that copper adsorption occurred spontaneously (ΔG°: -2.85 J/mol, 298 K) and exothermically (ΔH°: -27.58 J/mol).					
37187419	4	25	theme	Langmuir	861:868	arg1	models					879:884	The pseudo-second-order kinetic and Langmuir isotherm models	825:884	models	879:884	The pseudo-second-order kinetic and Langmuir isotherm models are highly correlated with the kinetic and equilibrium isotherm data, respectively.					
37187419	7	26	theme	removal	1387:1393	arg1	percentage					1395:1404	Cu(II) removal percentage	1380:1404	Cu(II) removal percentage	1380:1404	Five cycles of the loaded CS/CMGG hydrogel regeneration and reuse were successfully achieved without an appreciable difference in Cu(II) removal percentage.					
37187419	1	27	theme	bio-based	188:196	arg1	film					258:261	all biopolymer self-assembled hydrogel film	219:261	all biopolymer self-assembled hydrogel film	219:261	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	1	27	theme	bio-based	188:196	arg1	composite					198:206	A novel bio-based composite adsorbent	180:216	A novel bio-based composite adsorbent	180:216	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	0	28	theme	hydrogel	34:41	arg1	film					43:46	An all-biopolymer self-assembling hydrogel film	0:46	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.	0:178	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.					
37187419	0	29	theme	adsorbent	127:135	arg1	composite					117:125	A novel bio-based composite	99:125	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.	0:178	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.					
37187419	3	30	theme	Various	613:619	arg1	parameters					634:643	Various experimental parameters	613:643	Various experimental parameters	613:643	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	9	31	theme	reusable	1554:1561	arg1	bio-adsorbent					1563:1575	A reusable bio-adsorbent	1552:1575	A reusable bio-adsorbent for removing heavy metal ions	1552:1605	A reusable bio-adsorbent for removing heavy metal ions was developed that is eco-friendly, sustainable, and efficient.					
37187419	7	32	from	difference	1366:1375	arg1	percentage					1395:1404	Cu(II) removal percentage	1380:1404	Cu(II) removal percentage	1380:1404	Five cycles of the loaded CS/CMGG hydrogel regeneration and reuse were successfully achieved without an appreciable difference in Cu(II) removal percentage.					
37187419	2	33	theme	Various	430:436	arg1	analysis					438:445	Various analysis	430:445	Various analysis	430:445	Various analysis demonstrated the electrostatic interactions and hydrogen bondings within the network structure are responsible for gelling, crosslinking, and forming a 3D structure.					
37187419	0	34	theme	self-assembling	18:32	arg1	film					43:46	An all-biopolymer self-assembling hydrogel film	0:46	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.	0:178	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.					
37187419	1	35	theme	chitosan	310:317	arg1	biopolymers					358:368	chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers	310:368	chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water	310:377	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	5	36	theme	maximum	1075:1081	arg1	adsorption					1083:1092	the maximum adsorption	1071:1092	the maximum adsorption of Cu(II)	1071:1102	Using the Langmuir isotherm model for an initial metal concentration of 50 mg/L at pH 6.0 and 25 °C, the maximum adsorption of Cu(II) was calculated to be 155.51 mg/g.					
37187419	5	36	theme	maximum	1075:1081	arg1	155.51 mg/g					1125:1135	155.51 mg/g	1125:1135	155.51 mg/g	1125:1135	Using the Langmuir isotherm model for an initial metal concentration of 50 mg/L at pH 6.0 and 25 °C, the maximum adsorption of Cu(II) was calculated to be 155.51 mg/g.					
37187419	1	37	theme	adsorbent	208:216	arg1	film					258:261	all biopolymer self-assembled hydrogel film	219:261	all biopolymer self-assembled hydrogel film	219:261	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	1	37	theme	adsorbent	208:216	arg1	composite					198:206	A novel bio-based composite adsorbent	180:216	A novel bio-based composite adsorbent	180:216	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	7	38	theme	loaded	1269:1274	arg1	regeneration					1293:1304	the loaded CS/CMGG hydrogel regeneration	1265:1304	the loaded CS/CMGG hydrogel regeneration	1265:1304	Five cycles of the loaded CS/CMGG hydrogel regeneration and reuse were successfully achieved without an appreciable difference in Cu(II) removal percentage.					
37187419	3	39	theme	Cu	764:765	arg1	concentration					779:791	Cu(II) initial concentration	764:791	Cu(II) initial concentration	764:791	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	0	40	theme	Cu2+	141:144	arg1	adsorption					146:155	Cu2+ adsorption	141:155	Cu2+ adsorption from aqueous solution	141:177	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.					
37187419	3	41	theme	experimental	621:632	arg1	parameters					634:643	Various experimental parameters	613:643	Various experimental parameters	613:643	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	2	42	theme	hydrogen	495:502	arg1	bondings					504:511	hydrogen bondings	495:511	hydrogen bondings within the network structure	495:540	Various analysis demonstrated the electrostatic interactions and hydrogen bondings within the network structure are responsible for gelling, crosslinking, and forming a 3D structure.					
37187419	1	43	theme	small	395:399	arg1	molecules					401:409	small molecules	395:409	small molecules for cross-linking	395:427	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	3	44	theme	contact	794:800	arg1	time					802:805	contact time	794:805	contact time	794:805	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	3	45	theme	initial	771:777	arg1	concentration					779:791	Cu(II) initial concentration	764:791	Cu(II) initial concentration	764:791	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	3	46	from	solution	732:739	arg1	pH					752:753	pH	752:753	pH	752:753	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	3	46	from	solution	732:739	arg1	dosage					756:761	dosage	756:761	dosage	756:761	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	3	46	from	solution	732:739	arg1	concentration					779:791	Cu(II) initial concentration	764:791	Cu(II) initial concentration	764:791	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	3	46	from	solution	732:739	arg1	temperature					812:822	temperature	812:822	temperature	812:822	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	3	46	from	solution	732:739	arg1	time					802:805	contact time	794:805	contact time	794:805	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	3	46	from	solution	732:739	arg1	ions					714:717	Cu2+ ions	709:717	Cu2+ ions	709:717	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	4	47	dep	kinetic	917:923	arg1	data					950:953	isotherm data	941:953	isotherm data	941:953	The pseudo-second-order kinetic and Langmuir isotherm models are highly correlated with the kinetic and equilibrium isotherm data, respectively.					
37187419	4	47	dep	kinetic	917:923	arg1	the					913:915	the	913:915	the	913:915	The pseudo-second-order kinetic and Langmuir isotherm models are highly correlated with the kinetic and equilibrium isotherm data, respectively.					
37187419	7	48	theme	appreciable	1354:1364	arg1	difference					1366:1375	an appreciable difference	1351:1375	an appreciable difference in Cu(II) removal percentage	1351:1404	Five cycles of the loaded CS/CMGG hydrogel regeneration and reuse were successfully achieved without an appreciable difference in Cu(II) removal percentage.					
37187419	2	49	theme	3D	599:600	arg1	structure					602:610	a 3D structure	597:610	a 3D structure	597:610	Various analysis demonstrated the electrostatic interactions and hydrogen bondings within the network structure are responsible for gelling, crosslinking, and forming a 3D structure.					
37187419	3	50	theme	Cu2+	709:712	arg1	pH					752:753	pH	752:753	pH	752:753	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	3	50	theme	Cu2+	709:712	arg1	dosage					756:761	dosage	756:761	dosage	756:761	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	3	50	theme	Cu2+	709:712	arg1	concentration					779:791	Cu(II) initial concentration	764:791	Cu(II) initial concentration	764:791	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	3	50	theme	Cu2+	709:712	arg1	temperature					812:822	temperature	812:822	temperature	812:822	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	3	50	theme	Cu2+	709:712	arg1	time					802:805	contact time	794:805	contact time	794:805	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	3	50	theme	Cu2+	709:712	arg1	ions					714:717	Cu2+ ions	709:717	Cu2+ ions	709:717	Various experimental parameters were optimized to evaluate the CS/CMGG's potential for removing Cu2+ ions from aqueous solution, including pH, dosage, Cu(II) initial concentration, contact time, and temperature.					
37187419	0	51	dep	film	43:46	arg1	composite					117:125	A novel bio-based composite	99:125	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.	0:178	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.					
37187419	6	52	theme	Cu	1216:1217	arg1	adsorption					1223:1232	Cu(II) adsorption	1216:1232	Cu(II) adsorption on the CS/CMGG	1216:1247	A combination of adsorption-complexation and ion exchange must be involved in Cu(II) adsorption on the CS/CMGG.					
37187419	1	53	theme	carboxymethyl	328:340	arg1	CMGG					352:355	CMGG	352:355	CMGG	352:355	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	1	53	theme	carboxymethyl	328:340	arg1	gum					347:349	carboxymethyl guar gum	328:349	carboxymethyl guar gum (CMGG)	328:356	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	5	54	theme	50 mg/L	1042:1048	arg1	concentration					1025:1037	an initial metal concentration	1008:1037	an initial metal concentration of 50 mg/L	1008:1048	Using the Langmuir isotherm model for an initial metal concentration of 50 mg/L at pH 6.0 and 25 °C, the maximum adsorption of Cu(II) was calculated to be 155.51 mg/g.					
37187419	1	55	theme	biopolymer	223:232	arg1	film					258:261	all biopolymer self-assembled hydrogel film	219:261	all biopolymer self-assembled hydrogel film	219:261	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	1	55	theme	biopolymer	223:232	arg1	composite					198:206	A novel bio-based composite adsorbent	180:216	A novel bio-based composite adsorbent	180:216	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	0	56	theme	aqueous	162:168	arg1	solution					170:177	aqueous solution	162:177	aqueous solution	162:177	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.					
37187419	5	57	theme	initial	1011:1017	arg1	concentration					1025:1037	an initial metal concentration	1008:1037	an initial metal concentration of 50 mg/L	1008:1048	Using the Langmuir isotherm model for an initial metal concentration of 50 mg/L at pH 6.0 and 25 °C, the maximum adsorption of Cu(II) was calculated to be 155.51 mg/g.					
37187419	1	58	theme	self-assembled	234:247	arg1	film					258:261	all biopolymer self-assembled hydrogel film	219:261	all biopolymer self-assembled hydrogel film	219:261	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	1	58	theme	self-assembled	234:247	arg1	composite					198:206	A novel bio-based composite adsorbent	180:216	A novel bio-based composite adsorbent	180:216	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	0	59	theme	carboxymethyl	75:87	arg1	gum					94:96	carboxymethyl guar gum	75:96	carboxymethyl guar gum	75:96	An all-biopolymer self-assembling hydrogel film consisting of chitosan and carboxymethyl guar gum: A novel bio-based composite adsorbent for Cu2+ adsorption from aqueous solution.					
37187419	4	60	theme	isotherm	941:948	arg1	data					950:953	isotherm data	941:953	isotherm data	941:953	The pseudo-second-order kinetic and Langmuir isotherm models are highly correlated with the kinetic and equilibrium isotherm data, respectively.					
37187419	5	61	theme	metal	1019:1023	arg1	concentration					1025:1037	an initial metal concentration	1008:1037	an initial metal concentration of 50 mg/L	1008:1048	Using the Langmuir isotherm model for an initial metal concentration of 50 mg/L at pH 6.0 and 25 °C, the maximum adsorption of Cu(II) was calculated to be 155.51 mg/g.					
37187419	7	62	theme	CS/CMGG	1276:1282	arg1	regeneration					1293:1304	the loaded CS/CMGG hydrogel regeneration	1265:1304	the loaded CS/CMGG hydrogel regeneration	1265:1304	Five cycles of the loaded CS/CMGG hydrogel regeneration and reuse were successfully achieved without an appreciable difference in Cu(II) removal percentage.					
37187419	1	63	theme	hydrogel	249:256	arg1	film					258:261	all biopolymer self-assembled hydrogel film	219:261	all biopolymer self-assembled hydrogel film	219:261	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	1	63	theme	hydrogel	249:256	arg1	composite					198:206	A novel bio-based composite adsorbent	180:216	A novel bio-based composite adsorbent	180:216	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	2	64	theme	network	524:530	arg1	structure					532:540	the network structure	520:540	the network structure	520:540	Various analysis demonstrated the electrostatic interactions and hydrogen bondings within the network structure are responsible for gelling, crosslinking, and forming a 3D structure.					
37187419	1	65	theme	guar	342:345	arg1	CMGG					352:355	CMGG	352:355	CMGG	352:355	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	1	65	theme	guar	342:345	arg1	gum					347:349	carboxymethyl guar gum	328:349	carboxymethyl guar gum (CMGG)	328:356	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	1	66	from	biopolymers	358:368	arg1	water					373:377	water	373:377	water	373:377	A novel bio-based composite adsorbent, all biopolymer self-assembled hydrogel film has been prepared by eco-friendly amalgamating chitosan (CS) and carboxymethyl guar gum (CMGG) biopolymers in water without needing small molecules for cross-linking.					
37187419	6	67	theme	exchange	1187:1194	arg1	combination					1140:1150	A combination	1138:1150	A combination of adsorption-complexation and ion exchange	1138:1194	A combination of adsorption-complexation and ion exchange must be involved in Cu(II) adsorption on the CS/CMGG.					
37187419	2	68	theme	electrostatic	464:476	arg1	interactions					478:489	the electrostatic interactions	460:489	the electrostatic interactions	460:489	Various analysis demonstrated the electrostatic interactions and hydrogen bondings within the network structure are responsible for gelling, crosslinking, and forming a 3D structure.					
37187419	9	69	theme	metal	1596:1600	arg1	ions					1602:1605	heavy metal ions	1590:1605	heavy metal ions	1590:1605	A reusable bio-adsorbent for removing heavy metal ions was developed that is eco-friendly, sustainable, and efficient.					
35104013	7	0	theme	oligomannose-specific	1173:1193	arg1	antibodies					1195:1204	some oligomannose-specific antibodies	1168:1204	some oligomannose-specific antibodies	1168:1204	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	1	1	theme	relevant	199:206	arg1	epitopes					208:215	relevant epitopes	199:215	relevant epitopes to elicit broadly neutralizing antibodies (bnAbs)	199:265	Oligomannose-type glycans on the spike protein of HIV-1 constitute relevant epitopes to elicit broadly neutralizing antibodies (bnAbs).					
35104013	7	2	dep	observations	1069:1080	arg1	structure					1103:1111	a complete Man9 structure	1087:1111	a complete Man9 structure	1087:1111	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	7	2	dep	observations	1069:1080	arg1	motif					1158:1162	the preferred antigenic binding motif	1126:1162	the preferred antigenic binding motif for some oligomannose-specific antibodies	1126:1204	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	4	3	theme	glycosyl	666:673	arg1	donors					675:680	thiocresyl and trichloroacetimidate glycosyl donors	630:680	thiocresyl and trichloroacetimidate glycosyl donors	630:680	Chain extensions were achieved using [4+3] or [4+5] block synthesis of thiocresyl and trichloroacetimidate glycosyl donors.					
35104013	0	4	theme	HIV-1-Neutralizing	101:118	arg1	Antibodies					120:129	Oligomannose-Specific HIV-1-Neutralizing Antibodies	79:129	Oligomannose-Specific HIV-1-Neutralizing Antibodies	79:129	Synthetic Neoglycoconjugates of Hepta- and Nonamannoside Ligands for Eliciting Oligomannose-Specific HIV-1-Neutralizing Antibodies.					
35104013	7	5	theme	glycoside	1233:1241	arg1	designs					1243:1249	glycoside designs	1233:1249	glycoside designs	1233:1249	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	5	6	theme	Subsequent	683:692	arg1	deprotection					701:712	Subsequent global deprotection	683:712	Subsequent global deprotection	683:712	Subsequent global deprotection generated the 3-aminopropyl oligosaccharide ligands.					
35104013	7	7	theme	HIV-1-neutralizing	1283:1300	arg1	antibodies					1302:1311	oligomannose-targeted HIV-1-neutralizing antibodies	1261:1311	oligomannose-targeted HIV-1-neutralizing antibodies	1261:1311	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	4	8	theme	trichloroacetimidate	645:664	arg1	donors					675:680	thiocresyl and trichloroacetimidate glycosyl donors	630:680	thiocresyl and trichloroacetimidate glycosyl donors	630:680	Chain extensions were achieved using [4+3] or [4+5] block synthesis of thiocresyl and trichloroacetimidate glycosyl donors.					
35104013	7	9	theme	oligomannose-targeted	1261:1281	arg1	antibodies					1302:1311	oligomannose-targeted HIV-1-neutralizing antibodies	1261:1311	oligomannose-targeted HIV-1-neutralizing antibodies	1261:1311	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	5	10	theme	global	694:699	arg1	deprotection					701:712	Subsequent global deprotection	683:712	Subsequent global deprotection	683:712	Subsequent global deprotection generated the 3-aminopropyl oligosaccharide ligands.					
35104013	2	11	theme	hepta-	328:333	arg1	ligands					352:358	α- and β-linked hepta- and nonamannosyl ligands	312:358	α- and β-linked hepta- and nonamannosyl ligands	312:358	Herein we describe an improved synthesis of α- and β-linked hepta- and nonamannosyl ligands that were subsequently converted into BSA and CRM197 neoglycoconjugates.					
35104013	4	12	theme	Chain	559:563	arg1	extensions					565:574	Chain extensions	559:574	Chain extensions	559:574	Chain extensions were achieved using [4+3] or [4+5] block synthesis of thiocresyl and trichloroacetimidate glycosyl donors.					
35104013	2	13	theme	β-linked	319:326	arg1	ligands					352:358	α- and β-linked hepta- and nonamannosyl ligands	312:358	α- and β-linked hepta- and nonamannosyl ligands	312:358	Herein we describe an improved synthesis of α- and β-linked hepta- and nonamannosyl ligands that were subsequently converted into BSA and CRM197 neoglycoconjugates.					
35104013	7	14	theme	complete	1089:1096	arg1	motif					1158:1162	the preferred antigenic binding motif	1126:1162	the preferred antigenic binding motif for some oligomannose-specific antibodies	1126:1204	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	7	14	theme	complete	1089:1096	arg1	structure					1103:1111	a complete Man9 structure	1087:1111	a complete Man9 structure	1087:1111	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	2	15	dep	BSA	398:400	arg1	neoglycoconjugates					413:430	neoglycoconjugates	413:430	neoglycoconjugates	413:430	Herein we describe an improved synthesis of α- and β-linked hepta- and nonamannosyl ligands that were subsequently converted into BSA and CRM197 neoglycoconjugates.					
35104013	4	16	theme	donors	675:680	arg1	synthesis					617:625	synthesis	617:625	synthesis of thiocresyl and trichloroacetimidate glycosyl donors	617:680	Chain extensions were achieved using [4+3] or [4+5] block synthesis of thiocresyl and trichloroacetimidate glycosyl donors.					
35104013	6	17	theme	IgG	1022:1024	arg1	antibodies					1026:1035	the corresponding IgG antibodies	1004:1035	the corresponding IgG antibodies	1004:1035	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	6	18	theme	ligands	925:931	arg1	binding					900:906	comparable binding	889:906	comparable binding of both mannosyl ligands by Fab fragments	889:948	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	6	19	theme	nonamannosyl	826:837	arg1	conjugates					839:848	the β-anomeric hepta- and nonamannosyl conjugates	800:848	the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs	800:880	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	6	20	theme	corresponding	1008:1020	arg1	antibodies					1026:1035	the corresponding IgG antibodies	1004:1035	the corresponding IgG antibodies	1004:1035	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	3	21	theme	anomeric	463:470	arg1	glycosides					493:502	anomeric 3-azidopropyl spacer glycosides	463:502	anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors	463:556	We assembled the ligands from anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors.					
35104013	6	22	theme	mannosyl	916:923	arg1	ligands					925:931	both mannosyl ligands	911:931	both mannosyl ligands	911:931	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	2	23	theme	α-	312:313	arg1	ligands					352:358	α- and β-linked hepta- and nonamannosyl ligands	312:358	α- and β-linked hepta- and nonamannosyl ligands	312:358	Herein we describe an improved synthesis of α- and β-linked hepta- and nonamannosyl ligands that were subsequently converted into BSA and CRM197 neoglycoconjugates.					
35104013	3	24	theme	select	509:514	arg1	donors					551:556	select 3-O-protected thiocresyl mannoside donors	509:556	select 3-O-protected thiocresyl mannoside donors	509:556	We assembled the ligands from anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors.					
35104013	4	25	theme	thiocresyl	630:639	arg1	donors					675:680	thiocresyl and trichloroacetimidate glycosyl donors	630:680	thiocresyl and trichloroacetimidate glycosyl donors	630:680	Chain extensions were achieved using [4+3] or [4+5] block synthesis of thiocresyl and trichloroacetimidate glycosyl donors.					
35104013	5	26	theme	oligosaccharide	742:756	arg1	ligands					758:764	the 3-aminopropyl oligosaccharide ligands	724:764	the 3-aminopropyl oligosaccharide ligands	724:764	Subsequent global deprotection generated the 3-aminopropyl oligosaccharide ligands.					
35104013	6	27	theme	hepta-	815:820	arg1	conjugates					839:848	the β-anomeric hepta- and nonamannosyl conjugates	800:848	the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs	800:880	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	3	28	theme	spacer	486:491	arg1	glycosides					493:502	anomeric 3-azidopropyl spacer glycosides	463:502	anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors	463:556	We assembled the ligands from anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors.					
35104013	0	29	theme	Synthetic	0:8	arg1	Neoglycoconjugates					10:27	Synthetic Neoglycoconjugates	0:27	Synthetic Neoglycoconjugates of Hepta- and Nonamannoside Ligands for Eliciting Oligomannose-Specific HIV-1-Neutralizing Antibodies	0:129	Synthetic Neoglycoconjugates of Hepta- and Nonamannoside Ligands for Eliciting Oligomannose-Specific HIV-1-Neutralizing Antibodies.					
35104013	6	30	theme	Fab	936:938	arg1	fragments					940:948	Fab fragments	936:948	Fab fragments	936:948	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	7	31	theme	previous	1060:1067	arg1	observations					1069:1080	previous observations	1060:1080	previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies	1060:1204	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	1	32	theme	Oligomannose-type	132:148	arg1	glycans					150:156	Oligomannose-type glycans	132:156	Oligomannose-type glycans on the spike protein of HIV-1	132:186	Oligomannose-type glycans on the spike protein of HIV-1 constitute relevant epitopes to elicit broadly neutralizing antibodies (bnAbs).					
35104013	6	33	theme	β-anomeric	804:813	arg1	conjugates					839:848	the β-anomeric hepta- and nonamannosyl conjugates	800:848	the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs	800:880	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	1	34	theme	neutralizing	235:246	arg1	bnAbs					260:264	bnAbs	260:264	bnAbs	260:264	Oligomannose-type glycans on the spike protein of HIV-1 constitute relevant epitopes to elicit broadly neutralizing antibodies (bnAbs).					
35104013	1	34	theme	neutralizing	235:246	arg1	antibodies					248:257	broadly neutralizing antibodies	227:257	broadly neutralizing antibodies (bnAbs)	227:265	Oligomannose-type glycans on the spike protein of HIV-1 constitute relevant epitopes to elicit broadly neutralizing antibodies (bnAbs).					
35104013	0	35	theme	Hepta-	32:37	arg1	Ligands					57:63	Hepta- and Nonamannoside Ligands	32:63	Hepta- and Nonamannoside Ligands	32:63	Synthetic Neoglycoconjugates of Hepta- and Nonamannoside Ligands for Eliciting Oligomannose-Specific HIV-1-Neutralizing Antibodies.					
35104013	7	36	theme	Man9	1098:1101	arg1	motif					1158:1162	the preferred antigenic binding motif	1126:1162	the preferred antigenic binding motif for some oligomannose-specific antibodies	1126:1204	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	7	36	theme	Man9	1098:1101	arg1	structure					1103:1111	a complete Man9 structure	1087:1111	a complete Man9 structure	1087:1111	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	2	37	theme	nonamannosyl	339:350	arg1	ligands					352:358	α- and β-linked hepta- and nonamannosyl ligands	312:358	α- and β-linked hepta- and nonamannosyl ligands	312:358	Herein we describe an improved synthesis of α- and β-linked hepta- and nonamannosyl ligands that were subsequently converted into BSA and CRM197 neoglycoconjugates.					
35104013	3	38	theme	mannoside	541:549	arg1	donors					551:556	select 3-O-protected thiocresyl mannoside donors	509:556	select 3-O-protected thiocresyl mannoside donors	509:556	We assembled the ligands from anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors.					
35104013	2	39	theme	ligands	352:358	arg1	synthesis					299:307	an improved synthesis	287:307	an improved synthesis of α- and β-linked hepta- and nonamannosyl ligands that were subsequently converted into BSA and CRM197 neoglycoconjugates	287:430	Herein we describe an improved synthesis of α- and β-linked hepta- and nonamannosyl ligands that were subsequently converted into BSA and CRM197 neoglycoconjugates.					
35104013	7	40	theme	antigenic	1140:1148	arg1	structure					1103:1111	a complete Man9 structure	1087:1111	a complete Man9 structure	1087:1111	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	7	40	theme	antigenic	1140:1148	arg1	motif					1158:1162	the preferred antigenic binding motif	1126:1162	the preferred antigenic binding motif for some oligomannose-specific antibodies	1126:1204	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	7	41	contain	have	1211:1214	arg2	implications					1216:1227	implications	1216:1227	implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies	1216:1311	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	7	41	contain	have	1211:1214	arg1	results					1044:1050	These results	1038:1050	These results	1038:1050	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	0	42	theme	Nonamannoside	43:55	arg1	Ligands					57:63	Hepta- and Nonamannoside Ligands	32:63	Hepta- and Nonamannoside Ligands	32:63	Synthetic Neoglycoconjugates of Hepta- and Nonamannoside Ligands for Eliciting Oligomannose-Specific HIV-1-Neutralizing Antibodies.					
35104013	2	43	link	β-linked	319:326	arg1	ligands					352:358	α- and β-linked hepta- and nonamannosyl ligands	312:358	α- and β-linked hepta- and nonamannosyl ligands	312:358	Herein we describe an improved synthesis of α- and β-linked hepta- and nonamannosyl ligands that were subsequently converted into BSA and CRM197 neoglycoconjugates.					
35104013	6	44	with	conjugates	839:848	arg1	selection					857:865	a selection	855:865	a selection of HIV-1 bnAbs	855:880	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	6	45	theme	conjugate	991:999	arg1	binding					961:967	lesser binding	954:967	lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies	954:1035	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	3	46	theme	3-O-protected	516:528	arg1	donors					551:556	select 3-O-protected thiocresyl mannoside donors	509:556	select 3-O-protected thiocresyl mannoside donors	509:556	We assembled the ligands from anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors.					
35104013	3	47	theme	3-azidopropyl	472:484	arg1	glycosides					493:502	anomeric 3-azidopropyl spacer glycosides	463:502	anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors	463:556	We assembled the ligands from anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors.					
35104013	6	48	theme	nonasaccharide	976:989	arg1	conjugate					991:999	the nonasaccharide conjugate	972:999	the nonasaccharide conjugate	972:999	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	6	49	theme	comparable	889:898	arg1	binding					900:906	comparable binding	889:906	comparable binding of both mannosyl ligands by Fab fragments	889:948	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	3	50	theme	thiocresyl	530:539	arg1	donors					551:556	select 3-O-protected thiocresyl mannoside donors	509:556	select 3-O-protected thiocresyl mannoside donors	509:556	We assembled the ligands from anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors.					
35104013	0	51	theme	Ligands	57:63	arg1	Neoglycoconjugates					10:27	Synthetic Neoglycoconjugates	0:27	Synthetic Neoglycoconjugates of Hepta- and Nonamannoside Ligands for Eliciting Oligomannose-Specific HIV-1-Neutralizing Antibodies	0:129	Synthetic Neoglycoconjugates of Hepta- and Nonamannoside Ligands for Eliciting Oligomannose-Specific HIV-1-Neutralizing Antibodies.					
35104013	5	52	theme	3-aminopropyl	728:740	arg1	ligands					758:764	the 3-aminopropyl oligosaccharide ligands	724:764	the 3-aminopropyl oligosaccharide ligands	724:764	Subsequent global deprotection generated the 3-aminopropyl oligosaccharide ligands.					
35104013	6	53	theme	bnAbs	876:880	arg1	selection					857:865	a selection	855:865	a selection of HIV-1 bnAbs	855:880	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	1	54	theme	spike	165:169	arg1	protein					171:177	the spike protein	161:177	the spike protein of HIV-1	161:186	Oligomannose-type glycans on the spike protein of HIV-1 constitute relevant epitopes to elicit broadly neutralizing antibodies (bnAbs).					
35104013	1	55	from	glycans	150:156	arg1	protein					171:177	the spike protein	161:177	the spike protein of HIV-1	161:186	Oligomannose-type glycans on the spike protein of HIV-1 constitute relevant epitopes to elicit broadly neutralizing antibodies (bnAbs).					
35104013	3	56	from	donors	551:556	arg1	glycosides					493:502	anomeric 3-azidopropyl spacer glycosides	463:502	anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors	463:556	We assembled the ligands from anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors.					
35104013	6	57	theme	binding	773:779	arg1	data					781:784	ELISA binding data	767:784	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs	767:880	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	6	58	theme	HIV-1	870:874	arg1	bnAbs					876:880	HIV-1 bnAbs	870:880	HIV-1 bnAbs	870:880	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	6	59	theme	lesser	954:959	arg1	binding					961:967	lesser binding	954:967	lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies	954:1035	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	6	60	theme	ELISA	767:771	arg1	data					781:784	ELISA binding data	767:784	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs	767:880	ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both mannosyl ligands by Fab fragments yet lesser binding of the nonasaccharide conjugate by the corresponding IgG antibodies.					
35104013	0	61	theme	Oligomannose-Specific	79:99	arg1	Antibodies					120:129	Oligomannose-Specific HIV-1-Neutralizing Antibodies	79:129	Oligomannose-Specific HIV-1-Neutralizing Antibodies	79:129	Synthetic Neoglycoconjugates of Hepta- and Nonamannoside Ligands for Eliciting Oligomannose-Specific HIV-1-Neutralizing Antibodies.					
35104013	7	62	theme	binding	1150:1156	arg1	structure					1103:1111	a complete Man9 structure	1087:1111	a complete Man9 structure	1087:1111	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	7	62	theme	binding	1150:1156	arg1	motif					1158:1162	the preferred antigenic binding motif	1126:1162	the preferred antigenic binding motif for some oligomannose-specific antibodies	1126:1204	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	2	63	theme	improved	290:297	arg1	synthesis					299:307	an improved synthesis	287:307	an improved synthesis of α- and β-linked hepta- and nonamannosyl ligands that were subsequently converted into BSA and CRM197 neoglycoconjugates	287:430	Herein we describe an improved synthesis of α- and β-linked hepta- and nonamannosyl ligands that were subsequently converted into BSA and CRM197 neoglycoconjugates.					
35104013	7	64	theme	preferred	1130:1138	arg1	structure					1103:1111	a complete Man9 structure	1087:1111	a complete Man9 structure	1087:1111	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	7	64	theme	preferred	1130:1138	arg1	motif					1158:1162	the preferred antigenic binding motif	1126:1162	the preferred antigenic binding motif for some oligomannose-specific antibodies	1126:1204	These results support previous observations that a complete Man9 structure might not be the preferred antigenic binding motif for some oligomannose-specific antibodies, and have implications for glycoside designs to elicit oligomannose-targeted HIV-1-neutralizing antibodies.					
35104013	1	65	theme	HIV-1	182:186	arg1	protein					171:177	the spike protein	161:177	the spike protein of HIV-1	161:186	Oligomannose-type glycans on the spike protein of HIV-1 constitute relevant epitopes to elicit broadly neutralizing antibodies (bnAbs).					
35104013	4	66	dep	block	611:615	arg1	[4+5					605:608	[4+5	605:608	[4+5	605:608	Chain extensions were achieved using [4+3] or [4+5] block synthesis of thiocresyl and trichloroacetimidate glycosyl donors.					
35200626	8	0	theme	intestinal	1182:1191	arg1	composition					1204:1214	intestinal microbiota composition	1182:1214	intestinal microbiota composition	1182:1214	COS administration could optimize intestinal microbiota composition by increasing the abundance of norank_f_Muribaculaceae, Lactobacillus and Alistipes, while decreasing the abundance of Turicibacte.					
35200626	2	1	theme	sulfate-induced	366:380	arg1	colitis					382:388	dextran sodium sulfate-induced colitis	351:388	dextran sodium sulfate-induced colitis in mice	351:396	In this study, COS were found to significantly attenuate dextran sodium sulfate-induced colitis in mice by decreasing disease activity index scores, downregulating pro-inflammatory cytokines, and upregulating Mucin-2 levels.					
35200626	8	2	theme	norank_f_Muribaculaceae	1247:1269	arg1	abundance					1234:1242	the abundance	1230:1242	the abundance of norank_f_Muribaculaceae, Lactobacillus and Alistipes	1230:1298	COS administration could optimize intestinal microbiota composition by increasing the abundance of norank_f_Muribaculaceae, Lactobacillus and Alistipes, while decreasing the abundance of Turicibacte.					
35200626	0	3	theme	NF-κB	105:109	arg1	Pathway					111:117	PPARγ/SIRT1-Mediated NF-κB Pathway	84:117	PPARγ/SIRT1-Mediated NF-κB Pathway	84:117	Chitosan Oligosaccharides Alleviate Colitis by Regulating Intestinal Microbiota and PPARγ/SIRT1-Mediated NF-κB Pathway.					
35200626	10	4	theme	NF-κB	1522:1526	arg1	pathway					1528:1534	the PPARγ/SIRT1-mediated NF-κB pathway	1497:1534	the PPARγ/SIRT1-mediated NF-κB pathway	1497:1534	Overall, COS can improve colitis by regulating intestinal microbiota and the PPARγ/SIRT1-mediated NF-κB pathway.					
35200626	2	5	theme	sodium	359:364	arg1	colitis					382:388	dextran sodium sulfate-induced colitis	351:388	dextran sodium sulfate-induced colitis in mice	351:396	In this study, COS were found to significantly attenuate dextran sodium sulfate-induced colitis in mice by decreasing disease activity index scores, downregulating pro-inflammatory cytokines, and upregulating Mucin-2 levels.					
35200626	7	6	theme	Firmicutes/Bacteroidetes	1116:1139	arg1	ratio					1141:1145	the Firmicutes/Bacteroidetes ratio	1112:1145	the Firmicutes/Bacteroidetes ratio	1112:1145	Moreover, COS treatment increased the diversity of intestinal microbiota and partly restored the Firmicutes/Bacteroidetes ratio.					
35200626	4	7	theme	IL-6	787:790	arg1	production					766:775	the production	762:775	the production of NO and IL-6	762:790	Importantly, COS inhibited the activation of the NF-κB signaling pathway via activating PPARγ and SIRT1, thus reducing the production of NO and IL-6.					
35200626	4	8	theme	NF-κB	692:696	arg1	pathway					708:714	the NF-κB signaling pathway	688:714	the NF-κB signaling pathway	688:714	Importantly, COS inhibited the activation of the NF-κB signaling pathway via activating PPARγ and SIRT1, thus reducing the production of NO and IL-6.					
35200626	6	9	theme	silencing	993:1001	arg1	SIRT1					1003:1007	silencing SIRT1	993:1007	silencing SIRT1	993:1007	COS also activated SIRT1 to reduce the acetylation of p65 protein at lysine 310, which was reversed by silencing SIRT1 by siRNA.					
35200626	8	10	theme	microbiota	1193:1202	arg1	composition					1204:1214	intestinal microbiota composition	1182:1214	intestinal microbiota composition	1182:1214	COS administration could optimize intestinal microbiota composition by increasing the abundance of norank_f_Muribaculaceae, Lactobacillus and Alistipes, while decreasing the abundance of Turicibacte.					
35200626	3	11	from	levels	556:561	arg1	cells					636:640	lipopolysaccharide-stimulated RAW 264.7 cells	596:640	lipopolysaccharide-stimulated RAW 264.7 cells	596:640	COS also significantly inhibited the levels of nitric oxide (NO) and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells.					
35200626	1	12	contain	have	171:174	arg1	COS					147:149	COS	147:149	COS	147:149	Chitosan oligosaccharides (COS) have been shown to have potential protective effects against colitis, but the mechanism underlying this effect has not been fully elucidated.					
35200626	1	12	contain	have	171:174	arg1	oligosaccharides					129:144	Chitosan oligosaccharides	120:144	Chitosan oligosaccharides (COS)	120:150	Chitosan oligosaccharides (COS) have been shown to have potential protective effects against colitis, but the mechanism underlying this effect has not been fully elucidated.					
35200626	1	12	contain	have	171:174	arg2	effects					197:203	potential protective effects	176:203	potential protective effects	176:203	Chitosan oligosaccharides (COS) have been shown to have potential protective effects against colitis, but the mechanism underlying this effect has not been fully elucidated.					
35200626	3	13	theme	nitric	566:571	arg1	NO					580:581	NO	580:581	NO	580:581	COS also significantly inhibited the levels of nitric oxide (NO) and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells.					
35200626	3	13	theme	nitric	566:571	arg1	oxide					573:577	nitric oxide	566:577	nitric oxide (NO)	566:582	COS also significantly inhibited the levels of nitric oxide (NO) and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells.					
35200626	1	14	theme	protective	186:195	arg1	effects					197:203	potential protective effects	176:203	potential protective effects	176:203	Chitosan oligosaccharides (COS) have been shown to have potential protective effects against colitis, but the mechanism underlying this effect has not been fully elucidated.					
35200626	3	15	theme	oxide	573:577	arg1	levels					556:561	the levels	552:561	the levels of nitric oxide (NO) and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells	552:640	COS also significantly inhibited the levels of nitric oxide (NO) and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells.					
35200626	3	16	theme	IL-6	588:591	arg1	levels					556:561	the levels	552:561	the levels of nitric oxide (NO) and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells	552:640	COS also significantly inhibited the levels of nitric oxide (NO) and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells.					
35200626	5	17	from	effects	853:859	arg1	cells					883:887	LPS-treated cells	871:887	LPS-treated cells	871:887	The antagonist of PPARγ could abolish the anti-inflammatory effects of COS in LPS-treated cells.					
35200626	4	18	theme	pathway	708:714	arg1	activation					674:683	the activation	670:683	the activation of the NF-κB signaling pathway	670:714	Importantly, COS inhibited the activation of the NF-κB signaling pathway via activating PPARγ and SIRT1, thus reducing the production of NO and IL-6.					
35200626	10	19	theme	intestinal	1471:1480	arg1	microbiota					1482:1491	intestinal microbiota	1471:1491	intestinal microbiota	1471:1491	Overall, COS can improve colitis by regulating intestinal microbiota and the PPARγ/SIRT1-mediated NF-κB pathway.					
35200626	1	20	theme	Chitosan	120:127	arg1	COS					147:149	COS	147:149	COS	147:149	Chitosan oligosaccharides (COS) have been shown to have potential protective effects against colitis, but the mechanism underlying this effect has not been fully elucidated.					
35200626	1	20	theme	Chitosan	120:127	arg1	oligosaccharides					129:144	Chitosan oligosaccharides	120:144	Chitosan oligosaccharides (COS)	120:150	Chitosan oligosaccharides (COS) have been shown to have potential protective effects against colitis, but the mechanism underlying this effect has not been fully elucidated.					
35200626	4	21	theme	signaling	698:706	arg1	pathway					708:714	the NF-κB signaling pathway	688:714	the NF-κB signaling pathway	688:714	Importantly, COS inhibited the activation of the NF-κB signaling pathway via activating PPARγ and SIRT1, thus reducing the production of NO and IL-6.					
35200626	2	22	theme	pro-inflammatory	458:473	arg1	cytokines					475:483	pro-inflammatory cytokines	458:483	pro-inflammatory cytokines	458:483	In this study, COS were found to significantly attenuate dextran sodium sulfate-induced colitis in mice by decreasing disease activity index scores, downregulating pro-inflammatory cytokines, and upregulating Mucin-2 levels.					
35200626	2	23	located	found	318:322	arg2	COS					309:311	COS	309:311	COS	309:311	In this study, COS were found to significantly attenuate dextran sodium sulfate-induced colitis in mice by decreasing disease activity index scores, downregulating pro-inflammatory cytokines, and upregulating Mucin-2 levels.					
35200626	2	23	located	found	318:322	arg1	study					302:306	this study	297:306	this study	297:306	In this study, COS were found to significantly attenuate dextran sodium sulfate-induced colitis in mice by decreasing disease activity index scores, downregulating pro-inflammatory cytokines, and upregulating Mucin-2 levels.					
35200626	7	24	theme	intestinal	1070:1079	arg1	microbiota					1081:1090	intestinal microbiota	1070:1090	intestinal microbiota	1070:1090	Moreover, COS treatment increased the diversity of intestinal microbiota and partly restored the Firmicutes/Bacteroidetes ratio.					
35200626	2	25	from	colitis	382:388	arg1	mice					393:396	mice	393:396	mice	393:396	In this study, COS were found to significantly attenuate dextran sodium sulfate-induced colitis in mice by decreasing disease activity index scores, downregulating pro-inflammatory cytokines, and upregulating Mucin-2 levels.					
35200626	4	26	theme	NO	780:781	arg1	production					766:775	the production	762:775	the production of NO and IL-6	762:790	Importantly, COS inhibited the activation of the NF-κB signaling pathway via activating PPARγ and SIRT1, thus reducing the production of NO and IL-6.					
35200626	0	27	theme	Alleviate	26:34	arg1	Colitis					36:42	Alleviate Colitis	26:42	Alleviate Colitis	26:42	Chitosan Oligosaccharides Alleviate Colitis by Regulating Intestinal Microbiota and PPARγ/SIRT1-Mediated NF-κB Pathway.					
35200626	7	28	theme	microbiota	1081:1090	arg1	diversity					1057:1065	the diversity	1053:1065	the diversity of intestinal microbiota	1053:1090	Moreover, COS treatment increased the diversity of intestinal microbiota and partly restored the Firmicutes/Bacteroidetes ratio.					
35200626	1	29	theme	potential	176:184	arg1	effects					197:203	potential protective effects	176:203	potential protective effects	176:203	Chitosan oligosaccharides (COS) have been shown to have potential protective effects against colitis, but the mechanism underlying this effect has not been fully elucidated.					
35200626	5	30	theme	COS	864:866	arg1	effects					853:859	the anti-inflammatory effects	831:859	the anti-inflammatory effects of COS in LPS-treated cells	831:887	The antagonist of PPARγ could abolish the anti-inflammatory effects of COS in LPS-treated cells.					
35200626	8	31	theme	Lactobacillus	1272:1284	arg1	abundance					1234:1242	the abundance	1230:1242	the abundance of norank_f_Muribaculaceae, Lactobacillus and Alistipes	1230:1298	COS administration could optimize intestinal microbiota composition by increasing the abundance of norank_f_Muribaculaceae, Lactobacillus and Alistipes, while decreasing the abundance of Turicibacte.					
35200626	9	32	theme	butyrate	1414:1421	arg1	levels					1389:1394	the levels	1385:1394	the levels of propionate and butyrate	1385:1421	Furthermore, COS could also increase the levels of propionate and butyrate.					
35200626	2	33	theme	index	429:433	arg1	scores					435:440	disease activity index scores	412:440	disease activity index scores	412:440	In this study, COS were found to significantly attenuate dextran sodium sulfate-induced colitis in mice by decreasing disease activity index scores, downregulating pro-inflammatory cytokines, and upregulating Mucin-2 levels.					
35200626	2	34	theme	Mucin-2	503:509	arg1	levels					511:516	Mucin-2 levels	503:516	Mucin-2 levels	503:516	In this study, COS were found to significantly attenuate dextran sodium sulfate-induced colitis in mice by decreasing disease activity index scores, downregulating pro-inflammatory cytokines, and upregulating Mucin-2 levels.					
35200626	5	35	theme	anti-inflammatory	835:851	arg1	effects					853:859	the anti-inflammatory effects	831:859	the anti-inflammatory effects of COS in LPS-treated cells	831:887	The antagonist of PPARγ could abolish the anti-inflammatory effects of COS in LPS-treated cells.					
35200626	2	36	theme	activity	420:427	arg1	scores					435:440	disease activity index scores	412:440	disease activity index scores	412:440	In this study, COS were found to significantly attenuate dextran sodium sulfate-induced colitis in mice by decreasing disease activity index scores, downregulating pro-inflammatory cytokines, and upregulating Mucin-2 levels.					
35200626	5	37	theme	PPARγ	811:815	arg1	antagonist					797:806	The antagonist	793:806	The antagonist of PPARγ	793:815	The antagonist of PPARγ could abolish the anti-inflammatory effects of COS in LPS-treated cells.					
35200626	9	38	theme	propionate	1399:1408	arg1	levels					1389:1394	the levels	1385:1394	the levels of propionate and butyrate	1385:1421	Furthermore, COS could also increase the levels of propionate and butyrate.					
35200626	3	39	theme	lipopolysaccharide-stimulated	596:624	arg1	cells					636:640	lipopolysaccharide-stimulated RAW 264.7 cells	596:640	lipopolysaccharide-stimulated RAW 264.7 cells	596:640	COS also significantly inhibited the levels of nitric oxide (NO) and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells.					
35200626	8	40	theme	COS	1148:1150	arg1	administration					1152:1165	COS administration	1148:1165	COS administration	1148:1165	COS administration could optimize intestinal microbiota composition by increasing the abundance of norank_f_Muribaculaceae, Lactobacillus and Alistipes, while decreasing the abundance of Turicibacte.					
35200626	2	41	theme	dextran	351:357	arg1	sodium					359:364	dextran sodium	351:364	dextran sodium sulfate-induced colitis in mice	351:396	In this study, COS were found to significantly attenuate dextran sodium sulfate-induced colitis in mice by decreasing disease activity index scores, downregulating pro-inflammatory cytokines, and upregulating Mucin-2 levels.					
35200626	0	42	theme	Intestinal	58:67	arg1	Microbiota					69:78	Intestinal Microbiota	58:78	Intestinal Microbiota	58:78	Chitosan Oligosaccharides Alleviate Colitis by Regulating Intestinal Microbiota and PPARγ/SIRT1-Mediated NF-κB Pathway.					
35200626	2	43	theme	disease	412:418	arg1	scores					435:440	disease activity index scores	412:440	disease activity index scores	412:440	In this study, COS were found to significantly attenuate dextran sodium sulfate-induced colitis in mice by decreasing disease activity index scores, downregulating pro-inflammatory cytokines, and upregulating Mucin-2 levels.					
35200626	3	44	theme	RAW	626:628	arg1	cells					636:640	lipopolysaccharide-stimulated RAW 264.7 cells	596:640	lipopolysaccharide-stimulated RAW 264.7 cells	596:640	COS also significantly inhibited the levels of nitric oxide (NO) and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells.					
35200626	10	45	theme	PPARγ/SIRT1-mediated	1501:1520	arg1	pathway					1528:1534	the PPARγ/SIRT1-mediated NF-κB pathway	1497:1534	the PPARγ/SIRT1-mediated NF-κB pathway	1497:1534	Overall, COS can improve colitis by regulating intestinal microbiota and the PPARγ/SIRT1-mediated NF-κB pathway.					
35200626	6	46	theme	protein	948:954	arg1	acetylation					929:939	the acetylation	925:939	the acetylation of p65 protein at lysine 310	925:968	COS also activated SIRT1 to reduce the acetylation of p65 protein at lysine 310, which was reversed by silencing SIRT1 by siRNA.					
35200626	7	47	theme	COS	1029:1031	arg1	treatment					1033:1041	COS treatment	1029:1041	COS treatment	1029:1041	Moreover, COS treatment increased the diversity of intestinal microbiota and partly restored the Firmicutes/Bacteroidetes ratio.					
35200626	8	48	theme	Turicibacte	1335:1345	arg1	abundance					1322:1330	the abundance	1318:1330	the abundance of Turicibacte	1318:1345	COS administration could optimize intestinal microbiota composition by increasing the abundance of norank_f_Muribaculaceae, Lactobacillus and Alistipes, while decreasing the abundance of Turicibacte.					
35200626	6	49	from	lysine	959:964	arg1	acetylation					929:939	the acetylation	925:939	the acetylation of p65 protein at lysine 310	925:968	COS also activated SIRT1 to reduce the acetylation of p65 protein at lysine 310, which was reversed by silencing SIRT1 by siRNA.					
35200626	0	50	theme	PPARγ/SIRT1-Mediated	84:103	arg1	Pathway					111:117	PPARγ/SIRT1-Mediated NF-κB Pathway	84:117	PPARγ/SIRT1-Mediated NF-κB Pathway	84:117	Chitosan Oligosaccharides Alleviate Colitis by Regulating Intestinal Microbiota and PPARγ/SIRT1-Mediated NF-κB Pathway.					
35200626	5	51	theme	LPS-treated	871:881	arg1	cells					883:887	LPS-treated cells	871:887	LPS-treated cells	871:887	The antagonist of PPARγ could abolish the anti-inflammatory effects of COS in LPS-treated cells.					
35200626	8	52	theme	Alistipes	1290:1298	arg1	abundance					1234:1242	the abundance	1230:1242	the abundance of norank_f_Muribaculaceae, Lactobacillus and Alistipes	1230:1298	COS administration could optimize intestinal microbiota composition by increasing the abundance of norank_f_Muribaculaceae, Lactobacillus and Alistipes, while decreasing the abundance of Turicibacte.					
35200626	6	53	theme	p65	944:946	arg1	protein					948:954	p65 protein	944:954	p65 protein	944:954	COS also activated SIRT1 to reduce the acetylation of p65 protein at lysine 310, which was reversed by silencing SIRT1 by siRNA.					
35682591	0	0	theme	High-Fat	74:81	arg1	Mice					102:105	High-Fat Diet-Induced Obese Mice	74:105	High-Fat Diet-Induced Obese Mice	74:105	High-Amylose Corn Starch Regulated Gut Microbiota and Serum Bile Acids in High-Fat Diet-Induced Obese Mice.					
35682591	2	1	theme	circulating	332:342	arg1	acids					349:353	circulating bile acids	332:353	circulating bile acids	332:353	Recently, the gut microbiota has been reported to improve metabolic health by altering circulating bile acids.					
35682591	1	2	theme	gut	229:231	arg1	microbiota					233:242	gut microbiota	229:242	gut microbiota	229:242	High-amylose corn starch is well known for its anti-obesity activity, which is mainly based on the regulatory effects on gut microbiota.					
35682591	3	3	theme	serum	472:476	arg1	acids					483:487	serum bile acids	472:487	serum bile acids	472:487	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	6	4	theme	obvious	940:946	arg1	changes					948:954	more obvious changes	935:954	more obvious changes in serum bile acids profiles	935:983	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	0	5	theme	Obese	96:100	arg1	Mice					102:105	High-Fat Diet-Induced Obese Mice	74:105	High-Fat Diet-Induced Obese Mice	74:105	High-Amylose Corn Starch Regulated Gut Microbiota and Serum Bile Acids in High-Fat Diet-Induced Obese Mice.					
35682591	4	6	dep	HACS	567:570	arg1	reduced					582:588	reduced	582:588	reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy	582:672	The results demonstrated HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles.					
35682591	4	7	theme	lipid	700:704	arg1	profiles					706:713	improved blood lipid profiles	685:713	HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles	567:713	The results demonstrated HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles.					
35682591	4	8	theme	body	602:605	arg1	gain					614:617	HFD-induced body weight gain	590:617	HFD-induced body weight gain	590:617	The results demonstrated HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles.					
35682591	0	9	theme	Diet-Induced	83:94	arg1	Mice					102:105	High-Fat Diet-Induced Obese Mice	74:105	High-Fat Diet-Induced Obese Mice	74:105	High-Amylose Corn Starch Regulated Gut Microbiota and Serum Bile Acids in High-Fat Diet-Induced Obese Mice.					
35682591	6	10	theme	acid	1094:1097	arg1	composition					1099:1109	bile acid composition	1089:1109	bile acid composition in blood	1089:1118	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	4	11	theme	blood	694:698	arg1	profiles					706:713	improved blood lipid profiles	685:713	HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles	567:713	The results demonstrated HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles.					
35682591	2	12	theme	gut	259:261	arg1	microbiota					263:272	the gut microbiota	255:272	the gut microbiota	255:272	Recently, the gut microbiota has been reported to improve metabolic health by altering circulating bile acids.					
35682591	4	13	theme	adipocyte	652:660	arg1	hypertrophy					662:672	adipocyte hypertrophy	652:672	adipocyte hypertrophy	652:672	The results demonstrated HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles.					
35682591	1	14	theme	High-amylose	108:119	arg1	starch					126:131	High-amylose corn starch	108:131	High-amylose corn starch	108:131	High-amylose corn starch is well known for its anti-obesity activity, which is mainly based on the regulatory effects on gut microbiota.					
35682591	6	15	theme	HACS	1016:1019	arg1	intervention					1021:1032	the HACS intervention	1012:1032	the HACS intervention	1012:1032	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	7	16	theme	gut	1257:1259	arg1	microbiota					1261:1270	gut microbiota	1257:1270	gut microbiota	1257:1270	In summary, our results suggested that the underlying mechanisms of anti-obesity activity of HACS may involve its regulatory effects on gut microbiota and circulating bile acids.					
35682591	1	17	theme	anti-obesity	155:166	arg1	activity					168:175	its anti-obesity activity	151:175	its anti-obesity activity	151:175	High-amylose corn starch is well known for its anti-obesity activity, which is mainly based on the regulatory effects on gut microbiota.					
35682591	6	18	theme	bile	965:968	arg1	acids					970:974	serum bile acids	959:974	serum bile acids profiles	959:983	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	3	19	theme	corn	412:415	arg1	HACS					425:428	HACS	425:428	HACS	425:428	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	3	19	theme	corn	412:415	arg1	starch					417:422	high-amylose corn starch	399:422	high-amylose corn starch (HACS)	399:429	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	0	20	theme	Corn	13:16	arg1	Starch					18:23	High-Amylose Corn Starch	0:23	High-Amylose Corn Starch	0:23	High-Amylose Corn Starch Regulated Gut Microbiota and Serum Bile Acids in High-Fat Diet-Induced Obese Mice.					
35682591	6	21	from	changes	948:954	arg1	profiles					976:983	serum bile acids profiles	959:983	serum bile acids profiles	959:983	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	7	22	theme	activity	1202:1209	arg1	mechanisms					1175:1184	the underlying mechanisms	1160:1184	the underlying mechanisms of anti-obesity activity of HACS	1160:1217	In summary, our results suggested that the underlying mechanisms of anti-obesity activity of HACS may involve its regulatory effects on gut microbiota and circulating bile acids.					
35682591	7	23	theme	HACS	1214:1217	arg1	activity					1202:1209	anti-obesity activity	1189:1209	anti-obesity activity of HACS	1189:1217	In summary, our results suggested that the underlying mechanisms of anti-obesity activity of HACS may involve its regulatory effects on gut microbiota and circulating bile acids.					
35682591	5	24	theme	Bacteroidetes	812:824	arg1	abundance					835:843	Bacteroidetes relative abundance	812:843	Bacteroidetes relative abundance being observed	812:858	Moreover, HACS also greatly impacted the gut microbiota with increased Firmicutes and decreased Bacteroidetes relative abundance being observed.					
35682591	0	25	theme	High-Amylose	0:11	arg1	Starch					18:23	High-Amylose Corn Starch	0:23	High-Amylose Corn Starch	0:23	High-Amylose Corn Starch Regulated Gut Microbiota and Serum Bile Acids in High-Fat Diet-Induced Obese Mice.					
35682591	3	26	theme	high	521:524	arg1	HFD					536:538	HFD	536:538	HFD	536:538	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	3	26	theme	high	521:524	arg1	diet					530:533	a high fat diet	519:533	a high fat diet (HFD)	519:539	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	4	27	theme	weight	607:612	arg1	gain					614:617	HFD-induced body weight gain	590:617	HFD-induced body weight gain	590:617	The results demonstrated HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles.					
35682591	6	28	theme	HFD	913:915	arg1	feeding					917:923	HFD feeding	913:923	HFD feeding	913:923	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	7	29	theme	anti-obesity	1189:1200	arg1	activity					1202:1209	anti-obesity activity	1189:1209	anti-obesity activity of HACS	1189:1217	In summary, our results suggested that the underlying mechanisms of anti-obesity activity of HACS may involve its regulatory effects on gut microbiota and circulating bile acids.					
35682591	4	30	theme	improved	685:692	arg1	profiles					706:713	improved blood lipid profiles	685:713	HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles	567:713	The results demonstrated HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles.					
35682591	6	31	theme	HFD-fed	994:1000	arg1	mice					1002:1005	the HFD-fed mice	990:1005	the HFD-fed mice with the HACS intervention	990:1032	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	5	32	theme	gut	757:759	arg1	microbiota					761:770	the gut microbiota	753:770	the gut microbiota with increased Firmicutes	753:796	Moreover, HACS also greatly impacted the gut microbiota with increased Firmicutes and decreased Bacteroidetes relative abundance being observed.					
35682591	7	33	from	effects	1246:1252	arg1	microbiota					1261:1270	gut microbiota	1257:1270	gut microbiota	1257:1270	In summary, our results suggested that the underlying mechanisms of anti-obesity activity of HACS may involve its regulatory effects on gut microbiota and circulating bile acids.					
35682591	7	33	from	effects	1246:1252	arg1	acids					1293:1297	circulating bile acids	1276:1297	circulating bile acids	1276:1297	In summary, our results suggested that the underlying mechanisms of anti-obesity activity of HACS may involve its regulatory effects on gut microbiota and circulating bile acids.					
35682591	4	34	theme	lipid	628:632	arg1	accumulation					634:645	hepatic lipid accumulation	620:645	hepatic lipid accumulation	620:645	The results demonstrated HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles.					
35682591	6	35	from	composition	1099:1109	arg1	blood					1114:1118	blood	1114:1118	blood	1114:1118	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	4	36	theme	hepatic	620:626	arg1	accumulation					634:645	hepatic lipid accumulation	620:645	hepatic lipid accumulation	620:645	The results demonstrated HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles.					
35682591	6	37	theme	HFD-induced	1062:1072	arg1	alterations					1074:1084	HFD-induced alterations	1062:1084	HFD-induced alterations	1062:1084	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	5	38	with	microbiota	761:770	arg1	Firmicutes					787:796	increased Firmicutes	777:796	increased Firmicutes	777:796	Moreover, HACS also greatly impacted the gut microbiota with increased Firmicutes and decreased Bacteroidetes relative abundance being observed.					
35682591	5	39	theme	increased	777:785	arg1	Firmicutes					787:796	increased Firmicutes	777:796	increased Firmicutes	777:796	Moreover, HACS also greatly impacted the gut microbiota with increased Firmicutes and decreased Bacteroidetes relative abundance being observed.					
35682591	5	40	theme	relative	826:833	arg1	abundance					835:843	Bacteroidetes relative abundance	812:843	Bacteroidetes relative abundance being observed	812:858	Moreover, HACS also greatly impacted the gut microbiota with increased Firmicutes and decreased Bacteroidetes relative abundance being observed.					
35682591	3	41	theme	starch	417:422	arg1	influence					386:394	the influence	382:394	the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids	382:487	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	0	42	theme	Gut	35:37	arg1	Microbiota					39:48	Gut Microbiota	35:48	Gut Microbiota	35:48	High-Amylose Corn Starch Regulated Gut Microbiota and Serum Bile Acids in High-Fat Diet-Induced Obese Mice.					
35682591	6	43	with	mice	1002:1005	arg1	intervention					1021:1032	the HACS intervention	1012:1032	the HACS intervention	1012:1032	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	7	44	theme	regulatory	1235:1244	arg1	effects					1246:1252	its regulatory effects	1231:1252	its regulatory effects on gut microbiota and circulating bile acids	1231:1297	In summary, our results suggested that the underlying mechanisms of anti-obesity activity of HACS may involve its regulatory effects on gut microbiota and circulating bile acids.					
35682591	0	45	theme	Serum	54:58	arg1	Acids					65:69	Serum Bile Acids	54:69	Serum Bile Acids	54:69	High-Amylose Corn Starch Regulated Gut Microbiota and Serum Bile Acids in High-Fat Diet-Induced Obese Mice.					
35682591	1	46	from	effects	218:224	arg1	microbiota					233:242	gut microbiota	229:242	gut microbiota	229:242	High-amylose corn starch is well known for its anti-obesity activity, which is mainly based on the regulatory effects on gut microbiota.					
35682591	3	47	theme	high-amylose	399:410	arg1	HACS					425:428	HACS	425:428	HACS	425:428	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	3	47	theme	high-amylose	399:410	arg1	starch					417:422	high-amylose corn starch	399:422	high-amylose corn starch (HACS)	399:429	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	3	48	theme	fat	526:528	arg1	HFD					536:538	HFD	536:538	HFD	536:538	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	3	48	theme	fat	526:528	arg1	diet					530:533	a high fat diet	519:533	a high fat diet (HFD)	519:539	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	3	49	theme	microbiota	445:454	arg1	composition					456:466	intestinal microbiota composition	434:466	intestinal microbiota composition	434:466	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	7	50	theme	circulating	1276:1286	arg1	acids					1293:1297	circulating bile acids	1276:1297	circulating bile acids	1276:1297	In summary, our results suggested that the underlying mechanisms of anti-obesity activity of HACS may involve its regulatory effects on gut microbiota and circulating bile acids.					
35682591	1	51	theme	corn	121:124	arg1	starch					126:131	High-amylose corn starch	108:131	High-amylose corn starch	108:131	High-amylose corn starch is well known for its anti-obesity activity, which is mainly based on the regulatory effects on gut microbiota.					
35682591	6	52	theme	ND-fed	886:891	arg1	mice					893:896	ND-fed mice	886:896	ND-fed mice	886:896	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	2	53	theme	metabolic	303:311	arg1	health					313:318	metabolic health	303:318	metabolic health	303:318	Recently, the gut microbiota has been reported to improve metabolic health by altering circulating bile acids.					
35682591	6	54	theme	bile	1089:1092	arg1	composition					1099:1109	bile acid composition	1089:1109	bile acid composition in blood	1089:1118	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	6	55	theme	acids	970:974	arg1	profiles					976:983	serum bile acids profiles	959:983	serum bile acids profiles	959:983	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	6	56	with	mice	903:906	arg1	feeding					917:923	HFD feeding	913:923	HFD feeding	913:923	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	3	57	from	influence	386:394	arg1	composition					456:466	intestinal microbiota composition	434:466	intestinal microbiota composition	434:466	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	3	57	from	influence	386:394	arg1	acids					483:487	serum bile acids	472:487	serum bile acids	472:487	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	3	58	theme	intestinal	434:443	arg1	composition					456:466	intestinal microbiota composition	434:466	intestinal microbiota composition	434:466	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	1	59	theme	regulatory	207:216	arg1	effects					218:224	the regulatory effects	203:224	the regulatory effects on gut microbiota	203:242	High-amylose corn starch is well known for its anti-obesity activity, which is mainly based on the regulatory effects on gut microbiota.					
35682591	7	60	theme	underlying	1164:1173	arg1	mechanisms					1175:1184	the underlying mechanisms	1160:1184	the underlying mechanisms of anti-obesity activity of HACS	1160:1217	In summary, our results suggested that the underlying mechanisms of anti-obesity activity of HACS may involve its regulatory effects on gut microbiota and circulating bile acids.					
35682591	7	61	theme	bile	1288:1291	arg1	acids					1293:1297	circulating bile acids	1276:1297	circulating bile acids	1276:1297	In summary, our results suggested that the underlying mechanisms of anti-obesity activity of HACS may involve its regulatory effects on gut microbiota and circulating bile acids.					
35682591	3	62	theme	bile	478:481	arg1	acids					483:487	serum bile acids	472:487	serum bile acids	472:487	Therefore, in this study, the influence of high-amylose corn starch (HACS) on intestinal microbiota composition and serum bile acids was explored in mice fed with a high fat diet (HFD).					
35682591	4	63	theme	HFD-induced	590:600	arg1	gain					614:617	HFD-induced body weight gain	590:617	HFD-induced body weight gain	590:617	The results demonstrated HACS treatment reduced HFD-induced body weight gain, hepatic lipid accumulation, and adipocyte hypertrophy as well as improved blood lipid profiles.					
35682591	6	64	theme	serum	959:963	arg1	acids					970:974	serum bile acids	959:974	serum bile acids profiles	959:983	Furthermore, compared to ND-fed mice, the mice with HFD feeding exhibited more obvious changes in serum bile acids profiles than the HFD-fed mice with the HACS intervention, showing HACS might restore HFD-induced alterations to bile acid composition in blood.					
35682591	0	65	theme	Bile	60:63	arg1	Acids					65:69	Serum Bile Acids	54:69	Serum Bile Acids	54:69	High-Amylose Corn Starch Regulated Gut Microbiota and Serum Bile Acids in High-Fat Diet-Induced Obese Mice.					
35682591	2	66	theme	bile	344:347	arg1	acids					349:353	circulating bile acids	332:353	circulating bile acids	332:353	Recently, the gut microbiota has been reported to improve metabolic health by altering circulating bile acids.					
37172419	8	0	dep	suggests	1489:1496	arg1	Taken					1469:1473	Taken	1469:1473	Taken together	1469:1482	Taken together this suggests that sialic acids are important for lubrication and may exert this through the sacrificial layer mechanism.					
37172419	4	1	theme	ionic	680:684	arg1	concentration					686:698	the ionic concentration	676:698	the ionic concentration in mucin films	676:713	The ionic concentration can vary significantly in different mucus systems and here we show that increasing the ionic concentration in mucin films leads to an increase in lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic.					
37172419	2	2	theme	hydrated	325:332	arg1	biogel					334:339	a hydrated biogel	323:339	a hydrated biogel	323:339	Mucins, the main macromolecule, are heavily glycosylated proteins that polymerise and retain water molecules, resulting in a hydrated biogel.					
37172419	0	3	from	influence	4:12	arg1	role					68:71	the role	64:71	the role of sialic acids	64:87	The influence of ions on the lubricative abilities of mucin and the role of sialic acids.					
37172419	0	3	from	influence	4:12	arg1	abilities					41:49	the lubricative abilities	25:49	the lubricative abilities of mucin	25:58	The influence of ions on the lubricative abilities of mucin and the role of sialic acids.					
37172419	8	4	theme	sialic	1503:1508	arg1	acids					1510:1514	sialic acids	1503:1514	sialic acids	1503:1514	Taken together this suggests that sialic acids are important for lubrication and may exert this through the sacrificial layer mechanism.					
37172419	6	5	theme	mucin	1267:1271	arg1	films					1273:1277	mucin films	1267:1277	mucin films with increasing ionic concentrations	1267:1314	Furthermore, we determined that the removal of negatively charged sialic acid moieties by sialidase digestion resulted in reduced adsorption to hydrophilic surfaces but did not affect the swelling of mucin films with increasing ionic concentrations.					
37172419	6	6	theme	increasing	1284:1293	arg1	concentrations					1301:1314	increasing ionic concentrations	1284:1314	increasing ionic concentrations	1284:1314	Furthermore, we determined that the removal of negatively charged sialic acid moieties by sialidase digestion resulted in reduced adsorption to hydrophilic surfaces but did not affect the swelling of mucin films with increasing ionic concentrations.					
37172419	6	7	theme	films	1273:1277	arg1	swelling					1255:1262	the swelling	1251:1262	the swelling of mucin films with increasing ionic concentrations	1251:1314	Furthermore, we determined that the removal of negatively charged sialic acid moieties by sialidase digestion resulted in reduced adsorption to hydrophilic surfaces but did not affect the swelling of mucin films with increasing ionic concentrations.					
37172419	3	8	from	glycans	493:499	arg1	moieties					510:517	mucin moieties	504:517	mucin moieties	504:517	It is assumed that positively charged ions can influence mucin film structure by screening the electrostatic repulsions between the negatively charged glycans on mucin moieties and draw in water molecules via hydration shells.					
37172419	7	9	theme	ionic	1447:1451	arg1	concentrations					1453:1466	ionic concentrations	1447:1466	ionic concentrations	1447:1466	Moreover, the coefficient of friction was increased with sialic acid removal, but lubrication was still increased with increasing ionic concentrations.					
37172419	4	10	theme	ionic	573:577	arg1	concentration					579:591	The ionic concentration	569:591	The ionic concentration	569:591	The ionic concentration can vary significantly in different mucus systems and here we show that increasing the ionic concentration in mucin films leads to an increase in lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic.					
37172419	5	11	theme	ionic	927:931	arg1	concentration					933:945	increased ionic concentration	917:945	increased ionic concentration	917:945	Mucins were found to bind sodium ions in a concentration-dependent manner and increased ionic concentration appears to cause mucin films to swell when assessed by Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis.					
37172419	6	12	theme	moieties	1145:1152	arg1	removal					1103:1109	the removal	1099:1109	the removal of negatively charged sialic acid moieties by sialidase digestion	1099:1175	Furthermore, we determined that the removal of negatively charged sialic acid moieties by sialidase digestion resulted in reduced adsorption to hydrophilic surfaces but did not affect the swelling of mucin films with increasing ionic concentrations.					
37172419	5	13	theme	mucin	964:968	arg1	films					970:974	mucin films	964:974	mucin films to swell	964:983	Mucins were found to bind sodium ions in a concentration-dependent manner and increased ionic concentration appears to cause mucin films to swell when assessed by Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis.					
37172419	3	14	theme	charged	485:491	arg1	glycans					493:499	the negatively charged glycans	470:499	the negatively charged glycans on mucin moieties	470:517	It is assumed that positively charged ions can influence mucin film structure by screening the electrostatic repulsions between the negatively charged glycans on mucin moieties and draw in water molecules via hydration shells.					
37172419	4	15	theme	mucin	703:707	arg1	films					709:713	mucin films	703:713	mucin films	703:713	The ionic concentration can vary significantly in different mucus systems and here we show that increasing the ionic concentration in mucin films leads to an increase in lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic.					
37172419	0	16	theme	acids	83:87	arg1	role					68:71	the role	64:71	the role of sialic acids	64:87	The influence of ions on the lubricative abilities of mucin and the role of sialic acids.					
37172419	0	16	theme	acids	83:87	arg1	abilities					41:49	the lubricative abilities	25:49	the lubricative abilities of mucin	25:58	The influence of ions on the lubricative abilities of mucin and the role of sialic acids.					
37172419	6	17	theme	charged	1125:1131	arg1	moieties					1145:1152	negatively charged sialic acid moieties	1114:1152	negatively charged sialic acid moieties	1114:1152	Furthermore, we determined that the removal of negatively charged sialic acid moieties by sialidase digestion resulted in reduced adsorption to hydrophilic surfaces but did not affect the swelling of mucin films with increasing ionic concentrations.					
37172419	7	18	theme	sialic	1374:1379	arg1	acid					1381:1384	sialic acid	1374:1384	sialic acid removal	1374:1392	Moreover, the coefficient of friction was increased with sialic acid removal, but lubrication was still increased with increasing ionic concentrations.					
37172419	2	19	theme	glycosylated	244:255	arg1	proteins					257:264	heavily glycosylated proteins	236:264	heavily glycosylated proteins that polymerise and retain water molecules	236:307	Mucins, the main macromolecule, are heavily glycosylated proteins that polymerise and retain water molecules, resulting in a hydrated biogel.					
37172419	2	19	theme	glycosylated	244:255	arg1	Mucins					200:205	Mucins	200:205	Mucins	200:205	Mucins, the main macromolecule, are heavily glycosylated proteins that polymerise and retain water molecules, resulting in a hydrated biogel.					
37172419	7	20	theme	friction	1346:1353	arg1	coefficient					1331:1341	the coefficient	1327:1341	the coefficient of friction	1327:1353	Moreover, the coefficient of friction was increased with sialic acid removal, but lubrication was still increased with increasing ionic concentrations.					
37172419	3	21	theme	film	405:408	arg1	structure					410:418	mucin film structure	399:418	mucin film structure	399:418	It is assumed that positively charged ions can influence mucin film structure by screening the electrostatic repulsions between the negatively charged glycans on mucin moieties and draw in water molecules via hydration shells.					
37172419	0	22	theme	sialic	76:81	arg1	acids					83:87	sialic acids	76:87	sialic acids	76:87	The influence of ions on the lubricative abilities of mucin and the role of sialic acids.					
37172419	4	23	from	increase	727:734	arg1	lubrication					739:749	lubrication	739:749	lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic	739:836	The ionic concentration can vary significantly in different mucus systems and here we show that increasing the ionic concentration in mucin films leads to an increase in lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic.					
37172419	6	24	theme	sialidase	1157:1165	arg1	digestion					1167:1175	sialidase digestion	1157:1175	sialidase digestion	1157:1175	Furthermore, we determined that the removal of negatively charged sialic acid moieties by sialidase digestion resulted in reduced adsorption to hydrophilic surfaces but did not affect the swelling of mucin films with increasing ionic concentrations.					
37172419	2	25	theme	water	293:297	arg1	molecules					299:307	water molecules	293:307	water molecules	293:307	Mucins, the main macromolecule, are heavily glycosylated proteins that polymerise and retain water molecules, resulting in a hydrated biogel.					
37172419	3	26	theme	hydration	551:559	arg1	shells					561:566	hydration shells	551:566	hydration shells	551:566	It is assumed that positively charged ions can influence mucin film structure by screening the electrostatic repulsions between the negatively charged glycans on mucin moieties and draw in water molecules via hydration shells.					
37172419	3	27	theme	mucin	399:403	arg1	structure					410:418	mucin film structure	399:418	mucin film structure	399:418	It is assumed that positively charged ions can influence mucin film structure by screening the electrostatic repulsions between the negatively charged glycans on mucin moieties and draw in water molecules via hydration shells.					
37172419	0	28	theme	ions	17:20	arg1	influence					4:12	The influence	0:12	The influence of ions on the lubricative abilities of mucin and the role of sialic acids	0:87	The influence of ions on the lubricative abilities of mucin and the role of sialic acids.					
37172419	4	29	theme	sliding	796:802	arg1	contact					804:810	sliding contact	796:810	sliding contact in a compliant oral mimic	796:836	The ionic concentration can vary significantly in different mucus systems and here we show that increasing the ionic concentration in mucin films leads to an increase in lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic.					
37172419	5	30	theme	increased	917:925	arg1	concentration					933:945	increased ionic concentration	917:945	increased ionic concentration	917:945	Mucins were found to bind sodium ions in a concentration-dependent manner and increased ionic concentration appears to cause mucin films to swell when assessed by Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis.					
37172419	3	31	theme	water	531:535	arg1	molecules					537:545	water molecules	531:545	water molecules	531:545	It is assumed that positively charged ions can influence mucin film structure by screening the electrostatic repulsions between the negatively charged glycans on mucin moieties and draw in water molecules via hydration shells.					
37172419	4	32	theme	different	619:627	arg1	systems					635:641	different mucus systems	619:641	different mucus systems	619:641	The ionic concentration can vary significantly in different mucus systems and here we show that increasing the ionic concentration in mucin films leads to an increase in lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic.					
37172419	4	33	dep	oral	827:830	arg1	mimic					832:836	mimic	832:836	mimic	832:836	The ionic concentration can vary significantly in different mucus systems and here we show that increasing the ionic concentration in mucin films leads to an increase in lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic.					
37172419	8	34	theme	sacrificial	1577:1587	arg1	mechanism					1595:1603	the sacrificial layer mechanism	1573:1603	the sacrificial layer mechanism	1573:1603	Taken together this suggests that sialic acids are important for lubrication and may exert this through the sacrificial layer mechanism.					
37172419	6	35	theme	sialic	1133:1138	arg1	moieties					1145:1152	negatively charged sialic acid moieties	1114:1152	negatively charged sialic acid moieties	1114:1152	Furthermore, we determined that the removal of negatively charged sialic acid moieties by sialidase digestion resulted in reduced adsorption to hydrophilic surfaces but did not affect the swelling of mucin films with increasing ionic concentrations.					
37172419	3	36	theme	electrostatic	437:449	arg1	repulsions					451:460	the electrostatic repulsions	433:460	the electrostatic repulsions between the negatively charged glycans on mucin moieties	433:517	It is assumed that positively charged ions can influence mucin film structure by screening the electrostatic repulsions between the negatively charged glycans on mucin moieties and draw in water molecules via hydration shells.					
37172419	5	37	theme	Quartz	1002:1007	arg1	analysis					1057:1064	Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis	1002:1064	Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis	1002:1064	Mucins were found to bind sodium ions in a concentration-dependent manner and increased ionic concentration appears to cause mucin films to swell when assessed by Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis.					
37172419	1	38	theme	mixed	186:190	arg1	regime					192:197	mixed regime	186:197	mixed regime	186:197	Mucus reduces friction between epithelial surfaces by providing lubrication in the boundary and mixed regime.					
37172419	5	39	theme	Crystal	1009:1015	arg1	analysis					1057:1064	Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis	1002:1064	Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis	1002:1064	Mucins were found to bind sodium ions in a concentration-dependent manner and increased ionic concentration appears to cause mucin films to swell when assessed by Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis.					
37172419	7	40	theme	acid	1381:1384	arg1	removal					1386:1392	sialic acid removal	1374:1392	sialic acid removal	1374:1392	Moreover, the coefficient of friction was increased with sialic acid removal, but lubrication was still increased with increasing ionic concentrations.					
37172419	2	41	gly	glycosylated	244:255	arg1	proteins					257:264	heavily glycosylated proteins	236:264	heavily glycosylated proteins that polymerise and retain water molecules	236:307	Mucins, the main macromolecule, are heavily glycosylated proteins that polymerise and retain water molecules, resulting in a hydrated biogel.					
37172419	2	41	gly	glycosylated	244:255	arg1	Mucins					200:205	Mucins	200:205	Mucins	200:205	Mucins, the main macromolecule, are heavily glycosylated proteins that polymerise and retain water molecules, resulting in a hydrated biogel.					
37172419	9	42	dep	acids	1693:1697	arg1	partly					1709:1714	partly	1709:1714	partly	1709:1714	Ionic concentration appears to influence mucin films and their lubrication, and sialic acids, at least partly, may be important for ion binding.					
37172419	5	43	theme	sodium	865:870	arg1	ions					872:875	sodium ions	865:875	sodium ions	865:875	Mucins were found to bind sodium ions in a concentration-dependent manner and increased ionic concentration appears to cause mucin films to swell when assessed by Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis.					
37172419	5	44	theme	hiMicrobalance	1017:1030	arg1	analysis					1057:1064	Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis	1002:1064	Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis	1002:1064	Mucins were found to bind sodium ions in a concentration-dependent manner and increased ionic concentration appears to cause mucin films to swell when assessed by Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis.					
37172419	5	45	theme	concentration-dependent	882:904	arg1	manner					906:911	a concentration-dependent manner	880:911	a concentration-dependent manner	880:911	Mucins were found to bind sodium ions in a concentration-dependent manner and increased ionic concentration appears to cause mucin films to swell when assessed by Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis.					
37172419	0	46	theme	lubricative	29:39	arg1	abilities					41:49	the lubricative abilities	25:49	the lubricative abilities of mucin	25:58	The influence of ions on the lubricative abilities of mucin and the role of sialic acids.					
37172419	5	47	theme	Dissipation	1037:1047	arg1	analysis					1057:1064	Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis	1002:1064	Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis	1002:1064	Mucins were found to bind sodium ions in a concentration-dependent manner and increased ionic concentration appears to cause mucin films to swell when assessed by Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis.					
37172419	5	48	theme	with	1032:1035	arg1	analysis					1057:1064	Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis	1002:1064	Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis	1002:1064	Mucins were found to bind sodium ions in a concentration-dependent manner and increased ionic concentration appears to cause mucin films to swell when assessed by Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis.					
37172419	9	49	theme	sialic	1686:1691	arg1	acids					1693:1697	sialic acids	1686:1697	sialic acids	1686:1697	Ionic concentration appears to influence mucin films and their lubrication, and sialic acids, at least partly, may be important for ion binding.					
37172419	6	50	with	films	1273:1277	arg1	concentrations					1301:1314	increasing ionic concentrations	1284:1314	increasing ionic concentrations	1284:1314	Furthermore, we determined that the removal of negatively charged sialic acid moieties by sialidase digestion resulted in reduced adsorption to hydrophilic surfaces but did not affect the swelling of mucin films with increasing ionic concentrations.					
37172419	6	51	theme	reduced	1189:1195	arg1	adsorption					1197:1206	reduced adsorption	1189:1206	reduced adsorption to hydrophilic surfaces	1189:1230	Furthermore, we determined that the removal of negatively charged sialic acid moieties by sialidase digestion resulted in reduced adsorption to hydrophilic surfaces but did not affect the swelling of mucin films with increasing ionic concentrations.					
37172419	9	52	theme	ion	1738:1740	arg1	binding					1742:1748	ion binding	1738:1748	ion binding	1738:1748	Ionic concentration appears to influence mucin films and their lubrication, and sialic acids, at least partly, may be important for ion binding.					
37172419	9	53	theme	mucin	1647:1651	arg1	films					1653:1657	mucin films	1647:1657	mucin films	1647:1657	Ionic concentration appears to influence mucin films and their lubrication, and sialic acids, at least partly, may be important for ion binding.					
37172419	6	54	theme	hydrophilic	1211:1221	arg1	surfaces					1223:1230	hydrophilic surfaces	1211:1230	hydrophilic surfaces	1211:1230	Furthermore, we determined that the removal of negatively charged sialic acid moieties by sialidase digestion resulted in reduced adsorption to hydrophilic surfaces but did not affect the swelling of mucin films with increasing ionic concentrations.					
37172419	4	55	from	contact	804:810	arg1	oral					827:830	oral	827:830	oral	827:830	The ionic concentration can vary significantly in different mucus systems and here we show that increasing the ionic concentration in mucin films leads to an increase in lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic.					
37172419	4	55	from	contact	804:810	arg1	surfaces					784:791	two polydimethylsiloxane surfaces	759:791	two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic	759:836	The ionic concentration can vary significantly in different mucus systems and here we show that increasing the ionic concentration in mucin films leads to an increase in lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic.					
37172419	9	56	theme	Ionic	1606:1610	arg1	concentration					1612:1624	Ionic concentration	1606:1624	Ionic concentration	1606:1624	Ionic concentration appears to influence mucin films and their lubrication, and sialic acids, at least partly, may be important for ion binding.					
37172419	6	57	theme	acid	1140:1143	arg1	moieties					1145:1152	negatively charged sialic acid moieties	1114:1152	negatively charged sialic acid moieties	1114:1152	Furthermore, we determined that the removal of negatively charged sialic acid moieties by sialidase digestion resulted in reduced adsorption to hydrophilic surfaces but did not affect the swelling of mucin films with increasing ionic concentrations.					
37172419	6	58	theme	ionic	1295:1299	arg1	concentrations					1301:1314	increasing ionic concentrations	1284:1314	increasing ionic concentrations	1284:1314	Furthermore, we determined that the removal of negatively charged sialic acid moieties by sialidase digestion resulted in reduced adsorption to hydrophilic surfaces but did not affect the swelling of mucin films with increasing ionic concentrations.					
37172419	5	59	theme	QCM-D	1050:1054	arg1	analysis					1057:1064	Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis	1002:1064	Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis	1002:1064	Mucins were found to bind sodium ions in a concentration-dependent manner and increased ionic concentration appears to cause mucin films to swell when assessed by Quartz Crystal hiMicrobalance with Dissipation (QCM-D) analysis.					
37172419	4	60	theme	polydimethylsiloxane	763:782	arg1	surfaces					784:791	two polydimethylsiloxane surfaces	759:791	two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic	759:836	The ionic concentration can vary significantly in different mucus systems and here we show that increasing the ionic concentration in mucin films leads to an increase in lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic.					
37172419	1	61	theme	epithelial	121:130	arg1	surfaces					132:139	epithelial surfaces	121:139	epithelial surfaces	121:139	Mucus reduces friction between epithelial surfaces by providing lubrication in the boundary and mixed regime.					
37172419	0	62	theme	mucin	54:58	arg1	role					68:71	the role	64:71	the role of sialic acids	64:87	The influence of ions on the lubricative abilities of mucin and the role of sialic acids.					
37172419	0	62	theme	mucin	54:58	arg1	abilities					41:49	the lubricative abilities	25:49	the lubricative abilities of mucin	25:58	The influence of ions on the lubricative abilities of mucin and the role of sialic acids.					
37172419	4	63	theme	mucus	629:633	arg1	systems					635:641	different mucus systems	619:641	different mucus systems	619:641	The ionic concentration can vary significantly in different mucus systems and here we show that increasing the ionic concentration in mucin films leads to an increase in lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic.					
37172419	1	64	dep	boundary	173:180	arg1	the					169:171	the	169:171	the	169:171	Mucus reduces friction between epithelial surfaces by providing lubrication in the boundary and mixed regime.					
37172419	3	65	theme	charged	372:378	arg1	ions					380:383	positively charged ions	361:383	positively charged ions	361:383	It is assumed that positively charged ions can influence mucin film structure by screening the electrostatic repulsions between the negatively charged glycans on mucin moieties and draw in water molecules via hydration shells.					
37172419	4	66	from	concentration	686:698	arg1	films					709:713	mucin films	703:713	mucin films	703:713	The ionic concentration can vary significantly in different mucus systems and here we show that increasing the ionic concentration in mucin films leads to an increase in lubrication between two polydimethylsiloxane surfaces at sliding contact in a compliant oral mimic.					
37172419	2	67	theme	main	212:215	arg1	macromolecule					217:229	the main macromolecule	208:229	the main macromolecule	208:229	Mucins, the main macromolecule, are heavily glycosylated proteins that polymerise and retain water molecules, resulting in a hydrated biogel.					
37172419	2	67	theme	main	212:215	arg1	Mucins					200:205	Mucins	200:205	Mucins	200:205	Mucins, the main macromolecule, are heavily glycosylated proteins that polymerise and retain water molecules, resulting in a hydrated biogel.					
37172419	3	68	theme	mucin	504:508	arg1	moieties					510:517	mucin moieties	504:517	mucin moieties	504:517	It is assumed that positively charged ions can influence mucin film structure by screening the electrostatic repulsions between the negatively charged glycans on mucin moieties and draw in water molecules via hydration shells.					
37172419	8	69	theme	layer	1589:1593	arg1	mechanism					1595:1603	the sacrificial layer mechanism	1573:1603	the sacrificial layer mechanism	1573:1603	Taken together this suggests that sialic acids are important for lubrication and may exert this through the sacrificial layer mechanism.					
36241290	4	0	theme	reinforcing	645:655	arg1	agents					657:662	the reinforcing agents	641:662	the reinforcing agents	641:662	XRD results showed that there were physical interactions between the reinforcing agents and PVA, which improved the thermal stability of the film.					
36241290	1	1	theme	modified	144:151	arg1	MCNC					177:180	MCNC	177:180	MCNC	177:180	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	1	theme	modified	144:151	arg1	aerogel					194:200	silica aerogel	187:200	silica aerogel (SA)	187:205	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	1	theme	modified	144:151	arg1	nanocrystals					163:174	citric acid modified cellulose nanocrystals	132:174	citric acid modified cellulose nanocrystals (MCNC)	132:181	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	1	theme	modified	144:151	arg1	agents					232:237	reinforcing agents	220:237	reinforcing agents	220:237	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	6	2	theme	carbon	828:833	arg1	dioxide					835:841	carbon dioxide	828:841	The carbon dioxide permeability	824:854	The carbon dioxide permeability decreased by 66.01 % and the oxygen permeability decreased by 69.46 % (23 °C, 50 % RH) and 40.14 % (38 °C, 90 % RH).					
36241290	0	3	theme	cellulose	73:81	arg1	nanocrystals					83:94	citric acid modified cellulose nanocrystals	52:94	citric acid modified cellulose nanocrystals	52:94	Properties of polyvinyl alcohol films reinforced by citric acid modified cellulose nanocrystals and silica aerogels.					
36241290	1	4	theme	cellulose	153:161	arg1	MCNC					177:180	MCNC	177:180	MCNC	177:180	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	4	theme	cellulose	153:161	arg1	aerogel					194:200	silica aerogel	187:200	silica aerogel (SA)	187:205	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	4	theme	cellulose	153:161	arg1	nanocrystals					163:174	citric acid modified cellulose nanocrystals	132:174	citric acid modified cellulose nanocrystals (MCNC)	132:181	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	4	theme	cellulose	153:161	arg1	agents					232:237	reinforcing agents	220:237	reinforcing agents	220:237	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	5	theme	reinforcing	220:230	arg1	aerogel					194:200	silica aerogel	187:200	silica aerogel (SA)	187:205	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	5	theme	reinforcing	220:230	arg1	nanocrystals					163:174	citric acid modified cellulose nanocrystals	132:174	citric acid modified cellulose nanocrystals (MCNC)	132:181	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	5	theme	reinforcing	220:230	arg1	agents					232:237	reinforcing agents	220:237	reinforcing agents	220:237	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	0	6	theme	silica	100:105	arg1	aerogels					107:114	silica aerogels	100:114	silica aerogels	100:114	Properties of polyvinyl alcohol films reinforced by citric acid modified cellulose nanocrystals and silica aerogels.					
36241290	6	7	theme	dioxide	835:841	arg1	permeability					843:854	The carbon dioxide permeability	824:854	The carbon dioxide permeability	824:854	The carbon dioxide permeability decreased by 66.01 % and the oxygen permeability decreased by 69.46 % (23 °C, 50 % RH) and 40.14 % (38 °C, 90 % RH).					
36241290	4	8	theme	film	717:720	arg1	stability					700:708	the thermal stability	688:708	the thermal stability of the film	688:720	XRD results showed that there were physical interactions between the reinforcing agents and PVA, which improved the thermal stability of the film.					
36241290	1	9	theme	composite	312:320	arg1	films					322:326	composite films	312:326	composite films with excellent mechanical and barrier properties	312:375	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	7	10	theme	tensile	977:983	arg1	strength					985:992	The tensile strength	973:992	The tensile strength	973:992	The tensile strength increased to 43.79 MPa.					
36241290	6	11	dep	%	953:953	arg1	38 °C					956:960	38 °C	956:960	38 °C	956:960	The carbon dioxide permeability decreased by 66.01 % and the oxygen permeability decreased by 69.46 % (23 °C, 50 % RH) and 40.14 % (38 °C, 90 % RH).					
36241290	6	11	dep	%	953:953	arg1	%					966:966	90 % RH	963:969	90 % RH	963:969	The carbon dioxide permeability decreased by 66.01 % and the oxygen permeability decreased by 69.46 % (23 °C, 50 % RH) and 40.14 % (38 °C, 90 % RH).					
36241290	4	12	theme	physical	611:618	arg1	interactions					620:631	physical interactions	611:631	physical interactions between the reinforcing agents and PVA, which improved the thermal stability of the film	611:720	XRD results showed that there were physical interactions between the reinforcing agents and PVA, which improved the thermal stability of the film.					
36241290	8	13	theme	composite	1033:1041	arg1	film					1043:1046	the composite film	1029:1046	the composite film	1029:1046	Therefore, the composite film can be widely used in the packaging of food, medicine, etc.					
36241290	1	14	from	alcohol	262:268	arg1	proportions					289:299	different proportions	279:299	different proportions	279:299	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	8	15	theme	food	1087:1090	arg1	packaging					1074:1082	the packaging	1070:1082	the packaging of food, medicine, etc	1070:1105	Therefore, the composite film can be widely used in the packaging of food, medicine, etc.					
36241290	2	16	with	modification	384:395	arg1	acid					409:412	citric acid	402:412	citric acid	402:412	After modification with citric acid, MCNC successfully contained ester bonds.					
36241290	2	17	contain	contained	433:441	arg2	bonds					449:453	ester bonds	443:453	ester bonds	443:453	After modification with citric acid, MCNC successfully contained ester bonds.					
36241290	2	17	contain	contained	433:441	arg1	MCNC					415:418	MCNC	415:418	MCNC	415:418	After modification with citric acid, MCNC successfully contained ester bonds.					
36241290	4	18	theme	thermal	692:698	arg1	stability					700:708	the thermal stability	688:708	the thermal stability of the film	688:720	XRD results showed that there were physical interactions between the reinforcing agents and PVA, which improved the thermal stability of the film.					
36241290	5	19	theme	1.5 	814:817	arg1	%					818:818	%	818:818	%	818:818	Meanwhile, the composite film obtained good barrier properties after adding 0.5 % MCNC and 1.5 % SA.					
36241290	5	20	theme	good	762:765	arg1	properties					775:784	good barrier properties	762:784	good barrier properties	762:784	Meanwhile, the composite film obtained good barrier properties after adding 0.5 % MCNC and 1.5 % SA.					
36241290	3	21	theme	PVA	533:535	arg1	matrix					537:542	the PVA matrix	529:542	the PVA matrix	529:542	SEM and FTIR results showed that MCNC and SA were uniformly dispersed in the PVA matrix and formed a compact structure.					
36241290	5	22	theme	%	818:818	arg1	SA					820:821	1.5 % SA	814:821	1.5 % SA	814:821	Meanwhile, the composite film obtained good barrier properties after adding 0.5 % MCNC and 1.5 % SA.					
36241290	0	23	theme	alcohol	24:30	arg1	films					32:36	polyvinyl alcohol films	14:36	polyvinyl alcohol films	14:36	Properties of polyvinyl alcohol films reinforced by citric acid modified cellulose nanocrystals and silica aerogels.					
36241290	5	24	theme	composite	738:746	arg1	film					748:751	the composite film	734:751	the composite film	734:751	Meanwhile, the composite film obtained good barrier properties after adding 0.5 % MCNC and 1.5 % SA.					
36241290	5	24	theme	composite	738:746	arg1	Meanwhile					723:731	Meanwhile	723:731	Meanwhile	723:731	Meanwhile, the composite film obtained good barrier properties after adding 0.5 % MCNC and 1.5 % SA.					
36241290	0	25	theme	polyvinyl	14:22	arg1	alcohol					24:30	polyvinyl alcohol	14:30	polyvinyl alcohol films	14:36	Properties of polyvinyl alcohol films reinforced by citric acid modified cellulose nanocrystals and silica aerogels.					
36241290	1	26	theme	polyvinyl	252:260	arg1	PVA					271:273	PVA	271:273	PVA	271:273	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	26	theme	polyvinyl	252:260	arg1	alcohol					262:268	polyvinyl alcohol	252:268	polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties	252:375	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	8	27	used	used	1062:1065	arg2	film					1043:1046	the composite film	1029:1046	the composite film	1029:1046	Therefore, the composite film can be widely used in the packaging of food, medicine, etc.					
36241290	1	28	theme	silica	187:192	arg1	agents					232:237	reinforcing agents	220:237	reinforcing agents	220:237	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	28	theme	silica	187:192	arg1	SA					203:204	SA	203:204	SA	203:204	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	28	theme	silica	187:192	arg1	aerogel					194:200	silica aerogel	187:200	silica aerogel (SA)	187:205	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	28	theme	silica	187:192	arg1	nanocrystals					163:174	citric acid modified cellulose nanocrystals	132:174	citric acid modified cellulose nanocrystals (MCNC)	132:181	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	6	29	theme	oxygen	885:890	arg1	permeability					892:903	the oxygen permeability	881:903	the oxygen permeability	881:903	The carbon dioxide permeability decreased by 66.01 % and the oxygen permeability decreased by 69.46 % (23 °C, 50 % RH) and 40.14 % (38 °C, 90 % RH).					
36241290	5	30	theme	0.5 	799:802	arg1	%					803:803	%	803:803	%	803:803	Meanwhile, the composite film obtained good barrier properties after adding 0.5 % MCNC and 1.5 % SA.					
36241290	0	31	theme	films	32:36	arg1	Properties					0:9	Properties	0:9	Properties of polyvinyl alcohol films	0:36	Properties of polyvinyl alcohol films reinforced by citric acid modified cellulose nanocrystals and silica aerogels.					
36241290	2	32	theme	ester	443:447	arg1	bonds					449:453	ester bonds	443:453	ester bonds	443:453	After modification with citric acid, MCNC successfully contained ester bonds.					
36241290	1	33	theme	barrier	358:364	arg1	properties					366:375	excellent mechanical and barrier properties	333:375	properties	366:375	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	5	34	theme	barrier	767:773	arg1	properties					775:784	good barrier properties	762:784	good barrier properties	762:784	Meanwhile, the composite film obtained good barrier properties after adding 0.5 % MCNC and 1.5 % SA.					
36241290	5	35	theme	%	803:803	arg1	MCNC					805:808	0.5 % MCNC	799:808	0.5 % MCNC	799:808	Meanwhile, the composite film obtained good barrier properties after adding 0.5 % MCNC and 1.5 % SA.					
36241290	0	36	theme	citric	52:57	arg1	nanocrystals					83:94	citric acid modified cellulose nanocrystals	52:94	citric acid modified cellulose nanocrystals	52:94	Properties of polyvinyl alcohol films reinforced by citric acid modified cellulose nanocrystals and silica aerogels.					
36241290	1	37	used	used	212:215	arg2	nanocrystals					163:174	citric acid modified cellulose nanocrystals	132:174	citric acid modified cellulose nanocrystals (MCNC)	132:181	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	37	used	used	212:215	arg2	aerogel					194:200	silica aerogel	187:200	silica aerogel (SA)	187:205	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	37	used	used	212:215	arg2	agents					232:237	reinforcing agents	220:237	reinforcing agents	220:237	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	37	used	used	212:215	arg2	SA					203:204	SA	203:204	SA	203:204	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	37	used	used	212:215	arg2	MCNC					177:180	MCNC	177:180	MCNC	177:180	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	3	38	theme	FTIR	464:467	arg1	results					469:475	FTIR results	464:475	FTIR results	464:475	SEM and FTIR results showed that MCNC and SA were uniformly dispersed in the PVA matrix and formed a compact structure.					
36241290	0	39	theme	modified	64:71	arg1	nanocrystals					83:94	citric acid modified cellulose nanocrystals	52:94	citric acid modified cellulose nanocrystals	52:94	Properties of polyvinyl alcohol films reinforced by citric acid modified cellulose nanocrystals and silica aerogels.					
36241290	3	40	theme	compact	557:563	arg1	structure					565:573	a compact structure	555:573	a compact structure	555:573	SEM and FTIR results showed that MCNC and SA were uniformly dispersed in the PVA matrix and formed a compact structure.					
36241290	1	41	theme	citric	132:137	arg1	MCNC					177:180	MCNC	177:180	MCNC	177:180	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	41	theme	citric	132:137	arg1	aerogel					194:200	silica aerogel	187:200	silica aerogel (SA)	187:205	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	41	theme	citric	132:137	arg1	nanocrystals					163:174	citric acid modified cellulose nanocrystals	132:174	citric acid modified cellulose nanocrystals (MCNC)	132:181	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	41	theme	citric	132:137	arg1	agents					232:237	reinforcing agents	220:237	reinforcing agents	220:237	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	42	with	films	322:326	arg1	mechanical					343:352	mechanical	343:352	mechanical	343:352	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	42	with	films	322:326	arg1	properties					366:375	excellent mechanical and barrier properties	333:375	properties	366:375	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	4	43	theme	XRD	576:578	arg1	results					580:586	XRD results	576:586	XRD results	576:586	XRD results showed that there were physical interactions between the reinforcing agents and PVA, which improved the thermal stability of the film.					
36241290	0	44	theme	acid	59:62	arg1	nanocrystals					83:94	citric acid modified cellulose nanocrystals	52:94	citric acid modified cellulose nanocrystals	52:94	Properties of polyvinyl alcohol films reinforced by citric acid modified cellulose nanocrystals and silica aerogels.					
36241290	1	45	theme	different	279:287	arg1	proportions					289:299	different proportions	279:299	different proportions	279:299	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	2	46	theme	citric	402:407	arg1	acid					409:412	citric acid	402:412	citric acid	402:412	After modification with citric acid, MCNC successfully contained ester bonds.					
36241290	1	47	theme	acid	139:142	arg1	MCNC					177:180	MCNC	177:180	MCNC	177:180	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	47	theme	acid	139:142	arg1	aerogel					194:200	silica aerogel	187:200	silica aerogel (SA)	187:205	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	47	theme	acid	139:142	arg1	nanocrystals					163:174	citric acid modified cellulose nanocrystals	132:174	citric acid modified cellulose nanocrystals (MCNC)	132:181	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36241290	1	47	theme	acid	139:142	arg1	agents					232:237	reinforcing agents	220:237	reinforcing agents	220:237	In this study, citric acid modified cellulose nanocrystals (MCNC) and silica aerogel (SA) were used as reinforcing agents and added to polyvinyl alcohol (PVA) in different proportions to prepare composite films with excellent mechanical and barrier properties.					
36822290	4	0	theme	4:1	945:947	arg1	enzyme					935:940	enzyme	935:940	enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall	935:1052	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	0	theme	4:1	945:947	arg1	carrier					1079:1085	the proposed innovative carrier	1055:1085	the proposed innovative carrier for the fixation of lipase	1055:1112	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	1	theme	effect	681:686	arg1	rate					652:655	The grafting rate	639:655	The grafting rate of MCM-41 and shrinkage effect	639:686	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	2	2	theme	material	352:359	arg1	size					329:332	the pore size	320:332	the pore size of the mesoporous material	320:359	The prepared composite was grafted with silane coupling agent KH560 to decrease the pore size of the mesoporous material and prevent the loss of shed lipase molecules.					
36822290	4	3	theme	reaction	989:996	arg1	time					998:1001	reaction time	989:1001	reaction time of 3 h	989:1008	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	4	theme	crosslinking	826:837	arg1	agent					839:843	the crosslinking agent	822:843	the crosslinking agent	822:843	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	4	theme	crosslinking	826:837	arg1	conditions					858:867	the optimal conditions	846:867	the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore	846:1177	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	4	theme	crosslinking	826:837	arg1	glutaraldehyde					804:817	glutaraldehyde	804:817	glutaraldehyde	804:817	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	2	5	theme	mesoporous	341:350	arg1	material					352:359	the mesoporous material	337:359	the mesoporous material	337:359	The prepared composite was grafted with silane coupling agent KH560 to decrease the pore size of the mesoporous material and prevent the loss of shed lipase molecules.					
36822290	4	6	theme	3 h	1006:1008	arg1	temperature					1024:1034	reaction temperature	1015:1034	reaction temperature of 45 °C. Overall	1015:1052	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	6	theme	3 h	1006:1008	arg1	volume					974:979	glutaraldehyde solution volume	950:979	glutaraldehyde solution volume of 3 %	950:986	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	6	theme	3 h	1006:1008	arg1	time					998:1001	reaction time	989:1001	reaction time of 3 h	989:1008	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	6	theme	3 h	1006:1008	arg1	4:1					945:947	4:1	945:947	4:1	945:947	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	0	7	from	Immobilization	0:13	arg1	adjustment					78:87	pore size adjustment	68:87	pore size adjustment	68:87	Immobilization of lipase in chitosan-mesoporous silica material and pore size adjustment.					
36822290	0	7	from	Immobilization	0:13	arg1	material					55:62	chitosan-mesoporous silica material	28:62	chitosan-mesoporous silica material	28:62	Immobilization of lipase in chitosan-mesoporous silica material and pore size adjustment.					
36822290	4	8	theme	free	1155:1158	arg1	enzyme					1160:1165	the free enzyme	1151:1165	the free enzyme in the pore	1151:1177	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	2	9	theme	agent	296:300	arg1	KH560					302:306	silane coupling agent KH560	280:306	silane coupling agent KH560	280:306	The prepared composite was grafted with silane coupling agent KH560 to decrease the pore size of the mesoporous material and prevent the loss of shed lipase molecules.					
36822290	4	10	theme	grafting	643:650	arg1	rate					652:655	The grafting rate	639:655	The grafting rate of MCM-41 and shrinkage effect	639:686	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	3	11	theme	fixed	565:569	arg1	lipase					558:563	the lipase	554:563	the lipase fixed in the CTS-MCM-41 carrier	554:595	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	5	12	theme	large-scale	1241:1251	arg1	production					1264:1273	large-scale industrial production	1241:1273	large-scale industrial production	1241:1273	Moreover, the efficient miniature lipase reactor can promote large-scale industrial production.					
36822290	2	13	theme	coupling	287:294	arg1	KH560					302:306	silane coupling agent KH560	280:306	silane coupling agent KH560	280:306	The prepared composite was grafted with silane coupling agent KH560 to decrease the pore size of the mesoporous material and prevent the loss of shed lipase molecules.					
36822290	4	14	theme	proposed	1059:1066	arg1	enzyme					935:940	enzyme	935:940	enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall	935:1052	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	14	theme	proposed	1059:1066	arg1	carrier					1079:1085	the proposed innovative carrier	1055:1085	the proposed innovative carrier for the fixation of lipase	1055:1112	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	15	theme	shrinkage	671:679	arg1	effect					681:686	shrinkage effect	671:686	shrinkage effect	671:686	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	5	16	theme	industrial	1253:1262	arg1	production					1264:1273	large-scale industrial production	1241:1273	large-scale industrial production	1241:1273	Moreover, the efficient miniature lipase reactor can promote large-scale industrial production.					
36822290	2	17	theme	silane	280:285	arg1	KH560					302:306	silane coupling agent KH560	280:306	silane coupling agent KH560	280:306	The prepared composite was grafted with silane coupling agent KH560 to decrease the pore size of the mesoporous material and prevent the loss of shed lipase molecules.					
36822290	4	18	theme	temperature	1024:1034	arg1	enzyme					935:940	enzyme	935:940	enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall	935:1052	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	18	theme	temperature	1024:1034	arg1	carrier					1079:1085	the proposed innovative carrier	1055:1085	the proposed innovative carrier for the fixation of lipase	1055:1112	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	2	19	theme	shed	385:388	arg1	molecules					397:405	shed lipase molecules	385:405	shed lipase molecules	385:405	The prepared composite was grafted with silane coupling agent KH560 to decrease the pore size of the mesoporous material and prevent the loss of shed lipase molecules.					
36822290	4	20	theme	glutaraldehyde	950:963	arg1	volume					974:979	glutaraldehyde solution volume	950:979	glutaraldehyde solution volume of 3 %	950:986	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	3	21	theme	residual	533:540	arg1	activity					542:549	the residual activity	529:549	the residual activity of the lipase fixed in the CTS-MCM-41 carrier	529:595	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	3	21	theme	residual	533:540	arg1	%					604:604	85 %	601:604	85 %	601:604	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	4	22	theme	carrier	924:930	arg1	ratio					911:915	a mass ratio	904:915	a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase	904:1112	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	22	theme	carrier	924:930	arg1	stable					1117:1122	stable	1117:1122	stable	1117:1122	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	1	23	theme	Mesoporous	90:99	arg1	silica					101:106	Mesoporous silica MCM-41	90:113	Mesoporous silica MCM-41	90:113	Mesoporous silica MCM-41 was modified by carboxyl groups and assembled with chitosan to produce a novel nanocarrier for the immobilization of lipase.					
36822290	4	24	theme	innovative	1068:1077	arg1	enzyme					935:940	enzyme	935:940	enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall	935:1052	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	24	theme	innovative	1068:1077	arg1	carrier					1079:1085	the proposed innovative carrier	1055:1085	the proposed innovative carrier for the fixation of lipase	1055:1112	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	25	theme	Overall	1046:1052	arg1	45 °C.					1039:1044	45 °C. Overall	1039:1052	45 °C. Overall	1039:1052	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	26	theme	45 °C.	1039:1044	arg1	temperature					1024:1034	reaction temperature	1015:1034	reaction temperature of 45 °C. Overall	1015:1052	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	26	theme	45 °C.	1039:1044	arg1	volume					974:979	glutaraldehyde solution volume	950:979	glutaraldehyde solution volume of 3 %	950:986	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	26	theme	45 °C.	1039:1044	arg1	time					998:1001	reaction time	989:1001	reaction time of 3 h	989:1008	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	26	theme	45 °C.	1039:1044	arg1	4:1					945:947	4:1	945:947	4:1	945:947	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	1	27	theme	novel	188:192	arg1	nanocarrier					194:204	a novel nanocarrier	186:204	a novel nanocarrier for the immobilization of lipase	186:237	Mesoporous silica MCM-41 was modified by carboxyl groups and assembled with chitosan to produce a novel nanocarrier for the immobilization of lipase.					
36822290	0	28	theme	lipase	18:23	arg1	Immobilization					0:13	Immobilization	0:13	Immobilization of lipase in chitosan-mesoporous silica material and pore size adjustment.	0:88	Immobilization of lipase in chitosan-mesoporous silica material and pore size adjustment.					
36822290	4	29	from	enzyme	1160:1165	arg1	pore					1174:1177	the pore	1170:1177	the pore	1170:1177	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	3	30	theme	lipase	558:563	arg1	activity					542:549	the residual activity	529:549	the residual activity of the lipase fixed in the CTS-MCM-41 carrier	529:595	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	3	30	theme	lipase	558:563	arg1	%					604:604	85 %	601:604	85 %	601:604	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	3	31	from	carrier	589:595	arg1	fixed					565:569	fixed	565:569	fixed	565:569	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	0	32	theme	silica	48:53	arg1	material					55:62	chitosan-mesoporous silica material	28:62	chitosan-mesoporous silica material	28:62	Immobilization of lipase in chitosan-mesoporous silica material and pore size adjustment.					
36822290	4	33	theme	mass	906:909	arg1	ratio					911:915	a mass ratio	904:915	a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase	904:1112	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	33	theme	mass	906:909	arg1	stable					1117:1122	stable	1117:1122	stable	1117:1122	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	1	34	mod	modified	119:126	arg1	silica					101:106	Mesoporous silica MCM-41	90:113	Mesoporous silica MCM-41	90:113	Mesoporous silica MCM-41 was modified by carboxyl groups and assembled with chitosan to produce a novel nanocarrier for the immobilization of lipase.					
36822290	1	34	mod	modified	119:126	arg3	groups					140:145	carboxyl groups	131:145	carboxyl groups	131:145	Mesoporous silica MCM-41 was modified by carboxyl groups and assembled with chitosan to produce a novel nanocarrier for the immobilization of lipase.					
36822290	2	35	theme	molecules	397:405	arg1	loss					377:380	the loss	373:380	the loss of shed lipase molecules	373:405	The prepared composite was grafted with silane coupling agent KH560 to decrease the pore size of the mesoporous material and prevent the loss of shed lipase molecules.					
36822290	0	36	theme	chitosan-mesoporous	28:46	arg1	material					55:62	chitosan-mesoporous silica material	28:62	chitosan-mesoporous silica material	28:62	Immobilization of lipase in chitosan-mesoporous silica material and pore size adjustment.					
36822290	3	37	theme	repeated	618:625	arg1	cycles					631:636	seven repeated use cycles	612:636	seven repeated use cycles	612:636	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	4	38	from	70 °C.	791:796	arg1	4 h					784:786	4 h	784:786	4 h at 70 °C.	784:796	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	2	39	theme	lipase	390:395	arg1	molecules					397:405	shed lipase molecules	385:405	shed lipase molecules	385:405	The prepared composite was grafted with silane coupling agent KH560 to decrease the pore size of the mesoporous material and prevent the loss of shed lipase molecules.					
36822290	4	40	theme	volume	974:979	arg1	enzyme					935:940	enzyme	935:940	enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall	935:1052	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	40	theme	volume	974:979	arg1	carrier					1079:1085	the proposed innovative carrier	1055:1085	the proposed innovative carrier for the fixation of lipase	1055:1112	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	3	41	theme	use	627:629	arg1	cycles					631:636	seven repeated use cycles	612:636	seven repeated use cycles	612:636	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	4	42	theme	lipase	1107:1112	arg1	fixation					1095:1102	the fixation	1091:1102	the fixation of lipase	1091:1112	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	43	theme	solution	965:972	arg1	volume					974:979	glutaraldehyde solution volume	950:979	glutaraldehyde solution volume of 3 %	950:986	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	44	theme	optimal	850:856	arg1	agent					839:843	the crosslinking agent	822:843	the crosslinking agent	822:843	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	44	theme	optimal	850:856	arg1	conditions					858:867	the optimal conditions	846:867	the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore	846:1177	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	5	45	theme	lipase	1214:1219	arg1	reactor					1221:1227	the efficient miniature lipase reactor	1190:1227	the efficient miniature lipase reactor	1190:1227	Moreover, the efficient miniature lipase reactor can promote large-scale industrial production.					
36822290	3	46	theme	CTS-MCM-41	578:587	arg1	carrier					589:595	the CTS-MCM-41 carrier	574:595	the CTS-MCM-41 carrier	574:595	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	3	47	theme	related	509:515	arg1	parameters					517:526	related parameters	509:526	related parameters	509:526	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	4	48	theme	enzyme	873:878	arg1	immobilization					880:893	enzyme immobilization	873:893	enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore	873:1177	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	49	theme	%	986:986	arg1	temperature					1024:1034	reaction temperature	1015:1034	reaction temperature of 45 °C. Overall	1015:1052	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	49	theme	%	986:986	arg1	volume					974:979	glutaraldehyde solution volume	950:979	glutaraldehyde solution volume of 3 %	950:986	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	49	theme	%	986:986	arg1	time					998:1001	reaction time	989:1001	reaction time of 3 h	989:1008	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	49	theme	%	986:986	arg1	4:1					945:947	4:1	945:947	4:1	945:947	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	1	50	theme	carboxyl	131:138	arg1	groups					140:145	carboxyl groups	131:145	carboxyl groups	131:145	Mesoporous silica MCM-41 was modified by carboxyl groups and assembled with chitosan to produce a novel nanocarrier for the immobilization of lipase.					
36822290	5	51	theme	efficient	1194:1202	arg1	reactor					1221:1227	the efficient miniature lipase reactor	1190:1227	the efficient miniature lipase reactor	1190:1227	Moreover, the efficient miniature lipase reactor can promote large-scale industrial production.					
36822290	4	52	theme	KH560	729:733	arg1	%					743:743	5.0 %	739:743	5.0 %	739:743	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	52	theme	KH560	729:733	arg1	concentration					712:724	the concentration	708:724	the concentration of KH560	708:733	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	2	53	theme	pore	324:327	arg1	size					329:332	the pore size	320:332	the pore size of the mesoporous material	320:359	The prepared composite was grafted with silane coupling agent KH560 to decrease the pore size of the mesoporous material and prevent the loss of shed lipase molecules.					
36822290	3	54	from	fixed	565:569	arg1	carrier					589:595	the CTS-MCM-41 carrier	574:595	the CTS-MCM-41 carrier	574:595	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	1	55	theme	lipase	232:237	arg1	immobilization					214:227	the immobilization	210:227	the immobilization of lipase	210:237	Mesoporous silica MCM-41 was modified by carboxyl groups and assembled with chitosan to produce a novel nanocarrier for the immobilization of lipase.					
36822290	0	56	theme	size	73:76	arg1	adjustment					78:87	pore size adjustment	68:87	pore size adjustment	68:87	Immobilization of lipase in chitosan-mesoporous silica material and pore size adjustment.					
36822290	3	57	theme	material	449:456	arg1	characterization					425:440	the characterization	421:440	the characterization of the material before and after modification and determination of related parameters	421:526	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	3	58	theme	parameters	517:526	arg1	determination					492:504	determination	492:504	determination of related parameters	492:526	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	3	58	theme	parameters	517:526	arg1	modification					475:486	modification	475:486	modification	475:486	According to the characterization of the material before and after modification and determination of related parameters, the residual activity of the lipase fixed in the CTS-MCM-41 carrier was 85 % after seven repeated use cycles.					
36822290	4	59	theme	reaction	1015:1022	arg1	temperature					1024:1034	reaction temperature	1015:1034	reaction temperature of 45 °C. Overall	1015:1052	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	0	60	theme	pore	68:71	arg1	adjustment					78:87	pore size adjustment	68:87	pore size adjustment	68:87	Immobilization of lipase in chitosan-mesoporous silica material and pore size adjustment.					
36822290	2	61	theme	prepared	244:251	arg1	composite					253:261	The prepared composite	240:261	The prepared composite	240:261	The prepared composite was grafted with silane coupling agent KH560 to decrease the pore size of the mesoporous material and prevent the loss of shed lipase molecules.					
36822290	5	62	theme	miniature	1204:1212	arg1	reactor					1221:1227	the efficient miniature lipase reactor	1190:1227	the efficient miniature lipase reactor	1190:1227	Moreover, the efficient miniature lipase reactor can promote large-scale industrial production.					
36822290	4	63	theme	MCM-41	660:665	arg1	rate					652:655	The grafting rate	639:655	The grafting rate of MCM-41 and shrinkage effect	639:686	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	64	theme	time	998:1001	arg1	enzyme					935:940	enzyme	935:940	enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall	935:1052	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
36822290	4	64	theme	time	998:1001	arg1	carrier					1079:1085	the proposed innovative carrier	1055:1085	the proposed innovative carrier for the fixation of lipase	1055:1112	The grafting rate of MCM-41 and shrinkage effect were maximized when the concentration of KH560 was 5.0 %, and the modification was performed at 4 h at 70 °C. Using glutaraldehyde as the crosslinking agent, the optimal conditions for enzyme immobilization involved a mass ratio of the carrier to enzyme of 4:1, glutaraldehyde solution volume of 3 %, reaction time of 3 h, and reaction temperature of 45 °C. Overall, the proposed innovative carrier for the fixation of lipase is stable and can physically control the free enzyme in the pore.					
35337504	3	0	theme	Rorγt+	735:740	arg1	frequencies					760:770	Rorγt+ regulatory T-cell frequencies	735:770	Rorγt+ regulatory T-cell frequencies	735:770	After 4 weeks of supplementation with those pectins, Th1 and Th2 frequencies returned to control levels, whereas Rorγt+ regulatory T-cell frequencies increased.					
35337504	1	1	theme	fermentation	216:227	arg1	profiles					229:236	fermentation profiles	216:236	fermentation profiles	216:236	Pectins are dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles, but how this is influenced by the degree of methyl-esterification (DM) and degree-of-blockiness (DB) of pectin is unknown.					
35337504	0	2	theme	pectin	68:73	arg1	impact					58:63	the impact	54:63	the impact of pectin on intestinal T cells, microbiota, and Ahr activation	54:127	The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation.					
35337504	2	3	from	supplementation	388:402	arg1	dose					470:473	a low dose	464:473	a low dose	464:473	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	2	4	theme	Th2	534:536	arg1	cells					538:542	intestinal T-helper (Th)1 and Th2 cells	504:542	cells	538:542	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	2	5	from	1 week	550:555	arg1	mice					586:589	mice	586:589	mice	586:589	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	3	6	theme	Th2	683:685	arg1	frequencies					687:697	Th1 and Th2 frequencies	675:697	Th1 and Th2 frequencies	675:697	After 4 weeks of supplementation with those pectins, Th1 and Th2 frequencies returned to control levels, whereas Rorγt+ regulatory T-cell frequencies increased.					
35337504	0	7	theme	T	89:89	arg1	cells					91:95	intestinal T cells	78:95	intestinal T cells	78:95	The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation.					
35337504	5	8	from	increase	1032:1039	arg1	components					1081:1090	Aryl-hydrocarbon-receptor-activating components	1044:1090	Aryl-hydrocarbon-receptor-activating components	1044:1090	T-cell-modulating effects were not short-chain-fatty acid-dependent, but rather through an increase in Aryl-hydrocarbon-receptor-activating components.					
35337504	4	9	theme	other	926:930	arg1	pectins					932:938	the other pectins	922:938	the other pectins	922:938	These structure-dependent effects could derive from induced shifts in microbiota composition that differed between DM18(low-DB) pectin and the other pectins.					
35337504	2	10	from	dose	470:473	arg1	pectins					453:459	DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins	407:459	DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose	407:473	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	2	10	from	dose	470:473	arg1	supplementation					388:402	supplementation	388:402	supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose	388:473	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	0	11	theme	intestinal	78:87	arg1	cells					91:95	intestinal T cells	78:95	intestinal T cells	78:95	The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation.					
35337504	6	12	theme	intestinal	1166:1175	arg1	cell-immunity					1179:1191	intestinal T cell-immunity	1166:1191	intestinal T cell-immunity in mice	1166:1199	Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.					
35337504	3	13	theme	Th1	675:677	arg1	frequencies					687:697	Th1 and Th2 frequencies	675:697	Th1 and Th2 frequencies	675:697	After 4 weeks of supplementation with those pectins, Th1 and Th2 frequencies returned to control levels, whereas Rorγt+ regulatory T-cell frequencies increased.					
35337504	4	14	theme	induced	835:841	arg1	shifts					843:848	induced shifts	835:848	induced shifts in microbiota composition that differed between DM18(low-DB) pectin and the other pectins	835:938	These structure-dependent effects could derive from induced shifts in microbiota composition that differed between DM18(low-DB) pectin and the other pectins.					
35337504	2	15	theme	Th	525:526	arg1	frequencies					489:499	the frequencies	485:499	the frequencies of intestinal T-helper (Th)1 and Th2 cells	485:542	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	1	16	theme	dietary	142:148	arg1	Pectins					130:136	Pectins	130:136	Pectins	130:136	Pectins are dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles, but how this is influenced by the degree of methyl-esterification (DM) and degree-of-blockiness (DB) of pectin is unknown.					
35337504	1	16	theme	dietary	142:148	arg1	fibres					150:155	dietary fibres	142:155	dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles	142:236	Pectins are dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles, but how this is influenced by the degree of methyl-esterification (DM) and degree-of-blockiness (DB) of pectin is unknown.					
35337504	6	17	from	impact	1156:1161	arg1	cell-immunity					1179:1191	intestinal T cell-immunity	1166:1191	intestinal T cell-immunity in mice	1166:1199	Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.					
35337504	3	18	theme	regulatory	742:751	arg1	frequencies					760:770	Rorγt+ regulatory T-cell frequencies	735:770	Rorγt+ regulatory T-cell frequencies	735:770	After 4 weeks of supplementation with those pectins, Th1 and Th2 frequencies returned to control levels, whereas Rorγt+ regulatory T-cell frequencies increased.					
35337504	4	19	theme	low-DB	903:908	arg1	pectin					911:916	DM18(low-DB) pectin	898:916	DM18(low-DB) pectin	898:916	These structure-dependent effects could derive from induced shifts in microbiota composition that differed between DM18(low-DB) pectin and the other pectins.					
35337504	4	20	theme	microbiota	853:862	arg1	composition					864:874	microbiota composition	853:874	microbiota composition that differed between DM18(low-DB) pectin and the other pectins	853:938	These structure-dependent effects could derive from induced shifts in microbiota composition that differed between DM18(low-DB) pectin and the other pectins.					
35337504	2	21	theme	T-helper	515:522	arg1	Th					525:526	intestinal T-helper (Th)1 and Th2 cells	504:542	Th	525:526	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	3	22	theme	T-cell	753:758	arg1	frequencies					760:770	Rorγt+ regulatory T-cell frequencies	735:770	Rorγt+ regulatory T-cell frequencies	735:770	After 4 weeks of supplementation with those pectins, Th1 and Th2 frequencies returned to control levels, whereas Rorγt+ regulatory T-cell frequencies increased.					
35337504	2	23	theme	intestinal	504:513	arg1	Th					525:526	intestinal T-helper (Th)1 and Th2 cells	504:542	Th	525:526	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	4	24	theme	DM18	898:901	arg1	pectin					911:916	DM18(low-DB) pectin	898:916	DM18(low-DB) pectin	898:916	These structure-dependent effects could derive from induced shifts in microbiota composition that differed between DM18(low-DB) pectin and the other pectins.					
35337504	6	25	contain	have	1148:1151	arg2	impact					1156:1161	an impact	1153:1161	an impact on intestinal T cell-immunity in mice	1153:1199	Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.					
35337504	6	25	contain	have	1148:1151	arg1	pectins					1099:1105	pectins	1099:1105	pectins with a specific combination of DM and DB	1099:1146	Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.					
35337504	2	26	theme	pectins	453:459	arg1	supplementation					388:402	supplementation	388:402	supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose	388:473	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	2	27	theme	cells	538:542	arg1	frequencies					489:499	the frequencies	485:499	the frequencies of intestinal T-helper (Th)1 and Th2 cells	485:542	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	1	28	theme	T	171:171	arg1	immunity					178:185	T cell immunity	171:185	T cell immunity	171:185	Pectins are dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles, but how this is influenced by the degree of methyl-esterification (DM) and degree-of-blockiness (DB) of pectin is unknown.					
35337504	6	29	with	pectins	1099:1105	arg1	combination					1123:1133	a specific combination	1112:1133	a specific combination of DM and DB	1112:1146	Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.					
35337504	6	30	theme	low	1225:1227	arg1	dose					1229:1232	a low dose	1223:1232	a low dose	1223:1232	Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.					
35337504	1	31	theme	cell	173:176	arg1	immunity					178:185	T cell immunity	171:185	T cell immunity	171:185	Pectins are dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles, but how this is influenced by the degree of methyl-esterification (DM) and degree-of-blockiness (DB) of pectin is unknown.					
35337504	0	32	theme	methyl-esters	30:42	arg1	distribution					14:25	distribution	14:25	distribution	14:25	The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation.					
35337504	0	32	theme	methyl-esters	30:42	arg1	level					4:8	level	4:8	level	4:8	The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation.					
35337504	0	33	theme	Ahr	114:116	arg1	activation					118:127	Ahr activation	114:127	Ahr activation	114:127	The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation.					
35337504	5	34	theme	T-cell-modulating	941:957	arg1	effects					959:965	T-cell-modulating effects	941:965	T-cell-modulating effects	941:965	T-cell-modulating effects were not short-chain-fatty acid-dependent, but rather through an increase in Aryl-hydrocarbon-receptor-activating components.					
35337504	6	35	theme	DB	1145:1146	arg1	combination					1123:1133	a specific combination	1112:1133	a specific combination of DM and DB	1112:1146	Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.					
35337504	4	36	theme	structure-dependent	789:807	arg1	effects					809:815	These structure-dependent effects	783:815	These structure-dependent effects	783:815	These structure-dependent effects could derive from induced shifts in microbiota composition that differed between DM18(low-DB) pectin and the other pectins.					
35337504	1	37	theme	pectin	343:348	arg1	degree-of-blockiness					314:333	degree-of-blockiness	314:333	degree-of-blockiness (DB) of pectin	314:348	Pectins are dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles, but how this is influenced by the degree of methyl-esterification (DM) and degree-of-blockiness (DB) of pectin is unknown.					
35337504	1	37	theme	pectin	343:348	arg1	DB					336:337	DB	336:337	DB	336:337	Pectins are dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles, but how this is influenced by the degree of methyl-esterification (DM) and degree-of-blockiness (DB) of pectin is unknown.					
35337504	2	38	theme	DM19	407:410	arg1	pectins					453:459	DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins	407:459	DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose	407:473	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	2	39	theme	DM43	439:442	arg1	pectins					453:459	DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins	407:459	DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose	407:473	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	0	40	from	impact	58:63	arg1	microbiota					98:107	microbiota	98:107	microbiota	98:107	The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation.					
35337504	0	40	from	impact	58:63	arg1	cells					91:95	intestinal T cells	78:95	intestinal T cells	78:95	The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation.					
35337504	0	40	from	impact	58:63	arg1	activation					118:127	Ahr activation	114:127	Ahr activation	114:127	The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation.					
35337504	6	41	theme	DM	1138:1139	arg1	combination					1123:1133	a specific combination	1112:1133	a specific combination of DM and DB	1112:1146	Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.					
35337504	5	42	theme	Aryl-hydrocarbon-receptor-activating	1044:1079	arg1	components					1081:1090	Aryl-hydrocarbon-receptor-activating components	1044:1090	Aryl-hydrocarbon-receptor-activating components	1044:1090	T-cell-modulating effects were not short-chain-fatty acid-dependent, but rather through an increase in Aryl-hydrocarbon-receptor-activating components.					
35337504	2	43	theme	low	466:468	arg1	dose					470:473	a low dose	464:473	a low dose	464:473	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	2	44	theme	supplementation	567:581	arg1	1 week					550:555	1 week	550:555	1 week of pectin supplementation in mice	550:589	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	6	45	theme	T	1177:1177	arg1	cell-immunity					1179:1191	intestinal T cell-immunity	1166:1191	intestinal T cell-immunity in mice	1166:1199	Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.					
35337504	2	46	theme	pectin	560:565	arg1	supplementation					567:581	pectin supplementation	560:581	pectin supplementation	560:581	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	3	47	theme	control	711:717	arg1	levels					719:724	control levels	711:724	control levels	711:724	After 4 weeks of supplementation with those pectins, Th1 and Th2 frequencies returned to control levels, whereas Rorγt+ regulatory T-cell frequencies increased.					
35337504	1	48	theme	microbiota	188:197	arg1	composition					199:209	microbiota composition	188:209	microbiota composition	188:209	Pectins are dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles, but how this is influenced by the degree of methyl-esterification (DM) and degree-of-blockiness (DB) of pectin is unknown.					
35337504	6	49	theme	specific	1114:1121	arg1	combination					1123:1133	a specific combination	1112:1133	a specific combination of DM and DB	1112:1146	Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.					
35337504	3	50	with	supplementation	639:653	arg1	pectins					666:672	those pectins	660:672	those pectins	660:672	After 4 weeks of supplementation with those pectins, Th1 and Th2 frequencies returned to control levels, whereas Rorγt+ regulatory T-cell frequencies increased.					
35337504	0	51	dep	level	4:8	arg1	The					0:2	The	0:2	The	0:2	The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation.					
35337504	3	52	theme	supplementation	639:653	arg1	4 weeks					628:634	4 weeks	628:634	4 weeks of supplementation with those pectins	628:672	After 4 weeks of supplementation with those pectins, Th1 and Th2 frequencies returned to control levels, whereas Rorγt+ regulatory T-cell frequencies increased.					
35337504	2	53	theme	DM49	422:425	arg1	pectins					453:459	DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins	407:459	DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose	407:473	Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not.					
35337504	1	54	theme	methyl-esterification	283:303	arg1	degree					273:278	the degree	269:278	the degree of methyl-esterification (DM)	269:308	Pectins are dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles, but how this is influenced by the degree of methyl-esterification (DM) and degree-of-blockiness (DB) of pectin is unknown.					
35337504	6	55	from	cell-immunity	1179:1191	arg1	mice					1196:1199	mice	1196:1199	mice	1196:1199	Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.					
35337504	4	56	from	shifts	843:848	arg1	composition					864:874	microbiota composition	853:874	microbiota composition that differed between DM18(low-DB) pectin and the other pectins	853:938	These structure-dependent effects could derive from induced shifts in microbiota composition that differed between DM18(low-DB) pectin and the other pectins.					
35104515	5	0	theme	@	711:711	arg1	composites					721:730	new MXene@Fe3O4@CS composites	702:730	new MXene@Fe3O4@CS composites	702:730	MXene can provide suitable support for both CS and Fe3O4 to construct new MXene@Fe3O4@CS composites.					
35104515	7	1	theme	X-ray	928:932	arg1	XPS					962:964	XPS	962:964	XPS	962:964	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	7	1	theme	X-ray	928:932	arg1	spectroscopy					948:959	X-ray photoelectron spectroscopy	928:959	X-ray photoelectron spectroscopy (XPS)	928:965	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	5	2	theme	Fe3O4	712:716	arg1	composites					721:730	new MXene@Fe3O4@CS composites	702:730	new MXene@Fe3O4@CS composites	702:730	MXene can provide suitable support for both CS and Fe3O4 to construct new MXene@Fe3O4@CS composites.					
35104515	8	3	theme	adsorption	1110:1119	arg1	kinetics					1121:1128	the adsorption kinetics	1106:1128	the adsorption kinetics	1106:1128	According to the batch experiments, the adsorption kinetics were found to predominantly follow quasi-secondary rate kinetics.					
35104515	3	4	theme	surface	458:464	arg1	functionalization					466:482	surface functionalization	458:482	surface functionalization	458:482	Nano-Fe3O4 has the advantages of easy magnetic separation and surface functionalization, which can improve the efficiency as well as selectivity of separation.					
35104515	0	5	theme	efficient	91:99	arg1	adsorption					101:110	highly efficient adsorption	84:110	highly efficient adsorption	84:110	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.					
35104515	7	6	theme	Scanning	852:859	arg1	microscopy					870:879	Scanning electron microscopy	852:879	Scanning electron microscopy (SEM)	852:885	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	7	6	theme	Scanning	852:859	arg1	SEM					882:884	SEM	882:884	SEM	882:884	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	1	7	from	presence	147:154	arg1	surface					200:206	the surface	196:206	the surface	196:206	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	10	8	theme	endothermic	1284:1294	arg1	process					1260:1266	The adsorption process	1245:1266	The adsorption process	1245:1266	The adsorption process was found to be endothermic, entropy-driven, and thermodynamically spontaneous process.					
35104515	10	8	theme	endothermic	1284:1294	arg1	process					1347:1353	endothermic, entropy-driven, and thermodynamically spontaneous process	1284:1353	endothermic, entropy-driven, and thermodynamically spontaneous process	1284:1353	The adsorption process was found to be endothermic, entropy-driven, and thermodynamically spontaneous process.					
35104515	9	9	theme	Langmuir	1220:1227	arg1	model					1238:1242	Langmuir isotherm model	1220:1242	Langmuir isotherm model	1220:1242	The adsorption followed Langmuir isotherm model.					
35104515	7	10	theme	FT-IR	1000:1004	arg1	spectroscopy					1007:1018	Infrared (FT-IR) spectroscopy	990:1018	Infrared (FT-IR) spectroscopy	990:1018	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	7	11	theme	electron	861:868	arg1	microscopy					870:879	Scanning electron microscopy	852:879	Scanning electron microscopy (SEM)	852:885	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	7	11	theme	electron	861:868	arg1	SEM					882:884	SEM	882:884	SEM	882:884	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	8	12	theme	quasi-secondary	1165:1179	arg1	kinetics					1186:1193	quasi-secondary rate kinetics	1165:1193	quasi-secondary rate kinetics	1165:1193	According to the batch experiments, the adsorption kinetics were found to predominantly follow quasi-secondary rate kinetics.					
35104515	9	13	theme	isotherm	1229:1236	arg1	model					1238:1242	Langmuir isotherm model	1220:1242	Langmuir isotherm model	1220:1242	The adsorption followed Langmuir isotherm model.					
35104515	8	14	theme	batch	1087:1091	arg1	experiments					1093:1103	the batch experiments	1083:1103	the batch experiments	1083:1103	According to the batch experiments, the adsorption kinetics were found to predominantly follow quasi-secondary rate kinetics.					
35104515	4	15	theme	adsorption	611:620	arg1	capacity					622:629	the adsorption capacity	607:629	the adsorption capacity	607:629	However, its tendency for agglomeration can reduce the adsorption capacity.					
35104515	0	16	theme	MXene-supported	43:57	arg1	nanospheres					68:78	MXene-supported Fe3O4@CS nanospheres	43:78	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.	0:134	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.					
35104515	1	17	attach	presence	147:154	arg1	surface					200:206	the surface	196:206	the surface	196:206	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	1	17	attach	presence	147:154	arg2	groups					186:191	several hydroxyl and amino groups	159:191	groups	186:191	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	1	17	attach	presence	147:154	arg2	hydroxyl					167:174	several hydroxyl and amino groups	159:191	hydroxyl	167:174	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	7	18	theme	Infrared	990:997	arg1	spectroscopy					1007:1018	Infrared (FT-IR) spectroscopy	990:1018	Infrared (FT-IR) spectroscopy	990:1018	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	6	19	theme	CSmagnetic	760:769	arg1	nanosphere					771:780	MXene@Fe3O4@CSmagnetic nanosphere	748:780	MXene@Fe3O4@CSmagnetic nanosphere	748:780	In this study, MXene@Fe3O4@CSmagnetic nanosphere was synthesized by ultrasonic self-assembly to remove Congo red (CR).					
35104515	1	20	theme	several	159:165	arg1	hydroxyl					167:174	several hydroxyl and amino groups	159:191	hydroxyl	167:174	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	1	21	theme	industrial	320:329	arg1	wastewater					331:340	different industrial wastewater	310:340	different industrial wastewater	310:340	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	5	22	theme	new	702:704	arg1	composites					721:730	new MXene@Fe3O4@CS composites	702:730	new MXene@Fe3O4@CS composites	702:730	MXene can provide suitable support for both CS and Fe3O4 to construct new MXene@Fe3O4@CS composites.					
35104515	11	23	theme	adsorption	1360:1369	arg1	capacity					1371:1378	The adsorption capacity	1356:1378	The adsorption capacity for CR	1356:1385	The adsorption capacity for CR was estimated as 620.22 mg·g-1.					
35104515	11	23	theme	adsorption	1360:1369	arg1	mg·g-1					1411:1416	620.22 mg·g-1	1404:1416	620.22 mg·g-1	1404:1416	The adsorption capacity for CR was estimated as 620.22 mg·g-1.					
35104515	1	24	theme	hydroxyl	167:174	arg1	presence					147:154	the presence	143:154	the presence of several hydroxyl and amino groups on the surface	143:206	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	10	25	theme	spontaneous	1335:1345	arg1	process					1260:1266	The adsorption process	1245:1266	The adsorption process	1245:1266	The adsorption process was found to be endothermic, entropy-driven, and thermodynamically spontaneous process.					
35104515	10	25	theme	spontaneous	1335:1345	arg1	process					1347:1353	endothermic, entropy-driven, and thermodynamically spontaneous process	1284:1353	endothermic, entropy-driven, and thermodynamically spontaneous process	1284:1353	The adsorption process was found to be endothermic, entropy-driven, and thermodynamically spontaneous process.					
35104515	1	26	from	dyes	302:305	arg1	wastewater					331:340	different industrial wastewater	310:340	different industrial wastewater	310:340	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	10	27	theme	thermodynamically	1317:1333	arg1	process					1260:1266	The adsorption process	1245:1266	The adsorption process	1245:1266	The adsorption process was found to be endothermic, entropy-driven, and thermodynamically spontaneous process.					
35104515	10	27	theme	thermodynamically	1317:1333	arg1	process					1347:1353	endothermic, entropy-driven, and thermodynamically spontaneous process	1284:1353	endothermic, entropy-driven, and thermodynamically spontaneous process	1284:1353	The adsorption process was found to be endothermic, entropy-driven, and thermodynamically spontaneous process.					
35104515	1	28	theme	amino	180:184	arg1	groups					186:191	several hydroxyl and amino groups	159:191	groups	186:191	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	0	29	theme	3D	15:16	arg1	nanocomposites					27:40	3D magnetic nanocomposites	15:40	3D magnetic nanocomposites	15:40	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.					
35104515	3	30	contain	has	407:409	arg2	advantages					415:424	the advantages	411:424	the advantages	411:424	Nano-Fe3O4 has the advantages of easy magnetic separation and surface functionalization, which can improve the efficiency as well as selectivity of separation.					
35104515	3	30	contain	has	407:409	arg1	Nano-Fe3O4					396:405	Nano-Fe3O4	396:405	Nano-Fe3O4	396:405	Nano-Fe3O4 has the advantages of easy magnetic separation and surface functionalization, which can improve the efficiency as well as selectivity of separation.					
35104515	1	31	theme	groups	186:191	arg1	presence					147:154	the presence	143:154	the presence of several hydroxyl and amino groups on the surface	143:206	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	5	32	theme	@	717:717	arg1	composites					721:730	new MXene@Fe3O4@CS composites	702:730	new MXene@Fe3O4@CS composites	702:730	MXene can provide suitable support for both CS and Fe3O4 to construct new MXene@Fe3O4@CS composites.					
35104515	6	33	theme	ultrasonic	801:810	arg1	self-assembly					812:824	ultrasonic self-assembly	801:824	ultrasonic self-assembly	801:824	In this study, MXene@Fe3O4@CSmagnetic nanosphere was synthesized by ultrasonic self-assembly to remove Congo red (CR).					
35104515	3	34	theme	separation	544:553	arg1	selectivity					529:539	selectivity	529:539	the efficiency as well as selectivity of separation	503:553	Nano-Fe3O4 has the advantages of easy magnetic separation and surface functionalization, which can improve the efficiency as well as selectivity of separation.					
35104515	3	34	theme	separation	544:553	arg1	efficiency					507:516	the efficiency	503:516	the efficiency as well as selectivity of separation	503:553	Nano-Fe3O4 has the advantages of easy magnetic separation and surface functionalization, which can improve the efficiency as well as selectivity of separation.					
35104515	0	35	theme	nanocomposites	27:40	arg1	Application					0:10	Application	0:10	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.	0:134	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.					
35104515	1	36	theme	potential	249:257	arg1	chitosan					209:216	chitosan	209:216	chitosan (CS)	209:221	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	1	36	theme	potential	249:257	arg1	candidate					259:267	a potential candidate	247:267	a potential candidate to solve the pollution caused by dyes in different industrial wastewater	247:340	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	0	37	theme	dyes	130:133	arg1	separation					116:125	separation	116:125	separation of dyes	116:133	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.					
35104515	0	37	theme	dyes	130:133	arg1	adsorption					101:110	highly efficient adsorption	84:110	highly efficient adsorption	84:110	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.					
35104515	0	38	theme	magnetic	18:25	arg1	nanocomposites					27:40	3D magnetic nanocomposites	15:40	3D magnetic nanocomposites	15:40	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.					
35104515	8	39	theme	rate	1181:1184	arg1	kinetics					1186:1193	quasi-secondary rate kinetics	1165:1193	quasi-secondary rate kinetics	1165:1193	According to the batch experiments, the adsorption kinetics were found to predominantly follow quasi-secondary rate kinetics.					
35104515	0	40	theme	Fe3O4	59:63	arg1	nanospheres					68:78	MXene-supported Fe3O4@CS nanospheres	43:78	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.	0:134	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.					
35104515	7	41	theme	photoelectron	934:946	arg1	XPS					962:964	XPS	962:964	XPS	962:964	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	7	41	theme	photoelectron	934:946	arg1	spectroscopy					948:959	X-ray photoelectron spectroscopy	928:959	X-ray photoelectron spectroscopy (XPS)	928:965	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	5	42	theme	MXene	706:710	arg1	composites					721:730	new MXene@Fe3O4@CS composites	702:730	new MXene@Fe3O4@CS composites	702:730	MXene can provide suitable support for both CS and Fe3O4 to construct new MXene@Fe3O4@CS composites.					
35104515	1	43	theme	different	310:318	arg1	wastewater					331:340	different industrial wastewater	310:340	different industrial wastewater	310:340	Due to the presence of several hydroxyl and amino groups on the surface, chitosan (CS) has been reported to be a potential candidate to solve the pollution caused by dyes in different industrial wastewater.					
35104515	3	44	theme	magnetic	434:441	arg1	separation					443:452	easy magnetic separation	429:452	easy magnetic separation	429:452	Nano-Fe3O4 has the advantages of easy magnetic separation and surface functionalization, which can improve the efficiency as well as selectivity of separation.					
35104515	2	45	theme	recycling	378:386	arg1	issues					388:393	the recycling issues	374:393	the recycling issues	374:393	However, it is associated with the recycling issues.					
35104515	6	46	theme	@	759:759	arg1	nanosphere					771:780	MXene@Fe3O4@CSmagnetic nanosphere	748:780	MXene@Fe3O4@CSmagnetic nanosphere	748:780	In this study, MXene@Fe3O4@CSmagnetic nanosphere was synthesized by ultrasonic self-assembly to remove Congo red (CR).					
35104515	10	47	theme	adsorption	1249:1258	arg1	process					1260:1266	The adsorption process	1245:1266	The adsorption process	1245:1266	The adsorption process was found to be endothermic, entropy-driven, and thermodynamically spontaneous process.					
35104515	10	47	theme	adsorption	1249:1258	arg1	process					1347:1353	endothermic, entropy-driven, and thermodynamically spontaneous process	1284:1353	endothermic, entropy-driven, and thermodynamically spontaneous process	1284:1353	The adsorption process was found to be endothermic, entropy-driven, and thermodynamically spontaneous process.					
35104515	3	48	theme	separation	443:452	arg1	advantages					415:424	the advantages	411:424	the advantages	411:424	Nano-Fe3O4 has the advantages of easy magnetic separation and surface functionalization, which can improve the efficiency as well as selectivity of separation.					
35104515	6	49	theme	Fe3O4	754:758	arg1	nanosphere					771:780	MXene@Fe3O4@CSmagnetic nanosphere	748:780	MXene@Fe3O4@CSmagnetic nanosphere	748:780	In this study, MXene@Fe3O4@CSmagnetic nanosphere was synthesized by ultrasonic self-assembly to remove Congo red (CR).					
35104515	5	50	theme	CS	718:719	arg1	composites					721:730	new MXene@Fe3O4@CS composites	702:730	new MXene@Fe3O4@CS composites	702:730	MXene can provide suitable support for both CS and Fe3O4 to construct new MXene@Fe3O4@CS composites.					
35104515	10	51	theme	entropy-driven	1297:1310	arg1	process					1260:1266	The adsorption process	1245:1266	The adsorption process	1245:1266	The adsorption process was found to be endothermic, entropy-driven, and thermodynamically spontaneous process.					
35104515	10	51	theme	entropy-driven	1297:1310	arg1	process					1347:1353	endothermic, entropy-driven, and thermodynamically spontaneous process	1284:1353	endothermic, entropy-driven, and thermodynamically spontaneous process	1284:1353	The adsorption process was found to be endothermic, entropy-driven, and thermodynamically spontaneous process.					
35104515	5	52	theme	suitable	650:657	arg1	support					659:665	suitable support	650:665	suitable support for both CS and Fe3O4 to construct new MXene@Fe3O4@CS composites	650:730	MXene can provide suitable support for both CS and Fe3O4 to construct new MXene@Fe3O4@CS composites.					
35104515	6	53	theme	@	753:753	arg1	nanosphere					771:780	MXene@Fe3O4@CSmagnetic nanosphere	748:780	MXene@Fe3O4@CSmagnetic nanosphere	748:780	In this study, MXene@Fe3O4@CSmagnetic nanosphere was synthesized by ultrasonic self-assembly to remove Congo red (CR).					
35104515	7	54	theme	transmission	888:899	arg1	TEM					922:924	TEM	922:924	TEM	922:924	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	7	54	theme	transmission	888:899	arg1	microscopy					910:919	transmission electron microscopy	888:919	transmission electron microscopy (TEM)	888:925	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	3	55	theme	easy	429:432	arg1	separation					443:452	easy magnetic separation	429:452	easy magnetic separation	429:452	Nano-Fe3O4 has the advantages of easy magnetic separation and surface functionalization, which can improve the efficiency as well as selectivity of separation.					
35104515	0	56	theme	CS	65:66	arg1	nanospheres					68:78	MXene-supported Fe3O4@CS nanospheres	43:78	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.	0:134	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.					
35104515	6	57	theme	MXene	748:752	arg1	nanosphere					771:780	MXene@Fe3O4@CSmagnetic nanosphere	748:780	MXene@Fe3O4@CSmagnetic nanosphere	748:780	In this study, MXene@Fe3O4@CSmagnetic nanosphere was synthesized by ultrasonic self-assembly to remove Congo red (CR).					
35104515	7	58	theme	electron	901:908	arg1	TEM					922:924	TEM	922:924	TEM	922:924	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	7	58	theme	electron	901:908	arg1	microscopy					910:919	transmission electron microscopy	888:919	transmission electron microscopy (TEM)	888:925	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
35104515	3	59	theme	functionalization	466:482	arg1	advantages					415:424	the advantages	411:424	the advantages	411:424	Nano-Fe3O4 has the advantages of easy magnetic separation and surface functionalization, which can improve the efficiency as well as selectivity of separation.					
35104515	0	60	theme	@	64:64	arg1	nanospheres					68:78	MXene-supported Fe3O4@CS nanospheres	43:78	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.	0:134	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.					
35104515	0	61	dep	Application	0:10	arg1	nanospheres					68:78	MXene-supported Fe3O4@CS nanospheres	43:78	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.	0:134	Application of 3D magnetic nanocomposites: MXene-supported Fe3O4@CS nanospheres for highly efficient adsorption and separation of dyes.					
35104515	7	62	dep	Fourier	972:978	arg1	Transform					980:988	Transform	980:988	Transform Infrared (FT-IR) spectroscopy	980:1018	Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier Transform Infrared (FT-IR) spectroscopy were employed to characterize the nanocomposites.					
36835068	7	0	theme	low	1140:1142	arg1	weight					1165:1170	low and medium molecular weight	1140:1170	weight	1165:1170	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	9	1	theme	HA	1633:1634	arg1	mixtures					1650:1657	HA and secretome mixtures	1633:1657	HA and secretome mixtures	1633:1657	Moreover, an anti-inflammatory profile has been revealed when dealing with HA and secretome mixtures.					
36835068	10	2	from	accomplishment	1730:1743	arg1	diseases					1795:1802	respiratory diseases	1783:1802	respiratory diseases	1783:1802	Therefore, these promising results can allow important advance in the accomplishment of the future therapeutic approach in respiratory diseases, up to date still missing.					
36835068	7	3	theme	weight	1165:1170	arg1	HA					1136:1137	HA	1136:1137	HA (low and medium molecular weight HA)	1136:1174	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	7	3	theme	weight	1165:1170	arg1	HA					1172:1173	low and medium molecular weight HA	1140:1173	low and medium molecular weight HA	1140:1173	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	8	4	theme	tissue	1543:1548	arg1	repair					1550:1555	lung tissue repair	1538:1555	lung tissue repair	1538:1555	Likewise, cell viability and cell rate of migration were reported to be improved for HA and secretome blends, indicating an interesting potentiality of such systems for lung tissue repair.					
36835068	8	5	theme	interesting	1493:1503	arg1	potentiality					1505:1516	an interesting potentiality	1490:1516	an interesting potentiality of such systems for lung tissue repair	1490:1555	Likewise, cell viability and cell rate of migration were reported to be improved for HA and secretome blends, indicating an interesting potentiality of such systems for lung tissue repair.					
36835068	7	6	theme	secretome	1308:1316	arg1	solutions					1318:1326	secretome solutions	1308:1326	secretome solutions alone	1308:1332	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	10	7	theme	respiratory	1783:1793	arg1	diseases					1795:1802	respiratory diseases	1783:1802	respiratory diseases	1783:1802	Therefore, these promising results can allow important advance in the accomplishment of the future therapeutic approach in respiratory diseases, up to date still missing.					
36835068	4	8	theme	endothelial	661:671	arg1	permeability					688:699	endothelial and epithelial permeability	661:699	permeability	688:699	However, MSCs have the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth.					
36835068	5	9	theme	Alveolar	913:920	arg1	type					922:925	Alveolar type II	913:928	Alveolar type II (ATII) cells	913:941	Furthermore, Hyaluronic acid (HA) has been demonstrated to have particularly efficacy in promoting the differentiation of MSCs in Alveolar type II (ATII) cells.					
36835068	5	9	theme	Alveolar	913:920	arg1	ATII					931:934	ATII	931:934	ATII	931:934	Furthermore, Hyaluronic acid (HA) has been demonstrated to have particularly efficacy in promoting the differentiation of MSCs in Alveolar type II (ATII) cells.					
36835068	3	10	theme	Mesenchymal	357:367	arg1	MSCs					381:384	MSCs	381:384	MSCs	381:384	Cell-therapy based on Mesenchymal Stem Cells (MSCs) is an attractive, as well as new therapeutic approach, although some limitations can be ascribed for therapeutic use, such as tumorigenicity and immune rejection.					
36835068	3	10	theme	Mesenchymal	357:367	arg1	Cells					374:378	Mesenchymal Stem Cells	357:378	Mesenchymal Stem Cells (MSCs)	357:385	Cell-therapy based on Mesenchymal Stem Cells (MSCs) is an attractive, as well as new therapeutic approach, although some limitations can be ascribed for therapeutic use, such as tumorigenicity and immune rejection.					
36835068	7	11	theme	ng/mL	1274:1278	arg1	expression					1252:1261	SPC marker expression	1241:1261	SPC marker expression of about 5 ng/mL	1241:1278	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	5	12	contain	have	842:845	arg2	efficacy					860:867	efficacy	860:867	particularly efficacy	847:867	Furthermore, Hyaluronic acid (HA) has been demonstrated to have particularly efficacy in promoting the differentiation of MSCs in Alveolar type II (ATII) cells.					
36835068	5	12	contain	have	842:845	arg1	HA					813:814	HA	813:814	HA	813:814	Furthermore, Hyaluronic acid (HA) has been demonstrated to have particularly efficacy in promoting the differentiation of MSCs in Alveolar type II (ATII) cells.					
36835068	5	12	contain	have	842:845	arg1	acid					807:810	Hyaluronic acid	796:810	Hyaluronic acid (HA)	796:815	Furthermore, Hyaluronic acid (HA) has been demonstrated to have particularly efficacy in promoting the differentiation of MSCs in Alveolar type II (ATII) cells.					
36835068	2	13	theme	able	295:298	arg1	options					287:293	effective treatment options	267:293	effective treatment options able to restore the lung tissue damage	267:332	However, they have not yet been translated to effective treatment options able to restore the lung tissue damage.					
36835068	3	14	theme	immune	532:537	arg1	rejection					539:547	immune rejection	532:547	immune rejection	532:547	Cell-therapy based on Mesenchymal Stem Cells (MSCs) is an attractive, as well as new therapeutic approach, although some limitations can be ascribed for therapeutic use, such as tumorigenicity and immune rejection.					
36835068	3	15	theme	Stem	369:372	arg1	MSCs					381:384	MSCs	381:384	MSCs	381:384	Cell-therapy based on Mesenchymal Stem Cells (MSCs) is an attractive, as well as new therapeutic approach, although some limitations can be ascribed for therapeutic use, such as tumorigenicity and immune rejection.					
36835068	3	15	theme	Stem	369:372	arg1	Cells					374:378	Mesenchymal Stem Cells	357:378	Mesenchymal Stem Cells (MSCs)	357:385	Cell-therapy based on Mesenchymal Stem Cells (MSCs) is an attractive, as well as new therapeutic approach, although some limitations can be ascribed for therapeutic use, such as tumorigenicity and immune rejection.					
36835068	10	16	theme	promising	1677:1685	arg1	results					1687:1693	these promising results	1671:1693	these promising results	1671:1693	Therefore, these promising results can allow important advance in the accomplishment of the future therapeutic approach in respiratory diseases, up to date still missing.					
36835068	7	17	theme	SPC	1335:1337	arg1	ng/mL					1347:1351	SPC about 3 ng/mL	1335:1351	SPC about 3 ng/mL	1335:1351	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	7	18	theme	ATII	1229:1232	arg1	cells					1234:1238	ATII cells	1229:1238	ATII cells	1229:1238	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	10	19	from	approach	1771:1778	arg1	diseases					1795:1802	respiratory diseases	1783:1802	respiratory diseases	1783:1802	Therefore, these promising results can allow important advance in the accomplishment of the future therapeutic approach in respiratory diseases, up to date still missing.					
36835068	8	20	theme	cell	1398:1401	arg1	rate					1403:1406	cell rate	1398:1406	cell rate	1398:1406	Likewise, cell viability and cell rate of migration were reported to be improved for HA and secretome blends, indicating an interesting potentiality of such systems for lung tissue repair.					
36835068	9	21	theme	secretome	1640:1648	arg1	mixtures					1650:1657	HA and secretome mixtures	1633:1657	HA and secretome mixtures	1633:1657	Moreover, an anti-inflammatory profile has been revealed when dealing with HA and secretome mixtures.					
36835068	4	22	theme	multiple	593:600	arg1	factors					612:618	multiple paracrine factors	593:618	multiple paracrine factors	593:618	However, MSCs have the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth.					
36835068	4	22	theme	multiple	593:600	arg1	secretome					628:636	secretome	628:636	secretome	628:636	However, MSCs have the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth.					
36835068	2	23	theme	treatment	277:285	arg1	options					287:293	effective treatment options	267:293	effective treatment options able to restore the lung tissue damage	267:332	However, they have not yet been translated to effective treatment options able to restore the lung tissue damage.					
36835068	6	24	theme	lung	1010:1013	arg1	regeneration					1022:1033	the lung tissue regeneration	1006:1033	the lung tissue regeneration	1006:1033	In this frame, the combination of HA and secretome to achieve the lung tissue regeneration has been investigated for the first time in this work.					
36835068	7	25	theme	marker	1245:1250	arg1	expression					1252:1261	SPC marker expression	1241:1261	SPC marker expression of about 5 ng/mL	1241:1278	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	7	26	theme	about	1339:1343	arg1	ng/mL					1347:1351	SPC about 3 ng/mL	1335:1351	SPC about 3 ng/mL	1335:1351	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	6	27	theme	first	1065:1069	arg1	time					1071:1074	the first time	1061:1074	the first time in this work	1061:1087	In this frame, the combination of HA and secretome to achieve the lung tissue regeneration has been investigated for the first time in this work.					
36835068	10	28	from	diseases	1795:1802	arg1	accomplishment					1730:1743	the accomplishment	1726:1743	the accomplishment of the future therapeutic approach in respiratory diseases	1726:1802	Therefore, these promising results can allow important advance in the accomplishment of the future therapeutic approach in respiratory diseases, up to date still missing.					
36835068	2	29	theme	effective	267:275	arg1	options					287:293	effective treatment options	267:293	effective treatment options able to restore the lung tissue damage	267:332	However, they have not yet been translated to effective treatment options able to restore the lung tissue damage.					
36835068	3	30	theme	new	416:418	arg1	approach					432:439	new therapeutic approach	416:439	new therapeutic approach	416:439	Cell-therapy based on Mesenchymal Stem Cells (MSCs) is an attractive, as well as new therapeutic approach, although some limitations can be ascribed for therapeutic use, such as tumorigenicity and immune rejection.					
36835068	10	31	theme	missing	1822:1828	arg1	date					1811:1814	date	1811:1814	date still missing	1811:1828	Therefore, these promising results can allow important advance in the accomplishment of the future therapeutic approach in respiratory diseases, up to date still missing.					
36835068	2	32	theme	lung	315:318	arg1	damage					327:332	the lung tissue damage	311:332	the lung tissue damage	311:332	However, they have not yet been translated to effective treatment options able to restore the lung tissue damage.					
36835068	4	33	theme	capable	639:645	arg1	factors					612:618	multiple paracrine factors	593:618	multiple paracrine factors	593:618	However, MSCs have the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth.					
36835068	4	33	theme	capable	639:645	arg1	secretome					628:636	secretome	628:636	secretome	628:636	However, MSCs have the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth.					
36835068	5	34	theme	Hyaluronic	796:805	arg1	HA					813:814	HA	813:814	HA	813:814	Furthermore, Hyaluronic acid (HA) has been demonstrated to have particularly efficacy in promoting the differentiation of MSCs in Alveolar type II (ATII) cells.					
36835068	5	34	theme	Hyaluronic	796:805	arg1	acid					807:810	Hyaluronic acid	796:810	Hyaluronic acid (HA)	796:815	Furthermore, Hyaluronic acid (HA) has been demonstrated to have particularly efficacy in promoting the differentiation of MSCs in Alveolar type II (ATII) cells.					
36835068	1	35	from	therapies	127:135	arg1	diseases					145:152	lung diseases	140:152	lung diseases	140:152	Pharmacological therapies in lung diseases are nowadays useful in reducing the symptomatology of lung injury.					
36835068	5	36	theme	type	922:925	arg1	cells					937:941	Alveolar type II (ATII) cells	913:941	Alveolar type II (ATII) cells	913:941	Furthermore, Hyaluronic acid (HA) has been demonstrated to have particularly efficacy in promoting the differentiation of MSCs in Alveolar type II (ATII) cells.					
36835068	7	37	theme	SPC	1241:1243	arg1	expression					1252:1261	SPC marker expression	1241:1261	SPC marker expression of about 5 ng/mL	1241:1278	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	0	38	theme	Acid	21:24	arg1	Effect					0:5	Effect	0:5	Effect of Hyaluronic Acid and Mesenchymal Stem Cells	0:51	Effect of Hyaluronic Acid and Mesenchymal Stem Cells Secretome Combination in Promoting Alveolar Regeneration.					
36835068	5	39	from	differentiation	886:900	arg1	cells					937:941	Alveolar type II (ATII) cells	913:941	Alveolar type II (ATII) cells	913:941	Furthermore, Hyaluronic acid (HA) has been demonstrated to have particularly efficacy in promoting the differentiation of MSCs in Alveolar type II (ATII) cells.					
36835068	4	40	theme	tissue	735:740	arg1	repair					742:747	tissue repair	735:747	tissue repair	735:747	However, MSCs have the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth.					
36835068	1	41	theme	lung	208:211	arg1	injury					213:218	lung injury	208:218	lung injury	208:218	Pharmacological therapies in lung diseases are nowadays useful in reducing the symptomatology of lung injury.					
36835068	0	42	theme	Hyaluronic	10:19	arg1	Acid					21:24	Hyaluronic Acid	10:24	Hyaluronic Acid	10:24	Effect of Hyaluronic Acid and Mesenchymal Stem Cells Secretome Combination in Promoting Alveolar Regeneration.					
36835068	7	43	dep	enhance	1197:1203	arg1	expression					1252:1261	SPC marker expression	1241:1261	SPC marker expression of about 5 ng/mL	1241:1278	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	1	44	theme	Pharmacological	111:125	arg1	therapies					127:135	Pharmacological therapies	111:135	Pharmacological therapies in lung diseases	111:152	Pharmacological therapies in lung diseases are nowadays useful in reducing the symptomatology of lung injury.					
36835068	1	45	theme	injury	213:218	arg1	symptomatology					190:203	the symptomatology	186:203	the symptomatology of lung injury	186:218	Pharmacological therapies in lung diseases are nowadays useful in reducing the symptomatology of lung injury.					
36835068	0	46	theme	Mesenchymal	30:40	arg1	Cells					47:51	Mesenchymal Stem Cells	30:51	Mesenchymal Stem Cells	30:51	Effect of Hyaluronic Acid and Mesenchymal Stem Cells Secretome Combination in Promoting Alveolar Regeneration.					
36835068	7	47	theme	MSCs	1205:1208	arg1	differentiation					1210:1224	MSCs differentiation	1205:1224	MSCs differentiation	1205:1224	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	9	48	theme	anti-inflammatory	1571:1587	arg1	profile					1589:1595	an anti-inflammatory profile	1568:1595	an anti-inflammatory profile	1568:1595	Moreover, an anti-inflammatory profile has been revealed when dealing with HA and secretome mixtures.					
36835068	6	49	theme	secretome	985:993	arg1	combination					963:973	the combination	959:973	the combination of HA and secretome to achieve the lung tissue regeneration	959:1033	In this frame, the combination of HA and secretome to achieve the lung tissue regeneration has been investigated for the first time in this work.					
36835068	10	50	dep	important	1705:1713	arg1	advance					1715:1721	advance	1715:1721	advance in the accomplishment of the future therapeutic approach in respiratory diseases	1715:1802	Therefore, these promising results can allow important advance in the accomplishment of the future therapeutic approach in respiratory diseases, up to date still missing.					
36835068	3	51	theme	therapeutic	420:430	arg1	approach					432:439	new therapeutic approach	416:439	new therapeutic approach	416:439	Cell-therapy based on Mesenchymal Stem Cells (MSCs) is an attractive, as well as new therapeutic approach, although some limitations can be ascribed for therapeutic use, such as tumorigenicity and immune rejection.					
36835068	7	52	theme	Overall	1090:1096	arg1	results					1098:1104	Overall results	1090:1104	Overall results	1090:1104	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	4	53	contain	have	564:567	arg1	MSCs					559:562	MSCs	559:562	MSCs	559:562	However, MSCs have the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth.					
36835068	4	53	contain	have	564:567	arg2	capacity					573:580	the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth	569:780	the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth	569:780	However, MSCs have the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth.					
36835068	0	54	theme	Cells	47:51	arg1	Effect					0:5	Effect	0:5	Effect of Hyaluronic Acid and Mesenchymal Stem Cells	0:51	Effect of Hyaluronic Acid and Mesenchymal Stem Cells Secretome Combination in Promoting Alveolar Regeneration.					
36835068	7	55	theme	HA	1136:1137	arg1	combination					1121:1131	the combination	1117:1131	the combination of HA (low and medium molecular weight HA) plus secretome	1117:1189	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	8	56	theme	migration	1411:1419	arg1	viability					1384:1392	cell viability	1379:1392	cell viability	1379:1392	Likewise, cell viability and cell rate of migration were reported to be improved for HA and secretome blends, indicating an interesting potentiality of such systems for lung tissue repair.					
36835068	8	56	theme	migration	1411:1419	arg1	rate					1403:1406	cell rate	1398:1406	cell rate	1398:1406	Likewise, cell viability and cell rate of migration were reported to be improved for HA and secretome blends, indicating an interesting potentiality of such systems for lung tissue repair.					
36835068	0	57	theme	Stem	42:45	arg1	Cells					47:51	Mesenchymal Stem Cells	30:51	Mesenchymal Stem Cells	30:51	Effect of Hyaluronic Acid and Mesenchymal Stem Cells Secretome Combination in Promoting Alveolar Regeneration.					
36835068	10	58	theme	approach	1771:1778	arg1	accomplishment					1730:1743	the accomplishment	1726:1743	the accomplishment of the future therapeutic approach in respiratory diseases	1726:1802	Therefore, these promising results can allow important advance in the accomplishment of the future therapeutic approach in respiratory diseases, up to date still missing.					
36835068	4	59	theme	epithelial	677:686	arg1	permeability					688:699	endothelial and epithelial permeability	661:699	permeability	688:699	However, MSCs have the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth.					
36835068	8	60	theme	systems	1526:1532	arg1	potentiality					1505:1516	an interesting potentiality	1490:1516	an interesting potentiality of such systems for lung tissue repair	1490:1555	Likewise, cell viability and cell rate of migration were reported to be improved for HA and secretome blends, indicating an interesting potentiality of such systems for lung tissue repair.					
36835068	8	61	theme	such	1521:1524	arg1	systems					1526:1532	such systems	1521:1532	such systems	1521:1532	Likewise, cell viability and cell rate of migration were reported to be improved for HA and secretome blends, indicating an interesting potentiality of such systems for lung tissue repair.					
36835068	10	62	theme	therapeutic	1759:1769	arg1	approach					1771:1778	the future therapeutic approach	1748:1778	the future therapeutic approach in respiratory diseases	1748:1802	Therefore, these promising results can allow important advance in the accomplishment of the future therapeutic approach in respiratory diseases, up to date still missing.					
36835068	6	63	from	time	1071:1074	arg1	work					1084:1087	this work	1079:1087	this work	1079:1087	In this frame, the combination of HA and secretome to achieve the lung tissue regeneration has been investigated for the first time in this work.					
36835068	1	64	theme	lung	140:143	arg1	diseases					145:152	lung diseases	140:152	lung diseases	140:152	Pharmacological therapies in lung diseases are nowadays useful in reducing the symptomatology of lung injury.					
36835068	7	65	theme	only	1297:1300	arg1	HA					1302:1303	only HA	1297:1303	only HA	1297:1303	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	6	66	theme	HA	978:979	arg1	combination					963:973	the combination	959:973	the combination of HA and secretome to achieve the lung tissue regeneration	959:1033	In this frame, the combination of HA and secretome to achieve the lung tissue regeneration has been investigated for the first time in this work.					
36835068	10	67	theme	future	1752:1757	arg1	approach					1771:1778	the future therapeutic approach	1748:1778	the future therapeutic approach in respiratory diseases	1748:1802	Therefore, these promising results can allow important advance in the accomplishment of the future therapeutic approach in respiratory diseases, up to date still missing.					
36835068	4	68	theme	bacterial	765:773	arg1	growth					775:780	bacterial growth	765:780	bacterial growth	765:780	However, MSCs have the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth.					
36835068	2	69	theme	tissue	320:325	arg1	damage					327:332	the lung tissue damage	311:332	the lung tissue damage	311:332	However, they have not yet been translated to effective treatment options able to restore the lung tissue damage.					
36835068	8	70	theme	lung	1538:1541	arg1	repair					1550:1555	lung tissue repair	1538:1555	lung tissue repair	1538:1555	Likewise, cell viability and cell rate of migration were reported to be improved for HA and secretome blends, indicating an interesting potentiality of such systems for lung tissue repair.					
36835068	5	71	theme	MSCs	905:908	arg1	differentiation					886:900	the differentiation	882:900	the differentiation of MSCs in Alveolar type II (ATII) cells	882:941	Furthermore, Hyaluronic acid (HA) has been demonstrated to have particularly efficacy in promoting the differentiation of MSCs in Alveolar type II (ATII) cells.					
36835068	7	72	theme	medium	1148:1153	arg1	weight					1165:1170	low and medium molecular weight	1140:1170	weight	1165:1170	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	4	73	theme	paracrine	602:610	arg1	factors					612:618	multiple paracrine factors	593:618	multiple paracrine factors	593:618	However, MSCs have the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth.					
36835068	4	73	theme	paracrine	602:610	arg1	secretome					628:636	secretome	628:636	secretome	628:636	However, MSCs have the capacity to secrete multiple paracrine factors, namely secretome, capable of regulating endothelial and epithelial permeability, decrease inflammation, enhancing tissue repair, and inhibiting bacterial growth.					
36835068	3	74	theme	therapeutic	488:498	arg1	use					500:502	therapeutic use	488:502	therapeutic use	488:502	Cell-therapy based on Mesenchymal Stem Cells (MSCs) is an attractive, as well as new therapeutic approach, although some limitations can be ascribed for therapeutic use, such as tumorigenicity and immune rejection.					
36835068	6	75	theme	tissue	1015:1020	arg1	regeneration					1022:1033	the lung tissue regeneration	1006:1033	the lung tissue regeneration	1006:1033	In this frame, the combination of HA and secretome to achieve the lung tissue regeneration has been investigated for the first time in this work.					
36835068	7	76	theme	molecular	1155:1163	arg1	weight					1165:1170	low and medium molecular weight	1140:1170	weight	1165:1170	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	8	77	theme	cell	1379:1382	arg1	viability					1384:1392	cell viability	1379:1392	cell viability	1379:1392	Likewise, cell viability and cell rate of migration were reported to be improved for HA and secretome blends, indicating an interesting potentiality of such systems for lung tissue repair.					
36835068	8	78	theme	secretome	1461:1469	arg1	blends					1471:1476	secretome blends	1461:1476	secretome blends	1461:1476	Likewise, cell viability and cell rate of migration were reported to be improved for HA and secretome blends, indicating an interesting potentiality of such systems for lung tissue repair.					
36835068	7	79	theme	secretome	1181:1189	arg1	combination					1121:1131	the combination	1117:1131	the combination of HA (low and medium molecular weight HA) plus secretome	1117:1189	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	7	80	dep	HA	1302:1303	arg1	ng/mL					1347:1351	SPC about 3 ng/mL	1335:1351	SPC about 3 ng/mL	1335:1351	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	7	80	dep	HA	1302:1303	arg1	the					1293:1295	the	1293:1295	the	1293:1295	Overall results showed how the combination of HA (low and medium molecular weight HA) plus secretome could enhance MSCs differentiation in ATII cells (SPC marker expression of about 5 ng/mL) compared to the only HA or secretome solutions alone (SPC about 3 ng/mL, respectively).					
36835068	0	81	theme	Alveolar	88:95	arg1	Regeneration					97:108	Alveolar Regeneration	88:108	Alveolar Regeneration	88:108	Effect of Hyaluronic Acid and Mesenchymal Stem Cells Secretome Combination in Promoting Alveolar Regeneration.					
36976224	0	0	theme	Chitosan-Derived	63:78	arg1	Scaffold					102:109	a Chitosan-Derived Epigenetic Modulation Scaffold	61:109	a Chitosan-Derived Epigenetic Modulation Scaffold	61:109	Effect of Storage Time and Temperature on the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold.					
36976224	7	1	theme	CS/BCP/TSA	1139:1148	arg1	scaffold					1150:1157	the CS/BCP/TSA scaffold	1135:1157	the CS/BCP/TSA scaffold	1135:1157	Thus, the CS/BCP/TSA scaffold should be stored in freezing conditions to preserve the long-term stability of TSA.					
36976224	3	2	theme	appropriate	516:526	arg1	temperature					536:546	appropriate storage temperature	516:546	appropriate storage temperature	516:546	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	5	3	theme	Different	823:831	arg1	times					841:845	Different storage times	823:845	Different storage times (0, 14, and 28 days)	823:866	Different storage times (0, 14, and 28 days) and temperatures (-18, 4, and 25 °C) did not affect the porosity, compressive strength, shape memory, and amount of TSA released.					
36976224	5	3	theme	Different	823:831	arg1	days					862:865	0, 14, and 28 days	848:865	0, 14, and 28 days	848:865	Different storage times (0, 14, and 28 days) and temperatures (-18, 4, and 25 °C) did not affect the porosity, compressive strength, shape memory, and amount of TSA released.					
36976224	3	4	theme	storage	528:534	arg1	temperature					536:546	appropriate storage temperature	516:546	appropriate storage temperature	516:546	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	3	5	dep	Phosphate/Trichostatin	577:598	arg1	scaffold					612:619	A composite scaffold	600:619	Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold)	551:641	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	3	5	dep	Phosphate/Trichostatin	577:598	arg1	scaffold					633:640	CS/BCP/TSA scaffold	622:640	CS/BCP/TSA scaffold	622:640	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	0	6	theme	Modulation	91:100	arg1	Scaffold					102:109	a Chitosan-Derived Epigenetic Modulation Scaffold	61:109	a Chitosan-Derived Epigenetic Modulation Scaffold	61:109	Effect of Storage Time and Temperature on the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold.					
36976224	0	7	from	Effect	0:5	arg1	Bioactivity					46:56	the Bioactivity	42:56	the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold	42:109	Effect of Storage Time and Temperature on the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold.					
36976224	6	8	theme	storage	1098:1104	arg1	periods					1106:1112	7-day storage periods	1092:1112	7-day storage periods	1092:1112	However, scaffolds stored at 25 °C and 4 °C were found to lose their bioactivity after 3- and 7-day storage periods, respectively.					
36976224	2	9	theme	composite	310:318	arg1	scaffold					320:327	a chitosan-derived composite scaffold	291:327	a chitosan-derived composite scaffold incorporated with bioactive molecules	291:365	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	3	10	dep	time	507:510	arg1	the					495:497	the	495:497	the	495:497	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	5	11	theme	compressive	934:944	arg1	strength					946:953	compressive strength	934:953	compressive strength	934:953	Different storage times (0, 14, and 28 days) and temperatures (-18, 4, and 25 °C) did not affect the porosity, compressive strength, shape memory, and amount of TSA released.					
36976224	0	12	theme	Epigenetic	80:89	arg1	Scaffold					102:109	a Chitosan-Derived Epigenetic Modulation Scaffold	61:109	a Chitosan-Derived Epigenetic Modulation Scaffold	61:109	Effect of Storage Time and Temperature on the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold.					
36976224	2	13	theme	chitosan-derived	293:308	arg1	scaffold					320:327	a chitosan-derived composite scaffold	291:327	a chitosan-derived composite scaffold incorporated with bioactive molecules	291:365	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	2	14	dep	material	401:408	arg1	repair					413:418	repair	413:418	repair	413:418	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	1	15	theme	main	159:162	arg1	limitations					164:174	the main limitations	155:174	the main limitations of translating tissue engineering technology to commercialized clinical applications	155:259	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	0	16	theme	Scaffold	102:109	arg1	Bioactivity					46:56	the Bioactivity	42:56	the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold	42:109	Effect of Storage Time and Temperature on the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold.					
36976224	4	17	attach	released	728:735	arg1	scaffolds					753:761	CS/BCP/TSA scaffolds	742:761	CS/BCP/TSA scaffolds in different storage times and temperatures	742:805	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	4	17	attach	released	728:735	arg2	A					720:720	trichostatin A	707:720	trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures	707:805	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	4	17	attach	released	728:735	arg2	TSA					723:725	TSA	723:725	TSA	723:725	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	5	18	theme	storage	833:839	arg1	times					841:845	Different storage times	823:845	Different storage times (0, 14, and 28 days)	823:866	Different storage times (0, 14, and 28 days) and temperatures (-18, 4, and 25 °C) did not affect the porosity, compressive strength, shape memory, and amount of TSA released.					
36976224	5	18	theme	storage	833:839	arg1	days					862:865	0, 14, and 28 days	848:865	0, 14, and 28 days	848:865	Different storage times (0, 14, and 28 days) and temperatures (-18, 4, and 25 °C) did not affect the porosity, compressive strength, shape memory, and amount of TSA released.					
36976224	3	19	theme	Calcium	569:575	arg1	Phosphate/Trichostatin					577:598	Chitosan/Biphasic Calcium Phosphate/Trichostatin	551:598	Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold)	551:641	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	1	20	theme	limitations	164:174	arg1	one					148:150	one	148:150	one	148:150	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	1	20	theme	limitations	164:174	arg1	protocol					136:143	The appropriate storage protocol	112:143	The appropriate storage protocol	112:143	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	1	20	theme	limitations	164:174	arg1	limitations					164:174	the main limitations	155:174	the main limitations of translating tissue engineering technology to commercialized clinical applications	155:259	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	6	21	theme	7-day	1092:1096	arg1	periods					1106:1112	7-day storage periods	1092:1112	7-day storage periods	1092:1112	However, scaffolds stored at 25 °C and 4 °C were found to lose their bioactivity after 3- and 7-day storage periods, respectively.					
36976224	2	22	theme	bioactive	347:355	arg1	molecules					357:365	bioactive molecules	347:365	bioactive molecules	347:365	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	4	23	theme	trichostatin	707:718	arg1	TSA					723:725	TSA	723:725	TSA	723:725	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	4	23	theme	trichostatin	707:718	arg1	A					720:720	trichostatin A	707:720	trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures	707:805	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	3	24	theme	CS/BCP/TSA	622:631	arg1	scaffold					612:619	A composite scaffold	600:619	Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold)	551:641	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	3	24	theme	CS/BCP/TSA	622:631	arg1	scaffold					633:640	CS/BCP/TSA scaffold	622:640	CS/BCP/TSA scaffold	622:640	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	4	25	theme	A	720:720	arg1	bioactivity					692:702	in vitro bioactivity	683:702	in vitro bioactivity	683:702	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	4	25	theme	A	720:720	arg1	properties					668:677	The mechanical properties	653:677	The mechanical properties	653:677	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	0	26	theme	Time	18:21	arg1	Effect					0:5	Effect	0:5	Effect of Storage Time and Temperature on the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold.	0:110	Effect of Storage Time and Temperature on the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold.					
36976224	4	27	theme	storage	776:782	arg1	times					784:788	different storage times	766:788	different storage times	766:788	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	3	28	theme	composite	602:610	arg1	scaffold					612:619	A composite scaffold	600:619	Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold)	551:641	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	3	28	theme	composite	602:610	arg1	scaffold					633:640	CS/BCP/TSA scaffold	622:640	CS/BCP/TSA scaffold	622:640	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	1	29	theme	tissue	191:196	arg1	technology					210:219	tissue engineering technology	191:219	tissue engineering technology	191:219	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	2	30	theme	mice	451:454	arg1	calvaria					456:463	mice calvaria	451:463	mice calvaria	451:463	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	3	31	theme	Chitosan/Biphasic	551:567	arg1	Phosphate/Trichostatin					577:598	Chitosan/Biphasic Calcium Phosphate/Trichostatin	551:598	Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold)	551:641	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	2	32	theme	scaffold	320:327	arg1	material					401:408	an excellent material	388:408	an excellent material to repair a critical size bony defect in mice calvaria	388:463	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	2	32	theme	scaffold	320:327	arg1	development					276:286	the development	272:286	the development of a chitosan-derived composite scaffold incorporated with bioactive molecules	272:365	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	1	33	theme	engineering	198:208	arg1	technology					210:219	tissue engineering technology	191:219	tissue engineering technology	191:219	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	0	34	theme	Temperature	27:37	arg1	Effect					0:5	Effect	0:5	Effect of Storage Time and Temperature on the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold.	0:110	Effect of Storage Time and Temperature on the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold.					
36976224	2	35	link	chitosan-derived	293:308	arg1	scaffold					320:327	a chitosan-derived composite scaffold	291:327	a chitosan-derived composite scaffold incorporated with bioactive molecules	291:365	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	2	36	theme	bony	436:439	arg1	defect					441:446	a critical size bony defect	420:446	a critical size bony defect	420:446	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	2	37	theme	critical	422:429	arg1	defect					441:446	a critical size bony defect	420:446	a critical size bony defect	420:446	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	5	38	theme	TSA	984:986	arg1	porosity					924:931	the porosity	920:931	the porosity	920:931	Different storage times (0, 14, and 28 days) and temperatures (-18, 4, and 25 °C) did not affect the porosity, compressive strength, shape memory, and amount of TSA released.					
36976224	5	38	theme	TSA	984:986	arg1	strength					946:953	compressive strength	934:953	compressive strength	934:953	Different storage times (0, 14, and 28 days) and temperatures (-18, 4, and 25 °C) did not affect the porosity, compressive strength, shape memory, and amount of TSA released.					
36976224	5	38	theme	TSA	984:986	arg1	memory					962:967	shape memory	956:967	shape memory	956:967	Different storage times (0, 14, and 28 days) and temperatures (-18, 4, and 25 °C) did not affect the porosity, compressive strength, shape memory, and amount of TSA released.					
36976224	5	38	theme	TSA	984:986	arg1	amount					974:979	amount	974:979	amount	974:979	Different storage times (0, 14, and 28 days) and temperatures (-18, 4, and 25 °C) did not affect the porosity, compressive strength, shape memory, and amount of TSA released.					
36976224	5	38	theme	TSA	984:986	arg1	TSA					984:986	TSA	984:986	TSA released	984:995	Different storage times (0, 14, and 28 days) and temperatures (-18, 4, and 25 °C) did not affect the porosity, compressive strength, shape memory, and amount of TSA released.					
36976224	2	39	from	repair	413:418	arg1	calvaria					456:463	mice calvaria	451:463	mice calvaria	451:463	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	2	40	theme	size	431:434	arg1	defect					441:446	a critical size bony defect	420:446	a critical size bony defect	420:446	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	4	41	dep	in	683:684	arg1	vitro					686:690	vitro	686:690	vitro	686:690	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	4	42	theme	mechanical	657:666	arg1	properties					668:677	The mechanical properties	653:677	The mechanical properties	653:677	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	1	43	theme	appropriate	116:126	arg1	one					148:150	one	148:150	one	148:150	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	1	43	theme	appropriate	116:126	arg1	protocol					136:143	The appropriate storage protocol	112:143	The appropriate storage protocol	112:143	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	1	43	theme	appropriate	116:126	arg1	limitations					164:174	the main limitations	155:174	the main limitations of translating tissue engineering technology to commercialized clinical applications	155:259	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	4	44	theme	different	766:774	arg1	times					784:788	different storage times	766:788	different storage times	766:788	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	1	45	theme	storage	128:134	arg1	one					148:150	one	148:150	one	148:150	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	1	45	theme	storage	128:134	arg1	protocol					136:143	The appropriate storage protocol	112:143	The appropriate storage protocol	112:143	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	1	45	theme	storage	128:134	arg1	limitations					164:174	the main limitations	155:174	the main limitations of translating tissue engineering technology to commercialized clinical applications	155:259	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	1	46	theme	commercialized	224:237	arg1	applications					248:259	commercialized clinical applications	224:259	commercialized clinical applications	224:259	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	5	47	theme	shape	956:960	arg1	memory					962:967	shape memory	956:967	shape memory	956:967	Different storage times (0, 14, and 28 days) and temperatures (-18, 4, and 25 °C) did not affect the porosity, compressive strength, shape memory, and amount of TSA released.					
36976224	7	48	theme	long-term	1215:1223	arg1	stability					1225:1233	the long-term stability	1211:1233	the long-term stability of TSA	1211:1240	Thus, the CS/BCP/TSA scaffold should be stored in freezing conditions to preserve the long-term stability of TSA.					
36976224	3	49	theme	Phosphate/Trichostatin	577:598	arg1	time					507:510	storage time	499:510	storage time	499:510	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	3	49	theme	Phosphate/Trichostatin	577:598	arg1	temperature					536:546	appropriate storage temperature	516:546	appropriate storage temperature	516:546	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	3	50	theme	storage	499:505	arg1	time					507:510	storage time	499:510	storage time	499:510	This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro.					
36976224	4	51	from	scaffolds	753:761	arg1	temperatures					794:805	temperatures	794:805	temperatures	794:805	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	4	51	from	scaffolds	753:761	arg1	times					784:788	different storage times	766:788	different storage times	766:788	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	1	52	theme	clinical	239:246	arg1	applications					248:259	commercialized clinical applications	224:259	commercialized clinical applications	224:259	The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications.					
36976224	4	53	theme	in	683:684	arg1	bioactivity					692:702	in vitro bioactivity	683:702	in vitro bioactivity	683:702	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
36976224	7	54	theme	freezing	1179:1186	arg1	conditions					1188:1197	freezing conditions	1179:1197	freezing conditions	1179:1197	Thus, the CS/BCP/TSA scaffold should be stored in freezing conditions to preserve the long-term stability of TSA.					
36976224	7	55	theme	TSA	1238:1240	arg1	stability					1225:1233	the long-term stability	1211:1233	the long-term stability of TSA	1211:1240	Thus, the CS/BCP/TSA scaffold should be stored in freezing conditions to preserve the long-term stability of TSA.					
36976224	0	56	theme	Storage	10:16	arg1	Time					18:21	Storage Time	10:21	Storage Time	10:21	Effect of Storage Time and Temperature on the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold.					
36976224	2	57	theme	excellent	391:399	arg1	development					276:286	the development	272:286	the development of a chitosan-derived composite scaffold incorporated with bioactive molecules	272:365	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	2	57	theme	excellent	391:399	arg1	material					401:408	an excellent material	388:408	an excellent material to repair a critical size bony defect in mice calvaria	388:463	Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria.					
36976224	4	58	theme	CS/BCP/TSA	742:751	arg1	scaffolds					753:761	CS/BCP/TSA scaffolds	742:761	CS/BCP/TSA scaffolds in different storage times and temperatures	742:805	The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated.					
35026220	7	0	theme	minced	1311:1316	arg1	samples					1323:1329	the minced meat samples	1307:1329	the minced meat samples	1307:1329	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	0	1	theme	meat	160:163	arg1	properties					134:143	chemical and microbial properties	111:143	chemical and microbial properties of minced beef meat	111:163	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	4	2	theme	composite	894:902	arg1	films					904:908	the composite films	890:908	the composite films	890:908	The results showed that an increase in the level of the MPI led to a significant increment in TS and water-proof properties of the composite films.					
35026220	7	3	theme	chemical	1286:1293	arg1	analyses					1295:1302	the bacterial and chemical analyses	1268:1302	the bacterial and chemical analyses of the minced meat samples	1268:1329	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	7	4	theme	bacterial	1272:1280	arg1	analyses					1295:1302	the bacterial and chemical analyses	1268:1302	the bacterial and chemical analyses of the minced meat samples	1268:1329	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	3	5	theme	different	647:655	arg1	ratios					657:662	the different ratios	643:662	the different ratios of combination of MEO and MPI/PU	643:695	Also, depend on the different ratios of combination of MEO and MPI/PU, tensile strength (TS) and elongation at break (EAB) were varied.					
35026220	1	6	theme	minced	394:399	arg1	meat					406:409	minced beef meat	394:409	minced beef meat	394:409	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	6	7	theme	radical	1139:1145	arg1	scavenging					1147:1156	DPPH radical scavenging	1134:1156	DPPH radical scavenging	1134:1156	In addition, enrichment of the films with MEO led to a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli).					
35026220	4	8	from	increase	790:797	arg1	level					806:810	the level	802:810	the level of the MPI	802:821	The results showed that an increase in the level of the MPI led to a significant increment in TS and water-proof properties of the composite films.					
35026220	7	9	theme	antimicrobial	1445:1457	arg1	activity					1459:1466	effective antimicrobial activity	1435:1466	effective antimicrobial activity	1435:1466	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	6	10	theme	considerable	1102:1113	arg1	effect					1124:1129	a considerable positive effect	1100:1129	a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli)	1100:1256	In addition, enrichment of the films with MEO led to a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli).					
35026220	6	11	theme	positive	1115:1122	arg1	effect					1124:1129	a considerable positive effect	1100:1129	a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli)	1100:1256	In addition, enrichment of the films with MEO led to a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli).					
35026220	3	12	theme	at	735:736	arg1	EAB					745:747	EAB	745:747	EAB	745:747	Also, depend on the different ratios of combination of MEO and MPI/PU, tensile strength (TS) and elongation at break (EAB) were varied.					
35026220	3	12	theme	at	735:736	arg1	break					738:742	elongation at break	724:742	elongation at break (EAB)	724:748	Also, depend on the different ratios of combination of MEO and MPI/PU, tensile strength (TS) and elongation at break (EAB) were varied.					
35026220	0	13	theme	minced	148:153	arg1	meat					160:163	minced beef meat	148:163	minced beef meat	148:163	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	4	14	theme	MPI	819:821	arg1	level					806:810	the level	802:810	the level of the MPI	802:821	The results showed that an increase in the level of the MPI led to a significant increment in TS and water-proof properties of the composite films.					
35026220	0	15	theme	marjoram	62:69	arg1	Origanum					72:79	Origanum	72:79	Origanum	72:79	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	0	15	theme	marjoram	62:69	arg1	oil					104:106	marjoram (Origanum majorana L.) essential oil	62:106	marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat	62:163	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	7	16	theme	samples	1410:1416	arg1	stability					1385:1393	oxidative stability	1375:1393	oxidative stability of minced beef samples	1375:1416	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	6	17	theme	pathogenic	1193:1202	arg1	bacteria					1204:1211	pathogenic bacteria	1193:1211	pathogenic bacteria (Staphylococcus aureus and Escherichia coli)	1193:1256	In addition, enrichment of the films with MEO led to a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli).					
35026220	1	18	theme	mung	296:299	arg1	/pullulan					327:335	mung bean protein isolate (MPI)/pullulan	296:335	mung bean protein isolate (MPI)/pullulan	296:335	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	7	19	theme	minced	1398:1403	arg1	samples					1410:1416	minced beef samples	1398:1416	minced beef samples	1398:1416	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	1	20	dep	mechanical	231:240	arg1	composite					342:350	composite	342:350	composite	342:350	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	21	theme	protein	306:312	arg1	/pullulan					327:335	mung bean protein isolate (MPI)/pullulan	296:335	mung bean protein isolate (MPI)/pullulan	296:335	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	2	22	theme	scanning	536:543	arg1	microscopy					554:563	scanning electron microscopy	536:563	scanning electron microscopy (SEM)	536:569	The Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM) results confirmed the compatibility between components.					
35026220	2	22	theme	scanning	536:543	arg1	SEM					566:568	SEM	566:568	SEM	566:568	The Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM) results confirmed the compatibility between components.					
35026220	0	23	theme	essential	94:102	arg1	Origanum					72:79	Origanum	72:79	Origanum	72:79	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	0	23	theme	essential	94:102	arg1	oil					104:106	marjoram (Origanum majorana L.) essential oil	62:106	marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat	62:163	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	5	24	theme	MEO	934:936	arg1	addition					922:929	addition	922:929	addition of MEO	922:936	Also, with addition of MEO, the EAB of the antimicrobial blend-films was decreased, while TS and water-proof properties were increased.					
35026220	0	25	dep	Origanum	72:79	arg1	L.					90:91	Origanum majorana L.	72:91	Origanum majorana L.	72:91	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	1	26	from	4 °C	437:440	arg1	storage					426:432	14 days storage	418:432	14 days storage at 4 °C	418:440	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	0	27	theme	mung	10:13	arg1	bean					15:18	mung bean	10:18	mung bean protein	10:26	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	5	28	theme	TS	1001:1002	arg1	properties					1020:1029	TS and water-proof properties	1001:1029	TS and water-proof properties	1001:1029	Also, with addition of MEO, the EAB of the antimicrobial blend-films was decreased, while TS and water-proof properties were increased.					
35026220	4	29	theme	significant	832:842	arg1	increment					844:852	a significant increment	830:852	a significant increment in TS and water-proof properties of the composite films	830:908	The results showed that an increase in the level of the MPI led to a significant increment in TS and water-proof properties of the composite films.					
35026220	0	30	theme	protein	20:26	arg1	Effect					0:5	Effect	0:5	Effect of mung bean protein	0:26	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	5	31	theme	antimicrobial	954:966	arg1	blend-films					968:978	the antimicrobial blend-films	950:978	the antimicrobial blend-films	950:978	Also, with addition of MEO, the EAB of the antimicrobial blend-films was decreased, while TS and water-proof properties were increased.					
35026220	6	32	from	effect	1124:1129	arg1	activity					1176:1183	antibacterial activity	1162:1183	antibacterial activity	1162:1183	In addition, enrichment of the films with MEO led to a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli).					
35026220	6	32	from	effect	1124:1129	arg1	scavenging					1147:1156	DPPH radical scavenging	1134:1156	DPPH radical scavenging	1134:1156	In addition, enrichment of the films with MEO led to a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli).					
35026220	7	33	theme	meat	1318:1321	arg1	samples					1323:1329	the minced meat samples	1307:1329	the minced meat samples	1307:1329	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	1	34	theme	essential	204:212	arg1	MEO					219:221	MEO	219:221	MEO	219:221	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	34	theme	essential	204:212	arg1	oil					214:216	marjoram essential oil	195:216	marjoram essential oil (MEO)	195:222	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	35	theme	antioxidant	252:262	arg1	properties					282:291	antioxidant and antimicrobial properties	252:291	antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan	252:335	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	35	theme	antioxidant	252:262	arg1	barrier					243:249	barrier	243:249	barrier	243:249	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	6	36	with	enrichment	1060:1069	arg1	MEO					1089:1091	MEO	1089:1091	MEO	1089:1091	In addition, enrichment of the films with MEO led to a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli).					
35026220	5	37	theme	water-proof	1008:1018	arg1	properties					1020:1029	TS and water-proof properties	1001:1029	TS and water-proof properties	1001:1029	Also, with addition of MEO, the EAB of the antimicrobial blend-films was decreased, while TS and water-proof properties were increased.					
35026220	7	38	from	MEO-incorporation	1332:1348	arg1	films					1360:1364	MPI/PU films	1353:1364	MPI/PU films	1353:1364	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	2	39	theme	X-ray	508:512	arg1	XRD					527:529	XRD	527:529	XRD	527:529	The Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM) results confirmed the compatibility between components.					
35026220	2	39	theme	X-ray	508:512	arg1	diffraction					514:524	X-ray diffraction	508:524	X-ray diffraction (XRD)	508:530	The Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM) results confirmed the compatibility between components.					
35026220	1	40	from	influence	366:374	arg1	films					352:356	the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films	227:356	the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films	227:356	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	40	from	influence	366:374	arg1	quality					383:389	the quality	379:389	the quality of minced beef meat	379:409	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	4	41	theme	films	904:908	arg1	properties					876:885	TS and water-proof properties	857:885	TS and water-proof properties of the composite films	857:908	The results showed that an increase in the level of the MPI led to a significant increment in TS and water-proof properties of the composite films.					
35026220	0	42	contain	containing	51:60	arg2	oil					104:106	marjoram (Origanum majorana L.) essential oil	62:106	marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat	62:163	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	0	42	contain	containing	51:60	arg1	films					45:49	films	45:49	films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat	45:163	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	0	42	contain	containing	51:60	arg2	Origanum					72:79	Origanum	72:79	Origanum	72:79	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	1	43	dep	composite	342:350	arg1	properties					282:291	antioxidant and antimicrobial properties	252:291	antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan	252:335	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	43	dep	composite	342:350	arg1	barrier					243:249	barrier	243:249	barrier	243:249	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	6	44	theme	films	1078:1082	arg1	enrichment					1060:1069	enrichment	1060:1069	enrichment of the films with MEO	1060:1091	In addition, enrichment of the films with MEO led to a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli).					
35026220	2	45	dep	Fourier	459:465	arg1	transform					467:475	transform	467:475	transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM) results	467:577	The Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM) results confirmed the compatibility between components.					
35026220	0	46	theme	chemical	111:118	arg1	properties					134:143	chemical and microbial properties	111:143	chemical and microbial properties of minced beef meat	111:163	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	1	47	theme	beef	401:404	arg1	meat					406:409	minced beef meat	394:409	minced beef meat	394:409	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	3	48	from	break	738:742	arg1	at					735:736	elongation at break	724:742	elongation at break (EAB)	724:748	Also, depend on the different ratios of combination of MEO and MPI/PU, tensile strength (TS) and elongation at break (EAB) were varied.					
35026220	3	48	from	break	738:742	arg1	elongation					724:733	elongation at break	724:742	elongation at break (EAB)	724:748	Also, depend on the different ratios of combination of MEO and MPI/PU, tensile strength (TS) and elongation at break (EAB) were varied.					
35026220	0	49	theme	microbial	124:132	arg1	properties					134:143	chemical and microbial properties	111:143	chemical and microbial properties of minced beef meat	111:163	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	1	50	theme	/pullulan	327:335	arg1	properties					282:291	antioxidant and antimicrobial properties	252:291	antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan	252:335	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	50	theme	/pullulan	327:335	arg1	barrier					243:249	barrier	243:249	barrier	243:249	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	2	51	dep	spectroscopy	494:505	arg1	results					571:577	results	571:577	results	571:577	The Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM) results confirmed the compatibility between components.					
35026220	7	52	theme	effective	1435:1443	arg1	activity					1459:1466	effective antimicrobial activity	1435:1466	effective antimicrobial activity	1435:1466	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	6	53	theme	DPPH	1134:1137	arg1	scavenging					1147:1156	DPPH radical scavenging	1134:1156	DPPH radical scavenging	1134:1156	In addition, enrichment of the films with MEO led to a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli).					
35026220	3	54	theme	MEO	682:684	arg1	combination					667:677	combination	667:677	combination of MEO and MPI/PU	667:695	Also, depend on the different ratios of combination of MEO and MPI/PU, tensile strength (TS) and elongation at break (EAB) were varied.					
35026220	1	55	from	effect	185:190	arg1	films					352:356	the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films	227:356	the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films	227:356	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	55	from	effect	185:190	arg1	quality					383:389	the quality	379:389	the quality of minced beef meat	379:409	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	2	56	theme	FT-IR	487:491	arg1	spectroscopy					494:505	infrared (FT-IR) spectroscopy	477:505	infrared (FT-IR) spectroscopy	477:505	The Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM) results confirmed the compatibility between components.					
35026220	0	57	from	oil	104:106	arg1	properties					134:143	chemical and microbial properties	111:143	chemical and microbial properties of minced beef meat	111:163	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	3	58	theme	combination	667:677	arg1	ratios					657:662	the different ratios	643:662	the different ratios of combination of MEO and MPI/PU	643:695	Also, depend on the different ratios of combination of MEO and MPI/PU, tensile strength (TS) and elongation at break (EAB) were varied.					
35026220	0	59	theme	beef	155:158	arg1	meat					160:163	minced beef meat	148:163	minced beef meat	148:163	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	2	60	theme	infrared	477:484	arg1	spectroscopy					494:505	infrared (FT-IR) spectroscopy	477:505	infrared (FT-IR) spectroscopy	477:505	The Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM) results confirmed the compatibility between components.					
35026220	3	61	theme	tensile	698:704	arg1	TS					716:717	TS	716:717	TS	716:717	Also, depend on the different ratios of combination of MEO and MPI/PU, tensile strength (TS) and elongation at break (EAB) were varied.					
35026220	3	61	theme	tensile	698:704	arg1	strength					706:713	tensile strength	698:713	tensile strength (TS)	698:718	Also, depend on the different ratios of combination of MEO and MPI/PU, tensile strength (TS) and elongation at break (EAB) were varied.					
35026220	3	62	theme	elongation	724:733	arg1	EAB					745:747	EAB	745:747	EAB	745:747	Also, depend on the different ratios of combination of MEO and MPI/PU, tensile strength (TS) and elongation at break (EAB) were varied.					
35026220	3	62	theme	elongation	724:733	arg1	break					738:742	elongation at break	724:742	elongation at break (EAB)	724:748	Also, depend on the different ratios of combination of MEO and MPI/PU, tensile strength (TS) and elongation at break (EAB) were varied.					
35026220	7	63	theme	beef	1405:1408	arg1	samples					1410:1416	minced beef samples	1398:1416	minced beef samples	1398:1416	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	3	64	theme	MPI/PU	690:695	arg1	combination					667:677	combination	667:677	combination of MEO and MPI/PU	667:695	Also, depend on the different ratios of combination of MEO and MPI/PU, tensile strength (TS) and elongation at break (EAB) were varied.					
35026220	1	65	theme	bean	301:304	arg1	/pullulan					327:335	mung bean protein isolate (MPI)/pullulan	296:335	mung bean protein isolate (MPI)/pullulan	296:335	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	7	66	theme	oxidative	1375:1383	arg1	stability					1385:1393	oxidative stability	1375:1393	oxidative stability of minced beef samples	1375:1416	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	2	67	theme	electron	545:552	arg1	microscopy					554:563	scanning electron microscopy	536:563	scanning electron microscopy (SEM)	536:569	The Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM) results confirmed the compatibility between components.					
35026220	2	67	theme	electron	545:552	arg1	SEM					566:568	SEM	566:568	SEM	566:568	The Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM) results confirmed the compatibility between components.					
35026220	6	68	theme	antibacterial	1162:1174	arg1	activity					1176:1183	antibacterial activity	1162:1183	antibacterial activity	1162:1183	In addition, enrichment of the films with MEO led to a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli).					
35026220	1	69	theme	isolate	314:320	arg1	/pullulan					327:335	mung bean protein isolate (MPI)/pullulan	296:335	mung bean protein isolate (MPI)/pullulan	296:335	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	6	70	dep	bacteria	1204:1211	arg1	aureus					1229:1234	Staphylococcus aureus	1214:1234	Staphylococcus aureus	1214:1234	In addition, enrichment of the films with MEO led to a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli).					
35026220	6	70	dep	bacteria	1204:1211	arg1	coli					1252:1255	Escherichia coli	1240:1255	Escherichia coli	1240:1255	In addition, enrichment of the films with MEO led to a considerable positive effect on DPPH radical scavenging and antibacterial activity against pathogenic bacteria (Staphylococcus aureus and Escherichia coli).					
35026220	1	71	theme	MPI	323:325	arg1	/pullulan					327:335	mung bean protein isolate (MPI)/pullulan	296:335	mung bean protein isolate (MPI)/pullulan	296:335	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	4	72	theme	TS	857:858	arg1	properties					876:885	TS and water-proof properties	857:885	TS and water-proof properties of the composite films	857:908	The results showed that an increase in the level of the MPI led to a significant increment in TS and water-proof properties of the composite films.					
35026220	0	73	theme	bean	15:18	arg1	protein					20:26	mung bean protein	10:26	mung bean protein	10:26	Effect of mung bean protein isolate/pullulan films containing marjoram (Origanum majorana L.) essential oil on chemical and microbial properties of minced beef meat.					
35026220	1	74	theme	mechanical	231:240	arg1	films					352:356	the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films	227:356	the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films	227:356	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	4	75	theme	water-proof	864:874	arg1	properties					876:885	TS and water-proof properties	857:885	TS and water-proof properties of the composite films	857:908	The results showed that an increase in the level of the MPI led to a significant increment in TS and water-proof properties of the composite films.					
35026220	7	76	theme	tested	1487:1492	arg1	bacteria					1494:1501	the tested bacteria	1483:1501	the tested bacteria	1483:1501	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	1	77	theme	meat	406:409	arg1	quality					383:389	the quality	379:389	the quality of minced beef meat	379:409	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	5	78	theme	blend-films	968:978	arg1	EAB					943:945	the EAB	939:945	the EAB of the antimicrobial blend-films	939:978	Also, with addition of MEO, the EAB of the antimicrobial blend-films was decreased, while TS and water-proof properties were increased.					
35026220	7	79	theme	MPI/PU	1353:1358	arg1	films					1360:1364	MPI/PU films	1353:1364	MPI/PU films	1353:1364	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	1	80	theme	14 days	418:424	arg1	storage					426:432	14 days storage	418:432	14 days storage at 4 °C	418:440	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	81	theme	antimicrobial	268:280	arg1	properties					282:291	antioxidant and antimicrobial properties	252:291	antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan	252:335	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	81	theme	antimicrobial	268:280	arg1	barrier					243:249	barrier	243:249	barrier	243:249	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	82	theme	marjoram	195:202	arg1	MEO					219:221	MEO	219:221	MEO	219:221	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	82	theme	marjoram	195:202	arg1	oil					214:216	marjoram essential oil	195:216	marjoram essential oil (MEO)	195:222	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	7	83	theme	samples	1323:1329	arg1	analyses					1295:1302	the bacterial and chemical analyses	1268:1302	the bacterial and chemical analyses of the minced meat samples	1268:1329	Based on the bacterial and chemical analyses of the minced meat samples, MEO-incorporation in MPI/PU films enhanced oxidative stability of minced beef samples, and also showed effective antimicrobial activity against all of the tested bacteria.					
35026220	4	84	from	increment	844:852	arg1	properties					876:885	TS and water-proof properties	857:885	TS and water-proof properties of the composite films	857:908	The results showed that an increase in the level of the MPI led to a significant increment in TS and water-proof properties of the composite films.					
35026220	1	85	theme	oil	214:216	arg1	influence					366:374	its influence	362:374	its influence on the quality of minced beef meat during 14 days storage at 4 °C	362:440	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
35026220	1	85	theme	oil	214:216	arg1	effect					185:190	the effect	181:190	the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films	181:356	In this study, the effect of marjoram essential oil (MEO) on the mechanical, barrier, antioxidant and antimicrobial properties of mung bean protein isolate (MPI)/pullulan (PU) composite films and its influence on the quality of minced beef meat during 14 days storage at 4 °C was studied.					
36604578	2	0	theme	Normalized	486:495	arg1	NDVI					526:529	NDVI	526:529	NDVI	526:529	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	2	0	theme	Normalized	486:495	arg1	index					519:523	Normalized Difference Vegetation index	486:523	Normalized Difference Vegetation index	486:523	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	1	1	from	benefits	185:192	arg1	children					197:204	children	197:204	children	197:204	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	3	2	theme	HMOs	816:819	arg1	concentrations					779:792	concentrations	779:792	concentrations of several individual HMOs and HMO groups	779:834	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	3	2	theme	HMOs	816:819	arg1	increases					765:773	HMO diversity increases	751:773	HMO diversity increases	751:773	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	5	3	theme	mediating	1071:1079	arg1	role					1081:1084	the mediating role	1067:1084	the mediating role of breastfeeding between the residential green environments	1067:1144	The results emphasize the mediating role of breastfeeding between the residential green environments and health in early life.					
36604578	2	4	dep	Vegetation	537:546	arg1	2					534:534	2	534:534	2	534:534	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	2	4	dep	Vegetation	537:546	arg1	Cover					548:552	Cover	548:552	Cover Diversity (VCDI)	548:569	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	4	5	theme	maternal	997:1004	arg1	composition					1032:1042	maternal milk through modified HMO composition	997:1042	maternal milk through modified HMO composition	997:1042	This suggests that variation in residential green environments may influence the infant via maternal milk through modified HMO composition.					
36604578	3	6	theme	HMO	751:753	arg1	increases					765:773	HMO diversity increases	751:773	HMO diversity increases	751:773	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	2	7	dep	greenness	475:483	arg1	1					472:472	1	472:472	1	472:472	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	2	7	dep	greenness	475:483	arg1	NDVI					526:529	NDVI	526:529	NDVI	526:529	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	2	7	dep	greenness	475:483	arg1	index					519:523	Normalized Difference Vegetation index	486:523	Normalized Difference Vegetation index	486:523	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	3	8	from	VCDI	858:861	arg1	environments					891:902	residential green environments	873:902	residential green environments	873:902	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	3	9	theme	several	797:803	arg1	HMOs					816:819	several individual HMOs	797:819	several individual HMOs	797:819	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	4	10	theme	residential	937:947	arg1	environments					955:966	residential green environments	937:966	residential green environments	937:966	This suggests that variation in residential green environments may influence the infant via maternal milk through modified HMO composition.					
36604578	3	11	theme	groups	829:834	arg1	concentrations					779:792	concentrations	779:792	concentrations of several individual HMOs and HMO groups	779:834	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	3	11	theme	groups	829:834	arg1	increases					765:773	HMO diversity increases	751:773	HMO diversity increases	751:773	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	1	12	theme	biological	269:278	arg1	mediators					280:288	biological mediators	269:288	biological mediators such as altered maternal milk composition	269:330	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	1	12	theme	biological	269:278	arg1	composition					320:330	altered maternal milk composition	298:330	altered maternal milk composition	298:330	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	4	13	theme	HMO	1028:1030	arg1	composition					1032:1042	maternal milk through modified HMO composition	997:1042	maternal milk through modified HMO composition	997:1042	This suggests that variation in residential green environments may influence the infant via maternal milk through modified HMO composition.					
36604578	2	14	theme	health	690:695	arg1	effects					697:703	their immune- and microbiota-related health effects	653:703	their immune- and microbiota-related health effects on the infant (N = 795 mothers)	653:735	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	1	15	theme	health	178:183	arg1	benefits					185:192	health benefits	178:192	health benefits in children	178:204	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	4	16	theme	green	949:953	arg1	environments					955:966	residential green environments	937:966	residential green environments	937:966	This suggests that variation in residential green environments may influence the infant via maternal milk through modified HMO composition.					
36604578	2	17	theme	microbiota-related	671:688	arg1	effects					697:703	their immune- and microbiota-related health effects	653:703	their immune- and microbiota-related health effects on the infant (N = 795 mothers)	653:735	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	0	18	theme	green	12:16	arg1	environments					18:29	Residential green environments	0:29	Residential green environments	0:29	Residential green environments are associated with human milk oligosaccharide diversity and composition.					
36604578	2	19	theme	milk	613:616	arg1	HMOs					636:639	HMOs	636:639	HMOs	636:639	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	2	19	theme	milk	613:616	arg1	oligosaccharides					618:633	human milk oligosaccharides	607:633	human milk oligosaccharides (HMOs)	607:640	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	3	20	theme	HMO	825:827	arg1	groups					829:834	HMO groups	825:834	HMO groups	825:834	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	0	21	theme	Residential	0:10	arg1	environments					18:29	Residential green environments	0:29	Residential green environments	0:29	Residential green environments are associated with human milk oligosaccharide diversity and composition.					
36604578	5	22	theme	residential	1115:1125	arg1	environments					1133:1144	the residential green environments	1111:1144	the residential green environments	1111:1144	The results emphasize the mediating role of breastfeeding between the residential green environments and health in early life.					
36604578	2	23	theme	human	607:611	arg1	HMOs					636:639	HMOs	636:639	HMOs	636:639	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	2	23	theme	human	607:611	arg1	oligosaccharides					618:633	human milk oligosaccharides	607:633	human milk oligosaccharides (HMOs)	607:640	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	1	24	theme	Increased	105:113	arg1	exposure					115:122	Increased exposure	105:122	Increased exposure to greener environments	105:146	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	4	25	theme	modified	1019:1026	arg1	composition					1032:1042	maternal milk through modified HMO composition	997:1042	maternal milk through modified HMO composition	997:1042	This suggests that variation in residential green environments may influence the infant via maternal milk through modified HMO composition.					
36604578	2	26	dep	Index	591:595	arg1	3					576:576	3	576:576	3	576:576	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	5	27	theme	green	1127:1131	arg1	environments					1133:1144	the residential green environments	1111:1144	the residential green environments	1111:1144	The results emphasize the mediating role of breastfeeding between the residential green environments and health in early life.					
36604578	1	28	dep	suggested	157:165	arg1	lead					170:173	lead	170:173	has been suggested to lead to health benefits in children	148:204	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	2	29	theme	green	440:444	arg1	environment					446:456	the mother's residential green environment	415:456	the mother's residential green environment	415:456	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	2	30	theme	residential	428:438	arg1	environment					446:456	the mother's residential green environment	415:456	the mother's residential green environment	415:456	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	1	31	theme	altered	298:304	arg1	composition					320:330	altered maternal milk composition	298:330	altered maternal milk composition	298:330	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	3	32	theme	increased	848:856	arg1	VCDI					858:861	VCDI	858:861	VCDI	858:861	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	1	33	theme	maternal	306:313	arg1	composition					320:330	altered maternal milk composition	298:330	altered maternal milk composition	298:330	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	3	34	theme	residential	873:883	arg1	environments					891:902	residential green environments	873:902	residential green environments	873:902	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	1	35	theme	associated	215:224	arg1	mechanisms					226:235	the associated mechanisms	211:235	the associated mechanisms	211:235	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	0	36	theme	milk	57:60	arg1	diversity					78:86	human milk oligosaccharide diversity	51:86	human milk oligosaccharide diversity	51:86	Residential green environments are associated with human milk oligosaccharide diversity and composition.					
36604578	1	37	theme	milk	315:318	arg1	composition					320:330	altered maternal milk composition	298:330	altered maternal milk composition	298:330	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	0	38	theme	oligosaccharide	62:76	arg1	diversity					78:86	human milk oligosaccharide diversity	51:86	human milk oligosaccharide diversity	51:86	Residential green environments are associated with human milk oligosaccharide diversity and composition.					
36604578	2	39	from	effects	697:703	arg1	infant					712:717	the infant	708:717	the infant (N = 795 mothers)	708:735	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	2	39	from	effects	697:703	arg1	mothers					728:734	N = 795 mothers	720:734	N = 795 mothers	720:734	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	3	40	theme	green	885:889	arg1	environments					891:902	residential green environments	873:902	residential green environments	873:902	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	1	41	theme	greener	127:133	arg1	environments					135:146	greener environments	127:146	greener environments	127:146	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	0	42	theme	human	51:55	arg1	diversity					78:86	human milk oligosaccharide diversity	51:86	human milk oligosaccharide diversity	51:86	Residential green environments are associated with human milk oligosaccharide diversity and composition.					
36604578	4	43	from	variation	924:932	arg1	environments					955:966	residential green environments	937:966	residential green environments	937:966	This suggests that variation in residential green environments may influence the infant via maternal milk through modified HMO composition.					
36604578	4	44	theme	through	1011:1017	arg1	composition					1032:1042	maternal milk through modified HMO composition	997:1042	maternal milk through modified HMO composition	997:1042	This suggests that variation in residential green environments may influence the infant via maternal milk through modified HMO composition.					
36604578	1	45	from	mechanisms	226:235	arg1	life					246:249	early life	240:249	early life	240:249	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	5	46	theme	early	1160:1164	arg1	life					1166:1169	early life	1160:1169	early life	1160:1169	The results emphasize the mediating role of breastfeeding between the residential green environments and health in early life.					
36604578	4	47	theme	milk	1006:1009	arg1	composition					1032:1042	maternal milk through modified HMO composition	997:1042	maternal milk through modified HMO composition	997:1042	This suggests that variation in residential green environments may influence the infant via maternal milk through modified HMO composition.					
36604578	2	48	theme	immune-	659:665	arg1	effects					697:703	their immune- and microbiota-related health effects	653:703	their immune- and microbiota-related health effects on the infant (N = 795 mothers)	653:735	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	3	49	theme	diversity	755:763	arg1	increases					765:773	HMO diversity increases	751:773	HMO diversity increases	751:773	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	2	50	theme	Vegetation	508:517	arg1	NDVI					526:529	NDVI	526:529	NDVI	526:529	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	2	50	theme	Vegetation	508:517	arg1	index					519:523	Normalized Difference Vegetation index	486:523	Normalized Difference Vegetation index	486:523	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	3	51	from	NI	867:868	arg1	environments					891:902	residential green environments	873:902	residential green environments	873:902	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	2	52	theme	N = 795	720:726	arg1	infant					712:717	the infant	708:717	the infant (N = 795 mothers)	708:735	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	2	52	theme	N = 795	720:726	arg1	mothers					728:734	N = 795 mothers	720:734	N = 795 mothers	720:734	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	1	53	theme	early	240:244	arg1	life					246:249	early life	240:249	early life	240:249	Increased exposure to greener environments has been suggested to lead to health benefits in children, but the associated mechanisms in early life, particularly via biological mediators such as altered maternal milk composition, remain largely unexplored.					
36604578	2	54	theme	Difference	497:506	arg1	NDVI					526:529	NDVI	526:529	NDVI	526:529	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	2	54	theme	Difference	497:506	arg1	index					519:523	Normalized Difference Vegetation index	486:523	Normalized Difference Vegetation index	486:523	We investigated the associations between properties of the mother's residential green environment, measured as (1) greenness (Normalized Difference Vegetation index, NDVI), (2) Vegetation Cover Diversity (VCDI) and (3) Naturalness Index (NI), and human milk oligosaccharides (HMOs), known for their immune- and microbiota-related health effects on the infant (N = 795 mothers).					
36604578	3	55	theme	individual	805:814	arg1	HMOs					816:819	several individual HMOs	797:819	several individual HMOs	797:819	We show that HMO diversity increases and concentrations of several individual HMOs and HMO groups change with increased VCDI and NI in residential green environments.					
36604578	5	56	theme	breastfeeding	1089:1101	arg1	health					1150:1155	health	1150:1155	health	1150:1155	The results emphasize the mediating role of breastfeeding between the residential green environments and health in early life.					
36604578	5	56	theme	breastfeeding	1089:1101	arg1	role					1081:1084	the mediating role	1067:1084	the mediating role of breastfeeding between the residential green environments	1067:1144	The results emphasize the mediating role of breastfeeding between the residential green environments and health in early life.					
37267648	0	0	theme	processed	91:99	arg1	pulp					112:115	thermal and non-thermal processed strawberry pulp	67:115	thermal and non-thermal processed strawberry pulp	67:115	Pectin-interactions and the digestive stability of anthocyanins in thermal and non-thermal processed strawberry pulp.					
37267648	1	1	theme	pectin	365:370	arg1	HP					499:500	HP	499:500	HP	499:500	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	1	theme	pectin	365:370	arg1	PS					426:427	PS	426:427	PS	426:427	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	1	theme	pectin	365:370	arg1	pulp					392:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp	340:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS)	340:428	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	2	with	anthocyanins	169:180	arg1	pectin					253:258	three pectin fractions-water-soluble pectin	216:258	three pectin fractions-water-soluble pectin (WSP)	216:264	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	2	with	anthocyanins	169:180	arg1	HP					499:500	HP	499:500	HP	499:500	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	2	with	anthocyanins	169:180	arg1	WSP					261:263	WSP	261:263	WSP	261:263	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	2	with	anthocyanins	169:180	arg1	pectin					322:327	cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin	267:327	cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP)	267:333	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	2	with	anthocyanins	169:180	arg1	PS					426:427	PS	426:427	PS	426:427	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	2	with	anthocyanins	169:180	arg1	pulp					392:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp	340:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS)	340:428	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	2	with	anthocyanins	169:180	arg1	CSP					330:332	CSP	330:332	CSP	330:332	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	2	3	theme	simulated	639:647	arg1	intestine					649:657	the simulated intestine	635:657	the simulated intestine	635:657	Compared with the control group, the ACNs content increased to the highest level (312.89 mg/mL), but the retention rate of ACNs in the simulated intestine decreased significantly after US treatment.					
37267648	1	4	theme	anthocyanins	169:180	arg1	stability					156:164	the digestive stability	142:164	the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP)	142:501	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	3	5	theme	more	776:779	arg1	domains					803:809	more homogalacturonan (HG) domains	776:809	more homogalacturonan (HG) domains	776:809	The monosaccharide compositions indicated that the WSP and CSP possessed more homogalacturonan (HG) domains than the NSP, which contains more rhamngalacturonan-I (RG-I) domains.					
37267648	0	6	theme	non-thermal	79:89	arg1	pulp					112:115	thermal and non-thermal processed strawberry pulp	67:115	thermal and non-thermal processed strawberry pulp	67:115	Pectin-interactions and the digestive stability of anthocyanins in thermal and non-thermal processed strawberry pulp.					
37267648	1	7	dep	ultrasound	431:440	arg1	US					443:444	US	443:444	US	443:444	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	8	theme	NSP	373:375	arg1	HP					499:500	HP	499:500	HP	499:500	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	8	theme	NSP	373:375	arg1	PS					426:427	PS	426:427	PS	426:427	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	8	theme	NSP	373:375	arg1	pulp					392:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp	340:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS)	340:428	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	2	9	theme	ACNs	541:544	arg1	content					546:552	the ACNs content	537:552	the ACNs content	537:552	Compared with the control group, the ACNs content increased to the highest level (312.89 mg/mL), but the retention rate of ACNs in the simulated intestine decreased significantly after US treatment.					
37267648	1	10	theme	cyclohexanetrans-1,2-diamine	267:294	arg1	CSP					330:332	CSP	330:332	CSP	330:332	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	10	theme	cyclohexanetrans-1,2-diamine	267:294	arg1	pectin					322:327	cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin	267:327	cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP)	267:333	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	0	11	theme	strawberry	101:110	arg1	pulp					112:115	thermal and non-thermal processed strawberry pulp	67:115	thermal and non-thermal processed strawberry pulp	67:115	Pectin-interactions and the digestive stability of anthocyanins in thermal and non-thermal processed strawberry pulp.					
37267648	6	12	theme	pulp	1225:1228	arg1	processing					1230:1239	pulp processing	1225:1239	pulp processing	1225:1239	These results revealed the structure-activity relationship between ACNs and pectin during pulp processing.					
37267648	2	13	from	rate	619:622	arg1	intestine					649:657	the simulated intestine	635:657	the simulated intestine	635:657	Compared with the control group, the ACNs content increased to the highest level (312.89 mg/mL), but the retention rate of ACNs in the simulated intestine decreased significantly after US treatment.					
37267648	1	14	theme	tetra-acetic	296:307	arg1	CSP					330:332	CSP	330:332	CSP	330:332	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	14	theme	tetra-acetic	296:307	arg1	pectin					322:327	cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin	267:327	cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP)	267:333	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	15	theme	-in	377:379	arg1	HP					499:500	HP	499:500	HP	499:500	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	15	theme	-in	377:379	arg1	PS					426:427	PS	426:427	PS	426:427	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	15	theme	-in	377:379	arg1	pulp					392:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp	340:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS)	340:428	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	3	16	theme	rhamngalacturonan-I	845:863	arg1	domains					872:878	more rhamngalacturonan-I (RG-I) domains	840:878	more rhamngalacturonan-I (RG-I) domains	840:878	The monosaccharide compositions indicated that the WSP and CSP possessed more homogalacturonan (HG) domains than the NSP, which contains more rhamngalacturonan-I (RG-I) domains.					
37267648	0	17	theme	anthocyanins	51:62	arg1	Pectin-interactions					0:18	Pectin-interactions	0:18	Pectin-interactions	0:18	Pectin-interactions and the digestive stability of anthocyanins in thermal and non-thermal processed strawberry pulp.					
37267648	0	17	theme	anthocyanins	51:62	arg1	stability					38:46	the digestive stability	24:46	the digestive stability of anthocyanins in thermal and non-thermal processed strawberry pulp	24:115	Pectin-interactions and the digestive stability of anthocyanins in thermal and non-thermal processed strawberry pulp.					
37267648	1	18	theme	strawberry	381:390	arg1	HP					499:500	HP	499:500	HP	499:500	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	18	theme	strawberry	381:390	arg1	PS					426:427	PS	426:427	PS	426:427	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	18	theme	strawberry	381:390	arg1	pulp					392:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp	340:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS)	340:428	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	2	19	theme	highest	571:577	arg1	312.89 mg/mL					586:597	312.89 mg/mL	586:597	312.89 mg/mL	586:597	Compared with the control group, the ACNs content increased to the highest level (312.89 mg/mL), but the retention rate of ACNs in the simulated intestine decreased significantly after US treatment.					
37267648	2	19	theme	highest	571:577	arg1	level					579:583	the highest level	567:583	the highest level (312.89 mg/mL)	567:598	Compared with the control group, the ACNs content increased to the highest level (312.89 mg/mL), but the retention rate of ACNs in the simulated intestine decreased significantly after US treatment.					
37267648	1	20	theme	electron	448:455	arg1	irradiation					467:477	electron beam (EB) irradiation	448:477	electron beam (EB) irradiation	448:477	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	2	21	theme	US	689:690	arg1	treatment					692:700	US treatment	689:700	US treatment	689:700	Compared with the control group, the ACNs content increased to the highest level (312.89 mg/mL), but the retention rate of ACNs in the simulated intestine decreased significantly after US treatment.					
37267648	0	22	from	stability	38:46	arg1	pulp					112:115	thermal and non-thermal processed strawberry pulp	67:115	thermal and non-thermal processed strawberry pulp	67:115	Pectin-interactions and the digestive stability of anthocyanins in thermal and non-thermal processed strawberry pulp.					
37267648	1	23	theme	beam	457:460	arg1	irradiation					467:477	electron beam (EB) irradiation	448:477	electron beam (EB) irradiation	448:477	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	5	24	theme	pectin	1038:1043	arg1	structure					1045:1053	the pectin structure	1034:1053	the pectin structure	1034:1053	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	1	25	theme	acid-soluble	309:320	arg1	CSP					330:332	CSP	330:332	CSP	330:332	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	25	theme	acid-soluble	309:320	arg1	pectin					322:327	cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin	267:327	cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP)	267:333	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	0	26	theme	digestive	28:36	arg1	stability					38:46	the digestive stability	24:46	the digestive stability of anthocyanins in thermal and non-thermal processed strawberry pulp	24:115	Pectin-interactions and the digestive stability of anthocyanins in thermal and non-thermal processed strawberry pulp.					
37267648	1	27	theme	EB	463:464	arg1	irradiation					467:477	electron beam (EB) irradiation	448:477	electron beam (EB) irradiation	448:477	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	28	theme	interaction	199:209	arg1	stability					156:164	the digestive stability	142:164	the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP)	142:501	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	5	29	theme	structure	1124:1132	arg1	integrity					1111:1119	the integrity	1107:1119	the integrity of structure	1107:1132	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	5	29	theme	structure	1124:1132	arg1	degree					1083:1088	the degree	1079:1088	the degree of linearity	1079:1101	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	1	30	with	interaction	199:209	arg1	pectin					253:258	three pectin fractions-water-soluble pectin	216:258	three pectin fractions-water-soluble pectin (WSP)	216:264	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	30	with	interaction	199:209	arg1	HP					499:500	HP	499:500	HP	499:500	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	30	with	interaction	199:209	arg1	WSP					261:263	WSP	261:263	WSP	261:263	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	30	with	interaction	199:209	arg1	pectin					322:327	cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin	267:327	cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP)	267:333	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	30	with	interaction	199:209	arg1	PS					426:427	PS	426:427	PS	426:427	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	30	with	interaction	199:209	arg1	pulp					392:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp	340:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS)	340:428	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	30	with	interaction	199:209	arg1	CSP					330:332	CSP	330:332	CSP	330:332	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	3	31	contain	possessed	766:774	arg1	WSP					754:756	WSP	754:756	WSP	754:756	The monosaccharide compositions indicated that the WSP and CSP possessed more homogalacturonan (HG) domains than the NSP, which contains more rhamngalacturonan-I (RG-I) domains.					
37267648	3	31	contain	possessed	766:774	arg2	domains					803:809	more homogalacturonan (HG) domains	776:809	more homogalacturonan (HG) domains	776:809	The monosaccharide compositions indicated that the WSP and CSP possessed more homogalacturonan (HG) domains than the NSP, which contains more rhamngalacturonan-I (RG-I) domains.					
37267648	3	31	contain	possessed	766:774	arg1	CSP					762:764	CSP	762:764	CSP	762:764	The monosaccharide compositions indicated that the WSP and CSP possessed more homogalacturonan (HG) domains than the NSP, which contains more rhamngalacturonan-I (RG-I) domains.					
37267648	3	32	theme	homogalacturonan	781:796	arg1	domains					803:809	more homogalacturonan (HG) domains	776:809	more homogalacturonan (HG) domains	776:809	The monosaccharide compositions indicated that the WSP and CSP possessed more homogalacturonan (HG) domains than the NSP, which contains more rhamngalacturonan-I (RG-I) domains.					
37267648	1	33	theme	pectin	222:227	arg1	WSP					261:263	WSP	261:263	WSP	261:263	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	33	theme	pectin	222:227	arg1	pectin					253:258	three pectin fractions-water-soluble pectin	216:258	three pectin fractions-water-soluble pectin (WSP)	216:264	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	3	34	contain	contains	831:838	arg2	domains					872:878	more rhamngalacturonan-I (RG-I) domains	840:878	more rhamngalacturonan-I (RG-I) domains	840:878	The monosaccharide compositions indicated that the WSP and CSP possessed more homogalacturonan (HG) domains than the NSP, which contains more rhamngalacturonan-I (RG-I) domains.					
37267648	3	34	contain	contains	831:838	arg1	NSP					820:822	the NSP	816:822	the NSP	816:822	The monosaccharide compositions indicated that the WSP and CSP possessed more homogalacturonan (HG) domains than the NSP, which contains more rhamngalacturonan-I (RG-I) domains.					
37267648	4	35	theme	US-treated	903:912	arg1	pectin					914:919	US-treated pectin	903:919	US-treated pectin	903:919	The microstructure of US-treated pectin was damaged and fragmented.					
37267648	6	36	theme	structure-activity	1162:1179	arg1	relationship					1181:1192	the structure-activity relationship	1158:1192	the structure-activity relationship between ACNs and pectin during pulp processing	1158:1239	These results revealed the structure-activity relationship between ACNs and pectin during pulp processing.					
37267648	0	37	from	Pectin-interactions	0:18	arg1	pulp					112:115	thermal and non-thermal processed strawberry pulp	67:115	thermal and non-thermal processed strawberry pulp	67:115	Pectin-interactions and the digestive stability of anthocyanins in thermal and non-thermal processed strawberry pulp.					
37267648	5	38	theme	linearity	1093:1101	arg1	integrity					1111:1119	the integrity	1107:1119	the integrity of structure	1107:1132	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	5	38	theme	linearity	1093:1101	arg1	degree					1083:1088	the degree	1079:1088	the degree of linearity	1079:1101	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	5	39	theme	retention	988:996	arg1	reflected					1066:1074	reflected	1066:1074	reflected by the degree of linearity and the integrity of structure	1066:1132	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	5	39	theme	retention	988:996	arg1	related					1023:1029	related	1023:1029	related	1023:1029	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	5	39	theme	retention	988:996	arg1	rate					998:1001	the retention rate	984:1001	the retention rate of ACNs	984:1009	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	2	40	theme	ACNs	627:630	arg1	rate					619:622	the retention rate	605:622	the retention rate of ACNs in the simulated intestine	605:657	Compared with the control group, the ACNs content increased to the highest level (312.89 mg/mL), but the retention rate of ACNs in the simulated intestine decreased significantly after US treatment.					
37267648	1	41	theme	sodium	340:345	arg1	HP					499:500	HP	499:500	HP	499:500	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	41	theme	sodium	340:345	arg1	PS					426:427	PS	426:427	PS	426:427	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	41	theme	sodium	340:345	arg1	pulp					392:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp	340:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS)	340:428	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	2	42	theme	control	522:528	arg1	group					530:534	the control group	518:534	the control group	518:534	Compared with the control group, the ACNs content increased to the highest level (312.89 mg/mL), but the retention rate of ACNs in the simulated intestine decreased significantly after US treatment.					
37267648	3	43	dep	rhamngalacturonan-I	845:863	arg1	RG-I					866:869	RG-I	866:869	RG-I	866:869	The monosaccharide compositions indicated that the WSP and CSP possessed more homogalacturonan (HG) domains than the NSP, which contains more rhamngalacturonan-I (RG-I) domains.					
37267648	1	44	theme	fractions-water-soluble	229:251	arg1	WSP					261:263	WSP	261:263	WSP	261:263	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	44	theme	fractions-water-soluble	229:251	arg1	pectin					253:258	three pectin fractions-water-soluble pectin	216:258	three pectin fractions-water-soluble pectin (WSP)	216:264	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	3	45	theme	monosaccharide	707:720	arg1	compositions					722:733	The monosaccharide compositions	703:733	The monosaccharide compositions	703:733	The monosaccharide compositions indicated that the WSP and CSP possessed more homogalacturonan (HG) domains than the NSP, which contains more rhamngalacturonan-I (RG-I) domains.					
37267648	1	46	theme	high	484:487	arg1	pressure					489:496	high pressure	484:496	high pressure	484:496	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	47	theme	digestive	146:154	arg1	stability					156:164	the digestive stability	142:164	the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP)	142:501	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	2	48	theme	retention	609:617	arg1	rate					619:622	the retention rate	605:622	the retention rate of ACNs in the simulated intestine	605:657	Compared with the control group, the ACNs content increased to the highest level (312.89 mg/mL), but the retention rate of ACNs in the simulated intestine decreased significantly after US treatment.					
37267648	4	49	theme	pectin	914:919	arg1	microstructure					885:898	The microstructure	881:898	The microstructure of US-treated pectin	881:919	The microstructure of US-treated pectin was damaged and fragmented.					
37267648	4	49	theme	pectin	914:919	arg1	damaged					925:931	damaged	925:931	damaged	925:931	The microstructure of US-treated pectin was damaged and fragmented.					
37267648	5	50	theme	ACNs	1006:1009	arg1	reflected					1066:1074	reflected	1066:1074	reflected by the degree of linearity and the integrity of structure	1066:1132	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	5	50	theme	ACNs	1006:1009	arg1	related					1023:1029	related	1023:1029	related	1023:1029	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	5	50	theme	ACNs	1006:1009	arg1	rate					998:1001	the retention rate	984:1001	the retention rate of ACNs	984:1009	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	1	51	dep	processed	397:405	arg1	PS					426:427	PS	426:427	PS	426:427	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	51	dep	processed	397:405	arg1	pulp					392:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp	340:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS)	340:428	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	0	52	theme	thermal	67:73	arg1	pulp					112:115	thermal and non-thermal processed strawberry pulp	67:115	thermal and non-thermal processed strawberry pulp	67:115	Pectin-interactions and the digestive stability of anthocyanins in thermal and non-thermal processed strawberry pulp.					
37267648	1	53	theme	carbonate-soluble	347:363	arg1	HP					499:500	HP	499:500	HP	499:500	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	53	theme	carbonate-soluble	347:363	arg1	PS					426:427	PS	426:427	PS	426:427	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	1	53	theme	carbonate-soluble	347:363	arg1	pulp					392:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp	340:395	sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS)	340:428	This study investigated the digestive stability of anthocyanins (ACNs) and their interaction with three pectin fractions-water-soluble pectin (WSP), cyclohexanetrans-1,2-diamine tetra-acetic acid-soluble pectin (CSP), and sodium carbonate-soluble pectin (NSP)-in strawberry pulp processed by pasteurization (PS), ultrasound (US), electron beam (EB) irradiation, and high pressure (HP).					
37267648	5	54	theme	Comprehensive	949:961	arg1	analysis					963:970	Comprehensive analysis	949:970	Comprehensive analysis	949:970	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	5	55	dep	related	1023:1029	arg1	reflected					1066:1074	reflected	1066:1074	reflected by the degree of linearity and the integrity of structure	1066:1132	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	5	55	dep	related	1023:1029	arg1	related					1023:1029	related	1023:1029	related	1023:1029	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
37267648	5	55	dep	related	1023:1029	arg1	rate					998:1001	the retention rate	984:1001	the retention rate of ACNs	984:1009	Comprehensive analysis showed that the retention rate of ACNs was closely related to the pectin structure, primarily reflected by the degree of linearity and the integrity of structure.					
35570556	6	0	theme	mv	1150:1151	arg1	nm					1112:1113	402.7±1.3 nm	1102:1113	402.7±1.3 nm	1102:1113	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	6	0	theme	mv	1150:1151	arg1	ZP					1134:1135	ZP	1134:1135	ZP of -24.5±0.2 mv	1134:1151	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	10	1	theme	ciprofloxacin	1614:1626	arg1	nanogels					1638:1645	The ciprofloxacin composite nanogels	1610:1645	The ciprofloxacin composite nanogels	1610:1645	The ciprofloxacin composite nanogels had more excellent antibacterial activity against SCV 102 isolate than S. aureus ATCC 29213 and S. aureus 101 isolates.					
35570556	1	2	theme	bacteria	314:321	arg1	performance					299:309	the antibacterial activity performance	272:309	the antibacterial activity performance of bacteria	272:321	BACKGROUND The development of nanogels has become an attractive strategy to enhance the antibacterial activity performance of bacteria.					
35570556	5	3	link	cross-linked	1034:1045	arg1	networks					1057:1064	nano-sized cross-linked polymeric networks	1023:1064	nano-sized cross-linked polymeric networks	1023:1064	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	2	4	theme	mastitis	653:660	arg1	infection					662:670	Staphylococcus aureus (S. aureus) mastitis infection	619:670	Staphylococcus aureus (S. aureus) mastitis infection	619:670	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	6	5	theme	%	1190:1190	arg1	distributions					1085:1097	The particle size distributions	1067:1097	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.	1067:1191	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	10	6	theme	antibacterial	1666:1678	arg1	activity					1680:1687	more excellent antibacterial activity	1651:1687	more excellent antibacterial activity against SCV 102 isolate	1651:1711	The ciprofloxacin composite nanogels had more excellent antibacterial activity against SCV 102 isolate than S. aureus ATCC 29213 and S. aureus 101 isolates.					
35570556	2	7	theme	composite	350:358	arg1	nanogels					360:367	The ciprofloxacin composite nanogels	332:367	The ciprofloxacin composite nanogels	332:367	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	2	7	theme	composite	350:358	arg1	crosslinkers					541:552	ionic crosslinkers	535:552	ionic crosslinkers	535:552	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	2	8	dep	METHODS	324:330	arg1	prepared					387:394	prepared	387:394	prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers	387:552	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	5	9	theme	homogenous	931:940	arg1	suspension					956:965	homogenous canary yellow suspension	931:965	homogenous canary yellow suspension	931:965	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	5	9	theme	homogenous	931:940	arg1	nanogels					917:924	The optimized ciprofloxacin composite nanogels	879:924	The optimized ciprofloxacin composite nanogels	879:924	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	11	10	theme	nanogels	1859:1866	arg1	promise					1809:1815	the great promise	1799:1815	the great promise of the injectable ciprofloxacin composite nanogels	1799:1866	The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.					
35570556	11	10	theme	nanogels	1859:1866	arg1	injection					1894:1902	a biocompatible breast injection	1871:1902	a biocompatible breast injection	1871:1902	The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.					
35570556	3	11	dep	formulation	677:687	arg1	biosafety					764:772	biosafety	764:772	biosafety	764:772	The formulation screening, characterization, in vitro release, antibacterial activity, and biosafety were studied.					
35570556	3	11	dep	formulation	677:687	arg1	characterization					700:715	characterization	700:715	characterization	700:715	The formulation screening, characterization, in vitro release, antibacterial activity, and biosafety were studied.					
35570556	3	11	dep	formulation	677:687	arg1	screening					689:697	screening	689:697	screening	689:697	The formulation screening, characterization, in vitro release, antibacterial activity, and biosafety were studied.					
35570556	3	11	dep	formulation	677:687	arg1	release					727:733	in vitro release	718:733	in vitro release	718:733	The formulation screening, characterization, in vitro release, antibacterial activity, and biosafety were studied.					
35570556	3	11	dep	formulation	677:687	arg1	activity					750:757	antibacterial activity	736:757	antibacterial activity	736:757	The formulation screening, characterization, in vitro release, antibacterial activity, and biosafety were studied.					
35570556	5	12	theme	sedimentation	974:986	arg1	rate					988:991	a sedimentation rate	972:991	a sedimentation rate of 1	972:996	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	2	13	theme	ciprofloxacin	597:609	arg1	activity					585:592	the antibacterial activity	567:592	the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection	567:670	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	10	14	theme	aureus	1721:1726	arg1	29213					1733:1737	S. aureus ATCC 29213	1718:1737	S. aureus ATCC 29213	1718:1737	The ciprofloxacin composite nanogels had more excellent antibacterial activity against SCV 102 isolate than S. aureus ATCC 29213 and S. aureus 101 isolates.					
35570556	5	15	theme	ciprofloxacin	893:905	arg1	suspension					956:965	homogenous canary yellow suspension	931:965	homogenous canary yellow suspension	931:965	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	5	15	theme	ciprofloxacin	893:905	arg1	nanogels					917:924	The optimized ciprofloxacin composite nanogels	879:924	The optimized ciprofloxacin composite nanogels	879:924	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	9	16	theme	composite	1555:1563	arg1	nanogels					1565:1572	ciprofloxacin composite nanogels	1541:1572	ciprofloxacin composite nanogels	1541:1572	Meanwhile, in vitro release study showed that ciprofloxacin composite nanogels had sustained-release performances.					
35570556	11	17	theme	composite	1849:1857	arg1	nanogels					1859:1866	the injectable ciprofloxacin composite nanogels	1820:1866	the injectable ciprofloxacin composite nanogels	1820:1866	The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.					
35570556	1	18	theme	activity	290:297	arg1	performance					299:309	the antibacterial activity performance	272:309	the antibacterial activity performance of bacteria	272:321	BACKGROUND The development of nanogels has become an attractive strategy to enhance the antibacterial activity performance of bacteria.					
35570556	11	19	theme	injectable	1824:1833	arg1	nanogels					1859:1866	the injectable ciprofloxacin composite nanogels	1820:1866	the injectable ciprofloxacin composite nanogels	1820:1866	The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.					
35570556	5	20	theme	yellow	949:954	arg1	suspension					956:965	homogenous canary yellow suspension	931:965	homogenous canary yellow suspension	931:965	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	5	20	theme	yellow	949:954	arg1	nanogels					917:924	The optimized ciprofloxacin composite nanogels	879:924	The optimized ciprofloxacin composite nanogels	879:924	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	6	21	theme	size	1080:1083	arg1	distributions					1085:1097	The particle size distributions	1067:1097	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.	1067:1191	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	5	22	with	suspension	956:965	arg1	rate					988:991	a sedimentation rate	972:991	a sedimentation rate of 1	972:996	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	8	23	theme	spontaneous	1369:1379	arg1	interactions					1395:1406	the spontaneous electrostatic interactions	1365:1406	the spontaneous electrostatic interactions between CMC and gelatin	1365:1430	Fourier transform infrared showed that the spontaneous electrostatic interactions between CMC and gelatin produce the network structure and form the composite nanogels.					
35570556	11	24	theme	biocompatible	1873:1885	arg1	promise					1809:1815	the great promise	1799:1815	the great promise of the injectable ciprofloxacin composite nanogels	1799:1866	The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.					
35570556	11	24	theme	biocompatible	1873:1885	arg1	injection					1894:1902	a biocompatible breast injection	1871:1902	a biocompatible breast injection	1871:1902	The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.					
35570556	0	25	theme	ciprofloxacin-loaded	78:97	arg1	composite					138:146	injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite	67:146	injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite	67:146	Designing, structural determination, and antibacterial activity of injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite nanogels against Staphylococcus aureus.					
35570556	12	26	theme	aureus	2049:2054	arg1	therapy					2065:2071	cow S. aureus mastitis therapy	2042:2071	cow S. aureus mastitis therapy	2042:2071	CONCLUSION This study will afford a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy.					
35570556	0	27	theme	carboxymethyl	114:126	arg1	composite					138:146	injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite	67:146	injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite	67:146	Designing, structural determination, and antibacterial activity of injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite nanogels against Staphylococcus aureus.					
35570556	5	28	theme	nano-sized	1023:1032	arg1	networks					1057:1064	nano-sized cross-linked polymeric networks	1023:1064	nano-sized cross-linked polymeric networks	1023:1064	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	12	29	dep	CONCLUSION	1905:1914	arg1	afford					1932:1937	afford	1932:1937	will afford a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy	1927:2071	CONCLUSION This study will afford a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy.					
35570556	10	30	theme	aureus	1746:1751	arg1	isolates					1757:1764	S. aureus 101 isolates	1743:1764	S. aureus 101 isolates	1743:1764	The ciprofloxacin composite nanogels had more excellent antibacterial activity against SCV 102 isolate than S. aureus ATCC 29213 and S. aureus 101 isolates.					
35570556	7	31	theme	Scanning	1193:1200	arg1	images					1222:1227	Scanning electron microscope images	1193:1227	Scanning electron microscope images	1193:1227	Scanning electron microscope images revealed that ciprofloxacin might be incorporated in nano-sized cross-linked polymeric networks.					
35570556	5	32	theme	polymeric	1047:1055	arg1	networks					1057:1064	nano-sized cross-linked polymeric networks	1023:1064	nano-sized cross-linked polymeric networks	1023:1064	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	12	33	theme	cow	2042:2044	arg1	therapy					2065:2071	cow S. aureus mastitis therapy	2042:2071	cow S. aureus mastitis therapy	2042:2071	CONCLUSION This study will afford a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy.					
35570556	4	34	dep	RESULTS	788:794	arg1	fabricated					826:835	fabricated	826:835	was fabricated of 20 mg/mL (CMC) and 50 mg/mL (gelatin)	822:876	RESULTS The optimized formulation was fabricated of 20 mg/mL (CMC) and 50 mg/mL (gelatin).					
35570556	6	35	theme	0.12±0.01	1123:1131	arg1	PDI					1116:1118	PDI	1116:1118	PDI of 0.12±0.01	1116:1131	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	6	35	theme	0.12±0.01	1123:1131	arg1	nm					1112:1113	402.7±1.3 nm	1102:1113	402.7±1.3 nm	1102:1113	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	9	36	contain	had	1574:1576	arg1	nanogels					1565:1572	ciprofloxacin composite nanogels	1541:1572	ciprofloxacin composite nanogels	1541:1572	Meanwhile, in vitro release study showed that ciprofloxacin composite nanogels had sustained-release performances.					
35570556	9	36	contain	had	1574:1576	arg2	performances					1596:1607	sustained-release performances	1578:1607	sustained-release performances	1578:1607	Meanwhile, in vitro release study showed that ciprofloxacin composite nanogels had sustained-release performances.					
35570556	1	37	dep	BACKGROUND	188:197	arg1	become					231:236	become	231:236	has become an attractive strategy to enhance the antibacterial activity performance of bacteria	227:321	BACKGROUND The development of nanogels has become an attractive strategy to enhance the antibacterial activity performance of bacteria.					
35570556	0	38	theme	structural	11:20	arg1	determination					22:34	structural determination	11:34	structural determination	11:34	Designing, structural determination, and antibacterial activity of injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite nanogels against Staphylococcus aureus.					
35570556	12	39	theme	injectable	1975:1984	arg1	nanogels					2029:2036	injectable ciprofloxacin-loaded gelatin-CMC composite nanogels	1975:2036	injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy	1975:2071	CONCLUSION This study will afford a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy.					
35570556	2	40	with	interaction	413:423	arg1	the					490:492	the	490:492	the	490:492	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	5	41	theme	canary	942:947	arg1	suspension					956:965	homogenous canary yellow suspension	931:965	homogenous canary yellow suspension	931:965	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	5	41	theme	canary	942:947	arg1	nanogels					917:924	The optimized ciprofloxacin composite nanogels	879:924	The optimized ciprofloxacin composite nanogels	879:924	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	9	42	dep	in	1506:1507	arg1	vitro					1509:1513	vitro	1509:1513	vitro	1509:1513	Meanwhile, in vitro release study showed that ciprofloxacin composite nanogels had sustained-release performances.					
35570556	1	43	theme	nanogels	218:225	arg1	development					203:213	The development	199:213	The development of nanogels	199:225	BACKGROUND The development of nanogels has become an attractive strategy to enhance the antibacterial activity performance of bacteria.					
35570556	12	44	theme	gelatin-CMC	2007:2017	arg1	nanogels					2029:2036	injectable ciprofloxacin-loaded gelatin-CMC composite nanogels	1975:2036	injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy	1975:2071	CONCLUSION This study will afford a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy.					
35570556	6	45	theme	nm	1112:1113	arg1	distributions					1085:1097	The particle size distributions	1067:1097	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.	1067:1191	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	2	46	dep	the	490:492	arg1	help					494:497	help	494:497	help	494:497	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	0	47	theme	antibacterial	41:53	arg1	activity					55:62	antibacterial activity	41:62	antibacterial activity	41:62	Designing, structural determination, and antibacterial activity of injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite nanogels against Staphylococcus aureus.					
35570556	12	48	theme	potential	1941:1949	arg1	approach					1951:1958	a potential approach	1939:1958	a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy	1939:2071	CONCLUSION This study will afford a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy.					
35570556	8	49	theme	composite	1475:1483	arg1	nanogels					1485:1492	the composite nanogels	1471:1492	the composite nanogels	1471:1492	Fourier transform infrared showed that the spontaneous electrostatic interactions between CMC and gelatin produce the network structure and form the composite nanogels.					
35570556	8	50	dep	transform	1334:1342	arg1	infrared					1344:1351	infrared	1344:1351	transform infrared	1334:1351	Fourier transform infrared showed that the spontaneous electrostatic interactions between CMC and gelatin produce the network structure and form the composite nanogels.					
35570556	7	51	theme	cross-linked	1293:1304	arg1	networks					1316:1323	nano-sized cross-linked polymeric networks	1282:1323	nano-sized cross-linked polymeric networks	1282:1323	Scanning electron microscope images revealed that ciprofloxacin might be incorporated in nano-sized cross-linked polymeric networks.					
35570556	3	52	dep	in	718:719	arg1	vitro					721:725	vitro	721:725	vitro	721:725	The formulation screening, characterization, in vitro release, antibacterial activity, and biosafety were studied.					
35570556	2	53	dep	aureus	634:639	arg1	aureus					645:650	S. aureus	642:650	S. aureus	642:650	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	11	54	theme	ciprofloxacin	1835:1847	arg1	nanogels					1859:1866	the injectable ciprofloxacin composite nanogels	1820:1866	the injectable ciprofloxacin composite nanogels	1820:1866	The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.					
35570556	10	55	theme	composite	1628:1636	arg1	nanogels					1638:1645	The ciprofloxacin composite nanogels	1610:1645	The ciprofloxacin composite nanogels	1610:1645	The ciprofloxacin composite nanogels had more excellent antibacterial activity against SCV 102 isolate than S. aureus ATCC 29213 and S. aureus 101 isolates.					
35570556	2	56	theme	positive	442:449	arg1	gelatin					433:439	gelatin	433:439	gelatin (positive charge)	433:457	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	2	56	theme	positive	442:449	arg1	charge					451:456	positive charge	442:456	positive charge	442:456	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	6	57	theme	±0.05	1185:1189	arg1	%					1184:1184	%	1184:1184	%	1184:1184	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	10	58	theme	excellent	1656:1664	arg1	activity					1680:1687	more excellent antibacterial activity	1651:1687	more excellent antibacterial activity against SCV 102 isolate	1651:1711	The ciprofloxacin composite nanogels had more excellent antibacterial activity against SCV 102 isolate than S. aureus ATCC 29213 and S. aureus 101 isolates.					
35570556	6	59	theme	±0.03	1166:1170	arg1	%					1165:1165	%	1165:1165	%	1165:1165	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	2	60	theme	ciprofloxacin	336:348	arg1	nanogels					360:367	The ciprofloxacin composite nanogels	332:367	The ciprofloxacin composite nanogels	332:367	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	2	60	theme	ciprofloxacin	336:348	arg1	crosslinkers					541:552	ionic crosslinkers	535:552	ionic crosslinkers	535:552	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	5	61	theme	composite	907:915	arg1	suspension					956:965	homogenous canary yellow suspension	931:965	homogenous canary yellow suspension	931:965	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	5	61	theme	composite	907:915	arg1	nanogels					917:924	The optimized ciprofloxacin composite nanogels	879:924	The optimized ciprofloxacin composite nanogels	879:924	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	6	62	theme	20.5	1180:1183	arg1	%					1184:1184	%	1184:1184	%	1184:1184	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	9	63	theme	in	1506:1507	arg1	study					1523:1527	in vitro release study	1506:1527	in vitro release study	1506:1527	Meanwhile, in vitro release study showed that ciprofloxacin composite nanogels had sustained-release performances.					
35570556	9	63	theme	in	1506:1507	arg1	Meanwhile					1495:1503	Meanwhile	1495:1503	Meanwhile	1495:1503	Meanwhile, in vitro release study showed that ciprofloxacin composite nanogels had sustained-release performances.					
35570556	10	64	theme	SCV	1697:1699	arg1	isolate					1705:1711	SCV 102 isolate	1697:1711	SCV 102 isolate	1697:1711	The ciprofloxacin composite nanogels had more excellent antibacterial activity against SCV 102 isolate than S. aureus ATCC 29213 and S. aureus 101 isolates.					
35570556	9	65	theme	release	1515:1521	arg1	study					1523:1527	in vitro release study	1506:1527	in vitro release study	1506:1527	Meanwhile, in vitro release study showed that ciprofloxacin composite nanogels had sustained-release performances.					
35570556	9	65	theme	release	1515:1521	arg1	Meanwhile					1495:1503	Meanwhile	1495:1503	Meanwhile	1495:1503	Meanwhile, in vitro release study showed that ciprofloxacin composite nanogels had sustained-release performances.					
35570556	6	66	theme	%	1171:1171	arg1	nm					1112:1113	402.7±1.3 nm	1102:1113	402.7±1.3 nm	1102:1113	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	6	66	theme	%	1171:1171	arg1	EE					1154:1155	EE	1154:1155	EE of 74.28%±0.03%	1154:1171	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	10	67	theme	ATCC	1728:1731	arg1	29213					1733:1737	S. aureus ATCC 29213	1718:1737	S. aureus ATCC 29213	1718:1737	The ciprofloxacin composite nanogels had more excellent antibacterial activity against SCV 102 isolate than S. aureus ATCC 29213 and S. aureus 101 isolates.					
35570556	5	68	theme	optimized	883:891	arg1	suspension					956:965	homogenous canary yellow suspension	931:965	homogenous canary yellow suspension	931:965	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	5	68	theme	optimized	883:891	arg1	nanogels					917:924	The optimized ciprofloxacin composite nanogels	879:924	The optimized ciprofloxacin composite nanogels	879:924	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	10	69	theme	S.	1718:1719	arg1	29213					1733:1737	S. aureus ATCC 29213	1718:1737	S. aureus ATCC 29213	1718:1737	The ciprofloxacin composite nanogels had more excellent antibacterial activity against SCV 102 isolate than S. aureus ATCC 29213 and S. aureus 101 isolates.					
35570556	7	70	theme	electron	1202:1209	arg1	images					1222:1227	Scanning electron microscope images	1193:1227	Scanning electron microscope images	1193:1227	Scanning electron microscope images revealed that ciprofloxacin might be incorporated in nano-sized cross-linked polymeric networks.					
35570556	9	71	theme	ciprofloxacin	1541:1553	arg1	nanogels					1565:1572	ciprofloxacin composite nanogels	1541:1572	ciprofloxacin composite nanogels	1541:1572	Meanwhile, in vitro release study showed that ciprofloxacin composite nanogels had sustained-release performances.					
35570556	7	72	link	cross-linked	1293:1304	arg1	networks					1316:1323	nano-sized cross-linked polymeric networks	1282:1323	nano-sized cross-linked polymeric networks	1282:1323	Scanning electron microscope images revealed that ciprofloxacin might be incorporated in nano-sized cross-linked polymeric networks.					
35570556	2	73	theme	aureus	634:639	arg1	infection					662:670	Staphylococcus aureus (S. aureus) mastitis infection	619:670	Staphylococcus aureus (S. aureus) mastitis infection	619:670	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	7	74	theme	nano-sized	1282:1291	arg1	networks					1316:1323	nano-sized cross-linked polymeric networks	1282:1323	nano-sized cross-linked polymeric networks	1282:1323	Scanning electron microscope images revealed that ciprofloxacin might be incorporated in nano-sized cross-linked polymeric networks.					
35570556	0	75	theme	injectable	67:76	arg1	composite					138:146	injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite	67:146	injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite	67:146	Designing, structural determination, and antibacterial activity of injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite nanogels against Staphylococcus aureus.					
35570556	1	76	theme	attractive	241:250	arg1	strategy					252:259	an attractive strategy	238:259	an attractive strategy to enhance the antibacterial activity performance of bacteria	238:321	BACKGROUND The development of nanogels has become an attractive strategy to enhance the antibacterial activity performance of bacteria.					
35570556	9	77	theme	sustained-release	1578:1594	arg1	performances					1596:1607	sustained-release performances	1578:1607	sustained-release performances	1578:1607	Meanwhile, in vitro release study showed that ciprofloxacin composite nanogels had sustained-release performances.					
35570556	7	78	theme	microscope	1211:1220	arg1	images					1222:1227	Scanning electron microscope images	1193:1227	Scanning electron microscope images	1193:1227	Scanning electron microscope images revealed that ciprofloxacin might be incorporated in nano-sized cross-linked polymeric networks.					
35570556	0	79	theme	gelatin-sodium	99:112	arg1	composite					138:146	injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite	67:146	injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite	67:146	Designing, structural determination, and antibacterial activity of injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite nanogels against Staphylococcus aureus.					
35570556	12	80	theme	mastitis	2056:2063	arg1	therapy					2065:2071	cow S. aureus mastitis therapy	2042:2071	cow S. aureus mastitis therapy	2042:2071	CONCLUSION This study will afford a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy.					
35570556	6	81	theme	particle	1071:1078	arg1	distributions					1085:1097	The particle size distributions	1067:1097	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.	1067:1191	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	2	82	theme	ionic	535:539	arg1	nanogels					360:367	The ciprofloxacin composite nanogels	332:367	The ciprofloxacin composite nanogels	332:367	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	2	82	theme	ionic	535:539	arg1	crosslinkers					541:552	ionic crosslinkers	535:552	ionic crosslinkers	535:552	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	11	83	theme	breast	1887:1892	arg1	promise					1809:1815	the great promise	1799:1815	the great promise of the injectable ciprofloxacin composite nanogels	1799:1866	The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.					
35570556	11	83	theme	breast	1887:1892	arg1	injection					1894:1902	a biocompatible breast injection	1871:1902	a biocompatible breast injection	1871:1902	The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.					
35570556	0	84	theme	cellulose	128:136	arg1	composite					138:146	injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite	67:146	injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite	67:146	Designing, structural determination, and antibacterial activity of injectable ciprofloxacin-loaded gelatin-sodium carboxymethyl cellulose composite nanogels against Staphylococcus aureus.					
35570556	2	85	theme	antibacterial	571:583	arg1	activity					585:592	the antibacterial activity	567:592	the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection	567:670	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	8	86	theme	electrostatic	1381:1393	arg1	interactions					1395:1406	the spontaneous electrostatic interactions	1365:1406	the spontaneous electrostatic interactions between CMC and gelatin	1365:1430	Fourier transform infrared showed that the spontaneous electrostatic interactions between CMC and gelatin produce the network structure and form the composite nanogels.					
35570556	5	87	theme	cross-linked	1034:1045	arg1	networks					1057:1064	nano-sized cross-linked polymeric networks	1023:1064	nano-sized cross-linked polymeric networks	1023:1064	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	12	88	theme	S.	2046:2047	arg1	therapy					2065:2071	cow S. aureus mastitis therapy	2042:2071	cow S. aureus mastitis therapy	2042:2071	CONCLUSION This study will afford a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy.					
35570556	5	89	theme	1	996:996	arg1	rate					988:991	a sedimentation rate	972:991	a sedimentation rate of 1	972:996	The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks.					
35570556	2	90	theme	sodium	502:507	arg1	TPP					527:529	TPP	527:529	TPP	527:529	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	2	90	theme	sodium	502:507	arg1	tripolyphosphate					509:524	sodium tripolyphosphate	502:524	sodium tripolyphosphate (TPP)	502:530	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	4	91	theme	optimized	800:808	arg1	formulation					810:820	The optimized formulation	796:820	The optimized formulation	796:820	RESULTS The optimized formulation was fabricated of 20 mg/mL (CMC) and 50 mg/mL (gelatin).					
35570556	12	92	theme	ciprofloxacin-loaded	1986:2005	arg1	nanogels					2029:2036	injectable ciprofloxacin-loaded gelatin-CMC composite nanogels	1975:2036	injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy	1975:2071	CONCLUSION This study will afford a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy.					
35570556	3	93	theme	in	718:719	arg1	release					727:733	in vitro release	718:733	in vitro release	718:733	The formulation screening, characterization, in vitro release, antibacterial activity, and biosafety were studied.					
35570556	12	94	theme	composite	2019:2027	arg1	nanogels					2029:2036	injectable ciprofloxacin-loaded gelatin-CMC composite nanogels	1975:2036	injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy	1975:2071	CONCLUSION This study will afford a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy.					
35570556	2	95	theme	negative	468:475	arg1	CMC					463:465	CMC	463:465	CMC (negative charge)	463:483	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	2	95	theme	negative	468:475	arg1	charge					477:482	negative charge	468:482	negative charge	468:482	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	11	96	theme	biosafety	1771:1779	arg1	studies					1781:1787	The biosafety studies	1767:1787	The biosafety studies	1767:1787	The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.					
35570556	8	97	theme	network	1444:1450	arg1	structure					1452:1460	the network structure	1440:1460	the network structure	1440:1460	Fourier transform infrared showed that the spontaneous electrostatic interactions between CMC and gelatin produce the network structure and form the composite nanogels.					
35570556	7	98	theme	polymeric	1306:1314	arg1	networks					1316:1323	nano-sized cross-linked polymeric networks	1282:1323	nano-sized cross-linked polymeric networks	1282:1323	Scanning electron microscope images revealed that ciprofloxacin might be incorporated in nano-sized cross-linked polymeric networks.					
35570556	3	99	theme	antibacterial	736:748	arg1	activity					750:757	antibacterial activity	736:757	antibacterial activity	736:757	The formulation screening, characterization, in vitro release, antibacterial activity, and biosafety were studied.					
35570556	6	100	theme	74.28	1160:1164	arg1	%					1165:1165	%	1165:1165	%	1165:1165	The particle size distributions of 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2 mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%.					
35570556	10	101	contain	had	1647:1649	arg1	nanogels					1638:1645	The ciprofloxacin composite nanogels	1610:1645	The ciprofloxacin composite nanogels	1610:1645	The ciprofloxacin composite nanogels had more excellent antibacterial activity against SCV 102 isolate than S. aureus ATCC 29213 and S. aureus 101 isolates.					
35570556	10	101	contain	had	1647:1649	arg2	activity					1680:1687	more excellent antibacterial activity	1651:1687	more excellent antibacterial activity against SCV 102 isolate	1651:1711	The ciprofloxacin composite nanogels had more excellent antibacterial activity against SCV 102 isolate than S. aureus ATCC 29213 and S. aureus 101 isolates.					
35570556	11	102	theme	great	1803:1807	arg1	promise					1809:1815	the great promise	1799:1815	the great promise of the injectable ciprofloxacin composite nanogels	1799:1866	The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.					
35570556	11	102	theme	great	1803:1807	arg1	injection					1894:1902	a biocompatible breast injection	1871:1902	a biocompatible breast injection	1871:1902	The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.					
35570556	2	103	theme	electrostatic	399:411	arg1	interaction					413:423	electrostatic interaction	399:423	electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP)	399:530	METHODS The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection.					
35570556	1	104	theme	antibacterial	276:288	arg1	performance					299:309	the antibacterial activity performance	272:309	the antibacterial activity performance of bacteria	272:321	BACKGROUND The development of nanogels has become an attractive strategy to enhance the antibacterial activity performance of bacteria.					
35570556	8	105	dep	Fourier	1326:1332	arg1	transform					1334:1342	transform	1334:1342	transform infrared	1334:1351	Fourier transform infrared showed that the spontaneous electrostatic interactions between CMC and gelatin produce the network structure and form the composite nanogels.					
36179472	3	0	theme	visible	718:724	arg1	irradiation					732:742	visible light irradiation	718:742	visible light irradiation	718:742	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	6	1	from	study	1127:1131	arg1	%					1205:1205	38.7%	1201:1205	38.7%	1201:1205	From the kinetic study, the effect of mixing two composites, i.e., synergy percentage, was 38.7% when ZnO-MgFe2O4 particles were added to the GO-pectin structure.					
36179472	6	1	from	study	1127:1131	arg1	effect					1138:1143	the effect	1134:1143	the effect of mixing two composites, i.e., synergy percentage,	1134:1195	From the kinetic study, the effect of mixing two composites, i.e., synergy percentage, was 38.7% when ZnO-MgFe2O4 particles were added to the GO-pectin structure.					
36179472	6	2	theme	kinetic	1119:1125	arg1	study					1127:1131	the kinetic study	1115:1131	the kinetic study	1115:1131	From the kinetic study, the effect of mixing two composites, i.e., synergy percentage, was 38.7% when ZnO-MgFe2O4 particles were added to the GO-pectin structure.					
36179472	0	3	theme	photocatalytic	105:118	arg1	degradation					120:130	photocatalytic degradation	105:130	photocatalytic degradation of diclofenac in an aqueous solution	105:167	Fabrication and characterization of pectin-graphene oxide-magnesium ferrite-zinc oxide nanocomposite for photocatalytic degradation of diclofenac in an aqueous solution under visible light irradiation.					
36179472	3	4	theme	aquatic	692:698	arg1	environment					700:710	an aquatic environment	689:710	an aquatic environment under visible light irradiation	689:742	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	5	5	theme	degradation	1085:1095	arg1	performance					1097:1107	the degradation performance	1081:1107	the degradation performance	1081:1107	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	3	6	theme	novel	549:553	arg1	nanocomposite					627:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite	547:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF	547:684	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	3	6	theme	novel	549:553	arg1	PGMZ					642:645	PGMZ	642:645	PGMZ	642:645	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	3	7	theme	photocatalytic	652:665	arg1	degradation					667:677	photocatalytic degradation	652:677	photocatalytic degradation of DCF	652:684	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	0	8	theme	diclofenac	135:144	arg1	degradation					120:130	photocatalytic degradation	105:130	photocatalytic degradation of diclofenac in an aqueous solution	105:167	Fabrication and characterization of pectin-graphene oxide-magnesium ferrite-zinc oxide nanocomposite for photocatalytic degradation of diclofenac in an aqueous solution under visible light irradiation.					
36179472	8	9	theme	removal	1650:1656	arg1	efficiency					1658:1667	its removal efficiency	1646:1667	its removal efficiency	1646:1667	Finally, the PGMZ photocatalyst was reused six times with a reduction of about 20% in its removal efficiency, indicating excellent reusability and stability.					
36179472	5	10	theme	nanocomposite	933:945	arg1	dosage					947:952	nanocomposite dosage	933:952	nanocomposite dosage (1-1.25 g/L)	933:965	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	5	10	theme	nanocomposite	933:945	arg1	1-1.25 g/L					955:964	1-1.25 g/L	955:964	1-1.25 g/L	955:964	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	5	11	theme	contaminant	996:1006	arg1	35-55 mg/L					1023:1032	35-55 mg/L	1023:1032	35-55 mg/L	1023:1032	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	5	11	theme	contaminant	996:1006	arg1	concentration					1008:1020	initial contaminant concentration	988:1020	initial contaminant concentration (35-55 mg/L)	988:1033	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	1	12	theme	drug	325:328	arg1	consumption					330:340	drug consumption	325:340	drug consumption	325:340	Wastewater containing pharmaceutical contaminants has become a critical environmental concern due to rising population and drug consumption caused by increased life expectancy.					
36179472	7	13	theme	optimal	1537:1543	arg1	concentration					1545:1557	their optimal concentration	1531:1557	their optimal concentration	1531:1557	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	3	14	theme	-magnesium	581:590	arg1	nanocomposite					627:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite	547:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF	547:684	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	3	14	theme	-magnesium	581:590	arg1	PGMZ					642:645	PGMZ	642:645	PGMZ	642:645	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	7	15	dep	order	1452:1456	arg1	NaCl > Na2CO3 > Na2SO4					1459:1480	NaCl > Na2CO3 > Na2SO4	1459:1480	NaCl > Na2CO3 > Na2SO4	1459:1480	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	7	16	from	concentration	1545:1557	arg1	H2O2					1523:1526	H2O2	1523:1526	H2O2 at their optimal concentration	1523:1557	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	6	17	theme	GO-pectin	1252:1260	arg1	structure					1262:1270	the GO-pectin structure	1248:1270	the GO-pectin structure	1248:1270	From the kinetic study, the effect of mixing two composites, i.e., synergy percentage, was 38.7% when ZnO-MgFe2O4 particles were added to the GO-pectin structure.					
36179472	3	18	dep	nanocomposite	627:639	arg1	GO					578:579	GO	578:579	GO	578:579	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	4	19	theme	several	809:815	arg1	analyses					817:824	several analyses	809:824	several analyses	809:824	The single and synthesized nanocomposites were characterized by several analyses, confirming the successful synthesis of the nanocomposite.					
36179472	7	20	theme	radical	1315:1321	arg1	enhancers					1323:1331	different free radical enhancers	1300:1331	different free radical enhancers	1300:1331	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	2	21	theme	different	502:510	arg1	ways					512:515	different ways	502:515	different ways	502:515	Diclofenac (DCF) is one of the most applicable drugs for veterinary and human health purposes, polluting surface waters in different ways.					
36179472	3	22	theme	light	726:730	arg1	irradiation					732:742	visible light irradiation	718:742	visible light irradiation	718:742	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	4	23	theme	nanocomposite	870:882	arg1	synthesis					853:861	the successful synthesis	838:861	the successful synthesis of the nanocomposite	838:882	The single and synthesized nanocomposites were characterized by several analyses, confirming the successful synthesis of the nanocomposite.					
36179472	6	24	theme	composites	1159:1168	arg1	%					1205:1205	38.7%	1201:1205	38.7%	1201:1205	From the kinetic study, the effect of mixing two composites, i.e., synergy percentage, was 38.7% when ZnO-MgFe2O4 particles were added to the GO-pectin structure.					
36179472	6	24	theme	composites	1159:1168	arg1	effect					1138:1143	the effect	1134:1143	the effect of mixing two composites, i.e., synergy percentage,	1134:1195	From the kinetic study, the effect of mixing two composites, i.e., synergy percentage, was 38.7% when ZnO-MgFe2O4 particles were added to the GO-pectin structure.					
36179472	8	25	with	times	1607:1611	arg1	reduction					1620:1628	a reduction	1618:1628	a reduction of about 20% in its removal efficiency	1618:1667	Finally, the PGMZ photocatalyst was reused six times with a reduction of about 20% in its removal efficiency, indicating excellent reusability and stability.					
36179472	1	26	theme	pharmaceutical	224:237	arg1	contaminants					239:250	pharmaceutical contaminants	224:250	pharmaceutical contaminants	224:250	Wastewater containing pharmaceutical contaminants has become a critical environmental concern due to rising population and drug consumption caused by increased life expectancy.					
36179472	3	27	theme	MgFe2O4	601:607	arg1	nanocomposite					627:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite	547:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF	547:684	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	3	27	theme	MgFe2O4	601:607	arg1	PGMZ					642:645	PGMZ	642:645	PGMZ	642:645	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	5	28	theme	conditions	911:920	arg1	Effects					885:891	Effects	885:891	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11),	885:1058	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	7	29	theme	following	1442:1450	arg1	order					1452:1456	the following order; NaCl > Na2CO3 > Na2SO4	1438:1480	the following order; NaCl > Na2CO3 > Na2SO4	1438:1480	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	3	30	theme	DCF	682:684	arg1	degradation					667:677	photocatalytic degradation	652:677	photocatalytic degradation of DCF	652:684	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	7	31	theme	better	1502:1507	arg1	enhancer					1509:1516	a better enhancer	1500:1516	a better enhancer	1500:1516	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	7	31	theme	better	1502:1507	arg1	K2S2O8					1489:1494	K2S2O8	1489:1494	K2S2O8	1489:1494	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	4	32	theme	single	749:754	arg1	nanocomposites					772:785	The single and synthesized nanocomposites	745:785	The single and synthesized nanocomposites	745:785	The single and synthesized nanocomposites were characterized by several analyses, confirming the successful synthesis of the nanocomposite.					
36179472	8	33	from	reduction	1620:1628	arg1	efficiency					1658:1667	its removal efficiency	1646:1667	its removal efficiency	1646:1667	Finally, the PGMZ photocatalyst was reused six times with a reduction of about 20% in its removal efficiency, indicating excellent reusability and stability.					
36179472	2	34	theme	drugs	426:430	arg1	drugs					426:430	the most applicable drugs	406:430	the most applicable drugs for veterinary and human health purposes	406:471	Diclofenac (DCF) is one of the most applicable drugs for veterinary and human health purposes, polluting surface waters in different ways.					
36179472	2	34	theme	drugs	426:430	arg1	one					399:401	one	399:401	one	399:401	Diclofenac (DCF) is one of the most applicable drugs for veterinary and human health purposes, polluting surface waters in different ways.					
36179472	2	35	theme	surface	484:490	arg1	waters					492:497	surface waters	484:497	surface waters	484:497	Diclofenac (DCF) is one of the most applicable drugs for veterinary and human health purposes, polluting surface waters in different ways.					
36179472	7	36	theme	compounds	1348:1356	arg1	effect					1290:1295	the effect	1286:1295	the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation	1286:1384	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	6	37	dep	percentage	1185:1194	arg1	i.e.					1171:1174	i.e.	1171:1174	i.e.	1171:1174	From the kinetic study, the effect of mixing two composites, i.e., synergy percentage, was 38.7% when ZnO-MgFe2O4 particles were added to the GO-pectin structure.					
36179472	6	38	theme	ZnO-MgFe2O4	1212:1222	arg1	particles					1224:1232	ZnO-MgFe2O4 particles	1212:1232	ZnO-MgFe2O4 particles	1212:1232	From the kinetic study, the effect of mixing two composites, i.e., synergy percentage, was 38.7% when ZnO-MgFe2O4 particles were added to the GO-pectin structure.					
36179472	3	39	theme	ferrite	592:598	arg1	nanocomposite					627:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite	547:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF	547:684	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	3	39	theme	ferrite	592:598	arg1	PGMZ					642:645	PGMZ	642:645	PGMZ	642:645	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	7	40	theme	different	1300:1308	arg1	enhancers					1323:1331	different free radical enhancers	1300:1331	different free radical enhancers	1300:1331	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	1	41	theme	increased	352:360	arg1	expectancy					367:376	increased life expectancy	352:376	increased life expectancy	352:376	Wastewater containing pharmaceutical contaminants has become a critical environmental concern due to rising population and drug consumption caused by increased life expectancy.					
36179472	0	42	theme	aqueous	152:158	arg1	solution					160:167	an aqueous solution	149:167	an aqueous solution	149:167	Fabrication and characterization of pectin-graphene oxide-magnesium ferrite-zinc oxide nanocomposite for photocatalytic degradation of diclofenac in an aqueous solution under visible light irradiation.					
36179472	0	43	from	degradation	120:130	arg1	solution					160:167	an aqueous solution	149:167	an aqueous solution	149:167	Fabrication and characterization of pectin-graphene oxide-magnesium ferrite-zinc oxide nanocomposite for photocatalytic degradation of diclofenac in an aqueous solution under visible light irradiation.					
36179472	4	44	theme	synthesized	760:770	arg1	nanocomposites					772:785	The single and synthesized nanocomposites	745:785	The single and synthesized nanocomposites	745:785	The single and synthesized nanocomposites were characterized by several analyses, confirming the successful synthesis of the nanocomposite.					
36179472	8	45	theme	PGMZ	1573:1576	arg1	times					1607:1611	six times	1603:1611	reused six times	1596:1611	Finally, the PGMZ photocatalyst was reused six times with a reduction of about 20% in its removal efficiency, indicating excellent reusability and stability.					
36179472	8	45	theme	PGMZ	1573:1576	arg1	photocatalyst					1578:1590	the PGMZ photocatalyst	1569:1590	the PGMZ photocatalyst	1569:1590	Finally, the PGMZ photocatalyst was reused six times with a reduction of about 20% in its removal efficiency, indicating excellent reusability and stability.					
36179472	3	46	theme	pectin-graphene	555:569	arg1	nanocomposite					627:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite	547:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF	547:684	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	3	46	theme	pectin-graphene	555:569	arg1	PGMZ					642:645	PGMZ	642:645	PGMZ	642:645	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	0	47	theme	oxide-magnesium	52:66	arg1	oxide					81:85	pectin-graphene oxide-magnesium ferrite-zinc oxide	36:85	pectin-graphene oxide-magnesium ferrite-zinc oxide	36:85	Fabrication and characterization of pectin-graphene oxide-magnesium ferrite-zinc oxide nanocomposite for photocatalytic degradation of diclofenac in an aqueous solution under visible light irradiation.					
36179472	1	48	theme	life	362:365	arg1	expectancy					367:376	increased life expectancy	352:376	increased life expectancy	352:376	Wastewater containing pharmaceutical contaminants has become a critical environmental concern due to rising population and drug consumption caused by increased life expectancy.					
36179472	0	49	theme	visible	175:181	arg1	irradiation					189:199	visible light irradiation	175:199	visible light irradiation	175:199	Fabrication and characterization of pectin-graphene oxide-magnesium ferrite-zinc oxide nanocomposite for photocatalytic degradation of diclofenac in an aqueous solution under visible light irradiation.					
36179472	1	50	theme	critical	265:272	arg1	concern					288:294	a critical environmental concern	263:294	a critical environmental concern due to rising population and drug consumption caused by increased life expectancy	263:376	Wastewater containing pharmaceutical contaminants has become a critical environmental concern due to rising population and drug consumption caused by increased life expectancy.					
36179472	7	51	theme	enhancers	1323:1331	arg1	effect					1290:1295	the effect	1286:1295	the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation	1286:1384	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	2	52	theme	health	457:462	arg1	purposes					464:471	veterinary and human health purposes	436:471	veterinary and human health purposes	436:471	Diclofenac (DCF) is one of the most applicable drugs for veterinary and human health purposes, polluting surface waters in different ways.					
36179472	0	53	theme	pectin-graphene	36:50	arg1	oxide					81:85	pectin-graphene oxide-magnesium ferrite-zinc oxide	36:85	pectin-graphene oxide-magnesium ferrite-zinc oxide	36:85	Fabrication and characterization of pectin-graphene oxide-magnesium ferrite-zinc oxide nanocomposite for photocatalytic degradation of diclofenac in an aqueous solution under visible light irradiation.					
36179472	5	54	theme	operation	901:909	arg1	dosage					947:952	nanocomposite dosage	933:952	nanocomposite dosage (1-1.25 g/L)	933:965	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	5	54	theme	operation	901:909	arg1	type					982:985	nanocomposite type	968:985	nanocomposite type	968:985	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	5	54	theme	operation	901:909	arg1	pH					1049:1050	solution pH	1040:1050	solution pH (3-11)	1040:1057	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	5	54	theme	operation	901:909	arg1	conditions					911:920	four operation conditions	896:920	four operation conditions	896:920	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	5	54	theme	operation	901:909	arg1	concentration					1008:1020	initial contaminant concentration	988:1020	initial contaminant concentration (35-55 mg/L)	988:1033	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	7	55	theme	influential	1396:1406	arg1	components					1419:1428	the most influential scavenging components	1387:1428	the most influential scavenging components	1387:1428	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	3	56	theme	ZnO	622:624	arg1	nanocomposite					627:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite	547:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF	547:684	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	3	56	theme	ZnO	622:624	arg1	PGMZ					642:645	PGMZ	642:645	PGMZ	642:645	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	7	57	from	effect	1290:1295	arg1	photodegradation					1369:1384	the DCF photodegradation	1361:1384	the DCF photodegradation	1361:1384	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	1	58	theme	environmental	274:286	arg1	concern					288:294	a critical environmental concern	263:294	a critical environmental concern due to rising population and drug consumption caused by increased life expectancy	263:376	Wastewater containing pharmaceutical contaminants has become a critical environmental concern due to rising population and drug consumption caused by increased life expectancy.					
36179472	0	59	theme	oxide	81:85	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of pectin-graphene oxide-magnesium ferrite-zinc oxide nanocomposite for photocatalytic degradation of diclofenac in an aqueous solution under visible light irradiation.					
36179472	0	59	theme	oxide	81:85	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of pectin-graphene oxide-magnesium ferrite-zinc oxide nanocomposite for photocatalytic degradation of diclofenac in an aqueous solution under visible light irradiation.					
36179472	1	60	contain	containing	213:222	arg2	contaminants					239:250	pharmaceutical contaminants	224:250	pharmaceutical contaminants	224:250	Wastewater containing pharmaceutical contaminants has become a critical environmental concern due to rising population and drug consumption caused by increased life expectancy.					
36179472	1	60	contain	containing	213:222	arg1	Wastewater					202:211	Wastewater	202:211	Wastewater containing pharmaceutical contaminants	202:250	Wastewater containing pharmaceutical contaminants has become a critical environmental concern due to rising population and drug consumption caused by increased life expectancy.					
36179472	3	61	theme	-zinc	609:613	arg1	nanocomposite					627:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite	547:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF	547:684	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	3	61	theme	-zinc	609:613	arg1	PGMZ					642:645	PGMZ	642:645	PGMZ	642:645	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	6	62	theme	mixing	1148:1153	arg1	composites					1159:1168	mixing two composites	1148:1168	mixing two composites	1148:1168	From the kinetic study, the effect of mixing two composites, i.e., synergy percentage, was 38.7% when ZnO-MgFe2O4 particles were added to the GO-pectin structure.					
36179472	0	63	theme	ferrite-zinc	68:79	arg1	oxide					81:85	pectin-graphene oxide-magnesium ferrite-zinc oxide	36:85	pectin-graphene oxide-magnesium ferrite-zinc oxide	36:85	Fabrication and characterization of pectin-graphene oxide-magnesium ferrite-zinc oxide nanocomposite for photocatalytic degradation of diclofenac in an aqueous solution under visible light irradiation.					
36179472	0	64	theme	light	183:187	arg1	irradiation					189:199	visible light irradiation	175:199	visible light irradiation	175:199	Fabrication and characterization of pectin-graphene oxide-magnesium ferrite-zinc oxide nanocomposite for photocatalytic degradation of diclofenac in an aqueous solution under visible light irradiation.					
36179472	3	65	theme	oxide	615:619	arg1	nanocomposite					627:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite	547:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF	547:684	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	3	65	theme	oxide	615:619	arg1	PGMZ					642:645	PGMZ	642:645	PGMZ	642:645	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	7	66	theme	scavenging	1337:1346	arg1	compounds					1348:1356	scavenging compounds	1337:1356	scavenging compounds	1337:1356	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	7	67	theme	free	1310:1313	arg1	enhancers					1323:1331	different free radical enhancers	1300:1331	different free radical enhancers	1300:1331	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	8	68	theme	reused	1596:1601	arg1	times					1607:1611	six times	1603:1611	reused six times	1596:1611	Finally, the PGMZ photocatalyst was reused six times with a reduction of about 20% in its removal efficiency, indicating excellent reusability and stability.					
36179472	8	68	theme	reused	1596:1601	arg1	photocatalyst					1578:1590	the PGMZ photocatalyst	1569:1590	the PGMZ photocatalyst	1569:1590	Finally, the PGMZ photocatalyst was reused six times with a reduction of about 20% in its removal efficiency, indicating excellent reusability and stability.					
36179472	6	69	theme	synergy	1177:1183	arg1	percentage					1185:1194	synergy percentage	1177:1194	synergy percentage	1177:1194	From the kinetic study, the effect of mixing two composites, i.e., synergy percentage, was 38.7% when ZnO-MgFe2O4 particles were added to the GO-pectin structure.					
36179472	4	70	theme	successful	842:851	arg1	synthesis					853:861	the successful synthesis	838:861	the successful synthesis of the nanocomposite	838:882	The single and synthesized nanocomposites were characterized by several analyses, confirming the successful synthesis of the nanocomposite.					
36179472	5	71	theme	initial	988:994	arg1	35-55 mg/L					1023:1032	35-55 mg/L	1023:1032	35-55 mg/L	1023:1032	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	5	71	theme	initial	988:994	arg1	concentration					1008:1020	initial contaminant concentration	988:1020	initial contaminant concentration (35-55 mg/L)	988:1033	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	7	72	theme	scavenging	1408:1417	arg1	components					1419:1428	the most influential scavenging components	1387:1428	the most influential scavenging components	1387:1428	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	7	73	theme	DCF	1365:1367	arg1	photodegradation					1369:1384	the DCF photodegradation	1361:1384	the DCF photodegradation	1361:1384	By examining the effect of different free radical enhancers and scavenging compounds on the DCF photodegradation, the most influential scavenging components were in the following order; NaCl > Na2CO3 > Na2SO4, while K2S2O8 was a better enhancer than H2O2 at their optimal concentration.					
36179472	5	74	theme	nanocomposite	968:980	arg1	type					982:985	nanocomposite type	968:985	nanocomposite type	968:985	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	1	75	theme	due	296:298	arg1	concern					288:294	a critical environmental concern	263:294	a critical environmental concern due to rising population and drug consumption caused by increased life expectancy	263:376	Wastewater containing pharmaceutical contaminants has become a critical environmental concern due to rising population and drug consumption caused by increased life expectancy.					
36179472	2	76	theme	human	451:455	arg1	purposes					464:471	veterinary and human health purposes	436:471	veterinary and human health purposes	436:471	Diclofenac (DCF) is one of the most applicable drugs for veterinary and human health purposes, polluting surface waters in different ways.					
36179472	5	77	theme	solution	1040:1047	arg1	3-11					1053:1056	3-11	1053:1056	3-11	1053:1056	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	5	77	theme	solution	1040:1047	arg1	pH					1049:1050	solution pH	1040:1050	solution pH (3-11)	1040:1057	Effects of four operation conditions, including nanocomposite dosage (1-1.25 g/L), nanocomposite type, initial contaminant concentration (35-55 mg/L), and solution pH (3-11), were investigated on the degradation performance.					
36179472	2	78	theme	applicable	415:424	arg1	drugs					426:430	the most applicable drugs	406:430	the most applicable drugs for veterinary and human health purposes	406:471	Diclofenac (DCF) is one of the most applicable drugs for veterinary and human health purposes, polluting surface waters in different ways.					
36179472	2	79	theme	veterinary	436:445	arg1	purposes					464:471	veterinary and human health purposes	436:471	veterinary and human health purposes	436:471	Diclofenac (DCF) is one of the most applicable drugs for veterinary and human health purposes, polluting surface waters in different ways.					
36179472	8	80	theme	excellent	1681:1689	arg1	reusability					1691:1701	excellent reusability	1681:1701	excellent reusability	1681:1701	Finally, the PGMZ photocatalyst was reused six times with a reduction of about 20% in its removal efficiency, indicating excellent reusability and stability.					
36179472	8	81	theme	%	1641:1641	arg1	reduction					1620:1628	a reduction	1618:1628	a reduction of about 20% in its removal efficiency	1618:1667	Finally, the PGMZ photocatalyst was reused six times with a reduction of about 20% in its removal efficiency, indicating excellent reusability and stability.					
36179472	3	82	theme	oxide	571:575	arg1	nanocomposite					627:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite	547:639	a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF	547:684	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	3	82	theme	oxide	571:575	arg1	PGMZ					642:645	PGMZ	642:645	PGMZ	642:645	This work aims to synthesize a novel pectin-graphene oxide (GO)-magnesium ferrite (MgFe2O4)-zinc oxide (ZnO) nanocomposite (PGMZ) for photocatalytic degradation of DCF in an aquatic environment under visible light irradiation.					
36179472	1	83	theme	rising	303:308	arg1	population					310:319	rising population	303:319	rising population	303:319	Wastewater containing pharmaceutical contaminants has become a critical environmental concern due to rising population and drug consumption caused by increased life expectancy.					
36604822	3	0	theme	Gram-negative	505:517	arg1	bacterium					451:459	One bacterium	447:459	One bacterium that possesses this suite of enzymes	447:496	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	3	0	theme	Gram-negative	505:517	arg1	japonicus					541:549	the Gram-negative saprophyte Cellvibrio japonicus	501:549	the Gram-negative saprophyte Cellvibrio japonicus	501:549	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	1	1	theme	substitutions	246:258	arg1	terms					209:213	terms	209:213	terms of its backbone and side-chain substitutions	209:258	Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions.					
36604822	3	2	theme	saprophyte	519:528	arg1	bacterium					451:459	One bacterium	447:459	One bacterium that possesses this suite of enzymes	447:496	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	3	2	theme	saprophyte	519:528	arg1	japonicus					541:549	the Gram-negative saprophyte Cellvibrio japonicus	501:549	the Gram-negative saprophyte Cellvibrio japonicus	501:549	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	6	3	theme	mutational	1002:1011	arg1	analysis					1013:1020	A comprehensive mutational analysis	986:1020	A comprehensive mutational analysis	986:1020	A comprehensive mutational analysis characterized 54 CAZyme-encoding genes in the context of mannan utilization.					
36604822	8	4	theme	degradation	1380:1390	arg1	model					1364:1368	Our updated model	1352:1368	Our updated model of mannan degradation in C. japonicus	1352:1406	Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.					
36604822	8	5	theme	complete	1518:1525	arg1	degradation					1527:1537	the complete degradation	1514:1537	the complete degradation of this hemicellulose	1514:1559	Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.					
36604822	7	6	theme	strains	1129:1135	arg1	analysis					1106:1113	Growth analysis	1099:1113	Growth analysis of the mutant strains	1099:1135	Growth analysis of the mutant strains found that the man26C, aga27A, and man5D genes, which encode a mannobiohydrolase, α-galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential.					
36604822	2	7	theme	mannan	294:299	arg1	substrates					301:310	mannan substrates	294:310	mannan substrates	294:310	Consequently, the degradation of mannan substrates requires a cadre of enzymes for complete reduction to substituent monosaccharides that can include mannose, galactose, and/or glucose.					
36604822	2	8	theme	substrates	301:310	arg1	degradation					279:289	the degradation	275:289	the degradation of mannan substrates	275:310	Consequently, the degradation of mannan substrates requires a cadre of enzymes for complete reduction to substituent monosaccharides that can include mannose, galactose, and/or glucose.					
36604822	6	9	theme	comprehensive	988:1000	arg1	analysis					1013:1020	A comprehensive mutational analysis	986:1020	A comprehensive mutational analysis	986:1020	A comprehensive mutational analysis characterized 54 CAZyme-encoding genes in the context of mannan utilization.					
36604822	8	10	theme	mannan	1373:1378	arg1	degradation					1380:1390	mannan degradation	1373:1390	mannan degradation	1373:1390	Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.					
36604822	5	11	from	genes	889:893	arg1	expression					841:850	gene expression	836:850	gene expression for most mannanases	836:870	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	4	12	theme	biology	672:678	arg1	approach					680:687	a systems biology approach	662:687	a systems biology approach to identify and characterize the essential mannan-degrading components in this bacterium	662:776	Here we describe a systems biology approach to identify and characterize the essential mannan-degrading components in this bacterium.					
36604822	5	13	theme	Active	920:925	arg1	Enzymes					927:933	Carbohydrate Active Enzymes	907:933	Carbohydrate Active Enzymes (CAZymes)	907:943	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	5	13	theme	Active	920:925	arg1	genes					889:893	many genes	884:893	many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded	884:983	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	5	13	theme	Active	920:925	arg1	CAZymes					936:942	CAZymes	936:942	CAZymes	936:942	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	7	14	dep	man26C	1152:1157	arg1	the					1148:1150	the	1148:1150	the	1148:1150	Growth analysis of the mutant strains found that the man26C, aga27A, and man5D genes, which encode a mannobiohydrolase, α-galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential.					
36604822	5	15	theme	most	856:859	arg1	mannanases					861:870	most mannanases	856:870	most mannanases	856:870	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	3	16	from	families	621:628	arg1	mannanases					576:585	ten predicted mannanases	562:585	ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27	562:642	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	0	17	theme	aga27A	110:115	arg1	gene					117:120	the aga27A gene	106:120	the aga27A gene	106:120	Galactomannan utilization by Cellvibrio japonicus relies on a single essential α-galactosidase encoded by the aga27A gene.					
36604822	5	18	theme	Carbohydrate	907:918	arg1	Enzymes					927:933	Carbohydrate Active Enzymes	907:933	Carbohydrate Active Enzymes (CAZymes)	907:943	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	5	18	theme	Carbohydrate	907:918	arg1	genes					889:893	many genes	884:893	many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded	884:983	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	5	18	theme	Carbohydrate	907:918	arg1	CAZymes					936:942	CAZymes	936:942	CAZymes	936:942	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	7	19	theme	mutant	1122:1127	arg1	strains					1129:1135	the mutant strains	1118:1135	the mutant strains	1118:1135	Growth analysis of the mutant strains found that the man26C, aga27A, and man5D genes, which encode a mannobiohydrolase, α-galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential.					
36604822	6	20	theme	CAZyme-encoding	1039:1053	arg1	genes					1055:1059	54 CAZyme-encoding genes	1036:1059	54 CAZyme-encoding genes	1036:1059	A comprehensive mutational analysis characterized 54 CAZyme-encoding genes in the context of mannan utilization.					
36604822	8	21	theme	hemicellulose	1547:1559	arg1	degradation					1527:1537	the complete degradation	1514:1537	the complete degradation of this hemicellulose	1514:1559	Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.					
36604822	8	22	from	mannans	1475:1481	arg1	removal					1426:1432	the removal	1422:1432	the removal of galactose sidechains from substituted mannans	1422:1481	Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.					
36604822	4	23	theme	essential	722:730	arg1	components					749:758	the essential mannan-degrading components	718:758	the essential mannan-degrading components	718:758	Here we describe a systems biology approach to identify and characterize the essential mannan-degrading components in this bacterium.					
36604822	6	24	theme	utilization	1086:1096	arg1	context					1068:1074	the context	1064:1074	the context of mannan utilization	1064:1096	A comprehensive mutational analysis characterized 54 CAZyme-encoding genes in the context of mannan utilization.					
36604822	0	25	theme	Galactomannan	0:12	arg1	utilization					14:24	Galactomannan utilization	0:24	Galactomannan utilization by Cellvibrio japonicus	0:48	Galactomannan utilization by Cellvibrio japonicus relies on a single essential α-galactosidase encoded by the aga27A gene.					
36604822	1	26	theme	diverse	185:191	arg1	compositions					193:204	diverse compositions	185:204	diverse compositions	185:204	Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions.					
36604822	0	27	theme	Cellvibrio	29:38	arg1	japonicus					40:48	Cellvibrio japonicus	29:48	Cellvibrio japonicus	29:48	Galactomannan utilization by Cellvibrio japonicus relies on a single essential α-galactosidase encoded by the aga27A gene.					
36604822	3	28	theme	predicted	566:574	arg1	mannanases					576:585	ten predicted mannanases	562:585	ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27	562:642	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	7	29	theme	galactomannan	1308:1320	arg1	deconstruction					1290:1303	the deconstruction	1286:1303	the deconstruction of galactomannan	1286:1320	Growth analysis of the mutant strains found that the man26C, aga27A, and man5D genes, which encode a mannobiohydrolase, α-galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential.					
36604822	7	30	theme	man5D	1172:1176	arg1	mannosidase					1240:1250	mannosidase	1240:1250	mannosidase	1240:1250	Growth analysis of the mutant strains found that the man26C, aga27A, and man5D genes, which encode a mannobiohydrolase, α-galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential.					
36604822	7	30	theme	man5D	1172:1176	arg1	mannobiohydrolase					1200:1216	a mannobiohydrolase	1198:1216	a mannobiohydrolase	1198:1216	Growth analysis of the mutant strains found that the man26C, aga27A, and man5D genes, which encode a mannobiohydrolase, α-galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential.					
36604822	7	30	theme	man5D	1172:1176	arg1	genes					1178:1182	man5D genes	1172:1182	man5D genes	1172:1182	Growth analysis of the mutant strains found that the man26C, aga27A, and man5D genes, which encode a mannobiohydrolase, α-galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential.					
36604822	7	30	theme	man5D	1172:1176	arg1	α-galactosidase					1219:1233	α-galactosidase	1219:1233	α-galactosidase	1219:1233	Growth analysis of the mutant strains found that the man26C, aga27A, and man5D genes, which encode a mannobiohydrolase, α-galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential.					
36604822	7	30	theme	man5D	1172:1176	arg1	man26C					1152:1157	man26C	1152:1157	man26C	1152:1157	Growth analysis of the mutant strains found that the man26C, aga27A, and man5D genes, which encode a mannobiohydrolase, α-galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential.					
36604822	7	30	theme	man5D	1172:1176	arg1	aga27A					1160:1165	aga27A	1160:1165	aga27A	1160:1165	Growth analysis of the mutant strains found that the man26C, aga27A, and man5D genes, which encode a mannobiohydrolase, α-galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential.					
36604822	7	31	theme	Growth	1099:1104	arg1	analysis					1106:1113	Growth analysis	1099:1113	Growth analysis of the mutant strains	1099:1135	Growth analysis of the mutant strains found that the man26C, aga27A, and man5D genes, which encode a mannobiohydrolase, α-galactosidase, and mannosidase, respectively, were important for the deconstruction of galactomannan, with Aga27A being essential.					
36604822	3	32	contain	possesses	466:474	arg2	suite					481:485	this suite	476:485	this suite of enzymes	476:496	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	3	32	contain	possesses	466:474	arg1	bacterium					451:459	One bacterium	447:459	One bacterium that possesses this suite of enzymes	447:496	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	3	32	contain	possesses	466:474	arg1	japonicus					541:549	the Gram-negative saprophyte Cellvibrio japonicus	501:549	the Gram-negative saprophyte Cellvibrio japonicus	501:549	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	1	33	theme	lignocellulose	156:169	arg1	mannans					129:135	Plant mannans	123:135	Plant mannans	123:135	Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions.					
36604822	1	33	theme	lignocellulose	156:169	arg1	component					143:151	a component	141:151	a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions	141:258	Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions.					
36604822	4	34	theme	systems	664:670	arg1	approach					680:687	a systems biology approach	662:687	a systems biology approach to identify and characterize the essential mannan-degrading components in this bacterium	662:776	Here we describe a systems biology approach to identify and characterize the essential mannan-degrading components in this bacterium.					
36604822	5	35	theme	Transcriptomic	779:792	arg1	analysis					794:801	Transcriptomic analysis	779:801	Transcriptomic analysis	779:801	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	1	36	contain	have	180:183	arg1	mannans					129:135	Plant mannans	123:135	Plant mannans	123:135	Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions.					
36604822	1	36	contain	have	180:183	arg1	component					143:151	a component	141:151	a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions	141:258	Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions.					
36604822	1	36	contain	have	180:183	arg2	compositions					193:204	diverse compositions	185:204	diverse compositions	185:204	Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions.					
36604822	5	37	theme	many	884:887	arg1	Enzymes					927:933	Carbohydrate Active Enzymes	907:933	Carbohydrate Active Enzymes (CAZymes)	907:943	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	5	37	theme	many	884:887	arg1	genes					889:893	many genes	884:893	many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded	884:983	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	8	38	from	model	1364:1368	arg1	japonicus					1398:1406	C. japonicus	1395:1406	C. japonicus	1395:1406	Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.					
36604822	5	39	theme	gene	836:839	arg1	expression					841:850	gene expression	836:850	gene expression for most mannanases	836:870	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	8	40	theme	substituted	1463:1473	arg1	mannans					1475:1481	substituted mannans	1463:1481	substituted mannans	1463:1481	Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.					
36604822	3	41	theme	Glycoside	596:604	arg1	GH					617:618	GH	617:618	GH	617:618	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	3	41	theme	Glycoside	596:604	arg1	Hydrolase					606:614	Glycoside Hydrolase	596:614	the Glycoside Hydrolase (GH) families 5, 26, and 27	592:642	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	5	42	from	changes	825:831	arg1	expression					841:850	gene expression	836:850	gene expression for most mannanases	836:870	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	2	43	theme	complete	344:351	arg1	reduction					353:361	complete reduction	344:361	complete reduction to substituent monosaccharides that can include mannose, galactose, and/or glucose	344:444	Consequently, the degradation of mannan substrates requires a cadre of enzymes for complete reduction to substituent monosaccharides that can include mannose, galactose, and/or glucose.					
36604822	5	44	theme	significant	813:823	arg1	changes					825:831	significant changes	813:831	significant changes in gene expression for most mannanases	813:870	Transcriptomic analysis uncovered significant changes in gene expression for most mannanases, as well as many genes that encode Carbohydrate Active Enzymes (CAZymes) when mannan was actively being degraded.					
36604822	3	45	theme	Hydrolase	606:614	arg1	families					621:628	the Glycoside Hydrolase (GH) families 5, 26, and 27	592:642	the Glycoside Hydrolase (GH) families 5, 26, and 27	592:642	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	1	46	theme	Plant	123:127	arg1	mannans					129:135	Plant mannans	123:135	Plant mannans	123:135	Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions.					
36604822	1	46	theme	Plant	123:127	arg1	component					143:151	a component	141:151	a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions	141:258	Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions.					
36604822	8	47	theme	sidechains	1447:1456	arg1	removal					1426:1432	the removal	1422:1432	the removal of galactose sidechains from substituted mannans	1422:1481	Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.					
36604822	3	48	contain	has	558:560	arg2	mannanases					576:585	ten predicted mannanases	562:585	ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27	562:642	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	3	48	contain	has	558:560	arg1	bacterium					451:459	One bacterium	447:459	One bacterium that possesses this suite of enzymes	447:496	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	3	48	contain	has	558:560	arg1	japonicus					541:549	the Gram-negative saprophyte Cellvibrio japonicus	501:549	the Gram-negative saprophyte Cellvibrio japonicus	501:549	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	2	49	theme	enzymes	332:338	arg1	cadre					323:327	a cadre	321:327	a cadre of enzymes	321:338	Consequently, the degradation of mannan substrates requires a cadre of enzymes for complete reduction to substituent monosaccharides that can include mannose, galactose, and/or glucose.					
36604822	8	50	theme	updated	1356:1362	arg1	model					1364:1368	Our updated model	1352:1368	Our updated model of mannan degradation in C. japonicus	1352:1406	Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.					
36604822	6	51	theme	mannan	1079:1084	arg1	utilization					1086:1096	mannan utilization	1079:1096	mannan utilization	1079:1096	A comprehensive mutational analysis characterized 54 CAZyme-encoding genes in the context of mannan utilization.					
36604822	4	52	theme	mannan-degrading	732:747	arg1	components					749:758	the essential mannan-degrading components	718:758	the essential mannan-degrading components	718:758	Here we describe a systems biology approach to identify and characterize the essential mannan-degrading components in this bacterium.					
36604822	3	53	theme	enzymes	490:496	arg1	suite					481:485	this suite	476:485	this suite of enzymes	476:496	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	1	54	theme	backbone	222:229	arg1	terms					209:213	terms	209:213	terms of its backbone and side-chain substitutions	209:258	Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions.					
36604822	0	55	theme	essential	69:77	arg1	α-galactosidase					79:93	a single essential α-galactosidase	60:93	a single essential α-galactosidase encoded by the aga27A gene	60:120	Galactomannan utilization by Cellvibrio japonicus relies on a single essential α-galactosidase encoded by the aga27A gene.					
36604822	8	56	theme	crucial	1497:1503	arg1	step					1505:1508	a crucial step	1495:1508	a crucial step for the complete degradation of this hemicellulose	1495:1559	Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.					
36604822	8	57	theme	galactose	1437:1445	arg1	sidechains					1447:1456	galactose sidechains	1437:1456	galactose sidechains	1437:1456	Our updated model of mannan degradation in C. japonicus proposes that the removal of galactose sidechains from substituted mannans constitutes a crucial step for the complete degradation of this hemicellulose.					
36604822	3	58	theme	Cellvibrio	530:539	arg1	bacterium					451:459	One bacterium	447:459	One bacterium that possesses this suite of enzymes	447:496	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	3	58	theme	Cellvibrio	530:539	arg1	japonicus					541:549	the Gram-negative saprophyte Cellvibrio japonicus	501:549	the Gram-negative saprophyte Cellvibrio japonicus	501:549	One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27.					
36604822	0	59	theme	single	62:67	arg1	α-galactosidase					79:93	a single essential α-galactosidase	60:93	a single essential α-galactosidase encoded by the aga27A gene	60:120	Galactomannan utilization by Cellvibrio japonicus relies on a single essential α-galactosidase encoded by the aga27A gene.					
36604822	2	60	theme	substituent	366:376	arg1	monosaccharides					378:392	substituent monosaccharides	366:392	substituent monosaccharides that can include mannose, galactose, and/or glucose	366:444	Consequently, the degradation of mannan substrates requires a cadre of enzymes for complete reduction to substituent monosaccharides that can include mannose, galactose, and/or glucose.					
36604822	1	61	theme	side-chain	235:244	arg1	substitutions					246:258	its backbone and side-chain substitutions	218:258	substitutions	246:258	Plant mannans are a component of lignocellulose that can have diverse compositions in terms of its backbone and side-chain substitutions.					
36731368	5	0	theme	NIR	782:784	arg1	radiation					786:794	NIR radiation	782:794	NIR radiation	782:794	BP converts NIR radiation into heat and stimulates Hb to release oxygen in situ.					
36731368	10	1	theme	biopolymers	1386:1396	arg1	properties					1372:1381	the bioactive properties	1358:1381	the bioactive properties of biopolymers	1358:1396	Notably, this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers is not only applicable for fabricating multifunctional wound dressings but also has a great potential in expanding biomedical engineering fields.					
36731368	4	2	theme	quaternized	722:732	arg1	QCS					744:746	charged quaternized chitosan (QCS)	714:747	charged quaternized chitosan (QCS)	714:747	Black phosphorus (BP) nanosheets and hemoglobin (Hb) were self-assembled layerwise onto electrospun poly-l-lactide (PLLA) nanofibers using charged quaternized chitosan (QCS) and hyaluronic acid.					
36731368	8	3	theme	healing	1141:1147	arg1	potentials					1149:1158	the in vivo diabetic wound healing potentials	1114:1158	the in vivo diabetic wound healing potentials	1114:1158	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers were fabricated, and the in vivo diabetic wound healing potentials were tested.					
36731368	10	4	theme	great	1484:1488	arg1	potential					1490:1498	a great potential	1482:1498	a great potential	1482:1498	Notably, this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers is not only applicable for fabricating multifunctional wound dressings but also has a great potential in expanding biomedical engineering fields.					
36731368	9	5	theme	molecular	1177:1185	arg1	mechanism					1187:1195	The molecular mechanism	1173:1195	The molecular mechanism	1173:1195	The molecular mechanism can be partly attributed to the cytokine-cytokine receptor interaction.					
36731368	4	6	theme	charged	714:720	arg1	QCS					744:746	charged quaternized chitosan (QCS)	714:747	charged quaternized chitosan (QCS)	714:747	Black phosphorus (BP) nanosheets and hemoglobin (Hb) were self-assembled layerwise onto electrospun poly-l-lactide (PLLA) nanofibers using charged quaternized chitosan (QCS) and hyaluronic acid.					
36731368	9	7	theme	cytokine-cytokine	1229:1245	arg1	interaction					1256:1266	the cytokine-cytokine receptor interaction	1225:1266	the cytokine-cytokine receptor interaction	1225:1266	The molecular mechanism can be partly attributed to the cytokine-cytokine receptor interaction.					
36731368	9	8	theme	receptor	1247:1254	arg1	interaction					1256:1266	the cytokine-cytokine receptor interaction	1225:1266	the cytokine-cytokine receptor interaction	1225:1266	The molecular mechanism can be partly attributed to the cytokine-cytokine receptor interaction.					
36731368	8	9	theme	composite	1016:1024	arg1	dressings					1032:1040	composite wound dressings	1016:1040	composite wound dressings (coded as PQBH-n) with different numbers of layers	1016:1091	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers were fabricated, and the in vivo diabetic wound healing potentials were tested.					
36731368	6	10	theme	hemostatic	860:869	arg1	material					904:911	a hemostatic and broad-spectrum antibacterial material	858:911	a hemostatic and broad-spectrum antibacterial material	858:911	QCS is a hemostatic and broad-spectrum antibacterial material.					
36731368	6	10	theme	hemostatic	860:869	arg1	QCS					851:853	QCS	851:853	QCS	851:853	QCS is a hemostatic and broad-spectrum antibacterial material.					
36731368	4	11	theme	hyaluronic	753:762	arg1	acid					764:767	hyaluronic acid	753:767	hyaluronic acid	753:767	Black phosphorus (BP) nanosheets and hemoglobin (Hb) were self-assembled layerwise onto electrospun poly-l-lactide (PLLA) nanofibers using charged quaternized chitosan (QCS) and hyaluronic acid.					
36731368	10	12	theme	NIR-assisted	1315:1326	arg1	delivery					1335:1342	NIR-assisted oxygen delivery	1315:1342	NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers	1315:1396	Notably, this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers is not only applicable for fabricating multifunctional wound dressings but also has a great potential in expanding biomedical engineering fields.					
36731368	4	13	theme	electrospun	663:673	arg1	nanofibers					697:706	electrospun poly-l-lactide (PLLA) nanofibers	663:706	electrospun poly-l-lactide (PLLA) nanofibers	663:706	Black phosphorus (BP) nanosheets and hemoglobin (Hb) were self-assembled layerwise onto electrospun poly-l-lactide (PLLA) nanofibers using charged quaternized chitosan (QCS) and hyaluronic acid.					
36731368	8	14	theme	different	1065:1073	arg1	numbers					1075:1081	different numbers	1065:1081	different numbers of layers	1065:1091	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers were fabricated, and the in vivo diabetic wound healing potentials were tested.					
36731368	7	15	theme	bacteria	987:994	arg1	sensitivity					972:982	the sensitivity	968:982	the sensitivity of bacteria to QCS	968:1001	Moderate BP-derived photothermal therapy can increase the sensitivity of bacteria to QCS.					
36731368	2	16	theme	bacterial	287:295	arg1	infection					297:305	bacterial infection	287:305	bacterial infection	287:305	However, the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection often leads to the formation of hyperplastic scars, increasing the clinical demand for wound dressings.					
36731368	8	17	dep	dressings	1032:1040	arg1	coded					1043:1047	coded	1043:1047	coded as PQBH-n	1043:1057	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers were fabricated, and the in vivo diabetic wound healing potentials were tested.					
36731368	10	18	contain	has	1478:1480	arg1	strategy					1297:1304	this comprehensive strategy	1278:1304	this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers	1278:1396	Notably, this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers is not only applicable for fabricating multifunctional wound dressings but also has a great potential in expanding biomedical engineering fields.					
36731368	10	18	contain	has	1478:1480	arg2	potential					1490:1498	a great potential	1482:1498	a great potential	1482:1498	Notably, this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers is not only applicable for fabricating multifunctional wound dressings but also has a great potential in expanding biomedical engineering fields.					
36731368	6	19	theme	antibacterial	890:902	arg1	material					904:911	a hemostatic and broad-spectrum antibacterial material	858:911	a hemostatic and broad-spectrum antibacterial material	858:911	QCS is a hemostatic and broad-spectrum antibacterial material.					
36731368	6	19	theme	antibacterial	890:902	arg1	QCS					851:853	QCS	851:853	QCS	851:853	QCS is a hemostatic and broad-spectrum antibacterial material.					
36731368	10	20	theme	biomedical	1513:1522	arg1	fields					1536:1541	biomedical engineering fields	1513:1541	biomedical engineering fields	1513:1541	Notably, this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers is not only applicable for fabricating multifunctional wound dressings but also has a great potential in expanding biomedical engineering fields.					
36731368	8	21	theme	wound	1026:1030	arg1	dressings					1032:1040	composite wound dressings	1016:1040	composite wound dressings (coded as PQBH-n) with different numbers of layers	1016:1091	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers were fabricated, and the in vivo diabetic wound healing potentials were tested.					
36731368	8	22	dep	in	1118:1119	arg1	diabetic					1126:1133	diabetic	1126:1133	diabetic	1126:1133	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers were fabricated, and the in vivo diabetic wound healing potentials were tested.					
36731368	8	22	dep	in	1118:1119	arg1	vivo					1121:1124	vivo	1121:1124	vivo	1121:1124	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers were fabricated, and the in vivo diabetic wound healing potentials were tested.					
36731368	4	23	theme	poly-l-lactide	675:688	arg1	nanofibers					697:706	electrospun poly-l-lactide (PLLA) nanofibers	663:706	electrospun poly-l-lactide (PLLA) nanofibers	663:706	Black phosphorus (BP) nanosheets and hemoglobin (Hb) were self-assembled layerwise onto electrospun poly-l-lactide (PLLA) nanofibers using charged quaternized chitosan (QCS) and hyaluronic acid.					
36731368	0	24	theme	bioactive	11:19	arg1	properties					21:30	All-in-one bioactive properties	0:30	All-in-one bioactive properties of photothermal	0:46	All-in-one bioactive properties of photothermal nanofibers for accelerating diabetic wound healing.					
36731368	6	25	theme	broad-spectrum	875:888	arg1	material					904:911	a hemostatic and broad-spectrum antibacterial material	858:911	a hemostatic and broad-spectrum antibacterial material	858:911	QCS is a hemostatic and broad-spectrum antibacterial material.					
36731368	6	25	theme	broad-spectrum	875:888	arg1	QCS					851:853	QCS	851:853	QCS	851:853	QCS is a hemostatic and broad-spectrum antibacterial material.					
36731368	8	26	theme	wound	1135:1139	arg1	healing					1141:1147	the in vivo diabetic wound healing	1114:1147	the in vivo diabetic wound healing potentials	1114:1158	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers were fabricated, and the in vivo diabetic wound healing potentials were tested.					
36731368	1	27	theme	Diabetic	100:107	arg1	healing					115:121	Diabetic wound healing	100:121	Diabetic wound healing	100:121	Diabetic wound healing has attracted widespread attention in biomedical engineering.					
36731368	2	28	theme	wound	394:398	arg1	dressings					400:408	wound dressings	394:408	wound dressings	394:408	However, the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection often leads to the formation of hyperplastic scars, increasing the clinical demand for wound dressings.					
36731368	0	29	theme	All-in-one	0:9	arg1	properties					21:30	All-in-one bioactive properties	0:30	All-in-one bioactive properties of photothermal	0:46	All-in-one bioactive properties of photothermal nanofibers for accelerating diabetic wound healing.					
36731368	4	30	theme	phosphorus	581:590	arg1	nanosheets					597:606	Black phosphorus (BP) nanosheets	575:606	Black phosphorus (BP) nanosheets	575:606	Black phosphorus (BP) nanosheets and hemoglobin (Hb) were self-assembled layerwise onto electrospun poly-l-lactide (PLLA) nanofibers using charged quaternized chitosan (QCS) and hyaluronic acid.					
36731368	1	31	theme	wound	109:113	arg1	healing					115:121	Diabetic wound healing	100:121	Diabetic wound healing	100:121	Diabetic wound healing has attracted widespread attention in biomedical engineering.					
36731368	3	32	theme	comprehensive	429:441	arg1	strategy					443:450	a comprehensive strategy	427:450	a comprehensive strategy using near-infrared NIR-assisted oxygen delivery combined with the bioactive nature of biopolymers for remodeling the HME	427:572	Here, we report a comprehensive strategy using near-infrared NIR-assisted oxygen delivery combined with the bioactive nature of biopolymers for remodeling the HME.					
36731368	4	33	theme	Black	575:579	arg1	nanosheets					597:606	Black phosphorus (BP) nanosheets	575:606	Black phosphorus (BP) nanosheets	575:606	Black phosphorus (BP) nanosheets and hemoglobin (Hb) were self-assembled layerwise onto electrospun poly-l-lactide (PLLA) nanofibers using charged quaternized chitosan (QCS) and hyaluronic acid.					
36731368	8	34	theme	dressings	1032:1040	arg1	series					1006:1011	A series	1004:1011	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers	1004:1091	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers were fabricated, and the in vivo diabetic wound healing potentials were tested.					
36731368	10	35	theme	multifunctional	1437:1451	arg1	dressings					1459:1467	multifunctional wound dressings	1437:1467	multifunctional wound dressings	1437:1467	Notably, this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers is not only applicable for fabricating multifunctional wound dressings but also has a great potential in expanding biomedical engineering fields.					
36731368	3	36	theme	oxygen	485:490	arg1	delivery					492:499	near-infrared NIR-assisted oxygen delivery	458:499	near-infrared NIR-assisted oxygen delivery	458:499	Here, we report a comprehensive strategy using near-infrared NIR-assisted oxygen delivery combined with the bioactive nature of biopolymers for remodeling the HME.					
36731368	4	37	theme	BP	593:594	arg1	nanosheets					597:606	Black phosphorus (BP) nanosheets	575:606	Black phosphorus (BP) nanosheets	575:606	Black phosphorus (BP) nanosheets and hemoglobin (Hb) were self-assembled layerwise onto electrospun poly-l-lactide (PLLA) nanofibers using charged quaternized chitosan (QCS) and hyaluronic acid.					
36731368	8	38	theme	layers	1086:1091	arg1	numbers					1075:1081	different numbers	1065:1081	different numbers of layers	1065:1091	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers were fabricated, and the in vivo diabetic wound healing potentials were tested.					
36731368	2	39	theme	hypoxic	204:210	arg1	HME					230:232	HME	230:232	HME	230:232	However, the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection often leads to the formation of hyperplastic scars, increasing the clinical demand for wound dressings.					
36731368	2	39	theme	hypoxic	204:210	arg1	microenvironment					212:227	the harsh hypoxic microenvironment	194:227	the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection	194:305	However, the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection often leads to the formation of hyperplastic scars, increasing the clinical demand for wound dressings.					
36731368	3	40	theme	bioactive	519:527	arg1	nature					529:534	the bioactive nature	515:534	the bioactive nature of biopolymers for remodeling the HME	515:572	Here, we report a comprehensive strategy using near-infrared NIR-assisted oxygen delivery combined with the bioactive nature of biopolymers for remodeling the HME.					
36731368	1	41	theme	biomedical	161:170	arg1	engineering					172:182	biomedical engineering	161:182	biomedical engineering	161:182	Diabetic wound healing has attracted widespread attention in biomedical engineering.					
36731368	2	42	theme	scars	352:356	arg1	formation					326:334	the formation	322:334	the formation of hyperplastic scars	322:356	However, the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection often leads to the formation of hyperplastic scars, increasing the clinical demand for wound dressings.					
36731368	2	43	theme	harsh	198:202	arg1	HME					230:232	HME	230:232	HME	230:232	However, the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection often leads to the formation of hyperplastic scars, increasing the clinical demand for wound dressings.					
36731368	2	43	theme	harsh	198:202	arg1	microenvironment					212:227	the harsh hypoxic microenvironment	194:227	the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection	194:305	However, the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection often leads to the formation of hyperplastic scars, increasing the clinical demand for wound dressings.					
36731368	4	44	theme	PLLA	691:694	arg1	nanofibers					697:706	electrospun poly-l-lactide (PLLA) nanofibers	663:706	electrospun poly-l-lactide (PLLA) nanofibers	663:706	Black phosphorus (BP) nanosheets and hemoglobin (Hb) were self-assembled layerwise onto electrospun poly-l-lactide (PLLA) nanofibers using charged quaternized chitosan (QCS) and hyaluronic acid.					
36731368	3	45	theme	near-infrared	458:470	arg1	delivery					492:499	near-infrared NIR-assisted oxygen delivery	458:499	near-infrared NIR-assisted oxygen delivery	458:499	Here, we report a comprehensive strategy using near-infrared NIR-assisted oxygen delivery combined with the bioactive nature of biopolymers for remodeling the HME.					
36731368	0	46	theme	photothermal	35:46	arg1	properties					21:30	All-in-one bioactive properties	0:30	All-in-one bioactive properties of photothermal	0:46	All-in-one bioactive properties of photothermal nanofibers for accelerating diabetic wound healing.					
36731368	2	47	theme	glucose	251:257	arg1	levels					259:264	high glucose levels	246:264	high glucose levels	246:264	However, the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection often leads to the formation of hyperplastic scars, increasing the clinical demand for wound dressings.					
36731368	3	48	theme	NIR-assisted	472:483	arg1	delivery					492:499	near-infrared NIR-assisted oxygen delivery	458:499	near-infrared NIR-assisted oxygen delivery	458:499	Here, we report a comprehensive strategy using near-infrared NIR-assisted oxygen delivery combined with the bioactive nature of biopolymers for remodeling the HME.					
36731368	10	49	theme	oxygen	1328:1333	arg1	delivery					1335:1342	NIR-assisted oxygen delivery	1315:1342	NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers	1315:1396	Notably, this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers is not only applicable for fabricating multifunctional wound dressings but also has a great potential in expanding biomedical engineering fields.					
36731368	2	50	theme	high	246:249	arg1	levels					259:264	high glucose levels	246:264	high glucose levels	246:264	However, the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection often leads to the formation of hyperplastic scars, increasing the clinical demand for wound dressings.					
36731368	3	51	dep	remodeling	555:564	arg1	HME					570:572	the HME	566:572	remodeling the HME	555:572	Here, we report a comprehensive strategy using near-infrared NIR-assisted oxygen delivery combined with the bioactive nature of biopolymers for remodeling the HME.					
36731368	10	52	theme	bioactive	1362:1370	arg1	properties					1372:1381	the bioactive properties	1358:1381	the bioactive properties of biopolymers	1358:1396	Notably, this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers is not only applicable for fabricating multifunctional wound dressings but also has a great potential in expanding biomedical engineering fields.					
36731368	1	53	theme	widespread	137:146	arg1	attention					148:156	widespread attention	137:156	widespread attention	137:156	Diabetic wound healing has attracted widespread attention in biomedical engineering.					
36731368	7	54	theme	photothermal	934:945	arg1	therapy					947:953	Moderate BP-derived photothermal therapy	914:953	Moderate BP-derived photothermal therapy	914:953	Moderate BP-derived photothermal therapy can increase the sensitivity of bacteria to QCS.					
36731368	10	55	theme	wound	1453:1457	arg1	dressings					1459:1467	multifunctional wound dressings	1437:1467	multifunctional wound dressings	1437:1467	Notably, this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers is not only applicable for fabricating multifunctional wound dressings but also has a great potential in expanding biomedical engineering fields.					
36731368	7	56	link	BP-derived	923:932	arg1	therapy					947:953	Moderate BP-derived photothermal therapy	914:953	Moderate BP-derived photothermal therapy	914:953	Moderate BP-derived photothermal therapy can increase the sensitivity of bacteria to QCS.					
36731368	2	57	theme	hyperplastic	339:350	arg1	scars					352:356	hyperplastic scars	339:356	hyperplastic scars	339:356	However, the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection often leads to the formation of hyperplastic scars, increasing the clinical demand for wound dressings.					
36731368	8	58	theme	in	1118:1119	arg1	healing					1141:1147	the in vivo diabetic wound healing	1114:1147	the in vivo diabetic wound healing potentials	1114:1158	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers were fabricated, and the in vivo diabetic wound healing potentials were tested.					
36731368	10	59	theme	engineering	1524:1534	arg1	fields					1536:1541	biomedical engineering fields	1513:1541	biomedical engineering fields	1513:1541	Notably, this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers is not only applicable for fabricating multifunctional wound dressings but also has a great potential in expanding biomedical engineering fields.					
36731368	7	60	theme	Moderate	914:921	arg1	therapy					947:953	Moderate BP-derived photothermal therapy	914:953	Moderate BP-derived photothermal therapy	914:953	Moderate BP-derived photothermal therapy can increase the sensitivity of bacteria to QCS.					
36731368	0	61	theme	wound	85:89	arg1	healing					91:97	diabetic wound healing	76:97	diabetic wound healing	76:97	All-in-one bioactive properties of photothermal nanofibers for accelerating diabetic wound healing.					
36731368	8	62	with	dressings	1032:1040	arg1	numbers					1075:1081	different numbers	1065:1081	different numbers of layers	1065:1091	A series of composite wound dressings (coded as PQBH-n) with different numbers of layers were fabricated, and the in vivo diabetic wound healing potentials were tested.					
36731368	3	63	theme	biopolymers	539:549	arg1	nature					529:534	the bioactive nature	515:534	the bioactive nature of biopolymers for remodeling the HME	515:572	Here, we report a comprehensive strategy using near-infrared NIR-assisted oxygen delivery combined with the bioactive nature of biopolymers for remodeling the HME.					
36731368	7	64	theme	BP-derived	923:932	arg1	therapy					947:953	Moderate BP-derived photothermal therapy	914:953	Moderate BP-derived photothermal therapy	914:953	Moderate BP-derived photothermal therapy can increase the sensitivity of bacteria to QCS.					
36731368	0	65	theme	diabetic	76:83	arg1	healing					91:97	diabetic wound healing	76:97	diabetic wound healing	76:97	All-in-one bioactive properties of photothermal nanofibers for accelerating diabetic wound healing.					
36731368	2	66	theme	clinical	374:381	arg1	demand					383:388	the clinical demand	370:388	the clinical demand for wound dressings	370:408	However, the harsh hypoxic microenvironment (HME) comprising high glucose levels, local bleeding, and bacterial infection often leads to the formation of hyperplastic scars, increasing the clinical demand for wound dressings.					
36731368	10	67	theme	comprehensive	1283:1295	arg1	strategy					1297:1304	this comprehensive strategy	1278:1304	this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers	1278:1396	Notably, this comprehensive strategy based on NIR-assisted oxygen delivery combined with the bioactive properties of biopolymers is not only applicable for fabricating multifunctional wound dressings but also has a great potential in expanding biomedical engineering fields.					
36731368	4	68	theme	chitosan	734:741	arg1	QCS					744:746	charged quaternized chitosan (QCS)	714:747	charged quaternized chitosan (QCS)	714:747	Black phosphorus (BP) nanosheets and hemoglobin (Hb) were self-assembled layerwise onto electrospun poly-l-lactide (PLLA) nanofibers using charged quaternized chitosan (QCS) and hyaluronic acid.					
36054511	2	0	theme	gel	600:602	arg1	properties					604:613	gel properties	600:613	gel properties	600:613	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	7	1	theme	kinds	1451:1455	arg1	application					1426:1436	the application	1422:1436	the application of different kinds of carrageenan	1422:1470	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	1	2	theme	shrimp	272:277	arg1	MP					301:302	MP	301:302	MP	301:302	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	1	2	theme	shrimp	272:277	arg1	protein					292:298	shrimp myofibrillar protein	272:298	shrimp myofibrillar protein (MP) gelation	272:312	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	0	3	theme	protein	82:88	arg1	properties					48:57	the gelling properties	36:57	the gelling properties of shrimp myofibrillar protein	36:88	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein and their interaction mechanism exploration.					
36054511	1	4	theme	κ-/ι-carrageenan	180:195	arg1	mechanism					167:175	mechanism	167:175	mechanism	167:175	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	1	4	theme	κ-/ι-carrageenan	180:195	arg1	contribution					150:161	contribution	150:161	contribution	150:161	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	6	5	dep	CONCLUSION	1219:1228	arg1	study					1238:1242	this study	1233:1242	this study	1233:1242	CONCLUSION In this study, a composite gel system was constructed based on the interaction of MP and CG.					
36054511	1	6	theme	myofibrillar	279:290	arg1	MP					301:302	MP	301:302	MP	301:302	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	1	6	theme	myofibrillar	279:290	arg1	protein					292:298	shrimp myofibrillar protein	272:298	shrimp myofibrillar protein (MP) gelation	272:312	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	0	7	theme	interaction	100:110	arg1	mechanism					112:120	their interaction mechanism	94:120	their interaction mechanism	94:120	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein and their interaction mechanism exploration.					
36054511	0	8	from	Contribution	0:11	arg1	properties					48:57	the gelling properties	36:57	the gelling properties of shrimp myofibrillar protein	36:88	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein and their interaction mechanism exploration.					
36054511	4	9	theme	more	880:883	arg1	moisture					885:892	more moisture	880:892	more moisture	880:892	However, MP-ιCG stabilized more moisture in the gel network, thereby improving the tenderness of the gel, which might be related to the electrostatic repulsion observed between the sulfate groups of ιCG and the myosin observed by molecular docking.					
36054511	0	10	dep	exploration	122:132	arg1	Contribution					0:11	Contribution	0:11	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein	0:88	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein and their interaction mechanism exploration.					
36054511	0	10	dep	exploration	122:132	arg1	mechanism					112:120	their interaction mechanism	94:120	their interaction mechanism	94:120	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein and their interaction mechanism exploration.					
36054511	7	11	theme	CG-MP	1363:1367	arg1	gels					1369:1372	CG-MP gels	1363:1372	CG-MP gels	1363:1372	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	1	12	theme	protein	292:298	arg1	gelation					305:312	shrimp myofibrillar protein (MP) gelation	272:312	shrimp myofibrillar protein (MP) gelation	272:312	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	1	13	with	mechanism	167:175	arg1	characteristics					227:241	different hydration characteristics	207:241	different hydration characteristics	207:241	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	6	14	theme	composite	1247:1255	arg1	system					1261:1266	a composite gel system	1245:1266	a composite gel system	1245:1266	CONCLUSION In this study, a composite gel system was constructed based on the interaction of MP and CG.					
36054511	1	15	dep	contribution	150:161	arg1	The					146:148	The	146:148	The	146:148	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	2	16	from	decrease	588:595	arg1	properties					604:613	gel properties	600:613	gel properties	600:613	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	1	17	from	mechanism	167:175	arg1	properties					258:267	the gelling properties	246:267	the gelling properties of shrimp myofibrillar protein (MP) gelation	246:312	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	5	18	theme	gel	1203:1205	arg1	properties					1207:1216	gel properties	1203:1216	gel properties	1203:1216	In addition, the β-sheet content and intermolecular interactions might be positively correlated with gel properties.					
36054511	1	19	with	contribution	150:161	arg1	characteristics					227:241	different hydration characteristics	207:241	different hydration characteristics	207:241	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	7	20	theme	specific	1526:1533	arg1	quality					1535:1541	specific quality	1526:1541	specific quality	1526:1541	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	1	21	dep	BACKGROUND	135:144	arg1	studied					318:324	studied	318:324	was studied	314:324	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	2	22	theme	gels	410:413	arg1	RESULTS					327:333	RESULTS The gel strength, water-holding capacity and viscoelastic properties	327:402	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels	327:413	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	2	22	theme	gels	410:413	arg1	strength					343:350	The gel strength	335:350	The gel strength	335:350	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	2	22	theme	gels	410:413	arg1	capacity					367:374	water-holding capacity	353:374	water-holding capacity	353:374	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	7	23	theme	products	1512:1519	arg1	guidance					1409:1416	guidance	1409:1416	guidance for the application of different kinds of carrageenan	1409:1470	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	7	23	theme	products	1512:1519	arg1	development					1480:1490	the development	1476:1490	the development of recombinant meat products with specific quality	1476:1541	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	1	24	from	contribution	150:161	arg1	properties					258:267	the gelling properties	246:267	the gelling properties of shrimp myofibrillar protein (MP) gelation	246:312	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	7	25	theme	gels	1369:1372	arg1	kinds					1354:1358	two kinds	1350:1358	two kinds of CG-MP gels	1350:1372	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	2	26	theme	MP	407:408	arg1	gels					410:413	MP gels	407:413	MP gels	407:413	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	4	27	theme	electrostatic	989:1001	arg1	repulsion					1003:1011	the electrostatic repulsion	985:1011	the electrostatic repulsion observed between the sulfate groups of ιCG and the myosin observed by molecular docking	985:1099	However, MP-ιCG stabilized more moisture in the gel network, thereby improving the tenderness of the gel, which might be related to the electrostatic repulsion observed between the sulfate groups of ιCG and the myosin observed by molecular docking.					
36054511	5	28	theme	intermolecular	1139:1152	arg1	interactions					1154:1165	intermolecular interactions	1139:1165	intermolecular interactions	1139:1165	In addition, the β-sheet content and intermolecular interactions might be positively correlated with gel properties.					
36054511	3	29	theme	shorter	676:682	arg1	distance					693:700	shorter breaking distance	676:700	shorter breaking distance	676:700	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
36054511	6	30	theme	MP	1312:1313	arg1	interaction					1297:1307	the interaction	1293:1307	the interaction of MP and CG	1293:1320	CONCLUSION In this study, a composite gel system was constructed based on the interaction of MP and CG.					
36054511	1	31	theme	different	207:215	arg1	characteristics					227:241	different hydration characteristics	207:241	different hydration characteristics	207:241	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	0	32	theme	κ-/ι-carrageenan	16:31	arg1	Contribution					0:11	Contribution	0:11	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein	0:88	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein and their interaction mechanism exploration.					
36054511	0	32	theme	κ-/ι-carrageenan	16:31	arg1	mechanism					112:120	their interaction mechanism	94:120	their interaction mechanism	94:120	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein and their interaction mechanism exploration.					
36054511	1	33	theme	gelation	305:312	arg1	properties					258:267	the gelling properties	246:267	the gelling properties of shrimp myofibrillar protein (MP) gelation	246:312	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	2	34	theme	network	560:566	arg1	decrease					588:595	a corresponding decrease	572:595	a corresponding decrease in gel properties	572:613	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	2	34	theme	network	560:566	arg1	fragmentation					535:547	fragmentation	535:547	fragmentation of the gel network	535:566	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	3	35	theme	breaking	657:664	arg1	force					666:670	larger breaking force	650:670	larger breaking force	650:670	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
36054511	2	36	dep	RESULTS	327:333	arg1	RESULTS					327:333	RESULTS The gel strength, water-holding capacity and viscoelastic properties	327:402	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels	327:413	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	2	36	dep	RESULTS	327:333	arg1	strength					343:350	The gel strength	335:350	The gel strength	335:350	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	2	36	dep	RESULTS	327:333	arg1	capacity					367:374	water-holding capacity	353:374	water-holding capacity	353:374	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	2	36	dep	RESULTS	327:333	arg1	properties					393:402	viscoelastic properties	380:402	viscoelastic properties	380:402	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	1	37	theme	hydration	217:225	arg1	characteristics					227:241	different hydration characteristics	207:241	different hydration characteristics	207:241	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	0	38	theme	gelling	40:46	arg1	properties					48:57	the gelling properties	36:57	the gelling properties of shrimp myofibrillar protein	36:88	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein and their interaction mechanism exploration.					
36054511	3	39	theme	gel	754:756	arg1	brittleness					735:745	brittleness	735:745	brittleness	735:745	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
36054511	3	39	theme	gel	754:756	arg1	hardness					722:729	hardness	722:729	hardness	722:729	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
36054511	3	39	theme	gel	754:756	arg1	binding					830:836	a tighter binding	820:836	a tighter binding of κCG-myosin	820:850	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
36054511	2	40	theme	gel	556:558	arg1	network					560:566	the gel network	552:566	the gel network	552:566	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	7	41	theme	meat	1507:1510	arg1	products					1512:1519	recombinant meat products	1495:1519	recombinant meat products with specific quality	1495:1541	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	4	42	theme	molecular	1083:1091	arg1	docking					1093:1099	molecular docking	1083:1099	molecular docking	1083:1099	However, MP-ιCG stabilized more moisture in the gel network, thereby improving the tenderness of the gel, which might be related to the electrostatic repulsion observed between the sulfate groups of ιCG and the myosin observed by molecular docking.					
36054511	7	43	theme	kinds	1354:1358	arg1	differences					1335:1345	The quality differences	1323:1345	The quality differences of two kinds of CG-MP gels	1323:1372	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	7	44	with	products	1512:1519	arg1	quality					1535:1541	specific quality	1526:1541	specific quality	1526:1541	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	6	45	theme	CG	1319:1320	arg1	interaction					1297:1307	the interaction	1293:1307	the interaction of MP and CG	1293:1320	CONCLUSION In this study, a composite gel system was constructed based on the interaction of MP and CG.					
36054511	7	46	theme	carrageenan	1460:1470	arg1	kinds					1451:1455	different kinds	1441:1455	different kinds of carrageenan	1441:1470	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	2	47	theme	excessive	506:514	arg1	carrageenan					516:526	excessive carrageenan	506:526	excessive carrageenan	506:526	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	3	48	theme	larger	650:655	arg1	force					666:670	larger breaking force	650:670	larger breaking force	650:670	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
36054511	4	49	theme	gel	901:903	arg1	network					905:911	the gel network	897:911	the gel network	897:911	However, MP-ιCG stabilized more moisture in the gel network, thereby improving the tenderness of the gel, which might be related to the electrostatic repulsion observed between the sulfate groups of ιCG and the myosin observed by molecular docking.					
36054511	2	50	theme	water-holding	353:365	arg1	RESULTS					327:333	RESULTS The gel strength, water-holding capacity and viscoelastic properties	327:402	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels	327:413	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	2	50	theme	water-holding	353:365	arg1	capacity					367:374	water-holding capacity	353:374	water-holding capacity	353:374	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	7	51	theme	different	1441:1449	arg1	kinds					1451:1455	different kinds	1441:1455	different kinds of carrageenan	1441:1470	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	3	52	theme	breaking	684:691	arg1	distance					693:700	shorter breaking distance	676:700	shorter breaking distance	676:700	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
36054511	4	53	theme	gel	954:956	arg1	tenderness					936:945	the tenderness	932:945	the tenderness	932:945	However, MP-ιCG stabilized more moisture in the gel network, thereby improving the tenderness of the gel, which might be related to the electrostatic repulsion observed between the sulfate groups of ιCG and the myosin observed by molecular docking.					
36054511	4	53	theme	gel	954:956	arg1	related					974:980	related	974:980	related	974:980	However, MP-ιCG stabilized more moisture in the gel network, thereby improving the tenderness of the gel, which might be related to the electrostatic repulsion observed between the sulfate groups of ιCG and the myosin observed by molecular docking.					
36054511	6	54	theme	gel	1257:1259	arg1	system					1261:1266	a composite gel system	1245:1266	a composite gel system	1245:1266	CONCLUSION In this study, a composite gel system was constructed based on the interaction of MP and CG.					
36054511	2	55	theme	corresponding	574:586	arg1	decrease					588:595	a corresponding decrease	572:595	a corresponding decrease in gel properties	572:613	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	0	56	from	mechanism	112:120	arg1	properties					48:57	the gelling properties	36:57	the gelling properties of shrimp myofibrillar protein	36:88	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein and their interaction mechanism exploration.					
36054511	8	57	theme	Chemical	1562:1569	arg1	Industry					1571:1578	Chemical Industry	1562:1578	Chemical Industry	1562:1578	© 2022 Society of Chemical Industry.					
36054511	7	58	theme	recombinant	1495:1505	arg1	products					1512:1519	recombinant meat products	1495:1519	recombinant meat products with specific quality	1495:1541	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	4	59	theme	ιCG	1052:1054	arg1	groups					1042:1047	the sulfate groups	1030:1047	the sulfate groups of ιCG	1030:1054	However, MP-ιCG stabilized more moisture in the gel network, thereby improving the tenderness of the gel, which might be related to the electrostatic repulsion observed between the sulfate groups of ιCG and the myosin observed by molecular docking.					
36054511	4	59	theme	ιCG	1052:1054	arg1	ιCG					1052:1054	ιCG	1052:1054	ιCG	1052:1054	However, MP-ιCG stabilized more moisture in the gel network, thereby improving the tenderness of the gel, which might be related to the electrostatic repulsion observed between the sulfate groups of ιCG and the myosin observed by molecular docking.					
36054511	4	59	theme	ιCG	1052:1054	arg1	myosin					1064:1069	the myosin	1060:1069	the myosin observed by molecular docking	1060:1099	However, MP-ιCG stabilized more moisture in the gel network, thereby improving the tenderness of the gel, which might be related to the electrostatic repulsion observed between the sulfate groups of ιCG and the myosin observed by molecular docking.					
36054511	3	60	theme	network	799:805	arg1	skeleton					807:814	a reinforced network skeleton	786:814	a reinforced network skeleton	786:814	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
36054511	7	61	theme	quality	1327:1333	arg1	differences					1335:1345	The quality differences	1323:1345	The quality differences of two kinds of CG-MP gels	1323:1372	The quality differences of two kinds of CG-MP gels were clarified, which will provide guidance for the application of different kinds of carrageenan and the development of recombinant meat products with specific quality.					
36054511	3	62	theme	tighter	822:828	arg1	binding					830:836	a tighter binding	820:836	a tighter binding of κCG-myosin	820:850	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
36054511	2	63	theme	gel	339:341	arg1	RESULTS					327:333	RESULTS The gel strength, water-holding capacity and viscoelastic properties	327:402	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels	327:413	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	2	63	theme	gel	339:341	arg1	strength					343:350	The gel strength	335:350	The gel strength	335:350	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	1	64	theme	gelling	250:256	arg1	properties					258:267	the gelling properties	246:267	the gelling properties of shrimp myofibrillar protein (MP) gelation	246:312	BACKGROUND The contribution and mechanism of κ-/ι-carrageenan (CG) with different hydration characteristics on the gelling properties of shrimp myofibrillar protein (MP) gelation was studied.					
36054511	0	65	theme	myofibrillar	69:80	arg1	protein					82:88	shrimp myofibrillar protein	62:88	shrimp myofibrillar protein	62:88	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein and their interaction mechanism exploration.					
36054511	2	66	from	fragmentation	535:547	arg1	properties					604:613	gel properties	600:613	gel properties	600:613	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	2	67	theme	1.0	446:448	arg1	%					449:449	%	449:449	%	449:449	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	3	68	theme	κCG-myosin	841:850	arg1	brittleness					735:745	brittleness	735:745	brittleness	735:745	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
36054511	3	68	theme	κCG-myosin	841:850	arg1	hardness					722:729	hardness	722:729	hardness	722:729	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
36054511	3	68	theme	κCG-myosin	841:850	arg1	binding					830:836	a tighter binding	820:836	a tighter binding of κCG-myosin	820:850	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
36054511	0	69	theme	shrimp	62:67	arg1	protein					82:88	shrimp myofibrillar protein	62:88	shrimp myofibrillar protein	62:88	Contribution of κ-/ι-carrageenan on the gelling properties of shrimp myofibrillar protein and their interaction mechanism exploration.					
36054511	5	70	theme	β-sheet	1119:1125	arg1	content					1127:1133	the β-sheet content	1115:1133	the β-sheet content	1115:1133	In addition, the β-sheet content and intermolecular interactions might be positively correlated with gel properties.					
36054511	2	71	theme	%	449:449	arg1	P < 0.05					460:467	P < 0.05	460:467	P < 0.05	460:467	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	2	71	theme	%	449:449	arg1	κ-/ι-CG					451:457	1.0% κ-/ι-CG	446:457	1.0% κ-/ι-CG (P < 0.05)	446:468	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	2	72	theme	viscoelastic	380:391	arg1	properties					393:402	viscoelastic properties	380:402	viscoelastic properties	380:402	RESULTS The gel strength, water-holding capacity and viscoelastic properties of MP gels were significantly enhanced by 1.0% κ-/ι-CG (P < 0.05), but the microstructure showed that excessive carrageenan caused fragmentation of the gel network and a corresponding decrease in gel properties.					
36054511	4	73	theme	sulfate	1034:1040	arg1	groups					1042:1047	the sulfate groups	1030:1047	the sulfate groups of ιCG	1030:1054	However, MP-ιCG stabilized more moisture in the gel network, thereby improving the tenderness of the gel, which might be related to the electrostatic repulsion observed between the sulfate groups of ιCG and the myosin observed by molecular docking.					
36054511	4	73	theme	sulfate	1034:1040	arg1	ιCG					1052:1054	ιCG	1052:1054	ιCG	1052:1054	However, MP-ιCG stabilized more moisture in the gel network, thereby improving the tenderness of the gel, which might be related to the electrostatic repulsion observed between the sulfate groups of ιCG and the myosin observed by molecular docking.					
36054511	3	74	theme	reinforced	788:797	arg1	skeleton					807:814	a reinforced network skeleton	786:814	a reinforced network skeleton	786:814	Compared to MP-ιCG, MP-κCG showed larger breaking force and shorter breaking distance, thus enhancing the hardness and brittleness of the gel, which might be ascribed to a reinforced network skeleton and a tighter binding of κCG-myosin.					
35403800	4	0	theme	dynamic	725:731	arg1	PDES					709:712	the elastic ionic conductive PDES	680:712	the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding	680:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	0	theme	dynamic	725:731	arg1	bonding					754:760	dynamic interfacial hydrogen bonding	725:760	dynamic interfacial hydrogen bonding	725:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	1	theme	wide-range	955:964	arg1	%					1046:1046	78.9-90.7%	1037:1046	78.9-90.7%	1037:1046	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	1	theme	wide-range	955:964	arg1	colors					994:999	strain-induced wide-range (0-500%) dynamic structural colors	940:999	strain-induced wide-range (0-500%) dynamic structural colors	940:999	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	3	2	theme	crystal	526:532	arg1	skeleton					534:541	cellulose nanocrystals (CNCs) liquid crystal skeleton	489:541	cellulose nanocrystals (CNCs) liquid crystal skeleton	489:541	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	5	3	theme	flexible	1254:1261	arg1	sensors					1263:1269	widely applicable and highly reliable flexible sensors	1216:1269	widely applicable and highly reliable flexible sensors	1216:1269	Furthermore, the resulting materials exhibit high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities, which make them well-suitable for developing widely applicable and highly reliable flexible sensors.					
35403800	0	4	theme	Self-Healing	93:104	arg1	Ability					106:112	Self-Healing Ability	93:112	Self-Healing Ability	93:112	Polymerizable Deep Eutectic Solvent-Based Skin-Like Elastomers with Dynamic Schemochrome and Self-Healing Ability.					
35403800	5	5	theme	stretch-ability	1100:1114	arg1	abilities					1160:1168	high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities	1095:1168	high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities	1095:1168	Furthermore, the resulting materials exhibit high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities, which make them well-suitable for developing widely applicable and highly reliable flexible sensors.					
35403800	6	6	theme	anti-counterfeit	1452:1467	arg1	labels					1469:1474	anti-counterfeit labels	1452:1474	anti-counterfeit labels	1452:1474	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	2	7	theme	great	384:388	arg1	promise					390:396	great promise	384:396	great promise for next-generation electronic skin materials	384:442	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	3	8	theme	skin-like	643:651	arg1	elastomer					653:661	a skin-like elastomer	641:661	a skin-like elastomer	641:661	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	6	9	theme	dynamic	1357:1363	arg1	schemochrome					1365:1376	wide-range dynamic schemochrome	1346:1376	wide-range dynamic schemochrome	1346:1376	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	5	10	theme	resulting	1067:1075	arg1	materials					1077:1085	the resulting materials	1063:1085	the resulting materials	1063:1085	Furthermore, the resulting materials exhibit high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities, which make them well-suitable for developing widely applicable and highly reliable flexible sensors.					
35403800	2	11	theme	real-time	323:331	arg1	cues					348:351	real-time dynamic visual cues	323:351	real-time dynamic visual cues	323:351	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	5	12	dep	exhibit	1087:1093	arg1	make					1177:1180	make	1177:1180	make them well-suitable for developing widely applicable and highly reliable flexible sensors	1177:1269	Furthermore, the resulting materials exhibit high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities, which make them well-suitable for developing widely applicable and highly reliable flexible sensors.					
35403800	4	13	theme	structural	983:992	arg1	%					1046:1046	78.9-90.7%	1037:1046	78.9-90.7%	1037:1046	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	13	theme	structural	983:992	arg1	colors					994:999	strain-induced wide-range (0-500%) dynamic structural colors	940:999	strain-induced wide-range (0-500%) dynamic structural colors	940:999	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	5	14	theme	strain-sensing	1127:1140	arg1	abilities					1160:1168	high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities	1095:1168	high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities	1095:1168	Furthermore, the resulting materials exhibit high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities, which make them well-suitable for developing widely applicable and highly reliable flexible sensors.					
35403800	4	15	theme	resulting	915:923	arg1	elastomer					925:933	the resulting elastomer	911:933	the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%)	911:1047	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	6	16	theme	wide-range	1346:1355	arg1	schemochrome					1365:1376	wide-range dynamic schemochrome	1346:1376	wide-range dynamic schemochrome	1346:1376	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	3	17	theme	eutectic	579:586	arg1	PDES					597:600	PDES	597:600	PDES	597:600	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	3	17	theme	eutectic	579:586	arg1	solvent					588:594	polymerizable deep eutectic solvent	560:594	polymerizable deep eutectic solvent (PDES)	560:601	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	4	18	theme	excellent	1005:1013	arg1	ability					1028:1034	excellent self-healing ability	1005:1034	excellent self-healing ability	1005:1034	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	6	19	dep	foldable	1482:1489	arg1	displays					1491:1498	displays	1491:1498	displays	1491:1498	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	3	20	theme	nanocrystals	499:510	arg1	skeleton					534:541	cellulose nanocrystals (CNCs) liquid crystal skeleton	489:541	cellulose nanocrystals (CNCs) liquid crystal skeleton	489:541	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	2	21	theme	detection	312:320	arg1	performance					262:272	the intriguing performance	247:272	the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials	247:442	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	2	22	theme	skin	429:432	arg1	materials					434:442	next-generation electronic skin materials	402:442	next-generation electronic skin materials	402:442	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	4	23	theme	elastic	684:690	arg1	PDES					709:712	the elastic ionic conductive PDES	680:712	the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding	680:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	23	theme	elastic	684:690	arg1	bonding					754:760	dynamic interfacial hydrogen bonding	725:760	dynamic interfacial hydrogen bonding	725:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	23	theme	elastic	684:690	arg1	matrix					714:719	matrix	714:719	matrix	714:719	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	5	24	theme	self-adhesive	1146:1158	arg1	abilities					1160:1168	high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities	1095:1168	high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities	1095:1168	Furthermore, the resulting materials exhibit high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities, which make them well-suitable for developing widely applicable and highly reliable flexible sensors.					
35403800	5	25	dep	stretch-ability	1100:1114	arg1	%					1123:1123	1163.7%	1117:1123	1163.7%	1117:1123	Furthermore, the resulting materials exhibit high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities, which make them well-suitable for developing widely applicable and highly reliable flexible sensors.					
35403800	2	26	theme	strain	305:310	arg1	detection					312:320	skin-like steady wide-range strain detection	277:320	skin-like steady wide-range strain detection	277:320	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	1	27	theme	multifunctional	176:190	arg1	materials					200:208	multifunctional sensing materials	176:208	multifunctional sensing materials	176:208	Animal skin is a huge source of inspiration when it comes to multifunctional sensing materials.					
35403800	5	28	theme	reliable	1245:1252	arg1	sensors					1263:1269	widely applicable and highly reliable flexible sensors	1216:1269	widely applicable and highly reliable flexible sensors	1216:1269	Furthermore, the resulting materials exhibit high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities, which make them well-suitable for developing widely applicable and highly reliable flexible sensors.					
35403800	6	29	theme	skin-like	1321:1329	arg1	materials					1331:1339	biomimetic skin-like materials	1310:1339	biomimetic skin-like materials	1310:1339	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	1	30	theme	sensing	192:198	arg1	materials					200:208	multifunctional sensing materials	176:208	multifunctional sensing materials	176:208	Animal skin is a huge source of inspiration when it comes to multifunctional sensing materials.					
35403800	4	31	theme	CNCs-based	817:826	arg1	structure					840:848	CNCs-based cholesteric structure	817:848	CNCs-based cholesteric structure	817:848	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	5	32	theme	applicable	1223:1232	arg1	sensors					1263:1269	widely applicable and highly reliable flexible sensors	1216:1269	widely applicable and highly reliable flexible sensors	1216:1269	Furthermore, the resulting materials exhibit high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities, which make them well-suitable for developing widely applicable and highly reliable flexible sensors.					
35403800	6	33	theme	biomimetic	1310:1319	arg1	materials					1331:1339	biomimetic skin-like materials	1310:1339	biomimetic skin-like materials	1310:1339	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	1	34	theme	Animal	115:120	arg1	source					137:142	a huge source	130:142	a huge source of inspiration	130:157	Animal skin is a huge source of inspiration when it comes to multifunctional sensing materials.					
35403800	1	34	theme	Animal	115:120	arg1	skin					122:125	Animal skin	115:125	Animal skin	115:125	Animal skin is a huge source of inspiration when it comes to multifunctional sensing materials.					
35403800	2	35	theme	electronic	418:427	arg1	materials					434:442	next-generation electronic skin materials	402:442	next-generation electronic skin materials	402:442	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	6	36	theme	proposed	1276:1283	arg1	approach					1285:1292	The proposed approach	1272:1292	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome	1272:1376	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	2	37	theme	visual	341:346	arg1	cues					348:351	real-time dynamic visual cues	323:351	real-time dynamic visual cues	323:351	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	3	38	theme	cellulose	489:497	arg1	CNCs					513:516	CNCs	513:516	CNCs	513:516	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	3	38	theme	cellulose	489:497	arg1	nanocrystals					499:510	cellulose nanocrystals	489:510	cellulose nanocrystals (CNCs) liquid crystal skeleton	489:541	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	2	39	theme	cues	348:351	arg1	performance					262:272	the intriguing performance	247:272	the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials	247:442	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	6	40	theme	diverse	1421:1427	arg1	labels					1469:1474	anti-counterfeit labels	1452:1474	anti-counterfeit labels	1452:1474	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	6	40	theme	diverse	1421:1427	arg1	foldable					1482:1489	soft foldable displays	1477:1498	soft foldable displays	1477:1498	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	6	40	theme	diverse	1421:1427	arg1	devices					1522:1528	wearable optical devices	1505:1528	wearable optical devices	1505:1528	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	6	40	theme	diverse	1421:1427	arg1	applications					1429:1440	diverse applications	1421:1440	diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices	1421:1528	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	2	41	theme	dynamic	333:339	arg1	cues					348:351	real-time dynamic visual cues	323:351	real-time dynamic visual cues	323:351	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	1	42	theme	inspiration	147:157	arg1	source					137:142	a huge source	130:142	a huge source of inspiration	130:157	Animal skin is a huge source of inspiration when it comes to multifunctional sensing materials.					
35403800	1	42	theme	inspiration	147:157	arg1	skin					122:125	Animal skin	115:125	Animal skin	115:125	Animal skin is a huge source of inspiration when it comes to multifunctional sensing materials.					
35403800	2	43	theme	ability	371:377	arg1	performance					262:272	the intriguing performance	247:272	the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials	247:442	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	4	44	theme	dynamic	975:981	arg1	%					1046:1046	78.9-90.7%	1037:1046	78.9-90.7%	1037:1046	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	44	theme	dynamic	975:981	arg1	colors					994:999	strain-induced wide-range (0-500%) dynamic structural colors	940:999	strain-induced wide-range (0-500%) dynamic structural colors	940:999	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	45	theme	ionic	692:696	arg1	PDES					709:712	the elastic ionic conductive PDES	680:712	the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding	680:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	45	theme	ionic	692:696	arg1	bonding					754:760	dynamic interfacial hydrogen bonding	725:760	dynamic interfacial hydrogen bonding	725:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	45	theme	ionic	692:696	arg1	matrix					714:719	matrix	714:719	matrix	714:719	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	3	46	theme	chameleon	478:486	arg1	skins					467:471	the skins	463:471	the skins of a chameleon	463:486	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	4	47	dep	PDES	709:712	arg1	PDES					709:712	the elastic ionic conductive PDES	680:712	the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding	680:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	47	dep	PDES	709:712	arg1	bonding					754:760	dynamic interfacial hydrogen bonding	725:760	dynamic interfacial hydrogen bonding	725:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	47	dep	PDES	709:712	arg1	matrix					714:719	matrix	714:719	matrix	714:719	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	6	48	theme	optical	1514:1520	arg1	devices					1522:1528	wearable optical devices	1505:1528	wearable optical devices	1505:1528	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	2	49	theme	intriguing	251:260	arg1	performance					262:272	the intriguing performance	247:272	the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials	247:442	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	4	50	dep	fragile	853:859	arg1	limitations					800:810	the limitations	796:810	the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%)	796:1047	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	0	51	theme	Dynamic	68:74	arg1	Schemochrome					76:87	Dynamic Schemochrome	68:87	Dynamic Schemochrome	68:87	Polymerizable Deep Eutectic Solvent-Based Skin-Like Elastomers with Dynamic Schemochrome and Self-Healing Ability.					
35403800	4	52	theme	conductive	698:707	arg1	PDES					709:712	the elastic ionic conductive PDES	680:712	the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding	680:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	52	theme	conductive	698:707	arg1	bonding					754:760	dynamic interfacial hydrogen bonding	725:760	dynamic interfacial hydrogen bonding	725:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	52	theme	conductive	698:707	arg1	matrix					714:719	matrix	714:719	matrix	714:719	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	6	53	theme	new	1400:1402	arg1	possibilities					1404:1416	new possibilities	1400:1416	new possibilities	1400:1416	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	5	54	theme	high	1095:1098	arg1	abilities					1160:1168	high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities	1095:1168	high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities	1095:1168	Furthermore, the resulting materials exhibit high stretch-ability (1163.7%), strain-sensing and self-adhesive abilities, which make them well-suitable for developing widely applicable and highly reliable flexible sensors.					
35403800	4	55	theme	helical	869:875	arg1	pitch					877:881	its helical pitch	865:881	its helical pitch	865:881	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	56	theme	hydrogen	745:752	arg1	PDES					709:712	the elastic ionic conductive PDES	680:712	the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding	680:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	56	theme	hydrogen	745:752	arg1	bonding					754:760	dynamic interfacial hydrogen bonding	725:760	dynamic interfacial hydrogen bonding	725:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	6	57	theme	wearable	1505:1512	arg1	devices					1522:1528	wearable optical devices	1505:1528	wearable optical devices	1505:1528	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	2	58	dep	detection	312:320	arg1	promise					390:396	great promise	384:396	great promise for next-generation electronic skin materials	384:442	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	3	59	theme	in	607:608	arg1	polymerization					615:628	in situ polymerization	607:628	in situ polymerization to develop a skin-like elastomer	607:661	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	0	60	with	Deep	14:17	arg1	Schemochrome					76:87	Dynamic Schemochrome	68:87	Dynamic Schemochrome	68:87	Polymerizable Deep Eutectic Solvent-Based Skin-Like Elastomers with Dynamic Schemochrome and Self-Healing Ability.					
35403800	0	60	with	Deep	14:17	arg1	Ability					106:112	Self-Healing Ability	93:112	Self-Healing Ability	93:112	Polymerizable Deep Eutectic Solvent-Based Skin-Like Elastomers with Dynamic Schemochrome and Self-Healing Ability.					
35403800	1	61	theme	huge	132:135	arg1	source					137:142	a huge source	130:142	a huge source of inspiration	130:157	Animal skin is a huge source of inspiration when it comes to multifunctional sensing materials.					
35403800	1	61	theme	huge	132:135	arg1	skin					122:125	Animal skin	115:125	Animal skin	115:125	Animal skin is a huge source of inspiration when it comes to multifunctional sensing materials.					
35403800	4	62	with	elastomer	925:933	arg1	ability					1028:1034	excellent self-healing ability	1005:1034	excellent self-healing ability	1005:1034	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	62	with	elastomer	925:933	arg1	%					1046:1046	78.9-90.7%	1037:1046	78.9-90.7%	1037:1046	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	62	with	elastomer	925:933	arg1	colors					994:999	strain-induced wide-range (0-500%) dynamic structural colors	940:999	strain-induced wide-range (0-500%) dynamic structural colors	940:999	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	63	theme	cholesteric	828:838	arg1	structure					840:848	CNCs-based cholesteric structure	817:848	CNCs-based cholesteric structure	817:848	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	2	64	theme	wide-range	294:303	arg1	detection					312:320	skin-like steady wide-range strain detection	277:320	skin-like steady wide-range strain detection	277:320	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	2	65	theme	self-healing	358:369	arg1	ability					371:377	self-healing ability	358:377	self-healing ability	358:377	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	3	66	theme	polymerizable	560:572	arg1	PDES					597:600	PDES	597:600	PDES	597:600	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	3	66	theme	polymerizable	560:572	arg1	solvent					588:594	polymerizable deep eutectic solvent	560:594	polymerizable deep eutectic solvent (PDES)	560:601	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	2	67	theme	Bioinspired	211:221	arg1	sensors					223:229	Bioinspired sensors	211:229	Bioinspired sensors	211:229	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	2	68	theme	steady	287:292	arg1	detection					312:320	skin-like steady wide-range strain detection	277:320	skin-like steady wide-range strain detection	277:320	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	2	69	theme	skin-like	277:285	arg1	detection					312:320	skin-like steady wide-range strain detection	277:320	skin-like steady wide-range strain detection	277:320	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	2	70	theme	next-generation	402:416	arg1	materials					434:442	next-generation electronic skin materials	402:442	next-generation electronic skin materials	402:442	Bioinspired sensors integrated with the intriguing performance of skin-like steady wide-range strain detection, real-time dynamic visual cues, and self-healing ability hold great promise for next-generation electronic skin materials.					
35403800	3	71	dep	in	607:608	arg1	situ					610:613	situ	610:613	situ	610:613	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	4	72	theme	self-healing	1015:1026	arg1	ability					1028:1034	excellent self-healing ability	1005:1034	excellent self-healing ability	1005:1034	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	3	73	theme	deep	574:577	arg1	PDES					597:600	PDES	597:600	PDES	597:600	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	3	73	theme	deep	574:577	arg1	solvent					588:594	polymerizable deep eutectic solvent	560:594	polymerizable deep eutectic solvent (PDES)	560:601	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	6	74	theme	soft	1477:1480	arg1	foldable					1482:1489	soft foldable displays	1477:1498	soft foldable displays	1477:1498	The proposed approach of constructing biomimetic skin-like materials with wide-range dynamic schemochrome is expected to extend new possibilities in diverse applications including anti-counterfeit labels, soft foldable displays, and wearable optical devices.					
35403800	3	75	theme	liquid	519:524	arg1	skeleton					534:541	cellulose nanocrystals (CNCs) liquid crystal skeleton	489:541	cellulose nanocrystals (CNCs) liquid crystal skeleton	489:541	Here, inspired by the skins of a chameleon, cellulose nanocrystals (CNCs) liquid crystal skeleton is embedded into polymerizable deep eutectic solvent (PDES) via in situ polymerization to develop a skin-like elastomer.					
35403800	4	76	theme	interfacial	733:743	arg1	PDES					709:712	the elastic ionic conductive PDES	680:712	the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding	680:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35403800	4	76	theme	interfacial	733:743	arg1	bonding					754:760	dynamic interfacial hydrogen bonding	725:760	dynamic interfacial hydrogen bonding	725:760	Benefiting from the elastic ionic conductive PDES matrix and dynamic interfacial hydrogen bonding, this strategy has broken through the limitations that CNCs-based cholesteric structure is fragile and its helical pitch is non-adjustable, endowing the resulting elastomer with strain-induced wide-range (0-500%) dynamic structural colors and excellent self-healing ability (78.9-90.7%).					
35716164	0	0	theme	Candida	75:81	arg1	formation					91:99	Candida biofilm formation	75:99	Candida biofilm formation	75:99	Surface modification of poly(methyl-methacrylate) with farnesol to prevent Candida biofilm formation.					
35716164	4	1	theme	modified	688:695	arg1	PMMA_FAR					708:715	PMMA_FAR	708:715	PMMA_FAR	708:715	PMMA was modified with different FAR concentrations (3-12%), and physicochemical properties, antifungal activity and cytotoxicity of these modified materials (PMMA_FAR) were tested.					
35716164	4	1	theme	modified	688:695	arg1	materials					697:705	these modified materials	682:705	these modified materials (PMMA_FAR)	682:716	PMMA was modified with different FAR concentrations (3-12%), and physicochemical properties, antifungal activity and cytotoxicity of these modified materials (PMMA_FAR) were tested.					
35716164	6	2	theme	tested	932:937	arg1	concentrations					939:952	all tested concentrations	928:952	all tested concentrations	928:952	Incorporation of FAR into the polymeric matrix significantly decreased hydrophilicity at all tested concentrations and significantly reduced biofilm and planktonic cells metabolic activity in the early stage of biofilm formation at ≥6% FAR in PMMA.					
35716164	7	3	with	composites	1097:1106	arg1	%					1115:1115	<9%	1113:1115	<9%	1113:1115	PMMA_FAR composites with <9% FAR were non-toxic.					
35716164	6	4	theme	early	1035:1039	arg1	stage					1041:1045	the early stage	1031:1045	the early stage of biofilm formation	1031:1066	Incorporation of FAR into the polymeric matrix significantly decreased hydrophilicity at all tested concentrations and significantly reduced biofilm and planktonic cells metabolic activity in the early stage of biofilm formation at ≥6% FAR in PMMA.					
35716164	1	5	theme	dental	226:231	arg1	material					233:240	a dental material	224:240	a dental material	224:240	Candida albicans promotes biofilm formation on dentures, which compromises the use of poly(methyl-methacrylate) (PMMA) as a dental material.					
35716164	3	6	dep	concentrations	432:445	arg1	%					454:454	0·0125%	448:454	0·0125%	448:454	The tested concentrations (0·0125% and 0·4%) of FAR, 24 h after incubation on YPD agar, inhibited filamentation of C. albicans.					
35716164	3	6	dep	concentrations	432:445	arg1	%					463:463	0·4%	460:463	0·4%	460:463	The tested concentrations (0·0125% and 0·4%) of FAR, 24 h after incubation on YPD agar, inhibited filamentation of C. albicans.					
35716164	1	7	theme	Candida	102:108	arg1	albicans					110:117	Candida albicans	102:117	Candida albicans	102:117	Candida albicans promotes biofilm formation on dentures, which compromises the use of poly(methyl-methacrylate) (PMMA) as a dental material.					
35716164	0	8	theme	biofilm	83:89	arg1	formation					91:99	Candida biofilm formation	75:99	Candida biofilm formation	75:99	Surface modification of poly(methyl-methacrylate) with farnesol to prevent Candida biofilm formation.					
35716164	5	9	attach	presence	735:742	arg1	composites					763:772	PMMA_FAR composites	754:772	PMMA_FAR composites	754:772	The presence of FAR in PMMA_FAR composites was verified by Fourier-transform infrared spectroscopy (FT-IR).					
35716164	5	9	attach	presence	735:742	arg2	FAR					747:749	FAR	747:749	FAR	747:749	The presence of FAR in PMMA_FAR composites was verified by Fourier-transform infrared spectroscopy (FT-IR).					
35716164	6	10	dep	biofilm	980:986	arg1	activity					1019:1026	metabolic activity	1009:1026	metabolic activity	1009:1026	Incorporation of FAR into the polymeric matrix significantly decreased hydrophilicity at all tested concentrations and significantly reduced biofilm and planktonic cells metabolic activity in the early stage of biofilm formation at ≥6% FAR in PMMA.					
35716164	4	11	mod	modified	558:565	arg1	PMMA					549:552	PMMA	549:552	PMMA	549:552	PMMA was modified with different FAR concentrations (3-12%), and physicochemical properties, antifungal activity and cytotoxicity of these modified materials (PMMA_FAR) were tested.					
35716164	4	11	mod	modified	558:565	arg3	%					606:606	3-12%	602:606	3-12%	602:606	PMMA was modified with different FAR concentrations (3-12%), and physicochemical properties, antifungal activity and cytotoxicity of these modified materials (PMMA_FAR) were tested.					
35716164	4	11	mod	modified	558:565	arg3	concentrations					586:599	different FAR concentrations	572:599	different FAR concentrations (3-12%)	572:607	PMMA was modified with different FAR concentrations (3-12%), and physicochemical properties, antifungal activity and cytotoxicity of these modified materials (PMMA_FAR) were tested.					
35716164	6	12	theme	formation	1058:1066	arg1	stage					1041:1045	the early stage	1031:1045	the early stage of biofilm formation	1031:1066	Incorporation of FAR into the polymeric matrix significantly decreased hydrophilicity at all tested concentrations and significantly reduced biofilm and planktonic cells metabolic activity in the early stage of biofilm formation at ≥6% FAR in PMMA.					
35716164	5	13	from	presence	735:742	arg1	composites					763:772	PMMA_FAR composites	754:772	PMMA_FAR composites	754:772	The presence of FAR in PMMA_FAR composites was verified by Fourier-transform infrared spectroscopy (FT-IR).					
35716164	5	14	theme	FAR	747:749	arg1	presence					735:742	The presence	731:742	The presence of FAR in PMMA_FAR composites	731:772	The presence of FAR in PMMA_FAR composites was verified by Fourier-transform infrared spectroscopy (FT-IR).					
35716164	4	15	theme	materials	697:705	arg1	cytotoxicity					666:677	cytotoxicity	666:677	cytotoxicity	666:677	PMMA was modified with different FAR concentrations (3-12%), and physicochemical properties, antifungal activity and cytotoxicity of these modified materials (PMMA_FAR) were tested.					
35716164	4	15	theme	materials	697:705	arg1	properties					630:639	physicochemical properties	614:639	physicochemical properties	614:639	PMMA was modified with different FAR concentrations (3-12%), and physicochemical properties, antifungal activity and cytotoxicity of these modified materials (PMMA_FAR) were tested.					
35716164	4	15	theme	materials	697:705	arg1	activity					653:660	antifungal activity	642:660	antifungal activity	642:660	PMMA was modified with different FAR concentrations (3-12%), and physicochemical properties, antifungal activity and cytotoxicity of these modified materials (PMMA_FAR) were tested.					
35716164	6	16	theme	planktonic	992:1001	arg1	cells					1003:1007	planktonic cells	992:1007	planktonic cells	992:1007	Incorporation of FAR into the polymeric matrix significantly decreased hydrophilicity at all tested concentrations and significantly reduced biofilm and planktonic cells metabolic activity in the early stage of biofilm formation at ≥6% FAR in PMMA.					
35716164	0	17	theme	Surface	0:6	arg1	modification					8:19	Surface modification	0:19	Surface modification of poly(methyl-methacrylate) with farnesol	0:62	Surface modification of poly(methyl-methacrylate) with farnesol to prevent Candida biofilm formation.					
35716164	4	18	theme	different	572:580	arg1	concentrations					586:599	different FAR concentrations	572:599	different FAR concentrations (3-12%)	572:607	PMMA was modified with different FAR concentrations (3-12%), and physicochemical properties, antifungal activity and cytotoxicity of these modified materials (PMMA_FAR) were tested.					
35716164	4	18	theme	different	572:580	arg1	%					606:606	3-12%	602:606	3-12%	602:606	PMMA was modified with different FAR concentrations (3-12%), and physicochemical properties, antifungal activity and cytotoxicity of these modified materials (PMMA_FAR) were tested.					
35716164	2	19	theme	PMMA_FAR	401:408	arg1	materials					410:418	antifungal PMMA_FAR materials	390:418	antifungal PMMA_FAR materials	390:418	Farnesol (FAR), a natural compound that prevents C. albicans filamentation and biofilm formation, was incorporated into the PMMA matrix, to obtain antifungal PMMA_FAR materials.					
35716164	2	20	theme	antifungal	390:399	arg1	materials					410:418	antifungal PMMA_FAR materials	390:418	antifungal PMMA_FAR materials	390:418	Farnesol (FAR), a natural compound that prevents C. albicans filamentation and biofilm formation, was incorporated into the PMMA matrix, to obtain antifungal PMMA_FAR materials.					
35716164	3	21	theme	YPD	499:501	arg1	agar					503:506	YPD agar	499:506	YPD agar	499:506	The tested concentrations (0·0125% and 0·4%) of FAR, 24 h after incubation on YPD agar, inhibited filamentation of C. albicans.					
35716164	8	22	theme	good	1172:1175	arg1	strategy					1177:1184	a good strategy	1170:1184	a good strategy for reducing C. albicans biofilm formation on dentures	1170:1239	Modification of PMMA with FAR is a good strategy for reducing C. albicans biofilm formation on dentures.					
35716164	8	22	theme	good	1172:1175	arg1	Modification					1137:1148	Modification	1137:1148	Modification of PMMA with FAR	1137:1165	Modification of PMMA with FAR is a good strategy for reducing C. albicans biofilm formation on dentures.					
35716164	6	23	theme	metabolic	1009:1017	arg1	activity					1019:1026	metabolic activity	1009:1026	metabolic activity	1009:1026	Incorporation of FAR into the polymeric matrix significantly decreased hydrophilicity at all tested concentrations and significantly reduced biofilm and planktonic cells metabolic activity in the early stage of biofilm formation at ≥6% FAR in PMMA.					
35716164	0	24	theme	poly	24:27	arg1	modification					8:19	Surface modification	0:19	Surface modification of poly(methyl-methacrylate) with farnesol	0:62	Surface modification of poly(methyl-methacrylate) with farnesol to prevent Candida biofilm formation.					
35716164	4	25	theme	antifungal	642:651	arg1	activity					653:660	antifungal activity	642:660	antifungal activity	642:660	PMMA was modified with different FAR concentrations (3-12%), and physicochemical properties, antifungal activity and cytotoxicity of these modified materials (PMMA_FAR) were tested.					
35716164	8	26	theme	biofilm	1211:1217	arg1	formation					1219:1227	C. albicans biofilm formation	1199:1227	C. albicans biofilm formation	1199:1227	Modification of PMMA with FAR is a good strategy for reducing C. albicans biofilm formation on dentures.					
35716164	1	27	theme	poly	188:191	arg1	use					181:183	the use	177:183	the use of poly(methyl-methacrylate) (PMMA) as a dental material	177:240	Candida albicans promotes biofilm formation on dentures, which compromises the use of poly(methyl-methacrylate) (PMMA) as a dental material.					
35716164	2	28	theme	natural	261:267	arg1	Farnesol					243:250	Farnesol	243:250	Farnesol (FAR)	243:256	Farnesol (FAR), a natural compound that prevents C. albicans filamentation and biofilm formation, was incorporated into the PMMA matrix, to obtain antifungal PMMA_FAR materials.					
35716164	2	28	theme	natural	261:267	arg1	compound					269:276	a natural compound	259:276	a natural compound that prevents C. albicans filamentation and biofilm formation	259:338	Farnesol (FAR), a natural compound that prevents C. albicans filamentation and biofilm formation, was incorporated into the PMMA matrix, to obtain antifungal PMMA_FAR materials.					
35716164	6	29	from	%	1073:1073	arg1	PMMA					1082:1085	PMMA	1082:1085	PMMA	1082:1085	Incorporation of FAR into the polymeric matrix significantly decreased hydrophilicity at all tested concentrations and significantly reduced biofilm and planktonic cells metabolic activity in the early stage of biofilm formation at ≥6% FAR in PMMA.					
35716164	2	30	dep	C.	292:293	arg1	albicans					295:302	albicans	295:302	albicans	295:302	Farnesol (FAR), a natural compound that prevents C. albicans filamentation and biofilm formation, was incorporated into the PMMA matrix, to obtain antifungal PMMA_FAR materials.					
35716164	3	31	theme	tested	425:430	arg1	concentrations					432:445	The tested concentrations	421:445	The tested concentrations (0·0125% and 0·4%)	421:464	The tested concentrations (0·0125% and 0·4%) of FAR, 24 h after incubation on YPD agar, inhibited filamentation of C. albicans.					
35716164	6	32	theme	polymeric	869:877	arg1	matrix					879:884	the polymeric matrix	865:884	the polymeric matrix	865:884	Incorporation of FAR into the polymeric matrix significantly decreased hydrophilicity at all tested concentrations and significantly reduced biofilm and planktonic cells metabolic activity in the early stage of biofilm formation at ≥6% FAR in PMMA.					
35716164	2	33	theme	PMMA	367:370	arg1	matrix					372:377	the PMMA matrix	363:377	the PMMA matrix	363:377	Farnesol (FAR), a natural compound that prevents C. albicans filamentation and biofilm formation, was incorporated into the PMMA matrix, to obtain antifungal PMMA_FAR materials.					
35716164	7	34	theme	PMMA_FAR	1088:1095	arg1	composites					1097:1106	PMMA_FAR composites	1088:1106	PMMA_FAR composites with <9%	1088:1115	PMMA_FAR composites with <9% FAR were non-toxic.					
35716164	5	35	theme	PMMA_FAR	754:761	arg1	composites					763:772	PMMA_FAR composites	754:772	PMMA_FAR composites	754:772	The presence of FAR in PMMA_FAR composites was verified by Fourier-transform infrared spectroscopy (FT-IR).					
35716164	8	36	theme	PMMA	1153:1156	arg1	Modification					1137:1148	Modification	1137:1148	Modification of PMMA with FAR	1137:1165	Modification of PMMA with FAR is a good strategy for reducing C. albicans biofilm formation on dentures.					
35716164	8	36	theme	PMMA	1153:1156	arg1	strategy					1177:1184	a good strategy	1170:1184	a good strategy for reducing C. albicans biofilm formation on dentures	1170:1239	Modification of PMMA with FAR is a good strategy for reducing C. albicans biofilm formation on dentures.					
35716164	6	37	theme	biofilm	1050:1056	arg1	formation					1058:1066	biofilm formation	1050:1066	biofilm formation	1050:1066	Incorporation of FAR into the polymeric matrix significantly decreased hydrophilicity at all tested concentrations and significantly reduced biofilm and planktonic cells metabolic activity in the early stage of biofilm formation at ≥6% FAR in PMMA.					
35716164	4	38	theme	physicochemical	614:628	arg1	properties					630:639	physicochemical properties	614:639	physicochemical properties	614:639	PMMA was modified with different FAR concentrations (3-12%), and physicochemical properties, antifungal activity and cytotoxicity of these modified materials (PMMA_FAR) were tested.					
35716164	6	39	theme	FAR	856:858	arg1	Incorporation					839:851	Incorporation	839:851	Incorporation of FAR into the polymeric matrix	839:884	Incorporation of FAR into the polymeric matrix significantly decreased hydrophilicity at all tested concentrations and significantly reduced biofilm and planktonic cells metabolic activity in the early stage of biofilm formation at ≥6% FAR in PMMA.					
35716164	0	40	with	modification	8:19	arg1	farnesol					55:62	farnesol	55:62	farnesol	55:62	Surface modification of poly(methyl-methacrylate) with farnesol to prevent Candida biofilm formation.					
35716164	2	41	theme	C.	292:293	arg1	filamentation					304:316	C. albicans filamentation	292:316	C. albicans filamentation	292:316	Farnesol (FAR), a natural compound that prevents C. albicans filamentation and biofilm formation, was incorporated into the PMMA matrix, to obtain antifungal PMMA_FAR materials.					
35716164	0	42	mod	modification	8:19	arg3	Surface					0:6	Surface modification	0:19	Surface modification of poly(methyl-methacrylate) with farnesol	0:62	Surface modification of poly(methyl-methacrylate) with farnesol to prevent Candida biofilm formation.					
35716164	0	42	mod	modification	8:19	arg1	methyl-methacrylate					29:47	methyl-methacrylate	29:47	methyl-methacrylate	29:47	Surface modification of poly(methyl-methacrylate) with farnesol to prevent Candida biofilm formation.					
35716164	0	42	mod	modification	8:19	arg1	poly					24:27	poly	24:27	poly(methyl-methacrylate)	24:48	Surface modification of poly(methyl-methacrylate) with farnesol to prevent Candida biofilm formation.					
35716164	3	43	theme	albicans	539:546	arg1	filamentation					519:531	filamentation	519:531	filamentation of C. albicans	519:546	The tested concentrations (0·0125% and 0·4%) of FAR, 24 h after incubation on YPD agar, inhibited filamentation of C. albicans.					
35716164	1	44	theme	biofilm	128:134	arg1	formation					136:144	biofilm formation	128:144	biofilm formation	128:144	Candida albicans promotes biofilm formation on dentures, which compromises the use of poly(methyl-methacrylate) (PMMA) as a dental material.					
35716164	3	45	from	incubation	485:494	arg1	agar					503:506	YPD agar	499:506	YPD agar	499:506	The tested concentrations (0·0125% and 0·4%) of FAR, 24 h after incubation on YPD agar, inhibited filamentation of C. albicans.					
35716164	3	46	theme	FAR	469:471	arg1	concentrations					432:445	The tested concentrations	421:445	The tested concentrations (0·0125% and 0·4%)	421:464	The tested concentrations (0·0125% and 0·4%) of FAR, 24 h after incubation on YPD agar, inhibited filamentation of C. albicans.					
35716164	2	47	theme	biofilm	322:328	arg1	formation					330:338	biofilm formation	322:338	biofilm formation	322:338	Farnesol (FAR), a natural compound that prevents C. albicans filamentation and biofilm formation, was incorporated into the PMMA matrix, to obtain antifungal PMMA_FAR materials.					
35716164	5	48	theme	infrared	808:815	arg1	spectroscopy					817:828	Fourier-transform infrared spectroscopy	790:828	Fourier-transform infrared spectroscopy (FT-IR)	790:836	The presence of FAR in PMMA_FAR composites was verified by Fourier-transform infrared spectroscopy (FT-IR).					
35716164	5	48	theme	infrared	808:815	arg1	FT-IR					831:835	FT-IR	831:835	FT-IR	831:835	The presence of FAR in PMMA_FAR composites was verified by Fourier-transform infrared spectroscopy (FT-IR).					
35716164	5	49	theme	Fourier-transform	790:806	arg1	spectroscopy					817:828	Fourier-transform infrared spectroscopy	790:828	Fourier-transform infrared spectroscopy (FT-IR)	790:836	The presence of FAR in PMMA_FAR composites was verified by Fourier-transform infrared spectroscopy (FT-IR).					
35716164	5	49	theme	Fourier-transform	790:806	arg1	FT-IR					831:835	FT-IR	831:835	FT-IR	831:835	The presence of FAR in PMMA_FAR composites was verified by Fourier-transform infrared spectroscopy (FT-IR).					
35716164	8	50	dep	C.	1199:1200	arg1	albicans					1202:1209	albicans	1202:1209	albicans	1202:1209	Modification of PMMA with FAR is a good strategy for reducing C. albicans biofilm formation on dentures.					
35716164	3	51	theme	C.	536:537	arg1	albicans					539:546	C. albicans	536:546	C. albicans	536:546	The tested concentrations (0·0125% and 0·4%) of FAR, 24 h after incubation on YPD agar, inhibited filamentation of C. albicans.					
35716164	8	52	with	Modification	1137:1148	arg1	FAR					1163:1165	FAR	1163:1165	FAR	1163:1165	Modification of PMMA with FAR is a good strategy for reducing C. albicans biofilm formation on dentures.					
35716164	2	53	dep	Farnesol	243:250	arg1	FAR					253:255	FAR	253:255	FAR	253:255	Farnesol (FAR), a natural compound that prevents C. albicans filamentation and biofilm formation, was incorporated into the PMMA matrix, to obtain antifungal PMMA_FAR materials.					
35716164	8	54	theme	C.	1199:1200	arg1	formation					1219:1227	C. albicans biofilm formation	1199:1227	C. albicans biofilm formation	1199:1227	Modification of PMMA with FAR is a good strategy for reducing C. albicans biofilm formation on dentures.					
37365335	0	0	theme	natural	87:93	arg1	biosynthesis					103:114	natural product biosynthesis	87:114	natural product biosynthesis	87:114	Tandem intermolecular [4 + 2] cycloadditions are catalysed by glycosylated enzymes for natural product biosynthesis.					
37365335	7	1	theme	artificial	1296:1305	arg1	design					1307:1312	the artificial design	1292:1312	the artificial design of biocatalysts	1292:1328	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	7	2	theme	complex	1204:1210	arg1	reactions					1219:1227	complex tandem reactions	1204:1227	complex tandem reactions	1204:1227	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	7	3	theme	synergistic	1069:1079	arg1	effect					1081:1086	The synergistic effect	1065:1086	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions,	1065:1228	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	7	4	theme	tandem	1212:1217	arg1	reactions					1219:1227	complex tandem reactions	1204:1227	complex tandem reactions	1204:1227	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	6	5	theme	calcium	913:919	arg1	ion					921:923	the calcium ion	909:923	the calcium ion	909:923	The N-glycan at N211 in PycR1 significantly increases the affinity to the calcium ion, which in turn regulates the active cavity, making it specifically interact with substrates to accelerate the tandem [4 + 2] cycloaddition.					
37365335	5	6	theme	diverse	820:826	arg1	N-glycans					828:836	diverse N-glycans	820:836	diverse N-glycans	820:836	These enzymes are secreted as glycoproteins with diverse N-glycans.					
37365335	3	7	theme	calcium-ion-dependent	404:424	arg1	enzymes					439:445	two calcium-ion-dependent glycosylated enzymes	400:445	two calcium-ion-dependent glycosylated enzymes	400:445	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	3	7	theme	calcium-ion-dependent	404:424	arg1	PycR1					458:462	PycR1	458:462	PycR1	458:462	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	3	7	theme	calcium-ion-dependent	404:424	arg1	EupfF					448:452	EupfF	448:452	EupfF	448:452	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	1	8	from	construction	173:184	arg1	compounds					232:240	complex organic compounds	216:240	complex organic compounds	216:240	Tandem Diels-Alder reactions are frequently used in the construction of polycyclic ring systems in complex organic compounds.					
37365335	2	9	theme	single	300:305	arg1	cycloaddition					307:319	a single cycloaddition	298:319	a single cycloaddition	298:319	Unlike the many Diels-Alderases (DAases) that catalyse a single cycloaddition, enzymes for multiple Diels-Alder reactions are rare.					
37365335	0	10	theme	product	95:101	arg1	biosynthesis					103:114	natural product biosynthesis	87:114	natural product biosynthesis	87:114	Tandem intermolecular [4 + 2] cycloadditions are catalysed by glycosylated enzymes for natural product biosynthesis.					
37365335	4	11	theme	structures	707:716	arg1	analysis					677:684	analysis	677:684	analysis of enzyme co-crystal structures, together with computational and mutational studies	677:768	We elucidate the origins of catalysis and stereoselectivity within these DAases through analysis of enzyme co-crystal structures, together with computational and mutational studies.					
37365335	3	12	gly	glycosylated	426:437	arg1	enzymes					439:445	two calcium-ion-dependent glycosylated enzymes	400:445	two calcium-ion-dependent glycosylated enzymes	400:445	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	3	12	gly	glycosylated	426:437	arg1	PycR1					458:462	PycR1	458:462	PycR1	458:462	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	3	12	gly	glycosylated	426:437	arg1	EupfF					448:452	EupfF	448:452	EupfF	448:452	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	5	13	gly	glycoproteins	801:813	arg1	enzymes					777:783	These enzymes	771:783	These enzymes	771:783	These enzymes are secreted as glycoproteins with diverse N-glycans.					
37365335	5	13	gly	glycoproteins	801:813	arg1	glycoproteins					801:813	glycoproteins	801:813	glycoproteins with diverse N-glycans	801:836	These enzymes are secreted as glycoproteins with diverse N-glycans.					
37365335	7	14	theme	evolution	1270:1278	arg1	understanding					1245:1257	our understanding	1241:1257	our understanding of protein evolution	1241:1278	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	3	15	theme	intermolecular	500:513	arg1	reactions					527:535	sequential, intermolecular Diels-Alder reactions	488:535	reactions	527:535	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	4	16	theme	studies	762:768	arg1	analysis					677:684	analysis	677:684	analysis of enzyme co-crystal structures, together with computational and mutational studies	677:768	We elucidate the origins of catalysis and stereoselectivity within these DAases through analysis of enzyme co-crystal structures, together with computational and mutational studies.					
37365335	7	17	from	effect	1081:1086	arg1	centre					1137:1142	the catalytic centre	1123:1142	the catalytic centre	1123:1142	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	1	18	used	used	161:164	arg2	reactions					136:144	Tandem Diels-Alder reactions	117:144	Tandem Diels-Alder reactions	117:144	Tandem Diels-Alder reactions are frequently used in the construction of polycyclic ring systems in complex organic compounds.					
37365335	1	19	theme	polycyclic	189:198	arg1	systems					205:211	polycyclic ring systems	189:211	polycyclic ring systems in complex organic compounds	189:240	Tandem Diels-Alder reactions are frequently used in the construction of polycyclic ring systems in complex organic compounds.					
37365335	0	20	theme	intermolecular	7:20	arg1	cycloadditions					30:43	Tandem intermolecular [4 + 2] cycloadditions	0:43	Tandem intermolecular [4 + 2] cycloadditions	0:43	Tandem intermolecular [4 + 2] cycloadditions are catalysed by glycosylated enzymes for natural product biosynthesis.					
37365335	3	21	from	reactions	527:535	arg1	biosynthesis					544:555	the biosynthesis	540:555	the biosynthesis of bistropolone-sesquiterpenes	540:586	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	1	22	theme	ring	200:203	arg1	systems					205:211	polycyclic ring systems	189:211	polycyclic ring systems in complex organic compounds	189:240	Tandem Diels-Alder reactions are frequently used in the construction of polycyclic ring systems in complex organic compounds.					
37365335	0	23	theme	Tandem	0:5	arg1	cycloadditions					30:43	Tandem intermolecular [4 + 2] cycloadditions	0:43	Tandem intermolecular [4 + 2] cycloadditions	0:43	Tandem intermolecular [4 + 2] cycloadditions are catalysed by glycosylated enzymes for natural product biosynthesis.					
37365335	4	24	theme	catalysis	617:625	arg1	origins					606:612	the origins	602:612	the origins of catalysis and stereoselectivity	602:647	We elucidate the origins of catalysis and stereoselectivity within these DAases through analysis of enzyme co-crystal structures, together with computational and mutational studies.					
37365335	7	25	theme	secondary	1167:1175	arg1	metabolism					1177:1186	secondary metabolism	1167:1186	secondary metabolism	1167:1186	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	6	26	from	N211	855:858	arg1	N-glycan					843:850	The N-glycan	839:850	The N-glycan at N211 in PycR1	839:867	The N-glycan at N211 in PycR1 significantly increases the affinity to the calcium ion, which in turn regulates the active cavity, making it specifically interact with substrates to accelerate the tandem [4 + 2] cycloaddition.					
37365335	1	27	theme	systems	205:211	arg1	construction					173:184	the construction	169:184	the construction of polycyclic ring systems in complex organic compounds	169:240	Tandem Diels-Alder reactions are frequently used in the construction of polycyclic ring systems in complex organic compounds.					
37365335	7	28	theme	enzymes	1147:1153	arg1	centre					1137:1142	the catalytic centre	1123:1142	the catalytic centre	1123:1142	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	5	29	with	glycoproteins	801:813	arg1	N-glycans					828:836	diverse N-glycans	820:836	diverse N-glycans	820:836	These enzymes are secreted as glycoproteins with diverse N-glycans.					
37365335	4	30	theme	stereoselectivity	631:647	arg1	origins					606:612	the origins	602:612	the origins of catalysis and stereoselectivity	602:647	We elucidate the origins of catalysis and stereoselectivity within these DAases through analysis of enzyme co-crystal structures, together with computational and mutational studies.					
37365335	6	31	theme	active	954:959	arg1	cavity					961:966	the active cavity	950:966	the active cavity	950:966	The N-glycan at N211 in PycR1 significantly increases the affinity to the calcium ion, which in turn regulates the active cavity, making it specifically interact with substrates to accelerate the tandem [4 + 2] cycloaddition.					
37365335	1	32	theme	Tandem	117:122	arg1	reactions					136:144	Tandem Diels-Alder reactions	117:144	Tandem Diels-Alder reactions	117:144	Tandem Diels-Alder reactions are frequently used in the construction of polycyclic ring systems in complex organic compounds.					
37365335	1	33	theme	complex	216:222	arg1	compounds					232:240	complex organic compounds	216:240	complex organic compounds	216:240	Tandem Diels-Alder reactions are frequently used in the construction of polycyclic ring systems in complex organic compounds.					
37365335	6	34	theme	[4 + 2	1042:1047	arg1	cycloaddition					1050:1062	the tandem [4 + 2] cycloaddition	1031:1062	the tandem [4 + 2] cycloaddition	1031:1062	The N-glycan at N211 in PycR1 significantly increases the affinity to the calcium ion, which in turn regulates the active cavity, making it specifically interact with substrates to accelerate the tandem [4 + 2] cycloaddition.					
37365335	7	35	theme	protein	1262:1268	arg1	evolution					1270:1278	protein evolution	1262:1278	protein evolution	1262:1278	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	2	36	theme	many	254:257	arg1	DAases					276:281	the many Diels-Alderases (DAases)	250:282	the many Diels-Alderases (DAases) that catalyse a single cycloaddition	250:319	Unlike the many Diels-Alderases (DAases) that catalyse a single cycloaddition, enzymes for multiple Diels-Alder reactions are rare.					
37365335	4	37	theme	mutational	751:760	arg1	studies					762:768	computational and mutational studies	733:768	computational and mutational studies	733:768	We elucidate the origins of catalysis and stereoselectivity within these DAases through analysis of enzyme co-crystal structures, together with computational and mutational studies.					
37365335	1	38	theme	organic	224:230	arg1	compounds					232:240	complex organic compounds	216:240	complex organic compounds	216:240	Tandem Diels-Alder reactions are frequently used in the construction of polycyclic ring systems in complex organic compounds.					
37365335	6	39	theme	tandem	1035:1040	arg1	cycloaddition					1050:1062	the tandem [4 + 2] cycloaddition	1031:1062	the tandem [4 + 2] cycloaddition	1031:1062	The N-glycan at N211 in PycR1 significantly increases the affinity to the calcium ion, which in turn regulates the active cavity, making it specifically interact with substrates to accelerate the tandem [4 + 2] cycloaddition.					
37365335	4	40	dep	together	719:726	arg1	with					728:731	with	728:731	with	728:731	We elucidate the origins of catalysis and stereoselectivity within these DAases through analysis of enzyme co-crystal structures, together with computational and mutational studies.					
37365335	7	41	theme	ion	1103:1105	arg1	effect					1081:1086	The synergistic effect	1065:1086	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions,	1065:1228	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	3	42	theme	sequential	488:497	arg1	reactions					527:535	sequential, intermolecular Diels-Alder reactions	488:535	reactions	527:535	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	3	43	theme	glycosylated	426:437	arg1	enzymes					439:445	two calcium-ion-dependent glycosylated enzymes	400:445	two calcium-ion-dependent glycosylated enzymes	400:445	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	3	43	theme	glycosylated	426:437	arg1	PycR1					458:462	PycR1	458:462	PycR1	458:462	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	3	43	theme	glycosylated	426:437	arg1	EupfF					448:452	EupfF	448:452	EupfF	448:452	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	6	44	dep	making	969:974	arg1	interact					992:999	interact	992:999	interact with substrates to accelerate the tandem [4 + 2] cycloaddition	992:1062	The N-glycan at N211 in PycR1 significantly increases the affinity to the calcium ion, which in turn regulates the active cavity, making it specifically interact with substrates to accelerate the tandem [4 + 2] cycloaddition.					
37365335	7	45	theme	biocatalysts	1317:1328	arg1	design					1307:1312	the artificial design	1292:1312	the artificial design of biocatalysts	1292:1328	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	1	46	from	compounds	232:240	arg1	construction					173:184	the construction	169:184	the construction of polycyclic ring systems in complex organic compounds	169:240	Tandem Diels-Alder reactions are frequently used in the construction of polycyclic ring systems in complex organic compounds.					
37365335	1	47	theme	Diels-Alder	124:134	arg1	reactions					136:144	Tandem Diels-Alder reactions	117:144	Tandem Diels-Alder reactions	117:144	Tandem Diels-Alder reactions are frequently used in the construction of polycyclic ring systems in complex organic compounds.					
37365335	7	48	theme	catalytic	1127:1135	arg1	centre					1137:1142	the catalytic centre	1123:1142	the catalytic centre	1123:1142	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	6	49	from	N-glycan	843:850	arg1	PycR1					863:867	PycR1	863:867	PycR1	863:867	The N-glycan at N211 in PycR1 significantly increases the affinity to the calcium ion, which in turn regulates the active cavity, making it specifically interact with substrates to accelerate the tandem [4 + 2] cycloaddition.					
37365335	7	50	theme	N-glycan	1111:1118	arg1	effect					1081:1086	The synergistic effect	1065:1086	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions,	1065:1228	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	7	51	theme	calcium	1095:1101	arg1	ion					1103:1105	the calcium ion	1091:1105	the calcium ion	1091:1105	The synergistic effect of the calcium ion and N-glycan on the catalytic centre of enzymes involved in secondary metabolism, especially for complex tandem reactions, can extend our understanding of protein evolution and improve the artificial design of biocatalysts.					
37365335	2	52	theme	Diels-Alder	343:353	arg1	reactions					355:363	multiple Diels-Alder reactions	334:363	multiple Diels-Alder reactions	334:363	Unlike the many Diels-Alderases (DAases) that catalyse a single cycloaddition, enzymes for multiple Diels-Alder reactions are rare.					
37365335	0	53	gly	glycosylated	62:73	arg1	enzymes					75:81	glycosylated enzymes	62:81	glycosylated enzymes for natural product biosynthesis	62:114	Tandem intermolecular [4 + 2] cycloadditions are catalysed by glycosylated enzymes for natural product biosynthesis.					
37365335	2	54	theme	multiple	334:341	arg1	reactions					355:363	multiple Diels-Alder reactions	334:363	multiple Diels-Alder reactions	334:363	Unlike the many Diels-Alderases (DAases) that catalyse a single cycloaddition, enzymes for multiple Diels-Alder reactions are rare.					
37365335	3	55	theme	Diels-Alder	515:525	arg1	reactions					527:535	sequential, intermolecular Diels-Alder reactions	488:535	reactions	527:535	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	1	56	from	systems	205:211	arg1	compounds					232:240	complex organic compounds	216:240	complex organic compounds	216:240	Tandem Diels-Alder reactions are frequently used in the construction of polycyclic ring systems in complex organic compounds.					
37365335	3	57	theme	bistropolone-sesquiterpenes	560:586	arg1	biosynthesis					544:555	the biosynthesis	540:555	the biosynthesis of bistropolone-sesquiterpenes	540:586	Here we demonstrate that two calcium-ion-dependent glycosylated enzymes, EupfF and PycR1, independently catalyse sequential, intermolecular Diels-Alder reactions in the biosynthesis of bistropolone-sesquiterpenes.					
37365335	4	58	theme	computational	733:745	arg1	studies					762:768	computational and mutational studies	733:768	computational and mutational studies	733:768	We elucidate the origins of catalysis and stereoselectivity within these DAases through analysis of enzyme co-crystal structures, together with computational and mutational studies.					
37365335	4	59	theme	co-crystal	696:705	arg1	structures					707:716	enzyme co-crystal structures	689:716	enzyme co-crystal structures	689:716	We elucidate the origins of catalysis and stereoselectivity within these DAases through analysis of enzyme co-crystal structures, together with computational and mutational studies.					
37365335	0	60	theme	glycosylated	62:73	arg1	enzymes					75:81	glycosylated enzymes	62:81	glycosylated enzymes for natural product biosynthesis	62:114	Tandem intermolecular [4 + 2] cycloadditions are catalysed by glycosylated enzymes for natural product biosynthesis.					
37365335	2	61	theme	Diels-Alderases	259:273	arg1	DAases					276:281	the many Diels-Alderases (DAases)	250:282	the many Diels-Alderases (DAases) that catalyse a single cycloaddition	250:319	Unlike the many Diels-Alderases (DAases) that catalyse a single cycloaddition, enzymes for multiple Diels-Alder reactions are rare.					
37365335	4	62	theme	enzyme	689:694	arg1	structures					707:716	enzyme co-crystal structures	689:716	enzyme co-crystal structures	689:716	We elucidate the origins of catalysis and stereoselectivity within these DAases through analysis of enzyme co-crystal structures, together with computational and mutational studies.					
36827935	6	0	theme	microstructural	1407:1421	arg1	model					1437:1441	a microstructural computational model	1405:1441	a microstructural computational model	1405:1441	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	5	1	theme	bulk	1134:1137	arg1	tests					1139:1143	bulk tests	1134:1143	bulk tests	1134:1143	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	2	2	theme	deformation	370:380	arg1	mechanisms					382:391	the deformation mechanisms	366:391	the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents	366:464	While changes in the structure and composition of tendon have been extensively studied, the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents are not well understood, partly due to the lack of appropriate techniques to probe the behavior.					
36827935	1	3	theme	tissue	270:275	arg1	function					246:253	function	246:253	function	246:253	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	1	3	theme	tissue	270:275	arg1	behavior					233:240	the mechanical behavior	218:240	the mechanical behavior	218:240	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	0	4	theme	fibrils	99:105	arg1	interactions					74:85	the glycosaminoglycan-mediated interactions	43:85	the glycosaminoglycan-mediated interactions of collagen fibrils	43:105	An indentation-based framework for probing the glycosaminoglycan-mediated interactions of collagen fibrils.					
36827935	9	5	from	function	1930:1937	arg1	tissue					1988:1993	collagenous tissue	1976:1993	collagenous tissue	1976:1993	This study reveals the mechanisms that control the orientation-dependent indentation response by affecting the shear deformation and provides new insights into the mechanical function of GAGs and collagen crosslinkers in collagenous tissue.					
36827935	6	6	theme	longitudinal	1304:1315	arg1	orientations					1317:1328	transverse and longitudinal orientations	1289:1328	transverse and longitudinal orientations	1289:1328	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	2	7	dep	structure	299:307	arg1	the					295:297	the	295:297	the	295:297	While changes in the structure and composition of tendon have been extensively studied, the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents are not well understood, partly due to the lack of appropriate techniques to probe the behavior.					
36827935	4	8	contain	have	834:837	arg1	GAGs					829:832	GAGs	829:832	GAGs	829:832	Some studies suggest that GAGs act as crosslinkers between the collagen fibrils, while others have not found such evidence and postulate that GAGs have other functions.					
36827935	4	8	contain	have	834:837	arg2	functions					845:853	other functions	839:853	other functions	839:853	Some studies suggest that GAGs act as crosslinkers between the collagen fibrils, while others have not found such evidence and postulate that GAGs have other functions.					
36827935	5	9	theme	modeling	974:981	arg1	behavior					937:944	orientation-dependent indentation behavior	903:944	orientation-dependent indentation behavior of tissue and computational modeling	903:981	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	6	10	from	GAGs	1215:1218	arg1	tendon					1223:1228	tendon	1223:1228	tendon	1223:1228	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	8	11	theme	microscale	1703:1712	arg1	behavior					1745:1752	their microscale, strain-dependent viscoelastic behavior	1697:1752	their microscale, strain-dependent viscoelastic behavior	1697:1752	We suggest that the molecular reconfiguration of GAGs is a potential mechanism for their microscale, strain-dependent viscoelastic behavior.					
36827935	2	12	theme	techniques	529:538	arg1	lack					509:512	the lack	505:512	the lack of appropriate techniques to probe the behavior	505:560	While changes in the structure and composition of tendon have been extensively studied, the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents are not well understood, partly due to the lack of appropriate techniques to probe the behavior.					
36827935	5	13	theme	relevant	1102:1109	arg1	scale					1091:1095	a length scale	1082:1095	a length scale more relevant to fibrils compared to bulk tests	1082:1143	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	6	14	theme	transverse	1289:1298	arg1	orientations					1317:1328	transverse and longitudinal orientations	1289:1328	transverse and longitudinal orientations	1289:1328	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	9	15	theme	shear	1866:1870	arg1	deformation					1872:1882	the shear deformation	1862:1882	the shear deformation	1862:1882	This study reveals the mechanisms that control the orientation-dependent indentation response by affecting the shear deformation and provides new insights into the mechanical function of GAGs and collagen crosslinkers in collagenous tissue.					
36827935	6	16	from	response	1277:1284	arg1	orientations					1317:1328	transverse and longitudinal orientations	1289:1328	transverse and longitudinal orientations	1289:1328	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	2	17	theme	appropriate	517:527	arg1	techniques					529:538	appropriate techniques	517:538	appropriate techniques	517:538	While changes in the structure and composition of tendon have been extensively studied, the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents are not well understood, partly due to the lack of appropriate techniques to probe the behavior.					
36827935	4	18	theme	collagen	750:757	arg1	fibrils					759:765	the collagen fibrils	746:765	the collagen fibrils	746:765	Some studies suggest that GAGs act as crosslinkers between the collagen fibrils, while others have not found such evidence and postulate that GAGs have other functions.					
36827935	6	19	dep	mechanisms	1368:1377	arg1	the					1341:1343	the	1341:1343	the	1341:1343	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	4	20	theme	other	839:843	arg1	functions					845:853	other functions	839:853	other functions	839:853	Some studies suggest that GAGs act as crosslinkers between the collagen fibrils, while others have not found such evidence and postulate that GAGs have other functions.					
36827935	6	21	theme	chondroitinase	1167:1180	arg1	ABC					1182:1184	chondroitinase ABC	1167:1184	chondroitinase ABC	1167:1184	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	1	22	theme	collagen	190:197	arg1	fibrils					199:205	collagen fibrils	190:205	collagen fibrils	190:205	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	0	23	theme	indentation-based	3:19	arg1	framework					21:29	An indentation-based framework	0:29	An indentation-based framework for probing the glycosaminoglycan-mediated interactions of collagen fibrils	0:105	An indentation-based framework for probing the glycosaminoglycan-mediated interactions of collagen fibrils.					
36827935	7	24	theme	modeling	1491:1498	arg1	approach					1500:1507	the modeling approach	1487:1507	the modeling approach	1487:1507	We validate the modeling approach experimentally and show that GAGs facilitate collagen fibril sliding with minimal crosslinking function.					
36827935	6	25	theme	indentation	1265:1275	arg1	response					1277:1284	the orientation-dependent indentation response	1239:1284	the orientation-dependent indentation response in transverse and longitudinal orientations	1239:1328	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	1	26	theme	Microscale	108:117	arg1	reorientation					150:162	reorientation	150:162	reorientation	150:162	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	1	26	theme	Microscale	108:117	arg1	processes					131:139	Microscale deformation processes	108:139	Microscale deformation processes	108:139	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	1	26	theme	Microscale	108:117	arg1	buckling					165:172	buckling	165:172	buckling	165:172	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	1	26	theme	Microscale	108:117	arg1	sliding					179:185	sliding	179:185	sliding	179:185	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	1	27	theme	fibrils	199:205	arg1	reorientation					150:162	reorientation	150:162	reorientation	150:162	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	1	27	theme	fibrils	199:205	arg1	buckling					165:172	buckling	165:172	buckling	165:172	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	1	27	theme	fibrils	199:205	arg1	sliding					179:185	sliding	179:185	sliding	179:185	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	6	28	theme	orientation-dependent	1243:1263	arg1	response					1277:1284	the orientation-dependent indentation response	1239:1284	the orientation-dependent indentation response in transverse and longitudinal orientations	1239:1328	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	2	29	theme	tendon	328:333	arg1	structure					299:307	structure	299:307	structure	299:307	While changes in the structure and composition of tendon have been extensively studied, the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents are not well understood, partly due to the lack of appropriate techniques to probe the behavior.					
36827935	2	29	theme	tendon	328:333	arg1	composition					313:323	composition	313:323	composition	313:323	While changes in the structure and composition of tendon have been extensively studied, the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents are not well understood, partly due to the lack of appropriate techniques to probe the behavior.					
36827935	7	30	theme	fibril	1563:1568	arg1	sliding					1570:1576	collagen fibril sliding	1554:1576	collagen fibril sliding	1554:1576	We validate the modeling approach experimentally and show that GAGs facilitate collagen fibril sliding with minimal crosslinking function.					
36827935	5	31	theme	length	1084:1089	arg1	scale					1091:1095	a length scale	1082:1095	a length scale more relevant to fibrils compared to bulk tests	1082:1143	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	2	32	theme	constituents	453:464	arg1	interaction					411:421	the interaction	407:421	the interaction of extracellular matrix (ECM) constituents	407:464	While changes in the structure and composition of tendon have been extensively studied, the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents are not well understood, partly due to the lack of appropriate techniques to probe the behavior.					
36827935	5	33	theme	GAGs	1027:1030	arg1	function					1015:1022	the shear-mediated function	996:1022	the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests	996:1143	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	1	34	theme	deformation	119:129	arg1	reorientation					150:162	reorientation	150:162	reorientation	150:162	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	1	34	theme	deformation	119:129	arg1	processes					131:139	Microscale deformation processes	108:139	Microscale deformation processes	108:139	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	1	34	theme	deformation	119:129	arg1	buckling					165:172	buckling	165:172	buckling	165:172	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	1	34	theme	deformation	119:129	arg1	sliding					179:185	sliding	179:185	sliding	179:185	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	5	35	theme	tissue	949:954	arg1	behavior					937:944	orientation-dependent indentation behavior	903:944	orientation-dependent indentation behavior of tissue and computational modeling	903:981	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	7	36	theme	crosslinking	1591:1602	arg1	function					1604:1611	minimal crosslinking function	1583:1611	minimal crosslinking function	1583:1611	We validate the modeling approach experimentally and show that GAGs facilitate collagen fibril sliding with minimal crosslinking function.					
36827935	5	37	theme	computational	960:972	arg1	modeling					974:981	computational modeling	960:981	computational modeling	960:981	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	2	38	from	changes	284:290	arg1	structure					299:307	structure	299:307	structure	299:307	While changes in the structure and composition of tendon have been extensively studied, the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents are not well understood, partly due to the lack of appropriate techniques to probe the behavior.					
36827935	2	38	from	changes	284:290	arg1	composition					313:323	composition	313:323	composition	313:323	While changes in the structure and composition of tendon have been extensively studied, the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents are not well understood, partly due to the lack of appropriate techniques to probe the behavior.					
36827935	6	39	theme	shear-lag	1458:1466	arg1	model					1468:1472	a modified shear-lag model	1447:1472	a modified shear-lag model	1447:1472	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	6	40	theme	deformation	1356:1366	arg1	mechanisms					1368:1377	microscale deformation mechanisms	1345:1377	microscale deformation mechanisms	1345:1377	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	1	41	theme	mechanical	222:231	arg1	behavior					233:240	the mechanical behavior	218:240	the mechanical behavior	218:240	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
36827935	3	42	theme	glycosaminoglycans	590:607	arg1	role					582:585	the role	578:585	the role of glycosaminoglycans (GAGs) in modulating collagen fibril interactions	578:657	In particular, the role of glycosaminoglycans (GAGs) in modulating collagen fibril interactions has remained controversial.					
36827935	9	43	theme	collagenous	1976:1986	arg1	tissue					1988:1993	collagenous tissue	1976:1993	collagenous tissue	1976:1993	This study reveals the mechanisms that control the orientation-dependent indentation response by affecting the shear deformation and provides new insights into the mechanical function of GAGs and collagen crosslinkers in collagenous tissue.					
36827935	2	44	theme	matrix	440:445	arg1	constituents					453:464	extracellular matrix (ECM) constituents	426:464	extracellular matrix (ECM) constituents	426:464	While changes in the structure and composition of tendon have been extensively studied, the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents are not well understood, partly due to the lack of appropriate techniques to probe the behavior.					
36827935	6	45	theme	modified	1449:1456	arg1	model					1468:1472	a modified shear-lag model	1447:1472	a modified shear-lag model	1447:1472	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	8	46	theme	molecular	1634:1642	arg1	reconfiguration					1644:1658	the molecular reconfiguration	1630:1658	the molecular reconfiguration of GAGs	1630:1666	We suggest that the molecular reconfiguration of GAGs is a potential mechanism for their microscale, strain-dependent viscoelastic behavior.					
36827935	8	46	theme	molecular	1634:1642	arg1	mechanism					1683:1691	a potential mechanism	1671:1691	a potential mechanism for their microscale, strain-dependent viscoelastic behavior	1671:1752	We suggest that the molecular reconfiguration of GAGs is a potential mechanism for their microscale, strain-dependent viscoelastic behavior.					
36827935	6	47	theme	microscale	1345:1354	arg1	mechanisms					1368:1377	microscale deformation mechanisms	1345:1377	microscale deformation mechanisms	1345:1377	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	3	48	theme	collagen	630:637	arg1	interactions					646:657	collagen fibril interactions	630:657	collagen fibril interactions	630:657	In particular, the role of glycosaminoglycans (GAGs) in modulating collagen fibril interactions has remained controversial.					
36827935	2	49	theme	extracellular	426:438	arg1	matrix					440:445	extracellular matrix	426:445	extracellular matrix (ECM) constituents	426:464	While changes in the structure and composition of tendon have been extensively studied, the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents are not well understood, partly due to the lack of appropriate techniques to probe the behavior.					
36827935	2	49	theme	extracellular	426:438	arg1	ECM					448:450	ECM	448:450	ECM	448:450	While changes in the structure and composition of tendon have been extensively studied, the deformation mechanisms that modulate the interaction of extracellular matrix (ECM) constituents are not well understood, partly due to the lack of appropriate techniques to probe the behavior.					
36827935	9	50	theme	GAGs	1942:1945	arg1	function					1930:1937	the mechanical function	1915:1937	the mechanical function of GAGs and collagen crosslinkers in collagenous tissue	1915:1993	This study reveals the mechanisms that control the orientation-dependent indentation response by affecting the shear deformation and provides new insights into the mechanical function of GAGs and collagen crosslinkers in collagenous tissue.					
36827935	7	51	theme	minimal	1583:1589	arg1	function					1604:1611	minimal crosslinking function	1583:1611	minimal crosslinking function	1583:1611	We validate the modeling approach experimentally and show that GAGs facilitate collagen fibril sliding with minimal crosslinking function.					
36827935	3	52	theme	fibril	639:644	arg1	interactions					646:657	collagen fibril interactions	630:657	collagen fibril interactions	630:657	In particular, the role of glycosaminoglycans (GAGs) in modulating collagen fibril interactions has remained controversial.					
36827935	0	53	theme	glycosaminoglycan-mediated	47:72	arg1	interactions					74:85	the glycosaminoglycan-mediated interactions	43:85	the glycosaminoglycan-mediated interactions of collagen fibrils	43:105	An indentation-based framework for probing the glycosaminoglycan-mediated interactions of collagen fibrils.					
36827935	9	54	theme	orientation-dependent	1806:1826	arg1	response					1840:1847	the orientation-dependent indentation response	1802:1847	the orientation-dependent indentation response	1802:1847	This study reveals the mechanisms that control the orientation-dependent indentation response by affecting the shear deformation and provides new insights into the mechanical function of GAGs and collagen crosslinkers in collagenous tissue.					
36827935	5	55	theme	collagen	1050:1057	arg1	interactions					1066:1077	the collagen fibril interactions	1046:1077	the collagen fibril interactions	1046:1077	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	9	56	theme	new	1897:1899	arg1	insights					1901:1908	new insights	1897:1908	new insights into the mechanical function of GAGs and collagen crosslinkers in collagenous tissue	1897:1993	This study reveals the mechanisms that control the orientation-dependent indentation response by affecting the shear deformation and provides new insights into the mechanical function of GAGs and collagen crosslinkers in collagenous tissue.					
36827935	5	57	theme	orientation-dependent	903:923	arg1	behavior					937:944	orientation-dependent indentation behavior	903:944	orientation-dependent indentation behavior of tissue and computational modeling	903:981	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	9	58	theme	collagen	1951:1958	arg1	crosslinkers					1960:1971	collagen crosslinkers	1951:1971	collagen crosslinkers	1951:1971	This study reveals the mechanisms that control the orientation-dependent indentation response by affecting the shear deformation and provides new insights into the mechanical function of GAGs and collagen crosslinkers in collagenous tissue.					
36827935	9	59	theme	indentation	1828:1838	arg1	response					1840:1847	the orientation-dependent indentation response	1802:1847	the orientation-dependent indentation response	1802:1847	This study reveals the mechanisms that control the orientation-dependent indentation response by affecting the shear deformation and provides new insights into the mechanical function of GAGs and collagen crosslinkers in collagenous tissue.					
36827935	5	60	theme	fibril	1059:1064	arg1	interactions					1066:1077	the collagen fibril interactions	1046:1077	the collagen fibril interactions	1046:1077	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	4	61	theme	such	796:799	arg1	evidence					801:808	such evidence	796:808	such evidence	796:808	Some studies suggest that GAGs act as crosslinkers between the collagen fibrils, while others have not found such evidence and postulate that GAGs have other functions.					
36827935	5	62	theme	indentation	925:935	arg1	behavior					937:944	orientation-dependent indentation behavior	903:944	orientation-dependent indentation behavior of tissue and computational modeling	903:981	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	9	63	theme	crosslinkers	1960:1971	arg1	function					1930:1937	the mechanical function	1915:1937	the mechanical function of GAGs and collagen crosslinkers in collagenous tissue	1915:1993	This study reveals the mechanisms that control the orientation-dependent indentation response by affecting the shear deformation and provides new insights into the mechanical function of GAGs and collagen crosslinkers in collagenous tissue.					
36827935	5	64	theme	shear-mediated	1000:1013	arg1	function					1015:1022	the shear-mediated function	996:1022	the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests	996:1143	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	6	65	theme	GAGs	1395:1398	arg1	function					1383:1390	function	1383:1390	function	1383:1390	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	6	65	theme	GAGs	1395:1398	arg1	mechanisms					1368:1377	microscale deformation mechanisms	1345:1377	microscale deformation mechanisms	1345:1377	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	8	66	theme	GAGs	1663:1666	arg1	reconfiguration					1644:1658	the molecular reconfiguration	1630:1658	the molecular reconfiguration of GAGs	1630:1666	We suggest that the molecular reconfiguration of GAGs is a potential mechanism for their microscale, strain-dependent viscoelastic behavior.					
36827935	8	66	theme	GAGs	1663:1666	arg1	mechanism					1683:1691	a potential mechanism	1671:1691	a potential mechanism for their microscale, strain-dependent viscoelastic behavior	1671:1752	We suggest that the molecular reconfiguration of GAGs is a potential mechanism for their microscale, strain-dependent viscoelastic behavior.					
36827935	0	67	theme	collagen	90:97	arg1	fibrils					99:105	collagen fibrils	90:105	collagen fibrils	90:105	An indentation-based framework for probing the glycosaminoglycan-mediated interactions of collagen fibrils.					
36827935	7	68	theme	collagen	1554:1561	arg1	sliding					1570:1576	collagen fibril sliding	1554:1576	collagen fibril sliding	1554:1576	We validate the modeling approach experimentally and show that GAGs facilitate collagen fibril sliding with minimal crosslinking function.					
36827935	5	69	theme	new	877:879	arg1	framework					881:889	a new framework	875:889	a new framework	875:889	Here, we introduce a new framework, relying on orientation-dependent indentation behavior of tissue and computational modeling, to evaluate the shear-mediated function of GAGs in modulating the collagen fibril interactions at a length scale more relevant to fibrils compared to bulk tests.					
36827935	8	70	theme	viscoelastic	1732:1743	arg1	behavior					1745:1752	their microscale, strain-dependent viscoelastic behavior	1697:1752	their microscale, strain-dependent viscoelastic behavior	1697:1752	We suggest that the molecular reconfiguration of GAGs is a potential mechanism for their microscale, strain-dependent viscoelastic behavior.					
36827935	8	71	theme	potential	1673:1681	arg1	reconfiguration					1644:1658	the molecular reconfiguration	1630:1658	the molecular reconfiguration of GAGs	1630:1666	We suggest that the molecular reconfiguration of GAGs is a potential mechanism for their microscale, strain-dependent viscoelastic behavior.					
36827935	8	71	theme	potential	1673:1681	arg1	mechanism					1683:1691	a potential mechanism	1671:1691	a potential mechanism for their microscale, strain-dependent viscoelastic behavior	1671:1752	We suggest that the molecular reconfiguration of GAGs is a potential mechanism for their microscale, strain-dependent viscoelastic behavior.					
36827935	6	72	theme	computational	1423:1435	arg1	model					1437:1441	a microstructural computational model	1405:1441	a microstructural computational model	1405:1441	Specifically, we use chondroitinase ABC to enzymatically deplete the GAGs in tendon; measure the orientation-dependent indentation response in transverse and longitudinal orientations; and infer the microscale deformation mechanisms and function of GAGs from a microstructural computational model and a modified shear-lag model.					
36827935	8	73	dep	microscale	1703:1712	arg1	strain-dependent					1715:1730	strain-dependent	1715:1730	strain-dependent	1715:1730	We suggest that the molecular reconfiguration of GAGs is a potential mechanism for their microscale, strain-dependent viscoelastic behavior.					
36827935	9	74	theme	mechanical	1919:1928	arg1	function					1930:1937	the mechanical function	1915:1937	the mechanical function of GAGs and collagen crosslinkers in collagenous tissue	1915:1993	This study reveals the mechanisms that control the orientation-dependent indentation response by affecting the shear deformation and provides new insights into the mechanical function of GAGs and collagen crosslinkers in collagenous tissue.					
36827935	1	75	theme	collagenous	258:268	arg1	tissue					270:275	collagenous tissue	258:275	collagenous tissue	258:275	Microscale deformation processes, such as reorientation, buckling, and sliding of collagen fibrils, determine the mechanical behavior and function of collagenous tissue.					
37073891	4	0	theme	new	911:913	arg1	CHT-N3					931:936	CHT-N3	931:936	CHT-N3	931:936	This copolymer is prepared via a copper-catalyzed 1,3-dipolar Huisgen cycloaddition between propargylated PCL (PCL-yne) and a new azido-chitosan (CHT-N3 ).					
37073891	4	0	theme	new	911:913	arg1	azido-chitosan					915:928	a new azido-chitosan	909:928	a new azido-chitosan (CHT-N3 )	909:938	This copolymer is prepared via a copper-catalyzed 1,3-dipolar Huisgen cycloaddition between propargylated PCL (PCL-yne) and a new azido-chitosan (CHT-N3 ).					
37073891	3	1	with	structure	737:745	arg1	chain					763:767	a CHT main chain	752:767	a CHT main chain	752:767	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	3	1	with	structure	737:745	arg1	grafts					777:782	PCL grafts	773:782	PCL grafts	773:782	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	3	2	theme	CHT	754:756	arg1	chain					763:767	a CHT main chain	752:767	a CHT main chain	752:767	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	0	3	theme	Graft	63:67	arg1	Copolymer					69:77	Amphiphilic Graft Copolymer	51:77	Amphiphilic Graft Copolymer	51:77	Synthesis of a New Poly(ε-Caprolactone)-g-Chitosan Amphiphilic Graft Copolymer With a "Reverse" Structure.					
37073891	5	4	theme	amphiphilic	963:973	arg1	copolymer					975:983	an amphiphilic copolymer	960:983	an amphiphilic copolymer	960:983	In order to obtain an amphiphilic copolymer regardless of the pH, chitosan oligomers, soluble at any pH, have been prepared and used.					
37073891	3	5	theme	main	758:761	arg1	chain					763:767	a CHT main chain	752:767	a CHT main chain	752:767	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	4	6	theme	1,3-dipolar	835:845	arg1	cycloaddition					855:867	a copper-catalyzed 1,3-dipolar Huisgen cycloaddition	816:867	a copper-catalyzed 1,3-dipolar Huisgen cycloaddition between propargylated PCL (PCL-yne) and a new azido-chitosan (CHT-N3 )	816:938	This copolymer is prepared via a copper-catalyzed 1,3-dipolar Huisgen cycloaddition between propargylated PCL (PCL-yne) and a new azido-chitosan (CHT-N3 ).					
37073891	3	7	theme	reverse	645:651	arg1	structure					654:662	an unusual "reverse" structure	633:662	an unusual "reverse" structure formed by a PCL backbone with CHT grafts	633:703	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	5	8	dep	obtain	953:958	arg1	to					950:951	to	950:951	to	950:951	In order to obtain an amphiphilic copolymer regardless of the pH, chitosan oligomers, soluble at any pH, have been prepared and used.					
37073891	4	9	theme	propargylated	877:889	arg1	PCL-yne					896:902	PCL-yne	896:902	PCL-yne	896:902	This copolymer is prepared via a copper-catalyzed 1,3-dipolar Huisgen cycloaddition between propargylated PCL (PCL-yne) and a new azido-chitosan (CHT-N3 ).					
37073891	4	9	theme	propargylated	877:889	arg1	PCL					891:893	propargylated PCL	877:893	propargylated PCL (PCL-yne)	877:903	This copolymer is prepared via a copper-catalyzed 1,3-dipolar Huisgen cycloaddition between propargylated PCL (PCL-yne) and a new azido-chitosan (CHT-N3 ).					
37073891	6	10	theme	hydrophobic	1189:1199	arg1	drugs					1201:1205	hydrophobic drugs	1189:1205	hydrophobic drugs	1189:1205	The amphiphilic PCL-g-CHT copolymer spontaneously self-assembles in water into nano-micelles that may incorporate hydrophobic drugs to give novel drug delivery systems.					
37073891	3	11	theme	new	513:515	arg1	copolymer					523:531	a new graft copolymer	511:531	a new graft copolymer	511:531	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	1	12	theme	many	242:245	arg1	applications					247:258	many applications	242:258	many applications	242:258	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	0	13	theme	Reverse	87:93	arg1	Structure					96:104	a "Reverse" Structure	84:104	a "Reverse" Structure	84:104	Synthesis of a New Poly(ε-Caprolactone)-g-Chitosan Amphiphilic Graft Copolymer With a "Reverse" Structure.					
37073891	3	14	theme	PCL	676:678	arg1	backbone					680:687	a PCL backbone	674:687	a PCL backbone with CHT grafts	674:703	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	6	15	theme	delivery	1226:1233	arg1	systems					1235:1241	novel drug delivery systems	1215:1241	novel drug delivery systems	1215:1241	The amphiphilic PCL-g-CHT copolymer spontaneously self-assembles in water into nano-micelles that may incorporate hydrophobic drugs to give novel drug delivery systems.					
37073891	3	16	with	backbone	680:687	arg1	grafts					698:703	CHT grafts	694:703	CHT grafts	694:703	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	1	17	theme	hydrophobic	138:148	arg1	PCL					171:173	PCL	171:173	PCL	171:173	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	17	theme	hydrophobic	138:148	arg1	ε-caprolactone					155:168	hydrophobic Poly ε-caprolactone	138:168	hydrophobic Poly ε-caprolactone (PCL)	138:174	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	17	theme	hydrophobic	138:148	arg1	Chitosan					119:126	Hydrophilic Chitosan	107:126	Hydrophilic Chitosan (CHT)	107:132	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	17	theme	hydrophobic	138:148	arg1	polymers					223:230	well-known biocompatible and biodegradable polymers	180:230	well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields	180:302	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	5	18	theme	chitosan	1007:1014	arg1	oligomers					1016:1024	chitosan oligomers	1007:1024	chitosan oligomers	1007:1024	In order to obtain an amphiphilic copolymer regardless of the pH, chitosan oligomers, soluble at any pH, have been prepared and used.					
37073891	1	19	theme	Poly	150:153	arg1	PCL					171:173	PCL	171:173	PCL	171:173	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	19	theme	Poly	150:153	arg1	ε-caprolactone					155:168	hydrophobic Poly ε-caprolactone	138:168	hydrophobic Poly ε-caprolactone (PCL)	138:174	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	19	theme	Poly	150:153	arg1	Chitosan					119:126	Hydrophilic Chitosan	107:126	Hydrophilic Chitosan (CHT)	107:132	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	19	theme	Poly	150:153	arg1	polymers					223:230	well-known biocompatible and biodegradable polymers	180:230	well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields	180:302	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	3	20	theme	CHT	694:696	arg1	grafts					698:703	CHT grafts	694:703	CHT grafts	694:703	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	0	21	theme	New	15:17	arg1	ε-Caprolactone					24:37	ε-Caprolactone	24:37	ε-Caprolactone	24:37	Synthesis of a New Poly(ε-Caprolactone)-g-Chitosan Amphiphilic Graft Copolymer With a "Reverse" Structure.					
37073891	0	21	theme	New	15:17	arg1	Poly					19:22	a New Poly	13:22	a New Poly(ε-Caprolactone)	13:38	Synthesis of a New Poly(ε-Caprolactone)-g-Chitosan Amphiphilic Graft Copolymer With a "Reverse" Structure.					
37073891	4	22	theme	Huisgen	847:853	arg1	cycloaddition					855:867	a copper-catalyzed 1,3-dipolar Huisgen cycloaddition	816:867	a copper-catalyzed 1,3-dipolar Huisgen cycloaddition between propargylated PCL (PCL-yne) and a new azido-chitosan (CHT-N3 )	816:938	This copolymer is prepared via a copper-catalyzed 1,3-dipolar Huisgen cycloaddition between propargylated PCL (PCL-yne) and a new azido-chitosan (CHT-N3 ).					
37073891	3	23	theme	graft	517:521	arg1	copolymer					523:531	a new graft copolymer	511:531	a new graft copolymer	511:531	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	0	24	theme	"	94:94	arg1	Structure					96:104	a "Reverse" Structure	84:104	a "Reverse" Structure	84:104	Synthesis of a New Poly(ε-Caprolactone)-g-Chitosan Amphiphilic Graft Copolymer With a "Reverse" Structure.					
37073891	3	25	theme	copolymer	523:531	arg1	synthesis					498:506	the synthesis	494:506	the synthesis of a new graft copolymer	494:531	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	1	26	theme	biomedical	267:276	arg1	fields					297:302	the biomedical and pharmaceutical fields	263:302	the biomedical and pharmaceutical fields	263:302	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	3	27	theme	amphiphilic	558:568	arg1	ε-caprolactone-g-Chitosan					575:599	ε-caprolactone-g-Chitosan	575:599	ε-caprolactone-g-Chitosan	575:599	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	3	27	theme	amphiphilic	558:568	arg1	PCL-g-CHT					603:611	PCL-g-CHT	603:611	PCL-g-CHT	603:611	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	3	27	theme	amphiphilic	558:568	arg1	Poly					570:573	the fully biodegradable amphiphilic Poly	534:573	the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT)	534:612	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	0	28	theme	Poly	19:22	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of a New Poly(ε-Caprolactone)	0:38	Synthesis of a New Poly(ε-Caprolactone)-g-Chitosan Amphiphilic Graft Copolymer With a "Reverse" Structure.					
37073891	5	29	from	pH	1042:1043	arg1	soluble					1027:1033	soluble	1027:1033	soluble	1027:1033	In order to obtain an amphiphilic copolymer regardless of the pH, chitosan oligomers, soluble at any pH, have been prepared and used.					
37073891	1	30	theme	pharmaceutical	282:295	arg1	fields					297:302	the biomedical and pharmaceutical fields	263:302	the biomedical and pharmaceutical fields	263:302	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	6	31	theme	drug	1221:1224	arg1	systems					1235:1241	novel drug delivery systems	1215:1241	novel drug delivery systems	1215:1241	The amphiphilic PCL-g-CHT copolymer spontaneously self-assembles in water into nano-micelles that may incorporate hydrophobic drugs to give novel drug delivery systems.					
37073891	2	32	theme	compounds	335:343	arg1	mixtures					313:320	the mixtures	309:320	the mixtures of these two compounds	309:343	But the mixtures of these two compounds are considered incompatible, which makes them not very interesting.					
37073891	5	33	theme	soluble	1027:1033	arg1	oligomers					1016:1024	chitosan oligomers	1007:1024	chitosan oligomers	1007:1024	In order to obtain an amphiphilic copolymer regardless of the pH, chitosan oligomers, soluble at any pH, have been prepared and used.					
37073891	3	34	theme	biodegradable	544:556	arg1	ε-caprolactone-g-Chitosan					575:599	ε-caprolactone-g-Chitosan	575:599	ε-caprolactone-g-Chitosan	575:599	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	3	34	theme	biodegradable	544:556	arg1	PCL-g-CHT					603:611	PCL-g-CHT	603:611	PCL-g-CHT	603:611	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	3	34	theme	biodegradable	544:556	arg1	Poly					570:573	the fully biodegradable amphiphilic Poly	534:573	the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT)	534:612	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	3	35	theme	PCL	773:775	arg1	grafts					777:782	PCL grafts	773:782	PCL grafts	773:782	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	6	36	theme	amphiphilic	1079:1089	arg1	copolymer					1101:1109	The amphiphilic PCL-g-CHT copolymer	1075:1109	The amphiphilic PCL-g-CHT copolymer	1075:1109	The amphiphilic PCL-g-CHT copolymer spontaneously self-assembles in water into nano-micelles that may incorporate hydrophobic drugs to give novel drug delivery systems.					
37073891	4	37	theme	copper-catalyzed	818:833	arg1	cycloaddition					855:867	a copper-catalyzed 1,3-dipolar Huisgen cycloaddition	816:867	a copper-catalyzed 1,3-dipolar Huisgen cycloaddition between propargylated PCL (PCL-yne) and a new azido-chitosan (CHT-N3 )	816:938	This copolymer is prepared via a copper-catalyzed 1,3-dipolar Huisgen cycloaddition between propargylated PCL (PCL-yne) and a new azido-chitosan (CHT-N3 ).					
37073891	3	38	theme	unusual	636:642	arg1	structure					654:662	an unusual "reverse" structure	633:662	an unusual "reverse" structure formed by a PCL backbone with CHT grafts	633:703	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	3	39	theme	CHT-g-PCL	727:735	arg1	structure					737:745	the "classic" CHT-g-PCL structure	713:745	the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts	713:782	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	6	40	theme	novel	1215:1219	arg1	systems					1235:1241	novel drug delivery systems	1215:1241	novel drug delivery systems	1215:1241	The amphiphilic PCL-g-CHT copolymer spontaneously self-assembles in water into nano-micelles that may incorporate hydrophobic drugs to give novel drug delivery systems.					
37073891	5	41	used	used	1069:1072	arg2	oligomers					1016:1024	chitosan oligomers	1007:1024	chitosan oligomers	1007:1024	In order to obtain an amphiphilic copolymer regardless of the pH, chitosan oligomers, soluble at any pH, have been prepared and used.					
37073891	1	42	contain	have	237:240	arg2	applications					247:258	many applications	242:258	many applications	242:258	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	42	contain	have	237:240	arg1	ε-caprolactone					155:168	hydrophobic Poly ε-caprolactone	138:168	hydrophobic Poly ε-caprolactone (PCL)	138:174	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	42	contain	have	237:240	arg1	Chitosan					119:126	Hydrophilic Chitosan	107:126	Hydrophilic Chitosan (CHT)	107:132	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	42	contain	have	237:240	arg1	polymers					223:230	well-known biocompatible and biodegradable polymers	180:230	well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields	180:302	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	43	theme	Hydrophilic	107:117	arg1	ε-caprolactone					155:168	hydrophobic Poly ε-caprolactone	138:168	hydrophobic Poly ε-caprolactone (PCL)	138:174	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	43	theme	Hydrophilic	107:117	arg1	CHT					129:131	CHT	129:131	CHT	129:131	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	43	theme	Hydrophilic	107:117	arg1	polymers					223:230	well-known biocompatible and biodegradable polymers	180:230	well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields	180:302	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	43	theme	Hydrophilic	107:117	arg1	Chitosan					119:126	Hydrophilic Chitosan	107:126	Hydrophilic Chitosan (CHT)	107:132	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	44	theme	biocompatible	191:203	arg1	ε-caprolactone					155:168	hydrophobic Poly ε-caprolactone	138:168	hydrophobic Poly ε-caprolactone (PCL)	138:174	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	44	theme	biocompatible	191:203	arg1	Chitosan					119:126	Hydrophilic Chitosan	107:126	Hydrophilic Chitosan (CHT)	107:132	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	44	theme	biocompatible	191:203	arg1	polymers					223:230	well-known biocompatible and biodegradable polymers	180:230	well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields	180:302	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	0	45	theme	Amphiphilic	51:61	arg1	Copolymer					69:77	Amphiphilic Graft Copolymer	51:77	Amphiphilic Graft Copolymer	51:77	Synthesis of a New Poly(ε-Caprolactone)-g-Chitosan Amphiphilic Graft Copolymer With a "Reverse" Structure.					
37073891	6	46	theme	PCL-g-CHT	1091:1099	arg1	copolymer					1101:1109	The amphiphilic PCL-g-CHT copolymer	1075:1109	The amphiphilic PCL-g-CHT copolymer	1075:1109	The amphiphilic PCL-g-CHT copolymer spontaneously self-assembles in water into nano-micelles that may incorporate hydrophobic drugs to give novel drug delivery systems.					
37073891	3	47	theme	homopolymers	481:492	arg1	properties					461:470	the properties	457:470	the properties of these homopolymers	457:492	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	3	48	theme	classic	718:724	arg1	structure					737:745	the "classic" CHT-g-PCL structure	713:745	the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts	713:782	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	3	49	theme	"	652:652	arg1	structure					654:662	an unusual "reverse" structure	633:662	an unusual "reverse" structure formed by a PCL backbone with CHT grafts	633:703	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
37073891	1	50	theme	biodegradable	209:221	arg1	ε-caprolactone					155:168	hydrophobic Poly ε-caprolactone	138:168	hydrophobic Poly ε-caprolactone (PCL)	138:174	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	50	theme	biodegradable	209:221	arg1	Chitosan					119:126	Hydrophilic Chitosan	107:126	Hydrophilic Chitosan (CHT)	107:132	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	50	theme	biodegradable	209:221	arg1	polymers					223:230	well-known biocompatible and biodegradable polymers	180:230	well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields	180:302	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	51	theme	well-known	180:189	arg1	ε-caprolactone					155:168	hydrophobic Poly ε-caprolactone	138:168	hydrophobic Poly ε-caprolactone (PCL)	138:174	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	51	theme	well-known	180:189	arg1	Chitosan					119:126	Hydrophilic Chitosan	107:126	Hydrophilic Chitosan (CHT)	107:132	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	1	51	theme	well-known	180:189	arg1	polymers					223:230	well-known biocompatible and biodegradable polymers	180:230	well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields	180:302	Hydrophilic Chitosan (CHT) and hydrophobic Poly ε-caprolactone (PCL) are well-known biocompatible and biodegradable polymers that have many applications in the biomedical and pharmaceutical fields.					
37073891	3	52	theme	"	725:725	arg1	structure					737:745	the "classic" CHT-g-PCL structure	713:745	the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts	713:782	To avoid this problem and to further extend the properties of these homopolymers the synthesis of a new graft copolymer, the fully biodegradable amphiphilic Poly(ε-caprolactone-g-Chitosan) (PCL-g-CHT) is described, with an unusual "reverse" structure formed by a PCL backbone with CHT grafts, unlike the "classic" CHT-g-PCL structure with a CHT main chain and PCL grafts.					
36304461	9	0	with	crosstalk	2132:2140	arg1	cells					2163:2167	host epithelial cells	2147:2167	host epithelial cells	2147:2167	These findings indicate that the secretion of OMVs by B. fragilis may facilitate immune crosstalk with host epithelial cells at the gastrointestinal surface and suggests that OMVs produced by commensal bacteria may preferentially activate host innate immune receptors at the mucosal gastrointestinal tract.					
36304461	9	1	theme	immune	2125:2130	arg1	crosstalk					2132:2140	immune crosstalk	2125:2140	immune crosstalk with host epithelial cells	2125:2167	These findings indicate that the secretion of OMVs by B. fragilis may facilitate immune crosstalk with host epithelial cells at the gastrointestinal surface and suggests that OMVs produced by commensal bacteria may preferentially activate host innate immune receptors at the mucosal gastrointestinal tract.					
36304461	2	2	theme	membrane	342:349	arg1	vesicles					351:358	Outer membrane vesicles	336:358	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria	336:400	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria can harbor various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan, and the composition of OMVs strongly influences their ability to activate host innate immune receptors.					
36304461	2	2	theme	membrane	342:349	arg1	OMVs					361:364	OMVs	361:364	OMVs	361:364	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria can harbor various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan, and the composition of OMVs strongly influences their ability to activate host innate immune receptors.					
36304461	1	3	theme	commensal	247:255	arg1	bacteria					257:264	both pathogenic and commensal bacteria	227:264	both pathogenic and commensal bacteria	227:264	The release of bacterial membrane vesicles (BMVs) has become recognized as a key mechanism used by both pathogenic and commensal bacteria to activate innate immune responses in the host and mediate immunity.					
36304461	8	4	theme	innate	1863:1868	arg1	receptors					1877:1885	host innate immune receptors	1858:1885	host innate immune receptors	1858:1885	Overall, our data demonstrates that B. fragilis OMVs activate a broader range of host innate immune receptors compared to their parent bacteria due to their enrichment of biological cargo and their ability to transport this cargo directly into host epithelial cells.					
36304461	9	5	theme	epithelial	2152:2161	arg1	cells					2163:2167	host epithelial cells	2147:2167	host epithelial cells	2147:2167	These findings indicate that the secretion of OMVs by B. fragilis may facilitate immune crosstalk with host epithelial cells at the gastrointestinal surface and suggests that OMVs produced by commensal bacteria may preferentially activate host innate immune receptors at the mucosal gastrointestinal tract.					
36304461	8	6	theme	receptors	1877:1885	arg1	range					1849:1853	a broader range	1839:1853	a broader range of host innate immune receptors	1839:1885	Overall, our data demonstrates that B. fragilis OMVs activate a broader range of host innate immune receptors compared to their parent bacteria due to their enrichment of biological cargo and their ability to transport this cargo directly into host epithelial cells.					
36304461	9	7	theme	gastrointestinal	2176:2191	arg1	surface					2193:2199	the gastrointestinal surface	2172:2199	the gastrointestinal surface	2172:2199	These findings indicate that the secretion of OMVs by B. fragilis may facilitate immune crosstalk with host epithelial cells at the gastrointestinal surface and suggests that OMVs produced by commensal bacteria may preferentially activate host innate immune receptors at the mucosal gastrointestinal tract.					
36304461	6	8	theme	intestinal	1342:1351	arg1	cells					1364:1368	intestinal epithelial cells	1342:1368	intestinal epithelial cells	1342:1368	We visualized the entry of B. fragilis OMVs into intestinal epithelial cells, in addition to the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells.					
36304461	6	9	theme	OMVs	1332:1335	arg1	entry					1311:1315	the entry	1307:1315	the entry of B. fragilis OMVs into intestinal epithelial cells	1307:1368	We visualized the entry of B. fragilis OMVs into intestinal epithelial cells, in addition to the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells.					
36304461	5	10	theme	various	1112:1118	arg1	RNA					1162:1164	RNA	1162:1164	RNA	1162:1164	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	5	10	theme	various	1112:1118	arg1	peptidoglycan					1197:1209	peptidoglycan	1197:1209	peptidoglycan	1197:1209	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	5	10	theme	various	1112:1118	arg1	cargo					1131:1135	various biological cargo	1112:1135	various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan	1112:1209	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	5	10	theme	various	1112:1118	arg1	proteins					1147:1154	proteins	1147:1154	proteins	1147:1154	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	5	10	theme	various	1112:1118	arg1	lipopolysaccharides					1167:1185	lipopolysaccharides	1167:1185	lipopolysaccharides (LPS)	1167:1191	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	5	10	theme	various	1112:1118	arg1	DNA					1157:1159	DNA	1157:1159	DNA	1157:1159	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	6	11	theme	bacterial	1431:1439	arg1	RNA					1441:1443	bacterial RNA and peptidoglycan cargo	1431:1467	RNA	1441:1443	We visualized the entry of B. fragilis OMVs into intestinal epithelial cells, in addition to the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells.					
36304461	2	12	theme	innate	575:580	arg1	receptors					589:597	host innate immune receptors	570:597	host innate immune receptors	570:597	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria can harbor various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan, and the composition of OMVs strongly influences their ability to activate host innate immune receptors.					
36304461	3	13	theme	commensal	764:772	arg1	organisms					774:782	commensal organisms	764:782	commensal organisms	764:782	Although various Gram-negative pathogens can produce OMVs that are enriched in immunogenic cargo compared to their parent bacteria, the ability of OMVs produced by commensal organisms to be enriched with immunostimulatory contents is only recently becoming known.					
36304461	5	14	theme	B. fragilis	1067:1077	arg1	OMVs					1079:1082	B. fragilis OMVs	1067:1082	B. fragilis OMVs	1067:1082	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	3	15	theme	immunostimulatory	804:820	arg1	contents					822:829	immunostimulatory contents	804:829	immunostimulatory contents	804:829	Although various Gram-negative pathogens can produce OMVs that are enriched in immunogenic cargo compared to their parent bacteria, the ability of OMVs produced by commensal organisms to be enriched with immunostimulatory contents is only recently becoming known.					
36304461	1	16	theme	bacterial	143:151	arg1	vesicles					162:169	bacterial membrane vesicles	143:169	bacterial membrane vesicles (BMVs)	143:176	The release of bacterial membrane vesicles (BMVs) has become recognized as a key mechanism used by both pathogenic and commensal bacteria to activate innate immune responses in the host and mediate immunity.					
36304461	1	16	theme	bacterial	143:151	arg1	BMVs					172:175	BMVs	172:175	BMVs	172:175	The release of bacterial membrane vesicles (BMVs) has become recognized as a key mechanism used by both pathogenic and commensal bacteria to activate innate immune responses in the host and mediate immunity.					
36304461	9	17	theme	commensal	2236:2244	arg1	bacteria					2246:2253	commensal bacteria	2236:2253	commensal bacteria	2236:2253	These findings indicate that the secretion of OMVs by B. fragilis may facilitate immune crosstalk with host epithelial cells at the gastrointestinal surface and suggests that OMVs produced by commensal bacteria may preferentially activate host innate immune receptors at the mucosal gastrointestinal tract.					
36304461	8	18	theme	cargo	1959:1963	arg1	enrichment					1934:1943	their enrichment	1928:1943	their enrichment of biological cargo	1928:1963	Overall, our data demonstrates that B. fragilis OMVs activate a broader range of host innate immune receptors compared to their parent bacteria due to their enrichment of biological cargo and their ability to transport this cargo directly into host epithelial cells.					
36304461	0	19	theme	outer	21:25	arg1	vesicles					36:43	Bacteroides fragilis outer membrane vesicles	0:43	Bacteroides fragilis outer membrane vesicles	0:43	Bacteroides fragilis outer membrane vesicles preferentially activate innate immune receptors compared to their parent bacteria.					
36304461	1	20	theme	vesicles	162:169	arg1	release					132:138	The release	128:138	The release of bacterial membrane vesicles (BMVs)	128:176	The release of bacterial membrane vesicles (BMVs) has become recognized as a key mechanism used by both pathogenic and commensal bacteria to activate innate immune responses in the host and mediate immunity.					
36304461	7	21	theme	B. fragilis	1714:1724	arg1	bacteria					1726:1733	B. fragilis bacteria	1714:1733	B. fragilis bacteria	1714:1733	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	22	theme	Toll-like	1591:1599	arg1	TLR7					1626:1629	TLR7	1626:1629	TLR7	1626:1629	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	22	theme	Toll-like	1591:1599	arg1	TLR					1612:1614	TLR	1612:1614	TLR	1612:1614	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	22	theme	Toll-like	1591:1599	arg1	protein					1688:1694	nucleotide-binding oligomerization domain-containing protein 1	1635:1696	nucleotide-binding oligomerization domain-containing protein 1	1635:1696	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	22	theme	Toll-like	1591:1599	arg1	receptors					1601:1609	host Toll-like receptors	1586:1609	host Toll-like receptors (TLR)	1586:1615	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	22	theme	Toll-like	1591:1599	arg1	TLR4					1620:1623	TLR4	1620:1623	TLR4	1620:1623	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	4	23	theme	commensal	953:961	arg1	Bacteroides fragilis					963:982	the intestinal commensal Bacteroides fragilis	938:982	the intestinal commensal Bacteroides fragilis	938:982	In this study, we investigated the cargo associated with OMVs produced by the intestinal commensal Bacteroides fragilis and determined their ability to activate host innate immune receptors.					
36304461	5	24	theme	parent	1276:1281	arg1	bacteria					1283:1290	their parent bacteria	1270:1290	their parent bacteria	1270:1290	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	2	25	theme	Gram-negative	379:391	arg1	bacteria					393:400	Gram-negative bacteria	379:400	Gram-negative bacteria	379:400	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria can harbor various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan, and the composition of OMVs strongly influences their ability to activate host innate immune receptors.					
36304461	8	26	theme	epithelial	2026:2035	arg1	cells					2037:2041	host epithelial cells	2021:2041	host epithelial cells	2021:2041	Overall, our data demonstrates that B. fragilis OMVs activate a broader range of host innate immune receptors compared to their parent bacteria due to their enrichment of biological cargo and their ability to transport this cargo directly into host epithelial cells.					
36304461	3	27	dep	becoming	848:855	arg1	known					857:861	known	857:861	is only recently becoming known	831:861	Although various Gram-negative pathogens can produce OMVs that are enriched in immunogenic cargo compared to their parent bacteria, the ability of OMVs produced by commensal organisms to be enriched with immunostimulatory contents is only recently becoming known.					
36304461	0	28	theme	Bacteroides	0:10	arg1	vesicles					36:43	Bacteroides fragilis outer membrane vesicles	0:43	Bacteroides fragilis outer membrane vesicles	0:43	Bacteroides fragilis outer membrane vesicles preferentially activate innate immune receptors compared to their parent bacteria.					
36304461	6	29	dep	ability	1390:1396	arg1	addition					1374:1381	addition	1374:1381	addition	1374:1381	We visualized the entry of B. fragilis OMVs into intestinal epithelial cells, in addition to the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells.					
36304461	7	30	theme	domain-containing	1670:1686	arg1	protein					1688:1694	nucleotide-binding oligomerization domain-containing protein 1	1635:1696	nucleotide-binding oligomerization domain-containing protein 1	1635:1696	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	30	theme	domain-containing	1670:1686	arg1	receptors					1601:1609	host Toll-like receptors	1586:1609	host Toll-like receptors (TLR)	1586:1615	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	2	31	theme	various	413:419	arg1	cargo					433:437	various immunogenic cargo	413:437	various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan	413:493	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria can harbor various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan, and the composition of OMVs strongly influences their ability to activate host innate immune receptors.					
36304461	8	32	theme	host	2021:2024	arg1	cells					2037:2041	host epithelial cells	2021:2041	host epithelial cells	2021:2041	Overall, our data demonstrates that B. fragilis OMVs activate a broader range of host innate immune receptors compared to their parent bacteria due to their enrichment of biological cargo and their ability to transport this cargo directly into host epithelial cells.					
36304461	1	33	theme	immune	285:290	arg1	responses					292:300	innate immune responses	278:300	innate immune responses	278:300	The release of bacterial membrane vesicles (BMVs) has become recognized as a key mechanism used by both pathogenic and commensal bacteria to activate innate immune responses in the host and mediate immunity.					
36304461	6	34	dep	B.	1401:1402	arg1	fragilis					1404:1411	fragilis	1404:1411	fragilis	1404:1411	We visualized the entry of B. fragilis OMVs into intestinal epithelial cells, in addition to the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells.					
36304461	7	35	theme	nucleotide-binding	1635:1652	arg1	protein					1688:1694	nucleotide-binding oligomerization domain-containing protein 1	1635:1696	nucleotide-binding oligomerization domain-containing protein 1	1635:1696	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	35	theme	nucleotide-binding	1635:1652	arg1	receptors					1601:1609	host Toll-like receptors	1586:1609	host Toll-like receptors (TLR)	1586:1615	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	4	36	theme	host	1025:1028	arg1	receptors					1044:1052	host innate immune receptors	1025:1052	host innate immune receptors	1025:1052	In this study, we investigated the cargo associated with OMVs produced by the intestinal commensal Bacteroides fragilis and determined their ability to activate host innate immune receptors.					
36304461	9	37	theme	gastrointestinal	2327:2342	arg1	tract					2344:2348	the mucosal gastrointestinal tract	2315:2348	the mucosal gastrointestinal tract	2315:2348	These findings indicate that the secretion of OMVs by B. fragilis may facilitate immune crosstalk with host epithelial cells at the gastrointestinal surface and suggests that OMVs produced by commensal bacteria may preferentially activate host innate immune receptors at the mucosal gastrointestinal tract.					
36304461	6	38	theme	OMVs	1413:1416	arg1	ability					1390:1396	the ability	1386:1396	the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells	1386:1496	We visualized the entry of B. fragilis OMVs into intestinal epithelial cells, in addition to the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells.					
36304461	4	39	theme	immune	1037:1042	arg1	receptors					1044:1052	host innate immune receptors	1025:1052	host innate immune receptors	1025:1052	In this study, we investigated the cargo associated with OMVs produced by the intestinal commensal Bacteroides fragilis and determined their ability to activate host innate immune receptors.					
36304461	0	40	theme	immune	76:81	arg1	receptors					83:91	innate immune receptors	69:91	innate immune receptors	69:91	Bacteroides fragilis outer membrane vesicles preferentially activate innate immune receptors compared to their parent bacteria.					
36304461	2	41	theme	Outer	336:340	arg1	vesicles					351:358	Outer membrane vesicles	336:358	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria	336:400	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria can harbor various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan, and the composition of OMVs strongly influences their ability to activate host innate immune receptors.					
36304461	2	41	theme	Outer	336:340	arg1	OMVs					361:364	OMVs	361:364	OMVs	361:364	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria can harbor various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan, and the composition of OMVs strongly influences their ability to activate host innate immune receptors.					
36304461	6	42	theme	B.	1401:1402	arg1	OMVs					1413:1416	B. fragilis OMVs	1401:1416	B. fragilis OMVs	1401:1416	We visualized the entry of B. fragilis OMVs into intestinal epithelial cells, in addition to the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells.					
36304461	7	43	theme	cell	1523:1526	arg1	lines					1528:1532	HEK-Blue reporter cell lines	1505:1532	HEK-Blue reporter cell lines	1505:1532	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	3	44	theme	Gram-negative	617:629	arg1	pathogens					631:639	various Gram-negative pathogens	609:639	various Gram-negative pathogens	609:639	Although various Gram-negative pathogens can produce OMVs that are enriched in immunogenic cargo compared to their parent bacteria, the ability of OMVs produced by commensal organisms to be enriched with immunostimulatory contents is only recently becoming known.					
36304461	9	45	theme	OMVs	2090:2093	arg1	secretion					2077:2085	the secretion	2073:2085	the secretion of OMVs by B. fragilis	2073:2108	These findings indicate that the secretion of OMVs by B. fragilis may facilitate immune crosstalk with host epithelial cells at the gastrointestinal surface and suggests that OMVs produced by commensal bacteria may preferentially activate host innate immune receptors at the mucosal gastrointestinal tract.					
36304461	9	46	theme	innate	2288:2293	arg1	receptors					2302:2310	host innate immune receptors	2283:2310	host innate immune receptors	2283:2310	These findings indicate that the secretion of OMVs by B. fragilis may facilitate immune crosstalk with host epithelial cells at the gastrointestinal surface and suggests that OMVs produced by commensal bacteria may preferentially activate host innate immune receptors at the mucosal gastrointestinal tract.					
36304461	7	47	theme	HEK-Blue	1505:1512	arg1	lines					1528:1532	HEK-Blue reporter cell lines	1505:1532	HEK-Blue reporter cell lines	1505:1532	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	6	48	theme	epithelial	1481:1490	arg1	cells					1492:1496	Caco-2 epithelial cells	1474:1496	Caco-2 epithelial cells	1474:1496	We visualized the entry of B. fragilis OMVs into intestinal epithelial cells, in addition to the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells.					
36304461	0	49	dep	Bacteroides	0:10	arg1	fragilis					12:19	fragilis	12:19	fragilis	12:19	Bacteroides fragilis outer membrane vesicles preferentially activate innate immune receptors compared to their parent bacteria.					
36304461	3	50	theme	parent	715:720	arg1	bacteria					722:729	their parent bacteria	709:729	their parent bacteria	709:729	Although various Gram-negative pathogens can produce OMVs that are enriched in immunogenic cargo compared to their parent bacteria, the ability of OMVs produced by commensal organisms to be enriched with immunostimulatory contents is only recently becoming known.					
36304461	8	51	theme	host	1858:1861	arg1	receptors					1877:1885	host innate immune receptors	1858:1885	host innate immune receptors	1858:1885	Overall, our data demonstrates that B. fragilis OMVs activate a broader range of host innate immune receptors compared to their parent bacteria due to their enrichment of biological cargo and their ability to transport this cargo directly into host epithelial cells.					
36304461	8	52	theme	immune	1870:1875	arg1	receptors					1877:1885	host innate immune receptors	1858:1885	host innate immune receptors	1858:1885	Overall, our data demonstrates that B. fragilis OMVs activate a broader range of host innate immune receptors compared to their parent bacteria due to their enrichment of biological cargo and their ability to transport this cargo directly into host epithelial cells.					
36304461	6	53	theme	peptidoglycan	1449:1461	arg1	cargo					1463:1467	bacterial RNA and peptidoglycan cargo	1431:1467	cargo	1463:1467	We visualized the entry of B. fragilis OMVs into intestinal epithelial cells, in addition to the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells.					
36304461	2	54	theme	immune	582:587	arg1	receptors					589:597	host innate immune receptors	570:597	host innate immune receptors	570:597	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria can harbor various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan, and the composition of OMVs strongly influences their ability to activate host innate immune receptors.					
36304461	9	55	theme	host	2147:2150	arg1	cells					2163:2167	host epithelial cells	2147:2167	host epithelial cells	2147:2167	These findings indicate that the secretion of OMVs by B. fragilis may facilitate immune crosstalk with host epithelial cells at the gastrointestinal surface and suggests that OMVs produced by commensal bacteria may preferentially activate host innate immune receptors at the mucosal gastrointestinal tract.					
36304461	7	56	theme	TLR2	1771:1774	arg1	activation					1757:1766	the activation	1753:1766	the activation of TLR2	1753:1774	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	6	57	theme	epithelial	1353:1362	arg1	cells					1364:1368	intestinal epithelial cells	1342:1368	intestinal epithelial cells	1342:1368	We visualized the entry of B. fragilis OMVs into intestinal epithelial cells, in addition to the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells.					
36304461	3	58	theme	OMVs	747:750	arg1	ability					736:742	the ability	732:742	the ability of OMVs produced by commensal organisms to be enriched with immunostimulatory contents	732:829	Although various Gram-negative pathogens can produce OMVs that are enriched in immunogenic cargo compared to their parent bacteria, the ability of OMVs produced by commensal organisms to be enriched with immunostimulatory contents is only recently becoming known.					
36304461	1	59	theme	key	205:207	arg1	mechanism					209:217	a key mechanism	203:217	a key mechanism used by both pathogenic and commensal bacteria to activate innate immune responses in the host and mediate immunity	203:333	The release of bacterial membrane vesicles (BMVs) has become recognized as a key mechanism used by both pathogenic and commensal bacteria to activate innate immune responses in the host and mediate immunity.					
36304461	2	60	theme	OMVs	519:522	arg1	composition					504:514	the composition	500:514	the composition of OMVs	500:522	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria can harbor various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan, and the composition of OMVs strongly influences their ability to activate host innate immune receptors.					
36304461	6	61	theme	B. fragilis	1320:1330	arg1	OMVs					1332:1335	B. fragilis OMVs	1320:1335	B. fragilis OMVs	1320:1335	We visualized the entry of B. fragilis OMVs into intestinal epithelial cells, in addition to the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells.					
36304461	5	62	theme	biological	1120:1129	arg1	RNA					1162:1164	RNA	1162:1164	RNA	1162:1164	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	5	62	theme	biological	1120:1129	arg1	peptidoglycan					1197:1209	peptidoglycan	1197:1209	peptidoglycan	1197:1209	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	5	62	theme	biological	1120:1129	arg1	cargo					1131:1135	various biological cargo	1112:1135	various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan	1112:1209	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	5	62	theme	biological	1120:1129	arg1	proteins					1147:1154	proteins	1147:1154	proteins	1147:1154	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	5	62	theme	biological	1120:1129	arg1	lipopolysaccharides					1167:1185	lipopolysaccharides	1167:1185	lipopolysaccharides (LPS)	1167:1191	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	5	62	theme	biological	1120:1129	arg1	DNA					1157:1159	DNA	1157:1159	DNA	1157:1159	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	2	63	theme	host	570:573	arg1	receptors					589:597	host innate immune receptors	570:597	host innate immune receptors	570:597	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria can harbor various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan, and the composition of OMVs strongly influences their ability to activate host innate immune receptors.					
36304461	8	64	theme	biological	1948:1957	arg1	cargo					1959:1963	biological cargo	1948:1963	biological cargo	1948:1963	Overall, our data demonstrates that B. fragilis OMVs activate a broader range of host innate immune receptors compared to their parent bacteria due to their enrichment of biological cargo and their ability to transport this cargo directly into host epithelial cells.					
36304461	5	65	theme	OMVs	1079:1082	arg1	Analysis					1055:1062	Analysis	1055:1062	Analysis of B. fragilis OMVs	1055:1082	Analysis of B. fragilis OMVs revealed that they packaged various biological cargo including proteins, DNA, RNA, lipopolysaccharides (LPS) and peptidoglycan, and that this cargo could be enriched in OMVs compared to their parent bacteria.					
36304461	7	66	dep	activate	1577:1584	arg1	whereas					1706:1712	whereas	1706:1712	whereas	1706:1712	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	3	67	theme	various	609:615	arg1	pathogens					631:639	various Gram-negative pathogens	609:639	various Gram-negative pathogens	609:639	Although various Gram-negative pathogens can produce OMVs that are enriched in immunogenic cargo compared to their parent bacteria, the ability of OMVs produced by commensal organisms to be enriched with immunostimulatory contents is only recently becoming known.					
36304461	1	68	theme	membrane	153:160	arg1	vesicles					162:169	bacterial membrane vesicles	143:169	bacterial membrane vesicles (BMVs)	143:176	The release of bacterial membrane vesicles (BMVs) has become recognized as a key mechanism used by both pathogenic and commensal bacteria to activate innate immune responses in the host and mediate immunity.					
36304461	1	68	theme	membrane	153:160	arg1	BMVs					172:175	BMVs	172:175	BMVs	172:175	The release of bacterial membrane vesicles (BMVs) has become recognized as a key mechanism used by both pathogenic and commensal bacteria to activate innate immune responses in the host and mediate immunity.					
36304461	7	69	theme	host	1586:1589	arg1	TLR7					1626:1629	TLR7	1626:1629	TLR7	1626:1629	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	69	theme	host	1586:1589	arg1	TLR					1612:1614	TLR	1612:1614	TLR	1612:1614	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	69	theme	host	1586:1589	arg1	protein					1688:1694	nucleotide-binding oligomerization domain-containing protein 1	1635:1696	nucleotide-binding oligomerization domain-containing protein 1	1635:1696	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	69	theme	host	1586:1589	arg1	receptors					1601:1609	host Toll-like receptors	1586:1609	host Toll-like receptors (TLR)	1586:1615	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	69	theme	host	1586:1589	arg1	TLR4					1620:1623	TLR4	1620:1623	TLR4	1620:1623	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	8	70	theme	parent	1905:1910	arg1	bacteria					1912:1919	their parent bacteria	1899:1919	their parent bacteria due to their enrichment of biological cargo	1899:1963	Overall, our data demonstrates that B. fragilis OMVs activate a broader range of host innate immune receptors compared to their parent bacteria due to their enrichment of biological cargo and their ability to transport this cargo directly into host epithelial cells.					
36304461	0	71	theme	parent	111:116	arg1	bacteria					118:125	their parent bacteria	105:125	their parent bacteria	105:125	Bacteroides fragilis outer membrane vesicles preferentially activate innate immune receptors compared to their parent bacteria.					
36304461	7	72	theme	B. fragilis	1554:1564	arg1	OMVs					1566:1569	B. fragilis OMVs	1554:1569	B. fragilis OMVs	1554:1569	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	4	73	theme	intestinal	942:951	arg1	Bacteroides fragilis					963:982	the intestinal commensal Bacteroides fragilis	938:982	the intestinal commensal Bacteroides fragilis	938:982	In this study, we investigated the cargo associated with OMVs produced by the intestinal commensal Bacteroides fragilis and determined their ability to activate host innate immune receptors.					
36304461	9	74	theme	host	2283:2286	arg1	receptors					2302:2310	host innate immune receptors	2283:2310	host innate immune receptors	2283:2310	These findings indicate that the secretion of OMVs by B. fragilis may facilitate immune crosstalk with host epithelial cells at the gastrointestinal surface and suggests that OMVs produced by commensal bacteria may preferentially activate host innate immune receptors at the mucosal gastrointestinal tract.					
36304461	8	75	theme	B. fragilis	1813:1823	arg1	OMVs					1825:1828	B. fragilis OMVs	1813:1828	B. fragilis OMVs	1813:1828	Overall, our data demonstrates that B. fragilis OMVs activate a broader range of host innate immune receptors compared to their parent bacteria due to their enrichment of biological cargo and their ability to transport this cargo directly into host epithelial cells.					
36304461	9	76	theme	immune	2295:2300	arg1	receptors					2302:2310	host innate immune receptors	2283:2310	host innate immune receptors	2283:2310	These findings indicate that the secretion of OMVs by B. fragilis may facilitate immune crosstalk with host epithelial cells at the gastrointestinal surface and suggests that OMVs produced by commensal bacteria may preferentially activate host innate immune receptors at the mucosal gastrointestinal tract.					
36304461	0	77	theme	membrane	27:34	arg1	vesicles					36:43	Bacteroides fragilis outer membrane vesicles	0:43	Bacteroides fragilis outer membrane vesicles	0:43	Bacteroides fragilis outer membrane vesicles preferentially activate innate immune receptors compared to their parent bacteria.					
36304461	1	78	theme	innate	278:283	arg1	responses					292:300	innate immune responses	278:300	innate immune responses	278:300	The release of bacterial membrane vesicles (BMVs) has become recognized as a key mechanism used by both pathogenic and commensal bacteria to activate innate immune responses in the host and mediate immunity.					
36304461	8	79	theme	broader	1841:1847	arg1	range					1849:1853	a broader range	1839:1853	a broader range of host innate immune receptors	1839:1885	Overall, our data demonstrates that B. fragilis OMVs activate a broader range of host innate immune receptors compared to their parent bacteria due to their enrichment of biological cargo and their ability to transport this cargo directly into host epithelial cells.					
36304461	2	80	theme	immunogenic	421:431	arg1	cargo					433:437	various immunogenic cargo	413:437	various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan	413:493	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria can harbor various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan, and the composition of OMVs strongly influences their ability to activate host innate immune receptors.					
36304461	9	81	theme	mucosal	2319:2325	arg1	tract					2344:2348	the mucosal gastrointestinal tract	2315:2348	the mucosal gastrointestinal tract	2315:2348	These findings indicate that the secretion of OMVs by B. fragilis may facilitate immune crosstalk with host epithelial cells at the gastrointestinal surface and suggests that OMVs produced by commensal bacteria may preferentially activate host innate immune receptors at the mucosal gastrointestinal tract.					
36304461	7	82	theme	oligomerization	1654:1668	arg1	protein					1688:1694	nucleotide-binding oligomerization domain-containing protein 1	1635:1696	nucleotide-binding oligomerization domain-containing protein 1	1635:1696	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	82	theme	oligomerization	1654:1668	arg1	receptors					1601:1609	host Toll-like receptors	1586:1609	host Toll-like receptors (TLR)	1586:1615	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	2	83	theme	nucleic	463:469	arg1	acids					471:475	nucleic acids	463:475	nucleic acids	463:475	Outer membrane vesicles (OMVs) produced by Gram-negative bacteria can harbor various immunogenic cargo that includes proteins, nucleic acids and peptidoglycan, and the composition of OMVs strongly influences their ability to activate host innate immune receptors.					
36304461	0	84	theme	innate	69:74	arg1	receptors					83:91	innate immune receptors	69:91	innate immune receptors	69:91	Bacteroides fragilis outer membrane vesicles preferentially activate innate immune receptors compared to their parent bacteria.					
36304461	4	85	theme	innate	1030:1035	arg1	receptors					1044:1052	host innate immune receptors	1025:1052	host innate immune receptors	1025:1052	In this study, we investigated the cargo associated with OMVs produced by the intestinal commensal Bacteroides fragilis and determined their ability to activate host innate immune receptors.					
36304461	3	86	theme	immunogenic	679:689	arg1	cargo					691:695	immunogenic cargo	679:695	immunogenic cargo	679:695	Although various Gram-negative pathogens can produce OMVs that are enriched in immunogenic cargo compared to their parent bacteria, the ability of OMVs produced by commensal organisms to be enriched with immunostimulatory contents is only recently becoming known.					
36304461	7	87	dep	receptors	1601:1609	arg1	-2					1616:1617	-2	1616:1617	-2	1616:1617	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	7	88	theme	reporter	1514:1521	arg1	lines					1528:1532	HEK-Blue reporter cell lines	1505:1532	HEK-Blue reporter cell lines	1505:1532	Using HEK-Blue reporter cell lines, we identified that B. fragilis OMVs could activate host Toll-like receptors (TLR)-2, TLR4, TLR7 and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), whereas B. fragilis bacteria could only induce the activation of TLR2.					
36304461	6	89	theme	Caco-2	1474:1479	arg1	cells					1492:1496	Caco-2 epithelial cells	1474:1496	Caco-2 epithelial cells	1474:1496	We visualized the entry of B. fragilis OMVs into intestinal epithelial cells, in addition to the ability of B. fragilis OMVs to transport bacterial RNA and peptidoglycan cargo into Caco-2 epithelial cells.					
36304461	1	90	theme	pathogenic	232:241	arg1	bacteria					257:264	both pathogenic and commensal bacteria	227:264	both pathogenic and commensal bacteria	227:264	The release of bacterial membrane vesicles (BMVs) has become recognized as a key mechanism used by both pathogenic and commensal bacteria to activate innate immune responses in the host and mediate immunity.					
36623628	5	0	theme	antibacterial	825:837	arg1	activity					839:846	desirable antibacterial activity	815:846	desirable antibacterial activity	815:846	In vitro, this hydrogel exhibited self-healing ability and excellent biocompatibility, promoted macrophage polarization towards M2 phenotype, and showed desirable antibacterial activity.					
36623628	7	1	theme	novel	1273:1277	arg1	target					1279:1284	a novel target	1271:1284	a novel target for developing efficient wound healing tools	1271:1329	This work revealed the potential immunomodulatory effect of CS-based wound dressings and thus may provide a novel target for developing efficient wound healing tools.					
36623628	7	2	theme	efficient	1301:1309	arg1	healing					1317:1323	efficient wound healing	1301:1323	efficient wound healing tools	1301:1329	This work revealed the potential immunomodulatory effect of CS-based wound dressings and thus may provide a novel target for developing efficient wound healing tools.					
36623628	4	3	theme	ammonium	625:632	arg1	hydrogel					652:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	Herein, we developed a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel.					
36623628	0	4	theme	immunomodulatory	80:95	arg1	effects					97:103	immunomodulatory effects	80:103	immunomodulatory effects	80:103	A multifunctional chitosan-based hydrogel with self-healing, antibacterial, and immunomodulatory effects as wound dressing.					
36623628	2	5	theme	wound	366:370	arg1	dressings					372:380	wound dressings	366:380	wound dressings	366:380	Chitosan (CS)-based composite hydrogels can hold desirable mechanical properties and maintain excellent antibacterial abilities, and thus may be promising as wound dressings.					
36623628	4	6	theme	trimethyl	615:623	arg1	hydrogel					652:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	Herein, we developed a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel.					
36623628	0	7	theme	wound	108:112	arg1	dressing					114:121	wound dressing	108:121	wound dressing	108:121	A multifunctional chitosan-based hydrogel with self-healing, antibacterial, and immunomodulatory effects as wound dressing.					
36623628	5	8	theme	self-healing	696:707	arg1	ability					709:715	self-healing ability	696:715	self-healing ability	696:715	In vitro, this hydrogel exhibited self-healing ability and excellent biocompatibility, promoted macrophage polarization towards M2 phenotype, and showed desirable antibacterial activity.					
36623628	2	9	theme	antibacterial	312:324	arg1	abilities					326:334	excellent antibacterial abilities	302:334	excellent antibacterial abilities	302:334	Chitosan (CS)-based composite hydrogels can hold desirable mechanical properties and maintain excellent antibacterial abilities, and thus may be promising as wound dressings.					
36623628	7	10	theme	wound	1311:1315	arg1	healing					1317:1323	efficient wound healing	1301:1323	efficient wound healing tools	1301:1329	This work revealed the potential immunomodulatory effect of CS-based wound dressings and thus may provide a novel target for developing efficient wound healing tools.					
36623628	2	11	theme	excellent	302:310	arg1	abilities					326:334	excellent antibacterial abilities	302:334	excellent antibacterial abilities	302:334	Chitosan (CS)-based composite hydrogels can hold desirable mechanical properties and maintain excellent antibacterial abilities, and thus may be promising as wound dressings.					
36623628	4	12	theme	chloride	634:641	arg1	hydrogel					652:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	Herein, we developed a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel.					
36623628	6	13	theme	regulatory	1138:1147	arg1	cells					1158:1162	anti-inflammatory regulatory T (Treg) cells	1120:1162	anti-inflammatory regulatory T (Treg) cells	1120:1162	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	3	14	theme	wound-healing	461:473	arg1	abilities					475:483	the antibacterial and wound-healing abilities	439:483	the antibacterial and wound-healing abilities	439:483	Although CS-based hydrogels have been widely studied on the antibacterial and wound-healing abilities, their immunomodulatory abilities were rarely evaluated.					
36623628	6	15	theme	collagen	956:963	arg1	deposition					965:974	collagen deposition	956:974	collagen deposition	956:974	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	6	16	theme	T	1082:1082	arg1	cells					1106:1110	T helper type 17 (Th17) cells	1082:1110	T helper type 17 (Th17) cells	1082:1110	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	1	17	theme	wound	193:197	arg1	healing					199:205	wound healing	193:205	wound healing	193:205	Bacterial infection often leads to inflammatory responses and delays wound healing.					
36623628	0	18	theme	multifunctional	2:16	arg1	hydrogel					33:40	A multifunctional chitosan-based hydrogel	0:40	A multifunctional chitosan-based hydrogel with self-healing, antibacterial, and immunomodulatory effects as wound dressing.	0:122	A multifunctional chitosan-based hydrogel with self-healing, antibacterial, and immunomodulatory effects as wound dressing.					
36623628	7	19	theme	dressings	1240:1248	arg1	effect					1215:1220	the potential immunomodulatory effect	1184:1220	the potential immunomodulatory effect of CS-based wound dressings	1184:1248	This work revealed the potential immunomodulatory effect of CS-based wound dressings and thus may provide a novel target for developing efficient wound healing tools.					
36623628	3	20	theme	antibacterial	443:455	arg1	abilities					475:483	the antibacterial and wound-healing abilities	439:483	the antibacterial and wound-healing abilities	439:483	Although CS-based hydrogels have been widely studied on the antibacterial and wound-healing abilities, their immunomodulatory abilities were rarely evaluated.					
36623628	6	21	theme	macrophage	1013:1022	arg1	polarization					1024:1035	macrophage polarization	1013:1035	macrophage polarization to M2 direction	1013:1051	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	6	22	theme	anti-inflammatory	1120:1136	arg1	cells					1158:1162	anti-inflammatory regulatory T (Treg) cells	1120:1162	anti-inflammatory regulatory T (Treg) cells	1120:1162	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	3	23	theme	CS-based	392:399	arg1	hydrogels					401:409	CS-based hydrogels	392:409	CS-based hydrogels	392:409	Although CS-based hydrogels have been widely studied on the antibacterial and wound-healing abilities, their immunomodulatory abilities were rarely evaluated.					
36623628	4	24	theme	methacryloyloxy	592:606	arg1	hydrogel					652:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	Herein, we developed a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel.					
36623628	3	25	theme	immunomodulatory	492:507	arg1	abilities					509:517	their immunomodulatory abilities	486:517	their immunomodulatory abilities	486:517	Although CS-based hydrogels have been widely studied on the antibacterial and wound-healing abilities, their immunomodulatory abilities were rarely evaluated.					
36623628	0	26	theme	chitosan-based	18:31	arg1	hydrogel					33:40	A multifunctional chitosan-based hydrogel	0:40	A multifunctional chitosan-based hydrogel with self-healing, antibacterial, and immunomodulatory effects as wound dressing.	0:122	A multifunctional chitosan-based hydrogel with self-healing, antibacterial, and immunomodulatory effects as wound dressing.					
36623628	5	27	theme	macrophage	758:767	arg1	polarization					769:780	macrophage polarization	758:780	macrophage polarization towards M2 phenotype	758:801	In vitro, this hydrogel exhibited self-healing ability and excellent biocompatibility, promoted macrophage polarization towards M2 phenotype, and showed desirable antibacterial activity.					
36623628	6	28	theme	Treg	1152:1155	arg1	cells					1158:1162	anti-inflammatory regulatory T (Treg) cells	1120:1162	anti-inflammatory regulatory T (Treg) cells	1120:1162	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	6	29	theme	cells	1106:1110	arg1	balance					1071:1077	the balance	1067:1077	the balance of T helper type 17 (Th17) cells	1067:1110	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	4	30	theme	ethyl	608:612	arg1	hydrogel					652:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	Herein, we developed a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel.					
36623628	4	31	theme	multifunctional	565:579	arg1	hydrogel					652:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	Herein, we developed a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel.					
36623628	6	32	theme	bacterial	927:935	arg1	burden					937:942	bacterial burden	927:942	bacterial burden	927:942	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	7	33	theme	CS-based	1225:1232	arg1	dressings					1240:1248	CS-based wound dressings	1225:1248	CS-based wound dressings	1225:1248	This work revealed the potential immunomodulatory effect of CS-based wound dressings and thus may provide a novel target for developing efficient wound healing tools.					
36623628	0	34	with	hydrogel	33:40	arg1	self-healing					47:58	self-healing	47:58	self-healing	47:58	A multifunctional chitosan-based hydrogel with self-healing, antibacterial, and immunomodulatory effects as wound dressing.					
36623628	0	34	with	hydrogel	33:40	arg1	effects					97:103	immunomodulatory effects	80:103	immunomodulatory effects	80:103	A multifunctional chitosan-based hydrogel with self-healing, antibacterial, and immunomodulatory effects as wound dressing.					
36623628	0	34	with	hydrogel	33:40	arg1	antibacterial					61:73	antibacterial	61:73	antibacterial	61:73	A multifunctional chitosan-based hydrogel with self-healing, antibacterial, and immunomodulatory effects as wound dressing.					
36623628	6	35	theme	Th17	1100:1103	arg1	cells					1106:1110	T helper type 17 (Th17) cells	1082:1110	T helper type 17 (Th17) cells	1082:1110	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	7	36	theme	potential	1188:1196	arg1	effect					1215:1220	the potential immunomodulatory effect	1184:1220	the potential immunomodulatory effect of CS-based wound dressings	1184:1248	This work revealed the potential immunomodulatory effect of CS-based wound dressings and thus may provide a novel target for developing efficient wound healing tools.					
36623628	5	37	theme	M2	790:791	arg1	phenotype					793:801	M2 phenotype	790:801	M2 phenotype	790:801	In vitro, this hydrogel exhibited self-healing ability and excellent biocompatibility, promoted macrophage polarization towards M2 phenotype, and showed desirable antibacterial activity.					
36623628	2	38	theme	composite	228:236	arg1	hydrogels					238:246	Chitosan (CS)-based composite hydrogels	208:246	Chitosan (CS)-based composite hydrogels	208:246	Chitosan (CS)-based composite hydrogels can hold desirable mechanical properties and maintain excellent antibacterial abilities, and thus may be promising as wound dressings.					
36623628	1	39	theme	Bacterial	124:132	arg1	infection					134:142	Bacterial infection	124:142	Bacterial infection	124:142	Bacterial infection often leads to inflammatory responses and delays wound healing.					
36623628	7	40	theme	immunomodulatory	1198:1213	arg1	effect					1215:1220	the potential immunomodulatory effect	1184:1220	the potential immunomodulatory effect of CS-based wound dressings	1184:1248	This work revealed the potential immunomodulatory effect of CS-based wound dressings and thus may provide a novel target for developing efficient wound healing tools.					
36623628	5	41	theme	excellent	721:729	arg1	biocompatibility					731:746	excellent biocompatibility	721:746	excellent biocompatibility	721:746	In vitro, this hydrogel exhibited self-healing ability and excellent biocompatibility, promoted macrophage polarization towards M2 phenotype, and showed desirable antibacterial activity.					
36623628	2	42	theme	-based	221:226	arg1	hydrogels					238:246	Chitosan (CS)-based composite hydrogels	208:246	Chitosan (CS)-based composite hydrogels	208:246	Chitosan (CS)-based composite hydrogels can hold desirable mechanical properties and maintain excellent antibacterial abilities, and thus may be promising as wound dressings.					
36623628	4	43	theme	CS/Poly[2-	581:590	arg1	hydrogel					652:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	Herein, we developed a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel.					
36623628	6	44	theme	M2	1040:1041	arg1	direction					1043:1051	M2 direction	1040:1051	M2 direction	1040:1051	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	6	45	theme	regeneration	894:905	arg1	process					907:913	the wound regeneration process	884:913	the wound regeneration process	884:913	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	2	46	theme	mechanical	267:276	arg1	properties					278:287	desirable mechanical properties	257:287	desirable mechanical properties	257:287	Chitosan (CS)-based composite hydrogels can hold desirable mechanical properties and maintain excellent antibacterial abilities, and thus may be promising as wound dressings.					
36623628	4	47	theme	PMETAC	644:649	arg1	hydrogel					652:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel	563:659	Herein, we developed a multifunctional CS/Poly[2-(methacryloyloxy)ethyl] trimethyl ammonium chloride (PMETAC) hydrogel.					
36623628	6	48	theme	wound	888:892	arg1	process					907:913	the wound regeneration process	884:913	the wound regeneration process	884:913	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	2	49	theme	desirable	257:265	arg1	properties					278:287	desirable mechanical properties	257:287	desirable mechanical properties	257:287	Chitosan (CS)-based composite hydrogels can hold desirable mechanical properties and maintain excellent antibacterial abilities, and thus may be promising as wound dressings.					
36623628	7	50	theme	healing	1317:1323	arg1	tools					1325:1329	efficient wound healing tools	1301:1329	efficient wound healing tools	1301:1329	This work revealed the potential immunomodulatory effect of CS-based wound dressings and thus may provide a novel target for developing efficient wound healing tools.					
36623628	6	51	theme	T	1149:1149	arg1	cells					1158:1162	anti-inflammatory regulatory T (Treg) cells	1120:1162	anti-inflammatory regulatory T (Treg) cells	1120:1162	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	6	52	theme	type	1091:1094	arg1	cells					1106:1110	T helper type 17 (Th17) cells	1082:1110	T helper type 17 (Th17) cells	1082:1110	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
36623628	5	53	theme	desirable	815:823	arg1	activity					839:846	desirable antibacterial activity	815:846	desirable antibacterial activity	815:846	In vitro, this hydrogel exhibited self-healing ability and excellent biocompatibility, promoted macrophage polarization towards M2 phenotype, and showed desirable antibacterial activity.					
36623628	7	54	theme	wound	1234:1238	arg1	dressings					1240:1248	CS-based wound dressings	1225:1248	CS-based wound dressings	1225:1248	This work revealed the potential immunomodulatory effect of CS-based wound dressings and thus may provide a novel target for developing efficient wound healing tools.					
36623628	1	55	theme	inflammatory	159:170	arg1	responses					172:180	inflammatory responses	159:180	inflammatory responses	159:180	Bacterial infection often leads to inflammatory responses and delays wound healing.					
36623628	6	56	theme	helper	1084:1089	arg1	cells					1106:1110	T helper type 17 (Th17) cells	1082:1110	T helper type 17 (Th17) cells	1082:1110	In vivo, this hydrogel accelerated the wound regeneration process by reducing bacterial burden, increasing collagen deposition, stimulating angiogenesis, promoting macrophage polarization to M2 direction, and shifting the balance of T helper type 17 (Th17) cells towards anti-inflammatory regulatory T (Treg) cells.					
35385812	7	0	theme	antioxidant	1628:1638	arg1	properties					1659:1668	the mechanical, antioxidant, and antibacterial properties	1612:1668	the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films	1612:1692	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	2	1	theme	GA-UT-TSC-CTS	511:523	arg1	film					525:528	the GA-UT-TSC-CTS film	507:528	the GA-UT-TSC-CTS film	507:528	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	6	2	theme	antibacterial	1339:1351	arg1	effect					1353:1358	The antibacterial effect	1335:1358	The antibacterial effect of the film against Bacillus subtilis	1335:1396	The antibacterial effect of the film against Bacillus subtilis was the highest, followed by Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus.					
35385812	6	2	theme	antibacterial	1339:1351	arg1	highest					1406:1412	highest	1406:1412	highest	1406:1412	The antibacterial effect of the film against Bacillus subtilis was the highest, followed by Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus.					
35385812	0	3	theme	antioxidant	80:90	arg1	properties					111:120	mechanical, antioxidant, and antibacterial properties	68:120	mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film	68:156	The modification of pomegranate polyphenol with ultrasound improves mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film.					
35385812	1	4	theme	pomegranate	343:353	arg1	acid					425:428	ellagic acid	417:428	ellagic acid (EA)	417:433	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	1	4	theme	pomegranate	343:353	arg1	polyphenols					355:365	pomegranate polyphenols	343:365	pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA)	343:433	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	1	4	theme	pomegranate	343:353	arg1	acid					384:387	gallic acid	377:387	gallic acid (GA)	377:392	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	1	4	theme	pomegranate	343:353	arg1	acid					402:405	tannic acid	395:405	tannic acid (TA)	395:410	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	2	5	theme	28 ± 0.5 kHz	555:566	arg1	cycle					603:607	sweep frequency cycle	587:607	sweep frequency cycle of 100 ms	587:617	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	5	theme	28 ± 0.5 kHz	555:566	arg1	time					699:702	reaction time	690:702	reaction time	690:702	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	5	theme	28 ± 0.5 kHz	555:566	arg1	power					569:573	power	569:573	power of 100 W/L	569:584	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	5	theme	28 ± 0.5 kHz	555:566	arg1	frequency					542:550	ultrasound frequency	531:550	ultrasound frequency of 28 ± 0.5 kHz	531:566	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	5	theme	28 ± 0.5 kHz	555:566	arg1	time					642:645	time	642:645	time of 10 min; GA concentration of 1.0 g/L	642:684	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	5	theme	28 ± 0.5 kHz	555:566	arg1	ratio					625:629	duty ratio	620:629	duty ratio of 77%	620:636	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	7	6	theme	films	1688:1692	arg1	properties					1659:1668	the mechanical, antioxidant, and antibacterial properties	1612:1668	the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films	1612:1692	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	4	7	theme	X-ray	1155:1159	arg1	XRD					1174:1176	XRD	1174:1176	XRD	1174:1176	These positive modification results were attributed to the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results.					
35385812	4	7	theme	X-ray	1155:1159	arg1	diffraction					1161:1171	X-ray diffraction	1155:1171	X-ray diffraction (XRD)	1155:1177	These positive modification results were attributed to the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results.					
35385812	7	8	theme	edible	1673:1678	arg1	films					1688:1692	edible TSC-CTS films	1673:1692	edible TSC-CTS films	1673:1692	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	1	9	theme	TSC-CTS	267:273	arg1	films					286:290	Tuna skin collagen-chitosan (TSC-CTS) composite films	238:290	Tuna skin collagen-chitosan (TSC-CTS) composite films	238:290	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	0	10	theme	antibacterial	97:109	arg1	properties					111:120	mechanical, antioxidant, and antibacterial properties	68:120	mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film	68:156	The modification of pomegranate polyphenol with ultrasound improves mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film.					
35385812	4	11	theme	film	1035:1038	arg1	process					1050:1056	the film formation process	1031:1056	the film formation process	1031:1056	These positive modification results were attributed to the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results.					
35385812	2	12	theme	frequency	593:601	arg1	cycle					603:607	sweep frequency cycle	587:607	sweep frequency cycle of 100 ms	587:617	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	5	13	theme	GA-UT-TSC-CTS	1224:1236	arg1	film					1238:1241	the GA-UT-TSC-CTS film	1220:1241	the GA-UT-TSC-CTS film	1220:1241	Moreover, the GA-UT-TSC-CTS film possessed moderate thermal stability and color indexes and improved antibacterial activity.					
35385812	6	14	theme	Listeria	1445:1452	arg1	monocytogenes					1454:1466	Listeria monocytogenes	1445:1466	Listeria monocytogenes	1445:1466	The antibacterial effect of the film against Bacillus subtilis was the highest, followed by Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus.					
35385812	5	15	theme	moderate	1253:1260	arg1	stability					1270:1278	moderate thermal stability	1253:1278	moderate thermal stability	1253:1278	Moreover, the GA-UT-TSC-CTS film possessed moderate thermal stability and color indexes and improved antibacterial activity.					
35385812	3	16	theme	vapor	851:855	arg1	permeability					857:868	water vapor permeability	845:868	water vapor permeability	845:868	Meanwhile, light transmittance and water vapor permeability of the GA-UT-TSC-CTS film were decreased by 29.26% and 15.70%, respectively.					
35385812	1	17	theme	ellagic	417:423	arg1	EA					431:432	EA	431:432	EA	431:432	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	1	17	theme	ellagic	417:423	arg1	acid					425:428	ellagic acid	417:428	ellagic acid (EA)	417:433	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	7	18	theme	mechanical	1616:1625	arg1	properties					1659:1668	the mechanical, antioxidant, and antibacterial properties	1612:1668	the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films	1612:1692	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	2	19	theme	1.0 g/L	678:684	arg1	cycle					603:607	sweep frequency cycle	587:607	sweep frequency cycle of 100 ms	587:617	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	19	theme	1.0 g/L	678:684	arg1	time					699:702	reaction time	690:702	reaction time	690:702	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	19	theme	1.0 g/L	678:684	arg1	power					569:573	power	569:573	power of 100 W/L	569:584	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	19	theme	1.0 g/L	678:684	arg1	frequency					542:550	ultrasound frequency	531:550	ultrasound frequency of 28 ± 0.5 kHz	531:566	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	19	theme	1.0 g/L	678:684	arg1	time					642:645	time	642:645	time of 10 min; GA concentration of 1.0 g/L	642:684	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	19	theme	1.0 g/L	678:684	arg1	ratio					625:629	duty ratio	620:629	duty ratio of 77%	620:636	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	1	20	theme	composite	276:284	arg1	films					286:290	Tuna skin collagen-chitosan (TSC-CTS) composite films	238:290	Tuna skin collagen-chitosan (TSC-CTS) composite films	238:290	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	1	21	theme	gallic	377:382	arg1	GA					390:391	GA	390:391	GA	390:391	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	1	21	theme	gallic	377:382	arg1	acid					384:387	gallic acid	377:387	gallic acid (GA)	377:392	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	2	22	theme	sweep	587:591	arg1	cycle					603:607	sweep frequency cycle	587:607	sweep frequency cycle of 100 ms	587:617	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	4	23	theme	altered	1006:1012	arg1	structure					1014:1022	the altered structure	1002:1022	the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results	1002:1207	These positive modification results were attributed to the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results.					
35385812	1	24	theme	edible	173:178	arg1	film					180:183	an edible film	170:183	an edible film with high mechanical and physicochemical properties	170:235	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	7	25	mod	modification	1521:1532	arg1	ultrasound					1553:1562	ultrasound	1553:1562	ultrasound	1553:1562	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	7	25	mod	modification	1521:1532	arg1	acid					1544:1547	gallic acid	1537:1547	gallic acid	1537:1547	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	7	25	mod	modification	1521:1532	arg3	combination					1509:1519	the combination modification	1505:1532	the combination modification of gallic acid and ultrasound	1505:1562	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	2	26	theme	100 W/L	578:584	arg1	cycle					603:607	sweep frequency cycle	587:607	sweep frequency cycle of 100 ms	587:617	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	26	theme	100 W/L	578:584	arg1	time					699:702	reaction time	690:702	reaction time	690:702	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	26	theme	100 W/L	578:584	arg1	power					569:573	power	569:573	power of 100 W/L	569:584	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	26	theme	100 W/L	578:584	arg1	frequency					542:550	ultrasound frequency	531:550	ultrasound frequency of 28 ± 0.5 kHz	531:566	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	26	theme	100 W/L	578:584	arg1	time					642:645	time	642:645	time of 10 min; GA concentration of 1.0 g/L	642:684	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	26	theme	100 W/L	578:584	arg1	ratio					625:629	duty ratio	620:629	duty ratio of 77%	620:636	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	27	theme	concentration	661:673	arg1	cycle					603:607	sweep frequency cycle	587:607	sweep frequency cycle of 100 ms	587:617	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	27	theme	concentration	661:673	arg1	time					699:702	reaction time	690:702	reaction time	690:702	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	27	theme	concentration	661:673	arg1	power					569:573	power	569:573	power of 100 W/L	569:584	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	27	theme	concentration	661:673	arg1	frequency					542:550	ultrasound frequency	531:550	ultrasound frequency of 28 ± 0.5 kHz	531:566	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	27	theme	concentration	661:673	arg1	time					642:645	time	642:645	time of 10 min; GA concentration of 1.0 g/L	642:684	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	27	theme	concentration	661:673	arg1	ratio					625:629	duty ratio	620:629	duty ratio of 77%	620:636	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	6	28	theme	film	1367:1370	arg1	effect					1353:1358	The antibacterial effect	1335:1358	The antibacterial effect of the film against Bacillus subtilis	1335:1396	The antibacterial effect of the film against Bacillus subtilis was the highest, followed by Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus.					
35385812	6	28	theme	film	1367:1370	arg1	highest					1406:1412	highest	1406:1412	highest	1406:1412	The antibacterial effect of the film against Bacillus subtilis was the highest, followed by Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus.					
35385812	2	29	theme	duty	620:623	arg1	ratio					625:629	duty ratio	620:629	duty ratio of 77%	620:636	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	30	theme	GA	658:659	arg1	concentration					661:673	10 min; GA concentration	650:673	10 min; GA concentration	650:673	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	4	31	dep	Fourier	1082:1088	arg1	transform					1090:1098	transform	1090:1098	transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results	1090:1207	These positive modification results were attributed to the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results.					
35385812	5	32	theme	antibacterial	1311:1323	arg1	activity					1325:1332	antibacterial activity	1311:1332	antibacterial activity	1311:1332	Moreover, the GA-UT-TSC-CTS film possessed moderate thermal stability and color indexes and improved antibacterial activity.					
35385812	1	33	theme	high	190:193	arg1	properties					226:235	high mechanical and physicochemical properties	190:235	high mechanical and physicochemical properties	190:235	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	0	34	theme	pomegranate	20:30	arg1	polyphenol					32:41	pomegranate polyphenol	20:41	pomegranate polyphenol	20:41	The modification of pomegranate polyphenol with ultrasound improves mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film.					
35385812	5	35	theme	color	1284:1288	arg1	indexes					1290:1296	color indexes	1284:1296	color indexes	1284:1296	Moreover, the GA-UT-TSC-CTS film possessed moderate thermal stability and color indexes and improved antibacterial activity.					
35385812	0	36	theme	skin	130:133	arg1	film					153:156	tuna skin collagen-chitosan film	125:156	tuna skin collagen-chitosan film	125:156	The modification of pomegranate polyphenol with ultrasound improves mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film.					
35385812	2	37	theme	100 ms	612:617	arg1	cycle					603:607	sweep frequency cycle	587:607	sweep frequency cycle of 100 ms	587:617	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	37	theme	100 ms	612:617	arg1	time					699:702	reaction time	690:702	reaction time	690:702	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	37	theme	100 ms	612:617	arg1	power					569:573	power	569:573	power of 100 W/L	569:584	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	37	theme	100 ms	612:617	arg1	frequency					542:550	ultrasound frequency	531:550	ultrasound frequency of 28 ± 0.5 kHz	531:566	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	37	theme	100 ms	612:617	arg1	time					642:645	time	642:645	time of 10 min; GA concentration of 1.0 g/L	642:684	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	37	theme	100 ms	612:617	arg1	ratio					625:629	duty ratio	620:629	duty ratio of 77%	620:636	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	3	38	theme	light	821:825	arg1	transmittance					827:839	light transmittance	821:839	light transmittance	821:839	Meanwhile, light transmittance and water vapor permeability of the GA-UT-TSC-CTS film were decreased by 29.26% and 15.70%, respectively.					
35385812	1	39	theme	mechanical	195:204	arg1	properties					226:235	high mechanical and physicochemical properties	190:235	high mechanical and physicochemical properties	190:235	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	3	40	theme	GA-UT-TSC-CTS	877:889	arg1	film					891:894	the GA-UT-TSC-CTS film	873:894	the GA-UT-TSC-CTS film	873:894	Meanwhile, light transmittance and water vapor permeability of the GA-UT-TSC-CTS film were decreased by 29.26% and 15.70%, respectively.					
35385812	2	41	theme	TSC-CTS	795:801	arg1	film					803:806	TSC-CTS film	795:806	TSC-CTS film	795:806	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	41	theme	TSC-CTS	795:801	arg1	control					786:792	the control	782:792	the control (TSC-CTS film)	782:807	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	0	42	theme	tuna	125:128	arg1	film					153:156	tuna skin collagen-chitosan film	125:156	tuna skin collagen-chitosan film	125:156	The modification of pomegranate polyphenol with ultrasound improves mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film.					
35385812	7	43	theme	acid	1544:1547	arg1	method					1594:1599	an efficient modification method	1568:1599	an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films	1568:1692	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	7	43	theme	acid	1544:1547	arg1	modification					1521:1532	the combination modification	1505:1532	the combination modification of gallic acid and ultrasound	1505:1562	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	2	44	theme	reaction	690:697	arg1	time					699:702	reaction time	690:702	reaction time	690:702	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	7	45	theme	modification	1581:1592	arg1	method					1594:1599	an efficient modification method	1568:1599	an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films	1568:1692	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	7	45	theme	modification	1581:1592	arg1	modification					1521:1532	the combination modification	1505:1532	the combination modification of gallic acid and ultrasound	1505:1562	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	0	46	theme	film	153:156	arg1	properties					111:120	mechanical, antioxidant, and antibacterial properties	68:120	mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film	68:156	The modification of pomegranate polyphenol with ultrasound improves mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film.					
35385812	7	47	theme	combination	1509:1519	arg1	method					1594:1599	an efficient modification method	1568:1599	an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films	1568:1692	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	7	47	theme	combination	1509:1519	arg1	modification					1521:1532	the combination modification	1505:1532	the combination modification of gallic acid and ultrasound	1505:1562	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	4	48	theme	modification	962:973	arg1	results					975:981	These positive modification results	947:981	These positive modification results	947:981	These positive modification results were attributed to the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results.					
35385812	1	49	theme	physicochemical	210:224	arg1	properties					226:235	high mechanical and physicochemical properties	190:235	high mechanical and physicochemical properties	190:235	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	0	50	theme	polyphenol	32:41	arg1	modification					4:15	The modification	0:15	The modification of pomegranate polyphenol with ultrasound	0:57	The modification of pomegranate polyphenol with ultrasound improves mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film.					
35385812	0	51	theme	collagen-chitosan	135:151	arg1	film					153:156	tuna skin collagen-chitosan film	125:156	tuna skin collagen-chitosan film	125:156	The modification of pomegranate polyphenol with ultrasound improves mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film.					
35385812	7	52	theme	TSC-CTS	1680:1686	arg1	films					1688:1692	edible TSC-CTS films	1673:1692	edible TSC-CTS films	1673:1692	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	3	53	theme	water	845:849	arg1	permeability					857:868	water vapor permeability	845:868	water vapor permeability	845:868	Meanwhile, light transmittance and water vapor permeability of the GA-UT-TSC-CTS film were decreased by 29.26% and 15.70%, respectively.					
35385812	7	54	theme	efficient	1571:1579	arg1	method					1594:1599	an efficient modification method	1568:1599	an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films	1568:1692	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	7	54	theme	efficient	1571:1579	arg1	modification					1521:1532	the combination modification	1505:1532	the combination modification of gallic acid and ultrasound	1505:1562	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	2	55	theme	DPPH	479:482	arg1	activity					495:502	the DPPH scavenging activity	475:502	the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min)	475:713	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	56	theme	ultrasound	531:540	arg1	frequency					542:550	ultrasound frequency	531:550	ultrasound frequency of 28 ± 0.5 kHz	531:566	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	1	57	theme	tannic	395:400	arg1	TA					408:409	TA	408:409	TA	408:409	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	1	57	theme	tannic	395:400	arg1	acid					402:405	tannic acid	395:405	tannic acid (TA)	395:410	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	2	58	theme	%	636:636	arg1	cycle					603:607	sweep frequency cycle	587:607	sweep frequency cycle of 100 ms	587:617	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	58	theme	%	636:636	arg1	time					699:702	reaction time	690:702	reaction time	690:702	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	58	theme	%	636:636	arg1	power					569:573	power	569:573	power of 100 W/L	569:584	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	58	theme	%	636:636	arg1	frequency					542:550	ultrasound frequency	531:550	ultrasound frequency of 28 ± 0.5 kHz	531:566	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	58	theme	%	636:636	arg1	time					642:645	time	642:645	time of 10 min; GA concentration of 1.0 g/L	642:684	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	58	theme	%	636:636	arg1	ratio					625:629	duty ratio	620:629	duty ratio of 77%	620:636	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	59	dep	activity	495:502	arg1	cycle					603:607	sweep frequency cycle	587:607	sweep frequency cycle of 100 ms	587:617	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	59	dep	activity	495:502	arg1	frequency					542:550	ultrasound frequency	531:550	ultrasound frequency of 28 ± 0.5 kHz	531:566	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	59	dep	activity	495:502	arg1	time					642:645	time	642:645	time of 10 min; GA concentration of 1.0 g/L	642:684	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	59	dep	activity	495:502	arg1	power					569:573	power	569:573	power of 100 W/L	569:584	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	59	dep	activity	495:502	arg1	ratio					625:629	duty ratio	620:629	duty ratio of 77%	620:636	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	60	theme	scavenging	484:493	arg1	activity					495:502	the DPPH scavenging activity	475:502	the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min)	475:713	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	4	61	theme	circular	1130:1137	arg1	CD					1150:1151	CD	1150:1151	CD	1150:1151	These positive modification results were attributed to the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results.					
35385812	4	61	theme	circular	1130:1137	arg1	dichroism					1139:1147	circular dichroism	1130:1147	circular dichroism (CD)	1130:1152	These positive modification results were attributed to the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results.					
35385812	0	62	with	modification	4:15	arg1	ultrasound					48:57	ultrasound	48:57	ultrasound	48:57	The modification of pomegranate polyphenol with ultrasound improves mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film.					
35385812	3	63	theme	film	891:894	arg1	Meanwhile					810:818	Meanwhile	810:818	Meanwhile	810:818	Meanwhile, light transmittance and water vapor permeability of the GA-UT-TSC-CTS film were decreased by 29.26% and 15.70%, respectively.					
35385812	3	63	theme	film	891:894	arg1	transmittance					827:839	light transmittance	821:839	light transmittance	821:839	Meanwhile, light transmittance and water vapor permeability of the GA-UT-TSC-CTS film were decreased by 29.26% and 15.70%, respectively.					
35385812	3	63	theme	film	891:894	arg1	permeability					857:868	water vapor permeability	845:868	water vapor permeability	845:868	Meanwhile, light transmittance and water vapor permeability of the GA-UT-TSC-CTS film were decreased by 29.26% and 15.70%, respectively.					
35385812	7	64	theme	antibacterial	1645:1657	arg1	properties					1659:1668	the mechanical, antioxidant, and antibacterial properties	1612:1668	the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films	1612:1692	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	2	65	theme	film	525:528	arg1	activity					495:502	the DPPH scavenging activity	475:502	the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min)	475:713	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	65	theme	film	525:528	arg1	strength					462:469	The tensile strength	450:469	The tensile strength	450:469	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	66	dep	24.16	744:748	arg1	folds					750:754	folds	750:754	folds	750:754	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	5	67	theme	thermal	1262:1268	arg1	stability					1270:1278	moderate thermal stability	1253:1278	moderate thermal stability	1253:1278	Moreover, the GA-UT-TSC-CTS film possessed moderate thermal stability and color indexes and improved antibacterial activity.					
35385812	5	68	contain	possessed	1243:1251	arg2	stability					1270:1278	moderate thermal stability	1253:1278	moderate thermal stability	1253:1278	Moreover, the GA-UT-TSC-CTS film possessed moderate thermal stability and color indexes and improved antibacterial activity.					
35385812	5	68	contain	possessed	1243:1251	arg2	indexes					1290:1296	color indexes	1284:1296	color indexes	1284:1296	Moreover, the GA-UT-TSC-CTS film possessed moderate thermal stability and color indexes and improved antibacterial activity.					
35385812	5	68	contain	possessed	1243:1251	arg1	film					1238:1241	the GA-UT-TSC-CTS film	1220:1241	the GA-UT-TSC-CTS film	1220:1241	Moreover, the GA-UT-TSC-CTS film possessed moderate thermal stability and color indexes and improved antibacterial activity.					
35385812	4	69	theme	positive	953:960	arg1	results					975:981	These positive modification results	947:981	These positive modification results	947:981	These positive modification results were attributed to the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results.					
35385812	1	70	with	film	180:183	arg1	properties					226:235	high mechanical and physicochemical properties	190:235	high mechanical and physicochemical properties	190:235	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	4	71	theme	formation	1040:1048	arg1	process					1050:1056	the film formation process	1031:1056	the film formation process	1031:1056	These positive modification results were attributed to the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results.					
35385812	7	72	theme	ultrasound	1553:1562	arg1	method					1594:1599	an efficient modification method	1568:1599	an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films	1568:1692	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	7	72	theme	ultrasound	1553:1562	arg1	modification					1521:1532	the combination modification	1505:1532	the combination modification of gallic acid and ultrasound	1505:1562	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	4	73	dep	transform	1090:1098	arg1	infrared					1100:1107	infrared	1100:1107	transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results	1090:1207	These positive modification results were attributed to the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results.					
35385812	1	74	theme	Tuna	238:241	arg1	films					286:290	Tuna skin collagen-chitosan (TSC-CTS) composite films	238:290	Tuna skin collagen-chitosan (TSC-CTS) composite films	238:290	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	2	75	theme	tensile	454:460	arg1	strength					462:469	The tensile strength	450:469	The tensile strength	450:469	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	7	76	theme	gallic	1537:1542	arg1	acid					1544:1547	gallic acid	1537:1547	gallic acid	1537:1547	Overall, the combination modification of gallic acid and ultrasound was an efficient modification method to improve the mechanical, antioxidant, and antibacterial properties of edible TSC-CTS films.					
35385812	2	77	theme	10 min	650:655	arg1	concentration					661:673	10 min; GA concentration	650:673	10 min; GA concentration	650:673	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	78	theme	10 min	707:712	arg1	cycle					603:607	sweep frequency cycle	587:607	sweep frequency cycle of 100 ms	587:617	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	78	theme	10 min	707:712	arg1	time					699:702	reaction time	690:702	reaction time	690:702	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	78	theme	10 min	707:712	arg1	power					569:573	power	569:573	power of 100 W/L	569:584	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	78	theme	10 min	707:712	arg1	frequency					542:550	ultrasound frequency	531:550	ultrasound frequency of 28 ± 0.5 kHz	531:566	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	78	theme	10 min	707:712	arg1	time					642:645	time	642:645	time of 10 min; GA concentration of 1.0 g/L	642:684	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	2	78	theme	10 min	707:712	arg1	ratio					625:629	duty ratio	620:629	duty ratio of 77%	620:636	The tensile strength and the DPPH scavenging activity of the GA-UT-TSC-CTS film (ultrasound frequency of 28 ± 0.5 kHz, power of 100 W/L, sweep frequency cycle of 100 ms, duty ratio of 77% and time of 10 min; GA concentration of 1.0 g/L and reaction time of 10 min) were increased by 47.03% and 24.16 folds, respectively compared to the control (TSC-CTS film).					
35385812	1	79	theme	skin	243:246	arg1	films					286:290	Tuna skin collagen-chitosan (TSC-CTS) composite films	238:290	Tuna skin collagen-chitosan (TSC-CTS) composite films	238:290	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
35385812	0	80	theme	mechanical	68:77	arg1	properties					111:120	mechanical, antioxidant, and antibacterial properties	68:120	mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film	68:156	The modification of pomegranate polyphenol with ultrasound improves mechanical, antioxidant, and antibacterial properties of tuna skin collagen-chitosan film.					
35385812	4	81	theme	thermogravimetry	1184:1199	arg1	results					1201:1207	thermogravimetry results	1184:1207	thermogravimetry results	1184:1207	These positive modification results were attributed to the altered structure during the film formation process, which were verified by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), X-ray diffraction (XRD), and thermogravimetry results.					
35385812	1	82	theme	collagen-chitosan	248:264	arg1	films					286:290	Tuna skin collagen-chitosan (TSC-CTS) composite films	238:290	Tuna skin collagen-chitosan (TSC-CTS) composite films	238:290	To produce an edible film with high mechanical and physicochemical properties, Tuna skin collagen-chitosan (TSC-CTS) composite films were prepared by incorporating ultrasound (UT) and pomegranate polyphenols including gallic acid (GA), tannic acid (TA), and ellagic acid (EA), respectively.					
36610508	8	0	theme	viable	1616:1621	arg1	option					1623:1628	the most viable option	1607:1628	the most viable option for long-term AnMBR operation	1607:1658	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	8	0	theme	viable	1616:1621	arg1	synergism					1562:1570	the synergism	1558:1570	the synergism of exogenous and immobilized QQ	1558:1602	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	3	1	theme	filtration	695:704	arg1	operation					706:714	the membrane filtration operation	682:714	the membrane filtration operation	682:714	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	2	2	theme	identical	362:370	arg1	AnMBRs					404:409	AnMBRs	404:409	AnMBRs	404:409	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	2	2	theme	identical	362:370	arg1	bioreactors					391:401	Two identical anaerobic membrane bioreactors	358:401	Two identical anaerobic membrane bioreactors (AnMBRs)	358:410	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	7	3	theme	biofouling	1439:1448	arg1	cause					1430:1434	the ultimate cause	1417:1434	the ultimate cause of biofouling	1417:1448	The QQ significantly altered the biofilm-forming community in mixed liquor, while the members with robust signal transduction systems became dominant to counteract the QQ mechanism and were the ultimate cause of biofouling.					
36610508	6	4	theme	signal	1118:1123	arg1	molecules					1125:1133	targeted signal molecules	1109:1133	targeted signal molecules	1109:1133	Therefrom, quenching of targeted signal molecules instigate the dominance of microbial species bearing non-targeted quorum sensing mechanism.					
36610508	1	5	theme	quenching	198:206	arg1	enzymes					213:219	four quorum quenching (QQ) enzymes	186:219	four quorum quenching (QQ) enzymes	186:219	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes were applied in immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control.					
36610508	4	6	theme	conventional	865:876	arg1	phases					878:883	conventional phases	865:883	conventional phases	865:883	Biocake with low surface coverage was prominent during QQ application compared to conventional phases.					
36610508	4	7	with	Biocake	783:789	arg1	coverage					808:815	low surface coverage	796:815	low surface coverage	796:815	Biocake with low surface coverage was prominent during QQ application compared to conventional phases.					
36610508	6	8	theme	sensing	1208:1214	arg1	mechanism					1216:1224	non-targeted quorum sensing mechanism	1188:1224	non-targeted quorum sensing mechanism	1188:1224	Therefrom, quenching of targeted signal molecules instigate the dominance of microbial species bearing non-targeted quorum sensing mechanism.					
36610508	1	9	theme	QQ	209:210	arg1	enzymes					213:219	four quorum quenching (QQ) enzymes	186:219	four quorum quenching (QQ) enzymes	186:219	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes were applied in immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control.					
36610508	3	10	from	%	741:741	arg1	AnMBR-Hf					759:766	AnMBR-Hf	759:766	AnMBR-Hf	759:766	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	3	10	from	%	741:741	arg1	AnMBR-Si					746:753	AnMBR-Si	746:753	AnMBR-Si	746:753	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	1	11	from	implications	283:294	arg1	foulants					308:315	membrane foulants	299:315	membrane foulants	299:315	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes were applied in immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control.					
36610508	1	11	from	implications	283:294	arg1	taxa					328:331	microbial taxa	318:331	microbial taxa	318:331	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes were applied in immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control.					
36610508	1	11	from	implications	283:294	arg1	control					349:355	biofouling control	338:355	biofouling control	338:355	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes were applied in immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control.					
36610508	5	12	from	points	977:982	arg1	3OC6-					922:926	3OC6-	922:926	3OC6-	922:926	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	12	from	points	977:982	arg1	C6-					929:931	C6-	929:931	C6-	929:931	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	12	from	points	977:982	arg1	C10-HSL					948:954	C10-HSL	948:954	C10-HSL	948:954	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	12	from	points	977:982	arg1	control					905:911	the better control	894:911	the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases	894:995	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	12	from	points	977:982	arg1	AHLs					916:919	AHLs	916:919	AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL)	916:955	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	12	from	points	977:982	arg1	3OC8					934:937	3OC8	934:937	3OC8	934:937	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	12	from	points	977:982	arg1	C8					940:941	C8	940:941	C8	940:941	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	12	from	points	977:982	arg1	AI-2					961:964	AI-2	961:964	AI-2 at various points of QQ phases	961:995	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	2	13	theme	conventional	552:563	arg1	phases					584:589	two conventional and three QQ based phases	548:589	two conventional and three QQ based phases	548:589	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	6	14	theme	quorum	1201:1206	arg1	sensing					1208:1214	non-targeted quorum sensing	1188:1214	non-targeted quorum sensing mechanism	1188:1224	Therefrom, quenching of targeted signal molecules instigate the dominance of microbial species bearing non-targeted quorum sensing mechanism.					
36610508	7	15	theme	transduction	1340:1351	arg1	systems					1353:1359	robust signal transduction systems	1326:1359	robust signal transduction systems	1326:1359	The QQ significantly altered the biofilm-forming community in mixed liquor, while the members with robust signal transduction systems became dominant to counteract the QQ mechanism and were the ultimate cause of biofouling.					
36610508	3	16	theme	synergistic	596:606	arg1	addition					608:615	The synergistic addition	592:615	The synergistic addition of QQ anaerobes in free cells	592:645	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	2	17	theme	based	578:582	arg1	phases					584:589	two conventional and three QQ based phases	548:589	two conventional and three QQ based phases	548:589	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	7	18	theme	robust	1326:1331	arg1	systems					1353:1359	robust signal transduction systems	1326:1359	robust signal transduction systems	1326:1359	The QQ significantly altered the biofilm-forming community in mixed liquor, while the members with robust signal transduction systems became dominant to counteract the QQ mechanism and were the ultimate cause of biofouling.					
36610508	6	19	theme	microbial	1162:1170	arg1	species					1172:1178	microbial species	1162:1178	microbial species bearing non-targeted quorum sensing mechanism	1162:1224	Therefrom, quenching of targeted signal molecules instigate the dominance of microbial species bearing non-targeted quorum sensing mechanism.					
36610508	5	20	theme	QQ	1002:1003	arg1	consortium					1005:1014	the QQ consortium	998:1014	the QQ consortium	998:1014	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	3	21	theme	free	636:639	arg1	cells					641:645	free cells	636:645	free cells	636:645	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	8	22	theme	improved	1455:1462	arg1	content					1472:1478	The improved methane content	1451:1478	The improved methane content in biogas	1451:1488	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	0	23	theme	lab-scale	62:70	arg1	bioreactors					91:101	lab-scale anaerobic membrane bioreactors	62:101	lab-scale anaerobic membrane bioreactors	62:101	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	1	24	theme	biofouling	338:347	arg1	control					349:355	biofouling control	338:355	biofouling control	338:355	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes were applied in immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control.					
36610508	3	25	theme	anaerobes	623:631	arg1	addition					608:615	The synergistic addition	592:615	The synergistic addition of QQ anaerobes in free cells	592:645	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	3	25	theme	anaerobes	623:631	arg1	form					667:670	the immobilized form	651:670	the immobilized form	651:670	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	0	26	from	potential	19:27	arg1	bioreactors					91:101	lab-scale anaerobic membrane bioreactors	62:101	lab-scale anaerobic membrane bioreactors	62:101	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	0	27	theme	membrane	82:89	arg1	bioreactors					91:101	lab-scale anaerobic membrane bioreactors	62:101	lab-scale anaerobic membrane bioreactors	62:101	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	5	28	theme	AHLs	916:919	arg1	control					905:911	the better control	894:911	the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases	894:995	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	29	theme	low	1037:1039	arg1	concentration					1041:1053	a low concentration	1035:1053	a low concentration of signals for longer period	1035:1082	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	0	30	from	anaerobes	49:57	arg1	bioreactors					91:101	lab-scale anaerobic membrane bioreactors	62:101	lab-scale anaerobic membrane bioreactors	62:101	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	0	31	theme	anaerobic	72:80	arg1	bioreactors					91:101	lab-scale anaerobic membrane bioreactors	62:101	lab-scale anaerobic membrane bioreactors	62:101	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	8	32	theme	QQ	1535:1536	arg1	phases					1538:1543	QQ phases	1535:1543	QQ phases	1535:1543	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	8	33	from	content	1472:1478	arg1	biogas					1483:1488	biogas	1483:1488	biogas	1483:1488	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	7	34	theme	mixed	1289:1293	arg1	liquor					1295:1300	mixed liquor	1289:1300	mixed liquor	1289:1300	The QQ significantly altered the biofilm-forming community in mixed liquor, while the members with robust signal transduction systems became dominant to counteract the QQ mechanism and were the ultimate cause of biofouling.					
36610508	3	35	theme	immobilized	655:665	arg1	form					667:670	the immobilized form	651:670	the immobilized form	651:670	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	2	36	theme	immobilizing	427:438	arg1	media					440:444	different immobilizing media	417:444	different immobilizing media	417:444	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	0	37	theme	quorum	32:37	arg1	anaerobes					49:57	quorum quenching anaerobes	32:57	quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors	32:101	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	1	38	theme	microbial	318:326	arg1	taxa					328:331	microbial taxa	318:331	microbial taxa	318:331	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes were applied in immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control.					
36610508	8	39	from	composition	1516:1526	arg1	biogas					1483:1488	biogas	1483:1488	biogas	1483:1488	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	4	40	theme	QQ	838:839	arg1	application					841:851	QQ application	838:851	QQ application	838:851	Biocake with low surface coverage was prominent during QQ application compared to conventional phases.					
36610508	0	41	theme	anaerobes	49:57	arg1	potential					19:27	control potential	11:27	control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors	11:101	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	5	42	theme	phases	990:995	arg1	points					977:982	various points	969:982	various points of QQ phases	969:995	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	8	43	theme	exogenous	1575:1583	arg1	option					1623:1628	the most viable option	1607:1628	the most viable option for long-term AnMBR operation	1607:1658	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	8	43	theme	exogenous	1575:1583	arg1	synergism					1562:1570	the synergism	1558:1570	the synergism of exogenous and immobilized QQ	1558:1602	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	3	44	theme	membrane	686:693	arg1	operation					706:714	the membrane filtration operation	682:714	the membrane filtration operation	682:714	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	4	45	theme	low	796:798	arg1	coverage					808:815	low surface coverage	796:815	low surface coverage	796:815	Biocake with low surface coverage was prominent during QQ application compared to conventional phases.					
36610508	8	46	theme	long-term	1634:1642	arg1	operation					1650:1658	long-term AnMBR operation	1634:1658	long-term AnMBR operation	1634:1658	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	2	47	with	bioreactors	391:401	arg1	media					440:444	different immobilizing media	417:444	different immobilizing media	417:444	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	5	48	theme	AI-2	961:964	arg1	control					905:911	the better control	894:911	the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases	894:995	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	8	49	theme	immobilized	1589:1599	arg1	QQ					1601:1602	immobilized QQ	1589:1602	immobilized QQ	1589:1602	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	3	50	from	form	667:670	arg1	cells					641:645	free cells	636:645	free cells	636:645	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	5	51	theme	various	969:975	arg1	points					977:982	various points	969:982	various points of QQ phases	969:995	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	2	52	theme	anaerobic	372:380	arg1	AnMBRs					404:409	AnMBRs	404:409	AnMBRs	404:409	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	2	52	theme	anaerobic	372:380	arg1	bioreactors					391:401	Two identical anaerobic membrane bioreactors	358:401	Two identical anaerobic membrane bioreactors (AnMBRs)	358:410	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	8	53	theme	most	1611:1614	arg1	option					1623:1628	the most viable option	1607:1628	the most viable option for long-term AnMBR operation	1607:1658	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	8	53	theme	most	1611:1614	arg1	synergism					1562:1570	the synergism	1558:1570	the synergism of exogenous and immobilized QQ	1558:1602	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	6	54	theme	molecules	1125:1133	arg1	quenching					1096:1104	quenching	1096:1104	quenching	1096:1104	Therefrom, quenching of targeted signal molecules instigate the dominance of microbial species bearing non-targeted quorum sensing mechanism.					
36610508	6	54	theme	molecules	1125:1133	arg1	Therefrom					1085:1093	Therefrom	1085:1093	Therefrom	1085:1093	Therefrom, quenching of targeted signal molecules instigate the dominance of microbial species bearing non-targeted quorum sensing mechanism.					
36610508	1	55	theme	quorum	191:196	arg1	enzymes					213:219	four quorum quenching (QQ) enzymes	186:219	four quorum quenching (QQ) enzymes	186:219	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes were applied in immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control.					
36610508	7	56	theme	ultimate	1421:1428	arg1	cause					1430:1434	the ultimate cause	1417:1434	the ultimate cause of biofouling	1417:1448	The QQ significantly altered the biofilm-forming community in mixed liquor, while the members with robust signal transduction systems became dominant to counteract the QQ mechanism and were the ultimate cause of biofouling.					
36610508	6	57	theme	targeted	1109:1116	arg1	molecules					1125:1133	targeted signal molecules	1109:1133	targeted signal molecules	1109:1133	Therefrom, quenching of targeted signal molecules instigate the dominance of microbial species bearing non-targeted quorum sensing mechanism.					
36610508	3	58	from	%	727:727	arg1	AnMBR-Hf					759:766	AnMBR-Hf	759:766	AnMBR-Hf	759:766	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	3	58	from	%	727:727	arg1	AnMBR-Si					746:753	AnMBR-Si	746:753	AnMBR-Si	746:753	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	7	59	theme	QQ	1395:1396	arg1	mechanism					1398:1406	the QQ mechanism	1391:1406	the QQ mechanism	1391:1406	The QQ significantly altered the biofilm-forming community in mixed liquor, while the members with robust signal transduction systems became dominant to counteract the QQ mechanism and were the ultimate cause of biofouling.					
36610508	0	60	from	bioreactors	91:101	arg1	potential					19:27	control potential	11:27	control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors	11:101	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	1	61	theme	isolated	158:165	arg1	anaerobes					167:175	Indigenously isolated anaerobes	145:175	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes	145:219	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes were applied in immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control.					
36610508	6	62	theme	non-targeted	1188:1199	arg1	sensing					1208:1214	non-targeted quorum sensing	1188:1214	non-targeted quorum sensing mechanism	1188:1224	Therefrom, quenching of targeted signal molecules instigate the dominance of microbial species bearing non-targeted quorum sensing mechanism.					
36610508	6	63	theme	species	1172:1178	arg1	dominance					1149:1157	the dominance	1145:1157	the dominance of microbial species bearing non-targeted quorum sensing mechanism	1145:1224	Therefrom, quenching of targeted signal molecules instigate the dominance of microbial species bearing non-targeted quorum sensing mechanism.					
36610508	8	64	theme	methane	1464:1470	arg1	content					1472:1478	The improved methane content	1451:1478	The improved methane content in biogas	1451:1488	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	7	65	theme	signal	1333:1338	arg1	systems					1353:1359	robust signal transduction systems	1326:1359	robust signal transduction systems	1326:1359	The QQ significantly altered the biofilm-forming community in mixed liquor, while the members with robust signal transduction systems became dominant to counteract the QQ mechanism and were the ultimate cause of biofouling.					
36610508	7	66	with	members	1313:1319	arg1	systems					1353:1359	robust signal transduction systems	1326:1359	robust signal transduction systems	1326:1359	The QQ significantly altered the biofilm-forming community in mixed liquor, while the members with robust signal transduction systems became dominant to counteract the QQ mechanism and were the ultimate cause of biofouling.					
36610508	3	67	theme	QQ	620:621	arg1	anaerobes					623:631	QQ anaerobes	620:631	QQ anaerobes	620:631	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	5	68	theme	better	898:903	arg1	control					905:911	the better control	894:911	the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases	894:995	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	69	theme	longer	1070:1075	arg1	period					1077:1082	longer period	1070:1082	longer period	1070:1082	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	0	70	theme	microbial	125:133	arg1	dynamics					135:142	microbial dynamics	125:142	microbial dynamics	125:142	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	0	71	theme	Foulants	104:111	arg1	profile					113:119	Foulants profile	104:119	Foulants profile	104:119	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	8	72	theme	methanogens	1504:1514	arg1	composition					1516:1526	increased methanogens composition	1494:1526	increased methanogens composition during QQ phases	1494:1543	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	2	73	dep	silica-alginate	452:466	arg1	i.e.					447:450	i.e.	447:450	i.e.	447:450	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	5	74	from	control	905:911	arg1	points					977:982	various points	969:982	various points of QQ phases	969:995	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	0	75	theme	control	11:17	arg1	potential					19:27	control potential	11:27	control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors	11:101	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	5	76	theme	signals	1058:1064	arg1	concentration					1041:1053	a low concentration	1035:1053	a low concentration of signals for longer period	1035:1082	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	8	77	theme	increased	1494:1502	arg1	composition					1516:1526	increased methanogens composition	1494:1526	increased methanogens composition during QQ phases	1494:1543	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	5	78	dep	AHLs	916:919	arg1	3OC6-					922:926	3OC6-	922:926	3OC6-	922:926	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	78	dep	AHLs	916:919	arg1	C6-					929:931	C6-	929:931	C6-	929:931	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	78	dep	AHLs	916:919	arg1	C10-HSL					948:954	C10-HSL	948:954	C10-HSL	948:954	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	78	dep	AHLs	916:919	arg1	AHLs					916:919	AHLs	916:919	AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL)	916:955	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	78	dep	AHLs	916:919	arg1	3OC8					934:937	3OC8	934:937	3OC8	934:937	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	5	78	dep	AHLs	916:919	arg1	C8					940:941	C8	940:941	C8	940:941	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	1	79	theme	membrane	299:306	arg1	foulants					308:315	membrane foulants	299:315	membrane foulants	299:315	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes were applied in immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control.					
36610508	3	80	from	addition	608:615	arg1	cells					641:645	free cells	636:645	free cells	636:645	The synergistic addition of QQ anaerobes in free cells and the immobilized form prolonged the membrane filtration operation by 172 ± 29% and 284 ± 12% in AnMBR-Si and AnMBR-Hf, respectively.					
36610508	0	81	theme	quenching	39:47	arg1	anaerobes					49:57	quorum quenching anaerobes	32:57	quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors	32:101	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	7	82	theme	biofilm-forming	1260:1274	arg1	community					1276:1284	the biofilm-forming community	1256:1284	the biofilm-forming community in mixed liquor	1256:1300	The QQ significantly altered the biofilm-forming community in mixed liquor, while the members with robust signal transduction systems became dominant to counteract the QQ mechanism and were the ultimate cause of biofouling.					
36610508	2	83	theme	hollow	483:488	arg1	AnMBR-Hf					506:513	AnMBR-Hf	506:513	AnMBR-Hf	506:513	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	2	83	theme	hollow	483:488	arg1	fiber-alginate					490:503	hollow fiber-alginate	483:503	hollow fiber-alginate (AnMBR-Hf)	483:514	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	2	84	theme	different	417:425	arg1	media					440:444	different immobilizing media	417:444	different immobilizing media	417:444	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	5	85	theme	QQ	987:988	arg1	phases					990:995	QQ phases	987:995	QQ phases	987:995	Despite the better control of AHLs (3OC6-, C6-, 3OC8, C8, and C10-HSL) and AI-2 at various points of QQ phases, the QQ consortium could not maintain a low concentration of signals for longer period.					
36610508	7	86	from	community	1276:1284	arg1	liquor					1295:1300	mixed liquor	1289:1300	mixed liquor	1289:1300	The QQ significantly altered the biofilm-forming community in mixed liquor, while the members with robust signal transduction systems became dominant to counteract the QQ mechanism and were the ultimate cause of biofouling.					
36610508	8	87	theme	AnMBR	1644:1648	arg1	operation					1650:1658	long-term AnMBR operation	1634:1658	long-term AnMBR operation	1634:1658	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	0	88	dep	Biofouling	0:9	arg1	profile					113:119	Foulants profile	104:119	Foulants profile	104:119	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	0	88	dep	Biofouling	0:9	arg1	dynamics					135:142	microbial dynamics	125:142	microbial dynamics	125:142	Biofouling control potential of quorum quenching anaerobes in lab-scale anaerobic membrane bioreactors: Foulants profile and microbial dynamics.					
36610508	8	89	theme	QQ	1601:1602	arg1	option					1623:1628	the most viable option	1607:1628	the most viable option for long-term AnMBR operation	1607:1658	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	8	89	theme	QQ	1601:1602	arg1	synergism					1562:1570	the synergism	1558:1570	the synergism of exogenous and immobilized QQ	1558:1602	The improved methane content in biogas and increased methanogens composition during QQ phases demonstrated the synergism of exogenous and immobilized QQ as the most viable option for long-term AnMBR operation.					
36610508	1	90	theme	immobilized-	237:248	arg1	forms					267:271	immobilized- and bioaugmented forms	237:271	immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control	237:355	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes were applied in immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control.					
36610508	2	91	dep	based	578:582	arg1	QQ					575:576	three QQ	569:576	three QQ	569:576	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	4	92	theme	surface	800:806	arg1	coverage					808:815	low surface coverage	796:815	low surface coverage	796:815	Biocake with low surface coverage was prominent during QQ application compared to conventional phases.					
36610508	2	93	theme	membrane	382:389	arg1	AnMBRs					404:409	AnMBRs	404:409	AnMBRs	404:409	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	2	93	theme	membrane	382:389	arg1	bioreactors					391:401	Two identical anaerobic membrane bioreactors	358:401	Two identical anaerobic membrane bioreactors (AnMBRs)	358:410	Two identical anaerobic membrane bioreactors (AnMBRs) with different immobilizing media, i.e. silica-alginate (AnMBR-Si) and hollow fiber-alginate (AnMBR-Hf), were sequentially operated for two conventional and three QQ based phases.					
36610508	1	94	theme	bioaugmented	254:265	arg1	forms					267:271	immobilized- and bioaugmented forms	237:271	immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control	237:355	Indigenously isolated anaerobes encoding four quorum quenching (QQ) enzymes were applied in immobilized- and bioaugmented forms for their implications on membrane foulants, microbial taxa, and biofouling control.					
36306896	0	0	theme	cotton	102:107	arg1	fabric					109:114	carboxymethylated cotton fabric	84:114	carboxymethylated cotton fabric for hemostasis and infected wound treatment	84:158	Composite hydrogel based oxidated sodium carboxymethyl cellulose and gelatin loaded carboxymethylated cotton fabric for hemostasis and infected wound treatment.					
36306896	1	1	theme	wound	178:182	arg1	dressings					184:192	The fabric-based wound dressings	161:192	The fabric-based wound dressings	161:192	The fabric-based wound dressings are hard to maintain a moist environment for wound healing while the hemostatic property and gas permeability of some hydrogel-based wound dressings are not ideal.					
36306896	3	2	theme	mechanical	773:782	arg1	property					784:791	good mechanical property	768:791	good mechanical property	768:791	In this manner, the composite dressing not only maintained the advantages of hydrogels, but also inherited good mechanical property, hemostatic property, and gas permeability from the base fabric.					
36306896	1	3	theme	hemostatic	263:272	arg1	property					274:281	hemostatic property	263:281	hemostatic property	263:281	The fabric-based wound dressings are hard to maintain a moist environment for wound healing while the hemostatic property and gas permeability of some hydrogel-based wound dressings are not ideal.					
36306896	0	4	theme	carboxymethylated	84:100	arg1	fabric					109:114	carboxymethylated cotton fabric	84:114	carboxymethylated cotton fabric for hemostasis and infected wound treatment	84:158	Composite hydrogel based oxidated sodium carboxymethyl cellulose and gelatin loaded carboxymethylated cotton fabric for hemostasis and infected wound treatment.					
36306896	5	5	theme	Ag	1095:1096	arg1	nanoparticles					1098:1110	dopamine and Ag nanoparticles	1082:1110	dopamine and Ag nanoparticles	1082:1110	To obtain multifunctional hydrogel, sodium carboxymethylcellulose was oxidated to introduce aldehyde groups to form Schiff base with amino groups in gelatin, besides, dopamine and Ag nanoparticles were introduced to endow the hydrogel with antioxidant property and antibacterial activity.					
36306896	6	6	theme	MCF	1262:1264	arg1	side					1254:1257	one side	1250:1257	one side of MCF	1250:1264	The multifunctional hydrogel was printed onto one side of MCF, subsequently, the deposition of paraffin made the other side of this dressing become hydrophobic.					
36306896	5	7	from	groups	1054:1059	arg1	gelatin					1064:1070	gelatin	1064:1070	gelatin	1064:1070	To obtain multifunctional hydrogel, sodium carboxymethylcellulose was oxidated to introduce aldehyde groups to form Schiff base with amino groups in gelatin, besides, dopamine and Ag nanoparticles were introduced to endow the hydrogel with antioxidant property and antibacterial activity.					
36306896	2	8	theme	fabric	635:640	arg1	side					618:621	the other side	608:621	the other side of the base fabric	608:640	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	2	9	dep	put	375:377	arg1	1					438:438	1	438:438	1	438:438	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	3	10	theme	base	845:848	arg1	fabric					850:855	the base fabric	841:855	the base fabric	841:855	In this manner, the composite dressing not only maintained the advantages of hydrogels, but also inherited good mechanical property, hemostatic property, and gas permeability from the base fabric.					
36306896	5	11	theme	antibacterial	1180:1192	arg1	activity					1194:1201	antibacterial activity	1180:1201	antibacterial activity	1180:1201	To obtain multifunctional hydrogel, sodium carboxymethylcellulose was oxidated to introduce aldehyde groups to form Schiff base with amino groups in gelatin, besides, dopamine and Ag nanoparticles were introduced to endow the hydrogel with antioxidant property and antibacterial activity.					
36306896	2	12	theme	base	630:633	arg1	fabric					635:640	the base fabric	626:640	the base fabric	626:640	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	5	13	theme	multifunctional	925:939	arg1	hydrogel					941:948	multifunctional hydrogel	925:948	multifunctional hydrogel	925:948	To obtain multifunctional hydrogel, sodium carboxymethylcellulose was oxidated to introduce aldehyde groups to form Schiff base with amino groups in gelatin, besides, dopamine and Ag nanoparticles were introduced to endow the hydrogel with antioxidant property and antibacterial activity.					
36306896	1	14	theme	gas	287:289	arg1	permeability					291:302	gas permeability	287:302	gas permeability	287:302	The fabric-based wound dressings are hard to maintain a moist environment for wound healing while the hemostatic property and gas permeability of some hydrogel-based wound dressings are not ideal.					
36306896	7	15	theme	wound	1437:1441	arg1	healing					1443:1449	wound healing	1437:1449	wound healing	1437:1449	The good performance of the obtained dressing in hemostatic process and wound healing demonstrated its potential in the field of wound treatment.					
36306896	2	16	theme	base	552:555	arg1	fabric					557:562	the base fabric	548:562	the base fabric	548:562	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	2	17	theme	wound	422:426	arg1	dressing					428:435	checkerboard-pattern wound dressing	401:435	checkerboard-pattern wound dressing	401:435	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	3	18	theme	hemostatic	794:803	arg1	property					805:812	hemostatic property	794:812	hemostatic property	794:812	In this manner, the composite dressing not only maintained the advantages of hydrogels, but also inherited good mechanical property, hemostatic property, and gas permeability from the base fabric.					
36306896	3	19	theme	composite	681:689	arg1	dressing					691:698	the composite dressing	677:698	the composite dressing	677:698	In this manner, the composite dressing not only maintained the advantages of hydrogels, but also inherited good mechanical property, hemostatic property, and gas permeability from the base fabric.					
36306896	6	20	theme	other	1317:1321	arg1	side					1323:1326	the other side	1313:1326	the other side of this dressing	1313:1343	The multifunctional hydrogel was printed onto one side of MCF, subsequently, the deposition of paraffin made the other side of this dressing become hydrophobic.					
36306896	2	21	theme	base	455:458	arg1	fabric					460:465	the base fabric	451:465	the base fabric	451:465	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	2	22	theme	checkerboard-pattern	401:420	arg1	dressing					428:435	checkerboard-pattern wound dressing	401:435	checkerboard-pattern wound dressing	401:435	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	0	23	theme	hydrogel	10:17	arg1	cellulose					55:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose	0:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose	0:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose and gelatin loaded carboxymethylated cotton fabric for hemostasis and infected wound treatment.					
36306896	5	24	theme	antioxidant	1155:1165	arg1	property					1167:1174	antioxidant property	1155:1174	antioxidant property	1155:1174	To obtain multifunctional hydrogel, sodium carboxymethylcellulose was oxidated to introduce aldehyde groups to form Schiff base with amino groups in gelatin, besides, dopamine and Ag nanoparticles were introduced to endow the hydrogel with antioxidant property and antibacterial activity.					
36306896	2	25	theme	other	612:616	arg1	side					618:621	the other side	608:621	the other side of the base fabric	608:640	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	2	26	dep	form	567:570	arg1	2					493:493	2	493:493	2	493:493	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	2	27	theme	checkerboard	572:583	arg1	patterns					585:592	checkerboard patterns	572:592	checkerboard patterns	572:592	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	0	28	theme	Composite	0:8	arg1	cellulose					55:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose	0:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose	0:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose and gelatin loaded carboxymethylated cotton fabric for hemostasis and infected wound treatment.					
36306896	0	29	theme	oxidated	25:32	arg1	cellulose					55:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose	0:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose	0:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose and gelatin loaded carboxymethylated cotton fabric for hemostasis and infected wound treatment.					
36306896	0	30	theme	wound	144:148	arg1	treatment					150:158	infected wound treatment	135:158	infected wound treatment	135:158	Composite hydrogel based oxidated sodium carboxymethyl cellulose and gelatin loaded carboxymethylated cotton fabric for hemostasis and infected wound treatment.					
36306896	6	31	theme	dressing	1336:1343	arg1	side					1323:1326	the other side	1313:1326	the other side of this dressing	1313:1343	The multifunctional hydrogel was printed onto one side of MCF, subsequently, the deposition of paraffin made the other side of this dressing become hydrophobic.					
36306896	0	32	theme	based	19:23	arg1	cellulose					55:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose	0:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose	0:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose and gelatin loaded carboxymethylated cotton fabric for hemostasis and infected wound treatment.					
36306896	0	33	theme	infected	135:142	arg1	treatment					150:158	infected wound treatment	135:158	infected wound treatment	135:158	Composite hydrogel based oxidated sodium carboxymethyl cellulose and gelatin loaded carboxymethylated cotton fabric for hemostasis and infected wound treatment.					
36306896	7	34	theme	good	1369:1372	arg1	performance					1374:1384	The good performance	1365:1384	The good performance of the obtained dressing in hemostatic process and wound healing	1365:1449	The good performance of the obtained dressing in hemostatic process and wound healing demonstrated its potential in the field of wound treatment.					
36306896	1	35	theme	moist	217:221	arg1	environment					223:233	a moist environment	215:233	a moist environment for wound healing	215:251	The fabric-based wound dressings are hard to maintain a moist environment for wound healing while the hemostatic property and gas permeability of some hydrogel-based wound dressings are not ideal.					
36306896	0	36	theme	carboxymethyl	41:53	arg1	cellulose					55:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose	0:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose	0:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose and gelatin loaded carboxymethylated cotton fabric for hemostasis and infected wound treatment.					
36306896	1	37	theme	hydrogel-based	312:325	arg1	dressings					333:341	some hydrogel-based wound dressings	307:341	some hydrogel-based wound dressings	307:341	The fabric-based wound dressings are hard to maintain a moist environment for wound healing while the hemostatic property and gas permeability of some hydrogel-based wound dressings are not ideal.					
36306896	6	38	theme	paraffin	1299:1306	arg1	deposition					1285:1294	the deposition	1281:1294	the deposition of paraffin	1281:1306	The multifunctional hydrogel was printed onto one side of MCF, subsequently, the deposition of paraffin made the other side of this dressing become hydrophobic.					
36306896	5	39	theme	amino	1048:1052	arg1	groups					1054:1059	amino groups	1048:1059	amino groups in gelatin	1048:1070	To obtain multifunctional hydrogel, sodium carboxymethylcellulose was oxidated to introduce aldehyde groups to form Schiff base with amino groups in gelatin, besides, dopamine and Ag nanoparticles were introduced to endow the hydrogel with antioxidant property and antibacterial activity.					
36306896	0	40	theme	sodium	34:39	arg1	cellulose					55:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose	0:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose	0:63	Composite hydrogel based oxidated sodium carboxymethyl cellulose and gelatin loaded carboxymethylated cotton fabric for hemostasis and infected wound treatment.					
36306896	1	41	theme	wound	327:331	arg1	dressings					333:341	some hydrogel-based wound dressings	307:341	some hydrogel-based wound dressings	307:341	The fabric-based wound dressings are hard to maintain a moist environment for wound healing while the hemostatic property and gas permeability of some hydrogel-based wound dressings are not ideal.					
36306896	7	42	theme	wound	1494:1498	arg1	treatment					1500:1508	wound treatment	1494:1508	wound treatment	1494:1508	The good performance of the obtained dressing in hemostatic process and wound healing demonstrated its potential in the field of wound treatment.					
36306896	6	43	theme	multifunctional	1208:1222	arg1	hydrogel					1224:1231	The multifunctional hydrogel	1204:1231	The multifunctional hydrogel	1204:1231	The multifunctional hydrogel was printed onto one side of MCF, subsequently, the deposition of paraffin made the other side of this dressing become hydrophobic.					
36306896	2	44	theme	dressing	428:435	arg1	strategy					389:396	a strategy	387:396	a strategy of checkerboard-pattern wound dressing	387:435	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	2	45	theme	hemostatic	472:481	arg1	property					483:490	hemostatic property	472:490	hemostatic property	472:490	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	1	46	theme	dressings	333:341	arg1	property					274:281	hemostatic property	263:281	hemostatic property	263:281	The fabric-based wound dressings are hard to maintain a moist environment for wound healing while the hemostatic property and gas permeability of some hydrogel-based wound dressings are not ideal.					
36306896	1	46	theme	dressings	333:341	arg1	permeability					291:302	gas permeability	287:302	gas permeability	287:302	The fabric-based wound dressings are hard to maintain a moist environment for wound healing while the hemostatic property and gas permeability of some hydrogel-based wound dressings are not ideal.					
36306896	7	47	theme	treatment	1500:1508	arg1	field					1485:1489	the field	1481:1489	the field of wound treatment	1481:1508	The good performance of the obtained dressing in hemostatic process and wound healing demonstrated its potential in the field of wound treatment.					
36306896	5	48	theme	aldehyde	1007:1014	arg1	groups					1016:1021	aldehyde groups	1007:1021	aldehyde groups	1007:1021	To obtain multifunctional hydrogel, sodium carboxymethylcellulose was oxidated to introduce aldehyde groups to form Schiff base with amino groups in gelatin, besides, dopamine and Ag nanoparticles were introduced to endow the hydrogel with antioxidant property and antibacterial activity.					
36306896	7	49	theme	hemostatic	1414:1423	arg1	process					1425:1431	hemostatic process	1414:1431	hemostatic process	1414:1431	The good performance of the obtained dressing in hemostatic process and wound healing demonstrated its potential in the field of wound treatment.					
36306896	7	50	from	potential	1468:1476	arg1	field					1485:1489	the field	1481:1489	the field of wound treatment	1481:1508	The good performance of the obtained dressing in hemostatic process and wound healing demonstrated its potential in the field of wound treatment.					
36306896	3	51	theme	hydrogels	738:746	arg1	advantages					724:733	the advantages	720:733	the advantages of hydrogels	720:746	In this manner, the composite dressing not only maintained the advantages of hydrogels, but also inherited good mechanical property, hemostatic property, and gas permeability from the base fabric.					
36306896	2	52	theme	fabric	557:562	arg1	side					540:543	one side	536:543	one side of the base fabric	536:562	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	2	53	dep	modifying	598:606	arg1	3					595:595	3	595:595	3	595:595	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	4	54	theme	cotton	868:873	arg1	MCF					910:912	MCF	910:912	MCF	910:912	Here, the cotton fabric was carboxymethylated to be MCF.					
36306896	4	54	theme	cotton	868:873	arg1	fabric					875:880	the cotton fabric	864:880	the cotton fabric	864:880	Here, the cotton fabric was carboxymethylated to be MCF.					
36306896	5	55	theme	sodium	951:956	arg1	carboxymethylcellulose					958:979	sodium carboxymethylcellulose	951:979	sodium carboxymethylcellulose	951:979	To obtain multifunctional hydrogel, sodium carboxymethylcellulose was oxidated to introduce aldehyde groups to form Schiff base with amino groups in gelatin, besides, dopamine and Ag nanoparticles were introduced to endow the hydrogel with antioxidant property and antibacterial activity.					
36306896	1	56	theme	wound	239:243	arg1	healing					245:251	wound healing	239:251	wound healing	239:251	The fabric-based wound dressings are hard to maintain a moist environment for wound healing while the hemostatic property and gas permeability of some hydrogel-based wound dressings are not ideal.					
36306896	7	57	theme	obtained	1393:1400	arg1	dressing					1402:1409	the obtained dressing	1389:1409	the obtained dressing	1389:1409	The good performance of the obtained dressing in hemostatic process and wound healing demonstrated its potential in the field of wound treatment.					
36306896	5	58	theme	Schiff	1031:1036	arg1	base					1038:1041	Schiff base	1031:1041	Schiff base	1031:1041	To obtain multifunctional hydrogel, sodium carboxymethylcellulose was oxidated to introduce aldehyde groups to form Schiff base with amino groups in gelatin, besides, dopamine and Ag nanoparticles were introduced to endow the hydrogel with antioxidant property and antibacterial activity.					
36306896	7	59	theme	dressing	1402:1409	arg1	performance					1374:1384	The good performance	1365:1384	The good performance of the obtained dressing in hemostatic process and wound healing	1365:1449	The good performance of the obtained dressing in hemostatic process and wound healing demonstrated its potential in the field of wound treatment.					
36306896	7	60	from	performance	1374:1384	arg1	process					1425:1431	hemostatic process	1414:1431	hemostatic process	1414:1431	The good performance of the obtained dressing in hemostatic process and wound healing demonstrated its potential in the field of wound treatment.					
36306896	7	60	from	performance	1374:1384	arg1	healing					1443:1449	wound healing	1437:1449	wound healing	1437:1449	The good performance of the obtained dressing in hemostatic process and wound healing demonstrated its potential in the field of wound treatment.					
36306896	2	61	theme	multifunctional	505:519	arg1	hydrogels					521:529	printing multifunctional hydrogels	496:529	printing multifunctional hydrogels onto one side of the base fabric	496:562	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	1	62	dep	property	274:281	arg1	the					259:261	the	259:261	the	259:261	The fabric-based wound dressings are hard to maintain a moist environment for wound healing while the hemostatic property and gas permeability of some hydrogel-based wound dressings are not ideal.					
36306896	3	63	theme	gas	819:821	arg1	permeability					823:834	gas permeability	819:834	gas permeability	819:834	In this manner, the composite dressing not only maintained the advantages of hydrogels, but also inherited good mechanical property, hemostatic property, and gas permeability from the base fabric.					
36306896	2	64	theme	printing	496:503	arg1	hydrogels					521:529	printing multifunctional hydrogels	496:529	printing multifunctional hydrogels onto one side of the base fabric	496:562	This study first put forward a strategy of checkerboard-pattern wound dressing: 1) preparing the base fabric with hemostatic property, 2) printing multifunctional hydrogels onto one side of the base fabric to form checkerboard patterns, 3) modifying the other side of the base fabric to be hydrophobic.					
36306896	5	65	theme	dopamine	1082:1089	arg1	nanoparticles					1098:1110	dopamine and Ag nanoparticles	1082:1110	dopamine and Ag nanoparticles	1082:1110	To obtain multifunctional hydrogel, sodium carboxymethylcellulose was oxidated to introduce aldehyde groups to form Schiff base with amino groups in gelatin, besides, dopamine and Ag nanoparticles were introduced to endow the hydrogel with antioxidant property and antibacterial activity.					
36306896	1	66	theme	fabric-based	165:176	arg1	dressings					184:192	The fabric-based wound dressings	161:192	The fabric-based wound dressings	161:192	The fabric-based wound dressings are hard to maintain a moist environment for wound healing while the hemostatic property and gas permeability of some hydrogel-based wound dressings are not ideal.					
36306896	3	67	theme	good	768:771	arg1	property					784:791	good mechanical property	768:791	good mechanical property	768:791	In this manner, the composite dressing not only maintained the advantages of hydrogels, but also inherited good mechanical property, hemostatic property, and gas permeability from the base fabric.					
37225584	3	0	theme	separation	643:652	arg1	removal					608:614	the simultaneous removal	591:614	the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey	591:682	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	2	1	theme	acid	330:333	arg1	recovery					335:342	Lactic acid recovery	323:342	Lactic acid recovery from fermentation broth without precipitation	323:388	Lactic acid recovery from fermentation broth without precipitation has been studied by numerous processes.					
37225584	5	2	theme	lactic	1081:1086	arg1	rejection					1093:1101	The experimental lactic acid rejection	1064:1101	The experimental lactic acid rejection	1064:1101	The experimental lactic acid rejection was evaluated at varying feed concentration, pressure, temperature, and flow rate.					
37225584	4	3	theme	lactic	962:967	arg1	rejection					974:982	a lactic acid rejection	960:982	a lactic acid rejection lower than 37%	960:997	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	4	4	theme	NF	799:800	arg1	type					803:806	composite nanofiltration (NF) type	773:806	the thin film composite nanofiltration (NF) type	759:806	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	3	5	theme	sweet	673:677	arg1	whey					679:682	the acidified sweet whey	659:682	the acidified sweet whey	659:682	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	3	6	theme	acid	516:519	arg1	rejection					521:529	a moderate lactic acid rejection	498:529	a moderate lactic acid rejection	498:529	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	3	7	dep	40	565:566	arg1	to					562:563	to	562:563	to	562:563	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	3	8	from	whey	679:682	arg1	removal					608:614	the simultaneous removal	591:614	the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey	591:682	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	4	9	theme	low	858:860	arg1	point					874:878	low isoelectric point	858:878	low isoelectric point	858:878	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	3	10	theme	cheese	700:705	arg1	production					707:716	mozzarella cheese production	689:716	mozzarella cheese production	689:716	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	1	11	theme	technology	188:197	arg1	integration					164:174	the integration	160:174	the integration of membrane technology in the dairy industry	160:219	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	7	12	theme	model	1747:1751	arg1	prediction					1753:1762	the model prediction	1743:1762	the model prediction	1743:1762	The results obtained in this work open the way for the up-scaling of membrane technology on the valorization of dairy effluents by simplifying the operation process and the model prediction and the choice of the membrane.					
37225584	6	13	theme	NF	1304:1305	arg1	membrane					1307:1314	this NF membrane	1299:1314	this NF membrane	1299:1314	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	4	14	theme	high	836:839	arg1	charge					850:855	its high negative charge	832:855	its high negative charge	832:855	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	4	15	theme	separation	1046:1055	arg1	steps					1057:1061	additional separation steps	1035:1061	additional separation steps	1035:1061	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	3	16	theme	single	723:728	arg1	stage					730:734	a single stage	721:734	a single stage	721:734	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	6	17	theme	parameter	1462:1470	arg1	values					1472:1477	the parameter values	1458:1477	the parameter values	1458:1477	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	6	18	theme	×	1511:1511	arg1	bar-1					1513:1517	× h-1 × bar-1	1505:1517	× h-1 × bar-1	1505:1517	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	1	19	from	integration	164:174	arg1	industry					212:219	the dairy industry	202:219	the dairy industry	202:219	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	1	20	theme	lactic	129:134	arg1	acid					136:139	lactic acid	129:139	lactic acid	129:139	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	7	21	from	up-scaling	1629:1638	arg1	valorization					1670:1681	the valorization	1666:1681	the valorization of dairy effluents	1666:1700	The results obtained in this work open the way for the up-scaling of membrane technology on the valorization of dairy effluents by simplifying the operation process and the model prediction and the choice of the membrane.					
37225584	6	22	theme	×	1505:1505	arg1	bar-1					1513:1517	× h-1 × bar-1	1505:1517	× h-1 × bar-1	1505:1517	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	0	23	theme	acidified	50:58	arg1	cheese					60:65	acidified cheese	50:65	acidified cheese	50:65	Modeling of lactic acid rejection from lactose in acidified cheese whey by nanofiltration.					
37225584	1	24	theme	waste	305:309	arg1	generation					311:320	waste generation	305:320	waste generation	305:320	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	6	25	from	conditions	1268:1277	arg1	negligible					1231:1240	negligible	1231:1240	negligible	1231:1240	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	6	25	from	conditions	1268:1277	arg1	degree					1206:1211	the dissociation degree	1189:1211	the dissociation degree of lactic acid	1189:1226	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	0	26	from	Modeling	0:7	arg1	lactose					39:45	lactose	39:45	lactose	39:45	Modeling of lactic acid rejection from lactose in acidified cheese whey by nanofiltration.					
37225584	0	26	from	Modeling	0:7	arg1	cheese					60:65	acidified cheese	50:65	acidified cheese	50:65	Modeling of lactic acid rejection from lactose in acidified cheese whey by nanofiltration.					
37225584	6	27	theme	L	1497:1497	arg1	m-2					1501:1503	3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1	1485:1550	m-2	1501:1503	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	4	28	theme	ion	894:896	arg1	rejection					898:906	divalent ion rejection	885:906	divalent ion rejection	885:906	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	6	29	theme	×	1540:1540	arg1	m-2					1542:1544	15.06 ± 3.17 L × m-2	1525:1544	15.06 ± 3.17 L × m-2	1525:1544	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	6	30	theme	lactic	1216:1221	arg1	acid					1223:1226	lactic acid	1216:1226	lactic acid	1216:1226	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	6	31	from	negligible	1231:1240	arg1	conditions					1268:1277	industrially simulated conditions	1245:1277	industrially simulated conditions	1245:1277	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	6	32	theme	±	1490:1490	arg1	m-2					1501:1503	3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1	1485:1550	m-2	1501:1503	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	3	33	theme	mozzarella	689:698	arg1	production					707:716	mozzarella cheese production	689:716	mozzarella cheese production	689:716	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	6	34	theme	membrane	1307:1314	arg1	performance					1284:1294	the performance	1280:1294	the performance of this NF membrane	1280:1314	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	7	35	theme	dairy	1686:1690	arg1	effluents					1692:1700	dairy effluents	1686:1700	dairy effluents	1686:1700	The results obtained in this work open the way for the up-scaling of membrane technology on the valorization of dairy effluents by simplifying the operation process and the model prediction and the choice of the membrane.					
37225584	2	36	theme	numerous	410:417	arg1	processes					419:427	numerous processes	410:427	numerous processes	410:427	Lactic acid recovery from fermentation broth without precipitation has been studied by numerous processes.					
37225584	0	37	theme	lactic	12:17	arg1	rejection					24:32	lactic acid rejection	12:32	lactic acid rejection from lactose	12:45	Modeling of lactic acid rejection from lactose in acidified cheese whey by nanofiltration.					
37225584	6	38	dep	m-2	1501:1503	arg1	bar-1					1513:1517	× h-1 × bar-1	1505:1517	× h-1 × bar-1	1505:1517	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	4	39	theme	film	768:771	arg1	membrane					747:754	The AFC30 membrane	737:754	The AFC30 membrane of the thin film composite nanofiltration (NF) type	737:806	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	0	40	theme	rejection	24:32	arg1	Modeling					0:7	Modeling	0:7	Modeling of lactic acid rejection from lactose in acidified cheese	0:65	Modeling of lactic acid rejection from lactose in acidified cheese whey by nanofiltration.					
37225584	1	41	theme	amounts	280:286	arg1	use					267:269	the use	263:269	the use of large amounts of chemicals and waste generation	263:320	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	6	42	theme	0.45	1561:1564	arg1	±					1566:1566	±	1566:1566	±	1566:1566	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	3	43	theme	simultaneous	595:606	arg1	removal					608:614	the simultaneous removal	591:614	the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey	591:682	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	4	44	theme	nanofiltration	783:796	arg1	type					803:806	composite nanofiltration (NF) type	773:806	the thin film composite nanofiltration (NF) type	759:806	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	3	45	theme	high	471:474	arg1	rejection					484:492	high lactose rejection	471:492	high lactose rejection	471:492	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	1	46	theme	chemicals	291:299	arg1	chemicals					291:299	chemicals	291:299	chemicals	291:299	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	1	46	theme	chemicals	291:299	arg1	generation					311:320	waste generation	305:320	waste generation	305:320	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	1	46	theme	chemicals	291:299	arg1	amounts					280:286	large amounts	274:286	large amounts of chemicals and waste generation	274:320	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	7	47	theme	membrane	1643:1650	arg1	technology					1652:1661	membrane technology	1643:1661	membrane technology	1643:1661	The results obtained in this work open the way for the up-scaling of membrane technology on the valorization of dairy effluents by simplifying the operation process and the model prediction and the choice of the membrane.					
37225584	4	48	theme	lower	984:988	arg1	rejection					974:982	a lactic acid rejection	960:982	a lactic acid rejection lower than 37%	960:997	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	3	49	theme	lactose	635:641	arg1	separation					643:652	lactose separation	635:652	lactose separation	635:652	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	1	50	theme	dairy	206:210	arg1	industry					212:219	the dairy industry	202:219	the dairy industry	202:219	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	3	51	theme	acid	626:629	arg1	removal					608:614	the simultaneous removal	591:614	the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey	591:682	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	6	52	theme	thermodynamic	1350:1362	arg1	Kedem-Katchalsky					1364:1379	the irreversible thermodynamic Kedem-Katchalsky	1333:1379	the irreversible thermodynamic Kedem-Katchalsky	1333:1379	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	3	53	theme	acidified	663:671	arg1	whey					679:682	the acidified sweet whey	659:682	the acidified sweet whey	659:682	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	5	54	theme	experimental	1068:1079	arg1	rejection					1093:1101	The experimental lactic acid rejection	1064:1101	The experimental lactic acid rejection	1064:1101	The experimental lactic acid rejection was evaluated at varying feed concentration, pressure, temperature, and flow rate.					
37225584	4	55	theme	acid	969:972	arg1	rejection					974:982	a lactic acid rejection	960:982	a lactic acid rejection lower than 37%	960:997	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	5	56	theme	acid	1088:1091	arg1	rejection					1093:1101	The experimental lactic acid rejection	1064:1101	The experimental lactic acid rejection	1064:1101	The experimental lactic acid rejection was evaluated at varying feed concentration, pressure, temperature, and flow rate.					
37225584	2	57	theme	Lactic	323:328	arg1	recovery					335:342	Lactic acid recovery	323:342	Lactic acid recovery from fermentation broth without precipitation	323:388	Lactic acid recovery from fermentation broth without precipitation has been studied by numerous processes.					
37225584	3	58	with	membrane	457:464	arg1	rejection					521:529	a moderate lactic acid rejection	498:529	a moderate lactic acid rejection	498:529	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	3	58	with	membrane	457:464	arg1	rejection					484:492	high lactose rejection	471:492	high lactose rejection	471:492	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	3	59	theme	lactic	509:514	arg1	acid					516:519	a moderate lactic acid	498:519	a moderate lactic acid rejection	498:529	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	7	60	theme	membrane	1786:1793	arg1	prediction					1753:1762	the model prediction	1743:1762	the model prediction	1743:1762	The results obtained in this work open the way for the up-scaling of membrane technology on the valorization of dairy effluents by simplifying the operation process and the model prediction and the choice of the membrane.					
37225584	7	60	theme	membrane	1786:1793	arg1	choice					1772:1777	the choice	1768:1777	the choice of the membrane	1768:1793	The results obtained in this work open the way for the up-scaling of membrane technology on the valorization of dairy effluents by simplifying the operation process and the model prediction and the choice of the membrane.					
37225584	7	60	theme	membrane	1786:1793	arg1	process					1731:1737	the operation process	1717:1737	the operation process	1717:1737	The results obtained in this work open the way for the up-scaling of membrane technology on the valorization of dairy effluents by simplifying the operation process and the model prediction and the choice of the membrane.					
37225584	6	61	theme	best	1417:1420	arg1	prediction					1422:1431	the best prediction	1413:1431	the best prediction in the latter case	1413:1450	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	3	62	dep	%	567:567	arg1	40					565:566	40	565:566	40	565:566	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	2	63	from	fermentation	349:360	arg1	recovery					335:342	Lactic acid recovery	323:342	Lactic acid recovery from fermentation broth without precipitation	323:388	Lactic acid recovery from fermentation broth without precipitation has been studied by numerous processes.					
37225584	4	64	from	pH	1003:1004	arg1	charge					850:855	its high negative charge	832:855	its high negative charge	832:855	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	4	64	from	pH	1003:1004	arg1	rejection					930:938	a lactose rejection	920:938	a lactose rejection higher than 98% and a lactic acid rejection lower than 37%	920:997	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	4	64	from	pH	1003:1004	arg1	rejection					898:906	divalent ion rejection	885:906	divalent ion rejection	885:906	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	4	64	from	pH	1003:1004	arg1	point					874:878	low isoelectric point	858:878	low isoelectric point	858:878	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	6	65	theme	±	1531:1531	arg1	m-2					1542:1544	15.06 ± 3.17 L × m-2	1525:1544	15.06 ± 3.17 L × m-2	1525:1544	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	6	66	theme	dissociation	1193:1204	arg1	negligible					1231:1240	negligible	1231:1240	negligible	1231:1240	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	6	66	theme	dissociation	1193:1204	arg1	degree					1206:1211	the dissociation degree	1189:1211	the dissociation degree of lactic acid	1189:1226	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	4	67	dep	film	768:771	arg1	type					803:806	composite nanofiltration (NF) type	773:806	the thin film composite nanofiltration (NF) type	759:806	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	6	68	dep	=	1523:1523	arg1	m-2					1542:1544	15.06 ± 3.17 L × m-2	1525:1544	15.06 ± 3.17 L × m-2	1525:1544	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	4	69	theme	isoelectric	862:872	arg1	point					874:878	low isoelectric point	858:878	low isoelectric point	858:878	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	6	70	theme	=	1523:1523	arg1	h-1					1548:1550	= 15.06 ± 3.17 L × m-2 × h-1	1523:1550	= 15.06 ± 3.17 L × m-2 × h-1	1523:1550	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	4	71	theme	steps	1057:1061	arg1	need					1027:1030	the need	1023:1030	the need of additional separation steps	1023:1061	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	1	72	theme	increasing	108:117	arg1	demand					119:124	The continuously increasing demand	91:124	The continuously increasing demand of lactic acid	91:139	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	6	73	theme	×	1546:1546	arg1	h-1					1548:1550	= 15.06 ± 3.17 L × m-2 × h-1	1523:1550	= 15.06 ± 3.17 L × m-2 × h-1	1523:1550	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	4	74	theme	negative	841:848	arg1	charge					850:855	its high negative charge	832:855	its high negative charge	832:855	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	6	75	theme	h-1	1507:1509	arg1	bar-1					1513:1517	× h-1 × bar-1	1505:1517	× h-1 × bar-1	1505:1517	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	1	76	theme	membrane	179:186	arg1	technology					188:197	membrane technology	179:197	membrane technology	179:197	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	1	77	theme	acid	136:139	arg1	demand					119:124	The continuously increasing demand	91:124	The continuously increasing demand of lactic acid	91:139	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	4	78	theme	additional	1035:1044	arg1	steps					1057:1061	additional separation steps	1035:1061	additional separation steps	1035:1061	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	5	79	theme	feed	1128:1131	arg1	concentration					1133:1145	feed concentration	1128:1145	feed concentration	1128:1145	The experimental lactic acid rejection was evaluated at varying feed concentration, pressure, temperature, and flow rate.					
37225584	1	80	theme	generation	311:320	arg1	chemicals					291:299	chemicals	291:299	chemicals	291:299	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	1	80	theme	generation	311:320	arg1	generation					311:320	waste generation	305:320	waste generation	305:320	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	1	80	theme	generation	311:320	arg1	amounts					280:286	large amounts	274:286	large amounts of chemicals and waste generation	274:320	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	7	81	theme	operation	1721:1729	arg1	process					1731:1737	the operation process	1717:1737	the operation process	1717:1737	The results obtained in this work open the way for the up-scaling of membrane technology on the valorization of dairy effluents by simplifying the operation process and the model prediction and the choice of the membrane.					
37225584	6	82	theme	latter	1440:1445	arg1	case					1447:1450	the latter case	1436:1450	the latter case	1436:1450	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	3	83	dep	permselectivity	543:557	arg1	%					567:567	%	567:567	%	567:567	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	6	84	theme	simulated	1258:1266	arg1	conditions					1268:1277	industrially simulated conditions	1245:1277	industrially simulated conditions	1245:1277	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	6	85	theme	×	1499:1499	arg1	m-2					1501:1503	3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1	1485:1550	m-2	1501:1503	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	6	86	from	prediction	1422:1431	arg1	case					1447:1450	the latter case	1436:1450	the latter case	1436:1450	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	6	87	theme	acid	1223:1226	arg1	negligible					1231:1240	negligible	1231:1240	negligible	1231:1240	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	6	87	theme	acid	1223:1226	arg1	degree					1206:1211	the dissociation degree	1189:1211	the dissociation degree of lactic acid	1189:1226	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	7	88	theme	effluents	1692:1700	arg1	valorization					1670:1681	the valorization	1666:1681	the valorization of dairy effluents	1666:1700	The results obtained in this work open the way for the up-scaling of membrane technology on the valorization of dairy effluents by simplifying the operation process and the model prediction and the choice of the membrane.					
37225584	4	89	theme	divalent	885:892	arg1	rejection					898:906	divalent ion rejection	885:906	divalent ion rejection	885:906	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	6	90	theme	L	1538:1538	arg1	m-2					1542:1544	15.06 ± 3.17 L × m-2	1525:1544	15.06 ± 3.17 L × m-2	1525:1544	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	0	91	from	lactose	39:45	arg1	Modeling					0:7	Modeling	0:7	Modeling of lactic acid rejection from lactose in acidified cheese	0:65	Modeling of lactic acid rejection from lactose in acidified cheese whey by nanofiltration.					
37225584	0	91	from	lactose	39:45	arg1	rejection					24:32	lactic acid rejection	12:32	lactic acid rejection from lactose	12:45	Modeling of lactic acid rejection from lactose in acidified cheese whey by nanofiltration.					
37225584	4	92	theme	higher	940:945	arg1	rejection					930:938	a lactose rejection	920:938	a lactose rejection higher than 98% and a lactic acid rejection lower than 37%	920:997	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	5	93	theme	flow	1175:1178	arg1	rate					1180:1183	flow rate	1175:1183	flow rate	1175:1183	The experimental lactic acid rejection was evaluated at varying feed concentration, pressure, temperature, and flow rate.					
37225584	0	94	theme	acid	19:22	arg1	rejection					24:32	lactic acid rejection	12:32	lactic acid rejection from lactose	12:45	Modeling of lactic acid rejection from lactose in acidified cheese whey by nanofiltration.					
37225584	3	95	theme	commercial	446:455	arg1	membrane					457:464	a commercial membrane	444:464	a commercial membrane	444:464	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	1	96	theme	large	274:278	arg1	chemicals					291:299	chemicals	291:299	chemicals	291:299	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	1	96	theme	large	274:278	arg1	generation					311:320	waste generation	305:320	waste generation	305:320	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	1	96	theme	large	274:278	arg1	amounts					280:286	large amounts	274:286	large amounts of chemicals and waste generation	274:320	The continuously increasing demand of lactic acid opens a window for the integration of membrane technology in the dairy industry, improving the sustainability by avoiding the use of large amounts of chemicals and waste generation.					
37225584	7	97	theme	technology	1652:1661	arg1	up-scaling					1629:1638	the up-scaling	1625:1638	the up-scaling of membrane technology on the valorization of dairy effluents	1625:1700	The results obtained in this work open the way for the up-scaling of membrane technology on the valorization of dairy effluents by simplifying the operation process and the model prediction and the choice of the membrane.					
37225584	4	98	theme	thin	763:766	arg1	film					768:771	the thin film composite nanofiltration (NF) type	759:806	the thin film composite nanofiltration (NF) type	759:806	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	4	99	theme	lactose	922:928	arg1	rejection					930:938	a lactose rejection	920:938	a lactose rejection higher than 98% and a lactic acid rejection lower than 37%	920:997	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	4	100	theme	composite	773:781	arg1	type					803:806	composite nanofiltration (NF) type	773:806	the thin film composite nanofiltration (NF) type	759:806	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	3	101	theme	lactose	476:482	arg1	rejection					484:492	high lactose rejection	471:492	high lactose rejection	471:492	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	6	102	theme	Spiegler-Kedem	1385:1398	arg1	models					1400:1405	Spiegler-Kedem models	1385:1405	Spiegler-Kedem models	1385:1405	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
37225584	3	103	theme	lactic	619:624	arg1	acid					626:629	lactic acid	619:629	lactic acid	619:629	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	4	104	theme	AFC30	741:745	arg1	membrane					747:754	The AFC30 membrane	737:754	The AFC30 membrane of the thin film composite nanofiltration (NF) type	737:806	The AFC30 membrane of the thin film composite nanofiltration (NF) type was selected because of its high negative charge, low isoelectric point, and divalent ion rejection, as well as a lactose rejection higher than 98% and a lactic acid rejection lower than 37%, at pH 3.5, to minimize the need of additional separation steps.					
37225584	3	105	theme	moderate	500:507	arg1	acid					516:519	a moderate lactic acid	498:519	a moderate lactic acid rejection	498:529	In this work, a commercial membrane with high lactose rejection and a moderate lactic acid rejection, enabling a permselectivity up to 40%, is sought to perform the simultaneous removal of lactic acid and lactose separation from the acidified sweet whey from mozzarella cheese production in a single stage.					
37225584	6	106	theme	irreversible	1337:1348	arg1	Kedem-Katchalsky					1364:1379	the irreversible thermodynamic Kedem-Katchalsky	1333:1379	the irreversible thermodynamic Kedem-Katchalsky	1333:1379	As the dissociation degree of lactic acid is negligible in industrially simulated conditions, the performance of this NF membrane was validated by the irreversible thermodynamic Kedem-Katchalsky and Spiegler-Kedem models, with the best prediction in the latter case, with the parameter values: Lp = 3.24 ± 0.87 L × m-2 × h-1 × bar-1 and = 15.06 ± 3.17 L × m-2 × h-1, and σ = 0.45 ± 0.03.					
36906367	10	0	theme	biomedical	1296:1305	arg1	engineering					1307:1317	biomedical engineering	1296:1317	biomedical engineering	1296:1317	Therefore, G/SA-TA/Ca2+ hydrogels are expected to be used in the field of biomedical engineering.					
36906367	1	1	theme	high	169:172	arg1	solution					199:206	high tannic acid (TA) content solution	169:206	high tannic acid (TA) content solution	169:206	The acidity of high tannic acid (TA) content solution can destroy the structure of protein, such as gelatin (G).					
36906367	9	2	theme	Cell	1104:1107	arg1	experiments					1109:1119	Cell experiments	1104:1119	Cell experiments	1104:1119	Cell experiments showed that G/SA-TA/Ca2+ hydrogels possessed good biocompatibility and could promote cell migration.					
36906367	3	3	theme	film	459:462	arg1	strategy					465:472	a "protective film" strategy	445:472	a "protective film" strategy	445:472	Here, the G-based hydrogel system with abundant TA as hydrogen bonds provider was constructed by a "protective film" strategy.					
36906367	9	4	contain	possessed	1156:1164	arg1	hydrogels					1146:1154	G/SA-TA/Ca2+ hydrogels	1133:1154	G/SA-TA/Ca2+ hydrogels	1133:1154	Cell experiments showed that G/SA-TA/Ca2+ hydrogels possessed good biocompatibility and could promote cell migration.					
36906367	9	4	contain	possessed	1156:1164	arg2	biocompatibility					1171:1186	good biocompatibility	1166:1186	good biocompatibility	1166:1186	Cell experiments showed that G/SA-TA/Ca2+ hydrogels possessed good biocompatibility and could promote cell migration.					
36906367	8	5	theme	hemolysis	1087:1095	arg1	retention					1015:1023	good water retention	1004:1023	good water retention	1004:1023	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	8	5	theme	hemolysis	1087:1095	arg1	ratio					1097:1101	low hemolysis ratio	1083:1101	low hemolysis ratio	1083:1101	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	7	6	theme	G/SA	898:901	arg1	hydrogel					903:910	G/SA hydrogel	898:910	G/SA hydrogel	898:910	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	4	7	theme	Ca2+	587:590	arg1	chelation					549:557	the chelation	545:557	the chelation of sodium alginate (SA) and Ca2+	545:590	The protective film around the composite hydrogel was first formed by the chelation of sodium alginate (SA) and Ca2+.					
36906367	8	8	theme	G/SA-TA/Ca2+	971:982	arg1	hydrogels					984:992	G/SA-TA/Ca2+ hydrogels	971:992	G/SA-TA/Ca2+ hydrogels	971:992	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	3	9	theme	protective	448:457	arg1	strategy					465:472	a "protective film" strategy	445:472	a "protective film" strategy	445:472	Here, the G-based hydrogel system with abundant TA as hydrogen bonds provider was constructed by a "protective film" strategy.					
36906367	7	10	theme	0.3 w/v	800:806	arg1	TA					808:809	0.3 w/v TA	800:809	0.3 w/v TA	800:809	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	1	11	theme	gelatin	254:260	arg1	structure					224:232	the structure	220:232	the structure of protein, such as gelatin (G)	220:264	The acidity of high tannic acid (TA) content solution can destroy the structure of protein, such as gelatin (G).					
36906367	5	12	theme	hydrogel	666:673	arg1	system					675:680	the hydrogel system	662:680	the hydrogel system	662:680	Subsequently, abundant TA and Ca2+ were successively introduced into the hydrogel system by immersing method.					
36906367	4	13	theme	alginate	569:576	arg1	chelation					549:557	the chelation	545:557	the chelation of sodium alginate (SA) and Ca2+	545:590	The protective film around the composite hydrogel was first formed by the chelation of sodium alginate (SA) and Ca2+.					
36906367	1	14	theme	acid	181:184	arg1	solution					199:206	high tannic acid (TA) content solution	169:206	high tannic acid (TA) content solution	169:206	The acidity of high tannic acid (TA) content solution can destroy the structure of protein, such as gelatin (G).					
36906367	3	15	theme	G-based	358:364	arg1	system					375:380	the G-based hydrogel system	354:380	the G-based hydrogel system with abundant TA as hydrogen bonds provider	354:424	Here, the G-based hydrogel system with abundant TA as hydrogen bonds provider was constructed by a "protective film" strategy.					
36906367	3	16	theme	hydrogel	366:373	arg1	system					375:380	the G-based hydrogel system	354:380	the G-based hydrogel system with abundant TA as hydrogen bonds provider	354:424	Here, the G-based hydrogel system with abundant TA as hydrogen bonds provider was constructed by a "protective film" strategy.					
36906367	9	17	theme	G/SA-TA/Ca2+	1133:1144	arg1	hydrogels					1146:1154	G/SA-TA/Ca2+ hydrogels	1133:1154	G/SA-TA/Ca2+ hydrogels	1133:1154	Cell experiments showed that G/SA-TA/Ca2+ hydrogels possessed good biocompatibility and could promote cell migration.					
36906367	3	18	with	system	375:380	arg1	provider					417:424	abundant TA as hydrogen bonds provider	387:424	abundant TA as hydrogen bonds provider	387:424	Here, the G-based hydrogel system with abundant TA as hydrogen bonds provider was constructed by a "protective film" strategy.					
36906367	10	19	theme	G/SA-TA/Ca2+	1233:1244	arg1	hydrogels					1246:1254	G/SA-TA/Ca2+ hydrogels	1233:1254	G/SA-TA/Ca2+ hydrogels	1233:1254	Therefore, G/SA-TA/Ca2+ hydrogels are expected to be used in the field of biomedical engineering.					
36906367	2	20	theme	G-based	329:335	arg1	hydrogels					337:345	the G-based hydrogels	325:345	the G-based hydrogels	325:345	This causes a big challenge to introduce abundant TA into the G-based hydrogels.					
36906367	0	21	theme	tannic	9:14	arg1	acid					16:19	Abundant tannic acid	0:19	Abundant tannic acid	0:19	Abundant tannic acid modified gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties.					
36906367	8	22	theme	antibacterial	1054:1066	arg1	properties					1068:1077	antibacterial properties	1054:1077	antibacterial properties	1054:1077	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	8	22	theme	antibacterial	1054:1066	arg1	retention					1015:1023	good water retention	1004:1023	good water retention	1004:1023	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	2	23	theme	big	281:283	arg1	challenge					285:293	a big challenge	279:293	a big challenge to introduce abundant TA into the G-based hydrogels	279:345	This causes a big challenge to introduce abundant TA into the G-based hydrogels.					
36906367	0	24	theme	Abundant	0:7	arg1	acid					16:19	Abundant tannic acid	0:19	Abundant tannic acid	0:19	Abundant tannic acid modified gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties.					
36906367	6	25	theme	designed	760:767	arg1	hydrogel					769:776	the designed hydrogel	756:776	the designed hydrogel	756:776	This strategy effectively protected the structure of the designed hydrogel.					
36906367	9	26	theme	cell	1206:1209	arg1	migration					1211:1219	cell migration	1206:1219	cell migration	1206:1219	Cell experiments showed that G/SA-TA/Ca2+ hydrogels possessed good biocompatibility and could promote cell migration.					
36906367	3	27	theme	abundant	387:394	arg1	provider					417:424	abundant TA as hydrogen bonds provider	387:424	abundant TA as hydrogen bonds provider	387:424	Here, the G-based hydrogel system with abundant TA as hydrogen bonds provider was constructed by a "protective film" strategy.					
36906367	7	28	theme	0.06 w/v	815:822	arg1	solutions					829:837	0.06 w/v Ca2+ solutions	815:837	0.06 w/v Ca2+ solutions	815:837	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	8	29	theme	good	1004:1007	arg1	properties					1068:1077	antibacterial properties	1054:1077	antibacterial properties	1054:1077	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	8	29	theme	good	1004:1007	arg1	retention					1015:1023	good water retention	1004:1023	good water retention	1004:1023	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	8	29	theme	good	1004:1007	arg1	ratio					1097:1101	low hemolysis ratio	1083:1101	low hemolysis ratio	1083:1101	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	9	30	theme	good	1166:1169	arg1	biocompatibility					1171:1186	good biocompatibility	1166:1186	good biocompatibility	1166:1186	Cell experiments showed that G/SA-TA/Ca2+ hydrogels possessed good biocompatibility and could promote cell migration.					
36906367	11	31	theme	other	1412:1416	arg1	hydrogels					1432:1440	other protein-based hydrogels	1412:1440	other protein-based hydrogels	1412:1440	The strategy proposed in this work also provides a new idea for improving the properties of other protein-based hydrogels.					
36906367	1	32	theme	content	191:197	arg1	solution					199:206	high tannic acid (TA) content solution	169:206	high tannic acid (TA) content solution	169:206	The acidity of high tannic acid (TA) content solution can destroy the structure of protein, such as gelatin (G).					
36906367	0	33	theme	antibacterial	128:140	arg1	properties					142:151	antibacterial properties	128:151	antibacterial properties	128:151	Abundant tannic acid modified gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties.					
36906367	1	34	theme	tannic	174:179	arg1	TA					187:188	TA	187:188	TA	187:188	The acidity of high tannic acid (TA) content solution can destroy the structure of protein, such as gelatin (G).					
36906367	1	34	theme	tannic	174:179	arg1	acid					181:184	tannic acid	174:184	high tannic acid (TA) content solution	169:206	The acidity of high tannic acid (TA) content solution can destroy the structure of protein, such as gelatin (G).					
36906367	1	35	theme	solution	199:206	arg1	acidity					158:164	The acidity	154:164	The acidity of high tannic acid (TA) content solution	154:206	The acidity of high tannic acid (TA) content solution can destroy the structure of protein, such as gelatin (G).					
36906367	0	36	theme	alginate	45:52	arg1	hydrogels					67:75	gelatin/sodium alginate biocomposite hydrogels	30:75	gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties	30:151	Abundant tannic acid modified gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties.					
36906367	4	37	theme	composite	506:514	arg1	hydrogel					516:523	the composite hydrogel	502:523	the composite hydrogel	502:523	The protective film around the composite hydrogel was first formed by the chelation of sodium alginate (SA) and Ca2+.					
36906367	3	38	theme	hydrogen	402:409	arg1	bonds					411:415	hydrogen bonds	402:415	hydrogen bonds	402:415	Here, the G-based hydrogel system with abundant TA as hydrogen bonds provider was constructed by a "protective film" strategy.					
36906367	0	39	theme	gelatin/sodium	30:43	arg1	hydrogels					67:75	gelatin/sodium alginate biocomposite hydrogels	30:75	gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties	30:151	Abundant tannic acid modified gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties.					
36906367	8	40	theme	water	1009:1013	arg1	properties					1068:1077	antibacterial properties	1054:1077	antibacterial properties	1054:1077	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	8	40	theme	water	1009:1013	arg1	retention					1015:1023	good water retention	1004:1023	good water retention	1004:1023	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	8	40	theme	water	1009:1013	arg1	ratio					1097:1101	low hemolysis ratio	1083:1101	low hemolysis ratio	1083:1101	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	8	41	dep	anti-freezing	1026:1038	arg1	antioxidant					1041:1051	antioxidant	1041:1051	antioxidant	1041:1051	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	8	42	theme	anti-freezing	1026:1038	arg1	properties					1068:1077	antibacterial properties	1054:1077	antibacterial properties	1054:1077	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	8	42	theme	anti-freezing	1026:1038	arg1	retention					1015:1023	good water retention	1004:1023	good water retention	1004:1023	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	8	42	theme	anti-freezing	1026:1038	arg1	ratio					1097:1101	low hemolysis ratio	1083:1101	low hemolysis ratio	1083:1101	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	3	43	theme	TA	396:397	arg1	provider					417:424	abundant TA as hydrogen bonds provider	387:424	abundant TA as hydrogen bonds provider	387:424	Here, the G-based hydrogel system with abundant TA as hydrogen bonds provider was constructed by a "protective film" strategy.					
36906367	2	44	theme	abundant	308:315	arg1	TA					317:318	abundant TA	308:318	abundant TA	308:318	This causes a big challenge to introduce abundant TA into the G-based hydrogels.					
36906367	0	45	theme	biocomposite	54:65	arg1	hydrogels					67:75	gelatin/sodium alginate biocomposite hydrogels	30:75	gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties	30:151	Abundant tannic acid modified gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties.					
36906367	10	46	used	used	1275:1278	arg2	hydrogels					1246:1254	G/SA-TA/Ca2+ hydrogels	1233:1254	G/SA-TA/Ca2+ hydrogels	1233:1254	Therefore, G/SA-TA/Ca2+ hydrogels are expected to be used in the field of biomedical engineering.					
36906367	5	47	theme	abundant	607:614	arg1	TA					616:617	abundant TA	607:617	abundant TA	607:617	Subsequently, abundant TA and Ca2+ were successively introduced into the hydrogel system by immersing method.					
36906367	7	48	with	treatment	785:793	arg1	solutions					829:837	0.06 w/v Ca2+ solutions	815:837	0.06 w/v Ca2+ solutions	815:837	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	7	48	with	treatment	785:793	arg1	TA					808:809	0.3 w/v TA	800:809	0.3 w/v TA	800:809	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	7	49	theme	tensile	844:850	arg1	modulus					852:858	the tensile modulus	840:858	the tensile modulus	840:858	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	4	50	theme	protective	479:488	arg1	film					490:493	The protective film	475:493	The protective film around the composite hydrogel	475:523	The protective film around the composite hydrogel was first formed by the chelation of sodium alginate (SA) and Ca2+.					
36906367	8	51	theme	low	1083:1085	arg1	retention					1015:1023	good water retention	1004:1023	good water retention	1004:1023	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	8	51	theme	low	1083:1085	arg1	ratio					1097:1101	low hemolysis ratio	1083:1101	low hemolysis ratio	1083:1101	Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio.					
36906367	10	52	theme	engineering	1307:1317	arg1	field					1287:1291	the field	1283:1291	the field of biomedical engineering	1283:1317	Therefore, G/SA-TA/Ca2+ hydrogels are expected to be used in the field of biomedical engineering.					
36906367	6	53	theme	hydrogel	769:776	arg1	structure					743:751	the structure	739:751	the structure of the designed hydrogel	739:776	This strategy effectively protected the structure of the designed hydrogel.					
36906367	0	54	with	hydrogels	67:75	arg1	toughness					87:95	high toughness	82:95	high toughness	82:95	Abundant tannic acid modified gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties.					
36906367	0	54	with	hydrogels	67:75	arg1	antioxidant					112:122	antioxidant	112:122	antioxidant	112:122	Abundant tannic acid modified gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties.					
36906367	0	54	with	hydrogels	67:75	arg1	properties					142:151	antibacterial properties	128:151	antibacterial properties	128:151	Abundant tannic acid modified gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties.					
36906367	0	54	with	hydrogels	67:75	arg1	antifreezing					98:109	antifreezing	98:109	antifreezing	98:109	Abundant tannic acid modified gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties.					
36906367	7	55	from	break	875:879	arg1	toughness					885:893	toughness	885:893	toughness of G/SA hydrogel	885:910	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	7	55	from	break	875:879	arg1	elongation					861:870	elongation	861:870	elongation at break	861:879	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	7	55	from	break	875:879	arg1	modulus					852:858	the tensile modulus	840:858	the tensile modulus	840:858	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	11	56	theme	protein-based	1418:1430	arg1	hydrogels					1432:1440	other protein-based hydrogels	1412:1440	other protein-based hydrogels	1412:1440	The strategy proposed in this work also provides a new idea for improving the properties of other protein-based hydrogels.					
36906367	11	57	theme	new	1371:1373	arg1	idea					1375:1378	a new idea	1369:1378	a new idea for improving the properties of other protein-based hydrogels	1369:1440	The strategy proposed in this work also provides a new idea for improving the properties of other protein-based hydrogels.					
36906367	0	58	theme	high	82:85	arg1	toughness					87:95	high toughness	82:95	high toughness	82:95	Abundant tannic acid modified gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties.					
36906367	1	59	dep	such	246:249	arg1	as					251:252	as	251:252	as	251:252	The acidity of high tannic acid (TA) content solution can destroy the structure of protein, such as gelatin (G).					
36906367	3	60	theme	"	463:463	arg1	strategy					465:472	a "protective film" strategy	445:472	a "protective film" strategy	445:472	Here, the G-based hydrogel system with abundant TA as hydrogen bonds provider was constructed by a "protective film" strategy.					
36906367	11	61	theme	hydrogels	1432:1440	arg1	properties					1398:1407	the properties	1394:1407	the properties of other protein-based hydrogels	1394:1440	The strategy proposed in this work also provides a new idea for improving the properties of other protein-based hydrogels.					
36906367	4	62	theme	sodium	562:567	arg1	SA					579:580	SA	579:580	SA	579:580	The protective film around the composite hydrogel was first formed by the chelation of sodium alginate (SA) and Ca2+.					
36906367	4	62	theme	sodium	562:567	arg1	alginate					569:576	sodium alginate	562:576	sodium alginate (SA)	562:581	The protective film around the composite hydrogel was first formed by the chelation of sodium alginate (SA) and Ca2+.					
36906367	7	63	theme	hydrogel	903:910	arg1	toughness					885:893	toughness	885:893	toughness of G/SA hydrogel	885:910	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	7	63	theme	hydrogel	903:910	arg1	elongation					861:870	elongation	861:870	elongation at break	861:879	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	7	63	theme	hydrogel	903:910	arg1	modulus					852:858	the tensile modulus	840:858	the tensile modulus	840:858	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	7	64	theme	Ca2+	824:827	arg1	solutions					829:837	0.06 w/v Ca2+ solutions	815:837	0.06 w/v Ca2+ solutions	815:837	After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively.					
36906367	1	65	theme	protein	237:243	arg1	structure					224:232	the structure	220:232	the structure of protein, such as gelatin (G)	220:264	The acidity of high tannic acid (TA) content solution can destroy the structure of protein, such as gelatin (G).					
36086796	7	0	theme	matrices	1149:1156	arg1	influence					1136:1144	the influence	1132:1144	the influence of matrices	1132:1156	Due to the complex composition of luncheon meat, the real samples were rinsed with deionized water to reduce the influence of matrices.					
36086796	8	1	dep	amines	1273:1278	arg1	content					1280:1286	content	1280:1286	content	1280:1286	It was found that both storage temperature and protein content of the luncheon meat samples affected the biogenic amines content during storage.					
36086796	9	2	theme	study	1324:1328	arg1	instructive					1334:1344	instructive	1334:1344	instructive	1334:1344	The results of this study are instructive for the storage of high-protein meat products.					
36086796	9	2	theme	study	1324:1328	arg1	results					1308:1314	The results	1304:1314	The results of this study	1304:1328	The results of this study are instructive for the storage of high-protein meat products.					
36086796	8	3	theme	biogenic	1264:1271	arg1	amines					1273:1278	the biogenic amines	1260:1278	the biogenic amines content during storage	1260:1301	It was found that both storage temperature and protein content of the luncheon meat samples affected the biogenic amines content during storage.					
36086796	6	4	theme	meat	1009:1012	arg1	samples					1014:1020	commercial luncheon meat samples	989:1020	commercial luncheon meat samples	989:1020	To verify the applicability, this method was carried out to analyze biogenic amines in commercial luncheon meat samples.					
36086796	1	5	theme	accurate	137:144	arg1	electrophoresis					167:181	a simple, accurate, and rapid capillary electrophoresis	127:181	a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method	127:227	In this work, a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method was developed to simultaneous separate 10 biogenic amines.					
36086796	8	6	theme	samples	1243:1249	arg1	temperature					1190:1200	storage temperature	1182:1200	storage temperature	1182:1200	It was found that both storage temperature and protein content of the luncheon meat samples affected the biogenic amines content during storage.					
36086796	8	6	theme	samples	1243:1249	arg1	content					1214:1220	protein content	1206:1220	protein content of the luncheon meat samples	1206:1249	It was found that both storage temperature and protein content of the luncheon meat samples affected the biogenic amines content during storage.					
36086796	0	7	theme	ultraviolet	90:100	arg1	detection					102:110	indirect ultraviolet detection	81:110	indirect ultraviolet detection	81:110	Study the key biogenic amines in luncheon meat by capillary electrophoresis with indirect ultraviolet detection.					
36086796	7	8	theme	luncheon	1057:1064	arg1	meat					1066:1069	luncheon meat	1057:1069	luncheon meat	1057:1069	Due to the complex composition of luncheon meat, the real samples were rinsed with deionized water to reduce the influence of matrices.					
36086796	0	9	with	electrophoresis	60:74	arg1	detection					102:110	indirect ultraviolet detection	81:110	indirect ultraviolet detection	81:110	Study the key biogenic amines in luncheon meat by capillary electrophoresis with indirect ultraviolet detection.					
36086796	3	10	theme	diamine	574:580	arg1	acid					594:597	0.5 mmol/L ethylene diamine tetraacetic acid	554:597	0.5 mmol/L ethylene diamine tetraacetic acid	554:597	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	4	11	theme	detection	733:741	arg1	limits					723:728	limits	723:728	limits of detection	723:741	The total analysis time of this method was less than 11 min with limits of detection in the range of 0.14-1.98 mg/L.					
36086796	1	12	theme	simultaneous	246:257	arg1	amines					280:285	simultaneous separate 10 biogenic amines	246:285	simultaneous separate 10 biogenic amines	246:285	In this work, a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method was developed to simultaneous separate 10 biogenic amines.					
36086796	0	13	theme	indirect	81:88	arg1	detection					102:110	indirect ultraviolet detection	81:110	indirect ultraviolet detection	81:110	Study the key biogenic amines in luncheon meat by capillary electrophoresis with indirect ultraviolet detection.					
36086796	7	14	theme	meat	1066:1069	arg1	composition					1042:1052	the complex composition	1030:1052	the complex composition of luncheon meat	1030:1069	Due to the complex composition of luncheon meat, the real samples were rinsed with deionized water to reduce the influence of matrices.					
36086796	3	15	theme	tetraacetic	582:592	arg1	acid					594:597	0.5 mmol/L ethylene diamine tetraacetic acid	554:597	0.5 mmol/L ethylene diamine tetraacetic acid	554:597	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	3	16	from	pH	622:623	arg1	imidazole					516:524	20.0 mmol/L imidazole	504:524	20.0 mmol/L imidazole	504:524	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	3	16	from	pH	622:623	arg1	β-cyclodextrin					538:551	8.0 mmol/L β-cyclodextrin	527:551	8.0 mmol/L β-cyclodextrin	527:551	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	3	16	from	pH	622:623	arg1	methanol					609:616	6.0% methanol	604:616	6.0% methanol (at pH 4.50 adjusted with acetic acid)	604:655	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	3	16	from	pH	622:623	arg1	acid					594:597	0.5 mmol/L ethylene diamine tetraacetic acid	554:597	0.5 mmol/L ethylene diamine tetraacetic acid	554:597	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	1	17	theme	separate	259:266	arg1	amines					280:285	simultaneous separate 10 biogenic amines	246:285	simultaneous separate 10 biogenic amines	246:285	In this work, a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method was developed to simultaneous separate 10 biogenic amines.					
36086796	4	18	theme	method	690:695	arg1	less					701:704	less	701:704	less	701:704	The total analysis time of this method was less than 11 min with limits of detection in the range of 0.14-1.98 mg/L.					
36086796	4	18	theme	method	690:695	arg1	time					677:680	The total analysis time	658:680	The total analysis time of this method	658:695	The total analysis time of this method was less than 11 min with limits of detection in the range of 0.14-1.98 mg/L.					
36086796	3	19	theme	20.0 mmol/L	504:514	arg1	imidazole					516:524	20.0 mmol/L imidazole	504:524	20.0 mmol/L imidazole	504:524	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	7	20	theme	deionized	1106:1114	arg1	water					1116:1120	deionized water	1106:1120	deionized water	1106:1120	Due to the complex composition of luncheon meat, the real samples were rinsed with deionized water to reduce the influence of matrices.					
36086796	3	21	theme	0.5 mmol/L	554:563	arg1	acid					594:597	0.5 mmol/L ethylene diamine tetraacetic acid	554:597	0.5 mmol/L ethylene diamine tetraacetic acid	554:597	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	8	22	theme	storage	1182:1188	arg1	temperature					1190:1200	storage temperature	1182:1200	storage temperature	1182:1200	It was found that both storage temperature and protein content of the luncheon meat samples affected the biogenic amines content during storage.					
36086796	4	23	theme	total	662:666	arg1	less					701:704	less	701:704	less	701:704	The total analysis time of this method was less than 11 min with limits of detection in the range of 0.14-1.98 mg/L.					
36086796	4	23	theme	total	662:666	arg1	time					677:680	The total analysis time	658:680	The total analysis time of this method	658:695	The total analysis time of this method was less than 11 min with limits of detection in the range of 0.14-1.98 mg/L.					
36086796	5	24	theme	relative	788:795	arg1	deviation					806:814	The interday relative standard deviation	775:814	The interday relative standard deviation of migration time and peak area	775:846	The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n = 6), respectively.					
36086796	5	24	theme	relative	788:795	arg1	%					867:867	less than 1.36%	853:867	less than 1.36%	853:867	The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n = 6), respectively.					
36086796	3	25	theme	ethylene	565:572	arg1	acid					594:597	0.5 mmol/L ethylene diamine tetraacetic acid	554:597	0.5 mmol/L ethylene diamine tetraacetic acid	554:597	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	2	26	theme	tryptamine	389:398	arg1	separation					375:384	the separation	371:384	the separation of tryptamine and tyramine, spermidine and spermine	371:436	It was found that β-cyclodextrin and ethylene diamine tetraacetic acid facilitated the separation of tryptamine and tyramine, spermidine and spermine, respectively.					
36086796	5	27	theme	standard	797:804	arg1	deviation					806:814	The interday relative standard deviation	775:814	The interday relative standard deviation of migration time and peak area	775:846	The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n = 6), respectively.					
36086796	5	27	theme	standard	797:804	arg1	%					867:867	less than 1.36%	853:867	less than 1.36%	853:867	The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n = 6), respectively.					
36086796	0	28	from	amines	23:28	arg1	meat					42:45	luncheon meat	33:45	luncheon meat	33:45	Study the key biogenic amines in luncheon meat by capillary electrophoresis with indirect ultraviolet detection.					
36086796	1	29	theme	rapid	151:155	arg1	electrophoresis					167:181	a simple, accurate, and rapid capillary electrophoresis	127:181	a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method	127:227	In this work, a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method was developed to simultaneous separate 10 biogenic amines.					
36086796	7	30	theme	complex	1034:1040	arg1	composition					1042:1052	the complex composition	1030:1052	the complex composition of luncheon meat	1030:1069	Due to the complex composition of luncheon meat, the real samples were rinsed with deionized water to reduce the influence of matrices.					
36086796	3	31	theme	6.0	604:606	arg1	%					607:607	%	607:607	%	607:607	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	1	32	theme	capillary	157:165	arg1	electrophoresis					167:181	a simple, accurate, and rapid capillary electrophoresis	127:181	a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method	127:227	In this work, a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method was developed to simultaneous separate 10 biogenic amines.					
36086796	0	33	theme	biogenic	14:21	arg1	amines					23:28	the key biogenic amines	6:28	the key biogenic amines in luncheon meat	6:45	Study the key biogenic amines in luncheon meat by capillary electrophoresis with indirect ultraviolet detection.					
36086796	1	34	theme	biogenic	271:278	arg1	amines					280:285	simultaneous separate 10 biogenic amines	246:285	simultaneous separate 10 biogenic amines	246:285	In this work, a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method was developed to simultaneous separate 10 biogenic amines.					
36086796	3	35	theme	background	467:476	arg1	electrolytes					478:489	The optimized background electrolytes	453:489	The optimized background electrolytes	453:489	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	0	36	theme	key	10:12	arg1	amines					23:28	the key biogenic amines	6:28	the key biogenic amines in luncheon meat	6:45	Study the key biogenic amines in luncheon meat by capillary electrophoresis with indirect ultraviolet detection.					
36086796	4	37	theme	0.14-1.98 mg/L	759:772	arg1	range					750:754	the range	746:754	the range of 0.14-1.98 mg/L	746:772	The total analysis time of this method was less than 11 min with limits of detection in the range of 0.14-1.98 mg/L.					
36086796	2	38	theme	tetraacetic	342:352	arg1	acid					354:357	ethylene diamine tetraacetic acid	325:357	ethylene diamine tetraacetic acid	325:357	It was found that β-cyclodextrin and ethylene diamine tetraacetic acid facilitated the separation of tryptamine and tyramine, spermidine and spermine, respectively.					
36086796	6	39	theme	luncheon	1000:1007	arg1	samples					1014:1020	commercial luncheon meat samples	989:1020	commercial luncheon meat samples	989:1020	To verify the applicability, this method was carried out to analyze biogenic amines in commercial luncheon meat samples.					
36086796	1	40	with	electrophoresis	167:181	arg1	method					222:227	an indirect ultraviolet detection method	188:227	an indirect ultraviolet detection method	188:227	In this work, a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method was developed to simultaneous separate 10 biogenic amines.					
36086796	6	41	theme	commercial	989:998	arg1	samples					1014:1020	commercial luncheon meat samples	989:1020	commercial luncheon meat samples	989:1020	To verify the applicability, this method was carried out to analyze biogenic amines in commercial luncheon meat samples.					
36086796	4	42	theme	analysis	668:675	arg1	less					701:704	less	701:704	less	701:704	The total analysis time of this method was less than 11 min with limits of detection in the range of 0.14-1.98 mg/L.					
36086796	4	42	theme	analysis	668:675	arg1	time					677:680	The total analysis time	658:680	The total analysis time of this method	658:695	The total analysis time of this method was less than 11 min with limits of detection in the range of 0.14-1.98 mg/L.					
36086796	5	43	theme	peak	838:841	arg1	area					843:846	peak area	838:846	peak area	838:846	The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n = 6), respectively.					
36086796	9	44	theme	high-protein	1365:1376	arg1	products					1383:1390	high-protein meat products	1365:1390	high-protein meat products	1365:1390	The results of this study are instructive for the storage of high-protein meat products.					
36086796	3	45	theme	acetic	644:649	arg1	acid					651:654	acetic acid	644:654	acetic acid	644:654	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	5	46	theme	area	843:846	arg1	deviation					806:814	The interday relative standard deviation	775:814	The interday relative standard deviation of migration time and peak area	775:846	The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n = 6), respectively.					
36086796	5	46	theme	area	843:846	arg1	%					867:867	less than 1.36%	853:867	less than 1.36%	853:867	The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n = 6), respectively.					
36086796	1	47	theme	indirect	191:198	arg1	method					222:227	an indirect ultraviolet detection method	188:227	an indirect ultraviolet detection method	188:227	In this work, a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method was developed to simultaneous separate 10 biogenic amines.					
36086796	5	48	theme	interday	779:786	arg1	deviation					806:814	The interday relative standard deviation	775:814	The interday relative standard deviation of migration time and peak area	775:846	The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n = 6), respectively.					
36086796	5	48	theme	interday	779:786	arg1	%					867:867	less than 1.36%	853:867	less than 1.36%	853:867	The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n = 6), respectively.					
36086796	3	49	theme	%	607:607	arg1	methanol					609:616	6.0% methanol	604:616	6.0% methanol (at pH 4.50 adjusted with acetic acid)	604:655	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	3	50	theme	optimized	457:465	arg1	electrolytes					478:489	The optimized background electrolytes	453:489	The optimized background electrolytes	453:489	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	2	51	theme	diamine	334:340	arg1	acid					354:357	ethylene diamine tetraacetic acid	325:357	ethylene diamine tetraacetic acid	325:357	It was found that β-cyclodextrin and ethylene diamine tetraacetic acid facilitated the separation of tryptamine and tyramine, spermidine and spermine, respectively.					
36086796	0	52	theme	luncheon	33:40	arg1	meat					42:45	luncheon meat	33:45	luncheon meat	33:45	Study the key biogenic amines in luncheon meat by capillary electrophoresis with indirect ultraviolet detection.					
36086796	3	53	theme	8.0 mmol/L	527:536	arg1	β-cyclodextrin					538:551	8.0 mmol/L β-cyclodextrin	527:551	8.0 mmol/L β-cyclodextrin	527:551	The optimized background electrolytes consisted of 20.0 mmol/L imidazole, 8.0 mmol/L β-cyclodextrin, 0.5 mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid).					
36086796	2	54	theme	ethylene	325:332	arg1	acid					354:357	ethylene diamine tetraacetic acid	325:357	ethylene diamine tetraacetic acid	325:357	It was found that β-cyclodextrin and ethylene diamine tetraacetic acid facilitated the separation of tryptamine and tyramine, spermidine and spermine, respectively.					
36086796	6	55	theme	biogenic	970:977	arg1	amines					979:984	biogenic amines	970:984	biogenic amines	970:984	To verify the applicability, this method was carried out to analyze biogenic amines in commercial luncheon meat samples.					
36086796	8	56	theme	meat	1238:1241	arg1	samples					1243:1249	the luncheon meat samples	1225:1249	the luncheon meat samples	1225:1249	It was found that both storage temperature and protein content of the luncheon meat samples affected the biogenic amines content during storage.					
36086796	1	57	theme	ultraviolet	200:210	arg1	method					222:227	an indirect ultraviolet detection method	188:227	an indirect ultraviolet detection method	188:227	In this work, a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method was developed to simultaneous separate 10 biogenic amines.					
36086796	8	58	theme	luncheon	1229:1236	arg1	samples					1243:1249	the luncheon meat samples	1225:1249	the luncheon meat samples	1225:1249	It was found that both storage temperature and protein content of the luncheon meat samples affected the biogenic amines content during storage.					
36086796	1	59	theme	detection	212:220	arg1	method					222:227	an indirect ultraviolet detection method	188:227	an indirect ultraviolet detection method	188:227	In this work, a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method was developed to simultaneous separate 10 biogenic amines.					
36086796	0	60	theme	capillary	50:58	arg1	electrophoresis					60:74	capillary electrophoresis	50:74	capillary electrophoresis with indirect ultraviolet detection	50:110	Study the key biogenic amines in luncheon meat by capillary electrophoresis with indirect ultraviolet detection.					
36086796	2	61	theme	tyramine	404:411	arg1	separation					375:384	the separation	371:384	the separation of tryptamine and tyramine, spermidine and spermine	371:436	It was found that β-cyclodextrin and ethylene diamine tetraacetic acid facilitated the separation of tryptamine and tyramine, spermidine and spermine, respectively.					
36086796	8	62	theme	protein	1206:1212	arg1	content					1214:1220	protein content	1206:1220	protein content of the luncheon meat samples	1206:1249	It was found that both storage temperature and protein content of the luncheon meat samples affected the biogenic amines content during storage.					
36086796	5	63	theme	time	829:832	arg1	deviation					806:814	The interday relative standard deviation	775:814	The interday relative standard deviation of migration time and peak area	775:846	The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n = 6), respectively.					
36086796	5	63	theme	time	829:832	arg1	%					867:867	less than 1.36%	853:867	less than 1.36%	853:867	The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n = 6), respectively.					
36086796	9	64	theme	meat	1378:1381	arg1	products					1383:1390	high-protein meat products	1365:1390	high-protein meat products	1365:1390	The results of this study are instructive for the storage of high-protein meat products.					
36086796	7	65	theme	real	1076:1079	arg1	samples					1081:1087	the real samples	1072:1087	the real samples	1072:1087	Due to the complex composition of luncheon meat, the real samples were rinsed with deionized water to reduce the influence of matrices.					
36086796	9	66	theme	products	1383:1390	arg1	storage					1354:1360	the storage	1350:1360	the storage of high-protein meat products	1350:1390	The results of this study are instructive for the storage of high-protein meat products.					
36086796	5	67	theme	migration	819:827	arg1	time					829:832	migration time	819:832	migration time	819:832	The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n = 6), respectively.					
36086796	1	68	theme	simple	129:134	arg1	electrophoresis					167:181	a simple, accurate, and rapid capillary electrophoresis	127:181	a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method	127:227	In this work, a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method was developed to simultaneous separate 10 biogenic amines.					
35202469	3	0	theme	total	887:891	arg1	nitrogen					908:915	total volatile basic nitrogen	887:915	total volatile basic nitrogen	887:915	The results showed that the treatment of CS-g-GA and CS-g-PA could retard the increase of pH, total volatile basic nitrogen, and the K value.					
35202469	9	1	dep	analysis	1737:1744	arg1	basis					1724:1728	basis	1724:1728	basis	1724:1728	On the basis of the analysis of the microorganism and physicochemical quality, compared with the CK group, CS-g-GA and CS-g-PA can maintain the good quality of sea bass fillets and prolong the shelf life for another 12 days.					
35202469	9	1	dep	analysis	1737:1744	arg1	the					1720:1722	the	1720:1722	the	1720:1722	On the basis of the analysis of the microorganism and physicochemical quality, compared with the CK group, CS-g-GA and CS-g-PA can maintain the good quality of sea bass fillets and prolong the shelf life for another 12 days.					
35202469	2	2	theme	CS	740:741	arg1	derivatives					743:753	CS derivatives	740:753	CS derivatives	740:753	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	4	3	theme	bacteria	1031:1038	arg1	production					977:986	production	977:986	production of biogenic amines	977:1005	The degradation of ATP-related compounds, production of biogenic amines, and growth of spoilage bacteria were inhibited by CS-g-GA and CS-g-PA.					
35202469	4	3	theme	bacteria	1031:1038	arg1	degradation					939:949	The degradation	935:949	The degradation of ATP-related compounds	935:974	The degradation of ATP-related compounds, production of biogenic amines, and growth of spoilage bacteria were inhibited by CS-g-GA and CS-g-PA.					
35202469	4	3	theme	bacteria	1031:1038	arg1	growth					1012:1017	growth	1012:1017	growth of spoilage bacteria	1012:1038	The degradation of ATP-related compounds, production of biogenic amines, and growth of spoilage bacteria were inhibited by CS-g-GA and CS-g-PA.					
35202469	2	4	theme	%	482:482	arg1	copolymer					517:525	1% CS-grafted gallic acid (CS-g-GA) copolymer	481:525	1% CS-grafted gallic acid (CS-g-GA) copolymer	481:525	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	4	5	theme	amines	1000:1005	arg1	production					977:986	production	977:986	production of biogenic amines	977:1005	The degradation of ATP-related compounds, production of biogenic amines, and growth of spoilage bacteria were inhibited by CS-g-GA and CS-g-PA.					
35202469	4	5	theme	amines	1000:1005	arg1	degradation					939:949	The degradation	935:949	The degradation of ATP-related compounds	935:974	The degradation of ATP-related compounds, production of biogenic amines, and growth of spoilage bacteria were inhibited by CS-g-GA and CS-g-PA.					
35202469	4	5	theme	amines	1000:1005	arg1	growth					1012:1017	growth	1012:1017	growth of spoilage bacteria	1012:1038	The degradation of ATP-related compounds, production of biogenic amines, and growth of spoilage bacteria were inhibited by CS-g-GA and CS-g-PA.					
35202469	2	6	theme	microbiological	602:616	arg1	indicators					638:647	the microbiological and physicochemical indicators	598:647	the microbiological and physicochemical indicators	598:647	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	1	7	theme	bass	372:375	arg1	quality					314:320	quality	314:320	quality	314:320	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	1	7	theme	bass	372:375	arg1	composition					337:347	microbiota composition	326:347	microbiota composition	326:347	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	4	8	theme	compounds	966:974	arg1	production					977:986	production	977:986	production of biogenic amines	977:1005	The degradation of ATP-related compounds, production of biogenic amines, and growth of spoilage bacteria were inhibited by CS-g-GA and CS-g-PA.					
35202469	4	8	theme	compounds	966:974	arg1	degradation					939:949	The degradation	935:949	The degradation of ATP-related compounds	935:974	The degradation of ATP-related compounds, production of biogenic amines, and growth of spoilage bacteria were inhibited by CS-g-GA and CS-g-PA.					
35202469	4	8	theme	compounds	966:974	arg1	growth					1012:1017	growth	1012:1017	growth of spoilage bacteria	1012:1038	The degradation of ATP-related compounds, production of biogenic amines, and growth of spoilage bacteria were inhibited by CS-g-GA and CS-g-PA.					
35202469	2	9	theme	RNA	683:685	arg1	sequencing					703:712	RNA high-throughput sequencing	683:712	RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition	683:790	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	1	10	theme	Lateolabrax	378:388	arg1	bass					372:375	vacuum-packaged sea bass	352:375	vacuum-packaged sea bass (Lateolabrax japonicus)	352:399	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	1	10	theme	Lateolabrax	378:388	arg1	japonicus					390:398	Lateolabrax japonicus	378:398	Lateolabrax japonicus	378:398	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	0	11	theme	Chilled	155:161	arg1	Storage					163:169	Chilled Storage	155:169	Chilled Storage	155:169	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	6	12	from	diversity	1319:1327	arg1	groups					1361:1366	all groups	1357:1366	all groups	1357:1366	According to the results of high-throughput sequencing, the diversity of microbial composition in all groups was decreased significantly during chilled storage, especially in the CK group.					
35202469	2	13	theme	gallic	495:500	arg1	CS-g-GA					508:514	CS-g-GA	508:514	CS-g-GA	508:514	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	2	13	theme	gallic	495:500	arg1	acid					502:505	CS-grafted gallic acid	484:505	1% CS-grafted gallic acid (CS-g-GA) copolymer	481:525	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	9	14	theme	bass	1881:1884	arg1	fillets					1886:1892	sea bass fillets	1877:1892	sea bass fillets	1877:1892	On the basis of the analysis of the microorganism and physicochemical quality, compared with the CK group, CS-g-GA and CS-g-PA can maintain the good quality of sea bass fillets and prolong the shelf life for another 12 days.					
35202469	7	15	theme	storage	1520:1526	arg1	period					1510:1515	the middle period	1499:1515	the middle period of storage	1499:1526	The predominant microorganism was Acinetobacter in the middle period of storage, while Pseudomonas and Shewanella became predominant at the end of storage.					
35202469	4	16	theme	ATP-related	954:964	arg1	compounds					966:974	ATP-related compounds	954:974	ATP-related compounds	954:974	The degradation of ATP-related compounds, production of biogenic amines, and growth of spoilage bacteria were inhibited by CS-g-GA and CS-g-PA.					
35202469	5	17	theme	thiobarbituric	1182:1195	arg1	substances					1211:1220	thiobarbituric acid reactive substances	1182:1220	thiobarbituric acid reactive substances	1182:1220	Moreover, CS-g-GA and CS-g-PA performed better in the inhibition of lipid oxidation by the analysis of thiobarbituric acid reactive substances and relative fluorescence intensity.					
35202469	6	18	theme	sequencing	1303:1312	arg1	results					1276:1282	the results	1272:1282	the results of high-throughput sequencing	1272:1312	According to the results of high-throughput sequencing, the diversity of microbial composition in all groups was decreased significantly during chilled storage, especially in the CK group.					
35202469	6	19	theme	composition	1342:1352	arg1	diversity					1319:1327	the diversity	1315:1327	the diversity of microbial composition in all groups	1315:1366	According to the results of high-throughput sequencing, the diversity of microbial composition in all groups was decreased significantly during chilled storage, especially in the CK group.					
35202469	0	20	dep	Fillets	140:146	arg1	Lateolabrax					117:127	Lateolabrax	117:127	Lateolabrax	117:127	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	2	21	theme	%	449:449	arg1	CS					451:452	1% CS	448:452	1% CS	448:452	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	5	22	theme	lipid	1147:1151	arg1	oxidation					1153:1161	lipid oxidation	1147:1161	lipid oxidation	1147:1161	Moreover, CS-g-GA and CS-g-PA performed better in the inhibition of lipid oxidation by the analysis of thiobarbituric acid reactive substances and relative fluorescence intensity.					
35202469	0	23	from	Effects	0:6	arg1	Quality					53:59	Quality	53:59	Quality	53:59	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	0	23	from	Effects	0:6	arg1	Composition					76:86	Microbiota Composition	65:86	Microbiota Composition	65:86	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	0	24	theme	Sea	107:109	arg1	Bass					111:114	Vacuum-Packaged Sea Bass	91:114	Vacuum-Packaged Sea Bass	91:114	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	2	25	theme	%	456:456	arg1	copolymer					466:474	1% CS-g-PA copolymer	455:474	1% CS-g-PA copolymer	455:474	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	5	26	theme	fluorescence	1235:1246	arg1	intensity					1248:1256	relative fluorescence intensity	1226:1256	relative fluorescence intensity	1226:1256	Moreover, CS-g-GA and CS-g-PA performed better in the inhibition of lipid oxidation by the analysis of thiobarbituric acid reactive substances and relative fluorescence intensity.					
35202469	1	27	theme	vacuum-packaged	352:366	arg1	bass					372:375	vacuum-packaged sea bass	352:375	vacuum-packaged sea bass (Lateolabrax japonicus)	352:399	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	1	27	theme	vacuum-packaged	352:366	arg1	japonicus					390:398	Lateolabrax japonicus	378:398	Lateolabrax japonicus	378:398	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	1	28	theme	-grafted	262:269	arg1	derivatives					295:305	chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives	249:305	chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives	249:305	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	5	29	theme	reactive	1202:1209	arg1	substances					1211:1220	thiobarbituric acid reactive substances	1182:1220	thiobarbituric acid reactive substances	1182:1220	Moreover, CS-g-GA and CS-g-PA performed better in the inhibition of lipid oxidation by the analysis of thiobarbituric acid reactive substances and relative fluorescence intensity.					
35202469	0	30	theme	Chitosan-Grafted-Phenolic	11:35	arg1	Coating					42:48	Chitosan-Grafted-Phenolic Acid Coating	11:48	Chitosan-Grafted-Phenolic Acid Coating	11:48	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	1	31	theme	acid	280:283	arg1	derivatives					295:305	chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives	249:305	chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives	249:305	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	7	32	from	Acinetobacter	1482:1494	arg1	period					1510:1515	the middle period	1499:1515	the middle period of storage	1499:1526	The predominant microorganism was Acinetobacter in the middle period of storage, while Pseudomonas and Shewanella became predominant at the end of storage.					
35202469	7	33	theme	predominant	1452:1462	arg1	Acinetobacter					1482:1494	Acinetobacter	1482:1494	Acinetobacter	1482:1494	The predominant microorganism was Acinetobacter in the middle period of storage, while Pseudomonas and Shewanella became predominant at the end of storage.					
35202469	7	33	theme	predominant	1452:1462	arg1	microorganism					1464:1476	The predominant microorganism	1448:1476	The predominant microorganism	1448:1476	The predominant microorganism was Acinetobacter in the middle period of storage, while Pseudomonas and Shewanella became predominant at the end of storage.					
35202469	1	34	dep	quality	314:320	arg1	the					310:312	the	310:312	the	310:312	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	0	35	theme	Coating	42:48	arg1	Effects					0:6	Effects	0:6	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass	0:114	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	9	36	theme	microorganism	1753:1765	arg1	analysis					1737:1744	the analysis	1733:1744	the analysis of the microorganism and physicochemical quality	1733:1793	On the basis of the analysis of the microorganism and physicochemical quality, compared with the CK group, CS-g-GA and CS-g-PA can maintain the good quality of sea bass fillets and prolong the shelf life for another 12 days.					
35202469	0	37	theme	Microbiota	65:74	arg1	Composition					76:86	Microbiota Composition	65:86	Microbiota Composition	65:86	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	1	38	theme	derivatives	295:305	arg1	effect					239:244	the effect	235:244	the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus)	235:399	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	9	39	theme	physicochemical	1771:1785	arg1	quality					1787:1793	physicochemical quality	1771:1793	physicochemical quality	1771:1793	On the basis of the analysis of the microorganism and physicochemical quality, compared with the CK group, CS-g-GA and CS-g-PA can maintain the good quality of sea bass fillets and prolong the shelf life for another 12 days.					
35202469	3	40	theme	volatile	893:900	arg1	nitrogen					908:915	total volatile basic nitrogen	887:915	total volatile basic nitrogen	887:915	The results showed that the treatment of CS-g-GA and CS-g-PA could retard the increase of pH, total volatile basic nitrogen, and the K value.					
35202469	4	41	theme	biogenic	991:998	arg1	amines					1000:1005	biogenic amines	991:1005	biogenic amines	991:1005	The degradation of ATP-related compounds, production of biogenic amines, and growth of spoilage bacteria were inhibited by CS-g-GA and CS-g-PA.					
35202469	6	42	theme	chilled	1403:1409	arg1	storage					1411:1417	chilled storage	1403:1417	chilled storage	1403:1417	According to the results of high-throughput sequencing, the diversity of microbial composition in all groups was decreased significantly during chilled storage, especially in the CK group.					
35202469	6	43	theme	high-throughput	1287:1301	arg1	sequencing					1303:1312	high-throughput sequencing	1287:1312	high-throughput sequencing	1287:1312	According to the results of high-throughput sequencing, the diversity of microbial composition in all groups was decreased significantly during chilled storage, especially in the CK group.					
35202469	2	44	theme	derivatives	743:753	arg1	effects					729:735	the effects	725:735	the effects of CS derivatives on quality and microbial composition	725:790	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	4	45	theme	spoilage	1022:1029	arg1	bacteria					1031:1038	spoilage bacteria	1022:1038	spoilage bacteria	1022:1038	The degradation of ATP-related compounds, production of biogenic amines, and growth of spoilage bacteria were inhibited by CS-g-GA and CS-g-PA.					
35202469	8	46	theme	CS-g-GA	1621:1627	arg1	treatment					1608:1616	The treatment	1604:1616	The treatment of CS-g-GA and CS-g-PA	1604:1639	The treatment of CS-g-GA and CS-g-PA had significant effects inhibiting the growth of Shewanella during storage.					
35202469	8	47	theme	CS-g-PA	1633:1639	arg1	treatment					1608:1616	The treatment	1604:1616	The treatment of CS-g-GA and CS-g-PA	1604:1639	The treatment of CS-g-GA and CS-g-PA had significant effects inhibiting the growth of Shewanella during storage.					
35202469	3	48	theme	K	926:926	arg1	value					928:932	the K value	922:932	the K value	922:932	The results showed that the treatment of CS-g-GA and CS-g-PA could retard the increase of pH, total volatile basic nitrogen, and the K value.					
35202469	8	49	theme	significant	1645:1655	arg1	effects					1657:1663	significant effects	1645:1663	significant effects inhibiting the growth of Shewanella during storage	1645:1714	The treatment of CS-g-GA and CS-g-PA had significant effects inhibiting the growth of Shewanella during storage.					
35202469	1	50	theme	research	197:204	arg1	aim					185:187	The aim	181:187	The aim of this research	181:204	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	2	51	theme	1	481:481	arg1	%					482:482	%	482:482	%	482:482	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	2	52	theme	high-throughput	687:701	arg1	sequencing					703:712	RNA high-throughput sequencing	683:712	RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition	683:790	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	2	53	theme	physicochemical	622:636	arg1	indicators					638:647	the microbiological and physicochemical indicators	598:647	the microbiological and physicochemical indicators	598:647	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	1	54	from	effect	239:244	arg1	quality					314:320	quality	314:320	quality	314:320	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	1	54	from	effect	239:244	arg1	composition					337:347	microbiota composition	326:347	microbiota composition	326:347	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	2	55	theme	acid	502:505	arg1	copolymer					517:525	1% CS-grafted gallic acid (CS-g-GA) copolymer	481:525	1% CS-grafted gallic acid (CS-g-GA) copolymer	481:525	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	9	56	theme	sea	1877:1879	arg1	fillets					1886:1892	sea bass fillets	1877:1892	sea bass fillets	1877:1892	On the basis of the analysis of the microorganism and physicochemical quality, compared with the CK group, CS-g-GA and CS-g-PA can maintain the good quality of sea bass fillets and prolong the shelf life for another 12 days.					
35202469	8	57	theme	Shewanella	1690:1699	arg1	growth					1680:1685	the growth	1676:1685	the growth of Shewanella	1676:1699	The treatment of CS-g-GA and CS-g-PA had significant effects inhibiting the growth of Shewanella during storage.					
35202469	2	58	theme	CS-grafted	484:493	arg1	CS-g-GA					508:514	CS-g-GA	508:514	CS-g-GA	508:514	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	2	58	theme	CS-grafted	484:493	arg1	acid					502:505	CS-grafted gallic acid	484:505	1% CS-grafted gallic acid (CS-g-GA) copolymer	481:525	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	9	59	theme	fillets	1886:1892	arg1	quality					1866:1872	the good quality	1857:1872	the good quality of sea bass fillets	1857:1892	On the basis of the analysis of the microorganism and physicochemical quality, compared with the CK group, CS-g-GA and CS-g-PA can maintain the good quality of sea bass fillets and prolong the shelf life for another 12 days.					
35202469	6	60	theme	CK	1438:1439	arg1	group					1441:1445	the CK group	1434:1445	the CK group	1434:1445	According to the results of high-throughput sequencing, the diversity of microbial composition in all groups was decreased significantly during chilled storage, especially in the CK group.					
35202469	7	61	theme	middle	1503:1508	arg1	period					1510:1515	the middle period	1499:1515	the middle period of storage	1499:1526	The predominant microorganism was Acinetobacter in the middle period of storage, while Pseudomonas and Shewanella became predominant at the end of storage.					
35202469	0	62	theme	Vacuum-Packaged	91:105	arg1	Bass					111:114	Vacuum-Packaged Sea Bass	91:114	Vacuum-Packaged Sea Bass	91:114	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	2	63	theme	1	448:448	arg1	%					449:449	%	449:449	%	449:449	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	9	64	theme	shelf	1910:1914	arg1	life					1916:1919	the shelf life	1906:1919	the shelf life for another 12 days	1906:1939	On the basis of the analysis of the microorganism and physicochemical quality, compared with the CK group, CS-g-GA and CS-g-PA can maintain the good quality of sea bass fillets and prolong the shelf life for another 12 days.					
35202469	5	65	theme	oxidation	1153:1161	arg1	inhibition					1133:1142	the inhibition	1129:1142	the inhibition of lipid oxidation by the analysis of thiobarbituric acid reactive substances and relative fluorescence intensity	1129:1256	Moreover, CS-g-GA and CS-g-PA performed better in the inhibition of lipid oxidation by the analysis of thiobarbituric acid reactive substances and relative fluorescence intensity.					
35202469	0	66	theme	Bass	111:114	arg1	Quality					53:59	Quality	53:59	Quality	53:59	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	0	66	theme	Bass	111:114	arg1	Composition					76:86	Microbiota Composition	65:86	Microbiota Composition	65:86	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	1	67	theme	microbiota	326:335	arg1	composition					337:347	microbiota composition	326:347	microbiota composition	326:347	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	0	68	dep	Lateolabrax	117:127	arg1	japonicus					129:137	Lateolabrax japonicus	117:137	Lateolabrax japonicus	117:137	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	2	69	theme	CS-g-PA	458:464	arg1	copolymer					466:474	1% CS-g-PA copolymer	455:474	1% CS-g-PA copolymer	455:474	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	2	70	theme	vacuum	556:561	arg1	packaging					563:571	vacuum packaging	556:571	vacuum packaging stored at 4°C	556:585	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	5	71	theme	relative	1226:1233	arg1	intensity					1248:1256	relative fluorescence intensity	1226:1256	relative fluorescence intensity	1226:1256	Moreover, CS-g-GA and CS-g-PA performed better in the inhibition of lipid oxidation by the analysis of thiobarbituric acid reactive substances and relative fluorescence intensity.					
35202469	8	72	contain	had	1641:1643	arg2	effects					1657:1663	significant effects	1645:1663	significant effects inhibiting the growth of Shewanella during storage	1645:1714	The treatment of CS-g-GA and CS-g-PA had significant effects inhibiting the growth of Shewanella during storage.					
35202469	8	72	contain	had	1641:1643	arg1	treatment					1608:1616	The treatment	1604:1616	The treatment of CS-g-GA and CS-g-PA	1604:1639	The treatment of CS-g-GA and CS-g-PA had significant effects inhibiting the growth of Shewanella during storage.					
35202469	3	73	theme	CS-g-GA	834:840	arg1	treatment					821:829	the treatment	817:829	the treatment of CS-g-GA and CS-g-PA	817:852	The results showed that the treatment of CS-g-GA and CS-g-PA could retard the increase of pH, total volatile basic nitrogen, and the K value.					
35202469	2	74	theme	1	455:455	arg1	%					456:456	%	456:456	%	456:456	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	2	75	from	effects	729:735	arg1	composition					780:790	microbial composition	770:790	microbial composition	770:790	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	2	75	from	effects	729:735	arg1	quality					758:764	quality	758:764	quality	758:764	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	5	76	theme	intensity	1248:1256	arg1	analysis					1170:1177	the analysis	1166:1177	the analysis of thiobarbituric acid reactive substances and relative fluorescence intensity	1166:1256	Moreover, CS-g-GA and CS-g-PA performed better in the inhibition of lipid oxidation by the analysis of thiobarbituric acid reactive substances and relative fluorescence intensity.					
35202469	1	77	theme	sea	368:370	arg1	bass					372:375	vacuum-packaged sea bass	352:375	vacuum-packaged sea bass (Lateolabrax japonicus)	352:399	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	1	77	theme	sea	368:370	arg1	japonicus					390:398	Lateolabrax japonicus	378:398	Lateolabrax japonicus	378:398	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	2	78	theme	deionized	426:434	arg1	CK					443:444	CK	443:444	CK	443:444	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	2	78	theme	deionized	426:434	arg1	water					436:440	deionized water	426:440	deionized water (CK)	426:445	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35202469	1	79	dep	ABSTRACT	172:179	arg1	was					206:208	was	206:208	was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus)	206:399	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	5	80	theme	acid	1197:1200	arg1	substances					1211:1220	thiobarbituric acid reactive substances	1182:1220	thiobarbituric acid reactive substances	1182:1220	Moreover, CS-g-GA and CS-g-PA performed better in the inhibition of lipid oxidation by the analysis of thiobarbituric acid reactive substances and relative fluorescence intensity.					
35202469	0	81	theme	Acid	37:40	arg1	Coating					42:48	Chitosan-Grafted-Phenolic Acid Coating	11:48	Chitosan-Grafted-Phenolic Acid Coating	11:48	Effects of Chitosan-Grafted-Phenolic Acid Coating on Quality and Microbiota Composition of Vacuum-Packaged Sea Bass (Lateolabrax japonicus) Fillets during Chilled Storage.					
35202469	1	82	theme	phenolic	271:278	arg1	acid					280:283	phenolic acid	271:283	chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives	249:305	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	1	82	theme	phenolic	271:278	arg1	CS-g-PA					286:292	CS-g-PA	286:292	CS-g-PA	286:292	ABSTRACT The aim of this research was to experimentally assess the effect of chitosan (CS)-grafted phenolic acid (CS-g-PA) derivatives on the quality and microbiota composition of vacuum-packaged sea bass (Lateolabrax japonicus).					
35202469	7	83	theme	storage	1595:1601	arg1	end					1588:1590	the end	1584:1590	the end of storage	1584:1601	The predominant microorganism was Acinetobacter in the middle period of storage, while Pseudomonas and Shewanella became predominant at the end of storage.					
35202469	9	84	theme	good	1861:1864	arg1	quality					1866:1872	the good quality	1857:1872	the good quality of sea bass fillets	1857:1892	On the basis of the analysis of the microorganism and physicochemical quality, compared with the CK group, CS-g-GA and CS-g-PA can maintain the good quality of sea bass fillets and prolong the shelf life for another 12 days.					
35202469	5	85	theme	substances	1211:1220	arg1	analysis					1170:1177	the analysis	1166:1177	the analysis of thiobarbituric acid reactive substances and relative fluorescence intensity	1166:1256	Moreover, CS-g-GA and CS-g-PA performed better in the inhibition of lipid oxidation by the analysis of thiobarbituric acid reactive substances and relative fluorescence intensity.					
35202469	6	86	theme	microbial	1332:1340	arg1	composition					1342:1352	microbial composition	1332:1352	microbial composition	1332:1352	According to the results of high-throughput sequencing, the diversity of microbial composition in all groups was decreased significantly during chilled storage, especially in the CK group.					
35202469	3	87	theme	CS-g-PA	846:852	arg1	treatment					821:829	the treatment	817:829	the treatment of CS-g-GA and CS-g-PA	817:852	The results showed that the treatment of CS-g-GA and CS-g-PA could retard the increase of pH, total volatile basic nitrogen, and the K value.					
35202469	3	88	theme	pH	883:884	arg1	value					928:932	the K value	922:932	the K value	922:932	The results showed that the treatment of CS-g-GA and CS-g-PA could retard the increase of pH, total volatile basic nitrogen, and the K value.					
35202469	3	88	theme	pH	883:884	arg1	nitrogen					908:915	total volatile basic nitrogen	887:915	total volatile basic nitrogen	887:915	The results showed that the treatment of CS-g-GA and CS-g-PA could retard the increase of pH, total volatile basic nitrogen, and the K value.					
35202469	3	88	theme	pH	883:884	arg1	increase					871:878	the increase	867:878	the increase of pH	867:884	The results showed that the treatment of CS-g-GA and CS-g-PA could retard the increase of pH, total volatile basic nitrogen, and the K value.					
35202469	9	89	theme	quality	1787:1793	arg1	analysis					1737:1744	the analysis	1733:1744	the analysis of the microorganism and physicochemical quality	1733:1793	On the basis of the analysis of the microorganism and physicochemical quality, compared with the CK group, CS-g-GA and CS-g-PA can maintain the good quality of sea bass fillets and prolong the shelf life for another 12 days.					
35202469	3	90	theme	basic	902:906	arg1	nitrogen					908:915	total volatile basic nitrogen	887:915	total volatile basic nitrogen	887:915	The results showed that the treatment of CS-g-GA and CS-g-PA could retard the increase of pH, total volatile basic nitrogen, and the K value.					
35202469	9	91	theme	CK	1814:1815	arg1	group					1817:1821	the CK group	1810:1821	the CK group	1810:1821	On the basis of the analysis of the microorganism and physicochemical quality, compared with the CK group, CS-g-GA and CS-g-PA can maintain the good quality of sea bass fillets and prolong the shelf life for another 12 days.					
35202469	2	92	theme	microbial	770:778	arg1	composition					780:790	microbial composition	770:790	microbial composition	770:790	Samples were treated by deionized water (CK), 1% CS, 1% CS-g-PA copolymer, and 1% CS-grafted gallic acid (CS-g-GA) copolymer for 10 min and combined with vacuum packaging stored at 4°C to analyze the microbiological and physicochemical indicators; they were also combined with 16s RNA high-throughput sequencing to explore the effects of CS derivatives on quality and microbial composition.					
35698405	0	0	theme	acid-catalyzed	100:113	arg1	solutions					123:131	acid-catalyzed alcohol solutions	100:131	acid-catalyzed alcohol solutions	100:131	Tailored production of lignin-containing cellulose nanofibrils from sugarcane bagasse pretreated by acid-catalyzed alcohol solutions.					
35698405	1	1	theme	lignin-containing	303:319	arg1	LCNF					344:347	LCNF	344:347	LCNF	344:347	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	1	1	theme	lignin-containing	303:319	arg1	nanofibrils					331:341	lignin-containing cellulose nanofibrils	303:341	lignin-containing cellulose nanofibrils (LCNF)	303:348	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	4	2	theme	chains	905:910	arg1	esterification					877:890	esterification	877:890	esterification of aliphatic chains	877:910	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	3	3	theme	thermal	685:691	arg1	stability					693:701	thermal stability	685:701	thermal stability	685:701	LCNF diameter distribution, crystallinity, zeta potentials and thermal stability were also determined to understand the effects of pretreatment solvent.					
35698405	1	4	theme	cellulose	321:329	arg1	LCNF					344:347	LCNF	344:347	LCNF	344:347	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	1	4	theme	cellulose	321:329	arg1	nanofibrils					331:341	lignin-containing cellulose nanofibrils	303:341	lignin-containing cellulose nanofibrils (LCNF)	303:348	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	2	5	theme	highest	480:486	arg1	content					495:501	the highest lignin content	476:501	the highest lignin content of 16%	476:508	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	3	6	theme	LCNF	622:625	arg1	distribution					636:647	LCNF diameter distribution	622:647	LCNF diameter distribution	622:647	LCNF diameter distribution, crystallinity, zeta potentials and thermal stability were also determined to understand the effects of pretreatment solvent.					
35698405	0	7	theme	alcohol	115:121	arg1	solutions					123:131	acid-catalyzed alcohol solutions	100:131	acid-catalyzed alcohol solutions	100:131	Tailored production of lignin-containing cellulose nanofibrils from sugarcane bagasse pretreated by acid-catalyzed alcohol solutions.					
35698405	0	8	from	sugarcane	68:76	arg1	production					9:18	Tailored production	0:18	Tailored production of lignin-containing cellulose nanofibrils from sugarcane	0:76	Tailored production of lignin-containing cellulose nanofibrils from sugarcane bagasse pretreated by acid-catalyzed alcohol solutions.					
35698405	2	9	theme	lignin	568:573	arg1	content					575:581	a low lignin content	562:581	a low lignin content of 11%	562:588	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	1	10	dep	hydrophilicity/hydrophobicity	384:412	arg1	stability					429:437	stability	429:437	stability	429:437	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	4	11	theme	LCNF	969:972	arg1	processes					985:993	the LCNF production processes	965:993	the LCNF production processes	965:993	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	4	11	theme	LCNF	969:972	arg1	properties					1001:1010	LCNF properties	996:1010	LCNF properties	996:1010	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	4	11	theme	LCNF	969:972	arg1	dispersion					1021:1030	LCNF dispersion	1016:1030	LCNF dispersion in different solvents	1016:1052	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	5	12	theme	tailored	1117:1124	arg1	production					1126:1135	tailored production	1117:1135	tailored production	1117:1135	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	2	13	theme	highest	598:604	arg1	hydrophobicity					606:619	the highest hydrophobicity	594:619	the highest hydrophobicity	594:619	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	1	14	theme	acid-catalyzed	187:200	arg1	alcohols					202:209	acid-catalyzed alcohols	187:209	acid-catalyzed alcohols	187:209	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	5	15	theme	LCNF	1140:1143	arg1	production					1126:1135	tailored production	1117:1135	tailored production	1117:1135	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	5	15	theme	LCNF	1140:1143	arg1	design					1106:1111	design	1106:1111	design	1106:1111	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	5	16	from	information	1087:1097	arg1	production					1126:1135	tailored production	1117:1135	tailored production	1117:1135	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	5	16	from	information	1087:1097	arg1	design					1106:1111	design	1106:1111	design	1106:1111	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	4	17	theme	different	1035:1043	arg1	solvents					1045:1052	different solvents	1035:1052	different solvents	1035:1052	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	5	18	theme	manufacturing	1179:1191	arg1	composites					1201:1210	manufacturing polymer composites	1179:1210	manufacturing polymer composites	1179:1210	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	0	19	theme	Tailored	0:7	arg1	production					9:18	Tailored production	0:18	Tailored production of lignin-containing cellulose nanofibrils from sugarcane	0:76	Tailored production of lignin-containing cellulose nanofibrils from sugarcane bagasse pretreated by acid-catalyzed alcohol solutions.					
35698405	5	20	theme	polymer	1193:1199	arg1	composites					1201:1210	manufacturing polymer composites	1179:1210	manufacturing polymer composites	1179:1210	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	2	21	theme	%	588:588	arg1	content					575:581	a low lignin content	562:581	a low lignin content of 11%	562:588	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	4	22	from	oxidation	943:951	arg1	processes					985:993	the LCNF production processes	965:993	the LCNF production processes	965:993	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	4	22	from	oxidation	943:951	arg1	properties					1001:1010	LCNF properties	996:1010	LCNF properties	996:1010	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	4	22	from	oxidation	943:951	arg1	dispersion					1021:1030	LCNF dispersion	1016:1030	LCNF dispersion in different solvents	1016:1052	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	0	23	theme	lignin-containing	23:39	arg1	nanofibrils					51:61	lignin-containing cellulose nanofibrils	23:61	lignin-containing cellulose nanofibrils	23:61	Tailored production of lignin-containing cellulose nanofibrils from sugarcane bagasse pretreated by acid-catalyzed alcohol solutions.					
35698405	4	24	theme	lignin	936:941	arg1	oxidation					943:951	lignin oxidation	936:951	lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents	936:1052	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	2	25	contain	had	472:474	arg2	content					495:501	the highest lignin content	476:501	the highest lignin content of 16%	476:508	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	2	25	contain	had	472:474	arg1	AG-LCNF					464:470	AG-LCNF	464:470	AG-LCNF	464:470	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	2	25	contain	had	472:474	arg2	hydrophobicity					529:542	relatively low hydrophobicity	514:542	relatively low hydrophobicity	514:542	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	2	26	contain	had	558:560	arg2	hydrophobicity					606:619	the highest hydrophobicity	594:619	the highest hydrophobicity	594:619	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	2	26	contain	had	558:560	arg2	content					575:581	a low lignin content	562:581	a low lignin content of 11%	562:588	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	2	26	contain	had	558:560	arg1	AE-LCNF					550:556	AE-LCNF	550:556	AE-LCNF	550:556	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	1	27	theme	tailored	355:362	arg1	hydrophilicity/hydrophobicity					384:412	hydrophilicity/hydrophobicity	384:412	hydrophilicity/hydrophobicity	384:412	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	1	27	theme	tailored	355:362	arg1	properties					364:373	tailored properties	355:373	tailored properties	355:373	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	1	27	theme	tailored	355:362	arg1	dispersion					418:427	dispersion	418:427	dispersion	418:427	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	0	28	dep	bagasse	78:84	arg1	pretreated					86:95	pretreated	86:95	bagasse pretreated by acid-catalyzed alcohol solutions	78:131	Tailored production of lignin-containing cellulose nanofibrils from sugarcane bagasse pretreated by acid-catalyzed alcohol solutions.					
35698405	0	29	theme	nanofibrils	51:61	arg1	production					9:18	Tailored production	0:18	Tailored production of lignin-containing cellulose nanofibrils from sugarcane	0:76	Tailored production of lignin-containing cellulose nanofibrils from sugarcane bagasse pretreated by acid-catalyzed alcohol solutions.					
35698405	5	30	theme	Pickering	1216:1224	arg1	emulsions					1226:1234	Pickering emulsions	1216:1234	Pickering emulsions	1216:1234	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	2	31	theme	low	525:527	arg1	hydrophobicity					529:542	relatively low hydrophobicity	514:542	relatively low hydrophobicity	514:542	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	3	32	theme	diameter	627:634	arg1	distribution					636:647	LCNF diameter distribution	622:647	LCNF diameter distribution	622:647	LCNF diameter distribution, crystallinity, zeta potentials and thermal stability were also determined to understand the effects of pretreatment solvent.					
35698405	0	33	theme	cellulose	41:49	arg1	nanofibrils					51:61	lignin-containing cellulose nanofibrils	23:61	lignin-containing cellulose nanofibrils	23:61	Tailored production of lignin-containing cellulose nanofibrils from sugarcane bagasse pretreated by acid-catalyzed alcohol solutions.					
35698405	3	34	theme	solvent	766:772	arg1	effects					742:748	the effects	738:748	the effects of pretreatment solvent	738:772	LCNF diameter distribution, crystallinity, zeta potentials and thermal stability were also determined to understand the effects of pretreatment solvent.					
35698405	4	35	from	properties	1001:1010	arg1	solvents					1045:1052	different solvents	1035:1052	different solvents	1035:1052	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	4	36	from	dispersion	1021:1030	arg1	solvents					1045:1052	different solvents	1035:1052	different solvents	1035:1052	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	2	37	theme	low	564:566	arg1	content					575:581	a low lignin content	562:581	a low lignin content of 11%	562:588	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	4	38	theme	production	974:983	arg1	processes					985:993	the LCNF production processes	965:993	the LCNF production processes	965:993	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	4	38	theme	production	974:983	arg1	properties					1001:1010	LCNF properties	996:1010	LCNF properties	996:1010	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	4	38	theme	production	974:983	arg1	dispersion					1021:1030	LCNF dispersion	1016:1030	LCNF dispersion in different solvents	1016:1052	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	5	39	theme	various	1149:1155	arg1	composites					1201:1210	manufacturing polymer composites	1179:1210	manufacturing polymer composites	1179:1210	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	5	39	theme	various	1149:1155	arg1	emulsions					1226:1234	Pickering emulsions	1216:1234	Pickering emulsions	1216:1234	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	5	39	theme	various	1149:1155	arg1	applications					1157:1168	various applications	1149:1168	various applications	1149:1168	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	4	40	theme	LCNF	1016:1019	arg1	processes					985:993	the LCNF production processes	965:993	the LCNF production processes	965:993	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	4	40	theme	LCNF	1016:1019	arg1	dispersion					1021:1030	LCNF dispersion	1016:1030	LCNF dispersion in different solvents	1016:1052	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	4	41	theme	aliphatic	895:903	arg1	chains					905:910	aliphatic chains	895:910	aliphatic chains	895:910	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	3	42	theme	pretreatment	753:764	arg1	solvent					766:772	pretreatment solvent	753:772	pretreatment solvent	753:772	LCNF diameter distribution, crystallinity, zeta potentials and thermal stability were also determined to understand the effects of pretreatment solvent.					
35698405	3	43	theme	zeta	665:668	arg1	potentials					670:679	zeta potentials	665:679	zeta potentials	665:679	LCNF diameter distribution, crystallinity, zeta potentials and thermal stability were also determined to understand the effects of pretreatment solvent.					
35698405	4	44	theme	NMR	775:777	arg1	analyses					779:786	NMR analyses	775:786	NMR analyses	775:786	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	1	45	theme	sugarcane	149:157	arg1	bagasse					159:165	sugarcane bagasse	149:165	sugarcane bagasse	149:165	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	5	46	dep	design	1106:1111	arg1	the					1102:1104	the	1102:1104	the	1102:1104	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	5	47	theme	fundamental	1075:1085	arg1	information					1087:1097	fundamental information	1075:1097	fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions	1075:1234	This study provided fundamental information in the design and tailored production of LCNF for various applications, such as manufacturing polymer composites and Pickering emulsions.					
35698405	1	48	theme	ethylene	232:239	arg1	AEG					249:251	AEG	249:251	AEG	249:251	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	1	48	theme	ethylene	232:239	arg1	glycol					241:246	ethylene glycol	232:246	ethylene glycol (AEG)	232:252	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	2	49	theme	lignin	488:493	arg1	content					495:501	the highest lignin content	476:501	the highest lignin content of 16%	476:508	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
35698405	4	50	theme	LCNF	996:999	arg1	processes					985:993	the LCNF production processes	965:993	the LCNF production processes	965:993	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	4	50	theme	LCNF	996:999	arg1	properties					1001:1010	LCNF properties	996:1010	LCNF properties	996:1010	NMR analyses revealed that alcohols modified lignin at α-position by etherification and γ-position by esterification of aliphatic chains, subsequently affecting lignin oxidation by TEMPO in the LCNF production processes, LCNF properties and LCNF dispersion in different solvents.					
35698405	1	51	dep	ethanol	218:224	arg1	i.e.					212:215	i.e.	212:215	i.e.	212:215	In this study, sugarcane bagasse was pretreated with acid-catalyzed alcohols, i.e., ethanol (AE), ethylene glycol (AEG) and glycerol (AG) to prepare pulps for producing lignin-containing cellulose nanofibrils (LCNF) with tailored properties, such as hydrophilicity/hydrophobicity and dispersion stability.					
35698405	2	52	theme	%	508:508	arg1	content					495:501	the highest lignin content	476:501	the highest lignin content of 16%	476:508	The results showed that AG-LCNF had the highest lignin content of 16% but relatively low hydrophobicity while AE-LCNF had a low lignin content of 11% but the highest hydrophobicity.					
36321923	6	0	theme	synergistic	965:975	arg1	effect					977:982	the synergistic effect	961:982	the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin	961:1034	In this process, due to the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin, the metabolism of bacteria was well inhibited, and the efficient capturing and rapid killing of bacteria were achieved.					
36321923	4	1	theme	radical	769:775	arg1	polymerization					777:790	radical polymerization	769:790	radical polymerization	769:790	Multifunctional composite hydrogels were conveniently synthesized by combining methacrylic anhydride modified gelatin and methacrylic anhydride modified oxidized sodium alginate with QCSMOF-Van through radical polymerization and Schiff base reaction.					
36321923	8	2	from	potential	1452:1460	arg1	dressings					1518:1526	chronic wound dressings	1504:1526	chronic wound dressings	1504:1526	This advanced cascade management strategy for tissue regeneration highlights the potential of multifunctional composite hydrogels in chronic wound dressings.					
36321923	7	3	theme	vessels	1310:1316	arg1	regeneration					1277:1288	the regeneration	1273:1288	the regeneration of nerves and blood vessels, which promotes the rapid healing of chronic wounds	1273:1368	The QCSMOF-Van hydrogels could precisely regulate the balance of M1/M2 phenotypes of macrophages, thereby promoting the regeneration of nerves and blood vessels, which promotes the rapid healing of chronic wounds.					
36321923	6	4	theme	bacteria	1055:1062	arg1	metabolism					1041:1050	the metabolism	1037:1050	the metabolism of bacteria	1037:1062	In this process, due to the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin, the metabolism of bacteria was well inhibited, and the efficient capturing and rapid killing of bacteria were achieved.					
36321923	8	5	theme	chronic	1504:1510	arg1	dressings					1518:1526	chronic wound dressings	1504:1526	chronic wound dressings	1504:1526	This advanced cascade management strategy for tissue regeneration highlights the potential of multifunctional composite hydrogels in chronic wound dressings.					
36321923	7	6	theme	QCSMOF-Van	1161:1170	arg1	hydrogels					1172:1180	The QCSMOF-Van hydrogels	1157:1180	The QCSMOF-Van hydrogels	1157:1180	The QCSMOF-Van hydrogels could precisely regulate the balance of M1/M2 phenotypes of macrophages, thereby promoting the regeneration of nerves and blood vessels, which promotes the rapid healing of chronic wounds.					
36321923	2	7	theme	favorable	375:383	arg1	microenvironment					385:400	a favorable microenvironment	373:400	a favorable microenvironment	373:400	Therefore, an urgent desire is to develop a multifunctional wound dressing that can prevent wound infection and promote wound healing by creating a favorable microenvironment.					
36321923	2	8	theme	wound	347:351	arg1	healing					353:359	wound healing	347:359	wound healing	347:359	Therefore, an urgent desire is to develop a multifunctional wound dressing that can prevent wound infection and promote wound healing by creating a favorable microenvironment.					
36321923	3	9	theme	quaternary	512:521	arg1	chitosan					537:544	quaternary ammonium salt chitosan	512:544	quaternary ammonium salt chitosan (QCS)	512:550	In this study, a curcumin-based metal-organic framework (QCSMOF-Van), loaded with vancomycin and coated with quaternary ammonium salt chitosan (QCS), was prepared.					
36321923	3	9	theme	quaternary	512:521	arg1	QCS					547:549	QCS	547:549	QCS	547:549	In this study, a curcumin-based metal-organic framework (QCSMOF-Van), loaded with vancomycin and coated with quaternary ammonium salt chitosan (QCS), was prepared.					
36321923	6	10	theme	bacteria	1133:1140	arg1	killing					1122:1128	the efficient capturing and rapid killing	1088:1128	the efficient capturing and rapid killing of bacteria	1088:1140	In this process, due to the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin, the metabolism of bacteria was well inhibited, and the efficient capturing and rapid killing of bacteria were achieved.					
36321923	4	11	theme	oxidized	720:727	arg1	alginate					736:743	oxidized sodium alginate	720:743	oxidized sodium alginate	720:743	Multifunctional composite hydrogels were conveniently synthesized by combining methacrylic anhydride modified gelatin and methacrylic anhydride modified oxidized sodium alginate with QCSMOF-Van through radical polymerization and Schiff base reaction.					
36321923	1	12	theme	Chronic	107:113	arg1	wounds					115:120	Chronic wounds	107:120	Chronic wounds	107:120	Chronic wounds are characterized by long-term inflammation and persistent infection, which make them difficult to heal.					
36321923	5	13	theme	QCS	932:934	arg1	surface					921:927	the surface	917:927	the surface of QCS	917:934	It is important to note that the QCSMOF-Van could capture bacteria through the positive charges on the surface of QCS.					
36321923	0	14	theme	Photothermal	0:11	arg1	Hydrogel					13:20	Photothermal Hydrogel	0:20	Photothermal Hydrogel	0:20	Photothermal Hydrogel Encapsulating Intelligently Bacteria-Capturing Bio-MOF for Infectious Wound Healing.					
36321923	4	15	theme	Multifunctional	567:581	arg1	hydrogels					593:601	Multifunctional composite hydrogels	567:601	Multifunctional composite hydrogels	567:601	Multifunctional composite hydrogels were conveniently synthesized by combining methacrylic anhydride modified gelatin and methacrylic anhydride modified oxidized sodium alginate with QCSMOF-Van through radical polymerization and Schiff base reaction.					
36321923	3	16	dep	framework	449:457	arg1	coated					500:505	coated	500:505	coated with quaternary ammonium salt chitosan (QCS)	500:550	In this study, a curcumin-based metal-organic framework (QCSMOF-Van), loaded with vancomycin and coated with quaternary ammonium salt chitosan (QCS), was prepared.					
36321923	3	16	dep	framework	449:457	arg1	loaded					473:478	loaded	473:478	loaded with vancomycin	473:494	In this study, a curcumin-based metal-organic framework (QCSMOF-Van), loaded with vancomycin and coated with quaternary ammonium salt chitosan (QCS), was prepared.					
36321923	4	17	theme	sodium	729:734	arg1	alginate					736:743	oxidized sodium alginate	720:743	oxidized sodium alginate	720:743	Multifunctional composite hydrogels were conveniently synthesized by combining methacrylic anhydride modified gelatin and methacrylic anhydride modified oxidized sodium alginate with QCSMOF-Van through radical polymerization and Schiff base reaction.					
36321923	0	18	theme	Intelligently	36:48	arg1	Bio-MOF					69:75	Intelligently Bacteria-Capturing Bio-MOF	36:75	Intelligently Bacteria-Capturing Bio-MOF for Infectious Wound Healing	36:104	Photothermal Hydrogel Encapsulating Intelligently Bacteria-Capturing Bio-MOF for Infectious Wound Healing.					
36321923	8	19	theme	hydrogels	1491:1499	arg1	potential					1452:1460	the potential	1448:1460	the potential of multifunctional composite hydrogels in chronic wound dressings	1448:1526	This advanced cascade management strategy for tissue regeneration highlights the potential of multifunctional composite hydrogels in chronic wound dressings.					
36321923	8	20	theme	cascade	1385:1391	arg1	strategy					1404:1411	This advanced cascade management strategy	1371:1411	This advanced cascade management strategy for tissue regeneration	1371:1435	This advanced cascade management strategy for tissue regeneration highlights the potential of multifunctional composite hydrogels in chronic wound dressings.					
36321923	7	21	theme	wounds	1363:1368	arg1	healing					1344:1350	the rapid healing	1334:1350	the rapid healing of chronic wounds	1334:1368	The QCSMOF-Van hydrogels could precisely regulate the balance of M1/M2 phenotypes of macrophages, thereby promoting the regeneration of nerves and blood vessels, which promotes the rapid healing of chronic wounds.					
36321923	5	22	theme	positive	897:904	arg1	charges					906:912	the positive charges	893:912	the positive charges on the surface of QCS	893:934	It is important to note that the QCSMOF-Van could capture bacteria through the positive charges on the surface of QCS.					
36321923	6	23	theme	vancomycin	1025:1034	arg1	effect					977:982	the synergistic effect	961:982	the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin	961:1034	In this process, due to the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin, the metabolism of bacteria was well inhibited, and the efficient capturing and rapid killing of bacteria were achieved.					
36321923	2	24	theme	wound	319:323	arg1	infection					325:333	wound infection	319:333	wound infection	319:333	Therefore, an urgent desire is to develop a multifunctional wound dressing that can prevent wound infection and promote wound healing by creating a favorable microenvironment.					
36321923	6	25	theme	capturing	1102:1110	arg1	killing					1122:1128	the efficient capturing and rapid killing	1088:1128	the efficient capturing and rapid killing of bacteria	1088:1140	In this process, due to the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin, the metabolism of bacteria was well inhibited, and the efficient capturing and rapid killing of bacteria were achieved.					
36321923	3	26	theme	metal-organic	435:447	arg1	QCSMOF-Van					460:469	QCSMOF-Van	460:469	QCSMOF-Van	460:469	In this study, a curcumin-based metal-organic framework (QCSMOF-Van), loaded with vancomycin and coated with quaternary ammonium salt chitosan (QCS), was prepared.					
36321923	3	26	theme	metal-organic	435:447	arg1	framework					449:457	a curcumin-based metal-organic framework	418:457	a curcumin-based metal-organic framework (QCSMOF-Van)	418:470	In this study, a curcumin-based metal-organic framework (QCSMOF-Van), loaded with vancomycin and coated with quaternary ammonium salt chitosan (QCS), was prepared.					
36321923	4	27	theme	composite	583:591	arg1	hydrogels					593:601	Multifunctional composite hydrogels	567:601	Multifunctional composite hydrogels	567:601	Multifunctional composite hydrogels were conveniently synthesized by combining methacrylic anhydride modified gelatin and methacrylic anhydride modified oxidized sodium alginate with QCSMOF-Van through radical polymerization and Schiff base reaction.					
36321923	7	28	theme	macrophages	1242:1252	arg1	phenotypes					1228:1237	M1/M2 phenotypes	1222:1237	M1/M2 phenotypes of macrophages	1222:1252	The QCSMOF-Van hydrogels could precisely regulate the balance of M1/M2 phenotypes of macrophages, thereby promoting the regeneration of nerves and blood vessels, which promotes the rapid healing of chronic wounds.					
36321923	8	29	theme	composite	1481:1489	arg1	hydrogels					1491:1499	multifunctional composite hydrogels	1465:1499	multifunctional composite hydrogels	1465:1499	This advanced cascade management strategy for tissue regeneration highlights the potential of multifunctional composite hydrogels in chronic wound dressings.					
36321923	6	30	theme	rapid	1116:1120	arg1	killing					1122:1128	the efficient capturing and rapid killing	1088:1128	the efficient capturing and rapid killing of bacteria	1088:1140	In this process, due to the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin, the metabolism of bacteria was well inhibited, and the efficient capturing and rapid killing of bacteria were achieved.					
36321923	6	31	theme	efficient	1092:1100	arg1	killing					1122:1128	the efficient capturing and rapid killing	1088:1128	the efficient capturing and rapid killing of bacteria	1088:1140	In this process, due to the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin, the metabolism of bacteria was well inhibited, and the efficient capturing and rapid killing of bacteria were achieved.					
36321923	0	32	theme	Bacteria-Capturing	50:67	arg1	Bio-MOF					69:75	Intelligently Bacteria-Capturing Bio-MOF	36:75	Intelligently Bacteria-Capturing Bio-MOF for Infectious Wound Healing	36:104	Photothermal Hydrogel Encapsulating Intelligently Bacteria-Capturing Bio-MOF for Infectious Wound Healing.					
36321923	6	33	theme	Zn2+	1016:1019	arg1	effect					977:982	the synergistic effect	961:982	the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin	961:1034	In this process, due to the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin, the metabolism of bacteria was well inhibited, and the efficient capturing and rapid killing of bacteria were achieved.					
36321923	7	34	theme	M1/M2	1222:1226	arg1	phenotypes					1228:1237	M1/M2 phenotypes	1222:1237	M1/M2 phenotypes of macrophages	1222:1252	The QCSMOF-Van hydrogels could precisely regulate the balance of M1/M2 phenotypes of macrophages, thereby promoting the regeneration of nerves and blood vessels, which promotes the rapid healing of chronic wounds.					
36321923	4	35	theme	methacrylic	689:699	arg1	anhydride					701:709	methacrylic anhydride	689:709	methacrylic anhydride	689:709	Multifunctional composite hydrogels were conveniently synthesized by combining methacrylic anhydride modified gelatin and methacrylic anhydride modified oxidized sodium alginate with QCSMOF-Van through radical polymerization and Schiff base reaction.					
36321923	0	36	theme	Infectious	81:90	arg1	Healing					98:104	Infectious Wound Healing	81:104	Infectious Wound Healing	81:104	Photothermal Hydrogel Encapsulating Intelligently Bacteria-Capturing Bio-MOF for Infectious Wound Healing.					
36321923	3	37	theme	salt	532:535	arg1	chitosan					537:544	quaternary ammonium salt chitosan	512:544	quaternary ammonium salt chitosan (QCS)	512:550	In this study, a curcumin-based metal-organic framework (QCSMOF-Van), loaded with vancomycin and coated with quaternary ammonium salt chitosan (QCS), was prepared.					
36321923	3	37	theme	salt	532:535	arg1	QCS					547:549	QCS	547:549	QCS	547:549	In this study, a curcumin-based metal-organic framework (QCSMOF-Van), loaded with vancomycin and coated with quaternary ammonium salt chitosan (QCS), was prepared.					
36321923	7	38	theme	rapid	1338:1342	arg1	healing					1344:1350	the rapid healing	1334:1350	the rapid healing of chronic wounds	1334:1368	The QCSMOF-Van hydrogels could precisely regulate the balance of M1/M2 phenotypes of macrophages, thereby promoting the regeneration of nerves and blood vessels, which promotes the rapid healing of chronic wounds.					
36321923	1	39	theme	long-term	143:151	arg1	inflammation					153:164	long-term inflammation	143:164	long-term inflammation	143:164	Chronic wounds are characterized by long-term inflammation and persistent infection, which make them difficult to heal.					
36321923	3	40	theme	curcumin-based	420:433	arg1	QCSMOF-Van					460:469	QCSMOF-Van	460:469	QCSMOF-Van	460:469	In this study, a curcumin-based metal-organic framework (QCSMOF-Van), loaded with vancomycin and coated with quaternary ammonium salt chitosan (QCS), was prepared.					
36321923	3	40	theme	curcumin-based	420:433	arg1	framework					449:457	a curcumin-based metal-organic framework	418:457	a curcumin-based metal-organic framework (QCSMOF-Van)	418:470	In this study, a curcumin-based metal-organic framework (QCSMOF-Van), loaded with vancomycin and coated with quaternary ammonium salt chitosan (QCS), was prepared.					
36321923	6	41	theme	broad-spectrum	987:1000	arg1	Zn2+					1016:1019	broad-spectrum antibacterial Zn2+	987:1019	broad-spectrum antibacterial Zn2+	987:1019	In this process, due to the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin, the metabolism of bacteria was well inhibited, and the efficient capturing and rapid killing of bacteria were achieved.					
36321923	2	42	theme	urgent	241:246	arg1	desire					248:253	an urgent desire	238:253	an urgent desire	238:253	Therefore, an urgent desire is to develop a multifunctional wound dressing that can prevent wound infection and promote wound healing by creating a favorable microenvironment.					
36321923	5	43	from	charges	906:912	arg1	surface					921:927	the surface	917:927	the surface of QCS	917:934	It is important to note that the QCSMOF-Van could capture bacteria through the positive charges on the surface of QCS.					
36321923	3	44	theme	ammonium	523:530	arg1	chitosan					537:544	quaternary ammonium salt chitosan	512:544	quaternary ammonium salt chitosan (QCS)	512:550	In this study, a curcumin-based metal-organic framework (QCSMOF-Van), loaded with vancomycin and coated with quaternary ammonium salt chitosan (QCS), was prepared.					
36321923	3	44	theme	ammonium	523:530	arg1	QCS					547:549	QCS	547:549	QCS	547:549	In this study, a curcumin-based metal-organic framework (QCSMOF-Van), loaded with vancomycin and coated with quaternary ammonium salt chitosan (QCS), was prepared.					
36321923	7	45	theme	blood	1304:1308	arg1	vessels					1310:1316	blood vessels	1304:1316	blood vessels	1304:1316	The QCSMOF-Van hydrogels could precisely regulate the balance of M1/M2 phenotypes of macrophages, thereby promoting the regeneration of nerves and blood vessels, which promotes the rapid healing of chronic wounds.					
36321923	8	46	theme	wound	1512:1516	arg1	dressings					1518:1526	chronic wound dressings	1504:1526	chronic wound dressings	1504:1526	This advanced cascade management strategy for tissue regeneration highlights the potential of multifunctional composite hydrogels in chronic wound dressings.					
36321923	2	47	theme	wound	287:291	arg1	dressing					293:300	a multifunctional wound dressing	269:300	a multifunctional wound dressing that can prevent wound infection and promote wound healing by creating a favorable microenvironment	269:400	Therefore, an urgent desire is to develop a multifunctional wound dressing that can prevent wound infection and promote wound healing by creating a favorable microenvironment.					
36321923	8	48	theme	multifunctional	1465:1479	arg1	hydrogels					1491:1499	multifunctional composite hydrogels	1465:1499	multifunctional composite hydrogels	1465:1499	This advanced cascade management strategy for tissue regeneration highlights the potential of multifunctional composite hydrogels in chronic wound dressings.					
36321923	4	49	theme	base	803:806	arg1	reaction					808:815	Schiff base reaction	796:815	Schiff base reaction	796:815	Multifunctional composite hydrogels were conveniently synthesized by combining methacrylic anhydride modified gelatin and methacrylic anhydride modified oxidized sodium alginate with QCSMOF-Van through radical polymerization and Schiff base reaction.					
36321923	4	50	theme	methacrylic	646:656	arg1	anhydride					658:666	methacrylic anhydride	646:666	methacrylic anhydride modified gelatin	646:683	Multifunctional composite hydrogels were conveniently synthesized by combining methacrylic anhydride modified gelatin and methacrylic anhydride modified oxidized sodium alginate with QCSMOF-Van through radical polymerization and Schiff base reaction.					
36321923	8	51	theme	tissue	1417:1422	arg1	regeneration					1424:1435	tissue regeneration	1417:1435	tissue regeneration	1417:1435	This advanced cascade management strategy for tissue regeneration highlights the potential of multifunctional composite hydrogels in chronic wound dressings.					
36321923	2	52	theme	multifunctional	271:285	arg1	dressing					293:300	a multifunctional wound dressing	269:300	a multifunctional wound dressing that can prevent wound infection and promote wound healing by creating a favorable microenvironment	269:400	Therefore, an urgent desire is to develop a multifunctional wound dressing that can prevent wound infection and promote wound healing by creating a favorable microenvironment.					
36321923	1	53	theme	persistent	170:179	arg1	infection					181:189	persistent infection	170:189	persistent infection	170:189	Chronic wounds are characterized by long-term inflammation and persistent infection, which make them difficult to heal.					
36321923	8	54	theme	management	1393:1402	arg1	strategy					1404:1411	This advanced cascade management strategy	1371:1411	This advanced cascade management strategy for tissue regeneration	1371:1435	This advanced cascade management strategy for tissue regeneration highlights the potential of multifunctional composite hydrogels in chronic wound dressings.					
36321923	4	55	theme	Schiff	796:801	arg1	reaction					808:815	Schiff base reaction	796:815	Schiff base reaction	796:815	Multifunctional composite hydrogels were conveniently synthesized by combining methacrylic anhydride modified gelatin and methacrylic anhydride modified oxidized sodium alginate with QCSMOF-Van through radical polymerization and Schiff base reaction.					
36321923	7	56	theme	nerves	1293:1298	arg1	regeneration					1277:1288	the regeneration	1273:1288	the regeneration of nerves and blood vessels, which promotes the rapid healing of chronic wounds	1273:1368	The QCSMOF-Van hydrogels could precisely regulate the balance of M1/M2 phenotypes of macrophages, thereby promoting the regeneration of nerves and blood vessels, which promotes the rapid healing of chronic wounds.					
36321923	7	57	theme	chronic	1355:1361	arg1	wounds					1363:1368	chronic wounds	1355:1368	chronic wounds	1355:1368	The QCSMOF-Van hydrogels could precisely regulate the balance of M1/M2 phenotypes of macrophages, thereby promoting the regeneration of nerves and blood vessels, which promotes the rapid healing of chronic wounds.					
36321923	8	58	theme	advanced	1376:1383	arg1	strategy					1404:1411	This advanced cascade management strategy	1371:1411	This advanced cascade management strategy for tissue regeneration	1371:1435	This advanced cascade management strategy for tissue regeneration highlights the potential of multifunctional composite hydrogels in chronic wound dressings.					
36321923	4	59	theme	modified	668:675	arg1	gelatin					677:683	methacrylic anhydride modified gelatin	646:683	methacrylic anhydride modified gelatin	646:683	Multifunctional composite hydrogels were conveniently synthesized by combining methacrylic anhydride modified gelatin and methacrylic anhydride modified oxidized sodium alginate with QCSMOF-Van through radical polymerization and Schiff base reaction.					
36321923	0	60	theme	Wound	92:96	arg1	Healing					98:104	Infectious Wound Healing	81:104	Infectious Wound Healing	81:104	Photothermal Hydrogel Encapsulating Intelligently Bacteria-Capturing Bio-MOF for Infectious Wound Healing.					
36321923	7	61	theme	phenotypes	1228:1237	arg1	balance					1211:1217	the balance	1207:1217	the balance of M1/M2 phenotypes of macrophages	1207:1252	The QCSMOF-Van hydrogels could precisely regulate the balance of M1/M2 phenotypes of macrophages, thereby promoting the regeneration of nerves and blood vessels, which promotes the rapid healing of chronic wounds.					
36321923	6	62	theme	antibacterial	1002:1014	arg1	Zn2+					1016:1019	broad-spectrum antibacterial Zn2+	987:1019	broad-spectrum antibacterial Zn2+	987:1019	In this process, due to the synergistic effect of broad-spectrum antibacterial Zn2+ and vancomycin, the metabolism of bacteria was well inhibited, and the efficient capturing and rapid killing of bacteria were achieved.					
36321923	4	63	theme	anhydride	658:666	arg1	gelatin					677:683	methacrylic anhydride modified gelatin	646:683	methacrylic anhydride modified gelatin	646:683	Multifunctional composite hydrogels were conveniently synthesized by combining methacrylic anhydride modified gelatin and methacrylic anhydride modified oxidized sodium alginate with QCSMOF-Van through radical polymerization and Schiff base reaction.					
36563820	8	0	theme	low	1360:1362	arg1	cost					1364:1367	low cost	1360:1367	low cost of testing coupled with the app	1360:1399	The portability, ease of use, and low cost of testing coupled with the app, make it an attractive alternative to lab-based analysis.					
36563820	2	1	theme	intelligent	325:335	arg1	bio-films					342:350	selective intelligent thin bio-films	315:350	selective intelligent thin bio-films	315:350	We prepared selective intelligent thin bio-films and a dedicated app to test milk adulteration in an out-of-lab setting.					
36563820	6	2	from	1.0 mg/L	1172:1179	arg1	10 mL					1136:1140	10 mL	1136:1140	10 mL of milk with the LODs of 0.1	1136:1169	These preservatives ranged from 0.1 to 200.0 mg/L, 1.0-100.0 mg/L, 0.001-0.05 mol/L in 10 mL of milk with the LODs of 0.1, 1.0 mg/L, and 0.001 mmol/L, for H2O2, Cr(VI), and salicylic acid, respectively.					
36563820	8	3	dep	it	1407:1408	arg1	alternative					1424:1434	an attractive alternative	1410:1434	it an attractive alternative to lab-based analysis	1407:1456	The portability, ease of use, and low cost of testing coupled with the app, make it an attractive alternative to lab-based analysis.					
36563820	8	4	theme	testing	1372:1378	arg1	ease					1343:1346	ease	1343:1346	ease of use	1343:1353	The portability, ease of use, and low cost of testing coupled with the app, make it an attractive alternative to lab-based analysis.					
36563820	8	4	theme	testing	1372:1378	arg1	portability					1330:1340	The portability	1326:1340	The portability	1326:1340	The portability, ease of use, and low cost of testing coupled with the app, make it an attractive alternative to lab-based analysis.					
36563820	8	4	theme	testing	1372:1378	arg1	cost					1364:1367	low cost	1360:1367	low cost of testing coupled with the app	1360:1399	The portability, ease of use, and low cost of testing coupled with the app, make it an attractive alternative to lab-based analysis.					
36563820	2	5	theme	selective	315:323	arg1	bio-films					342:350	selective intelligent thin bio-films	315:350	selective intelligent thin bio-films	315:350	We prepared selective intelligent thin bio-films and a dedicated app to test milk adulteration in an out-of-lab setting.					
36563820	6	6	with	10 mL	1136:1140	arg1	LODs					1159:1162	the LODs	1155:1162	the LODs of 0.1	1155:1169	These preservatives ranged from 0.1 to 200.0 mg/L, 1.0-100.0 mg/L, 0.001-0.05 mol/L in 10 mL of milk with the LODs of 0.1, 1.0 mg/L, and 0.001 mmol/L, for H2O2, Cr(VI), and salicylic acid, respectively.					
36563820	6	7	theme	milk	1145:1148	arg1	10 mL					1136:1140	10 mL	1136:1140	10 mL of milk with the LODs of 0.1	1136:1169	These preservatives ranged from 0.1 to 200.0 mg/L, 1.0-100.0 mg/L, 0.001-0.05 mol/L in 10 mL of milk with the LODs of 0.1, 1.0 mg/L, and 0.001 mmol/L, for H2O2, Cr(VI), and salicylic acid, respectively.					
36563820	3	8	theme	film	497:500	arg1	piece					473:477	a piece	471:477	a piece of starch/gelatin film	471:500	In this regard, each reagent is immobilized on a piece of starch/gelatin film as color-changeable polymeric support, which is then pasted onto a transparent test card.					
36563820	5	9	theme	Web	879:881	arg1	Application					883:893	a Progressive Web Application	865:893	a Progressive Web Application	865:893	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	4	10	theme	composite	650:658	arg1	films					660:664	these composite films	644:664	these composite films	644:664	Incorporating starch and gelatin as the backbone of these composite films can significantly improve the mechanical properties, color, and potential applications in real-time monitoring.					
36563820	0	11	theme	intelligent	80:90	arg1	film					92:95	starch/gelatin intelligent film	65:95	starch/gelatin intelligent film	65:95	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	5	12	from	floating	790:797	arg1	surface					818:824	the surface	814:824	the surface of the milk	814:836	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	4	13	theme	potential	730:738	arg1	applications					740:751	potential applications	730:751	potential applications	730:751	Incorporating starch and gelatin as the backbone of these composite films can significantly improve the mechanical properties, color, and potential applications in real-time monitoring.					
36563820	6	14	from	1.0-100.0 mg/L	1100:1113	arg1	10 mL					1136:1140	10 mL	1136:1140	10 mL of milk with the LODs of 0.1	1136:1169	These preservatives ranged from 0.1 to 200.0 mg/L, 1.0-100.0 mg/L, 0.001-0.05 mol/L in 10 mL of milk with the LODs of 0.1, 1.0 mg/L, and 0.001 mmol/L, for H2O2, Cr(VI), and salicylic acid, respectively.					
36563820	0	15	theme	starch/gelatin	65:78	arg1	film					92:95	starch/gelatin intelligent film	65:95	starch/gelatin intelligent film	65:95	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	5	16	theme	preservatives	1034:1046	arg1	presence					1017:1024	the presence	1013:1024	the presence of some preservatives	1013:1046	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	3	17	theme	starch/gelatin	482:495	arg1	film					497:500	starch/gelatin film	482:500	starch/gelatin film	482:500	In this regard, each reagent is immobilized on a piece of starch/gelatin film as color-changeable polymeric support, which is then pasted onto a transparent test card.					
36563820	2	18	theme	dedicated	358:366	arg1	app					368:370	a dedicated app	356:370	a dedicated app	356:370	We prepared selective intelligent thin bio-films and a dedicated app to test milk adulteration in an out-of-lab setting.					
36563820	6	19	dep	Cr	1210:1211	arg1	VI					1213:1214	VI	1213:1214	VI	1213:1214	These preservatives ranged from 0.1 to 200.0 mg/L, 1.0-100.0 mg/L, 0.001-0.05 mol/L in 10 mL of milk with the LODs of 0.1, 1.0 mg/L, and 0.001 mmol/L, for H2O2, Cr(VI), and salicylic acid, respectively.					
36563820	4	20	theme	mechanical	696:705	arg1	properties					707:716	the mechanical properties	692:716	the mechanical properties	692:716	Incorporating starch and gelatin as the backbone of these composite films can significantly improve the mechanical properties, color, and potential applications in real-time monitoring.					
36563820	6	21	from	0.001-0.05 mol/L	1116:1131	arg1	10 mL					1136:1140	10 mL	1136:1140	10 mL of milk with the LODs of 0.1	1136:1169	These preservatives ranged from 0.1 to 200.0 mg/L, 1.0-100.0 mg/L, 0.001-0.05 mol/L in 10 mL of milk with the LODs of 0.1, 1.0 mg/L, and 0.001 mmol/L, for H2O2, Cr(VI), and salicylic acid, respectively.					
36563820	1	22	theme	Milk	154:157	arg1	analysis					172:179	Milk adulteration analysis	154:179	Milk adulteration analysis at different stages of the production chain, which profiteers widely use to mask milk quality parameters,	154:285	Milk adulteration analysis at different stages of the production chain, which profiteers widely use to mask milk quality parameters, is problematic.					
36563820	2	23	theme	out-of-lab	404:413	arg1	setting					415:421	an out-of-lab setting	401:421	an out-of-lab setting	401:421	We prepared selective intelligent thin bio-films and a dedicated app to test milk adulteration in an out-of-lab setting.					
36563820	7	24	theme	milk	1320:1323	arg1	freshness/spoilage					1294:1311	the freshness/spoilage	1290:1311	the freshness/spoilage of the milk	1290:1323	The pH monitoring film also indicates the freshness/spoilage of the milk.					
36563820	5	25	theme	quantitative	982:993	arg1	information					995:1005	qualitative and quantitative information	966:1005	qualitative and quantitative information about the presence of some preservatives	966:1046	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	8	26	theme	use	1351:1353	arg1	ease					1343:1346	ease	1343:1346	ease of use	1343:1353	The portability, ease of use, and low cost of testing coupled with the app, make it an attractive alternative to lab-based analysis.					
36563820	8	26	theme	use	1351:1353	arg1	portability					1330:1340	The portability	1326:1340	The portability	1326:1340	The portability, ease of use, and low cost of testing coupled with the app, make it an attractive alternative to lab-based analysis.					
36563820	8	26	theme	use	1351:1353	arg1	cost					1364:1367	low cost	1360:1367	low cost of testing coupled with the app	1360:1399	The portability, ease of use, and low cost of testing coupled with the app, make it an attractive alternative to lab-based analysis.					
36563820	1	27	theme	adulteration	159:170	arg1	analysis					172:179	Milk adulteration analysis	154:179	Milk adulteration analysis at different stages of the production chain, which profiteers widely use to mask milk quality parameters,	154:285	Milk adulteration analysis at different stages of the production chain, which profiteers widely use to mask milk quality parameters, is problematic.					
36563820	5	28	theme	5 min	784:788	arg1	floating					790:797	5 min floating	784:797	5 min floating of the card on the surface of the milk	784:836	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	6	29	theme	salicylic	1222:1230	arg1	acid					1232:1235	salicylic acid	1222:1235	salicylic acid	1222:1235	These preservatives ranged from 0.1 to 200.0 mg/L, 1.0-100.0 mg/L, 0.001-0.05 mol/L in 10 mL of milk with the LODs of 0.1, 1.0 mg/L, and 0.001 mmol/L, for H2O2, Cr(VI), and salicylic acid, respectively.					
36563820	3	30	theme	color-changeable	505:520	arg1	reagent					445:451	each reagent	440:451	each reagent	440:451	In this regard, each reagent is immobilized on a piece of starch/gelatin film as color-changeable polymeric support, which is then pasted onto a transparent test card.					
36563820	3	30	theme	color-changeable	505:520	arg1	support					532:538	color-changeable polymeric support	505:538	color-changeable polymeric support	505:538	In this regard, each reagent is immobilized on a piece of starch/gelatin film as color-changeable polymeric support, which is then pasted onto a transparent test card.					
36563820	7	31	theme	monitoring	1259:1268	arg1	film					1270:1273	The pH monitoring film	1252:1273	The pH monitoring film	1252:1273	The pH monitoring film also indicates the freshness/spoilage of the milk.					
36563820	5	32	dep	QR	930:931	arg1	codes					933:937	codes	933:937	codes	933:937	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	0	33	theme	film	92:95	arg1	film					92:95	starch/gelatin intelligent film	65:95	starch/gelatin intelligent film	65:95	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	0	33	theme	film	92:95	arg1	bio-composite					48:60	a bio-composite	46:60	a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis	46:151	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	3	34	theme	polymeric	522:530	arg1	reagent					445:451	each reagent	440:451	each reagent	440:451	In this regard, each reagent is immobilized on a piece of starch/gelatin film as color-changeable polymeric support, which is then pasted onto a transparent test card.					
36563820	3	34	theme	polymeric	522:530	arg1	support					532:538	color-changeable polymeric support	505:538	color-changeable polymeric support	505:538	In this regard, each reagent is immobilized on a piece of starch/gelatin film as color-changeable polymeric support, which is then pasted onto a transparent test card.					
36563820	2	35	theme	milk	380:383	arg1	adulteration					385:396	milk adulteration	380:396	milk adulteration in an out-of-lab setting	380:421	We prepared selective intelligent thin bio-films and a dedicated app to test milk adulteration in an out-of-lab setting.					
36563820	0	36	theme	lab	20:22	arg1	card					2:5	A card	0:5	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.	0:152	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	1	37	theme	milk	262:265	arg1	parameters					275:284	milk quality parameters	262:284	milk quality parameters	262:284	Milk adulteration analysis at different stages of the production chain, which profiteers widely use to mask milk quality parameters, is problematic.					
36563820	0	38	theme	quality	106:112	arg1	test					114:117	milk quality test	101:117	milk quality test followed by colorimetric analysis	101:151	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	5	39	theme	card	806:809	arg1	floating					790:797	5 min floating	784:797	5 min floating of the card on the surface of the milk	784:836	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	6	40	theme	0.1	1167:1169	arg1	LODs					1159:1162	the LODs	1155:1162	the LODs of 0.1	1155:1169	These preservatives ranged from 0.1 to 200.0 mg/L, 1.0-100.0 mg/L, 0.001-0.05 mol/L in 10 mL of milk with the LODs of 0.1, 1.0 mg/L, and 0.001 mmol/L, for H2O2, Cr(VI), and salicylic acid, respectively.					
36563820	6	41	from	200.0 mg/L	1088:1097	arg1	10 mL					1136:1140	10 mL	1136:1140	10 mL of milk with the LODs of 0.1	1136:1169	These preservatives ranged from 0.1 to 200.0 mg/L, 1.0-100.0 mg/L, 0.001-0.05 mol/L in 10 mL of milk with the LODs of 0.1, 1.0 mg/L, and 0.001 mmol/L, for H2O2, Cr(VI), and salicylic acid, respectively.					
36563820	1	42	theme	different	184:192	arg1	stages					194:199	different stages	184:199	different stages of the production chain, which profiteers widely use to mask milk quality parameters,	184:285	Milk adulteration analysis at different stages of the production chain, which profiteers widely use to mask milk quality parameters, is problematic.					
36563820	1	43	theme	quality	267:273	arg1	parameters					275:284	milk quality parameters	262:284	milk quality parameters	262:284	Milk adulteration analysis at different stages of the production chain, which profiteers widely use to mask milk quality parameters, is problematic.					
36563820	0	44	theme	milk	101:104	arg1	test					114:117	milk quality test	101:117	milk quality test followed by colorimetric analysis	101:151	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	0	45	from	embedded	34:41	arg1	film					92:95	starch/gelatin intelligent film	65:95	starch/gelatin intelligent film	65:95	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	0	45	from	embedded	34:41	arg1	bio-composite					48:60	a bio-composite	46:60	a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis	46:151	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	5	46	used	used	902:905	arg2	software					853:860	our developed software	839:860	our developed software as a Progressive Web Application	839:893	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	8	47	theme	attractive	1413:1422	arg1	alternative					1424:1434	an attractive alternative	1410:1434	it an attractive alternative to lab-based analysis	1407:1456	The portability, ease of use, and low cost of testing coupled with the app, make it an attractive alternative to lab-based analysis.					
36563820	5	48	theme	samples	946:952	arg1	QR					930:931	the QR codes	926:937	the QR codes of the samples	926:952	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	1	49	from	stages	194:199	arg1	analysis					172:179	Milk adulteration analysis	154:179	Milk adulteration analysis at different stages of the production chain, which profiteers widely use to mask milk quality parameters,	154:285	Milk adulteration analysis at different stages of the production chain, which profiteers widely use to mask milk quality parameters, is problematic.					
36563820	8	50	theme	lab-based	1439:1447	arg1	analysis					1449:1456	lab-based analysis	1439:1456	lab-based analysis	1439:1456	The portability, ease of use, and low cost of testing coupled with the app, make it an attractive alternative to lab-based analysis.					
36563820	1	51	dep	profiteers	232:241	arg1	use					250:252	use	250:252	profiteers widely use to mask milk quality parameters	232:284	Milk adulteration analysis at different stages of the production chain, which profiteers widely use to mask milk quality parameters, is problematic.					
36563820	7	52	theme	pH	1256:1257	arg1	film					1270:1273	The pH monitoring film	1252:1273	The pH monitoring film	1252:1273	The pH monitoring film also indicates the freshness/spoilage of the milk.					
36563820	4	53	theme	films	660:664	arg1	gelatin					617:623	gelatin	617:623	gelatin	617:623	Incorporating starch and gelatin as the backbone of these composite films can significantly improve the mechanical properties, color, and potential applications in real-time monitoring.					
36563820	4	53	theme	films	660:664	arg1	backbone					632:639	the backbone	628:639	the backbone of these composite films	628:664	Incorporating starch and gelatin as the backbone of these composite films can significantly improve the mechanical properties, color, and potential applications in real-time monitoring.					
36563820	4	53	theme	films	660:664	arg1	starch					606:611	starch	606:611	starch	606:611	Incorporating starch and gelatin as the backbone of these composite films can significantly improve the mechanical properties, color, and potential applications in real-time monitoring.					
36563820	0	54	theme	colorimetric	131:142	arg1	analysis					144:151	colorimetric analysis	131:151	colorimetric analysis	131:151	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	5	55	theme	qualitative	966:976	arg1	information					995:1005	qualitative and quantitative information	966:1005	qualitative and quantitative information about the presence of some preservatives	966:1046	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	3	56	theme	transparent	569:579	arg1	card					586:589	a transparent test card	567:589	a transparent test card	567:589	In this regard, each reagent is immobilized on a piece of starch/gelatin film as color-changeable polymeric support, which is then pasted onto a transparent test card.					
36563820	5	57	theme	developed	843:851	arg1	software					853:860	our developed software	839:860	our developed software as a Progressive Web Application	839:893	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	3	58	theme	test	581:584	arg1	card					586:589	a transparent test card	567:589	a transparent test card	567:589	In this regard, each reagent is immobilized on a piece of starch/gelatin film as color-changeable polymeric support, which is then pasted onto a transparent test card.					
36563820	5	59	theme	Progressive	867:877	arg1	Application					883:893	a Progressive Web Application	865:893	a Progressive Web Application	865:893	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	6	60	from	0.001 mmol/L	1186:1197	arg1	10 mL					1136:1140	10 mL	1136:1140	10 mL of milk with the LODs of 0.1	1136:1169	These preservatives ranged from 0.1 to 200.0 mg/L, 1.0-100.0 mg/L, 0.001-0.05 mol/L in 10 mL of milk with the LODs of 0.1, 1.0 mg/L, and 0.001 mmol/L, for H2O2, Cr(VI), and salicylic acid, respectively.					
36563820	0	61	theme	embedded	34:41	arg1	ligand					27:32	A ligand	25:32	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.	0:152	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	0	62	from	bio-composite	48:60	arg1	embedded					34:41	embedded	34:41	embedded	34:41	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	0	63	dep	card	2:5	arg1	ligand					27:32	A ligand	25:32	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.	0:152	A card instead of a lab: A ligand embedded in a bio-composite of starch/gelatin intelligent film for milk quality test followed by colorimetric analysis.					
36563820	5	64	theme	milk	833:836	arg1	surface					818:824	the surface	814:824	the surface of the milk	814:836	After 5 min floating of the card on the surface of the milk, our developed software as a Progressive Web Application can be used to locate and read the QR codes of the samples to estimate qualitative and quantitative information about the presence of some preservatives.					
36563820	1	65	theme	production	208:217	arg1	chain					219:223	the production chain	204:223	the production chain	204:223	Milk adulteration analysis at different stages of the production chain, which profiteers widely use to mask milk quality parameters, is problematic.					
36563820	4	66	theme	real-time	756:764	arg1	monitoring					766:775	real-time monitoring	756:775	real-time monitoring	756:775	Incorporating starch and gelatin as the backbone of these composite films can significantly improve the mechanical properties, color, and potential applications in real-time monitoring.					
36563820	2	67	from	adulteration	385:396	arg1	setting					415:421	an out-of-lab setting	401:421	an out-of-lab setting	401:421	We prepared selective intelligent thin bio-films and a dedicated app to test milk adulteration in an out-of-lab setting.					
36563820	2	68	theme	thin	337:340	arg1	bio-films					342:350	selective intelligent thin bio-films	315:350	selective intelligent thin bio-films	315:350	We prepared selective intelligent thin bio-films and a dedicated app to test milk adulteration in an out-of-lab setting.					
36563820	1	69	theme	chain	219:223	arg1	stages					194:199	different stages	184:199	different stages of the production chain, which profiteers widely use to mask milk quality parameters,	184:285	Milk adulteration analysis at different stages of the production chain, which profiteers widely use to mask milk quality parameters, is problematic.					
35364200	0	0	theme	adsorptive	100:109	arg1	removal					111:117	adsorptive removal	100:117	adsorptive removal of hexavalent chromium ions	100:145	Novel reusable amine-functionalized cellulose acetate beads impregnated aminated graphene oxide for adsorptive removal of hexavalent chromium ions.					
35364200	9	1	theme	CA-NH2	1340:1345	arg1	surface					1347:1353	GO-NH2@CA-NH2 surface	1333:1353	GO-NH2@CA-NH2 surface	1333:1353	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface occurred via the electrostatic interactions, reduction process and coordinate-covalent bonds.					
35364200	2	2	theme	cellulose	354:362	arg1	CA					379:380	CA	379:380	CA	379:380	Herein, aminated graphene oxide (GO-NH2) was firstly synthetized and incorporated into cellulose acetate beads (CA) which were followed by surface amine-functionalization process.					
35364200	2	2	theme	cellulose	354:362	arg1	beads					372:376	cellulose acetate beads	354:376	cellulose acetate beads (CA) which were followed by surface amine-functionalization process	354:444	Herein, aminated graphene oxide (GO-NH2) was firstly synthetized and incorporated into cellulose acetate beads (CA) which were followed by surface amine-functionalization process.					
35364200	10	3	theme	@	1502:1502	arg1	beads					1510:1514	the fabricated GO-NH2@CA-NH2 beads	1481:1514	the fabricated GO-NH2@CA-NH2 beads	1481:1514	These findings hypothesize that the fabricated GO-NH2@CA-NH2 beads could be act as easy-separable and reusable adsorbent for efficient adsorption of Cr6+ ions.					
35364200	6	4	theme	adsorption	965:974	arg1	capacity					976:983	a supreme adsorption capacity	955:983	a supreme adsorption capacity of 410.21 mg/g at pH 2	955:1006	Furthermore, the adsorption process agreed with Freundlich isotherm model with a supreme adsorption capacity of 410.21 mg/g at pH 2, while data followed the pseudo-second-order kinetic model.					
35364200	10	5	dep	easy-separable	1532:1545	arg1	adsorbent					1560:1568	adsorbent	1560:1568	adsorbent	1560:1568	These findings hypothesize that the fabricated GO-NH2@CA-NH2 beads could be act as easy-separable and reusable adsorbent for efficient adsorption of Cr6+ ions.					
35364200	8	6	theme	better	1209:1214	arg1	characteristics					1227:1241	better adsorption characteristics	1209:1241	better adsorption characteristics	1209:1241	The composite beads retained better adsorption characteristics for seven sequential cycles with ease of separation.					
35364200	1	7	theme	ions	261:264	arg1	removal					223:229	the adsorptive removal	208:229	the adsorptive removal of hexavalent chromium (Cr6+) ions	208:264	In this study, a multi-featured adsorbent was developed for the adsorptive removal of hexavalent chromium (Cr6+) ions.					
35364200	10	8	theme	ions	1603:1606	arg1	adsorption					1584:1593	efficient adsorption	1574:1593	efficient adsorption of Cr6+ ions	1574:1606	These findings hypothesize that the fabricated GO-NH2@CA-NH2 beads could be act as easy-separable and reusable adsorbent for efficient adsorption of Cr6+ ions.					
35364200	9	9	theme	proposed	1300:1307	arg1	adsorption					1309:1318	The proposed adsorption	1296:1318	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface	1296:1353	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface occurred via the electrostatic interactions, reduction process and coordinate-covalent bonds.					
35364200	7	10	theme	thermodynamic	1077:1089	arg1	studies					1091:1097	thermodynamic studies	1077:1097	thermodynamic studies	1077:1097	Besides, thermodynamic studies denoted that the adsorption process was endothermic, randomness and spontaneous.					
35364200	10	11	theme	Cr6+	1598:1601	arg1	ions					1603:1606	Cr6+ ions	1598:1606	Cr6+ ions	1598:1606	These findings hypothesize that the fabricated GO-NH2@CA-NH2 beads could be act as easy-separable and reusable adsorbent for efficient adsorption of Cr6+ ions.					
35364200	2	12	theme	graphene	284:291	arg1	GO-NH2					300:305	GO-NH2	300:305	GO-NH2	300:305	Herein, aminated graphene oxide (GO-NH2) was firstly synthetized and incorporated into cellulose acetate beads (CA) which were followed by surface amine-functionalization process.					
35364200	2	12	theme	graphene	284:291	arg1	oxide					293:297	aminated graphene oxide	275:297	aminated graphene oxide (GO-NH2)	275:306	Herein, aminated graphene oxide (GO-NH2) was firstly synthetized and incorporated into cellulose acetate beads (CA) which were followed by surface amine-functionalization process.					
35364200	5	13	theme	GO-NH2	827:832	arg1	ratio					834:838	GO-NH2 ratio	827:838	GO-NH2 ratio in the beads matrix	827:858	Therefore, the adsorption capacity was boosted with increasing GO-NH2 ratio in the beads matrix from 10 to 25%.					
35364200	0	14	theme	chromium	133:140	arg1	ions					142:145	hexavalent chromium ions	122:145	hexavalent chromium ions	122:145	Novel reusable amine-functionalized cellulose acetate beads impregnated aminated graphene oxide for adsorptive removal of hexavalent chromium ions.					
35364200	8	15	theme	sequential	1253:1262	arg1	cycles					1264:1269	seven sequential cycles	1247:1269	seven sequential cycles with ease of separation	1247:1293	The composite beads retained better adsorption characteristics for seven sequential cycles with ease of separation.					
35364200	5	16	theme	adsorption	779:788	arg1	capacity					790:797	the adsorption capacity	775:797	the adsorption capacity	775:797	Therefore, the adsorption capacity was boosted with increasing GO-NH2 ratio in the beads matrix from 10 to 25%.					
35364200	2	17	theme	aminated	275:282	arg1	GO-NH2					300:305	GO-NH2	300:305	GO-NH2	300:305	Herein, aminated graphene oxide (GO-NH2) was firstly synthetized and incorporated into cellulose acetate beads (CA) which were followed by surface amine-functionalization process.					
35364200	2	17	theme	aminated	275:282	arg1	oxide					293:297	aminated graphene oxide	275:297	aminated graphene oxide (GO-NH2)	275:306	Herein, aminated graphene oxide (GO-NH2) was firstly synthetized and incorporated into cellulose acetate beads (CA) which were followed by surface amine-functionalization process.					
35364200	4	18	from	enhancement	636:646	arg1	performance					666:676	the adsorption performance	651:676	the adsorption performance	651:676	An enhancement in the adsorption performance was attained, while the adsorption equilibrium was closely gotten within only 60 min.					
35364200	6	19	theme	kinetic	1053:1059	arg1	model					1061:1065	the pseudo-second-order kinetic model	1029:1065	the pseudo-second-order kinetic model	1029:1065	Furthermore, the adsorption process agreed with Freundlich isotherm model with a supreme adsorption capacity of 410.21 mg/g at pH 2, while data followed the pseudo-second-order kinetic model.					
35364200	0	20	theme	hexavalent	122:131	arg1	ions					142:145	hexavalent chromium ions	122:145	hexavalent chromium ions	122:145	Novel reusable amine-functionalized cellulose acetate beads impregnated aminated graphene oxide for adsorptive removal of hexavalent chromium ions.					
35364200	9	21	theme	Cr6+	1323:1326	arg1	adsorption					1309:1318	The proposed adsorption	1296:1318	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface	1296:1353	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface occurred via the electrostatic interactions, reduction process and coordinate-covalent bonds.					
35364200	10	22	theme	GO-NH2	1496:1501	arg1	beads					1510:1514	the fabricated GO-NH2@CA-NH2 beads	1481:1514	the fabricated GO-NH2@CA-NH2 beads	1481:1514	These findings hypothesize that the fabricated GO-NH2@CA-NH2 beads could be act as easy-separable and reusable adsorbent for efficient adsorption of Cr6+ ions.					
35364200	10	23	theme	CA-NH2	1503:1508	arg1	beads					1510:1514	the fabricated GO-NH2@CA-NH2 beads	1481:1514	the fabricated GO-NH2@CA-NH2 beads	1481:1514	These findings hypothesize that the fabricated GO-NH2@CA-NH2 beads could be act as easy-separable and reusable adsorbent for efficient adsorption of Cr6+ ions.					
35364200	4	24	theme	only	751:754	arg1	60 min					756:761	only 60 min	751:761	only 60 min	751:761	An enhancement in the adsorption performance was attained, while the adsorption equilibrium was closely gotten within only 60 min.					
35364200	6	25	theme	pseudo-second-order	1033:1051	arg1	model					1061:1065	the pseudo-second-order kinetic model	1029:1065	the pseudo-second-order kinetic model	1029:1065	Furthermore, the adsorption process agreed with Freundlich isotherm model with a supreme adsorption capacity of 410.21 mg/g at pH 2, while data followed the pseudo-second-order kinetic model.					
35364200	0	26	theme	reusable	6:13	arg1	beads					54:58	Novel reusable amine-functionalized cellulose acetate beads	0:58	Novel reusable amine-functionalized cellulose acetate beads	0:58	Novel reusable amine-functionalized cellulose acetate beads impregnated aminated graphene oxide for adsorptive removal of hexavalent chromium ions.					
35364200	8	27	with	cycles	1264:1269	arg1	ease					1276:1279	ease	1276:1279	ease of separation	1276:1293	The composite beads retained better adsorption characteristics for seven sequential cycles with ease of separation.					
35364200	10	28	theme	fabricated	1485:1494	arg1	beads					1510:1514	the fabricated GO-NH2@CA-NH2 beads	1481:1514	the fabricated GO-NH2@CA-NH2 beads	1481:1514	These findings hypothesize that the fabricated GO-NH2@CA-NH2 beads could be act as easy-separable and reusable adsorbent for efficient adsorption of Cr6+ ions.					
35364200	6	29	from	pH 2	1003:1006	arg1	capacity					976:983	a supreme adsorption capacity	955:983	a supreme adsorption capacity of 410.21 mg/g at pH 2	955:1006	Furthermore, the adsorption process agreed with Freundlich isotherm model with a supreme adsorption capacity of 410.21 mg/g at pH 2, while data followed the pseudo-second-order kinetic model.					
35364200	5	30	theme	beads	847:851	arg1	matrix					853:858	the beads matrix	843:858	the beads matrix	843:858	Therefore, the adsorption capacity was boosted with increasing GO-NH2 ratio in the beads matrix from 10 to 25%.					
35364200	0	31	theme	Novel	0:4	arg1	beads					54:58	Novel reusable amine-functionalized cellulose acetate beads	0:58	Novel reusable amine-functionalized cellulose acetate beads	0:58	Novel reusable amine-functionalized cellulose acetate beads impregnated aminated graphene oxide for adsorptive removal of hexavalent chromium ions.					
35364200	9	32	theme	GO-NH2	1333:1338	arg1	surface					1347:1353	GO-NH2@CA-NH2 surface	1333:1353	GO-NH2@CA-NH2 surface	1333:1353	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface occurred via the electrostatic interactions, reduction process and coordinate-covalent bonds.					
35364200	4	33	theme	adsorption	702:711	arg1	equilibrium					713:723	the adsorption equilibrium	698:723	the adsorption equilibrium	698:723	An enhancement in the adsorption performance was attained, while the adsorption equilibrium was closely gotten within only 60 min.					
35364200	4	33	theme	adsorption	702:711	arg1	gotten					737:742	gotten	737:742	gotten	737:742	An enhancement in the adsorption performance was attained, while the adsorption equilibrium was closely gotten within only 60 min.					
35364200	0	34	theme	cellulose	36:44	arg1	beads					54:58	Novel reusable amine-functionalized cellulose acetate beads	0:58	Novel reusable amine-functionalized cellulose acetate beads	0:58	Novel reusable amine-functionalized cellulose acetate beads impregnated aminated graphene oxide for adsorptive removal of hexavalent chromium ions.					
35364200	6	35	theme	isotherm	935:942	arg1	model					944:948	Freundlich isotherm model	924:948	Freundlich isotherm model	924:948	Furthermore, the adsorption process agreed with Freundlich isotherm model with a supreme adsorption capacity of 410.21 mg/g at pH 2, while data followed the pseudo-second-order kinetic model.					
35364200	2	36	theme	surface	406:412	arg1	process					438:444	surface amine-functionalization process	406:444	surface amine-functionalization process	406:444	Herein, aminated graphene oxide (GO-NH2) was firstly synthetized and incorporated into cellulose acetate beads (CA) which were followed by surface amine-functionalization process.					
35364200	2	37	theme	amine-functionalization	414:436	arg1	process					438:444	surface amine-functionalization process	406:444	surface amine-functionalization process	406:444	Herein, aminated graphene oxide (GO-NH2) was firstly synthetized and incorporated into cellulose acetate beads (CA) which were followed by surface amine-functionalization process.					
35364200	6	38	theme	Freundlich	924:933	arg1	model					944:948	Freundlich isotherm model	924:948	Freundlich isotherm model	924:948	Furthermore, the adsorption process agreed with Freundlich isotherm model with a supreme adsorption capacity of 410.21 mg/g at pH 2, while data followed the pseudo-second-order kinetic model.					
35364200	0	39	theme	ions	142:145	arg1	removal					111:117	adsorptive removal	100:117	adsorptive removal of hexavalent chromium ions	100:145	Novel reusable amine-functionalized cellulose acetate beads impregnated aminated graphene oxide for adsorptive removal of hexavalent chromium ions.					
35364200	3	40	theme	composite	616:624	arg1	beads					626:630	GO-NH2@CA-NH2 composite beads	602:630	GO-NH2@CA-NH2 composite beads	602:630	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	3	41	theme	zeta	533:536	arg1	potential					538:546	zeta potential	533:546	zeta potential	533:546	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	1	42	theme	adsorptive	212:221	arg1	removal					223:229	the adsorptive removal	208:229	the adsorptive removal of hexavalent chromium (Cr6+) ions	208:264	In this study, a multi-featured adsorbent was developed for the adsorptive removal of hexavalent chromium (Cr6+) ions.					
35364200	3	43	theme	beads	626:630	arg1	fabrication					587:597	the successful fabrication	572:597	the successful fabrication of GO-NH2@CA-NH2 composite beads	572:630	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	3	44	theme	characterization	454:469	arg1	TGA					510:512	TGA	510:512	TGA	510:512	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	3	44	theme	characterization	454:469	arg1	potential					538:546	zeta potential	533:546	zeta potential	533:546	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	3	44	theme	characterization	454:469	arg1	spectroscopy					491:502	FT-IR spectroscopy	485:502	FT-IR spectroscopy	485:502	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	3	44	theme	characterization	454:469	arg1	SEM					505:507	SEM	505:507	SEM	505:507	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	3	44	theme	characterization	454:469	arg1	BET					520:522	BET	520:522	BET	520:522	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	3	44	theme	characterization	454:469	arg1	tools					471:475	characterization tools	454:475	characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential	454:546	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	3	44	theme	characterization	454:469	arg1	XPS					525:527	XPS	525:527	XPS	525:527	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	0	45	theme	acetate	46:52	arg1	beads					54:58	Novel reusable amine-functionalized cellulose acetate beads	0:58	Novel reusable amine-functionalized cellulose acetate beads	0:58	Novel reusable amine-functionalized cellulose acetate beads impregnated aminated graphene oxide for adsorptive removal of hexavalent chromium ions.					
35364200	6	46	theme	supreme	957:963	arg1	capacity					976:983	a supreme adsorption capacity	955:983	a supreme adsorption capacity of 410.21 mg/g at pH 2	955:1006	Furthermore, the adsorption process agreed with Freundlich isotherm model with a supreme adsorption capacity of 410.21 mg/g at pH 2, while data followed the pseudo-second-order kinetic model.					
35364200	3	47	theme	@	608:608	arg1	beads					626:630	GO-NH2@CA-NH2 composite beads	602:630	GO-NH2@CA-NH2 composite beads	602:630	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	8	48	theme	composite	1184:1192	arg1	beads					1194:1198	The composite beads	1180:1198	The composite beads	1180:1198	The composite beads retained better adsorption characteristics for seven sequential cycles with ease of separation.					
35364200	0	49	theme	aminated	72:79	arg1	oxide					90:94	aminated graphene oxide	72:94	aminated graphene oxide	72:94	Novel reusable amine-functionalized cellulose acetate beads impregnated aminated graphene oxide for adsorptive removal of hexavalent chromium ions.					
35364200	3	50	theme	CA-NH2	609:614	arg1	beads					626:630	GO-NH2@CA-NH2 composite beads	602:630	GO-NH2@CA-NH2 composite beads	602:630	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	5	51	from	ratio	834:838	arg1	matrix					853:858	the beads matrix	843:858	the beads matrix	843:858	Therefore, the adsorption capacity was boosted with increasing GO-NH2 ratio in the beads matrix from 10 to 25%.					
35364200	7	52	theme	adsorption	1116:1125	arg1	process					1127:1133	the adsorption process	1112:1133	the adsorption process	1112:1133	Besides, thermodynamic studies denoted that the adsorption process was endothermic, randomness and spontaneous.					
35364200	7	52	theme	adsorption	1116:1125	arg1	endothermic					1139:1149	endothermic	1139:1149	endothermic	1139:1149	Besides, thermodynamic studies denoted that the adsorption process was endothermic, randomness and spontaneous.					
35364200	6	53	theme	adsorption	893:902	arg1	process					904:910	the adsorption process	889:910	the adsorption process	889:910	Furthermore, the adsorption process agreed with Freundlich isotherm model with a supreme adsorption capacity of 410.21 mg/g at pH 2, while data followed the pseudo-second-order kinetic model.					
35364200	3	54	dep	Varies	447:452	arg1	employed					553:560	employed	553:560	were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads	548:630	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	8	55	theme	separation	1284:1293	arg1	ease					1276:1279	ease	1276:1279	ease of separation	1276:1293	The composite beads retained better adsorption characteristics for seven sequential cycles with ease of separation.					
35364200	9	56	theme	electrostatic	1372:1384	arg1	interactions					1386:1397	the electrostatic interactions	1368:1397	the electrostatic interactions	1368:1397	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface occurred via the electrostatic interactions, reduction process and coordinate-covalent bonds.					
35364200	9	56	theme	electrostatic	1372:1384	arg1	process					1410:1416	reduction process	1400:1416	reduction process	1400:1416	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface occurred via the electrostatic interactions, reduction process and coordinate-covalent bonds.					
35364200	9	56	theme	electrostatic	1372:1384	arg1	bonds					1442:1446	coordinate-covalent bonds	1422:1446	coordinate-covalent bonds	1422:1446	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface occurred via the electrostatic interactions, reduction process and coordinate-covalent bonds.					
35364200	6	57	theme	410.21 mg/g	988:998	arg1	capacity					976:983	a supreme adsorption capacity	955:983	a supreme adsorption capacity of 410.21 mg/g at pH 2	955:1006	Furthermore, the adsorption process agreed with Freundlich isotherm model with a supreme adsorption capacity of 410.21 mg/g at pH 2, while data followed the pseudo-second-order kinetic model.					
35364200	3	58	theme	FT-IR	485:489	arg1	spectroscopy					491:502	FT-IR spectroscopy	485:502	FT-IR spectroscopy	485:502	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	1	59	theme	hexavalent	234:243	arg1	ions					261:264	hexavalent chromium (Cr6+) ions	234:264	hexavalent chromium (Cr6+) ions	234:264	In this study, a multi-featured adsorbent was developed for the adsorptive removal of hexavalent chromium (Cr6+) ions.					
35364200	9	60	theme	coordinate-covalent	1422:1440	arg1	interactions					1386:1397	the electrostatic interactions	1368:1397	the electrostatic interactions	1368:1397	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface occurred via the electrostatic interactions, reduction process and coordinate-covalent bonds.					
35364200	9	60	theme	coordinate-covalent	1422:1440	arg1	bonds					1442:1446	coordinate-covalent bonds	1422:1446	coordinate-covalent bonds	1422:1446	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface occurred via the electrostatic interactions, reduction process and coordinate-covalent bonds.					
35364200	3	61	theme	GO-NH2	602:607	arg1	beads					626:630	GO-NH2@CA-NH2 composite beads	602:630	GO-NH2@CA-NH2 composite beads	602:630	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	9	62	theme	@	1339:1339	arg1	surface					1347:1353	GO-NH2@CA-NH2 surface	1333:1353	GO-NH2@CA-NH2 surface	1333:1353	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface occurred via the electrostatic interactions, reduction process and coordinate-covalent bonds.					
35364200	5	63	dep	25	871:872	arg1	to					868:869	to	868:869	to	868:869	Therefore, the adsorption capacity was boosted with increasing GO-NH2 ratio in the beads matrix from 10 to 25%.					
35364200	8	64	theme	adsorption	1216:1225	arg1	characteristics					1227:1241	better adsorption characteristics	1209:1241	better adsorption characteristics	1209:1241	The composite beads retained better adsorption characteristics for seven sequential cycles with ease of separation.					
35364200	1	65	theme	chromium	245:252	arg1	ions					261:264	hexavalent chromium (Cr6+) ions	234:264	hexavalent chromium (Cr6+) ions	234:264	In this study, a multi-featured adsorbent was developed for the adsorptive removal of hexavalent chromium (Cr6+) ions.					
35364200	10	66	theme	efficient	1574:1582	arg1	adsorption					1584:1593	efficient adsorption	1574:1593	efficient adsorption of Cr6+ ions	1574:1606	These findings hypothesize that the fabricated GO-NH2@CA-NH2 beads could be act as easy-separable and reusable adsorbent for efficient adsorption of Cr6+ ions.					
35364200	3	67	theme	successful	576:585	arg1	fabrication					587:597	the successful fabrication	572:597	the successful fabrication of GO-NH2@CA-NH2 composite beads	572:630	Varies characterization tools such as FT-IR spectroscopy, SEM, TGA, XRD, BET, XPS and zeta potential were employed to ensure the successful fabrication of GO-NH2@CA-NH2 composite beads.					
35364200	4	68	theme	adsorption	655:664	arg1	performance					666:676	the adsorption performance	651:676	the adsorption performance	651:676	An enhancement in the adsorption performance was attained, while the adsorption equilibrium was closely gotten within only 60 min.					
35364200	9	69	theme	reduction	1400:1408	arg1	interactions					1386:1397	the electrostatic interactions	1368:1397	the electrostatic interactions	1368:1397	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface occurred via the electrostatic interactions, reduction process and coordinate-covalent bonds.					
35364200	9	69	theme	reduction	1400:1408	arg1	process					1410:1416	reduction process	1400:1416	reduction process	1400:1416	The proposed adsorption of Cr6+ onto GO-NH2@CA-NH2 surface occurred via the electrostatic interactions, reduction process and coordinate-covalent bonds.					
35364200	0	70	theme	graphene	81:88	arg1	oxide					90:94	aminated graphene oxide	72:94	aminated graphene oxide	72:94	Novel reusable amine-functionalized cellulose acetate beads impregnated aminated graphene oxide for adsorptive removal of hexavalent chromium ions.					
35364200	0	71	theme	amine-functionalized	15:34	arg1	beads					54:58	Novel reusable amine-functionalized cellulose acetate beads	0:58	Novel reusable amine-functionalized cellulose acetate beads	0:58	Novel reusable amine-functionalized cellulose acetate beads impregnated aminated graphene oxide for adsorptive removal of hexavalent chromium ions.					
35364200	2	72	theme	acetate	364:370	arg1	CA					379:380	CA	379:380	CA	379:380	Herein, aminated graphene oxide (GO-NH2) was firstly synthetized and incorporated into cellulose acetate beads (CA) which were followed by surface amine-functionalization process.					
35364200	2	72	theme	acetate	364:370	arg1	beads					372:376	cellulose acetate beads	354:376	cellulose acetate beads (CA) which were followed by surface amine-functionalization process	354:444	Herein, aminated graphene oxide (GO-NH2) was firstly synthetized and incorporated into cellulose acetate beads (CA) which were followed by surface amine-functionalization process.					
35364200	1	73	theme	Cr6+	255:258	arg1	ions					261:264	hexavalent chromium (Cr6+) ions	234:264	hexavalent chromium (Cr6+) ions	234:264	In this study, a multi-featured adsorbent was developed for the adsorptive removal of hexavalent chromium (Cr6+) ions.					
35296788	3	0	theme	Research	521:528	arg1	results					530:536	Research results	521:536	Research results presenting the impact of process parameters of microwave-assisted hydrotropic delignification	521:630	Research results presenting the impact of process parameters of microwave-assisted hydrotropic delignification confirm a high effectiveness of this method of pretreatment of lignocellulosic biomass.					
35296788	6	1	theme	hydrolysis	1441:1450	arg1	%					1469:1469	hydrolysis yield 6.63 ± 0.10%	1441:1469	hydrolysis yield 6.63 ± 0.10%	1441:1469	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	6	1	theme	hydrolysis	1441:1450	arg1	glucose/g					1430:1438	only 50.77 ± 0.75 mg glucose/g	1409:1438	only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%)	1409:1470	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	3	2	theme	process	563:569	arg1	parameters					571:580	process parameters	563:580	process parameters of microwave-assisted hydrotropic delignification	563:630	Research results presenting the impact of process parameters of microwave-assisted hydrotropic delignification confirm a high effectiveness of this method of pretreatment of lignocellulosic biomass.					
35296788	1	3	theme	method	119:124	arg1	use					110:112	The use	106:112	The use of a method of an effective delignification of lignocellulosic biomass	106:183	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	1	3	theme	method	119:124	arg1	stage					194:198	a key stage	188:198	a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol	188:317	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	6	4	theme	yield	1452:1456	arg1	%					1469:1469	hydrolysis yield 6.63 ± 0.10%	1441:1469	hydrolysis yield 6.63 ± 0.10%	1441:1469	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	6	4	theme	yield	1452:1456	arg1	glucose/g					1430:1438	only 50.77 ± 0.75 mg glucose/g	1409:1438	only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%)	1409:1470	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	8	5	theme	biomass	1921:1927	arg1	component					1929:1937	the main biomass component	1912:1937	the main biomass component	1912:1937	Due to a significant reduction of lignin and hemicellulose concentration in biomass, cellulose-which is susceptible to enzymatic hydrolysis and a source of carbon in biosynthesis processes-becomes the main biomass component.					
35296788	6	6	theme	50.77 ± 0.75 mg	1414:1428	arg1	%					1469:1469	hydrolysis yield 6.63 ± 0.10%	1441:1469	hydrolysis yield 6.63 ± 0.10%	1441:1469	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	6	6	theme	50.77 ± 0.75 mg	1414:1428	arg1	glucose/g					1430:1438	only 50.77 ± 0.75 mg glucose/g	1409:1438	only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%)	1409:1470	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	4	7	theme	observed	724:731	arg1	effects					733:739	The observed effects	720:739	The observed effects	720:739	The observed effects included changes in the composition of the biomass and an increased susceptibility of cellulose to the subsequent enzymatic hydrolysis.					
35296788	6	8	theme	hydrolysis	1236:1245	arg1	%					1265:1265	hydrolysis yield 46.76 ± 1.14%	1236:1265	hydrolysis yield 46.76 ± 1.14%	1236:1265	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	6	8	theme	hydrolysis	1236:1245	arg1	glucose/g					1225:1233	glucose: 463.27 ± 11.25 mg glucose/g	1198:1233	glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%)	1198:1266	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	3	9	theme	hydrotropic	604:614	arg1	delignification					616:630	microwave-assisted hydrotropic delignification	585:630	microwave-assisted hydrotropic delignification	585:630	Research results presenting the impact of process parameters of microwave-assisted hydrotropic delignification confirm a high effectiveness of this method of pretreatment of lignocellulosic biomass.					
35296788	8	10	theme	lignin	1749:1754	arg1	reduction					1736:1744	a significant reduction	1722:1744	a significant reduction of lignin and hemicellulose concentration in biomass	1722:1797	Due to a significant reduction of lignin and hemicellulose concentration in biomass, cellulose-which is susceptible to enzymatic hydrolysis and a source of carbon in biosynthesis processes-becomes the main biomass component.					
35296788	5	11	theme	hydrotrope	935:944	arg1	addition					923:930	an addition	920:930	an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min	920:983	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	8	12	theme	hemicellulose	1760:1772	arg1	concentration					1774:1786	hemicellulose concentration	1760:1786	hemicellulose concentration	1760:1786	Due to a significant reduction of lignin and hemicellulose concentration in biomass, cellulose-which is susceptible to enzymatic hydrolysis and a source of carbon in biosynthesis processes-becomes the main biomass component.					
35296788	5	13	theme	heating	898:904	arg1	use					881:883	The use	877:883	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min	877:983	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	5	14	theme	beech	1084:1088	arg1	chips					1090:1094	beech chips	1084:1094	beech chips	1084:1094	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	3	15	theme	high	642:645	arg1	effectiveness					647:659	a high effectiveness	640:659	a high effectiveness of this method of pretreatment of lignocellulosic biomass	640:717	Research results presenting the impact of process parameters of microwave-assisted hydrotropic delignification confirm a high effectiveness of this method of pretreatment of lignocellulosic biomass.					
35296788	7	16	theme	hemicellulose	1629:1641	arg1	removal					1618:1624	a complete removal	1607:1624	a complete removal	1607:1624	Microwave-assisted hydrotropic delignification in the optimum process conditions additionally allows a complete removal of hemicellulose from biomass, which improves the effectiveness of enzymatic hydrolysis.					
35296788	6	17	theme	463.27 ± 11.25 mg	1207:1223	arg1	%					1265:1265	hydrolysis yield 46.76 ± 1.14%	1236:1265	hydrolysis yield 46.76 ± 1.14%	1236:1265	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	6	17	theme	463.27 ± 11.25 mg	1207:1223	arg1	glucose/g					1225:1233	glucose: 463.27 ± 11.25 mg glucose/g	1198:1233	glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%)	1198:1266	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	6	18	theme	glucose	1198:1204	arg1	%					1265:1265	hydrolysis yield 46.76 ± 1.14%	1236:1265	hydrolysis yield 46.76 ± 1.14%	1236:1265	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	6	18	theme	glucose	1198:1204	arg1	glucose/g					1225:1233	glucose: 463.27 ± 11.25 mg glucose/g	1198:1233	glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%)	1198:1266	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	3	19	theme	method	669:674	arg1	effectiveness					647:659	a high effectiveness	640:659	a high effectiveness of this method of pretreatment of lignocellulosic biomass	640:717	Research results presenting the impact of process parameters of microwave-assisted hydrotropic delignification confirm a high effectiveness of this method of pretreatment of lignocellulosic biomass.					
35296788	5	20	theme	PSI	970:972	arg1	hydrotrope					935:944	hydrotrope	935:944	hydrotrope of 40% w/v NaCS and 117 PSI for 60 min	935:983	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	0	21	theme	microwave-assisted	61:78	arg1	pretreatment					92:103	microwave-assisted hydrotropic pretreatment	61:103	microwave-assisted hydrotropic pretreatment	61:103	Delignification efficiency of various types of biomass using microwave-assisted hydrotropic pretreatment.					
35296788	5	22	theme	40	949:950	arg1	%					951:951	%	951:951	%	951:951	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	5	23	theme	wheat	1114:1118	arg1	straw					1120:1124	wheat straw	1114:1124	wheat straw	1114:1124	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	1	24	theme	biomass	177:183	arg1	delignification					142:156	an effective delignification	129:156	an effective delignification of lignocellulosic biomass	129:183	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	7	25	theme	process	1568:1574	arg1	conditions					1576:1585	the optimum process conditions	1556:1585	the optimum process conditions	1556:1585	Microwave-assisted hydrotropic delignification in the optimum process conditions additionally allows a complete removal of hemicellulose from biomass, which improves the effectiveness of enzymatic hydrolysis.					
35296788	1	26	theme	effective	132:140	arg1	delignification					142:156	an effective delignification	129:156	an effective delignification of lignocellulosic biomass	129:183	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	2	27	theme	sodium	425:430	arg1	sulfonate					439:447	sodium cumene sulfonate	425:447	sodium cumene sulfonate (NaCS)	425:454	The study was aimed at evaluating the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS) for the delignification of pine and beech chips and wheat straw.					
35296788	2	27	theme	sodium	425:430	arg1	NaCS					450:453	NaCS	450:453	NaCS	450:453	The study was aimed at evaluating the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS) for the delignification of pine and beech chips and wheat straw.					
35296788	6	28	theme	hydrolysis	1338:1347	arg1	%					1367:1367	hydrolysis yield 35.13 ± 2.37%	1338:1367	hydrolysis yield 35.13 ± 2.37%	1338:1367	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	6	28	theme	hydrolysis	1338:1347	arg1	glucose/g					1327:1335	327.70 ± 22.15 mg glucose/g	1309:1335	327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%)	1309:1368	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	7	29	from	biomass	1648:1654	arg1	removal					1618:1624	a complete removal	1607:1624	a complete removal	1607:1624	Microwave-assisted hydrotropic delignification in the optimum process conditions additionally allows a complete removal of hemicellulose from biomass, which improves the effectiveness of enzymatic hydrolysis.					
35296788	5	30	theme	absolute	1012:1019	arg1	concentration					1021:1033	the absolute concentration	1008:1033	the absolute concentration of lignins	1008:1044	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	7	31	theme	enzymatic	1693:1701	arg1	hydrolysis					1703:1712	enzymatic hydrolysis	1693:1712	enzymatic hydrolysis	1693:1712	Microwave-assisted hydrotropic delignification in the optimum process conditions additionally allows a complete removal of hemicellulose from biomass, which improves the effectiveness of enzymatic hydrolysis.					
35296788	2	32	theme	pretreatment	406:417	arg1	effectiveness					358:370	the effectiveness	354:370	the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS)	354:454	The study was aimed at evaluating the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS) for the delignification of pine and beech chips and wheat straw.					
35296788	0	33	theme	Delignification	0:14	arg1	efficiency					16:25	Delignification efficiency	0:25	Delignification efficiency of various types of biomass	0:53	Delignification efficiency of various types of biomass using microwave-assisted hydrotropic pretreatment.					
35296788	4	34	theme	increased	799:807	arg1	susceptibility					809:822	an increased susceptibility	796:822	an increased susceptibility of cellulose to the subsequent enzymatic hydrolysis	796:874	The observed effects included changes in the composition of the biomass and an increased susceptibility of cellulose to the subsequent enzymatic hydrolysis.					
35296788	7	35	theme	hydrotropic	1525:1535	arg1	delignification					1537:1551	Microwave-assisted hydrotropic delignification	1506:1551	Microwave-assisted hydrotropic delignification in the optimum process conditions	1506:1585	Microwave-assisted hydrotropic delignification in the optimum process conditions additionally allows a complete removal of hemicellulose from biomass, which improves the effectiveness of enzymatic hydrolysis.					
35296788	2	36	theme	beech	492:496	arg1	chips					498:502	pine and beech chips	483:502	pine and beech chips	483:502	The study was aimed at evaluating the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS) for the delignification of pine and beech chips and wheat straw.					
35296788	3	37	theme	lignocellulosic	695:709	arg1	biomass					711:717	lignocellulosic biomass	695:717	lignocellulosic biomass	695:717	Research results presenting the impact of process parameters of microwave-assisted hydrotropic delignification confirm a high effectiveness of this method of pretreatment of lignocellulosic biomass.					
35296788	8	38	from	reduction	1736:1744	arg1	biomass					1791:1797	biomass	1791:1797	biomass	1791:1797	Due to a significant reduction of lignin and hemicellulose concentration in biomass, cellulose-which is susceptible to enzymatic hydrolysis and a source of carbon in biosynthesis processes-becomes the main biomass component.					
35296788	0	39	theme	types	38:42	arg1	efficiency					16:25	Delignification efficiency	0:25	Delignification efficiency of various types of biomass	0:53	Delignification efficiency of various types of biomass using microwave-assisted hydrotropic pretreatment.					
35296788	1	40	theme	ethanol	311:317	arg1	production					286:295	the production	282:295	the production of cellulosic ethanol	282:317	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	2	41	theme	pine	483:486	arg1	chips					498:502	pine and beech chips	483:502	pine and beech chips	483:502	The study was aimed at evaluating the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS) for the delignification of pine and beech chips and wheat straw.					
35296788	3	42	theme	pretreatment	679:690	arg1	method					669:674	this method	664:674	this method of pretreatment of lignocellulosic biomass	664:717	Research results presenting the impact of process parameters of microwave-assisted hydrotropic delignification confirm a high effectiveness of this method of pretreatment of lignocellulosic biomass.					
35296788	0	43	theme	biomass	47:53	arg1	types					38:42	various types	30:42	various types of biomass	30:53	Delignification efficiency of various types of biomass using microwave-assisted hydrotropic pretreatment.					
35296788	5	44	theme	pine	1059:1062	arg1	chips					1064:1068	pine chips	1059:1068	pine chips	1059:1068	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	4	45	theme	subsequent	844:853	arg1	hydrolysis					865:874	the subsequent enzymatic hydrolysis	840:874	the subsequent enzymatic hydrolysis	840:874	The observed effects included changes in the composition of the biomass and an increased susceptibility of cellulose to the subsequent enzymatic hydrolysis.					
35296788	6	46	theme	only	1409:1412	arg1	%					1469:1469	hydrolysis yield 6.63 ± 0.10%	1441:1469	hydrolysis yield 6.63 ± 0.10%	1441:1469	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	6	46	theme	only	1409:1412	arg1	glucose/g					1430:1438	only 50.77 ± 0.75 mg glucose/g	1409:1438	only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%)	1409:1470	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	2	47	theme	microwave-assisted	375:392	arg1	pretreatment					406:417	microwave-assisted hydrotropic pretreatment	375:417	microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS)	375:454	The study was aimed at evaluating the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS) for the delignification of pine and beech chips and wheat straw.					
35296788	1	48	theme	conversion	246:255	arg1	processes					213:221	processes	213:221	processes of its microbiological conversion e.g.	213:260	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	5	49	from	%	1109:1109	arg1	straw					1120:1124	wheat straw	1114:1124	wheat straw	1114:1124	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	4	50	from	changes	750:756	arg1	composition					765:775	the composition	761:775	the composition of the biomass	761:790	The observed effects included changes in the composition of the biomass and an increased susceptibility of cellulose to the subsequent enzymatic hydrolysis.					
35296788	8	51	theme	main	1916:1919	arg1	component					1929:1937	the main biomass component	1912:1937	the main biomass component	1912:1937	Due to a significant reduction of lignin and hemicellulose concentration in biomass, cellulose-which is susceptible to enzymatic hydrolysis and a source of carbon in biosynthesis processes-becomes the main biomass component.					
35296788	5	52	theme	%	951:951	arg1	NaCS					957:960	40% w/v NaCS	949:960	40% w/v NaCS	949:960	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	7	53	from	delignification	1537:1551	arg1	conditions					1576:1585	the optimum process conditions	1556:1585	the optimum process conditions	1556:1585	Microwave-assisted hydrotropic delignification in the optimum process conditions additionally allows a complete removal of hemicellulose from biomass, which improves the effectiveness of enzymatic hydrolysis.					
35296788	6	54	theme	yield	1247:1251	arg1	%					1265:1265	hydrolysis yield 46.76 ± 1.14%	1236:1265	hydrolysis yield 46.76 ± 1.14%	1236:1265	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	6	54	theme	yield	1247:1251	arg1	glucose/g					1225:1233	glucose: 463.27 ± 11.25 mg glucose/g	1198:1233	glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%)	1198:1266	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	3	55	theme	microwave-assisted	585:602	arg1	delignification					616:630	microwave-assisted hydrotropic delignification	585:630	microwave-assisted hydrotropic delignification	585:630	Research results presenting the impact of process parameters of microwave-assisted hydrotropic delignification confirm a high effectiveness of this method of pretreatment of lignocellulosic biomass.					
35296788	8	56	theme	significant	1724:1734	arg1	reduction					1736:1744	a significant reduction	1722:1744	a significant reduction of lignin and hemicellulose concentration in biomass	1722:1797	Due to a significant reduction of lignin and hemicellulose concentration in biomass, cellulose-which is susceptible to enzymatic hydrolysis and a source of carbon in biosynthesis processes-becomes the main biomass component.					
35296788	1	57	theme	key	190:192	arg1	use					110:112	The use	106:112	The use of a method of an effective delignification of lignocellulosic biomass	106:183	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	1	57	theme	key	190:192	arg1	stage					194:198	a key stage	188:198	a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol	188:317	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	6	58	theme	enzymatic	1133:1141	arg1	hydrolysis					1143:1152	enzymatic hydrolysis	1133:1152	enzymatic hydrolysis	1133:1152	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	3	59	theme	parameters	571:580	arg1	impact					553:558	the impact	549:558	the impact of process parameters of microwave-assisted hydrotropic delignification	549:630	Research results presenting the impact of process parameters of microwave-assisted hydrotropic delignification confirm a high effectiveness of this method of pretreatment of lignocellulosic biomass.					
35296788	6	60	theme	pine	1494:1497	arg1	chips					1499:1503	the pine chips	1490:1503	the pine chips	1490:1503	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	5	61	theme	microwave	888:896	arg1	heating					898:904	microwave heating	888:904	microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min	888:983	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	5	62	theme	w/v	953:955	arg1	NaCS					957:960	40% w/v NaCS	949:960	40% w/v NaCS	949:960	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	6	63	theme	327.70 ± 22.15 mg	1309:1325	arg1	%					1367:1367	hydrolysis yield 35.13 ± 2.37%	1338:1367	hydrolysis yield 35.13 ± 2.37%	1338:1367	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	6	63	theme	327.70 ± 22.15 mg	1309:1325	arg1	glucose/g					1327:1335	327.70 ± 22.15 mg glucose/g	1309:1335	327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%)	1309:1368	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	5	64	from	%	1054:1054	arg1	chips					1064:1068	pine chips	1059:1068	pine chips	1059:1068	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	6	65	theme	glucose/g	1225:1233	arg1	concentration					1181:1193	the highest concentration	1169:1193	the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%)	1169:1266	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	3	66	theme	delignification	616:630	arg1	parameters					571:580	process parameters	563:580	process parameters of microwave-assisted hydrotropic delignification	563:630	Research results presenting the impact of process parameters of microwave-assisted hydrotropic delignification confirm a high effectiveness of this method of pretreatment of lignocellulosic biomass.					
35296788	8	67	theme	concentration	1774:1786	arg1	reduction					1736:1744	a significant reduction	1722:1744	a significant reduction of lignin and hemicellulose concentration in biomass	1722:1797	Due to a significant reduction of lignin and hemicellulose concentration in biomass, cellulose-which is susceptible to enzymatic hydrolysis and a source of carbon in biosynthesis processes-becomes the main biomass component.					
35296788	5	68	theme	NaCS	957:960	arg1	hydrotrope					935:944	hydrotrope	935:944	hydrotrope of 40% w/v NaCS and 117 PSI for 60 min	935:983	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	7	69	theme	complete	1609:1616	arg1	removal					1618:1624	a complete removal	1607:1624	a complete removal	1607:1624	Microwave-assisted hydrotropic delignification in the optimum process conditions additionally allows a complete removal of hemicellulose from biomass, which improves the effectiveness of enzymatic hydrolysis.					
35296788	6	70	theme	wheat	1290:1294	arg1	straw					1296:1300	the wheat straw	1286:1300	the wheat straw	1286:1300	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	0	71	theme	hydrotropic	80:90	arg1	pretreatment					92:103	microwave-assisted hydrotropic pretreatment	61:103	microwave-assisted hydrotropic pretreatment	61:103	Delignification efficiency of various types of biomass using microwave-assisted hydrotropic pretreatment.					
35296788	1	72	theme	delignification	142:156	arg1	method					119:124	a method	117:124	a method of an effective delignification of lignocellulosic biomass	117:183	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	2	73	theme	wheat	508:512	arg1	straw					514:518	wheat straw	508:518	wheat straw	508:518	The study was aimed at evaluating the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS) for the delignification of pine and beech chips and wheat straw.					
35296788	6	74	theme	highest	1173:1179	arg1	concentration					1181:1193	the highest concentration	1169:1193	the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%)	1169:1266	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	1	75	theme	lignocellulosic	161:175	arg1	biomass					177:183	lignocellulosic biomass	161:183	lignocellulosic biomass	161:183	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	7	76	theme	optimum	1560:1566	arg1	conditions					1576:1585	the optimum process conditions	1556:1585	the optimum process conditions	1556:1585	Microwave-assisted hydrotropic delignification in the optimum process conditions additionally allows a complete removal of hemicellulose from biomass, which improves the effectiveness of enzymatic hydrolysis.					
35296788	2	77	theme	chips	498:502	arg1	delignification					464:478	the delignification	460:478	the delignification of pine and beech chips	460:502	The study was aimed at evaluating the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS) for the delignification of pine and beech chips and wheat straw.					
35296788	2	77	theme	chips	498:502	arg1	straw					514:518	wheat straw	508:518	wheat straw	508:518	The study was aimed at evaluating the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS) for the delignification of pine and beech chips and wheat straw.					
35296788	8	78	from	source	1861:1866	arg1	biosynthesis					1881:1892	biosynthesis	1881:1892	biosynthesis	1881:1892	Due to a significant reduction of lignin and hemicellulose concentration in biomass, cellulose-which is susceptible to enzymatic hydrolysis and a source of carbon in biosynthesis processes-becomes the main biomass component.					
35296788	8	79	theme	enzymatic	1834:1842	arg1	hydrolysis					1844:1853	enzymatic hydrolysis	1834:1853	enzymatic hydrolysis	1834:1853	Due to a significant reduction of lignin and hemicellulose concentration in biomass, cellulose-which is susceptible to enzymatic hydrolysis and a source of carbon in biosynthesis processes-becomes the main biomass component.					
35296788	5	80	from	%	1079:1079	arg1	chips					1090:1094	beech chips	1084:1094	beech chips	1084:1094	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	4	81	from	susceptibility	809:822	arg1	composition					765:775	the composition	761:775	the composition of the biomass	761:790	The observed effects included changes in the composition of the biomass and an increased susceptibility of cellulose to the subsequent enzymatic hydrolysis.					
35296788	4	82	theme	cellulose	827:835	arg1	changes					750:756	changes	750:756	changes in the composition of the biomass	750:790	The observed effects included changes in the composition of the biomass and an increased susceptibility of cellulose to the subsequent enzymatic hydrolysis.					
35296788	4	82	theme	cellulose	827:835	arg1	susceptibility					809:822	an increased susceptibility	796:822	an increased susceptibility of cellulose to the subsequent enzymatic hydrolysis	796:874	The observed effects included changes in the composition of the biomass and an increased susceptibility of cellulose to the subsequent enzymatic hydrolysis.					
35296788	6	83	theme	yield	1349:1353	arg1	%					1367:1367	hydrolysis yield 35.13 ± 2.37%	1338:1367	hydrolysis yield 35.13 ± 2.37%	1338:1367	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	6	83	theme	yield	1349:1353	arg1	glucose/g					1327:1335	327.70 ± 22.15 mg glucose/g	1309:1335	327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%)	1309:1368	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	7	84	theme	hydrolysis	1703:1712	arg1	effectiveness					1676:1688	the effectiveness	1672:1688	the effectiveness of enzymatic hydrolysis	1672:1712	Microwave-assisted hydrotropic delignification in the optimum process conditions additionally allows a complete removal of hemicellulose from biomass, which improves the effectiveness of enzymatic hydrolysis.					
35296788	4	85	theme	biomass	784:790	arg1	composition					765:775	the composition	761:775	the composition of the biomass	761:790	The observed effects included changes in the composition of the biomass and an increased susceptibility of cellulose to the subsequent enzymatic hydrolysis.					
35296788	2	86	theme	hydrotropic	394:404	arg1	pretreatment					406:417	microwave-assisted hydrotropic pretreatment	375:417	microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS)	375:454	The study was aimed at evaluating the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS) for the delignification of pine and beech chips and wheat straw.					
35296788	0	87	theme	various	30:36	arg1	types					38:42	various types	30:42	various types of biomass	30:53	Delignification efficiency of various types of biomass using microwave-assisted hydrotropic pretreatment.					
35296788	1	88	theme	production	286:295	arg1	purposes					270:277	the purposes	266:277	the purposes of the production of cellulosic ethanol	266:317	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	8	89	theme	carbon	1871:1876	arg1	source					1861:1866	a source	1859:1866	a source of carbon in biosynthesis	1859:1892	Due to a significant reduction of lignin and hemicellulose concentration in biomass, cellulose-which is susceptible to enzymatic hydrolysis and a source of carbon in biosynthesis processes-becomes the main biomass component.					
35296788	1	90	theme	cellulosic	300:309	arg1	ethanol					311:317	cellulosic ethanol	300:317	cellulosic ethanol	300:317	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	2	91	theme	cumene	432:437	arg1	sulfonate					439:447	sodium cumene sulfonate	425:447	sodium cumene sulfonate (NaCS)	425:454	The study was aimed at evaluating the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS) for the delignification of pine and beech chips and wheat straw.					
35296788	2	91	theme	cumene	432:437	arg1	NaCS					450:453	NaCS	450:453	NaCS	450:453	The study was aimed at evaluating the effectiveness of microwave-assisted hydrotropic pretreatment using sodium cumene sulfonate (NaCS) for the delignification of pine and beech chips and wheat straw.					
35296788	6	92	theme	beech	1392:1396	arg1	chips					1398:1402	the beech chips	1388:1402	the beech chips	1388:1402	After enzymatic hydrolysis was conducted, the highest concentration of glucose: 463.27 ± 11.25 mg glucose/g (hydrolysis yield 46.76 ± 1.14%) was obtained from the wheat straw, while 327.70 ± 22.15 mg glucose/g (hydrolysis yield 35.13 ± 2.37%) was acquired from the beech chips, and only 50.77 ± 0.75 mg glucose/g (hydrolysis yield 6.63 ± 0.10%) was obtained from the pine chips.					
35296788	7	93	theme	Microwave-assisted	1506:1523	arg1	delignification					1537:1551	Microwave-assisted hydrotropic delignification	1506:1551	Microwave-assisted hydrotropic delignification in the optimum process conditions	1506:1585	Microwave-assisted hydrotropic delignification in the optimum process conditions additionally allows a complete removal of hemicellulose from biomass, which improves the effectiveness of enzymatic hydrolysis.					
35296788	5	94	theme	concentration	1021:1033	arg1	reduction					995:1003	a reduction	993:1003	a reduction of the absolute concentration of lignins	993:1044	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	3	95	theme	biomass	711:717	arg1	pretreatment					679:690	pretreatment	679:690	pretreatment of lignocellulosic biomass	679:717	Research results presenting the impact of process parameters of microwave-assisted hydrotropic delignification confirm a high effectiveness of this method of pretreatment of lignocellulosic biomass.					
35296788	1	96	theme	microbiological	230:244	arg1	conversion					246:255	its microbiological conversion	226:255	its microbiological conversion	226:255	The use of a method of an effective delignification of lignocellulosic biomass is a key stage of designing processes of its microbiological conversion e.g. for the purposes of the production of cellulosic ethanol.					
35296788	5	97	theme	lignins	1038:1044	arg1	concentration					1021:1033	the absolute concentration	1008:1033	the absolute concentration of lignins	1008:1044	The use of microwave heating combined with an addition of hydrotrope of 40% w/v NaCS and 117 PSI for 60 min enabled a reduction of the absolute concentration of lignins by 36.58% in pine chips, by 57.68% in beech chips, and by 74.08% in wheat straw.					
35296788	4	98	theme	enzymatic	855:863	arg1	hydrolysis					865:874	the subsequent enzymatic hydrolysis	840:874	the subsequent enzymatic hydrolysis	840:874	The observed effects included changes in the composition of the biomass and an increased susceptibility of cellulose to the subsequent enzymatic hydrolysis.					
35614186	0	0	theme	pathogenic	66:75	arg1	biofilm					77:83	in vitro pathogenic biofilm	57:83	in vitro pathogenic biofilm	57:83	The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu.					
35614186	2	1	theme	frequencies	610:620	arg1	field					591:595	the rotating magnetic field	569:595	the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	569:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	2	1	theme	frequencies	610:620	arg1	RMF					598:600	RMF	598:600	RMF	598:600	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	6	2	theme	cell	1260:1263	arg1	wall/membranes					1265:1278	cell wall/membranes	1260:1278	cell wall/membranes	1260:1278	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	1	3	theme	fields	137:142	arg1	application					105:115	The application	101:115	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent	101:263	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	6	4	theme	dihydrochloride	1422:1436	arg1	penetrability					1394:1406	altered penetrability	1386:1406	altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles	1386:1467	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	2	5	from	field	591:595	arg1	model					652:656	the in vitro model	639:656	the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	639:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	2	6	theme	Staphylococcus	492:505	arg1	biofilms					541:548	Staphylococcus aureus and Pseudomonas aeruginosa biofilms	492:548	Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	492:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	0	7	from	effects	4:10	arg1	biofilm					77:83	in vitro pathogenic biofilm	57:83	in vitro pathogenic biofilm	57:83	The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu.					
35614186	0	7	from	effects	4:10	arg1	milieu					93:98	its milieu	89:98	its milieu	89:98	The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu.					
35614186	7	8	theme	antiseptic	1652:1661	arg1	activity					1663:1670	antiseptic activity	1652:1670	antiseptic activity	1652:1670	Our results suggest that the combination of RMF and OCT can be particularly promising in eradicating biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity.					
35614186	2	9	theme	in	643:644	arg1	model					652:656	the in vitro model	639:656	the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	639:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	2	10	dep	in	643:644	arg1	vitro					646:650	vitro	646:650	vitro	646:650	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	1	11	theme	self-reliant	232:243	arg1	agent					259:263	a self-reliant antimicrobial agent	230:263	a self-reliant antimicrobial agent	230:263	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	4	12	theme	octenidine	891:900	arg1	permeability					918:929	octenidine dihydrochloride permeability	891:929	octenidine dihydrochloride permeability through biofilm and porous agar obstacles	891:971	Also, octenidine dihydrochloride permeability through biofilm and porous agar obstacles was determined for the RMF-exposed versus unexposed settings.					
35614186	6	13	theme	performed	1179:1187	arg1	analyses					1189:1196	The performed analyses	1175:1196	The performed analyses	1175:1196	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	1	14	theme	antimicrobial	245:257	arg1	agent					259:263	a self-reliant antimicrobial agent	230:263	a self-reliant antimicrobial agent	230:263	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	4	15	theme	biofilm	939:945	arg1	obstacles					963:971	biofilm and porous agar obstacles	939:971	obstacles	963:971	Also, octenidine dihydrochloride permeability through biofilm and porous agar obstacles was determined for the RMF-exposed versus unexposed settings.					
35614186	7	16	theme	located	1581:1587	arg1	biofilms					1572:1579	biofilms	1572:1579	biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity	1572:1670	Our results suggest that the combination of RMF and OCT can be particularly promising in eradicating biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity.					
35614186	7	17	theme	physical	1627:1634	arg1	obstacles					1636:1644	physical obstacles	1627:1644	physical obstacles	1627:1644	Our results suggest that the combination of RMF and OCT can be particularly promising in eradicating biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity.					
35614186	1	18	theme	agent	259:263	arg1	character					217:225	the character	213:225	the character of a self-reliant antimicrobial agent	213:263	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	4	19	theme	agar	958:961	arg1	obstacles					963:971	biofilm and porous agar obstacles	939:971	obstacles	963:971	Also, octenidine dihydrochloride permeability through biofilm and porous agar obstacles was determined for the RMF-exposed versus unexposed settings.					
35614186	5	20	theme	biofilm	1084:1090	arg1	destruction					1092:1102	biofilm destruction	1084:1102	biofilm destruction	1084:1102	The exposure to RMF or OCT apart did not lead to biofilm destruction, contrary to the setting in which these two agents were used together.					
35614186	7	21	from	areas	1597:1601	arg1	located					1581:1587	located	1581:1587	located	1581:1587	Our results suggest that the combination of RMF and OCT can be particularly promising in eradicating biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity.					
35614186	2	22	theme	increased	412:420	arg1	activity					422:429	increased activity	412:429	increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	412:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	7	23	from	located	1581:1587	arg1	areas					1597:1601	such areas	1592:1601	such areas	1592:1601	Our results suggest that the combination of RMF and OCT can be particularly promising in eradicating biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity.					
35614186	4	24	theme	porous	951:956	arg1	obstacles					963:971	biofilm and porous agar obstacles	939:971	obstacles	963:971	Also, octenidine dihydrochloride permeability through biofilm and porous agar obstacles was determined for the RMF-exposed versus unexposed settings.					
35614186	4	25	theme	RMF-exposed	996:1006	arg1	settings					1025:1032	the RMF-exposed versus unexposed settings	992:1032	the RMF-exposed versus unexposed settings	992:1032	Also, octenidine dihydrochloride permeability through biofilm and porous agar obstacles was determined for the RMF-exposed versus unexposed settings.					
35614186	6	26	theme	altered	1386:1392	arg1	penetrability					1394:1406	altered penetrability	1386:1406	altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles	1386:1467	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	27	theme	biofilm	1320:1326	arg1	porosity					1335:1342	altered biofilm matrix porosity	1312:1342	altered biofilm matrix porosity	1312:1342	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	27	theme	biofilm	1320:1326	arg1	biofilms					1237:1244	biofilms	1237:1244	biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition)	1237:1360	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	2	28	theme	antiseptic	467:476	arg1	OCT					479:481	octenidine dihydrochloride-based antiseptic (OCT)	434:482	octenidine dihydrochloride-based antiseptic (OCT)	434:482	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	2	29	theme	Pseudomonas	518:528	arg1	biofilms					541:548	Staphylococcus aureus and Pseudomonas aeruginosa biofilms	492:548	Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	492:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	6	30	dep	milieu	1378:1383	arg1	penetrability					1394:1406	altered penetrability	1386:1406	altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles	1386:1467	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	2	31	theme	dihydrochloride-based	445:465	arg1	OCT					479:481	octenidine dihydrochloride-based antiseptic (OCT)	434:482	octenidine dihydrochloride-based antiseptic (OCT)	434:482	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	7	32	theme	wound	1606:1610	arg1	pockets					1612:1618	wound pockets	1606:1618	wound pockets	1606:1618	Our results suggest that the combination of RMF and OCT can be particularly promising in eradicating biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity.					
35614186	6	33	theme	RMF	1221:1223	arg1	effect					1211:1216	the effect	1207:1216	the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles)	1207:1468	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	4	34	theme	unexposed	1015:1023	arg1	settings					1025:1032	the RMF-exposed versus unexposed settings	992:1032	the RMF-exposed versus unexposed settings	992:1032	Also, octenidine dihydrochloride permeability through biofilm and porous agar obstacles was determined for the RMF-exposed versus unexposed settings.					
35614186	6	35	theme	cells	1305:1309	arg1	composition					1349:1359	composition	1349:1359	composition	1349:1359	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	35	theme	cells	1305:1309	arg1	porosity					1335:1342	altered biofilm matrix porosity	1312:1342	altered biofilm matrix porosity	1312:1342	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	35	theme	cells	1305:1309	arg1	weakening					1247:1255	weakening	1247:1255	weakening of cell wall/membranes	1247:1278	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	35	theme	cells	1305:1309	arg1	biofilms					1237:1244	biofilms	1237:1244	biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition)	1237:1360	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	35	theme	cells	1305:1309	arg1	morphology					1291:1300	disturbed morphology	1281:1300	disturbed morphology of cells	1281:1309	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	7	36	theme	RMF	1515:1517	arg1	combination					1500:1510	the combination	1496:1510	the combination of RMF and OCT	1496:1525	Our results suggest that the combination of RMF and OCT can be particularly promising in eradicating biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity.					
35614186	7	36	theme	RMF	1515:1517	arg1	promising					1547:1555	promising	1547:1555	promising	1547:1555	Our results suggest that the combination of RMF and OCT can be particularly promising in eradicating biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity.					
35614186	0	37	theme	rotating	15:22	arg1	field					33:37	rotating magnetic field	15:37	rotating magnetic field	15:37	The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu.					
35614186	6	38	theme	wall/membranes	1265:1278	arg1	composition					1349:1359	composition	1349:1359	composition	1349:1359	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	38	theme	wall/membranes	1265:1278	arg1	porosity					1335:1342	altered biofilm matrix porosity	1312:1342	altered biofilm matrix porosity	1312:1342	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	38	theme	wall/membranes	1265:1278	arg1	weakening					1247:1255	weakening	1247:1255	weakening of cell wall/membranes	1247:1278	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	38	theme	wall/membranes	1265:1278	arg1	biofilms					1237:1244	biofilms	1237:1244	biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition)	1237:1360	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	38	theme	wall/membranes	1265:1278	arg1	morphology					1291:1300	disturbed morphology	1281:1300	disturbed morphology of cells	1281:1309	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	2	39	theme	rotating	573:580	arg1	field					591:595	the rotating magnetic field	569:595	the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	569:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	2	39	theme	rotating	573:580	arg1	RMF					598:600	RMF	598:600	RMF	598:600	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	0	40	dep	in	57:58	arg1	vitro					60:64	vitro	60:64	vitro	60:64	The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu.					
35614186	2	41	theme	solution	756:763	arg1	source					731:736	the source	727:736	the source of the antiseptic solution	727:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	1	42	theme	different	173:181	arg1	molecules					197:205	different antimicrobial molecules	173:205	different antimicrobial molecules	173:205	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	2	43	from	frequencies	610:620	arg1	model					652:656	the in vitro model	639:656	the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	639:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	2	44	theme	study	373:377	arg1	purpose					357:363	The purpose	353:363	The purpose of this study	353:377	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	6	45	dep	biofilms	1237:1244	arg1	composition					1349:1359	composition	1349:1359	composition	1349:1359	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	45	dep	biofilms	1237:1244	arg1	porosity					1335:1342	altered biofilm matrix porosity	1312:1342	altered biofilm matrix porosity	1312:1342	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	45	dep	biofilms	1237:1244	arg1	weakening					1247:1255	weakening	1247:1255	weakening of cell wall/membranes	1247:1278	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	45	dep	biofilms	1237:1244	arg1	biofilms					1237:1244	biofilms	1237:1244	biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition)	1237:1360	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	45	dep	biofilms	1237:1244	arg1	morphology					1291:1300	disturbed morphology	1281:1300	disturbed morphology of cells	1281:1309	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	2	46	theme	antiseptic	745:754	arg1	solution					756:763	the antiseptic solution	741:763	the antiseptic solution	741:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	1	47	theme	antimicrobial	183:195	arg1	molecules					197:205	different antimicrobial molecules	173:205	different antimicrobial molecules	173:205	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	0	48	theme	field	33:37	arg1	effects					4:10	The effects	0:10	The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu	0:98	The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu.					
35614186	1	49	theme	promising	281:289	arg1	approach					291:298	a promising approach	279:298	a promising approach to eradicating bacterial biofilm-related infections	279:350	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	6	50	theme	octenidine	1411:1420	arg1	dihydrochloride					1422:1436	octenidine dihydrochloride	1411:1436	octenidine dihydrochloride through biofilm/agar obstacles	1411:1467	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	51	from	effect	1211:1216	arg1	composition					1349:1359	composition	1349:1359	composition	1349:1359	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	51	from	effect	1211:1216	arg1	milieu					1378:1383	its milieu	1374:1383	its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles)	1374:1468	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	51	from	effect	1211:1216	arg1	biofilms					1237:1244	biofilms	1237:1244	biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition)	1237:1360	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	51	from	effect	1211:1216	arg1	porosity					1335:1342	altered biofilm matrix porosity	1312:1342	altered biofilm matrix porosity	1312:1342	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	51	from	effect	1211:1216	arg1	weakening					1247:1255	weakening	1247:1255	weakening of cell wall/membranes	1247:1278	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	51	from	effect	1211:1216	arg1	morphology					1291:1300	disturbed morphology	1281:1300	disturbed morphology of cells	1281:1309	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	1	52	theme	molecules	197:205	arg1	efficacy					161:168	the efficacy	157:168	the efficacy of different antimicrobial molecules	157:205	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	0	53	theme	magnetic	24:31	arg1	field					33:37	rotating magnetic field	15:37	rotating magnetic field	15:37	The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu.					
35614186	3	54	dep	cells	786:790	arg1	cells					786:790	The biofilm-forming cells	766:790	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition	766:868	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition were analyzed.					
35614186	3	54	dep	cells	786:790	arg1	composition					858:868	composition	858:868	composition	858:868	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition were analyzed.					
35614186	3	54	dep	cells	786:790	arg1	morphology					807:816	morphology	807:816	morphology	807:816	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition were analyzed.					
35614186	3	54	dep	cells	786:790	arg1	structure					844:852	structure	844:852	structure	844:852	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition were analyzed.					
35614186	3	54	dep	cells	786:790	arg1	viability					793:801	viability	793:801	viability	793:801	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition were analyzed.					
35614186	2	55	theme	octenidine	434:443	arg1	OCT					479:481	octenidine dihydrochloride-based antiseptic (OCT)	434:482	octenidine dihydrochloride-based antiseptic (OCT)	434:482	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	6	56	theme	altered	1312:1318	arg1	porosity					1335:1342	altered biofilm matrix porosity	1312:1342	altered biofilm matrix porosity	1312:1342	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	56	theme	altered	1312:1318	arg1	biofilms					1237:1244	biofilms	1237:1244	biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition)	1237:1360	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	0	57	theme	antiseptic	43:52	arg1	effects					4:10	The effects	0:10	The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu	0:98	The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu.					
35614186	7	58	theme	such	1592:1595	arg1	areas					1597:1601	such areas	1592:1601	such areas	1592:1601	Our results suggest that the combination of RMF and OCT can be particularly promising in eradicating biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity.					
35614186	6	59	theme	disturbed	1281:1289	arg1	biofilms					1237:1244	biofilms	1237:1244	biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition)	1237:1360	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	59	theme	disturbed	1281:1289	arg1	morphology					1291:1300	disturbed morphology	1281:1300	disturbed morphology of cells	1281:1309	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	2	60	dep	Pseudomonas	518:528	arg1	aeruginosa					530:539	aeruginosa	530:539	aeruginosa	530:539	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	1	61	theme	eradicating	303:313	arg1	infections					341:350	eradicating bacterial biofilm-related infections	303:350	eradicating bacterial biofilm-related infections	303:350	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	2	62	dep	Staphylococcus	492:505	arg1	aureus					507:512	aureus	507:512	aureus	507:512	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	2	63	from	biofilms	541:548	arg1	presence					557:564	the presence	553:564	the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	553:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	2	64	theme	agar	680:683	arg1	discs					685:689	stacked agar discs	672:689	stacked agar discs	672:689	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	3	65	theme	biofilm	829:835	arg1	matrix					837:842	biofilm matrix	829:842	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition	766:868	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition were analyzed.					
35614186	1	66	theme	bacterial	315:323	arg1	infections					341:350	eradicating bacterial biofilm-related infections	303:350	eradicating bacterial biofilm-related infections	303:350	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	2	67	theme	field	591:595	arg1	presence					557:564	the presence	553:564	the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	553:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	5	68	used	used	1160:1163	arg2	agents					1148:1153	these two agents	1138:1153	these two agents	1138:1153	The exposure to RMF or OCT apart did not lead to biofilm destruction, contrary to the setting in which these two agents were used together.					
35614186	2	69	theme	stacked	672:678	arg1	discs					685:689	stacked agar discs	672:689	stacked agar discs	672:689	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	2	70	theme	magnetic	582:589	arg1	field					591:595	the rotating magnetic field	569:595	the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	569:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	2	70	theme	magnetic	582:589	arg1	RMF					598:600	RMF	598:600	RMF	598:600	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	2	71	from	model	652:656	arg1	field					591:595	the rotating magnetic field	569:595	the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	569:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	2	71	from	model	652:656	arg1	RMF					598:600	RMF	598:600	RMF	598:600	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	6	72	theme	matrix	1328:1333	arg1	porosity					1335:1342	altered biofilm matrix porosity	1312:1342	altered biofilm matrix porosity	1312:1342	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	6	72	theme	matrix	1328:1333	arg1	biofilms					1237:1244	biofilms	1237:1244	biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition)	1237:1360	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	0	73	theme	in	57:58	arg1	biofilm					77:83	in vitro pathogenic biofilm	57:83	in vitro pathogenic biofilm	57:83	The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu.					
35614186	4	74	theme	dihydrochloride	902:916	arg1	permeability					918:929	octenidine dihydrochloride permeability	891:929	octenidine dihydrochloride permeability through biofilm and porous agar obstacles	891:971	Also, octenidine dihydrochloride permeability through biofilm and porous agar obstacles was determined for the RMF-exposed versus unexposed settings.					
35614186	7	75	theme	OCT	1523:1525	arg1	combination					1500:1510	the combination	1496:1510	the combination of RMF and OCT	1496:1525	Our results suggest that the combination of RMF and OCT can be particularly promising in eradicating biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity.					
35614186	7	75	theme	OCT	1523:1525	arg1	promising					1547:1555	promising	1547:1555	promising	1547:1555	Our results suggest that the combination of RMF and OCT can be particularly promising in eradicating biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity.					
35614186	2	76	theme	activity	422:429	arg1	phenomenon					398:407	the phenomenon	394:407	the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	394:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	1	77	theme	various	120:126	arg1	fields					137:142	various magnetic fields	120:142	various magnetic fields	120:142	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	6	78	theme	biofilm/agar	1446:1457	arg1	obstacles					1459:1467	biofilm/agar obstacles	1446:1467	biofilm/agar obstacles	1446:1467	The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles).					
35614186	2	79	theme	OCT	479:481	arg1	activity					422:429	increased activity	412:429	increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution	412:763	The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution.					
35614186	1	80	theme	biofilm-related	325:339	arg1	infections					341:350	eradicating bacterial biofilm-related infections	303:350	eradicating bacterial biofilm-related infections	303:350	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	1	81	theme	magnetic	128:135	arg1	fields					137:142	various magnetic fields	120:142	various magnetic fields	120:142	The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections.					
35614186	3	82	theme	biofilm-forming	770:784	arg1	cells					786:790	The biofilm-forming cells	766:790	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition	766:868	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition were analyzed.					
35614186	3	82	theme	biofilm-forming	770:784	arg1	composition					858:868	composition	858:868	composition	858:868	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition were analyzed.					
35614186	3	82	theme	biofilm-forming	770:784	arg1	morphology					807:816	morphology	807:816	morphology	807:816	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition were analyzed.					
35614186	3	82	theme	biofilm-forming	770:784	arg1	structure					844:852	structure	844:852	structure	844:852	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition were analyzed.					
35614186	3	82	theme	biofilm-forming	770:784	arg1	viability					793:801	viability	793:801	viability	793:801	The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition were analyzed.					
35795888	2	0	theme	simulated	543:551	arg1	SBF					565:567	SBF	565:567	SBF	565:567	Lys-Ce-MSNs raise the mechanical stiffness of the hydrogel composite scaffold and induce surface apatite mineralization, when the scaffold is immersed in simulated body fluid (SBF).					
35795888	2	0	theme	simulated	543:551	arg1	fluid					558:562	simulated body fluid	543:562	simulated body fluid (SBF)	543:568	Lys-Ce-MSNs raise the mechanical stiffness of the hydrogel composite scaffold and induce surface apatite mineralization, when the scaffold is immersed in simulated body fluid (SBF).					
35795888	5	1	dep	in	821:822	arg1	vitro					824:828	vitro	824:828	vitro	824:828	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	1	2	theme	novel	172:176	arg1	biomaterial					178:188	A novel biomaterial	170:188	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs)	170:342	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	8	3	theme	hydrogel	1525:1532	arg1	BTE					1559:1561	BTE	1559:1561	BTE	1559:1561	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	8	3	theme	hydrogel	1525:1532	arg1	engineering					1546:1556	multifunctional hydrogel bone tissue engineering	1509:1556	multifunctional hydrogel bone tissue engineering (BTE) scaffolds	1509:1572	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	1	4	theme	cerium	289:294	arg1	Lys-Ce-MSNs					331:341	Lys-Ce-MSNs	331:341	Lys-Ce-MSNs	331:341	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	1	4	theme	cerium	289:294	arg1	nanoparticles					316:328	lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles	262:328	lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs)	262:342	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	3	5	theme	antibacterial	681:693	arg1	properties					724:733	antibacterial (and potentially anticancer) properties	681:733	antibacterial (and potentially anticancer) properties	681:733	Moreover, the scaffolds can co-deliver bone healing (Ca and Si) and antioxidant ions (Ce), and Lys to achieve antibacterial (and potentially anticancer) properties.					
35795888	1	6	theme	doped	296:300	arg1	Lys-Ce-MSNs					331:341	Lys-Ce-MSNs	331:341	Lys-Ce-MSNs	331:341	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	1	6	theme	doped	296:300	arg1	nanoparticles					316:328	lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles	262:328	lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs)	262:342	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	0	7	theme	Lysozyme	101:108	arg1	Cerium					117:122	Lysozyme Loaded Cerium	101:122	Lysozyme Loaded Cerium Doped Mesoporous Silica-Calcia Nanoparticles	101:167	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine Reinforced by Lysozyme Loaded Cerium Doped Mesoporous Silica-Calcia Nanoparticles.					
35795888	5	8	from	structure	1092:1100	arg1	biocompatibility					989:1004	the biocompatibility	985:1004	the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure	985:1100	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	1	9	theme	silica-calcia	302:314	arg1	Lys-Ce-MSNs					331:341	Lys-Ce-MSNs	331:341	Lys-Ce-MSNs	331:341	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	1	9	theme	silica-calcia	302:314	arg1	nanoparticles					316:328	lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles	262:328	lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs)	262:342	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	4	10	theme	nanocomposite	740:752	arg1	scaffolds					763:771	The nanocomposite hydrogel scaffolds	736:771	The nanocomposite hydrogel scaffolds	736:771	The nanocomposite hydrogel scaffolds can hold and deliver Lys steadily.					
35795888	5	11	theme	scaffold	1083:1090	arg1	structure					1092:1100	the scaffold structure	1079:1100	the scaffold structure	1079:1100	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	6	12	theme	cells	1167:1171	arg1	level					1140:1144	the proliferation level	1122:1144	the proliferation level of MG63 osteosarcoma cells	1122:1171	On the other hand, the proliferation level of MG63 osteosarcoma cells decreased, likely due to the effect of Lys.					
35795888	5	13	contain	containing	866:875	arg2	Lys					877:879	Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation,	877:973	Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation,	877:973	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	13	contain	containing	866:875	arg1	nanocomposite					852:864	the hydrogel nanocomposite	839:864	the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation,	839:973	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	14	theme	cell	913:916	arg1	proliferation					929:941	assured improved pre-osteoblast cell (MC3T3-E1) proliferation	881:941	assured improved pre-osteoblast cell (MC3T3-E1) proliferation	881:941	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	15	from	ADA-GEL	1009:1015	arg1	structure					1092:1100	the scaffold structure	1079:1100	the scaffold structure	1079:1100	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	8	16	theme	multifunctional	1509:1523	arg1	BTE					1559:1561	BTE	1559:1561	BTE	1559:1561	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	8	16	theme	multifunctional	1509:1523	arg1	engineering					1546:1556	multifunctional hydrogel bone tissue engineering	1509:1556	multifunctional hydrogel bone tissue engineering (BTE) scaffolds	1509:1572	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	6	17	theme	osteosarcoma	1154:1165	arg1	cells					1167:1171	MG63 osteosarcoma cells	1149:1171	MG63 osteosarcoma cells	1149:1171	On the other hand, the proliferation level of MG63 osteosarcoma cells decreased, likely due to the effect of Lys.					
35795888	2	18	theme	composite	448:456	arg1	scaffold					458:465	the hydrogel composite scaffold	435:465	the hydrogel composite scaffold	435:465	Lys-Ce-MSNs raise the mechanical stiffness of the hydrogel composite scaffold and induce surface apatite mineralization, when the scaffold is immersed in simulated body fluid (SBF).					
35795888	0	19	theme	Loaded	110:115	arg1	Cerium					117:122	Lysozyme Loaded Cerium	101:122	Lysozyme Loaded Cerium Doped Mesoporous Silica-Calcia Nanoparticles	101:167	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine Reinforced by Lysozyme Loaded Cerium Doped Mesoporous Silica-Calcia Nanoparticles.					
35795888	3	20	theme	anticancer	712:721	arg1	properties					724:733	antibacterial (and potentially anticancer) properties	681:733	antibacterial (and potentially anticancer) properties	681:733	Moreover, the scaffolds can co-deliver bone healing (Ca and Si) and antioxidant ions (Ce), and Lys to achieve antibacterial (and potentially anticancer) properties.					
35795888	6	21	theme	MG63	1149:1152	arg1	cells					1167:1171	MG63 osteosarcoma cells	1149:1171	MG63 osteosarcoma cells	1149:1171	On the other hand, the proliferation level of MG63 osteosarcoma cells decreased, likely due to the effect of Lys.					
35795888	5	22	from	biocompatibility	989:1004	arg1	structure					1092:1100	the scaffold structure	1079:1100	the scaffold structure	1079:1100	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	1	23	theme	alginate	201:208	arg1	hydrogel					240:247	alginate dialdehyde-gelatine (ADA-GEL) hydrogel	201:247	alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs)	201:342	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	0	24	theme	3D	2:3	arg1	Engineering					25:35	A 3D Printed Bone Tissue Engineering	0:35	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine	0:85	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine Reinforced by Lysozyme Loaded Cerium Doped Mesoporous Silica-Calcia Nanoparticles.					
35795888	8	25	theme	bone	1534:1537	arg1	BTE					1559:1561	BTE	1559:1561	BTE	1559:1561	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	8	25	theme	bone	1534:1537	arg1	engineering					1546:1556	multifunctional hydrogel bone tissue engineering	1509:1556	multifunctional hydrogel bone tissue engineering (BTE) scaffolds	1509:1572	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	3	26	theme	bone	610:613	arg1	Si					631:632	Ca and Si	624:632	Si	631:632	Moreover, the scaffolds can co-deliver bone healing (Ca and Si) and antioxidant ions (Ce), and Lys to achieve antibacterial (and potentially anticancer) properties.					
35795888	3	26	theme	bone	610:613	arg1	Ca					624:625	Ca and Si	624:632	Ca	624:625	Moreover, the scaffolds can co-deliver bone healing (Ca and Si) and antioxidant ions (Ce), and Lys to achieve antibacterial (and potentially anticancer) properties.					
35795888	3	26	theme	bone	610:613	arg1	healing					615:621	bone healing	610:621	bone healing (Ca and Si)	610:633	Moreover, the scaffolds can co-deliver bone healing (Ca and Si) and antioxidant ions (Ce), and Lys to achieve antibacterial (and potentially anticancer) properties.					
35795888	1	27	theme	dialdehyde-gelatine	210:228	arg1	hydrogel					240:247	alginate dialdehyde-gelatine (ADA-GEL) hydrogel	201:247	alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs)	201:342	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	5	28	theme	effect	1062:1067	arg1	biocompatibility					989:1004	the biocompatibility	985:1004	the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure	985:1100	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	7	29	theme	gram-positive	1379:1391	arg1	bacteria					1411:1418	gram-positive and gram-negative bacteria	1379:1418	gram-positive and gram-negative bacteria	1379:1418	Last but not least, cooperatively, alongside gentamicin (GEN), Lys brought about a proper antibacterial efficiency to the hydrogel nanocomposite scaffold against gram-positive and gram-negative bacteria.					
35795888	5	30	theme	assured	881:887	arg1	proliferation					929:941	assured improved pre-osteoblast cell (MC3T3-E1) proliferation	881:941	assured improved pre-osteoblast cell (MC3T3-E1) proliferation	881:941	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	0	31	theme	Bone	13:16	arg1	Engineering					25:35	A 3D Printed Bone Tissue Engineering	0:35	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine	0:85	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine Reinforced by Lysozyme Loaded Cerium Doped Mesoporous Silica-Calcia Nanoparticles.					
35795888	8	32	theme	engineering	1546:1556	arg1	scaffolds					1564:1572	multifunctional hydrogel bone tissue engineering (BTE) scaffolds	1509:1572	multifunctional hydrogel bone tissue engineering (BTE) scaffolds	1509:1572	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	0	33	theme	Mesoporous	130:139	arg1	Nanoparticles					155:167	Mesoporous Silica-Calcia Nanoparticles	130:167	Mesoporous Silica-Calcia Nanoparticles	130:167	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine Reinforced by Lysozyme Loaded Cerium Doped Mesoporous Silica-Calcia Nanoparticles.					
35795888	6	34	theme	proliferation	1126:1138	arg1	level					1140:1144	the proliferation level	1122:1144	the proliferation level of MG63 osteosarcoma cells	1122:1171	On the other hand, the proliferation level of MG63 osteosarcoma cells decreased, likely due to the effect of Lys.					
35795888	5	35	dep	Lys	877:879	arg1	adhesion					944:951	adhesion	944:951	adhesion	944:951	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	35	dep	Lys	877:879	arg1	differentiation					958:972	differentiation	958:972	differentiation	958:972	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	35	dep	Lys	877:879	arg1	proliferation					929:941	assured improved pre-osteoblast cell (MC3T3-E1) proliferation	881:941	assured improved pre-osteoblast cell (MC3T3-E1) proliferation	881:941	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	2	36	theme	surface	478:484	arg1	mineralization					494:507	surface apatite mineralization	478:507	surface apatite mineralization	478:507	Lys-Ce-MSNs raise the mechanical stiffness of the hydrogel composite scaffold and induce surface apatite mineralization, when the scaffold is immersed in simulated body fluid (SBF).					
35795888	0	37	theme	Printed	5:11	arg1	Engineering					25:35	A 3D Printed Bone Tissue Engineering	0:35	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine	0:85	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine Reinforced by Lysozyme Loaded Cerium Doped Mesoporous Silica-Calcia Nanoparticles.					
35795888	8	38	theme	great	1476:1480	arg1	promise					1482:1488	great promise	1476:1488	great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds	1476:1572	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	4	39	theme	hydrogel	754:761	arg1	scaffolds					763:771	The nanocomposite hydrogel scaffolds	736:771	The nanocomposite hydrogel scaffolds	736:771	The nanocomposite hydrogel scaffolds can hold and deliver Lys steadily.					
35795888	5	40	from	bioactivity	1018:1028	arg1	structure					1092:1100	the scaffold structure	1079:1100	the scaffold structure	1079:1100	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	0	41	theme	Engineering	25:35	arg1	Scaffold					37:44	A 3D Printed Bone Tissue Engineering Scaffold	0:44	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine	0:85	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine Reinforced by Lysozyme Loaded Cerium Doped Mesoporous Silica-Calcia Nanoparticles.					
35795888	5	42	theme	Lys	1072:1074	arg1	ADA-GEL					1009:1015	ADA-GEL	1009:1015	ADA-GEL	1009:1015	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	42	theme	Lys	1072:1074	arg1	effect					1062:1067	the stabilizing effect	1046:1067	the stabilizing effect of Lys on the scaffold structure	1046:1100	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	42	theme	Lys	1072:1074	arg1	bioactivity					1018:1028	bioactivity	1018:1028	bioactivity of Ce-MSNs	1018:1039	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	43	theme	bioactivity	1018:1028	arg1	biocompatibility					989:1004	the biocompatibility	985:1004	the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure	985:1100	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	8	44	theme	tumor	1652:1656	arg1	formation					1658:1666	tumor formation	1652:1666	tumor formation	1652:1666	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	6	45	theme	Lys	1212:1214	arg1	effect					1202:1207	the effect	1198:1207	the effect of Lys	1198:1214	On the other hand, the proliferation level of MG63 osteosarcoma cells decreased, likely due to the effect of Lys.					
35795888	0	46	theme	Tissue	18:23	arg1	Engineering					25:35	A 3D Printed Bone Tissue Engineering	0:35	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine	0:85	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine Reinforced by Lysozyme Loaded Cerium Doped Mesoporous Silica-Calcia Nanoparticles.					
35795888	0	47	theme	Silica-Calcia	141:153	arg1	Nanoparticles					155:167	Mesoporous Silica-Calcia Nanoparticles	130:167	Mesoporous Silica-Calcia Nanoparticles	130:167	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine Reinforced by Lysozyme Loaded Cerium Doped Mesoporous Silica-Calcia Nanoparticles.					
35795888	8	48	theme	ADA-GEL/Lys-Ce-MSN	1437:1454	arg1	nanocomposite					1456:1468	ADA-GEL/Lys-Ce-MSN nanocomposite	1437:1468	ADA-GEL/Lys-Ce-MSN nanocomposite	1437:1468	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	5	49	theme	in	821:822	arg1	results					830:836	the in vitro results	817:836	the in vitro results	817:836	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	2	50	theme	scaffold	458:465	arg1	stiffness					422:430	the mechanical stiffness	407:430	the mechanical stiffness of the hydrogel composite scaffold	407:465	Lys-Ce-MSNs raise the mechanical stiffness of the hydrogel composite scaffold and induce surface apatite mineralization, when the scaffold is immersed in simulated body fluid (SBF).					
35795888	8	51	theme	scaffolds	1564:1572	arg1	promise					1482:1488	great promise	1476:1488	great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds	1476:1572	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	5	52	from	effect	1062:1067	arg1	structure					1092:1100	the scaffold structure	1079:1100	the scaffold structure	1079:1100	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	53	theme	Ce-MSNs	1033:1039	arg1	ADA-GEL					1009:1015	ADA-GEL	1009:1015	ADA-GEL	1009:1015	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	53	theme	Ce-MSNs	1033:1039	arg1	effect					1062:1067	the stabilizing effect	1046:1067	the stabilizing effect of Lys on the scaffold structure	1046:1100	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	53	theme	Ce-MSNs	1033:1039	arg1	bioactivity					1018:1028	bioactivity	1018:1028	bioactivity of Ce-MSNs	1018:1039	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	8	54	theme	tissue	1539:1544	arg1	BTE					1559:1561	BTE	1559:1561	BTE	1559:1561	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	8	54	theme	tissue	1539:1544	arg1	engineering					1546:1556	multifunctional hydrogel bone tissue engineering	1509:1556	multifunctional hydrogel bone tissue engineering (BTE) scaffolds	1509:1572	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	8	55	theme	3D	1494:1495	arg1	printing					1497:1504	3D printing	1494:1504	3D printing	1494:1504	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	7	56	theme	proper	1300:1305	arg1	efficiency					1321:1330	a proper antibacterial efficiency	1298:1330	a proper antibacterial efficiency to the hydrogel nanocomposite scaffold against gram-positive and gram-negative bacteria	1298:1418	Last but not least, cooperatively, alongside gentamicin (GEN), Lys brought about a proper antibacterial efficiency to the hydrogel nanocomposite scaffold against gram-positive and gram-negative bacteria.					
35795888	3	57	theme	antioxidant	639:649	arg1	Ce					657:658	Ce	657:658	Ce	657:658	Moreover, the scaffolds can co-deliver bone healing (Ca and Si) and antioxidant ions (Ce), and Lys to achieve antibacterial (and potentially anticancer) properties.					
35795888	3	57	theme	antioxidant	639:649	arg1	ions					651:654	antioxidant ions	639:654	antioxidant ions (Ce)	639:659	Moreover, the scaffolds can co-deliver bone healing (Ca and Si) and antioxidant ions (Ce), and Lys to achieve antibacterial (and potentially anticancer) properties.					
35795888	7	58	theme	hydrogel	1339:1346	arg1	scaffold					1362:1369	the hydrogel nanocomposite scaffold	1335:1369	the hydrogel nanocomposite scaffold	1335:1369	Last but not least, cooperatively, alongside gentamicin (GEN), Lys brought about a proper antibacterial efficiency to the hydrogel nanocomposite scaffold against gram-positive and gram-negative bacteria.					
35795888	6	59	theme	other	1110:1114	arg1	hand					1116:1119	the other hand	1106:1119	the other hand	1106:1119	On the other hand, the proliferation level of MG63 osteosarcoma cells decreased, likely due to the effect of Lys.					
35795888	7	60	theme	nanocomposite	1348:1360	arg1	scaffold					1362:1369	the hydrogel nanocomposite scaffold	1335:1369	the hydrogel nanocomposite scaffold	1335:1369	Last but not least, cooperatively, alongside gentamicin (GEN), Lys brought about a proper antibacterial efficiency to the hydrogel nanocomposite scaffold against gram-positive and gram-negative bacteria.					
35795888	8	61	theme	bone	1590:1593	arg1	regeneration					1595:1606	bone regeneration	1590:1606	bone regeneration	1590:1606	Taken together, ADA-GEL/Lys-Ce-MSN nanocomposite holds great promise for 3D printing of multifunctional hydrogel bone tissue engineering (BTE) scaffolds, able to induce bone regeneration, address infection, and potentially inhibit tumor formation and growth.					
35795888	7	62	theme	gram-negative	1397:1409	arg1	bacteria					1411:1418	gram-positive and gram-negative bacteria	1379:1418	gram-positive and gram-negative bacteria	1379:1418	Last but not least, cooperatively, alongside gentamicin (GEN), Lys brought about a proper antibacterial efficiency to the hydrogel nanocomposite scaffold against gram-positive and gram-negative bacteria.					
35795888	5	63	theme	stabilizing	1050:1060	arg1	effect					1062:1067	the stabilizing effect	1046:1067	the stabilizing effect of Lys on the scaffold structure	1046:1100	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	7	64	theme	antibacterial	1307:1319	arg1	efficiency					1321:1330	a proper antibacterial efficiency	1298:1330	a proper antibacterial efficiency to the hydrogel nanocomposite scaffold against gram-positive and gram-negative bacteria	1298:1418	Last but not least, cooperatively, alongside gentamicin (GEN), Lys brought about a proper antibacterial efficiency to the hydrogel nanocomposite scaffold against gram-positive and gram-negative bacteria.					
35795888	5	65	theme	pre-osteoblast	898:911	arg1	cell					913:916	improved pre-osteoblast cell	889:916	assured improved pre-osteoblast cell (MC3T3-E1) proliferation	881:941	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	65	theme	pre-osteoblast	898:911	arg1	MC3T3-E1					919:926	MC3T3-E1	919:926	MC3T3-E1	919:926	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	1	66	theme	lysozyme	262:269	arg1	Lys-Ce-MSNs					331:341	Lys-Ce-MSNs	331:341	Lys-Ce-MSNs	331:341	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	1	66	theme	lysozyme	262:269	arg1	nanoparticles					316:328	lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles	262:328	lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs)	262:342	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	0	67	theme	Alginate	58:65	arg1	Dialdehyde-Gelatine					67:85	Alginate Dialdehyde-Gelatine	58:85	Alginate Dialdehyde-Gelatine	58:85	A 3D Printed Bone Tissue Engineering Scaffold Composed of Alginate Dialdehyde-Gelatine Reinforced by Lysozyme Loaded Cerium Doped Mesoporous Silica-Calcia Nanoparticles.					
35795888	5	68	theme	hydrogel	843:850	arg1	nanocomposite					852:864	the hydrogel nanocomposite	839:864	the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation,	839:973	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	69	theme	ADA-GEL	1009:1015	arg1	biocompatibility					989:1004	the biocompatibility	985:1004	the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure	985:1100	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	1	70	theme	ADA-GEL	231:237	arg1	hydrogel					240:247	alginate dialdehyde-gelatine (ADA-GEL) hydrogel	201:247	alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs)	201:342	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	5	71	theme	improved	889:896	arg1	cell					913:916	improved pre-osteoblast cell	889:916	assured improved pre-osteoblast cell (MC3T3-E1) proliferation	881:941	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	5	71	theme	improved	889:896	arg1	MC3T3-E1					919:926	MC3T3-E1	919:926	MC3T3-E1	919:926	Based on the in vitro results, the hydrogel nanocomposite containing Lys assured improved pre-osteoblast cell (MC3T3-E1) proliferation, adhesion, and differentiation, thanks to the biocompatibility of ADA-GEL, bioactivity of Ce-MSNs, and the stabilizing effect of Lys on the scaffold structure.					
35795888	2	72	theme	hydrogel	439:446	arg1	scaffold					458:465	the hydrogel composite scaffold	435:465	the hydrogel composite scaffold	435:465	Lys-Ce-MSNs raise the mechanical stiffness of the hydrogel composite scaffold and induce surface apatite mineralization, when the scaffold is immersed in simulated body fluid (SBF).					
35795888	1	73	theme	loaded	271:276	arg1	Lys-Ce-MSNs					331:341	Lys-Ce-MSNs	331:341	Lys-Ce-MSNs	331:341	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	1	73	theme	loaded	271:276	arg1	nanoparticles					316:328	lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles	262:328	lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs)	262:342	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	2	74	theme	body	553:556	arg1	SBF					565:567	SBF	565:567	SBF	565:567	Lys-Ce-MSNs raise the mechanical stiffness of the hydrogel composite scaffold and induce surface apatite mineralization, when the scaffold is immersed in simulated body fluid (SBF).					
35795888	2	74	theme	body	553:556	arg1	fluid					558:562	simulated body fluid	543:562	simulated body fluid (SBF)	543:568	Lys-Ce-MSNs raise the mechanical stiffness of the hydrogel composite scaffold and induce surface apatite mineralization, when the scaffold is immersed in simulated body fluid (SBF).					
35795888	1	75	theme	bioactive	368:376	arg1	scaffolds					378:386	bioactive scaffolds	368:386	bioactive scaffolds	368:386	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	2	76	theme	apatite	486:492	arg1	mineralization					494:507	surface apatite mineralization	478:507	surface apatite mineralization	478:507	Lys-Ce-MSNs raise the mechanical stiffness of the hydrogel composite scaffold and induce surface apatite mineralization, when the scaffold is immersed in simulated body fluid (SBF).					
35795888	2	77	theme	mechanical	411:420	arg1	stiffness					422:430	the mechanical stiffness	407:430	the mechanical stiffness of the hydrogel composite scaffold	407:465	Lys-Ce-MSNs raise the mechanical stiffness of the hydrogel composite scaffold and induce surface apatite mineralization, when the scaffold is immersed in simulated body fluid (SBF).					
35795888	1	78	theme	mesoporous	278:287	arg1	Lys-Ce-MSNs					331:341	Lys-Ce-MSNs	331:341	Lys-Ce-MSNs	331:341	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
35795888	1	78	theme	mesoporous	278:287	arg1	nanoparticles					316:328	lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles	262:328	lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs)	262:342	A novel biomaterial comprising alginate dialdehyde-gelatine (ADA-GEL) hydrogel augmented by lysozyme loaded mesoporous cerium doped silica-calcia nanoparticles (Lys-Ce-MSNs) is 3D printed to create bioactive scaffolds.					
36772992	7	0	from	brands	1163:1168	arg1	richness					1173:1180	richness	1173:1180	richness	1173:1180	16 S rRNA sequencing revealed no differences between brands in richness or overall composition.					
36772992	7	0	from	brands	1163:1168	arg1	composition					1193:1203	overall composition	1185:1203	overall composition	1185:1203	16 S rRNA sequencing revealed no differences between brands in richness or overall composition.					
36772992	3	1	theme	viscoelastic	561:572	arg1	material					574:581	viscoelastic material	561:581	viscoelastic material from the same three manufacturers	561:615	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	1	2	theme	endotoxin	261:269	arg1	quantification					271:284	viable bacteria and endotoxin quantification	241:284	viable bacteria and endotoxin quantification	241:284	PURPOSE To survey commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification.					
36772992	4	3	theme	Statistical	730:740	arg1	analysis					742:749	Statistical analysis	730:749	Statistical analysis	730:749	Statistical analysis was performed using Sigma Plot 14.0, and R v4.0.2.0.					
36772992	2	4	theme	METHODS	287:293	arg1	Samples					295:301	METHODS Samples	287:301	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer	287:413	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	1	5	theme	cataract	183:190	arg1	surgery					192:198	routine cataract surgery	175:198	routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification	175:284	PURPOSE To survey commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification.					
36772992	2	6	from	lots	393:396	arg1	Samples					295:301	METHODS Samples	287:301	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer	287:413	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	2	7	theme	viscoelastic	335:346	arg1	manufacturers					348:360	three different ophthalmic viscoelastic manufacturers	308:360	three different ophthalmic viscoelastic manufacturers	308:360	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	2	8	theme	different	314:322	arg1	manufacturers					348:360	three different ophthalmic viscoelastic manufacturers	308:360	three different ophthalmic viscoelastic manufacturers	308:360	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	10	9	from	differences	1554:1564	arg1	DNA					1579:1581	bacterial DNA	1569:1581	bacterial DNA	1569:1581	CONCLUSIONS No brand-specific differences in bacterial DNA were detected in the viscoelastic materials.					
36772992	3	10	theme	same	592:595	arg1	manufacturers					603:615	the same three manufacturers	588:615	the same three manufacturers	588:615	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	7	11	theme	rRNA	1115:1118	arg1	sequencing					1120:1129	16 S rRNA sequencing	1110:1129	16 S rRNA sequencing	1110:1129	16 S rRNA sequencing revealed no differences between brands in richness or overall composition.					
36772992	5	12	theme	intra-group	911:921	arg1	distances					923:931	mean intra-group distances	906:931	mean intra-group distances	906:931	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	8	13	theme	Lactobacillus	1330:1342	arg1	sp					1344:1345	Lactobacillus sp	1330:1345	Lactobacillus sp	1330:1345	The most common bacterial DNA detected across all brands was Staphylococcus sp., Cutibacterium sp., Flavobacterium sp., and Lactobacillus sp.					
36772992	3	14	theme	lysate	710:715	arg1	assay					723:727	a commercially available Limulus amebocyte lysate (LAL) assay	667:727	a commercially available Limulus amebocyte lysate (LAL) assay	667:727	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	1	15	theme	ophthalmic	129:138	arg1	materials					153:161	commonly used, sterile ophthalmic viscoelastic materials	106:161	commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification	106:284	PURPOSE To survey commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification.					
36772992	3	16	theme	Limulus	692:698	arg1	assay					723:727	a commercially available Limulus amebocyte lysate (LAL) assay	667:727	a commercially available Limulus amebocyte lysate (LAL) assay	667:727	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	12	17	theme	viscoelastic	1846:1857	arg1	samples					1859:1865	Hyvisc® viscoelastic samples	1838:1865	Hyvisc® viscoelastic samples	1838:1865	Although Anvision viscoelastic samples contained significantly more endotoxin than Hyvisc® viscoelastic samples, endotoxin concentrations were below the FDA limit of 0.2 EU/mL for both manufacturers.					
36772992	5	18	theme	sample	834:839	arg1	sites					852:856	sample collection sites	834:856	sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification	834:961	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	9	19	theme	endotoxin	1402:1410	arg1	concentration					1412:1424	the median endotoxin concentration	1391:1424	the median endotoxin concentration	1391:1424	A significant difference was found between the median endotoxin concentration between Anvision and Hyvisc® viscoelastic (Anvision: 0.171 EU/mL, Hyvisc®: 0.03 EU/mL; p < .001).					
36772992	2	20	from	manufacturers	348:360	arg1	Samples					295:301	METHODS Samples	287:301	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer	287:413	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	10	21	theme	brand-specific	1539:1552	arg1	differences					1554:1564	No brand-specific differences	1536:1564	No brand-specific differences in bacterial DNA	1536:1581	CONCLUSIONS No brand-specific differences in bacterial DNA were detected in the viscoelastic materials.					
36772992	3	22	from	manufacturers	603:615	arg1	samples					550:556	Other samples	544:556	Other samples of viscoelastic material from the same three manufacturers	544:615	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	3	22	from	manufacturers	603:615	arg1	material					574:581	viscoelastic material	561:581	viscoelastic material from the same three manufacturers	561:615	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	12	23	theme	more	1818:1821	arg1	endotoxin					1823:1831	significantly more endotoxin	1804:1831	significantly more endotoxin	1804:1831	Although Anvision viscoelastic samples contained significantly more endotoxin than Hyvisc® viscoelastic samples, endotoxin concentrations were below the FDA limit of 0.2 EU/mL for both manufacturers.					
36772992	1	24	dep	used	115:118	arg1	sterile					121:127	sterile	121:127	sterile	121:127	PURPOSE To survey commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification.					
36772992	2	25	theme	ribosomal	439:447	arg1	sequencing					473:482	16 S ribosomal ribonucleic acid (rRNA) sequencing	434:482	16 S ribosomal ribonucleic acid (rRNA) sequencing	434:482	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	0	26	theme	cataract	42:49	arg1	surgery					51:57	cataract surgery	42:57	cataract surgery	42:57	Ophthalmic viscoelastics commonly used in cataract surgery: A microbiota investigation.					
36772992	0	27	theme	Ophthalmic	0:9	arg1	viscoelastics					11:23	Ophthalmic viscoelastics	0:23	Ophthalmic viscoelastics commonly used in cataract surgery	0:57	Ophthalmic viscoelastics commonly used in cataract surgery: A microbiota investigation.					
36772992	0	28	dep	investigation	73:85	arg1	viscoelastics					11:23	Ophthalmic viscoelastics	0:23	Ophthalmic viscoelastics commonly used in cataract surgery	0:57	Ophthalmic viscoelastics commonly used in cataract surgery: A microbiota investigation.					
36772992	12	29	theme	viscoelastic	1773:1784	arg1	samples					1786:1792	Anvision viscoelastic samples	1764:1792	Anvision viscoelastic samples	1764:1792	Although Anvision viscoelastic samples contained significantly more endotoxin than Hyvisc® viscoelastic samples, endotoxin concentrations were below the FDA limit of 0.2 EU/mL for both manufacturers.					
36772992	11	30	dep	Cutibacterium	1644:1656	arg1	Staphylococcus					1628:1641	Staphylococcus, Cutibacterium, Flavobacterium, and Lactobacillus	1628:1691	Staphylococcus	1628:1641	Staphylococcus, Cutibacterium, Flavobacterium, and Lactobacillus were the dominant contributors to the bacterial DNA detected.					
36772992	13	31	theme	outcomes	2008:2015	arg1	understanding					1978:1990	the understanding	1974:1990	the understanding of inflammatory outcomes following cataract surgery	1974:2042	These data further the understanding of inflammatory outcomes following cataract surgery.					
36772992	2	32	theme	rRNA	467:470	arg1	sequencing					473:482	16 S ribosomal ribonucleic acid (rRNA) sequencing	434:482	16 S ribosomal ribonucleic acid (rRNA) sequencing	434:482	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	5	33	theme	microbial	886:894	arg1	richness					896:903	microbial richness	886:903	microbial richness	886:903	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	2	34	theme	bacterial	525:533	arg1	culture					535:541	conventional aerobic and capnophilic bacterial culture	488:541	culture	535:541	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	13	35	theme	cataract	2027:2034	arg1	surgery					2036:2042	cataract surgery	2027:2042	cataract surgery	2027:2042	These data further the understanding of inflammatory outcomes following cataract surgery.					
36772992	2	36	theme	acid	461:464	arg1	sequencing					473:482	16 S ribosomal ribonucleic acid (rRNA) sequencing	434:482	16 S ribosomal ribonucleic acid (rRNA) sequencing	434:482	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	9	37	theme	0.171	1479:1483	arg1	EU/mL					1485:1489	0.171 EU/mL	1479:1489	0.171 EU/mL	1479:1489	A significant difference was found between the median endotoxin concentration between Anvision and Hyvisc® viscoelastic (Anvision: 0.171 EU/mL, Hyvisc®: 0.03 EU/mL; p < .001).					
36772992	3	38	theme	LAL	718:720	arg1	assay					723:727	a commercially available Limulus amebocyte lysate (LAL) assay	667:727	a commercially available Limulus amebocyte lysate (LAL) assay	667:727	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	11	39	theme	bacterial	1731:1739	arg1	DNA					1741:1743	the bacterial DNA	1727:1743	the bacterial DNA detected	1727:1752	Staphylococcus, Cutibacterium, Flavobacterium, and Lactobacillus were the dominant contributors to the bacterial DNA detected.					
36772992	3	40	theme	Other	544:548	arg1	samples					550:556	Other samples	544:556	Other samples of viscoelastic material from the same three manufacturers	544:615	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	5	41	theme	total	861:865	arg1	concentration					871:883	total DNA concentration	861:883	total DNA concentration	861:883	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	9	42	dep	Anvision	1434:1441	arg1	Anvision					1469:1476	Anvision	1469:1476	Anvision	1469:1476	A significant difference was found between the median endotoxin concentration between Anvision and Hyvisc® viscoelastic (Anvision: 0.171 EU/mL, Hyvisc®: 0.03 EU/mL; p < .001).					
36772992	9	42	dep	Anvision	1434:1441	arg1	Hyvisc®					1492:1498	Hyvisc®	1492:1498	Hyvisc®	1492:1498	A significant difference was found between the median endotoxin concentration between Anvision and Hyvisc® viscoelastic (Anvision: 0.171 EU/mL, Hyvisc®: 0.03 EU/mL; p < .001).					
36772992	1	43	theme	DNA	230:232	arg1	presence					208:215	the presence	204:215	the presence of bacterial DNA and/or viable bacteria and endotoxin quantification	204:284	PURPOSE To survey commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification.					
36772992	8	44	theme	bacterial	1222:1230	arg1	sp.					1282:1284	Staphylococcus sp.	1267:1284	Staphylococcus sp.	1267:1284	The most common bacterial DNA detected across all brands was Staphylococcus sp., Cutibacterium sp., Flavobacterium sp., and Lactobacillus sp.					
36772992	8	44	theme	bacterial	1222:1230	arg1	DNA					1232:1234	The most common bacterial DNA	1206:1234	The most common bacterial DNA detected across all brands	1206:1261	The most common bacterial DNA detected across all brands was Staphylococcus sp., Cutibacterium sp., Flavobacterium sp., and Lactobacillus sp.					
36772992	3	45	theme	material	574:581	arg1	samples					550:556	Other samples	544:556	Other samples of viscoelastic material from the same three manufacturers	544:615	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	1	46	theme	viable	241:246	arg1	bacteria					248:255	viable bacteria	241:255	viable bacteria	241:255	PURPOSE To survey commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification.					
36772992	6	47	theme	RESULTS	1006:1012	arg1	Culture					1014:1020	RESULTS Culture	1006:1020	RESULTS Culture	1006:1020	RESULTS Culture yielded two isolates, identified as Staphylococcus epidermidis and Bacillus megaterium.					
36772992	12	48	contain	contained	1794:1802	arg1	samples					1786:1792	Anvision viscoelastic samples	1764:1792	Anvision viscoelastic samples	1764:1792	Although Anvision viscoelastic samples contained significantly more endotoxin than Hyvisc® viscoelastic samples, endotoxin concentrations were below the FDA limit of 0.2 EU/mL for both manufacturers.					
36772992	12	48	contain	contained	1794:1802	arg2	endotoxin					1823:1831	significantly more endotoxin	1804:1831	significantly more endotoxin	1804:1831	Although Anvision viscoelastic samples contained significantly more endotoxin than Hyvisc® viscoelastic samples, endotoxin concentrations were below the FDA limit of 0.2 EU/mL for both manufacturers.					
36772992	12	49	theme	EU/mL	1925:1929	arg1	limit					1912:1916	the FDA limit	1904:1916	the FDA limit of 0.2 EU/mL for both manufacturers	1904:1952	Although Anvision viscoelastic samples contained significantly more endotoxin than Hyvisc® viscoelastic samples, endotoxin concentrations were below the FDA limit of 0.2 EU/mL for both manufacturers.					
36772992	1	50	theme	quantification	271:284	arg1	presence					208:215	the presence	204:215	the presence of bacterial DNA and/or viable bacteria and endotoxin quantification	204:284	PURPOSE To survey commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification.					
36772992	5	51	dep	concentration	871:883	arg1	quantification					948:961	quantification	948:961	quantification	948:961	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	2	52	theme	production	382:391	arg1	lots					393:396	three different production lots	366:396	three different production lots per manufacturer	366:413	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	1	53	theme	routine	175:181	arg1	surgery					192:198	routine cataract surgery	175:198	routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification	175:284	PURPOSE To survey commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification.					
36772992	12	54	theme	FDA	1908:1910	arg1	limit					1912:1916	the FDA limit	1904:1916	the FDA limit of 0.2 EU/mL for both manufacturers	1904:1952	Although Anvision viscoelastic samples contained significantly more endotoxin than Hyvisc® viscoelastic samples, endotoxin concentrations were below the FDA limit of 0.2 EU/mL for both manufacturers.					
36772992	10	55	located	detected	1588:1595	arg1	materials					1617:1625	the viscoelastic materials	1600:1625	the viscoelastic materials	1600:1625	CONCLUSIONS No brand-specific differences in bacterial DNA were detected in the viscoelastic materials.					
36772992	10	55	located	detected	1588:1595	arg2	differences					1554:1564	No brand-specific differences	1536:1564	No brand-specific differences in bacterial DNA	1536:1581	CONCLUSIONS No brand-specific differences in bacterial DNA were detected in the viscoelastic materials.					
36772992	7	56	theme	overall	1185:1191	arg1	composition					1193:1203	overall composition	1185:1203	overall composition	1185:1203	16 S rRNA sequencing revealed no differences between brands in richness or overall composition.					
36772992	11	57	theme	dominant	1702:1709	arg1	contributors					1711:1722	the dominant contributors	1698:1722	the dominant contributors to the bacterial DNA detected	1698:1752	Staphylococcus, Cutibacterium, Flavobacterium, and Lactobacillus were the dominant contributors to the bacterial DNA detected.					
36772992	11	57	theme	dominant	1702:1709	arg1	Flavobacterium					1659:1672	Flavobacterium	1659:1672	Flavobacterium	1659:1672	Staphylococcus, Cutibacterium, Flavobacterium, and Lactobacillus were the dominant contributors to the bacterial DNA detected.					
36772992	11	57	theme	dominant	1702:1709	arg1	Lactobacillus					1679:1691	Lactobacillus	1679:1691	Lactobacillus	1679:1691	Staphylococcus, Cutibacterium, Flavobacterium, and Lactobacillus were the dominant contributors to the bacterial DNA detected.					
36772992	11	57	theme	dominant	1702:1709	arg1	Cutibacterium					1644:1656	Cutibacterium	1644:1656	Cutibacterium	1644:1656	Staphylococcus, Cutibacterium, Flavobacterium, and Lactobacillus were the dominant contributors to the bacterial DNA detected.					
36772992	3	58	from	samples	550:556	arg1	manufacturers					603:615	the same three manufacturers	588:615	the same three manufacturers	588:615	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	2	59	theme	ophthalmic	324:333	arg1	manufacturers					348:360	three different ophthalmic viscoelastic manufacturers	308:360	three different ophthalmic viscoelastic manufacturers	308:360	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	3	60	theme	endotoxin	636:644	arg1	quantification					646:659	endotoxin quantification	636:659	endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay	636:727	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	5	61	theme	mean	906:909	arg1	distances					923:931	mean intra-group distances	906:931	mean intra-group distances	906:931	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	9	62	theme	significant	1350:1360	arg1	difference					1362:1371	A significant difference	1348:1371	A significant difference	1348:1371	A significant difference was found between the median endotoxin concentration between Anvision and Hyvisc® viscoelastic (Anvision: 0.171 EU/mL, Hyvisc®: 0.03 EU/mL; p < .001).					
36772992	1	63	theme	used	115:118	arg1	materials					153:161	commonly used, sterile ophthalmic viscoelastic materials	106:161	commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification	106:284	PURPOSE To survey commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification.					
36772992	3	64	theme	amebocyte	700:708	arg1	assay					723:727	a commercially available Limulus amebocyte lysate (LAL) assay	667:727	a commercially available Limulus amebocyte lysate (LAL) assay	667:727	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	7	65	theme	16 S	1110:1113	arg1	sequencing					1120:1129	16 S rRNA sequencing	1110:1129	16 S rRNA sequencing	1110:1129	16 S rRNA sequencing revealed no differences between brands in richness or overall composition.					
36772992	12	66	theme	endotoxin	1868:1876	arg1	concentrations					1878:1891	endotoxin concentrations	1868:1891	endotoxin concentrations	1868:1891	Although Anvision viscoelastic samples contained significantly more endotoxin than Hyvisc® viscoelastic samples, endotoxin concentrations were below the FDA limit of 0.2 EU/mL for both manufacturers.					
36772992	0	67	theme	microbiota	62:71	arg1	investigation					73:85	A microbiota investigation	60:85	A microbiota investigation	60:85	Ophthalmic viscoelastics commonly used in cataract surgery: A microbiota investigation.					
36772992	3	68	theme	available	682:690	arg1	assay					723:727	a commercially available Limulus amebocyte lysate (LAL) assay	667:727	a commercially available Limulus amebocyte lysate (LAL) assay	667:727	Other samples of viscoelastic material from the same three manufacturers were collected for endotoxin quantification using a commercially available Limulus amebocyte lysate (LAL) assay.					
36772992	1	69	theme	viscoelastic	140:151	arg1	materials					153:161	commonly used, sterile ophthalmic viscoelastic materials	106:161	commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification	106:284	PURPOSE To survey commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification.					
36772992	9	70	dep	Anvision	1469:1476	arg1	EU/mL					1506:1510	0.03 EU/mL	1501:1510	0.03 EU/mL	1501:1510	A significant difference was found between the median endotoxin concentration between Anvision and Hyvisc® viscoelastic (Anvision: 0.171 EU/mL, Hyvisc®: 0.03 EU/mL; p < .001).					
36772992	9	70	dep	Anvision	1469:1476	arg1	EU/mL					1485:1489	0.171 EU/mL	1479:1489	0.171 EU/mL	1479:1489	A significant difference was found between the median endotoxin concentration between Anvision and Hyvisc® viscoelastic (Anvision: 0.171 EU/mL, Hyvisc®: 0.03 EU/mL; p < .001).					
36772992	9	70	dep	Anvision	1469:1476	arg1	p < .001					1513:1520	p < .001	1513:1520	p < .001	1513:1520	A significant difference was found between the median endotoxin concentration between Anvision and Hyvisc® viscoelastic (Anvision: 0.171 EU/mL, Hyvisc®: 0.03 EU/mL; p < .001).					
36772992	9	71	theme	median	1395:1400	arg1	concentration					1412:1424	the median endotoxin concentration	1391:1424	the median endotoxin concentration	1391:1424	A significant difference was found between the median endotoxin concentration between Anvision and Hyvisc® viscoelastic (Anvision: 0.171 EU/mL, Hyvisc®: 0.03 EU/mL; p < .001).					
36772992	5	72	theme	reagent	973:979	arg1	controls					981:988	reagent controls	973:988	reagent controls	973:988	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	12	73	theme	Hyvisc®	1838:1844	arg1	samples					1859:1865	Hyvisc® viscoelastic samples	1838:1865	Hyvisc® viscoelastic samples	1838:1865	Although Anvision viscoelastic samples contained significantly more endotoxin than Hyvisc® viscoelastic samples, endotoxin concentrations were below the FDA limit of 0.2 EU/mL for both manufacturers.					
36772992	4	74	theme	Sigma	771:775	arg1	Plot					777:780	Sigma Plot 14.0	771:785	Sigma Plot 14.0	771:785	Statistical analysis was performed using Sigma Plot 14.0, and R v4.0.2.0.					
36772992	5	75	from	sites	852:856	arg1	concentration					871:883	total DNA concentration	861:883	total DNA concentration	861:883	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	5	75	from	sites	852:856	arg1	richness					896:903	microbial richness	886:903	microbial richness	886:903	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	5	75	from	sites	852:856	arg1	distances					923:931	mean intra-group distances	906:931	mean intra-group distances	906:931	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	5	75	from	sites	852:856	arg1	endotoxin					938:946	endotoxin	938:946	endotoxin	938:946	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	2	76	theme	16 S	434:437	arg1	sequencing					473:482	16 S ribosomal ribonucleic acid (rRNA) sequencing	434:482	16 S ribosomal ribonucleic acid (rRNA) sequencing	434:482	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	5	77	theme	DNA	867:869	arg1	concentration					871:883	total DNA concentration	861:883	total DNA concentration	861:883	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	10	78	theme	bacterial	1569:1577	arg1	DNA					1579:1581	bacterial DNA	1569:1581	bacterial DNA	1569:1581	CONCLUSIONS No brand-specific differences in bacterial DNA were detected in the viscoelastic materials.					
36772992	13	79	theme	inflammatory	1995:2006	arg1	outcomes					2008:2015	inflammatory outcomes	1995:2015	inflammatory outcomes following cataract surgery	1995:2042	These data further the understanding of inflammatory outcomes following cataract surgery.					
36772992	12	80	theme	Anvision	1764:1771	arg1	samples					1786:1792	Anvision viscoelastic samples	1764:1792	Anvision viscoelastic samples	1764:1792	Although Anvision viscoelastic samples contained significantly more endotoxin than Hyvisc® viscoelastic samples, endotoxin concentrations were below the FDA limit of 0.2 EU/mL for both manufacturers.					
36772992	10	81	theme	viscoelastic	1604:1615	arg1	materials					1617:1625	the viscoelastic materials	1600:1625	the viscoelastic materials	1600:1625	CONCLUSIONS No brand-specific differences in bacterial DNA were detected in the viscoelastic materials.					
36772992	5	82	theme	collection	841:850	arg1	sites					852:856	sample collection sites	834:856	sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification	834:961	Differences (p ≤ .05) between sample collection sites in total DNA concentration, microbial richness, mean intra-group distances, and endotoxin quantification alongside reagent controls were evaluated.					
36772992	2	83	theme	capnophilic	513:523	arg1	culture					535:541	conventional aerobic and capnophilic bacterial culture	488:541	culture	535:541	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	2	84	theme	ribonucleic	449:459	arg1	sequencing					473:482	16 S ribosomal ribonucleic acid (rRNA) sequencing	434:482	16 S ribosomal ribonucleic acid (rRNA) sequencing	434:482	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	8	85	theme	common	1215:1220	arg1	sp.					1282:1284	Staphylococcus sp.	1267:1284	Staphylococcus sp.	1267:1284	The most common bacterial DNA detected across all brands was Staphylococcus sp., Cutibacterium sp., Flavobacterium sp., and Lactobacillus sp.					
36772992	8	85	theme	common	1215:1220	arg1	DNA					1232:1234	The most common bacterial DNA	1206:1234	The most common bacterial DNA detected across all brands	1206:1261	The most common bacterial DNA detected across all brands was Staphylococcus sp., Cutibacterium sp., Flavobacterium sp., and Lactobacillus sp.					
36772992	2	86	theme	different	372:380	arg1	lots					393:396	three different production lots	366:396	three different production lots per manufacturer	366:413	METHODS Samples from three different ophthalmic viscoelastic manufacturers and three different production lots per manufacturer were collected for 16 S ribosomal ribonucleic acid (rRNA) sequencing and conventional aerobic and capnophilic bacterial culture.					
36772992	10	87	dep	CONCLUSIONS	1524:1534	arg1	detected					1588:1595	detected	1588:1595	were detected in the viscoelastic materials	1583:1625	CONCLUSIONS No brand-specific differences in bacterial DNA were detected in the viscoelastic materials.					
36772992	1	88	theme	bacterial	220:228	arg1	DNA					230:232	bacterial DNA	220:232	bacterial DNA	220:232	PURPOSE To survey commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification.					
36772992	1	89	theme	bacteria	248:255	arg1	quantification					271:284	viable bacteria and endotoxin quantification	241:284	viable bacteria and endotoxin quantification	241:284	PURPOSE To survey commonly used, sterile ophthalmic viscoelastic materials used during routine cataract surgery for the presence of bacterial DNA and/or viable bacteria and endotoxin quantification.					
37006776	6	0	theme	NMR	926:928	arg1	approach					930:937	a new NMR approach	920:937	a new NMR approach to perform competition experiments between glycans with similar compositions and different lengths	920:1036	In addition, we developed a new NMR approach to perform competition experiments between glycans with similar compositions and different lengths.					
37006776	4	1	theme	pandemic	712:719	arg1	outbreak					721:728	the 2009 pandemic outbreak	703:728	the 2009 pandemic outbreak	703:728	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	5	2	theme	H6N1	763:766	arg1	mutant					768:773	one engineered H6N1 mutant	748:773	one engineered H6N1 mutant	748:773	We also analyzed one engineered H6N1 mutant to understand if the preference for tri-LacNAc motifs could be a general trend in human-type receptor-adapted viruses.					
37006776	0	3	theme	Complex	61:67	arg1	N-Glycans					69:77	Complex N-Glycans	61:77	Complex N-Glycans	61:77	Revealing the Specificity of Human H1 Influenza A Viruses to Complex N-Glycans.					
37006776	6	4	theme	new	922:924	arg1	approach					930:937	a new NMR approach	920:937	a new NMR approach to perform competition experiments between glycans with similar compositions and different lengths	920:1036	In addition, we developed a new NMR approach to perform competition experiments between glycans with similar compositions and different lengths.					
37006776	1	5	theme	viral	145:149	arg1	hemagglutinins					151:164	viral hemagglutinins	145:164	viral hemagglutinins	145:164	Influenza virus infection remains a threat to human health since viral hemagglutinins are constantly drifting, escaping infection and vaccine-induced antibody responses.					
37006776	6	6	theme	different	1020:1028	arg1	lengths					1030:1036	different lengths	1020:1036	different lengths	1020:1036	In addition, we developed a new NMR approach to perform competition experiments between glycans with similar compositions and different lengths.					
37006776	6	7	with	glycans	982:988	arg1	compositions					1003:1014	similar compositions	995:1014	similar compositions	995:1014	In addition, we developed a new NMR approach to perform competition experiments between glycans with similar compositions and different lengths.					
37006776	6	7	with	glycans	982:988	arg1	lengths					1030:1036	different lengths	1020:1036	different lengths	1020:1036	In addition, we developed a new NMR approach to perform competition experiments between glycans with similar compositions and different lengths.					
37006776	4	8	theme	glycan	622:627	arg1	specificity					629:639	the glycan specificity	618:639	the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak	618:728	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	7	9	theme	strict	1132:1137	arg1	preference					1139:1148	a strict preference	1130:1148	a strict preference for a minimum of di-LacNAc structural motifs	1130:1193	Our results point out that pandemic H1 viruses differ from previous seasonal H1 viruses by a strict preference for a minimum of di-LacNAc structural motifs.					
37006776	3	10	theme	H3N2	361:364	arg1	viruses					366:372	recent H3N2 viruses	354:372	recent H3N2 viruses	354:372	In this context, recent H3N2 viruses have specificity for α2,6 sialylated branched N-glycans with at least three N-acetyllactosamine units (tri-LacNAc).					
37006776	5	11	theme	receptor-adapted	868:883	arg1	viruses					885:891	human-type receptor-adapted viruses	857:891	human-type receptor-adapted viruses	857:891	We also analyzed one engineered H6N1 mutant to understand if the preference for tri-LacNAc motifs could be a general trend in human-type receptor-adapted viruses.					
37006776	5	12	theme	tri-LacNAc	811:820	arg1	motifs					822:827	tri-LacNAc motifs	811:827	tri-LacNAc motifs	811:827	We also analyzed one engineered H6N1 mutant to understand if the preference for tri-LacNAc motifs could be a general trend in human-type receptor-adapted viruses.					
37006776	3	13	gly	sialylated	400:409	arg1	N-glycans					420:428	sialylated branched N-glycans	400:428	sialylated branched N-glycans	400:428	In this context, recent H3N2 viruses have specificity for α2,6 sialylated branched N-glycans with at least three N-acetyllactosamine units (tri-LacNAc).					
37006776	5	14	theme	engineered	752:761	arg1	mutant					768:773	one engineered H6N1 mutant	748:773	one engineered H6N1 mutant	748:773	We also analyzed one engineered H6N1 mutant to understand if the preference for tri-LacNAc motifs could be a general trend in human-type receptor-adapted viruses.					
37006776	3	15	theme	sialylated	400:409	arg1	N-glycans					420:428	sialylated branched N-glycans	400:428	sialylated branched N-glycans	400:428	In this context, recent H3N2 viruses have specificity for α2,6 sialylated branched N-glycans with at least three N-acetyllactosamine units (tri-LacNAc).					
37006776	7	16	theme	seasonal	1107:1114	arg1	viruses					1119:1125	previous seasonal H1 viruses	1098:1125	previous seasonal H1 viruses	1098:1125	Our results point out that pandemic H1 viruses differ from previous seasonal H1 viruses by a strict preference for a minimum of di-LacNAc structural motifs.					
37006776	6	17	theme	similar	995:1001	arg1	compositions					1003:1014	similar compositions	995:1014	similar compositions	995:1014	In addition, we developed a new NMR approach to perform competition experiments between glycans with similar compositions and different lengths.					
37006776	7	18	dep	point	1051:1055	arg1	out					1057:1059	out	1057:1059	out	1057:1059	Our results point out that pandemic H1 viruses differ from previous seasonal H1 viruses by a strict preference for a minimum of di-LacNAc structural motifs.					
37006776	4	19	theme	responsible	687:697	arg1	one					683:685	the one	679:685	the one responsible for the 2009 pandemic outbreak	679:728	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	3	20	theme	branched	411:418	arg1	N-glycans					420:428	sialylated branched N-glycans	400:428	sialylated branched N-glycans	400:428	In this context, recent H3N2 viruses have specificity for α2,6 sialylated branched N-glycans with at least three N-acetyllactosamine units (tri-LacNAc).					
37006776	4	21	theme	magnetic	571:578	arg1	resonance					580:588	nuclear magnetic resonance	563:588	nuclear magnetic resonance experiments	563:600	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	7	22	theme	H1	1116:1117	arg1	viruses					1119:1125	previous seasonal H1 viruses	1098:1125	previous seasonal H1 viruses	1098:1125	Our results point out that pandemic H1 viruses differ from previous seasonal H1 viruses by a strict preference for a minimum of di-LacNAc structural motifs.					
37006776	1	23	theme	Influenza	80:88	arg1	infection					96:104	Influenza virus infection	80:104	Influenza virus infection	80:104	Influenza virus infection remains a threat to human health since viral hemagglutinins are constantly drifting, escaping infection and vaccine-induced antibody responses.					
37006776	3	24	theme	N-acetyllactosamine	450:468	arg1	units					470:474	at least three N-acetyllactosamine units	435:474	at least three N-acetyllactosamine units (tri-LacNAc)	435:487	In this context, recent H3N2 viruses have specificity for α2,6 sialylated branched N-glycans with at least three N-acetyllactosamine units (tri-LacNAc).					
37006776	3	24	theme	N-acetyllactosamine	450:468	arg1	tri-LacNAc					477:486	tri-LacNAc	477:486	tri-LacNAc	477:486	In this context, recent H3N2 viruses have specificity for α2,6 sialylated branched N-glycans with at least three N-acetyllactosamine units (tri-LacNAc).					
37006776	7	25	theme	structural	1177:1186	arg1	motifs					1188:1193	di-LacNAc structural motifs	1167:1193	di-LacNAc structural motifs	1167:1193	Our results point out that pandemic H1 viruses differ from previous seasonal H1 viruses by a strict preference for a minimum of di-LacNAc structural motifs.					
37006776	4	26	theme	nuclear	563:569	arg1	resonance					580:588	nuclear magnetic resonance	563:588	nuclear magnetic resonance experiments	563:600	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	1	27	theme	virus	90:94	arg1	infection					96:104	Influenza virus infection	80:104	Influenza virus infection	80:104	Influenza virus infection remains a threat to human health since viral hemagglutinins are constantly drifting, escaping infection and vaccine-induced antibody responses.					
37006776	4	28	theme	H1	656:657	arg1	variants					659:666	H1 variants	656:666	H1 variants	656:666	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	4	29	with	analyses	549:556	arg1	experiments					590:600	nuclear magnetic resonance experiments	563:600	nuclear magnetic resonance experiments	563:600	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	7	30	theme	previous	1098:1105	arg1	viruses					1119:1125	previous seasonal H1 viruses	1098:1125	previous seasonal H1 viruses	1098:1125	Our results point out that pandemic H1 viruses differ from previous seasonal H1 viruses by a strict preference for a minimum of di-LacNAc structural motifs.					
37006776	2	31	theme	glycan	317:322	arg1	recognition					324:334	glycan recognition	317:334	glycan recognition	317:334	Viral hemagglutinins from different viruses display variability in glycan recognition.					
37006776	0	32	theme	H1	35:36	arg1	Viruses					50:56	Human H1 Influenza A Viruses	29:56	Human H1 Influenza A Viruses	29:56	Revealing the Specificity of Human H1 Influenza A Viruses to Complex N-Glycans.					
37006776	4	33	theme	resonance	580:588	arg1	experiments					590:600	nuclear magnetic resonance experiments	563:600	nuclear magnetic resonance experiments	563:600	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	0	34	theme	Human	29:33	arg1	Viruses					50:56	Human H1 Influenza A Viruses	29:56	Human H1 Influenza A Viruses	29:56	Revealing the Specificity of Human H1 Influenza A Viruses to Complex N-Glycans.					
37006776	4	35	theme	binding	541:547	arg1	analyses					549:556	tissue binding analyses	534:556	tissue binding analyses	534:556	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	7	36	theme	di-LacNAc	1167:1175	arg1	motifs					1188:1193	di-LacNAc structural motifs	1167:1193	di-LacNAc structural motifs	1167:1193	Our results point out that pandemic H1 viruses differ from previous seasonal H1 viruses by a strict preference for a minimum of di-LacNAc structural motifs.					
37006776	4	37	theme	variants	659:666	arg1	family					646:651	a family	644:651	a family	644:651	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	0	38	theme	A	48:48	arg1	Viruses					50:56	Human H1 Influenza A Viruses	29:56	Human H1 Influenza A Viruses	29:56	Revealing the Specificity of Human H1 Influenza A Viruses to Complex N-Glycans.					
37006776	4	39	theme	tissue	534:539	arg1	analyses					549:556	tissue binding analyses	534:556	tissue binding analyses	534:556	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	7	40	theme	pandemic	1066:1073	arg1	viruses					1078:1084	pandemic H1 viruses	1066:1084	pandemic H1 viruses	1066:1084	Our results point out that pandemic H1 viruses differ from previous seasonal H1 viruses by a strict preference for a minimum of di-LacNAc structural motifs.					
37006776	3	41	theme	recent	354:359	arg1	viruses					366:372	recent H3N2 viruses	354:372	recent H3N2 viruses	354:372	In this context, recent H3N2 viruses have specificity for α2,6 sialylated branched N-glycans with at least three N-acetyllactosamine units (tri-LacNAc).					
37006776	0	42	theme	Influenza	38:46	arg1	Viruses					50:56	Human H1 Influenza A Viruses	29:56	Human H1 Influenza A Viruses	29:56	Revealing the Specificity of Human H1 Influenza A Viruses to Complex N-Glycans.					
37006776	7	43	theme	H1	1075:1076	arg1	viruses					1078:1084	pandemic H1 viruses	1066:1084	pandemic H1 viruses	1066:1084	Our results point out that pandemic H1 viruses differ from previous seasonal H1 viruses by a strict preference for a minimum of di-LacNAc structural motifs.					
37006776	4	44	with	arrays	523:528	arg1	experiments					590:600	nuclear magnetic resonance experiments	563:600	nuclear magnetic resonance experiments	563:600	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	2	45	theme	different	276:284	arg1	viruses					286:292	different viruses	276:292	different viruses	276:292	Viral hemagglutinins from different viruses display variability in glycan recognition.					
37006776	3	46	contain	have	374:377	arg2	specificity					379:389	specificity	379:389	specificity for α2,6 sialylated branched N-glycans with at least three N-acetyllactosamine units (tri-LacNAc)	379:487	In this context, recent H3N2 viruses have specificity for α2,6 sialylated branched N-glycans with at least three N-acetyllactosamine units (tri-LacNAc).					
37006776	3	46	contain	have	374:377	arg1	viruses					366:372	recent H3N2 viruses	354:372	recent H3N2 viruses	354:372	In this context, recent H3N2 viruses have specificity for α2,6 sialylated branched N-glycans with at least three N-acetyllactosamine units (tri-LacNAc).					
37006776	2	47	from	viruses	286:292	arg1	hemagglutinins					256:269	Viral hemagglutinins	250:269	Viral hemagglutinins from different viruses	250:292	Viral hemagglutinins from different viruses display variability in glycan recognition.					
37006776	1	48	theme	vaccine-induced	214:228	arg1	responses					239:247	vaccine-induced antibody responses	214:247	vaccine-induced antibody responses	214:247	Influenza virus infection remains a threat to human health since viral hemagglutinins are constantly drifting, escaping infection and vaccine-induced antibody responses.					
37006776	5	49	from	trend	848:852	arg1	viruses					885:891	human-type receptor-adapted viruses	857:891	human-type receptor-adapted viruses	857:891	We also analyzed one engineered H6N1 mutant to understand if the preference for tri-LacNAc motifs could be a general trend in human-type receptor-adapted viruses.					
37006776	1	50	theme	antibody	230:237	arg1	responses					239:247	vaccine-induced antibody responses	214:247	vaccine-induced antibody responses	214:247	Influenza virus infection remains a threat to human health since viral hemagglutinins are constantly drifting, escaping infection and vaccine-induced antibody responses.					
37006776	5	51	theme	human-type	857:866	arg1	viruses					885:891	human-type receptor-adapted viruses	857:891	human-type receptor-adapted viruses	857:891	We also analyzed one engineered H6N1 mutant to understand if the preference for tri-LacNAc motifs could be a general trend in human-type receptor-adapted viruses.					
37006776	5	52	theme	general	840:846	arg1	trend					848:852	a general trend	838:852	a general trend in human-type receptor-adapted viruses	838:891	We also analyzed one engineered H6N1 mutant to understand if the preference for tri-LacNAc motifs could be a general trend in human-type receptor-adapted viruses.					
37006776	5	52	theme	general	840:846	arg1	preference					796:805	the preference	792:805	the preference for tri-LacNAc motifs	792:827	We also analyzed one engineered H6N1 mutant to understand if the preference for tri-LacNAc motifs could be a general trend in human-type receptor-adapted viruses.					
37006776	1	53	theme	human	126:130	arg1	health					132:137	human health	126:137	human health	126:137	Influenza virus infection remains a threat to human health since viral hemagglutinins are constantly drifting, escaping infection and vaccine-induced antibody responses.					
37006776	4	54	theme	family	646:651	arg1	specificity					629:639	the glycan specificity	618:639	the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak	618:728	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	0	55	theme	Viruses	50:56	arg1	Specificity					14:24	the Specificity	10:24	the Specificity of Human H1 Influenza A Viruses to Complex N-Glycans	10:77	Revealing the Specificity of Human H1 Influenza A Viruses to Complex N-Glycans.					
37006776	4	56	theme	glycan	516:521	arg1	arrays					523:528	glycan arrays	516:528	glycan arrays	516:528	In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak.					
37006776	2	57	theme	Viral	250:254	arg1	hemagglutinins					256:269	Viral hemagglutinins	250:269	Viral hemagglutinins from different viruses	250:292	Viral hemagglutinins from different viruses display variability in glycan recognition.					
37006776	7	58	theme	motifs	1188:1193	arg1	minimum					1156:1162	a minimum	1154:1162	a minimum of di-LacNAc structural motifs	1154:1193	Our results point out that pandemic H1 viruses differ from previous seasonal H1 viruses by a strict preference for a minimum of di-LacNAc structural motifs.					
37006776	6	59	theme	competition	950:960	arg1	experiments					962:972	competition experiments	950:972	competition experiments between glycans with similar compositions and different lengths	950:1036	In addition, we developed a new NMR approach to perform competition experiments between glycans with similar compositions and different lengths.					
36750373	6	0	theme	defective	1242:1250	arg1	JIUYAO					1252:1257	Type I defective JIUYAO	1235:1257	Type I defective JIUYAO	1235:1257	Type I defective JIUYAO contained a higher abundance of aerobic Pediococcus and Marivita compared to the normal JIUYAO.					
36750373	7	1	theme	higher	1395:1400	arg1	abundance					1402:1410	a higher abundance	1393:1410	a higher abundance of anaerobic Mucor and Staphylococcus	1393:1448	Type II defective JIUYAO consisted of a higher abundance of anaerobic Mucor and Staphylococcus.					
36750373	6	2	theme	Type	1235:1238	arg1	JIUYAO					1252:1257	Type I defective JIUYAO	1235:1257	Type I defective JIUYAO	1235:1257	Type I defective JIUYAO contained a higher abundance of aerobic Pediococcus and Marivita compared to the normal JIUYAO.					
36750373	3	3	theme	RESULTS	527:533	arg1	Water					535:539	RESULTS Water and starch contents	527:559	Water	535:539	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	7	4	theme	anaerobic	1415:1423	arg1	Mucor					1425:1429	anaerobic Mucor	1415:1429	anaerobic Mucor	1415:1429	Type II defective JIUYAO consisted of a higher abundance of anaerobic Mucor and Staphylococcus.					
36750373	3	5	theme	protein	713:719	arg1	activity					799:806	neutral protease activity	782:806	neutral protease activity	782:806	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	5	theme	protein	713:719	arg1	contents					721:728	the protein contents	709:728	the protein contents	709:728	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	5	theme	protein	713:719	arg1	capability					744:753	liquefaction capability	731:753	liquefaction capability	731:753	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	5	theme	protein	713:719	arg1	capability					766:775	glycation capability	756:775	glycation capability	756:775	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	5	theme	protein	713:719	arg1	higher					827:832	higher	827:832	higher	827:832	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	1	6	theme	traditional	178:188	arg1	brewing					199:205	traditional Huangjiu brewing	178:205	traditional Huangjiu brewing	178:205	BACKGROUND JIUYAO is a critical fermenting agent in traditional Huangjiu brewing and affects the quality of Huangjiu.					
36750373	6	7	theme	Pediococcus	1299:1309	arg1	abundance					1278:1286	a higher abundance	1269:1286	a higher abundance of aerobic Pediococcus and Marivita compared to the normal JIUYAO	1269:1352	Type I defective JIUYAO contained a higher abundance of aerobic Pediococcus and Marivita compared to the normal JIUYAO.					
36750373	2	8	theme	JIUYAO	281:286	arg1	quality					270:276	the quality	266:276	the quality of JIUYAO	266:286	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	4	9	theme	lower	910:914	arg1	indices					916:922	significantly lower indices	896:922	significantly lower indices	896:922	Type II defective JIUYAO had significantly lower indices than the normal group except for starch and free amino acid contents, which were significantly higher than those of the normal JIUYAO.					
36750373	3	10	theme	esterification	594:607	arg1	capability					609:618	the esterification capability	590:618	the esterification capability of type I defective JIUYAO	590:645	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	10	theme	esterification	594:607	arg1	lower					666:670	lower	666:670	lower	666:670	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	4	11	theme	Type	867:870	arg1	JIUYAO					885:890	Type II defective JIUYAO	867:890	Type II defective JIUYAO	867:890	Type II defective JIUYAO had significantly lower indices than the normal group except for starch and free amino acid contents, which were significantly higher than those of the normal JIUYAO.					
36750373	6	12	theme	higher	1271:1276	arg1	abundance					1278:1286	a higher abundance	1269:1286	a higher abundance of aerobic Pediococcus and Marivita compared to the normal JIUYAO	1269:1352	Type I defective JIUYAO contained a higher abundance of aerobic Pediococcus and Marivita compared to the normal JIUYAO.					
36750373	3	13	theme	acid	562:565	arg1	activity					576:583	acid protease activity	562:583	acid protease activity	562:583	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	4	14	theme	defective	875:883	arg1	JIUYAO					885:890	Type II defective JIUYAO	867:890	Type II defective JIUYAO	867:890	Type II defective JIUYAO had significantly lower indices than the normal group except for starch and free amino acid contents, which were significantly higher than those of the normal JIUYAO.					
36750373	10	15	theme	JIUYAO	1788:1793	arg1	evaluation					1762:1771	the quality evaluation and control	1750:1783	evaluation	1762:1771	We believe that the present study offers a theoretical basis for the quality evaluation and control of JIUYAO.					
36750373	10	15	theme	JIUYAO	1788:1793	arg1	control					1777:1783	the quality evaluation and control	1750:1783	control	1777:1783	We believe that the present study offers a theoretical basis for the quality evaluation and control of JIUYAO.					
36750373	1	16	theme	BACKGROUND	126:135	arg1	agent					169:173	a critical fermenting agent	147:173	a critical fermenting agent in traditional Huangjiu brewing	147:205	BACKGROUND JIUYAO is a critical fermenting agent in traditional Huangjiu brewing and affects the quality of Huangjiu.					
36750373	1	16	theme	BACKGROUND	126:135	arg1	JIUYAO					137:142	BACKGROUND JIUYAO	126:142	BACKGROUND JIUYAO	126:142	BACKGROUND JIUYAO is a critical fermenting agent in traditional Huangjiu brewing and affects the quality of Huangjiu.					
36750373	2	17	theme	volatile	471:478	arg1	components					439:448	the main components	430:448	the main components	430:448	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	2	17	theme	volatile	471:478	arg1	substances					480:489	volatile substances	471:489	volatile substances	471:489	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	3	18	theme	glycation	756:764	arg1	contents					721:728	the protein contents	709:728	the protein contents	709:728	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	18	theme	glycation	756:764	arg1	capability					766:775	glycation capability	756:775	glycation capability	756:775	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	0	19	theme	enzyme	45:50	arg1	activities					52:61	enzyme activities	45:61	enzyme activities	45:61	Study on the differences of main components, enzyme activities and microbial composition between defective and normal JIUYAO.					
36750373	6	20	contain	contained	1259:1267	arg2	abundance					1278:1286	a higher abundance	1269:1286	a higher abundance of aerobic Pediococcus and Marivita compared to the normal JIUYAO	1269:1352	Type I defective JIUYAO contained a higher abundance of aerobic Pediococcus and Marivita compared to the normal JIUYAO.					
36750373	6	20	contain	contained	1259:1267	arg1	JIUYAO					1252:1257	Type I defective JIUYAO	1235:1257	Type I defective JIUYAO	1235:1257	Type I defective JIUYAO contained a higher abundance of aerobic Pediococcus and Marivita compared to the normal JIUYAO.					
36750373	4	21	theme	starch	957:962	arg1	contents					984:991	starch and free amino acid contents	957:991	starch and free amino acid contents	957:991	Type II defective JIUYAO had significantly lower indices than the normal group except for starch and free amino acid contents, which were significantly higher than those of the normal JIUYAO.					
36750373	5	22	theme	defective	1217:1225	arg1	JIUYAO					1227:1232	defective JIUYAO	1217:1232	defective JIUYAO	1217:1232	Significant differences were observed for the content of 21 volatile compounds between defective and normal JIUYAO, 2-pentylfuran could be used as markers of defective JIUYAO.					
36750373	6	23	theme	normal	1340:1345	arg1	JIUYAO					1347:1352	the normal JIUYAO	1336:1352	the normal JIUYAO	1336:1352	Type I defective JIUYAO contained a higher abundance of aerobic Pediococcus and Marivita compared to the normal JIUYAO.					
36750373	1	24	theme	critical	149:156	arg1	agent					169:173	a critical fermenting agent	147:173	a critical fermenting agent in traditional Huangjiu brewing	147:205	BACKGROUND JIUYAO is a critical fermenting agent in traditional Huangjiu brewing and affects the quality of Huangjiu.					
36750373	1	24	theme	critical	149:156	arg1	JIUYAO					137:142	BACKGROUND JIUYAO	126:142	BACKGROUND JIUYAO	126:142	BACKGROUND JIUYAO is a critical fermenting agent in traditional Huangjiu brewing and affects the quality of Huangjiu.					
36750373	2	25	theme	microbial	496:504	arg1	components					439:448	the main components	430:448	the main components	430:448	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	2	25	theme	microbial	496:504	arg1	structure					516:524	microbial community structure	496:524	microbial community structure	496:524	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	8	26	theme	JIUYAO	1477:1482	arg1	quality					1466:1472	The quality	1462:1472	The quality of JIUYAO	1462:1482	CONCLUSION The quality of JIUYAO was significantly affected by the water content.					
36750373	3	27	theme	neutral	782:788	arg1	contents					721:728	the protein contents	709:728	the protein contents	709:728	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	27	theme	neutral	782:788	arg1	activity					799:806	neutral protease activity	782:806	neutral protease activity	782:806	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	0	28	theme	composition	77:87	arg1	differences					13:23	the differences	9:23	the differences of main components, enzyme activities and microbial composition between defective and normal JIUYAO	9:123	Study on the differences of main components, enzyme activities and microbial composition between defective and normal JIUYAO.					
36750373	9	29	from	JIUYAO	1602:1607	arg1	abundances					1554:1563	the different abundances	1540:1563	the different abundances of aerobic and anaerobic bacteria in JIUYAO	1540:1607	Due to the different abundances of aerobic and anaerobic bacteria in JIUYAO, oxygen may also be an important parameter affecting the quality of JIUYAO.					
36750373	4	30	theme	normal	933:938	arg1	group					940:944	the normal group	929:944	the normal group	929:944	Type II defective JIUYAO had significantly lower indices than the normal group except for starch and free amino acid contents, which were significantly higher than those of the normal JIUYAO.					
36750373	2	31	theme	normal	380:385	arg1	JIUYAO					387:392	normal JIUYAO	380:392	normal JIUYAO	380:392	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	0	32	theme	defective	97:105	arg1	JIUYAO					118:123	defective and normal JIUYAO	97:123	defective and normal JIUYAO	97:123	Study on the differences of main components, enzyme activities and microbial composition between defective and normal JIUYAO.					
36750373	9	33	from	bacteria	1590:1597	arg1	JIUYAO					1602:1607	JIUYAO	1602:1607	JIUYAO	1602:1607	Due to the different abundances of aerobic and anaerobic bacteria in JIUYAO, oxygen may also be an important parameter affecting the quality of JIUYAO.					
36750373	2	34	theme	JIUYAO	369:374	arg1	types					350:354	two common types	339:354	two common types of defective JIUYAO and normal JIUYAO	339:392	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	8	35	theme	water	1518:1522	arg1	content					1524:1530	the water content	1514:1530	the water content	1514:1530	CONCLUSION The quality of JIUYAO was significantly affected by the water content.					
36750373	4	36	theme	free	968:971	arg1	acid					979:982	free amino acid	968:982	free amino acid	968:982	Type II defective JIUYAO had significantly lower indices than the normal group except for starch and free amino acid contents, which were significantly higher than those of the normal JIUYAO.					
36750373	9	37	theme	aerobic	1568:1574	arg1	bacteria					1590:1597	aerobic and anaerobic bacteria	1568:1597	aerobic and anaerobic bacteria in JIUYAO	1568:1607	Due to the different abundances of aerobic and anaerobic bacteria in JIUYAO, oxygen may also be an important parameter affecting the quality of JIUYAO.					
36750373	2	38	theme	community	506:514	arg1	components					439:448	the main components	430:448	the main components	430:448	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	2	38	theme	community	506:514	arg1	structure					516:524	microbial community structure	496:524	microbial community structure	496:524	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	4	39	theme	acid	979:982	arg1	contents					984:991	starch and free amino acid contents	957:991	starch and free amino acid contents	957:991	Type II defective JIUYAO had significantly lower indices than the normal group except for starch and free amino acid contents, which were significantly higher than those of the normal JIUYAO.					
36750373	0	40	theme	components	33:42	arg1	differences					13:23	the differences	9:23	the differences of main components, enzyme activities and microbial composition between defective and normal JIUYAO	9:123	Study on the differences of main components, enzyme activities and microbial composition between defective and normal JIUYAO.					
36750373	5	41	theme	volatile	1119:1126	arg1	compounds					1128:1136	21 volatile compounds	1116:1136	21 volatile compounds	1116:1136	Significant differences were observed for the content of 21 volatile compounds between defective and normal JIUYAO, 2-pentylfuran could be used as markers of defective JIUYAO.					
36750373	9	42	theme	anaerobic	1580:1588	arg1	bacteria					1590:1597	aerobic and anaerobic bacteria	1568:1597	aerobic and anaerobic bacteria in JIUYAO	1568:1607	Due to the different abundances of aerobic and anaerobic bacteria in JIUYAO, oxygen may also be an important parameter affecting the quality of JIUYAO.					
36750373	2	43	theme	JIUYAO	387:392	arg1	types					350:354	two common types	339:354	two common types of defective JIUYAO and normal JIUYAO	339:392	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	3	44	theme	normal	852:857	arg1	JIUYAO					859:864	the normal JIUYAO	848:864	the normal JIUYAO	848:864	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	45	dep	type	623:626	arg1	JIUYAO					640:645	defective JIUYAO	630:645	type I defective JIUYAO	623:645	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	2	46	theme	main	434:437	arg1	components					439:448	the main components	430:448	the main components	430:448	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	2	46	theme	main	434:437	arg1	activity					461:468	enzymatic activity	451:468	enzymatic activity	451:468	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	2	46	theme	main	434:437	arg1	substances					480:489	volatile substances	471:489	volatile substances	471:489	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	2	46	theme	main	434:437	arg1	structure					516:524	microbial community structure	496:524	microbial community structure	496:524	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	2	47	from	emphasis	400:407	arg1	comparison					416:425	the comparison	412:425	the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure	412:524	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	2	48	theme	common	343:348	arg1	types					350:354	two common types	339:354	two common types of defective JIUYAO and normal JIUYAO	339:392	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	7	49	theme	Staphylococcus	1435:1448	arg1	abundance					1402:1410	a higher abundance	1393:1410	a higher abundance of anaerobic Mucor and Staphylococcus	1393:1448	Type II defective JIUYAO consisted of a higher abundance of anaerobic Mucor and Staphylococcus.					
36750373	7	50	theme	Mucor	1425:1429	arg1	abundance					1402:1410	a higher abundance	1393:1410	a higher abundance of anaerobic Mucor and Staphylococcus	1393:1448	Type II defective JIUYAO consisted of a higher abundance of anaerobic Mucor and Staphylococcus.					
36750373	10	51	theme	present	1705:1711	arg1	study					1713:1717	the present study	1701:1717	the present study	1701:1717	We believe that the present study offers a theoretical basis for the quality evaluation and control of JIUYAO.					
36750373	1	52	theme	fermenting	158:167	arg1	agent					169:173	a critical fermenting agent	147:173	a critical fermenting agent in traditional Huangjiu brewing	147:205	BACKGROUND JIUYAO is a critical fermenting agent in traditional Huangjiu brewing and affects the quality of Huangjiu.					
36750373	1	52	theme	fermenting	158:167	arg1	JIUYAO					137:142	BACKGROUND JIUYAO	126:142	BACKGROUND JIUYAO	126:142	BACKGROUND JIUYAO is a critical fermenting agent in traditional Huangjiu brewing and affects the quality of Huangjiu.					
36750373	9	53	theme	important	1632:1640	arg1	oxygen					1610:1615	oxygen	1610:1615	oxygen	1610:1615	Due to the different abundances of aerobic and anaerobic bacteria in JIUYAO, oxygen may also be an important parameter affecting the quality of JIUYAO.					
36750373	9	53	theme	important	1632:1640	arg1	parameter					1642:1650	an important parameter	1629:1650	an important parameter affecting the quality of JIUYAO	1629:1682	Due to the different abundances of aerobic and anaerobic bacteria in JIUYAO, oxygen may also be an important parameter affecting the quality of JIUYAO.					
36750373	3	54	theme	normal	690:695	arg1	JIUYAO					697:702	the normal JIUYAO	686:702	the normal JIUYAO	686:702	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	55	theme	starch	545:550	arg1	contents					552:559	RESULTS Water and starch contents	527:559	contents	552:559	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	10	56	theme	theoretical	1728:1738	arg1	basis					1740:1744	a theoretical basis	1726:1744	a theoretical basis for the quality evaluation and control of JIUYAO	1726:1793	We believe that the present study offers a theoretical basis for the quality evaluation and control of JIUYAO.					
36750373	1	57	theme	Huangjiu	190:197	arg1	brewing					199:205	traditional Huangjiu brewing	178:205	traditional Huangjiu brewing	178:205	BACKGROUND JIUYAO is a critical fermenting agent in traditional Huangjiu brewing and affects the quality of Huangjiu.					
36750373	7	58	theme	defective	1363:1371	arg1	JIUYAO					1373:1378	Type II defective JIUYAO	1355:1378	Type II defective JIUYAO	1355:1378	Type II defective JIUYAO consisted of a higher abundance of anaerobic Mucor and Staphylococcus.					
36750373	3	59	theme	protease	567:574	arg1	activity					576:583	acid protease activity	562:583	acid protease activity	562:583	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	9	60	theme	JIUYAO	1677:1682	arg1	quality					1666:1672	the quality	1662:1672	the quality of JIUYAO	1662:1682	Due to the different abundances of aerobic and anaerobic bacteria in JIUYAO, oxygen may also be an important parameter affecting the quality of JIUYAO.					
36750373	7	61	theme	Type	1355:1358	arg1	JIUYAO					1373:1378	Type II defective JIUYAO	1355:1378	Type II defective JIUYAO	1355:1378	Type II defective JIUYAO consisted of a higher abundance of anaerobic Mucor and Staphylococcus.					
36750373	10	62	theme	quality	1754:1760	arg1	evaluation					1762:1771	the quality evaluation and control	1750:1783	evaluation	1762:1771	We believe that the present study offers a theoretical basis for the quality evaluation and control of JIUYAO.					
36750373	5	63	dep	2-pentylfuran	1175:1187	arg1	used					1198:1201	used	1198:1201	could be used as markers of defective JIUYAO	1189:1232	Significant differences were observed for the content of 21 volatile compounds between defective and normal JIUYAO, 2-pentylfuran could be used as markers of defective JIUYAO.					
36750373	3	64	theme	liquefaction	731:742	arg1	contents					721:728	the protein contents	709:728	the protein contents	709:728	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	64	theme	liquefaction	731:742	arg1	capability					744:753	liquefaction capability	731:753	liquefaction capability	731:753	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	9	65	from	abundances	1554:1563	arg1	JIUYAO					1602:1607	JIUYAO	1602:1607	JIUYAO	1602:1607	Due to the different abundances of aerobic and anaerobic bacteria in JIUYAO, oxygen may also be an important parameter affecting the quality of JIUYAO.					
36750373	0	66	theme	activities	52:61	arg1	differences					13:23	the differences	9:23	the differences of main components, enzyme activities and microbial composition between defective and normal JIUYAO	9:123	Study on the differences of main components, enzyme activities and microbial composition between defective and normal JIUYAO.					
36750373	0	67	theme	microbial	67:75	arg1	composition					77:87	microbial composition	67:87	microbial composition	67:87	Study on the differences of main components, enzyme activities and microbial composition between defective and normal JIUYAO.					
36750373	1	68	from	agent	169:173	arg1	brewing					199:205	traditional Huangjiu brewing	178:205	traditional Huangjiu brewing	178:205	BACKGROUND JIUYAO is a critical fermenting agent in traditional Huangjiu brewing and affects the quality of Huangjiu.					
36750373	2	69	theme	enzymatic	451:459	arg1	components					439:448	the main components	430:448	the main components	430:448	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	2	69	theme	enzymatic	451:459	arg1	activity					461:468	enzymatic activity	451:468	enzymatic activity	451:468	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	5	70	theme	JIUYAO	1227:1232	arg1	markers					1206:1212	markers	1206:1212	markers of defective JIUYAO	1206:1232	Significant differences were observed for the content of 21 volatile compounds between defective and normal JIUYAO, 2-pentylfuran could be used as markers of defective JIUYAO.					
36750373	3	71	theme	protease	790:797	arg1	contents					721:728	the protein contents	709:728	the protein contents	709:728	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	71	theme	protease	790:797	arg1	activity					799:806	neutral protease activity	782:806	neutral protease activity	782:806	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	5	72	theme	Significant	1059:1069	arg1	differences					1071:1081	Significant differences	1059:1081	Significant differences	1059:1081	Significant differences were observed for the content of 21 volatile compounds between defective and normal JIUYAO, 2-pentylfuran could be used as markers of defective JIUYAO.					
36750373	2	73	theme	defective	359:367	arg1	JIUYAO					369:374	defective JIUYAO	359:374	defective JIUYAO	359:374	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	8	74	dep	CONCLUSION	1451:1460	arg1	affected					1502:1509	affected	1502:1509	was significantly affected by the water content	1484:1530	CONCLUSION The quality of JIUYAO was significantly affected by the water content.					
36750373	9	75	theme	different	1544:1552	arg1	abundances					1554:1563	the different abundances	1540:1563	the different abundances of aerobic and anaerobic bacteria in JIUYAO	1540:1607	Due to the different abundances of aerobic and anaerobic bacteria in JIUYAO, oxygen may also be an important parameter affecting the quality of JIUYAO.					
36750373	3	76	theme	type	623:626	arg1	capability					609:618	the esterification capability	590:618	the esterification capability of type I defective JIUYAO	590:645	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	76	theme	type	623:626	arg1	activity					576:583	acid protease activity	562:583	acid protease activity	562:583	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	76	theme	type	623:626	arg1	lower					666:670	lower	666:670	lower	666:670	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	76	theme	type	623:626	arg1	Water					535:539	RESULTS Water and starch contents	527:559	Water	535:539	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	3	76	theme	type	623:626	arg1	contents					552:559	RESULTS Water and starch contents	527:559	contents	552:559	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	0	77	theme	main	28:31	arg1	components					33:42	main components	28:42	main components	28:42	Study on the differences of main components, enzyme activities and microbial composition between defective and normal JIUYAO.					
36750373	4	78	contain	had	892:894	arg2	indices					916:922	significantly lower indices	896:922	significantly lower indices	896:922	Type II defective JIUYAO had significantly lower indices than the normal group except for starch and free amino acid contents, which were significantly higher than those of the normal JIUYAO.					
36750373	4	78	contain	had	892:894	arg1	JIUYAO					885:890	Type II defective JIUYAO	867:890	Type II defective JIUYAO	867:890	Type II defective JIUYAO had significantly lower indices than the normal group except for starch and free amino acid contents, which were significantly higher than those of the normal JIUYAO.					
36750373	2	79	theme	components	439:448	arg1	comparison					416:425	the comparison	412:425	the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure	412:524	To assess and monitor the quality of JIUYAO effectively, we determined the differences between two common types of defective JIUYAO and normal JIUYAO, with emphasis on the comparison of the main components, enzymatic activity, volatile substances, and microbial community structure.					
36750373	6	80	theme	Marivita	1315:1322	arg1	abundance					1278:1286	a higher abundance	1269:1286	a higher abundance of aerobic Pediococcus and Marivita compared to the normal JIUYAO	1269:1352	Type I defective JIUYAO contained a higher abundance of aerobic Pediococcus and Marivita compared to the normal JIUYAO.					
36750373	4	81	theme	amino	973:977	arg1	acid					979:982	free amino acid	968:982	free amino acid	968:982	Type II defective JIUYAO had significantly lower indices than the normal group except for starch and free amino acid contents, which were significantly higher than those of the normal JIUYAO.					
36750373	5	82	theme	compounds	1128:1136	arg1	content					1105:1111	the content	1101:1111	the content of 21 volatile compounds	1101:1136	Significant differences were observed for the content of 21 volatile compounds between defective and normal JIUYAO, 2-pentylfuran could be used as markers of defective JIUYAO.					
36750373	9	83	theme	bacteria	1590:1597	arg1	abundances					1554:1563	the different abundances	1540:1563	the different abundances of aerobic and anaerobic bacteria in JIUYAO	1540:1607	Due to the different abundances of aerobic and anaerobic bacteria in JIUYAO, oxygen may also be an important parameter affecting the quality of JIUYAO.					
36750373	3	84	theme	defective	630:638	arg1	JIUYAO					640:645	defective JIUYAO	630:645	type I defective JIUYAO	623:645	RESULTS Water and starch contents, acid protease activity, and the esterification capability of type I defective JIUYAO were significantly lower than those of the normal JIUYAO, and the protein contents, liquefaction capability, glycation capability, and neutral protease activity were substantially higher than those of the normal JIUYAO.					
36750373	0	85	theme	normal	111:116	arg1	JIUYAO					118:123	defective and normal JIUYAO	97:123	defective and normal JIUYAO	97:123	Study on the differences of main components, enzyme activities and microbial composition between defective and normal JIUYAO.					
36750373	4	86	theme	normal	1044:1049	arg1	JIUYAO					1051:1056	the normal JIUYAO	1040:1056	the normal JIUYAO	1040:1056	Type II defective JIUYAO had significantly lower indices than the normal group except for starch and free amino acid contents, which were significantly higher than those of the normal JIUYAO.					
36750373	1	87	theme	Huangjiu	234:241	arg1	quality					223:229	the quality	219:229	the quality of Huangjiu	219:241	BACKGROUND JIUYAO is a critical fermenting agent in traditional Huangjiu brewing and affects the quality of Huangjiu.					
35974268	0	0	theme	imidacloprid	85:96	arg1	study					118:122	aqueous imidacloprid: sorption mechanism study	77:122	aqueous imidacloprid: sorption mechanism study	77:122	Iron-modified biochar derived from sugarcane bagasse for adequate removal of aqueous imidacloprid: sorption mechanism study.					
35974268	2	1	theme	enormous	405:412	arg1	attention					414:422	enormous attention	405:422	enormous attention	405:422	Low-cost adsorbents, including waste derived materials, clay composites, biochar, and biochar modified materials, have attracted enormous attention for the removal of organic contaminants, including pesticides.					
35974268	3	2	from	400 °C	577:582	arg1	pyrolysis					563:571	pyrolysis	563:571	pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water	563:727	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	0	3	theme	aqueous	77:83	arg1	study					118:122	aqueous imidacloprid: sorption mechanism study	77:122	aqueous imidacloprid: sorption mechanism study	77:122	Iron-modified biochar derived from sugarcane bagasse for adequate removal of aqueous imidacloprid: sorption mechanism study.					
35974268	1	4	theme	promising	161:169	arg1	remediation technology					171:192	a promising remediation technology	159:192	a promising remediation technology to separate organic and inorganic agrochemicals from contaminated soil and water	159:273	Adsorption has been considered as a promising remediation technology to separate organic and inorganic agrochemicals from contaminated soil and water.					
35974268	1	4	theme	promising	161:169	arg1	Adsorption					125:134	Adsorption	125:134	Adsorption	125:134	Adsorption has been considered as a promising remediation technology to separate organic and inorganic agrochemicals from contaminated soil and water.					
35974268	5	5	theme	aqueous	907:913	arg1	solution					915:922	aqueous solution	907:922	aqueous solution	907:922	The adsorbents could remove up to ~ 92% of IMI from aqueous solution at 23.8 mg/L IMI.					
35974268	0	6	theme	sorption	99:106	arg1	study					118:122	aqueous imidacloprid: sorption mechanism study	77:122	aqueous imidacloprid: sorption mechanism study	77:122	Iron-modified biochar derived from sugarcane bagasse for adequate removal of aqueous imidacloprid: sorption mechanism study.					
35974268	4	7	from	model	848:852	arg1	capacity					753:760	The maximum adsorption capacity	730:760	The maximum adsorption capacity of the adsorbent (FeBBC)	730:785	The maximum adsorption capacity of the adsorbent (FeBBC) was calculated as 10.33 (± 1.57) mg/g from Langmuir isotherm model.					
35974268	4	7	from	model	848:852	arg1	mg/g					820:823	10.33 (± 1.57) mg/g	805:823	10.33 (± 1.57) mg/g from Langmuir isotherm model	805:852	The maximum adsorption capacity of the adsorbent (FeBBC) was calculated as 10.33 (± 1.57) mg/g from Langmuir isotherm model.					
35974268	4	8	theme	adsorbent	769:777	arg1	capacity					753:760	The maximum adsorption capacity	730:760	The maximum adsorption capacity of the adsorbent (FeBBC)	730:785	The maximum adsorption capacity of the adsorbent (FeBBC) was calculated as 10.33 (± 1.57) mg/g from Langmuir isotherm model.					
35974268	4	8	theme	adsorbent	769:777	arg1	mg/g					820:823	10.33 (± 1.57) mg/g	805:823	10.33 (± 1.57) mg/g from Langmuir isotherm model	805:852	The maximum adsorption capacity of the adsorbent (FeBBC) was calculated as 10.33 (± 1.57) mg/g from Langmuir isotherm model.					
35974268	5	9	theme	23.8 mg/L	927:935	arg1	IMI					937:939	23.8 mg/L IMI	927:939	23.8 mg/L IMI	927:939	The adsorbents could remove up to ~ 92% of IMI from aqueous solution at 23.8 mg/L IMI.					
35974268	4	10	theme	adsorption	742:751	arg1	capacity					753:760	The maximum adsorption capacity	730:760	The maximum adsorption capacity of the adsorbent (FeBBC)	730:785	The maximum adsorption capacity of the adsorbent (FeBBC) was calculated as 10.33 (± 1.57) mg/g from Langmuir isotherm model.					
35974268	4	10	theme	adsorption	742:751	arg1	mg/g					820:823	10.33 (± 1.57) mg/g	805:823	10.33 (± 1.57) mg/g from Langmuir isotherm model	805:852	The maximum adsorption capacity of the adsorbent (FeBBC) was calculated as 10.33 (± 1.57) mg/g from Langmuir isotherm model.					
35974268	0	11	attach	derived	22:28	arg1	sugarcane					35:43	sugarcane	35:43	sugarcane	35:43	Iron-modified biochar derived from sugarcane bagasse for adequate removal of aqueous imidacloprid: sorption mechanism study.					
35974268	0	11	attach	derived	22:28	arg2	biochar					14:20	Iron-modified biochar	0:20	Iron-modified biochar derived from sugarcane	0:43	Iron-modified biochar derived from sugarcane bagasse for adequate removal of aqueous imidacloprid: sorption mechanism study.					
35974268	7	12	theme	hydrophobic	1225:1235	arg1	interaction					1237:1247	hydrophobic interaction	1225:1247	hydrophobic interaction	1225:1247	Even at highly acidic/basic solution pH, the FeBBC could remove substantial amount of IMI demonstrating hydrophobic interaction and pore diffusion play vital role for removal of IMI.					
35974268	0	13	theme	study	118:122	arg1	removal					66:72	adequate removal	57:72	adequate removal of aqueous imidacloprid: sorption mechanism study	57:122	Iron-modified biochar derived from sugarcane bagasse for adequate removal of aqueous imidacloprid: sorption mechanism study.					
35974268	7	14	theme	IMI	1299:1301	arg1	removal					1288:1294	removal	1288:1294	removal of IMI	1288:1301	Even at highly acidic/basic solution pH, the FeBBC could remove substantial amount of IMI demonstrating hydrophobic interaction and pore diffusion play vital role for removal of IMI.					
35974268	2	15	theme	waste	307:311	arg1	derived materials					313:329	waste derived materials	307:329	waste derived materials	307:329	Low-cost adsorbents, including waste derived materials, clay composites, biochar, and biochar modified materials, have attracted enormous attention for the removal of organic contaminants, including pesticides.					
35974268	2	15	theme	waste	307:311	arg1	composites					337:346	clay composites	332:346	clay composites	332:346	Low-cost adsorbents, including waste derived materials, clay composites, biochar, and biochar modified materials, have attracted enormous attention for the removal of organic contaminants, including pesticides.					
35974268	2	15	theme	waste	307:311	arg1	and biochar modified materials					358:387	and biochar modified materials	358:387	and biochar modified materials	358:387	Low-cost adsorbents, including waste derived materials, clay composites, biochar, and biochar modified materials, have attracted enormous attention for the removal of organic contaminants, including pesticides.					
35974268	2	15	theme	waste	307:311	arg1	biochar					349:355	biochar	349:355	biochar	349:355	Low-cost adsorbents, including waste derived materials, clay composites, biochar, and biochar modified materials, have attracted enormous attention for the removal of organic contaminants, including pesticides.					
35974268	2	16	theme	organic	443:449	arg1	contaminants					451:462	organic contaminants	443:462	organic contaminants	443:462	Low-cost adsorbents, including waste derived materials, clay composites, biochar, and biochar modified materials, have attracted enormous attention for the removal of organic contaminants, including pesticides.					
35974268	2	16	theme	organic	443:449	arg1	pesticides					475:484	pesticides	475:484	pesticides	475:484	Low-cost adsorbents, including waste derived materials, clay composites, biochar, and biochar modified materials, have attracted enormous attention for the removal of organic contaminants, including pesticides.					
35974268	0	17	theme	mechanism	108:116	arg1	study					118:122	aqueous imidacloprid: sorption mechanism study	77:122	aqueous imidacloprid: sorption mechanism study	77:122	Iron-modified biochar derived from sugarcane bagasse for adequate removal of aqueous imidacloprid: sorption mechanism study.					
35974268	8	18	theme	sorption	1332:1339	arg1	slight					1308:1313	slight	1308:1313	slight	1308:1313	The slight improving of IMI sorption with increasing solution pH indicated the sorption was also facilitated through ionic interaction alongside physical sorption.					
35974268	3	19	from	water	723:727	arg1	removal					654:660	the removal	650:660	the removal of a widely used insecticide, namely imidacloprid (IMI) from water	650:727	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	4	20	theme	isotherm	839:846	arg1	model					848:852	Langmuir isotherm model	830:852	Langmuir isotherm model	830:852	The maximum adsorption capacity of the adsorbent (FeBBC) was calculated as 10.33 (± 1.57) mg/g from Langmuir isotherm model.					
35974268	3	21	theme	iron-doped	596:605	arg1	bagasse					638:644	iron-doped base (KOH) activated sugarcane bagasse	596:644	iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water	596:727	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	1	22	theme	separate	197:204	arg1	agrochemicals					228:240	separate organic and inorganic agrochemicals	197:240	separate organic and inorganic agrochemicals from contaminated soil and water	197:273	Adsorption has been considered as a promising remediation technology to separate organic and inorganic agrochemicals from contaminated soil and water.					
35974268	0	23	theme	Iron-modified	0:12	arg1	biochar					14:20	Iron-modified biochar	0:20	Iron-modified biochar derived from sugarcane	0:43	Iron-modified biochar derived from sugarcane bagasse for adequate removal of aqueous imidacloprid: sorption mechanism study.					
35974268	4	24	theme	Langmuir	830:837	arg1	model					848:852	Langmuir isotherm model	830:852	Langmuir isotherm model	830:852	The maximum adsorption capacity of the adsorbent (FeBBC) was calculated as 10.33 (± 1.57) mg/g from Langmuir isotherm model.					
35974268	3	25	theme	base	607:610	arg1	bagasse					638:644	iron-doped base (KOH) activated sugarcane bagasse	596:644	iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water	596:727	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	1	26	theme	organic	206:212	arg1	agrochemicals					228:240	separate organic and inorganic agrochemicals	197:240	separate organic and inorganic agrochemicals from contaminated soil and water	197:273	Adsorption has been considered as a promising remediation technology to separate organic and inorganic agrochemicals from contaminated soil and water.					
35974268	7	27	theme	solution	1149:1156	arg1	pH					1158:1159	highly acidic/basic solution pH	1129:1159	highly acidic/basic solution pH	1129:1159	Even at highly acidic/basic solution pH, the FeBBC could remove substantial amount of IMI demonstrating hydrophobic interaction and pore diffusion play vital role for removal of IMI.					
35974268	3	28	theme	sugarcane	628:636	arg1	bagasse					638:644	iron-doped base (KOH) activated sugarcane bagasse	596:644	iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water	596:727	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	9	29	theme	IMI	1596:1598	arg1	sorption					1584:1591	the sorption	1580:1591	the sorption of IMI	1580:1598	However, physical sorption including hydrophobic interaction and pore-filling interaction plays a vital role in the sorption of IMI.					
35974268	3	30	theme	base-activated	516:529	arg1	FeBBC					540:544	FeBBC	540:544	FeBBC	540:544	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	3	30	theme	base-activated	516:529	arg1	biochar					531:537	iron-modified base-activated biochar	502:537	iron-modified base-activated biochar (FeBBC)	502:545	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	4	31	theme	maximum	734:740	arg1	capacity					753:760	The maximum adsorption capacity	730:760	The maximum adsorption capacity of the adsorbent (FeBBC)	730:785	The maximum adsorption capacity of the adsorbent (FeBBC) was calculated as 10.33 (± 1.57) mg/g from Langmuir isotherm model.					
35974268	4	31	theme	maximum	734:740	arg1	mg/g					820:823	10.33 (± 1.57) mg/g	805:823	10.33 (± 1.57) mg/g from Langmuir isotherm model	805:852	The maximum adsorption capacity of the adsorbent (FeBBC) was calculated as 10.33 (± 1.57) mg/g from Langmuir isotherm model.					
35974268	8	32	theme	physical	1449:1456	arg1	sorption					1458:1465	physical sorption	1449:1465	physical sorption	1449:1465	The slight improving of IMI sorption with increasing solution pH indicated the sorption was also facilitated through ionic interaction alongside physical sorption.					
35974268	2	33	theme	clay	332:335	arg1	derived materials					313:329	waste derived materials	307:329	waste derived materials	307:329	Low-cost adsorbents, including waste derived materials, clay composites, biochar, and biochar modified materials, have attracted enormous attention for the removal of organic contaminants, including pesticides.					
35974268	2	33	theme	clay	332:335	arg1	composites					337:346	clay composites	332:346	clay composites	332:346	Low-cost adsorbents, including waste derived materials, clay composites, biochar, and biochar modified materials, have attracted enormous attention for the removal of organic contaminants, including pesticides.					
35974268	1	34	theme	inorganic	218:226	arg1	agrochemicals					228:240	separate organic and inorganic agrochemicals	197:240	separate organic and inorganic agrochemicals from contaminated soil and water	197:273	Adsorption has been considered as a promising remediation technology to separate organic and inorganic agrochemicals from contaminated soil and water.					
35974268	9	35	theme	 physical	1476:1484	arg1	sorption					1486:1493	 physical sorption	1476:1493	 physical sorption including hydrophobic interaction and pore-filling interaction	1476:1556	However, physical sorption including hydrophobic interaction and pore-filling interaction plays a vital role in the sorption of IMI.					
35974268	7	36	theme	acidic/basic	1136:1147	arg1	pH					1158:1159	highly acidic/basic solution pH	1129:1159	highly acidic/basic solution pH	1129:1159	Even at highly acidic/basic solution pH, the FeBBC could remove substantial amount of IMI demonstrating hydrophobic interaction and pore diffusion play vital role for removal of IMI.					
35974268	3	37	theme	activated	618:626	arg1	bagasse					638:644	iron-doped base (KOH) activated sugarcane bagasse	596:644	iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water	596:727	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	2	38	theme	contaminants	451:462	arg1	removal					432:438	the removal	428:438	the removal of organic contaminants, including pesticides	428:484	Low-cost adsorbents, including waste derived materials, clay composites, biochar, and biochar modified materials, have attracted enormous attention for the removal of organic contaminants, including pesticides.					
35974268	0	39	theme	adequate	57:64	arg1	removal					66:72	adequate removal	57:72	adequate removal of aqueous imidacloprid: sorption mechanism study	57:122	Iron-modified biochar derived from sugarcane bagasse for adequate removal of aqueous imidacloprid: sorption mechanism study.					
35974268	8	40	theme	solution	1357:1364	arg1	pH					1366:1367	solution pH	1357:1367	solution pH	1357:1367	The slight improving of IMI sorption with increasing solution pH indicated the sorption was also facilitated through ionic interaction alongside physical sorption.					
35974268	6	41	theme	Experimental	942:953	arg1	data					955:958	Experimental data	942:958	Experimental data	942:958	Experimental data fitted well with the Freundlich model and pseudo-second-order model, demonstrating physisorption, as well as chemosorption, contributed to the sorption process.					
35974268	3	42	theme	iron-modified	502:514	arg1	FeBBC					540:544	FeBBC	540:544	FeBBC	540:544	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	3	42	theme	iron-modified	502:514	arg1	biochar					531:537	iron-modified base-activated biochar	502:537	iron-modified base-activated biochar (FeBBC)	502:545	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	7	43	theme	vital	1273:1277	arg1	role					1279:1282	vital role	1273:1282	vital role	1273:1282	Even at highly acidic/basic solution pH, the FeBBC could remove substantial amount of IMI demonstrating hydrophobic interaction and pore diffusion play vital role for removal of IMI.					
35974268	1	44	from	water	269:273	arg1	agrochemicals					228:240	separate organic and inorganic agrochemicals	197:240	separate organic and inorganic agrochemicals from contaminated soil and water	197:273	Adsorption has been considered as a promising remediation technology to separate organic and inorganic agrochemicals from contaminated soil and water.					
35974268	9	45	theme	vital	1566:1570	arg1	role					1572:1575	a vital role	1564:1575	a vital role	1564:1575	However, physical sorption including hydrophobic interaction and pore-filling interaction plays a vital role in the sorption of IMI.					
35974268	3	46	theme	KOH	613:615	arg1	bagasse					638:644	iron-doped base (KOH) activated sugarcane bagasse	596:644	iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water	596:727	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	7	47	theme	substantial	1185:1195	arg1	IMI					1207:1209	IMI	1207:1209	IMI	1207:1209	Even at highly acidic/basic solution pH, the FeBBC could remove substantial amount of IMI demonstrating hydrophobic interaction and pore diffusion play vital role for removal of IMI.					
35974268	7	47	theme	substantial	1185:1195	arg1	amount					1197:1202	substantial amount	1185:1202	substantial amount of IMI	1185:1209	Even at highly acidic/basic solution pH, the FeBBC could remove substantial amount of IMI demonstrating hydrophobic interaction and pore diffusion play vital role for removal of IMI.					
35974268	9	48	theme	hydrophobic	1505:1515	arg1	interaction					1517:1527	hydrophobic interaction	1505:1527	hydrophobic interaction	1505:1527	However, physical sorption including hydrophobic interaction and pore-filling interaction plays a vital role in the sorption of IMI.					
35974268	8	49	theme	ionic	1421:1425	arg1	interaction					1427:1437	ionic interaction	1421:1437	ionic interaction alongside physical sorption	1421:1465	The slight improving of IMI sorption with increasing solution pH indicated the sorption was also facilitated through ionic interaction alongside physical sorption.					
35974268	5	50	theme	IMI	898:900	arg1	IMI					898:900	IMI	898:900	IMI	898:900	The adsorbents could remove up to ~ 92% of IMI from aqueous solution at 23.8 mg/L IMI.					
35974268	5	50	theme	IMI	898:900	arg1	%					893:893	up to ~ 92%	883:893	up to ~ 92% of IMI	883:900	The adsorbents could remove up to ~ 92% of IMI from aqueous solution at 23.8 mg/L IMI.					
35974268	1	51	theme	contaminated	247:258	arg1	soil					260:263	contaminated soil	247:263	contaminated soil	247:263	Adsorption has been considered as a promising remediation technology to separate organic and inorganic agrochemicals from contaminated soil and water.					
35974268	1	52	from	soil	260:263	arg1	agrochemicals					228:240	separate organic and inorganic agrochemicals	197:240	separate organic and inorganic agrochemicals from contaminated soil and water	197:273	Adsorption has been considered as a promising remediation technology to separate organic and inorganic agrochemicals from contaminated soil and water.					
35974268	3	53	theme	used	674:677	arg1	insecticide					679:689	a widely used insecticide	665:689	a widely used insecticide	665:689	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	3	53	theme	used	674:677	arg1	imidacloprid					699:710	imidacloprid	699:710	imidacloprid (IMI)	699:716	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	8	54	theme	IMI	1328:1330	arg1	sorption					1332:1339	IMI sorption	1328:1339	IMI sorption with increasing solution pH	1328:1367	The slight improving of IMI sorption with increasing solution pH indicated the sorption was also facilitated through ionic interaction alongside physical sorption.					
35974268	7	55	theme	pore	1253:1256	arg1	diffusion					1258:1266	pore diffusion	1253:1266	pore diffusion	1253:1266	Even at highly acidic/basic solution pH, the FeBBC could remove substantial amount of IMI demonstrating hydrophobic interaction and pore diffusion play vital role for removal of IMI.					
35974268	6	56	theme	sorption	1103:1110	arg1	process					1112:1118	the sorption process	1099:1118	the sorption process	1099:1118	Experimental data fitted well with the Freundlich model and pseudo-second-order model, demonstrating physisorption, as well as chemosorption, contributed to the sorption process.					
35974268	7	57	theme	IMI	1207:1209	arg1	IMI					1207:1209	IMI	1207:1209	IMI	1207:1209	Even at highly acidic/basic solution pH, the FeBBC could remove substantial amount of IMI demonstrating hydrophobic interaction and pore diffusion play vital role for removal of IMI.					
35974268	7	57	theme	IMI	1207:1209	arg1	amount					1197:1202	substantial amount	1185:1202	substantial amount of IMI	1185:1209	Even at highly acidic/basic solution pH, the FeBBC could remove substantial amount of IMI demonstrating hydrophobic interaction and pore diffusion play vital role for removal of IMI.					
35974268	3	58	theme	insecticide	679:689	arg1	removal					654:660	the removal	650:660	the removal of a widely used insecticide, namely imidacloprid (IMI) from water	650:727	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	2	59	theme	Low-cost	276:283	arg1	derived materials					313:329	waste derived materials	307:329	waste derived materials	307:329	Low-cost adsorbents, including waste derived materials, clay composites, biochar, and biochar modified materials, have attracted enormous attention for the removal of organic contaminants, including pesticides.					
35974268	2	59	theme	Low-cost	276:283	arg1	adsorbents					285:294	Low-cost adsorbents	276:294	Low-cost adsorbents	276:294	Low-cost adsorbents, including waste derived materials, clay composites, biochar, and biochar modified materials, have attracted enormous attention for the removal of organic contaminants, including pesticides.					
35974268	9	60	theme	pore-filling	1533:1544	arg1	interaction					1546:1556	pore-filling interaction	1533:1556	pore-filling interaction	1533:1556	However, physical sorption including hydrophobic interaction and pore-filling interaction plays a vital role in the sorption of IMI.					
35974268	3	61	theme	bagasse	638:644	arg1	pyrolysis					563:571	pyrolysis	563:571	pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water	563:727	In this study, iron-modified base-activated biochar (FeBBC) was prepared by pyrolysis (at 400 °C for 1 h) of iron-doped base (KOH) activated sugarcane bagasse for the removal of a widely used insecticide, namely imidacloprid (IMI) from water.					
35974268	6	62	theme	pseudo-second-order	1002:1020	arg1	model					1022:1026	pseudo-second-order model	1002:1026	pseudo-second-order model	1002:1026	Experimental data fitted well with the Freundlich model and pseudo-second-order model, demonstrating physisorption, as well as chemosorption, contributed to the sorption process.					
35974268	6	63	theme	Freundlich	981:990	arg1	model					992:996	the Freundlich model	977:996	the Freundlich model	977:996	Experimental data fitted well with the Freundlich model and pseudo-second-order model, demonstrating physisorption, as well as chemosorption, contributed to the sorption process.					
36364023	1	0	from	acid	211:214	arg1	presence					286:293	the presence	282:293	the presence of specific carbon and nitrogen sources	282:333	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	13	1	theme	acid	2316:2319	arg1	fumaric					2297:2303	fumaric	2297:2303	fumaric	2297:2303	Large amounts of both fumaric and lactic acid were produced, particularly under high-nitrogen treatments.					
36364023	13	1	theme	acid	2316:2319	arg1	acid					2316:2319	lactic acid	2309:2319	lactic acid	2309:2319	Large amounts of both fumaric and lactic acid were produced, particularly under high-nitrogen treatments.					
36364023	13	1	theme	acid	2316:2319	arg1	amounts					2281:2287	Large amounts	2275:2287	Large amounts of both fumaric and lactic acid	2275:2319	Large amounts of both fumaric and lactic acid were produced, particularly under high-nitrogen treatments.					
36364023	3	2	theme	almonds	436:442	arg1	symptoms					445:452	symptoms	445:452	symptoms	445:452	Rhizopus stolonifer causes hull rot disease in almonds, symptoms of which have been previously associated with the fungus's production of fumaric acid.					
36364023	5	3	dep	in	1115:1116	arg1	vitro					1118:1122	vitro	1118:1122	vitro	1118:1122	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	1	4	theme	citric	230:235	arg1	acid					237:240	citric acid	230:240	citric acid	230:240	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	4	5	theme	nitrogen	724:731	arg1	sources					733:739	two nitrogen sources	720:739	two nitrogen sources (asparagine and ammonium sulphate)	720:774	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	5	theme	nitrogen	724:731	arg1	asparagine					742:751	asparagine	742:751	asparagine	742:751	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	5	theme	nitrogen	724:731	arg1	sulphate					766:773	ammonium sulphate	757:773	ammonium sulphate	757:773	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	9	6	theme	stolonifer	1680:1689	arg1	This					1630:1633	This	1630:1633	This	1630:1633	This is the first report, to our knowledge, of R. stolonifer producing lactic acid in preference to fumaric acid.					
36364023	9	6	theme	stolonifer	1680:1689	arg1	report					1648:1653	the first report	1638:1653	the first report	1638:1653	This is the first report, to our knowledge, of R. stolonifer producing lactic acid in preference to fumaric acid.					
36364023	9	7	theme	lactic	1701:1706	arg1	acid					1708:1711	lactic acid	1701:1711	lactic acid in preference to fumaric acid	1701:1741	This is the first report, to our knowledge, of R. stolonifer producing lactic acid in preference to fumaric acid.					
36364023	4	8	theme	almond	591:596	arg1	hulls					598:602	infected almond hulls	582:602	infected almond hulls	582:602	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	8	9	theme	carbon	1536:1541	arg1	sucrose					1521:1527	sucrose	1521:1527	sucrose	1521:1527	Isolate 19A-0069, however, preferred sucrose as the carbon source, and Isolate 19A-0030 produced higher amounts of lactic acid than fumaric acid.					
36364023	8	9	theme	carbon	1536:1541	arg1	source					1543:1548	the carbon source	1532:1548	the carbon source	1532:1548	Isolate 19A-0069, however, preferred sucrose as the carbon source, and Isolate 19A-0030 produced higher amounts of lactic acid than fumaric acid.					
36364023	4	10	theme	hull	799:802	arg1	composition					804:814	almond hull composition	792:814	almond hull composition	792:814	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	10	11	theme	infected	1922:1929	arg1	fruit					1946:1950	infected and uninfected fruit	1922:1950	infected and uninfected fruit	1922:1950	Additionally, R. stolonifer isolate 19-0030 was inoculated into Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments, and hull compositions of infected and uninfected fruit were analysed using 1H NMR-based metabolomics.					
36364023	6	12	theme	acid	1161:1164	arg1	production					1166:1175	acid production	1161:1175	acid production	1161:1175	Sugar metabolisation and acid production were significantly influenced by sugar source and isolates, with five isolates depleting glucose most rapidly, followed by fructose, sucrose, and then xylose.					
36364023	1	13	theme	carbon	307:312	arg1	presence					286:293	the presence	282:293	the presence of specific carbon and nitrogen sources	282:333	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	1	14	theme	primary	175:181	arg1	acid					211:214	fumaric acid	203:214	fumaric acid	203:214	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	1	14	theme	primary	175:181	arg1	metabolites					183:193	primary metabolites	175:193	primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources	175:333	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	14	15	theme	dilute	2415:2420	arg1	solutions					2422:2430	dilute solutions	2415:2430	dilute solutions of fumaric acid or lactic acid	2415:2461	Moreover, almond shoots placed in dilute solutions of fumaric acid or lactic acid developed leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field, suggesting both acids are involved in causing disease.					
36364023	4	16	theme	stolonifer	560:569	arg1	isolates					545:552	Six isolates	541:552	Six isolates of R. stolonifer taken from infected almond hulls	541:602	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	1	17	theme	nitrogen	318:325	arg1	sources					327:333	specific carbon and nitrogen sources	298:333	sources	327:333	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	9	18	from	acid	1708:1711	arg1	preference					1716:1725	preference	1716:1725	preference	1716:1725	This is the first report, to our knowledge, of R. stolonifer producing lactic acid in preference to fumaric acid.					
36364023	10	19	theme	R.	1758:1759	arg1	isolate					1772:1778	R. stolonifer isolate 19-0030	1758:1786	R. stolonifer isolate 19-0030	1758:1786	Additionally, R. stolonifer isolate 19-0030 was inoculated into Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments, and hull compositions of infected and uninfected fruit were analysed using 1H NMR-based metabolomics.					
36364023	5	20	theme	R.	916:917	arg1	stolonifer					919:928	R. stolonifer	916:928	R. stolonifer	916:928	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	14	21	theme	almond	2391:2396	arg1	shoots					2398:2403	almond shoots	2391:2403	almond shoots placed in dilute solutions of fumaric acid or lactic acid	2391:2461	Moreover, almond shoots placed in dilute solutions of fumaric acid or lactic acid developed leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field, suggesting both acids are involved in causing disease.					
36364023	10	22	theme	uninfected	1935:1944	arg1	fruit					1946:1950	infected and uninfected fruit	1922:1950	infected and uninfected fruit	1922:1950	Additionally, R. stolonifer isolate 19-0030 was inoculated into Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments, and hull compositions of infected and uninfected fruit were analysed using 1H NMR-based metabolomics.					
36364023	11	23	dep	high-nitrogen	2145:2157	arg1	treatments					2169:2178	treatments	2169:2178	treatments	2169:2178	Glucose and asparagine content of uninfected hulls was influenced by the nitrogen and water treatments provided to the trees, being higher in the high-nitrogen and water treatments.					
36364023	4	24	used	used	820:823	arg2	isolates					545:552	Six isolates	541:552	Six isolates of R. stolonifer taken from infected almond hulls	541:602	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	1	25	theme	fungal	114:119	arg1	Rhizopus					127:134	the fungal genus Rhizopus	110:134	the fungal genus Rhizopus	110:134	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	5	26	dep	metabolites	1050:1060	arg1	i.e.					1063:1066	three metabolites, i.e., fumaric acid, lactic acid, and ethanol	1044:1106	i.e.	1063:1066	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	14	27	theme	hull	2533:2536	arg1	disease					2542:2548	hull rot disease	2533:2548	hull rot disease in the field	2533:2561	Moreover, almond shoots placed in dilute solutions of fumaric acid or lactic acid developed leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field, suggesting both acids are involved in causing disease.					
36364023	10	28	theme	NMR-based	1975:1983	arg1	metabolomics					1985:1996	1H NMR-based metabolomics	1972:1996	1H NMR-based metabolomics	1972:1996	Additionally, R. stolonifer isolate 19-0030 was inoculated into Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments, and hull compositions of infected and uninfected fruit were analysed using 1H NMR-based metabolomics.					
36364023	5	29	theme	in	1115:1116	arg1	conditions					1124:1133	in vitro conditions	1115:1133	in vitro conditions	1115:1133	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	0	30	theme	Causal	58:63	arg1	Production					0:9	Production	0:9	Production of Primary Metabolites by Rhizopus stolonifer	0:55	Production of Primary Metabolites by Rhizopus stolonifer, Causal Agent of Almond Hull Rot Disease.					
36364023	0	30	theme	Causal	58:63	arg1	Agent					65:69	Causal Agent	58:69	Causal Agent of Almond Hull Rot Disease	58:96	Production of Primary Metabolites by Rhizopus stolonifer, Causal Agent of Almond Hull Rot Disease.					
36364023	3	31	theme	acid	535:538	arg1	production					513:522	the fungus's production	500:522	the fungus's production of fumaric acid	500:538	Rhizopus stolonifer causes hull rot disease in almonds, symptoms of which have been previously associated with the fungus's production of fumaric acid.					
36364023	8	32	theme	lactic	1599:1604	arg1	acid					1606:1609	lactic acid	1599:1609	lactic acid	1599:1609	Isolate 19A-0069, however, preferred sucrose as the carbon source, and Isolate 19A-0030 produced higher amounts of lactic acid than fumaric acid.					
36364023	6	33	theme	sugar	1210:1214	arg1	source					1216:1221	sugar source	1210:1221	sugar source	1210:1221	Sugar metabolisation and acid production were significantly influenced by sugar source and isolates, with five isolates depleting glucose most rapidly, followed by fructose, sucrose, and then xylose.					
36364023	5	34	theme	extent	991:996	arg1	xylose					998:1003	a lesser extent xylose	982:1003	a lesser extent xylose	982:1003	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	4	35	dep	sources	667:673	arg1	sucrose					695:701	sucrose	695:701	sucrose	695:701	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	35	dep	sources	667:673	arg1	xylose					708:713	xylose	708:713	xylose	708:713	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	35	dep	sources	667:673	arg1	fructose					685:692	fructose	685:692	fructose	685:692	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	35	dep	sources	667:673	arg1	glucose					676:682	glucose	676:682	glucose	676:682	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	35	dep	sources	667:673	arg1	sources					667:673	four carbon sources	655:673	four carbon sources (glucose, fructose, sucrose, and xylose)	655:714	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	11	36	theme	uninfected	2033:2042	arg1	hulls					2044:2048	uninfected hulls	2033:2048	uninfected hulls	2033:2048	Glucose and asparagine content of uninfected hulls was influenced by the nitrogen and water treatments provided to the trees, being higher in the high-nitrogen and water treatments.					
36364023	10	37	theme	almond	1818:1823	arg1	fruit					1825:1829	Nonpareil almond fruit	1808:1829	Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments	1808:1894	Additionally, R. stolonifer isolate 19-0030 was inoculated into Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments, and hull compositions of infected and uninfected fruit were analysed using 1H NMR-based metabolomics.					
36364023	0	38	theme	Hull	81:84	arg1	Disease					90:96	Almond Hull Rot Disease	74:96	Almond Hull Rot Disease	74:96	Production of Primary Metabolites by Rhizopus stolonifer, Causal Agent of Almond Hull Rot Disease.					
36364023	5	39	theme	nuclear	845:851	arg1	NMR					876:878	1H NMR	873:878	1H NMR	873:878	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	5	39	theme	nuclear	845:851	arg1	resonance					862:870	Proton nuclear magnetic resonance	838:870	Proton nuclear magnetic resonance (1H NMR)-based metabolomics	838:898	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	0	40	theme	Disease	90:96	arg1	Production					0:9	Production	0:9	Production of Primary Metabolites by Rhizopus stolonifer	0:55	Production of Primary Metabolites by Rhizopus stolonifer, Causal Agent of Almond Hull Rot Disease.					
36364023	0	40	theme	Disease	90:96	arg1	Agent					65:69	Causal Agent	58:69	Causal Agent of Almond Hull Rot Disease	58:96	Production of Primary Metabolites by Rhizopus stolonifer, Causal Agent of Almond Hull Rot Disease.					
36364023	5	41	theme	resonance	862:870	arg1	metabolomics					887:898	Proton nuclear magnetic resonance (1H NMR)-based metabolomics	838:898	Proton nuclear magnetic resonance (1H NMR)-based metabolomics	838:898	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	2	42	theme	plant	368:372	arg1	pathogenicity					374:386	plant pathogenicity	368:386	plant pathogenicity	368:386	This ability has been linked to plant pathogenicity.					
36364023	4	43	dep	sources	733:739	arg1	sources					733:739	two nitrogen sources	720:739	two nitrogen sources (asparagine and ammonium sulphate)	720:774	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	43	dep	sources	733:739	arg1	asparagine					742:751	asparagine	742:751	asparagine	742:751	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	43	dep	sources	733:739	arg1	sulphate					766:773	ammonium sulphate	757:773	ammonium sulphate	757:773	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	1	44	from	Species	99:105	arg1	Rhizopus					127:134	the fungal genus Rhizopus	110:134	the fungal genus Rhizopus	110:134	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	14	45	theme	strike	2508:2513	arg1	symptoms					2516:2523	the 'strike' symptoms	2503:2523	the 'strike' symptoms seen in hull rot disease in the field	2503:2561	Moreover, almond shoots placed in dilute solutions of fumaric acid or lactic acid developed leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field, suggesting both acids are involved in causing disease.					
36364023	4	46	theme	carbon	660:665	arg1	sucrose					695:701	sucrose	695:701	sucrose	695:701	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	46	theme	carbon	660:665	arg1	xylose					708:713	xylose	708:713	xylose	708:713	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	46	theme	carbon	660:665	arg1	fructose					685:692	fructose	685:692	fructose	685:692	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	46	theme	carbon	660:665	arg1	glucose					676:682	glucose	676:682	glucose	676:682	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	46	theme	carbon	660:665	arg1	sources					667:673	four carbon sources	655:673	four carbon sources (glucose, fructose, sucrose, and xylose)	655:714	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	3	47	theme	Rhizopus	389:396	arg1	stolonifer					398:407	Rhizopus stolonifer	389:407	Rhizopus stolonifer	389:407	Rhizopus stolonifer causes hull rot disease in almonds, symptoms of which have been previously associated with the fungus's production of fumaric acid.					
36364023	0	48	theme	Primary	14:20	arg1	Metabolites					22:32	Primary Metabolites	14:32	Primary Metabolites	14:32	Production of Primary Metabolites by Rhizopus stolonifer, Causal Agent of Almond Hull Rot Disease.					
36364023	7	49	theme	maximum	1340:1346	arg1	amounts					1348:1354	The maximum amounts	1336:1354	The maximum amounts of metabolites	1336:1369	The maximum amounts of metabolites were produced when glucose was the carbon source, with fumaric acid produced in higher amounts than lactic acid.					
36364023	7	49	theme	maximum	1340:1346	arg1	metabolites					1359:1369	metabolites	1359:1369	metabolites	1359:1369	The maximum amounts of metabolites were produced when glucose was the carbon source, with fumaric acid produced in higher amounts than lactic acid.					
36364023	14	50	theme	similar	2492:2498	arg1	symptoms					2478:2485	leaf symptoms	2473:2485	leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field	2473:2561	Moreover, almond shoots placed in dilute solutions of fumaric acid or lactic acid developed leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field, suggesting both acids are involved in causing disease.					
36364023	3	51	theme	rot	421:423	arg1	disease					425:431	hull rot disease	416:431	hull rot disease in almonds, symptoms of which have been previously associated with the fungus's production of fumaric acid	416:538	Rhizopus stolonifer causes hull rot disease in almonds, symptoms of which have been previously associated with the fungus's production of fumaric acid.					
36364023	13	52	theme	high-nitrogen	2355:2367	arg1	treatments					2369:2378	high-nitrogen treatments	2355:2378	high-nitrogen treatments	2355:2378	Large amounts of both fumaric and lactic acid were produced, particularly under high-nitrogen treatments.					
36364023	1	53	theme	lesser	253:258	arg1	extent					260:265	a lesser extent	251:265	a lesser extent	251:265	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	9	54	theme	first	1642:1646	arg1	This					1630:1633	This	1630:1633	This	1630:1633	This is the first report, to our knowledge, of R. stolonifer producing lactic acid in preference to fumaric acid.					
36364023	9	54	theme	first	1642:1646	arg1	report					1648:1653	the first report	1638:1653	the first report	1638:1653	This is the first report, to our knowledge, of R. stolonifer producing lactic acid in preference to fumaric acid.					
36364023	10	55	from	fruit	1825:1829	arg1	trees					1834:1838	trees	1834:1838	trees grown under high- and low-nitrogen and water treatments	1834:1894	Additionally, R. stolonifer isolate 19-0030 was inoculated into Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments, and hull compositions of infected and uninfected fruit were analysed using 1H NMR-based metabolomics.					
36364023	5	56	theme	1H	873:874	arg1	NMR					876:878	1H NMR	873:878	1H NMR	873:878	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	5	56	theme	1H	873:874	arg1	resonance					862:870	Proton nuclear magnetic resonance	838:870	Proton nuclear magnetic resonance (1H NMR)-based metabolomics	838:898	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	14	57	theme	lactic	2451:2456	arg1	acid					2458:2461	lactic acid	2451:2461	lactic acid	2451:2461	Moreover, almond shoots placed in dilute solutions of fumaric acid or lactic acid developed leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field, suggesting both acids are involved in causing disease.					
36364023	13	58	theme	lactic	2309:2314	arg1	acid					2316:2319	lactic acid	2309:2319	lactic acid	2309:2319	Large amounts of both fumaric and lactic acid were produced, particularly under high-nitrogen treatments.					
36364023	1	59	theme	lactic	217:222	arg1	acid					224:227	lactic acid	217:227	lactic acid	217:227	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	5	60	theme	lesser	984:989	arg1	extent					991:996	a lesser extent	982:996	a lesser extent xylose	982:1003	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	14	61	theme	acid	2443:2446	arg1	solutions					2422:2430	dilute solutions	2415:2430	dilute solutions of fumaric acid or lactic acid	2415:2461	Moreover, almond shoots placed in dilute solutions of fumaric acid or lactic acid developed leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field, suggesting both acids are involved in causing disease.					
36364023	4	62	theme	ammonium	757:764	arg1	sources					733:739	two nitrogen sources	720:739	two nitrogen sources (asparagine and ammonium sulphate)	720:774	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	62	theme	ammonium	757:764	arg1	sulphate					766:773	ammonium sulphate	757:773	ammonium sulphate	757:773	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	10	63	theme	hull	1901:1904	arg1	compositions					1906:1917	hull compositions	1901:1917	hull compositions of infected and uninfected fruit	1901:1950	Additionally, R. stolonifer isolate 19-0030 was inoculated into Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments, and hull compositions of infected and uninfected fruit were analysed using 1H NMR-based metabolomics.					
36364023	11	64	dep	trees	2118:2122	arg1	higher					2131:2136	higher	2131:2136	higher	2131:2136	Glucose and asparagine content of uninfected hulls was influenced by the nitrogen and water treatments provided to the trees, being higher in the high-nitrogen and water treatments.					
36364023	13	65	theme	fumaric	2297:2303	arg1	fumaric					2297:2303	fumaric	2297:2303	fumaric	2297:2303	Large amounts of both fumaric and lactic acid were produced, particularly under high-nitrogen treatments.					
36364023	13	65	theme	fumaric	2297:2303	arg1	acid					2316:2319	lactic acid	2309:2319	lactic acid	2309:2319	Large amounts of both fumaric and lactic acid were produced, particularly under high-nitrogen treatments.					
36364023	13	65	theme	fumaric	2297:2303	arg1	amounts					2281:2287	Large amounts	2275:2287	Large amounts of both fumaric and lactic acid	2275:2319	Large amounts of both fumaric and lactic acid were produced, particularly under high-nitrogen treatments.					
36364023	14	66	theme	fumaric	2435:2441	arg1	acid					2443:2446	fumaric acid	2435:2446	fumaric acid	2435:2446	Moreover, almond shoots placed in dilute solutions of fumaric acid or lactic acid developed leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field, suggesting both acids are involved in causing disease.					
36364023	1	67	theme	simple	156:161	arg1	sugars					163:168	simple sugars	156:168	simple sugars	156:168	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	7	68	theme	higher	1451:1456	arg1	amounts					1458:1464	higher amounts	1451:1464	higher amounts than lactic acid	1451:1481	The maximum amounts of metabolites were produced when glucose was the carbon source, with fumaric acid produced in higher amounts than lactic acid.					
36364023	4	69	theme	sources	733:739	arg1	sucrose					695:701	sucrose	695:701	sucrose	695:701	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	69	theme	sources	733:739	arg1	sources					667:673	four carbon sources	655:673	four carbon sources (glucose, fructose, sucrose, and xylose)	655:714	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	69	theme	sources	733:739	arg1	sources					733:739	two nitrogen sources	720:739	two nitrogen sources (asparagine and ammonium sulphate)	720:774	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	69	theme	sources	733:739	arg1	glucose					676:682	glucose	676:682	glucose	676:682	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	69	theme	sources	733:739	arg1	xylose					708:713	xylose	708:713	xylose	708:713	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	69	theme	sources	733:739	arg1	one					648:650	one	648:650	one	648:650	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	69	theme	sources	733:739	arg1	asparagine					742:751	asparagine	742:751	asparagine	742:751	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	69	theme	sources	733:739	arg1	fructose					685:692	fructose	685:692	fructose	685:692	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	69	theme	sources	733:739	arg1	sulphate					766:773	ammonium sulphate	757:773	ammonium sulphate	757:773	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	10	70	dep	high-	1852:1856	arg1	treatments					1885:1894	treatments	1885:1894	treatments	1885:1894	Additionally, R. stolonifer isolate 19-0030 was inoculated into Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments, and hull compositions of infected and uninfected fruit were analysed using 1H NMR-based metabolomics.					
36364023	11	71	theme	water	2085:2089	arg1	treatments					2091:2100	the nitrogen and water treatments	2068:2100	the nitrogen and water treatments provided to the trees, being higher in the high-nitrogen and water treatments	2068:2178	Glucose and asparagine content of uninfected hulls was influenced by the nitrogen and water treatments provided to the trees, being higher in the high-nitrogen and water treatments.					
36364023	4	72	theme	infected	582:589	arg1	hulls					598:602	infected almond hulls	582:602	infected almond hulls	582:602	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	3	73	from	disease	425:431	arg1	almonds					436:442	almonds	436:442	almonds	436:442	Rhizopus stolonifer causes hull rot disease in almonds, symptoms of which have been previously associated with the fungus's production of fumaric acid.					
36364023	4	74	theme	almond	792:797	arg1	composition					804:814	almond hull composition	792:814	almond hull composition	792:814	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	11	75	theme	nitrogen	2072:2079	arg1	treatments					2091:2100	the nitrogen and water treatments	2068:2100	the nitrogen and water treatments provided to the trees, being higher in the high-nitrogen and water treatments	2068:2178	Glucose and asparagine content of uninfected hulls was influenced by the nitrogen and water treatments provided to the trees, being higher in the high-nitrogen and water treatments.					
36364023	13	76	theme	Large	2275:2279	arg1	fumaric					2297:2303	fumaric	2297:2303	fumaric	2297:2303	Large amounts of both fumaric and lactic acid were produced, particularly under high-nitrogen treatments.					
36364023	13	76	theme	Large	2275:2279	arg1	acid					2316:2319	lactic acid	2309:2319	lactic acid	2309:2319	Large amounts of both fumaric and lactic acid were produced, particularly under high-nitrogen treatments.					
36364023	13	76	theme	Large	2275:2279	arg1	amounts					2281:2287	Large amounts	2275:2287	Large amounts of both fumaric and lactic acid	2275:2319	Large amounts of both fumaric and lactic acid were produced, particularly under high-nitrogen treatments.					
36364023	7	77	theme	fumaric	1426:1432	arg1	acid					1434:1437	fumaric acid	1426:1437	fumaric acid produced in higher amounts than lactic acid	1426:1481	The maximum amounts of metabolites were produced when glucose was the carbon source, with fumaric acid produced in higher amounts than lactic acid.					
36364023	9	78	theme	fumaric	1730:1736	arg1	acid					1738:1741	fumaric acid	1730:1741	fumaric acid	1730:1741	This is the first report, to our knowledge, of R. stolonifer producing lactic acid in preference to fumaric acid.					
36364023	10	79	theme	stolonifer	1761:1770	arg1	isolate					1772:1778	R. stolonifer isolate 19-0030	1758:1786	R. stolonifer isolate 19-0030	1758:1786	Additionally, R. stolonifer isolate 19-0030 was inoculated into Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments, and hull compositions of infected and uninfected fruit were analysed using 1H NMR-based metabolomics.					
36364023	6	80	theme	Sugar	1136:1140	arg1	metabolisation					1142:1155	Sugar metabolisation	1136:1155	Sugar metabolisation	1136:1155	Sugar metabolisation and acid production were significantly influenced by sugar source and isolates, with five isolates depleting glucose most rapidly, followed by fructose, sucrose, and then xylose.					
36364023	1	81	theme	sources	327:333	arg1	presence					286:293	the presence	282:293	the presence of specific carbon and nitrogen sources	282:333	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	10	82	theme	fruit	1946:1950	arg1	compositions					1906:1917	hull compositions	1901:1917	hull compositions of infected and uninfected fruit	1901:1950	Additionally, R. stolonifer isolate 19-0030 was inoculated into Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments, and hull compositions of infected and uninfected fruit were analysed using 1H NMR-based metabolomics.					
36364023	4	83	theme	artificial	618:627	arg1	media					629:633	artificial media	618:633	artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition	618:814	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	7	84	theme	carbon	1406:1411	arg1	glucose					1390:1396	glucose	1390:1396	glucose	1390:1396	The maximum amounts of metabolites were produced when glucose was the carbon source, with fumaric acid produced in higher amounts than lactic acid.					
36364023	7	84	theme	carbon	1406:1411	arg1	source					1413:1418	the carbon source	1402:1418	the carbon source	1402:1418	The maximum amounts of metabolites were produced when glucose was the carbon source, with fumaric acid produced in higher amounts than lactic acid.					
36364023	14	85	theme	rot	2538:2540	arg1	disease					2542:2548	hull rot disease	2533:2548	hull rot disease in the field	2533:2561	Moreover, almond shoots placed in dilute solutions of fumaric acid or lactic acid developed leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field, suggesting both acids are involved in causing disease.					
36364023	3	86	theme	fumaric	527:533	arg1	acid					535:538	fumaric acid	527:538	fumaric acid	527:538	Rhizopus stolonifer causes hull rot disease in almonds, symptoms of which have been previously associated with the fungus's production of fumaric acid.					
36364023	8	87	theme	higher	1581:1586	arg1	amounts					1588:1594	higher amounts	1581:1594	higher amounts of lactic acid	1581:1609	Isolate 19A-0069, however, preferred sucrose as the carbon source, and Isolate 19A-0030 produced higher amounts of lactic acid than fumaric acid.					
36364023	8	87	theme	higher	1581:1586	arg1	acid					1606:1609	lactic acid	1599:1609	lactic acid	1599:1609	Isolate 19A-0069, however, preferred sucrose as the carbon source, and Isolate 19A-0030 produced higher amounts of lactic acid than fumaric acid.					
36364023	1	88	theme	genus	121:125	arg1	Rhizopus					127:134	the fungal genus Rhizopus	110:134	the fungal genus Rhizopus	110:134	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	11	89	theme	asparagine	2011:2020	arg1	content					2022:2028	Glucose and asparagine content	1999:2028	Glucose and asparagine content of uninfected hulls	1999:2048	Glucose and asparagine content of uninfected hulls was influenced by the nitrogen and water treatments provided to the trees, being higher in the high-nitrogen and water treatments.					
36364023	10	90	theme	1H	1972:1973	arg1	metabolomics					1985:1996	1H NMR-based metabolomics	1972:1996	1H NMR-based metabolomics	1972:1996	Additionally, R. stolonifer isolate 19-0030 was inoculated into Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments, and hull compositions of infected and uninfected fruit were analysed using 1H NMR-based metabolomics.					
36364023	5	91	theme	lactic	1083:1088	arg1	acid					1090:1093	lactic acid	1083:1093	lactic acid	1083:1093	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	5	91	theme	lactic	1083:1088	arg1	metabolites					1050:1060	three metabolites	1044:1060	three metabolites	1044:1060	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	0	92	theme	Almond	74:79	arg1	Disease					90:96	Almond Hull Rot Disease	74:96	Almond Hull Rot Disease	74:96	Production of Primary Metabolites by Rhizopus stolonifer, Causal Agent of Almond Hull Rot Disease.					
36364023	1	93	theme	malic	268:272	arg1	acid					274:277	malic acid	268:277	malic acid	268:277	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	7	94	with	source	1413:1418	arg1	acid					1434:1437	fumaric acid	1426:1437	fumaric acid produced in higher amounts than lactic acid	1426:1481	The maximum amounts of metabolites were produced when glucose was the carbon source, with fumaric acid produced in higher amounts than lactic acid.					
36364023	11	95	theme	Glucose	1999:2005	arg1	content					2022:2028	Glucose and asparagine content	1999:2028	Glucose and asparagine content of uninfected hulls	1999:2048	Glucose and asparagine content of uninfected hulls was influenced by the nitrogen and water treatments provided to the trees, being higher in the high-nitrogen and water treatments.					
36364023	0	96	theme	Rot	86:88	arg1	Disease					90:96	Almond Hull Rot Disease	74:96	Almond Hull Rot Disease	74:96	Production of Primary Metabolites by Rhizopus stolonifer, Causal Agent of Almond Hull Rot Disease.					
36364023	8	97	theme	acid	1606:1609	arg1	amounts					1588:1594	higher amounts	1581:1594	higher amounts of lactic acid	1581:1609	Isolate 19A-0069, however, preferred sucrose as the carbon source, and Isolate 19A-0030 produced higher amounts of lactic acid than fumaric acid.					
36364023	8	97	theme	acid	1606:1609	arg1	acid					1606:1609	lactic acid	1599:1609	lactic acid	1599:1609	Isolate 19A-0069, however, preferred sucrose as the carbon source, and Isolate 19A-0030 produced higher amounts of lactic acid than fumaric acid.					
36364023	5	98	theme	Proton	838:843	arg1	NMR					876:878	1H NMR	873:878	1H NMR	873:878	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	5	98	theme	Proton	838:843	arg1	resonance					862:870	Proton nuclear magnetic resonance	838:870	Proton nuclear magnetic resonance (1H NMR)-based metabolomics	838:898	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	11	99	theme	hulls	2044:2048	arg1	content					2022:2028	Glucose and asparagine content	1999:2028	Glucose and asparagine content of uninfected hulls	1999:2048	Glucose and asparagine content of uninfected hulls was influenced by the nitrogen and water treatments provided to the trees, being higher in the high-nitrogen and water treatments.					
36364023	4	100	theme	sources	667:673	arg1	sucrose					695:701	sucrose	695:701	sucrose	695:701	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	100	theme	sources	667:673	arg1	sources					667:673	four carbon sources	655:673	four carbon sources (glucose, fructose, sucrose, and xylose)	655:714	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	100	theme	sources	667:673	arg1	sources					733:739	two nitrogen sources	720:739	two nitrogen sources (asparagine and ammonium sulphate)	720:774	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	100	theme	sources	667:673	arg1	glucose					676:682	glucose	676:682	glucose	676:682	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	100	theme	sources	667:673	arg1	xylose					708:713	xylose	708:713	xylose	708:713	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	100	theme	sources	667:673	arg1	one					648:650	one	648:650	one	648:650	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	100	theme	sources	667:673	arg1	asparagine					742:751	asparagine	742:751	asparagine	742:751	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	100	theme	sources	667:673	arg1	fructose					685:692	fructose	685:692	fructose	685:692	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	4	100	theme	sources	667:673	arg1	sulphate					766:773	ammonium sulphate	757:773	ammonium sulphate	757:773	Six isolates of R. stolonifer taken from infected almond hulls were grown in artificial media amended with one of four carbon sources (glucose, fructose, sucrose, and xylose) and two nitrogen sources (asparagine and ammonium sulphate) chosen based on almond hull composition and used in industry.					
36364023	10	101	theme	Nonpareil	1808:1816	arg1	fruit					1825:1829	Nonpareil almond fruit	1808:1829	Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments	1808:1894	Additionally, R. stolonifer isolate 19-0030 was inoculated into Nonpareil almond fruit on trees grown under high- and low-nitrogen and water treatments, and hull compositions of infected and uninfected fruit were analysed using 1H NMR-based metabolomics.					
36364023	8	102	theme	fumaric	1616:1622	arg1	acid					1624:1627	fumaric acid	1616:1627	fumaric acid	1616:1627	Isolate 19A-0069, however, preferred sucrose as the carbon source, and Isolate 19A-0030 produced higher amounts of lactic acid than fumaric acid.					
36364023	5	103	theme	magnetic	853:860	arg1	NMR					876:878	1H NMR	873:878	1H NMR	873:878	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	5	103	theme	magnetic	853:860	arg1	resonance					862:870	Proton nuclear magnetic resonance	838:870	Proton nuclear magnetic resonance (1H NMR)-based metabolomics	838:898	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	1	104	theme	fumaric	203:209	arg1	acid					211:214	fumaric acid	203:214	fumaric acid	203:214	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	1	105	theme	specific	298:305	arg1	carbon					307:312	specific carbon and nitrogen sources	298:333	carbon	307:312	Species in the fungal genus Rhizopus are able to convert simple sugars into primary metabolites such as fumaric acid, lactic acid, citric acid, and, to a lesser extent, malic acid in the presence of specific carbon and nitrogen sources.					
36364023	0	106	theme	Metabolites	22:32	arg1	Production					0:9	Production	0:9	Production of Primary Metabolites by Rhizopus stolonifer	0:55	Production of Primary Metabolites by Rhizopus stolonifer, Causal Agent of Almond Hull Rot Disease.					
36364023	0	106	theme	Metabolites	22:32	arg1	Agent					65:69	Causal Agent	58:69	Causal Agent of Almond Hull Rot Disease	58:96	Production of Primary Metabolites by Rhizopus stolonifer, Causal Agent of Almond Hull Rot Disease.					
36364023	2	107	attach	linked	358:363	arg2	ability					341:347	This ability	336:347	This ability	336:347	This ability has been linked to plant pathogenicity.					
36364023	2	107	attach	linked	358:363	arg1	pathogenicity					374:386	plant pathogenicity	368:386	plant pathogenicity	368:386	This ability has been linked to plant pathogenicity.					
36364023	0	108	theme	Rhizopus	37:44	arg1	stolonifer					46:55	Rhizopus stolonifer	37:55	Rhizopus stolonifer	37:55	Production of Primary Metabolites by Rhizopus stolonifer, Causal Agent of Almond Hull Rot Disease.					
36364023	7	109	theme	metabolites	1359:1369	arg1	amounts					1348:1354	The maximum amounts	1336:1354	The maximum amounts of metabolites	1336:1369	The maximum amounts of metabolites were produced when glucose was the carbon source, with fumaric acid produced in higher amounts than lactic acid.					
36364023	7	109	theme	metabolites	1359:1369	arg1	metabolites					1359:1369	metabolites	1359:1369	metabolites	1359:1369	The maximum amounts of metabolites were produced when glucose was the carbon source, with fumaric acid produced in higher amounts than lactic acid.					
36364023	5	110	theme	-based	880:885	arg1	metabolomics					887:898	Proton nuclear magnetic resonance (1H NMR)-based metabolomics	838:898	Proton nuclear magnetic resonance (1H NMR)-based metabolomics	838:898	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	7	111	theme	lactic	1471:1476	arg1	acid					1478:1481	lactic acid	1471:1481	lactic acid	1471:1481	The maximum amounts of metabolites were produced when glucose was the carbon source, with fumaric acid produced in higher amounts than lactic acid.					
36364023	5	112	theme	nitrogen	1015:1022	arg1	sources					1024:1030	both nitrogen sources	1010:1030	both nitrogen sources	1010:1030	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	14	113	theme	leaf	2473:2476	arg1	symptoms					2478:2485	leaf symptoms	2473:2485	leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field	2473:2561	Moreover, almond shoots placed in dilute solutions of fumaric acid or lactic acid developed leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field, suggesting both acids are involved in causing disease.					
36364023	3	114	theme	hull	416:419	arg1	disease					425:431	hull rot disease	416:431	hull rot disease in almonds, symptoms of which have been previously associated with the fungus's production of fumaric acid	416:538	Rhizopus stolonifer causes hull rot disease in almonds, symptoms of which have been previously associated with the fungus's production of fumaric acid.					
36364023	5	115	theme	fumaric	1069:1075	arg1	acid					1077:1080	fumaric acid	1069:1080	fumaric acid	1069:1080	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	5	115	theme	fumaric	1069:1075	arg1	metabolites					1050:1060	three metabolites	1044:1060	three metabolites	1044:1060	Proton nuclear magnetic resonance (1H NMR)-based metabolomics identified that R. stolonifer could metabolise glucose, fructose, sucrose, and to a lesser extent xylose, and both nitrogen sources, to produce three metabolites, i.e., fumaric acid, lactic acid, and ethanol, under in vitro conditions.					
36364023	14	116	theme	acid	2458:2461	arg1	solutions					2422:2430	dilute solutions	2415:2430	dilute solutions of fumaric acid or lactic acid	2415:2461	Moreover, almond shoots placed in dilute solutions of fumaric acid or lactic acid developed leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field, suggesting both acids are involved in causing disease.					
36364023	12	117	theme	infected	2184:2191	arg1	hulls					2193:2197	infected hulls	2184:2197	infected hulls	2184:2197	In infected hulls, glucose and fructose were significantly reduced but not sucrose or xylose.					
36364023	14	118	from	disease	2542:2548	arg1	field					2557:2561	the field	2553:2561	the field	2553:2561	Moreover, almond shoots placed in dilute solutions of fumaric acid or lactic acid developed leaf symptoms very similar to the 'strike' symptoms seen in hull rot disease in the field, suggesting both acids are involved in causing disease.					
35660912	5	0	theme	NIR	813:815	arg1	density					829:835	NIR laser power density	813:835	NIR laser power density	813:835	Remarkably, when NIR laser power density increased to 1.0 W cm-2, the aerogels began to burn, achieving the superhigh surface temperature of ∼ 690 °C.					
35660912	5	1	theme	temperature	922:932	arg1	of ∼ 690 °C					934:944	the superhigh surface temperature of ∼ 690 °C	900:944	the superhigh surface temperature of ∼ 690 °C	900:944	Remarkably, when NIR laser power density increased to 1.0 W cm-2, the aerogels began to burn, achieving the superhigh surface temperature of ∼ 690 °C.					
35660912	7	2	from	temperature	1162:1172	arg1	future					1177:1182	future	1177:1182	future	1177:1182	Therefore, this work provides experiment evidence and theoretical basis for the rational applications of photothermal materials at high temperature in future.					
35660912	3	3	theme	conversion	529:538	arg1	capability					540:549	extraordinary light-to-heat energy conversion capability	494:549	extraordinary light-to-heat energy conversion capability	494:549	Herein, a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs) was fabricated, affording extraordinary light-to-heat energy conversion capability.					
35660912	4	4	theme	nanofibers/molybdenum	627:647	arg1	aerogels					670:677	the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels	603:677	the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels	603:677	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	5	5	theme	laser	817:821	arg1	density					829:835	NIR laser power density	813:835	NIR laser power density	813:835	Remarkably, when NIR laser power density increased to 1.0 W cm-2, the aerogels began to burn, achieving the superhigh surface temperature of ∼ 690 °C.					
35660912	7	6	theme	high	1157:1160	arg1	temperature					1162:1172	high temperature	1157:1172	high temperature in future	1157:1182	Therefore, this work provides experiment evidence and theoretical basis for the rational applications of photothermal materials at high temperature in future.					
35660912	4	7	theme	cellulose	617:625	arg1	aerogels					670:677	the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels	603:677	the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels	603:677	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	3	8	theme	aerogels	374:381	arg1	class					358:362	a class	356:362	a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs)	356:466	Herein, a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs) was fabricated, affording extraordinary light-to-heat energy conversion capability.					
35660912	4	9	dep	260.4 °C	725:732	arg1	up					719:720	up	719:720	up	719:720	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	6	10	theme	composite	982:990	arg1	aerogels					992:999	CNF/MoS2 composite aerogels	973:999	CNF/MoS2 composite aerogels	973:999	The combustion process of CNF/MoS2 composite aerogels was evaluated in detail.					
35660912	6	11	theme	combustion	951:960	arg1	process					962:968	The combustion process	947:968	The combustion process of CNF/MoS2 composite aerogels	947:999	The combustion process of CNF/MoS2 composite aerogels was evaluated in detail.					
35660912	4	12	theme	NIR	754:756	arg1	densities					771:779	near infrared (NIR) laser power densities	739:779	near infrared (NIR) laser power densities of 0.8 W cm-2	739:793	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	3	13	theme	extraordinary	494:506	arg1	capability					540:549	extraordinary light-to-heat energy conversion capability	494:549	extraordinary light-to-heat energy conversion capability	494:549	Herein, a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs) was fabricated, affording extraordinary light-to-heat energy conversion capability.					
35660912	4	14	theme	laser	759:763	arg1	densities					771:779	near infrared (NIR) laser power densities	739:779	near infrared (NIR) laser power densities of 0.8 W cm-2	739:793	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	1	15	theme	photothermal	89:100	arg1	materials					102:110	The photothermal materials	85:110	The photothermal materials	85:110	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	5	16	theme	superhigh	904:912	arg1	of ∼ 690 °C					934:944	the superhigh surface temperature of ∼ 690 °C	900:944	the superhigh surface temperature of ∼ 690 °C	900:944	Remarkably, when NIR laser power density increased to 1.0 W cm-2, the aerogels began to burn, achieving the superhigh surface temperature of ∼ 690 °C.					
35660912	3	17	theme	stable	367:372	arg1	aerogels					374:381	stable aerogels	367:381	stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs)	367:466	Herein, a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs) was fabricated, affording extraordinary light-to-heat energy conversion capability.					
35660912	4	18	theme	intercalated	572:583	arg1	structure					592:600	the intercalated porous structure	568:600	the intercalated porous structure	568:600	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	6	19	theme	aerogels	992:999	arg1	process					962:968	The combustion process	947:968	The combustion process of CNF/MoS2 composite aerogels	947:999	The combustion process of CNF/MoS2 composite aerogels was evaluated in detail.					
35660912	0	20	theme	nanofiber/molybdenum	10:29	arg1	aerogels					41:48	Cellulose nanofiber/molybdenum disulfide aerogels	0:48	Cellulose nanofiber/molybdenum disulfide aerogels for	0:52	Cellulose nanofiber/molybdenum disulfide aerogels for ultrahigh photothermal effect.					
35660912	4	21	theme	0.8 W cm-2	784:793	arg1	densities					771:779	near infrared (NIR) laser power densities	739:779	near infrared (NIR) laser power densities of 0.8 W cm-2	739:793	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	3	22	theme	cellulose	440:448	arg1	CNFs					462:465	CNFs	462:465	CNFs	462:465	Herein, a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs) was fabricated, affording extraordinary light-to-heat energy conversion capability.					
35660912	3	22	theme	cellulose	440:448	arg1	nanofibers					450:459	cellulose nanofibers	440:459	cellulose nanofibers (CNFs)	440:466	Herein, a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs) was fabricated, affording extraordinary light-to-heat energy conversion capability.					
35660912	0	23	theme	Cellulose	0:8	arg1	aerogels					41:48	Cellulose nanofiber/molybdenum disulfide aerogels	0:48	Cellulose nanofiber/molybdenum disulfide aerogels for	0:52	Cellulose nanofiber/molybdenum disulfide aerogels for ultrahigh photothermal effect.					
35660912	4	24	theme	power	765:769	arg1	densities					771:779	near infrared (NIR) laser power densities	739:779	near infrared (NIR) laser power densities of 0.8 W cm-2	739:793	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	1	25	theme	photothermal	196:207	arg1	spills					160:165	crude oil spills	150:165	crude oil spills treatment, desalination, and photothermal therapy	150:215	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	1	25	theme	photothermal	196:207	arg1	therapy					209:215	photothermal therapy	196:215	photothermal therapy	196:215	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	7	26	theme	photothermal	1131:1142	arg1	materials					1144:1152	photothermal materials	1131:1152	photothermal materials	1131:1152	Therefore, this work provides experiment evidence and theoretical basis for the rational applications of photothermal materials at high temperature in future.					
35660912	1	27	theme	broad	119:123	arg1	range					125:129	a broad range	117:129	a broad range of applications	117:145	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	4	28	theme	ultrahigh	690:698	arg1	output					712:717	an ultrahigh temperature output	687:717	an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2	687:793	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	0	29	theme	disulfide	31:39	arg1	aerogels					41:48	Cellulose nanofiber/molybdenum disulfide aerogels	0:48	Cellulose nanofiber/molybdenum disulfide aerogels for	0:52	Cellulose nanofiber/molybdenum disulfide aerogels for ultrahigh photothermal effect.					
35660912	4	30	theme	resultant	607:615	arg1	aerogels					670:677	the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels	603:677	the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels	603:677	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	7	31	theme	materials	1144:1152	arg1	applications					1115:1126	the rational applications	1102:1126	the rational applications of photothermal materials	1102:1152	Therefore, this work provides experiment evidence and theoretical basis for the rational applications of photothermal materials at high temperature in future.					
35660912	1	32	dep	spills	160:165	arg1	spills					160:165	crude oil spills	150:165	crude oil spills treatment, desalination, and photothermal therapy	150:215	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	1	32	dep	spills	160:165	arg1	desalination					178:189	desalination	178:189	desalination	178:189	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	1	32	dep	spills	160:165	arg1	treatment					167:175	treatment	167:175	treatment	167:175	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	1	32	dep	spills	160:165	arg1	therapy					209:215	photothermal therapy	196:215	photothermal therapy	196:215	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	3	33	theme	molybdenum	396:405	arg1	nanoflowers					424:434	molybdenum disulfide (MoS2) nanoflowers	396:434	molybdenum disulfide (MoS2) nanoflowers	396:434	Herein, a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs) was fabricated, affording extraordinary light-to-heat energy conversion capability.					
35660912	5	34	theme	power	823:827	arg1	density					829:835	NIR laser power density	813:835	NIR laser power density	813:835	Remarkably, when NIR laser power density increased to 1.0 W cm-2, the aerogels began to burn, achieving the superhigh surface temperature of ∼ 690 °C.					
35660912	3	35	theme	energy	522:527	arg1	capability					540:549	extraordinary light-to-heat energy conversion capability	494:549	extraordinary light-to-heat energy conversion capability	494:549	Herein, a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs) was fabricated, affording extraordinary light-to-heat energy conversion capability.					
35660912	4	36	with	260.4 °C	725:732	arg1	densities					771:779	near infrared (NIR) laser power densities	739:779	near infrared (NIR) laser power densities of 0.8 W cm-2	739:793	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	5	37	theme	surface	914:920	arg1	of ∼ 690 °C					934:944	the superhigh surface temperature of ∼ 690 °C	900:944	the superhigh surface temperature of ∼ 690 °C	900:944	Remarkably, when NIR laser power density increased to 1.0 W cm-2, the aerogels began to burn, achieving the superhigh surface temperature of ∼ 690 °C.					
35660912	4	38	theme	infrared	744:751	arg1	densities					771:779	near infrared (NIR) laser power densities	739:779	near infrared (NIR) laser power densities of 0.8 W cm-2	739:793	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	4	39	theme	temperature	700:710	arg1	output					712:717	an ultrahigh temperature output	687:717	an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2	687:793	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	3	40	theme	MoS2	418:421	arg1	nanoflowers					424:434	molybdenum disulfide (MoS2) nanoflowers	396:434	molybdenum disulfide (MoS2) nanoflowers	396:434	Herein, a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs) was fabricated, affording extraordinary light-to-heat energy conversion capability.					
35660912	2	41	with	aerogels	256:263	arg1	performance					295:305	exceptional photothermal performance	270:305	exceptional photothermal performance	270:305	However, the rational construction of aerogels with exceptional photothermal performance is highly desired yet still challenging.					
35660912	2	42	theme	rational	231:238	arg1	construction					240:251	the rational construction	227:251	the rational construction of aerogels with exceptional photothermal performance	227:305	However, the rational construction of aerogels with exceptional photothermal performance is highly desired yet still challenging.					
35660912	2	42	theme	rational	231:238	arg1	desired					317:323	desired	317:323	desired	317:323	However, the rational construction of aerogels with exceptional photothermal performance is highly desired yet still challenging.					
35660912	7	43	theme	theoretical	1080:1090	arg1	basis					1092:1096	theoretical basis	1080:1096	theoretical basis	1080:1096	Therefore, this work provides experiment evidence and theoretical basis for the rational applications of photothermal materials at high temperature in future.					
35660912	3	44	theme	light-to-heat	508:520	arg1	capability					540:549	extraordinary light-to-heat energy conversion capability	494:549	extraordinary light-to-heat energy conversion capability	494:549	Herein, a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs) was fabricated, affording extraordinary light-to-heat energy conversion capability.					
35660912	0	45	theme	photothermal	64:75	arg1	effect					77:82	photothermal effect	64:82	photothermal effect	64:82	Cellulose nanofiber/molybdenum disulfide aerogels for ultrahigh photothermal effect.					
35660912	4	46	theme	porous	585:590	arg1	structure					592:600	the intercalated porous structure	568:600	the intercalated porous structure	568:600	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	7	47	theme	rational	1106:1113	arg1	applications					1115:1126	the rational applications	1102:1126	the rational applications of photothermal materials	1102:1152	Therefore, this work provides experiment evidence and theoretical basis for the rational applications of photothermal materials at high temperature in future.					
35660912	7	48	theme	experiment	1056:1065	arg1	evidence					1067:1074	experiment evidence	1056:1074	experiment evidence	1056:1074	Therefore, this work provides experiment evidence and theoretical basis for the rational applications of photothermal materials at high temperature in future.					
35660912	1	49	theme	applications	134:145	arg1	range					125:129	a broad range	117:129	a broad range of applications	117:145	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	1	50	contain	have	112:115	arg2	range					125:129	a broad range	117:129	a broad range of applications	117:145	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	1	50	contain	have	112:115	arg1	materials					102:110	The photothermal materials	85:110	The photothermal materials	85:110	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	2	51	theme	photothermal	282:293	arg1	performance					295:305	exceptional photothermal performance	270:305	exceptional photothermal performance	270:305	However, the rational construction of aerogels with exceptional photothermal performance is highly desired yet still challenging.					
35660912	4	52	theme	near	739:742	arg1	densities					771:779	near infrared (NIR) laser power densities	739:779	near infrared (NIR) laser power densities of 0.8 W cm-2	739:793	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	2	53	theme	exceptional	270:280	arg1	performance					295:305	exceptional photothermal performance	270:305	exceptional photothermal performance	270:305	However, the rational construction of aerogels with exceptional photothermal performance is highly desired yet still challenging.					
35660912	1	54	theme	crude	150:154	arg1	spills					160:165	crude oil spills	150:165	crude oil spills treatment, desalination, and photothermal therapy	150:215	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	1	54	theme	crude	150:154	arg1	desalination					178:189	desalination	178:189	desalination	178:189	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	1	54	theme	crude	150:154	arg1	treatment					167:175	treatment	167:175	treatment	167:175	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	1	54	theme	crude	150:154	arg1	therapy					209:215	photothermal therapy	196:215	photothermal therapy	196:215	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	4	55	theme	CNF/MoS2	660:667	arg1	aerogels					670:677	the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels	603:677	the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels	603:677	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	1	56	theme	oil	156:158	arg1	spills					160:165	crude oil spills	150:165	crude oil spills treatment, desalination, and photothermal therapy	150:215	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	1	56	theme	oil	156:158	arg1	desalination					178:189	desalination	178:189	desalination	178:189	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	1	56	theme	oil	156:158	arg1	treatment					167:175	treatment	167:175	treatment	167:175	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	1	56	theme	oil	156:158	arg1	therapy					209:215	photothermal therapy	196:215	photothermal therapy	196:215	The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy.					
35660912	4	57	theme	disulfide	649:657	arg1	aerogels					670:677	the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels	603:677	the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels	603:677	Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2.					
35660912	6	58	theme	CNF/MoS2	973:980	arg1	aerogels					992:999	CNF/MoS2 composite aerogels	973:999	CNF/MoS2 composite aerogels	973:999	The combustion process of CNF/MoS2 composite aerogels was evaluated in detail.					
35660912	2	59	theme	aerogels	256:263	arg1	construction					240:251	the rational construction	227:251	the rational construction of aerogels with exceptional photothermal performance	227:305	However, the rational construction of aerogels with exceptional photothermal performance is highly desired yet still challenging.					
35660912	2	59	theme	aerogels	256:263	arg1	desired					317:323	desired	317:323	desired	317:323	However, the rational construction of aerogels with exceptional photothermal performance is highly desired yet still challenging.					
35660912	3	60	theme	disulfide	407:415	arg1	nanoflowers					424:434	molybdenum disulfide (MoS2) nanoflowers	396:434	molybdenum disulfide (MoS2) nanoflowers	396:434	Herein, a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs) was fabricated, affording extraordinary light-to-heat energy conversion capability.					
35579559	4	0	theme	light	807:811	arg1	initiators					813:822	visible light initiators	799:822	visible light initiators	799:822	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	10	1	theme	3D	1859:1860	arg1	culture					1862:1868	the 3D culture	1855:1868	the 3D culture	1855:1868	The cell-laden 10ECM@MeHA scaffold showed 94.27 ± 3.00% cell viability after 7 days of the 3D culture.					
35579559	6	2	theme	MeHA	1165:1168	arg1	hydrogel					1170:1177	the MeHA hydrogel	1161:1177	the MeHA hydrogel	1161:1177	The results showed that the mechanical property of the MeHA hydrogel is significantly improved following the addition of 10 mg mL-1 of ECM (10ECM@MeHA).					
35579559	2	3	theme	MeHA	368:371	arg1	bioink					373:378	ECM@MeHA bioink	364:378	ECM@MeHA bioink	364:378	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	2	3	theme	MeHA	368:371	arg1	bioink					356:361	a biocompatible bioink	340:361	a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties	340:451	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	6	4	theme	hydrogel	1170:1177	arg1	property					1149:1156	the mechanical property	1134:1156	the mechanical property of the MeHA hydrogel	1134:1177	The results showed that the mechanical property of the MeHA hydrogel is significantly improved following the addition of 10 mg mL-1 of ECM (10ECM@MeHA).					
35579559	7	5	theme	hydrogel	1314:1321	arg1	modulus					1292:1298	modulus	1292:1298	modulus	1292:1298	The compressive strength and modulus of 10ECM@MeHA hydrogel were 102.38 ± 5.27 kPa and 782 ± 20.36 kPa, which were 2.7 times and 3.1 times higher than those of MeHA, respectively.					
35579559	7	5	theme	hydrogel	1314:1321	arg1	strength					1279:1286	compressive strength	1267:1286	compressive strength	1267:1286	The compressive strength and modulus of 10ECM@MeHA hydrogel were 102.38 ± 5.27 kPa and 782 ± 20.36 kPa, which were 2.7 times and 3.1 times higher than those of MeHA, respectively.					
35579559	11	6	theme	MeHA	1892:1895	arg1	bioink					1897:1902	10ECM@MeHA bioink	1886:1902	10ECM@MeHA bioink	1886:1902	In conclusion, 10ECM@MeHA bioink may provide a new strategy for constructing disease models or drug screening models in vitro and is expected to be widely used in the field of tissue engineering scaffolds.					
35579559	6	7	theme	mechanical	1138:1147	arg1	property					1149:1156	the mechanical property	1134:1156	the mechanical property of the MeHA hydrogel	1134:1177	The results showed that the mechanical property of the MeHA hydrogel is significantly improved following the addition of 10 mg mL-1 of ECM (10ECM@MeHA).					
35579559	9	8	theme	10ECM	1601:1605	arg1	bioink					1612:1617	cell-laden 10ECM@MeHA bioink	1590:1617	cell-laden 10ECM@MeHA bioink	1590:1617	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	1	9	theme	desirable	211:219	arg1	environment					221:231	a desirable environment	209:231	a desirable environment for encapsulated cells	209:254	Producing a bioink that fulfills the physicochemical requirements of printing and provides a desirable environment for encapsulated cells is a major challenge in three-dimensional (3D) bioprinting.					
35579559	7	10	theme	@	1308:1308	arg1	hydrogel					1314:1321	10ECM@MeHA hydrogel	1303:1321	10ECM@MeHA hydrogel	1303:1321	The compressive strength and modulus of 10ECM@MeHA hydrogel were 102.38 ± 5.27 kPa and 782 ± 20.36 kPa, which were 2.7 times and 3.1 times higher than those of MeHA, respectively.					
35579559	11	11	theme	10ECM	1886:1890	arg1	bioink					1897:1902	10ECM@MeHA bioink	1886:1902	10ECM@MeHA bioink	1886:1902	In conclusion, 10ECM@MeHA bioink may provide a new strategy for constructing disease models or drug screening models in vitro and is expected to be widely used in the field of tissue engineering scaffolds.					
35579559	10	12	dep	viability	1829:1837	arg1	%					1822:1822	%	1822:1822	%	1822:1822	The cell-laden 10ECM@MeHA scaffold showed 94.27 ± 3.00% cell viability after 7 days of the 3D culture.					
35579559	9	13	theme	digital	1717:1723	arg1	bioprinting					1755:1765	digital light processing (DLP) -based bioprinting	1717:1765	digital light processing (DLP) -based bioprinting	1717:1765	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	9	14	theme	processing	1731:1740	arg1	bioprinting					1755:1765	digital light processing (DLP) -based bioprinting	1717:1765	digital light processing (DLP) -based bioprinting	1717:1765	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	8	15	theme	excellent	1536:1544	arg1	biocompatibility					1546:1561	excellent biocompatibility	1536:1561	excellent biocompatibility	1536:1561	After encapsulating MC3T3-E1 cells in the 10ECM@MeHA hydrogel for 7 days, the culture showed excellent biocompatibility.					
35579559	1	16	dep	three-dimensional	280:296	arg1	3D					299:300	3D	299:300	3D	299:300	Producing a bioink that fulfills the physicochemical requirements of printing and provides a desirable environment for encapsulated cells is a major challenge in three-dimensional (3D) bioprinting.					
35579559	9	17	theme	DLP	1743:1745	arg1	bioprinting					1755:1765	digital light processing (DLP) -based bioprinting	1717:1765	digital light processing (DLP) -based bioprinting	1717:1765	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	10	18	theme	@	1788:1788	arg1	scaffold					1794:1801	The cell-laden 10ECM@MeHA scaffold	1768:1801	The cell-laden 10ECM@MeHA scaffold	1768:1801	The cell-laden 10ECM@MeHA scaffold showed 94.27 ± 3.00% cell viability after 7 days of the 3D culture.					
35579559	10	19	dep	%	1822:1822	arg1	±					1816:1816	±	1816:1816	±	1816:1816	The cell-laden 10ECM@MeHA scaffold showed 94.27 ± 3.00% cell viability after 7 days of the 3D culture.					
35579559	9	20	theme	-based	1748:1753	arg1	bioprinting					1755:1765	digital light processing (DLP) -based bioprinting	1717:1765	digital light processing (DLP) -based bioprinting	1717:1765	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	10	21	theme	cell-laden	1772:1781	arg1	scaffold					1794:1801	The cell-laden 10ECM@MeHA scaffold	1768:1801	The cell-laden 10ECM@MeHA scaffold	1768:1801	The cell-laden 10ECM@MeHA scaffold showed 94.27 ± 3.00% cell viability after 7 days of the 3D culture.					
35579559	3	22	theme	methacrylated	587:599	arg1	MeHA					618:621	MeHA	618:621	MeHA	618:621	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	3	22	theme	methacrylated	587:599	arg1	acid					612:615	methacrylated hyaluronic acid	587:615	the prepared methacrylated hyaluronic acid (MeHA)	574:622	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	4	23	theme	nm	840:841	arg1	light					851:855	515 nm visible light	836:855	515 nm visible light for 30 s	836:864	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	10	24	theme	94.27	1810:1814	arg1	±					1816:1816	±	1816:1816	±	1816:1816	The cell-laden 10ECM@MeHA scaffold showed 94.27 ± 3.00% cell viability after 7 days of the 3D culture.					
35579559	1	25	from	challenge	267:275	arg1	bioprinting					303:313	three-dimensional (3D) bioprinting	280:313	three-dimensional (3D) bioprinting	280:313	Producing a bioink that fulfills the physicochemical requirements of printing and provides a desirable environment for encapsulated cells is a major challenge in three-dimensional (3D) bioprinting.					
35579559	5	26	theme	mechanical	949:958	arg1	properties					960:969	mechanical properties	949:969	mechanical properties	949:969	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	11	27	theme	drug	1966:1969	arg1	models					1981:1986	drug screening models	1966:1986	drug screening models	1966:1986	In conclusion, 10ECM@MeHA bioink may provide a new strategy for constructing disease models or drug screening models in vitro and is expected to be widely used in the field of tissue engineering scaffolds.					
35579559	6	28	theme	@	1255:1255	arg1	ECM					1245:1247	ECM	1245:1247	ECM (10ECM@MeHA)	1245:1260	The results showed that the mechanical property of the MeHA hydrogel is significantly improved following the addition of 10 mg mL-1 of ECM (10ECM@MeHA).					
35579559	6	28	theme	@	1255:1255	arg1	MeHA					1256:1259	10ECM@MeHA	1250:1259	10ECM@MeHA	1250:1259	The results showed that the mechanical property of the MeHA hydrogel is significantly improved following the addition of 10 mg mL-1 of ECM (10ECM@MeHA).					
35579559	1	29	theme	printing	187:194	arg1	requirements					171:182	the physicochemical requirements	151:182	the physicochemical requirements of printing	151:194	Producing a bioink that fulfills the physicochemical requirements of printing and provides a desirable environment for encapsulated cells is a major challenge in three-dimensional (3D) bioprinting.					
35579559	10	30	theme	cell	1824:1827	arg1	viability					1829:1837	94.27 ± 3.00% cell viability	1810:1837	94.27 ± 3.00% cell viability	1810:1837	The cell-laden 10ECM@MeHA scaffold showed 94.27 ± 3.00% cell viability after 7 days of the 3D culture.					
35579559	0	31	theme	bioink	110:115	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink.					
35579559	0	31	theme	bioink	110:115	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink.					
35579559	9	32	theme	@	1573:1573	arg1	bioink					1579:1584	The 10ECM@MeHA bioink	1564:1584	The 10ECM@MeHA bioink	1564:1584	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	10	33	theme	3.00	1818:1821	arg1	±					1816:1816	±	1816:1816	±	1816:1816	The cell-laden 10ECM@MeHA scaffold showed 94.27 ± 3.00% cell viability after 7 days of the 3D culture.					
35579559	5	34	theme	@	998:998	arg1	hydrogel					1004:1011	ECM@MeHA hydrogel	995:1011	ECM@MeHA hydrogel	995:1011	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	2	35	theme	printable	432:440	arg1	properties					442:451	printable properties	432:451	printable properties	432:451	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	4	36	theme	@	731:731	arg1	bioink					737:742	the ECM@MeHA bioink	724:742	the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators	724:822	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	6	37	theme	mg	1234:1235	arg1	mL-1					1237:1240	10 mg mL-1	1231:1240	10 mg mL-1 of ECM (10ECM@MeHA)	1231:1260	The results showed that the mechanical property of the MeHA hydrogel is significantly improved following the addition of 10 mg mL-1 of ECM (10ECM@MeHA).					
35579559	5	38	theme	hydrogel	1004:1011	arg1	viability					1048:1056	the cell viability	1039:1056	the cell viability	1039:1056	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	5	38	theme	hydrogel	1004:1011	arg1	biocompatibility					975:990	biocompatibility	975:990	biocompatibility	975:990	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	5	38	theme	hydrogel	1004:1011	arg1	properties					960:969	mechanical properties	949:969	mechanical properties	949:969	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	5	38	theme	hydrogel	1004:1011	arg1	printability					1022:1033	the printability	1018:1033	the printability	1018:1033	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	9	39	theme	cell-laden	1590:1599	arg1	bioink					1612:1617	cell-laden 10ECM@MeHA bioink	1590:1617	cell-laden 10ECM@MeHA bioink	1590:1617	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	11	40	theme	new	1918:1920	arg1	strategy					1922:1929	a new strategy	1916:1929	a new strategy for constructing disease models or drug screening models in vitro	1916:1995	In conclusion, 10ECM@MeHA bioink may provide a new strategy for constructing disease models or drug screening models in vitro and is expected to be widely used in the field of tissue engineering scaffolds.					
35579559	2	41	theme	mechanical	395:404	arg1	support					406:412	suitable mechanical support	386:412	suitable mechanical support	386:412	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	3	42	dep	initiators	681:690	arg1	TEOA					702:705	TEOA	702:705	TEOA	702:705	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	3	42	dep	initiators	681:690	arg1	NVP					712:714	NVP	712:714	NVP	712:714	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	3	42	dep	initiators	681:690	arg1	Y					699:699	Eosin Y	693:699	Eosin Y	693:699	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	3	42	dep	initiators	681:690	arg1	initiators					681:690	the biosafe initiators	669:690	the biosafe initiators (Eosin Y, TEOA, and NVP)	669:715	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	9	43	theme	@	1606:1606	arg1	bioink					1612:1617	cell-laden 10ECM@MeHA bioink	1590:1617	cell-laden 10ECM@MeHA bioink	1590:1617	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	5	44	theme	@	1068:1068	arg1	bioink					1074:1079	the ECM@MeHA bioink	1061:1079	the ECM@MeHA bioink	1061:1079	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	3	45	theme	visible	649:655	arg1	light					657:661	visible light	649:661	visible light using the biosafe initiators (Eosin Y, TEOA, and NVP)	649:715	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	4	46	theme	mix	759:761	arg1	solution					763:770	a mix solution	757:770	a mix solution of ECM and MeHA containing visible light initiators	757:822	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	0	47	theme	photocurable	36:47	arg1	bioink					110:115	photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink	36:115	photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink	36:115	Preparation and characterization of photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink.					
35579559	4	48	attach	crosslinked	883:893	arg3	°C					901:902	37 °C	898:902	37 °C	898:902	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	4	49	theme	double	914:919	arg1	hydrogel					929:936	a double network hydrogel	912:936	a double network hydrogel	912:936	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	0	50	theme	hyaluronic	94:103	arg1	bioink					110:115	photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink	36:115	photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink	36:115	Preparation and characterization of photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink.					
35579559	11	51	theme	@	1891:1891	arg1	bioink					1897:1902	10ECM@MeHA bioink	1886:1902	10ECM@MeHA bioink	1886:1902	In conclusion, 10ECM@MeHA bioink may provide a new strategy for constructing disease models or drug screening models in vitro and is expected to be widely used in the field of tissue engineering scaffolds.					
35579559	2	52	theme	@	367:367	arg1	bioink					373:378	ECM@MeHA bioink	364:378	ECM@MeHA bioink	364:378	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	2	52	theme	@	367:367	arg1	bioink					356:361	a biocompatible bioink	340:361	a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties	340:451	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	0	53	theme	extracellular	59:71	arg1	bioink					110:115	photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink	36:115	photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink	36:115	Preparation and characterization of photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink.					
35579559	11	54	theme	tissue	2047:2052	arg1	scaffolds					2066:2074	tissue engineering scaffolds	2047:2074	tissue engineering scaffolds	2047:2074	In conclusion, 10ECM@MeHA bioink may provide a new strategy for constructing disease models or drug screening models in vitro and is expected to be widely used in the field of tissue engineering scaffolds.					
35579559	3	55	theme	biosafe	673:679	arg1	TEOA					702:705	TEOA	702:705	TEOA	702:705	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	3	55	theme	biosafe	673:679	arg1	initiators					681:690	the biosafe initiators	669:690	the biosafe initiators (Eosin Y, TEOA, and NVP)	669:715	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	3	55	theme	biosafe	673:679	arg1	Y					699:699	Eosin Y	693:699	Eosin Y	693:699	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	3	55	theme	biosafe	673:679	arg1	NVP					712:714	NVP	712:714	NVP	712:714	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	11	56	used	used	2026:2029	arg2	bioink					1897:1902	10ECM@MeHA bioink	1886:1902	10ECM@MeHA bioink	1886:1902	In conclusion, 10ECM@MeHA bioink may provide a new strategy for constructing disease models or drug screening models in vitro and is expected to be widely used in the field of tissue engineering scaffolds.					
35579559	3	57	theme	extracellular	497:509	arg1	ECM					519:521	ECM	519:521	ECM	519:521	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	3	57	theme	extracellular	497:509	arg1	matrix					511:516	the thermosensitive extracellular matrix	477:516	the thermosensitive extracellular matrix (ECM)	477:522	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	8	58	theme	MC3T3-E1	1463:1470	arg1	cells					1472:1476	MC3T3-E1 cells	1463:1476	MC3T3-E1 cells	1463:1476	After encapsulating MC3T3-E1 cells in the 10ECM@MeHA hydrogel for 7 days, the culture showed excellent biocompatibility.					
35579559	1	59	theme	major	261:265	arg1	challenge					267:275	a major challenge	259:275	a major challenge in three-dimensional (3D) bioprinting	259:313	Producing a bioink that fulfills the physicochemical requirements of printing and provides a desirable environment for encapsulated cells is a major challenge in three-dimensional (3D) bioprinting.					
35579559	7	60	theme	±	1335:1335	arg1	kPa					1342:1344	102.38 ± 5.27 kPa	1328:1344	102.38 ± 5.27 kPa	1328:1344	The compressive strength and modulus of 10ECM@MeHA hydrogel were 102.38 ± 5.27 kPa and 782 ± 20.36 kPa, which were 2.7 times and 3.1 times higher than those of MeHA, respectively.					
35579559	10	61	theme	culture	1862:1868	arg1	days					1847:1850	7 days	1845:1850	7 days of the 3D culture	1845:1868	The cell-laden 10ECM@MeHA scaffold showed 94.27 ± 3.00% cell viability after 7 days of the 3D culture.					
35579559	7	62	dep	kPa	1342:1344	arg1	times					1382:1386	2.7 times	1378:1386	2.7 times	1378:1386	The compressive strength and modulus of 10ECM@MeHA hydrogel were 102.38 ± 5.27 kPa and 782 ± 20.36 kPa, which were 2.7 times and 3.1 times higher than those of MeHA, respectively.					
35579559	7	62	dep	kPa	1342:1344	arg1	times					1396:1400	3.1 times	1392:1400	3.1 times higher than those of MeHA	1392:1426	The compressive strength and modulus of 10ECM@MeHA hydrogel were 102.38 ± 5.27 kPa and 782 ± 20.36 kPa, which were 2.7 times and 3.1 times higher than those of MeHA, respectively.					
35579559	8	63	theme	10ECM	1485:1489	arg1	hydrogel					1496:1503	the 10ECM@MeHA hydrogel	1481:1503	the 10ECM@MeHA hydrogel for 7 days	1481:1514	After encapsulating MC3T3-E1 cells in the 10ECM@MeHA hydrogel for 7 days, the culture showed excellent biocompatibility.					
35579559	5	64	theme	bioink	1074:1079	arg1	viability					1048:1056	the cell viability	1039:1056	the cell viability	1039:1056	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	5	64	theme	bioink	1074:1079	arg1	biocompatibility					975:990	biocompatibility	975:990	biocompatibility	975:990	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	5	64	theme	bioink	1074:1079	arg1	properties					960:969	mechanical properties	949:969	mechanical properties	949:969	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	5	64	theme	bioink	1074:1079	arg1	printability					1022:1033	the printability	1018:1033	the printability	1018:1033	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	4	65	theme	visible	799:805	arg1	initiators					813:822	visible light initiators	799:822	visible light initiators	799:822	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	11	66	theme	scaffolds	2066:2074	arg1	field					2038:2042	the field	2034:2042	the field of tissue engineering scaffolds	2034:2074	In conclusion, 10ECM@MeHA bioink may provide a new strategy for constructing disease models or drug screening models in vitro and is expected to be widely used in the field of tissue engineering scaffolds.					
35579559	5	67	dep	properties	960:969	arg1	the					945:947	the	945:947	the	945:947	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	8	68	theme	MeHA	1491:1494	arg1	hydrogel					1496:1503	the 10ECM@MeHA hydrogel	1481:1503	the 10ECM@MeHA hydrogel for 7 days	1481:1514	After encapsulating MC3T3-E1 cells in the 10ECM@MeHA hydrogel for 7 days, the culture showed excellent biocompatibility.					
35579559	7	69	theme	MeHA	1309:1312	arg1	hydrogel					1314:1321	10ECM@MeHA hydrogel	1303:1321	10ECM@MeHA hydrogel	1303:1321	The compressive strength and modulus of 10ECM@MeHA hydrogel were 102.38 ± 5.27 kPa and 782 ± 20.36 kPa, which were 2.7 times and 3.1 times higher than those of MeHA, respectively.					
35579559	9	70	theme	extrusion-based	1685:1699	arg1	bioprinting					1701:1711	the extrusion-based bioprinting	1681:1711	the extrusion-based bioprinting	1681:1711	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	7	71	theme	10ECM	1303:1307	arg1	hydrogel					1314:1321	10ECM@MeHA hydrogel	1303:1321	10ECM@MeHA hydrogel	1303:1321	The compressive strength and modulus of 10ECM@MeHA hydrogel were 102.38 ± 5.27 kPa and 782 ± 20.36 kPa, which were 2.7 times and 3.1 times higher than those of MeHA, respectively.					
35579559	4	72	theme	ECM	775:777	arg1	solution					763:770	a mix solution	757:770	a mix solution of ECM and MeHA containing visible light initiators	757:822	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	4	73	dep	cure	828:831	arg1	crosslinked					883:893	crosslinked	883:893	crosslinked at 37 °C to form a double network hydrogel	883:936	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	2	74	theme	biocompatible	342:354	arg1	bioink					373:378	ECM@MeHA bioink	364:378	ECM@MeHA bioink	364:378	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	2	74	theme	biocompatible	342:354	arg1	bioink					356:361	a biocompatible bioink	340:361	a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties	340:451	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	4	75	theme	MeHA	783:786	arg1	solution					763:770	a mix solution	757:770	a mix solution of ECM and MeHA containing visible light initiators	757:822	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	7	76	theme	compressive	1267:1277	arg1	strength					1279:1286	compressive strength	1267:1286	compressive strength	1267:1286	The compressive strength and modulus of 10ECM@MeHA hydrogel were 102.38 ± 5.27 kPa and 782 ± 20.36 kPa, which were 2.7 times and 3.1 times higher than those of MeHA, respectively.					
35579559	9	77	theme	light	1725:1729	arg1	bioprinting					1755:1765	digital light processing (DLP) -based bioprinting	1717:1765	digital light processing (DLP) -based bioprinting	1717:1765	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	6	78	theme	10	1231:1232	arg1	mg					1234:1235	mg	1234:1235	mg	1234:1235	The results showed that the mechanical property of the MeHA hydrogel is significantly improved following the addition of 10 mg mL-1 of ECM (10ECM@MeHA).					
35579559	1	79	theme	encapsulated	237:248	arg1	cells					250:254	encapsulated cells	237:254	encapsulated cells	237:254	Producing a bioink that fulfills the physicochemical requirements of printing and provides a desirable environment for encapsulated cells is a major challenge in three-dimensional (3D) bioprinting.					
35579559	4	80	theme	visible	843:849	arg1	light					851:855	515 nm visible light	836:855	515 nm visible light for 30 s	836:864	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	3	81	theme	hyaluronic	601:610	arg1	MeHA					618:621	MeHA	618:621	MeHA	618:621	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	3	81	theme	hyaluronic	601:610	arg1	acid					612:615	methacrylated hyaluronic acid	587:615	the prepared methacrylated hyaluronic acid (MeHA)	574:622	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	10	82	theme	10ECM	1783:1787	arg1	scaffold					1794:1801	The cell-laden 10ECM@MeHA scaffold	1768:1801	The cell-laden 10ECM@MeHA scaffold	1768:1801	The cell-laden 10ECM@MeHA scaffold showed 94.27 ± 3.00% cell viability after 7 days of the 3D culture.					
35579559	5	83	theme	ECM	995:997	arg1	hydrogel					1004:1011	ECM@MeHA hydrogel	995:1011	ECM@MeHA hydrogel	995:1011	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	3	84	theme	prepared	578:585	arg1	MeHA					618:621	MeHA	618:621	MeHA	618:621	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	3	84	theme	prepared	578:585	arg1	acid					612:615	methacrylated hyaluronic acid	587:615	the prepared methacrylated hyaluronic acid (MeHA)	574:622	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	7	85	dep	strength	1279:1286	arg1	The					1263:1265	The	1263:1265	The	1263:1265	The compressive strength and modulus of 10ECM@MeHA hydrogel were 102.38 ± 5.27 kPa and 782 ± 20.36 kPa, which were 2.7 times and 3.1 times higher than those of MeHA, respectively.					
35579559	1	86	theme	physicochemical	155:169	arg1	requirements					171:182	the physicochemical requirements	151:182	the physicochemical requirements of printing	151:194	Producing a bioink that fulfills the physicochemical requirements of printing and provides a desirable environment for encapsulated cells is a major challenge in three-dimensional (3D) bioprinting.					
35579559	4	87	contain	containing	788:797	arg2	initiators					813:822	visible light initiators	799:822	visible light initiators	799:822	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	4	87	contain	containing	788:797	arg1	solution					763:770	a mix solution	757:770	a mix solution of ECM and MeHA containing visible light initiators	757:822	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	7	88	theme	higher	1402:1407	arg1	times					1396:1400	3.1 times	1392:1400	3.1 times higher than those of MeHA	1392:1426	The compressive strength and modulus of 10ECM@MeHA hydrogel were 102.38 ± 5.27 kPa and 782 ± 20.36 kPa, which were 2.7 times and 3.1 times higher than those of MeHA, respectively.					
35579559	4	89	theme	515	836:838	arg1	nm					840:841	nm	840:841	nm	840:841	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	0	90	theme	acid	105:108	arg1	bioink					110:115	photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink	36:115	photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink	36:115	Preparation and characterization of photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink.					
35579559	11	91	theme	screening	1971:1979	arg1	models					1981:1986	drug screening models	1966:1986	drug screening models	1966:1986	In conclusion, 10ECM@MeHA bioink may provide a new strategy for constructing disease models or drug screening models in vitro and is expected to be widely used in the field of tissue engineering scaffolds.					
35579559	10	92	theme	MeHA	1789:1792	arg1	scaffold					1794:1801	The cell-laden 10ECM@MeHA scaffold	1768:1801	The cell-laden 10ECM@MeHA scaffold	1768:1801	The cell-laden 10ECM@MeHA scaffold showed 94.27 ± 3.00% cell viability after 7 days of the 3D culture.					
35579559	6	93	theme	10ECM	1250:1254	arg1	ECM					1245:1247	ECM	1245:1247	ECM (10ECM@MeHA)	1245:1260	The results showed that the mechanical property of the MeHA hydrogel is significantly improved following the addition of 10 mg mL-1 of ECM (10ECM@MeHA).					
35579559	6	93	theme	10ECM	1250:1254	arg1	MeHA					1256:1259	10ECM@MeHA	1250:1259	10ECM@MeHA	1250:1259	The results showed that the mechanical property of the MeHA hydrogel is significantly improved following the addition of 10 mg mL-1 of ECM (10ECM@MeHA).					
35579559	9	94	theme	10ECM	1568:1572	arg1	bioink					1579:1584	The 10ECM@MeHA bioink	1564:1584	The 10ECM@MeHA bioink	1564:1584	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	6	95	theme	ECM	1245:1247	arg1	mL-1					1237:1240	10 mg mL-1	1231:1240	10 mg mL-1 of ECM (10ECM@MeHA)	1231:1260	The results showed that the mechanical property of the MeHA hydrogel is significantly improved following the addition of 10 mg mL-1 of ECM (10ECM@MeHA).					
35579559	9	96	theme	MeHA	1574:1577	arg1	bioink					1579:1584	The 10ECM@MeHA bioink	1564:1584	The 10ECM@MeHA bioink	1564:1584	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	6	97	theme	mL-1	1237:1240	arg1	addition					1219:1226	the addition	1215:1226	the addition of 10 mg mL-1 of ECM (10ECM@MeHA)	1215:1260	The results showed that the mechanical property of the MeHA hydrogel is significantly improved following the addition of 10 mg mL-1 of ECM (10ECM@MeHA).					
35579559	5	98	theme	MeHA	999:1002	arg1	hydrogel					1004:1011	ECM@MeHA hydrogel	995:1011	ECM@MeHA hydrogel	995:1011	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	4	99	theme	ECM	728:730	arg1	bioink					737:742	the ECM@MeHA bioink	724:742	the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators	724:822	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	3	100	theme	bioink	462:467	arg1	system					469:474	this bioink system	457:474	this bioink system	457:474	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	4	101	theme	network	921:927	arg1	hydrogel					929:936	a double network hydrogel	912:936	a double network hydrogel	912:936	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	2	102	theme	suitable	386:393	arg1	support					406:412	suitable mechanical support	386:412	suitable mechanical support	386:412	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	5	103	theme	ECM	1065:1067	arg1	bioink					1074:1079	the ECM@MeHA bioink	1061:1079	the ECM@MeHA bioink	1061:1079	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	3	104	attach	crosslinked	548:558	arg3	°C.					566:568	37 °C.	563:568	37 °C.	563:568	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	9	105	theme	MeHA	1607:1610	arg1	bioink					1612:1617	cell-laden 10ECM@MeHA bioink	1590:1617	cell-laden 10ECM@MeHA bioink	1590:1617	The 10ECM@MeHA bioink and cell-laden 10ECM@MeHA bioink were printed, and the cells were successfully encapsulated by the extrusion-based bioprinting and digital light processing (DLP) -based bioprinting.					
35579559	3	106	theme	thermosensitive	481:495	arg1	ECM					519:521	ECM	519:521	ECM	519:521	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	3	106	theme	thermosensitive	481:495	arg1	matrix					511:516	the thermosensitive extracellular matrix	477:516	the thermosensitive extracellular matrix (ECM)	477:522	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	0	107	theme	composite	49:57	arg1	bioink					110:115	photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink	36:115	photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink	36:115	Preparation and characterization of photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink.					
35579559	5	108	theme	MeHA	1069:1072	arg1	bioink					1074:1079	the ECM@MeHA bioink	1061:1079	the ECM@MeHA bioink	1061:1079	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	2	109	with	bioink	356:361	arg1	support					406:412	suitable mechanical support	386:412	suitable mechanical support	386:412	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	2	109	with	bioink	356:361	arg1	light					426:430	visible light	418:430	visible light	418:430	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	11	110	theme	disease	1948:1954	arg1	models					1956:1961	disease models	1948:1961	disease models	1948:1961	In conclusion, 10ECM@MeHA bioink may provide a new strategy for constructing disease models or drug screening models in vitro and is expected to be widely used in the field of tissue engineering scaffolds.					
35579559	2	111	theme	visible	418:424	arg1	light					426:430	visible light	418:430	visible light	418:430	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	0	112	theme	matrix-methacrylated	73:92	arg1	bioink					110:115	photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink	36:115	photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink	36:115	Preparation and characterization of photocurable composite extracellular matrix-methacrylated hyaluronic acid bioink.					
35579559	5	113	theme	cell	1043:1046	arg1	viability					1048:1056	the cell viability	1039:1056	the cell viability	1039:1056	Then, the mechanical properties and biocompatibility of ECM@MeHA hydrogel, and the printability and the cell viability of the ECM@MeHA bioink were systemically evaluated.					
35579559	2	114	theme	ECM	364:366	arg1	bioink					373:378	ECM@MeHA bioink	364:378	ECM@MeHA bioink	364:378	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	2	114	theme	ECM	364:366	arg1	bioink					356:361	a biocompatible bioink	340:361	a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties	340:451	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	7	115	theme	±	1354:1354	arg1	kPa					1362:1364	782 ± 20.36 kPa	1350:1364	782 ± 20.36 kPa	1350:1364	The compressive strength and modulus of 10ECM@MeHA hydrogel were 102.38 ± 5.27 kPa and 782 ± 20.36 kPa, which were 2.7 times and 3.1 times higher than those of MeHA, respectively.					
35579559	11	116	theme	engineering	2054:2064	arg1	scaffolds					2066:2074	tissue engineering scaffolds	2047:2074	tissue engineering scaffolds	2047:2074	In conclusion, 10ECM@MeHA bioink may provide a new strategy for constructing disease models or drug screening models in vitro and is expected to be widely used in the field of tissue engineering scaffolds.					
35579559	4	117	theme	MeHA	732:735	arg1	bioink					737:742	the ECM@MeHA bioink	724:742	the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators	724:822	Thus, the ECM@MeHA bioink consisted of a mix solution of ECM and MeHA containing visible light initiators can cure at 515 nm visible light for 30 s and then further crosslinked at 37 °C to form a double network hydrogel.					
35579559	3	118	theme	Eosin	693:697	arg1	initiators					681:690	the biosafe initiators	669:690	the biosafe initiators (Eosin Y, TEOA, and NVP)	669:715	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	3	118	theme	Eosin	693:697	arg1	Y					699:699	Eosin Y	693:699	Eosin Y	693:699	In this bioink system, the thermosensitive extracellular matrix (ECM) was prepared and can be crosslinked at 37 °C. And the prepared methacrylated hyaluronic acid (MeHA) can be crosslinked under visible light using the biosafe initiators (Eosin Y, TEOA, and NVP).					
35579559	2	119	dep	support	406:412	arg1	properties					442:451	printable properties	432:451	printable properties	432:451	Thus, we have developed a biocompatible bioink (ECM@MeHA bioink) with suitable mechanical support and visible light printable properties.					
35579559	8	120	theme	@	1490:1490	arg1	hydrogel					1496:1503	the 10ECM@MeHA hydrogel	1481:1503	the 10ECM@MeHA hydrogel for 7 days	1481:1514	After encapsulating MC3T3-E1 cells in the 10ECM@MeHA hydrogel for 7 days, the culture showed excellent biocompatibility.					
35579559	1	121	theme	three-dimensional	280:296	arg1	bioprinting					303:313	three-dimensional (3D) bioprinting	280:313	three-dimensional (3D) bioprinting	280:313	Producing a bioink that fulfills the physicochemical requirements of printing and provides a desirable environment for encapsulated cells is a major challenge in three-dimensional (3D) bioprinting.					
37316088	9	0	theme	B.	1340:1341	arg1	PT4					1350:1352	B. longum PT4	1340:1352	B. longum PT4	1340:1352	In the presence of B. ovatus, B. longum PT4 increased the production of enzymes such as α-L-arabinosidase, L-arabinose isomerase, xylulose kinase, xylose isomerase, and sugar transporters.					
37316088	3	1	theme	fermenting	501:510	arg1	bacteria					464:471	certain gut bacteria	452:471	certain gut bacteria	452:471	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	3	1	theme	fermenting	501:510	arg1	fibers					520:525	fermenting dietary fibers	501:525	fermenting dietary fibers	501:525	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	2	2	from	fiber	328:332	arg1	rich					312:315	rich	312:315	rich	312:315	Its composition can be modulated by consuming foods rich in dietary fiber, such as xylan, a complex polysaccharide that can be considered an emerging prebiotic.					
37316088	0	3	theme	bidirectional	77:89	arg1	culture					91:97	xylan bidirectional culture	71:97	xylan bidirectional culture	71:97	Proteomic analyses of Bacteroides ovatus and Bifidobacterium longum in xylan bidirectional culture shows sugar cross-feeding interactions.					
37316088	0	4	from	analyses	10:17	arg1	culture					91:97	xylan bidirectional culture	71:97	xylan bidirectional culture	71:97	Proteomic analyses of Bacteroides ovatus and Bifidobacterium longum in xylan bidirectional culture shows sugar cross-feeding interactions.					
37316088	7	5	theme	Proteomic	1004:1012	arg1	analyses					1014:1021	Proteomic analyses	1004:1021	Proteomic analyses	1004:1021	Proteomic analyses indicated that B. ovatus HM222 synthesizes enzymes facilitating xylan degradation, such as β-xylanase, arabinosidase, L-arabinose isomerase, and xylosidase.					
37316088	3	6	theme	dietary	512:518	arg1	bacteria					464:471	certain gut bacteria	452:471	certain gut bacteria	452:471	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	3	6	theme	dietary	512:518	arg1	fibers					520:525	fermenting dietary fibers	501:525	fermenting dietary fibers	501:525	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	11	7	dep	release	1640:1646	arg1	arabinose					1698:1706	arabinose	1698:1706	arabinose	1698:1706	Bacteroides degraded this substrate to release xylooligosaccharides, or monosaccharides (xylose, arabinose), which might support the growth of secondary degraders such as B. longum.					
37316088	11	7	dep	release	1640:1646	arg1	xylose					1690:1695	xylose	1690:1695	xylose	1690:1695	Bacteroides degraded this substrate to release xylooligosaccharides, or monosaccharides (xylose, arabinose), which might support the growth of secondary degraders such as B. longum.					
37316088	8	8	theme	PT4	1305:1307	arg1	presence					1270:1277	the presence	1266:1277	the presence of Bifidobacterium longum PT4	1266:1307	Interestingly, the relative abundance of these proteins remains largely unaffected in the presence of Bifidobacterium longum PT4.					
37316088	0	9	theme	sugar	105:109	arg1	interactions					125:136	sugar cross-feeding interactions	105:136	sugar cross-feeding interactions	105:136	Proteomic analyses of Bacteroides ovatus and Bifidobacterium longum in xylan bidirectional culture shows sugar cross-feeding interactions.					
37316088	2	10	theme	dietary	320:326	arg1	fiber					328:332	dietary fiber	320:332	dietary fiber	320:332	Its composition can be modulated by consuming foods rich in dietary fiber, such as xylan, a complex polysaccharide that can be considered an emerging prebiotic.					
37316088	4	11	theme	bacterial	601:609	arg1	strains					611:617	Different bacterial strains	591:617	Different bacterial strains of Lactobacillus, Bifidobacterium, and Bacteroides	591:668	Different bacterial strains of Lactobacillus, Bifidobacterium, and Bacteroides were evaluated for their ability to consume xylan and interact with one another.					
37316088	9	12	theme	L-arabinose	1417:1427	arg1	isomerase					1429:1437	L-arabinose isomerase	1417:1437	L-arabinose isomerase	1417:1437	In the presence of B. ovatus, B. longum PT4 increased the production of enzymes such as α-L-arabinosidase, L-arabinose isomerase, xylulose kinase, xylose isomerase, and sugar transporters.					
37316088	3	13	theme	primary	482:488	arg1	degraders					490:498	primary degraders	482:498	primary degraders	482:498	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	3	13	theme	primary	482:488	arg1	bacteria					464:471	certain gut bacteria	452:471	certain gut bacteria	452:471	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	11	14	theme	secondary	1744:1752	arg1	degraders					1754:1762	secondary degraders	1744:1762	secondary degraders such as B. longum	1744:1780	Bacteroides degraded this substrate to release xylooligosaccharides, or monosaccharides (xylose, arabinose), which might support the growth of secondary degraders such as B. longum.					
37316088	11	14	theme	secondary	1744:1752	arg1	longum					1775:1780	B. longum	1772:1780	B. longum	1772:1780	Bacteroides degraded this substrate to release xylooligosaccharides, or monosaccharides (xylose, arabinose), which might support the growth of secondary degraders such as B. longum.					
37316088	5	15	theme	carbon	863:868	arg1	source					870:875	a carbon source	861:875	a carbon source	861:875	Results from unidirectional assays gave indications of possible cross-feeding between bacteria using xylan as a carbon source.					
37316088	5	15	theme	carbon	863:868	arg1	xylan					852:856	xylan	852:856	xylan	852:856	Results from unidirectional assays gave indications of possible cross-feeding between bacteria using xylan as a carbon source.					
37316088	2	16	theme	rich	312:315	arg1	foods					306:310	foods	306:310	foods rich in dietary fiber	306:332	Its composition can be modulated by consuming foods rich in dietary fiber, such as xylan, a complex polysaccharide that can be considered an emerging prebiotic.					
37316088	11	17	dep	degraded	1613:1620	arg1	support					1722:1728	support	1722:1728	might support the growth of secondary degraders such as B. longum	1716:1780	Bacteroides degraded this substrate to release xylooligosaccharides, or monosaccharides (xylose, arabinose), which might support the growth of secondary degraders such as B. longum.					
37316088	1	18	theme	anaerobic	183:191	arg1	microorganisms					193:206	anaerobic microorganisms	183:206	anaerobic microorganisms	183:206	The intestinal microbiome is a community of anaerobic microorganisms whose activities significantly impact human health.					
37316088	0	19	theme	cross-feeding	111:123	arg1	interactions					125:136	sugar cross-feeding interactions	105:136	sugar cross-feeding interactions	105:136	Proteomic analyses of Bacteroides ovatus and Bifidobacterium longum in xylan bidirectional culture shows sugar cross-feeding interactions.					
37316088	6	20	theme	longum	927:932	arg1	PT4					934:936	Bifidobacterium longum PT4	911:936	Bifidobacterium longum PT4	911:936	Bidirectional assays showed that Bifidobacterium longum PT4 increased its growth in the presence of Bacteroides ovatus HM222.					
37316088	9	21	theme	ovatus	1332:1337	arg1	presence					1317:1324	the presence	1313:1324	the presence of B. ovatus	1313:1337	In the presence of B. ovatus, B. longum PT4 increased the production of enzymes such as α-L-arabinosidase, L-arabinose isomerase, xylulose kinase, xylose isomerase, and sugar transporters.					
37316088	3	22	theme	releasing	532:540	arg1	metabolites					542:552	releasing metabolites	532:552	releasing metabolites	532:552	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	3	22	theme	releasing	532:540	arg1	bacteria					464:471	certain gut bacteria	452:471	certain gut bacteria	452:471	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	1	23	theme	microorganisms	193:206	arg1	microbiome					154:163	The intestinal microbiome	139:163	The intestinal microbiome	139:163	The intestinal microbiome is a community of anaerobic microorganisms whose activities significantly impact human health.					
37316088	1	23	theme	microorganisms	193:206	arg1	community					170:178	a community	168:178	a community of anaerobic microorganisms whose activities significantly impact human health	168:257	The intestinal microbiome is a community of anaerobic microorganisms whose activities significantly impact human health.					
37316088	8	24	theme	relative	1199:1206	arg1	abundance					1208:1216	the relative abundance	1195:1216	the relative abundance of these proteins	1195:1234	Interestingly, the relative abundance of these proteins remains largely unaffected in the presence of Bifidobacterium longum PT4.					
37316088	5	25	theme	unidirectional	764:777	arg1	assays					779:784	unidirectional assays	764:784	unidirectional assays	764:784	Results from unidirectional assays gave indications of possible cross-feeding between bacteria using xylan as a carbon source.					
37316088	4	26	dep	one	738:740	arg1	another					742:748	another	742:748	another	742:748	Different bacterial strains of Lactobacillus, Bifidobacterium, and Bacteroides were evaluated for their ability to consume xylan and interact with one another.					
37316088	4	27	theme	Bacteroides	658:668	arg1	strains					611:617	Different bacterial strains	591:617	Different bacterial strains of Lactobacillus, Bifidobacterium, and Bacteroides	591:668	Different bacterial strains of Lactobacillus, Bifidobacterium, and Bacteroides were evaluated for their ability to consume xylan and interact with one another.					
37316088	0	28	theme	Proteomic	0:8	arg1	analyses					10:17	Proteomic analyses	0:17	Proteomic analyses of Bacteroides ovatus and Bifidobacterium longum in xylan bidirectional culture	0:97	Proteomic analyses of Bacteroides ovatus and Bifidobacterium longum in xylan bidirectional culture shows sugar cross-feeding interactions.					
37316088	9	29	theme	enzymes	1382:1388	arg1	production					1368:1377	the production	1364:1377	the production of enzymes such as α-L-arabinosidase, L-arabinose isomerase, xylulose kinase, xylose isomerase, and sugar transporters	1364:1496	In the presence of B. ovatus, B. longum PT4 increased the production of enzymes such as α-L-arabinosidase, L-arabinose isomerase, xylulose kinase, xylose isomerase, and sugar transporters.					
37316088	7	30	theme	L-arabinose	1141:1151	arg1	isomerase					1153:1161	L-arabinose isomerase	1141:1161	L-arabinose isomerase	1141:1161	Proteomic analyses indicated that B. ovatus HM222 synthesizes enzymes facilitating xylan degradation, such as β-xylanase, arabinosidase, L-arabinose isomerase, and xylosidase.					
37316088	8	31	theme	proteins	1227:1234	arg1	abundance					1208:1216	the relative abundance	1195:1216	the relative abundance of these proteins	1195:1234	Interestingly, the relative abundance of these proteins remains largely unaffected in the presence of Bifidobacterium longum PT4.					
37316088	3	32	theme	other	559:563	arg1	bacteria					565:572	other bacteria	559:572	other bacteria	559:572	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	9	33	theme	sugar	1479:1483	arg1	transporters					1485:1496	sugar transporters	1479:1496	sugar transporters	1479:1496	In the presence of B. ovatus, B. longum PT4 increased the production of enzymes such as α-L-arabinosidase, L-arabinose isomerase, xylulose kinase, xylose isomerase, and sugar transporters.					
37316088	4	34	theme	Bifidobacterium	637:651	arg1	strains					611:617	Different bacterial strains	591:617	Different bacterial strains of Lactobacillus, Bifidobacterium, and Bacteroides	591:668	Different bacterial strains of Lactobacillus, Bifidobacterium, and Bacteroides were evaluated for their ability to consume xylan and interact with one another.					
37316088	9	35	dep	B.	1340:1341	arg1	longum					1343:1348	longum	1343:1348	longum	1343:1348	In the presence of B. ovatus, B. longum PT4 increased the production of enzymes such as α-L-arabinosidase, L-arabinose isomerase, xylulose kinase, xylose isomerase, and sugar transporters.					
37316088	10	36	theme	interaction	1541:1551	arg1	example					1521:1527	an example	1518:1527	an example of positive interaction between bacteria mediated by xylan consumption	1518:1598	These results show an example of positive interaction between bacteria mediated by xylan consumption.					
37316088	3	37	theme	certain	452:458	arg1	metabolites					542:552	releasing metabolites	532:552	releasing metabolites	532:552	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	3	37	theme	certain	452:458	arg1	fibers					520:525	fermenting dietary fibers	501:525	fermenting dietary fibers	501:525	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	3	37	theme	certain	452:458	arg1	degraders					490:498	primary degraders	482:498	primary degraders	482:498	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	3	37	theme	certain	452:458	arg1	bacteria					464:471	certain gut bacteria	452:471	certain gut bacteria	452:471	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	1	38	theme	human	246:250	arg1	health					252:257	human health	246:257	human health	246:257	The intestinal microbiome is a community of anaerobic microorganisms whose activities significantly impact human health.					
37316088	0	39	theme	ovatus	34:39	arg1	analyses					10:17	Proteomic analyses	0:17	Proteomic analyses of Bacteroides ovatus and Bifidobacterium longum in xylan bidirectional culture	0:97	Proteomic analyses of Bacteroides ovatus and Bifidobacterium longum in xylan bidirectional culture shows sugar cross-feeding interactions.					
37316088	6	40	theme	ovatus	990:995	arg1	presence					966:973	the presence	962:973	the presence of Bacteroides ovatus HM222	962:1001	Bidirectional assays showed that Bifidobacterium longum PT4 increased its growth in the presence of Bacteroides ovatus HM222.					
37316088	5	41	theme	possible	806:813	arg1	cross-feeding					815:827	possible cross-feeding	806:827	possible cross-feeding between bacteria using xylan as a carbon source	806:875	Results from unidirectional assays gave indications of possible cross-feeding between bacteria using xylan as a carbon source.					
37316088	11	42	theme	degraders	1754:1762	arg1	growth					1734:1739	the growth	1730:1739	the growth of secondary degraders such as B. longum	1730:1780	Bacteroides degraded this substrate to release xylooligosaccharides, or monosaccharides (xylose, arabinose), which might support the growth of secondary degraders such as B. longum.					
37316088	3	43	theme	gut	460:462	arg1	metabolites					542:552	releasing metabolites	532:552	releasing metabolites	532:552	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	3	43	theme	gut	460:462	arg1	fibers					520:525	fermenting dietary fibers	501:525	fermenting dietary fibers	501:525	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	3	43	theme	gut	460:462	arg1	degraders					490:498	primary degraders	482:498	primary degraders	482:498	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	3	43	theme	gut	460:462	arg1	bacteria					464:471	certain gut bacteria	452:471	certain gut bacteria	452:471	In this work, we evaluated how certain gut bacteria acted as primary degraders, fermenting dietary fibers, and releasing metabolites that other bacteria can further use.					
37316088	0	44	theme	longum	61:66	arg1	analyses					10:17	Proteomic analyses	0:17	Proteomic analyses of Bacteroides ovatus and Bifidobacterium longum in xylan bidirectional culture	0:97	Proteomic analyses of Bacteroides ovatus and Bifidobacterium longum in xylan bidirectional culture shows sugar cross-feeding interactions.					
37316088	6	45	theme	Bacteroides	978:988	arg1	ovatus					990:995	Bacteroides ovatus HM222	978:1001	Bacteroides ovatus HM222	978:1001	Bidirectional assays showed that Bifidobacterium longum PT4 increased its growth in the presence of Bacteroides ovatus HM222.					
37316088	6	46	theme	Bidirectional	878:890	arg1	assays					892:897	Bidirectional assays	878:897	Bidirectional assays	878:897	Bidirectional assays showed that Bifidobacterium longum PT4 increased its growth in the presence of Bacteroides ovatus HM222.					
37316088	2	47	theme	complex	352:358	arg1	xylan					343:347	xylan	343:347	xylan	343:347	Its composition can be modulated by consuming foods rich in dietary fiber, such as xylan, a complex polysaccharide that can be considered an emerging prebiotic.					
37316088	2	47	theme	complex	352:358	arg1	polysaccharide					360:373	a complex polysaccharide	350:373	a complex polysaccharide that can be considered an emerging prebiotic	350:418	Its composition can be modulated by consuming foods rich in dietary fiber, such as xylan, a complex polysaccharide that can be considered an emerging prebiotic.					
37316088	10	48	theme	positive	1532:1539	arg1	interaction					1541:1551	positive interaction	1532:1551	positive interaction between bacteria mediated by xylan consumption	1532:1598	These results show an example of positive interaction between bacteria mediated by xylan consumption.					
37316088	10	49	theme	xylan	1582:1586	arg1	consumption					1588:1598	xylan consumption	1582:1598	xylan consumption	1582:1598	These results show an example of positive interaction between bacteria mediated by xylan consumption.					
37316088	2	50	from	rich	312:315	arg1	fiber					328:332	dietary fiber	320:332	dietary fiber	320:332	Its composition can be modulated by consuming foods rich in dietary fiber, such as xylan, a complex polysaccharide that can be considered an emerging prebiotic.					
37316088	7	51	theme	xylan	1087:1091	arg1	degradation					1093:1103	xylan degradation	1087:1103	xylan degradation	1087:1103	Proteomic analyses indicated that B. ovatus HM222 synthesizes enzymes facilitating xylan degradation, such as β-xylanase, arabinosidase, L-arabinose isomerase, and xylosidase.					
37316088	1	52	theme	intestinal	143:152	arg1	microbiome					154:163	The intestinal microbiome	139:163	The intestinal microbiome	139:163	The intestinal microbiome is a community of anaerobic microorganisms whose activities significantly impact human health.					
37316088	1	52	theme	intestinal	143:152	arg1	community					170:178	a community	168:178	a community of anaerobic microorganisms whose activities significantly impact human health	168:257	The intestinal microbiome is a community of anaerobic microorganisms whose activities significantly impact human health.					
37316088	9	53	theme	xylulose	1440:1447	arg1	kinase					1449:1454	xylulose kinase	1440:1454	xylulose kinase	1440:1454	In the presence of B. ovatus, B. longum PT4 increased the production of enzymes such as α-L-arabinosidase, L-arabinose isomerase, xylulose kinase, xylose isomerase, and sugar transporters.					
37316088	7	54	theme	B.	1038:1039	arg1	HM222					1048:1052	B. ovatus HM222	1038:1052	B. ovatus HM222	1038:1052	Proteomic analyses indicated that B. ovatus HM222 synthesizes enzymes facilitating xylan degradation, such as β-xylanase, arabinosidase, L-arabinose isomerase, and xylosidase.					
37316088	2	55	dep	such	335:338	arg1	as					340:341	as	340:341	as	340:341	Its composition can be modulated by consuming foods rich in dietary fiber, such as xylan, a complex polysaccharide that can be considered an emerging prebiotic.					
37316088	8	56	theme	longum	1298:1303	arg1	PT4					1305:1307	Bifidobacterium longum PT4	1282:1307	Bifidobacterium longum PT4	1282:1307	Interestingly, the relative abundance of these proteins remains largely unaffected in the presence of Bifidobacterium longum PT4.					
37316088	0	57	theme	xylan	71:75	arg1	culture					91:97	xylan bidirectional culture	71:97	xylan bidirectional culture	71:97	Proteomic analyses of Bacteroides ovatus and Bifidobacterium longum in xylan bidirectional culture shows sugar cross-feeding interactions.					
37316088	5	58	from	assays	779:784	arg1	Results					751:757	Results	751:757	Results from unidirectional assays	751:784	Results from unidirectional assays gave indications of possible cross-feeding between bacteria using xylan as a carbon source.					
37316088	7	59	theme	ovatus	1041:1046	arg1	HM222					1048:1052	B. ovatus HM222	1038:1052	B. ovatus HM222	1038:1052	Proteomic analyses indicated that B. ovatus HM222 synthesizes enzymes facilitating xylan degradation, such as β-xylanase, arabinosidase, L-arabinose isomerase, and xylosidase.					
37316088	2	60	theme	emerging	401:408	arg1	prebiotic					410:418	an emerging prebiotic	398:418	an emerging prebiotic	398:418	Its composition can be modulated by consuming foods rich in dietary fiber, such as xylan, a complex polysaccharide that can be considered an emerging prebiotic.					
37316088	4	61	theme	Different	591:599	arg1	strains					611:617	Different bacterial strains	591:617	Different bacterial strains of Lactobacillus, Bifidobacterium, and Bacteroides	591:668	Different bacterial strains of Lactobacillus, Bifidobacterium, and Bacteroides were evaluated for their ability to consume xylan and interact with one another.					
37316088	5	62	theme	cross-feeding	815:827	arg1	indications					791:801	indications	791:801	indications of possible cross-feeding between bacteria using xylan as a carbon source	791:875	Results from unidirectional assays gave indications of possible cross-feeding between bacteria using xylan as a carbon source.					
37316088	9	63	theme	xylose	1457:1462	arg1	isomerase					1464:1472	xylose isomerase	1457:1472	xylose isomerase	1457:1472	In the presence of B. ovatus, B. longum PT4 increased the production of enzymes such as α-L-arabinosidase, L-arabinose isomerase, xylulose kinase, xylose isomerase, and sugar transporters.					
37316088	4	64	theme	Lactobacillus	622:634	arg1	strains					611:617	Different bacterial strains	591:617	Different bacterial strains of Lactobacillus, Bifidobacterium, and Bacteroides	591:668	Different bacterial strains of Lactobacillus, Bifidobacterium, and Bacteroides were evaluated for their ability to consume xylan and interact with one another.					
36986043	4	0	theme	prausnitzii	827:837	arg1	growth					839:844	F. prausnitzii growth	824:844	F. prausnitzii growth	824:844	Using a machine learning approach in combination with univariate analyses, we identified that sugar alcohols, carbocyclic sugar, and vitamins may contribute to F. prausnitzii growth.					
36986043	7	1	from	increase	1375:1382	arg1	prausnitzii					1390:1400	F. prausnitzii	1387:1400	F. prausnitzii	1387:1400	However, the fecal communities that showed an increase in F. prausnitzii on inulin also responded with at least 60% more F. prausnitzii on any of inositol containing media than control.					
36986043	6	2	with	combinations	1142:1153	arg1	B					1168:1168	vitamin B	1160:1168	vitamin B	1160:1168	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	6	3	theme	microbiota	1282:1291	arg1	community					1293:1301	the fecal microbiota community	1272:1301	the fecal microbiota community from four healthy donors	1272:1326	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	1	4	theme	healthy	349:355	arg1	habits					364:369	healthy eating habits	349:369	healthy eating habits	349:369	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	4	5	theme	F.	824:825	arg1	growth					839:844	F. prausnitzii growth	824:844	F. prausnitzii growth	824:844	Using a machine learning approach in combination with univariate analyses, we identified that sugar alcohols, carbocyclic sugar, and vitamins may contribute to F. prausnitzii growth.					
36986043	1	6	theme	Faecalibacterium	177:192	arg1	prausnitzii					194:204	Faecalibacterium prausnitzii	177:204	Faecalibacterium prausnitzii (F. prausnitzii)	177:221	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	1	6	theme	Faecalibacterium	177:192	arg1	prausnitzii					210:220	F. prausnitzii	207:220	F. prausnitzii	207:220	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	1	6	theme	Faecalibacterium	177:192	arg1	taxon					238:242	a bacterial taxon	226:242	a bacterial taxon in the human gut with anti-inflammatory properties	226:293	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	1	7	theme	eating	357:362	arg1	habits					364:369	healthy eating habits	349:369	healthy eating habits	349:369	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	3	8	theme	microbiome	526:535	arg1	data					537:540	dietary and microbiome data	514:540	dietary and microbiome data from the American Gut Project (AGP)	514:576	Here, we combined dietary and microbiome data from the American Gut Project (AGP) to identify nutrients that may be linked to the relative abundance of F. prausnitzii.					
36986043	1	9	theme	anti-inflammatory	266:282	arg1	properties					284:293	anti-inflammatory properties	266:293	anti-inflammatory properties	266:293	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	6	10	theme	healthy	1313:1319	arg1	donors					1321:1326	four healthy donors	1308:1326	four healthy donors	1308:1326	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	1	11	theme	habits	364:369	arg1	effects					338:344	the beneficial effects	323:344	the beneficial effects of healthy eating habits	323:369	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	7	12	theme	F.	1387:1388	arg1	prausnitzii					1390:1400	F. prausnitzii	1387:1400	F. prausnitzii	1387:1400	However, the fecal communities that showed an increase in F. prausnitzii on inulin also responded with at least 60% more F. prausnitzii on any of inositol containing media than control.					
36986043	5	13	theme	F.	916:917	arg1	strains					931:937	two F. prausnitzii strains	912:937	two F. prausnitzii strains	912:937	We next explored the effects of these nutrients on the growth of two F. prausnitzii strains in vitro and observed robust and strain-dependent growth patterns on sorbitol and inositol, respectively.					
36986043	8	14	theme	strain-level	1656:1667	arg1	variations					1677:1686	strain-level genetic variations	1656:1686	strain-level genetic variations	1656:1686	Future nutritional studies aiming to increase the relative abundance of F. prausnitzii should explore a personalized approach accounting for strain-level genetic variations and community-level microbiome composition.					
36986043	5	15	theme	nutrients	885:893	arg1	effects					868:874	the effects	864:874	the effects of these nutrients on the growth of two F. prausnitzii strains in vitro	864:946	We next explored the effects of these nutrients on the growth of two F. prausnitzii strains in vitro and observed robust and strain-dependent growth patterns on sorbitol and inositol, respectively.					
36986043	6	16	theme	F.	1219:1220	arg1	prausnitzii					1222:1232	F. prausnitzii	1219:1232	F. prausnitzii	1219:1232	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	3	17	theme	dietary	514:520	arg1	data					537:540	dietary and microbiome data	514:540	dietary and microbiome data from the American Gut Project (AGP)	514:576	Here, we combined dietary and microbiome data from the American Gut Project (AGP) to identify nutrients that may be linked to the relative abundance of F. prausnitzii.					
36986043	0	18	theme	Inositol	103:110	arg1	Substrate					133:141	a Strain-Specific Substrate	115:141	a Strain-Specific Substrate for Faecalibacterium prausnitzii	115:174	Application of Computational Data Modeling to a Large-Scale Population Cohort Assists the Discovery of Inositol as a Strain-Specific Substrate for Faecalibacterium prausnitzii.					
36986043	0	18	theme	Inositol	103:110	arg1	Discovery					90:98	the Discovery	86:98	the Discovery of Inositol	86:110	Application of Computational Data Modeling to a Large-Scale Population Cohort Assists the Discovery of Inositol as a Strain-Specific Substrate for Faecalibacterium prausnitzii.					
36986043	6	19	from	donors	1321:1326	arg1	community					1293:1301	the fecal microbiota community	1272:1301	the fecal microbiota community from four healthy donors	1272:1326	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	8	20	theme	relative	1565:1572	arg1	abundance					1574:1582	the relative abundance	1561:1582	the relative abundance of F. prausnitzii	1561:1600	Future nutritional studies aiming to increase the relative abundance of F. prausnitzii should explore a personalized approach accounting for strain-level genetic variations and community-level microbiome composition.					
36986043	5	21	theme	strains	931:937	arg1	growth					902:907	the growth	898:907	the growth of two F. prausnitzii strains in vitro	898:946	We next explored the effects of these nutrients on the growth of two F. prausnitzii strains in vitro and observed robust and strain-dependent growth patterns on sorbitol and inositol, respectively.					
36986043	6	22	dep	in	1089:1090	arg1	vitro					1092:1096	vitro	1092:1096	vitro	1092:1096	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	2	23	theme	F.	444:445	arg1	prausnitzii					447:457	F. prausnitzii	444:457	F. prausnitzii other than simple sugars and fibers	444:493	However, little is known about the nutrients that enhance the growth of F. prausnitzii other than simple sugars and fibers.					
36986043	8	24	theme	nutritional	1522:1532	arg1	studies					1534:1540	Future nutritional studies	1515:1540	Future nutritional studies aiming to increase the relative abundance of F. prausnitzii	1515:1600	Future nutritional studies aiming to increase the relative abundance of F. prausnitzii should explore a personalized approach accounting for strain-level genetic variations and community-level microbiome composition.					
36986043	3	25	theme	American	551:558	arg1	AGP					573:575	AGP	573:575	AGP	573:575	Here, we combined dietary and microbiome data from the American Gut Project (AGP) to identify nutrients that may be linked to the relative abundance of F. prausnitzii.					
36986043	3	25	theme	American	551:558	arg1	Project					564:570	the American Gut Project	547:570	the American Gut Project (AGP)	547:576	Here, we combined dietary and microbiome data from the American Gut Project (AGP) to identify nutrients that may be linked to the relative abundance of F. prausnitzii.					
36986043	8	26	theme	genetic	1669:1675	arg1	variations					1677:1686	strain-level genetic variations	1656:1686	strain-level genetic variations	1656:1686	Future nutritional studies aiming to increase the relative abundance of F. prausnitzii should explore a personalized approach accounting for strain-level genetic variations and community-level microbiome composition.					
36986043	1	27	theme	F.	207:208	arg1	prausnitzii					194:204	Faecalibacterium prausnitzii	177:204	Faecalibacterium prausnitzii (F. prausnitzii)	177:221	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	1	27	theme	F.	207:208	arg1	prausnitzii					210:220	F. prausnitzii	207:220	F. prausnitzii	207:220	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	3	28	theme	F.	648:649	arg1	prausnitzii					651:661	F. prausnitzii	648:661	F. prausnitzii	648:661	Here, we combined dietary and microbiome data from the American Gut Project (AGP) to identify nutrients that may be linked to the relative abundance of F. prausnitzii.					
36986043	6	29	theme	growth-promoting	1192:1207	arg1	effect					1209:1214	a significant growth-promoting effect	1178:1214	a significant growth-promoting effect	1178:1214	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	5	30	from	effects	868:874	arg1	growth					902:907	the growth	898:907	the growth of two F. prausnitzii strains in vitro	898:946	We next explored the effects of these nutrients on the growth of two F. prausnitzii strains in vitro and observed robust and strain-dependent growth patterns on sorbitol and inositol, respectively.					
36986043	3	31	theme	Gut	560:562	arg1	AGP					573:575	AGP	573:575	AGP	573:575	Here, we combined dietary and microbiome data from the American Gut Project (AGP) to identify nutrients that may be linked to the relative abundance of F. prausnitzii.					
36986043	3	31	theme	Gut	560:562	arg1	Project					564:570	the American Gut Project	547:570	the American Gut Project (AGP)	547:576	Here, we combined dietary and microbiome data from the American Gut Project (AGP) to identify nutrients that may be linked to the relative abundance of F. prausnitzii.					
36986043	3	32	from	Project	564:570	arg1	data					537:540	dietary and microbiome data	514:540	dietary and microbiome data from the American Gut Project (AGP)	514:576	Here, we combined dietary and microbiome data from the American Gut Project (AGP) to identify nutrients that may be linked to the relative abundance of F. prausnitzii.					
36986043	0	33	theme	Data	29:32	arg1	Modeling					34:41	Computational Data Modeling	15:41	Computational Data Modeling	15:41	Application of Computational Data Modeling to a Large-Scale Population Cohort Assists the Discovery of Inositol as a Strain-Specific Substrate for Faecalibacterium prausnitzii.					
36986043	0	34	theme	Strain-Specific	117:131	arg1	Substrate					133:141	a Strain-Specific Substrate	115:141	a Strain-Specific Substrate for Faecalibacterium prausnitzii	115:174	Application of Computational Data Modeling to a Large-Scale Population Cohort Assists the Discovery of Inositol as a Strain-Specific Substrate for Faecalibacterium prausnitzii.					
36986043	0	34	theme	Strain-Specific	117:131	arg1	Discovery					90:98	the Discovery	86:98	the Discovery of Inositol	86:110	Application of Computational Data Modeling to a Large-Scale Population Cohort Assists the Discovery of Inositol as a Strain-Specific Substrate for Faecalibacterium prausnitzii.					
36986043	4	35	theme	univariate	718:727	arg1	analyses					729:736	univariate analyses	718:736	univariate analyses	718:736	Using a machine learning approach in combination with univariate analyses, we identified that sugar alcohols, carbocyclic sugar, and vitamins may contribute to F. prausnitzii growth.					
36986043	6	36	theme	significant	1180:1190	arg1	effect					1209:1214	a significant growth-promoting effect	1178:1214	a significant growth-promoting effect	1178:1214	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	0	37	theme	Computational	15:27	arg1	Modeling					34:41	Computational Data Modeling	15:41	Computational Data Modeling	15:41	Application of Computational Data Modeling to a Large-Scale Population Cohort Assists the Discovery of Inositol as a Strain-Specific Substrate for Faecalibacterium prausnitzii.					
36986043	5	38	theme	prausnitzii	919:929	arg1	strains					931:937	two F. prausnitzii strains	912:937	two F. prausnitzii strains	912:937	We next explored the effects of these nutrients on the growth of two F. prausnitzii strains in vitro and observed robust and strain-dependent growth patterns on sorbitol and inositol, respectively.					
36986043	6	39	theme	in	1089:1090	arg1	fermentation					1098:1109	in vitro fermentation	1089:1109	in vitro fermentation	1089:1109	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	8	40	theme	community-level	1692:1706	arg1	composition					1719:1729	community-level microbiome composition	1692:1729	community-level microbiome composition	1692:1729	Future nutritional studies aiming to increase the relative abundance of F. prausnitzii should explore a personalized approach accounting for strain-level genetic variations and community-level microbiome composition.					
36986043	2	41	theme	simple	470:475	arg1	sugars					477:482	simple sugars	470:482	simple sugars	470:482	However, little is known about the nutrients that enhance the growth of F. prausnitzii other than simple sugars and fibers.					
36986043	8	42	theme	Future	1515:1520	arg1	studies					1534:1540	Future nutritional studies	1515:1540	Future nutritional studies aiming to increase the relative abundance of F. prausnitzii	1515:1600	Future nutritional studies aiming to increase the relative abundance of F. prausnitzii should explore a personalized approach accounting for strain-level genetic variations and community-level microbiome composition.					
36986043	6	43	theme	fecal	1276:1280	arg1	community					1293:1301	the fecal microbiota community	1272:1301	the fecal microbiota community from four healthy donors	1272:1326	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	5	44	theme	robust	961:966	arg1	patterns					996:1003	robust and strain-dependent growth patterns	961:1003	robust and strain-dependent growth patterns	961:1003	We next explored the effects of these nutrients on the growth of two F. prausnitzii strains in vitro and observed robust and strain-dependent growth patterns on sorbitol and inositol, respectively.					
36986043	7	45	theme	F.	1450:1451	arg1	prausnitzii					1453:1463	at least 60% more F. prausnitzii	1432:1463	at least 60% more F. prausnitzii	1432:1463	However, the fecal communities that showed an increase in F. prausnitzii on inulin also responded with at least 60% more F. prausnitzii on any of inositol containing media than control.					
36986043	0	46	theme	Modeling	34:41	arg1	Application					0:10	Application	0:10	Application of Computational Data Modeling to a Large-Scale Population Cohort	0:76	Application of Computational Data Modeling to a Large-Scale Population Cohort Assists the Discovery of Inositol as a Strain-Specific Substrate for Faecalibacterium prausnitzii.					
36986043	3	47	attach	linked	612:617	arg2	nutrients					590:598	nutrients	590:598	nutrients that may be linked to the relative abundance of F. prausnitzii	590:661	Here, we combined dietary and microbiome data from the American Gut Project (AGP) to identify nutrients that may be linked to the relative abundance of F. prausnitzii.					
36986043	3	47	attach	linked	612:617	arg1	abundance					635:643	the relative abundance	622:643	the relative abundance of F. prausnitzii	622:661	Here, we combined dietary and microbiome data from the American Gut Project (AGP) to identify nutrients that may be linked to the relative abundance of F. prausnitzii.					
36986043	4	48	theme	carbocyclic	774:784	arg1	sugar					786:790	carbocyclic sugar	774:790	carbocyclic sugar	774:790	Using a machine learning approach in combination with univariate analyses, we identified that sugar alcohols, carbocyclic sugar, and vitamins may contribute to F. prausnitzii growth.					
36986043	7	49	contain	containing	1484:1493	arg1	inositol					1475:1482	inositol	1475:1482	inositol containing media than control	1475:1512	However, the fecal communities that showed an increase in F. prausnitzii on inulin also responded with at least 60% more F. prausnitzii on any of inositol containing media than control.					
36986043	7	49	contain	containing	1484:1493	arg2	media					1495:1499	media	1495:1499	media than control	1495:1512	However, the fecal communities that showed an increase in F. prausnitzii on inulin also responded with at least 60% more F. prausnitzii on any of inositol containing media than control.					
36986043	1	50	theme	bacterial	228:236	arg1	taxon					238:242	a bacterial taxon	226:242	a bacterial taxon in the human gut with anti-inflammatory properties	226:293	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	1	50	theme	bacterial	228:236	arg1	prausnitzii					194:204	Faecalibacterium prausnitzii	177:204	Faecalibacterium prausnitzii (F. prausnitzii)	177:221	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	0	51	theme	Large-Scale	48:58	arg1	Cohort					71:76	a Large-Scale Population Cohort	46:76	a Large-Scale Population Cohort	46:76	Application of Computational Data Modeling to a Large-Scale Population Cohort Assists the Discovery of Inositol as a Strain-Specific Substrate for Faecalibacterium prausnitzii.					
36986043	8	52	theme	microbiome	1708:1717	arg1	composition					1719:1729	community-level microbiome composition	1692:1729	community-level microbiome composition	1692:1729	Future nutritional studies aiming to increase the relative abundance of F. prausnitzii should explore a personalized approach accounting for strain-level genetic variations and community-level microbiome composition.					
36986043	0	53	theme	Population	60:69	arg1	Cohort					71:76	a Large-Scale Population Cohort	46:76	a Large-Scale Population Cohort	46:76	Application of Computational Data Modeling to a Large-Scale Population Cohort Assists the Discovery of Inositol as a Strain-Specific Substrate for Faecalibacterium prausnitzii.					
36986043	5	54	theme	strain-dependent	972:987	arg1	patterns					996:1003	robust and strain-dependent growth patterns	961:1003	robust and strain-dependent growth patterns	961:1003	We next explored the effects of these nutrients on the growth of two F. prausnitzii strains in vitro and observed robust and strain-dependent growth patterns on sorbitol and inositol, respectively.					
36986043	6	55	theme	high	1249:1252	arg1	variability					1254:1264	high variability	1249:1264	high variability among the fecal microbiota community from four healthy donors	1249:1326	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	4	56	theme	machine	672:678	arg1	approach					689:696	a machine learning approach	670:696	a machine learning approach	670:696	Using a machine learning approach in combination with univariate analyses, we identified that sugar alcohols, carbocyclic sugar, and vitamins may contribute to F. prausnitzii growth.					
36986043	2	57	theme	prausnitzii	447:457	arg1	growth					434:439	the growth	430:439	the growth of F. prausnitzii other than simple sugars and fibers	430:493	However, little is known about the nutrients that enhance the growth of F. prausnitzii other than simple sugars and fibers.					
36986043	4	58	with	combination	701:711	arg1	analyses					729:736	univariate analyses	718:736	univariate analyses	718:736	Using a machine learning approach in combination with univariate analyses, we identified that sugar alcohols, carbocyclic sugar, and vitamins may contribute to F. prausnitzii growth.					
36986043	0	59	theme	Faecalibacterium	147:162	arg1	prausnitzii					164:174	Faecalibacterium prausnitzii	147:174	Faecalibacterium prausnitzii	147:174	Application of Computational Data Modeling to a Large-Scale Population Cohort Assists the Discovery of Inositol as a Strain-Specific Substrate for Faecalibacterium prausnitzii.					
36986043	7	60	theme	more	1445:1448	arg1	prausnitzii					1453:1463	at least 60% more F. prausnitzii	1432:1463	at least 60% more F. prausnitzii	1432:1463	However, the fecal communities that showed an increase in F. prausnitzii on inulin also responded with at least 60% more F. prausnitzii on any of inositol containing media than control.					
36986043	5	61	theme	growth	989:994	arg1	patterns					996:1003	robust and strain-dependent growth patterns	961:1003	robust and strain-dependent growth patterns	961:1003	We next explored the effects of these nutrients on the growth of two F. prausnitzii strains in vitro and observed robust and strain-dependent growth patterns on sorbitol and inositol, respectively.					
36986043	1	62	with	gut	257:259	arg1	properties					284:293	anti-inflammatory properties	266:293	anti-inflammatory properties	266:293	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	8	63	theme	prausnitzii	1590:1600	arg1	abundance					1574:1582	the relative abundance	1561:1582	the relative abundance of F. prausnitzii	1561:1600	Future nutritional studies aiming to increase the relative abundance of F. prausnitzii should explore a personalized approach accounting for strain-level genetic variations and community-level microbiome composition.					
36986043	1	64	theme	beneficial	327:336	arg1	effects					338:344	the beneficial effects	323:344	the beneficial effects of healthy eating habits	323:369	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	1	65	from	taxon	238:242	arg1	gut					257:259	the human gut	247:259	the human gut with anti-inflammatory properties	247:293	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36986043	6	66	theme	vitamin	1160:1166	arg1	B					1168:1168	vitamin B	1160:1168	vitamin B	1160:1168	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	4	67	theme	sugar	758:762	arg1	alcohols					764:771	sugar alcohols	758:771	sugar alcohols	758:771	Using a machine learning approach in combination with univariate analyses, we identified that sugar alcohols, carbocyclic sugar, and vitamins may contribute to F. prausnitzii growth.					
36986043	6	68	theme	complex	1065:1071	arg1	community					1073:1081	a complex community	1063:1081	a complex community using in vitro fermentation	1063:1109	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	7	69	theme	fecal	1342:1346	arg1	communities					1348:1358	the fecal communities	1338:1358	the fecal communities that showed an increase in F. prausnitzii on inulin	1338:1410	However, the fecal communities that showed an increase in F. prausnitzii on inulin also responded with at least 60% more F. prausnitzii on any of inositol containing media than control.					
36986043	4	70	theme	learning	680:687	arg1	approach					689:696	a machine learning approach	670:696	a machine learning approach	670:696	Using a machine learning approach in combination with univariate analyses, we identified that sugar alcohols, carbocyclic sugar, and vitamins may contribute to F. prausnitzii growth.					
36986043	3	71	theme	relative	626:633	arg1	abundance					635:643	the relative abundance	622:643	the relative abundance of F. prausnitzii	622:661	Here, we combined dietary and microbiome data from the American Gut Project (AGP) to identify nutrients that may be linked to the relative abundance of F. prausnitzii.					
36986043	3	72	theme	prausnitzii	651:661	arg1	abundance					635:643	the relative abundance	622:643	the relative abundance of F. prausnitzii	622:661	Here, we combined dietary and microbiome data from the American Gut Project (AGP) to identify nutrients that may be linked to the relative abundance of F. prausnitzii.					
36986043	8	73	theme	F.	1587:1588	arg1	prausnitzii					1590:1600	F. prausnitzii	1587:1600	F. prausnitzii	1587:1600	Future nutritional studies aiming to increase the relative abundance of F. prausnitzii should explore a personalized approach accounting for strain-level genetic variations and community-level microbiome composition.					
36986043	2	74	theme	other	459:463	arg1	prausnitzii					447:457	F. prausnitzii	444:457	F. prausnitzii other than simple sugars and fibers	444:493	However, little is known about the nutrients that enhance the growth of F. prausnitzii other than simple sugars and fibers.					
36986043	6	75	dep	community	1073:1081	arg1	context					1052:1058	context	1052:1058	context	1052:1058	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	6	75	dep	community	1073:1081	arg1	the					1048:1050	the	1048:1050	the	1048:1050	In the context of a complex community using in vitro fermentation, neither inositol alone nor in combinations with vitamin B exerted a significant growth-promoting effect on F. prausnitzii, partly due to high variability among the fecal microbiota community from four healthy donors.					
36986043	8	76	theme	personalized	1619:1630	arg1	approach					1632:1639	a personalized approach	1617:1639	a personalized approach accounting for strain-level genetic variations and community-level microbiome composition	1617:1729	Future nutritional studies aiming to increase the relative abundance of F. prausnitzii should explore a personalized approach accounting for strain-level genetic variations and community-level microbiome composition.					
36986043	1	77	theme	human	251:255	arg1	gut					257:259	the human gut	247:259	the human gut with anti-inflammatory properties	247:293	Faecalibacterium prausnitzii (F. prausnitzii) is a bacterial taxon in the human gut with anti-inflammatory properties, and this may contribute to the beneficial effects of healthy eating habits.					
36184160	0	0	theme	thermal	90:96	arg1	insulation					98:107	thermal insulation	90:107	thermal insulation	90:107	Biomimetic, hierarchical-ordered cellulose nanoclaw hybrid aerogel with high strength and thermal insulation.					
36184160	4	1	theme	excellent	850:858	arg1	properties					871:880	excellent mechanical properties	850:880	excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials	850:946	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	4	1	theme	excellent	850:858	arg1	16-58 MPa					883:891	16-58 MPa	883:891	16-58 MPa	883:891	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	4	2	theme	aerogel	720:726	arg1	layer					728:732	nanosized silica aerogel layer	703:732	nanosized silica aerogel layer	703:732	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	5	3	theme	surface	1099:1105	arg1	30-630 m2 g-1					1113:1125	30-630 m2 g-1	1113:1125	30-630 m2 g-1	1113:1125	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	5	3	theme	surface	1099:1105	arg1	area					1107:1110	high specific surface area	1085:1110	high specific surface area (30-630 m2 g-1)	1085:1126	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	4	4	theme	nanoclaws-based	637:651	arg1	aerogels					653:660	The novel cellulose nanoclaws-based aerogels	617:660	The novel cellulose nanoclaws-based aerogels extracted from corncob	617:683	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	6	5	theme	robust	1292:1297	arg1	materials					1299:1307	thermal-insulated and mechanically robust materials	1257:1307	thermal-insulated and mechanically robust materials	1257:1307	This hybrid strategy provides an avenue to produce thermal-insulated and mechanically robust materials.					
36184160	5	6	theme	high	1085:1088	arg1	30-630 m2 g-1					1113:1125	30-630 m2 g-1	1113:1125	30-630 m2 g-1	1113:1125	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	5	6	theme	high	1085:1088	arg1	area					1107:1110	high specific surface area	1085:1110	high specific surface area (30-630 m2 g-1)	1085:1126	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	4	7	theme	silica	713:718	arg1	layer					728:732	nanosized silica aerogel layer	703:732	nanosized silica aerogel layer	703:732	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	5	8	theme	outstanding	1177:1187	arg1	retardant					1195:1203	outstanding flame retardant	1177:1203	outstanding flame retardant	1177:1203	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	4	9	theme	cellulose	627:635	arg1	aerogels					653:660	The novel cellulose nanoclaws-based aerogels	617:660	The novel cellulose nanoclaws-based aerogels extracted from corncob	617:683	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	5	10	theme	thermal	1133:1139	arg1	conductivity					1141:1152	low thermal conductivity	1129:1152	low thermal conductivity (0.021 W m-1 K-1)	1129:1170	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	5	10	theme	thermal	1133:1139	arg1	0.021 W m-1 K-1					1155:1169	0.021 W m-1 K-1	1155:1169	0.021 W m-1 K-1	1155:1169	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	4	11	theme	ambient-drying	802:815	arg1	treatment					830:838	ambient-drying and hotpress treatment	802:838	ambient-drying and hotpress treatment	802:838	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	6	12	theme	mechanically	1279:1290	arg1	materials					1299:1307	thermal-insulated and mechanically robust materials	1257:1307	thermal-insulated and mechanically robust materials	1257:1307	This hybrid strategy provides an avenue to produce thermal-insulated and mechanically robust materials.					
36184160	4	13	theme	scalable	905:912	arg1	potential					914:922	scalable potential	905:922	excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials	850:946	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	5	14	theme	specific	1090:1097	arg1	30-630 m2 g-1					1113:1125	30-630 m2 g-1	1113:1125	30-630 m2 g-1	1113:1125	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	5	14	theme	specific	1090:1097	arg1	area					1107:1110	high specific surface area	1085:1110	high specific surface area (30-630 m2 g-1)	1085:1126	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	1	15	with	aerogels	168:175	arg1	properties					200:209	robust mechanical properties	182:209	robust mechanical properties	182:209	High-performance thermally insulating nanocellulose-based aerogels with robust mechanical properties is highly desirable for energy-saving and thermal protection applications.					
36184160	4	16	theme	intensified	754:764	arg1	network					788:794	an intensified H-bonding crosslinked network	751:794	an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment	751:838	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	2	17	theme	aerogels	353:360	arg1	applications					311:322	the large-scale applications	295:322	the large-scale applications of traditional nanocellulose aerogels	295:360	However, the large-scale applications of traditional nanocellulose aerogels are still limited by their brittleness nature and rigorous processes.					
36184160	4	18	theme	hotpress	821:828	arg1	treatment					830:838	ambient-drying and hotpress treatment	802:838	ambient-drying and hotpress treatment	802:838	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	3	19	theme	nanocellulose-based	520:538	arg1	aerogel					540:546	thermal insulated nanocellulose-based aerogel	502:546	thermal insulated nanocellulose-based aerogel	502:546	Herein, an effective biomimetic hybrid strategy for high strength and thermal insulated nanocellulose-based aerogel with hierarchical-ordered microstructure is designed and fabricated.					
36184160	2	20	theme	nanocellulose	339:351	arg1	aerogels					353:360	traditional nanocellulose aerogels	327:360	traditional nanocellulose aerogels	327:360	However, the large-scale applications of traditional nanocellulose aerogels are still limited by their brittleness nature and rigorous processes.					
36184160	3	21	theme	hybrid	464:469	arg1	strategy					471:478	an effective biomimetic hybrid strategy	440:478	an effective biomimetic hybrid strategy for high strength and thermal insulated nanocellulose-based aerogel with hierarchical-ordered microstructure	440:587	Herein, an effective biomimetic hybrid strategy for high strength and thermal insulated nanocellulose-based aerogel with hierarchical-ordered microstructure is designed and fabricated.					
36184160	6	22	theme	thermal-insulated	1257:1273	arg1	materials					1299:1307	thermal-insulated and mechanically robust materials	1257:1307	thermal-insulated and mechanically robust materials	1257:1307	This hybrid strategy provides an avenue to produce thermal-insulated and mechanically robust materials.					
36184160	2	23	theme	brittleness	389:399	arg1	nature					401:406	their brittleness nature	383:406	their brittleness nature	383:406	However, the large-scale applications of traditional nanocellulose aerogels are still limited by their brittleness nature and rigorous processes.					
36184160	3	24	theme	thermal	502:508	arg1	aerogel					540:546	thermal insulated nanocellulose-based aerogel	502:546	thermal insulated nanocellulose-based aerogel	502:546	Herein, an effective biomimetic hybrid strategy for high strength and thermal insulated nanocellulose-based aerogel with hierarchical-ordered microstructure is designed and fabricated.					
36184160	0	25	theme	Biomimetic	0:9	arg1	hybrid					52:57	Biomimetic, hierarchical-ordered cellulose nanoclaw hybrid	0:57	Biomimetic, hierarchical-ordered cellulose nanoclaw hybrid	0:57	Biomimetic, hierarchical-ordered cellulose nanoclaw hybrid aerogel with high strength and thermal insulation.					
36184160	1	26	theme	energy-saving	235:247	arg1	applications					272:283	energy-saving and thermal protection applications	235:283	energy-saving and thermal protection applications	235:283	High-performance thermally insulating nanocellulose-based aerogels with robust mechanical properties is highly desirable for energy-saving and thermal protection applications.					
36184160	0	27	theme	cellulose	33:41	arg1	hybrid					52:57	Biomimetic, hierarchical-ordered cellulose nanoclaw hybrid	0:57	Biomimetic, hierarchical-ordered cellulose nanoclaw hybrid	0:57	Biomimetic, hierarchical-ordered cellulose nanoclaw hybrid aerogel with high strength and thermal insulation.					
36184160	5	28	theme	synergistic	970:980	arg1	effect					982:987	the synergistic effect	966:987	the synergistic effect of the tailored hierarchical-ordered microstructure	966:1039	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	1	29	theme	High-performance	110:125	arg1	aerogels					168:175	High-performance thermally insulating nanocellulose-based aerogels	110:175	High-performance thermally insulating nanocellulose-based aerogels with robust mechanical properties	110:209	High-performance thermally insulating nanocellulose-based aerogels with robust mechanical properties is highly desirable for energy-saving and thermal protection applications.					
36184160	5	30	theme	low	1129:1131	arg1	conductivity					1141:1152	low thermal conductivity	1129:1152	low thermal conductivity (0.021 W m-1 K-1)	1129:1170	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	5	30	theme	low	1129:1131	arg1	0.021 W m-1 K-1					1155:1169	0.021 W m-1 K-1	1155:1169	0.021 W m-1 K-1	1155:1169	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	4	31	theme	H-bonding	766:774	arg1	network					788:794	an intensified H-bonding crosslinked network	751:794	an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment	751:838	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	3	32	theme	biomimetic	453:462	arg1	strategy					471:478	an effective biomimetic hybrid strategy	440:478	an effective biomimetic hybrid strategy for high strength and thermal insulated nanocellulose-based aerogel with hierarchical-ordered microstructure	440:587	Herein, an effective biomimetic hybrid strategy for high strength and thermal insulated nanocellulose-based aerogel with hierarchical-ordered microstructure is designed and fabricated.					
36184160	4	33	theme	nanosized	703:711	arg1	layer					728:732	nanosized silica aerogel layer	703:732	nanosized silica aerogel layer	703:732	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	4	34	theme	structure	928:936	arg1	materials					938:946	structure materials	928:946	structure materials	928:946	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	3	35	with	strategy	471:478	arg1	microstructure					574:587	hierarchical-ordered microstructure	553:587	hierarchical-ordered microstructure	553:587	Herein, an effective biomimetic hybrid strategy for high strength and thermal insulated nanocellulose-based aerogel with hierarchical-ordered microstructure is designed and fabricated.					
36184160	1	36	theme	thermal	253:259	arg1	applications					272:283	energy-saving and thermal protection applications	235:283	energy-saving and thermal protection applications	235:283	High-performance thermally insulating nanocellulose-based aerogels with robust mechanical properties is highly desirable for energy-saving and thermal protection applications.					
36184160	2	37	theme	rigorous	412:419	arg1	processes					421:429	rigorous processes	412:429	rigorous processes	412:429	However, the large-scale applications of traditional nanocellulose aerogels are still limited by their brittleness nature and rigorous processes.					
36184160	5	38	theme	hierarchical-ordered	1005:1024	arg1	microstructure					1026:1039	the tailored hierarchical-ordered microstructure	992:1039	the tailored hierarchical-ordered microstructure	992:1039	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	5	39	theme	flame	1189:1193	arg1	retardant					1195:1203	outstanding flame retardant	1177:1203	outstanding flame retardant	1177:1203	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	3	40	theme	insulated	510:518	arg1	aerogel					540:546	thermal insulated nanocellulose-based aerogel	502:546	thermal insulated nanocellulose-based aerogel	502:546	Herein, an effective biomimetic hybrid strategy for high strength and thermal insulated nanocellulose-based aerogel with hierarchical-ordered microstructure is designed and fabricated.					
36184160	1	41	theme	protection	261:270	arg1	applications					272:283	energy-saving and thermal protection applications	235:283	energy-saving and thermal protection applications	235:283	High-performance thermally insulating nanocellulose-based aerogels with robust mechanical properties is highly desirable for energy-saving and thermal protection applications.					
36184160	0	42	theme	nanoclaw	43:50	arg1	hybrid					52:57	Biomimetic, hierarchical-ordered cellulose nanoclaw hybrid	0:57	Biomimetic, hierarchical-ordered cellulose nanoclaw hybrid	0:57	Biomimetic, hierarchical-ordered cellulose nanoclaw hybrid aerogel with high strength and thermal insulation.					
36184160	5	43	theme	resulting	1046:1054	arg1	aerogels					1063:1070	the resulting hybrid aerogels	1042:1070	the resulting hybrid aerogels	1042:1070	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	3	44	theme	effective	443:451	arg1	strategy					471:478	an effective biomimetic hybrid strategy	440:478	an effective biomimetic hybrid strategy for high strength and thermal insulated nanocellulose-based aerogel with hierarchical-ordered microstructure	440:587	Herein, an effective biomimetic hybrid strategy for high strength and thermal insulated nanocellulose-based aerogel with hierarchical-ordered microstructure is designed and fabricated.					
36184160	5	45	theme	microstructure	1026:1039	arg1	effect					982:987	the synergistic effect	966:987	the synergistic effect of the tailored hierarchical-ordered microstructure	966:1039	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	3	46	theme	hierarchical-ordered	553:572	arg1	microstructure					574:587	hierarchical-ordered microstructure	553:587	hierarchical-ordered microstructure	553:587	Herein, an effective biomimetic hybrid strategy for high strength and thermal insulated nanocellulose-based aerogel with hierarchical-ordered microstructure is designed and fabricated.					
36184160	6	47	theme	hybrid	1211:1216	arg1	strategy					1218:1225	This hybrid strategy	1206:1225	This hybrid strategy	1206:1225	This hybrid strategy provides an avenue to produce thermal-insulated and mechanically robust materials.					
36184160	1	48	theme	insulating	137:146	arg1	aerogels					168:175	High-performance thermally insulating nanocellulose-based aerogels	110:175	High-performance thermally insulating nanocellulose-based aerogels with robust mechanical properties	110:209	High-performance thermally insulating nanocellulose-based aerogels with robust mechanical properties is highly desirable for energy-saving and thermal protection applications.					
36184160	3	49	theme	high	484:487	arg1	strength					489:496	high strength	484:496	high strength	484:496	Herein, an effective biomimetic hybrid strategy for high strength and thermal insulated nanocellulose-based aerogel with hierarchical-ordered microstructure is designed and fabricated.					
36184160	1	50	theme	nanocellulose-based	148:166	arg1	aerogels					168:175	High-performance thermally insulating nanocellulose-based aerogels	110:175	High-performance thermally insulating nanocellulose-based aerogels with robust mechanical properties	110:209	High-performance thermally insulating nanocellulose-based aerogels with robust mechanical properties is highly desirable for energy-saving and thermal protection applications.					
36184160	0	51	dep	Biomimetic	0:9	arg1	hierarchical-ordered					12:31	hierarchical-ordered	12:31	hierarchical-ordered	12:31	Biomimetic, hierarchical-ordered cellulose nanoclaw hybrid aerogel with high strength and thermal insulation.					
36184160	4	52	contain	have	746:749	arg1	aerogels					653:660	The novel cellulose nanoclaws-based aerogels	617:660	The novel cellulose nanoclaws-based aerogels extracted from corncob	617:683	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	4	52	contain	have	746:749	arg2	network					788:794	an intensified H-bonding crosslinked network	751:794	an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment	751:838	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	2	53	theme	traditional	327:337	arg1	aerogels					353:360	traditional nanocellulose aerogels	327:360	traditional nanocellulose aerogels	327:360	However, the large-scale applications of traditional nanocellulose aerogels are still limited by their brittleness nature and rigorous processes.					
36184160	5	54	theme	hybrid	1056:1061	arg1	aerogels					1063:1070	the resulting hybrid aerogels	1042:1070	the resulting hybrid aerogels	1042:1070	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	4	55	link	crosslinked	776:786	arg1	network					788:794	an intensified H-bonding crosslinked network	751:794	an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment	751:838	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	5	56	theme	tailored	996:1003	arg1	microstructure					1026:1039	the tailored hierarchical-ordered microstructure	992:1039	the tailored hierarchical-ordered microstructure	992:1039	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	4	57	theme	crosslinked	776:786	arg1	network					788:794	an intensified H-bonding crosslinked network	751:794	an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment	751:838	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	5	58	contain	possess	1077:1083	arg2	30-630 m2 g-1					1113:1125	30-630 m2 g-1	1113:1125	30-630 m2 g-1	1113:1125	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	5	58	contain	possess	1077:1083	arg1	aerogels					1063:1070	the resulting hybrid aerogels	1042:1070	the resulting hybrid aerogels	1042:1070	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	5	58	contain	possess	1077:1083	arg2	conductivity					1141:1152	low thermal conductivity	1129:1152	low thermal conductivity (0.021 W m-1 K-1)	1129:1170	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	5	58	contain	possess	1077:1083	arg2	area					1107:1110	high specific surface area	1085:1110	high specific surface area (30-630 m2 g-1)	1085:1126	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	5	58	contain	possess	1077:1083	arg2	0.021 W m-1 K-1					1155:1169	0.021 W m-1 K-1	1155:1169	0.021 W m-1 K-1	1155:1169	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	5	58	contain	possess	1077:1083	arg2	retardant					1195:1203	outstanding flame retardant	1177:1203	outstanding flame retardant	1177:1203	Benefitting from the synergistic effect of the tailored hierarchical-ordered microstructure, the resulting hybrid aerogels also possess high specific surface area (30-630 m2 g-1), low thermal conductivity (0.021 W m-1 K-1), and outstanding flame retardant.					
36184160	1	59	theme	robust	182:187	arg1	properties					200:209	robust mechanical properties	182:209	robust mechanical properties	182:209	High-performance thermally insulating nanocellulose-based aerogels with robust mechanical properties is highly desirable for energy-saving and thermal protection applications.					
36184160	0	60	theme	high	72:75	arg1	strength					77:84	high strength	72:84	high strength	72:84	Biomimetic, hierarchical-ordered cellulose nanoclaw hybrid aerogel with high strength and thermal insulation.					
36184160	4	61	theme	novel	621:625	arg1	aerogels					653:660	The novel cellulose nanoclaws-based aerogels	617:660	The novel cellulose nanoclaws-based aerogels extracted from corncob	617:683	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	2	62	theme	large-scale	299:309	arg1	applications					311:322	the large-scale applications	295:322	the large-scale applications of traditional nanocellulose aerogels	295:360	However, the large-scale applications of traditional nanocellulose aerogels are still limited by their brittleness nature and rigorous processes.					
36184160	1	63	theme	mechanical	189:198	arg1	properties					200:209	robust mechanical properties	182:209	robust mechanical properties	182:209	High-performance thermally insulating nanocellulose-based aerogels with robust mechanical properties is highly desirable for energy-saving and thermal protection applications.					
36184160	4	64	theme	mechanical	860:869	arg1	properties					871:880	excellent mechanical properties	850:880	excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials	850:946	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
36184160	4	64	theme	mechanical	860:869	arg1	16-58 MPa					883:891	16-58 MPa	883:891	16-58 MPa	883:891	The novel cellulose nanoclaws-based aerogels extracted from corncob are hybridized by nanosized silica aerogel layer in situ and have an intensified H-bonding crosslinked network after ambient-drying and hotpress treatment, endowing excellent mechanical properties (16-58 MPa) as well as scalable potential for structure materials.					
37390998	4	0	theme	slight	486:491	arg1	decrease					493:500	a slight decrease	484:500	a slight decrease in transparency	484:516	The composite film showed a slight decrease in transparency but a significant increase in UV protection properties.					
37390998	6	1	theme	substantial	718:728	arg1	activity					744:751	substantial antibacterial activity	718:751	substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes	718:817	The film also unveiled substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes, fully eliminating the growth of these bacteria within 6 h of exposure.					
37390998	0	2	theme	banana	88:93	arg1	peel					95:98	banana peel	88:98	banana peel for active packaging application	88:131	Preparation of chitosan/gelatin-based functional films integrated with carbon dots from banana peel for active packaging application.					
37390998	7	3	theme	minced	944:949	arg1	packaging					956:964	minced meat packaging	944:964	minced meat packaging	944:964	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	5	4	theme	99 	655:657	arg1	%					658:658	%	658:658	%	658:658	The fabricated film displayed strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential.					
37390998	2	5	theme	amine	328:332	arg1	groups					334:339	amine groups	328:339	amine groups	328:339	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37390998	0	6	theme	active	104:109	arg1	application					121:131	active packaging application	104:131	active packaging application	104:131	Preparation of chitosan/gelatin-based functional films integrated with carbon dots from banana peel for active packaging application.					
37390998	4	7	from	decrease	493:500	arg1	transparency					505:516	transparency	505:516	transparency	505:516	The composite film showed a slight decrease in transparency but a significant increase in UV protection properties.					
37390998	5	8	theme	strong	604:609	arg1	efficacy					623:630	strong antioxidant efficacy	604:630	strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential	604:692	The fabricated film displayed strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential.					
37390998	7	9	theme	chitosan/gelatin	895:910	arg1	film					912:915	The chitosan/gelatin film	891:915	The chitosan/gelatin film containing CD	891:929	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	5	10	theme	ABTS	660:663	arg1	potential					684:692	99 % ABTS radical scavenging potential	655:692	99 % ABTS radical scavenging potential	655:692	The fabricated film displayed strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential.					
37390998	5	11	theme	antioxidant	611:621	arg1	efficacy					623:630	strong antioxidant efficacy	604:630	strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential	604:692	The fabricated film displayed strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential.					
37390998	4	12	theme	composite	462:470	arg1	film					472:475	The composite film	458:475	The composite film	458:475	The composite film showed a slight decrease in transparency but a significant increase in UV protection properties.					
37390998	2	13	from	groups	317:322	arg1	surface					348:354	the surface	344:354	the surface	344:354	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37390998	2	14	theme	spherical	259:267	arg1	particles					269:277	spherical particles	259:277	spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface	259:354	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37390998	2	14	theme	spherical	259:267	arg1	CDs					250:252	The synthesized CDs	234:252	The synthesized CDs	234:252	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37390998	0	15	theme	packaging	111:119	arg1	application					121:131	active packaging application	104:131	active packaging application	104:131	Preparation of chitosan/gelatin-based functional films integrated with carbon dots from banana peel for active packaging application.					
37390998	5	16	theme	>74 	640:643	arg1	%					644:644	%	644:644	%	644:644	The fabricated film displayed strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential.					
37390998	2	17	from	groups	334:339	arg1	surface					348:354	the surface	344:354	the surface	344:354	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37390998	1	18	theme	banana	175:180	arg1	peels					182:186	banana peels	175:186	banana peels using a hydrothermal method (200 °C for 6 h)	175:231	Carbon dots (CDs) were manufactured with banana peels using a hydrothermal method (200 °C for 6 h).					
37390998	8	19	theme	application	1171:1181	arg1	probability					1156:1166	a high probability	1149:1166	a high probability of application	1149:1181	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	8	20	theme	functional	1129:1138	arg1	film					1140:1143	The CD-added chitosan/gelatin functional film	1099:1143	The CD-added chitosan/gelatin functional film	1099:1143	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	5	21	theme	%	644:644	arg1	DPPH					646:649	>74 % DPPH	640:649	>74 % DPPH	640:649	The fabricated film displayed strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential.					
37390998	2	22	theme	1-3 nm	294:299	arg1	size					286:289	a size	284:289	a size of 1-3 nm having carboxyl groups and amine groups on the surface	284:354	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37390998	8	23	theme	shelf	1238:1242	arg1	life					1244:1247	the shelf life	1234:1247	the shelf life of packaged meat	1234:1264	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	4	24	theme	UV	548:549	arg1	properties					562:571	UV protection properties	548:571	UV protection properties	548:571	The composite film showed a slight decrease in transparency but a significant increase in UV protection properties.					
37390998	5	25	theme	fabricated	578:587	arg1	film					589:592	The fabricated film	574:592	The fabricated film	574:592	The fabricated film displayed strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential.					
37390998	0	26	theme	functional	38:47	arg1	films					49:53	chitosan/gelatin-based functional films	15:53	chitosan/gelatin-based functional films	15:53	Preparation of chitosan/gelatin-based functional films integrated with carbon dots from banana peel for active packaging application.					
37390998	6	27	theme	Listeria	796:803	arg1	monocytogenes					805:817	Listeria monocytogenes	796:817	Listeria monocytogenes	796:817	The film also unveiled substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes, fully eliminating the growth of these bacteria within 6 h of exposure.					
37390998	6	27	theme	Listeria	796:803	arg1	bacteria					786:793	the foodborne pathogenic bacteria	761:793	the foodborne pathogenic bacteria	761:793	The film also unveiled substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes, fully eliminating the growth of these bacteria within 6 h of exposure.					
37390998	7	28	theme	meat	951:954	arg1	packaging					956:964	minced meat packaging	944:964	minced meat packaging	944:964	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	7	29	dep	growth	998:1003	arg1	CFU/g					1014:1018	< 1 Log CFU/g	1006:1018	< 1 Log CFU/g	1006:1018	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	0	30	theme	chitosan/gelatin-based	15:36	arg1	films					49:53	chitosan/gelatin-based functional films	15:53	chitosan/gelatin-based functional films	15:53	Preparation of chitosan/gelatin-based functional films integrated with carbon dots from banana peel for active packaging application.					
37390998	8	31	contain	has	1145:1147	arg2	probability					1156:1166	a high probability	1149:1166	a high probability of application	1149:1181	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	8	31	contain	has	1145:1147	arg1	film					1140:1143	The CD-added chitosan/gelatin functional film	1099:1143	The CD-added chitosan/gelatin functional film	1099:1143	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	0	32	from	peel	95:98	arg1	dots					78:81	carbon dots	71:81	carbon dots from banana peel for active packaging application	71:131	Preparation of chitosan/gelatin-based functional films integrated with carbon dots from banana peel for active packaging application.					
37390998	1	33	theme	hydrothermal	196:207	arg1	method					209:214	a hydrothermal method	194:214	a hydrothermal method (200 °C for 6 h)	194:231	Carbon dots (CDs) were manufactured with banana peels using a hydrothermal method (200 °C for 6 h).					
37390998	4	34	from	increase	536:543	arg1	properties					562:571	UV protection properties	548:571	UV protection properties	548:571	The composite film showed a slight decrease in transparency but a significant increase in UV protection properties.					
37390998	4	35	theme	protection	551:560	arg1	properties					562:571	UV protection properties	548:571	UV protection properties	548:571	The composite film showed a slight decrease in transparency but a significant increase in UV protection properties.					
37390998	6	36	theme	exposure	881:888	arg1	6 h					874:876	6 h	874:876	6 h of exposure	874:888	The film also unveiled substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes, fully eliminating the growth of these bacteria within 6 h of exposure.					
37390998	7	37	contain	containing	917:926	arg1	film					912:915	The chitosan/gelatin film	891:915	The chitosan/gelatin film containing CD	891:929	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	7	37	contain	containing	917:926	arg2	CD					928:929	CD	928:929	CD	928:929	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	8	38	theme	aesthetic	1286:1294	arg1	quality					1296:1302	its aesthetic quality	1282:1302	its aesthetic quality	1282:1302	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	3	39	theme	packaging	441:449	arg1	films					451:455	multifunctional packaging films	425:455	multifunctional packaging films	425:455	CDs have been impregnated into chitosan/gelatin films to synthesize multifunctional packaging films.					
37390998	0	40	theme	films	49:53	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitosan/gelatin-based functional films	0:53	Preparation of chitosan/gelatin-based functional films integrated with carbon dots from banana peel for active packaging application.					
37390998	3	41	theme	chitosan/gelatin	388:403	arg1	films					405:409	chitosan/gelatin films	388:409	chitosan/gelatin films	388:409	CDs have been impregnated into chitosan/gelatin films to synthesize multifunctional packaging films.					
37390998	8	42	theme	meat	1261:1264	arg1	life					1244:1247	the shelf life	1234:1247	the shelf life of packaged meat	1234:1264	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	8	43	theme	active	1186:1191	arg1	packaging					1198:1206	active food packaging	1186:1206	active food packaging	1186:1206	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	8	44	theme	high	1151:1154	arg1	probability					1156:1166	a high probability	1149:1166	a high probability of application	1149:1181	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	0	45	theme	carbon	71:76	arg1	dots					78:81	carbon dots	71:81	carbon dots from banana peel for active packaging application	71:131	Preparation of chitosan/gelatin-based functional films integrated with carbon dots from banana peel for active packaging application.					
37390998	7	46	from	20 °C	1092:1096	arg1	storage					1081:1087	storage	1081:1087	storage at 20 °C	1081:1096	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	7	46	from	20 °C	1092:1096	arg1	24 h					1073:1076	24 h	1073:1076	24 h of storage at 20 °C	1073:1096	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	5	47	theme	radical	665:671	arg1	potential					684:692	99 % ABTS radical scavenging potential	655:692	99 % ABTS radical scavenging potential	655:692	The fabricated film displayed strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential.					
37390998	7	48	from	24 h	1073:1076	arg1	20 °C					1092:1096	20 °C	1092:1096	20 °C	1092:1096	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	6	49	theme	pathogenic	775:784	arg1	monocytogenes					805:817	Listeria monocytogenes	796:817	Listeria monocytogenes	796:817	The film also unveiled substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes, fully eliminating the growth of these bacteria within 6 h of exposure.					
37390998	6	49	theme	pathogenic	775:784	arg1	bacteria					786:793	the foodborne pathogenic bacteria	761:793	the foodborne pathogenic bacteria	761:793	The film also unveiled substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes, fully eliminating the growth of these bacteria within 6 h of exposure.					
37390998	7	50	theme	meat	1051:1054	arg1	color					1056:1060	the meat color	1047:1060	the meat color	1047:1060	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	5	51	theme	scavenging	673:682	arg1	potential					684:692	99 % ABTS radical scavenging potential	655:692	99 % ABTS radical scavenging potential	655:692	The fabricated film displayed strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential.					
37390998	5	52	theme	%	658:658	arg1	potential					684:692	99 % ABTS radical scavenging potential	655:692	99 % ABTS radical scavenging potential	655:692	The fabricated film displayed strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential.					
37390998	7	53	theme	storage	1081:1087	arg1	24 h					1073:1076	24 h	1073:1076	24 h of storage at 20 °C	1073:1096	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	6	54	theme	foodborne	765:773	arg1	monocytogenes					805:817	Listeria monocytogenes	796:817	Listeria monocytogenes	796:817	The film also unveiled substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes, fully eliminating the growth of these bacteria within 6 h of exposure.					
37390998	6	54	theme	foodborne	765:773	arg1	bacteria					786:793	the foodborne pathogenic bacteria	761:793	the foodborne pathogenic bacteria	761:793	The film also unveiled substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes, fully eliminating the growth of these bacteria within 6 h of exposure.					
37390998	1	55	theme	Carbon	134:139	arg1	dots					141:144	Carbon dots	134:144	Carbon dots (CDs)	134:150	Carbon dots (CDs) were manufactured with banana peels using a hydrothermal method (200 °C for 6 h).					
37390998	1	55	theme	Carbon	134:139	arg1	CDs					147:149	CDs	147:149	CDs	147:149	Carbon dots (CDs) were manufactured with banana peels using a hydrothermal method (200 °C for 6 h).					
37390998	4	56	theme	significant	524:534	arg1	increase					536:543	a significant increase	522:543	a significant increase in UV protection properties	522:571	The composite film showed a slight decrease in transparency but a significant increase in UV protection properties.					
37390998	6	57	theme	bacteria	858:865	arg1	growth					842:847	the growth	838:847	the growth of these bacteria	838:865	The film also unveiled substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes, fully eliminating the growth of these bacteria within 6 h of exposure.					
37390998	1	58	dep	method	209:214	arg1	200 °C					217:222	200 °C	217:222	200 °C for 6 h	217:230	Carbon dots (CDs) were manufactured with banana peels using a hydrothermal method (200 °C for 6 h).					
37390998	7	59	used	used	935:938	arg2	film					912:915	The chitosan/gelatin film	891:915	The chitosan/gelatin film containing CD	891:929	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	2	60	theme	carboxyl	308:315	arg1	groups					317:322	carboxyl groups	308:322	carboxyl groups	308:322	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37390998	7	61	theme	bacterial	988:996	arg1	growth					998:1003	bacterial growth	988:1003	bacterial growth (< 1 Log CFU/g after 24 h)	988:1030	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	7	62	theme	Log	1010:1012	arg1	CFU/g					1014:1018	< 1 Log CFU/g	1006:1018	< 1 Log CFU/g	1006:1018	The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C.					
37390998	2	63	theme	synthesized	238:248	arg1	particles					269:277	spherical particles	259:277	spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface	259:354	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37390998	2	63	theme	synthesized	238:248	arg1	CDs					250:252	The synthesized CDs	234:252	The synthesized CDs	234:252	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37390998	8	64	theme	food	1193:1196	arg1	packaging					1198:1206	active food packaging	1186:1206	active food packaging	1186:1206	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	8	65	theme	chitosan/gelatin	1112:1127	arg1	film					1140:1143	The CD-added chitosan/gelatin functional film	1099:1143	The CD-added chitosan/gelatin functional film	1099:1143	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	8	66	theme	packaged	1252:1259	arg1	meat					1261:1264	packaged meat	1252:1264	packaged meat	1252:1264	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	3	67	theme	multifunctional	425:439	arg1	films					451:455	multifunctional packaging films	425:455	multifunctional packaging films	425:455	CDs have been impregnated into chitosan/gelatin films to synthesize multifunctional packaging films.					
37390998	8	68	theme	CD-added	1103:1110	arg1	film					1140:1143	The CD-added chitosan/gelatin functional film	1099:1143	The CD-added chitosan/gelatin functional film	1099:1143	The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.					
37390998	2	69	contain	having	301:306	arg2	groups					334:339	amine groups	328:339	amine groups	328:339	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37390998	2	69	contain	having	301:306	arg2	groups					317:322	carboxyl groups	308:322	carboxyl groups	308:322	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37390998	2	69	contain	having	301:306	arg1	1-3 nm					294:299	1-3 nm	294:299	1-3 nm having carboxyl groups and amine groups on the surface	294:354	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37390998	6	70	theme	antibacterial	730:742	arg1	activity					744:751	substantial antibacterial activity	718:751	substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes	718:817	The film also unveiled substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes, fully eliminating the growth of these bacteria within 6 h of exposure.					
37390998	2	71	with	particles	269:277	arg1	size					286:289	a size	284:289	a size of 1-3 nm having carboxyl groups and amine groups on the surface	284:354	The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface.					
37058960	5	0	theme	air	1357:1359	arg1	condition					1361:1369	air condition	1357:1369	air condition for 30 days	1357:1381	The hydrogel also displayed tissue-adhesiveness and long-lasting water-retaining capacity, which acted as bio-electronic skin to stably acquire the electrophysiological signals and external stimuli even after the hydrogel was exposing to air condition for 30 days.					
37058960	6	1	theme	skin	1447:1450	arg1	dressing					1452:1459	a smart skin dressing	1439:1459	a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy	1439:1545	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	6	1	theme	skin	1447:1450	arg1	hydrogel					1410:1417	the transparent hydrogel	1394:1417	the transparent hydrogel	1394:1417	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	0	2	theme	sustainable	117:127	arg1	bioelectronics					129:142	sustainable bioelectronics	117:142	sustainable bioelectronics	117:142	Hydrogen-bonding topological remodeling modulated ultra-fine bacterial cellulose nanofibril-reinforced hydrogels for sustainable bioelectronics.					
37058960	3	3	theme	original	809:816	arg1	nanofibrils					821:831	the original BC nanofibrils	805:831	the original BC nanofibrils	805:831	Attributing to the hydrogen-bonding structural transition, the ultra-fine nanofibrils were extracted from the original BC nanofibrils, which reduced the light scattering and endowed the hydrogel with high transparency.					
37058960	5	4	theme	bio-electronic	1225:1238	arg1	skin					1240:1243	bio-electronic skin	1225:1243	bio-electronic skin	1225:1243	The hydrogel also displayed tissue-adhesiveness and long-lasting water-retaining capacity, which acted as bio-electronic skin to stably acquire the electrophysiological signals and external stimuli even after the hydrogel was exposing to air condition for 30 days.					
37058960	4	5	from	increase	1064:1071	arg1	toughness					1095:1103	toughness	1095:1103	toughness	1095:1103	Meanwhile, the extracted nanofibrils were connected with gelatin and glycerol to establish an effective energy dissipation network, leading to an increase in stretchability and toughness of hydrogels.					
37058960	4	5	from	increase	1064:1071	arg1	stretchability					1076:1089	stretchability	1076:1089	stretchability	1076:1089	Meanwhile, the extracted nanofibrils were connected with gelatin and glycerol to establish an effective energy dissipation network, leading to an increase in stretchability and toughness of hydrogels.					
37058960	1	6	theme	BC	405:406	arg1	structure					392:400	the hydrogen-bonding topological structure	359:400	the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability	359:472	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	4	7	theme	effective	1012:1020	arg1	dissipation					1029:1039	an effective energy dissipation	1009:1039	an effective energy dissipation network	1009:1047	Meanwhile, the extracted nanofibrils were connected with gelatin and glycerol to establish an effective energy dissipation network, leading to an increase in stretchability and toughness of hydrogels.					
37058960	7	8	theme	low	1742:1744	arg1	cost					1746:1749	low cost	1742:1749	low cost	1742:1749	This work offers a strategy to regulate the hierarchical structure of natural materials for designing skin-like bioelectronics toward green, low cost, and sustainability.					
37058960	6	9	theme	therapy	1539:1545	arg1	identification					1473:1486	optical identification	1465:1486	optical identification of bacterial infection and on-demand antibacterial therapy	1465:1545	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	2	10	theme	BC	695:696	arg1	structure					682:690	the hydrogen-bonding topological structure	649:690	the hydrogen-bonding topological structure of BC	649:696	Herein, we report an ultra-fine nanofibril-reinforced composite hydrogel by utilizing gelatin and glycerol as hydrogen-bonding donor/acceptor to mediate the rearrangement of the hydrogen-bonding topological structure of BC.					
37058960	6	11	theme	antibacterial	1525:1537	arg1	therapy					1539:1545	on-demand antibacterial therapy	1515:1545	on-demand antibacterial therapy	1515:1545	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	3	12	theme	light	852:856	arg1	scattering					858:867	the light scattering	848:867	the light scattering	848:867	Attributing to the hydrogen-bonding structural transition, the ultra-fine nanofibrils were extracted from the original BC nanofibrils, which reduced the light scattering and endowed the hydrogel with high transparency.					
37058960	6	13	theme	optical	1465:1471	arg1	identification					1473:1486	optical identification	1465:1486	optical identification of bacterial infection and on-demand antibacterial therapy	1465:1545	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	2	14	theme	composite	529:537	arg1	hydrogel					539:546	an ultra-fine nanofibril-reinforced composite hydrogel	493:546	an ultra-fine nanofibril-reinforced composite hydrogel	493:546	Herein, we report an ultra-fine nanofibril-reinforced composite hydrogel by utilizing gelatin and glycerol as hydrogen-bonding donor/acceptor to mediate the rearrangement of the hydrogen-bonding topological structure of BC.					
37058960	5	15	theme	electrophysiological	1267:1286	arg1	signals					1288:1294	the electrophysiological signals	1263:1294	the electrophysiological signals	1263:1294	The hydrogel also displayed tissue-adhesiveness and long-lasting water-retaining capacity, which acted as bio-electronic skin to stably acquire the electrophysiological signals and external stimuli even after the hydrogel was exposing to air condition for 30 days.					
37058960	5	16	theme	external	1300:1307	arg1	stimuli					1309:1315	external stimuli	1300:1315	external stimuli	1300:1315	The hydrogel also displayed tissue-adhesiveness and long-lasting water-retaining capacity, which acted as bio-electronic skin to stably acquire the electrophysiological signals and external stimuli even after the hydrogel was exposing to air condition for 30 days.					
37058960	2	17	theme	ultra-fine	496:505	arg1	hydrogel					539:546	an ultra-fine nanofibril-reinforced composite hydrogel	493:546	an ultra-fine nanofibril-reinforced composite hydrogel	493:546	Herein, we report an ultra-fine nanofibril-reinforced composite hydrogel by utilizing gelatin and glycerol as hydrogen-bonding donor/acceptor to mediate the rearrangement of the hydrogen-bonding topological structure of BC.					
37058960	1	18	theme	attractive	216:225	arg1	block					236:240	an attractive building block	213:240	an attractive building block for the fabrication of sustainable bioelectronics	213:290	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	1	18	theme	attractive	216:225	arg1	cellulose					155:163	Bacterial cellulose	145:163	Bacterial cellulose (BC) with its inherent nanofibrils framework	145:208	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	0	19	theme	topological	17:27	arg1	remodeling					29:38	Hydrogen-bonding topological remodeling	0:38	Hydrogen-bonding topological remodeling	0:38	Hydrogen-bonding topological remodeling modulated ultra-fine bacterial cellulose nanofibril-reinforced hydrogels for sustainable bioelectronics.					
37058960	1	20	theme	optical	423:429	arg1	transparency					431:442	its optical transparency	419:442	its optical transparency	419:442	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	6	21	theme	red	1574:1576	arg1	phenol					1567:1572	phenol red	1567:1576	phenol red	1567:1576	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	4	22	theme	energy	1022:1027	arg1	dissipation					1029:1039	an effective energy dissipation	1009:1039	an effective energy dissipation network	1009:1047	Meanwhile, the extracted nanofibrils were connected with gelatin and glycerol to establish an effective energy dissipation network, leading to an increase in stretchability and toughness of hydrogels.					
37058960	1	23	theme	building	227:234	arg1	block					236:240	an attractive building block	213:240	an attractive building block for the fabrication of sustainable bioelectronics	213:290	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	1	23	theme	building	227:234	arg1	cellulose					155:163	Bacterial cellulose	145:163	Bacterial cellulose (BC) with its inherent nanofibrils framework	145:208	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	0	24	theme	Hydrogen-bonding	0:15	arg1	remodeling					29:38	Hydrogen-bonding topological remodeling	0:38	Hydrogen-bonding topological remodeling	0:38	Hydrogen-bonding topological remodeling modulated ultra-fine bacterial cellulose nanofibril-reinforced hydrogels for sustainable bioelectronics.					
37058960	1	25	theme	effective	318:326	arg1	strategy					338:345	an effective and green strategy	315:345	an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability	315:472	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	3	26	with	endowed	873:879	arg1	transparency					904:915	high transparency	899:915	high transparency	899:915	Attributing to the hydrogen-bonding structural transition, the ultra-fine nanofibrils were extracted from the original BC nanofibrils, which reduced the light scattering and endowed the hydrogel with high transparency.					
37058960	1	27	theme	Bacterial	145:153	arg1	BC					166:167	BC	166:167	BC	166:167	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	1	27	theme	Bacterial	145:153	arg1	block					236:240	an attractive building block	213:240	an attractive building block for the fabrication of sustainable bioelectronics	213:290	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	1	27	theme	Bacterial	145:153	arg1	cellulose					155:163	Bacterial cellulose	145:163	Bacterial cellulose (BC) with its inherent nanofibrils framework	145:208	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	6	28	theme	smart	1441:1445	arg1	dressing					1452:1459	a smart skin dressing	1439:1459	a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy	1439:1545	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	6	28	theme	smart	1441:1445	arg1	hydrogel					1410:1417	the transparent hydrogel	1394:1417	the transparent hydrogel	1394:1417	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	6	29	theme	on-demand	1515:1523	arg1	therapy					1539:1545	on-demand antibacterial therapy	1515:1545	on-demand antibacterial therapy	1515:1545	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	1	30	theme	green	332:336	arg1	strategy					338:345	an effective and green strategy	315:345	an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability	315:472	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	6	31	theme	infection	1501:1509	arg1	identification					1473:1486	optical identification	1465:1486	optical identification of bacterial infection and on-demand antibacterial therapy	1465:1545	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	3	32	theme	ultra-fine	762:771	arg1	nanofibrils					773:783	the ultra-fine nanofibrils	758:783	the ultra-fine nanofibrils	758:783	Attributing to the hydrogen-bonding structural transition, the ultra-fine nanofibrils were extracted from the original BC nanofibrils, which reduced the light scattering and endowed the hydrogel with high transparency.					
37058960	0	33	theme	bacterial	61:69	arg1	hydrogels					103:111	ultra-fine bacterial cellulose nanofibril-reinforced hydrogels	50:111	ultra-fine bacterial cellulose nanofibril-reinforced hydrogels for sustainable bioelectronics	50:142	Hydrogen-bonding topological remodeling modulated ultra-fine bacterial cellulose nanofibril-reinforced hydrogels for sustainable bioelectronics.					
37058960	5	34	theme	tissue-adhesiveness	1147:1165	arg1	capacity					1200:1207	tissue-adhesiveness and long-lasting water-retaining capacity	1147:1207	tissue-adhesiveness and long-lasting water-retaining capacity	1147:1207	The hydrogel also displayed tissue-adhesiveness and long-lasting water-retaining capacity, which acted as bio-electronic skin to stably acquire the electrophysiological signals and external stimuli even after the hydrogel was exposing to air condition for 30 days.					
37058960	6	35	theme	bacterial	1491:1499	arg1	infection					1501:1509	bacterial infection	1491:1509	bacterial infection	1491:1509	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	6	36	theme	transparent	1398:1408	arg1	hydrogel					1410:1417	the transparent hydrogel	1394:1417	the transparent hydrogel	1394:1417	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	6	36	theme	transparent	1398:1408	arg1	dressing					1452:1459	a smart skin dressing	1439:1459	a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy	1439:1545	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	7	37	theme	natural	1671:1677	arg1	materials					1679:1687	natural materials	1671:1687	natural materials	1671:1687	This work offers a strategy to regulate the hierarchical structure of natural materials for designing skin-like bioelectronics toward green, low cost, and sustainability.					
37058960	0	38	theme	ultra-fine	50:59	arg1	hydrogels					103:111	ultra-fine bacterial cellulose nanofibril-reinforced hydrogels	50:111	ultra-fine bacterial cellulose nanofibril-reinforced hydrogels for sustainable bioelectronics	50:142	Hydrogen-bonding topological remodeling modulated ultra-fine bacterial cellulose nanofibril-reinforced hydrogels for sustainable bioelectronics.					
37058960	3	39	theme	high	899:902	arg1	transparency					904:915	high transparency	899:915	high transparency	899:915	Attributing to the hydrogen-bonding structural transition, the ultra-fine nanofibrils were extracted from the original BC nanofibrils, which reduced the light scattering and endowed the hydrogel with high transparency.					
37058960	1	40	theme	mechanical	448:457	arg1	stretchability					459:472	mechanical stretchability	448:472	mechanical stretchability	448:472	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	4	41	theme	extracted	933:941	arg1	nanofibrils					943:953	the extracted nanofibrils	929:953	the extracted nanofibrils	929:953	Meanwhile, the extracted nanofibrils were connected with gelatin and glycerol to establish an effective energy dissipation network, leading to an increase in stretchability and toughness of hydrogels.					
37058960	4	41	theme	extracted	933:941	arg1	Meanwhile					918:926	Meanwhile	918:926	Meanwhile	918:926	Meanwhile, the extracted nanofibrils were connected with gelatin and glycerol to establish an effective energy dissipation network, leading to an increase in stretchability and toughness of hydrogels.					
37058960	2	42	theme	hydrogen-bonding	585:600	arg1	donor/acceptor					602:615	hydrogen-bonding donor/acceptor	585:615	hydrogen-bonding donor/acceptor to mediate the rearrangement of the hydrogen-bonding topological structure of BC	585:696	Herein, we report an ultra-fine nanofibril-reinforced composite hydrogel by utilizing gelatin and glycerol as hydrogen-bonding donor/acceptor to mediate the rearrangement of the hydrogen-bonding topological structure of BC.					
37058960	0	43	theme	nanofibril-reinforced	81:101	arg1	hydrogels					103:111	ultra-fine bacterial cellulose nanofibril-reinforced hydrogels	50:111	ultra-fine bacterial cellulose nanofibril-reinforced hydrogels for sustainable bioelectronics	50:142	Hydrogen-bonding topological remodeling modulated ultra-fine bacterial cellulose nanofibril-reinforced hydrogels for sustainable bioelectronics.					
37058960	4	44	theme	dissipation	1029:1039	arg1	network					1041:1047	an effective energy dissipation network	1009:1047	an effective energy dissipation network	1009:1047	Meanwhile, the extracted nanofibrils were connected with gelatin and glycerol to establish an effective energy dissipation network, leading to an increase in stretchability and toughness of hydrogels.					
37058960	5	45	theme	water-retaining	1184:1198	arg1	capacity					1200:1207	tissue-adhesiveness and long-lasting water-retaining capacity	1147:1207	tissue-adhesiveness and long-lasting water-retaining capacity	1147:1207	The hydrogel also displayed tissue-adhesiveness and long-lasting water-retaining capacity, which acted as bio-electronic skin to stably acquire the electrophysiological signals and external stimuli even after the hydrogel was exposing to air condition for 30 days.					
37058960	0	46	theme	cellulose	71:79	arg1	hydrogels					103:111	ultra-fine bacterial cellulose nanofibril-reinforced hydrogels	50:111	ultra-fine bacterial cellulose nanofibril-reinforced hydrogels for sustainable bioelectronics	50:142	Hydrogen-bonding topological remodeling modulated ultra-fine bacterial cellulose nanofibril-reinforced hydrogels for sustainable bioelectronics.					
37058960	6	47	theme	indocyanine	1582:1592	arg1	green					1594:1598	indocyanine green	1582:1598	indocyanine green	1582:1598	Moreover, the transparent hydrogel could also serve as a smart skin dressing for optical identification of bacterial infection and on-demand antibacterial therapy after combined with phenol red and indocyanine green.					
37058960	2	48	theme	structure	682:690	arg1	rearrangement					632:644	the rearrangement	628:644	the rearrangement of the hydrogen-bonding topological structure of BC	628:696	Herein, we report an ultra-fine nanofibril-reinforced composite hydrogel by utilizing gelatin and glycerol as hydrogen-bonding donor/acceptor to mediate the rearrangement of the hydrogen-bonding topological structure of BC.					
37058960	1	49	theme	hydrogen-bonding	363:378	arg1	structure					392:400	the hydrogen-bonding topological structure	359:400	the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability	359:472	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	2	50	theme	topological	670:680	arg1	structure					682:690	the hydrogen-bonding topological structure	649:690	the hydrogen-bonding topological structure of BC	649:696	Herein, we report an ultra-fine nanofibril-reinforced composite hydrogel by utilizing gelatin and glycerol as hydrogen-bonding donor/acceptor to mediate the rearrangement of the hydrogen-bonding topological structure of BC.					
37058960	7	51	theme	skin-like	1703:1711	arg1	bioelectronics					1713:1726	skin-like bioelectronics	1703:1726	skin-like bioelectronics	1703:1726	This work offers a strategy to regulate the hierarchical structure of natural materials for designing skin-like bioelectronics toward green, low cost, and sustainability.					
37058960	3	52	theme	structural	735:744	arg1	transition					746:755	the hydrogen-bonding structural transition	714:755	the hydrogen-bonding structural transition	714:755	Attributing to the hydrogen-bonding structural transition, the ultra-fine nanofibrils were extracted from the original BC nanofibrils, which reduced the light scattering and endowed the hydrogel with high transparency.					
37058960	5	53	theme	long-lasting	1171:1182	arg1	capacity					1200:1207	tissue-adhesiveness and long-lasting water-retaining capacity	1147:1207	tissue-adhesiveness and long-lasting water-retaining capacity	1147:1207	The hydrogel also displayed tissue-adhesiveness and long-lasting water-retaining capacity, which acted as bio-electronic skin to stably acquire the electrophysiological signals and external stimuli even after the hydrogel was exposing to air condition for 30 days.					
37058960	1	54	theme	sustainable	265:275	arg1	bioelectronics					277:290	sustainable bioelectronics	265:290	sustainable bioelectronics	265:290	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	2	55	theme	hydrogen-bonding	653:668	arg1	structure					682:690	the hydrogen-bonding topological structure	649:690	the hydrogen-bonding topological structure of BC	649:696	Herein, we report an ultra-fine nanofibril-reinforced composite hydrogel by utilizing gelatin and glycerol as hydrogen-bonding donor/acceptor to mediate the rearrangement of the hydrogen-bonding topological structure of BC.					
37058960	7	56	theme	materials	1679:1687	arg1	structure					1658:1666	the hierarchical structure	1641:1666	the hierarchical structure of natural materials for designing skin-like bioelectronics toward green, low cost, and sustainability	1641:1769	This work offers a strategy to regulate the hierarchical structure of natural materials for designing skin-like bioelectronics toward green, low cost, and sustainability.					
37058960	1	57	theme	nanofibrils	188:198	arg1	framework					200:208	its inherent nanofibrils framework	175:208	its inherent nanofibrils framework	175:208	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	1	58	theme	bioelectronics	277:290	arg1	fabrication					250:260	the fabrication	246:260	the fabrication of sustainable bioelectronics	246:290	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	4	59	theme	hydrogels	1108:1116	arg1	toughness					1095:1103	toughness	1095:1103	toughness	1095:1103	Meanwhile, the extracted nanofibrils were connected with gelatin and glycerol to establish an effective energy dissipation network, leading to an increase in stretchability and toughness of hydrogels.					
37058960	4	59	theme	hydrogels	1108:1116	arg1	stretchability					1076:1089	stretchability	1076:1089	stretchability	1076:1089	Meanwhile, the extracted nanofibrils were connected with gelatin and glycerol to establish an effective energy dissipation network, leading to an increase in stretchability and toughness of hydrogels.					
37058960	3	60	theme	BC	818:819	arg1	nanofibrils					821:831	the original BC nanofibrils	805:831	the original BC nanofibrils	805:831	Attributing to the hydrogen-bonding structural transition, the ultra-fine nanofibrils were extracted from the original BC nanofibrils, which reduced the light scattering and endowed the hydrogel with high transparency.					
37058960	1	61	theme	inherent	179:186	arg1	framework					200:208	its inherent nanofibrils framework	175:208	its inherent nanofibrils framework	175:208	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	7	62	theme	hierarchical	1645:1656	arg1	structure					1658:1666	the hierarchical structure	1641:1666	the hierarchical structure of natural materials for designing skin-like bioelectronics toward green, low cost, and sustainability	1641:1769	This work offers a strategy to regulate the hierarchical structure of natural materials for designing skin-like bioelectronics toward green, low cost, and sustainability.					
37058960	2	63	theme	nanofibril-reinforced	507:527	arg1	hydrogel					539:546	an ultra-fine nanofibril-reinforced composite hydrogel	493:546	an ultra-fine nanofibril-reinforced composite hydrogel	493:546	Herein, we report an ultra-fine nanofibril-reinforced composite hydrogel by utilizing gelatin and glycerol as hydrogen-bonding donor/acceptor to mediate the rearrangement of the hydrogen-bonding topological structure of BC.					
37058960	1	64	theme	topological	380:390	arg1	structure					392:400	the hydrogen-bonding topological structure	359:400	the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability	359:472	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
37058960	3	65	theme	hydrogen-bonding	718:733	arg1	transition					746:755	the hydrogen-bonding structural transition	714:755	the hydrogen-bonding structural transition	714:755	Attributing to the hydrogen-bonding structural transition, the ultra-fine nanofibrils were extracted from the original BC nanofibrils, which reduced the light scattering and endowed the hydrogel with high transparency.					
37058960	1	66	with	cellulose	155:163	arg1	framework					200:208	its inherent nanofibrils framework	175:208	its inherent nanofibrils framework	175:208	Bacterial cellulose (BC) with its inherent nanofibrils framework is an attractive building block for the fabrication of sustainable bioelectronics, but there still lacks an effective and green strategy to regulate the hydrogen-bonding topological structure of BC to improve its optical transparency and mechanical stretchability.					
34883477	5	0	dep	architecture.In	1163:1177	arg1	demonstrated					1201:1212	demonstrated	1201:1212	demonstrated	1201:1212	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	8	1	theme	off-the-shelf	1923:1935	arg1	strategies					1938:1947	putative 'single-stage' or 'off-the-shelf' strategies	1895:1947	putative 'single-stage' or 'off-the-shelf' strategies for AC regeneration	1895:1967	Altogether, these findings demonstrate the potential of such biomimetic scaffolds as putative 'single-stage' or 'off-the-shelf' strategies for AC regeneration.					
34883477	2	2	used	used	441:444	arg2	scaffolds					417:425	articular cartilage (AC) mimetic scaffolds	384:425	articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration	384:495	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	2	2	used	used	441:444	arg2	implants					465:472	'off-the-shelf' implants	449:472	'off-the-shelf' implants for joint regeneration	449:495	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	3	3	theme	sulfated	530:537	arg1	glycosaminoglycan					539:555	a sulfated glycosaminoglycan	528:555	a sulfated glycosaminoglycan (sGAG)	528:562	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	3	3	theme	sulfated	530:537	arg1	sGAG					558:561	sGAG	558:561	sGAG	558:561	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	7	4	theme	dynamic	1786:1792	arg1	system					1802:1807	a dynamic culture system	1784:1807	a dynamic culture system	1784:1807	The ability of this composite scaffold to support chondrogenesis was then confirmed within a dynamic culture system.					
34883477	5	5	theme	MSCs	1287:1290	arg1	chondrogenesis					1269:1282	chondrogenesis	1269:1282	chondrogenesis of MSCs	1269:1290	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	6	theme	stem	976:979	arg1	MSC					987:989	MSC	987:989	MSC	987:989	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	6	theme	stem	976:979	arg1	cell					981:984	mesenchymal stem cell	964:984	mesenchymal stem cell (MSC) infiltration into the scaffold	964:1021	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	7	theme	cell	981:984	arg1	infiltration					992:1003	mesenchymal stem cell (MSC) infiltration	964:1003	mesenchymal stem cell (MSC) infiltration into the scaffold	964:1021	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	8	theme	tissue	1132:1137	arg1	development					1107:1117	the development	1103:1117	the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen	1103:1401	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	9	dep	enhance	1261:1267	arg1	both					1256:1259	both	1256:1259	both	1256:1259	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	10	theme	matrix	1084:1089	arg1	orientation					1041:1051	the orientation	1037:1051	the orientation of the deposited extracellular matrix	1037:1089	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	11	from	rich	1369:1372	arg1	sGAG					1377:1380	sGAG	1377:1380	sGAG	1377:1380	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	11	from	rich	1369:1372	arg1	collagen					1394:1401	type II collagen	1386:1401	type II collagen	1386:1401	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	4	12	theme	scaffold	818:825	arg1	architecture					827:838	scaffold architecture	818:838	scaffold architecture	818:838	Covalent crosslinking dramatically improved the elasticity of the alginate/alginate sulfate scaffolds, while scaffold architecture could be tailored using a directional freezing technique.					
34883477	4	13	theme	scaffolds	801:809	arg1	elasticity					757:766	the elasticity	753:766	the elasticity of the alginate/alginate sulfate scaffolds	753:809	Covalent crosslinking dramatically improved the elasticity of the alginate/alginate sulfate scaffolds, while scaffold architecture could be tailored using a directional freezing technique.					
34883477	5	14	from	sGAG	1377:1380	arg1	rich					1369:1372	rich	1369:1372	rich	1369:1372	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	4	15	theme	sulfate	793:799	arg1	scaffolds					801:809	the alginate/alginate sulfate scaffolds	771:809	the alginate/alginate sulfate scaffolds	771:809	Covalent crosslinking dramatically improved the elasticity of the alginate/alginate sulfate scaffolds, while scaffold architecture could be tailored using a directional freezing technique.					
34883477	5	16	theme	zonal	1157:1161	arg1	architecture.In					1163:1177	a biomimetic zonal architecture.In	1144:1177	a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen	1144:1401	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	8	17	theme	putative	1895:1902	arg1	strategies					1938:1947	putative 'single-stage' or 'off-the-shelf' strategies	1895:1947	putative 'single-stage' or 'off-the-shelf' strategies for AC regeneration	1895:1967	Altogether, these findings demonstrate the potential of such biomimetic scaffolds as putative 'single-stage' or 'off-the-shelf' strategies for AC regeneration.					
34883477	2	18	theme	manufacturing	325:337	arg1	strategies					362:371	additive manufacturing and biomaterial design strategies	316:371	additive manufacturing and biomaterial design strategies	316:371	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	5	19	theme	type	1386:1389	arg1	collagen					1394:1401	type II collagen	1386:1401	type II collagen	1386:1401	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	8	20	theme	AC	1953:1954	arg1	regeneration					1956:1967	AC regeneration	1953:1967	AC regeneration	1953:1967	Altogether, these findings demonstrate the potential of such biomimetic scaffolds as putative 'single-stage' or 'off-the-shelf' strategies for AC regeneration.					
34883477	3	21	used	used	575:578	arg2	sGAG					558:561	sGAG	558:561	sGAG	558:561	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	3	21	used	used	575:578	arg2	glycosaminoglycan					539:555	a sulfated glycosaminoglycan	528:555	a sulfated glycosaminoglycan (sGAG)	528:562	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	2	22	theme	additive	316:323	arg1	strategies					362:371	additive manufacturing and biomaterial design strategies	316:371	additive manufacturing and biomaterial design strategies	316:371	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	5	23	theme	biomimetic	1146:1155	arg1	architecture.In					1163:1177	a biomimetic zonal architecture.In	1144:1177	a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen	1144:1401	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	6	24	theme	printed	1452:1458	arg1	lactide-co-ϵ-caprolactone					1465:1489	lactide-co-ϵ-caprolactone	1465:1489	lactide-co-ϵ-caprolactone	1465:1489	The scaffolds were further reinforced with a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework, leading to composite implants that were more elastic than those reinforced with polycaprolactone, and which better mimicked the bulk mechanical properties of native cartilage tissue.					
34883477	6	24	theme	printed	1452:1458	arg1	PLCL					1493:1496	PLCL	1493:1496	PLCL	1493:1496	The scaffolds were further reinforced with a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework, leading to composite implants that were more elastic than those reinforced with polycaprolactone, and which better mimicked the bulk mechanical properties of native cartilage tissue.					
34883477	6	24	theme	printed	1452:1458	arg1	poly					1460:1463	printed poly	1452:1463	a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework	1447:1507	The scaffolds were further reinforced with a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework, leading to composite implants that were more elastic than those reinforced with polycaprolactone, and which better mimicked the bulk mechanical properties of native cartilage tissue.					
34883477	6	25	theme	tissue	1685:1690	arg1	properties					1654:1663	the bulk mechanical properties	1634:1663	the bulk mechanical properties of native cartilage tissue	1634:1690	The scaffolds were further reinforced with a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework, leading to composite implants that were more elastic than those reinforced with polycaprolactone, and which better mimicked the bulk mechanical properties of native cartilage tissue.					
34883477	2	26	theme	joint	478:482	arg1	regeneration					484:495	joint regeneration	478:495	joint regeneration	478:495	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	1	27	theme	tissue	208:213	arg1	engineering					215:225	tissue engineering	208:225	tissue engineering	208:225	Biomimetic scaffolds that provide a tissue-specific environment to cells are particularly promising for tissue engineering and regenerative medicine applications.					
34883477	6	28	theme	cartilage	1675:1683	arg1	tissue					1685:1690	native cartilage tissue	1668:1690	native cartilage tissue	1668:1690	The scaffolds were further reinforced with a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework, leading to composite implants that were more elastic than those reinforced with polycaprolactone, and which better mimicked the bulk mechanical properties of native cartilage tissue.					
34883477	1	29	theme	Biomimetic	104:113	arg1	scaffolds					115:123	Biomimetic scaffolds	104:123	Biomimetic scaffolds that provide a tissue-specific environment to cells	104:175	Biomimetic scaffolds that provide a tissue-specific environment to cells are particularly promising for tissue engineering and regenerative medicine applications.					
34883477	0	30	theme	Additive	0:7	arg1	manufacturing					9:21	Additive manufacturing	0:21	Additive manufacturing of cartilage-mimetic	0:42	Additive manufacturing of cartilage-mimetic scaffolds as off-the-shelf implants for joint regeneration.					
34883477	5	31	dep	capacity	1218:1225	arg1	enhance					1261:1267	enhance	1261:1267	to both enhance chondrogenesis of MSCs	1253:1290	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	31	dep	capacity	1218:1225	arg1	control					1299:1305	control	1299:1305	to control the release of TGF-β3	1296:1327	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	6	32	theme	native	1668:1673	arg1	tissue					1685:1690	native cartilage tissue	1668:1690	native cartilage tissue	1668:1690	The scaffolds were further reinforced with a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework, leading to composite implants that were more elastic than those reinforced with polycaprolactone, and which better mimicked the bulk mechanical properties of native cartilage tissue.					
34883477	3	33	theme	porous	597:602	arg1	scaffolds					619:627	porous alginate-based scaffolds	597:627	porous alginate-based scaffolds	597:627	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	5	34	theme	rich	1369:1372	arg1	tissue					1362:1367	a tissue	1360:1367	a tissue rich in sGAG and type II collagen	1360:1401	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	0	35	theme	cartilage-mimetic	26:42	arg1	manufacturing					9:21	Additive manufacturing	0:21	Additive manufacturing of cartilage-mimetic	0:42	Additive manufacturing of cartilage-mimetic scaffolds as off-the-shelf implants for joint regeneration.					
34883477	4	36	theme	alginate/alginate	775:791	arg1	scaffolds					801:809	the alginate/alginate sulfate scaffolds	771:809	the alginate/alginate sulfate scaffolds	771:809	Covalent crosslinking dramatically improved the elasticity of the alginate/alginate sulfate scaffolds, while scaffold architecture could be tailored using a directional freezing technique.					
34883477	2	37	theme	design	355:360	arg1	strategies					362:371	additive manufacturing and biomaterial design strategies	316:371	additive manufacturing and biomaterial design strategies	316:371	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	4	38	theme	freezing	878:885	arg1	technique					887:895	a directional freezing technique	864:895	a directional freezing technique	864:895	Covalent crosslinking dramatically improved the elasticity of the alginate/alginate sulfate scaffolds, while scaffold architecture could be tailored using a directional freezing technique.					
34883477	1	39	theme	regenerative	231:242	arg1	medicine					244:251	regenerative medicine	231:251	regenerative medicine	231:251	Biomimetic scaffolds that provide a tissue-specific environment to cells are particularly promising for tissue engineering and regenerative medicine applications.					
34883477	3	40	dep	glycosaminoglycan	539:555	arg1	mimic					564:568	mimic	564:568	mimic	564:568	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	3	41	theme	growth	682:687	arg1	TGF-β3					700:705	TGF-β3	700:705	TGF-β3	700:705	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	3	41	theme	growth	682:687	arg1	factor-β3					689:697	transforming growth factor-β3	669:697	transforming growth factor-β3 (TGF-β3)	669:706	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	2	42	theme	biomaterial	343:353	arg1	strategies					362:371	additive manufacturing and biomaterial design strategies	316:371	additive manufacturing and biomaterial design strategies	316:371	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	5	43	with	tissue	1132:1137	arg1	architecture.In					1163:1177	a biomimetic zonal architecture.In	1144:1177	a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen	1144:1401	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	2	44	theme	off-the-shelf	450:462	arg1	implants					465:472	'off-the-shelf' implants	449:472	'off-the-shelf' implants for joint regeneration	449:495	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	2	44	theme	off-the-shelf	450:462	arg1	scaffolds					417:425	articular cartilage (AC) mimetic scaffolds	384:425	articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration	384:495	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	2	45	theme	cartilage	394:402	arg1	implants					465:472	'off-the-shelf' implants	449:472	'off-the-shelf' implants for joint regeneration	449:495	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	2	45	theme	cartilage	394:402	arg1	scaffolds					417:425	articular cartilage (AC) mimetic scaffolds	384:425	articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration	384:495	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	3	46	theme	factor-β3	689:697	arg1	release					658:664	the sustained release	644:664	the sustained release of transforming growth factor-β3 (TGF-β3)	644:706	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	1	47	dep	engineering	215:225	arg1	applications					253:264	applications	253:264	applications	253:264	Biomimetic scaffolds that provide a tissue-specific environment to cells are particularly promising for tissue engineering and regenerative medicine applications.					
34883477	6	48	theme	composite	1521:1529	arg1	implants					1531:1538	composite implants	1521:1538	composite implants that were more elastic than those reinforced with polycaprolactone, and which better mimicked the bulk mechanical properties of native cartilage tissue	1521:1690	The scaffolds were further reinforced with a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework, leading to composite implants that were more elastic than those reinforced with polycaprolactone, and which better mimicked the bulk mechanical properties of native cartilage tissue.					
34883477	4	49	theme	directional	866:876	arg1	technique					887:895	a directional freezing technique	864:895	a directional freezing technique	864:895	Covalent crosslinking dramatically improved the elasticity of the alginate/alginate sulfate scaffolds, while scaffold architecture could be tailored using a directional freezing technique.					
34883477	5	50	theme	anisotropic	918:928	arg1	architecture					930:941	such an anisotropic architecture	910:941	such an anisotropic architecture	910:941	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	6	51	theme	poly	1460:1463	arg1	framework					1499:1507	a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework	1447:1507	a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework	1447:1507	The scaffolds were further reinforced with a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework, leading to composite implants that were more elastic than those reinforced with polycaprolactone, and which better mimicked the bulk mechanical properties of native cartilage tissue.					
34883477	5	52	theme	sulfated	1234:1241	arg1	scaffolds					1243:1251	the sulfated scaffolds	1230:1251	the sulfated scaffolds	1230:1251	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	7	53	theme	scaffold	1723:1730	arg1	ability					1697:1703	The ability	1693:1703	The ability of this composite scaffold to support chondrogenesis	1693:1756	The ability of this composite scaffold to support chondrogenesis was then confirmed within a dynamic culture system.					
34883477	3	54	theme	transforming	669:680	arg1	TGF-β3					700:705	TGF-β3	700:705	TGF-β3	700:705	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	3	54	theme	transforming	669:680	arg1	factor-β3					689:697	transforming growth factor-β3	669:697	transforming growth factor-β3 (TGF-β3)	669:706	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	3	55	theme	alginate-based	604:617	arg1	scaffolds					619:627	porous alginate-based scaffolds	597:627	porous alginate-based scaffolds	597:627	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	6	56	theme	mechanical	1643:1652	arg1	properties					1654:1663	the bulk mechanical properties	1634:1663	the bulk mechanical properties of native cartilage tissue	1634:1690	The scaffolds were further reinforced with a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework, leading to composite implants that were more elastic than those reinforced with polycaprolactone, and which better mimicked the bulk mechanical properties of native cartilage tissue.					
34883477	3	57	theme	end	506:508	arg1	sulfate					519:525	this end alginate sulfate	501:525	this end alginate sulfate	501:525	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	0	58	theme	off-the-shelf	57:69	arg1	implants					71:78	off-the-shelf implants	57:78	off-the-shelf implants for joint regeneration	57:101	Additive manufacturing of cartilage-mimetic scaffolds as off-the-shelf implants for joint regeneration.					
34883477	5	59	theme	scaffolds	1243:1251	arg1	capacity					1218:1225	the capacity	1214:1225	the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3	1214:1327	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	8	60	theme	scaffolds	1882:1890	arg1	potential					1853:1861	the potential	1849:1861	the potential of such biomimetic scaffolds as putative 'single-stage' or 'off-the-shelf' strategies for AC regeneration	1849:1967	Altogether, these findings demonstrate the potential of such biomimetic scaffolds as putative 'single-stage' or 'off-the-shelf' strategies for AC regeneration.					
34883477	6	61	theme	bulk	1638:1641	arg1	properties					1654:1663	the bulk mechanical properties	1634:1663	the bulk mechanical properties of native cartilage tissue	1634:1690	The scaffolds were further reinforced with a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework, leading to composite implants that were more elastic than those reinforced with polycaprolactone, and which better mimicked the bulk mechanical properties of native cartilage tissue.					
34883477	1	62	theme	tissue-specific	140:154	arg1	environment					156:166	a tissue-specific environment	138:166	a tissue-specific environment to cells	138:175	Biomimetic scaffolds that provide a tissue-specific environment to cells are particularly promising for tissue engineering and regenerative medicine applications.					
34883477	5	63	theme	extracellular	1070:1082	arg1	matrix					1084:1089	the deposited extracellular matrix	1056:1089	the deposited extracellular matrix	1056:1089	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	64	from	collagen	1394:1401	arg1	rich					1369:1372	rich	1369:1372	rich	1369:1372	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	3	65	theme	sustained	648:656	arg1	release					658:664	the sustained release	644:664	the sustained release of transforming growth factor-β3 (TGF-β3)	644:706	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	8	66	theme	biomimetic	1871:1880	arg1	scaffolds					1882:1890	such biomimetic scaffolds	1866:1890	such biomimetic scaffolds	1866:1890	Altogether, these findings demonstrate the potential of such biomimetic scaffolds as putative 'single-stage' or 'off-the-shelf' strategies for AC regeneration.					
34883477	2	67	theme	articular	384:392	arg1	cartilage					394:402	articular cartilage	384:402	articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration	384:495	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	2	67	theme	articular	384:392	arg1	AC					405:406	AC	405:406	AC	405:406	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	6	68	theme	3D	1449:1450	arg1	framework					1499:1507	a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework	1447:1507	a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework	1447:1507	The scaffolds were further reinforced with a 3D printed poly(lactide-co-ϵ-caprolactone) (PLCL) framework, leading to composite implants that were more elastic than those reinforced with polycaprolactone, and which better mimicked the bulk mechanical properties of native cartilage tissue.					
34883477	7	69	theme	composite	1713:1721	arg1	scaffold					1723:1730	this composite scaffold	1708:1730	this composite scaffold	1708:1730	The ability of this composite scaffold to support chondrogenesis was then confirmed within a dynamic culture system.					
34883477	4	70	theme	Covalent	709:716	arg1	crosslinking					718:729	Covalent crosslinking	709:729	Covalent crosslinking	709:729	Covalent crosslinking dramatically improved the elasticity of the alginate/alginate sulfate scaffolds, while scaffold architecture could be tailored using a directional freezing technique.					
34883477	7	71	theme	culture	1794:1800	arg1	system					1802:1807	a dynamic culture system	1784:1807	a dynamic culture system	1784:1807	The ability of this composite scaffold to support chondrogenesis was then confirmed within a dynamic culture system.					
34883477	3	72	theme	alginate	510:517	arg1	sulfate					519:525	this end alginate sulfate	501:525	this end alginate sulfate	501:525	To this end alginate sulfate, a sulfated glycosaminoglycan (sGAG) mimic, was used to functionalize porous alginate-based scaffolds and to support the sustained release of transforming growth factor-β3 (TGF-β3).					
34883477	0	73	theme	joint	84:88	arg1	regeneration					90:101	joint regeneration	84:101	joint regeneration	84:101	Additive manufacturing of cartilage-mimetic scaffolds as off-the-shelf implants for joint regeneration.					
34883477	5	74	theme	cartilage	1122:1130	arg1	tissue					1132:1137	cartilage tissue	1122:1137	cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen	1122:1401	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	75	theme	deposited	1060:1068	arg1	matrix					1084:1089	the deposited extracellular matrix	1056:1089	the deposited extracellular matrix	1056:1089	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	8	76	theme	single-stage	1905:1916	arg1	strategies					1938:1947	putative 'single-stage' or 'off-the-shelf' strategies	1895:1947	putative 'single-stage' or 'off-the-shelf' strategies for AC regeneration	1895:1967	Altogether, these findings demonstrate the potential of such biomimetic scaffolds as putative 'single-stage' or 'off-the-shelf' strategies for AC regeneration.					
34883477	5	77	theme	mesenchymal	964:974	arg1	MSC					987:989	MSC	987:989	MSC	987:989	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	77	theme	mesenchymal	964:974	arg1	cell					981:984	mesenchymal stem cell	964:984	mesenchymal stem cell (MSC) infiltration into the scaffold	964:1021	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	5	78	theme	tissue	1362:1367	arg1	development					1345:1355	the development	1341:1355	the development of a tissue rich in sGAG and type II collagen	1341:1401	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
34883477	2	79	theme	study	284:288	arg1	goal					271:274	The goal	267:274	The goal of this study	267:288	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	8	80	theme	such	1866:1869	arg1	scaffolds					1882:1890	such biomimetic scaffolds	1866:1890	such biomimetic scaffolds	1866:1890	Altogether, these findings demonstrate the potential of such biomimetic scaffolds as putative 'single-stage' or 'off-the-shelf' strategies for AC regeneration.					
34883477	2	81	theme	mimetic	409:415	arg1	implants					465:472	'off-the-shelf' implants	449:472	'off-the-shelf' implants for joint regeneration	449:495	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	2	81	theme	mimetic	409:415	arg1	scaffolds					417:425	articular cartilage (AC) mimetic scaffolds	384:425	articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration	384:495	The goal of this study was to integrate emerging additive manufacturing and biomaterial design strategies to produce articular cartilage (AC) mimetic scaffolds that could be used as 'off-the-shelf' implants for joint regeneration.					
34883477	5	82	theme	TGF-β3	1322:1327	arg1	release					1311:1317	the release	1307:1317	the release of TGF-β3	1307:1327	Introducing such an anisotropic architecture was found to promote mesenchymal stem cell (MSC) infiltration into the scaffold and to direct the orientation of the deposited extracellular matrix, leading to the development of cartilage tissue with a biomimetic zonal architecture.In vitroexperiments also demonstrated the capacity of the sulfated scaffolds to both enhance chondrogenesis of MSCs and to control the release of TGF-β3, leading to the development of a tissue rich in sGAG and type II collagen.					
36940514	0	0	theme	modified	93:100	arg1	proteins					115:122	modified myofibrillar proteins	93:122	modified myofibrillar proteins	93:122	Development of edible films by incorporating nanocrystalline cellulose and anthocyanins into modified myofibrillar proteins.					
36940514	5	1	theme	MP/ANT/NCC	1141:1150	arg1	MP-Lut/ANT/NCC					1153:1166	the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups	1137:1193	MP-Lut/ANT/NCC	1153:1166	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	2	with	films	814:818	arg1	group					882:886	the MP-Myr/ANT/NCC group	863:886	the MP-Myr/ANT/NCC group	863:886	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	2	with	films	814:818	arg1	interactions					849:860	stronger food component interactions	825:860	stronger food component interactions (the MP-Myr/ANT/NCC group)	825:887	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	3	theme	total	1054:1058	arg1	difference					1066:1075	total color difference	1054:1075	total color difference	1054:1075	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	3	4	dep	Fourier	481:487	arg1	transform					489:497	transform	489:497	transform infrared	489:506	Furthermore, the structure of these composite films was examined using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).					
36940514	5	5	from	break	962:966	arg1	elongation					948:957	elongation	948:957	elongation at break	948:966	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	5	from	break	962:966	arg1	strength					928:935	tensile strength	920:935	tensile strength	920:935	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	0	6	theme	myofibrillar	102:113	arg1	proteins					115:122	modified myofibrillar proteins	93:122	modified myofibrillar proteins	93:122	Development of edible films by incorporating nanocrystalline cellulose and anthocyanins into modified myofibrillar proteins.					
36940514	4	7	theme	greater	745:751	arg1	interaction					768:778	greater food component interaction	745:778	greater food component interaction	745:778	The smooth and uniform surface acquired by scanning electron microscopy (SEM) substantiated the increased compatibility and continuity observed for films with greater food component interaction.					
36940514	3	8	dep	transform	489:497	arg1	infrared					499:506	infrared	499:506	transform infrared	489:506	Furthermore, the structure of these composite films was examined using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).					
36940514	5	9	theme	MP-Que/ANT/NCC	1173:1186	arg1	groups					1188:1193	the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups	1137:1193	groups	1188:1193	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	10	dep	groups	1129:1134	arg1	MP-Lut/ANT/NCC					1153:1166	the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups	1137:1193	MP-Lut/ANT/NCC	1153:1166	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	10	dep	groups	1129:1134	arg1	groups					1188:1193	the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups	1137:1193	groups	1188:1193	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	11	theme	stronger	825:832	arg1	group					882:886	the MP-Myr/ANT/NCC group	863:886	the MP-Myr/ANT/NCC group	863:886	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	11	theme	stronger	825:832	arg1	interactions					849:860	stronger food component interactions	825:860	stronger food component interactions (the MP-Myr/ANT/NCC group)	825:887	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	4	12	theme	uniform	601:607	arg1	surface					609:615	The smooth and uniform surface	586:615	The smooth and uniform surface acquired by scanning electron microscopy (SEM)	586:662	The smooth and uniform surface acquired by scanning electron microscopy (SEM) substantiated the increased compatibility and continuity observed for films with greater food component interaction.					
36940514	5	13	dep	mechanical	908:917	arg1	elongation					948:957	elongation	948:957	elongation at break	948:966	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	13	dep	mechanical	908:917	arg1	strength					928:935	tensile strength	920:935	tensile strength	920:935	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	4	14	theme	electron	638:645	arg1	SEM					659:661	SEM	659:661	SEM	659:661	The smooth and uniform surface acquired by scanning electron microscopy (SEM) substantiated the increased compatibility and continuity observed for films with greater food component interaction.					
36940514	4	14	theme	electron	638:645	arg1	microscopy					647:656	scanning electron microscopy	629:656	scanning electron microscopy (SEM)	629:662	The smooth and uniform surface acquired by scanning electron microscopy (SEM) substantiated the increased compatibility and continuity observed for films with greater food component interaction.					
36940514	4	15	theme	component	758:766	arg1	interaction					768:778	greater food component interaction	745:778	greater food component interaction	745:778	The smooth and uniform surface acquired by scanning electron microscopy (SEM) substantiated the increased compatibility and continuity observed for films with greater food component interaction.					
36940514	3	16	theme	composite	446:454	arg1	films					456:460	these composite films	440:460	these composite films	440:460	Furthermore, the structure of these composite films was examined using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).					
36940514	3	17	theme	X-ray	517:521	arg1	XRD					536:538	XRD	536:538	XRD	536:538	Furthermore, the structure of these composite films was examined using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).					
36940514	3	17	theme	X-ray	517:521	arg1	diffraction					523:533	X-ray diffraction	517:533	X-ray diffraction (XRD)	517:539	Furthermore, the structure of these composite films was examined using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).					
36940514	3	18	theme	X-ray	546:550	arg1	XPS					580:582	XPS	580:582	XPS	580:582	Furthermore, the structure of these composite films was examined using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).					
36940514	3	18	theme	X-ray	546:550	arg1	spectroscopy					566:577	X-ray photoelectron spectroscopy	546:577	X-ray photoelectron spectroscopy (XPS)	546:583	Furthermore, the structure of these composite films was examined using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).					
36940514	2	19	dep	structure	317:325	arg1	The					313:315	The	313:315	The	313:315	The structure and rheological properties of several film-forming solutions were then determined.					
36940514	5	20	theme	MP-based	798:805	arg1	films					814:818	the MP-based edible films	794:818	the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group)	794:887	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	1	21	theme	interaction	261:271	arg1	intensity					244:252	the intensity	240:252	the intensity of the interaction of proteins with other food components	240:310	In this study, a myofibrillar protein (MP) system was used to investigate the film properties changes by adjusting the intensity of the interaction of proteins with other food components.					
36940514	1	22	with	interaction	261:271	arg1	components					301:310	other food components	290:310	other food components	290:310	In this study, a myofibrillar protein (MP) system was used to investigate the film properties changes by adjusting the intensity of the interaction of proteins with other food components.					
36940514	3	23	theme	photoelectron	552:564	arg1	XPS					580:582	XPS	580:582	XPS	580:582	Furthermore, the structure of these composite films was examined using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).					
36940514	3	23	theme	photoelectron	552:564	arg1	spectroscopy					566:577	X-ray photoelectron spectroscopy	546:577	X-ray photoelectron spectroscopy (XPS)	546:583	Furthermore, the structure of these composite films was examined using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).					
36940514	2	24	theme	solutions	378:386	arg1	structure					317:325	structure	317:325	structure	317:325	The structure and rheological properties of several film-forming solutions were then determined.					
36940514	2	24	theme	solutions	378:386	arg1	properties					343:352	rheological properties	331:352	rheological properties	331:352	The structure and rheological properties of several film-forming solutions were then determined.					
36940514	0	25	theme	films	22:26	arg1	Development					0:10	Development	0:10	Development of edible films	0:26	Development of edible films by incorporating nanocrystalline cellulose and anthocyanins into modified myofibrillar proteins.					
36940514	0	26	theme	edible	15:20	arg1	films					22:26	edible films	15:26	edible films	15:26	Development of edible films by incorporating nanocrystalline cellulose and anthocyanins into modified myofibrillar proteins.					
36940514	1	27	theme	proteins	276:283	arg1	interaction					261:271	the interaction	257:271	the interaction of proteins with other food components	257:310	In this study, a myofibrillar protein (MP) system was used to investigate the film properties changes by adjusting the intensity of the interaction of proteins with other food components.					
36940514	1	28	theme	myofibrillar	142:153	arg1	protein					155:161	myofibrillar protein	142:161	a myofibrillar protein (MP) system	140:173	In this study, a myofibrillar protein (MP) system was used to investigate the film properties changes by adjusting the intensity of the interaction of proteins with other food components.					
36940514	1	28	theme	myofibrillar	142:153	arg1	MP					164:165	MP	164:165	MP	164:165	In this study, a myofibrillar protein (MP) system was used to investigate the film properties changes by adjusting the intensity of the interaction of proteins with other food components.					
36940514	4	29	with	films	734:738	arg1	interaction					768:778	greater food component interaction	745:778	greater food component interaction	745:778	The smooth and uniform surface acquired by scanning electron microscopy (SEM) substantiated the increased compatibility and continuity observed for films with greater food component interaction.					
36940514	5	30	dep	compared	1098:1105	arg1	difference					1066:1075	total color difference	1054:1075	total color difference	1054:1075	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	31	theme	food	834:837	arg1	group					882:886	the MP-Myr/ANT/NCC group	863:886	the MP-Myr/ANT/NCC group	863:886	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	31	theme	food	834:837	arg1	interactions					849:860	stronger food component interactions	825:860	stronger food component interactions (the MP-Myr/ANT/NCC group)	825:887	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	32	theme	vapor	984:988	arg1	barrier					990:996	water vapor barrier	978:996	water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1)	978:1026	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	32	theme	vapor	984:988	arg1	10.01 × 10-9 g m-1 s-1 Pa-1					999:1025	10.01 × 10-9 g m-1 s-1 Pa-1	999:1025	10.01 × 10-9 g m-1 s-1 Pa-1	999:1025	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	33	theme	MP-Myr/ANT/NCC	867:880	arg1	group					882:886	the MP-Myr/ANT/NCC group	863:886	the MP-Myr/ANT/NCC group	863:886	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	33	theme	MP-Myr/ANT/NCC	867:880	arg1	interactions					849:860	stronger food component interactions	825:860	stronger food component interactions (the MP-Myr/ANT/NCC group)	825:887	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	3	34	theme	films	456:460	arg1	structure					427:435	the structure	423:435	the structure of these composite films	423:460	Furthermore, the structure of these composite films was examined using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).					
36940514	1	35	theme	other	290:294	arg1	components					301:310	other food components	290:310	other food components	290:310	In this study, a myofibrillar protein (MP) system was used to investigate the film properties changes by adjusting the intensity of the interaction of proteins with other food components.					
36940514	1	36	dep	properties	208:217	arg1	changes					219:225	changes	219:225	the film properties changes	199:225	In this study, a myofibrillar protein (MP) system was used to investigate the film properties changes by adjusting the intensity of the interaction of proteins with other food components.					
36940514	5	37	theme	edible	807:812	arg1	films					814:818	the MP-based edible films	794:818	the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group)	794:887	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	38	theme	component	839:847	arg1	group					882:886	the MP-Myr/ANT/NCC group	863:886	the MP-Myr/ANT/NCC group	863:886	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	38	theme	component	839:847	arg1	interactions					849:860	stronger food component interactions	825:860	stronger food component interactions (the MP-Myr/ANT/NCC group)	825:887	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	1	39	theme	film	203:206	arg1	properties					208:217	the film properties changes	199:225	the film properties changes	199:225	In this study, a myofibrillar protein (MP) system was used to investigate the film properties changes by adjusting the intensity of the interaction of proteins with other food components.					
36940514	1	40	theme	food	296:299	arg1	components					301:310	other food components	290:310	other food components	290:310	In this study, a myofibrillar protein (MP) system was used to investigate the film properties changes by adjusting the intensity of the interaction of proteins with other food components.					
36940514	5	41	theme	color	1060:1064	arg1	difference					1066:1075	total color difference	1054:1075	total color difference	1054:1075	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	2	42	theme	rheological	331:341	arg1	properties					343:352	rheological properties	331:352	rheological properties	331:352	The structure and rheological properties of several film-forming solutions were then determined.					
36940514	0	43	theme	nanocrystalline	45:59	arg1	cellulose					61:69	nanocrystalline cellulose	45:69	nanocrystalline cellulose	45:69	Development of edible films by incorporating nanocrystalline cellulose and anthocyanins into modified myofibrillar proteins.					
36940514	5	44	theme	other	1123:1127	arg1	groups					1129:1134	the other groups	1119:1134	the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups)	1119:1194	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	4	45	theme	increased	682:690	arg1	compatibility					692:704	compatibility	692:704	compatibility	692:704	The smooth and uniform surface acquired by scanning electron microscopy (SEM) substantiated the increased compatibility and continuity observed for films with greater food component interaction.					
36940514	1	46	used	used	179:182	arg2	system					168:173	a myofibrillar protein (MP) system	140:173	a myofibrillar protein (MP) system	140:173	In this study, a myofibrillar protein (MP) system was used to investigate the film properties changes by adjusting the intensity of the interaction of proteins with other food components.					
36940514	5	47	theme	tensile	920:926	arg1	elongation					948:957	elongation	948:957	elongation at break	948:966	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	47	theme	tensile	920:926	arg1	strength					928:935	tensile strength	920:935	tensile strength	920:935	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	4	48	theme	food	753:756	arg1	interaction					768:778	greater food component interaction	745:778	greater food component interaction	745:778	The smooth and uniform surface acquired by scanning electron microscopy (SEM) substantiated the increased compatibility and continuity observed for films with greater food component interaction.					
36940514	1	49	theme	protein	155:161	arg1	system					168:173	a myofibrillar protein (MP) system	140:173	a myofibrillar protein (MP) system	140:173	In this study, a myofibrillar protein (MP) system was used to investigate the film properties changes by adjusting the intensity of the interaction of proteins with other food components.					
36940514	5	50	dep	strength	928:935	arg1	%					974:974	94.43%	969:974	94.43%	969:974	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	50	dep	strength	928:935	arg1	6.68 MPa					938:945	6.68 MPa	938:945	6.68 MPa	938:945	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	51	theme	ammonia	1033:1039	arg1	sensitivity					1041:1051	ammonia sensitivity	1033:1051	ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups)	1033:1194	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	2	52	theme	film-forming	365:376	arg1	solutions					378:386	several film-forming solutions	357:386	several film-forming solutions	357:386	The structure and rheological properties of several film-forming solutions were then determined.					
36940514	4	53	theme	scanning	629:636	arg1	SEM					659:661	SEM	659:661	SEM	659:661	The smooth and uniform surface acquired by scanning electron microscopy (SEM) substantiated the increased compatibility and continuity observed for films with greater food component interaction.					
36940514	4	53	theme	scanning	629:636	arg1	microscopy					647:656	scanning electron microscopy	629:656	scanning electron microscopy (SEM)	629:662	The smooth and uniform surface acquired by scanning electron microscopy (SEM) substantiated the increased compatibility and continuity observed for films with greater food component interaction.					
36940514	5	54	dep	sensitivity	1041:1051	arg1	compared					1098:1105	compared	1098:1105	compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups)	1098:1194	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	55	theme	water	978:982	arg1	barrier					990:996	water vapor barrier	978:996	water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1)	978:1026	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	5	55	theme	water	978:982	arg1	10.01 × 10-9 g m-1 s-1 Pa-1					999:1025	10.01 × 10-9 g m-1 s-1 Pa-1	999:1025	10.01 × 10-9 g m-1 s-1 Pa-1	999:1025	In addition, the MP-based edible films with stronger food component interactions (the MP-Myr/ANT/NCC group) displayed superior mechanical (tensile strength: 6.68 MPa, elongation at break: 94.43%), water vapor barrier (10.01 × 10-9 g m-1 s-1 Pa-1), and ammonia sensitivity (total color difference: 17.00) capabilities compared to those of the other groups (the MP/ANT/NCC, MP-Lut/ANT/NCC, and MP-Que/ANT/NCC groups).					
36940514	2	56	theme	several	357:363	arg1	solutions					378:386	several film-forming solutions	357:386	several film-forming solutions	357:386	The structure and rheological properties of several film-forming solutions were then determined.					
36940514	4	57	theme	smooth	590:595	arg1	surface					609:615	The smooth and uniform surface	586:615	The smooth and uniform surface acquired by scanning electron microscopy (SEM)	586:662	The smooth and uniform surface acquired by scanning electron microscopy (SEM) substantiated the increased compatibility and continuity observed for films with greater food component interaction.					
34994000	3	0	theme	cell	351:354	arg1	density					356:362	a high cell density	344:362	a high cell density	344:362	Nevertheless, few reports are available regarding the achievement of a high cell density and good sporulation effectiveness under fermentation conditions.					
34994000	8	1	theme	MgSO4	1070:1074	arg1	O					1081:1081	MgSO4 ·7H2 O 0.012	1070:1087	MgSO4 ·7H2 O 0.012	1070:1087	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	6	2	theme	yeast	745:749	arg1	YE					760:761	YE	760:761	YE	760:761	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	6	2	theme	yeast	745:749	arg1	sources					701:707	two nitrogen sources	688:707	two nitrogen sources namely	688:714	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	6	2	theme	yeast	745:749	arg1	extract					751:757	yeast extract	745:757	yeast extract (YE)	745:762	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	1	3	theme	food	170:173	arg1	amyloliquefaciens					147:163	Bacillus amyloliquefaciens	138:163	Bacillus amyloliquefaciens	138:163	Bacillus amyloliquefaciens is a food spoilage spore-forming bacterium.					
34994000	1	3	theme	food	170:173	arg1	bacterium					198:206	a food spoilage spore-forming bacterium	168:206	a food spoilage spore-forming bacterium	168:206	Bacillus amyloliquefaciens is a food spoilage spore-forming bacterium.					
34994000	7	4	theme	MgSO4	898:902	arg1	combination					875:885	A combination	873:885	A combination of CaCl2 , MgSO4 , and MnSO4	873:914	A combination of CaCl2 , MgSO4 , and MnSO4 showed a positive impact on spores' production.					
34994000	0	5	theme	amyloliquefaciens	71:87	arg1	yields					52:57	spore yields	46:57	spore yields of Bacillus amyloliquefaciens BS13 through optimization of medium composition	46:135	Multiple linear regression models to simulate spore yields of Bacillus amyloliquefaciens BS13 through optimization of medium composition.					
34994000	4	6	theme	current	445:451	arg1	study					453:457	the current study	441:457	the current study	441:457	Therefore, the current study was designed to optimize a low-cost fermentation medium allowing the highest sporulation yield by B. amyloliquefaciens strain BS13.					
34994000	1	7	theme	spoilage	175:182	arg1	amyloliquefaciens					147:163	Bacillus amyloliquefaciens	138:163	Bacillus amyloliquefaciens	138:163	Bacillus amyloliquefaciens is a food spoilage spore-forming bacterium.					
34994000	1	7	theme	spoilage	175:182	arg1	bacterium					198:206	a food spoilage spore-forming bacterium	168:206	a food spoilage spore-forming bacterium	168:206	Bacillus amyloliquefaciens is a food spoilage spore-forming bacterium.					
34994000	11	8	theme	spore	1385:1389	arg1	preparation					1391:1401	strain BS13 spore preparation	1373:1401	strain BS13 spore preparation	1373:1401	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	11	8	theme	spore	1385:1389	arg1	agent					1463:1467	a promising probiotic and a biocontrol agent	1424:1467	agent	1463:1467	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	11	8	theme	spore	1385:1389	arg1	probiotic					1436:1444	probiotic	1436:1444	probiotic	1436:1444	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	4	9	theme	amyloliquefaciens	560:576	arg1	strain					578:583	B. amyloliquefaciens strain BS13	557:588	B. amyloliquefaciens strain BS13	557:588	Therefore, the current study was designed to optimize a low-cost fermentation medium allowing the highest sporulation yield by B. amyloliquefaciens strain BS13.					
34994000	10	10	theme	flask	1268:1272	arg1	conditions					1282:1291	flask culture conditions	1268:1291	flask culture conditions	1268:1291	The spore yield by B. amyloliquefaciens strain BS13 was enhanced from 3.0 × 1010 spores/mL under flask culture conditions to 6.2 × 1010 spores/mL when cultures were performed on large scale.					
34994000	7	11	theme	CaCl2	890:894	arg1	combination					875:885	A combination	873:885	A combination of CaCl2 , MgSO4 , and MnSO4	873:914	A combination of CaCl2 , MgSO4 , and MnSO4 showed a positive impact on spores' production.					
34994000	3	12	theme	fermentation	405:416	arg1	conditions					418:427	fermentation conditions	405:427	fermentation conditions	405:427	Nevertheless, few reports are available regarding the achievement of a high cell density and good sporulation effectiveness under fermentation conditions.					
34994000	4	13	theme	highest	528:534	arg1	yield					548:552	the highest sporulation yield	524:552	the highest sporulation yield by B. amyloliquefaciens strain BS13	524:588	Therefore, the current study was designed to optimize a low-cost fermentation medium allowing the highest sporulation yield by B. amyloliquefaciens strain BS13.					
34994000	10	14	theme	culture	1274:1280	arg1	conditions					1282:1291	flask culture conditions	1268:1291	flask culture conditions	1268:1291	The spore yield by B. amyloliquefaciens strain BS13 was enhanced from 3.0 × 1010 spores/mL under flask culture conditions to 6.2 × 1010 spores/mL when cultures were performed on large scale.					
34994000	10	15	theme	amyloliquefaciens	1193:1209	arg1	strain					1211:1216	B. amyloliquefaciens strain BS13	1190:1221	B. amyloliquefaciens strain BS13	1190:1221	The spore yield by B. amyloliquefaciens strain BS13 was enhanced from 3.0 × 1010 spores/mL under flask culture conditions to 6.2 × 1010 spores/mL when cultures were performed on large scale.					
34994000	11	16	theme	a	1450:1450	arg1	preparation					1391:1401	strain BS13 spore preparation	1373:1401	strain BS13 spore preparation	1373:1401	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	11	16	theme	a	1450:1450	arg1	agent					1463:1467	a promising probiotic and a biocontrol agent	1424:1467	agent	1463:1467	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	3	17	theme	sporulation	373:383	arg1	effectiveness					385:397	good sporulation effectiveness	368:397	good sporulation effectiveness	368:397	Nevertheless, few reports are available regarding the achievement of a high cell density and good sporulation effectiveness under fermentation conditions.					
34994000	8	18	theme	optimized	987:995	arg1	medium					997:1002	the optimized medium	983:1002	the optimized medium	983:1002	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	3	19	theme	few	289:291	arg1	reports					293:299	few reports	289:299	few reports	289:299	Nevertheless, few reports are available regarding the achievement of a high cell density and good sporulation effectiveness under fermentation conditions.					
34994000	6	20	theme	production	808:817	arg1	enhancement					787:797	a significant enhancement	773:797	a significant enhancement of spore production	773:817	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	6	21	theme	nitrogen	692:699	arg1	liquor					728:733	corn steep liquor	717:733	corn steep liquor (CSL)	717:739	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	6	21	theme	nitrogen	692:699	arg1	sources					701:707	two nitrogen sources	688:707	two nitrogen sources namely	688:714	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	6	21	theme	nitrogen	692:699	arg1	extract					751:757	yeast extract	745:757	yeast extract (YE)	745:762	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	1	22	theme	spore-forming	184:196	arg1	amyloliquefaciens					147:163	Bacillus amyloliquefaciens	138:163	Bacillus amyloliquefaciens	138:163	Bacillus amyloliquefaciens is a food spoilage spore-forming bacterium.					
34994000	1	22	theme	spore-forming	184:196	arg1	bacterium					198:206	a food spoilage spore-forming bacterium	168:206	a food spoilage spore-forming bacterium	168:206	Bacillus amyloliquefaciens is a food spoilage spore-forming bacterium.					
34994000	3	23	theme	effectiveness	385:397	arg1	achievement					329:339	the achievement	325:339	the achievement of a high cell density and good sporulation effectiveness under fermentation conditions	325:427	Nevertheless, few reports are available regarding the achievement of a high cell density and good sporulation effectiveness under fermentation conditions.					
34994000	6	24	theme	spore	802:806	arg1	production					808:817	spore production	802:817	spore production	802:817	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	10	25	theme	×	1245:1245	arg1	spores/mL					1252:1260	3.0 × 1010 spores/mL	1241:1260	3.0 × 1010 spores/mL under flask culture conditions to 6.2 × 1010 spores/mL	1241:1315	The spore yield by B. amyloliquefaciens strain BS13 was enhanced from 3.0 × 1010 spores/mL under flask culture conditions to 6.2 × 1010 spores/mL when cultures were performed on large scale.					
34994000	0	26	theme	medium	118:123	arg1	composition					125:135	medium composition	118:135	medium composition	118:135	Multiple linear regression models to simulate spore yields of Bacillus amyloliquefaciens BS13 through optimization of medium composition.					
34994000	0	27	theme	linear	9:14	arg1	models					27:32	Multiple linear regression models	0:32	Multiple linear regression models	0:32	Multiple linear regression models to simulate spore yields of Bacillus amyloliquefaciens BS13 through optimization of medium composition.					
34994000	4	28	theme	sporulation	536:546	arg1	yield					548:552	the highest sporulation yield	524:552	the highest sporulation yield by B. amyloliquefaciens strain BS13	524:588	Therefore, the current study was designed to optimize a low-cost fermentation medium allowing the highest sporulation yield by B. amyloliquefaciens strain BS13.					
34994000	10	29	theme	large	1349:1353	arg1	scale					1355:1359	large scale	1349:1359	large scale	1349:1359	The spore yield by B. amyloliquefaciens strain BS13 was enhanced from 3.0 × 1010 spores/mL under flask culture conditions to 6.2 × 1010 spores/mL when cultures were performed on large scale.					
34994000	7	30	theme	positive	925:932	arg1	impact					934:939	a positive impact	923:939	a positive impact on spores' production	923:961	A combination of CaCl2 , MgSO4 , and MnSO4 showed a positive impact on spores' production.					
34994000	0	31	theme	Multiple	0:7	arg1	models					27:32	Multiple linear regression models	0:32	Multiple linear regression models	0:32	Multiple linear regression models to simulate spore yields of Bacillus amyloliquefaciens BS13 through optimization of medium composition.					
34994000	9	32	theme	Such	1115:1118	arg1	medium					1120:1125	Such medium	1115:1125	Such medium	1115:1125	Such medium was further validated in a 400-L fermentor.					
34994000	11	33	theme	biocontrol	1452:1461	arg1	preparation					1391:1401	strain BS13 spore preparation	1373:1401	strain BS13 spore preparation	1373:1401	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	11	33	theme	biocontrol	1452:1461	arg1	agent					1463:1467	a promising probiotic and a biocontrol agent	1424:1467	agent	1463:1467	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	5	34	theme	best	643:646	arg1	carbon					648:653	carbon	648:653	carbon	648:653	Our data revealed that tryptone and starch were the best carbon and energy sources.					
34994000	6	35	theme	steep	722:726	arg1	CSL					736:738	CSL	736:738	CSL	736:738	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	6	35	theme	steep	722:726	arg1	liquor					728:733	corn steep liquor	717:733	corn steep liquor (CSL)	717:739	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	6	35	theme	steep	722:726	arg1	sources					701:707	two nitrogen sources	688:707	two nitrogen sources namely	688:714	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	10	36	theme	6.2	1296:1298	arg1	×					1300:1300	×	1300:1300	×	1300:1300	The spore yield by B. amyloliquefaciens strain BS13 was enhanced from 3.0 × 1010 spores/mL under flask culture conditions to 6.2 × 1010 spores/mL when cultures were performed on large scale.					
34994000	0	37	theme	regression	16:25	arg1	models					27:32	Multiple linear regression models	0:32	Multiple linear regression models	0:32	Multiple linear regression models to simulate spore yields of Bacillus amyloliquefaciens BS13 through optimization of medium composition.					
34994000	0	38	theme	composition	125:135	arg1	optimization					102:113	optimization	102:113	optimization of medium composition	102:135	Multiple linear regression models to simulate spore yields of Bacillus amyloliquefaciens BS13 through optimization of medium composition.					
34994000	4	39	theme	fermentation	495:506	arg1	medium					508:513	a low-cost fermentation medium	484:513	a low-cost fermentation medium allowing the highest sporulation yield by B. amyloliquefaciens strain BS13	484:588	Therefore, the current study was designed to optimize a low-cost fermentation medium allowing the highest sporulation yield by B. amyloliquefaciens strain BS13.					
34994000	6	40	theme	corn	717:720	arg1	CSL					736:738	CSL	736:738	CSL	736:738	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	6	40	theme	corn	717:720	arg1	liquor					728:733	corn steep liquor	717:733	corn steep liquor (CSL)	717:739	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	6	40	theme	corn	717:720	arg1	sources					701:707	two nitrogen sources	688:707	two nitrogen sources namely	688:714	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	10	41	theme	1010	1302:1305	arg1	×					1300:1300	×	1300:1300	×	1300:1300	The spore yield by B. amyloliquefaciens strain BS13 was enhanced from 3.0 × 1010 spores/mL under flask culture conditions to 6.2 × 1010 spores/mL when cultures were performed on large scale.					
34994000	8	42	dep	was	1004:1006	arg1	YE					1051:1052	YE 1.5	1051:1056	YE 1.5	1051:1056	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	8	42	dep	was	1004:1006	arg1	O					1105:1105	MnSO4 ·7H2 O 0.0012	1094:1112	MnSO4 ·7H2 O 0.0012	1094:1112	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	8	42	dep	was	1004:1006	arg1	g/L					1012:1014	g/L	1012:1014	g/L	1012:1014	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	8	42	dep	was	1004:1006	arg1	O					1081:1081	MgSO4 ·7H2 O 0.012	1070:1087	MgSO4 ·7H2 O 0.012	1070:1087	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	8	42	dep	was	1004:1006	arg1	CSL					1041:1043	CSL 13.5	1041:1048	CSL 13.5	1041:1048	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	8	42	dep	was	1004:1006	arg1	CaCl2					1059:1063	CaCl2 0.1	1059:1067	CaCl2 0.1	1059:1067	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	8	42	dep	was	1004:1006	arg1	tryptone					1018:1025	tryptone 3	1018:1027	tryptone 3	1018:1027	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	8	42	dep	was	1004:1006	arg1	starch					1030:1035	starch 15	1030:1038	starch 15	1030:1038	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	4	43	theme	low-cost	486:493	arg1	medium					508:513	a low-cost fermentation medium	484:513	a low-cost fermentation medium allowing the highest sporulation yield by B. amyloliquefaciens strain BS13	484:588	Therefore, the current study was designed to optimize a low-cost fermentation medium allowing the highest sporulation yield by B. amyloliquefaciens strain BS13.					
34994000	10	44	theme	B.	1190:1191	arg1	strain					1211:1216	B. amyloliquefaciens strain BS13	1190:1221	B. amyloliquefaciens strain BS13	1190:1221	The spore yield by B. amyloliquefaciens strain BS13 was enhanced from 3.0 × 1010 spores/mL under flask culture conditions to 6.2 × 1010 spores/mL when cultures were performed on large scale.					
34994000	11	45	theme	strain	1373:1378	arg1	preparation					1391:1401	strain BS13 spore preparation	1373:1401	strain BS13 spore preparation	1373:1401	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	11	45	theme	strain	1373:1378	arg1	agent					1463:1467	a promising probiotic and a biocontrol agent	1424:1467	agent	1463:1467	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	11	45	theme	strain	1373:1378	arg1	probiotic					1436:1444	probiotic	1436:1444	probiotic	1436:1444	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	2	46	theme	biotechnological	244:259	arg1	applications					261:272	multiple biotechnological applications	235:272	multiple biotechnological applications	235:272	Its spores are useful for multiple biotechnological applications.					
34994000	0	47	theme	spore	46:50	arg1	yields					52:57	spore yields	46:57	spore yields of Bacillus amyloliquefaciens BS13 through optimization of medium composition	46:135	Multiple linear regression models to simulate spore yields of Bacillus amyloliquefaciens BS13 through optimization of medium composition.					
34994000	3	48	theme	high	346:349	arg1	density					356:362	a high cell density	344:362	a high cell density	344:362	Nevertheless, few reports are available regarding the achievement of a high cell density and good sporulation effectiveness under fermentation conditions.					
34994000	6	49	theme	further	851:857	arg1	optimization					859:870	further optimization	851:870	further optimization	851:870	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	2	50	theme	multiple	235:242	arg1	applications					261:272	multiple biotechnological applications	235:272	multiple biotechnological applications	235:272	Its spores are useful for multiple biotechnological applications.					
34994000	7	51	theme	MnSO4	910:914	arg1	combination					875:885	A combination	873:885	A combination of CaCl2 , MgSO4 , and MnSO4	873:914	A combination of CaCl2 , MgSO4 , and MnSO4 showed a positive impact on spores' production.					
34994000	6	52	theme	significant	775:785	arg1	enhancement					787:797	a significant enhancement	773:797	a significant enhancement of spore production	773:817	In addition, two nitrogen sources namely, corn steep liquor (CSL) and yeast extract (YE), allowed a significant enhancement of spore production and they were both retained for further optimization.					
34994000	8	53	theme	medium	997:1002	arg1	composition					968:978	The composition	964:978	The composition of the optimized medium	964:1002	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	7	54	from	impact	934:939	arg1	production					952:961	spores' production	944:961	spores' production	944:961	A combination of CaCl2 , MgSO4 , and MnSO4 showed a positive impact on spores' production.					
34994000	11	55	theme	BS13	1380:1383	arg1	preparation					1391:1401	strain BS13 spore preparation	1373:1401	strain BS13 spore preparation	1373:1401	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	11	55	theme	BS13	1380:1383	arg1	agent					1463:1467	a promising probiotic and a biocontrol agent	1424:1467	agent	1463:1467	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	11	55	theme	BS13	1380:1383	arg1	probiotic					1436:1444	probiotic	1436:1444	probiotic	1436:1444	Therefore, strain BS13 spore preparation could be proposed as a promising probiotic and a biocontrol agent useful for plants, animals, and humans.					
34994000	5	56	dep	carbon	648:653	arg1	the					639:641	the	639:641	the	639:641	Our data revealed that tryptone and starch were the best carbon and energy sources.					
34994000	5	56	dep	carbon	648:653	arg1	sources					666:672	sources	666:672	sources	666:672	Our data revealed that tryptone and starch were the best carbon and energy sources.					
34994000	10	57	theme	spore	1175:1179	arg1	yield					1181:1185	The spore yield	1171:1185	The spore yield by B. amyloliquefaciens strain BS13	1171:1221	The spore yield by B. amyloliquefaciens strain BS13 was enhanced from 3.0 × 1010 spores/mL under flask culture conditions to 6.2 × 1010 spores/mL when cultures were performed on large scale.					
34994000	3	58	theme	density	356:362	arg1	achievement					329:339	the achievement	325:339	the achievement of a high cell density and good sporulation effectiveness under fermentation conditions	325:427	Nevertheless, few reports are available regarding the achievement of a high cell density and good sporulation effectiveness under fermentation conditions.					
34994000	9	59	theme	400-L	1154:1158	arg1	fermentor					1160:1168	a 400-L fermentor	1152:1168	a 400-L fermentor	1152:1168	Such medium was further validated in a 400-L fermentor.					
34994000	3	60	theme	good	368:371	arg1	effectiveness					385:397	good sporulation effectiveness	368:397	good sporulation effectiveness	368:397	Nevertheless, few reports are available regarding the achievement of a high cell density and good sporulation effectiveness under fermentation conditions.					
34994000	4	61	theme	B.	557:558	arg1	strain					578:583	B. amyloliquefaciens strain BS13	557:588	B. amyloliquefaciens strain BS13	557:588	Therefore, the current study was designed to optimize a low-cost fermentation medium allowing the highest sporulation yield by B. amyloliquefaciens strain BS13.					
34994000	8	62	theme	·7H2	1100:1103	arg1	O					1105:1105	MnSO4 ·7H2 O 0.0012	1094:1112	MnSO4 ·7H2 O 0.0012	1094:1112	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	8	63	theme	MnSO4	1094:1098	arg1	O					1105:1105	MnSO4 ·7H2 O 0.0012	1094:1112	MnSO4 ·7H2 O 0.0012	1094:1112	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
34994000	8	64	theme	·7H2	1076:1079	arg1	O					1081:1081	MgSO4 ·7H2 O 0.012	1070:1087	MgSO4 ·7H2 O 0.012	1070:1087	The composition of the optimized medium was (in g/L); tryptone 3, starch 15, CSL 13.5, YE 1.5, CaCl2 0.1, MgSO4 ·7H2 O 0.012, and MnSO4 ·7H2 O 0.0012.					
35882126	6	0	theme	hydrogels	808:816	arg1	correlation					828:838	their correlation	822:838	their correlation to cell biology	822:854	To date, little is known about the influence of variable agarose concentrations on the viscoelastic properties of PA hydrogels and their correlation to cell biology.					
35882126	6	0	theme	hydrogels	808:816	arg1	properties					791:800	the viscoelastic properties	774:800	the viscoelastic properties of PA hydrogels	774:816	To date, little is known about the influence of variable agarose concentrations on the viscoelastic properties of PA hydrogels and their correlation to cell biology.					
35882126	5	1	used	used	644:647	arg2	hydrogels					610:618	plasma-agarose (PA) composite hydrogels	580:618	plasma-agarose (PA) composite hydrogels	580:618	To overcome this, plasma-agarose (PA) composite hydrogels have been developed and used to prepare diverse bioengineered tissues.					
35882126	6	2	theme	viscoelastic	778:789	arg1	properties					791:800	the viscoelastic properties	774:800	the viscoelastic properties of PA hydrogels	774:816	To date, little is known about the influence of variable agarose concentrations on the viscoelastic properties of PA hydrogels and their correlation to cell biology.					
35882126	0	3	theme	Viscoelastic	0:11	arg1	properties					13:22	Viscoelastic properties	0:22	Viscoelastic properties of plasma-agarose hydrogels	0:50	Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications.					
35882126	1	4	theme	matrix	200:205	arg1	properties					168:177	the properties	164:177	the properties of the extracellular matrix (ECM)	164:211	Dermal wound healing relies on the properties of the extracellular matrix (ECM).					
35882126	10	5	theme	low	1454:1456	arg1	concentrations					1461:1474	low PA concentrations	1454:1474	low PA concentrations	1454:1474	Fibroblasts seeded in low PA concentrations spread faster during 14 d than cells cultured in higher agarose concentrations.					
35882126	8	6	theme	time	1189:1192	arg1	constants					1194:1202	relaxation time constants 1 (τ1) and 2 (τ2)	1178:1220	relaxation time constants 1 (τ1) and 2 (τ2)	1178:1220	Results show that agarose addition increased the stiffness, relaxation time constants 1 (τ1) and 2 (τ2), and fiber diameter, whereas the porosity decreased.					
35882126	3	7	from	instrumental	354:365	arg1	healing					376:382	wound healing	370:382	wound healing	370:382	After a dermal injury, a transient, biodegradable fibrin clot is instrumental in wound healing.					
35882126	0	8	theme	plasma-agarose	27:40	arg1	hydrogels					42:50	plasma-agarose hydrogels	27:50	plasma-agarose hydrogels	27:50	Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications.					
35882126	12	9	from	parameter	1713:1721	arg1	design					1730:1735	the design	1726:1735	the design of PA-based implants	1726:1756	Therefore, viscoelasticity is a key parameter in the design of PA-based implants.					
35882126	5	10	theme	bioengineered	668:680	arg1	tissues					682:688	diverse bioengineered tissues	660:688	diverse bioengineered tissues	660:688	To overcome this, plasma-agarose (PA) composite hydrogels have been developed and used to prepare diverse bioengineered tissues.					
35882126	5	11	theme	plasma-agarose	580:593	arg1	hydrogels					610:618	plasma-agarose (PA) composite hydrogels	580:618	plasma-agarose (PA) composite hydrogels	580:618	To overcome this, plasma-agarose (PA) composite hydrogels have been developed and used to prepare diverse bioengineered tissues.					
35882126	3	12	theme	dermal	297:302	arg1	injury					304:309	a dermal injury	295:309	a dermal injury	295:309	After a dermal injury, a transient, biodegradable fibrin clot is instrumental in wound healing.					
35882126	4	13	theme	main	407:410	arg1	fibrin					425:430	fibrin	425:430	fibrin	425:430	Human plasma, and its main constituent, fibrin would make a suitable biomaterial for improving wound healing and processed as hydrogels albeit with limited mechanical strength.					
35882126	4	13	theme	main	407:410	arg1	constituent					412:422	its main constituent	403:422	its main constituent	403:422	Human plasma, and its main constituent, fibrin would make a suitable biomaterial for improving wound healing and processed as hydrogels albeit with limited mechanical strength.					
35882126	7	14	from	concentrations	941:954	arg1	hydrogels					973:981	PA hydrogels	970:981	PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v)	970:1020	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	9	15	theme	fast	1382:1385	arg1	elements					1422:1429	fast (τ1) and intermediate (τ2) Maxwell elements	1382:1429	fast (τ1) and intermediate (τ2) Maxwell elements	1382:1429	Changes in cell metabolism occurred at the early stages of culturing and correlated to the displacement of fast (τ1) and intermediate (τ2) Maxwell elements.					
35882126	9	16	dep	intermediate	1396:1407	arg1	τ2					1410:1411	τ2	1410:1411	τ2	1410:1411	Changes in cell metabolism occurred at the early stages of culturing and correlated to the displacement of fast (τ1) and intermediate (τ2) Maxwell elements.					
35882126	7	17	from	agarose	959:965	arg1	hydrogels					973:981	PA hydrogels	970:981	PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v)	970:1020	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	18	dep	hydrogels	973:981	arg1	%					986:986	0 %	984:986	0 %	984:986	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	18	dep	hydrogels	973:981	arg1	%					998:998	1 %	996:998	1 %	996:998	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	18	dep	hydrogels	973:981	arg1	%					1005:1005	1.5 %	1001:1005	1.5 %	1001:1005	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	18	dep	hydrogels	973:981	arg1	w/v					1017:1019	w/v	1017:1019	w/v	1017:1019	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	18	dep	hydrogels	973:981	arg1	%					993:993	0.5 %	989:993	0.5 %	989:993	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	18	dep	hydrogels	973:981	arg1	%					1014:1014	2 %	1012:1014	2 % (w/v)	1012:1020	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	11	19	theme	PA	1597:1598	arg1	viscoelasticity					1600:1614	PA viscoelasticity	1597:1614	PA viscoelasticity	1597:1614	Collectively, these results confirm that PA viscoelasticity and hydrogel architecture strongly influenced cell behavior.					
35882126	3	20	theme	transient	314:322	arg1	fibrin					339:344	a transient, biodegradable fibrin	312:344	a transient, biodegradable fibrin clot	312:349	After a dermal injury, a transient, biodegradable fibrin clot is instrumental in wound healing.					
35882126	0	21	theme	skin	95:98	arg1	applications					119:130	skin tissue engineering applications	95:130	skin tissue engineering applications	95:130	Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications.					
35882126	7	22	theme	PA	970:971	arg1	hydrogels					973:981	PA hydrogels	970:981	PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v)	970:1020	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	0	23	theme	engineering	107:117	arg1	applications					119:130	skin tissue engineering applications	95:130	skin tissue engineering applications	95:130	Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications.					
35882126	7	24	theme	agarose	959:965	arg1	concentrations					941:954	different concentrations	931:954	different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v)	931:1020	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	8	25	dep	constants	1194:1202	arg1	τ2					1218:1219	τ2	1218:1219	τ2	1218:1219	Results show that agarose addition increased the stiffness, relaxation time constants 1 (τ1) and 2 (τ2), and fiber diameter, whereas the porosity decreased.					
35882126	8	25	dep	constants	1194:1202	arg1	τ1					1207:1208	τ1	1207:1208	τ1	1207:1208	Results show that agarose addition increased the stiffness, relaxation time constants 1 (τ1) and 2 (τ2), and fiber diameter, whereas the porosity decreased.					
35882126	8	25	dep	constants	1194:1202	arg1	2					1215:1215	2	1215:1215	2	1215:1215	Results show that agarose addition increased the stiffness, relaxation time constants 1 (τ1) and 2 (τ2), and fiber diameter, whereas the porosity decreased.					
35882126	8	25	dep	constants	1194:1202	arg1	1					1204:1204	1	1204:1204	1	1204:1204	Results show that agarose addition increased the stiffness, relaxation time constants 1 (τ1) and 2 (τ2), and fiber diameter, whereas the porosity decreased.					
35882126	7	26	from	activity	1080:1087	arg1	characterization					880:895	the characterization	876:895	the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts	876:1115	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	27	theme	fibroblasts	1105:1115	arg1	number					1055:1060	the cell number	1046:1060	the cell number	1046:1060	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	27	theme	fibroblasts	1105:1115	arg1	activity					1080:1087	mitochondrial activity	1066:1087	mitochondrial activity	1066:1087	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	28	theme	concentrations	941:954	arg1	influence					1033:1041	their influence	1027:1041	their influence on the cell number and mitochondrial activity of human dermal fibroblasts	1027:1115	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	28	theme	concentrations	941:954	arg1	properties					917:926	the viscoelastic properties	900:926	the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v)	900:1020	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	29	theme	human	1092:1096	arg1	fibroblasts					1105:1115	human dermal fibroblasts	1092:1115	human dermal fibroblasts	1092:1115	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	9	30	theme	elements	1422:1429	arg1	displacement					1366:1377	the displacement	1362:1377	the displacement of fast (τ1) and intermediate (τ2) Maxwell elements	1362:1429	Changes in cell metabolism occurred at the early stages of culturing and correlated to the displacement of fast (τ1) and intermediate (τ2) Maxwell elements.					
35882126	3	31	theme	wound	370:374	arg1	healing					376:382	wound healing	370:382	wound healing	370:382	After a dermal injury, a transient, biodegradable fibrin clot is instrumental in wound healing.					
35882126	4	32	theme	mechanical	541:550	arg1	strength					552:559	limited mechanical strength	533:559	limited mechanical strength	533:559	Human plasma, and its main constituent, fibrin would make a suitable biomaterial for improving wound healing and processed as hydrogels albeit with limited mechanical strength.					
35882126	3	33	dep	transient	314:322	arg1	biodegradable					325:337	biodegradable	325:337	biodegradable	325:337	After a dermal injury, a transient, biodegradable fibrin clot is instrumental in wound healing.					
35882126	10	34	theme	agarose	1532:1538	arg1	concentrations					1540:1553	higher agarose concentrations	1525:1553	higher agarose concentrations	1525:1553	Fibroblasts seeded in low PA concentrations spread faster during 14 d than cells cultured in higher agarose concentrations.					
35882126	1	35	theme	Dermal	133:138	arg1	healing					146:152	Dermal wound healing	133:152	Dermal wound healing	133:152	Dermal wound healing relies on the properties of the extracellular matrix (ECM).					
35882126	0	36	theme	hydrogels	42:50	arg1	properties					13:22	Viscoelastic properties	0:22	Viscoelastic properties of plasma-agarose hydrogels	0:50	Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications.					
35882126	12	37	theme	implants	1749:1756	arg1	design					1730:1735	the design	1726:1735	the design of PA-based implants	1726:1756	Therefore, viscoelasticity is a key parameter in the design of PA-based implants.					
35882126	6	38	theme	variable	739:746	arg1	concentrations					756:769	variable agarose concentrations	739:769	variable agarose concentrations	739:769	To date, little is known about the influence of variable agarose concentrations on the viscoelastic properties of PA hydrogels and their correlation to cell biology.					
35882126	7	39	theme	cell	1050:1053	arg1	number					1055:1060	the cell number	1046:1060	the cell number	1046:1060	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	12	40	theme	key	1709:1711	arg1	parameter					1713:1721	a key parameter	1707:1721	a key parameter in the design of PA-based implants	1707:1756	Therefore, viscoelasticity is a key parameter in the design of PA-based implants.					
35882126	12	40	theme	key	1709:1711	arg1	viscoelasticity					1688:1702	viscoelasticity	1688:1702	viscoelasticity	1688:1702	Therefore, viscoelasticity is a key parameter in the design of PA-based implants.					
35882126	6	41	from	influence	726:734	arg1	correlation					828:838	their correlation	822:838	their correlation to cell biology	822:854	To date, little is known about the influence of variable agarose concentrations on the viscoelastic properties of PA hydrogels and their correlation to cell biology.					
35882126	6	41	from	influence	726:734	arg1	properties					791:800	the viscoelastic properties	774:800	the viscoelastic properties of PA hydrogels	774:816	To date, little is known about the influence of variable agarose concentrations on the viscoelastic properties of PA hydrogels and their correlation to cell biology.					
35882126	7	42	theme	properties	917:926	arg1	characterization					880:895	the characterization	876:895	the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts	876:1115	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	9	43	theme	early	1318:1322	arg1	stages					1324:1329	the early stages	1314:1329	the early stages of culturing	1314:1342	Changes in cell metabolism occurred at the early stages of culturing and correlated to the displacement of fast (τ1) and intermediate (τ2) Maxwell elements.					
35882126	7	44	theme	influence	1033:1041	arg1	characterization					880:895	the characterization	876:895	the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts	876:1115	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	9	45	theme	cell	1286:1289	arg1	metabolism					1291:1300	cell metabolism	1286:1300	cell metabolism	1286:1300	Changes in cell metabolism occurred at the early stages of culturing and correlated to the displacement of fast (τ1) and intermediate (τ2) Maxwell elements.					
35882126	6	46	theme	PA	805:806	arg1	hydrogels					808:816	PA hydrogels	805:816	PA hydrogels	805:816	To date, little is known about the influence of variable agarose concentrations on the viscoelastic properties of PA hydrogels and their correlation to cell biology.					
35882126	1	47	theme	extracellular	186:198	arg1	ECM					208:210	ECM	208:210	ECM	208:210	Dermal wound healing relies on the properties of the extracellular matrix (ECM).					
35882126	1	47	theme	extracellular	186:198	arg1	matrix					200:205	the extracellular matrix	182:205	the extracellular matrix (ECM)	182:211	Dermal wound healing relies on the properties of the extracellular matrix (ECM).					
35882126	0	48	theme	favorable	60:68	arg1	responses					81:89	favorable fibroblast responses	60:89	favorable fibroblast responses for skin tissue engineering applications	60:130	Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications.					
35882126	4	49	theme	limited	533:539	arg1	strength					552:559	limited mechanical strength	533:559	limited mechanical strength	533:559	Human plasma, and its main constituent, fibrin would make a suitable biomaterial for improving wound healing and processed as hydrogels albeit with limited mechanical strength.					
35882126	10	50	theme	PA	1458:1459	arg1	concentrations					1461:1474	low PA concentrations	1454:1474	low PA concentrations	1454:1474	Fibroblasts seeded in low PA concentrations spread faster during 14 d than cells cultured in higher agarose concentrations.					
35882126	4	51	theme	Human	385:389	arg1	plasma					391:396	Human plasma	385:396	Human plasma	385:396	Human plasma, and its main constituent, fibrin would make a suitable biomaterial for improving wound healing and processed as hydrogels albeit with limited mechanical strength.					
35882126	6	52	theme	concentrations	756:769	arg1	influence					726:734	the influence	722:734	the influence of variable agarose concentrations on the viscoelastic properties of PA hydrogels and their correlation to cell biology	722:854	To date, little is known about the influence of variable agarose concentrations on the viscoelastic properties of PA hydrogels and their correlation to cell biology.					
35882126	9	53	theme	culturing	1334:1342	arg1	stages					1324:1329	the early stages	1314:1329	the early stages of culturing	1314:1342	Changes in cell metabolism occurred at the early stages of culturing and correlated to the displacement of fast (τ1) and intermediate (τ2) Maxwell elements.					
35882126	8	54	theme	relaxation	1178:1187	arg1	constants					1194:1202	relaxation time constants 1 (τ1) and 2 (τ2)	1178:1220	relaxation time constants 1 (τ1) and 2 (τ2)	1178:1220	Results show that agarose addition increased the stiffness, relaxation time constants 1 (τ1) and 2 (τ2), and fiber diameter, whereas the porosity decreased.					
35882126	2	55	theme	skin	245:248	arg1	ECM					250:252	skin ECM	245:252	skin ECM	245:252	Thus, hydrogels that replicate skin ECM have reached clinical application.					
35882126	4	56	theme	suitable	445:452	arg1	biomaterial					454:464	a suitable biomaterial	443:464	a suitable biomaterial for improving wound healing and processed as hydrogels albeit with limited mechanical strength	443:559	Human plasma, and its main constituent, fibrin would make a suitable biomaterial for improving wound healing and processed as hydrogels albeit with limited mechanical strength.					
35882126	5	57	theme	diverse	660:666	arg1	tissues					682:688	diverse bioengineered tissues	660:688	diverse bioengineered tissues	660:688	To overcome this, plasma-agarose (PA) composite hydrogels have been developed and used to prepare diverse bioengineered tissues.					
35882126	1	58	theme	wound	140:144	arg1	healing					146:152	Dermal wound healing	133:152	Dermal wound healing	133:152	Dermal wound healing relies on the properties of the extracellular matrix (ECM).					
35882126	8	59	theme	agarose	1136:1142	arg1	addition					1144:1151	agarose addition	1136:1151	agarose addition	1136:1151	Results show that agarose addition increased the stiffness, relaxation time constants 1 (τ1) and 2 (τ2), and fiber diameter, whereas the porosity decreased.					
35882126	2	60	theme	clinical	267:274	arg1	application					276:286	clinical application	267:286	clinical application	267:286	Thus, hydrogels that replicate skin ECM have reached clinical application.					
35882126	11	61	theme	hydrogel	1620:1627	arg1	architecture					1629:1640	hydrogel architecture	1620:1640	hydrogel architecture	1620:1640	Collectively, these results confirm that PA viscoelasticity and hydrogel architecture strongly influenced cell behavior.					
35882126	3	62	theme	fibrin	339:344	arg1	clot					346:349	a transient, biodegradable fibrin clot	312:349	a transient, biodegradable fibrin clot	312:349	After a dermal injury, a transient, biodegradable fibrin clot is instrumental in wound healing.					
35882126	0	63	theme	tissue	100:105	arg1	applications					119:130	skin tissue engineering applications	95:130	skin tissue engineering applications	95:130	Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications.					
35882126	7	64	from	number	1055:1060	arg1	characterization					880:895	the characterization	876:895	the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts	876:1115	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	65	from	hydrogels	973:981	arg1	concentrations					941:954	different concentrations	931:954	different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v)	931:1020	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	11	66	theme	cell	1662:1665	arg1	behavior					1667:1674	cell behavior	1662:1674	cell behavior	1662:1674	Collectively, these results confirm that PA viscoelasticity and hydrogel architecture strongly influenced cell behavior.					
35882126	9	67	theme	intermediate	1396:1407	arg1	elements					1422:1429	fast (τ1) and intermediate (τ2) Maxwell elements	1382:1429	fast (τ1) and intermediate (τ2) Maxwell elements	1382:1429	Changes in cell metabolism occurred at the early stages of culturing and correlated to the displacement of fast (τ1) and intermediate (τ2) Maxwell elements.					
35882126	9	68	dep	fast	1382:1385	arg1	τ1					1388:1389	τ1	1388:1389	τ1	1388:1389	Changes in cell metabolism occurred at the early stages of culturing and correlated to the displacement of fast (τ1) and intermediate (τ2) Maxwell elements.					
35882126	7	69	theme	dermal	1098:1103	arg1	fibroblasts					1105:1115	human dermal fibroblasts	1092:1115	human dermal fibroblasts	1092:1115	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	3	70	from	healing	376:382	arg1	instrumental					354:365	instrumental	354:365	instrumental	354:365	After a dermal injury, a transient, biodegradable fibrin clot is instrumental in wound healing.					
35882126	5	71	theme	composite	600:608	arg1	hydrogels					610:618	plasma-agarose (PA) composite hydrogels	580:618	plasma-agarose (PA) composite hydrogels	580:618	To overcome this, plasma-agarose (PA) composite hydrogels have been developed and used to prepare diverse bioengineered tissues.					
35882126	4	72	theme	wound	480:484	arg1	healing					486:492	wound healing	480:492	wound healing	480:492	Human plasma, and its main constituent, fibrin would make a suitable biomaterial for improving wound healing and processed as hydrogels albeit with limited mechanical strength.					
35882126	8	73	theme	fiber	1227:1231	arg1	diameter					1233:1240	fiber diameter	1227:1240	fiber diameter	1227:1240	Results show that agarose addition increased the stiffness, relaxation time constants 1 (τ1) and 2 (τ2), and fiber diameter, whereas the porosity decreased.					
35882126	9	74	theme	Maxwell	1414:1420	arg1	elements					1422:1429	fast (τ1) and intermediate (τ2) Maxwell elements	1382:1429	fast (τ1) and intermediate (τ2) Maxwell elements	1382:1429	Changes in cell metabolism occurred at the early stages of culturing and correlated to the displacement of fast (τ1) and intermediate (τ2) Maxwell elements.					
35882126	7	75	theme	mitochondrial	1066:1078	arg1	activity					1080:1087	mitochondrial activity	1066:1087	mitochondrial activity	1066:1087	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	12	76	theme	PA-based	1740:1747	arg1	implants					1749:1756	PA-based implants	1740:1756	PA-based implants	1740:1756	Therefore, viscoelasticity is a key parameter in the design of PA-based implants.					
35882126	7	77	theme	different	931:939	arg1	concentrations					941:954	different concentrations	931:954	different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v)	931:1020	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	78	from	properties	917:926	arg1	number					1055:1060	the cell number	1046:1060	the cell number	1046:1060	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	78	from	properties	917:926	arg1	activity					1080:1087	mitochondrial activity	1066:1087	mitochondrial activity	1066:1087	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	10	79	theme	higher	1525:1530	arg1	concentrations					1540:1553	higher agarose concentrations	1525:1553	higher agarose concentrations	1525:1553	Fibroblasts seeded in low PA concentrations spread faster during 14 d than cells cultured in higher agarose concentrations.					
35882126	6	80	theme	agarose	748:754	arg1	concentrations					756:769	variable agarose concentrations	739:769	variable agarose concentrations	739:769	To date, little is known about the influence of variable agarose concentrations on the viscoelastic properties of PA hydrogels and their correlation to cell biology.					
35882126	0	81	theme	fibroblast	70:79	arg1	responses					81:89	favorable fibroblast responses	60:89	favorable fibroblast responses for skin tissue engineering applications	60:130	Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications.					
35882126	9	82	from	Changes	1275:1281	arg1	metabolism					1291:1300	cell metabolism	1286:1300	cell metabolism	1286:1300	Changes in cell metabolism occurred at the early stages of culturing and correlated to the displacement of fast (τ1) and intermediate (τ2) Maxwell elements.					
35882126	6	83	theme	cell	843:846	arg1	biology					848:854	cell biology	843:854	cell biology	843:854	To date, little is known about the influence of variable agarose concentrations on the viscoelastic properties of PA hydrogels and their correlation to cell biology.					
35882126	7	84	from	influence	1033:1041	arg1	number					1055:1060	the cell number	1046:1060	the cell number	1046:1060	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	84	from	influence	1033:1041	arg1	activity					1080:1087	mitochondrial activity	1066:1087	mitochondrial activity	1066:1087	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	85	theme	viscoelastic	904:915	arg1	properties					917:926	the viscoelastic properties	900:926	the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v)	900:1020	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	86	from	characterization	880:895	arg1	number					1055:1060	the cell number	1046:1060	the cell number	1046:1060	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
35882126	7	86	from	characterization	880:895	arg1	activity					1080:1087	mitochondrial activity	1066:1087	mitochondrial activity	1066:1087	This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts.					
36943336	8	0	theme	same	898:901	arg1	lineage					903:909	the same lineage	894:909	the same lineage	894:909	In phylogenetic analysis, strain DA-C8T belonged to the same lineage as Xylanibacillus composti K13T (92.5 %), but there was less statistical support for branching (70 %).					
36943336	12	1	theme	major	1523:1527	arg1	lipids					1535:1540	the major polar lipids	1519:1540	the major polar lipids	1519:1540	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	12	1	theme	major	1523:1527	arg1	glycolipids					1560:1570	unidentified glycolipids	1547:1570	unidentified glycolipids	1547:1570	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	9	2	theme	amino	1092:1096	arg1	identity					1112:1119	average amino acid sequence identity	1084:1119	average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT	1084:1170	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	9	3	dep	P.	1147:1148	arg1	cisolokensis					1150:1161	cisolokensis	1150:1161	cisolokensis	1150:1161	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	7	4	theme	sequences	679:687	arg1	Comparison					651:660	Comparison	651:660	Comparison of 16S rRNA gene sequences	651:687	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	3	5	theme	beech	331:335	arg1	source					367:372	the sole carbon source	351:372	the sole carbon source	351:372	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
36943336	3	5	theme	beech	331:335	arg1	xylan					342:346	beech wood xylan	331:346	beech wood xylan	331:346	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
36943336	12	6	theme	major	1373:1377	arg1	C16 					1405:1408	C16 	1405:1408	C16 	1405:1408	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	12	6	theme	major	1373:1377	arg1	acids					1394:1398	The major cellular fatty acids	1369:1398	The major cellular fatty acids	1369:1398	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	4	7	theme	high	393:396	arg1	ability					416:422	high xylan degradation ability	393:422	high xylan degradation ability	393:422	The strain showed high xylan degradation ability under anaerobic growth conditions.					
36943336	9	8	theme	21.8	1177:1180	arg1	%					1197:1197	21.8, 68.3 and 58.2 %	1177:1197	%	1197:1197	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	11	9	theme	52.3 mol	1358:1365	arg1	content					1329:1335	The whole-genome DNA G+C content	1304:1335	The whole-genome DNA G+C content of strain DA-C8T	1304:1352	The whole-genome DNA G+C content of strain DA-C8T was 52.3 mol%.					
36943336	11	9	theme	52.3 mol	1358:1365	arg1	%					1366:1366	52.3 mol%	1358:1366	52.3 mol%	1358:1366	The whole-genome DNA G+C content of strain DA-C8T was 52.3 mol%.					
36943336	3	10	theme	sole	355:358	arg1	source					367:372	the sole carbon source	351:372	the sole carbon source	351:372	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
36943336	3	10	theme	sole	355:358	arg1	xylan					342:346	beech wood xylan	331:346	beech wood xylan	331:346	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
36943336	13	11	dep	evidence	1633:1640	arg1	basis					1580:1584	basis	1580:1584	basis	1580:1584	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	13	11	dep	evidence	1633:1640	arg1	the					1576:1578	the	1576:1578	the	1576:1578	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	9	12	theme	sequence	1103:1110	arg1	identity					1112:1119	average amino acid sequence identity	1084:1119	average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT	1084:1170	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	1	13	theme	xylanolytic	57:67	arg1	bacterium					69:77	a thermophilic xylanolytic bacterium	42:77	a thermophilic xylanolytic bacterium	42:77	nov., a thermophilic xylanolytic bacterium isolated from compost.					
36943336	1	13	theme	xylanolytic	57:67	arg1	nov.					36:39	nov.	36:39	nov.	36:39	nov., a thermophilic xylanolytic bacterium isolated from compost.					
36943336	13	14	theme	novel	1701:1705	arg1	sp					1747:1748	the novel species Insulambacter thermoxylanivorax sp	1697:1748	proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp	1660:1748	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	7	15	theme	rRNA	669:672	arg1	sequences					679:687	16S rRNA gene sequences	665:687	16S rRNA gene sequences	665:687	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	3	16	theme	carbon	360:365	arg1	source					367:372	the sole carbon source	351:372	the sole carbon source	351:372	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
36943336	3	16	theme	carbon	360:365	arg1	xylan					342:346	beech wood xylan	331:346	beech wood xylan	331:346	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
36943336	9	17	theme	average	1045:1051	arg1	identity					1064:1071	average nucleotide identity	1045:1071	average nucleotide identity	1045:1071	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	9	17	theme	average	1045:1051	arg1	hybridization					1030:1042	Digital DNA-DNA hybridization	1014:1042	Digital DNA-DNA hybridization	1014:1042	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	4	18	theme	degradation	404:414	arg1	ability					416:422	high xylan degradation ability	393:422	high xylan degradation ability	393:422	The strain showed high xylan degradation ability under anaerobic growth conditions.					
36943336	11	19	theme	strain	1340:1345	arg1	content					1329:1335	The whole-genome DNA G+C content	1304:1335	The whole-genome DNA G+C content of strain DA-C8T	1304:1352	The whole-genome DNA G+C content of strain DA-C8T was 52.3 mol%.					
36943336	11	19	theme	strain	1340:1345	arg1	%					1366:1366	52.3 mol%	1358:1366	52.3 mol%	1358:1366	The whole-genome DNA G+C content of strain DA-C8T was 52.3 mol%.					
36943336	13	20	theme	novel	1645:1649	arg1	genus					1651:1655	a novel genus	1643:1655	a novel genus	1643:1655	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	13	20	theme	novel	1645:1649	arg1	nov.-for					1688:1695	proposed-Insulambacter gen. nov.-for	1660:1695	proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp	1660:1748	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	7	21	theme	gene	674:677	arg1	sequences					679:687	16S rRNA gene sequences	665:687	16S rRNA gene sequences	665:687	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	8	22	dep	was	963:965	arg1	%					1010:1010	70 %	1007:1010	70 %	1007:1010	In phylogenetic analysis, strain DA-C8T belonged to the same lineage as Xylanibacillus composti K13T (92.5 %), but there was less statistical support for branching (70 %).					
36943336	2	23	theme	thermophilic	158:169	arg1	bacterium					188:196	a Gram-negative, facultatively thermophilic, xylan-degrading bacterium	127:196	bacterium	188:196	We isolated and analysed a Gram-negative, facultatively thermophilic, xylan-degrading bacterium that we designated as strain DA-C8T.					
36943336	4	24	theme	xylan	398:402	arg1	ability					416:422	high xylan degradation ability	393:422	high xylan degradation ability	393:422	The strain showed high xylan degradation ability under anaerobic growth conditions.					
36943336	10	25	theme	strain	1228:1233	arg1	DA-C8T					1235:1240	strain DA-C8T	1228:1240	strain DA-C8T	1228:1240	Those between strain DA-C8T and X. composti K13 were 23.7, 67.7 and 57.6 %, respectively.					
36943336	9	26	theme	acid	1098:1101	arg1	identity					1112:1119	average amino acid sequence identity	1084:1119	average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT	1084:1170	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	8	27	theme	statistical	972:982	arg1	support					984:990	less statistical support	967:990	less statistical support for branching	967:1004	In phylogenetic analysis, strain DA-C8T belonged to the same lineage as Xylanibacillus composti K13T (92.5 %), but there was less statistical support for branching (70 %).					
36943336	9	28	theme	strain	1129:1134	arg1	DA-C8T					1136:1141	strain DA-C8T	1129:1141	strain DA-C8T	1129:1141	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	7	29	theme	strain	704:709	arg1	DA-C8T					711:716	strain DA-C8T	704:716	strain DA-C8T	704:716	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	5	30	dep	optimum	522:528	arg1	pH					531:532	pH 9.0	531:536	pH 9.0	531:536	The isolate grew at 37-60 °C (optimum, 55 °C) and pH 4.0-11.0 (optimum, pH 9.0).					
36943336	0	31	theme	thermoxylanivorax	14:30	arg1	sp					32:33	Insulambacter thermoxylanivorax sp	0:33	Insulambacter thermoxylanivorax sp.	0:34	Insulambacter thermoxylanivorax sp.					
36943336	13	32	theme	evidence	1633:1640	arg1	genus					1651:1655	a novel genus	1643:1655	a novel genus	1643:1655	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	13	32	theme	evidence	1633:1640	arg1	nov.-for					1688:1695	proposed-Insulambacter gen. nov.-for	1660:1695	proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp	1660:1748	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	13	33	theme	phenotypic	1589:1598	arg1	evidence					1633:1640	phenotypic, chemotaxonomic and phylogenetic evidence	1589:1640	phenotypic, chemotaxonomic and phylogenetic evidence	1589:1640	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	13	34	theme	chemotaxonomic	1601:1614	arg1	evidence					1633:1640	phenotypic, chemotaxonomic and phylogenetic evidence	1589:1640	phenotypic, chemotaxonomic and phylogenetic evidence	1589:1640	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	7	35	theme	chitinolyticus	809:822	arg1	HSCC596					824:830	Paenibacillus chitinolyticus HSCC596	795:830	Paenibacillus chitinolyticus HSCC596 (93.5 %)	795:839	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	7	35	theme	chitinolyticus	809:822	arg1	%					838:838	93.5 %	833:838	93.5 %	833:838	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	0	36	theme	Insulambacter	0:12	arg1	sp					32:33	Insulambacter thermoxylanivorax sp	0:33	Insulambacter thermoxylanivorax sp.	0:34	Insulambacter thermoxylanivorax sp.					
36943336	9	37	theme	58.2 	1192:1196	arg1	%					1197:1197	21.8, 68.3 and 58.2 %	1177:1197	%	1197:1197	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	8	38	theme	phylogenetic	845:856	arg1	analysis					858:865	phylogenetic analysis	845:865	phylogenetic analysis	845:865	In phylogenetic analysis, strain DA-C8T belonged to the same lineage as Xylanibacillus composti K13T (92.5 %), but there was less statistical support for branching (70 %).					
36943336	12	39	theme	cellular	1379:1386	arg1	C16 					1405:1408	C16 	1405:1408	C16 	1405:1408	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	12	39	theme	cellular	1379:1386	arg1	acids					1394:1398	The major cellular fatty acids	1369:1398	The major cellular fatty acids	1369:1398	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	9	40	theme	P.	1147:1148	arg1	LC2-13AT					1163:1170	P. cisolokensis LC2-13AT	1147:1170	P. cisolokensis LC2-13AT	1147:1170	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	6	41	theme	carbon	635:640	arg1	galactan					623:630	galactan	623:630	galactan	623:630	As well as xylan, strain DA-C8T could use polysaccharides such as arabinoxylan and galactan as carbon sources.					
36943336	6	41	theme	carbon	635:640	arg1	sources					642:648	carbon sources	635:648	carbon sources	635:648	As well as xylan, strain DA-C8T could use polysaccharides such as arabinoxylan and galactan as carbon sources.					
36943336	6	41	theme	carbon	635:640	arg1	arabinoxylan					606:617	arabinoxylan	606:617	arabinoxylan	606:617	As well as xylan, strain DA-C8T could use polysaccharides such as arabinoxylan and galactan as carbon sources.					
36943336	6	41	theme	carbon	635:640	arg1	polysaccharides					582:596	polysaccharides	582:596	polysaccharides such as arabinoxylan and galactan	582:630	As well as xylan, strain DA-C8T could use polysaccharides such as arabinoxylan and galactan as carbon sources.					
36943336	7	42	theme	Paenibacillus	746:758	arg1	LC2-13AT					773:780	Paenibacillus cisolokensis LC2-13AT	746:780	Paenibacillus cisolokensis LC2-13AT (93.9 %)	746:789	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	7	42	theme	Paenibacillus	746:758	arg1	%					788:788	93.9 %	783:788	93.9 %	783:788	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	10	43	theme	X.	1246:1247	arg1	K13					1258:1260	X. composti K13	1246:1260	X. composti K13	1246:1260	Those between strain DA-C8T and X. composti K13 were 23.7, 67.7 and 57.6 %, respectively.					
36943336	11	44	theme	whole-genome	1308:1319	arg1	content					1329:1335	The whole-genome DNA G+C content	1304:1335	The whole-genome DNA G+C content of strain DA-C8T	1304:1352	The whole-genome DNA G+C content of strain DA-C8T was 52.3 mol%.					
36943336	11	44	theme	whole-genome	1308:1319	arg1	%					1366:1366	52.3 mol%	1358:1366	52.3 mol%	1358:1366	The whole-genome DNA G+C content of strain DA-C8T was 52.3 mol%.					
36943336	11	45	theme	DNA	1321:1323	arg1	content					1329:1335	The whole-genome DNA G+C content	1304:1335	The whole-genome DNA G+C content of strain DA-C8T	1304:1352	The whole-genome DNA G+C content of strain DA-C8T was 52.3 mol%.					
36943336	11	45	theme	DNA	1321:1323	arg1	%					1366:1366	52.3 mol%	1358:1366	52.3 mol%	1358:1366	The whole-genome DNA G+C content of strain DA-C8T was 52.3 mol%.					
36943336	9	46	theme	DNA-DNA	1022:1028	arg1	identity					1064:1071	average nucleotide identity	1045:1071	average nucleotide identity	1045:1071	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	9	46	theme	DNA-DNA	1022:1028	arg1	hybridization					1030:1042	Digital DNA-DNA hybridization	1014:1042	Digital DNA-DNA hybridization	1014:1042	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	13	47	theme	phylogenetic	1620:1631	arg1	evidence					1633:1640	phenotypic, chemotaxonomic and phylogenetic evidence	1589:1640	phenotypic, chemotaxonomic and phylogenetic evidence	1589:1640	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	7	48	theme	cisolokensis	760:771	arg1	LC2-13AT					773:780	Paenibacillus cisolokensis LC2-13AT	746:780	Paenibacillus cisolokensis LC2-13AT (93.9 %)	746:789	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	7	48	theme	cisolokensis	760:771	arg1	%					788:788	93.9 %	783:788	93.9 %	783:788	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	3	49	theme	Ishigaki	277:284	arg1	Island					286:291	Ishigaki Island	277:291	Ishigaki Island	277:291	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
36943336	7	50	theme	16S	665:667	arg1	sequences					679:687	16S rRNA gene sequences	665:687	16S rRNA gene sequences	665:687	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	9	51	theme	hybridization	1030:1042	arg1	values					1073:1078	Digital DNA-DNA hybridization, average nucleotide identity values	1014:1078	Digital DNA-DNA hybridization, average nucleotide identity values	1014:1078	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	13	52	theme	proposed-Insulambacter	1660:1681	arg1	genus					1651:1655	a novel genus	1643:1655	a novel genus	1643:1655	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	13	52	theme	proposed-Insulambacter	1660:1681	arg1	nov.-for					1688:1695	proposed-Insulambacter gen. nov.-for	1660:1695	proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp	1660:1748	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	15	53	theme	type	1760:1763	arg1	strain					1765:1770	The type strain	1756:1770	The type strain	1756:1770	The type strain is DA-C8T (=JCM 34211T=DSM 111723T).					
36943336	15	53	theme	type	1760:1763	arg1	DA-C8T					1775:1780	DA-C8T	1775:1780	DA-C8T (=JCM 34211T=DSM 111723T)	1775:1806	The type strain is DA-C8T (=JCM 34211T=DSM 111723T).					
36943336	3	54	theme	enrichment	304:313	arg1	culturing					315:323	enrichment culturing	304:323	enrichment culturing using beech wood xylan as the sole carbon source	304:372	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
36943336	2	55	theme	xylan-degrading	172:186	arg1	bacterium					188:196	a Gram-negative, facultatively thermophilic, xylan-degrading bacterium	127:196	bacterium	188:196	We isolated and analysed a Gram-negative, facultatively thermophilic, xylan-degrading bacterium that we designated as strain DA-C8T.					
36943336	8	56	theme	strain	868:873	arg1	DA-C8T					875:880	strain DA-C8T	868:880	strain DA-C8T	868:880	In phylogenetic analysis, strain DA-C8T belonged to the same lineage as Xylanibacillus composti K13T (92.5 %), but there was less statistical support for branching (70 %).					
36943336	12	57	theme	unidentified	1547:1558	arg1	lipids					1535:1540	the major polar lipids	1519:1540	the major polar lipids	1519:1540	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	12	57	theme	unidentified	1547:1558	arg1	glycolipids					1560:1570	unidentified glycolipids	1547:1570	unidentified glycolipids	1547:1570	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	11	58	theme	G+C	1325:1327	arg1	content					1329:1335	The whole-genome DNA G+C content	1304:1335	The whole-genome DNA G+C content of strain DA-C8T	1304:1352	The whole-genome DNA G+C content of strain DA-C8T was 52.3 mol%.					
36943336	11	58	theme	G+C	1325:1327	arg1	%					1366:1366	52.3 mol%	1358:1366	52.3 mol%	1358:1366	The whole-genome DNA G+C content of strain DA-C8T was 52.3 mol%.					
36943336	9	59	theme	nucleotide	1053:1062	arg1	identity					1064:1071	average nucleotide identity	1045:1071	average nucleotide identity	1045:1071	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	9	59	theme	nucleotide	1053:1062	arg1	hybridization					1030:1042	Digital DNA-DNA hybridization	1014:1042	Digital DNA-DNA hybridization	1014:1042	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	12	60	theme	fatty	1388:1392	arg1	C16 					1405:1408	C16 	1405:1408	C16 	1405:1408	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	12	60	theme	fatty	1388:1392	arg1	acids					1394:1398	The major cellular fatty acids	1369:1398	The major cellular fatty acids	1369:1398	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	13	61	theme	species	1707:1713	arg1	sp					1747:1748	the novel species Insulambacter thermoxylanivorax sp	1697:1748	proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp	1660:1748	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	12	62	theme	polar	1529:1533	arg1	lipids					1535:1540	the major polar lipids	1519:1540	the major polar lipids	1519:1540	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	12	62	theme	polar	1529:1533	arg1	glycolipids					1560:1570	unidentified glycolipids	1547:1570	unidentified glycolipids	1547:1570	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	10	63	theme	composti	1249:1256	arg1	K13					1258:1260	X. composti K13	1246:1260	X. composti K13	1246:1260	Those between strain DA-C8T and X. composti K13 were 23.7, 67.7 and 57.6 %, respectively.					
36943336	2	64	theme	strain	220:225	arg1	DA-C8T					227:232	strain DA-C8T	220:232	strain DA-C8T	220:232	We isolated and analysed a Gram-negative, facultatively thermophilic, xylan-degrading bacterium that we designated as strain DA-C8T.					
36943336	15	65	theme	34211T=DSM	1788:1797	arg1	111723T					1799:1805	=JCM 34211T=DSM 111723T	1783:1805	=JCM 34211T=DSM 111723T	1783:1805	The type strain is DA-C8T (=JCM 34211T=DSM 111723T).					
36943336	15	65	theme	34211T=DSM	1788:1797	arg1	DA-C8T					1775:1780	DA-C8T	1775:1780	DA-C8T (=JCM 34211T=DSM 111723T)	1775:1806	The type strain is DA-C8T (=JCM 34211T=DSM 111723T).					
36943336	13	66	theme	Insulambacter	1715:1727	arg1	sp					1747:1748	the novel species Insulambacter thermoxylanivorax sp	1697:1748	proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp	1660:1748	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	15	67	theme	=JCM	1783:1786	arg1	111723T					1799:1805	=JCM 34211T=DSM 111723T	1783:1805	=JCM 34211T=DSM 111723T	1783:1805	The type strain is DA-C8T (=JCM 34211T=DSM 111723T).					
36943336	15	67	theme	=JCM	1783:1786	arg1	DA-C8T					1775:1780	DA-C8T	1775:1780	DA-C8T (=JCM 34211T=DSM 111723T)	1775:1806	The type strain is DA-C8T (=JCM 34211T=DSM 111723T).					
36943336	4	68	theme	growth	440:445	arg1	conditions					447:456	anaerobic growth conditions	430:456	anaerobic growth conditions	430:456	The strain showed high xylan degradation ability under anaerobic growth conditions.					
36943336	13	69	dep	nov.-for	1688:1695	arg1	sp					1747:1748	the novel species Insulambacter thermoxylanivorax sp	1697:1748	proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp	1660:1748	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	5	70	dep	37-60 °C	479:486	arg1	55 °C					498:502	55 °C	498:502	55 °C	498:502	The isolate grew at 37-60 °C (optimum, 55 °C) and pH 4.0-11.0 (optimum, pH 9.0).					
36943336	5	70	dep	37-60 °C	479:486	arg1	optimum					489:495	optimum	489:495	optimum	489:495	The isolate grew at 37-60 °C (optimum, 55 °C) and pH 4.0-11.0 (optimum, pH 9.0).					
36943336	9	71	theme	Digital	1014:1020	arg1	identity					1064:1071	average nucleotide identity	1045:1071	average nucleotide identity	1045:1071	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	9	71	theme	Digital	1014:1020	arg1	hybridization					1030:1042	Digital DNA-DNA hybridization	1014:1042	Digital DNA-DNA hybridization	1014:1042	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	13	72	theme	thermoxylanivorax	1729:1745	arg1	sp					1747:1748	the novel species Insulambacter thermoxylanivorax sp	1697:1748	proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp	1660:1748	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	7	73	theme	Paenibacillus	795:807	arg1	HSCC596					824:830	Paenibacillus chitinolyticus HSCC596	795:830	Paenibacillus chitinolyticus HSCC596 (93.5 %)	795:839	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	7	73	theme	Paenibacillus	795:807	arg1	%					838:838	93.5 %	833:838	93.5 %	833:838	Comparison of 16S rRNA gene sequences indicated that strain DA-C8T was most closely related to Paenibacillus cisolokensis LC2-13AT (93.9 %) and Paenibacillus chitinolyticus HSCC596 (93.5 %).					
36943336	3	74	theme	wood	337:340	arg1	source					367:372	the sole carbon source	351:372	the sole carbon source	351:372	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
36943336	3	74	theme	wood	337:340	arg1	xylan					342:346	beech wood xylan	331:346	beech wood xylan	331:346	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
36943336	4	75	theme	anaerobic	430:438	arg1	conditions					447:456	anaerobic growth conditions	430:456	anaerobic growth conditions	430:456	The strain showed high xylan degradation ability under anaerobic growth conditions.					
36943336	13	76	theme	gen.	1683:1686	arg1	genus					1651:1655	a novel genus	1643:1655	a novel genus	1643:1655	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	13	76	theme	gen.	1683:1686	arg1	nov.-for					1688:1695	proposed-Insulambacter gen. nov.-for	1660:1695	proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp	1660:1748	On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed-Insulambacter gen. nov.-for the novel species Insulambacter thermoxylanivorax sp.					
36943336	2	77	theme	Gram-negative	129:141	arg1	bacterium					188:196	a Gram-negative, facultatively thermophilic, xylan-degrading bacterium	127:196	bacterium	188:196	We isolated and analysed a Gram-negative, facultatively thermophilic, xylan-degrading bacterium that we designated as strain DA-C8T.					
36943336	1	78	theme	thermophilic	44:55	arg1	bacterium					69:77	a thermophilic xylanolytic bacterium	42:77	a thermophilic xylanolytic bacterium	42:77	nov., a thermophilic xylanolytic bacterium isolated from compost.					
36943336	1	78	theme	thermophilic	44:55	arg1	nov.					36:39	nov.	36:39	nov.	36:39	nov., a thermophilic xylanolytic bacterium isolated from compost.					
36943336	9	79	theme	average	1084:1090	arg1	identity					1112:1119	average amino acid sequence identity	1084:1119	average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT	1084:1170	Digital DNA-DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8T and P. cisolokensis LC2-13AT were 21.8, 68.3 and 58.2 %, respectively.					
36943336	6	80	theme	strain	558:563	arg1	DA-C8T					565:570	strain DA-C8T	558:570	strain DA-C8T	558:570	As well as xylan, strain DA-C8T could use polysaccharides such as arabinoxylan and galactan as carbon sources.					
36943336	12	81	theme	major	1482:1486	arg1	quinone					1488:1494	the major quinone	1478:1494	the major quinone	1478:1494	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	12	81	theme	major	1482:1486	arg1	menaquinone					1500:1510	menaquinone 7	1500:1512	menaquinone 7	1500:1512	The major cellular fatty acids were C16 : 0 (42.9 %), anteiso-C15 : 0 (20.0 %) and anteiso-C17 : 0 (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids.					
36943336	3	82	attach	isolated	250:257	arg1	Japan					294:298	Japan	294:298	Japan	294:298	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
36943336	3	82	attach	isolated	250:257	arg1	compost					264:270	compost	264:270	compost	264:270	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
36943336	3	82	attach	isolated	250:257	arg2	strain					239:244	The strain	235:244	The strain	235:244	The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source.					
35568899	12	0	theme	α-helix	1966:1972	arg1	regions					1974:1980	the N-terminal and α-helix regions	1947:1980	the N-terminal and α-helix regions	1947:1980	Thermostability of Xyl40 was most likely contributed by a disulphide bond and salt bridge in the N-terminal and α-helix regions.					
35568899	3	1	theme	family	498:503	arg1	xylanases					508:516	four glycoside hydrolase (GH) family 11 xylanases	468:516	four glycoside hydrolase (GH) family 11 xylanases	468:516	The effect of enzymatically isolated lignin on the hydrolysis of xylan by four glycoside hydrolase (GH) family 11 xylanases was studied.					
35568899	12	2	theme	salt	1932:1935	arg1	bridge					1937:1942	a disulphide bond and salt bridge	1910:1942	bridge	1937:1942	Thermostability of Xyl40 was most likely contributed by a disulphide bond and salt bridge in the N-terminal and α-helix regions.					
35568899	9	3	theme	thermal	1578:1584	arg1	stability					1586:1594	the increased thermal stability	1564:1594	the increased thermal stability of Xyl40	1564:1603	By comparing the 3D structures of these xylanases, features contributing to the increased thermal stability of Xyl40 were identified.					
35568899	7	4	with	incubation	1081:1090	arg1	lignin					1114:1119	lignin	1114:1119	lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin	1114:1186	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	2	5	theme	pretreated	275:284	arg1	biomasses					286:294	steam pretreated biomasses	269:294	steam pretreated biomasses	269:294	Lignin from steam pretreated biomasses is known to inhibit enzymes by non-productively binding enzymes and limiting access to cellulose.					
35568899	6	6	theme	thermostable	863:874	arg1	TrXyn1					885:890	the least thermostable xylanase TrXyn1	853:890	the least thermostable xylanase TrXyn1	853:890	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	4	7	theme	metagenomic	633:643	arg1	Xyl40					654:658	a thermostable metagenomic xylanase Xyl40	618:658	a thermostable metagenomic xylanase Xyl40	618:658	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2, and two forms of a thermostable metagenomic xylanase Xyl40 were compared.					
35568899	4	8	theme	Xyl40	654:658	arg1	forms					609:613	two forms	605:613	two forms of a thermostable metagenomic xylanase Xyl40	605:658	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2, and two forms of a thermostable metagenomic xylanase Xyl40 were compared.					
35568899	4	8	theme	Xyl40	654:658	arg1	xylanases					535:543	Two xylanases	531:543	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2,	531:599	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2, and two forms of a thermostable metagenomic xylanase Xyl40 were compared.					
35568899	7	9	theme	reesei	1306:1311	arg1	xylanases					1313:1321	the mesophilic T. reesei xylanases	1288:1321	the mesophilic T. reesei xylanases	1288:1321	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	7	10	theme	Enzyme	1024:1029	arg1	activity					1031:1038	Enzyme activity	1024:1038	Enzyme activity	1024:1038	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	7	11	theme	mesophilic	1292:1301	arg1	xylanases					1313:1321	the mesophilic T. reesei xylanases	1288:1321	the mesophilic T. reesei xylanases	1288:1321	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	5	12	theme	steam	704:708	arg1	spruce					721:726	steam pretreated spruce	704:726	steam pretreated spruce	704:726	RESULTS Lignin isolated from steam pretreated spruce decreased the hydrolysis yields of xylan for all the xylanases at 40 and 50 °C.					
35568899	8	13	theme	catalytic	1371:1379	arg1	Xyl40					1391:1395	Xyl40	1391:1395	Xyl40	1391:1395	Removing of N-glycans from the catalytic domain of Xyl40 increased lignin inhibition in hydrolysis of xylan when compared to the glycosylated form.					
35568899	8	13	theme	catalytic	1371:1379	arg1	domain					1381:1386	the catalytic domain	1367:1386	the catalytic domain of Xyl40	1367:1395	Removing of N-glycans from the catalytic domain of Xyl40 increased lignin inhibition in hydrolysis of xylan when compared to the glycosylated form.					
35568899	1	14	theme	Enzymatic	115:123	arg1	hydrolysis					125:134	BACKGROUND Enzymatic hydrolysis	104:134	BACKGROUND Enzymatic hydrolysis of lignocellulosic biomass into platform sugars	104:182	BACKGROUND Enzymatic hydrolysis of lignocellulosic biomass into platform sugars can be enhanced by the addition of accessory enzymes, such as xylanases.					
35568899	2	15	from	biomasses	286:294	arg1	Lignin					257:262	Lignin	257:262	Lignin from steam pretreated biomasses	257:294	Lignin from steam pretreated biomasses is known to inhibit enzymes by non-productively binding enzymes and limiting access to cellulose.					
35568899	7	16	from	xylanases	1162:1170	arg1	at					1136:1137	up to 24 h at 40 °C. All the studied xylanases	1125:1170	up to 24 h at 40 °C. All the studied xylanases bound to lignin	1125:1186	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	0	17	theme	xylan	49:53	arg1	hydrolysis					35:44	the hydrolysis	31:44	the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanases	31:101	Inhibitory effect of lignin on the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanases.					
35568899	3	18	from	effect	398:403	arg1	hydrolysis					445:454	the hydrolysis	441:454	the hydrolysis of xylan by four glycoside hydrolase (GH) family 11 xylanases	441:516	The effect of enzymatically isolated lignin on the hydrolysis of xylan by four glycoside hydrolase (GH) family 11 xylanases was studied.					
35568899	1	19	theme	biomass	155:161	arg1	hydrolysis					125:134	BACKGROUND Enzymatic hydrolysis	104:134	BACKGROUND Enzymatic hydrolysis of lignocellulosic biomass into platform sugars	104:182	BACKGROUND Enzymatic hydrolysis of lignocellulosic biomass into platform sugars can be enhanced by the addition of accessory enzymes, such as xylanases.					
35568899	0	20	theme	thermophilic	58:69	arg1	xylanases					93:101	thermophilic and thermolabile GH11 xylanases	58:101	thermophilic and thermolabile GH11 xylanases	58:101	Inhibitory effect of lignin on the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanases.					
35568899	1	21	theme	platform	168:175	arg1	sugars					177:182	platform sugars	168:182	platform sugars	168:182	BACKGROUND Enzymatic hydrolysis of lignocellulosic biomass into platform sugars can be enhanced by the addition of accessory enzymes, such as xylanases.					
35568899	0	22	theme	thermolabile	75:86	arg1	xylanases					93:101	thermophilic and thermolabile GH11 xylanases	58:101	thermophilic and thermolabile GH11 xylanases	58:101	Inhibitory effect of lignin on the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanases.					
35568899	8	23	gly	glycosylated	1469:1480	arg1	form					1482:1485	the glycosylated form	1465:1485	the glycosylated form	1465:1485	Removing of N-glycans from the catalytic domain of Xyl40 increased lignin inhibition in hydrolysis of xylan when compared to the glycosylated form.					
35568899	8	24	theme	xylan	1442:1446	arg1	hydrolysis					1428:1437	hydrolysis	1428:1437	hydrolysis of xylan	1428:1446	Removing of N-glycans from the catalytic domain of Xyl40 increased lignin inhibition in hydrolysis of xylan when compared to the glycosylated form.					
35568899	7	25	theme	lignin	1255:1260	arg1	surface					1262:1268	the lignin surface	1251:1268	the lignin surface for 24 h	1251:1277	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	5	26	theme	hydrolysis	742:751	arg1	yields					753:758	the hydrolysis yields	738:758	the hydrolysis yields of xylan for all the xylanases at 40 and 50 °C	738:805	RESULTS Lignin isolated from steam pretreated spruce decreased the hydrolysis yields of xylan for all the xylanases at 40 and 50 °C.					
35568899	12	27	from	bridge	1937:1942	arg1	regions					1974:1980	the N-terminal and α-helix regions	1947:1980	the N-terminal and α-helix regions	1947:1980	Thermostability of Xyl40 was most likely contributed by a disulphide bond and salt bridge in the N-terminal and α-helix regions.					
35568899	3	28	theme	lignin	431:436	arg1	effect					398:403	The effect	394:403	The effect of enzymatically isolated lignin on the hydrolysis of xylan by four glycoside hydrolase (GH) family 11 xylanases	394:516	The effect of enzymatically isolated lignin on the hydrolysis of xylan by four glycoside hydrolase (GH) family 11 xylanases was studied.					
35568899	0	29	theme	Inhibitory	0:9	arg1	effect					11:16	Inhibitory effect	0:16	Inhibitory effect of lignin on the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanases	0:101	Inhibitory effect of lignin on the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanases.					
35568899	5	30	theme	pretreated	710:719	arg1	spruce					721:726	steam pretreated spruce	704:726	steam pretreated spruce	704:726	RESULTS Lignin isolated from steam pretreated spruce decreased the hydrolysis yields of xylan for all the xylanases at 40 and 50 °C.					
35568899	12	31	from	bond	1923:1926	arg1	regions					1974:1980	the N-terminal and α-helix regions	1947:1980	the N-terminal and α-helix regions	1947:1980	Thermostability of Xyl40 was most likely contributed by a disulphide bond and salt bridge in the N-terminal and α-helix regions.					
35568899	9	32	theme	3D	1505:1506	arg1	structures					1508:1517	the 3D structures	1501:1517	the 3D structures of these xylanases	1501:1536	By comparing the 3D structures of these xylanases, features contributing to the increased thermal stability of Xyl40 were identified.					
35568899	7	33	theme	xylanases	1099:1107	arg1	incubation					1081:1090	incubation	1081:1090	incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin	1081:1186	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	11	34	theme	domain	1792:1797	arg1	N-glycosylation					1759:1773	N-glycosylation	1759:1773	N-glycosylation of the catalytic domain of Xyl40	1759:1806	N-glycosylation of the catalytic domain of Xyl40 increased the lignin tolerance of the enzyme.					
35568899	6	35	theme	hydrolysis	820:829	arg1	temperature					831:841	elevated hydrolysis temperature	811:841	elevated hydrolysis temperature of 50 °C	811:850	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	9	36	theme	xylanases	1528:1536	arg1	structures					1508:1517	the 3D structures	1501:1517	the 3D structures of these xylanases	1501:1536	By comparing the 3D structures of these xylanases, features contributing to the increased thermal stability of Xyl40 were identified.					
35568899	10	37	theme	CONCLUSIONS	1622:1632	arg1	stability					1647:1655	CONCLUSIONS High thermal stability	1622:1655	CONCLUSIONS High thermal stability of xylanases Xyl40 and Xyl40-CD	1622:1687	CONCLUSIONS High thermal stability of xylanases Xyl40 and Xyl40-CD enabled the enzymes to remain partially active on the lignin surface.					
35568899	1	38	theme	accessory	219:227	arg1	xylanases					246:254	xylanases	246:254	xylanases	246:254	BACKGROUND Enzymatic hydrolysis of lignocellulosic biomass into platform sugars can be enhanced by the addition of accessory enzymes, such as xylanases.					
35568899	1	38	theme	accessory	219:227	arg1	enzymes					229:235	accessory enzymes	219:235	accessory enzymes	219:235	BACKGROUND Enzymatic hydrolysis of lignocellulosic biomass into platform sugars can be enhanced by the addition of accessory enzymes, such as xylanases.					
35568899	11	39	theme	Xyl40	1802:1806	arg1	domain					1792:1797	the catalytic domain	1778:1797	the catalytic domain of Xyl40	1778:1806	N-glycosylation of the catalytic domain of Xyl40 increased the lignin tolerance of the enzyme.					
35568899	11	39	theme	Xyl40	1802:1806	arg1	Xyl40					1802:1806	Xyl40	1802:1806	Xyl40	1802:1806	N-glycosylation of the catalytic domain of Xyl40 increased the lignin tolerance of the enzyme.					
35568899	9	40	theme	increased	1568:1576	arg1	stability					1586:1594	the increased thermal stability	1564:1594	the increased thermal stability of Xyl40	1564:1603	By comparing the 3D structures of these xylanases, features contributing to the increased thermal stability of Xyl40 were identified.					
35568899	4	41	theme	Trichoderma	565:575	arg1	TrXyn2					593:598	TrXyn2	593:598	TrXyn2	593:598	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2, and two forms of a thermostable metagenomic xylanase Xyl40 were compared.					
35568899	4	41	theme	Trichoderma	565:575	arg1	reesei					577:582	the mesophilic Trichoderma reesei	550:582	the mesophilic Trichoderma reesei	550:582	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2, and two forms of a thermostable metagenomic xylanase Xyl40 were compared.					
35568899	6	42	theme	xylanase	876:883	arg1	TrXyn1					885:890	the least thermostable xylanase TrXyn1	853:890	the least thermostable xylanase TrXyn1	853:890	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	10	43	theme	thermal	1639:1645	arg1	stability					1647:1655	CONCLUSIONS High thermal stability	1622:1655	CONCLUSIONS High thermal stability of xylanases Xyl40 and Xyl40-CD	1622:1687	CONCLUSIONS High thermal stability of xylanases Xyl40 and Xyl40-CD enabled the enzymes to remain partially active on the lignin surface.					
35568899	2	44	theme	steam	269:273	arg1	biomasses					286:294	steam pretreated biomasses	269:294	steam pretreated biomasses	269:294	Lignin from steam pretreated biomasses is known to inhibit enzymes by non-productively binding enzymes and limiting access to cellulose.					
35568899	9	45	theme	Xyl40	1599:1603	arg1	stability					1586:1594	the increased thermal stability	1564:1594	the increased thermal stability of Xyl40	1564:1603	By comparing the 3D structures of these xylanases, features contributing to the increased thermal stability of Xyl40 were identified.					
35568899	4	46	theme	thermostable	620:631	arg1	Xyl40					654:658	a thermostable metagenomic xylanase Xyl40	618:658	a thermostable metagenomic xylanase Xyl40	618:658	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2, and two forms of a thermostable metagenomic xylanase Xyl40 were compared.					
35568899	5	47	theme	xylan	763:767	arg1	yields					753:758	the hydrolysis yields	738:758	the hydrolysis yields of xylan for all the xylanases at 40 and 50 °C	738:805	RESULTS Lignin isolated from steam pretreated spruce decreased the hydrolysis yields of xylan for all the xylanases at 40 and 50 °C.					
35568899	4	48	theme	xylanase	645:652	arg1	Xyl40					654:658	a thermostable metagenomic xylanase Xyl40	618:658	a thermostable metagenomic xylanase Xyl40	618:658	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2, and two forms of a thermostable metagenomic xylanase Xyl40 were compared.					
35568899	12	49	theme	disulphide	1912:1921	arg1	bond					1923:1926	a disulphide bond and salt bridge	1910:1942	bond	1923:1926	Thermostability of Xyl40 was most likely contributed by a disulphide bond and salt bridge in the N-terminal and α-helix regions.					
35568899	6	50	theme	thermostable	934:945	arg1	domain					971:976	the catalytic domain	957:976	the catalytic domain (CD) of Xyl40	957:990	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	6	50	theme	thermostable	934:945	arg1	xylanase					947:954	the most thermostable xylanase	925:954	the most thermostable xylanase	925:954	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	6	50	theme	thermostable	934:945	arg1	Xyl40					986:990	Xyl40	986:990	Xyl40	986:990	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	3	51	theme	GH	494:495	arg1	xylanases					508:516	four glycoside hydrolase (GH) family 11 xylanases	468:516	four glycoside hydrolase (GH) family 11 xylanases	468:516	The effect of enzymatically isolated lignin on the hydrolysis of xylan by four glycoside hydrolase (GH) family 11 xylanases was studied.					
35568899	10	52	theme	lignin	1743:1748	arg1	surface					1750:1756	the lignin surface	1739:1756	the lignin surface	1739:1756	CONCLUSIONS High thermal stability of xylanases Xyl40 and Xyl40-CD enabled the enzymes to remain partially active on the lignin surface.					
35568899	7	53	theme	at	1136:1137	arg1	xylanases					1162:1170	up to 24 h at 40 °C. All the studied xylanases	1125:1170	up to 24 h at 40 °C. All the studied xylanases bound to lignin	1125:1186	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	1	54	theme	BACKGROUND	104:113	arg1	hydrolysis					125:134	BACKGROUND Enzymatic hydrolysis	104:134	BACKGROUND Enzymatic hydrolysis of lignocellulosic biomass into platform sugars	104:182	BACKGROUND Enzymatic hydrolysis of lignocellulosic biomass into platform sugars can be enhanced by the addition of accessory enzymes, such as xylanases.					
35568899	10	55	theme	xylanases	1660:1668	arg1	stability					1647:1655	CONCLUSIONS High thermal stability	1622:1655	CONCLUSIONS High thermal stability of xylanases Xyl40 and Xyl40-CD	1622:1687	CONCLUSIONS High thermal stability of xylanases Xyl40 and Xyl40-CD enabled the enzymes to remain partially active on the lignin surface.					
35568899	8	56	theme	Xyl40	1391:1395	arg1	Xyl40					1391:1395	Xyl40	1391:1395	Xyl40	1391:1395	Removing of N-glycans from the catalytic domain of Xyl40 increased lignin inhibition in hydrolysis of xylan when compared to the glycosylated form.					
35568899	8	56	theme	Xyl40	1391:1395	arg1	domain					1381:1386	the catalytic domain	1367:1386	the catalytic domain of Xyl40	1367:1395	Removing of N-glycans from the catalytic domain of Xyl40 increased lignin inhibition in hydrolysis of xylan when compared to the glycosylated form.					
35568899	6	57	theme	Xyl40	986:990	arg1	domain					971:976	the catalytic domain	957:976	the catalytic domain (CD) of Xyl40	957:990	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	6	57	theme	Xyl40	986:990	arg1	xylanase					947:954	the most thermostable xylanase	925:954	the most thermostable xylanase	925:954	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	6	57	theme	Xyl40	986:990	arg1	CD					979:980	CD	979:980	CD	979:980	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	6	57	theme	Xyl40	986:990	arg1	Xyl40					986:990	Xyl40	986:990	Xyl40	986:990	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	8	58	theme	lignin	1407:1412	arg1	inhibition					1414:1423	lignin inhibition	1407:1423	lignin inhibition	1407:1423	Removing of N-glycans from the catalytic domain of Xyl40 increased lignin inhibition in hydrolysis of xylan when compared to the glycosylated form.					
35568899	3	59	theme	glycoside	473:481	arg1	xylanases					508:516	four glycoside hydrolase (GH) family 11 xylanases	468:516	four glycoside hydrolase (GH) family 11 xylanases	468:516	The effect of enzymatically isolated lignin on the hydrolysis of xylan by four glycoside hydrolase (GH) family 11 xylanases was studied.					
35568899	7	60	theme	studied	1154:1160	arg1	xylanases					1162:1170	up to 24 h at 40 °C. All the studied xylanases	1125:1170	up to 24 h at 40 °C. All the studied xylanases bound to lignin	1125:1186	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	1	61	theme	lignocellulosic	139:153	arg1	biomass					155:161	lignocellulosic biomass	139:161	lignocellulosic biomass	139:161	BACKGROUND Enzymatic hydrolysis of lignocellulosic biomass into platform sugars can be enhanced by the addition of accessory enzymes, such as xylanases.					
35568899	0	62	from	effect	11:16	arg1	hydrolysis					35:44	the hydrolysis	31:44	the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanases	31:101	Inhibitory effect of lignin on the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanases.					
35568899	0	63	theme	GH11	88:91	arg1	xylanases					93:101	thermophilic and thermolabile GH11 xylanases	58:101	thermophilic and thermolabile GH11 xylanases	58:101	Inhibitory effect of lignin on the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanases.					
35568899	12	64	theme	Xyl40	1873:1877	arg1	Thermostability					1854:1868	Thermostability	1854:1868	Thermostability of Xyl40	1854:1877	Thermostability of Xyl40 was most likely contributed by a disulphide bond and salt bridge in the N-terminal and α-helix regions.					
35568899	6	65	theme	catalytic	961:969	arg1	domain					971:976	the catalytic domain	957:976	the catalytic domain (CD) of Xyl40	957:990	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	6	65	theme	catalytic	961:969	arg1	xylanase					947:954	the most thermostable xylanase	925:954	the most thermostable xylanase	925:954	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	6	65	theme	catalytic	961:969	arg1	CD					979:980	CD	979:980	CD	979:980	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	6	65	theme	catalytic	961:969	arg1	Xyl40					986:990	Xyl40	986:990	Xyl40	986:990	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	5	66	dep	RESULTS	675:681	arg1	decreased					728:736	decreased	728:736	decreased the hydrolysis yields of xylan for all the xylanases at 40 and 50 °C	728:805	RESULTS Lignin isolated from steam pretreated spruce decreased the hydrolysis yields of xylan for all the xylanases at 40 and 50 °C.					
35568899	5	67	attach	isolated	690:697	arg2	Lignin					683:688	Lignin	683:688	Lignin isolated from steam pretreated spruce	683:726	RESULTS Lignin isolated from steam pretreated spruce decreased the hydrolysis yields of xylan for all the xylanases at 40 and 50 °C.					
35568899	5	67	attach	isolated	690:697	arg1	spruce					721:726	steam pretreated spruce	704:726	steam pretreated spruce	704:726	RESULTS Lignin isolated from steam pretreated spruce decreased the hydrolysis yields of xylan for all the xylanases at 40 and 50 °C.					
35568899	3	68	theme	hydrolase	483:491	arg1	xylanases					508:516	four glycoside hydrolase (GH) family 11 xylanases	468:516	four glycoside hydrolase (GH) family 11 xylanases	468:516	The effect of enzymatically isolated lignin on the hydrolysis of xylan by four glycoside hydrolase (GH) family 11 xylanases was studied.					
35568899	4	69	from	reesei	577:582	arg1	forms					609:613	two forms	605:613	two forms of a thermostable metagenomic xylanase Xyl40	605:658	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2, and two forms of a thermostable metagenomic xylanase Xyl40 were compared.					
35568899	4	69	from	reesei	577:582	arg1	xylanases					535:543	Two xylanases	531:543	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2,	531:599	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2, and two forms of a thermostable metagenomic xylanase Xyl40 were compared.					
35568899	5	70	from	50 °C	801:805	arg1	xylanases					781:789	all the xylanases	773:789	all the xylanases at 40 and 50 °C	773:805	RESULTS Lignin isolated from steam pretreated spruce decreased the hydrolysis yields of xylan for all the xylanases at 40 and 50 °C.					
35568899	10	71	dep	xylanases	1660:1668	arg1	xylanases					1660:1668	xylanases Xyl40 and Xyl40-CD	1660:1687	xylanases Xyl40 and Xyl40-CD	1660:1687	CONCLUSIONS High thermal stability of xylanases Xyl40 and Xyl40-CD enabled the enzymes to remain partially active on the lignin surface.					
35568899	10	71	dep	xylanases	1660:1668	arg1	Xyl40-CD					1680:1687	Xyl40-CD	1680:1687	Xyl40-CD	1680:1687	CONCLUSIONS High thermal stability of xylanases Xyl40 and Xyl40-CD enabled the enzymes to remain partially active on the lignin surface.					
35568899	10	71	dep	xylanases	1660:1668	arg1	Xyl40					1670:1674	Xyl40	1670:1674	Xyl40	1670:1674	CONCLUSIONS High thermal stability of xylanases Xyl40 and Xyl40-CD enabled the enzymes to remain partially active on the lignin surface.					
35568899	11	72	theme	enzyme	1846:1851	arg1	tolerance					1829:1837	the lignin tolerance	1818:1837	the lignin tolerance of the enzyme	1818:1851	N-glycosylation of the catalytic domain of Xyl40 increased the lignin tolerance of the enzyme.					
35568899	5	73	from	40	794:795	arg1	xylanases					781:789	all the xylanases	773:789	all the xylanases at 40 and 50 °C	773:805	RESULTS Lignin isolated from steam pretreated spruce decreased the hydrolysis yields of xylan for all the xylanases at 40 and 50 °C.					
35568899	7	74	theme	activity	1239:1246	arg1	%					1234:1234	22-39%	1229:1234	22-39% of activity	1229:1246	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	7	74	theme	activity	1239:1246	arg1	activity					1239:1246	activity	1239:1246	activity	1239:1246	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	8	75	theme	glycosylated	1469:1480	arg1	form					1482:1485	the glycosylated form	1465:1485	the glycosylated form	1465:1485	Removing of N-glycans from the catalytic domain of Xyl40 increased lignin inhibition in hydrolysis of xylan when compared to the glycosylated form.					
35568899	0	76	theme	lignin	21:26	arg1	effect					11:16	Inhibitory effect	0:16	Inhibitory effect of lignin on the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanases	0:101	Inhibitory effect of lignin on the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanases.					
35568899	4	77	theme	mesophilic	554:563	arg1	TrXyn2					593:598	TrXyn2	593:598	TrXyn2	593:598	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2, and two forms of a thermostable metagenomic xylanase Xyl40 were compared.					
35568899	4	77	theme	mesophilic	554:563	arg1	reesei					577:582	the mesophilic Trichoderma reesei	550:582	the mesophilic Trichoderma reesei	550:582	Two xylanases from the mesophilic Trichoderma reesei, TrXyn1, TrXyn2, and two forms of a thermostable metagenomic xylanase Xyl40 were compared.					
35568899	6	78	theme	50 °C	846:850	arg1	temperature					831:841	elevated hydrolysis temperature	811:841	elevated hydrolysis temperature of 50 °C	811:850	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	3	79	theme	isolated	422:429	arg1	lignin					431:436	enzymatically isolated lignin	408:436	enzymatically isolated lignin	408:436	The effect of enzymatically isolated lignin on the hydrolysis of xylan by four glycoside hydrolase (GH) family 11 xylanases was studied.					
35568899	11	80	gly	N-glycosylation	1759:1773	arg1	domain					1792:1797	the catalytic domain	1778:1797	the catalytic domain of Xyl40	1778:1806	N-glycosylation of the catalytic domain of Xyl40 increased the lignin tolerance of the enzyme.					
35568899	11	80	gly	N-glycosylation	1759:1773	arg1	Xyl40					1802:1806	Xyl40	1802:1806	Xyl40	1802:1806	N-glycosylation of the catalytic domain of Xyl40 increased the lignin tolerance of the enzyme.					
35568899	3	81	theme	xylan	459:463	arg1	hydrolysis					445:454	the hydrolysis	441:454	the hydrolysis of xylan by four glycoside hydrolase (GH) family 11 xylanases	441:516	The effect of enzymatically isolated lignin on the hydrolysis of xylan by four glycoside hydrolase (GH) family 11 xylanases was studied.					
35568899	7	82	theme	thermostable	1197:1208	arg1	xylanases					1210:1218	the thermostable xylanases	1193:1218	the thermostable xylanases	1193:1218	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	7	83	dep	24 h	1131:1134	arg1	to					1128:1129	to	1128:1129	to	1128:1129	Enzyme activity and binding to lignin were studied after incubation of the xylanases with lignin for up to 24 h at 40 °C. All the studied xylanases bound to lignin, but the thermostable xylanases retained 22-39% of activity on the lignin surface for 24 h, whereas the mesophilic T. reesei xylanases become inactive.					
35568899	10	84	theme	High	1634:1637	arg1	stability					1647:1655	CONCLUSIONS High thermal stability	1622:1655	CONCLUSIONS High thermal stability of xylanases Xyl40 and Xyl40-CD	1622:1687	CONCLUSIONS High thermal stability of xylanases Xyl40 and Xyl40-CD enabled the enzymes to remain partially active on the lignin surface.					
35568899	6	85	theme	elevated	811:818	arg1	temperature					831:841	elevated hydrolysis temperature	811:841	elevated hydrolysis temperature of 50 °C	811:850	At elevated hydrolysis temperature of 50 °C, the least thermostable xylanase TrXyn1 was most inhibited by lignin and the most thermostable xylanase, the catalytic domain (CD) of Xyl40, was least inhibited by lignin.					
35568899	11	86	theme	catalytic	1782:1790	arg1	domain					1792:1797	the catalytic domain	1778:1797	the catalytic domain of Xyl40	1778:1806	N-glycosylation of the catalytic domain of Xyl40 increased the lignin tolerance of the enzyme.					
35568899	11	86	theme	catalytic	1782:1790	arg1	Xyl40					1802:1806	Xyl40	1802:1806	Xyl40	1802:1806	N-glycosylation of the catalytic domain of Xyl40 increased the lignin tolerance of the enzyme.					
35568899	1	87	theme	enzymes	229:235	arg1	addition					207:214	the addition	203:214	the addition of accessory enzymes, such as xylanases	203:254	BACKGROUND Enzymatic hydrolysis of lignocellulosic biomass into platform sugars can be enhanced by the addition of accessory enzymes, such as xylanases.					
35568899	12	88	theme	N-terminal	1951:1960	arg1	regions					1974:1980	the N-terminal and α-helix regions	1947:1980	the N-terminal and α-helix regions	1947:1980	Thermostability of Xyl40 was most likely contributed by a disulphide bond and salt bridge in the N-terminal and α-helix regions.					
35568899	11	89	theme	lignin	1822:1827	arg1	tolerance					1829:1837	the lignin tolerance	1818:1837	the lignin tolerance of the enzyme	1818:1851	N-glycosylation of the catalytic domain of Xyl40 increased the lignin tolerance of the enzyme.					
35316068	6	0	theme	mixture	873:879	arg1	point					851:855	The melting point	839:855	The melting point of the eutectic mixture	839:879	The melting point of the eutectic mixture was calculated according to the Schröder-van Laar equation.					
35316068	5	1	theme	Differential	619:630	arg1	calorimetry					641:651	Differential scanning calorimetry	619:651	Differential scanning calorimetry	619:651	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	14	2	theme	LAC	2141:2143	arg1	%					2136:2136	>60%	2133:2136	>60% of LAC for 28 days	2133:2155	The coamorphous samples containing 10-50% of LAC remained in an amorphous state for 21 days, and no crystallization was observed for the samples containing >60% of LAC for 28 days.					
35316068	14	2	theme	LAC	2141:2143	arg1	LAC					2141:2143	LAC	2141:2143	LAC	2141:2143	The coamorphous samples containing 10-50% of LAC remained in an amorphous state for 21 days, and no crystallization was observed for the samples containing >60% of LAC for 28 days.					
35316068	11	3	theme	hydroxyl	1678:1685	arg1	DPH					1666:1668	DPH	1666:1668	DPH	1666:1668	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	11	3	theme	hydroxyl	1678:1685	arg1	LAC					1696:1698	LAC	1696:1698	LAC	1696:1698	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	11	3	theme	hydroxyl	1678:1685	arg1	group					1687:1691	the hydroxyl group	1674:1691	the hydroxyl group of LAC	1674:1698	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	12	4	theme	DPH	1812:1814	arg1	ratio					1803:1807	the 70:30 molar ratio	1787:1807	the 70:30 molar ratio of DPH to LAC	1787:1821	Principal component analysis of the infrared spectra revealed a significant change at the 70:30 molar ratio of DPH to LAC, which was in agreement with the results of the thermal analysis.					
35316068	11	5	theme	LAC	1696:1698	arg1	DPH					1666:1668	DPH	1666:1668	DPH	1666:1668	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	11	5	theme	LAC	1696:1698	arg1	group					1657:1661	the amino group	1647:1661	the amino group of DPH	1647:1668	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	11	5	theme	LAC	1696:1698	arg1	group					1687:1691	the hydroxyl group	1674:1691	the hydroxyl group of LAC	1674:1698	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	11	5	theme	LAC	1696:1698	arg1	LAC					1696:1698	LAC	1696:1698	LAC	1696:1698	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	2	6	theme	transition	283:292	arg1	low					353:355	low	353:355	low	353:355	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	2	6	theme	transition	283:292	arg1	Tgs					308:310	Tgs	308:310	Tgs	308:310	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	2	6	theme	transition	283:292	arg1	temperatures					294:305	the glass transition temperatures	273:305	the glass transition temperatures (Tgs) of coamorphous materials	273:336	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	1	7	theme	small	187:191	arg1	molecules					193:201	small molecules	187:201	small molecules	187:201	Coamorphous systems comprising small molecules are emerging as counterparts to polymeric solid dispersions.					
35316068	7	8	theme	components	1081:1090	arg1	peaks					1063:1067	the melting peaks	1051:1067	the melting peaks of the pure components	1051:1090	The heat of fusion of the eutectic mixture was maximized at a 70:30 molar ratio of DPH to LAC; at this point, the melting peaks of the pure components disappeared.					
35316068	8	9	theme	DPH-LAC	1165:1171	arg1	mixtures					1182:1189	DPH-LAC physical mixtures	1165:1189	DPH-LAC physical mixtures at the ratios from 10:90 to 90:10	1165:1223	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	15	10	dep	crystallization	2269:2283	arg1	storage					2292:2298	storage	2292:2298	storage	2292:2298	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC might have caused crystallization during storage.					
35316068	2	11	theme	coamorphous	316:326	arg1	materials					328:336	coamorphous materials	316:336	coamorphous materials	316:336	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	8	12	theme	flow	1114:1117	arg1	profiles					1119:1126	The heat flow profiles	1105:1126	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10	1105:1223	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	8	13	theme	mixtures	1182:1189	arg1	cooling					1154:1160	cooling	1154:1160	cooling	1154:1160	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	8	13	theme	mixtures	1182:1189	arg1	melting					1142:1148	melting	1142:1148	melting	1142:1148	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	15	14	contain	containing	2226:2235	arg2	LAC					2247:2249	LAC	2247:2249	LAC	2247:2249	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC might have caused crystallization during storage.					
35316068	15	14	contain	containing	2226:2235	arg1	system					2219:2224	the coamorphous system	2203:2224	the coamorphous system containing 10-50% of LAC	2203:2249	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC might have caused crystallization during storage.					
35316068	15	14	contain	containing	2226:2235	arg2	%					2242:2242	10-50%	2237:2242	10-50% of LAC	2237:2249	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC might have caused crystallization during storage.					
35316068	13	15	theme	stability	1891:1899	arg1	test					1901:1904	A stability test	1889:1904	A stability test at 40 °C	1889:1913	A stability test at 40 °C revealed rapid crystallization of the supercooled liquid DPH.					
35316068	6	16	theme	Schröder-van	913:924	arg1	equation					931:938	the Schröder-van Laar equation	909:938	the Schröder-van Laar equation	909:938	The melting point of the eutectic mixture was calculated according to the Schröder-van Laar equation.					
35316068	0	17	theme	Glass	126:130	arg1	Temperature					143:153	a Low Glass Transition Temperature	120:153	a Low Glass Transition Temperature	120:153	Design of a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature.					
35316068	5	18	from	decrease	695:702	arg1	points					724:729	the neat melting points	707:729	the neat melting points depending on the composition of the physical mixtures	707:783	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	5	18	from	decrease	695:702	arg1	composition					748:758	the composition	744:758	the composition of the physical mixtures	744:783	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	7	19	theme	eutectic	967:974	arg1	mixture					976:982	the eutectic mixture	963:982	the eutectic mixture	963:982	The heat of fusion of the eutectic mixture was maximized at a 70:30 molar ratio of DPH to LAC; at this point, the melting peaks of the pure components disappeared.					
35316068	9	20	theme	DPH	1345:1347	arg1	Tg					1339:1340	the Tg	1335:1340	the Tg of DPH	1335:1347	Lactose showed a Tg of over 100 °C, and the Tg of DPH increased with the molar ratio of LAC; it was 84 °C at a 10:90 molar ratio of DPH to LAC.					
35316068	12	21	theme	significant	1765:1775	arg1	change					1777:1782	a significant change	1763:1782	a significant change	1763:1782	Principal component analysis of the infrared spectra revealed a significant change at the 70:30 molar ratio of DPH to LAC, which was in agreement with the results of the thermal analysis.					
35316068	11	22	from	shifts	1554:1559	arg1	spectra					1577:1583	the infrared spectra	1564:1583	the infrared spectra	1564:1583	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	12	23	theme	analysis	1879:1886	arg1	results					1856:1862	the results	1852:1862	the results of the thermal analysis	1852:1886	Principal component analysis of the infrared spectra revealed a significant change at the 70:30 molar ratio of DPH to LAC, which was in agreement with the results of the thermal analysis.					
35316068	11	24	theme	interactions	1626:1637	arg1	presence					1599:1606	the presence	1595:1606	the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC	1595:1698	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	8	25	from	cooling	1154:1160	arg1	ratios					1198:1203	the ratios	1194:1203	the ratios from 10:90 to 90:10	1194:1223	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	15	26	theme	system	2219:2224	arg1	°C					2196:2197	less than 40 °C	2183:2197	less than 40 °C	2183:2197	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC might have caused crystallization during storage.					
35316068	15	26	theme	system	2219:2224	arg1	Tg					2179:2180	The relatively lower Tg	2158:2180	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC	2158:2249	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC might have caused crystallization during storage.					
35316068	5	27	dep	decrease	695:702	arg1	addition					681:688	addition	681:688	addition	681:688	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	9	28	theme	molar	1368:1372	arg1	ratio					1374:1378	the molar ratio	1364:1378	the molar ratio of LAC	1364:1385	Lactose showed a Tg of over 100 °C, and the Tg of DPH increased with the molar ratio of LAC; it was 84 °C at a 10:90 molar ratio of DPH to LAC.					
35316068	3	29	theme	lactose	469:475	arg1	applicability					452:464	the applicability	448:464	the applicability of lactose (LAC) as an antiplasticizing coformer to a coamorphous system	448:537	This study aimed to investigate the applicability of lactose (LAC) as an antiplasticizing coformer to a coamorphous system.					
35316068	15	30	theme	lower	2173:2177	arg1	°C					2196:2197	less than 40 °C	2183:2197	less than 40 °C	2183:2197	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC might have caused crystallization during storage.					
35316068	15	30	theme	lower	2173:2177	arg1	Tg					2179:2180	The relatively lower Tg	2158:2180	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC	2158:2249	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC might have caused crystallization during storage.					
35316068	8	31	theme	single	1234:1239	arg1	Tg					1241:1242	a single Tg	1232:1242	a single Tg	1232:1242	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	0	32	theme	Antiplasticizing	58:73	arg1	Agent					75:79	an Antiplasticizing Agent	55:79	an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature	55:153	Design of a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature.					
35316068	0	32	theme	Antiplasticizing	58:73	arg1	Lactose					44:50	Lactose	44:50	Lactose	44:50	Design of a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature.					
35316068	15	33	theme	LAC	2247:2249	arg1	LAC					2247:2249	LAC	2247:2249	LAC	2247:2249	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC might have caused crystallization during storage.					
35316068	15	33	theme	LAC	2247:2249	arg1	%					2242:2242	10-50%	2237:2242	10-50% of LAC	2237:2249	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC might have caused crystallization during storage.					
35316068	2	34	theme	higher	404:409	arg1	Tgs					411:413	higher Tgs	404:413	higher Tgs	404:413	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	5	35	theme	neat	711:714	arg1	points					724:729	the neat melting points	707:729	the neat melting points depending on the composition of the physical mixtures	707:783	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	2	36	theme	carriers	390:397	arg1	lack					372:375	the lack	368:375	the lack of polymeric carriers with higher Tgs	368:413	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	5	37	theme	mixtures	776:783	arg1	composition					748:758	the composition	744:758	the composition of the physical mixtures	744:783	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	12	38	theme	Principal	1701:1709	arg1	analysis					1721:1728	Principal component analysis	1701:1728	Principal component analysis of the infrared spectra	1701:1752	Principal component analysis of the infrared spectra revealed a significant change at the 70:30 molar ratio of DPH to LAC, which was in agreement with the results of the thermal analysis.					
35316068	15	39	theme	coamorphous	2207:2217	arg1	system					2219:2224	the coamorphous system	2203:2224	the coamorphous system containing 10-50% of LAC	2203:2249	The relatively lower Tg (less than 40 °C) of the coamorphous system containing 10-50% of LAC might have caused crystallization during storage.					
35316068	16	40	theme	coamorphous	2411:2421	arg1	systems					2423:2429	coamorphous systems	2411:2429	coamorphous systems as an antiplasticizing coformer	2411:2461	These findings indicate that LAC, which is a safe and widely used pharmaceutical excipient, can be applied to coamorphous systems as an antiplasticizing coformer.					
35316068	8	41	dep	melting	1142:1148	arg1	the					1138:1140	the	1138:1140	the	1138:1140	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	8	42	theme	coamorphous	1275:1285	arg1	system					1287:1292	a coamorphous system	1273:1292	a coamorphous system	1273:1292	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	10	43	theme	dispersion	1496:1505	arg1	formation					1469:1477	the formation	1465:1477	the formation of a homogeneous dispersion of DPH and LAC in the coamorphous system	1465:1546	The Raman image indicated the formation of a homogeneous dispersion of DPH and LAC in the coamorphous system.					
35316068	7	44	theme	pure	1076:1079	arg1	components					1081:1090	the pure components	1072:1090	the pure components	1072:1090	The heat of fusion of the eutectic mixture was maximized at a 70:30 molar ratio of DPH to LAC; at this point, the melting peaks of the pure components disappeared.					
35316068	9	45	theme	molar	1412:1416	arg1	ratio					1418:1422	a 10:90 molar ratio	1404:1422	a 10:90 molar ratio of DPH to LAC	1404:1436	Lactose showed a Tg of over 100 °C, and the Tg of DPH increased with the molar ratio of LAC; it was 84 °C at a 10:90 molar ratio of DPH to LAC.					
35316068	9	46	from	ratio	1418:1422	arg1	°C					1398:1399	84 °C	1395:1399	84 °C	1395:1399	Lactose showed a Tg of over 100 °C, and the Tg of DPH increased with the molar ratio of LAC; it was 84 °C at a 10:90 molar ratio of DPH to LAC.					
35316068	14	47	contain	containing	2001:2010	arg2	%					2017:2017	10-50%	2012:2017	10-50% of LAC	2012:2024	The coamorphous samples containing 10-50% of LAC remained in an amorphous state for 21 days, and no crystallization was observed for the samples containing >60% of LAC for 28 days.					
35316068	14	47	contain	containing	2001:2010	arg2	LAC					2022:2024	LAC	2022:2024	LAC	2022:2024	The coamorphous samples containing 10-50% of LAC remained in an amorphous state for 21 days, and no crystallization was observed for the samples containing >60% of LAC for 28 days.					
35316068	14	47	contain	containing	2001:2010	arg1	samples					1993:1999	The coamorphous samples	1977:1999	The coamorphous samples containing 10-50% of LAC	1977:2024	The coamorphous samples containing 10-50% of LAC remained in an amorphous state for 21 days, and no crystallization was observed for the samples containing >60% of LAC for 28 days.					
35316068	13	48	from	°C	1912:1913	arg1	test					1901:1904	A stability test	1889:1904	A stability test at 40 °C	1889:1913	A stability test at 40 °C revealed rapid crystallization of the supercooled liquid DPH.					
35316068	0	49	theme	Coamorphous	19:29	arg1	System					31:36	a Stable Coamorphous System	10:36	a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature	10:153	Design of a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature.					
35316068	13	50	theme	DPH	1972:1974	arg1	crystallization					1930:1944	rapid crystallization	1924:1944	rapid crystallization of the supercooled liquid DPH	1924:1974	A stability test at 40 °C revealed rapid crystallization of the supercooled liquid DPH.					
35316068	16	51	theme	antiplasticizing	2437:2452	arg1	coformer					2454:2461	an antiplasticizing coformer	2434:2461	an antiplasticizing coformer	2434:2461	These findings indicate that LAC, which is a safe and widely used pharmaceutical excipient, can be applied to coamorphous systems as an antiplasticizing coformer.					
35316068	12	52	from	ratio	1803:1807	arg1	change					1777:1782	a significant change	1763:1782	a significant change	1763:1782	Principal component analysis of the infrared spectra revealed a significant change at the 70:30 molar ratio of DPH to LAC, which was in agreement with the results of the thermal analysis.					
35316068	10	53	theme	LAC	1518:1520	arg1	dispersion					1496:1505	a homogeneous dispersion	1482:1505	a homogeneous dispersion of DPH and LAC	1482:1520	The Raman image indicated the formation of a homogeneous dispersion of DPH and LAC in the coamorphous system.					
35316068	7	54	theme	melting	1055:1061	arg1	peaks					1063:1067	the melting peaks	1051:1067	the melting peaks of the pure components	1051:1090	The heat of fusion of the eutectic mixture was maximized at a 70:30 molar ratio of DPH to LAC; at this point, the melting peaks of the pure components disappeared.					
35316068	8	55	dep	90:10	1219:1223	arg1	to					1216:1217	to	1216:1217	to	1216:1217	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	10	56	from	formation	1469:1477	arg1	system					1541:1546	the coamorphous system	1525:1546	the coamorphous system	1525:1546	The Raman image indicated the formation of a homogeneous dispersion of DPH and LAC in the coamorphous system.					
35316068	10	57	theme	DPH	1510:1512	arg1	dispersion					1496:1505	a homogeneous dispersion	1482:1505	a homogeneous dispersion of DPH and LAC	1482:1520	The Raman image indicated the formation of a homogeneous dispersion of DPH and LAC in the coamorphous system.					
35316068	1	58	theme	polymeric	235:243	arg1	dispersions					251:261	polymeric solid dispersions	235:261	polymeric solid dispersions	235:261	Coamorphous systems comprising small molecules are emerging as counterparts to polymeric solid dispersions.					
35316068	16	59	theme	used	2362:2365	arg1	LAC					2330:2332	LAC	2330:2332	LAC	2330:2332	These findings indicate that LAC, which is a safe and widely used pharmaceutical excipient, can be applied to coamorphous systems as an antiplasticizing coformer.					
35316068	16	59	theme	used	2362:2365	arg1	excipient					2382:2390	a safe and widely used pharmaceutical excipient	2344:2390	a safe and widely used pharmaceutical excipient	2344:2390	These findings indicate that LAC, which is a safe and widely used pharmaceutical excipient, can be applied to coamorphous systems as an antiplasticizing coformer.					
35316068	4	60	theme	model	594:598	arg1	°C					614:615	Tg = 16 °C	606:615	Tg = 16 °C	606:615	Diphenhydramine hydrochloride (DPH) was selected as a model drug (Tg = 16 °C).					
35316068	4	60	theme	model	594:598	arg1	drug					600:603	a model drug	592:603	a model drug (Tg = 16 °C)	592:616	Diphenhydramine hydrochloride (DPH) was selected as a model drug (Tg = 16 °C).					
35316068	4	60	theme	model	594:598	arg1	hydrochloride					556:568	Diphenhydramine hydrochloride	540:568	Diphenhydramine hydrochloride (DPH)	540:574	Diphenhydramine hydrochloride (DPH) was selected as a model drug (Tg = 16 °C).					
35316068	12	61	theme	thermal	1871:1877	arg1	analysis					1879:1886	the thermal analysis	1867:1886	the thermal analysis	1867:1886	Principal component analysis of the infrared spectra revealed a significant change at the 70:30 molar ratio of DPH to LAC, which was in agreement with the results of the thermal analysis.					
35316068	9	62	theme	°C	1327:1328	arg1	Tg					1312:1313	a Tg	1310:1313	a Tg of over 100 °C	1310:1328	Lactose showed a Tg of over 100 °C, and the Tg of DPH increased with the molar ratio of LAC; it was 84 °C at a 10:90 molar ratio of DPH to LAC.					
35316068	13	63	theme	supercooled	1953:1963	arg1	DPH					1972:1974	the supercooled liquid DPH	1949:1974	the supercooled liquid DPH	1949:1974	A stability test at 40 °C revealed rapid crystallization of the supercooled liquid DPH.					
35316068	2	64	with	carriers	390:397	arg1	Tgs					411:413	higher Tgs	404:413	higher Tgs	404:413	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	3	65	theme	antiplasticizing	489:504	arg1	coformer					506:513	an antiplasticizing coformer	486:513	an antiplasticizing coformer to a coamorphous system	486:537	This study aimed to investigate the applicability of lactose (LAC) as an antiplasticizing coformer to a coamorphous system.					
35316068	6	66	theme	eutectic	864:871	arg1	mixture					873:879	the eutectic mixture	860:879	the eutectic mixture	860:879	The melting point of the eutectic mixture was calculated according to the Schröder-van Laar equation.					
35316068	14	67	theme	coamorphous	1981:1991	arg1	samples					1993:1999	The coamorphous samples	1977:1999	The coamorphous samples containing 10-50% of LAC	1977:2024	The coamorphous samples containing 10-50% of LAC remained in an amorphous state for 21 days, and no crystallization was observed for the samples containing >60% of LAC for 28 days.					
35316068	12	68	with	agreement	1837:1845	arg1	results					1856:1862	the results	1852:1862	the results of the thermal analysis	1852:1886	Principal component analysis of the infrared spectra revealed a significant change at the 70:30 molar ratio of DPH to LAC, which was in agreement with the results of the thermal analysis.					
35316068	5	69	theme	scanning	632:639	arg1	calorimetry					641:651	Differential scanning calorimetry	619:651	Differential scanning calorimetry	619:651	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	13	70	theme	rapid	1924:1928	arg1	crystallization					1930:1944	rapid crystallization	1924:1944	rapid crystallization of the supercooled liquid DPH	1924:1974	A stability test at 40 °C revealed rapid crystallization of the supercooled liquid DPH.					
35316068	12	71	theme	molar	1797:1801	arg1	ratio					1803:1807	the 70:30 molar ratio	1787:1807	the 70:30 molar ratio of DPH to LAC	1787:1821	Principal component analysis of the infrared spectra revealed a significant change at the 70:30 molar ratio of DPH to LAC, which was in agreement with the results of the thermal analysis.					
35316068	8	72	from	melting	1142:1148	arg1	ratios					1198:1203	the ratios	1194:1203	the ratios from 10:90 to 90:10	1194:1223	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	9	73	theme	LAC	1383:1385	arg1	ratio					1374:1378	the molar ratio	1364:1378	the molar ratio of LAC	1364:1385	Lactose showed a Tg of over 100 °C, and the Tg of DPH increased with the molar ratio of LAC; it was 84 °C at a 10:90 molar ratio of DPH to LAC.					
35316068	11	74	theme	DPH	1666:1668	arg1	DPH					1666:1668	DPH	1666:1668	DPH	1666:1668	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	11	74	theme	DPH	1666:1668	arg1	group					1657:1661	the amino group	1647:1661	the amino group of DPH	1647:1668	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	11	74	theme	DPH	1666:1668	arg1	group					1687:1691	the hydroxyl group	1674:1691	the hydroxyl group of LAC	1674:1698	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	11	74	theme	DPH	1666:1668	arg1	LAC					1696:1698	LAC	1696:1698	LAC	1696:1698	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	3	75	theme	coamorphous	520:530	arg1	system					532:537	a coamorphous system	518:537	a coamorphous system	518:537	This study aimed to investigate the applicability of lactose (LAC) as an antiplasticizing coformer to a coamorphous system.					
35316068	5	76	theme	DPH-LAC	817:823	arg1	mixture					806:812	the mixture	802:812	the mixture of DPH-LAC	802:823	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	5	76	theme	DPH-LAC	817:823	arg1	eutectic					829:836	eutectic	829:836	eutectic	829:836	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	6	77	theme	melting	843:849	arg1	point					851:855	The melting point	839:855	The melting point of the eutectic mixture	839:879	The melting point of the eutectic mixture was calculated according to the Schröder-van Laar equation.					
35316068	7	78	theme	DPH	1024:1026	arg1	ratio					1015:1019	a 70:30 molar ratio	1001:1019	a 70:30 molar ratio of DPH to LAC	1001:1033	The heat of fusion of the eutectic mixture was maximized at a 70:30 molar ratio of DPH to LAC; at this point, the melting peaks of the pure components disappeared.					
35316068	0	79	with	Hydrochloride	101:113	arg1	Temperature					143:153	a Low Glass Transition Temperature	120:153	a Low Glass Transition Temperature	120:153	Design of a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature.					
35316068	2	80	theme	materials	328:336	arg1	low					353:355	low	353:355	low	353:355	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	2	80	theme	materials	328:336	arg1	Tgs					308:310	Tgs	308:310	Tgs	308:310	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	2	80	theme	materials	328:336	arg1	temperatures					294:305	the glass transition temperatures	273:305	the glass transition temperatures (Tgs) of coamorphous materials	273:336	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	14	81	contain	containing	2122:2131	arg1	samples					2114:2120	the samples	2110:2120	the samples containing >60% of LAC for 28 days	2110:2155	The coamorphous samples containing 10-50% of LAC remained in an amorphous state for 21 days, and no crystallization was observed for the samples containing >60% of LAC for 28 days.					
35316068	14	81	contain	containing	2122:2131	arg2	LAC					2141:2143	LAC	2141:2143	LAC	2141:2143	The coamorphous samples containing 10-50% of LAC remained in an amorphous state for 21 days, and no crystallization was observed for the samples containing >60% of LAC for 28 days.					
35316068	14	81	contain	containing	2122:2131	arg2	%					2136:2136	>60%	2133:2136	>60% of LAC for 28 days	2133:2155	The coamorphous samples containing 10-50% of LAC remained in an amorphous state for 21 days, and no crystallization was observed for the samples containing >60% of LAC for 28 days.					
35316068	11	82	theme	intermolecular	1611:1624	arg1	interactions					1626:1637	intermolecular interactions	1611:1637	intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC	1611:1698	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	0	83	theme	Low	122:124	arg1	Temperature					143:153	a Low Glass Transition Temperature	120:153	a Low Glass Transition Temperature	120:153	Design of a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature.					
35316068	8	84	theme	heat	1109:1112	arg1	profiles					1119:1126	The heat flow profiles	1105:1126	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10	1105:1223	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	6	85	theme	Laar	926:929	arg1	equation					931:938	the Schröder-van Laar equation	909:938	the Schröder-van Laar equation	909:938	The melting point of the eutectic mixture was calculated according to the Schröder-van Laar equation.					
35316068	8	86	theme	physical	1173:1180	arg1	mixtures					1182:1189	DPH-LAC physical mixtures	1165:1189	DPH-LAC physical mixtures at the ratios from 10:90 to 90:10	1165:1223	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	10	87	theme	Raman	1443:1447	arg1	image					1449:1453	The Raman image	1439:1453	The Raman image	1439:1453	The Raman image indicated the formation of a homogeneous dispersion of DPH and LAC in the coamorphous system.					
35316068	0	88	theme	Transition	132:141	arg1	Temperature					143:153	a Low Glass Transition Temperature	120:153	a Low Glass Transition Temperature	120:153	Design of a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature.					
35316068	12	89	theme	spectra	1746:1752	arg1	analysis					1721:1728	Principal component analysis	1701:1728	Principal component analysis of the infrared spectra	1701:1752	Principal component analysis of the infrared spectra revealed a significant change at the 70:30 molar ratio of DPH to LAC, which was in agreement with the results of the thermal analysis.					
35316068	12	90	theme	70:30	1791:1795	arg1	ratio					1803:1807	the 70:30 molar ratio	1787:1807	the 70:30 molar ratio of DPH to LAC	1787:1821	Principal component analysis of the infrared spectra revealed a significant change at the 70:30 molar ratio of DPH to LAC, which was in agreement with the results of the thermal analysis.					
35316068	11	91	theme	amino	1651:1655	arg1	DPH					1666:1668	DPH	1666:1668	DPH	1666:1668	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	11	91	theme	amino	1651:1655	arg1	group					1657:1661	the amino group	1647:1661	the amino group of DPH	1647:1668	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	11	91	theme	amino	1651:1655	arg1	LAC					1696:1698	LAC	1696:1698	LAC	1696:1698	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	7	92	theme	mixture	976:982	arg1	fusion					953:958	fusion	953:958	fusion of the eutectic mixture	953:982	The heat of fusion of the eutectic mixture was maximized at a 70:30 molar ratio of DPH to LAC; at this point, the melting peaks of the pure components disappeared.					
35316068	16	93	theme	safe	2346:2349	arg1	LAC					2330:2332	LAC	2330:2332	LAC	2330:2332	These findings indicate that LAC, which is a safe and widely used pharmaceutical excipient, can be applied to coamorphous systems as an antiplasticizing coformer.					
35316068	16	93	theme	safe	2346:2349	arg1	excipient					2382:2390	a safe and widely used pharmaceutical excipient	2344:2390	a safe and widely used pharmaceutical excipient	2344:2390	These findings indicate that LAC, which is a safe and widely used pharmaceutical excipient, can be applied to coamorphous systems as an antiplasticizing coformer.					
35316068	5	94	theme	comelting	662:670	arg1	point					672:676	a comelting point	660:676	a comelting point	660:676	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	2	95	theme	glass	277:281	arg1	low					353:355	low	353:355	low	353:355	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	2	95	theme	glass	277:281	arg1	Tgs					308:310	Tgs	308:310	Tgs	308:310	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	2	95	theme	glass	277:281	arg1	temperatures					294:305	the glass transition temperatures	273:305	the glass transition temperatures (Tgs) of coamorphous materials	273:336	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	5	96	theme	melting	716:722	arg1	points					724:729	the neat melting points	707:729	the neat melting points depending on the composition of the physical mixtures	707:783	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	5	97	from	points	724:729	arg1	composition					748:758	the composition	744:758	the composition of the physical mixtures	744:783	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	7	98	theme	fusion	953:958	arg1	heat					945:948	The heat	941:948	The heat of fusion of the eutectic mixture	941:982	The heat of fusion of the eutectic mixture was maximized at a 70:30 molar ratio of DPH to LAC; at this point, the melting peaks of the pure components disappeared.					
35316068	11	99	theme	Peak	1549:1552	arg1	shifts					1554:1559	Peak shifts	1549:1559	Peak shifts in the infrared spectra	1549:1583	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	1	100	theme	Coamorphous	156:166	arg1	systems					168:174	Coamorphous systems	156:174	Coamorphous systems comprising small molecules	156:201	Coamorphous systems comprising small molecules are emerging as counterparts to polymeric solid dispersions.					
35316068	0	101	theme	Diphenhydramine	85:99	arg1	Hydrochloride					101:113	Diphenhydramine Hydrochloride	85:113	Diphenhydramine Hydrochloride with a Low Glass Transition Temperature	85:153	Design of a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature.					
35316068	5	102	from	composition	748:758	arg1	decrease					695:702	a decrease	693:702	a decrease in the neat melting points depending on the composition of the physical mixtures	693:783	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	12	103	theme	infrared	1737:1744	arg1	spectra					1746:1752	the infrared spectra	1733:1752	the infrared spectra	1733:1752	Principal component analysis of the infrared spectra revealed a significant change at the 70:30 molar ratio of DPH to LAC, which was in agreement with the results of the thermal analysis.					
35316068	4	104	theme	=	609:609	arg1	°C					614:615	Tg = 16 °C	606:615	Tg = 16 °C	606:615	Diphenhydramine hydrochloride (DPH) was selected as a model drug (Tg = 16 °C).					
35316068	4	104	theme	=	609:609	arg1	drug					600:603	a model drug	592:603	a model drug (Tg = 16 °C)	592:616	Diphenhydramine hydrochloride (DPH) was selected as a model drug (Tg = 16 °C).					
35316068	11	105	theme	infrared	1568:1575	arg1	spectra					1577:1583	the infrared spectra	1564:1583	the infrared spectra	1564:1583	Peak shifts in the infrared spectra indicated the presence of intermolecular interactions between the amino group of DPH and the hydroxyl group of LAC.					
35316068	8	106	from	ratios	1198:1203	arg1	mixtures					1182:1189	DPH-LAC physical mixtures	1165:1189	DPH-LAC physical mixtures at the ratios from 10:90 to 90:10	1165:1223	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	8	106	from	ratios	1198:1203	arg1	cooling					1154:1160	cooling	1154:1160	cooling	1154:1160	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	8	106	from	ratios	1198:1203	arg1	melting					1142:1148	melting	1142:1148	melting	1142:1148	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	14	107	theme	amorphous	2041:2049	arg1	state					2051:2055	an amorphous state	2038:2055	an amorphous state for 21 days	2038:2067	The coamorphous samples containing 10-50% of LAC remained in an amorphous state for 21 days, and no crystallization was observed for the samples containing >60% of LAC for 28 days.					
35316068	5	108	theme	physical	767:774	arg1	mixtures					776:783	the physical mixtures	763:783	the physical mixtures	763:783	Differential scanning calorimetry showed a comelting point in addition to a decrease in the neat melting points depending on the composition of the physical mixtures, suggesting that the mixture of DPH-LAC was eutectic.					
35316068	12	109	theme	component	1711:1719	arg1	analysis					1721:1728	Principal component analysis	1701:1728	Principal component analysis of the infrared spectra	1701:1752	Principal component analysis of the infrared spectra revealed a significant change at the 70:30 molar ratio of DPH to LAC, which was in agreement with the results of the thermal analysis.					
35316068	7	110	theme	70:30	1003:1007	arg1	ratio					1015:1019	a 70:30 molar ratio	1001:1019	a 70:30 molar ratio of DPH to LAC	1001:1033	The heat of fusion of the eutectic mixture was maximized at a 70:30 molar ratio of DPH to LAC; at this point, the melting peaks of the pure components disappeared.					
35316068	2	111	theme	polymeric	380:388	arg1	carriers					390:397	polymeric carriers	380:397	polymeric carriers with higher Tgs	380:413	However, the glass transition temperatures (Tgs) of coamorphous materials are relatively low because of the lack of polymeric carriers with higher Tgs.					
35316068	0	112	theme	Stable	12:17	arg1	System					31:36	a Stable Coamorphous System	10:36	a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature	10:153	Design of a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature.					
35316068	8	113	from	90:10	1219:1223	arg1	ratios					1198:1203	the ratios	1194:1203	the ratios from 10:90 to 90:10	1194:1223	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	14	114	theme	LAC	2022:2024	arg1	LAC					2022:2024	LAC	2022:2024	LAC	2022:2024	The coamorphous samples containing 10-50% of LAC remained in an amorphous state for 21 days, and no crystallization was observed for the samples containing >60% of LAC for 28 days.					
35316068	14	114	theme	LAC	2022:2024	arg1	%					2017:2017	10-50%	2012:2017	10-50% of LAC	2012:2024	The coamorphous samples containing 10-50% of LAC remained in an amorphous state for 21 days, and no crystallization was observed for the samples containing >60% of LAC for 28 days.					
35316068	10	115	theme	homogeneous	1484:1494	arg1	dispersion					1496:1505	a homogeneous dispersion	1482:1505	a homogeneous dispersion of DPH and LAC	1482:1520	The Raman image indicated the formation of a homogeneous dispersion of DPH and LAC in the coamorphous system.					
35316068	0	116	theme	System	31:36	arg1	Design					0:5	Design	0:5	Design of a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature.	0:154	Design of a Stable Coamorphous System Using Lactose as an Antiplasticizing Agent for Diphenhydramine Hydrochloride with a Low Glass Transition Temperature.					
35316068	8	117	theme	system	1287:1292	arg1	formation					1260:1268	the formation	1256:1268	the formation of a coamorphous system	1256:1292	The heat flow profiles following the melting and cooling of DPH-LAC physical mixtures at the ratios from 10:90 to 90:10 showed a single Tg, suggesting the formation of a coamorphous system.					
35316068	9	118	theme	DPH	1427:1429	arg1	ratio					1418:1422	a 10:90 molar ratio	1404:1422	a 10:90 molar ratio of DPH to LAC	1404:1436	Lactose showed a Tg of over 100 °C, and the Tg of DPH increased with the molar ratio of LAC; it was 84 °C at a 10:90 molar ratio of DPH to LAC.					
35316068	7	119	theme	molar	1009:1013	arg1	ratio					1015:1019	a 70:30 molar ratio	1001:1019	a 70:30 molar ratio of DPH to LAC	1001:1033	The heat of fusion of the eutectic mixture was maximized at a 70:30 molar ratio of DPH to LAC; at this point, the melting peaks of the pure components disappeared.					
35316068	4	120	theme	Diphenhydramine	540:554	arg1	drug					600:603	a model drug	592:603	a model drug (Tg = 16 °C)	592:616	Diphenhydramine hydrochloride (DPH) was selected as a model drug (Tg = 16 °C).					
35316068	4	120	theme	Diphenhydramine	540:554	arg1	DPH					571:573	DPH	571:573	DPH	571:573	Diphenhydramine hydrochloride (DPH) was selected as a model drug (Tg = 16 °C).					
35316068	4	120	theme	Diphenhydramine	540:554	arg1	hydrochloride					556:568	Diphenhydramine hydrochloride	540:568	Diphenhydramine hydrochloride (DPH)	540:574	Diphenhydramine hydrochloride (DPH) was selected as a model drug (Tg = 16 °C).					
35316068	16	121	theme	pharmaceutical	2367:2380	arg1	LAC					2330:2332	LAC	2330:2332	LAC	2330:2332	These findings indicate that LAC, which is a safe and widely used pharmaceutical excipient, can be applied to coamorphous systems as an antiplasticizing coformer.					
35316068	16	121	theme	pharmaceutical	2367:2380	arg1	excipient					2382:2390	a safe and widely used pharmaceutical excipient	2344:2390	a safe and widely used pharmaceutical excipient	2344:2390	These findings indicate that LAC, which is a safe and widely used pharmaceutical excipient, can be applied to coamorphous systems as an antiplasticizing coformer.					
35316068	1	122	theme	solid	245:249	arg1	dispersions					251:261	polymeric solid dispersions	235:261	polymeric solid dispersions	235:261	Coamorphous systems comprising small molecules are emerging as counterparts to polymeric solid dispersions.					
35316068	4	123	theme	Tg	606:607	arg1	°C					614:615	Tg = 16 °C	606:615	Tg = 16 °C	606:615	Diphenhydramine hydrochloride (DPH) was selected as a model drug (Tg = 16 °C).					
35316068	4	123	theme	Tg	606:607	arg1	drug					600:603	a model drug	592:603	a model drug (Tg = 16 °C)	592:616	Diphenhydramine hydrochloride (DPH) was selected as a model drug (Tg = 16 °C).					
35316068	10	124	theme	coamorphous	1529:1539	arg1	system					1541:1546	the coamorphous system	1525:1546	the coamorphous system	1525:1546	The Raman image indicated the formation of a homogeneous dispersion of DPH and LAC in the coamorphous system.					
35316068	13	125	theme	liquid	1965:1970	arg1	DPH					1972:1974	the supercooled liquid DPH	1949:1974	the supercooled liquid DPH	1949:1974	A stability test at 40 °C revealed rapid crystallization of the supercooled liquid DPH.					
36209877	0	0	theme	oil	99:101	arg1	wastewater					103:112	emulsified oil wastewater	88:112	emulsified oil wastewater	88:112	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater containing Pb2+ ions.					
36209877	4	1	theme	metal	869:873	arg1	ions					875:878	metal ions	869:878	metal ions	869:878	The PVA-CS-LDH membrane can purify emulsified oil and metal ions simultaneously with a separation efficiency of 99.89 % for emulsified oil and a removal efficiency of 97.44 % for Pb2+ ions.					
36209877	5	2	theme	membrane	1120:1127	arg1	properties					1091:1100	the high-efficiency, multifunctional, high-antifouling and eco-friendly properties	1019:1100	the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane	1019:1127	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	1	3	theme	engineering	159:169	arg1	materials					171:179	most existing engineering materials	145:179	most existing engineering materials	145:179	To date, most existing engineering materials have difficulty simultaneously separating oil/water and removing heavy metals from complex oily wastewater.					
36209877	5	4	theme	heavy	1206:1210	arg1	removal					1222:1228	heavy metal ion removal	1206:1228	heavy metal ion removal	1206:1228	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	0	5	theme	emulsified	88:97	arg1	wastewater					103:112	emulsified oil wastewater	88:112	emulsified oil wastewater	88:112	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater containing Pb2+ ions.					
36209877	5	6	theme	multifunctional	1040:1054	arg1	properties					1091:1100	the high-efficiency, multifunctional, high-antifouling and eco-friendly properties	1019:1100	the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane	1019:1127	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	2	7	dep	challenge	309:317	arg1	response					292:299	response	292:299	response	292:299	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	5	8	theme	metal	1212:1216	arg1	removal					1222:1228	heavy metal ion removal	1206:1228	heavy metal ion removal	1206:1228	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	4	9	theme	emulsified	939:948	arg1	oil					950:952	emulsified oil	939:952	emulsified oil	939:952	The PVA-CS-LDH membrane can purify emulsified oil and metal ions simultaneously with a separation efficiency of 99.89 % for emulsified oil and a removal efficiency of 97.44 % for Pb2+ ions.					
36209877	5	10	theme	oil	1187:1189	arg1	separation					1191:1200	emulsified oil separation	1176:1200	emulsified oil separation	1176:1200	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	3	11	theme	easy	544:547	arg1	strategy					563:570	an easy yet versatile strategy	541:570	an easy yet versatile strategy	541:570	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	3	12	with	PVA-CS-LDHs	692:702	arg1	recoverability					767:780	favourable shape recoverability	750:780	favourable shape recoverability	750:780	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	3	12	with	PVA-CS-LDHs	692:702	arg1	microstructure					799:812	an ideal 3D microstructure	787:812	an ideal 3D microstructure	787:812	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	3	12	with	PVA-CS-LDHs	692:702	arg1	strength					714:721	high strength	709:721	high strength	709:721	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	3	12	with	PVA-CS-LDHs	692:702	arg1	stretchability					734:747	excellent stretchability	724:747	excellent stretchability	724:747	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	5	13	theme	ion	1218:1220	arg1	removal					1222:1228	heavy metal ion removal	1206:1228	heavy metal ion removal	1206:1228	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	0	14	theme	wastewater	103:112	arg1	purification					72:83	the efficient purification	58:83	the efficient purification of emulsified oil wastewater	58:112	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater containing Pb2+ ions.					
36209877	4	15	theme	%	988:988	arg1	efficiency					968:977	a removal efficiency	958:977	a removal efficiency of 97.44 % for Pb2+ ions	958:1002	The PVA-CS-LDH membrane can purify emulsified oil and metal ions simultaneously with a separation efficiency of 99.89 % for emulsified oil and a removal efficiency of 97.44 % for Pb2+ ions.					
36209877	4	15	theme	%	988:988	arg1	oil					950:952	emulsified oil	939:952	emulsified oil	939:952	The PVA-CS-LDH membrane can purify emulsified oil and metal ions simultaneously with a separation efficiency of 99.89 % for emulsified oil and a removal efficiency of 97.44 % for Pb2+ ions.					
36209877	6	16	theme	scientific	1308:1317	arg1	challenges					1337:1346	scientific and technological challenges	1308:1346	scientific and technological challenges	1308:1346	Thus, this material provides critical application potential that can address scientific and technological challenges in complex oily wastewater purification.					
36209877	6	17	theme	application	1269:1279	arg1	potential					1281:1289	critical application potential	1260:1289	critical application potential that can address scientific and technological challenges in complex oily wastewater purification	1260:1386	Thus, this material provides critical application potential that can address scientific and technological challenges in complex oily wastewater purification.					
36209877	0	18	theme	Pb2+	125:128	arg1	ions					130:133	Pb2+ ions	125:133	Pb2+ ions	125:133	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater containing Pb2+ ions.					
36209877	5	19	theme	PVA-CS-LDH	1109:1118	arg1	membrane					1120:1127	the PVA-CS-LDH membrane	1105:1127	the PVA-CS-LDH membrane	1105:1127	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	6	20	theme	critical	1260:1267	arg1	potential					1281:1289	critical application potential	1260:1289	critical application potential that can address scientific and technological challenges in complex oily wastewater purification	1260:1386	Thus, this material provides critical application potential that can address scientific and technological challenges in complex oily wastewater purification.					
36209877	6	21	theme	complex	1351:1357	arg1	purification					1375:1386	complex oily wastewater purification	1351:1386	complex oily wastewater purification	1351:1386	Thus, this material provides critical application potential that can address scientific and technological challenges in complex oily wastewater purification.					
36209877	4	22	theme	removal	960:966	arg1	efficiency					968:977	a removal efficiency	958:977	a removal efficiency of 97.44 % for Pb2+ ions	958:1002	The PVA-CS-LDH membrane can purify emulsified oil and metal ions simultaneously with a separation efficiency of 99.89 % for emulsified oil and a removal efficiency of 97.44 % for Pb2+ ions.					
36209877	0	23	theme	multifunctional	6:20	arg1	hydrogel-membrane					36:52	Green multifunctional PVA composite hydrogel-membrane	0:52	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater	0:112	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater containing Pb2+ ions.					
36209877	3	24	theme	high	709:712	arg1	strength					714:721	high strength	709:721	high strength	709:721	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	5	25	theme	high-antifouling	1057:1072	arg1	properties					1091:1100	the high-efficiency, multifunctional, high-antifouling and eco-friendly properties	1019:1100	the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane	1019:1127	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	2	26	theme	composite	344:352	arg1	membrane					363:370	a novel multifunctional composite hydrogel membrane	320:370	a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs)	320:390	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	2	26	theme	composite	344:352	arg1	PVA-CS-LDHs					379:389	named PVA-CS-LDHs	373:389	named PVA-CS-LDHs	373:389	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	3	27	from	formation	622:630	arg1	step					673:676	one step	669:676	one step	669:676	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	0	28	theme	Green	0:4	arg1	hydrogel-membrane					36:52	Green multifunctional PVA composite hydrogel-membrane	0:52	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater	0:112	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater containing Pb2+ ions.					
36209877	5	29	theme	emulsified	1176:1185	arg1	separation					1191:1200	emulsified oil separation	1176:1200	emulsified oil separation	1176:1200	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	4	30	theme	emulsified	850:859	arg1	oil					861:863	emulsified oil	850:863	emulsified oil	850:863	The PVA-CS-LDH membrane can purify emulsified oil and metal ions simultaneously with a separation efficiency of 99.89 % for emulsified oil and a removal efficiency of 97.44 % for Pb2+ ions.					
36209877	2	31	theme	multifunctional	328:342	arg1	membrane					363:370	a novel multifunctional composite hydrogel membrane	320:370	a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs)	320:390	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	2	31	theme	multifunctional	328:342	arg1	PVA-CS-LDHs					379:389	named PVA-CS-LDHs	373:389	named PVA-CS-LDHs	373:389	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	0	32	theme	composite	26:34	arg1	hydrogel-membrane					36:52	Green multifunctional PVA composite hydrogel-membrane	0:52	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater	0:112	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater containing Pb2+ ions.					
36209877	3	33	theme	favourable	750:759	arg1	recoverability					767:780	favourable shape recoverability	750:780	favourable shape recoverability	750:780	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	6	34	theme	wastewater	1364:1373	arg1	purification					1375:1386	complex oily wastewater purification	1351:1386	complex oily wastewater purification	1351:1386	Thus, this material provides critical application potential that can address scientific and technological challenges in complex oily wastewater purification.					
36209877	3	35	theme	salting-out	588:598	arg1	strategy					563:570	an easy yet versatile strategy	541:570	an easy yet versatile strategy	541:570	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	2	36	theme	novel	322:326	arg1	membrane					363:370	a novel multifunctional composite hydrogel membrane	320:370	a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs)	320:390	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	2	36	theme	novel	322:326	arg1	PVA-CS-LDHs					379:389	named PVA-CS-LDHs	373:389	named PVA-CS-LDHs	373:389	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	0	37	theme	PVA	22:24	arg1	hydrogel-membrane					36:52	Green multifunctional PVA composite hydrogel-membrane	0:52	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater	0:112	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater containing Pb2+ ions.					
36209877	3	38	theme	excellent	724:732	arg1	stretchability					734:747	excellent stretchability	724:747	excellent stretchability	724:747	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	6	39	theme	oily	1359:1362	arg1	purification					1375:1386	complex oily wastewater purification	1351:1386	complex oily wastewater purification	1351:1386	Thus, this material provides critical application potential that can address scientific and technological challenges in complex oily wastewater purification.					
36209877	3	40	theme	PVA-CS-LDH	637:646	arg1	membrane					657:664	a PVA-CS-LDH hydrogel membrane	635:664	a PVA-CS-LDH hydrogel membrane in one step	635:676	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	3	41	from	step	673:676	arg1	formation					622:630	the formation	618:630	the formation of a PVA-CS-LDH hydrogel membrane in one step	618:676	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	3	42	theme	hydrogel	648:655	arg1	membrane					657:664	a PVA-CS-LDH hydrogel membrane	635:664	a PVA-CS-LDH hydrogel membrane in one step	635:676	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	3	43	theme	freezing	575:582	arg1	strategy					563:570	an easy yet versatile strategy	541:570	an easy yet versatile strategy	541:570	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	2	44	theme	alcohol	483:489	arg1	hydrogel					497:504	a polyvinyl alcohol (PVA) hydrogel	471:504	a polyvinyl alcohol (PVA) hydrogel	471:504	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	5	45	theme	hydrogel	1149:1156	arg1	material					1158:1165	a promising hydrogel material	1137:1165	a promising hydrogel material for both emulsified oil separation and heavy metal ion removal	1137:1228	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	4	46	theme	%	933:933	arg1	efficiency					913:922	a separation efficiency	900:922	a separation efficiency of 99.89 % for emulsified oil and a removal efficiency of 97.44 % for Pb2+ ions	900:1002	The PVA-CS-LDH membrane can purify emulsified oil and metal ions simultaneously with a separation efficiency of 99.89 % for emulsified oil and a removal efficiency of 97.44 % for Pb2+ ions.					
36209877	5	47	theme	eco-friendly	1078:1089	arg1	properties					1091:1100	the high-efficiency, multifunctional, high-antifouling and eco-friendly properties	1019:1100	the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane	1019:1127	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	1	48	theme	heavy	246:250	arg1	metals					252:257	heavy metals	246:257	heavy metals	246:257	To date, most existing engineering materials have difficulty simultaneously separating oil/water and removing heavy metals from complex oily wastewater.					
36209877	0	49	theme	efficient	62:70	arg1	purification					72:83	the efficient purification	58:83	the efficient purification of emulsified oil wastewater	58:112	Green multifunctional PVA composite hydrogel-membrane for the efficient purification of emulsified oil wastewater containing Pb2+ ions.					
36209877	4	50	theme	PVA-CS-LDH	819:828	arg1	membrane					830:837	The PVA-CS-LDH membrane	815:837	The PVA-CS-LDH membrane	815:837	The PVA-CS-LDH membrane can purify emulsified oil and metal ions simultaneously with a separation efficiency of 99.89 % for emulsified oil and a removal efficiency of 97.44 % for Pb2+ ions.					
36209877	1	51	contain	have	181:184	arg2	difficulty					186:195	difficulty	186:195	difficulty simultaneously separating oil/water and removing heavy metals from complex oily wastewater	186:286	To date, most existing engineering materials have difficulty simultaneously separating oil/water and removing heavy metals from complex oily wastewater.					
36209877	1	51	contain	have	181:184	arg1	materials					171:179	most existing engineering materials	145:179	most existing engineering materials	145:179	To date, most existing engineering materials have difficulty simultaneously separating oil/water and removing heavy metals from complex oily wastewater.					
36209877	2	52	theme	named	373:377	arg1	membrane					363:370	a novel multifunctional composite hydrogel membrane	320:370	a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs)	320:390	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	2	52	theme	named	373:377	arg1	PVA-CS-LDHs					379:389	named PVA-CS-LDHs	373:389	named PVA-CS-LDHs	373:389	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	4	53	theme	Pb2+	994:997	arg1	ions					999:1002	Pb2+ ions	994:1002	Pb2+ ions	994:1002	The PVA-CS-LDH membrane can purify emulsified oil and metal ions simultaneously with a separation efficiency of 99.89 % for emulsified oil and a removal efficiency of 97.44 % for Pb2+ ions.					
36209877	3	54	theme	shape	761:765	arg1	recoverability					767:780	favourable shape recoverability	750:780	favourable shape recoverability	750:780	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	6	55	theme	technological	1323:1335	arg1	challenges					1337:1346	scientific and technological challenges	1308:1346	scientific and technological challenges	1308:1346	Thus, this material provides critical application potential that can address scientific and technological challenges in complex oily wastewater purification.					
36209877	5	56	theme	high-efficiency	1023:1037	arg1	properties					1091:1100	the high-efficiency, multifunctional, high-antifouling and eco-friendly properties	1019:1100	the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane	1019:1127	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	1	57	dep	difficulty	186:195	arg1	separating					212:221	separating	212:221	simultaneously separating oil/water	197:231	To date, most existing engineering materials have difficulty simultaneously separating oil/water and removing heavy metals from complex oily wastewater.					
36209877	1	57	dep	difficulty	186:195	arg1	removing					237:244	removing	237:244	removing heavy metals from complex oily wastewater	237:286	To date, most existing engineering materials have difficulty simultaneously separating oil/water and removing heavy metals from complex oily wastewater.					
36209877	3	58	theme	versatile	553:561	arg1	strategy					563:570	an easy yet versatile strategy	541:570	an easy yet versatile strategy	541:570	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	3	59	theme	3D	796:797	arg1	microstructure					799:812	an ideal 3D microstructure	787:812	an ideal 3D microstructure	787:812	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	4	60	theme	separation	902:911	arg1	efficiency					913:922	a separation efficiency	900:922	a separation efficiency of 99.89 % for emulsified oil and a removal efficiency of 97.44 % for Pb2+ ions	900:1002	The PVA-CS-LDH membrane can purify emulsified oil and metal ions simultaneously with a separation efficiency of 99.89 % for emulsified oil and a removal efficiency of 97.44 % for Pb2+ ions.					
36209877	3	61	theme	membrane	657:664	arg1	formation					622:630	the formation	618:630	the formation of a PVA-CS-LDH hydrogel membrane in one step	618:676	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	2	62	theme	hydrogel	354:361	arg1	membrane					363:370	a novel multifunctional composite hydrogel membrane	320:370	a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs)	320:390	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	2	62	theme	hydrogel	354:361	arg1	PVA-CS-LDHs					379:389	named PVA-CS-LDHs	373:389	named PVA-CS-LDHs	373:389	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	3	63	from	membrane	657:664	arg1	step					673:676	one step	669:676	one step	669:676	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	5	64	theme	promising	1139:1147	arg1	material					1158:1165	a promising hydrogel material	1137:1165	a promising hydrogel material for both emulsified oil separation and heavy metal ion removal	1137:1228	Additionally, the high-efficiency, multifunctional, high-antifouling and eco-friendly properties of the PVA-CS-LDH membrane make it a promising hydrogel material for both emulsified oil separation and heavy metal ion removal.					
36209877	1	65	theme	most	145:148	arg1	materials					171:179	most existing engineering materials	145:179	most existing engineering materials	145:179	To date, most existing engineering materials have difficulty simultaneously separating oil/water and removing heavy metals from complex oily wastewater.					
36209877	1	66	theme	complex	264:270	arg1	wastewater					277:286	complex oily wastewater	264:286	complex oily wastewater	264:286	To date, most existing engineering materials have difficulty simultaneously separating oil/water and removing heavy metals from complex oily wastewater.					
36209877	2	67	theme	polyvinyl	473:481	arg1	PVA					492:494	PVA	492:494	PVA	492:494	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	2	67	theme	polyvinyl	473:481	arg1	alcohol					483:489	polyvinyl alcohol	473:489	a polyvinyl alcohol (PVA) hydrogel	471:504	In response to this challenge, a novel multifunctional composite hydrogel membrane (named PVA-CS-LDHs) was fabricated by incorporating chitosan (CS) and nanohydrotalcite (LDHs) into a polyvinyl alcohol (PVA) hydrogel.					
36209877	3	68	theme	ideal	790:794	arg1	microstructure					799:812	an ideal 3D microstructure	787:812	an ideal 3D microstructure	787:812	This material was developed using an easy yet versatile strategy of freezing and salting-out, which can enable the formation of a PVA-CS-LDH hydrogel membrane in one step and endow the PVA-CS-LDHs with high strength, excellent stretchability, favourable shape recoverability, and an ideal 3D microstructure.					
36209877	1	69	theme	existing	150:157	arg1	materials					171:179	most existing engineering materials	145:179	most existing engineering materials	145:179	To date, most existing engineering materials have difficulty simultaneously separating oil/water and removing heavy metals from complex oily wastewater.					
36209877	1	70	theme	oily	272:275	arg1	wastewater					277:286	complex oily wastewater	264:286	complex oily wastewater	264:286	To date, most existing engineering materials have difficulty simultaneously separating oil/water and removing heavy metals from complex oily wastewater.					
36604081	4	0	theme	bimodal	688:694	arg1	structure					701:709	a bimodal cell structure	686:709	a bimodal cell structure	686:709	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	4	1	with	foam	676:679	arg1	structure					701:709	a bimodal cell structure	686:709	a bimodal cell structure	686:709	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	1	2	theme	innovative	131:140	arg1	foam					164:167	an innovative PLA/CNF nanocomposite foam	128:167	an innovative PLA/CNF nanocomposite foam with a bimodal cell structure	128:197	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	1	3	theme	one-step	223:230	arg1	process					257:263	a simple one-step depressurization foaming process	214:263	a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent	214:332	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	0	4	theme	lactic	70:75	arg1	poly					64:67	bimodal poly	56:67	bimodal poly (lactic acid)	56:81	Improved thermal insulation and compressive property of bimodal poly (lactic acid)/cellulose nanocomposite foams.					
36604081	0	4	theme	lactic	70:75	arg1	acid					77:80	lactic acid	70:80	lactic acid	70:80	Improved thermal insulation and compressive property of bimodal poly (lactic acid)/cellulose nanocomposite foams.					
36604081	1	5	theme	foaming	320:326	arg1	agent					328:332	the foaming agent	316:332	the foaming agent	316:332	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	1	5	theme	foaming	320:326	arg1	dioxide					297:303	only supercritical carbon dioxide	271:303	only supercritical carbon dioxide (ScCO2)	271:311	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	5	6	theme	nanocomposite	893:905	arg1	foam					907:910	the PLA/CNF nanocomposite foam	881:910	the PLA/CNF nanocomposite foam	881:910	Meanwhile, the PLA/CNF nanocomposite foam exhibited excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials.					
36604081	5	6	theme	nanocomposite	893:905	arg1	Meanwhile					870:878	Meanwhile	870:878	Meanwhile	870:878	Meanwhile, the PLA/CNF nanocomposite foam exhibited excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials.					
36604081	1	7	theme	PLA/CNF	142:148	arg1	foam					164:167	an innovative PLA/CNF nanocomposite foam	128:167	an innovative PLA/CNF nanocomposite foam with a bimodal cell structure	128:197	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	1	8	theme	depressurization	232:247	arg1	process					257:263	a simple one-step depressurization foaming process	214:263	a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent	214:332	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	5	9	theme	promising	998:1006	arg1	application					1008:1018	promising application	998:1018	promising application in packaging and construction materials	998:1058	Meanwhile, the PLA/CNF nanocomposite foam exhibited excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials.					
36604081	3	10	theme	samples	533:539	arg1	kinetics					495:502	the different crystallization kinetics	465:502	the different crystallization kinetics	465:502	According to the different crystallization kinetics and nucleation efficiency of samples, it was inferred that the crystallization rate and phase interface would affect the cell structure.					
36604081	3	10	theme	samples	533:539	arg1	efficiency					519:528	nucleation efficiency	508:528	nucleation efficiency of samples	508:539	According to the different crystallization kinetics and nucleation efficiency of samples, it was inferred that the crystallization rate and phase interface would affect the cell structure.					
36604081	4	11	theme	prepared	645:652	arg1	foam					676:679	The prepared PLA/CNF nanocomposite foam	641:679	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure	641:709	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	1	12	theme	foaming	249:255	arg1	process					257:263	a simple one-step depressurization foaming process	214:263	a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent	214:332	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	4	13	contain	had	711:713	arg1	foam					676:679	The prepared PLA/CNF nanocomposite foam	641:679	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure	641:709	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	4	13	contain	had	711:713	arg2	ratio					728:732	an expansion ratio	715:732	an expansion ratio	715:732	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	5	14	theme	CNFs	979:982	arg1	presence					967:974	the presence	963:974	the presence of CNFs, which showed promising application in packaging and construction materials	963:1058	Meanwhile, the PLA/CNF nanocomposite foam exhibited excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials.					
36604081	3	15	theme	crystallization	479:493	arg1	kinetics					495:502	the different crystallization kinetics	465:502	the different crystallization kinetics	465:502	According to the different crystallization kinetics and nucleation efficiency of samples, it was inferred that the crystallization rate and phase interface would affect the cell structure.					
36604081	5	16	from	application	1008:1018	arg1	construction					1037:1048	construction	1037:1048	construction	1037:1048	Meanwhile, the PLA/CNF nanocomposite foam exhibited excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials.					
36604081	5	16	from	application	1008:1018	arg1	packaging					1023:1031	packaging	1023:1031	packaging	1023:1031	Meanwhile, the PLA/CNF nanocomposite foam exhibited excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials.					
36604081	4	17	theme	nanocomposite	662:674	arg1	foam					676:679	The prepared PLA/CNF nanocomposite foam	641:679	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure	641:709	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	1	18	theme	nanocomposite	150:162	arg1	foam					164:167	an innovative PLA/CNF nanocomposite foam	128:167	an innovative PLA/CNF nanocomposite foam with a bimodal cell structure	128:197	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	0	19	theme	nanocomposite	93:105	arg1	foams					107:111	nanocomposite foams	93:111	nanocomposite foams	93:111	Improved thermal insulation and compressive property of bimodal poly (lactic acid)/cellulose nanocomposite foams.					
36604081	2	20	theme	cell	418:421	arg1	structure					423:431	a bimodal cell structure	408:431	a bimodal cell structure	408:431	Only at a specific foaming temperature, PLA/CNF nanocomposites foam with a bimodal cell structure could be obtained.					
36604081	0	21	theme	thermal	9:15	arg1	insulation					17:26	Improved thermal insulation	0:26	Improved thermal insulation	0:26	Improved thermal insulation and compressive property of bimodal poly (lactic acid)/cellulose nanocomposite foams.					
36604081	2	22	with	foam	398:401	arg1	structure					423:431	a bimodal cell structure	408:431	a bimodal cell structure	408:431	Only at a specific foaming temperature, PLA/CNF nanocomposites foam with a bimodal cell structure could be obtained.					
36604081	4	23	theme	thermal-insulation	841:858	arg1	property					860:867	excellent thermal-insulation property	831:867	excellent thermal-insulation property	831:867	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	2	24	theme	foaming	354:360	arg1	temperature					362:372	a specific foaming temperature	343:372	a specific foaming temperature	343:372	Only at a specific foaming temperature, PLA/CNF nanocomposites foam with a bimodal cell structure could be obtained.					
36604081	0	25	theme	Improved	0:7	arg1	insulation					17:26	Improved thermal insulation	0:26	Improved thermal insulation	0:26	Improved thermal insulation and compressive property of bimodal poly (lactic acid)/cellulose nanocomposite foams.					
36604081	4	26	theme	excellent	831:839	arg1	property					860:867	excellent thermal-insulation property	831:867	excellent thermal-insulation property	831:867	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	4	27	theme	expansion	718:726	arg1	ratio					728:732	an expansion ratio	715:732	an expansion ratio	715:732	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	2	28	theme	specific	345:352	arg1	temperature					362:372	a specific foaming temperature	343:372	a specific foaming temperature	343:372	Only at a specific foaming temperature, PLA/CNF nanocomposites foam with a bimodal cell structure could be obtained.					
36604081	2	29	theme	bimodal	410:416	arg1	structure					423:431	a bimodal cell structure	408:431	a bimodal cell structure	408:431	Only at a specific foaming temperature, PLA/CNF nanocomposites foam with a bimodal cell structure could be obtained.					
36604081	4	30	theme	0.041 w m-1 k-1	782:796	arg1	times					748:752	20 times	745:752	20 times	745:752	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	4	30	theme	0.041 w m-1 k-1	782:796	arg1	conductivity					766:777	thermal conductivity	758:777	thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property	758:867	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	1	31	theme	bimodal	176:182	arg1	structure					189:197	a bimodal cell structure	174:197	a bimodal cell structure	174:197	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	1	32	theme	supercritical	276:288	arg1	agent					328:332	the foaming agent	316:332	the foaming agent	316:332	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	1	32	theme	supercritical	276:288	arg1	ScCO2					306:310	ScCO2	306:310	ScCO2	306:310	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	1	32	theme	supercritical	276:288	arg1	dioxide					297:303	only supercritical carbon dioxide	271:303	only supercritical carbon dioxide (ScCO2)	271:311	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	1	33	theme	cell	184:187	arg1	structure					189:197	a bimodal cell structure	174:197	a bimodal cell structure	174:197	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	1	34	theme	carbon	290:295	arg1	agent					328:332	the foaming agent	316:332	the foaming agent	316:332	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	1	34	theme	carbon	290:295	arg1	ScCO2					306:310	ScCO2	306:310	ScCO2	306:310	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	1	34	theme	carbon	290:295	arg1	dioxide					297:303	only supercritical carbon dioxide	271:303	only supercritical carbon dioxide (ScCO2)	271:311	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	4	35	theme	PLA/CNF	654:660	arg1	foam					676:679	The prepared PLA/CNF nanocomposite foam	641:679	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure	641:709	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	2	36	theme	nanocomposites	383:396	arg1	foam					398:401	PLA/CNF nanocomposites foam	375:401	PLA/CNF nanocomposites foam with a bimodal cell structure	375:431	Only at a specific foaming temperature, PLA/CNF nanocomposites foam with a bimodal cell structure could be obtained.					
36604081	0	37	theme	compressive	32:42	arg1	property					44:51	compressive property	32:51	compressive property of bimodal poly (lactic acid)	32:81	Improved thermal insulation and compressive property of bimodal poly (lactic acid)/cellulose nanocomposite foams.					
36604081	4	38	theme	cell	696:699	arg1	structure					701:709	a bimodal cell structure	686:709	a bimodal cell structure	686:709	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	5	39	theme	excellent	922:930	arg1	performance					944:954	excellent compression performance	922:954	excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials	922:1058	Meanwhile, the PLA/CNF nanocomposite foam exhibited excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials.					
36604081	2	40	theme	PLA/CNF	375:381	arg1	foam					398:401	PLA/CNF nanocomposites foam	375:401	PLA/CNF nanocomposites foam with a bimodal cell structure	375:431	Only at a specific foaming temperature, PLA/CNF nanocomposites foam with a bimodal cell structure could be obtained.					
36604081	0	41	theme	bimodal	56:62	arg1	poly					64:67	bimodal poly	56:67	bimodal poly (lactic acid)	56:81	Improved thermal insulation and compressive property of bimodal poly (lactic acid)/cellulose nanocomposite foams.					
36604081	0	41	theme	bimodal	56:62	arg1	acid					77:80	lactic acid	70:80	lactic acid	70:80	Improved thermal insulation and compressive property of bimodal poly (lactic acid)/cellulose nanocomposite foams.					
36604081	3	42	theme	cell	625:628	arg1	structure					630:638	the cell structure	621:638	the cell structure	621:638	According to the different crystallization kinetics and nucleation efficiency of samples, it was inferred that the crystallization rate and phase interface would affect the cell structure.					
36604081	4	43	theme	low	815:817	arg1	density					819:825	low density	815:825	low density	815:825	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	3	44	theme	crystallization	567:581	arg1	rate					583:586	the crystallization rate	563:586	the crystallization rate	563:586	According to the different crystallization kinetics and nucleation efficiency of samples, it was inferred that the crystallization rate and phase interface would affect the cell structure.					
36604081	3	45	theme	different	469:477	arg1	kinetics					495:502	the different crystallization kinetics	465:502	the different crystallization kinetics	465:502	According to the different crystallization kinetics and nucleation efficiency of samples, it was inferred that the crystallization rate and phase interface would affect the cell structure.					
36604081	5	46	dep	packaging	1023:1031	arg1	materials					1050:1058	materials	1050:1058	materials	1050:1058	Meanwhile, the PLA/CNF nanocomposite foam exhibited excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials.					
36604081	5	47	theme	compression	932:942	arg1	performance					944:954	excellent compression performance	922:954	excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials	922:1058	Meanwhile, the PLA/CNF nanocomposite foam exhibited excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials.					
36604081	1	48	with	foam	164:167	arg1	structure					189:197	a bimodal cell structure	174:197	a bimodal cell structure	174:197	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36604081	4	49	theme	thermal	758:764	arg1	conductivity					766:777	thermal conductivity	758:777	thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property	758:867	The prepared PLA/CNF nanocomposite foam with a bimodal cell structure had an expansion ratio as high as 20 times and thermal conductivity of 0.041 w m-1 k-1, which exhibited low density and excellent thermal-insulation property.					
36604081	5	50	theme	PLA/CNF	885:891	arg1	foam					907:910	the PLA/CNF nanocomposite foam	881:910	the PLA/CNF nanocomposite foam	881:910	Meanwhile, the PLA/CNF nanocomposite foam exhibited excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials.					
36604081	5	50	theme	PLA/CNF	885:891	arg1	Meanwhile					870:878	Meanwhile	870:878	Meanwhile	870:878	Meanwhile, the PLA/CNF nanocomposite foam exhibited excellent compression performance due to the presence of CNFs, which showed promising application in packaging and construction materials.					
36604081	3	51	theme	phase	592:596	arg1	interface					598:606	phase interface	592:606	phase interface	592:606	According to the different crystallization kinetics and nucleation efficiency of samples, it was inferred that the crystallization rate and phase interface would affect the cell structure.					
36604081	0	52	theme	poly	64:67	arg1	insulation					17:26	Improved thermal insulation	0:26	Improved thermal insulation	0:26	Improved thermal insulation and compressive property of bimodal poly (lactic acid)/cellulose nanocomposite foams.					
36604081	0	52	theme	poly	64:67	arg1	property					44:51	compressive property	32:51	compressive property of bimodal poly (lactic acid)	32:81	Improved thermal insulation and compressive property of bimodal poly (lactic acid)/cellulose nanocomposite foams.					
36604081	3	53	theme	nucleation	508:517	arg1	efficiency					519:528	nucleation efficiency	508:528	nucleation efficiency of samples	508:539	According to the different crystallization kinetics and nucleation efficiency of samples, it was inferred that the crystallization rate and phase interface would affect the cell structure.					
36604081	1	54	theme	simple	216:221	arg1	process					257:263	a simple one-step depressurization foaming process	214:263	a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent	214:332	In this work, an innovative PLA/CNF nanocomposite foam with a bimodal cell structure is prepared by a simple one-step depressurization foaming process using only supercritical carbon dioxide (ScCO2) as the foaming agent.					
36087756	0	0	theme	surimi-wheat	74:85	arg1	dough					87:91	surimi-wheat dough	74:91	surimi-wheat dough	74:91	Effect of konjac glucomannan with different viscosities on the quality of surimi-wheat dough and noodles.					
36087756	1	1	from	concentrations	418:431	arg1	KGM					375:377	KGM	375:377	KGM	375:377	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	1	from	concentrations	418:431	arg1	glucomannan					362:372	konjac glucomannan	355:372	konjac glucomannan (KGM) of different viscosities in different concentrations	355:431	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	3	2	theme	sulfhydryl	609:618	arg1	content					625:631	the free sulfhydryl (SH) content	600:631	the free sulfhydryl (SH) content	600:631	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	1	3	theme	starch-gluten-surimi	145:164	arg1	network					166:172	starch-gluten-surimi network	145:172	starch-gluten-surimi network	145:172	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	4	theme	texture	245:251	arg1	characteristics					253:267	texture characteristics	245:267	texture characteristics	245:267	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	5	from	viscosities	393:403	arg1	concentrations					418:431	different concentrations	408:431	different concentrations	408:431	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	6	theme	konjac	355:360	arg1	KGM					375:377	KGM	375:377	KGM	375:377	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	6	theme	konjac	355:360	arg1	glucomannan					362:372	konjac glucomannan	355:372	konjac glucomannan (KGM) of different viscosities in different concentrations	355:431	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	4	7	theme	tensile	789:795	arg1	length					797:802	tensile length	789:802	tensile length	789:802	The hardness and tensile length of noodles were substantially increased by adding 3 % KGM.					
36087756	6	8	theme	better	1086:1091	arg1	performance					1093:1103	better performance	1086:1103	better performance	1086:1103	More importantly, the application of KGM in the wheat flour composite system also showed better performance.					
36087756	0	9	theme	dough	87:91	arg1	quality					63:69	the quality	59:69	the quality of surimi-wheat dough and noodles	59:103	Effect of konjac glucomannan with different viscosities on the quality of surimi-wheat dough and noodles.					
36087756	5	10	dep	qualities	951:959	arg1	the					939:941	the	939:941	the	939:941	In addition, the KGM enhanced the starch-gluten-surimi network and improved the cooking qualities and textural properties of noodles.					
36087756	3	11	theme	improved	722:729	arg1	enthalpy					731:738	significantly improved enthalpy	708:738	significantly improved enthalpy	708:738	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	0	12	from	viscosities	44:54	arg1	quality					63:69	the quality	59:69	the quality of surimi-wheat dough and noodles	59:103	Effect of konjac glucomannan with different viscosities on the quality of surimi-wheat dough and noodles.					
36087756	0	13	theme	noodles	97:103	arg1	quality					63:69	the quality	59:69	the quality of surimi-wheat dough and noodles	59:103	Effect of konjac glucomannan with different viscosities on the quality of surimi-wheat dough and noodles.					
36087756	3	14	from	increase	656:663	arg1	content					683:689	the free water content	668:689	the free water content	668:689	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	3	14	from	increase	656:663	arg1	content					625:631	the free sulfhydryl (SH) content	600:631	the free sulfhydryl (SH) content	600:631	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	7	15	theme	micro-characteristics	1227:1247	arg1	optimization					1196:1207	the optimization	1192:1207	the optimization of the macro- and micro-characteristics of dough and noodles	1192:1268	Thus, the introduction of KGM into the surimi-wheat dough had a significant effect on the optimization of the macro- and micro-characteristics of dough and noodles.					
36087756	5	16	theme	noodles	988:994	arg1	qualities					951:959	cooking qualities	943:959	cooking qualities	943:959	In addition, the KGM enhanced the starch-gluten-surimi network and improved the cooking qualities and textural properties of noodles.					
36087756	5	16	theme	noodles	988:994	arg1	properties					974:983	textural properties	965:983	textural properties	965:983	In addition, the KGM enhanced the starch-gluten-surimi network and improved the cooking qualities and textural properties of noodles.					
36087756	6	17	theme	composite	1057:1065	arg1	system					1067:1072	the wheat flour composite system	1041:1072	the wheat flour composite system	1041:1072	More importantly, the application of KGM in the wheat flour composite system also showed better performance.					
36087756	7	18	contain	had	1164:1166	arg2	effect					1182:1187	a significant effect	1168:1187	a significant effect	1168:1187	Thus, the introduction of KGM into the surimi-wheat dough had a significant effect on the optimization of the macro- and micro-characteristics of dough and noodles.					
36087756	7	18	contain	had	1164:1166	arg1	introduction					1116:1127	the introduction	1112:1127	the introduction of KGM into the surimi-wheat dough	1112:1162	Thus, the introduction of KGM into the surimi-wheat dough had a significant effect on the optimization of the macro- and micro-characteristics of dough and noodles.					
36087756	1	19	theme	thermal	175:181	arg1	properties					183:192	thermal properties	175:192	thermal properties	175:192	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	20	theme	cooking	270:276	arg1	properties					278:287	cooking properties	270:287	cooking properties	270:287	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	5	21	theme	cooking	943:949	arg1	qualities					951:959	cooking qualities	943:959	cooking qualities	943:959	In addition, the KGM enhanced the starch-gluten-surimi network and improved the cooking qualities and textural properties of noodles.					
36087756	7	22	theme	macro-	1216:1221	arg1	optimization					1196:1207	the optimization	1192:1207	the optimization of the macro- and micro-characteristics of dough and noodles	1192:1268	Thus, the introduction of KGM into the surimi-wheat dough had a significant effect on the optimization of the macro- and micro-characteristics of dough and noodles.					
36087756	1	23	from	glucomannan	362:372	arg1	concentrations					418:431	different concentrations	408:431	different concentrations	408:431	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	7	24	theme	dough	1252:1256	arg1	macro-					1216:1221	macro-	1216:1221	macro-	1216:1221	Thus, the introduction of KGM into the surimi-wheat dough had a significant effect on the optimization of the macro- and micro-characteristics of dough and noodles.					
36087756	7	24	theme	dough	1252:1256	arg1	micro-characteristics					1227:1247	micro-characteristics	1227:1247	micro-characteristics	1227:1247	Thus, the introduction of KGM into the surimi-wheat dough had a significant effect on the optimization of the macro- and micro-characteristics of dough and noodles.					
36087756	6	25	theme	wheat	1045:1049	arg1	system					1067:1072	the wheat flour composite system	1041:1072	the wheat flour composite system	1041:1072	More importantly, the application of KGM in the wheat flour composite system also showed better performance.					
36087756	0	26	theme	konjac	10:15	arg1	Effect					0:5	Effect	0:5	Effect of konjac	0:15	Effect of konjac glucomannan with different viscosities on the quality of surimi-wheat dough and noodles.					
36087756	2	27	theme	G⁎	500:501	arg1	moduli					504:509	complex (G⁎) moduli	491:509	complex (G⁎) moduli	491:509	The results showed that the storage (G'), loss (G″), and complex (G⁎) moduli of dough increased with adding KGM.					
36087756	3	28	theme	KGM	568:570	arg1	viscosity					572:580	KGM viscosity	568:580	KGM viscosity	568:580	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	6	29	theme	flour	1051:1055	arg1	system					1067:1072	the wheat flour composite system	1041:1072	the wheat flour composite system	1041:1072	More importantly, the application of KGM in the wheat flour composite system also showed better performance.					
36087756	0	30	theme	different	34:42	arg1	viscosities					44:54	different viscosities	34:54	different viscosities on the quality of surimi-wheat dough and noodles	34:103	Effect of konjac glucomannan with different viscosities on the quality of surimi-wheat dough and noodles.					
36087756	6	31	theme	KGM	1034:1036	arg1	application					1019:1029	the application	1015:1029	the application of KGM in the wheat flour composite system	1015:1072	More importantly, the application of KGM in the wheat flour composite system also showed better performance.					
36087756	1	32	theme	different	383:391	arg1	viscosities					393:403	different viscosities	383:403	different viscosities in different concentrations	383:431	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	33	theme	water	199:203	arg1	distribution					205:216	water distribution	199:216	water distribution	199:216	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	34	theme	microscopic	294:304	arg1	characteristics					306:320	microscopic characteristics	294:320	microscopic characteristics	294:320	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	2	35	theme	complex	491:497	arg1	moduli					504:509	complex (G⁎) moduli	491:509	complex (G⁎) moduli	491:509	The results showed that the storage (G'), loss (G″), and complex (G⁎) moduli of dough increased with adding KGM.					
36087756	1	36	theme	viscosities	393:403	arg1	KGM					375:377	KGM	375:377	KGM	375:377	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	36	theme	viscosities	393:403	arg1	glucomannan					362:372	konjac glucomannan	355:372	konjac glucomannan (KGM) of different viscosities in different concentrations	355:431	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	7	37	dep	macro-	1216:1221	arg1	the					1212:1214	the	1212:1214	the	1212:1214	Thus, the introduction of KGM into the surimi-wheat dough had a significant effect on the optimization of the macro- and micro-characteristics of dough and noodles.					
36087756	4	38	dep	hardness	776:783	arg1	The					772:774	The	772:774	The	772:774	The hardness and tensile length of noodles were substantially increased by adding 3 % KGM.					
36087756	4	39	theme	%	856:856	arg1	KGM					858:860	3 % KGM	854:860	3 % KGM	854:860	The hardness and tensile length of noodles were substantially increased by adding 3 % KGM.					
36087756	3	40	theme	viscosity	572:580	arg1	increase					556:563	the increase	552:563	the increase of KGM viscosity	552:580	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	6	41	from	application	1019:1029	arg1	system					1067:1072	the wheat flour composite system	1041:1072	the wheat flour composite system	1041:1072	More importantly, the application of KGM in the wheat flour composite system also showed better performance.					
36087756	7	42	theme	significant	1170:1180	arg1	effect					1182:1187	a significant effect	1168:1187	a significant effect	1168:1187	Thus, the introduction of KGM into the surimi-wheat dough had a significant effect on the optimization of the macro- and micro-characteristics of dough and noodles.					
36087756	3	43	theme	free	672:675	arg1	content					683:689	the free water content	668:689	the free water content	668:689	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	2	44	theme	dough	514:518	arg1	loss					476:479	loss	476:479	loss (G″)	476:484	The results showed that the storage (G'), loss (G″), and complex (G⁎) moduli of dough increased with adding KGM.					
36087756	2	44	theme	dough	514:518	arg1	G″					482:483	G″	482:483	G″	482:483	The results showed that the storage (G'), loss (G″), and complex (G⁎) moduli of dough increased with adding KGM.					
36087756	2	44	theme	dough	514:518	arg1	G					471:471	G'	471:472	G'	471:472	The results showed that the storage (G'), loss (G″), and complex (G⁎) moduli of dough increased with adding KGM.					
36087756	2	44	theme	dough	514:518	arg1	moduli					504:509	complex (G⁎) moduli	491:509	complex (G⁎) moduli	491:509	The results showed that the storage (G'), loss (G″), and complex (G⁎) moduli of dough increased with adding KGM.					
36087756	2	44	theme	dough	514:518	arg1	storage					462:468	the storage	458:468	the storage (G')	458:473	The results showed that the storage (G'), loss (G″), and complex (G⁎) moduli of dough increased with adding KGM.					
36087756	1	45	theme	different	408:416	arg1	concentrations					418:431	different concentrations	408:431	different concentrations	408:431	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	3	46	theme	SH	621:622	arg1	content					625:631	the free sulfhydryl (SH) content	600:631	the free sulfhydryl (SH) content	600:631	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	3	47	theme	water	677:681	arg1	content					683:689	the free water content	668:689	the free water content	668:689	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	3	48	theme	free	604:607	arg1	content					625:631	the free sulfhydryl (SH) content	600:631	the free sulfhydryl (SH) content	600:631	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	4	49	theme	noodles	807:813	arg1	hardness					776:783	hardness	776:783	hardness	776:783	The hardness and tensile length of noodles were substantially increased by adding 3 % KGM.					
36087756	4	49	theme	noodles	807:813	arg1	length					797:802	tensile length	789:802	tensile length	789:802	The hardness and tensile length of noodles were substantially increased by adding 3 % KGM.					
36087756	7	50	theme	noodles	1262:1268	arg1	macro-					1216:1221	macro-	1216:1221	macro-	1216:1221	Thus, the introduction of KGM into the surimi-wheat dough had a significant effect on the optimization of the macro- and micro-characteristics of dough and noodles.					
36087756	7	50	theme	noodles	1262:1268	arg1	micro-characteristics					1227:1247	micro-characteristics	1227:1247	micro-characteristics	1227:1247	Thus, the introduction of KGM into the surimi-wheat dough had a significant effect on the optimization of the macro- and micro-characteristics of dough and noodles.					
36087756	1	51	theme	surimi-wheat	221:232	arg1	dough					234:238	surimi-wheat dough	221:238	surimi-wheat dough	221:238	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	3	52	from	reduction	587:595	arg1	content					683:689	the free water content	668:689	the free water content	668:689	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	3	52	from	reduction	587:595	arg1	content					625:631	the free sulfhydryl (SH) content	600:631	the free sulfhydryl (SH) content	600:631	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	5	53	theme	textural	965:972	arg1	properties					974:983	textural properties	965:983	textural properties	965:983	In addition, the KGM enhanced the starch-gluten-surimi network and improved the cooking qualities and textural properties of noodles.					
36087756	7	54	theme	surimi-wheat	1145:1156	arg1	dough					1158:1162	the surimi-wheat dough	1141:1162	the surimi-wheat dough	1141:1162	Thus, the introduction of KGM into the surimi-wheat dough had a significant effect on the optimization of the macro- and micro-characteristics of dough and noodles.					
36087756	3	55	theme	microstructure	748:761	arg1	density					763:769	the microstructure density	744:769	the microstructure density	744:769	With the increase of KGM viscosity, the reduction in the free sulfhydryl (SH) content to 0.84 μmol/g and the increase in the free water content to 8.25 % led to significantly improved enthalpy and the microstructure density.					
36087756	1	56	theme	dough	234:238	arg1	characteristics					306:320	microscopic characteristics	294:320	microscopic characteristics	294:320	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	56	theme	dough	234:238	arg1	properties					183:192	thermal properties	175:192	thermal properties	175:192	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	56	theme	dough	234:238	arg1	rheology					135:142	the rheology	131:142	the rheology	131:142	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	56	theme	dough	234:238	arg1	properties					278:287	cooking properties	270:287	cooking properties	270:287	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	56	theme	dough	234:238	arg1	distribution					205:216	water distribution	199:216	water distribution	199:216	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	56	theme	dough	234:238	arg1	network					166:172	starch-gluten-surimi network	145:172	starch-gluten-surimi network	145:172	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	56	theme	dough	234:238	arg1	characteristics					253:267	texture characteristics	245:267	texture characteristics	245:267	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	7	57	theme	KGM	1132:1134	arg1	introduction					1116:1127	the introduction	1112:1127	the introduction of KGM into the surimi-wheat dough	1112:1162	Thus, the introduction of KGM into the surimi-wheat dough had a significant effect on the optimization of the macro- and micro-characteristics of dough and noodles.					
36087756	1	58	theme	surimi-wheat	329:340	arg1	characteristics					306:320	microscopic characteristics	294:320	microscopic characteristics	294:320	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	58	theme	surimi-wheat	329:340	arg1	properties					183:192	thermal properties	175:192	thermal properties	175:192	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	58	theme	surimi-wheat	329:340	arg1	rheology					135:142	the rheology	131:142	the rheology	131:142	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	58	theme	surimi-wheat	329:340	arg1	properties					278:287	cooking properties	270:287	cooking properties	270:287	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	58	theme	surimi-wheat	329:340	arg1	distribution					205:216	water distribution	199:216	water distribution	199:216	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	58	theme	surimi-wheat	329:340	arg1	network					166:172	starch-gluten-surimi network	145:172	starch-gluten-surimi network	145:172	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	1	58	theme	surimi-wheat	329:340	arg1	characteristics					253:267	texture characteristics	245:267	texture characteristics	245:267	It was investigated that the rheology, starch-gluten-surimi network, thermal properties, and water distribution of surimi-wheat dough, and texture characteristics, cooking properties, and microscopic characteristics of the surimi-wheat noodles with konjac glucomannan (KGM) of different viscosities in different concentrations.					
36087756	5	59	theme	starch-gluten-surimi	897:916	arg1	network					918:924	the starch-gluten-surimi network	893:924	the starch-gluten-surimi network	893:924	In addition, the KGM enhanced the starch-gluten-surimi network and improved the cooking qualities and textural properties of noodles.					
36087756	4	60	theme	3 	854:855	arg1	%					856:856	%	856:856	%	856:856	The hardness and tensile length of noodles were substantially increased by adding 3 % KGM.					
37024646	6	0	dep	uniformity	961:970	arg1	the					957:959	the	957:959	the	957:959	Morphological analysis using a microscope and scanning electron microscope confirmed the uniformity and smoothness of the SLN-ALG film, which improved the mechanical properties of the film.					
37024646	7	1	theme	differential	1106:1117	arg1	calorimetry					1128:1138	differential scanning calorimetry	1106:1138	differential scanning calorimetry	1106:1138	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	1	2	theme	coating	130:136	arg1	film					138:141	a new coating film	124:141	a new coating film for biodegradable polymers and environmentally friendly processing	124:208	The objective of this study was to propose a new coating film for biodegradable polymers and environmentally friendly processing.					
37024646	6	3	theme	Morphological	872:884	arg1	analysis					886:893	Morphological analysis	872:893	Morphological analysis using a microscope and scanning electron microscope	872:945	Morphological analysis using a microscope and scanning electron microscope confirmed the uniformity and smoothness of the SLN-ALG film, which improved the mechanical properties of the film.					
37024646	7	4	theme	scanning	1119:1126	arg1	calorimetry					1128:1138	differential scanning calorimetry	1106:1138	differential scanning calorimetry	1106:1138	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	7	5	theme	film	1195:1198	arg1	formation					1178:1186	the formation	1174:1186	the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer	1174:1319	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	8	6	theme	various	1407:1413	arg1	forms					1438:1442	various enteric-release dosage forms	1407:1442	various enteric-release dosage forms	1407:1442	These promising results suggest a novel nanotechnology-based coating formulation for various enteric-release dosage forms.					
37024646	3	7	theme	coating	535:541	arg1	formulation					543:553	the ALG coating formulation	527:553	the ALG coating formulation	527:553	Experiments were performed on a water-soluble substance (thiamine nitrate) to characterize the effects of SLN upon the addition of the ALG coating formulation.					
37024646	4	8	theme	coated	560:565	arg1	tablets					567:573	The coated tablets	556:573	The coated tablets	556:573	The coated tablets or cast films were characterized based on delayed-release properties, surface morphology, moisture resistance, and chemical interactions.					
37024646	1	9	theme	study	103:107	arg1	objective					85:93	The objective	81:93	The objective of this study	81:107	The objective of this study was to propose a new coating film for biodegradable polymers and environmentally friendly processing.					
37024646	3	10	theme	thiamine	453:460	arg1	substance					442:450	a water-soluble substance	426:450	a water-soluble substance (thiamine nitrate)	426:469	Experiments were performed on a water-soluble substance (thiamine nitrate) to characterize the effects of SLN upon the addition of the ALG coating formulation.					
37024646	3	10	theme	thiamine	453:460	arg1	nitrate					462:468	thiamine nitrate	453:468	thiamine nitrate	453:468	Experiments were performed on a water-soluble substance (thiamine nitrate) to characterize the effects of SLN upon the addition of the ALG coating formulation.					
37024646	8	11	theme	promising	1328:1336	arg1	results					1338:1344	These promising results	1322:1344	These promising results	1322:1344	These promising results suggest a novel nanotechnology-based coating formulation for various enteric-release dosage forms.					
37024646	5	12	theme	intestinal	844:853	arg1	pH					863:864	pH 6.8	863:868	pH 6.8	863:868	The SLN-ALG film displayed gastric-resistant properties (< 10% drug substance dissolved at pH 1.2) and rapid disintegration in the intestinal medium (pH 6.8).					
37024646	5	12	theme	intestinal	844:853	arg1	medium					855:860	the intestinal medium	840:860	the intestinal medium (pH 6.8)	840:869	The SLN-ALG film displayed gastric-resistant properties (< 10% drug substance dissolved at pH 1.2) and rapid disintegration in the intestinal medium (pH 6.8).					
37024646	6	13	theme	film	1056:1059	arg1	properties					1038:1047	the mechanical properties	1023:1047	the mechanical properties of the film	1023:1059	Morphological analysis using a microscope and scanning electron microscope confirmed the uniformity and smoothness of the SLN-ALG film, which improved the mechanical properties of the film.					
37024646	9	14	theme	biodegradability	1462:1477	arg1	ingredients					1493:1503	the proposed ingredients	1480:1503	the proposed ingredients	1480:1503	Because of their biodegradability, the proposed ingredients and processes are safe and environment-friendly.					
37024646	9	14	theme	biodegradability	1462:1477	arg1	safe					1523:1526	safe	1523:1526	safe	1523:1526	Because of their biodegradability, the proposed ingredients and processes are safe and environment-friendly.					
37024646	5	15	dep	substance	781:789	arg1	%					774:774	%	774:774	%	774:774	The SLN-ALG film displayed gastric-resistant properties (< 10% drug substance dissolved at pH 1.2) and rapid disintegration in the intestinal medium (pH 6.8).					
37024646	2	16	theme	nanoparticles	255:267	arg1	implementation					225:238	a novel implementation	217:238	a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition	217:326	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	1	17	theme	biodegradable	147:159	arg1	polymers					161:168	biodegradable polymers	147:168	biodegradable polymers	147:168	The objective of this study was to propose a new coating film for biodegradable polymers and environmentally friendly processing.					
37024646	0	18	theme	Novel	2:6	arg1	Film					17:20	A Novel Alginate Film	0:20	A Novel Alginate Film	0:20	A Novel Alginate Film Based on Nanocoating Approach for Enteric-Release Tablets.					
37024646	7	19	theme	acid	1275:1278	arg1	resistance					1280:1289	a higher acid resistance	1266:1289	a higher acid resistance	1266:1289	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	5	20	theme	gastric-resistant	740:756	arg1	properties					758:767	gastric-resistant properties	740:767	gastric-resistant properties (< 10% drug substance dissolved at pH 1.2)	740:810	The SLN-ALG film displayed gastric-resistant properties (< 10% drug substance dissolved at pH 1.2) and rapid disintegration in the intestinal medium (pH 6.8).					
37024646	2	21	theme	alginate	296:303	arg1	composition					316:326	a biodegradable alginate (ALG) film composition	280:326	a biodegradable alginate (ALG) film composition	280:326	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	4	22	theme	delayed-release	617:631	arg1	properties					633:642	delayed-release properties	617:642	delayed-release properties	617:642	The coated tablets or cast films were characterized based on delayed-release properties, surface morphology, moisture resistance, and chemical interactions.					
37024646	5	23	dep	%	774:774	arg1	10					772:773	10	772:773	10	772:773	The SLN-ALG film displayed gastric-resistant properties (< 10% drug substance dissolved at pH 1.2) and rapid disintegration in the intestinal medium (pH 6.8).					
37024646	2	24	theme	biodegradable	282:294	arg1	composition					316:326	a biodegradable alginate (ALG) film composition	280:326	a biodegradable alginate (ALG) film composition	280:326	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	2	25	theme	gastric-resistant	342:358	arg1	film					360:363	a new gastric-resistant film	336:363	a new gastric-resistant film for an enteric-release tablet	336:393	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	5	26	theme	rapid	816:820	arg1	disintegration					822:835	rapid disintegration	816:835	rapid disintegration	816:835	The SLN-ALG film displayed gastric-resistant properties (< 10% drug substance dissolved at pH 1.2) and rapid disintegration in the intestinal medium (pH 6.8).					
37024646	8	27	theme	novel	1356:1360	arg1	formulation					1391:1401	a novel nanotechnology-based coating formulation	1354:1401	a novel nanotechnology-based coating formulation for various enteric-release dosage forms	1354:1442	These promising results suggest a novel nanotechnology-based coating formulation for various enteric-release dosage forms.					
37024646	8	28	theme	coating	1383:1389	arg1	formulation					1391:1401	a novel nanotechnology-based coating formulation	1354:1401	a novel nanotechnology-based coating formulation for various enteric-release dosage forms	1354:1442	These promising results suggest a novel nanotechnology-based coating formulation for various enteric-release dosage forms.					
37024646	1	29	theme	environmentally	174:188	arg1	processing					199:208	environmentally friendly processing	174:208	environmentally friendly processing	174:208	The objective of this study was to propose a new coating film for biodegradable polymers and environmentally friendly processing.					
37024646	0	30	theme	Alginate	8:15	arg1	Film					17:20	A Novel Alginate Film	0:20	A Novel Alginate Film	0:20	A Novel Alginate Film Based on Nanocoating Approach for Enteric-Release Tablets.					
37024646	3	31	theme	ALG	531:533	arg1	formulation					543:553	the ALG coating formulation	527:553	the ALG coating formulation	527:553	Experiments were performed on a water-soluble substance (thiamine nitrate) to characterize the effects of SLN upon the addition of the ALG coating formulation.					
37024646	1	32	theme	friendly	190:197	arg1	processing					199:208	environmentally friendly processing	174:208	environmentally friendly processing	174:208	The objective of this study was to propose a new coating film for biodegradable polymers and environmentally friendly processing.					
37024646	6	33	theme	electron	927:934	arg1	microscope					936:945	a microscope and scanning electron microscope	901:945	microscope	936:945	Morphological analysis using a microscope and scanning electron microscope confirmed the uniformity and smoothness of the SLN-ALG film, which improved the mechanical properties of the film.					
37024646	3	34	theme	water-soluble	428:440	arg1	substance					442:450	a water-soluble substance	426:450	a water-soluble substance (thiamine nitrate)	426:469	Experiments were performed on a water-soluble substance (thiamine nitrate) to characterize the effects of SLN upon the addition of the ALG coating formulation.					
37024646	3	34	theme	water-soluble	428:440	arg1	nitrate					462:468	thiamine nitrate	453:468	thiamine nitrate	453:468	Experiments were performed on a water-soluble substance (thiamine nitrate) to characterize the effects of SLN upon the addition of the ALG coating formulation.					
37024646	2	35	theme	novel	219:223	arg1	implementation					225:238	a novel implementation	217:238	a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition	217:326	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	4	36	theme	surface	645:651	arg1	morphology					653:662	surface morphology	645:662	surface morphology	645:662	The coated tablets or cast films were characterized based on delayed-release properties, surface morphology, moisture resistance, and chemical interactions.					
37024646	3	37	theme	formulation	543:553	arg1	addition					515:522	the addition	511:522	the addition of the ALG coating formulation	511:553	Experiments were performed on a water-soluble substance (thiamine nitrate) to characterize the effects of SLN upon the addition of the ALG coating formulation.					
37024646	4	38	theme	moisture	665:672	arg1	resistance					674:683	moisture resistance	665:683	moisture resistance	665:683	The coated tablets or cast films were characterized based on delayed-release properties, surface morphology, moisture resistance, and chemical interactions.					
37024646	6	39	theme	scanning	918:925	arg1	microscope					936:945	a microscope and scanning electron microscope	901:945	microscope	936:945	Morphological analysis using a microscope and scanning electron microscope confirmed the uniformity and smoothness of the SLN-ALG film, which improved the mechanical properties of the film.					
37024646	5	40	dep	properties	758:767	arg1	substance					781:789	< 10% drug substance	770:789	< 10% drug substance dissolved at pH 1.2	770:809	The SLN-ALG film displayed gastric-resistant properties (< 10% drug substance dissolved at pH 1.2) and rapid disintegration in the intestinal medium (pH 6.8).					
37024646	8	41	theme	nanotechnology-based	1362:1381	arg1	formulation					1391:1401	a novel nanotechnology-based coating formulation	1354:1401	a novel nanotechnology-based coating formulation for various enteric-release dosage forms	1354:1442	These promising results suggest a novel nanotechnology-based coating formulation for various enteric-release dosage forms.					
37024646	2	42	theme	enteric-release	372:386	arg1	tablet					388:393	an enteric-release tablet	369:393	an enteric-release tablet	369:393	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	5	43	theme	drug	776:779	arg1	substance					781:789	< 10% drug substance	770:789	< 10% drug substance dissolved at pH 1.2	770:809	The SLN-ALG film displayed gastric-resistant properties (< 10% drug substance dissolved at pH 1.2) and rapid disintegration in the intestinal medium (pH 6.8).					
37024646	7	44	dep	Fourier	1062:1068	arg1	transform					1070:1078	transform	1070:1078	transform infrared spectroscopy and differential scanning calorimetry	1070:1138	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	2	45	theme	film	311:314	arg1	composition					316:326	a biodegradable alginate (ALG) film composition	280:326	a biodegradable alginate (ALG) film composition	280:326	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	0	46	theme	Nanocoating	31:41	arg1	Approach					43:50	Nanocoating Approach	31:50	Nanocoating Approach for Enteric-Release Tablets	31:78	A Novel Alginate Film Based on Nanocoating Approach for Enteric-Release Tablets.					
37024646	7	47	theme	free	1218:1221	arg1	groups					1234:1239	free carboxylic groups	1218:1239	free carboxylic groups	1218:1239	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	7	48	theme	infrared	1080:1087	arg1	spectroscopy					1089:1100	infrared spectroscopy	1080:1100	infrared spectroscopy	1080:1100	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	7	49	theme	higher	1268:1273	arg1	resistance					1280:1289	a higher acid resistance	1266:1289	a higher acid resistance	1266:1289	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	4	50	theme	chemical	690:697	arg1	interactions					699:710	chemical interactions	690:710	chemical interactions	690:710	The coated tablets or cast films were characterized based on delayed-release properties, surface morphology, moisture resistance, and chemical interactions.					
37024646	8	51	theme	enteric-release	1415:1429	arg1	forms					1438:1442	various enteric-release dosage forms	1407:1442	various enteric-release dosage forms	1407:1442	These promising results suggest a novel nanotechnology-based coating formulation for various enteric-release dosage forms.					
37024646	2	52	theme	lipid	249:253	arg1	SLN					270:272	SLN	270:272	SLN	270:272	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	2	52	theme	lipid	249:253	arg1	nanoparticles					255:267	solid lipid nanoparticles	243:267	solid lipid nanoparticles (SLN)	243:273	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	2	53	theme	ALG	306:308	arg1	composition					316:326	a biodegradable alginate (ALG) film composition	280:326	a biodegradable alginate (ALG) film composition	280:326	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	7	54	from	pH	1307:1308	arg1	soluble					1296:1302	soluble	1296:1302	soluble	1296:1302	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	4	55	theme	cast	578:581	arg1	films					583:587	cast films	578:587	cast films	578:587	The coated tablets or cast films were characterized based on delayed-release properties, surface morphology, moisture resistance, and chemical interactions.					
37024646	2	56	theme	solid	243:247	arg1	SLN					270:272	SLN	270:272	SLN	270:272	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	2	56	theme	solid	243:247	arg1	nanoparticles					255:267	solid lipid nanoparticles	243:267	solid lipid nanoparticles (SLN)	243:273	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	7	57	from	soluble	1296:1302	arg1	pH					1307:1308	pH 6.8	1307:1312	pH 6.8	1307:1312	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	3	58	theme	SLN	502:504	arg1	effects					491:497	the effects	487:497	the effects of SLN upon the addition of the ALG coating formulation	487:553	Experiments were performed on a water-soluble substance (thiamine nitrate) to characterize the effects of SLN upon the addition of the ALG coating formulation.					
37024646	7	59	theme	soluble	1296:1302	arg1	buffer					1314:1319	soluble in pH 6.8 buffer	1296:1319	soluble in pH 6.8 buffer	1296:1319	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	6	60	theme	film	1002:1005	arg1	smoothness					976:985	smoothness	976:985	smoothness	976:985	Morphological analysis using a microscope and scanning electron microscope confirmed the uniformity and smoothness of the SLN-ALG film, which improved the mechanical properties of the film.					
37024646	6	60	theme	film	1002:1005	arg1	uniformity					961:970	uniformity	961:970	uniformity	961:970	Morphological analysis using a microscope and scanning electron microscope confirmed the uniformity and smoothness of the SLN-ALG film, which improved the mechanical properties of the film.					
37024646	0	61	theme	Enteric-Release	56:70	arg1	Tablets					72:78	Enteric-Release Tablets	56:78	Enteric-Release Tablets	56:78	A Novel Alginate Film Based on Nanocoating Approach for Enteric-Release Tablets.					
37024646	9	62	theme	proposed	1484:1491	arg1	ingredients					1493:1503	the proposed ingredients	1480:1503	the proposed ingredients	1480:1503	Because of their biodegradability, the proposed ingredients and processes are safe and environment-friendly.					
37024646	9	62	theme	proposed	1484:1491	arg1	safe					1523:1526	safe	1523:1526	safe	1523:1526	Because of their biodegradability, the proposed ingredients and processes are safe and environment-friendly.					
37024646	6	63	theme	mechanical	1027:1036	arg1	properties					1038:1047	the mechanical properties	1023:1047	the mechanical properties of the film	1023:1059	Morphological analysis using a microscope and scanning electron microscope confirmed the uniformity and smoothness of the SLN-ALG film, which improved the mechanical properties of the film.					
37024646	2	64	theme	new	338:340	arg1	film					360:363	a new gastric-resistant film	336:363	a new gastric-resistant film for an enteric-release tablet	336:393	Here, a novel implementation of solid lipid nanoparticles (SLN) into a biodegradable alginate (ALG) film composition created a new gastric-resistant film for an enteric-release tablet.					
37024646	6	65	theme	SLN-ALG	994:1000	arg1	film					1002:1005	the SLN-ALG film	990:1005	the SLN-ALG film	990:1005	Morphological analysis using a microscope and scanning electron microscope confirmed the uniformity and smoothness of the SLN-ALG film, which improved the mechanical properties of the film.					
37024646	7	66	theme	carboxylic	1223:1232	arg1	groups					1234:1239	free carboxylic groups	1218:1239	free carboxylic groups	1218:1239	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	8	67	theme	dosage	1431:1436	arg1	forms					1438:1442	various enteric-release dosage forms	1407:1442	various enteric-release dosage forms	1407:1442	These promising results suggest a novel nanotechnology-based coating formulation for various enteric-release dosage forms.					
37024646	7	68	theme	SLN-ALG	1253:1259	arg1	film					1261:1264	the SLN-ALG film	1249:1264	the SLN-ALG film	1249:1264	Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that SLN contributed to the formation of the film, which maintained free carboxylic groups, making the SLN-ALG film a higher acid resistance, but soluble in pH 6.8 buffer.					
37024646	1	69	theme	new	126:128	arg1	film					138:141	a new coating film	124:141	a new coating film for biodegradable polymers and environmentally friendly processing	124:208	The objective of this study was to propose a new coating film for biodegradable polymers and environmentally friendly processing.					
37024646	5	70	theme	SLN-ALG	717:723	arg1	film					725:728	The SLN-ALG film	713:728	The SLN-ALG film	713:728	The SLN-ALG film displayed gastric-resistant properties (< 10% drug substance dissolved at pH 1.2) and rapid disintegration in the intestinal medium (pH 6.8).					
36808039	2	0	theme	elemental	466:474	arg1	composition					476:486	elemental composition	466:486	elemental composition	466:486	The biocomposite was investigated for its physiochemical characteristics such as surface morphology, functional groups, phase determination, and elemental composition.					
36808039	7	1	theme	experimental	1138:1149	arg1	data					1151:1154	the experimental data	1134:1154	the experimental data	1134:1154	Kinetic and isotherm models showed that the experimental data is best fitted to pseudo-second-order kinetic model and the Langmuir isotherm model.					
36808039	9	2	theme	best	1358:1361	arg1	eluent					1363:1368	the best eluent	1354:1368	the best eluent for the extraction of all the three analytes suggested by the extraction study	1354:1447	Ethanol was found to be the best eluent for the extraction of all the three analytes suggested by the extraction study.					
36808039	9	2	theme	best	1358:1361	arg1	Ethanol					1330:1336	Ethanol	1330:1336	Ethanol	1330:1336	Ethanol was found to be the best eluent for the extraction of all the three analytes suggested by the extraction study.					
36808039	1	3	theme	graphene	148:155	arg1	biocomposite					163:174	alginate magnetic graphene oxide biocomposite	130:174	alginate magnetic graphene oxide biocomposite	130:174	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	6	4	theme	optimum	999:1005	arg1	pH = 4					1007:1012	an optimum pH = 4	996:1012	an optimum pH = 4 for aniline = 18.39 mg g-1, for PCA = 17.13 mg g-1, and for PNA = 15.24 mg g-1	996:1091	The maximum adsorption capacities at room temperature have an optimum pH = 4 for aniline = 18.39 mg g-1, for PCA = 17.13 mg g-1, and for PNA = 15.24 mg g-1.					
36808039	0	5	theme	amines	80:85	arg1	removal					60:66	removal	60:66	removal of aromatic amines from aqueous samples	60:106	Alginate magnetic graphene oxide biocomposite synthesis for removal of aromatic amines from aqueous samples.					
36808039	1	6	theme	water	306:310	arg1	samples					312:318	water samples	306:318	water samples	306:318	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	7	theme	oxide	157:161	arg1	biocomposite					163:174	alginate magnetic graphene oxide biocomposite	130:174	alginate magnetic graphene oxide biocomposite	130:174	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	7	8	theme	isotherm	1225:1232	arg1	model					1234:1238	the Langmuir isotherm model	1212:1238	the Langmuir isotherm model	1212:1238	Kinetic and isotherm models showed that the experimental data is best fitted to pseudo-second-order kinetic model and the Langmuir isotherm model.					
36808039	10	9	theme	percent	1462:1468	arg1	recoveries					1470:1479	The maximum percent recoveries	1450:1479	The maximum percent recoveries from spiked water samples	1450:1505	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	0	10	theme	aqueous	92:98	arg1	samples					100:106	aqueous samples	92:106	aqueous samples	92:106	Alginate magnetic graphene oxide biocomposite synthesis for removal of aromatic amines from aqueous samples.					
36808039	10	11	theme	maximum	1454:1460	arg1	recoveries					1470:1479	The maximum percent recoveries	1450:1479	The maximum percent recoveries from spiked water samples	1450:1505	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	10	12	from	processes	1741:1749	arg1	adsorbent					1674:1682	adsorbent	1674:1682	adsorbent	1674:1682	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	3	13	with	biocomposite	580:591	arg1	properties					607:616	magnetic properties	598:616	magnetic properties	598:616	The results revealed that the functional groups of graphene oxide and alginate retained in biocomposite with magnetic properties.					
36808039	7	14	theme	kinetic	1194:1200	arg1	model					1202:1206	pseudo-second-order kinetic model	1174:1206	pseudo-second-order kinetic model	1174:1206	Kinetic and isotherm models showed that the experimental data is best fitted to pseudo-second-order kinetic model and the Langmuir isotherm model.					
36808039	5	15	theme	various	813:819	arg1	conditions					834:843	various experimental conditions	813:843	various experimental conditions like time, pH, concentration, dose, and temperature	813:895	The adsorption process was studied under various experimental conditions like time, pH, concentration, dose, and temperature, and all the parameters were optimized.					
36808039	2	16	theme	physiochemical	363:376	arg1	composition					476:486	elemental composition	466:486	elemental composition	466:486	The biocomposite was investigated for its physiochemical characteristics such as surface morphology, functional groups, phase determination, and elemental composition.					
36808039	2	16	theme	physiochemical	363:376	arg1	morphology					410:419	surface morphology	402:419	surface morphology	402:419	The biocomposite was investigated for its physiochemical characteristics such as surface morphology, functional groups, phase determination, and elemental composition.					
36808039	2	16	theme	physiochemical	363:376	arg1	groups					433:438	functional groups	422:438	functional groups	422:438	The biocomposite was investigated for its physiochemical characteristics such as surface morphology, functional groups, phase determination, and elemental composition.					
36808039	2	16	theme	physiochemical	363:376	arg1	determination					447:459	phase determination	441:459	phase determination	441:459	The biocomposite was investigated for its physiochemical characteristics such as surface morphology, functional groups, phase determination, and elemental composition.					
36808039	2	16	theme	physiochemical	363:376	arg1	characteristics					378:392	its physiochemical characteristics	359:392	its physiochemical characteristics such as surface morphology, functional groups, phase determination, and elemental composition	359:486	The biocomposite was investigated for its physiochemical characteristics such as surface morphology, functional groups, phase determination, and elemental composition.					
36808039	7	17	theme	Langmuir	1216:1223	arg1	model					1234:1238	the Langmuir isotherm model	1212:1238	the Langmuir isotherm model	1212:1238	Kinetic and isotherm models showed that the experimental data is best fitted to pseudo-second-order kinetic model and the Langmuir isotherm model.					
36808039	6	18	from	temperature	979:989	arg1	capacities					960:969	The maximum adsorption capacities	937:969	The maximum adsorption capacities at room temperature	937:989	The maximum adsorption capacities at room temperature have an optimum pH = 4 for aniline = 18.39 mg g-1, for PCA = 17.13 mg g-1, and for PNA = 15.24 mg g-1.					
36808039	0	19	theme	Alginate	0:7	arg1	oxide					27:31	Alginate magnetic graphene oxide	0:31	Alginate magnetic graphene oxide	0:31	Alginate magnetic graphene oxide biocomposite synthesis for removal of aromatic amines from aqueous samples.					
36808039	4	20	theme	water	651:655	arg1	samples					657:663	water samples	651:663	water samples	651:663	The biocomposite was applied to water samples for the removal and extraction of aniline, p-chloroaniline, and p-nitroaniline through adsorption process.					
36808039	0	21	theme	magnetic	9:16	arg1	oxide					27:31	Alginate magnetic graphene oxide	0:31	Alginate magnetic graphene oxide	0:31	Alginate magnetic graphene oxide biocomposite synthesis for removal of aromatic amines from aqueous samples.					
36808039	5	22	theme	adsorption	776:785	arg1	process					787:793	The adsorption process	772:793	The adsorption process	772:793	The adsorption process was studied under various experimental conditions like time, pH, concentration, dose, and temperature, and all the parameters were optimized.					
36808039	10	23	theme	water	1493:1497	arg1	samples					1499:1505	spiked water samples	1486:1505	spiked water samples	1486:1505	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	4	24	theme	p-nitroaniline	729:742	arg1	extraction					685:694	extraction	685:694	extraction	685:694	The biocomposite was applied to water samples for the removal and extraction of aniline, p-chloroaniline, and p-nitroaniline through adsorption process.					
36808039	4	24	theme	p-nitroaniline	729:742	arg1	removal					673:679	removal	673:679	removal	673:679	The biocomposite was applied to water samples for the removal and extraction of aniline, p-chloroaniline, and p-nitroaniline through adsorption process.					
36808039	2	25	theme	functional	422:431	arg1	groups					433:438	functional groups	422:438	functional groups	422:438	The biocomposite was investigated for its physiochemical characteristics such as surface morphology, functional groups, phase determination, and elemental composition.					
36808039	6	26	theme	room	974:977	arg1	temperature					979:989	room temperature	974:989	room temperature	974:989	The maximum adsorption capacities at room temperature have an optimum pH = 4 for aniline = 18.39 mg g-1, for PCA = 17.13 mg g-1, and for PNA = 15.24 mg g-1.					
36808039	7	27	theme	isotherm	1106:1113	arg1	models					1115:1120	Kinetic and isotherm models	1094:1120	models	1115:1120	Kinetic and isotherm models showed that the experimental data is best fitted to pseudo-second-order kinetic model and the Langmuir isotherm model.					
36808039	3	28	theme	alginate	559:566	arg1	groups					530:535	the functional groups	515:535	the functional groups of graphene oxide and alginate	515:566	The results revealed that the functional groups of graphene oxide and alginate retained in biocomposite with magnetic properties.					
36808039	3	28	theme	alginate	559:566	arg1	oxide					549:553	graphene oxide	540:553	graphene oxide	540:553	The results revealed that the functional groups of graphene oxide and alginate retained in biocomposite with magnetic properties.					
36808039	3	28	theme	alginate	559:566	arg1	alginate					559:566	alginate	559:566	alginate	559:566	The results revealed that the functional groups of graphene oxide and alginate retained in biocomposite with magnetic properties.					
36808039	8	29	theme	adsorption	1280:1289	arg1	exothermic					1302:1311	exothermic	1302:1311	exothermic	1302:1311	Thermodynamic study suggested that the adsorption process is exothermic and spontaneous.					
36808039	8	29	theme	adsorption	1280:1289	arg1	process					1291:1297	the adsorption process	1276:1297	the adsorption process	1276:1297	Thermodynamic study suggested that the adsorption process is exothermic and spontaneous.					
36808039	7	30	theme	pseudo-second-order	1174:1192	arg1	model					1202:1206	pseudo-second-order kinetic model	1174:1206	pseudo-second-order kinetic model	1174:1206	Kinetic and isotherm models showed that the experimental data is best fitted to pseudo-second-order kinetic model and the Langmuir isotherm model.					
36808039	5	31	theme	experimental	821:832	arg1	conditions					834:843	various experimental conditions	813:843	various experimental conditions like time, pH, concentration, dose, and temperature	813:895	The adsorption process was studied under various experimental conditions like time, pH, concentration, dose, and temperature, and all the parameters were optimized.					
36808039	3	32	theme	graphene	540:547	arg1	oxide					549:553	graphene oxide	540:553	graphene oxide	540:553	The results revealed that the functional groups of graphene oxide and alginate retained in biocomposite with magnetic properties.					
36808039	10	33	theme	spiked	1486:1491	arg1	samples					1499:1505	spiked water samples	1486:1505	spiked water samples	1486:1505	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	2	34	theme	surface	402:408	arg1	morphology					410:419	surface morphology	402:419	surface morphology	402:419	The biocomposite was investigated for its physiochemical characteristics such as surface morphology, functional groups, phase determination, and elemental composition.					
36808039	0	35	theme	graphene	18:25	arg1	oxide					27:31	Alginate magnetic graphene oxide	0:31	Alginate magnetic graphene oxide	0:31	Alginate magnetic graphene oxide biocomposite synthesis for removal of aromatic amines from aqueous samples.					
36808039	7	36	theme	Kinetic	1094:1100	arg1	models					1115:1120	Kinetic and isotherm models	1094:1120	models	1115:1120	Kinetic and isotherm models showed that the experimental data is best fitted to pseudo-second-order kinetic model and the Langmuir isotherm model.					
36808039	1	37	dep	amines	235:240	arg1	PNA					295:297	PNA	295:297	PNA	295:297	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	37	dep	amines	235:240	arg1	amines					235:240	aromatic amines	226:240	aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA))	226:299	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	37	dep	amines	235:240	arg1	PCA					269:271	PCA	269:271	PCA	269:271	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	37	dep	amines	235:240	arg1	aniline					243:249	aniline	243:249	aniline	243:249	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	37	dep	amines	235:240	arg1	p-chloroaniline					252:266	p-chloroaniline	252:266	p-chloroaniline (PCA)	252:272	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	37	dep	amines	235:240	arg1	p-nitroaniline					279:292	p-nitroaniline	279:292	p-nitroaniline (PNA)	279:298	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	3	38	theme	oxide	549:553	arg1	groups					530:535	the functional groups	515:535	the functional groups of graphene oxide and alginate	515:566	The results revealed that the functional groups of graphene oxide and alginate retained in biocomposite with magnetic properties.					
36808039	3	38	theme	oxide	549:553	arg1	oxide					549:553	graphene oxide	540:553	graphene oxide	540:553	The results revealed that the functional groups of graphene oxide and alginate retained in biocomposite with magnetic properties.					
36808039	3	38	theme	oxide	549:553	arg1	alginate					559:566	alginate	559:566	alginate	559:566	The results revealed that the functional groups of graphene oxide and alginate retained in biocomposite with magnetic properties.					
36808039	10	39	theme	organic	1703:1709	arg1	pollutants					1711:1720	organic pollutants	1703:1720	organic pollutants	1703:1720	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	8	40	theme	Thermodynamic	1241:1253	arg1	study					1255:1259	Thermodynamic study	1241:1259	Thermodynamic study	1241:1259	Thermodynamic study suggested that the adsorption process is exothermic and spontaneous.					
36808039	4	41	theme	aniline	699:705	arg1	extraction					685:694	extraction	685:694	extraction	685:694	The biocomposite was applied to water samples for the removal and extraction of aniline, p-chloroaniline, and p-nitroaniline through adsorption process.					
36808039	4	41	theme	aniline	699:705	arg1	removal					673:679	removal	673:679	removal	673:679	The biocomposite was applied to water samples for the removal and extraction of aniline, p-chloroaniline, and p-nitroaniline through adsorption process.					
36808039	10	42	theme	graphene	1612:1619	arg1	biocomposite					1627:1638	alginate magnetic graphene oxide biocomposite	1594:1638	alginate magnetic graphene oxide biocomposite	1594:1638	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	10	42	theme	graphene	1612:1619	arg1	useful					1651:1656	useful	1651:1656	useful	1651:1656	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	6	43	theme	adsorption	949:958	arg1	capacities					960:969	The maximum adsorption capacities	937:969	The maximum adsorption capacities at room temperature	937:989	The maximum adsorption capacities at room temperature have an optimum pH = 4 for aniline = 18.39 mg g-1, for PCA = 17.13 mg g-1, and for PNA = 15.24 mg g-1.					
36808039	1	44	from	samples	312:318	arg1	removal					200:206	removal	200:206	removal	200:206	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	44	from	samples	312:318	arg1	extraction					212:221	extraction	212:221	extraction	212:221	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	6	45	theme	maximum	941:947	arg1	capacities					960:969	The maximum adsorption capacities	937:969	The maximum adsorption capacities at room temperature	937:989	The maximum adsorption capacities at room temperature have an optimum pH = 4 for aniline = 18.39 mg g-1, for PCA = 17.13 mg g-1, and for PNA = 15.24 mg g-1.					
36808039	4	46	dep	removal	673:679	arg1	the					669:671	the	669:671	the	669:671	The biocomposite was applied to water samples for the removal and extraction of aniline, p-chloroaniline, and p-nitroaniline through adsorption process.					
36808039	10	47	theme	oxide	1621:1625	arg1	biocomposite					1627:1638	alginate magnetic graphene oxide biocomposite	1594:1638	alginate magnetic graphene oxide biocomposite	1594:1638	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	10	47	theme	oxide	1621:1625	arg1	useful					1651:1656	useful	1651:1656	useful	1651:1656	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	9	48	theme	extraction	1432:1441	arg1	study					1443:1447	the extraction study	1428:1447	the extraction study	1428:1447	Ethanol was found to be the best eluent for the extraction of all the three analytes suggested by the extraction study.					
36808039	2	49	theme	phase	441:445	arg1	determination					447:459	phase determination	441:459	phase determination	441:459	The biocomposite was investigated for its physiochemical characteristics such as surface morphology, functional groups, phase determination, and elemental composition.					
36808039	10	50	theme	magnetic	1603:1610	arg1	biocomposite					1627:1638	alginate magnetic graphene oxide biocomposite	1594:1638	alginate magnetic graphene oxide biocomposite	1594:1638	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	10	50	theme	magnetic	1603:1610	arg1	useful					1651:1656	useful	1651:1656	useful	1651:1656	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	6	51	contain	have	991:994	arg1	capacities					960:969	The maximum adsorption capacities	937:969	The maximum adsorption capacities at room temperature	937:989	The maximum adsorption capacities at room temperature have an optimum pH = 4 for aniline = 18.39 mg g-1, for PCA = 17.13 mg g-1, and for PNA = 15.24 mg g-1.					
36808039	6	51	contain	have	991:994	arg2	pH = 4					1007:1012	an optimum pH = 4	996:1012	an optimum pH = 4 for aniline = 18.39 mg g-1, for PCA = 17.13 mg g-1, and for PNA = 15.24 mg g-1	996:1091	The maximum adsorption capacities at room temperature have an optimum pH = 4 for aniline = 18.39 mg g-1, for PCA = 17.13 mg g-1, and for PNA = 15.24 mg g-1.					
36808039	1	52	theme	present	116:122	arg1	work					124:127	the present work	112:127	the present work	112:127	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	10	53	from	samples	1499:1505	arg1	recoveries					1470:1479	The maximum percent recoveries	1450:1479	The maximum percent recoveries from spiked water samples	1450:1505	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	10	54	theme	water	1725:1729	arg1	processes					1741:1749	water treatment processes	1725:1749	water treatment processes	1725:1749	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	4	55	theme	adsorption	752:761	arg1	process					763:769	adsorption process	752:769	adsorption process	752:769	The biocomposite was applied to water samples for the removal and extraction of aniline, p-chloroaniline, and p-nitroaniline through adsorption process.					
36808039	9	56	theme	analytes	1406:1413	arg1	extraction					1378:1387	the extraction	1374:1387	the extraction of all the three analytes suggested by the extraction study	1374:1447	Ethanol was found to be the best eluent for the extraction of all the three analytes suggested by the extraction study.					
36808039	3	57	theme	functional	519:528	arg1	groups					530:535	the functional groups	515:535	the functional groups of graphene oxide and alginate	515:566	The results revealed that the functional groups of graphene oxide and alginate retained in biocomposite with magnetic properties.					
36808039	3	57	theme	functional	519:528	arg1	oxide					549:553	graphene oxide	540:553	graphene oxide	540:553	The results revealed that the functional groups of graphene oxide and alginate retained in biocomposite with magnetic properties.					
36808039	3	57	theme	functional	519:528	arg1	alginate					559:566	alginate	559:566	alginate	559:566	The results revealed that the functional groups of graphene oxide and alginate retained in biocomposite with magnetic properties.					
36808039	0	58	from	samples	100:106	arg1	removal					60:66	removal	60:66	removal of aromatic amines from aqueous samples	60:106	Alginate magnetic graphene oxide biocomposite synthesis for removal of aromatic amines from aqueous samples.					
36808039	10	59	from	adsorbent	1674:1682	arg1	processes					1741:1749	water treatment processes	1725:1749	water treatment processes	1725:1749	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	4	60	theme	p-chloroaniline	708:722	arg1	extraction					685:694	extraction	685:694	extraction	685:694	The biocomposite was applied to water samples for the removal and extraction of aniline, p-chloroaniline, and p-nitroaniline through adsorption process.					
36808039	4	60	theme	p-chloroaniline	708:722	arg1	removal					673:679	removal	673:679	removal	673:679	The biocomposite was applied to water samples for the removal and extraction of aniline, p-chloroaniline, and p-nitroaniline through adsorption process.					
36808039	1	61	theme	aromatic	226:233	arg1	amines					235:240	aromatic amines	226:240	aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA))	226:299	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	61	theme	aromatic	226:233	arg1	p-chloroaniline					252:266	p-chloroaniline	252:266	p-chloroaniline (PCA)	252:272	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	61	theme	aromatic	226:233	arg1	p-nitroaniline					279:292	p-nitroaniline	279:292	p-nitroaniline (PNA)	279:298	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	61	theme	aromatic	226:233	arg1	aniline					243:249	aniline	243:249	aniline	243:249	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	0	62	theme	aromatic	71:78	arg1	amines					80:85	aromatic amines	71:85	aromatic amines	71:85	Alginate magnetic graphene oxide biocomposite synthesis for removal of aromatic amines from aqueous samples.					
36808039	10	63	theme	treatment	1731:1739	arg1	processes					1741:1749	water treatment processes	1725:1749	water treatment processes	1725:1749	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	3	64	theme	magnetic	598:605	arg1	properties					607:616	magnetic properties	598:616	magnetic properties	598:616	The results revealed that the functional groups of graphene oxide and alginate retained in biocomposite with magnetic properties.					
36808039	10	65	theme	pollutants	1711:1720	arg1	removal					1692:1698	the removal	1688:1698	the removal of organic pollutants	1688:1720	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	1	66	theme	alginate	130:137	arg1	biocomposite					163:174	alginate magnetic graphene oxide biocomposite	130:174	alginate magnetic graphene oxide biocomposite	130:174	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	67	theme	amines	235:240	arg1	removal					200:206	removal	200:206	removal	200:206	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	67	theme	amines	235:240	arg1	extraction					212:221	extraction	212:221	extraction	212:221	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	68	dep	removal	200:206	arg1	the					196:198	the	196:198	the	196:198	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	1	69	theme	magnetic	139:146	arg1	biocomposite					163:174	alginate magnetic graphene oxide biocomposite	130:174	alginate magnetic graphene oxide biocomposite	130:174	In the present work, alginate magnetic graphene oxide biocomposite was synthesized for the removal and extraction of aromatic amines (aniline, p-chloroaniline (PCA), and p-nitroaniline (PNA)) from water samples.					
36808039	10	70	theme	alginate	1594:1601	arg1	biocomposite					1627:1638	alginate magnetic graphene oxide biocomposite	1594:1638	alginate magnetic graphene oxide biocomposite	1594:1638	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
36808039	10	70	theme	alginate	1594:1601	arg1	useful					1651:1656	useful	1651:1656	useful	1651:1656	The maximum percent recoveries from spiked water samples were calculated for aniline = 98.82%, PCA = 96.65%, and PNA = 93.55% which showed that alginate magnetic graphene oxide biocomposite could be a useful and ecofriendly adsorbent for the removal of organic pollutants in water treatment processes.					
35861493	4	0	theme	root	929:932	arg1	DRG					944:946	DRG	944:946	DRG	944:946	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	4	0	theme	root	929:932	arg1	ganglion					934:941	the dorsal root ganglion	918:941	the dorsal root ganglion (DRG) neurite	918:955	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	9	1	theme	new	1904:1906	arg1	strategy					1922:1929	a new and effective strategy	1902:1929	a new and effective strategy for the treatment of peripheral nerve injury in the future	1902:1988	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	8	2	theme	focal	1661:1665	arg1	p-FAK					1694:1698	p-FAK	1694:1698	p-FAK	1694:1698	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	8	2	theme	focal	1661:1665	arg1	vinculin					1684:1691	vinculin	1684:1691	vinculin	1684:1691	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	8	2	theme	focal	1661:1665	arg1	protein					1711:1717	Rho A protein	1705:1717	Rho A protein	1705:1717	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	8	2	theme	focal	1661:1665	arg1	markers					1676:1682	the focal adhesion markers	1657:1682	the focal adhesion markers vinculin, p-FAK, and Rho A protein	1657:1717	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	6	3	theme	sciatic	1333:1339	arg1	nerve					1341:1345	sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison	1333:1440	nerve	1341:1345	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	7	4	theme	width	1544:1548	arg1	similar					1564:1570	similar	1564:1570	similar	1564:1570	The elastic modulus of 8.41 kPa and groove width of 30 μm were similar to those of the autograft group.					
35861493	7	4	theme	width	1544:1548	arg1	modulus					1513:1519	The elastic modulus	1501:1519	The elastic modulus of 8.41 kPa and groove width of 30 μm	1501:1557	The elastic modulus of 8.41 kPa and groove width of 30 μm were similar to those of the autograft group.					
35861493	6	5	theme	nerve	1219:1223	arg1	nerve					1212:1216	nerve	1212:1216	nerve	1212:1216	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	6	5	theme	nerve	1219:1223	arg1	examination					1247:1257	nerve electro-physiological examination	1219:1257	nerve electro-physiological examination	1219:1257	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	9	6	theme	effective	1912:1920	arg1	strategy					1922:1929	a new and effective strategy	1902:1929	a new and effective strategy for the treatment of peripheral nerve injury in the future	1902:1988	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	1	7	theme	cues	312:315	arg1	unclear					360:366	unclear	360:366	unclear	360:366	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	1	7	theme	cues	312:315	arg1	effects					296:302	the synergistic effects	280:302	the synergistic effects of both cues on peripheral nerve regeneration	280:348	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	6	8	dep	nerve	1341:1345	arg1	comparison					1431:1440	fiber recovery rate comparison	1411:1440	fiber recovery rate comparison	1411:1440	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	6	8	dep	nerve	1341:1345	arg1	staining					1381:1388	frozen section immunofluorescence staining	1347:1388	frozen section immunofluorescence staining	1347:1388	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	4	9	theme	μm	914:915	arg1	width					902:906	a surface groove width	885:906	a surface groove width of 30 μm	885:915	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	4	9	theme	μm	914:915	arg1	kPa					877:879	5.822 kPa/8.41 kPa	862:879	5.822 kPa/8.41 kPa	862:879	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	6	10	theme	nerve	1212:1216	arg1	observation					1318:1328	transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation	1199:1328	transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison	1199:1440	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	9	11	theme	hydrogel	1813:1820	arg1	implants					1822:1829	our developed hydrogel implants	1799:1829	our developed hydrogel implants containing synergistic cues of elasticity and topographies	1799:1888	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	8	12	theme	axon	1733:1736	arg1	adhesion					1738:1745	axon adhesion	1733:1745	axon adhesion	1733:1745	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	3	13	theme	peripheral	656:665	arg1	regeneration					673:684	peripheral nerve regeneration	656:684	peripheral nerve regeneration	656:684	The physicochemical properties of hydrogels were characterized, and the effect on peripheral nerve regeneration was systematically evaluated via in vitro and in vivo experiments, respectively.					
35861493	4	14	theme	strong	963:968	arg1	ability					977:983	a strong growth ability	961:983	a strong growth ability	961:983	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	6	15	theme	rabbit	1183:1188	arg1	body					1190:1193	the rabbit body	1179:1193	the rabbit body	1179:1193	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	9	16	theme	peripheral	1952:1961	arg1	injury					1969:1974	peripheral nerve injury	1952:1974	peripheral nerve injury in the future	1952:1988	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	6	17	theme	nerve	1405:1409	arg1	observation					1164:1174	General observation	1156:1174	General observation of the rabbit body	1156:1193	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	6	17	theme	nerve	1405:1409	arg1	observation					1318:1328	transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation	1199:1328	transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison	1199:1440	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	5	18	theme	nerve	1100:1104	arg1	defects					1106:1112	rabbit sciatic nerve defects	1085:1112	rabbit sciatic nerve defects	1085:1112	The samples were taken from each group at 2 and 12 weeks after bridging rabbit sciatic nerve defects with a PAM/CS composite hydrogel conduit.					
35861493	6	19	theme	rate	1287:1290	arg1	detection					1292:1300	recovery rate detection	1278:1300	recovery rate detection	1278:1300	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	9	20	theme	injury	1969:1974	arg1	treatment					1939:1947	the treatment	1935:1947	the treatment of peripheral nerve injury in the future	1935:1988	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	4	21	with	hydrogel	826:833	arg1	modulus					851:857	an elastic modulus	840:857	an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm	840:915	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	6	22	theme	immunofluorescence	1362:1379	arg1	staining					1381:1388	frozen section immunofluorescence staining	1347:1388	frozen section immunofluorescence staining	1347:1388	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	6	23	theme	muscle	1260:1265	arg1	weight					1271:1276	muscle wet weight recovery rate detection and comparison	1260:1315	weight	1271:1276	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	6	23	theme	muscle	1260:1265	arg1	nerve					1212:1216	nerve	1212:1216	nerve	1212:1216	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	4	24	theme	PAM/CS	809:814	arg1	hydrogel					826:833	a PAM/CS composite hydrogel	807:833	a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm	807:915	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	3	25	from	effect	646:651	arg1	regeneration					673:684	peripheral nerve regeneration	656:684	peripheral nerve regeneration	656:684	The physicochemical properties of hydrogels were characterized, and the effect on peripheral nerve regeneration was systematically evaluated via in vitro and in vivo experiments, respectively.					
35861493	6	26	theme	frozen	1347:1352	arg1	staining					1381:1388	frozen section immunofluorescence staining	1347:1388	frozen section immunofluorescence staining	1347:1388	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	5	27	theme	rabbit	1085:1090	arg1	nerve					1100:1104	rabbit sciatic nerve	1085:1104	rabbit sciatic nerve defects	1085:1112	The samples were taken from each group at 2 and 12 weeks after bridging rabbit sciatic nerve defects with a PAM/CS composite hydrogel conduit.					
35861493	7	28	theme	elastic	1505:1511	arg1	similar					1564:1570	similar	1564:1570	similar	1564:1570	The elastic modulus of 8.41 kPa and groove width of 30 μm were similar to those of the autograft group.					
35861493	7	28	theme	elastic	1505:1511	arg1	modulus					1513:1519	The elastic modulus	1501:1519	The elastic modulus of 8.41 kPa and groove width of 30 μm	1501:1557	The elastic modulus of 8.41 kPa and groove width of 30 μm were similar to those of the autograft group.					
35861493	6	29	dep	weight	1271:1276	arg1	detection					1292:1300	recovery rate detection	1278:1300	recovery rate detection	1278:1300	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	0	30	theme	synergistic	79:89	arg1	cues					91:94	synergistic cues	79:94	synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration	79:169	Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration.					
35861493	8	31	dep	markers	1676:1682	arg1	p-FAK					1694:1698	p-FAK	1694:1698	p-FAK	1694:1698	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	8	31	dep	markers	1676:1682	arg1	vinculin					1684:1691	vinculin	1684:1691	vinculin	1684:1691	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	8	31	dep	markers	1676:1682	arg1	markers					1676:1682	the focal adhesion markers	1657:1682	the focal adhesion markers vinculin, p-FAK, and Rho A protein	1657:1717	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	8	31	dep	markers	1676:1682	arg1	protein					1711:1717	Rho A protein	1705:1717	Rho A protein	1705:1717	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	7	32	theme	kPa	1529:1531	arg1	similar					1564:1570	similar	1564:1570	similar	1564:1570	The elastic modulus of 8.41 kPa and groove width of 30 μm were similar to those of the autograft group.					
35861493	7	32	theme	kPa	1529:1531	arg1	modulus					1513:1519	The elastic modulus	1501:1519	The elastic modulus of 8.41 kPa and groove width of 30 μm	1501:1557	The elastic modulus of 8.41 kPa and groove width of 30 μm were similar to those of the autograft group.					
35861493	2	33	dep	in	519:520	arg1	situ					522:525	situ	522:525	situ	522:525	In this paper, polyacrylamide/chitosan (PAM/CS) composite hydrogels with synergistic characteristics of elasticity and morphology were prepared using in situ free-radical polymerization and micro-molding.					
35861493	1	34	theme	topographical	197:209	arg1	elasticity					182:191	Substrate elasticity	172:191	Substrate elasticity	172:191	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	1	34	theme	topographical	197:209	arg1	guidance					211:218	topographical guidance	197:218	topographical guidance	197:218	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	1	34	theme	topographical	197:209	arg1	factors					232:238	crucial factors	224:238	crucial factors for regulating tissue regeneration	224:273	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	4	35	theme	better-oriented	989:1003	arg1	status					1005:1010	better-oriented status	989:1010	better-oriented status	989:1010	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	5	36	theme	PAM/CS	1121:1126	arg1	conduit					1147:1153	a PAM/CS composite hydrogel conduit	1119:1153	a PAM/CS composite hydrogel conduit	1119:1153	The samples were taken from each group at 2 and 12 weeks after bridging rabbit sciatic nerve defects with a PAM/CS composite hydrogel conduit.					
35861493	4	37	theme	5.822	862:866	arg1	kPa					877:879	5.822 kPa/8.41 kPa	862:879	5.822 kPa/8.41 kPa	862:879	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	2	38	theme	synergistic	442:452	arg1	characteristics					454:468	synergistic characteristics	442:468	synergistic characteristics of elasticity and morphology	442:497	In this paper, polyacrylamide/chitosan (PAM/CS) composite hydrogels with synergistic characteristics of elasticity and morphology were prepared using in situ free-radical polymerization and micro-molding.					
35861493	6	39	theme	recovery	1417:1424	arg1	comparison					1431:1440	fiber recovery rate comparison	1411:1440	fiber recovery rate comparison	1411:1440	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	5	40	theme	hydrogel	1138:1145	arg1	conduit					1147:1153	a PAM/CS composite hydrogel conduit	1119:1153	a PAM/CS composite hydrogel conduit	1119:1153	The samples were taken from each group at 2 and 12 weeks after bridging rabbit sciatic nerve defects with a PAM/CS composite hydrogel conduit.					
35861493	0	41	theme	hydrogel	51:58	arg1	conduit					60:66	a polyacrylamide/chitosan composite hydrogel conduit	15:66	a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration	15:169	Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration.					
35861493	4	42	dep	in	771:772	arg1	vitro					774:778	vitro	774:778	vitro	774:778	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	9	43	theme	developed	1803:1811	arg1	implants					1822:1829	our developed hydrogel implants	1799:1829	our developed hydrogel implants containing synergistic cues of elasticity and topographies	1799:1888	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	7	44	theme	autograft	1588:1596	arg1	group					1598:1602	the autograft group	1584:1602	the autograft group	1584:1602	The elastic modulus of 8.41 kPa and groove width of 30 μm were similar to those of the autograft group.					
35861493	1	45	theme	nerve	331:335	arg1	regeneration					337:348	peripheral nerve regeneration	320:348	peripheral nerve regeneration	320:348	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	9	46	theme	synergistic	1842:1852	arg1	cues					1854:1857	synergistic cues	1842:1857	synergistic cues of elasticity and topographies	1842:1888	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	4	47	theme	groove	895:900	arg1	width					902:906	a surface groove width	885:906	a surface groove width of 30 μm	885:915	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	0	48	theme	composite	41:49	arg1	conduit					60:66	a polyacrylamide/chitosan composite hydrogel conduit	15:66	a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration	15:169	Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration.					
35861493	6	49	theme	nerve	1478:1482	arg1	transplantation					1484:1498	nerve transplantation	1478:1498	nerve transplantation	1478:1498	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	8	50	theme	same	1612:1615	arg1	time					1617:1620	the same time	1608:1620	the same time	1608:1620	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	3	51	theme	in	732:733	arg1	experiments					740:750	in vitro and in vivo experiments	719:750	in vitro and in vivo experiments	719:750	The physicochemical properties of hydrogels were characterized, and the effect on peripheral nerve regeneration was systematically evaluated via in vitro and in vivo experiments, respectively.					
35861493	0	52	theme	conduit	60:66	arg1	Development					0:10	Development	0:10	Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration.	0:170	Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration.					
35861493	4	53	theme	kPa	877:879	arg1	modulus					851:857	an elastic modulus	840:857	an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm	840:915	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	7	54	theme	μm	1556:1557	arg1	kPa					1529:1531	8.41 kPa	1524:1531	8.41 kPa	1524:1531	The elastic modulus of 8.41 kPa and groove width of 30 μm were similar to those of the autograft group.					
35861493	7	54	theme	μm	1556:1557	arg1	width					1544:1548	groove width	1537:1548	groove width of 30 μm	1537:1557	The elastic modulus of 8.41 kPa and groove width of 30 μm were similar to those of the autograft group.					
35861493	8	55	theme	signaling	1627:1635	arg1	pathways					1637:1644	the signaling pathways	1623:1644	the signaling pathways	1623:1644	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	8	55	theme	signaling	1627:1635	arg1	markers					1676:1682	the focal adhesion markers	1657:1682	the focal adhesion markers vinculin, p-FAK, and Rho A protein	1657:1717	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	2	56	with	hydrogels	427:435	arg1	characteristics					454:468	synergistic characteristics	442:468	synergistic characteristics of elasticity and morphology	442:497	In this paper, polyacrylamide/chitosan (PAM/CS) composite hydrogels with synergistic characteristics of elasticity and morphology were prepared using in situ free-radical polymerization and micro-molding.					
35861493	6	57	theme	nerve	1341:1345	arg1	observation					1164:1174	General observation	1156:1174	General observation of the rabbit body	1156:1193	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	6	57	theme	nerve	1341:1345	arg1	observation					1318:1328	transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation	1199:1328	transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison	1199:1440	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	3	58	theme	physicochemical	578:592	arg1	properties					594:603	The physicochemical properties	574:603	The physicochemical properties of hydrogels	574:616	The physicochemical properties of hydrogels were characterized, and the effect on peripheral nerve regeneration was systematically evaluated via in vitro and in vivo experiments, respectively.					
35861493	7	59	theme	groove	1537:1542	arg1	width					1544:1548	groove width	1537:1548	groove width of 30 μm	1537:1557	The elastic modulus of 8.41 kPa and groove width of 30 μm were similar to those of the autograft group.					
35861493	4	60	theme	dorsal	922:927	arg1	DRG					944:946	DRG	944:946	DRG	944:946	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	4	60	theme	dorsal	922:927	arg1	ganglion					934:941	the dorsal root ganglion	918:941	the dorsal root ganglion (DRG) neurite	918:955	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	0	61	contain	containing	68:77	arg1	conduit					60:66	a polyacrylamide/chitosan composite hydrogel conduit	15:66	a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration	15:169	Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration.					
35861493	0	61	contain	containing	68:77	arg2	cues					91:94	synergistic cues	79:94	synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration	79:169	Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration.					
35861493	8	62	theme	A	1709:1709	arg1	markers					1676:1682	the focal adhesion markers	1657:1682	the focal adhesion markers vinculin, p-FAK, and Rho A protein	1657:1717	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	8	62	theme	A	1709:1709	arg1	protein					1711:1717	Rho A protein	1705:1717	Rho A protein	1705:1717	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	6	63	used	used	1447:1450	arg2	observation					1164:1174	General observation	1156:1174	General observation of the rabbit body	1156:1193	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	6	63	used	used	1447:1450	arg2	observation					1318:1328	transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation	1199:1328	transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison	1199:1440	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	4	64	theme	ganglion	934:941	arg1	neurite					949:955	the dorsal root ganglion (DRG) neurite	918:955	the dorsal root ganglion (DRG) neurite	918:955	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	8	65	theme	adhesion	1667:1674	arg1	p-FAK					1694:1698	p-FAK	1694:1698	p-FAK	1694:1698	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	8	65	theme	adhesion	1667:1674	arg1	vinculin					1684:1691	vinculin	1684:1691	vinculin	1684:1691	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	8	65	theme	adhesion	1667:1674	arg1	protein					1711:1717	Rho A protein	1705:1717	Rho A protein	1705:1717	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	8	65	theme	adhesion	1667:1674	arg1	markers					1676:1682	the focal adhesion markers	1657:1682	the focal adhesion markers vinculin, p-FAK, and Rho A protein	1657:1717	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	9	66	theme	elasticity	1862:1871	arg1	cues					1854:1857	synergistic cues	1842:1857	synergistic cues of elasticity and topographies	1842:1888	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	6	67	theme	transplanted	1199:1210	arg1	observation					1318:1328	transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation	1199:1328	transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison	1199:1440	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	1	68	theme	crucial	224:230	arg1	elasticity					182:191	Substrate elasticity	172:191	Substrate elasticity	172:191	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	1	68	theme	crucial	224:230	arg1	guidance					211:218	topographical guidance	197:218	topographical guidance	197:218	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	1	68	theme	crucial	224:230	arg1	factors					232:238	crucial factors	224:238	crucial factors for regulating tissue regeneration	224:273	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	6	69	theme	body	1190:1193	arg1	observation					1164:1174	General observation	1156:1174	General observation of the rabbit body	1156:1193	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	6	69	theme	body	1190:1193	arg1	observation					1318:1328	transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation	1199:1328	transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison	1199:1440	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	6	70	theme	fiber	1411:1415	arg1	comparison					1431:1440	fiber recovery rate comparison	1411:1440	fiber recovery rate comparison	1411:1440	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	9	71	theme	nerve	1963:1967	arg1	injury					1969:1974	peripheral nerve injury	1952:1974	peripheral nerve injury in the future	1952:1988	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	6	72	theme	myelinated	1394:1403	arg1	nerve					1405:1409	myelinated nerve	1394:1409	myelinated nerve	1394:1409	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	9	73	contain	containing	1831:1840	arg1	implants					1822:1829	our developed hydrogel implants	1799:1829	our developed hydrogel implants containing synergistic cues of elasticity and topographies	1799:1888	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	9	73	contain	containing	1831:1840	arg2	cues					1854:1857	synergistic cues	1842:1857	synergistic cues of elasticity and topographies	1842:1888	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	3	74	dep	in	719:720	arg1	vitro					722:726	vitro	722:726	vitro	722:726	The physicochemical properties of hydrogels were characterized, and the effect on peripheral nerve regeneration was systematically evaluated via in vitro and in vivo experiments, respectively.					
35861493	4	75	theme	growth	970:975	arg1	ability					977:983	a strong growth ability	961:983	a strong growth ability	961:983	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	6	76	theme	recovery	1278:1285	arg1	detection					1292:1300	recovery rate detection	1278:1300	recovery rate detection	1278:1300	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	3	77	theme	nerve	667:671	arg1	regeneration					673:684	peripheral nerve regeneration	656:684	peripheral nerve regeneration	656:684	The physicochemical properties of hydrogels were characterized, and the effect on peripheral nerve regeneration was systematically evaluated via in vitro and in vivo experiments, respectively.					
35861493	0	78	theme	nerve	152:156	arg1	regeneration					158:169	peripheral nerve regeneration	141:169	peripheral nerve regeneration	141:169	Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration.					
35861493	6	79	theme	wet	1267:1269	arg1	weight					1271:1276	muscle wet weight recovery rate detection and comparison	1260:1315	weight	1271:1276	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	6	79	theme	wet	1267:1269	arg1	nerve					1212:1216	nerve	1212:1216	nerve	1212:1216	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	6	80	theme	section	1354:1360	arg1	staining					1381:1388	frozen section immunofluorescence staining	1347:1388	frozen section immunofluorescence staining	1347:1388	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	3	81	theme	in	719:720	arg1	experiments					740:750	in vitro and in vivo experiments	719:750	in vitro and in vivo experiments	719:750	The physicochemical properties of hydrogels were characterized, and the effect on peripheral nerve regeneration was systematically evaluated via in vitro and in vivo experiments, respectively.					
35861493	9	82	from	treatment	1939:1947	arg1	future					1983:1988	future	1983:1988	future	1983:1988	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	5	83	theme	sciatic	1092:1098	arg1	nerve					1100:1104	rabbit sciatic nerve	1085:1104	rabbit sciatic nerve defects	1085:1112	The samples were taken from each group at 2 and 12 weeks after bridging rabbit sciatic nerve defects with a PAM/CS composite hydrogel conduit.					
35861493	1	84	theme	Substrate	172:180	arg1	elasticity					182:191	Substrate elasticity	172:191	Substrate elasticity	172:191	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	1	84	theme	Substrate	172:180	arg1	guidance					211:218	topographical guidance	197:218	topographical guidance	197:218	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	1	84	theme	Substrate	172:180	arg1	factors					232:238	crucial factors	224:238	crucial factors for regulating tissue regeneration	224:273	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	6	85	theme	electro-physiological	1225:1245	arg1	nerve					1212:1216	nerve	1212:1216	nerve	1212:1216	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	6	85	theme	electro-physiological	1225:1245	arg1	examination					1247:1257	nerve electro-physiological examination	1219:1257	nerve electro-physiological examination	1219:1257	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	4	86	theme	in	771:772	arg1	experiments					780:790	The in vitro experiments	767:790	The in vitro experiments	767:790	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	0	87	theme	elasticity	99:108	arg1	cues					91:94	synergistic cues	79:94	synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration	79:169	Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration.					
35861493	0	88	theme	peripheral	141:150	arg1	regeneration					158:169	peripheral nerve regeneration	141:169	peripheral nerve regeneration	141:169	Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration.					
35861493	0	89	theme	topographies	114:125	arg1	cues					91:94	synergistic cues	79:94	synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration	79:169	Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration.					
35861493	4	90	theme	elastic	843:849	arg1	modulus					851:857	an elastic modulus	840:857	an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm	840:915	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	6	91	theme	rate	1426:1429	arg1	comparison					1431:1440	fiber recovery rate comparison	1411:1440	fiber recovery rate comparison	1411:1440	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	5	92	theme	composite	1128:1136	arg1	conduit					1147:1153	a PAM/CS composite hydrogel conduit	1119:1153	a PAM/CS composite hydrogel conduit	1119:1153	The samples were taken from each group at 2 and 12 weeks after bridging rabbit sciatic nerve defects with a PAM/CS composite hydrogel conduit.					
35861493	4	93	contain	had	957:959	arg1	neurite					949:955	the dorsal root ganglion (DRG) neurite	918:955	the dorsal root ganglion (DRG) neurite	918:955	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	4	93	contain	had	957:959	arg2	ability					977:983	a strong growth ability	961:983	a strong growth ability	961:983	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	4	93	contain	had	957:959	arg2	status					1005:1010	better-oriented status	989:1010	better-oriented status	989:1010	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	1	94	from	effects	296:302	arg1	regeneration					337:348	peripheral nerve regeneration	320:348	peripheral nerve regeneration	320:348	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	4	95	theme	composite	816:824	arg1	hydrogel					826:833	a PAM/CS composite hydrogel	807:833	a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm	807:915	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	2	96	theme	composite	417:425	arg1	hydrogels					427:435	polyacrylamide/chitosan (PAM/CS) composite hydrogels	384:435	polyacrylamide/chitosan (PAM/CS) composite hydrogels with synergistic characteristics of elasticity and morphology	384:497	In this paper, polyacrylamide/chitosan (PAM/CS) composite hydrogels with synergistic characteristics of elasticity and morphology were prepared using in situ free-radical polymerization and micro-molding.					
35861493	0	97	theme	polyacrylamide/chitosan	17:39	arg1	conduit					60:66	a polyacrylamide/chitosan composite hydrogel conduit	15:66	a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration	15:169	Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration.					
35861493	1	98	theme	peripheral	320:329	arg1	regeneration					337:348	peripheral nerve regeneration	320:348	peripheral nerve regeneration	320:348	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	9	99	from	future	1983:1988	arg1	treatment					1939:1947	the treatment	1935:1947	the treatment of peripheral nerve injury in the future	1935:1988	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	2	100	theme	morphology	488:497	arg1	characteristics					454:468	synergistic characteristics	442:468	synergistic characteristics of elasticity and morphology	442:497	In this paper, polyacrylamide/chitosan (PAM/CS) composite hydrogels with synergistic characteristics of elasticity and morphology were prepared using in situ free-radical polymerization and micro-molding.					
35861493	2	101	theme	elasticity	473:482	arg1	characteristics					454:468	synergistic characteristics	442:468	synergistic characteristics of elasticity and morphology	442:497	In this paper, polyacrylamide/chitosan (PAM/CS) composite hydrogels with synergistic characteristics of elasticity and morphology were prepared using in situ free-radical polymerization and micro-molding.					
35861493	6	102	theme	transplantation	1484:1498	arg1	effect					1468:1473	the effect	1464:1473	the effect of nerve transplantation	1464:1498	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	1	103	theme	tissue	255:260	arg1	regeneration					262:273	tissue regeneration	255:273	tissue regeneration	255:273	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	2	104	theme	free-radical	527:538	arg1	polymerization					540:553	in situ free-radical polymerization	519:553	in situ free-radical polymerization	519:553	In this paper, polyacrylamide/chitosan (PAM/CS) composite hydrogels with synergistic characteristics of elasticity and morphology were prepared using in situ free-radical polymerization and micro-molding.					
35861493	4	105	theme	surface	887:893	arg1	width					902:906	a surface groove width	885:906	a surface groove width of 30 μm	885:915	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	2	106	theme	in	519:520	arg1	polymerization					540:553	in situ free-radical polymerization	519:553	in situ free-radical polymerization	519:553	In this paper, polyacrylamide/chitosan (PAM/CS) composite hydrogels with synergistic characteristics of elasticity and morphology were prepared using in situ free-radical polymerization and micro-molding.					
35861493	4	107	theme	width	902:906	arg1	modulus					851:857	an elastic modulus	840:857	an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm	840:915	The in vitro experiments showed that on a PAM/CS composite hydrogel with an elastic modulus of 5.822 kPa/8.41 kPa and a surface groove width of 30 μm, the dorsal root ganglion (DRG) neurite had a strong growth ability and better-oriented status.					
35861493	9	108	from	injury	1969:1974	arg1	future					1983:1988	future	1983:1988	future	1983:1988	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	6	109	theme	General	1156:1162	arg1	observation					1164:1174	General observation	1156:1174	General observation of the rabbit body	1156:1193	General observation of the rabbit body and transplanted nerve, nerve electro-physiological examination, muscle wet weight recovery rate detection and comparison, observation of sciatic nerve frozen section immunofluorescence staining and myelinated nerve fiber recovery rate comparison were used to evaluate the effect of nerve transplantation.					
35861493	9	110	theme	topographies	1877:1888	arg1	cues					1854:1857	synergistic cues	1842:1857	synergistic cues of elasticity and topographies	1842:1888	In summary, our developed hydrogel implants containing synergistic cues of elasticity and topographies may provide a new and effective strategy for the treatment of peripheral nerve injury in the future.					
35861493	3	111	dep	in	732:733	arg1	vivo					735:738	vivo	735:738	vivo	735:738	The physicochemical properties of hydrogels were characterized, and the effect on peripheral nerve regeneration was systematically evaluated via in vitro and in vivo experiments, respectively.					
35861493	3	112	theme	hydrogels	608:616	arg1	properties					594:603	The physicochemical properties	574:603	The physicochemical properties of hydrogels	574:616	The physicochemical properties of hydrogels were characterized, and the effect on peripheral nerve regeneration was systematically evaluated via in vitro and in vivo experiments, respectively.					
35861493	8	113	theme	Rho	1705:1707	arg1	markers					1676:1682	the focal adhesion markers	1657:1682	the focal adhesion markers vinculin, p-FAK, and Rho A protein	1657:1717	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	8	113	theme	Rho	1705:1707	arg1	protein					1711:1717	Rho A protein	1705:1717	Rho A protein	1705:1717	At the same time, the signaling pathways, including the focal adhesion markers vinculin, p-FAK, and Rho A protein, referring to axon adhesion and extension, were initially revealed.					
35861493	1	114	theme	synergistic	284:294	arg1	unclear					360:366	unclear	360:366	unclear	360:366	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
35861493	1	114	theme	synergistic	284:294	arg1	effects					296:302	the synergistic effects	280:302	the synergistic effects of both cues on peripheral nerve regeneration	280:348	Substrate elasticity and topographical guidance are crucial factors for regulating tissue regeneration, but the synergistic effects of both cues on peripheral nerve regeneration are still unclear.					
37307973	5	0	theme	good	707:710	arg1	stability					720:728	good thermal stability	707:728	good thermal stability	707:728	The SPI-CHC-rutin (SCR) microcapsules had a gel mesh structure and good thermal stability, and the system was stable and homogeneous after 12 d storage.					
37307973	6	1	theme	intestinal	884:893	arg1	fluids					895:900	simulated gastric and intestinal fluids	862:900	simulated gastric and intestinal fluids	862:900	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	1	2	theme	poor	161:164	arg1	solubility					172:181	poor water solubility	161:181	poor water solubility	161:181	Rutin is a flavonoid polyphenol with excellent biological activity, but due to its instability and poor water solubility, the utilization rate is reduced in vivo.					
37307973	6	3	from	microencapsulation	1143:1160	arg1	bioactivity					1169:1179	the bioactivity	1165:1179	the bioactivity of rutin	1165:1188	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	5	4	theme	thermal	712:718	arg1	stability					720:728	good thermal stability	707:728	good thermal stability	707:728	The SPI-CHC-rutin (SCR) microcapsules had a gel mesh structure and good thermal stability, and the system was stable and homogeneous after 12 d storage.					
37307973	6	5	theme	rutin	1184:1188	arg1	bioactivity					1169:1179	the bioactivity	1165:1179	the bioactivity of rutin	1165:1188	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	2	6	theme	composite	331:339	arg1	coacervation					341:352	composite coacervation	331:352	composite coacervation	331:352	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	2	7	from	hydrochloride	308:320	arg1	Preparation					225:235	Preparation	225:235	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation	225:352	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	2	7	from	hydrochloride	308:320	arg1	microcapsules					246:258	rutin microcapsules	240:258	rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC)	240:326	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	4	8	theme	encapsulation	529:541	arg1	rate					543:546	rutin encapsulation rate	523:546	rutin encapsulation rate	523:546	The rutin encapsulation rate and loading capacity of the microcapsules were 90.34 % and 0.51 % under optimal conditions.					
37307973	6	9	theme	release	824:830	arg1	rates					832:836	the release rates	820:836	the release rates of SCR microcapsules in simulated gastric and intestinal fluids	820:900	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	6	9	theme	release	824:830	arg1	%					913:913	16.97 %	907:913	16.97 %	907:913	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	3	10	theme	SPI	510:512	arg1	%					516:516	SPI 2 %	510:516	SPI 2 %	510:516	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	4	11	theme	loading	552:558	arg1	capacity					560:567	loading capacity	552:567	loading capacity	552:567	The rutin encapsulation rate and loading capacity of the microcapsules were 90.34 % and 0.51 % under optimal conditions.					
37307973	6	12	theme	antioxidant	1059:1069	arg1	activity					1071:1078	superior antioxidant activity	1050:1078	superior antioxidant activity to that of free rutin digests	1050:1108	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	6	13	theme	superior	1050:1057	arg1	activity					1071:1078	superior antioxidant activity	1050:1078	superior antioxidant activity to that of free rutin digests	1050:1108	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	1	14	theme	flavonoid	73:81	arg1	polyphenol					83:92	a flavonoid polyphenol	71:92	a flavonoid polyphenol with excellent biological activity	71:127	Rutin is a flavonoid polyphenol with excellent biological activity, but due to its instability and poor water solubility, the utilization rate is reduced in vivo.					
37307973	1	14	theme	flavonoid	73:81	arg1	Rutin					62:66	Rutin	62:66	Rutin	62:66	Rutin is a flavonoid polyphenol with excellent biological activity, but due to its instability and poor water solubility, the utilization rate is reduced in vivo.					
37307973	5	15	theme	gel	684:686	arg1	structure					693:701	a gel mesh structure	682:701	a gel mesh structure	682:701	The SPI-CHC-rutin (SCR) microcapsules had a gel mesh structure and good thermal stability, and the system was stable and homogeneous after 12 d storage.					
37307973	6	16	theme	targeted	954:961	arg1	release					963:969	a targeted release	952:969	a targeted release of rutin in intestinal fluids	952:999	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	3	17	theme	CHC	502:504	arg1	pH 6					469:472	pH 6	469:472	pH 6	469:472	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	3	17	theme	CHC	502:504	arg1	ratio					447:451	the volume ratio	436:451	the volume ratio of CHC/SPI 1:8	436:466	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	3	17	theme	CHC	502:504	arg1	concentration					485:497	total concentration	479:497	total concentration of CHC and SPI 2 %	479:516	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	6	18	theme	rutin	974:978	arg1	release					963:969	a targeted release	952:969	a targeted release of rutin in intestinal fluids	952:999	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	6	19	theme	intestinal	983:992	arg1	fluids					994:999	intestinal fluids	983:999	intestinal fluids	983:999	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	5	20	theme	mesh	688:691	arg1	structure					693:701	a gel mesh structure	682:701	a gel mesh structure	682:701	The SPI-CHC-rutin (SCR) microcapsules had a gel mesh structure and good thermal stability, and the system was stable and homogeneous after 12 d storage.					
37307973	2	21	theme	protein	273:279	arg1	SPI					290:292	SPI	290:292	SPI	290:292	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	2	21	theme	protein	273:279	arg1	isolate					281:287	soybean protein isolate	265:287	soybean protein isolate (SPI)	265:293	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	0	22	theme	protein	8:14	arg1	complex-rutin					33:45	Soybean protein isolate/chitosan complex-rutin	0:45	Soybean protein isolate/chitosan complex-rutin	0:45	Soybean protein isolate/chitosan complex-rutin microcapsules.					
37307973	5	23	contain	had	678:680	arg2	stability					720:728	good thermal stability	707:728	good thermal stability	707:728	The SPI-CHC-rutin (SCR) microcapsules had a gel mesh structure and good thermal stability, and the system was stable and homogeneous after 12 d storage.					
37307973	5	23	contain	had	678:680	arg2	structure					693:701	a gel mesh structure	682:701	a gel mesh structure	682:701	The SPI-CHC-rutin (SCR) microcapsules had a gel mesh structure and good thermal stability, and the system was stable and homogeneous after 12 d storage.					
37307973	5	23	contain	had	678:680	arg1	microcapsules					664:676	The SPI-CHC-rutin (SCR) microcapsules	640:676	The SPI-CHC-rutin (SCR) microcapsules	640:676	The SPI-CHC-rutin (SCR) microcapsules had a gel mesh structure and good thermal stability, and the system was stable and homogeneous after 12 d storage.					
37307973	6	24	theme	microencapsulation	1143:1160	arg1	protection					1129:1138	a good protection	1122:1138	a good protection of microencapsulation on the bioactivity of rutin	1122:1188	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	1	25	theme	excellent	99:107	arg1	activity					120:127	excellent biological activity	99:127	excellent biological activity	99:127	Rutin is a flavonoid polyphenol with excellent biological activity, but due to its instability and poor water solubility, the utilization rate is reduced in vivo.					
37307973	2	26	from	Preparation	225:235	arg1	CHC					323:325	CHC	323:325	CHC	323:325	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	2	26	from	Preparation	225:235	arg1	SPI					290:292	SPI	290:292	SPI	290:292	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	2	26	from	Preparation	225:235	arg1	isolate					281:287	soybean protein isolate	265:287	soybean protein isolate (SPI)	265:293	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	2	26	from	Preparation	225:235	arg1	hydrochloride					308:320	chitosan hydrochloride	299:320	chitosan hydrochloride (CHC)	299:326	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	4	27	theme	optimal	620:626	arg1	conditions					628:637	optimal conditions	620:637	optimal conditions	620:637	The rutin encapsulation rate and loading capacity of the microcapsules were 90.34 % and 0.51 % under optimal conditions.					
37307973	0	28	theme	Soybean	0:6	arg1	complex-rutin					33:45	Soybean protein isolate/chitosan complex-rutin	0:45	Soybean protein isolate/chitosan complex-rutin	0:45	Soybean protein isolate/chitosan complex-rutin microcapsules.					
37307973	6	29	from	release	963:969	arg1	fluids					994:999	intestinal fluids	983:999	intestinal fluids	983:999	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	2	30	theme	chitosan	299:306	arg1	CHC					323:325	CHC	323:325	CHC	323:325	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	2	30	theme	chitosan	299:306	arg1	hydrochloride					308:320	chitosan hydrochloride	299:320	chitosan hydrochloride (CHC)	299:326	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	1	31	theme	utilization	188:198	arg1	rate					200:203	the utilization rate	184:203	the utilization rate	184:203	Rutin is a flavonoid polyphenol with excellent biological activity, but due to its instability and poor water solubility, the utilization rate is reduced in vivo.					
37307973	8	32	with	compounds	1359:1367	arg1	stability					1398:1406	stability	1398:1406	stability	1398:1406	The present work provides a promising delivery system for natural compounds with low bioavailability and stability.					
37307973	8	32	with	compounds	1359:1367	arg1	bioavailability					1378:1392	low bioavailability	1374:1392	low bioavailability	1374:1392	The present work provides a promising delivery system for natural compounds with low bioavailability and stability.					
37307973	6	33	theme	rutin	1096:1100	arg1	digests					1102:1108	free rutin digests	1091:1108	free rutin digests	1091:1108	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	3	34	theme	%	516:516	arg1	pH 6					469:472	pH 6	469:472	pH 6	469:472	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	3	34	theme	%	516:516	arg1	ratio					447:451	the volume ratio	436:451	the volume ratio of CHC/SPI 1:8	436:466	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	3	34	theme	%	516:516	arg1	concentration					485:497	total concentration	479:497	total concentration of CHC and SPI 2 %	479:516	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	7	35	theme	SCR	1200:1202	arg1	microcapsules					1204:1216	SCR microcapsules	1200:1216	SCR microcapsules developed in this study	1200:1240	Overall, SCR microcapsules developed in this study effectively enhanced the bioavailability of rutin.					
37307973	1	36	theme	biological	109:118	arg1	activity					120:127	excellent biological activity	99:127	excellent biological activity	99:127	Rutin is a flavonoid polyphenol with excellent biological activity, but due to its instability and poor water solubility, the utilization rate is reduced in vivo.					
37307973	4	37	theme	rutin	523:527	arg1	rate					543:546	rutin encapsulation rate	523:546	rutin encapsulation rate	523:546	The rutin encapsulation rate and loading capacity of the microcapsules were 90.34 % and 0.51 % under optimal conditions.					
37307973	6	38	theme	in	800:801	arg1	digestion					809:817	in vitro digestion	800:817	in vitro digestion	800:817	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	1	39	theme	water	166:170	arg1	solubility					172:181	poor water solubility	161:181	poor water solubility	161:181	Rutin is a flavonoid polyphenol with excellent biological activity, but due to its instability and poor water solubility, the utilization rate is reduced in vivo.					
37307973	3	40	theme	CHC/SPI	456:462	arg1	1:8					464:466	CHC/SPI 1:8	456:466	CHC/SPI 1:8	456:466	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	1	41	with	polyphenol	83:92	arg1	activity					120:127	excellent biological activity	99:127	excellent biological activity	99:127	Rutin is a flavonoid polyphenol with excellent biological activity, but due to its instability and poor water solubility, the utilization rate is reduced in vivo.					
37307973	8	42	theme	natural	1351:1357	arg1	compounds					1359:1367	natural compounds	1351:1367	natural compounds with low bioavailability and stability	1351:1406	The present work provides a promising delivery system for natural compounds with low bioavailability and stability.					
37307973	6	43	theme	free	1091:1094	arg1	digests					1102:1108	free rutin digests	1091:1108	free rutin digests	1091:1108	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	3	44	theme	volume	440:445	arg1	ratio					447:451	the volume ratio	436:451	the volume ratio of CHC/SPI 1:8	436:466	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	0	45	theme	isolate/chitosan	16:31	arg1	complex-rutin					33:45	Soybean protein isolate/chitosan complex-rutin	0:45	Soybean protein isolate/chitosan complex-rutin	0:45	Soybean protein isolate/chitosan complex-rutin microcapsules.					
37307973	6	46	theme	gastric	872:878	arg1	fluids					895:900	simulated gastric and intestinal fluids	862:900	simulated gastric and intestinal fluids	862:900	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	4	47	dep	rate	543:546	arg1	The					519:521	The	519:521	The	519:521	The rutin encapsulation rate and loading capacity of the microcapsules were 90.34 % and 0.51 % under optimal conditions.					
37307973	3	48	dep	follows	427:433	arg1	pH 6					469:472	pH 6	469:472	pH 6	469:472	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	3	48	dep	follows	427:433	arg1	ratio					447:451	the volume ratio	436:451	the volume ratio of CHC/SPI 1:8	436:466	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	3	48	dep	follows	427:433	arg1	concentration					485:497	total concentration	479:497	total concentration of CHC and SPI 2 %	479:516	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	5	49	theme	d	782:782	arg1	storage					784:790	12 d storage	779:790	12 d storage	779:790	The SPI-CHC-rutin (SCR) microcapsules had a gel mesh structure and good thermal stability, and the system was stable and homogeneous after 12 d storage.					
37307973	3	50	theme	optimal	388:394	arg1	conditions					408:417	The optimal preparation conditions	384:417	The optimal preparation conditions	384:417	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	6	51	theme	simulated	862:870	arg1	fluids					895:900	simulated gastric and intestinal fluids	862:900	simulated gastric and intestinal fluids	862:900	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	3	52	theme	preparation	396:406	arg1	conditions					408:417	The optimal preparation conditions	384:417	The optimal preparation conditions	384:417	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	6	53	dep	in	800:801	arg1	vitro					803:807	vitro	803:807	vitro	803:807	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	7	54	theme	rutin	1286:1290	arg1	bioavailability					1267:1281	the bioavailability	1263:1281	the bioavailability of rutin	1263:1290	Overall, SCR microcapsules developed in this study effectively enhanced the bioavailability of rutin.					
37307973	3	55	theme	total	479:483	arg1	concentration					485:497	total concentration	479:497	total concentration of CHC and SPI 2 %	479:516	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	8	56	theme	low	1374:1376	arg1	bioavailability					1378:1392	low bioavailability	1374:1392	low bioavailability	1374:1392	The present work provides a promising delivery system for natural compounds with low bioavailability and stability.					
37307973	2	57	from	isolate	281:287	arg1	Preparation					225:235	Preparation	225:235	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation	225:352	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	2	57	from	isolate	281:287	arg1	microcapsules					246:258	rutin microcapsules	240:258	rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC)	240:326	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	6	58	theme	microcapsules	845:857	arg1	rates					832:836	the release rates	820:836	the release rates of SCR microcapsules in simulated gastric and intestinal fluids	820:900	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	6	58	theme	microcapsules	845:857	arg1	%					913:913	16.97 %	907:913	16.97 %	907:913	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	8	59	theme	delivery	1331:1338	arg1	system					1340:1345	a promising delivery system	1319:1345	a promising delivery system for natural compounds with low bioavailability and stability	1319:1406	The present work provides a promising delivery system for natural compounds with low bioavailability and stability.					
37307973	2	60	theme	soybean	265:271	arg1	SPI					290:292	SPI	290:292	SPI	290:292	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	2	60	theme	soybean	265:271	arg1	isolate					281:287	soybean protein isolate	265:287	soybean protein isolate (SPI)	265:293	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	6	61	theme	good	1124:1127	arg1	protection					1129:1138	a good protection	1122:1138	a good protection of microencapsulation on the bioactivity of rutin	1122:1188	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	3	62	theme	1:8	464:466	arg1	pH 6					469:472	pH 6	469:472	pH 6	469:472	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	3	62	theme	1:8	464:466	arg1	ratio					447:451	the volume ratio	436:451	the volume ratio of CHC/SPI 1:8	436:466	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	3	62	theme	1:8	464:466	arg1	concentration					485:497	total concentration	479:497	total concentration of CHC and SPI 2 %	479:516	The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %.					
37307973	5	63	theme	SPI-CHC-rutin	644:656	arg1	microcapsules					664:676	The SPI-CHC-rutin (SCR) microcapsules	640:676	The SPI-CHC-rutin (SCR) microcapsules	640:676	The SPI-CHC-rutin (SCR) microcapsules had a gel mesh structure and good thermal stability, and the system was stable and homogeneous after 12 d storage.					
37307973	8	64	theme	present	1297:1303	arg1	work					1305:1308	The present work	1293:1308	The present work	1293:1308	The present work provides a promising delivery system for natural compounds with low bioavailability and stability.					
37307973	8	65	theme	promising	1321:1329	arg1	system					1340:1345	a promising delivery system	1319:1345	a promising delivery system for natural compounds with low bioavailability and stability	1319:1406	The present work provides a promising delivery system for natural compounds with low bioavailability and stability.					
37307973	6	66	theme	digested	1010:1017	arg1	products					1019:1026	the digested products	1006:1026	the digested products	1006:1026	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	6	67	from	protection	1129:1138	arg1	bioactivity					1169:1179	the bioactivity	1165:1179	the bioactivity of rutin	1165:1188	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	6	68	from	bioactivity	1169:1179	arg1	protection					1129:1138	a good protection	1122:1138	a good protection of microencapsulation on the bioactivity of rutin	1122:1188	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	6	69	from	rates	832:836	arg1	fluids					895:900	simulated gastric and intestinal fluids	862:900	simulated gastric and intestinal fluids	862:900	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	2	70	theme	microcapsules	246:258	arg1	Preparation					225:235	Preparation	225:235	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation	225:352	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
37307973	4	71	theme	microcapsules	576:588	arg1	rate					543:546	rutin encapsulation rate	523:546	rutin encapsulation rate	523:546	The rutin encapsulation rate and loading capacity of the microcapsules were 90.34 % and 0.51 % under optimal conditions.					
37307973	4	71	theme	microcapsules	576:588	arg1	capacity					560:567	loading capacity	552:567	loading capacity	552:567	The rutin encapsulation rate and loading capacity of the microcapsules were 90.34 % and 0.51 % under optimal conditions.					
37307973	6	72	theme	SCR	841:843	arg1	microcapsules					845:857	SCR microcapsules	841:857	SCR microcapsules	841:857	During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin.					
37307973	2	73	theme	rutin	240:244	arg1	microcapsules					246:258	rutin microcapsules	240:258	rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC)	240:326	Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction.					
36807389	8	0	theme	rate-limiting	1087:1099	arg1	properties					1109:1118	the rate-limiting barrier properties	1083:1118	the rate-limiting barrier properties added by the microcapsules	1083:1145	This clearly demonstrates the rate-limiting barrier properties added by the microcapsules, extending the potential functionality from hours to weeks.					
36807389	1	1	theme	sustained	182:190	arg1	release					192:198	sustained release	182:198	sustained release of actives	182:209	Functional textiles is a rapidly growing product segment in which sustained release of actives often plays a key role.					
36807389	5	2	theme	poly	678:681	arg1	microcapsules					709:721	poly(D,L-lactide-co-glycolide) microcapsules	678:721	poly(D,L-lactide-co-glycolide) microcapsules which subsequently are loaded into cellulose nonwovens using a solution blowing technique	678:811	The hydrophobic model substance pyrene is encapsulated in poly(D,L-lactide-co-glycolide) microcapsules which subsequently are loaded into cellulose nonwovens using a solution blowing technique.					
36807389	7	3	theme	encapsulated	1005:1016	arg1	pyrene					1018:1023	encapsulated pyrene	1005:1023	encapsulated pyrene compared to impregnated pyrene	1005:1054	The apparent diffusion coefficient is 100 times lower for encapsulated pyrene compared to impregnated pyrene.					
36807389	8	4	theme	barrier	1101:1107	arg1	properties					1109:1118	the rate-limiting barrier properties	1083:1118	the rate-limiting barrier properties added by the microcapsules	1083:1145	This clearly demonstrates the rate-limiting barrier properties added by the microcapsules, extending the potential functionality from hours to weeks.					
36807389	4	5	from	materials	609:617	arg1	release					588:594	the release	584:594	the release from textile materials	584:617	In this study, microcapsules are presented as a means of extending the release from textile materials.					
36807389	6	6	theme	pyrene	842:847	arg1	release					818:824	The release	814:824	The release of encapsulated pyrene	814:847	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	7	7	dep	times	989:993	arg1	lower					995:999	lower	995:999	lower	995:999	The apparent diffusion coefficient is 100 times lower for encapsulated pyrene compared to impregnated pyrene.					
36807389	2	8	theme	resource	326:333	arg1	loss					300:303	premature loss	290:303	premature loss of functionality and resource inefficiency	290:346	Failure to sustain the release results in costs due to premature loss of functionality and resource inefficiency.					
36807389	0	9	from	nonwovens	79:87	arg1	release					56:62	rate-determining release	39:62	rate-determining release from cellulose nonwovens for long-term performance	39:113	Microcapsule functionalization enables rate-determining release from cellulose nonwovens for long-term performance.					
36807389	2	10	theme	premature	290:298	arg1	loss					300:303	premature loss	290:303	premature loss of functionality and resource inefficiency	290:346	Failure to sustain the release results in costs due to premature loss of functionality and resource inefficiency.					
36807389	5	11	theme	hydrophobic	624:634	arg1	pyrene					652:657	The hydrophobic model substance pyrene	620:657	The hydrophobic model substance pyrene	620:657	The hydrophobic model substance pyrene is encapsulated in poly(D,L-lactide-co-glycolide) microcapsules which subsequently are loaded into cellulose nonwovens using a solution blowing technique.					
36807389	0	12	theme	Microcapsule	0:11	arg1	functionalization					13:29	Microcapsule functionalization	0:29	Microcapsule functionalization	0:29	Microcapsule functionalization enables rate-determining release from cellulose nonwovens for long-term performance.					
36807389	4	13	theme	textile	601:607	arg1	materials					609:617	textile materials	601:617	textile materials	601:617	In this study, microcapsules are presented as a means of extending the release from textile materials.					
36807389	6	14	theme	bulk	928:931	arg1	impregnation					933:944	bulk impregnation	928:944	bulk impregnation	928:944	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	6	14	theme	bulk	928:931	arg1	methods					907:913	two conventional functionalization methods	872:913	two conventional functionalization methods: surface and bulk impregnation	872:944	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	5	15	theme	substance	642:650	arg1	pyrene					652:657	The hydrophobic model substance pyrene	620:657	The hydrophobic model substance pyrene	620:657	The hydrophobic model substance pyrene is encapsulated in poly(D,L-lactide-co-glycolide) microcapsules which subsequently are loaded into cellulose nonwovens using a solution blowing technique.					
36807389	2	16	dep	Failure	235:241	arg1	sustain					246:252	sustain	246:252	to sustain the release	243:264	Failure to sustain the release results in costs due to premature loss of functionality and resource inefficiency.					
36807389	0	17	theme	cellulose	69:77	arg1	nonwovens					79:87	cellulose nonwovens	69:87	cellulose nonwovens	69:87	Microcapsule functionalization enables rate-determining release from cellulose nonwovens for long-term performance.					
36807389	7	18	theme	100	985:987	arg1	coefficient					970:980	The apparent diffusion coefficient	947:980	The apparent diffusion coefficient	947:980	The apparent diffusion coefficient is 100 times lower for encapsulated pyrene compared to impregnated pyrene.					
36807389	7	18	theme	100	985:987	arg1	times					989:993	times	989:993	times	989:993	The apparent diffusion coefficient is 100 times lower for encapsulated pyrene compared to impregnated pyrene.					
36807389	2	19	theme	due	283:285	arg1	costs					277:281	costs	277:281	costs due to premature loss of functionality and resource inefficiency	277:346	Failure to sustain the release results in costs due to premature loss of functionality and resource inefficiency.					
36807389	3	20	theme	biocidal	462:469	arg1	actives					471:477	biocidal actives	462:477	biocidal actives	462:477	Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution.					
36807389	0	21	theme	long-term	93:101	arg1	performance					103:113	long-term performance	93:113	long-term performance	93:113	Microcapsule functionalization enables rate-determining release from cellulose nonwovens for long-term performance.					
36807389	1	22	theme	Functional	116:125	arg1	textiles					127:134	Functional textiles	116:134	Functional textiles	116:134	Functional textiles is a rapidly growing product segment in which sustained release of actives often plays a key role.					
36807389	1	22	theme	Functional	116:125	arg1	segment					165:171	a rapidly growing product segment	139:171	a rapidly growing product segment in which sustained release of actives often plays a key role	139:232	Functional textiles is a rapidly growing product segment in which sustained release of actives often plays a key role.					
36807389	3	23	theme	excessive	414:422	arg1	release					441:447	an excessive and uncontrolled release	411:447	an excessive and uncontrolled release	411:447	Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution.					
36807389	6	24	dep	that	864:867	arg1	is					849:850	is	849:850	is compared to that of two conventional functionalization methods: surface and bulk impregnation	849:944	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	0	25	theme	rate-determining	39:54	arg1	release					56:62	rate-determining release	39:62	rate-determining release from cellulose nonwovens for long-term performance	39:113	Microcapsule functionalization enables rate-determining release from cellulose nonwovens for long-term performance.					
36807389	5	26	theme	model	636:640	arg1	pyrene					652:657	The hydrophobic model substance pyrene	620:657	The hydrophobic model substance pyrene	620:657	The hydrophobic model substance pyrene is encapsulated in poly(D,L-lactide-co-glycolide) microcapsules which subsequently are loaded into cellulose nonwovens using a solution blowing technique.					
36807389	2	27	theme	functionality	308:320	arg1	loss					300:303	premature loss	290:303	premature loss of functionality and resource inefficiency	290:346	Failure to sustain the release results in costs due to premature loss of functionality and resource inefficiency.					
36807389	5	28	dep	poly	678:681	arg1	L-lactide-co-glycolide					685:706	L-lactide-co-glycolide	685:706	L-lactide-co-glycolide	685:706	The hydrophobic model substance pyrene is encapsulated in poly(D,L-lactide-co-glycolide) microcapsules which subsequently are loaded into cellulose nonwovens using a solution blowing technique.					
36807389	5	28	dep	poly	678:681	arg1	D					683:683	D	683:683	D	683:683	The hydrophobic model substance pyrene is encapsulated in poly(D,L-lactide-co-glycolide) microcapsules which subsequently are loaded into cellulose nonwovens using a solution blowing technique.					
36807389	1	29	theme	key	225:227	arg1	role					229:232	a key role	223:232	a key role	223:232	Functional textiles is a rapidly growing product segment in which sustained release of actives often plays a key role.					
36807389	8	30	theme	potential	1162:1170	arg1	functionality					1172:1184	the potential functionality	1158:1184	the potential functionality	1158:1184	This clearly demonstrates the rate-limiting barrier properties added by the microcapsules, extending the potential functionality from hours to weeks.					
36807389	6	31	theme	functionalization	889:905	arg1	surface					916:922	surface	916:922	surface	916:922	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	6	31	theme	functionalization	889:905	arg1	impregnation					933:944	bulk impregnation	928:944	bulk impregnation	928:944	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	6	31	theme	functionalization	889:905	arg1	methods					907:913	two conventional functionalization methods	872:913	two conventional functionalization methods: surface and bulk impregnation	872:944	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	3	32	dep	-	456:456	arg1	release					441:447	an excessive and uncontrolled release	411:447	an excessive and uncontrolled release	411:447	Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution.					
36807389	3	32	dep	-	456:456	arg1	results					481:487	results	481:487	results	481:487	Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution.					
36807389	3	32	dep	-	456:456	arg1	actives					471:477	biocidal actives	462:477	biocidal actives	462:477	Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution.					
36807389	3	33	from	actives	471:477	arg1	pollution					506:514	environmental pollution	492:514	environmental pollution	492:514	Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution.					
36807389	6	34	theme	conventional	876:887	arg1	surface					916:922	surface	916:922	surface	916:922	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	6	34	theme	conventional	876:887	arg1	impregnation					933:944	bulk impregnation	928:944	bulk impregnation	928:944	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	6	34	theme	conventional	876:887	arg1	methods					907:913	two conventional functionalization methods	872:913	two conventional functionalization methods: surface and bulk impregnation	872:944	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	2	35	dep	functionality	308:320	arg1	inefficiency					335:346	inefficiency	335:346	inefficiency	335:346	Failure to sustain the release results in costs due to premature loss of functionality and resource inefficiency.					
36807389	3	36	theme	uncontrolled	428:439	arg1	release					441:447	an excessive and uncontrolled release	411:447	an excessive and uncontrolled release	411:447	Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution.					
36807389	1	37	theme	growing	149:155	arg1	textiles					127:134	Functional textiles	116:134	Functional textiles	116:134	Functional textiles is a rapidly growing product segment in which sustained release of actives often plays a key role.					
36807389	1	37	theme	growing	149:155	arg1	segment					165:171	a rapidly growing product segment	139:171	a rapidly growing product segment in which sustained release of actives often plays a key role	139:232	Functional textiles is a rapidly growing product segment in which sustained release of actives often plays a key role.					
36807389	7	38	theme	apparent	951:958	arg1	coefficient					970:980	The apparent diffusion coefficient	947:980	The apparent diffusion coefficient	947:980	The apparent diffusion coefficient is 100 times lower for encapsulated pyrene compared to impregnated pyrene.					
36807389	7	38	theme	apparent	951:958	arg1	times					989:993	times	989:993	times	989:993	The apparent diffusion coefficient is 100 times lower for encapsulated pyrene compared to impregnated pyrene.					
36807389	6	39	dep	methods	907:913	arg1	surface					916:922	surface	916:922	surface	916:922	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	6	39	dep	methods	907:913	arg1	impregnation					933:944	bulk impregnation	928:944	bulk impregnation	928:944	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	6	39	dep	methods	907:913	arg1	methods					907:913	two conventional functionalization methods	872:913	two conventional functionalization methods: surface and bulk impregnation	872:944	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	1	40	theme	product	157:163	arg1	textiles					127:134	Functional textiles	116:134	Functional textiles	116:134	Functional textiles is a rapidly growing product segment in which sustained release of actives often plays a key role.					
36807389	1	40	theme	product	157:163	arg1	segment					165:171	a rapidly growing product segment	139:171	a rapidly growing product segment in which sustained release of actives often plays a key role	139:232	Functional textiles is a rapidly growing product segment in which sustained release of actives often plays a key role.					
36807389	7	41	theme	diffusion	960:968	arg1	coefficient					970:980	The apparent diffusion coefficient	947:980	The apparent diffusion coefficient	947:980	The apparent diffusion coefficient is 100 times lower for encapsulated pyrene compared to impregnated pyrene.					
36807389	7	41	theme	diffusion	960:968	arg1	times					989:993	times	989:993	times	989:993	The apparent diffusion coefficient is 100 times lower for encapsulated pyrene compared to impregnated pyrene.					
36807389	7	42	theme	impregnated	1037:1047	arg1	pyrene					1049:1054	impregnated pyrene	1037:1054	impregnated pyrene	1037:1054	The apparent diffusion coefficient is 100 times lower for encapsulated pyrene compared to impregnated pyrene.					
36807389	6	43	theme	encapsulated	829:840	arg1	pyrene					842:847	encapsulated pyrene	829:847	encapsulated pyrene	829:847	The release of encapsulated pyrene is compared to that of two conventional functionalization methods: surface and bulk impregnation.					
36807389	3	44	theme	environmental	492:504	arg1	pollution					506:514	environmental pollution	492:514	environmental pollution	492:514	Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution.					
36807389	3	45	theme	Conventional	349:360	arg1	methods					374:380	Conventional application methods	349:380	Conventional application methods such as impregnation	349:401	Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution.					
36807389	3	45	theme	Conventional	349:360	arg1	impregnation					390:401	impregnation	390:401	impregnation	390:401	Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution.					
36807389	1	46	theme	actives	203:209	arg1	release					192:198	sustained release	182:198	sustained release of actives	182:209	Functional textiles is a rapidly growing product segment in which sustained release of actives often plays a key role.					
36807389	3	47	theme	application	362:372	arg1	methods					374:380	Conventional application methods	349:380	Conventional application methods such as impregnation	349:401	Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution.					
36807389	3	47	theme	application	362:372	arg1	impregnation					390:401	impregnation	390:401	impregnation	390:401	Conventional application methods such as impregnation lead to an excessive and uncontrolled release, which - for biocidal actives - results in environmental pollution.					
36807389	5	48	theme	cellulose	758:766	arg1	nonwovens					768:776	cellulose nonwovens	758:776	cellulose nonwovens using a solution blowing technique	758:811	The hydrophobic model substance pyrene is encapsulated in poly(D,L-lactide-co-glycolide) microcapsules which subsequently are loaded into cellulose nonwovens using a solution blowing technique.					
36302486	4	0	theme	blood	773:777	arg1	loss					779:782	blood loss	773:782	blood loss	773:782	The thermosensitive hydrogels significantly reduce whole blood coagulation time in vitro and both hemostasis time and blood loss in vivo using rat hepatic hemorrhage and tail amputation models.					
36302486	5	1	theme	cell	915:918	arg1	viability					920:928	cell viability	915:928	cell viability	915:928	These improvements are biocompatible, without adversely affecting cell viability, blood components, biodegradability, or introducing notable inflammation, thus enabling injury healing.					
36302486	3	2	theme	adhesive	556:563	arg1	strength					565:572	its tissue adhesive strength	545:572	its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported	545:652	This was found to decrease gelation time by >2 times around 37 °C while increasing hydrogel internal network structure, with its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported.					
36302486	6	3	theme	facile	1085:1090	arg1	approach					1092:1099	a facile approach	1083:1099	a facile approach to enhance thermogelling and bioadhesion of chitosan-based hydrogels via noncovalent supramolecular mechanisms	1083:1210	Moreover, our results displayed the potential of a facile approach to enhance thermogelling and bioadhesion of chitosan-based hydrogels via noncovalent supramolecular mechanisms.					
36302486	6	4	theme	chitosan-based	1145:1158	arg1	hydrogels					1160:1168	chitosan-based hydrogels	1145:1168	chitosan-based hydrogels	1145:1168	Moreover, our results displayed the potential of a facile approach to enhance thermogelling and bioadhesion of chitosan-based hydrogels via noncovalent supramolecular mechanisms.					
36302486	5	5	theme	notable	982:988	arg1	inflammation					990:1001	introducing notable inflammation	970:1001	introducing notable inflammation	970:1001	These improvements are biocompatible, without adversely affecting cell viability, blood components, biodegradability, or introducing notable inflammation, thus enabling injury healing.					
36302486	5	6	theme	introducing	970:980	arg1	inflammation					990:1001	introducing notable inflammation	970:1001	introducing notable inflammation	970:1001	These improvements are biocompatible, without adversely affecting cell viability, blood components, biodegradability, or introducing notable inflammation, thus enabling injury healing.					
36302486	2	7	theme	tissue	355:360	arg1	adherence					362:370	wet tissue adherence	351:370	wet tissue adherence	351:370	Herein, we report an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA).					
36302486	4	8	theme	hemorrhage	810:819	arg1	models					841:846	rat hepatic hemorrhage and tail amputation models	798:846	rat hepatic hemorrhage and tail amputation models	798:846	The thermosensitive hydrogels significantly reduce whole blood coagulation time in vitro and both hemostasis time and blood loss in vivo using rat hepatic hemorrhage and tail amputation models.					
36302486	4	9	theme	hepatic	802:808	arg1	hemorrhage					810:819	rat hepatic hemorrhage	798:819	rat hepatic hemorrhage	798:819	The thermosensitive hydrogels significantly reduce whole blood coagulation time in vitro and both hemostasis time and blood loss in vivo using rat hepatic hemorrhage and tail amputation models.					
36302486	4	10	theme	tail	825:828	arg1	amputation					830:839	tail amputation	825:839	tail amputation	825:839	The thermosensitive hydrogels significantly reduce whole blood coagulation time in vitro and both hemostasis time and blood loss in vivo using rat hepatic hemorrhage and tail amputation models.					
36302486	2	11	theme	wet	351:353	arg1	adherence					362:370	wet tissue adherence	351:370	wet tissue adherence	351:370	Herein, we report an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA).					
36302486	1	12	theme	shaped	172:177	arg1	wounds					188:193	irregularly shaped bleeding wounds	160:193	irregularly shaped bleeding wounds	160:193	Development of an injectable hemostatic for treating noncompressible or irregularly shaped bleeding wounds remains a pressing medical need.					
36302486	4	13	theme	whole	706:710	arg1	time					730:733	whole blood coagulation time	706:733	whole blood coagulation time in vitro	706:742	The thermosensitive hydrogels significantly reduce whole blood coagulation time in vitro and both hemostasis time and blood loss in vivo using rat hepatic hemorrhage and tail amputation models.					
36302486	3	14	theme	greater	583:589	arg1	strength					565:572	its tissue adhesive strength	545:572	its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported	545:652	This was found to decrease gelation time by >2 times around 37 °C while increasing hydrogel internal network structure, with its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported.					
36302486	1	15	theme	pressing	205:212	arg1	need					222:225	a pressing medical need	203:225	a pressing medical need	203:225	Development of an injectable hemostatic for treating noncompressible or irregularly shaped bleeding wounds remains a pressing medical need.					
36302486	1	16	theme	medical	214:220	arg1	need					222:225	a pressing medical need	203:225	a pressing medical need	203:225	Development of an injectable hemostatic for treating noncompressible or irregularly shaped bleeding wounds remains a pressing medical need.					
36302486	1	17	theme	injectable	106:115	arg1	hemostatic					117:126	an injectable hemostatic	103:126	an injectable hemostatic for treating noncompressible or irregularly shaped bleeding wounds	103:193	Development of an injectable hemostatic for treating noncompressible or irregularly shaped bleeding wounds remains a pressing medical need.					
36302486	3	18	theme	gelation	447:454	arg1	time					456:459	gelation time	447:459	gelation time	447:459	This was found to decrease gelation time by >2 times around 37 °C while increasing hydrogel internal network structure, with its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported.					
36302486	1	19	theme	hemostatic	117:126	arg1	Development					88:98	Development	88:98	Development of an injectable hemostatic for treating noncompressible or irregularly shaped bleeding wounds	88:193	Development of an injectable hemostatic for treating noncompressible or irregularly shaped bleeding wounds remains a pressing medical need.					
36302486	6	20	theme	hydrogels	1160:1168	arg1	bioadhesion					1130:1140	bioadhesion	1130:1140	bioadhesion	1130:1140	Moreover, our results displayed the potential of a facile approach to enhance thermogelling and bioadhesion of chitosan-based hydrogels via noncovalent supramolecular mechanisms.					
36302486	6	20	theme	hydrogels	1160:1168	arg1	thermogelling					1112:1124	thermogelling	1112:1124	thermogelling	1112:1124	Moreover, our results displayed the potential of a facile approach to enhance thermogelling and bioadhesion of chitosan-based hydrogels via noncovalent supramolecular mechanisms.					
36302486	0	21	theme	chitosan-based	37:50	arg1	hydrogels					52:60	bioadhesive chitosan-based hydrogels	25:60	bioadhesive chitosan-based hydrogels for efficient hemostasis	25:85	Injectable thermogelling bioadhesive chitosan-based hydrogels for efficient hemostasis.					
36302486	4	22	theme	rat	798:800	arg1	hemorrhage					810:819	rat hepatic hemorrhage	798:819	rat hepatic hemorrhage	798:819	The thermosensitive hydrogels significantly reduce whole blood coagulation time in vitro and both hemostasis time and blood loss in vivo using rat hepatic hemorrhage and tail amputation models.					
36302486	5	23	theme	injury	1018:1023	arg1	healing					1025:1031	injury healing	1018:1031	injury healing	1018:1031	These improvements are biocompatible, without adversely affecting cell viability, blood components, biodegradability, or introducing notable inflammation, thus enabling injury healing.					
36302486	3	24	theme	network	521:527	arg1	structure					529:537	hydrogel internal network structure	503:537	hydrogel internal network structure	503:537	This was found to decrease gelation time by >2 times around 37 °C while increasing hydrogel internal network structure, with its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported.					
36302486	0	25	theme	bioadhesive	25:35	arg1	hydrogels					52:60	bioadhesive chitosan-based hydrogels	25:60	bioadhesive chitosan-based hydrogels for efficient hemostasis	25:85	Injectable thermogelling bioadhesive chitosan-based hydrogels for efficient hemostasis.					
36302486	3	26	theme	non-composite	608:620	arg1	hydrogel					622:629	the non-composite hydrogel	604:629	the non-composite hydrogel	604:629	This was found to decrease gelation time by >2 times around 37 °C while increasing hydrogel internal network structure, with its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported.					
36302486	1	27	theme	bleeding	179:186	arg1	wounds					188:193	irregularly shaped bleeding wounds	160:193	irregularly shaped bleeding wounds	160:193	Development of an injectable hemostatic for treating noncompressible or irregularly shaped bleeding wounds remains a pressing medical need.					
36302486	2	28	theme	chitosan/glycerophosphate	274:298	arg1	formulation					300:310	an injectable thermogelling chitosan/glycerophosphate formulation	246:310	an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA)	246:417	Herein, we report an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA).					
36302486	3	29	theme	hydrogel	503:510	arg1	structure					529:537	hydrogel internal network structure	503:537	hydrogel internal network structure	503:537	This was found to decrease gelation time by >2 times around 37 °C while increasing hydrogel internal network structure, with its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported.					
36302486	6	30	theme	supramolecular	1186:1199	arg1	mechanisms					1201:1210	noncovalent supramolecular mechanisms	1174:1210	noncovalent supramolecular mechanisms	1174:1210	Moreover, our results displayed the potential of a facile approach to enhance thermogelling and bioadhesion of chitosan-based hydrogels via noncovalent supramolecular mechanisms.					
36302486	2	31	theme	thermogelling	260:272	arg1	formulation					300:310	an injectable thermogelling chitosan/glycerophosphate formulation	246:310	an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA)	246:417	Herein, we report an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA).					
36302486	5	32	theme	blood	931:935	arg1	components					937:946	blood components	931:946	blood components	931:946	These improvements are biocompatible, without adversely affecting cell viability, blood components, biodegradability, or introducing notable inflammation, thus enabling injury healing.					
36302486	3	33	theme	internal	512:519	arg1	structure					529:537	hydrogel internal network structure	503:537	hydrogel internal network structure	503:537	This was found to decrease gelation time by >2 times around 37 °C while increasing hydrogel internal network structure, with its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported.					
36302486	2	34	theme	injectable	249:258	arg1	formulation					300:310	an injectable thermogelling chitosan/glycerophosphate formulation	246:310	an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA)	246:417	Herein, we report an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA).					
36302486	0	35	theme	efficient	66:74	arg1	hemostasis					76:85	efficient hemostasis	66:85	efficient hemostasis	66:85	Injectable thermogelling bioadhesive chitosan-based hydrogels for efficient hemostasis.					
36302486	3	36	theme	reported	645:652	arg1	strength					565:572	its tissue adhesive strength	545:572	its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported	545:652	This was found to decrease gelation time by >2 times around 37 °C while increasing hydrogel internal network structure, with its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported.					
36302486	3	37	theme	tissue	549:554	arg1	strength					565:572	its tissue adhesive strength	545:572	its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported	545:652	This was found to decrease gelation time by >2 times around 37 °C while increasing hydrogel internal network structure, with its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported.					
36302486	2	38	theme	gel-forming	326:336	arg1	capacity					338:345	gel-forming capacity	326:345	gel-forming capacity	326:345	Herein, we report an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA).					
36302486	2	39	theme	acid	407:410	arg1	incorporation					375:387	incorporation	375:387	incorporation of dihydrocaffeic acid (DHCA)	375:417	Herein, we report an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA).					
36302486	4	40	theme	amputation	830:839	arg1	models					841:846	rat hepatic hemorrhage and tail amputation models	798:846	rat hepatic hemorrhage and tail amputation models	798:846	The thermosensitive hydrogels significantly reduce whole blood coagulation time in vitro and both hemostasis time and blood loss in vivo using rat hepatic hemorrhage and tail amputation models.					
36302486	2	41	theme	dihydrocaffeic	392:405	arg1	DHCA					413:416	DHCA	413:416	DHCA	413:416	Herein, we report an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA).					
36302486	2	41	theme	dihydrocaffeic	392:405	arg1	acid					407:410	dihydrocaffeic acid	392:410	dihydrocaffeic acid (DHCA)	392:417	Herein, we report an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA).					
36302486	4	42	theme	thermosensitive	659:673	arg1	hydrogels					675:683	The thermosensitive hydrogels	655:683	The thermosensitive hydrogels	655:683	The thermosensitive hydrogels significantly reduce whole blood coagulation time in vitro and both hemostasis time and blood loss in vivo using rat hepatic hemorrhage and tail amputation models.					
36302486	4	43	theme	coagulation	718:728	arg1	time					730:733	whole blood coagulation time	706:733	whole blood coagulation time in vitro	706:742	The thermosensitive hydrogels significantly reduce whole blood coagulation time in vitro and both hemostasis time and blood loss in vivo using rat hepatic hemorrhage and tail amputation models.					
36302486	4	44	theme	blood	712:716	arg1	time					730:733	whole blood coagulation time	706:733	whole blood coagulation time in vitro	706:742	The thermosensitive hydrogels significantly reduce whole blood coagulation time in vitro and both hemostasis time and blood loss in vivo using rat hepatic hemorrhage and tail amputation models.					
36302486	3	45	theme	>2	574:575	arg1	times					577:581	>2 times	574:581	>2 times	574:581	This was found to decrease gelation time by >2 times around 37 °C while increasing hydrogel internal network structure, with its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported.					
36302486	6	46	theme	noncovalent	1174:1184	arg1	mechanisms					1201:1210	noncovalent supramolecular mechanisms	1174:1210	noncovalent supramolecular mechanisms	1174:1210	Moreover, our results displayed the potential of a facile approach to enhance thermogelling and bioadhesion of chitosan-based hydrogels via noncovalent supramolecular mechanisms.					
36302486	4	47	theme	hemostasis	753:762	arg1	time					764:767	hemostasis time	753:767	hemostasis time	753:767	The thermosensitive hydrogels significantly reduce whole blood coagulation time in vitro and both hemostasis time and blood loss in vivo using rat hepatic hemorrhage and tail amputation models.					
36302486	6	48	theme	approach	1092:1099	arg1	potential					1070:1078	the potential	1066:1078	the potential of a facile approach to enhance thermogelling and bioadhesion of chitosan-based hydrogels via noncovalent supramolecular mechanisms	1066:1210	Moreover, our results displayed the potential of a facile approach to enhance thermogelling and bioadhesion of chitosan-based hydrogels via noncovalent supramolecular mechanisms.					
37285678	0	0	theme	pig	78:80	arg1	house					82:86	a pig house	76:86	a pig house	76:86	Pectin alleviates the pulmonary inflammatory response induced by PM2.5 from a pig house by modulating intestinal microbiota.					
37285678	7	1	theme	Bacteroidetes	1228:1240	arg1	abundance					1215:1223	the relative abundance	1202:1223	the relative abundance of Bacteroidetes	1202:1240	Dietary pectin altered the composition of the intestinal microbiota, increasing the relative abundance of Bacteroidetes and decreasing the ratio of Firmicutes/Bacteroidetes.					
37285678	6	2	theme	lavage	1024:1029	arg1	BLAF					1038:1041	BLAF	1038:1041	BLAF	1038:1041	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	6	2	theme	lavage	1024:1029	arg1	fluid					1031:1035	bronchoalveolar lavage fluid	1008:1035	bronchoalveolar lavage fluid (BLAF)	1008:1042	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	6	3	theme	IL-1β	948:952	arg1	levels					938:943	mRNA expression levels	922:943	mRNA expression levels of IL-1β, IL-6 and IL-17	922:968	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	10	4	theme	intestinal	1754:1763	arg1	composition					1776:1786	intestinal microbiota composition	1754:1786	intestinal microbiota composition	1754:1786	In conclusion, dietary fermentable fiber pectin can relieve PM2.5-induced pulmonary inflammation via alteration of intestinal microbiota composition and SCFA production.					
37285678	8	5	theme	acid	1334:1337	arg1	Ruminococcus					1433:1444	Ruminococcus 2	1433:1446	Ruminococcus 2	1433:1446	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	8	5	theme	acid	1334:1337	arg1	bacteria					1356:1363	short-chain fatty acid (SCFA)-producing bacteria	1316:1363	short-chain fatty acid (SCFA)-producing bacteria	1316:1363	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	8	5	theme	acid	1334:1337	arg1	Prevotella					1402:1411	Prevotella	1402:1411	Prevotella	1402:1411	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	8	5	theme	acid	1334:1337	arg1	Butyricimonas					1452:1464	Butyricimonas	1452:1464	Butyricimonas	1452:1464	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	8	5	theme	acid	1334:1337	arg1	Parabacteroides					1416:1430	Parabacteroides	1416:1430	Parabacteroides	1416:1430	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	8	5	theme	acid	1334:1337	arg1	Anaerotruncus					1387:1399	Anaerotruncus	1387:1399	Anaerotruncus	1387:1399	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	11	6	theme	health	1861:1866	arg1	risk					1868:1871	the health risk	1857:1871	the health risk associated with PM2.5 exposure	1857:1902	This study provides a new insight into reducing the health risk associated with PM2.5 exposure.					
37285678	2	7	theme	nursery	302:308	arg1	house					314:318	a nursery pig house	300:318	a nursery pig house	300:318	PM2.5 samples were collected from a nursery pig house.					
37285678	10	8	theme	dietary	1654:1660	arg1	fiber					1674:1678	dietary fermentable fiber	1654:1678	dietary fermentable fiber pectin	1654:1685	In conclusion, dietary fermentable fiber pectin can relieve PM2.5-induced pulmonary inflammation via alteration of intestinal microbiota composition and SCFA production.					
37285678	4	9	theme	+ pectin	564:571	arg1	group					573:577	the PM2.5 + pectin group	554:577	the PM2.5 + pectin group	554:577	The mice in the PM2.5 group were intratracheally instilled with PM2.5 suspension twice a week for four consecutive weeks, and those in the PM2.5 + pectin group were subject to the same PM2.5 exposure, but fed with a basal diet supplemented with 5% pectin.					
37285678	6	10	theme	pulmonary	839:847	arg1	inflammation					849:860	PM2.5-induced pulmonary inflammation	825:860	PM2.5-induced pulmonary inflammation	825:860	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	9	11	theme	SCFAs	1569:1573	arg1	butyrate					1607:1614	butyrate	1607:1614	butyrate	1607:1614	Accordingly, dietary pectin increased the concentrations of SCFAs, including acetate, propionate, butyrate and valerate, in mice.					
37285678	9	11	theme	SCFAs	1569:1573	arg1	propionate					1595:1604	propionate	1595:1604	propionate	1595:1604	Accordingly, dietary pectin increased the concentrations of SCFAs, including acetate, propionate, butyrate and valerate, in mice.					
37285678	9	11	theme	SCFAs	1569:1573	arg1	acetate					1586:1592	acetate	1586:1592	acetate	1586:1592	Accordingly, dietary pectin increased the concentrations of SCFAs, including acetate, propionate, butyrate and valerate, in mice.					
37285678	9	11	theme	SCFAs	1569:1573	arg1	concentrations					1551:1564	the concentrations	1547:1564	the concentrations	1547:1564	Accordingly, dietary pectin increased the concentrations of SCFAs, including acetate, propionate, butyrate and valerate, in mice.					
37285678	9	11	theme	SCFAs	1569:1573	arg1	valerate					1620:1627	valerate	1620:1627	valerate	1620:1627	Accordingly, dietary pectin increased the concentrations of SCFAs, including acetate, propionate, butyrate and valerate, in mice.					
37285678	6	12	theme	restored	886:893	arg1	morphology					900:909	slightly restored lung morphology	877:909	slightly restored lung morphology	877:909	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	6	13	theme	IL-6	1092:1095	arg1	levels					1072:1077	protein levels	1064:1077	protein levels of IL-1β and IL-6 in the serum (p < 0.05)	1064:1119	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	7	14	theme	intestinal	1168:1177	arg1	microbiota					1179:1188	the intestinal microbiota	1164:1188	the intestinal microbiota	1164:1188	Dietary pectin altered the composition of the intestinal microbiota, increasing the relative abundance of Bacteroidetes and decreasing the ratio of Firmicutes/Bacteroidetes.					
37285678	0	15	theme	intestinal	102:111	arg1	microbiota					113:122	intestinal microbiota	102:122	intestinal microbiota	102:122	Pectin alleviates the pulmonary inflammatory response induced by PM2.5 from a pig house by modulating intestinal microbiota.					
37285678	6	16	theme	PM2.5-induced	825:837	arg1	inflammation					849:860	PM2.5-induced pulmonary inflammation	825:860	PM2.5-induced pulmonary inflammation	825:860	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	6	17	theme	bronchoalveolar	1008:1022	arg1	BLAF					1038:1041	BLAF	1038:1041	BLAF	1038:1041	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	6	17	theme	bronchoalveolar	1008:1022	arg1	fluid					1031:1035	bronchoalveolar lavage fluid	1008:1035	bronchoalveolar lavage fluid (BLAF)	1008:1042	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	1	18	theme	dietary	165:171	arg1	pectin					179:184	dietary fiber pectin	165:184	dietary fiber pectin	165:184	This study aimed to investigate whether dietary fiber pectin can alleviate PM2.5-induced pulmonary inflammation and the potential mechanism.					
37285678	6	19	theme	IL-1β	1082:1086	arg1	levels					1072:1077	protein levels	1064:1077	protein levels of IL-1β and IL-6 in the serum (p < 0.05)	1064:1119	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	1	20	theme	fiber	173:177	arg1	pectin					179:184	dietary fiber pectin	165:184	dietary fiber pectin	165:184	This study aimed to investigate whether dietary fiber pectin can alleviate PM2.5-induced pulmonary inflammation and the potential mechanism.					
37285678	7	21	dep	altered	1137:1143	arg1	increasing					1191:1200	increasing	1191:1200	increasing the relative abundance of Bacteroidetes	1191:1240	Dietary pectin altered the composition of the intestinal microbiota, increasing the relative abundance of Bacteroidetes and decreasing the ratio of Firmicutes/Bacteroidetes.					
37285678	7	21	dep	altered	1137:1143	arg1	decreasing					1246:1255	decreasing	1246:1255	decreasing the ratio of Firmicutes/Bacteroidetes	1246:1293	Dietary pectin altered the composition of the intestinal microbiota, increasing the relative abundance of Bacteroidetes and decreasing the ratio of Firmicutes/Bacteroidetes.					
37285678	3	22	theme	PM2.5	381:385	arg1	groups					354:359	three groups	348:359	three groups: the control group, PM2.5 group and PM2.5 + pectin group	348:416	The mice were divided into three groups: the control group, PM2.5 group and PM2.5 + pectin group.					
37285678	3	22	theme	PM2.5	381:385	arg1	group					387:391	PM2.5 group	381:391	PM2.5 group	381:391	The mice were divided into three groups: the control group, PM2.5 group and PM2.5 + pectin group.					
37285678	6	23	theme	expression	927:936	arg1	levels					938:943	mRNA expression levels	922:943	mRNA expression levels of IL-1β, IL-6 and IL-17	922:968	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	9	24	theme	dietary	1522:1528	arg1	pectin					1530:1535	dietary pectin	1522:1535	dietary pectin	1522:1535	Accordingly, dietary pectin increased the concentrations of SCFAs, including acetate, propionate, butyrate and valerate, in mice.					
37285678	6	25	theme	MPO	993:995	arg1	content					997:1003	MPO content	993:1003	MPO content	993:1003	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	3	26	theme	control	366:372	arg1	groups					354:359	three groups	348:359	three groups: the control group, PM2.5 group and PM2.5 + pectin group	348:416	The mice were divided into three groups: the control group, PM2.5 group and PM2.5 + pectin group.					
37285678	3	26	theme	control	366:372	arg1	group					374:378	the control group	362:378	the control group	362:378	The mice were divided into three groups: the control group, PM2.5 group and PM2.5 + pectin group.					
37285678	6	27	theme	mRNA	922:925	arg1	levels					938:943	mRNA expression levels	922:943	mRNA expression levels of IL-1β, IL-6 and IL-17	922:968	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	6	28	theme	protein	1064:1070	arg1	levels					1072:1077	protein levels	1064:1077	protein levels of IL-1β and IL-6 in the serum (p < 0.05)	1064:1119	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	4	29	theme	same	599:602	arg1	exposure					610:617	the same PM2.5 exposure	595:617	the same PM2.5 exposure	595:617	The mice in the PM2.5 group were intratracheally instilled with PM2.5 suspension twice a week for four consecutive weeks, and those in the PM2.5 + pectin group were subject to the same PM2.5 exposure, but fed with a basal diet supplemented with 5% pectin.					
37285678	10	30	theme	composition	1776:1786	arg1	alteration					1740:1749	alteration	1740:1749	alteration of intestinal microbiota composition and SCFA production	1740:1806	In conclusion, dietary fermentable fiber pectin can relieve PM2.5-induced pulmonary inflammation via alteration of intestinal microbiota composition and SCFA production.					
37285678	2	31	theme	pig	310:312	arg1	house					314:318	a nursery pig house	300:318	a nursery pig house	300:318	PM2.5 samples were collected from a nursery pig house.					
37285678	1	32	theme	PM2.5-induced	200:212	arg1	inflammation					224:235	PM2.5-induced pulmonary inflammation	200:235	PM2.5-induced pulmonary inflammation	200:235	This study aimed to investigate whether dietary fiber pectin can alleviate PM2.5-induced pulmonary inflammation and the potential mechanism.					
37285678	5	33	theme	feed	715:718	arg1	intake					720:725	feed intake	715:725	feed intake	715:725	The results showed that body weight and feed intake were not different among the treatments (p > 0.05).					
37285678	10	34	theme	fermentable	1662:1672	arg1	fiber					1674:1678	dietary fermentable fiber	1654:1678	dietary fermentable fiber pectin	1654:1685	In conclusion, dietary fermentable fiber pectin can relieve PM2.5-induced pulmonary inflammation via alteration of intestinal microbiota composition and SCFA production.					
37285678	8	35	theme	-producing	1345:1354	arg1	Ruminococcus					1433:1444	Ruminococcus 2	1433:1446	Ruminococcus 2	1433:1446	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	8	35	theme	-producing	1345:1354	arg1	bacteria					1356:1363	short-chain fatty acid (SCFA)-producing bacteria	1316:1363	short-chain fatty acid (SCFA)-producing bacteria	1316:1363	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	8	35	theme	-producing	1345:1354	arg1	Prevotella					1402:1411	Prevotella	1402:1411	Prevotella	1402:1411	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	8	35	theme	-producing	1345:1354	arg1	Butyricimonas					1452:1464	Butyricimonas	1452:1464	Butyricimonas	1452:1464	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	8	35	theme	-producing	1345:1354	arg1	Parabacteroides					1416:1430	Parabacteroides	1416:1430	Parabacteroides	1416:1430	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	8	35	theme	-producing	1345:1354	arg1	Anaerotruncus					1387:1399	Anaerotruncus	1387:1399	Anaerotruncus	1387:1399	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	10	36	theme	pulmonary	1713:1721	arg1	inflammation					1723:1734	PM2.5-induced pulmonary inflammation	1699:1734	PM2.5-induced pulmonary inflammation	1699:1734	In conclusion, dietary fermentable fiber pectin can relieve PM2.5-induced pulmonary inflammation via alteration of intestinal microbiota composition and SCFA production.					
37285678	6	37	from	levels	1072:1077	arg1	p < 0.05					1111:1118	p < 0.05	1111:1118	p < 0.05	1111:1118	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	6	37	from	levels	1072:1077	arg1	serum					1104:1108	the serum	1100:1108	the serum (p < 0.05)	1100:1119	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	1	38	theme	pulmonary	214:222	arg1	inflammation					224:235	PM2.5-induced pulmonary inflammation	200:235	PM2.5-induced pulmonary inflammation	200:235	This study aimed to investigate whether dietary fiber pectin can alleviate PM2.5-induced pulmonary inflammation and the potential mechanism.					
37285678	0	39	theme	inflammatory	32:43	arg1	response					45:52	the pulmonary inflammatory response	18:52	the pulmonary inflammatory response induced by PM2.5 from a pig house by modulating intestinal microbiota	18:122	Pectin alleviates the pulmonary inflammatory response induced by PM2.5 from a pig house by modulating intestinal microbiota.					
37285678	3	40	theme	+ pectin	403:410	arg1	groups					354:359	three groups	348:359	three groups: the control group, PM2.5 group and PM2.5 + pectin group	348:416	The mice were divided into three groups: the control group, PM2.5 group and PM2.5 + pectin group.					
37285678	3	40	theme	+ pectin	403:410	arg1	group					412:416	PM2.5 + pectin group	397:416	PM2.5 + pectin group	397:416	The mice were divided into three groups: the control group, PM2.5 group and PM2.5 + pectin group.					
37285678	10	41	theme	microbiota	1765:1774	arg1	composition					1776:1786	intestinal microbiota composition	1754:1786	intestinal microbiota composition	1754:1786	In conclusion, dietary fermentable fiber pectin can relieve PM2.5-induced pulmonary inflammation via alteration of intestinal microbiota composition and SCFA production.					
37285678	0	42	theme	pulmonary	22:30	arg1	response					45:52	the pulmonary inflammatory response	18:52	the pulmonary inflammatory response induced by PM2.5 from a pig house by modulating intestinal microbiota	18:122	Pectin alleviates the pulmonary inflammatory response induced by PM2.5 from a pig house by modulating intestinal microbiota.					
37285678	10	43	dep	fiber	1674:1678	arg1	pectin					1680:1685	pectin	1680:1685	dietary fermentable fiber pectin	1654:1685	In conclusion, dietary fermentable fiber pectin can relieve PM2.5-induced pulmonary inflammation via alteration of intestinal microbiota composition and SCFA production.					
37285678	4	44	theme	PM2.5	604:608	arg1	exposure					610:617	the same PM2.5 exposure	595:617	the same PM2.5 exposure	595:617	The mice in the PM2.5 group were intratracheally instilled with PM2.5 suspension twice a week for four consecutive weeks, and those in the PM2.5 + pectin group were subject to the same PM2.5 exposure, but fed with a basal diet supplemented with 5% pectin.					
37285678	6	45	theme	lung	895:898	arg1	morphology					900:909	slightly restored lung morphology	877:909	slightly restored lung morphology	877:909	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	4	46	from	mice	423:426	arg1	group					441:445	the PM2.5 group	431:445	the PM2.5 group	431:445	The mice in the PM2.5 group were intratracheally instilled with PM2.5 suspension twice a week for four consecutive weeks, and those in the PM2.5 + pectin group were subject to the same PM2.5 exposure, but fed with a basal diet supplemented with 5% pectin.					
37285678	4	47	theme	PM2.5	435:439	arg1	group					441:445	the PM2.5 group	431:445	the PM2.5 group	431:445	The mice in the PM2.5 group were intratracheally instilled with PM2.5 suspension twice a week for four consecutive weeks, and those in the PM2.5 + pectin group were subject to the same PM2.5 exposure, but fed with a basal diet supplemented with 5% pectin.					
37285678	8	48	theme	fatty	1328:1332	arg1	SCFA					1340:1343	SCFA	1340:1343	SCFA	1340:1343	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	8	48	theme	fatty	1328:1332	arg1	acid					1334:1337	short-chain fatty acid	1316:1337	short-chain fatty acid (SCFA)-producing bacteria	1316:1363	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	4	49	theme	5	664:664	arg1	%					665:665	%	665:665	%	665:665	The mice in the PM2.5 group were intratracheally instilled with PM2.5 suspension twice a week for four consecutive weeks, and those in the PM2.5 + pectin group were subject to the same PM2.5 exposure, but fed with a basal diet supplemented with 5% pectin.					
37285678	4	50	theme	consecutive	522:532	arg1	weeks					534:538	four consecutive weeks	517:538	four consecutive weeks	517:538	The mice in the PM2.5 group were intratracheally instilled with PM2.5 suspension twice a week for four consecutive weeks, and those in the PM2.5 + pectin group were subject to the same PM2.5 exposure, but fed with a basal diet supplemented with 5% pectin.					
37285678	3	51	dep	groups	354:359	arg1	groups					354:359	three groups	348:359	three groups: the control group, PM2.5 group and PM2.5 + pectin group	348:416	The mice were divided into three groups: the control group, PM2.5 group and PM2.5 + pectin group.					
37285678	3	51	dep	groups	354:359	arg1	group					412:416	PM2.5 + pectin group	397:416	PM2.5 + pectin group	397:416	The mice were divided into three groups: the control group, PM2.5 group and PM2.5 + pectin group.					
37285678	3	51	dep	groups	354:359	arg1	group					374:378	the control group	362:378	the control group	362:378	The mice were divided into three groups: the control group, PM2.5 group and PM2.5 + pectin group.					
37285678	3	51	dep	groups	354:359	arg1	group					387:391	PM2.5 group	381:391	PM2.5 group	381:391	The mice were divided into three groups: the control group, PM2.5 group and PM2.5 + pectin group.					
37285678	6	52	with	supplementation	788:802	arg1	pectin					809:814	pectin	809:814	pectin	809:814	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	8	53	theme	short-chain	1316:1326	arg1	SCFA					1340:1343	SCFA	1340:1343	SCFA	1340:1343	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	8	53	theme	short-chain	1316:1326	arg1	acid					1334:1337	short-chain fatty acid	1316:1337	short-chain fatty acid (SCFA)-producing bacteria	1316:1363	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	7	54	theme	microbiota	1179:1188	arg1	composition					1149:1159	the composition	1145:1159	the composition of the intestinal microbiota	1145:1188	Dietary pectin altered the composition of the intestinal microbiota, increasing the relative abundance of Bacteroidetes and decreasing the ratio of Firmicutes/Bacteroidetes.					
37285678	10	55	theme	PM2.5-induced	1699:1711	arg1	inflammation					1723:1734	PM2.5-induced pulmonary inflammation	1699:1734	PM2.5-induced pulmonary inflammation	1699:1734	In conclusion, dietary fermentable fiber pectin can relieve PM2.5-induced pulmonary inflammation via alteration of intestinal microbiota composition and SCFA production.					
37285678	8	56	theme	genus	1303:1307	arg1	level					1309:1313	the genus level	1299:1313	the genus level	1299:1313	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	10	57	theme	production	1797:1806	arg1	alteration					1740:1749	alteration	1740:1749	alteration of intestinal microbiota composition and SCFA production	1740:1806	In conclusion, dietary fermentable fiber pectin can relieve PM2.5-induced pulmonary inflammation via alteration of intestinal microbiota composition and SCFA production.					
37285678	7	58	theme	Firmicutes/Bacteroidetes	1270:1293	arg1	ratio					1261:1265	the ratio	1257:1265	the ratio of Firmicutes/Bacteroidetes	1257:1293	Dietary pectin altered the composition of the intestinal microbiota, increasing the relative abundance of Bacteroidetes and decreasing the ratio of Firmicutes/Bacteroidetes.					
37285678	4	59	theme	%	665:665	arg1	pectin					667:672	5% pectin	664:672	5% pectin	664:672	The mice in the PM2.5 group were intratracheally instilled with PM2.5 suspension twice a week for four consecutive weeks, and those in the PM2.5 + pectin group were subject to the same PM2.5 exposure, but fed with a basal diet supplemented with 5% pectin.					
37285678	6	60	theme	IL-17	964:968	arg1	levels					938:943	mRNA expression levels	922:943	mRNA expression levels of IL-1β, IL-6 and IL-17	922:968	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37285678	7	61	theme	Dietary	1122:1128	arg1	pectin					1130:1135	Dietary pectin	1122:1135	Dietary pectin	1122:1135	Dietary pectin altered the composition of the intestinal microbiota, increasing the relative abundance of Bacteroidetes and decreasing the ratio of Firmicutes/Bacteroidetes.					
37285678	10	62	theme	SCFA	1792:1795	arg1	production					1797:1806	SCFA production	1792:1806	SCFA production	1792:1806	In conclusion, dietary fermentable fiber pectin can relieve PM2.5-induced pulmonary inflammation via alteration of intestinal microbiota composition and SCFA production.					
37285678	2	63	theme	PM2.5	266:270	arg1	samples					272:278	PM2.5 samples	266:278	PM2.5 samples	266:278	PM2.5 samples were collected from a nursery pig house.					
37285678	7	64	theme	relative	1206:1213	arg1	abundance					1215:1223	the relative abundance	1202:1223	the relative abundance of Bacteroidetes	1202:1240	Dietary pectin altered the composition of the intestinal microbiota, increasing the relative abundance of Bacteroidetes and decreasing the ratio of Firmicutes/Bacteroidetes.					
37285678	4	65	theme	basal	635:639	arg1	diet					641:644	a basal diet	633:644	a basal diet supplemented with 5% pectin	633:672	The mice in the PM2.5 group were intratracheally instilled with PM2.5 suspension twice a week for four consecutive weeks, and those in the PM2.5 + pectin group were subject to the same PM2.5 exposure, but fed with a basal diet supplemented with 5% pectin.					
37285678	11	66	theme	new	1831:1833	arg1	insight					1835:1841	a new insight	1829:1841	a new insight into reducing the health risk associated with PM2.5 exposure	1829:1902	This study provides a new insight into reducing the health risk associated with PM2.5 exposure.					
37285678	1	67	theme	potential	245:253	arg1	mechanism					255:263	the potential mechanism	241:263	the potential mechanism	241:263	This study aimed to investigate whether dietary fiber pectin can alleviate PM2.5-induced pulmonary inflammation and the potential mechanism.					
37285678	5	68	theme	body	699:702	arg1	weight					704:709	body weight	699:709	body weight	699:709	The results showed that body weight and feed intake were not different among the treatments (p > 0.05).					
37285678	8	69	theme	+pectin	1494:1500	arg1	group					1502:1506	the PM2.5 +pectin group	1484:1506	the PM2.5 +pectin group	1484:1506	At the genus level, short-chain fatty acid (SCFA)-producing bacteria, such as Bacteroides, Anaerotruncus, Prevotella 2, Parabacteroides, Ruminococcus 2 and Butyricimonas, were enriched in the PM2.5 +pectin group.					
37285678	6	70	theme	IL-6	955:958	arg1	levels					938:943	mRNA expression levels	922:943	mRNA expression levels of IL-1β, IL-6 and IL-17	922:968	However, supplementation with pectin relieved PM2.5-induced pulmonary inflammation, presenting as slightly restored lung morphology, decreased mRNA expression levels of IL-1β, IL-6 and IL-17 in the lung, decreased MPO content in bronchoalveolar lavage fluid (BLAF), and even decreased protein levels of IL-1β and IL-6 in the serum (p < 0.05).					
37245771	8	0	theme	gas	1287:1289	arg1	spectroscopy					1311:1322	gas chromatography-mass spectroscopy	1287:1322	gas chromatography-mass spectroscopy (GC-MS)	1287:1330	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	8	0	theme	gas	1287:1289	arg1	GC-MS					1325:1329	GC-MS	1325:1329	GC-MS	1325:1329	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	12	1	theme	loaded	1892:1897	arg1	oil					1909:1911	the loaded essential oil	1888:1911	the loaded essential oil	1888:1911	This transition leads to a facile release of the loaded essential oil.					
37245771	13	2	theme	release	1918:1924	arg1	test					1926:1929	The release test	1914:1929	The release test	1914:1929	The release test depicts that about 30 % of Zataria multiflora essential oil is released during the first 16 min.					
37245771	2	3	theme	promising	412:420	arg1	they					403:406	they	403:406	they	403:406	Biomaterial-based hydrogels have been extensively used in biomedical applications, and they are promising platforms to encapsulate essential oils.					
37245771	2	3	theme	promising	412:420	arg1	platforms					422:430	promising platforms	412:430	promising platforms	412:430	Biomaterial-based hydrogels have been extensively used in biomedical applications, and they are promising platforms to encapsulate essential oils.					
37245771	4	4	theme	polyvinyl	685:693	arg1	hydrogel					720:727	a polyvinyl alcohol/chitosan/gelatin hydrogel	683:727	a polyvinyl alcohol/chitosan/gelatin hydrogel	683:727	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	10	5	theme	essential	1659:1667	arg1	constituents					1673:1684	the essential oil constituents	1655:1684	the essential oil constituents	1655:1684	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37245771	12	6	theme	facile	1870:1875	arg1	release					1877:1883	a facile release	1868:1883	a facile release of the loaded essential oil	1868:1911	This transition leads to a facile release of the loaded essential oil.					
37245771	11	7	theme	thermo-responsive	1747:1763	arg1	hydrogel					1765:1772	the produced thermo-responsive hydrogel	1734:1772	the produced thermo-responsive hydrogel	1734:1772	Based on the rheological results, the produced thermo-responsive hydrogel shows a gel-sol viscoelastic transition at a temperature of 24.5 °C.					
37245771	15	8	theme	potential	2248:2256	arg1	platform					2284:2291	a potential intelligent drug delivery platform	2246:2291	a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems	2246:2454	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	15	8	theme	potential	2248:2256	arg1	hydrogel					2220:2227	The fabricated hydrogel	2205:2227	The fabricated hydrogel	2205:2227	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	10	9	theme	chitosan	1690:1697	arg1	activity					1643:1650	the antifungal activity	1628:1650	the antifungal activity of the essential oil constituents and chitosan	1628:1697	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37245771	10	10	theme	produced	1513:1520	arg1	hydrogel					1522:1529	The produced hydrogel	1509:1529	The produced hydrogel	1509:1529	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37245771	2	11	theme	biomedical	374:383	arg1	applications					385:396	biomedical applications	374:396	biomedical applications	374:396	Biomaterial-based hydrogels have been extensively used in biomedical applications, and they are promising platforms to encapsulate essential oils.					
37245771	1	12	theme	natural	225:231	arg1	oil					216:218	Zataria multiflora essential oil	187:218	Zataria multiflora essential oil	187:218	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	1	12	theme	natural	225:231	arg1	product					248:254	a natural volatile plant product	223:254	a natural volatile plant product whose therapeutic applications require a delivery platform	223:313	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	4	13	theme	thermo-responsive	743:759	arg1	oil					660:662	Zataria multiflora essential oil	631:662	Zataria multiflora essential oil	631:662	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	4	13	theme	thermo-responsive	743:759	arg1	platform					776:783	a positive thermo-responsive and antifungal platform	732:783	a positive thermo-responsive and antifungal platform	732:783	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	10	14	theme	constituents	1673:1684	arg1	activity					1643:1650	the antifungal activity	1628:1650	the antifungal activity of the essential oil constituents and chitosan	1628:1697	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37245771	5	15	theme	imaging	955:961	arg1	results					963:969	the SEM imaging results	947:969	the SEM imaging results	947:969	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	4	16	theme	antifungal	765:774	arg1	oil					660:662	Zataria multiflora essential oil	631:662	Zataria multiflora essential oil	631:662	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	4	16	theme	antifungal	765:774	arg1	platform					776:783	a positive thermo-responsive and antifungal platform	732:783	a positive thermo-responsive and antifungal platform	732:783	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	0	17	theme	biological	160:169	arg1	investigations					171:184	biological investigations	160:184	biological investigations	160:184	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	7	18	theme	oil	1152:1154	arg1	encapsulation					1102:1114	the successful efficient encapsulation	1077:1114	the successful efficient encapsulation of the Zataria multiflora essential oil within the hydrogel	1077:1174	These results confirm the successful efficient encapsulation of the Zataria multiflora essential oil within the hydrogel.					
37245771	8	19	dep	Zataria	1210:1216	arg1	multiflora					1218:1227	multiflora	1218:1227	multiflora	1218:1227	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	5	20	theme	optical	803:809	arg1	image					823:827	the optical microscopic image	799:827	the optical microscopic image	799:827	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	7	21	theme	multiflora	1131:1140	arg1	oil					1152:1154	the Zataria multiflora essential oil	1119:1154	the Zataria multiflora essential oil	1119:1154	These results confirm the successful efficient encapsulation of the Zataria multiflora essential oil within the hydrogel.					
37245771	15	22	theme	drug	2434:2437	arg1	systems					2448:2454	traditional drug delivery systems	2422:2454	traditional drug delivery systems	2422:2454	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	1	23	theme	volatile	233:240	arg1	oil					216:218	Zataria multiflora essential oil	187:218	Zataria multiflora essential oil	187:218	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	1	23	theme	volatile	233:240	arg1	product					248:254	a natural volatile plant product	223:254	a natural volatile plant product whose therapeutic applications require a delivery platform	223:313	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	13	24	theme	first	2014:2018	arg1	16 min					2020:2025	the first 16 min	2010:2025	the first 16 min	2010:2025	The release test depicts that about 30 % of Zataria multiflora essential oil is released during the first 16 min.					
37245771	5	25	theme	mean	889:892	arg1	consistent					931:940	consistent	931:940	consistent	931:940	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	5	25	theme	mean	889:892	arg1	size					894:897	a mean size	887:897	a mean size	887:897	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	1	26	theme	Zataria	187:193	arg1	oil					216:218	Zataria multiflora essential oil	187:218	Zataria multiflora essential oil	187:218	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	1	26	theme	Zataria	187:193	arg1	product					248:254	a natural volatile plant product	223:254	a natural volatile plant product whose therapeutic applications require a delivery platform	223:313	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	13	27	theme	essential	1977:1985	arg1	oil					1987:1989	Zataria multiflora essential oil	1958:1989	Zataria multiflora essential oil	1958:1989	The release test depicts that about 30 % of Zataria multiflora essential oil is released during the first 16 min.					
37245771	0	28	theme	hydrogel	106:113	arg1	Synthesis					116:124	polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis	53:124	polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis	53:124	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	7	29	theme	successful	1081:1090	arg1	encapsulation					1102:1114	the successful efficient encapsulation	1077:1114	the successful efficient encapsulation of the Zataria multiflora essential oil within the hydrogel	1077:1174	These results confirm the successful efficient encapsulation of the Zataria multiflora essential oil within the hydrogel.					
37245771	1	30	theme	essential	206:214	arg1	oil					216:218	Zataria multiflora essential oil	187:218	Zataria multiflora essential oil	187:218	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	1	30	theme	essential	206:214	arg1	product					248:254	a natural volatile plant product	223:254	a natural volatile plant product whose therapeutic applications require a delivery platform	223:313	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	0	31	from	Encapsulation	0:12	arg1	investigations					171:184	biological investigations	160:184	biological investigations	160:184	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	0	31	from	Encapsulation	0:12	arg1	Synthesis					116:124	polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis	53:124	polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis	53:124	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	0	31	from	Encapsulation	0:12	arg1	properties					144:153	physico-chemical properties	127:153	physico-chemical properties	127:153	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	10	32	theme	metabolic	1544:1552	arg1	activity					1554:1561	the metabolic activity	1540:1561	the metabolic activity	1540:1561	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37245771	10	32	theme	metabolic	1544:1552	arg1	related					1617:1623	related	1617:1623	related	1617:1623	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37245771	5	33	theme	oil	867:869	arg1	droplets					871:878	the encapsulated spherical essential oil droplets	830:878	the encapsulated spherical essential oil droplets	830:878	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	0	34	theme	physico-chemical	127:142	arg1	properties					144:153	physico-chemical properties	127:153	physico-chemical properties	127:153	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	0	35	theme	oil	46:48	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.	0:185	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	10	36	theme	albicans	1574:1581	arg1	activity					1554:1561	the metabolic activity	1540:1561	the metabolic activity	1540:1561	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37245771	10	36	theme	albicans	1574:1581	arg1	related					1617:1623	related	1617:1623	related	1617:1623	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37245771	13	37	theme	Zataria	1958:1964	arg1	oil					1987:1989	Zataria multiflora essential oil	1958:1989	Zataria multiflora essential oil	1958:1989	The release test depicts that about 30 % of Zataria multiflora essential oil is released during the first 16 min.					
37245771	14	38	theme	MTT	2079:2081	arg1	assay					2084:2088	2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay	2041:2088	assay	2084:2088	In addition, 2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay demonstrates that the designed thermo-sensitive formulation is biocompatible with high cell viability (over 96 %).					
37245771	4	39	theme	multiflora	639:648	arg1	oil					660:662	Zataria multiflora essential oil	631:662	Zataria multiflora essential oil	631:662	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	4	39	theme	multiflora	639:648	arg1	platform					776:783	a positive thermo-responsive and antifungal platform	732:783	a positive thermo-responsive and antifungal platform	732:783	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	14	40	theme	cell	2177:2180	arg1	viability					2182:2190	high cell viability	2172:2190	high cell viability (over 96 %)	2172:2202	In addition, 2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay demonstrates that the designed thermo-sensitive formulation is biocompatible with high cell viability (over 96 %).					
37245771	0	41	theme	Zataria	17:23	arg1	oil					46:48	Zataria multiflora essential oil	17:48	Zataria multiflora essential oil	17:48	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	8	42	theme	Zataria	1210:1216	arg1	oil					1239:1241	the Zataria multiflora essential oil	1206:1241	the Zataria multiflora essential oil	1206:1241	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	6	43	theme	loading	999:1005	arg1	efficacy					986:993	Encapsulation efficacy	972:993	Encapsulation efficacy	972:993	Encapsulation efficacy and loading capacity are 98.66 % and 12.98 %, respectively.					
37245771	6	43	theme	loading	999:1005	arg1	capacity					1007:1014	loading capacity	999:1014	loading capacity	999:1014	Encapsulation efficacy and loading capacity are 98.66 % and 12.98 %, respectively.					
37245771	6	43	theme	loading	999:1005	arg1	%					1026:1026	98.66 %	1020:1026	98.66 %	1020:1026	Encapsulation efficacy and loading capacity are 98.66 % and 12.98 %, respectively.					
37245771	14	44	theme	bromide	2070:2076	arg1	assay					2084:2088	2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay	2041:2088	assay	2084:2088	In addition, 2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay demonstrates that the designed thermo-sensitive formulation is biocompatible with high cell viability (over 96 %).					
37245771	0	45	theme	alcohol/chitosan/gelatin	63:86	arg1	Synthesis					116:124	polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis	53:124	polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis	53:124	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	3	46	theme	many	536:539	arg1	interests					541:549	many interests	536:549	many interests	536:549	Among different hydrogels, intelligent hydrogels have recently attracted many interests because of their response to environmental stimuli such as temperature.					
37245771	0	47	theme	essential	36:44	arg1	oil					46:48	Zataria multiflora essential oil	17:48	Zataria multiflora essential oil	17:48	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	15	48	theme	antifungal	2338:2347	arg1	alternative					2407:2417	a promising alternative	2395:2417	a promising alternative to traditional drug delivery systems	2395:2454	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	15	48	theme	antifungal	2338:2347	arg1	effectiveness					2349:2361	antifungal effectiveness	2338:2361	antifungal effectiveness	2338:2361	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	15	48	theme	antifungal	2338:2347	arg1	toxicity					2372:2379	less toxicity	2367:2379	less toxicity	2367:2379	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	8	49	theme	essential	1229:1237	arg1	oil					1239:1241	the Zataria multiflora essential oil	1206:1241	the Zataria multiflora essential oil	1206:1241	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	3	50	theme	different	469:477	arg1	hydrogels					479:487	different hydrogels	469:487	different hydrogels	469:487	Among different hydrogels, intelligent hydrogels have recently attracted many interests because of their response to environmental stimuli such as temperature.					
37245771	11	51	theme	viscoelastic	1790:1801	arg1	transition					1803:1812	a gel-sol viscoelastic transition	1780:1812	a gel-sol viscoelastic transition	1780:1812	Based on the rheological results, the produced thermo-responsive hydrogel shows a gel-sol viscoelastic transition at a temperature of 24.5 °C.					
37245771	15	52	theme	due	2331:2333	arg1	candidiasis					2319:2329	cutaneous candidiasis	2309:2329	cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems	2309:2454	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	2	53	used	used	366:369	arg2	hydrogels					334:342	Biomaterial-based hydrogels	316:342	Biomaterial-based hydrogels	316:342	Biomaterial-based hydrogels have been extensively used in biomedical applications, and they are promising platforms to encapsulate essential oils.					
37245771	14	54	theme	thermo-sensitive	2121:2136	arg1	formulation					2138:2148	the designed thermo-sensitive formulation	2108:2148	the designed thermo-sensitive formulation	2108:2148	In addition, 2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay demonstrates that the designed thermo-sensitive formulation is biocompatible with high cell viability (over 96 %).					
37245771	14	54	theme	thermo-sensitive	2121:2136	arg1	biocompatible					2153:2165	biocompatible	2153:2165	biocompatible	2153:2165	In addition, 2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay demonstrates that the designed thermo-sensitive formulation is biocompatible with high cell viability (over 96 %).					
37245771	11	55	theme	24.5 °C	1834:1840	arg1	temperature					1819:1829	a temperature	1817:1829	a temperature of 24.5 °C	1817:1840	Based on the rheological results, the produced thermo-responsive hydrogel shows a gel-sol viscoelastic transition at a temperature of 24.5 °C.					
37245771	9	56	theme	multiflora	1483:1492	arg1	oil					1504:1506	the Zataria multiflora essential oil	1471:1506	the Zataria multiflora essential oil	1471:1506	It is found that thymol (44.30 %) and γ-terpinene (22.62 %) are the main constituents of the Zataria multiflora essential oil.					
37245771	8	57	theme	fabricated	1251:1260	arg1	hydrogel					1262:1269	the fabricated hydrogel	1247:1269	the fabricated hydrogel	1247:1269	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	4	58	theme	alcohol/chitosan/gelatin	695:718	arg1	hydrogel					720:727	a polyvinyl alcohol/chitosan/gelatin hydrogel	683:727	a polyvinyl alcohol/chitosan/gelatin hydrogel	683:727	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	11	59	theme	rheological	1713:1723	arg1	results					1725:1731	the rheological results	1709:1731	the rheological results	1709:1731	Based on the rheological results, the produced thermo-responsive hydrogel shows a gel-sol viscoelastic transition at a temperature of 24.5 °C.					
37245771	9	60	theme	oil	1504:1506	arg1	thymol					1399:1404	thymol	1399:1404	thymol (44.30 %)	1399:1414	It is found that thymol (44.30 %) and γ-terpinene (22.62 %) are the main constituents of the Zataria multiflora essential oil.					
37245771	9	60	theme	oil	1504:1506	arg1	γ-terpinene					1420:1430	γ-terpinene	1420:1430	γ-terpinene (22.62 %)	1420:1440	It is found that thymol (44.30 %) and γ-terpinene (22.62 %) are the main constituents of the Zataria multiflora essential oil.					
37245771	9	60	theme	oil	1504:1506	arg1	constituents					1455:1466	the main constituents	1446:1466	the main constituents of the Zataria multiflora essential oil	1446:1506	It is found that thymol (44.30 %) and γ-terpinene (22.62 %) are the main constituents of the Zataria multiflora essential oil.					
37245771	12	61	theme	essential	1899:1907	arg1	oil					1909:1911	the loaded essential oil	1888:1911	the loaded essential oil	1888:1911	This transition leads to a facile release of the loaded essential oil.					
37245771	11	62	theme	produced	1738:1745	arg1	hydrogel					1765:1772	the produced thermo-responsive hydrogel	1734:1772	the produced thermo-responsive hydrogel	1734:1772	Based on the rheological results, the produced thermo-responsive hydrogel shows a gel-sol viscoelastic transition at a temperature of 24.5 °C.					
37245771	1	63	theme	delivery	297:304	arg1	platform					306:313	a delivery platform	295:313	a delivery platform	295:313	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	10	64	theme	oil	1669:1671	arg1	constituents					1673:1684	the essential oil constituents	1655:1684	the essential oil constituents	1655:1684	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37245771	14	65	with	biocompatible	2153:2165	arg1	viability					2182:2190	high cell viability	2172:2190	high cell viability (over 96 %)	2172:2202	In addition, 2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay demonstrates that the designed thermo-sensitive formulation is biocompatible with high cell viability (over 96 %).					
37245771	8	66	theme	chromatography-mass	1291:1309	arg1	spectroscopy					1311:1322	gas chromatography-mass spectroscopy	1287:1322	gas chromatography-mass spectroscopy (GC-MS)	1287:1330	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	8	66	theme	chromatography-mass	1291:1309	arg1	GC-MS					1325:1329	GC-MS	1325:1329	GC-MS	1325:1329	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	15	67	theme	intelligent	2258:2268	arg1	platform					2284:2291	a potential intelligent drug delivery platform	2246:2291	a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems	2246:2454	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	15	67	theme	intelligent	2258:2268	arg1	hydrogel					2220:2227	The fabricated hydrogel	2205:2227	The fabricated hydrogel	2205:2227	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	5	68	theme	SEM	951:953	arg1	results					963:969	the SEM imaging results	947:969	the SEM imaging results	947:969	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	5	69	with	consistent	931:940	arg1	results					963:969	the SEM imaging results	947:969	the SEM imaging results	947:969	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	4	70	theme	positive	734:741	arg1	oil					660:662	Zataria multiflora essential oil	631:662	Zataria multiflora essential oil	631:662	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	4	70	theme	positive	734:741	arg1	platform					776:783	a positive thermo-responsive and antifungal platform	732:783	a positive thermo-responsive and antifungal platform	732:783	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	5	71	theme	encapsulated	834:845	arg1	droplets					871:878	the encapsulated spherical essential oil droplets	830:878	the encapsulated spherical essential oil droplets	830:878	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	15	72	theme	fabricated	2209:2218	arg1	platform					2284:2291	a potential intelligent drug delivery platform	2246:2291	a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems	2246:2454	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	15	72	theme	fabricated	2209:2218	arg1	hydrogel					2220:2227	The fabricated hydrogel	2205:2227	The fabricated hydrogel	2205:2227	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	8	73	theme	infrared	1354:1361	arg1	techniques					1370:1379	infrared (FTIR) techniques	1354:1379	infrared (FTIR) techniques	1354:1379	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	15	74	theme	cutaneous	2309:2317	arg1	candidiasis					2319:2329	cutaneous candidiasis	2309:2329	cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems	2309:2454	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	5	75	theme	microscopic	811:821	arg1	image					823:827	the optical microscopic image	799:827	the optical microscopic image	799:827	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	2	76	theme	Biomaterial-based	316:332	arg1	hydrogels					334:342	Biomaterial-based hydrogels	316:342	Biomaterial-based hydrogels	316:342	Biomaterial-based hydrogels have been extensively used in biomedical applications, and they are promising platforms to encapsulate essential oils.					
37245771	7	77	theme	essential	1142:1150	arg1	oil					1152:1154	the Zataria multiflora essential oil	1119:1154	the Zataria multiflora essential oil	1119:1154	These results confirm the successful efficient encapsulation of the Zataria multiflora essential oil within the hydrogel.					
37245771	0	78	theme	thermo-responsive	88:104	arg1	Synthesis					116:124	polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis	53:124	polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis	53:124	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	15	79	theme	delivery	2439:2446	arg1	systems					2448:2454	traditional drug delivery systems	2422:2454	traditional drug delivery systems	2422:2454	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	7	80	theme	Zataria	1123:1129	arg1	oil					1152:1154	the Zataria multiflora essential oil	1119:1154	the Zataria multiflora essential oil	1119:1154	These results confirm the successful efficient encapsulation of the Zataria multiflora essential oil within the hydrogel.					
37245771	15	81	theme	delivery	2275:2282	arg1	platform					2284:2291	a potential intelligent drug delivery platform	2246:2291	a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems	2246:2454	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	15	81	theme	delivery	2275:2282	arg1	hydrogel					2220:2227	The fabricated hydrogel	2205:2227	The fabricated hydrogel	2205:2227	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	1	82	theme	therapeutic	262:272	arg1	applications					274:285	therapeutic applications	262:285	therapeutic applications	262:285	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	13	83	theme	multiflora	1966:1975	arg1	oil					1987:1989	Zataria multiflora essential oil	1958:1989	Zataria multiflora essential oil	1958:1989	The release test depicts that about 30 % of Zataria multiflora essential oil is released during the first 16 min.					
37245771	1	84	theme	multiflora	195:204	arg1	oil					216:218	Zataria multiflora essential oil	187:218	Zataria multiflora essential oil	187:218	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	1	84	theme	multiflora	195:204	arg1	product					248:254	a natural volatile plant product	223:254	a natural volatile plant product whose therapeutic applications require a delivery platform	223:313	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	13	85	theme	oil	1987:1989	arg1	oil					1987:1989	Zataria multiflora essential oil	1958:1989	Zataria multiflora essential oil	1958:1989	The release test depicts that about 30 % of Zataria multiflora essential oil is released during the first 16 min.					
37245771	13	85	theme	oil	1987:1989	arg1	%					1953:1953	about 30 %	1944:1953	about 30 % of Zataria multiflora essential oil	1944:1989	The release test depicts that about 30 % of Zataria multiflora essential oil is released during the first 16 min.					
37245771	5	86	theme	essential	857:865	arg1	droplets					871:878	the encapsulated spherical essential oil droplets	830:878	the encapsulated spherical essential oil droplets	830:878	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	7	87	theme	efficient	1092:1100	arg1	encapsulation					1102:1114	the successful efficient encapsulation	1077:1114	the successful efficient encapsulation of the Zataria multiflora essential oil within the hydrogel	1077:1174	These results confirm the successful efficient encapsulation of the Zataria multiflora essential oil within the hydrogel.					
37245771	15	88	theme	drug	2270:2273	arg1	platform					2284:2291	a potential intelligent drug delivery platform	2246:2291	a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems	2246:2454	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	15	88	theme	drug	2270:2273	arg1	hydrogel					2220:2227	The fabricated hydrogel	2205:2227	The fabricated hydrogel	2205:2227	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	1	89	theme	plant	242:246	arg1	oil					216:218	Zataria multiflora essential oil	187:218	Zataria multiflora essential oil	187:218	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	1	89	theme	plant	242:246	arg1	product					248:254	a natural volatile plant product	223:254	a natural volatile plant product whose therapeutic applications require a delivery platform	223:313	Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform.					
37245771	15	90	theme	traditional	2422:2432	arg1	systems					2448:2454	traditional drug delivery systems	2422:2454	traditional drug delivery systems	2422:2454	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	10	91	dep	albicans	1574:1581	arg1	biofilms					1583:1590	biofilms	1583:1590	Candida albicans biofilms (~60-80 %)	1566:1601	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37245771	10	91	dep	albicans	1574:1581	arg1	%					1600:1600	~60-80 %	1593:1600	~60-80 %	1593:1600	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37245771	4	92	theme	essential	650:658	arg1	oil					660:662	Zataria multiflora essential oil	631:662	Zataria multiflora essential oil	631:662	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	4	92	theme	essential	650:658	arg1	platform					776:783	a positive thermo-responsive and antifungal platform	732:783	a positive thermo-responsive and antifungal platform	732:783	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	10	93	theme	Candida	1566:1572	arg1	albicans					1574:1581	Candida albicans biofilms (~60-80 %)	1566:1601	Candida albicans biofilms (~60-80 %)	1566:1601	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37245771	2	94	theme	essential	447:455	arg1	oils					457:460	essential oils	447:460	essential oils	447:460	Biomaterial-based hydrogels have been extensively used in biomedical applications, and they are promising platforms to encapsulate essential oils.					
37245771	0	95	theme	multiflora	25:34	arg1	oil					46:48	Zataria multiflora essential oil	17:48	Zataria multiflora essential oil	17:48	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	14	96	theme	high	2172:2175	arg1	viability					2182:2190	high cell viability	2172:2190	high cell viability (over 96 %)	2172:2202	In addition, 2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay demonstrates that the designed thermo-sensitive formulation is biocompatible with high cell viability (over 96 %).					
37245771	5	97	theme	1.10 ± 0.64 μm	902:915	arg1	consistent					931:940	consistent	931:940	consistent	931:940	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	5	97	theme	1.10 ± 0.64 μm	902:915	arg1	size					894:897	a mean size	887:897	a mean size	887:897	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	9	98	theme	main	1450:1453	arg1	thymol					1399:1404	thymol	1399:1404	thymol (44.30 %)	1399:1414	It is found that thymol (44.30 %) and γ-terpinene (22.62 %) are the main constituents of the Zataria multiflora essential oil.					
37245771	9	98	theme	main	1450:1453	arg1	γ-terpinene					1420:1430	γ-terpinene	1420:1430	γ-terpinene (22.62 %)	1420:1440	It is found that thymol (44.30 %) and γ-terpinene (22.62 %) are the main constituents of the Zataria multiflora essential oil.					
37245771	9	98	theme	main	1450:1453	arg1	constituents					1455:1466	the main constituents	1446:1466	the main constituents of the Zataria multiflora essential oil	1446:1506	It is found that thymol (44.30 %) and γ-terpinene (22.62 %) are the main constituents of the Zataria multiflora essential oil.					
37245771	8	99	theme	oil	1239:1241	arg1	compositions					1190:1201	The chemical compositions	1177:1201	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel	1177:1269	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	14	100	theme	5-diphenyl-2H-tetrazolium	2044:2068	arg1	assay					2084:2088	2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay	2041:2088	assay	2084:2088	In addition, 2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay demonstrates that the designed thermo-sensitive formulation is biocompatible with high cell viability (over 96 %).					
37245771	3	101	theme	intelligent	490:500	arg1	hydrogels					502:510	intelligent hydrogels	490:510	intelligent hydrogels	490:510	Among different hydrogels, intelligent hydrogels have recently attracted many interests because of their response to environmental stimuli such as temperature.					
37245771	8	102	dep	infrared	1354:1361	arg1	FTIR					1364:1367	FTIR	1364:1367	FTIR	1364:1367	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	11	103	theme	gel-sol	1782:1788	arg1	transition					1803:1812	a gel-sol viscoelastic transition	1780:1812	a gel-sol viscoelastic transition	1780:1812	Based on the rheological results, the produced thermo-responsive hydrogel shows a gel-sol viscoelastic transition at a temperature of 24.5 °C.					
37245771	15	104	theme	promising	2397:2405	arg1	alternative					2407:2417	a promising alternative	2395:2417	a promising alternative to traditional drug delivery systems	2395:2454	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	15	104	theme	promising	2397:2405	arg1	effectiveness					2349:2361	antifungal effectiveness	2338:2361	antifungal effectiveness	2338:2361	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	15	104	theme	promising	2397:2405	arg1	toxicity					2372:2379	less toxicity	2367:2379	less toxicity	2367:2379	The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.					
37245771	0	105	theme	polyvinyl	53:61	arg1	Synthesis					116:124	polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis	53:124	polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis	53:124	Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations.					
37245771	6	106	theme	Encapsulation	972:984	arg1	efficacy					986:993	Encapsulation efficacy	972:993	Encapsulation efficacy	972:993	Encapsulation efficacy and loading capacity are 98.66 % and 12.98 %, respectively.					
37245771	6	106	theme	Encapsulation	972:984	arg1	capacity					1007:1014	loading capacity	999:1014	loading capacity	999:1014	Encapsulation efficacy and loading capacity are 98.66 % and 12.98 %, respectively.					
37245771	6	106	theme	Encapsulation	972:984	arg1	%					1026:1026	98.66 %	1020:1026	98.66 %	1020:1026	Encapsulation efficacy and loading capacity are 98.66 % and 12.98 %, respectively.					
37245771	12	107	theme	oil	1909:1911	arg1	release					1877:1883	a facile release	1868:1883	a facile release of the loaded essential oil	1868:1911	This transition leads to a facile release of the loaded essential oil.					
37245771	5	108	theme	spherical	847:855	arg1	droplets					871:878	the encapsulated spherical essential oil droplets	830:878	the encapsulated spherical essential oil droplets	830:878	According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results.					
37245771	4	109	theme	Zataria	631:637	arg1	oil					660:662	Zataria multiflora essential oil	631:662	Zataria multiflora essential oil	631:662	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	4	109	theme	Zataria	631:637	arg1	platform					776:783	a positive thermo-responsive and antifungal platform	732:783	a positive thermo-responsive and antifungal platform	732:783	Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform.					
37245771	8	110	theme	chemical	1181:1188	arg1	compositions					1190:1201	The chemical compositions	1177:1201	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel	1177:1269	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	3	111	theme	environmental	580:592	arg1	stimuli					594:600	environmental stimuli	580:600	environmental stimuli such as temperature	580:620	Among different hydrogels, intelligent hydrogels have recently attracted many interests because of their response to environmental stimuli such as temperature.					
37245771	3	111	theme	environmental	580:592	arg1	temperature					610:620	temperature	610:620	temperature	610:620	Among different hydrogels, intelligent hydrogels have recently attracted many interests because of their response to environmental stimuli such as temperature.					
37245771	9	112	theme	Zataria	1475:1481	arg1	oil					1504:1506	the Zataria multiflora essential oil	1471:1506	the Zataria multiflora essential oil	1471:1506	It is found that thymol (44.30 %) and γ-terpinene (22.62 %) are the main constituents of the Zataria multiflora essential oil.					
37245771	8	113	theme	hydrogel	1262:1269	arg1	compositions					1190:1201	The chemical compositions	1177:1201	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel	1177:1269	The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared (FTIR) techniques.					
37245771	14	114	theme	designed	2112:2119	arg1	formulation					2138:2148	the designed thermo-sensitive formulation	2108:2148	the designed thermo-sensitive formulation	2108:2148	In addition, 2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay demonstrates that the designed thermo-sensitive formulation is biocompatible with high cell viability (over 96 %).					
37245771	14	114	theme	designed	2112:2119	arg1	biocompatible					2153:2165	biocompatible	2153:2165	biocompatible	2153:2165	In addition, 2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay demonstrates that the designed thermo-sensitive formulation is biocompatible with high cell viability (over 96 %).					
37245771	9	115	theme	essential	1494:1502	arg1	oil					1504:1506	the Zataria multiflora essential oil	1471:1506	the Zataria multiflora essential oil	1471:1506	It is found that thymol (44.30 %) and γ-terpinene (22.62 %) are the main constituents of the Zataria multiflora essential oil.					
37245771	10	116	theme	antifungal	1632:1641	arg1	activity					1643:1650	the antifungal activity	1628:1650	the antifungal activity of the essential oil constituents and chitosan	1628:1697	The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (~60-80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan.					
37153993	18	0	theme	new	3153:3155	arg1	bone					3157:3160	new bone	3153:3160	new bone	3153:3160	Although dental pocket could be observed in the periodontal tissue of rats treated with chitosan oligosaccharide, it was not obvious, and new bone appeared around the alveolar bone.					
37153993	1	1	with	mice	231:234	arg1	periodontitis					258:270	periodontitis	258:270	periodontitis	258:270	PURPOSE To investigate the effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in mice with osteoporosis and periodontitis.					
37153993	1	1	with	mice	231:234	arg1	osteoporosis					241:252	osteoporosis	241:252	osteoporosis	241:252	PURPOSE To investigate the effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in mice with osteoporosis and periodontitis.					
37153993	16	2	theme	serum	2558:2562	arg1	density					2549:2555	the bone mineral density	2532:2555	the bone mineral density	2532:2555	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	16	2	theme	serum	2558:2562	arg1	ALP					2564:2566	serum ALP	2558:2566	serum ALP	2558:2566	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	19	3	theme	bone	3268:3271	arg1	metabolism					3273:3282	bone metabolism	3268:3282	bone metabolism	3268:3282	CONCLUSIONS Chitosan oligosaccharide can induce biochemical indexes of bone metabolism to become normal, alleviate the symptoms of periodontitis, this may be related to the inhibition of IKK/NF-κB pathway by chitosan oligosaccharide.					
37153993	19	4	theme	biochemical	3245:3255	arg1	indexes					3257:3263	biochemical indexes	3245:3263	biochemical indexes of bone metabolism	3245:3282	CONCLUSIONS Chitosan oligosaccharide can induce biochemical indexes of bone metabolism to become normal, alleviate the symptoms of periodontitis, this may be related to the inhibition of IKK/NF-κB pathway by chitosan oligosaccharide.					
37153993	16	5	theme	periodontal	2745:2755	arg1	tissue					2757:2762	periodontal tissue	2745:2762	periodontal tissue	2745:2762	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	0	6	with	rats	81:84	arg1	osteoporosis					91:102	osteoporosis	91:102	osteoporosis	91:102	[Effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in rats with osteoporosis and periodontitis].					
37153993	0	6	with	rats	81:84	arg1	periodontitis					108:120	periodontitis	108:120	periodontitis	108:120	[Effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in rats with osteoporosis and periodontitis].					
37153993	15	7	theme	protein	2404:2410	arg1	expression					2412:2421	protein expression	2404:2421	protein expression	2404:2421	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	8	theme	mineral	2186:2192	arg1	BGP					2214:2216	BGP	2214:2216	BGP	2214:2216	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	8	theme	mineral	2186:2192	arg1	density					2194:2200	the bone mineral density	2177:2200	the bone mineral density	2177:2200	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	8	theme	mineral	2186:2192	arg1	ALP					2209:2211	serum ALP	2203:2211	serum ALP	2203:2211	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	6	9	theme	periodontal	890:900	arg1	tissues					902:908	The periodontal tissues	886:908	The periodontal tissues of each group	886:922	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	16	10	theme	attachment	2654:2663	arg1	loss					2665:2668	periodontal attachment loss	2642:2668	periodontal attachment loss	2642:2668	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	15	11	theme	alveolar	2355:2362	arg1	resorption					2369:2378	alveolar bone resorption	2355:2378	alveolar bone resorption	2355:2378	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	10	12	theme	absorption	1691:1700	arg1	value					1702:1706	alveolar bone absorption value	1677:1706	alveolar bone absorption value	1677:1706	The maxilla was removed, and the distance from the enamel cementum boundary to the alveolar crest was measured to obtain alveolar bone absorption value.					
37153993	19	13	dep	induce	3238:3243	arg1	alleviate					3302:3310	alleviate	3302:3310	alleviate the symptoms of periodontitis	3302:3340	CONCLUSIONS Chitosan oligosaccharide can induce biochemical indexes of bone metabolism to become normal, alleviate the symptoms of periodontitis, this may be related to the inhibition of IKK/NF-κB pathway by chitosan oligosaccharide.					
37153993	19	14	theme	pathway	3394:3400	arg1	inhibition					3370:3379	the inhibition	3366:3379	the inhibition of IKK/NF-κB pathway by chitosan oligosaccharide	3366:3428	CONCLUSIONS Chitosan oligosaccharide can induce biochemical indexes of bone metabolism to become normal, alleviate the symptoms of periodontitis, this may be related to the inhibition of IKK/NF-κB pathway by chitosan oligosaccharide.					
37153993	15	15	from	index	2313:2317	arg1	tissue					2438:2443	periodontal tissue	2426:2443	periodontal tissue	2426:2443	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	19	16	theme	metabolism	3273:3282	arg1	indexes					3257:3263	biochemical indexes	3245:3263	biochemical indexes of bone metabolism	3245:3282	CONCLUSIONS Chitosan oligosaccharide can induce biochemical indexes of bone metabolism to become normal, alleviate the symptoms of periodontitis, this may be related to the inhibition of IKK/NF-κB pathway by chitosan oligosaccharide.					
37153993	10	17	theme	alveolar	1677:1684	arg1	value					1702:1706	alveolar bone absorption value	1677:1706	alveolar bone absorption value	1677:1706	The maxilla was removed, and the distance from the enamel cementum boundary to the alveolar crest was measured to obtain alveolar bone absorption value.					
37153993	1	18	theme	oligosaccharide	171:185	arg1	effects					151:157	the effects	147:157	the effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in mice with osteoporosis and periodontitis	147:270	PURPOSE To investigate the effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in mice with osteoporosis and periodontitis.					
37153993	16	19	theme	bone	2683:2686	arg1	resorption					2688:2697	alveolar bone resorption	2674:2697	alveolar bone resorption	2674:2697	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	15	20	from	loss	2320:2323	arg1	tissue					2438:2443	periodontal tissue	2426:2443	periodontal tissue	2426:2443	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	1	21	theme	bone	190:193	arg1	metabolism					195:204	bone metabolism	190:204	bone metabolism	190:204	PURPOSE To investigate the effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in mice with osteoporosis and periodontitis.					
37153993	14	22	dep	RESULTS	1953:1959	arg1	gums					2050:2053	the gums	2046:2053	the gums of the other two groups	2046:2077	RESULTS Before administration, the gums of the control group were pink without bleeding, and the gums of the other two groups were red and swollen with slight bleeding.					
37153993	14	22	dep	RESULTS	1953:1959	arg1	gums					1988:1991	the gums	1984:1991	the gums of the control group	1984:2012	RESULTS Before administration, the gums of the control group were pink without bleeding, and the gums of the other two groups were red and swollen with slight bleeding.					
37153993	14	22	dep	RESULTS	1953:1959	arg1	red					2084:2086	red	2084:2086	red	2084:2086	RESULTS Before administration, the gums of the control group were pink without bleeding, and the gums of the other two groups were red and swollen with slight bleeding.					
37153993	14	22	dep	RESULTS	1953:1959	arg1	pink					2019:2022	pink	2019:2022	pink	2019:2022	RESULTS Before administration, the gums of the control group were pink without bleeding, and the gums of the other two groups were red and swollen with slight bleeding.					
37153993	5	23	theme	treatment	719:727	arg1	group					729:733	chitosan oligosaccharide treatment group	694:733	chitosan oligosaccharide treatment group	694:733	Four weeks after ligation, the rats in chitosan oligosaccharide treatment group were gavaged with 200 mg/kg chitosan oligosaccharide, and the other two groups were gavaged with equal volume of normal saline once a day for 90 days.					
37153993	16	24	theme	ovariectomized	2496:2509	arg1	group					2525:2529	the ovariectomized periodontitis group	2492:2529	the ovariectomized periodontitis group	2492:2529	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	15	25	theme	periodontal	2328:2338	arg1	attachment					2340:2349	periodontal attachment	2328:2349	periodontal attachment	2328:2349	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	11	26	theme	maxilla	1759:1765	arg1	pathology					1746:1754	the pathology	1742:1754	the pathology of maxilla	1742:1765	H-E staining was used to observe the pathology of maxilla in each group.					
37153993	16	27	theme	IKK	2710:2712	arg1	mRNA					2714:2717	IKK mRNA	2710:2717	IKK mRNA	2710:2717	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	15	28	theme	periodontitis	2236:2248	arg1	group					2250:2254	ovariectomized periodontitis group	2221:2254	ovariectomized periodontitis group	2221:2254	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	0	29	theme	oligosaccharide	21:35	arg1	[Effects					0:7	[Effects	0:7	[Effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in rats with osteoporosis and periodontitis	0:120	[Effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in rats with osteoporosis and periodontitis].					
37153993	9	30	theme	gingival	1422:1429	arg1	index					1431:1435	gingival index	1422:1435	gingival index	1422:1435	The gingival index and periodontal attachment loss of rats in each group were obtained by visual examination and exploratory examination.					
37153993	8	31	theme	enzyme-linked	1381:1393	arg1	assay					1411:1415	enzyme-linked immunodeficient assay	1381:1415	enzyme-linked immunodeficient assay	1381:1415	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	8	32	theme	serum	1254:1258	arg1	ALP					1282:1284	ALP	1282:1284	ALP	1282:1284	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	8	32	theme	serum	1254:1258	arg1	phosphatase					1269:1279	serum alkaline phosphatase	1254:1279	serum alkaline phosphatase (ALP)	1254:1285	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	17	33	theme	ovariectomized	2810:2823	arg1	group					2839:2843	the ovariectomized periodontitis group	2806:2843	the ovariectomized periodontitis group	2806:2843	In the ovariectomized periodontitis group, the periodontal tissue combined with epithelium was separated from the tooth surface, the dental pocket was obvious and deep, and the height of alveolar bone decreased.					
37153993	1	34	from	metabolism	195:204	arg1	mice					231:234	mice	231:234	mice with osteoporosis and periodontitis	231:270	PURPOSE To investigate the effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in mice with osteoporosis and periodontitis.					
37153993	6	35	theme	group	918:922	arg1	tissues					902:908	The periodontal tissues	886:908	The periodontal tissues of each group	886:922	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	17	36	theme	periodontitis	2825:2837	arg1	group					2839:2843	the ovariectomized periodontitis group	2806:2843	the ovariectomized periodontitis group	2806:2843	In the ovariectomized periodontitis group, the periodontal tissue combined with epithelium was separated from the tooth surface, the dental pocket was obvious and deep, and the height of alveolar bone decreased.					
37153993	16	37	theme	gingival	2626:2633	arg1	index					2635:2639	gingival index	2626:2639	gingival index	2626:2639	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	8	38	theme	phosphatase	1269:1279	arg1	contents					1242:1249	the contents	1238:1249	the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b)	1238:1362	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	17	39	theme	alveolar	2990:2997	arg1	bone					2999:3002	alveolar bone	2990:3002	alveolar bone	2990:3002	In the ovariectomized periodontitis group, the periodontal tissue combined with epithelium was separated from the tooth surface, the dental pocket was obvious and deep, and the height of alveolar bone decreased.					
37153993	5	40	theme	chitosan	694:701	arg1	group					729:733	chitosan oligosaccharide treatment group	694:733	chitosan oligosaccharide treatment group	694:733	Four weeks after ligation, the rats in chitosan oligosaccharide treatment group were gavaged with 200 mg/kg chitosan oligosaccharide, and the other two groups were gavaged with equal volume of normal saline once a day for 90 days.					
37153993	19	41	theme	IKK/NF-κB	3384:3392	arg1	pathway					3394:3400	IKK/NF-κB pathway	3384:3400	IKK/NF-κB pathway	3384:3400	CONCLUSIONS Chitosan oligosaccharide can induce biochemical indexes of bone metabolism to become normal, alleviate the symptoms of periodontitis, this may be related to the inhibition of IKK/NF-κB pathway by chitosan oligosaccharide.					
37153993	9	42	theme	attachment	1453:1462	arg1	loss					1464:1467	periodontal attachment loss	1441:1467	periodontal attachment loss	1441:1467	The gingival index and periodontal attachment loss of rats in each group were obtained by visual examination and exploratory examination.					
37153993	0	43	theme	IKK/NF-κB	60:68	arg1	pathway					70:76	IKK/NF-κB pathway	60:76	IKK/NF-κB pathway	60:76	[Effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in rats with osteoporosis and periodontitis].					
37153993	0	44	theme	chitosan	12:19	arg1	oligosaccharide					21:35	chitosan oligosaccharide	12:35	chitosan oligosaccharide	12:35	[Effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in rats with osteoporosis and periodontitis].					
37153993	6	45	theme	rats	993:996	arg1	density					982:988	the bone mineral density	965:988	the bone mineral density of rats	965:996	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	15	46	from	mRNA	2395:2398	arg1	tissue					2438:2443	periodontal tissue	2426:2443	periodontal tissue	2426:2443	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	11	47	used	used	1726:1729	arg2	staining					1713:1720	H-E staining	1709:1720	H-E staining	1709:1720	H-E staining was used to observe the pathology of maxilla in each group.					
37153993	1	48	from	effects	151:157	arg1	metabolism					195:204	bone metabolism	190:204	bone metabolism	190:204	PURPOSE To investigate the effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in mice with osteoporosis and periodontitis.					
37153993	1	48	from	effects	151:157	arg1	pathway					220:226	IKK/NF-κB pathway	210:226	IKK/NF-κB pathway in mice with osteoporosis and periodontitis	210:270	PURPOSE To investigate the effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in mice with osteoporosis and periodontitis.					
37153993	16	49	from	expression	2731:2740	arg1	tissue					2757:2762	periodontal tissue	2745:2762	periodontal tissue	2745:2762	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	0	50	from	[Effects	0:7	arg1	metabolism					45:54	bone metabolism	40:54	bone metabolism	40:54	[Effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in rats with osteoporosis and periodontitis].					
37153993	0	50	from	[Effects	0:7	arg1	pathway					70:76	IKK/NF-κB pathway	60:76	IKK/NF-κB pathway	60:76	[Effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in rats with osteoporosis and periodontitis].					
37153993	9	51	from	loss	1464:1467	arg1	group					1485:1489	each group	1480:1489	each group	1480:1489	The gingival index and periodontal attachment loss of rats in each group were obtained by visual examination and exploratory examination.					
37153993	8	52	theme	Gla	1293:1295	arg1	protein					1297:1303	bone Gla protein	1288:1303	bone Gla protein (BGP)	1288:1309	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	8	52	theme	Gla	1293:1295	arg1	BGP					1306:1308	BGP	1306:1308	BGP	1306:1308	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	6	53	theme	bone	969:972	arg1	density					982:988	the bone mineral density	965:988	the bone mineral density of rats	965:996	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	0	54	from	metabolism	45:54	arg1	rats					81:84	rats	81:84	rats with osteoporosis and periodontitis	81:120	[Effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in rats with osteoporosis and periodontitis].					
37153993	9	55	dep	index	1431:1435	arg1	The					1418:1420	The	1418:1420	The	1418:1420	The gingival index and periodontal attachment loss of rats in each group were obtained by visual examination and exploratory examination.					
37153993	19	56	theme	chitosan	3405:3412	arg1	oligosaccharide					3414:3428	chitosan oligosaccharide	3405:3428	chitosan oligosaccharide	3405:3428	CONCLUSIONS Chitosan oligosaccharide can induce biochemical indexes of bone metabolism to become normal, alleviate the symptoms of periodontitis, this may be related to the inhibition of IKK/NF-κB pathway by chitosan oligosaccharide.					
37153993	5	57	theme	mg/kg	757:761	arg1	oligosaccharide					772:786	200 mg/kg chitosan oligosaccharide	753:786	200 mg/kg chitosan oligosaccharide	753:786	Four weeks after ligation, the rats in chitosan oligosaccharide treatment group were gavaged with 200 mg/kg chitosan oligosaccharide, and the other two groups were gavaged with equal volume of normal saline once a day for 90 days.					
37153993	8	58	link	enzyme-linked	1381:1393	arg1	assay					1411:1415	enzyme-linked immunodeficient assay	1381:1415	enzyme-linked immunodeficient assay	1381:1415	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	14	59	theme	control	2000:2006	arg1	group					2008:2012	the control group	1996:2012	the control group	1996:2012	RESULTS Before administration, the gums of the control group were pink without bleeding, and the gums of the other two groups were red and swollen with slight bleeding.					
37153993	3	60	theme	periodontitis	411:423	arg1	group					425:429	ovariectomized periodontitis group	396:429	ovariectomized periodontitis group	396:429	They were divided into control group, ovariectomized periodontitis group and chitosan oligosaccharide treatment group.					
37153993	15	61	theme	control	2162:2168	arg1	group					2170:2174	the control group	2158:2174	the control group	2158:2174	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	62	theme	bone	2364:2367	arg1	resorption					2369:2378	alveolar bone resorption	2355:2378	alveolar bone resorption	2355:2378	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	9	63	theme	visual	1508:1513	arg1	examination					1515:1525	visual examination	1508:1525	visual examination	1508:1525	The gingival index and periodontal attachment loss of rats in each group were obtained by visual examination and exploratory examination.					
37153993	6	64	theme	bone	1039:1042	arg1	density					1052:1058	dual energy X-ray animal bone mineral density	1014:1058	dual energy X-ray animal bone mineral density	1014:1058	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	5	65	dep	weeks	660:664	arg1	ligation					672:679	ligation	672:679	ligation	672:679	Four weeks after ligation, the rats in chitosan oligosaccharide treatment group were gavaged with 200 mg/kg chitosan oligosaccharide, and the other two groups were gavaged with equal volume of normal saline once a day for 90 days.					
37153993	8	66	theme	tartrate	1315:1322	arg1	TRACP5b					1355:1361	TRACP5b	1355:1361	TRACP5b	1355:1361	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	8	66	theme	tartrate	1315:1322	arg1	5b					1351:1352	tartrate resistant acid phosphatase 5b	1315:1352	tartrate resistant acid phosphatase 5b (TRACP5b)	1315:1362	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	7	67	theme	mineral	1140:1146	arg1	density					1148:1154	the bone mineral density	1131:1154	the bone mineral density	1131:1154	After 90 days of administration, the bone mineral density was detected again.					
37153993	18	68	theme	periodontal	3063:3073	arg1	tissue					3075:3080	the periodontal tissue	3059:3080	the periodontal tissue of rats treated with chitosan oligosaccharide	3059:3126	Although dental pocket could be observed in the periodontal tissue of rats treated with chitosan oligosaccharide, it was not obvious, and new bone appeared around the alveolar bone.					
37153993	6	69	theme	X-ray	1026:1030	arg1	density					1052:1058	dual energy X-ray animal bone mineral density	1014:1058	dual energy X-ray animal bone mineral density	1014:1058	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	10	70	theme	enamel	1607:1612	arg1	boundary					1623:1630	the enamel cementum boundary	1603:1630	the enamel cementum boundary	1603:1630	The maxilla was removed, and the distance from the enamel cementum boundary to the alveolar crest was measured to obtain alveolar bone absorption value.					
37153993	8	71	theme	acid	1334:1337	arg1	TRACP5b					1355:1361	TRACP5b	1355:1361	TRACP5b	1355:1361	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	8	71	theme	acid	1334:1337	arg1	5b					1351:1352	tartrate resistant acid phosphatase 5b	1315:1352	tartrate resistant acid phosphatase 5b (TRACP5b)	1315:1362	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	3	72	theme	oligosaccharide	444:458	arg1	group					470:474	chitosan oligosaccharide treatment group	435:474	chitosan oligosaccharide treatment group	435:474	They were divided into control group, ovariectomized periodontitis group and chitosan oligosaccharide treatment group.					
37153993	5	73	theme	other	797:801	arg1	groups					807:812	the other two groups	793:812	the other two groups	793:812	Four weeks after ligation, the rats in chitosan oligosaccharide treatment group were gavaged with 200 mg/kg chitosan oligosaccharide, and the other two groups were gavaged with equal volume of normal saline once a day for 90 days.					
37153993	15	74	from	TRACP5b	2295:2301	arg1	tissue					2438:2443	periodontal tissue	2426:2443	periodontal tissue	2426:2443	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	6	75	theme	dual	1014:1017	arg1	density					1052:1058	dual energy X-ray animal bone mineral density	1014:1058	dual energy X-ray animal bone mineral density	1014:1058	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	3	76	theme	control	381:387	arg1	group					389:393	control group	381:393	control group	381:393	They were divided into control group, ovariectomized periodontitis group and chitosan oligosaccharide treatment group.					
37153993	8	77	theme	5b	1351:1352	arg1	contents					1242:1249	the contents	1238:1249	the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b)	1238:1362	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	7	78	theme	administration	1115:1128	arg1	days					1107:1110	90 days	1104:1110	90 days of administration	1104:1128	After 90 days of administration, the bone mineral density was detected again.					
37153993	15	79	theme	serum	2203:2207	arg1	density					2194:2200	the bone mineral density	2177:2200	the bone mineral density	2177:2200	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	79	theme	serum	2203:2207	arg1	ALP					2209:2211	serum ALP	2203:2211	serum ALP	2203:2211	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	5	80	theme	equal	832:836	arg1	volume					838:843	equal volume	832:843	equal volume of normal saline	832:860	Four weeks after ligation, the rats in chitosan oligosaccharide treatment group were gavaged with 200 mg/kg chitosan oligosaccharide, and the other two groups were gavaged with equal volume of normal saline once a day for 90 days.					
37153993	13	81	theme	software	1901:1908	arg1	package					1910:1916	SPSS 22.0 software package	1891:1916	SPSS 22.0 software package	1891:1916	SPSS 22.0 software package was used for statistical analysis.					
37153993	12	82	used	used	1811:1814	arg2	blot					1801:1804	Western blot	1793:1804	Western blot	1793:1804	RT-PCR and Western blot were used to detect the nuclear factors in periodontal tissue of rats in each group.					
37153993	12	82	used	used	1811:1814	arg2	RT-PCR					1782:1787	RT-PCR	1782:1787	RT-PCR	1782:1787	RT-PCR and Western blot were used to detect the nuclear factors in periodontal tissue of rats in each group.					
37153993	16	83	from	NF-κB	2700:2704	arg1	tissue					2757:2762	periodontal tissue	2745:2762	periodontal tissue	2745:2762	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	10	84	from	boundary	1623:1630	arg1	distance					1589:1596	the distance	1585:1596	the distance from the enamel cementum boundary to the alveolar crest	1585:1652	The maxilla was removed, and the distance from the enamel cementum boundary to the alveolar crest was measured to obtain alveolar bone absorption value.					
37153993	6	85	theme	analysis	1081:1088	arg1	system					1090:1095	analysis system	1081:1095	analysis system	1081:1095	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	17	86	theme	tooth	2917:2921	arg1	surface					2923:2929	the tooth surface	2913:2929	the tooth surface	2913:2929	In the ovariectomized periodontitis group, the periodontal tissue combined with epithelium was separated from the tooth surface, the dental pocket was obvious and deep, and the height of alveolar bone decreased.					
37153993	18	87	located	observed	3047:3054	arg1	tissue					3075:3080	the periodontal tissue	3059:3080	the periodontal tissue of rats treated with chitosan oligosaccharide	3059:3126	Although dental pocket could be observed in the periodontal tissue of rats treated with chitosan oligosaccharide, it was not obvious, and new bone appeared around the alveolar bone.					
37153993	18	87	located	observed	3047:3054	arg2	pocket					3031:3036	dental pocket	3024:3036	dental pocket	3024:3036	Although dental pocket could be observed in the periodontal tissue of rats treated with chitosan oligosaccharide, it was not obvious, and new bone appeared around the alveolar bone.					
37153993	15	88	theme	periodontal	2426:2436	arg1	tissue					2438:2443	periodontal tissue	2426:2443	periodontal tissue	2426:2443	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	89	theme	bone	2181:2184	arg1	BGP					2214:2216	BGP	2214:2216	BGP	2214:2216	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	89	theme	bone	2181:2184	arg1	density					2194:2200	the bone mineral density	2177:2200	the bone mineral density	2177:2200	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	89	theme	bone	2181:2184	arg1	ALP					2209:2211	serum ALP	2203:2211	serum ALP	2203:2211	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	6	90	theme	body	1064:1067	arg1	composition					1069:1079	body composition	1064:1079	body composition	1064:1079	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	4	91	theme	gingivalis	579:588	arg1	fluid					590:594	Porphyromonas gingivalis fluid	565:594	Porphyromonas gingivalis fluid	565:594	Except for the control group, the other two groups were ovariectomized and smeared with Porphyromonas gingivalis fluid to establish the model of osteoporosis with periodontitis.					
37153993	15	92	dep	increased	2445:2453	arg1	P＜0.05					2469:2474	P＜0.05	2469:2474	P＜0.05	2469:2474	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	17	93	theme	periodontal	2850:2860	arg1	tissue					2862:2867	the periodontal tissue	2846:2867	the periodontal tissue combined with epithelium	2846:2892	In the ovariectomized periodontitis group, the periodontal tissue combined with epithelium was separated from the tooth surface, the dental pocket was obvious and deep, and the height of alveolar bone decreased.					
37153993	2	94	from	rats	338:341	arg1	group					351:355	each group	346:355	each group	346:355	METHODS Thirty rats were randomly divided into 3 groups, with 10 rats in each group.					
37153993	16	95	theme	periodontal	2642:2652	arg1	loss					2665:2668	periodontal attachment loss	2642:2668	periodontal attachment loss	2642:2668	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	17	96	theme	dental	2936:2941	arg1	obvious					2954:2960	obvious	2954:2960	obvious	2954:2960	In the ovariectomized periodontitis group, the periodontal tissue combined with epithelium was separated from the tooth surface, the dental pocket was obvious and deep, and the height of alveolar bone decreased.					
37153993	17	96	theme	dental	2936:2941	arg1	pocket					2943:2948	the dental pocket	2932:2948	the dental pocket	2932:2948	In the ovariectomized periodontitis group, the periodontal tissue combined with epithelium was separated from the tooth surface, the dental pocket was obvious and deep, and the height of alveolar bone decreased.					
37153993	13	97	theme	SPSS	1891:1894	arg1	package					1910:1916	SPSS 22.0 software package	1891:1916	SPSS 22.0 software package	1891:1916	SPSS 22.0 software package was used for statistical analysis.					
37153993	10	98	theme	bone	1686:1689	arg1	value					1702:1706	alveolar bone absorption value	1677:1706	alveolar bone absorption value	1677:1706	The maxilla was removed, and the distance from the enamel cementum boundary to the alveolar crest was measured to obtain alveolar bone absorption value.					
37153993	1	99	theme	chitosan	162:169	arg1	oligosaccharide					171:185	chitosan oligosaccharide	162:185	chitosan oligosaccharide	162:185	PURPOSE To investigate the effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in mice with osteoporosis and periodontitis.					
37153993	14	100	theme	groups	2072:2077	arg1	gums					2050:2053	the gums	2046:2053	the gums of the other two groups	2046:2077	RESULTS Before administration, the gums of the control group were pink without bleeding, and the gums of the other two groups were red and swollen with slight bleeding.					
37153993	14	100	theme	groups	2072:2077	arg1	red					2084:2086	red	2084:2086	red	2084:2086	RESULTS Before administration, the gums of the control group were pink without bleeding, and the gums of the other two groups were red and swollen with slight bleeding.					
37153993	4	101	with	ovariectomized	533:546	arg1	fluid					590:594	Porphyromonas gingivalis fluid	565:594	Porphyromonas gingivalis fluid	565:594	Except for the control group, the other two groups were ovariectomized and smeared with Porphyromonas gingivalis fluid to establish the model of osteoporosis with periodontitis.					
37153993	16	102	dep	increased	2592:2600	arg1	P＜0.05					2602:2607	P＜0.05	2602:2607	P＜0.05	2602:2607	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	9	103	from	index	1431:1435	arg1	group					1485:1489	each group	1480:1489	each group	1480:1489	The gingival index and periodontal attachment loss of rats in each group were obtained by visual examination and exploratory examination.					
37153993	16	104	theme	alveolar	2674:2681	arg1	resorption					2688:2697	alveolar bone resorption	2674:2697	alveolar bone resorption	2674:2697	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	8	105	theme	tail	1223:1226	arg1	vein					1228:1231	tail vein	1223:1231	tail vein	1223:1231	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	14	106	theme	other	2062:2066	arg1	groups					2072:2077	the other two groups	2058:2077	the other two groups	2058:2077	RESULTS Before administration, the gums of the control group were pink without bleeding, and the gums of the other two groups were red and swollen with slight bleeding.					
37153993	5	107	theme	oligosaccharide	703:717	arg1	group					729:733	chitosan oligosaccharide treatment group	694:733	chitosan oligosaccharide treatment group	694:733	Four weeks after ligation, the rats in chitosan oligosaccharide treatment group were gavaged with 200 mg/kg chitosan oligosaccharide, and the other two groups were gavaged with equal volume of normal saline once a day for 90 days.					
37153993	18	108	theme	chitosan	3103:3110	arg1	oligosaccharide					3112:3126	chitosan oligosaccharide	3103:3126	chitosan oligosaccharide	3103:3126	Although dental pocket could be observed in the periodontal tissue of rats treated with chitosan oligosaccharide, it was not obvious, and new bone appeared around the alveolar bone.					
37153993	10	109	theme	alveolar	1639:1646	arg1	crest					1648:1652	the alveolar crest	1635:1652	the alveolar crest	1635:1652	The maxilla was removed, and the distance from the enamel cementum boundary to the alveolar crest was measured to obtain alveolar bone absorption value.					
37153993	5	110	theme	normal	848:853	arg1	saline					855:860	normal saline	848:860	normal saline	848:860	Four weeks after ligation, the rats in chitosan oligosaccharide treatment group were gavaged with 200 mg/kg chitosan oligosaccharide, and the other two groups were gavaged with equal volume of normal saline once a day for 90 days.					
37153993	0	111	from	pathway	70:76	arg1	rats					81:84	rats	81:84	rats with osteoporosis and periodontitis	81:120	[Effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in rats with osteoporosis and periodontitis].					
37153993	13	112	used	used	1922:1925	arg2	package					1910:1916	SPSS 22.0 software package	1891:1916	SPSS 22.0 software package	1891:1916	SPSS 22.0 software package was used for statistical analysis.					
37153993	16	113	theme	protein	2723:2729	arg1	expression					2731:2740	protein expression	2723:2740	protein expression	2723:2740	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	15	114	theme	group	2250:2254	arg1	BGP					2214:2216	BGP	2214:2216	BGP	2214:2216	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	114	theme	group	2250:2254	arg1	density					2194:2200	the bone mineral density	2177:2200	the bone mineral density	2177:2200	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	114	theme	group	2250:2254	arg1	ALP					2209:2211	serum ALP	2203:2211	serum ALP	2203:2211	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	4	115	with	model	613:617	arg1	periodontitis					640:652	periodontitis	640:652	periodontitis	640:652	Except for the control group, the other two groups were ovariectomized and smeared with Porphyromonas gingivalis fluid to establish the model of osteoporosis with periodontitis.					
37153993	1	116	from	pathway	220:226	arg1	mice					231:234	mice	231:234	mice with osteoporosis and periodontitis	231:270	PURPOSE To investigate the effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in mice with osteoporosis and periodontitis.					
37153993	12	117	theme	rats	1871:1874	arg1	tissue					1861:1866	periodontal tissue	1849:1866	periodontal tissue of rats	1849:1874	RT-PCR and Western blot were used to detect the nuclear factors in periodontal tissue of rats in each group.					
37153993	16	118	theme	periodontitis	2511:2523	arg1	group					2525:2529	the ovariectomized periodontitis group	2492:2529	the ovariectomized periodontitis group	2492:2529	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	15	119	theme	ovariectomized	2221:2234	arg1	group					2250:2254	ovariectomized periodontitis group	2221:2254	ovariectomized periodontitis group	2221:2254	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	2	120	theme	METHODS	273:279	arg1	rats					288:291	METHODS Thirty rats	273:291	METHODS Thirty rats	273:291	METHODS Thirty rats were randomly divided into 3 groups, with 10 rats in each group.					
37153993	15	121	theme	attachment	2340:2349	arg1	index					2313:2317	gingival index	2304:2317	gingival index	2304:2317	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	121	theme	attachment	2340:2349	arg1	mRNA					2395:2398	IKK mRNA	2391:2398	IKK mRNA	2391:2398	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	121	theme	attachment	2340:2349	arg1	loss					2320:2323	loss	2320:2323	loss of periodontal attachment and alveolar bone resorption	2320:2378	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	121	theme	attachment	2340:2349	arg1	TRACP5b					2295:2301	TRACP5b	2295:2301	TRACP5b	2295:2301	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	121	theme	attachment	2340:2349	arg1	NF-κB					2381:2385	NF-κB	2381:2385	NF-κB	2381:2385	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	121	theme	attachment	2340:2349	arg1	expression					2412:2421	protein expression	2404:2421	protein expression	2404:2421	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	9	122	theme	periodontal	1441:1451	arg1	loss					1464:1467	periodontal attachment loss	1441:1467	periodontal attachment loss	1441:1467	The gingival index and periodontal attachment loss of rats in each group were obtained by visual examination and exploratory examination.					
37153993	16	123	from	mRNA	2714:2717	arg1	tissue					2757:2762	periodontal tissue	2745:2762	periodontal tissue	2745:2762	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	8	124	theme	alkaline	1260:1267	arg1	ALP					1282:1284	ALP	1282:1284	ALP	1282:1284	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	8	124	theme	alkaline	1260:1267	arg1	phosphatase					1269:1279	serum alkaline phosphatase	1254:1279	serum alkaline phosphatase (ALP)	1254:1285	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	17	125	theme	bone	2999:3002	arg1	height					2980:2985	the height	2976:2985	the height of alveolar bone	2976:3002	In the ovariectomized periodontitis group, the periodontal tissue combined with epithelium was separated from the tooth surface, the dental pocket was obvious and deep, and the height of alveolar bone decreased.					
37153993	5	126	theme	chitosan	763:770	arg1	oligosaccharide					772:786	200 mg/kg chitosan oligosaccharide	753:786	200 mg/kg chitosan oligosaccharide	753:786	Four weeks after ligation, the rats in chitosan oligosaccharide treatment group were gavaged with 200 mg/kg chitosan oligosaccharide, and the other two groups were gavaged with equal volume of normal saline once a day for 90 days.					
37153993	15	127	from	NF-κB	2381:2385	arg1	tissue					2438:2443	periodontal tissue	2426:2443	periodontal tissue	2426:2443	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	128	dep	decreased	2256:2264	arg1	P＜0.05					2280:2285	P＜0.05	2280:2285	P＜0.05	2280:2285	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	14	129	with	red	2084:2086	arg1	bleeding					2112:2119	bleeding	2112:2119	bleeding	2112:2119	RESULTS Before administration, the gums of the control group were pink without bleeding, and the gums of the other two groups were red and swollen with slight bleeding.					
37153993	15	130	theme	gingival	2304:2311	arg1	index					2313:2317	gingival index	2304:2317	gingival index	2304:2317	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	9	131	theme	rats	1472:1475	arg1	index					1431:1435	gingival index	1422:1435	gingival index	1422:1435	The gingival index and periodontal attachment loss of rats in each group were obtained by visual examination and exploratory examination.					
37153993	9	131	theme	rats	1472:1475	arg1	loss					1464:1467	periodontal attachment loss	1441:1467	periodontal attachment loss	1441:1467	The gingival index and periodontal attachment loss of rats in each group were obtained by visual examination and exploratory examination.					
37153993	4	132	theme	other	511:515	arg1	ovariectomized					533:546	ovariectomized	533:546	ovariectomized	533:546	Except for the control group, the other two groups were ovariectomized and smeared with Porphyromonas gingivalis fluid to establish the model of osteoporosis with periodontitis.					
37153993	4	132	theme	other	511:515	arg1	groups					521:526	the other two groups	507:526	the other two groups	507:526	Except for the control group, the other two groups were ovariectomized and smeared with Porphyromonas gingivalis fluid to establish the model of osteoporosis with periodontitis.					
37153993	16	133	dep	decreased	2783:2791	arg1	P＜0.05					2794:2799	P＜0.05	2794:2799	P＜0.05	2794:2799	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	12	134	theme	periodontal	1849:1859	arg1	tissue					1861:1866	periodontal tissue	1849:1866	periodontal tissue of rats	1849:1874	RT-PCR and Western blot were used to detect the nuclear factors in periodontal tissue of rats in each group.					
37153993	19	135	theme	periodontitis	3328:3340	arg1	symptoms					3316:3323	the symptoms	3312:3323	the symptoms of periodontitis	3312:3340	CONCLUSIONS Chitosan oligosaccharide can induce biochemical indexes of bone metabolism to become normal, alleviate the symptoms of periodontitis, this may be related to the inhibition of IKK/NF-κB pathway by chitosan oligosaccharide.					
37153993	6	136	theme	mineral	974:980	arg1	density					982:988	the bone mineral density	965:988	the bone mineral density of rats	965:996	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	15	137	from	expression	2412:2421	arg1	tissue					2438:2443	periodontal tissue	2426:2443	periodontal tissue	2426:2443	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	8	138	theme	bone	1288:1291	arg1	protein					1297:1303	bone Gla protein	1288:1303	bone Gla protein (BGP)	1288:1309	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	8	138	theme	bone	1288:1291	arg1	BGP					1306:1308	BGP	1306:1308	BGP	1306:1308	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	11	139	theme	H-E	1709:1711	arg1	staining					1713:1720	H-E staining	1709:1720	H-E staining	1709:1720	H-E staining was used to observe the pathology of maxilla in each group.					
37153993	5	140	theme	200	753:755	arg1	mg/kg					757:761	mg/kg	757:761	mg/kg	757:761	Four weeks after ligation, the rats in chitosan oligosaccharide treatment group were gavaged with 200 mg/kg chitosan oligosaccharide, and the other two groups were gavaged with equal volume of normal saline once a day for 90 days.					
37153993	15	141	theme	IKK	2391:2393	arg1	mRNA					2395:2398	IKK mRNA	2391:2398	IKK mRNA	2391:2398	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	4	142	theme	control	492:498	arg1	group					500:504	the control group	488:504	the control group	488:504	Except for the control group, the other two groups were ovariectomized and smeared with Porphyromonas gingivalis fluid to establish the model of osteoporosis with periodontitis.					
37153993	14	143	theme	group	2008:2012	arg1	gums					1988:1991	the gums	1984:1991	the gums of the control group	1984:2012	RESULTS Before administration, the gums of the control group were pink without bleeding, and the gums of the other two groups were red and swollen with slight bleeding.					
37153993	14	143	theme	group	2008:2012	arg1	pink					2019:2022	pink	2019:2022	pink	2019:2022	RESULTS Before administration, the gums of the control group were pink without bleeding, and the gums of the other two groups were red and swollen with slight bleeding.					
37153993	8	144	theme	protein	1297:1303	arg1	contents					1242:1249	the contents	1238:1249	the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b)	1238:1362	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	3	145	theme	chitosan	435:442	arg1	group					470:474	chitosan oligosaccharide treatment group	435:474	chitosan oligosaccharide treatment group	435:474	They were divided into control group, ovariectomized periodontitis group and chitosan oligosaccharide treatment group.					
37153993	18	146	theme	rats	3085:3088	arg1	tissue					3075:3080	the periodontal tissue	3059:3080	the periodontal tissue of rats treated with chitosan oligosaccharide	3059:3126	Although dental pocket could be observed in the periodontal tissue of rats treated with chitosan oligosaccharide, it was not obvious, and new bone appeared around the alveolar bone.					
37153993	6	147	theme	mineral	1044:1050	arg1	density					1052:1058	dual energy X-ray animal bone mineral density	1014:1058	dual energy X-ray animal bone mineral density	1014:1058	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	7	148	theme	bone	1135:1138	arg1	density					1148:1154	the bone mineral density	1131:1154	the bone mineral density	1131:1154	After 90 days of administration, the bone mineral density was detected again.					
37153993	12	149	theme	Western	1793:1799	arg1	blot					1801:1804	Western blot	1793:1804	Western blot	1793:1804	RT-PCR and Western blot were used to detect the nuclear factors in periodontal tissue of rats in each group.					
37153993	15	150	theme	resorption	2369:2378	arg1	index					2313:2317	gingival index	2304:2317	gingival index	2304:2317	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	150	theme	resorption	2369:2378	arg1	mRNA					2395:2398	IKK mRNA	2391:2398	IKK mRNA	2391:2398	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	150	theme	resorption	2369:2378	arg1	loss					2320:2323	loss	2320:2323	loss of periodontal attachment and alveolar bone resorption	2320:2378	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	150	theme	resorption	2369:2378	arg1	TRACP5b					2295:2301	TRACP5b	2295:2301	TRACP5b	2295:2301	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	150	theme	resorption	2369:2378	arg1	NF-κB					2381:2385	NF-κB	2381:2385	NF-κB	2381:2385	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	15	150	theme	resorption	2369:2378	arg1	expression					2412:2421	protein expression	2404:2421	protein expression	2404:2421	After administration, compared with the control group, the bone mineral density, serum ALP, BGP of ovariectomized periodontitis group decreased significantly(P＜0.05); while TRACP5b, gingival index, loss of periodontal attachment and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue increased significantly(P＜0.05).					
37153993	6	151	theme	animal	1032:1037	arg1	density					1052:1058	dual energy X-ray animal bone mineral density	1014:1058	dual energy X-ray animal bone mineral density	1014:1058	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	18	152	theme	alveolar	3182:3189	arg1	bone					3191:3194	the alveolar bone	3178:3194	the alveolar bone	3178:3194	Although dental pocket could be observed in the periodontal tissue of rats treated with chitosan oligosaccharide, it was not obvious, and new bone appeared around the alveolar bone.					
37153993	4	153	theme	Porphyromonas	565:577	arg1	gingivalis					579:588	Porphyromonas gingivalis	565:588	Porphyromonas gingivalis fluid	565:594	Except for the control group, the other two groups were ovariectomized and smeared with Porphyromonas gingivalis fluid to establish the model of osteoporosis with periodontitis.					
37153993	6	154	dep	density	1052:1058	arg1	system					1090:1095	analysis system	1081:1095	analysis system	1081:1095	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	10	155	theme	cementum	1614:1621	arg1	boundary					1623:1630	the enamel cementum boundary	1603:1630	the enamel cementum boundary	1603:1630	The maxilla was removed, and the distance from the enamel cementum boundary to the alveolar crest was measured to obtain alveolar bone absorption value.					
37153993	0	156	theme	bone	40:43	arg1	metabolism					45:54	bone metabolism	40:54	bone metabolism	40:54	[Effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in rats with osteoporosis and periodontitis].					
37153993	1	157	theme	IKK/NF-κB	210:218	arg1	pathway					220:226	IKK/NF-κB pathway	210:226	IKK/NF-κB pathway in mice with osteoporosis and periodontitis	210:270	PURPOSE To investigate the effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in mice with osteoporosis and periodontitis.					
37153993	9	158	theme	exploratory	1531:1541	arg1	examination					1543:1553	exploratory examination	1531:1553	exploratory examination	1531:1553	The gingival index and periodontal attachment loss of rats in each group were obtained by visual examination and exploratory examination.					
37153993	6	159	theme	energy	1019:1024	arg1	density					1052:1058	dual energy X-ray animal bone mineral density	1014:1058	dual energy X-ray animal bone mineral density	1014:1058	The periodontal tissues of each group were observed before administration, and the bone mineral density of rats was detected by dual energy X-ray animal bone mineral density and body composition analysis system.					
37153993	3	160	theme	ovariectomized	396:409	arg1	group					425:429	ovariectomized periodontitis group	396:429	ovariectomized periodontitis group	396:429	They were divided into control group, ovariectomized periodontitis group and chitosan oligosaccharide treatment group.					
37153993	8	161	theme	resistant	1324:1332	arg1	TRACP5b					1355:1361	TRACP5b	1355:1361	TRACP5b	1355:1361	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	8	161	theme	resistant	1324:1332	arg1	5b					1351:1352	tartrate resistant acid phosphatase 5b	1315:1352	tartrate resistant acid phosphatase 5b (TRACP5b)	1315:1362	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	3	162	theme	treatment	460:468	arg1	group					470:474	chitosan oligosaccharide treatment group	435:474	chitosan oligosaccharide treatment group	435:474	They were divided into control group, ovariectomized periodontitis group and chitosan oligosaccharide treatment group.					
37153993	8	163	theme	phosphatase	1339:1349	arg1	TRACP5b					1355:1361	TRACP5b	1355:1361	TRACP5b	1355:1361	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	8	163	theme	phosphatase	1339:1349	arg1	5b					1351:1352	tartrate resistant acid phosphatase 5b	1315:1352	tartrate resistant acid phosphatase 5b (TRACP5b)	1315:1362	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	13	164	theme	statistical	1931:1941	arg1	analysis					1943:1950	statistical analysis	1931:1950	statistical analysis	1931:1950	SPSS 22.0 software package was used for statistical analysis.					
37153993	8	165	theme	immunodeficient	1395:1409	arg1	assay					1411:1415	enzyme-linked immunodeficient assay	1381:1415	enzyme-linked immunodeficient assay	1381:1415	After administration, blood was collected from tail vein, and the contents of serum alkaline phosphatase (ALP), bone Gla protein (BGP) and tartrate resistant acid phosphatase 5b (TRACP5b) were measured by enzyme-linked immunodeficient assay.					
37153993	16	166	theme	bone	2536:2539	arg1	BGP					2569:2571	BGP	2569:2571	BGP	2569:2571	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	16	166	theme	bone	2536:2539	arg1	density					2549:2555	the bone mineral density	2532:2555	the bone mineral density	2532:2555	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	16	166	theme	bone	2536:2539	arg1	ALP					2564:2566	serum ALP	2558:2566	serum ALP	2558:2566	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	19	167	theme	Chitosan	3209:3216	arg1	oligosaccharide					3218:3232	Chitosan oligosaccharide	3209:3232	Chitosan oligosaccharide	3209:3232	CONCLUSIONS Chitosan oligosaccharide can induce biochemical indexes of bone metabolism to become normal, alleviate the symptoms of periodontitis, this may be related to the inhibition of IKK/NF-κB pathway by chitosan oligosaccharide.					
37153993	12	168	theme	nuclear	1830:1836	arg1	factors					1838:1844	the nuclear factors	1826:1844	the nuclear factors	1826:1844	RT-PCR and Western blot were used to detect the nuclear factors in periodontal tissue of rats in each group.					
37153993	16	169	theme	mineral	2541:2547	arg1	BGP					2569:2571	BGP	2569:2571	BGP	2569:2571	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	16	169	theme	mineral	2541:2547	arg1	density					2549:2555	the bone mineral density	2532:2555	the bone mineral density	2532:2555	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	16	169	theme	mineral	2541:2547	arg1	ALP					2564:2566	serum ALP	2558:2566	serum ALP	2558:2566	Compared with the ovariectomized periodontitis group, the bone mineral density, serum ALP, BGP were significantly increased(P＜0.05); while TRACP5b, gingival index, periodontal attachment loss and alveolar bone resorption, NF-κB and IKK mRNA and protein expression in periodontal tissue were significantly decreased (P＜0.05).					
37153993	5	170	theme	saline	855:860	arg1	volume					838:843	equal volume	832:843	equal volume of normal saline	832:860	Four weeks after ligation, the rats in chitosan oligosaccharide treatment group were gavaged with 200 mg/kg chitosan oligosaccharide, and the other two groups were gavaged with equal volume of normal saline once a day for 90 days.					
37153993	18	171	theme	dental	3024:3029	arg1	pocket					3031:3036	dental pocket	3024:3036	dental pocket	3024:3036	Although dental pocket could be observed in the periodontal tissue of rats treated with chitosan oligosaccharide, it was not obvious, and new bone appeared around the alveolar bone.					
37153993	4	172	theme	osteoporosis	622:633	arg1	model					613:617	the model	609:617	the model of osteoporosis with periodontitis	609:652	Except for the control group, the other two groups were ovariectomized and smeared with Porphyromonas gingivalis fluid to establish the model of osteoporosis with periodontitis.					
37153993	19	173	dep	CONCLUSIONS	3197:3207	arg1	induce					3238:3243	induce	3238:3243	induce	3238:3243	CONCLUSIONS Chitosan oligosaccharide can induce biochemical indexes of bone metabolism to become normal, alleviate the symptoms of periodontitis, this may be related to the inhibition of IKK/NF-κB pathway by chitosan oligosaccharide.					
37153993	5	174	from	rats	686:689	arg1	group					729:733	chitosan oligosaccharide treatment group	694:733	chitosan oligosaccharide treatment group	694:733	Four weeks after ligation, the rats in chitosan oligosaccharide treatment group were gavaged with 200 mg/kg chitosan oligosaccharide, and the other two groups were gavaged with equal volume of normal saline once a day for 90 days.					
37364812	0	0	from	analysis	28:35	arg1	exoskeleton					81:91	the exoskeleton	77:91	the exoskeleton of the horseshoe crab Limulus polyphemus	77:132	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.					
37364812	1	1	theme	chitin	145:150	arg1	biopolymer					152:161	chitin biopolymer	145:161	chitin biopolymer	145:161	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	2	2	theme	Limulus	517:523	arg1	polyphemus					525:534	the horseshoe crab Limulus polyphemus	498:534	the horseshoe crab Limulus polyphemus	498:534	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	1	3	theme	non-conventional	306:321	arg1	species					323:329	non-conventional species	306:329	non-conventional species as alternative sources of them compounds	306:370	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	4	4	theme	chemical	765:772	arg1	composition					774:784	chemical composition	765:784	chemical composition	765:784	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	4	5	attach	present	714:720	arg2	C					709:709	C	709:709	C	709:709	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	4	5	attach	present	714:720	arg1	proportion					737:746	the highest proportion	725:746	the highest proportion (~45 %)	725:754	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	4	5	attach	present	714:720	arg1	%					753:753	~45 %	749:753	~45 %	749:753	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	1	6	theme	species	323:329	arg1	study					297:301	the study	293:301	the study of non-conventional species as alternative sources of them compounds	293:370	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	7	7	theme	shaped	1304:1309	arg1	particles					1311:1319	irregularly shaped particles	1292:1319	irregularly shaped particles	1292:1319	SEM micrographs showed a porous matrix with infinite large number of irregularly shaped particles.					
37364812	7	8	theme	infinite	1267:1274	arg1	number					1282:1287	infinite large number	1267:1287	infinite large number of irregularly shaped particles	1267:1319	SEM micrographs showed a porous matrix with infinite large number of irregularly shaped particles.					
37364812	6	9	from	%	1145:1145	arg1	mass					1117:1120	the residual mass	1104:1120	the residual mass at 650 °C of around 30 % for both samples	1104:1162	TGA and DTGA profiles resulted very similar for both tagmata being the residual mass at 650 °C of around 30 % for both samples; these values were associated to the presence of minerals.					
37364812	6	9	from	%	1145:1145	arg1	tagmata					1090:1096	both tagmata	1085:1096	both tagmata	1085:1096	TGA and DTGA profiles resulted very similar for both tagmata being the residual mass at 650 °C of around 30 % for both samples; these values were associated to the presence of minerals.					
37364812	5	10	from	presence	981:988	arg1	exoskeleton					1016:1026	the exoskeleton	1012:1026	the exoskeleton studied	1012:1034	FTIR spectra of two tagmata presented a wide characteristic band of the chitin between 3600 and 3000 cm-1, confirming the presence of this biopolymer in the exoskeleton studied.					
37364812	7	11	theme	particles	1311:1319	arg1	number					1282:1287	infinite large number	1267:1287	infinite large number of irregularly shaped particles	1267:1319	SEM micrographs showed a porous matrix with infinite large number of irregularly shaped particles.					
37364812	0	12	theme	horseshoe	100:108	arg1	polyphemus					123:132	the horseshoe crab Limulus polyphemus	96:132	the horseshoe crab Limulus polyphemus	96:132	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.					
37364812	1	13	theme	alternative	334:344	arg1	sources					346:352	alternative sources	334:352	alternative sources of them compounds	334:370	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	5	14	attach	presence	981:988	arg1	exoskeleton					1016:1026	the exoskeleton	1012:1026	the exoskeleton studied	1012:1034	FTIR spectra of two tagmata presented a wide characteristic band of the chitin between 3600 and 3000 cm-1, confirming the presence of this biopolymer in the exoskeleton studied.					
37364812	5	14	attach	presence	981:988	arg2	biopolymer					998:1007	this biopolymer	993:1007	this biopolymer	993:1007	FTIR spectra of two tagmata presented a wide characteristic band of the chitin between 3600 and 3000 cm-1, confirming the presence of this biopolymer in the exoskeleton studied.					
37364812	2	15	theme	comparative	422:432	arg1	survey					450:455	a comparative physicochemical survey	420:455	a comparative physicochemical survey	420:455	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	0	16	from	quality	47:53	arg1	exoskeleton					81:91	the exoskeleton	77:91	the exoskeleton of the horseshoe crab Limulus polyphemus	77:132	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.					
37364812	7	17	with	matrix	1255:1260	arg1	number					1282:1287	infinite large number	1267:1287	infinite large number of irregularly shaped particles	1267:1319	SEM micrographs showed a porous matrix with infinite large number of irregularly shaped particles.					
37364812	5	18	theme	tagmata	879:885	arg1	spectra					864:870	FTIR spectra	859:870	FTIR spectra of two tagmata	859:885	FTIR spectra of two tagmata presented a wide characteristic band of the chitin between 3600 and 3000 cm-1, confirming the presence of this biopolymer in the exoskeleton studied.					
37364812	0	19	theme	physicochemical	12:26	arg1	analysis					28:35	Comparative physicochemical analysis	0:35	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.	0:133	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.					
37364812	2	20	from	survey	450:455	arg1	exoskeleton					483:493	the exoskeleton	479:493	the exoskeleton of the horseshoe crab Limulus polyphemus	479:534	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	7	21	theme	large	1276:1280	arg1	number					1282:1287	infinite large number	1267:1287	infinite large number of irregularly shaped particles	1267:1319	SEM micrographs showed a porous matrix with infinite large number of irregularly shaped particles.					
37364812	1	22	theme	much	176:179	arg1	attention					181:189	much attention	176:189	much attention	176:189	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	0	23	theme	Comparative	0:10	arg1	analysis					28:35	Comparative physicochemical analysis	0:35	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.	0:133	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.					
37364812	2	24	from	exoskeleton	483:493	arg1	survey					450:455	a comparative physicochemical survey	420:455	a comparative physicochemical survey	420:455	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	5	25	theme	wide	899:902	arg1	band					919:922	a wide characteristic band	897:922	a wide characteristic band of the chitin between 3600 and 3000 cm-1	897:963	FTIR spectra of two tagmata presented a wide characteristic band of the chitin between 3600 and 3000 cm-1, confirming the presence of this biopolymer in the exoskeleton studied.					
37364812	0	26	theme	Limulus	115:121	arg1	polyphemus					123:132	the horseshoe crab Limulus polyphemus	96:132	the horseshoe crab Limulus polyphemus	96:132	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.					
37364812	6	27	theme	residual	1108:1115	arg1	mass					1117:1120	the residual mass	1104:1120	the residual mass at 650 °C of around 30 % for both samples	1104:1162	TGA and DTGA profiles resulted very similar for both tagmata being the residual mass at 650 °C of around 30 % for both samples; these values were associated to the presence of minerals.					
37364812	6	27	theme	residual	1108:1115	arg1	tagmata					1090:1096	both tagmata	1085:1096	both tagmata	1085:1096	TGA and DTGA profiles resulted very similar for both tagmata being the residual mass at 650 °C of around 30 % for both samples; these values were associated to the presence of minerals.					
37364812	4	28	theme	CHNSO	680:684	arg1	analysis					686:693	The CHNSO analysis	676:693	The CHNSO analysis	676:693	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	6	29	theme	DTGA	1045:1048	arg1	profiles					1050:1057	DTGA profiles	1045:1057	DTGA profiles	1045:1057	TGA and DTGA profiles resulted very similar for both tagmata being the residual mass at 650 °C of around 30 % for both samples; these values were associated to the presence of minerals.					
37364812	8	30	theme	mineral	1405:1411	arg1	content					1413:1419	a high mineral content	1398:1419	a high mineral content	1398:1419	Results show that both tagmata are made up of chitin, and they seem to have a high mineral content.					
37364812	0	31	theme	crab	110:113	arg1	polyphemus					123:132	the horseshoe crab Limulus polyphemus	96:132	the horseshoe crab Limulus polyphemus	96:132	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.					
37364812	1	32	theme	them	357:360	arg1	sources					346:352	alternative sources	334:352	alternative sources of them compounds	334:370	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	5	33	theme	FTIR	859:862	arg1	spectra					864:870	FTIR spectra	859:870	FTIR spectra of two tagmata	859:885	FTIR spectra of two tagmata presented a wide characteristic band of the chitin between 3600 and 3000 cm-1, confirming the presence of this biopolymer in the exoskeleton studied.					
37364812	0	34	theme	quality	47:53	arg1	analysis					28:35	Comparative physicochemical analysis	0:35	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.	0:133	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.					
37364812	8	35	theme	high	1400:1403	arg1	content					1413:1419	a high mineral content	1398:1419	a high mineral content	1398:1419	Results show that both tagmata are made up of chitin, and they seem to have a high mineral content.					
37364812	2	36	theme	crab	512:515	arg1	polyphemus					525:534	the horseshoe crab Limulus polyphemus	498:534	the horseshoe crab Limulus polyphemus	498:534	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	7	37	theme	porous	1248:1253	arg1	matrix					1255:1260	a porous matrix	1246:1260	a porous matrix with infinite large number of irregularly shaped particles	1246:1319	SEM micrographs showed a porous matrix with infinite large number of irregularly shaped particles.					
37364812	1	38	theme	wide	196:199	arg1	application					223:233	biomedical application	212:233	biomedical application for this and its derivatives	212:262	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	1	38	theme	wide	196:199	arg1	variety					201:207	a wide variety	194:207	a wide variety of biomedical application for this and its derivatives	194:262	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	0	39	theme	chitin	40:45	arg1	quality					47:53	chitin quality	40:53	chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus	40:132	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.					
37364812	0	40	theme	polyphemus	123:132	arg1	exoskeleton					81:91	the exoskeleton	77:91	the exoskeleton of the horseshoe crab Limulus polyphemus	77:132	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.					
37364812	1	41	dep	them	357:360	arg1	compounds					362:370	compounds	362:370	them compounds	357:370	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	6	42	theme	minerals	1213:1220	arg1	presence					1201:1208	the presence	1197:1208	the presence of minerals	1197:1220	TGA and DTGA profiles resulted very similar for both tagmata being the residual mass at 650 °C of around 30 % for both samples; these values were associated to the presence of minerals.					
37364812	2	43	theme	physicochemical	434:448	arg1	survey					450:455	a comparative physicochemical survey	420:455	a comparative physicochemical survey	420:455	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	4	44	theme	significant	799:809	arg1	differences					811:821	significant differences	799:821	significant differences (P < 0.05) between the two tagmata	799:856	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	4	44	theme	significant	799:809	arg1	P < 0.05					824:831	P < 0.05	824:831	P < 0.05	824:831	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	2	45	from	tagmata	468:474	arg1	exoskeleton					483:493	the exoskeleton	479:493	the exoskeleton of the horseshoe crab Limulus polyphemus	479:534	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	5	46	theme	biopolymer	998:1007	arg1	presence					981:988	the presence	977:988	the presence of this biopolymer in the exoskeleton studied	977:1034	FTIR spectra of two tagmata presented a wide characteristic band of the chitin between 3600 and 3000 cm-1, confirming the presence of this biopolymer in the exoskeleton studied.					
37364812	2	47	theme	horseshoe	502:510	arg1	polyphemus					525:534	the horseshoe crab Limulus polyphemus	498:534	the horseshoe crab Limulus polyphemus	498:534	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	2	48	theme	polyphemus	525:534	arg1	exoskeleton					483:493	the exoskeleton	479:493	the exoskeleton of the horseshoe crab Limulus polyphemus	479:534	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	1	49	theme	particular	382:391	arg1	interest					393:400	particular interest	382:400	particular interest	382:400	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	5	50	theme	chitin	931:936	arg1	band					919:922	a wide characteristic band	897:922	a wide characteristic band of the chitin between 3600 and 3000 cm-1	897:963	FTIR spectra of two tagmata presented a wide characteristic band of the chitin between 3600 and 3000 cm-1, confirming the presence of this biopolymer in the exoskeleton studied.					
37364812	4	51	from	proportion	737:746	arg1	present					714:720	present	714:720	present	714:720	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	7	52	theme	SEM	1223:1225	arg1	micrographs					1227:1237	SEM micrographs	1223:1237	SEM micrographs	1223:1237	SEM micrographs showed a porous matrix with infinite large number of irregularly shaped particles.					
37364812	4	53	theme	highest	729:735	arg1	proportion					737:746	the highest proportion	725:746	the highest proportion (~45 %)	725:754	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	4	53	theme	highest	729:735	arg1	%					753:753	~45 %	749:753	~45 %	749:753	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	8	54	contain	have	1393:1396	arg1	they					1380:1383	they	1380:1383	they	1380:1383	Results show that both tagmata are made up of chitin, and they seem to have a high mineral content.					
37364812	8	54	contain	have	1393:1396	arg2	content					1413:1419	a high mineral content	1398:1419	a high mineral content	1398:1419	Results show that both tagmata are made up of chitin, and they seem to have a high mineral content.					
37364812	2	55	dep	tagmata	468:474	arg1	tagmata					468:474	the two tagmata	460:474	the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma	460:567	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	2	55	dep	tagmata	468:474	arg1	prosoma					541:547	the prosoma	537:547	the prosoma	537:547	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	2	55	dep	tagmata	468:474	arg1	opisthosoma					557:567	the opisthosoma	553:567	the opisthosoma	553:567	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	3	56	theme	CHNSO	630:634	arg1	analysis					636:643	CHNSO analysis	630:643	CHNSO analysis	630:643	The characterization included CHNSO analysis, FTIR, TGA, DSC, XRD, and SEM.					
37364812	1	57	theme	biomedical	212:221	arg1	application					223:233	biomedical application	212:233	biomedical application for this and its derivatives	212:262	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	0	58	theme	tagmata	66:72	arg1	quality					47:53	chitin quality	40:53	chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus	40:132	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.					
37364812	0	59	from	exoskeleton	81:91	arg1	analysis					28:35	Comparative physicochemical analysis	0:35	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.	0:133	Comparative physicochemical analysis of chitin quality of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus.					
37364812	4	60	located	present	714:720	arg2	C					709:709	C	709:709	C	709:709	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	4	60	located	present	714:720	arg1	proportion					737:746	the highest proportion	725:746	the highest proportion (~45 %)	725:754	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	4	60	located	present	714:720	arg1	%					753:753	~45 %	749:753	~45 %	749:753	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	1	61	theme	application	223:233	arg1	application					223:233	biomedical application	212:233	biomedical application for this and its derivatives	212:262	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	1	61	theme	application	223:233	arg1	variety					201:207	a wide variety	194:207	a wide variety of biomedical application for this and its derivatives	194:262	Recently, chitin biopolymer has received much attention as a wide variety of biomedical application for this and its derivatives have been reported, in fact, the study of non-conventional species as alternative sources of them compounds has taken particular interest.					
37364812	2	62	theme	tagmata	468:474	arg1	survey					450:455	a comparative physicochemical survey	420:455	a comparative physicochemical survey	420:455	Here, we present a comparative physicochemical survey of the two tagmata in the exoskeleton of the horseshoe crab Limulus polyphemus: the prosoma and the opisthosoma, collected in Yucatán, Mexico.					
37364812	5	63	theme	characteristic	904:917	arg1	band					919:922	a wide characteristic band	897:922	a wide characteristic band of the chitin between 3600 and 3000 cm-1	897:963	FTIR spectra of two tagmata presented a wide characteristic band of the chitin between 3600 and 3000 cm-1, confirming the presence of this biopolymer in the exoskeleton studied.					
37364812	4	64	from	present	714:720	arg1	proportion					737:746	the highest proportion	725:746	the highest proportion (~45 %)	725:754	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
37364812	4	64	from	present	714:720	arg1	%					753:753	~45 %	749:753	~45 %	749:753	The CHNSO analysis revealed that C is present in the highest proportion (~45 %) and that chemical composition did not show significant differences (P < 0.05) between the two tagmata.					
36327727	1	0	from	Hypothesis	62:71	arg1	suspensions					131:141	suspensions	131:141	suspensions	131:141	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is important both from the fundamental and advanced technology development perspective.					
36327727	10	1	theme	SAXS	1197:1200	arg1	analysis					1202:1209	SAXS analysis	1197:1209	SAXS analysis	1197:1209	SAXS analysis shows the short rods in suspension form an isotropic phase (randomly oriented) at lower concentration (0-4 wt%); as concentration is increased, the rods become systematically aligned in a nematic phase.					
36327727	8	2	theme	flux	962:965	arg1	SAXS					979:982	High flux synchrotron SAXS	957:982	High flux synchrotron SAXS	957:982	High flux synchrotron SAXS is applied to evaluate and compare the nanoscale self-assembly mechanisms of CNC of different lengths.					
36327727	11	3	dep	c-1	1515:1517	arg1	concentrations					1533:1546	the higher concentrations	1522:1546	the higher concentrations	1522:1546	The interrod distance d varies as c-0.33 at the lower concentration, which changes to c-0.5 and even c-1 at the higher concentrations.					
36327727	14	4	theme	CNC	1790:1792	arg1	rods					1794:1797	the short CNC rods	1780:1797	the short CNC rods	1780:1797	Suspensions made of the short CNC rods show long range order and large interrod distance compared to those formed by the long rods.					
36327727	10	5	from	rods	1227:1230	arg1	suspension					1235:1244	suspension	1235:1244	suspension form an isotropic phase (randomly oriented)	1235:1288	SAXS analysis shows the short rods in suspension form an isotropic phase (randomly oriented) at lower concentration (0-4 wt%); as concentration is increased, the rods become systematically aligned in a nematic phase.					
36327727	13	6	theme	isotropic	1723:1731	arg1	law					1739:1741	the isotropic power law	1719:1741	the isotropic power law slope of c-0.33	1719:1757	The interrod distance also follows the isotropic power law slope of c-0.33.					
36327727	14	7	theme	large	1825:1829	arg1	distance					1840:1847	large interrod distance	1825:1847	large interrod distance	1825:1847	Suspensions made of the short CNC rods show long range order and large interrod distance compared to those formed by the long rods.					
36327727	4	8	theme	Milli-Q	671:677	arg1	water					679:683	Milli-Q water	671:683	Milli-Q water	671:683	CNC-M is provided as a gel at a solid concentration of 10.3 wt% which was diluted in Milli-Q water.					
36327727	2	9	theme	repulsive	325:333	arg1	interactions					335:346	attractive and repulsive interactions	310:346	attractive and repulsive interactions which depends strongly on morphology, surface chemistry and concentrations	310:421	CNC of different lengths self-assemble differently in suspensions by balancing attractive and repulsive interactions which depends strongly on morphology, surface chemistry and concentrations.					
36327727	1	10	theme	fundamental	170:180	arg1	development					206:216	the fundamental and advanced technology development	166:216	the fundamental and advanced technology development perspective	166:228	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is important both from the fundamental and advanced technology development perspective.					
36327727	4	11	theme	10.3 wt	641:647	arg1	%					648:648	10.3 wt%	641:648	10.3 wt%	641:648	CNC-M is provided as a gel at a solid concentration of 10.3 wt% which was diluted in Milli-Q water.					
36327727	16	12	theme	rods	2036:2039	arg1	SA					2013:2014	SA	2013:2014	SA	2013:2014	A specific equilibrium between attractive and repulsive forces is required to maintain SA and ordering of the rods.					
36327727	16	12	theme	rods	2036:2039	arg1	ordering					2020:2027	ordering	2020:2027	ordering	2020:2027	A specific equilibrium between attractive and repulsive forces is required to maintain SA and ordering of the rods.					
36327727	2	13	theme	attractive	310:319	arg1	interactions					335:346	attractive and repulsive interactions	310:346	attractive and repulsive interactions which depends strongly on morphology, surface chemistry and concentrations	310:421	CNC of different lengths self-assemble differently in suspensions by balancing attractive and repulsive interactions which depends strongly on morphology, surface chemistry and concentrations.					
36327727	1	14	theme	advanced	186:193	arg1	development					206:216	the fundamental and advanced technology development	166:216	the fundamental and advanced technology development perspective	166:228	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is important both from the fundamental and advanced technology development perspective.					
36327727	7	15	theme	Polarised	846:854	arg1	microscopy					864:873	Polarised optical microscopy	846:873	Polarised optical microscopy	846:873	Polarised optical microscopy is performed to get microscale visualisation of the chiral nematic self-assembly.					
36327727	19	16	theme	SA	2409:2410	arg1	understanding					2388:2400	This fundamental understanding	2371:2400	This fundamental understanding of the SA of rods in suspensions	2371:2433	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	1	17	theme	development	206:216	arg1	perspective					218:228	the fundamental and advanced technology development perspective	166:228	the fundamental and advanced technology development perspective	166:228	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is important both from the fundamental and advanced technology development perspective.					
36327727	19	18	from	SA	2409:2410	arg1	suspensions					2423:2433	suspensions	2423:2433	suspensions	2423:2433	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	19	19	from	understanding	2388:2400	arg1	suspensions					2423:2433	suspensions	2423:2433	suspensions	2423:2433	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	14	20	theme	short	1784:1788	arg1	rods					1794:1797	the short CNC rods	1780:1797	the short CNC rods	1780:1797	Suspensions made of the short CNC rods show long range order and large interrod distance compared to those formed by the long rods.					
36327727	12	21	theme	concentration	1644:1656	arg1	range					1658:1662	the measured concentration range	1631:1662	the measured concentration range from 0.5 to 10 wt%	1631:1681	In contrast, the long rods in suspension remain in the isotropic phase throughout the measured concentration range from 0.5 to 10 wt%.					
36327727	17	22	theme	ordered	2205:2211	arg1	manner					2213:2218	an ordered manner	2202:2218	an ordered manner	2202:2218	DLVO calculations reveal that the long rods maintain van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner.					
36327727	1	23	dep	Hypothesis	62:71	arg1	self-assembly					77:89	The self-assembly	73:89	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions	62:141	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is important both from the fundamental and advanced technology development perspective.					
36327727	1	23	dep	Hypothesis	62:71	arg1	SA					92:93	SA	92:93	SA	92:93	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is important both from the fundamental and advanced technology development perspective.					
36327727	12	24	dep	10 wt	1676:1680	arg1	to					1673:1674	to	1673:1674	to	1673:1674	In contrast, the long rods in suspension remain in the isotropic phase throughout the measured concentration range from 0.5 to 10 wt%.					
36327727	11	25	theme	interrod	1418:1425	arg1	d					1436:1436	The interrod distance d	1414:1436	The interrod distance d	1414:1436	The interrod distance d varies as c-0.33 at the lower concentration, which changes to c-0.5 and even c-1 at the higher concentrations.					
36327727	19	26	theme	composites	2476:2485	arg1	engineering					2451:2461	the engineering	2447:2461	the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics	2447:2586	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	2	27	theme	different	238:246	arg1	lengths					248:254	different lengths	238:254	different lengths	238:254	CNC of different lengths self-assemble differently in suspensions by balancing attractive and repulsive interactions which depends strongly on morphology, surface chemistry and concentrations.					
36327727	19	28	theme	novel	2466:2470	arg1	composites					2476:2485	novel CNC composites	2466:2485	novel CNC composites	2466:2485	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	10	29	dep	suspension	1235:1244	arg1	form					1246:1249	form	1246:1249	form an isotropic phase	1246:1268	SAXS analysis shows the short rods in suspension form an isotropic phase (randomly oriented) at lower concentration (0-4 wt%); as concentration is increased, the rods become systematically aligned in a nematic phase.					
36327727	19	30	theme	properties	2505:2514	arg1	engineering					2451:2461	the engineering	2447:2461	the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics	2447:2586	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	8	31	theme	nanoscale	1023:1031	arg1	mechanisms					1047:1056	the nanoscale self-assembly mechanisms	1019:1056	the nanoscale self-assembly mechanisms of CNC of different lengths	1019:1084	High flux synchrotron SAXS is applied to evaluate and compare the nanoscale self-assembly mechanisms of CNC of different lengths.					
36327727	7	32	theme	nematic	934:940	arg1	self-assembly					942:954	the chiral nematic self-assembly	923:954	the chiral nematic self-assembly	923:954	Polarised optical microscopy is performed to get microscale visualisation of the chiral nematic self-assembly.					
36327727	19	33	theme	unique	2490:2495	arg1	properties					2505:2514	unique optical properties	2490:2514	unique optical properties which enables novel applications such as in sensors and bio-diagnostics	2490:2586	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	19	34	theme	novel	2530:2534	arg1	applications					2536:2547	novel applications	2530:2547	novel applications such as in sensors and bio-diagnostics	2530:2586	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	19	34	theme	novel	2530:2534	arg1	bio-diagnostics					2572:2586	bio-diagnostics	2572:2586	bio-diagnostics	2572:2586	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	19	34	theme	novel	2530:2534	arg1	sensors					2560:2566	sensors	2560:2566	sensors	2560:2566	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	4	35	from	concentration	624:636	arg1	gel					609:611	a gel	607:611	a gel at a solid concentration of 10.3 wt% which was diluted in Milli-Q water	607:683	CNC-M is provided as a gel at a solid concentration of 10.3 wt% which was diluted in Milli-Q water.					
36327727	4	35	from	concentration	624:636	arg1	CNC-M					586:590	CNC-M	586:590	CNC-M	586:590	CNC-M is provided as a gel at a solid concentration of 10.3 wt% which was diluted in Milli-Q water.					
36327727	17	36	theme	van	2095:2097	arg1	force					2119:2123	van der Waal attractive force	2095:2123	van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner	2095:2218	DLVO calculations reveal that the long rods maintain van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner.					
36327727	10	37	theme	isotropic	1254:1262	arg1	phase					1264:1268	an isotropic phase	1251:1268	an isotropic phase	1251:1268	SAXS analysis shows the short rods in suspension form an isotropic phase (randomly oriented) at lower concentration (0-4 wt%); as concentration is increased, the rods become systematically aligned in a nematic phase.					
36327727	6	38	used	used	809:812	arg2	TEM					801:803	TEM	801:803	TEM	801:803	TEM was used to determine morphology of CNC.					
36327727	8	39	theme	CNC	1061:1063	arg1	mechanisms					1047:1056	the nanoscale self-assembly mechanisms	1019:1056	the nanoscale self-assembly mechanisms of CNC of different lengths	1019:1084	High flux synchrotron SAXS is applied to evaluate and compare the nanoscale self-assembly mechanisms of CNC of different lengths.					
36327727	12	40	theme	long	1566:1569	arg1	rods					1571:1574	the long rods	1562:1574	the long rods in suspension	1562:1588	In contrast, the long rods in suspension remain in the isotropic phase throughout the measured concentration range from 0.5 to 10 wt%.					
36327727	15	41	theme	SAXS	1912:1915	arg1	results					1917:1923	the SAXS results	1908:1923	the SAXS results	1908:1923	POM agrees with the SAXS results.					
36327727	17	42	theme	Waal	2103:2106	arg1	force					2119:2123	van der Waal attractive force	2095:2123	van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner	2095:2218	DLVO calculations reveal that the long rods maintain van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner.					
36327727	11	43	theme	distance	1427:1434	arg1	d					1436:1436	The interrod distance d	1414:1436	The interrod distance d	1414:1436	The interrod distance d varies as c-0.33 at the lower concentration, which changes to c-0.5 and even c-1 at the higher concentrations.					
36327727	10	44	theme	short	1221:1225	arg1	rods					1227:1230	the short rods	1217:1230	the short rods in suspension form an isotropic phase (randomly oriented)	1217:1288	SAXS analysis shows the short rods in suspension form an isotropic phase (randomly oriented) at lower concentration (0-4 wt%); as concentration is increased, the rods become systematically aligned in a nematic phase.					
36327727	0	45	theme	cellulose	17:25	arg1	nanocrystals					27:38	cellulose nanocrystals	17:38	cellulose nanocrystals of different lengths	17:59	Self-assembly of cellulose nanocrystals of different lengths.					
36327727	8	46	theme	different	1068:1076	arg1	lengths					1078:1084	different lengths	1068:1084	different lengths	1068:1084	High flux synchrotron SAXS is applied to evaluate and compare the nanoscale self-assembly mechanisms of CNC of different lengths.					
36327727	1	47	theme	cellulose	99:107	arg1	CNC					123:125	CNC	123:125	CNC	123:125	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is important both from the fundamental and advanced technology development perspective.					
36327727	1	47	theme	cellulose	99:107	arg1	nanocrystals					109:120	cellulose nanocrystals	99:120	cellulose nanocrystals (CNC)	99:126	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is important both from the fundamental and advanced technology development perspective.					
36327727	3	48	theme	commercial	450:459	arg1	CNC-M					474:478	CNC-M	474:478	CNC-M	474:478	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	48	theme	commercial	450:459	arg1	samples					465:471	Two different commercial CNC samples	436:471	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths	424:510	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	48	theme	commercial	450:459	arg1	CNC-C					484:488	CNC-C	484:488	CNC-C	484:488	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	10	49	theme	oriented	1280:1287	arg1	suspension					1235:1244	suspension	1235:1244	suspension form an isotropic phase (randomly oriented)	1235:1288	SAXS analysis shows the short rods in suspension form an isotropic phase (randomly oriented) at lower concentration (0-4 wt%); as concentration is increased, the rods become systematically aligned in a nematic phase.					
36327727	13	50	dep	law	1739:1741	arg1	slope					1743:1747	slope	1743:1747	the isotropic power law slope of c-0.33	1719:1757	The interrod distance also follows the isotropic power law slope of c-0.33.					
36327727	19	51	from	suspensions	2423:2433	arg1	understanding					2388:2400	This fundamental understanding	2371:2400	This fundamental understanding of the SA of rods in suspensions	2371:2433	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	16	52	theme	attractive	1957:1966	arg1	forces					1982:1987	attractive and repulsive forces	1957:1987	attractive and repulsive forces	1957:1987	A specific equilibrium between attractive and repulsive forces is required to maintain SA and ordering of the rods.					
36327727	9	53	theme	types	1120:1124	arg1	SA					1100:1101	The SA	1096:1101	The SA of two different types of CNC rods of similar diameter	1096:1156	Findings The SA of two different types of CNC rods of similar diameter but different lengths is investigated.					
36327727	0	54	theme	lengths	53:59	arg1	nanocrystals					27:38	cellulose nanocrystals	17:38	cellulose nanocrystals of different lengths	17:59	Self-assembly of cellulose nanocrystals of different lengths.					
36327727	14	55	theme	long	1881:1884	arg1	rods					1886:1889	the long rods	1877:1889	the long rods	1877:1889	Suspensions made of the short CNC rods show long range order and large interrod distance compared to those formed by the long rods.					
36327727	18	56	theme	short	2238:2242	arg1	rods					2244:2247	the short rods	2234:2247	the short rods	2234:2247	However, for the short rods, the weaker attractive interactions are well compensated by the repulsive force which aligns rods in an ordered assembly.					
36327727	16	57	theme	repulsive	1972:1980	arg1	forces					1982:1987	attractive and repulsive forces	1957:1987	attractive and repulsive forces	1957:1987	A specific equilibrium between attractive and repulsive forces is required to maintain SA and ordering of the rods.					
36327727	17	58	theme	DLVO	2042:2045	arg1	calculations					2047:2058	DLVO calculations	2042:2058	DLVO calculations	2042:2058	DLVO calculations reveal that the long rods maintain van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner.					
36327727	9	59	theme	CNC	1129:1131	arg1	rods					1133:1136	CNC rods	1129:1136	CNC rods of similar diameter	1129:1156	Findings The SA of two different types of CNC rods of similar diameter but different lengths is investigated.					
36327727	3	60	theme	lengths	504:510	arg1	CNC-M					474:478	CNC-M	474:478	CNC-M	474:478	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	60	theme	lengths	504:510	arg1	samples					465:471	Two different commercial CNC samples	436:471	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths	424:510	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	60	theme	lengths	504:510	arg1	CNC-C					484:488	CNC-C	484:488	CNC-C	484:488	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	7	61	theme	microscale	895:904	arg1	visualisation					906:918	microscale visualisation	895:918	microscale visualisation of the chiral nematic self-assembly	895:954	Polarised optical microscopy is performed to get microscale visualisation of the chiral nematic self-assembly.					
36327727	11	62	theme	higher	1526:1531	arg1	concentrations					1533:1546	the higher concentrations	1522:1546	the higher concentrations	1522:1546	The interrod distance d varies as c-0.33 at the lower concentration, which changes to c-0.5 and even c-1 at the higher concentrations.					
36327727	13	63	theme	power	1733:1737	arg1	law					1739:1741	the isotropic power law	1719:1741	the isotropic power law slope of c-0.33	1719:1757	The interrod distance also follows the isotropic power law slope of c-0.33.					
36327727	18	64	theme	repulsive	2313:2321	arg1	force					2323:2327	the repulsive force	2309:2327	the repulsive force which aligns rods in an ordered assembly	2309:2368	However, for the short rods, the weaker attractive interactions are well compensated by the repulsive force which aligns rods in an ordered assembly.					
36327727	8	65	theme	High	957:960	arg1	SAXS					979:982	High flux synchrotron SAXS	957:982	High flux synchrotron SAXS	957:982	High flux synchrotron SAXS is applied to evaluate and compare the nanoscale self-assembly mechanisms of CNC of different lengths.					
36327727	4	66	theme	solid	618:622	arg1	concentration					624:636	a solid concentration	616:636	a solid concentration of 10.3 wt% which was diluted in Milli-Q water	616:683	CNC-M is provided as a gel at a solid concentration of 10.3 wt% which was diluted in Milli-Q water.					
36327727	9	67	theme	diameter	1149:1156	arg1	rods					1133:1136	CNC rods	1129:1136	CNC rods of similar diameter	1129:1156	Findings The SA of two different types of CNC rods of similar diameter but different lengths is investigated.					
36327727	3	68	theme	Milli-Q	530:536	arg1	water					538:542	Milli-Q water	530:542	Milli-Q water	530:542	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	18	69	theme	ordered	2353:2359	arg1	assembly					2361:2368	an ordered assembly	2350:2368	an ordered assembly	2350:2368	However, for the short rods, the weaker attractive interactions are well compensated by the repulsive force which aligns rods in an ordered assembly.					
36327727	8	70	theme	synchrotron	967:977	arg1	SAXS					979:982	High flux synchrotron SAXS	957:982	High flux synchrotron SAXS	957:982	High flux synchrotron SAXS is applied to evaluate and compare the nanoscale self-assembly mechanisms of CNC of different lengths.					
36327727	9	71	theme	different	1162:1170	arg1	lengths					1172:1178	different lengths	1162:1178	different lengths	1162:1178	Findings The SA of two different types of CNC rods of similar diameter but different lengths is investigated.					
36327727	7	72	theme	optical	856:862	arg1	microscopy					864:873	Polarised optical microscopy	846:873	Polarised optical microscopy	846:873	Polarised optical microscopy is performed to get microscale visualisation of the chiral nematic self-assembly.					
36327727	14	73	theme	interrod	1831:1838	arg1	distance					1840:1847	large interrod distance	1825:1847	large interrod distance	1825:1847	Suspensions made of the short CNC rods show long range order and large interrod distance compared to those formed by the long rods.					
36327727	18	74	theme	weaker	2254:2259	arg1	interactions					2272:2283	the weaker attractive interactions	2250:2283	the weaker attractive interactions	2250:2283	However, for the short rods, the weaker attractive interactions are well compensated by the repulsive force which aligns rods in an ordered assembly.					
36327727	12	75	from	rods	1571:1574	arg1	suspension					1579:1588	suspension	1579:1588	suspension	1579:1588	In contrast, the long rods in suspension remain in the isotropic phase throughout the measured concentration range from 0.5 to 10 wt%.					
36327727	13	76	theme	interrod	1688:1695	arg1	distance					1697:1704	The interrod distance	1684:1704	The interrod distance	1684:1704	The interrod distance also follows the isotropic power law slope of c-0.33.					
36327727	14	77	theme	range	1809:1813	arg1	order					1815:1819	long range order	1804:1819	long range order	1804:1819	Suspensions made of the short CNC rods show long range order and large interrod distance compared to those formed by the long rods.					
36327727	1	78	theme	technology	195:204	arg1	development					206:216	the fundamental and advanced technology development	166:216	the fundamental and advanced technology development perspective	166:228	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is important both from the fundamental and advanced technology development perspective.					
36327727	1	79	dep	perspective	218:228	arg1	both					156:159	both	156:159	both	156:159	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is important both from the fundamental and advanced technology development perspective.					
36327727	4	80	theme	%	648:648	arg1	concentration					624:636	a solid concentration	616:636	a solid concentration of 10.3 wt% which was diluted in Milli-Q water	616:683	CNC-M is provided as a gel at a solid concentration of 10.3 wt% which was diluted in Milli-Q water.					
36327727	19	81	theme	rods	2415:2418	arg1	SA					2409:2410	the SA	2405:2410	the SA of rods in suspensions	2405:2433	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	1	82	theme	nanocrystals	109:120	arg1	Hypothesis					62:71	Hypothesis	62:71	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions	62:141	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is important both from the fundamental and advanced technology development perspective.					
36327727	1	83	from	perspective	218:228	arg1	important					146:154	important	146:154	important	146:154	Hypothesis The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is important both from the fundamental and advanced technology development perspective.					
36327727	12	84	theme	measured	1635:1642	arg1	range					1658:1662	the measured concentration range	1631:1662	the measured concentration range from 0.5 to 10 wt%	1631:1681	In contrast, the long rods in suspension remain in the isotropic phase throughout the measured concentration range from 0.5 to 10 wt%.					
36327727	14	85	theme	long	1804:1807	arg1	order					1815:1819	long range order	1804:1819	long range order	1804:1819	Suspensions made of the short CNC rods show long range order and large interrod distance compared to those formed by the long rods.					
36327727	5	86	theme	Milli-Q	735:741	arg1	water					743:747	Milli-Q water	735:747	Milli-Q water with a mixer to achieve the desired concentrations	735:798	CNC-C is sold as a powder which was dispersed in Milli-Q water with a mixer to achieve the desired concentrations.					
36327727	2	87	theme	lengths	248:254	arg1	CNC					231:233	CNC	231:233	CNC of different lengths	231:254	CNC of different lengths self-assemble differently in suspensions by balancing attractive and repulsive interactions which depends strongly on morphology, surface chemistry and concentrations.					
36327727	5	88	with	water	743:747	arg1	mixer					756:760	a mixer	754:760	a mixer to achieve the desired concentrations	754:798	CNC-C is sold as a powder which was dispersed in Milli-Q water with a mixer to achieve the desired concentrations.					
36327727	11	89	theme	lower	1462:1466	arg1	concentration					1468:1480	the lower concentration	1458:1480	the lower concentration	1458:1480	The interrod distance d varies as c-0.33 at the lower concentration, which changes to c-0.5 and even c-1 at the higher concentrations.					
36327727	12	90	theme	isotropic	1604:1612	arg1	phase					1614:1618	the isotropic phase	1600:1618	the isotropic phase	1600:1618	In contrast, the long rods in suspension remain in the isotropic phase throughout the measured concentration range from 0.5 to 10 wt%.					
36327727	10	91	theme	nematic	1399:1405	arg1	phase					1407:1411	a nematic phase	1397:1411	a nematic phase	1397:1411	SAXS analysis shows the short rods in suspension form an isotropic phase (randomly oriented) at lower concentration (0-4 wt%); as concentration is increased, the rods become systematically aligned in a nematic phase.					
36327727	19	92	theme	CNC	2472:2474	arg1	composites					2476:2485	novel CNC composites	2466:2485	novel CNC composites	2466:2485	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	8	93	theme	self-assembly	1033:1045	arg1	mechanisms					1047:1056	the nanoscale self-assembly mechanisms	1019:1056	the nanoscale self-assembly mechanisms of CNC of different lengths	1019:1084	High flux synchrotron SAXS is applied to evaluate and compare the nanoscale self-assembly mechanisms of CNC of different lengths.					
36327727	17	94	theme	electrostatic	2145:2157	arg1	repulsion					2159:2167	the electrostatic repulsion	2141:2167	the electrostatic repulsion	2141:2167	DLVO calculations reveal that the long rods maintain van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner.					
36327727	3	95	dep	Experiments	424:434	arg1	CNC-M					474:478	CNC-M	474:478	CNC-M	474:478	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	95	dep	Experiments	424:434	arg1	samples					465:471	Two different commercial CNC samples	436:471	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths	424:510	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	95	dep	Experiments	424:434	arg1	CNC-C					484:488	CNC-C	484:488	CNC-C	484:488	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	17	96	theme	long	2076:2079	arg1	rods					2081:2084	the long rods	2072:2084	the long rods	2072:2084	DLVO calculations reveal that the long rods maintain van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner.					
36327727	7	97	theme	self-assembly	942:954	arg1	visualisation					906:918	microscale visualisation	895:918	microscale visualisation of the chiral nematic self-assembly	895:954	Polarised optical microscopy is performed to get microscale visualisation of the chiral nematic self-assembly.					
36327727	5	98	theme	desired	777:783	arg1	concentrations					785:798	the desired concentrations	773:798	the desired concentrations	773:798	CNC-C is sold as a powder which was dispersed in Milli-Q water with a mixer to achieve the desired concentrations.					
36327727	19	99	theme	optical	2497:2503	arg1	properties					2505:2514	unique optical properties	2490:2514	unique optical properties which enables novel applications such as in sensors and bio-diagnostics	2490:2586	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	7	100	theme	chiral	927:932	arg1	self-assembly					942:954	the chiral nematic self-assembly	923:954	the chiral nematic self-assembly	923:954	Polarised optical microscopy is performed to get microscale visualisation of the chiral nematic self-assembly.					
36327727	17	101	theme	der	2099:2101	arg1	force					2119:2123	van der Waal attractive force	2095:2123	van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner	2095:2218	DLVO calculations reveal that the long rods maintain van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner.					
36327727	10	102	theme	lower	1293:1297	arg1	concentration					1299:1311	lower concentration	1293:1311	lower concentration (0-4 wt%)	1293:1321	SAXS analysis shows the short rods in suspension form an isotropic phase (randomly oriented) at lower concentration (0-4 wt%); as concentration is increased, the rods become systematically aligned in a nematic phase.					
36327727	10	102	theme	lower	1293:1297	arg1	%					1320:1320	0-4 wt%	1314:1320	0-4 wt%	1314:1320	SAXS analysis shows the short rods in suspension form an isotropic phase (randomly oriented) at lower concentration (0-4 wt%); as concentration is increased, the rods become systematically aligned in a nematic phase.					
36327727	17	103	theme	rods	2184:2187	arg1	alignment					2189:2197	rods alignment	2184:2197	rods alignment	2184:2197	DLVO calculations reveal that the long rods maintain van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner.					
36327727	0	104	theme	nanocrystals	27:38	arg1	Self-assembly					0:12	Self-assembly	0:12	Self-assembly of cellulose nanocrystals of different lengths.	0:60	Self-assembly of cellulose nanocrystals of different lengths.					
36327727	8	105	theme	lengths	1078:1084	arg1	CNC					1061:1063	CNC	1061:1063	CNC of different lengths	1061:1084	High flux synchrotron SAXS is applied to evaluate and compare the nanoscale self-assembly mechanisms of CNC of different lengths.					
36327727	12	106	from	%	1681:1681	arg1	range					1658:1662	the measured concentration range	1631:1662	the measured concentration range from 0.5 to 10 wt%	1631:1681	In contrast, the long rods in suspension remain in the isotropic phase throughout the measured concentration range from 0.5 to 10 wt%.					
36327727	17	107	theme	attractive	2108:2117	arg1	force					2119:2123	van der Waal attractive force	2095:2123	van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner	2095:2218	DLVO calculations reveal that the long rods maintain van der Waal attractive force dominating over the electrostatic repulsion, which hinders rods alignment in an ordered manner.					
36327727	3	108	theme	different	494:502	arg1	lengths					504:510	different lengths	494:510	different lengths	494:510	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	0	109	theme	different	43:51	arg1	lengths					53:59	different lengths	43:59	different lengths	43:59	Self-assembly of cellulose nanocrystals of different lengths.					
36327727	6	110	theme	CNC	841:843	arg1	morphology					827:836	morphology	827:836	morphology of CNC	827:843	TEM was used to determine morphology of CNC.					
36327727	3	111	dep	samples	465:471	arg1	CNC-M					474:478	CNC-M	474:478	CNC-M	474:478	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	111	dep	samples	465:471	arg1	samples					465:471	Two different commercial CNC samples	436:471	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths	424:510	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	111	dep	samples	465:471	arg1	CNC-C					484:488	CNC-C	484:488	CNC-C	484:488	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	112	theme	CNC	461:463	arg1	CNC-M					474:478	CNC-M	474:478	CNC-M	474:478	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	112	theme	CNC	461:463	arg1	samples					465:471	Two different commercial CNC samples	436:471	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths	424:510	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	112	theme	CNC	461:463	arg1	CNC-C					484:488	CNC-C	484:488	CNC-C	484:488	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	16	113	theme	specific	1928:1935	arg1	equilibrium					1937:1947	A specific equilibrium	1926:1947	A specific equilibrium between attractive and repulsive forces	1926:1987	A specific equilibrium between attractive and repulsive forces is required to maintain SA and ordering of the rods.					
36327727	9	114	theme	different	1110:1118	arg1	types					1120:1124	two different types	1106:1124	two different types of CNC rods of similar diameter	1106:1156	Findings The SA of two different types of CNC rods of similar diameter but different lengths is investigated.					
36327727	13	115	theme	c-0.33	1752:1757	arg1	law					1739:1741	the isotropic power law	1719:1741	the isotropic power law slope of c-0.33	1719:1757	The interrod distance also follows the isotropic power law slope of c-0.33.					
36327727	3	116	theme	different	440:448	arg1	CNC-M					474:478	CNC-M	474:478	CNC-M	474:478	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	116	theme	different	440:448	arg1	samples					465:471	Two different commercial CNC samples	436:471	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths	424:510	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	116	theme	different	440:448	arg1	CNC-C					484:488	CNC-C	484:488	CNC-C	484:488	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	18	117	theme	attractive	2261:2270	arg1	interactions					2272:2283	the weaker attractive interactions	2250:2283	the weaker attractive interactions	2250:2283	However, for the short rods, the weaker attractive interactions are well compensated by the repulsive force which aligns rods in an ordered assembly.					
36327727	9	118	theme	rods	1133:1136	arg1	types					1120:1124	two different types	1106:1124	two different types of CNC rods of similar diameter	1106:1156	Findings The SA of two different types of CNC rods of similar diameter but different lengths is investigated.					
36327727	2	119	theme	surface	386:392	arg1	chemistry					394:402	surface chemistry	386:402	surface chemistry	386:402	CNC of different lengths self-assemble differently in suspensions by balancing attractive and repulsive interactions which depends strongly on morphology, surface chemistry and concentrations.					
36327727	19	120	theme	fundamental	2376:2386	arg1	understanding					2388:2400	This fundamental understanding	2371:2400	This fundamental understanding of the SA of rods in suspensions	2371:2433	This fundamental understanding of the SA of rods in suspensions facilitates the engineering of novel CNC composites of unique optical properties which enables novel applications such as in sensors and bio-diagnostics.					
36327727	9	121	theme	similar	1141:1147	arg1	diameter					1149:1156	similar diameter	1141:1156	similar diameter	1141:1156	Findings The SA of two different types of CNC rods of similar diameter but different lengths is investigated.					
36327727	3	122	theme	different	547:555	arg1	concentrations					557:570	different concentrations	547:570	different concentrations (0.5-10 wt%)	547:583	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36327727	3	122	theme	different	547:555	arg1	%					582:582	0.5-10 wt%	573:582	0.5-10 wt%	573:582	Experiments Two different commercial CNC samples (CNC-M and CNC-C) of different lengths were dispersed in Milli-Q water at different concentrations (0.5-10 wt%).					
36040455	6	0	theme	chitin/ZnO	1043:1052	arg1	hydrogels					1064:1072	the chitin/ZnO composite hydrogels	1039:1072	the chitin/ZnO composite hydrogels	1039:1072	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	2	1	theme	infected	374:381	arg1	material					417:424	an infected full-thickness skin wound-healing material	371:424	an infected full-thickness skin wound-healing material	371:424	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	2	1	theme	infected	374:381	arg1	hydrogels					339:347	multifunctional chitin/ZnO composite hydrogels	302:347	multifunctional chitin/ZnO composite hydrogels	302:347	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	4	2	theme	ZnO	640:642	arg1	particles					644:652	The regenerated nanoscale ZnO particles	614:652	The regenerated nanoscale ZnO particles	614:652	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	4	2	theme	ZnO	640:642	arg1	embedded					698:705	embedded	698:705	embedded	698:705	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	5	3	theme	chitin/ZnO	844:853	arg1	hydrogels					865:873	the chitin/ZnO composite hydrogels	840:873	the chitin/ZnO composite hydrogels	840:873	The decoration of ZnO endows the chitin/ZnO composite hydrogels with excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with acceptable biocompatibility.					
36040455	3	4	theme	aqueous	548:554	arg1	crosslinking					583:594	crosslinking	583:594	crosslinking	583:594	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	3	4	theme	aqueous	548:554	arg1	solutions					572:580	aqueous alkaline chitin solutions	548:580	aqueous alkaline chitin solutions	548:580	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	3	4	theme	aqueous	548:554	arg1	regeneration					600:611	regeneration	600:611	regeneration	600:611	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	4	5	theme	nanoscale	630:638	arg1	particles					644:652	The regenerated nanoscale ZnO particles	614:652	The regenerated nanoscale ZnO particles	614:652	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	4	5	theme	nanoscale	630:638	arg1	embedded					698:705	embedded	698:705	embedded	698:705	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	7	6	theme	chitin/ZnO	1317:1326	arg1	dressings					1384:1392	competitive skin wound dressings	1361:1392	competitive skin wound dressings for the prevention and control of infections	1361:1437	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	7	6	theme	chitin/ZnO	1317:1326	arg1	hydrogels					1338:1346	the chitin/ZnO composite hydrogels	1313:1346	the chitin/ZnO composite hydrogels	1313:1346	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	3	7	theme	alkaline	556:563	arg1	crosslinking					583:594	crosslinking	583:594	crosslinking	583:594	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	3	7	theme	alkaline	556:563	arg1	solutions					572:580	aqueous alkaline chitin solutions	548:580	aqueous alkaline chitin solutions	548:580	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	3	7	theme	alkaline	556:563	arg1	regeneration					600:611	regeneration	600:611	regeneration	600:611	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	6	8	theme	granulation	1262:1272	arg1	tissue					1274:1279	granulation tissue	1262:1279	granulation tissue	1262:1279	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	7	9	theme	composite	1328:1336	arg1	dressings					1384:1392	competitive skin wound dressings	1361:1392	competitive skin wound dressings for the prevention and control of infections	1361:1437	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	7	9	theme	composite	1328:1336	arg1	hydrogels					1338:1346	the chitin/ZnO composite hydrogels	1313:1346	the chitin/ZnO composite hydrogels	1313:1346	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	7	10	theme	skin	1373:1376	arg1	dressings					1384:1392	competitive skin wound dressings	1361:1392	competitive skin wound dressings for the prevention and control of infections	1361:1437	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	7	10	theme	skin	1373:1376	arg1	hydrogels					1338:1346	the chitin/ZnO composite hydrogels	1313:1346	the chitin/ZnO composite hydrogels	1313:1346	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	4	11	theme	hydrogen	763:770	arg1	bonding					772:778	hydrogen bonding	763:778	hydrogen bonding	763:778	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	3	12	theme	commercial	520:529	arg1	powders					535:541	commercial ZnO powders	520:541	commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration	520:611	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	6	13	theme	fibroblast	1168:1177	arg1	proliferation					1179:1191	more fibroblast proliferation	1163:1191	more fibroblast proliferation	1163:1191	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	7	14	dep	prevention	1402:1411	arg1	the					1398:1400	the	1398:1400	the	1398:1400	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	6	15	theme	more	1194:1197	arg1	deposition					1208:1217	more collagen deposition	1194:1217	more collagen deposition	1194:1217	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	3	16	theme	one-pot	468:474	arg1	strategy					476:483	a facile one-pot strategy	459:483	a facile one-pot strategy	459:483	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	6	17	theme	accelerated	1094:1104	arg1	performance					1146:1156	an outstanding accelerated infectious full-thickness wound-healing performance	1079:1156	an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue	1079:1279	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	4	18	from	matrix	721:726	arg1	particles					644:652	The regenerated nanoscale ZnO particles	614:652	The regenerated nanoscale ZnO particles	614:652	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	4	18	from	matrix	721:726	arg1	embedded					698:705	embedded	698:705	embedded	698:705	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	1	19	theme	appropriate	164:174	arg1	dressings					176:184	thus appropriate dressings	159:184	thus appropriate dressings with both antibacterial ability and healing ability for bacteria-associated wounds	159:267	When ordinary wounds are infected, the skin's self-healing capacity declines; thus appropriate dressings with both antibacterial ability and healing ability for bacteria-associated wounds are indispensable.					
36040455	5	20	theme	antibacterial	890:902	arg1	activity					904:911	excellent antibacterial activity	880:911	excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus)	880:984	The decoration of ZnO endows the chitin/ZnO composite hydrogels with excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with acceptable biocompatibility.					
36040455	1	21	theme	ordinary	86:93	arg1	wounds					95:100	ordinary wounds	86:100	ordinary wounds	86:100	When ordinary wounds are infected, the skin's self-healing capacity declines; thus appropriate dressings with both antibacterial ability and healing ability for bacteria-associated wounds are indispensable.					
36040455	4	22	theme	tight	733:737	arg1	interactions					739:750	tight interactions	733:750	tight interactions	733:750	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	4	22	theme	tight	733:737	arg1	bonding					772:778	hydrogen bonding	763:778	hydrogen bonding	763:778	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	4	22	theme	tight	733:737	arg1	interactions					797:808	coordination interactions	784:808	coordination interactions	784:808	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	0	23	theme	Facile	0:5	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of chitin/ZnO composite	0:41	Facile fabrication of chitin/ZnO composite hydrogels for infected wound healing.					
36040455	4	24	theme	coordination	784:795	arg1	interactions					797:808	coordination interactions	784:808	coordination interactions	784:808	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	2	25	theme	composite	329:337	arg1	material					417:424	an infected full-thickness skin wound-healing material	371:424	an infected full-thickness skin wound-healing material	371:424	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	2	25	theme	composite	329:337	arg1	hydrogels					339:347	multifunctional chitin/ZnO composite hydrogels	302:347	multifunctional chitin/ZnO composite hydrogels	302:347	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	2	26	theme	wound-healing	403:415	arg1	material					417:424	an infected full-thickness skin wound-healing material	371:424	an infected full-thickness skin wound-healing material	371:424	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	2	26	theme	wound-healing	403:415	arg1	hydrogels					339:347	multifunctional chitin/ZnO composite hydrogels	302:347	multifunctional chitin/ZnO composite hydrogels	302:347	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	0	27	theme	chitin/ZnO	22:31	arg1	composite					33:41	chitin/ZnO composite	22:41	chitin/ZnO composite	22:41	Facile fabrication of chitin/ZnO composite hydrogels for infected wound healing.					
36040455	6	28	theme	collagen	1199:1206	arg1	deposition					1208:1217	more collagen deposition	1194:1217	more collagen deposition	1194:1217	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	2	29	theme	chitin/ZnO	318:327	arg1	material					417:424	an infected full-thickness skin wound-healing material	371:424	an infected full-thickness skin wound-healing material	371:424	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	2	29	theme	chitin/ZnO	318:327	arg1	hydrogels					339:347	multifunctional chitin/ZnO composite hydrogels	302:347	multifunctional chitin/ZnO composite hydrogels	302:347	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	3	30	theme	sequential	497:506	arg1	addition					508:515	the sequential addition	493:515	the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration	493:611	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	4	31	theme	regenerated	618:628	arg1	particles					644:652	The regenerated nanoscale ZnO particles	614:652	The regenerated nanoscale ZnO particles	614:652	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	4	31	theme	regenerated	618:628	arg1	embedded					698:705	embedded	698:705	embedded	698:705	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	4	32	theme	microscale	669:678	arg1	particles					680:688	microscale particles	669:688	microscale particles	669:688	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	2	33	theme	multifunctional	302:316	arg1	material					417:424	an infected full-thickness skin wound-healing material	371:424	an infected full-thickness skin wound-healing material	371:424	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	2	33	theme	multifunctional	302:316	arg1	hydrogels					339:347	multifunctional chitin/ZnO composite hydrogels	302:347	multifunctional chitin/ZnO composite hydrogels	302:347	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	4	34	from	embedded	698:705	arg1	matrix					721:726	the chitin matrix	710:726	the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions	710:808	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	1	35	theme	antibacterial	196:208	arg1	ability					210:216	antibacterial ability	196:216	antibacterial ability	196:216	When ordinary wounds are infected, the skin's self-healing capacity declines; thus appropriate dressings with both antibacterial ability and healing ability for bacteria-associated wounds are indispensable.					
36040455	5	36	theme	composite	855:863	arg1	hydrogels					865:873	the chitin/ZnO composite hydrogels	840:873	the chitin/ZnO composite hydrogels	840:873	The decoration of ZnO endows the chitin/ZnO composite hydrogels with excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with acceptable biocompatibility.					
36040455	6	37	theme	epithelium	1247:1256	arg1	proliferation					1179:1191	more fibroblast proliferation	1163:1191	more fibroblast proliferation	1163:1191	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	6	37	theme	epithelium	1247:1256	arg1	neogenesis					1229:1238	more neogenesis	1224:1238	more neogenesis	1224:1238	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	6	37	theme	epithelium	1247:1256	arg1	deposition					1208:1217	more collagen deposition	1194:1217	more collagen deposition	1194:1217	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	7	38	theme	wound	1378:1382	arg1	dressings					1384:1392	competitive skin wound dressings	1361:1392	competitive skin wound dressings for the prevention and control of infections	1361:1437	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	7	38	theme	wound	1378:1382	arg1	hydrogels					1338:1346	the chitin/ZnO composite hydrogels	1313:1346	the chitin/ZnO composite hydrogels	1313:1346	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	3	39	theme	facile	461:466	arg1	strategy					476:483	a facile one-pot strategy	459:483	a facile one-pot strategy	459:483	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	6	40	theme	more	1163:1166	arg1	proliferation					1179:1191	more fibroblast proliferation	1163:1191	more fibroblast proliferation	1163:1191	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	0	41	theme	composite	33:41	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of chitin/ZnO composite	0:41	Facile fabrication of chitin/ZnO composite hydrogels for infected wound healing.					
36040455	6	42	theme	outstanding	1082:1092	arg1	performance					1146:1156	an outstanding accelerated infectious full-thickness wound-healing performance	1079:1156	an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue	1079:1279	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	4	43	with	matrix	721:726	arg1	interactions					739:750	tight interactions	733:750	tight interactions	733:750	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	4	43	with	matrix	721:726	arg1	bonding					772:778	hydrogen bonding	763:778	hydrogen bonding	763:778	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	4	43	with	matrix	721:726	arg1	interactions					797:808	coordination interactions	784:808	coordination interactions	784:808	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	5	44	dep	aureus	967:972	arg1	aureus					978:983	S. aureus	975:983	S. aureus	975:983	The decoration of ZnO endows the chitin/ZnO composite hydrogels with excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with acceptable biocompatibility.					
36040455	0	45	theme	infected	57:64	arg1	healing					72:78	infected wound healing	57:78	infected wound healing	57:78	Facile fabrication of chitin/ZnO composite hydrogels for infected wound healing.					
36040455	1	46	with	dressings	176:184	arg1	ability					210:216	antibacterial ability	196:216	antibacterial ability	196:216	When ordinary wounds are infected, the skin's self-healing capacity declines; thus appropriate dressings with both antibacterial ability and healing ability for bacteria-associated wounds are indispensable.					
36040455	1	46	with	dressings	176:184	arg1	ability					230:236	healing ability	222:236	healing ability	222:236	When ordinary wounds are infected, the skin's self-healing capacity declines; thus appropriate dressings with both antibacterial ability and healing ability for bacteria-associated wounds are indispensable.					
36040455	6	47	theme	composite	1054:1062	arg1	hydrogels					1064:1072	the chitin/ZnO composite hydrogels	1039:1072	the chitin/ZnO composite hydrogels	1039:1072	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	6	48	with	performance	1146:1156	arg1	proliferation					1179:1191	more fibroblast proliferation	1163:1191	more fibroblast proliferation	1163:1191	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	6	48	with	performance	1146:1156	arg1	neogenesis					1229:1238	more neogenesis	1224:1238	more neogenesis	1224:1238	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	6	48	with	performance	1146:1156	arg1	deposition					1208:1217	more collagen deposition	1194:1217	more collagen deposition	1194:1217	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	3	49	theme	ZnO	531:533	arg1	powders					535:541	commercial ZnO powders	520:541	commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration	520:611	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	5	50	theme	excellent	880:888	arg1	activity					904:911	excellent antibacterial activity	880:911	excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus)	880:984	The decoration of ZnO endows the chitin/ZnO composite hydrogels with excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with acceptable biocompatibility.					
36040455	3	51	theme	powders	535:541	arg1	addition					508:515	the sequential addition	493:515	the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration	493:611	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	6	52	dep	epithelium	1247:1256	arg1	the					1243:1245	the	1243:1245	the	1243:1245	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	1	53	theme	self-healing	127:138	arg1	capacity					140:147	the skin's self-healing capacity	116:147	the skin's self-healing capacity	116:147	When ordinary wounds are infected, the skin's self-healing capacity declines; thus appropriate dressings with both antibacterial ability and healing ability for bacteria-associated wounds are indispensable.					
36040455	5	54	theme	acceptable	992:1001	arg1	biocompatibility					1003:1018	acceptable biocompatibility	992:1018	acceptable biocompatibility	992:1018	The decoration of ZnO endows the chitin/ZnO composite hydrogels with excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with acceptable biocompatibility.					
36040455	6	55	theme	tissue	1274:1279	arg1	proliferation					1179:1191	more fibroblast proliferation	1163:1191	more fibroblast proliferation	1163:1191	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	6	55	theme	tissue	1274:1279	arg1	neogenesis					1229:1238	more neogenesis	1224:1238	more neogenesis	1224:1238	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	6	55	theme	tissue	1274:1279	arg1	deposition					1208:1217	more collagen deposition	1194:1217	more collagen deposition	1194:1217	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	1	56	theme	healing	222:228	arg1	ability					230:236	healing ability	222:236	healing ability	222:236	When ordinary wounds are infected, the skin's self-healing capacity declines; thus appropriate dressings with both antibacterial ability and healing ability for bacteria-associated wounds are indispensable.					
36040455	2	57	theme	full-thickness	383:396	arg1	material					417:424	an infected full-thickness skin wound-healing material	371:424	an infected full-thickness skin wound-healing material	371:424	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	2	57	theme	full-thickness	383:396	arg1	hydrogels					339:347	multifunctional chitin/ZnO composite hydrogels	302:347	multifunctional chitin/ZnO composite hydrogels	302:347	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	6	58	theme	wound-healing	1132:1144	arg1	performance					1146:1156	an outstanding accelerated infectious full-thickness wound-healing performance	1079:1156	an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue	1079:1279	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	5	59	theme	ZnO	829:831	arg1	decoration					815:824	The decoration	811:824	The decoration of ZnO	811:831	The decoration of ZnO endows the chitin/ZnO composite hydrogels with excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with acceptable biocompatibility.					
36040455	6	60	theme	more	1224:1227	arg1	neogenesis					1229:1238	more neogenesis	1224:1238	more neogenesis	1224:1238	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	5	61	dep	coli	933:936	arg1	coli					942:945	E. coli	939:945	E. coli	939:945	The decoration of ZnO endows the chitin/ZnO composite hydrogels with excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with acceptable biocompatibility.					
36040455	7	62	theme	infections	1428:1437	arg1	control					1417:1423	control	1417:1423	control	1417:1423	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	7	62	theme	infections	1428:1437	arg1	prevention					1402:1411	prevention	1402:1411	prevention	1402:1411	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	2	63	theme	skin	398:401	arg1	material					417:424	an infected full-thickness skin wound-healing material	371:424	an infected full-thickness skin wound-healing material	371:424	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	2	63	theme	skin	398:401	arg1	hydrogels					339:347	multifunctional chitin/ZnO composite hydrogels	302:347	multifunctional chitin/ZnO composite hydrogels	302:347	In this work, multifunctional chitin/ZnO composite hydrogels have been designed as an infected full-thickness skin wound-healing material.					
36040455	4	64	theme	chitin	714:719	arg1	matrix					721:726	the chitin matrix	710:726	the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions	710:808	The regenerated nanoscale ZnO particles aggregate into microscale particles and are embedded in the chitin matrix with tight interactions, including hydrogen bonding and coordination interactions.					
36040455	6	65	theme	full-thickness	1117:1130	arg1	performance					1146:1156	an outstanding accelerated infectious full-thickness wound-healing performance	1079:1156	an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue	1079:1279	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	0	66	theme	wound	66:70	arg1	healing					72:78	infected wound healing	57:78	infected wound healing	57:78	Facile fabrication of chitin/ZnO composite hydrogels for infected wound healing.					
36040455	6	67	theme	infectious	1106:1115	arg1	performance					1146:1156	an outstanding accelerated infectious full-thickness wound-healing performance	1079:1156	an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue	1079:1279	More importantly, the chitin/ZnO composite hydrogels show an outstanding accelerated infectious full-thickness wound-healing performance with more fibroblast proliferation, more collagen deposition, and more neogenesis of the epithelium and granulation tissue.					
36040455	3	68	theme	chitin	565:570	arg1	crosslinking					583:594	crosslinking	583:594	crosslinking	583:594	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	3	68	theme	chitin	565:570	arg1	solutions					572:580	aqueous alkaline chitin solutions	548:580	aqueous alkaline chitin solutions	548:580	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	3	68	theme	chitin	565:570	arg1	regeneration					600:611	regeneration	600:611	regeneration	600:611	The hydrogels are fabricated by a facile one-pot strategy through the sequential addition of commercial ZnO powders into aqueous alkaline chitin solutions, crosslinking and regeneration.					
36040455	7	69	theme	competitive	1361:1371	arg1	dressings					1384:1392	competitive skin wound dressings	1361:1392	competitive skin wound dressings for the prevention and control of infections	1361:1437	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	7	69	theme	competitive	1361:1371	arg1	hydrogels					1338:1346	the chitin/ZnO composite hydrogels	1313:1346	the chitin/ZnO composite hydrogels	1313:1346	Therefore, it is expected that the chitin/ZnO composite hydrogels can serve as competitive skin wound dressings for the prevention and control of infections.					
36040455	1	70	theme	bacteria-associated	242:260	arg1	wounds					262:267	bacteria-associated wounds	242:267	bacteria-associated wounds	242:267	When ordinary wounds are infected, the skin's self-healing capacity declines; thus appropriate dressings with both antibacterial ability and healing ability for bacteria-associated wounds are indispensable.					
37294939	7	0	theme	clinical	1208:1215	arg1	events					1200:1205	adverse events	1192:1205	adverse events (clinical and device-related adverse events)	1192:1250	Secondary outcomes included platelet recovery and function and adverse events (clinical and device-related adverse events) up to 30 days after surgery.					
37294939	7	0	theme	clinical	1208:1215	arg1	events					1244:1249	clinical and device-related adverse events	1208:1249	clinical and device-related adverse events	1208:1249	Secondary outcomes included platelet recovery and function and adverse events (clinical and device-related adverse events) up to 30 days after surgery.					
37294939	9	1	with	%	1488:1488	arg1	hematocrit					1510:1519	post-treatment hematocrit	1495:1519	post-treatment hematocrit of 41.8 (39.7-44.2) %	1495:1541	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	3	2	theme	free	635:638	arg1	hemoglobin					640:649	free hemoglobin	635:649	free hemoglobin	635:649	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	2	3	theme	filtration-based	344:359	arg1	device					304:309	The same™ device	294:309	The same™ device (i-SEP, France)	294:325	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	2	3	theme	filtration-based	344:359	arg1	device					377:382	an innovative filtration-based autotransfusion device	330:382	an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets	330:433	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	14	4	from	study	1940:1944	arg1	device					1958:1963	the sameTM device	1947:1963	the sameTM device	1947:1963	CONCLUSIONS In this first-in-human study, the sameTM device was able to simultaneously recover and wash both platelets and red blood cell.					
37294939	14	4	from	study	1940:1944	arg1	able					1969:1972	able	1969:1972	able	1969:1972	CONCLUSIONS In this first-in-human study, the sameTM device was able to simultaneously recover and wash both platelets and red blood cell.					
37294939	2	5	theme	same™	298:302	arg1	device					304:309	The same™ device	294:309	The same™ device (i-SEP, France)	294:325	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	2	5	theme	same™	298:302	arg1	device					377:382	an innovative filtration-based autotransfusion device	330:382	an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets	330:433	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	15	6	theme	higher	2101:2106	arg1	recovery					2117:2124	a higher platelet recovery	2099:2124	a higher platelet recovery of 52% with minimal platelet activation	2099:2164	Compared pre-clinical evaluations, the device achieved a higher platelet recovery of 52% with minimal platelet activation while maintaining platelet ability to be activated in vitro.					
37294939	9	7	theme	%	1541:1541	arg1	hematocrit					1510:1519	post-treatment hematocrit	1495:1519	post-treatment hematocrit of 41.8 (39.7-44.2) %	1495:1541	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	1	8	theme	BACKGROUND	180:189	arg1	devices					228:234	BACKGROUND Centrifugation-based autotransfusion devices	180:234	BACKGROUND Centrifugation-based autotransfusion devices	180:234	BACKGROUND Centrifugation-based autotransfusion devices only salvage red blood cells while platelets are removed.					
37294939	14	9	from	able	1969:1972	arg1	study					1940:1944	this first-in-human study	1920:1944	this first-in-human study	1920:1944	CONCLUSIONS In this first-in-human study, the sameTM device was able to simultaneously recover and wash both platelets and red blood cell.					
37294939	3	10	theme	post-treatment	541:554	arg1	hematocrit					556:565	a post-treatment hematocrit	539:565	a post-treatment hematocrit exceeding 40%	539:579	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	1	11	theme	autotransfusion	212:226	arg1	devices					228:234	BACKGROUND Centrifugation-based autotransfusion devices	180:234	BACKGROUND Centrifugation-based autotransfusion devices	180:234	BACKGROUND Centrifugation-based autotransfusion devices only salvage red blood cells while platelets are removed.					
37294939	11	12	theme	device	1663:1668	arg1	effect					1670:1675	No adverse device effect	1652:1675	No adverse device effect	1652:1675	No adverse device effect was reported.					
37294939	5	13	theme	residual	820:827	arg1	blood					852:856	residual cardiopulmonary bypass blood	820:856	residual cardiopulmonary bypass blood	820:856	The device was used intraoperatively to treat shed and residual cardiopulmonary bypass blood.					
37294939	8	14	theme	valve	1364:1368	arg1	surgery					1370:1376	26 (52%) valve surgery	1355:1376	26 (52%) valve surgery	1355:1376	RESULTS We included 50 patients of whom 18 (35%) underwent isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery.					
37294939	0	15	theme	non-comparative	139:153	arg1	study					155:159	a first-in-human non-comparative study	122:159	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).	0:178	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	0	15	theme	non-comparative	139:153	arg1	study					172:176	i-TRANSEP study	162:176	i-TRANSEP study	162:176	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	6	16	theme	recovery	903:910	arg1	performance					912:922	cell recovery performance	898:922	cell recovery performance	898:922	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	4	17	theme	elective	686:693	arg1	surgery					703:709	on-pump elective cardiac surgery	678:709	on-pump elective cardiac surgery	678:709	METHODS Adults undergoing on-pump elective cardiac surgery were included in a non-comparative multicenter trial.					
37294939	15	18	theme	minimal	2138:2144	arg1	activation					2155:2164	minimal platelet activation	2138:2164	minimal platelet activation	2138:2164	Compared pre-clinical evaluations, the device achieved a higher platelet recovery of 52% with minimal platelet activation while maintaining platelet ability to be activated in vitro.					
37294939	14	19	dep	wash	2004:2007	arg1	both					2009:2012	both	2009:2012	both	2009:2012	CONCLUSIONS In this first-in-human study, the sameTM device was able to simultaneously recover and wash both platelets and red blood cell.					
37294939	0	20	theme	on-pump	53:59	arg1	surgery					69:75	on-pump cardiac surgery	53:75	on-pump cardiac surgery using same™ by i-SEP autotransfusion device	53:119	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	4	21	theme	METHODS	652:658	arg1	Adults					660:665	METHODS Adults	652:665	METHODS Adults undergoing on-pump elective cardiac surgery	652:709	METHODS Adults undergoing on-pump elective cardiac surgery were included in a non-comparative multicenter trial.					
37294939	7	22	theme	adverse	1192:1198	arg1	events					1200:1205	adverse events	1192:1205	adverse events (clinical and device-related adverse events)	1192:1250	Secondary outcomes included platelet recovery and function and adverse events (clinical and device-related adverse events) up to 30 days after surgery.					
37294939	7	22	theme	adverse	1192:1198	arg1	events					1244:1249	clinical and device-related adverse events	1208:1249	clinical and device-related adverse events	1208:1249	Secondary outcomes included platelet recovery and function and adverse events (clinical and device-related adverse events) up to 30 days after surgery.					
37294939	9	23	theme	median	1415:1420	arg1	%					1488:1488	86.1 (25th-75th; 80.8-91.6) %	1460:1488	86.1 (25th-75th; 80.8-91.6) %	1460:1488	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	9	23	theme	median	1415:1420	arg1	recovery					1437:1444	The median red blood cell recovery	1411:1444	The median red blood cell recovery per cycle	1411:1454	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	8	24	theme	aortic	1390:1395	arg1	surgery					1402:1408	isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1340:1408	isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1340:1408	RESULTS We included 50 patients of whom 18 (35%) underwent isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery.					
37294939	14	25	theme	first-in-human	1925:1938	arg1	study					1940:1944	this first-in-human study	1920:1944	this first-in-human study	1920:1944	CONCLUSIONS In this first-in-human study, the sameTM device was able to simultaneously recover and wash both platelets and red blood cell.					
37294939	13	26	theme	flow	1854:1857	arg1	cytometry					1859:1867	flow cytometry	1854:1867	flow cytometry	1854:1867	Platelet activation state and function, evaluated by flow cytometry, was found unaltered by the device.					
37294939	9	27	theme	blood	1426:1430	arg1	%					1488:1488	86.1 (25th-75th; 80.8-91.6) %	1460:1488	86.1 (25th-75th; 80.8-91.6) %	1460:1488	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	9	27	theme	blood	1426:1430	arg1	recovery					1437:1444	The median red blood cell recovery	1411:1444	The median red blood cell recovery per cycle	1411:1454	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	6	28	theme	red	951:953	arg1	cell					961:964	red blood cell recovery and post-treatment hematocrit	951:1003	cell	961:964	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	4	29	theme	multicenter	746:756	arg1	trial					758:762	a non-comparative multicenter trial	728:762	a non-comparative multicenter trial	728:762	METHODS Adults undergoing on-pump elective cardiac surgery were included in a non-comparative multicenter trial.					
37294939	2	30	theme	red	405:407	arg1	cells					415:419	red blood cells	405:419	red blood cells	405:419	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	0	31	theme	i-SEP	92:96	arg1	device					114:119	i-SEP autotransfusion device	92:119	i-SEP autotransfusion device	92:119	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	0	32	theme	Combined	0:7	arg1	platelet					9:16	Combined platelet	0:16	Combined platelet	0:16	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	7	33	theme	Secondary	1129:1137	arg1	outcomes					1139:1146	Secondary outcomes	1129:1146	Secondary outcomes	1129:1146	Secondary outcomes included platelet recovery and function and adverse events (clinical and device-related adverse events) up to 30 days after surgery.					
37294939	12	34	theme	platelet	1698:1705	arg1	recovery					1707:1714	Median platelet recovery	1691:1714	Median platelet recovery	1691:1714	Median platelet recovery was 52.4 (44.2-60.1) %, with a post-treatment concentration of 116 (93-146) 109.L-1.					
37294939	9	35	theme	86.1	1460:1463	arg1	%					1488:1488	86.1 (25th-75th; 80.8-91.6) %	1460:1488	86.1 (25th-75th; 80.8-91.6) %	1460:1488	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	9	35	theme	86.1	1460:1463	arg1	recovery					1437:1444	The median red blood cell recovery	1411:1444	The median red blood cell recovery per cycle	1411:1454	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	6	36	theme	biological	1013:1022	arg1	safety					1024:1029	biological safety	1013:1029	biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios	1013:1126	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	3	37	theme	heparin	616:622	arg1	heparin					616:622	heparin	616:622	heparin	616:622	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	3	37	theme	heparin	616:622	arg1	%					630:630	75%	628:630	75% of free hemoglobin	628:649	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	3	37	theme	heparin	616:622	arg1	%					611:611	more than 90%	599:611	more than 90% of heparin	599:622	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	3	37	theme	heparin	616:622	arg1	hemoglobin					640:649	free hemoglobin	635:649	free hemoglobin	635:649	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	5	38	dep	blood	852:856	arg1	bypass					845:850	bypass	845:850	bypass	845:850	The device was used intraoperatively to treat shed and residual cardiopulmonary bypass blood.					
37294939	0	39	theme	blood	26:30	arg1	cell					32:35	red blood cell	22:35	red blood cell	22:35	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	12	40	with	%	1737:1737	arg1	concentration					1762:1774	a post-treatment concentration	1745:1774	a post-treatment concentration of 116 (93-146) 109.L-1	1745:1798	Median platelet recovery was 52.4 (44.2-60.1) %, with a post-treatment concentration of 116 (93-146) 109.L-1.					
37294939	15	41	theme	pre-clinical	2053:2064	arg1	evaluations					2066:2076	pre-clinical evaluations	2053:2076	pre-clinical evaluations	2053:2076	Compared pre-clinical evaluations, the device achieved a higher platelet recovery of 52% with minimal platelet activation while maintaining platelet ability to be activated in vitro.					
37294939	8	42	theme	CABG	1349:1352	arg1	surgery					1402:1408	isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1340:1408	isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1340:1408	RESULTS We included 50 patients of whom 18 (35%) underwent isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery.					
37294939	2	43	theme	innovative	333:342	arg1	device					304:309	The same™ device	294:309	The same™ device (i-SEP, France)	294:325	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	2	43	theme	innovative	333:342	arg1	device					377:382	an innovative filtration-based autotransfusion device	330:382	an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets	330:433	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	6	44	theme	free	1084:1087	arg1	hemoglobin					1089:1098	free hemoglobin	1084:1098	free hemoglobin expressed as removal ratios	1084:1126	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	8	45	theme	26	1355:1356	arg1	surgery					1370:1376	26 (52%) valve surgery	1355:1376	26 (52%) valve surgery	1355:1376	RESULTS We included 50 patients of whom 18 (35%) underwent isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery.					
37294939	15	46	with	recovery	2117:2124	arg1	activation					2155:2164	minimal platelet activation	2138:2164	minimal platelet activation	2138:2164	Compared pre-clinical evaluations, the device achieved a higher platelet recovery of 52% with minimal platelet activation while maintaining platelet ability to be activated in vitro.					
37294939	15	47	theme	%	2131:2131	arg1	recovery					2117:2124	a higher platelet recovery	2099:2124	a higher platelet recovery of 52% with minimal platelet activation	2099:2164	Compared pre-clinical evaluations, the device achieved a higher platelet recovery of 52% with minimal platelet activation while maintaining platelet ability to be activated in vitro.					
37294939	6	48	theme	heparin	1072:1078	arg1	washout					1061:1067	the washout	1057:1067	the washout of heparin and free hemoglobin expressed as removal ratios	1057:1126	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	13	49	theme	activation	1810:1819	arg1	state					1821:1825	Platelet activation state	1801:1825	Platelet activation state	1801:1825	Platelet activation state and function, evaluated by flow cytometry, was found unaltered by the device.					
37294939	3	50	theme	cell	506:509	arg1	recovery					511:518	a red blood cell recovery	494:518	a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%	494:579	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	1	51	theme	blood	253:257	arg1	cells					259:263	red blood cells	249:263	red blood cells	249:263	BACKGROUND Centrifugation-based autotransfusion devices only salvage red blood cells while platelets are removed.					
37294939	6	52	theme	cell	898:901	arg1	performance					912:922	cell recovery performance	898:922	cell recovery performance	898:922	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	9	53	theme	post-treatment	1495:1508	arg1	hematocrit					1510:1519	post-treatment hematocrit	1495:1519	post-treatment hematocrit of 41.8 (39.7-44.2) %	1495:1541	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	7	54	theme	adverse	1236:1242	arg1	events					1200:1205	adverse events	1192:1205	adverse events (clinical and device-related adverse events)	1192:1250	Secondary outcomes included platelet recovery and function and adverse events (clinical and device-related adverse events) up to 30 days after surgery.					
37294939	7	54	theme	adverse	1236:1242	arg1	events					1244:1249	clinical and device-related adverse events	1208:1249	clinical and device-related adverse events	1208:1249	Secondary outcomes included platelet recovery and function and adverse events (clinical and device-related adverse events) up to 30 days after surgery.					
37294939	14	55	theme	red	2028:2030	arg1	cell					2038:2041	red blood cell	2028:2041	red blood cell	2028:2041	CONCLUSIONS In this first-in-human study, the sameTM device was able to simultaneously recover and wash both platelets and red blood cell.					
37294939	2	56	theme	autotransfusion	361:375	arg1	device					304:309	The same™ device	294:309	The same™ device (i-SEP, France)	294:325	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	2	56	theme	autotransfusion	361:375	arg1	device					377:382	an innovative filtration-based autotransfusion device	330:382	an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets	330:433	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	15	57	theme	platelet	2108:2115	arg1	recovery					2117:2124	a higher platelet recovery	2099:2124	a higher platelet recovery of 52% with minimal platelet activation	2099:2164	Compared pre-clinical evaluations, the device achieved a higher platelet recovery of 52% with minimal platelet activation while maintaining platelet ability to be activated in vitro.					
37294939	3	58	theme	hemoglobin	640:649	arg1	heparin					616:622	heparin	616:622	heparin	616:622	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	3	58	theme	hemoglobin	640:649	arg1	%					630:630	75%	628:630	75% of free hemoglobin	628:649	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	3	58	theme	hemoglobin	640:649	arg1	%					611:611	more than 90%	599:611	more than 90% of heparin	599:622	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	3	58	theme	hemoglobin	640:649	arg1	hemoglobin					640:649	free hemoglobin	635:649	free hemoglobin	635:649	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	14	59	dep	CONCLUSIONS	1905:1915	arg1	device					1958:1963	the sameTM device	1947:1963	the sameTM device	1947:1963	CONCLUSIONS In this first-in-human study, the sameTM device was able to simultaneously recover and wash both platelets and red blood cell.					
37294939	14	59	dep	CONCLUSIONS	1905:1915	arg1	able					1969:1972	able	1969:1972	able	1969:1972	CONCLUSIONS In this first-in-human study, the sameTM device was able to simultaneously recover and wash both platelets and red blood cell.					
37294939	2	60	dep	device	304:309	arg1	i-SEP					312:316	i-SEP	312:316	i-SEP	312:316	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	2	60	dep	device	304:309	arg1	France					319:324	France	319:324	France	319:324	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	5	61	theme	cardiopulmonary	829:843	arg1	blood					852:856	residual cardiopulmonary bypass blood	820:856	residual cardiopulmonary bypass blood	820:856	The device was used intraoperatively to treat shed and residual cardiopulmonary bypass blood.					
37294939	6	62	theme	primary	863:869	arg1	outcome					871:877	The primary outcome	859:877	The primary outcome	859:877	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	6	62	theme	primary	863:869	arg1	composite					885:893	a composite	883:893	a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios	883:1126	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	6	62	theme	primary	863:869	arg1	safety					1024:1029	biological safety	1013:1029	biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios	1013:1126	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	6	62	theme	primary	863:869	arg1	performance					912:922	cell recovery performance	898:922	cell recovery performance	898:922	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	6	63	theme	removal	1113:1119	arg1	ratios					1121:1126	removal ratios	1113:1126	removal ratios	1113:1126	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	9	64	dep	25th-75th	1466:1474	arg1	80.8-91.6					1477:1485	80.8-91.6	1477:1485	80.8-91.6	1477:1485	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	1	65	theme	Centrifugation-based	191:210	arg1	devices					228:234	BACKGROUND Centrifugation-based autotransfusion devices	180:234	BACKGROUND Centrifugation-based autotransfusion devices	180:234	BACKGROUND Centrifugation-based autotransfusion devices only salvage red blood cells while platelets are removed.					
37294939	12	66	theme	109.L-1	1792:1798	arg1	concentration					1762:1774	a post-treatment concentration	1745:1774	a post-treatment concentration of 116 (93-146) 109.L-1	1745:1798	Median platelet recovery was 52.4 (44.2-60.1) %, with a post-treatment concentration of 116 (93-146) 109.L-1.					
37294939	2	67	theme	able	384:387	arg1	device					304:309	The same™ device	294:309	The same™ device (i-SEP, France)	294:325	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	2	67	theme	able	384:387	arg1	device					377:382	an innovative filtration-based autotransfusion device	330:382	an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets	330:433	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	3	68	theme	red	496:498	arg1	recovery					511:518	a red blood cell recovery	494:518	a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%	494:579	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	0	69	theme	first-in-human	124:137	arg1	study					155:159	a first-in-human non-comparative study	122:159	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).	0:178	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	0	69	theme	first-in-human	124:137	arg1	study					172:176	i-TRANSEP study	162:176	i-TRANSEP study	162:176	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	8	70	theme	surgery	1370:1376	arg1	surgery					1402:1408	isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1340:1408	isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1340:1408	RESULTS We included 50 patients of whom 18 (35%) underwent isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery.					
37294939	14	71	theme	sameTM	1951:1956	arg1	device					1958:1963	the sameTM device	1947:1963	the sameTM device	1947:1963	CONCLUSIONS In this first-in-human study, the sameTM device was able to simultaneously recover and wash both platelets and red blood cell.					
37294939	14	71	theme	sameTM	1951:1956	arg1	able					1969:1972	able	1969:1972	able	1969:1972	CONCLUSIONS In this first-in-human study, the sameTM device was able to simultaneously recover and wash both platelets and red blood cell.					
37294939	15	72	theme	platelet	2184:2191	arg1	ability					2193:2199	platelet ability	2184:2199	platelet ability to be activated in vitro	2184:2224	Compared pre-clinical evaluations, the device achieved a higher platelet recovery of 52% with minimal platelet activation while maintaining platelet ability to be activated in vitro.					
37294939	12	73	theme	Median	1691:1696	arg1	recovery					1707:1714	Median platelet recovery	1691:1714	Median platelet recovery	1691:1714	Median platelet recovery was 52.4 (44.2-60.1) %, with a post-treatment concentration of 116 (93-146) 109.L-1.					
37294939	6	74	dep	cell	961:964	arg1	recovery					966:973	recovery	966:973	recovery	966:973	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	6	75	theme	performance	912:922	arg1	outcome					871:877	The primary outcome	859:877	The primary outcome	859:877	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	6	75	theme	performance	912:922	arg1	composite					885:893	a composite	883:893	a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios	883:1126	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	6	75	theme	performance	912:922	arg1	safety					1024:1029	biological safety	1013:1029	biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios	1013:1126	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	6	75	theme	performance	912:922	arg1	performance					912:922	cell recovery performance	898:922	cell recovery performance	898:922	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	8	76	theme	6	1382:1382	arg1	surgery					1402:1408	isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1340:1408	isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1340:1408	RESULTS We included 50 patients of whom 18 (35%) underwent isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery.					
37294939	11	77	theme	adverse	1655:1661	arg1	effect					1670:1675	No adverse device effect	1652:1675	No adverse device effect	1652:1675	No adverse device effect was reported.					
37294939	0	78	theme	i-TRANSEP	162:170	arg1	study					155:159	a first-in-human non-comparative study	122:159	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).	0:178	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	0	78	theme	i-TRANSEP	162:170	arg1	study					172:176	i-TRANSEP study	162:176	i-TRANSEP study	162:176	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	4	79	theme	on-pump	678:684	arg1	surgery					703:709	on-pump elective cardiac surgery	678:709	on-pump elective cardiac surgery	678:709	METHODS Adults undergoing on-pump elective cardiac surgery were included in a non-comparative multicenter trial.					
37294939	0	80	theme	cardiac	61:67	arg1	surgery					69:75	on-pump cardiac surgery	53:75	on-pump cardiac surgery using same™ by i-SEP autotransfusion device	53:119	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	15	81	theme	platelet	2146:2153	arg1	activation					2155:2164	minimal platelet activation	2138:2164	minimal platelet activation	2138:2164	Compared pre-clinical evaluations, the device achieved a higher platelet recovery of 52% with minimal platelet activation while maintaining platelet ability to be activated in vitro.					
37294939	6	82	theme	post-treatment	979:992	arg1	hematocrit					994:1003	post-treatment hematocrit	979:1003	post-treatment hematocrit	979:1003	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	9	83	dep	86.1	1460:1463	arg1	25th-75th					1466:1474	25th-75th	1466:1474	25th-75th; 80.8-91.6	1466:1485	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	8	84	theme	root	1397:1400	arg1	surgery					1402:1408	isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1340:1408	isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1340:1408	RESULTS We included 50 patients of whom 18 (35%) underwent isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery.					
37294939	12	85	theme	post-treatment	1747:1760	arg1	concentration					1762:1774	a post-treatment concentration	1745:1774	a post-treatment concentration of 116 (93-146) 109.L-1	1745:1798	Median platelet recovery was 52.4 (44.2-60.1) %, with a post-treatment concentration of 116 (93-146) 109.L-1.					
37294939	9	86	theme	red	1422:1424	arg1	%					1488:1488	86.1 (25th-75th; 80.8-91.6) %	1460:1488	86.1 (25th-75th; 80.8-91.6) %	1460:1488	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	9	86	theme	red	1422:1424	arg1	recovery					1437:1444	The median red blood cell recovery	1411:1444	The median red blood cell recovery per cycle	1411:1454	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	6	87	theme	blood	955:959	arg1	cell					961:964	red blood cell recovery and post-treatment hematocrit	951:1003	cell	961:964	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	0	88	theme	autotransfusion	98:112	arg1	device					114:119	i-SEP autotransfusion device	92:119	i-SEP autotransfusion device	92:119	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	9	89	theme	cell	1432:1435	arg1	%					1488:1488	86.1 (25th-75th; 80.8-91.6) %	1460:1488	86.1 (25th-75th; 80.8-91.6) %	1460:1488	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	9	89	theme	cell	1432:1435	arg1	recovery					1437:1444	The median red blood cell recovery	1411:1444	The median red blood cell recovery per cycle	1411:1454	The median red blood cell recovery per cycle was 86.1 (25th-75th; 80.8-91.6) % with post-treatment hematocrit of 41.8 (39.7-44.2) %.					
37294939	4	90	theme	non-comparative	730:744	arg1	trial					758:762	a non-comparative multicenter trial	728:762	a non-comparative multicenter trial	728:762	METHODS Adults undergoing on-pump elective cardiac surgery were included in a non-comparative multicenter trial.					
37294939	7	91	theme	platelet	1157:1164	arg1	recovery					1166:1173	platelet recovery	1157:1173	platelet recovery	1157:1173	Secondary outcomes included platelet recovery and function and adverse events (clinical and device-related adverse events) up to 30 days after surgery.					
37294939	4	92	theme	cardiac	695:701	arg1	surgery					703:709	on-pump elective cardiac surgery	678:709	on-pump elective cardiac surgery	678:709	METHODS Adults undergoing on-pump elective cardiac surgery were included in a non-comparative multicenter trial.					
37294939	0	93	theme	red	22:24	arg1	cell					32:35	red blood cell	22:35	red blood cell	22:35	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	6	94	theme	safety	1024:1029	arg1	outcome					871:877	The primary outcome	859:877	The primary outcome	859:877	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	6	94	theme	safety	1024:1029	arg1	composite					885:893	a composite	883:893	a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios	883:1126	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	6	94	theme	safety	1024:1029	arg1	safety					1024:1029	biological safety	1013:1029	biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios	1013:1126	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	6	94	theme	safety	1024:1029	arg1	performance					912:922	cell recovery performance	898:922	cell recovery performance	898:922	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	3	95	theme	new	471:473	arg1	device					475:480	this new device	466:480	this new device	466:480	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	2	96	theme	blood	409:413	arg1	cells					415:419	red blood cells	405:419	red blood cells	405:419	The same™ device (i-SEP, France) is an innovative filtration-based autotransfusion device able to salvage both red blood cells and platelets.					
37294939	8	97	dep	RESULTS	1281:1287	arg1	included					1292:1299	included	1292:1299	included 50 patients of whom 18 (35%) underwent isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1292:1408	RESULTS We included 50 patients of whom 18 (35%) underwent isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery.					
37294939	6	98	theme	hemoglobin	1089:1098	arg1	washout					1061:1067	the washout	1057:1067	the washout of heparin and free hemoglobin expressed as removal ratios	1057:1126	The primary outcome was a composite of cell recovery performance, assessed in the device by red blood cell recovery and post-treatment hematocrit, and of biological safety assessed in the device by the washout of heparin and free hemoglobin expressed as removal ratios.					
37294939	7	99	dep	recovery	1166:1173	arg1	days					1261:1264	up to 30 days	1252:1264	up to 30 days after surgery	1252:1278	Secondary outcomes included platelet recovery and function and adverse events (clinical and device-related adverse events) up to 30 days after surgery.					
37294939	8	100	theme	isolated	1340:1347	arg1	surgery					1402:1408	isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1340:1408	isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery	1340:1408	RESULTS We included 50 patients of whom 18 (35%) underwent isolated CABG, 26 (52%) valve surgery and 6 (12%) aortic root surgery.					
37294939	3	101	theme	blood	500:504	arg1	recovery					511:518	a red blood cell recovery	494:518	a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%	494:579	We tested the hypothesis that this new device could allow a red blood cell recovery exceeding 80% with a post-treatment hematocrit exceeding 40%, and would remove more than 90% of heparin and 75% of free hemoglobin.					
37294939	5	102	used	used	780:783	arg2	device					769:774	The device	765:774	The device	765:774	The device was used intraoperatively to treat shed and residual cardiopulmonary bypass blood.					
37294939	7	103	dep	30	1258:1259	arg1	to					1255:1256	to	1255:1256	to	1255:1256	Secondary outcomes included platelet recovery and function and adverse events (clinical and device-related adverse events) up to 30 days after surgery.					
37294939	13	104	theme	Platelet	1801:1808	arg1	state					1821:1825	Platelet activation state	1801:1825	Platelet activation state	1801:1825	Platelet activation state and function, evaluated by flow cytometry, was found unaltered by the device.					
37294939	10	105	theme	Removal	1544:1550	arg1	ratios					1552:1557	Removal ratios	1544:1557	Removal ratios for heparin and free-hemoglobin	1544:1589	Removal ratios for heparin and free-hemoglobin were 98.9 (98.2-99.7) % and 94.6 (92.7-96.6) % respectively.					
37294939	1	106	theme	red	249:251	arg1	cells					259:263	red blood cells	249:263	red blood cells	249:263	BACKGROUND Centrifugation-based autotransfusion devices only salvage red blood cells while platelets are removed.					
37294939	0	107	dep	platelet	9:16	arg1	study					155:159	a first-in-human non-comparative study	122:159	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).	0:178	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	0	107	dep	platelet	9:16	arg1	study					172:176	i-TRANSEP study	162:176	i-TRANSEP study	162:176	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	0	107	dep	platelet	9:16	arg1	recovery					37:44	recovery	37:44	recovery	37:44	Combined platelet and red blood cell recovery during on-pump cardiac surgery using same™ by i-SEP autotransfusion device: a first-in-human non-comparative study (i-TRANSEP study).					
37294939	7	108	theme	device-related	1221:1234	arg1	events					1200:1205	adverse events	1192:1205	adverse events (clinical and device-related adverse events)	1192:1250	Secondary outcomes included platelet recovery and function and adverse events (clinical and device-related adverse events) up to 30 days after surgery.					
37294939	7	108	theme	device-related	1221:1234	arg1	events					1244:1249	clinical and device-related adverse events	1208:1249	clinical and device-related adverse events	1208:1249	Secondary outcomes included platelet recovery and function and adverse events (clinical and device-related adverse events) up to 30 days after surgery.					
37294939	14	109	theme	blood	2032:2036	arg1	cell					2038:2041	red blood cell	2028:2041	red blood cell	2028:2041	CONCLUSIONS In this first-in-human study, the sameTM device was able to simultaneously recover and wash both platelets and red blood cell.					
35608970	4	0	theme	rRNA	278:281	arg1	analysis					297:304	16S rRNA gene sequence analysis	274:304	16S rRNA gene sequence analysis	274:304	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	6	1	theme	B.	1021:1022	arg1	asteroides					1024:1033	B. asteroides	1021:1033	B. asteroides	1021:1033	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	10	2	theme	%	1714:1714	arg1	dDDH					1716:1719	41.9 % dDDH	1709:1719	41.9 % dDDH	1709:1719	Strain S053-2T shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with B. asteroides DSM 20089T.					
35608970	10	3	theme	41.9 	1709:1713	arg1	%					1714:1714	%	1714:1714	%	1714:1714	Strain S053-2T shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with B. asteroides DSM 20089T.					
35608970	7	4	theme	94.5-95.3	1055:1063	arg1	%					1064:1064	%	1064:1064	%	1064:1064	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	10	5	theme	AAI	1732:1734	arg1	values					1736:1741	AAI values	1732:1741	AAI values	1732:1741	Strain S053-2T shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with B. asteroides DSM 20089T.					
35608970	5	6	theme	16S	701:703	arg1	analysis					724:731	the 16S rRNA gene sequence analysis	697:731	the 16S rRNA gene sequence analysis	697:731	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	12	7	from	d-mannose	2059:2067	arg1	production					2043:2052	Acid production	2038:2052	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase	2038:2143	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase could differentiate strain S053-2T from B. apousia W8102T.					
35608970	12	8	from	melezitose	2088:2097	arg1	production					2043:2052	Acid production	2038:2052	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase	2038:2143	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase could differentiate strain S053-2T from B. apousia W8102T.					
35608970	2	9	theme	designated	112:121	arg1	bifidobacteria					96:109	A novel bifidobacteria	88:109	A novel bifidobacteria (designated S053-2T)	88:130	A novel bifidobacteria (designated S053-2T) was isolated from the gut of honeybee (Apis mellifera).					
35608970	2	9	theme	designated	112:121	arg1	S053-2T					123:129	designated S053-2T	112:129	designated S053-2T	112:129	A novel bifidobacteria (designated S053-2T) was isolated from the gut of honeybee (Apis mellifera).					
35608970	12	10	from	activity	2120:2127	arg1	production					2043:2052	Acid production	2038:2052	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase	2038:2143	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase could differentiate strain S053-2T from B. apousia W8102T.					
35608970	7	11	theme	dnaK	1084:1087	arg1	similarities					1107:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities	1055:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia	1055:1151	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	6	12	theme	low	890:892	arg1	pheS					908:911	relatively low (91.6-95.7 %) pheS	879:911	relatively low (91.6-95.7 %) pheS	879:911	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	12	theme	low	890:892	arg1	hsp60					950:954	hsp60	950:954	hsp60	950:954	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	12	theme	low	890:892	arg1	atpA					914:917	atpA	914:917	atpA	914:917	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	12	theme	low	890:892	arg1	fusA					932:935	fusA	932:935	fusA	932:935	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	12	theme	low	890:892	arg1	similarities					986:997	recA sequence similarities	972:997	recA sequence similarities	972:997	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	12	theme	low	890:892	arg1	glnA					938:941	glnA	938:941	glnA	938:941	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	12	theme	low	890:892	arg1	argS					957:960	argS	957:960	argS	957:960	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	12	theme	low	890:892	arg1	clpC					920:923	clpC	920:923	clpC	920:923	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	12	theme	low	890:892	arg1	pyrG					963:966	pyrG	963:966	pyrG	963:966	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	12	theme	low	890:892	arg1	dnaG					926:929	dnaG	926:929	dnaG	926:929	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	12	theme	low	890:892	arg1	glyS					944:947	glyS	944:947	glyS	944:947	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	3	13	theme	Strain	188:193	arg1	S053-2T					195:201	Strain S053-2T	188:201	Strain S053-2T	188:201	Strain S053-2T was characterized using a polyphasic taxonomic approach.					
35608970	4	14	theme	strain	321:326	arg1	S053-2T					328:334	strain S053-2T	321:334	strain S053-2T	321:334	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	11	15	theme	DSM	2026:2028	arg1	20089T					2030:2035	B. asteroides DSM 20089T	2012:2035	B. asteroides DSM 20089T	2012:2035	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	8	16	from	species	1441:1447	arg1	group					1470:1474	the B. asteroides group	1452:1474	the B. asteroides group	1452:1474	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	14	17	theme	AB	2371:2372	arg1	S053-2T					2344:2350	S053-2T	2344:2350	S053-2T (=JCM 34710T=CCTCC AB 2021129T)	2344:2382	nov., is proposed, and the type strain is S053-2T (=JCM 34710T=CCTCC AB 2021129T).					
35608970	14	17	theme	AB	2371:2372	arg1	2021129T					2374:2381	=JCM 34710T=CCTCC AB 2021129T	2353:2381	=JCM 34710T=CCTCC AB 2021129T	2353:2381	nov., is proposed, and the type strain is S053-2T (=JCM 34710T=CCTCC AB 2021129T).					
35608970	3	18	theme	polyphasic	229:238	arg1	approach					250:257	a polyphasic taxonomic approach	227:257	a polyphasic taxonomic approach	227:257	Strain S053-2T was characterized using a polyphasic taxonomic approach.					
35608970	8	19	theme	phylogenomic	1158:1169	arg1	tree					1171:1174	The phylogenomic tree	1154:1174	The phylogenomic tree	1154:1174	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	5	20	theme	strain	734:739	arg1	S053-2T					741:747	strain S053-2T	734:747	strain S053-2T	734:747	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	5	21	theme	sequence	839:846	arg1	similarity					848:857	99.7 % 16S rRNA gene sequence similarity	818:857	99.7 % 16S rRNA gene sequence similarity	818:857	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	14	22	theme	=JCM	2353:2356	arg1	S053-2T					2344:2350	S053-2T	2344:2350	S053-2T (=JCM 34710T=CCTCC AB 2021129T)	2344:2382	nov., is proposed, and the type strain is S053-2T (=JCM 34710T=CCTCC AB 2021129T).					
35608970	14	22	theme	=JCM	2353:2356	arg1	2021129T					2374:2381	=JCM 34710T=CCTCC AB 2021129T	2353:2381	=JCM 34710T=CCTCC AB 2021129T	2353:2381	nov., is proposed, and the type strain is S053-2T (=JCM 34710T=CCTCC AB 2021129T).					
35608970	12	23	from	W8102T	2196:2201	arg1	S053-2T					2172:2178	strain S053-2T	2165:2178	strain S053-2T from B. apousia W8102T	2165:2201	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase could differentiate strain S053-2T from B. apousia W8102T.					
35608970	4	24	contain	had	635:637	arg1	S053-2T					328:334	strain S053-2T	321:334	strain S053-2T	321:334	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	4	24	contain	had	635:637	arg2	similarities					674:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	4	25	theme	indicum	432:438	arg1	strains					377:383	the type strains	368:383	the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum	368:628	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	8	26	theme	strain	1191:1196	arg1	S053-2T					1198:1204	strain S053-2T	1191:1204	strain S053-2T	1191:1204	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	5	27	theme	gene	710:713	arg1	analysis					724:731	the 16S rRNA gene sequence analysis	697:731	the 16S rRNA gene sequence analysis	697:731	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	2	28	theme	Apis	171:174	arg1	honeybee					161:168	honeybee	161:168	honeybee (Apis mellifera)	161:185	A novel bifidobacteria (designated S053-2T) was isolated from the gut of honeybee (Apis mellifera).					
35608970	2	28	theme	Apis	171:174	arg1	mellifera					176:184	Apis mellifera	171:184	Apis mellifera	171:184	A novel bifidobacteria (designated S053-2T) was isolated from the gut of honeybee (Apis mellifera).					
35608970	6	29	contain	had	875:877	arg2	pyrG					963:966	pyrG	963:966	pyrG	963:966	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	29	contain	had	875:877	arg2	clpC					920:923	clpC	920:923	clpC	920:923	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	29	contain	had	875:877	arg2	pheS					908:911	relatively low (91.6-95.7 %) pheS	879:911	relatively low (91.6-95.7 %) pheS	879:911	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	29	contain	had	875:877	arg2	dnaG					926:929	dnaG	926:929	dnaG	926:929	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	29	contain	had	875:877	arg2	glyS					944:947	glyS	944:947	glyS	944:947	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	29	contain	had	875:877	arg2	atpA					914:917	atpA	914:917	atpA	914:917	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	29	contain	had	875:877	arg2	fusA					932:935	fusA	932:935	fusA	932:935	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	29	contain	had	875:877	arg2	hsp60					950:954	hsp60	950:954	hsp60	950:954	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	29	contain	had	875:877	arg2	glnA					938:941	glnA	938:941	glnA	938:941	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	29	contain	had	875:877	arg1	S053-2T					867:873	Strain S053-2T	860:873	Strain S053-2T	860:873	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	29	contain	had	875:877	arg2	argS					957:960	argS	957:960	argS	957:960	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	29	contain	had	875:877	arg2	similarities					986:997	recA sequence similarities	972:997	recA sequence similarities	972:997	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	11	30	from	maltose	1852:1858	arg1	production					1779:1788	Acid production	1774:1788	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase	1774:1970	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	9	31	dep	identity	1622:1629	arg1	%					1641:1641	95.6%	1637:1641	95.6%	1637:1641	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	9	31	dep	identity	1622:1629	arg1	AAI					1632:1634	AAI	1632:1634	AAI	1632:1634	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	11	32	theme	Acid	1774:1777	arg1	production					1779:1788	Acid production	1774:1788	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase	1774:1970	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	5	33	theme	type	781:784	arg1	strain					786:791	the type strain	777:791	the type strain of B. asteroides	777:808	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	7	34	theme	dnaG	1078:1081	arg1	similarities					1107:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities	1055:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia	1055:1151	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	9	35	theme	DNA-DNA	1562:1568	arg1	hybridization					1570:1582	digital DNA-DNA hybridization	1554:1582	digital DNA-DNA hybridization (dDDH, 52.4 %)	1554:1597	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	9	36	theme	average	1603:1609	arg1	identity					1622:1629	average amino acid identity	1603:1629	average amino acid identity (AAI, 95.6%)	1603:1642	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	4	37	theme	xylocopae	493:501	arg1	strains					377:383	the type strains	368:383	the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum	368:628	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	11	38	from	amygdalin	1829:1837	arg1	production					1779:1788	Acid production	1774:1788	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase	1774:1970	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	12	39	from	maltose	2070:2076	arg1	production					2043:2052	Acid production	2038:2052	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase	2038:2143	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase could differentiate strain S053-2T from B. apousia W8102T.					
35608970	9	40	theme	acid	1617:1620	arg1	identity					1622:1629	average amino acid identity	1603:1629	average amino acid identity (AAI, 95.6%)	1603:1642	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	4	41	theme	Bifidobacterium	504:518	arg1	coryneforme					520:530	Bifidobacterium coryneforme	504:530	Bifidobacterium coryneforme	504:530	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	7	42	contain	had	1051:1053	arg1	S053-2T					1043:1049	Strain S053-2T	1036:1049	Strain S053-2T	1036:1049	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	7	42	contain	had	1051:1053	arg2	similarities					1107:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities	1055:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia	1055:1151	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	11	43	from	cellobiose	1840:1849	arg1	production					1779:1788	Acid production	1774:1788	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase	1774:1970	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	44	theme	strain	1992:1997	arg1	S053-2T					1999:2005	strain S053-2T	1992:2005	strain S053-2T	1992:2005	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	4	45	theme	Bifidobacterium	441:455	arg1	actinocoloniiforme					457:474	Bifidobacterium actinocoloniiforme	441:474	Bifidobacterium actinocoloniiforme	441:474	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	7	46	theme	pyrG	1093:1096	arg1	similarities					1107:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities	1055:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia	1055:1151	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	1	47	theme	honeybee	61:68	arg1	gut					54:56	the gut	50:56	the gut of honeybee (Apis mellifera)	50:85	nov., isolated from the gut of honeybee (Apis mellifera).					
35608970	6	48	theme	type	1006:1009	arg1	strain					1011:1016	the type strain	1002:1016	the type strain of B. asteroides	1002:1033	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	10	49	dep	B.	1748:1749	arg1	asteroides					1751:1760	asteroides	1751:1760	asteroides	1751:1760	Strain S053-2T shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with B. asteroides DSM 20089T.					
35608970	9	50	dep	hybridization	1570:1582	arg1	dDDH					1585:1588	dDDH	1585:1588	dDDH	1585:1588	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	9	50	dep	hybridization	1570:1582	arg1	%					1596:1596	52.4 %	1591:1596	52.4 %	1591:1596	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	5	51	theme	%	823:823	arg1	similarity					848:857	99.7 % 16S rRNA gene sequence similarity	818:857	99.7 % 16S rRNA gene sequence similarity	818:857	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	1	52	theme	Apis	71:74	arg1	honeybee					61:68	honeybee	61:68	honeybee (Apis mellifera)	61:85	nov., isolated from the gut of honeybee (Apis mellifera).					
35608970	1	52	theme	Apis	71:74	arg1	mellifera					76:84	Apis mellifera	71:84	Apis mellifera	71:84	nov., isolated from the gut of honeybee (Apis mellifera).					
35608970	5	53	theme	rRNA	829:832	arg1	similarity					848:857	99.7 % 16S rRNA gene sequence similarity	818:857	99.7 % 16S rRNA gene sequence similarity	818:857	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	8	54	dep	B.	1345:1346	arg1	polysaccharolyticum					1348:1366	polysaccharolyticum	1348:1366	polysaccharolyticum	1348:1366	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	6	55	theme	sequence	977:984	arg1	pheS					908:911	relatively low (91.6-95.7 %) pheS	879:911	relatively low (91.6-95.7 %) pheS	879:911	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	55	theme	sequence	977:984	arg1	similarities					986:997	recA sequence similarities	972:997	recA sequence similarities	972:997	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	8	56	theme	species	1441:1447	arg1	strains					1399:1405	type strains	1394:1405	type strains of other phylogenetically related species in the B. asteroides group	1394:1474	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	10	57	theme	Strain	1675:1680	arg1	S053-2T					1682:1688	Strain S053-2T	1675:1688	Strain S053-2T	1675:1688	Strain S053-2T shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with B. asteroides DSM 20089T.					
35608970	8	58	theme	type	1279:1282	arg1	strains					1284:1290	the type strains	1275:1290	the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group	1275:1474	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	5	59	theme	asteroides	799:808	arg1	strain					786:791	the type strain	777:791	the type strain of B. asteroides	777:808	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	7	60	theme	clpC	1072:1075	arg1	similarities					1107:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities	1055:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia	1055:1151	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	8	61	dep	B.	1222:1223	arg1	asteroides					1225:1234	asteroides	1225:1234	asteroides	1225:1234	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	4	62	theme	analysis	297:304	arg1	result					264:269	The result	260:269	The result of 16S rRNA gene sequence analysis	260:304	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	2	63	attach	isolated	136:143	arg2	S053-2T					123:129	designated S053-2T	112:129	designated S053-2T	112:129	A novel bifidobacteria (designated S053-2T) was isolated from the gut of honeybee (Apis mellifera).					
35608970	2	63	attach	isolated	136:143	arg2	bifidobacteria					96:109	A novel bifidobacteria	88:109	A novel bifidobacteria (designated S053-2T)	88:130	A novel bifidobacteria (designated S053-2T) was isolated from the gut of honeybee (Apis mellifera).					
35608970	2	63	attach	isolated	136:143	arg1	gut					154:156	the gut	150:156	the gut of honeybee (Apis mellifera)	150:185	A novel bifidobacteria (designated S053-2T) was isolated from the gut of honeybee (Apis mellifera).					
35608970	11	64	theme	lipase	1943:1948	arg1	l-arabinose					1795:1805	l-arabinose	1795:1805	l-arabinose	1795:1805	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	64	theme	lipase	1943:1948	arg1	amygdalin					1829:1837	amygdalin	1829:1837	amygdalin	1829:1837	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	64	theme	lipase	1943:1948	arg1	l-fucose					1908:1915	l-fucose	1908:1915	l-fucose	1908:1915	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	64	theme	lipase	1943:1948	arg1	cellobiose					1840:1849	cellobiose	1840:1849	cellobiose	1840:1849	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	64	theme	lipase	1943:1948	arg1	sucrose					1872:1878	sucrose	1872:1878	sucrose	1872:1878	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	64	theme	lipase	1943:1948	arg1	d-xylose					1808:1815	d-xylose	1808:1815	d-xylose	1808:1815	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	64	theme	lipase	1943:1948	arg1	raffinose					1881:1889	raffinose	1881:1889	raffinose	1881:1889	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	64	theme	lipase	1943:1948	arg1	d-mannose					1818:1826	d-mannose	1818:1826	d-mannose	1818:1826	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	64	theme	lipase	1943:1948	arg1	activity					1922:1929	activity	1922:1929	activity	1922:1929	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	64	theme	lipase	1943:1948	arg1	maltose					1852:1858	maltose	1852:1858	maltose	1852:1858	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	64	theme	lipase	1943:1948	arg1	melibiose					1861:1869	melibiose	1861:1869	melibiose	1861:1869	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	64	theme	lipase	1943:1948	arg1	α-fucosidase					1959:1970	α-fucosidase	1959:1970	α-fucosidase	1959:1970	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	64	theme	lipase	1943:1948	arg1	gentiobiose					1892:1902	gentiobiose	1892:1902	gentiobiose	1892:1902	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	7	65	theme	atpA	1066:1069	arg1	similarities					1107:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities	1055:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia	1055:1151	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	13	66	theme	novel	2257:2261	arg1	species					2263:2269	a novel species	2255:2269	a novel species	2255:2269	Based upon the data obtained in the present study, a novel species, Bifidobacterium mizhiense sp.					
35608970	13	66	theme	novel	2257:2261	arg1	data					2219:2222	the data	2215:2222	the data obtained in the present study	2215:2252	Based upon the data obtained in the present study, a novel species, Bifidobacterium mizhiense sp.					
35608970	11	67	from	d-xylose	1808:1815	arg1	production					1779:1788	Acid production	1774:1788	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase	1774:1970	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	8	68	theme	asteroides	1459:1468	arg1	group					1470:1474	the B. asteroides group	1452:1474	the B. asteroides group	1452:1474	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	2	69	theme	honeybee	161:168	arg1	gut					154:156	the gut	150:156	the gut of honeybee (Apis mellifera)	150:185	A novel bifidobacteria (designated S053-2T) was isolated from the gut of honeybee (Apis mellifera).					
35608970	8	70	theme	asteroides	1298:1307	arg1	strains					1284:1290	the type strains	1275:1290	the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group	1275:1474	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	9	71	theme	Strain	1477:1482	arg1	S053-2T					1484:1490	Strain S053-2T	1477:1490	Strain S053-2T	1477:1490	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	9	72	theme	highest	1503:1509	arg1	ANI					1540:1542	ANI	1540:1542	ANI	1540:1542	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	9	72	theme	highest	1503:1509	arg1	identity					1530:1537	the highest average nucleotide identity	1499:1537	the highest average nucleotide identity (ANI, 93.8 %)	1499:1551	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	4	73	theme	16S	274:276	arg1	analysis					297:304	16S rRNA gene sequence analysis	274:304	16S rRNA gene sequence analysis	274:304	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	4	74	theme	choladohabitans	574:588	arg1	strains					377:383	the type strains	368:383	the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum	368:628	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	11	75	from	d-mannose	1818:1826	arg1	production					1779:1788	Acid production	1774:1788	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase	1774:1970	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	6	76	dep	low	890:892	arg1	%					905:905	91.6-95.7 %	895:905	91.6-95.7 %	895:905	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	8	77	from	strains	1399:1405	arg1	group					1470:1474	the B. asteroides group	1452:1474	the B. asteroides group	1452:1474	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	9	78	theme	nucleotide	1519:1528	arg1	ANI					1540:1542	ANI	1540:1542	ANI	1540:1542	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	9	78	theme	nucleotide	1519:1528	arg1	identity					1530:1537	the highest average nucleotide identity	1499:1537	the highest average nucleotide identity (ANI, 93.8 %)	1499:1551	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	4	79	theme	gene	283:286	arg1	analysis					297:304	16S rRNA gene sequence analysis	274:304	16S rRNA gene sequence analysis	274:304	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	6	80	theme	asteroides	1024:1033	arg1	strain					1011:1016	the type strain	1002:1016	the type strain of B. asteroides	1002:1033	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	4	81	theme	16S	651:653	arg1	similarities					674:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	2	82	theme	novel	90:94	arg1	bifidobacteria					96:109	A novel bifidobacteria	88:109	A novel bifidobacteria (designated S053-2T)	88:130	A novel bifidobacteria (designated S053-2T) was isolated from the gut of honeybee (Apis mellifera).					
35608970	2	82	theme	novel	90:94	arg1	S053-2T					123:129	designated S053-2T	112:129	designated S053-2T	112:129	A novel bifidobacteria (designated S053-2T) was isolated from the gut of honeybee (Apis mellifera).					
35608970	13	83	theme	Bifidobacterium	2272:2286	arg1	sp					2298:2299	Bifidobacterium mizhiense sp	2272:2299	Bifidobacterium mizhiense sp	2272:2299	Based upon the data obtained in the present study, a novel species, Bifidobacterium mizhiense sp.					
35608970	13	83	theme	Bifidobacterium	2272:2286	arg1	data					2219:2222	the data	2215:2222	the data obtained in the present study	2215:2252	Based upon the data obtained in the present study, a novel species, Bifidobacterium mizhiense sp.					
35608970	13	84	theme	mizhiense	2288:2296	arg1	sp					2298:2299	Bifidobacterium mizhiense sp	2272:2299	Bifidobacterium mizhiense sp	2272:2299	Based upon the data obtained in the present study, a novel species, Bifidobacterium mizhiense sp.					
35608970	13	84	theme	mizhiense	2288:2296	arg1	data					2219:2222	the data	2215:2222	the data obtained in the present study	2215:2252	Based upon the data obtained in the present study, a novel species, Bifidobacterium mizhiense sp.					
35608970	12	85	theme	Acid	2038:2041	arg1	production					2043:2052	Acid production	2038:2052	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase	2038:2143	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase could differentiate strain S053-2T from B. apousia W8102T.					
35608970	7	86	theme	B.	1142:1143	arg1	apousia					1145:1151	B. apousia	1142:1151	B. apousia	1142:1151	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	11	87	from	l-arabinose	1795:1805	arg1	production					1779:1788	Acid production	1774:1788	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase	1774:1970	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	4	88	theme	polysaccharolyticum	610:628	arg1	strains					377:383	the type strains	368:383	the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum	368:628	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	7	89	theme	Strain	1036:1041	arg1	S053-2T					1043:1049	Strain S053-2T	1036:1049	Strain S053-2T	1036:1049	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	4	90	theme	type	372:375	arg1	strains					377:383	the type strains	368:383	the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum	368:628	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	8	91	from	group	1470:1474	arg1	strains					1399:1405	type strains	1394:1405	type strains of other phylogenetically related species in the B. asteroides group	1394:1474	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	11	92	theme	asteroides	2015:2024	arg1	20089T					2030:2035	B. asteroides DSM 20089T	2012:2035	B. asteroides DSM 20089T	2012:2035	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	10	93	theme	B.	1748:1749	arg1	20089T					1766:1771	B. asteroides DSM 20089T	1748:1771	B. asteroides DSM 20089T	1748:1771	Strain S053-2T shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with B. asteroides DSM 20089T.					
35608970	5	94	theme	gene	834:837	arg1	similarity					848:857	99.7 % 16S rRNA gene sequence similarity	818:857	99.7 % 16S rRNA gene sequence similarity	818:857	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	14	95	theme	34710T=CCTCC	2358:2369	arg1	S053-2T					2344:2350	S053-2T	2344:2350	S053-2T (=JCM 34710T=CCTCC AB 2021129T)	2344:2382	nov., is proposed, and the type strain is S053-2T (=JCM 34710T=CCTCC AB 2021129T).					
35608970	14	95	theme	34710T=CCTCC	2358:2369	arg1	2021129T					2374:2381	=JCM 34710T=CCTCC AB 2021129T	2353:2381	=JCM 34710T=CCTCC AB 2021129T	2353:2381	nov., is proposed, and the type strain is S053-2T (=JCM 34710T=CCTCC AB 2021129T).					
35608970	3	96	theme	taxonomic	240:248	arg1	approach					250:257	a polyphasic taxonomic approach	227:257	a polyphasic taxonomic approach	227:257	Strain S053-2T was characterized using a polyphasic taxonomic approach.					
35608970	4	97	theme	gene	660:663	arg1	similarities					674:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	4	98	theme	apousia	549:555	arg1	strains					377:383	the type strains	368:383	the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum	368:628	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	5	99	theme	rRNA	705:708	arg1	analysis					724:731	the 16S rRNA gene sequence analysis	697:731	the 16S rRNA gene sequence analysis	697:731	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	9	100	theme	digital	1554:1560	arg1	hybridization					1570:1582	digital DNA-DNA hybridization	1554:1582	digital DNA-DNA hybridization (dDDH, 52.4 %)	1554:1597	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	11	101	from	α-fucosidase	1959:1970	arg1	production					1779:1788	Acid production	1774:1788	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase	1774:1970	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	10	102	theme	DSM	1762:1764	arg1	20089T					1766:1771	B. asteroides DSM 20089T	1748:1771	B. asteroides DSM 20089T	1748:1771	Strain S053-2T shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with B. asteroides DSM 20089T.					
35608970	5	103	theme	sequence	715:722	arg1	analysis					724:731	the 16S rRNA gene sequence analysis	697:731	the 16S rRNA gene sequence analysis	697:731	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	14	104	theme	type	2329:2332	arg1	S053-2T					2344:2350	S053-2T	2344:2350	S053-2T (=JCM 34710T=CCTCC AB 2021129T)	2344:2382	nov., is proposed, and the type strain is S053-2T (=JCM 34710T=CCTCC AB 2021129T).					
35608970	14	104	theme	type	2329:2332	arg1	strain					2334:2339	the type strain	2325:2339	the type strain	2325:2339	nov., is proposed, and the type strain is S053-2T (=JCM 34710T=CCTCC AB 2021129T).					
35608970	4	105	theme	asteroides	404:413	arg1	strains					377:383	the type strains	368:383	the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum	368:628	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	7	106	theme	type	1127:1130	arg1	strain					1132:1137	the type strain	1123:1137	the type strain of B. apousia	1123:1151	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	4	107	theme	sequence	665:672	arg1	similarities					674:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	9	108	theme	amino	1611:1615	arg1	identity					1622:1629	average amino acid identity	1603:1629	average amino acid identity (AAI, 95.6%)	1603:1642	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	4	109	theme	coryneforme	520:530	arg1	strains					377:383	the type strains	368:383	the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum	368:628	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	8	110	theme	B.	1222:1223	arg1	group					1236:1240	the B. asteroides group	1218:1240	the B. asteroides group	1218:1240	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	7	111	theme	sequence	1098:1105	arg1	similarities					1107:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities	1055:1118	94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia	1055:1151	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	9	112	dep	identity	1530:1537	arg1	values					1644:1649	values	1644:1649	values	1644:1649	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	11	113	theme	B.	2012:2013	arg1	20089T					2030:2035	B. asteroides DSM 20089T	2012:2035	B. asteroides DSM 20089T	2012:2035	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	4	114	theme	actinocoloniiforme	457:474	arg1	strains					377:383	the type strains	368:383	the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum	368:628	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	8	115	theme	other	1410:1414	arg1	species					1441:1447	other phylogenetically related species	1410:1447	other phylogenetically related species in the B. asteroides group	1410:1474	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	13	116	theme	present	2240:2246	arg1	study					2248:2252	the present study	2236:2252	the present study	2236:2252	Based upon the data obtained in the present study, a novel species, Bifidobacterium mizhiense sp.					
35608970	4	117	theme	95.5-99.7 	639:648	arg1	similarities					674:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	5	118	theme	99.7 	818:822	arg1	%					823:823	%	823:823	%	823:823	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	8	119	theme	type	1394:1397	arg1	strains					1399:1405	type strains	1394:1405	type strains of other phylogenetically related species in the B. asteroides group	1394:1474	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	11	120	theme	esterase	1934:1941	arg1	C8					1951:1952	C8	1951:1952	C8	1951:1952	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	11	120	theme	esterase	1934:1941	arg1	lipase					1943:1948	esterase lipase	1934:1948	esterase lipase (C8)	1934:1953	Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T.					
35608970	8	121	theme	related	1433:1439	arg1	species					1441:1447	other phylogenetically related species	1410:1447	other phylogenetically related species in the B. asteroides group	1410:1474	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	5	122	theme	16S	825:827	arg1	similarity					848:857	99.7 % 16S rRNA gene sequence similarity	818:857	99.7 % 16S rRNA gene sequence similarity	818:857	Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides, having 99.7 % 16S rRNA gene sequence similarity.					
35608970	12	123	theme	α-fucosidase	2132:2143	arg1	production					2043:2052	Acid production	2038:2052	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase	2038:2143	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase could differentiate strain S053-2T from B. apousia W8102T.					
35608970	12	124	from	sucrose	2079:2085	arg1	production					2043:2052	Acid production	2038:2052	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase	2038:2143	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase could differentiate strain S053-2T from B. apousia W8102T.					
35608970	6	125	theme	recA	972:975	arg1	pheS					908:911	relatively low (91.6-95.7 %) pheS	879:911	relatively low (91.6-95.7 %) pheS	879:911	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	6	125	theme	recA	972:975	arg1	similarities					986:997	recA sequence similarities	972:997	recA sequence similarities	972:997	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	8	126	theme	B.	1456:1457	arg1	group					1470:1474	the B. asteroides group	1452:1474	the B. asteroides group	1452:1474	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	12	127	theme	strain	2165:2170	arg1	S053-2T					2172:2178	strain S053-2T	2165:2178	strain S053-2T from B. apousia W8102T	2165:2201	Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase could differentiate strain S053-2T from B. apousia W8102T.					
35608970	7	128	theme	%	1064:1064	arg1	atpA					1066:1069	94.5-95.3% atpA	1055:1069	94.5-95.3% atpA	1055:1069	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	8	129	dep	B.	1322:1323	arg1	choladohabitans					1325:1339	choladohabitans	1325:1339	choladohabitans	1325:1339	The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum, and distantly related to type strains of other phylogenetically related species in the B. asteroides group.					
35608970	6	130	theme	Strain	860:865	arg1	S053-2T					867:873	Strain S053-2T	860:873	Strain S053-2T	860:873	Strain S053-2T had relatively low (91.6-95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides.					
35608970	4	131	theme	sequence	288:295	arg1	analysis					297:304	16S rRNA gene sequence analysis	274:304	16S rRNA gene sequence analysis	274:304	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	4	132	theme	%	649:649	arg1	similarities					674:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	9	133	theme	average	1511:1517	arg1	ANI					1540:1542	ANI	1540:1542	ANI	1540:1542	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	9	133	theme	average	1511:1517	arg1	identity					1530:1537	the highest average nucleotide identity	1499:1537	the highest average nucleotide identity (ANI, 93.8 %)	1499:1551	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
35608970	7	134	theme	apousia	1145:1151	arg1	strain					1132:1137	the type strain	1123:1137	the type strain of B. apousia	1123:1151	Strain S053-2T had 94.5-95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia.					
35608970	4	135	theme	rRNA	655:658	arg1	similarities					674:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	95.5-99.7 % 16S rRNA gene sequence similarities	639:685	The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae, Bifidobacterium coryneforme, Bifidobacterium apousia, Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum, and had 95.5-99.7 % 16S rRNA gene sequence similarities.					
35608970	10	136	dep	%	1702:1702	arg1	values					1736:1741	AAI values	1732:1741	AAI values	1732:1741	Strain S053-2T shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with B. asteroides DSM 20089T.					
35608970	10	136	dep	%	1702:1702	arg1	ANI					1704:1706	ANI	1704:1706	91.1 % ANI	1697:1706	Strain S053-2T shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with B. asteroides DSM 20089T.					
35608970	9	137	dep	ANI	1540:1542	arg1	%					1550:1550	93.8 %	1545:1550	93.8 %	1545:1550	Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T.					
36460156	4	0	from	change	690:695	arg1	rejection					715:723	rejection	715:723	rejection	715:723	The optimized membrane exhibited slight change in water flux and rejection under chlorine environment.					
36460156	4	0	from	change	690:695	arg1	flux					706:709	water flux	700:709	water flux	700:709	The optimized membrane exhibited slight change in water flux and rejection under chlorine environment.					
36460156	8	1	theme	reacquired	1336:1345	arg1	membrane					1360:1367	the reacquired β-CD2/10/PES membrane	1332:1367	the reacquired β-CD2/10/PES membrane into fresh azobenzene labeled zwitterions solution	1332:1418	The antifouling membrane can be regenerated via immersing the reacquired β-CD2/10/PES membrane into fresh azobenzene labeled zwitterions solution again.					
36460156	8	2	theme	zwitterions	1399:1409	arg1	solution					1411:1418	fresh azobenzene labeled zwitterions solution	1374:1418	fresh azobenzene labeled zwitterions solution	1374:1418	The antifouling membrane can be regenerated via immersing the reacquired β-CD2/10/PES membrane into fresh azobenzene labeled zwitterions solution again.					
36460156	4	3	theme	optimized	654:662	arg1	membrane					664:671	The optimized membrane	650:671	The optimized membrane	650:671	The optimized membrane exhibited slight change in water flux and rejection under chlorine environment.					
36460156	6	4	theme	cis	996:998	arg1	isomers					1000:1006	the cis isomers	992:1006	the cis isomers	992:1006	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	2	5	theme	interfacial	396:406	arg1	polymerization					408:421	interfacial polymerization	396:421	interfacial polymerization using beta cyclodextrin (β-CD) monomers	396:461	In this study, a composite nanofiltration (NF) membrane (β-CDx/y/PES) was prepared by interfacial polymerization using beta cyclodextrin (β-CD) monomers.					
36460156	5	6	from	loss	815:818	arg1	flux					829:832	water flux	823:832	water flux	823:832	The AZ-β-CDx/y/PES membrane also displayed an evidently lower loss in water flux in the antifouling test in comparison with the β-CDx/y/PES membrane.					
36460156	0	7	theme	cyclodextrin	132:143	arg1	monomers					145:152	beta cyclodextrin monomers	127:152	beta cyclodextrin monomers	127:152	Preparation of chlorine-resistant and regenerable antifouling nanofiltration membrane through interfacial polymerization using beta cyclodextrin monomers.					
36460156	2	8	theme	NF	353:354	arg1	membrane					357:364	a composite nanofiltration (NF) membrane	325:364	a composite nanofiltration (NF) membrane (β-CDx/y/PES)	325:378	In this study, a composite nanofiltration (NF) membrane (β-CDx/y/PES) was prepared by interfacial polymerization using beta cyclodextrin (β-CD) monomers.					
36460156	2	8	theme	NF	353:354	arg1	β-CDx/y/PES					367:377	β-CDx/y/PES	367:377	β-CDx/y/PES	367:377	In this study, a composite nanofiltration (NF) membrane (β-CDx/y/PES) was prepared by interfacial polymerization using beta cyclodextrin (β-CD) monomers.					
36460156	3	9	theme	host-guest	626:635	arg1	interaction					637:647	host-guest interaction	626:647	host-guest interaction	626:647	Subsequently, the β-CD-based (AZ-β-CDx/y/PES) membrane was prepared by assembling azobenzene labeled zwitterions into the hydrophobic internal cavity of β-CD via host-guest interaction.					
36460156	0	10	theme	beta	127:130	arg1	monomers					145:152	beta cyclodextrin monomers	127:152	beta cyclodextrin monomers	127:152	Preparation of chlorine-resistant and regenerable antifouling nanofiltration membrane through interfacial polymerization using beta cyclodextrin monomers.					
36460156	3	11	theme	β-CD	617:620	arg1	cavity					607:612	the hydrophobic internal cavity	582:612	the hydrophobic internal cavity of β-CD	582:620	Subsequently, the β-CD-based (AZ-β-CDx/y/PES) membrane was prepared by assembling azobenzene labeled zwitterions into the hydrophobic internal cavity of β-CD via host-guest interaction.					
36460156	6	12	theme	light	1061:1065	arg1	irradiation					1067:1077	the UV light irradiation	1054:1077	the UV light irradiation	1054:1077	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	3	13	theme	azobenzene	546:555	arg1	zwitterions					565:575	azobenzene labeled zwitterions	546:575	azobenzene labeled zwitterions	546:575	Subsequently, the β-CD-based (AZ-β-CDx/y/PES) membrane was prepared by assembling azobenzene labeled zwitterions into the hydrophobic internal cavity of β-CD via host-guest interaction.					
36460156	5	14	with	comparison	861:870	arg1	membrane					893:900	the β-CDx/y/PES membrane	877:900	the β-CDx/y/PES membrane	877:900	The AZ-β-CDx/y/PES membrane also displayed an evidently lower loss in water flux in the antifouling test in comparison with the β-CDx/y/PES membrane.					
36460156	2	15	theme	nanofiltration	337:350	arg1	membrane					357:364	a composite nanofiltration (NF) membrane	325:364	a composite nanofiltration (NF) membrane (β-CDx/y/PES)	325:378	In this study, a composite nanofiltration (NF) membrane (β-CDx/y/PES) was prepared by interfacial polymerization using beta cyclodextrin (β-CD) monomers.					
36460156	2	15	theme	nanofiltration	337:350	arg1	β-CDx/y/PES					367:377	β-CDx/y/PES	367:377	β-CDx/y/PES	367:377	In this study, a composite nanofiltration (NF) membrane (β-CDx/y/PES) was prepared by interfacial polymerization using beta cyclodextrin (β-CD) monomers.					
36460156	8	16	theme	antifouling	1278:1288	arg1	membrane					1290:1297	The antifouling membrane	1274:1297	The antifouling membrane	1274:1297	The antifouling membrane can be regenerated via immersing the reacquired β-CD2/10/PES membrane into fresh azobenzene labeled zwitterions solution again.					
36460156	5	17	theme	β-CDx/y/PES	881:891	arg1	membrane					893:900	the β-CDx/y/PES membrane	877:900	the β-CDx/y/PES membrane	877:900	The AZ-β-CDx/y/PES membrane also displayed an evidently lower loss in water flux in the antifouling test in comparison with the β-CDx/y/PES membrane.					
36460156	7	18	theme	membrane	1187:1194	arg1	surface					1196:1202	the membrane surface	1183:1202	the membrane surface	1183:1202	Hence, the contaminants upon the membrane surface can be simply eliminated by water washing under UV light irradiation.					
36460156	2	19	theme	composite	327:335	arg1	membrane					357:364	a composite nanofiltration (NF) membrane	325:364	a composite nanofiltration (NF) membrane (β-CDx/y/PES)	325:378	In this study, a composite nanofiltration (NF) membrane (β-CDx/y/PES) was prepared by interfacial polymerization using beta cyclodextrin (β-CD) monomers.					
36460156	2	19	theme	composite	327:335	arg1	β-CDx/y/PES					367:377	β-CDx/y/PES	367:377	β-CDx/y/PES	367:377	In this study, a composite nanofiltration (NF) membrane (β-CDx/y/PES) was prepared by interfacial polymerization using beta cyclodextrin (β-CD) monomers.					
36460156	2	20	theme	β-CD	448:451	arg1	monomers					454:461	beta cyclodextrin (β-CD) monomers	429:461	beta cyclodextrin (β-CD) monomers	429:461	In this study, a composite nanofiltration (NF) membrane (β-CDx/y/PES) was prepared by interfacial polymerization using beta cyclodextrin (β-CD) monomers.					
36460156	7	21	theme	light	1255:1259	arg1	irradiation					1261:1271	UV light irradiation	1252:1271	UV light irradiation	1252:1271	Hence, the contaminants upon the membrane surface can be simply eliminated by water washing under UV light irradiation.					
36460156	8	22	theme	β-CD2/10/PES	1347:1358	arg1	membrane					1360:1367	the reacquired β-CD2/10/PES membrane	1332:1367	the reacquired β-CD2/10/PES membrane into fresh azobenzene labeled zwitterions solution	1332:1418	The antifouling membrane can be regenerated via immersing the reacquired β-CD2/10/PES membrane into fresh azobenzene labeled zwitterions solution again.					
36460156	6	23	theme	azobenzene	1113:1122	arg1	zwitterions					1132:1142	azobenzene labeled zwitterions	1113:1142	azobenzene labeled zwitterions	1113:1142	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	6	24	theme	UV	1058:1059	arg1	irradiation					1067:1077	the UV light irradiation	1054:1077	the UV light irradiation	1054:1077	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	1	25	with	membrane	168:175	arg1	resistance					196:205	good chlorine resistance	182:205	good chlorine resistance	182:205	Constructing membrane with good chlorine resistance and antifouling properties is considered to be important challenges confronting membrane applications.					
36460156	1	25	with	membrane	168:175	arg1	properties					223:232	antifouling properties	211:232	antifouling properties	211:232	Constructing membrane with good chlorine resistance and antifouling properties is considered to be important challenges confronting membrane applications.					
36460156	6	26	theme	azo	933:935	arg1	groups					937:942	the trans azo groups	923:942	the trans azo groups in azobenzene labeled zwitterions	923:976	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	2	27	theme	cyclodextrin	434:445	arg1	monomers					454:461	beta cyclodextrin (β-CD) monomers	429:461	beta cyclodextrin (β-CD) monomers	429:461	In this study, a composite nanofiltration (NF) membrane (β-CDx/y/PES) was prepared by interfacial polymerization using beta cyclodextrin (β-CD) monomers.					
36460156	0	28	theme	chlorine-resistant	15:32	arg1	membrane					77:84	chlorine-resistant and regenerable antifouling nanofiltration membrane	15:84	chlorine-resistant and regenerable antifouling nanofiltration membrane	15:84	Preparation of chlorine-resistant and regenerable antifouling nanofiltration membrane through interfacial polymerization using beta cyclodextrin monomers.					
36460156	3	29	theme	internal	598:605	arg1	cavity					607:612	the hydrophobic internal cavity	582:612	the hydrophobic internal cavity of β-CD	582:620	Subsequently, the β-CD-based (AZ-β-CDx/y/PES) membrane was prepared by assembling azobenzene labeled zwitterions into the hydrophobic internal cavity of β-CD via host-guest interaction.					
36460156	6	30	theme	labeled	958:964	arg1	zwitterions					966:976	azobenzene labeled zwitterions	947:976	azobenzene labeled zwitterions	947:976	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	1	31	theme	antifouling	211:221	arg1	properties					223:232	antifouling properties	211:232	antifouling properties	211:232	Constructing membrane with good chlorine resistance and antifouling properties is considered to be important challenges confronting membrane applications.					
36460156	1	32	theme	important	254:262	arg1	challenges					264:273	important challenges	254:273	important challenges confronting membrane applications	254:307	Constructing membrane with good chlorine resistance and antifouling properties is considered to be important challenges confronting membrane applications.					
36460156	0	33	theme	antifouling	50:60	arg1	membrane					77:84	chlorine-resistant and regenerable antifouling nanofiltration membrane	15:84	chlorine-resistant and regenerable antifouling nanofiltration membrane	15:84	Preparation of chlorine-resistant and regenerable antifouling nanofiltration membrane through interfacial polymerization using beta cyclodextrin monomers.					
36460156	8	34	theme	labeled	1391:1397	arg1	solution					1411:1418	fresh azobenzene labeled zwitterions solution	1374:1418	fresh azobenzene labeled zwitterions solution	1374:1418	The antifouling membrane can be regenerated via immersing the reacquired β-CD2/10/PES membrane into fresh azobenzene labeled zwitterions solution again.					
36460156	5	35	theme	lower	809:813	arg1	loss					815:818	an evidently lower loss	796:818	an evidently lower loss in water flux	796:832	The AZ-β-CDx/y/PES membrane also displayed an evidently lower loss in water flux in the antifouling test in comparison with the β-CDx/y/PES membrane.					
36460156	6	36	theme	azobenzene	947:956	arg1	zwitterions					966:976	azobenzene labeled zwitterions	947:976	azobenzene labeled zwitterions	947:976	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	3	37	theme	β-CD-based	482:491	arg1	membrane					510:517	the β-CD-based (AZ-β-CDx/y/PES) membrane	478:517	the β-CD-based (AZ-β-CDx/y/PES) membrane	478:517	Subsequently, the β-CD-based (AZ-β-CDx/y/PES) membrane was prepared by assembling azobenzene labeled zwitterions into the hydrophobic internal cavity of β-CD via host-guest interaction.					
36460156	3	37	theme	β-CD-based	482:491	arg1	AZ-β-CDx/y/PES					494:507	AZ-β-CDx/y/PES	494:507	AZ-β-CDx/y/PES	494:507	Subsequently, the β-CD-based (AZ-β-CDx/y/PES) membrane was prepared by assembling azobenzene labeled zwitterions into the hydrophobic internal cavity of β-CD via host-guest interaction.					
36460156	8	38	theme	azobenzene	1380:1389	arg1	solution					1411:1418	fresh azobenzene labeled zwitterions solution	1374:1418	fresh azobenzene labeled zwitterions solution	1374:1418	The antifouling membrane can be regenerated via immersing the reacquired β-CD2/10/PES membrane into fresh azobenzene labeled zwitterions solution again.					
36460156	0	39	theme	regenerable	38:48	arg1	membrane					77:84	chlorine-resistant and regenerable antifouling nanofiltration membrane	15:84	chlorine-resistant and regenerable antifouling nanofiltration membrane	15:84	Preparation of chlorine-resistant and regenerable antifouling nanofiltration membrane through interfacial polymerization using beta cyclodextrin monomers.					
36460156	6	40	theme	labeled	1124:1130	arg1	zwitterions					1132:1142	azobenzene labeled zwitterions	1113:1142	azobenzene labeled zwitterions	1113:1142	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	0	41	theme	membrane	77:84	arg1	Preparation					0:10	Preparation	0:10	Preparation of chlorine-resistant and regenerable antifouling nanofiltration membrane through interfacial polymerization using beta cyclodextrin monomers.	0:153	Preparation of chlorine-resistant and regenerable antifouling nanofiltration membrane through interfacial polymerization using beta cyclodextrin monomers.					
36460156	6	42	theme	light	1023:1027	arg1	irradiation					1029:1039	the visible light irradiation	1011:1039	the visible light irradiation converted to the UV light irradiation	1011:1077	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	5	43	theme	AZ-β-CDx/y/PES	757:770	arg1	membrane					772:779	The AZ-β-CDx/y/PES membrane	753:779	The AZ-β-CDx/y/PES membrane	753:779	The AZ-β-CDx/y/PES membrane also displayed an evidently lower loss in water flux in the antifouling test in comparison with the β-CDx/y/PES membrane.					
36460156	1	44	theme	Constructing	155:166	arg1	membrane					168:175	Constructing membrane	155:175	Constructing membrane with good chlorine resistance and antifouling properties	155:232	Constructing membrane with good chlorine resistance and antifouling properties is considered to be important challenges confronting membrane applications.					
36460156	0	45	theme	nanofiltration	62:75	arg1	membrane					77:84	chlorine-resistant and regenerable antifouling nanofiltration membrane	15:84	chlorine-resistant and regenerable antifouling nanofiltration membrane	15:84	Preparation of chlorine-resistant and regenerable antifouling nanofiltration membrane through interfacial polymerization using beta cyclodextrin monomers.					
36460156	6	46	theme	visible	1015:1021	arg1	irradiation					1029:1039	the visible light irradiation	1011:1039	the visible light irradiation converted to the UV light irradiation	1011:1077	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	5	47	from	test	853:856	arg1	comparison					861:870	comparison	861:870	comparison with the β-CDx/y/PES membrane	861:900	The AZ-β-CDx/y/PES membrane also displayed an evidently lower loss in water flux in the antifouling test in comparison with the β-CDx/y/PES membrane.					
36460156	6	48	theme	trans	927:931	arg1	groups					937:942	the trans azo groups	923:942	the trans azo groups in azobenzene labeled zwitterions	923:976	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	3	49	theme	labeled	557:563	arg1	zwitterions					565:575	azobenzene labeled zwitterions	546:575	azobenzene labeled zwitterions	546:575	Subsequently, the β-CD-based (AZ-β-CDx/y/PES) membrane was prepared by assembling azobenzene labeled zwitterions into the hydrophobic internal cavity of β-CD via host-guest interaction.					
36460156	1	50	theme	chlorine	187:194	arg1	resistance					196:205	good chlorine resistance	182:205	good chlorine resistance	182:205	Constructing membrane with good chlorine resistance and antifouling properties is considered to be important challenges confronting membrane applications.					
36460156	6	51	from	groups	937:942	arg1	zwitterions					966:976	azobenzene labeled zwitterions	947:976	azobenzene labeled zwitterions	947:976	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	6	52	dep	isomers	1000:1006	arg1	breaking					1080:1087	breaking	1080:1087	breaking	1080:1087	More interestingly, the trans azo groups in azobenzene labeled zwitterions can turn into the cis isomers as the visible light irradiation converted to the UV light irradiation, breaking the interaction between azobenzene labeled zwitterions and β-CD.					
36460156	1	53	theme	membrane	287:294	arg1	applications					296:307	membrane applications	287:307	membrane applications	287:307	Constructing membrane with good chlorine resistance and antifouling properties is considered to be important challenges confronting membrane applications.					
36460156	1	54	theme	good	182:185	arg1	resistance					196:205	good chlorine resistance	182:205	good chlorine resistance	182:205	Constructing membrane with good chlorine resistance and antifouling properties is considered to be important challenges confronting membrane applications.					
36460156	4	55	theme	slight	683:688	arg1	change					690:695	slight change	683:695	slight change in water flux and rejection	683:723	The optimized membrane exhibited slight change in water flux and rejection under chlorine environment.					
36460156	0	56	theme	interfacial	94:104	arg1	polymerization					106:119	interfacial polymerization	94:119	interfacial polymerization using beta cyclodextrin monomers	94:152	Preparation of chlorine-resistant and regenerable antifouling nanofiltration membrane through interfacial polymerization using beta cyclodextrin monomers.					
36460156	8	57	theme	fresh	1374:1378	arg1	solution					1411:1418	fresh azobenzene labeled zwitterions solution	1374:1418	fresh azobenzene labeled zwitterions solution	1374:1418	The antifouling membrane can be regenerated via immersing the reacquired β-CD2/10/PES membrane into fresh azobenzene labeled zwitterions solution again.					
36460156	2	58	theme	beta	429:432	arg1	monomers					454:461	beta cyclodextrin (β-CD) monomers	429:461	beta cyclodextrin (β-CD) monomers	429:461	In this study, a composite nanofiltration (NF) membrane (β-CDx/y/PES) was prepared by interfacial polymerization using beta cyclodextrin (β-CD) monomers.					
36460156	4	59	theme	chlorine	731:738	arg1	environment					740:750	chlorine environment	731:750	chlorine environment	731:750	The optimized membrane exhibited slight change in water flux and rejection under chlorine environment.					
36460156	4	60	theme	water	700:704	arg1	flux					706:709	water flux	700:709	water flux	700:709	The optimized membrane exhibited slight change in water flux and rejection under chlorine environment.					
36460156	5	61	theme	antifouling	841:851	arg1	test					853:856	the antifouling test	837:856	the antifouling test in comparison with the β-CDx/y/PES membrane	837:900	The AZ-β-CDx/y/PES membrane also displayed an evidently lower loss in water flux in the antifouling test in comparison with the β-CDx/y/PES membrane.					
36460156	7	62	theme	UV	1252:1253	arg1	irradiation					1261:1271	UV light irradiation	1252:1271	UV light irradiation	1252:1271	Hence, the contaminants upon the membrane surface can be simply eliminated by water washing under UV light irradiation.					
36460156	3	63	theme	hydrophobic	586:596	arg1	cavity					607:612	the hydrophobic internal cavity	582:612	the hydrophobic internal cavity of β-CD	582:620	Subsequently, the β-CD-based (AZ-β-CDx/y/PES) membrane was prepared by assembling azobenzene labeled zwitterions into the hydrophobic internal cavity of β-CD via host-guest interaction.					
36460156	5	64	theme	water	823:827	arg1	flux					829:832	water flux	823:832	water flux	823:832	The AZ-β-CDx/y/PES membrane also displayed an evidently lower loss in water flux in the antifouling test in comparison with the β-CDx/y/PES membrane.					
36364388	3	0	with	crosslinking	626:637	arg1	glutaraldehyde					644:657	glutaraldehyde	644:657	glutaraldehyde	644:657	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	9	1	theme	bi-functionalized	1576:1592	arg1	sorbent					1594:1600	The bi-functionalized sorbent	1572:1600	The bi-functionalized sorbent	1572:1600	The bi-functionalized sorbent was successfully tested for the recovery of cerium from pre-treated acidic leachates, recovered from low-grade cerium-bearing Egyptian ore.					
36364388	4	2	theme	elemental	824:832	arg1	analysis					834:841	elemental analysis	824:841	elemental analysis	824:841	The sorbents were characterized by FTIR, TGA, elemental analysis, SEM-EDX, TEM, VSM, and titration.					
36364388	7	3	theme	sorbent	1315:1321	arg1	selectivity					1296:1306	the selectivity	1292:1306	the selectivity of the sorbent for Ce(III)	1292:1333	The bi-functionalization strongly increases the selectivity of the sorbent for Ce(III) through multi-component equimolar solutions (especially at pH 4).					
36364388	3	4	theme	microparticles	536:549	arg1	dispersion					484:493	dispersion	484:493	dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors)	484:597	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	8	5	theme	M	1503:1503	arg1	HCl					1505:1507	0.2 M HCl	1499:1507	0.2 M HCl for the complete desorption of cerium from the loaded sorbent	1499:1569	The functionalization notably increases the stability at recycling (for at least 5 cycles), using 0.2 M HCl for the complete desorption of cerium from the loaded sorbent.					
36364388	1	6	theme	high	256:259	arg1	capacities					270:279	high sorption capacities	256:279	high sorption capacities	256:279	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	0	7	theme	Sorbent	93:99	arg1	Sulfonation-Application					101:123	Sorbent Sulfonation-Application	93:123	Sorbent Sulfonation-Application to Ore Leachate	93:139	Enhancement of Cerium Sorption onto Urea-Functionalized Magnetite Chitosan Microparticles by Sorbent Sulfonation-Application to Ore Leachate.					
36364388	5	8	theme	sorption	1006:1013	arg1	pH					910:911	pH	910:911	pH (optimum at pH 5)	910:929	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	5	8	theme	sorption	1006:1013	arg1	isotherms					1015:1023	the sorption isotherms	1002:1023	the sorption isotherms (modeled by the Langmuir equation)	1002:1058	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	7	9	theme	multi-component	1343:1357	arg1	solutions					1369:1377	multi-component equimolar solutions	1343:1377	multi-component equimolar solutions (especially at pH 4)	1343:1398	The bi-functionalization strongly increases the selectivity of the sorbent for Ce(III) through multi-component equimolar solutions (especially at pH 4).					
36364388	9	10	theme	pre-treated	1658:1668	arg1	leachates					1677:1685	pre-treated acidic leachates	1658:1685	pre-treated acidic leachates	1658:1685	The bi-functionalized sorbent was successfully tested for the recovery of cerium from pre-treated acidic leachates, recovered from low-grade cerium-bearing Egyptian ore.					
36364388	1	11	theme	rare	183:186	arg1	REEs					204:207	REEs	204:207	REEs	204:207	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	1	11	theme	rare	183:186	arg1	elements					194:201	rare earth elements	183:201	rare earth elements (REEs)	183:208	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	6	12	dep	g-1	1204:1206	arg1	mmol					1196:1199	mmol	1196:1199	mmol	1196:1199	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	6	12	dep	g-1	1204:1206	arg1	MC-UR					1213:1217	MC-UR	1213:1217	MC-UR	1213:1217	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	6	12	dep	g-1	1204:1206	arg1	MC-UR/S					1224:1230	MC-UR/S	1224:1230	MC-UR/S	1224:1230	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	6	12	dep	g-1	1204:1206	arg1	MC					1209:1210	MC	1209:1210	MC	1209:1210	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	1	13	theme	sorption	261:268	arg1	capacities					270:279	high sorption capacities	256:279	high sorption capacities	256:279	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	0	14	theme	Ore	128:130	arg1	Leachate					132:139	Ore Leachate	128:139	Ore Leachate	128:139	Enhancement of Cerium Sorption onto Urea-Functionalized Magnetite Chitosan Microparticles by Sorbent Sulfonation-Application to Ore Leachate.					
36364388	5	15	theme	pH	910:911	arg1	effect					900:905	the effect	896:905	the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation)	896:1058	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	5	16	theme	pseudo-first-order	967:984	arg1	equation					991:998	the pseudo-first-order rate equation	963:998	the pseudo-first-order rate equation	963:998	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	1	17	theme	strategic	158:166	arg1	elements					194:201	rare earth elements	183:201	rare earth elements (REEs)	183:208	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	1	17	theme	strategic	158:166	arg1	metals					168:173	strategic metals	158:173	strategic metals such as rare earth elements (REEs)	158:208	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	7	18	from	pH	1394:1395	arg1	solutions					1369:1377	multi-component equimolar solutions	1343:1377	multi-component equimolar solutions (especially at pH 4)	1343:1398	The bi-functionalization strongly increases the selectivity of the sorbent for Ce(III) through multi-component equimolar solutions (especially at pH 4).					
36364388	5	19	theme	rate	986:989	arg1	equation					991:998	the pseudo-first-order rate equation	963:998	the pseudo-first-order rate equation	963:998	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	6	20	theme	sorption	1150:1157	arg1	capacity					1159:1166	the maximum sorption capacity	1138:1166	the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively)	1138:1245	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	9	21	theme	Egyptian	1728:1735	arg1	ore					1737:1739	low-grade cerium-bearing Egyptian ore	1703:1739	low-grade cerium-bearing Egyptian ore	1703:1739	The bi-functionalized sorbent was successfully tested for the recovery of cerium from pre-treated acidic leachates, recovered from low-grade cerium-bearing Egyptian ore.					
36364388	3	22	theme	Fe	572:573	arg1	precursors					587:596	Fe(II)/Fe(III) precursors	572:596	Fe(II)/Fe(III) precursors	572:596	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	8	23	theme	complete	1517:1524	arg1	desorption					1526:1535	the complete desorption	1513:1535	the complete desorption of cerium from the loaded sorbent	1513:1569	The functionalization notably increases the stability at recycling (for at least 5 cycles), using 0.2 M HCl for the complete desorption of cerium from the loaded sorbent.					
36364388	1	24	theme	earth	188:192	arg1	REEs					204:207	REEs	204:207	REEs	204:207	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	1	24	theme	earth	188:192	arg1	elements					194:201	rare earth elements	183:201	rare earth elements (REEs)	183:208	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	0	25	theme	Magnetite	56:64	arg1	Chitosan					66:73	Urea-Functionalized Magnetite Chitosan	36:73	Urea-Functionalized Magnetite Chitosan	36:73	Enhancement of Cerium Sorption onto Urea-Functionalized Magnetite Chitosan Microparticles by Sorbent Sulfonation-Application to Ore Leachate.					
36364388	6	26	theme	maximum	1142:1148	arg1	capacity					1159:1166	the maximum sorption capacity	1138:1166	the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively)	1138:1245	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	8	27	theme	cerium	1540:1545	arg1	desorption					1526:1535	the complete desorption	1513:1535	the complete desorption of cerium from the loaded sorbent	1513:1569	The functionalization notably increases the stability at recycling (for at least 5 cycles), using 0.2 M HCl for the complete desorption of cerium from the loaded sorbent.					
36364388	3	28	theme	sulfonated	710:719	arg1	MC-UR/S					733:739	MC-UR/S	733:739	MC-UR/S	733:739	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	3	28	theme	sulfonated	710:719	arg1	derivative					721:730	its sulfonated derivative	706:730	its sulfonated derivative (MC-UR/S)	706:740	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	6	29	from	g-1	1204:1206	arg1	capacity					1159:1166	the maximum sorption capacity	1138:1166	the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively)	1138:1245	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	6	30	theme	magnetic	1110:1117	arg1	chitosan					1119:1126	magnetic chitosan	1110:1126	magnetic chitosan	1110:1126	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	3	31	theme	magnetic	449:456	arg1	chitosan					458:465	magnetic chitosan	449:465	magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde)	449:658	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	6	32	dep	0.845	1181:1185	arg1	to					1178:1179	to	1178:1179	to	1178:1179	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	5	33	theme	Langmuir	1041:1048	arg1	equation					1050:1057	the Langmuir equation	1037:1057	the Langmuir equation	1037:1057	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	3	34	theme	chitosan	458:465	arg1	properties					435:444	the sorption properties	422:444	the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde)	422:658	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	0	35	theme	Sorption	22:29	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of Cerium Sorption onto Urea-Functionalized Magnetite Chitosan	0:73	Enhancement of Cerium Sorption onto Urea-Functionalized Magnetite Chitosan Microparticles by Sorbent Sulfonation-Application to Ore Leachate.					
36364388	2	36	theme	sorbents	322:329	arg1	bi-functionality					302:317	The bi-functionality	298:317	The bi-functionality of sorbents	298:329	The bi-functionality of sorbents showed a remarkable capacity for the enhancement of binding properties.					
36364388	6	37	theme	chitosan	1119:1126	arg1	modifications					1093:1105	The successive modifications	1078:1105	The successive modifications of magnetic chitosan	1078:1126	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	0	38	theme	Cerium	15:20	arg1	Sorption					22:29	Cerium Sorption	15:29	Cerium Sorption	15:29	Enhancement of Cerium Sorption onto Urea-Functionalized Magnetite Chitosan Microparticles by Sorbent Sulfonation-Application to Ore Leachate.					
36364388	5	39	theme	second	883:888	arg1	step					890:893	a second step	881:893	a second step	881:893	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	1	40	theme	metals	168:173	arg1	recovery					146:153	The recovery	142:153	The recovery of strategic metals such as rare earth elements (REEs)	142:208	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	0	41	theme	Urea-Functionalized	36:54	arg1	Chitosan					66:73	Urea-Functionalized Magnetite Chitosan	36:73	Urea-Functionalized Magnetite Chitosan	36:73	Enhancement of Cerium Sorption onto Urea-Functionalized Magnetite Chitosan Microparticles by Sorbent Sulfonation-Application to Ore Leachate.					
36364388	8	42	from	recycling	1458:1466	arg1	stability					1445:1453	the stability	1441:1453	the stability at recycling (for at least 5 cycles)	1441:1490	The functionalization notably increases the stability at recycling (for at least 5 cycles), using 0.2 M HCl for the complete desorption of cerium from the loaded sorbent.					
36364388	5	43	theme	at	922:923	arg1	pH					925:926	optimum at pH 5	914:928	optimum at pH 5	914:928	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	5	43	theme	at	922:923	arg1	pH					910:911	pH	910:911	pH (optimum at pH 5)	910:929	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	5	44	from	pH	925:926	arg1	at					922:923	optimum at pH 5	914:928	optimum at pH 5	914:928	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	7	45	dep	pH	1394:1395	arg1	especially					1380:1389	especially	1380:1389	especially	1380:1389	The bi-functionalization strongly increases the selectivity of the sorbent for Ce(III) through multi-component equimolar solutions (especially at pH 4).					
36364388	6	46	theme	Ce	1201:1202	arg1	g-1					1204:1206	0.28 to 0.845 and 1.25 mmol Ce g-1	1173:1206	0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively)	1173:1245	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	3	47	theme	chitosan	604:611	arg1	solution					613:620	chitosan solution	604:620	chitosan solution	604:620	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	9	48	theme	acidic	1670:1675	arg1	leachates					1677:1685	pre-treated acidic leachates	1658:1685	pre-treated acidic leachates	1658:1685	The bi-functionalized sorbent was successfully tested for the recovery of cerium from pre-treated acidic leachates, recovered from low-grade cerium-bearing Egyptian ore.					
36364388	9	49	theme	cerium	1646:1651	arg1	recovery					1634:1641	the recovery	1630:1641	the recovery of cerium from pre-treated acidic leachates, recovered from low-grade cerium-bearing Egyptian ore	1630:1739	The bi-functionalized sorbent was successfully tested for the recovery of cerium from pre-treated acidic leachates, recovered from low-grade cerium-bearing Egyptian ore.					
36364388	8	50	theme	loaded	1556:1561	arg1	sorbent					1563:1569	the loaded sorbent	1552:1569	the loaded sorbent	1552:1569	The functionalization notably increases the stability at recycling (for at least 5 cycles), using 0.2 M HCl for the complete desorption of cerium from the loaded sorbent.					
36364388	6	51	dep	mmol	1196:1199	arg1	1.25					1191:1194	1.25	1191:1194	1.25	1191:1194	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	6	51	dep	mmol	1196:1199	arg1	0.845					1181:1185	0.845	1181:1185	0.845	1181:1185	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	3	52	theme	precipitated	513:524	arg1	microparticles					536:549	hydrothermally precipitated magnetite microparticles	498:549	hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors)	498:597	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	3	53	theme	sorption	426:433	arg1	properties					435:444	the sorption properties	422:444	the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde)	422:658	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	3	54	theme	urea	678:681	arg1	MC-UR					695:699	MC-UR	695:699	MC-UR	695:699	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	3	54	theme	urea	678:681	arg1	derivative					683:692	the urea derivative	674:692	the urea derivative (MC-UR)	674:700	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	3	55	theme	magnetite	526:534	arg1	microparticles					536:549	hydrothermally precipitated magnetite microparticles	498:549	hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors)	498:597	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	8	56	from	sorbent	1563:1569	arg1	desorption					1526:1535	the complete desorption	1513:1535	the complete desorption of cerium from the loaded sorbent	1513:1569	The functionalization notably increases the stability at recycling (for at least 5 cycles), using 0.2 M HCl for the complete desorption of cerium from the loaded sorbent.					
36364388	6	57	theme	successive	1082:1091	arg1	modifications					1093:1105	The successive modifications	1078:1105	The successive modifications of magnetic chitosan	1078:1126	The successive modifications of magnetic chitosan increases the maximum sorption capacity from 0.28 to 0.845 and 1.25 mmol Ce g-1 (MC, MC-UR, and MC-UR/S, respectively).					
36364388	3	58	dep	chitosan	458:465	arg1	prepared					472:479	prepared	472:479	prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde	472:657	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	3	59	theme	/Fe	578:580	arg1	precursors					587:596	Fe(II)/Fe(III) precursors	572:596	Fe(II)/Fe(III) precursors	572:596	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	5	60	theme	uptake	936:941	arg1	kinetics					943:950	the uptake kinetics	932:950	the uptake kinetics (fitted by the pseudo-first-order rate equation)	932:999	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	5	60	theme	uptake	936:941	arg1	pH					910:911	pH	910:911	pH (optimum at pH 5)	910:929	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	3	61	dep	prepared	472:479	arg1	MC					468:469	MC	468:469	MC	468:469	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	1	62	with	sorbents	242:249	arg1	selectivity					285:295	selectivity	285:295	selectivity	285:295	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	1	62	with	sorbents	242:249	arg1	capacities					270:279	high sorption capacities	256:279	high sorption capacities	256:279	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	2	63	theme	remarkable	340:349	arg1	capacity					351:358	a remarkable capacity	338:358	a remarkable capacity for the enhancement of binding properties	338:400	The bi-functionality of sorbents showed a remarkable capacity for the enhancement of binding properties.					
36364388	8	64	theme	0.2	1499:1501	arg1	M					1503:1503	M	1503:1503	M	1503:1503	The functionalization notably increases the stability at recycling (for at least 5 cycles), using 0.2 M HCl for the complete desorption of cerium from the loaded sorbent.					
36364388	1	65	theme	new	238:240	arg1	sorbents					242:249	new sorbents	238:249	new sorbents with high sorption capacities and selectivity	238:295	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	3	66	with	solution	613:620	arg1	glutaraldehyde					644:657	glutaraldehyde	644:657	glutaraldehyde	644:657	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36364388	7	67	theme	equimolar	1359:1367	arg1	solutions					1369:1377	multi-component equimolar solutions	1343:1377	multi-component equimolar solutions (especially at pH 4)	1343:1398	The bi-functionalization strongly increases the selectivity of the sorbent for Ce(III) through multi-component equimolar solutions (especially at pH 4).					
36364388	9	68	from	leachates	1677:1685	arg1	recovery					1634:1641	the recovery	1630:1641	the recovery of cerium from pre-treated acidic leachates, recovered from low-grade cerium-bearing Egyptian ore	1630:1739	The bi-functionalized sorbent was successfully tested for the recovery of cerium from pre-treated acidic leachates, recovered from low-grade cerium-bearing Egyptian ore.					
36364388	2	69	theme	properties	391:400	arg1	enhancement					368:378	the enhancement	364:378	the enhancement of binding properties	364:400	The bi-functionality of sorbents showed a remarkable capacity for the enhancement of binding properties.					
36364388	9	70	theme	low-grade	1703:1711	arg1	ore					1737:1739	low-grade cerium-bearing Egyptian ore	1703:1739	low-grade cerium-bearing Egyptian ore	1703:1739	The bi-functionalized sorbent was successfully tested for the recovery of cerium from pre-treated acidic leachates, recovered from low-grade cerium-bearing Egyptian ore.					
36364388	1	71	theme	sorbents	242:249	arg1	development					223:233	the development	219:233	the development of new sorbents with high sorption capacities and selectivity	219:295	The recovery of strategic metals such as rare earth elements (REEs) requires the development of new sorbents with high sorption capacities and selectivity.					
36364388	5	72	theme	optimum	914:920	arg1	pH					925:926	optimum at pH 5	914:928	optimum at pH 5	914:928	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	5	72	theme	optimum	914:920	arg1	pH					910:911	pH	910:911	pH (optimum at pH 5)	910:929	In a second step, the effect of pH (optimum at pH 5), the uptake kinetics (fitted by the pseudo-first-order rate equation), the sorption isotherms (modeled by the Langmuir equation) are investigated.					
36364388	2	73	theme	binding	383:389	arg1	properties					391:400	binding properties	383:400	binding properties	383:400	The bi-functionality of sorbents showed a remarkable capacity for the enhancement of binding properties.					
36364388	9	74	theme	cerium-bearing	1713:1726	arg1	ore					1737:1739	low-grade cerium-bearing Egyptian ore	1703:1739	low-grade cerium-bearing Egyptian ore	1703:1739	The bi-functionalized sorbent was successfully tested for the recovery of cerium from pre-treated acidic leachates, recovered from low-grade cerium-bearing Egyptian ore.					
36364388	3	75	theme	REEs	771:774	arg1	example					760:766	an example	757:766	an example of REEs	757:774	This work compares the sorption properties of magnetic chitosan (MC, prepared by dispersion of hydrothermally precipitated magnetite microparticles (synthesized through Fe(II)/Fe(III) precursors) into chitosan solution and crosslinking with glutaraldehyde) with those of the urea derivative (MC-UR) and its sulfonated derivative (MC-UR/S) for cerium (as an example of REEs).					
36055416	6	0	dep	CPIR	873:876	arg1	relative					879:886	relative	879:886	relative	879:886	Mice on the Gluc diets exhibited higher CPIR (relative to baseline) by the end of exposure period 1, whereas mice on the control and HiFruc diets did not do so until the end of exposure period 2.					
36055416	9	1	theme	glucose	1387:1393	arg1	tolerance					1395:1403	glucose tolerance	1387:1403	glucose tolerance	1387:1403	We found, once again, that consumption of the Gluc (but not the HiFruc) diet enhanced glucose tolerance, in part by increasing CPIR and insulin sensitivity.					
36055416	2	2	from	tolerance	261:269	arg1	mice					274:277	mice	274:277	mice	274:277	Here, we tested the hypothesis that ad lib consumption of glucose (Gluc) or high-fructose (HiFruc) syrups improves glucose tolerance in mice.					
36055416	5	3	theme	diets	818:822	arg1	consumption					792:802	consumption	792:802	consumption of the HiFruc diets	792:822	In Experiment 1, consumption of the Gluc diets reliably enhanced glucose tolerance, while consumption of the HiFruc diets did not.					
36055416	8	4	theme	corn-based	1266:1275	arg1	Gluc					1277:1280	the corn-based Gluc	1262:1280	the corn-based Gluc	1262:1280	In Experiment 2, we repeated the previous experiment, but limited testing to the corn-based Gluc and HiFruc syrups.					
36055416	6	5	theme	control	954:960	arg1	diets					973:977	the control and HiFruc diets	950:977	diets	973:977	Mice on the Gluc diets exhibited higher CPIR (relative to baseline) by the end of exposure period 1, whereas mice on the control and HiFruc diets did not do so until the end of exposure period 2.					
36055416	4	6	theme	Gluc	616:619	arg1	concentrations					598:611	11% concentrations	594:611	11% concentrations of Gluc or HiFruc syrup	594:635	The sugar solutions contained 11% concentrations of Gluc or HiFruc syrup; these syrups were derived from either corn starch or cellulose.					
36055416	8	7	theme	HiFruc	1286:1291	arg1	syrups					1293:1298	HiFruc syrups	1286:1298	HiFruc syrups	1286:1298	In Experiment 2, we repeated the previous experiment, but limited testing to the corn-based Gluc and HiFruc syrups.					
36055416	3	8	theme	control	312:318	arg1	chow					321:324	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	chow	321:324	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	9	theme	insulin	543:549	arg1	sensitivity					551:561	insulin sensitivity	543:561	insulin sensitivity	543:561	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	2	10	theme	glucose	253:259	arg1	tolerance					261:269	glucose tolerance	253:269	glucose tolerance in mice	253:277	Here, we tested the hypothesis that ad lib consumption of glucose (Gluc) or high-fructose (HiFruc) syrups improves glucose tolerance in mice.					
36055416	3	11	theme	glucose	482:488	arg1	tolerance					490:498	glucose tolerance	482:498	glucose tolerance	482:498	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	9	12	theme	Gluc	1347:1350	arg1	diet					1373:1376	the Gluc (but not the HiFruc) diet	1343:1376	the Gluc (but not the HiFruc) diet	1343:1376	We found, once again, that consumption of the Gluc (but not the HiFruc) diet enhanced glucose tolerance, in part by increasing CPIR and insulin sensitivity.					
36055416	9	12	theme	Gluc	1347:1350	arg1	HiFruc					1365:1370	the HiFruc	1361:1370	the HiFruc	1361:1370	We found, once again, that consumption of the Gluc (but not the HiFruc) diet enhanced glucose tolerance, in part by increasing CPIR and insulin sensitivity.					
36055416	6	13	theme	exposure	1010:1017	arg1	period					1019:1024	exposure period 2	1010:1026	exposure period 2	1010:1026	Mice on the Gluc diets exhibited higher CPIR (relative to baseline) by the end of exposure period 1, whereas mice on the control and HiFruc diets did not do so until the end of exposure period 2.					
36055416	6	14	from	mice	942:945	arg1	diets					973:977	the control and HiFruc diets	950:977	diets	973:977	Mice on the Gluc diets exhibited higher CPIR (relative to baseline) by the end of exposure period 1, whereas mice on the control and HiFruc diets did not do so until the end of exposure period 2.					
36055416	4	15	dep	Gluc	616:619	arg1	syrup					631:635	syrup	631:635	syrup	631:635	The sugar solutions contained 11% concentrations of Gluc or HiFruc syrup; these syrups were derived from either corn starch or cellulose.					
36055416	7	16	from	mice	1151:1154	arg1	diets					1170:1174	the HiFruc diets	1159:1174	the HiFruc diets	1159:1174	Mice on the Gluc diets also exhibited higher insulin sensitivity than control mice at the end of exposure period 2, while mice on the HiFruc diets did not.					
36055416	3	17	theme	consecutive	402:412	arg1	periods					430:436	two consecutive 28-day exposure periods	398:436	two consecutive 28-day exposure periods	398:436	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	5	18	theme	diets	743:747	arg1	consumption					719:729	consumption	719:729	consumption of the Gluc diets	719:747	In Experiment 1, consumption of the Gluc diets reliably enhanced glucose tolerance, while consumption of the HiFruc diets did not.					
36055416	0	19	from	tolerance	46:54	arg1	mice					59:62	mice	59:62	mice	59:62	Consumption of glucose syrup enhances glucose tolerance in mice.					
36055416	5	20	theme	Gluc	738:741	arg1	diets					743:747	the Gluc diets	734:747	the Gluc diets	734:747	In Experiment 1, consumption of the Gluc diets reliably enhanced glucose tolerance, while consumption of the HiFruc diets did not.					
36055416	5	21	theme	HiFruc	811:816	arg1	diets					818:822	the HiFruc diets	807:822	the HiFruc diets	807:822	In Experiment 1, consumption of the Gluc diets reliably enhanced glucose tolerance, while consumption of the HiFruc diets did not.					
36055416	6	22	theme	exposure	915:922	arg1	period					924:929	exposure period 1	915:931	exposure period 1	915:931	Mice on the Gluc diets exhibited higher CPIR (relative to baseline) by the end of exposure period 1, whereas mice on the control and HiFruc diets did not do so until the end of exposure period 2.					
36055416	3	23	with	changes	453:459	arg1	water					330:334	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	water	330:334	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	23	with	changes	453:459	arg1	chow					321:324	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	chow	321:324	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	23	with	changes	453:459	arg1	diet					386:389	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	diet	386:389	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	23	with	changes	453:459	arg1	water					360:364	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	water	360:364	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	7	24	theme	exposure	1126:1133	arg1	period					1135:1140	exposure period 2	1126:1142	exposure period 2	1126:1142	Mice on the Gluc diets also exhibited higher insulin sensitivity than control mice at the end of exposure period 2, while mice on the HiFruc diets did not.					
36055416	7	25	theme	HiFruc	1163:1168	arg1	diets					1170:1174	the HiFruc diets	1159:1174	the HiFruc diets	1159:1174	Mice on the Gluc diets also exhibited higher insulin sensitivity than control mice at the end of exposure period 2, while mice on the HiFruc diets did not.					
36055416	3	26	theme	sugar	370:374	arg1	diet					386:389	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	diet	386:389	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	6	27	theme	Gluc	845:848	arg1	diets					850:854	the Gluc diets	841:854	the Gluc diets	841:854	Mice on the Gluc diets exhibited higher CPIR (relative to baseline) by the end of exposure period 1, whereas mice on the control and HiFruc diets did not do so until the end of exposure period 2.					
36055416	7	28	theme	Gluc	1041:1044	arg1	diets					1046:1050	the Gluc diets	1037:1050	the Gluc diets	1037:1050	Mice on the Gluc diets also exhibited higher insulin sensitivity than control mice at the end of exposure period 2, while mice on the HiFruc diets did not.					
36055416	10	29	theme	glucose	1519:1525	arg1	diets					1527:1531	high glucose diets	1514:1531	high glucose diets	1514:1531	These results show that mice can adapt metabolically to high glucose diets, and that this adaptation process involves upregulating at least two components of the insulin response system.					
36055416	7	30	theme	control	1099:1105	arg1	mice					1107:1110	control mice	1099:1110	control mice	1099:1110	Mice on the Gluc diets also exhibited higher insulin sensitivity than control mice at the end of exposure period 2, while mice on the HiFruc diets did not.					
36055416	4	31	theme	%	596:596	arg1	concentrations					598:611	11% concentrations	594:611	11% concentrations of Gluc or HiFruc syrup	594:635	The sugar solutions contained 11% concentrations of Gluc or HiFruc syrup; these syrups were derived from either corn starch or cellulose.					
36055416	6	32	theme	higher	866:871	arg1	CPIR					873:876	higher CPIR	866:876	higher CPIR (relative to baseline)	866:899	Mice on the Gluc diets exhibited higher CPIR (relative to baseline) by the end of exposure period 1, whereas mice on the control and HiFruc diets did not do so until the end of exposure period 2.					
36055416	3	33	from	mice	300:303	arg1	release					524:530	cephalic-phase insulin release	501:530	cephalic-phase insulin release (CPIR)	501:537	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	33	from	mice	300:303	arg1	sensitivity					551:561	insulin sensitivity	543:561	insulin sensitivity	543:561	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	33	from	mice	300:303	arg1	composition					469:479	body composition	464:479	body composition	464:479	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	33	from	mice	300:303	arg1	tolerance					490:498	glucose tolerance	482:498	glucose tolerance	482:498	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	33	from	mice	300:303	arg1	CPIR					533:536	CPIR	533:536	CPIR	533:536	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	5	34	theme	glucose	767:773	arg1	tolerance					775:783	glucose tolerance	767:783	glucose tolerance	767:783	In Experiment 1, consumption of the Gluc diets reliably enhanced glucose tolerance, while consumption of the HiFruc diets did not.					
36055416	4	35	theme	corn	676:679	arg1	starch					681:686	corn starch	676:686	corn starch	676:686	The sugar solutions contained 11% concentrations of Gluc or HiFruc syrup; these syrups were derived from either corn starch or cellulose.					
36055416	4	36	theme	11	594:595	arg1	%					596:596	%	596:596	%	596:596	The sugar solutions contained 11% concentrations of Gluc or HiFruc syrup; these syrups were derived from either corn starch or cellulose.					
36055416	0	37	theme	syrup	23:27	arg1	Consumption					0:10	Consumption	0:10	Consumption of glucose syrup	0:27	Consumption of glucose syrup enhances glucose tolerance in mice.					
36055416	8	38	theme	previous	1218:1225	arg1	experiment					1227:1236	the previous experiment	1214:1236	the previous experiment	1214:1236	In Experiment 2, we repeated the previous experiment, but limited testing to the corn-based Gluc and HiFruc syrups.					
36055416	2	39	theme	glucose	196:202	arg1	consumption					181:191	ad lib consumption	174:191	ad lib consumption of glucose (Gluc) or high-fructose (HiFruc) syrups	174:242	Here, we tested the hypothesis that ad lib consumption of glucose (Gluc) or high-fructose (HiFruc) syrups improves glucose tolerance in mice.					
36055416	10	40	theme	high	1514:1517	arg1	diets					1527:1531	high glucose diets	1514:1531	high glucose diets	1514:1531	These results show that mice can adapt metabolically to high glucose diets, and that this adaptation process involves upregulating at least two components of the insulin response system.					
36055416	6	41	theme	period	924:929	arg1	end					908:910	the end	904:910	the end of exposure period 1	904:931	Mice on the Gluc diets exhibited higher CPIR (relative to baseline) by the end of exposure period 1, whereas mice on the control and HiFruc diets did not do so until the end of exposure period 2.					
36055416	0	42	theme	glucose	15:21	arg1	syrup					23:27	glucose syrup	15:27	glucose syrup	15:27	Consumption of glucose syrup enhances glucose tolerance in mice.					
36055416	9	43	dep	HiFruc	1365:1370	arg1	not					1357:1359	not	1357:1359	not	1357:1359	We found, once again, that consumption of the Gluc (but not the HiFruc) diet enhanced glucose tolerance, in part by increasing CPIR and insulin sensitivity.					
36055416	9	44	theme	insulin	1437:1443	arg1	sensitivity					1445:1455	insulin sensitivity	1437:1455	insulin sensitivity	1437:1455	We found, once again, that consumption of the Gluc (but not the HiFruc) diet enhanced glucose tolerance, in part by increasing CPIR and insulin sensitivity.					
36055416	6	45	theme	period	1019:1024	arg1	end					1003:1005	the end	999:1005	the end of exposure period 2	999:1026	Mice on the Gluc diets exhibited higher CPIR (relative to baseline) by the end of exposure period 1, whereas mice on the control and HiFruc diets did not do so until the end of exposure period 2.					
36055416	1	46	theme	metabolic	91:99	arg1	impact					101:106	the metabolic impact	87:106	the metabolic impact of sugar-sweetened beverages	87:135	There is debate about the metabolic impact of sugar-sweetened beverages.					
36055416	2	47	dep	glucose	196:202	arg1	syrups					237:242	syrups	237:242	syrups	237:242	Here, we tested the hypothesis that ad lib consumption of glucose (Gluc) or high-fructose (HiFruc) syrups improves glucose tolerance in mice.					
36055416	0	48	theme	glucose	38:44	arg1	tolerance					46:54	glucose tolerance	38:54	glucose tolerance in mice	38:62	Consumption of glucose syrup enhances glucose tolerance in mice.					
36055416	4	49	theme	HiFruc	624:629	arg1	concentrations					598:611	11% concentrations	594:611	11% concentrations of Gluc or HiFruc syrup	594:635	The sugar solutions contained 11% concentrations of Gluc or HiFruc syrup; these syrups were derived from either corn starch or cellulose.					
36055416	4	50	theme	sugar	568:572	arg1	solutions					574:582	The sugar solutions	564:582	The sugar solutions	564:582	The sugar solutions contained 11% concentrations of Gluc or HiFruc syrup; these syrups were derived from either corn starch or cellulose.					
36055416	3	51	theme	solution	376:383	arg1	diet					386:389	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	diet	386:389	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	52	theme	chow	354:357	arg1	water					360:364	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	water	360:364	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	7	53	from	Mice	1029:1032	arg1	diets					1046:1050	the Gluc diets	1037:1050	the Gluc diets	1037:1050	Mice on the Gluc diets also exhibited higher insulin sensitivity than control mice at the end of exposure period 2, while mice on the HiFruc diets did not.					
36055416	9	54	theme	diet	1373:1376	arg1	consumption					1328:1338	consumption	1328:1338	consumption of the Gluc (but not the HiFruc) diet	1328:1376	We found, once again, that consumption of the Gluc (but not the HiFruc) diet enhanced glucose tolerance, in part by increasing CPIR and insulin sensitivity.					
36055416	7	55	theme	higher	1067:1072	arg1	sensitivity					1082:1092	higher insulin sensitivity	1067:1092	higher insulin sensitivity	1067:1092	Mice on the Gluc diets also exhibited higher insulin sensitivity than control mice at the end of exposure period 2, while mice on the HiFruc diets did not.					
36055416	6	56	from	Mice	833:836	arg1	diets					850:854	the Gluc diets	841:854	the Gluc diets	841:854	Mice on the Gluc diets exhibited higher CPIR (relative to baseline) by the end of exposure period 1, whereas mice on the control and HiFruc diets did not do so until the end of exposure period 2.					
36055416	3	57	theme	cephalic-phase	501:514	arg1	release					524:530	cephalic-phase insulin release	501:530	cephalic-phase insulin release (CPIR)	501:537	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	57	theme	cephalic-phase	501:514	arg1	CPIR					533:536	CPIR	533:536	CPIR	533:536	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	58	theme	28-day	414:419	arg1	periods					430:436	two consecutive 28-day exposure periods	398:436	two consecutive 28-day exposure periods	398:436	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	59	theme	experimental	340:351	arg1	water					360:364	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	water	360:364	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	4	60	attach	derived	656:662	arg2	syrups					644:649	these syrups	638:649	these syrups	638:649	The sugar solutions contained 11% concentrations of Gluc or HiFruc syrup; these syrups were derived from either corn starch or cellulose.					
36055416	4	60	attach	derived	656:662	arg1	cellulose					691:699	cellulose	691:699	cellulose	691:699	The sugar solutions contained 11% concentrations of Gluc or HiFruc syrup; these syrups were derived from either corn starch or cellulose.					
36055416	4	60	attach	derived	656:662	arg1	starch					681:686	corn starch	676:686	corn starch	676:686	The sugar solutions contained 11% concentrations of Gluc or HiFruc syrup; these syrups were derived from either corn starch or cellulose.					
36055416	10	61	theme	response	1628:1635	arg1	system					1637:1642	the insulin response system	1616:1642	the insulin response system	1616:1642	These results show that mice can adapt metabolically to high glucose diets, and that this adaptation process involves upregulating at least two components of the insulin response system.					
36055416	3	62	theme	insulin	516:522	arg1	release					524:530	cephalic-phase insulin release	501:530	cephalic-phase insulin release (CPIR)	501:537	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	62	theme	insulin	516:522	arg1	CPIR					533:536	CPIR	533:536	CPIR	533:536	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	10	63	theme	system	1637:1642	arg1	components					1602:1611	at least two components	1589:1611	at least two components of the insulin response system	1589:1642	These results show that mice can adapt metabolically to high glucose diets, and that this adaptation process involves upregulating at least two components of the insulin response system.					
36055416	3	64	theme	exposure	421:428	arg1	periods					430:436	two consecutive 28-day exposure periods	398:436	two consecutive 28-day exposure periods	398:436	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	10	65	theme	adaptation	1548:1557	arg1	process					1559:1565	this adaptation process	1543:1565	this adaptation process	1543:1565	These results show that mice can adapt metabolically to high glucose diets, and that this adaptation process involves upregulating at least two components of the insulin response system.					
36055416	10	66	theme	insulin	1620:1626	arg1	system					1637:1642	the insulin response system	1616:1642	the insulin response system	1616:1642	These results show that mice can adapt metabolically to high glucose diets, and that this adaptation process involves upregulating at least two components of the insulin response system.					
36055416	1	67	theme	sugar-sweetened	111:125	arg1	beverages					127:135	sugar-sweetened beverages	111:135	sugar-sweetened beverages	111:135	There is debate about the metabolic impact of sugar-sweetened beverages.					
36055416	6	68	theme	HiFruc	966:971	arg1	diets					973:977	the control and HiFruc diets	950:977	diets	973:977	Mice on the Gluc diets exhibited higher CPIR (relative to baseline) by the end of exposure period 1, whereas mice on the control and HiFruc diets did not do so until the end of exposure period 2.					
36055416	2	69	theme	lib	177:179	arg1	consumption					181:191	ad lib consumption	174:191	ad lib consumption of glucose (Gluc) or high-fructose (HiFruc) syrups	174:242	Here, we tested the hypothesis that ad lib consumption of glucose (Gluc) or high-fructose (HiFruc) syrups improves glucose tolerance in mice.					
36055416	3	70	theme	body	464:467	arg1	composition					469:479	body composition	464:479	body composition	464:479	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	1	71	theme	beverages	127:135	arg1	impact					101:106	the metabolic impact	87:106	the metabolic impact of sugar-sweetened beverages	87:135	There is debate about the metabolic impact of sugar-sweetened beverages.					
36055416	7	72	theme	period	1135:1140	arg1	end					1119:1121	the end	1115:1121	the end of exposure period 2	1115:1142	Mice on the Gluc diets also exhibited higher insulin sensitivity than control mice at the end of exposure period 2, while mice on the HiFruc diets did not.					
36055416	3	73	theme	C57BL/6	292:298	arg1	mice					300:303	C57BL/6 mice	292:303	C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods	292:436	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	2	74	theme	ad	174:175	arg1	consumption					181:191	ad lib consumption	174:191	ad lib consumption of glucose (Gluc) or high-fructose (HiFruc) syrups	174:242	Here, we tested the hypothesis that ad lib consumption of glucose (Gluc) or high-fructose (HiFruc) syrups improves glucose tolerance in mice.					
36055416	2	75	theme	high-fructose	214:226	arg1	consumption					181:191	ad lib consumption	174:191	ad lib consumption of glucose (Gluc) or high-fructose (HiFruc) syrups	174:242	Here, we tested the hypothesis that ad lib consumption of glucose (Gluc) or high-fructose (HiFruc) syrups improves glucose tolerance in mice.					
36055416	7	76	theme	insulin	1074:1080	arg1	sensitivity					1082:1092	higher insulin sensitivity	1067:1092	higher insulin sensitivity	1067:1092	Mice on the Gluc diets also exhibited higher insulin sensitivity than control mice at the end of exposure period 2, while mice on the HiFruc diets did not.					
36055416	4	77	contain	contained	584:592	arg2	concentrations					598:611	11% concentrations	594:611	11% concentrations of Gluc or HiFruc syrup	594:635	The sugar solutions contained 11% concentrations of Gluc or HiFruc syrup; these syrups were derived from either corn starch or cellulose.					
36055416	4	77	contain	contained	584:592	arg1	solutions					574:582	The sugar solutions	564:582	The sugar solutions	564:582	The sugar solutions contained 11% concentrations of Gluc or HiFruc syrup; these syrups were derived from either corn starch or cellulose.					
36055416	3	78	with	mice	300:303	arg1	water					330:334	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	water	330:334	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	78	with	mice	300:303	arg1	chow					321:324	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	chow	321:324	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	78	with	mice	300:303	arg1	diet					386:389	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	diet	386:389	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	78	with	mice	300:303	arg1	water					360:364	a control (chow and water) or experimental (chow, water and sugar solution) diet	310:389	water	360:364	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	79	theme	monitored	443:451	arg1	changes					453:459	monitored changes	443:459	monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity	443:561	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	80	from	changes	453:459	arg1	release					524:530	cephalic-phase insulin release	501:530	cephalic-phase insulin release (CPIR)	501:537	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	80	from	changes	453:459	arg1	sensitivity					551:561	insulin sensitivity	543:561	insulin sensitivity	543:561	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	80	from	changes	453:459	arg1	composition					469:479	body composition	464:479	body composition	464:479	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	80	from	changes	453:459	arg1	tolerance					490:498	glucose tolerance	482:498	glucose tolerance	482:498	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
36055416	3	80	from	changes	453:459	arg1	CPIR					533:536	CPIR	533:536	CPIR	533:536	We provided C57BL/6 mice with a control (chow and water) or experimental (chow, water and sugar solution) diet across two consecutive 28-day exposure periods, and monitored changes in body composition, glucose tolerance, cephalic-phase insulin release (CPIR) and insulin sensitivity.					
37224897	10	0	theme	effective	1526:1534	arg1	way					1536:1538	an effective way	1523:1538	an effective way to improve tomato quality and maintain soil nutrients during tomato ripening	1523:1615	Therefore, kaolinite clay, as part of the coating system, can be an effective way to improve tomato quality and maintain soil nutrients during tomato ripening.					
37224897	10	0	theme	effective	1526:1534	arg1	clay					1479:1482	kaolinite clay	1469:1482	kaolinite clay	1469:1482	Therefore, kaolinite clay, as part of the coating system, can be an effective way to improve tomato quality and maintain soil nutrients during tomato ripening.					
37224897	10	1	theme	system	1508:1513	arg1	part					1488:1491	part	1488:1491	part of the coating system	1488:1513	Therefore, kaolinite clay, as part of the coating system, can be an effective way to improve tomato quality and maintain soil nutrients during tomato ripening.					
37224897	5	2	theme	fertilizer	880:889	arg1	release					858:864	the sustained nutrient release	835:864	the sustained nutrient release of coated NPK fertilizer (NPK/CsGC)	835:900	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	4	3	theme	coating	763:769	arg1	composite					671:679	Chitosan composite	662:679	Chitosan composite (CsGC)	662:686	For this purpose, Chitosan composite (CsGC) was adopted as a durable coating material, including clay as a reinforcing coating material.					
37224897	4	3	theme	coating	763:769	arg1	material					771:778	a reinforcing coating material	749:778	a reinforcing coating material	749:778	For this purpose, Chitosan composite (CsGC) was adopted as a durable coating material, including clay as a reinforcing coating material.					
37224897	0	4	from	growth	174:179	arg1	nutrients					153:161	soil nutrients	148:161	soil nutrients	148:161	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	0	5	theme	release	113:119	arg1	growth					174:179	tomato growth	167:179	tomato growth	167:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	0	5	theme	release	113:119	arg1	Effect					138:143	Effect	138:143	Effect on soil nutrients	138:161	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	0	5	theme	release	113:119	arg1	fertilizers					125:135	slow release NPK fertilizers	108:135	slow release NPK fertilizers: Effect on soil nutrients and tomato growth	108:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	0	6	dep	fertilizers	125:135	arg1	fertilizers					125:135	slow release NPK fertilizers	108:135	slow release NPK fertilizers: Effect on soil nutrients and tomato growth	108:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	0	6	dep	fertilizers	125:135	arg1	Effect					138:143	Effect	138:143	Effect on soil nutrients	138:161	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	0	6	dep	fertilizers	125:135	arg1	growth					174:179	tomato growth	167:179	tomato growth	167:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	10	7	theme	tomato	1601:1606	arg1	ripening					1608:1615	tomato ripening	1601:1615	tomato ripening	1601:1615	Therefore, kaolinite clay, as part of the coating system, can be an effective way to improve tomato quality and maintain soil nutrients during tomato ripening.					
37224897	9	8	theme	representative	1427:1440	arg1	nutrients					1447:1455	representative soil nutrients	1427:1455	representative soil nutrients	1427:1455	Furthermore, the surface response study confirmed a strong correlation between tomato quality and representative soil nutrients.					
37224897	0	9	theme	slow	108:111	arg1	growth					174:179	tomato growth	167:179	tomato growth	167:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	0	9	theme	slow	108:111	arg1	Effect					138:143	Effect	138:143	Effect on soil nutrients	138:161	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	0	9	theme	slow	108:111	arg1	fertilizers					125:135	slow release NPK fertilizers	108:135	slow release NPK fertilizers: Effect on soil nutrients and tomato growth	108:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	7	10	theme	mechanical	1102:1111	arg1	strength					1113:1120	the mechanical strength	1098:1120	the mechanical strength of the NPK fertilizer	1098:1142	Results revealed that the proposed coating film improved the mechanical strength of the NPK fertilizer and enhanced water retention capacity of the soil.					
37224897	6	11	theme	Scanning	915:922	arg1	microscopy					933:942	Scanning electron microscopy	915:942	Scanning electron microscopy	915:942	Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were used to examine the coated NPK granules.					
37224897	3	12	theme	material	554:561	arg1	effect					528:533	the effect	524:533	the effect of a durable liner material based on biodegradable polymers on soil nutrient availability and tomato growth	524:641	This work aims to assess the effect of a durable liner material based on biodegradable polymers on soil nutrient availability and tomato growth.					
37224897	9	13	theme	soil	1442:1445	arg1	nutrients					1447:1455	representative soil nutrients	1427:1455	representative soil nutrients	1427:1455	Furthermore, the surface response study confirmed a strong correlation between tomato quality and representative soil nutrients.					
37224897	10	14	theme	tomato	1551:1556	arg1	quality					1558:1564	tomato quality	1551:1564	tomato quality	1551:1564	Therefore, kaolinite clay, as part of the coating system, can be an effective way to improve tomato quality and maintain soil nutrients during tomato ripening.					
37224897	4	15	theme	durable	705:711	arg1	composite					671:679	Chitosan composite	662:679	Chitosan composite (CsGC)	662:686	For this purpose, Chitosan composite (CsGC) was adopted as a durable coating material, including clay as a reinforcing coating material.					
37224897	4	15	theme	durable	705:711	arg1	material					721:728	a durable coating material	703:728	a durable coating material	703:728	For this purpose, Chitosan composite (CsGC) was adopted as a durable coating material, including clay as a reinforcing coating material.					
37224897	6	16	used	used	1000:1003	arg2	spectroscopy					972:983	energy-dispersive X-ray spectroscopy	948:983	energy-dispersive X-ray spectroscopy (SEM/EDX)	948:993	Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were used to examine the coated NPK granules.					
37224897	6	16	used	used	1000:1003	arg2	microscopy					933:942	Scanning electron microscopy	915:942	Scanning electron microscopy	915:942	Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were used to examine the coated NPK granules.					
37224897	6	16	used	used	1000:1003	arg2	SEM/EDX					986:992	SEM/EDX	986:992	SEM/EDX	986:992	Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were used to examine the coated NPK granules.					
37224897	2	17	theme	adverse	408:414	arg1	effects					416:422	obviating adverse effects	398:422	obviating adverse effects of over-fertilization on soil nutrients and, consequently, on crop yields	398:496	Proper fertilization practices can lead to obviating adverse effects of over-fertilization on soil nutrients and, consequently, on crop yields.					
37224897	5	18	theme	sustained	839:847	arg1	release					858:864	the sustained nutrient release	835:864	the sustained nutrient release of coated NPK fertilizer (NPK/CsGC)	835:900	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	0	19	theme	NPK	121:123	arg1	growth					174:179	tomato growth	167:179	tomato growth	167:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	0	19	theme	NPK	121:123	arg1	Effect					138:143	Effect	138:143	Effect on soil nutrients	138:161	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	0	19	theme	NPK	121:123	arg1	fertilizers					125:135	slow release NPK fertilizers	108:135	slow release NPK fertilizers: Effect on soil nutrients and tomato growth	108:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	8	20	theme	tomato	1310:1315	arg1	metabolism					1317:1326	tomato metabolism	1310:1326	tomato metabolism	1310:1326	The agronomic investigation has also proved their outstanding potential to boost chlorophyll content, biomass, and tomato metabolism.					
37224897	7	21	theme	fertilizer	1133:1142	arg1	strength					1113:1120	the mechanical strength	1098:1120	the mechanical strength of the NPK fertilizer	1098:1142	Results revealed that the proposed coating film improved the mechanical strength of the NPK fertilizer and enhanced water retention capacity of the soil.					
37224897	7	22	theme	soil	1189:1192	arg1	capacity					1173:1180	water retention capacity	1157:1180	water retention capacity of the soil	1157:1192	Results revealed that the proposed coating film improved the mechanical strength of the NPK fertilizer and enhanced water retention capacity of the soil.					
37224897	3	23	theme	durable	540:546	arg1	material					554:561	a durable liner material	538:561	a durable liner material	538:561	This work aims to assess the effect of a durable liner material based on biodegradable polymers on soil nutrient availability and tomato growth.					
37224897	1	24	theme	Soil	182:185	arg1	availability					196:207	Soil nutrient availability	182:207	Soil nutrient availability	182:207	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	5	25	theme	chitosan	798:805	arg1	CsGC					826:829	CsGC	826:829	CsGC	826:829	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	5	25	theme	chitosan	798:805	arg1	coating					817:823	chitosan composite coating	798:823	chitosan composite coating (CsGC)	798:830	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	9	26	theme	surface	1346:1352	arg1	study					1363:1367	the surface response study	1342:1367	the surface response study	1342:1367	Furthermore, the surface response study confirmed a strong correlation between tomato quality and representative soil nutrients.					
37224897	7	27	theme	proposed	1067:1074	arg1	film					1084:1087	the proposed coating film	1063:1087	the proposed coating film	1063:1087	Results revealed that the proposed coating film improved the mechanical strength of the NPK fertilizer and enhanced water retention capacity of the soil.					
37224897	4	28	theme	Chitosan	662:669	arg1	composite					671:679	Chitosan composite	662:679	Chitosan composite (CsGC)	662:686	For this purpose, Chitosan composite (CsGC) was adopted as a durable coating material, including clay as a reinforcing coating material.					
37224897	4	28	theme	Chitosan	662:669	arg1	material					771:778	a reinforcing coating material	749:778	a reinforcing coating material	749:778	For this purpose, Chitosan composite (CsGC) was adopted as a durable coating material, including clay as a reinforcing coating material.					
37224897	4	28	theme	Chitosan	662:669	arg1	CsGC					682:685	CsGC	682:685	CsGC	682:685	For this purpose, Chitosan composite (CsGC) was adopted as a durable coating material, including clay as a reinforcing coating material.					
37224897	4	28	theme	Chitosan	662:669	arg1	material					721:728	a durable coating material	703:728	a durable coating material	703:728	For this purpose, Chitosan composite (CsGC) was adopted as a durable coating material, including clay as a reinforcing coating material.					
37224897	3	29	theme	biodegradable	572:584	arg1	polymers					586:593	biodegradable polymers	572:593	biodegradable polymers on soil nutrient availability and tomato growth	572:641	This work aims to assess the effect of a durable liner material based on biodegradable polymers on soil nutrient availability and tomato growth.					
37224897	7	30	theme	retention	1163:1171	arg1	capacity					1173:1180	water retention capacity	1157:1180	water retention capacity of the soil	1157:1192	Results revealed that the proposed coating film improved the mechanical strength of the NPK fertilizer and enhanced water retention capacity of the soil.					
37224897	1	31	theme	nutrient	187:194	arg1	availability					196:207	Soil nutrient availability	182:207	Soil nutrient availability	182:207	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	0	32	theme	Chitosan/kaolinite	0:17	arg1	clay					19:22	Chitosan/kaolinite clay	0:22	Chitosan/kaolinite clay	0:22	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	1	33	theme	direct	293:298	arg1	impact					300:305	a direct impact	291:305	a direct impact	291:305	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	7	34	theme	coating	1076:1082	arg1	film					1084:1087	the proposed coating film	1063:1087	the proposed coating film	1063:1087	Results revealed that the proposed coating film improved the mechanical strength of the NPK fertilizer and enhanced water retention capacity of the soil.					
37224897	5	35	theme	nutrient	849:856	arg1	release					858:864	the sustained nutrient release	835:864	the sustained nutrient release of coated NPK fertilizer (NPK/CsGC)	835:900	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	4	36	theme	reinforcing	751:761	arg1	composite					671:679	Chitosan composite	662:679	Chitosan composite (CsGC)	662:686	For this purpose, Chitosan composite (CsGC) was adopted as a durable coating material, including clay as a reinforcing coating material.					
37224897	4	36	theme	reinforcing	751:761	arg1	material					771:778	a reinforcing coating material	749:778	a reinforcing coating material	749:778	For this purpose, Chitosan composite (CsGC) was adopted as a durable coating material, including clay as a reinforcing coating material.					
37224897	2	37	theme	obviating	398:406	arg1	effects					416:422	obviating adverse effects	398:422	obviating adverse effects of over-fertilization on soil nutrients and, consequently, on crop yields	398:496	Proper fertilization practices can lead to obviating adverse effects of over-fertilization on soil nutrients and, consequently, on crop yields.					
37224897	5	38	theme	coating	817:823	arg1	influence					785:793	The influence	781:793	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC)	781:900	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	5	39	theme	composite	807:815	arg1	CsGC					826:829	CsGC	826:829	CsGC	826:829	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	5	39	theme	composite	807:815	arg1	coating					817:823	chitosan composite coating	798:823	chitosan composite coating (CsGC)	798:830	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	10	40	theme	coating	1500:1506	arg1	system					1508:1513	the coating system	1496:1513	the coating system	1496:1513	Therefore, kaolinite clay, as part of the coating system, can be an effective way to improve tomato quality and maintain soil nutrients during tomato ripening.					
37224897	8	41	theme	agronomic	1199:1207	arg1	investigation					1209:1221	The agronomic investigation	1195:1221	The agronomic investigation	1195:1221	The agronomic investigation has also proved their outstanding potential to boost chlorophyll content, biomass, and tomato metabolism.					
37224897	10	42	theme	kaolinite	1469:1477	arg1	way					1536:1538	an effective way	1523:1538	an effective way to improve tomato quality and maintain soil nutrients during tomato ripening	1523:1615	Therefore, kaolinite clay, as part of the coating system, can be an effective way to improve tomato quality and maintain soil nutrients during tomato ripening.					
37224897	10	42	theme	kaolinite	1469:1477	arg1	clay					1479:1482	kaolinite clay	1469:1482	kaolinite clay	1469:1482	Therefore, kaolinite clay, as part of the coating system, can be an effective way to improve tomato quality and maintain soil nutrients during tomato ripening.					
37224897	7	43	theme	water	1157:1161	arg1	capacity					1173:1180	water retention capacity	1157:1180	water retention capacity of the soil	1157:1192	Results revealed that the proposed coating film improved the mechanical strength of the NPK fertilizer and enhanced water retention capacity of the soil.					
37224897	6	44	theme	energy-dispersive	948:964	arg1	SEM/EDX					986:992	SEM/EDX	986:992	SEM/EDX	986:992	Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were used to examine the coated NPK granules.					
37224897	6	44	theme	energy-dispersive	948:964	arg1	spectroscopy					972:983	energy-dispersive X-ray spectroscopy	948:983	energy-dispersive X-ray spectroscopy (SEM/EDX)	948:993	Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were used to examine the coated NPK granules.					
37224897	2	45	theme	soil	449:452	arg1	nutrients					454:462	soil nutrients	449:462	soil nutrients	449:462	Proper fertilization practices can lead to obviating adverse effects of over-fertilization on soil nutrients and, consequently, on crop yields.					
37224897	0	46	theme	sustainable	42:52	arg1	material					95:102	a sustainable and environmentally eco-friendly coating material	40:102	a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth	40:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	1	47	theme	crop	310:313	arg1	production					315:324	crop production	310:324	crop production	310:324	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	0	48	theme	soil	148:151	arg1	nutrients					153:161	soil nutrients	148:161	soil nutrients	148:161	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	8	49	theme	chlorophyll	1276:1286	arg1	content					1288:1294	chlorophyll content	1276:1294	chlorophyll content	1276:1294	The agronomic investigation has also proved their outstanding potential to boost chlorophyll content, biomass, and tomato metabolism.					
37224897	3	50	theme	nutrient	603:610	arg1	availability					612:623	soil nutrient availability	598:623	soil nutrient availability	598:623	This work aims to assess the effect of a durable liner material based on biodegradable polymers on soil nutrient availability and tomato growth.					
37224897	10	51	theme	soil	1579:1582	arg1	nutrients					1584:1592	soil nutrients	1579:1592	soil nutrients	1579:1592	Therefore, kaolinite clay, as part of the coating system, can be an effective way to improve tomato quality and maintain soil nutrients during tomato ripening.					
37224897	6	52	theme	NPK	1027:1029	arg1	granules					1031:1038	the coated NPK granules	1016:1038	the coated NPK granules	1016:1038	Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were used to examine the coated NPK granules.					
37224897	9	53	theme	response	1354:1361	arg1	study					1363:1367	the surface response study	1342:1367	the surface response study	1342:1367	Furthermore, the surface response study confirmed a strong correlation between tomato quality and representative soil nutrients.					
37224897	0	54	from	Effect	138:143	arg1	nutrients					153:161	soil nutrients	148:161	soil nutrients	148:161	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	2	55	theme	over-fertilization	427:444	arg1	effects					416:422	obviating adverse effects	398:422	obviating adverse effects of over-fertilization on soil nutrients and, consequently, on crop yields	398:496	Proper fertilization practices can lead to obviating adverse effects of over-fertilization on soil nutrients and, consequently, on crop yields.					
37224897	2	56	theme	crop	486:489	arg1	yields					491:496	crop yields	486:496	crop yields	486:496	Proper fertilization practices can lead to obviating adverse effects of over-fertilization on soil nutrients and, consequently, on crop yields.					
37224897	6	57	theme	electron	924:931	arg1	microscopy					933:942	Scanning electron microscopy	915:942	Scanning electron microscopy	915:942	Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were used to examine the coated NPK granules.					
37224897	6	58	theme	X-ray	966:970	arg1	SEM/EDX					986:992	SEM/EDX	986:992	SEM/EDX	986:992	Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were used to examine the coated NPK granules.					
37224897	6	58	theme	X-ray	966:970	arg1	spectroscopy					972:983	energy-dispersive X-ray spectroscopy	948:983	energy-dispersive X-ray spectroscopy (SEM/EDX)	948:993	Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were used to examine the coated NPK granules.					
37224897	2	59	theme	fertilization	362:374	arg1	practices					376:384	Proper fertilization practices	355:384	Proper fertilization practices	355:384	Proper fertilization practices can lead to obviating adverse effects of over-fertilization on soil nutrients and, consequently, on crop yields.					
37224897	7	60	theme	NPK	1129:1131	arg1	fertilizer					1133:1142	the NPK fertilizer	1125:1142	the NPK fertilizer	1125:1142	Results revealed that the proposed coating film improved the mechanical strength of the NPK fertilizer and enhanced water retention capacity of the soil.					
37224897	1	61	theme	soil	330:333	arg1	quality					346:352	soil ecological quality	330:352	soil ecological quality	330:352	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	5	62	from	influence	785:793	arg1	release					858:864	the sustained nutrient release	835:864	the sustained nutrient release of coated NPK fertilizer (NPK/CsGC)	835:900	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	3	63	theme	soil	598:601	arg1	availability					612:623	soil nutrient availability	598:623	soil nutrient availability	598:623	This work aims to assess the effect of a durable liner material based on biodegradable polymers on soil nutrient availability and tomato growth.					
37224897	2	64	theme	Proper	355:360	arg1	practices					376:384	Proper fertilization practices	355:384	Proper fertilization practices	355:384	Proper fertilization practices can lead to obviating adverse effects of over-fertilization on soil nutrients and, consequently, on crop yields.					
37224897	1	65	theme	ecological	335:344	arg1	quality					346:352	soil ecological quality	330:352	soil ecological quality	330:352	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	8	66	theme	outstanding	1245:1255	arg1	potential					1257:1265	their outstanding potential	1239:1265	their outstanding potential	1239:1265	The agronomic investigation has also proved their outstanding potential to boost chlorophyll content, biomass, and tomato metabolism.					
37224897	9	67	theme	strong	1381:1386	arg1	correlation					1388:1398	a strong correlation	1379:1398	a strong correlation between tomato quality and representative soil nutrients	1379:1455	Furthermore, the surface response study confirmed a strong correlation between tomato quality and representative soil nutrients.					
37224897	1	68	theme	polymer-based	240:252	arg1	fertilizers					267:277	some polymer-based slow-release fertilizers	235:277	some polymer-based slow-release fertilizers (SRFs)	235:284	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	1	68	theme	polymer-based	240:252	arg1	SRFs					280:283	SRFs	280:283	SRFs	280:283	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	0	69	theme	tomato	167:172	arg1	fertilizers					125:135	slow release NPK fertilizers	108:135	slow release NPK fertilizers: Effect on soil nutrients and tomato growth	108:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	0	69	theme	tomato	167:172	arg1	growth					174:179	tomato growth	167:179	tomato growth	167:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	1	70	contain	have	286:289	arg1	non-biodegradation					213:230	non-biodegradation	213:230	non-biodegradation of some polymer-based slow-release fertilizers (SRFs)	213:284	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	1	70	contain	have	286:289	arg1	availability					196:207	Soil nutrient availability	182:207	Soil nutrient availability	182:207	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	1	70	contain	have	286:289	arg2	impact					300:305	a direct impact	291:305	a direct impact	291:305	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	4	71	theme	coating	713:719	arg1	composite					671:679	Chitosan composite	662:679	Chitosan composite (CsGC)	662:686	For this purpose, Chitosan composite (CsGC) was adopted as a durable coating material, including clay as a reinforcing coating material.					
37224897	4	71	theme	coating	713:719	arg1	material					721:728	a durable coating material	703:728	a durable coating material	703:728	For this purpose, Chitosan composite (CsGC) was adopted as a durable coating material, including clay as a reinforcing coating material.					
37224897	1	72	theme	slow-release	254:265	arg1	fertilizers					267:277	some polymer-based slow-release fertilizers	235:277	some polymer-based slow-release fertilizers (SRFs)	235:284	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	1	72	theme	slow-release	254:265	arg1	SRFs					280:283	SRFs	280:283	SRFs	280:283	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	0	73	theme	coating	87:93	arg1	material					95:102	a sustainable and environmentally eco-friendly coating material	40:102	a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth	40:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	6	74	theme	coated	1020:1025	arg1	granules					1031:1038	the coated NPK granules	1016:1038	the coated NPK granules	1016:1038	Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were used to examine the coated NPK granules.					
37224897	5	75	theme	coated	869:874	arg1	fertilizer					880:889	coated NPK fertilizer	869:889	coated NPK fertilizer (NPK/CsGC)	869:900	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	5	75	theme	coated	869:874	arg1	NPK/CsGC					892:899	NPK/CsGC	892:899	NPK/CsGC	892:899	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	3	76	from	polymers	586:593	arg1	availability					612:623	soil nutrient availability	598:623	soil nutrient availability	598:623	This work aims to assess the effect of a durable liner material based on biodegradable polymers on soil nutrient availability and tomato growth.					
37224897	3	76	from	polymers	586:593	arg1	growth					636:641	tomato growth	629:641	tomato growth	629:641	This work aims to assess the effect of a durable liner material based on biodegradable polymers on soil nutrient availability and tomato growth.					
37224897	1	77	theme	fertilizers	267:277	arg1	non-biodegradation					213:230	non-biodegradation	213:230	non-biodegradation of some polymer-based slow-release fertilizers (SRFs)	213:284	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	1	77	theme	fertilizers	267:277	arg1	availability					196:207	Soil nutrient availability	182:207	Soil nutrient availability	182:207	Soil nutrient availability and non-biodegradation of some polymer-based slow-release fertilizers (SRFs) have a direct impact on crop production and soil ecological quality.					
37224897	0	78	theme	eco-friendly	74:85	arg1	material					95:102	a sustainable and environmentally eco-friendly coating material	40:102	a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth	40:179	Chitosan/kaolinite clay biocomposite as a sustainable and environmentally eco-friendly coating material for slow release NPK fertilizers: Effect on soil nutrients and tomato growth.					
37224897	3	79	theme	liner	548:552	arg1	material					554:561	a durable liner material	538:561	a durable liner material	538:561	This work aims to assess the effect of a durable liner material based on biodegradable polymers on soil nutrient availability and tomato growth.					
37224897	3	80	theme	tomato	629:634	arg1	growth					636:641	tomato growth	629:641	tomato growth	629:641	This work aims to assess the effect of a durable liner material based on biodegradable polymers on soil nutrient availability and tomato growth.					
37224897	5	81	theme	NPK	876:878	arg1	fertilizer					880:889	coated NPK fertilizer	869:889	coated NPK fertilizer (NPK/CsGC)	869:900	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	5	81	theme	NPK	876:878	arg1	NPK/CsGC					892:899	NPK/CsGC	892:899	NPK/CsGC	892:899	The influence of chitosan composite coating (CsGC) on the sustained nutrient release of coated NPK fertilizer (NPK/CsGC) was studied.					
37224897	9	82	theme	tomato	1408:1413	arg1	quality					1415:1421	tomato quality	1408:1421	tomato quality	1408:1421	Furthermore, the surface response study confirmed a strong correlation between tomato quality and representative soil nutrients.					
36709817	0	0	theme	simulated	77:85	arg1	endosperm					93:101	simulated wheat endosperm	77:101	simulated wheat endosperm	77:101	Characteristics of the composite film of arabinoxylan and starch granules in simulated wheat endosperm.					
36709817	6	1	theme	grain	940:944	arg1	regulation					952:961	wheat grain water regulation	934:961	wheat grain water regulation	934:961	These findings provide a basis for further studies in the context of wheat grain water regulation.					
36709817	1	2	theme	wheat	142:146	arg1	grains					148:153	wheat grains	142:153	wheat grains	142:153	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	6	3	theme	further	900:906	arg1	studies					908:914	further studies	900:914	further studies in the context of wheat grain water regulation	900:961	These findings provide a basis for further studies in the context of wheat grain water regulation.					
36709817	1	4	theme	grains	148:153	arg1	components					128:137	cell wall components	118:137	cell wall components of wheat grains	118:153	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	0	5	from	Characteristics	0:14	arg1	endosperm					93:101	simulated wheat endosperm	77:101	simulated wheat endosperm	77:101	Characteristics of the composite film of arabinoxylan and starch granules in simulated wheat endosperm.					
36709817	3	6	theme	structural	561:570	arg1	stability					572:580	structural stability	561:580	structural stability	561:580	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	1	7	theme	water	270:274	arg1	content					276:282	water content	270:282	water content	270:282	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	0	8	theme	wheat	87:91	arg1	endosperm					93:101	simulated wheat endosperm	77:101	simulated wheat endosperm	77:101	Characteristics of the composite film of arabinoxylan and starch granules in simulated wheat endosperm.					
36709817	3	9	dep	crystallinity	543:555	arg1	the					539:541	the	539:541	the	539:541	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	4	10	theme	diffusion	642:650	arg1	experiments					652:662	Water diffusion experiments	636:662	Water diffusion experiments of the films	636:675	Water diffusion experiments of the films revealed that the water diffusion rate gradually decreased with increasing starch content.					
36709817	0	11	from	film	33:36	arg1	endosperm					93:101	simulated wheat endosperm	77:101	simulated wheat endosperm	77:101	Characteristics of the composite film of arabinoxylan and starch granules in simulated wheat endosperm.					
36709817	3	12	theme	thermogravimetric	494:510	arg1	TGA					522:524	TGA	522:524	TGA	522:524	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	3	12	theme	thermogravimetric	494:510	arg1	analysis					512:519	thermogravimetric analysis	494:519	thermogravimetric analysis (TGA)	494:525	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	3	13	theme	Scanning	426:433	arg1	microscopy					444:453	Scanning electron microscopy	426:453	Scanning electron microscopy (SEM)	426:459	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	3	13	theme	Scanning	426:433	arg1	SEM					456:458	SEM	456:458	SEM	456:458	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	5	14	theme	starch	805:810	arg1	endosperm					812:820	the starch endosperm	801:820	the starch endosperm	801:820	Therefore, the water mobility of the starch endosperm was lower than that of the aleurone layer.					
36709817	6	15	theme	regulation	952:961	arg1	context					923:929	the context	919:929	the context of wheat grain water regulation	919:961	These findings provide a basis for further studies in the context of wheat grain water regulation.					
36709817	1	16	theme	different	185:193	arg1	stages					209:214	different grain-filling stages	185:214	different grain-filling stages	185:214	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	0	17	theme	composite	23:31	arg1	film					33:36	the composite film	19:36	the composite film of arabinoxylan and starch granules in simulated wheat endosperm	19:101	Characteristics of the composite film of arabinoxylan and starch granules in simulated wheat endosperm.					
36709817	1	18	theme	water	288:292	arg1	p < 0.05					310:317	p < 0.05	310:317	p < 0.05	310:317	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	1	18	theme	water	288:292	arg1	rate					304:307	water migration rate	288:307	water migration rate (p < 0.05)	288:318	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	4	19	theme	starch	752:757	arg1	content					759:765	starch content	752:765	starch content	752:765	Water diffusion experiments of the films revealed that the water diffusion rate gradually decreased with increasing starch content.					
36709817	1	20	theme	grain-filling	195:207	arg1	stages					209:214	different grain-filling stages	185:214	different grain-filling stages	185:214	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	1	21	theme	migration	294:302	arg1	p < 0.05					310:317	p < 0.05	310:317	p < 0.05	310:317	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	1	21	theme	migration	294:302	arg1	rate					304:307	water migration rate	288:307	water migration rate (p < 0.05)	288:318	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	2	22	theme	arabinoxylan	341:352	arg1	film					333:336	A composite film	321:336	A composite film of arabinoxylan and starch granules	321:372	A composite film of arabinoxylan and starch granules was prepared to simulate wheat endosperm structure.					
36709817	3	23	theme	X-ray	462:466	arg1	XRD					484:486	XRD	484:486	XRD	484:486	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	3	23	theme	X-ray	462:466	arg1	diffractometer					468:481	X-ray diffractometer	462:481	X-ray diffractometer (XRD)	462:487	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	6	24	theme	wheat	934:938	arg1	regulation					952:961	wheat grain water regulation	934:961	wheat grain water regulation	934:961	These findings provide a basis for further studies in the context of wheat grain water regulation.					
36709817	1	25	from	differences	255:265	arg1	p < 0.05					310:317	p < 0.05	310:317	p < 0.05	310:317	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	1	25	from	differences	255:265	arg1	rate					304:307	water migration rate	288:307	water migration rate (p < 0.05)	288:318	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	1	25	from	differences	255:265	arg1	content					276:282	water content	270:282	water content	270:282	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	4	26	theme	Water	636:640	arg1	experiments					652:662	Water diffusion experiments	636:662	Water diffusion experiments of the films	636:675	Water diffusion experiments of the films revealed that the water diffusion rate gradually decreased with increasing starch content.					
36709817	3	27	theme	electron	435:442	arg1	microscopy					444:453	Scanning electron microscopy	426:453	Scanning electron microscopy (SEM)	426:459	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	3	27	theme	electron	435:442	arg1	SEM					456:458	SEM	456:458	SEM	456:458	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	2	28	theme	endosperm	405:413	arg1	structure					415:423	wheat endosperm structure	399:423	wheat endosperm structure	399:423	A composite film of arabinoxylan and starch granules was prepared to simulate wheat endosperm structure.					
36709817	0	29	theme	film	33:36	arg1	Characteristics					0:14	Characteristics	0:14	Characteristics of the composite film of arabinoxylan and starch granules in simulated wheat endosperm.	0:102	Characteristics of the composite film of arabinoxylan and starch granules in simulated wheat endosperm.					
36709817	5	30	theme	water	783:787	arg1	mobility					789:796	the water mobility	779:796	the water mobility of the starch endosperm	779:820	Therefore, the water mobility of the starch endosperm was lower than that of the aleurone layer.					
36709817	5	30	theme	water	783:787	arg1	lower					826:830	lower	826:830	lower	826:830	Therefore, the water mobility of the starch endosperm was lower than that of the aleurone layer.					
36709817	3	31	theme	starch	620:625	arg1	content					627:633	starch content	620:633	starch content	620:633	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	5	32	theme	endosperm	812:820	arg1	mobility					789:796	the water mobility	779:796	the water mobility of the starch endosperm	779:820	Therefore, the water mobility of the starch endosperm was lower than that of the aleurone layer.					
36709817	5	32	theme	endosperm	812:820	arg1	lower					826:830	lower	826:830	lower	826:830	Therefore, the water mobility of the starch endosperm was lower than that of the aleurone layer.					
36709817	2	33	theme	wheat	399:403	arg1	structure					415:423	wheat endosperm structure	399:423	wheat endosperm structure	399:423	A composite film of arabinoxylan and starch granules was prepared to simulate wheat endosperm structure.					
36709817	6	34	theme	water	946:950	arg1	regulation					952:961	wheat grain water regulation	934:961	wheat grain water regulation	934:961	These findings provide a basis for further studies in the context of wheat grain water regulation.					
36709817	1	35	dep	differed	155:162	arg1	observed					234:241	observed	234:241	observed significant differences in water content and water migration rate (p < 0.05)	234:318	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	4	36	theme	diffusion	701:709	arg1	rate					711:714	the water diffusion rate	691:714	the water diffusion rate	691:714	Water diffusion experiments of the films revealed that the water diffusion rate gradually decreased with increasing starch content.					
36709817	0	37	theme	arabinoxylan	41:52	arg1	film					33:36	the composite film	19:36	the composite film of arabinoxylan and starch granules in simulated wheat endosperm	19:101	Characteristics of the composite film of arabinoxylan and starch granules in simulated wheat endosperm.					
36709817	4	38	theme	water	695:699	arg1	rate					711:714	the water diffusion rate	691:714	the water diffusion rate	691:714	Water diffusion experiments of the films revealed that the water diffusion rate gradually decreased with increasing starch content.					
36709817	4	39	theme	films	671:675	arg1	experiments					652:662	Water diffusion experiments	636:662	Water diffusion experiments of the films	636:675	Water diffusion experiments of the films revealed that the water diffusion rate gradually decreased with increasing starch content.					
36709817	1	40	theme	cell	118:121	arg1	components					128:137	cell wall components	118:137	cell wall components of wheat grains	118:153	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	0	41	from	endosperm	93:101	arg1	Characteristics					0:14	Characteristics	0:14	Characteristics of the composite film of arabinoxylan and starch granules in simulated wheat endosperm.	0:102	Characteristics of the composite film of arabinoxylan and starch granules in simulated wheat endosperm.					
36709817	1	42	theme	wall	123:126	arg1	components					128:137	cell wall components	118:137	cell wall components of wheat grains	118:153	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36709817	2	43	theme	composite	323:331	arg1	film					333:336	A composite film	321:336	A composite film of arabinoxylan and starch granules	321:372	A composite film of arabinoxylan and starch granules was prepared to simulate wheat endosperm structure.					
36709817	0	44	theme	granules	65:72	arg1	film					33:36	the composite film	19:36	the composite film of arabinoxylan and starch granules in simulated wheat endosperm	19:101	Characteristics of the composite film of arabinoxylan and starch granules in simulated wheat endosperm.					
36709817	3	45	theme	film	589:592	arg1	crystallinity					543:555	crystallinity	543:555	crystallinity	543:555	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	3	45	theme	film	589:592	arg1	stability					572:580	structural stability	561:580	structural stability	561:580	Scanning electron microscopy (SEM), X-ray diffractometer (XRD), and thermogravimetric analysis (TGA) showed that the crystallinity and structural stability of the film increased with increasing starch content.					
36709817	2	46	theme	granules	365:372	arg1	film					333:336	A composite film	321:336	A composite film of arabinoxylan and starch granules	321:372	A composite film of arabinoxylan and starch granules was prepared to simulate wheat endosperm structure.					
36709817	5	47	theme	aleurone	849:856	arg1	layer					858:862	the aleurone layer	845:862	the aleurone layer	845:862	Therefore, the water mobility of the starch endosperm was lower than that of the aleurone layer.					
36709817	0	48	theme	starch	58:63	arg1	granules					65:72	starch granules	58:72	starch granules	58:72	Characteristics of the composite film of arabinoxylan and starch granules in simulated wheat endosperm.					
36709817	2	49	theme	starch	358:363	arg1	granules					365:372	starch granules	358:372	starch granules	358:372	A composite film of arabinoxylan and starch granules was prepared to simulate wheat endosperm structure.					
36709817	6	50	from	studies	908:914	arg1	context					923:929	the context	919:929	the context of wheat grain water regulation	919:961	These findings provide a basis for further studies in the context of wheat grain water regulation.					
36709817	1	51	theme	significant	243:253	arg1	differences					255:265	significant differences	243:265	significant differences in water content and water migration rate (p < 0.05)	243:318	We found that cell wall components of wheat grains differed significantly across different grain-filling stages; specifically, we observed significant differences in water content and water migration rate (p < 0.05).					
36044353	0	0	theme	Cisplatin	101:109	arg1	Delivery					111:118	Cisplatin Delivery	101:118	Cisplatin Delivery	101:118	Polyphenol-Incorporated Composite Nanogels of Multimodal Interactions for Enhanced Gel Stability and Cisplatin Delivery.					
36044353	2	1	theme	polymer	528:534	arg1	networks					536:543	physically cross-linked polymer networks	504:543	physically cross-linked polymer networks	504:543	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	1	2	theme	facile	181:186	arg1	cross-linking					188:200	the facile cross-linking	177:200	the facile cross-linking of polymer chains	177:218	Naturally abundant polyphenols that can readily undergo the facile cross-linking of polymer chains have been of great interest for functional hydrogel formation and its potential biomedical applications.					
36044353	2	3	theme	cross-linked	515:526	arg1	networks					536:543	physically cross-linked polymer networks	504:543	physically cross-linked polymer networks	504:543	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	2	4	theme	acid	385:388	arg1	incorporation					361:373	the internal incorporation	348:373	the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates	348:466	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	3	5	theme	Stern-Volmer	899:910	arg1	analysis					912:919	the Stern-Volmer analysis	895:919	the Stern-Volmer analysis of the TA-induced quenching property	895:956	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	2	6	from	stability	589:597	arg1	solution					610:617	protein solution	602:617	protein solution	602:617	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	4	7	theme	components	1201:1210	arg1	delivery					1180:1187	the potential delivery	1166:1187	the potential delivery of metallic components	1166:1210	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	4	7	theme	components	1201:1210	arg1	release					1154:1160	acid-sensitive drug release	1134:1160	acid-sensitive drug release	1134:1160	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	1	8	theme	potential	290:298	arg1	applications					311:322	its potential biomedical applications	286:322	its potential biomedical applications	286:322	Naturally abundant polyphenols that can readily undergo the facile cross-linking of polymer chains have been of great interest for functional hydrogel formation and its potential biomedical applications.					
36044353	4	9	theme	coordination	986:997	arg1	generality					999:1008	the excellent coordination generality	972:1008	the excellent coordination generality of TA for versatile metallic cations	972:1045	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	3	10	theme	diluted	747:753	arg1	solution					763:770	diluted nanogel solution	747:770	diluted nanogel solution	747:770	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	3	11	from	emission	819:826	arg1	networks					863:870	polymer networks	855:870	polymer networks	855:870	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	3	12	theme	property	949:956	arg1	analysis					912:919	the Stern-Volmer analysis	895:919	the Stern-Volmer analysis of the TA-induced quenching property	895:956	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	4	13	theme	TA	1013:1014	arg1	generality					999:1008	the excellent coordination generality	972:1008	the excellent coordination generality of TA for versatile metallic cations	972:1045	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	3	14	theme	nanogel	755:761	arg1	solution					763:770	diluted nanogel solution	747:770	diluted nanogel solution	747:770	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	4	15	theme	metallic	1192:1199	arg1	components					1201:1210	metallic components	1192:1210	metallic components	1192:1210	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	3	16	theme	polymer	855:861	arg1	networks					863:870	polymer networks	855:870	polymer networks	855:870	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	3	17	theme	TA-induced	928:937	arg1	property					949:956	the TA-induced quenching property	924:956	the TA-induced quenching property	924:956	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	1	18	theme	polymer	205:211	arg1	chains					213:218	polymer chains	205:218	polymer chains	205:218	Naturally abundant polyphenols that can readily undergo the facile cross-linking of polymer chains have been of great interest for functional hydrogel formation and its potential biomedical applications.					
36044353	0	19	theme	Composite	24:32	arg1	Nanogels					34:41	Polyphenol-Incorporated Composite Nanogels	0:41	Polyphenol-Incorporated Composite Nanogels of Multimodal Interactions for Enhanced Gel Stability and Cisplatin Delivery.	0:119	Polyphenol-Incorporated Composite Nanogels of Multimodal Interactions for Enhanced Gel Stability and Cisplatin Delivery.					
36044353	4	20	theme	metallic	1030:1037	arg1	cations					1039:1045	versatile metallic cations	1020:1045	versatile metallic cations	1020:1045	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	3	21	theme	quenching	939:947	arg1	property					949:956	the TA-induced quenching property	924:956	the TA-induced quenching property	924:956	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	1	22	theme	chains	213:218	arg1	cross-linking					188:200	the facile cross-linking	177:200	the facile cross-linking of polymer chains	177:218	Naturally abundant polyphenols that can readily undergo the facile cross-linking of polymer chains have been of great interest for functional hydrogel formation and its potential biomedical applications.					
36044353	0	23	theme	Polyphenol-Incorporated	0:22	arg1	Nanogels					34:41	Polyphenol-Incorporated Composite Nanogels	0:41	Polyphenol-Incorporated Composite Nanogels of Multimodal Interactions for Enhanced Gel Stability and Cisplatin Delivery.	0:119	Polyphenol-Incorporated Composite Nanogels of Multimodal Interactions for Enhanced Gel Stability and Cisplatin Delivery.					
36044353	4	24	theme	potential	1170:1178	arg1	delivery					1180:1187	the potential delivery	1166:1187	the potential delivery of metallic components	1166:1210	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	4	25	theme	pharmacophore	1096:1108	arg1	immobilization					1066:1079	the facile immobilization	1055:1079	the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components	1055:1210	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	2	26	theme	gel	585:587	arg1	stability					589:597	the colloidal gel stability	571:597	the colloidal gel stability in protein solution	571:617	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	2	27	theme	chitosan-based	435:448	arg1	templates					458:466	the chitosan-based nanogel templates	431:466	the chitosan-based nanogel templates	431:466	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	2	28	theme	nanogel	450:456	arg1	templates					458:466	the chitosan-based nanogel templates	431:466	the chitosan-based nanogel templates	431:466	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	3	29	theme	solution	763:770	arg1	estimation					733:742	the viscosity estimation	719:742	the viscosity estimation	719:742	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	2	30	theme	colloidal	575:583	arg1	stability					589:597	the colloidal gel stability	571:597	the colloidal gel stability in protein solution	571:617	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	3	31	theme	π-stacking	675:684	arg1	capability					686:695	π-stacking capability	675:695	π-stacking capability	675:695	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	1	32	theme	abundant	131:138	arg1	polyphenols					140:150	Naturally abundant polyphenols	121:150	Naturally abundant polyphenols that can readily undergo the facile cross-linking of polymer chains	121:218	Naturally abundant polyphenols that can readily undergo the facile cross-linking of polymer chains have been of great interest for functional hydrogel formation and its potential biomedical applications.					
36044353	3	33	from	networks	863:870	arg1	emission					819:826	the conformation-dependent excimer emission	784:826	the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property	784:956	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	0	34	theme	Interactions	57:68	arg1	Nanogels					34:41	Polyphenol-Incorporated Composite Nanogels	0:41	Polyphenol-Incorporated Composite Nanogels of Multimodal Interactions for Enhanced Gel Stability and Cisplatin Delivery.	0:119	Polyphenol-Incorporated Composite Nanogels of Multimodal Interactions for Enhanced Gel Stability and Cisplatin Delivery.					
36044353	4	35	theme	excellent	976:984	arg1	generality					999:1008	the excellent coordination generality	972:1008	the excellent coordination generality of TA for versatile metallic cations	972:1045	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	1	36	theme	great	233:237	arg1	interest					239:246	great interest	233:246	great interest	233:246	Naturally abundant polyphenols that can readily undergo the facile cross-linking of polymer chains have been of great interest for functional hydrogel formation and its potential biomedical applications.					
36044353	0	37	theme	Multimodal	46:55	arg1	Interactions					57:68	Multimodal Interactions	46:68	Multimodal Interactions	46:68	Polyphenol-Incorporated Composite Nanogels of Multimodal Interactions for Enhanced Gel Stability and Cisplatin Delivery.					
36044353	3	38	theme	excimer	811:817	arg1	emission					819:826	the conformation-dependent excimer emission	784:826	the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property	784:956	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	2	39	theme	additive	419:426	arg1	polyphenol					408:417	a natural polyphenol	398:417	a natural polyphenol additive at the chitosan-based nanogel templates	398:466	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	0	40	theme	Enhanced	74:81	arg1	Stability					87:95	Enhanced Gel Stability	74:95	Enhanced Gel Stability	74:95	Polyphenol-Incorporated Composite Nanogels of Multimodal Interactions for Enhanced Gel Stability and Cisplatin Delivery.					
36044353	0	41	theme	Gel	83:85	arg1	Stability					87:95	Enhanced Gel Stability	74:95	Enhanced Gel Stability	74:95	Polyphenol-Incorporated Composite Nanogels of Multimodal Interactions for Enhanced Gel Stability and Cisplatin Delivery.					
36044353	2	42	link	cross-linked	515:526	arg1	networks					536:543	physically cross-linked polymer networks	504:543	physically cross-linked polymer networks	504:543	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	2	43	theme	tannic	378:383	arg1	TA					391:392	TA	391:392	TA	391:392	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	2	43	theme	tannic	378:383	arg1	acid					385:388	tannic acid	378:388	tannic acid (TA)	378:393	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	4	44	theme	facile	1059:1064	arg1	immobilization					1066:1079	the facile immobilization	1055:1079	the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components	1055:1210	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	2	45	theme	protein	602:608	arg1	solution					610:617	protein solution	602:617	protein solution	602:617	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	1	46	dep	potential	290:298	arg1	biomedical					300:309	biomedical	300:309	biomedical	300:309	Naturally abundant polyphenols that can readily undergo the facile cross-linking of polymer chains have been of great interest for functional hydrogel formation and its potential biomedical applications.					
36044353	4	47	theme	drug	1149:1152	arg1	release					1154:1160	acid-sensitive drug release	1134:1160	acid-sensitive drug release	1134:1160	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	3	48	theme	conformation-dependent	788:809	arg1	emission					819:826	the conformation-dependent excimer emission	784:826	the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property	784:956	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	3	49	from	fluorophores	839:850	arg1	networks					863:870	polymer networks	855:870	polymer networks	855:870	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	2	50	from	dilution	550:557	arg1	solution					610:617	protein solution	602:617	protein solution	602:617	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	3	51	theme	multivalent	642:652	arg1	bonding					663:669	the multivalent hydrogen bonding	638:669	the multivalent hydrogen bonding	638:669	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	4	52	theme	acid-sensitive	1134:1147	arg1	release					1154:1160	acid-sensitive drug release	1134:1160	acid-sensitive drug release	1134:1160	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	3	53	theme	fluorophores	839:850	arg1	emission					819:826	the conformation-dependent excimer emission	784:826	the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property	784:956	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	3	54	theme	hydrogen	654:661	arg1	bonding					663:669	the multivalent hydrogen bonding	638:669	the multivalent hydrogen bonding	638:669	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	2	55	theme	internal	352:359	arg1	incorporation					361:373	the internal incorporation	348:373	the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates	348:466	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	1	56	theme	functional	252:261	arg1	formation					272:280	functional hydrogel formation	252:280	functional hydrogel formation	252:280	Naturally abundant polyphenols that can readily undergo the facile cross-linking of polymer chains have been of great interest for functional hydrogel formation and its potential biomedical applications.					
36044353	4	57	theme	versatile	1020:1028	arg1	cations					1039:1045	versatile metallic cations	1020:1045	versatile metallic cations	1020:1045	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	3	58	theme	TA	700:701	arg1	bonding					663:669	the multivalent hydrogen bonding	638:669	the multivalent hydrogen bonding	638:669	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	3	58	theme	TA	700:701	arg1	capability					686:695	π-stacking capability	675:695	π-stacking capability	675:695	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	2	59	theme	natural	400:406	arg1	polyphenol					408:417	a natural polyphenol	398:417	a natural polyphenol additive at the chitosan-based nanogel templates	398:466	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	1	60	theme	hydrogel	263:270	arg1	formation					272:280	functional hydrogel formation	252:280	functional hydrogel formation	252:280	Naturally abundant polyphenols that can readily undergo the facile cross-linking of polymer chains have been of great interest for functional hydrogel formation and its potential biomedical applications.					
36044353	2	61	theme	networks	536:543	arg1	self-association					484:499	the self-association	480:499	the self-association of physically cross-linked polymer networks	480:543	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	2	62	from	templates	458:466	arg1	additive					419:426	additive	419:426	additive	419:426	Herein, we report that the internal incorporation of tannic acid (TA) as a natural polyphenol additive at the chitosan-based nanogel templates can enhance the self-association of physically cross-linked polymer networks upon dilution, as well as the colloidal gel stability in protein solution.					
36044353	4	63	theme	cisplatin	1086:1094	arg1	pharmacophore					1096:1108	a cisplatin pharmacophore	1084:1108	a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components	1084:1210	Furthermore, the excellent coordination generality of TA for versatile metallic cations enables the facile immobilization of a cisplatin pharmacophore inside the nanogels for acid-sensitive drug release and the potential delivery of metallic components.					
36044353	3	64	theme	viscosity	723:731	arg1	estimation					733:742	the viscosity estimation	719:742	the viscosity estimation	719:742	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
36044353	3	65	theme	stacked	831:837	arg1	fluorophores					839:850	stacked fluorophores	831:850	stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property	831:956	This is driven by the multivalent hydrogen bonding and π-stacking capability of TA, as verified by the viscosity estimation of diluted nanogel solution, coupled to the conformation-dependent excimer emission of stacked fluorophores in polymer networks, which also allows for the Stern-Volmer analysis of the TA-induced quenching property.					
35764108	9	0	theme	Pb	1845:1846	arg1	removal					1848:1854	Pb removal	1845:1854	Pb removal from wastewater and soil	1845:1879	Hence, SFM@CBC-350 has the potential of being used as a green, efficient and promising adsorbent in Pb removal from wastewater and soil.					
35764108	8	1	theme	@	1622:1622	arg1	CBC-350					1623:1629	SFM@CBC-350	1619:1629	SFM@CBC-350	1619:1629	Besides, the addition of SFM@CBC-350 could increase the richness and diversity of soil microorganisms, which was beneficial to the growth of ryegrass.					
35764108	2	2	theme	190.17 mg g-1	495:507	arg1	capacity					465:472	the maximum adsorption capacity	442:472	the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1	442:507	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	5	3	theme	@	1090:1090	arg1	CBC					1091:1093	SFM@CBC	1087:1093	SFM@CBC	1087:1093	The reusability test demonstrated that SFM@CBC and SFM@CBC-350 had very good stability and reusability.					
35764108	7	4	theme	Pb	1571:1572	arg1	stress					1574:1579	Pb stress	1571:1579	Pb stress of ryegrass	1571:1591	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	1	5	attach	derived	169:175	arg1	straw					187:191	corn straw	182:191	corn straw (SFM@CBC and SFM@CBC-350)	182:217	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	1	5	attach	derived	169:175	arg2	biochar					161:167	modified biochar	152:167	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350)	116:217	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	1	6	theme	corn	182:185	arg1	straw					187:191	corn straw	182:191	corn straw (SFM@CBC and SFM@CBC-350)	182:217	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	4	7	theme	electrostatic	1001:1013	arg1	attraction					1015:1024	electrostatic attraction	1001:1024	electrostatic attraction	1001:1024	The mechanism studies indicated that Pb2+ removal involved with multiple mechanism, including complexation (dominant process confirmed by XPS analysis), physical adsorption, electrostatic attraction, and cation exchange.					
35764108	3	8	theme	adsorption	620:629	arg1	kinetics					631:638	adsorption kinetics	620:638	adsorption kinetics	620:638	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	2	9	theme	maximum	446:452	arg1	capacity					465:472	the maximum adsorption capacity	442:472	the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1	442:507	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	7	10	theme	ryegrass	1544:1551	arg1	intensity					1531:1539	ROS fluorescence intensity	1514:1539	ROS fluorescence intensity of ryegrass	1514:1551	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	7	10	theme	ryegrass	1544:1551	arg1	activity					1454:1461	the activity	1450:1461	the activity of antioxidant enzymes (CAT, SOD, MDA and POD)	1450:1508	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	1	11	theme	@	197:197	arg1	CBC					198:200	SFM@CBC	194:200	SFM@CBC	194:200	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	8	12	theme	microorganisms	1681:1694	arg1	richness					1650:1657	richness	1650:1657	richness	1650:1657	Besides, the addition of SFM@CBC-350 could increase the richness and diversity of soil microorganisms, which was beneficial to the growth of ryegrass.					
35764108	8	12	theme	microorganisms	1681:1694	arg1	diversity					1663:1671	diversity	1663:1671	diversity	1663:1671	Besides, the addition of SFM@CBC-350 could increase the richness and diversity of soil microorganisms, which was beneficial to the growth of ryegrass.					
35764108	1	13	theme	Pb	307:308	arg1	removal					310:316	Pb removal	307:316	Pb removal from water and soil	307:336	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	2	14	theme	170.91 mg g-1	477:489	arg1	capacity					465:472	the maximum adsorption capacity	442:472	the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1	442:507	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	1	15	theme	Starch-stablized	116:131	arg1	bimetals					143:150	Starch-stablized and Fe/Mn bimetals	116:150	bimetals	143:150	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	7	16	theme	fluorescence	1518:1529	arg1	intensity					1531:1539	ROS fluorescence intensity	1514:1539	ROS fluorescence intensity of ryegrass	1514:1551	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	7	17	theme	total	1415:1419	arg1	content					1433:1439	the biomass and total chlorophyll content	1399:1439	content	1433:1439	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	1	18	theme	Fe/Mn	137:141	arg1	bimetals					143:150	Starch-stablized and Fe/Mn bimetals	116:150	bimetals	143:150	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	3	19	theme	@	714:714	arg1	CBC					715:717	SFM@CBC	711:717	SFM@CBC	711:717	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	4	20	theme	mechanism	831:839	arg1	studies					841:847	The mechanism studies	827:847	The mechanism studies	827:847	The mechanism studies indicated that Pb2+ removal involved with multiple mechanism, including complexation (dominant process confirmed by XPS analysis), physical adsorption, electrostatic attraction, and cation exchange.					
35764108	6	21	theme	biochar	1232:1238	arg1	performance					1204:1214	Pb removal performance	1193:1214	Pb removal performance of the modified biochar	1193:1238	In addition, in order to further explore Pb removal performance of the modified biochar, SFM@CBC-350 was used in soil-ryegrass pot systems.					
35764108	1	22	theme	modified	152:159	arg1	biochar					161:167	modified biochar	152:167	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350)	116:217	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	8	23	dep	richness	1650:1657	arg1	the					1646:1648	the	1646:1648	the	1646:1648	Besides, the addition of SFM@CBC-350 could increase the richness and diversity of soil microorganisms, which was beneficial to the growth of ryegrass.					
35764108	4	24	theme	cation	1031:1036	arg1	exchange					1038:1045	cation exchange	1031:1045	cation exchange	1031:1045	The mechanism studies indicated that Pb2+ removal involved with multiple mechanism, including complexation (dominant process confirmed by XPS analysis), physical adsorption, electrostatic attraction, and cation exchange.					
35764108	1	25	from	water	323:327	arg1	removal					310:316	Pb removal	307:316	Pb removal from water and soil	307:336	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	3	26	theme	@	726:726	arg1	CBC-350					727:733	SFM@CBC-350	723:733	SFM@CBC-350	723:733	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	5	27	theme	good	1120:1123	arg1	stability					1125:1133	stability	1125:1133	stability	1125:1133	The reusability test demonstrated that SFM@CBC and SFM@CBC-350 had very good stability and reusability.					
35764108	4	28	dep	complexation	921:932	arg1	process					944:950	dominant process	935:950	dominant process confirmed by XPS analysis	935:976	The mechanism studies indicated that Pb2+ removal involved with multiple mechanism, including complexation (dominant process confirmed by XPS analysis), physical adsorption, electrostatic attraction, and cation exchange.					
35764108	6	29	theme	Pb	1193:1194	arg1	performance					1204:1214	Pb removal performance	1193:1214	Pb removal performance of the modified biochar	1193:1238	In addition, in order to further explore Pb removal performance of the modified biochar, SFM@CBC-350 was used in soil-ryegrass pot systems.					
35764108	4	30	theme	multiple	891:898	arg1	mechanism					900:908	multiple mechanism	891:908	multiple mechanism	891:908	The mechanism studies indicated that Pb2+ removal involved with multiple mechanism, including complexation (dominant process confirmed by XPS analysis), physical adsorption, electrostatic attraction, and cation exchange.					
35764108	2	31	theme	adsorption	390:399	arg1	performance					401:411	highly effective adsorption performance	373:411	highly effective adsorption performance of Pb2+ from wastewater	373:435	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	6	32	theme	soil-ryegrass	1265:1277	arg1	systems					1283:1289	soil-ryegrass pot systems	1265:1289	soil-ryegrass pot systems	1265:1289	In addition, in order to further explore Pb removal performance of the modified biochar, SFM@CBC-350 was used in soil-ryegrass pot systems.					
35764108	8	33	theme	SFM	1619:1621	arg1	CBC-350					1623:1629	SFM@CBC-350	1619:1629	SFM@CBC-350	1619:1629	Besides, the addition of SFM@CBC-350 could increase the richness and diversity of soil microorganisms, which was beneficial to the growth of ryegrass.					
35764108	7	34	theme	enzymes	1478:1484	arg1	intensity					1531:1539	ROS fluorescence intensity	1514:1539	ROS fluorescence intensity of ryegrass	1514:1551	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	7	34	theme	enzymes	1478:1484	arg1	activity					1454:1461	the activity	1450:1461	the activity of antioxidant enzymes (CAT, SOD, MDA and POD)	1450:1508	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	2	35	theme	SFM	339:341	arg1	CBC					343:345	SFM@CBC	339:345	SFM@CBC	339:345	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	2	36	theme	FM	561:562	arg1	149.25 mg g-1					569:581	149.25 mg g-1	569:581	149.25 mg g-1	569:581	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	2	36	theme	FM	561:562	arg1	CBC					564:566	FM@CBC	561:566	FM@CBC (149.25 mg g-1)	561:582	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	1	37	dep	bimetals	143:150	arg1	biochar					161:167	modified biochar	152:167	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350)	116:217	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	5	38	theme	reusability	1052:1062	arg1	test					1064:1067	The reusability test	1048:1067	The reusability test	1048:1067	The reusability test demonstrated that SFM@CBC and SFM@CBC-350 had very good stability and reusability.					
35764108	7	39	theme	Pb	1356:1357	arg1	content					1359:1365	Pb content	1356:1365	Pb content	1356:1365	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	4	40	theme	Pb2+	864:867	arg1	removal					869:875	Pb2+ removal	864:875	Pb2+ removal	864:875	The mechanism studies indicated that Pb2+ removal involved with multiple mechanism, including complexation (dominant process confirmed by XPS analysis), physical adsorption, electrostatic attraction, and cation exchange.					
35764108	1	41	dep	straw	187:191	arg1	CBC-350					210:216	SFM@CBC-350	206:216	SFM@CBC-350	206:216	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	1	41	dep	straw	187:191	arg1	CBC					198:200	SFM@CBC	194:200	SFM@CBC	194:200	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	0	42	theme	starch-stablized	29:44	arg1	composites					60:69	starch-stablized Fe-Mn/biochar composites	29:69	starch-stablized Fe-Mn/biochar composites	29:69	Synthesis and application of starch-stablized Fe-Mn/biochar composites for the removal of lead from water and soil.					
35764108	7	43	theme	CBC-350	1340:1346	arg1	addition					1324:1331	the addition	1320:1331	the addition of SFM@CBC-350	1320:1346	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	2	44	theme	SFM	351:353	arg1	CBC-350					355:361	SFM@CBC-350	351:361	SFM@CBC-350	351:361	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	1	45	from	soil	333:336	arg1	removal					310:316	Pb removal	307:316	Pb removal from water and soil	307:336	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	0	46	theme	composites	60:69	arg1	application					14:24	application	14:24	application	14:24	Synthesis and application of starch-stablized Fe-Mn/biochar composites for the removal of lead from water and soil.					
35764108	0	46	theme	composites	60:69	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and application of starch-stablized Fe-Mn/biochar composites for the removal of lead from water and soil.					
35764108	2	47	dep	displayed	363:371	arg1	greater					540:546	greater	540:546	greater	540:546	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	7	48	theme	SFM	1336:1338	arg1	CBC-350					1340:1346	SFM@CBC-350	1336:1346	SFM@CBC-350	1336:1346	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	1	49	theme	SFM	206:208	arg1	CBC-350					210:216	SFM@CBC-350	206:216	SFM@CBC-350	206:216	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	4	50	theme	XPS	965:967	arg1	analysis					969:976	XPS analysis	965:976	XPS analysis	965:976	The mechanism studies indicated that Pb2+ removal involved with multiple mechanism, including complexation (dominant process confirmed by XPS analysis), physical adsorption, electrostatic attraction, and cation exchange.					
35764108	2	51	from	wastewater	426:435	arg1	performance					401:411	highly effective adsorption performance	373:411	highly effective adsorption performance of Pb2+ from wastewater	373:435	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	5	52	theme	SFM	1099:1101	arg1	CBC-350					1103:1109	SFM@CBC-350	1099:1109	SFM@CBC-350	1099:1109	The reusability test demonstrated that SFM@CBC and SFM@CBC-350 had very good stability and reusability.					
35764108	7	53	theme	ryegrass	1584:1591	arg1	stress					1574:1579	Pb stress	1571:1579	Pb stress of ryegrass	1571:1591	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	6	54	theme	modified	1223:1230	arg1	biochar					1232:1238	the modified biochar	1219:1238	the modified biochar	1219:1238	In addition, in order to further explore Pb removal performance of the modified biochar, SFM@CBC-350 was used in soil-ryegrass pot systems.					
35764108	8	55	theme	CBC-350	1623:1629	arg1	addition					1607:1614	the addition	1603:1614	the addition of SFM@CBC-350	1603:1629	Besides, the addition of SFM@CBC-350 could increase the richness and diversity of soil microorganisms, which was beneficial to the growth of ryegrass.					
35764108	4	56	theme	dominant	935:942	arg1	process					944:950	dominant process	935:950	dominant process confirmed by XPS analysis	935:976	The mechanism studies indicated that Pb2+ removal involved with multiple mechanism, including complexation (dominant process confirmed by XPS analysis), physical adsorption, electrostatic attraction, and cation exchange.					
35764108	9	57	theme	SFM	1752:1754	arg1	CBC-350					1756:1762	SFM@CBC-350	1752:1762	SFM@CBC-350	1752:1762	Hence, SFM@CBC-350 has the potential of being used as a green, efficient and promising adsorbent in Pb removal from wastewater and soil.					
35764108	5	58	theme	SFM	1087:1089	arg1	CBC					1091:1093	SFM@CBC	1087:1093	SFM@CBC	1087:1093	The reusability test demonstrated that SFM@CBC and SFM@CBC-350 had very good stability and reusability.					
35764108	6	59	theme	SFM	1241:1243	arg1	CBC-350					1245:1251	SFM@CBC-350	1241:1251	SFM@CBC-350	1241:1251	In addition, in order to further explore Pb removal performance of the modified biochar, SFM@CBC-350 was used in soil-ryegrass pot systems.					
35764108	6	60	used	used	1257:1260	arg2	CBC-350					1245:1251	SFM@CBC-350	1241:1251	SFM@CBC-350	1241:1251	In addition, in order to further explore Pb removal performance of the modified biochar, SFM@CBC-350 was used in soil-ryegrass pot systems.					
35764108	3	61	theme	kinetics	631:638	arg1	Studies					609:615	Studies	609:615	Studies of adsorption kinetics, isotherms and thermodynamics	609:668	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	8	62	theme	soil	1676:1679	arg1	microorganisms					1681:1694	soil microorganisms	1676:1694	soil microorganisms	1676:1694	Besides, the addition of SFM@CBC-350 could increase the richness and diversity of soil microorganisms, which was beneficial to the growth of ryegrass.					
35764108	1	63	theme	SFM	194:196	arg1	CBC					198:200	SFM@CBC	194:200	SFM@CBC	194:200	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	4	64	theme	physical	980:987	arg1	adsorption					989:998	physical adsorption	980:998	physical adsorption	980:998	The mechanism studies indicated that Pb2+ removal involved with multiple mechanism, including complexation (dominant process confirmed by XPS analysis), physical adsorption, electrostatic attraction, and cation exchange.					
35764108	7	65	theme	chlorophyll	1421:1431	arg1	content					1433:1439	the biomass and total chlorophyll content	1399:1439	content	1433:1439	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	3	66	theme	thermodynamics	655:668	arg1	Studies					609:615	Studies	609:615	Studies of adsorption kinetics, isotherms and thermodynamics	609:668	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	0	67	from	soil	110:113	arg1	removal					79:85	the removal	75:85	the removal of lead from water and soil	75:113	Synthesis and application of starch-stablized Fe-Mn/biochar composites for the removal of lead from water and soil.					
35764108	3	68	theme	isotherms	641:649	arg1	Studies					609:615	Studies	609:615	Studies of adsorption kinetics, isotherms and thermodynamics	609:668	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	3	69	theme	SFM	723:725	arg1	CBC-350					727:733	SFM@CBC-350	723:733	SFM@CBC-350	723:733	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	9	70	dep	green	1801:1805	arg1	adsorbent					1832:1840	adsorbent	1832:1840	adsorbent	1832:1840	Hence, SFM@CBC-350 has the potential of being used as a green, efficient and promising adsorbent in Pb removal from wastewater and soil.					
35764108	2	71	theme	adsorption	454:463	arg1	capacity					465:472	the maximum adsorption capacity	442:472	the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1	442:507	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	0	72	from	water	100:104	arg1	removal					79:85	the removal	75:85	the removal of lead from water and soil	75:113	Synthesis and application of starch-stablized Fe-Mn/biochar composites for the removal of lead from water and soil.					
35764108	9	73	contain	has	1764:1766	arg1	CBC-350					1756:1762	SFM@CBC-350	1752:1762	SFM@CBC-350	1752:1762	Hence, SFM@CBC-350 has the potential of being used as a green, efficient and promising adsorbent in Pb removal from wastewater and soil.					
35764108	9	73	contain	has	1764:1766	arg2	potential					1772:1780	the potential	1768:1780	the potential of being used as a green, efficient and promising adsorbent in Pb removal from wastewater and soil	1768:1879	Hence, SFM@CBC-350 has the potential of being used as a green, efficient and promising adsorbent in Pb removal from wastewater and soil.					
35764108	5	74	contain	had	1111:1113	arg1	CBC-350					1103:1109	SFM@CBC-350	1099:1109	SFM@CBC-350	1099:1109	The reusability test demonstrated that SFM@CBC and SFM@CBC-350 had very good stability and reusability.					
35764108	5	74	contain	had	1111:1113	arg2	reusability					1139:1149	reusability	1139:1149	reusability	1139:1149	The reusability test demonstrated that SFM@CBC and SFM@CBC-350 had very good stability and reusability.					
35764108	5	74	contain	had	1111:1113	arg2	stability					1125:1133	stability	1125:1133	stability	1125:1133	The reusability test demonstrated that SFM@CBC and SFM@CBC-350 had very good stability and reusability.					
35764108	5	74	contain	had	1111:1113	arg1	CBC					1091:1093	SFM@CBC	1087:1093	SFM@CBC	1087:1093	The reusability test demonstrated that SFM@CBC and SFM@CBC-350 had very good stability and reusability.					
35764108	7	75	theme	ROS	1514:1516	arg1	intensity					1531:1539	ROS fluorescence intensity	1514:1539	ROS fluorescence intensity of ryegrass	1514:1551	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	2	76	theme	effective	380:388	arg1	performance					401:411	highly effective adsorption performance	373:411	highly effective adsorption performance of Pb2+ from wastewater	373:435	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	7	77	dep	enzymes	1478:1484	arg1	POD					1505:1507	POD	1505:1507	POD	1505:1507	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	7	77	dep	enzymes	1478:1484	arg1	enzymes					1478:1484	antioxidant enzymes	1466:1484	antioxidant enzymes (CAT, SOD, MDA and POD)	1466:1508	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	7	77	dep	enzymes	1478:1484	arg1	MDA					1497:1499	MDA	1497:1499	MDA	1497:1499	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	7	77	dep	enzymes	1478:1484	arg1	SOD					1492:1494	SOD	1492:1494	SOD	1492:1494	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	7	77	dep	enzymes	1478:1484	arg1	CAT					1487:1489	CAT	1487:1489	CAT	1487:1489	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	0	78	theme	lead	90:93	arg1	removal					79:85	the removal	75:85	the removal of lead from water and soil	75:113	Synthesis and application of starch-stablized Fe-Mn/biochar composites for the removal of lead from water and soil.					
35764108	3	79	theme	spontaneous	739:749	arg1	reaction					767:774	spontaneous and endothermic reaction	739:774	spontaneous and endothermic reaction	739:774	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	3	79	theme	spontaneous	739:749	arg1	absorption					689:698	the absorption	685:698	the absorption of Pb2+ by SFM@CBC and SFM@CBC-350	685:733	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	1	80	dep	characterized	242:254	arg1	SEM					268:270	SEM	268:270	SEM	268:270	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	1	80	dep	characterized	242:254	arg1	XPS					286:288	XPS	286:288	XPS	286:288	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	1	80	dep	characterized	242:254	arg1	FTIR					257:260	FTIR	257:260	FTIR	257:260	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	1	80	dep	characterized	242:254	arg1	EDS					273:275	EDS	273:275	EDS	273:275	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	1	80	dep	characterized	242:254	arg1	BET					278:280	BET	278:280	BET	278:280	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	1	80	dep	characterized	242:254	arg1	XRD					263:265	XRD	263:265	XRD	263:265	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	9	81	theme	@	1755:1755	arg1	CBC-350					1756:1762	SFM@CBC-350	1752:1762	SFM@CBC-350	1752:1762	Hence, SFM@CBC-350 has the potential of being used as a green, efficient and promising adsorbent in Pb removal from wastewater and soil.					
35764108	3	82	theme	SFM	711:713	arg1	CBC					715:717	SFM@CBC	711:717	SFM@CBC	711:717	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	2	83	theme	Pb2+	416:419	arg1	performance					401:411	highly effective adsorption performance	373:411	highly effective adsorption performance of Pb2+ from wastewater	373:435	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	3	84	theme	Pb2+	703:706	arg1	reaction					767:774	spontaneous and endothermic reaction	739:774	spontaneous and endothermic reaction	739:774	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	3	84	theme	Pb2+	703:706	arg1	absorption					689:698	the absorption	685:698	the absorption of Pb2+ by SFM@CBC and SFM@CBC-350	685:733	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	6	85	theme	removal	1196:1202	arg1	performance					1204:1214	Pb removal performance	1193:1214	Pb removal performance of the modified biochar	1193:1238	In addition, in order to further explore Pb removal performance of the modified biochar, SFM@CBC-350 was used in soil-ryegrass pot systems.					
35764108	7	86	dep	reduced	1348:1354	arg1	increased					1389:1397	increased	1389:1397	increased the biomass and total chlorophyll content	1389:1439	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	7	86	dep	reduced	1348:1354	arg1	reduced					1442:1448	reduced	1442:1448	reduced	1442:1448	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	6	87	theme	pot	1279:1281	arg1	systems					1283:1289	soil-ryegrass pot systems	1265:1289	soil-ryegrass pot systems	1265:1289	In addition, in order to further explore Pb removal performance of the modified biochar, SFM@CBC-350 was used in soil-ryegrass pot systems.					
35764108	3	88	theme	endothermic	755:765	arg1	reaction					767:774	spontaneous and endothermic reaction	739:774	spontaneous and endothermic reaction	739:774	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	3	88	theme	endothermic	755:765	arg1	absorption					689:698	the absorption	685:698	the absorption of Pb2+ by SFM@CBC and SFM@CBC-350	685:733	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	2	89	theme	@	342:342	arg1	CBC					343:345	SFM@CBC	339:345	SFM@CBC	339:345	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	2	90	theme	@	563:563	arg1	149.25 mg g-1					569:581	149.25 mg g-1	569:581	149.25 mg g-1	569:581	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	2	90	theme	@	563:563	arg1	CBC					564:566	FM@CBC	561:566	FM@CBC (149.25 mg g-1)	561:582	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	9	91	from	wastewater	1861:1870	arg1	removal					1848:1854	Pb removal	1845:1854	Pb removal from wastewater and soil	1845:1879	Hence, SFM@CBC-350 has the potential of being used as a green, efficient and promising adsorbent in Pb removal from wastewater and soil.					
35764108	0	92	theme	Fe-Mn/biochar	46:58	arg1	composites					60:69	starch-stablized Fe-Mn/biochar composites	29:69	starch-stablized Fe-Mn/biochar composites	29:69	Synthesis and application of starch-stablized Fe-Mn/biochar composites for the removal of lead from water and soil.					
35764108	7	93	theme	antioxidant	1466:1476	arg1	enzymes					1478:1484	antioxidant enzymes	1466:1484	antioxidant enzymes (CAT, SOD, MDA and POD)	1466:1508	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	7	93	theme	antioxidant	1466:1476	arg1	POD					1505:1507	POD	1505:1507	POD	1505:1507	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	7	93	theme	antioxidant	1466:1476	arg1	MDA					1497:1499	MDA	1497:1499	MDA	1497:1499	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	7	93	theme	antioxidant	1466:1476	arg1	SOD					1492:1494	SOD	1492:1494	SOD	1492:1494	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	7	93	theme	antioxidant	1466:1476	arg1	CAT					1487:1489	CAT	1487:1489	CAT	1487:1489	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	2	94	theme	@	354:354	arg1	CBC-350					355:361	SFM@CBC-350	351:361	SFM@CBC-350	351:361	SFM@CBC and SFM@CBC-350 displayed highly effective adsorption performance of Pb2+ from wastewater with the maximum adsorption capacity of 170.91 mg g-1 and 190.17 mg g-1, respectively, which were much greater than that of FM@CBC (149.25 mg g-1) and CBC (101.10 mg g-1).					
35764108	8	95	theme	ryegrass	1735:1742	arg1	growth					1725:1730	the growth	1721:1730	the growth of ryegrass	1721:1742	Besides, the addition of SFM@CBC-350 could increase the richness and diversity of soil microorganisms, which was beneficial to the growth of ryegrass.					
35764108	6	96	theme	@	1244:1244	arg1	CBC-350					1245:1251	SFM@CBC-350	1241:1251	SFM@CBC-350	1241:1251	In addition, in order to further explore Pb removal performance of the modified biochar, SFM@CBC-350 was used in soil-ryegrass pot systems.					
35764108	7	97	theme	@	1339:1339	arg1	CBC-350					1340:1346	SFM@CBC-350	1336:1346	SFM@CBC-350	1336:1346	Compared with the controls, the addition of SFM@CBC-350 reduced Pb content in soil and ryegrass, increased the biomass and total chlorophyll content, reduced the activity of antioxidant enzymes (CAT, SOD, MDA and POD) and ROS fluorescence intensity of ryegrass, thus alleviating Pb stress of ryegrass.					
35764108	3	98	theme	monolayer	802:810	arg1	chemisorption					812:824	monolayer chemisorption	802:824	monolayer chemisorption	802:824	Studies of adsorption kinetics, isotherms and thermodynamics indicated that the absorption of Pb2+ by SFM@CBC and SFM@CBC-350 was spontaneous and endothermic reaction, and it was controlled by monolayer chemisorption.					
35764108	1	99	theme	@	209:209	arg1	CBC-350					210:216	SFM@CBC-350	206:216	SFM@CBC-350	206:216	Starch-stablized and Fe/Mn bimetals modified biochar derived from corn straw (SFM@CBC and SFM@CBC-350) were firstly prepared, characterized (FTIR, XRD, SEM, EDS, BET and XPS), and applied in Pb removal from water and soil.					
35764108	9	100	from	soil	1876:1879	arg1	removal					1848:1854	Pb removal	1845:1854	Pb removal from wastewater and soil	1845:1879	Hence, SFM@CBC-350 has the potential of being used as a green, efficient and promising adsorbent in Pb removal from wastewater and soil.					
35764108	5	101	theme	@	1102:1102	arg1	CBC-350					1103:1109	SFM@CBC-350	1099:1109	SFM@CBC-350	1099:1109	The reusability test demonstrated that SFM@CBC and SFM@CBC-350 had very good stability and reusability.					
36801785	4	0	theme	natural	619:625	arg1	nanoparticles					654:666	natural green synthesized metallic nanoparticles	619:666	natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications	619:733	This work aimed at the implementation of natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications.					
36801785	11	1	theme	Pd	1486:1487	arg1	NPs					1489:1491	Pd NPs	1486:1491	Pd NPs	1486:1491	The XRD analysis confirmed that doping with Pd NPs does not affect the crystallinity degree of the PVA/Alg hybrid scaffolds.					
36801785	14	2	theme	mechanical	1973:1982	arg1	support					1984:1990	enough mechanical support	1966:1990	enough mechanical support	1966:1990	According to the SEM results, the scaffolds with Pd NPs provided the differentiated grown osteoblast cells with enough mechanical support and stability and the cells had a regular form and were highly dense.					
36801785	9	3	theme	synthesized	1264:1274	arg1	scaffolds					1276:1284	The synthesized scaffolds	1260:1284	The synthesized scaffolds	1260:1284	The synthesized scaffolds were characterized by the oriented lamellar porous structure.					
36801785	4	4	theme	synthesized	633:643	arg1	nanoparticles					654:666	natural green synthesized metallic nanoparticles	619:666	natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications	619:733	This work aimed at the implementation of natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications.					
36801785	11	5	theme	crystallinity	1513:1525	arg1	degree					1527:1532	the crystallinity degree	1509:1532	the crystallinity degree of the PVA/Alg hybrid scaffolds	1509:1564	The XRD analysis confirmed that doping with Pd NPs does not affect the crystallinity degree of the PVA/Alg hybrid scaffolds.					
36801785	2	6	theme	appropriate	303:313	arg1	material					315:322	an appropriate material	300:322	selecting an appropriate material with optimal physical, chemical, and mechanical properties	290:381	However, selecting an appropriate material with optimal physical, chemical, and mechanical properties is considered a great challenge.					
36801785	4	7	theme	dental	715:720	arg1	applications					722:733	dental applications	715:733	dental applications	715:733	This work aimed at the implementation of natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications.					
36801785	2	8	theme	selecting	290:298	arg1	material					315:322	an appropriate material	300:322	selecting an appropriate material with optimal physical, chemical, and mechanical properties	290:381	However, selecting an appropriate material with optimal physical, chemical, and mechanical properties is considered a great challenge.					
36801785	6	9	theme	analysis	952:959	arg1	techniques					961:970	Various characteristic analysis techniques	929:970	Various characteristic analysis techniques	929:970	Various characteristic analysis techniques were used to investigate the synthesized composite scaffold's properties.					
36801785	11	10	theme	XRD	1446:1448	arg1	analysis					1450:1457	The XRD analysis	1442:1457	The XRD analysis	1442:1457	The XRD analysis confirmed that doping with Pd NPs does not affect the crystallinity degree of the PVA/Alg hybrid scaffolds.					
36801785	9	11	theme	oriented	1312:1319	arg1	structure					1337:1345	the oriented lamellar porous structure	1308:1345	the oriented lamellar porous structure	1308:1345	The synthesized scaffolds were characterized by the oriented lamellar porous structure.					
36801785	2	12	theme	physical	337:344	arg1	properties					372:381	optimal physical, chemical, and mechanical properties	329:381	optimal physical, chemical, and mechanical properties	329:381	However, selecting an appropriate material with optimal physical, chemical, and mechanical properties is considered a great challenge.					
36801785	15	13	theme	potential	2255:2263	arg1	option					2265:2270	a potential option	2253:2270	a potential option for treating critical deficiencies of bone	2253:2313	In conclusion, the synthesized composite scaffolds expressed suitable biodegradable, osteoconductive properties, and the ability to construct 3D structures for bone regeneration, making them a potential option for treating critical deficiencies of bone.					
36801785	5	14	theme	hybrid	762:767	arg1	scaffolds					769:777	innovative hybrid scaffolds	751:777	innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs)	751:904	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	9	15	theme	porous	1330:1335	arg1	structure					1337:1345	the oriented lamellar porous structure	1308:1345	the oriented lamellar porous structure	1308:1345	The synthesized scaffolds were characterized by the oriented lamellar porous structure.					
36801785	1	16	theme	attracting	214:223	arg1	scaffolds					200:208	Three-dimensional (3D) scaffolds	177:208	Three-dimensional (3D) scaffolds	177:208	Three-dimensional (3D) scaffolds are attracting great concern for bone tissue engineering applications.					
36801785	1	16	theme	attracting	214:223	arg1	concern					231:237	attracting great concern	214:237	attracting great concern for bone tissue engineering applications	214:278	Three-dimensional (3D) scaffolds are attracting great concern for bone tissue engineering applications.					
36801785	4	17	theme	scaffolds	701:709	arg1	development					676:686	the development	672:686	the development of composite scaffolds for dental applications	672:733	This work aimed at the implementation of natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications.					
36801785	0	18	theme	stem	148:151	arg1	cells					153:157	differentiated human dental pulp stem cells	115:157	differentiated human dental pulp stem cells	115:157	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	7	19	theme	scaffolds	1117:1125	arg1	microstructure					1083:1096	impressive microstructure	1072:1096	impressive microstructure of the synthesized scaffolds dependent on the Pd NPs concentration	1072:1163	The SEM analysis revealed impressive microstructure of the synthesized scaffolds dependent on the Pd NPs concentration.					
36801785	12	20	theme	concentration	1673:1685	arg1	effect					1641:1646	the remarkable effect	1626:1646	the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds	1626:1712	The mechanical properties results (up to 50 MPa) confirmed the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds.					
36801785	13	21	theme	cell	1838:1841	arg1	viability					1843:1851	cell viability	1838:1851	cell viability	1838:1851	The MTT assay results showed that the incorporation of Pd NPs into the nanocomposite scaffolds is necessary for increasing cell viability.					
36801785	7	22	theme	NPs	1147:1149	arg1	concentration					1151:1163	the Pd NPs concentration	1140:1163	the Pd NPs concentration	1140:1163	The SEM analysis revealed impressive microstructure of the synthesized scaffolds dependent on the Pd NPs concentration.					
36801785	6	23	theme	composite	1013:1021	arg1	properties					1034:1043	the synthesized composite scaffold's properties	997:1043	the synthesized composite scaffold's properties	997:1043	Various characteristic analysis techniques were used to investigate the synthesized composite scaffold's properties.					
36801785	14	24	theme	grown	1938:1942	arg1	cells					1955:1959	the differentiated grown osteoblast cells	1919:1959	the differentiated grown osteoblast cells	1919:1959	According to the SEM results, the scaffolds with Pd NPs provided the differentiated grown osteoblast cells with enough mechanical support and stability and the cells had a regular form and were highly dense.					
36801785	15	25	theme	bone	2310:2313	arg1	deficiencies					2294:2305	critical deficiencies	2285:2305	critical deficiencies of bone	2285:2313	In conclusion, the synthesized composite scaffolds expressed suitable biodegradable, osteoconductive properties, and the ability to construct 3D structures for bone regeneration, making them a potential option for treating critical deficiencies of bone.					
36801785	0	26	from	Effect	105:110	arg1	cells					153:157	differentiated human dental pulp stem cells	115:157	differentiated human dental pulp stem cells	115:157	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	5	27	theme	composite	819:827	arg1	scaffolds					769:777	innovative hybrid scaffolds	751:777	innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs)	751:904	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	5	28	theme	Pd	898:899	arg1	nanoparticles					883:895	green palladium nanoparticles	867:895	green palladium nanoparticles (Pd NPs)	867:904	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	5	28	theme	Pd	898:899	arg1	NPs					901:903	Pd NPs	898:903	Pd NPs	898:903	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	12	29	theme	Pd	1651:1652	arg1	doping					1658:1663	Pd NPs doping	1651:1663	Pd NPs doping	1651:1663	The mechanical properties results (up to 50 MPa) confirmed the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds.					
36801785	12	30	theme	NPs	1654:1656	arg1	doping					1658:1663	Pd NPs doping	1651:1663	Pd NPs doping	1651:1663	The mechanical properties results (up to 50 MPa) confirmed the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds.					
36801785	6	31	used	used	977:980	arg2	techniques					961:970	Various characteristic analysis techniques	929:970	Various characteristic analysis techniques	929:970	Various characteristic analysis techniques were used to investigate the synthesized composite scaffold's properties.					
36801785	7	32	theme	SEM	1050:1052	arg1	analysis					1054:1061	The SEM analysis	1046:1061	The SEM analysis	1046:1061	The SEM analysis revealed impressive microstructure of the synthesized scaffolds dependent on the Pd NPs concentration.					
36801785	1	33	theme	Three-dimensional	177:193	arg1	scaffolds					200:208	Three-dimensional (3D) scaffolds	177:208	Three-dimensional (3D) scaffolds	177:208	Three-dimensional (3D) scaffolds are attracting great concern for bone tissue engineering applications.					
36801785	1	33	theme	Three-dimensional	177:193	arg1	concern					231:237	attracting great concern	214:237	attracting great concern for bone tissue engineering applications	214:278	Three-dimensional (3D) scaffolds are attracting great concern for bone tissue engineering applications.					
36801785	5	34	theme	alcohol/alginate	792:807	arg1	composite					819:827	polyvinyl alcohol/alginate (PVA/Alg) composite	782:827	polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs)	782:904	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	12	35	theme	remarkable	1630:1639	arg1	effect					1641:1646	the remarkable effect	1626:1646	the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds	1626:1712	The mechanical properties results (up to 50 MPa) confirmed the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds.					
36801785	11	36	theme	PVA/Alg	1541:1547	arg1	scaffolds					1556:1564	the PVA/Alg hybrid scaffolds	1537:1564	the PVA/Alg hybrid scaffolds	1537:1564	The XRD analysis confirmed that doping with Pd NPs does not affect the crystallinity degree of the PVA/Alg hybrid scaffolds.					
36801785	5	37	theme	PVA/Alg	810:816	arg1	composite					819:827	polyvinyl alcohol/alginate (PVA/Alg) composite	782:827	polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs)	782:904	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	0	38	theme	alginate/	44:52	arg1	palladium					60:68	innovative polyvinyl alcohol/ alginate/ green palladium	14:68	innovative polyvinyl alcohol/ alginate/ green palladium	14:68	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	13	39	theme	NPs	1773:1775	arg1	necessary					1813:1821	necessary	1813:1821	necessary	1813:1821	The MTT assay results showed that the incorporation of Pd NPs into the nanocomposite scaffolds is necessary for increasing cell viability.					
36801785	13	39	theme	NPs	1773:1775	arg1	incorporation					1753:1765	the incorporation	1749:1765	the incorporation of Pd NPs into the nanocomposite scaffolds	1749:1808	The MTT assay results showed that the incorporation of Pd NPs into the nanocomposite scaffolds is necessary for increasing cell viability.					
36801785	5	40	theme	nanoparticles	883:895	arg1	concentrations					849:862	various concentrations	841:862	various concentrations of green palladium nanoparticles (Pd NPs)	841:904	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	14	41	theme	Pd	1903:1904	arg1	NPs					1906:1908	Pd NPs	1903:1908	Pd NPs	1903:1908	According to the SEM results, the scaffolds with Pd NPs provided the differentiated grown osteoblast cells with enough mechanical support and stability and the cells had a regular form and were highly dense.					
36801785	15	42	theme	composite	2093:2101	arg1	scaffolds					2103:2111	the synthesized composite scaffolds	2077:2111	the synthesized composite scaffolds	2077:2111	In conclusion, the synthesized composite scaffolds expressed suitable biodegradable, osteoconductive properties, and the ability to construct 3D structures for bone regeneration, making them a potential option for treating critical deficiencies of bone.					
36801785	3	43	theme	harmful	485:491	arg1	by-products					493:503	harmful by-products	485:503	harmful by-products	485:503	The green synthesis approach is essential to avoid the production of harmful by-products through textured construction, sustainable, and eco-friendly procedures.					
36801785	0	44	theme	innovative	14:23	arg1	palladium					60:68	innovative polyvinyl alcohol/ alginate/ green palladium	14:68	innovative polyvinyl alcohol/ alginate/ green palladium	14:68	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	6	45	theme	characteristic	937:950	arg1	techniques					961:970	Various characteristic analysis techniques	929:970	Various characteristic analysis techniques	929:970	Various characteristic analysis techniques were used to investigate the synthesized composite scaffold's properties.					
36801785	15	46	theme	suitable	2123:2130	arg1	biodegradable					2132:2144	suitable biodegradable	2123:2144	suitable biodegradable	2123:2144	In conclusion, the synthesized composite scaffolds expressed suitable biodegradable, osteoconductive properties, and the ability to construct 3D structures for bone regeneration, making them a potential option for treating critical deficiencies of bone.					
36801785	11	47	theme	scaffolds	1556:1564	arg1	degree					1527:1532	the crystallinity degree	1509:1532	the crystallinity degree of the PVA/Alg hybrid scaffolds	1509:1564	The XRD analysis confirmed that doping with Pd NPs does not affect the crystallinity degree of the PVA/Alg hybrid scaffolds.					
36801785	0	48	theme	alcohol/	35:42	arg1	palladium					60:68	innovative polyvinyl alcohol/ alginate/ green palladium	14:68	innovative polyvinyl alcohol/ alginate/ green palladium	14:68	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	8	49	theme	positive	1192:1199	arg1	effect					1201:1206	the positive effect	1188:1206	the positive effect of Pd NPs doping on the sample stability over time	1188:1257	The results confirmed the positive effect of Pd NPs doping on the sample stability over time.					
36801785	12	50	theme	doping	1658:1663	arg1	effect					1641:1646	the remarkable effect	1626:1646	the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds	1626:1712	The mechanical properties results (up to 50 MPa) confirmed the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds.					
36801785	3	51	theme	green	420:424	arg1	essential					448:456	essential	448:456	essential	448:456	The green synthesis approach is essential to avoid the production of harmful by-products through textured construction, sustainable, and eco-friendly procedures.					
36801785	3	51	theme	green	420:424	arg1	approach					436:443	The green synthesis approach	416:443	The green synthesis approach	416:443	The green synthesis approach is essential to avoid the production of harmful by-products through textured construction, sustainable, and eco-friendly procedures.					
36801785	3	52	theme	construction	522:533	arg1	procedures					566:575	textured construction, sustainable, and eco-friendly procedures	513:575	textured construction, sustainable, and eco-friendly procedures	513:575	The green synthesis approach is essential to avoid the production of harmful by-products through textured construction, sustainable, and eco-friendly procedures.					
36801785	5	53	theme	green	867:871	arg1	nanoparticles					883:895	green palladium nanoparticles	867:895	green palladium nanoparticles (Pd NPs)	867:904	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	5	53	theme	green	867:871	arg1	NPs					901:903	Pd NPs	898:903	Pd NPs	898:903	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	14	54	theme	SEM	1871:1873	arg1	results					1875:1881	the SEM results	1867:1881	the SEM results	1867:1881	According to the SEM results, the scaffolds with Pd NPs provided the differentiated grown osteoblast cells with enough mechanical support and stability and the cells had a regular form and were highly dense.					
36801785	12	55	theme	properties	1582:1591	arg1	results					1593:1599	The mechanical properties results	1567:1599	The mechanical properties results (up to 50 MPa)	1567:1614	The mechanical properties results (up to 50 MPa) confirmed the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds.					
36801785	13	56	theme	assay	1723:1727	arg1	results					1729:1735	The MTT assay results	1715:1735	The MTT assay results	1715:1735	The MTT assay results showed that the incorporation of Pd NPs into the nanocomposite scaffolds is necessary for increasing cell viability.					
36801785	15	57	theme	bone	2222:2225	arg1	regeneration					2227:2238	bone regeneration	2222:2238	bone regeneration	2222:2238	In conclusion, the synthesized composite scaffolds expressed suitable biodegradable, osteoconductive properties, and the ability to construct 3D structures for bone regeneration, making them a potential option for treating critical deficiencies of bone.					
36801785	8	58	theme	NPs	1214:1216	arg1	doping					1218:1223	Pd NPs doping	1211:1223	Pd NPs doping	1211:1223	The results confirmed the positive effect of Pd NPs doping on the sample stability over time.					
36801785	14	59	theme	regular	2026:2032	arg1	form					2034:2037	a regular form	2024:2037	a regular form	2024:2037	According to the SEM results, the scaffolds with Pd NPs provided the differentiated grown osteoblast cells with enough mechanical support and stability and the cells had a regular form and were highly dense.					
36801785	4	60	theme	metallic	645:652	arg1	nanoparticles					654:666	natural green synthesized metallic nanoparticles	619:666	natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications	619:733	This work aimed at the implementation of natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications.					
36801785	1	61	theme	tissue	248:253	arg1	applications					267:278	bone tissue engineering applications	243:278	bone tissue engineering applications	243:278	Three-dimensional (3D) scaffolds are attracting great concern for bone tissue engineering applications.					
36801785	6	62	theme	synthesized	1001:1011	arg1	properties					1034:1043	the synthesized composite scaffold's properties	997:1043	the synthesized composite scaffold's properties	997:1043	Various characteristic analysis techniques were used to investigate the synthesized composite scaffold's properties.					
36801785	3	63	theme	sustainable	536:546	arg1	procedures					566:575	textured construction, sustainable, and eco-friendly procedures	513:575	textured construction, sustainable, and eco-friendly procedures	513:575	The green synthesis approach is essential to avoid the production of harmful by-products through textured construction, sustainable, and eco-friendly procedures.					
36801785	8	64	theme	sample	1232:1237	arg1	stability					1239:1247	the sample stability	1228:1247	the sample stability over time	1228:1257	The results confirmed the positive effect of Pd NPs doping on the sample stability over time.					
36801785	4	65	theme	green	627:631	arg1	nanoparticles					654:666	natural green synthesized metallic nanoparticles	619:666	natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications	619:733	This work aimed at the implementation of natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications.					
36801785	2	66	theme	mechanical	361:370	arg1	properties					372:381	optimal physical, chemical, and mechanical properties	329:381	optimal physical, chemical, and mechanical properties	329:381	However, selecting an appropriate material with optimal physical, chemical, and mechanical properties is considered a great challenge.					
36801785	14	67	with	scaffolds	1888:1896	arg1	NPs					1906:1908	Pd NPs	1903:1908	Pd NPs	1903:1908	According to the SEM results, the scaffolds with Pd NPs provided the differentiated grown osteoblast cells with enough mechanical support and stability and the cells had a regular form and were highly dense.					
36801785	0	68	theme	green	54:58	arg1	palladium					60:68	innovative polyvinyl alcohol/ alginate/ green palladium	14:68	innovative polyvinyl alcohol/ alginate/ green palladium	14:68	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	12	69	theme	developed	1694:1702	arg1	scaffolds					1704:1712	the developed scaffolds	1690:1712	the developed scaffolds	1690:1712	The mechanical properties results (up to 50 MPa) confirmed the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds.					
36801785	0	70	theme	pulp	143:146	arg1	cells					153:157	differentiated human dental pulp stem cells	115:157	differentiated human dental pulp stem cells	115:157	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	9	71	theme	lamellar	1321:1328	arg1	structure					1337:1345	the oriented lamellar porous structure	1308:1345	the oriented lamellar porous structure	1308:1345	The synthesized scaffolds were characterized by the oriented lamellar porous structure.					
36801785	2	72	theme	optimal	329:335	arg1	properties					372:381	optimal physical, chemical, and mechanical properties	329:381	optimal physical, chemical, and mechanical properties	329:381	However, selecting an appropriate material with optimal physical, chemical, and mechanical properties is considered a great challenge.					
36801785	15	73	theme	critical	2285:2292	arg1	deficiencies					2294:2305	critical deficiencies	2285:2305	critical deficiencies of bone	2285:2313	In conclusion, the synthesized composite scaffolds expressed suitable biodegradable, osteoconductive properties, and the ability to construct 3D structures for bone regeneration, making them a potential option for treating critical deficiencies of bone.					
36801785	1	74	theme	great	225:229	arg1	scaffolds					200:208	Three-dimensional (3D) scaffolds	177:208	Three-dimensional (3D) scaffolds	177:208	Three-dimensional (3D) scaffolds are attracting great concern for bone tissue engineering applications.					
36801785	1	74	theme	great	225:229	arg1	concern					231:237	attracting great concern	214:237	attracting great concern for bone tissue engineering applications	214:278	Three-dimensional (3D) scaffolds are attracting great concern for bone tissue engineering applications.					
36801785	4	75	theme	composite	691:699	arg1	scaffolds					701:709	composite scaffolds	691:709	composite scaffolds for dental applications	691:733	This work aimed at the implementation of natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications.					
36801785	7	76	theme	Pd	1144:1145	arg1	concentration					1151:1163	the Pd NPs concentration	1140:1163	the Pd NPs concentration	1140:1163	The SEM analysis revealed impressive microstructure of the synthesized scaffolds dependent on the Pd NPs concentration.					
36801785	7	77	theme	dependent	1127:1135	arg1	scaffolds					1117:1125	the synthesized scaffolds	1101:1125	the synthesized scaffolds dependent on the Pd NPs concentration	1101:1163	The SEM analysis revealed impressive microstructure of the synthesized scaffolds dependent on the Pd NPs concentration.					
36801785	5	78	theme	innovative	751:760	arg1	scaffolds					769:777	innovative hybrid scaffolds	751:777	innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs)	751:904	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	7	79	theme	synthesized	1105:1115	arg1	scaffolds					1117:1125	the synthesized scaffolds	1101:1125	the synthesized scaffolds dependent on the Pd NPs concentration	1101:1163	The SEM analysis revealed impressive microstructure of the synthesized scaffolds dependent on the Pd NPs concentration.					
36801785	14	80	theme	osteoblast	1944:1953	arg1	cells					1955:1959	the differentiated grown osteoblast cells	1919:1959	the differentiated grown osteoblast cells	1919:1959	According to the SEM results, the scaffolds with Pd NPs provided the differentiated grown osteoblast cells with enough mechanical support and stability and the cells had a regular form and were highly dense.					
36801785	8	81	from	effect	1201:1206	arg1	stability					1239:1247	the sample stability	1228:1247	the sample stability over time	1228:1257	The results confirmed the positive effect of Pd NPs doping on the sample stability over time.					
36801785	12	82	dep	results	1593:1599	arg1	up					1602:1603	up	1602:1603	up to 50 MPa	1602:1613	The mechanical properties results (up to 50 MPa) confirmed the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds.					
36801785	0	83	theme	composite	84:92	arg1	scaffolds					94:102	composite scaffolds	84:102	composite scaffolds	84:102	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	14	84	theme	differentiated	1923:1936	arg1	cells					1955:1959	the differentiated grown osteoblast cells	1919:1959	the differentiated grown osteoblast cells	1919:1959	According to the SEM results, the scaffolds with Pd NPs provided the differentiated grown osteoblast cells with enough mechanical support and stability and the cells had a regular form and were highly dense.					
36801785	2	85	with	material	315:322	arg1	properties					372:381	optimal physical, chemical, and mechanical properties	329:381	optimal physical, chemical, and mechanical properties	329:381	However, selecting an appropriate material with optimal physical, chemical, and mechanical properties is considered a great challenge.					
36801785	10	86	theme	shape	1374:1378	arg1	stability					1380:1388	the shape stability	1370:1388	the shape stability	1370:1388	The results confirmed the shape stability, without pores breakdown during the drying process.					
36801785	7	87	theme	impressive	1072:1081	arg1	microstructure					1083:1096	impressive microstructure	1072:1096	impressive microstructure of the synthesized scaffolds dependent on the Pd NPs concentration	1072:1163	The SEM analysis revealed impressive microstructure of the synthesized scaffolds dependent on the Pd NPs concentration.					
36801785	0	88	theme	dental	136:141	arg1	cells					153:157	differentiated human dental pulp stem cells	115:157	differentiated human dental pulp stem cells	115:157	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	13	89	theme	nanocomposite	1786:1798	arg1	scaffolds					1800:1808	the nanocomposite scaffolds	1782:1808	the nanocomposite scaffolds	1782:1808	The MTT assay results showed that the incorporation of Pd NPs into the nanocomposite scaffolds is necessary for increasing cell viability.					
36801785	5	90	theme	polyvinyl	782:790	arg1	composite					819:827	polyvinyl alcohol/alginate (PVA/Alg) composite	782:827	polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs)	782:904	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	10	91	theme	pores	1399:1403	arg1	breakdown					1405:1413	pores breakdown	1399:1413	pores breakdown	1399:1413	The results confirmed the shape stability, without pores breakdown during the drying process.					
36801785	2	92	theme	chemical	347:354	arg1	properties					372:381	optimal physical, chemical, and mechanical properties	329:381	optimal physical, chemical, and mechanical properties	329:381	However, selecting an appropriate material with optimal physical, chemical, and mechanical properties is considered a great challenge.					
36801785	0	93	theme	human	130:134	arg1	cells					153:157	differentiated human dental pulp stem cells	115:157	differentiated human dental pulp stem cells	115:157	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	12	94	from	effect	1641:1646	arg1	scaffolds					1704:1712	the developed scaffolds	1690:1712	the developed scaffolds	1690:1712	The mechanical properties results (up to 50 MPa) confirmed the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds.					
36801785	15	95	theme	osteoconductive	2147:2161	arg1	properties					2163:2172	osteoconductive properties	2147:2172	osteoconductive properties	2147:2172	In conclusion, the synthesized composite scaffolds expressed suitable biodegradable, osteoconductive properties, and the ability to construct 3D structures for bone regeneration, making them a potential option for treating critical deficiencies of bone.					
36801785	13	96	theme	Pd	1770:1771	arg1	NPs					1773:1775	Pd NPs	1770:1775	Pd NPs	1770:1775	The MTT assay results showed that the incorporation of Pd NPs into the nanocomposite scaffolds is necessary for increasing cell viability.					
36801785	11	97	with	doping	1474:1479	arg1	NPs					1489:1491	Pd NPs	1486:1491	Pd NPs	1486:1491	The XRD analysis confirmed that doping with Pd NPs does not affect the crystallinity degree of the PVA/Alg hybrid scaffolds.					
36801785	5	98	theme	palladium	873:881	arg1	nanoparticles					883:895	green palladium nanoparticles	867:895	green palladium nanoparticles (Pd NPs)	867:904	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	5	98	theme	palladium	873:881	arg1	NPs					901:903	Pd NPs	898:903	Pd NPs	898:903	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	1	99	dep	Three-dimensional	177:193	arg1	3D					196:197	3D	196:197	3D	196:197	Three-dimensional (3D) scaffolds are attracting great concern for bone tissue engineering applications.					
36801785	10	100	theme	drying	1426:1431	arg1	process					1433:1439	the drying process	1422:1439	the drying process	1422:1439	The results confirmed the shape stability, without pores breakdown during the drying process.					
36801785	2	101	theme	great	399:403	arg1	challenge					405:413	a great challenge	397:413	a great challenge	397:413	However, selecting an appropriate material with optimal physical, chemical, and mechanical properties is considered a great challenge.					
36801785	0	102	theme	polyvinyl	25:33	arg1	palladium					60:68	innovative polyvinyl alcohol/ alginate/ green palladium	14:68	innovative polyvinyl alcohol/ alginate/ green palladium	14:68	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	11	103	theme	hybrid	1549:1554	arg1	scaffolds					1556:1564	the PVA/Alg hybrid scaffolds	1537:1564	the PVA/Alg hybrid scaffolds	1537:1564	The XRD analysis confirmed that doping with Pd NPs does not affect the crystallinity degree of the PVA/Alg hybrid scaffolds.					
36801785	3	104	theme	by-products	493:503	arg1	production					471:480	the production	467:480	the production of harmful by-products	467:503	The green synthesis approach is essential to avoid the production of harmful by-products through textured construction, sustainable, and eco-friendly procedures.					
36801785	5	105	theme	various	841:847	arg1	concentrations					849:862	various concentrations	841:862	various concentrations of green palladium nanoparticles (Pd NPs)	841:904	In this study, innovative hybrid scaffolds of polyvinyl alcohol/alginate (PVA/Alg) composite loaded with various concentrations of green palladium nanoparticles (Pd NPs) have been synthesized.					
36801785	6	106	theme	Various	929:935	arg1	techniques					961:970	Various characteristic analysis techniques	929:970	Various characteristic analysis techniques	929:970	Various characteristic analysis techniques were used to investigate the synthesized composite scaffold's properties.					
36801785	15	107	theme	synthesized	2081:2091	arg1	scaffolds					2103:2111	the synthesized composite scaffolds	2077:2111	the synthesized composite scaffolds	2077:2111	In conclusion, the synthesized composite scaffolds expressed suitable biodegradable, osteoconductive properties, and the ability to construct 3D structures for bone regeneration, making them a potential option for treating critical deficiencies of bone.					
36801785	0	108	theme	differentiated	115:128	arg1	cells					153:157	differentiated human dental pulp stem cells	115:157	differentiated human dental pulp stem cells	115:157	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	0	109	theme	palladium	60:68	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium	0:68	Evaluation of innovative polyvinyl alcohol/ alginate/ green palladium nanoparticles composite scaffolds: Effect on differentiated human dental pulp stem cells into osteoblasts.					
36801785	8	110	theme	Pd	1211:1212	arg1	doping					1218:1223	Pd NPs doping	1211:1223	Pd NPs doping	1211:1223	The results confirmed the positive effect of Pd NPs doping on the sample stability over time.					
36801785	15	111	theme	3D	2204:2205	arg1	structures					2207:2216	3D structures	2204:2216	3D structures for bone regeneration	2204:2238	In conclusion, the synthesized composite scaffolds expressed suitable biodegradable, osteoconductive properties, and the ability to construct 3D structures for bone regeneration, making them a potential option for treating critical deficiencies of bone.					
36801785	3	112	theme	synthesis	426:434	arg1	essential					448:456	essential	448:456	essential	448:456	The green synthesis approach is essential to avoid the production of harmful by-products through textured construction, sustainable, and eco-friendly procedures.					
36801785	3	112	theme	synthesis	426:434	arg1	approach					436:443	The green synthesis approach	416:443	The green synthesis approach	416:443	The green synthesis approach is essential to avoid the production of harmful by-products through textured construction, sustainable, and eco-friendly procedures.					
36801785	13	113	theme	MTT	1719:1721	arg1	results					1729:1735	The MTT assay results	1715:1735	The MTT assay results	1715:1735	The MTT assay results showed that the incorporation of Pd NPs into the nanocomposite scaffolds is necessary for increasing cell viability.					
36801785	14	114	contain	had	2020:2022	arg1	dense					2055:2059	dense	2055:2059	dense	2055:2059	According to the SEM results, the scaffolds with Pd NPs provided the differentiated grown osteoblast cells with enough mechanical support and stability and the cells had a regular form and were highly dense.					
36801785	14	114	contain	had	2020:2022	arg2	form					2034:2037	a regular form	2024:2037	a regular form	2024:2037	According to the SEM results, the scaffolds with Pd NPs provided the differentiated grown osteoblast cells with enough mechanical support and stability and the cells had a regular form and were highly dense.					
36801785	14	114	contain	had	2020:2022	arg1	cells					2014:2018	the cells	2010:2018	the cells	2010:2018	According to the SEM results, the scaffolds with Pd NPs provided the differentiated grown osteoblast cells with enough mechanical support and stability and the cells had a regular form and were highly dense.					
36801785	8	115	theme	doping	1218:1223	arg1	effect					1201:1206	the positive effect	1188:1206	the positive effect of Pd NPs doping on the sample stability over time	1188:1257	The results confirmed the positive effect of Pd NPs doping on the sample stability over time.					
36801785	12	116	theme	mechanical	1571:1580	arg1	properties					1582:1591	The mechanical properties	1567:1591	The mechanical properties results (up to 50 MPa)	1567:1614	The mechanical properties results (up to 50 MPa) confirmed the remarkable effect of Pd NPs doping and its concentration on the developed scaffolds.					
36801785	4	117	theme	nanoparticles	654:666	arg1	implementation					601:614	the implementation	597:614	the implementation of natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications	597:733	This work aimed at the implementation of natural green synthesized metallic nanoparticles for the development of composite scaffolds for dental applications.					
36801785	3	118	theme	textured	513:520	arg1	procedures					566:575	textured construction, sustainable, and eco-friendly procedures	513:575	textured construction, sustainable, and eco-friendly procedures	513:575	The green synthesis approach is essential to avoid the production of harmful by-products through textured construction, sustainable, and eco-friendly procedures.					
36801785	1	119	theme	bone	243:246	arg1	applications					267:278	bone tissue engineering applications	243:278	bone tissue engineering applications	243:278	Three-dimensional (3D) scaffolds are attracting great concern for bone tissue engineering applications.					
36801785	3	120	theme	eco-friendly	553:564	arg1	procedures					566:575	textured construction, sustainable, and eco-friendly procedures	513:575	textured construction, sustainable, and eco-friendly procedures	513:575	The green synthesis approach is essential to avoid the production of harmful by-products through textured construction, sustainable, and eco-friendly procedures.					
36801785	1	121	theme	engineering	255:265	arg1	applications					267:278	bone tissue engineering applications	243:278	bone tissue engineering applications	243:278	Three-dimensional (3D) scaffolds are attracting great concern for bone tissue engineering applications.					
37163876	8	0	theme	hydrogel-transplanted	1200:1220	arg1	photoreceptors					1222:1235	the hydrogel-transplanted photoreceptors	1196:1235	the hydrogel-transplanted photoreceptors	1196:1235	Nanotube-like donor cell protrusions were significantly reduced in the hydrogel-transplanted photoreceptors compared to the saline control group, which suggests that HAMC limits the contact required to the host retina for transfer.					
37163876	4	1	from	effects	630:636	arg1	transfer					650:657	material transfer	641:657	material transfer	641:657	Biomaterials, such as hyaluronan-based hydrogels, can support donor cell survival but have not been evaluated for effects on material transfer.					
37163876	6	2	theme	hyaluronan	844:853	arg1	hydrogel					882:889	a hyaluronan and methylcellulose (HAMC) hydrogel	842:889	a hyaluronan and methylcellulose (HAMC) hydrogel	842:889	Photoreceptors delivered to the subretinal space in mice in a hyaluronan and methylcellulose (HAMC) hydrogel showed reduced material transfer.					
37163876	5	3	dep	achieve	715:721	arg1	occurred					772:779	occurred	772:779	occurred	772:779	With increased survival, we hypothesized that we would achieve greater material transfer; however, the opposite occurred.					
37163876	4	4	theme	hyaluronan-based	538:553	arg1	hydrogels					555:563	hyaluronan-based hydrogels	538:563	hyaluronan-based hydrogels	538:563	Biomaterials, such as hyaluronan-based hydrogels, can support donor cell survival but have not been evaluated for effects on material transfer.					
37163876	8	5	theme	saline	1253:1258	arg1	group					1268:1272	the saline control group	1249:1272	the saline control group	1249:1272	Nanotube-like donor cell protrusions were significantly reduced in the hydrogel-transplanted photoreceptors compared to the saline control group, which suggests that HAMC limits the contact required to the host retina for transfer.					
37163876	2	6	theme	cytoplasmic	304:314	arg1	components					316:325	cytoplasmic components	304:325	cytoplasmic components	304:325	Transplanted photoreceptors, which mediate light sensitivity in the retina, transfer cytoplasmic components to host photoreceptors instead of integrating into the tissue.					
37163876	3	7	theme	Donor	390:394	arg1	transfer					410:417	Donor cell material transfer	390:417	Donor cell material transfer	390:417	Donor cell material transfer could, therefore, function as a protein augmentation strategy to restore photoreceptor function.					
37163876	3	7	theme	Donor	390:394	arg1	strategy					472:479	a protein augmentation strategy	449:479	a protein augmentation strategy to restore photoreceptor function	449:513	Donor cell material transfer could, therefore, function as a protein augmentation strategy to restore photoreceptor function.					
37163876	3	8	theme	photoreceptor	492:504	arg1	function					506:513	photoreceptor function	492:513	photoreceptor function	492:513	Donor cell material transfer could, therefore, function as a protein augmentation strategy to restore photoreceptor function.					
37163876	3	9	theme	protein	451:457	arg1	transfer					410:417	Donor cell material transfer	390:417	Donor cell material transfer	390:417	Donor cell material transfer could, therefore, function as a protein augmentation strategy to restore photoreceptor function.					
37163876	3	9	theme	protein	451:457	arg1	strategy					472:479	a protein augmentation strategy	449:479	a protein augmentation strategy to restore photoreceptor function	449:513	Donor cell material transfer could, therefore, function as a protein augmentation strategy to restore photoreceptor function.					
37163876	7	10	theme	cytosolic	972:980	arg1	transfer					990:997	cytosolic protein transfer	972:997	cytosolic protein transfer	972:997	We examined mitochondria transfer in vitro and cytosolic protein transfer in vivo and demonstrate that HAMC significantly reduced transfer in both contexts, which we ascribe to reduced cell-cell contact.					
37163876	1	11	theme	tremendous	88:97	arg1	promise					99:105	tremendous promise	88:105	tremendous promise for vision restoration	88:128	Cell therapy holds tremendous promise for vision restoration; yet donor cell survival and integration continue to limit efficacy of these strategies.					
37163876	9	12	theme	transplanted	1415:1426	arg1	cells					1434:1438	transplanted donor cells	1415:1438	transplanted donor cells	1415:1438	Thus, HAMC can be used to manipulate the behaviour of transplanted donor cells in cell therapy strategies.					
37163876	3	13	theme	augmentation	459:470	arg1	transfer					410:417	Donor cell material transfer	390:417	Donor cell material transfer	390:417	Donor cell material transfer could, therefore, function as a protein augmentation strategy to restore photoreceptor function.					
37163876	3	13	theme	augmentation	459:470	arg1	strategy					472:479	a protein augmentation strategy	449:479	a protein augmentation strategy to restore photoreceptor function	449:513	Donor cell material transfer could, therefore, function as a protein augmentation strategy to restore photoreceptor function.					
37163876	0	14	theme	assisted	9:16	arg1	delivery					32:39	Hydrogel assisted photoreceptor delivery	0:39	Hydrogel assisted photoreceptor delivery	0:39	Hydrogel assisted photoreceptor delivery inhibits material transfer.					
37163876	6	15	theme	material	906:913	arg1	transfer					915:922	reduced material transfer	898:922	reduced material transfer	898:922	Photoreceptors delivered to the subretinal space in mice in a hyaluronan and methylcellulose (HAMC) hydrogel showed reduced material transfer.					
37163876	5	16	theme	increased	665:673	arg1	survival					675:682	increased survival	665:682	increased survival	665:682	With increased survival, we hypothesized that we would achieve greater material transfer; however, the opposite occurred.					
37163876	7	17	theme	protein	982:988	arg1	transfer					990:997	cytosolic protein transfer	972:997	cytosolic protein transfer	972:997	We examined mitochondria transfer in vitro and cytosolic protein transfer in vivo and demonstrate that HAMC significantly reduced transfer in both contexts, which we ascribe to reduced cell-cell contact.					
37163876	8	18	theme	cell	1149:1152	arg1	protrusions					1154:1164	Nanotube-like donor cell protrusions	1129:1164	Nanotube-like donor cell protrusions	1129:1164	Nanotube-like donor cell protrusions were significantly reduced in the hydrogel-transplanted photoreceptors compared to the saline control group, which suggests that HAMC limits the contact required to the host retina for transfer.					
37163876	0	19	theme	Hydrogel	0:7	arg1	delivery					32:39	Hydrogel assisted photoreceptor delivery	0:39	Hydrogel assisted photoreceptor delivery	0:39	Hydrogel assisted photoreceptor delivery inhibits material transfer.					
37163876	6	20	theme	subretinal	814:823	arg1	space					825:829	the subretinal space	810:829	the subretinal space in mice in a hyaluronan and methylcellulose (HAMC) hydrogel	810:889	Photoreceptors delivered to the subretinal space in mice in a hyaluronan and methylcellulose (HAMC) hydrogel showed reduced material transfer.					
37163876	2	21	theme	host	330:333	arg1	photoreceptors					335:348	host photoreceptors	330:348	host photoreceptors instead of integrating into the tissue	330:387	Transplanted photoreceptors, which mediate light sensitivity in the retina, transfer cytoplasmic components to host photoreceptors instead of integrating into the tissue.					
37163876	9	22	theme	therapy	1448:1454	arg1	strategies					1456:1465	cell therapy strategies	1443:1465	cell therapy strategies	1443:1465	Thus, HAMC can be used to manipulate the behaviour of transplanted donor cells in cell therapy strategies.					
37163876	3	23	theme	cell	396:399	arg1	transfer					410:417	Donor cell material transfer	390:417	Donor cell material transfer	390:417	Donor cell material transfer could, therefore, function as a protein augmentation strategy to restore photoreceptor function.					
37163876	3	23	theme	cell	396:399	arg1	strategy					472:479	a protein augmentation strategy	449:479	a protein augmentation strategy to restore photoreceptor function	449:513	Donor cell material transfer could, therefore, function as a protein augmentation strategy to restore photoreceptor function.					
37163876	4	24	theme	cell	584:587	arg1	survival					589:596	donor cell survival	578:596	donor cell survival	578:596	Biomaterials, such as hyaluronan-based hydrogels, can support donor cell survival but have not been evaluated for effects on material transfer.					
37163876	5	25	theme	material	731:738	arg1	transfer					740:747	greater material transfer	723:747	greater material transfer	723:747	With increased survival, we hypothesized that we would achieve greater material transfer; however, the opposite occurred.					
37163876	1	26	theme	vision	111:116	arg1	restoration					118:128	vision restoration	111:128	vision restoration	111:128	Cell therapy holds tremendous promise for vision restoration; yet donor cell survival and integration continue to limit efficacy of these strategies.					
37163876	3	27	theme	material	401:408	arg1	transfer					410:417	Donor cell material transfer	390:417	Donor cell material transfer	390:417	Donor cell material transfer could, therefore, function as a protein augmentation strategy to restore photoreceptor function.					
37163876	3	27	theme	material	401:408	arg1	strategy					472:479	a protein augmentation strategy	449:479	a protein augmentation strategy to restore photoreceptor function	449:513	Donor cell material transfer could, therefore, function as a protein augmentation strategy to restore photoreceptor function.					
37163876	7	28	theme	cell-cell	1110:1118	arg1	contact					1120:1126	reduced cell-cell contact	1102:1126	reduced cell-cell contact	1102:1126	We examined mitochondria transfer in vitro and cytosolic protein transfer in vivo and demonstrate that HAMC significantly reduced transfer in both contexts, which we ascribe to reduced cell-cell contact.					
37163876	6	29	theme	reduced	898:904	arg1	transfer					915:922	reduced material transfer	898:922	reduced material transfer	898:922	Photoreceptors delivered to the subretinal space in mice in a hyaluronan and methylcellulose (HAMC) hydrogel showed reduced material transfer.					
37163876	1	30	theme	strategies	207:216	arg1	efficacy					189:196	efficacy	189:196	efficacy of these strategies	189:216	Cell therapy holds tremendous promise for vision restoration; yet donor cell survival and integration continue to limit efficacy of these strategies.					
37163876	0	31	theme	material	50:57	arg1	transfer					59:66	material transfer	50:66	material transfer	50:66	Hydrogel assisted photoreceptor delivery inhibits material transfer.					
37163876	8	32	theme	donor	1143:1147	arg1	protrusions					1154:1164	Nanotube-like donor cell protrusions	1129:1164	Nanotube-like donor cell protrusions	1129:1164	Nanotube-like donor cell protrusions were significantly reduced in the hydrogel-transplanted photoreceptors compared to the saline control group, which suggests that HAMC limits the contact required to the host retina for transfer.					
37163876	7	33	theme	mitochondria	937:948	arg1	transfer					950:957	mitochondria transfer	937:957	mitochondria transfer in vitro	937:966	We examined mitochondria transfer in vitro and cytosolic protein transfer in vivo and demonstrate that HAMC significantly reduced transfer in both contexts, which we ascribe to reduced cell-cell contact.					
37163876	0	34	theme	photoreceptor	18:30	arg1	delivery					32:39	Hydrogel assisted photoreceptor delivery	0:39	Hydrogel assisted photoreceptor delivery	0:39	Hydrogel assisted photoreceptor delivery inhibits material transfer.					
37163876	8	35	theme	Nanotube-like	1129:1141	arg1	protrusions					1154:1164	Nanotube-like donor cell protrusions	1129:1164	Nanotube-like donor cell protrusions	1129:1164	Nanotube-like donor cell protrusions were significantly reduced in the hydrogel-transplanted photoreceptors compared to the saline control group, which suggests that HAMC limits the contact required to the host retina for transfer.					
37163876	6	36	from	space	825:829	arg1	mice					834:837	mice	834:837	mice in a hyaluronan and methylcellulose (HAMC) hydrogel	834:889	Photoreceptors delivered to the subretinal space in mice in a hyaluronan and methylcellulose (HAMC) hydrogel showed reduced material transfer.					
37163876	9	37	used	used	1379:1382	arg2	HAMC					1367:1370	HAMC	1367:1370	HAMC	1367:1370	Thus, HAMC can be used to manipulate the behaviour of transplanted donor cells in cell therapy strategies.					
37163876	4	38	theme	donor	578:582	arg1	survival					589:596	donor cell survival	578:596	donor cell survival	578:596	Biomaterials, such as hyaluronan-based hydrogels, can support donor cell survival but have not been evaluated for effects on material transfer.					
37163876	7	39	theme	reduced	1102:1108	arg1	contact					1120:1126	reduced cell-cell contact	1102:1126	reduced cell-cell contact	1102:1126	We examined mitochondria transfer in vitro and cytosolic protein transfer in vivo and demonstrate that HAMC significantly reduced transfer in both contexts, which we ascribe to reduced cell-cell contact.					
37163876	2	40	theme	Transplanted	219:230	arg1	photoreceptors					232:245	Transplanted photoreceptors	219:245	Transplanted photoreceptors	219:245	Transplanted photoreceptors, which mediate light sensitivity in the retina, transfer cytoplasmic components to host photoreceptors instead of integrating into the tissue.					
37163876	9	41	from	behaviour	1402:1410	arg1	strategies					1456:1465	cell therapy strategies	1443:1465	cell therapy strategies	1443:1465	Thus, HAMC can be used to manipulate the behaviour of transplanted donor cells in cell therapy strategies.					
37163876	9	42	theme	donor	1428:1432	arg1	cells					1434:1438	transplanted donor cells	1415:1438	transplanted donor cells	1415:1438	Thus, HAMC can be used to manipulate the behaviour of transplanted donor cells in cell therapy strategies.					
37163876	1	43	theme	donor	135:139	arg1	survival					146:153	donor cell survival	135:153	donor cell survival	135:153	Cell therapy holds tremendous promise for vision restoration; yet donor cell survival and integration continue to limit efficacy of these strategies.					
37163876	9	44	theme	cells	1434:1438	arg1	behaviour					1402:1410	the behaviour	1398:1410	the behaviour of transplanted donor cells in cell therapy strategies	1398:1465	Thus, HAMC can be used to manipulate the behaviour of transplanted donor cells in cell therapy strategies.					
37163876	5	45	theme	greater	723:729	arg1	transfer					740:747	greater material transfer	723:747	greater material transfer	723:747	With increased survival, we hypothesized that we would achieve greater material transfer; however, the opposite occurred.					
37163876	6	46	theme	methylcellulose	859:873	arg1	hydrogel					882:889	a hyaluronan and methylcellulose (HAMC) hydrogel	842:889	a hyaluronan and methylcellulose (HAMC) hydrogel	842:889	Photoreceptors delivered to the subretinal space in mice in a hyaluronan and methylcellulose (HAMC) hydrogel showed reduced material transfer.					
37163876	1	47	theme	Cell	69:72	arg1	therapy					74:80	Cell therapy	69:80	Cell therapy	69:80	Cell therapy holds tremendous promise for vision restoration; yet donor cell survival and integration continue to limit efficacy of these strategies.					
37163876	1	48	theme	cell	141:144	arg1	survival					146:153	donor cell survival	135:153	donor cell survival	135:153	Cell therapy holds tremendous promise for vision restoration; yet donor cell survival and integration continue to limit efficacy of these strategies.					
37163876	8	49	theme	host	1335:1338	arg1	retina					1340:1345	the host retina	1331:1345	the host retina	1331:1345	Nanotube-like donor cell protrusions were significantly reduced in the hydrogel-transplanted photoreceptors compared to the saline control group, which suggests that HAMC limits the contact required to the host retina for transfer.					
37163876	6	50	from	mice	834:837	arg1	hydrogel					882:889	a hyaluronan and methylcellulose (HAMC) hydrogel	842:889	a hyaluronan and methylcellulose (HAMC) hydrogel	842:889	Photoreceptors delivered to the subretinal space in mice in a hyaluronan and methylcellulose (HAMC) hydrogel showed reduced material transfer.					
37163876	9	51	theme	cell	1443:1446	arg1	strategies					1456:1465	cell therapy strategies	1443:1465	cell therapy strategies	1443:1465	Thus, HAMC can be used to manipulate the behaviour of transplanted donor cells in cell therapy strategies.					
37163876	2	52	theme	light	262:266	arg1	sensitivity					268:278	light sensitivity	262:278	light sensitivity	262:278	Transplanted photoreceptors, which mediate light sensitivity in the retina, transfer cytoplasmic components to host photoreceptors instead of integrating into the tissue.					
37163876	8	53	theme	control	1260:1266	arg1	group					1268:1272	the saline control group	1249:1272	the saline control group	1249:1272	Nanotube-like donor cell protrusions were significantly reduced in the hydrogel-transplanted photoreceptors compared to the saline control group, which suggests that HAMC limits the contact required to the host retina for transfer.					
37163876	4	54	theme	material	641:648	arg1	transfer					650:657	material transfer	641:657	material transfer	641:657	Biomaterials, such as hyaluronan-based hydrogels, can support donor cell survival but have not been evaluated for effects on material transfer.					
36639086	0	0	theme	cambium	87:93	arg1	tissues					95:101	cultured poplar cambium tissues	71:101	cultured poplar cambium tissues	71:101	Investigation of chemical linkages between lignin and carbohydrates in cultured poplar cambium tissues via double isotope labeling.					
36639086	10	1	theme	bonds	1423:1427	arg1	existence					1374:1382	the existence	1370:1382	the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose	1370:1456	Combined with the carbohydrate composition and XRD analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose.					
36639086	10	2	theme	GL	1341:1342	arg1	components					1344:1353	the GL components	1337:1353	the GL components	1337:1353	Combined with the carbohydrate composition and XRD analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose.					
36639086	5	3	theme	labeled	504:510	arg1	tissue					522:527	The labeled new xylem tissue	500:527	The labeled new xylem tissue	500:527	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	10	4	theme	bonds	1394:1398	arg1	existence					1374:1382	the existence	1370:1382	the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose	1370:1456	Combined with the carbohydrate composition and XRD analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose.					
36639086	6	5	theme	ionic	817:821	arg1	separation					830:839	the ionic liquid separation	813:839	the ionic liquid separation	813:839	The X-ray diffraction (XRD) results indicated that the crystalline form of cellulose in the GL component was transformed from type I to type II after the ionic liquid separation.					
36639086	0	6	theme	poplar	80:85	arg1	tissues					95:101	cultured poplar cambium tissues	71:101	cultured poplar cambium tissues	71:101	Investigation of chemical linkages between lignin and carbohydrates in cultured poplar cambium tissues via double isotope labeling.					
36639086	4	7	theme	new	489:491	arg1	xylem					493:497	new xylem	489:497	new xylem	489:497	The results showed that the labeled precursors could be normally involved in the formation of new xylem.					
36639086	5	8	theme	DMSO/TBAH	562:570	arg1	system					572:577	ionic liquid DMSO/TBAH system	549:577	ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL)	549:660	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	6	9	theme	liquid	823:828	arg1	separation					830:839	the ionic liquid separation	813:839	the ionic liquid separation	813:839	The X-ray diffraction (XRD) results indicated that the crystalline form of cellulose in the GL component was transformed from type I to type II after the ionic liquid separation.					
36639086	6	10	theme	X-ray	667:671	arg1	XRD					686:688	XRD	686:688	XRD	686:688	The X-ray diffraction (XRD) results indicated that the crystalline form of cellulose in the GL component was transformed from type I to type II after the ionic liquid separation.					
36639086	6	10	theme	X-ray	667:671	arg1	diffraction					673:683	The X-ray diffraction	663:683	The X-ray diffraction (XRD) results	663:697	The X-ray diffraction (XRD) results indicated that the crystalline form of cellulose in the GL component was transformed from type I to type II after the ionic liquid separation.					
36639086	1	11	theme	cambium	253:259	arg1	tissue					261:266	cambium tissue	253:266	cambium tissue stripped from a growing poplar	253:297	Lignin precursor labeled with 13C (coniferin-13Cα), carbohydrate precursor labeled with D (6,6-D2-glucose) were put into cambium tissue stripped from a growing poplar.					
36639086	8	12	theme	cultured	1057:1064	arg1	tissues					1066:1072	the cultured tissues	1053:1072	the cultured tissues	1053:1072	The results showed that lignin subunits in the cultured tissues were mainly connected by β-5 and β-O-4 linkages, of which the β-O-4 substructure unit predominated.					
36639086	0	13	from	carbohydrates	54:66	arg1	tissues					95:101	cultured poplar cambium tissues	71:101	cultured poplar cambium tissues	71:101	Investigation of chemical linkages between lignin and carbohydrates in cultured poplar cambium tissues via double isotope labeling.					
36639086	0	14	theme	isotope	114:120	arg1	labeling					122:129	double isotope labeling	107:129	double isotope labeling	107:129	Investigation of chemical linkages between lignin and carbohydrates in cultured poplar cambium tissues via double isotope labeling.					
36639086	6	15	theme	cellulose	738:746	arg1	form					730:733	the crystalline form	714:733	the crystalline form of cellulose in the GL component	714:766	The X-ray diffraction (XRD) results indicated that the crystalline form of cellulose in the GL component was transformed from type I to type II after the ionic liquid separation.					
36639086	0	16	theme	double	107:112	arg1	labeling					122:129	double isotope labeling	107:129	double isotope labeling	107:129	Investigation of chemical linkages between lignin and carbohydrates in cultured poplar cambium tissues via double isotope labeling.					
36639086	0	17	from	lignin	43:48	arg1	tissues					95:101	cultured poplar cambium tissues	71:101	cultured poplar cambium tissues	71:101	Investigation of chemical linkages between lignin and carbohydrates in cultured poplar cambium tissues via double isotope labeling.					
36639086	10	18	theme	analysis	1319:1326	arg1	results					1328:1334	the carbohydrate composition and XRD analysis results	1282:1334	the carbohydrate composition and XRD analysis results	1282:1334	Combined with the carbohydrate composition and XRD analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose.					
36639086	10	19	theme	carbohydrate	1286:1297	arg1	composition					1299:1309	carbohydrate composition	1286:1309	carbohydrate composition	1286:1309	Combined with the carbohydrate composition and XRD analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose.					
36639086	10	20	theme	ether	1417:1421	arg1	bonds					1423:1427	ether bonds	1417:1427	ether bonds	1417:1427	Combined with the carbohydrate composition and XRD analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose.					
36639086	10	21	theme	XRD	1315:1317	arg1	analysis					1319:1326	XRD analysis	1315:1326	XRD analysis	1315:1326	Combined with the carbohydrate composition and XRD analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose.					
36639086	10	22	theme	bonds	1407:1411	arg1	existence					1374:1382	the existence	1370:1382	the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose	1370:1456	Combined with the carbohydrate composition and XRD analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose.					
36639086	8	23	theme	substructure	1142:1153	arg1	unit					1155:1158	the β-O-4 substructure unit	1132:1158	the β-O-4 substructure unit	1132:1158	The results showed that lignin subunits in the cultured tissues were mainly connected by β-5 and β-O-4 linkages, of which the β-O-4 substructure unit predominated.					
36639086	3	24	theme	isotopic	360:367	arg1	abundance					369:377	the isotopic abundance	356:377	the isotopic abundance	356:377	Then, the isotopic abundance was determined.					
36639086	5	25	theme	glucan-lignin	605:617	arg1	GL					628:629	GL	628:629	GL	628:629	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	5	25	theme	glucan-lignin	605:617	arg1	components					593:602	two components	589:602	two components: glucan-lignin complex (GL) and xylan-lignin complex (XL)	589:660	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	5	25	theme	glucan-lignin	605:617	arg1	complex					619:625	glucan-lignin complex	605:625	glucan-lignin complex (GL)	605:630	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	5	26	theme	ionic	549:553	arg1	system					572:577	ionic liquid DMSO/TBAH system	549:577	ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL)	549:660	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	1	27	theme	carbohydrate	184:195	arg1	13C					162:164	13C	162:164	13C (coniferin-13Cα)	162:181	Lignin precursor labeled with 13C (coniferin-13Cα), carbohydrate precursor labeled with D (6,6-D2-glucose) were put into cambium tissue stripped from a growing poplar.					
36639086	1	27	theme	carbohydrate	184:195	arg1	precursor					197:205	carbohydrate precursor	184:205	carbohydrate precursor labeled with D (6,6-D2-glucose)	184:237	Lignin precursor labeled with 13C (coniferin-13Cα), carbohydrate precursor labeled with D (6,6-D2-glucose) were put into cambium tissue stripped from a growing poplar.					
36639086	0	28	theme	linkages	26:33	arg1	Investigation					0:12	Investigation	0:12	Investigation of chemical linkages between lignin and carbohydrates in cultured poplar cambium tissues via double isotope labeling.	0:130	Investigation of chemical linkages between lignin and carbohydrates in cultured poplar cambium tissues via double isotope labeling.					
36639086	6	29	theme	crystalline	718:728	arg1	form					730:733	the crystalline form	714:733	the crystalline form of cellulose in the GL component	714:766	The X-ray diffraction (XRD) results indicated that the crystalline form of cellulose in the GL component was transformed from type I to type II after the ionic liquid separation.					
36639086	7	30	theme	resonance	980:988	arg1	spectroscopy					996:1007	nuclear magnetic resonance (NMR) spectroscopy	963:1007	nuclear magnetic resonance (NMR) spectroscopy	963:1007	Then the GL and XL were purified and modified by enzymatic and chemical methods, and their structures were elucidated by nuclear magnetic resonance (NMR) spectroscopy.					
36639086	5	31	theme	liquid	555:560	arg1	system					572:577	ionic liquid DMSO/TBAH system	549:577	ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL)	549:660	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	6	32	theme	GL	755:756	arg1	component					758:766	the GL component	751:766	the GL component	751:766	The X-ray diffraction (XRD) results indicated that the crystalline form of cellulose in the GL component was transformed from type I to type II after the ionic liquid separation.					
36639086	10	33	theme	composition	1299:1309	arg1	results					1328:1334	the carbohydrate composition and XRD analysis results	1282:1334	the carbohydrate composition and XRD analysis results	1282:1334	Combined with the carbohydrate composition and XRD analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose.					
36639086	0	34	theme	chemical	17:24	arg1	linkages					26:33	chemical linkages	17:33	chemical linkages between lignin and carbohydrates in cultured poplar cambium tissues via double isotope labeling	17:129	Investigation of chemical linkages between lignin and carbohydrates in cultured poplar cambium tissues via double isotope labeling.					
36639086	10	35	theme	acetal	1387:1392	arg1	bonds					1394:1398	acetal bonds	1387:1398	acetal bonds	1387:1398	Combined with the carbohydrate composition and XRD analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose.					
36639086	4	36	theme	labeled	423:429	arg1	precursors					431:440	the labeled precursors	419:440	the labeled precursors	419:440	The results showed that the labeled precursors could be normally involved in the formation of new xylem.					
36639086	1	37	theme	growing	284:290	arg1	poplar					292:297	a growing poplar	282:297	a growing poplar	282:297	Lignin precursor labeled with 13C (coniferin-13Cα), carbohydrate precursor labeled with D (6,6-D2-glucose) were put into cambium tissue stripped from a growing poplar.					
36639086	7	38	theme	nuclear	963:969	arg1	NMR					991:993	NMR	991:993	NMR	991:993	Then the GL and XL were purified and modified by enzymatic and chemical methods, and their structures were elucidated by nuclear magnetic resonance (NMR) spectroscopy.					
36639086	7	38	theme	nuclear	963:969	arg1	resonance					980:988	nuclear magnetic resonance	963:988	nuclear magnetic resonance (NMR) spectroscopy	963:1007	Then the GL and XL were purified and modified by enzymatic and chemical methods, and their structures were elucidated by nuclear magnetic resonance (NMR) spectroscopy.					
36639086	8	39	theme	β-O-4	1136:1140	arg1	unit					1155:1158	the β-O-4 substructure unit	1132:1158	the β-O-4 substructure unit	1132:1158	The results showed that lignin subunits in the cultured tissues were mainly connected by β-5 and β-O-4 linkages, of which the β-O-4 substructure unit predominated.					
36639086	8	40	theme	β-O-4	1107:1111	arg1	linkages					1113:1120	β-O-4 linkages	1107:1120	β-O-4 linkages	1107:1120	The results showed that lignin subunits in the cultured tissues were mainly connected by β-5 and β-O-4 linkages, of which the β-O-4 substructure unit predominated.					
36639086	7	41	theme	magnetic	971:978	arg1	NMR					991:993	NMR	991:993	NMR	991:993	Then the GL and XL were purified and modified by enzymatic and chemical methods, and their structures were elucidated by nuclear magnetic resonance (NMR) spectroscopy.					
36639086	7	41	theme	magnetic	971:978	arg1	resonance					980:988	nuclear magnetic resonance	963:988	nuclear magnetic resonance (NMR) spectroscopy	963:1007	Then the GL and XL were purified and modified by enzymatic and chemical methods, and their structures were elucidated by nuclear magnetic resonance (NMR) spectroscopy.					
36639086	8	42	from	subunits	1041:1048	arg1	tissues					1066:1072	the cultured tissues	1053:1072	the cultured tissues	1053:1072	The results showed that lignin subunits in the cultured tissues were mainly connected by β-5 and β-O-4 linkages, of which the β-O-4 substructure unit predominated.					
36639086	5	43	theme	new	512:514	arg1	tissue					522:527	The labeled new xylem tissue	500:527	The labeled new xylem tissue	500:527	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	6	44	from	form	730:733	arg1	component					758:766	the GL component	751:766	the GL component	751:766	The X-ray diffraction (XRD) results indicated that the crystalline form of cellulose in the GL component was transformed from type I to type II after the ionic liquid separation.					
36639086	4	45	theme	xylem	493:497	arg1	formation					476:484	the formation	472:484	the formation of new xylem	472:497	The results showed that the labeled precursors could be normally involved in the formation of new xylem.					
36639086	9	46	theme	ether	1238:1242	arg1	bonds					1244:1248	ether bonds	1238:1248	ether bonds	1238:1248	Lignin and carbohydrates were mainly connected by acetal bonds, ether bonds, and ester bonds.					
36639086	5	47	theme	xylem	516:520	arg1	tissue					522:527	The labeled new xylem tissue	500:527	The labeled new xylem tissue	500:527	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	5	48	dep	components	593:602	arg1	complex					649:655	xylan-lignin complex	636:655	xylan-lignin complex (XL)	636:660	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	5	48	dep	components	593:602	arg1	GL					628:629	GL	628:629	GL	628:629	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	5	48	dep	components	593:602	arg1	XL					658:659	XL	658:659	XL	658:659	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	5	48	dep	components	593:602	arg1	complex					619:625	glucan-lignin complex	605:625	glucan-lignin complex (GL)	605:630	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	5	48	dep	components	593:602	arg1	components					593:602	two components	589:602	two components: glucan-lignin complex (GL) and xylan-lignin complex (XL)	589:660	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	7	49	theme	enzymatic	891:899	arg1	methods					914:920	enzymatic and chemical methods	891:920	enzymatic and chemical methods	891:920	Then the GL and XL were purified and modified by enzymatic and chemical methods, and their structures were elucidated by nuclear magnetic resonance (NMR) spectroscopy.					
36639086	5	50	theme	xylan-lignin	636:647	arg1	complex					649:655	xylan-lignin complex	636:655	xylan-lignin complex (XL)	636:660	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	5	50	theme	xylan-lignin	636:647	arg1	components					593:602	two components	589:602	two components: glucan-lignin complex (GL) and xylan-lignin complex (XL)	589:660	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	5	50	theme	xylan-lignin	636:647	arg1	XL					658:659	XL	658:659	XL	658:659	The labeled new xylem tissue was fractionated by ionic liquid DMSO/TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL).					
36639086	7	51	dep	GL	851:852	arg1	the					847:849	the	847:849	the	847:849	Then the GL and XL were purified and modified by enzymatic and chemical methods, and their structures were elucidated by nuclear magnetic resonance (NMR) spectroscopy.					
36639086	9	52	theme	acetal	1224:1229	arg1	bonds					1231:1235	acetal bonds	1224:1235	acetal bonds	1224:1235	Lignin and carbohydrates were mainly connected by acetal bonds, ether bonds, and ester bonds.					
36639086	10	53	theme	ester	1401:1405	arg1	bonds					1407:1411	ester bonds	1401:1411	ester bonds	1401:1411	Combined with the carbohydrate composition and XRD analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose.					
36639086	1	54	theme	Lignin	132:137	arg1	precursor					139:147	Lignin precursor	132:147	Lignin precursor labeled with 13C (coniferin-13Cα), carbohydrate precursor labeled with D (6,6-D2-glucose)	132:237	Lignin precursor labeled with 13C (coniferin-13Cα), carbohydrate precursor labeled with D (6,6-D2-glucose) were put into cambium tissue stripped from a growing poplar.					
36639086	10	55	dep	composition	1299:1309	arg1	the					1282:1284	the	1282:1284	the	1282:1284	Combined with the carbohydrate composition and XRD analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds between lignin and cellulose.					
36639086	0	56	theme	cultured	71:78	arg1	tissues					95:101	cultured poplar cambium tissues	71:101	cultured poplar cambium tissues	71:101	Investigation of chemical linkages between lignin and carbohydrates in cultured poplar cambium tissues via double isotope labeling.					
36639086	7	57	theme	chemical	905:912	arg1	methods					914:920	enzymatic and chemical methods	891:920	enzymatic and chemical methods	891:920	Then the GL and XL were purified and modified by enzymatic and chemical methods, and their structures were elucidated by nuclear magnetic resonance (NMR) spectroscopy.					
36639086	8	58	theme	lignin	1034:1039	arg1	subunits					1041:1048	lignin subunits	1034:1048	lignin subunits in the cultured tissues	1034:1072	The results showed that lignin subunits in the cultured tissues were mainly connected by β-5 and β-O-4 linkages, of which the β-O-4 substructure unit predominated.					
36639086	6	59	theme	diffraction	673:683	arg1	results					691:697	The X-ray diffraction (XRD) results	663:697	The X-ray diffraction (XRD) results	663:697	The X-ray diffraction (XRD) results indicated that the crystalline form of cellulose in the GL component was transformed from type I to type II after the ionic liquid separation.					
36639086	9	60	theme	ester	1255:1259	arg1	bonds					1261:1265	ester bonds	1255:1265	ester bonds	1255:1265	Lignin and carbohydrates were mainly connected by acetal bonds, ether bonds, and ester bonds.					
35714619	5	0	theme	H/H-treated	819:829	arg1	ddECM					858:862	this H/H-treated decellularized dermal ECM (ddECM)	814:863	this H/H-treated decellularized dermal ECM (ddECM)	814:863	Furthermore, this H/H-treated decellularized dermal ECM (ddECM) exhibits a residual DNA content of <50 ng mg-1 of dry tissue.					
35714619	1	1	theme	extracellular	168:180	arg1	ECMs					192:195	ECMs	192:195	ECMs	192:195	Decellularized extracellular matrices (ECMs) are being extensively used for tissue engineering purposes and detergents are predominantly used for this.					
35714619	1	1	theme	extracellular	168:180	arg1	matrices					182:189	Decellularized extracellular matrices	153:189	Decellularized extracellular matrices (ECMs)	153:196	Decellularized extracellular matrices (ECMs) are being extensively used for tissue engineering purposes and detergents are predominantly used for this.					
35714619	5	2	theme	decellularized	831:844	arg1	ddECM					858:862	this H/H-treated decellularized dermal ECM (ddECM)	814:863	this H/H-treated decellularized dermal ECM (ddECM)	814:863	Furthermore, this H/H-treated decellularized dermal ECM (ddECM) exhibits a residual DNA content of <50 ng mg-1 of dry tissue.					
35714619	6	3	theme	glycosaminoglycans	954:971	arg1	glycosaminoglycans					954:971	glycosaminoglycans	954:971	glycosaminoglycans	954:971	Moreover, 85.64 ± 3.01% of glycosaminoglycans and 65.53 ± 2.9% collagen are retained compared to the native tissue, which is higher than the ddECMs prepared by other methods.					
35714619	6	3	theme	glycosaminoglycans	954:971	arg1	%					949:949	85.64 ± 3.01%	937:949	85.64 ± 3.01% of glycosaminoglycans	937:971	Moreover, 85.64 ± 3.01% of glycosaminoglycans and 65.53 ± 2.9% collagen are retained compared to the native tissue, which is higher than the ddECMs prepared by other methods.					
35714619	6	3	theme	glycosaminoglycans	954:971	arg1	collagen					990:997	65.53 ± 2.9% collagen	977:997	65.53 ± 2.9% collagen	977:997	Moreover, 85.64 ± 3.01% of glycosaminoglycans and 65.53 ± 2.9% collagen are retained compared to the native tissue, which is higher than the ddECMs prepared by other methods.					
35714619	10	4	contain	have	1501:1504	arg2	alignment					1588:1596	excellent isotropic cellular alignment	1559:1596	excellent isotropic cellular alignment	1559:1596	The printed constructs with optimized parameters have adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment.					
35714619	10	4	contain	have	1501:1504	arg2	properties					1544:1553	adequate mechanical and cell adhesive properties	1506:1553	adequate mechanical and cell adhesive properties	1506:1553	The printed constructs with optimized parameters have adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment.					
35714619	10	4	contain	have	1501:1504	arg1	constructs					1464:1473	The printed constructs	1452:1473	The printed constructs with optimized parameters	1452:1499	The printed constructs with optimized parameters have adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment.					
35714619	7	5	theme	detergent-based	1181:1195	arg1	methods					1197:1203	detergent-based methods	1181:1203	detergent-based methods	1181:1203	The cellular response is superior in ddECM (H/H) than other ddECMs prepared by detergent-based methods.					
35714619	3	6	theme	goat	607:610	arg1	skin					612:615	goat skin	607:615	goat skin that resulted in much higher yield than other methods	607:669	An optimized, single-step, cost-effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions-based method is employed to decellularize goat skin that resulted in much higher yield than other methods.					
35714619	0	7	theme	Process	84:90	arg1	Optimization					92:103	Process Optimization	84:103	Process Optimization	84:103	Formulation of Dermal Tissue Matrix Bioink by a Facile Decellularization Method and Process Optimization for 3D Bioprinting toward Translation Research.					
35714619	8	8	theme	recovery	1303:1310	arg1	properties					1312:1321	shear recovery properties	1297:1321	shear recovery properties	1297:1321	Additionally, a bioink is formulated with the ddECM (H/H), showing good shear thinning and shear recovery properties.					
35714619	0	9	theme	3D	109:110	arg1	Bioprinting					112:122	3D Bioprinting	109:122	3D Bioprinting toward Translation Research	109:150	Formulation of Dermal Tissue Matrix Bioink by a Facile Decellularization Method and Process Optimization for 3D Bioprinting toward Translation Research.					
35714619	8	10	theme	shear	1297:1301	arg1	properties					1312:1321	shear recovery properties	1297:1321	shear recovery properties	1297:1321	Additionally, a bioink is formulated with the ddECM (H/H), showing good shear thinning and shear recovery properties.					
35714619	9	11	theme	ddECM	1438:1442	arg1	bioink					1444:1449	ddECM bioink	1438:1449	ddECM bioink	1438:1449	Process optimization in terms of print speed, flow rate, and viscosity is done to obtain a bioprinting window for ddECM bioink.					
35714619	2	12	theme	used	415:418	arg1	methods					454:460	the most used detergent-based decellularization methods	406:460	the most used detergent-based decellularization methods	406:460	A facile detergent-free decellularization method is developed for dermal matrix and compared it with the most used detergent-based decellularization methods.					
35714619	9	13	theme	bioprinting	1415:1425	arg1	window					1427:1432	a bioprinting window	1413:1432	a bioprinting window for ddECM bioink	1413:1449	Process optimization in terms of print speed, flow rate, and viscosity is done to obtain a bioprinting window for ddECM bioink.					
35714619	5	14	theme	dermal	846:851	arg1	ddECM					858:862	this H/H-treated decellularized dermal ECM (ddECM)	814:863	this H/H-treated decellularized dermal ECM (ddECM)	814:863	Furthermore, this H/H-treated decellularized dermal ECM (ddECM) exhibits a residual DNA content of <50 ng mg-1 of dry tissue.					
35714619	9	15	theme	viscosity	1385:1393	arg1	terms					1348:1352	terms	1348:1352	terms of print speed, flow rate, and viscosity	1348:1393	Process optimization in terms of print speed, flow rate, and viscosity is done to obtain a bioprinting window for ddECM bioink.					
35714619	5	16	theme	dry	915:917	arg1	tissue					919:924	dry tissue	915:924	dry tissue	915:924	Furthermore, this H/H-treated decellularized dermal ECM (ddECM) exhibits a residual DNA content of <50 ng mg-1 of dry tissue.					
35714619	9	17	from	optimization	1332:1343	arg1	terms					1348:1352	terms	1348:1352	terms of print speed, flow rate, and viscosity	1348:1393	Process optimization in terms of print speed, flow rate, and viscosity is done to obtain a bioprinting window for ddECM bioink.					
35714619	9	18	theme	speed	1363:1367	arg1	terms					1348:1352	terms	1348:1352	terms of print speed, flow rate, and viscosity	1348:1393	Process optimization in terms of print speed, flow rate, and viscosity is done to obtain a bioprinting window for ddECM bioink.					
35714619	10	19	theme	excellent	1559:1567	arg1	alignment					1588:1596	excellent isotropic cellular alignment	1559:1596	excellent isotropic cellular alignment	1559:1596	The printed constructs with optimized parameters have adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment.					
35714619	9	20	theme	Process	1324:1330	arg1	optimization					1332:1343	Process optimization	1324:1343	Process optimization in terms of print speed, flow rate, and viscosity	1324:1393	Process optimization in terms of print speed, flow rate, and viscosity is done to obtain a bioprinting window for ddECM bioink.					
35714619	5	21	theme	tissue	919:924	arg1	<50 ng mg-1					900:910	<50 ng mg-1	900:910	<50 ng mg-1 of dry tissue	900:924	Furthermore, this H/H-treated decellularized dermal ECM (ddECM) exhibits a residual DNA content of <50 ng mg-1 of dry tissue.					
35714619	6	22	theme	native	1028:1033	arg1	tissue					1035:1040	the native tissue	1024:1040	the native tissue	1024:1040	Moreover, 85.64 ± 3.01% of glycosaminoglycans and 65.53 ± 2.9% collagen are retained compared to the native tissue, which is higher than the ddECMs prepared by other methods.					
35714619	6	22	theme	native	1028:1033	arg1	higher					1052:1057	higher	1052:1057	higher	1052:1057	Moreover, 85.64 ± 3.01% of glycosaminoglycans and 65.53 ± 2.9% collagen are retained compared to the native tissue, which is higher than the ddECMs prepared by other methods.					
35714619	4	23	theme	mechanical	693:702	arg1	property					704:711	mechanical property	693:711	mechanical property	693:711	The ECM composition, mechanical property, and cytocompatibility are evaluated and compared with other decellularization methods.					
35714619	2	24	theme	decellularization	329:345	arg1	method					347:352	A facile detergent-free decellularization method	305:352	A facile detergent-free decellularization method	305:352	A facile detergent-free decellularization method is developed for dermal matrix and compared it with the most used detergent-based decellularization methods.					
35714619	3	25	theme	optimized	466:474	arg1	method					571:576	An optimized, single-step, cost-effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions-based method	463:576	An optimized, single-step, cost-effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions-based method	463:576	An optimized, single-step, cost-effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions-based method is employed to decellularize goat skin that resulted in much higher yield than other methods.					
35714619	0	26	theme	Tissue	22:27	arg1	Bioink					36:41	Dermal Tissue Matrix Bioink	15:41	Dermal Tissue Matrix Bioink	15:41	Formulation of Dermal Tissue Matrix Bioink by a Facile Decellularization Method and Process Optimization for 3D Bioprinting toward Translation Research.					
35714619	4	27	theme	decellularization	774:790	arg1	methods					792:798	other decellularization methods	768:798	other decellularization methods	768:798	The ECM composition, mechanical property, and cytocompatibility are evaluated and compared with other decellularization methods.					
35714619	7	28	theme	cellular	1106:1113	arg1	superior					1127:1134	superior	1127:1134	superior	1127:1134	The cellular response is superior in ddECM (H/H) than other ddECMs prepared by detergent-based methods.					
35714619	7	28	theme	cellular	1106:1113	arg1	response					1115:1122	The cellular response	1102:1122	The cellular response	1102:1122	The cellular response is superior in ddECM (H/H) than other ddECMs prepared by detergent-based methods.					
35714619	2	29	theme	detergent-free	314:327	arg1	method					347:352	A facile detergent-free decellularization method	305:352	A facile detergent-free decellularization method	305:352	A facile detergent-free decellularization method is developed for dermal matrix and compared it with the most used detergent-based decellularization methods.					
35714619	5	30	theme	DNA	885:887	arg1	content					889:895	a residual DNA content	874:895	a residual DNA content of <50 ng mg-1 of dry tissue	874:924	Furthermore, this H/H-treated decellularized dermal ECM (ddECM) exhibits a residual DNA content of <50 ng mg-1 of dry tissue.					
35714619	0	31	theme	Dermal	15:20	arg1	Bioink					36:41	Dermal Tissue Matrix Bioink	15:41	Dermal Tissue Matrix Bioink	15:41	Formulation of Dermal Tissue Matrix Bioink by a Facile Decellularization Method and Process Optimization for 3D Bioprinting toward Translation Research.					
35714619	9	32	theme	print	1357:1361	arg1	speed					1363:1367	print speed	1357:1367	print speed	1357:1367	Process optimization in terms of print speed, flow rate, and viscosity is done to obtain a bioprinting window for ddECM bioink.					
35714619	1	33	used	used	290:293	arg2	detergents					261:270	detergents	261:270	detergents	261:270	Decellularized extracellular matrices (ECMs) are being extensively used for tissue engineering purposes and detergents are predominantly used for this.					
35714619	2	34	theme	facile	307:312	arg1	method					347:352	A facile detergent-free decellularization method	305:352	A facile detergent-free decellularization method	305:352	A facile detergent-free decellularization method is developed for dermal matrix and compared it with the most used detergent-based decellularization methods.					
35714619	10	35	with	constructs	1464:1473	arg1	parameters					1490:1499	optimized parameters	1480:1499	optimized parameters	1480:1499	The printed constructs with optimized parameters have adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment.					
35714619	6	36	theme	other	1087:1091	arg1	methods					1093:1099	other methods	1087:1099	other methods	1087:1099	Moreover, 85.64 ± 3.01% of glycosaminoglycans and 65.53 ± 2.9% collagen are retained compared to the native tissue, which is higher than the ddECMs prepared by other methods.					
35714619	0	37	theme	Bioink	36:41	arg1	Formulation					0:10	Formulation	0:10	Formulation of Dermal Tissue Matrix Bioink by a Facile Decellularization Method and Process Optimization for 3D Bioprinting toward Translation Research.	0:151	Formulation of Dermal Tissue Matrix Bioink by a Facile Decellularization Method and Process Optimization for 3D Bioprinting toward Translation Research.					
35714619	7	38	from	ddECM	1139:1143	arg1	superior					1127:1134	superior	1127:1134	superior	1127:1134	The cellular response is superior in ddECM (H/H) than other ddECMs prepared by detergent-based methods.					
35714619	7	38	from	ddECM	1139:1143	arg1	response					1115:1122	The cellular response	1102:1122	The cellular response	1102:1122	The cellular response is superior in ddECM (H/H) than other ddECMs prepared by detergent-based methods.					
35714619	10	39	theme	optimized	1480:1488	arg1	parameters					1490:1499	optimized parameters	1480:1499	optimized parameters	1480:1499	The printed constructs with optimized parameters have adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment.					
35714619	3	40	dep	optimized	466:474	arg1	cost-effective					490:503	cost-effective	490:503	cost-effective	490:503	An optimized, single-step, cost-effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions-based method is employed to decellularize goat skin that resulted in much higher yield than other methods.					
35714619	3	40	dep	optimized	466:474	arg1	single-step					477:487	single-step	477:487	single-step	477:487	An optimized, single-step, cost-effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions-based method is employed to decellularize goat skin that resulted in much higher yield than other methods.					
35714619	2	41	theme	decellularization	436:452	arg1	methods					454:460	the most used detergent-based decellularization methods	406:460	the most used detergent-based decellularization methods	406:460	A facile detergent-free decellularization method is developed for dermal matrix and compared it with the most used detergent-based decellularization methods.					
35714619	0	42	theme	Matrix	29:34	arg1	Bioink					36:41	Dermal Tissue Matrix Bioink	15:41	Dermal Tissue Matrix Bioink	15:41	Formulation of Dermal Tissue Matrix Bioink by a Facile Decellularization Method and Process Optimization for 3D Bioprinting toward Translation Research.					
35714619	0	43	theme	Translation	131:141	arg1	Research					143:150	Translation Research	131:150	Translation Research	131:150	Formulation of Dermal Tissue Matrix Bioink by a Facile Decellularization Method and Process Optimization for 3D Bioprinting toward Translation Research.					
35714619	1	44	used	used	220:223	arg2	matrices					182:189	Decellularized extracellular matrices	153:189	Decellularized extracellular matrices (ECMs)	153:196	Decellularized extracellular matrices (ECMs) are being extensively used for tissue engineering purposes and detergents are predominantly used for this.					
35714619	1	44	used	used	220:223	arg2	ECMs					192:195	ECMs	192:195	ECMs	192:195	Decellularized extracellular matrices (ECMs) are being extensively used for tissue engineering purposes and detergents are predominantly used for this.					
35714619	5	45	theme	<50 ng mg-1	900:910	arg1	content					889:895	a residual DNA content	874:895	a residual DNA content of <50 ng mg-1 of dry tissue	874:924	Furthermore, this H/H-treated decellularized dermal ECM (ddECM) exhibits a residual DNA content of <50 ng mg-1 of dry tissue.					
35714619	2	46	theme	detergent-based	420:434	arg1	methods					454:460	the most used detergent-based decellularization methods	406:460	the most used detergent-based decellularization methods	406:460	A facile detergent-free decellularization method is developed for dermal matrix and compared it with the most used detergent-based decellularization methods.					
35714619	7	47	theme	other	1156:1160	arg1	ddECMs					1162:1167	other ddECMs	1156:1167	other ddECMs prepared by detergent-based methods	1156:1203	The cellular response is superior in ddECM (H/H) than other ddECMs prepared by detergent-based methods.					
35714619	9	48	theme	flow	1370:1373	arg1	rate					1375:1378	flow rate	1370:1378	flow rate	1370:1378	Process optimization in terms of print speed, flow rate, and viscosity is done to obtain a bioprinting window for ddECM bioink.					
35714619	7	49	from	superior	1127:1134	arg1	H/H					1146:1148	H/H	1146:1148	H/H	1146:1148	The cellular response is superior in ddECM (H/H) than other ddECMs prepared by detergent-based methods.					
35714619	7	49	from	superior	1127:1134	arg1	ddECM					1139:1143	ddECM	1139:1143	ddECM (H/H)	1139:1149	The cellular response is superior in ddECM (H/H) than other ddECMs prepared by detergent-based methods.					
35714619	2	50	theme	dermal	371:376	arg1	matrix					378:383	dermal matrix	371:383	dermal matrix	371:383	A facile detergent-free decellularization method is developed for dermal matrix and compared it with the most used detergent-based decellularization methods.					
35714619	9	51	theme	rate	1375:1378	arg1	terms					1348:1352	terms	1348:1352	terms of print speed, flow rate, and viscosity	1348:1393	Process optimization in terms of print speed, flow rate, and viscosity is done to obtain a bioprinting window for ddECM bioink.					
35714619	10	52	theme	printed	1456:1462	arg1	constructs					1464:1473	The printed constructs	1452:1473	The printed constructs with optimized parameters	1452:1499	The printed constructs with optimized parameters have adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment.					
35714619	10	53	theme	isotropic	1569:1577	arg1	alignment					1588:1596	excellent isotropic cellular alignment	1559:1596	excellent isotropic cellular alignment	1559:1596	The printed constructs with optimized parameters have adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment.					
35714619	6	54	theme	%	988:988	arg1	collagen					990:997	65.53 ± 2.9% collagen	977:997	65.53 ± 2.9% collagen	977:997	Moreover, 85.64 ± 3.01% of glycosaminoglycans and 65.53 ± 2.9% collagen are retained compared to the native tissue, which is higher than the ddECMs prepared by other methods.					
35714619	10	55	theme	mechanical	1515:1524	arg1	properties					1544:1553	adequate mechanical and cell adhesive properties	1506:1553	adequate mechanical and cell adhesive properties	1506:1553	The printed constructs with optimized parameters have adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment.					
35714619	3	56	theme	other	657:661	arg1	methods					663:669	other methods	657:669	other methods	657:669	An optimized, single-step, cost-effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions-based method is employed to decellularize goat skin that resulted in much higher yield than other methods.					
35714619	3	57	theme	higher	639:644	arg1	yield					646:650	much higher yield	634:650	much higher yield than other methods	634:669	An optimized, single-step, cost-effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions-based method is employed to decellularize goat skin that resulted in much higher yield than other methods.					
35714619	4	58	theme	other	768:772	arg1	methods					792:798	other decellularization methods	768:798	other decellularization methods	768:798	The ECM composition, mechanical property, and cytocompatibility are evaluated and compared with other decellularization methods.					
35714619	1	59	theme	tissue	229:234	arg1	purposes					248:255	tissue engineering purposes	229:255	tissue engineering purposes	229:255	Decellularized extracellular matrices (ECMs) are being extensively used for tissue engineering purposes and detergents are predominantly used for this.					
35714619	10	60	theme	adequate	1506:1513	arg1	properties					1544:1553	adequate mechanical and cell adhesive properties	1506:1553	adequate mechanical and cell adhesive properties	1506:1553	The printed constructs with optimized parameters have adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment.					
35714619	5	61	theme	residual	876:883	arg1	content					889:895	a residual DNA content	874:895	a residual DNA content of <50 ng mg-1 of dry tissue	874:924	Furthermore, this H/H-treated decellularized dermal ECM (ddECM) exhibits a residual DNA content of <50 ng mg-1 of dry tissue.					
35714619	3	62	theme	solutions-based	555:569	arg1	method					571:576	An optimized, single-step, cost-effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions-based method	463:576	An optimized, single-step, cost-effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions-based method	463:576	An optimized, single-step, cost-effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions-based method is employed to decellularize goat skin that resulted in much higher yield than other methods.					
35714619	1	63	theme	engineering	236:246	arg1	purposes					248:255	tissue engineering purposes	229:255	tissue engineering purposes	229:255	Decellularized extracellular matrices (ECMs) are being extensively used for tissue engineering purposes and detergents are predominantly used for this.					
35714619	0	64	theme	Decellularization	55:71	arg1	Method					73:78	a Facile Decellularization Method	46:78	a Facile Decellularization Method	46:78	Formulation of Dermal Tissue Matrix Bioink by a Facile Decellularization Method and Process Optimization for 3D Bioprinting toward Translation Research.					
35714619	4	65	theme	ECM	676:678	arg1	composition					680:690	The ECM composition	672:690	The ECM composition	672:690	The ECM composition, mechanical property, and cytocompatibility are evaluated and compared with other decellularization methods.					
35714619	8	66	theme	shear	1278:1282	arg1	thinning					1284:1291	good shear thinning	1273:1291	good shear thinning	1273:1291	Additionally, a bioink is formulated with the ddECM (H/H), showing good shear thinning and shear recovery properties.					
35714619	8	67	theme	good	1273:1276	arg1	thinning					1284:1291	good shear thinning	1273:1291	good shear thinning	1273:1291	Additionally, a bioink is formulated with the ddECM (H/H), showing good shear thinning and shear recovery properties.					
35714619	10	68	theme	cellular	1579:1586	arg1	alignment					1588:1596	excellent isotropic cellular alignment	1559:1596	excellent isotropic cellular alignment	1559:1596	The printed constructs with optimized parameters have adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment.					
35714619	10	69	theme	adhesive	1535:1542	arg1	properties					1544:1553	adequate mechanical and cell adhesive properties	1506:1553	adequate mechanical and cell adhesive properties	1506:1553	The printed constructs with optimized parameters have adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment.					
35714619	0	70	theme	Facile	48:53	arg1	Method					73:78	a Facile Decellularization Method	46:78	a Facile Decellularization Method	46:78	Formulation of Dermal Tissue Matrix Bioink by a Facile Decellularization Method and Process Optimization for 3D Bioprinting toward Translation Research.					
35714619	6	71	theme	65.53 ± 2.9	977:987	arg1	%					988:988	%	988:988	%	988:988	Moreover, 85.64 ± 3.01% of glycosaminoglycans and 65.53 ± 2.9% collagen are retained compared to the native tissue, which is higher than the ddECMs prepared by other methods.					
35714619	5	72	theme	ECM	853:855	arg1	ddECM					858:862	this H/H-treated decellularized dermal ECM (ddECM)	814:863	this H/H-treated decellularized dermal ECM (ddECM)	814:863	Furthermore, this H/H-treated decellularized dermal ECM (ddECM) exhibits a residual DNA content of <50 ng mg-1 of dry tissue.					
35714619	1	73	theme	Decellularized	153:166	arg1	ECMs					192:195	ECMs	192:195	ECMs	192:195	Decellularized extracellular matrices (ECMs) are being extensively used for tissue engineering purposes and detergents are predominantly used for this.					
35714619	1	73	theme	Decellularized	153:166	arg1	matrices					182:189	Decellularized extracellular matrices	153:189	Decellularized extracellular matrices (ECMs)	153:196	Decellularized extracellular matrices (ECMs) are being extensively used for tissue engineering purposes and detergents are predominantly used for this.					
36529324	4	0	theme	0.5 nM	1116:1121	arg1	value					1107:1111	detection value	1097:1111	detection value of 0.5 nM	1097:1121	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	3	1	theme	synthesized	666:676	arg1	nanocatalyst					678:689	the synthesized nanocatalyst	662:689	the synthesized nanocatalyst	662:689	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	5	2	theme	optimization	1139:1150	arg1	studies					1152:1158	optimization studies	1139:1158	optimization studies	1139:1158	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	2	3	theme	nanofiber	454:462	arg1	Fe3O4/CNF					475:483	Fe3O4/CNF	475:483	Fe3O4/CNF	475:483	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	2	3	theme	nanofiber	454:462	arg1	composite					464:472	cellulose nanofiber composite	444:472	cellulose nanofiber composite (Fe3O4/CNF)	444:484	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	2	3	theme	nanofiber	454:462	arg1	amplifier					492:500	an amplifier	489:500	an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection	489:581	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	4	4	used	used	977:980	arg2	sensor					950:955	The ultimate electrochemical sensor	921:955	The ultimate electrochemical sensor (Fe3O4/CNF/CPE)	921:971	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	4	4	used	used	977:980	arg2	sensor					1010:1015	a potent electrochemical sensor	985:1015	a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM	985:1121	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	4	4	used	used	977:980	arg2	Fe3O4/CNF/CPE					958:970	Fe3O4/CNF/CPE	958:970	Fe3O4/CNF/CPE	958:970	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	6	5	theme	suggested	1557:1565	arg1	capability					1567:1576	the suggested capability	1553:1576	the suggested capability of Fe3O4/CNF/CPE to detect 4-CP	1553:1608	By using the standard addition procedure on samples of drinking water and wastewater, the suggested capability of Fe3O4/CNF/CPE to detect 4-CP was further investigated.					
36529324	5	6	theme	ideal	1345:1349	arg1	conditions					1361:1370	ideal operating conditions	1345:1370	ideal operating conditions	1345:1370	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	1	7	theme	crucial	157:163	arg1	problem					165:171	A crucial problem	155:171	A crucial problem that needs to be resolved	155:197	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	1	7	theme	crucial	157:163	arg1	monitoring					230:239	the sensitive and selective monitoring	202:239	the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP),	202:302	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	5	8	theme	ideal	1198:1202	arg1	Fe3O4/CNF					1168:1176	8.0 mg Fe3O4/CNF	1161:1176	8.0 mg Fe3O4/CNF	1161:1176	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	5	8	theme	ideal	1198:1202	arg1	concentration					1213:1225	the ideal catalyst concentration	1194:1225	the ideal catalyst concentration	1194:1225	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	1	9	theme	chlorophenol	244:255	arg1	compounds					257:265	chlorophenol compounds	244:265	chlorophenol compounds	244:265	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	1	9	theme	chlorophenol	244:255	arg1	4-chlorophenol					281:294	4-chlorophenol	281:294	specifically 4-chlorophenol (4-CP)	268:301	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	1	10	theme	used	331:334	arg1	chemicals					355:363	the most frequently used organic industrial chemicals	311:363	the most frequently used organic industrial chemicals	311:363	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	5	11	theme	bare	1402:1405	arg1	CPE					1407:1409	bare CPE	1402:1409	bare CPE	1402:1409	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	5	12	theme	operating	1351:1359	arg1	conditions					1361:1370	ideal operating conditions	1345:1370	ideal operating conditions	1345:1370	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	5	13	theme	catalyst	1204:1211	arg1	Fe3O4/CNF					1168:1176	8.0 mg Fe3O4/CNF	1161:1176	8.0 mg Fe3O4/CNF	1161:1176	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	5	13	theme	catalyst	1204:1211	arg1	concentration					1213:1225	the ideal catalyst concentration	1194:1225	the ideal catalyst concentration	1194:1225	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	1	14	theme	compounds	257:265	arg1	problem					165:171	A crucial problem	155:171	A crucial problem that needs to be resolved	155:197	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	1	14	theme	compounds	257:265	arg1	monitoring					230:239	the sensitive and selective monitoring	202:239	the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP),	202:302	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	1	15	theme	organic	336:342	arg1	chemicals					355:363	the most frequently used organic industrial chemicals	311:363	the most frequently used organic industrial chemicals	311:363	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	2	16	theme	electrode	548:556	arg1	development					509:519	the development	505:519	the development of a modified carbon paste electrode (CPE) for 4-CP detection	505:581	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	5	17	theme	ideal	1263:1267	arg1	current					1294:1300	the ideal pH. The 4-CP's oxidation current	1259:1300	the ideal pH. The 4-CP's oxidation current	1259:1300	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	5	17	theme	ideal	1263:1267	arg1	times					1328:1332	times	1328:1332	times	1328:1332	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	5	18	theme	8.0 mg	1161:1166	arg1	result					1129:1134	a result	1127:1134	a result of optimization studies	1127:1158	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	5	18	theme	8.0 mg	1161:1166	arg1	concentration					1213:1225	the ideal catalyst concentration	1194:1225	the ideal catalyst concentration	1194:1225	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	5	18	theme	8.0 mg	1161:1166	arg1	Fe3O4/CNF					1168:1176	8.0 mg Fe3O4/CNF	1161:1176	8.0 mg Fe3O4/CNF	1161:1176	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	1	19	theme	industrial	344:353	arg1	chemicals					355:363	the most frequently used organic industrial chemicals	311:363	the most frequently used organic industrial chemicals	311:363	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	8	20	theme	electrochemical	1782:1796	arg1	sensors					1798:1804	electrochemical sensors	1782:1804	electrochemical sensors resulting in beneficial environmental impact and enhancing human health	1782:1876	This study paves the way for the customization of advanced nanostructure for the application in electrochemical sensors resulting in beneficial environmental impact and enhancing human health.					
36529324	4	21	theme	electrochemical	994:1008	arg1	sensor					1010:1015	a potent electrochemical sensor	985:1015	a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM	985:1121	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	4	21	theme	electrochemical	994:1008	arg1	sensor					950:955	The ultimate electrochemical sensor	921:955	The ultimate electrochemical sensor (Fe3O4/CNF/CPE)	921:971	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	5	22	theme	pH.	1269:1271	arg1	4-CP					1277:1280	pH. The 4-CP's	1269:1282	the ideal pH. The 4-CP's oxidation current	1259:1300	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	1	23	theme	chemicals	355:363	arg1	chemicals					355:363	the most frequently used organic industrial chemicals	311:363	the most frequently used organic industrial chemicals	311:363	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	1	23	theme	chemicals	355:363	arg1	one					304:306	one	304:306	one	304:306	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	6	24	theme	Fe3O4/CNF/CPE	1581:1593	arg1	capability					1567:1576	the suggested capability	1553:1576	the suggested capability of Fe3O4/CNF/CPE to detect 4-CP	1553:1608	By using the standard addition procedure on samples of drinking water and wastewater, the suggested capability of Fe3O4/CNF/CPE to detect 4-CP was further investigated.					
36529324	5	25	theme	1.67	1323:1326	arg1	current					1294:1300	the ideal pH. The 4-CP's oxidation current	1259:1300	the ideal pH. The 4-CP's oxidation current	1259:1300	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	5	25	theme	1.67	1323:1326	arg1	times					1328:1332	times	1328:1332	times	1328:1332	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	4	26	theme	detection	1097:1105	arg1	value					1107:1111	detection value	1097:1111	detection value of 0.5 nM	1097:1121	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	3	27	theme	voltammetry	717:727	arg1	techniques					817:826	differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques	698:826	differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques	698:826	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	0	28	theme	paste	77:81	arg1	electrode					83:91	carbon paste electrode	70:91	carbon paste electrode amplified with Fe3O4	70:112	Electrochemical monitoring of 4-chlorophenol as a water pollutant via carbon paste electrode amplified with Fe3O4 incorporated cellulose nanofibers (CNF).					
36529324	2	29	theme	4-CP	568:571	arg1	detection					573:581	4-CP detection	568:581	4-CP detection	568:581	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	4	30	theme	ultimate	925:932	arg1	Fe3O4/CNF/CPE					958:970	Fe3O4/CNF/CPE	958:970	Fe3O4/CNF/CPE	958:970	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	4	30	theme	ultimate	925:932	arg1	sensor					1010:1015	a potent electrochemical sensor	985:1015	a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM	985:1121	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	4	30	theme	ultimate	925:932	arg1	sensor					950:955	The ultimate electrochemical sensor	921:955	The ultimate electrochemical sensor (Fe3O4/CNF/CPE)	921:971	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	3	31	theme	nanocatalyst	678:689	arg1	morphology					648:657	the morphology	644:657	the morphology of the synthesized nanocatalyst	644:689	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	0	32	theme	Electrochemical	0:14	arg1	monitoring					16:25	Electrochemical monitoring	0:25	Electrochemical monitoring of 4-chlorophenol as a water pollutant via carbon paste electrode amplified with Fe3O4	0:112	Electrochemical monitoring of 4-chlorophenol as a water pollutant via carbon paste electrode amplified with Fe3O4 incorporated cellulose nanofibers (CNF).					
36529324	3	33	theme	differential	698:709	arg1	DPV					730:732	DPV	730:732	DPV	730:732	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	3	33	theme	differential	698:709	arg1	voltammetry					717:727	differential pulse voltammetry	698:727	differential pulse voltammetry (DPV)	698:733	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	7	34	theme	recovery	1640:1647	arg1	range					1649:1653	The recovery range	1636:1653	The recovery range	1636:1653	The recovery range was found to be 98.52-103.66%.					
36529324	7	34	theme	recovery	1640:1647	arg1	%					1683:1683	98.52-103.66%	1671:1683	98.52-103.66%	1671:1683	The recovery range was found to be 98.52-103.66%.					
36529324	8	35	theme	enhancing	1855:1863	arg1	health					1871:1876	enhancing human health	1855:1876	enhancing human health	1855:1876	This study paves the way for the customization of advanced nanostructure for the application in electrochemical sensors resulting in beneficial environmental impact and enhancing human health.					
36529324	8	36	theme	nanostructure	1745:1757	arg1	customization					1719:1731	the customization	1715:1731	the customization of advanced nanostructure	1715:1757	This study paves the way for the customization of advanced nanostructure for the application in electrochemical sensors resulting in beneficial environmental impact and enhancing human health.					
36529324	0	37	theme	4-chlorophenol	30:43	arg1	monitoring					16:25	Electrochemical monitoring	0:25	Electrochemical monitoring of 4-chlorophenol as a water pollutant via carbon paste electrode amplified with Fe3O4	0:112	Electrochemical monitoring of 4-chlorophenol as a water pollutant via carbon paste electrode amplified with Fe3O4 incorporated cellulose nanofibers (CNF).					
36529324	3	38	theme	pulse	711:715	arg1	DPV					730:732	DPV	730:732	DPV	730:732	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	3	38	theme	pulse	711:715	arg1	voltammetry					717:727	differential pulse voltammetry	698:727	differential pulse voltammetry (DPV)	698:733	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	8	39	theme	beneficial	1819:1828	arg1	impact					1844:1849	beneficial environmental impact	1819:1849	beneficial environmental impact	1819:1849	This study paves the way for the customization of advanced nanostructure for the application in electrochemical sensors resulting in beneficial environmental impact and enhancing human health.					
36529324	5	40	theme	oxidation	1284:1292	arg1	current					1294:1300	the ideal pH. The 4-CP's oxidation current	1259:1300	the ideal pH. The 4-CP's oxidation current	1259:1300	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	5	40	theme	oxidation	1284:1292	arg1	times					1328:1332	times	1328:1332	times	1328:1332	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	0	41	theme	cellulose	127:135	arg1	CNF					149:151	CNF	149:151	CNF	149:151	Electrochemical monitoring of 4-chlorophenol as a water pollutant via carbon paste electrode amplified with Fe3O4 incorporated cellulose nanofibers (CNF).					
36529324	0	41	theme	cellulose	127:135	arg1	nanofibers					137:146	cellulose nanofibers	127:146	cellulose nanofibers (CNF)	127:152	Electrochemical monitoring of 4-chlorophenol as a water pollutant via carbon paste electrode amplified with Fe3O4 incorporated cellulose nanofibers (CNF).					
36529324	4	42	theme	1.0 nM-170 μM	1067:1079	arg1	range					1058:1062	the concentration range	1040:1062	the concentration range of 1.0 nM-170 μM	1040:1079	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	4	43	theme	potent	987:992	arg1	sensor					1010:1015	a potent electrochemical sensor	985:1015	a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM	985:1121	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	4	43	theme	potent	987:992	arg1	sensor					950:955	The ultimate electrochemical sensor	921:955	The ultimate electrochemical sensor (Fe3O4/CNF/CPE)	921:971	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	2	44	theme	cellulose	444:452	arg1	Fe3O4/CNF					475:483	Fe3O4/CNF	475:483	Fe3O4/CNF	475:483	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	2	44	theme	cellulose	444:452	arg1	composite					464:472	cellulose nanofiber composite	444:472	cellulose nanofiber composite (Fe3O4/CNF)	444:484	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	2	44	theme	cellulose	444:452	arg1	amplifier					492:500	an amplifier	489:500	an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection	489:581	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	1	45	dep	monitoring	230:239	arg1	chemicals					355:363	the most frequently used organic industrial chemicals	311:363	the most frequently used organic industrial chemicals	311:363	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	1	45	dep	monitoring	230:239	arg1	one					304:306	one	304:306	one	304:306	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	3	46	theme	electrochemical	863:877	arg1	characteristics					879:893	the electrochemical characteristics	859:893	the electrochemical characteristics of the fabricated sensor	859:918	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	4	47	theme	electrochemical	934:948	arg1	Fe3O4/CNF/CPE					958:970	Fe3O4/CNF/CPE	958:970	Fe3O4/CNF/CPE	958:970	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	4	47	theme	electrochemical	934:948	arg1	sensor					1010:1015	a potent electrochemical sensor	985:1015	a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM	985:1121	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	4	47	theme	electrochemical	934:948	arg1	sensor					950:955	The ultimate electrochemical sensor	921:955	The ultimate electrochemical sensor (Fe3O4/CNF/CPE)	921:971	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	5	48	theme	studies	1152:1158	arg1	result					1129:1134	a result	1127:1134	a result of optimization studies	1127:1158	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	5	48	theme	studies	1152:1158	arg1	Fe3O4/CNF					1168:1176	8.0 mg Fe3O4/CNF	1161:1176	8.0 mg Fe3O4/CNF	1161:1176	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	3	49	theme	Transmission	584:595	arg1	microscopy					606:615	Transmission electron microscopy	584:615	Transmission electron microscopy (TEM)	584:621	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	3	49	theme	Transmission	584:595	arg1	TEM					618:620	TEM	618:620	TEM	618:620	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	2	50	theme	study	401:405	arg1	goal					388:391	the goal	384:391	the goal of this study	384:405	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	3	51	theme	sensor	913:918	arg1	characteristics					879:893	the electrochemical characteristics	859:893	the electrochemical characteristics of the fabricated sensor	859:918	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	8	52	theme	advanced	1736:1743	arg1	nanostructure					1745:1757	advanced nanostructure	1736:1757	advanced nanostructure	1736:1757	This study paves the way for the customization of advanced nanostructure for the application in electrochemical sensors resulting in beneficial environmental impact and enhancing human health.					
36529324	2	53	theme	paste	542:546	arg1	CPE					559:561	CPE	559:561	CPE	559:561	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	2	53	theme	paste	542:546	arg1	electrode					548:556	a modified carbon paste electrode	524:556	a modified carbon paste electrode (CPE) for 4-CP detection	524:581	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	3	54	theme	electron	597:604	arg1	microscopy					606:615	Transmission electron microscopy	584:615	Transmission electron microscopy (TEM)	584:621	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	3	54	theme	electron	597:604	arg1	TEM					618:620	TEM	618:620	TEM	618:620	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	6	55	theme	addition	1489:1496	arg1	procedure					1498:1506	the standard addition procedure	1476:1506	the standard addition procedure on samples of drinking water and wastewater	1476:1550	By using the standard addition procedure on samples of drinking water and wastewater, the suggested capability of Fe3O4/CNF/CPE to detect 4-CP was further investigated.					
36529324	0	56	theme	water	50:54	arg1	pollutant					56:64	a water pollutant	48:64	a water pollutant via carbon paste electrode amplified with Fe3O4	48:112	Electrochemical monitoring of 4-chlorophenol as a water pollutant via carbon paste electrode amplified with Fe3O4 incorporated cellulose nanofibers (CNF).					
36529324	3	57	used	used	627:630	arg2	TEM					618:620	TEM	618:620	TEM	618:620	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	3	57	used	used	627:630	arg2	microscopy					606:615	Transmission electron microscopy	584:615	Transmission electron microscopy (TEM)	584:621	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	2	58	theme	carbon	535:540	arg1	CPE					559:561	CPE	559:561	CPE	559:561	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	2	58	theme	carbon	535:540	arg1	electrode					548:556	a modified carbon paste electrode	524:556	a modified carbon paste electrode (CPE) for 4-CP detection	524:581	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	8	59	from	application	1767:1777	arg1	sensors					1798:1804	electrochemical sensors	1782:1804	electrochemical sensors resulting in beneficial environmental impact and enhancing human health	1782:1876	This study paves the way for the customization of advanced nanostructure for the application in electrochemical sensors resulting in beneficial environmental impact and enhancing human health.					
36529324	6	60	theme	standard	1480:1487	arg1	procedure					1498:1506	the standard addition procedure	1476:1506	the standard addition procedure on samples of drinking water and wastewater	1476:1550	By using the standard addition procedure on samples of drinking water and wastewater, the suggested capability of Fe3O4/CNF/CPE to detect 4-CP was further investigated.					
36529324	3	61	theme	sweep	793:797	arg1	LSV					812:814	LSV	812:814	LSV	812:814	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	3	61	theme	sweep	793:797	arg1	voltammetry					799:809	linear sweep voltammetry	786:809	linear sweep voltammetry (LSV)	786:815	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	5	62	theme	CPE	1407:1409	arg1	surface					1391:1397	the surface	1387:1397	the surface of bare CPE	1387:1409	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	2	63	theme	modified	526:533	arg1	CPE					559:561	CPE	559:561	CPE	559:561	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	2	63	theme	modified	526:533	arg1	electrode					548:556	a modified carbon paste electrode	524:556	a modified carbon paste electrode (CPE) for 4-CP detection	524:581	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	8	64	theme	environmental	1830:1842	arg1	impact					1844:1849	beneficial environmental impact	1819:1849	beneficial environmental impact	1819:1849	This study paves the way for the customization of advanced nanostructure for the application in electrochemical sensors resulting in beneficial environmental impact and enhancing human health.					
36529324	3	65	theme	fabricated	902:911	arg1	sensor					913:918	the fabricated sensor	898:918	the fabricated sensor	898:918	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	6	66	from	procedure	1498:1506	arg1	samples					1511:1517	samples	1511:1517	samples of drinking water and wastewater	1511:1550	By using the standard addition procedure on samples of drinking water and wastewater, the suggested capability of Fe3O4/CNF/CPE to detect 4-CP was further investigated.					
36529324	3	67	theme	voltammetry	799:809	arg1	techniques					817:826	differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques	698:826	differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques	698:826	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	1	68	theme	sensitive	206:214	arg1	problem					165:171	A crucial problem	155:171	A crucial problem that needs to be resolved	155:197	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	1	68	theme	sensitive	206:214	arg1	monitoring					230:239	the sensitive and selective monitoring	202:239	the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP),	202:302	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	5	69	theme	oxidation	1420:1428	arg1	potential					1430:1438	its oxidation potential	1416:1438	its oxidation potential	1416:1438	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	2	70	from	amplifier	492:500	arg1	development					509:519	the development	505:519	the development of a modified carbon paste electrode (CPE) for 4-CP detection	505:581	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	4	71	from	4-CP	1032:1035	arg1	range					1058:1062	the concentration range	1040:1062	the concentration range of 1.0 nM-170 μM	1040:1079	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	4	72	theme	concentration	1044:1056	arg1	range					1058:1062	the concentration range	1040:1062	the concentration range of 1.0 nM-170 μM	1040:1079	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	3	73	theme	spectroscopy	762:773	arg1	techniques					817:826	differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques	698:826	differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques	698:826	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	0	74	theme	carbon	70:75	arg1	electrode					83:91	carbon paste electrode	70:91	carbon paste electrode amplified with Fe3O4	70:112	Electrochemical monitoring of 4-chlorophenol as a water pollutant via carbon paste electrode amplified with Fe3O4 incorporated cellulose nanofibers (CNF).					
36529324	5	75	dep	times	1328:1332	arg1	greater					1334:1340	greater	1334:1340	greater	1334:1340	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	2	76	dep	this	378:381	arg1	light					369:373	light	369:373	light	369:373	In light of this, the goal of this study was to synthesize Fe3O4 incorporated cellulose nanofiber composite (Fe3O4/CNF) as an amplifier in the development of a modified carbon paste electrode (CPE) for 4-CP detection.					
36529324	4	77	theme	value	1107:1111	arg1	limit					1088:1092	a limit	1086:1092	a limit of detection value of 0.5 nM	1086:1121	The ultimate electrochemical sensor (Fe3O4/CNF/CPE) was used as a potent electrochemical sensor for monitoring 4-CP in the concentration range of 1.0 nM-170 μM with a limit of detection value of 0.5 nM.					
36529324	5	78	from	conditions	1361:1370	arg1	current					1294:1300	the ideal pH. The 4-CP's oxidation current	1259:1300	the ideal pH. The 4-CP's oxidation current	1259:1300	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	5	78	from	conditions	1361:1370	arg1	times					1328:1332	times	1328:1332	times	1328:1332	As a result of optimization studies, 8.0 mg Fe3O4/CNF was found to be the ideal catalyst concentration, whereas pH = 6.0 was chosen as the ideal pH. The 4-CP's oxidation current was found to be over 1.67 times greater at ideal operating conditions than it was at the surface of bare CPE, and its oxidation potential decreased by about 120 mV.					
36529324	3	79	theme	linear	786:791	arg1	LSV					812:814	LSV	812:814	LSV	812:814	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	3	79	theme	linear	786:791	arg1	voltammetry					799:809	linear sweep voltammetry	786:809	linear sweep voltammetry (LSV)	786:815	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	1	80	theme	selective	220:228	arg1	problem					165:171	A crucial problem	155:171	A crucial problem that needs to be resolved	155:197	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	1	80	theme	selective	220:228	arg1	monitoring					230:239	the sensitive and selective monitoring	202:239	the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP),	202:302	A crucial problem that needs to be resolved is the sensitive and selective monitoring of chlorophenol compounds, specifically 4-chlorophenol (4-CP), one of the most frequently used organic industrial chemicals.					
36529324	8	81	theme	human	1865:1869	arg1	health					1871:1876	enhancing human health	1855:1876	enhancing human health	1855:1876	This study paves the way for the customization of advanced nanostructure for the application in electrochemical sensors resulting in beneficial environmental impact and enhancing human health.					
36529324	3	82	theme	impedance	752:760	arg1	EIS					776:778	EIS	776:778	EIS	776:778	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	3	82	theme	impedance	752:760	arg1	spectroscopy					762:773	electrochemical impedance spectroscopy	736:773	electrochemical impedance spectroscopy (EIS)	736:779	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	3	83	theme	electrochemical	736:750	arg1	EIS					776:778	EIS	776:778	EIS	776:778	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36529324	3	83	theme	electrochemical	736:750	arg1	spectroscopy					762:773	electrochemical impedance spectroscopy	736:773	electrochemical impedance spectroscopy (EIS)	736:779	Transmission electron microscopy (TEM) was used to evaluate the morphology of the synthesized nanocatalyst, while differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) techniques were implemented to illuminate the electrochemical characteristics of the fabricated sensor.					
36933583	6	0	from	IL-6	1054:1057	arg1	macrophages					1079:1089	macrophages	1079:1089	macrophages	1079:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	2	1	theme	strong	399:404	arg1	responses					444:452	strong cellular, humoral, and mucosal immune responses	399:452	strong cellular, humoral, and mucosal immune responses	399:452	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	6	2	theme	NP-induced	1014:1023	arg1	related					1103:1109	related	1103:1109	related	1103:1109	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	6	2	theme	NP-induced	1014:1023	arg1	expression					1025:1034	the NP-induced expression	1010:1034	the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages	1010:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	4	3	theme	N-2-HACC/CMCS	711:723	arg1	NPs					725:727	the N-2-HACC/CMCS NPs	707:727	the N-2-HACC/CMCS NPs	707:727	We showed that the N-2-HACC/CMCS NPs could be taken up by RAW264.7 cells and produced high levels of IL-6, IL-12p40, and TNF-α.					
36933583	7	4	theme	innate	1329:1334	arg1	response					1343:1350	the innate immune response	1325:1350	the innate immune response	1325:1350	These findings provide a reference for chitosan derivative nanomaterials as vaccine adjuvants and delivery systems and demonstrate that N-2-HACC/CMCS NPs can engage the STING-cGAS pathway to trigger the innate immune response.					
36933583	6	5	from	expression	1025:1034	arg1	macrophages					1079:1089	macrophages	1079:1089	macrophages	1079:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	4	6	theme	high	778:781	arg1	levels					783:788	high levels	778:788	high levels of IL-6, IL-12p40, and TNF-α	778:817	We showed that the N-2-HACC/CMCS NPs could be taken up by RAW264.7 cells and produced high levels of IL-6, IL-12p40, and TNF-α.					
36933583	6	7	from	IL-1β	1047:1051	arg1	macrophages					1079:1089	macrophages	1079:1089	macrophages	1079:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	5	8	theme	TBK1	920:923	arg1	expression					900:909	the expression	896:909	the expression of cGAS, TBK1, IRF3, and STING	896:940	The N-2-HACC/CMCS NPs activated BMDCs, promoted Th1 responses, and enhanced the expression of cGAS, TBK1, IRF3, and STING, as further demonstrated by qRT-PCR and western blotting.					
36933583	5	9	theme	STING	936:940	arg1	expression					900:909	the expression	896:909	the expression of cGAS, TBK1, IRF3, and STING	896:940	The N-2-HACC/CMCS NPs activated BMDCs, promoted Th1 responses, and enhanced the expression of cGAS, TBK1, IRF3, and STING, as further demonstrated by qRT-PCR and western blotting.					
36933583	7	10	theme	vaccine	1202:1208	arg1	adjuvants					1210:1218	vaccine adjuvants	1202:1218	vaccine adjuvants	1202:1218	These findings provide a reference for chitosan derivative nanomaterials as vaccine adjuvants and delivery systems and demonstrate that N-2-HACC/CMCS NPs can engage the STING-cGAS pathway to trigger the innate immune response.					
36933583	2	11	theme	chloride	313:320	arg1	chitosan/N					322:331	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	276:331	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	276:331	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	2	11	theme	chloride	313:320	arg1	nanoparticles					358:370	O-carboxymethyl chitosan nanoparticles	333:370	O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs)	333:390	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	6	12	theme	IL-10	1060:1064	arg1	related					1103:1109	related	1103:1109	related	1103:1109	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	6	12	theme	IL-10	1060:1064	arg1	expression					1025:1034	the NP-induced expression	1010:1034	the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages	1010:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	0	13	theme	cGAS-STING	116:125	arg1	pathway					127:133	the cGAS-STING pathway	112:133	the cGAS-STING pathway	112:133	Chitosan derivative composite nanoparticles as adjuvants enhance the cellular immune response via activation of the cGAS-STING pathway.					
36933583	6	14	from	macrophages	1079:1089	arg1	related					1103:1109	related	1103:1109	related	1103:1109	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	6	14	from	macrophages	1079:1089	arg1	expression					1025:1034	the NP-induced expression	1010:1034	the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages	1010:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	6	15	from	I-IFNs	1039:1044	arg1	macrophages					1079:1089	macrophages	1079:1089	macrophages	1079:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	2	16	theme	ammonium	304:311	arg1	chloride					313:320	N-2-hydroxypropyl trimethyl ammonium chloride	276:320	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	276:331	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	5	17	theme	Th1	868:870	arg1	responses					872:880	Th1 responses	868:880	Th1 responses	868:880	The N-2-HACC/CMCS NPs activated BMDCs, promoted Th1 responses, and enhanced the expression of cGAS, TBK1, IRF3, and STING, as further demonstrated by qRT-PCR and western blotting.					
36933583	2	18	theme	mucosal	429:435	arg1	responses					444:452	strong cellular, humoral, and mucosal immune responses	399:452	strong cellular, humoral, and mucosal immune responses	399:452	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	2	19	theme	trimethyl	294:302	arg1	chloride					313:320	N-2-hydroxypropyl trimethyl ammonium chloride	276:320	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	276:331	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	6	20	theme	I-IFNs	1039:1044	arg1	related					1103:1109	related	1103:1109	related	1103:1109	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	6	20	theme	I-IFNs	1039:1044	arg1	expression					1025:1034	the NP-induced expression	1010:1034	the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages	1010:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	0	21	theme	derivative	9:18	arg1	nanoparticles					30:42	Chitosan derivative composite nanoparticles	0:42	Chitosan derivative composite nanoparticles as adjuvants	0:55	Chitosan derivative composite nanoparticles as adjuvants enhance the cellular immune response via activation of the cGAS-STING pathway.					
36933583	7	22	theme	chitosan	1165:1172	arg1	nanomaterials					1185:1197	chitosan derivative nanomaterials	1165:1197	chitosan derivative nanomaterials as vaccine adjuvants and delivery systems	1165:1239	These findings provide a reference for chitosan derivative nanomaterials as vaccine adjuvants and delivery systems and demonstrate that N-2-HACC/CMCS NPs can engage the STING-cGAS pathway to trigger the innate immune response.					
36933583	7	23	theme	derivative	1174:1183	arg1	nanomaterials					1185:1197	chitosan derivative nanomaterials	1165:1197	chitosan derivative nanomaterials as vaccine adjuvants and delivery systems	1165:1239	These findings provide a reference for chitosan derivative nanomaterials as vaccine adjuvants and delivery systems and demonstrate that N-2-HACC/CMCS NPs can engage the STING-cGAS pathway to trigger the innate immune response.					
36933583	4	24	theme	RAW264.7	750:757	arg1	cells					759:763	RAW264.7 cells	750:763	RAW264.7 cells	750:763	We showed that the N-2-HACC/CMCS NPs could be taken up by RAW264.7 cells and produced high levels of IL-6, IL-12p40, and TNF-α.					
36933583	1	25	theme	vaccine	184:190	arg1	adjuvants					192:200	vaccine adjuvants	184:200	vaccine adjuvants	184:200	Chitosan and its derivatives are widely used in vaccine adjuvants and delivery systems.					
36933583	0	26	theme	Chitosan	0:7	arg1	nanoparticles					30:42	Chitosan derivative composite nanoparticles	0:42	Chitosan derivative composite nanoparticles as adjuvants	0:55	Chitosan derivative composite nanoparticles as adjuvants enhance the cellular immune response via activation of the cGAS-STING pathway.					
36933583	2	27	theme	chitosan	349:356	arg1	NPs					387:389	N-2-HACC/CMCS NPs	373:389	N-2-HACC/CMCS NPs	373:389	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	2	27	theme	chitosan	349:356	arg1	chitosan/N					322:331	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	276:331	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	276:331	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	2	27	theme	chitosan	349:356	arg1	nanoparticles					358:370	O-carboxymethyl chitosan nanoparticles	333:370	O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs)	333:390	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	2	28	theme	humoral	416:422	arg1	responses					444:452	strong cellular, humoral, and mucosal immune responses	399:452	strong cellular, humoral, and mucosal immune responses	399:452	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	7	29	theme	immune	1336:1341	arg1	response					1343:1350	the innate immune response	1325:1350	the innate immune response	1325:1350	These findings provide a reference for chitosan derivative nanomaterials as vaccine adjuvants and delivery systems and demonstrate that N-2-HACC/CMCS NPs can engage the STING-cGAS pathway to trigger the innate immune response.					
36933583	2	30	theme	O-carboxymethyl	333:347	arg1	NPs					387:389	N-2-HACC/CMCS NPs	373:389	N-2-HACC/CMCS NPs	373:389	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	2	30	theme	O-carboxymethyl	333:347	arg1	chitosan/N					322:331	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	276:331	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	276:331	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	2	30	theme	O-carboxymethyl	333:347	arg1	nanoparticles					358:370	O-carboxymethyl chitosan nanoparticles	333:370	O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs)	333:390	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	4	31	theme	TNF-α	813:817	arg1	levels					783:788	high levels	778:788	high levels of IL-6, IL-12p40, and TNF-α	778:817	We showed that the N-2-HACC/CMCS NPs could be taken up by RAW264.7 cells and produced high levels of IL-6, IL-12p40, and TNF-α.					
36933583	2	32	dep	antigens	232:239	arg1	encapsulated					241:252	encapsulated	241:252	encapsulated in	241:255	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	2	32	dep	antigens	232:239	arg1	conjugated					260:269	conjugated	260:269	conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs)	260:390	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	0	33	theme	composite	20:28	arg1	nanoparticles					30:42	Chitosan derivative composite nanoparticles	0:42	Chitosan derivative composite nanoparticles as adjuvants	0:55	Chitosan derivative composite nanoparticles as adjuvants enhance the cellular immune response via activation of the cGAS-STING pathway.					
36933583	0	34	theme	pathway	127:133	arg1	activation					98:107	activation	98:107	activation of the cGAS-STING pathway	98:133	Chitosan derivative composite nanoparticles as adjuvants enhance the cellular immune response via activation of the cGAS-STING pathway.					
36933583	3	35	theme	cellular	666:673	arg1	response					682:689	the cellular immune response	662:689	the cellular immune response	662:689	Therefore, the purpose of this study was to explore the molecular mechanism of composite NPs by upregulating the cGAS-STING signalling pathway to enhance the cellular immune response.					
36933583	6	36	theme	IL-1β	1047:1051	arg1	related					1103:1109	related	1103:1109	related	1103:1109	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	6	36	theme	IL-1β	1047:1051	arg1	expression					1025:1034	the NP-induced expression	1010:1034	the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages	1010:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	1	37	theme	delivery	206:213	arg1	systems					215:221	delivery systems	206:221	delivery systems	206:221	Chitosan and its derivatives are widely used in vaccine adjuvants and delivery systems.					
36933583	7	38	theme	N-2-HACC/CMCS	1262:1274	arg1	NPs					1276:1278	N-2-HACC/CMCS NPs	1262:1278	N-2-HACC/CMCS NPs	1262:1278	These findings provide a reference for chitosan derivative nanomaterials as vaccine adjuvants and delivery systems and demonstrate that N-2-HACC/CMCS NPs can engage the STING-cGAS pathway to trigger the innate immune response.					
36933583	2	39	theme	action	476:481	arg1	mechanism					463:471	the mechanism	459:471	the mechanism of action	459:481	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	3	40	theme	immune	675:680	arg1	response					682:689	the cellular immune response	662:689	the cellular immune response	662:689	Therefore, the purpose of this study was to explore the molecular mechanism of composite NPs by upregulating the cGAS-STING signalling pathway to enhance the cellular immune response.					
36933583	4	41	theme	IL-12p40	799:806	arg1	levels					783:788	high levels	778:788	high levels of IL-6, IL-12p40, and TNF-α	778:817	We showed that the N-2-HACC/CMCS NPs could be taken up by RAW264.7 cells and produced high levels of IL-6, IL-12p40, and TNF-α.					
36933583	3	42	theme	composite	587:595	arg1	NPs					597:599	composite NPs	587:599	composite NPs	587:599	Therefore, the purpose of this study was to explore the molecular mechanism of composite NPs by upregulating the cGAS-STING signalling pathway to enhance the cellular immune response.					
36933583	7	43	theme	STING-cGAS	1295:1304	arg1	pathway					1306:1312	the STING-cGAS pathway	1291:1312	the STING-cGAS pathway	1291:1312	These findings provide a reference for chitosan derivative nanomaterials as vaccine adjuvants and delivery systems and demonstrate that N-2-HACC/CMCS NPs can engage the STING-cGAS pathway to trigger the innate immune response.					
36933583	5	44	theme	western	982:988	arg1	blotting					990:997	western blotting	982:997	western blotting	982:997	The N-2-HACC/CMCS NPs activated BMDCs, promoted Th1 responses, and enhanced the expression of cGAS, TBK1, IRF3, and STING, as further demonstrated by qRT-PCR and western blotting.					
36933583	2	45	theme	immune	437:442	arg1	responses					444:452	strong cellular, humoral, and mucosal immune responses	399:452	strong cellular, humoral, and mucosal immune responses	399:452	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	3	46	theme	NPs	597:599	arg1	mechanism					574:582	the molecular mechanism	560:582	the molecular mechanism of composite NPs	560:599	Therefore, the purpose of this study was to explore the molecular mechanism of composite NPs by upregulating the cGAS-STING signalling pathway to enhance the cellular immune response.					
36933583	6	47	from	IL-10	1060:1064	arg1	macrophages					1079:1089	macrophages	1079:1089	macrophages	1079:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	5	48	theme	IRF3	926:929	arg1	expression					900:909	the expression	896:909	the expression of cGAS, TBK1, IRF3, and STING	896:940	The N-2-HACC/CMCS NPs activated BMDCs, promoted Th1 responses, and enhanced the expression of cGAS, TBK1, IRF3, and STING, as further demonstrated by qRT-PCR and western blotting.					
36933583	2	49	theme	Vaccine	224:230	arg1	antigens					232:239	Vaccine antigens	224:239	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs)	224:390	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	6	50	theme	TNF-α	1070:1074	arg1	related					1103:1109	related	1103:1109	related	1103:1109	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	6	50	theme	TNF-α	1070:1074	arg1	expression					1025:1034	the NP-induced expression	1010:1034	the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages	1010:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	3	51	theme	signalling	632:641	arg1	pathway					643:649	the cGAS-STING signalling pathway	617:649	the cGAS-STING signalling pathway to enhance the cellular immune response	617:689	Therefore, the purpose of this study was to explore the molecular mechanism of composite NPs by upregulating the cGAS-STING signalling pathway to enhance the cellular immune response.					
36933583	2	52	theme	N-2-hydroxypropyl	276:292	arg1	chloride					313:320	N-2-hydroxypropyl trimethyl ammonium chloride	276:320	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	276:331	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	6	53	from	TNF-α	1070:1074	arg1	macrophages					1079:1089	macrophages	1079:1089	macrophages	1079:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	2	54	theme	N-2-HACC/CMCS	373:385	arg1	NPs					387:389	N-2-HACC/CMCS NPs	373:389	N-2-HACC/CMCS NPs	373:389	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	2	54	theme	N-2-HACC/CMCS	373:385	arg1	nanoparticles					358:370	O-carboxymethyl chitosan nanoparticles	333:370	O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs)	333:390	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
36933583	3	55	theme	molecular	564:572	arg1	mechanism					574:582	the molecular mechanism	560:582	the molecular mechanism of composite NPs	560:599	Therefore, the purpose of this study was to explore the molecular mechanism of composite NPs by upregulating the cGAS-STING signalling pathway to enhance the cellular immune response.					
36933583	4	56	theme	IL-6	793:796	arg1	levels					783:788	high levels	778:788	high levels of IL-6, IL-12p40, and TNF-α	778:817	We showed that the N-2-HACC/CMCS NPs could be taken up by RAW264.7 cells and produced high levels of IL-6, IL-12p40, and TNF-α.					
36933583	3	57	theme	study	539:543	arg1	purpose					523:529	the purpose	519:529	the purpose of this study	519:543	Therefore, the purpose of this study was to explore the molecular mechanism of composite NPs by upregulating the cGAS-STING signalling pathway to enhance the cellular immune response.					
36933583	5	58	theme	cGAS	914:917	arg1	expression					900:909	the expression	896:909	the expression of cGAS, TBK1, IRF3, and STING	896:940	The N-2-HACC/CMCS NPs activated BMDCs, promoted Th1 responses, and enhanced the expression of cGAS, TBK1, IRF3, and STING, as further demonstrated by qRT-PCR and western blotting.					
36933583	0	59	theme	immune	78:83	arg1	response					85:92	the cellular immune response	65:92	the cellular immune response	65:92	Chitosan derivative composite nanoparticles as adjuvants enhance the cellular immune response via activation of the cGAS-STING pathway.					
36933583	6	60	theme	IL-6	1054:1057	arg1	related					1103:1109	related	1103:1109	related	1103:1109	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	6	60	theme	IL-6	1054:1057	arg1	expression					1025:1034	the NP-induced expression	1010:1034	the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages	1010:1089	Moreover, the NP-induced expression of I-IFNs, IL-1β, IL-6, IL-10 and TNF-α in macrophages was closely related to cGAS-STING.					
36933583	3	61	theme	cGAS-STING	621:630	arg1	pathway					643:649	the cGAS-STING signalling pathway	617:649	the cGAS-STING signalling pathway to enhance the cellular immune response	617:689	Therefore, the purpose of this study was to explore the molecular mechanism of composite NPs by upregulating the cGAS-STING signalling pathway to enhance the cellular immune response.					
36933583	5	62	theme	N-2-HACC/CMCS	824:836	arg1	NPs					838:840	The N-2-HACC/CMCS NPs	820:840	The N-2-HACC/CMCS NPs	820:840	The N-2-HACC/CMCS NPs activated BMDCs, promoted Th1 responses, and enhanced the expression of cGAS, TBK1, IRF3, and STING, as further demonstrated by qRT-PCR and western blotting.					
36933583	0	63	theme	cellular	69:76	arg1	response					85:92	the cellular immune response	65:92	the cellular immune response	65:92	Chitosan derivative composite nanoparticles as adjuvants enhance the cellular immune response via activation of the cGAS-STING pathway.					
36933583	1	64	used	used	176:179	arg2	derivatives					153:163	its derivatives	149:163	its derivatives	149:163	Chitosan and its derivatives are widely used in vaccine adjuvants and delivery systems.					
36933583	1	64	used	used	176:179	arg2	Chitosan					136:143	Chitosan	136:143	Chitosan	136:143	Chitosan and its derivatives are widely used in vaccine adjuvants and delivery systems.					
36933583	7	65	theme	delivery	1224:1231	arg1	systems					1233:1239	delivery systems	1224:1239	delivery systems	1224:1239	These findings provide a reference for chitosan derivative nanomaterials as vaccine adjuvants and delivery systems and demonstrate that N-2-HACC/CMCS NPs can engage the STING-cGAS pathway to trigger the innate immune response.					
36933583	2	66	theme	cellular	406:413	arg1	responses					444:452	strong cellular, humoral, and mucosal immune responses	399:452	strong cellular, humoral, and mucosal immune responses	399:452	Vaccine antigens encapsulated in or conjugated onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) induce strong cellular, humoral, and mucosal immune responses, but the mechanism of action is not fully understood.					
35202633	1	0	from	water	321:325	arg1	removal					300:306	the removal	296:306	the removal of Pb2+ from water	296:325	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	0	1	from	growth	8:13	arg1	beads					50:54	carboxymethyl chitosan beads	27:54	carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions	27:113	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.					
35202633	4	2	theme	BCMC	778:781	arg1	ZIF-8					783:787	BCMC@ZIF-8	778:787	BCMC@ZIF-8	778:787	The maximum adsorption capacity of BCMC@ZIF-8 fitted using the Langmuir model was 566.09 mg/g.					
35202633	6	3	theme	skeleton	1200:1207	arg1	fixation					1176:1183	fixation	1176:1183	fixation of the polymer skeleton	1176:1207	This study demonstrated that the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water can be improved through fixation of the polymer skeleton.					
35202633	0	4	theme	improved	60:67	arg1	adsorption					69:78	improved adsorption	60:78	improved adsorption of lead ion from aqueous solutions	60:113	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.					
35202633	1	5	theme	BCMC	280:283	arg1	adsorbent					269:277	adsorbent	269:277	adsorbent	269:277	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	1	5	theme	BCMC	280:283	arg1	ZIF-8					285:289	BCMC@ZIF-8	280:289	BCMC@ZIF-8	280:289	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	2	6	theme	removal	503:509	arg1	efficiency					511:520	the removal efficiency	499:520	the removal efficiency of Pb2+ from water	499:539	The results revealed that the utilization of the BCMC as a framework enhanced the stability of ZIF-8, and the presence of the latter in the composite improved the removal efficiency of Pb2+ from water.					
35202633	2	7	from	presence	450:457	arg1	composite					480:488	the composite	476:488	the composite	476:488	The results revealed that the utilization of the BCMC as a framework enhanced the stability of ZIF-8, and the presence of the latter in the composite improved the removal efficiency of Pb2+ from water.					
35202633	0	8	theme	lead	83:86	arg1	ion					88:90	lead ion	83:90	lead ion	83:90	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.					
35202633	1	9	theme	@	284:284	arg1	adsorbent					269:277	adsorbent	269:277	adsorbent	269:277	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	1	9	theme	@	284:284	arg1	ZIF-8					285:289	BCMC@ZIF-8	280:289	BCMC@ZIF-8	280:289	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	4	10	theme	ZIF-8	783:787	arg1	capacity					766:773	The maximum adsorption capacity	743:773	The maximum adsorption capacity of BCMC@ZIF-8 fitted using the Langmuir model	743:819	The maximum adsorption capacity of BCMC@ZIF-8 fitted using the Langmuir model was 566.09 mg/g.					
35202633	4	10	theme	ZIF-8	783:787	arg1	566.09 mg/g					825:835	566.09 mg/g	825:835	566.09 mg/g	825:835	The maximum adsorption capacity of BCMC@ZIF-8 fitted using the Langmuir model was 566.09 mg/g.					
35202633	7	11	theme	water	1316:1320	arg1	treatment					1322:1330	water treatment	1316:1330	water treatment	1316:1330	Thus, the present study offers practical and theoretical guidance for the application of MOF materials in water treatment.					
35202633	6	12	theme	metal-organic	1036:1048	arg1	MOF					1061:1063	MOF	1061:1063	MOF	1061:1063	This study demonstrated that the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water can be improved through fixation of the polymer skeleton.					
35202633	6	12	theme	metal-organic	1036:1048	arg1	framework					1050:1058	metal-organic framework	1036:1058	metal-organic framework (MOF) materials	1036:1074	This study demonstrated that the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water can be improved through fixation of the polymer skeleton.					
35202633	1	13	theme	carboxymethyl	210:222	arg1	BCMC					240:243	BCMC	240:243	BCMC	240:243	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	1	13	theme	carboxymethyl	210:222	arg1	beads					233:237	carboxymethyl chitosan beads	210:237	carboxymethyl chitosan beads (BCMC)	210:244	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	3	14	theme	spectroscopy	572:583	arg1	analysis					585:592	X-ray photoelectron spectroscopy analysis	552:592	X-ray photoelectron spectroscopy analysis	552:592	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics revealed that the adsorption mechanism included diffusion and the sharing/transfer of electrons between BCMC@ZIF-8 and Pb2+.					
35202633	4	15	theme	@	782:782	arg1	ZIF-8					783:787	BCMC@ZIF-8	778:787	BCMC@ZIF-8	778:787	The maximum adsorption capacity of BCMC@ZIF-8 fitted using the Langmuir model was 566.09 mg/g.					
35202633	7	16	theme	materials	1303:1311	arg1	application					1284:1294	the application	1280:1294	the application of MOF materials in water treatment	1280:1330	Thus, the present study offers practical and theoretical guidance for the application of MOF materials in water treatment.					
35202633	1	17	theme	chitosan	224:231	arg1	BCMC					240:243	BCMC	240:243	BCMC	240:243	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	1	17	theme	chitosan	224:231	arg1	beads					233:237	carboxymethyl chitosan beads	210:237	carboxymethyl chitosan beads (BCMC)	210:244	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	4	18	theme	maximum	747:753	arg1	capacity					766:773	The maximum adsorption capacity	743:773	The maximum adsorption capacity of BCMC@ZIF-8 fitted using the Langmuir model	743:819	The maximum adsorption capacity of BCMC@ZIF-8 fitted using the Langmuir model was 566.09 mg/g.					
35202633	4	18	theme	maximum	747:753	arg1	566.09 mg/g					825:835	566.09 mg/g	825:835	566.09 mg/g	825:835	The maximum adsorption capacity of BCMC@ZIF-8 fitted using the Langmuir model was 566.09 mg/g.					
35202633	2	19	theme	Pb2+	525:528	arg1	efficiency					511:520	the removal efficiency	499:520	the removal efficiency of Pb2+ from water	499:539	The results revealed that the utilization of the BCMC as a framework enhanced the stability of ZIF-8, and the presence of the latter in the composite improved the removal efficiency of Pb2+ from water.					
35202633	6	20	theme	materials	1066:1074	arg1	stability					1023:1031	the stability	1019:1031	the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water	1019:1150	This study demonstrated that the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water can be improved through fixation of the polymer skeleton.					
35202633	7	21	theme	present	1220:1226	arg1	study					1228:1232	the present study	1216:1232	the present study	1216:1232	Thus, the present study offers practical and theoretical guidance for the application of MOF materials in water treatment.					
35202633	6	22	theme	metals	1136:1141	arg1	removal					1119:1125	the removal	1115:1125	the removal of heavy metals in water	1115:1150	This study demonstrated that the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water can be improved through fixation of the polymer skeleton.					
35202633	0	23	theme	ion	88:90	arg1	adsorption					69:78	improved adsorption	60:78	improved adsorption of lead ion from aqueous solutions	60:113	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.					
35202633	3	24	theme	@	726:726	arg1	ZIF-8					727:731	BCMC@ZIF-8	722:731	BCMC@ZIF-8	722:731	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics revealed that the adsorption mechanism included diffusion and the sharing/transfer of electrons between BCMC@ZIF-8 and Pb2+.					
35202633	2	25	attach	presence	450:457	arg2	latter					466:471	latter	466:471	latter	466:471	The results revealed that the utilization of the BCMC as a framework enhanced the stability of ZIF-8, and the presence of the latter in the composite improved the removal efficiency of Pb2+ from water.					
35202633	2	25	attach	presence	450:457	arg1	composite					480:488	the composite	476:488	the composite	476:488	The results revealed that the utilization of the BCMC as a framework enhanced the stability of ZIF-8, and the presence of the latter in the composite improved the removal efficiency of Pb2+ from water.					
35202633	3	26	from	kinetics	609:616	arg1	Data					542:545	Data	542:545	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics	542:616	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics revealed that the adsorption mechanism included diffusion and the sharing/transfer of electrons between BCMC@ZIF-8 and Pb2+.					
35202633	2	27	theme	BCMC	389:392	arg1	utilization					370:380	the utilization	366:380	the utilization of the BCMC as a framework	366:407	The results revealed that the utilization of the BCMC as a framework enhanced the stability of ZIF-8, and the presence of the latter in the composite improved the removal efficiency of Pb2+ from water.					
35202633	1	28	from	beads	233:237	arg1	growth					156:161	the in situ growth	144:161	the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water	144:325	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	0	29	theme	In	0:1	arg1	growth					8:13	In situ growth	0:13	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.	0:114	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.					
35202633	4	30	theme	adsorption	755:764	arg1	capacity					766:773	The maximum adsorption capacity	743:773	The maximum adsorption capacity of BCMC@ZIF-8 fitted using the Langmuir model	743:819	The maximum adsorption capacity of BCMC@ZIF-8 fitted using the Langmuir model was 566.09 mg/g.					
35202633	4	30	theme	adsorption	755:764	arg1	566.09 mg/g					825:835	566.09 mg/g	825:835	566.09 mg/g	825:835	The maximum adsorption capacity of BCMC@ZIF-8 fitted using the Langmuir model was 566.09 mg/g.					
35202633	7	31	theme	MOF	1299:1301	arg1	materials					1303:1311	MOF materials	1299:1311	MOF materials in water treatment	1299:1330	Thus, the present study offers practical and theoretical guidance for the application of MOF materials in water treatment.					
35202633	1	32	theme	in	148:149	arg1	growth					156:161	the in situ growth	144:161	the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water	144:325	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	6	33	from	metals	1136:1141	arg1	water					1146:1150	water	1146:1150	water	1146:1150	This study demonstrated that the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water can be improved through fixation of the polymer skeleton.					
35202633	3	34	theme	electrons	704:712	arg1	diffusion					666:674	diffusion	666:674	diffusion	666:674	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics revealed that the adsorption mechanism included diffusion and the sharing/transfer of electrons between BCMC@ZIF-8 and Pb2+.					
35202633	3	34	theme	electrons	704:712	arg1	sharing/transfer					684:699	the sharing/transfer	680:699	the sharing/transfer of electrons between BCMC@ZIF-8 and Pb2+	680:740	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics revealed that the adsorption mechanism included diffusion and the sharing/transfer of electrons between BCMC@ZIF-8 and Pb2+.					
35202633	6	35	theme	framework	1050:1058	arg1	materials					1066:1074	metal-organic framework (MOF) materials	1036:1074	metal-organic framework (MOF) materials	1036:1074	This study demonstrated that the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water can be improved through fixation of the polymer skeleton.					
35202633	3	36	theme	adsorption	636:645	arg1	mechanism					647:655	the adsorption mechanism	632:655	the adsorption mechanism	632:655	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics revealed that the adsorption mechanism included diffusion and the sharing/transfer of electrons between BCMC@ZIF-8 and Pb2+.					
35202633	0	37	theme	aqueous	97:103	arg1	solutions					105:113	aqueous solutions	97:113	aqueous solutions	97:113	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.					
35202633	5	38	theme	experiments	853:863	arg1	Results					838:844	Results	838:844	Results of the experiments on the regeneration of the adsorbent and its stability in water	838:927	Results of the experiments on the regeneration of the adsorbent and its stability in water further indicated that BCMC improved the stability of ZIF-8.					
35202633	5	39	from	stability	910:918	arg1	Results					838:844	Results	838:844	Results of the experiments on the regeneration of the adsorbent and its stability in water	838:927	Results of the experiments on the regeneration of the adsorbent and its stability in water further indicated that BCMC improved the stability of ZIF-8.					
35202633	5	39	from	stability	910:918	arg1	water					923:927	water	923:927	water	923:927	Results of the experiments on the regeneration of the adsorbent and its stability in water further indicated that BCMC improved the stability of ZIF-8.					
35202633	7	40	theme	practical	1241:1249	arg1	guidance					1267:1274	practical and theoretical guidance	1241:1274	practical and theoretical guidance for the application of MOF materials in water treatment	1241:1330	Thus, the present study offers practical and theoretical guidance for the application of MOF materials in water treatment.					
35202633	6	41	theme	heavy	1130:1134	arg1	metals					1136:1141	heavy metals	1130:1141	heavy metals in water	1130:1150	This study demonstrated that the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water can be improved through fixation of the polymer skeleton.					
35202633	0	42	theme	ZIF-8	18:22	arg1	growth					8:13	In situ growth	0:13	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.	0:114	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.					
35202633	3	43	from	analysis	585:592	arg1	Data					542:545	Data	542:545	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics	542:616	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics revealed that the adsorption mechanism included diffusion and the sharing/transfer of electrons between BCMC@ZIF-8 and Pb2+.					
35202633	2	44	theme	ZIF-8	435:439	arg1	stability					422:430	the stability	418:430	the stability of ZIF-8	418:439	The results revealed that the utilization of the BCMC as a framework enhanced the stability of ZIF-8, and the presence of the latter in the composite improved the removal efficiency of Pb2+ from water.					
35202633	1	45	theme	Pb2+	311:314	arg1	removal					300:306	the removal	296:306	the removal of Pb2+ from water	296:325	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	1	46	dep	in	148:149	arg1	situ					151:154	situ	151:154	situ	151:154	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	1	47	theme	zeolitic	166:173	arg1	ZIF-8					200:204	ZIF-8	200:204	ZIF-8	200:204	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	1	47	theme	zeolitic	166:173	arg1	framework-8					187:197	zeolitic imidazolate framework-8	166:197	zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC)	166:244	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	0	48	theme	chitosan	41:48	arg1	beads					50:54	carboxymethyl chitosan beads	27:54	carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions	27:113	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.					
35202633	2	49	theme	latter	466:471	arg1	presence					450:457	the presence	446:457	the presence of the latter in the composite	446:488	The results revealed that the utilization of the BCMC as a framework enhanced the stability of ZIF-8, and the presence of the latter in the composite improved the removal efficiency of Pb2+ from water.					
35202633	5	50	theme	ZIF-8	983:987	arg1	stability					970:978	the stability	966:978	the stability of ZIF-8	966:987	Results of the experiments on the regeneration of the adsorbent and its stability in water further indicated that BCMC improved the stability of ZIF-8.					
35202633	0	51	theme	carboxymethyl	27:39	arg1	beads					50:54	carboxymethyl chitosan beads	27:54	carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions	27:113	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.					
35202633	6	52	theme	polymer	1192:1198	arg1	skeleton					1200:1207	the polymer skeleton	1188:1207	the polymer skeleton	1188:1207	This study demonstrated that the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water can be improved through fixation of the polymer skeleton.					
35202633	3	53	theme	X-ray	552:556	arg1	analysis					585:592	X-ray photoelectron spectroscopy analysis	552:592	X-ray photoelectron spectroscopy analysis	552:592	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics revealed that the adsorption mechanism included diffusion and the sharing/transfer of electrons between BCMC@ZIF-8 and Pb2+.					
35202633	7	54	theme	theoretical	1255:1265	arg1	guidance					1267:1274	practical and theoretical guidance	1241:1274	practical and theoretical guidance for the application of MOF materials in water treatment	1241:1330	Thus, the present study offers practical and theoretical guidance for the application of MOF materials in water treatment.					
35202633	6	55	from	water	1146:1150	arg1	removal					1119:1125	the removal	1115:1125	the removal of heavy metals in water	1115:1150	This study demonstrated that the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water can be improved through fixation of the polymer skeleton.					
35202633	6	56	from	removal	1119:1125	arg1	water					1146:1150	water	1146:1150	water	1146:1150	This study demonstrated that the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water can be improved through fixation of the polymer skeleton.					
35202633	2	57	from	efficiency	511:520	arg1	water					535:539	water	535:539	water	535:539	The results revealed that the utilization of the BCMC as a framework enhanced the stability of ZIF-8, and the presence of the latter in the composite improved the removal efficiency of Pb2+ from water.					
35202633	4	58	theme	Langmuir	806:813	arg1	model					815:819	the Langmuir model	802:819	the Langmuir model	802:819	The maximum adsorption capacity of BCMC@ZIF-8 fitted using the Langmuir model was 566.09 mg/g.					
35202633	3	59	theme	photoelectron	558:570	arg1	analysis					585:592	X-ray photoelectron spectroscopy analysis	552:592	X-ray photoelectron spectroscopy analysis	552:592	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics revealed that the adsorption mechanism included diffusion and the sharing/transfer of electrons between BCMC@ZIF-8 and Pb2+.					
35202633	1	60	from	growth	156:161	arg1	BCMC					240:243	BCMC	240:243	BCMC	240:243	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	1	60	from	growth	156:161	arg1	beads					233:237	carboxymethyl chitosan beads	210:237	carboxymethyl chitosan beads (BCMC)	210:244	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	5	61	from	experiments	853:863	arg1	regeneration					872:883	the regeneration	868:883	the regeneration of the adsorbent	868:900	Results of the experiments on the regeneration of the adsorbent and its stability in water further indicated that BCMC improved the stability of ZIF-8.					
35202633	5	61	from	experiments	853:863	arg1	stability					910:918	its stability	906:918	its stability in water	906:927	Results of the experiments on the regeneration of the adsorbent and its stability in water further indicated that BCMC improved the stability of ZIF-8.					
35202633	1	62	theme	imidazolate	175:185	arg1	ZIF-8					200:204	ZIF-8	200:204	ZIF-8	200:204	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	1	62	theme	imidazolate	175:185	arg1	framework-8					187:197	zeolitic imidazolate framework-8	166:197	zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC)	166:244	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	1	63	from	framework-8	187:197	arg1	BCMC					240:243	BCMC	240:243	BCMC	240:243	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	1	63	from	framework-8	187:197	arg1	beads					233:237	carboxymethyl chitosan beads	210:237	carboxymethyl chitosan beads (BCMC)	210:244	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	5	64	theme	adsorbent	892:900	arg1	regeneration					872:883	the regeneration	868:883	the regeneration of the adsorbent	868:900	Results of the experiments on the regeneration of the adsorbent and its stability in water further indicated that BCMC improved the stability of ZIF-8.					
35202633	5	64	theme	adsorbent	892:900	arg1	stability					910:918	its stability	906:918	its stability in water	906:927	Results of the experiments on the regeneration of the adsorbent and its stability in water further indicated that BCMC improved the stability of ZIF-8.					
35202633	2	65	from	water	535:539	arg1	Pb2+					525:528	Pb2+	525:528	Pb2+ from water	525:539	The results revealed that the utilization of the BCMC as a framework enhanced the stability of ZIF-8, and the presence of the latter in the composite improved the removal efficiency of Pb2+ from water.					
35202633	2	65	from	water	535:539	arg1	efficiency					511:520	the removal efficiency	499:520	the removal efficiency of Pb2+ from water	499:539	The results revealed that the utilization of the BCMC as a framework enhanced the stability of ZIF-8, and the presence of the latter in the composite improved the removal efficiency of Pb2+ from water.					
35202633	5	66	from	Results	838:844	arg1	regeneration					872:883	the regeneration	868:883	the regeneration of the adsorbent	868:900	Results of the experiments on the regeneration of the adsorbent and its stability in water further indicated that BCMC improved the stability of ZIF-8.					
35202633	5	66	from	Results	838:844	arg1	stability					910:918	its stability	906:918	its stability in water	906:927	Results of the experiments on the regeneration of the adsorbent and its stability in water further indicated that BCMC improved the stability of ZIF-8.					
35202633	1	67	theme	framework-8	187:197	arg1	growth					156:161	the in situ growth	144:161	the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water	144:325	In this study, a method for the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on carboxymethyl chitosan beads (BCMC) to produce a composite adsorbent (BCMC@ZIF-8) for the removal of Pb2+ from water is proposed.					
35202633	7	68	from	treatment	1322:1330	arg1	application					1284:1294	the application	1280:1294	the application of MOF materials in water treatment	1280:1330	Thus, the present study offers practical and theoretical guidance for the application of MOF materials in water treatment.					
35202633	6	69	theme	high	1093:1096	arg1	efficiencies					1098:1109	high efficiencies	1093:1109	high efficiencies for the removal of heavy metals in water	1093:1150	This study demonstrated that the stability of metal-organic framework (MOF) materials, which exhibited high efficiencies for the removal of heavy metals in water can be improved through fixation of the polymer skeleton.					
35202633	7	70	from	materials	1303:1311	arg1	treatment					1322:1330	water treatment	1316:1330	water treatment	1316:1330	Thus, the present study offers practical and theoretical guidance for the application of MOF materials in water treatment.					
35202633	7	71	from	application	1284:1294	arg1	treatment					1322:1330	water treatment	1316:1330	water treatment	1316:1330	Thus, the present study offers practical and theoretical guidance for the application of MOF materials in water treatment.					
35202633	0	72	from	solutions	105:113	arg1	adsorption					69:78	improved adsorption	60:78	improved adsorption of lead ion from aqueous solutions	60:113	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.					
35202633	0	73	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ growth of ZIF-8 on carboxymethyl chitosan beads for improved adsorption of lead ion from aqueous solutions.					
35202633	3	74	theme	adsorption	598:607	arg1	kinetics					609:616	adsorption kinetics	598:616	adsorption kinetics	598:616	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics revealed that the adsorption mechanism included diffusion and the sharing/transfer of electrons between BCMC@ZIF-8 and Pb2+.					
35202633	3	75	theme	BCMC	722:725	arg1	ZIF-8					727:731	BCMC@ZIF-8	722:731	BCMC@ZIF-8	722:731	Data from X-ray photoelectron spectroscopy analysis and adsorption kinetics revealed that the adsorption mechanism included diffusion and the sharing/transfer of electrons between BCMC@ZIF-8 and Pb2+.					
35202633	5	76	from	regeneration	872:883	arg1	Results					838:844	Results	838:844	Results of the experiments on the regeneration of the adsorbent and its stability in water	838:927	Results of the experiments on the regeneration of the adsorbent and its stability in water further indicated that BCMC improved the stability of ZIF-8.					
35202633	5	76	from	regeneration	872:883	arg1	water					923:927	water	923:927	water	923:927	Results of the experiments on the regeneration of the adsorbent and its stability in water further indicated that BCMC improved the stability of ZIF-8.					
35569054	2	0	theme	fermentable	324:334	arg1	starch					336:341	fermentable starch	324:341	fermentable starch	324:341	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	3	1	theme	hull-less	845:853	arg1	HFB					863:865	HFB	863:865	HFB	863:865	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	1	theme	hull-less	845:853	arg1	barley					855:860	hull-less barley	845:860	hull-less barley (HFB)	845:866	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	8	2	dep	%	1609:1609	arg1	β-glucan					1611:1618	β-glucan	1611:1618	0.88% to 8.54% β-glucan	1596:1618	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	15	3	theme	InsP-P	2494:2499	arg1	hydrolysis					2501:2510	InsP-P hydrolysis	2494:2510	InsP-P hydrolysis	2494:2510	Carbohydrate fermentation, thus, results in greater total tract P release from InsP-P hydrolysis.					
35569054	2	4	theme	phosphates	567:576	arg1	presence					540:547	the presence	536:547	the presence of lower inositol phosphates (InsP)	536:583	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	4	theme	phosphates	567:576	arg1	digestibility					506:518	standardized total tract digestibility	481:518	standardized total tract digestibility (STTD) of P	481:530	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	4	theme	phosphates	567:576	arg1	STTD					521:524	STTD	521:524	STTD	521:524	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	5	5	theme	detected	1239:1246	arg1	InsP2-P					1267:1273	InsP6-P to InsP2-P	1256:1273	InsP6-P to InsP2-P	1256:1273	The InsP6-2-P was calculated as the sum of all detected InsP-P (InsP6-P to InsP2-P) in the sample.					
35569054	5	5	theme	detected	1239:1246	arg1	InsP-P					1248:1253	all detected InsP-P	1235:1253	all detected InsP-P (InsP6-P to InsP2-P)	1235:1274	The InsP6-2-P was calculated as the sum of all detected InsP-P (InsP6-P to InsP2-P) in the sample.					
35569054	13	6	dep	=	2156:2156	arg1	0.03					2158:2161	0.03	2158:2161	0.03	2158:2161	Diet β-glucan content was not correlated with STTD of P (R2 = 0.03) or ATTD of InsP6 (R2 = 0.05).					
35569054	9	7	theme	greater	1762:1768	arg1	ATTD					1786:1789	greater (P < 0.05) diet ATTD	1762:1789	greater (P < 0.05) diet ATTD	1762:1789	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	2	8	theme	lower	552:556	arg1	InsP					579:582	InsP	579:582	InsP	579:582	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	8	theme	lower	552:556	arg1	phosphates					567:576	lower inositol phosphates	552:576	lower inositol phosphates (InsP)	552:583	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	8	9	theme	0.35	1658:1661	arg1	%					1653:1653	%	1653:1653	%	1653:1653	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	11	10	theme	P	1970:1970	arg1	release					1972:1978	Total tract P release	1958:1978	Total tract P release	1958:1978	Total tract P release was greater (P < 0.001) for HFB, HFA, and LFW than MFB and LFB.					
35569054	3	11	theme	Youden	631:636	arg1	square					638:643	a 6 (period) × 5 (diet) Youden square	607:643	a 6 (period) × 5 (diet) Youden square	607:643	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	9	12	theme	P	1771:1771	arg1	<					1773:1773	P < 0.05	1771:1778	P < 0.05	1771:1778	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	8	13	dep	%	1586:1586	arg1	to					1579:1580	to	1579:1580	to	1579:1580	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	8	13	dep	%	1586:1586	arg1	starch					1588:1593	starch	1588:1593	41.4% to 50.6% starch	1573:1593	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	11	14	dep	greater	1984:1990	arg1	<					1995:1995	P < 0.001	1993:2001	P < 0.001	1993:2001	Total tract P release was greater (P < 0.001) for HFB, HFA, and LFW than MFB and LFB.					
35569054	3	15	theme	Canadian	1042:1049	arg1	wheat					1070:1074	Canadian Western Red Spring wheat	1042:1074	Canadian Western Red Spring wheat (LFW)	1042:1080	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	15	theme	Canadian	1042:1049	arg1	grains					751:756	one of five cereal grains	732:756	one of five cereal grains differing in amylose, β-glucan, and fiber content	732:806	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	15	theme	Canadian	1042:1049	arg1	%					727:727	80%	725:727	80% of one of five cereal grains differing in amylose, β-glucan, and fiber content	725:806	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	15	theme	Canadian	1042:1049	arg1	LFW					1077:1079	LFW	1077:1079	LFW	1077:1079	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	12	16	contain	had	2052:2054	arg2	ATTD					2075:2078	greater (P < 0.05) ATTD	2056:2078	greater (P < 0.05) ATTD of Ca	2056:2084	The LFW had greater (P < 0.05) ATTD of Ca than LFB.					
35569054	12	16	contain	had	2052:2054	arg1	LFW					2048:2050	The LFW	2044:2050	The LFW	2044:2050	The LFW had greater (P < 0.05) ATTD of Ca than LFB.					
35569054	4	17	theme	cereal	1109:1114	arg1	grains					1116:1121	cereal grains	1109:1121	cereal grains	1109:1121	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	12	18	theme	P	2065:2065	arg1	<					2067:2067	P < 0.05	2065:2072	P < 0.05	2065:2072	The LFW had greater (P < 0.05) ATTD of Ca than LFB.					
35569054	7	19	theme	random	1528:1533	arg1	effects					1535:1541	random effects	1528:1541	random effects	1528:1541	Data were analyzed using a mixed model with diet as fixed effect, and pig and period as random effects.					
35569054	7	19	theme	random	1528:1533	arg1	model					1473:1477	a mixed model	1465:1477	a mixed model with diet	1465:1487	Data were analyzed using a mixed model with diet as fixed effect, and pig and period as random effects.					
35569054	2	20	theme	total	494:498	arg1	STTD					521:524	STTD	521:524	STTD	521:524	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	20	theme	total	494:498	arg1	digestibility					506:518	standardized total tract digestibility	481:518	standardized total tract digestibility (STTD) of P	481:530	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	14	21	theme	InsP6-2-P	2404:2412	arg1	ATTD					2380:2383	greater ATTD	2372:2383	greater ATTD of InsP6-P and sum InsP6-2-P	2372:2412	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	9	22	theme	P	1708:1708	arg1	<					1710:1710	P < 0.05	1708:1715	P < 0.05	1708:1715	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	7	23	with	model	1473:1477	arg1	diet					1484:1487	diet	1484:1487	diet	1484:1487	Data were analyzed using a mixed model with diet as fixed effect, and pig and period as random effects.					
35569054	3	24	theme	ileal-cannulated	652:667	arg1	barrows					669:675	seven ileal-cannulated barrows	646:675	seven ileal-cannulated barrows (initial BW, 27.7 kg)	646:697	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	9	25	theme	diet	1781:1784	arg1	ATTD					1786:1789	greater (P < 0.05) diet ATTD	1762:1789	greater (P < 0.05) diet ATTD	1762:1789	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	4	26	theme	DM	1098:1099	arg1	basis					1102:1106	dry matter (DM) basis	1086:1106	dry matter (DM) basis	1086:1106	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	6	27	theme	degraded	1308:1315	arg1	InsP-P					1317:1322	degraded InsP-P	1308:1322	degraded InsP-P	1308:1322	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	4	28	dep	%	1181:1181	arg1	InsP6-P					1183:1189	InsP6-P	1183:1189	0.24% to 0.50% InsP6-P	1168:1189	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	3	29	theme	Red	1059:1061	arg1	wheat					1070:1074	Canadian Western Red Spring wheat	1042:1074	Canadian Western Red Spring wheat (LFW)	1042:1080	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	29	theme	Red	1059:1061	arg1	grains					751:756	one of five cereal grains	732:756	one of five cereal grains differing in amylose, β-glucan, and fiber content	732:806	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	29	theme	Red	1059:1061	arg1	%					727:727	80%	725:727	80% of one of five cereal grains differing in amylose, β-glucan, and fiber content	725:806	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	29	theme	Red	1059:1061	arg1	LFW					1077:1079	LFW	1077:1079	LFW	1077:1079	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	8	30	dep	%	1625:1625	arg1	to					1627:1628	to	1627:1628	to	1627:1628	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	3	31	theme	fed	704:706	arg1	diets					708:712	fed diets	704:712	fed diets	704:712	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	10	32	theme	P	1848:1848	arg1	<					1850:1850	P < 0.05	1848:1855	P < 0.05	1848:1855	The ATTD of InsP6-P was greater (P < 0.05) for HFB than LFB and the ATTD of the sum InsP6-2-P was greater (P < 0.05) for HFB and HFA than LFB.					
35569054	14	33	theme	greater	2372:2378	arg1	ATTD					2380:2383	greater ATTD	2372:2383	greater ATTD of InsP6-P and sum InsP6-2-P	2372:2412	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	15	34	from	hydrolysis	2501:2510	arg1	release					2481:2487	greater total tract P release	2459:2487	greater total tract P release from InsP-P hydrolysis	2459:2510	Carbohydrate fermentation, thus, results in greater total tract P release from InsP-P hydrolysis.					
35569054	10	35	dep	greater	1913:1919	arg1	<					1924:1924	P < 0.05	1922:1929	P < 0.05	1922:1929	The ATTD of InsP6-P was greater (P < 0.05) for HFB than LFB and the ATTD of the sum InsP6-2-P was greater (P < 0.05) for HFB and HFA than LFB.					
35569054	11	36	theme	Total	1958:1962	arg1	release					1972:1978	Total tract P release	1958:1978	Total tract P release	1958:1978	Total tract P release was greater (P < 0.001) for HFB, HFA, and LFW than MFB and LFB.					
35569054	0	37	theme	grower	68:73	arg1	pigs					75:78	grower pigs	68:78	grower pigs	68:78	Cereal grain fiber composition modifies phosphorus digestibility in grower pigs.					
35569054	0	38	theme	fiber	13:17	arg1	composition					19:29	Cereal grain fiber composition	0:29	Cereal grain fiber composition	0:29	Cereal grain fiber composition modifies phosphorus digestibility in grower pigs.					
35569054	6	39	dep	equation	1362:1369	arg1	×					1400:1400	sum InsP6-2-Pdiet (g/kg DM) ×	1372:1400	the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100)	1348:1437	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	14	40	theme	P	2365:2365	arg1	ATTD					2347:2350	ATTD	2347:2350	ATTD	2347:2350	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	14	40	theme	P	2365:2365	arg1	STTD					2357:2360	STTD	2357:2360	STTD	2357:2360	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	6	41	theme	P	1295:1295	arg1	release					1297:1303	The P release	1291:1303	The P release of degraded InsP-P	1291:1322	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	1	42	theme	endogenous	156:165	arg1	losses					167:172	endogenous losses	156:172	endogenous losses including for P	156:188	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	1	42	theme	endogenous	156:165	arg1	P					188:188	P	188:188	P	188:188	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	15	43	theme	Carbohydrate	2415:2426	arg1	fermentation					2428:2439	Carbohydrate fermentation	2415:2439	Carbohydrate fermentation	2415:2439	Carbohydrate fermentation, thus, results in greater total tract P release from InsP-P hydrolysis.					
35569054	13	44	theme	Diet	2096:2099	arg1	content					2110:2116	Diet β-glucan content	2096:2116	Diet β-glucan content	2096:2116	Diet β-glucan content was not correlated with STTD of P (R2 = 0.03) or ATTD of InsP6 (R2 = 0.05).					
35569054	6	45	theme	sum	1372:1374	arg1	×					1400:1400	sum InsP6-2-Pdiet (g/kg DM) ×	1372:1400	the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100)	1348:1437	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	4	46	theme	0.32	1141:1144	arg1	%					1145:1145	%	1145:1145	%	1145:1145	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	3	47	theme	grains	751:756	arg1	HFA					922:924	HFA	922:924	HFA	922:924	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	%					727:727	80%	725:727	80% of one of five cereal grains differing in amylose, β-glucan, and fiber content	725:806	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	MFB					971:973	MFB	971:973	MFB	971:973	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	moderate-fermentable					931:950	3) moderate-fermentable	928:950	3) moderate-fermentable	928:950	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	barley					914:919	hull-less barley	904:919	hull-less barley (HFA)	904:925	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	high-fermentable					872:887	2) high-fermentable	869:887	2) high-fermentable	869:887	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	barley					1004:1009	4) low-fermentable, hulled barley	977:1009	barley	1004:1009	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	high-fermentable					812:827	1) high-fermentable	809:827	1) high-fermentable	809:827	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	grains					751:756	one of five cereal grains	732:756	one of five cereal grains differing in amylose, β-glucan, and fiber content	732:806	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	LFB					1012:1014	LFB	1012:1014	LFB	1012:1014	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	barley					855:860	hull-less barley	845:860	hull-less barley (HFB)	845:866	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	low-fermentable					1025:1039	5) low-fermentable	1022:1039	5) low-fermentable	1022:1039	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	barley					963:968	hull-less barley	953:968	hull-less barley (MFB)	953:974	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	high-amylose					890:901	high-amylose	890:901	high-amylose	890:901	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	high-β-glucan					830:842	high-β-glucan	830:842	high-β-glucan	830:842	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	wheat					1070:1074	Canadian Western Red Spring wheat	1042:1074	Canadian Western Red Spring wheat (LFW)	1042:1080	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	47	theme	grains	751:756	arg1	HFB					863:865	HFB	863:865	HFB	863:865	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	2	48	theme	Ca	473:474	arg1	ATTD					395:398	ATTD	395:398	ATTD	395:398	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	48	theme	Ca	473:474	arg1	digestibility					371:383	apparent ileal digestibility	356:383	apparent ileal digestibility (AID)	356:389	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	0	49	from	digestibility	51:63	arg1	pigs					75:78	grower pigs	68:78	grower pigs	68:78	Cereal grain fiber composition modifies phosphorus digestibility in grower pigs.					
35569054	12	50	theme	Ca	2083:2084	arg1	ATTD					2075:2078	greater (P < 0.05) ATTD	2056:2078	greater (P < 0.05) ATTD of Ca	2056:2084	The LFW had greater (P < 0.05) ATTD of Ca than LFB.					
35569054	3	51	dep	moderate-fermentable	931:950	arg1	3					928:928	3	928:928	3	928:928	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	52	theme	hull-less	904:912	arg1	HFA					922:924	HFA	922:924	HFA	922:924	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	52	theme	hull-less	904:912	arg1	barley					914:919	hull-less barley	904:919	hull-less barley (HFA)	904:925	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	1	53	theme	Increased	81:89	arg1	carbohydrates					103:115	Increased fermentable carbohydrates	81:115	Increased fermentable carbohydrates (e.g., β-glucan, amylose)	81:141	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	10	54	theme	InsP6-2-P	1899:1907	arg1	ATTD					1883:1886	the ATTD	1879:1886	the ATTD of the sum InsP6-2-P	1879:1907	The ATTD of InsP6-P was greater (P < 0.05) for HFB than LFB and the ATTD of the sum InsP6-2-P was greater (P < 0.05) for HFB and HFA than LFB.					
35569054	10	54	theme	InsP6-2-P	1899:1907	arg1	greater					1913:1919	greater	1913:1919	greater	1913:1919	The ATTD of InsP6-P was greater (P < 0.05) for HFB than LFB and the ATTD of the sum InsP6-2-P was greater (P < 0.05) for HFB and HFA than LFB.					
35569054	2	55	theme	apparent	356:363	arg1	digestibility					371:383	apparent ileal digestibility	356:383	apparent ileal digestibility (AID)	356:389	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	0	56	theme	Cereal	0:5	arg1	composition					19:29	Cereal grain fiber composition	0:29	Cereal grain fiber composition	0:29	Cereal grain fiber composition modifies phosphorus digestibility in grower pigs.					
35569054	1	57	theme	P	255:255	arg1	ATTD					246:249	ATTD	246:249	ATTD	246:249	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	1	57	theme	P	255:255	arg1	digestibility					231:243	apparent total tract digestibility	210:243	apparent total tract digestibility (ATTD) of P	210:255	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	15	58	theme	P	2479:2479	arg1	release					2481:2487	greater total tract P release	2459:2487	greater total tract P release from InsP-P hydrolysis	2459:2510	Carbohydrate fermentation, thus, results in greater total tract P release from InsP-P hydrolysis.					
35569054	8	59	theme	0.88	1596:1599	arg1	%					1600:1600	%	1600:1600	%	1600:1600	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	6	60	theme	ATTD	1410:1413	arg1	%					1430:1430	ATTD sum InsP6-2-P (%)/ 100	1410:1436	ATTD sum InsP6-2-P (%)/ 100	1410:1436	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	8	61	dep	%	1653:1653	arg1	to					1655:1656	to	1655:1656	to	1655:1656	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	3	62	dep	high-fermentable	872:887	arg1	2					869:869	2	869:869	2	869:869	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	9	63	dep	MFB	1677:1679	arg1	The					1673:1675	The	1673:1675	The	1673:1675	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	15	64	theme	total	2467:2471	arg1	release					2481:2487	greater total tract P release	2459:2487	greater total tract P release from InsP-P hydrolysis	2459:2510	Carbohydrate fermentation, thus, results in greater total tract P release from InsP-P hydrolysis.					
35569054	2	65	theme	dihydrogen	440:449	arg1	1,2,3,4,5,6-hexakis					419:437	myo-inositol 1,2,3,4,5,6-hexakis	406:437	myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6)	406:467	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	65	theme	dihydrogen	440:449	arg1	phosphate					451:459	dihydrogen phosphate	440:459	dihydrogen phosphate	440:459	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	10	66	theme	P	1922:1922	arg1	<					1924:1924	P < 0.05	1922:1929	P < 0.05	1922:1929	The ATTD of InsP6-P was greater (P < 0.05) for HFB than LFB and the ATTD of the sum InsP6-2-P was greater (P < 0.05) for HFB and HFA than LFB.					
35569054	4	67	theme	0.24	1168:1171	arg1	%					1172:1172	%	1172:1172	%	1172:1172	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	8	68	theme	%	1577:1577	arg1	%					1586:1586	41.4% to 50.6%	1573:1586	41.4% to 50.6% starch	1573:1593	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	7	69	theme	mixed	1467:1471	arg1	effects					1535:1541	random effects	1528:1541	random effects	1528:1541	Data were analyzed using a mixed model with diet as fixed effect, and pig and period as random effects.					
35569054	7	69	theme	mixed	1467:1471	arg1	model					1473:1477	a mixed model	1465:1477	a mixed model with diet	1465:1487	Data were analyzed using a mixed model with diet as fixed effect, and pig and period as random effects.					
35569054	5	70	from	sum	1228:1230	arg1	sample					1283:1288	the sample	1279:1288	the sample	1279:1288	The InsP6-2-P was calculated as the sum of all detected InsP-P (InsP6-P to InsP2-P) in the sample.					
35569054	3	71	theme	fiber	794:798	arg1	content					800:806	fiber content	794:806	fiber content	794:806	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	2	72	theme	1,2,3,4,5,6-hexakis	419:437	arg1	ATTD					395:398	ATTD	395:398	ATTD	395:398	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	72	theme	1,2,3,4,5,6-hexakis	419:437	arg1	digestibility					371:383	apparent ileal digestibility	356:383	apparent ileal digestibility (AID)	356:389	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	13	73	dep	P	2150:2150	arg1	R2					2153:2154	R2	2153:2154	R2 = 0.03	2153:2161	Diet β-glucan content was not correlated with STTD of P (R2 = 0.03) or ATTD of InsP6 (R2 = 0.05).					
35569054	2	74	dep	phosphate	451:459	arg1	InsP6					462:466	InsP6	462:466	InsP6	462:466	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	4	75	dep	%	1154:1154	arg1	P					1162:1162	P	1162:1162	0.32% to 0.53% total P	1141:1162	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	10	76	dep	greater	1839:1845	arg1	<					1850:1850	P < 0.05	1848:1855	P < 0.05	1848:1855	The ATTD of InsP6-P was greater (P < 0.05) for HFB than LFB and the ATTD of the sum InsP6-2-P was greater (P < 0.05) for HFB and HFA than LFB.					
35569054	9	77	dep	greater	1762:1768	arg1	<					1773:1773	P < 0.05	1771:1778	P < 0.05	1771:1778	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	6	78	theme	DM	1396:1397	arg1	×					1400:1400	sum InsP6-2-Pdiet (g/kg DM) ×	1372:1400	the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100)	1348:1437	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	14	79	theme	InsP6-P	2388:2394	arg1	ATTD					2380:2383	greater ATTD	2372:2383	greater ATTD of InsP6-P and sum InsP6-2-P	2372:2412	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	13	80	theme	P	2150:2150	arg1	STTD					2142:2145	STTD	2142:2145	STTD of P (R2 = 0.03)	2142:2162	Diet β-glucan content was not correlated with STTD of P (R2 = 0.03) or ATTD of InsP6 (R2 = 0.05).					
35569054	13	80	theme	P	2150:2150	arg1	ATTD					2167:2170	ATTD	2167:2170	ATTD of InsP6	2167:2179	Diet β-glucan content was not correlated with STTD of P (R2 = 0.03) or ATTD of InsP6 (R2 = 0.05).					
35569054	2	81	theme	cultivars	303:311	arg1	effects					285:291	effects	285:291	effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca	285:474	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	1	82	theme	apparent	210:217	arg1	ATTD					246:249	ATTD	246:249	ATTD	246:249	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	1	82	theme	apparent	210:217	arg1	digestibility					231:243	apparent total tract digestibility	210:243	apparent total tract digestibility (ATTD) of P	210:255	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	12	83	dep	greater	2056:2062	arg1	<					2067:2067	P < 0.05	2065:2072	P < 0.05	2065:2072	The LFW had greater (P < 0.05) ATTD of Ca than LFB.					
35569054	5	84	theme	InsP-P	1248:1253	arg1	sum					1228:1230	the sum	1224:1230	the sum of all detected InsP-P (InsP6-P to InsP2-P) in the sample	1224:1288	The InsP6-2-P was calculated as the sum of all detected InsP-P (InsP6-P to InsP2-P) in the sample.					
35569054	5	84	theme	InsP-P	1248:1253	arg1	InsP6-2-P					1196:1204	The InsP6-2-P	1192:1204	The InsP6-2-P	1192:1204	The InsP6-2-P was calculated as the sum of all detected InsP-P (InsP6-P to InsP2-P) in the sample.					
35569054	13	85	dep	correlated	2126:2135	arg1	R2					2182:2183	R2	2182:2183	R2 = 0.05	2182:2190	Diet β-glucan content was not correlated with STTD of P (R2 = 0.03) or ATTD of InsP6 (R2 = 0.05).					
35569054	2	86	dep	digestibility	371:383	arg1	AID					386:388	AID	386:388	AID	386:388	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	14	87	theme	fermentable	2231:2241	arg1	fiber					2243:2247	fermentable fiber	2231:2247	fermentable fiber	2231:2247	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	1	88	theme	tract	225:229	arg1	ATTD					246:249	ATTD	246:249	ATTD	246:249	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	1	88	theme	tract	225:229	arg1	digestibility					231:243	apparent total tract digestibility	210:243	apparent total tract digestibility (ATTD) of P	210:255	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	3	89	theme	initial	678:684	arg1	BW					686:687	initial BW	678:687	initial BW	678:687	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	89	theme	initial	678:684	arg1	kg					695:696	27.7 kg	690:696	27.7 kg	690:696	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	8	90	theme	0.89	1630:1633	arg1	%					1625:1625	%	1625:1625	%	1625:1625	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	5	91	theme	InsP6-P	1256:1262	arg1	InsP2-P					1267:1273	InsP6-P to InsP2-P	1256:1273	InsP6-P to InsP2-P	1256:1273	The InsP6-2-P was calculated as the sum of all detected InsP-P (InsP6-P to InsP2-P) in the sample.					
35569054	5	91	theme	InsP6-P	1256:1262	arg1	InsP-P					1248:1253	all detected InsP-P	1235:1253	all detected InsP-P (InsP6-P to InsP2-P)	1235:1274	The InsP6-2-P was calculated as the sum of all detected InsP-P (InsP6-P to InsP2-P) in the sample.					
35569054	3	92	theme	period	612:617	arg1	square					638:643	a 6 (period) × 5 (diet) Youden square	607:643	a 6 (period) × 5 (diet) Youden square	607:643	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	14	93	theme	high	2223:2226	arg1	grains					2216:2221	cereal grains	2209:2221	cereal grains high in fermentable fiber	2209:2247	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	3	94	theme	hull-less	953:961	arg1	barley					963:968	hull-less barley	953:968	hull-less barley (MFB)	953:974	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	94	theme	hull-less	953:961	arg1	MFB					971:973	MFB	971:973	MFB	971:973	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	14	95	dep	amylose	2256:2262	arg1	e.g.					2250:2253	e.g.	2250:2253	e.g.	2250:2253	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	8	96	theme	total	1636:1640	arg1	P					1642:1642	total P	1636:1642	0.81% to 0.89% total P	1621:1642	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	2	97	theme	present	262:268	arg1	study					270:274	The present study	258:274	The present study	258:274	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	14	98	theme	cereal	2209:2214	arg1	grains					2216:2221	cereal grains	2209:2221	cereal grains high in fermentable fiber	2209:2247	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	2	99	theme	P	530:530	arg1	presence					540:547	the presence	536:547	the presence of lower inositol phosphates (InsP)	536:583	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	99	theme	P	530:530	arg1	digestibility					506:518	standardized total tract digestibility	481:518	standardized total tract digestibility (STTD) of P	481:530	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	99	theme	P	530:530	arg1	STTD					521:524	STTD	521:524	STTD	521:524	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	6	100	theme	InsP6-2-P	1419:1427	arg1	%					1430:1430	ATTD sum InsP6-2-P (%)/ 100	1410:1436	ATTD sum InsP6-2-P (%)/ 100	1410:1436	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	3	101	dep	barley	1004:1009	arg1	4					977:977	4	977:977	4	977:977	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	2	102	theme	inositol	558:565	arg1	InsP					579:582	InsP	579:582	InsP	579:582	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	102	theme	inositol	558:565	arg1	phosphates					567:576	lower inositol phosphates	552:576	lower inositol phosphates (InsP)	552:583	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	103	from	starch	336:341	arg1	ATTD					395:398	ATTD	395:398	ATTD	395:398	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	103	from	starch	336:341	arg1	digestibility					371:383	apparent ileal digestibility	356:383	apparent ileal digestibility (AID)	356:389	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	8	104	theme	0.19	1649:1652	arg1	%					1653:1653	%	1653:1653	%	1653:1653	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	3	105	theme	×	620:620	arg1	square					638:643	a 6 (period) × 5 (diet) Youden square	607:643	a 6 (period) × 5 (diet) Youden square	607:643	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	8	106	dep	%	1600:1600	arg1	to					1602:1603	to	1602:1603	to	1602:1603	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	3	107	theme	hulled	997:1002	arg1	LFB					1012:1014	LFB	1012:1014	LFB	1012:1014	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	107	theme	hulled	997:1002	arg1	barley					1004:1009	4) low-fermentable, hulled barley	977:1009	barley	1004:1009	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	2	108	from	fiber	347:351	arg1	ATTD					395:398	ATTD	395:398	ATTD	395:398	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	108	from	fiber	347:351	arg1	digestibility					371:383	apparent ileal digestibility	356:383	apparent ileal digestibility (AID)	356:389	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	3	109	from	diets	708:712	arg1	square					638:643	a 6 (period) × 5 (diet) Youden square	607:643	a 6 (period) × 5 (diet) Youden square	607:643	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	14	110	theme	hull-less	2299:2307	arg1	cultivars					2316:2324	specific hull-less barley cultivars	2290:2324	specific hull-less barley cultivars	2290:2324	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	3	111	theme	low-fermentable	980:994	arg1	LFB					1012:1014	LFB	1012:1014	LFB	1012:1014	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	111	theme	low-fermentable	980:994	arg1	barley					1004:1009	4) low-fermentable, hulled barley	977:1009	barley	1004:1009	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	13	112	theme	InsP6	2175:2179	arg1	STTD					2142:2145	STTD	2142:2145	STTD of P (R2 = 0.03)	2142:2162	Diet β-glucan content was not correlated with STTD of P (R2 = 0.03) or ATTD of InsP6 (R2 = 0.05).					
35569054	13	112	theme	InsP6	2175:2179	arg1	ATTD					2167:2170	ATTD	2167:2170	ATTD of InsP6	2167:2179	Diet β-glucan content was not correlated with STTD of P (R2 = 0.03) or ATTD of InsP6 (R2 = 0.05).					
35569054	2	113	theme	tract	500:504	arg1	STTD					521:524	STTD	521:524	STTD	521:524	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	113	theme	tract	500:504	arg1	digestibility					506:518	standardized total tract digestibility	481:518	standardized total tract digestibility (STTD) of P	481:530	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	14	114	from	high	2223:2226	arg1	fiber					2243:2247	fermentable fiber	2231:2247	fermentable fiber	2231:2247	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	3	115	theme	Western	1051:1057	arg1	wheat					1070:1074	Canadian Western Red Spring wheat	1042:1074	Canadian Western Red Spring wheat (LFW)	1042:1080	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	115	theme	Western	1051:1057	arg1	grains					751:756	one of five cereal grains	732:756	one of five cereal grains differing in amylose, β-glucan, and fiber content	732:806	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	115	theme	Western	1051:1057	arg1	%					727:727	80%	725:727	80% of one of five cereal grains differing in amylose, β-glucan, and fiber content	725:806	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	115	theme	Western	1051:1057	arg1	LFW					1077:1079	LFW	1077:1079	LFW	1077:1079	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	116	dep	high-fermentable	812:827	arg1	1					809:809	1	809:809	1	809:809	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	13	117	theme	=	2156:2156	arg1	R2					2153:2154	R2	2153:2154	R2 = 0.03	2153:2161	Diet β-glucan content was not correlated with STTD of P (R2 = 0.03) or ATTD of InsP6 (R2 = 0.05).					
35569054	2	118	theme	standardized	481:492	arg1	STTD					521:524	STTD	521:524	STTD	521:524	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	118	theme	standardized	481:492	arg1	digestibility					506:518	standardized total tract digestibility	481:518	standardized total tract digestibility (STTD) of P	481:530	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	9	119	contain	had	1695:1697	arg1	MFB					1677:1679	MFB	1677:1679	MFB	1677:1679	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	9	119	contain	had	1695:1697	arg2	diet					1718:1721	greater (P < 0.05) diet	1699:1721	greater (P < 0.05) diet AID of P	1699:1730	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	9	119	contain	had	1695:1697	arg1	LFB					1682:1684	LFB	1682:1684	LFB	1682:1684	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	9	119	contain	had	1695:1697	arg1	LFW					1691:1693	LFW	1691:1693	LFW	1691:1693	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	11	120	theme	P	1993:1993	arg1	<					1995:1995	P < 0.001	1993:2001	P < 0.001	1993:2001	Total tract P release was greater (P < 0.001) for HFB, HFA, and LFW than MFB and LFB.					
35569054	4	121	theme	dry	1086:1088	arg1	basis					1102:1106	dry matter (DM) basis	1086:1106	dry matter (DM) basis	1086:1106	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	8	122	dep	%	1634:1634	arg1	P					1642:1642	total P	1636:1642	0.81% to 0.89% total P	1621:1642	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	14	123	theme	AID	2342:2344	arg1	diet					2337:2340	lower diet	2331:2340	lower diet AID	2331:2344	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	14	124	from	fiber	2243:2247	arg1	high					2223:2226	high	2223:2226	high	2223:2226	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	6	125	theme	InsP-P	1317:1322	arg1	release					1297:1303	The P release	1291:1303	The P release of degraded InsP-P	1291:1322	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	9	126	dep	greater	1699:1705	arg1	<					1710:1710	P < 0.05	1708:1715	P < 0.05	1708:1715	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	13	127	theme	β-glucan	2101:2108	arg1	content					2110:2116	Diet β-glucan content	2096:2116	Diet β-glucan content	2096:2116	Diet β-glucan content was not correlated with STTD of P (R2 = 0.03) or ATTD of InsP6 (R2 = 0.05).					
35569054	14	128	theme	lower	2331:2335	arg1	diet					2337:2340	lower diet	2331:2340	lower diet AID	2331:2344	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	15	129	theme	greater	2459:2465	arg1	release					2481:2487	greater total tract P release	2459:2487	greater total tract P release from InsP-P hydrolysis	2459:2510	Carbohydrate fermentation, thus, results in greater total tract P release from InsP-P hydrolysis.					
35569054	9	130	dep	diet	1718:1721	arg1	AID					1723:1725	AID	1723:1725	AID of P	1723:1730	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	3	131	theme	Spring	1063:1068	arg1	wheat					1070:1074	Canadian Western Red Spring wheat	1042:1074	Canadian Western Red Spring wheat (LFW)	1042:1080	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	131	theme	Spring	1063:1068	arg1	grains					751:756	one of five cereal grains	732:756	one of five cereal grains differing in amylose, β-glucan, and fiber content	732:806	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	131	theme	Spring	1063:1068	arg1	%					727:727	80%	725:727	80% of one of five cereal grains differing in amylose, β-glucan, and fiber content	725:806	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	131	theme	Spring	1063:1068	arg1	LFW					1077:1079	LFW	1077:1079	LFW	1077:1079	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	9	132	theme	P	1803:1803	arg1	ATTD					1786:1789	greater (P < 0.05) diet ATTD	1762:1789	greater (P < 0.05) diet ATTD	1762:1789	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	9	132	theme	P	1803:1803	arg1	STTD					1795:1798	STTD	1795:1798	STTD	1795:1798	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	9	133	contain	had	1758:1760	arg2	STTD					1795:1798	STTD	1795:1798	STTD	1795:1798	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	9	133	contain	had	1758:1760	arg1	MFB					1754:1756	MFB	1754:1756	MFB	1754:1756	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	9	133	contain	had	1758:1760	arg2	ATTD					1786:1789	greater (P < 0.05) diet ATTD	1762:1789	greater (P < 0.05) diet ATTD	1762:1789	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	6	134	theme	g/kg	1391:1394	arg1	×					1400:1400	sum InsP6-2-Pdiet (g/kg DM) ×	1372:1400	the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100)	1348:1437	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	4	135	dep	%	1172:1172	arg1	to					1174:1175	to	1174:1175	to	1174:1175	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	11	136	theme	tract	1964:1968	arg1	release					1972:1978	Total tract P release	1958:1978	Total tract P release	1958:1978	Total tract P release was greater (P < 0.001) for HFB, HFA, and LFW than MFB and LFB.					
35569054	4	137	theme	0.53	1150:1153	arg1	%					1145:1145	%	1145:1145	%	1145:1145	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	3	138	theme	diet	625:628	arg1	square					638:643	a 6 (period) × 5 (diet) Youden square	607:643	a 6 (period) × 5 (diet) Youden square	607:643	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	14	139	contain	had	2327:2329	arg2	ATTD					2347:2350	ATTD	2347:2350	ATTD	2347:2350	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	14	139	contain	had	2327:2329	arg2	diet					2337:2340	lower diet	2331:2340	lower diet AID	2331:2344	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	14	139	contain	had	2327:2329	arg1	grains					2216:2221	cereal grains	2209:2221	cereal grains high in fermentable fiber	2209:2247	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	14	139	contain	had	2327:2329	arg2	ATTD					2380:2383	greater ATTD	2372:2383	greater ATTD of InsP6-P and sum InsP6-2-P	2372:2412	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	6	140	theme	InsP6-2-Pdiet	1376:1388	arg1	×					1400:1400	sum InsP6-2-Pdiet (g/kg DM) ×	1372:1400	the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100)	1348:1437	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	1	141	dep	carbohydrates	103:115	arg1	amylose					134:140	amylose	134:140	amylose	134:140	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	1	141	dep	carbohydrates	103:115	arg1	β-glucan					124:131	β-glucan	124:131	β-glucan	124:131	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	14	142	theme	barley	2309:2314	arg1	cultivars					2316:2324	specific hull-less barley cultivars	2290:2324	specific hull-less barley cultivars	2290:2324	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	14	143	theme	specific	2290:2297	arg1	cultivars					2316:2324	specific hull-less barley cultivars	2290:2324	specific hull-less barley cultivars	2290:2324	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	13	144	dep	=	2185:2185	arg1	0.05					2187:2190	0.05	2187:2190	0.05	2187:2190	Diet β-glucan content was not correlated with STTD of P (R2 = 0.03) or ATTD of InsP6 (R2 = 0.05).					
35569054	7	145	theme	fixed	1492:1496	arg1	effect					1498:1503	fixed effect	1492:1503	fixed effect	1492:1503	Data were analyzed using a mixed model with diet as fixed effect, and pig and period as random effects.					
35569054	6	146	theme	following	1352:1360	arg1	equation					1362:1369	the following equation	1348:1369	the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100)	1348:1437	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	1	147	dep	β-glucan	124:131	arg1	e.g.					118:121	e.g.	118:121	e.g.	118:121	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	2	148	theme	ileal	365:369	arg1	digestibility					371:383	apparent ileal digestibility	356:383	apparent ileal digestibility (AID)	356:389	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	0	149	theme	grain	7:11	arg1	composition					19:29	Cereal grain fiber composition	0:29	Cereal grain fiber composition	0:29	Cereal grain fiber composition modifies phosphorus digestibility in grower pigs.					
35569054	3	150	dep	barrows	669:675	arg1	BW					686:687	initial BW	678:687	initial BW	678:687	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	3	150	dep	barrows	669:675	arg1	kg					695:696	27.7 kg	690:696	27.7 kg	690:696	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	5	151	dep	InsP2-P	1267:1273	arg1	to					1264:1265	to	1264:1265	to	1264:1265	The InsP6-2-P was calculated as the sum of all detected InsP-P (InsP6-P to InsP2-P) in the sample.					
35569054	1	152	theme	fermentable	91:101	arg1	carbohydrates					103:115	Increased fermentable carbohydrates	81:115	Increased fermentable carbohydrates (e.g., β-glucan, amylose)	81:141	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	3	153	theme	cereal	744:749	arg1	grains					751:756	one of five cereal grains	732:756	one of five cereal grains differing in amylose, β-glucan, and fiber content	732:806	In a 6 (period) × 5 (diet) Youden square, seven ileal-cannulated barrows (initial BW, 27.7 kg) were fed diets containing 80% of one of five cereal grains differing in amylose, β-glucan, and fiber content: 1) high-fermentable, high-β-glucan, hull-less barley (HFB); 2) high-fermentable, high-amylose, hull-less barley (HFA); 3) moderate-fermentable, hull-less barley (MFB); 4) low-fermentable, hulled barley (LFB); and 5) low-fermentable, Canadian Western Red Spring wheat (LFW).					
35569054	2	154	theme	P	403:403	arg1	ATTD					395:398	ATTD	395:398	ATTD	395:398	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	154	theme	P	403:403	arg1	digestibility					371:383	apparent ileal digestibility	356:383	apparent ileal digestibility (AID)	356:389	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	6	155	theme	sum	1415:1417	arg1	%					1430:1430	ATTD sum InsP6-2-P (%)/ 100	1410:1436	ATTD sum InsP6-2-P (%)/ 100	1410:1436	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	10	156	theme	sum	1895:1897	arg1	InsP6-2-P					1899:1907	the sum InsP6-2-P	1891:1907	the sum InsP6-2-P	1891:1907	The ATTD of InsP6-P was greater (P < 0.05) for HFB than LFB and the ATTD of the sum InsP6-2-P was greater (P < 0.05) for HFB and HFA than LFB.					
35569054	4	157	theme	0.50	1177:1180	arg1	%					1172:1172	%	1172:1172	%	1172:1172	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	8	158	theme	8.54	1605:1608	arg1	%					1600:1600	%	1600:1600	%	1600:1600	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	4	159	theme	total	1156:1160	arg1	%					1154:1154	0.32% to 0.53% total	1141:1160	0.32% to 0.53% total P	1141:1162	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	0	160	theme	phosphorus	40:49	arg1	digestibility					51:63	phosphorus digestibility	40:63	phosphorus digestibility in grower pigs	40:78	Cereal grain fiber composition modifies phosphorus digestibility in grower pigs.					
35569054	10	161	theme	InsP6-P	1827:1833	arg1	ATTD					1819:1822	The ATTD	1815:1822	The ATTD of InsP6-P	1815:1833	The ATTD of InsP6-P was greater (P < 0.05) for HFB than LFB and the ATTD of the sum InsP6-2-P was greater (P < 0.05) for HFB and HFA than LFB.					
35569054	10	161	theme	InsP6-P	1827:1833	arg1	greater					1839:1845	greater	1839:1845	greater	1839:1845	The ATTD of InsP6-P was greater (P < 0.05) for HFB than LFB and the ATTD of the sum InsP6-2-P was greater (P < 0.05) for HFB and HFA than LFB.					
35569054	15	162	theme	tract	2473:2477	arg1	release					2481:2487	greater total tract P release	2459:2487	greater total tract P release from InsP-P hydrolysis	2459:2510	Carbohydrate fermentation, thus, results in greater total tract P release from InsP-P hydrolysis.					
35569054	8	163	theme	DM	1547:1548	arg1	basis					1550:1554	DM basis	1547:1554	DM basis	1547:1554	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	4	164	theme	matter	1090:1095	arg1	basis					1102:1106	dry matter (DM) basis	1086:1106	dry matter (DM) basis	1086:1106	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	6	165	dep	×	1400:1400	arg1	%					1430:1430	ATTD sum InsP6-2-P (%)/ 100	1410:1436	ATTD sum InsP6-2-P (%)/ 100	1410:1436	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	6	165	dep	×	1400:1400	arg1	AID					1403:1405	AID	1403:1405	AID	1403:1405	The P release of degraded InsP-P was calculated by using the following equation: sum InsP6-2-Pdiet (g/kg DM) × (AID or ATTD sum InsP6-2-P (%)/ 100).					
35569054	8	166	theme	0.81	1621:1624	arg1	%					1625:1625	%	1625:1625	%	1625:1625	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	2	167	theme	myo-inositol	406:417	arg1	1,2,3,4,5,6-hexakis					419:437	myo-inositol 1,2,3,4,5,6-hexakis	406:437	myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6)	406:467	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	2	167	theme	myo-inositol	406:417	arg1	phosphate					451:459	dihydrogen phosphate	440:459	dihydrogen phosphate	440:459	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	13	168	theme	=	2185:2185	arg1	R2					2182:2183	R2	2182:2183	R2 = 0.05	2182:2190	Diet β-glucan content was not correlated with STTD of P (R2 = 0.03) or ATTD of InsP6 (R2 = 0.05).					
35569054	4	169	contain	contained	1123:1131	arg1	basis					1102:1106	dry matter (DM) basis	1086:1106	dry matter (DM) basis	1086:1106	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	4	169	contain	contained	1123:1131	arg2	grains					1116:1121	cereal grains	1109:1121	cereal grains	1109:1121	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	14	170	theme	sum	2400:2402	arg1	InsP6-2-P					2404:2412	sum InsP6-2-P	2400:2412	sum InsP6-2-P	2400:2412	In conclusion, cereal grains high in fermentable fiber, e.g., amylose and β-glucans included in specific hull-less barley cultivars, had lower diet AID, ATTD, and STTD of P, but greater ATTD of InsP6-P and sum InsP6-2-P.					
35569054	9	171	theme	greater	1699:1705	arg1	diet					1718:1721	greater (P < 0.05) diet	1699:1721	greater (P < 0.05) diet AID of P	1699:1730	The MFB, LFB, and LFW had greater (P < 0.05) diet AID of P than HFB and HFA, and MFB had greater (P < 0.05) diet ATTD and STTD of P than HFB.					
35569054	8	172	contain	contained	1563:1571	arg2	%					1586:1586	41.4% to 50.6%	1573:1586	41.4% to 50.6% starch	1573:1593	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	8	172	contain	contained	1563:1571	arg2	%					1634:1634	0.81% to 0.89%	1621:1634	0.81% to 0.89% total P	1621:1642	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	8	172	contain	contained	1563:1571	arg2	%					1609:1609	0.88% to 8.54%	1596:1609	0.88% to 8.54% β-glucan	1596:1618	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	8	172	contain	contained	1563:1571	arg2	diets					1557:1561	diets	1557:1561	diets	1557:1561	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	8	172	contain	contained	1563:1571	arg1	basis					1550:1554	DM basis	1547:1554	DM basis	1547:1554	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	8	172	contain	contained	1563:1571	arg2	%					1662:1662	0.19% to 0.35%	1649:1662	0.19% to 0.35% InsP6-P	1649:1670	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	8	172	contain	contained	1563:1571	arg1	diets					1557:1561	diets	1557:1561	diets	1557:1561	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	2	173	theme	barley	296:301	arg1	cultivars					303:311	barley cultivars	296:311	barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca	296:474	The present study assessed effects of barley cultivars varying in fermentable starch and fiber on apparent ileal digestibility (AID) and ATTD of P, myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate; InsP6) and Ca, and standardized total tract digestibility (STTD) of P and the presence of lower inositol phosphates (InsP) compared to wheat.					
35569054	1	174	theme	total	219:223	arg1	ATTD					246:249	ATTD	246:249	ATTD	246:249	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	1	174	theme	total	219:223	arg1	digestibility					231:243	apparent total tract digestibility	210:243	apparent total tract digestibility (ATTD) of P	210:255	Increased fermentable carbohydrates (e.g., β-glucan, amylose) may increase endogenous losses including for P, and thereby reduce apparent total tract digestibility (ATTD) of P.					
35569054	4	175	dep	%	1145:1145	arg1	to					1147:1148	to	1147:1148	to	1147:1148	On dry matter (DM) basis, cereal grains contained between 0.32% to 0.53% total P and 0.24% to 0.50% InsP6-P.					
35569054	8	176	dep	%	1662:1662	arg1	InsP6-P					1664:1670	InsP6-P	1664:1670	0.19% to 0.35% InsP6-P	1649:1670	On DM basis, diets contained 41.4% to 50.6% starch, 0.88% to 8.54% β-glucan, 0.81% to 0.89% total P, and 0.19% to 0.35% InsP6-P.					
35569054	12	177	theme	greater	2056:2062	arg1	ATTD					2075:2078	greater (P < 0.05) ATTD	2056:2078	greater (P < 0.05) ATTD of Ca	2056:2084	The LFW had greater (P < 0.05) ATTD of Ca than LFB.					
35269503	0	0	theme	Cell-Adhesive	100:112	arg1	Hydrogels					114:122	Cell-Adhesive Hydrogels	100:122	Cell-Adhesive Hydrogels	100:122	Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.					
35269503	2	1	theme	polymers	520:527	arg1	degradation					501:511	degradation	501:511	degradation	501:511	Here, we investigated the possibility of regulating cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air.					
35269503	2	1	theme	polymers	520:527	arg1	cross-linking					483:495	hydrogen peroxide (H2O2)-mediated cross-linking	449:495	hydrogen peroxide (H2O2)-mediated cross-linking	449:495	Here, we investigated the possibility of regulating cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air.					
35269503	5	2	theme	HeLa/Fucci2	867:877	arg1	cells					879:883	HeLa/Fucci2 cells	867:883	HeLa/Fucci2 cells cultured on the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure	867:1007	Although HeLa/Fucci2 cells cultured on the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure showed a G2/M-phase arrest, NMuMG cells showed a G1-phase arrest.					
35269503	4	3	theme	gland	702:706	arg1	cells					719:723	mouse mammary gland epithelial cells	688:723	mouse mammary gland epithelial cells (NMuMG)	688:731	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	4	3	theme	gland	702:706	arg1	NMuMG					726:730	NMuMG	726:730	NMuMG	726:730	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	1	4	theme	cells	261:265	arg1	interaction					246:256	interaction	246:256	interaction of cells with the adhering substrates	246:294	The cell cycle is known to be regulated by features such as the mechanical properties of the surrounding environment and interaction of cells with the adhering substrates.					
35269503	1	4	theme	cells	261:265	arg1	properties					200:209	the mechanical properties	185:209	the mechanical properties of the surrounding environment	185:240	The cell cycle is known to be regulated by features such as the mechanical properties of the surrounding environment and interaction of cells with the adhering substrates.					
35269503	4	5	theme	mammary	694:700	arg1	cells					719:723	mouse mammary gland epithelial cells	688:723	mouse mammary gland epithelial cells (NMuMG)	688:731	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	4	5	theme	mammary	694:700	arg1	NMuMG					726:730	NMuMG	726:730	NMuMG	726:730	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	6	6	theme	low-molecular-weight	1196:1215	arg1	HA					1217:1218	the low-molecular-weight HA	1192:1218	the low-molecular-weight HA resulting from H2O2-mediated degradation	1192:1259	Additionally, the cell-cycle progression of NMuMG cells was not only governed by the hydrogel stiffness, but also by the low-molecular-weight HA resulting from H2O2-mediated degradation.					
35269503	4	7	theme	cell-cycle	744:753	arg1	Fucci2					764:769	cell-cycle reporter Fucci2	744:769	cell-cycle reporter Fucci2	744:769	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	0	8	theme	Hydrogels	114:122	arg1	Degradation					85:95	Degradation	85:95	Degradation of Cell-Adhesive Hydrogels	85:122	Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.					
35269503	0	8	theme	Hydrogels	114:122	arg1	Cross-Linking					67:79	Hydrogen Peroxide-Mediated Cross-Linking	40:79	Hydrogen Peroxide-Mediated Cross-Linking	40:79	Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.					
35269503	2	9	from	progression	360:370	arg1	hydrogels					422:430	gelatin/hyaluronic acid composite hydrogels	388:430	gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air	388:585	Here, we investigated the possibility of regulating cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air.					
35269503	6	10	theme	hydrogel	1160:1167	arg1	stiffness					1169:1177	the hydrogel stiffness	1156:1177	the hydrogel stiffness	1156:1177	Additionally, the cell-cycle progression of NMuMG cells was not only governed by the hydrogel stiffness, but also by the low-molecular-weight HA resulting from H2O2-mediated degradation.					
35269503	1	11	theme	adhering	276:283	arg1	substrates					285:294	the adhering substrates	272:294	the adhering substrates	272:294	The cell cycle is known to be regulated by features such as the mechanical properties of the surrounding environment and interaction of cells with the adhering substrates.					
35269503	2	12	theme	exposure	544:551	arg1	time					553:556	the exposure time	540:556	the exposure time to H2O2 contained in the air	540:585	Here, we investigated the possibility of regulating cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air.					
35269503	4	13	theme	exposure-time-dependent	782:804	arg1	progressions					827:838	the exposure-time-dependent different cell-cycle progressions	778:838	the exposure-time-dependent different cell-cycle progressions on the hydrogels	778:855	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	6	14	theme	NMuMG	1119:1123	arg1	cells					1125:1129	NMuMG cells	1119:1129	NMuMG cells	1119:1129	Additionally, the cell-cycle progression of NMuMG cells was not only governed by the hydrogel stiffness, but also by the low-molecular-weight HA resulting from H2O2-mediated degradation.					
35269503	2	15	theme	composite	412:420	arg1	hydrogels					422:430	gelatin/hyaluronic acid composite hydrogels	388:430	gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air	388:585	Here, we investigated the possibility of regulating cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air.					
35269503	2	16	theme	-mediated	473:481	arg1	cross-linking					483:495	hydrogen peroxide (H2O2)-mediated cross-linking	449:495	hydrogen peroxide (H2O2)-mediated cross-linking	449:495	Here, we investigated the possibility of regulating cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air.					
35269503	6	17	theme	H2O2-mediated	1235:1247	arg1	degradation					1249:1259	H2O2-mediated degradation	1235:1259	H2O2-mediated degradation	1235:1259	Additionally, the cell-cycle progression of NMuMG cells was not only governed by the hydrogel stiffness, but also by the low-molecular-weight HA resulting from H2O2-mediated degradation.					
35269503	5	18	dep	modulus	924:930	arg1	kPa					947:949	0.20 and 0.40 kPa	933:949	Young's modulus: 0.20 and 0.40 kPa	916:949	Although HeLa/Fucci2 cells cultured on the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure showed a G2/M-phase arrest, NMuMG cells showed a G1-phase arrest.					
35269503	2	19	theme	acid	407:410	arg1	hydrogels					422:430	gelatin/hyaluronic acid composite hydrogels	388:430	gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air	388:585	Here, we investigated the possibility of regulating cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air.					
35269503	7	20	theme	H2O2-mediated	1290:1302	arg1	cross-linking					1304:1316	H2O2-mediated cross-linking	1290:1316	H2O2-mediated cross-linking	1290:1316	These results indicate that H2O2-mediated cross-linking and degradation of gelatin/hyaluronic acid composite hydrogel could be used to control the cell adhesion and cell-cycle progression.					
35269503	2	21	theme	gelatin/hyaluronic	388:405	arg1	hydrogels					422:430	gelatin/hyaluronic acid composite hydrogels	388:430	gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air	388:585	Here, we investigated the possibility of regulating cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air.					
35269503	0	22	theme	Progression	25:35	arg1	Modulation					0:9	Modulation	0:9	Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.	0:123	Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.					
35269503	7	23	theme	hydrogel	1371:1378	arg1	degradation					1322:1332	degradation	1322:1332	degradation	1322:1332	These results indicate that H2O2-mediated cross-linking and degradation of gelatin/hyaluronic acid composite hydrogel could be used to control the cell adhesion and cell-cycle progression.					
35269503	7	23	theme	hydrogel	1371:1378	arg1	cross-linking					1304:1316	H2O2-mediated cross-linking	1290:1316	H2O2-mediated cross-linking	1290:1316	These results indicate that H2O2-mediated cross-linking and degradation of gelatin/hyaluronic acid composite hydrogel could be used to control the cell adhesion and cell-cycle progression.					
35269503	4	24	theme	reporter	755:762	arg1	Fucci2					764:769	cell-cycle reporter Fucci2	744:769	cell-cycle reporter Fucci2	744:769	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	5	25	dep	hydrogel	906:913	arg1	modulus					924:930	Young's modulus	916:930	Young's modulus: 0.20 and 0.40 kPa	916:949	Although HeLa/Fucci2 cells cultured on the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure showed a G2/M-phase arrest, NMuMG cells showed a G1-phase arrest.					
35269503	4	26	theme	Human	649:653	arg1	HeLa					678:681	HeLa	678:681	HeLa	678:681	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	4	26	theme	Human	649:653	arg1	cells					671:675	Human cervical cancer cells	649:675	Human cervical cancer cells (HeLa)	649:682	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	0	27	theme	Cell-Cycle	14:23	arg1	Progression					25:35	Cell-Cycle Progression	14:35	Cell-Cycle Progression	14:35	Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.					
35269503	1	28	theme	cell	129:132	arg1	cycle					134:138	The cell cycle	125:138	The cell cycle	125:138	The cell cycle is known to be regulated by features such as the mechanical properties of the surrounding environment and interaction of cells with the adhering substrates.					
35269503	7	29	theme	cell-cycle	1427:1436	arg1	progression					1438:1448	cell-cycle progression	1427:1448	cell-cycle progression	1427:1448	These results indicate that H2O2-mediated cross-linking and degradation of gelatin/hyaluronic acid composite hydrogel could be used to control the cell adhesion and cell-cycle progression.					
35269503	2	30	theme	hydrogen	449:456	arg1	H2O2					468:471	H2O2	468:471	H2O2	468:471	Here, we investigated the possibility of regulating cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air.					
35269503	2	30	theme	hydrogen	449:456	arg1	peroxide					458:465	hydrogen peroxide	449:465	hydrogen peroxide (H2O2)	449:472	Here, we investigated the possibility of regulating cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air.					
35269503	0	31	theme	Hydrogen	40:47	arg1	Cross-Linking					67:79	Hydrogen Peroxide-Mediated Cross-Linking	40:79	Hydrogen Peroxide-Mediated Cross-Linking	40:79	Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.					
35269503	4	32	theme	epithelial	708:717	arg1	cells					719:723	mouse mammary gland epithelial cells	688:723	mouse mammary gland epithelial cells (NMuMG)	688:731	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	4	32	theme	epithelial	708:717	arg1	NMuMG					726:730	NMuMG	726:730	NMuMG	726:730	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	1	33	with	properties	200:209	arg1	substrates					285:294	the adhering substrates	272:294	the adhering substrates	272:294	The cell cycle is known to be regulated by features such as the mechanical properties of the surrounding environment and interaction of cells with the adhering substrates.					
35269503	5	34	theme	G1-phase	1058:1065	arg1	arrest					1067:1072	a G1-phase arrest	1056:1072	a G1-phase arrest	1056:1072	Although HeLa/Fucci2 cells cultured on the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure showed a G2/M-phase arrest, NMuMG cells showed a G1-phase arrest.					
35269503	3	35	theme	hydrogel	609:616	arg1	stiffness					592:600	The stiffness	588:600	The stiffness of the hydrogel	588:616	The stiffness of the hydrogel varied with the exposure time.					
35269503	1	36	theme	mechanical	189:198	arg1	properties					200:209	the mechanical properties	185:209	the mechanical properties of the surrounding environment	185:240	The cell cycle is known to be regulated by features such as the mechanical properties of the surrounding environment and interaction of cells with the adhering substrates.					
35269503	4	37	theme	cancer	664:669	arg1	HeLa					678:681	HeLa	678:681	HeLa	678:681	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	4	37	theme	cancer	664:669	arg1	cells					671:675	Human cervical cancer cells	649:675	Human cervical cancer cells (HeLa)	649:682	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	7	38	theme	acid	1356:1359	arg1	hydrogel					1371:1378	gelatin/hyaluronic acid composite hydrogel	1337:1378	gelatin/hyaluronic acid composite hydrogel	1337:1378	These results indicate that H2O2-mediated cross-linking and degradation of gelatin/hyaluronic acid composite hydrogel could be used to control the cell adhesion and cell-cycle progression.					
35269503	7	39	theme	cell	1409:1412	arg1	adhesion					1414:1421	the cell adhesion	1405:1421	the cell adhesion	1405:1421	These results indicate that H2O2-mediated cross-linking and degradation of gelatin/hyaluronic acid composite hydrogel could be used to control the cell adhesion and cell-cycle progression.					
35269503	1	40	theme	surrounding	218:228	arg1	environment					230:240	the surrounding environment	214:240	the surrounding environment	214:240	The cell cycle is known to be regulated by features such as the mechanical properties of the surrounding environment and interaction of cells with the adhering substrates.					
35269503	5	41	theme	Young	916:920	arg1	modulus					924:930	Young's modulus	916:930	Young's modulus: 0.20 and 0.40 kPa	916:949	Although HeLa/Fucci2 cells cultured on the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure showed a G2/M-phase arrest, NMuMG cells showed a G1-phase arrest.					
35269503	5	42	theme	G2/M-phase	1018:1027	arg1	arrest					1029:1034	a G2/M-phase arrest	1016:1034	a G2/M-phase arrest	1016:1034	Although HeLa/Fucci2 cells cultured on the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure showed a G2/M-phase arrest, NMuMG cells showed a G1-phase arrest.					
35269503	6	43	theme	cell-cycle	1093:1102	arg1	progression					1104:1114	the cell-cycle progression	1089:1114	the cell-cycle progression of NMuMG cells	1089:1129	Additionally, the cell-cycle progression of NMuMG cells was not only governed by the hydrogel stiffness, but also by the low-molecular-weight HA resulting from H2O2-mediated degradation.					
35269503	6	44	theme	cells	1125:1129	arg1	progression					1104:1114	the cell-cycle progression	1089:1114	the cell-cycle progression of NMuMG cells	1089:1129	Additionally, the cell-cycle progression of NMuMG cells was not only governed by the hydrogel stiffness, but also by the low-molecular-weight HA resulting from H2O2-mediated degradation.					
35269503	0	45	theme	Peroxide-Mediated	49:65	arg1	Cross-Linking					67:79	Hydrogen Peroxide-Mediated Cross-Linking	40:79	Hydrogen Peroxide-Mediated Cross-Linking	40:79	Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.					
35269503	7	46	theme	gelatin/hyaluronic	1337:1354	arg1	hydrogel					1371:1378	gelatin/hyaluronic acid composite hydrogel	1337:1378	gelatin/hyaluronic acid composite hydrogel	1337:1378	These results indicate that H2O2-mediated cross-linking and degradation of gelatin/hyaluronic acid composite hydrogel could be used to control the cell adhesion and cell-cycle progression.					
35269503	5	47	theme	NMuMG	1037:1041	arg1	cells					1043:1047	NMuMG cells	1037:1047	NMuMG cells	1037:1047	Although HeLa/Fucci2 cells cultured on the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure showed a G2/M-phase arrest, NMuMG cells showed a G1-phase arrest.					
35269503	7	48	theme	composite	1361:1369	arg1	hydrogel					1371:1378	gelatin/hyaluronic acid composite hydrogel	1337:1378	gelatin/hyaluronic acid composite hydrogel	1337:1378	These results indicate that H2O2-mediated cross-linking and degradation of gelatin/hyaluronic acid composite hydrogel could be used to control the cell adhesion and cell-cycle progression.					
35269503	2	49	theme	cell-cycle	349:358	arg1	progression					360:370	cell-cycle progression	349:370	cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air	349:585	Here, we investigated the possibility of regulating cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air.					
35269503	7	50	used	used	1389:1392	arg2	degradation					1322:1332	degradation	1322:1332	degradation	1322:1332	These results indicate that H2O2-mediated cross-linking and degradation of gelatin/hyaluronic acid composite hydrogel could be used to control the cell adhesion and cell-cycle progression.					
35269503	7	50	used	used	1389:1392	arg2	cross-linking					1304:1316	H2O2-mediated cross-linking	1290:1316	H2O2-mediated cross-linking	1290:1316	These results indicate that H2O2-mediated cross-linking and degradation of gelatin/hyaluronic acid composite hydrogel could be used to control the cell adhesion and cell-cycle progression.					
35269503	4	51	theme	cell-cycle	816:825	arg1	progressions					827:838	the exposure-time-dependent different cell-cycle progressions	778:838	the exposure-time-dependent different cell-cycle progressions on the hydrogels	778:855	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	3	52	theme	exposure	634:641	arg1	time					643:646	the exposure time	630:646	the exposure time	630:646	The stiffness of the hydrogel varied with the exposure time.					
35269503	1	53	theme	environment	230:240	arg1	interaction					246:256	interaction	246:256	interaction of cells with the adhering substrates	246:294	The cell cycle is known to be regulated by features such as the mechanical properties of the surrounding environment and interaction of cells with the adhering substrates.					
35269503	1	53	theme	environment	230:240	arg1	properties					200:209	the mechanical properties	185:209	the mechanical properties of the surrounding environment	185:240	The cell cycle is known to be regulated by features such as the mechanical properties of the surrounding environment and interaction of cells with the adhering substrates.					
35269503	4	54	theme	different	806:814	arg1	progressions					827:838	the exposure-time-dependent different cell-cycle progressions	778:838	the exposure-time-dependent different cell-cycle progressions on the hydrogels	778:855	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	1	55	with	interaction	246:256	arg1	substrates					285:294	the adhering substrates	272:294	the adhering substrates	272:294	The cell cycle is known to be regulated by features such as the mechanical properties of the surrounding environment and interaction of cells with the adhering substrates.					
35269503	4	56	theme	cervical	655:662	arg1	HeLa					678:681	HeLa	678:681	HeLa	678:681	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	4	56	theme	cervical	655:662	arg1	cells					671:675	Human cervical cancer cells	649:675	Human cervical cancer cells (HeLa)	649:682	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	5	57	theme	H2O2	995:998	arg1	exposure					1000:1007	the H2O2 exposure	991:1007	the H2O2 exposure	991:1007	Although HeLa/Fucci2 cells cultured on the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure showed a G2/M-phase arrest, NMuMG cells showed a G1-phase arrest.					
35269503	4	58	from	progressions	827:838	arg1	hydrogels					847:855	the hydrogels	843:855	the hydrogels	843:855	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	4	59	theme	mouse	688:692	arg1	cells					719:723	mouse mammary gland epithelial cells	688:723	mouse mammary gland epithelial cells (NMuMG)	688:731	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	4	59	theme	mouse	688:692	arg1	NMuMG					726:730	NMuMG	726:730	NMuMG	726:730	Human cervical cancer cells (HeLa) and mouse mammary gland epithelial cells (NMuMG) expressing cell-cycle reporter Fucci2 showed the exposure-time-dependent different cell-cycle progressions on the hydrogels.					
35269503	5	60	theme	soft	901:904	arg1	hydrogel					906:913	the soft hydrogel	897:913	the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure	897:1007	Although HeLa/Fucci2 cells cultured on the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure showed a G2/M-phase arrest, NMuMG cells showed a G1-phase arrest.					
35269503	5	61	theme	exposure	1000:1007	arg1	min					972:974	15 min	969:974	15 min	969:974	Although HeLa/Fucci2 cells cultured on the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure showed a G2/M-phase arrest, NMuMG cells showed a G1-phase arrest.					
35269503	5	61	theme	exposure	1000:1007	arg1	min					984:986	120 min	980:986	120 min of the H2O2 exposure	980:1007	Although HeLa/Fucci2 cells cultured on the soft hydrogel (Young's modulus: 0.20 and 0.40 kPa) obtained through 15 min and 120 min of the H2O2 exposure showed a G2/M-phase arrest, NMuMG cells showed a G1-phase arrest.					
35269503	2	62	theme	cells	379:383	arg1	progression					360:370	cell-cycle progression	349:370	cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air	349:585	Here, we investigated the possibility of regulating cell-cycle progression of the cells on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking and degradation of the polymers by varying the exposure time to H2O2 contained in the air.					
35746260	4	0	theme	energy	788:793	arg1	EDS					820:822	EDS	820:822	EDS	820:822	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	0	theme	energy	788:793	arg1	spectroscopy					806:817	energy dispersive spectroscopy	788:817	energy dispersive spectroscopy (EDS)	788:823	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	0	theme	energy	788:793	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy (TEM)	705:742	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	1	theme	impedance	941:949	arg1	EIS					965:967	EIS	965:967	EIS	965:967	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	1	theme	impedance	941:949	arg1	spectroscopy					951:962	electrochemical impedance spectroscopy	925:962	electrochemical impedance spectroscopy (EIS)	925:968	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	3	2	theme	fixed	622:626	arg1	chitosan					628:635	CNPs fixed chitosan	617:635	CNPs fixed chitosan	617:635	Then the CuNPs-CSF-CNPs-MWCNTs composite was facilely prepared by one-step co-electrodeposition of CuNPs and CNPs fixed chitosan residues on modified electrode.					
35746260	5	3	theme	composite	1095:1103	arg1	electrode					1114:1122	the composite modified electrode	1091:1122	the composite modified electrode	1091:1122	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	4	4	theme	linear	975:980	arg1	LSV					1001:1003	LSV	1001:1003	LSV	1001:1003	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	4	theme	linear	975:980	arg1	voltammetry					988:998	linear sweep voltammetry	975:998	linear sweep voltammetry (LSV)	975:1004	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	7	5	theme	leaf	1424:1427	arg1	samples					1429:1435	real leaf samples	1419:1435	real leaf samples with satisfactory recovery	1419:1462	In addition, the sensor could also be used for the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery.					
35746260	4	6	theme	electrochemical	925:939	arg1	EIS					965:967	EIS	965:967	EIS	965:967	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	6	theme	electrochemical	925:939	arg1	spectroscopy					951:962	electrochemical impedance spectroscopy	925:962	electrochemical impedance spectroscopy (EIS)	925:968	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	3	7	theme	chitosan	628:635	arg1	residues					637:644	CuNPs and CNPs fixed chitosan residues	607:644	CuNPs and CNPs fixed chitosan residues	607:644	Then the CuNPs-CSF-CNPs-MWCNTs composite was facilely prepared by one-step co-electrodeposition of CuNPs and CNPs fixed chitosan residues on modified electrode.					
35746260	0	8	theme	Indole-3-Acetic	151:165	arg1	Acid					167:170	Indole-3-Acetic Acid	151:170	Indole-3-Acetic Acid	151:170	One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid.					
35746260	5	9	theme	linear	1203:1208	arg1	relationship					1210:1221	a good linear relationship	1196:1221	a good linear relationship with SA	1196:1229	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	5	10	theme	linear	1135:1140	arg1	relationship					1142:1153	a good linear relationship	1128:1153	a good linear relationship with IAA in the range of 0.01-50 μM	1128:1189	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	4	11	theme	electron	718:725	arg1	microscope					687:696	Scanning electron microscope	669:696	Scanning electron microscope (SEM)	669:702	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	11	theme	electron	718:725	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy (TEM)	705:742	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	11	theme	electron	718:725	arg1	spectroscopy					806:817	energy dispersive spectroscopy	788:817	energy dispersive spectroscopy (EDS)	788:823	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	11	theme	electron	718:725	arg1	TEM					739:741	TEM	739:741	TEM	739:741	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	11	theme	electron	718:725	arg1	diffraction					832:842	X-ray diffraction	826:842	X-ray diffraction (XRD)	826:848	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	11	theme	electron	718:725	arg1	diffraction					768:778	selected area electron diffraction	745:778	selected area electron diffraction (SAED)	745:785	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	7	12	theme	IAA	1405:1407	arg1	detection					1392:1400	the simultaneous detection	1375:1400	the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery	1375:1462	In addition, the sensor could also be used for the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery.					
35746260	7	13	with	samples	1429:1435	arg1	recovery					1455:1462	satisfactory recovery	1442:1462	satisfactory recovery	1442:1462	In addition, the sensor could also be used for the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery.					
35746260	1	14	theme	novel	312:316	arg1	composite					427:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	7	15	theme	real	1419:1422	arg1	samples					1429:1435	real leaf samples	1419:1435	real leaf samples with satisfactory recovery	1419:1462	In addition, the sensor could also be used for the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery.					
35746260	5	16	from	relationship	1210:1221	arg1	range					1171:1175	the range	1167:1175	the range of 0.01-50 μM	1167:1189	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	7	17	from	detection	1392:1400	arg1	samples					1429:1435	real leaf samples	1419:1435	real leaf samples with satisfactory recovery	1419:1462	In addition, the sensor could also be used for the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery.					
35746260	3	18	theme	modified	649:656	arg1	electrode					658:666	modified electrode	649:666	modified electrode	649:666	Then the CuNPs-CSF-CNPs-MWCNTs composite was facilely prepared by one-step co-electrodeposition of CuNPs and CNPs fixed chitosan residues on modified electrode.					
35746260	3	19	theme	CuNPs-CSF-CNPs-MWCNTs	517:537	arg1	composite					539:547	Then the CuNPs-CSF-CNPs-MWCNTs composite	508:547	Then the CuNPs-CSF-CNPs-MWCNTs composite	508:547	Then the CuNPs-CSF-CNPs-MWCNTs composite was facilely prepared by one-step co-electrodeposition of CuNPs and CNPs fixed chitosan residues on modified electrode.					
35746260	1	20	theme	phytohormones	236:248	arg1	electroanalysis					217:231	A sensitive simultaneous electroanalysis	192:231	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	192:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	0	21	theme	Acid	167:170	arg1	Electroanalysis					132:146	Electroanalysis	132:146	Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid	132:189	One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid.					
35746260	7	22	theme	simultaneous	1379:1390	arg1	detection					1392:1400	the simultaneous detection	1375:1400	the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery	1375:1462	In addition, the sensor could also be used for the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery.					
35746260	5	23	with	relationship	1210:1221	arg1	IAA					1160:1162	IAA	1160:1162	IAA	1160:1162	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	5	23	with	relationship	1210:1221	arg1	SA					1228:1229	SA	1228:1229	SA	1228:1229	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	1	24	theme	indole-3-acetic	250:264	arg1	acid					266:269	indole-3-acetic acid	250:269	indole-3-acetic acid (IAA)	250:275	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	1	24	theme	indole-3-acetic	250:264	arg1	phytohormones					236:248	phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA)	236:299	phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	236:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	1	24	theme	indole-3-acetic	250:264	arg1	IAA					272:274	IAA	272:274	IAA	272:274	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	5	25	theme	optimal	1071:1077	arg1	conditions					1079:1088	optimal conditions	1071:1088	optimal conditions	1071:1088	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	7	26	theme	SA	1413:1414	arg1	detection					1392:1400	the simultaneous detection	1375:1400	the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery	1375:1462	In addition, the sensor could also be used for the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery.					
35746260	6	27	theme	detection	1260:1268	arg1	limits					1270:1275	The detection limits	1256:1275	The detection limits	1256:1275	The detection limits were 0.0086 μM and 0.7 μM (S/N = 3), respectively.					
35746260	6	27	theme	detection	1260:1268	arg1	μM					1289:1290	0.0086 μM	1282:1290	0.0086 μM	1282:1290	The detection limits were 0.0086 μM and 0.7 μM (S/N = 3), respectively.					
35746260	4	28	theme	microscope	687:696	arg1	Fourier					851:857	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier	669:857	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV)	669:1004	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	29	dep	Fourier	851:857	arg1	transform					859:867	transform	859:867	transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV)	859:1004	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	2	30	theme	hydrothermal	473:484	arg1	reaction					486:493	hydrothermal reaction	473:493	hydrothermal reaction of chitosan	473:505	CNPs were prepared by hydrothermal reaction of chitosan.					
35746260	0	31	theme	One-Step	0:7	arg1	Co-Electrodeposition					9:28	One-Step Co-Electrodeposition	0:28	One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon	0:106	One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid.					
35746260	1	32	theme	copper	318:323	arg1	composite					427:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	7	33	used	used	1366:1369	arg2	sensor					1345:1350	the sensor	1341:1350	the sensor	1341:1350	In addition, the sensor could also be used for the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery.					
35746260	4	34	theme	X-ray	826:830	arg1	XRD					845:847	XRD	845:847	XRD	845:847	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	34	theme	X-ray	826:830	arg1	diffraction					832:842	X-ray diffraction	826:842	X-ray diffraction (XRD)	826:848	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	34	theme	X-ray	826:830	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy (TEM)	705:742	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	5	35	from	relationship	1142:1153	arg1	range					1171:1175	the range	1167:1175	the range of 0.01-50 μM	1167:1189	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	0	36	theme	Copper	33:38	arg1	Carbon					101:106	Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon	33:106	Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon	33:106	One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid.					
35746260	1	37	theme	nanoparticles-chitosan	325:346	arg1	composite					427:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	0	38	theme	Acid	186:189	arg1	Electroanalysis					132:146	Electroanalysis	132:146	Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid	132:189	One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid.					
35746260	3	39	theme	one-step	574:581	arg1	co-electrodeposition					583:602	one-step co-electrodeposition	574:602	one-step co-electrodeposition of CuNPs and CNPs fixed chitosan residues on modified electrode	574:666	Then the CuNPs-CSF-CNPs-MWCNTs composite was facilely prepared by one-step co-electrodeposition of CuNPs and CNPs fixed chitosan residues on modified electrode.					
35746260	5	40	contain	had	1124:1126	arg2	relationship					1142:1153	a good linear relationship	1128:1153	a good linear relationship with IAA in the range of 0.01-50 μM	1128:1189	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	5	40	contain	had	1124:1126	arg2	relationship					1210:1221	a good linear relationship	1196:1221	a good linear relationship with SA	1196:1229	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	5	40	contain	had	1124:1126	arg1	electrode					1114:1122	the composite modified electrode	1091:1122	the composite modified electrode	1091:1122	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	1	41	theme	film-carbon	348:358	arg1	composite					427:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	0	42	theme	Salicylic	176:184	arg1	Acid					186:189	Salicylic Acid	176:189	Salicylic Acid	176:189	One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid.					
35746260	4	43	theme	dispersive	795:804	arg1	EDS					820:822	EDS	820:822	EDS	820:822	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	43	theme	dispersive	795:804	arg1	spectroscopy					806:817	energy dispersive spectroscopy	788:817	energy dispersive spectroscopy (EDS)	788:823	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	43	theme	dispersive	795:804	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy (TEM)	705:742	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	3	44	theme	residues	637:644	arg1	co-electrodeposition					583:602	one-step co-electrodeposition	574:602	one-step co-electrodeposition of CuNPs and CNPs fixed chitosan residues on modified electrode	574:666	Then the CuNPs-CSF-CNPs-MWCNTs composite was facilely prepared by one-step co-electrodeposition of CuNPs and CNPs fixed chitosan residues on modified electrode.					
35746260	0	45	theme	Film-Carbon	63:73	arg1	Carbon					101:106	Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon	33:106	Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon	33:106	One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid.					
35746260	1	46	theme	nanoparticles-multiwalled	360:384	arg1	composite					427:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	4	47	theme	area	754:757	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy (TEM)	705:742	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	47	theme	area	754:757	arg1	diffraction					768:778	selected area electron diffraction	745:778	selected area electron diffraction (SAED)	745:785	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	7	48	theme	satisfactory	1442:1453	arg1	recovery					1455:1462	satisfactory recovery	1442:1462	satisfactory recovery	1442:1462	In addition, the sensor could also be used for the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery.					
35746260	4	49	theme	electron	759:766	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy (TEM)	705:742	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	49	theme	electron	759:766	arg1	diffraction					768:778	selected area electron diffraction	745:778	selected area electron diffraction (SAED)	745:785	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	0	50	theme	Nanoparticles-Chitosan	40:61	arg1	Carbon					101:106	Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon	33:106	Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon	33:106	One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid.					
35746260	1	51	theme	carbon	386:391	arg1	composite					427:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	5	52	theme	good	1130:1133	arg1	relationship					1142:1153	a good linear relationship	1128:1153	a good linear relationship with IAA in the range of 0.01-50 μM	1128:1189	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	5	53	theme	μM	1252:1253	arg1	range					1238:1242	the range	1234:1242	the range of 4-30 μM	1234:1253	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	1	54	theme	salicylic	281:289	arg1	SA					297:298	SA	297:298	SA	297:298	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	1	54	theme	salicylic	281:289	arg1	acid					291:294	salicylic acid	281:294	salicylic acid (SA)	281:299	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	1	54	theme	salicylic	281:289	arg1	phytohormones					236:248	phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA)	236:299	phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	236:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	0	55	theme	Carbon	101:106	arg1	Co-Electrodeposition					9:28	One-Step Co-Electrodeposition	0:28	One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon	0:106	One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid.					
35746260	1	56	theme	nanotubes	393:401	arg1	composite					427:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	6	57	theme	=	1308:1308	arg1	S/N					1304:1306	S/N = 3	1304:1310	S/N = 3	1304:1310	The detection limits were 0.0086 μM and 0.7 μM (S/N = 3), respectively.					
35746260	6	57	theme	=	1308:1308	arg1	μM					1300:1301	0.7 μM	1296:1301	0.7 μM (S/N = 3)	1296:1311	The detection limits were 0.0086 μM and 0.7 μM (S/N = 3), respectively.					
35746260	4	58	theme	electron	678:685	arg1	microscope					687:696	Scanning electron microscope	669:696	Scanning electron microscope (SEM)	669:702	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	58	theme	electron	678:685	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy (TEM)	705:742	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	58	theme	electron	678:685	arg1	SEM					699:701	SEM	699:701	SEM	699:701	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	1	59	dep	phytohormones	236:248	arg1	acid					266:269	indole-3-acetic acid	250:269	indole-3-acetic acid (IAA)	250:275	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	1	59	dep	phytohormones	236:248	arg1	SA					297:298	SA	297:298	SA	297:298	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	1	59	dep	phytohormones	236:248	arg1	acid					291:294	salicylic acid	281:294	salicylic acid (SA)	281:299	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	1	59	dep	phytohormones	236:248	arg1	phytohormones					236:248	phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA)	236:299	phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	236:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	1	59	dep	phytohormones	236:248	arg1	IAA					272:274	IAA	272:274	IAA	272:274	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	0	60	theme	Nanoparticles-Multiwalled	75:99	arg1	Carbon					101:106	Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon	33:106	Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon	33:106	One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid.					
35746260	4	61	theme	Scanning	669:676	arg1	microscope					687:696	Scanning electron microscope	669:696	Scanning electron microscope (SEM)	669:702	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	61	theme	Scanning	669:676	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy (TEM)	705:742	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	61	theme	Scanning	669:676	arg1	SEM					699:701	SEM	699:701	SEM	699:701	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	62	theme	sweep	982:986	arg1	LSV					1001:1003	LSV	1001:1003	LSV	1001:1003	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	62	theme	sweep	982:986	arg1	voltammetry					988:998	linear sweep voltammetry	975:998	linear sweep voltammetry (LSV)	975:1004	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	63	theme	cyclic	900:905	arg1	CV					920:921	CV	920:921	CV	920:921	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	63	theme	cyclic	900:905	arg1	voltammetry					907:917	cyclic voltammetry	900:917	cyclic voltammetry (CV)	900:922	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	5	64	theme	μM	1188:1189	arg1	range					1171:1175	the range	1167:1175	the range of 0.01-50 μM	1167:1189	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	3	65	theme	CNPs	617:620	arg1	chitosan					628:635	CNPs fixed chitosan	617:635	CNPs fixed chitosan	617:635	Then the CuNPs-CSF-CNPs-MWCNTs composite was facilely prepared by one-step co-electrodeposition of CuNPs and CNPs fixed chitosan residues on modified electrode.					
35746260	4	66	theme	composite	1054:1062	arg1	properties					1036:1045	the properties	1032:1045	the properties of the composite	1032:1062	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	1	67	theme	CuNPs-CSF-CNPs-MWCNTs	404:424	arg1	composite					427:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	310:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	4	68	used	used	1011:1014	arg2	Fourier					851:857	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier	669:857	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV)	669:1004	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	3	69	from	co-electrodeposition	583:602	arg1	electrode					658:666	modified electrode	649:666	modified electrode	649:666	Then the CuNPs-CSF-CNPs-MWCNTs composite was facilely prepared by one-step co-electrodeposition of CuNPs and CNPs fixed chitosan residues on modified electrode.					
35746260	5	70	theme	modified	1105:1112	arg1	electrode					1114:1122	the composite modified electrode	1091:1122	the composite modified electrode	1091:1122	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	4	71	theme	infrared	869:876	arg1	FT-IR					892:896	FT-IR	892:896	FT-IR	892:896	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	71	theme	infrared	869:876	arg1	spectroscopy					878:889	infrared spectroscopy	869:889	infrared spectroscopy (FT-IR)	869:897	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	3	72	theme	CuNPs	607:611	arg1	residues					637:644	CuNPs and CNPs fixed chitosan residues	607:644	CuNPs and CNPs fixed chitosan residues	607:644	Then the CuNPs-CSF-CNPs-MWCNTs composite was facilely prepared by one-step co-electrodeposition of CuNPs and CNPs fixed chitosan residues on modified electrode.					
35746260	5	73	with	relationship	1142:1153	arg1	IAA					1160:1162	IAA	1160:1162	IAA	1160:1162	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	5	73	with	relationship	1142:1153	arg1	SA					1228:1229	SA	1228:1229	SA	1228:1229	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	2	74	theme	chitosan	498:505	arg1	reaction					486:493	hydrothermal reaction	473:493	hydrothermal reaction of chitosan	473:505	CNPs were prepared by hydrothermal reaction of chitosan.					
35746260	4	75	theme	selected	745:752	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy (TEM)	705:742	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	75	theme	selected	745:752	arg1	diffraction					768:778	selected area electron diffraction	745:778	selected area electron diffraction (SAED)	745:785	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	1	76	theme	sensitive	194:202	arg1	electroanalysis					217:231	A sensitive simultaneous electroanalysis	192:231	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	192:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
35746260	5	77	theme	good	1198:1201	arg1	relationship					1210:1221	a good linear relationship	1196:1221	a good linear relationship with SA	1196:1229	Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01-50 μM, and a good linear relationship with SA in the range of 4-30 μM.					
35746260	4	78	theme	transmission	705:716	arg1	microscope					687:696	Scanning electron microscope	669:696	Scanning electron microscope (SEM)	669:702	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	78	theme	transmission	705:716	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy (TEM)	705:742	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	78	theme	transmission	705:716	arg1	spectroscopy					806:817	energy dispersive spectroscopy	788:817	energy dispersive spectroscopy (EDS)	788:823	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	78	theme	transmission	705:716	arg1	TEM					739:741	TEM	739:741	TEM	739:741	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	78	theme	transmission	705:716	arg1	diffraction					832:842	X-ray diffraction	826:842	X-ray diffraction (XRD)	826:848	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	78	theme	transmission	705:716	arg1	diffraction					768:778	selected area electron diffraction	745:778	selected area electron diffraction (SAED)	745:785	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	4	79	dep	diffraction	768:778	arg1	SAED					781:784	SAED	781:784	SAED	781:784	Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite.					
35746260	1	80	theme	simultaneous	204:215	arg1	electroanalysis					217:231	A sensitive simultaneous electroanalysis	192:231	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite	192:435	A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported.					
36283782	5	0	from	pM	1022:1023	arg1	range					1007:1011	a linear range	998:1011	a linear range from 0.1 pM to 1.0 μM	998:1033	This proposed photoelectrochemical sensing system displayed selective detection of Hg2+ with a linear range from 0.1 pM to 1.0 μM with a detection limit of 0.067 pM.					
36283782	6	1	theme	photoelectrochemical	1230:1249	arg1	systems					1259:1265	photoelectrochemical sensing systems	1230:1265	photoelectrochemical sensing systems	1230:1265	The utilization of semiconductor quantum dots as light-harvesting components and the MOFs-like composite as sensitizers broadens the possible design ideas for photoelectrochemical sensing systems.					
36283782	4	2	theme	probe	679:683	arg1	bent					728:731	bent	728:731	bent	728:731	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	4	2	theme	probe	679:683	arg1	DNA					685:687	the immobilized thymidine-rich probe DNA	648:687	the immobilized thymidine-rich probe DNA on the MOFs-like composite surface	648:722	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	0	3	theme	MOFs-like	74:82	arg1	structures					84:93	in situ generated MOFs-like structures	56:93	in situ generated MOFs-like structures	56:93	Photoelectrochemical sensor for detection Hg2+ based on in situ generated MOFs-like structures.					
36283782	1	4	theme	great	140:144	arg1	significance					146:157	great significance	140:157	great significance	140:157	Trace analysis of mercury ions (Hg2+) is of great significance to human health and environmental protection.					
36283782	5	5	theme	Hg2+	988:991	arg1	detection					975:983	selective detection	965:983	selective detection of Hg2+ with a linear range from 0.1 pM to 1.0 μM	965:1033	This proposed photoelectrochemical sensing system displayed selective detection of Hg2+ with a linear range from 0.1 pM to 1.0 μM with a detection limit of 0.067 pM.					
36283782	5	6	theme	sensing	940:946	arg1	system					948:953	This proposed photoelectrochemical sensing system	905:953	This proposed photoelectrochemical sensing system	905:953	This proposed photoelectrochemical sensing system displayed selective detection of Hg2+ with a linear range from 0.1 pM to 1.0 μM with a detection limit of 0.067 pM.					
36283782	5	7	theme	proposed	910:917	arg1	system					948:953	This proposed photoelectrochemical sensing system	905:953	This proposed photoelectrochemical sensing system	905:953	This proposed photoelectrochemical sensing system displayed selective detection of Hg2+ with a linear range from 0.1 pM to 1.0 μM with a detection limit of 0.067 pM.					
36283782	4	8	from	hindrance	819:827	arg1	surface					846:852	the electrode surface	832:852	the electrode surface	832:852	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	4	9	theme	electrode	836:844	arg1	surface					846:852	the electrode surface	832:852	the electrode surface	832:852	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	6	10	theme	MOFs-like	1156:1164	arg1	composite					1166:1174	the MOFs-like composite	1152:1174	the MOFs-like composite	1152:1174	The utilization of semiconductor quantum dots as light-harvesting components and the MOFs-like composite as sensitizers broadens the possible design ideas for photoelectrochemical sensing systems.					
36283782	3	11	theme	modified	469:476	arg1	HMA					488:490	HMA	488:490	HMA	488:490	The MOFs-like composite in situ formed by hydrophobically modified alginate (HMA) with europium ion (Eu3+) not only offered a friendly platform for bioconjugation but also resulted in enhancing sensor photocurrent response.					
36283782	3	11	theme	modified	469:476	arg1	alginate					478:485	hydrophobically modified alginate	453:485	hydrophobically modified alginate (HMA) with europium ion (Eu3+)	453:516	The MOFs-like composite in situ formed by hydrophobically modified alginate (HMA) with europium ion (Eu3+) not only offered a friendly platform for bioconjugation but also resulted in enhancing sensor photocurrent response.					
36283782	4	12	theme	enlarged	803:810	arg1	hindrance					819:827	enlarged steric hindrance	803:827	enlarged steric hindrance on the electrode surface	803:852	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	2	13	theme	photoactive	378:388	arg1	materials					400:408	photoactive substrate materials	378:408	photoactive substrate materials	378:408	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
36283782	3	14	theme	europium	498:505	arg1	Eu3+					512:515	Eu3+	512:515	Eu3+	512:515	The MOFs-like composite in situ formed by hydrophobically modified alginate (HMA) with europium ion (Eu3+) not only offered a friendly platform for bioconjugation but also resulted in enhancing sensor photocurrent response.					
36283782	3	14	theme	europium	498:505	arg1	ion					507:509	europium ion	498:509	europium ion (Eu3+)	498:516	The MOFs-like composite in situ formed by hydrophobically modified alginate (HMA) with europium ion (Eu3+) not only offered a friendly platform for bioconjugation but also resulted in enhancing sensor photocurrent response.					
36283782	6	15	theme	semiconductor	1090:1102	arg1	dots					1112:1115	semiconductor quantum dots	1090:1115	semiconductor quantum dots	1090:1115	The utilization of semiconductor quantum dots as light-harvesting components and the MOFs-like composite as sensitizers broadens the possible design ideas for photoelectrochemical sensing systems.					
36283782	2	16	theme	PEC	249:251	arg1	biosensor					254:262	a novel photoelectrochemical (PEC) biosensor	219:262	a novel photoelectrochemical (PEC) biosensor	219:262	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
36283782	3	17	theme	MOFs-like	415:423	arg1	composite					425:433	The MOFs-like composite	411:433	The MOFs-like composite in situ formed by hydrophobically modified alginate (HMA) with europium ion (Eu3+)	411:516	The MOFs-like composite in situ formed by hydrophobically modified alginate (HMA) with europium ion (Eu3+) not only offered a friendly platform for bioconjugation but also resulted in enhancing sensor photocurrent response.					
36283782	6	18	theme	sensing	1251:1257	arg1	systems					1259:1265	photoelectrochemical sensing systems	1230:1265	photoelectrochemical sensing systems	1230:1265	The utilization of semiconductor quantum dots as light-harvesting components and the MOFs-like composite as sensitizers broadens the possible design ideas for photoelectrochemical sensing systems.					
36283782	2	19	theme	ultrasensitive	286:299	arg1	detection					301:309	the ultrasensitive detection	282:309	the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials	282:408	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
36283782	5	20	theme	pM	1067:1068	arg1	limit					1052:1056	a detection limit	1040:1056	a detection limit of 0.067 pM	1040:1068	This proposed photoelectrochemical sensing system displayed selective detection of Hg2+ with a linear range from 0.1 pM to 1.0 μM with a detection limit of 0.067 pM.					
36283782	1	21	theme	human	162:166	arg1	health					168:173	human health	162:173	human health	162:173	Trace analysis of mercury ions (Hg2+) is of great significance to human health and environmental protection.					
36283782	0	22	theme	Photoelectrochemical	0:19	arg1	sensor					21:26	Photoelectrochemical sensor	0:26	Photoelectrochemical sensor for detection Hg2+	0:45	Photoelectrochemical sensor for detection Hg2+ based on in situ generated MOFs-like structures.					
36283782	3	23	with	alginate	478:485	arg1	Eu3+					512:515	Eu3+	512:515	Eu3+	512:515	The MOFs-like composite in situ formed by hydrophobically modified alginate (HMA) with europium ion (Eu3+) not only offered a friendly platform for bioconjugation but also resulted in enhancing sensor photocurrent response.					
36283782	3	23	with	alginate	478:485	arg1	ion					507:509	europium ion	498:509	europium ion (Eu3+)	498:516	The MOFs-like composite in situ formed by hydrophobically modified alginate (HMA) with europium ion (Eu3+) not only offered a friendly platform for bioconjugation but also resulted in enhancing sensor photocurrent response.					
36283782	1	24	theme	Trace	96:100	arg1	analysis					102:109	Trace analysis	96:109	Trace analysis of mercury ions (Hg2+)	96:132	Trace analysis of mercury ions (Hg2+) is of great significance to human health and environmental protection.					
36283782	2	25	theme	quantum	356:362	arg1	QDs					370:372	QDs	370:372	QDs	370:372	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
36283782	2	25	theme	quantum	356:362	arg1	dots					364:367	the MOFs-like composite/CdS quantum dots	328:367	the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials	328:408	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
36283782	0	26	theme	detection	32:40	arg1	Hg2+					42:45	detection Hg2+	32:45	detection Hg2+	32:45	Photoelectrochemical sensor for detection Hg2+ based on in situ generated MOFs-like structures.					
36283782	4	27	theme	PEC	891:893	arg1	response					895:902	the proposed biosensor PEC response	868:902	the proposed biosensor PEC response	868:902	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	6	28	theme	possible	1204:1211	arg1	ideas					1220:1224	the possible design ideas	1200:1224	the possible design ideas for photoelectrochemical sensing systems	1200:1265	The utilization of semiconductor quantum dots as light-harvesting components and the MOFs-like composite as sensitizers broadens the possible design ideas for photoelectrochemical sensing systems.					
36283782	5	29	theme	photoelectrochemical	919:938	arg1	system					948:953	This proposed photoelectrochemical sensing system	905:953	This proposed photoelectrochemical sensing system	905:953	This proposed photoelectrochemical sensing system displayed selective detection of Hg2+ with a linear range from 0.1 pM to 1.0 μM with a detection limit of 0.067 pM.					
36283782	5	30	theme	linear	1000:1005	arg1	range					1007:1011	a linear range	998:1011	a linear range from 0.1 pM to 1.0 μM	998:1033	This proposed photoelectrochemical sensing system displayed selective detection of Hg2+ with a linear range from 0.1 pM to 1.0 μM with a detection limit of 0.067 pM.					
36283782	2	31	theme	substrate	390:398	arg1	materials					400:408	photoactive substrate materials	378:408	photoactive substrate materials	378:408	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
36283782	4	32	theme	composite	706:714	arg1	surface					716:722	the MOFs-like composite surface	692:722	the MOFs-like composite surface	692:722	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	4	33	theme	biosensor	881:889	arg1	response					895:902	the proposed biosensor PEC response	868:902	the proposed biosensor PEC response	868:902	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	3	34	theme	sensor	605:610	arg1	response					625:632	sensor photocurrent response	605:632	sensor photocurrent response	605:632	The MOFs-like composite in situ formed by hydrophobically modified alginate (HMA) with europium ion (Eu3+) not only offered a friendly platform for bioconjugation but also resulted in enhancing sensor photocurrent response.					
36283782	4	35	theme	thymidine-rich	664:677	arg1	bent					728:731	bent	728:731	bent	728:731	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	4	35	theme	thymidine-rich	664:677	arg1	DNA					685:687	the immobilized thymidine-rich probe DNA	648:687	the immobilized thymidine-rich probe DNA on the MOFs-like composite surface	648:722	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	1	36	theme	environmental	179:191	arg1	protection					193:202	environmental protection	179:202	environmental protection	179:202	Trace analysis of mercury ions (Hg2+) is of great significance to human health and environmental protection.					
36283782	2	37	theme	Hg2+	314:317	arg1	detection					301:309	the ultrasensitive detection	282:309	the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials	282:408	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
36283782	3	38	theme	photocurrent	612:623	arg1	response					625:632	sensor photocurrent response	605:632	sensor photocurrent response	605:632	The MOFs-like composite in situ formed by hydrophobically modified alginate (HMA) with europium ion (Eu3+) not only offered a friendly platform for bioconjugation but also resulted in enhancing sensor photocurrent response.					
36283782	4	39	theme	Hg2+	784:787	arg1	presence					772:779	the presence	768:779	the presence of Hg2+	768:787	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	4	40	theme	immobilized	652:662	arg1	bent					728:731	bent	728:731	bent	728:731	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	4	40	theme	immobilized	652:662	arg1	DNA					685:687	the immobilized thymidine-rich probe DNA	648:687	the immobilized thymidine-rich probe DNA on the MOFs-like composite surface	648:722	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	5	41	with	detection	975:983	arg1	range					1007:1011	a linear range	998:1011	a linear range from 0.1 pM to 1.0 μM	998:1033	This proposed photoelectrochemical sensing system displayed selective detection of Hg2+ with a linear range from 0.1 pM to 1.0 μM with a detection limit of 0.067 pM.					
36283782	6	42	theme	design	1213:1218	arg1	ideas					1220:1224	the possible design ideas	1200:1224	the possible design ideas for photoelectrochemical sensing systems	1200:1265	The utilization of semiconductor quantum dots as light-harvesting components and the MOFs-like composite as sensitizers broadens the possible design ideas for photoelectrochemical sensing systems.					
36283782	4	43	theme	steric	812:817	arg1	hindrance					819:827	enlarged steric hindrance	803:827	enlarged steric hindrance on the electrode surface	803:852	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	1	44	theme	mercury	114:120	arg1	Hg2+					128:131	Hg2+	128:131	Hg2+	128:131	Trace analysis of mercury ions (Hg2+) is of great significance to human health and environmental protection.					
36283782	1	44	theme	mercury	114:120	arg1	ions					122:125	mercury ions	114:125	mercury ions (Hg2+)	114:132	Trace analysis of mercury ions (Hg2+) is of great significance to human health and environmental protection.					
36283782	3	45	theme	friendly	537:544	arg1	platform					546:553	a friendly platform	535:553	a friendly platform for bioconjugation	535:572	The MOFs-like composite in situ formed by hydrophobically modified alginate (HMA) with europium ion (Eu3+) not only offered a friendly platform for bioconjugation but also resulted in enhancing sensor photocurrent response.					
36283782	1	46	theme	ions	122:125	arg1	analysis					102:109	Trace analysis	96:109	Trace analysis of mercury ions (Hg2+)	96:132	Trace analysis of mercury ions (Hg2+) is of great significance to human health and environmental protection.					
36283782	4	47	theme	proposed	872:879	arg1	response					895:902	the proposed biosensor PEC response	868:902	the proposed biosensor PEC response	868:902	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	4	48	theme	MOFs-like	696:704	arg1	surface					716:722	the MOFs-like composite surface	692:722	the MOFs-like composite surface	692:722	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	4	49	theme	T-Hg2+-T	746:753	arg1	structure					755:763	a T-Hg2+-T structure	744:763	a T-Hg2+-T structure	744:763	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	2	50	theme	photoelectrochemical	227:246	arg1	biosensor					254:262	a novel photoelectrochemical (PEC) biosensor	219:262	a novel photoelectrochemical (PEC) biosensor	219:262	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
36283782	5	51	theme	detection	1042:1050	arg1	limit					1052:1056	a detection limit	1040:1056	a detection limit of 0.067 pM	1040:1068	This proposed photoelectrochemical sensing system displayed selective detection of Hg2+ with a linear range from 0.1 pM to 1.0 μM with a detection limit of 0.067 pM.					
36283782	6	52	theme	quantum	1104:1110	arg1	dots					1112:1115	semiconductor quantum dots	1090:1115	semiconductor quantum dots	1090:1115	The utilization of semiconductor quantum dots as light-harvesting components and the MOFs-like composite as sensitizers broadens the possible design ideas for photoelectrochemical sensing systems.					
36283782	0	53	theme	generated	64:72	arg1	structures					84:93	in situ generated MOFs-like structures	56:93	in situ generated MOFs-like structures	56:93	Photoelectrochemical sensor for detection Hg2+ based on in situ generated MOFs-like structures.					
36283782	5	54	theme	selective	965:973	arg1	detection					975:983	selective detection	965:983	selective detection of Hg2+ with a linear range from 0.1 pM to 1.0 μM	965:1033	This proposed photoelectrochemical sensing system displayed selective detection of Hg2+ with a linear range from 0.1 pM to 1.0 μM with a detection limit of 0.067 pM.					
36283782	2	55	theme	novel	221:225	arg1	biosensor					254:262	a novel photoelectrochemical (PEC) biosensor	219:262	a novel photoelectrochemical (PEC) biosensor	219:262	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
36283782	2	56	theme	composite/CdS	342:354	arg1	QDs					370:372	QDs	370:372	QDs	370:372	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
36283782	2	56	theme	composite/CdS	342:354	arg1	dots					364:367	the MOFs-like composite/CdS quantum dots	328:367	the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials	328:408	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
36283782	4	57	from	DNA	685:687	arg1	surface					716:722	the MOFs-like composite surface	692:722	the MOFs-like composite surface	692:722	Furthermore, the immobilized thymidine-rich probe DNA on the MOFs-like composite surface was bent to produce a T-Hg2+-T structure in the presence of Hg2+, resulting in enlarged steric hindrance on the electrode surface and decreased the proposed biosensor PEC response.					
36283782	6	58	theme	light-harvesting	1120:1135	arg1	components					1137:1146	light-harvesting components	1120:1146	light-harvesting components	1120:1146	The utilization of semiconductor quantum dots as light-harvesting components and the MOFs-like composite as sensitizers broadens the possible design ideas for photoelectrochemical sensing systems.					
36283782	6	59	theme	dots	1112:1115	arg1	utilization					1075:1085	The utilization	1071:1085	The utilization of semiconductor quantum dots as light-harvesting components and the MOFs-like composite as sensitizers	1071:1189	The utilization of semiconductor quantum dots as light-harvesting components and the MOFs-like composite as sensitizers broadens the possible design ideas for photoelectrochemical sensing systems.					
36283782	2	60	theme	MOFs-like	332:340	arg1	QDs					370:372	QDs	370:372	QDs	370:372	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
36283782	2	60	theme	MOFs-like	332:340	arg1	dots					364:367	the MOFs-like composite/CdS quantum dots	328:367	the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials	328:408	In this work, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of Hg2+ based on the MOFs-like composite/CdS quantum dots (QDs) as photoactive substrate materials.					
37303253	0	0	theme	sap	72:74	arg1	movement					76:83	phloem sap movement	65:83	phloem sap movement	65:83	Revisiting yield in terms of phloem transport to grains suggests phloem sap movement might be homeostatic.					
37303253	4	1	theme	phloem	927:932	arg1	transport					936:944	phloem N transport	927:944	phloem N transport	927:944	Our results suggest that phloem sucrose transport rate follows the same relationship with phloem N transport regardless of irrigation conditions and cultivars, and seems to depend mostly on grain weight (i.e., mg per grain).					
37303253	3	2	from	consumption	639:649	arg1	cultivars					667:675	winter wheat cultivars	654:675	winter wheat cultivars grown across several sites with or without irrigation	654:729	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	4	3	dep	mg	1047:1048	arg1	i.e.					1041:1044	i.e.	1041:1044	i.e.	1041:1044	Our results suggest that phloem sucrose transport rate follows the same relationship with phloem N transport regardless of irrigation conditions and cultivars, and seems to depend mostly on grain weight (i.e., mg per grain).					
37303253	2	4	theme	carbon	329:334	arg1	allocation					336:345	carbon allocation	329:345	carbon allocation to grains	329:355	Although there is clear evidence that carbon allocation to grains effectively drives yield in cereals like wheat (as reflected by the harvest index), the influence of phloem transport rate and velocity is less clear.					
37303253	3	5	theme	water	820:824	arg1	transport					826:834	xylem water transport	814:834	xylem water transport	814:834	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	1	6	theme	yield	224:228	arg1	limitations					204:214	physiological limitations	190:214	physiological limitations of crop yield, along with photosynthetic activity or water use efficiency	190:288	Phloem sap transport, velocity and allocation have been proposed to play a role in physiological limitations of crop yield, along with photosynthetic activity or water use efficiency.					
37303253	0	7	theme	phloem	65:70	arg1	movement					76:83	phloem sap movement	65:83	phloem sap movement	65:83	Revisiting yield in terms of phloem transport to grains suggests phloem sap movement might be homeostatic.					
37303253	0	8	from	yield	11:15	arg1	terms					20:24	terms	20:24	terms of phloem transport to grains	20:54	Revisiting yield in terms of phloem transport to grains suggests phloem sap movement might be homeostatic.					
37303253	7	9	from	limitation	1526:1535	arg1	wheat					1550:1554	wheat	1550:1554	wheat	1550:1554	This suggests that phloem transport per se is not a limitation for yield in wheat but rather, is controlled to sustain grain filling.					
37303253	5	10	theme	proportionality	1167:1181	arg1	coefficient					1183:1193	its proportionality coefficient	1163:1193	its proportionality coefficient to xylem velocity change little with environmental conditions	1163:1255	Depending on the assumption made for phloem sap sucrose concentration, either phloem sap velocity or its proportionality coefficient to xylem velocity change little with environmental conditions.					
37303253	5	11	theme	sucrose	1110:1116	arg1	concentration					1118:1130	phloem sap sucrose concentration	1099:1130	phloem sap sucrose concentration	1099:1130	Depending on the assumption made for phloem sap sucrose concentration, either phloem sap velocity or its proportionality coefficient to xylem velocity change little with environmental conditions.					
37303253	6	12	theme	plant	1410:1414	arg1	parameters					1430:1439	other plant physiological parameters	1404:1439	other plant physiological parameters across cultivars and conditions	1404:1471	Taken as a whole, phloem transport from leaves to grains seems to be homeostatic within a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions.					
37303253	6	13	theme	phloem	1276:1281	arg1	transport					1283:1291	phloem transport	1276:1291	phloem transport from leaves to grains	1276:1313	Taken as a whole, phloem transport from leaves to grains seems to be homeostatic within a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions.					
37303253	2	14	theme	rate	475:478	arg1	influence					445:453	the influence	441:453	the influence of phloem transport rate and velocity	441:491	Although there is clear evidence that carbon allocation to grains effectively drives yield in cereals like wheat (as reflected by the harvest index), the influence of phloem transport rate and velocity is less clear.					
37303253	3	15	theme	wheat	661:665	arg1	cultivars					667:675	winter wheat cultivars	654:675	winter wheat cultivars grown across several sites with or without irrigation	654:729	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	6	16	with	range	1355:1359	arg1	parameters					1430:1439	other plant physiological parameters	1404:1439	other plant physiological parameters across cultivars and conditions	1404:1471	Taken as a whole, phloem transport from leaves to grains seems to be homeostatic within a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions.					
37303253	7	17	theme	grain	1593:1597	arg1	filling					1599:1605	grain filling	1593:1605	grain filling	1593:1605	This suggests that phloem transport per se is not a limitation for yield in wheat but rather, is controlled to sustain grain filling.					
37303253	2	18	theme	transport	465:473	arg1	rate					475:478	phloem transport rate	458:478	phloem transport rate	458:478	Although there is clear evidence that carbon allocation to grains effectively drives yield in cereals like wheat (as reflected by the harvest index), the influence of phloem transport rate and velocity is less clear.					
37303253	7	19	dep	limitation	1526:1535	arg1	controlled					1571:1580	controlled	1571:1580	is controlled to sustain grain filling	1568:1605	This suggests that phloem transport per se is not a limitation for yield in wheat but rather, is controlled to sustain grain filling.					
37303253	4	20	theme	grain	1027:1031	arg1	weight					1033:1038	grain weight	1027:1038	grain weight (i.e., mg per grain)	1027:1059	Our results suggest that phloem sucrose transport rate follows the same relationship with phloem N transport regardless of irrigation conditions and cultivars, and seems to depend mostly on grain weight (i.e., mg per grain).					
37303253	2	21	theme	phloem	458:463	arg1	rate					475:478	phloem transport rate	458:478	phloem transport rate	458:478	Although there is clear evidence that carbon allocation to grains effectively drives yield in cereals like wheat (as reflected by the harvest index), the influence of phloem transport rate and velocity is less clear.					
37303253	3	22	from	content	621:627	arg1	cultivars					667:675	winter wheat cultivars	654:675	winter wheat cultivars grown across several sites with or without irrigation	654:729	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	7	23	theme	phloem	1493:1498	arg1	transport					1500:1508	phloem transport	1493:1508	phloem transport	1493:1508	This suggests that phloem transport per se is not a limitation for yield in wheat but rather, is controlled to sustain grain filling.					
37303253	7	23	theme	phloem	1493:1498	arg1	limitation					1526:1535	a limitation	1524:1535	a limitation for yield in wheat	1524:1554	This suggests that phloem transport per se is not a limitation for yield in wheat but rather, is controlled to sustain grain filling.					
37303253	4	24	theme	irrigation	960:969	arg1	conditions					971:980	irrigation conditions	960:980	irrigation conditions	960:980	Our results suggest that phloem sucrose transport rate follows the same relationship with phloem N transport regardless of irrigation conditions and cultivars, and seems to depend mostly on grain weight (i.e., mg per grain).					
37303253	3	25	theme	winter	654:659	arg1	cultivars					667:675	winter wheat cultivars	654:675	winter wheat cultivars grown across several sites with or without irrigation	654:729	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	3	26	theme	several	690:696	arg1	sites					698:702	several sites	690:702	several sites with or without irrigation	690:729	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	2	27	dep	is	306:307	arg1	reflected					408:416	reflected	408:416	reflected by the harvest index	408:437	Although there is clear evidence that carbon allocation to grains effectively drives yield in cereals like wheat (as reflected by the harvest index), the influence of phloem transport rate and velocity is less clear.					
37303253	3	28	from	yield	564:568	arg1	cultivars					667:675	winter wheat cultivars	654:675	winter wheat cultivars grown across several sites with or without irrigation	654:729	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	0	29	theme	Revisiting	0:9	arg1	yield					11:15	Revisiting yield	0:15	Revisiting yield in terms of phloem transport to grains	0:54	Revisiting yield in terms of phloem transport to grains suggests phloem sap movement might be homeostatic.					
37303253	1	30	theme	photosynthetic	242:255	arg1	activity					257:264	photosynthetic activity	242:264	photosynthetic activity	242:264	Phloem sap transport, velocity and allocation have been proposed to play a role in physiological limitations of crop yield, along with photosynthetic activity or water use efficiency.					
37303253	2	31	dep	reflected	408:416	arg1	as					405:406	as	405:406	as	405:406	Although there is clear evidence that carbon allocation to grains effectively drives yield in cereals like wheat (as reflected by the harvest index), the influence of phloem transport rate and velocity is less clear.					
37303253	5	32	theme	little	1220:1225	arg1	change					1213:1218	xylem velocity change	1198:1218	xylem velocity change little with environmental conditions	1198:1255	Depending on the assumption made for phloem sap sucrose concentration, either phloem sap velocity or its proportionality coefficient to xylem velocity change little with environmental conditions.					
37303253	1	33	theme	activity	257:264	arg1	limitations					204:214	physiological limitations	190:214	physiological limitations of crop yield, along with photosynthetic activity or water use efficiency	190:288	Phloem sap transport, velocity and allocation have been proposed to play a role in physiological limitations of crop yield, along with photosynthetic activity or water use efficiency.					
37303253	6	34	theme	values	1364:1369	arg1	range					1355:1359	a narrow range	1346:1359	a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions	1346:1471	Taken as a whole, phloem transport from leaves to grains seems to be homeostatic within a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions.					
37303253	5	35	theme	velocity	1204:1211	arg1	change					1213:1218	xylem velocity change	1198:1218	xylem velocity change little with environmental conditions	1198:1255	Depending on the assumption made for phloem sap sucrose concentration, either phloem sap velocity or its proportionality coefficient to xylem velocity change little with environmental conditions.					
37303253	4	36	theme	phloem	862:867	arg1	rate					887:890	phloem sucrose transport rate	862:890	phloem sucrose transport rate	862:890	Our results suggest that phloem sucrose transport rate follows the same relationship with phloem N transport regardless of irrigation conditions and cultivars, and seems to depend mostly on grain weight (i.e., mg per grain).					
37303253	6	37	theme	physiological	1416:1428	arg1	parameters					1430:1439	other plant physiological parameters	1404:1439	other plant physiological parameters across cultivars and conditions	1404:1471	Taken as a whole, phloem transport from leaves to grains seems to be homeostatic within a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions.					
37303253	5	38	theme	environmental	1232:1244	arg1	conditions					1246:1255	environmental conditions	1232:1255	environmental conditions	1232:1255	Depending on the assumption made for phloem sap sucrose concentration, either phloem sap velocity or its proportionality coefficient to xylem velocity change little with environmental conditions.					
37303253	0	39	theme	phloem	29:34	arg1	transport					36:44	phloem transport	29:44	phloem transport to grains	29:54	Revisiting yield in terms of phloem transport to grains suggests phloem sap movement might be homeostatic.					
37303253	1	40	theme	water	269:273	arg1	efficiency					279:288	water use efficiency	269:288	water use efficiency	269:288	Phloem sap transport, velocity and allocation have been proposed to play a role in physiological limitations of crop yield, along with photosynthetic activity or water use efficiency.					
37303253	4	41	theme	sucrose	869:875	arg1	rate					887:890	phloem sucrose transport rate	862:890	phloem sucrose transport rate	862:890	Our results suggest that phloem sucrose transport rate follows the same relationship with phloem N transport regardless of irrigation conditions and cultivars, and seems to depend mostly on grain weight (i.e., mg per grain).					
37303253	3	42	theme	carbon	584:589	arg1	composition					599:609	carbon isotope composition	584:609	carbon isotope composition	584:609	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	6	43	theme	relationships	1385:1397	arg1	range					1355:1359	a narrow range	1346:1359	a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions	1346:1471	Taken as a whole, phloem transport from leaves to grains seems to be homeostatic within a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions.					
37303253	4	44	theme	N	934:934	arg1	transport					936:944	phloem N transport	927:944	phloem N transport	927:944	Our results suggest that phloem sucrose transport rate follows the same relationship with phloem N transport regardless of irrigation conditions and cultivars, and seems to depend mostly on grain weight (i.e., mg per grain).					
37303253	1	45	theme	use	275:277	arg1	efficiency					279:288	water use efficiency	269:288	water use efficiency	269:288	Phloem sap transport, velocity and allocation have been proposed to play a role in physiological limitations of crop yield, along with photosynthetic activity or water use efficiency.					
37303253	2	46	theme	velocity	484:491	arg1	influence					445:453	the influence	441:453	the influence of phloem transport rate and velocity	441:491	Although there is clear evidence that carbon allocation to grains effectively drives yield in cereals like wheat (as reflected by the harvest index), the influence of phloem transport rate and velocity is less clear.					
37303253	5	47	with	little	1220:1225	arg1	conditions					1246:1255	environmental conditions	1232:1255	environmental conditions	1232:1255	Depending on the assumption made for phloem sap sucrose concentration, either phloem sap velocity or its proportionality coefficient to xylem velocity change little with environmental conditions.					
37303253	5	48	theme	phloem	1099:1104	arg1	concentration					1118:1130	phloem sap sucrose concentration	1099:1130	phloem sap sucrose concentration	1099:1130	Depending on the assumption made for phloem sap sucrose concentration, either phloem sap velocity or its proportionality coefficient to xylem velocity change little with environmental conditions.					
37303253	2	49	theme	harvest	425:431	arg1	index					433:437	the harvest index	421:437	the harvest index	421:437	Although there is clear evidence that carbon allocation to grains effectively drives yield in cereals like wheat (as reflected by the harvest index), the influence of phloem transport rate and velocity is less clear.					
37303253	1	50	theme	efficiency	279:288	arg1	limitations					204:214	physiological limitations	190:214	physiological limitations of crop yield, along with photosynthetic activity or water use efficiency	190:288	Phloem sap transport, velocity and allocation have been proposed to play a role in physiological limitations of crop yield, along with photosynthetic activity or water use efficiency.					
37303253	4	51	theme	same	904:907	arg1	relationship					909:920	the same relationship	900:920	the same relationship with phloem N transport	900:944	Our results suggest that phloem sucrose transport rate follows the same relationship with phloem N transport regardless of irrigation conditions and cultivars, and seems to depend mostly on grain weight (i.e., mg per grain).					
37303253	6	52	theme	narrow	1348:1353	arg1	range					1355:1359	a narrow range	1346:1359	a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions	1346:1471	Taken as a whole, phloem transport from leaves to grains seems to be homeostatic within a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions.					
37303253	0	53	theme	transport	36:44	arg1	terms					20:24	terms	20:24	terms of phloem transport to grains	20:54	Revisiting yield in terms of phloem transport to grains suggests phloem sap movement might be homeostatic.					
37303253	6	54	from	leaves	1298:1303	arg1	transport					1283:1291	phloem transport	1276:1291	phloem transport from leaves to grains	1276:1313	Taken as a whole, phloem transport from leaves to grains seems to be homeostatic within a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions.					
37303253	1	55	dep	along	231:235	arg1	with					237:240	with	237:240	with	237:240	Phloem sap transport, velocity and allocation have been proposed to play a role in physiological limitations of crop yield, along with photosynthetic activity or water use efficiency.					
37303253	4	56	with	relationship	909:920	arg1	transport					936:944	phloem N transport	927:944	phloem N transport	927:944	Our results suggest that phloem sucrose transport rate follows the same relationship with phloem N transport regardless of irrigation conditions and cultivars, and seems to depend mostly on grain weight (i.e., mg per grain).					
37303253	1	57	theme	Phloem	107:112	arg1	transport					118:126	Phloem sap transport	107:126	Phloem sap transport	107:126	Phloem sap transport, velocity and allocation have been proposed to play a role in physiological limitations of crop yield, along with photosynthetic activity or water use efficiency.					
37303253	3	58	theme	transport	787:795	arg1	terms					763:767	terms	763:767	terms of phloem sucrose transport	763:795	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	5	59	theme	phloem	1140:1145	arg1	velocity					1151:1158	phloem sap velocity	1140:1158	phloem sap velocity	1140:1158	Depending on the assumption made for phloem sap sucrose concentration, either phloem sap velocity or its proportionality coefficient to xylem velocity change little with environmental conditions.					
37303253	3	60	theme	nitrogen	612:619	arg1	content					621:627	nitrogen content	612:627	nitrogen content	612:627	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	6	61	theme	other	1404:1408	arg1	parameters					1430:1439	other plant physiological parameters	1404:1439	other plant physiological parameters across cultivars and conditions	1404:1471	Taken as a whole, phloem transport from leaves to grains seems to be homeostatic within a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions.					
37303253	4	62	dep	weight	1033:1038	arg1	mg					1047:1048	mg	1047:1048	mg per grain	1047:1058	Our results suggest that phloem sucrose transport rate follows the same relationship with phloem N transport regardless of irrigation conditions and cultivars, and seems to depend mostly on grain weight (i.e., mg per grain).					
37303253	1	63	theme	sap	114:116	arg1	transport					118:126	Phloem sap transport	107:126	Phloem sap transport	107:126	Phloem sap transport, velocity and allocation have been proposed to play a role in physiological limitations of crop yield, along with photosynthetic activity or water use efficiency.					
37303253	5	64	theme	xylem	1198:1202	arg1	change					1213:1218	xylem velocity change	1198:1218	xylem velocity change little with environmental conditions	1198:1255	Depending on the assumption made for phloem sap sucrose concentration, either phloem sap velocity or its proportionality coefficient to xylem velocity change little with environmental conditions.					
37303253	1	65	theme	physiological	190:202	arg1	limitations					204:214	physiological limitations	190:214	physiological limitations of crop yield, along with photosynthetic activity or water use efficiency	190:288	Phloem sap transport, velocity and allocation have been proposed to play a role in physiological limitations of crop yield, along with photosynthetic activity or water use efficiency.					
37303253	3	66	theme	isotope	591:597	arg1	composition					599:609	carbon isotope composition	584:609	carbon isotope composition	584:609	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	5	67	theme	sap	1106:1108	arg1	concentration					1118:1130	phloem sap sucrose concentration	1099:1130	phloem sap sucrose concentration	1099:1130	Depending on the assumption made for phloem sap sucrose concentration, either phloem sap velocity or its proportionality coefficient to xylem velocity change little with environmental conditions.					
37303253	4	68	theme	transport	877:885	arg1	rate					887:890	phloem sucrose transport rate	862:890	phloem sucrose transport rate	862:890	Our results suggest that phloem sucrose transport rate follows the same relationship with phloem N transport regardless of irrigation conditions and cultivars, and seems to depend mostly on grain weight (i.e., mg per grain).					
37303253	3	69	theme	phloem	772:777	arg1	transport					787:795	phloem sucrose transport	772:795	phloem sucrose transport	772:795	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	3	70	theme	grain	743:747	arg1	production					749:758	grain production	743:758	grain production	743:758	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	2	71	theme	clear	309:313	arg1	evidence					315:322	clear evidence that carbon allocation to grains effectively drives yield in cereals like wheat	309:402	clear evidence that carbon allocation to grains effectively drives yield in cereals like wheat	309:402	Although there is clear evidence that carbon allocation to grains effectively drives yield in cereals like wheat (as reflected by the harvest index), the influence of phloem transport rate and velocity is less clear.					
37303253	3	72	theme	published	546:554	arg1	data					556:559	previously published data	535:559	previously published data	535:559	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	6	73	theme	following	1375:1383	arg1	relationships					1385:1397	following relationships	1375:1397	following relationships	1375:1397	Taken as a whole, phloem transport from leaves to grains seems to be homeostatic within a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions.					
37303253	3	74	theme	sucrose	779:785	arg1	transport					787:795	phloem sucrose transport	772:795	phloem sucrose transport	772:795	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	5	75	theme	sap	1147:1149	arg1	velocity					1151:1158	phloem sap velocity	1140:1158	phloem sap velocity	1140:1158	Depending on the assumption made for phloem sap sucrose concentration, either phloem sap velocity or its proportionality coefficient to xylem velocity change little with environmental conditions.					
37303253	3	76	from	composition	599:609	arg1	cultivars					667:675	winter wheat cultivars	654:675	winter wheat cultivars grown across several sites with or without irrigation	654:729	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	3	77	theme	water	633:637	arg1	consumption					639:649	water consumption	633:649	water consumption	633:649	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	3	78	theme	data	556:559	arg1	advantage					522:530	advantage	522:530	advantage of previously published data	522:559	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	3	79	from	respiration	571:581	arg1	cultivars					667:675	winter wheat cultivars	654:675	winter wheat cultivars grown across several sites with or without irrigation	654:729	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	3	80	theme	xylem	814:818	arg1	transport					826:834	xylem water transport	814:834	xylem water transport	814:834	Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport.					
37303253	1	81	theme	crop	219:222	arg1	yield					224:228	crop yield	219:228	crop yield	219:228	Phloem sap transport, velocity and allocation have been proposed to play a role in physiological limitations of crop yield, along with photosynthetic activity or water use efficiency.					
36525573	14	0	theme	CLINICAL	1694:1701	arg1	QUESTION/LEVEL					1703:1716	CLINICAL QUESTION/LEVEL	1694:1716	CLINICAL QUESTION/LEVEL OF EVIDENCE Therapeutic	1694:1740	CLINICAL QUESTION/LEVEL OF EVIDENCE Therapeutic, III.					
36525573	1	1	theme	late-onset	154:163	arg1	events					186:191	late-onset inflammatory adverse events	154:191	late-onset inflammatory adverse events with soft-tissue fillers	154:216	BACKGROUND The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers depends primarily on the assumed causative factor: immunologic or bacterial.					
36525573	12	2	theme	CONCLUSIONS	1501:1511	arg1	Bacteria					1513:1520	CONCLUSIONS Bacteria	1501:1520	CONCLUSIONS Bacteria adherent to soft-tissue fillers or bacteremia	1501:1566	CONCLUSIONS Bacteria adherent to soft-tissue fillers or bacteremia probably play a causative role in adverse events.					
36525573	9	3	from	group	1223:1227	arg1	patients					1192:1199	patients	1192:1199	patients from the inflammation group	1192:1227	RESULTS A high level of Gram-positive bacteria was found in biopsies of soft-tissue fillers, predominantly in patients from the inflammation group.					
36525573	11	4	from	composition	1331:1341	arg1	skin					1368:1371	the skin	1364:1371	the skin	1364:1371	The composition of the microbiota on the skin differed markedly from that in the filler, indicating that contamination during the sampling process did not influence results.					
36525573	3	5	with	groups	502:507	arg1	needle					522:527	an 18-G needle	514:527	an 18-G needle	514:527	Biopsies were acquired from both groups with an 18-G needle.					
36525573	10	6	theme	injection	1306:1314	arg1	treatment					1316:1324	the primary filler injection treatment	1287:1324	the primary filler injection treatment	1287:1324	This suggests that these bacteria were introduced during the primary filler injection treatment.					
36525573	1	7	theme	inflammatory	165:176	arg1	events					186:191	late-onset inflammatory adverse events	154:191	late-onset inflammatory adverse events with soft-tissue fillers	154:216	BACKGROUND The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers depends primarily on the assumed causative factor: immunologic or bacterial.					
36525573	14	8	theme	Therapeutic	1730:1740	arg1	EVIDENCE					1721:1728	EVIDENCE Therapeutic	1721:1740	EVIDENCE Therapeutic	1721:1740	CLINICAL QUESTION/LEVEL OF EVIDENCE Therapeutic, III.					
36525573	8	9	from	microbiota	967:976	arg1	contamination					943:955	contamination	943:955	contamination from skin microbiota	943:976	To exclude contamination from skin microbiota, the authors compared the microbiota found on skin swabs with that found in the corresponding biopsies.					
36525573	9	10	theme	fillers	1166:1172	arg1	biopsies					1142:1149	biopsies	1142:1149	biopsies of soft-tissue fillers	1142:1172	RESULTS A high level of Gram-positive bacteria was found in biopsies of soft-tissue fillers, predominantly in patients from the inflammation group.					
36525573	6	11	theme	sequence	780:787	arg1	variations					789:798	length and sequence variations	769:798	variations	789:798	This is a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region.					
36525573	4	12	theme	skin	577:580	arg1	swabs					582:586	skin swabs	577:586	skin swabs for 25 of the 29 patients	577:612	Before taking the biopsy, the authors acquired skin swabs for 25 of the 29 patients.					
36525573	5	13	dep	method	658:663	arg1	detect					668:673	detect	668:673	to detect microbiota-was	665:688	The IS-pro method-a new and very sensitive method to detect microbiota-was used.					
36525573	5	14	theme	new	635:637	arg1	method					658:663	The IS-pro method-a new and very sensitive method	615:663	The IS-pro method-a new and very sensitive method to detect microbiota-was	615:688	The IS-pro method-a new and very sensitive method to detect microbiota-was used.					
36525573	1	15	from	algorithm	141:149	arg1	events					186:191	late-onset inflammatory adverse events	154:191	late-onset inflammatory adverse events with soft-tissue fillers	154:216	BACKGROUND The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers depends primarily on the assumed causative factor: immunologic or bacterial.					
36525573	2	16	theme	inflammatory	371:382	arg1	events					392:397	late-onset inflammatory adverse events	360:397	late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group)	360:466	METHODS The authors included 29 patients, 13 of whom experienced late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group).					
36525573	0	17	theme	Events	108:113	arg1	Etiology					43:50	the Etiology	39:50	the Etiology of Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events	39:113	Bacterial Contamination Is Involved in the Etiology of Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events.					
36525573	6	18	theme	length	769:774	arg1	variations					789:798	length and sequence variations	769:798	variations	789:798	This is a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region.					
36525573	3	19	theme	18-G	517:520	arg1	needle					522:527	an 18-G needle	514:527	an 18-G needle	514:527	Biopsies were acquired from both groups with an 18-G needle.					
36525573	1	20	theme	adverse	178:184	arg1	events					186:191	late-onset inflammatory adverse events	154:191	late-onset inflammatory adverse events with soft-tissue fillers	154:216	BACKGROUND The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers depends primarily on the assumed causative factor: immunologic or bacterial.					
36525573	0	21	theme	Adverse	100:106	arg1	Events					108:113	Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events	55:113	Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events	55:113	Bacterial Contamination Is Involved in the Etiology of Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events.					
36525573	10	22	theme	filler	1299:1304	arg1	treatment					1316:1324	the primary filler injection treatment	1287:1324	the primary filler injection treatment	1287:1324	This suggests that these bacteria were introduced during the primary filler injection treatment.					
36525573	9	23	theme	soft-tissue	1154:1164	arg1	fillers					1166:1172	soft-tissue fillers	1154:1172	soft-tissue fillers	1154:1172	RESULTS A high level of Gram-positive bacteria was found in biopsies of soft-tissue fillers, predominantly in patients from the inflammation group.					
36525573	1	24	dep	BACKGROUND	116:125	arg1	depends					218:224	depends	218:224	depends primarily on the assumed causative factor	218:266	BACKGROUND The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers depends primarily on the assumed causative factor: immunologic or bacterial.					
36525573	1	24	dep	BACKGROUND	116:125	arg1	immunologic					269:279	immunologic	269:279	immunologic	269:279	BACKGROUND The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers depends primarily on the assumed causative factor: immunologic or bacterial.					
36525573	1	24	dep	BACKGROUND	116:125	arg1	bacterial					284:292	bacterial	284:292	bacterial	284:292	BACKGROUND The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers depends primarily on the assumed causative factor: immunologic or bacterial.					
36525573	9	25	dep	RESULTS	1082:1088	arg1	found					1133:1137	found	1133:1137	found	1133:1137	RESULTS A high level of Gram-positive bacteria was found in biopsies of soft-tissue fillers, predominantly in patients from the inflammation group.					
36525573	10	26	theme	primary	1291:1297	arg1	treatment					1316:1324	the primary filler injection treatment	1287:1324	the primary filler injection treatment	1287:1324	This suggests that these bacteria were introduced during the primary filler injection treatment.					
36525573	11	27	theme	microbiota	1350:1359	arg1	composition					1331:1341	The composition	1327:1341	The composition of the microbiota on the skin	1327:1371	The composition of the microbiota on the skin differed markedly from that in the filler, indicating that contamination during the sampling process did not influence results.					
36525573	8	28	theme	skin	962:965	arg1	microbiota					967:976	skin microbiota	962:976	skin microbiota	962:976	To exclude contamination from skin microbiota, the authors compared the microbiota found on skin swabs with that found in the corresponding biopsies.					
36525573	0	29	theme	Bacterial	0:8	arg1	Contamination					10:22	Bacterial Contamination	0:22	Bacterial Contamination	0:22	Bacterial Contamination Is Involved in the Etiology of Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events.					
36525573	8	30	theme	skin	1024:1027	arg1	swabs					1029:1033	skin swabs	1024:1033	skin swabs	1024:1033	To exclude contamination from skin microbiota, the authors compared the microbiota found on skin swabs with that found in the corresponding biopsies.					
36525573	2	31	theme	patients	327:334	arg1	13					337:338	13	337:338	13	337:338	METHODS The authors included 29 patients, 13 of whom experienced late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group).					
36525573	2	31	theme	patients	327:334	arg1	patients					327:334	29 patients	324:334	29 patients	324:334	METHODS The authors included 29 patients, 13 of whom experienced late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group).					
36525573	14	32	theme	EVIDENCE	1721:1728	arg1	QUESTION/LEVEL					1703:1716	CLINICAL QUESTION/LEVEL	1694:1716	CLINICAL QUESTION/LEVEL OF EVIDENCE Therapeutic	1694:1740	CLINICAL QUESTION/LEVEL OF EVIDENCE Therapeutic, III.					
36525573	1	33	theme	soft-tissue	198:208	arg1	fillers					210:216	soft-tissue fillers	198:216	soft-tissue fillers	198:216	BACKGROUND The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers depends primarily on the assumed causative factor: immunologic or bacterial.					
36525573	12	34	theme	causative	1584:1592	arg1	role					1594:1597	a causative role	1582:1597	a causative role	1582:1597	CONCLUSIONS Bacteria adherent to soft-tissue fillers or bacteremia probably play a causative role in adverse events.					
36525573	13	35	theme	samples	1635:1641	arg1	Contamination					1618:1630	Contamination	1618:1630	Contamination of samples in the biopsies with skin microbiota	1618:1678	Contamination of samples in the biopsies with skin microbiota was excluded.					
36525573	6	36	theme	reaction	741:748	arg1	technique					750:758	a novel broad-range polymerase chain reaction technique	704:758	a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region	704:845	This is a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region.					
36525573	6	36	theme	reaction	741:748	arg1	This					696:699	This	696:699	This	696:699	This is a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region.					
36525573	0	37	dep	Filler	67:72	arg1	Inflammatory					87:98	Inflammatory	87:98	Inflammatory	87:98	Bacterial Contamination Is Involved in the Etiology of Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events.					
36525573	0	37	dep	Filler	67:72	arg1	Late-Onset					75:84	Late-Onset	75:84	Late-Onset	75:84	Bacterial Contamination Is Involved in the Etiology of Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events.					
36525573	11	38	theme	sampling	1457:1464	arg1	process					1466:1472	the sampling process	1453:1472	the sampling process	1453:1472	The composition of the microbiota on the skin differed markedly from that in the filler, indicating that contamination during the sampling process did not influence results.					
36525573	8	39	located	found	1015:1019	arg2	microbiota					1004:1013	the microbiota	1000:1013	the microbiota found on skin swabs	1000:1033	To exclude contamination from skin microbiota, the authors compared the microbiota found on skin swabs with that found in the corresponding biopsies.					
36525573	8	39	located	found	1015:1019	arg1	swabs					1029:1033	skin swabs	1024:1033	skin swabs	1024:1033	To exclude contamination from skin microbiota, the authors compared the microbiota found on skin swabs with that found in the corresponding biopsies.					
36525573	9	40	located	found	1133:1137	arg1	biopsies					1142:1149	biopsies	1142:1149	biopsies of soft-tissue fillers	1142:1172	RESULTS A high level of Gram-positive bacteria was found in biopsies of soft-tissue fillers, predominantly in patients from the inflammation group.					
36525573	9	40	located	found	1133:1137	arg2	level					1097:1101	A high level	1090:1101	A high level of Gram-positive bacteria	1090:1127	RESULTS A high level of Gram-positive bacteria was found in biopsies of soft-tissue fillers, predominantly in patients from the inflammation group.					
36525573	9	40	located	found	1133:1137	arg1	patients					1192:1199	patients	1192:1199	patients from the inflammation group	1192:1227	RESULTS A high level of Gram-positive bacteria was found in biopsies of soft-tissue fillers, predominantly in patients from the inflammation group.					
36525573	12	41	theme	adverse	1602:1608	arg1	events					1610:1615	adverse events	1602:1615	adverse events	1602:1615	CONCLUSIONS Bacteria adherent to soft-tissue fillers or bacteremia probably play a causative role in adverse events.					
36525573	9	42	theme	high	1092:1095	arg1	level					1097:1101	A high level	1090:1101	A high level of Gram-positive bacteria	1090:1127	RESULTS A high level of Gram-positive bacteria was found in biopsies of soft-tissue fillers, predominantly in patients from the inflammation group.					
36525573	2	43	theme	late-onset	360:369	arg1	events					392:397	late-onset inflammatory adverse events	360:397	late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group)	360:466	METHODS The authors included 29 patients, 13 of whom experienced late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group).					
36525573	7	44	theme	species	917:923	arg1	level					925:929	species level	917:929	species level	917:929	IS-pro can detect bacteria at low abundances and identify them up to species level.					
36525573	5	45	used	used	690:693	arg2	method					658:663	The IS-pro method-a new and very sensitive method	615:663	The IS-pro method-a new and very sensitive method to detect microbiota-was	615:688	The IS-pro method-a new and very sensitive method to detect microbiota-was used.					
36525573	6	46	theme	interspacer	828:838	arg1	region					840:845	23S ribosomal interspacer region	814:845	23S ribosomal interspacer region	814:845	This is a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region.					
36525573	2	47	theme	inflammatory	411:422	arg1	fillers					402:408	fillers	402:408	fillers (inflammatory group)	402:429	METHODS The authors included 29 patients, 13 of whom experienced late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group).					
36525573	2	47	theme	inflammatory	411:422	arg1	group					424:428	inflammatory group	411:428	inflammatory group	411:428	METHODS The authors included 29 patients, 13 of whom experienced late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group).					
36525573	14	48	dep	III	1743:1745	arg1	QUESTION/LEVEL					1703:1716	CLINICAL QUESTION/LEVEL	1694:1716	CLINICAL QUESTION/LEVEL OF EVIDENCE Therapeutic	1694:1740	CLINICAL QUESTION/LEVEL OF EVIDENCE Therapeutic, III.					
36525573	6	49	theme	ribosomal	818:826	arg1	region					840:845	23S ribosomal interspacer region	814:845	23S ribosomal interspacer region	814:845	This is a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region.					
36525573	6	50	theme	chain	735:739	arg1	reaction					741:748	novel broad-range polymerase chain reaction	706:748	a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region	704:845	This is a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region.					
36525573	4	51	theme	patients	605:612	arg1	patients					605:612	the 29 patients	598:612	the 29 patients	598:612	Before taking the biopsy, the authors acquired skin swabs for 25 of the 29 patients.					
36525573	4	51	theme	patients	605:612	arg1	25					592:593	25	592:593	25	592:593	Before taking the biopsy, the authors acquired skin swabs for 25 of the 29 patients.					
36525573	8	52	theme	corresponding	1058:1070	arg1	biopsies					1072:1079	the corresponding biopsies	1054:1079	the corresponding biopsies	1054:1079	To exclude contamination from skin microbiota, the authors compared the microbiota found on skin swabs with that found in the corresponding biopsies.					
36525573	6	53	theme	23S	814:816	arg1	region					840:845	23S ribosomal interspacer region	814:845	23S ribosomal interspacer region	814:845	This is a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region.					
36525573	6	54	theme	polymerase	724:733	arg1	reaction					741:748	novel broad-range polymerase chain reaction	706:748	a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region	704:845	This is a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region.					
36525573	12	55	theme	adherent	1522:1529	arg1	Bacteria					1513:1520	CONCLUSIONS Bacteria	1501:1520	CONCLUSIONS Bacteria adherent to soft-tissue fillers or bacteremia	1501:1566	CONCLUSIONS Bacteria adherent to soft-tissue fillers or bacteremia probably play a causative role in adverse events.					
36525573	2	56	theme	reference	451:459	arg1	group					461:465	reference group	451:465	reference group	451:465	METHODS The authors included 29 patients, 13 of whom experienced late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group).					
36525573	2	56	theme	reference	451:459	arg1	16					435:436	16	435:436	16	435:436	METHODS The authors included 29 patients, 13 of whom experienced late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group).					
36525573	9	57	theme	inflammation	1210:1221	arg1	group					1223:1227	the inflammation group	1206:1227	the inflammation group	1206:1227	RESULTS A high level of Gram-positive bacteria was found in biopsies of soft-tissue fillers, predominantly in patients from the inflammation group.					
36525573	13	58	theme	skin	1664:1667	arg1	microbiota					1669:1678	skin microbiota	1664:1678	skin microbiota	1664:1678	Contamination of samples in the biopsies with skin microbiota was excluded.					
36525573	6	59	theme	broad-range	712:722	arg1	reaction					741:748	novel broad-range polymerase chain reaction	706:748	a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region	704:845	This is a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region.					
36525573	7	60	theme	low	878:880	arg1	abundances					882:891	low abundances	878:891	low abundances	878:891	IS-pro can detect bacteria at low abundances and identify them up to species level.					
36525573	5	61	theme	sensitive	648:656	arg1	method					658:663	The IS-pro method-a new and very sensitive method	615:663	The IS-pro method-a new and very sensitive method to detect microbiota-was	615:688	The IS-pro method-a new and very sensitive method to detect microbiota-was used.					
36525573	6	62	theme	16S	807:809	arg1	variations					789:798	length and sequence variations	769:798	variations	789:798	This is a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region.					
36525573	6	63	theme	novel	706:710	arg1	reaction					741:748	novel broad-range polymerase chain reaction	706:748	a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region	704:845	This is a novel broad-range polymerase chain reaction technique based on length and sequence variations of the 16S to 23S ribosomal interspacer region.					
36525573	8	64	located	found	1045:1049	arg1	biopsies					1072:1079	the corresponding biopsies	1054:1079	the corresponding biopsies	1054:1079	To exclude contamination from skin microbiota, the authors compared the microbiota found on skin swabs with that found in the corresponding biopsies.					
36525573	8	64	located	found	1045:1049	arg2	that					1040:1043	that	1040:1043	that	1040:1043	To exclude contamination from skin microbiota, the authors compared the microbiota found on skin swabs with that found in the corresponding biopsies.					
36525573	12	65	theme	soft-tissue	1534:1544	arg1	fillers					1546:1552	soft-tissue fillers	1534:1552	soft-tissue fillers	1534:1552	CONCLUSIONS Bacteria adherent to soft-tissue fillers or bacteremia probably play a causative role in adverse events.					
36525573	1	66	with	events	186:191	arg1	fillers					210:216	soft-tissue fillers	198:216	soft-tissue fillers	198:216	BACKGROUND The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers depends primarily on the assumed causative factor: immunologic or bacterial.					
36525573	13	67	from	Contamination	1618:1630	arg1	biopsies					1650:1657	the biopsies	1646:1657	the biopsies	1646:1657	Contamination of samples in the biopsies with skin microbiota was excluded.					
36525573	1	68	theme	treatment	131:139	arg1	algorithm					141:149	The treatment algorithm	127:149	The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers	127:216	BACKGROUND The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers depends primarily on the assumed causative factor: immunologic or bacterial.					
36525573	7	69	dep	level	925:929	arg1	up					911:912	up	911:912	up	911:912	IS-pro can detect bacteria at low abundances and identify them up to species level.					
36525573	1	70	theme	assumed	243:249	arg1	factor					261:266	the assumed causative factor	239:266	the assumed causative factor	239:266	BACKGROUND The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers depends primarily on the assumed causative factor: immunologic or bacterial.					
36525573	0	71	theme	Filler	67:72	arg1	Events					108:113	Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events	55:113	Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events	55:113	Bacterial Contamination Is Involved in the Etiology of Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events.					
36525573	13	72	with	Contamination	1618:1630	arg1	microbiota					1669:1678	skin microbiota	1664:1678	skin microbiota	1664:1678	Contamination of samples in the biopsies with skin microbiota was excluded.					
36525573	9	73	theme	Gram-positive	1106:1118	arg1	bacteria					1120:1127	Gram-positive bacteria	1106:1127	Gram-positive bacteria	1106:1127	RESULTS A high level of Gram-positive bacteria was found in biopsies of soft-tissue fillers, predominantly in patients from the inflammation group.					
36525573	2	74	dep	METHODS	295:301	arg1	included					315:322	included	315:322	included 29 patients, 13 of whom experienced late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group)	315:466	METHODS The authors included 29 patients, 13 of whom experienced late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group).					
36525573	1	75	theme	causative	251:259	arg1	factor					261:266	the assumed causative factor	239:266	the assumed causative factor	239:266	BACKGROUND The treatment algorithm in late-onset inflammatory adverse events with soft-tissue fillers depends primarily on the assumed causative factor: immunologic or bacterial.					
36525573	0	76	theme	Soft-Tissue	55:65	arg1	Events					108:113	Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events	55:113	Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events	55:113	Bacterial Contamination Is Involved in the Etiology of Soft-Tissue Filler, Late-Onset, Inflammatory Adverse Events.					
36525573	9	77	theme	bacteria	1120:1127	arg1	level					1097:1101	A high level	1090:1101	A high level of Gram-positive bacteria	1090:1127	RESULTS A high level of Gram-positive bacteria was found in biopsies of soft-tissue fillers, predominantly in patients from the inflammation group.					
36525573	2	78	theme	adverse	384:390	arg1	events					392:397	late-onset inflammatory adverse events	360:397	late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group)	360:466	METHODS The authors included 29 patients, 13 of whom experienced late-onset inflammatory adverse events to fillers (inflammatory group) and 16 who did not (reference group).					
35040049	8	0	theme	fresh	931:935	arg1	media					945:949	fresh culture media	931:949	fresh culture media	931:949	C. vulgaris in flocs could successfully regrow in fresh culture media.					
35040049	7	1	dep	have	784:787	arg1	both					779:782	both	779:782	both	779:782	Chitosan and polyacrylamide both have no negative effect on biomass composition, including protein, carbohydrate, and carotenoid.					
35040049	5	2	theme	flocculation	452:463	arg1	FE					477:478	FE	477:478	FE	477:478	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	5	2	theme	flocculation	452:463	arg1	efficiency					465:474	flocculation efficiency	452:474	flocculation efficiency (FE)	452:479	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	5	2	theme	flocculation	452:463	arg1	%					494:494	98.10 ± 1.06%	482:494	98.10 ± 1.06%	482:494	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	9	3	from	impact	1004:1009	arg1	growth					1019:1024	cell growth	1014:1024	cell growth	1014:1024	The residual culture media was recycled with little impact on cell growth.					
35040049	6	4	dep	FE	700:701	arg1	%					698:698	polyacrylamide keep > 90%	674:698	polyacrylamide keep > 90%	674:698	Chitosan resulted flocs settled more quickly than polyacrylamide, while polyacrylamide keep > 90% FE in a wider pH range (7-10) than chitosan (7-8).					
35040049	6	5	theme	pH	714:715	arg1	7-10					724:727	7-10	724:727	7-10	724:727	Chitosan resulted flocs settled more quickly than polyacrylamide, while polyacrylamide keep > 90% FE in a wider pH range (7-10) than chitosan (7-8).					
35040049	6	5	theme	pH	714:715	arg1	range					717:721	a wider pH range	706:721	a wider pH range (7-10) than chitosan (7-8)	706:748	Chitosan resulted flocs settled more quickly than polyacrylamide, while polyacrylamide keep > 90% FE in a wider pH range (7-10) than chitosan (7-8).					
35040049	3	6	from	condition	315:323	arg1	research					251:258	this research	246:258	this research	246:258	In this research, chitosan and polyacrylamide were optimized harvesting condition for Chlorella vulgaris.					
35040049	1	7	theme	Microalgal	89:98	arg1	biomass					100:106	Microalgal biomass	89:106	Microalgal biomass	89:106	Microalgal biomass is an emerging source of renewable energy and health-related compounds.					
35040049	1	7	theme	Microalgal	89:98	arg1	source					123:128	an emerging source	111:128	an emerging source of renewable energy and health-related compounds	111:177	Microalgal biomass is an emerging source of renewable energy and health-related compounds.					
35040049	6	8	theme	wider	708:712	arg1	7-10					724:727	7-10	724:727	7-10	724:727	Chitosan resulted flocs settled more quickly than polyacrylamide, while polyacrylamide keep > 90% FE in a wider pH range (7-10) than chitosan (7-8).					
35040049	6	8	theme	wider	708:712	arg1	range					717:721	a wider pH range	706:721	a wider pH range (7-10) than chitosan (7-8)	706:748	Chitosan resulted flocs settled more quickly than polyacrylamide, while polyacrylamide keep > 90% FE in a wider pH range (7-10) than chitosan (7-8).					
35040049	0	9	theme	Chlorella	13:21	arg1	biomass					32:38	High-quality Chlorella vulgaris biomass	0:38	High-quality Chlorella vulgaris biomass	0:38	High-quality Chlorella vulgaris biomass harvesting through chitosan and polyacrylamid2e.					
35040049	8	10	theme	C.	881:882	arg1	vulgaris					884:891	C. vulgaris	881:891	C. vulgaris in flocs	881:900	C. vulgaris in flocs could successfully regrow in fresh culture media.					
35040049	5	11	theme	high-dose	520:528	arg1	polyacrylamide					540:553	high-dose (25 mg/L) polyacrylamide	520:553	high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris	520:599	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	5	11	theme	high-dose	520:528	arg1	%					571:571	FE 94.57 ± 0.55%	556:571	FE 94.57 ± 0.55%	556:571	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	0	12	theme	High-quality	0:11	arg1	biomass					32:38	High-quality Chlorella vulgaris biomass	0:38	High-quality Chlorella vulgaris biomass	0:38	High-quality Chlorella vulgaris biomass harvesting through chitosan and polyacrylamid2e.					
35040049	5	13	theme	harvesting	578:587	arg1	vulgaris					592:599	harvesting C. vulgaris	578:599	harvesting C. vulgaris	578:599	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	5	14	dep	chitosan	442:449	arg1	FE					477:478	FE	477:478	FE	477:478	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	5	14	dep	chitosan	442:449	arg1	efficiency					465:474	flocculation efficiency	452:474	flocculation efficiency (FE)	452:479	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	5	14	dep	chitosan	442:449	arg1	%					494:494	98.10 ± 1.06%	482:494	98.10 ± 1.06%	482:494	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	3	15	theme	harvesting	304:313	arg1	condition					315:323	optimized harvesting condition	294:323	optimized harvesting condition for Chlorella vulgaris	294:346	In this research, chitosan and polyacrylamide were optimized harvesting condition for Chlorella vulgaris.					
35040049	5	16	theme	C.	589:590	arg1	vulgaris					592:599	harvesting C. vulgaris	578:599	harvesting C. vulgaris	578:599	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	1	17	theme	emerging	114:121	arg1	biomass					100:106	Microalgal biomass	89:106	Microalgal biomass	89:106	Microalgal biomass is an emerging source of renewable energy and health-related compounds.					
35040049	1	17	theme	emerging	114:121	arg1	source					123:128	an emerging source	111:128	an emerging source of renewable energy and health-related compounds	111:177	Microalgal biomass is an emerging source of renewable energy and health-related compounds.					
35040049	7	18	theme	biomass	811:817	arg1	composition					819:829	biomass composition	811:829	biomass composition	811:829	Chitosan and polyacrylamide both have no negative effect on biomass composition, including protein, carbohydrate, and carotenoid.					
35040049	0	19	theme	vulgaris	23:30	arg1	biomass					32:38	High-quality Chlorella vulgaris biomass	0:38	High-quality Chlorella vulgaris biomass	0:38	High-quality Chlorella vulgaris biomass harvesting through chitosan and polyacrylamid2e.					
35040049	10	20	theme	high-quality	1100:1111	arg1	biomass					1124:1130	high-quality microalgal biomass	1100:1130	high-quality microalgal biomass	1100:1130	All the results suggested that chitosan and polyacrylamide could harvest high-quality microalgal biomass.					
35040049	2	21	theme	microalgae	203:212	arg1	techno-economic					219:233	techno-economic	219:233	techno-economic	219:233	However, harvesting of microalgae is a techno-economic hinder.					
35040049	2	21	theme	microalgae	203:212	arg1	harvesting					189:198	harvesting	189:198	harvesting of microalgae	189:212	However, harvesting of microalgae is a techno-economic hinder.					
35040049	7	22	contain	have	784:787	arg1	Chitosan					751:758	Chitosan	751:758	Chitosan	751:758	Chitosan and polyacrylamide both have no negative effect on biomass composition, including protein, carbohydrate, and carotenoid.					
35040049	7	22	contain	have	784:787	arg1	polyacrylamide					764:777	polyacrylamide	764:777	polyacrylamide	764:777	Chitosan and polyacrylamide both have no negative effect on biomass composition, including protein, carbohydrate, and carotenoid.					
35040049	7	22	contain	have	784:787	arg2	effect					801:806	no negative effect	789:806	no negative effect	789:806	Chitosan and polyacrylamide both have no negative effect on biomass composition, including protein, carbohydrate, and carotenoid.					
35040049	7	23	theme	negative	792:799	arg1	effect					801:806	no negative effect	789:806	no negative effect	789:806	Chitosan and polyacrylamide both have no negative effect on biomass composition, including protein, carbohydrate, and carotenoid.					
35040049	9	24	theme	culture	965:971	arg1	media					973:977	The residual culture media	952:977	The residual culture media	952:977	The residual culture media was recycled with little impact on cell growth.					
35040049	6	25	theme	polyacrylamide	674:687	arg1	%					698:698	polyacrylamide keep > 90%	674:698	polyacrylamide keep > 90%	674:698	Chitosan resulted flocs settled more quickly than polyacrylamide, while polyacrylamide keep > 90% FE in a wider pH range (7-10) than chitosan (7-8).					
35040049	3	26	theme	optimized	294:302	arg1	condition					315:323	optimized harvesting condition	294:323	optimized harvesting condition for Chlorella vulgaris	294:346	In this research, chitosan and polyacrylamide were optimized harvesting condition for Chlorella vulgaris.					
35040049	5	27	dep	Low-dose	423:430	arg1	10 mg/L					433:439	10 mg/L	433:439	10 mg/L	433:439	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	10	28	theme	microalgal	1113:1122	arg1	biomass					1124:1130	high-quality microalgal biomass	1100:1130	high-quality microalgal biomass	1100:1130	All the results suggested that chitosan and polyacrylamide could harvest high-quality microalgal biomass.					
35040049	5	29	theme	FE	556:557	arg1	polyacrylamide					540:553	high-dose (25 mg/L) polyacrylamide	520:553	high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris	520:599	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	5	29	theme	FE	556:557	arg1	%					571:571	FE 94.57 ± 0.55%	556:571	FE 94.57 ± 0.55%	556:571	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	1	30	theme	renewable	133:141	arg1	energy					143:148	renewable energy	133:148	renewable energy	133:148	Microalgal biomass is an emerging source of renewable energy and health-related compounds.					
35040049	8	31	from	vulgaris	884:891	arg1	flocs					896:900	flocs	896:900	flocs	896:900	C. vulgaris in flocs could successfully regrow in fresh culture media.					
35040049	1	32	theme	energy	143:148	arg1	biomass					100:106	Microalgal biomass	89:106	Microalgal biomass	89:106	Microalgal biomass is an emerging source of renewable energy and health-related compounds.					
35040049	1	32	theme	energy	143:148	arg1	source					123:128	an emerging source	111:128	an emerging source of renewable energy and health-related compounds	111:177	Microalgal biomass is an emerging source of renewable energy and health-related compounds.					
35040049	6	33	from	FE	700:701	arg1	7-10					724:727	7-10	724:727	7-10	724:727	Chitosan resulted flocs settled more quickly than polyacrylamide, while polyacrylamide keep > 90% FE in a wider pH range (7-10) than chitosan (7-8).					
35040049	6	33	from	FE	700:701	arg1	range					717:721	a wider pH range	706:721	a wider pH range (7-10) than chitosan (7-8)	706:748	Chitosan resulted flocs settled more quickly than polyacrylamide, while polyacrylamide keep > 90% FE in a wider pH range (7-10) than chitosan (7-8).					
35040049	5	34	theme	Low-dose	423:430	arg1	chitosan					442:449	Low-dose (10 mg/L) chitosan	423:449	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%)	423:495	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	3	35	theme	Chlorella	329:337	arg1	vulgaris					339:346	Chlorella vulgaris	329:346	Chlorella vulgaris	329:346	In this research, chitosan and polyacrylamide were optimized harvesting condition for Chlorella vulgaris.					
35040049	4	36	from	300 rpm	361:367	arg1	Stirring					349:356	Stirring	349:356	Stirring at 300 rpm for 2 min	349:377	Stirring at 300 rpm for 2 min is optimum for chitosan and polyacrylamide.					
35040049	6	37	from	range	717:721	arg1	FE					700:701	FE	700:701	FE	700:701	Chitosan resulted flocs settled more quickly than polyacrylamide, while polyacrylamide keep > 90% FE in a wider pH range (7-10) than chitosan (7-8).					
35040049	5	38	theme	25 mg/L	531:537	arg1	polyacrylamide					540:553	high-dose (25 mg/L) polyacrylamide	520:553	high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris	520:599	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	5	38	theme	25 mg/L	531:537	arg1	%					571:571	FE 94.57 ± 0.55%	556:571	FE 94.57 ± 0.55%	556:571	Low-dose (10 mg/L) chitosan (flocculation efficiency (FE), 98.10 ± 1.06%) is more efficient than high-dose (25 mg/L) polyacrylamide (FE 94.57 ± 0.55%) for harvesting C. vulgaris.					
35040049	1	39	theme	health-related	154:167	arg1	compounds					169:177	health-related compounds	154:177	health-related compounds	154:177	Microalgal biomass is an emerging source of renewable energy and health-related compounds.					
35040049	9	40	theme	little	997:1002	arg1	impact					1004:1009	little impact	997:1009	little impact on cell growth	997:1024	The residual culture media was recycled with little impact on cell growth.					
35040049	9	41	theme	residual	956:963	arg1	media					973:977	The residual culture media	952:977	The residual culture media	952:977	The residual culture media was recycled with little impact on cell growth.					
35040049	8	42	theme	culture	937:943	arg1	media					945:949	fresh culture media	931:949	fresh culture media	931:949	C. vulgaris in flocs could successfully regrow in fresh culture media.					
35040049	1	43	theme	compounds	169:177	arg1	biomass					100:106	Microalgal biomass	89:106	Microalgal biomass	89:106	Microalgal biomass is an emerging source of renewable energy and health-related compounds.					
35040049	1	43	theme	compounds	169:177	arg1	source					123:128	an emerging source	111:128	an emerging source of renewable energy and health-related compounds	111:177	Microalgal biomass is an emerging source of renewable energy and health-related compounds.					
35040049	9	44	theme	cell	1014:1017	arg1	growth					1019:1024	cell growth	1014:1024	cell growth	1014:1024	The residual culture media was recycled with little impact on cell growth.					
35040049	2	45	dep	techno-economic	219:233	arg1	hinder					235:240	hinder	235:240	hinder	235:240	However, harvesting of microalgae is a techno-economic hinder.					
36499070	5	0	theme	higher	659:664	arg1	quantity					666:673	A higher quantity	657:673	A higher quantity of CMD	657:680	A higher quantity of CMD leads to a thicker coating and a reduced superparamagnetic core size with decreasing magnetization.					
36499070	6	1	theme	CMD	807:809	arg1	L-1					800:802	12.5-25.0 g L-1	788:802	12.5-25.0 g L-1 of CMD	788:809	Above 12.5-25.0 g L-1 of CMD, the particles are colloidally stable.					
36499070	3	2	theme	CMD	549:551	arg1	thickness					561:569	the CMD coating thickness	545:569	the CMD coating thickness	545:569	This study reveals how the CMD coating thickness can influence these particle properties.					
36499070	1	3	theme	oxide	194:198	arg1	nanoparticles					200:212	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles	154:212	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs)	154:219	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs) are of great interest in nanomedicine, especially for applications in drug delivery.					
36499070	1	3	theme	oxide	194:198	arg1	IONs					215:218	IONs	215:218	IONs	215:218	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs) are of great interest in nanomedicine, especially for applications in drug delivery.					
36499070	9	4	from	coupling	1287:1294	arg1	comparison					1395:1404	comparison	1395:1404	comparison with the adsorption method (24% drug loading, 28% efficiency)	1395:1466	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	7	5	dep	diameters	886:894	arg1	diameters					886:894	hydrodynamic diameters	873:894	hydrodynamic diameters &lt; 100 nm and a good cell viability	873:932	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	7	5	dep	diameters	886:894	arg1	viability					924:932	a good cell viability	912:932	a good cell viability	912:932	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	7	5	dep	diameters	886:894	arg1	nm					905:906	&lt; 100 nm	896:906	&lt; 100 nm	896:906	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	10	6	theme	inhibitory	1531:1540	arg1	concentration					1542:1554	a minimal inhibitory concentration	1521:1554	a minimal inhibitory concentration of 1.13 µM (adsorption) and 1.70 µM (covalent)	1521:1601	The systems showed high antimicrobial activity with a minimal inhibitory concentration of 1.13 µM (adsorption) and 1.70 µM (covalent).					
36499070	1	7	from	applications	275:286	arg1	delivery					296:303	drug delivery	291:303	drug delivery	291:303	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs) are of great interest in nanomedicine, especially for applications in drug delivery.					
36499070	9	8	theme	peptide	1204:1210	arg1	lasioglossin					1212:1223	the antimicrobial peptide lasioglossin	1186:1223	the antimicrobial peptide lasioglossin	1186:1223	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	9	9	theme	24	1434:1435	arg1	%					1436:1436	%	1436:1436	%	1436:1436	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	5	10	theme	superparamagnetic	723:739	arg1	size					746:749	a reduced superparamagnetic core size	713:749	a reduced superparamagnetic core size	713:749	A higher quantity of CMD leads to a thicker coating and a reduced superparamagnetic core size with decreasing magnetization.					
36499070	7	11	theme	systems	1040:1046	arg1	characteristics					1007:1021	two of the most critical characteristics	982:1021	two of the most critical characteristics of drug delivery systems	982:1046	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	9	12	theme	drug	1438:1441	arg1	loading					1443:1449	24% drug loading	1434:1449	24% drug loading	1434:1449	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	9	12	theme	drug	1438:1441	arg1	efficiency					1456:1465	28% efficiency	1452:1465	28% efficiency	1452:1465	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	7	13	with	contact	937:943	arg1	cells					964:968	smooth muscle cells	950:968	smooth muscle cells	950:968	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	9	14	theme	EDC/NHS	1305:1311	arg1	method					1385:1390	the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method	1301:1390	the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method	1301:1390	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	10	15	theme	µM	1564:1565	arg1	concentration					1542:1554	a minimal inhibitory concentration	1521:1554	a minimal inhibitory concentration of 1.13 µM (adsorption) and 1.70 µM (covalent)	1521:1601	The systems showed high antimicrobial activity with a minimal inhibitory concentration of 1.13 µM (adsorption) and 1.70 µM (covalent).					
36499070	7	16	theme	drug	1026:1029	arg1	systems					1040:1046	drug delivery systems	1026:1046	drug delivery systems	1026:1046	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	9	17	theme	excellent	1232:1240	arg1	efficiency					1242:1251	an excellent efficiency	1229:1251	an excellent efficiency (82.3%)	1229:1259	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	9	17	theme	excellent	1232:1240	arg1	%					1258:1258	82.3%	1254:1258	82.3%	1254:1258	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	3	18	theme	particle	591:598	arg1	properties					600:609	these particle properties	585:609	these particle properties	585:609	This study reveals how the CMD coating thickness can influence these particle properties.					
36499070	11	19	theme	drug	1696:1699	arg1	carrier					1701:1707	a magnetically controllable drug carrier	1668:1707	a magnetically controllable drug carrier	1668:1707	This system successfully combines an antimicrobial peptide with a magnetically controllable drug carrier.					
36499070	1	20	from	interest	234:241	arg1	nanomedicine					246:257	nanomedicine	246:257	nanomedicine	246:257	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs) are of great interest in nanomedicine, especially for applications in drug delivery.					
36499070	9	21	theme	%	1454:1454	arg1	loading					1443:1449	24% drug loading	1434:1449	24% drug loading	1434:1449	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	9	21	theme	%	1454:1454	arg1	efficiency					1456:1465	28% efficiency	1452:1465	28% efficiency	1452:1465	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	2	22	theme	drug	507:510	arg1	activity					512:519	drug activity	507:519	drug activity	507:519	To develop a magnetically controlled drug delivery system, many factors must be considered, including the composition, surface properties, size and agglomeration, magnetization, cytocompatibility, and drug activity.					
36499070	7	23	theme	muscle	957:962	arg1	cells					964:968	smooth muscle cells	950:968	smooth muscle cells	950:968	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	1	24	theme	-coated	181:187	arg1	nanoparticles					200:212	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles	154:212	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs)	154:219	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs) are of great interest in nanomedicine, especially for applications in drug delivery.					
36499070	1	24	theme	-coated	181:187	arg1	IONs					215:218	IONs	215:218	IONs	215:218	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs) are of great interest in nanomedicine, especially for applications in drug delivery.					
36499070	9	25	dep	EDC/NHS	1305:1311	arg1	N-ethyl-N'-					1314:1324	N-ethyl-N'-	1314:1324	N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide	1314:1382	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	0	26	theme	Coating	108:114	arg1	Thickness					116:124	the Coating Thickness	104:124	the Coating Thickness	104:124	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.					
36499070	8	27	from	impact	1083:1088	arg1	behavior					1130:1137	the magnetophoretic behavior	1110:1137	the magnetophoretic behavior	1110:1137	New insights into the significant impact of agglomeration on the magnetophoretic behavior are shown.					
36499070	7	28	theme	cell	919:922	arg1	diameters					886:894	hydrodynamic diameters	873:894	hydrodynamic diameters &lt; 100 nm and a good cell viability	873:932	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	7	28	theme	cell	919:922	arg1	viability					924:932	a good cell viability	912:932	a good cell viability	912:932	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	9	29	with	efficiency	1242:1251	arg1	lasioglossin					1212:1223	the antimicrobial peptide lasioglossin	1186:1223	the antimicrobial peptide lasioglossin	1186:1223	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	0	30	theme	Carboxymethyl-Dextran-Coated	0:27	arg1	Nanoparticles					58:70	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles	0:70	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.	0:152	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.					
36499070	2	31	theme	surface	425:431	arg1	properties					433:442	surface properties	425:442	surface properties	425:442	To develop a magnetically controlled drug delivery system, many factors must be considered, including the composition, surface properties, size and agglomeration, magnetization, cytocompatibility, and drug activity.					
36499070	0	32	theme	Iron	47:50	arg1	Nanoparticles					58:70	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles	0:70	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.	0:152	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.					
36499070	9	33	with	comparison	1395:1404	arg1	method					1426:1431	the adsorption method	1411:1431	the adsorption method (24% drug loading, 28% efficiency)	1411:1466	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	0	34	theme	Drug	76:79	arg1	Delivery					81:88	Drug Delivery	76:88	Drug Delivery	76:88	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.					
36499070	9	35	with	loadings	1166:1173	arg1	lasioglossin					1212:1223	the antimicrobial peptide lasioglossin	1186:1223	the antimicrobial peptide lasioglossin	1186:1223	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	0	36	dep	Nanoparticles	58:70	arg1	Influence					91:99	Influence	91:99	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.	0:152	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.					
36499070	2	37	theme	drug	343:346	arg1	system					357:362	a magnetically controlled drug delivery system	317:362	a magnetically controlled drug delivery system	317:362	To develop a magnetically controlled drug delivery system, many factors must be considered, including the composition, surface properties, size and agglomeration, magnetization, cytocompatibility, and drug activity.					
36499070	1	38	theme	great	228:232	arg1	interest					234:241	great interest	228:241	great interest in nanomedicine	228:257	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs) are of great interest in nanomedicine, especially for applications in drug delivery.					
36499070	8	39	theme	agglomeration	1093:1105	arg1	impact					1083:1088	the significant impact	1067:1088	the significant impact of agglomeration on the magnetophoretic behavior	1067:1137	New insights into the significant impact of agglomeration on the magnetophoretic behavior are shown.					
36499070	4	40	theme	ION	612:614	arg1	CMD					616:618	ION@CMD	612:618	ION@CMD	612:618	ION@CMD are synthesized by co-precipitation.					
36499070	10	41	theme	antimicrobial	1493:1505	arg1	activity					1507:1514	high antimicrobial activity	1488:1514	high antimicrobial activity	1488:1514	The systems showed high antimicrobial activity with a minimal inhibitory concentration of 1.13 µM (adsorption) and 1.70 µM (covalent).					
36499070	9	42	theme	drug	1161:1164	arg1	loadings					1166:1173	Remarkable drug loadings	1150:1173	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin	1150:1223	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	9	42	theme	drug	1161:1164	arg1	%					1178:1178	62%	1176:1178	62%	1176:1178	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	2	43	theme	many	365:368	arg1	factors					370:376	many factors	365:376	many factors	365:376	To develop a magnetically controlled drug delivery system, many factors must be considered, including the composition, surface properties, size and agglomeration, magnetization, cytocompatibility, and drug activity.					
36499070	7	44	theme	&lt	896:898	arg1	diameters					886:894	hydrodynamic diameters	873:894	hydrodynamic diameters &lt; 100 nm and a good cell viability	873:932	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	7	44	theme	&lt	896:898	arg1	nm					905:906	&lt; 100 nm	896:906	&lt; 100 nm	896:906	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	10	45	theme	minimal	1523:1529	arg1	concentration					1542:1554	a minimal inhibitory concentration	1521:1554	a minimal inhibitory concentration of 1.13 µM (adsorption) and 1.70 µM (covalent)	1521:1601	The systems showed high antimicrobial activity with a minimal inhibitory concentration of 1.13 µM (adsorption) and 1.70 µM (covalent).					
36499070	7	46	theme	hydrodynamic	873:884	arg1	diameters					886:894	hydrodynamic diameters	873:894	hydrodynamic diameters &lt; 100 nm and a good cell viability	873:932	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	7	46	theme	hydrodynamic	873:884	arg1	viability					924:932	a good cell viability	912:932	a good cell viability	912:932	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	7	46	theme	hydrodynamic	873:884	arg1	nm					905:906	&lt; 100 nm	896:906	&lt; 100 nm	896:906	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	11	47	with	peptide	1655:1661	arg1	carrier					1701:1707	a magnetically controllable drug carrier	1668:1707	a magnetically controllable drug carrier	1668:1707	This system successfully combines an antimicrobial peptide with a magnetically controllable drug carrier.					
36499070	1	48	theme	iron	189:192	arg1	nanoparticles					200:212	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles	154:212	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs)	154:219	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs) are of great interest in nanomedicine, especially for applications in drug delivery.					
36499070	1	48	theme	iron	189:192	arg1	IONs					215:218	IONs	215:218	IONs	215:218	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs) are of great interest in nanomedicine, especially for applications in drug delivery.					
36499070	6	49	theme	g	798:798	arg1	L-1					800:802	12.5-25.0 g L-1	788:802	12.5-25.0 g L-1 of CMD	788:809	Above 12.5-25.0 g L-1 of CMD, the particles are colloidally stable.					
36499070	9	50	theme	adsorption	1415:1424	arg1	method					1426:1431	the adsorption method	1411:1431	the adsorption method (24% drug loading, 28% efficiency)	1411:1466	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	5	51	theme	thicker	693:699	arg1	coating					701:707	a thicker coating	691:707	a thicker coating	691:707	A higher quantity of CMD leads to a thicker coating and a reduced superparamagnetic core size with decreasing magnetization.					
36499070	9	52	theme	antimicrobial	1190:1202	arg1	lasioglossin					1212:1223	the antimicrobial peptide lasioglossin	1186:1223	the antimicrobial peptide lasioglossin	1186:1223	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	3	53	theme	coating	553:559	arg1	thickness					561:569	the CMD coating thickness	545:569	the CMD coating thickness	545:569	This study reveals how the CMD coating thickness can influence these particle properties.					
36499070	7	54	theme	delivery	1031:1038	arg1	systems					1040:1046	drug delivery systems	1026:1046	drug delivery systems	1026:1046	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	9	55	theme	%	1436:1436	arg1	loading					1443:1449	24% drug loading	1434:1449	24% drug loading	1434:1449	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	9	55	theme	%	1436:1436	arg1	efficiency					1456:1465	28% efficiency	1452:1465	28% efficiency	1452:1465	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	0	56	from	Influence	91:99	arg1	Properties					142:151	the Particle Properties	129:151	the Particle Properties	129:151	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.					
36499070	5	57	theme	reduced	715:721	arg1	size					746:749	a reduced superparamagnetic core size	713:749	a reduced superparamagnetic core size	713:749	A higher quantity of CMD leads to a thicker coating and a reduced superparamagnetic core size with decreasing magnetization.					
36499070	5	58	theme	CMD	678:680	arg1	quantity					666:673	A higher quantity	657:673	A higher quantity of CMD	657:680	A higher quantity of CMD leads to a thicker coating and a reduced superparamagnetic core size with decreasing magnetization.					
36499070	7	59	theme	most	993:996	arg1	characteristics					1007:1021	two of the most critical characteristics	982:1021	two of the most critical characteristics of drug delivery systems	982:1046	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	9	60	with	coupling	1287:1294	arg1	method					1385:1390	the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method	1301:1390	the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method	1301:1390	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	7	61	theme	critical	998:1005	arg1	characteristics					1007:1021	two of the most critical characteristics	982:1021	two of the most critical characteristics of drug delivery systems	982:1046	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	7	62	theme	of	986:987	arg1	characteristics					1007:1021	two of the most critical characteristics	982:1021	two of the most critical characteristics of drug delivery systems	982:1046	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	9	63	theme	28	1452:1453	arg1	%					1454:1454	%	1454:1454	%	1454:1454	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	11	64	theme	controllable	1683:1694	arg1	carrier					1701:1707	a magnetically controllable drug carrier	1668:1707	a magnetically controllable drug carrier	1668:1707	This system successfully combines an antimicrobial peptide with a magnetically controllable drug carrier.					
36499070	0	65	theme	Particle	133:140	arg1	Properties					142:151	the Particle Properties	129:151	the Particle Properties	129:151	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.					
36499070	0	66	theme	Thickness	116:124	arg1	Influence					91:99	Influence	91:99	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.	0:152	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.					
36499070	7	67	theme	smooth	950:955	arg1	cells					964:968	smooth muscle cells	950:968	smooth muscle cells	950:968	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	5	68	theme	core	741:744	arg1	size					746:749	a reduced superparamagnetic core size	713:749	a reduced superparamagnetic core size	713:749	A higher quantity of CMD leads to a thicker coating and a reduced superparamagnetic core size with decreasing magnetization.					
36499070	9	69	theme	covalent	1278:1285	arg1	coupling					1287:1294	covalent coupling	1278:1294	covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency)	1278:1466	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	10	70	theme	µM	1589:1590	arg1	concentration					1542:1554	a minimal inhibitory concentration	1521:1554	a minimal inhibitory concentration of 1.13 µM (adsorption) and 1.70 µM (covalent)	1521:1601	The systems showed high antimicrobial activity with a minimal inhibitory concentration of 1.13 µM (adsorption) and 1.70 µM (covalent).					
36499070	0	71	theme	Superparamagnetic	29:45	arg1	Nanoparticles					58:70	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles	0:70	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.	0:152	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.					
36499070	8	72	theme	New	1049:1051	arg1	insights					1053:1060	New insights	1049:1060	New insights into the significant impact of agglomeration on the magnetophoretic behavior	1049:1137	New insights into the significant impact of agglomeration on the magnetophoretic behavior are shown.					
36499070	0	73	theme	Oxide	52:56	arg1	Nanoparticles					58:70	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles	0:70	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.	0:152	Carboxymethyl-Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles for Drug Delivery: Influence of the Coating Thickness on the Particle Properties.					
36499070	1	74	theme	drug	291:294	arg1	delivery					296:303	drug delivery	291:303	drug delivery	291:303	Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs) are of great interest in nanomedicine, especially for applications in drug delivery.					
36499070	7	75	theme	good	914:917	arg1	diameters					886:894	hydrodynamic diameters	873:894	hydrodynamic diameters &lt; 100 nm and a good cell viability	873:932	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	7	75	theme	good	914:917	arg1	viability					924:932	a good cell viability	912:932	a good cell viability	912:932	All the particles show hydrodynamic diameters &lt; 100 nm and a good cell viability in contact with smooth muscle cells, fulfilling two of the most critical characteristics of drug delivery systems.					
36499070	8	76	theme	significant	1071:1081	arg1	impact					1083:1088	the significant impact	1067:1088	the significant impact of agglomeration on the magnetophoretic behavior	1067:1137	New insights into the significant impact of agglomeration on the magnetophoretic behavior are shown.					
36499070	9	77	dep	method	1426:1431	arg1	loading					1443:1449	24% drug loading	1434:1449	24% drug loading	1434:1449	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	9	77	dep	method	1426:1431	arg1	efficiency					1456:1465	28% efficiency	1452:1465	28% efficiency	1452:1465	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	2	78	theme	delivery	348:355	arg1	system					357:362	a magnetically controlled drug delivery system	317:362	a magnetically controlled drug delivery system	317:362	To develop a magnetically controlled drug delivery system, many factors must be considered, including the composition, surface properties, size and agglomeration, magnetization, cytocompatibility, and drug activity.					
36499070	4	79	theme	@	615:615	arg1	CMD					616:618	ION@CMD	612:618	ION@CMD	612:618	ION@CMD are synthesized by co-precipitation.					
36499070	11	80	theme	antimicrobial	1641:1653	arg1	peptide					1655:1661	an antimicrobial peptide	1638:1661	an antimicrobial peptide with a magnetically controllable drug carrier	1638:1707	This system successfully combines an antimicrobial peptide with a magnetically controllable drug carrier.					
36499070	2	81	theme	controlled	332:341	arg1	system					357:362	a magnetically controlled drug delivery system	317:362	a magnetically controlled drug delivery system	317:362	To develop a magnetically controlled drug delivery system, many factors must be considered, including the composition, surface properties, size and agglomeration, magnetization, cytocompatibility, and drug activity.					
36499070	8	82	theme	magnetophoretic	1114:1128	arg1	behavior					1130:1137	the magnetophoretic behavior	1110:1137	the magnetophoretic behavior	1110:1137	New insights into the significant impact of agglomeration on the magnetophoretic behavior are shown.					
36499070	10	83	theme	high	1488:1491	arg1	activity					1507:1514	high antimicrobial activity	1488:1514	high antimicrobial activity	1488:1514	The systems showed high antimicrobial activity with a minimal inhibitory concentration of 1.13 µM (adsorption) and 1.70 µM (covalent).					
36499070	10	84	dep	µM	1589:1590	arg1	covalent					1593:1600	covalent	1593:1600	covalent	1593:1600	The systems showed high antimicrobial activity with a minimal inhibitory concentration of 1.13 µM (adsorption) and 1.70 µM (covalent).					
36499070	9	85	theme	Remarkable	1150:1159	arg1	loadings					1166:1173	Remarkable drug loadings	1150:1173	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin	1150:1223	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	9	85	theme	Remarkable	1150:1159	arg1	%					1178:1178	62%	1176:1178	62%	1176:1178	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36499070	9	86	theme	carbodiimide/N-hydroxysuccinimide	1350:1382	arg1	N-ethyl-N'-					1314:1324	N-ethyl-N'-	1314:1324	N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide	1314:1382	Remarkable drug loadings (62%) with the antimicrobial peptide lasioglossin and an excellent efficiency (82.3%) were obtained by covalent coupling with the EDC/NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) method in comparison with the adsorption method (24% drug loading, 28% efficiency).					
36904200	7	0	theme	baby	1221:1224	arg1	products					1231:1238	baby food products	1221:1238	baby food products	1221:1238	Our results stated also that the contribution to the daily value for the saturated fatty acids, added sugars, and protein exceeded the daily recommended intake for most infant formulas and baby food products.					
36904200	0	1	dep	Fed	128:130	arg1	Call					175:178	A Call	173:178	A Call to Action	173:188	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	0	2	theme	Foods	82:86	arg1	Composition					18:28	the Composition	14:28	the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products	14:117	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	0	3	from	Children	152:159	arg1	Lebanon					164:170	Lebanon	164:170	Lebanon	164:170	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	0	4	theme	Commercial	93:102	arg1	Products					110:117	Commercial Snack Products	93:117	Commercial Snack Products	93:117	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	7	5	theme	added	1128:1132	arg1	sugars					1134:1139	added sugars	1128:1139	added sugars	1128:1139	Our results stated also that the contribution to the daily value for the saturated fatty acids, added sugars, and protein exceeded the daily recommended intake for most infant formulas and baby food products.					
36904200	2	6	theme	nutritional	490:500	arg1	content					502:508	their nutritional content	484:508	their nutritional content	484:508	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	4	7	theme	fatty	677:681	arg1	acids					683:687	all saturated fatty acids	663:687	all saturated fatty acids	663:687	Among all saturated fatty acids, palmitic acid (C16:0) accounted for the greatest proportion.					
36904200	5	8	theme	predominant	790:800	arg1	sugars					808:813	the predominant added sugars	786:813	the predominant added sugars in infant formulas	786:832	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	5	8	theme	predominant	790:800	arg1	sucrose					773:779	sucrose	773:779	sucrose	773:779	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	5	8	theme	predominant	790:800	arg1	glucose					761:767	glucose	761:767	glucose	761:767	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	1	9	theme	optimum	221:227	arg1	growth					229:234	optimum growth	221:234	optimum growth	221:234	(1) Background: Nutrition for optimum growth and physical development is acquired by adequate infant feeding practices.					
36904200	5	10	theme	added	802:806	arg1	sugars					808:813	the predominant added sugars	786:813	the predominant added sugars in infant formulas	786:832	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	5	10	theme	added	802:806	arg1	sucrose					773:779	sucrose	773:779	sucrose	773:779	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	5	10	theme	added	802:806	arg1	glucose					761:767	glucose	761:767	glucose	761:767	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	0	11	theme	Products	110:117	arg1	Composition					18:28	the Composition	14:28	the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products	14:117	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	7	12	theme	daily	1085:1089	arg1	value					1091:1095	the daily value	1081:1095	the daily value for the saturated fatty acids, added sugars, and protein	1081:1152	Our results stated also that the contribution to the daily value for the saturated fatty acids, added sugars, and protein exceeded the daily recommended intake for most infant formulas and baby food products.					
36904200	7	13	theme	fatty	1115:1119	arg1	acids					1121:1125	the saturated fatty acids	1101:1125	the saturated fatty acids	1101:1125	Our results stated also that the contribution to the daily value for the saturated fatty acids, added sugars, and protein exceeded the daily recommended intake for most infant formulas and baby food products.					
36904200	2	14	theme	different	346:354	arg1	brands					356:361	One hundred seventeen different brands	324:361	One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76)	324:421	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	0	15	theme	Snack	104:108	arg1	Products					110:117	Commercial Snack Products	93:117	Commercial Snack Products	93:117	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	3	16	theme	follow-up	587:595	arg1	formulas					597:604	follow-up formulas	587:604	follow-up formulas (79.85 g/100 g)	587:620	(3) Results: Saturated fatty acid content was detected to be the highest in follow-up formulas (79.85 g/100 g) and milky cereals (75.38 g/100 g).					
36904200	3	16	theme	follow-up	587:595	arg1	g					619:619	79.85 g/100 g	607:619	79.85 g/100 g	607:619	(3) Results: Saturated fatty acid content was detected to be the highest in follow-up formulas (79.85 g/100 g) and milky cereals (75.38 g/100 g).					
36904200	0	17	theme	Breastmilk	33:42	arg1	Composition					18:28	the Composition	14:28	the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products	14:117	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	2	18	dep	Methods	315:321	arg1	analyzed					471:478	analyzed	471:478	were analyzed for their nutritional content	466:508	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	2	18	dep	Methods	315:321	arg1	2					312:312	2	312:312	2	312:312	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	2	18	dep	Methods	315:321	arg1	selected					428:435	selected	428:435	were selected from the Lebanese market	423:460	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	3	19	theme	milky	626:630	arg1	cereals					632:638	milky cereals	626:638	milky cereals (75.38 g/100 g)	626:654	(3) Results: Saturated fatty acid content was detected to be the highest in follow-up formulas (79.85 g/100 g) and milky cereals (75.38 g/100 g).					
36904200	3	19	theme	milky	626:630	arg1	g					653:653	75.38 g/100 g	641:653	75.38 g/100 g	641:653	(3) Results: Saturated fatty acid content was detected to be the highest in follow-up formulas (79.85 g/100 g) and milky cereals (75.38 g/100 g).					
36904200	8	20	from	policymakers	1296:1307	arg1	evaluation					1280:1289	careful evaluation	1272:1289	careful evaluation from policymakers in order to improve the infant and young children feeding practices	1272:1375	(4) Conclusions: This requires careful evaluation from policymakers in order to improve the infant and young children feeding practices.					
36904200	2	21	theme	products	405:412	arg1	brands					356:361	One hundred seventeen different brands	324:361	One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76)	324:421	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	2	22	theme	=	417:417	arg1	n					415:415	n = 76	415:420	n = 76	415:420	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	2	22	theme	=	417:417	arg1	products					405:412	baby food products	395:412	baby food products (n = 76)	395:421	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	7	23	theme	infant	1201:1206	arg1	formulas					1208:1215	most infant formulas	1196:1215	most infant formulas	1196:1215	Our results stated also that the contribution to the daily value for the saturated fatty acids, added sugars, and protein exceeded the daily recommended intake for most infant formulas and baby food products.					
36904200	2	24	theme	=	385:385	arg1	n					383:383	n = 41	383:388	n = 41	383:388	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	2	24	theme	=	385:385	arg1	formulas					373:380	infant formulas	366:380	infant formulas (n = 41)	366:389	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	7	25	theme	food	1226:1229	arg1	products					1231:1238	baby food products	1221:1238	baby food products	1221:1238	Our results stated also that the contribution to the daily value for the saturated fatty acids, added sugars, and protein exceeded the daily recommended intake for most infant formulas and baby food products.					
36904200	4	26	theme	palmitic	690:697	arg1	C16:0					705:709	C16:0	705:709	C16:0	705:709	Among all saturated fatty acids, palmitic acid (C16:0) accounted for the greatest proportion.					
36904200	4	26	theme	palmitic	690:697	arg1	acid					699:702	palmitic acid	690:702	palmitic acid (C16:0)	690:710	Among all saturated fatty acids, palmitic acid (C16:0) accounted for the greatest proportion.					
36904200	3	27	theme	fatty	534:538	arg1	acid					540:543	Saturated fatty acid	524:543	Saturated fatty acid content	524:551	(3) Results: Saturated fatty acid content was detected to be the highest in follow-up formulas (79.85 g/100 g) and milky cereals (75.38 g/100 g).					
36904200	0	28	theme	Composition	18:28	arg1	Assessment					0:9	Assessment	0:9	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products	0:117	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	1	29	theme	physical	240:247	arg1	development					249:259	physical development	240:259	physical development	240:259	(1) Background: Nutrition for optimum growth and physical development is acquired by adequate infant feeding practices.					
36904200	5	30	theme	main	857:860	arg1	sugar					868:872	the main added sugar	853:872	the main added sugar in baby food products	853:894	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	5	30	theme	main	857:860	arg1	sucrose					841:847	sucrose	841:847	sucrose	841:847	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	5	31	from	sugar	868:872	arg1	products					887:894	baby food products	877:894	baby food products	877:894	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	2	32	theme	formulas	373:380	arg1	brands					356:361	One hundred seventeen different brands	324:361	One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76)	324:421	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	8	33	theme	young	1344:1348	arg1	practices					1367:1375	the infant and young children feeding practices	1329:1375	the infant and young children feeding practices	1329:1375	(4) Conclusions: This requires careful evaluation from policymakers in order to improve the infant and young children feeding practices.					
36904200	2	34	theme	Lebanese	446:453	arg1	market					455:460	the Lebanese market	442:460	the Lebanese market	442:460	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	1	35	dep	Background	195:204	arg1	1					192:192	1	192:192	1	192:192	(1) Background: Nutrition for optimum growth and physical development is acquired by adequate infant feeding practices.					
36904200	1	35	dep	Background	195:204	arg1	acquired					264:271	acquired	264:271	is acquired by adequate infant feeding practices	261:308	(1) Background: Nutrition for optimum growth and physical development is acquired by adequate infant feeding practices.					
36904200	7	36	theme	most	1196:1199	arg1	formulas					1208:1215	most infant formulas	1196:1215	most infant formulas	1196:1215	Our results stated also that the contribution to the daily value for the saturated fatty acids, added sugars, and protein exceeded the daily recommended intake for most infant formulas and baby food products.					
36904200	2	37	theme	infant	366:371	arg1	n					383:383	n = 41	383:388	n = 41	383:388	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	2	37	theme	infant	366:371	arg1	formulas					373:380	infant formulas	366:380	infant formulas (n = 41)	366:389	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	2	38	theme	food	400:403	arg1	n					415:415	n = 76	415:420	n = 76	415:420	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	2	38	theme	food	400:403	arg1	products					405:412	baby food products	395:412	baby food products (n = 76)	395:421	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	8	39	dep	Conclusions	1245:1255	arg1	4					1242:1242	4	1242:1242	4	1242:1242	(4) Conclusions: This requires careful evaluation from policymakers in order to improve the infant and young children feeding practices.					
36904200	3	40	theme	acid	540:543	arg1	highest					576:582	highest	576:582	highest	576:582	(3) Results: Saturated fatty acid content was detected to be the highest in follow-up formulas (79.85 g/100 g) and milky cereals (75.38 g/100 g).					
36904200	3	40	theme	acid	540:543	arg1	content					545:551	Saturated fatty acid content	524:551	Saturated fatty acid content	524:551	(3) Results: Saturated fatty acid content was detected to be the highest in follow-up formulas (79.85 g/100 g) and milky cereals (75.38 g/100 g).					
36904200	7	41	theme	recommended	1173:1183	arg1	intake					1185:1190	the daily recommended intake	1163:1190	the daily recommended intake for most infant formulas and baby food products	1163:1238	Our results stated also that the contribution to the daily value for the saturated fatty acids, added sugars, and protein exceeded the daily recommended intake for most infant formulas and baby food products.					
36904200	2	42	theme	baby	395:398	arg1	n					415:415	n = 76	415:420	n = 76	415:420	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	2	42	theme	baby	395:398	arg1	products					405:412	baby food products	395:412	baby food products (n = 76)	395:421	(2) Methods: One hundred seventeen different brands of infant formulas (n = 41) and baby food products (n = 76) were selected from the Lebanese market and were analyzed for their nutritional content.					
36904200	8	43	theme	feeding	1359:1365	arg1	practices					1367:1375	the infant and young children feeding practices	1329:1375	the infant and young children feeding practices	1329:1375	(4) Conclusions: This requires careful evaluation from policymakers in order to improve the infant and young children feeding practices.					
36904200	7	44	theme	daily	1167:1171	arg1	intake					1185:1190	the daily recommended intake	1163:1190	the daily recommended intake for most infant formulas and baby food products	1163:1238	Our results stated also that the contribution to the daily value for the saturated fatty acids, added sugars, and protein exceeded the daily recommended intake for most infant formulas and baby food products.					
36904200	3	45	dep	Results	515:521	arg1	detected					557:564	detected	557:564	was detected to be the highest in follow-up formulas (79.85 g/100 g) and milky cereals (75.38 g/100 g)	553:654	(3) Results: Saturated fatty acid content was detected to be the highest in follow-up formulas (79.85 g/100 g) and milky cereals (75.38 g/100 g).					
36904200	5	46	theme	food	882:885	arg1	products					887:894	baby food products	877:894	baby food products	877:894	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	8	47	theme	children	1350:1357	arg1	practices					1367:1375	the infant and young children feeding practices	1329:1375	the infant and young children feeding practices	1329:1375	(4) Conclusions: This requires careful evaluation from policymakers in order to improve the infant and young children feeding practices.					
36904200	0	48	theme	Young	146:150	arg1	Children					152:159	Young Children	146:159	Young Children	146:159	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	3	49	theme	Saturated	524:532	arg1	acid					540:543	Saturated fatty acid	524:543	Saturated fatty acid content	524:551	(3) Results: Saturated fatty acid content was detected to be the highest in follow-up formulas (79.85 g/100 g) and milky cereals (75.38 g/100 g).					
36904200	0	50	dep	Breastmilk	33:42	arg1	Substitutes					44:54	Substitutes	44:54	Substitutes	44:54	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	6	51	theme	products	938:945	arg1	non-compliant					952:964	non-compliant	952:964	non-compliant	952:964	Our data showed that the majority of the products were non-compliant to the regulations and the manufacturers' nutrition facts labels.					
36904200	6	51	theme	products	938:945	arg1	majority					922:929	the majority	918:929	the majority of the products	918:945	Our data showed that the majority of the products were non-compliant to the regulations and the manufacturers' nutrition facts labels.					
36904200	1	52	theme	adequate	276:283	arg1	practices					300:308	adequate infant feeding practices	276:308	adequate infant feeding practices	276:308	(1) Background: Nutrition for optimum growth and physical development is acquired by adequate infant feeding practices.					
36904200	5	53	theme	added	862:866	arg1	sugar					868:872	the main added sugar	853:872	the main added sugar in baby food products	853:894	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	5	53	theme	added	862:866	arg1	sucrose					841:847	sucrose	841:847	sucrose	841:847	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	4	54	theme	saturated	667:675	arg1	acids					683:687	all saturated fatty acids	663:687	all saturated fatty acids	663:687	Among all saturated fatty acids, palmitic acid (C16:0) accounted for the greatest proportion.					
36904200	1	55	theme	infant	285:290	arg1	practices					300:308	adequate infant feeding practices	276:308	adequate infant feeding practices	276:308	(1) Background: Nutrition for optimum growth and physical development is acquired by adequate infant feeding practices.					
36904200	8	56	theme	infant	1333:1338	arg1	practices					1367:1375	the infant and young children feeding practices	1329:1375	the infant and young children feeding practices	1329:1375	(4) Conclusions: This requires careful evaluation from policymakers in order to improve the infant and young children feeding practices.					
36904200	5	57	theme	infant	818:823	arg1	formulas					825:832	infant formulas	818:832	infant formulas	818:832	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	0	58	from	Infant	135:140	arg1	Lebanon					164:170	Lebanon	164:170	Lebanon	164:170	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	4	59	theme	greatest	730:737	arg1	proportion					739:748	the greatest proportion	726:748	the greatest proportion	726:748	Among all saturated fatty acids, palmitic acid (C16:0) accounted for the greatest proportion.					
36904200	6	60	theme	facts	1018:1022	arg1	labels					1024:1029	the manufacturers' nutrition facts labels	989:1029	the manufacturers' nutrition facts labels	989:1029	Our data showed that the majority of the products were non-compliant to the regulations and the manufacturers' nutrition facts labels.					
36904200	8	61	theme	careful	1272:1278	arg1	evaluation					1280:1289	careful evaluation	1272:1289	careful evaluation from policymakers in order to improve the infant and young children feeding practices	1272:1375	(4) Conclusions: This requires careful evaluation from policymakers in order to improve the infant and young children feeding practices.					
36904200	0	62	theme	Complementary	68:80	arg1	Foods					82:86	Commercial Complementary Foods	57:86	Commercial Complementary Foods	57:86	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	5	63	theme	baby	877:880	arg1	products					887:894	baby food products	877:894	baby food products	877:894	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36904200	6	64	theme	nutrition	1008:1016	arg1	labels					1024:1029	the manufacturers' nutrition facts labels	989:1029	the manufacturers' nutrition facts labels	989:1029	Our data showed that the majority of the products were non-compliant to the regulations and the manufacturers' nutrition facts labels.					
36904200	7	65	theme	saturated	1105:1113	arg1	acids					1121:1125	the saturated fatty acids	1101:1125	the saturated fatty acids	1101:1125	Our results stated also that the contribution to the daily value for the saturated fatty acids, added sugars, and protein exceeded the daily recommended intake for most infant formulas and baby food products.					
36904200	0	66	theme	Commercial	57:66	arg1	Foods					82:86	Commercial Complementary Foods	57:86	Commercial Complementary Foods	57:86	Assessment of the Composition of Breastmilk Substitutes, Commercial Complementary Foods, and Commercial Snack Products Commonly Fed to Infant and Young Children in Lebanon: A Call to Action.					
36904200	1	67	theme	feeding	292:298	arg1	practices					300:308	adequate infant feeding practices	276:308	adequate infant feeding practices	276:308	(1) Background: Nutrition for optimum growth and physical development is acquired by adequate infant feeding practices.					
36904200	5	68	from	sugars	808:813	arg1	formulas					825:832	infant formulas	818:832	infant formulas	818:832	Moreover, glucose and sucrose were the predominant added sugars in infant formulas, while sucrose was the main added sugar in baby food products.					
36801216	2	0	theme	metal	589:593	arg1	MnFe					602:605	dual metal oxides MnFe	584:605	dual metal oxides MnFe	584:605	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	5	1	theme	high	1122:1125	arg1	efficiency					1127:1136	high efficiency	1122:1136	high efficiency	1122:1136	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	4	2	theme	broad	1061:1065	arg1	range					1070:1074	a broad pH range	1059:1074	a broad pH range (5.0-10.0)	1059:1085	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	4	2	theme	broad	1061:1065	arg1	5.0-10.0					1077:1084	5.0-10.0	1077:1084	5.0-10.0	1077:1084	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	6	3	theme	quenching	1473:1481	arg1	experiments					1483:1493	the free radical quenching experiments	1456:1493	the free radical quenching experiments	1456:1493	An AFB1 decontamination pathway proposed was based on the free radical quenching experiments and analysis of the degradation intermediates.					
36801216	4	4	theme	PMS/MF	920:925	arg1	system					933:938	PMS/MF@CRHHT system	920:938	PMS/MF@CRHHT system	920:938	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	0	5	theme	synergistic	206:216	arg1	mechanism					218:226	synergistic mechanism	206:226	synergistic mechanism	206:226	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	5	6	from	bond	1271:1274	arg1	transfer					1313:1320	then mutual electron transfer	1292:1320	then mutual electron transfer between them to enhanced electron density	1292:1362	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	5	6	from	bond	1271:1274	arg1	CRHHT					1282:1286	MF@CRHHT	1279:1286	MF@CRHHT	1279:1286	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	0	7	theme	husk	84:87	arg1	hercynite					117:125	chitosan/rice husk waste/iron aluminate spinel hercynite	70:125	chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1	70:176	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	4	8	theme	AFB1	904:907	arg1	removal					909:915	The AFB1 removal	900:915	The AFB1 removal in PMS/MF@CRHHT system	900:938	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	7	9	theme	biomass-based	1664:1676	arg1	activator					1678:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator	1579:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution	1579:1710	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	7	9	theme	biomass-based	1664:1676	arg1	CRHHT					1555:1559	the MF@CRHHT	1548:1559	the MF@CRHHT	1548:1559	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	2	10	theme	MF	564:565	arg1	CRHHT					567:571	MF@CRHHT	564:571	MF@CRHHT	564:571	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	10	theme	MF	564:565	arg1	biocomposite					550:561	a novel superparamagnetic MnFe biocomposite	519:561	a novel superparamagnetic MnFe biocomposite (MF@CRHHT)	519:572	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	0	11	theme	catalytic	137:145	arg1	detoxification					147:160	rapid catalytic detoxification	131:160	rapid catalytic detoxification of aflatoxin B1	131:176	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	1	12	theme	food	391:394	arg1	supply					396:401	safe food supply	386:401	safe food supply	386:401	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.					
36801216	2	13	theme	biocomposite	550:561	arg1	construction					503:514	the construction	499:514	the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way	499:801	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	14	theme	non-thermal/microbial	777:797	arg1	way					799:801	a non-thermal/microbial way	775:801	a non-thermal/microbial way	775:801	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	7	15	theme	environment-friendly	1622:1641	arg1	activator					1678:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator	1579:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution	1579:1710	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	7	15	theme	environment-friendly	1622:1641	arg1	CRHHT					1555:1559	the MF@CRHHT	1548:1559	the MF@CRHHT	1548:1559	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	4	16	theme	CRHHT	927:931	arg1	system					933:938	PMS/MF@CRHHT system	920:938	PMS/MF@CRHHT system	920:938	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	0	17	theme	B1	175:176	arg1	detoxification					147:160	rapid catalytic detoxification	131:160	rapid catalytic detoxification of aflatoxin B1	131:176	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	1	18	theme	human	408:412	arg1	health					414:419	human health	408:419	human health	408:419	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.					
36801216	7	19	theme	recoverable	1609:1619	arg1	activator					1678:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator	1579:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution	1579:1710	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	7	19	theme	recoverable	1609:1619	arg1	CRHHT					1555:1559	the MF@CRHHT	1548:1559	the MF@CRHHT	1548:1559	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	3	20	theme	spectroscopic	876:888	arg1	analyses					890:897	various spectroscopic analyses	868:897	various spectroscopic analyses	868:897	Structure, and morphology were comprehensively characterized by various spectroscopic analyses.					
36801216	7	21	theme	cost-effective	1593:1606	arg1	activator					1678:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator	1579:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution	1579:1710	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	7	21	theme	cost-effective	1593:1606	arg1	CRHHT					1555:1559	the MF@CRHHT	1548:1559	the MF@CRHHT	1548:1559	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	2	22	theme	AFB1	738:741	arg1	detoxification					743:756	rapid AFB1 detoxification	732:756	rapid AFB1 detoxification	732:756	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	5	23	theme	mutual	1297:1302	arg1	transfer					1313:1320	then mutual electron transfer	1292:1320	then mutual electron transfer between them to enhanced electron density	1292:1362	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	0	24	theme	chitosan/rice	70:82	arg1	hercynite					117:125	chitosan/rice husk waste/iron aluminate spinel hercynite	70:125	chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1	70:176	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	7	25	theme	efficient	1582:1590	arg1	activator					1678:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator	1579:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution	1579:1710	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	7	25	theme	efficient	1582:1590	arg1	CRHHT					1555:1559	the MF@CRHHT	1548:1559	the MF@CRHHT	1548:1559	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	0	26	theme	waste/iron	89:98	arg1	hercynite					117:125	chitosan/rice husk waste/iron aluminate spinel hercynite	70:125	chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1	70:176	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	5	27	theme	physical-chemical	1142:1158	arg1	properties					1160:1169	physical-chemical properties	1142:1169	physical-chemical properties	1142:1169	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	4	28	theme	excellent	992:1000	arg1	efficiency					1002:1011	excellent efficiency	992:1011	excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min)	992:1052	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	5	29	theme	@	1281:1281	arg1	CRHHT					1282:1286	MF@CRHHT	1279:1286	MF@CRHHT	1279:1286	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	0	30	theme	spinel	110:115	arg1	hercynite					117:125	chitosan/rice husk waste/iron aluminate spinel hercynite	70:125	chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1	70:176	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	2	31	theme	in/on	621:625	arg1	residues					649:656	in/on agricultural/forestry residues	621:656	in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles)	621:714	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	31	theme	in/on	621:625	arg1	nanoparticles					701:713	chitosan/rice husk waste/hercynite hybrid nanoparticles	659:713	chitosan/rice husk waste/hercynite hybrid nanoparticles	659:713	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	5	32	theme	enhanced	1338:1345	arg1	density					1356:1362	enhanced electron density	1338:1362	enhanced electron density	1338:1362	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	2	33	theme	waste/hercynite	678:692	arg1	residues					649:656	in/on agricultural/forestry residues	621:656	in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles)	621:714	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	33	theme	waste/hercynite	678:692	arg1	nanoparticles					701:713	chitosan/rice husk waste/hercynite hybrid nanoparticles	659:713	chitosan/rice husk waste/hercynite hybrid nanoparticles	659:713	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	0	34	theme	Superparamagnetic	0:16	arg1	composite					29:37	Superparamagnetic MnFe alloy composite	0:37	Superparamagnetic MnFe alloy composite	0:37	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	1	35	theme	carcinogen/mutagens	290:308	arg1	toxin					310:314	carcinogen/mutagens toxin	290:314	carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health	290:419	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.					
36801216	7	36	theme	remediate	1692:1700	arg1	pollution					1702:1710	remediate pollution	1692:1710	remediate pollution	1692:1710	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	2	37	theme	chitosan/rice	659:671	arg1	residues					649:656	in/on agricultural/forestry residues	621:656	in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles)	621:714	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	37	theme	chitosan/rice	659:671	arg1	nanoparticles					701:713	chitosan/rice husk waste/hercynite hybrid nanoparticles	659:713	chitosan/rice husk waste/hercynite hybrid nanoparticles	659:713	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	0	38	theme	alloy	23:27	arg1	composite					29:37	Superparamagnetic MnFe alloy composite	0:37	Superparamagnetic MnFe alloy composite	0:37	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	7	39	theme	@	1554:1554	arg1	activator					1678:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator	1579:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution	1579:1710	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	7	39	theme	@	1554:1554	arg1	CRHHT					1555:1559	the MF@CRHHT	1548:1559	the MF@CRHHT	1548:1559	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	2	40	theme	co-participation	467:482	arg1	strategies					484:493	co-participation strategies	467:493	co-participation strategies	467:493	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	1	41	theme	Aspergillus	328:338	arg1	threat					363:368	a major threat	355:368	a major threat to the economy, safe food supply, and human health	355:419	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.					
36801216	1	41	theme	Aspergillus	328:338	arg1	fungi					340:344	Aspergillus fungi	328:344	Aspergillus fungi that are a major threat to the economy, safe food supply, and human health	328:419	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.					
36801216	6	42	theme	decontamination	1410:1424	arg1	pathway					1426:1432	An AFB1 decontamination pathway	1402:1432	An AFB1 decontamination pathway proposed	1402:1441	An AFB1 decontamination pathway proposed was based on the free radical quenching experiments and analysis of the degradation intermediates.					
36801216	5	43	theme	mechanistic	1176:1186	arg1	insight					1188:1194	mechanistic insight	1176:1194	mechanistic insight	1176:1194	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	0	44	dep	derived	39:45	arg1	performance					190:200	performance	190:200	performance	190:200	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	0	44	dep	derived	39:45	arg1	mechanism					218:226	synergistic mechanism	206:226	synergistic mechanism	206:226	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	0	44	dep	derived	39:45	arg1	Structure					179:187	Structure	179:187	Structure	179:187	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	1	45	theme	foodstuffs	250:259	arg1	contamination					233:245	The contamination	229:245	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.	229:420	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.					
36801216	5	46	theme	formation	1256:1264	arg1	bond					1271:1274	the formation MnFe bond	1252:1274	the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density	1252:1362	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	1	47	with	foodstuffs	250:259	arg1	aflatoxins					266:275	aflatoxins B1 (AFB1)	266:285	aflatoxins B1 (AFB1)	266:285	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.					
36801216	4	48	theme	pH	1067:1068	arg1	range					1070:1074	a broad pH range	1059:1074	a broad pH range (5.0-10.0)	1059:1085	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	4	48	theme	pH	1067:1068	arg1	5.0-10.0					1077:1084	5.0-10.0	1077:1084	5.0-10.0	1077:1084	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	5	49	theme	oxygen	1386:1391	arg1	species					1393:1399	reactive oxygen species	1377:1399	reactive oxygen species	1377:1399	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	2	50	theme	dual	584:587	arg1	MnFe					602:605	dual metal oxides MnFe	584:605	dual metal oxides MnFe	584:605	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	6	51	theme	radical	1465:1471	arg1	experiments					1483:1493	the free radical quenching experiments	1456:1493	the free radical quenching experiments	1456:1493	An AFB1 decontamination pathway proposed was based on the free radical quenching experiments and analysis of the degradation intermediates.					
36801216	4	52	from	removal	909:915	arg1	system					933:938	PMS/MF@CRHHT system	920:938	PMS/MF@CRHHT system	920:938	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	7	53	theme	efficient	1654:1662	arg1	activator					1678:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator	1579:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution	1579:1710	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	7	53	theme	efficient	1654:1662	arg1	CRHHT					1555:1559	the MF@CRHHT	1548:1559	the MF@CRHHT	1548:1559	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	2	54	theme	oxides	595:600	arg1	MnFe					602:605	dual metal oxides MnFe	584:605	dual metal oxides MnFe	584:605	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	4	55	theme	pseudo-first-order	949:966	arg1	kinetics					968:975	pseudo-first-order kinetics	949:975	pseudo-first-order kinetics	949:975	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	1	56	dep	aflatoxins	266:275	arg1	AFB1					281:284	AFB1	281:284	AFB1	281:284	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.					
36801216	1	56	dep	aflatoxins	266:275	arg1	B1					277:278	B1	277:278	aflatoxins B1 (AFB1)	266:285	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.					
36801216	0	57	theme	aflatoxin	165:173	arg1	B1					175:176	aflatoxin B1	165:176	aflatoxin B1	165:176	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	6	58	theme	intermediates	1527:1539	arg1	experiments					1483:1493	the free radical quenching experiments	1456:1493	the free radical quenching experiments	1456:1493	An AFB1 decontamination pathway proposed was based on the free radical quenching experiments and analysis of the degradation intermediates.					
36801216	6	58	theme	intermediates	1527:1539	arg1	analysis					1499:1506	analysis	1499:1506	analysis of the degradation intermediates	1499:1539	An AFB1 decontamination pathway proposed was based on the free radical quenching experiments and analysis of the degradation intermediates.					
36801216	2	59	theme	MnFe	545:548	arg1	CRHHT					567:571	MF@CRHHT	564:571	MF@CRHHT	564:571	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	59	theme	MnFe	545:548	arg1	biocomposite					550:561	a novel superparamagnetic MnFe biocomposite	519:561	a novel superparamagnetic MnFe biocomposite (MF@CRHHT)	519:572	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	60	theme	superparamagnetic	527:543	arg1	CRHHT					567:571	MF@CRHHT	564:571	MF@CRHHT	564:571	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	60	theme	superparamagnetic	527:543	arg1	biocomposite					550:561	a novel superparamagnetic MnFe biocomposite	519:561	a novel superparamagnetic MnFe biocomposite (MF@CRHHT)	519:572	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	4	61	theme	@	926:926	arg1	system					933:938	PMS/MF@CRHHT system	920:938	PMS/MF@CRHHT system	920:938	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	2	62	theme	@	566:566	arg1	CRHHT					567:571	MF@CRHHT	564:571	MF@CRHHT	564:571	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	62	theme	@	566:566	arg1	biocomposite					550:561	a novel superparamagnetic MnFe biocomposite	519:561	a novel superparamagnetic MnFe biocomposite (MF@CRHHT)	519:572	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	6	63	theme	AFB1	1405:1408	arg1	pathway					1426:1432	An AFB1 decontamination pathway	1402:1432	An AFB1 decontamination pathway proposed	1402:1441	An AFB1 decontamination pathway proposed was based on the free radical quenching experiments and analysis of the degradation intermediates.					
36801216	2	64	theme	rapid	732:736	arg1	detoxification					743:756	rapid AFB1 detoxification	732:756	rapid AFB1 detoxification	732:756	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	0	65	theme	aluminate	100:108	arg1	hercynite					117:125	chitosan/rice husk waste/iron aluminate spinel hercynite	70:125	chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1	70:176	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	1	66	theme	major	357:361	arg1	threat					363:368	a major threat	355:368	a major threat to the economy, safe food supply, and human health	355:419	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.					
36801216	1	66	theme	major	357:361	arg1	fungi					340:344	Aspergillus fungi	328:344	Aspergillus fungi that are a major threat to the economy, safe food supply, and human health	328:419	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.					
36801216	4	67	dep	efficiency	1002:1011	arg1	%					1040:1040	83.1 %	1035:1040	83.1 % in 5.0 min	1035:1051	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	4	67	dep	efficiency	1002:1011	arg1	%					1019:1019	99.3 %	1014:1019	99.3 % in 20 min	1014:1029	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	5	68	theme	MF	1279:1280	arg1	CRHHT					1282:1286	MF@CRHHT	1279:1286	MF@CRHHT	1279:1286	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	3	69	theme	various	868:874	arg1	analyses					890:897	various spectroscopic analyses	868:897	various spectroscopic analyses	868:897	Structure, and morphology were comprehensively characterized by various spectroscopic analyses.					
36801216	0	70	theme	hercynite	117:125	arg1	cross-bindered					52:65	cross-bindered	52:65	cross-bindered	52:65	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	2	71	theme	novel	521:525	arg1	CRHHT					567:571	MF@CRHHT	564:571	MF@CRHHT	564:571	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	71	theme	novel	521:525	arg1	biocomposite					550:561	a novel superparamagnetic MnFe biocomposite	519:561	a novel superparamagnetic MnFe biocomposite (MF@CRHHT)	519:572	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	72	theme	agricultural/forestry	627:647	arg1	residues					649:656	in/on agricultural/forestry residues	621:656	in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles)	621:714	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	72	theme	agricultural/forestry	627:647	arg1	nanoparticles					701:713	chitosan/rice husk waste/hercynite hybrid nanoparticles	659:713	chitosan/rice husk waste/hercynite hybrid nanoparticles	659:713	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	0	73	theme	rapid	131:135	arg1	detoxification					147:160	rapid catalytic detoxification	131:160	rapid catalytic detoxification of aflatoxin B1	131:176	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	2	74	theme	hybrid	694:699	arg1	residues					649:656	in/on agricultural/forestry residues	621:656	in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles)	621:714	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	74	theme	hybrid	694:699	arg1	nanoparticles					701:713	chitosan/rice husk waste/hercynite hybrid nanoparticles	659:713	chitosan/rice husk waste/hercynite hybrid nanoparticles	659:713	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	5	75	theme	electron	1347:1354	arg1	density					1356:1362	enhanced electron density	1338:1362	enhanced electron density	1338:1362	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	0	76	theme	MnFe	18:21	arg1	composite					29:37	Superparamagnetic MnFe alloy composite	0:37	Superparamagnetic MnFe alloy composite	0:37	Superparamagnetic MnFe alloy composite derived from cross-bindered of chitosan/rice husk waste/iron aluminate spinel hercynite for rapid catalytic detoxification of aflatoxin B1: Structure, performance and synergistic mechanism.					
36801216	1	77	theme	safe	386:389	arg1	supply					396:401	safe food supply	386:401	safe food supply	386:401	The contamination of foodstuffs with aflatoxins B1 (AFB1) as carcinogen/mutagens toxin produced by Aspergillus fungi that are a major threat to the economy, safe food supply, and human health.					
36801216	2	78	theme	husk	673:676	arg1	residues					649:656	in/on agricultural/forestry residues	621:656	in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles)	621:714	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	2	78	theme	husk	673:676	arg1	nanoparticles					701:713	chitosan/rice husk waste/hercynite hybrid nanoparticles	659:713	chitosan/rice husk waste/hercynite hybrid nanoparticles	659:713	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	5	79	theme	electron	1304:1311	arg1	transfer					1313:1320	then mutual electron transfer	1292:1320	then mutual electron transfer between them to enhanced electron density	1292:1362	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	5	80	theme	synergistic	1213:1223	arg1	effect					1225:1230	the synergistic effect	1209:1230	the synergistic effect	1209:1230	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	5	80	theme	synergistic	1213:1223	arg1	related					1241:1247	related	1241:1247	related	1241:1247	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	4	81	from	%	1019:1019	arg1	5.0 min					1045:1051	5.0 min	1045:1051	5.0 min	1045:1051	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	4	81	from	%	1019:1019	arg1	20 min					1024:1029	20 min	1024:1029	20 min	1024:1029	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	4	82	from	%	1040:1040	arg1	5.0 min					1045:1051	5.0 min	1045:1051	5.0 min	1045:1051	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	4	82	from	%	1040:1040	arg1	20 min					1024:1029	20 min	1024:1029	20 min	1024:1029	The AFB1 removal in PMS/MF@CRHHT system followed pseudo-first-order kinetics, and exhibited excellent efficiency (99.3 % in 20 min and 83.1 % in 5.0 min) over a broad pH range (5.0-10.0).					
36801216	7	83	theme	MF	1552:1553	arg1	activator					1678:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator	1579:1686	an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution	1579:1710	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	7	83	theme	MF	1552:1553	arg1	CRHHT					1555:1559	the MF@CRHHT	1548:1559	the MF@CRHHT	1548:1559	Thus, the MF@CRHHT can be applied as an efficient, cost-effective, recoverable, environment-friendly and highly efficient biomass-based activator for remediate pollution.					
36801216	2	84	theme	facile	439:444	arg1	wet-impregnation					446:461	a facile wet-impregnation	437:461	a facile wet-impregnation	437:461	To, we present a facile wet-impregnation and co-participation strategies for the construction of a novel superparamagnetic MnFe biocomposite (MF@CRHHT), in which dual metal oxides MnFe were anchored in/on agricultural/forestry residues (chitosan/rice husk waste/hercynite hybrid nanoparticles) and applied for rapid AFB1 detoxification by destroying in a non-thermal/microbial way.					
36801216	6	85	theme	degradation	1515:1525	arg1	intermediates					1527:1539	the degradation intermediates	1511:1539	the degradation intermediates	1511:1539	An AFB1 decontamination pathway proposed was based on the free radical quenching experiments and analysis of the degradation intermediates.					
36801216	5	86	theme	reactive	1377:1384	arg1	species					1393:1399	reactive oxygen species	1377:1399	reactive oxygen species	1377:1399	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36801216	6	87	theme	free	1460:1463	arg1	experiments					1483:1493	the free radical quenching experiments	1456:1493	the free radical quenching experiments	1456:1493	An AFB1 decontamination pathway proposed was based on the free radical quenching experiments and analysis of the degradation intermediates.					
36801216	5	88	theme	MnFe	1266:1269	arg1	bond					1271:1274	the formation MnFe bond	1252:1274	the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density	1252:1362	Importantly, relationship between high efficiency and physical-chemical properties, and mechanistic insight reveals that the synergistic effect could be related to the formation MnFe bond in MF@CRHHT and then mutual electron transfer between them to enhanced electron density and generate reactive oxygen species.					
36781283	7	0	theme	high	1242:1245	arg1	sensitivity					1247:1257	a high sensitivity	1240:1257	a high sensitivity of 33 %·°C-1, which is promising to monitor the changes in temperature	1240:1328	Additionally, ACP gel could be served to detect temperature changes with a wide operating range and a high sensitivity of 33 %·°C-1, which is promising to monitor the changes in temperature.					
36781283	6	1	theme	cutting-healing	1062:1076	arg1	steps					1078:1082	multiple cutting-healing steps	1053:1082	multiple cutting-healing steps	1053:1082	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	6	2	theme	ACP	907:909	arg1	gel					911:913	ACP gel	907:913	ACP gel	907:913	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	3	3	theme	gel	569:571	arg1	structure					499:507	a multiple network structure	480:507	a multiple network structure of amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel	480:571	Based on that, a multiple network structure of amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel was prepared by a "cooking" method.					
36781283	1	4	theme	recent	266:271	arg1	years					273:277	recent years	266:277	recent years	266:277	Starch with active hydroxyl groups is one of the most attractive carbohydrates for the preparation of gels in recent years.					
36781283	0	5	theme	starch-based	92:103	arg1	gel					105:107	High-stretchable, self-healing, self-adhesive, self-extinguishing, low-temperature tolerant starch-based gel	0:107	High-stretchable, self-healing, self-adhesive, self-extinguishing, low-temperature tolerant starch-based gel	0:107	High-stretchable, self-healing, self-adhesive, self-extinguishing, low-temperature tolerant starch-based gel and its application in stimuli-responsiveness.					
36781283	5	6	from	behavior	803:810	arg1	elongation					722:731	high elongation	717:731	high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing	717:884	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	9	7	theme	multiple	1624:1631	arg1	performance					1633:1643	multiple performance	1624:1643	multiple performance	1624:1643	This study provides new opportunities for the design and fabrication of carbohydrate-based gels with multiple performance and multifunctional electronic devices.					
36781283	2	8	theme	low-temperature	378:392	arg1	resistance					394:403	low-temperature resistance	378:403	especially low-temperature resistance	367:403	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	2	8	theme	low-temperature	378:392	arg1	properties					304:313	the mechanical properties	289:313	the mechanical properties	289:313	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	5	9	from	strength	755:762	arg1	elongation					722:731	high elongation	717:731	high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing	717:884	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	8	10	theme	cooking	1380:1386	arg1	process					1388:1394	the cooking process	1376:1394	the cooking process	1376:1394	More interestingly, ACP gel can even monitor the cooking process and breathing frequency with fast response, implying applications in food processing, disease diagnosis and medical treatment.					
36781283	9	11	theme	electronic	1665:1674	arg1	devices					1676:1682	multifunctional electronic devices	1649:1682	multifunctional electronic devices	1649:1682	This study provides new opportunities for the design and fabrication of carbohydrate-based gels with multiple performance and multifunctional electronic devices.					
36781283	5	12	theme	ACP	696:698	arg1	gel					700:702	ACP gel	696:702	ACP gel	696:702	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	3	13	theme	cooking	592:598	arg1	method					601:606	a "cooking" method	589:606	a "cooking" method	589:606	Based on that, a multiple network structure of amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel was prepared by a "cooking" method.					
36781283	6	14	theme	low-temperature	1091:1105	arg1	conditions					1107:1116	low-temperature conditions	1091:1116	low-temperature conditions	1091:1116	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	5	15	from	break	736:740	arg1	elongation					722:731	high elongation	717:731	high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing	717:884	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	4	16	theme	hydrogen	657:664	arg1	bonds					666:670	multiple hydrogen bonds	648:670	multiple hydrogen bonds	648:670	Tannic acid (TA) was used to construct multiple hydrogen bonds among molecular chains.					
36781283	2	17	theme	mechanical	293:302	arg1	resistance					394:403	low-temperature resistance	378:403	especially low-temperature resistance	367:403	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	2	17	theme	mechanical	293:302	arg1	unsatisfactory					419:432	unsatisfactory	419:432	unsatisfactory	419:432	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	2	17	theme	mechanical	293:302	arg1	properties					304:313	the mechanical properties	289:313	the mechanical properties	289:313	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	2	17	theme	mechanical	293:302	arg1	properties					329:338	self-healing properties	316:338	self-healing properties	316:338	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	2	17	theme	mechanical	293:302	arg1	properties					355:364	self-adhesion properties	341:364	self-adhesion properties	341:364	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	3	18	theme	"	599:599	arg1	method					601:606	a "cooking" method	589:606	a "cooking" method	589:606	Based on that, a multiple network structure of amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel was prepared by a "cooking" method.					
36781283	6	19	theme	harsh	1029:1033	arg1	conditions					1035:1044	harsh conditions	1029:1044	harsh conditions	1029:1044	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	7	20	theme	operating	1220:1228	arg1	range					1230:1234	a wide operating range	1213:1234	a wide operating range	1213:1234	Additionally, ACP gel could be served to detect temperature changes with a wide operating range and a high sensitivity of 33 %·°C-1, which is promising to monitor the changes in temperature.					
36781283	9	21	dep	design	1569:1574	arg1	the					1565:1567	the	1565:1567	the	1565:1567	This study provides new opportunities for the design and fabrication of carbohydrate-based gels with multiple performance and multifunctional electronic devices.					
36781283	1	22	with	Starch	156:161	arg1	groups					184:189	active hydroxyl groups	168:189	active hydroxyl groups	168:189	Starch with active hydroxyl groups is one of the most attractive carbohydrates for the preparation of gels in recent years.					
36781283	3	23	theme	amylopectin-carboxymethyl	512:536	arg1	gel					569:571	amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel	512:571	amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel	512:571	Based on that, a multiple network structure of amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel was prepared by a "cooking" method.					
36781283	9	24	theme	gels	1614:1617	arg1	design					1569:1574	design	1569:1574	design	1569:1574	This study provides new opportunities for the design and fabrication of carbohydrate-based gels with multiple performance and multifunctional electronic devices.					
36781283	9	24	theme	gels	1614:1617	arg1	fabrication					1580:1590	fabrication	1580:1590	fabrication	1580:1590	This study provides new opportunities for the design and fabrication of carbohydrate-based gels with multiple performance and multifunctional electronic devices.					
36781283	2	25	theme	starch-based	446:457	arg1	gels					459:462	current starch-based gels	438:462	current starch-based gels	438:462	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	5	26	from	resistance	843:852	arg1	elongation					722:731	high elongation	717:731	high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing	717:884	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	7	27	theme	wide	1215:1218	arg1	range					1230:1234	a wide operating range	1213:1234	a wide operating range	1213:1234	Additionally, ACP gel could be served to detect temperature changes with a wide operating range and a high sensitivity of 33 %·°C-1, which is promising to monitor the changes in temperature.					
36781283	2	28	theme	current	438:444	arg1	gels					459:462	current starch-based gels	438:462	current starch-based gels	438:462	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	9	29	theme	new	1543:1545	arg1	opportunities					1547:1559	new opportunities	1543:1559	new opportunities for the design and fabrication of carbohydrate-based gels with multiple performance and multifunctional electronic devices	1543:1682	This study provides new opportunities for the design and fabrication of carbohydrate-based gels with multiple performance and multifunctional electronic devices.					
36781283	5	30	theme	adhesion	794:801	arg1	behavior					803:810	adhesion behavior	794:810	adhesion behavior	794:810	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	0	31	theme	High-stretchable	0:15	arg1	gel					105:107	High-stretchable, self-healing, self-adhesive, self-extinguishing, low-temperature tolerant starch-based gel	0:107	High-stretchable, self-healing, self-adhesive, self-extinguishing, low-temperature tolerant starch-based gel	0:107	High-stretchable, self-healing, self-adhesive, self-extinguishing, low-temperature tolerant starch-based gel and its application in stimuli-responsiveness.					
36781283	8	32	theme	disease	1482:1488	arg1	diagnosis					1490:1498	disease diagnosis	1482:1498	disease diagnosis	1482:1498	More interestingly, ACP gel can even monitor the cooking process and breathing frequency with fast response, implying applications in food processing, disease diagnosis and medical treatment.					
36781283	2	33	theme	self-healing	316:327	arg1	properties					304:313	the mechanical properties	289:313	the mechanical properties	289:313	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	2	33	theme	self-healing	316:327	arg1	properties					329:338	self-healing properties	316:338	self-healing properties	316:338	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	1	34	theme	attractive	210:219	arg1	carbohydrates					221:233	the most attractive carbohydrates	201:233	the most attractive carbohydrates for the preparation of gels in recent years	201:277	Starch with active hydroxyl groups is one of the most attractive carbohydrates for the preparation of gels in recent years.					
36781283	6	35	theme	real-time	1002:1010	arg1	monitoring					1012:1021	real-time monitoring	1002:1021	real-time monitoring under harsh conditions	1002:1044	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	0	36	from	gel	105:107	arg1	stimuli-responsiveness					132:153	stimuli-responsiveness	132:153	stimuli-responsiveness	132:153	High-stretchable, self-healing, self-adhesive, self-extinguishing, low-temperature tolerant starch-based gel and its application in stimuli-responsiveness.					
36781283	7	37	theme	·°C-1	1266:1270	arg1	sensitivity					1247:1257	a high sensitivity	1240:1257	a high sensitivity of 33 %·°C-1, which is promising to monitor the changes in temperature	1240:1328	Additionally, ACP gel could be served to detect temperature changes with a wide operating range and a high sensitivity of 33 %·°C-1, which is promising to monitor the changes in temperature.					
36781283	7	37	theme	·°C-1	1266:1270	arg1	range					1230:1234	a wide operating range	1213:1234	a wide operating range	1213:1234	Additionally, ACP gel could be served to detect temperature changes with a wide operating range and a high sensitivity of 33 %·°C-1, which is promising to monitor the changes in temperature.					
36781283	1	38	theme	carbohydrates	221:233	arg1	carbohydrates					221:233	the most attractive carbohydrates	201:233	the most attractive carbohydrates for the preparation of gels in recent years	201:277	Starch with active hydroxyl groups is one of the most attractive carbohydrates for the preparation of gels in recent years.					
36781283	1	38	theme	carbohydrates	221:233	arg1	one					194:196	one	194:196	one	194:196	Starch with active hydroxyl groups is one of the most attractive carbohydrates for the preparation of gels in recent years.					
36781283	4	39	theme	Tannic	609:614	arg1	TA					622:623	TA	622:623	TA	622:623	Tannic acid (TA) was used to construct multiple hydrogen bonds among molecular chains.					
36781283	4	39	theme	Tannic	609:614	arg1	acid					616:619	Tannic acid	609:619	Tannic acid (TA)	609:624	Tannic acid (TA) was used to construct multiple hydrogen bonds among molecular chains.					
36781283	4	40	used	used	630:633	arg2	acid					616:619	Tannic acid	609:619	Tannic acid (TA)	609:624	Tannic acid (TA) was used to construct multiple hydrogen bonds among molecular chains.					
36781283	4	40	used	used	630:633	arg2	TA					622:623	TA	622:623	TA	622:623	Tannic acid (TA) was used to construct multiple hydrogen bonds among molecular chains.					
36781283	4	41	theme	molecular	678:686	arg1	chains					688:693	molecular chains	678:693	molecular chains	678:693	Tannic acid (TA) was used to construct multiple hydrogen bonds among molecular chains.					
36781283	8	42	theme	breathing	1400:1408	arg1	frequency					1410:1418	breathing frequency	1400:1418	breathing frequency	1400:1418	More interestingly, ACP gel can even monitor the cooking process and breathing frequency with fast response, implying applications in food processing, disease diagnosis and medical treatment.					
36781283	3	43	theme	network	491:497	arg1	structure					499:507	a multiple network structure	480:507	a multiple network structure of amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel	480:571	Based on that, a multiple network structure of amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel was prepared by a "cooking" method.					
36781283	7	44	theme	temperature	1188:1198	arg1	changes					1200:1206	temperature changes	1188:1206	temperature changes	1188:1206	Additionally, ACP gel could be served to detect temperature changes with a wide operating range and a high sensitivity of 33 %·°C-1, which is promising to monitor the changes in temperature.					
36781283	6	45	dep	monitor	931:937	arg1	month					1126:1130	a month	1124:1130	a month later	1124:1136	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	6	46	theme	sensor	892:897	arg1	changes					982:988	microscopic expression changes	959:988	microscopic expression changes	959:988	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	6	46	theme	sensor	892:897	arg1	movements					945:953	human movements	939:953	human movements	939:953	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	6	46	theme	sensor	892:897	arg1	device					899:904	a sensor device	890:904	a sensor device	890:904	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	4	47	theme	multiple	648:655	arg1	bonds					666:670	multiple hydrogen bonds	648:670	multiple hydrogen bonds	648:670	Tannic acid (TA) was used to construct multiple hydrogen bonds among molecular chains.					
36781283	5	48	theme	extraordinary	813:825	arg1	-80 °C					855:860	-80 °C	855:860	-80 °C	855:860	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	5	48	theme	extraordinary	813:825	arg1	resistance					843:852	extraordinary low-temperature resistance	813:852	extraordinary low-temperature resistance (-80 °C)	813:861	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	9	49	with	gels	1614:1617	arg1	performance					1633:1643	multiple performance	1624:1643	multiple performance	1624:1643	This study provides new opportunities for the design and fabrication of carbohydrate-based gels with multiple performance and multifunctional electronic devices.					
36781283	9	49	with	gels	1614:1617	arg1	devices					1676:1682	multifunctional electronic devices	1649:1682	multifunctional electronic devices	1649:1682	This study provides new opportunities for the design and fabrication of carbohydrate-based gels with multiple performance and multifunctional electronic devices.					
36781283	8	50	theme	food	1465:1468	arg1	processing					1470:1479	food processing	1465:1479	food processing	1465:1479	More interestingly, ACP gel can even monitor the cooking process and breathing frequency with fast response, implying applications in food processing, disease diagnosis and medical treatment.					
36781283	8	51	theme	medical	1504:1510	arg1	treatment					1512:1520	medical treatment	1504:1520	medical treatment	1504:1520	More interestingly, ACP gel can even monitor the cooking process and breathing frequency with fast response, implying applications in food processing, disease diagnosis and medical treatment.					
36781283	7	52	from	changes	1307:1313	arg1	temperature					1318:1328	temperature	1318:1328	temperature	1318:1328	Additionally, ACP gel could be served to detect temperature changes with a wide operating range and a high sensitivity of 33 %·°C-1, which is promising to monitor the changes in temperature.					
36781283	3	53	theme	ACP	564:566	arg1	gel					569:571	amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel	512:571	amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel	512:571	Based on that, a multiple network structure of amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel was prepared by a "cooking" method.					
36781283	5	54	theme	low-temperature	827:841	arg1	-80 °C					855:860	-80 °C	855:860	-80 °C	855:860	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	5	54	theme	low-temperature	827:841	arg1	resistance					843:852	extraordinary low-temperature resistance	813:852	extraordinary low-temperature resistance (-80 °C)	813:861	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	6	55	theme	expression	971:980	arg1	changes					982:988	microscopic expression changes	959:988	microscopic expression changes	959:988	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	6	55	theme	expression	971:980	arg1	movements					945:953	human movements	939:953	human movements	939:953	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	6	55	theme	expression	971:980	arg1	device					899:904	a sensor device	890:904	a sensor device	890:904	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	5	56	theme	high	717:720	arg1	elongation					722:731	high elongation	717:731	high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing	717:884	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	6	57	theme	human	939:943	arg1	changes					982:988	microscopic expression changes	959:988	microscopic expression changes	959:988	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	6	57	theme	human	939:943	arg1	device					899:904	a sensor device	890:904	a sensor device	890:904	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	6	57	theme	human	939:943	arg1	movements					945:953	human movements	939:953	human movements	939:953	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	7	58	theme	ACP	1154:1156	arg1	gel					1158:1160	ACP gel	1154:1160	ACP gel	1154:1160	Additionally, ACP gel could be served to detect temperature changes with a wide operating range and a high sensitivity of 33 %·°C-1, which is promising to monitor the changes in temperature.					
36781283	6	59	theme	microscopic	959:969	arg1	changes					982:988	microscopic expression changes	959:988	microscopic expression changes	959:988	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	6	59	theme	microscopic	959:969	arg1	movements					945:953	human movements	939:953	human movements	939:953	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	6	59	theme	microscopic	959:969	arg1	device					899:904	a sensor device	890:904	a sensor device	890:904	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
36781283	1	60	from	preparation	243:253	arg1	years					273:277	recent years	266:277	recent years	266:277	Starch with active hydroxyl groups is one of the most attractive carbohydrates for the preparation of gels in recent years.					
36781283	0	61	from	application	117:127	arg1	stimuli-responsiveness					132:153	stimuli-responsiveness	132:153	stimuli-responsiveness	132:153	High-stretchable, self-healing, self-adhesive, self-extinguishing, low-temperature tolerant starch-based gel and its application in stimuli-responsiveness.					
36781283	9	62	theme	multifunctional	1649:1663	arg1	devices					1676:1682	multifunctional electronic devices	1649:1682	multifunctional electronic devices	1649:1682	This study provides new opportunities for the design and fabrication of carbohydrate-based gels with multiple performance and multifunctional electronic devices.					
36781283	5	63	from	self-extinguishing	867:884	arg1	elongation					722:731	high elongation	717:731	high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing	717:884	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	3	64	theme	cellulose-polyacrylamide	538:561	arg1	gel					569:571	amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel	512:571	amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel	512:571	Based on that, a multiple network structure of amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel was prepared by a "cooking" method.					
36781283	9	65	theme	carbohydrate-based	1595:1612	arg1	gels					1614:1617	carbohydrate-based gels	1595:1617	carbohydrate-based gels with multiple performance and multifunctional electronic devices	1595:1682	This study provides new opportunities for the design and fabrication of carbohydrate-based gels with multiple performance and multifunctional electronic devices.					
36781283	8	66	theme	fast	1425:1428	arg1	response					1430:1437	fast response	1425:1437	fast response	1425:1437	More interestingly, ACP gel can even monitor the cooking process and breathing frequency with fast response, implying applications in food processing, disease diagnosis and medical treatment.					
36781283	2	67	theme	self-adhesion	341:353	arg1	properties					304:313	the mechanical properties	289:313	the mechanical properties	289:313	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	2	67	theme	self-adhesion	341:353	arg1	properties					355:364	self-adhesion properties	341:364	self-adhesion properties	341:364	However, the mechanical properties, self-healing properties, self-adhesion properties, especially low-temperature resistance are generally unsatisfactory for current starch-based gels.					
36781283	8	68	theme	ACP	1351:1353	arg1	gel					1355:1357	ACP gel	1351:1357	ACP gel	1351:1357	More interestingly, ACP gel can even monitor the cooking process and breathing frequency with fast response, implying applications in food processing, disease diagnosis and medical treatment.					
36781283	5	69	theme	self-healing	765:776	arg1	performance					778:788	self-healing performance	765:788	self-healing performance	765:788	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	5	70	from	performance	778:788	arg1	elongation					722:731	high elongation	717:731	high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing	717:884	ACP gel demonstrates high elongation at break (1090 %) and strength, self-healing performance and adhesion behavior, extraordinary low-temperature resistance (-80 °C) and self-extinguishing.					
36781283	1	71	theme	active	168:173	arg1	groups					184:189	active hydroxyl groups	168:189	active hydroxyl groups	168:189	Starch with active hydroxyl groups is one of the most attractive carbohydrates for the preparation of gels in recent years.					
36781283	3	72	theme	multiple	482:489	arg1	structure					499:507	a multiple network structure	480:507	a multiple network structure of amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel	480:571	Based on that, a multiple network structure of amylopectin-carboxymethyl cellulose-polyacrylamide (ACP) gel was prepared by a "cooking" method.					
36781283	1	73	theme	gels	258:261	arg1	preparation					243:253	the preparation	239:253	the preparation of gels in recent years	239:277	Starch with active hydroxyl groups is one of the most attractive carbohydrates for the preparation of gels in recent years.					
36781283	7	74	theme	33 	1262:1264	arg1	%					1265:1265	%	1265:1265	%	1265:1265	Additionally, ACP gel could be served to detect temperature changes with a wide operating range and a high sensitivity of 33 %·°C-1, which is promising to monitor the changes in temperature.					
36781283	0	75	dep	High-stretchable	0:15	arg1	self-healing					18:29	self-healing	18:29	self-healing	18:29	High-stretchable, self-healing, self-adhesive, self-extinguishing, low-temperature tolerant starch-based gel and its application in stimuli-responsiveness.					
36781283	0	75	dep	High-stretchable	0:15	arg1	self-extinguishing					47:64	self-extinguishing	47:64	self-extinguishing	47:64	High-stretchable, self-healing, self-adhesive, self-extinguishing, low-temperature tolerant starch-based gel and its application in stimuli-responsiveness.					
36781283	0	75	dep	High-stretchable	0:15	arg1	tolerant					83:90	tolerant	83:90	tolerant	83:90	High-stretchable, self-healing, self-adhesive, self-extinguishing, low-temperature tolerant starch-based gel and its application in stimuli-responsiveness.					
36781283	0	75	dep	High-stretchable	0:15	arg1	self-adhesive					32:44	self-adhesive	32:44	self-adhesive	32:44	High-stretchable, self-healing, self-adhesive, self-extinguishing, low-temperature tolerant starch-based gel and its application in stimuli-responsiveness.					
36781283	1	76	theme	hydroxyl	175:182	arg1	groups					184:189	active hydroxyl groups	168:189	active hydroxyl groups	168:189	Starch with active hydroxyl groups is one of the most attractive carbohydrates for the preparation of gels in recent years.					
36781283	7	77	theme	%	1265:1265	arg1	·°C-1					1266:1270	33 %·°C-1	1262:1270	33 %·°C-1	1262:1270	Additionally, ACP gel could be served to detect temperature changes with a wide operating range and a high sensitivity of 33 %·°C-1, which is promising to monitor the changes in temperature.					
36781283	6	78	theme	multiple	1053:1060	arg1	steps					1078:1082	multiple cutting-healing steps	1053:1082	multiple cutting-healing steps	1053:1082	As a sensor device, ACP gel can effectively monitor human movements and microscopic expression changes and achieve real-time monitoring under harsh conditions (After multiple cutting-healing steps, under low-temperature conditions, even a month later).					
35618320	10	0	theme	bactericidal	2023:2034	arg1	effect					2036:2041	a concentration-dependent bactericidal effect	1997:2041	a concentration-dependent bactericidal effect	1997:2041	The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects.					
35618320	10	1	theme	killing	1974:1980	arg1	curve					1987:1991	The killing rate curve	1970:1991	The killing rate curve	1970:1991	The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects.					
35618320	1	2	theme	colony	306:311	arg1	SASCVs					323:328	SASCVs	323:328	SASCVs	323:328	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	1	2	theme	colony	306:311	arg1	variants					313:320	small colony variants	300:320	Staphylococcus aureus small colony variants (SASCVs)	278:329	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	4	3	theme	antibacterial	1053:1065	arg1	activity					1067:1074	the antibacterial activity	1049:1074	the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain	1049:1149	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	8	4	theme	adhesive	1749:1756	arg1	studies					1758:1764	adhesive studies	1749:1764	adhesive studies	1749:1764	In addition, the enrofloxacin composite nanogels could enhance the bioadhesion capacity of enrofloxacin for the SASCVs strain by adhesive studies.					
35618320	11	5	theme	other	2283:2287	arg1	infections					2313:2322	other intracellular bacterial infections	2283:2322	other intracellular bacterial infections	2283:2322	CONCLUSIONS This study provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections.					
35618320	11	6	theme	enrofloxacin	2190:2201	arg1	nanogels					2213:2220	enrofloxacin composite nanogels	2190:2220	enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections	2190:2322	CONCLUSIONS This study provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections.					
35618320	3	7	dep	the	730:732	arg1	help					734:737	help	734:737	help	734:737	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	9	8	theme	bactericidal	1813:1824	arg1	concentration					1826:1838	minimum bactericidal concentration	1805:1838	minimum bactericidal concentration	1805:1838	The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively.					
35618320	3	9	theme	positive	666:673	arg1	gelatin					657:663	gelatin	657:663	gelatin (positive charge)	657:681	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	9	theme	positive	666:673	arg1	charge					675:680	positive charge	666:680	positive charge	666:680	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	1	10	theme	treatment	234:242	arg1	failure					244:250	treatment failure	234:250	treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs)	234:329	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	4	11	theme	nanogels	1106:1113	arg1	mechanism					968:976	The formation mechanism	954:976	The formation mechanism	954:976	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	4	11	theme	nanogels	1106:1113	arg1	characteristics					990:1004	structural characteristics	979:1004	structural characteristics	979:1004	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	4	11	theme	nanogels	1106:1113	arg1	ability					1019:1025	bioadhesion ability	1007:1025	bioadhesion ability	1007:1025	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	4	11	theme	nanogels	1106:1113	arg1	uptake					1037:1042	cellular uptake	1028:1042	cellular uptake	1028:1042	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	4	11	theme	nanogels	1106:1113	arg1	activity					1067:1074	the antibacterial activity	1049:1074	the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain	1049:1149	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	3	12	theme	single	788:793	arg1	test					802:805	a single factor test	786:805	a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes	786:951	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	6	13	theme	%	1407:1407	arg1	nm					1385:1386	323.2 ± 4.3 nm	1373:1386	323.2 ± 4.3 nm	1373:1386	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	6	13	theme	%	1407:1407	arg1	%					1414:1414	69.6% ± 1.3%	1403:1414	69.6% ± 1.3%	1403:1414	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	11	14	theme	intracellular	2258:2270	arg1	SASCVs					2272:2277	intracellular SASCVs	2258:2277	intracellular SASCVs	2258:2277	CONCLUSIONS This study provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections.					
35618320	4	15	theme	intracellular	1123:1135	arg1	strain					1144:1149	intracellular SASCVs strain	1123:1149	intracellular SASCVs strain	1123:1149	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	3	16	theme	sodium	687:692	arg1	SA					704:705	SA	704:705	SA	704:705	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	16	theme	sodium	687:692	arg1	alginate					694:701	sodium alginate	687:701	sodium alginate (SA; negative charge)	687:723	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	1	17	theme	mastitis	259:266	arg1	failure					244:250	treatment failure	234:250	treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs)	234:329	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	7	18	theme	electron	1477:1484	arg1	microscopy					1486:1495	Transmission electron microscopy	1464:1495	Transmission electron microscopy	1464:1495	Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions.					
35618320	11	19	theme	composite	2203:2211	arg1	nanogels					2213:2220	enrofloxacin composite nanogels	2190:2220	enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections	2190:2322	CONCLUSIONS This study provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections.					
35618320	8	20	theme	enrofloxacin	1637:1648	arg1	nanogels					1660:1667	the enrofloxacin composite nanogels	1633:1667	the enrofloxacin composite nanogels	1633:1667	In addition, the enrofloxacin composite nanogels could enhance the bioadhesion capacity of enrofloxacin for the SASCVs strain by adhesive studies.					
35618320	3	21	with	interaction	637:647	arg1	the					730:732	the	730:732	the	730:732	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	10	22	theme	drug	2057:2060	arg1	concentrations					2062:2075	increasing drug concentrations	2046:2075	increasing drug concentrations	2046:2075	The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects.					
35618320	1	23	theme	poor	171:174	arg1	concentration					190:202	The poor intracellular concentration	167:202	The poor intracellular concentration of enrofloxacin	167:218	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	9	24	theme	inhibitory	1779:1788	arg1	concentration					1790:1802	The minimum inhibitory concentration	1767:1802	The minimum inhibitory concentration	1767:1802	The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively.					
35618320	9	24	theme	inhibitory	1779:1788	arg1	μg/mL					1949:1953	2, 4, 4, and 8 μg/mL	1934:1953	2, 4, 4, and 8 μg/mL	1934:1953	The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively.					
35618320	9	25	theme	biofilm	1895:1901	arg1	concentration					1915:1927	minimum biofilm eradication concentration	1887:1927	minimum biofilm eradication concentration	1887:1927	The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively.					
35618320	3	26	theme	zeta	836:839	arg1	diameter					826:833	the particle diameter	813:833	the particle diameter	813:833	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	26	theme	zeta	836:839	arg1	ZP					852:853	ZP	852:853	ZP	852:853	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	26	theme	zeta	836:839	arg1	EE					938:939	EE	938:939	EE	938:939	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	26	theme	zeta	836:839	arg1	potential					841:849	zeta potential	836:849	zeta potential (ZP)	836:854	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	7	27	theme	good	1586:1589	arg1	size					1600:1603	good particle size	1586:1603	good particle size	1586:1603	Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions.					
35618320	4	28	theme	structural	979:988	arg1	characteristics					990:1004	structural characteristics	979:1004	structural characteristics	979:1004	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	3	29	theme	polydispersity	857:870	arg1	diameter					826:833	the particle diameter	813:833	the particle diameter	813:833	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	29	theme	polydispersity	857:870	arg1	PDI					879:881	PDI	879:881	PDI	879:881	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	29	theme	polydispersity	857:870	arg1	index					872:876	polydispersity index	857:876	polydispersity index (PDI)	857:882	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	0	30	theme	intracellular	97:109	arg1	variants					146:153	intracellular Staphylococcus aureus small colony variants	97:153	intracellular Staphylococcus aureus small colony variants	97:153	Antibacterial activity of enrofloxacin loaded gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants.					
35618320	8	31	theme	bioadhesion	1687:1697	arg1	capacity					1699:1706	the bioadhesion capacity	1683:1706	the bioadhesion capacity of enrofloxacin for the SASCVs strain	1683:1744	In addition, the enrofloxacin composite nanogels could enhance the bioadhesion capacity of enrofloxacin for the SASCVs strain by adhesive studies.					
35618320	1	32	theme	enrofloxacin	207:218	arg1	concentration					190:202	The poor intracellular concentration	167:202	The poor intracellular concentration of enrofloxacin	167:218	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	3	33	theme	loading	885:891	arg1	capacity					893:900	loading capacity	885:900	loading capacity (LC)	885:905	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	33	theme	loading	885:891	arg1	diameter					826:833	the particle diameter	813:833	the particle diameter	813:833	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	33	theme	loading	885:891	arg1	LC					903:904	LC	903:904	LC	903:904	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	0	34	theme	aureus	126:131	arg1	variants					146:153	intracellular Staphylococcus aureus small colony variants	97:153	intracellular Staphylococcus aureus small colony variants	97:153	Antibacterial activity of enrofloxacin loaded gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants.					
35618320	11	35	theme	bacterial	2303:2311	arg1	infections					2313:2322	other intracellular bacterial infections	2283:2322	other intracellular bacterial infections	2283:2322	CONCLUSIONS This study provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections.					
35618320	0	36	theme	colony	139:144	arg1	variants					146:153	intracellular Staphylococcus aureus small colony variants	97:153	intracellular Staphylococcus aureus small colony variants	97:153	Antibacterial activity of enrofloxacin loaded gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants.					
35618320	0	37	theme	gelatin-sodium	46:59	arg1	nanogels					80:87	gelatin-sodium alginate composite nanogels	46:87	gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants	46:153	Antibacterial activity of enrofloxacin loaded gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants.					
35618320	2	38	theme	cow	516:518	arg1	mastitis					520:527	cow mastitis	516:527	cow mastitis caused by intracellular SASCVs	516:558	OBJECTIVES In this study, enrofloxacin composite nanogels were developed to increase the intracellular therapeutic drug concentrations and enhance the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs.					
35618320	5	39	dep	RESULTS	1180:1186	arg1	comprised					1218:1226	comprised	1218:1226	was comprised of 10 mg/mL (gelatin), 5 mg/mL (SA), and 0.25 mg/mL (CaCl2)	1214:1286	RESULTS The optimized formulation was comprised of 10 mg/mL (gelatin), 5 mg/mL (SA), and 0.25 mg/mL (CaCl2).					
35618320	2	40	theme	therapeutic	435:445	arg1	concentrations					452:465	the intracellular therapeutic drug concentrations	417:465	the intracellular therapeutic drug concentrations	417:465	OBJECTIVES In this study, enrofloxacin composite nanogels were developed to increase the intracellular therapeutic drug concentrations and enhance the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs.					
35618320	0	41	theme	Antibacterial	0:12	arg1	activity					14:21	Antibacterial activity	0:21	Antibacterial activity of enrofloxacin	0:37	Antibacterial activity of enrofloxacin loaded gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants.					
35618320	8	42	theme	SASCVs	1732:1737	arg1	strain					1739:1744	the SASCVs strain	1728:1744	the SASCVs strain	1728:1744	In addition, the enrofloxacin composite nanogels could enhance the bioadhesion capacity of enrofloxacin for the SASCVs strain by adhesive studies.					
35618320	2	43	theme	enrofloxacin	495:506	arg1	efficacy					483:490	the efficacy	479:490	the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs	479:558	OBJECTIVES In this study, enrofloxacin composite nanogels were developed to increase the intracellular therapeutic drug concentrations and enhance the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs.					
35618320	10	44	theme	radical	2102:2108	arg1	effects					2118:2124	more radical killing effects	2097:2124	more radical killing effects	2097:2124	The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects.					
35618320	7	45	theme	enrofloxacin	1513:1524	arg1	nanogels					1536:1543	the enrofloxacin composite nanogels	1509:1543	the enrofloxacin composite nanogels	1509:1543	Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions.					
35618320	7	45	theme	enrofloxacin	1513:1524	arg1	spherical					1550:1558	spherical	1550:1558	spherical	1550:1558	Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions.					
35618320	2	46	theme	intracellular	539:551	arg1	SASCVs					553:558	intracellular SASCVs	539:558	intracellular SASCVs	539:558	OBJECTIVES In this study, enrofloxacin composite nanogels were developed to increase the intracellular therapeutic drug concentrations and enhance the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs.					
35618320	11	47	dep	CONCLUSIONS	2127:2137	arg1	provides					2150:2157	provides	2150:2157	provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections	2150:2322	CONCLUSIONS This study provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections.					
35618320	6	48	theme	composite	1349:1357	arg1	nanogels					1359:1366	the optimized enrofloxacin composite nanogels	1322:1366	the optimized enrofloxacin composite nanogels	1322:1366	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	6	49	theme	optimized	1326:1334	arg1	nanogels					1359:1366	the optimized enrofloxacin composite nanogels	1322:1366	the optimized enrofloxacin composite nanogels	1322:1366	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	2	50	theme	composite	371:379	arg1	nanogels					381:388	enrofloxacin composite nanogels	358:388	enrofloxacin composite nanogels	358:388	OBJECTIVES In this study, enrofloxacin composite nanogels were developed to increase the intracellular therapeutic drug concentrations and enhance the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs.					
35618320	4	51	theme	composite	1096:1104	arg1	nanogels					1106:1113	the enrofloxacin composite nanogels	1079:1113	the enrofloxacin composite nanogels	1079:1113	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	6	52	theme	±	1395:1395	arg1	nm					1385:1386	323.2 ± 4.3 nm	1373:1386	323.2 ± 4.3 nm	1373:1386	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	6	52	theme	±	1395:1395	arg1	%					1400:1400	15.4% ± 0.2%	1389:1400	15.4% ± 0.2%	1389:1400	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	1	53	theme	Staphylococcus	278:291	arg1	SASCVs					323:328	SASCVs	323:328	SASCVs	323:328	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	1	53	theme	Staphylococcus	278:291	arg1	variants					313:320	small colony variants	300:320	Staphylococcus aureus small colony variants (SASCVs)	278:329	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	6	54	theme	enrofloxacin	1336:1347	arg1	nanogels					1359:1366	the optimized enrofloxacin composite nanogels	1322:1366	the optimized enrofloxacin composite nanogels	1322:1366	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	10	55	theme	rate	1982:1985	arg1	curve					1987:1991	The killing rate curve	1970:1991	The killing rate curve	1970:1991	The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects.					
35618320	10	56	contain	had	1993:1995	arg1	curve					1987:1991	The killing rate curve	1970:1991	The killing rate curve	1970:1991	The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects.					
35618320	10	56	contain	had	1993:1995	arg2	effect					2036:2041	a concentration-dependent bactericidal effect	1997:2041	a concentration-dependent bactericidal effect	1997:2041	The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects.					
35618320	3	57	theme	composite	582:590	arg1	nanogels					592:599	Enrofloxacin composite nanogels	569:599	METHODS Enrofloxacin composite nanogels	561:599	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	10	58	theme	killing	2110:2116	arg1	effects					2118:2124	more radical killing effects	2097:2124	more radical killing effects	2097:2124	The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects.					
35618320	1	59	theme	small	300:304	arg1	SASCVs					323:328	SASCVs	323:328	SASCVs	323:328	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	1	59	theme	small	300:304	arg1	variants					313:320	small colony variants	300:320	Staphylococcus aureus small colony variants (SASCVs)	278:329	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	9	60	theme	minimum	1771:1777	arg1	concentration					1790:1802	The minimum inhibitory concentration	1767:1802	The minimum inhibitory concentration	1767:1802	The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively.					
35618320	9	60	theme	minimum	1771:1777	arg1	μg/mL					1949:1953	2, 4, 4, and 8 μg/mL	1934:1953	2, 4, 4, and 8 μg/mL	1934:1953	The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively.					
35618320	3	61	theme	ionic	749:753	arg1	crosslinkers					755:766	ionic crosslinkers	749:766	ionic crosslinkers	749:766	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	61	theme	ionic	749:753	arg1	CaCl2					742:746	CaCl2	742:746	CaCl2 (ionic crosslinkers)	742:767	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	62	theme	electrostatic	623:635	arg1	interaction					637:647	an electrostatic interaction	620:647	an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers)	620:767	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	10	63	theme	concentration-dependent	1999:2021	arg1	effect					2036:2041	a concentration-dependent bactericidal effect	1997:2041	a concentration-dependent bactericidal effect	1997:2041	The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects.					
35618320	11	64	theme	cow	2235:2237	arg1	mastitis					2239:2246	cow mastitis	2235:2246	cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections	2235:2322	CONCLUSIONS This study provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections.					
35618320	6	65	theme	0.11	1417:1420	arg1	nm					1385:1386	323.2 ± 4.3 nm	1373:1386	323.2 ± 4.3 nm	1373:1386	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	6	65	theme	0.11	1417:1420	arg1	±					1422:1422	0.11 ± 0.02	1417:1427	0.11 ± 0.02	1417:1427	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	0	66	theme	alginate	61:68	arg1	nanogels					80:87	gelatin-sodium alginate composite nanogels	46:87	gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants	46:153	Antibacterial activity of enrofloxacin loaded gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants.					
35618320	1	67	dep	variants	313:320	arg1	aureus					293:298	Staphylococcus aureus small colony variants (SASCVs)	278:329	Staphylococcus aureus small colony variants (SASCVs)	278:329	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	9	68	theme	minimum	1805:1811	arg1	concentration					1826:1838	minimum bactericidal concentration	1805:1838	minimum bactericidal concentration	1805:1838	The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively.					
35618320	10	69	theme	increasing	2046:2055	arg1	concentrations					2062:2075	increasing drug concentrations	2046:2075	increasing drug concentrations	2046:2075	The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects.					
35618320	6	70	theme	±	1409:1409	arg1	nm					1385:1386	323.2 ± 4.3 nm	1373:1386	323.2 ± 4.3 nm	1373:1386	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	6	70	theme	±	1409:1409	arg1	%					1414:1414	69.6% ± 1.3%	1403:1414	69.6% ± 1.3%	1403:1414	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	4	71	theme	SASCVs	1137:1142	arg1	strain					1144:1149	intracellular SASCVs strain	1123:1149	intracellular SASCVs strain	1123:1149	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	3	72	theme	factor	795:800	arg1	test					802:805	a single factor test	786:805	a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes	786:951	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	73	dep	SA	704:705	arg1	charge					717:722	negative charge	708:722	negative charge	708:722	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	7	74	theme	Transmission	1464:1475	arg1	microscopy					1486:1495	Transmission electron microscopy	1464:1495	Transmission electron microscopy	1464:1495	Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions.					
35618320	9	75	theme	inhibitory	1857:1866	arg1	concentration					1868:1880	minimum biofilm inhibitory concentration	1841:1880	minimum biofilm inhibitory concentration	1841:1880	The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively.					
35618320	7	76	with	spherical	1550:1558	arg1	surface					1574:1580	a smooth surface	1565:1580	a smooth surface	1565:1580	Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions.					
35618320	7	76	with	spherical	1550:1558	arg1	size					1600:1603	good particle size	1586:1603	good particle size	1586:1603	Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions.					
35618320	3	77	theme	particle	817:824	arg1	diameter					826:833	the particle diameter	813:833	the particle diameter	813:833	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	77	theme	particle	817:824	arg1	indexes					945:951	indexes	945:951	indexes	945:951	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	77	theme	particle	817:824	arg1	index					872:876	polydispersity index	857:876	polydispersity index (PDI)	857:882	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	77	theme	particle	817:824	arg1	capacity					893:900	loading capacity	885:900	loading capacity (LC)	885:905	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	77	theme	particle	817:824	arg1	potential					841:849	zeta potential	836:849	zeta potential (ZP)	836:854	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	77	theme	particle	817:824	arg1	efficiency					926:935	encapsulation efficiency	912:935	encapsulation efficiency	912:935	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	6	78	theme	±	1440:1440	arg1	nm					1385:1386	323.2 ± 4.3 nm	1373:1386	323.2 ± 4.3 nm	1373:1386	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	6	78	theme	±	1440:1440	arg1	mV					1446:1447	-34.4 ± 0.8 mV	1434:1447	-34.4 ± 0.8 mV	1434:1447	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	9	79	theme	minimum	1887:1893	arg1	concentration					1915:1927	minimum biofilm eradication concentration	1887:1927	minimum biofilm eradication concentration	1887:1927	The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively.					
35618320	8	80	theme	composite	1650:1658	arg1	nanogels					1660:1667	the enrofloxacin composite nanogels	1633:1667	the enrofloxacin composite nanogels	1633:1667	In addition, the enrofloxacin composite nanogels could enhance the bioadhesion capacity of enrofloxacin for the SASCVs strain by adhesive studies.					
35618320	1	81	theme	intracellular	176:188	arg1	concentration					190:202	The poor intracellular concentration	167:202	The poor intracellular concentration of enrofloxacin	167:218	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	4	82	theme	formation	958:966	arg1	mechanism					968:976	The formation mechanism	954:976	The formation mechanism	954:976	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	9	83	theme	eradication	1903:1913	arg1	concentration					1915:1927	minimum biofilm eradication concentration	1887:1927	minimum biofilm eradication concentration	1887:1927	The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively.					
35618320	7	84	theme	particle	1591:1598	arg1	size					1600:1603	good particle size	1586:1603	good particle size	1586:1603	Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions.					
35618320	0	85	theme	small	133:137	arg1	variants					146:153	intracellular Staphylococcus aureus small colony variants	97:153	intracellular Staphylococcus aureus small colony variants	97:153	Antibacterial activity of enrofloxacin loaded gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants.					
35618320	11	86	theme	good	2161:2164	arg1	tendency					2166:2173	a good tendency	2159:2173	a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections	2159:2322	CONCLUSIONS This study provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections.					
35618320	11	87	theme	intracellular	2289:2301	arg1	infections					2313:2322	other intracellular bacterial infections	2283:2322	other intracellular bacterial infections	2283:2322	CONCLUSIONS This study provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections.					
35618320	9	88	theme	minimum	1841:1847	arg1	concentration					1868:1880	minimum biofilm inhibitory concentration	1841:1880	minimum biofilm inhibitory concentration	1841:1880	The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively.					
35618320	3	89	dep	METHODS	561:567	arg1	nanogels					592:599	Enrofloxacin composite nanogels	569:599	METHODS Enrofloxacin composite nanogels	561:599	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	90	theme	encapsulation	912:924	arg1	diameter					826:833	the particle diameter	813:833	the particle diameter	813:833	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	3	90	theme	encapsulation	912:924	arg1	efficiency					926:935	encapsulation efficiency	912:935	encapsulation efficiency	912:935	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	7	91	dep	surface	1574:1580	arg1	distributions					1605:1617	distributions	1605:1617	distributions	1605:1617	Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions.					
35618320	1	92	dep	BACKGROUND	156:165	arg1	lead					226:229	lead	226:229	might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs)	220:329	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	2	93	theme	drug	447:450	arg1	concentrations					452:465	the intracellular therapeutic drug concentrations	417:465	the intracellular therapeutic drug concentrations	417:465	OBJECTIVES In this study, enrofloxacin composite nanogels were developed to increase the intracellular therapeutic drug concentrations and enhance the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs.					
35618320	8	94	theme	enrofloxacin	1711:1722	arg1	capacity					1699:1706	the bioadhesion capacity	1683:1706	the bioadhesion capacity of enrofloxacin for the SASCVs strain	1683:1744	In addition, the enrofloxacin composite nanogels could enhance the bioadhesion capacity of enrofloxacin for the SASCVs strain by adhesive studies.					
35618320	2	95	theme	intracellular	421:433	arg1	concentrations					452:465	the intracellular therapeutic drug concentrations	417:465	the intracellular therapeutic drug concentrations	417:465	OBJECTIVES In this study, enrofloxacin composite nanogels were developed to increase the intracellular therapeutic drug concentrations and enhance the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs.					
35618320	0	96	theme	enrofloxacin	26:37	arg1	activity					14:21	Antibacterial activity	0:21	Antibacterial activity of enrofloxacin	0:37	Antibacterial activity of enrofloxacin loaded gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants.					
35618320	9	97	theme	biofilm	1849:1855	arg1	concentration					1868:1880	minimum biofilm inhibitory concentration	1841:1880	minimum biofilm inhibitory concentration	1841:1880	The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively.					
35618320	6	98	theme	nanogels	1359:1366	arg1	nm					1385:1386	323.2 ± 4.3 nm	1373:1386	323.2 ± 4.3 nm	1373:1386	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	6	98	theme	nanogels	1359:1366	arg1	LC					1299:1300	LC	1299:1300	LC	1299:1300	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	6	98	theme	nanogels	1359:1366	arg1	ZP					1316:1317	ZP	1316:1317	ZP	1316:1317	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	6	98	theme	nanogels	1359:1366	arg1	EE					1303:1304	EE	1303:1304	EE	1303:1304	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	6	98	theme	nanogels	1359:1366	arg1	PDI					1307:1309	PDI	1307:1309	PDI	1307:1309	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	6	98	theme	nanogels	1359:1366	arg1	size					1293:1296	The size	1289:1296	The size	1289:1296	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	7	99	theme	smooth	1567:1572	arg1	surface					1574:1580	a smooth surface	1565:1580	a smooth surface	1565:1580	Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions.					
35618320	1	100	theme	cow	255:257	arg1	mastitis					259:266	cow mastitis	255:266	cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs)	255:329	BACKGROUND The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs).					
35618320	7	101	theme	composite	1526:1534	arg1	nanogels					1536:1543	the enrofloxacin composite nanogels	1509:1543	the enrofloxacin composite nanogels	1509:1543	Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions.					
35618320	7	101	theme	composite	1526:1534	arg1	spherical					1550:1558	spherical	1550:1558	spherical	1550:1558	Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions.					
35618320	3	102	theme	negative	708:715	arg1	charge					717:722	negative charge	708:722	negative charge	708:722	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	0	103	theme	composite	70:78	arg1	nanogels					80:87	gelatin-sodium alginate composite nanogels	46:87	gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants	46:153	Antibacterial activity of enrofloxacin loaded gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants.					
35618320	4	104	theme	bioadhesion	1007:1017	arg1	ability					1019:1025	bioadhesion ability	1007:1025	bioadhesion ability	1007:1025	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	3	105	theme	Enrofloxacin	569:580	arg1	nanogels					592:599	Enrofloxacin composite nanogels	569:599	METHODS Enrofloxacin composite nanogels	561:599	METHODS Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes.					
35618320	5	106	theme	optimized	1192:1200	arg1	formulation					1202:1212	The optimized formulation	1188:1212	The optimized formulation	1188:1212	RESULTS The optimized formulation was comprised of 10 mg/mL (gelatin), 5 mg/mL (SA), and 0.25 mg/mL (CaCl2).					
35618320	2	107	theme	enrofloxacin	358:369	arg1	nanogels					381:388	enrofloxacin composite nanogels	358:388	enrofloxacin composite nanogels	358:388	OBJECTIVES In this study, enrofloxacin composite nanogels were developed to increase the intracellular therapeutic drug concentrations and enhance the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs.					
35618320	4	108	theme	enrofloxacin	1083:1094	arg1	nanogels					1106:1113	the enrofloxacin composite nanogels	1079:1113	the enrofloxacin composite nanogels	1079:1113	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	4	109	theme	cellular	1028:1035	arg1	uptake					1037:1042	cellular uptake	1028:1042	cellular uptake	1028:1042	The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically.					
35618320	6	110	theme	%	1393:1393	arg1	nm					1385:1386	323.2 ± 4.3 nm	1373:1386	323.2 ± 4.3 nm	1373:1386	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35618320	6	110	theme	%	1393:1393	arg1	%					1400:1400	15.4% ± 0.2%	1389:1400	15.4% ± 0.2%	1389:1400	The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively.					
35881786	9	0	theme	HPSE-driven	1240:1250	arg1	malignancies					1252:1263	pathological HPSE-driven malignancies	1227:1263	pathological HPSE-driven malignancies	1227:1263	We demonstrate the feasibility of such compounds to control pathological HPSE-driven malignancies.					
35881786	3	1	theme	extracellular	387:399	arg1	sulfate					409:415	extracellular heparan sulfate	387:415	extracellular heparan sulfate catabolism	387:426	Heparanase (HPSE) is the principal enzyme responsible for extracellular heparan sulfate catabolism and is markedly up-regulated in aggressive cancers.					
35881786	7	2	link	cyclophellitol-derived	959:980	arg1	inhibitors					987:996	cyclophellitol-derived HPSE inhibitors	959:996	cyclophellitol-derived HPSE inhibitors	959:996	Application of cyclophellitol-derived HPSE inhibitors reduces cancer aggression in cellulo and significantly ameliorates murine metastasis.					
35881786	6	3	theme	physiological	916:928	arg1	environments					930:941	physiological environments	916:941	physiological environments	916:941	Inspired by the natural glycosidase inhibitor cyclophellitol, we developed nanomolar mechanism-based, irreversible HPSE inhibitors that are effective within physiological environments.					
35881786	1	4	theme	Heparan	72:78	arg1	proteoglycans					88:100	Heparan sulfate proteoglycans	72:100	Heparan sulfate proteoglycans (HSPGs)	72:108	Heparan sulfate proteoglycans (HSPGs) mediate essential interactions throughout the extracellular matrix (ECM), providing signals that regulate cellular growth and development.					
35881786	1	4	theme	Heparan	72:78	arg1	HSPGs					103:107	HSPGs	103:107	HSPGs	103:107	Heparan sulfate proteoglycans (HSPGs) mediate essential interactions throughout the extracellular matrix (ECM), providing signals that regulate cellular growth and development.					
35881786	3	5	theme	heparan	401:407	arg1	sulfate					409:415	extracellular heparan sulfate	387:415	extracellular heparan sulfate catabolism	387:426	Heparanase (HPSE) is the principal enzyme responsible for extracellular heparan sulfate catabolism and is markedly up-regulated in aggressive cancers.					
35881786	8	6	theme	irreversible	1100:1111	arg1	strategy					1157:1164	an unexplored anticancer strategy	1132:1164	an unexplored anticancer strategy	1132:1164	Mechanism-based irreversible HPSE inhibition is an unexplored anticancer strategy.					
35881786	8	6	theme	irreversible	1100:1111	arg1	inhibition					1118:1127	Mechanism-based irreversible HPSE inhibition	1084:1127	Mechanism-based irreversible HPSE inhibition	1084:1127	Mechanism-based irreversible HPSE inhibition is an unexplored anticancer strategy.					
35881786	5	7	theme	few	689:691	arg1	inhibitors					703:712	few effective inhibitors	689:712	few effective inhibitors	689:712	Reducing extracellular HPSE activity reduces cancer growth, but few effective inhibitors are known, and none are clinically approved.					
35881786	1	8	theme	sulfate	80:86	arg1	proteoglycans					88:100	Heparan sulfate proteoglycans	72:100	Heparan sulfate proteoglycans (HSPGs)	72:108	Heparan sulfate proteoglycans (HSPGs) mediate essential interactions throughout the extracellular matrix (ECM), providing signals that regulate cellular growth and development.					
35881786	1	8	theme	sulfate	80:86	arg1	HSPGs					103:107	HSPGs	103:107	HSPGs	103:107	Heparan sulfate proteoglycans (HSPGs) mediate essential interactions throughout the extracellular matrix (ECM), providing signals that regulate cellular growth and development.					
35881786	6	9	theme	inhibitor	795:803	arg1	cyclophellitol					805:818	the natural glycosidase inhibitor cyclophellitol	771:818	the natural glycosidase inhibitor cyclophellitol	771:818	Inspired by the natural glycosidase inhibitor cyclophellitol, we developed nanomolar mechanism-based, irreversible HPSE inhibitors that are effective within physiological environments.					
35881786	4	10	dep	degrades	498:505	arg1	facilitating					529:540	facilitating	529:540	facilitating metastatic dissemination	529:565	HPSE overactivity degrades HSPGs within the ECM, facilitating metastatic dissemination and releasing mitogens that drive cellular proliferation.					
35881786	4	10	dep	degrades	498:505	arg1	releasing					571:579	releasing	571:579	releasing mitogens that drive cellular proliferation	571:622	HPSE overactivity degrades HSPGs within the ECM, facilitating metastatic dissemination and releasing mitogens that drive cellular proliferation.					
35881786	3	11	theme	responsible	371:381	arg1	enzyme					364:369	the principal enzyme	350:369	the principal enzyme responsible for extracellular heparan sulfate catabolism	350:426	Heparanase (HPSE) is the principal enzyme responsible for extracellular heparan sulfate catabolism and is markedly up-regulated in aggressive cancers.					
35881786	3	11	theme	responsible	371:381	arg1	Heparanase					329:338	Heparanase	329:338	Heparanase (HPSE)	329:345	Heparanase (HPSE) is the principal enzyme responsible for extracellular heparan sulfate catabolism and is markedly up-regulated in aggressive cancers.					
35881786	5	12	theme	effective	693:701	arg1	inhibitors					703:712	few effective inhibitors	689:712	few effective inhibitors	689:712	Reducing extracellular HPSE activity reduces cancer growth, but few effective inhibitors are known, and none are clinically approved.					
35881786	2	13	theme	cancer	309:314	arg1	progression					316:326	aids cancer progression	304:326	aids cancer progression	304:326	Altered HSPG composition during tumorigenesis strongly aids cancer progression.					
35881786	6	14	theme	glycosidase	783:793	arg1	cyclophellitol					805:818	the natural glycosidase inhibitor cyclophellitol	771:818	the natural glycosidase inhibitor cyclophellitol	771:818	Inspired by the natural glycosidase inhibitor cyclophellitol, we developed nanomolar mechanism-based, irreversible HPSE inhibitors that are effective within physiological environments.					
35881786	4	15	theme	metastatic	542:551	arg1	dissemination					553:565	metastatic dissemination	542:565	metastatic dissemination	542:565	HPSE overactivity degrades HSPGs within the ECM, facilitating metastatic dissemination and releasing mitogens that drive cellular proliferation.					
35881786	2	16	theme	aids	304:307	arg1	progression					316:326	aids cancer progression	304:326	aids cancer progression	304:326	Altered HSPG composition during tumorigenesis strongly aids cancer progression.					
35881786	6	17	theme	natural	775:781	arg1	cyclophellitol					805:818	the natural glycosidase inhibitor cyclophellitol	771:818	the natural glycosidase inhibitor cyclophellitol	771:818	Inspired by the natural glycosidase inhibitor cyclophellitol, we developed nanomolar mechanism-based, irreversible HPSE inhibitors that are effective within physiological environments.					
35881786	0	18	theme	heparanase	16:25	arg1	inhibitors					27:36	Mechanism-based heparanase inhibitors	0:36	Mechanism-based heparanase inhibitors	0:36	Mechanism-based heparanase inhibitors reduce cancer metastasis in vivo.					
35881786	9	19	theme	such	1201:1204	arg1	compounds					1206:1214	such compounds	1201:1214	such compounds	1201:1214	We demonstrate the feasibility of such compounds to control pathological HPSE-driven malignancies.					
35881786	7	20	theme	inhibitors	987:996	arg1	Application					944:954	Application	944:954	Application of cyclophellitol-derived HPSE inhibitors	944:996	Application of cyclophellitol-derived HPSE inhibitors reduces cancer aggression in cellulo and significantly ameliorates murine metastasis.					
35881786	0	21	theme	Mechanism-based	0:14	arg1	inhibitors					27:36	Mechanism-based heparanase inhibitors	0:36	Mechanism-based heparanase inhibitors	0:36	Mechanism-based heparanase inhibitors reduce cancer metastasis in vivo.					
35881786	5	22	theme	cancer	670:675	arg1	growth					677:682	cancer growth	670:682	cancer growth	670:682	Reducing extracellular HPSE activity reduces cancer growth, but few effective inhibitors are known, and none are clinically approved.					
35881786	3	23	theme	sulfate	409:415	arg1	catabolism					417:426	extracellular heparan sulfate catabolism	387:426	extracellular heparan sulfate catabolism	387:426	Heparanase (HPSE) is the principal enzyme responsible for extracellular heparan sulfate catabolism and is markedly up-regulated in aggressive cancers.					
35881786	6	24	theme	HPSE	874:877	arg1	inhibitors					879:888	nanomolar mechanism-based, irreversible HPSE inhibitors	834:888	inhibitors	879:888	Inspired by the natural glycosidase inhibitor cyclophellitol, we developed nanomolar mechanism-based, irreversible HPSE inhibitors that are effective within physiological environments.					
35881786	7	25	theme	cyclophellitol-derived	959:980	arg1	inhibitors					987:996	cyclophellitol-derived HPSE inhibitors	959:996	cyclophellitol-derived HPSE inhibitors	959:996	Application of cyclophellitol-derived HPSE inhibitors reduces cancer aggression in cellulo and significantly ameliorates murine metastasis.					
35881786	6	26	theme	mechanism-based	844:858	arg1	inhibitors					879:888	nanomolar mechanism-based, irreversible HPSE inhibitors	834:888	inhibitors	879:888	Inspired by the natural glycosidase inhibitor cyclophellitol, we developed nanomolar mechanism-based, irreversible HPSE inhibitors that are effective within physiological environments.					
35881786	1	27	theme	essential	118:126	arg1	interactions					128:139	essential interactions	118:139	essential interactions	118:139	Heparan sulfate proteoglycans (HSPGs) mediate essential interactions throughout the extracellular matrix (ECM), providing signals that regulate cellular growth and development.					
35881786	1	28	theme	cellular	216:223	arg1	growth					225:230	growth	225:230	growth	225:230	Heparan sulfate proteoglycans (HSPGs) mediate essential interactions throughout the extracellular matrix (ECM), providing signals that regulate cellular growth and development.					
35881786	7	29	theme	cancer	1006:1011	arg1	aggression					1013:1022	cancer aggression	1006:1022	cancer aggression in cellulo	1006:1033	Application of cyclophellitol-derived HPSE inhibitors reduces cancer aggression in cellulo and significantly ameliorates murine metastasis.					
35881786	8	30	theme	Mechanism-based	1084:1098	arg1	strategy					1157:1164	an unexplored anticancer strategy	1132:1164	an unexplored anticancer strategy	1132:1164	Mechanism-based irreversible HPSE inhibition is an unexplored anticancer strategy.					
35881786	8	30	theme	Mechanism-based	1084:1098	arg1	inhibition					1118:1127	Mechanism-based irreversible HPSE inhibition	1084:1127	Mechanism-based irreversible HPSE inhibition	1084:1127	Mechanism-based irreversible HPSE inhibition is an unexplored anticancer strategy.					
35881786	2	31	theme	HSPG	257:260	arg1	composition					262:272	Altered HSPG composition	249:272	Altered HSPG composition during tumorigenesis	249:293	Altered HSPG composition during tumorigenesis strongly aids cancer progression.					
35881786	0	32	theme	cancer	45:50	arg1	metastasis					52:61	cancer metastasis in vivo	45:69	cancer metastasis in vivo	45:69	Mechanism-based heparanase inhibitors reduce cancer metastasis in vivo.					
35881786	3	33	theme	aggressive	460:469	arg1	cancers					471:477	aggressive cancers	460:477	aggressive cancers	460:477	Heparanase (HPSE) is the principal enzyme responsible for extracellular heparan sulfate catabolism and is markedly up-regulated in aggressive cancers.					
35881786	2	34	theme	Altered	249:255	arg1	composition					262:272	Altered HSPG composition	249:272	Altered HSPG composition during tumorigenesis	249:293	Altered HSPG composition during tumorigenesis strongly aids cancer progression.					
35881786	6	35	theme	irreversible	861:872	arg1	inhibitors					879:888	nanomolar mechanism-based, irreversible HPSE inhibitors	834:888	inhibitors	879:888	Inspired by the natural glycosidase inhibitor cyclophellitol, we developed nanomolar mechanism-based, irreversible HPSE inhibitors that are effective within physiological environments.					
35881786	8	36	theme	anticancer	1146:1155	arg1	strategy					1157:1164	an unexplored anticancer strategy	1132:1164	an unexplored anticancer strategy	1132:1164	Mechanism-based irreversible HPSE inhibition is an unexplored anticancer strategy.					
35881786	8	36	theme	anticancer	1146:1155	arg1	inhibition					1118:1127	Mechanism-based irreversible HPSE inhibition	1084:1127	Mechanism-based irreversible HPSE inhibition	1084:1127	Mechanism-based irreversible HPSE inhibition is an unexplored anticancer strategy.					
35881786	5	37	theme	extracellular	634:646	arg1	activity					653:660	extracellular HPSE activity	634:660	extracellular HPSE activity	634:660	Reducing extracellular HPSE activity reduces cancer growth, but few effective inhibitors are known, and none are clinically approved.					
35881786	7	38	theme	murine	1065:1070	arg1	metastasis					1072:1081	murine metastasis	1065:1081	murine metastasis	1065:1081	Application of cyclophellitol-derived HPSE inhibitors reduces cancer aggression in cellulo and significantly ameliorates murine metastasis.					
35881786	4	39	theme	cellular	601:608	arg1	proliferation					610:622	cellular proliferation	601:622	cellular proliferation	601:622	HPSE overactivity degrades HSPGs within the ECM, facilitating metastatic dissemination and releasing mitogens that drive cellular proliferation.					
35881786	5	40	theme	HPSE	648:651	arg1	activity					653:660	extracellular HPSE activity	634:660	extracellular HPSE activity	634:660	Reducing extracellular HPSE activity reduces cancer growth, but few effective inhibitors are known, and none are clinically approved.					
35881786	9	41	theme	compounds	1206:1214	arg1	feasibility					1186:1196	the feasibility	1182:1196	the feasibility of such compounds to control pathological HPSE-driven malignancies	1182:1263	We demonstrate the feasibility of such compounds to control pathological HPSE-driven malignancies.					
35881786	6	42	theme	nanomolar	834:842	arg1	inhibitors					879:888	nanomolar mechanism-based, irreversible HPSE inhibitors	834:888	inhibitors	879:888	Inspired by the natural glycosidase inhibitor cyclophellitol, we developed nanomolar mechanism-based, irreversible HPSE inhibitors that are effective within physiological environments.					
35881786	7	43	from	aggression	1013:1022	arg1	cellulo					1027:1033	cellulo	1027:1033	cellulo	1027:1033	Application of cyclophellitol-derived HPSE inhibitors reduces cancer aggression in cellulo and significantly ameliorates murine metastasis.					
35881786	1	44	theme	extracellular	156:168	arg1	ECM					178:180	ECM	178:180	ECM	178:180	Heparan sulfate proteoglycans (HSPGs) mediate essential interactions throughout the extracellular matrix (ECM), providing signals that regulate cellular growth and development.					
35881786	1	44	theme	extracellular	156:168	arg1	matrix					170:175	the extracellular matrix	152:175	the extracellular matrix (ECM)	152:181	Heparan sulfate proteoglycans (HSPGs) mediate essential interactions throughout the extracellular matrix (ECM), providing signals that regulate cellular growth and development.					
35881786	7	45	theme	HPSE	982:985	arg1	inhibitors					987:996	cyclophellitol-derived HPSE inhibitors	959:996	cyclophellitol-derived HPSE inhibitors	959:996	Application of cyclophellitol-derived HPSE inhibitors reduces cancer aggression in cellulo and significantly ameliorates murine metastasis.					
35881786	3	46	theme	principal	354:362	arg1	enzyme					364:369	the principal enzyme	350:369	the principal enzyme responsible for extracellular heparan sulfate catabolism	350:426	Heparanase (HPSE) is the principal enzyme responsible for extracellular heparan sulfate catabolism and is markedly up-regulated in aggressive cancers.					
35881786	3	46	theme	principal	354:362	arg1	Heparanase					329:338	Heparanase	329:338	Heparanase (HPSE)	329:345	Heparanase (HPSE) is the principal enzyme responsible for extracellular heparan sulfate catabolism and is markedly up-regulated in aggressive cancers.					
35881786	4	47	theme	HPSE	480:483	arg1	overactivity					485:496	HPSE overactivity	480:496	HPSE overactivity	480:496	HPSE overactivity degrades HSPGs within the ECM, facilitating metastatic dissemination and releasing mitogens that drive cellular proliferation.					
35881786	8	48	theme	unexplored	1135:1144	arg1	strategy					1157:1164	an unexplored anticancer strategy	1132:1164	an unexplored anticancer strategy	1132:1164	Mechanism-based irreversible HPSE inhibition is an unexplored anticancer strategy.					
35881786	8	48	theme	unexplored	1135:1144	arg1	inhibition					1118:1127	Mechanism-based irreversible HPSE inhibition	1084:1127	Mechanism-based irreversible HPSE inhibition	1084:1127	Mechanism-based irreversible HPSE inhibition is an unexplored anticancer strategy.					
35881786	8	49	theme	HPSE	1113:1116	arg1	strategy					1157:1164	an unexplored anticancer strategy	1132:1164	an unexplored anticancer strategy	1132:1164	Mechanism-based irreversible HPSE inhibition is an unexplored anticancer strategy.					
35881786	8	49	theme	HPSE	1113:1116	arg1	inhibition					1118:1127	Mechanism-based irreversible HPSE inhibition	1084:1127	Mechanism-based irreversible HPSE inhibition	1084:1127	Mechanism-based irreversible HPSE inhibition is an unexplored anticancer strategy.					
35881786	9	50	theme	pathological	1227:1238	arg1	malignancies					1252:1263	pathological HPSE-driven malignancies	1227:1263	pathological HPSE-driven malignancies	1227:1263	We demonstrate the feasibility of such compounds to control pathological HPSE-driven malignancies.					
35698373	1	0	theme	high	181:184	arg1	concentration					186:198	high concentration	181:198	high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids	181:276	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	6	1	from	processing	1112:1121	arg1	mixer					1134:1138	a batch mixer	1126:1138	a batch mixer	1126:1138	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
35698373	1	2	theme	nitric	265:270	arg1	acids					272:276	hydrochloric, phosphoric, and nitric acids	235:276	hydrochloric, phosphoric, and nitric acids	235:276	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	5	3	with	competitive	909:919	arg1	those					926:930	those	926:930	those	926:930	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	3	4	theme	molten	542:547	arg1	acid					556:559	molten oxalic acid dihydrate	542:569	molten oxalic acid dihydrate (OA)	542:574	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	3	4	theme	molten	542:547	arg1	OA					572:573	OA	572:573	OA	572:573	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	5	5	theme	produced	878:885	arg1	CNCs					887:890	the produced CNCs	874:890	the produced CNCs	874:890	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	5	6	theme	CNCs	887:890	arg1	degree					824:829	degree	824:829	degree of crystallinity	824:846	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	5	6	theme	CNCs	887:890	arg1	size					818:821	the particle size	805:821	the particle size	805:821	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	5	6	theme	CNCs	887:890	arg1	stability					861:869	thermal stability	853:869	thermal stability of the produced CNCs	853:890	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	5	6	theme	CNCs	887:890	arg1	competitive					909:919	competitive	909:919	competitive	909:919	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	4	7	theme	1:0.7:0.075	694:704	arg1	time					752:755	a processing time	739:755	a processing time of 2.5 min	739:766	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	4	7	theme	1:0.7:0.075	694:704	arg1	composition					679:689	a mixture composition	669:689	a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w)	669:733	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	3	8	theme	oxalic	549:554	arg1	acid					556:559	molten oxalic acid dihydrate	542:569	molten oxalic acid dihydrate (OA)	542:574	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	3	8	theme	oxalic	549:554	arg1	OA					572:573	OA	572:573	OA	572:573	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	5	9	theme	thermal	853:859	arg1	stability					861:869	thermal stability	853:869	thermal stability of the produced CNCs	853:890	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	4	10	dep	Cellulose	707:715	arg1	BmimCl					717:722	BmimCl	717:722	BmimCl	717:722	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	4	10	dep	Cellulose	707:715	arg1	OA					724:725	OA	724:725	OA	724:725	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	3	11	from	cellulose	597:605	arg1	CNCs					587:590	CNCs	587:590	CNCs from cellulose	587:605	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	3	12	theme	reactive	423:430	arg1	process					432:438	a batch mixer reactive process	409:438	a batch mixer reactive process that entails high shear	409:462	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	4	13	theme	2.5 min	760:766	arg1	time					752:755	a processing time	739:755	a processing time of 2.5 min	739:766	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	4	13	theme	2.5 min	760:766	arg1	composition					679:689	a mixture composition	669:689	a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w)	669:733	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	6	14	theme	acid	1065:1068	arg1	treatment					1070:1078	an efficient ionic liquid and molten organic acid treatment	1020:1078	an efficient ionic liquid and molten organic acid treatment for CNC production	1020:1097	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
35698373	6	15	theme	CNC	1084:1086	arg1	production					1088:1097	CNC production	1084:1097	CNC production	1084:1097	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
35698373	1	16	theme	sulfuric	203:210	arg1	acids					272:276	hydrochloric, phosphoric, and nitric acids	235:276	hydrochloric, phosphoric, and nitric acids	235:276	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	1	16	theme	sulfuric	203:210	arg1	acids					221:225	sulfuric or other acids	203:225	sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids	203:276	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	3	17	dep	chloride	514:521	arg1	media					532:536	media	532:536	media	532:536	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	3	18	theme	batch	411:415	arg1	process					432:438	a batch mixer reactive process	409:438	a batch mixer reactive process that entails high shear	409:462	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	0	19	theme	Thermo-mechano-chemical	0:22	arg1	deconstruction					24:37	Thermo-mechano-chemical deconstruction	0:37	Thermo-mechano-chemical deconstruction of cellulose for cellulose nanocrystal production by reactive processing.	0:111	Thermo-mechano-chemical deconstruction of cellulose for cellulose nanocrystal production by reactive processing.					
35698373	1	20	theme	commercial	117:126	arg1	production					128:137	The commercial production	113:137	The commercial production of cellulose nanocrystals (CNCs)	113:170	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	3	21	theme	mixer	417:421	arg1	process					432:438	a batch mixer reactive process	409:438	a batch mixer reactive process that entails high shear	409:462	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	0	22	theme	cellulose	42:50	arg1	deconstruction					24:37	Thermo-mechano-chemical deconstruction	0:37	Thermo-mechano-chemical deconstruction of cellulose for cellulose nanocrystal production by reactive processing.	0:111	Thermo-mechano-chemical deconstruction of cellulose for cellulose nanocrystal production by reactive processing.					
35698373	6	23	theme	potential	984:992	arg1	application					1005:1015	the potential industrial application	980:1015	the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production	980:1097	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
35698373	2	24	theme	involved	308:315	arg1	corrosive					329:337	corrosive	329:337	corrosive	329:337	However, these acids and the involved process are corrosive, toxic, energy-intensive, and not environmentally safe.					
35698373	2	24	theme	involved	308:315	arg1	process					317:323	the involved process	304:323	the involved process	304:323	However, these acids and the involved process are corrosive, toxic, energy-intensive, and not environmentally safe.					
35698373	1	25	theme	acids	221:225	arg1	concentration					186:198	high concentration	181:198	high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids	181:276	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	6	26	theme	treatment	1070:1078	arg1	application					1005:1015	the potential industrial application	980:1015	the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production	980:1097	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
35698373	6	27	theme	reactive	1103:1110	arg1	processing					1112:1121	reactive processing	1103:1121	reactive processing in a batch mixer	1103:1138	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
35698373	3	28	theme	dihydrate	561:569	arg1	acid					556:559	molten oxalic acid dihydrate	542:569	molten oxalic acid dihydrate (OA)	542:574	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	3	28	theme	dihydrate	561:569	arg1	OA					572:573	OA	572:573	OA	572:573	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	0	29	theme	cellulose	56:64	arg1	production					78:87	cellulose nanocrystal production	56:87	cellulose nanocrystal production by reactive processing	56:110	Thermo-mechano-chemical deconstruction of cellulose for cellulose nanocrystal production by reactive processing.					
35698373	3	30	theme	1-butyl-3-methylimidazolium	486:512	arg1	BmimCl					524:529	BmimCl	524:529	BmimCl	524:529	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	3	30	theme	1-butyl-3-methylimidazolium	486:512	arg1	chloride					514:521	1-butyl-3-methylimidazolium chloride	486:521	1-butyl-3-methylimidazolium chloride (BmimCl)	486:530	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	5	31	theme	Further	769:775	arg1	investigation					777:789	Further investigation	769:789	Further investigation	769:789	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	4	32	theme	processing	741:750	arg1	time					752:755	a processing time	739:755	a processing time of 2.5 min	739:766	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	1	33	theme	other	215:219	arg1	acids					272:276	hydrochloric, phosphoric, and nitric acids	235:276	hydrochloric, phosphoric, and nitric acids	235:276	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	1	33	theme	other	215:219	arg1	acids					221:225	sulfuric or other acids	203:225	sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids	203:276	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	4	34	theme	maximum	624:630	arg1	yield					636:640	a maximum CNC yield	622:640	a maximum CNC yield (59 wt%)	622:649	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	4	34	theme	maximum	624:630	arg1	%					648:648	59 wt%	643:648	59 wt%	643:648	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	6	35	theme	industrial	994:1003	arg1	application					1005:1015	the potential industrial application	980:1015	the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production	980:1097	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
35698373	1	36	theme	hydrochloric	235:246	arg1	acids					272:276	hydrochloric, phosphoric, and nitric acids	235:276	hydrochloric, phosphoric, and nitric acids	235:276	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	5	37	theme	commercial	937:946	arg1	product					952:958	a commercial CNC product	935:958	a commercial CNC product	935:958	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	6	38	theme	liquid	1039:1044	arg1	treatment					1070:1078	an efficient ionic liquid and molten organic acid treatment	1020:1078	an efficient ionic liquid and molten organic acid treatment for CNC production	1020:1097	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
35698373	1	39	theme	cellulose	142:150	arg1	CNCs					166:169	CNCs	166:169	CNCs	166:169	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	1	39	theme	cellulose	142:150	arg1	nanocrystals					152:163	cellulose nanocrystals	142:163	cellulose nanocrystals (CNCs)	142:170	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	0	40	theme	nanocrystal	66:76	arg1	production					78:87	cellulose nanocrystal production	56:87	cellulose nanocrystal production by reactive processing	56:110	Thermo-mechano-chemical deconstruction of cellulose for cellulose nanocrystal production by reactive processing.					
35698373	5	41	theme	CNC	948:950	arg1	product					952:958	a commercial CNC product	935:958	a commercial CNC product	935:958	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	1	42	theme	nanocrystals	152:163	arg1	production					128:137	The commercial production	113:137	The commercial production of cellulose nanocrystals (CNCs)	113:170	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	6	43	theme	batch	1128:1132	arg1	mixer					1134:1138	a batch mixer	1126:1138	a batch mixer	1126:1138	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
35698373	4	44	dep	1:0.7:0.075	694:704	arg1	Cellulose					707:715	Cellulose	707:715	Cellulose	707:715	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	4	44	dep	1:0.7:0.075	694:704	arg1	w/w/w					728:732	w/w/w	728:732	w/w/w	728:732	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	5	45	theme	particle	809:816	arg1	size					818:821	the particle size	805:821	the particle size	805:821	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	5	45	theme	particle	809:816	arg1	competitive					909:919	competitive	909:919	competitive	909:919	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	5	46	theme	crystallinity	834:846	arg1	degree					824:829	degree	824:829	degree of crystallinity	824:846	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	5	46	theme	crystallinity	834:846	arg1	size					818:821	the particle size	805:821	the particle size	805:821	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	5	46	theme	crystallinity	834:846	arg1	stability					861:869	thermal stability	853:869	thermal stability of the produced CNCs	853:890	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	5	46	theme	crystallinity	834:846	arg1	competitive					909:919	competitive	909:919	competitive	909:919	Further investigation revealed that the particle size, degree of crystallinity, and thermal stability of the produced CNCs were found to be competitive with those of a commercial CNC product.					
35698373	2	47	dep	environmentally	373:387	arg1	not					369:371	not	369:371	not	369:371	However, these acids and the involved process are corrosive, toxic, energy-intensive, and not environmentally safe.					
35698373	4	48	theme	mixture	671:677	arg1	composition					679:689	a mixture composition	669:689	a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w)	669:733	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	6	49	theme	molten	1050:1055	arg1	treatment					1070:1078	an efficient ionic liquid and molten organic acid treatment	1020:1078	an efficient ionic liquid and molten organic acid treatment for CNC production	1020:1097	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
35698373	1	50	theme	phosphoric	249:258	arg1	acids					272:276	hydrochloric, phosphoric, and nitric acids	235:276	hydrochloric, phosphoric, and nitric acids	235:276	The commercial production of cellulose nanocrystals (CNCs) requires high concentration of sulfuric or other acids such as hydrochloric, phosphoric, and nitric acids.					
35698373	0	51	theme	reactive	92:99	arg1	processing					101:110	reactive processing	92:110	reactive processing	92:110	Thermo-mechano-chemical deconstruction of cellulose for cellulose nanocrystal production by reactive processing.					
35698373	3	52	theme	high	453:456	arg1	shear					458:462	high shear	453:462	high shear	453:462	In this work, a batch mixer reactive process that entails high shear was implemented using 1-butyl-3-methylimidazolium chloride (BmimCl) media and molten oxalic acid dihydrate (OA) to produce CNCs from cellulose.					
35698373	6	53	theme	efficient	1023:1031	arg1	treatment					1070:1078	an efficient ionic liquid and molten organic acid treatment	1020:1078	an efficient ionic liquid and molten organic acid treatment for CNC production	1020:1097	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
35698373	4	54	theme	CNC	632:634	arg1	yield					636:640	a maximum CNC yield	622:640	a maximum CNC yield (59 wt%)	622:649	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	4	54	theme	CNC	632:634	arg1	%					648:648	59 wt%	643:648	59 wt%	643:648	Through this, a maximum CNC yield (59 wt%) was obtained with a mixture composition of 1:0.7:0.075 (Cellulose:BmimCl:OA, w/w/w) and a processing time of 2.5 min.					
35698373	6	55	theme	organic	1057:1063	arg1	treatment					1070:1078	an efficient ionic liquid and molten organic acid treatment	1020:1078	an efficient ionic liquid and molten organic acid treatment for CNC production	1020:1097	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
35698373	6	56	theme	ionic	1033:1037	arg1	treatment					1070:1078	an efficient ionic liquid and molten organic acid treatment	1020:1078	an efficient ionic liquid and molten organic acid treatment for CNC production	1020:1097	This study asserts the potential industrial application of an efficient ionic liquid and molten organic acid treatment for CNC production via reactive processing in a batch mixer.					
36200072	2	0	theme	several	428:434	arg1	polysaccharides					454:468	several animal-originated polysaccharides	428:468	several animal-originated polysaccharides	428:468	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	7	1	theme	polysaccharides	1205:1219	arg1	Eleven					1189:1194	Eleven	1189:1194	Eleven	1189:1194	Eleven of these polysaccharides showed strong binding to PF4.					
36200072	7	1	theme	polysaccharides	1205:1219	arg1	polysaccharides					1205:1219	these polysaccharides	1199:1219	these polysaccharides	1199:1219	Eleven of these polysaccharides showed strong binding to PF4.					
36200072	6	2	with	polysaccharides	1069:1083	arg1	affinities					1108:1117	strong AT-binding affinities	1090:1117	strong AT-binding affinities	1090:1117	Fourteen polysaccharides with strong AT-binding affinities were selected to further investigate their binding affinity with PF4.					
36200072	9	3	theme	sulfation	1418:1426	arg1	contribution					1494:1505	the primary contribution	1482:1505	the primary contribution to strong AT and PF4 interaction	1482:1538	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	9	3	theme	sulfation	1418:1426	arg1	levels					1428:1433	The sulfation levels	1414:1433	The sulfation levels	1414:1433	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	9	3	theme	sulfation	1418:1426	arg1	patterns					1449:1456	sulfation patterns	1439:1456	sulfation patterns	1439:1456	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	8	4	theme	molecular	1302:1310	arg1	monosaccharides					1285:1299	monosaccharides	1285:1299	monosaccharides	1285:1299	It was observed that the types of monosaccharides, molecular weight and branching are not very essential particularly when these polysaccharides are oversulfated.					
36200072	8	4	theme	molecular	1302:1310	arg1	weight					1312:1317	molecular weight	1302:1317	molecular weight	1302:1317	It was observed that the types of monosaccharides, molecular weight and branching are not very essential particularly when these polysaccharides are oversulfated.					
36200072	9	5	theme	PF4	1524:1526	arg1	interaction					1528:1538	PF4 interaction	1524:1538	PF4 interaction	1524:1538	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	3	6	theme	linear	851:856	arg1	chains					858:863	branching vs. linear chains	837:863	branching vs. linear chains	837:863	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	3	7	theme	multiple	650:657	arg1	characteristics					659:673	multiple characteristics	650:673	multiple characteristics	650:673	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	2	8	theme	molecular	559:567	arg1	interactions					569:580	their specific molecular interactions	544:580	their specific molecular interactions with AT and PF4 using surface plasmon resonance	544:628	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	1	9	theme	certain	215:221	arg1	heparin					269:275	heparin	269:275	heparin	269:275	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	1	9	theme	certain	215:221	arg1	events					234:239	certain biological events	215:239	certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT)	215:306	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	1	9	theme	certain	215:221	arg1	anticoagulation					249:263	anticoagulation	249:263	anticoagulation	249:263	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	3	10	theme	different	701:709	arg1	species					717:723	different algal species	701:723	different algal species	701:723	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	2	11	theme	specific	550:557	arg1	interactions					569:580	their specific molecular interactions	544:580	their specific molecular interactions with AT and PF4 using surface plasmon resonance	544:628	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	2	12	theme	plasmon	612:618	arg1	resonance					620:628	surface plasmon resonance	604:628	surface plasmon resonance	604:628	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	4	13	with	affinity	916:923	arg1	AT					953:954	AT	953:954	AT	953:954	These factors were found to influence the binding affinity of the studied glycans with AT.					
36200072	1	14	theme	biological	223:232	arg1	heparin					269:275	heparin	269:275	heparin	269:275	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	1	14	theme	biological	223:232	arg1	events					234:239	certain biological events	215:239	certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT)	215:306	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	1	14	theme	biological	223:232	arg1	anticoagulation					249:263	anticoagulation	249:263	anticoagulation	249:263	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	4	15	theme	glycans	940:946	arg1	affinity					916:923	the binding affinity	904:923	the binding affinity of the studied glycans with AT	904:954	These factors were found to influence the binding affinity of the studied glycans with AT.					
36200072	3	16	theme	algal	711:715	arg1	species					717:723	different algal species	701:723	different algal species	701:723	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	2	17	theme	surface	604:610	arg1	resonance					620:628	surface plasmon resonance	604:628	surface plasmon resonance	604:628	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	9	18	theme	sulfation	1439:1447	arg1	contribution					1494:1505	the primary contribution	1482:1505	the primary contribution to strong AT and PF4 interaction	1482:1538	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	9	18	theme	sulfation	1439:1447	arg1	levels					1428:1433	The sulfation levels	1414:1433	The sulfation levels	1414:1433	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	9	18	theme	sulfation	1439:1447	arg1	patterns					1449:1456	sulfation patterns	1439:1456	sulfation patterns	1439:1456	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	3	19	theme	sulfate	805:811	arg1	content					813:819	sulfate content	805:819	sulfate content	805:819	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	9	20	from	contribution	1494:1505	arg1	hand					1476:1479	the other hand	1466:1479	the other hand	1466:1479	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	5	21	theme	Many	957:960	arg1	polysaccharides					962:976	Many polysaccharides	957:976	Many polysaccharides	957:976	Many polysaccharides showed stronger binding than the low molecular weight heparin (e.g., enoxaparin).					
36200072	1	22	with	interactions	95:106	arg1	AT					165:166	AT	165:166	AT	165:166	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	1	22	with	interactions	95:106	arg1	antithrombin					151:162	antithrombin	151:162	antithrombin (AT)	151:167	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	1	22	with	interactions	95:106	arg1	factor					182:187	platelet factor 4	173:189	platelet factor 4 (PF4)	173:195	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	1	22	with	interactions	95:106	arg1	PF4					192:194	PF4	192:194	PF4	192:194	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	6	23	with	affinity	1170:1177	arg1	PF4					1184:1186	PF4	1184:1186	PF4	1184:1186	Fourteen polysaccharides with strong AT-binding affinities were selected to further investigate their binding affinity with PF4.					
36200072	2	24	with	interactions	569:580	arg1	PF4					594:596	PF4	594:596	PF4	594:596	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	2	24	with	interactions	569:580	arg1	AT					587:588	AT	587:588	AT	587:588	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	3	25	theme	different	726:734	arg1	methods					736:742	different methods	726:742	different methods of extraction	726:756	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	1	26	theme	molecular	85:93	arg1	interactions					95:106	The molecular interactions	81:106	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4)	81:195	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	1	26	theme	molecular	85:93	arg1	essential					201:209	essential	201:209	essential	201:209	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	0	27	theme	sulfated	23:30	arg1	glycans					32:38	marine sulfated glycans	16:38	marine sulfated glycans	16:38	Interactions of marine sulfated glycans with antithrombin and platelet factor 4.					
36200072	8	28	theme	monosaccharides	1285:1299	arg1	types					1276:1280	the types	1272:1280	the types of monosaccharides, molecular weight and branching	1272:1331	It was observed that the types of monosaccharides, molecular weight and branching are not very essential particularly when these polysaccharides are oversulfated.					
36200072	8	28	theme	monosaccharides	1285:1299	arg1	essential					1346:1354	essential	1346:1354	essential	1346:1354	It was observed that the types of monosaccharides, molecular weight and branching are not very essential particularly when these polysaccharides are oversulfated.					
36200072	5	29	theme	weight	1025:1030	arg1	heparin					1032:1038	the low molecular weight heparin	1007:1038	the low molecular weight heparin (e.g., enoxaparin)	1007:1057	Many polysaccharides showed stronger binding than the low molecular weight heparin (e.g., enoxaparin).					
36200072	9	30	theme	other	1470:1474	arg1	hand					1476:1479	the other hand	1466:1479	the other hand	1466:1479	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	0	31	theme	marine	16:21	arg1	glycans					32:38	marine sulfated glycans	16:38	marine sulfated glycans	16:38	Interactions of marine sulfated glycans with antithrombin and platelet factor 4.					
36200072	1	32	theme	platelet	173:180	arg1	factor					182:187	platelet factor 4	173:189	platelet factor 4 (PF4)	173:195	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	1	32	theme	platelet	173:180	arg1	PF4					192:194	PF4	192:194	PF4	192:194	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	9	33	theme	primary	1486:1492	arg1	contribution					1494:1505	the primary contribution	1482:1505	the primary contribution to strong AT and PF4 interaction	1482:1538	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	9	33	theme	primary	1486:1492	arg1	levels					1428:1433	The sulfation levels	1414:1433	The sulfation levels	1414:1433	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	9	33	theme	primary	1486:1492	arg1	patterns					1449:1456	sulfation patterns	1439:1456	sulfation patterns	1439:1456	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	2	34	theme	relationship	509:520	arg1	study					528:532	a structure-activity relationship (SAR) study	488:532	a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance	488:628	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	2	35	dep	glycans	356:362	arg1	oligomers					378:386	oligomers	378:386	oligomers	378:386	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	2	35	dep	glycans	356:362	arg1	polymers					365:372	polymers	365:372	polymers	365:372	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	0	36	theme	glycans	32:38	arg1	Interactions					0:11	Interactions	0:11	Interactions of marine sulfated glycans with antithrombin and platelet factor 4	0:78	Interactions of marine sulfated glycans with antithrombin and platelet factor 4.					
36200072	2	37	theme	structure-activity	490:507	arg1	SAR					523:525	SAR	523:525	SAR	523:525	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	2	37	theme	structure-activity	490:507	arg1	relationship					509:520	a structure-activity relationship	488:520	a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance	488:628	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	2	38	theme	sulfated	347:354	arg1	glycans					356:362	84 sulfated glycans	344:362	84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides	344:468	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	2	39	theme	marine	404:409	arg1	algae					411:415	marine algae	404:415	marine algae	404:415	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	3	40	theme	molecular	759:767	arg1	weight					769:774	molecular weight	759:774	molecular weight	759:774	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	2	41	theme	animal-originated	436:452	arg1	polysaccharides					454:468	several animal-originated polysaccharides	428:468	several animal-originated polysaccharides	428:468	In this study, a library including 84 sulfated glycans (polymers and oligomers) extracted from marine algae along with several animal-originated polysaccharides were subjected to a structure-activity relationship (SAR) study regarding their specific molecular interactions with AT and PF4 using surface plasmon resonance.					
36200072	5	42	theme	low	1011:1013	arg1	heparin					1032:1038	the low molecular weight heparin	1007:1038	the low molecular weight heparin (e.g., enoxaparin)	1007:1057	Many polysaccharides showed stronger binding than the low molecular weight heparin (e.g., enoxaparin).					
36200072	1	43	theme	sulfated	111:118	arg1	heparin					137:143	heparin	137:143	heparin	137:143	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	1	43	theme	sulfated	111:118	arg1	glycans					120:126	sulfated glycans	111:126	sulfated glycans	111:126	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	4	44	theme	binding	908:914	arg1	affinity					916:923	the binding affinity	904:923	the binding affinity of the studied glycans with AT	904:954	These factors were found to influence the binding affinity of the studied glycans with AT.					
36200072	3	45	theme	SAR	639:641	arg1	study					643:647	this SAR study	634:647	this SAR study	634:647	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	5	46	theme	molecular	1015:1023	arg1	heparin					1032:1038	the low molecular weight heparin	1007:1038	the low molecular weight heparin (e.g., enoxaparin)	1007:1057	Many polysaccharides showed stronger binding than the low molecular weight heparin (e.g., enoxaparin).					
36200072	1	47	theme	glycans	120:126	arg1	interactions					95:106	The molecular interactions	81:106	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4)	81:195	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	1	47	theme	glycans	120:126	arg1	essential					201:209	essential	201:209	essential	201:209	The molecular interactions of sulfated glycans, such as heparin, with antithrombin (AT) and platelet factor 4 (PF4) are essential for certain biological events such as anticoagulation and heparin induced thrombocytopenia (HIT).					
36200072	3	48	theme	branching	837:845	arg1	chains					858:863	branching vs. linear chains	837:863	branching vs. linear chains	837:863	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	3	49	theme	extraction	747:756	arg1	pattern					825:831	pattern	825:831	pattern	825:831	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	3	49	theme	extraction	747:756	arg1	composition					792:802	monosaccharide composition	777:802	monosaccharide composition	777:802	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	3	49	theme	extraction	747:756	arg1	species					717:723	different algal species	701:723	different algal species	701:723	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	3	49	theme	extraction	747:756	arg1	content					813:819	sulfate content	805:819	sulfate content	805:819	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	3	49	theme	extraction	747:756	arg1	chains					858:863	branching vs. linear chains	837:863	branching vs. linear chains	837:863	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	3	49	theme	extraction	747:756	arg1	weight					769:774	molecular weight	759:774	molecular weight	759:774	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	3	49	theme	extraction	747:756	arg1	methods					736:742	different methods	726:742	different methods of extraction	726:756	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	0	50	with	Interactions	0:11	arg1	antithrombin					45:56	antithrombin	45:56	antithrombin	45:56	Interactions of marine sulfated glycans with antithrombin and platelet factor 4.					
36200072	0	50	with	Interactions	0:11	arg1	factor					71:76	platelet factor 4	62:78	platelet factor 4	62:78	Interactions of marine sulfated glycans with antithrombin and platelet factor 4.					
36200072	3	51	theme	monosaccharide	777:790	arg1	composition					792:802	monosaccharide composition	777:802	monosaccharide composition	777:802	In this SAR study, multiple characteristics were considered including different algal species, different methods of extraction, molecular weight, monosaccharide composition, sulfate content and pattern and branching vs. linear chains.					
36200072	6	52	theme	binding	1162:1168	arg1	affinity					1170:1177	their binding affinity	1156:1177	their binding affinity with PF4	1156:1186	Fourteen polysaccharides with strong AT-binding affinities were selected to further investigate their binding affinity with PF4.					
36200072	6	53	theme	AT-binding	1097:1106	arg1	affinities					1108:1117	strong AT-binding affinities	1090:1117	strong AT-binding affinities	1090:1117	Fourteen polysaccharides with strong AT-binding affinities were selected to further investigate their binding affinity with PF4.					
36200072	7	54	theme	strong	1228:1233	arg1	binding					1235:1241	strong binding	1228:1241	strong binding to PF4	1228:1248	Eleven of these polysaccharides showed strong binding to PF4.					
36200072	5	55	theme	stronger	985:992	arg1	binding					994:1000	stronger binding	985:1000	stronger binding	985:1000	Many polysaccharides showed stronger binding than the low molecular weight heparin (e.g., enoxaparin).					
36200072	9	56	theme	strong	1510:1515	arg1	AT					1517:1518	strong AT	1510:1518	strong AT	1510:1518	The sulfation levels and sulfation patterns are, on the other hand, the primary contribution to strong AT and PF4 interaction.					
36200072	6	57	theme	strong	1090:1095	arg1	affinities					1108:1117	strong AT-binding affinities	1090:1117	strong AT-binding affinities	1090:1117	Fourteen polysaccharides with strong AT-binding affinities were selected to further investigate their binding affinity with PF4.					
36200072	0	58	theme	platelet	62:69	arg1	factor					71:76	platelet factor 4	62:78	platelet factor 4	62:78	Interactions of marine sulfated glycans with antithrombin and platelet factor 4.					
36200072	4	59	theme	studied	932:938	arg1	glycans					940:946	the studied glycans	928:946	the studied glycans	928:946	These factors were found to influence the binding affinity of the studied glycans with AT.					
36200072	5	60	dep	enoxaparin	1047:1056	arg1	e.g.					1041:1044	e.g.	1041:1044	e.g.	1041:1044	Many polysaccharides showed stronger binding than the low molecular weight heparin (e.g., enoxaparin).					
36799350	0	0	theme	pea	93:95	arg1	beads					105:109	pea protein beads	93:109	pea protein beads	93:109	Production and characterization of Ziziphus jujuba extract-loaded composite whey protein and pea protein beads based on Na-alginate/IFPG (insoluble fraction of Persian gum).					
36799350	4	1	theme	Alginate-IFPG	892:904	arg1	beads					906:910	the Alginate-IFPG beads	888:910	the Alginate-IFPG beads	888:910	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads are due to hydrogen bonding and electrostatic interactions.					
36799350	3	2	theme	beads	710:714	arg1	5.76 mm					699:705	5.76 mm	699:705	5.76 mm	699:705	The Al-IFPG, Al-IFPG/WPI and Al-IFPG/PPC beads revealed 5.66, 6.85 and 5.76 mm of beads size, respectively and almost all of them engrossed a homogeneous and spherical structure.					
36799350	3	2	theme	beads	710:714	arg1	beads					710:714	beads	710:714	beads	710:714	The Al-IFPG, Al-IFPG/WPI and Al-IFPG/PPC beads revealed 5.66, 6.85 and 5.76 mm of beads size, respectively and almost all of them engrossed a homogeneous and spherical structure.					
36799350	2	3	theme	encapsulation	502:514	arg1	efficiency					516:525	the highest encapsulation efficiency	490:525	the highest encapsulation efficiency (10.87%)	490:534	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	2	3	theme	encapsulation	502:514	arg1	%					533:533	10.87%	528:533	10.87%	528:533	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	2	4	theme	mg/l	566:569	arg1	acid					578:581	120.8 mg/l gallic acid	560:581	120.8 mg/l gallic acid	560:581	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	2	4	theme	mg/l	566:569	arg1	content					551:557	polyphenol content	540:557	polyphenol content (120.8 mg/l gallic acid)	540:582	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	6	5	theme	beads	1209:1213	arg1	hardness					1197:1204	the hardness	1193:1204	the hardness of beads	1193:1213	The texture evaluation of JE-loaded beads based on Al-IFPG incorporation with WPI revealed increment the hardness of beads.					
36799350	1	6	theme	hydrocolloid	377:388	arg1	fraction					400:407	hydrocolloid insoluble fraction	377:407	hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al)	377:454	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	0	7	dep	Na-alginate/IFPG	120:135	arg1	fraction					148:155	insoluble fraction	138:155	insoluble fraction of Persian gum	138:170	Production and characterization of Ziziphus jujuba extract-loaded composite whey protein and pea protein beads based on Na-alginate/IFPG (insoluble fraction of Persian gum).					
36799350	1	8	theme	chickpea	308:315	arg1	concentrate					325:335	chickpea protein concentrate	308:335	chickpea protein concentrate (PPC)	308:341	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	1	8	theme	chickpea	308:315	arg1	PPC					338:340	PPC	338:340	PPC	338:340	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	7	9	theme	Al-IFPG	1285:1291	arg1	beads					1301:1305	Al-IFPG complex beads	1285:1305	Al-IFPG complex beads	1285:1305	CONCLUSION Thus, the outcomes of this study confirm the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins and expand its use in products.					
36799350	1	10	theme	insoluble	390:398	arg1	fraction					400:407	hydrocolloid insoluble fraction	377:407	hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al)	377:454	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	1	11	theme	-loaded	246:252	arg1	beads					254:258	jujube extract (JE)-loaded beads	227:258	jujube extract (JE)-loaded beads	227:258	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	0	12	theme	protein	97:103	arg1	beads					105:109	pea protein beads	93:109	pea protein beads	93:109	Production and characterization of Ziziphus jujuba extract-loaded composite whey protein and pea protein beads based on Na-alginate/IFPG (insoluble fraction of Persian gum).					
36799350	1	13	theme	protein	317:323	arg1	concentrate					325:335	chickpea protein concentrate	308:335	chickpea protein concentrate (PPC)	308:341	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	1	13	theme	protein	317:323	arg1	PPC					338:340	PPC	338:340	PPC	338:340	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	1	14	theme	fraction	400:407	arg1	types					368:372	two types	364:372	two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al)	364:454	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	6	15	theme	texture	1096:1102	arg1	evaluation					1104:1113	The texture evaluation	1092:1113	The texture evaluation of JE-loaded beads based on Al-IFPG incorporation with WPI	1092:1172	The texture evaluation of JE-loaded beads based on Al-IFPG incorporation with WPI revealed increment the hardness of beads.					
36799350	1	16	theme	beads	254:258	arg1	fabrication					212:222	the fabrication	208:222	the fabrication of jujube extract (JE)-loaded beads by extrusion	208:271	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	4	17	theme	stable	867:872	arg1	structures					874:883	the stable structures	863:883	the stable structures of the Alginate-IFPG beads	863:910	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads are due to hydrogen bonding and electrostatic interactions.					
36799350	7	18	theme	whey	1383:1386	arg1	proteins					1388:1395	whey proteins	1383:1395	whey proteins	1383:1395	CONCLUSION Thus, the outcomes of this study confirm the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins and expand its use in products.					
36799350	3	19	theme	homogeneous	770:780	arg1	structure					796:804	a homogeneous and spherical structure	768:804	a homogeneous and spherical structure	768:804	The Al-IFPG, Al-IFPG/WPI and Al-IFPG/PPC beads revealed 5.66, 6.85 and 5.76 mm of beads size, respectively and almost all of them engrossed a homogeneous and spherical structure.					
36799350	7	20	theme	jujube	1337:1342	arg1	extract					1344:1350	jujube extract	1337:1350	jujube extract	1337:1350	CONCLUSION Thus, the outcomes of this study confirm the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins and expand its use in products.					
36799350	1	21	dep	alginate	442:449	arg1	Al					452:453	Al	452:453	Al	452:453	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	2	22	theme	120.8	560:564	arg1	mg/l					566:569	mg/l	566:569	mg/l	566:569	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	3	23	dep	5.66	684:687	arg1	size					716:719	size	716:719	size	716:719	The Al-IFPG, Al-IFPG/WPI and Al-IFPG/PPC beads revealed 5.66, 6.85 and 5.76 mm of beads size, respectively and almost all of them engrossed a homogeneous and spherical structure.					
36799350	2	24	theme	polyphenol	540:549	arg1	acid					578:581	120.8 mg/l gallic acid	560:581	120.8 mg/l gallic acid	560:581	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	2	24	theme	polyphenol	540:549	arg1	content					551:557	polyphenol content	540:557	polyphenol content (120.8 mg/l gallic acid)	540:582	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	1	25	theme	Persian	412:418	arg1	gum					420:422	Persian gum	412:422	Persian gum (IFPG)	412:429	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	1	25	theme	Persian	412:418	arg1	IFPG					425:428	IFPG	425:428	IFPG	425:428	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	7	26	theme	extract	1344:1350	arg1	delivery					1325:1332	the effective delivery	1311:1332	the effective delivery of jujube extract via incorporation into pea and whey proteins	1311:1395	CONCLUSION Thus, the outcomes of this study confirm the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins and expand its use in products.					
36799350	7	27	theme	study	1254:1258	arg1	outcomes					1237:1244	the outcomes	1233:1244	the outcomes of this study	1233:1258	CONCLUSION Thus, the outcomes of this study confirm the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins and expand its use in products.					
36799350	1	28	theme	gum	420:422	arg1	fraction					400:407	hydrocolloid insoluble fraction	377:407	hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al)	377:454	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	3	29	theme	spherical	786:794	arg1	structure					796:804	a homogeneous and spherical structure	768:804	a homogeneous and spherical structure	768:804	The Al-IFPG, Al-IFPG/WPI and Al-IFPG/PPC beads revealed 5.66, 6.85 and 5.76 mm of beads size, respectively and almost all of them engrossed a homogeneous and spherical structure.					
36799350	4	30	theme	hydrogen	923:930	arg1	bonding					932:938	hydrogen bonding	923:938	hydrogen bonding	923:938	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads are due to hydrogen bonding and electrostatic interactions.					
36799350	4	31	theme	electrostatic	944:956	arg1	interactions					958:969	electrostatic interactions	944:969	electrostatic interactions	944:969	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads are due to hydrogen bonding and electrostatic interactions.					
36799350	4	32	theme	Fourier-transform	811:827	arg1	due					916:918	due	916:918	due	916:918	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads are due to hydrogen bonding and electrostatic interactions.					
36799350	4	32	theme	Fourier-transform	811:827	arg1	data					851:854	The Fourier-transform infrared spectroscopy data	807:854	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads	807:910	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads are due to hydrogen bonding and electrostatic interactions.					
36799350	7	33	theme	complex	1293:1299	arg1	beads					1301:1305	Al-IFPG complex beads	1285:1305	Al-IFPG complex beads	1285:1305	CONCLUSION Thus, the outcomes of this study confirm the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins and expand its use in products.					
36799350	2	34	theme	gallic	571:576	arg1	acid					578:581	120.8 mg/l gallic acid	560:581	120.8 mg/l gallic acid	560:581	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	2	34	theme	gallic	571:576	arg1	content					551:557	polyphenol content	540:557	polyphenol content (120.8 mg/l gallic acid)	540:582	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	2	35	theme	JE-loaded	469:477	arg1	beads					479:483	The JE-loaded beads	465:483	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid)	457:582	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	7	36	theme	effective	1315:1323	arg1	delivery					1325:1332	the effective delivery	1311:1332	the effective delivery of jujube extract via incorporation into pea and whey proteins	1311:1395	CONCLUSION Thus, the outcomes of this study confirm the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins and expand its use in products.					
36799350	0	37	theme	jujuba	44:49	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of Ziziphus jujuba extract-loaded composite whey protein and pea protein beads based on Na-alginate/IFPG (insoluble fraction of Persian gum).					
36799350	0	37	theme	jujuba	44:49	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of Ziziphus jujuba extract-loaded composite whey protein and pea protein beads based on Na-alginate/IFPG (insoluble fraction of Persian gum).					
36799350	4	38	theme	spectroscopy	838:849	arg1	due					916:918	due	916:918	due	916:918	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads are due to hydrogen bonding and electrostatic interactions.					
36799350	4	38	theme	spectroscopy	838:849	arg1	data					851:854	The Fourier-transform infrared spectroscopy data	807:854	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads	807:910	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads are due to hydrogen bonding and electrostatic interactions.					
36799350	5	39	theme	beads	995:999	arg1	thermostability					976:990	The thermostability	972:990	The thermostability of beads loaded with JE based on Al-IFPG/WPI	972:1035	The thermostability of beads loaded with JE based on Al-IFPG/WPI were significantly enhanced, compared to pure Al-IFPG.					
36799350	7	40	theme	beads	1301:1305	arg1	potential					1272:1280	the potential	1268:1280	the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins	1268:1395	CONCLUSION Thus, the outcomes of this study confirm the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins and expand its use in products.					
36799350	4	41	theme	beads	906:910	arg1	structures					874:883	the stable structures	863:883	the stable structures of the Alginate-IFPG beads	863:910	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads are due to hydrogen bonding and electrostatic interactions.					
36799350	1	42	theme	whey	280:283	arg1	WPI					302:304	WPI	302:304	WPI	302:304	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	1	42	theme	whey	280:283	arg1	isolate					293:299	whey protein isolate	280:299	whey protein isolate (WPI)	280:305	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	0	43	theme	insoluble	138:146	arg1	fraction					148:155	insoluble fraction	138:155	insoluble fraction of Persian gum	138:170	Production and characterization of Ziziphus jujuba extract-loaded composite whey protein and pea protein beads based on Na-alginate/IFPG (insoluble fraction of Persian gum).					
36799350	0	44	theme	composite	66:74	arg1	protein					81:87	composite whey protein	66:87	composite whey protein	66:87	Production and characterization of Ziziphus jujuba extract-loaded composite whey protein and pea protein beads based on Na-alginate/IFPG (insoluble fraction of Persian gum).					
36799350	1	45	theme	protein	285:291	arg1	WPI					302:304	WPI	302:304	WPI	302:304	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	1	45	theme	protein	285:291	arg1	isolate					293:299	whey protein isolate	280:299	whey protein isolate (WPI)	280:305	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	0	46	theme	Persian	160:166	arg1	gum					168:170	Persian gum	160:170	Persian gum	160:170	Production and characterization of Ziziphus jujuba extract-loaded composite whey protein and pea protein beads based on Na-alginate/IFPG (insoluble fraction of Persian gum).					
36799350	6	47	theme	Al-IFPG	1143:1149	arg1	incorporation					1151:1163	Al-IFPG incorporation	1143:1163	Al-IFPG incorporation with WPI	1143:1172	The texture evaluation of JE-loaded beads based on Al-IFPG incorporation with WPI revealed increment the hardness of beads.					
36799350	4	48	theme	infrared	829:836	arg1	due					916:918	due	916:918	due	916:918	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads are due to hydrogen bonding and electrostatic interactions.					
36799350	4	48	theme	infrared	829:836	arg1	data					851:854	The Fourier-transform infrared spectroscopy data	807:854	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads	807:910	The Fourier-transform infrared spectroscopy data proved the stable structures of the Alginate-IFPG beads are due to hydrogen bonding and electrostatic interactions.					
36799350	5	49	theme	pure	1078:1081	arg1	Al-IFPG					1083:1089	pure Al-IFPG	1078:1089	pure Al-IFPG	1078:1089	The thermostability of beads loaded with JE based on Al-IFPG/WPI were significantly enhanced, compared to pure Al-IFPG.					
36799350	2	50	theme	highest	494:500	arg1	efficiency					516:525	the highest encapsulation efficiency	490:525	the highest encapsulation efficiency (10.87%)	490:534	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	2	50	theme	highest	494:500	arg1	%					533:533	10.87%	528:533	10.87%	528:533	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	1	51	dep	BACKGROUND	174:183	arg1	aimed					199:203	aimed	199:203	aimed	199:203	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	1	52	theme	sodium	435:440	arg1	alginate					442:449	sodium alginate	435:449	sodium alginate (Al)	435:454	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	3	53	dep	Al-IFPG	632:638	arg1	beads					669:673	beads	669:673	beads	669:673	The Al-IFPG, Al-IFPG/WPI and Al-IFPG/PPC beads revealed 5.66, 6.85 and 5.76 mm of beads size, respectively and almost all of them engrossed a homogeneous and spherical structure.					
36799350	7	54	dep	CONCLUSION	1216:1225	arg1	confirm					1260:1266	confirm	1260:1266	confirm the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins	1260:1395	CONCLUSION Thus, the outcomes of this study confirm the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins and expand its use in products.					
36799350	7	54	dep	CONCLUSION	1216:1225	arg1	expand					1401:1406	expand	1401:1406	expand its use in products	1401:1426	CONCLUSION Thus, the outcomes of this study confirm the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins and expand its use in products.					
36799350	1	55	theme	alginate	442:449	arg1	fraction					400:407	hydrocolloid insoluble fraction	377:407	hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al)	377:454	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	0	56	theme	gum	168:170	arg1	fraction					148:155	insoluble fraction	138:155	insoluble fraction of Persian gum	138:170	Production and characterization of Ziziphus jujuba extract-loaded composite whey protein and pea protein beads based on Na-alginate/IFPG (insoluble fraction of Persian gum).					
36799350	1	57	theme	types	368:372	arg1	concentrate					325:335	chickpea protein concentrate	308:335	chickpea protein concentrate (PPC)	308:341	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	1	57	theme	types	368:372	arg1	WPI					302:304	WPI	302:304	WPI	302:304	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	1	57	theme	types	368:372	arg1	combination					349:359	a combination	347:359	a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al)	347:454	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	1	57	theme	types	368:372	arg1	isolate					293:299	whey protein isolate	280:299	whey protein isolate (WPI)	280:305	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	1	57	theme	types	368:372	arg1	PPC					338:340	PPC	338:340	PPC	338:340	BACKGROUND This research aimed at the fabrication of jujube extract (JE)-loaded beads by extrusion, using whey protein isolate (WPI), chickpea protein concentrate (PPC) and a combination of two types of hydrocolloid insoluble fraction of Persian gum (IFPG) and sodium alginate (Al).					
36799350	6	58	theme	beads	1128:1132	arg1	evaluation					1104:1113	The texture evaluation	1092:1113	The texture evaluation of JE-loaded beads based on Al-IFPG incorporation with WPI	1092:1172	The texture evaluation of JE-loaded beads based on Al-IFPG incorporation with WPI revealed increment the hardness of beads.					
36799350	2	59	with	beads	479:483	arg1	efficiency					516:525	the highest encapsulation efficiency	490:525	the highest encapsulation efficiency (10.87%)	490:534	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	2	59	with	beads	479:483	arg1	%					533:533	10.87%	528:533	10.87%	528:533	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	2	59	with	beads	479:483	arg1	acid					578:581	120.8 mg/l gallic acid	560:581	120.8 mg/l gallic acid	560:581	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	2	59	with	beads	479:483	arg1	content					551:557	polyphenol content	540:557	polyphenol content (120.8 mg/l gallic acid)	540:582	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36799350	7	60	from	use	1412:1414	arg1	products					1419:1426	products	1419:1426	products	1419:1426	CONCLUSION Thus, the outcomes of this study confirm the potential of Al-IFPG complex beads for the effective delivery of jujube extract via incorporation into pea and whey proteins and expand its use in products.					
36799350	6	61	theme	JE-loaded	1118:1126	arg1	beads					1128:1132	JE-loaded beads	1118:1132	JE-loaded beads based on Al-IFPG incorporation with WPI	1118:1172	The texture evaluation of JE-loaded beads based on Al-IFPG incorporation with WPI revealed increment the hardness of beads.					
36799350	0	62	theme	whey	76:79	arg1	protein					81:87	composite whey protein	66:87	composite whey protein	66:87	Production and characterization of Ziziphus jujuba extract-loaded composite whey protein and pea protein beads based on Na-alginate/IFPG (insoluble fraction of Persian gum).					
36799350	6	63	with	incorporation	1151:1163	arg1	WPI					1170:1172	WPI	1170:1172	WPI	1170:1172	The texture evaluation of JE-loaded beads based on Al-IFPG incorporation with WPI revealed increment the hardness of beads.					
36799350	2	64	dep	RESULTS	457:463	arg1	beads					479:483	The JE-loaded beads	465:483	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid)	457:582	RESULTS The JE-loaded beads with the highest encapsulation efficiency (10.87%) and polyphenol content (120.8 mg/l gallic acid) were obtained by Alginate-IFPG/PPC at 4 °C.					
36499399	1	0	theme	polymeric	140:148	arg1	catheters					166:174	polymeric urinary medical catheters	140:174	polymeric urinary medical catheters	140:174	A method for the immobilization of an antibacterial chitosan coating to polymeric urinary medical catheters is presented.					
36499399	1	1	theme	urinary	150:156	arg1	catheters					166:174	polymeric urinary medical catheters	140:174	polymeric urinary medical catheters	140:174	A method for the immobilization of an antibacterial chitosan coating to polymeric urinary medical catheters is presented.					
36499399	4	2	theme	polymeric	541:549	arg1	catheters					551:559	polymeric catheters	541:559	polymeric catheters	541:559	In the second plasma step, polymeric catheters are treated with atomic oxygen to form oxygen-containing surface functional groups acting as binding sites for chitosan.					
36499399	5	3	theme	functional	705:714	arg1	groups					716:721	oxygen functional groups	698:721	oxygen functional groups	698:721	The presence of oxygen functional groups also causes a transformation of the hydrophobic polymer surface to hydrophilic, thus enabling uniform wetting and improved adsorption of the chitosan coating.					
36499399	5	4	theme	surface	779:785	arg1	transformation					737:750	a transformation	735:750	a transformation of the hydrophobic polymer surface to hydrophilic	735:800	The presence of oxygen functional groups also causes a transformation of the hydrophobic polymer surface to hydrophilic, thus enabling uniform wetting and improved adsorption of the chitosan coating.					
36499399	2	5	from	solution	304:311	arg1	deposition					266:275	the deposition	262:275	the deposition of chitosan from the water solution	262:311	The method comprises a two-step plasma-treatment procedure, followed by the deposition of chitosan from the water solution.					
36499399	3	6	theme	urinary	344:350	arg1	catheter					352:359	the urinary catheter	340:359	the urinary catheter	340:359	In the first plasma step, the urinary catheter is treated with vacuum-ultraviolet radiation to break bonds in the polymer surface film and create dangling bonds, which are occupied by hydrogen atoms.					
36499399	2	7	theme	chitosan	280:287	arg1	deposition					266:275	the deposition	262:275	the deposition of chitosan from the water solution	262:311	The method comprises a two-step plasma-treatment procedure, followed by the deposition of chitosan from the water solution.					
36499399	8	8	theme	plasma	1164:1169	arg1	treatment					1171:1179	plasma treatment	1164:1179	plasma treatment	1164:1179	The effect of plasma treatment on immobilization was explained by noncovalent interactions such as electrostatic interactions and hydrogen bonds.					
36499399	8	9	theme	noncovalent	1216:1226	arg1	interactions					1228:1239	noncovalent interactions	1216:1239	noncovalent interactions such as electrostatic interactions and hydrogen bonds	1216:1293	The effect of plasma treatment on immobilization was explained by noncovalent interactions such as electrostatic interactions and hydrogen bonds.					
36499399	8	9	theme	noncovalent	1216:1226	arg1	interactions					1263:1274	electrostatic interactions	1249:1274	electrostatic interactions	1249:1274	The effect of plasma treatment on immobilization was explained by noncovalent interactions such as electrostatic interactions and hydrogen bonds.					
36499399	8	9	theme	noncovalent	1216:1226	arg1	bonds					1289:1293	hydrogen bonds	1280:1293	hydrogen bonds	1280:1293	The effect of plasma treatment on immobilization was explained by noncovalent interactions such as electrostatic interactions and hydrogen bonds.					
36499399	7	10	theme	chitosan	1125:1132	arg1	immobilization					1134:1147	successful chitosan immobilization	1114:1147	successful chitosan immobilization	1114:1147	Non-treated samples did not exhibit successful chitosan immobilization.					
36499399	1	11	theme	medical	158:164	arg1	catheters					166:174	polymeric urinary medical catheters	140:174	polymeric urinary medical catheters	140:174	A method for the immobilization of an antibacterial chitosan coating to polymeric urinary medical catheters is presented.					
36499399	8	12	theme	hydrogen	1280:1287	arg1	bonds					1289:1293	hydrogen bonds	1280:1293	hydrogen bonds	1280:1293	The effect of plasma treatment on immobilization was explained by noncovalent interactions such as electrostatic interactions and hydrogen bonds.					
36499399	3	13	theme	polymer	428:434	arg1	film					444:447	the polymer surface film	424:447	the polymer surface film	424:447	In the first plasma step, the urinary catheter is treated with vacuum-ultraviolet radiation to break bonds in the polymer surface film and create dangling bonds, which are occupied by hydrogen atoms.					
36499399	8	14	theme	treatment	1171:1179	arg1	effect					1154:1159	The effect	1150:1159	The effect of plasma treatment on immobilization	1150:1197	The effect of plasma treatment on immobilization was explained by noncovalent interactions such as electrostatic interactions and hydrogen bonds.					
36499399	4	15	theme	binding	654:660	arg1	sites					662:666	binding sites	654:666	binding sites for chitosan	654:679	In the second plasma step, polymeric catheters are treated with atomic oxygen to form oxygen-containing surface functional groups acting as binding sites for chitosan.					
36499399	4	16	theme	plasma	528:533	arg1	step					535:538	the second plasma step	517:538	the second plasma step	517:538	In the second plasma step, polymeric catheters are treated with atomic oxygen to form oxygen-containing surface functional groups acting as binding sites for chitosan.					
36499399	7	17	theme	successful	1114:1123	arg1	immobilization					1134:1147	successful chitosan immobilization	1114:1147	successful chitosan immobilization	1114:1147	Non-treated samples did not exhibit successful chitosan immobilization.					
36499399	6	18	theme	infrared	1055:1062	arg1	spectroscopy					1064:1075	Fourier-transform infrared spectroscopy	1037:1075	Fourier-transform infrared spectroscopy	1037:1075	The wettability was measured by the sessile-drop method, while the surface composition and structure were measured by X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy.					
36499399	5	19	theme	chitosan	864:871	arg1	coating					873:879	the chitosan coating	860:879	the chitosan coating	860:879	The presence of oxygen functional groups also causes a transformation of the hydrophobic polymer surface to hydrophilic, thus enabling uniform wetting and improved adsorption of the chitosan coating.					
36499399	8	20	theme	electrostatic	1249:1261	arg1	interactions					1263:1274	electrostatic interactions	1249:1274	electrostatic interactions	1249:1274	The effect of plasma treatment on immobilization was explained by noncovalent interactions such as electrostatic interactions and hydrogen bonds.					
36499399	4	21	theme	second	521:526	arg1	step					535:538	the second plasma step	517:538	the second plasma step	517:538	In the second plasma step, polymeric catheters are treated with atomic oxygen to form oxygen-containing surface functional groups acting as binding sites for chitosan.					
36499399	2	22	theme	water	298:302	arg1	solution					304:311	the water solution	294:311	the water solution	294:311	The method comprises a two-step plasma-treatment procedure, followed by the deposition of chitosan from the water solution.					
36499399	4	23	theme	oxygen-containing	600:616	arg1	groups					637:642	oxygen-containing surface functional groups	600:642	oxygen-containing surface functional groups acting as binding sites for chitosan	600:679	In the second plasma step, polymeric catheters are treated with atomic oxygen to form oxygen-containing surface functional groups acting as binding sites for chitosan.					
36499399	6	24	theme	surface	949:955	arg1	composition					957:967	the surface composition	945:967	the surface composition	945:967	The wettability was measured by the sessile-drop method, while the surface composition and structure were measured by X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy.					
36499399	5	25	theme	wetting	825:831	arg1	uniform					817:823	uniform wetting	817:831	uniform wetting	817:831	The presence of oxygen functional groups also causes a transformation of the hydrophobic polymer surface to hydrophilic, thus enabling uniform wetting and improved adsorption of the chitosan coating.					
36499399	5	26	theme	hydrophobic	759:769	arg1	surface					779:785	the hydrophobic polymer surface	755:785	the hydrophobic polymer surface	755:785	The presence of oxygen functional groups also causes a transformation of the hydrophobic polymer surface to hydrophilic, thus enabling uniform wetting and improved adsorption of the chitosan coating.					
36499399	7	27	theme	Non-treated	1078:1088	arg1	samples					1090:1096	Non-treated samples	1078:1096	Non-treated samples	1078:1096	Non-treated samples did not exhibit successful chitosan immobilization.					
36499399	4	28	theme	functional	626:635	arg1	groups					637:642	oxygen-containing surface functional groups	600:642	oxygen-containing surface functional groups acting as binding sites for chitosan	600:679	In the second plasma step, polymeric catheters are treated with atomic oxygen to form oxygen-containing surface functional groups acting as binding sites for chitosan.					
36499399	3	29	theme	surface	436:442	arg1	film					444:447	the polymer surface film	424:447	the polymer surface film	424:447	In the first plasma step, the urinary catheter is treated with vacuum-ultraviolet radiation to break bonds in the polymer surface film and create dangling bonds, which are occupied by hydrogen atoms.					
36499399	5	30	theme	polymer	771:777	arg1	surface					779:785	the hydrophobic polymer surface	755:785	the hydrophobic polymer surface	755:785	The presence of oxygen functional groups also causes a transformation of the hydrophobic polymer surface to hydrophilic, thus enabling uniform wetting and improved adsorption of the chitosan coating.					
36499399	5	31	theme	oxygen	698:703	arg1	groups					716:721	oxygen functional groups	698:721	oxygen functional groups	698:721	The presence of oxygen functional groups also causes a transformation of the hydrophobic polymer surface to hydrophilic, thus enabling uniform wetting and improved adsorption of the chitosan coating.					
36499399	8	32	from	effect	1154:1159	arg1	immobilization					1184:1197	immobilization	1184:1197	immobilization	1184:1197	The effect of plasma treatment on immobilization was explained by noncovalent interactions such as electrostatic interactions and hydrogen bonds.					
36499399	2	33	theme	plasma-treatment	222:237	arg1	procedure					239:247	a two-step plasma-treatment procedure	211:247	a two-step plasma-treatment procedure	211:247	The method comprises a two-step plasma-treatment procedure, followed by the deposition of chitosan from the water solution.					
36499399	4	34	theme	surface	618:624	arg1	groups					637:642	oxygen-containing surface functional groups	600:642	oxygen-containing surface functional groups acting as binding sites for chitosan	600:679	In the second plasma step, polymeric catheters are treated with atomic oxygen to form oxygen-containing surface functional groups acting as binding sites for chitosan.					
36499399	3	35	theme	vacuum-ultraviolet	377:394	arg1	radiation					396:404	vacuum-ultraviolet radiation	377:404	vacuum-ultraviolet radiation	377:404	In the first plasma step, the urinary catheter is treated with vacuum-ultraviolet radiation to break bonds in the polymer surface film and create dangling bonds, which are occupied by hydrogen atoms.					
36499399	6	36	theme	Fourier-transform	1037:1053	arg1	spectroscopy					1064:1075	Fourier-transform infrared spectroscopy	1037:1075	Fourier-transform infrared spectroscopy	1037:1075	The wettability was measured by the sessile-drop method, while the surface composition and structure were measured by X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy.					
36499399	3	37	theme	plasma	327:332	arg1	step					334:337	the first plasma step	317:337	the first plasma step	317:337	In the first plasma step, the urinary catheter is treated with vacuum-ultraviolet radiation to break bonds in the polymer surface film and create dangling bonds, which are occupied by hydrogen atoms.					
36499399	2	38	theme	two-step	213:220	arg1	procedure					239:247	a two-step plasma-treatment procedure	211:247	a two-step plasma-treatment procedure	211:247	The method comprises a two-step plasma-treatment procedure, followed by the deposition of chitosan from the water solution.					
36499399	0	39	theme	Chitosan	35:42	arg1	Immobilization					17:30	the Immobilization	13:30	the Immobilization of Chitosan onto Urinary Catheters	13:65	A Method for the Immobilization of Chitosan onto Urinary Catheters.					
36499399	6	40	theme	sessile-drop	918:929	arg1	method					931:936	the sessile-drop method	914:936	the sessile-drop method	914:936	The wettability was measured by the sessile-drop method, while the surface composition and structure were measured by X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy.					
36499399	3	41	used	occupied	486:493	arg2	bonds					469:473	dangling bonds	460:473	dangling bonds	460:473	In the first plasma step, the urinary catheter is treated with vacuum-ultraviolet radiation to break bonds in the polymer surface film and create dangling bonds, which are occupied by hydrogen atoms.					
36499399	1	42	theme	antibacterial	106:118	arg1	chitosan					120:127	an antibacterial chitosan	103:127	an antibacterial chitosan coating to polymeric urinary medical catheters	103:174	A method for the immobilization of an antibacterial chitosan coating to polymeric urinary medical catheters is presented.					
36499399	4	43	theme	atomic	578:583	arg1	oxygen					585:590	atomic oxygen	578:590	atomic oxygen	578:590	In the second plasma step, polymeric catheters are treated with atomic oxygen to form oxygen-containing surface functional groups acting as binding sites for chitosan.					
36499399	3	44	from	bonds	415:419	arg1	film					444:447	the polymer surface film	424:447	the polymer surface film	424:447	In the first plasma step, the urinary catheter is treated with vacuum-ultraviolet radiation to break bonds in the polymer surface film and create dangling bonds, which are occupied by hydrogen atoms.					
36499399	1	45	theme	chitosan	120:127	arg1	immobilization					85:98	the immobilization	81:98	the immobilization of an antibacterial chitosan coating to polymeric urinary medical catheters	81:174	A method for the immobilization of an antibacterial chitosan coating to polymeric urinary medical catheters is presented.					
36499399	3	46	theme	dangling	460:467	arg1	bonds					469:473	dangling bonds	460:473	dangling bonds	460:473	In the first plasma step, the urinary catheter is treated with vacuum-ultraviolet radiation to break bonds in the polymer surface film and create dangling bonds, which are occupied by hydrogen atoms.					
36499399	3	47	theme	first	321:325	arg1	step					334:337	the first plasma step	317:337	the first plasma step	317:337	In the first plasma step, the urinary catheter is treated with vacuum-ultraviolet radiation to break bonds in the polymer surface film and create dangling bonds, which are occupied by hydrogen atoms.					
36499399	5	48	theme	improved	837:844	arg1	adsorption					846:855	improved adsorption	837:855	improved adsorption of the chitosan coating	837:879	The presence of oxygen functional groups also causes a transformation of the hydrophobic polymer surface to hydrophilic, thus enabling uniform wetting and improved adsorption of the chitosan coating.					
36499399	5	49	theme	coating	873:879	arg1	uniform					817:823	uniform wetting	817:831	uniform wetting	817:831	The presence of oxygen functional groups also causes a transformation of the hydrophobic polymer surface to hydrophilic, thus enabling uniform wetting and improved adsorption of the chitosan coating.					
36499399	5	49	theme	coating	873:879	arg1	adsorption					846:855	improved adsorption	837:855	improved adsorption of the chitosan coating	837:879	The presence of oxygen functional groups also causes a transformation of the hydrophobic polymer surface to hydrophilic, thus enabling uniform wetting and improved adsorption of the chitosan coating.					
36499399	6	50	theme	photoelectron	1006:1018	arg1	spectroscopy					1020:1031	X-ray photoelectron spectroscopy	1000:1031	X-ray photoelectron spectroscopy	1000:1031	The wettability was measured by the sessile-drop method, while the surface composition and structure were measured by X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy.					
36499399	0	51	theme	Urinary	49:55	arg1	Catheters					57:65	Urinary Catheters	49:65	Urinary Catheters	49:65	A Method for the Immobilization of Chitosan onto Urinary Catheters.					
36499399	6	52	theme	X-ray	1000:1004	arg1	spectroscopy					1020:1031	X-ray photoelectron spectroscopy	1000:1031	X-ray photoelectron spectroscopy	1000:1031	The wettability was measured by the sessile-drop method, while the surface composition and structure were measured by X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy.					
36499399	3	53	theme	hydrogen	498:505	arg1	atoms					507:511	hydrogen atoms	498:511	hydrogen atoms	498:511	In the first plasma step, the urinary catheter is treated with vacuum-ultraviolet radiation to break bonds in the polymer surface film and create dangling bonds, which are occupied by hydrogen atoms.					
36499399	5	54	theme	groups	716:721	arg1	presence					686:693	The presence	682:693	The presence of oxygen functional groups	682:721	The presence of oxygen functional groups also causes a transformation of the hydrophobic polymer surface to hydrophilic, thus enabling uniform wetting and improved adsorption of the chitosan coating.					
35176297	6	0	theme	0.994	1031:1035	arg1	R2					1014:1015	R2	1014:1015	R2	1014:1015	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	6	0	theme	0.994	1031:1035	arg1	Adj.R2					1021:1026	Adj.R2	1021:1026	Adj.R2	1021:1026	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	11	1	theme	pseudo-second	1556:1568	arg1	model					1582:1586	the pseudo-second order (PSO) model	1552:1586	the pseudo-second order (PSO) model	1552:1586	The kinetic adsorption data for WSA and WSA/starch/Fe3O4 followed the pseudo-second order (PSO) model.					
35176297	2	2	theme	desired	308:314	arg1	nanocomposites					316:329	The desired nanocomposites	304:329	The desired nanocomposites	304:329	The desired nanocomposites were successfully synthesized by the chemical deposition method.					
35176297	1	3	theme	WSA	236:238	arg1	/starch/Fe3O4					240:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	5	4	dep	success	749:755	arg1	estimate					805:812	estimate	805:812	to estimate the appropriate response	802:837	This method showed the success of the model to design process variables and to estimate the appropriate response.					
35176297	5	4	dep	success	749:755	arg1	design					773:778	design	773:778	to design process variables	770:796	This method showed the success of the model to design process variables and to estimate the appropriate response.					
35176297	4	5	theme	response	580:587	arg1	method					597:602	the response surface method	576:602	the response surface method	576:602	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	9	6	contain	had	1309:1311	arg1	energy					1293:1298	The free energy	1284:1298	The free energy of Gibbs	1284:1307	The free energy of Gibbs had a negative value at 25-45 °C indicating that the adsorption process is spontaneous.					
35176297	9	6	contain	had	1309:1311	arg2	value					1324:1328	a negative value	1313:1328	a negative value at 25-45 °C	1313:1340	The free energy of Gibbs had a negative value at 25-45 °C indicating that the adsorption process is spontaneous.					
35176297	1	7	theme	/starch/Fe3O4	240:252	arg1	/starch/Fe3O4					240:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	1	7	theme	/starch/Fe3O4	240:252	arg1	nanocomposite					201:213	a new magnetic nanocomposite	186:213	a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples	186:301	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	4	8	theme	pH	643:644	arg1	influence					630:638	the influence	626:638	the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time	626:723	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	4	9	theme	adsorbent	647:655	arg1	quantity					657:664	adsorbent quantity	647:664	adsorbent quantity	647:664	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	12	10	theme	groundwater	1656:1666	arg1	samples					1668:1674	three groundwater samples	1650:1674	three groundwater samples	1650:1674	The ability of the composite adsorbent to remove copper from three groundwater samples showed that it could be reused at least 3 times with appropriate efficiency, depending on the water quality.					
35176297	8	11	theme	sorption	1187:1194	arg1	capacity					1196:1203	The highest Cu (II) sorption capacity	1167:1203	The highest Cu (II) sorption capacity of 29.0 and 45.4 mg/g	1167:1225	The highest Cu (II) sorption capacity of 29.0 and 45.4 mg/g was attained for WSA and WSA/starch/Fe3O4, respectively.					
35176297	4	12	used	utilized	608:615	arg2	design					565:570	A central composite design	545:570	A central composite design for the response surface method	545:602	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	1	13	attach	present	272:278	arg2	II					268:269	II	268:269	II	268:269	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	1	13	attach	present	272:278	arg1	samples					295:301	groundwater samples	283:301	groundwater samples	283:301	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	1	13	attach	present	272:278	arg2	Cu					264:265	Cu	264:265	Cu (II) present in groundwater samples	264:301	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	1	14	theme	new	188:190	arg1	/starch/Fe3O4					240:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	1	14	theme	new	188:190	arg1	nanocomposite					201:213	a new magnetic nanocomposite	186:213	a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples	186:301	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	0	15	theme	samples	131:137	arg1	Treatment					106:114	Treatment	106:114	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.	0:138	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.					
35176297	6	16	theme	quadratic	878:886	arg1	model					899:903	the quadratic polynomial model	874:903	the quadratic polynomial model	874:903	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	4	17	theme	Cu	686:687	arg1	temperature					695:705	temperature	695:705	temperature	695:705	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	4	17	theme	Cu	686:687	arg1	pH					643:644	pH	643:644	pH	643:644	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	4	17	theme	Cu	686:687	arg1	quantity					657:664	adsorbent quantity	647:664	adsorbent quantity	647:664	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	4	17	theme	Cu	686:687	arg1	content					675:681	initial content	667:681	initial content of Cu (II)	667:692	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	4	17	theme	Cu	686:687	arg1	time					720:723	contact time	712:723	contact time	712:723	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	9	18	from	25-45 °C	1333:1340	arg1	value					1324:1328	a negative value	1313:1328	a negative value at 25-45 °C	1313:1340	The free energy of Gibbs had a negative value at 25-45 °C indicating that the adsorption process is spontaneous.					
35176297	0	19	theme	ash/starch/Fe3O4	54:69	arg1	adsorbent					28:36	adsorbent	28:36	adsorbent	28:36	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.					
35176297	1	20	theme	groundwater	283:293	arg1	samples					295:301	groundwater samples	283:301	groundwater samples	283:301	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	6	21	theme	proposed	949:956	arg1	model					958:962	the proposed model	945:962	the proposed model	945:962	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	4	22	theme	content	675:681	arg1	influence					630:638	the influence	626:638	the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time	626:723	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	0	23	theme	effective	75:83	arg1	ions					92:95	effective copper ions	75:95	effective copper ions	75:95	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.					
35176297	4	24	theme	contact	712:718	arg1	time					720:723	contact time	712:723	contact time	712:723	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	2	25	theme	chemical	368:375	arg1	method					388:393	the chemical deposition method	364:393	the chemical deposition method	364:393	The desired nanocomposites were successfully synthesized by the chemical deposition method.					
35176297	3	26	theme	specific	400:407	arg1	area					424:427	The specific active surface area	396:427	The specific active surface area for pristine WSA and WSA/starch/Fe3O4 magnetic nanocomposites	396:489	The specific active surface area for pristine WSA and WSA/starch/Fe3O4 magnetic nanocomposites was determined to be 8.1 and 52.6 m2/g, respectively.					
35176297	3	26	theme	specific	400:407	arg1	8.1					512:514	8.1	512:514	8.1	512:514	The specific active surface area for pristine WSA and WSA/starch/Fe3O4 magnetic nanocomposites was determined to be 8.1 and 52.6 m2/g, respectively.					
35176297	5	27	theme	model	764:768	arg1	success					749:755	the success	745:755	the success of the model to design process variables and to estimate the appropriate response	745:837	This method showed the success of the model to design process variables and to estimate the appropriate response.					
35176297	6	28	theme	predicted	994:1002	arg1	data					1004:1007	experimental and predicted data	977:1007	experimental and predicted data	977:1007	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	4	29	theme	central	547:553	arg1	design					565:570	A central composite design	545:570	A central composite design for the response surface method	545:602	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	10	30	theme	negative	1403:1410	arg1	values					1415:1420	negative ΔH values	1403:1420	negative ΔH values for copper adsorption	1403:1442	Also, negative ΔH values for copper adsorption showed that the processes are exothermic.					
35176297	8	31	theme	Cu	1179:1180	arg1	capacity					1196:1203	The highest Cu (II) sorption capacity	1167:1203	The highest Cu (II) sorption capacity of 29.0 and 45.4 mg/g	1167:1225	The highest Cu (II) sorption capacity of 29.0 and 45.4 mg/g was attained for WSA and WSA/starch/Fe3O4, respectively.					
35176297	0	32	theme	adsorbent	28:36	arg1	Development					0:10	Development	0:10	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.	0:138	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.					
35176297	6	33	theme	experimental	977:988	arg1	data					1004:1007	experimental and predicted data	977:1007	experimental and predicted data	977:1007	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	0	34	theme	walnut	41:46	arg1	ash/starch/Fe3O4					54:69	walnut shell ash/starch/Fe3O4	41:69	walnut shell ash/starch/Fe3O4	41:69	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.					
35176297	1	35	theme	present	272:278	arg1	Cu					264:265	Cu	264:265	Cu (II) present in groundwater samples	264:301	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	1	35	theme	present	272:278	arg1	II					268:269	II	268:269	II	268:269	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	12	36	theme	appropriate	1729:1739	arg1	efficiency					1741:1750	appropriate efficiency	1729:1750	appropriate efficiency	1729:1750	The ability of the composite adsorbent to remove copper from three groundwater samples showed that it could be reused at least 3 times with appropriate efficiency, depending on the water quality.					
35176297	10	37	theme	copper	1426:1431	arg1	adsorption					1433:1442	copper adsorption	1426:1442	copper adsorption	1426:1442	Also, negative ΔH values for copper adsorption showed that the processes are exothermic.					
35176297	3	38	theme	WSA/starch/Fe3O4	450:465	arg1	nanocomposites					476:489	WSA/starch/Fe3O4 magnetic nanocomposites	450:489	WSA/starch/Fe3O4 magnetic nanocomposites	450:489	The specific active surface area for pristine WSA and WSA/starch/Fe3O4 magnetic nanocomposites was determined to be 8.1 and 52.6 m2/g, respectively.					
35176297	9	39	theme	Gibbs	1303:1307	arg1	energy					1293:1298	The free energy	1284:1298	The free energy of Gibbs	1284:1307	The free energy of Gibbs had a negative value at 25-45 °C indicating that the adsorption process is spontaneous.					
35176297	4	40	theme	surface	589:595	arg1	method					597:602	the response surface method	576:602	the response surface method	576:602	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	3	41	theme	pristine	433:440	arg1	WSA					442:444	pristine WSA	433:444	pristine WSA	433:444	The specific active surface area for pristine WSA and WSA/starch/Fe3O4 magnetic nanocomposites was determined to be 8.1 and 52.6 m2/g, respectively.					
35176297	1	42	theme	shell	225:229	arg1	/starch/Fe3O4					240:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	0	43	theme	shell	48:52	arg1	ash/starch/Fe3O4					54:69	walnut shell ash/starch/Fe3O4	41:69	walnut shell ash/starch/Fe3O4	41:69	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.					
35176297	0	44	dep	Development	0:10	arg1	removal					97:103	removal	97:103	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.	0:138	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.					
35176297	0	44	dep	Development	0:10	arg1	Treatment					106:114	Treatment	106:114	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.	0:138	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.					
35176297	11	45	theme	PSO	1577:1579	arg1	model					1582:1586	the pseudo-second order (PSO) model	1552:1586	the pseudo-second order (PSO) model	1552:1586	The kinetic adsorption data for WSA and WSA/starch/Fe3O4 followed the pseudo-second order (PSO) model.					
35176297	1	46	from	present	272:278	arg1	samples					295:301	groundwater samples	283:301	groundwater samples	283:301	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	6	47	dep	P-	844:845	arg1	The					840:842	The	840:842	The	840:842	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	11	48	theme	order	1570:1574	arg1	model					1582:1586	the pseudo-second order (PSO) model	1552:1586	the pseudo-second order (PSO) model	1552:1586	The kinetic adsorption data for WSA and WSA/starch/Fe3O4 followed the pseudo-second order (PSO) model.					
35176297	1	49	theme	investigation	157:169	arg1	goal					144:147	The goal	140:147	The goal of this investigation	140:169	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	1	50	from	samples	295:301	arg1	present					272:278	present	272:278	present	272:278	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	8	51	theme	highest	1171:1177	arg1	capacity					1196:1203	The highest Cu (II) sorption capacity	1167:1203	The highest Cu (II) sorption capacity of 29.0 and 45.4 mg/g	1167:1225	The highest Cu (II) sorption capacity of 29.0 and 45.4 mg/g was attained for WSA and WSA/starch/Fe3O4, respectively.					
35176297	11	52	theme	kinetic	1490:1496	arg1	data					1509:1512	The kinetic adsorption data	1486:1512	The kinetic adsorption data for WSA and WSA/starch/Fe3O4	1486:1541	The kinetic adsorption data for WSA and WSA/starch/Fe3O4 followed the pseudo-second order (PSO) model.					
35176297	9	53	theme	adsorption	1362:1371	arg1	spontaneous					1384:1394	spontaneous	1384:1394	spontaneous	1384:1394	The free energy of Gibbs had a negative value at 25-45 °C indicating that the adsorption process is spontaneous.					
35176297	9	53	theme	adsorption	1362:1371	arg1	process					1373:1379	the adsorption process	1358:1379	the adsorption process	1358:1379	The free energy of Gibbs had a negative value at 25-45 °C indicating that the adsorption process is spontaneous.					
35176297	0	54	theme	groundwater	119:129	arg1	samples					131:137	groundwater samples	119:137	groundwater samples	119:137	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.					
35176297	5	55	theme	process	780:786	arg1	variables					788:796	process variables	780:796	process variables	780:796	This method showed the success of the model to design process variables and to estimate the appropriate response.					
35176297	6	56	theme	polynomial	888:897	arg1	model					899:903	the quadratic polynomial model	874:903	the quadratic polynomial model	874:903	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	8	57	theme	29.0	1208:1211	arg1	capacity					1196:1203	The highest Cu (II) sorption capacity	1167:1203	The highest Cu (II) sorption capacity of 29.0 and 45.4 mg/g	1167:1225	The highest Cu (II) sorption capacity of 29.0 and 45.4 mg/g was attained for WSA and WSA/starch/Fe3O4, respectively.					
35176297	12	58	theme	adsorbent	1618:1626	arg1	ability					1593:1599	The ability	1589:1599	The ability of the composite adsorbent to remove copper from three groundwater samples	1589:1674	The ability of the composite adsorbent to remove copper from three groundwater samples showed that it could be reused at least 3 times with appropriate efficiency, depending on the water quality.					
35176297	6	59	dep	significance	916:927	arg1	the					912:914	the	912:914	the	912:914	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	8	60	theme	45.4 mg/g	1217:1225	arg1	capacity					1196:1203	The highest Cu (II) sorption capacity	1167:1203	The highest Cu (II) sorption capacity of 29.0 and 45.4 mg/g	1167:1225	The highest Cu (II) sorption capacity of 29.0 and 45.4 mg/g was attained for WSA and WSA/starch/Fe3O4, respectively.					
35176297	5	61	theme	appropriate	818:828	arg1	response					830:837	the appropriate response	814:837	the appropriate response	814:837	This method showed the success of the model to design process variables and to estimate the appropriate response.					
35176297	6	62	theme	model	958:962	arg1	accuracy					933:940	accuracy	933:940	accuracy	933:940	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	6	62	theme	model	958:962	arg1	significance					916:927	significance	916:927	significance	916:927	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	4	63	theme	quantity	657:664	arg1	influence					630:638	the influence	626:638	the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time	626:723	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	0	64	theme	copper	85:90	arg1	ions					92:95	effective copper ions	75:95	effective copper ions	75:95	Development of new magnetic adsorbent of walnut shell ash/starch/Fe3O4 for effective copper ions removal: Treatment of groundwater samples.					
35176297	4	65	theme	initial	667:673	arg1	content					675:681	initial content	667:681	initial content of Cu (II)	667:692	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	7	66	theme	Langmuir	1136:1143	arg1	models					1145:1150	the Freundlich and Langmuir models	1117:1150	models	1145:1150	The Cu adsorption onto WSA and WSA/starch/Fe3O4 obeyed the Freundlich and Langmuir models, respectively.					
35176297	7	67	theme	Freundlich	1121:1130	arg1	models					1145:1150	the Freundlich and Langmuir models	1117:1150	models	1145:1150	The Cu adsorption onto WSA and WSA/starch/Fe3O4 obeyed the Freundlich and Langmuir models, respectively.					
35176297	4	68	theme	time	720:723	arg1	influence					630:638	the influence	626:638	the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time	626:723	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	3	69	theme	active	409:414	arg1	area					424:427	The specific active surface area	396:427	The specific active surface area for pristine WSA and WSA/starch/Fe3O4 magnetic nanocomposites	396:489	The specific active surface area for pristine WSA and WSA/starch/Fe3O4 magnetic nanocomposites was determined to be 8.1 and 52.6 m2/g, respectively.					
35176297	3	69	theme	active	409:414	arg1	8.1					512:514	8.1	512:514	8.1	512:514	The specific active surface area for pristine WSA and WSA/starch/Fe3O4 magnetic nanocomposites was determined to be 8.1 and 52.6 m2/g, respectively.					
35176297	12	70	theme	water	1770:1774	arg1	quality					1776:1782	the water quality	1766:1782	the water quality	1766:1782	The ability of the composite adsorbent to remove copper from three groundwater samples showed that it could be reused at least 3 times with appropriate efficiency, depending on the water quality.					
35176297	4	71	theme	composite	555:563	arg1	design					565:570	A central composite design	545:570	A central composite design for the response surface method	545:602	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	10	72	theme	ΔH	1412:1413	arg1	values					1415:1420	negative ΔH values	1403:1420	negative ΔH values for copper adsorption	1403:1442	Also, negative ΔH values for copper adsorption showed that the processes are exothermic.					
35176297	4	73	theme	temperature	695:705	arg1	influence					630:638	the influence	626:638	the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time	626:723	A central composite design for the response surface method was utilized to study the influence of pH, adsorbent quantity, initial content of Cu (II), temperature, and contact time.					
35176297	1	74	theme	magnetic	192:199	arg1	/starch/Fe3O4					240:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	1	74	theme	magnetic	192:199	arg1	nanocomposite					201:213	a new magnetic nanocomposite	186:213	a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples	186:301	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	3	75	theme	surface	416:422	arg1	area					424:427	The specific active surface area	396:427	The specific active surface area for pristine WSA and WSA/starch/Fe3O4 magnetic nanocomposites	396:489	The specific active surface area for pristine WSA and WSA/starch/Fe3O4 magnetic nanocomposites was determined to be 8.1 and 52.6 m2/g, respectively.					
35176297	3	75	theme	surface	416:422	arg1	8.1					512:514	8.1	512:514	8.1	512:514	The specific active surface area for pristine WSA and WSA/starch/Fe3O4 magnetic nanocomposites was determined to be 8.1 and 52.6 m2/g, respectively.					
35176297	12	76	attach	remove	1631:1636	arg2	ability					1593:1599	The ability	1589:1599	The ability of the composite adsorbent to remove copper from three groundwater samples	1589:1674	The ability of the composite adsorbent to remove copper from three groundwater samples showed that it could be reused at least 3 times with appropriate efficiency, depending on the water quality.					
35176297	12	76	attach	remove	1631:1636	arg1	samples					1668:1674	three groundwater samples	1650:1674	three groundwater samples	1650:1674	The ability of the composite adsorbent to remove copper from three groundwater samples showed that it could be reused at least 3 times with appropriate efficiency, depending on the water quality.					
35176297	2	77	theme	deposition	377:386	arg1	method					388:393	the chemical deposition method	364:393	the chemical deposition method	364:393	The desired nanocomposites were successfully synthesized by the chemical deposition method.					
35176297	7	78	theme	Cu	1066:1067	arg1	adsorption					1069:1078	The Cu adsorption	1062:1078	The Cu adsorption onto WSA and WSA/starch/Fe3O4	1062:1108	The Cu adsorption onto WSA and WSA/starch/Fe3O4 obeyed the Freundlich and Langmuir models, respectively.					
35176297	9	79	theme	free	1288:1291	arg1	energy					1293:1298	The free energy	1284:1298	The free energy of Gibbs	1284:1307	The free energy of Gibbs had a negative value at 25-45 °C indicating that the adsorption process is spontaneous.					
35176297	11	80	theme	adsorption	1498:1507	arg1	data					1509:1512	The kinetic adsorption data	1486:1512	The kinetic adsorption data for WSA and WSA/starch/Fe3O4	1486:1541	The kinetic adsorption data for WSA and WSA/starch/Fe3O4 followed the pseudo-second order (PSO) model.					
35176297	3	81	theme	magnetic	467:474	arg1	nanocomposites					476:489	WSA/starch/Fe3O4 magnetic nanocomposites	450:489	WSA/starch/Fe3O4 magnetic nanocomposites	450:489	The specific active surface area for pristine WSA and WSA/starch/Fe3O4 magnetic nanocomposites was determined to be 8.1 and 52.6 m2/g, respectively.					
35176297	1	82	theme	walnut	218:223	arg1	/starch/Fe3O4					240:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	6	83	theme	0.991	1041:1045	arg1	R2					1014:1015	R2	1014:1015	R2	1014:1015	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	6	83	theme	0.991	1041:1045	arg1	Adj.R2					1021:1026	Adj.R2	1021:1026	Adj.R2	1021:1026	The P- and F-value determined for the quadratic polynomial model showed the significance and accuracy of the proposed model in examining experimental and predicted data with R2 and Adj.R2 of 0.994 and 0.991, respectively.					
35176297	1	84	theme	ash	231:233	arg1	/starch/Fe3O4					240:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	walnut shell ash (WSA)/starch/Fe3O4	218:252	The goal of this investigation was to develop a new magnetic nanocomposite of walnut shell ash (WSA)/starch/Fe3O4 to remove Cu (II) present in groundwater samples.					
35176297	9	85	theme	negative	1315:1322	arg1	value					1324:1328	a negative value	1313:1328	a negative value at 25-45 °C	1313:1340	The free energy of Gibbs had a negative value at 25-45 °C indicating that the adsorption process is spontaneous.					
37192488	0	0	theme	Mitigated	135:143	arg1	Injury					156:161	Mitigated Acute Lung Injury	135:161	Mitigated Acute Lung Injury	135:161	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	4	1	from	officinalis	708:718	arg1	polyphenols					682:692	polyphenols	682:692	polyphenols from Rosemary officinalis	682:718	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	7	2	theme	proinflammatory	1385:1399	arg1	interleukin					1462:1472	interleukin (IL)-1β	1462:1480	interleukin (IL)-1β	1462:1480	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	2	theme	proinflammatory	1385:1399	arg1	chemoattractant					1521:1535	cytokine-induced neutrophil chemoattractant (CINC)-3	1493:1544	cytokine-induced neutrophil chemoattractant (CINC)-3	1493:1544	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	2	theme	proinflammatory	1385:1399	arg1	IL-6					1483:1486	IL-6	1483:1486	IL-6	1483:1486	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	2	theme	proinflammatory	1385:1399	arg1	cytokines					1401:1409	proinflammatory cytokines	1385:1409	proinflammatory cytokines	1385:1409	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	0	3	theme	Murine	166:171	arg1	Models					173:178	Murine Models	166:178	Murine Models	166:178	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	7	4	theme	necrosis	1431:1438	arg1	alpha					1447:1451	chemokine tumor necrosis factor alpha	1415:1451	chemokine tumor necrosis factor alpha (TNF-α)	1415:1459	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	4	theme	necrosis	1431:1438	arg1	TNF-α					1454:1458	TNF-α	1454:1458	TNF-α	1454:1458	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	8	5	dep	%	1757:1757	arg1	reductions					1767:1776	reductions	1767:1776	reductions in the overall lung damage severity	1767:1812	The histopathology data from lung showed statistically significant 37.9% and 37% reductions in the overall lung damage severity and pulmonary edema, respectively, for UP360-treated rats.					
37192488	7	6	theme	chemokine	1415:1423	arg1	alpha					1447:1451	chemokine tumor necrosis factor alpha	1415:1451	chemokine tumor necrosis factor alpha (TNF-α)	1415:1459	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	6	theme	chemokine	1415:1423	arg1	TNF-α					1454:1458	TNF-α	1454:1458	TNF-α	1454:1458	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	8	7	theme	lung	1793:1796	arg1	severity					1805:1812	the overall lung damage severity	1781:1812	the overall lung damage severity	1781:1812	The histopathology data from lung showed statistically significant 37.9% and 37% reductions in the overall lung damage severity and pulmonary edema, respectively, for UP360-treated rats.					
37192488	4	8	from	Poria	666:670	arg1	polysaccharides					625:639	polysaccharides	625:639	polysaccharides from Aloe barbadense and Poria cocos	625:676	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	5	9	theme	500 mg/kg	886:894	arg1	dose					878:881	an oral dose	870:881	an oral dose of 500 mg/kg	870:894	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	7	10	theme	model	1671:1675	arg1	induction					1634:1642	induction	1634:1642	induction of acute lung injury (ALI) model in rats	1634:1683	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	4	11	from	barbadense	651:660	arg1	polysaccharides					625:639	polysaccharides	625:639	polysaccharides from Aloe barbadense and Poria cocos	625:676	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	5	12	from	mice	953:956	arg1	group					981:985	the vehicle control group	961:985	the vehicle control group	961:985	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	10	13	theme	further	2037:2043	arg1	investigation					2045:2057	further investigation	2037:2057	further investigation of the composition for a potential application in human	2037:2113	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	10	13	theme	further	2037:2043	arg1	adjunct					2121:2127	an adjunct	2118:2127	an adjunct supplement in respiratory distress and sepsis	2118:2173	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	8	14	theme	pulmonary	1818:1826	arg1	edema					1828:1832	pulmonary edema	1818:1832	pulmonary edema	1818:1832	The histopathology data from lung showed statistically significant 37.9% and 37% reductions in the overall lung damage severity and pulmonary edema, respectively, for UP360-treated rats.					
37192488	5	15	theme	UP360	850:854	arg1	administration					832:845	Prophylactic oral administration	814:845	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg	814:894	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	5	16	theme	mice	926:929	arg1	rate					918:921	the survival rate	905:921	the survival rate of mice	905:929	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	6	17	theme	%	1188:1188	arg1	rate					1171:1174	a mortality rate	1159:1174	a mortality rate of only 30.8%	1159:1188	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	10	18	theme	composition	2066:2076	arg1	investigation					2045:2057	further investigation	2037:2057	further investigation of the composition for a potential application in human	2037:2113	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	10	18	theme	composition	2066:2076	arg1	adjunct					2121:2127	an adjunct	2118:2127	an adjunct supplement in respiratory distress and sepsis	2118:2173	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	8	19	from	lung	1715:1718	arg1	data					1705:1708	The histopathology data	1686:1708	The histopathology data from lung	1686:1718	The histopathology data from lung showed statistically significant 37.9% and 37% reductions in the overall lung damage severity and pulmonary edema, respectively, for UP360-treated rats.					
37192488	0	20	theme	Acute	145:149	arg1	Injury					156:161	Mitigated Acute Lung Injury	135:161	Mitigated Acute Lung Injury	135:161	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	7	21	theme	lung	1653:1656	arg1	injury					1658:1663	acute lung injury	1647:1663	acute lung injury (ALI) model	1647:1675	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	21	theme	lung	1653:1656	arg1	ALI					1666:1668	ALI	1666:1668	ALI	1666:1668	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	9	22	theme	composition	1888:1898	arg1	UP360					1900:1904	The aloe-based composition UP360	1873:1904	The aloe-based composition UP360	1873:1904	The aloe-based composition UP360 effectively improved the survival rate of septic animals and mitigated the severity of LPS-induced ALI in vivo.					
37192488	10	23	from	distress	2155:2162	arg1	supplement					2129:2138	supplement	2129:2138	supplement	2129:2138	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	8	24	theme	UP360-treated	1853:1865	arg1	rats					1867:1870	UP360-treated rats	1853:1870	UP360-treated rats	1853:1870	The histopathology data from lung showed statistically significant 37.9% and 37% reductions in the overall lung damage severity and pulmonary edema, respectively, for UP360-treated rats.					
37192488	7	25	from	reductions	1371:1380	arg1	chemoattractant					1521:1535	cytokine-induced neutrophil chemoattractant (CINC)-3	1493:1544	cytokine-induced neutrophil chemoattractant (CINC)-3	1493:1544	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	25	from	reductions	1371:1380	arg1	interleukin					1462:1472	interleukin (IL)-1β	1462:1480	interleukin (IL)-1β	1462:1480	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	25	from	reductions	1371:1380	arg1	IL-6					1483:1486	IL-6	1483:1486	IL-6	1483:1486	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	25	from	reductions	1371:1380	arg1	cytokines					1401:1409	proinflammatory cytokines	1385:1409	proinflammatory cytokines	1385:1409	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	25	from	reductions	1371:1380	arg1	alpha					1447:1451	chemokine tumor necrosis factor alpha	1415:1451	chemokine tumor necrosis factor alpha (TNF-α)	1415:1459	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	25	from	reductions	1371:1380	arg1	TNF-α					1454:1458	TNF-α	1454:1458	TNF-α	1454:1458	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	10	26	from	sepsis	2168:2173	arg1	supplement					2129:2138	supplement	2129:2138	supplement	2129:2138	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	6	27	theme	R.	1299:1300	arg1	officinalis					1302:1312	R. officinalis	1299:1312	R. officinalis	1299:1312	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	6	27	theme	R.	1299:1300	arg1	constituents					1257:1268	each individual constituents A. barbadense, P. cocos, and R. officinalis	1241:1312	each individual constituents A. barbadense, P. cocos, and R. officinalis	1241:1312	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	7	28	theme	cytokine-induced	1493:1508	arg1	chemoattractant					1521:1535	cytokine-induced neutrophil chemoattractant (CINC)-3	1493:1544	cytokine-induced neutrophil chemoattractant (CINC)-3	1493:1544	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	28	theme	cytokine-induced	1493:1508	arg1	cytokines					1401:1409	proinflammatory cytokines	1385:1409	proinflammatory cytokines	1385:1409	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	28	theme	cytokine-induced	1493:1508	arg1	CINC					1538:1541	CINC	1538:1541	CINC	1538:1541	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	10	29	theme	supplement	2129:2138	arg1	investigation					2045:2057	further investigation	2037:2057	further investigation of the composition for a potential application in human	2037:2113	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	10	29	theme	supplement	2129:2138	arg1	adjunct					2121:2127	an adjunct	2118:2127	an adjunct supplement in respiratory distress and sepsis	2118:2173	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	4	30	theme	lung	787:790	arg1	injury					792:797	acute inflammatory lung injury	768:797	acute inflammatory lung injury	768:797	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	6	31	theme	mortality	1161:1169	arg1	rate					1171:1174	a mortality rate	1159:1174	a mortality rate of only 30.8%	1159:1188	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	0	32	from	Extracts	39:46	arg1	officinalis					94:104	Rosemary officinalis	85:104	Rosemary officinalis	85:104	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	0	32	from	Extracts	39:46	arg1	UP360					0:4	UP360	0:4	UP360	0:4	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	0	32	from	Extracts	39:46	arg1	Composition					22:32	a Standardized Composition	7:32	a Standardized Composition from Extracts of Aloe barbadense	7:65	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	0	32	from	Extracts	39:46	arg1	cocos					74:78	Poria cocos	68:78	Poria cocos	68:78	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	4	33	theme	murine	799:804	arg1	models					806:811	lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models	724:811	lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models	724:811	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	6	34	theme	P.	1285:1286	arg1	cocos					1288:1292	P. cocos	1285:1292	P. cocos	1285:1292	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	6	34	theme	P.	1285:1286	arg1	constituents					1257:1268	each individual constituents A. barbadense, P. cocos, and R. officinalis	1241:1312	each individual constituents A. barbadense, P. cocos, and R. officinalis	1241:1312	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	5	35	theme	vehicle	965:971	arg1	group					981:985	the vehicle control group	961:985	the vehicle control group	961:985	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	6	36	theme	traditional	1056:1066	arg1	herbs					1068:1072	these traditional herbs	1050:1072	these traditional herbs	1050:1072	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	9	37	theme	septic	1948:1953	arg1	animals					1955:1961	septic animals	1948:1961	septic animals	1948:1961	The aloe-based composition UP360 effectively improved the survival rate of septic animals and mitigated the severity of LPS-induced ALI in vivo.					
37192488	4	38	theme	sepsis	757:762	arg1	models					806:811	lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models	724:811	lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models	724:811	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	1	39	theme	unbalanced	257:266	arg1	response					275:282	a dysregulated and unbalanced immune response	238:282	response	275:282	Sepsis is a life-threatening organ dysfunction caused by a dysregulated and unbalanced immune response to microbial infection.					
37192488	8	40	from	reductions	1767:1776	arg1	severity					1805:1812	the overall lung damage severity	1781:1812	the overall lung damage severity	1781:1812	The histopathology data from lung showed statistically significant 37.9% and 37% reductions in the overall lung damage severity and pulmonary edema, respectively, for UP360-treated rats.					
37192488	10	41	theme	respiratory	2143:2153	arg1	distress					2155:2162	respiratory distress	2143:2162	respiratory distress	2143:2162	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	4	42	theme	acute	768:772	arg1	injury					792:797	acute inflammatory lung injury	768:797	acute inflammatory lung injury	768:797	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	3	43	theme	Natural	415:421	arg1	polysaccharide					442:455	polysaccharide	442:455	polysaccharide	442:455	Natural bioactives such as polysaccharide and polyphenols from botanicals are known for their immune modulation activity.					
37192488	3	43	theme	Natural	415:421	arg1	polyphenols					461:471	polyphenols	461:471	polyphenols	461:471	Natural bioactives such as polysaccharide and polyphenols from botanicals are known for their immune modulation activity.					
37192488	3	43	theme	Natural	415:421	arg1	bioactives					423:432	Natural bioactives	415:432	Natural bioactives such as polysaccharide and polyphenols from botanicals	415:487	Natural bioactives such as polysaccharide and polyphenols from botanicals are known for their immune modulation activity.					
37192488	4	44	theme	standardized	567:578	arg1	UP360					604:608	UP360	604:608	UP360	604:608	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	4	44	theme	standardized	567:578	arg1	composition					591:601	a standardized aloe-based composition	565:601	a standardized aloe-based composition	565:601	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	1	45	theme	microbial	287:295	arg1	infection					297:305	microbial infection	287:305	microbial infection	287:305	Sepsis is a life-threatening organ dysfunction caused by a dysregulated and unbalanced immune response to microbial infection.					
37192488	0	46	theme	Aloe	51:54	arg1	barbadense					56:65	Aloe barbadense	51:65	Aloe barbadense	51:65	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	2	47	theme	infection	341:349	arg1	control					351:357	infection control	341:357	infection control	341:357	Restoring immune homeostasis and infection control are considered the primary strategies to manage sepsis.					
37192488	5	48	theme	oral	827:830	arg1	administration					832:845	Prophylactic oral administration	814:845	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg	814:894	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	9	49	theme	ALI	2005:2007	arg1	severity					1981:1988	the severity	1977:1988	the severity of LPS-induced ALI in vivo	1977:2015	The aloe-based composition UP360 effectively improved the survival rate of septic animals and mitigated the severity of LPS-induced ALI in vivo.					
37192488	1	50	theme	life-threatening	193:208	arg1	dysfunction					216:226	a life-threatening organ dysfunction	191:226	a life-threatening organ dysfunction caused by a dysregulated and unbalanced immune response to microbial infection	191:305	Sepsis is a life-threatening organ dysfunction caused by a dysregulated and unbalanced immune response to microbial infection.					
37192488	1	50	theme	life-threatening	193:208	arg1	Sepsis					181:186	Sepsis	181:186	Sepsis	181:186	Sepsis is a life-threatening organ dysfunction caused by a dysregulated and unbalanced immune response to microbial infection.					
37192488	6	51	theme	composition	1100:1110	arg1	UP360					1112:1116	the standardized composition UP360	1083:1116	the standardized composition UP360	1083:1116	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	5	52	theme	deceased	992:999	arg1	82 h					1001:1004	deceased 82 h	992:1004	deceased 82 h	992:1004	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	3	53	theme	modulation	516:525	arg1	activity					527:534	their immune modulation activity	503:534	their immune modulation activity	503:534	Natural bioactives such as polysaccharide and polyphenols from botanicals are known for their immune modulation activity.					
37192488	7	54	theme	factor	1440:1445	arg1	alpha					1447:1451	chemokine tumor necrosis factor alpha	1415:1451	chemokine tumor necrosis factor alpha (TNF-α)	1415:1459	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	54	theme	factor	1440:1445	arg1	TNF-α					1454:1458	TNF-α	1454:1458	TNF-α	1454:1458	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	4	55	dep	barbadense	651:660	arg1	cocos					672:676	cocos	672:676	cocos	672:676	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	8	56	theme	histopathology	1690:1703	arg1	data					1705:1708	The histopathology data	1686:1708	The histopathology data from lung	1686:1718	The histopathology data from lung showed statistically significant 37.9% and 37% reductions in the overall lung damage severity and pulmonary edema, respectively, for UP360-treated rats.					
37192488	7	57	theme	tumor	1425:1429	arg1	alpha					1447:1451	chemokine tumor necrosis factor alpha	1415:1451	chemokine tumor necrosis factor alpha (TNF-α)	1415:1459	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	57	theme	tumor	1425:1429	arg1	TNF-α					1454:1458	TNF-α	1454:1458	TNF-α	1454:1458	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	58	from	induction	1634:1642	arg1	rats					1680:1683	rats	1680:1683	rats	1680:1683	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	8	59	theme	overall	1785:1791	arg1	severity					1805:1812	the overall lung damage severity	1781:1812	the overall lung damage severity	1781:1812	The histopathology data from lung showed statistically significant 37.9% and 37% reductions in the overall lung damage severity and pulmonary edema, respectively, for UP360-treated rats.					
37192488	5	60	theme	oral	873:876	arg1	dose					878:881	an oral dose	870:881	an oral dose of 500 mg/kg	870:894	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	6	61	dep	demonstrated	1127:1138	arg1	whereas					1191:1197	whereas	1191:1197	whereas	1191:1197	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	8	62	theme	damage	1798:1803	arg1	severity					1805:1812	the overall lung damage severity	1781:1812	the overall lung damage severity	1781:1812	The histopathology data from lung showed statistically significant 37.9% and 37% reductions in the overall lung damage severity and pulmonary edema, respectively, for UP360-treated rats.					
37192488	2	63	theme	Restoring	308:316	arg1	homeostasis					325:335	Restoring immune homeostasis	308:335	Restoring immune homeostasis	308:335	Restoring immune homeostasis and infection control are considered the primary strategies to manage sepsis.					
37192488	7	64	theme	significant	1359:1369	arg1	reductions					1371:1380	Dose-correlated statistically significant reductions	1329:1380	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3	1329:1544	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	65	theme	Dose-correlated	1329:1343	arg1	reductions					1371:1380	Dose-correlated statistically significant reductions	1329:1380	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3	1329:1544	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	10	66	from	application	2094:2104	arg1	human					2109:2113	human	2109:2113	human	2109:2113	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	6	67	theme	A.	1270:1271	arg1	barbadense					1273:1282	A. barbadense	1270:1282	A. barbadense	1270:1282	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	6	67	theme	A.	1270:1271	arg1	constituents					1257:1268	each individual constituents A. barbadense, P. cocos, and R. officinalis	1241:1312	each individual constituents A. barbadense, P. cocos, and R. officinalis	1241:1312	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	4	68	dep	UP360	604:608	arg1	polyphenols					682:692	polyphenols	682:692	polyphenols from Rosemary officinalis	682:718	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	4	68	dep	UP360	604:608	arg1	constitute					611:620	constitute	611:620	constitute	611:620	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	6	69	dep	constituents	1257:1268	arg1	officinalis					1302:1312	R. officinalis	1299:1312	R. officinalis	1299:1312	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	6	69	dep	constituents	1257:1268	arg1	barbadense					1273:1282	A. barbadense	1270:1282	A. barbadense	1270:1282	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	6	69	dep	constituents	1257:1268	arg1	cocos					1288:1292	P. cocos	1285:1292	P. cocos	1285:1292	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	6	69	dep	constituents	1257:1268	arg1	constituents					1257:1268	each individual constituents A. barbadense, P. cocos, and R. officinalis	1241:1312	each individual constituents A. barbadense, P. cocos, and R. officinalis	1241:1312	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	10	70	theme	potential	2084:2092	arg1	application					2094:2104	a potential application	2082:2104	a potential application in human	2082:2113	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	6	71	theme	individual	1246:1255	arg1	officinalis					1302:1312	R. officinalis	1299:1312	R. officinalis	1299:1312	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	6	71	theme	individual	1246:1255	arg1	barbadense					1273:1282	A. barbadense	1270:1282	A. barbadense	1270:1282	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	6	71	theme	individual	1246:1255	arg1	cocos					1288:1292	P. cocos	1285:1292	P. cocos	1285:1292	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	6	71	theme	individual	1246:1255	arg1	constituents					1257:1268	each individual constituents A. barbadense, P. cocos, and R. officinalis	1241:1312	each individual constituents A. barbadense, P. cocos, and R. officinalis	1241:1312	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	0	72	theme	Lung	151:154	arg1	Injury					156:161	Mitigated Acute Lung Injury	135:161	Mitigated Acute Lung Injury	135:161	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	9	73	theme	aloe-based	1877:1886	arg1	UP360					1900:1904	The aloe-based composition UP360	1873:1904	The aloe-based composition UP360	1873:1904	The aloe-based composition UP360 effectively improved the survival rate of septic animals and mitigated the severity of LPS-induced ALI in vivo.					
37192488	10	74	from	supplement	2129:2138	arg1	distress					2155:2162	respiratory distress	2143:2162	respiratory distress	2143:2162	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	10	74	from	supplement	2129:2138	arg1	sepsis					2168:2173	sepsis	2168:2173	sepsis	2168:2173	These data warrant further investigation of the composition for a potential application in human as an adjunct supplement in respiratory distress and sepsis.					
37192488	0	75	theme	barbadense	56:65	arg1	Extracts					39:46	Extracts	39:46	Extracts of Aloe barbadense	39:65	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	7	76	theme	injury	1658:1663	arg1	model					1671:1675	acute lung injury (ALI) model	1647:1675	acute lung injury (ALI) model	1647:1675	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	77	theme	neutrophil	1510:1519	arg1	chemoattractant					1521:1535	cytokine-induced neutrophil chemoattractant (CINC)-3	1493:1544	cytokine-induced neutrophil chemoattractant (CINC)-3	1493:1544	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	77	theme	neutrophil	1510:1519	arg1	cytokines					1401:1409	proinflammatory cytokines	1385:1409	proinflammatory cytokines	1385:1409	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	77	theme	neutrophil	1510:1519	arg1	CINC					1538:1541	CINC	1538:1541	CINC	1538:1541	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	4	78	theme	Rosemary	699:706	arg1	officinalis					708:718	Rosemary officinalis	699:718	Rosemary officinalis	699:718	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	0	79	theme	Poria	68:72	arg1	cocos					74:78	Poria cocos	68:78	Poria cocos	68:78	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	5	80	from	dose	878:881	arg1	administration					832:845	Prophylactic oral administration	814:845	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg	814:894	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	5	81	theme	survival	909:916	arg1	rate					918:921	the survival rate	905:921	the survival rate of mice	905:929	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	7	82	theme	acute	1647:1651	arg1	injury					1658:1663	acute lung injury	1647:1663	acute lung injury (ALI) model	1647:1675	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	7	82	theme	acute	1647:1651	arg1	ALI					1666:1668	ALI	1666:1668	ALI	1666:1668	Dose-correlated statistically significant reductions in proinflammatory cytokines and chemokine tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and cytokine-induced neutrophil chemoattractant (CINC)-3 were observed for UP360 when administered at 250 and 500 mg/kg orally for 7 days before induction of acute lung injury (ALI) model in rats.					
37192488	9	83	theme	survival	1931:1938	arg1	rate					1940:1943	the survival rate	1927:1943	the survival rate of septic animals	1927:1961	The aloe-based composition UP360 effectively improved the survival rate of septic animals and mitigated the severity of LPS-induced ALI in vivo.					
37192488	4	84	theme	inflammatory	774:785	arg1	injury					792:797	acute inflammatory lung injury	768:797	acute inflammatory lung injury	768:797	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	0	85	theme	Rosemary	85:92	arg1	officinalis					94:104	Rosemary officinalis	85:104	Rosemary officinalis	85:104	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	4	86	theme	injury	792:797	arg1	models					806:811	lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models	724:811	lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models	724:811	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	8	87	theme	significant	1741:1751	arg1	%					1757:1757	37.9%	1753:1757	37.9%	1753:1757	The histopathology data from lung showed statistically significant 37.9% and 37% reductions in the overall lung damage severity and pulmonary edema, respectively, for UP360-treated rats.					
37192488	9	88	theme	animals	1955:1961	arg1	rate					1940:1943	the survival rate	1927:1943	the survival rate of septic animals	1927:1961	The aloe-based composition UP360 effectively improved the survival rate of septic animals and mitigated the severity of LPS-induced ALI in vivo.					
37192488	4	89	theme	-induced	748:755	arg1	sepsis					757:762	lipopolysaccharide (LPS)-induced sepsis	724:762	lipopolysaccharide (LPS)-induced sepsis	724:762	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	2	90	theme	primary	378:384	arg1	strategies					386:395	the primary strategies	374:395	the primary strategies	374:395	Restoring immune homeostasis and infection control are considered the primary strategies to manage sepsis.					
37192488	0	91	theme	Standardized	9:20	arg1	Composition					22:32	a Standardized Composition	7:32	a Standardized Composition from Extracts of Aloe barbadense	7:65	UP360, a Standardized Composition from Extracts of Aloe barbadense, Poria cocos, and Rosemary officinalis Protected Against Sepsis and Mitigated Acute Lung Injury in Murine Models.					
37192488	1	92	theme	immune	268:273	arg1	response					275:282	a dysregulated and unbalanced immune response	238:282	response	275:282	Sepsis is a life-threatening organ dysfunction caused by a dysregulated and unbalanced immune response to microbial infection.					
37192488	4	93	theme	aloe-based	580:589	arg1	UP360					604:608	UP360	604:608	UP360	604:608	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	4	93	theme	aloe-based	580:589	arg1	composition					591:601	a standardized aloe-based composition	565:601	a standardized aloe-based composition	565:601	In this study, we evaluated a standardized aloe-based composition, UP360 (constitute of polysaccharides from Aloe barbadense and Poria cocos and polyphenols from Rosemary officinalis) in lipopolysaccharide (LPS)-induced sepsis and acute inflammatory lung injury murine models.					
37192488	5	94	theme	Prophylactic	814:825	arg1	administration					832:845	Prophylactic oral administration	814:845	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg	814:894	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	5	95	theme	LPS	1012:1014	arg1	injection					1016:1024	LPS injection	1012:1024	LPS injection	1012:1024	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	9	96	theme	LPS-induced	1993:2003	arg1	ALI					2005:2007	LPS-induced ALI	1993:2007	LPS-induced ALI in vivo	1993:2015	The aloe-based composition UP360 effectively improved the survival rate of septic animals and mitigated the severity of LPS-induced ALI in vivo.					
37192488	3	97	from	botanicals	478:487	arg1	polysaccharide					442:455	polysaccharide	442:455	polysaccharide	442:455	Natural bioactives such as polysaccharide and polyphenols from botanicals are known for their immune modulation activity.					
37192488	3	97	from	botanicals	478:487	arg1	polyphenols					461:471	polyphenols	461:471	polyphenols	461:471	Natural bioactives such as polysaccharide and polyphenols from botanicals are known for their immune modulation activity.					
37192488	3	98	theme	immune	509:514	arg1	activity					527:534	their immune modulation activity	503:534	their immune modulation activity	503:534	Natural bioactives such as polysaccharide and polyphenols from botanicals are known for their immune modulation activity.					
37192488	5	99	theme	control	973:979	arg1	group					981:985	the vehicle control group	961:985	the vehicle control group	961:985	Prophylactic oral administration of UP360 for 7 days at an oral dose of 500 mg/kg improved the survival rate of mice by 62.5%, whereas all mice in the vehicle control group were deceased 82 h after LPS injection.					
37192488	6	100	dep	merit	1031:1035	arg1	yield					1077:1081	yield	1077:1081	to yield the standardized composition UP360	1074:1116	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
37192488	2	101	theme	immune	318:323	arg1	homeostasis					325:335	Restoring immune homeostasis	308:335	Restoring immune homeostasis	308:335	Restoring immune homeostasis and infection control are considered the primary strategies to manage sepsis.					
37192488	1	102	theme	organ	210:214	arg1	dysfunction					216:226	a life-threatening organ dysfunction	191:226	a life-threatening organ dysfunction caused by a dysregulated and unbalanced immune response to microbial infection	191:305	Sepsis is a life-threatening organ dysfunction caused by a dysregulated and unbalanced immune response to microbial infection.					
37192488	1	102	theme	organ	210:214	arg1	Sepsis					181:186	Sepsis	181:186	Sepsis	181:186	Sepsis is a life-threatening organ dysfunction caused by a dysregulated and unbalanced immune response to microbial infection.					
37192488	6	103	theme	standardized	1087:1098	arg1	UP360					1112:1116	the standardized composition UP360	1083:1116	the standardized composition UP360	1083:1116	The merit of combining these traditional herbs to yield the standardized composition UP360 was also demonstrated in this model with a mortality rate of only 30.8%, whereas 76.9%, 53.9%, and 61.5% were recorded for each individual constituents A. barbadense, P. cocos, and R. officinalis, respectively.					
35499336	8	0	theme	new	1436:1438	arg1	thrombosis					1440:1449	new thrombosis	1436:1449	new thrombosis	1436:1449	No statistical differences were seen in new thrombosis, major bleeding, or any bleeding.					
35499336	9	1	theme	anti-Xa	1579:1585	arg1	monitoring					1587:1596	anti-Xa monitoring	1579:1596	anti-Xa monitoring in relation to clinical outcomes for patients transitioning from an FXai to an UFH infusion	1579:1688	CONCLUSION AND RELEVANCE This represents the first report of a comparison between aPTT versus anti-Xa monitoring in relation to clinical outcomes for patients transitioning from an FXai to an UFH infusion.					
35499336	1	2	from	data	184:187	arg1	strategy					204:211	the optimal strategy	192:211	the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions	192:322	BACKGROUND There are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions.					
35499336	6	3	theme	Secondary	1061:1069	arg1	outcomes					1071:1078	Secondary outcomes	1061:1078	Secondary outcomes	1061:1078	Secondary outcomes were also collected including the amount and duration of UFH administered between cohorts.					
35499336	2	4	theme	UFH	380:382	arg1	infusion					384:391	an UFH infusion	377:391	an UFH infusion	377:391	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	10	5	theme	safe	1782:1785	arg1	alternative					1787:1797	a safe alternative	1780:1797	a safe alternative to aPTT monitoring	1780:1816	A transition guideline primarily utilizing an UFH-calibrated anti-Xa assay appears to be a safe alternative to aPTT monitoring and can aid facilities in the management of patients during these complex transitions.					
35499336	10	5	theme	safe	1782:1785	arg1	guideline					1704:1712	A transition guideline	1691:1712	A transition guideline primarily utilizing an UFH-calibrated anti-Xa assay	1691:1764	A transition guideline primarily utilizing an UFH-calibrated anti-Xa assay appears to be a safe alternative to aPTT monitoring and can aid facilities in the management of patients during these complex transitions.					
35499336	3	6	theme	retrospective	647:659	arg1	analysis					668:675	A retrospective cohort analysis	645:675	A retrospective cohort analysis	645:675	METHODS A retrospective cohort analysis was conducted on adult patients transitioning from an FXai to an UFH infusion at 2 medical centers from June 1, 2018, to November 1, 2020.					
35499336	3	7	from	centers	768:774	arg1	infusion					746:753	an UFH infusion	739:753	an UFH infusion at 2 medical centers from June 1, 2018, to November 1, 2020	739:813	METHODS A retrospective cohort analysis was conducted on adult patients transitioning from an FXai to an UFH infusion at 2 medical centers from June 1, 2018, to November 1, 2020.					
35499336	7	8	theme	aPTT	1277:1280	arg1	group					1282:1286	the aPTT group	1273:1286	the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria	1273:1393	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	2	9	theme	titration	579:587	arg1	scale					589:593	an UFH-calibrated anti-Xa titration scale	553:593	an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline	553:634	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	10	10	theme	anti-Xa	1752:1758	arg1	assay					1760:1764	an UFH-calibrated anti-Xa assay	1734:1764	an UFH-calibrated anti-Xa assay	1734:1764	A transition guideline primarily utilizing an UFH-calibrated anti-Xa assay appears to be a safe alternative to aPTT monitoring and can aid facilities in the management of patients during these complex transitions.					
35499336	10	11	theme	transition	1693:1702	arg1	alternative					1787:1797	a safe alternative	1780:1797	a safe alternative to aPTT monitoring	1780:1816	A transition guideline primarily utilizing an UFH-calibrated anti-Xa assay appears to be a safe alternative to aPTT monitoring and can aid facilities in the management of patients during these complex transitions.					
35499336	10	11	theme	transition	1693:1702	arg1	guideline					1704:1712	A transition guideline	1691:1712	A transition guideline primarily utilizing an UFH-calibrated anti-Xa assay	1691:1764	A transition guideline primarily utilizing an UFH-calibrated anti-Xa assay appears to be a safe alternative to aPTT monitoring and can aid facilities in the management of patients during these complex transitions.					
35499336	2	12	theme	time	508:511	arg1	scale					530:534	an activated partial thromboplastin time (aPTT) titration scale	472:534	an activated partial thromboplastin time (aPTT) titration scale	472:534	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	10	13	theme	complex	1884:1890	arg1	transitions					1892:1902	these complex transitions	1878:1902	these complex transitions	1878:1902	A transition guideline primarily utilizing an UFH-calibrated anti-Xa assay appears to be a safe alternative to aPTT monitoring and can aid facilities in the management of patients during these complex transitions.					
35499336	2	14	theme	anti-Xa	571:577	arg1	scale					589:593	an UFH-calibrated anti-Xa titration scale	553:593	an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline	553:634	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	7	15	theme	endpoint	1223:1230	arg1	%					1239:1239	6.3%	1236:1239	6.3% in the anti-Xa group	1236:1260	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	7	15	theme	endpoint	1223:1230	arg1	rate					1193:1196	The incidence rate	1179:1196	The incidence rate of the primary composite endpoint	1179:1230	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	7	16	theme	noninferiority	1371:1384	arg1	criteria					1386:1393	noninferiority criteria	1371:1393	noninferiority criteria	1371:1393	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	5	17	with	hospitalization	1005:1019	arg1	analysis					1051:1058	a planned noninferiority analysis	1026:1058	a planned noninferiority analysis	1026:1058	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	9	18	theme	UFH	1677:1679	arg1	infusion					1681:1688	an UFH infusion	1674:1688	an UFH infusion	1674:1688	CONCLUSION AND RELEVANCE This represents the first report of a comparison between aPTT versus anti-Xa monitoring in relation to clinical outcomes for patients transitioning from an FXai to an UFH infusion.					
35499336	2	19	theme	thromboplastin	493:506	arg1	aPTT					514:517	aPTT	514:517	aPTT	514:517	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	2	19	theme	thromboplastin	493:506	arg1	time					508:511	partial thromboplastin time	485:511	an activated partial thromboplastin time (aPTT) titration scale	472:534	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	2	20	theme	institutional	612:624	arg1	guideline					626:634	a novel institutional guideline	604:634	a novel institutional guideline	604:634	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	2	21	from	OBJECTIVE	325:333	arg1	patients					338:345	patients	338:345	patients transitioning from an FXai to an UFH infusion	338:391	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	8	22	theme	statistical	1399:1409	arg1	differences					1411:1421	No statistical differences	1396:1421	No statistical differences	1396:1421	No statistical differences were seen in new thrombosis, major bleeding, or any bleeding.					
35499336	10	23	from	facilities	1830:1839	arg1	management					1848:1857	the management	1844:1857	the management of patients	1844:1869	A transition guideline primarily utilizing an UFH-calibrated anti-Xa assay appears to be a safe alternative to aPTT monitoring and can aid facilities in the management of patients during these complex transitions.					
35499336	1	24	theme	inadequate	173:182	arg1	data					184:187	inadequate data	173:187	inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions	173:322	BACKGROUND There are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions.					
35499336	0	25	theme	Treatment	98:106	arg1	Guideline					108:116	a Treatment Guideline	96:116	a Treatment Guideline Utilizing Heparin Anti-Xa Levels	96:149	Comparison of Managing Factor Xa Inhibitor to Unfractionated Heparin Transitions by aPTT Versus a Treatment Guideline Utilizing Heparin Anti-Xa Levels.					
35499336	0	26	theme	Inhibitor	33:41	arg1	Comparison					0:9	Comparison	0:9	Comparison of Managing Factor Xa Inhibitor to Unfractionated Heparin Transitions by aPTT Versus a Treatment Guideline Utilizing Heparin Anti-Xa Levels.	0:150	Comparison of Managing Factor Xa Inhibitor to Unfractionated Heparin Transitions by aPTT Versus a Treatment Guideline Utilizing Heparin Anti-Xa Levels.					
35499336	7	27	from	%	1239:1239	arg1	group					1256:1260	the anti-Xa group	1244:1260	the anti-Xa group	1244:1260	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	7	27	from	%	1239:1239	arg1	group					1282:1286	the aPTT group	1273:1286	the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria	1273:1393	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	6	28	theme	UFH	1137:1139	arg1	duration					1125:1132	duration	1125:1132	duration	1125:1132	Secondary outcomes were also collected including the amount and duration of UFH administered between cohorts.					
35499336	6	28	theme	UFH	1137:1139	arg1	amount					1114:1119	amount	1114:1119	amount	1114:1119	Secondary outcomes were also collected including the amount and duration of UFH administered between cohorts.					
35499336	6	28	theme	UFH	1137:1139	arg1	UFH					1137:1139	UFH	1137:1139	UFH administered between cohorts	1137:1168	Secondary outcomes were also collected including the amount and duration of UFH administered between cohorts.					
35499336	6	29	dep	amount	1114:1119	arg1	the					1110:1112	the	1110:1112	the	1110:1112	Secondary outcomes were also collected including the amount and duration of UFH administered between cohorts.					
35499336	10	30	theme	patients	1862:1869	arg1	management					1848:1857	the management	1844:1857	the management of patients	1844:1869	A transition guideline primarily utilizing an UFH-calibrated anti-Xa assay appears to be a safe alternative to aPTT monitoring and can aid facilities in the management of patients during these complex transitions.					
35499336	4	31	used	utilized	832:839	arg2	institution					820:830	One institution	816:830	One institution	816:830	One institution utilized aPTT while the other institution primarily used UFH-calibrated anti-Xa.					
35499336	7	32	dep	group	1282:1286	arg1	=					1338:1338	=	1338:1338	=	1338:1338	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	7	33	dep	RESULTS	1171:1177	arg1	%					1268:1268	11%	1266:1268	11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria	1266:1393	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	7	33	dep	RESULTS	1171:1177	arg1	rate					1193:1196	The incidence rate	1179:1196	The incidence rate of the primary composite endpoint	1179:1230	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	7	33	dep	RESULTS	1171:1177	arg1	%					1239:1239	6.3%	1236:1239	6.3% in the anti-Xa group	1236:1260	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	3	34	theme	UFH	742:744	arg1	infusion					746:753	an UFH infusion	739:753	an UFH infusion at 2 medical centers from June 1, 2018, to November 1, 2020	739:813	METHODS A retrospective cohort analysis was conducted on adult patients transitioning from an FXai to an UFH infusion at 2 medical centers from June 1, 2018, to November 1, 2020.					
35499336	2	35	dep	safety	418:423	arg1	the					414:416	the	414:416	the	414:416	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	10	36	theme	aPTT	1802:1805	arg1	monitoring					1807:1816	aPTT monitoring	1802:1816	aPTT monitoring	1802:1816	A transition guideline primarily utilizing an UFH-calibrated anti-Xa assay appears to be a safe alternative to aPTT monitoring and can aid facilities in the management of patients during these complex transitions.					
35499336	0	37	theme	Factor	23:28	arg1	Xa					30:31	Managing Factor Xa	14:31	Managing Factor Xa Inhibitor	14:41	Comparison of Managing Factor Xa Inhibitor to Unfractionated Heparin Transitions by aPTT Versus a Treatment Guideline Utilizing Heparin Anti-Xa Levels.					
35499336	0	38	theme	Heparin	128:134	arg1	Levels					144:149	Heparin Anti-Xa Levels	128:149	Heparin Anti-Xa Levels	128:149	Comparison of Managing Factor Xa Inhibitor to Unfractionated Heparin Transitions by aPTT Versus a Treatment Guideline Utilizing Heparin Anti-Xa Levels.					
35499336	5	39	theme	thrombosis	983:992	arg1	death					953:957	death	953:957	death	953:957	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	39	theme	thrombosis	983:992	arg1	endpoint					925:932	The primary endpoint	913:932	The primary endpoint	913:932	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	39	theme	thrombosis	983:992	arg1	composite					940:948	a composite	938:948	a composite of death, major bleeding, or new thrombosis	938:992	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	39	theme	thrombosis	983:992	arg1	bleeding					966:973	bleeding	966:973	bleeding	966:973	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	39	theme	thrombosis	983:992	arg1	thrombosis					983:992	new thrombosis	979:992	new thrombosis	979:992	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	40	theme	primary	917:923	arg1	death					953:957	death	953:957	death	953:957	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	40	theme	primary	917:923	arg1	endpoint					925:932	The primary endpoint	913:932	The primary endpoint	913:932	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	40	theme	primary	917:923	arg1	composite					940:948	a composite	938:948	a composite of death, major bleeding, or new thrombosis	938:992	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	40	theme	primary	917:923	arg1	bleeding					966:973	bleeding	966:973	bleeding	966:973	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	40	theme	primary	917:923	arg1	thrombosis					983:992	new thrombosis	979:992	new thrombosis	979:992	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	1	41	theme	optimal	196:202	arg1	strategy					204:211	the optimal strategy	192:211	the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions	192:322	BACKGROUND There are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions.					
35499336	0	42	theme	Managing	14:21	arg1	Xa					30:31	Managing Factor Xa	14:31	Managing Factor Xa Inhibitor	14:41	Comparison of Managing Factor Xa Inhibitor to Unfractionated Heparin Transitions by aPTT Versus a Treatment Guideline Utilizing Heparin Anti-Xa Levels.					
35499336	2	43	theme	titration	520:528	arg1	scale					530:534	an activated partial thromboplastin time (aPTT) titration scale	472:534	an activated partial thromboplastin time (aPTT) titration scale	472:534	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	9	44	theme	clinical	1613:1620	arg1	outcomes					1622:1629	clinical outcomes	1613:1629	clinical outcomes for patients transitioning from an FXai to an UFH infusion	1613:1688	CONCLUSION AND RELEVANCE This represents the first report of a comparison between aPTT versus anti-Xa monitoring in relation to clinical outcomes for patients transitioning from an FXai to an UFH infusion.					
35499336	1	45	dep	BACKGROUND	152:161	arg1	are					169:171	are	169:171	are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions	169:322	BACKGROUND There are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions.					
35499336	2	46	theme	UFH	452:454	arg1	infusions					456:464	UFH infusions	452:464	UFH infusions using an activated partial thromboplastin time (aPTT) titration scale	452:534	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	1	47	theme	unfractionated	285:298	arg1	UFH					309:311	UFH	309:311	UFH	309:311	BACKGROUND There are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions.					
35499336	1	47	theme	unfractionated	285:298	arg1	heparin					300:306	unfractionated heparin	285:306	unfractionated heparin (UFH) infusions	285:322	BACKGROUND There are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions.					
35499336	10	48	theme	UFH-calibrated	1737:1750	arg1	assay					1760:1764	an UFH-calibrated anti-Xa assay	1734:1764	an UFH-calibrated anti-Xa assay	1734:1764	A transition guideline primarily utilizing an UFH-calibrated anti-Xa assay appears to be a safe alternative to aPTT monitoring and can aid facilities in the management of patients during these complex transitions.					
35499336	5	49	theme	planned	1028:1034	arg1	analysis					1051:1058	a planned noninferiority analysis	1026:1058	a planned noninferiority analysis	1026:1058	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	9	50	theme	first	1530:1534	arg1	report					1536:1541	the first report	1526:1541	the first report of a comparison between aPTT versus anti-Xa monitoring in relation to clinical outcomes for patients transitioning from an FXai to an UFH infusion	1526:1688	CONCLUSION AND RELEVANCE This represents the first report of a comparison between aPTT versus anti-Xa monitoring in relation to clinical outcomes for patients transitioning from an FXai to an UFH infusion.					
35499336	7	51	dep	&lt	1298:1300	arg1	0.001					1303:1307	0.001	1303:1307	0.001	1303:1307	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	0	52	theme	Xa	30:31	arg1	Inhibitor					33:41	Managing Factor Xa Inhibitor	14:41	Managing Factor Xa Inhibitor	14:41	Comparison of Managing Factor Xa Inhibitor to Unfractionated Heparin Transitions by aPTT Versus a Treatment Guideline Utilizing Heparin Anti-Xa Levels.					
35499336	1	53	theme	heparin	300:306	arg1	infusions					314:322	unfractionated heparin (UFH) infusions	285:322	unfractionated heparin (UFH) infusions	285:322	BACKGROUND There are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions.					
35499336	0	54	theme	Anti-Xa	136:142	arg1	Levels					144:149	Heparin Anti-Xa Levels	128:149	Heparin Anti-Xa Levels	128:149	Comparison of Managing Factor Xa Inhibitor to Unfractionated Heparin Transitions by aPTT Versus a Treatment Guideline Utilizing Heparin Anti-Xa Levels.					
35499336	5	55	theme	noninferiority	1036:1049	arg1	analysis					1051:1058	a planned noninferiority analysis	1026:1058	a planned noninferiority analysis	1026:1058	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	2	56	theme	partial	485:491	arg1	aPTT					514:517	aPTT	514:517	aPTT	514:517	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	2	56	theme	partial	485:491	arg1	time					508:511	partial thromboplastin time	485:511	an activated partial thromboplastin time (aPTT) titration scale	472:534	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	0	57	theme	Unfractionated	46:59	arg1	Transitions					69:79	Unfractionated Heparin Transitions	46:79	Unfractionated Heparin Transitions by aPTT Versus a Treatment Guideline Utilizing Heparin Anti-Xa Levels	46:149	Comparison of Managing Factor Xa Inhibitor to Unfractionated Heparin Transitions by aPTT Versus a Treatment Guideline Utilizing Heparin Anti-Xa Levels.					
35499336	3	58	theme	medical	760:766	arg1	centers					768:774	2 medical centers	758:774	2 medical centers from June 1, 2018,	758:793	METHODS A retrospective cohort analysis was conducted on adult patients transitioning from an FXai to an UFH infusion at 2 medical centers from June 1, 2018, to November 1, 2020.					
35499336	2	59	theme	UFH-calibrated	556:569	arg1	scale					589:593	an UFH-calibrated anti-Xa titration scale	553:593	an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline	553:634	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	7	60	theme	primary	1205:1211	arg1	endpoint					1223:1230	the primary composite endpoint	1201:1230	the primary composite endpoint	1201:1230	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	5	61	theme	new	979:981	arg1	thrombosis					983:992	new thrombosis	979:992	new thrombosis	979:992	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	9	62	dep	CONCLUSION	1485:1494	arg1	This					1510:1513	This	1510:1513	This	1510:1513	CONCLUSION AND RELEVANCE This represents the first report of a comparison between aPTT versus anti-Xa monitoring in relation to clinical outcomes for patients transitioning from an FXai to an UFH infusion.					
35499336	4	63	theme	UFH-calibrated	889:902	arg1	anti-Xa					904:910	UFH-calibrated anti-Xa	889:910	UFH-calibrated anti-Xa	889:910	One institution utilized aPTT while the other institution primarily used UFH-calibrated anti-Xa.					
35499336	3	64	from	June	781:784	arg1	centers					768:774	2 medical centers	758:774	2 medical centers from June 1, 2018,	758:793	METHODS A retrospective cohort analysis was conducted on adult patients transitioning from an FXai to an UFH infusion at 2 medical centers from June 1, 2018, to November 1, 2020.					
35499336	2	65	theme	novel	606:610	arg1	guideline					626:634	a novel institutional guideline	604:634	a novel institutional guideline	604:634	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	7	66	theme	incidence	1183:1191	arg1	%					1239:1239	6.3%	1236:1239	6.3% in the anti-Xa group	1236:1260	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	7	66	theme	incidence	1183:1191	arg1	rate					1193:1196	The incidence rate	1179:1196	The incidence rate of the primary composite endpoint	1179:1230	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	7	67	theme	anti-Xa	1248:1254	arg1	group					1256:1260	the anti-Xa group	1244:1260	the anti-Xa group	1244:1260	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	9	68	from	monitoring	1587:1596	arg1	relation					1601:1608	relation	1601:1608	relation to clinical outcomes for patients transitioning from an FXai to an UFH infusion	1601:1688	CONCLUSION AND RELEVANCE This represents the first report of a comparison between aPTT versus anti-Xa monitoring in relation to clinical outcomes for patients transitioning from an FXai to an UFH infusion.					
35499336	9	69	theme	comparison	1548:1557	arg1	report					1536:1541	the first report	1526:1541	the first report of a comparison between aPTT versus anti-Xa monitoring in relation to clinical outcomes for patients transitioning from an FXai to an UFH infusion	1526:1688	CONCLUSION AND RELEVANCE This represents the first report of a comparison between aPTT versus anti-Xa monitoring in relation to clinical outcomes for patients transitioning from an FXai to an UFH infusion.					
35499336	5	70	theme	death	953:957	arg1	death					953:957	death	953:957	death	953:957	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	70	theme	death	953:957	arg1	endpoint					925:932	The primary endpoint	913:932	The primary endpoint	913:932	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	70	theme	death	953:957	arg1	composite					940:948	a composite	938:948	a composite of death, major bleeding, or new thrombosis	938:992	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	70	theme	death	953:957	arg1	bleeding					966:973	bleeding	966:973	bleeding	966:973	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	70	theme	death	953:957	arg1	thrombosis					983:992	new thrombosis	979:992	new thrombosis	979:992	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	7	71	from	%	1268:1268	arg1	group					1256:1260	the anti-Xa group	1244:1260	the anti-Xa group	1244:1260	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	7	71	from	%	1268:1268	arg1	group					1282:1286	the aPTT group	1273:1286	the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria	1273:1393	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	1	72	theme	factor	231:236	arg1	Xa					238:239	factor Xa	231:239	factor Xa inhibitors (FXai; apixaban, rivaroxaban)	231:280	BACKGROUND There are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions.					
35499336	4	73	theme	other	856:860	arg1	institution					862:872	the other institution	852:872	the other institution	852:872	One institution utilized aPTT while the other institution primarily used UFH-calibrated anti-Xa.					
35499336	1	74	dep	FXai	253:256	arg1	rivaroxaban					269:279	rivaroxaban	269:279	rivaroxaban	269:279	BACKGROUND There are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions.					
35499336	1	74	dep	FXai	253:256	arg1	apixaban					259:266	apixaban	259:266	apixaban	259:266	BACKGROUND There are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions.					
35499336	1	75	theme	Xa	238:239	arg1	inhibitors					241:250	factor Xa inhibitors	231:250	factor Xa inhibitors (FXai; apixaban, rivaroxaban)	231:280	BACKGROUND There are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions.					
35499336	1	75	theme	Xa	238:239	arg1	FXai					253:256	FXai	253:256	FXai	253:256	BACKGROUND There are inadequate data on the optimal strategy for transitioning factor Xa inhibitors (FXai; apixaban, rivaroxaban) to unfractionated heparin (UFH) infusions.					
35499336	2	76	theme	activated	475:483	arg1	scale					530:534	an activated partial thromboplastin time (aPTT) titration scale	472:534	an activated partial thromboplastin time (aPTT) titration scale	472:534	OBJECTIVE In patients transitioning from an FXai to an UFH infusion, this study compared the safety and efficacy of monitoring UFH infusions using an activated partial thromboplastin time (aPTT) titration scale versus utilizing an UFH-calibrated anti-Xa titration scale aided by a novel institutional guideline.					
35499336	3	77	theme	cohort	661:666	arg1	analysis					668:675	A retrospective cohort analysis	645:675	A retrospective cohort analysis	645:675	METHODS A retrospective cohort analysis was conducted on adult patients transitioning from an FXai to an UFH infusion at 2 medical centers from June 1, 2018, to November 1, 2020.					
35499336	3	78	dep	METHODS	637:643	arg1	conducted					681:689	conducted	681:689	was conducted on adult patients transitioning from an FXai to an UFH infusion at 2 medical centers from June 1, 2018, to November 1, 2020	677:813	METHODS A retrospective cohort analysis was conducted on adult patients transitioning from an FXai to an UFH infusion at 2 medical centers from June 1, 2018, to November 1, 2020.					
35499336	0	79	theme	Heparin	61:67	arg1	Transitions					69:79	Unfractionated Heparin Transitions	46:79	Unfractionated Heparin Transitions by aPTT Versus a Treatment Guideline Utilizing Heparin Anti-Xa Levels	46:149	Comparison of Managing Factor Xa Inhibitor to Unfractionated Heparin Transitions by aPTT Versus a Treatment Guideline Utilizing Heparin Anti-Xa Levels.					
35499336	3	80	theme	adult	694:698	arg1	patients					700:707	adult patients	694:707	adult patients transitioning from an FXai to an UFH infusion at 2 medical centers from June 1, 2018, to November 1, 2020	694:813	METHODS A retrospective cohort analysis was conducted on adult patients transitioning from an FXai to an UFH infusion at 2 medical centers from June 1, 2018, to November 1, 2020.					
35499336	5	81	theme	bleeding	966:973	arg1	death					953:957	death	953:957	death	953:957	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	81	theme	bleeding	966:973	arg1	endpoint					925:932	The primary endpoint	913:932	The primary endpoint	913:932	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	81	theme	bleeding	966:973	arg1	composite					940:948	a composite	938:948	a composite of death, major bleeding, or new thrombosis	938:992	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	81	theme	bleeding	966:973	arg1	bleeding					966:973	bleeding	966:973	bleeding	966:973	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	5	81	theme	bleeding	966:973	arg1	thrombosis					983:992	new thrombosis	979:992	new thrombosis	979:992	The primary endpoint was a composite of death, major bleeding, or new thrombosis during the hospitalization with a planned noninferiority analysis.					
35499336	7	82	dep	=	1338:1338	arg1	&lt					1298:1300	<i>P</i> &lt	1289:1300	<i>P</i> &lt; 0.001 for noninferiority	1289:1326	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	7	83	theme	composite	1213:1221	arg1	endpoint					1223:1230	the primary composite endpoint	1201:1230	the primary composite endpoint	1201:1230	RESULTS The incidence rate of the primary composite endpoint was 6.3% in the anti-Xa group and 11% in the aPTT group (<i>P</i> &lt; 0.001 for noninferiority, <i>P</i> = 0.138 for superiority) meeting noninferiority criteria.					
35499336	4	84	used	used	884:887	arg2	institution					862:872	the other institution	852:872	the other institution	852:872	One institution utilized aPTT while the other institution primarily used UFH-calibrated anti-Xa.					
35075940	6	0	theme	present	1467:1473	arg1	study					1475:1479	The present study	1463:1479	The present study	1463:1479	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	1	1	theme	insoluble	249:257	arg1	fiber					267:271	citrus insoluble dietary fiber	242:271	citrus insoluble dietary fiber	242:271	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	1	2	mod	modified	387:394	arg3	treatments					435:444	superfine grinding treatments	416:444	superfine grinding treatments	416:444	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	1	2	mod	modified	387:394	arg1	concentrate					371:381	citrus insoluble dietary fiber concentrate	340:381	citrus insoluble dietary fiber concentrate	340:381	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	1	2	mod	modified	387:394	arg3	crush					406:410	coarse crush	399:410	coarse crush	399:410	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	4	3	theme	modified	931:938	arg1	fiber					940:944	modified fiber	931:944	modified fiber	931:944	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	2	4	from	contents	603:610	arg1	cellulose					565:573	cellulose	565:573	cellulose	565:573	The results showed that the contents of hemicellulose and lignin significantly decrease and a significant increase in cellulose and insoluble dietary fiber contents with the reduction in particle size.					
35075940	3	5	theme	dietary	757:763	arg1	powder					783:788	citrus insoluble dietary fiber concentrate powder	740:788	citrus insoluble dietary fiber concentrate powder	740:788	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	5	6	theme	granules	1453:1460	arg1	aggregation					1430:1440	aggregation	1430:1440	aggregation of smaller granules	1430:1460	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	6	7	theme	citrus	1569:1574	arg1	concentrate					1600:1610	citrus insoluble dietary fiber concentrate	1569:1610	citrus insoluble dietary fiber concentrate	1569:1610	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	1	8	theme	fiber	267:271	arg1	properties					228:237	physicochemical, functional, and structural properties	184:237	physicochemical, functional, and structural properties of citrus insoluble dietary fiber	184:271	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	3	9	theme	citrus	740:745	arg1	powder					783:788	citrus insoluble dietary fiber concentrate powder	740:788	citrus insoluble dietary fiber concentrate powder	740:788	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	5	10	theme	particle	1218:1225	arg1	size					1227:1230	particle size	1218:1230	particle size	1218:1230	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	6	11	from	application	1657:1667	arg1	products					1723:1730	food products	1718:1730	food products	1718:1730	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	4	12	theme	fiber	940:944	arg1	crystallinity					892:904	crystallinity	892:904	crystallinity	892:904	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	4	12	theme	fiber	940:944	arg1	stability					918:926	thermal stability	910:926	thermal stability	910:926	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	3	13	theme	concentrate	771:781	arg1	powder					783:788	citrus insoluble dietary fiber concentrate powder	740:788	citrus insoluble dietary fiber concentrate powder	740:788	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	0	14	theme	dietary	108:114	arg1	fiber					116:120	insoluble dietary fiber	98:120	insoluble dietary fiber	98:120	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	5	15	theme	holding	1105:1111	arg1	capacity					1113:1120	water holding capacity	1099:1120	water holding capacity	1099:1120	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	4	16	theme	particle	985:992	arg1	size					994:997	particle size	985:997	particle size	985:997	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	1	17	theme	coarse	399:404	arg1	crush					406:410	coarse crush	399:410	coarse crush	399:410	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	6	18	theme	food	1718:1721	arg1	products					1723:1730	food products	1718:1730	food products	1718:1730	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	0	19	theme	citrus	139:144	arg1	peel					146:149	citrus peel	139:149	citrus peel	139:149	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	2	20	theme	dietary	589:595	arg1	contents					603:610	insoluble dietary fiber contents	579:610	insoluble dietary fiber contents	579:610	The results showed that the contents of hemicellulose and lignin significantly decrease and a significant increase in cellulose and insoluble dietary fiber contents with the reduction in particle size.					
35075940	6	21	theme	concentrate	1703:1713	arg1	application					1657:1667	the application	1653:1667	the application of citrus insoluble dietary fiber concentrate in food products	1653:1730	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	1	22	theme	grinding	426:433	arg1	treatments					435:444	superfine grinding treatments	416:444	superfine grinding treatments	416:444	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	6	23	theme	concentrate	1600:1610	arg1	properties					1555:1564	some properties	1550:1564	some properties	1550:1564	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	6	24	theme	dietary	1689:1695	arg1	concentrate					1703:1713	citrus insoluble dietary fiber concentrate	1672:1713	citrus insoluble dietary fiber concentrate in food products	1672:1730	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	5	25	theme	oil	1152:1154	arg1	capacity					1164:1171	oil holding capacity	1152:1171	oil holding capacity	1152:1171	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	6	26	theme	dietary	1586:1592	arg1	concentrate					1600:1610	citrus insoluble dietary fiber concentrate	1569:1610	citrus insoluble dietary fiber concentrate	1569:1610	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	4	27	theme	partial	1020:1026	arg1	elimination					1028:1038	the partial elimination	1016:1038	the partial elimination of hemicellulose and lignin after the treatments	1016:1087	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	1	28	theme	citrus	340:345	arg1	concentrate					371:381	citrus insoluble dietary fiber concentrate	340:381	citrus insoluble dietary fiber concentrate	340:381	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	6	29	theme	insoluble	1679:1687	arg1	concentrate					1703:1713	citrus insoluble dietary fiber concentrate	1672:1713	citrus insoluble dietary fiber concentrate in food products	1672:1730	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	2	30	theme	significant	541:551	arg1	increase					553:560	a significant increase	539:560	a significant increase in cellulose	539:573	The results showed that the contents of hemicellulose and lignin significantly decrease and a significant increase in cellulose and insoluble dietary fiber contents with the reduction in particle size.					
35075940	0	31	from	Effect	0:5	arg1	composition					27:37	composition	27:37	composition	27:37	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	0	31	from	Effect	0:5	arg1	properties					84:93	physicochemical, functional, and structural properties	40:93	physicochemical, functional, and structural properties of insoluble dietary fiber	40:120	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	5	32	theme	superfine	1276:1284	arg1	treatment					1295:1303	superfine grinding treatment	1276:1303	superfine grinding treatment	1276:1303	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	0	33	theme	structural	73:82	arg1	properties					84:93	physicochemical, functional, and structural properties	40:93	physicochemical, functional, and structural properties of insoluble dietary fiber	40:120	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	0	33	theme	structural	73:82	arg1	composition					27:37	composition	27:37	composition	27:37	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	1	34	theme	dietary	357:363	arg1	concentrate					371:381	citrus insoluble dietary fiber concentrate	340:381	citrus insoluble dietary fiber concentrate	340:381	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	6	35	from	products	1723:1730	arg1	application					1657:1667	the application	1653:1667	the application of citrus insoluble dietary fiber concentrate in food products	1653:1730	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	0	36	theme	particle	10:17	arg1	size					19:22	particle size	10:22	particle size	10:22	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	1	37	theme	citrus	290:295	arg1	peel					297:300	citrus peel	290:300	citrus peel affected by different particle sizes	290:337	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	2	38	from	reduction	621:629	arg1	size					643:646	particle size	634:646	particle size	634:646	The results showed that the contents of hemicellulose and lignin significantly decrease and a significant increase in cellulose and insoluble dietary fiber contents with the reduction in particle size.					
35075940	4	39	theme	concentrate	855:865	arg1	value					815:819	The color value	805:819	The color value of citrus insoluble dietary fiber concentrate	805:865	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	5	40	theme	cellulose	1400:1408	arg1	backbone					1410:1417	the cellulose backbone	1396:1417	the cellulose backbone	1396:1417	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	1	41	theme	different	314:322	arg1	sizes					333:337	different particle sizes	314:337	different particle sizes	314:337	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	4	42	theme	citrus	824:829	arg1	concentrate					855:865	citrus insoluble dietary fiber concentrate	824:865	citrus insoluble dietary fiber concentrate	824:865	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	2	43	from	increase	553:560	arg1	cellulose					565:573	cellulose	565:573	cellulose	565:573	The results showed that the contents of hemicellulose and lignin significantly decrease and a significant increase in cellulose and insoluble dietary fiber contents with the reduction in particle size.					
35075940	3	44	theme	particle	685:692	arg1	size					694:697	the markedly decreased particle size	662:697	the markedly decreased particle size	662:697	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	4	45	theme	dietary	841:847	arg1	concentrate					855:865	citrus insoluble dietary fiber concentrate	824:865	citrus insoluble dietary fiber concentrate	824:865	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	1	46	theme	citrus	242:247	arg1	fiber					267:271	citrus insoluble dietary fiber	242:271	citrus insoluble dietary fiber	242:271	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	4	47	theme	thermal	910:916	arg1	stability					918:926	thermal stability	910:926	thermal stability	910:926	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	3	48	theme	microstructural	713:727	arg1	changes					729:735	obviously microstructural changes	703:735	obviously microstructural changes of citrus insoluble dietary fiber concentrate powder	703:788	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	5	49	theme	smaller	1445:1451	arg1	granules					1453:1460	smaller granules	1445:1460	smaller granules	1445:1460	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	1	50	theme	dietary	259:265	arg1	fiber					267:271	citrus insoluble dietary fiber	242:271	citrus insoluble dietary fiber	242:271	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	3	51	theme	fiber	765:769	arg1	powder					783:788	citrus insoluble dietary fiber concentrate powder	740:788	citrus insoluble dietary fiber concentrate powder	740:788	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	5	52	from	reduction	1205:1213	arg1	size					1227:1230	particle size	1218:1230	particle size	1218:1230	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	6	53	from	concentrate	1703:1713	arg1	products					1723:1730	food products	1718:1730	food products	1718:1730	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	3	54	theme	insoluble	747:755	arg1	powder					783:788	citrus insoluble dietary fiber concentrate powder	740:788	citrus insoluble dietary fiber concentrate powder	740:788	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	2	55	theme	particle	634:641	arg1	size					643:646	particle size	634:646	particle size	634:646	The results showed that the contents of hemicellulose and lignin significantly decrease and a significant increase in cellulose and insoluble dietary fiber contents with the reduction in particle size.					
35075940	0	56	theme	fiber	116:120	arg1	properties					84:93	physicochemical, functional, and structural properties	40:93	physicochemical, functional, and structural properties of insoluble dietary fiber	40:120	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	0	56	theme	fiber	116:120	arg1	composition					27:37	composition	27:37	composition	27:37	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	1	57	theme	physicochemical	184:198	arg1	properties					228:237	physicochemical, functional, and structural properties	184:237	physicochemical, functional, and structural properties of citrus insoluble dietary fiber	184:271	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	3	58	theme	powder	783:788	arg1	changes					729:735	obviously microstructural changes	703:735	obviously microstructural changes of citrus insoluble dietary fiber concentrate powder	703:788	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	3	58	theme	powder	783:788	arg1	size					694:697	the markedly decreased particle size	662:697	the markedly decreased particle size	662:697	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	1	59	theme	functional	201:210	arg1	properties					228:237	physicochemical, functional, and structural properties	184:237	physicochemical, functional, and structural properties of citrus insoluble dietary fiber	184:271	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	3	60	located	observed	795:802	arg1	addition					652:659	addition	652:659	addition	652:659	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	3	60	located	observed	795:802	arg2	size					694:697	the markedly decreased particle size	662:697	the markedly decreased particle size	662:697	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	3	60	located	observed	795:802	arg2	changes					729:735	obviously microstructural changes	703:735	obviously microstructural changes of citrus insoluble dietary fiber concentrate powder	703:788	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	5	61	theme	water	1123:1127	arg1	capacity					1138:1145	water swelling capacity	1123:1145	water swelling capacity	1123:1145	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	2	62	theme	fiber	597:601	arg1	contents					603:610	insoluble dietary fiber contents	579:610	insoluble dietary fiber contents	579:610	The results showed that the contents of hemicellulose and lignin significantly decrease and a significant increase in cellulose and insoluble dietary fiber contents with the reduction in particle size.					
35075940	5	63	theme	grinding	1286:1293	arg1	treatment					1295:1303	superfine grinding treatment	1276:1303	superfine grinding treatment	1276:1303	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	1	64	theme	superfine	416:424	arg1	treatments					435:444	superfine grinding treatments	416:444	superfine grinding treatments	416:444	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	2	65	theme	insoluble	579:587	arg1	contents					603:610	insoluble dietary fiber contents	579:610	insoluble dietary fiber contents	579:610	The results showed that the contents of hemicellulose and lignin significantly decrease and a significant increase in cellulose and insoluble dietary fiber contents with the reduction in particle size.					
35075940	6	66	theme	fiber	1697:1701	arg1	concentrate					1703:1713	citrus insoluble dietary fiber concentrate	1672:1713	citrus insoluble dietary fiber concentrate in food products	1672:1730	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	0	67	theme	functional	57:66	arg1	properties					84:93	physicochemical, functional, and structural properties	40:93	physicochemical, functional, and structural properties of insoluble dietary fiber	40:120	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	0	67	theme	functional	57:66	arg1	composition					27:37	composition	27:37	composition	27:37	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	5	68	theme	water	1099:1103	arg1	capacity					1113:1120	water holding capacity	1099:1120	water holding capacity	1099:1120	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	6	69	theme	fiber	1594:1598	arg1	concentrate					1600:1610	citrus insoluble dietary fiber concentrate	1569:1610	citrus insoluble dietary fiber concentrate	1569:1610	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	2	70	theme	lignin	505:510	arg1	contents					475:482	the contents	471:482	the contents of hemicellulose and lignin	471:510	The results showed that the contents of hemicellulose and lignin significantly decrease and a significant increase in cellulose and insoluble dietary fiber contents with the reduction in particle size.					
35075940	5	71	theme	holding	1156:1162	arg1	capacity					1164:1171	oil holding capacity	1152:1171	oil holding capacity	1152:1171	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	6	72	theme	insoluble	1576:1584	arg1	concentrate					1600:1610	citrus insoluble dietary fiber concentrate	1569:1610	citrus insoluble dietary fiber concentrate	1569:1610	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	5	73	theme	swelling	1129:1136	arg1	capacity					1138:1145	water swelling capacity	1123:1145	water swelling capacity	1123:1145	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	2	74	theme	hemicellulose	487:499	arg1	contents					475:482	the contents	471:482	the contents of hemicellulose and lignin	471:510	The results showed that the contents of hemicellulose and lignin significantly decrease and a significant increase in cellulose and insoluble dietary fiber contents with the reduction in particle size.					
35075940	1	75	theme	insoluble	347:355	arg1	concentrate					371:381	citrus insoluble dietary fiber concentrate	340:381	citrus insoluble dietary fiber concentrate	340:381	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	6	76	theme	citrus	1672:1677	arg1	concentrate					1703:1713	citrus insoluble dietary fiber concentrate	1672:1713	citrus insoluble dietary fiber concentrate in food products	1672:1730	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	0	77	theme	insoluble	98:106	arg1	fiber					116:120	insoluble dietary fiber	98:120	insoluble dietary fiber	98:120	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	1	78	theme	fiber	365:369	arg1	concentrate					371:381	citrus insoluble dietary fiber concentrate	340:381	citrus insoluble dietary fiber concentrate	340:381	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	4	79	theme	color	809:813	arg1	value					815:819	The color value	805:819	The color value of citrus insoluble dietary fiber concentrate	805:865	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	5	80	theme	soluble	1358:1364	arg1	components					1366:1375	some soluble components	1353:1375	some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules	1353:1460	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	4	81	theme	lignin	1061:1066	arg1	elimination					1028:1038	the partial elimination	1016:1038	the partial elimination of hemicellulose and lignin after the treatments	1016:1087	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	5	82	theme	structure	1319:1327	arg1	integrity					1329:1337	the structure integrity	1315:1337	the structure integrity	1315:1337	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	0	83	theme	size	19:22	arg1	Effect					0:5	Effect	0:5	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber	0:120	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	6	84	theme	new	1632:1634	arg1	perspectives					1636:1647	new perspectives	1632:1647	new perspectives for the application of citrus insoluble dietary fiber concentrate in food products	1632:1730	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	5	85	from	backbone	1410:1417	arg1	break					1380:1384	break	1380:1384	break away from the cellulose backbone	1380:1417	However, water holding capacity, water swelling capacity, and oil holding capacity were found to be lower with the reduction in particle size, which might be attributed to the fact that superfine grinding treatment destroyed the structure integrity, thus causing some soluble components to break away from the cellulose backbone, or due to aggregation of smaller granules.					
35075940	4	86	theme	fiber	849:853	arg1	concentrate					855:865	citrus insoluble dietary fiber concentrate	824:865	citrus insoluble dietary fiber concentrate	824:865	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	0	87	theme	physicochemical	40:54	arg1	properties					84:93	physicochemical, functional, and structural properties	40:93	physicochemical, functional, and structural properties of insoluble dietary fiber	40:120	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	0	87	theme	physicochemical	40:54	arg1	composition					27:37	composition	27:37	composition	27:37	Effect of particle size on composition, physicochemical, functional, and structural properties of insoluble dietary fiber concentrate from citrus peel.					
35075940	4	88	theme	hemicellulose	1043:1055	arg1	elimination					1028:1038	the partial elimination	1016:1038	the partial elimination of hemicellulose and lignin after the treatments	1016:1087	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	4	89	from	decrease	973:980	arg1	size					994:997	particle size	985:997	particle size	985:997	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
35075940	6	90	theme	particle	1511:1518	arg1	size					1520:1523	the particle size	1507:1523	the particle size	1507:1523	The present study suggested that decreasing the particle size could effectively change some properties of citrus insoluble dietary fiber concentrate, which will provide new perspectives for the application of citrus insoluble dietary fiber concentrate in food products.					
35075940	1	91	theme	particle	324:331	arg1	sizes					333:337	different particle sizes	314:337	different particle sizes	314:337	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	3	92	theme	decreased	675:683	arg1	size					694:697	the markedly decreased particle size	662:697	the markedly decreased particle size	662:697	In addition, the markedly decreased particle size and obviously microstructural changes of citrus insoluble dietary fiber concentrate powder were observed.					
35075940	1	93	theme	structural	217:226	arg1	properties					228:237	physicochemical, functional, and structural properties	184:237	physicochemical, functional, and structural properties of citrus insoluble dietary fiber	184:271	To investigate the composition, physicochemical, functional, and structural properties of citrus insoluble dietary fiber concentrate from citrus peel affected by different particle sizes, citrus insoluble dietary fiber concentrate was modified by coarse crush and superfine grinding treatments.					
35075940	4	94	theme	insoluble	831:839	arg1	concentrate					855:865	citrus insoluble dietary fiber concentrate	824:865	citrus insoluble dietary fiber concentrate	824:865	The color value of citrus insoluble dietary fiber concentrate was observably improved, crystallinity and thermal stability of modified fiber slightly increase with the decrease in particle size, which is due to the partial elimination of hemicellulose and lignin after the treatments.					
36939098	6	0	theme	peptide	997:1003	arg1	pKa					974:976	The pKa	970:976	The pKa of the temporin-Ra peptide	970:1003	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	5	1	with	cross-linking	718:730	arg1	acid					744:747	citric acid	737:747	citric acid	737:747	Green cross-linking with citric acid prevented the destruction of the nanofiber even after prolonged immersion in biological solutions.					
36939098	14	2	theme	healing	2179:2185	arg1	phase					2164:2168	the inflammatory phase	2147:2168	the inflammatory phase of wound healing	2147:2185	Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.					
36939098	13	3	theme	good	2032:2035	arg1	alternative					2037:2047	a good alternative	2030:2047	a good alternative to existing wound dressings	2030:2075	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	13	3	theme	good	2032:2035	arg1	mats					2018:2021	these active nanofiber mats	1995:2021	these active nanofiber mats	1995:2021	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	12	4	theme	control	1854:1860	arg1	group					1862:1866	the control group	1850:1866	the control group (17%)	1850:1872	On day 6, the peptide-loaded nanofiber revealed 60% wound closure compared to the control group (17%).					
36939098	12	4	theme	control	1854:1860	arg1	%					1871:1871	17%	1869:1871	17%	1869:1871	On day 6, the peptide-loaded nanofiber revealed 60% wound closure compared to the control group (17%).					
36939098	6	5	from	pH.	966:968	arg1	charge					935:940	a negative surface charge	916:940	a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions	916:1109	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	6	5	from	pH.	966:968	arg1	∼-30					943:946	∼-30	943:946	∼-30	943:946	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	5	6	theme	Green	712:716	arg1	cross-linking					718:730	Green cross-linking	712:730	Green cross-linking with citric acid	712:747	Green cross-linking with citric acid prevented the destruction of the nanofiber even after prolonged immersion in biological solutions.					
36939098	4	7	theme	dressing	702:709	arg1	requirements					670:681	the requirements	666:681	the requirements of the ideal wound dressing	666:709	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	14	8	theme	factor-α	2246:2253	arg1	cytokine					2255:2262	the tumor necrosis factor-α cytokine	2227:2262	the tumor necrosis factor-α cytokine response	2227:2271	Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.					
36939098	5	9	theme	nanofiber	782:790	arg1	destruction					763:773	the destruction	759:773	the destruction of the nanofiber	759:790	Green cross-linking with citric acid prevented the destruction of the nanofiber even after prolonged immersion in biological solutions.					
36939098	4	10	theme	composite	539:547	arg1	nanofiber					549:557	the synthesized composite nanofiber	523:557	the synthesized composite nanofiber	523:557	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	14	11	theme	tumor	2231:2235	arg1	cytokine					2255:2262	the tumor necrosis factor-α cytokine	2227:2262	the tumor necrosis factor-α cytokine response	2227:2271	Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.					
36939098	7	12	theme	peptide	1123:1129	arg1	molecules					1131:1139	peptide molecules	1123:1139	peptide molecules	1123:1139	Therefore, peptide molecules immobilized on the synthesized scaffold based on surface adsorption.					
36939098	3	13	theme	alcohol	383:389	arg1	nanofibers					399:408	hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers	348:408	hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility	348:446	In this regard, hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility were produced by optimizing the solution preparation process.					
36939098	1	14	theme	excellent	149:157	arg1	potential					159:167	excellent potential	149:167	excellent potential	149:167	Bioresponsive nanodrug delivery systems have excellent potential in tissue engineering applications.					
36939098	12	15	theme	60	1820:1821	arg1	%					1822:1822	%	1822:1822	%	1822:1822	On day 6, the peptide-loaded nanofiber revealed 60% wound closure compared to the control group (17%).					
36939098	3	16	theme	hyaluronic	348:357	arg1	nanofibers					399:408	hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers	348:408	hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility	348:446	In this regard, hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility were produced by optimizing the solution preparation process.					
36939098	3	17	with	nanofibers	399:408	arg1	reproducibility					432:446	reproducibility	432:446	reproducibility	432:446	In this regard, hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility were produced by optimizing the solution preparation process.					
36939098	3	17	with	nanofibers	399:408	arg1	quality					420:426	high quality	415:426	high quality	415:426	In this regard, hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility were produced by optimizing the solution preparation process.					
36939098	12	18	theme	wound	1824:1828	arg1	closure					1830:1836	60% wound closure	1820:1836	60% wound closure	1820:1836	On day 6, the peptide-loaded nanofiber revealed 60% wound closure compared to the control group (17%).					
36939098	3	19	theme	high	415:418	arg1	quality					420:426	high quality	415:426	high quality	415:426	In this regard, hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility were produced by optimizing the solution preparation process.					
36939098	14	20	theme	Gene	2078:2081	arg1	studies					2094:2100	Gene expression studies	2078:2100	Gene expression studies	2078:2100	Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.					
36939098	13	21	theme	nanofiber	2008:2016	arg1	alternative					2037:2047	a good alternative	2030:2047	a good alternative to existing wound dressings	2030:2075	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	13	21	theme	nanofiber	2008:2016	arg1	mats					2018:2021	these active nanofiber mats	1995:2021	these active nanofiber mats	1995:2021	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	2	22	theme	drug	309:312	arg1	systems					323:329	pH-sensitive drug delivery systems	296:329	pH-sensitive drug delivery systems	296:329	Poly-anionic and poly-cationic biopolymers have provided a superior platform for designing pH-sensitive drug delivery systems.					
36939098	8	23	from	scaffold	1336:1343	arg1	release					1309:1315	peptide release	1301:1315	peptide release from the nanofiber scaffold	1301:1343	In vivo evaluation has proven that the wound bed has an alkaline environment, facilitating peptide release from the nanofiber scaffold.					
36939098	1	24	theme	engineering	179:189	arg1	applications					191:202	tissue engineering applications	172:202	tissue engineering applications	172:202	Bioresponsive nanodrug delivery systems have excellent potential in tissue engineering applications.					
36939098	6	25	theme	net	1063:1065	arg1	result					1027:1032	a result	1025:1032	a result	1025:1032	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	6	25	theme	net	1063:1065	arg1	charge					1076:1081	a net positive charge	1061:1081	a net positive charge	1061:1081	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	6	26	contain	has	912:914	arg1	nanofiber					902:910	the synthesized nanofiber	886:910	the synthesized nanofiber	886:910	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	6	26	contain	has	912:914	arg2	∼-30					943:946	∼-30	943:946	∼-30	943:946	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	6	26	contain	has	912:914	arg2	charge					935:940	a negative surface charge	916:940	a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions	916:1109	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	4	27	theme	200	588:590	arg1	%					591:591	%	591:591	%	591:591	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	10	28	theme	cell	1546:1549	arg1	growth					1551:1556	fibroblast cell growth	1535:1556	fibroblast cell growth	1535:1556	In vitro investigation showed better adhesion, proliferation, migration, and fibroblast cell growth on peptide-loaded nanofiber samples than other groups.					
36939098	13	29	theme	repair	1973:1978	arg1	ability					1980:1986	excellent tissue repair ability	1956:1986	excellent tissue repair ability	1956:1986	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	4	30	theme	swelling	593:600	arg1	ratio					602:606	200% swelling ratio	588:606	200% swelling ratio	588:606	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	8	31	dep	proven	1233:1238	arg1	has					1259:1261	has	1259:1261	has	1259:1261	In vivo evaluation has proven that the wound bed has an alkaline environment, facilitating peptide release from the nanofiber scaffold.					
36939098	11	32	theme	In	1613:1614	arg1	studies					1621:1627	In vivo studies	1613:1627	In vivo studies on full-thickness wounds in the mouse model	1613:1671	In vivo studies on full-thickness wounds in the mouse model indicated that the designed nanofiber was gradually absorbed without causing dryness or infection.					
36939098	6	33	theme	peptide	1038:1044	arg1	molecules					1046:1054	the peptide molecules	1034:1054	the peptide molecules	1034:1054	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	4	34	theme	66.82	565:569	arg1	toughness					577:585	66.82 kN/mm toughness	565:585	66.82 kN/mm toughness	565:585	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	10	35	theme	nanofiber	1576:1584	arg1	samples					1586:1592	peptide-loaded nanofiber samples	1561:1592	peptide-loaded nanofiber samples	1561:1592	In vitro investigation showed better adhesion, proliferation, migration, and fibroblast cell growth on peptide-loaded nanofiber samples than other groups.					
36939098	5	36	theme	biological	826:835	arg1	solutions					837:845	biological solutions	826:845	biological solutions	826:845	Green cross-linking with citric acid prevented the destruction of the nanofiber even after prolonged immersion in biological solutions.					
36939098	11	37	from	studies	1621:1627	arg1	wounds					1647:1652	full-thickness wounds	1632:1652	full-thickness wounds in the mouse model	1632:1671	In vivo studies on full-thickness wounds in the mouse model indicated that the designed nanofiber was gradually absorbed without causing dryness or infection.					
36939098	8	38	dep	In	1210:1211	arg1	vivo					1213:1216	vivo	1213:1216	vivo	1213:1216	In vivo evaluation has proven that the wound bed has an alkaline environment, facilitating peptide release from the nanofiber scaffold.					
36939098	11	39	dep	In	1613:1614	arg1	vivo					1616:1619	vivo	1616:1619	vivo	1616:1619	In vivo studies on full-thickness wounds in the mouse model indicated that the designed nanofiber was gradually absorbed without causing dryness or infection.					
36939098	13	40	theme	excellent	1956:1964	arg1	repair					1973:1978	excellent tissue repair	1956:1978	excellent tissue repair ability	1956:1986	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	4	41	with	nanofiber	549:557	arg1	toughness					577:585	66.82 kN/mm toughness	565:585	66.82 kN/mm toughness	565:585	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	4	41	with	nanofiber	549:557	arg1	porosity					617:624	60% porosity	613:624	60% porosity	613:624	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	4	41	with	nanofiber	549:557	arg1	ratio					602:606	200% swelling ratio	588:606	200% swelling ratio	588:606	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	0	42	theme	Cross-Linked	0:11	arg1	Nanofibers					38:47	Cross-Linked Electrospun pH-Sensitive Nanofibers	0:47	Cross-Linked Electrospun pH-Sensitive Nanofibers	0:47	Cross-Linked Electrospun pH-Sensitive Nanofibers Adsorbed with Temporin-Ra for Promoting Wound Healing.					
36939098	9	43	theme	matrix	1419:1424	arg1	architecture					1385:1396	the architecture	1381:1396	the architecture of the extracellular matrix for accelerating wound healing	1381:1455	Electrospun nanofibers can imitate the architecture of the extracellular matrix for accelerating wound healing.					
36939098	1	44	theme	nanodrug	118:125	arg1	systems					136:142	Bioresponsive nanodrug delivery systems	104:142	Bioresponsive nanodrug delivery systems	104:142	Bioresponsive nanodrug delivery systems have excellent potential in tissue engineering applications.					
36939098	11	45	theme	full-thickness	1632:1645	arg1	wounds					1647:1652	full-thickness wounds	1632:1652	full-thickness wounds in the mouse model	1632:1671	In vivo studies on full-thickness wounds in the mouse model indicated that the designed nanofiber was gradually absorbed without causing dryness or infection.					
36939098	6	46	contain	have	1056:1059	arg2	charge					1076:1081	a net positive charge	1061:1081	a net positive charge	1061:1081	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	6	46	contain	have	1056:1059	arg2	result					1027:1032	a result	1025:1032	a result	1025:1032	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	6	46	contain	have	1056:1059	arg1	molecules					1046:1054	the peptide molecules	1034:1054	the peptide molecules	1034:1054	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	0	47	theme	pH-Sensitive	25:36	arg1	Nanofibers					38:47	Cross-Linked Electrospun pH-Sensitive Nanofibers	0:47	Cross-Linked Electrospun pH-Sensitive Nanofibers	0:47	Cross-Linked Electrospun pH-Sensitive Nanofibers Adsorbed with Temporin-Ra for Promoting Wound Healing.					
36939098	6	48	dep	charge	935:940	arg1	have					1056:1059	have	1056:1059	have a net positive charge in physiological conditions	1056:1109	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	6	48	dep	charge	935:940	arg1	10					1014:1015	10	1014:1015	10	1014:1015	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	14	49	theme	inflammatory	2151:2162	arg1	phase					2164:2168	the inflammatory phase	2147:2168	the inflammatory phase of wound healing	2147:2185	Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.					
36939098	2	50	theme	poly-cationic	222:234	arg1	biopolymers					236:246	Poly-anionic and poly-cationic biopolymers	205:246	Poly-anionic and poly-cationic biopolymers	205:246	Poly-anionic and poly-cationic biopolymers have provided a superior platform for designing pH-sensitive drug delivery systems.					
36939098	13	51	dep	demonstrated	1943:1954	arg1	alternative					2037:2047	a good alternative	2030:2047	a good alternative to existing wound dressings	2030:2075	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	13	51	dep	demonstrated	1943:1954	arg1	mats					2018:2021	these active nanofiber mats	1995:2021	these active nanofiber mats	1995:2021	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	6	52	theme	physiological	1086:1098	arg1	conditions					1100:1109	physiological conditions	1086:1109	physiological conditions	1086:1109	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	3	53	theme	preparation	489:499	arg1	process					501:507	the solution preparation process	476:507	the solution preparation process	476:507	In this regard, hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility were produced by optimizing the solution preparation process.					
36939098	2	54	theme	Poly-anionic	205:216	arg1	biopolymers					236:246	Poly-anionic and poly-cationic biopolymers	205:246	Poly-anionic and poly-cationic biopolymers	205:246	Poly-anionic and poly-cationic biopolymers have provided a superior platform for designing pH-sensitive drug delivery systems.					
36939098	6	55	theme	ζ	848:848	arg1	studies					860:866	ζ potential studies	848:866	ζ potential studies	848:866	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	13	56	theme	existing	2052:2059	arg1	dressings					2067:2075	existing wound dressings	2052:2075	existing wound dressings	2052:2075	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	4	57	theme	60	613:614	arg1	%					615:615	%	615:615	%	615:615	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	14	58	theme	antimicrobial	2116:2128	arg1	peptide					2130:2136	the antimicrobial peptide	2112:2136	the antimicrobial peptide	2112:2136	Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.					
36939098	6	59	theme	surface	927:933	arg1	charge					935:940	a negative surface charge	916:940	a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions	916:1109	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	6	59	theme	surface	927:933	arg1	∼-30					943:946	∼-30	943:946	∼-30	943:946	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	11	60	theme	designed	1692:1699	arg1	nanofiber					1701:1709	the designed nanofiber	1688:1709	the designed nanofiber	1688:1709	In vivo studies on full-thickness wounds in the mouse model indicated that the designed nanofiber was gradually absorbed without causing dryness or infection.					
36939098	10	61	theme	In	1458:1459	arg1	investigation					1467:1479	In vitro investigation	1458:1479	In vitro investigation	1458:1479	In vitro investigation showed better adhesion, proliferation, migration, and fibroblast cell growth on peptide-loaded nanofiber samples than other groups.					
36939098	7	62	theme	surface	1190:1196	arg1	adsorption					1198:1207	surface adsorption	1190:1207	surface adsorption	1190:1207	Therefore, peptide molecules immobilized on the synthesized scaffold based on surface adsorption.					
36939098	6	63	theme	temporin-Ra	985:995	arg1	peptide					997:1003	the temporin-Ra peptide	981:1003	the temporin-Ra peptide	981:1003	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	1	64	contain	have	144:147	arg2	potential					159:167	excellent potential	149:167	excellent potential	149:167	Bioresponsive nanodrug delivery systems have excellent potential in tissue engineering applications.					
36939098	1	64	contain	have	144:147	arg1	systems					136:142	Bioresponsive nanodrug delivery systems	104:142	Bioresponsive nanodrug delivery systems	104:142	Bioresponsive nanodrug delivery systems have excellent potential in tissue engineering applications.					
36939098	5	65	theme	citric	737:742	arg1	acid					744:747	citric acid	737:747	citric acid	737:747	Green cross-linking with citric acid prevented the destruction of the nanofiber even after prolonged immersion in biological solutions.					
36939098	10	66	dep	better	1488:1493	arg1	adhesion					1495:1502	adhesion	1495:1502	adhesion	1495:1502	In vitro investigation showed better adhesion, proliferation, migration, and fibroblast cell growth on peptide-loaded nanofiber samples than other groups.					
36939098	7	67	theme	synthesized	1160:1170	arg1	scaffold					1172:1179	the synthesized scaffold	1156:1179	the synthesized scaffold based on surface adsorption	1156:1207	Therefore, peptide molecules immobilized on the synthesized scaffold based on surface adsorption.					
36939098	6	68	theme	synthesized	890:900	arg1	nanofiber					902:910	the synthesized nanofiber	886:910	the synthesized nanofiber	886:910	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	8	69	theme	peptide	1301:1307	arg1	release					1309:1315	peptide release	1301:1315	peptide release from the nanofiber scaffold	1301:1343	In vivo evaluation has proven that the wound bed has an alkaline environment, facilitating peptide release from the nanofiber scaffold.					
36939098	10	70	dep	In	1458:1459	arg1	vitro					1461:1465	vitro	1461:1465	vitro	1461:1465	In vitro investigation showed better adhesion, proliferation, migration, and fibroblast cell growth on peptide-loaded nanofiber samples than other groups.					
36939098	8	71	theme	wound	1249:1253	arg1	bed					1255:1257	the wound bed	1245:1257	the wound bed	1245:1257	In vivo evaluation has proven that the wound bed has an alkaline environment, facilitating peptide release from the nanofiber scaffold.					
36939098	14	72	theme	cytokine	2255:2262	arg1	response					2264:2271	the tumor necrosis factor-α cytokine response	2227:2271	the tumor necrosis factor-α cytokine response	2227:2271	Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.					
36939098	11	73	theme	mouse	1661:1665	arg1	model					1667:1671	the mouse model	1657:1671	the mouse model	1657:1671	In vivo studies on full-thickness wounds in the mouse model indicated that the designed nanofiber was gradually absorbed without causing dryness or infection.					
36939098	8	74	theme	nanofiber	1326:1334	arg1	scaffold					1336:1343	the nanofiber scaffold	1322:1343	the nanofiber scaffold	1322:1343	In vivo evaluation has proven that the wound bed has an alkaline environment, facilitating peptide release from the nanofiber scaffold.					
36939098	14	75	theme	necrosis	2237:2244	arg1	cytokine					2255:2262	the tumor necrosis factor-α cytokine	2227:2262	the tumor necrosis factor-α cytokine response	2227:2271	Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.					
36939098	2	76	theme	pH-sensitive	296:307	arg1	systems					323:329	pH-sensitive drug delivery systems	296:329	pH-sensitive drug delivery systems	296:329	Poly-anionic and poly-cationic biopolymers have provided a superior platform for designing pH-sensitive drug delivery systems.					
36939098	8	77	theme	alkaline	1266:1273	arg1	environment					1275:1285	an alkaline environment	1263:1285	an alkaline environment	1263:1285	In vivo evaluation has proven that the wound bed has an alkaline environment, facilitating peptide release from the nanofiber scaffold.					
36939098	5	78	from	immersion	813:821	arg1	solutions					837:845	biological solutions	826:845	biological solutions	826:845	Green cross-linking with citric acid prevented the destruction of the nanofiber even after prolonged immersion in biological solutions.					
36939098	3	79	theme	complex	391:397	arg1	nanofibers					399:408	hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers	348:408	hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility	348:446	In this regard, hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility were produced by optimizing the solution preparation process.					
36939098	14	80	theme	time	2200:2203	arg1	frame					2205:2209	a shorter time frame	2190:2209	a shorter time frame	2190:2209	Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.					
36939098	3	81	theme	acid-chitosan-polyvinyl	359:381	arg1	nanofibers					399:408	hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers	348:408	hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility	348:446	In this regard, hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility were produced by optimizing the solution preparation process.					
36939098	4	82	theme	synthesized	527:537	arg1	nanofiber					549:557	the synthesized composite nanofiber	523:557	the synthesized composite nanofiber	523:557	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	13	83	theme	active	2001:2006	arg1	alternative					2037:2047	a good alternative	2030:2047	a good alternative to existing wound dressings	2030:2075	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	13	83	theme	active	2001:2006	arg1	mats					2018:2021	these active nanofiber mats	1995:2021	these active nanofiber mats	1995:2021	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	0	84	theme	Wound	89:93	arg1	Healing					95:101	Wound Healing	89:101	Wound Healing	89:101	Cross-Linked Electrospun pH-Sensitive Nanofibers Adsorbed with Temporin-Ra for Promoting Wound Healing.					
36939098	9	85	theme	Electrospun	1346:1356	arg1	nanofibers					1358:1367	Electrospun nanofibers	1346:1367	Electrospun nanofibers	1346:1367	Electrospun nanofibers can imitate the architecture of the extracellular matrix for accelerating wound healing.					
36939098	2	86	theme	delivery	314:321	arg1	systems					323:329	pH-sensitive drug delivery systems	296:329	pH-sensitive drug delivery systems	296:329	Poly-anionic and poly-cationic biopolymers have provided a superior platform for designing pH-sensitive drug delivery systems.					
36939098	1	87	theme	tissue	172:177	arg1	applications					191:202	tissue engineering applications	172:202	tissue engineering applications	172:202	Bioresponsive nanodrug delivery systems have excellent potential in tissue engineering applications.					
36939098	12	88	theme	%	1822:1822	arg1	closure					1830:1836	60% wound closure	1820:1836	60% wound closure	1820:1836	On day 6, the peptide-loaded nanofiber revealed 60% wound closure compared to the control group (17%).					
36939098	6	89	theme	positive	1067:1074	arg1	result					1027:1032	a result	1025:1032	a result	1025:1032	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	6	89	theme	positive	1067:1074	arg1	charge					1076:1081	a net positive charge	1061:1081	a net positive charge	1061:1081	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	4	90	theme	%	591:591	arg1	ratio					602:606	200% swelling ratio	588:606	200% swelling ratio	588:606	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	13	91	theme	tissue	1966:1971	arg1	repair					1973:1978	excellent tissue repair	1956:1978	excellent tissue repair ability	1956:1986	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	11	92	from	wounds	1647:1652	arg1	model					1667:1671	the mouse model	1657:1671	the mouse model	1657:1671	In vivo studies on full-thickness wounds in the mouse model indicated that the designed nanofiber was gradually absorbed without causing dryness or infection.					
36939098	10	93	theme	fibroblast	1535:1544	arg1	growth					1551:1556	fibroblast cell growth	1535:1556	fibroblast cell growth	1535:1556	In vitro investigation showed better adhesion, proliferation, migration, and fibroblast cell growth on peptide-loaded nanofiber samples than other groups.					
36939098	5	94	theme	prolonged	803:811	arg1	immersion					813:821	prolonged immersion	803:821	prolonged immersion in biological solutions	803:845	Green cross-linking with citric acid prevented the destruction of the nanofiber even after prolonged immersion in biological solutions.					
36939098	12	95	theme	peptide-loaded	1786:1799	arg1	nanofiber					1801:1809	the peptide-loaded nanofiber	1782:1809	the peptide-loaded nanofiber	1782:1809	On day 6, the peptide-loaded nanofiber revealed 60% wound closure compared to the control group (17%).					
36939098	4	96	theme	kN/mm	571:575	arg1	toughness					577:585	66.82 kN/mm toughness	565:585	66.82 kN/mm toughness	565:585	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	14	97	theme	shorter	2192:2198	arg1	frame					2205:2209	a shorter time frame	2190:2209	a shorter time frame	2190:2209	Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.					
36939098	13	98	theme	composite	1923:1931	arg1	nanofiber					1933:1941	the composite nanofiber	1919:1941	the composite nanofiber	1919:1941	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	4	99	theme	wound	696:700	arg1	dressing					702:709	the ideal wound dressing	686:709	the ideal wound dressing	686:709	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	10	100	theme	peptide-loaded	1561:1574	arg1	samples					1586:1592	peptide-loaded nanofiber samples	1561:1592	peptide-loaded nanofiber samples	1561:1592	In vitro investigation showed better adhesion, proliferation, migration, and fibroblast cell growth on peptide-loaded nanofiber samples than other groups.					
36939098	0	101	theme	Electrospun	13:23	arg1	Nanofibers					38:47	Cross-Linked Electrospun pH-Sensitive Nanofibers	0:47	Cross-Linked Electrospun pH-Sensitive Nanofibers	0:47	Cross-Linked Electrospun pH-Sensitive Nanofibers Adsorbed with Temporin-Ra for Promoting Wound Healing.					
36939098	9	102	theme	extracellular	1405:1417	arg1	matrix					1419:1424	the extracellular matrix	1401:1424	the extracellular matrix	1401:1424	Electrospun nanofibers can imitate the architecture of the extracellular matrix for accelerating wound healing.					
36939098	1	103	theme	Bioresponsive	104:116	arg1	systems					136:142	Bioresponsive nanodrug delivery systems	104:142	Bioresponsive nanodrug delivery systems	104:142	Bioresponsive nanodrug delivery systems have excellent potential in tissue engineering applications.					
36939098	3	104	theme	solution	480:487	arg1	process					501:507	the solution preparation process	476:507	the solution preparation process	476:507	In this regard, hyaluronic acid-chitosan-polyvinyl alcohol complex nanofibers with high quality and reproducibility were produced by optimizing the solution preparation process.					
36939098	14	105	theme	wound	2173:2177	arg1	healing					2179:2185	wound healing	2173:2185	wound healing	2173:2185	Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.					
36939098	13	106	theme	histological	1897:1908	arg1	studies					1910:1916	histological studies	1897:1916	histological studies	1897:1916	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	1	107	theme	delivery	127:134	arg1	systems					136:142	Bioresponsive nanodrug delivery systems	104:142	Bioresponsive nanodrug delivery systems	104:142	Bioresponsive nanodrug delivery systems have excellent potential in tissue engineering applications.					
36939098	4	108	theme	excellent	637:645	arg1	properties					647:656	excellent properties	637:656	excellent properties	637:656	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	10	109	theme	other	1599:1603	arg1	groups					1605:1610	other groups	1599:1610	other groups	1599:1610	In vitro investigation showed better adhesion, proliferation, migration, and fibroblast cell growth on peptide-loaded nanofiber samples than other groups.					
36939098	9	110	theme	wound	1443:1447	arg1	healing					1449:1455	wound healing	1443:1455	wound healing	1443:1455	Electrospun nanofibers can imitate the architecture of the extracellular matrix for accelerating wound healing.					
36939098	6	111	theme	potential	850:858	arg1	studies					860:866	ζ potential studies	848:866	ζ potential studies	848:866	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	4	112	theme	%	615:615	arg1	porosity					617:624	60% porosity	613:624	60% porosity	613:624	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
36939098	8	113	dep	has	1259:1261	arg1	bed					1255:1257	the wound bed	1245:1257	the wound bed	1245:1257	In vivo evaluation has proven that the wound bed has an alkaline environment, facilitating peptide release from the nanofiber scaffold.					
36939098	2	114	theme	superior	264:271	arg1	platform					273:280	a superior platform	262:280	a superior platform for designing pH-sensitive drug delivery systems	262:329	Poly-anionic and poly-cationic biopolymers have provided a superior platform for designing pH-sensitive drug delivery systems.					
36939098	14	115	theme	expression	2083:2092	arg1	studies					2094:2100	Gene expression studies	2078:2100	Gene expression studies	2078:2100	Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.					
36939098	13	116	theme	wound	2061:2065	arg1	dressings					2067:2075	existing wound dressings	2052:2075	existing wound dressings	2052:2075	In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings.					
36939098	8	117	theme	In	1210:1211	arg1	evaluation					1218:1227	In vivo evaluation	1210:1227	In vivo evaluation	1210:1227	In vivo evaluation has proven that the wound bed has an alkaline environment, facilitating peptide release from the nanofiber scaffold.					
36939098	6	118	theme	negative	918:925	arg1	charge					935:940	a negative surface charge	916:940	a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions	916:1109	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	6	118	theme	negative	918:925	arg1	∼-30					943:946	∼-30	943:946	∼-30	943:946	ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼-30) at physiological pH. The pKa of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions.					
36939098	4	119	theme	ideal	690:694	arg1	dressing					702:709	the ideal wound dressing	686:709	the ideal wound dressing	686:709	In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing.					
37239896	9	0	theme	adsorption/desorption	1400:1420	arg1	cycles					1422:1427	10 adsorption/desorption cycles	1397:1427	10 adsorption/desorption cycles	1397:1427	In particular, 10 adsorption/desorption cycles were performed, evidencing the great ability of this material that does not lose its efficiency.					
37239896	1	1	theme	chitosan-based	152:165	arg1	films					167:171	chitosan-based films	152:171	chitosan-based films blended with TiO2 (CH/TiO2)	152:199	In this work, the great performance of chitosan-based films blended with TiO2 (CH/TiO2) is presented to adsorb the hazardous pollutant 2,4-dinitrophenol (DNP) from water.					
37239896	1	2	theme	pollutant	238:246	arg1	2,4-dinitrophenol					248:264	the hazardous pollutant 2,4-dinitrophenol	224:264	the hazardous pollutant 2,4-dinitrophenol (DNP) from water	224:281	In this work, the great performance of chitosan-based films blended with TiO2 (CH/TiO2) is presented to adsorb the hazardous pollutant 2,4-dinitrophenol (DNP) from water.					
37239896	1	2	theme	pollutant	238:246	arg1	DNP					267:269	DNP	267:269	DNP	267:269	In this work, the great performance of chitosan-based films blended with TiO2 (CH/TiO2) is presented to adsorb the hazardous pollutant 2,4-dinitrophenol (DNP) from water.					
37239896	7	3	theme	adsorbent	1141:1149	arg1	regeneration					1151:1162	the adsorbent regeneration	1137:1162	the adsorbent regeneration	1137:1162	Finally, the adsorbent regeneration was exploited, and the possibility of inducing DNP desorption was investigated.					
37239896	3	4	theme	powerful	473:480	arg1	tool					482:485	a powerful tool	471:485	a powerful tool for monitoring the presence of DNP in purposely contaminated water	471:552	For pursuing the proposed aim, UV-Vis spectroscopy was considered a powerful tool for monitoring the presence of DNP in purposely contaminated water.					
37239896	3	5	from	presence	506:513	arg1	water					548:552	purposely contaminated water	525:552	purposely contaminated water	525:552	For pursuing the proposed aim, UV-Vis spectroscopy was considered a powerful tool for monitoring the presence of DNP in purposely contaminated water.					
37239896	6	6	theme	Weber-Morris	1108:1119	arg1	model					1121:1125	the Weber-Morris model	1104:1125	the Weber-Morris model	1104:1125	The applicability of pseudo-first- and pseudo-second-order kinetic equations confirmed the finding, further detailed by the Weber-Morris model.					
37239896	4	7	theme	adsorption	752:761	arg1	measurements					763:774	adsorption measurements	752:774	adsorption measurements	752:774	Swelling measurements were employed to infer more information about the interactions between chitosan and DNP, demonstrating the presence of electrostatic forces, deeply investigated by performing adsorption measurements by changing DNP solutions' ionic strength and pH values.					
37239896	5	8	theme	heterogeneous	939:951	arg1	character					953:961	the DNP adsorption's heterogeneous character	918:961	the DNP adsorption's heterogeneous character onto chitosan films	918:981	The thermodynamics, adsorption isotherms, and kinetics were also studied, suggesting the DNP adsorption's heterogeneous character onto chitosan films.					
37239896	1	9	theme	films	167:171	arg1	performance					137:147	the great performance	127:147	the great performance of chitosan-based films blended with TiO2 (CH/TiO2)	127:199	In this work, the great performance of chitosan-based films blended with TiO2 (CH/TiO2) is presented to adsorb the hazardous pollutant 2,4-dinitrophenol (DNP) from water.					
37239896	10	10	theme	Advanced	1594:1601	arg1	Processes					1613:1621	Advanced Oxidation Processes	1594:1621	Advanced Oxidation Processes	1594:1621	As an alternative approach, the pollutant photodegradation by using Advanced Oxidation Processes, allowed by the presence of TiO2, was preliminary investigated, opening a novel horizon in the use of chitosan-based materials for environmental applications.					
37239896	4	11	dep	demonstrating	666:678	arg1	investigated					725:736	investigated	725:736	investigated by performing adsorption measurements by changing DNP solutions' ionic strength and pH values	725:830	Swelling measurements were employed to infer more information about the interactions between chitosan and DNP, demonstrating the presence of electrostatic forces, deeply investigated by performing adsorption measurements by changing DNP solutions' ionic strength and pH values.					
37239896	2	12	theme	adsorption	330:339	arg1	%					341:341	a high adsorption %	323:341	a high adsorption %: CH/TiO2	323:350	The DNP was successfully removed, with a high adsorption %: CH/TiO2 exhibited a maximum adsorption capacity of 900 mg/g.					
37239896	4	13	theme	Swelling	555:562	arg1	measurements					564:575	Swelling measurements	555:575	Swelling measurements	555:575	Swelling measurements were employed to infer more information about the interactions between chitosan and DNP, demonstrating the presence of electrostatic forces, deeply investigated by performing adsorption measurements by changing DNP solutions' ionic strength and pH values.					
37239896	1	14	from	water	277:281	arg1	2,4-dinitrophenol					248:264	the hazardous pollutant 2,4-dinitrophenol	224:264	the hazardous pollutant 2,4-dinitrophenol (DNP) from water	224:281	In this work, the great performance of chitosan-based films blended with TiO2 (CH/TiO2) is presented to adsorb the hazardous pollutant 2,4-dinitrophenol (DNP) from water.					
37239896	1	14	from	water	277:281	arg1	DNP					267:269	DNP	267:269	DNP	267:269	In this work, the great performance of chitosan-based films blended with TiO2 (CH/TiO2) is presented to adsorb the hazardous pollutant 2,4-dinitrophenol (DNP) from water.					
37239896	5	15	theme	chitosan	968:975	arg1	films					977:981	chitosan films	968:981	chitosan films	968:981	The thermodynamics, adsorption isotherms, and kinetics were also studied, suggesting the DNP adsorption's heterogeneous character onto chitosan films.					
37239896	2	16	theme	maximum	364:370	arg1	capacity					383:390	a maximum adsorption capacity	362:390	a maximum adsorption capacity of 900 mg/g	362:402	The DNP was successfully removed, with a high adsorption %: CH/TiO2 exhibited a maximum adsorption capacity of 900 mg/g.					
37239896	7	17	theme	DNP	1211:1213	arg1	desorption					1215:1224	DNP desorption	1211:1224	DNP desorption	1211:1224	Finally, the adsorbent regeneration was exploited, and the possibility of inducing DNP desorption was investigated.					
37239896	10	18	theme	TiO2	1651:1654	arg1	presence					1639:1646	the presence	1635:1646	the presence of TiO2	1635:1654	As an alternative approach, the pollutant photodegradation by using Advanced Oxidation Processes, allowed by the presence of TiO2, was preliminary investigated, opening a novel horizon in the use of chitosan-based materials for environmental applications.					
37239896	2	19	dep	%	341:341	arg1	CH/TiO2					344:350	CH/TiO2	344:350	a high adsorption %: CH/TiO2	323:350	The DNP was successfully removed, with a high adsorption %: CH/TiO2 exhibited a maximum adsorption capacity of 900 mg/g.					
37239896	5	20	theme	adsorption	853:862	arg1	isotherms					864:872	adsorption isotherms	853:872	adsorption isotherms	853:872	The thermodynamics, adsorption isotherms, and kinetics were also studied, suggesting the DNP adsorption's heterogeneous character onto chitosan films.					
37239896	0	21	theme	Adsorption	4:13	arg1	Efficiency					15:24	The Adsorption Efficiency	0:24	The Adsorption Efficiency of Regenerable Chitosan-TiO2 Composite Films in Removing 2,4-Dinitrophenol from Water.	0:111	The Adsorption Efficiency of Regenerable Chitosan-TiO2 Composite Films in Removing 2,4-Dinitrophenol from Water.					
37239896	5	22	theme	DNP	922:924	arg1	character					953:961	the DNP adsorption's heterogeneous character	918:961	the DNP adsorption's heterogeneous character onto chitosan films	918:981	The thermodynamics, adsorption isotherms, and kinetics were also studied, suggesting the DNP adsorption's heterogeneous character onto chitosan films.					
37239896	4	23	theme	electrostatic	696:708	arg1	forces					710:715	electrostatic forces	696:715	electrostatic forces	696:715	Swelling measurements were employed to infer more information about the interactions between chitosan and DNP, demonstrating the presence of electrostatic forces, deeply investigated by performing adsorption measurements by changing DNP solutions' ionic strength and pH values.					
37239896	3	24	theme	contaminated	535:546	arg1	water					548:552	purposely contaminated water	525:552	purposely contaminated water	525:552	For pursuing the proposed aim, UV-Vis spectroscopy was considered a powerful tool for monitoring the presence of DNP in purposely contaminated water.					
37239896	8	25	theme	suitable	1262:1269	arg1	experiments					1271:1281	suitable experiments	1262:1281	suitable experiments	1262:1281	For this purpose, suitable experiments were conducted using a saline solution that induced the DNP release, favoring the adsorbent reuse.					
37239896	9	26	theme	great	1460:1464	arg1	ability					1466:1472	the great ability	1456:1472	the great ability of this material that does not lose its efficiency	1456:1523	In particular, 10 adsorption/desorption cycles were performed, evidencing the great ability of this material that does not lose its efficiency.					
37239896	3	27	theme	DNP	518:520	arg1	presence					506:513	the presence	502:513	the presence of DNP in purposely contaminated water	502:552	For pursuing the proposed aim, UV-Vis spectroscopy was considered a powerful tool for monitoring the presence of DNP in purposely contaminated water.					
37239896	3	28	attach	presence	506:513	arg1	water					548:552	purposely contaminated water	525:552	purposely contaminated water	525:552	For pursuing the proposed aim, UV-Vis spectroscopy was considered a powerful tool for monitoring the presence of DNP in purposely contaminated water.					
37239896	3	28	attach	presence	506:513	arg2	DNP					518:520	DNP	518:520	DNP	518:520	For pursuing the proposed aim, UV-Vis spectroscopy was considered a powerful tool for monitoring the presence of DNP in purposely contaminated water.					
37239896	9	29	theme	material	1482:1489	arg1	ability					1466:1472	the great ability	1456:1472	the great ability of this material that does not lose its efficiency	1456:1523	In particular, 10 adsorption/desorption cycles were performed, evidencing the great ability of this material that does not lose its efficiency.					
37239896	2	30	theme	mg/g	399:402	arg1	capacity					383:390	a maximum adsorption capacity	362:390	a maximum adsorption capacity of 900 mg/g	362:402	The DNP was successfully removed, with a high adsorption %: CH/TiO2 exhibited a maximum adsorption capacity of 900 mg/g.					
37239896	2	31	theme	high	325:328	arg1	%					341:341	a high adsorption %	323:341	a high adsorption %: CH/TiO2	323:350	The DNP was successfully removed, with a high adsorption %: CH/TiO2 exhibited a maximum adsorption capacity of 900 mg/g.					
37239896	10	32	theme	chitosan-based	1725:1738	arg1	materials					1740:1748	chitosan-based materials	1725:1748	chitosan-based materials for environmental applications	1725:1779	As an alternative approach, the pollutant photodegradation by using Advanced Oxidation Processes, allowed by the presence of TiO2, was preliminary investigated, opening a novel horizon in the use of chitosan-based materials for environmental applications.					
37239896	4	33	theme	more	600:603	arg1	information					605:615	more information	600:615	more information about the interactions between chitosan and DNP	600:663	Swelling measurements were employed to infer more information about the interactions between chitosan and DNP, demonstrating the presence of electrostatic forces, deeply investigated by performing adsorption measurements by changing DNP solutions' ionic strength and pH values.					
37239896	0	34	theme	Chitosan-TiO2	41:53	arg1	Films					65:69	Regenerable Chitosan-TiO2 Composite Films	29:69	Regenerable Chitosan-TiO2 Composite Films	29:69	The Adsorption Efficiency of Regenerable Chitosan-TiO2 Composite Films in Removing 2,4-Dinitrophenol from Water.					
37239896	0	35	theme	Regenerable	29:39	arg1	Films					65:69	Regenerable Chitosan-TiO2 Composite Films	29:69	Regenerable Chitosan-TiO2 Composite Films	29:69	The Adsorption Efficiency of Regenerable Chitosan-TiO2 Composite Films in Removing 2,4-Dinitrophenol from Water.					
37239896	4	36	theme	ionic	803:807	arg1	strength					809:816	DNP solutions' ionic strength	788:816	DNP solutions' ionic strength	788:816	Swelling measurements were employed to infer more information about the interactions between chitosan and DNP, demonstrating the presence of electrostatic forces, deeply investigated by performing adsorption measurements by changing DNP solutions' ionic strength and pH values.					
37239896	2	37	theme	adsorption	372:381	arg1	capacity					383:390	a maximum adsorption capacity	362:390	a maximum adsorption capacity of 900 mg/g	362:402	The DNP was successfully removed, with a high adsorption %: CH/TiO2 exhibited a maximum adsorption capacity of 900 mg/g.					
37239896	0	38	theme	Films	65:69	arg1	Efficiency					15:24	The Adsorption Efficiency	0:24	The Adsorption Efficiency of Regenerable Chitosan-TiO2 Composite Films in Removing 2,4-Dinitrophenol from Water.	0:111	The Adsorption Efficiency of Regenerable Chitosan-TiO2 Composite Films in Removing 2,4-Dinitrophenol from Water.					
37239896	10	39	theme	Oxidation	1603:1611	arg1	Processes					1613:1621	Advanced Oxidation Processes	1594:1621	Advanced Oxidation Processes	1594:1621	As an alternative approach, the pollutant photodegradation by using Advanced Oxidation Processes, allowed by the presence of TiO2, was preliminary investigated, opening a novel horizon in the use of chitosan-based materials for environmental applications.					
37239896	6	40	theme	equations	1051:1059	arg1	applicability					988:1000	The applicability	984:1000	The applicability of pseudo-first- and pseudo-second-order kinetic equations	984:1059	The applicability of pseudo-first- and pseudo-second-order kinetic equations confirmed the finding, further detailed by the Weber-Morris model.					
37239896	10	41	theme	pollutant	1558:1566	arg1	photodegradation					1568:1583	the pollutant photodegradation	1554:1583	the pollutant photodegradation	1554:1583	As an alternative approach, the pollutant photodegradation by using Advanced Oxidation Processes, allowed by the presence of TiO2, was preliminary investigated, opening a novel horizon in the use of chitosan-based materials for environmental applications.					
37239896	10	41	theme	pollutant	1558:1566	arg1	approach					1544:1551	an alternative approach	1529:1551	an alternative approach	1529:1551	As an alternative approach, the pollutant photodegradation by using Advanced Oxidation Processes, allowed by the presence of TiO2, was preliminary investigated, opening a novel horizon in the use of chitosan-based materials for environmental applications.					
37239896	4	42	theme	pH	822:823	arg1	values					825:830	pH values	822:830	pH values	822:830	Swelling measurements were employed to infer more information about the interactions between chitosan and DNP, demonstrating the presence of electrostatic forces, deeply investigated by performing adsorption measurements by changing DNP solutions' ionic strength and pH values.					
37239896	0	43	attach	Removing	74:81	arg1	Water					106:110	Water	106:110	Water	106:110	The Adsorption Efficiency of Regenerable Chitosan-TiO2 Composite Films in Removing 2,4-Dinitrophenol from Water.					
37239896	0	43	attach	Removing	74:81	arg2	Efficiency					15:24	The Adsorption Efficiency	0:24	The Adsorption Efficiency of Regenerable Chitosan-TiO2 Composite Films in Removing 2,4-Dinitrophenol from Water.	0:111	The Adsorption Efficiency of Regenerable Chitosan-TiO2 Composite Films in Removing 2,4-Dinitrophenol from Water.					
37239896	0	44	theme	Composite	55:63	arg1	Films					65:69	Regenerable Chitosan-TiO2 Composite Films	29:69	Regenerable Chitosan-TiO2 Composite Films	29:69	The Adsorption Efficiency of Regenerable Chitosan-TiO2 Composite Films in Removing 2,4-Dinitrophenol from Water.					
37239896	10	45	theme	alternative	1532:1542	arg1	photodegradation					1568:1583	the pollutant photodegradation	1554:1583	the pollutant photodegradation	1554:1583	As an alternative approach, the pollutant photodegradation by using Advanced Oxidation Processes, allowed by the presence of TiO2, was preliminary investigated, opening a novel horizon in the use of chitosan-based materials for environmental applications.					
37239896	10	45	theme	alternative	1532:1542	arg1	approach					1544:1551	an alternative approach	1529:1551	an alternative approach	1529:1551	As an alternative approach, the pollutant photodegradation by using Advanced Oxidation Processes, allowed by the presence of TiO2, was preliminary investigated, opening a novel horizon in the use of chitosan-based materials for environmental applications.					
37239896	8	46	theme	saline	1306:1311	arg1	solution					1313:1320	a saline solution	1304:1320	a saline solution that induced the DNP release, favoring the adsorbent reuse	1304:1379	For this purpose, suitable experiments were conducted using a saline solution that induced the DNP release, favoring the adsorbent reuse.					
37239896	4	47	theme	DNP	788:790	arg1	strength					809:816	DNP solutions' ionic strength	788:816	DNP solutions' ionic strength	788:816	Swelling measurements were employed to infer more information about the interactions between chitosan and DNP, demonstrating the presence of electrostatic forces, deeply investigated by performing adsorption measurements by changing DNP solutions' ionic strength and pH values.					
37239896	6	48	theme	pseudo-second-order	1023:1041	arg1	equations					1051:1059	pseudo-first- and pseudo-second-order kinetic equations	1005:1059	pseudo-first- and pseudo-second-order kinetic equations	1005:1059	The applicability of pseudo-first- and pseudo-second-order kinetic equations confirmed the finding, further detailed by the Weber-Morris model.					
37239896	3	49	theme	UV-Vis	436:441	arg1	spectroscopy					443:454	UV-Vis spectroscopy	436:454	UV-Vis spectroscopy	436:454	For pursuing the proposed aim, UV-Vis spectroscopy was considered a powerful tool for monitoring the presence of DNP in purposely contaminated water.					
37239896	6	50	theme	kinetic	1043:1049	arg1	equations					1051:1059	pseudo-first- and pseudo-second-order kinetic equations	1005:1059	pseudo-first- and pseudo-second-order kinetic equations	1005:1059	The applicability of pseudo-first- and pseudo-second-order kinetic equations confirmed the finding, further detailed by the Weber-Morris model.					
37239896	8	51	theme	adsorbent	1365:1373	arg1	reuse					1375:1379	the adsorbent reuse	1361:1379	the adsorbent reuse	1361:1379	For this purpose, suitable experiments were conducted using a saline solution that induced the DNP release, favoring the adsorbent reuse.					
37239896	10	52	from	opening	1687:1693	arg1	use					1718:1720	the use	1714:1720	the use of chitosan-based materials for environmental applications	1714:1779	As an alternative approach, the pollutant photodegradation by using Advanced Oxidation Processes, allowed by the presence of TiO2, was preliminary investigated, opening a novel horizon in the use of chitosan-based materials for environmental applications.					
37239896	1	53	theme	great	131:135	arg1	performance					137:147	the great performance	127:147	the great performance of chitosan-based films blended with TiO2 (CH/TiO2)	127:199	In this work, the great performance of chitosan-based films blended with TiO2 (CH/TiO2) is presented to adsorb the hazardous pollutant 2,4-dinitrophenol (DNP) from water.					
37239896	4	54	theme	forces	710:715	arg1	presence					684:691	the presence	680:691	the presence of electrostatic forces	680:715	Swelling measurements were employed to infer more information about the interactions between chitosan and DNP, demonstrating the presence of electrostatic forces, deeply investigated by performing adsorption measurements by changing DNP solutions' ionic strength and pH values.					
37239896	3	55	theme	proposed	422:429	arg1	aim					431:433	the proposed aim	418:433	the proposed aim	418:433	For pursuing the proposed aim, UV-Vis spectroscopy was considered a powerful tool for monitoring the presence of DNP in purposely contaminated water.					
37239896	10	56	theme	novel	1697:1701	arg1	horizon					1703:1709	a novel horizon	1695:1709	a novel horizon	1695:1709	As an alternative approach, the pollutant photodegradation by using Advanced Oxidation Processes, allowed by the presence of TiO2, was preliminary investigated, opening a novel horizon in the use of chitosan-based materials for environmental applications.					
37239896	8	57	theme	DNP	1339:1341	arg1	release					1343:1349	the DNP release	1335:1349	the DNP release	1335:1349	For this purpose, suitable experiments were conducted using a saline solution that induced the DNP release, favoring the adsorbent reuse.					
37239896	10	58	theme	environmental	1754:1766	arg1	applications					1768:1779	environmental applications	1754:1779	environmental applications	1754:1779	As an alternative approach, the pollutant photodegradation by using Advanced Oxidation Processes, allowed by the presence of TiO2, was preliminary investigated, opening a novel horizon in the use of chitosan-based materials for environmental applications.					
37239896	6	59	theme	pseudo-first-	1005:1017	arg1	equations					1051:1059	pseudo-first- and pseudo-second-order kinetic equations	1005:1059	pseudo-first- and pseudo-second-order kinetic equations	1005:1059	The applicability of pseudo-first- and pseudo-second-order kinetic equations confirmed the finding, further detailed by the Weber-Morris model.					
37239896	10	60	theme	materials	1740:1748	arg1	use					1718:1720	the use	1714:1720	the use of chitosan-based materials for environmental applications	1714:1779	As an alternative approach, the pollutant photodegradation by using Advanced Oxidation Processes, allowed by the presence of TiO2, was preliminary investigated, opening a novel horizon in the use of chitosan-based materials for environmental applications.					
37239896	1	61	theme	hazardous	228:236	arg1	2,4-dinitrophenol					248:264	the hazardous pollutant 2,4-dinitrophenol	224:264	the hazardous pollutant 2,4-dinitrophenol (DNP) from water	224:281	In this work, the great performance of chitosan-based films blended with TiO2 (CH/TiO2) is presented to adsorb the hazardous pollutant 2,4-dinitrophenol (DNP) from water.					
37239896	1	61	theme	hazardous	228:236	arg1	DNP					267:269	DNP	267:269	DNP	267:269	In this work, the great performance of chitosan-based films blended with TiO2 (CH/TiO2) is presented to adsorb the hazardous pollutant 2,4-dinitrophenol (DNP) from water.					
36979573	4	0	theme	one-step	654:661	arg1	method					663:668	a one-step method	652:668	a one-step method	652:668	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	8	1	theme	environmental	1280:1292	arg1	samples					1300:1306	environmental water samples	1280:1306	environmental water samples	1280:1306	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	1	2	from	variety	254:260	arg1	processes					303:311	biological processes	292:311	biological processes	292:311	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	8	3	theme	red-emitting	1353:1364	arg1	nanoclusters					1373:1384	red-emitting copper nanoclusters	1353:1384	red-emitting copper nanoclusters (Cu NCs)	1353:1393	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	8	3	theme	red-emitting	1353:1364	arg1	NCs					1390:1392	Cu NCs	1387:1392	Cu NCs	1387:1392	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	4	4	theme	protective	719:728	arg1	ligands					730:736	protective ligands	719:736	protective ligands	719:736	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	4	4	theme	protective	719:728	arg1	nanoclusters					627:638	the red-emitting copper nanoclusters	603:638	the red-emitting copper nanoclusters (Cu NCs)	603:647	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	8	5	theme	water	1294:1298	arg1	samples					1300:1306	environmental water samples	1280:1306	environmental water samples	1280:1306	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	1	6	theme	essential	327:335	arg1	it					318:319	it	318:319	it	318:319	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	1	6	theme	essential	327:335	arg1	mediator					337:344	an essential mediator	324:344	an essential mediator in food, pharmaceutical, and environmental analysis	324:396	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	3	7	theme	H2O2	512:515	arg1	concentration					495:507	the concentration	491:507	the concentration of H2O2 in the environment for human health and environmental protection	491:580	It is crucial to detect the concentration of H2O2 in the environment for human health and environmental protection.					
36979573	8	8	theme	fluorescent	1204:1214	arg1	probe					1216:1220	the as--developed fluorescent probe	1186:1220	the as--developed fluorescent probe	1186:1220	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	1	9	from	processes	303:311	arg1	functions					279:287	physiological functions	265:287	physiological functions in biological processes	265:311	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	1	9	from	processes	303:311	arg1	variety					254:260	a variety	252:260	a variety of physiological functions in biological processes	252:311	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	7	10	theme	H2O2	1089:1092	arg1	detection					1076:1084	the detection	1072:1084	the detection of H2O2 with a limit of detection of 47 nM	1072:1127	Based on this, a fluorescent probe based on LA-Cu NCs@CS was constructed for the detection of H2O2 with a limit of detection of 47 nM.					
36979573	8	11	from	sensitivity	1257:1267	arg1	samples					1300:1306	environmental water samples	1280:1306	environmental water samples	1280:1306	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	7	12	theme	detection	1110:1118	arg1	limit					1101:1105	a limit	1099:1105	a limit of detection of 47 nM	1099:1127	Based on this, a fluorescent probe based on LA-Cu NCs@CS was constructed for the detection of H2O2 with a limit of detection of 47 nM.					
36979573	0	13	theme	Peroxide	114:121	arg1	Probe					133:137	a Hydrogen Peroxide Detection Probe	103:137	a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples	103:170	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite and Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples.					
36979573	8	14	theme	copper	1366:1371	arg1	nanoclusters					1373:1384	red-emitting copper nanoclusters	1353:1384	red-emitting copper nanoclusters (Cu NCs)	1353:1393	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	8	14	theme	copper	1366:1371	arg1	NCs					1390:1392	Cu NCs	1387:1392	Cu NCs	1387:1392	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	1	15	from	functions	279:287	arg1	processes					303:311	biological processes	292:311	biological processes	292:311	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	5	16	theme	LA-Cu	884:888	arg1	NCs					890:892	LA-Cu NCs	884:892	LA-Cu NCs	884:892	The as-prepared LA-Cu NCs@CS have stronger fluorescence than LA-Cu NCs.					
36979573	5	17	theme	LA-Cu	839:843	arg1	CS					849:850	The as-prepared LA-Cu NCs@CS	823:850	The as-prepared LA-Cu NCs@CS	823:850	The as-prepared LA-Cu NCs@CS have stronger fluorescence than LA-Cu NCs.					
36979573	6	18	theme	CS	967:968	arg1	fluorescence					941:952	the fluorescence	937:952	the fluorescence of LA-Cu NCs@CS	937:968	We found that the presence of H2O2 causes the fluorescence of LA-Cu NCs@CS to be strongly quenched.					
36979573	0	19	theme	Hydrogen	105:112	arg1	Peroxide					114:121	Hydrogen Peroxide	105:121	a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples	103:170	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite and Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples.					
36979573	1	20	theme	food	349:352	arg1	analysis					389:396	food, pharmaceutical, and environmental analysis	349:396	food, pharmaceutical, and environmental analysis	349:396	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	7	21	theme	fluorescent	1012:1022	arg1	probe					1024:1028	a fluorescent probe	1010:1028	a fluorescent probe based on LA-Cu NCs@CS	1010:1050	Based on this, a fluorescent probe based on LA-Cu NCs@CS was constructed for the detection of H2O2 with a limit of detection of 47 nM.					
36979573	7	22	with	detection	1076:1084	arg1	limit					1101:1105	a limit	1099:1105	a limit of detection of 47 nM	1099:1127	Based on this, a fluorescent probe based on LA-Cu NCs@CS was constructed for the detection of H2O2 with a limit of detection of 47 nM.					
36979573	8	23	from	selectivity	1236:1246	arg1	samples					1300:1306	environmental water samples	1280:1306	environmental water samples	1280:1306	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	1	24	theme	Hydrogen	173:180	arg1	peroxide					182:189	Hydrogen peroxide	173:189	Hydrogen peroxide (H2O2)	173:196	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	1	24	theme	Hydrogen	173:180	arg1	species					230:236	an important reactive oxygen species	201:236	an important reactive oxygen species that mediates a variety of physiological functions in biological processes	201:311	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	1	24	theme	Hydrogen	173:180	arg1	H2O2					192:195	H2O2	192:195	H2O2	192:195	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	7	25	theme	LA-Cu	1039:1043	arg1	CS					1049:1050	LA-Cu NCs@CS	1039:1050	LA-Cu NCs@CS	1039:1050	Based on this, a fluorescent probe based on LA-Cu NCs@CS was constructed for the detection of H2O2 with a limit of detection of 47 nM.					
36979573	7	26	theme	nM	1126:1127	arg1	detection					1110:1118	detection	1110:1118	detection of 47 nM	1110:1127	Based on this, a fluorescent probe based on LA-Cu NCs@CS was constructed for the detection of H2O2 with a limit of detection of 47 nM.					
36979573	8	27	from	research	1152:1159	arg1	results					1134:1140	The results	1130:1140	The results from this research	1130:1159	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	5	28	theme	@	848:848	arg1	CS					849:850	The as-prepared LA-Cu NCs@CS	823:850	The as-prepared LA-Cu NCs@CS	823:850	The as-prepared LA-Cu NCs@CS have stronger fluorescence than LA-Cu NCs.					
36979573	6	29	theme	NCs	963:965	arg1	CS					967:968	LA-Cu NCs@CS	957:968	LA-Cu NCs@CS	957:968	We found that the presence of H2O2 causes the fluorescence of LA-Cu NCs@CS to be strongly quenched.					
36979573	7	30	theme	NCs	1045:1047	arg1	CS					1049:1050	LA-Cu NCs@CS	1039:1050	LA-Cu NCs@CS	1039:1050	Based on this, a fluorescent probe based on LA-Cu NCs@CS was constructed for the detection of H2O2 with a limit of detection of 47 nM.					
36979573	1	31	from	mediator	337:344	arg1	analysis					389:396	food, pharmaceutical, and environmental analysis	349:396	food, pharmaceutical, and environmental analysis	349:396	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	3	32	theme	human	540:544	arg1	health					546:551	human health	540:551	human health	540:551	It is crucial to detect the concentration of H2O2 in the environment for human health and environmental protection.					
36979573	4	33	theme	Cu	641:642	arg1	nanoclusters					627:638	the red-emitting copper nanoclusters	603:638	the red-emitting copper nanoclusters (Cu NCs)	603:647	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	4	33	theme	Cu	641:642	arg1	NCs					644:646	Cu NCs	641:646	Cu NCs	641:646	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	0	34	theme	Red-Emitting	17:28	arg1	Composite					70:78	a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite	15:78	a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite	15:78	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite and Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples.					
36979573	1	35	theme	physiological	265:277	arg1	functions					279:287	physiological functions	265:287	physiological functions in biological processes	265:311	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	5	36	contain	have	852:855	arg1	CS					849:850	The as-prepared LA-Cu NCs@CS	823:850	The as-prepared LA-Cu NCs@CS	823:850	The as-prepared LA-Cu NCs@CS have stronger fluorescence than LA-Cu NCs.					
36979573	5	36	contain	have	852:855	arg2	fluorescence					866:877	stronger fluorescence	857:877	stronger fluorescence	857:877	The as-prepared LA-Cu NCs@CS have stronger fluorescence than LA-Cu NCs.					
36979573	8	37	theme	as--developed	1190:1202	arg1	probe					1216:1220	the as--developed fluorescent probe	1186:1220	the as--developed fluorescent probe	1186:1220	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	1	38	theme	functions	279:287	arg1	functions					279:287	physiological functions	265:287	physiological functions in biological processes	265:311	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	1	38	theme	functions	279:287	arg1	variety					254:260	a variety	252:260	a variety of physiological functions in biological processes	252:311	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	0	39	theme	Detection	123:131	arg1	Probe					133:137	a Hydrogen Peroxide Detection Probe	103:137	a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples	103:170	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite and Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples.					
36979573	1	40	theme	pharmaceutical	355:368	arg1	analysis					389:396	food, pharmaceutical, and environmental analysis	349:396	food, pharmaceutical, and environmental analysis	349:396	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	5	41	theme	stronger	857:864	arg1	fluorescence					866:877	stronger fluorescence	857:877	stronger fluorescence	857:877	The as-prepared LA-Cu NCs@CS have stronger fluorescence than LA-Cu NCs.					
36979573	2	42	theme	certain	443:449	arg1	concentrations					451:464	certain concentrations	443:464	certain concentrations	443:464	However, H2O2 can be dangerous and toxic at certain concentrations.					
36979573	2	43	from	concentrations	451:464	arg1	dangerous					420:428	dangerous	420:428	dangerous	420:428	However, H2O2 can be dangerous and toxic at certain concentrations.					
36979573	3	44	theme	environmental	557:569	arg1	protection					571:580	environmental protection	557:580	environmental protection	557:580	It is crucial to detect the concentration of H2O2 in the environment for human health and environmental protection.					
36979573	6	45	theme	LA-Cu	957:961	arg1	CS					967:968	LA-Cu NCs@CS	957:968	LA-Cu NCs@CS	957:968	We found that the presence of H2O2 causes the fluorescence of LA-Cu NCs@CS to be strongly quenched.					
36979573	4	46	theme	copper	620:625	arg1	ligands					730:736	protective ligands	719:736	protective ligands	719:736	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	4	46	theme	copper	620:625	arg1	agents					751:756	reducing agents	742:756	reducing agents	742:756	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	4	46	theme	copper	620:625	arg1	nanoclusters					627:638	the red-emitting copper nanoclusters	603:638	the red-emitting copper nanoclusters (Cu NCs)	603:647	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	4	46	theme	copper	620:625	arg1	NCs					644:646	Cu NCs	641:646	Cu NCs	641:646	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	1	47	theme	biological	292:301	arg1	processes					303:311	biological processes	292:311	biological processes	292:311	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	0	48	from	Probe	133:137	arg1	Analysis					146:153	the Analysis	142:153	the Analysis of Water Samples	142:170	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite and Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples.					
36979573	4	49	theme	red-emitting	607:618	arg1	ligands					730:736	protective ligands	719:736	protective ligands	719:736	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	4	49	theme	red-emitting	607:618	arg1	agents					751:756	reducing agents	742:756	reducing agents	742:756	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	4	49	theme	red-emitting	607:618	arg1	nanoclusters					627:638	the red-emitting copper nanoclusters	603:638	the red-emitting copper nanoclusters (Cu NCs)	603:647	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	4	49	theme	red-emitting	607:618	arg1	NCs					644:646	Cu NCs	641:646	Cu NCs	641:646	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	0	50	theme	Nanocluster	58:68	arg1	Composite					70:78	a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite	15:78	a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite	15:78	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite and Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples.					
36979573	8	51	theme	one-step	1400:1407	arg1	method					1409:1414	a one-step method	1398:1414	a one-step method	1398:1414	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	4	52	theme	LA-Cu	812:816	arg1	NCs					818:820	LA-Cu NCs	812:820	LA-Cu NCs	812:820	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	1	53	theme	environmental	375:387	arg1	analysis					389:396	food, pharmaceutical, and environmental analysis	349:396	food, pharmaceutical, and environmental analysis	349:396	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	8	54	theme	high	1252:1255	arg1	sensitivity					1257:1267	high sensitivity	1252:1267	high sensitivity to H2O2 in environmental water samples	1252:1306	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	8	55	theme	Cu	1387:1388	arg1	nanoclusters					1373:1384	red-emitting copper nanoclusters	1353:1384	red-emitting copper nanoclusters (Cu NCs)	1353:1393	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	8	55	theme	Cu	1387:1388	arg1	NCs					1390:1392	Cu NCs	1387:1392	Cu NCs	1387:1392	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	0	56	theme	Copper	51:56	arg1	Composite					70:78	a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite	15:78	a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite	15:78	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite and Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples.					
36979573	1	57	from	species	230:236	arg1	analysis					389:396	food, pharmaceutical, and environmental analysis	349:396	food, pharmaceutical, and environmental analysis	349:396	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	5	58	theme	as-prepared	827:837	arg1	CS					849:850	The as-prepared LA-Cu NCs@CS	823:850	The as-prepared LA-Cu NCs@CS	823:850	The as-prepared LA-Cu NCs@CS have stronger fluorescence than LA-Cu NCs.					
36979573	0	59	theme	Samples	164:170	arg1	Analysis					146:153	the Analysis	142:153	the Analysis of Water Samples	142:170	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite and Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples.					
36979573	0	60	dep	Red-Emitting	17:28	arg1	Chitosan-Stabilized					31:49	Chitosan-Stabilized	31:49	Chitosan-Stabilized	31:49	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite and Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples.					
36979573	8	61	theme	good	1231:1234	arg1	selectivity					1236:1246	good selectivity	1231:1246	good selectivity	1231:1246	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	4	62	theme	lipoic	676:681	arg1	LA					689:690	LA	689:690	LA	689:690	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	4	62	theme	lipoic	676:681	arg1	acid					683:686	lipoic acid	676:686	lipoic acid (LA)	676:691	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	1	63	theme	important	204:212	arg1	peroxide					182:189	Hydrogen peroxide	173:189	Hydrogen peroxide (H2O2)	173:196	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	1	63	theme	important	204:212	arg1	species					230:236	an important reactive oxygen species	201:236	an important reactive oxygen species that mediates a variety of physiological functions in biological processes	201:311	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	0	64	theme	Water	158:162	arg1	Samples					164:170	Water Samples	158:170	Water Samples	158:170	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite and Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples.					
36979573	8	65	theme	novel	1327:1331	arg1	strategy					1333:1340	a novel strategy	1325:1340	a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method	1325:1414	The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to H2O2 in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.					
36979573	4	66	theme	sodium	697:702	arg1	borohydride					704:714	sodium borohydride	697:714	sodium borohydride	697:714	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	5	67	theme	NCs	845:847	arg1	CS					849:850	The as-prepared LA-Cu NCs@CS	823:850	The as-prepared LA-Cu NCs@CS	823:850	The as-prepared LA-Cu NCs@CS have stronger fluorescence than LA-Cu NCs.					
36979573	1	68	theme	reactive	214:221	arg1	peroxide					182:189	Hydrogen peroxide	173:189	Hydrogen peroxide (H2O2)	173:196	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	1	68	theme	reactive	214:221	arg1	species					230:236	an important reactive oxygen species	201:236	an important reactive oxygen species that mediates a variety of physiological functions in biological processes	201:311	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	4	69	theme	reducing	742:749	arg1	nanoclusters					627:638	the red-emitting copper nanoclusters	603:638	the red-emitting copper nanoclusters (Cu NCs)	603:647	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	4	69	theme	reducing	742:749	arg1	agents					751:756	reducing agents	742:756	reducing agents	742:756	Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs.					
36979573	6	70	theme	@	966:966	arg1	CS					967:968	LA-Cu NCs@CS	957:968	LA-Cu NCs@CS	957:968	We found that the presence of H2O2 causes the fluorescence of LA-Cu NCs@CS to be strongly quenched.					
36979573	1	71	theme	oxygen	223:228	arg1	peroxide					182:189	Hydrogen peroxide	173:189	Hydrogen peroxide (H2O2)	173:196	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	1	71	theme	oxygen	223:228	arg1	species					230:236	an important reactive oxygen species	201:236	an important reactive oxygen species that mediates a variety of physiological functions in biological processes	201:311	Hydrogen peroxide (H2O2) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis.					
36979573	0	72	theme	Composite	70:78	arg1	Preparation					0:10	Preparation	0:10	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite	0:78	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite and Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples.					
36979573	0	72	theme	Composite	70:78	arg1	Application					88:98	Its Application	84:98	Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples	84:170	Preparation of a Red-Emitting, Chitosan-Stabilized Copper Nanocluster Composite and Its Application as a Hydrogen Peroxide Detection Probe in the Analysis of Water Samples.					
36979573	7	73	theme	@	1048:1048	arg1	CS					1049:1050	LA-Cu NCs@CS	1039:1050	LA-Cu NCs@CS	1039:1050	Based on this, a fluorescent probe based on LA-Cu NCs@CS was constructed for the detection of H2O2 with a limit of detection of 47 nM.					
36979573	6	74	theme	H2O2	925:928	arg1	presence					913:920	the presence	909:920	the presence of H2O2	909:928	We found that the presence of H2O2 causes the fluorescence of LA-Cu NCs@CS to be strongly quenched.					
36979573	3	75	from	concentration	495:507	arg1	environment					524:534	the environment	520:534	the environment for human health and environmental protection	520:580	It is crucial to detect the concentration of H2O2 in the environment for human health and environmental protection.					
36371568	0	0	theme	aqueous	89:95	arg1	solution					97:104	aqueous solution	89:104	aqueous solution	89:104	Renewable cellulose aerogel embedded with nano-HFO for preferable phosphate capture from aqueous solution.					
36371568	6	1	theme	CA	873:874	arg1	capacity					857:864	The maximum adsorption capacity	834:864	The maximum adsorption capacity of HFO@CA	834:874	The maximum adsorption capacity of HFO@CA occurs at near-acidic pH. With increasing temperature, the composite adsorbent is more favorable for phosphate adsorption.					
36371568	10	2	theme	@	1432:1432	arg1	CA					1433:1434	HFO@CA	1429:1434	HFO@CA	1429:1434	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	9	3	theme	adsorption	1297:1306	arg1	experiments					1308:1318	the phosphate adsorption experiments	1283:1318	the phosphate adsorption experiments without obvious capacity loss	1283:1348	In addition, five cycles of the phosphate adsorption experiments without obvious capacity loss indicated that HFO@CA has great regenerability.					
36371568	10	4	from	prospects	1478:1486	arg1	wastewater					1514:1523	wastewater	1514:1523	wastewater	1514:1523	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	10	4	from	prospects	1478:1486	arg1	removal					1501:1507	phosphate removal	1491:1507	phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources	1491:1644	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	5	5	theme	Batch	728:732	arg1	experiments					745:755	Batch adsorption experiments	728:755	Batch adsorption experiments	728:755	Batch adsorption experiments were carried out to evaluate the phosphate uptake by the prepared adsorbent.					
36371568	6	6	theme	@	872:872	arg1	CA					873:874	HFO@CA	869:874	HFO@CA	869:874	The maximum adsorption capacity of HFO@CA occurs at near-acidic pH. With increasing temperature, the composite adsorbent is more favorable for phosphate adsorption.					
36371568	8	7	contain	containing	1207:1216	arg2	anions					1230:1235	competitive anions	1218:1235	competitive anions	1218:1235	HFO@CA shows excellent adsorption selectivity in solutions containing competitive anions at higher levels.					
36371568	8	7	contain	containing	1207:1216	arg1	solutions					1197:1205	solutions	1197:1205	solutions containing competitive anions at higher levels	1197:1252	HFO@CA shows excellent adsorption selectivity in solutions containing competitive anions at higher levels.					
36371568	3	8	theme	CA	495:496	arg1	network					499:505	a cellulose aerogel (CA) network	474:505	a cellulose aerogel (CA) network named as HFO@CA	474:521	Herein, a novel nanocomposite was proposed for phosphate removal by confining hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA.					
36371568	2	9	with	adsorbents	287:296	arg1	capacity					319:326	high adsorption capacity	303:326	high adsorption capacity	303:326	Despite many efforts devoted to the adsorptive removal of phosphate from water, the development of new adsorbents with high adsorption capacity is highly desirable.					
36371568	6	10	theme	HFO	869:871	arg1	CA					873:874	HFO@CA	869:874	HFO@CA	869:874	The maximum adsorption capacity of HFO@CA occurs at near-acidic pH. With increasing temperature, the composite adsorbent is more favorable for phosphate adsorption.					
36371568	8	11	theme	@	1151:1151	arg1	CA					1152:1153	HFO@CA	1148:1153	HFO@CA	1148:1153	HFO@CA shows excellent adsorption selectivity in solutions containing competitive anions at higher levels.					
36371568	9	12	contain	has	1372:1374	arg1	CA					1369:1370	HFO@CA	1365:1370	HFO@CA	1365:1370	In addition, five cycles of the phosphate adsorption experiments without obvious capacity loss indicated that HFO@CA has great regenerability.					
36371568	9	12	contain	has	1372:1374	arg2	regenerability					1382:1395	great regenerability	1376:1395	great regenerability	1376:1395	In addition, five cycles of the phosphate adsorption experiments without obvious capacity loss indicated that HFO@CA has great regenerability.					
36371568	3	13	theme	HFO	516:518	arg1	CA					520:521	HFO@CA	516:521	HFO@CA	516:521	Herein, a novel nanocomposite was proposed for phosphate removal by confining hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA.					
36371568	4	14	theme	HFO	709:711	arg1	nanoparticles					713:725	the HFO nanoparticles	705:725	the HFO nanoparticles	705:725	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	3	15	theme	@	519:519	arg1	CA					520:521	HFO@CA	516:521	HFO@CA	516:521	Herein, a novel nanocomposite was proposed for phosphate removal by confining hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA.					
36371568	8	16	theme	HFO	1148:1150	arg1	CA					1152:1153	HFO@CA	1148:1153	HFO@CA	1148:1153	HFO@CA shows excellent adsorption selectivity in solutions containing competitive anions at higher levels.					
36371568	10	17	theme	wide	1442:1445	arg1	field					1447:1451	a wide field	1440:1451	a wide field of application	1440:1466	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	8	18	theme	higher	1240:1245	arg1	levels					1247:1252	higher levels	1240:1252	higher levels	1240:1252	HFO@CA shows excellent adsorption selectivity in solutions containing competitive anions at higher levels.					
36371568	10	19	theme	HFO	1429:1431	arg1	CA					1433:1434	HFO@CA	1429:1434	HFO@CA	1429:1434	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	9	20	theme	phosphate	1287:1295	arg1	experiments					1308:1318	the phosphate adsorption experiments	1283:1318	the phosphate adsorption experiments without obvious capacity loss	1283:1348	In addition, five cycles of the phosphate adsorption experiments without obvious capacity loss indicated that HFO@CA has great regenerability.					
36371568	3	21	theme	ferric	436:441	arg1	HFO					450:452	HFO	450:452	HFO	450:452	Herein, a novel nanocomposite was proposed for phosphate removal by confining hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA.					
36371568	3	21	theme	ferric	436:441	arg1	oxide					443:447	hydrated ferric oxide	427:447	hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA	427:521	Herein, a novel nanocomposite was proposed for phosphate removal by confining hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA.					
36371568	6	22	theme	adsorption	846:855	arg1	capacity					857:864	The maximum adsorption capacity	834:864	The maximum adsorption capacity of HFO@CA	834:874	The maximum adsorption capacity of HFO@CA occurs at near-acidic pH. With increasing temperature, the composite adsorbent is more favorable for phosphate adsorption.					
36371568	7	23	theme	phosphate	1064:1072	arg1	removal					1074:1080	phosphate removal	1064:1080	phosphate removal	1064:1080	Moreover, the hybrid aerogel exhibited fast kinetic behavior for phosphate removal, which could be accurately depicted by pseudo-second-order model.					
36371568	6	24	theme	phosphate	977:985	arg1	adsorption					987:996	phosphate adsorption	977:996	phosphate adsorption	977:996	The maximum adsorption capacity of HFO@CA occurs at near-acidic pH. With increasing temperature, the composite adsorbent is more favorable for phosphate adsorption.					
36371568	3	25	theme	oxide	443:447	arg1	nanoparticles					455:467	hydrated ferric oxide (HFO) nanoparticles	427:467	hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA	427:521	Herein, a novel nanocomposite was proposed for phosphate removal by confining hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA.					
36371568	6	26	theme	maximum	838:844	arg1	capacity					857:864	The maximum adsorption capacity	834:864	The maximum adsorption capacity of HFO@CA	834:874	The maximum adsorption capacity of HFO@CA occurs at near-acidic pH. With increasing temperature, the composite adsorbent is more favorable for phosphate adsorption.					
36371568	7	27	theme	kinetic	1043:1049	arg1	behavior					1051:1058	fast kinetic behavior	1038:1058	fast kinetic behavior	1038:1058	Moreover, the hybrid aerogel exhibited fast kinetic behavior for phosphate removal, which could be accurately depicted by pseudo-second-order model.					
36371568	2	28	theme	adsorption	308:317	arg1	capacity					319:326	high adsorption capacity	303:326	high adsorption capacity	303:326	Despite many efforts devoted to the adsorptive removal of phosphate from water, the development of new adsorbents with high adsorption capacity is highly desirable.					
36371568	10	29	theme	renewable	1616:1624	arg1	resources					1636:1644	renewable cellulose resources	1616:1644	renewable cellulose resources	1616:1644	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	0	30	theme	Renewable	0:8	arg1	cellulose					10:18	Renewable cellulose	0:18	Renewable cellulose	0:18	Renewable cellulose aerogel embedded with nano-HFO for preferable phosphate capture from aqueous solution.					
36371568	9	31	theme	experiments	1308:1318	arg1	cycles					1273:1278	five cycles	1268:1278	five cycles of the phosphate adsorption experiments without obvious capacity loss	1268:1348	In addition, five cycles of the phosphate adsorption experiments without obvious capacity loss indicated that HFO@CA has great regenerability.					
36371568	2	32	theme	many	192:195	arg1	efforts					197:203	many efforts	192:203	many efforts devoted to the adsorptive removal of phosphate from water	192:261	Despite many efforts devoted to the adsorptive removal of phosphate from water, the development of new adsorbents with high adsorption capacity is highly desirable.					
36371568	2	33	theme	high	303:306	arg1	capacity					319:326	high adsorption capacity	303:326	high adsorption capacity	303:326	Despite many efforts devoted to the adsorptive removal of phosphate from water, the development of new adsorbents with high adsorption capacity is highly desirable.					
36371568	4	34	theme	nanoparticles	713:725	arg1	utilization					690:700	the utilization	686:700	the utilization of the HFO nanoparticles	686:725	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	3	35	theme	hydrated	427:434	arg1	HFO					450:452	HFO	450:452	HFO	450:452	Herein, a novel nanocomposite was proposed for phosphate removal by confining hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA.					
36371568	3	35	theme	hydrated	427:434	arg1	oxide					443:447	hydrated ferric oxide	427:447	hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA	427:521	Herein, a novel nanocomposite was proposed for phosphate removal by confining hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA.					
36371568	3	36	theme	novel	359:363	arg1	nanocomposite					365:377	a novel nanocomposite	357:377	a novel nanocomposite	357:377	Herein, a novel nanocomposite was proposed for phosphate removal by confining hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA.					
36371568	8	37	theme	excellent	1161:1169	arg1	selectivity					1182:1192	excellent adsorption selectivity	1161:1192	excellent adsorption selectivity in solutions containing competitive anions at higher levels	1161:1252	HFO@CA shows excellent adsorption selectivity in solutions containing competitive anions at higher levels.					
36371568	1	38	theme	Excess	107:112	arg1	phosphate					114:122	Excess phosphate	107:122	Excess phosphate in water	107:131	Excess phosphate in water can cause eutrophication, which must be addressed.					
36371568	5	39	theme	phosphate	790:798	arg1	uptake					800:805	the phosphate uptake	786:805	the phosphate uptake	786:805	Batch adsorption experiments were carried out to evaluate the phosphate uptake by the prepared adsorbent.					
36371568	9	40	theme	@	1368:1368	arg1	CA					1369:1370	HFO@CA	1365:1370	HFO@CA	1365:1370	In addition, five cycles of the phosphate adsorption experiments without obvious capacity loss indicated that HFO@CA has great regenerability.					
36371568	9	41	theme	obvious	1328:1334	arg1	loss					1345:1348	obvious capacity loss	1328:1348	obvious capacity loss	1328:1348	In addition, five cycles of the phosphate adsorption experiments without obvious capacity loss indicated that HFO@CA has great regenerability.					
36371568	7	42	theme	fast	1038:1041	arg1	behavior					1051:1058	fast kinetic behavior	1038:1058	fast kinetic behavior	1038:1058	Moreover, the hybrid aerogel exhibited fast kinetic behavior for phosphate removal, which could be accurately depicted by pseudo-second-order model.					
36371568	2	43	theme	adsorbents	287:296	arg1	desirable					338:346	desirable	338:346	desirable	338:346	Despite many efforts devoted to the adsorptive removal of phosphate from water, the development of new adsorbents with high adsorption capacity is highly desirable.					
36371568	2	43	theme	adsorbents	287:296	arg1	development					268:278	the development	264:278	the development of new adsorbents with high adsorption capacity	264:326	Despite many efforts devoted to the adsorptive removal of phosphate from water, the development of new adsorbents with high adsorption capacity is highly desirable.					
36371568	4	44	theme	structure	590:598	arg1	characteristics					544:558	the characteristics	540:558	the characteristics of the low density and porous structure of CA	540:604	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	5	45	theme	adsorption	734:743	arg1	experiments					745:755	Batch adsorption experiments	728:755	Batch adsorption experiments	728:755	Batch adsorption experiments were carried out to evaluate the phosphate uptake by the prepared adsorbent.					
36371568	9	46	theme	capacity	1336:1343	arg1	loss					1345:1348	obvious capacity loss	1328:1348	obvious capacity loss	1328:1348	In addition, five cycles of the phosphate adsorption experiments without obvious capacity loss indicated that HFO@CA has great regenerability.					
36371568	7	47	theme	hybrid	1013:1018	arg1	aerogel					1020:1026	the hybrid aerogel	1009:1026	the hybrid aerogel	1009:1026	Moreover, the hybrid aerogel exhibited fast kinetic behavior for phosphate removal, which could be accurately depicted by pseudo-second-order model.					
36371568	10	48	theme	new	1548:1550	arg1	strategy					1552:1559	a new strategy	1546:1559	a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources	1546:1644	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	2	49	from	water	257:261	arg1	removal					231:237	the adsorptive removal	216:237	the adsorptive removal of phosphate from water	216:261	Despite many efforts devoted to the adsorptive removal of phosphate from water, the development of new adsorbents with high adsorption capacity is highly desirable.					
36371568	4	50	theme	internal	611:618	arg1	surface					620:626	the internal surface	607:626	the internal surface of the nanocomposite	607:647	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	4	50	theme	internal	611:618	arg1	accessible					657:666	accessible	657:666	accessible	657:666	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	8	51	theme	adsorption	1171:1180	arg1	selectivity					1182:1192	excellent adsorption selectivity	1161:1192	excellent adsorption selectivity in solutions containing competitive anions at higher levels	1161:1252	HFO@CA shows excellent adsorption selectivity in solutions containing competitive anions at higher levels.					
36371568	7	52	theme	pseudo-second-order	1121:1139	arg1	model					1141:1145	pseudo-second-order model	1121:1145	pseudo-second-order model	1121:1145	Moreover, the hybrid aerogel exhibited fast kinetic behavior for phosphate removal, which could be accurately depicted by pseudo-second-order model.					
36371568	0	53	with	embedded	28:35	arg1	nano-HFO					42:49	nano-HFO	42:49	nano-HFO for preferable phosphate capture from aqueous solution	42:104	Renewable cellulose aerogel embedded with nano-HFO for preferable phosphate capture from aqueous solution.					
36371568	4	54	theme	CA	603:604	arg1	density					571:577	low density	567:577	low density	567:577	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	4	54	theme	CA	603:604	arg1	structure					590:598	porous structure	583:598	porous structure	583:598	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	0	55	theme	preferable	55:64	arg1	capture					76:82	preferable phosphate capture	55:82	preferable phosphate capture from aqueous solution	55:104	Renewable cellulose aerogel embedded with nano-HFO for preferable phosphate capture from aqueous solution.					
36371568	10	56	theme	phosphate	1491:1499	arg1	removal					1501:1507	phosphate removal	1491:1507	phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources	1491:1644	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	10	57	from	wastewater	1514:1523	arg1	prospects					1478:1486	good prospects	1473:1486	good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources	1473:1644	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	10	57	from	wastewater	1514:1523	arg1	removal					1501:1507	phosphate removal	1491:1507	phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources	1491:1644	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	2	58	theme	adsorptive	220:229	arg1	removal					231:237	the adsorptive removal	216:237	the adsorptive removal of phosphate from water	216:261	Despite many efforts devoted to the adsorptive removal of phosphate from water, the development of new adsorbents with high adsorption capacity is highly desirable.					
36371568	8	59	from	selectivity	1182:1192	arg1	solutions					1197:1205	solutions	1197:1205	solutions containing competitive anions at higher levels	1197:1252	HFO@CA shows excellent adsorption selectivity in solutions containing competitive anions at higher levels.					
36371568	3	60	theme	cellulose	476:484	arg1	network					499:505	a cellulose aerogel (CA) network	474:505	a cellulose aerogel (CA) network named as HFO@CA	474:521	Herein, a novel nanocomposite was proposed for phosphate removal by confining hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA.					
36371568	3	61	theme	phosphate	396:404	arg1	removal					406:412	phosphate removal	396:412	phosphate removal	396:412	Herein, a novel nanocomposite was proposed for phosphate removal by confining hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA.					
36371568	3	62	theme	aerogel	486:492	arg1	network					499:505	a cellulose aerogel (CA) network	474:505	a cellulose aerogel (CA) network named as HFO@CA	474:521	Herein, a novel nanocomposite was proposed for phosphate removal by confining hydrated ferric oxide (HFO) nanoparticles into a cellulose aerogel (CA) network named as HFO@CA.					
36371568	9	63	theme	HFO	1365:1367	arg1	CA					1369:1370	HFO@CA	1365:1370	HFO@CA	1365:1370	In addition, five cycles of the phosphate adsorption experiments without obvious capacity loss indicated that HFO@CA has great regenerability.					
36371568	1	64	from	phosphate	114:122	arg1	water					127:131	water	127:131	water	127:131	Excess phosphate in water can cause eutrophication, which must be addressed.					
36371568	4	65	theme	density	571:577	arg1	characteristics					544:558	the characteristics	540:558	the characteristics of the low density and porous structure of CA	540:604	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	10	66	theme	good	1473:1476	arg1	prospects					1478:1486	good prospects	1473:1486	good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources	1473:1644	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	4	67	dep	density	571:577	arg1	the					563:565	the	563:565	the	563:565	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	2	68	theme	new	283:285	arg1	adsorbents					287:296	new adsorbents	283:296	new adsorbents with high adsorption capacity	283:326	Despite many efforts devoted to the adsorptive removal of phosphate from water, the development of new adsorbents with high adsorption capacity is highly desirable.					
36371568	10	69	contain	has	1436:1438	arg2	field					1447:1451	a wide field	1440:1451	a wide field of application	1440:1466	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	10	69	contain	has	1436:1438	arg1	CA					1433:1434	HFO@CA	1429:1434	HFO@CA	1429:1434	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	4	70	theme	low	567:569	arg1	density					571:577	low density	567:577	low density	567:577	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	10	71	theme	application	1456:1466	arg1	field					1447:1451	a wide field	1440:1451	a wide field of application	1440:1466	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	0	72	from	solution	97:104	arg1	capture					76:82	preferable phosphate capture	55:82	preferable phosphate capture from aqueous solution	55:104	Renewable cellulose aerogel embedded with nano-HFO for preferable phosphate capture from aqueous solution.					
36371568	4	73	theme	nanocomposite	635:647	arg1	surface					620:626	the internal surface	607:626	the internal surface of the nanocomposite	607:647	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	4	73	theme	nanocomposite	635:647	arg1	accessible					657:666	accessible	657:666	accessible	657:666	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	10	74	theme	cellulose	1626:1634	arg1	resources					1636:1644	renewable cellulose resources	1616:1644	renewable cellulose resources	1616:1644	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	4	75	theme	porous	583:588	arg1	structure					590:598	porous structure	583:598	porous structure	583:598	Benefiting from the characteristics of the low density and porous structure of CA, the internal surface of the nanocomposite is more accessible and thus improves the utilization of the HFO nanoparticles.					
36371568	0	76	theme	phosphate	66:74	arg1	capture					76:82	preferable phosphate capture	55:82	preferable phosphate capture from aqueous solution	55:104	Renewable cellulose aerogel embedded with nano-HFO for preferable phosphate capture from aqueous solution.					
36371568	2	77	theme	phosphate	242:250	arg1	removal					231:237	the adsorptive removal	216:237	the adsorptive removal of phosphate from water	216:261	Despite many efforts devoted to the adsorptive removal of phosphate from water, the development of new adsorbents with high adsorption capacity is highly desirable.					
36371568	8	78	theme	competitive	1218:1228	arg1	anions					1230:1235	competitive anions	1218:1235	competitive anions	1218:1235	HFO@CA shows excellent adsorption selectivity in solutions containing competitive anions at higher levels.					
36371568	10	79	theme	excellent	1588:1596	arg1	performance					1598:1608	excellent performance	1588:1608	excellent performance using renewable cellulose resources	1588:1644	These results demonstrate that HFO@CA has a wide field of application with good prospects in phosphate removal from wastewater, which also provides a new strategy to prepare adsorbents with excellent performance using renewable cellulose resources.					
36371568	9	80	theme	great	1376:1380	arg1	regenerability					1382:1395	great regenerability	1376:1395	great regenerability	1376:1395	In addition, five cycles of the phosphate adsorption experiments without obvious capacity loss indicated that HFO@CA has great regenerability.					
36345885	2	0	theme	diacrylate/cellulose	434:453	arg1	aerogel					466:472	a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel	396:472	a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel	396:472	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	5	1	theme	polymerase	1051:1060	arg1	reaction					1068:1075	real-time polymerase chain reaction	1041:1075	real-time polymerase chain reaction	1041:1075	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	4	2	theme	geometry	782:789	arg1	parameters					791:800	geometry parameters	782:800	geometry parameters	782:800	Then, nine groups of scaffolds with different PRs ranging from -0.5 to 0.85 are designed by adjusting geometry parameters and fabricated by using stereolithography and freeze-drying techniques.					
36345885	2	3	theme	reentrant	509:517	arg1	models					538:543	the hexagonal, reentrant, and semireentrant models	494:543	the hexagonal, reentrant, and semireentrant models	494:543	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	0	4	theme	mesenchymal	142:152	arg1	cell					159:162	bone marrow mesenchymal stem cell	130:162	bone marrow mesenchymal stem cell	130:162	Tuning the Poisson's ratio of poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel scaffold precisely for cultivation of bone marrow mesenchymal stem cell.					
36345885	5	5	theme	chain	1062:1066	arg1	reaction					1068:1075	real-time polymerase chain reaction	1041:1075	real-time polymerase chain reaction	1041:1075	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	5	6	theme	fluorescence	1000:1011	arg1	observation					1024:1034	fluorescence microscope observation	1000:1034	fluorescence microscope observation	1000:1034	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	6	7	theme	various	1235:1241	arg1	environments					1250:1261	various stress environments	1235:1261	various stress environments	1235:1261	The results reflect that the scaffolds with different PR can provide various stress environments for cells, and the scaffold with zero PR is the most suitable for BMSc differentiating into chondrocytes during early culture experiments.					
36345885	0	8	theme	aerogel	84:90	arg1	scaffold					92:99	nanofibril aerogel scaffold	73:99	nanofibril aerogel scaffold	73:99	Tuning the Poisson's ratio of poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel scaffold precisely for cultivation of bone marrow mesenchymal stem cell.					
36345885	5	9	theme	reaction	1068:1075	arg1	experiments					1077:1087	assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments	993:1087	assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments	993:1087	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	5	10	theme	mesenchymal	900:910	arg1	BMSc					924:927	BMSc	924:927	BMSc	924:927	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	5	10	theme	mesenchymal	900:910	arg1	cells					917:921	bone marrow mesenchymal stem cells	888:921	bone marrow mesenchymal stem cells (BMSc)	888:928	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	5	11	theme	BMSc	1160:1163	arg1	differentiation					1141:1155	differentiation	1141:1155	differentiation	1141:1155	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	5	11	theme	BMSc	1160:1163	arg1	proliferation					1123:1135	proliferation	1123:1135	proliferation	1123:1135	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	0	12	theme	nanofibril	73:82	arg1	scaffold					92:99	nanofibril aerogel scaffold	73:99	nanofibril aerogel scaffold	73:99	Tuning the Poisson's ratio of poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel scaffold precisely for cultivation of bone marrow mesenchymal stem cell.					
36345885	5	13	theme	microscope	1013:1022	arg1	observation					1024:1034	fluorescence microscope observation	1000:1034	fluorescence microscope observation	1000:1034	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	5	14	theme	stem	912:915	arg1	BMSc					924:927	BMSc	924:927	BMSc	924:927	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	5	14	theme	stem	912:915	arg1	cells					917:921	bone marrow mesenchymal stem cells	888:921	bone marrow mesenchymal stem cells (BMSc)	888:928	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	4	15	with	scaffolds	701:709	arg1	PRs					726:728	different PRs	716:728	different PRs ranging from -0.5 to 0.85	716:754	Then, nine groups of scaffolds with different PRs ranging from -0.5 to 0.85 are designed by adjusting geometry parameters and fabricated by using stereolithography and freeze-drying techniques.					
36345885	5	16	theme	observation	1024:1034	arg1	experiments					1077:1087	assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments	993:1087	assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments	993:1087	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	6	17	theme	different	1210:1218	arg1	PR					1220:1221	different PR	1210:1221	different PR	1210:1221	The results reflect that the scaffolds with different PR can provide various stress environments for cells, and the scaffold with zero PR is the most suitable for BMSc differentiating into chondrocytes during early culture experiments.					
36345885	2	18	theme	cellular	354:361	arg1	scaffolds					374:382	cellular structured scaffolds	354:382	cellular structured scaffolds	354:382	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	2	19	theme	poly	412:415	arg1	aerogel					466:472	a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel	396:472	a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel	396:472	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	2	20	theme	new	398:400	arg1	aerogel					466:472	a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel	396:472	a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel	396:472	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	6	21	with	scaffold	1282:1289	arg1	PR					1301:1302	zero PR	1296:1302	zero PR	1296:1302	The results reflect that the scaffolds with different PR can provide various stress environments for cells, and the scaffold with zero PR is the most suitable for BMSc differentiating into chondrocytes during early culture experiments.					
36345885	1	22	theme	Tissue	165:170	arg1	engineering					172:182	Tissue engineering	165:182	Tissue engineering (TE) scaffolds with appropriate Poisson's ratio (PR)	165:235	Tissue engineering (TE) scaffolds with appropriate Poisson's ratio (PR) are suitable for mimicking the environment of native tissues on which cells could survive and thrive better.					
36345885	1	22	theme	Tissue	165:170	arg1	TE					185:186	TE	185:186	TE	185:186	Tissue engineering (TE) scaffolds with appropriate Poisson's ratio (PR) are suitable for mimicking the environment of native tissues on which cells could survive and thrive better.					
36345885	2	23	theme	composite	402:410	arg1	poly					412:415	composite poly	402:415	a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel	396:472	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	2	23	theme	composite	402:410	arg1	glycol					426:431	ethylene glycol	417:431	ethylene glycol	417:431	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	3	24	theme	precise	648:654	arg1	regulation					656:665	precise regulation	648:665	precise regulation of their PR	648:677	Scaffolds with different geometry parameters (l, t, α) are designed and simulated by COMSOL to enable precise regulation of their PR.					
36345885	6	25	theme	stress	1243:1248	arg1	environments					1250:1261	various stress environments	1235:1261	various stress environments	1235:1261	The results reflect that the scaffolds with different PR can provide various stress environments for cells, and the scaffold with zero PR is the most suitable for BMSc differentiating into chondrocytes during early culture experiments.					
36345885	2	26	theme	semireentrant	524:536	arg1	models					538:543	the hexagonal, reentrant, and semireentrant models	494:543	the hexagonal, reentrant, and semireentrant models	494:543	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	1	27	theme	engineering	172:182	arg1	scaffolds					189:197	Tissue engineering (TE) scaffolds	165:197	Tissue engineering (TE) scaffolds with appropriate Poisson's ratio (PR)	165:235	Tissue engineering (TE) scaffolds with appropriate Poisson's ratio (PR) are suitable for mimicking the environment of native tissues on which cells could survive and thrive better.					
36345885	0	28	theme	Tuning	0:5	arg1	ratio					21:25	Tuning the Poisson's ratio	0:25	Tuning the Poisson's ratio of poly(ethylene glycol)	0:50	Tuning the Poisson's ratio of poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel scaffold precisely for cultivation of bone marrow mesenchymal stem cell.					
36345885	2	29	theme	hexagonal	498:506	arg1	models					538:543	the hexagonal, reentrant, and semireentrant models	494:543	the hexagonal, reentrant, and semireentrant models	494:543	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	1	30	with	scaffolds	189:197	arg1	PR					233:234	PR	233:234	PR	233:234	Tissue engineering (TE) scaffolds with appropriate Poisson's ratio (PR) are suitable for mimicking the environment of native tissues on which cells could survive and thrive better.					
36345885	1	30	with	scaffolds	189:197	arg1	ratio					226:230	appropriate Poisson's ratio	204:230	appropriate Poisson's ratio (PR)	204:235	Tissue engineering (TE) scaffolds with appropriate Poisson's ratio (PR) are suitable for mimicking the environment of native tissues on which cells could survive and thrive better.					
36345885	7	31	theme	cells-suitable	1500:1513	arg1	environment					1515:1525	a cells-suitable environment	1498:1525	a cells-suitable environment for scaffold fabrication for TE	1498:1557	This study suggests that tuning PR precisely is an attractive and effective strategy to provide a cells-suitable environment for scaffold fabrication for TE.					
36345885	5	32	theme	bone	888:891	arg1	marrow					893:898	bone marrow	888:898	bone marrow mesenchymal stem cells (BMSc)	888:928	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	5	33	dep	proliferation	1123:1135	arg1	the					1119:1121	the	1119:1121	the	1119:1121	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	4	34	dep	0.85	751:754	arg1	to					748:749	to	748:749	to	748:749	Then, nine groups of scaffolds with different PRs ranging from -0.5 to 0.85 are designed by adjusting geometry parameters and fabricated by using stereolithography and freeze-drying techniques.					
36345885	6	35	theme	early	1375:1379	arg1	experiments					1389:1399	early culture experiments	1375:1399	early culture experiments	1375:1399	The results reflect that the scaffolds with different PR can provide various stress environments for cells, and the scaffold with zero PR is the most suitable for BMSc differentiating into chondrocytes during early culture experiments.					
36345885	5	36	theme	marrow	893:898	arg1	BMSc					924:927	BMSc	924:927	BMSc	924:927	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	5	36	theme	marrow	893:898	arg1	cells					917:921	bone marrow mesenchymal stem cells	888:921	bone marrow mesenchymal stem cells (BMSc)	888:928	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	3	37	dep	parameters	580:589	arg1	α					598:598	α	598:598	α	598:598	Scaffolds with different geometry parameters (l, t, α) are designed and simulated by COMSOL to enable precise regulation of their PR.					
36345885	3	37	dep	parameters	580:589	arg1	t					595:595	t	595:595	t	595:595	Scaffolds with different geometry parameters (l, t, α) are designed and simulated by COMSOL to enable precise regulation of their PR.					
36345885	3	37	dep	parameters	580:589	arg1	l					592:592	l	592:592	l	592:592	Scaffolds with different geometry parameters (l, t, α) are designed and simulated by COMSOL to enable precise regulation of their PR.					
36345885	3	38	theme	PR	676:677	arg1	regulation					656:665	precise regulation	648:665	precise regulation of their PR	648:677	Scaffolds with different geometry parameters (l, t, α) are designed and simulated by COMSOL to enable precise regulation of their PR.					
36345885	0	39	theme	bone	130:133	arg1	marrow					135:140	bone marrow	130:140	bone marrow mesenchymal stem cell	130:162	Tuning the Poisson's ratio of poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel scaffold precisely for cultivation of bone marrow mesenchymal stem cell.					
36345885	5	40	theme	assay	993:997	arg1	experiments					1077:1087	assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments	993:1087	assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments	993:1087	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	2	41	theme	ethylene	417:424	arg1	poly					412:415	composite poly	402:415	a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel	396:472	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	2	41	theme	ethylene	417:424	arg1	glycol					426:431	ethylene glycol	417:431	ethylene glycol	417:431	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	7	42	theme	effective	1468:1476	arg1	strategy					1478:1485	an attractive and effective strategy	1450:1485	an attractive and effective strategy to provide a cells-suitable environment for scaffold fabrication for TE	1450:1557	This study suggests that tuning PR precisely is an attractive and effective strategy to provide a cells-suitable environment for scaffold fabrication for TE.					
36345885	7	42	theme	effective	1468:1476	arg1	PR					1434:1435	tuning PR	1427:1435	tuning PR	1427:1435	This study suggests that tuning PR precisely is an attractive and effective strategy to provide a cells-suitable environment for scaffold fabrication for TE.					
36345885	3	43	theme	different	561:569	arg1	parameters					580:589	different geometry parameters	561:589	different geometry parameters (l, t, α)	561:599	Scaffolds with different geometry parameters (l, t, α) are designed and simulated by COMSOL to enable precise regulation of their PR.					
36345885	2	44	theme	structured	363:372	arg1	scaffolds					374:382	cellular structured scaffolds	354:382	cellular structured scaffolds	354:382	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	2	45	theme	models	538:543	arg1	prototypes					480:489	prototypes	480:489	prototypes of the hexagonal, reentrant, and semireentrant models	480:543	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	0	46	theme	ethylene	35:42	arg1	poly					30:33	poly	30:33	poly(ethylene glycol)	30:50	Tuning the Poisson's ratio of poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel scaffold precisely for cultivation of bone marrow mesenchymal stem cell.					
36345885	0	46	theme	ethylene	35:42	arg1	glycol					44:49	ethylene glycol	35:49	ethylene glycol	35:49	Tuning the Poisson's ratio of poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel scaffold precisely for cultivation of bone marrow mesenchymal stem cell.					
36345885	4	47	theme	stereolithography	826:842	arg1	techniques					862:871	stereolithography and freeze-drying techniques	826:871	stereolithography and freeze-drying techniques	826:871	Then, nine groups of scaffolds with different PRs ranging from -0.5 to 0.85 are designed by adjusting geometry parameters and fabricated by using stereolithography and freeze-drying techniques.					
36345885	6	48	theme	culture	1381:1387	arg1	experiments					1389:1399	early culture experiments	1375:1399	early culture experiments	1375:1399	The results reflect that the scaffolds with different PR can provide various stress environments for cells, and the scaffold with zero PR is the most suitable for BMSc differentiating into chondrocytes during early culture experiments.					
36345885	0	49	theme	poly	30:33	arg1	ratio					21:25	Tuning the Poisson's ratio	0:25	Tuning the Poisson's ratio of poly(ethylene glycol)	0:50	Tuning the Poisson's ratio of poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel scaffold precisely for cultivation of bone marrow mesenchymal stem cell.					
36345885	1	50	theme	native	283:288	arg1	tissues					290:296	native tissues	283:296	native tissues on which cells could survive and thrive better	283:343	Tissue engineering (TE) scaffolds with appropriate Poisson's ratio (PR) are suitable for mimicking the environment of native tissues on which cells could survive and thrive better.					
36345885	0	51	theme	marrow	135:140	arg1	cell					159:162	bone marrow mesenchymal stem cell	130:162	bone marrow mesenchymal stem cell	130:162	Tuning the Poisson's ratio of poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel scaffold precisely for cultivation of bone marrow mesenchymal stem cell.					
36345885	7	52	theme	attractive	1453:1462	arg1	strategy					1478:1485	an attractive and effective strategy	1450:1485	an attractive and effective strategy to provide a cells-suitable environment for scaffold fabrication for TE	1450:1557	This study suggests that tuning PR precisely is an attractive and effective strategy to provide a cells-suitable environment for scaffold fabrication for TE.					
36345885	7	52	theme	attractive	1453:1462	arg1	PR					1434:1435	tuning PR	1427:1435	tuning PR	1427:1435	This study suggests that tuning PR precisely is an attractive and effective strategy to provide a cells-suitable environment for scaffold fabrication for TE.					
36345885	1	53	theme	tissues	290:296	arg1	environment					268:278	the environment	264:278	the environment of native tissues on which cells could survive and thrive better	264:343	Tissue engineering (TE) scaffolds with appropriate Poisson's ratio (PR) are suitable for mimicking the environment of native tissues on which cells could survive and thrive better.					
36345885	0	54	theme	cell	159:162	arg1	cultivation					115:125	cultivation	115:125	cultivation of bone marrow mesenchymal stem cell	115:162	Tuning the Poisson's ratio of poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel scaffold precisely for cultivation of bone marrow mesenchymal stem cell.					
36345885	7	55	theme	tuning	1427:1432	arg1	strategy					1478:1485	an attractive and effective strategy	1450:1485	an attractive and effective strategy to provide a cells-suitable environment for scaffold fabrication for TE	1450:1557	This study suggests that tuning PR precisely is an attractive and effective strategy to provide a cells-suitable environment for scaffold fabrication for TE.					
36345885	7	55	theme	tuning	1427:1432	arg1	PR					1434:1435	tuning PR	1427:1435	tuning PR	1427:1435	This study suggests that tuning PR precisely is an attractive and effective strategy to provide a cells-suitable environment for scaffold fabrication for TE.					
36345885	1	56	theme	appropriate	204:214	arg1	PR					233:234	PR	233:234	PR	233:234	Tissue engineering (TE) scaffolds with appropriate Poisson's ratio (PR) are suitable for mimicking the environment of native tissues on which cells could survive and thrive better.					
36345885	1	56	theme	appropriate	204:214	arg1	ratio					226:230	appropriate Poisson's ratio	204:230	appropriate Poisson's ratio (PR)	204:235	Tissue engineering (TE) scaffolds with appropriate Poisson's ratio (PR) are suitable for mimicking the environment of native tissues on which cells could survive and thrive better.					
36345885	0	57	theme	stem	154:157	arg1	cell					159:162	bone marrow mesenchymal stem cell	130:162	bone marrow mesenchymal stem cell	130:162	Tuning the Poisson's ratio of poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel scaffold precisely for cultivation of bone marrow mesenchymal stem cell.					
36345885	4	58	theme	scaffolds	701:709	arg1	scaffolds					701:709	scaffolds	701:709	scaffolds with different PRs ranging from -0.5 to 0.85	701:754	Then, nine groups of scaffolds with different PRs ranging from -0.5 to 0.85 are designed by adjusting geometry parameters and fabricated by using stereolithography and freeze-drying techniques.					
36345885	4	58	theme	scaffolds	701:709	arg1	groups					691:696	nine groups	686:696	nine groups of scaffolds with different PRs ranging from -0.5 to 0.85	686:754	Then, nine groups of scaffolds with different PRs ranging from -0.5 to 0.85 are designed by adjusting geometry parameters and fabricated by using stereolithography and freeze-drying techniques.					
36345885	4	59	theme	freeze-drying	848:860	arg1	techniques					862:871	stereolithography and freeze-drying techniques	826:871	stereolithography and freeze-drying techniques	826:871	Then, nine groups of scaffolds with different PRs ranging from -0.5 to 0.85 are designed by adjusting geometry parameters and fabricated by using stereolithography and freeze-drying techniques.					
36345885	3	60	theme	geometry	571:578	arg1	parameters					580:589	different geometry parameters	561:589	different geometry parameters (l, t, α)	561:599	Scaffolds with different geometry parameters (l, t, α) are designed and simulated by COMSOL to enable precise regulation of their PR.					
36345885	7	61	theme	scaffold	1531:1538	arg1	fabrication					1540:1550	scaffold fabrication	1531:1550	scaffold fabrication for TE	1531:1557	This study suggests that tuning PR precisely is an attractive and effective strategy to provide a cells-suitable environment for scaffold fabrication for TE.					
36345885	2	62	theme	nanofibril	455:464	arg1	aerogel					466:472	a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel	396:472	a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel	396:472	Herein, cellular structured scaffolds are made by a new composite poly(ethylene glycol) diacrylate/cellulose nanofibril aerogel, with prototypes of the hexagonal, reentrant, and semireentrant models.					
36345885	5	63	theme	real-time	1041:1049	arg1	reaction					1068:1075	real-time polymerase chain reaction	1041:1075	real-time polymerase chain reaction	1041:1075	Subsequently, bone marrow mesenchymal stem cells (BMSc) are cultured on these scaffolds for 21 days, during which CCK8 assay, fluorescence microscope observation, and real-time polymerase chain reaction experiments are performed to characterize the proliferation and differentiation of BMSc.					
36345885	4	64	theme	different	716:724	arg1	PRs					726:728	different PRs	716:728	different PRs ranging from -0.5 to 0.85	716:754	Then, nine groups of scaffolds with different PRs ranging from -0.5 to 0.85 are designed by adjusting geometry parameters and fabricated by using stereolithography and freeze-drying techniques.					
36345885	6	65	with	scaffolds	1195:1203	arg1	PR					1220:1221	different PR	1210:1221	different PR	1210:1221	The results reflect that the scaffolds with different PR can provide various stress environments for cells, and the scaffold with zero PR is the most suitable for BMSc differentiating into chondrocytes during early culture experiments.					
36345885	3	66	with	Scaffolds	546:554	arg1	parameters					580:589	different geometry parameters	561:589	different geometry parameters (l, t, α)	561:599	Scaffolds with different geometry parameters (l, t, α) are designed and simulated by COMSOL to enable precise regulation of their PR.					
35526013	3	0	dep	develop	378:384	arg1	mixed					564:568	mixed	564:568	mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP)	564:629	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	1	1	theme	common	168:173	arg1	Osteoarthritis					143:156	Osteoarthritis	143:156	Osteoarthritis (OA)	143:161	Osteoarthritis (OA) is a common degenerative joint disease that can lead to disability.					
35526013	1	1	theme	common	168:173	arg1	disease					194:200	a common degenerative joint disease	166:200	a common degenerative joint disease that can lead to disability	166:228	Osteoarthritis (OA) is a common degenerative joint disease that can lead to disability.					
35526013	6	2	theme	@	951:951	arg1	gel					955:957	HPP@Cu gel	948:957	HPP@Cu gel	948:957	Our results indicate that HPP@Cu gel could efficiently remove RONS from inflammatory sites and promote repolarization of macrophages to an anti-inflammatory phenotype.					
35526013	1	3	theme	degenerative	175:186	arg1	Osteoarthritis					143:156	Osteoarthritis	143:156	Osteoarthritis (OA)	143:161	Osteoarthritis (OA) is a common degenerative joint disease that can lead to disability.					
35526013	1	3	theme	degenerative	175:186	arg1	disease					194:200	a common degenerative joint disease	166:200	a common degenerative joint disease that can lead to disability	166:228	Osteoarthritis (OA) is a common degenerative joint disease that can lead to disability.					
35526013	0	4	theme	scavenging	126:135	arg1	RONS					137:140	scavenging RONS	126:140	scavenging RONS	126:140	Multifunctional thermo-sensitive hydrogel for modulating the microenvironment in Osteoarthritis by polarizing macrophages and scavenging RONS.					
35526013	8	5	theme	inflammatory	1291:1302	arg1	diseases					1304:1311	inflammatory diseases	1291:1311	inflammatory diseases associated with oxidative stress	1291:1344	This study provides a reliable reference for the application of injectable hydrogels in inflammatory diseases associated with oxidative stress.					
35526013	4	6	theme	nitrogen	688:695	arg1	species					697:703	reactive oxygen and nitrogen species	668:703	species	697:703	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	4	7	theme	reactive	668:675	arg1	RONS					706:709	RONS	706:709	RONS	706:709	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	4	7	theme	reactive	668:675	arg1	oxygen					677:682	reactive oxygen and nitrogen species	668:703	oxygen	677:682	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	7	8	theme	Cu	1098:1099	arg1	therapy					1105:1111	The HPP@Cu gel therapy	1090:1111	The HPP@Cu gel therapy	1090:1111	The HPP@Cu gel therapy dramatically reduces cartilage degradation and inflammatory factor production in OA rats.					
35526013	4	9	theme	Cu	632:633	arg1	nano-scavenger					650:663	a novel nano-scavenger	642:663	a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity	642:758	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	4	9	theme	Cu	632:633	arg1	NDs					635:637	Cu NDs	632:637	Cu NDs	632:637	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	5	10	dep	in	828:829	arg1	situ					831:834	situ	831:834	situ	831:834	HPP@Cu gel is injected into the articular cavity, where it form an in situ gel that slowly released Cu NDs, HA, and PRP, prolonging the duration of drug action.					
35526013	0	11	from	microenvironment	61:76	arg1	Osteoarthritis					81:94	Osteoarthritis	81:94	Osteoarthritis	81:94	Multifunctional thermo-sensitive hydrogel for modulating the microenvironment in Osteoarthritis by polarizing macrophages and scavenging RONS.					
35526013	6	12	theme	HPP	948:950	arg1	gel					955:957	HPP@Cu gel	948:957	HPP@Cu gel	948:957	Our results indicate that HPP@Cu gel could efficiently remove RONS from inflammatory sites and promote repolarization of macrophages to an anti-inflammatory phenotype.					
35526013	2	13	theme	oxidative	296:304	arg1	stress					306:311	oxidative stress	296:311	oxidative stress	296:311	Blocking the complex malignant feedback loop system dominated by oxidative stress and pro-inflammatory factors is the key to treating OA.					
35526013	1	14	theme	joint	188:192	arg1	Osteoarthritis					143:156	Osteoarthritis	143:156	Osteoarthritis (OA)	143:161	Osteoarthritis (OA) is a common degenerative joint disease that can lead to disability.					
35526013	1	14	theme	joint	188:192	arg1	disease					194:200	a common degenerative joint disease	166:200	a common degenerative joint disease that can lead to disability	166:228	Osteoarthritis (OA) is a common degenerative joint disease that can lead to disability.					
35526013	3	15	theme	composite	404:412	arg1	gel					448:450	HPP@Cu gel	441:450	HPP@Cu gel	441:450	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	15	theme	composite	404:412	arg1	hydrogel					431:438	a multifunctional composite thermo-sensitive hydrogel	386:438	a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel)	386:451	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	15	theme	composite	404:412	arg1	matrix					540:545	the gel matrix	532:545	the gel matrix	532:545	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	0	16	theme	thermo-sensitive	16:31	arg1	hydrogel					33:40	Multifunctional thermo-sensitive hydrogel	0:40	Multifunctional thermo-sensitive hydrogel for modulating the microenvironment in Osteoarthritis by polarizing macrophages and scavenging RONS.	0:141	Multifunctional thermo-sensitive hydrogel for modulating the microenvironment in Osteoarthritis by polarizing macrophages and scavenging RONS.					
35526013	5	17	theme	in	828:829	arg1	gel					836:838	an in situ gel	825:838	an in situ gel that slowly released Cu NDs, HA, and PRP, prolonging the duration of drug action	825:919	HPP@Cu gel is injected into the articular cavity, where it form an in situ gel that slowly released Cu NDs, HA, and PRP, prolonging the duration of drug action.					
35526013	8	18	from	application	1252:1262	arg1	diseases					1304:1311	inflammatory diseases	1291:1311	inflammatory diseases associated with oxidative stress	1291:1344	This study provides a reliable reference for the application of injectable hydrogels in inflammatory diseases associated with oxidative stress.					
35526013	3	19	theme	@	444:444	arg1	gel					448:450	HPP@Cu gel	441:450	HPP@Cu gel	441:450	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	19	theme	@	444:444	arg1	hydrogel					431:438	a multifunctional composite thermo-sensitive hydrogel	386:438	a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel)	386:451	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	0	20	theme	Multifunctional	0:14	arg1	hydrogel					33:40	Multifunctional thermo-sensitive hydrogel	0:40	Multifunctional thermo-sensitive hydrogel for modulating the microenvironment in Osteoarthritis by polarizing macrophages and scavenging RONS.	0:141	Multifunctional thermo-sensitive hydrogel for modulating the microenvironment in Osteoarthritis by polarizing macrophages and scavenging RONS.					
35526013	5	21	theme	action	914:919	arg1	duration					897:904	the duration	893:904	the duration of drug action	893:919	HPP@Cu gel is injected into the articular cavity, where it form an in situ gel that slowly released Cu NDs, HA, and PRP, prolonging the duration of drug action.					
35526013	3	22	theme	gel	536:538	arg1	hydrogel					431:438	a multifunctional composite thermo-sensitive hydrogel	386:438	a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel)	386:451	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	22	theme	gel	536:538	arg1	matrix					540:545	the gel matrix	532:545	the gel matrix	532:545	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	23	theme	Cu	445:446	arg1	gel					448:450	HPP@Cu gel	441:450	HPP@Cu gel	441:450	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	23	theme	Cu	445:446	arg1	hydrogel					431:438	a multifunctional composite thermo-sensitive hydrogel	386:438	a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel)	386:451	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	4	24	theme	efficient	717:725	arg1	activity					751:758	efficient free radical scavenging activity	717:758	efficient free radical scavenging activity	717:758	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	7	25	from	degradation	1144:1154	arg1	rats					1197:1200	OA rats	1194:1200	OA rats	1194:1200	The HPP@Cu gel therapy dramatically reduces cartilage degradation and inflammatory factor production in OA rats.					
35526013	3	26	used	utilized	463:470	arg2	matrix					540:545	the gel matrix	532:545	the gel matrix	532:545	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	26	used	utilized	463:470	arg2	hydrogel					431:438	a multifunctional composite thermo-sensitive hydrogel	386:438	a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel)	386:451	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	26	used	utilized	463:470	arg2	gel					448:450	HPP@Cu gel	441:450	HPP@Cu gel	441:450	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	4	27	theme	radical	732:738	arg1	activity					751:758	efficient free radical scavenging activity	717:758	efficient free radical scavenging activity	717:758	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	7	28	theme	factor	1173:1178	arg1	production					1180:1189	inflammatory factor production	1160:1189	inflammatory factor production	1160:1189	The HPP@Cu gel therapy dramatically reduces cartilage degradation and inflammatory factor production in OA rats.					
35526013	6	29	theme	inflammatory	994:1005	arg1	sites					1007:1011	inflammatory sites	994:1011	inflammatory sites	994:1011	Our results indicate that HPP@Cu gel could efficiently remove RONS from inflammatory sites and promote repolarization of macrophages to an anti-inflammatory phenotype.					
35526013	3	30	theme	acid	511:514	arg1	mixture					521:527	hyaluronic acid (HA) mixture	500:527	hyaluronic acid (HA) mixture	500:527	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	8	31	theme	oxidative	1329:1337	arg1	stress					1339:1344	oxidative stress	1329:1344	oxidative stress	1329:1344	This study provides a reliable reference for the application of injectable hydrogels in inflammatory diseases associated with oxidative stress.					
35526013	2	32	theme	pro-inflammatory	317:332	arg1	factors					334:340	pro-inflammatory factors	317:340	pro-inflammatory factors	317:340	Blocking the complex malignant feedback loop system dominated by oxidative stress and pro-inflammatory factors is the key to treating OA.					
35526013	6	33	from	sites	1007:1011	arg1	RONS					984:987	RONS	984:987	RONS from inflammatory sites	984:1011	Our results indicate that HPP@Cu gel could efficiently remove RONS from inflammatory sites and promote repolarization of macrophages to an anti-inflammatory phenotype.					
35526013	5	34	theme	HPP	761:763	arg1	gel					768:770	HPP@Cu gel	761:770	HPP@Cu gel	761:770	HPP@Cu gel is injected into the articular cavity, where it form an in situ gel that slowly released Cu NDs, HA, and PRP, prolonging the duration of drug action.					
35526013	7	35	theme	inflammatory	1160:1171	arg1	production					1180:1189	inflammatory factor production	1160:1189	inflammatory factor production	1160:1189	The HPP@Cu gel therapy dramatically reduces cartilage degradation and inflammatory factor production in OA rats.					
35526013	7	36	theme	gel	1101:1103	arg1	therapy					1105:1111	The HPP@Cu gel therapy	1090:1111	The HPP@Cu gel therapy	1090:1111	The HPP@Cu gel therapy dramatically reduces cartilage degradation and inflammatory factor production in OA rats.					
35526013	2	37	theme	malignant	252:260	arg1	system					276:281	the complex malignant feedback loop system	240:281	the complex malignant feedback loop system dominated by oxidative stress and pro-inflammatory factors	240:340	Blocking the complex malignant feedback loop system dominated by oxidative stress and pro-inflammatory factors is the key to treating OA.					
35526013	7	38	theme	OA	1194:1195	arg1	rats					1197:1200	OA rats	1194:1200	OA rats	1194:1200	The HPP@Cu gel therapy dramatically reduces cartilage degradation and inflammatory factor production in OA rats.					
35526013	5	39	theme	@	764:764	arg1	gel					768:770	HPP@Cu gel	761:770	HPP@Cu gel	761:770	HPP@Cu gel is injected into the articular cavity, where it form an in situ gel that slowly released Cu NDs, HA, and PRP, prolonging the duration of drug action.					
35526013	3	40	theme	HPP	441:443	arg1	gel					448:450	HPP@Cu gel	441:450	HPP@Cu gel	441:450	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	40	theme	HPP	441:443	arg1	hydrogel					431:438	a multifunctional composite thermo-sensitive hydrogel	386:438	a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel)	386:451	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	4	41	with	oxygen	677:682	arg1	activity					751:758	efficient free radical scavenging activity	717:758	efficient free radical scavenging activity	717:758	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	2	42	theme	complex	244:250	arg1	system					276:281	the complex malignant feedback loop system	240:281	the complex malignant feedback loop system dominated by oxidative stress and pro-inflammatory factors	240:340	Blocking the complex malignant feedback loop system dominated by oxidative stress and pro-inflammatory factors is the key to treating OA.					
35526013	5	43	theme	Cu	765:766	arg1	gel					768:770	HPP@Cu gel	761:770	HPP@Cu gel	761:770	HPP@Cu gel is injected into the articular cavity, where it form an in situ gel that slowly released Cu NDs, HA, and PRP, prolonging the duration of drug action.					
35526013	3	44	theme	thermo-sensitive	414:429	arg1	gel					448:450	HPP@Cu gel	441:450	HPP@Cu gel	441:450	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	44	theme	thermo-sensitive	414:429	arg1	hydrogel					431:438	a multifunctional composite thermo-sensitive hydrogel	386:438	a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel)	386:451	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	44	theme	thermo-sensitive	414:429	arg1	matrix					540:545	the gel matrix	532:545	the gel matrix	532:545	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	45	theme	Cu	592:593	arg1	NDs					595:597	Cu NDs	592:597	Cu NDs	592:597	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	45	theme	Cu	592:593	arg1	nanodots					582:589	copper nanodots	575:589	copper nanodots (Cu NDs)	575:598	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	8	46	theme	hydrogels	1278:1286	arg1	application					1252:1262	the application	1248:1262	the application of injectable hydrogels in inflammatory diseases associated with oxidative stress	1248:1344	This study provides a reliable reference for the application of injectable hydrogels in inflammatory diseases associated with oxidative stress.					
35526013	6	47	theme	Cu	952:953	arg1	gel					955:957	HPP@Cu gel	948:957	HPP@Cu gel	948:957	Our results indicate that HPP@Cu gel could efficiently remove RONS from inflammatory sites and promote repolarization of macrophages to an anti-inflammatory phenotype.					
35526013	7	48	theme	cartilage	1134:1142	arg1	degradation					1144:1154	cartilage degradation	1134:1154	cartilage degradation	1134:1154	The HPP@Cu gel therapy dramatically reduces cartilage degradation and inflammatory factor production in OA rats.					
35526013	4	49	with	species	697:703	arg1	activity					751:758	efficient free radical scavenging activity	717:758	efficient free radical scavenging activity	717:758	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	4	50	theme	scavenging	740:749	arg1	activity					751:758	efficient free radical scavenging activity	717:758	efficient free radical scavenging activity	717:758	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	2	51	theme	treating	356:363	arg1	OA					365:366	treating OA	356:366	treating OA	356:366	Blocking the complex malignant feedback loop system dominated by oxidative stress and pro-inflammatory factors is the key to treating OA.					
35526013	6	52	theme	macrophages	1043:1053	arg1	repolarization					1025:1038	repolarization	1025:1038	repolarization of macrophages to an anti-inflammatory phenotype	1025:1087	Our results indicate that HPP@Cu gel could efficiently remove RONS from inflammatory sites and promote repolarization of macrophages to an anti-inflammatory phenotype.					
35526013	6	53	theme	anti-inflammatory	1061:1077	arg1	phenotype					1079:1087	an anti-inflammatory phenotype	1058:1087	an anti-inflammatory phenotype	1058:1087	Our results indicate that HPP@Cu gel could efficiently remove RONS from inflammatory sites and promote repolarization of macrophages to an anti-inflammatory phenotype.					
35526013	3	54	theme	copper	575:580	arg1	NDs					595:597	Cu NDs	592:597	Cu NDs	592:597	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	54	theme	copper	575:580	arg1	nanodots					582:589	copper nanodots	575:589	copper nanodots (Cu NDs)	575:598	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	4	55	theme	free	727:730	arg1	activity					751:758	efficient free radical scavenging activity	717:758	efficient free radical scavenging activity	717:758	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	4	56	theme	novel	644:648	arg1	nano-scavenger					650:663	a novel nano-scavenger	642:663	a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity	642:758	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	4	56	theme	novel	644:648	arg1	NDs					635:637	Cu NDs	632:637	Cu NDs	632:637	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	4	57	theme	oxygen	677:682	arg1	nano-scavenger					650:663	a novel nano-scavenger	642:663	a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity	642:758	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	4	57	theme	oxygen	677:682	arg1	NDs					635:637	Cu NDs	632:637	Cu NDs	632:637	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	4	58	theme	species	697:703	arg1	nano-scavenger					650:663	a novel nano-scavenger	642:663	a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity	642:758	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	4	58	theme	species	697:703	arg1	NDs					635:637	Cu NDs	632:637	Cu NDs	632:637	Cu NDs is a novel nano-scavenger of reactive oxygen and nitrogen species (RONS) with efficient free radical scavenging activity.					
35526013	5	59	theme	drug	909:912	arg1	action					914:919	drug action	909:919	drug action	909:919	HPP@Cu gel is injected into the articular cavity, where it form an in situ gel that slowly released Cu NDs, HA, and PRP, prolonging the duration of drug action.					
35526013	5	60	theme	Cu	861:862	arg1	NDs					864:866	Cu NDs	861:866	Cu NDs	861:866	HPP@Cu gel is injected into the articular cavity, where it form an in situ gel that slowly released Cu NDs, HA, and PRP, prolonging the duration of drug action.					
35526013	7	61	from	production	1180:1189	arg1	rats					1197:1200	OA rats	1194:1200	OA rats	1194:1200	The HPP@Cu gel therapy dramatically reduces cartilage degradation and inflammatory factor production in OA rats.					
35526013	3	62	theme	hyaluronic	500:509	arg1	HA					517:518	HA	517:518	HA	517:518	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	62	theme	hyaluronic	500:509	arg1	acid					511:514	hyaluronic acid	500:514	hyaluronic acid (HA) mixture	500:527	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	8	63	theme	reliable	1225:1232	arg1	reference					1234:1242	a reliable reference	1223:1242	a reliable reference for the application of injectable hydrogels in inflammatory diseases associated with oxidative stress	1223:1344	This study provides a reliable reference for the application of injectable hydrogels in inflammatory diseases associated with oxidative stress.					
35526013	8	64	theme	injectable	1267:1276	arg1	hydrogels					1278:1286	injectable hydrogels	1267:1286	injectable hydrogels	1267:1286	This study provides a reliable reference for the application of injectable hydrogels in inflammatory diseases associated with oxidative stress.					
35526013	3	65	theme	platelet-rich	604:616	arg1	PRP					626:628	PRP	626:628	PRP	626:628	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	65	theme	platelet-rich	604:616	arg1	plasma					618:623	platelet-rich plasma	604:623	platelet-rich plasma (PRP)	604:629	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	2	66	theme	loop	271:274	arg1	system					276:281	the complex malignant feedback loop system	240:281	the complex malignant feedback loop system dominated by oxidative stress and pro-inflammatory factors	240:340	Blocking the complex malignant feedback loop system dominated by oxidative stress and pro-inflammatory factors is the key to treating OA.					
35526013	5	67	theme	articular	793:801	arg1	cavity					803:808	the articular cavity	789:808	the articular cavity	789:808	HPP@Cu gel is injected into the articular cavity, where it form an in situ gel that slowly released Cu NDs, HA, and PRP, prolonging the duration of drug action.					
35526013	7	68	theme	HPP	1094:1096	arg1	therapy					1105:1111	The HPP@Cu gel therapy	1090:1111	The HPP@Cu gel therapy	1090:1111	The HPP@Cu gel therapy dramatically reduces cartilage degradation and inflammatory factor production in OA rats.					
35526013	2	69	theme	feedback	262:269	arg1	system					276:281	the complex malignant feedback loop system	240:281	the complex malignant feedback loop system dominated by oxidative stress and pro-inflammatory factors	240:340	Blocking the complex malignant feedback loop system dominated by oxidative stress and pro-inflammatory factors is the key to treating OA.					
35526013	3	70	theme	multifunctional	388:402	arg1	gel					448:450	HPP@Cu gel	441:450	HPP@Cu gel	441:450	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	70	theme	multifunctional	388:402	arg1	hydrogel					431:438	a multifunctional composite thermo-sensitive hydrogel	386:438	a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel)	386:451	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	3	70	theme	multifunctional	388:402	arg1	matrix					540:545	the gel matrix	532:545	the gel matrix	532:545	Here, we develop a multifunctional composite thermo-sensitive hydrogel (HPP@Cu gel), which is utilized by Poloxamer 407 (P407) and hyaluronic acid (HA) mixture as the gel matrix, then physically mixed with copper nanodots (Cu NDs) and platelet-rich plasma (PRP).					
35526013	7	71	theme	@	1097:1097	arg1	therapy					1105:1111	The HPP@Cu gel therapy	1090:1111	The HPP@Cu gel therapy	1090:1111	The HPP@Cu gel therapy dramatically reduces cartilage degradation and inflammatory factor production in OA rats.					
35028784	0	0	theme	Intact	71:76	arg1	Wall					93:96	the Intact Porcine Aortic Wall	67:96	the Intact Porcine Aortic Wall	67:96	Intramural Distributions of GAGs and Collagen vs. Opening Angle of the Intact Porcine Aortic Wall.					
35028784	3	1	theme	mean	636:639	arg1	radius					641:646	the aortic ring's mean radius	618:646	the aortic ring's mean radius	618:646	The opening angle correlated positively with the aortic ring's mean radius and thickness, with good and moderate correlations respectively.					
35028784	7	2	theme	individual	1309:1318	arg1	role					1320:1323	the individual role	1305:1323	the individual role sGAG play through their content and intramural gradient	1305:1379	We propose that, in addition to the individual role sGAG play through their content and intramural gradient, the interaction between collagen and sGAG should be considered when evaluating circumferential residual stresses in the aorta.					
35028784	6	3	theme	collagen	1194:1201	arg1	gradients					1181:1189	the intramural gradients	1166:1189	the intramural gradients of collagen, sGAG and collagen:sGAG	1166:1225	Additionally, the intramural gradients of collagen, sGAG and collagen:sGAG correlated moderately with the opening angle.					
35028784	3	4	theme	aortic	622:627	arg1	radius					641:646	the aortic ring's mean radius	618:646	the aortic ring's mean radius	618:646	The opening angle correlated positively with the aortic ring's mean radius and thickness, with good and moderate correlations respectively.					
35028784	4	5	theme	sGAG	780:783	arg1	ratio					785:789	the sGAG, collagen, elastin, and collagen:sGAG ratio	738:789	the sGAG, collagen, elastin, and collagen:sGAG ratio	738:789	The correlations between the sGAG, collagen, elastin, and collagen:sGAG ratio and the opening angle were evaluated to identify aortic compositional factors that could play roles in regulating circumferential residual stresses.					
35028784	1	6	theme	elastin	150:156	arg1	contribution					121:132	contribution	121:132	contribution	121:132	The heterogeneity and contribution of collagen and elastin to residual stresses have been thoroughly studied, but more recently, glycosaminoglycans (GAGs) also emerged as potential regulators.					
35028784	1	6	theme	elastin	150:156	arg1	heterogeneity					103:115	heterogeneity	103:115	heterogeneity	103:115	The heterogeneity and contribution of collagen and elastin to residual stresses have been thoroughly studied, but more recently, glycosaminoglycans (GAGs) also emerged as potential regulators.					
35028784	0	7	theme	Aortic	86:91	arg1	Wall					93:96	the Intact Porcine Aortic Wall	67:96	the Intact Porcine Aortic Wall	67:96	Intramural Distributions of GAGs and Collagen vs. Opening Angle of the Intact Porcine Aortic Wall.					
35028784	5	8	theme	<	1045:1045	arg1	r					1030:1030	r	1030:1030	r = - 0.715	1030:1040	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	5	8	theme	<	1045:1045	arg1	p					1043:1043	p < 0.001	1043:1051	p < 0.001	1043:1051	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	2	9	theme	thoracic	529:536	arg1	regions					538:544	the ascending, aortic arch and descending thoracic regions	487:544	the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs	487:570	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	0	10	theme	Porcine	78:84	arg1	Wall					93:96	the Intact Porcine Aortic Wall	67:96	the Intact Porcine Aortic Wall	67:96	Intramural Distributions of GAGs and Collagen vs. Opening Angle of the Intact Porcine Aortic Wall.					
35028784	2	11	theme	descending	518:527	arg1	regions					538:544	the ascending, aortic arch and descending thoracic regions	487:544	the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs	487:570	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	4	12	theme	residual	921:928	arg1	stresses					930:937	circumferential residual stresses	905:937	circumferential residual stresses	905:937	The correlations between the sGAG, collagen, elastin, and collagen:sGAG ratio and the opening angle were evaluated to identify aortic compositional factors that could play roles in regulating circumferential residual stresses.					
35028784	2	13	theme	pigs	567:570	arg1	regions					538:544	the ascending, aortic arch and descending thoracic regions	487:544	the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs	487:570	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	2	14	dep	rings	335:339	arg1	indicator					345:353	an indicator	342:353	an indicator of circumferential residual stresses	342:390	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	0	15	theme	Wall	93:96	arg1	Collagen					37:44	Collagen	37:44	Collagen	37:44	Intramural Distributions of GAGs and Collagen vs. Opening Angle of the Intact Porcine Aortic Wall.					
35028784	0	15	theme	Wall	93:96	arg1	Angle					58:62	Opening Angle	50:62	Opening Angle	50:62	Intramural Distributions of GAGs and Collagen vs. Opening Angle of the Intact Porcine Aortic Wall.					
35028784	0	15	theme	Wall	93:96	arg1	Distributions					11:23	Intramural Distributions	0:23	Intramural Distributions of GAGs	0:31	Intramural Distributions of GAGs and Collagen vs. Opening Angle of the Intact Porcine Aortic Wall.					
35028784	2	16	theme	6-month-old	555:565	arg1	pigs					567:570	5- to 6-month-old pigs	549:570	5- to 6-month-old pigs	549:570	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	1	17	dep	heterogeneity	103:115	arg1	The					99:101	The	99:101	The	99:101	The heterogeneity and contribution of collagen and elastin to residual stresses have been thoroughly studied, but more recently, glycosaminoglycans (GAGs) also emerged as potential regulators.					
35028784	1	18	theme	residual	161:168	arg1	stresses					170:177	residual stresses	161:177	residual stresses	161:177	The heterogeneity and contribution of collagen and elastin to residual stresses have been thoroughly studied, but more recently, glycosaminoglycans (GAGs) also emerged as potential regulators.					
35028784	2	19	theme	distributions	407:419	arg1	angle					319:323	the opening angle	307:323	the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin	307:466	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	3	20	theme	good	668:671	arg1	correlations					686:697	good and moderate correlations	668:697	good and moderate correlations respectively	668:710	The opening angle correlated positively with the aortic ring's mean radius and thickness, with good and moderate correlations respectively.					
35028784	4	21	theme	opening	799:805	arg1	angle					807:811	the opening angle	795:811	the opening angle	795:811	The correlations between the sGAG, collagen, elastin, and collagen:sGAG ratio and the opening angle were evaluated to identify aortic compositional factors that could play roles in regulating circumferential residual stresses.					
35028784	0	22	theme	Intramural	0:9	arg1	Distributions					11:23	Intramural Distributions	0:23	Intramural Distributions of GAGs	0:31	Intramural Distributions of GAGs and Collagen vs. Opening Angle of the Intact Porcine Aortic Wall.					
35028784	5	23	dep	=	1131:1131	arg1	0.623					1133:1137	0.623	1133:1137	0.623	1133:1137	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	7	24	from	stresses	1486:1493	arg1	aorta					1502:1506	the aorta	1498:1506	the aorta	1498:1506	We propose that, in addition to the individual role sGAG play through their content and intramural gradient, the interaction between collagen and sGAG should be considered when evaluating circumferential residual stresses in the aorta.					
35028784	0	25	theme	GAGs	28:31	arg1	Collagen					37:44	Collagen	37:44	Collagen	37:44	Intramural Distributions of GAGs and Collagen vs. Opening Angle of the Intact Porcine Aortic Wall.					
35028784	0	25	theme	GAGs	28:31	arg1	Angle					58:62	Opening Angle	50:62	Opening Angle	50:62	Intramural Distributions of GAGs and Collagen vs. Opening Angle of the Intact Porcine Aortic Wall.					
35028784	0	25	theme	GAGs	28:31	arg1	Distributions					11:23	Intramural Distributions	0:23	Intramural Distributions of GAGs	0:31	Intramural Distributions of GAGs and Collagen vs. Opening Angle of the Intact Porcine Aortic Wall.					
35028784	3	26	theme	moderate	677:684	arg1	correlations					686:697	good and moderate correlations	668:697	good and moderate correlations respectively	668:710	The opening angle correlated positively with the aortic ring's mean radius and thickness, with good and moderate correlations respectively.					
35028784	2	27	theme	ascending	491:499	arg1	regions					538:544	the ascending, aortic arch and descending thoracic regions	487:544	the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs	487:570	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	6	28	theme	sGAG	1204:1207	arg1	gradients					1181:1189	the intramural gradients	1166:1189	the intramural gradients of collagen, sGAG and collagen:sGAG	1166:1225	Additionally, the intramural gradients of collagen, sGAG and collagen:sGAG correlated moderately with the opening angle.					
35028784	2	29	dep	6-month-old	555:565	arg1	to					552:553	to	552:553	to	552:553	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	5	30	theme	total	1071:1075	arg1	content					1082:1088	the total sGAG content	1067:1088	the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001)	1067:1149	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	2	31	theme	mural	401:405	arg1	distributions					407:419	the mural distributions	397:419	the mural distributions of sulfated GAGs (sGAG), collagen, and elastin	397:466	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	3	32	theme	opening	577:583	arg1	angle					585:589	The opening angle	573:589	The opening angle	573:589	The opening angle correlated positively with the aortic ring's mean radius and thickness, with good and moderate correlations respectively.					
35028784	5	33	with	correlation	994:1004	arg1	angle					1023:1027	the opening angle	1011:1027	the opening angle	1011:1027	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	2	34	theme	stresses	383:390	arg1	indicator					345:353	an indicator	342:353	an indicator of circumferential residual stresses	342:390	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	5	35	theme	sGAG	1077:1080	arg1	content					1082:1088	the total sGAG content	1067:1088	the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001)	1067:1149	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	1	36	theme	potential	270:278	arg1	regulators					280:289	potential regulators	270:289	potential regulators	270:289	The heterogeneity and contribution of collagen and elastin to residual stresses have been thoroughly studied, but more recently, glycosaminoglycans (GAGs) also emerged as potential regulators.					
35028784	4	37	theme	compositional	847:859	arg1	factors					861:867	aortic compositional factors	840:867	aortic compositional factors that could play roles in regulating circumferential residual stresses	840:937	The correlations between the sGAG, collagen, elastin, and collagen:sGAG ratio and the opening angle were evaluated to identify aortic compositional factors that could play roles in regulating circumferential residual stresses.					
35028784	5	38	theme	total	944:948	arg1	ratio					964:968	The total collagen:sGAG ratio	940:968	The total collagen:sGAG ratio	940:968	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	2	39	theme	residual	374:381	arg1	stresses					383:390	circumferential residual stresses	358:390	circumferential residual stresses	358:390	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	2	40	theme	GAGs	433:436	arg1	distributions					407:419	the mural distributions	397:419	the mural distributions of sulfated GAGs (sGAG), collagen, and elastin	397:466	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	2	40	theme	GAGs	433:436	arg1	rings					335:339	aortic rings	328:339	aortic rings (an indicator of circumferential residual stresses)	328:391	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	7	41	theme	circumferential	1461:1475	arg1	stresses					1486:1493	circumferential residual stresses	1461:1493	circumferential residual stresses in the aorta	1461:1506	We propose that, in addition to the individual role sGAG play through their content and intramural gradient, the interaction between collagen and sGAG should be considered when evaluating circumferential residual stresses in the aorta.					
35028784	4	42	theme	aortic	840:845	arg1	factors					861:867	aortic compositional factors	840:867	aortic compositional factors that could play roles in regulating circumferential residual stresses	840:937	The correlations between the sGAG, collagen, elastin, and collagen:sGAG ratio and the opening angle were evaluated to identify aortic compositional factors that could play roles in regulating circumferential residual stresses.					
35028784	7	43	theme	intramural	1361:1370	arg1	gradient					1372:1379	intramural gradient	1361:1379	intramural gradient	1361:1379	We propose that, in addition to the individual role sGAG play through their content and intramural gradient, the interaction between collagen and sGAG should be considered when evaluating circumferential residual stresses in the aorta.					
35028784	5	44	theme	collagen	950:957	arg1	ratio					964:968	The total collagen:sGAG ratio	940:968	The total collagen:sGAG ratio	940:968	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	2	45	theme	circumferential	358:372	arg1	stresses					383:390	circumferential residual stresses	358:390	circumferential residual stresses	358:390	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	0	46	theme	Opening	50:56	arg1	Angle					58:62	Opening Angle	50:62	Opening Angle	50:62	Intramural Distributions of GAGs and Collagen vs. Opening Angle of the Intact Porcine Aortic Wall.					
35028784	7	47	theme	residual	1477:1484	arg1	stresses					1486:1493	circumferential residual stresses	1461:1493	circumferential residual stresses in the aorta	1461:1506	We propose that, in addition to the individual role sGAG play through their content and intramural gradient, the interaction between collagen and sGAG should be considered when evaluating circumferential residual stresses in the aorta.					
35028784	6	48	theme	intramural	1170:1179	arg1	gradients					1181:1189	the intramural gradients	1166:1189	the intramural gradients of collagen, sGAG and collagen:sGAG	1166:1225	Additionally, the intramural gradients of collagen, sGAG and collagen:sGAG correlated moderately with the opening angle.					
35028784	5	49	theme	opening	1015:1021	arg1	angle					1023:1027	the opening angle	1011:1027	the opening angle	1011:1027	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	2	50	theme	opening	311:317	arg1	angle					319:323	the opening angle	307:323	the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin	307:466	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	6	51	dep	collagen	1194:1201	arg1	sGAG					1222:1225	sGAG	1222:1225	collagen, sGAG and collagen:sGAG	1194:1225	Additionally, the intramural gradients of collagen, sGAG and collagen:sGAG correlated moderately with the opening angle.					
35028784	6	52	theme	opening	1258:1264	arg1	angle					1266:1270	the opening angle	1254:1270	the opening angle	1254:1270	Additionally, the intramural gradients of collagen, sGAG and collagen:sGAG correlated moderately with the opening angle.					
35028784	2	53	theme	arch	509:512	arg1	regions					538:544	the ascending, aortic arch and descending thoracic regions	487:544	the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs	487:570	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	5	54	theme	sGAG	959:962	arg1	ratio					964:968	The total collagen:sGAG ratio	940:968	The total collagen:sGAG ratio	940:968	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	7	55	dep	role	1320:1323	arg1	play					1330:1333	play	1330:1333	play through their content and intramural gradient	1330:1379	We propose that, in addition to the individual role sGAG play through their content and intramural gradient, the interaction between collagen and sGAG should be considered when evaluating circumferential residual stresses in the aorta.					
35028784	5	56	theme	<	1142:1142	arg1	p					1140:1140	p < 0.001	1140:1148	p < 0.001	1140:1148	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	5	56	theme	<	1142:1142	arg1	r					1129:1129	r	1129:1129	r = 0.623	1129:1137	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	5	57	theme	good	1111:1114	arg1	correlation					1116:1126	a good correlation	1109:1126	a good correlation (r = 0.623, p < 0.001)	1109:1149	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	1	58	gly	heterogeneity	103:115	arg1	collagen					137:144	collagen	137:144	collagen	137:144	The heterogeneity and contribution of collagen and elastin to residual stresses have been thoroughly studied, but more recently, glycosaminoglycans (GAGs) also emerged as potential regulators.					
35028784	1	58	gly	heterogeneity	103:115	arg1	elastin					150:156	elastin	150:156	elastin	150:156	The heterogeneity and contribution of collagen and elastin to residual stresses have been thoroughly studied, but more recently, glycosaminoglycans (GAGs) also emerged as potential regulators.					
35028784	2	59	theme	aortic	328:333	arg1	rings					335:339	aortic rings	328:339	aortic rings (an indicator of circumferential residual stresses)	328:391	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	5	60	dep	correlation	1116:1126	arg1	p					1140:1140	p < 0.001	1140:1148	p < 0.001	1140:1148	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	5	60	dep	correlation	1116:1126	arg1	r					1129:1129	r	1129:1129	r = 0.623	1129:1137	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	5	61	theme	strongest	984:992	arg1	correlation					994:1004	the strongest correlation	980:1004	the strongest correlation with the opening angle (r = - 0.715, p < 0.001)	980:1052	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	4	62	theme	sGAG	742:745	arg1	ratio					785:789	the sGAG, collagen, elastin, and collagen:sGAG ratio	738:789	the sGAG, collagen, elastin, and collagen:sGAG ratio	738:789	The correlations between the sGAG, collagen, elastin, and collagen:sGAG ratio and the opening angle were evaluated to identify aortic compositional factors that could play roles in regulating circumferential residual stresses.					
35028784	2	63	theme	rings	335:339	arg1	angle					319:323	the opening angle	307:323	the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin	307:466	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	5	64	dep	correlation	994:1004	arg1	r					1030:1030	r	1030:1030	r = - 0.715	1030:1040	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	5	64	dep	correlation	994:1004	arg1	p					1043:1043	p < 0.001	1043:1051	p < 0.001	1043:1051	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	2	65	theme	sulfated	424:431	arg1	elastin					460:466	elastin	460:466	elastin	460:466	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	2	65	theme	sulfated	424:431	arg1	sGAG					439:442	sGAG	439:442	sGAG	439:442	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	2	65	theme	sulfated	424:431	arg1	collagen					446:453	collagen	446:453	collagen	446:453	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	2	65	theme	sulfated	424:431	arg1	GAGs					433:436	sulfated GAGs	424:436	sulfated GAGs (sGAG)	424:443	In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs.					
35028784	4	66	theme	circumferential	905:919	arg1	stresses					930:937	circumferential residual stresses	905:937	circumferential residual stresses	905:937	The correlations between the sGAG, collagen, elastin, and collagen:sGAG ratio and the opening angle were evaluated to identify aortic compositional factors that could play roles in regulating circumferential residual stresses.					
35028784	1	67	theme	collagen	137:144	arg1	contribution					121:132	contribution	121:132	contribution	121:132	The heterogeneity and contribution of collagen and elastin to residual stresses have been thoroughly studied, but more recently, glycosaminoglycans (GAGs) also emerged as potential regulators.					
35028784	1	67	theme	collagen	137:144	arg1	heterogeneity					103:115	heterogeneity	103:115	heterogeneity	103:115	The heterogeneity and contribution of collagen and elastin to residual stresses have been thoroughly studied, but more recently, glycosaminoglycans (GAGs) also emerged as potential regulators.					
35028784	5	68	theme	=	1131:1131	arg1	p					1140:1140	p < 0.001	1140:1148	p < 0.001	1140:1148	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	5	68	theme	=	1131:1131	arg1	r					1129:1129	r	1129:1129	r = 0.623	1129:1137	The total collagen:sGAG ratio displayed the strongest correlation with the opening angle (r = - 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation (r = 0.623, p < 0.001).					
35028784	6	69	theme	collagen	1213:1220	arg1	gradients					1181:1189	the intramural gradients	1166:1189	the intramural gradients of collagen, sGAG and collagen:sGAG	1166:1225	Additionally, the intramural gradients of collagen, sGAG and collagen:sGAG correlated moderately with the opening angle.					
35337687	7	0	theme	sound	1086:1090	arg1	properties					1074:1083	the PVA gel acoustic properties	1053:1083	the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm)	1053:1183	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	7	0	theme	sound	1086:1090	arg1	speed					1092:1096	sound speed	1086:1096	sound speed	1086:1096	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	7	0	theme	sound	1086:1090	arg1	coefficient					1138:1148	attenuation coefficient	1126:1148	attenuation coefficient	1126:1148	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	9	1	theme	ultrasound	1536:1545	arg1	applications					1547:1558	therapeutic ultrasound applications	1524:1558	therapeutic ultrasound applications	1524:1558	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	7	2	dep	speed	1092:1096	arg1	m/s					1121:1123	1532 ± 17 to 1590 ± 9 m/s	1099:1123	1532 ± 17 to 1590 ± 9 m/s	1099:1123	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	7	2	dep	speed	1092:1096	arg1	±					1156:1156	0.08 ± 0.01 to 0.37 ± 0.02 dB/cm	1151:1182	0.08 ± 0.01 to 0.37 ± 0.02 dB/cm	1151:1182	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	7	3	theme	±	1104:1104	arg1	m/s					1121:1123	1532 ± 17 to 1590 ± 9 m/s	1099:1123	1532 ± 17 to 1590 ± 9 m/s	1099:1123	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	9	4	theme	tissue	1501:1506	arg1	cavitation					1459:1468	cavitation	1459:1468	cavitation	1459:1468	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	9	4	theme	tissue	1501:1506	arg1	properties					1482:1491	thermal properties	1474:1491	thermal properties	1474:1491	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	9	4	theme	tissue	1501:1506	arg1	acoustic					1449:1456	acoustic	1449:1456	acoustic	1449:1456	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	8	5	theme	%	1257:1257	arg1	hydrogels					1263:1271	10% PVA hydrogels	1255:1271	10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa)	1255:1330	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	0	6	theme	Poly	68:71	arg1	Hydrogels					88:96	Poly(vinyl alcohol) Hydrogels	68:96	Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics	68:144	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	7	7	dep	dB/cm	1178:1182	arg1	to					1163:1164	to	1163:1164	to	1163:1164	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	9	8	from	summary	1380:1386	arg1	suitable					1430:1437	suitable	1430:1437	suitable	1430:1437	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	0	9	theme	alcohol	79:85	arg1	Hydrogels					88:96	Poly(vinyl alcohol) Hydrogels	68:96	Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics	68:144	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	8	10	theme	Cavitation	1229:1238	arg1	thresholds					1240:1249	Cavitation thresholds	1229:1249	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa)	1229:1330	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	5	11	theme	fresh	869:873	arg1	tissue					881:886	fresh sheep tissue	869:886	fresh sheep tissue (kidney, liver, spleen)	869:910	Results were compared with measurements in fresh sheep tissue (kidney, liver, spleen).					
35337687	7	12	theme	fresh	1215:1219	arg1	tissue					1221:1226	fresh tissue	1215:1226	fresh tissue	1215:1226	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	1	13	theme	therapeutic	235:245	arg1	applications					247:258	therapeutic applications	235:258	therapeutic applications	235:258	The thermal and mechanical effects induced in tissue by ultrasound can be exploited for therapeutic applications.					
35337687	0	14	theme	vinyl	73:77	arg1	Hydrogels					88:96	Poly(vinyl alcohol) Hydrogels	68:96	Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics	68:144	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	8	15	dep	MPa	1298:1300	arg1	MPa					1327:1329	5.4-8.2 MPa	1319:1329	5.4-8.2 MPa	1319:1329	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	5	16	theme	sheep	875:879	arg1	tissue					881:886	fresh sheep tissue	869:886	fresh sheep tissue (kidney, liver, spleen)	869:910	Results were compared with measurements in fresh sheep tissue (kidney, liver, spleen).					
35337687	6	17	theme	cellulose	990:998	arg1	content					1000:1006	cellulose content	990:1006	cellulose content	990:1006	Sound speed depended most strongly on PVA concentration, and attenuation, on cellulose content.					
35337687	0	18	theme	Hydrogels	88:96	arg1	Acoustic					20:27	Acoustic	20:27	Acoustic	20:27	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	0	18	theme	Hydrogels	88:96	arg1	Properties					54:63	Thermal Properties	46:63	Thermal Properties	46:63	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	0	18	theme	Hydrogels	88:96	arg1	Cavitation					30:39	Cavitation	30:39	Cavitation	30:39	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	8	19	theme	%	1305:1305	arg1	occurrence					1307:1316	75% occurrence	1303:1316	75% occurrence	1303:1316	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	8	19	theme	%	1305:1305	arg1	MPa					1298:1300	4.1-5.4 MPa	1290:1300	4.1-5.4 MPa	1290:1300	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	2	20	theme	Tissue-mimicking	261:276	arg1	materials					278:286	Tissue-mimicking materials	261:286	Tissue-mimicking materials (TMMs)	261:293	Tissue-mimicking materials (TMMs), reflecting different soft tissue properties, are required for experimental evaluation of therapeutic potential.					
35337687	2	20	theme	Tissue-mimicking	261:276	arg1	TMMs					289:292	TMMs	289:292	TMMs	289:292	Tissue-mimicking materials (TMMs), reflecting different soft tissue properties, are required for experimental evaluation of therapeutic potential.					
35337687	2	21	theme	therapeutic	385:395	arg1	potential					397:405	therapeutic potential	385:405	therapeutic potential	385:405	Tissue-mimicking materials (TMMs), reflecting different soft tissue properties, are required for experimental evaluation of therapeutic potential.					
35337687	7	22	theme	acoustic	1065:1072	arg1	properties					1074:1083	the PVA gel acoustic properties	1053:1083	the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm)	1053:1183	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	7	22	theme	acoustic	1065:1072	arg1	speed					1092:1096	sound speed	1086:1096	sound speed	1086:1096	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	9	23	theme	composite	1403:1411	arg1	hydrogels					1413:1421	PVA cellulose composite hydrogels	1389:1421	PVA cellulose composite hydrogels	1389:1421	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	1	24	theme	thermal	151:157	arg1	effects					174:180	The thermal and mechanical effects	147:180	The thermal and mechanical effects induced in tissue by ultrasound	147:212	The thermal and mechanical effects induced in tissue by ultrasound can be exploited for therapeutic applications.					
35337687	9	25	theme	applications	1547:1558	arg1	number					1514:1519	a number	1512:1519	a number of therapeutic ultrasound applications	1512:1558	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	4	26	theme	heat	766:769	arg1	conductivity					743:754	thermal conductivity	735:754	thermal conductivity	735:754	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	26	theme	heat	766:769	arg1	capacity					771:778	specific heat capacity	757:778	specific heat capacity	757:778	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	6	27	theme	PVA	951:953	arg1	concentration					955:967	PVA concentration	951:967	PVA concentration	951:967	Sound speed depended most strongly on PVA concentration, and attenuation, on cellulose content.					
35337687	8	28	theme	75	1303:1304	arg1	%					1305:1305	%	1305:1305	%	1305:1305	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	4	29	theme	specific	757:764	arg1	conductivity					743:754	thermal conductivity	735:754	thermal conductivity	735:754	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	29	theme	specific	757:764	arg1	capacity					771:778	specific heat capacity	757:778	specific heat capacity	757:778	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	9	30	theme	therapeutic	1524:1534	arg1	applications					1547:1558	therapeutic ultrasound applications	1524:1558	therapeutic ultrasound applications	1524:1558	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	7	31	theme	attenuation	1126:1136	arg1	speed					1092:1096	sound speed	1086:1096	sound speed	1086:1096	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	7	31	theme	attenuation	1126:1136	arg1	coefficient					1138:1148	attenuation coefficient	1126:1148	attenuation coefficient	1126:1148	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	2	32	theme	experimental	358:369	arg1	evaluation					371:380	experimental evaluation	358:380	experimental evaluation of therapeutic potential	358:405	Tissue-mimicking materials (TMMs), reflecting different soft tissue properties, are required for experimental evaluation of therapeutic potential.					
35337687	8	33	dep	occurrence	1278:1287	arg1	occurrence					1307:1316	75% occurrence	1303:1316	75% occurrence	1303:1316	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	8	33	dep	occurrence	1278:1287	arg1	MPa					1298:1300	4.1-5.4 MPa	1290:1300	4.1-5.4 MPa	1290:1300	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	9	34	theme	thermal	1474:1480	arg1	properties					1482:1491	thermal properties	1474:1491	thermal properties	1474:1491	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	9	35	theme	PVA	1389:1391	arg1	hydrogels					1413:1421	PVA cellulose composite hydrogels	1389:1421	PVA cellulose composite hydrogels	1389:1421	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	3	36	theme	vinyl	442:446	arg1	poly					437:440	poly	437:440	poly(vinyl alcohol) (PVA) hydrogels	437:471	In the study described here, poly(vinyl alcohol) (PVA) hydrogels were characterized.					
35337687	3	36	theme	vinyl	442:446	arg1	alcohol					448:454	vinyl alcohol	442:454	vinyl alcohol	442:454	In the study described here, poly(vinyl alcohol) (PVA) hydrogels were characterized.					
35337687	0	37	theme	Acoustic	20:27	arg1	Characterization					0:15	Characterization	0:15	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.	0:145	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	4	38	theme	%	545:545	arg1	%					549:549	5%-20% w/w	544:553	5%-20% w/w	544:553	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	38	theme	%	545:545	arg1	concentrations					528:541	different concentrations	518:541	different concentrations (5%-20% w/w)	518:554	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	39	theme	thresholds	814:823	arg1	terms					788:792	terms	788:792	terms of their cavitation thresholds	788:823	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	8	40	theme	PVA	1259:1261	arg1	hydrogels					1263:1271	10% PVA hydrogels	1255:1271	10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa)	1255:1330	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	4	41	dep	characterized	628:640	arg1	attenuation					669:679	attenuation	669:679	attenuation	669:679	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	41	dep	characterized	628:640	arg1	speed					662:666	sound speed	656:666	sound speed	656:666	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	42	theme	PVA	581:583	arg1	%					616:616	2.5%-10% w/w	609:620	2.5%-10% w/w	609:620	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	42	theme	PVA	581:583	arg1	scatterers					597:606	PVA ± cellulose scatterers	581:606	PVA ± cellulose scatterers (2.5%-10% w/w)	581:621	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	0	43	theme	Therapeutic	109:119	arg1	Mimics					139:144	Therapeutic Ultrasound Tissue Mimics	109:144	Therapeutic Ultrasound Tissue Mimics	109:144	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	4	44	theme	scatterers	597:606	arg1	weights					570:576	molecular weights	560:576	molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w)	560:621	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	44	theme	scatterers	597:606	arg1	%					549:549	5%-20% w/w	544:553	5%-20% w/w	544:553	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	44	theme	scatterers	597:606	arg1	concentrations					528:541	different concentrations	518:541	different concentrations (5%-20% w/w)	518:554	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	1	45	theme	mechanical	163:172	arg1	effects					174:180	The thermal and mechanical effects	147:180	The thermal and mechanical effects induced in tissue by ultrasound	147:212	The thermal and mechanical effects induced in tissue by ultrasound can be exploited for therapeutic applications.					
35337687	3	46	theme	poly	437:440	arg1	hydrogels					463:471	poly(vinyl alcohol) (PVA) hydrogels	437:471	poly(vinyl alcohol) (PVA) hydrogels	437:471	In the study described here, poly(vinyl alcohol) (PVA) hydrogels were characterized.					
35337687	0	47	theme	Cavitation	30:39	arg1	Characterization					0:15	Characterization	0:15	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.	0:145	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	8	48	theme	cellulose	1358:1366	arg1	content					1368:1374	cellulose content	1358:1374	cellulose content	1358:1374	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	7	49	theme	formulations	1026:1037	arg1	range					1017:1021	the range	1013:1021	the range of formulations investigated	1013:1050	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	2	50	theme	potential	397:405	arg1	evaluation					371:380	experimental evaluation	358:380	experimental evaluation of therapeutic potential	358:405	Tissue-mimicking materials (TMMs), reflecting different soft tissue properties, are required for experimental evaluation of therapeutic potential.					
35337687	0	51	theme	Thermal	46:52	arg1	Properties					54:63	Thermal Properties	46:63	Thermal Properties	46:63	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	8	52	dep	hydrogels	1263:1271	arg1	occurrence					1278:1287	50% occurrence	1274:1287	50% occurrence	1274:1287	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	0	53	theme	Tissue	132:137	arg1	Mimics					139:144	Therapeutic Ultrasound Tissue Mimics	109:144	Therapeutic Ultrasound Tissue Mimics	109:144	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	7	54	theme	1532	1099:1102	arg1	m/s					1121:1123	1532 ± 17 to 1590 ± 9 m/s	1099:1123	1532 ± 17 to 1590 ± 9 m/s	1099:1123	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	4	55	theme	thermal	735:741	arg1	conductivity					743:754	thermal conductivity	735:754	thermal conductivity	735:754	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	55	theme	thermal	735:741	arg1	capacity					771:778	specific heat capacity	757:778	specific heat capacity	757:778	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	5	56	dep	tissue	881:886	arg1	liver					897:901	liver	897:901	liver	897:901	Results were compared with measurements in fresh sheep tissue (kidney, liver, spleen).					
35337687	5	56	dep	tissue	881:886	arg1	kidney					889:894	kidney	889:894	kidney	889:894	Results were compared with measurements in fresh sheep tissue (kidney, liver, spleen).					
35337687	5	56	dep	tissue	881:886	arg1	spleen					904:909	spleen	904:909	spleen	904:909	Results were compared with measurements in fresh sheep tissue (kidney, liver, spleen).					
35337687	9	57	theme	soft	1496:1499	arg1	tissue					1501:1506	soft tissue	1496:1506	soft tissue	1496:1506	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	4	58	theme	different	518:526	arg1	%					549:549	5%-20% w/w	544:553	5%-20% w/w	544:553	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	58	theme	different	518:526	arg1	concentrations					528:541	different concentrations	518:541	different concentrations (5%-20% w/w)	518:554	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	0	59	theme	Ultrasound	121:130	arg1	Mimics					139:144	Therapeutic Ultrasound Tissue Mimics	109:144	Therapeutic Ultrasound Tissue Mimics	109:144	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	8	60	theme	%	1276:1276	arg1	occurrence					1278:1287	50% occurrence	1274:1287	50% occurrence	1274:1287	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	8	61	theme	50	1274:1275	arg1	%					1276:1276	%	1276:1276	%	1276:1276	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	2	62	theme	tissue	322:327	arg1	properties					329:338	different soft tissue properties	307:338	different soft tissue properties	307:338	Tissue-mimicking materials (TMMs), reflecting different soft tissue properties, are required for experimental evaluation of therapeutic potential.					
35337687	5	63	from	measurements	853:864	arg1	tissue					881:886	fresh sheep tissue	869:886	fresh sheep tissue (kidney, liver, spleen)	869:910	Results were compared with measurements in fresh sheep tissue (kidney, liver, spleen).					
35337687	9	64	from	suitable	1430:1437	arg1	summary					1380:1386	summary	1380:1386	summary	1380:1386	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	4	65	theme	cavitation	803:812	arg1	thresholds					814:823	their cavitation thresholds	797:823	their cavitation thresholds	797:823	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	66	theme	%	612:612	arg1	%					616:616	2.5%-10% w/w	609:620	2.5%-10% w/w	609:620	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	66	theme	%	612:612	arg1	scatterers					597:606	PVA ± cellulose scatterers	581:606	PVA ± cellulose scatterers (2.5%-10% w/w)	581:621	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	9	67	theme	cellulose	1393:1401	arg1	hydrogels					1413:1421	PVA cellulose composite hydrogels	1389:1421	PVA cellulose composite hydrogels	1389:1421	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	2	68	theme	soft	317:320	arg1	properties					329:338	different soft tissue properties	307:338	different soft tissue properties	307:338	Tissue-mimicking materials (TMMs), reflecting different soft tissue properties, are required for experimental evaluation of therapeutic potential.					
35337687	4	69	theme	molecular	560:568	arg1	weights					570:576	molecular weights	560:576	molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w)	560:621	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	70	theme	temperature	699:709	arg1	Hydrogels					493:501	Hydrogels	493:501	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w)	493:621	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	70	theme	temperature	699:709	arg1	function					687:694	a function	685:694	a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds	685:823	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	2	71	theme	different	307:315	arg1	properties					329:338	different soft tissue properties	307:338	different soft tissue properties	307:338	Tissue-mimicking materials (TMMs), reflecting different soft tissue properties, are required for experimental evaluation of therapeutic potential.					
35337687	4	72	theme	sound	656:660	arg1	attenuation					669:679	attenuation	669:679	attenuation	669:679	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	72	theme	sound	656:660	arg1	speed					662:666	sound speed	656:666	sound speed	656:666	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	7	73	dep	m/s	1121:1123	arg1	to					1109:1110	to	1109:1110	to	1109:1110	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	4	74	theme	cellulose	587:595	arg1	%					616:616	2.5%-10% w/w	609:620	2.5%-10% w/w	609:620	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	74	theme	cellulose	587:595	arg1	scatterers					597:606	PVA ± cellulose scatterers	581:606	PVA ± cellulose scatterers (2.5%-10% w/w)	581:621	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	8	75	theme	10	1255:1256	arg1	%					1257:1257	%	1257:1257	%	1257:1257	Cavitation thresholds for 10% PVA hydrogels (50% occurrence: 4.1-5.4 MPa, 75% occurrence: 5.4-8.2 MPa) decreased with increasing cellulose content.					
35337687	4	76	theme	±	585:585	arg1	%					616:616	2.5%-10% w/w	609:620	2.5%-10% w/w	609:620	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	76	theme	±	585:585	arg1	scatterers					597:606	PVA ± cellulose scatterers	581:606	PVA ± cellulose scatterers (2.5%-10% w/w)	581:621	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	77	dep	thermally	724:732	arg1	conductivity					743:754	thermal conductivity	735:754	thermal conductivity	735:754	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	4	77	dep	thermally	724:732	arg1	capacity					771:778	specific heat capacity	757:778	specific heat capacity	757:778	Hydrogels prepared using different concentrations (5%-20% w/w) and molecular weights of PVA ± cellulose scatterers (2.5%-10% w/w) were characterized acoustically (sound speed, attenuation) as a function of temperature (25°C-45°C), thermally (thermal conductivity, specific heat capacity) and in terms of their cavitation thresholds.					
35337687	0	78	theme	Properties	54:63	arg1	Characterization					0:15	Characterization	0:15	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.	0:145	Characterization of Acoustic, Cavitation, and Thermal Properties of Poly(vinyl alcohol) Hydrogels for Use as Therapeutic Ultrasound Tissue Mimics.					
35337687	9	79	dep	suitable	1430:1437	arg1	mimics					1439:1444	mimics	1439:1444	mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications	1439:1558	In summary, PVA cellulose composite hydrogels may be suitable mimics of acoustic, cavitation and thermal properties of soft tissue for a number of therapeutic ultrasound applications.					
35337687	7	80	theme	±	1117:1117	arg1	m/s					1121:1123	1532 ± 17 to 1590 ± 9 m/s	1099:1123	1532 ± 17 to 1590 ± 9 m/s	1099:1123	For the range of formulations investigated, the PVA gel acoustic properties (sound speed: 1532 ± 17 to 1590 ± 9 m/s, attenuation coefficient: 0.08 ± 0.01 to 0.37 ± 0.02 dB/cm) fell within those measured in fresh tissue.					
35337687	6	81	theme	Sound	913:917	arg1	speed					919:923	Sound speed	913:923	Sound speed	913:923	Sound speed depended most strongly on PVA concentration, and attenuation, on cellulose content.					
37207756	6	0	from	composition	902:912	arg1	mice					951:954	diabetic mice	942:954	diabetic mice	942:954	In addition, 16S rRNA analysis showed that MGGP changed the composition of intestinal microbiota in diabetic mice, decreased the abundance of Proteobacteria, and increased the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae.					
37207756	6	1	theme	Proteobacteria	984:997	arg1	abundance					971:979	the abundance	967:979	the abundance of Proteobacteria	967:997	In addition, 16S rRNA analysis showed that MGGP changed the composition of intestinal microbiota in diabetic mice, decreased the abundance of Proteobacteria, and increased the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae.					
37207756	7	2	theme	gut	1127:1129	arg1	microbiome					1131:1140	the gut microbiome	1123:1140	the gut microbiome	1123:1140	The phenotypes of the gut microbiome also changed accordingly, indicating that MGGP can inhibit the growth of pathogenic bacteria, alleviate intestinal functional metabolic disorders and reverse the potential risk of related complications.					
37207756	0	3	theme	diabetic	77:84	arg1	mice					86:89	type 2 diabetic mice	70:89	type 2 diabetic mice	70:89	Anti-diabetic effect of modified 'Guanximiyou' pummelo peel pectin on type 2 diabetic mice via gut microbiota.					
37207756	5	4	contain	has	761:763	arg1	MGGP					679:682	MGGP	679:682	MGGP	679:682	However, MGGP can more effectively reduce blood glucose and regulate lipid metabolism, and has significant antioxidant capacity and the ability to promote SCFAs secretion.					
37207756	5	4	contain	has	761:763	arg2	capacity					789:796	significant antioxidant capacity	765:796	significant antioxidant capacity	765:796	However, MGGP can more effectively reduce blood glucose and regulate lipid metabolism, and has significant antioxidant capacity and the ability to promote SCFAs secretion.					
37207756	5	4	contain	has	761:763	arg2	ability					806:812	the ability	802:812	the ability to promote SCFAs secretion	802:839	However, MGGP can more effectively reduce blood glucose and regulate lipid metabolism, and has significant antioxidant capacity and the ability to promote SCFAs secretion.					
37207756	3	5	theme	made	441:444	arg1	MGGP					446:449	These made MGGP	435:449	These made MGGP	435:449	These made MGGP have stronger antioxidant capacity and better inhibition effect on corn starch digestion in vitro.					
37207756	7	6	theme	related	1322:1328	arg1	complications					1330:1342	related complications	1322:1342	related complications	1322:1342	The phenotypes of the gut microbiome also changed accordingly, indicating that MGGP can inhibit the growth of pathogenic bacteria, alleviate intestinal functional metabolic disorders and reverse the potential risk of related complications.					
37207756	4	7	theme	diabetes	633:640	arg1	development					618:628	the development	614:628	the development of diabetes	614:640	In vivo experiments have shown that both GGP and MGGP inhibited the development of diabetes after 4 weeks of ingestion.					
37207756	5	8	theme	lipid	739:743	arg1	metabolism					745:754	lipid metabolism	739:754	lipid metabolism	739:754	However, MGGP can more effectively reduce blood glucose and regulate lipid metabolism, and has significant antioxidant capacity and the ability to promote SCFAs secretion.					
37207756	2	9	theme	acid	415:418	arg1	content					391:397	the content	387:397	the content of galacturonic acid	387:418	After modification, pectin was transformed from high methoxy pectin (HMP) to low methoxy pectin (LMP), and the content of galacturonic acid was increased.					
37207756	6	10	theme	diabetic	942:949	arg1	mice					951:954	diabetic mice	942:954	diabetic mice	942:954	In addition, 16S rRNA analysis showed that MGGP changed the composition of intestinal microbiota in diabetic mice, decreased the abundance of Proteobacteria, and increased the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae.					
37207756	6	11	theme	Oscillospirales	1068:1082	arg1	abundance					1027:1035	the relative abundance	1014:1035	the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae	1014:1102	In addition, 16S rRNA analysis showed that MGGP changed the composition of intestinal microbiota in diabetic mice, decreased the abundance of Proteobacteria, and increased the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae.					
37207756	2	12	theme	methoxy	333:339	arg1	HMP					349:351	HMP	349:351	HMP	349:351	After modification, pectin was transformed from high methoxy pectin (HMP) to low methoxy pectin (LMP), and the content of galacturonic acid was increased.					
37207756	2	12	theme	methoxy	333:339	arg1	pectin					341:346	high methoxy pectin	328:346	high methoxy pectin (HMP) to low methoxy pectin (LMP)	328:380	After modification, pectin was transformed from high methoxy pectin (HMP) to low methoxy pectin (LMP), and the content of galacturonic acid was increased.					
37207756	2	13	theme	galacturonic	402:413	arg1	acid					415:418	galacturonic acid	402:418	galacturonic acid	402:418	After modification, pectin was transformed from high methoxy pectin (HMP) to low methoxy pectin (LMP), and the content of galacturonic acid was increased.					
37207756	7	14	theme	pathogenic	1215:1224	arg1	bacteria					1226:1233	pathogenic bacteria	1215:1233	pathogenic bacteria	1215:1233	The phenotypes of the gut microbiome also changed accordingly, indicating that MGGP can inhibit the growth of pathogenic bacteria, alleviate intestinal functional metabolic disorders and reverse the potential risk of related complications.					
37207756	2	15	theme	methoxy	361:367	arg1	LMP					377:379	LMP	377:379	LMP	377:379	After modification, pectin was transformed from high methoxy pectin (HMP) to low methoxy pectin (LMP), and the content of galacturonic acid was increased.					
37207756	2	15	theme	methoxy	361:367	arg1	pectin					369:374	low methoxy pectin	357:374	low methoxy pectin (LMP)	357:380	After modification, pectin was transformed from high methoxy pectin (HMP) to low methoxy pectin (LMP), and the content of galacturonic acid was increased.					
37207756	2	16	theme	high	328:331	arg1	HMP					349:351	HMP	349:351	HMP	349:351	After modification, pectin was transformed from high methoxy pectin (HMP) to low methoxy pectin (LMP), and the content of galacturonic acid was increased.					
37207756	2	16	theme	high	328:331	arg1	pectin					341:346	high methoxy pectin	328:346	high methoxy pectin (HMP) to low methoxy pectin (LMP)	328:380	After modification, pectin was transformed from high methoxy pectin (HMP) to low methoxy pectin (LMP), and the content of galacturonic acid was increased.					
37207756	7	17	theme	potential	1304:1312	arg1	risk					1314:1317	the potential risk	1300:1317	the potential risk of related complications	1300:1342	The phenotypes of the gut microbiome also changed accordingly, indicating that MGGP can inhibit the growth of pathogenic bacteria, alleviate intestinal functional metabolic disorders and reverse the potential risk of related complications.					
37207756	8	18	theme	diabetes	1453:1460	arg1	development					1438:1448	the development	1434:1448	the development of diabetes	1434:1460	Altogether, our findings demonstrate that MGGP, as a dietary polysaccharide, may inhibit the development of diabetes by reversing the imbalance of gut microbiota.					
37207756	8	18	theme	diabetes	1453:1460	arg1	polysaccharide					1406:1419	a dietary polysaccharide	1396:1419	a dietary polysaccharide	1396:1419	Altogether, our findings demonstrate that MGGP, as a dietary polysaccharide, may inhibit the development of diabetes by reversing the imbalance of gut microbiota.					
37207756	3	19	theme	antioxidant	465:475	arg1	capacity					477:484	stronger antioxidant capacity	456:484	stronger antioxidant capacity	456:484	These made MGGP have stronger antioxidant capacity and better inhibition effect on corn starch digestion in vitro.					
37207756	7	20	theme	bacteria	1226:1233	arg1	growth					1205:1210	the growth	1201:1210	the growth of pathogenic bacteria	1201:1233	The phenotypes of the gut microbiome also changed accordingly, indicating that MGGP can inhibit the growth of pathogenic bacteria, alleviate intestinal functional metabolic disorders and reverse the potential risk of related complications.					
37207756	0	21	theme	Anti-diabetic	0:12	arg1	effect					14:19	Anti-diabetic effect	0:19	Anti-diabetic effect of modified 'Guanximiyou' pummelo	0:53	Anti-diabetic effect of modified 'Guanximiyou' pummelo peel pectin on type 2 diabetic mice via gut microbiota.					
37207756	8	22	theme	dietary	1398:1404	arg1	development					1438:1448	the development	1434:1448	the development of diabetes	1434:1460	Altogether, our findings demonstrate that MGGP, as a dietary polysaccharide, may inhibit the development of diabetes by reversing the imbalance of gut microbiota.					
37207756	8	22	theme	dietary	1398:1404	arg1	polysaccharide					1406:1419	a dietary polysaccharide	1396:1419	a dietary polysaccharide	1396:1419	Altogether, our findings demonstrate that MGGP, as a dietary polysaccharide, may inhibit the development of diabetes by reversing the imbalance of gut microbiota.					
37207756	1	23	theme	nature	161:166	arg1	mechanisms					147:156	the mechanisms	143:156	the mechanisms of nature and modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP)	143:228	This study aimed to investigate the mechanisms of nature and modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP) in alleviating T2DM through in vitro and in vivo.					
37207756	0	24	theme	modified	24:31	arg1	pummelo					47:53	modified 'Guanximiyou' pummelo	24:53	modified 'Guanximiyou' pummelo	24:53	Anti-diabetic effect of modified 'Guanximiyou' pummelo peel pectin on type 2 diabetic mice via gut microbiota.					
37207756	3	25	contain	have	451:454	arg2	capacity					477:484	stronger antioxidant capacity	456:484	stronger antioxidant capacity	456:484	These made MGGP have stronger antioxidant capacity and better inhibition effect on corn starch digestion in vitro.					
37207756	3	25	contain	have	451:454	arg1	MGGP					446:449	These made MGGP	435:449	These made MGGP	435:449	These made MGGP have stronger antioxidant capacity and better inhibition effect on corn starch digestion in vitro.					
37207756	3	25	contain	have	451:454	arg2	effect					508:513	better inhibition effect	490:513	better inhibition effect	490:513	These made MGGP have stronger antioxidant capacity and better inhibition effect on corn starch digestion in vitro.					
37207756	6	26	theme	Akkermansia	1040:1050	arg1	abundance					1027:1035	the relative abundance	1014:1035	the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae	1014:1102	In addition, 16S rRNA analysis showed that MGGP changed the composition of intestinal microbiota in diabetic mice, decreased the abundance of Proteobacteria, and increased the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae.					
37207756	4	27	dep	In	550:551	arg1	vivo					553:556	vivo	553:556	vivo	553:556	In vivo experiments have shown that both GGP and MGGP inhibited the development of diabetes after 4 weeks of ingestion.					
37207756	6	28	theme	16S	855:857	arg1	analysis					864:871	16S rRNA analysis	855:871	16S rRNA analysis	855:871	In addition, 16S rRNA analysis showed that MGGP changed the composition of intestinal microbiota in diabetic mice, decreased the abundance of Proteobacteria, and increased the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae.					
37207756	0	29	theme	gut	95:97	arg1	microbiota					99:108	gut microbiota	95:108	gut microbiota	95:108	Anti-diabetic effect of modified 'Guanximiyou' pummelo peel pectin on type 2 diabetic mice via gut microbiota.					
37207756	6	30	theme	Ruminococcaceae	1088:1102	arg1	abundance					1027:1035	the relative abundance	1014:1035	the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae	1014:1102	In addition, 16S rRNA analysis showed that MGGP changed the composition of intestinal microbiota in diabetic mice, decreased the abundance of Proteobacteria, and increased the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae.					
37207756	3	31	theme	stronger	456:463	arg1	capacity					477:484	stronger antioxidant capacity	456:484	stronger antioxidant capacity	456:484	These made MGGP have stronger antioxidant capacity and better inhibition effect on corn starch digestion in vitro.					
37207756	1	32	theme	modified	172:179	arg1	pectin					208:213	modified 'Guanximiyou' pummelo peel pectin	172:213	modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP)	172:228	This study aimed to investigate the mechanisms of nature and modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP) in alleviating T2DM through in vitro and in vivo.					
37207756	0	33	theme	Guanximiyou	34:44	arg1	pummelo					47:53	modified 'Guanximiyou' pummelo	24:53	modified 'Guanximiyou' pummelo	24:53	Anti-diabetic effect of modified 'Guanximiyou' pummelo peel pectin on type 2 diabetic mice via gut microbiota.					
37207756	1	34	dep	pectin	208:213	arg1	GGP					216:218	GGP	216:218	GGP	216:218	This study aimed to investigate the mechanisms of nature and modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP) in alleviating T2DM through in vitro and in vivo.					
37207756	1	34	dep	pectin	208:213	arg1	MGGP					224:227	MGGP	224:227	MGGP	224:227	This study aimed to investigate the mechanisms of nature and modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP) in alleviating T2DM through in vitro and in vivo.					
37207756	3	35	theme	inhibition	497:506	arg1	effect					508:513	better inhibition effect	490:513	better inhibition effect	490:513	These made MGGP have stronger antioxidant capacity and better inhibition effect on corn starch digestion in vitro.					
37207756	5	36	theme	significant	765:775	arg1	capacity					789:796	significant antioxidant capacity	765:796	significant antioxidant capacity	765:796	However, MGGP can more effectively reduce blood glucose and regulate lipid metabolism, and has significant antioxidant capacity and the ability to promote SCFAs secretion.					
37207756	4	37	theme	In	550:551	arg1	experiments					558:568	In vivo experiments	550:568	In vivo experiments	550:568	In vivo experiments have shown that both GGP and MGGP inhibited the development of diabetes after 4 weeks of ingestion.					
37207756	8	38	theme	microbiota	1496:1505	arg1	imbalance					1479:1487	the imbalance	1475:1487	the imbalance of gut microbiota	1475:1505	Altogether, our findings demonstrate that MGGP, as a dietary polysaccharide, may inhibit the development of diabetes by reversing the imbalance of gut microbiota.					
37207756	0	39	from	pectin	60:65	arg1	mice					86:89	type 2 diabetic mice	70:89	type 2 diabetic mice	70:89	Anti-diabetic effect of modified 'Guanximiyou' pummelo peel pectin on type 2 diabetic mice via gut microbiota.					
37207756	2	40	theme	low	357:359	arg1	LMP					377:379	LMP	377:379	LMP	377:379	After modification, pectin was transformed from high methoxy pectin (HMP) to low methoxy pectin (LMP), and the content of galacturonic acid was increased.					
37207756	2	40	theme	low	357:359	arg1	pectin					369:374	low methoxy pectin	357:374	low methoxy pectin (LMP)	357:380	After modification, pectin was transformed from high methoxy pectin (HMP) to low methoxy pectin (LMP), and the content of galacturonic acid was increased.					
37207756	1	41	theme	Guanximiyou	182:192	arg1	pectin					208:213	modified 'Guanximiyou' pummelo peel pectin	172:213	modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP)	172:228	This study aimed to investigate the mechanisms of nature and modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP) in alleviating T2DM through in vitro and in vivo.					
37207756	0	42	theme	pummelo	47:53	arg1	effect					14:19	Anti-diabetic effect	0:19	Anti-diabetic effect of modified 'Guanximiyou' pummelo	0:53	Anti-diabetic effect of modified 'Guanximiyou' pummelo peel pectin on type 2 diabetic mice via gut microbiota.					
37207756	8	43	theme	gut	1492:1494	arg1	microbiota					1496:1505	gut microbiota	1492:1505	gut microbiota	1492:1505	Altogether, our findings demonstrate that MGGP, as a dietary polysaccharide, may inhibit the development of diabetes by reversing the imbalance of gut microbiota.					
37207756	6	44	theme	rRNA	859:862	arg1	analysis					864:871	16S rRNA analysis	855:871	16S rRNA analysis	855:871	In addition, 16S rRNA analysis showed that MGGP changed the composition of intestinal microbiota in diabetic mice, decreased the abundance of Proteobacteria, and increased the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae.					
37207756	5	45	theme	blood	712:716	arg1	glucose					718:724	blood glucose	712:724	blood glucose	712:724	However, MGGP can more effectively reduce blood glucose and regulate lipid metabolism, and has significant antioxidant capacity and the ability to promote SCFAs secretion.					
37207756	7	46	theme	complications	1330:1342	arg1	risk					1314:1317	the potential risk	1300:1317	the potential risk of related complications	1300:1342	The phenotypes of the gut microbiome also changed accordingly, indicating that MGGP can inhibit the growth of pathogenic bacteria, alleviate intestinal functional metabolic disorders and reverse the potential risk of related complications.					
37207756	3	47	theme	better	490:495	arg1	effect					508:513	better inhibition effect	490:513	better inhibition effect	490:513	These made MGGP have stronger antioxidant capacity and better inhibition effect on corn starch digestion in vitro.					
37207756	6	48	theme	relative	1018:1025	arg1	abundance					1027:1035	the relative abundance	1014:1035	the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae	1014:1102	In addition, 16S rRNA analysis showed that MGGP changed the composition of intestinal microbiota in diabetic mice, decreased the abundance of Proteobacteria, and increased the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae.					
37207756	7	49	theme	metabolic	1268:1276	arg1	disorders					1278:1286	intestinal functional metabolic disorders	1246:1286	intestinal functional metabolic disorders	1246:1286	The phenotypes of the gut microbiome also changed accordingly, indicating that MGGP can inhibit the growth of pathogenic bacteria, alleviate intestinal functional metabolic disorders and reverse the potential risk of related complications.					
37207756	3	50	theme	starch	523:528	arg1	digestion					530:538	corn starch digestion	518:538	corn starch digestion in vitro	518:547	These made MGGP have stronger antioxidant capacity and better inhibition effect on corn starch digestion in vitro.					
37207756	6	51	theme	intestinal	917:926	arg1	microbiota					928:937	intestinal microbiota	917:937	intestinal microbiota	917:937	In addition, 16S rRNA analysis showed that MGGP changed the composition of intestinal microbiota in diabetic mice, decreased the abundance of Proteobacteria, and increased the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae.					
37207756	4	52	theme	ingestion	659:667	arg1	4 weeks					648:654	4 weeks	648:654	4 weeks of ingestion	648:667	In vivo experiments have shown that both GGP and MGGP inhibited the development of diabetes after 4 weeks of ingestion.					
37207756	5	53	theme	SCFAs	825:829	arg1	secretion					831:839	SCFAs secretion	825:839	SCFAs secretion	825:839	However, MGGP can more effectively reduce blood glucose and regulate lipid metabolism, and has significant antioxidant capacity and the ability to promote SCFAs secretion.					
37207756	1	54	theme	pummelo	195:201	arg1	pectin					208:213	modified 'Guanximiyou' pummelo peel pectin	172:213	modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP)	172:228	This study aimed to investigate the mechanisms of nature and modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP) in alleviating T2DM through in vitro and in vivo.					
37207756	7	55	theme	microbiome	1131:1140	arg1	phenotypes					1109:1118	The phenotypes	1105:1118	The phenotypes of the gut microbiome	1105:1140	The phenotypes of the gut microbiome also changed accordingly, indicating that MGGP can inhibit the growth of pathogenic bacteria, alleviate intestinal functional metabolic disorders and reverse the potential risk of related complications.					
37207756	6	56	theme	microbiota	928:937	arg1	composition					902:912	the composition	898:912	the composition of intestinal microbiota in diabetic mice	898:954	In addition, 16S rRNA analysis showed that MGGP changed the composition of intestinal microbiota in diabetic mice, decreased the abundance of Proteobacteria, and increased the relative abundance of Akkermansia, Lactobacillus, Oscillospirales and Ruminococcaceae.					
37207756	7	57	theme	functional	1257:1266	arg1	disorders					1278:1286	intestinal functional metabolic disorders	1246:1286	intestinal functional metabolic disorders	1246:1286	The phenotypes of the gut microbiome also changed accordingly, indicating that MGGP can inhibit the growth of pathogenic bacteria, alleviate intestinal functional metabolic disorders and reverse the potential risk of related complications.					
37207756	5	58	theme	antioxidant	777:787	arg1	capacity					789:796	significant antioxidant capacity	765:796	significant antioxidant capacity	765:796	However, MGGP can more effectively reduce blood glucose and regulate lipid metabolism, and has significant antioxidant capacity and the ability to promote SCFAs secretion.					
37207756	7	59	theme	intestinal	1246:1255	arg1	disorders					1278:1286	intestinal functional metabolic disorders	1246:1286	intestinal functional metabolic disorders	1246:1286	The phenotypes of the gut microbiome also changed accordingly, indicating that MGGP can inhibit the growth of pathogenic bacteria, alleviate intestinal functional metabolic disorders and reverse the potential risk of related complications.					
37207756	1	60	theme	peel	203:206	arg1	pectin					208:213	modified 'Guanximiyou' pummelo peel pectin	172:213	modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP)	172:228	This study aimed to investigate the mechanisms of nature and modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP) in alleviating T2DM through in vitro and in vivo.					
37207756	3	61	theme	corn	518:521	arg1	digestion					530:538	corn starch digestion	518:538	corn starch digestion in vitro	518:547	These made MGGP have stronger antioxidant capacity and better inhibition effect on corn starch digestion in vitro.					
37207756	1	62	theme	pectin	208:213	arg1	mechanisms					147:156	the mechanisms	143:156	the mechanisms of nature and modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP)	143:228	This study aimed to investigate the mechanisms of nature and modified 'Guanximiyou' pummelo peel pectin (GGP and MGGP) in alleviating T2DM through in vitro and in vivo.					
35510685	8	0	theme	SPI	1267:1269	arg1	structure					1254:1262	the ordered structure	1242:1262	the ordered structure of SPI	1242:1269	Fourier transform infrared spectroscopy shows that CP could change the secondary structure of SPI by decreasing the α-helix and β-sheet, increasing β-rotation and irregular curl, destroying the ordered structure of SPI and increasing the polarity of the amino acids exposed to the solution.					
35510685	4	1	theme	combined	603:610	arg1	bits					612:615	combined bits	603:615	combined bits	603:615	The CP quenching of SPI is static quenching, and the number of combined bits is 1.26.					
35510685	11	2	theme	Pickering	1809:1817	arg1	emulsions					1819:1827	Pickering emulsions	1809:1827	Pickering emulsions	1809:1827	This study can provide a theoretical basis for the preparation of protein-pectin complexes and provides reference for their application in food grade gels and Pickering emulsions.					
35510685	2	3	theme	Fourier	311:317	arg1	spectroscopy					328:339	Fourier infrared spectroscopy	311:339	Fourier infrared spectroscopy	311:339	The interaction mechanism and structural changes between SPI and CP were deeply studied by fluorescence spectroscopy and Fourier infrared spectroscopy.					
35510685	8	4	dep	Fourier	1052:1058	arg1	transform					1060:1068	transform	1060:1068	transform infrared spectroscopy	1060:1090	Fourier transform infrared spectroscopy shows that CP could change the secondary structure of SPI by decreasing the α-helix and β-sheet, increasing β-rotation and irregular curl, destroying the ordered structure of SPI and increasing the polarity of the amino acids exposed to the solution.					
35510685	8	5	theme	SPI	1146:1148	arg1	structure					1133:1141	the secondary structure	1119:1141	the secondary structure of SPI	1119:1148	Fourier transform infrared spectroscopy shows that CP could change the secondary structure of SPI by decreasing the α-helix and β-sheet, increasing β-rotation and irregular curl, destroying the ordered structure of SPI and increasing the polarity of the amino acids exposed to the solution.					
35510685	11	6	theme	protein-pectin	1716:1729	arg1	complexes					1731:1739	protein-pectin complexes	1716:1739	protein-pectin complexes	1716:1739	This study can provide a theoretical basis for the preparation of protein-pectin complexes and provides reference for their application in food grade gels and Pickering emulsions.					
35510685	7	7	theme	spectra	907:913	arg1	results					865:871	The results	861:871	The results of three-dimensional fluorescence spectra	861:913	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of the amino acid residue microenvironment of SPI and changed the protein structure.					
35510685	4	8	theme	bits	612:615	arg1	1.26					620:623	1.26	620:623	1.26	620:623	The CP quenching of SPI is static quenching, and the number of combined bits is 1.26.					
35510685	4	8	theme	bits	612:615	arg1	number					593:598	the number	589:598	the number of combined bits	589:615	The CP quenching of SPI is static quenching, and the number of combined bits is 1.26.					
35510685	9	9	theme	microstructure	1347:1360	arg1	analysis					1362:1369	The microstructure analysis	1343:1369	The microstructure analysis	1343:1369	The microstructure analysis shows that SPI-CP composite system has honeycomb structure and dense pores.					
35510685	10	10	from	132.97°C	1640:1647	arg1	temperature					1606:1616	the denaturation temperature	1589:1616	the denaturation temperature of SPI from 119.73 to 132.97°C	1589:1647	From the perspective of reaction thermodynamics, it was found that the addition of CP could improve the thermal stability of SPI and increase the denaturation temperature of SPI from 119.73 to 132.97°C.					
35510685	3	11	theme	fluorescence	475:486	arg1	intensity					488:496	the endogenous fluorescence intensity	460:496	the endogenous fluorescence intensity of SPI	460:503	The results show that CP has a strong quenching effect on SPI's endogenous fluorescence, and with the addition of CP, the endogenous fluorescence intensity of SPI decreased from 13,565.2 to 6067.3.					
35510685	8	12	theme	acids	1312:1316	arg1	polarity					1290:1297	the polarity	1286:1297	the polarity of the amino acids exposed to the solution	1286:1340	Fourier transform infrared spectroscopy shows that CP could change the secondary structure of SPI by decreasing the α-helix and β-sheet, increasing β-rotation and irregular curl, destroying the ordered structure of SPI and increasing the polarity of the amino acids exposed to the solution.					
35510685	5	13	theme	three-dimensional	641:657	arg1	spectra					672:678	three-dimensional fluorescence spectra	641:678	three-dimensional fluorescence spectra	641:678	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of SPI amino acid residue microenvironment and changed the protein structure.					
35510685	4	14	theme	SPI	560:562	arg1	quenching					574:582	static quenching	567:582	static quenching	567:582	The CP quenching of SPI is static quenching, and the number of combined bits is 1.26.					
35510685	4	14	theme	SPI	560:562	arg1	quenching					547:555	The CP quenching	540:555	The CP quenching of SPI	540:562	The CP quenching of SPI is static quenching, and the number of combined bits is 1.26.					
35510685	11	15	theme	food	1789:1792	arg1	gels					1800:1803	food grade gels	1789:1803	food grade gels	1789:1803	This study can provide a theoretical basis for the preparation of protein-pectin complexes and provides reference for their application in food grade gels and Pickering emulsions.					
35510685	7	16	theme	three-dimensional	876:892	arg1	spectra					907:913	three-dimensional fluorescence spectra	876:913	three-dimensional fluorescence spectra	876:913	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of the amino acid residue microenvironment of SPI and changed the protein structure.					
35510685	9	17	theme	honeycomb	1410:1418	arg1	structure					1420:1428	honeycomb structure	1410:1428	honeycomb structure	1410:1428	The microstructure analysis shows that SPI-CP composite system has honeycomb structure and dense pores.					
35510685	5	18	theme	fluorescence	659:670	arg1	spectra					672:678	three-dimensional fluorescence spectra	641:678	three-dimensional fluorescence spectra	641:678	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of SPI amino acid residue microenvironment and changed the protein structure.					
35510685	11	19	theme	grade	1794:1798	arg1	gels					1800:1803	food grade gels	1789:1803	food grade gels	1789:1803	This study can provide a theoretical basis for the preparation of protein-pectin complexes and provides reference for their application in food grade gels and Pickering emulsions.					
35510685	5	20	theme	residue	750:756	arg1	microenvironment					758:773	SPI amino acid residue microenvironment	735:773	SPI amino acid residue microenvironment	735:773	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of SPI amino acid residue microenvironment and changed the protein structure.					
35510685	3	21	contain	has	367:369	arg1	CP					364:365	CP	364:365	CP	364:365	The results show that CP has a strong quenching effect on SPI's endogenous fluorescence, and with the addition of CP, the endogenous fluorescence intensity of SPI decreased from 13,565.2 to 6067.3.					
35510685	3	21	contain	has	367:369	arg2	effect					390:395	a strong quenching effect	371:395	a strong quenching effect on SPI's endogenous fluorescence	371:428	The results show that CP has a strong quenching effect on SPI's endogenous fluorescence, and with the addition of CP, the endogenous fluorescence intensity of SPI decreased from 13,565.2 to 6067.3.					
35510685	11	22	from	application	1774:1784	arg1	gels					1800:1803	food grade gels	1789:1803	food grade gels	1789:1803	This study can provide a theoretical basis for the preparation of protein-pectin complexes and provides reference for their application in food grade gels and Pickering emulsions.					
35510685	11	22	from	application	1774:1784	arg1	emulsions					1819:1827	Pickering emulsions	1809:1827	Pickering emulsions	1809:1827	This study can provide a theoretical basis for the preparation of protein-pectin complexes and provides reference for their application in food grade gels and Pickering emulsions.					
35510685	3	23	dep	6067.3	532:537	arg1	to					529:530	to	529:530	to	529:530	The results show that CP has a strong quenching effect on SPI's endogenous fluorescence, and with the addition of CP, the endogenous fluorescence intensity of SPI decreased from 13,565.2 to 6067.3.					
35510685	5	24	theme	CP	708:709	arg1	addition					696:703	the addition	692:703	the addition of CP	692:709	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of SPI amino acid residue microenvironment and changed the protein structure.					
35510685	5	25	theme	spectra	672:678	arg1	results					630:636	The results	626:636	The results of three-dimensional fluorescence spectra	626:678	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of SPI amino acid residue microenvironment and changed the protein structure.					
35510685	6	26	theme	Hydrophobic	810:820	arg1	interaction					822:832	Hydrophobic interaction	810:832	Hydrophobic interaction	810:832	Hydrophobic interaction exists between CP and SPI.					
35510685	7	27	theme	protein	1033:1039	arg1	structure					1041:1049	the protein structure	1029:1049	the protein structure	1029:1049	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of the amino acid residue microenvironment of SPI and changed the protein structure.					
35510685	2	28	theme	structural	220:229	arg1	changes					231:237	structural changes	220:237	structural changes between SPI and CP	220:256	The interaction mechanism and structural changes between SPI and CP were deeply studied by fluorescence spectroscopy and Fourier infrared spectroscopy.					
35510685	9	29	theme	SPI-CP	1382:1387	arg1	system					1399:1404	SPI-CP composite system	1382:1404	SPI-CP composite system	1382:1404	The microstructure analysis shows that SPI-CP composite system has honeycomb structure and dense pores.					
35510685	8	30	dep	transform	1060:1068	arg1	infrared					1070:1077	infrared	1070:1077	transform infrared spectroscopy	1060:1090	Fourier transform infrared spectroscopy shows that CP could change the secondary structure of SPI by decreasing the α-helix and β-sheet, increasing β-rotation and irregular curl, destroying the ordered structure of SPI and increasing the polarity of the amino acids exposed to the solution.					
35510685	10	31	theme	thermodynamics	1480:1493	arg1	perspective					1456:1466	the perspective	1452:1466	the perspective of reaction thermodynamics	1452:1493	From the perspective of reaction thermodynamics, it was found that the addition of CP could improve the thermal stability of SPI and increase the denaturation temperature of SPI from 119.73 to 132.97°C.					
35510685	8	32	theme	amino	1306:1310	arg1	acids					1312:1316	the amino acids	1302:1316	the amino acids exposed to the solution	1302:1340	Fourier transform infrared spectroscopy shows that CP could change the secondary structure of SPI by decreasing the α-helix and β-sheet, increasing β-rotation and irregular curl, destroying the ordered structure of SPI and increasing the polarity of the amino acids exposed to the solution.					
35510685	10	33	theme	CP	1530:1531	arg1	addition					1518:1525	the addition	1514:1525	the addition of CP	1514:1531	From the perspective of reaction thermodynamics, it was found that the addition of CP could improve the thermal stability of SPI and increase the denaturation temperature of SPI from 119.73 to 132.97°C.					
35510685	3	34	theme	strong	373:378	arg1	effect					390:395	a strong quenching effect	371:395	a strong quenching effect on SPI's endogenous fluorescence	371:428	The results show that CP has a strong quenching effect on SPI's endogenous fluorescence, and with the addition of CP, the endogenous fluorescence intensity of SPI decreased from 13,565.2 to 6067.3.					
35510685	8	35	theme	secondary	1123:1131	arg1	structure					1133:1141	the secondary structure	1119:1141	the secondary structure of SPI	1119:1148	Fourier transform infrared spectroscopy shows that CP could change the secondary structure of SPI by decreasing the α-helix and β-sheet, increasing β-rotation and irregular curl, destroying the ordered structure of SPI and increasing the polarity of the amino acids exposed to the solution.					
35510685	9	36	contain	has	1406:1408	arg1	system					1399:1404	SPI-CP composite system	1382:1404	SPI-CP composite system	1382:1404	The microstructure analysis shows that SPI-CP composite system has honeycomb structure and dense pores.					
35510685	9	36	contain	has	1406:1408	arg2	pores					1440:1444	dense pores	1434:1444	dense pores	1434:1444	The microstructure analysis shows that SPI-CP composite system has honeycomb structure and dense pores.					
35510685	9	36	contain	has	1406:1408	arg2	structure					1420:1428	honeycomb structure	1410:1428	honeycomb structure	1410:1428	The microstructure analysis shows that SPI-CP composite system has honeycomb structure and dense pores.					
35510685	3	37	theme	quenching	380:388	arg1	effect					390:395	a strong quenching effect	371:395	a strong quenching effect on SPI's endogenous fluorescence	371:428	The results show that CP has a strong quenching effect on SPI's endogenous fluorescence, and with the addition of CP, the endogenous fluorescence intensity of SPI decreased from 13,565.2 to 6067.3.					
35510685	0	38	theme	soybean	30:36	arg1	isolate					46:52	soybean protein isolate	30:52	soybean protein isolate	30:52	Interaction mechanism between soybean protein isolate and citrus pectin.					
35510685	10	39	theme	SPI	1621:1623	arg1	temperature					1606:1616	the denaturation temperature	1589:1616	the denaturation temperature of SPI from 119.73 to 132.97°C	1589:1647	From the perspective of reaction thermodynamics, it was found that the addition of CP could improve the thermal stability of SPI and increase the denaturation temperature of SPI from 119.73 to 132.97°C.					
35510685	7	40	theme	fluorescence	894:905	arg1	spectra					907:913	three-dimensional fluorescence spectra	876:913	three-dimensional fluorescence spectra	876:913	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of the amino acid residue microenvironment of SPI and changed the protein structure.					
35510685	1	41	theme	citrus	88:93	arg1	isolate					127:133	soybean protein isolate	111:133	soybean protein isolate (SPI)	111:139	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	41	theme	citrus	88:93	arg1	materials					158:166	raw materials	154:166	raw materials	154:166	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	41	theme	citrus	88:93	arg1	CP					103:104	CP	103:104	CP	103:104	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	41	theme	citrus	88:93	arg1	pectin					95:100	citrus pectin	88:100	citrus pectin (CP)	88:105	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	3	42	theme	endogenous	406:415	arg1	fluorescence					417:428	SPI's endogenous fluorescence	400:428	SPI's endogenous fluorescence	400:428	The results show that CP has a strong quenching effect on SPI's endogenous fluorescence, and with the addition of CP, the endogenous fluorescence intensity of SPI decreased from 13,565.2 to 6067.3.					
35510685	5	43	theme	amino	739:743	arg1	microenvironment					758:773	SPI amino acid residue microenvironment	735:773	SPI amino acid residue microenvironment	735:773	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of SPI amino acid residue microenvironment and changed the protein structure.					
35510685	10	44	theme	reaction	1471:1478	arg1	thermodynamics					1480:1493	reaction thermodynamics	1471:1493	reaction thermodynamics	1471:1493	From the perspective of reaction thermodynamics, it was found that the addition of CP could improve the thermal stability of SPI and increase the denaturation temperature of SPI from 119.73 to 132.97°C.					
35510685	7	45	theme	SPI	1013:1015	arg1	microenvironment					993:1008	the amino acid residue microenvironment	970:1008	the amino acid residue microenvironment of SPI	970:1015	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of the amino acid residue microenvironment of SPI and changed the protein structure.					
35510685	3	46	theme	SPI	501:503	arg1	intensity					488:496	the endogenous fluorescence intensity	460:496	the endogenous fluorescence intensity of SPI	460:503	The results show that CP has a strong quenching effect on SPI's endogenous fluorescence, and with the addition of CP, the endogenous fluorescence intensity of SPI decreased from 13,565.2 to 6067.3.					
35510685	1	47	theme	raw	154:156	arg1	isolate					127:133	soybean protein isolate	111:133	soybean protein isolate (SPI)	111:139	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	47	theme	raw	154:156	arg1	materials					158:166	raw materials	154:166	raw materials	154:166	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	47	theme	raw	154:156	arg1	pectin					95:100	citrus pectin	88:100	citrus pectin (CP)	88:105	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	11	48	theme	complexes	1731:1739	arg1	preparation					1701:1711	the preparation	1697:1711	the preparation of protein-pectin complexes	1697:1739	This study can provide a theoretical basis for the preparation of protein-pectin complexes and provides reference for their application in food grade gels and Pickering emulsions.					
35510685	7	49	theme	residue	985:991	arg1	microenvironment					993:1008	the amino acid residue microenvironment	970:1008	the amino acid residue microenvironment of SPI	970:1015	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of the amino acid residue microenvironment of SPI and changed the protein structure.					
35510685	10	50	theme	SPI	1572:1574	arg1	stability					1559:1567	the thermal stability	1547:1567	the thermal stability of SPI	1547:1574	From the perspective of reaction thermodynamics, it was found that the addition of CP could improve the thermal stability of SPI and increase the denaturation temperature of SPI from 119.73 to 132.97°C.					
35510685	5	51	theme	microenvironment	758:773	arg1	polarity					723:730	the polarity	719:730	the polarity of SPI amino acid residue microenvironment	719:773	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of SPI amino acid residue microenvironment and changed the protein structure.					
35510685	0	52	theme	protein	38:44	arg1	isolate					46:52	soybean protein isolate	30:52	soybean protein isolate	30:52	Interaction mechanism between soybean protein isolate and citrus pectin.					
35510685	5	53	theme	protein	791:797	arg1	structure					799:807	the protein structure	787:807	the protein structure	787:807	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of SPI amino acid residue microenvironment and changed the protein structure.					
35510685	5	54	theme	SPI	735:737	arg1	microenvironment					758:773	SPI amino acid residue microenvironment	735:773	SPI amino acid residue microenvironment	735:773	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of SPI amino acid residue microenvironment and changed the protein structure.					
35510685	4	55	theme	static	567:572	arg1	quenching					574:582	static quenching	567:582	static quenching	567:582	The CP quenching of SPI is static quenching, and the number of combined bits is 1.26.					
35510685	4	55	theme	static	567:572	arg1	quenching					547:555	The CP quenching	540:555	The CP quenching of SPI	540:562	The CP quenching of SPI is static quenching, and the number of combined bits is 1.26.					
35510685	0	56	theme	citrus	58:63	arg1	pectin					65:70	citrus pectin	58:70	citrus pectin	58:70	Interaction mechanism between soybean protein isolate and citrus pectin.					
35510685	7	57	theme	amino	974:978	arg1	residue					985:991	amino acid residue	974:991	the amino acid residue microenvironment of SPI	970:1015	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of the amino acid residue microenvironment of SPI and changed the protein structure.					
35510685	9	58	theme	dense	1434:1438	arg1	pores					1440:1444	dense pores	1434:1444	dense pores	1434:1444	The microstructure analysis shows that SPI-CP composite system has honeycomb structure and dense pores.					
35510685	7	59	theme	microenvironment	993:1008	arg1	polarity					958:965	the polarity	954:965	the polarity of the amino acid residue microenvironment of SPI	954:1015	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of the amino acid residue microenvironment of SPI and changed the protein structure.					
35510685	7	60	theme	acid	980:983	arg1	residue					985:991	amino acid residue	974:991	the amino acid residue microenvironment of SPI	970:1015	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of the amino acid residue microenvironment of SPI and changed the protein structure.					
35510685	7	61	theme	CP	943:944	arg1	addition					931:938	the addition	927:938	the addition of CP	927:944	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of the amino acid residue microenvironment of SPI and changed the protein structure.					
35510685	2	62	theme	fluorescence	281:292	arg1	spectroscopy					294:305	fluorescence spectroscopy	281:305	fluorescence spectroscopy	281:305	The interaction mechanism and structural changes between SPI and CP were deeply studied by fluorescence spectroscopy and Fourier infrared spectroscopy.					
35510685	1	63	theme	soybean	111:117	arg1	materials					158:166	raw materials	154:166	raw materials	154:166	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	63	theme	soybean	111:117	arg1	SPI					136:138	SPI	136:138	SPI	136:138	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	63	theme	soybean	111:117	arg1	isolate					127:133	soybean protein isolate	111:133	soybean protein isolate (SPI)	111:139	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	63	theme	soybean	111:117	arg1	pectin					95:100	citrus pectin	88:100	citrus pectin (CP)	88:105	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	64	used	used	146:149	arg2	pectin					95:100	citrus pectin	88:100	citrus pectin (CP)	88:105	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	64	used	used	146:149	arg2	materials					158:166	raw materials	154:166	raw materials	154:166	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	64	used	used	146:149	arg2	isolate					127:133	soybean protein isolate	111:133	soybean protein isolate (SPI)	111:139	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	64	used	used	146:149	arg2	CP					103:104	CP	103:104	CP	103:104	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	64	used	used	146:149	arg2	SPI					136:138	SPI	136:138	SPI	136:138	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	8	65	theme	ordered	1246:1252	arg1	structure					1254:1262	the ordered structure	1242:1262	the ordered structure of SPI	1242:1269	Fourier transform infrared spectroscopy shows that CP could change the secondary structure of SPI by decreasing the α-helix and β-sheet, increasing β-rotation and irregular curl, destroying the ordered structure of SPI and increasing the polarity of the amino acids exposed to the solution.					
35510685	10	66	dep	132.97°C	1640:1647	arg1	to					1637:1638	to	1637:1638	to	1637:1638	From the perspective of reaction thermodynamics, it was found that the addition of CP could improve the thermal stability of SPI and increase the denaturation temperature of SPI from 119.73 to 132.97°C.					
35510685	4	67	theme	CP	544:545	arg1	quenching					574:582	static quenching	567:582	static quenching	567:582	The CP quenching of SPI is static quenching, and the number of combined bits is 1.26.					
35510685	4	67	theme	CP	544:545	arg1	quenching					547:555	The CP quenching	540:555	The CP quenching of SPI	540:562	The CP quenching of SPI is static quenching, and the number of combined bits is 1.26.					
35510685	3	68	theme	endogenous	464:473	arg1	intensity					488:496	the endogenous fluorescence intensity	460:496	the endogenous fluorescence intensity of SPI	460:503	The results show that CP has a strong quenching effect on SPI's endogenous fluorescence, and with the addition of CP, the endogenous fluorescence intensity of SPI decreased from 13,565.2 to 6067.3.					
35510685	10	69	theme	denaturation	1593:1604	arg1	temperature					1606:1616	the denaturation temperature	1589:1616	the denaturation temperature of SPI from 119.73 to 132.97°C	1589:1647	From the perspective of reaction thermodynamics, it was found that the addition of CP could improve the thermal stability of SPI and increase the denaturation temperature of SPI from 119.73 to 132.97°C.					
35510685	1	70	theme	protein	119:125	arg1	materials					158:166	raw materials	154:166	raw materials	154:166	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	70	theme	protein	119:125	arg1	SPI					136:138	SPI	136:138	SPI	136:138	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	70	theme	protein	119:125	arg1	isolate					127:133	soybean protein isolate	111:133	soybean protein isolate (SPI)	111:139	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	1	70	theme	protein	119:125	arg1	pectin					95:100	citrus pectin	88:100	citrus pectin (CP)	88:105	In this study, citrus pectin (CP) and soybean protein isolate (SPI) were used as raw materials to prepare a complex.					
35510685	8	71	theme	irregular	1215:1223	arg1	curl					1225:1228	irregular curl	1215:1228	irregular curl	1215:1228	Fourier transform infrared spectroscopy shows that CP could change the secondary structure of SPI by decreasing the α-helix and β-sheet, increasing β-rotation and irregular curl, destroying the ordered structure of SPI and increasing the polarity of the amino acids exposed to the solution.					
35510685	2	72	theme	interaction	194:204	arg1	mechanism					206:214	The interaction mechanism	190:214	The interaction mechanism	190:214	The interaction mechanism and structural changes between SPI and CP were deeply studied by fluorescence spectroscopy and Fourier infrared spectroscopy.					
35510685	5	73	theme	acid	745:748	arg1	microenvironment					758:773	SPI amino acid residue microenvironment	735:773	SPI amino acid residue microenvironment	735:773	The results of three-dimensional fluorescence spectra showed that the addition of CP reduced the polarity of SPI amino acid residue microenvironment and changed the protein structure.					
35510685	3	74	theme	CP	456:457	arg1	addition					444:451	the addition	440:451	the addition of CP	440:457	The results show that CP has a strong quenching effect on SPI's endogenous fluorescence, and with the addition of CP, the endogenous fluorescence intensity of SPI decreased from 13,565.2 to 6067.3.					
35510685	11	75	theme	theoretical	1675:1685	arg1	basis					1687:1691	a theoretical basis	1673:1691	a theoretical basis for the preparation of protein-pectin complexes	1673:1739	This study can provide a theoretical basis for the preparation of protein-pectin complexes and provides reference for their application in food grade gels and Pickering emulsions.					
35510685	10	76	theme	thermal	1551:1557	arg1	stability					1559:1567	the thermal stability	1547:1567	the thermal stability of SPI	1547:1574	From the perspective of reaction thermodynamics, it was found that the addition of CP could improve the thermal stability of SPI and increase the denaturation temperature of SPI from 119.73 to 132.97°C.					
35510685	9	77	theme	composite	1389:1397	arg1	system					1399:1404	SPI-CP composite system	1382:1404	SPI-CP composite system	1382:1404	The microstructure analysis shows that SPI-CP composite system has honeycomb structure and dense pores.					
35510685	3	78	from	effect	390:395	arg1	fluorescence					417:428	SPI's endogenous fluorescence	400:428	SPI's endogenous fluorescence	400:428	The results show that CP has a strong quenching effect on SPI's endogenous fluorescence, and with the addition of CP, the endogenous fluorescence intensity of SPI decreased from 13,565.2 to 6067.3.					
35510685	2	79	theme	infrared	319:326	arg1	spectroscopy					328:339	Fourier infrared spectroscopy	311:339	Fourier infrared spectroscopy	311:339	The interaction mechanism and structural changes between SPI and CP were deeply studied by fluorescence spectroscopy and Fourier infrared spectroscopy.					
35351304	3	0	theme	PbWoxT1	706:712	arg1	mRNA					714:717	PbWoxT1 mRNA	706:717	PbWoxT1 mRNA	706:717	We identified an ankyrin protein, PbANK, that interacts with PbPTB3 to facilitate its transport through the phloem alongside PbWoxT1 mRNA.					
35351304	7	1	theme	PbWoxT1	1263:1269	arg1	movement					1240:1247	the long-distance movement	1222:1247	the long-distance movement of PbPTB3 and PbWoxT1	1222:1269	Collectively, these findings demonstrate that PbANK mediates the long-distance movement of PbPTB3 and PbWoxT1 by degrading callose to increase the efficiency of cell-to-cell movement.					
35351304	2	2	used	used	448:451	arg2	we					445:446	we	445:446	we	445:446	Here, we used the core of PbPTB3-PbWoxT1 RNP complex, PbPTB3, as bait to screen Pyrus betulaefolia cDNA library for its interaction partners.					
35351304	2	3	theme	interaction	559:569	arg1	partners					571:578	its interaction partners	555:578	its interaction partners	555:578	Here, we used the core of PbPTB3-PbWoxT1 RNP complex, PbPTB3, as bait to screen Pyrus betulaefolia cDNA library for its interaction partners.					
35351304	1	4	from	changes	165:171	arg1	materials					188:196	the grafted materials	176:196	the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union	176:295	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	2	5	dep	Pyrus	519:523	arg1	betulaefolia					525:536	betulaefolia	525:536	betulaefolia	525:536	Here, we used the core of PbPTB3-PbWoxT1 RNP complex, PbPTB3, as bait to screen Pyrus betulaefolia cDNA library for its interaction partners.					
35351304	0	6	theme	deposited	92:100	arg1	callose					102:108	deposited callose	92:108	deposited callose	92:108	PbANK facilitates the long-distance movement of the PbWoxT1-PbPTB3 RNP complex by degrading deposited callose.					
35351304	4	7	theme	Heterografting	720:733	arg1	experiments					735:745	Heterografting experiments	720:745	Heterografting experiments	720:745	Heterografting experiments showed that silencing PbANK in rootstock prevented the transport of PbPTB3 and PbWoxT1 mRNA from the rootstock to the scion.					
35351304	6	8	theme	intercellular	1076:1088	arg1	diffusion					1090:1098	the intercellular diffusion	1072:1098	the intercellular diffusion of PbPTB3	1072:1108	Fluorescence microscopy showed that silencing ANK affected the intercellular diffusion of PbPTB3 and increased callose deposition at plasmodesmata.					
35351304	2	9	theme	complex	484:490	arg1	core					457:460	the core	453:460	the core of PbPTB3-PbWoxT1 RNP complex, PbPTB3,	453:499	Here, we used the core of PbPTB3-PbWoxT1 RNP complex, PbPTB3, as bait to screen Pyrus betulaefolia cDNA library for its interaction partners.					
35351304	1	10	theme	trafficking	348:358	arg1	complexes					384:392	trafficking ribonucleoprotein (RNP) complexes	348:392	trafficking ribonucleoprotein (RNP) complexes	348:392	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	4	11	theme	PbPTB3	815:820	arg1	transport					802:810	the transport	798:810	the transport of PbPTB3 and PbWoxT1 mRNA	798:837	Heterografting experiments showed that silencing PbANK in rootstock prevented the transport of PbPTB3 and PbWoxT1 mRNA from the rootstock to the scion.					
35351304	1	12	theme	grafted	180:186	arg1	materials					188:196	the grafted materials	176:196	the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union	176:295	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	1	13	theme	ribonucleoprotein	360:376	arg1	complexes					384:392	trafficking ribonucleoprotein (RNP) complexes	348:392	trafficking ribonucleoprotein (RNP) complexes	348:392	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	4	14	theme	mRNA	834:837	arg1	transport					802:810	the transport	798:810	the transport of PbPTB3 and PbWoxT1 mRNA	798:837	Heterografting experiments showed that silencing PbANK in rootstock prevented the transport of PbPTB3 and PbWoxT1 mRNA from the rootstock to the scion.					
35351304	0	15	theme	complex	71:77	arg1	movement					36:43	the long-distance movement	18:43	the long-distance movement of the PbWoxT1-PbPTB3 RNP complex	18:77	PbANK facilitates the long-distance movement of the PbWoxT1-PbPTB3 RNP complex by degrading deposited callose.					
35351304	4	16	theme	PbWoxT1	826:832	arg1	mRNA					834:837	PbWoxT1 mRNA	826:837	PbWoxT1 mRNA	826:837	Heterografting experiments showed that silencing PbANK in rootstock prevented the transport of PbPTB3 and PbWoxT1 mRNA from the rootstock to the scion.					
35351304	1	17	theme	due	198:200	arg1	materials					188:196	the grafted materials	176:196	the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union	176:295	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	0	18	theme	long-distance	22:34	arg1	movement					36:43	the long-distance movement	18:43	the long-distance movement of the PbWoxT1-PbPTB3 RNP complex	18:77	PbANK facilitates the long-distance movement of the PbWoxT1-PbPTB3 RNP complex by degrading deposited callose.					
35351304	6	19	theme	silencing	1049:1057	arg1	ANK					1059:1061	silencing ANK	1049:1061	silencing ANK	1049:1061	Fluorescence microscopy showed that silencing ANK affected the intercellular diffusion of PbPTB3 and increased callose deposition at plasmodesmata.					
35351304	1	20	theme	graft	285:289	arg1	union					291:295	the graft union	281:295	the graft union	281:295	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	1	21	theme	Grafting	111:118	arg1	crops					134:138	Grafting horticultural crops	111:138	Grafting horticultural crops	111:138	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	0	22	theme	RNP	67:69	arg1	complex					71:77	the PbWoxT1-PbPTB3 RNP complex	48:77	the PbWoxT1-PbPTB3 RNP complex	48:77	PbANK facilitates the long-distance movement of the PbWoxT1-PbPTB3 RNP complex by degrading deposited callose.					
35351304	1	23	theme	RNP	379:381	arg1	complexes					384:392	trafficking ribonucleoprotein (RNP) complexes	348:392	trafficking ribonucleoprotein (RNP) complexes	348:392	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	5	24	theme	graft	1000:1004	arg1	union					1006:1010	a graft union	998:1010	a graft union	998:1010	Similarly, heterologous grafting experiments demonstrated that PbANK itself cannot be transported over long distances through a graft union.					
35351304	6	25	theme	Fluorescence	1013:1024	arg1	microscopy					1026:1035	Fluorescence microscopy	1013:1035	Fluorescence microscopy	1013:1035	Fluorescence microscopy showed that silencing ANK affected the intercellular diffusion of PbPTB3 and increased callose deposition at plasmodesmata.					
35351304	3	26	theme	ankyrin	598:604	arg1	PbANK					615:619	PbANK	615:619	PbANK	615:619	We identified an ankyrin protein, PbANK, that interacts with PbPTB3 to facilitate its transport through the phloem alongside PbWoxT1 mRNA.					
35351304	3	26	theme	ankyrin	598:604	arg1	protein					606:612	an ankyrin protein	595:612	an ankyrin protein	595:612	We identified an ankyrin protein, PbANK, that interacts with PbPTB3 to facilitate its transport through the phloem alongside PbWoxT1 mRNA.					
35351304	7	27	theme	cell-to-cell	1322:1333	arg1	movement					1335:1342	cell-to-cell movement	1322:1342	cell-to-cell movement	1322:1342	Collectively, these findings demonstrate that PbANK mediates the long-distance movement of PbPTB3 and PbWoxT1 by degrading callose to increase the efficiency of cell-to-cell movement.					
35351304	0	28	theme	PbWoxT1-PbPTB3	52:65	arg1	complex					71:77	the PbWoxT1-PbPTB3 RNP complex	48:77	the PbWoxT1-PbPTB3 RNP complex	48:77	PbANK facilitates the long-distance movement of the PbWoxT1-PbPTB3 RNP complex by degrading deposited callose.					
35351304	7	29	theme	PbPTB3	1252:1257	arg1	movement					1240:1247	the long-distance movement	1222:1247	the long-distance movement of PbPTB3 and PbWoxT1	1222:1269	Collectively, these findings demonstrate that PbANK mediates the long-distance movement of PbPTB3 and PbWoxT1 by degrading callose to increase the efficiency of cell-to-cell movement.					
35351304	1	30	theme	complexes	384:392	arg1	composition					333:343	the composition	329:343	the composition of trafficking ribonucleoprotein (RNP) complexes	329:392	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	6	31	theme	callose	1124:1130	arg1	deposition					1132:1141	callose deposition	1124:1141	callose deposition	1124:1141	Fluorescence microscopy showed that silencing ANK affected the intercellular diffusion of PbPTB3 and increased callose deposition at plasmodesmata.					
35351304	1	32	theme	horticultural	120:132	arg1	crops					134:138	Grafting horticultural crops	111:138	Grafting horticultural crops	111:138	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	2	33	theme	cDNA	538:541	arg1	library					543:549	Pyrus betulaefolia cDNA library	519:549	Pyrus betulaefolia cDNA library	519:549	Here, we used the core of PbPTB3-PbWoxT1 RNP complex, PbPTB3, as bait to screen Pyrus betulaefolia cDNA library for its interaction partners.					
35351304	5	34	theme	heterologous	883:894	arg1	experiments					905:915	heterologous grafting experiments	883:915	heterologous grafting experiments	883:915	Similarly, heterologous grafting experiments demonstrated that PbANK itself cannot be transported over long distances through a graft union.					
35351304	5	35	theme	long	975:978	arg1	distances					980:988	long distances	975:988	long distances	975:988	Similarly, heterologous grafting experiments demonstrated that PbANK itself cannot be transported over long distances through a graft union.					
35351304	5	36	theme	grafting	896:903	arg1	experiments					905:915	heterologous grafting experiments	883:915	heterologous grafting experiments	883:915	Similarly, heterologous grafting experiments demonstrated that PbANK itself cannot be transported over long distances through a graft union.					
35351304	1	37	theme	macromolecular	221:234	arg1	proteins					264:271	proteins	264:271	proteins	264:271	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	1	37	theme	macromolecular	221:234	arg1	signals					236:242	macromolecular signals	221:242	macromolecular signals	221:242	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	1	37	theme	macromolecular	221:234	arg1	RNAs					255:258	RNAs	255:258	RNAs	255:258	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	2	38	theme	Pyrus	519:523	arg1	library					543:549	Pyrus betulaefolia cDNA library	519:549	Pyrus betulaefolia cDNA library	519:549	Here, we used the core of PbPTB3-PbWoxT1 RNP complex, PbPTB3, as bait to screen Pyrus betulaefolia cDNA library for its interaction partners.					
35351304	4	39	theme	silencing	759:767	arg1	PbANK					769:773	silencing PbANK	759:773	silencing PbANK in rootstock	759:786	Heterografting experiments showed that silencing PbANK in rootstock prevented the transport of PbPTB3 and PbWoxT1 mRNA from the rootstock to the scion.					
35351304	6	40	theme	PbPTB3	1103:1108	arg1	diffusion					1090:1098	the intercellular diffusion	1072:1098	the intercellular diffusion of PbPTB3	1072:1108	Fluorescence microscopy showed that silencing ANK affected the intercellular diffusion of PbPTB3 and increased callose deposition at plasmodesmata.					
35351304	1	41	theme	signals	236:242	arg1	movement					209:216	the movement	205:216	the movement of macromolecular signals, including RNAs and proteins, across the graft union	205:295	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	2	42	theme	RNP	480:482	arg1	PbPTB3					493:498	PbPTB3	493:498	PbPTB3	493:498	Here, we used the core of PbPTB3-PbWoxT1 RNP complex, PbPTB3, as bait to screen Pyrus betulaefolia cDNA library for its interaction partners.					
35351304	2	42	theme	RNP	480:482	arg1	complex					484:490	PbPTB3-PbWoxT1 RNP complex	465:490	PbPTB3-PbWoxT1 RNP complex	465:490	Here, we used the core of PbPTB3-PbWoxT1 RNP complex, PbPTB3, as bait to screen Pyrus betulaefolia cDNA library for its interaction partners.					
35351304	7	43	theme	movement	1335:1342	arg1	efficiency					1308:1317	the efficiency	1304:1317	the efficiency of cell-to-cell movement	1304:1342	Collectively, these findings demonstrate that PbANK mediates the long-distance movement of PbPTB3 and PbWoxT1 by degrading callose to increase the efficiency of cell-to-cell movement.					
35351304	2	44	theme	PbPTB3-PbWoxT1	465:478	arg1	PbPTB3					493:498	PbPTB3	493:498	PbPTB3	493:498	Here, we used the core of PbPTB3-PbWoxT1 RNP complex, PbPTB3, as bait to screen Pyrus betulaefolia cDNA library for its interaction partners.					
35351304	2	44	theme	PbPTB3-PbWoxT1	465:478	arg1	complex					484:490	PbPTB3-PbWoxT1 RNP complex	465:490	PbPTB3-PbWoxT1 RNP complex	465:490	Here, we used the core of PbPTB3-PbWoxT1 RNP complex, PbPTB3, as bait to screen Pyrus betulaefolia cDNA library for its interaction partners.					
35351304	4	45	from	PbANK	769:773	arg1	rootstock					778:786	rootstock	778:786	rootstock	778:786	Heterografting experiments showed that silencing PbANK in rootstock prevented the transport of PbPTB3 and PbWoxT1 mRNA from the rootstock to the scion.					
35351304	1	46	theme	phenotypic	154:163	arg1	changes					165:171	phenotypic changes	154:171	phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union	154:295	Grafting horticultural crops can result in phenotypic changes in the grafted materials due to the movement of macromolecular signals, including RNAs and proteins, across the graft union; however, little is known about the composition of trafficking ribonucleoprotein (RNP) complexes or how these macromolecules are transported.					
35351304	7	47	theme	long-distance	1226:1238	arg1	movement					1240:1247	the long-distance movement	1222:1247	the long-distance movement of PbPTB3 and PbWoxT1	1222:1269	Collectively, these findings demonstrate that PbANK mediates the long-distance movement of PbPTB3 and PbWoxT1 by degrading callose to increase the efficiency of cell-to-cell movement.					
35753678	6	0	theme	osteogenic	1309:1318	arg1	property					1320:1327	osteogenic property	1309:1327	osteogenic property	1309:1327	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	2	1	theme	pearl	257:261	arg1	biomineralization					263:279	pearl biomineralization	257:279	pearl biomineralization	257:279	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	7	2	theme	new	1528:1530	arg1	insights					1532:1539	valuable new insights	1519:1539	valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization	1519:1621	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	6	3	theme	matrix	1189:1194	arg1	proteins					1196:1203	pearl matrix proteins	1183:1203	pearl matrix proteins	1183:1203	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	2	4	theme	protein	246:252	arg1	basis					230:234	the molecular basis	216:234	the molecular basis of matrix protein in pearl biomineralization and biomedical applications	216:307	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	2	4	theme	protein	246:252	arg1	unknown					321:327	unknown	321:327	unknown	321:327	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	5	5	theme	high	869:872	arg1	frequency					874:882	the high frequency	865:882	the high frequency	865:882	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	6	6	theme	medical	1266:1272	arg1	property					1474:1481	other immunomodulatory property	1451:1481	other immunomodulatory property	1451:1481	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	6	theme	medical	1266:1272	arg1	effects					1369:1375	neuro-regulation effects	1352:1375	neuro-regulation effects	1352:1375	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	6	theme	medical	1266:1272	arg1	functions					1274:1282	their potential medical functions	1250:1282	their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property	1250:1481	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	6	theme	medical	1266:1272	arg1	activity					1342:1349	antioxidant activity	1330:1349	antioxidant activity	1330:1349	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	6	theme	medical	1266:1272	arg1	healing					1300:1306	wound healing	1294:1306	wound healing	1294:1306	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	6	theme	medical	1266:1272	arg1	property					1320:1327	osteogenic property	1309:1327	osteogenic property	1309:1327	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	3	7	from	powder	479:484	arg1	proteins					364:371	the matrix proteins	353:371	the matrix proteins of water-soluble matrix	353:395	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	3	7	from	powder	479:484	arg1	matrix					411:416	acid-soluble matrix	398:416	acid-soluble matrix	398:416	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	3	7	from	powder	479:484	arg1	matrix					437:442	acid-insoluble matrix	422:442	acid-insoluble matrix	422:442	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	8	8	theme	following	1803:1811	arg1	points					1813:1818	the following points	1799:1818	the following points	1799:1818	SIGNIFICANCE: The significance of this study included the following points.					
35753678	1	9	theme	Chinese	183:189	arg1	TCM					201:203	TCM	201:203	TCM	201:203	The freshwater pearl is one kind of valuable organic jewelry and traditional Chinese medicine (TCM).					
35753678	1	9	theme	Chinese	183:189	arg1	medicine					191:198	traditional Chinese medicine	171:198	traditional Chinese medicine (TCM)	171:204	The freshwater pearl is one kind of valuable organic jewelry and traditional Chinese medicine (TCM).					
35753678	5	10	theme	macromolecules	1070:1083	arg1	model					1086:1090	the "chitin-silk fibroin gel proteins-acidic macromolecules" model	1025:1090	the "chitin-silk fibroin gel proteins-acidic macromolecules" model	1025:1090	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	5	11	theme	matrix	845:850	arg1	proteins					852:859	pearl matrix proteins	839:859	pearl matrix proteins	839:859	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	7	12	theme	diverse	1695:1701	arg1	properties					1714:1723	their diverse biological properties	1689:1723	their diverse biological properties in TCM application	1689:1742	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	0	13	from	biomineralization	61:77	arg1	Insights					47:54	Insights	47:54	The proteomics of the freshwater pearl powder: Insights from biomineralization to biomedical application.	0:104	The proteomics of the freshwater pearl powder: Insights from biomineralization to biomedical application.					
35753678	6	14	theme	potential	1256:1264	arg1	property					1474:1481	other immunomodulatory property	1451:1481	other immunomodulatory property	1451:1481	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	14	theme	potential	1256:1264	arg1	effects					1369:1375	neuro-regulation effects	1352:1375	neuro-regulation effects	1352:1375	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	14	theme	potential	1256:1264	arg1	functions					1274:1282	their potential medical functions	1250:1282	their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property	1250:1481	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	14	theme	potential	1256:1264	arg1	activity					1342:1349	antioxidant activity	1330:1349	antioxidant activity	1330:1349	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	14	theme	potential	1256:1264	arg1	healing					1300:1306	wound healing	1294:1306	wound healing	1294:1306	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	14	theme	potential	1256:1264	arg1	property					1320:1327	osteogenic property	1309:1327	osteogenic property	1309:1327	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	15	theme	neuro-regulation	1352:1367	arg1	effects					1369:1375	neuro-regulation effects	1352:1375	neuro-regulation effects	1352:1375	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	15	theme	neuro-regulation	1352:1367	arg1	effects					1439:1445	anti-inflammatory and anti-apoptotic effects	1402:1445	anti-inflammatory and anti-apoptotic effects	1402:1445	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	15	theme	neuro-regulation	1352:1367	arg1	effect					1394:1399	skin lightening effect	1378:1399	skin lightening effect	1378:1399	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	3	16	theme	transcriptomic	605:618	arg1	database					620:627	the transcriptomic database	601:627	the transcriptomic database of the pearl sac	601:644	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	5	17	theme	chitin-silk	1030:1040	arg1	macromolecules					1070:1083	the "chitin-silk fibroin gel proteins-acidic macromolecules	1025:1083	the "chitin-silk fibroin gel proteins-acidic macromolecules" model	1025:1090	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	5	18	theme	von	927:929	arg1	factor					942:947	von Willebrand factor	927:947	von Willebrand factor type A	927:954	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	7	19	theme	proteins	1664:1671	arg1	basis					1642:1646	the molecular basis	1628:1646	the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application	1628:1742	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	5	20	theme	gel	1050:1052	arg1	macromolecules					1070:1083	the "chitin-silk fibroin gel proteins-acidic macromolecules	1025:1083	the "chitin-silk fibroin gel proteins-acidic macromolecules" model	1025:1090	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	3	21	theme	pearl	636:640	arg1	sac					642:644	the pearl sac	632:644	the pearl sac	632:644	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	5	22	theme	factor	942:947	arg1	domains					894:900	domains	894:900	domains like carbonic anhydrase	894:924	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	5	22	theme	factor	942:947	arg1	A					954:954	von Willebrand factor type A	927:954	von Willebrand factor type A	927:954	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	2	23	from	biomineralization	263:279	arg1	basis					230:234	the molecular basis	216:234	the molecular basis of matrix protein in pearl biomineralization and biomedical applications	216:307	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	2	23	from	biomineralization	263:279	arg1	unknown					321:327	unknown	321:327	unknown	321:327	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	7	24	theme	pearl	1651:1655	arg1	proteins					1664:1671	pearl matrix proteins	1651:1671	pearl matrix proteins responsible for their diverse biological properties in TCM application	1651:1742	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	5	25	theme	pearl	1125:1129	arg1	process					1149:1155	the pearl biomineralization process	1121:1155	description the pearl biomineralization process	1109:1155	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	2	26	theme	molecular	220:228	arg1	basis					230:234	the molecular basis	216:234	the molecular basis of matrix protein in pearl biomineralization and biomedical applications	216:307	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	2	26	theme	molecular	220:228	arg1	unknown					321:327	unknown	321:327	unknown	321:327	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	1	27	theme	organic	151:157	arg1	jewelry					159:165	valuable organic jewelry	142:165	valuable organic jewelry	142:165	The freshwater pearl is one kind of valuable organic jewelry and traditional Chinese medicine (TCM).					
35753678	0	28	theme	biomedical	82:91	arg1	application					93:103	biomedical application	82:103	biomedical application	82:103	The proteomics of the freshwater pearl powder: Insights from biomineralization to biomedical application.					
35753678	6	29	theme	proteins	1196:1203	arg1	ninety-one					1169:1178	ninety-one	1169:1178	ninety-one of pearl matrix proteins	1169:1203	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	29	theme	proteins	1196:1203	arg1	Meanwhile					1158:1166	Meanwhile	1158:1166	Meanwhile	1158:1166	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	5	30	theme	chitin	972:977	arg1	domains					894:900	domains	894:900	domains like carbonic anhydrase	894:924	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	5	30	theme	chitin	972:977	arg1	binding					979:985	chitin binding 2	972:987	chitin binding 2	972:987	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	6	31	theme	lightening	1383:1392	arg1	effects					1369:1375	neuro-regulation effects	1352:1375	neuro-regulation effects	1352:1375	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	31	theme	lightening	1383:1392	arg1	effect					1394:1399	skin lightening effect	1378:1399	skin lightening effect	1378:1399	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	3	32	theme	matrix	357:362	arg1	proteins					364:371	the matrix proteins	353:371	the matrix proteins of water-soluble matrix	353:395	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	3	33	theme	acid-soluble	398:409	arg1	matrix					411:416	acid-soluble matrix	398:416	acid-soluble matrix	398:416	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	7	34	from	properties	1714:1723	arg1	application					1732:1742	TCM application	1728:1742	TCM application	1728:1742	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	2	35	from	basis	230:234	arg1	biomineralization					263:279	pearl biomineralization	257:279	pearl biomineralization	257:279	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	2	35	from	basis	230:234	arg1	applications					296:307	biomedical applications	285:307	biomedical applications	285:307	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	3	36	theme	matrix	390:395	arg1	proteins					364:371	the matrix proteins	353:371	the matrix proteins of water-soluble matrix	353:395	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	3	36	theme	matrix	390:395	arg1	matrix					411:416	acid-soluble matrix	398:416	acid-soluble matrix	398:416	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	3	36	theme	matrix	390:395	arg1	matrix					437:442	acid-insoluble matrix	422:442	acid-insoluble matrix	422:442	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	0	37	theme	freshwater	22:31	arg1	powder					39:44	the freshwater pearl powder	18:44	the freshwater pearl powder	18:44	The proteomics of the freshwater pearl powder: Insights from biomineralization to biomedical application.					
35753678	7	38	theme	matrix	1578:1583	arg1	protein					1585:1591	pearl matrix protein	1572:1591	pearl matrix protein for mollusc biomineralization	1572:1621	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	6	39	theme	other	1451:1455	arg1	property					1474:1481	other immunomodulatory property	1451:1481	other immunomodulatory property	1451:1481	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	4	40	theme	potential	781:789	arg1	functions					809:817	their potential biomineralization functions	775:817	their potential biomineralization functions	775:817	The results showed that a total of 190 proteins were identified in pearl proteomics, which was divided into eight categories by their potential biomineralization functions.					
35753678	3	41	theme	seedless	464:471	arg1	powder					479:484	the freshwater seedless pearl powder	449:484	the freshwater seedless pearl powder	449:484	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	5	42	theme	protein	992:998	arg1	sequences					1000:1008	protein sequences	992:1008	protein sequences	992:1008	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	3	43	theme	liquid	506:511	arg1	LC-MS/MS					554:561	LC-MS/MS	554:561	LC-MS/MS	554:561	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	3	43	theme	liquid	506:511	arg1	spectrometry					540:551	liquid chromatography-tandem mass spectrometry	506:551	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	506:562	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	7	44	theme	valuable	1519:1526	arg1	insights					1532:1539	valuable new insights	1519:1539	valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization	1519:1621	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	5	45	theme	carbonic	907:914	arg1	anhydrase					916:924	carbonic anhydrase	907:924	carbonic anhydrase	907:924	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	6	46	theme	wound	1294:1298	arg1	healing					1300:1306	wound healing	1294:1306	wound healing	1294:1306	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	3	47	theme	mass	535:538	arg1	LC-MS/MS					554:561	LC-MS/MS	554:561	LC-MS/MS	554:561	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	3	47	theme	mass	535:538	arg1	spectrometry					540:551	liquid chromatography-tandem mass spectrometry	506:551	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	506:562	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	1	48	theme	jewelry	159:165	arg1	pearl					121:125	The freshwater pearl	106:125	The freshwater pearl	106:125	The freshwater pearl is one kind of valuable organic jewelry and traditional Chinese medicine (TCM).					
35753678	1	48	theme	jewelry	159:165	arg1	kind					134:137	one kind	130:137	one kind of valuable organic jewelry and traditional Chinese medicine (TCM)	130:204	The freshwater pearl is one kind of valuable organic jewelry and traditional Chinese medicine (TCM).					
35753678	0	49	theme	powder	39:44	arg1	proteomics					4:13	The proteomics	0:13	The proteomics of the freshwater pearl powder: Insights from biomineralization to biomedical application.	0:104	The proteomics of the freshwater pearl powder: Insights from biomineralization to biomedical application.					
35753678	6	50	theme	pearl	1183:1187	arg1	proteins					1196:1203	pearl matrix proteins	1183:1203	pearl matrix proteins	1183:1203	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	8	51	theme	study	1784:1788	arg1	significance					1763:1774	The significance	1759:1774	The significance of this study	1759:1788	SIGNIFICANCE: The significance of this study included the following points.					
35753678	1	52	theme	traditional	171:181	arg1	TCM					201:203	TCM	201:203	TCM	201:203	The freshwater pearl is one kind of valuable organic jewelry and traditional Chinese medicine (TCM).					
35753678	1	52	theme	traditional	171:181	arg1	medicine					191:198	traditional Chinese medicine	171:198	traditional Chinese medicine (TCM)	171:204	The freshwater pearl is one kind of valuable organic jewelry and traditional Chinese medicine (TCM).					
35753678	7	53	theme	biological	1703:1712	arg1	properties					1714:1723	their diverse biological properties	1689:1723	their diverse biological properties in TCM application	1689:1742	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	6	54	theme	skin	1378:1381	arg1	effects					1369:1375	neuro-regulation effects	1352:1375	neuro-regulation effects	1352:1375	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	54	theme	skin	1378:1381	arg1	effect					1394:1399	skin lightening effect	1378:1399	skin lightening effect	1378:1399	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	2	55	theme	biomedical	285:294	arg1	applications					296:307	biomedical applications	285:307	biomedical applications	285:307	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	1	56	theme	medicine	191:198	arg1	pearl					121:125	The freshwater pearl	106:125	The freshwater pearl	106:125	The freshwater pearl is one kind of valuable organic jewelry and traditional Chinese medicine (TCM).					
35753678	1	56	theme	medicine	191:198	arg1	kind					134:137	one kind	130:137	one kind of valuable organic jewelry and traditional Chinese medicine (TCM)	130:204	The freshwater pearl is one kind of valuable organic jewelry and traditional Chinese medicine (TCM).					
35753678	5	57	theme	"	1084:1084	arg1	model					1086:1090	the "chitin-silk fibroin gel proteins-acidic macromolecules" model	1025:1090	the "chitin-silk fibroin gel proteins-acidic macromolecules" model	1025:1090	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	5	58	theme	proteins	852:859	arg1	frequency					874:882	the high frequency	865:882	the high frequency	865:882	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	5	58	theme	proteins	852:859	arg1	composition					824:834	The composition	820:834	The composition of pearl matrix proteins	820:859	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	7	59	theme	responsible	1673:1683	arg1	proteins					1664:1671	pearl matrix proteins	1651:1671	pearl matrix proteins responsible for their diverse biological properties in TCM application	1651:1742	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	1	60	theme	freshwater	110:119	arg1	pearl					121:125	The freshwater pearl	106:125	The freshwater pearl	106:125	The freshwater pearl is one kind of valuable organic jewelry and traditional Chinese medicine (TCM).					
35753678	1	60	theme	freshwater	110:119	arg1	kind					134:137	one kind	130:137	one kind of valuable organic jewelry and traditional Chinese medicine (TCM)	130:204	The freshwater pearl is one kind of valuable organic jewelry and traditional Chinese medicine (TCM).					
35753678	7	61	theme	matrix	1657:1662	arg1	proteins					1664:1671	pearl matrix proteins	1651:1671	pearl matrix proteins responsible for their diverse biological properties in TCM application	1651:1742	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	6	62	theme	antioxidant	1330:1340	arg1	activity					1342:1349	antioxidant activity	1330:1349	antioxidant activity	1330:1349	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	5	63	theme	fibroin	1042:1048	arg1	macromolecules					1070:1083	the "chitin-silk fibroin gel proteins-acidic macromolecules	1025:1083	the "chitin-silk fibroin gel proteins-acidic macromolecules" model	1025:1090	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	2	64	theme	matrix	239:244	arg1	protein					246:252	matrix protein	239:252	matrix protein in pearl biomineralization and biomedical applications	239:307	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	3	65	located	detected	491:498	arg2	proteins					364:371	the matrix proteins	353:371	the matrix proteins of water-soluble matrix	353:395	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	3	65	located	detected	491:498	arg2	matrix					437:442	acid-insoluble matrix	422:442	acid-insoluble matrix	422:442	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	3	65	located	detected	491:498	arg2	matrix					411:416	acid-soluble matrix	398:416	acid-soluble matrix	398:416	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	3	65	located	detected	491:498	arg1	study					346:350	this study	341:350	this study	341:350	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	5	66	theme	Willebrand	931:940	arg1	factor					942:947	von Willebrand factor	927:947	von Willebrand factor type A	927:954	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	2	67	from	protein	246:252	arg1	biomineralization					263:279	pearl biomineralization	257:279	pearl biomineralization	257:279	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	2	67	from	protein	246:252	arg1	applications					296:307	biomedical applications	285:307	biomedical applications	285:307	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	4	68	theme	pearl	714:718	arg1	proteomics					720:729	pearl proteomics	714:729	pearl proteomics	714:729	The results showed that a total of 190 proteins were identified in pearl proteomics, which was divided into eight categories by their potential biomineralization functions.					
35753678	5	69	theme	proteins-acidic	1054:1068	arg1	macromolecules					1070:1083	the "chitin-silk fibroin gel proteins-acidic macromolecules	1025:1083	the "chitin-silk fibroin gel proteins-acidic macromolecules" model	1025:1090	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	5	70	theme	pearl	839:843	arg1	proteins					852:859	pearl matrix proteins	839:859	pearl matrix proteins	839:859	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	7	71	theme	molecular	1632:1640	arg1	basis					1642:1646	the molecular basis	1628:1646	the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application	1628:1742	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	6	72	theme	anti-apoptotic	1424:1437	arg1	effects					1369:1375	neuro-regulation effects	1352:1375	neuro-regulation effects	1352:1375	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	72	theme	anti-apoptotic	1424:1437	arg1	effects					1439:1445	anti-inflammatory and anti-apoptotic effects	1402:1445	anti-inflammatory and anti-apoptotic effects	1402:1445	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	5	73	theme	biomineralization	1131:1147	arg1	process					1149:1155	the pearl biomineralization process	1121:1155	description the pearl biomineralization process	1109:1155	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	1	74	theme	valuable	142:149	arg1	jewelry					159:165	valuable organic jewelry	142:165	valuable organic jewelry	142:165	The freshwater pearl is one kind of valuable organic jewelry and traditional Chinese medicine (TCM).					
35753678	4	75	theme	proteins	686:693	arg1	total					673:677	a total	671:677	a total of 190 proteins	671:693	The results showed that a total of 190 proteins were identified in pearl proteomics, which was divided into eight categories by their potential biomineralization functions.					
35753678	6	76	theme	anti-inflammatory	1402:1418	arg1	effects					1369:1375	neuro-regulation effects	1352:1375	neuro-regulation effects	1352:1375	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	6	76	theme	anti-inflammatory	1402:1418	arg1	effects					1439:1445	anti-inflammatory and anti-apoptotic effects	1402:1445	anti-inflammatory and anti-apoptotic effects	1402:1445	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	2	77	from	applications	296:307	arg1	basis					230:234	the molecular basis	216:234	the molecular basis of matrix protein in pearl biomineralization and biomedical applications	216:307	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	2	77	from	applications	296:307	arg1	unknown					321:327	unknown	321:327	unknown	321:327	However, the molecular basis of matrix protein in pearl biomineralization and biomedical applications are largely unknown to date.					
35753678	3	78	theme	acid-insoluble	422:435	arg1	matrix					437:442	acid-insoluble matrix	422:442	acid-insoluble matrix	422:442	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	5	79	theme	type	949:952	arg1	domains					894:900	domains	894:900	domains like carbonic anhydrase	894:924	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	5	79	theme	type	949:952	arg1	A					954:954	von Willebrand factor type A	927:954	von Willebrand factor type A	927:954	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	3	80	theme	freshwater	453:462	arg1	powder					479:484	the freshwater seedless pearl powder	449:484	the freshwater seedless pearl powder	449:484	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	0	81	theme	pearl	33:37	arg1	powder					39:44	the freshwater pearl powder	18:44	the freshwater pearl powder	18:44	The proteomics of the freshwater pearl powder: Insights from biomineralization to biomedical application.					
35753678	7	82	theme	mollusc	1597:1603	arg1	biomineralization					1605:1621	mollusc biomineralization	1597:1621	mollusc biomineralization	1597:1621	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	7	83	theme	TCM	1728:1730	arg1	application					1732:1742	TCM application	1728:1742	TCM application	1728:1742	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	5	84	dep	description	1109:1119	arg1	process					1149:1155	the pearl biomineralization process	1121:1155	description the pearl biomineralization process	1109:1155	The composition of pearl matrix proteins and the high frequency conserved domains like carbonic anhydrase, von Willebrand factor type A, tyrosinase and chitin binding 2 in protein sequences, implying that the "chitin-silk fibroin gel proteins-acidic macromolecules" model was suitable for description the pearl biomineralization process.					
35753678	3	85	theme	sac	642:644	arg1	database					620:627	the transcriptomic database	601:627	the transcriptomic database of the pearl sac	601:644	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	6	86	theme	immunomodulatory	1457:1472	arg1	property					1474:1481	other immunomodulatory property	1451:1481	other immunomodulatory property	1451:1481	Meanwhile, ninety-one of pearl matrix proteins could be classified into seven categories by their potential medical functions including wound healing, osteogenic property, antioxidant activity, neuro-regulation effects, skin lightening effect, anti-inflammatory and anti-apoptotic effects and other immunomodulatory property.					
35753678	7	87	theme	protein	1585:1591	arg1	diversity					1559:1567	not only the diversity	1546:1567	not only the diversity of pearl matrix protein for mollusc biomineralization	1546:1621	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	3	88	theme	water-soluble	376:388	arg1	matrix					390:395	water-soluble matrix	376:395	water-soluble matrix	376:395	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	7	89	theme	pearl	1572:1576	arg1	protein					1585:1591	pearl matrix protein	1572:1591	pearl matrix protein for mollusc biomineralization	1572:1621	In general, these results provided valuable new insights into not only the diversity of pearl matrix protein for mollusc biomineralization, but the molecular basis of pearl matrix proteins responsible for their diverse biological properties in TCM application.					
35753678	3	90	theme	pearl	473:477	arg1	powder					479:484	the freshwater seedless pearl powder	449:484	the freshwater seedless pearl powder	449:484	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	0	91	dep	proteomics	4:13	arg1	Insights					47:54	Insights	47:54	The proteomics of the freshwater pearl powder: Insights from biomineralization to biomedical application.	0:104	The proteomics of the freshwater pearl powder: Insights from biomineralization to biomedical application.					
35753678	4	92	theme	biomineralization	791:807	arg1	functions					809:817	their potential biomineralization functions	775:817	their potential biomineralization functions	775:817	The results showed that a total of 190 proteins were identified in pearl proteomics, which was divided into eight categories by their potential biomineralization functions.					
35753678	3	93	theme	chromatography-tandem	513:533	arg1	LC-MS/MS					554:561	LC-MS/MS	554:561	LC-MS/MS	554:561	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35753678	3	93	theme	chromatography-tandem	513:533	arg1	spectrometry					540:551	liquid chromatography-tandem mass spectrometry	506:551	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	506:562	In this study, the matrix proteins of water-soluble matrix, acid-soluble matrix and acid-insoluble matrix from the freshwater seedless pearl powder were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) respectively, and identified against the transcriptomic database of the pearl sac.					
35461866	0	0	theme	effective	100:108	arg1	removal					110:116	effective removal	100:116	effective removal of uranyl ions from aqueous solution	100:153	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	2	1	theme	UO22+	421:425	arg1	concentration					431:443	the initial UO22+ ion concentration	409:443	the initial UO22+ ion concentration	409:443	During the study, we recorded and analyzed the initial UO22+ ion concentration, initial pH, contact time, temperature, and recovery.					
35461866	7	2	theme	UO22+	1126:1130	arg1	adsorption					1136:1145	the UO22+ ion adsorption	1122:1145	the UO22+ ion adsorption	1122:1145	The adsorption thermodynamic shown indicated that the UO22+ ion adsorption was both spontaneous and endothermic.					
35461866	7	2	theme	UO22+	1126:1130	arg1	spontaneous					1156:1166	spontaneous	1156:1166	spontaneous	1156:1166	The adsorption thermodynamic shown indicated that the UO22+ ion adsorption was both spontaneous and endothermic.					
35461866	9	3	theme	UO22+	1337:1341	arg1	ions					1343:1346	toxic UO22+ ions	1331:1346	toxic UO22+ ions	1331:1346	The study suggested a simple and cost-effective approach for the removal of toxic UO22+ ions from wastewater.					
35461866	4	4	theme	Ch-VL	646:650	arg1	composite					652:660	the Ch-VL composite	642:660	the Ch-VL composite	642:660	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	1	5	from	solutions	355:363	arg1	removal					318:324	the removal	314:324	the removal of the UO22+ ions in aqueous solutions	314:363	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	6	theme	Ch-VL	303:307	arg1	L					238:238	lignin (L)	230:239	lignin (L)	230:239	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	6	theme	Ch-VL	303:307	arg1	components					258:267	the components	254:267	the components of a natural composite adsorbent (Ch-VL)	254:308	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	6	theme	Ch-VL	303:307	arg1	vermiculite					210:220	vermiculite	210:220	vermiculite (V)	210:224	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	6	theme	Ch-VL	303:307	arg1	Chitosan					195:202	Chitosan	195:202	Chitosan (Ch)	195:207	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	7	7	theme	ion	1132:1134	arg1	adsorption					1136:1145	the UO22+ ion adsorption	1122:1145	the UO22+ ion adsorption	1122:1145	The adsorption thermodynamic shown indicated that the UO22+ ion adsorption was both spontaneous and endothermic.					
35461866	7	7	theme	ion	1132:1134	arg1	spontaneous					1156:1166	spontaneous	1156:1166	spontaneous	1156:1166	The adsorption thermodynamic shown indicated that the UO22+ ion adsorption was both spontaneous and endothermic.					
35461866	5	8	theme	maximum	863:869	arg1	capacity					882:889	Its maximum adsorption capacity	859:889	Its maximum adsorption capacity	859:889	Its maximum adsorption capacity was 0.322 mol kg-1, obtained from the Langmuir isotherm model.					
35461866	11	9	with	agreement	1469:1477	arg1	observations					1497:1508	experimental observations	1484:1508	experimental observations	1484:1508	Theoretical results are in good agreement with experimental observations.					
35461866	1	10	from	ions	339:342	arg1	solutions					355:363	aqueous solutions	347:363	aqueous solutions	347:363	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	9	11	theme	simple	1277:1282	arg1	approach					1303:1310	a simple and cost-effective approach	1275:1310	a simple and cost-effective approach for the removal of toxic UO22+ ions from wastewater	1275:1362	The study suggested a simple and cost-effective approach for the removal of toxic UO22+ ions from wastewater.					
35461866	5	12	theme	Langmuir	929:936	arg1	model					947:951	the Langmuir isotherm model	925:951	the Langmuir isotherm model	925:951	Its maximum adsorption capacity was 0.322 mol kg-1, obtained from the Langmuir isotherm model.					
35461866	6	13	theme	adsorption	958:967	arg1	kinetics					969:976	The adsorption kinetics	954:976	The adsorption kinetics	954:976	The adsorption kinetics indicated that it followed the pseudo-second-order and intraparticle diffusion rate kinetics.					
35461866	10	14	theme	adsorption	1382:1391	arg1	mechanism					1393:1401	the adsorption mechanism	1378:1401	the adsorption mechanism	1378:1401	To highlight the adsorption mechanism, DFT calculations were performed.					
35461866	4	15	from	temperature	706:716	arg1	600 mg L-1					681:690	600 mg L-1	681:690	600 mg L-1	681:690	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	5	16	theme	isotherm	938:945	arg1	model					947:951	the Langmuir isotherm model	925:951	the Langmuir isotherm model	925:951	Its maximum adsorption capacity was 0.322 mol kg-1, obtained from the Langmuir isotherm model.					
35461866	0	17	theme	ions	128:131	arg1	removal					110:116	effective removal	100:116	effective removal of uranyl ions from aqueous solution	100:153	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	5	18	theme	adsorption	871:880	arg1	capacity					882:889	Its maximum adsorption capacity	859:889	Its maximum adsorption capacity	859:889	Its maximum adsorption capacity was 0.322 mol kg-1, obtained from the Langmuir isotherm model.					
35461866	9	19	from	wastewater	1353:1362	arg1	removal					1320:1326	the removal	1316:1326	the removal of toxic UO22+ ions from wastewater	1316:1362	The study suggested a simple and cost-effective approach for the removal of toxic UO22+ ions from wastewater.					
35461866	4	20	theme	25 °C.	721:726	arg1	findings					742:749	25 °C. Thermodynamic findings	721:749	25 °C. Thermodynamic findings	721:749	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	9	21	theme	toxic	1331:1335	arg1	ions					1343:1346	toxic UO22+ ions	1331:1346	toxic UO22+ ions	1331:1346	The study suggested a simple and cost-effective approach for the removal of toxic UO22+ ions from wastewater.					
35461866	0	22	theme	uranyl	121:126	arg1	ions					128:131	uranyl ions	121:131	uranyl ions	121:131	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	2	23	theme	initial	413:419	arg1	concentration					431:443	the initial UO22+ ion concentration	409:443	the initial UO22+ ion concentration	409:443	During the study, we recorded and analyzed the initial UO22+ ion concentration, initial pH, contact time, temperature, and recovery.					
35461866	4	24	from	pH 4.5	695:700	arg1	600 mg L-1					681:690	600 mg L-1	681:690	600 mg L-1	681:690	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	2	25	theme	initial	446:452	arg1	pH					454:455	initial pH	446:455	initial pH	446:455	During the study, we recorded and analyzed the initial UO22+ ion concentration, initial pH, contact time, temperature, and recovery.					
35461866	1	26	from	removal	318:324	arg1	solutions					355:363	aqueous solutions	347:363	aqueous solutions	347:363	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	27	used	used	246:249	arg2	L					238:238	lignin (L)	230:239	lignin (L)	230:239	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	27	used	used	246:249	arg2	vermiculite					210:220	vermiculite	210:220	vermiculite (V)	210:224	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	27	used	used	246:249	arg2	components					258:267	the components	254:267	the components of a natural composite adsorbent (Ch-VL)	254:308	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	27	used	used	246:249	arg2	Chitosan					195:202	Chitosan	195:202	Chitosan (Ch)	195:207	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	27	used	used	246:249	arg2	Ch					205:206	Ch	205:206	Ch	205:206	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	27	used	used	246:249	arg2	V					223:223	V	223:223	V	223:223	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	4	28	theme	adsorption	806:815	arg1	behavior					817:824	adsorption behavior	806:824	adsorption behavior	806:824	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	4	29	theme	Adsorption	616:625	arg1	performance					627:637	Adsorption performance	616:637	Adsorption performance of the Ch-VL composite for UO22+ ions	616:675	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	8	30	theme	adsorption	1189:1198	arg1	process					1200:1206	The adsorption process	1185:1206	The adsorption process	1185:1206	The adsorption process was enlightened by FT-IR and SEM-EDX analyses.					
35461866	9	31	theme	ions	1343:1346	arg1	removal					1320:1326	the removal	1316:1326	the removal of toxic UO22+ ions from wastewater	1316:1362	The study suggested a simple and cost-effective approach for the removal of toxic UO22+ ions from wastewater.					
35461866	9	32	theme	cost-effective	1288:1301	arg1	approach					1303:1310	a simple and cost-effective approach	1275:1310	a simple and cost-effective approach for the removal of toxic UO22+ ions from wastewater	1275:1362	The study suggested a simple and cost-effective approach for the removal of toxic UO22+ ions from wastewater.					
35461866	11	33	theme	experimental	1484:1495	arg1	observations					1497:1508	experimental observations	1484:1508	experimental observations	1484:1508	Theoretical results are in good agreement with experimental observations.					
35461866	3	34	theme	sequential	570:579	arg1	experiments					603:613	three sequential adsorption/desorption experiments	564:613	three sequential adsorption/desorption experiments	564:613	The recycling performance of the Ch-VL composite was assessed by three sequential adsorption/desorption experiments.					
35461866	0	35	theme	aqueous	138:144	arg1	solution					146:153	aqueous solution	138:153	aqueous solution	138:153	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	6	36	theme	diffusion	1047:1055	arg1	kinetics					1062:1069	the pseudo-second-order and intraparticle diffusion rate kinetics	1005:1069	the pseudo-second-order and intraparticle diffusion rate kinetics	1005:1069	The adsorption kinetics indicated that it followed the pseudo-second-order and intraparticle diffusion rate kinetics.					
35461866	11	37	theme	Theoretical	1437:1447	arg1	results					1449:1455	Theoretical results	1437:1455	Theoretical results	1437:1455	Theoretical results are in good agreement with experimental observations.					
35461866	6	38	theme	intraparticle	1033:1045	arg1	kinetics					1062:1069	the pseudo-second-order and intraparticle diffusion rate kinetics	1005:1069	the pseudo-second-order and intraparticle diffusion rate kinetics	1005:1069	The adsorption kinetics indicated that it followed the pseudo-second-order and intraparticle diffusion rate kinetics.					
35461866	0	39	theme	ternary	62:68	arg1	composite					70:78	chitosan-vermiculite-lignin ternary composite	34:78	chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution	34:153	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	11	40	theme	good	1464:1467	arg1	agreement					1469:1477	good agreement	1464:1477	good agreement with experimental observations	1464:1508	Theoretical results are in good agreement with experimental observations.					
35461866	8	41	dep	FT-IR	1227:1231	arg1	analyses					1245:1252	analyses	1245:1252	analyses	1245:1252	The adsorption process was enlightened by FT-IR and SEM-EDX analyses.					
35461866	1	42	theme	UO22+	333:337	arg1	ions					339:342	the UO22+ ions	329:342	the UO22+ ions in aqueous solutions	329:363	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	6	43	theme	rate	1057:1060	arg1	kinetics					1062:1069	the pseudo-second-order and intraparticle diffusion rate kinetics	1005:1069	the pseudo-second-order and intraparticle diffusion rate kinetics	1005:1069	The adsorption kinetics indicated that it followed the pseudo-second-order and intraparticle diffusion rate kinetics.					
35461866	0	44	theme	chitosan-vermiculite-lignin	34:60	arg1	composite					70:78	chitosan-vermiculite-lignin ternary composite	34:78	chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution	34:153	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	2	45	theme	contact	458:464	arg1	time					466:469	contact time	458:469	contact time	458:469	During the study, we recorded and analyzed the initial UO22+ ion concentration, initial pH, contact time, temperature, and recovery.					
35461866	1	46	theme	ions	339:342	arg1	removal					318:324	the removal	314:324	the removal of the UO22+ ions in aqueous solutions	314:363	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	6	47	theme	pseudo-second-order	1009:1027	arg1	kinetics					1062:1069	the pseudo-second-order and intraparticle diffusion rate kinetics	1005:1069	the pseudo-second-order and intraparticle diffusion rate kinetics	1005:1069	The adsorption kinetics indicated that it followed the pseudo-second-order and intraparticle diffusion rate kinetics.					
35461866	7	48	theme	adsorption	1076:1085	arg1	thermodynamic					1087:1099	The adsorption thermodynamic	1072:1099	The adsorption thermodynamic shown	1072:1105	The adsorption thermodynamic shown indicated that the UO22+ ion adsorption was both spontaneous and endothermic.					
35461866	4	49	from	ΔG0	775:777	arg1	600 mg L-1					681:690	600 mg L-1	681:690	600 mg L-1	681:690	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	0	50	dep	analyses	185:192	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	0	50	dep	analyses	185:192	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	0	50	dep	analyses	185:192	arg1	analyses					185:192	Experimental and theoretical analyses	156:192	Experimental and theoretical analyses	156:192	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	0	51	theme	composite	70:78	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	0	51	theme	composite	70:78	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	0	51	theme	composite	70:78	arg1	analyses					185:192	Experimental and theoretical analyses	156:192	Experimental and theoretical analyses	156:192	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	1	52	theme	natural	274:280	arg1	Ch-VL					303:307	a natural composite adsorbent (Ch-VL)	272:308	a natural composite adsorbent (Ch-VL)	272:308	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	0	53	theme	Experimental	156:167	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	0	53	theme	Experimental	156:167	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	0	53	theme	Experimental	156:167	arg1	analyses					185:192	Experimental and theoretical analyses	156:192	Experimental and theoretical analyses	156:192	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	1	54	theme	aqueous	347:353	arg1	solutions					355:363	aqueous solutions	347:363	aqueous solutions	347:363	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	3	55	theme	recycling	503:511	arg1	performance					513:523	The recycling performance	499:523	The recycling performance of the Ch-VL composite	499:546	The recycling performance of the Ch-VL composite was assessed by three sequential adsorption/desorption experiments.					
35461866	4	56	theme	composite	652:660	arg1	performance					627:637	Adsorption performance	616:637	Adsorption performance of the Ch-VL composite for UO22+ ions	616:675	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	1	57	theme	composite	282:290	arg1	Ch-VL					303:307	a natural composite adsorbent (Ch-VL)	272:308	a natural composite adsorbent (Ch-VL)	272:308	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	4	58	theme	findings	742:749	arg1	temperature					706:716	temperature	706:716	temperature	706:716	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	4	58	theme	findings	742:749	arg1	pH 4.5					695:700	pH 4.5	695:700	pH 4.5	695:700	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	4	58	theme	findings	742:749	arg1	ΔG0					775:777	ΔG0	775:777	ΔG0	775:777	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	4	58	theme	findings	742:749	arg1	ΔH0:28.1 kJ mol-1					752:768	ΔH0:28.1 kJ mol-1	752:768	ΔH0:28.1 kJ mol-1	752:768	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	3	59	theme	composite	538:546	arg1	performance					513:523	The recycling performance	499:523	The recycling performance of the Ch-VL composite	499:546	The recycling performance of the Ch-VL composite was assessed by three sequential adsorption/desorption experiments.					
35461866	10	60	theme	DFT	1404:1406	arg1	calculations					1408:1419	DFT calculations	1404:1419	DFT calculations	1404:1419	To highlight the adsorption mechanism, DFT calculations were performed.					
35461866	0	61	theme	theoretical	173:183	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	0	61	theme	theoretical	173:183	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	0	61	theme	theoretical	173:183	arg1	analyses					185:192	Experimental and theoretical analyses	156:192	Experimental and theoretical analyses	156:192	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	3	62	theme	adsorption/desorption	581:601	arg1	experiments					603:613	three sequential adsorption/desorption experiments	564:613	three sequential adsorption/desorption experiments	564:613	The recycling performance of the Ch-VL composite was assessed by three sequential adsorption/desorption experiments.					
35461866	4	63	theme	Thermodynamic	728:740	arg1	findings					742:749	25 °C. Thermodynamic findings	721:749	25 °C. Thermodynamic findings	721:749	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	4	64	from	ΔH0:28.1 kJ mol-1	752:768	arg1	600 mg L-1					681:690	600 mg L-1	681:690	600 mg L-1	681:690	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	4	65	theme	UO22+	666:670	arg1	ions					672:675	UO22+ ions	666:675	UO22+ ions	666:675	Adsorption performance of the Ch-VL composite for UO22+ ions was 600 mg L-1 at pH 4.5 and temperature of 25 °C. Thermodynamic findings, ΔH0:28.1 kJ mol-1, and ΔG0:-14.1 kJ mol-1 showed that adsorption behavior was endothermic and spontaneous.					
35461866	0	66	from	solution	146:153	arg1	removal					110:116	effective removal	100:116	effective removal of uranyl ions from aqueous solution	100:153	Synthesis and characterization of chitosan-vermiculite-lignin ternary composite as an adsorbent for effective removal of uranyl ions from aqueous solution: Experimental and theoretical analyses.					
35461866	2	67	theme	ion	427:429	arg1	concentration					431:443	the initial UO22+ ion concentration	409:443	the initial UO22+ ion concentration	409:443	During the study, we recorded and analyzed the initial UO22+ ion concentration, initial pH, contact time, temperature, and recovery.					
35461866	1	68	theme	adsorbent	292:300	arg1	Ch-VL					303:307	a natural composite adsorbent (Ch-VL)	272:308	a natural composite adsorbent (Ch-VL)	272:308	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	3	69	theme	Ch-VL	532:536	arg1	composite					538:546	the Ch-VL composite	528:546	the Ch-VL composite	528:546	The recycling performance of the Ch-VL composite was assessed by three sequential adsorption/desorption experiments.					
35461866	1	70	theme	lignin	230:235	arg1	L					238:238	lignin (L)	230:239	lignin (L)	230:239	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	70	theme	lignin	230:235	arg1	components					258:267	the components	254:267	the components of a natural composite adsorbent (Ch-VL)	254:308	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	70	theme	lignin	230:235	arg1	vermiculite					210:220	vermiculite	210:220	vermiculite (V)	210:224	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
35461866	1	70	theme	lignin	230:235	arg1	Chitosan					195:202	Chitosan	195:202	Chitosan (Ch)	195:207	Chitosan (Ch), vermiculite (V) and lignin (L) were used as the components of a natural composite adsorbent (Ch-VL) for the removal of the UO22+ ions in aqueous solutions.					
36536283	9	0	from	composition	1372:1382	arg1	soil					1387:1390	soil	1387:1390	soil	1387:1390	The bacterial composition in soil fluctuated over time with or without the introduction of either type of plastic.					
36536283	7	1	theme	region	1187:1192	arg1	sequencing					1163:1172	Illumina high-throughput sequencing	1138:1172	Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene	1138:1208	Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.					
36536283	4	2	from	dynamics	645:652	arg1	soil					680:683	soil	680:683	soil	680:683	In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE).					
36536283	10	3	from	treatment	1526:1534	arg1	study					1544:1548	this study	1539:1548	this study	1539:1548	While major bacterial phyla remained similar for all treatment in this study, different types of plastics led to different soil bacterial community structure.					
36536283	14	4	theme	contrasting	2131:2141	arg1	shift					2143:2147	contrasting shift	2131:2147	contrasting shift in soil bacterial population overtime, which may determine their fate in the environment	2131:2236	CONCLUSIONS The introduction of starch-based bioplastic and oxo-LDPE led to contrasting shift in soil bacterial population overtime, which may determine their fate in the environment.					
36536283	12	5	theme	those	1923:1927	arg1	population					1794:1803	the population	1790:1803	the population of bacteria known for their ability to directly utilize plastic component for their growth	1790:1894	The introduction of plastics into soil increased not only the population of bacteria known for their ability to directly utilize plastic component for their growth, but also the abundance of those that may interact with direct degraders.					
36536283	12	5	theme	those	1923:1927	arg1	abundance					1910:1918	the abundance	1906:1918	the abundance of those that may interact with direct degraders	1906:1967	The introduction of plastics into soil increased not only the population of bacteria known for their ability to directly utilize plastic component for their growth, but also the abundance of those that may interact with direct degraders.					
36536283	1	6	theme	waste	133:137	arg1	accumulation					139:150	BACKGROUND Plastic waste accumulation	114:150	BACKGROUND Plastic waste accumulation	114:150	BACKGROUND Plastic waste accumulation is one of the main ecological concerns in the past decades.					
36536283	10	7	theme	bacterial	1485:1493	arg1	phyla					1495:1499	major bacterial phyla	1479:1499	major bacterial phyla	1479:1499	While major bacterial phyla remained similar for all treatment in this study, different types of plastics led to different soil bacterial community structure.					
36536283	1	8	theme	past	198:201	arg1	decades					203:209	the past decades	194:209	the past decades	194:209	BACKGROUND Plastic waste accumulation is one of the main ecological concerns in the past decades.					
36536283	14	9	theme	starch-based	2087:2098	arg1	bioplastic					2100:2109	starch-based bioplastic	2087:2109	starch-based bioplastic	2087:2109	CONCLUSIONS The introduction of starch-based bioplastic and oxo-LDPE led to contrasting shift in soil bacterial population overtime, which may determine their fate in the environment.					
36536283	11	10	theme	specific	1710:1717	arg1	points					1724:1729	specific time points	1710:1729	specific time points	1710:1729	None of these bacteria were abundant continuously, but rather they emerged at specific time points.					
36536283	2	11	theme	plastics	232:239	arg1	generation					218:227	A new generation	212:227	A new generation of plastics that are easier to degrade in the environment compared to conventional plastics, such as starch-based bioplastics and oxo-biodegradable plastics,	212:385	A new generation of plastics that are easier to degrade in the environment compared to conventional plastics, such as starch-based bioplastics and oxo-biodegradable plastics, is perceived as a solution to this issue.					
36536283	2	11	theme	plastics	232:239	arg1	solution					405:412	a solution	403:412	a solution to this issue	403:426	A new generation of plastics that are easier to degrade in the environment compared to conventional plastics, such as starch-based bioplastics and oxo-biodegradable plastics, is perceived as a solution to this issue.					
36536283	1	12	from	decades	203:209	arg1	ecological					171:180	ecological	171:180	ecological	171:180	BACKGROUND Plastic waste accumulation is one of the main ecological concerns in the past decades.					
36536283	8	13	theme	bioplastic	1252:1261	arg1	weight					1263:1268	the starch-based bioplastic weight	1235:1268	the starch-based bioplastic weight has	1235:1272	RESULTS After 120 days, the starch-based bioplastic weight has decreased by 74%, while the oxo-LDPE remained intact with only 3% weight reduction.					
36536283	7	14	theme	V3-V4	1181:1185	arg1	region					1187:1192	the V3-V4 region	1177:1192	the V3-V4 region of 16SrRNA gene	1177:1208	Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.					
36536283	4	15	theme	bacterial	657:665	arg1	community					667:675	bacterial community	657:675	bacterial community	657:675	In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE).					
36536283	8	16	with	intact	1320:1325	arg1	reduction					1347:1355	only 3% weight reduction	1332:1355	only 3% weight reduction	1332:1355	RESULTS After 120 days, the starch-based bioplastic weight has decreased by 74%, while the oxo-LDPE remained intact with only 3% weight reduction.					
36536283	1	17	theme	ecological	171:180	arg1	one					155:157	one	155:157	one	155:157	BACKGROUND Plastic waste accumulation is one of the main ecological concerns in the past decades.					
36536283	1	17	theme	ecological	171:180	arg1	ecological					171:180	ecological	171:180	ecological	171:180	BACKGROUND Plastic waste accumulation is one of the main ecological concerns in the past decades.					
36536283	7	18	theme	high-throughput	1147:1161	arg1	sequencing					1163:1172	Illumina high-throughput sequencing	1138:1172	Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene	1138:1208	Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.					
36536283	7	19	attach	attached	1073:1080	arg2	soil					1068:1071	soil	1068:1071	soil attached to the surface of remaining plastics	1068:1117	Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.					
36536283	7	19	attach	attached	1073:1080	arg1	surface					1089:1095	the surface	1085:1095	the surface of remaining plastics	1085:1117	Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.					
36536283	4	20	theme	biodegradable	741:753	arg1	bioplastic					777:786	biodegradable: cassava starch-based bioplastic	741:786	biodegradable: cassava starch-based bioplastic	741:786	In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE).					
36536283	12	21	theme	bacteria	1808:1815	arg1	population					1794:1803	the population	1790:1803	the population of bacteria known for their ability to directly utilize plastic component for their growth	1790:1894	The introduction of plastics into soil increased not only the population of bacteria known for their ability to directly utilize plastic component for their growth, but also the abundance of those that may interact with direct degraders.					
36536283	12	21	theme	bacteria	1808:1815	arg1	abundance					1910:1918	the abundance	1906:1918	the abundance of those that may interact with direct degraders	1906:1967	The introduction of plastics into soil increased not only the population of bacteria known for their ability to directly utilize plastic component for their growth, but also the abundance of those that may interact with direct degraders.					
36536283	0	22	from	burial	30:35	arg1	soil					108:111	compost soil	100:111	compost soil	100:111	Bacterial dynamics during the burial of starch-based bioplastic and oxo-low-density-polyethylene in compost soil.					
36536283	0	23	theme	Bacterial	0:8	arg1	dynamics					10:17	Bacterial dynamics	0:17	Bacterial dynamics during the burial of starch-based bioplastic and oxo-low-density-polyethylene in compost soil.	0:112	Bacterial dynamics during the burial of starch-based bioplastic and oxo-low-density-polyethylene in compost soil.					
36536283	4	24	theme	carrier	717:723	arg1	bags					725:728	commercial carrier bags	706:728	commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE)	706:830	In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE).					
36536283	10	25	theme	bacterial	1601:1609	arg1	structure					1621:1629	different soil bacterial community structure	1586:1629	different soil bacterial community structure	1586:1629	While major bacterial phyla remained similar for all treatment in this study, different types of plastics led to different soil bacterial community structure.					
36536283	7	26	theme	remaining	1100:1108	arg1	plastics					1110:1117	remaining plastics	1100:1117	remaining plastics	1100:1117	Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.					
36536283	1	27	theme	Plastic	125:131	arg1	accumulation					139:150	BACKGROUND Plastic waste accumulation	114:150	BACKGROUND Plastic waste accumulation	114:150	BACKGROUND Plastic waste accumulation is one of the main ecological concerns in the past decades.					
36536283	4	28	theme	oxo-low-density	792:806	arg1	oxo-LDPE					822:829	oxo-LDPE	822:829	oxo-LDPE	822:829	In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE).					
36536283	4	28	theme	oxo-low-density	792:806	arg1	polyethylene					808:819	oxo-low-density polyethylene	792:819	oxo-low-density polyethylene (oxo-LDPE)	792:830	In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE).					
36536283	10	29	theme	different	1586:1594	arg1	structure					1621:1629	different soil bacterial community structure	1586:1629	different soil bacterial community structure	1586:1629	While major bacterial phyla remained similar for all treatment in this study, different types of plastics led to different soil bacterial community structure.					
36536283	7	30	from	diversity	1055:1063	arg1	soil					1068:1071	soil	1068:1071	soil attached to the surface of remaining plastics	1068:1117	Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.					
36536283	0	31	theme	starch-based	40:51	arg1	bioplastic					53:62	starch-based bioplastic	40:62	starch-based bioplastic	40:62	Bacterial dynamics during the burial of starch-based bioplastic and oxo-low-density-polyethylene in compost soil.					
36536283	8	32	dep	weight	1263:1268	arg1	has					1270:1272	has	1270:1272	has	1270:1272	RESULTS After 120 days, the starch-based bioplastic weight has decreased by 74%, while the oxo-LDPE remained intact with only 3% weight reduction.					
36536283	12	33	theme	plastics	1752:1759	arg1	introduction					1736:1747	The introduction	1732:1747	The introduction of plastics into soil	1732:1769	The introduction of plastics into soil increased not only the population of bacteria known for their ability to directly utilize plastic component for their growth, but also the abundance of those that may interact with direct degraders.					
36536283	4	34	theme	cassava	756:762	arg1	bioplastic					777:786	biodegradable: cassava starch-based bioplastic	741:786	biodegradable: cassava starch-based bioplastic	741:786	In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE).					
36536283	10	35	theme	major	1479:1483	arg1	phyla					1495:1499	major bacterial phyla	1479:1499	major bacterial phyla	1479:1499	While major bacterial phyla remained similar for all treatment in this study, different types of plastics led to different soil bacterial community structure.					
36536283	7	36	theme	gene	1205:1208	arg1	region					1187:1192	the V3-V4 region	1177:1192	the V3-V4 region of 16SrRNA gene	1177:1208	Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.					
36536283	14	37	theme	soil	2152:2155	arg1	overtime					2178:2185	soil bacterial population overtime	2152:2185	soil bacterial population overtime	2152:2185	CONCLUSIONS The introduction of starch-based bioplastic and oxo-LDPE led to contrasting shift in soil bacterial population overtime, which may determine their fate in the environment.					
36536283	2	38	theme	oxo-biodegradable	359:375	arg1	plastics					377:384	oxo-biodegradable plastics	359:384	oxo-biodegradable plastics	359:384	A new generation of plastics that are easier to degrade in the environment compared to conventional plastics, such as starch-based bioplastics and oxo-biodegradable plastics, is perceived as a solution to this issue.					
36536283	6	39	theme	plastics	1021:1028	arg1	weight					1001:1006	the weight	997:1006	soil pH and temperature as well as the weight of remaining plastics	962:1028	Following incubation, soil pH and temperature as well as the weight of remaining plastics were measured.					
36536283	6	39	theme	plastics	1021:1028	arg1	temperature					974:984	temperature	974:984	temperature	974:984	Following incubation, soil pH and temperature as well as the weight of remaining plastics were measured.					
36536283	6	39	theme	plastics	1021:1028	arg1	pH					967:968	soil pH	962:968	soil pH	962:968	Following incubation, soil pH and temperature as well as the weight of remaining plastics were measured.					
36536283	7	40	theme	Bacterial	1045:1053	arg1	diversity					1055:1063	Bacterial diversity	1045:1063	Bacterial diversity in soil attached to the surface of remaining plastics	1045:1117	Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.					
36536283	9	41	theme	bacterial	1362:1370	arg1	composition					1372:1382	The bacterial composition	1358:1382	The bacterial composition in soil	1358:1390	The bacterial composition in soil fluctuated over time with or without the introduction of either type of plastic.					
36536283	14	42	theme	oxo-LDPE	2115:2122	arg1	introduction					2071:2082	The introduction	2067:2082	The introduction of starch-based bioplastic and oxo-LDPE	2067:2122	CONCLUSIONS The introduction of starch-based bioplastic and oxo-LDPE led to contrasting shift in soil bacterial population overtime, which may determine their fate in the environment.					
36536283	5	43	theme	plastic	846:852	arg1	bag					854:856	plastic bag	846:856	plastic bag	846:856	Each type of plastic bag was buried separately in compost soil and incubated for 30, 60, 90, and 120 days.					
36536283	2	44	theme	new	214:216	arg1	generation					218:227	A new generation	212:227	A new generation of plastics that are easier to degrade in the environment compared to conventional plastics, such as starch-based bioplastics and oxo-biodegradable plastics,	212:385	A new generation of plastics that are easier to degrade in the environment compared to conventional plastics, such as starch-based bioplastics and oxo-biodegradable plastics, is perceived as a solution to this issue.					
36536283	2	44	theme	new	214:216	arg1	solution					405:412	a solution	403:412	a solution to this issue	403:426	A new generation of plastics that are easier to degrade in the environment compared to conventional plastics, such as starch-based bioplastics and oxo-biodegradable plastics, is perceived as a solution to this issue.					
36536283	14	45	theme	bioplastic	2100:2109	arg1	introduction					2071:2082	The introduction	2067:2082	The introduction of starch-based bioplastic and oxo-LDPE	2067:2122	CONCLUSIONS The introduction of starch-based bioplastic and oxo-LDPE led to contrasting shift in soil bacterial population overtime, which may determine their fate in the environment.					
36536283	2	46	theme	conventional	299:310	arg1	plastics					312:319	conventional plastics	299:319	conventional plastics	299:319	A new generation of plastics that are easier to degrade in the environment compared to conventional plastics, such as starch-based bioplastics and oxo-biodegradable plastics, is perceived as a solution to this issue.					
36536283	10	47	theme	different	1551:1559	arg1	types					1561:1565	different types	1551:1565	different types of plastics	1551:1577	While major bacterial phyla remained similar for all treatment in this study, different types of plastics led to different soil bacterial community structure.					
36536283	11	48	theme	time	1719:1722	arg1	points					1724:1729	specific time points	1710:1729	specific time points	1710:1729	None of these bacteria were abundant continuously, but rather they emerged at specific time points.					
36536283	5	49	theme	bag	854:856	arg1	type					838:841	Each type	833:841	Each type of plastic bag	833:856	Each type of plastic bag was buried separately in compost soil and incubated for 30, 60, 90, and 120 days.					
36536283	12	50	theme	plastic	1861:1867	arg1	component					1869:1877	plastic component	1861:1877	plastic component for their growth	1861:1894	The introduction of plastics into soil increased not only the population of bacteria known for their ability to directly utilize plastic component for their growth, but also the abundance of those that may interact with direct degraders.					
36536283	5	51	theme	compost	883:889	arg1	soil					891:894	compost soil	883:894	compost soil	883:894	Each type of plastic bag was buried separately in compost soil and incubated for 30, 60, 90, and 120 days.					
36536283	4	52	theme	community	667:675	arg1	dynamics					645:652	the dynamics	641:652	the dynamics of bacterial community in soil	641:683	In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE).					
36536283	13	53	theme	nitrogen	2008:2015	arg1	cycling					2017:2023	nitrogen cycling	2008:2023	nitrogen cycling	2008:2023	Bacterial groups that are involved in nitrogen cycling also arose throughout burial.					
36536283	8	54	dep	reduction	1347:1355	arg1	%					1338:1338	%	1338:1338	%	1338:1338	RESULTS After 120 days, the starch-based bioplastic weight has decreased by 74%, while the oxo-LDPE remained intact with only 3% weight reduction.					
36536283	8	55	theme	starch-based	1239:1250	arg1	weight					1263:1268	the starch-based bioplastic weight	1235:1268	the starch-based bioplastic weight has	1235:1272	RESULTS After 120 days, the starch-based bioplastic weight has decreased by 74%, while the oxo-LDPE remained intact with only 3% weight reduction.					
36536283	0	56	theme	oxo-low-density-polyethylene	68:95	arg1	burial					30:35	the burial	26:35	the burial of starch-based bioplastic and oxo-low-density-polyethylene in compost soil	26:111	Bacterial dynamics during the burial of starch-based bioplastic and oxo-low-density-polyethylene in compost soil.					
36536283	8	57	dep	%	1338:1338	arg1	3					1337:1337	3	1337:1337	3	1337:1337	RESULTS After 120 days, the starch-based bioplastic weight has decreased by 74%, while the oxo-LDPE remained intact with only 3% weight reduction.					
36536283	6	58	theme	soil	962:965	arg1	pH					967:968	soil pH	962:968	soil pH	962:968	Following incubation, soil pH and temperature as well as the weight of remaining plastics were measured.					
36536283	12	59	theme	direct	1952:1957	arg1	degraders					1959:1967	direct degraders	1952:1967	direct degraders	1952:1967	The introduction of plastics into soil increased not only the population of bacteria known for their ability to directly utilize plastic component for their growth, but also the abundance of those that may interact with direct degraders.					
36536283	0	60	theme	compost	100:106	arg1	soil					108:111	compost soil	100:111	compost soil	100:111	Bacterial dynamics during the burial of starch-based bioplastic and oxo-low-density-polyethylene in compost soil.					
36536283	9	61	theme	type	1456:1459	arg1	introduction					1433:1444	the introduction	1429:1444	the introduction of either type of plastic	1429:1470	The bacterial composition in soil fluctuated over time with or without the introduction of either type of plastic.					
36536283	7	62	theme	Illumina	1138:1145	arg1	sequencing					1163:1172	Illumina high-throughput sequencing	1138:1172	Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene	1138:1208	Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.					
36536283	1	63	from	ecological	171:180	arg1	decades					203:209	the past decades	194:209	the past decades	194:209	BACKGROUND Plastic waste accumulation is one of the main ecological concerns in the past decades.					
36536283	9	64	theme	plastic	1464:1470	arg1	type					1456:1459	either type	1449:1459	either type of plastic	1449:1470	The bacterial composition in soil fluctuated over time with or without the introduction of either type of plastic.					
36536283	14	65	from	shift	2143:2147	arg1	overtime					2178:2185	soil bacterial population overtime	2152:2185	soil bacterial population overtime	2152:2185	CONCLUSIONS The introduction of starch-based bioplastic and oxo-LDPE led to contrasting shift in soil bacterial population overtime, which may determine their fate in the environment.					
36536283	4	66	theme	bags	725:728	arg1	introduction					690:701	introduction	690:701	introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE)	690:830	In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE).					
36536283	8	67	dep	RESULTS	1211:1217	arg1	decreased					1274:1282	decreased	1274:1282	decreased	1274:1282	RESULTS After 120 days, the starch-based bioplastic weight has decreased by 74%, while the oxo-LDPE remained intact with only 3% weight reduction.					
36536283	6	68	theme	remaining	1011:1019	arg1	plastics					1021:1028	remaining plastics	1011:1028	remaining plastics	1011:1028	Following incubation, soil pH and temperature as well as the weight of remaining plastics were measured.					
36536283	10	69	theme	plastics	1570:1577	arg1	types					1561:1565	different types	1551:1565	different types of plastics	1551:1577	While major bacterial phyla remained similar for all treatment in this study, different types of plastics led to different soil bacterial community structure.					
36536283	7	70	theme	plastics	1110:1117	arg1	surface					1089:1095	the surface	1085:1095	the surface of remaining plastics	1085:1117	Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.					
36536283	13	71	theme	Bacterial	1970:1978	arg1	groups					1980:1985	Bacterial groups	1970:1985	Bacterial groups that are involved in nitrogen cycling	1970:2023	Bacterial groups that are involved in nitrogen cycling also arose throughout burial.					
36536283	14	72	theme	population	2167:2176	arg1	overtime					2178:2185	soil bacterial population overtime	2152:2185	soil bacterial population overtime	2152:2185	CONCLUSIONS The introduction of starch-based bioplastic and oxo-LDPE led to contrasting shift in soil bacterial population overtime, which may determine their fate in the environment.					
36536283	14	73	dep	CONCLUSIONS	2055:2065	arg1	led					2124:2126	led	2124:2126	led to contrasting shift in soil bacterial population overtime, which may determine their fate in the environment	2124:2236	CONCLUSIONS The introduction of starch-based bioplastic and oxo-LDPE led to contrasting shift in soil bacterial population overtime, which may determine their fate in the environment.					
36536283	1	74	dep	ecological	171:180	arg1	concerns					182:189	concerns	182:189	concerns	182:189	BACKGROUND Plastic waste accumulation is one of the main ecological concerns in the past decades.					
36536283	1	75	theme	BACKGROUND	114:123	arg1	accumulation					139:150	BACKGROUND Plastic waste accumulation	114:150	BACKGROUND Plastic waste accumulation	114:150	BACKGROUND Plastic waste accumulation is one of the main ecological concerns in the past decades.					
36536283	4	76	theme	commercial	706:715	arg1	bags					725:728	commercial carrier bags	706:728	commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE)	706:830	In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE).					
36536283	10	77	theme	soil	1596:1599	arg1	structure					1621:1629	different soil bacterial community structure	1586:1629	different soil bacterial community structure	1586:1629	While major bacterial phyla remained similar for all treatment in this study, different types of plastics led to different soil bacterial community structure.					
36536283	3	78	from	fate	442:445	arg1	environment					473:483	the environment	469:483	the environment	469:483	However, the fate of these materials in the environment are unclear, and less is known about how their presence affect the microorganisms that may play a role in their biodegradation.					
36536283	0	79	theme	bioplastic	53:62	arg1	burial					30:35	the burial	26:35	the burial of starch-based bioplastic and oxo-low-density-polyethylene in compost soil	26:111	Bacterial dynamics during the burial of starch-based bioplastic and oxo-low-density-polyethylene in compost soil.					
36536283	3	80	theme	materials	456:464	arg1	unclear					489:495	unclear	489:495	unclear	489:495	However, the fate of these materials in the environment are unclear, and less is known about how their presence affect the microorganisms that may play a role in their biodegradation.					
36536283	3	80	theme	materials	456:464	arg1	fate					442:445	the fate	438:445	the fate of these materials in the environment	438:483	However, the fate of these materials in the environment are unclear, and less is known about how their presence affect the microorganisms that may play a role in their biodegradation.					
36536283	11	81	theme	bacteria	1646:1653	arg1	None					1632:1635	None	1632:1635	None of these bacteria	1632:1653	None of these bacteria were abundant continuously, but rather they emerged at specific time points.					
36536283	2	82	theme	starch-based	330:341	arg1	bioplastics					343:353	starch-based bioplastics	330:353	starch-based bioplastics	330:353	A new generation of plastics that are easier to degrade in the environment compared to conventional plastics, such as starch-based bioplastics and oxo-biodegradable plastics, is perceived as a solution to this issue.					
36536283	8	83	theme	weight	1340:1345	arg1	reduction					1347:1355	only 3% weight reduction	1332:1355	only 3% weight reduction	1332:1355	RESULTS After 120 days, the starch-based bioplastic weight has decreased by 74%, while the oxo-LDPE remained intact with only 3% weight reduction.					
36536283	10	84	theme	community	1611:1619	arg1	structure					1621:1629	different soil bacterial community structure	1586:1629	different soil bacterial community structure	1586:1629	While major bacterial phyla remained similar for all treatment in this study, different types of plastics led to different soil bacterial community structure.					
36536283	7	85	theme	16SrRNA	1197:1203	arg1	gene					1205:1208	16SrRNA gene	1197:1208	16SrRNA gene	1197:1208	Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.					
36536283	14	86	theme	bacterial	2157:2165	arg1	overtime					2178:2185	soil bacterial population overtime	2152:2185	soil bacterial population overtime	2152:2185	CONCLUSIONS The introduction of starch-based bioplastic and oxo-LDPE led to contrasting shift in soil bacterial population overtime, which may determine their fate in the environment.					
36536283	4	87	theme	starch-based	764:775	arg1	bioplastic					777:786	biodegradable: cassava starch-based bioplastic	741:786	biodegradable: cassava starch-based bioplastic	741:786	In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE).					
35026221	0	0	theme	aqueous	107:113	arg1	solutions					115:123	aqueous solutions	107:123	aqueous solutions	107:123	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	9	1	dep	helped	1369:1374	arg1	hypothesise					1376:1386	hypothesise	1376:1386	hypothesise a mechanism for the formation of the Ch-K composite	1376:1438	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	9	2	theme	favourable	1521:1530	arg1	interactions					1547:1558	several favourable intermolecular interactions	1513:1558	several favourable intermolecular interactions	1513:1558	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	5	3	theme	D-R	822:824	arg1	model					835:839	the D-R isotherm model	818:839	the D-R isotherm model	818:839	The Langmuir model found 0.118 mol kg-1 for the maximum adsorption capacity of the Ch-K and the D-R isotherm model showed that the nature of the adsorption process was physical.					
35026221	6	4	theme	adsorption	920:929	arg1	Kinetics					904:911	Kinetics	904:911	Kinetics of the adsorption	904:929	Kinetics of the adsorption could be explained by using both IPD (intraparticle diffusion) and PSO (pseudo second order) models.					
35026221	9	5	theme	intermolecular	1532:1545	arg1	interactions					1547:1558	several favourable intermolecular interactions	1513:1558	several favourable intermolecular interactions	1513:1558	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	4	6	from	pH 7.5	709:714	arg1	500 mg L-1					695:704	500 mg L-1	695:704	500 mg L-1 at pH 7.5 at 25 °C	695:723	Adsorbent performance of the Ch-K composite for AO dye molecules was optimized: 500 mg L-1 at pH 7.5 at 25 °C.					
35026221	5	7	theme	isotherm	826:833	arg1	model					835:839	the D-R isotherm model	818:839	the D-R isotherm model	818:839	The Langmuir model found 0.118 mol kg-1 for the maximum adsorption capacity of the Ch-K and the D-R isotherm model showed that the nature of the adsorption process was physical.					
35026221	9	8	dep	M06-2X/6-31+G	1343:1355	arg1	p					1360:1360	p	1360:1360	p	1360:1360	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	9	8	dep	M06-2X/6-31+G	1343:1355	arg1	d					1358:1358	d	1358:1358	d	1358:1358	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	1	9	theme	composite	234:242	arg1	kaolin					204:209	kaolin (K, a natural mineral) composite (Ch-K)	204:249	kaolin (K, a natural mineral) composite (Ch-K)	204:249	Chitosan (Ch, a natural polymer) and kaolin (K, a natural mineral) composite (Ch-K) was produced with the help of two crosslinkers, epichlorohydrin and tripolyphosphate, and then moulded into uniform beads in tripolyphosphate solution.					
35026221	1	9	theme	composite	234:242	arg1	Ch-K					245:248	Ch-K	245:248	Ch-K	245:248	Chitosan (Ch, a natural polymer) and kaolin (K, a natural mineral) composite (Ch-K) was produced with the help of two crosslinkers, epichlorohydrin and tripolyphosphate, and then moulded into uniform beads in tripolyphosphate solution.					
35026221	0	10	link	tripolyphosphate-crosslinked	20:47	arg1	composite					65:73	tripolyphosphate-crosslinked chitosan-kaolin composite	20:73	tripolyphosphate-crosslinked chitosan-kaolin composite	20:73	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	5	11	theme	process	882:888	arg1	nature					857:862	the nature	853:862	the nature of the adsorption process	853:888	The Langmuir model found 0.118 mol kg-1 for the maximum adsorption capacity of the Ch-K and the D-R isotherm model showed that the nature of the adsorption process was physical.					
35026221	5	11	theme	process	882:888	arg1	physical					894:901	physical	894:901	physical	894:901	The Langmuir model found 0.118 mol kg-1 for the maximum adsorption capacity of the Ch-K and the D-R isotherm model showed that the nature of the adsorption process was physical.					
35026221	1	12	from	beads	367:371	arg1	solution					393:400	tripolyphosphate solution	376:400	tripolyphosphate solution	376:400	Chitosan (Ch, a natural polymer) and kaolin (K, a natural mineral) composite (Ch-K) was produced with the help of two crosslinkers, epichlorohydrin and tripolyphosphate, and then moulded into uniform beads in tripolyphosphate solution.					
35026221	8	13	dep	recovered	1187:1195	arg1	%					1200:1200	88%	1198:1200	88%	1198:1200	The activity of the composite adsorbent was recovered (88%) after the five sequential adsorption/desorption cycles.					
35026221	8	14	theme	adsorption/desorption	1229:1249	arg1	cycles					1251:1256	the five sequential adsorption/desorption cycles	1209:1256	the five sequential adsorption/desorption cycles	1209:1256	The activity of the composite adsorbent was recovered (88%) after the five sequential adsorption/desorption cycles.					
35026221	9	15	theme	M06-2X/6-31+G	1343:1355	arg1	level					1363:1367	M06-2X/6-31+G (d,p) level	1343:1367	M06-2X/6-31+G (d,p) level	1343:1367	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	7	16	theme	adsorption	1096:1105	arg1	behaviour					1079:1087	the behaviour	1075:1087	the behaviour of the adsorption	1075:1105	Thermodynamic parameters demonstrated that the behaviour of the adsorption was an endothermic and spontaneous.					
35026221	7	16	theme	adsorption	1096:1105	arg1	endothermic					1114:1124	endothermic	1114:1124	endothermic	1114:1124	Thermodynamic parameters demonstrated that the behaviour of the adsorption was an endothermic and spontaneous.					
35026221	4	17	dep	optimized	684:692	arg1	500 mg L-1					695:704	500 mg L-1	695:704	500 mg L-1 at pH 7.5 at 25 °C	695:723	Adsorbent performance of the Ch-K composite for AO dye molecules was optimized: 500 mg L-1 at pH 7.5 at 25 °C.					
35026221	8	18	theme	adsorbent	1173:1181	arg1	activity					1147:1154	The activity	1143:1154	The activity of the composite adsorbent	1143:1181	The activity of the composite adsorbent was recovered (88%) after the five sequential adsorption/desorption cycles.					
35026221	3	19	attach	removal	527:533	arg2	dye					588:590	a frequently-used industrial dye	559:590	a frequently-used industrial dye	559:590	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	3	19	attach	removal	527:533	arg2	AO					554:555	AO	554:555	AO	554:555	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	3	19	attach	removal	527:533	arg3	solutions					604:612	aqueous solutions	596:612	aqueous solutions	596:612	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	3	19	attach	removal	527:533	arg2	O					551:551	the auramine O	538:551	the auramine O (AO)	538:556	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	0	20	theme	Experimental	126:137	arg1	study					139:143	Experimental study	126:143	Experimental study	126:143	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	1	21	dep	the	269:271	arg1	help					273:276	help	273:276	help	273:276	Chitosan (Ch, a natural polymer) and kaolin (K, a natural mineral) composite (Ch-K) was produced with the help of two crosslinkers, epichlorohydrin and tripolyphosphate, and then moulded into uniform beads in tripolyphosphate solution.					
35026221	7	22	theme	Thermodynamic	1032:1044	arg1	parameters					1046:1055	Thermodynamic parameters	1032:1055	Thermodynamic parameters	1032:1055	Thermodynamic parameters demonstrated that the behaviour of the adsorption was an endothermic and spontaneous.					
35026221	6	23	theme	PSO	998:1000	arg1	models					1024:1029	both IPD (intraparticle diffusion) and PSO (pseudo second order) models	959:1029	both IPD (intraparticle diffusion) and PSO (pseudo second order) models	959:1029	Kinetics of the adsorption could be explained by using both IPD (intraparticle diffusion) and PSO (pseudo second order) models.					
35026221	3	24	used	used	493:496	arg2	beads					477:481	The beads	473:481	The beads	473:481	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	3	24	used	used	493:496	arg2	adsorbent					513:521	adsorbent	513:521	adsorbent	513:521	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	5	25	theme	Langmuir	730:737	arg1	model					739:743	The Langmuir model	726:743	The Langmuir model	726:743	The Langmuir model found 0.118 mol kg-1 for the maximum adsorption capacity of the Ch-K and the D-R isotherm model showed that the nature of the adsorption process was physical.					
35026221	3	26	theme	aqueous	596:602	arg1	solutions					604:612	aqueous solutions	596:612	aqueous solutions	596:612	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	4	27	theme	dye	666:668	arg1	molecules					670:678	AO dye molecules	663:678	AO dye molecules	663:678	Adsorbent performance of the Ch-K composite for AO dye molecules was optimized: 500 mg L-1 at pH 7.5 at 25 °C.					
35026221	5	28	theme	Ch-K	809:812	arg1	capacity					793:800	the maximum adsorption capacity	770:800	the maximum adsorption capacity of the Ch-K	770:812	The Langmuir model found 0.118 mol kg-1 for the maximum adsorption capacity of the Ch-K and the D-R isotherm model showed that the nature of the adsorption process was physical.					
35026221	0	29	theme	chitosan-kaolin	49:63	arg1	composite					65:73	tripolyphosphate-crosslinked chitosan-kaolin composite	20:73	tripolyphosphate-crosslinked chitosan-kaolin composite	20:73	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	0	30	theme	DFT	149:151	arg1	calculations					153:164	DFT calculations	149:164	DFT calculations	149:164	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	9	31	theme	AO	1494:1495	arg1	dye					1497:1499	AO dye	1494:1499	AO dye	1494:1499	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	1	32	dep	Chitosan	167:174	arg1	polymer					191:197	a natural polymer	181:197	a natural polymer	181:197	Chitosan (Ch, a natural polymer) and kaolin (K, a natural mineral) composite (Ch-K) was produced with the help of two crosslinkers, epichlorohydrin and tripolyphosphate, and then moulded into uniform beads in tripolyphosphate solution.					
35026221	1	32	dep	Chitosan	167:174	arg1	Ch					177:178	Ch	177:178	Ch	177:178	Chitosan (Ch, a natural polymer) and kaolin (K, a natural mineral) composite (Ch-K) was produced with the help of two crosslinkers, epichlorohydrin and tripolyphosphate, and then moulded into uniform beads in tripolyphosphate solution.					
35026221	9	33	theme	adsorption	1470:1479	arg1	behaviour					1481:1489	the adsorption behaviour	1466:1489	the adsorption behaviour of AO dye	1466:1499	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	0	34	theme	tripolyphosphate-crosslinked	20:47	arg1	composite					65:73	tripolyphosphate-crosslinked chitosan-kaolin composite	20:73	tripolyphosphate-crosslinked chitosan-kaolin composite	20:73	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	5	35	theme	adsorption	871:880	arg1	process					882:888	the adsorption process	867:888	the adsorption process	867:888	The Langmuir model found 0.118 mol kg-1 for the maximum adsorption capacity of the Ch-K and the D-R isotherm model showed that the nature of the adsorption process was physical.					
35026221	9	36	theme	Ch-K	1425:1428	arg1	composite					1430:1438	the Ch-K composite	1421:1438	the Ch-K composite	1421:1438	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	6	37	theme	IPD	964:966	arg1	models					1024:1029	both IPD (intraparticle diffusion) and PSO (pseudo second order) models	959:1029	both IPD (intraparticle diffusion) and PSO (pseudo second order) models	959:1029	Kinetics of the adsorption could be explained by using both IPD (intraparticle diffusion) and PSO (pseudo second order) models.					
35026221	4	38	theme	Ch-K	644:647	arg1	composite					649:657	the Ch-K composite	640:657	the Ch-K composite	640:657	Adsorbent performance of the Ch-K composite for AO dye molecules was optimized: 500 mg L-1 at pH 7.5 at 25 °C.					
35026221	9	39	dep	in	1321:1322	arg1	silico					1324:1329	silico	1324:1329	silico	1324:1329	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	3	40	theme	O	551:551	arg1	removal					527:533	removal	527:533	removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions	527:612	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	6	41	theme	intraparticle	969:981	arg1	IPD					964:966	IPD	964:966	IPD (intraparticle diffusion)	964:992	Kinetics of the adsorption could be explained by using both IPD (intraparticle diffusion) and PSO (pseudo second order) models.					
35026221	6	41	theme	intraparticle	969:981	arg1	diffusion					983:991	intraparticle diffusion	969:991	intraparticle diffusion	969:991	Kinetics of the adsorption could be explained by using both IPD (intraparticle diffusion) and PSO (pseudo second order) models.					
35026221	5	42	theme	maximum	774:780	arg1	capacity					793:800	the maximum adsorption capacity	770:800	the maximum adsorption capacity of the Ch-K	770:812	The Langmuir model found 0.118 mol kg-1 for the maximum adsorption capacity of the Ch-K and the D-R isotherm model showed that the nature of the adsorption process was physical.					
35026221	9	43	theme	experimental	1272:1283	arg1	findings					1285:1292	experimental findings	1272:1292	experimental findings	1272:1292	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	1	44	theme	uniform	359:365	arg1	beads					367:371	uniform beads	359:371	uniform beads in tripolyphosphate solution	359:400	Chitosan (Ch, a natural polymer) and kaolin (K, a natural mineral) composite (Ch-K) was produced with the help of two crosslinkers, epichlorohydrin and tripolyphosphate, and then moulded into uniform beads in tripolyphosphate solution.					
35026221	5	45	theme	adsorption	782:791	arg1	capacity					793:800	the maximum adsorption capacity	770:800	the maximum adsorption capacity of the Ch-K	770:812	The Langmuir model found 0.118 mol kg-1 for the maximum adsorption capacity of the Ch-K and the D-R isotherm model showed that the nature of the adsorption process was physical.					
35026221	0	46	theme	O	88:88	arg1	removal					94:100	Auramine O dye removal	79:100	Auramine O dye removal	79:100	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	3	47	theme	industrial	577:586	arg1	dye					588:590	a frequently-used industrial dye	559:590	a frequently-used industrial dye	559:590	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	3	47	theme	industrial	577:586	arg1	O					551:551	the auramine O	538:551	the auramine O (AO)	538:556	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	4	48	theme	composite	649:657	arg1	performance					625:635	Adsorbent performance	615:635	Adsorbent performance of the Ch-K composite for AO dye molecules	615:678	Adsorbent performance of the Ch-K composite for AO dye molecules was optimized: 500 mg L-1 at pH 7.5 at 25 °C.					
35026221	0	49	theme	Auramine	79:86	arg1	removal					94:100	Auramine O dye removal	79:100	Auramine O dye removal	79:100	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	8	50	theme	sequential	1218:1227	arg1	cycles					1251:1256	the five sequential adsorption/desorption cycles	1209:1256	the five sequential adsorption/desorption cycles	1209:1256	The activity of the composite adsorbent was recovered (88%) after the five sequential adsorption/desorption cycles.					
35026221	3	51	theme	auramine	542:549	arg1	O					551:551	the auramine O	538:551	the auramine O (AO)	538:556	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	3	51	theme	auramine	542:549	arg1	dye					588:590	a frequently-used industrial dye	559:590	a frequently-used industrial dye	559:590	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	3	51	theme	auramine	542:549	arg1	AO					554:555	AO	554:555	AO	554:555	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	3	52	theme	frequently-used	561:575	arg1	dye					588:590	a frequently-used industrial dye	559:590	a frequently-used industrial dye	559:590	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	3	52	theme	frequently-used	561:575	arg1	O					551:551	the auramine O	538:551	the auramine O (AO)	538:556	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	0	53	dep	Epichlorohydrin	0:14	arg1	calculations					153:164	DFT calculations	149:164	DFT calculations	149:164	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	0	53	dep	Epichlorohydrin	0:14	arg1	study					139:143	Experimental study	126:143	Experimental study	126:143	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	1	54	theme	tripolyphosphate	376:391	arg1	solution					393:400	tripolyphosphate solution	376:400	tripolyphosphate solution	376:400	Chitosan (Ch, a natural polymer) and kaolin (K, a natural mineral) composite (Ch-K) was produced with the help of two crosslinkers, epichlorohydrin and tripolyphosphate, and then moulded into uniform beads in tripolyphosphate solution.					
35026221	9	55	theme	dye	1497:1499	arg1	behaviour					1481:1489	the adsorption behaviour	1466:1489	the adsorption behaviour of AO dye	1466:1499	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	9	56	theme	several	1513:1519	arg1	interactions					1547:1558	several favourable intermolecular interactions	1513:1558	several favourable intermolecular interactions	1513:1558	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	4	57	theme	AO	663:664	arg1	molecules					670:678	AO dye molecules	663:678	AO dye molecules	663:678	Adsorbent performance of the Ch-K composite for AO dye molecules was optimized: 500 mg L-1 at pH 7.5 at 25 °C.					
35026221	9	58	theme	composite	1430:1438	arg1	formation					1408:1416	the formation	1404:1416	the formation of the Ch-K composite	1404:1438	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35026221	6	59	theme	pseudo	1003:1008	arg1	PSO					998:1000	PSO	998:1000	PSO (pseudo second order)	998:1022	Kinetics of the adsorption could be explained by using both IPD (intraparticle diffusion) and PSO (pseudo second order) models.					
35026221	6	59	theme	pseudo	1003:1008	arg1	order					1017:1021	pseudo second order	1003:1021	pseudo second order	1003:1021	Kinetics of the adsorption could be explained by using both IPD (intraparticle diffusion) and PSO (pseudo second order) models.					
35026221	3	60	from	removal	527:533	arg1	solutions					604:612	aqueous solutions	596:612	aqueous solutions	596:612	The beads were then used as the natural adsorbent for removal of the auramine O (AO), a frequently-used industrial dye, in aqueous solutions.					
35026221	0	61	from	solutions	115:123	arg1	Epichlorohydrin					0:14	Epichlorohydrin	0:14	Epichlorohydrin	0:14	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	0	61	from	solutions	115:123	arg1	composite					65:73	tripolyphosphate-crosslinked chitosan-kaolin composite	20:73	tripolyphosphate-crosslinked chitosan-kaolin composite	20:73	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	6	62	theme	second	1010:1015	arg1	PSO					998:1000	PSO	998:1000	PSO (pseudo second order)	998:1022	Kinetics of the adsorption could be explained by using both IPD (intraparticle diffusion) and PSO (pseudo second order) models.					
35026221	6	62	theme	second	1010:1015	arg1	order					1017:1021	pseudo second order	1003:1021	pseudo second order	1003:1021	Kinetics of the adsorption could be explained by using both IPD (intraparticle diffusion) and PSO (pseudo second order) models.					
35026221	1	63	theme	natural	183:189	arg1	polymer					191:197	a natural polymer	181:197	a natural polymer	181:197	Chitosan (Ch, a natural polymer) and kaolin (K, a natural mineral) composite (Ch-K) was produced with the help of two crosslinkers, epichlorohydrin and tripolyphosphate, and then moulded into uniform beads in tripolyphosphate solution.					
35026221	1	63	theme	natural	183:189	arg1	Ch					177:178	Ch	177:178	Ch	177:178	Chitosan (Ch, a natural polymer) and kaolin (K, a natural mineral) composite (Ch-K) was produced with the help of two crosslinkers, epichlorohydrin and tripolyphosphate, and then moulded into uniform beads in tripolyphosphate solution.					
35026221	0	64	theme	dye	90:92	arg1	removal					94:100	Auramine O dye removal	79:100	Auramine O dye removal	79:100	Epichlorohydrin and tripolyphosphate-crosslinked chitosan-kaolin composite for Auramine O dye removal from aqueous solutions: Experimental study and DFT calculations.					
35026221	1	65	dep	kaolin	204:209	arg1	mineral					225:231	mineral	225:231	mineral	225:231	Chitosan (Ch, a natural polymer) and kaolin (K, a natural mineral) composite (Ch-K) was produced with the help of two crosslinkers, epichlorohydrin and tripolyphosphate, and then moulded into uniform beads in tripolyphosphate solution.					
35026221	1	65	dep	kaolin	204:209	arg1	K					212:212	K	212:212	K	212:212	Chitosan (Ch, a natural polymer) and kaolin (K, a natural mineral) composite (Ch-K) was produced with the help of two crosslinkers, epichlorohydrin and tripolyphosphate, and then moulded into uniform beads in tripolyphosphate solution.					
35026221	4	66	from	25 °C	719:723	arg1	500 mg L-1					695:704	500 mg L-1	695:704	500 mg L-1 at pH 7.5 at 25 °C	695:723	Adsorbent performance of the Ch-K composite for AO dye molecules was optimized: 500 mg L-1 at pH 7.5 at 25 °C.					
35026221	4	67	theme	Adsorbent	615:623	arg1	performance					625:635	Adsorbent performance	615:635	Adsorbent performance of the Ch-K composite for AO dye molecules	615:678	Adsorbent performance of the Ch-K composite for AO dye molecules was optimized: 500 mg L-1 at pH 7.5 at 25 °C.					
35026221	9	68	theme	in	1321:1322	arg1	modeling					1331:1338	in silico modeling	1321:1338	in silico modeling	1321:1338	Supported by experimental findings, the results obtained from in silico modeling at M06-2X/6-31+G (d,p) level helped hypothesise a mechanism for the formation of the Ch-K composite, and shed some light onto the adsorption behaviour of AO dye by assuming several favourable intermolecular interactions.					
35712026	8	0	theme	allotype	1239:1246	arg1	sites					1199:1203	All five sites	1190:1203	All five sites of the tighter-binding CD16a V158 allotype	1190:1246	All five sites of the tighter-binding CD16a V158 allotype showed 65-100% N-glycan occupancy in proteomics-based experiments.					
35712026	8	0	theme	allotype	1239:1246	arg1	allotype					1239:1246	the tighter-binding CD16a V158 allotype	1208:1246	the tighter-binding CD16a V158 allotype	1208:1246	All five sites of the tighter-binding CD16a V158 allotype showed 65-100% N-glycan occupancy in proteomics-based experiments.					
35712026	6	1	dep	low	1006:1008	arg1	to					1010:1011	to	1010:1011	to	1010:1011	The level of N-glycosylation at each site is not known though computational prediction indicates low to moderate potential at each site based on primary sequence (40-70%).					
35712026	6	1	dep	low	1006:1008	arg1	moderate					1013:1020	moderate	1013:1020	moderate	1013:1020	The level of N-glycosylation at each site is not known though computational prediction indicates low to moderate potential at each site based on primary sequence (40-70%).					
35712026	10	2	theme	natural	1677:1683	arg1	cells					1692:1696	cultured human natural killer cells	1662:1696	cultured human natural killer cells	1662:1696	Occupancy was likewise high for the membrane-bound receptor at all detected N-glycosylation sites using CD16a purified from cultured human natural killer cells.					
35712026	3	3	theme	CD16a-mediated	527:540	arg1	responses					568:576	CD16a-mediated antibody-dependent immune responses	527:576	CD16a-mediated antibody-dependent immune responses	527:576	Currently, affinities are measured using recombinantly-expressed soluble extracellular FcγR domains and CD16a-mediated antibody-dependent immune responses are characterized using cultured cells.					
35712026	8	4	theme	65-100	1255:1260	arg1	%					1261:1261	%	1261:1261	%	1261:1261	All five sites of the tighter-binding CD16a V158 allotype showed 65-100% N-glycan occupancy in proteomics-based experiments.					
35712026	0	5	with	N-glycosylated	55:68	arg1	occupancy					80:88	high occupancy	75:88	high occupancy at all five sites	75:106	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.					
35712026	1	6	theme	full	261:264	arg1	effect					266:271	full effect	261:271	full effect	261:271	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	8	7	theme	N-glycan	1263:1270	arg1	occupancy					1272:1280	65-100% N-glycan occupancy	1255:1280	65-100% N-glycan occupancy	1255:1280	All five sites of the tighter-binding CD16a V158 allotype showed 65-100% N-glycan occupancy in proteomics-based experiments.					
35712026	6	8	theme	computational	971:983	arg1	prediction					985:994	computational prediction	971:994	computational prediction	971:994	The level of N-glycosylation at each site is not known though computational prediction indicates low to moderate potential at each site based on primary sequence (40-70%).					
35712026	4	9	gly	N-glycosylation	677:691	arg2	sites					693:697	multiple N-glycosylation sites	668:697	multiple N-glycosylation sites	668:697	It is notable that CD16a is highly processed with multiple N-glycosylation sites, and preventing individual N-glycan modifications affects affinity.					
35712026	12	10	theme	F158	1869:1872	arg1	allotype					1874:1881	The weaker-binding CD16a F158 allotype	1844:1881	The weaker-binding CD16a F158 allotype	1844:1881	The weaker-binding CD16a F158 allotype showed higher occupancy of >93% at each site.					
35712026	4	11	theme	N-glycan	726:733	arg1	modifications					735:747	individual N-glycan modifications	715:747	individual N-glycan modifications	715:747	It is notable that CD16a is highly processed with multiple N-glycosylation sites, and preventing individual N-glycan modifications affects affinity.					
35712026	1	12	theme	life-saving	161:171	arg1	responses					180:188	life-saving immune responses	161:188	life-saving immune responses	161:188	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	6	13	theme	primary	1054:1060	arg1	%					1077:1077	40-70%	1072:1077	40-70%	1072:1077	The level of N-glycosylation at each site is not known though computational prediction indicates low to moderate potential at each site based on primary sequence (40-70%).					
35712026	6	13	theme	primary	1054:1060	arg1	sequence					1062:1069	primary sequence	1054:1069	primary sequence (40-70%)	1054:1078	The level of N-glycosylation at each site is not known though computational prediction indicates low to moderate potential at each site based on primary sequence (40-70%).					
35712026	4	14	theme	N-glycosylation	677:691	arg1	sites					693:697	multiple N-glycosylation sites	668:697	multiple N-glycosylation sites	668:697	It is notable that CD16a is highly processed with multiple N-glycosylation sites, and preventing individual N-glycan modifications affects affinity.					
35712026	9	15	theme	intact	1355:1360	arg1	spectrometry					1375:1386	intact protein mass spectrometry	1355:1386	intact protein mass spectrometry that demonstrated the predominant species corresponded to CD16a V158 with five N-glycans, with a smaller contribution from CD16a with four N-glycans	1355:1535	These observations were confirmed using intact protein mass spectrometry that demonstrated the predominant species corresponded to CD16a V158 with five N-glycans, with a smaller contribution from CD16a with four N-glycans.					
35712026	1	16	theme	many	194:197	arg1	antibodies					222:231	many therapeutic monoclonal antibodies	194:231	many therapeutic monoclonal antibodies	194:231	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	7	17	theme	extracellular	1115:1127	arg1	domains					1129:1135	the extracellular domains	1111:1135	the extracellular domains using complementary mass spectrometry-based methods	1111:1187	Here we quantify occupancy of the extracellular domains using complementary mass spectrometry-based methods.					
35712026	9	18	theme	mass	1370:1373	arg1	spectrometry					1375:1386	intact protein mass spectrometry	1355:1386	intact protein mass spectrometry that demonstrated the predominant species corresponded to CD16a V158 with five N-glycans, with a smaller contribution from CD16a with four N-glycans	1355:1535	These observations were confirmed using intact protein mass spectrometry that demonstrated the predominant species corresponded to CD16a V158 with five N-glycans, with a smaller contribution from CD16a with four N-glycans.					
35712026	1	19	theme	antibody-binding	113:128	arg1	FcγRs					146:150	FcγRs	146:150	FcγRs	146:150	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	1	19	theme	antibody-binding	113:128	arg1	receptors					135:143	The antibody-binding Fc γ receptors	109:143	The antibody-binding Fc γ receptors (FcγRs)	109:151	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	12	20	theme	CD16a	1863:1867	arg1	allotype					1874:1881	The weaker-binding CD16a F158 allotype	1844:1881	The weaker-binding CD16a F158 allotype	1844:1881	The weaker-binding CD16a F158 allotype showed higher occupancy of >93% at each site.					
35712026	1	21	theme	γ	133:133	arg1	FcγRs					146:150	FcγRs	146:150	FcγRs	146:150	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	1	21	theme	γ	133:133	arg1	receptors					135:143	The antibody-binding Fc γ receptors	109:143	The antibody-binding Fc γ receptors (FcγRs)	109:151	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	10	22	theme	membrane-bound	1574:1587	arg1	receptor					1589:1596	the membrane-bound receptor	1570:1596	the membrane-bound receptor at all detected N-glycosylation sites	1570:1634	Occupancy was likewise high for the membrane-bound receptor at all detected N-glycosylation sites using CD16a purified from cultured human natural killer cells.					
35712026	0	23	gly	N-glycosylated	55:68	arg1	IIIa					39:42	The antibody-binding Fc gamma receptor IIIa	0:42	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.	0:107	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.					
35712026	0	23	gly	N-glycosylated	55:68	arg1	CD16a					46:50	CD16a	46:50	CD16a	46:50	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.					
35712026	10	24	theme	detected	1605:1612	arg1	sites					1630:1634	all detected N-glycosylation sites	1601:1634	all detected N-glycosylation sites	1601:1634	Occupancy was likewise high for the membrane-bound receptor at all detected N-glycosylation sites using CD16a purified from cultured human natural killer cells.					
35712026	5	25	theme	CD16a	819:823	arg1	composition					834:844	CD16a N-glycan composition	819:844	CD16a N-glycan composition	819:844	Furthermore, multiple groups have demonstrated that CD16a N-glycan composition is variable and composition impacts antibody binding affinity.					
35712026	0	26	theme	Fc	21:22	arg1	IIIa					39:42	The antibody-binding Fc gamma receptor IIIa	0:42	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.	0:107	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.					
35712026	11	27	theme	protein	1835:1841	arg1	analysis					1806:1813	analysis	1806:1813	analysis of the T171A mutant protein	1806:1841	Occupancy of the N162 site, critical for antibody binding, appeared independent of N169 occupancy based on analysis of the T171A mutant protein.					
35712026	0	28	theme	receptor	30:37	arg1	IIIa					39:42	The antibody-binding Fc gamma receptor IIIa	0:42	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.	0:107	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.					
35712026	10	29	theme	human	1671:1675	arg1	cells					1692:1696	cultured human natural killer cells	1662:1696	cultured human natural killer cells	1662:1696	Occupancy was likewise high for the membrane-bound receptor at all detected N-glycosylation sites using CD16a purified from cultured human natural killer cells.					
35712026	11	30	theme	T171A	1822:1826	arg1	protein					1835:1841	the T171A mutant protein	1818:1841	the T171A mutant protein	1818:1841	Occupancy of the N162 site, critical for antibody binding, appeared independent of N169 occupancy based on analysis of the T171A mutant protein.					
35712026	3	31	theme	FcγR	510:513	arg1	domains					515:521	recombinantly-expressed soluble extracellular FcγR domains	464:521	recombinantly-expressed soluble extracellular FcγR domains	464:521	Currently, affinities are measured using recombinantly-expressed soluble extracellular FcγR domains and CD16a-mediated antibody-dependent immune responses are characterized using cultured cells.					
35712026	5	32	theme	antibody	882:889	arg1	affinity					899:906	antibody binding affinity	882:906	antibody binding affinity	882:906	Furthermore, multiple groups have demonstrated that CD16a N-glycan composition is variable and composition impacts antibody binding affinity.					
35712026	3	33	theme	soluble	488:494	arg1	domains					515:521	recombinantly-expressed soluble extracellular FcγR domains	464:521	recombinantly-expressed soluble extracellular FcγR domains	464:521	Currently, affinities are measured using recombinantly-expressed soluble extracellular FcγR domains and CD16a-mediated antibody-dependent immune responses are characterized using cultured cells.					
35712026	1	34	theme	therapeutic	199:209	arg1	antibodies					222:231	many therapeutic monoclonal antibodies	194:231	many therapeutic monoclonal antibodies	194:231	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	3	35	theme	antibody-dependent	542:559	arg1	responses					568:576	CD16a-mediated antibody-dependent immune responses	527:576	CD16a-mediated antibody-dependent immune responses	527:576	Currently, affinities are measured using recombinantly-expressed soluble extracellular FcγR domains and CD16a-mediated antibody-dependent immune responses are characterized using cultured cells.					
35712026	8	36	theme	tighter-binding	1212:1226	arg1	allotype					1239:1246	the tighter-binding CD16a V158 allotype	1208:1246	the tighter-binding CD16a V158 allotype	1208:1246	All five sites of the tighter-binding CD16a V158 allotype showed 65-100% N-glycan occupancy in proteomics-based experiments.					
35712026	12	37	gly	occupancy	1897:1905	arg2	%					1913:1913	>93%	1910:1913	>93%	1910:1913	The weaker-binding CD16a F158 allotype showed higher occupancy of >93% at each site.					
35712026	0	38	from	sites	102:106	arg1	occupancy					80:88	high occupancy	75:88	high occupancy at all five sites	75:106	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.					
35712026	10	39	theme	killer	1685:1690	arg1	cells					1692:1696	cultured human natural killer cells	1662:1696	cultured human natural killer cells	1662:1696	Occupancy was likewise high for the membrane-bound receptor at all detected N-glycosylation sites using CD16a purified from cultured human natural killer cells.					
35712026	11	40	theme	occupancy	1787:1795	arg1	independent					1767:1777	independent	1767:1777	independent	1767:1777	Occupancy of the N162 site, critical for antibody binding, appeared independent of N169 occupancy based on analysis of the T171A mutant protein.					
35712026	1	41	theme	FcγR	241:244	arg1	engagement					246:255	FcγR engagement	241:255	FcγR engagement	241:255	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	8	42	theme	V158	1234:1237	arg1	allotype					1239:1246	the tighter-binding CD16a V158 allotype	1208:1246	the tighter-binding CD16a V158 allotype	1208:1246	All five sites of the tighter-binding CD16a V158 allotype showed 65-100% N-glycan occupancy in proteomics-based experiments.					
35712026	6	43	theme	low	1006:1008	arg1	potential					1022:1030	low to moderate potential	1006:1030	low to moderate potential	1006:1030	The level of N-glycosylation at each site is not known though computational prediction indicates low to moderate potential at each site based on primary sequence (40-70%).					
35712026	9	44	dep	demonstrated	1393:1404	arg1	corresponded					1430:1441	corresponded	1430:1441	corresponded	1430:1441	These observations were confirmed using intact protein mass spectrometry that demonstrated the predominant species corresponded to CD16a V158 with five N-glycans, with a smaller contribution from CD16a with four N-glycans.					
35712026	2	45	theme	proven	278:283	arg1	strategy					285:292	One proven strategy	274:292	One proven strategy to improve the efficacy of antibody therapies	274:338	One proven strategy to improve the efficacy of antibody therapies is to increase receptor binding affinity, in particular binding to FcγRIIIa/CD16a.					
35712026	12	46	theme	%	1913:1913	arg1	occupancy					1897:1905	higher occupancy	1890:1905	higher occupancy of >93%	1890:1913	The weaker-binding CD16a F158 allotype showed higher occupancy of >93% at each site.					
35712026	2	47	theme	therapies	330:338	arg1	efficacy					309:316	the efficacy	305:316	the efficacy of antibody therapies	305:338	One proven strategy to improve the efficacy of antibody therapies is to increase receptor binding affinity, in particular binding to FcγRIIIa/CD16a.					
35712026	8	48	theme	%	1261:1261	arg1	occupancy					1272:1280	65-100% N-glycan occupancy	1255:1280	65-100% N-glycan occupancy	1255:1280	All five sites of the tighter-binding CD16a V158 allotype showed 65-100% N-glycan occupancy in proteomics-based experiments.					
35712026	6	49	from	site	946:949	arg1	level					913:917	The level	909:917	The level of N-glycosylation at each site	909:949	The level of N-glycosylation at each site is not known though computational prediction indicates low to moderate potential at each site based on primary sequence (40-70%).					
35712026	7	50	theme	spectrometry-based	1162:1179	arg1	methods					1181:1187	complementary mass spectrometry-based methods	1143:1187	complementary mass spectrometry-based methods	1143:1187	Here we quantify occupancy of the extracellular domains using complementary mass spectrometry-based methods.					
35712026	11	51	theme	N162	1716:1719	arg1	site					1721:1724	the N162 site	1712:1724	the N162 site	1712:1724	Occupancy of the N162 site, critical for antibody binding, appeared independent of N169 occupancy based on analysis of the T171A mutant protein.					
35712026	12	52	theme	higher	1890:1895	arg1	occupancy					1897:1905	higher occupancy	1890:1905	higher occupancy of >93%	1890:1913	The weaker-binding CD16a F158 allotype showed higher occupancy of >93% at each site.					
35712026	4	53	theme	individual	715:724	arg1	modifications					735:747	individual N-glycan modifications	715:747	individual N-glycan modifications	715:747	It is notable that CD16a is highly processed with multiple N-glycosylation sites, and preventing individual N-glycan modifications affects affinity.					
35712026	7	54	theme	complementary	1143:1155	arg1	methods					1181:1187	complementary mass spectrometry-based methods	1143:1187	complementary mass spectrometry-based methods	1143:1187	Here we quantify occupancy of the extracellular domains using complementary mass spectrometry-based methods.					
35712026	3	55	theme	cultured	602:609	arg1	cells					611:615	cultured cells	602:615	cultured cells	602:615	Currently, affinities are measured using recombinantly-expressed soluble extracellular FcγR domains and CD16a-mediated antibody-dependent immune responses are characterized using cultured cells.					
35712026	1	56	theme	immune	173:178	arg1	responses					180:188	life-saving immune responses	161:188	life-saving immune responses	161:188	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	8	57	theme	proteomics-based	1285:1300	arg1	experiments					1302:1312	proteomics-based experiments	1285:1312	proteomics-based experiments	1285:1312	All five sites of the tighter-binding CD16a V158 allotype showed 65-100% N-glycan occupancy in proteomics-based experiments.					
35712026	2	58	theme	receptor	355:362	arg1	affinity					372:379	receptor binding affinity	355:379	receptor binding affinity	355:379	One proven strategy to improve the efficacy of antibody therapies is to increase receptor binding affinity, in particular binding to FcγRIIIa/CD16a.					
35712026	10	59	gly	N-glycosylation	1614:1628	arg2	sites					1630:1634	all detected N-glycosylation sites	1601:1634	all detected N-glycosylation sites	1601:1634	Occupancy was likewise high for the membrane-bound receptor at all detected N-glycosylation sites using CD16a purified from cultured human natural killer cells.					
35712026	4	60	theme	multiple	668:675	arg1	sites					693:697	multiple N-glycosylation sites	668:697	multiple N-glycosylation sites	668:697	It is notable that CD16a is highly processed with multiple N-glycosylation sites, and preventing individual N-glycan modifications affects affinity.					
35712026	7	61	theme	domains	1129:1135	arg1	occupancy					1098:1106	occupancy	1098:1106	occupancy of the extracellular domains using complementary mass spectrometry-based methods	1098:1187	Here we quantify occupancy of the extracellular domains using complementary mass spectrometry-based methods.					
35712026	9	62	theme	protein	1362:1368	arg1	spectrometry					1375:1386	intact protein mass spectrometry	1355:1386	intact protein mass spectrometry that demonstrated the predominant species corresponded to CD16a V158 with five N-glycans, with a smaller contribution from CD16a with four N-glycans	1355:1535	These observations were confirmed using intact protein mass spectrometry that demonstrated the predominant species corresponded to CD16a V158 with five N-glycans, with a smaller contribution from CD16a with four N-glycans.					
35712026	2	63	dep	strategy	285:292	arg1	improve					297:303	improve	297:303	to improve the efficacy of antibody therapies	294:338	One proven strategy to improve the efficacy of antibody therapies is to increase receptor binding affinity, in particular binding to FcγRIIIa/CD16a.					
35712026	10	64	from	sites	1630:1634	arg1	receptor					1589:1596	the membrane-bound receptor	1570:1596	the membrane-bound receptor at all detected N-glycosylation sites	1570:1634	Occupancy was likewise high for the membrane-bound receptor at all detected N-glycosylation sites using CD16a purified from cultured human natural killer cells.					
35712026	7	65	gly	occupancy	1098:1106	arg2	domains					1129:1135	the extracellular domains	1111:1135	the extracellular domains using complementary mass spectrometry-based methods	1111:1187	Here we quantify occupancy of the extracellular domains using complementary mass spectrometry-based methods.					
35712026	5	66	theme	N-glycan	825:832	arg1	composition					834:844	CD16a N-glycan composition	819:844	CD16a N-glycan composition	819:844	Furthermore, multiple groups have demonstrated that CD16a N-glycan composition is variable and composition impacts antibody binding affinity.					
35712026	1	67	theme	Fc	130:131	arg1	FcγRs					146:150	FcγRs	146:150	FcγRs	146:150	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	1	67	theme	Fc	130:131	arg1	receptors					135:143	The antibody-binding Fc γ receptors	109:143	The antibody-binding Fc γ receptors (FcγRs)	109:151	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	12	68	theme	weaker-binding	1848:1861	arg1	allotype					1874:1881	The weaker-binding CD16a F158 allotype	1844:1881	The weaker-binding CD16a F158 allotype	1844:1881	The weaker-binding CD16a F158 allotype showed higher occupancy of >93% at each site.					
35712026	0	69	theme	high	75:78	arg1	occupancy					80:88	high occupancy	75:88	high occupancy at all five sites	75:106	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.					
35712026	9	70	theme	predominant	1410:1420	arg1	species					1422:1428	the predominant species	1406:1428	the predominant species	1406:1428	These observations were confirmed using intact protein mass spectrometry that demonstrated the predominant species corresponded to CD16a V158 with five N-glycans, with a smaller contribution from CD16a with four N-glycans.					
35712026	3	71	theme	extracellular	496:508	arg1	domains					515:521	recombinantly-expressed soluble extracellular FcγR domains	464:521	recombinantly-expressed soluble extracellular FcγR domains	464:521	Currently, affinities are measured using recombinantly-expressed soluble extracellular FcγR domains and CD16a-mediated antibody-dependent immune responses are characterized using cultured cells.					
35712026	9	72	theme	CD16a	1446:1450	arg1	V158					1452:1455	CD16a V158	1446:1455	CD16a V158	1446:1455	These observations were confirmed using intact protein mass spectrometry that demonstrated the predominant species corresponded to CD16a V158 with five N-glycans, with a smaller contribution from CD16a with four N-glycans.					
35712026	5	73	theme	binding	891:897	arg1	affinity					899:906	antibody binding affinity	882:906	antibody binding affinity	882:906	Furthermore, multiple groups have demonstrated that CD16a N-glycan composition is variable and composition impacts antibody binding affinity.					
35712026	5	74	theme	multiple	780:787	arg1	groups					789:794	multiple groups	780:794	multiple groups	780:794	Furthermore, multiple groups have demonstrated that CD16a N-glycan composition is variable and composition impacts antibody binding affinity.					
35712026	2	75	theme	binding	364:370	arg1	affinity					372:379	receptor binding affinity	355:379	receptor binding affinity	355:379	One proven strategy to improve the efficacy of antibody therapies is to increase receptor binding affinity, in particular binding to FcγRIIIa/CD16a.					
35712026	0	76	theme	gamma	24:28	arg1	IIIa					39:42	The antibody-binding Fc gamma receptor IIIa	0:42	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.	0:107	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.					
35712026	11	77	theme	mutant	1828:1833	arg1	protein					1835:1841	the T171A mutant protein	1818:1841	the T171A mutant protein	1818:1841	Occupancy of the N162 site, critical for antibody binding, appeared independent of N169 occupancy based on analysis of the T171A mutant protein.					
35712026	3	78	theme	recombinantly-expressed	464:486	arg1	domains					515:521	recombinantly-expressed soluble extracellular FcγR domains	464:521	recombinantly-expressed soluble extracellular FcγR domains	464:521	Currently, affinities are measured using recombinantly-expressed soluble extracellular FcγR domains and CD16a-mediated antibody-dependent immune responses are characterized using cultured cells.					
35712026	10	79	theme	N-glycosylation	1614:1628	arg1	sites					1630:1634	all detected N-glycosylation sites	1601:1634	all detected N-glycosylation sites	1601:1634	Occupancy was likewise high for the membrane-bound receptor at all detected N-glycosylation sites using CD16a purified from cultured human natural killer cells.					
35712026	11	80	theme	critical	1727:1734	arg1	site					1721:1724	the N162 site	1712:1724	the N162 site	1712:1724	Occupancy of the N162 site, critical for antibody binding, appeared independent of N169 occupancy based on analysis of the T171A mutant protein.					
35712026	2	81	theme	particular	385:394	arg1	binding					396:402	particular binding	385:402	particular binding to FcγRIIIa/CD16a	385:420	One proven strategy to improve the efficacy of antibody therapies is to increase receptor binding affinity, in particular binding to FcγRIIIa/CD16a.					
35712026	9	82	theme	smaller	1485:1491	arg1	contribution					1493:1504	a smaller contribution	1483:1504	a smaller contribution from CD16a with four N-glycans	1483:1535	These observations were confirmed using intact protein mass spectrometry that demonstrated the predominant species corresponded to CD16a V158 with five N-glycans, with a smaller contribution from CD16a with four N-glycans.					
35712026	0	83	dep	IIIa	39:42	arg1	N-glycosylated					55:68	N-glycosylated	55:68	N-glycosylated	55:68	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.					
35712026	10	84	theme	cultured	1662:1669	arg1	cells					1692:1696	cultured human natural killer cells	1662:1696	cultured human natural killer cells	1662:1696	Occupancy was likewise high for the membrane-bound receptor at all detected N-glycosylation sites using CD16a purified from cultured human natural killer cells.					
35712026	11	85	theme	site	1721:1724	arg1	Occupancy					1699:1707	Occupancy	1699:1707	Occupancy of the N162 site, critical for antibody binding,	1699:1756	Occupancy of the N162 site, critical for antibody binding, appeared independent of N169 occupancy based on analysis of the T171A mutant protein.					
35712026	2	86	theme	antibody	321:328	arg1	therapies					330:338	antibody therapies	321:338	antibody therapies	321:338	One proven strategy to improve the efficacy of antibody therapies is to increase receptor binding affinity, in particular binding to FcγRIIIa/CD16a.					
35712026	9	87	from	CD16a	1511:1515	arg1	contribution					1493:1504	a smaller contribution	1483:1504	a smaller contribution from CD16a with four N-glycans	1483:1535	These observations were confirmed using intact protein mass spectrometry that demonstrated the predominant species corresponded to CD16a V158 with five N-glycans, with a smaller contribution from CD16a with four N-glycans.					
35712026	6	88	theme	N-glycosylation	922:936	arg1	level					913:917	The level	909:917	The level of N-glycosylation at each site	909:949	The level of N-glycosylation at each site is not known though computational prediction indicates low to moderate potential at each site based on primary sequence (40-70%).					
35712026	9	89	with	CD16a	1511:1515	arg1	N-glycans					1527:1535	four N-glycans	1522:1535	four N-glycans	1522:1535	These observations were confirmed using intact protein mass spectrometry that demonstrated the predominant species corresponded to CD16a V158 with five N-glycans, with a smaller contribution from CD16a with four N-glycans.					
35712026	3	90	theme	immune	561:566	arg1	responses					568:576	CD16a-mediated antibody-dependent immune responses	527:576	CD16a-mediated antibody-dependent immune responses	527:576	Currently, affinities are measured using recombinantly-expressed soluble extracellular FcγR domains and CD16a-mediated antibody-dependent immune responses are characterized using cultured cells.					
35712026	1	91	theme	monoclonal	211:220	arg1	antibodies					222:231	many therapeutic monoclonal antibodies	194:231	many therapeutic monoclonal antibodies	194:231	The antibody-binding Fc γ receptors (FcγRs) trigger life-saving immune responses and many therapeutic monoclonal antibodies require FcγR engagement for full effect.					
35712026	8	92	theme	CD16a	1228:1232	arg1	allotype					1239:1246	the tighter-binding CD16a V158 allotype	1208:1246	the tighter-binding CD16a V158 allotype	1208:1246	All five sites of the tighter-binding CD16a V158 allotype showed 65-100% N-glycan occupancy in proteomics-based experiments.					
35712026	11	93	theme	N169	1782:1785	arg1	occupancy					1787:1795	N169 occupancy	1782:1795	N169 occupancy based on analysis of the T171A mutant protein	1782:1841	Occupancy of the N162 site, critical for antibody binding, appeared independent of N169 occupancy based on analysis of the T171A mutant protein.					
35712026	11	94	theme	antibody	1740:1747	arg1	binding					1749:1755	antibody binding	1740:1755	antibody binding	1740:1755	Occupancy of the N162 site, critical for antibody binding, appeared independent of N169 occupancy based on analysis of the T171A mutant protein.					
35712026	0	95	theme	antibody-binding	4:19	arg1	IIIa					39:42	The antibody-binding Fc gamma receptor IIIa	0:42	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.	0:107	The antibody-binding Fc gamma receptor IIIa / CD16a is N-glycosylated with high occupancy at all five sites.					
37224006	2	0	theme	CMCSMA	517:522	arg1	hydrogel					554:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel	476:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF	476:601	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	1	1	theme	poor	265:268	arg1	concentration					307:319	uncontrollable concentration	292:319	uncontrollable concentration of growth factors	292:337	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	1	theme	poor	265:268	arg1	capabilities					278:289	poor storage capabilities	265:289	poor storage capabilities	265:289	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	1	theme	poor	265:268	arg1	etc					356:358	etc	356:358	etc	356:358	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	1	theme	poor	265:268	arg1	shape					349:353	unstable shape	340:353	unstable shape	340:353	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	0	2	theme	Defect	105:110	arg1	Repair					112:117	Efficient Bone Defect Repair	90:117	Efficient Bone Defect Repair	90:117	Lyophilized Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair.					
37224006	4	3	theme	LPRFe-loaded	720:731	arg1	hydrogel					733:740	The LPRFe-loaded hydrogel	716:740	The LPRFe-loaded hydrogel	716:740	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	1	4	theme	storage	270:276	arg1	concentration					307:319	uncontrollable concentration	292:319	uncontrollable concentration of growth factors	292:337	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	4	theme	storage	270:276	arg1	capabilities					278:289	poor storage capabilities	265:289	poor storage capabilities	265:289	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	4	theme	storage	270:276	arg1	etc					356:358	etc	356:358	etc	356:358	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	4	theme	storage	270:276	arg1	shape					349:353	unstable shape	340:353	unstable shape	340:353	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	3	5	contain	possessed	617:625	arg1	hydrogel					608:615	The hydrogel	604:615	The hydrogel	604:615	The hydrogel possessed suitable physical properties and sustainable release ability of growth factors in LPRFe.					
37224006	3	5	contain	possessed	617:625	arg2	properties					645:654	suitable physical properties	627:654	suitable physical properties	627:654	The hydrogel possessed suitable physical properties and sustainable release ability of growth factors in LPRFe.					
37224006	3	5	contain	possessed	617:625	arg2	ability					680:686	sustainable release ability	660:686	sustainable release ability	660:686	The hydrogel possessed suitable physical properties and sustainable release ability of growth factors in LPRFe.					
37224006	2	6	theme	GelMA	547:551	arg1	hydrogel					554:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel	476:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF	476:601	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	5	7	theme	LPRFe	1021:1025	arg1	introduction					1005:1016	the introduction	1001:1016	the introduction of LPRFe in the hydrogel	1001:1041	Furthermore, the animal experiments demonstrated that the hydrogel possessed excellent biocompatibility and biodegradability, and the introduction of LPRFe in the hydrogel can effectively accelerate the bone healing process.					
37224006	3	8	from	ability	680:686	arg1	LPRFe					709:713	LPRFe	709:713	LPRFe	709:713	The hydrogel possessed suitable physical properties and sustainable release ability of growth factors in LPRFe.					
37224006	2	9	theme	composite	403:411	arg1	hydrogel					413:420	a photocrosslinkable composite hydrogel	382:420	a photocrosslinkable composite hydrogel	382:420	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	2	10	theme	methacryloyl	533:544	arg1	hydrogel					554:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel	476:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF	476:601	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	2	11	theme	methacryloyl	503:514	arg1	hydrogel					554:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel	476:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF	476:601	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	4	12	theme	osteogenic	800:809	arg1	differentiation					811:825	osteogenic differentiation	800:825	osteogenic differentiation	800:825	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	3	13	from	properties	645:654	arg1	LPRFe					709:713	LPRFe	709:713	LPRFe	709:713	The hydrogel possessed suitable physical properties and sustainable release ability of growth factors in LPRFe.					
37224006	5	14	theme	animal	888:893	arg1	experiments					895:905	the animal experiments	884:905	the animal experiments	884:905	Furthermore, the animal experiments demonstrated that the hydrogel possessed excellent biocompatibility and biodegradability, and the introduction of LPRFe in the hydrogel can effectively accelerate the bone healing process.					
37224006	0	15	theme	Platelet-Rich	12:24	arg1	Hydrogel					77:84	Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel	12:84	Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair	12:117	Lyophilized Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair.					
37224006	2	16	theme	/gelatin	524:531	arg1	hydrogel					554:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel	476:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF	476:601	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	1	17	dep	factor	171:176	arg1	carrier					178:184	carrier	178:184	an autologous growth factor carrier that promotes bone tissue regeneration	150:223	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	18	theme	bone	200:203	arg1	regeneration					212:223	bone tissue regeneration	200:223	bone tissue regeneration	200:223	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	19	theme	uncontrollable	292:305	arg1	capabilities					278:289	poor storage capabilities	265:289	poor storage capabilities	265:289	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	19	theme	uncontrollable	292:305	arg1	concentration					307:319	uncontrollable concentration	292:319	uncontrollable concentration of growth factors	292:337	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	20	theme	Platelet-rich	120:132	arg1	factor					171:176	an autologous growth factor	150:176	an autologous growth factor carrier that promotes bone tissue regeneration	150:223	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	20	theme	Platelet-rich	120:132	arg1	PRF					142:144	PRF	142:144	PRF	142:144	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	20	theme	Platelet-rich	120:132	arg1	fibrin					134:139	Platelet-rich fibrin	120:139	Platelet-rich fibrin (PRF)	120:145	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	21	theme	tissue	205:210	arg1	regeneration					212:223	bone tissue regeneration	200:223	bone tissue regeneration	200:223	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	0	22	theme	Exudate-Loaded	33:46	arg1	Hydrogel					77:84	Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel	12:84	Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair	12:117	Lyophilized Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair.					
37224006	4	23	theme	mesenchymal	839:849	arg1	cells					856:860	rat bone mesenchymal stem cells	830:860	rat bone mesenchymal stem cells (BMSCs)	830:868	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	4	23	theme	mesenchymal	839:849	arg1	BMSCs					863:867	BMSCs	863:867	BMSCs	863:867	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	6	24	theme	bone	1206:1209	arg1	defects					1211:1217	bone defects	1206:1217	bone defects	1206:1217	Conclusively, the combination of LPRFe with CMCSMA/GelMA hydrogel may be a promising therapeutic approach for bone defects.					
37224006	0	25	theme	Fibrin	26:31	arg1	Hydrogel					77:84	Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel	12:84	Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair	12:117	Lyophilized Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair.					
37224006	6	26	theme	CMCSMA/GelMA	1140:1151	arg1	hydrogel					1153:1160	CMCSMA/GelMA hydrogel	1140:1160	CMCSMA/GelMA hydrogel	1140:1160	Conclusively, the combination of LPRFe with CMCSMA/GelMA hydrogel may be a promising therapeutic approach for bone defects.					
37224006	2	27	theme	PRF	451:453	arg1	LPRFe					464:468	LPRFe	464:468	LPRFe	464:468	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	2	27	theme	PRF	451:453	arg1	exudate					455:461	lyophilized PRF exudate	439:461	lyophilized PRF exudate (LPRFe)	439:469	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	0	28	theme	Chitosan/GelMA	62:75	arg1	Hydrogel					77:84	Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel	12:84	Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair	12:117	Lyophilized Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair.					
37224006	1	29	theme	growth	324:329	arg1	factors					331:337	growth factors	324:337	growth factors	324:337	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	6	30	with	combination	1114:1124	arg1	hydrogel					1153:1160	CMCSMA/GelMA hydrogel	1140:1160	CMCSMA/GelMA hydrogel	1140:1160	Conclusively, the combination of LPRFe with CMCSMA/GelMA hydrogel may be a promising therapeutic approach for bone defects.					
37224006	4	31	theme	cells	856:860	arg1	differentiation					811:825	osteogenic differentiation	800:825	osteogenic differentiation	800:825	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	4	31	theme	cells	856:860	arg1	proliferation					770:782	proliferation	770:782	proliferation	770:782	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	4	31	theme	cells	856:860	arg1	migration					785:793	migration	785:793	migration	785:793	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	4	31	theme	cells	856:860	arg1	adhesion					760:767	adhesion	760:767	adhesion	760:767	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	2	32	theme	photocrosslinkable	384:401	arg1	hydrogel					413:420	a photocrosslinkable composite hydrogel	382:420	a photocrosslinkable composite hydrogel	382:420	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	2	33	theme	lyophilized	439:449	arg1	LPRFe					464:468	LPRFe	464:468	LPRFe	464:468	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	2	33	theme	lyophilized	439:449	arg1	exudate					455:461	lyophilized PRF exudate	439:461	lyophilized PRF exudate (LPRFe)	439:469	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	2	34	theme	chitosan	494:501	arg1	hydrogel					554:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel	476:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF	476:601	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	1	35	theme	factors	331:337	arg1	capabilities					278:289	poor storage capabilities	265:289	poor storage capabilities	265:289	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	35	theme	factors	331:337	arg1	concentration					307:319	uncontrollable concentration	292:319	uncontrollable concentration of growth factors	292:337	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	0	36	theme	Carboxymethyl	48:60	arg1	Hydrogel					77:84	Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel	12:84	Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair	12:117	Lyophilized Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair.					
37224006	3	37	theme	sustainable	660:670	arg1	ability					680:686	sustainable release ability	660:686	sustainable release ability	660:686	The hydrogel possessed suitable physical properties and sustainable release ability of growth factors in LPRFe.					
37224006	4	38	theme	stem	851:854	arg1	cells					856:860	rat bone mesenchymal stem cells	830:860	rat bone mesenchymal stem cells (BMSCs)	830:868	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	4	38	theme	stem	851:854	arg1	BMSCs					863:867	BMSCs	863:867	BMSCs	863:867	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	2	39	theme	carboxymethyl	480:492	arg1	hydrogel					554:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel	476:561	the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF	476:601	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	2	40	theme	PRF	599:601	arg1	dilemma					588:594	the dilemma	584:594	the dilemma of PRF	584:601	Herein, we developed a photocrosslinkable composite hydrogel by incorporating lyophilized PRF exudate (LPRFe) into the carboxymethyl chitosan methacryloyl (CMCSMA)/gelatin methacryloyl (GelMA) hydrogel to effectively solve the dilemma of PRF.					
37224006	3	41	theme	release	672:678	arg1	ability					680:686	sustainable release ability	660:686	sustainable release ability	660:686	The hydrogel possessed suitable physical properties and sustainable release ability of growth factors in LPRFe.					
37224006	5	42	theme	healing	1079:1085	arg1	process					1087:1093	the bone healing process	1070:1093	the bone healing process	1070:1093	Furthermore, the animal experiments demonstrated that the hydrogel possessed excellent biocompatibility and biodegradability, and the introduction of LPRFe in the hydrogel can effectively accelerate the bone healing process.					
37224006	5	43	theme	excellent	948:956	arg1	biocompatibility					958:973	excellent biocompatibility	948:973	excellent biocompatibility	948:973	Furthermore, the animal experiments demonstrated that the hydrogel possessed excellent biocompatibility and biodegradability, and the introduction of LPRFe in the hydrogel can effectively accelerate the bone healing process.					
37224006	1	44	theme	unstable	340:347	arg1	capabilities					278:289	poor storage capabilities	265:289	poor storage capabilities	265:289	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	44	theme	unstable	340:347	arg1	shape					349:353	unstable shape	340:353	unstable shape	340:353	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	6	45	theme	therapeutic	1181:1191	arg1	combination					1114:1124	the combination	1110:1124	the combination of LPRFe with CMCSMA/GelMA hydrogel	1110:1160	Conclusively, the combination of LPRFe with CMCSMA/GelMA hydrogel may be a promising therapeutic approach for bone defects.					
37224006	6	45	theme	therapeutic	1181:1191	arg1	approach					1193:1200	a promising therapeutic approach	1169:1200	a promising therapeutic approach for bone defects	1169:1217	Conclusively, the combination of LPRFe with CMCSMA/GelMA hydrogel may be a promising therapeutic approach for bone defects.					
37224006	4	46	theme	bone	834:837	arg1	cells					856:860	rat bone mesenchymal stem cells	830:860	rat bone mesenchymal stem cells (BMSCs)	830:868	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	4	46	theme	bone	834:837	arg1	BMSCs					863:867	BMSCs	863:867	BMSCs	863:867	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	5	47	theme	bone	1074:1077	arg1	healing					1079:1085	the bone healing	1070:1085	the bone healing process	1070:1093	Furthermore, the animal experiments demonstrated that the hydrogel possessed excellent biocompatibility and biodegradability, and the introduction of LPRFe in the hydrogel can effectively accelerate the bone healing process.					
37224006	4	48	theme	rat	830:832	arg1	cells					856:860	rat bone mesenchymal stem cells	830:860	rat bone mesenchymal stem cells (BMSCs)	830:868	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	4	48	theme	rat	830:832	arg1	BMSCs					863:867	BMSCs	863:867	BMSCs	863:867	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	4	49	dep	adhesion	760:767	arg1	the					756:758	the	756:758	the	756:758	The LPRFe-loaded hydrogel could improve the adhesion, proliferation, migration, and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs).					
37224006	6	50	theme	promising	1171:1179	arg1	combination					1114:1124	the combination	1110:1124	the combination of LPRFe with CMCSMA/GelMA hydrogel	1110:1160	Conclusively, the combination of LPRFe with CMCSMA/GelMA hydrogel may be a promising therapeutic approach for bone defects.					
37224006	6	50	theme	promising	1171:1179	arg1	approach					1193:1200	a promising therapeutic approach	1169:1200	a promising therapeutic approach for bone defects	1169:1217	Conclusively, the combination of LPRFe with CMCSMA/GelMA hydrogel may be a promising therapeutic approach for bone defects.					
37224006	5	51	from	introduction	1005:1016	arg1	hydrogel					1034:1041	the hydrogel	1030:1041	the hydrogel	1030:1041	Furthermore, the animal experiments demonstrated that the hydrogel possessed excellent biocompatibility and biodegradability, and the introduction of LPRFe in the hydrogel can effectively accelerate the bone healing process.					
37224006	3	52	theme	growth	691:696	arg1	factors					698:704	growth factors	691:704	growth factors	691:704	The hydrogel possessed suitable physical properties and sustainable release ability of growth factors in LPRFe.					
37224006	5	53	contain	possessed	938:946	arg1	hydrogel					929:936	the hydrogel	925:936	the hydrogel	925:936	Furthermore, the animal experiments demonstrated that the hydrogel possessed excellent biocompatibility and biodegradability, and the introduction of LPRFe in the hydrogel can effectively accelerate the bone healing process.					
37224006	5	53	contain	possessed	938:946	arg2	biodegradability					979:994	biodegradability	979:994	biodegradability	979:994	Furthermore, the animal experiments demonstrated that the hydrogel possessed excellent biocompatibility and biodegradability, and the introduction of LPRFe in the hydrogel can effectively accelerate the bone healing process.					
37224006	5	53	contain	possessed	938:946	arg2	biocompatibility					958:973	excellent biocompatibility	948:973	excellent biocompatibility	948:973	Furthermore, the animal experiments demonstrated that the hydrogel possessed excellent biocompatibility and biodegradability, and the introduction of LPRFe in the hydrogel can effectively accelerate the bone healing process.					
37224006	0	54	theme	Bone	100:103	arg1	Repair					112:117	Efficient Bone Defect Repair	90:117	Efficient Bone Defect Repair	90:117	Lyophilized Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair.					
37224006	3	55	theme	factors	698:704	arg1	ability					680:686	sustainable release ability	660:686	sustainable release ability	660:686	The hydrogel possessed suitable physical properties and sustainable release ability of growth factors in LPRFe.					
37224006	3	55	theme	factors	698:704	arg1	properties					645:654	suitable physical properties	627:654	suitable physical properties	627:654	The hydrogel possessed suitable physical properties and sustainable release ability of growth factors in LPRFe.					
37224006	3	56	theme	suitable	627:634	arg1	properties					645:654	suitable physical properties	627:654	suitable physical properties	627:654	The hydrogel possessed suitable physical properties and sustainable release ability of growth factors in LPRFe.					
37224006	1	57	theme	autologous	153:162	arg1	fibrin					134:139	Platelet-rich fibrin	120:139	Platelet-rich fibrin (PRF)	120:145	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	57	theme	autologous	153:162	arg1	factor					171:176	an autologous growth factor	150:176	an autologous growth factor carrier that promotes bone tissue regeneration	150:223	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	6	58	theme	LPRFe	1129:1133	arg1	combination					1114:1124	the combination	1110:1124	the combination of LPRFe with CMCSMA/GelMA hydrogel	1110:1160	Conclusively, the combination of LPRFe with CMCSMA/GelMA hydrogel may be a promising therapeutic approach for bone defects.					
37224006	6	58	theme	LPRFe	1129:1133	arg1	approach					1193:1200	a promising therapeutic approach	1169:1200	a promising therapeutic approach for bone defects	1169:1217	Conclusively, the combination of LPRFe with CMCSMA/GelMA hydrogel may be a promising therapeutic approach for bone defects.					
37224006	0	59	theme	Efficient	90:98	arg1	Repair					112:117	Efficient Bone Defect Repair	90:117	Efficient Bone Defect Repair	90:117	Lyophilized Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair.					
37224006	3	60	theme	physical	636:643	arg1	properties					645:654	suitable physical properties	627:654	suitable physical properties	627:654	The hydrogel possessed suitable physical properties and sustainable release ability of growth factors in LPRFe.					
37224006	1	61	theme	growth	164:169	arg1	fibrin					134:139	Platelet-rich fibrin	120:139	Platelet-rich fibrin (PRF)	120:145	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
37224006	1	61	theme	growth	164:169	arg1	factor					171:176	an autologous growth factor	150:176	an autologous growth factor carrier that promotes bone tissue regeneration	150:223	Platelet-rich fibrin (PRF) is an autologous growth factor carrier that promotes bone tissue regeneration, but its effectiveness is restrained by poor storage capabilities, uncontrollable concentration of growth factors, unstable shape, etc.					
35950549	5	0	with	hydrogel	1005:1012	arg1	agent					1047:1051	a traditional cross-linking agent	1019:1051	a traditional cross-linking agent	1019:1051	Structures printed by using CNCMA-pHEMA hydrogels show auxetic behavior with greatly enhanced toughness and stretchability compared to the hydrogel with a traditional cross-linking agent.					
35950549	2	1	from	works	400:404	arg1	hydrogels					409:417	hydrogels	409:417	hydrogels due to their intrinsic brittleness	409:452	Although negative Poisson's ratio has been obtained in various base materials such as metals and polymers, there are very limited works on hydrogels due to their intrinsic brittleness.					
35950549	1	2	theme	high	154:157	arg1	applications					169:180	high potential applications	154:180	high potential applications	154:180	Materials with negative Poisson's ratio have attracted considerable attention and offered high potential applications as biomedical devices due to their ability to expand in every direction when stretched.					
35950549	1	2	theme	high	154:157	arg1	devices					196:202	biomedical devices	185:202	biomedical devices due to their ability to expand in every direction when stretched	185:267	Materials with negative Poisson's ratio have attracted considerable attention and offered high potential applications as biomedical devices due to their ability to expand in every direction when stretched.					
35950549	5	3	theme	CNCMA-pHEMA	894:904	arg1	hydrogels					906:914	CNCMA-pHEMA hydrogels	894:914	CNCMA-pHEMA hydrogels	894:914	Structures printed by using CNCMA-pHEMA hydrogels show auxetic behavior with greatly enhanced toughness and stretchability compared to the hydrogel with a traditional cross-linking agent.					
35950549	1	4	theme	potential	159:167	arg1	applications					169:180	high potential applications	154:180	high potential applications	154:180	Materials with negative Poisson's ratio have attracted considerable attention and offered high potential applications as biomedical devices due to their ability to expand in every direction when stretched.					
35950549	1	4	theme	potential	159:167	arg1	devices					196:202	biomedical devices	185:202	biomedical devices due to their ability to expand in every direction when stretched	185:267	Materials with negative Poisson's ratio have attracted considerable attention and offered high potential applications as biomedical devices due to their ability to expand in every direction when stretched.					
35950549	6	5	theme	advanced	1131:1138	arg1	stents					1219:1224	biodegradable oesophageal self-expandable stents	1177:1224	biodegradable oesophageal self-expandable stents	1177:1224	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	6	5	theme	advanced	1131:1138	arg1	devices					1161:1167	advanced flexible implantable devices	1131:1167	advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents	1131:1224	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	6	6	theme	oesophageal	1191:1201	arg1	stents					1219:1224	biodegradable oesophageal self-expandable stents	1177:1224	biodegradable oesophageal self-expandable stents	1177:1224	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	3	7	theme	macro-cross-linking	535:553	arg1	agent					555:559	a macro-cross-linking agent	533:559	a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures	533:652	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
35950549	4	8	theme	CNCMA-pHEMA	669:679	arg1	hydrogels					681:689	Our developed CNCMA-pHEMA hydrogels	655:689	Our developed CNCMA-pHEMA hydrogels	655:689	Our developed CNCMA-pHEMA hydrogels exhibit significant improvements in mechanical properties, which is attributed to the coexistence of multiple chemical and physical interactions between the pHEMA and CNCMAs.					
35950549	2	9	theme	due	419:421	arg1	hydrogels					409:417	hydrogels	409:417	hydrogels due to their intrinsic brittleness	409:452	Although negative Poisson's ratio has been obtained in various base materials such as metals and polymers, there are very limited works on hydrogels due to their intrinsic brittleness.					
35950549	5	10	with	behavior	929:936	arg1	toughness					960:968	greatly enhanced toughness	943:968	greatly enhanced toughness	943:968	Structures printed by using CNCMA-pHEMA hydrogels show auxetic behavior with greatly enhanced toughness and stretchability compared to the hydrogel with a traditional cross-linking agent.					
35950549	5	10	with	behavior	929:936	arg1	stretchability					974:987	stretchability	974:987	stretchability	974:987	Structures printed by using CNCMA-pHEMA hydrogels show auxetic behavior with greatly enhanced toughness and stretchability compared to the hydrogel with a traditional cross-linking agent.					
35950549	4	11	theme	mechanical	727:736	arg1	properties					738:747	mechanical properties	727:747	mechanical properties	727:747	Our developed CNCMA-pHEMA hydrogels exhibit significant improvements in mechanical properties, which is attributed to the coexistence of multiple chemical and physical interactions between the pHEMA and CNCMAs.					
35950549	2	12	theme	negative	279:286	arg1	ratio					298:302	negative Poisson's ratio	279:302	negative Poisson's ratio	279:302	Although negative Poisson's ratio has been obtained in various base materials such as metals and polymers, there are very limited works on hydrogels due to their intrinsic brittleness.					
35950549	6	13	theme	tough	1070:1074	arg1	hydrogels					1084:1092	Such strong and tough auxetic hydrogels	1054:1092	Such strong and tough auxetic hydrogels	1054:1092	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	0	14	theme	Soft	39:42	arg1	Structures					52:61	Soft Auxetic Structures	39:61	Soft Auxetic Structures	39:61	Toughened Hydrogels for 3D Printing of Soft Auxetic Structures.					
35950549	4	15	dep	pHEMA	848:852	arg1	the					844:846	the	844:846	the	844:846	Our developed CNCMA-pHEMA hydrogels exhibit significant improvements in mechanical properties, which is attributed to the coexistence of multiple chemical and physical interactions between the pHEMA and CNCMAs.					
35950549	1	16	theme	negative	79:86	arg1	ratio					98:102	negative Poisson's ratio	79:102	negative Poisson's ratio	79:102	Materials with negative Poisson's ratio have attracted considerable attention and offered high potential applications as biomedical devices due to their ability to expand in every direction when stretched.					
35950549	1	17	theme	biomedical	185:194	arg1	applications					169:180	high potential applications	154:180	high potential applications	154:180	Materials with negative Poisson's ratio have attracted considerable attention and offered high potential applications as biomedical devices due to their ability to expand in every direction when stretched.					
35950549	1	17	theme	biomedical	185:194	arg1	devices					196:202	biomedical devices	185:202	biomedical devices due to their ability to expand in every direction when stretched	185:267	Materials with negative Poisson's ratio have attracted considerable attention and offered high potential applications as biomedical devices due to their ability to expand in every direction when stretched.					
35950549	0	18	theme	Toughened	0:8	arg1	Hydrogels					10:18	Toughened Hydrogels	0:18	Toughened Hydrogels for 3D Printing of Soft Auxetic Structures.	0:62	Toughened Hydrogels for 3D Printing of Soft Auxetic Structures.					
35950549	5	19	theme	enhanced	951:958	arg1	toughness					960:968	greatly enhanced toughness	943:968	greatly enhanced toughness	943:968	Structures printed by using CNCMA-pHEMA hydrogels show auxetic behavior with greatly enhanced toughness and stretchability compared to the hydrogel with a traditional cross-linking agent.					
35950549	0	20	theme	3D	24:25	arg1	Printing					27:34	3D Printing	24:34	3D Printing of Soft Auxetic Structures	24:61	Toughened Hydrogels for 3D Printing of Soft Auxetic Structures.					
35950549	4	21	theme	physical	814:821	arg1	interactions					823:834	multiple chemical and physical interactions	792:834	multiple chemical and physical interactions between the pHEMA and CNCMAs	792:863	Our developed CNCMA-pHEMA hydrogels exhibit significant improvements in mechanical properties, which is attributed to the coexistence of multiple chemical and physical interactions between the pHEMA and CNCMAs.					
35950549	6	22	theme	self-expandable	1203:1217	arg1	stents					1219:1224	biodegradable oesophageal self-expandable stents	1177:1224	biodegradable oesophageal self-expandable stents	1177:1224	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	1	23	theme	due	204:206	arg1	applications					169:180	high potential applications	154:180	high potential applications	154:180	Materials with negative Poisson's ratio have attracted considerable attention and offered high potential applications as biomedical devices due to their ability to expand in every direction when stretched.					
35950549	1	23	theme	due	204:206	arg1	devices					196:202	biomedical devices	185:202	biomedical devices due to their ability to expand in every direction when stretched	185:267	Materials with negative Poisson's ratio have attracted considerable attention and offered high potential applications as biomedical devices due to their ability to expand in every direction when stretched.					
35950549	3	24	theme	nanocrystals	508:519	arg1	use					477:479	the use	473:479	the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures	473:652	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
35950549	4	25	theme	developed	659:667	arg1	hydrogels					681:689	Our developed CNCMA-pHEMA hydrogels	655:689	Our developed CNCMA-pHEMA hydrogels	655:689	Our developed CNCMA-pHEMA hydrogels exhibit significant improvements in mechanical properties, which is attributed to the coexistence of multiple chemical and physical interactions between the pHEMA and CNCMAs.					
35950549	6	26	theme	implantable	1149:1159	arg1	stents					1219:1224	biodegradable oesophageal self-expandable stents	1177:1224	biodegradable oesophageal self-expandable stents	1177:1224	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	6	26	theme	implantable	1149:1159	arg1	devices					1161:1167	advanced flexible implantable devices	1131:1167	advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents	1131:1224	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	5	27	theme	auxetic	921:927	arg1	behavior					929:936	auxetic behavior	921:936	auxetic behavior with greatly enhanced toughness and stretchability compared to the hydrogel with a traditional cross-linking agent	921:1051	Structures printed by using CNCMA-pHEMA hydrogels show auxetic behavior with greatly enhanced toughness and stretchability compared to the hydrogel with a traditional cross-linking agent.					
35950549	3	28	theme	3D	620:621	arg1	printing					623:630	3D printing	620:630	3D printing of auxetic structures	620:652	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
35950549	5	29	theme	cross-linking	1033:1045	arg1	agent					1047:1051	a traditional cross-linking agent	1019:1051	a traditional cross-linking agent	1019:1051	Structures printed by using CNCMA-pHEMA hydrogels show auxetic behavior with greatly enhanced toughness and stretchability compared to the hydrogel with a traditional cross-linking agent.					
35950549	2	30	theme	limited	392:398	arg1	works					400:404	very limited works	387:404	very limited works on hydrogels due to their intrinsic brittleness	387:452	Although negative Poisson's ratio has been obtained in various base materials such as metals and polymers, there are very limited works on hydrogels due to their intrinsic brittleness.					
35950549	3	31	theme	2-hydroxyethyl	569:582	arg1	poly					564:567	poly	564:567	poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels	564:614	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
35950549	3	31	theme	2-hydroxyethyl	569:582	arg1	methacrylate					584:595	2-hydroxyethyl methacrylate	569:595	2-hydroxyethyl methacrylate	569:595	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
35950549	6	32	theme	strong	1059:1064	arg1	hydrogels					1084:1092	Such strong and tough auxetic hydrogels	1054:1092	Such strong and tough auxetic hydrogels	1054:1092	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	0	33	theme	Auxetic	44:50	arg1	Structures					52:61	Soft Auxetic Structures	39:61	Soft Auxetic Structures	39:61	Toughened Hydrogels for 3D Printing of Soft Auxetic Structures.					
35950549	4	34	theme	interactions	823:834	arg1	coexistence					777:787	the coexistence	773:787	the coexistence of multiple chemical and physical interactions between the pHEMA and CNCMAs	773:863	Our developed CNCMA-pHEMA hydrogels exhibit significant improvements in mechanical properties, which is attributed to the coexistence of multiple chemical and physical interactions between the pHEMA and CNCMAs.					
35950549	6	35	theme	biodegradable	1177:1189	arg1	stents					1219:1224	biodegradable oesophageal self-expandable stents	1177:1224	biodegradable oesophageal self-expandable stents	1177:1224	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	2	36	theme	intrinsic	432:440	arg1	brittleness					442:452	their intrinsic brittleness	426:452	their intrinsic brittleness	426:452	Although negative Poisson's ratio has been obtained in various base materials such as metals and polymers, there are very limited works on hydrogels due to their intrinsic brittleness.					
35950549	3	37	theme	auxetic	635:641	arg1	structures					643:652	auxetic structures	635:652	auxetic structures	635:652	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
35950549	3	38	theme	poly	564:567	arg1	hydrogels					606:614	poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels	564:614	poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels	564:614	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
35950549	2	39	theme	base	333:336	arg1	polymers					367:374	polymers	367:374	polymers	367:374	Although negative Poisson's ratio has been obtained in various base materials such as metals and polymers, there are very limited works on hydrogels due to their intrinsic brittleness.					
35950549	2	39	theme	base	333:336	arg1	materials					338:346	various base materials	325:346	various base materials such as metals and polymers	325:374	Although negative Poisson's ratio has been obtained in various base materials such as metals and polymers, there are very limited works on hydrogels due to their intrinsic brittleness.					
35950549	2	39	theme	base	333:336	arg1	metals					356:361	metals	356:361	metals	356:361	Although negative Poisson's ratio has been obtained in various base materials such as metals and polymers, there are very limited works on hydrogels due to their intrinsic brittleness.					
35950549	4	40	from	improvements	711:722	arg1	properties					738:747	mechanical properties	727:747	mechanical properties	727:747	Our developed CNCMA-pHEMA hydrogels exhibit significant improvements in mechanical properties, which is attributed to the coexistence of multiple chemical and physical interactions between the pHEMA and CNCMAs.					
35950549	3	41	theme	structures	643:652	arg1	printing					623:630	3D printing	620:630	3D printing of auxetic structures	620:652	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
35950549	4	42	theme	chemical	801:808	arg1	interactions					823:834	multiple chemical and physical interactions	792:834	multiple chemical and physical interactions between the pHEMA and CNCMAs	792:863	Our developed CNCMA-pHEMA hydrogels exhibit significant improvements in mechanical properties, which is attributed to the coexistence of multiple chemical and physical interactions between the pHEMA and CNCMAs.					
35950549	1	43	theme	considerable	119:130	arg1	attention					132:140	considerable attention	119:140	considerable attention	119:140	Materials with negative Poisson's ratio have attracted considerable attention and offered high potential applications as biomedical devices due to their ability to expand in every direction when stretched.					
35950549	6	44	theme	Such	1054:1057	arg1	hydrogels					1084:1092	Such strong and tough auxetic hydrogels	1054:1092	Such strong and tough auxetic hydrogels	1054:1092	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	4	45	theme	significant	699:709	arg1	improvements					711:722	significant improvements	699:722	significant improvements	699:722	Our developed CNCMA-pHEMA hydrogels exhibit significant improvements in mechanical properties, which is attributed to the coexistence of multiple chemical and physical interactions between the pHEMA and CNCMAs.					
35950549	2	46	theme	various	325:331	arg1	polymers					367:374	polymers	367:374	polymers	367:374	Although negative Poisson's ratio has been obtained in various base materials such as metals and polymers, there are very limited works on hydrogels due to their intrinsic brittleness.					
35950549	2	46	theme	various	325:331	arg1	materials					338:346	various base materials	325:346	various base materials such as metals and polymers	325:374	Although negative Poisson's ratio has been obtained in various base materials such as metals and polymers, there are very limited works on hydrogels due to their intrinsic brittleness.					
35950549	2	46	theme	various	325:331	arg1	metals					356:361	metals	356:361	metals	356:361	Although negative Poisson's ratio has been obtained in various base materials such as metals and polymers, there are very limited works on hydrogels due to their intrinsic brittleness.					
35950549	6	47	theme	flexible	1140:1147	arg1	stents					1219:1224	biodegradable oesophageal self-expandable stents	1177:1224	biodegradable oesophageal self-expandable stents	1177:1224	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	6	47	theme	flexible	1140:1147	arg1	devices					1161:1167	advanced flexible implantable devices	1131:1167	advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents	1131:1224	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	4	48	theme	multiple	792:799	arg1	interactions					823:834	multiple chemical and physical interactions	792:834	multiple chemical and physical interactions between the pHEMA and CNCMAs	792:863	Our developed CNCMA-pHEMA hydrogels exhibit significant improvements in mechanical properties, which is attributed to the coexistence of multiple chemical and physical interactions between the pHEMA and CNCMAs.					
35950549	6	49	theme	auxetic	1076:1082	arg1	hydrogels					1084:1092	Such strong and tough auxetic hydrogels	1054:1092	Such strong and tough auxetic hydrogels	1054:1092	Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.					
35950549	3	50	theme	methacrylated	484:496	arg1	CNCMAs					522:527	CNCMAs	522:527	CNCMAs	522:527	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
35950549	3	50	theme	methacrylated	484:496	arg1	nanocrystals					508:519	methacrylated cellulose nanocrystals	484:519	methacrylated cellulose nanocrystals (CNCMAs)	484:528	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
35950549	3	51	from	agent	555:559	arg1	hydrogels					606:614	poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels	564:614	poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels	564:614	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
35950549	0	52	theme	Structures	52:61	arg1	Printing					27:34	3D Printing	24:34	3D Printing of Soft Auxetic Structures	24:61	Toughened Hydrogels for 3D Printing of Soft Auxetic Structures.					
35950549	5	53	theme	traditional	1021:1031	arg1	agent					1047:1051	a traditional cross-linking agent	1019:1051	a traditional cross-linking agent	1019:1051	Structures printed by using CNCMA-pHEMA hydrogels show auxetic behavior with greatly enhanced toughness and stretchability compared to the hydrogel with a traditional cross-linking agent.					
35950549	3	54	theme	cellulose	498:506	arg1	CNCMAs					522:527	CNCMAs	522:527	CNCMAs	522:527	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
35950549	3	54	theme	cellulose	498:506	arg1	nanocrystals					508:519	methacrylated cellulose nanocrystals	484:519	methacrylated cellulose nanocrystals (CNCMAs)	484:528	Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures.					
36109538	4	0	theme	cationic	868:875	arg1	contaminants					877:888	the cationic contaminants	864:888	the cationic contaminants	864:888	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	10	1	theme	wastewater	1885:1894	arg1	samples					1896:1902	wastewater samples	1885:1902	wastewater samples	1885:1902	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	3	2	theme	promising	543:551	arg1	composite					528:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	2	theme	promising	543:551	arg1	green					553:557	a promising green	541:557	a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions	541:681	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	3	theme	cationic	583:590	arg1	methylene					592:600	the cationic methylene blue	579:605	the cationic methylene blue (MB)	579:610	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	3	theme	cationic	583:590	arg1	MB					608:609	MB	608:609	MB	608:609	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	1	4	theme	human	284:288	arg1	survival					290:297	human survival	284:297	human survival	284:297	The jeopardy of the synthetic dyes effluents on human health and the environment has swiftly aggravated, threatening human survival.					
36109538	10	5	theme	neutral	1911:1917	arg1	medium					1922:1927	the neutral pH medium	1907:1927	the neutral pH medium	1907:1927	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	6	6	theme	flawless	1222:1229	arg1	separation					1231:1240	facile, fast, and flawless separation	1204:1240	facile, fast, and flawless separation	1204:1240	Furthermore, the ferromagnetic behavior of the MMT-mAmCs composite is additional merit to our adsorbent that provides facile, fast, and flawless separation.					
36109538	1	7	theme	synthetic	187:195	arg1	dyes					197:200	the synthetic dyes effluents on human health and the environment	183:246	dyes	197:200	The jeopardy of the synthetic dyes effluents on human health and the environment has swiftly aggravated, threatening human survival.					
36109538	9	8	theme	satisfactory	1651:1662	arg1	characteristics					1675:1689	satisfactory adsorption characteristics	1651:1689	satisfactory adsorption characteristics	1651:1689	Besides, the advanced adsorbent preserved satisfactory adsorption characteristics with maximal removal efficacy exceeding 87% after reuse for ten consecutive cycles.					
36109538	6	9	theme	MMT-mAmCs	1133:1141	arg1	composite					1143:1151	the MMT-mAmCs composite	1129:1151	the MMT-mAmCs composite	1129:1151	Furthermore, the ferromagnetic behavior of the MMT-mAmCs composite is additional merit to our adsorbent that provides facile, fast, and flawless separation.					
36109538	0	10	theme	@	125:125	arg1	composite					156:164	reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite	93:164	reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite	93:164	Efficient removal of noxious methylene blue and crystal violet dyes at neutral conditions by reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite.					
36109538	3	11	theme	blue	602:605	arg1	methylene					592:600	the cationic methylene blue	579:605	the cationic methylene blue (MB)	579:610	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	11	theme	blue	602:605	arg1	MB					608:609	MB	608:609	MB	608:609	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	1	12	theme	human	215:219	arg1	health					221:226	human health	215:226	human health	215:226	The jeopardy of the synthetic dyes effluents on human health and the environment has swiftly aggravated, threatening human survival.					
36109538	9	13	theme	maximal	1696:1702	arg1	efficacy					1712:1719	maximal removal efficacy	1696:1719	maximal removal efficacy exceeding 87% after reuse for ten consecutive cycles	1696:1772	Besides, the advanced adsorbent preserved satisfactory adsorption characteristics with maximal removal efficacy exceeding 87% after reuse for ten consecutive cycles.					
36109538	6	14	theme	fast	1212:1215	arg1	separation					1231:1240	facile, fast, and flawless separation	1204:1240	facile, fast, and flawless separation	1204:1240	Furthermore, the ferromagnetic behavior of the MMT-mAmCs composite is additional merit to our adsorbent that provides facile, fast, and flawless separation.					
36109538	0	15	theme	chitosan	147:154	arg1	composite					156:164	reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite	93:164	reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite	93:164	Efficient removal of noxious methylene blue and crystal violet dyes at neutral conditions by reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite.					
36109538	10	16	from	medium	1922:1927	arg1	samples					1896:1902	wastewater samples	1885:1902	wastewater samples	1885:1902	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	10	16	from	medium	1922:1927	arg1	water					1857:1861	real agricultural water	1839:1861	real agricultural water	1839:1861	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	10	16	from	medium	1922:1927	arg1	water					1875:1879	Nile river water	1864:1879	Nile river water	1864:1879	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	6	17	theme	facile	1204:1209	arg1	separation					1231:1240	facile, fast, and flawless separation	1204:1240	facile, fast, and flawless separation	1204:1240	Furthermore, the ferromagnetic behavior of the MMT-mAmCs composite is additional merit to our adsorbent that provides facile, fast, and flawless separation.					
36109538	4	18	theme	negative	732:739	arg1	charges					741:747	high negative charges	727:747	high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants	727:888	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	2	19	dep	figured	330:336	arg1	out					338:340	out	338:340	out	338:340	Hence, sustained studies have figured out the most acceptable way to eliminate this poisonous contaminant.					
36109538	10	20	theme	adsorptive	1996:2005	arg1	pollutants					2024:2033	adsorptive removal cationic pollutants	1996:2033	adsorptive removal cationic pollutants from their aquatic media	1996:2058	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	7	21	theme	adsorptive	1329:1338	arg1	removal					1340:1346	the adsorptive removal	1325:1346	the adsorptive removal of MB and CV	1325:1359	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	4	22	theme	MMT-mAmCs	699:707	arg1	composite					709:717	MMT-mAmCs composite	699:717	MMT-mAmCs composite	699:717	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	0	23	theme	violet	56:61	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of noxious methylene blue and crystal violet	0:61	Efficient removal of noxious methylene blue and crystal violet dyes at neutral conditions by reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite.					
36109538	4	24	from	11	778:779	arg1	range					762:766	a wide pH range	752:766	a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants	752:888	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	9	25	theme	consecutive	1755:1765	arg1	cycles					1767:1772	ten consecutive cycles	1751:1772	ten consecutive cycles	1751:1772	Besides, the advanced adsorbent preserved satisfactory adsorption characteristics with maximal removal efficacy exceeding 87% after reuse for ten consecutive cycles.					
36109538	8	26	theme	isotherm	1464:1471	arg1	investigatins					1485:1497	the isotherm and kinetic investigatins	1460:1497	investigatins	1485:1497	Moreover, the isotherm and kinetic investigatins depicted that the adsorption of both cationic dyes fitted Langmuir and Pseudo 2nd order models, respectively.					
36109538	10	27	theme	cationic	2015:2022	arg1	pollutants					2024:2033	adsorptive removal cationic pollutants	1996:2033	adsorptive removal cationic pollutants from their aquatic media	1996:2058	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	5	28	theme	simple	1059:1064	arg1	conditions					1074:1083	simple process conditions	1059:1083	simple process conditions	1059:1083	In addition, the experimental study confirmed that the optimum pH to adsorb both MB and CV was pH 7, inferring the ability of MMT-mAmCs to adsorb both cationic dyes in simple process conditions.					
36109538	7	29	theme	CV	1410:1411	arg1	capacity					1391:1398	the maximum adsorption capacity	1368:1398	the maximum adsorption capacity of MB and CV	1368:1411	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	7	29	theme	CV	1410:1411	arg1	137					1418:1420	137	1418:1420	137	1418:1420	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	8	30	theme	kinetic	1477:1483	arg1	investigatins					1485:1497	the isotherm and kinetic investigatins	1460:1497	investigatins	1485:1497	Moreover, the isotherm and kinetic investigatins depicted that the adsorption of both cationic dyes fitted Langmuir and Pseudo 2nd order models, respectively.					
36109538	3	31	theme	montmorillonite/magnetic	453:476	arg1	composite					528:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	31	theme	montmorillonite/magnetic	453:476	arg1	green					553:557	a promising green	541:557	a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions	541:681	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	0	32	theme	reusable	93:100	arg1	composite					156:164	reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite	93:164	reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite	93:164	Efficient removal of noxious methylene blue and crystal violet dyes at neutral conditions by reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite.					
36109538	4	33	from	range	762:766	arg1	charges					741:747	high negative charges	727:747	high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants	727:888	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	4	34	theme	wide	754:757	arg1	range					762:766	a wide pH range	752:766	a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants	752:888	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	0	35	theme	Efficient	0:8	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of noxious methylene blue and crystal violet	0:61	Efficient removal of noxious methylene blue and crystal violet dyes at neutral conditions by reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite.					
36109538	5	36	theme	optimum	946:952	arg1	pH					954:955	the optimum pH	942:955	the optimum pH to adsorb both MB and CV	942:980	In addition, the experimental study confirmed that the optimum pH to adsorb both MB and CV was pH 7, inferring the ability of MMT-mAmCs to adsorb both cationic dyes in simple process conditions.					
36109538	5	36	theme	optimum	946:952	arg1	pH					986:987	pH 7	986:989	pH 7	986:989	In addition, the experimental study confirmed that the optimum pH to adsorb both MB and CV was pH 7, inferring the ability of MMT-mAmCs to adsorb both cationic dyes in simple process conditions.					
36109538	3	37	theme	chitosan	507:514	arg1	composite					528:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	37	theme	chitosan	507:514	arg1	green					553:557	a promising green	541:557	a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions	541:681	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	38	dep	methylene	592:600	arg1	dyes					636:639	dyes	636:639	dyes	636:639	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	10	39	theme	agricultural	1844:1855	arg1	water					1857:1861	real agricultural water	1839:1861	real agricultural water	1839:1861	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	1	40	dep	dyes	197:200	arg1	effluents					202:210	effluents	202:210	effluents on human health	202:226	The jeopardy of the synthetic dyes effluents on human health and the environment has swiftly aggravated, threatening human survival.					
36109538	3	41	theme	@	485:485	arg1	composite					528:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	41	theme	@	485:485	arg1	green					553:557	a promising green	541:557	a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions	541:681	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	0	42	theme	methylene	29:37	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of noxious methylene blue and crystal violet	0:61	Efficient removal of noxious methylene blue and crystal violet dyes at neutral conditions by reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite.					
36109538	8	43	theme	cationic	1536:1543	arg1	dyes					1545:1548	both cationic dyes	1531:1548	both cationic dyes	1531:1548	Moreover, the isotherm and kinetic investigatins depicted that the adsorption of both cationic dyes fitted Langmuir and Pseudo 2nd order models, respectively.					
36109538	2	44	theme	acceptable	351:360	arg1	way					362:364	the most acceptable way	342:364	the most acceptable way to eliminate this poisonous contaminant	342:404	Hence, sustained studies have figured out the most acceptable way to eliminate this poisonous contaminant.					
36109538	10	45	theme	river	1869:1873	arg1	water					1875:1879	Nile river water	1864:1879	Nile river water	1864:1879	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	3	46	theme	MMT-mAmCs	517:525	arg1	composite					528:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	46	theme	MMT-mAmCs	517:525	arg1	green					553:557	a promising green	541:557	a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions	541:681	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	7	47	theme	MB	1403:1404	arg1	capacity					1391:1398	the maximum adsorption capacity	1368:1398	the maximum adsorption capacity of MB and CV	1368:1411	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	7	47	theme	MB	1403:1404	arg1	137					1418:1420	137	1418:1420	137	1418:1420	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	10	48	theme	aquatic	2046:2052	arg1	media					2054:2058	their aquatic media	2040:2058	their aquatic media	2040:2058	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	4	49	theme	zeta	799:802	arg1	measurements					814:825	zeta potential measurements	799:825	zeta potential measurements	799:825	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	8	50	theme	2nd	1577:1579	arg1	models					1587:1592	Pseudo 2nd order models	1570:1592	Pseudo 2nd order models	1570:1592	Moreover, the isotherm and kinetic investigatins depicted that the adsorption of both cationic dyes fitted Langmuir and Pseudo 2nd order models, respectively.					
36109538	4	51	contain	carries	719:725	arg1	composite					709:717	MMT-mAmCs composite	699:717	MMT-mAmCs composite	699:717	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	4	51	contain	carries	719:725	arg2	charges					741:747	high negative charges	727:747	high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants	727:888	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	7	52	theme	adsorption	1380:1389	arg1	capacity					1391:1398	the maximum adsorption capacity	1368:1398	the maximum adsorption capacity of MB and CV	1368:1411	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	7	52	theme	adsorption	1380:1389	arg1	137					1418:1420	137	1418:1420	137	1418:1420	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	6	53	theme	additional	1156:1165	arg1	behavior					1117:1124	the ferromagnetic behavior	1099:1124	the ferromagnetic behavior of the MMT-mAmCs composite	1099:1151	Furthermore, the ferromagnetic behavior of the MMT-mAmCs composite is additional merit to our adsorbent that provides facile, fast, and flawless separation.					
36109538	6	53	theme	additional	1156:1165	arg1	merit					1167:1171	additional merit	1156:1171	additional merit to our adsorbent that provides facile, fast, and flawless separation	1156:1240	Furthermore, the ferromagnetic behavior of the MMT-mAmCs composite is additional merit to our adsorbent that provides facile, fast, and flawless separation.					
36109538	3	54	from	wastewater	650:659	arg1	conditions					672:681	neutral conditions	664:681	neutral conditions	664:681	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	10	55	theme	pH	1919:1920	arg1	medium					1922:1927	the neutral pH medium	1907:1927	the neutral pH medium	1907:1927	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	6	56	theme	composite	1143:1151	arg1	behavior					1117:1124	the ferromagnetic behavior	1099:1124	the ferromagnetic behavior of the MMT-mAmCs composite	1099:1151	Furthermore, the ferromagnetic behavior of the MMT-mAmCs composite is additional merit to our adsorbent that provides facile, fast, and flawless separation.					
36109538	6	56	theme	composite	1143:1151	arg1	merit					1167:1171	additional merit	1156:1171	additional merit to our adsorbent that provides facile, fast, and flawless separation	1156:1240	Furthermore, the ferromagnetic behavior of the MMT-mAmCs composite is additional merit to our adsorbent that provides facile, fast, and flawless separation.					
36109538	1	57	theme	dyes	197:200	arg1	jeopardy					171:178	The jeopardy	167:178	The jeopardy of the synthetic dyes effluents on human health and the environment	167:246	The jeopardy of the synthetic dyes effluents on human health and the environment has swiftly aggravated, threatening human survival.					
36109538	9	58	theme	adsorption	1664:1673	arg1	characteristics					1675:1689	satisfactory adsorption characteristics	1651:1689	satisfactory adsorption characteristics	1651:1689	Besides, the advanced adsorbent preserved satisfactory adsorption characteristics with maximal removal efficacy exceeding 87% after reuse for ten consecutive cycles.					
36109538	0	59	theme	amine-functionalized	126:145	arg1	composite					156:164	reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite	93:164	reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite	93:164	Efficient removal of noxious methylene blue and crystal violet dyes at neutral conditions by reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite.					
36109538	7	60	theme	maximum	1372:1378	arg1	capacity					1391:1398	the maximum adsorption capacity	1368:1398	the maximum adsorption capacity of MB and CV	1368:1411	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	7	60	theme	maximum	1372:1378	arg1	137					1418:1420	137	1418:1420	137	1418:1420	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	5	61	theme	cationic	1042:1049	arg1	dyes					1051:1054	both cationic dyes	1037:1054	both cationic dyes	1037:1054	In addition, the experimental study confirmed that the optimum pH to adsorb both MB and CV was pH 7, inferring the ability of MMT-mAmCs to adsorb both cationic dyes in simple process conditions.					
36109538	3	62	theme	crystal	616:622	arg1	CV					632:633	CV	632:633	CV	632:633	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	62	theme	crystal	616:622	arg1	violet					624:629	crystal violet	616:629	crystal violet (CV)	616:634	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	9	63	theme	removal	1704:1710	arg1	efficacy					1712:1719	maximal removal efficacy	1696:1719	maximal removal efficacy exceeding 87% after reuse for ten consecutive cycles	1696:1772	Besides, the advanced adsorbent preserved satisfactory adsorption characteristics with maximal removal efficacy exceeding 87% after reuse for ten consecutive cycles.					
36109538	5	64	theme	experimental	908:919	arg1	study					921:925	the experimental study	904:925	the experimental study	904:925	In addition, the experimental study confirmed that the optimum pH to adsorb both MB and CV was pH 7, inferring the ability of MMT-mAmCs to adsorb both cationic dyes in simple process conditions.					
36109538	7	65	theme	CV	1358:1359	arg1	removal					1340:1346	the adsorptive removal	1325:1346	the adsorptive removal of MB and CV	1325:1359	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	4	66	theme	high	727:730	arg1	charges					741:747	high negative charges	727:747	high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants	727:888	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	5	67	theme	MMT-mAmCs	1017:1025	arg1	ability					1006:1012	the ability	1002:1012	the ability of MMT-mAmCs to adsorb both cationic dyes in simple process conditions	1002:1083	In addition, the experimental study confirmed that the optimum pH to adsorb both MB and CV was pH 7, inferring the ability of MMT-mAmCs to adsorb both cationic dyes in simple process conditions.					
36109538	7	68	theme	MB	1351:1352	arg1	removal					1340:1346	the adsorptive removal	1325:1346	the adsorptive removal of MB and CV	1325:1359	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	1	69	from	effluents	202:210	arg1	health					221:226	human health	215:226	human health	215:226	The jeopardy of the synthetic dyes effluents on human health and the environment has swiftly aggravated, threatening human survival.					
36109538	4	70	dep	11	778:779	arg1	to					775:776	to	775:776	to	775:776	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	10	71	theme	reusable	1973:1980	arg1	candidate					1982:1990	a superb reusable candidate	1964:1990	a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media	1964:2058	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	0	72	theme	neutral	71:77	arg1	conditions					79:88	neutral conditions	71:88	neutral conditions	71:88	Efficient removal of noxious methylene blue and crystal violet dyes at neutral conditions by reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite.					
36109538	10	73	theme	Nile	1864:1867	arg1	water					1875:1879	Nile river water	1864:1879	Nile river water	1864:1879	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	2	74	theme	sustained	307:315	arg1	studies					317:323	sustained studies	307:323	sustained studies	307:323	Hence, sustained studies have figured out the most acceptable way to eliminate this poisonous contaminant.					
36109538	10	75	theme	removal	2007:2013	arg1	pollutants					2024:2033	adsorptive removal cationic pollutants	1996:2033	adsorptive removal cationic pollutants from their aquatic media	1996:2058	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	0	76	theme	montmorillonite/NiFe2O4	102:124	arg1	composite					156:164	reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite	93:164	reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite	93:164	Efficient removal of noxious methylene blue and crystal violet dyes at neutral conditions by reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite.					
36109538	5	77	theme	process	1066:1072	arg1	conditions					1074:1083	simple process conditions	1059:1083	simple process conditions	1059:1083	In addition, the experimental study confirmed that the optimum pH to adsorb both MB and CV was pH 7, inferring the ability of MMT-mAmCs to adsorb both cationic dyes in simple process conditions.					
36109538	10	78	from	media	2054:2058	arg1	pollutants					2024:2033	adsorptive removal cationic pollutants	1996:2033	adsorptive removal cationic pollutants from their aquatic media	1996:2058	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	3	79	theme	NiFe2O4	478:484	arg1	composite					528:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	79	theme	NiFe2O4	478:484	arg1	green					553:557	a promising green	541:557	a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions	541:681	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	7	80	theme	auspicious	1292:1301	arg1	adsorbability					1303:1315	an auspicious adsorbability	1289:1315	an auspicious adsorbability towards the adsorptive removal of MB and CV	1289:1359	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	2	81	theme	poisonous	384:392	arg1	contaminant					394:404	this poisonous contaminant	379:404	this poisonous contaminant	379:404	Hence, sustained studies have figured out the most acceptable way to eliminate this poisonous contaminant.					
36109538	0	82	theme	noxious	21:27	arg1	methylene					29:37	noxious methylene blue	21:42	noxious methylene blue	21:42	Efficient removal of noxious methylene blue and crystal violet dyes at neutral conditions by reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite.					
36109538	4	83	theme	pH	759:760	arg1	range					762:766	a wide pH range	752:766	a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants	752:888	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	0	84	theme	blue	39:42	arg1	methylene					29:37	noxious methylene blue	21:42	noxious methylene blue	21:42	Efficient removal of noxious methylene blue and crystal violet dyes at neutral conditions by reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite.					
36109538	7	85	theme	as-fabricated	1256:1268	arg1	composite					1270:1278	the as-fabricated composite	1252:1278	the as-fabricated composite	1252:1278	Notably, the as-fabricated composite revealed an auspicious adsorbability towards the adsorptive removal of MB and CV, since the maximum adsorption capacity of MB and CV were 137 and 118 mg/g, respectively.					
36109538	8	86	theme	dyes	1545:1548	arg1	adsorption					1517:1526	the adsorption	1513:1526	the adsorption of both cationic dyes	1513:1548	Moreover, the isotherm and kinetic investigatins depicted that the adsorption of both cationic dyes fitted Langmuir and Pseudo 2nd order models, respectively.					
36109538	10	87	theme	real	1839:1842	arg1	water					1857:1861	real agricultural water	1839:1861	real agricultural water	1839:1861	More importantly, MMT-mAmCs efficiently adsorbed MB and CV from real agricultural water, Nile river water and wastewater samples at the neutral pH medium, reflecting its potentiality to be a superb reusable candidate for adsorptive removal cationic pollutants from their aquatic media.					
36109538	4	88	theme	potential	804:812	arg1	measurements					814:825	zeta potential measurements	799:825	zeta potential measurements	799:825	Interestingly, MMT-mAmCs composite carries high negative charges at a wide pH range from 4 to 11 as clarified from zeta potential measurements, asserting its suitability to adsorb the cationic contaminants.					
36109538	3	89	theme	amine-functionalized	486:505	arg1	composite					528:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	89	theme	amine-functionalized	486:505	arg1	green					553:557	a promising green	541:557	a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions	541:681	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	0	90	theme	crystal	48:54	arg1	violet					56:61	crystal violet	48:61	crystal violet	48:61	Efficient removal of noxious methylene blue and crystal violet dyes at neutral conditions by reusable montmorillonite/NiFe2O4@amine-functionalized chitosan composite.					
36109538	6	91	theme	ferromagnetic	1103:1115	arg1	behavior					1117:1124	the ferromagnetic behavior	1099:1124	the ferromagnetic behavior of the MMT-mAmCs composite	1099:1151	Furthermore, the ferromagnetic behavior of the MMT-mAmCs composite is additional merit to our adsorbent that provides facile, fast, and flawless separation.					
36109538	6	91	theme	ferromagnetic	1103:1115	arg1	merit					1167:1171	additional merit	1156:1171	additional merit to our adsorbent that provides facile, fast, and flawless separation	1156:1240	Furthermore, the ferromagnetic behavior of the MMT-mAmCs composite is additional merit to our adsorbent that provides facile, fast, and flawless separation.					
36109538	8	92	theme	Pseudo	1570:1575	arg1	models					1587:1592	Pseudo 2nd order models	1570:1592	Pseudo 2nd order models	1570:1592	Moreover, the isotherm and kinetic investigatins depicted that the adsorption of both cationic dyes fitted Langmuir and Pseudo 2nd order models, respectively.					
36109538	3	93	theme	neutral	664:670	arg1	conditions					672:681	neutral conditions	664:681	neutral conditions	664:681	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	1	94	theme	environment	236:246	arg1	jeopardy					171:178	The jeopardy	167:178	The jeopardy of the synthetic dyes effluents on human health and the environment	167:246	The jeopardy of the synthetic dyes effluents on human health and the environment has swiftly aggravated, threatening human survival.					
36109538	8	95	theme	order	1581:1585	arg1	models					1587:1592	Pseudo 2nd order models	1570:1592	Pseudo 2nd order models	1570:1592	Moreover, the isotherm and kinetic investigatins depicted that the adsorption of both cationic dyes fitted Langmuir and Pseudo 2nd order models, respectively.					
36109538	3	96	theme	adsorbent	559:567	arg1	composite					528:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite	453:536	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
36109538	3	96	theme	adsorbent	559:567	arg1	green					553:557	a promising green	541:557	a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions	541:681	Thereby, our investigation aimed to fabricate montmorillonite/magnetic NiFe2O4@amine-functionalized chitosan (MMT-mAmCs) composite as a promising green adsorbent to remove the cationic methylene blue (MB) and crystal violet (CV) dyes from the wastewater in neutral conditions.					
35580460	0	0	theme	flexible	97:104	arg1	sensors					106:112	self-healing hydrogels' flexible sensors	73:112	self-healing hydrogels' flexible sensors	73:112	Fabrication of Janus-type nanocomposites from cellulose nanocrystals for self-healing hydrogels' flexible sensors.					
35580460	6	1	theme	electronic	924:933	arg1	sensing					935:941	wearable electronic sensing	915:941	wearable electronic sensing	915:941	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	1	2	theme	solid	186:190	arg1	surfactants					192:202	functional solid surfactants	175:202	functional solid surfactants	175:202	Janus bio-nanomaterials have great application potential in functional solid surfactants, probes and flexible sensors.					
35580460	6	3	theme	wearable	915:922	arg1	sensing					935:941	wearable electronic sensing	915:941	wearable electronic sensing	915:941	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	3	4	theme	Janus	541:545	arg1	CNCs					535:538	CNCs	535:538	CNCs (Janus CNCs-PPy /PDA (JCNs))	535:567	The asymmetric functionalism of Janus nanorods was realized by asymmetrically grafting polypyrrole (PPy) and polydopamine (PDA) onto different sides of CNCs (Janus CNCs-PPy /PDA (JCNs)).					
35580460	3	4	theme	Janus	541:545	arg1	JCNs					562:565	JCNs	562:565	JCNs	562:565	The asymmetric functionalism of Janus nanorods was realized by asymmetrically grafting polypyrrole (PPy) and polydopamine (PDA) onto different sides of CNCs (Janus CNCs-PPy /PDA (JCNs)).					
35580460	3	4	theme	Janus	541:545	arg1	/PDA					556:559	Janus CNCs-PPy /PDA	541:559	Janus CNCs-PPy /PDA (JCNs)	541:566	The asymmetric functionalism of Janus nanorods was realized by asymmetrically grafting polypyrrole (PPy) and polydopamine (PDA) onto different sides of CNCs (Janus CNCs-PPy /PDA (JCNs)).					
35580460	1	5	contain	have	139:142	arg2	potential					162:170	great application potential	144:170	great application potential	144:170	Janus bio-nanomaterials have great application potential in functional solid surfactants, probes and flexible sensors.					
35580460	1	5	contain	have	139:142	arg1	bio-nanomaterials					121:137	Janus bio-nanomaterials	115:137	Janus bio-nanomaterials	115:137	Janus bio-nanomaterials have great application potential in functional solid surfactants, probes and flexible sensors.					
35580460	4	6	theme	nanocomposite	616:628	arg1	hydrogels					630:638	self-healing nanocomposite hydrogels	603:638	self-healing nanocomposite hydrogels	603:638	JCNs was successfully applied to self-healing nanocomposite hydrogels and further applied to the development of flexible sensors.					
35580460	3	7	theme	Janus	415:419	arg1	nanorods					421:428	Janus nanorods	415:428	Janus nanorods	415:428	The asymmetric functionalism of Janus nanorods was realized by asymmetrically grafting polypyrrole (PPy) and polydopamine (PDA) onto different sides of CNCs (Janus CNCs-PPy /PDA (JCNs)).					
35580460	4	8	theme	self-healing	603:614	arg1	hydrogels					630:638	self-healing nanocomposite hydrogels	603:638	self-healing nanocomposite hydrogels	603:638	JCNs was successfully applied to self-healing nanocomposite hydrogels and further applied to the development of flexible sensors.					
35580460	3	9	theme	asymmetric	387:396	arg1	functionalism					398:410	The asymmetric functionalism	383:410	The asymmetric functionalism of Janus nanorods	383:428	The asymmetric functionalism of Janus nanorods was realized by asymmetrically grafting polypyrrole (PPy) and polydopamine (PDA) onto different sides of CNCs (Janus CNCs-PPy /PDA (JCNs)).					
35580460	4	10	theme	sensors	691:697	arg1	development					667:677	the development	663:677	the development of flexible sensors	663:697	JCNs was successfully applied to self-healing nanocomposite hydrogels and further applied to the development of flexible sensors.					
35580460	4	11	theme	flexible	682:689	arg1	sensors					691:697	flexible sensors	682:697	flexible sensors	682:697	JCNs was successfully applied to self-healing nanocomposite hydrogels and further applied to the development of flexible sensors.					
35580460	6	12	theme	human	971:975	arg1	movement					977:984	human movement	971:984	human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms)	971:1072	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	2	13	theme	emulsion	357:364	arg1	method					375:380	Pickering emulsion template method	347:380	Pickering emulsion template method	347:380	In this manuscript, the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials were prepared by Pickering emulsion template method.					
35580460	6	14	theme	response	1051:1058	arg1	260 ms					1066:1071	260 ms	1066:1071	260 ms	1066:1071	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	6	14	theme	response	1051:1058	arg1	time					1060:1063	fast response time	1046:1063	fast response time (260 ms)	1046:1072	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	2	15	theme	Pickering	347:355	arg1	method					375:380	Pickering emulsion template method	347:380	Pickering emulsion template method	347:380	In this manuscript, the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials were prepared by Pickering emulsion template method.					
35580460	1	16	theme	flexible	216:223	arg1	sensors					225:231	flexible sensors	216:231	flexible sensors	216:231	Janus bio-nanomaterials have great application potential in functional solid surfactants, probes and flexible sensors.					
35580460	3	17	theme	nanorods	421:428	arg1	functionalism					398:410	The asymmetric functionalism	383:410	The asymmetric functionalism of Janus nanorods	383:428	The asymmetric functionalism of Janus nanorods was realized by asymmetrically grafting polypyrrole (PPy) and polydopamine (PDA) onto different sides of CNCs (Janus CNCs-PPy /PDA (JCNs)).					
35580460	6	18	theme	due	986:988	arg1	movement					977:984	human movement	971:984	human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms)	971:1072	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	5	19	theme	hydrogels	745:753	arg1	efficiency					717:726	The self-healing efficiency	700:726	The self-healing efficiency of nanocomposite hydrogels	700:753	The self-healing efficiency of nanocomposite hydrogels was 87.2%, and the stress and strain reached 3.50 MPa and 453.45%, respectively.					
35580460	5	19	theme	hydrogels	745:753	arg1	%					763:763	87.2%	759:763	87.2%	759:763	The self-healing efficiency of nanocomposite hydrogels was 87.2%, and the stress and strain reached 3.50 MPa and 453.45%, respectively.					
35580460	1	20	theme	Janus	115:119	arg1	bio-nanomaterials					121:137	Janus bio-nanomaterials	115:137	Janus bio-nanomaterials	115:137	Janus bio-nanomaterials have great application potential in functional solid surfactants, probes and flexible sensors.					
35580460	0	21	theme	Janus-type	15:24	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of Janus-type	0:24	Fabrication of Janus-type nanocomposites from cellulose nanocrystals for self-healing hydrogels' flexible sensors.					
35580460	6	22	theme	movement	977:984	arg1	monitoring					957:966	real-time monitoring	947:966	real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms)	947:1072	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	0	23	theme	cellulose	46:54	arg1	nanocrystals					56:67	cellulose nanocrystals	46:67	cellulose nanocrystals for self-healing hydrogels' flexible sensors	46:112	Fabrication of Janus-type nanocomposites from cellulose nanocrystals for self-healing hydrogels' flexible sensors.					
35580460	3	24	theme	CNCs	535:538	arg1	sides					526:530	different sides	516:530	different sides of CNCs (Janus CNCs-PPy /PDA (JCNs))	516:567	The asymmetric functionalism of Janus nanorods was realized by asymmetrically grafting polypyrrole (PPy) and polydopamine (PDA) onto different sides of CNCs (Janus CNCs-PPy /PDA (JCNs)).					
35580460	2	25	theme	sustainable	258:268	arg1	CNCs-type					305:313	CNCs-type	305:313	CNCs-type	305:313	In this manuscript, the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials were prepared by Pickering emulsion template method.					
35580460	2	25	theme	sustainable	258:268	arg1	nanocrystals-type					286:302	the sustainable Janus cellulose nanocrystals-type	254:302	the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials	254:328	In this manuscript, the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials were prepared by Pickering emulsion template method.					
35580460	6	26	theme	fast	1046:1049	arg1	260 ms					1066:1071	260 ms	1066:1071	260 ms	1066:1071	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	6	26	theme	fast	1046:1049	arg1	time					1060:1063	fast response time	1046:1063	fast response time (260 ms)	1046:1072	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	5	27	theme	self-healing	704:715	arg1	efficiency					717:726	The self-healing efficiency	700:726	The self-healing efficiency of nanocomposite hydrogels	700:753	The self-healing efficiency of nanocomposite hydrogels was 87.2%, and the stress and strain reached 3.50 MPa and 453.45%, respectively.					
35580460	5	27	theme	self-healing	704:715	arg1	%					763:763	87.2%	759:763	87.2%	759:763	The self-healing efficiency of nanocomposite hydrogels was 87.2%, and the stress and strain reached 3.50 MPa and 453.45%, respectively.					
35580460	6	28	theme	real-time	947:955	arg1	monitoring					957:966	real-time monitoring	947:966	real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms)	947:1072	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	6	29	theme	flexible	860:867	arg1	sensors					869:875	flexible sensors	860:875	flexible sensors	860:875	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	1	30	theme	great	144:148	arg1	potential					162:170	great application potential	144:170	great application potential	144:170	Janus bio-nanomaterials have great application potential in functional solid surfactants, probes and flexible sensors.					
35580460	1	31	theme	application	150:160	arg1	potential					162:170	great application potential	144:170	great application potential	144:170	Janus bio-nanomaterials have great application potential in functional solid surfactants, probes and flexible sensors.					
35580460	6	32	theme	high	999:1002	arg1	sensitivity					1004:1014	their high sensitivity	993:1014	their high sensitivity (gauge factor (GF) = 9.9)	993:1040	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	6	32	theme	high	999:1002	arg1	GF					1031:1032	gauge factor (GF) = 9.9	1017:1039	gauge factor (GF) = 9.9	1017:1039	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	5	33	theme	nanocomposite	731:743	arg1	hydrogels					745:753	nanocomposite hydrogels	731:753	nanocomposite hydrogels	731:753	The self-healing efficiency of nanocomposite hydrogels was 87.2%, and the stress and strain reached 3.50 MPa and 453.45%, respectively.					
35580460	2	34	theme	nanocrystals-type	286:302	arg1	nanomaterials					316:328	the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials	254:328	the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials	254:328	In this manuscript, the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials were prepared by Pickering emulsion template method.					
35580460	2	35	theme	template	366:373	arg1	method					375:380	Pickering emulsion template method	347:380	Pickering emulsion template method	347:380	In this manuscript, the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials were prepared by Pickering emulsion template method.					
35580460	3	36	theme	different	516:524	arg1	sides					526:530	different sides	516:530	different sides of CNCs (Janus CNCs-PPy /PDA (JCNs))	516:567	The asymmetric functionalism of Janus nanorods was realized by asymmetrically grafting polypyrrole (PPy) and polydopamine (PDA) onto different sides of CNCs (Janus CNCs-PPy /PDA (JCNs)).					
35580460	6	37	used	used	894:897	arg2	sensors					869:875	flexible sensors	860:875	flexible sensors	860:875	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	2	38	theme	cellulose	276:284	arg1	CNCs-type					305:313	CNCs-type	305:313	CNCs-type	305:313	In this manuscript, the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials were prepared by Pickering emulsion template method.					
35580460	2	38	theme	cellulose	276:284	arg1	nanocrystals-type					286:302	the sustainable Janus cellulose nanocrystals-type	254:302	the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials	254:328	In this manuscript, the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials were prepared by Pickering emulsion template method.					
35580460	3	39	theme	CNCs-PPy	547:554	arg1	CNCs					535:538	CNCs	535:538	CNCs (Janus CNCs-PPy /PDA (JCNs))	535:567	The asymmetric functionalism of Janus nanorods was realized by asymmetrically grafting polypyrrole (PPy) and polydopamine (PDA) onto different sides of CNCs (Janus CNCs-PPy /PDA (JCNs)).					
35580460	3	39	theme	CNCs-PPy	547:554	arg1	JCNs					562:565	JCNs	562:565	JCNs	562:565	The asymmetric functionalism of Janus nanorods was realized by asymmetrically grafting polypyrrole (PPy) and polydopamine (PDA) onto different sides of CNCs (Janus CNCs-PPy /PDA (JCNs)).					
35580460	3	39	theme	CNCs-PPy	547:554	arg1	/PDA					556:559	Janus CNCs-PPy /PDA	541:559	Janus CNCs-PPy /PDA (JCNs)	541:566	The asymmetric functionalism of Janus nanorods was realized by asymmetrically grafting polypyrrole (PPy) and polydopamine (PDA) onto different sides of CNCs (Janus CNCs-PPy /PDA (JCNs)).					
35580460	6	40	theme	factor	1023:1028	arg1	sensitivity					1004:1014	their high sensitivity	993:1014	their high sensitivity (gauge factor (GF) = 9.9)	993:1040	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	6	40	theme	factor	1023:1028	arg1	GF					1031:1032	gauge factor (GF) = 9.9	1017:1039	gauge factor (GF) = 9.9	1017:1039	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	0	41	theme	self-healing	73:84	arg1	sensors					106:112	self-healing hydrogels' flexible sensors	73:112	self-healing hydrogels' flexible sensors	73:112	Fabrication of Janus-type nanocomposites from cellulose nanocrystals for self-healing hydrogels' flexible sensors.					
35580460	6	42	theme	sensing	935:941	arg1	field					906:910	the field	902:910	the field of wearable electronic sensing	902:941	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	2	43	theme	Janus	270:274	arg1	CNCs-type					305:313	CNCs-type	305:313	CNCs-type	305:313	In this manuscript, the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials were prepared by Pickering emulsion template method.					
35580460	2	43	theme	Janus	270:274	arg1	nanocrystals-type					286:302	the sustainable Janus cellulose nanocrystals-type	254:302	the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials	254:328	In this manuscript, the sustainable Janus cellulose nanocrystals-type (CNCs-type) nanomaterials were prepared by Pickering emulsion template method.					
35580460	1	44	theme	functional	175:184	arg1	surfactants					192:202	functional solid surfactants	175:202	functional solid surfactants	175:202	Janus bio-nanomaterials have great application potential in functional solid surfactants, probes and flexible sensors.					
35580460	6	45	theme	gauge	1017:1021	arg1	sensitivity					1004:1014	their high sensitivity	993:1014	their high sensitivity (gauge factor (GF) = 9.9)	993:1040	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35580460	6	45	theme	gauge	1017:1021	arg1	GF					1031:1032	gauge factor (GF) = 9.9	1017:1039	gauge factor (GF) = 9.9	1017:1039	It is worth noting that flexible sensors have been widely used in the field of wearable electronic sensing for real-time monitoring of human movement due to their high sensitivity (gauge factor (GF) = 9.9) and fast response time (260 ms).					
35483840	2	0	theme	quantum	482:488	arg1	dots					490:493	graphene quantum dots	473:493	graphene quantum dots	473:493	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	7	1	theme	nanocomposite	1207:1219	arg1	utility					1171:1177	further utility	1163:1177	further utility of novel CS-GQDs caged drug nanocomposite for preclinical investigations	1163:1250	These findings justify further utility of novel CS-GQDs caged drug nanocomposite for preclinical investigations.					
35483840	1	2	theme	therapeutic	280:290	arg1	applications					292:303	clinical and therapeutic applications	267:303	clinical and therapeutic applications	267:303	The designing of highly efficient and biocompatible nanocomposites with multifunctional delivery and tracking characteristics is noteworthy for clinical and therapeutic applications.					
35483840	2	3	theme	graphene	473:480	arg1	dots					490:493	graphene quantum dots	473:493	graphene quantum dots	473:493	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	3	4	theme	particle	683:690	arg1	studies					717:723	extensive spectral, morphological, band-gap, particle size, and zeta potential studies	638:723	studies	717:723	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	3	4	theme	particle	683:690	arg1	size					692:695	particle size	683:695	particle size	683:695	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	4	5	theme	in	861:862	arg1	bio-imaging					870:880	in vitro bio-imaging	861:880	in vitro bio-imaging against human corneal epithelial (HCE) cells	861:925	The regaining of PL not only confirmed LP delivery, but also facilitated intercellular tracking through in vitro bio-imaging against human corneal epithelial (HCE) cells.					
35483840	1	6	theme	biocompatible	161:173	arg1	nanocomposites					175:188	highly efficient and biocompatible nanocomposites	140:188	highly efficient and biocompatible nanocomposites	140:188	The designing of highly efficient and biocompatible nanocomposites with multifunctional delivery and tracking characteristics is noteworthy for clinical and therapeutic applications.					
35483840	2	7	with	proof-of-concept	328:343	arg1	dots					490:493	graphene quantum dots	473:493	graphene quantum dots	473:493	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	2	7	with	proof-of-concept	328:343	arg1	CS					467:468	CS	467:468	CS	467:468	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	2	7	with	proof-of-concept	328:343	arg1	chitosan					457:464	novel chitosan	451:464	novel chitosan (CS)	451:469	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	2	7	with	proof-of-concept	328:343	arg1	GQD					496:498	GQD	496:498	GQD	496:498	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	0	8	theme	enzymatic	95:103	arg1	response					113:120	enzymatic stimuli response	95:120	enzymatic stimuli response	95:120	Novel chitosan - graphene quantum dots composite for therapeutic delivery and tracking through enzymatic stimuli response.					
35483840	5	9	theme	biocompatibility	951:966	arg1	assays					968:973	The AO/EB staining and biocompatibility assays	928:973	The AO/EB staining and biocompatibility assays	928:973	The AO/EB staining and biocompatibility assays further proved excellent cell viability of >80%.					
35483840	7	10	theme	drug	1202:1205	arg1	nanocomposite					1207:1219	novel CS-GQDs caged drug nanocomposite	1182:1219	novel CS-GQDs caged drug nanocomposite	1182:1219	These findings justify further utility of novel CS-GQDs caged drug nanocomposite for preclinical investigations.					
35483840	7	11	theme	novel	1182:1186	arg1	nanocomposite					1207:1219	novel CS-GQDs caged drug nanocomposite	1182:1219	novel CS-GQDs caged drug nanocomposite	1182:1219	These findings justify further utility of novel CS-GQDs caged drug nanocomposite for preclinical investigations.					
35483840	3	12	dep	spectral	648:655	arg1	morphological					658:670	morphological	658:670	morphological	658:670	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	4	13	theme	intercellular	830:842	arg1	tracking					844:851	intercellular tracking	830:851	intercellular tracking	830:851	The regaining of PL not only confirmed LP delivery, but also facilitated intercellular tracking through in vitro bio-imaging against human corneal epithelial (HCE) cells.					
35483840	6	14	theme	oxidative	1079:1087	arg1	injury					1089:1094	oxidative injury	1079:1094	oxidative injury induced by 800 μM hydrogen peroxide (H2O2)	1079:1137	The successfully delivered LP protected HCE cells from oxidative injury induced by 800 μM hydrogen peroxide (H2O2).					
35483840	4	15	theme	PL	774:775	arg1	regaining					761:769	The regaining	757:769	The regaining of PL	757:775	The regaining of PL not only confirmed LP delivery, but also facilitated intercellular tracking through in vitro bio-imaging against human corneal epithelial (HCE) cells.					
35483840	4	16	theme	HCE	916:918	arg1	cells					921:925	human corneal epithelial (HCE) cells	890:925	human corneal epithelial (HCE) cells	890:925	The regaining of PL not only confirmed LP delivery, but also facilitated intercellular tracking through in vitro bio-imaging against human corneal epithelial (HCE) cells.					
35483840	0	17	theme	stimuli	105:111	arg1	response					113:120	enzymatic stimuli response	95:120	enzymatic stimuli response	95:120	Novel chitosan - graphene quantum dots composite for therapeutic delivery and tracking through enzymatic stimuli response.					
35483840	3	18	theme	zeta	702:705	arg1	potential					707:715	zeta potential	702:715	zeta potential	702:715	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	3	18	theme	zeta	702:705	arg1	studies					717:723	extensive spectral, morphological, band-gap, particle size, and zeta potential studies	638:723	studies	717:723	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	0	19	theme	Novel	0:4	arg1	chitosan					6:13	Novel chitosan	0:13	Novel chitosan - graphene quantum dots composite for therapeutic delivery and tracking through enzymatic stimuli response.	0:121	Novel chitosan - graphene quantum dots composite for therapeutic delivery and tracking through enzymatic stimuli response.					
35483840	2	20	theme	enzymatic	411:419	arg1	stimulus					421:428	an enzymatic stimulus	408:428	an enzymatic stimulus	408:428	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	1	21	theme	multifunctional	195:209	arg1	delivery					211:218	multifunctional delivery	195:218	multifunctional delivery	195:218	The designing of highly efficient and biocompatible nanocomposites with multifunctional delivery and tracking characteristics is noteworthy for clinical and therapeutic applications.					
35483840	2	22	theme	novel	451:455	arg1	CS					467:468	CS	467:468	CS	467:468	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	2	22	theme	novel	451:455	arg1	chitosan					457:464	novel chitosan	451:464	novel chitosan (CS)	451:469	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	2	22	theme	novel	451:455	arg1	GQD					496:498	GQD	496:498	GQD	496:498	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	5	23	theme	AO/EB	932:936	arg1	staining					938:945	AO/EB staining	932:945	AO/EB staining	932:945	The AO/EB staining and biocompatibility assays further proved excellent cell viability of >80%.					
35483840	6	24	theme	HCE	1064:1066	arg1	cells					1068:1072	HCE cells	1064:1072	HCE cells	1064:1072	The successfully delivered LP protected HCE cells from oxidative injury induced by 800 μM hydrogen peroxide (H2O2).					
35483840	0	25	theme	graphene	17:24	arg1	dots					34:37	graphene quantum dots	17:37	graphene quantum dots	17:37	Novel chitosan - graphene quantum dots composite for therapeutic delivery and tracking through enzymatic stimuli response.					
35483840	4	26	theme	LP	796:797	arg1	delivery					799:806	LP delivery	796:806	LP delivery	796:806	The regaining of PL not only confirmed LP delivery, but also facilitated intercellular tracking through in vitro bio-imaging against human corneal epithelial (HCE) cells.					
35483840	5	27	theme	staining	938:945	arg1	assays					968:973	The AO/EB staining and biocompatibility assays	928:973	The AO/EB staining and biocompatibility assays	928:973	The AO/EB staining and biocompatibility assays further proved excellent cell viability of >80%.					
35483840	7	28	theme	further	1163:1169	arg1	utility					1171:1177	further utility	1163:1177	further utility of novel CS-GQDs caged drug nanocomposite for preclinical investigations	1163:1250	These findings justify further utility of novel CS-GQDs caged drug nanocomposite for preclinical investigations.					
35483840	2	29	theme	photoluminescence	537:553	arg1	phenomenon					560:569	reverse switching photoluminescence (PL) phenomenon	519:569	reverse switching photoluminescence (PL) phenomenon	519:569	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	3	30	theme	spectral	648:655	arg1	potential					707:715	zeta potential	702:715	zeta potential	702:715	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	3	30	theme	spectral	648:655	arg1	band-gap					673:680	band-gap	673:680	band-gap	673:680	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	3	30	theme	spectral	648:655	arg1	studies					717:723	extensive spectral, morphological, band-gap, particle size, and zeta potential studies	638:723	studies	717:723	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	3	30	theme	spectral	648:655	arg1	size					692:695	particle size	683:695	particle size	683:695	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	2	31	dep	report	317:322	arg1	nanocomposite					501:513	nanocomposite	501:513	report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon	317:569	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	1	32	theme	tracking	224:231	arg1	characteristics					233:247	tracking characteristics	224:247	tracking characteristics	224:247	The designing of highly efficient and biocompatible nanocomposites with multifunctional delivery and tracking characteristics is noteworthy for clinical and therapeutic applications.					
35483840	3	33	theme	cytotoxicity	736:747	arg1	assays					749:754	cytotoxicity assays	736:754	extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays	638:754	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	2	34	theme	switching	527:535	arg1	photoluminescence					537:553	reverse switching photoluminescence	519:553	reverse switching photoluminescence (PL) phenomenon	519:569	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	2	34	theme	switching	527:535	arg1	PL					556:557	PL	556:557	PL	556:557	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	3	35	theme	caged	579:583	arg1	nanocomposite					593:605	The LP caged CS-GQDs nanocomposite	572:605	The LP caged CS-GQDs nanocomposite	572:605	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	6	36	theme	delivered	1041:1049	arg1	LP					1051:1052	The successfully delivered LP	1024:1052	The successfully delivered LP	1024:1052	The successfully delivered LP protected HCE cells from oxidative injury induced by 800 μM hydrogen peroxide (H2O2).					
35483840	0	37	theme	quantum	26:32	arg1	dots					34:37	graphene quantum dots	17:37	graphene quantum dots	17:37	Novel chitosan - graphene quantum dots composite for therapeutic delivery and tracking through enzymatic stimuli response.					
35483840	2	38	theme	reverse	519:525	arg1	photoluminescence					537:553	reverse switching photoluminescence	519:553	reverse switching photoluminescence (PL) phenomenon	519:569	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	2	38	theme	reverse	519:525	arg1	PL					556:557	PL	556:557	PL	556:557	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	3	39	theme	CS-GQDs	585:591	arg1	nanocomposite					593:605	The LP caged CS-GQDs nanocomposite	572:605	The LP caged CS-GQDs nanocomposite	572:605	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	7	40	theme	CS-GQDs	1188:1194	arg1	nanocomposite					1207:1219	novel CS-GQDs caged drug nanocomposite	1182:1219	novel CS-GQDs caged drug nanocomposite	1182:1219	These findings justify further utility of novel CS-GQDs caged drug nanocomposite for preclinical investigations.					
35483840	7	41	theme	caged	1196:1200	arg1	nanocomposite					1207:1219	novel CS-GQDs caged drug nanocomposite	1182:1219	novel CS-GQDs caged drug nanocomposite	1182:1219	These findings justify further utility of novel CS-GQDs caged drug nanocomposite for preclinical investigations.					
35483840	4	42	theme	corneal	896:902	arg1	cells					921:925	human corneal epithelial (HCE) cells	890:925	human corneal epithelial (HCE) cells	890:925	The regaining of PL not only confirmed LP delivery, but also facilitated intercellular tracking through in vitro bio-imaging against human corneal epithelial (HCE) cells.					
35483840	3	43	theme	extensive	638:646	arg1	potential					707:715	zeta potential	702:715	zeta potential	702:715	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	3	43	theme	extensive	638:646	arg1	band-gap					673:680	band-gap	673:680	band-gap	673:680	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	3	43	theme	extensive	638:646	arg1	studies					717:723	extensive spectral, morphological, band-gap, particle size, and zeta potential studies	638:723	studies	717:723	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	3	43	theme	extensive	638:646	arg1	size					692:695	particle size	683:695	particle size	683:695	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	4	44	dep	in	861:862	arg1	vitro					864:868	vitro	864:868	vitro	864:868	The regaining of PL not only confirmed LP delivery, but also facilitated intercellular tracking through in vitro bio-imaging against human corneal epithelial (HCE) cells.					
35483840	3	45	dep	studies	717:723	arg1	potential					707:715	zeta potential	702:715	zeta potential	702:715	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	3	45	dep	studies	717:723	arg1	studies					717:723	extensive spectral, morphological, band-gap, particle size, and zeta potential studies	638:723	studies	717:723	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	3	45	dep	studies	717:723	arg1	band-gap					673:680	band-gap	673:680	band-gap	673:680	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	3	45	dep	studies	717:723	arg1	size					692:695	particle size	683:695	particle size	683:695	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	4	46	theme	human	890:894	arg1	cells					921:925	human corneal epithelial (HCE) cells	890:925	human corneal epithelial (HCE) cells	890:925	The regaining of PL not only confirmed LP delivery, but also facilitated intercellular tracking through in vitro bio-imaging against human corneal epithelial (HCE) cells.					
35483840	3	47	theme	LP	576:577	arg1	nanocomposite					593:605	The LP caged CS-GQDs nanocomposite	572:605	The LP caged CS-GQDs nanocomposite	572:605	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	2	48	theme	drug	379:382	arg1	delivery					353:360	the delivery	349:360	the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz)	349:444	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	3	49	dep	along	725:729	arg1	with					731:734	with	731:734	with	731:734	The LP caged CS-GQDs nanocomposite was well characterized through extensive spectral, morphological, band-gap, particle size, and zeta potential studies along with cytotoxicity assays.					
35483840	5	50	theme	excellent	990:998	arg1	viability					1005:1013	excellent cell viability	990:1013	excellent cell viability of >80%	990:1021	The AO/EB staining and biocompatibility assays further proved excellent cell viability of >80%.					
35483840	5	51	theme	%	1021:1021	arg1	viability					1005:1013	excellent cell viability	990:1013	excellent cell viability of >80%	990:1021	The AO/EB staining and biocompatibility assays further proved excellent cell viability of >80%.					
35483840	2	52	theme	anti-glaucoma	365:377	arg1	drug					379:382	anti-glaucoma drug	365:382	anti-glaucoma drug	365:382	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	2	52	theme	anti-glaucoma	365:377	arg1	lysozyme					431:438	lysozyme	431:438	lysozyme (Lyz)	431:444	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	2	52	theme	anti-glaucoma	365:377	arg1	latanoprost					385:395	latanoprost	385:395	latanoprost (LP) under an enzymatic stimulus	385:428	Herein, we report the proof-of-concept for the delivery of anti-glaucoma drug, latanoprost (LP) under an enzymatic stimulus, lysozyme (Lyz) with novel chitosan (CS) - graphene quantum dots (GQD) nanocomposite via reverse switching photoluminescence (PL) phenomenon.					
35483840	4	53	theme	epithelial	904:913	arg1	cells					921:925	human corneal epithelial (HCE) cells	890:925	human corneal epithelial (HCE) cells	890:925	The regaining of PL not only confirmed LP delivery, but also facilitated intercellular tracking through in vitro bio-imaging against human corneal epithelial (HCE) cells.					
35483840	5	54	theme	cell	1000:1003	arg1	viability					1005:1013	excellent cell viability	990:1013	excellent cell viability of >80%	990:1021	The AO/EB staining and biocompatibility assays further proved excellent cell viability of >80%.					
35483840	6	55	theme	hydrogen	1114:1121	arg1	peroxide					1123:1130	hydrogen peroxide	1114:1130	hydrogen peroxide (H2O2)	1114:1137	The successfully delivered LP protected HCE cells from oxidative injury induced by 800 μM hydrogen peroxide (H2O2).					
35483840	6	55	theme	hydrogen	1114:1121	arg1	H2O2					1133:1136	H2O2	1133:1136	H2O2	1133:1136	The successfully delivered LP protected HCE cells from oxidative injury induced by 800 μM hydrogen peroxide (H2O2).					
35483840	7	56	theme	preclinical	1225:1235	arg1	investigations					1237:1250	preclinical investigations	1225:1250	preclinical investigations	1225:1250	These findings justify further utility of novel CS-GQDs caged drug nanocomposite for preclinical investigations.					
35483840	0	57	dep	chitosan	6:13	arg1	composite					39:47	composite	39:47	composite	39:47	Novel chitosan - graphene quantum dots composite for therapeutic delivery and tracking through enzymatic stimuli response.					
35483840	1	58	theme	clinical	267:274	arg1	applications					292:303	clinical and therapeutic applications	267:303	clinical and therapeutic applications	267:303	The designing of highly efficient and biocompatible nanocomposites with multifunctional delivery and tracking characteristics is noteworthy for clinical and therapeutic applications.					
35483840	0	59	theme	therapeutic	53:63	arg1	delivery					65:72	therapeutic delivery	53:72	therapeutic delivery	53:72	Novel chitosan - graphene quantum dots composite for therapeutic delivery and tracking through enzymatic stimuli response.					
35483840	1	60	theme	efficient	147:155	arg1	nanocomposites					175:188	highly efficient and biocompatible nanocomposites	140:188	highly efficient and biocompatible nanocomposites	140:188	The designing of highly efficient and biocompatible nanocomposites with multifunctional delivery and tracking characteristics is noteworthy for clinical and therapeutic applications.					
36283638	6	0	theme	rRNA	885:888	arg1	sequencing					890:899	16S rRNA sequencing	881:899	16S rRNA sequencing	881:899	16S rRNA sequencing and gas chromatography-mass spectrometry were used to detect the changes of gut microbiota and metabolites.					
36283638	10	1	theme	AMP-activated	1541:1553	arg1	kinases					1563:1569	GPS-1 activated AMP-activated protein kinases	1525:1569	GPS-1 activated AMP-activated protein kinases	1525:1569	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	10	1	theme	AMP-activated	1541:1553	arg1	carboxylase					1598:1608	acetyl-CoA carboxylase	1587:1608	phosphorylated acetyl-CoA carboxylase	1572:1608	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	7	2	theme	quantitative	1104:1115	arg1	reaction					1144:1151	quantitative real-time polymerase chain reaction	1104:1151	quantitative real-time polymerase chain reaction	1104:1151	The protein and mRNA levels of lipid synthesis-related genes were detected by Western blot and quantitative real-time polymerase chain reaction.					
36283638	9	3	theme	fatty	1418:1422	arg1	acids					1424:1428	short-chain fatty acids	1406:1428	short-chain fatty acids	1406:1428	GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine.					
36283638	10	4	from	expression	1623:1632	arg1	rats					1715:1718	T2DM rats	1710:1718	T2DM rats	1710:1718	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	3	5	theme	pectin	541:546	arg1	effect					473:478	the effect	469:478	the effect	469:478	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	3	5	theme	pectin	541:546	arg1	mechanism					498:506	the potential mechanism	484:506	the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats	484:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	10	6	theme	phosphorylated	1572:1585	arg1	kinases					1563:1569	GPS-1 activated AMP-activated protein kinases	1525:1569	GPS-1 activated AMP-activated protein kinases	1525:1569	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	10	6	theme	phosphorylated	1572:1585	arg1	carboxylase					1598:1608	acetyl-CoA carboxylase	1587:1608	phosphorylated acetyl-CoA carboxylase	1572:1608	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	3	7	theme	rhamnogalacturonan-I	511:530	arg1	GPS-1					549:553	GPS-1	549:553	GPS-1	549:553	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	3	7	theme	rhamnogalacturonan-I	511:530	arg1	pectin					541:546	rhamnogalacturonan-I enriched pectin	511:546	rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats	511:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	6	8	used	used	947:950	arg2	sequencing					890:899	16S rRNA sequencing	881:899	16S rRNA sequencing	881:899	16S rRNA sequencing and gas chromatography-mass spectrometry were used to detect the changes of gut microbiota and metabolites.					
36283638	6	8	used	used	947:950	arg2	spectrometry					929:940	gas chromatography-mass spectrometry	905:940	gas chromatography-mass spectrometry	905:940	16S rRNA sequencing and gas chromatography-mass spectrometry were used to detect the changes of gut microbiota and metabolites.					
36283638	8	9	theme	lipid	1247:1251	arg1	RESULTS					1154:1160	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation	1154:1264	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats	1154:1277	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	8	9	theme	lipid	1247:1251	arg1	accumulation					1253:1264	hepatic lipid accumulation	1239:1264	hepatic lipid accumulation	1239:1264	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	11	10	theme	gut	1831:1833	arg1	microbiota					1835:1844	gut microbiota	1831:1844	gut microbiota	1831:1844	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	2	11	theme	ginseng	309:315	arg1	component					296:304	the main active component	280:304	the main active component of ginseng	280:315	Polysaccharide is the main active component of ginseng, and has been proved to have hypoglycaemic and hypolipidaemic effects, but its mechanism remains unclear.					
36283638	2	11	theme	ginseng	309:315	arg1	Polysaccharide					262:275	Polysaccharide	262:275	Polysaccharide	262:275	Polysaccharide is the main active component of ginseng, and has been proved to have hypoglycaemic and hypolipidaemic effects, but its mechanism remains unclear.					
36283638	8	12	theme	weight	1190:1195	arg1	RESULTS					1154:1160	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation	1154:1264	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats	1154:1277	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	8	12	theme	weight	1190:1195	arg1	loss					1197:1200	weight loss	1190:1200	weight loss	1190:1200	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	7	13	theme	synthesis-related	1046:1062	arg1	genes					1064:1068	lipid synthesis-related genes	1040:1068	lipid synthesis-related genes	1040:1068	The protein and mRNA levels of lipid synthesis-related genes were detected by Western blot and quantitative real-time polymerase chain reaction.					
36283638	9	14	theme	glucagon-like	1461:1473	arg1	peptide-1					1475:1483	glucagon-like peptide-1	1461:1483	glucagon-like peptide-1	1461:1483	GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine.					
36283638	10	15	theme	element-binding	1655:1669	arg1	protein-1c					1671:1680	sterol regulatory element-binding protein-1c	1637:1680	sterol regulatory element-binding protein-1c	1637:1680	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	0	16	theme	AMPK	134:137	arg1	pathway					139:145	AMPK pathway	134:145	AMPK pathway	134:145	Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway.					
36283638	3	17	theme	lipid	580:584	arg1	metabolism					586:595	lipid metabolism	580:595	lipid metabolism in type 2 diabetes mellitus (T2DM) rats	580:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	6	18	theme	chromatography-mass	909:927	arg1	spectrometry					929:940	gas chromatography-mass spectrometry	905:940	gas chromatography-mass spectrometry	905:940	16S rRNA sequencing and gas chromatography-mass spectrometry were used to detect the changes of gut microbiota and metabolites.					
36283638	11	19	from	metabolism	1774:1783	arg1	rats					1793:1796	T2DM rats	1788:1796	T2DM rats	1788:1796	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	0	20	from	metabolism	76:85	arg1	rats					106:109	type 2 diabetic rats	90:109	type 2 diabetic rats	90:109	Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway.					
36283638	10	21	theme	sterol	1637:1642	arg1	protein-1c					1671:1680	sterol regulatory element-binding protein-1c	1637:1680	sterol regulatory element-binding protein-1c	1637:1680	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	7	22	theme	mRNA	1025:1028	arg1	levels					1030:1035	The protein and mRNA levels	1009:1035	The protein and mRNA levels of lipid synthesis-related genes	1009:1068	The protein and mRNA levels of lipid synthesis-related genes were detected by Western blot and quantitative real-time polymerase chain reaction.					
36283638	9	23	theme	tyrosine	1497:1504	arg1	tyrosine					1506:1513	peptide tyrosine tyrosine	1489:1513	peptide tyrosine tyrosine	1489:1513	GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine.					
36283638	8	24	theme	GPS-1	1301:1305	arg1	intervention					1307:1318	GPS-1 intervention	1301:1318	GPS-1 intervention	1301:1318	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	11	25	theme	T2DM	1788:1791	arg1	rats					1793:1796	T2DM rats	1788:1796	T2DM rats	1788:1796	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	7	26	theme	protein	1013:1019	arg1	levels					1030:1035	The protein and mRNA levels	1009:1035	The protein and mRNA levels of lipid synthesis-related genes	1009:1068	The protein and mRNA levels of lipid synthesis-related genes were detected by Western blot and quantitative real-time polymerase chain reaction.					
36283638	3	27	theme	enriched	532:539	arg1	GPS-1					549:553	GPS-1	549:553	GPS-1	549:553	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	3	27	theme	enriched	532:539	arg1	pectin					541:546	rhamnogalacturonan-I enriched pectin	511:546	rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats	511:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	6	28	theme	metabolites	996:1006	arg1	changes					966:972	the changes	962:972	the changes of gut microbiota and metabolites	962:1006	16S rRNA sequencing and gas chromatography-mass spectrometry were used to detect the changes of gut microbiota and metabolites.					
36283638	4	29	theme	METHODS	652:658	arg1	GPS-1					660:664	MATERIALS AND METHODS GPS-1	638:664	MATERIALS AND METHODS GPS-1	638:664	MATERIALS AND METHODS GPS-1 was prepared by water extraction, ion-exchange and gel chromatography.					
36283638	1	30	theme	ETHNOPHARMACOLOGICAL	148:167	arg1	Panax					179:183	ETHNOPHARMACOLOGICAL RELEVANCE Panax	148:183	ETHNOPHARMACOLOGICAL RELEVANCE Panax ginseng C. A. Meyer (Ginseng)	148:213	ETHNOPHARMACOLOGICAL RELEVANCE Panax ginseng C. A. Meyer (Ginseng) has traditionally been used to treat diabetes.					
36283638	6	31	theme	microbiota	981:990	arg1	changes					966:972	the changes	962:972	the changes of gut microbiota and metabolites	962:1006	16S rRNA sequencing and gas chromatography-mass spectrometry were used to detect the changes of gut microbiota and metabolites.					
36283638	8	32	dep	polyphagia	1166:1175	arg1	The					1162:1164	The	1162:1164	The	1162:1164	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	8	33	dep	RESULTS	1154:1160	arg1	hyperlipidaemia					1219:1233	hyperlipidaemia	1219:1233	hyperlipidaemia	1219:1233	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	8	33	dep	RESULTS	1154:1160	arg1	RESULTS					1154:1160	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation	1154:1264	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats	1154:1277	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	8	33	dep	RESULTS	1154:1160	arg1	loss					1197:1200	weight loss	1190:1200	weight loss	1190:1200	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	8	33	dep	RESULTS	1154:1160	arg1	polyphagia					1166:1175	polyphagia	1166:1175	polyphagia	1166:1175	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	8	33	dep	RESULTS	1154:1160	arg1	hyperglycaemia					1203:1216	hyperglycaemia	1203:1216	hyperglycaemia	1203:1216	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	8	33	dep	RESULTS	1154:1160	arg1	accumulation					1253:1264	hepatic lipid accumulation	1239:1264	hepatic lipid accumulation	1239:1264	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	8	33	dep	RESULTS	1154:1160	arg1	polydipsia					1178:1187	polydipsia	1178:1187	polydipsia	1178:1187	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	0	34	theme	diabetic	97:104	arg1	rats					106:109	type 2 diabetic rats	90:109	type 2 diabetic rats	90:109	Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway.					
36283638	2	35	theme	main	284:287	arg1	component					296:304	the main active component	280:304	the main active component of ginseng	280:315	Polysaccharide is the main active component of ginseng, and has been proved to have hypoglycaemic and hypolipidaemic effects, but its mechanism remains unclear.					
36283638	2	35	theme	main	284:287	arg1	Polysaccharide					262:275	Polysaccharide	262:275	Polysaccharide	262:275	Polysaccharide is the main active component of ginseng, and has been proved to have hypoglycaemic and hypolipidaemic effects, but its mechanism remains unclear.					
36283638	10	36	theme	synthases	1697:1705	arg1	expression					1623:1632	the expression	1619:1632	the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats	1619:1718	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	10	37	theme	fatty	1686:1690	arg1	synthases					1697:1705	fatty acid synthases	1686:1705	fatty acid synthases	1686:1705	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	0	38	theme	Rhamnogalacturonan-I	0:19	arg1	pectin					30:35	Rhamnogalacturonan-I enriched pectin	0:35	Rhamnogalacturonan-I enriched pectin from steamed ginseng	0:56	Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway.					
36283638	4	39	theme	gel	717:719	arg1	chromatography					721:734	gel chromatography	717:734	gel chromatography	717:734	MATERIALS AND METHODS GPS-1 was prepared by water extraction, ion-exchange and gel chromatography.					
36283638	3	40	from	ginseng	569:575	arg1	GPS-1					549:553	GPS-1	549:553	GPS-1	549:553	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	3	40	from	ginseng	569:575	arg1	pectin					541:546	rhamnogalacturonan-I enriched pectin	511:546	rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats	511:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	3	40	from	ginseng	569:575	arg1	effect					473:478	the effect	469:478	the effect	469:478	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	3	40	from	ginseng	569:575	arg1	metabolism					586:595	lipid metabolism	580:595	lipid metabolism in type 2 diabetes mellitus (T2DM) rats	580:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	3	40	from	ginseng	569:575	arg1	mechanism					498:506	the potential mechanism	484:506	the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats	484:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	5	41	theme	T2DM	815:818	arg1	models					824:829	T2DM rat models	815:829	T2DM rat models	815:829	High-glucose/high-fat diet combined with streptozotocin was used to establish T2DM rat models, and lipid levels in serum and liver were tested.					
36283638	9	42	theme	microbiota	1345:1354	arg1	composition					1356:1366	the gut microbiota composition	1337:1366	the gut microbiota composition of T2DM rats	1337:1379	GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine.					
36283638	10	43	theme	T2DM	1710:1713	arg1	rats					1715:1718	T2DM rats	1710:1718	T2DM rats	1710:1718	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	3	44	theme	STUDY	434:438	arg1	AIM					423:425	AIM	423:425	AIM OF THE STUDY	423:438	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	0	45	theme	steamed	42:48	arg1	ginseng					50:56	steamed ginseng	42:56	steamed ginseng	42:56	Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway.					
36283638	10	46	theme	activated	1531:1539	arg1	kinases					1563:1569	GPS-1 activated AMP-activated protein kinases	1525:1569	GPS-1 activated AMP-activated protein kinases	1525:1569	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	10	46	theme	activated	1531:1539	arg1	carboxylase					1598:1608	acetyl-CoA carboxylase	1587:1608	phosphorylated acetyl-CoA carboxylase	1572:1608	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	3	47	theme	mellitus	616:623	arg1	rats					632:635	type 2 diabetes mellitus (T2DM) rats	600:635	type 2 diabetes mellitus (T2DM) rats	600:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	11	48	theme	pathway	1893:1899	arg1	activation					1850:1859	activation	1850:1859	activation of AMP-activated protein kinase pathway	1850:1899	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	11	48	theme	pathway	1893:1899	arg1	regulation					1817:1826	the regulation	1813:1826	the regulation of gut microbiota	1813:1844	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	7	49	theme	polymerase	1127:1136	arg1	reaction					1144:1151	quantitative real-time polymerase chain reaction	1104:1151	quantitative real-time polymerase chain reaction	1104:1151	The protein and mRNA levels of lipid synthesis-related genes were detected by Western blot and quantitative real-time polymerase chain reaction.					
36283638	9	50	theme	rats	1376:1379	arg1	composition					1356:1366	the gut microbiota composition	1337:1366	the gut microbiota composition of T2DM rats	1337:1379	GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine.					
36283638	11	51	theme	lipid	1768:1772	arg1	metabolism					1774:1783	lipid metabolism	1768:1783	lipid metabolism in T2DM rats	1768:1796	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	11	52	theme	protein	1878:1884	arg1	kinase					1886:1891	AMP-activated protein kinase	1864:1891	AMP-activated protein kinase pathway	1864:1899	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	11	53	dep	CONCLUSIONS	1721:1731	arg1	effects					1748:1754	The regulation effects	1733:1754	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats	1721:1796	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	5	54	used	used	797:800	arg2	diet					759:762	High-glucose/high-fat diet	737:762	High-glucose/high-fat diet combined with streptozotocin	737:791	High-glucose/high-fat diet combined with streptozotocin was used to establish T2DM rat models, and lipid levels in serum and liver were tested.					
36283638	8	55	from	RESULTS	1154:1160	arg1	rats					1274:1277	T2DM rats	1269:1277	T2DM rats	1269:1277	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	11	56	theme	GPS-1	1759:1763	arg1	effects					1748:1754	The regulation effects	1733:1754	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats	1721:1796	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	3	57	theme	potential	488:496	arg1	mechanism					498:506	the potential mechanism	484:506	the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats	484:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	7	58	theme	real-time	1117:1125	arg1	reaction					1144:1151	quantitative real-time polymerase chain reaction	1104:1151	quantitative real-time polymerase chain reaction	1104:1151	The protein and mRNA levels of lipid synthesis-related genes were detected by Western blot and quantitative real-time polymerase chain reaction.					
36283638	3	59	dep	aimed	451:455	arg1	AIM					423:425	AIM	423:425	AIM OF THE STUDY	423:438	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	10	60	theme	protein	1555:1561	arg1	kinases					1563:1569	GPS-1 activated AMP-activated protein kinases	1525:1569	GPS-1 activated AMP-activated protein kinases	1525:1569	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	10	60	theme	protein	1555:1561	arg1	carboxylase					1598:1608	acetyl-CoA carboxylase	1587:1608	phosphorylated acetyl-CoA carboxylase	1572:1608	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	9	61	theme	short-chain	1406:1416	arg1	acids					1424:1428	short-chain fatty acids	1406:1428	short-chain fatty acids	1406:1428	GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine.					
36283638	6	62	theme	16S	881:883	arg1	sequencing					890:899	16S rRNA sequencing	881:899	16S rRNA sequencing	881:899	16S rRNA sequencing and gas chromatography-mass spectrometry were used to detect the changes of gut microbiota and metabolites.					
36283638	7	63	theme	Western	1087:1093	arg1	blot					1095:1098	Western blot	1087:1098	Western blot	1087:1098	The protein and mRNA levels of lipid synthesis-related genes were detected by Western blot and quantitative real-time polymerase chain reaction.					
36283638	2	64	theme	active	289:294	arg1	component					296:304	the main active component	280:304	the main active component of ginseng	280:315	Polysaccharide is the main active component of ginseng, and has been proved to have hypoglycaemic and hypolipidaemic effects, but its mechanism remains unclear.					
36283638	2	64	theme	active	289:294	arg1	Polysaccharide					262:275	Polysaccharide	262:275	Polysaccharide	262:275	Polysaccharide is the main active component of ginseng, and has been proved to have hypoglycaemic and hypolipidaemic effects, but its mechanism remains unclear.					
36283638	9	65	theme	acids	1424:1428	arg1	levels					1396:1401	the levels	1392:1401	the levels of short-chain fatty acids	1392:1428	GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine.					
36283638	10	66	theme	acetyl-CoA	1587:1596	arg1	kinases					1563:1569	GPS-1 activated AMP-activated protein kinases	1525:1569	GPS-1 activated AMP-activated protein kinases	1525:1569	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	10	66	theme	acetyl-CoA	1587:1596	arg1	carboxylase					1598:1608	acetyl-CoA carboxylase	1587:1608	phosphorylated acetyl-CoA carboxylase	1572:1608	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	3	67	from	mechanism	498:506	arg1	ginseng					569:575	steamed ginseng	561:575	steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats	561:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	4	68	theme	MATERIALS	638:646	arg1	GPS-1					660:664	MATERIALS AND METHODS GPS-1	638:664	MATERIALS AND METHODS GPS-1	638:664	MATERIALS AND METHODS GPS-1 was prepared by water extraction, ion-exchange and gel chromatography.					
36283638	5	69	theme	lipid	836:840	arg1	levels					842:847	lipid levels	836:847	lipid levels in serum and liver	836:866	High-glucose/high-fat diet combined with streptozotocin was used to establish T2DM rat models, and lipid levels in serum and liver were tested.					
36283638	7	70	theme	genes	1064:1068	arg1	levels					1030:1035	The protein and mRNA levels	1009:1035	The protein and mRNA levels of lipid synthesis-related genes	1009:1068	The protein and mRNA levels of lipid synthesis-related genes were detected by Western blot and quantitative real-time polymerase chain reaction.					
36283638	8	71	theme	hepatic	1239:1245	arg1	RESULTS					1154:1160	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation	1154:1264	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats	1154:1277	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	8	71	theme	hepatic	1239:1245	arg1	accumulation					1253:1264	hepatic lipid accumulation	1239:1264	hepatic lipid accumulation	1239:1264	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	11	72	theme	microbiota	1835:1844	arg1	activation					1850:1859	activation	1850:1859	activation of AMP-activated protein kinase pathway	1850:1899	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	11	72	theme	microbiota	1835:1844	arg1	regulation					1817:1826	the regulation	1813:1826	the regulation of gut microbiota	1813:1844	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	7	73	theme	lipid	1040:1044	arg1	genes					1064:1068	lipid synthesis-related genes	1040:1068	lipid synthesis-related genes	1040:1068	The protein and mRNA levels of lipid synthesis-related genes were detected by Western blot and quantitative real-time polymerase chain reaction.					
36283638	3	74	from	effect	473:478	arg1	ginseng					569:575	steamed ginseng	561:575	steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats	561:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	5	75	theme	rat	820:822	arg1	models					824:829	T2DM rat models	815:829	T2DM rat models	815:829	High-glucose/high-fat diet combined with streptozotocin was used to establish T2DM rat models, and lipid levels in serum and liver were tested.					
36283638	10	76	theme	protein-1c	1671:1680	arg1	expression					1623:1632	the expression	1619:1632	the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats	1619:1718	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	9	77	theme	peptide-1	1475:1483	arg1	secretion					1448:1456	the secretion	1444:1456	the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine	1444:1513	GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine.					
36283638	8	78	theme	T2DM	1269:1272	arg1	rats					1274:1277	T2DM rats	1269:1277	T2DM rats	1269:1277	RESULTS The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention.					
36283638	10	79	theme	regulatory	1644:1653	arg1	protein-1c					1671:1680	sterol regulatory element-binding protein-1c	1637:1680	sterol regulatory element-binding protein-1c	1637:1680	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	11	80	theme	AMP-activated	1864:1876	arg1	kinase					1886:1891	AMP-activated protein kinase	1864:1891	AMP-activated protein kinase pathway	1864:1899	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	3	81	theme	steamed	561:567	arg1	ginseng					569:575	steamed ginseng	561:575	steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats	561:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	9	82	theme	peptide	1489:1495	arg1	tyrosine					1506:1513	peptide tyrosine tyrosine	1489:1513	peptide tyrosine tyrosine	1489:1513	GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine.					
36283638	6	83	theme	gas	905:907	arg1	spectrometry					929:940	gas chromatography-mass spectrometry	905:940	gas chromatography-mass spectrometry	905:940	16S rRNA sequencing and gas chromatography-mass spectrometry were used to detect the changes of gut microbiota and metabolites.					
36283638	3	84	theme	type	600:603	arg1	T2DM					626:629	T2DM	626:629	T2DM	626:629	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	3	84	theme	type	600:603	arg1	mellitus					616:623	type 2 diabetes mellitus	600:623	type 2 diabetes mellitus (T2DM) rats	600:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	9	85	theme	tyrosine	1506:1513	arg1	secretion					1448:1456	the secretion	1444:1456	the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine	1444:1513	GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine.					
36283638	4	86	theme	water	682:686	arg1	extraction					688:697	water extraction	682:697	water extraction	682:697	MATERIALS AND METHODS GPS-1 was prepared by water extraction, ion-exchange and gel chromatography.					
36283638	5	87	theme	High-glucose/high-fat	737:757	arg1	diet					759:762	High-glucose/high-fat diet	737:762	High-glucose/high-fat diet combined with streptozotocin	737:791	High-glucose/high-fat diet combined with streptozotocin was used to establish T2DM rat models, and lipid levels in serum and liver were tested.					
36283638	5	88	from	levels	842:847	arg1	liver					862:866	liver	862:866	liver	862:866	High-glucose/high-fat diet combined with streptozotocin was used to establish T2DM rat models, and lipid levels in serum and liver were tested.					
36283638	5	88	from	levels	842:847	arg1	serum					852:856	serum	852:856	serum	852:856	High-glucose/high-fat diet combined with streptozotocin was used to establish T2DM rat models, and lipid levels in serum and liver were tested.					
36283638	1	89	theme	RELEVANCE	169:177	arg1	Panax					179:183	ETHNOPHARMACOLOGICAL RELEVANCE Panax	148:183	ETHNOPHARMACOLOGICAL RELEVANCE Panax ginseng C. A. Meyer (Ginseng)	148:213	ETHNOPHARMACOLOGICAL RELEVANCE Panax ginseng C. A. Meyer (Ginseng) has traditionally been used to treat diabetes.					
36283638	6	90	theme	gut	977:979	arg1	microbiota					981:990	gut microbiota	977:990	gut microbiota	977:990	16S rRNA sequencing and gas chromatography-mass spectrometry were used to detect the changes of gut microbiota and metabolites.					
36283638	3	91	from	metabolism	586:595	arg1	rats					632:635	type 2 diabetes mellitus (T2DM) rats	600:635	type 2 diabetes mellitus (T2DM) rats	600:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	0	92	theme	gut	115:117	arg1	microbiota					119:128	gut microbiota	115:128	gut microbiota	115:128	Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway.					
36283638	2	93	contain	have	341:344	arg1	component					296:304	the main active component	280:304	the main active component of ginseng	280:315	Polysaccharide is the main active component of ginseng, and has been proved to have hypoglycaemic and hypolipidaemic effects, but its mechanism remains unclear.					
36283638	2	93	contain	have	341:344	arg2	effects					379:385	hypoglycaemic and hypolipidaemic effects	346:385	hypoglycaemic and hypolipidaemic effects	346:385	Polysaccharide is the main active component of ginseng, and has been proved to have hypoglycaemic and hypolipidaemic effects, but its mechanism remains unclear.					
36283638	2	93	contain	have	341:344	arg1	Polysaccharide					262:275	Polysaccharide	262:275	Polysaccharide	262:275	Polysaccharide is the main active component of ginseng, and has been proved to have hypoglycaemic and hypolipidaemic effects, but its mechanism remains unclear.					
36283638	11	94	from	effects	1748:1754	arg1	metabolism					1774:1783	lipid metabolism	1768:1783	lipid metabolism in T2DM rats	1768:1796	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	0	95	from	ginseng	50:56	arg1	pectin					30:35	Rhamnogalacturonan-I enriched pectin	0:35	Rhamnogalacturonan-I enriched pectin from steamed ginseng	0:56	Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway.					
36283638	10	96	theme	acid	1692:1695	arg1	synthases					1697:1705	fatty acid synthases	1686:1705	fatty acid synthases	1686:1705	Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats.					
36283638	0	97	theme	enriched	21:28	arg1	pectin					30:35	Rhamnogalacturonan-I enriched pectin	0:35	Rhamnogalacturonan-I enriched pectin from steamed ginseng	0:56	Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway.					
36283638	2	98	theme	hypolipidaemic	364:377	arg1	effects					379:385	hypoglycaemic and hypolipidaemic effects	346:385	hypoglycaemic and hypolipidaemic effects	346:385	Polysaccharide is the main active component of ginseng, and has been proved to have hypoglycaemic and hypolipidaemic effects, but its mechanism remains unclear.					
36283638	9	99	theme	gut	1341:1343	arg1	composition					1356:1366	the gut microbiota composition	1337:1366	the gut microbiota composition of T2DM rats	1337:1379	GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine.					
36283638	11	100	theme	regulation	1737:1746	arg1	effects					1748:1754	The regulation effects	1733:1754	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats	1721:1796	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	7	101	theme	chain	1138:1142	arg1	reaction					1144:1151	quantitative real-time polymerase chain reaction	1104:1151	quantitative real-time polymerase chain reaction	1104:1151	The protein and mRNA levels of lipid synthesis-related genes were detected by Western blot and quantitative real-time polymerase chain reaction.					
36283638	3	102	theme	diabetes	607:614	arg1	T2DM					626:629	T2DM	626:629	T2DM	626:629	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	3	102	theme	diabetes	607:614	arg1	mellitus					616:623	type 2 diabetes mellitus	600:623	type 2 diabetes mellitus (T2DM) rats	600:635	AIM OF THE STUDY This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats.					
36283638	0	103	theme	lipid	70:74	arg1	metabolism					76:85	lipid metabolism	70:85	lipid metabolism in type 2 diabetic rats	70:109	Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway.					
36283638	9	104	theme	T2DM	1371:1374	arg1	rats					1376:1379	T2DM rats	1371:1379	T2DM rats	1371:1379	GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine.					
36283638	1	105	used	used	238:241	arg2	Panax					179:183	ETHNOPHARMACOLOGICAL RELEVANCE Panax	148:183	ETHNOPHARMACOLOGICAL RELEVANCE Panax ginseng C. A. Meyer (Ginseng)	148:213	ETHNOPHARMACOLOGICAL RELEVANCE Panax ginseng C. A. Meyer (Ginseng) has traditionally been used to treat diabetes.					
36283638	11	106	theme	kinase	1886:1891	arg1	pathway					1893:1899	AMP-activated protein kinase pathway	1864:1899	AMP-activated protein kinase pathway	1864:1899	CONCLUSIONS The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.					
36283638	2	107	theme	hypoglycaemic	346:358	arg1	effects					379:385	hypoglycaemic and hypolipidaemic effects	346:385	hypoglycaemic and hypolipidaemic effects	346:385	Polysaccharide is the main active component of ginseng, and has been proved to have hypoglycaemic and hypolipidaemic effects, but its mechanism remains unclear.					
35196217	11	0	theme	vaccine	2127:2133	arg1	development					2135:2145	vaccine development	2127:2145	vaccine development	2127:2145	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	7	1	theme	genome	1209:1214	arg1	comparisons					1216:1226	blast-based genome comparisons	1197:1226	blast-based genome comparisons	1197:1226	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	3	2	theme	different	652:660	arg1	serotypes					662:670	17 different serotypes	649:670	17 different serotypes	649:670	We aimed to complete this picture by sequencing the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform.					
35196217	7	3	theme	different	1401:1409	arg1	serotypes					1411:1419	the 18 different serotypes	1394:1419	the 18 different serotypes	1394:1419	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	10	4	theme	intra-	1928:1933	arg1	exchange					1952:1959	a dynamic intra- and interspecies exchange	1918:1959	a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer	1918:2021	Taken together with the presence of transposable elements surrounding these loci, we speculate a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer.					
35196217	3	5	theme	MinION	681:686	arg1	sequencer					688:696	the MinION sequencer	677:696	the MinION sequencer (Oxford Nanopore Technologies, ONT)	677:732	We aimed to complete this picture by sequencing the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform.					
35196217	2	6	theme	distinctive	319:329	arg1	LPS					351:353	LPS	351:353	LPS	351:353	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	2	6	theme	distinctive	319:329	arg1	lipopolysaccharide					331:348	distinctive lipopolysaccharide	319:348	distinctive lipopolysaccharide (LPS)	319:354	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	1	7	theme	family	195:200	arg1	Pasteurellaceae					202:216	the family Pasteurellaceae	191:216	the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide	191:292	Actinobacillus pleuropneumoniae is a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide.					
35196217	4	8	theme	field	801:805	arg1	isolates					807:814	two field isolates	797:814	two field isolates of serotypes 2 and 3 that were PacBio- and MinION-sequenced, respectively	797:888	We also included two field isolates of serotypes 2 and 3 that were PacBio- and MinION-sequenced, respectively.					
35196217	11	9	theme	virulence	2283:2291	arg1	genes					2293:2297	antibiotic resistance and virulence genes	2257:2297	genes	2293:2297	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	2	10	theme	capsular	360:367	arg1	compositions					375:386	capsular (CPS) compositions	360:386	capsular (CPS) compositions	360:386	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	3	11	theme	Nanopore	706:713	arg1	ONT					729:731	ONT	729:731	ONT	729:731	We aimed to complete this picture by sequencing the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform.					
35196217	3	11	theme	Nanopore	706:713	arg1	Technologies					715:726	Oxford Nanopore Technologies	699:726	Oxford Nanopore Technologies	699:726	We aimed to complete this picture by sequencing the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform.					
35196217	11	12	theme	comprehensive	2043:2055	arg1	analysis					2066:2073	our comprehensive genomics analysis	2039:2073	our comprehensive genomics analysis	2039:2073	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	7	13	theme	circular	1337:1344	arg1	genomes					1346:1352	the 26 circular genomes	1330:1352	the 26 circular genomes	1330:1352	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	11	14	from	pleuropneumoniae	2305:2320	arg1	evolution					2244:2252	the evolution	2240:2252	the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae	2240:2320	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	10	15	theme	virulence-related	1969:1985	arg1	factors					1987:1993	such virulence-related factors	1964:1993	such virulence-related factors	1964:1993	Taken together with the presence of transposable elements surrounding these loci, we speculate a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer.					
35196217	2	16	with	serotypes	304:312	arg1	compositions					375:386	capsular (CPS) compositions	360:386	capsular (CPS) compositions	360:386	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	2	16	with	serotypes	304:312	arg1	LPS					351:353	LPS	351:353	LPS	351:353	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	2	16	with	serotypes	304:312	arg1	lipopolysaccharide					331:348	distinctive lipopolysaccharide	319:348	distinctive lipopolysaccharide (LPS)	319:354	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	11	17	theme	useful	2084:2089	arg1	information					2091:2101	useful information	2084:2101	useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae	2084:2320	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	1	18	theme	Gram-negative	152:164	arg1	pleuropneumoniae					130:145	Actinobacillus pleuropneumoniae	115:145	Actinobacillus pleuropneumoniae	115:145	Actinobacillus pleuropneumoniae is a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide.					
35196217	1	18	theme	Gram-negative	152:164	arg1	bacterium					178:186	a Gram-negative, rod-shaped bacterium	150:186	a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide	150:292	Actinobacillus pleuropneumoniae is a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide.					
35196217	6	19	theme	circular	1153:1160	arg1	genomes					1162:1168	accurate circular genomes	1144:1168	accurate circular genomes	1144:1168	Both methods proved successful in obtaining accurate circular genomes with comparable qualities.					
35196217	11	20	theme	diagnostic	2107:2116	arg1	test					2118:2121	diagnostic test	2107:2121	diagnostic test	2107:2121	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	10	21	theme	gene	2009:2012	arg1	transfer					2014:2021	horizontal gene transfer	1998:2021	horizontal gene transfer	1998:2021	Taken together with the presence of transposable elements surrounding these loci, we speculate a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer.					
35196217	7	22	theme	blast-based	1197:1207	arg1	comparisons					1216:1226	blast-based genome comparisons	1197:1226	blast-based genome comparisons	1197:1226	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	11	23	theme	epidemiological	2180:2194	arg1	studies					2196:2202	whole-genome-based epidemiological studies	2161:2202	whole-genome-based epidemiological studies	2161:2202	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	9	24	theme	serotype-specific	1724:1740	arg1	traits					1742:1747	all those serotype-specific traits	1714:1747	all those serotype-specific traits	1714:1747	Of note, highly similar clusters encoding all those serotype-specific traits were also found in other pathogenic and commensal Actinobacillus species.					
35196217	0	25	theme	serotypes	69:77	arg1	genomes					45:51	26 complete circular genomes	24:51	26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae	24:112	Comparative genomics of 26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae.					
35196217	2	26	theme	field	534:538	arg1	strains					540:546	field strains	534:546	field strains of serotypes 1, 3, 7 and 8	534:573	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	1	27	theme	Actinobacillus	115:128	arg1	pleuropneumoniae					130:145	Actinobacillus pleuropneumoniae	115:145	Actinobacillus pleuropneumoniae	115:145	Actinobacillus pleuropneumoniae is a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide.					
35196217	1	27	theme	Actinobacillus	115:128	arg1	bacterium					178:186	a Gram-negative, rod-shaped bacterium	150:186	a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide	150:292	Actinobacillus pleuropneumoniae is a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide.					
35196217	0	28	theme	Actinobacillus	82:95	arg1	pleuropneumoniae					97:112	Actinobacillus pleuropneumoniae	82:112	Actinobacillus pleuropneumoniae	82:112	Comparative genomics of 26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae.					
35196217	5	29	theme	hybrid	1038:1043	arg1	assembly					1045:1052	a hybrid assembly	1036:1052	a hybrid assembly	1036:1052	Genome assemblies were performed following two different strategies, i.e. PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads.					
35196217	2	30	dep	serotypes	503:511	arg1	1					513:513	1	513:513	1	513:513	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	11	31	theme	evolution	2244:2252	arg1	surveillance					2224:2235	the surveillance	2220:2235	the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae	2220:2320	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	2	32	theme	circular	429:436	arg1	genomes					438:444	complete circular genomes	420:444	complete circular genomes	420:444	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	2	33	theme	reference	482:490	arg1	strains					492:498	the reference strains	478:498	the reference strains of serotypes 1	478:513	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	11	34	theme	antibiotic	2257:2266	arg1	resistance					2268:2277	antibiotic resistance and virulence genes	2257:2297	resistance	2268:2277	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	0	35	theme	Comparative	0:10	arg1	genomics					12:19	Comparative genomics	0:19	Comparative genomics of 26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae	0:112	Comparative genomics of 26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae.					
35196217	7	36	theme	multi-locus	1290:1300	arg1	cgMLST					1319:1324	cgMLST	1319:1324	cgMLST	1319:1324	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	7	36	theme	multi-locus	1290:1300	arg1	typing					1311:1316	multi-locus sequence typing	1290:1316	multi-locus sequence typing (cgMLST)	1290:1325	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	11	37	theme	whole-genome-based	2161:2178	arg1	studies					2196:2202	whole-genome-based epidemiological studies	2161:2202	whole-genome-based epidemiological studies	2161:2202	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	9	38	located	found	1759:1763	arg1	species					1814:1820	other pathogenic and commensal Actinobacillus species	1768:1820	other pathogenic and commensal Actinobacillus species	1768:1820	Of note, highly similar clusters encoding all those serotype-specific traits were also found in other pathogenic and commensal Actinobacillus species.					
35196217	9	38	located	found	1759:1763	arg2	clusters					1696:1703	highly similar clusters	1681:1703	highly similar clusters encoding all those serotype-specific traits	1681:1747	Of note, highly similar clusters encoding all those serotype-specific traits were also found in other pathogenic and commensal Actinobacillus species.					
35196217	9	39	theme	Actinobacillus	1799:1812	arg1	species					1814:1820	other pathogenic and commensal Actinobacillus species	1768:1820	other pathogenic and commensal Actinobacillus species	1768:1820	Of note, highly similar clusters encoding all those serotype-specific traits were also found in other pathogenic and commensal Actinobacillus species.					
35196217	0	40	theme	complete	27:34	arg1	genomes					45:51	26 complete circular genomes	24:51	26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae	24:112	Comparative genomics of 26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae.					
35196217	7	41	theme	RTX-toxin	1477:1485	arg1	clusters					1487:1494	RTX-toxin clusters	1477:1494	RTX-toxin clusters	1477:1494	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	7	41	theme	RTX-toxin	1477:1485	arg1	antigens					1543:1550	serotype-specific antigens	1525:1550	serotype-specific antigens	1525:1550	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	11	42	from	resistance	2268:2277	arg1	pleuropneumoniae					2305:2320	A. pleuropneumoniae	2302:2320	A. pleuropneumoniae	2302:2320	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	0	43	theme	genomes	45:51	arg1	genomics					12:19	Comparative genomics	0:19	Comparative genomics of 26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae	0:112	Comparative genomics of 26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae.					
35196217	5	44	theme	de	985:986	arg1	assemblies					993:1002	PacBio- or ONT-only de novo assemblies	965:1002	PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads	965:1097	Genome assemblies were performed following two different strategies, i.e. PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads.					
35196217	7	45	theme	core	1263:1266	arg1	genes					1268:1272	core genes	1263:1272	core genes	1263:1272	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	10	46	theme	elements	1872:1879	arg1	presence					1847:1854	the presence	1843:1854	the presence of transposable elements surrounding these loci	1843:1902	Taken together with the presence of transposable elements surrounding these loci, we speculate a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer.					
35196217	8	47	theme	complete	1614:1621	arg1	I-F					1631:1633	complete subtype I-F	1614:1633	complete subtype I-F	1614:1633	We also identified small antibiotic resistance plasmids, and complete subtype I-F and subtype II-C CRISPR-Cas systems.					
35196217	1	48	theme	great	262:266	arg1	worldwide					284:292	great economic losses worldwide	262:292	great economic losses worldwide	262:292	Actinobacillus pleuropneumoniae is a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide.					
35196217	8	49	theme	I-F	1631:1633	arg1	systems					1663:1669	complete subtype I-F and subtype II-C CRISPR-Cas systems	1614:1669	complete subtype I-F and subtype II-C CRISPR-Cas systems	1614:1669	We also identified small antibiotic resistance plasmids, and complete subtype I-F and subtype II-C CRISPR-Cas systems.					
35196217	1	50	theme	losses	277:282	arg1	worldwide					284:292	great economic losses worldwide	262:292	great economic losses worldwide	262:292	Actinobacillus pleuropneumoniae is a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide.					
35196217	7	51	theme	core-genome	1232:1242	arg1	phylogeny					1244:1252	core-genome phylogeny	1232:1252	core-genome phylogeny based on core genes	1232:1272	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	8	52	theme	small	1572:1576	arg1	plasmids					1600:1607	small antibiotic resistance plasmids	1572:1607	small antibiotic resistance plasmids	1572:1607	We also identified small antibiotic resistance plasmids, and complete subtype I-F and subtype II-C CRISPR-Cas systems.					
35196217	8	53	theme	subtype	1639:1645	arg1	II-C					1647:1650	subtype II-C	1639:1650	subtype II-C	1639:1650	We also identified small antibiotic resistance plasmids, and complete subtype I-F and subtype II-C CRISPR-Cas systems.					
35196217	7	54	theme	phage	1442:1446	arg1	insertions					1448:1457	phage insertions	1442:1457	phage insertions	1442:1457	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	8	55	theme	resistance	1589:1598	arg1	plasmids					1600:1607	small antibiotic resistance plasmids	1572:1607	small antibiotic resistance plasmids	1572:1607	We also identified small antibiotic resistance plasmids, and complete subtype I-F and subtype II-C CRISPR-Cas systems.					
35196217	3	56	theme	serotypes	662:670	arg1	strains					638:644	the reference strains	624:644	the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform	624:777	We aimed to complete this picture by sequencing the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform.					
35196217	8	57	theme	CRISPR-Cas	1652:1661	arg1	systems					1663:1669	complete subtype I-F and subtype II-C CRISPR-Cas systems	1614:1669	complete subtype I-F and subtype II-C CRISPR-Cas systems	1614:1669	We also identified small antibiotic resistance plasmids, and complete subtype I-F and subtype II-C CRISPR-Cas systems.					
35196217	4	58	theme	serotypes	819:827	arg1	isolates					807:814	two field isolates	797:814	two field isolates of serotypes 2 and 3 that were PacBio- and MinION-sequenced, respectively	797:888	We also included two field isolates of serotypes 2 and 3 that were PacBio- and MinION-sequenced, respectively.					
35196217	11	59	theme	genes	2293:2297	arg1	evolution					2244:2252	the evolution	2240:2252	the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae	2240:2320	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	5	60	dep	assemblies	993:1002	arg1	i.e.					960:963	i.e.	960:963	i.e.	960:963	Genome assemblies were performed following two different strategies, i.e. PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads.					
35196217	10	61	theme	dynamic	1920:1926	arg1	exchange					1952:1959	a dynamic intra- and interspecies exchange	1918:1959	a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer	1918:2021	Taken together with the presence of transposable elements surrounding these loci, we speculate a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer.					
35196217	3	62	with	strains	638:644	arg1	sequencer					688:696	the MinION sequencer	677:696	the MinION sequencer (Oxford Nanopore Technologies, ONT)	677:732	We aimed to complete this picture by sequencing the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform.					
35196217	5	63	dep	de	985:986	arg1	novo					988:991	novo	988:991	novo	988:991	Genome assemblies were performed following two different strategies, i.e. PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads.					
35196217	1	64	theme	Pasteurellaceae	202:216	arg1	pleuropneumoniae					130:145	Actinobacillus pleuropneumoniae	115:145	Actinobacillus pleuropneumoniae	115:145	Actinobacillus pleuropneumoniae is a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide.					
35196217	1	64	theme	Pasteurellaceae	202:216	arg1	bacterium					178:186	a Gram-negative, rod-shaped bacterium	150:186	a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide	150:292	Actinobacillus pleuropneumoniae is a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide.					
35196217	1	65	theme	pig	226:228	arg1	pleuropneumonia					230:244	pig pleuropneumonia	226:244	pig pleuropneumonia associated with great economic losses worldwide	226:292	Actinobacillus pleuropneumoniae is a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide.					
35196217	2	66	theme	serotypes	551:559	arg1	strains					540:546	field strains	534:546	field strains of serotypes 1, 3, 7 and 8	534:573	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	7	67	theme	well-conserved	1364:1377	arg1	genomes					1379:1385	well-conserved genomes	1364:1385	well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS	1364:1466	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	11	68	theme	genomics	2057:2064	arg1	analysis					2066:2073	our comprehensive genomics analysis	2039:2073	our comprehensive genomics analysis	2039:2073	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	7	69	theme	genomes	1346:1352	arg1	cgMLST					1319:1324	cgMLST	1319:1324	cgMLST	1319:1324	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	7	69	theme	genomes	1346:1352	arg1	typing					1279:1284	SNP typing	1275:1284	SNP typing	1275:1284	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	7	69	theme	genomes	1346:1352	arg1	comparisons					1216:1226	blast-based genome comparisons	1197:1226	blast-based genome comparisons	1197:1226	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	7	69	theme	genomes	1346:1352	arg1	phylogeny					1244:1252	core-genome phylogeny	1232:1252	core-genome phylogeny based on core genes	1232:1272	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	7	69	theme	genomes	1346:1352	arg1	typing					1311:1316	multi-locus sequence typing	1290:1316	multi-locus sequence typing (cgMLST)	1290:1325	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	10	70	theme	factors	1987:1993	arg1	exchange					1952:1959	a dynamic intra- and interspecies exchange	1918:1959	a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer	1918:2021	Taken together with the presence of transposable elements surrounding these loci, we speculate a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer.					
35196217	9	71	theme	similar	1688:1694	arg1	clusters					1696:1703	highly similar clusters	1681:1703	highly similar clusters encoding all those serotype-specific traits	1681:1747	Of note, highly similar clusters encoding all those serotype-specific traits were also found in other pathogenic and commensal Actinobacillus species.					
35196217	6	72	theme	comparable	1175:1184	arg1	qualities					1186:1194	comparable qualities	1175:1194	comparable qualities	1175:1194	Both methods proved successful in obtaining accurate circular genomes with comparable qualities.					
35196217	10	73	theme	such	1964:1967	arg1	factors					1987:1993	such virulence-related factors	1964:1993	such virulence-related factors	1964:1993	Taken together with the presence of transposable elements surrounding these loci, we speculate a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer.					
35196217	3	74	theme	Illumina	760:767	arg1	platform					770:777	an Illumina HiSeq (Illumina) platform	741:777	an Illumina HiSeq (Illumina) platform	741:777	We aimed to complete this picture by sequencing the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform.					
35196217	0	75	theme	different	59:67	arg1	serotypes					69:77	18 different serotypes	56:77	18 different serotypes of Actinobacillus pleuropneumoniae	56:112	Comparative genomics of 26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae.					
35196217	11	76	from	genes	2293:2297	arg1	pleuropneumoniae					2305:2320	A. pleuropneumoniae	2302:2320	A. pleuropneumoniae	2302:2320	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	7	77	theme	serotype-specific	1525:1541	arg1	antigens					1543:1550	serotype-specific antigens	1525:1550	serotype-specific antigens	1525:1550	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	7	77	theme	serotype-specific	1525:1541	arg1	clusters					1487:1494	RTX-toxin clusters	1477:1494	RTX-toxin clusters	1477:1494	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	6	78	theme	accurate	1144:1151	arg1	genomes					1162:1168	accurate circular genomes	1144:1168	accurate circular genomes	1144:1168	Both methods proved successful in obtaining accurate circular genomes with comparable qualities.					
35196217	10	79	theme	horizontal	1998:2007	arg1	transfer					2014:2021	horizontal gene transfer	1998:2021	horizontal gene transfer	1998:2021	Taken together with the presence of transposable elements surrounding these loci, we speculate a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer.					
35196217	10	80	theme	interspecies	1939:1950	arg1	exchange					1952:1959	a dynamic intra- and interspecies exchange	1918:1959	a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer	1918:2021	Taken together with the presence of transposable elements surrounding these loci, we speculate a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer.					
35196217	10	81	theme	transposable	1859:1870	arg1	elements					1872:1879	transposable elements	1859:1879	transposable elements surrounding these loci	1859:1902	Taken together with the presence of transposable elements surrounding these loci, we speculate a dynamic intra- and interspecies exchange of such virulence-related factors by horizontal gene transfer.					
35196217	0	82	theme	pleuropneumoniae	97:112	arg1	serotypes					69:77	18 different serotypes	56:77	18 different serotypes of Actinobacillus pleuropneumoniae	56:112	Comparative genomics of 26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae.					
35196217	11	83	theme	resistance	2268:2277	arg1	evolution					2244:2252	the evolution	2240:2252	the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae	2240:2320	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	1	84	dep	Gram-negative	152:164	arg1	rod-shaped					167:176	rod-shaped	167:176	rod-shaped	167:176	Actinobacillus pleuropneumoniae is a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide.					
35196217	7	85	theme	sequence	1302:1309	arg1	cgMLST					1319:1324	cgMLST	1319:1324	cgMLST	1319:1324	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	7	85	theme	sequence	1302:1309	arg1	typing					1311:1316	multi-locus sequence typing	1290:1316	multi-locus sequence typing (cgMLST)	1290:1325	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	2	86	theme	complete	420:427	arg1	genomes					438:444	complete circular genomes	420:444	complete circular genomes	420:444	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	9	87	theme	pathogenic	1774:1783	arg1	species					1814:1820	other pathogenic and commensal Actinobacillus species	1768:1820	other pathogenic and commensal Actinobacillus species	1768:1820	Of note, highly similar clusters encoding all those serotype-specific traits were also found in other pathogenic and commensal Actinobacillus species.					
35196217	5	88	theme	Genome	891:896	arg1	assemblies					898:907	Genome assemblies	891:907	Genome assemblies	891:907	Genome assemblies were performed following two different strategies, i.e. PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads.					
35196217	7	89	theme	SNP	1275:1277	arg1	typing					1279:1284	SNP typing	1275:1284	SNP typing	1275:1284	blast-based genome comparisons and core-genome phylogeny based on core genes, SNP typing and multi-locus sequence typing (cgMLST) of the 26 circular genomes indicated well-conserved genomes across the 18 different serotypes, differing mainly in phage insertions, and CPS, LPS and RTX-toxin clusters, which, consistently, encode serotype-specific antigens.					
35196217	9	90	theme	commensal	1789:1797	arg1	species					1814:1820	other pathogenic and commensal Actinobacillus species	1768:1820	other pathogenic and commensal Actinobacillus species	1768:1820	Of note, highly similar clusters encoding all those serotype-specific traits were also found in other pathogenic and commensal Actinobacillus species.					
35196217	5	91	dep	combining	1066:1074	arg1	reads					1093:1097	reads	1093:1097	combining ONT and Illumina reads	1066:1097	Genome assemblies were performed following two different strategies, i.e. PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads.					
35196217	11	92	from	evolution	2244:2252	arg1	pleuropneumoniae					2305:2320	A. pleuropneumoniae	2302:2320	A. pleuropneumoniae	2302:2320	In conclusion, our comprehensive genomics analysis provides useful information for diagnostic test and vaccine development, but also for whole-genome-based epidemiological studies, as well as for the surveillance of the evolution of antibiotic resistance and virulence genes in A. pleuropneumoniae.					
35196217	0	93	theme	circular	36:43	arg1	genomes					45:51	26 complete circular genomes	24:51	26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae	24:112	Comparative genomics of 26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae.					
35196217	5	94	theme	PacBio-	965:971	arg1	assemblies					993:1002	PacBio- or ONT-only de novo assemblies	965:1002	PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads	965:1097	Genome assemblies were performed following two different strategies, i.e. PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads.					
35196217	2	95	theme	serotypes	503:511	arg1	5b					522:523	5b	522:523	5b	522:523	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	2	95	theme	serotypes	503:511	arg1	strains					492:498	the reference strains	478:498	the reference strains of serotypes 1	478:513	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	5	96	dep	Illumina	1018:1025	arg1	reads					1027:1031	reads	1027:1031	reads	1027:1031	Genome assemblies were performed following two different strategies, i.e. PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads.					
35196217	3	97	dep	sequencer	688:696	arg1	ONT					729:731	ONT	729:731	ONT	729:731	We aimed to complete this picture by sequencing the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform.					
35196217	3	97	dep	sequencer	688:696	arg1	Technologies					715:726	Oxford Nanopore Technologies	699:726	Oxford Nanopore Technologies	699:726	We aimed to complete this picture by sequencing the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform.					
35196217	5	98	theme	ONT-only	976:983	arg1	assemblies					993:1002	PacBio- or ONT-only de novo assemblies	965:1002	PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads	965:1097	Genome assemblies were performed following two different strategies, i.e. PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads.					
35196217	8	99	theme	subtype	1623:1629	arg1	I-F					1631:1633	complete subtype I-F	1614:1633	complete subtype I-F	1614:1633	We also identified small antibiotic resistance plasmids, and complete subtype I-F and subtype II-C CRISPR-Cas systems.					
35196217	5	100	theme	different	938:946	arg1	strategies					948:957	two different strategies	934:957	two different strategies	934:957	Genome assemblies were performed following two different strategies, i.e. PacBio- or ONT-only de novo assemblies polished with Illumina reads or a hybrid assembly by directly combining ONT and Illumina reads.					
35196217	3	101	theme	reference	628:636	arg1	strains					638:644	the reference strains	624:644	the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform	624:777	We aimed to complete this picture by sequencing the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform.					
35196217	2	102	theme	CPS	370:372	arg1	compositions					375:386	capsular (CPS) compositions	360:386	capsular (CPS) compositions	360:386	Nineteen serotypes with distinctive lipopolysaccharide (LPS) and capsular (CPS) compositions have been described so far, yet complete circular genomes are publicly available only for the reference strains of serotypes 1, 4 and 5b, and for field strains of serotypes 1, 3, 7 and 8.					
35196217	1	103	theme	economic	268:275	arg1	worldwide					284:292	great economic losses worldwide	262:292	great economic losses worldwide	262:292	Actinobacillus pleuropneumoniae is a Gram-negative, rod-shaped bacterium of the family Pasteurellaceae causing pig pleuropneumonia associated with great economic losses worldwide.					
35196217	8	104	theme	antibiotic	1578:1587	arg1	plasmids					1600:1607	small antibiotic resistance plasmids	1572:1607	small antibiotic resistance plasmids	1572:1607	We also identified small antibiotic resistance plasmids, and complete subtype I-F and subtype II-C CRISPR-Cas systems.					
35196217	3	105	from	strains	638:644	arg1	platform					770:777	an Illumina HiSeq (Illumina) platform	741:777	an Illumina HiSeq (Illumina) platform	741:777	We aimed to complete this picture by sequencing the reference strains of 17 different serotypes with the MinION sequencer (Oxford Nanopore Technologies, ONT) and on an Illumina HiSeq (Illumina) platform.					
35196217	9	106	theme	other	1768:1772	arg1	species					1814:1820	other pathogenic and commensal Actinobacillus species	1768:1820	other pathogenic and commensal Actinobacillus species	1768:1820	Of note, highly similar clusters encoding all those serotype-specific traits were also found in other pathogenic and commensal Actinobacillus species.					
35196217	8	107	theme	II-C	1647:1650	arg1	systems					1663:1669	complete subtype I-F and subtype II-C CRISPR-Cas systems	1614:1669	complete subtype I-F and subtype II-C CRISPR-Cas systems	1614:1669	We also identified small antibiotic resistance plasmids, and complete subtype I-F and subtype II-C CRISPR-Cas systems.					
37276906	4	0	theme	deproteinization	1009:1024	arg1	model					1026:1030	the deproteinization model	1005:1030	the deproteinization model	1005:1030	According to the optimization, which was performed using a response surface methodology based on a central composite design, the demineralization model is more complicated than the deproteinization model.					
37276906	1	1	theme	Persian	337:343	arg1	zone					350:353	the Persian Gulf zone	333:353	the Persian Gulf zone	333:353	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	1	2	from	waste	324:328	arg1	zone					350:353	the Persian Gulf zone	333:353	the Persian Gulf zone	333:353	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	1	2	from	waste	324:328	arg1	extraction					289:298	biological chitin extraction	271:298	biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone	271:353	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	9	3	theme	380 °C	1550:1555	arg1	temperature					1535:1545	a maximum degradation temperature	1513:1545	a maximum degradation temperature of 380 °C	1513:1555	Besides, the thermal stability of the extracted chitin, with a maximum degradation temperature of 380 °C is comparable with the literature data.					
37276906	1	4	theme	operating	246:254	arg1	conditions					256:265	the operating conditions	242:265	the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone	242:353	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	4	5	theme	composite	935:943	arg1	design					945:950	a central composite design	925:950	a central composite design	925:950	According to the optimization, which was performed using a response surface methodology based on a central composite design, the demineralization model is more complicated than the deproteinization model.					
37276906	1	6	theme	Gulf	345:348	arg1	zone					350:353	the Persian Gulf zone	333:353	the Persian Gulf zone	333:353	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	4	7	theme	response	887:894	arg1	methodology					904:914	a response surface methodology	885:914	a response surface methodology based on a central composite design	885:950	According to the optimization, which was performed using a response surface methodology based on a central composite design, the demineralization model is more complicated than the deproteinization model.					
37276906	3	8	theme	ready-to-use	703:714	arg1	medium					727:732	the purchased and ready-to-use commercial medium	685:732	the purchased and ready-to-use commercial medium	685:732	It was found that the proposed non-commercial culture medium was more efficient than the purchased and ready-to-use commercial medium and increased deproteinization and demineralization efficiency by 9 % and 11 %, respectively.					
37276906	6	9	theme	chitin	1270:1275	arg1	acetylation					1241:1251	acetylation	1241:1251	acetylation of the extracted chitin	1241:1275	The FTIR results revealed that shrimp shells and chitin have similar main functional groups, while the degree of acetylation of the extracted chitin was 62.26 %.					
37276906	8	10	theme	XRD	1385:1387	arg1	data					1389:1392	The XRD data	1381:1392	The XRD data	1381:1392	The XRD data showed that the crystallinity index of chitin was 93.9 %.					
37276906	8	11	theme	crystallinity	1410:1422	arg1	%					1449:1449	93.9 %	1444:1449	93.9 %	1444:1449	The XRD data showed that the crystallinity index of chitin was 93.9 %.					
37276906	8	11	theme	crystallinity	1410:1422	arg1	index					1424:1428	the crystallinity index	1406:1428	the crystallinity index of chitin	1406:1438	The XRD data showed that the crystallinity index of chitin was 93.9 %.					
37276906	0	12	theme	comparative	82:92	arg1	study					94:98	A comparative study	80:98	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.	0:153	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.					
37276906	3	13	theme	deproteinization	748:763	arg1	efficiency					786:795	deproteinization and demineralization efficiency	748:795	deproteinization and demineralization efficiency	748:795	It was found that the proposed non-commercial culture medium was more efficient than the purchased and ready-to-use commercial medium and increased deproteinization and demineralization efficiency by 9 % and 11 %, respectively.					
37276906	4	14	theme	surface	896:902	arg1	methodology					904:914	a response surface methodology	885:914	a response surface methodology based on a central composite design	885:950	According to the optimization, which was performed using a response surface methodology based on a central composite design, the demineralization model is more complicated than the deproteinization model.					
37276906	1	15	theme	biological	271:280	arg1	extraction					289:298	biological chitin extraction	271:298	biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone	271:353	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	0	16	theme	culture	103:109	arg1	mediums					111:117	culture mediums	103:117	culture mediums	103:117	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.					
37276906	9	17	theme	extracted	1490:1498	arg1	chitin					1500:1505	the extracted chitin	1486:1505	the extracted chitin	1486:1505	Besides, the thermal stability of the extracted chitin, with a maximum degradation temperature of 380 °C is comparable with the literature data.					
37276906	9	18	with	comparable	1560:1569	arg1	data					1591:1594	the literature data	1576:1594	the literature data	1576:1594	Besides, the thermal stability of the extracted chitin, with a maximum degradation temperature of 380 °C is comparable with the literature data.					
37276906	6	19	theme	FTIR	1132:1135	arg1	results					1137:1143	The FTIR results	1128:1143	The FTIR results	1128:1143	The FTIR results revealed that shrimp shells and chitin have similar main functional groups, while the degree of acetylation of the extracted chitin was 62.26 %.					
37276906	9	20	theme	literature	1580:1589	arg1	data					1591:1594	the literature data	1576:1594	the literature data	1576:1594	Besides, the thermal stability of the extracted chitin, with a maximum degradation temperature of 380 °C is comparable with the literature data.					
37276906	2	21	theme	shrimp	520:525	arg1	shells					527:532	the shrimp shells	516:532	the shrimp shells	516:532	For this purpose, the two most commonly used microorganisms, Bacillus licheniformis and Lactobacillus plantarum, were obtained to deproteinize and demineralize the shrimp shells within both culture mediums using a successive two-stage process.					
37276906	0	22	theme	chitin	10:15	arg1	extraction					17:26	Microbial chitin extraction	0:26	Microbial chitin extraction	0:26	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.					
37276906	7	23	theme	microfibrils	1331:1342	arg1	porosity					1371:1378	the chitin structure's porosity	1348:1378	the chitin structure's porosity	1348:1378	SEM results illustrated the formation of microfibrils and the chitin structure's porosity.					
37276906	7	23	theme	microfibrils	1331:1342	arg1	formation					1318:1326	the formation	1314:1326	the formation of microfibrils	1314:1342	SEM results illustrated the formation of microfibrils and the chitin structure's porosity.					
37276906	9	24	theme	chitin	1500:1505	arg1	stability					1473:1481	the thermal stability	1461:1481	the thermal stability	1461:1481	Besides, the thermal stability of the extracted chitin, with a maximum degradation temperature of 380 °C is comparable with the literature data.					
37276906	9	24	theme	chitin	1500:1505	arg1	comparable					1560:1569	comparable	1560:1569	comparable	1560:1569	Besides, the thermal stability of the extracted chitin, with a maximum degradation temperature of 380 °C is comparable with the literature data.					
37276906	0	25	theme	Microbial	0:8	arg1	extraction					17:26	Microbial chitin extraction	0:26	Microbial chitin extraction	0:26	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.					
37276906	1	26	theme	chitin	282:287	arg1	extraction					289:298	biological chitin extraction	271:298	biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone	271:353	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	6	27	theme	functional	1202:1211	arg1	groups					1213:1218	similar main functional groups	1189:1218	similar main functional groups	1189:1218	The FTIR results revealed that shrimp shells and chitin have similar main functional groups, while the degree of acetylation of the extracted chitin was 62.26 %.					
37276906	2	28	theme	culture	546:552	arg1	mediums					554:560	both culture mediums	541:560	both culture mediums using a successive two-stage process	541:597	For this purpose, the two most commonly used microorganisms, Bacillus licheniformis and Lactobacillus plantarum, were obtained to deproteinize and demineralize the shrimp shells within both culture mediums using a successive two-stage process.					
37276906	6	29	theme	acetylation	1241:1251	arg1	%					1287:1287	62.26 %	1281:1287	62.26 %	1281:1287	The FTIR results revealed that shrimp shells and chitin have similar main functional groups, while the degree of acetylation of the extracted chitin was 62.26 %.					
37276906	6	29	theme	acetylation	1241:1251	arg1	degree					1231:1236	the degree	1227:1236	the degree of acetylation of the extracted chitin	1227:1275	The FTIR results revealed that shrimp shells and chitin have similar main functional groups, while the degree of acetylation of the extracted chitin was 62.26 %.					
37276906	6	30	theme	main	1197:1200	arg1	groups					1213:1218	similar main functional groups	1189:1218	similar main functional groups	1189:1218	The FTIR results revealed that shrimp shells and chitin have similar main functional groups, while the degree of acetylation of the extracted chitin was 62.26 %.					
37276906	1	31	theme	low-cost	189:196	arg1	medium					222:227	a low-cost, non-commercial culture medium	187:227	a low-cost, non-commercial culture medium	187:227	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	3	32	theme	demineralization	769:784	arg1	efficiency					786:795	deproteinization and demineralization efficiency	748:795	deproteinization and demineralization efficiency	748:795	It was found that the proposed non-commercial culture medium was more efficient than the purchased and ready-to-use commercial medium and increased deproteinization and demineralization efficiency by 9 % and 11 %, respectively.					
37276906	0	33	theme	mediums	111:117	arg1	study					94:98	A comparative study	80:98	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.	0:153	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.					
37276906	6	34	theme	similar	1189:1195	arg1	groups					1213:1218	similar main functional groups	1189:1218	similar main functional groups	1189:1218	The FTIR results revealed that shrimp shells and chitin have similar main functional groups, while the degree of acetylation of the extracted chitin was 62.26 %.					
37276906	7	35	theme	chitin	1352:1357	arg1	structure					1359:1367	the chitin structure	1348:1367	the chitin structure's porosity	1348:1378	SEM results illustrated the formation of microfibrils and the chitin structure's porosity.					
37276906	1	36	theme	green	305:309	arg1	waste					324:328	green tiger shrimp waste	305:328	green tiger shrimp waste in the Persian Gulf zone	305:353	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	0	37	theme	bioprocess	130:139	arg1	optimization					141:152	bioprocess optimization	130:152	bioprocess optimization	130:152	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.					
37276906	7	38	theme	SEM	1290:1292	arg1	results					1294:1300	SEM results	1290:1300	SEM results	1290:1300	SEM results illustrated the formation of microfibrils and the chitin structure's porosity.					
37276906	8	39	theme	chitin	1433:1438	arg1	%					1449:1449	93.9 %	1444:1449	93.9 %	1444:1449	The XRD data showed that the crystallinity index of chitin was 93.9 %.					
37276906	8	39	theme	chitin	1433:1438	arg1	index					1424:1428	the crystallinity index	1406:1428	the crystallinity index of chitin	1406:1438	The XRD data showed that the crystallinity index of chitin was 93.9 %.					
37276906	2	40	theme	used	396:399	arg1	microorganisms					401:414	the two most commonly used microorganisms	374:414	the two most commonly used microorganisms	374:414	For this purpose, the two most commonly used microorganisms, Bacillus licheniformis and Lactobacillus plantarum, were obtained to deproteinize and demineralize the shrimp shells within both culture mediums using a successive two-stage process.					
37276906	2	40	theme	used	396:399	arg1	licheniformis					426:438	Bacillus licheniformis	417:438	Bacillus licheniformis	417:438	For this purpose, the two most commonly used microorganisms, Bacillus licheniformis and Lactobacillus plantarum, were obtained to deproteinize and demineralize the shrimp shells within both culture mediums using a successive two-stage process.					
37276906	2	40	theme	used	396:399	arg1	plantarum					458:466	Lactobacillus plantarum	444:466	Lactobacillus plantarum	444:466	For this purpose, the two most commonly used microorganisms, Bacillus licheniformis and Lactobacillus plantarum, were obtained to deproteinize and demineralize the shrimp shells within both culture mediums using a successive two-stage process.					
37276906	1	41	theme	tiger	311:315	arg1	waste					324:328	green tiger shrimp waste	305:328	green tiger shrimp waste in the Persian Gulf zone	305:353	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	3	42	theme	non-commercial	631:644	arg1	medium					654:659	the proposed non-commercial culture medium	618:659	the proposed non-commercial culture medium	618:659	It was found that the proposed non-commercial culture medium was more efficient than the purchased and ready-to-use commercial medium and increased deproteinization and demineralization efficiency by 9 % and 11 %, respectively.					
37276906	3	42	theme	non-commercial	631:644	arg1	efficient					670:678	efficient	670:678	efficient	670:678	It was found that the proposed non-commercial culture medium was more efficient than the purchased and ready-to-use commercial medium and increased deproteinization and demineralization efficiency by 9 % and 11 %, respectively.					
37276906	0	43	from	waste	73:77	arg1	extraction					17:26	Microbial chitin extraction	0:26	Microbial chitin extraction	0:26	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.					
37276906	0	43	from	waste	73:77	arg1	characterization					32:47	characterization	32:47	characterization	32:47	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.					
37276906	1	44	theme	culture	214:220	arg1	medium					222:227	a low-cost, non-commercial culture medium	187:227	a low-cost, non-commercial culture medium	187:227	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	0	45	theme	tiger	60:64	arg1	waste					73:77	green tiger shrimp waste	54:77	green tiger shrimp waste	54:77	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.					
37276906	1	46	theme	shrimp	317:322	arg1	waste					324:328	green tiger shrimp waste	305:328	green tiger shrimp waste in the Persian Gulf zone	305:353	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	9	47	with	stability	1473:1481	arg1	temperature					1535:1545	a maximum degradation temperature	1513:1545	a maximum degradation temperature of 380 °C	1513:1555	Besides, the thermal stability of the extracted chitin, with a maximum degradation temperature of 380 °C is comparable with the literature data.					
37276906	6	48	contain	have	1184:1187	arg1	chitin					1177:1182	chitin	1177:1182	chitin	1177:1182	The FTIR results revealed that shrimp shells and chitin have similar main functional groups, while the degree of acetylation of the extracted chitin was 62.26 %.					
37276906	6	48	contain	have	1184:1187	arg2	groups					1213:1218	similar main functional groups	1189:1218	similar main functional groups	1189:1218	The FTIR results revealed that shrimp shells and chitin have similar main functional groups, while the degree of acetylation of the extracted chitin was 62.26 %.					
37276906	6	48	contain	have	1184:1187	arg1	shells					1166:1171	shrimp shells	1159:1171	shrimp shells	1159:1171	The FTIR results revealed that shrimp shells and chitin have similar main functional groups, while the degree of acetylation of the extracted chitin was 62.26 %.					
37276906	5	49	theme	deproteinization	1063:1078	arg1	yields					1101:1106	deproteinization and demineralization yields	1063:1106	deproteinization and demineralization yields	1063:1106	The presented model predicted deproteinization and demineralization yields with good accuracy.					
37276906	4	50	theme	central	927:933	arg1	design					945:950	a central composite design	925:950	a central composite design	925:950	According to the optimization, which was performed using a response surface methodology based on a central composite design, the demineralization model is more complicated than the deproteinization model.					
37276906	0	51	theme	green	54:58	arg1	waste					73:77	green tiger shrimp waste	54:77	green tiger shrimp waste	54:77	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.					
37276906	2	52	theme	two-stage	581:589	arg1	process					591:597	a successive two-stage process	568:597	a successive two-stage process	568:597	For this purpose, the two most commonly used microorganisms, Bacillus licheniformis and Lactobacillus plantarum, were obtained to deproteinize and demineralize the shrimp shells within both culture mediums using a successive two-stage process.					
37276906	5	53	theme	demineralization	1084:1099	arg1	yields					1101:1106	deproteinization and demineralization yields	1063:1106	deproteinization and demineralization yields	1063:1106	The presented model predicted deproteinization and demineralization yields with good accuracy.					
37276906	6	54	theme	extracted	1260:1268	arg1	chitin					1270:1275	the extracted chitin	1256:1275	the extracted chitin	1256:1275	The FTIR results revealed that shrimp shells and chitin have similar main functional groups, while the degree of acetylation of the extracted chitin was 62.26 %.					
37276906	3	55	theme	culture	646:652	arg1	medium					654:659	the proposed non-commercial culture medium	618:659	the proposed non-commercial culture medium	618:659	It was found that the proposed non-commercial culture medium was more efficient than the purchased and ready-to-use commercial medium and increased deproteinization and demineralization efficiency by 9 % and 11 %, respectively.					
37276906	3	55	theme	culture	646:652	arg1	efficient					670:678	efficient	670:678	efficient	670:678	It was found that the proposed non-commercial culture medium was more efficient than the purchased and ready-to-use commercial medium and increased deproteinization and demineralization efficiency by 9 % and 11 %, respectively.					
37276906	2	56	theme	successive	570:579	arg1	process					591:597	a successive two-stage process	568:597	a successive two-stage process	568:597	For this purpose, the two most commonly used microorganisms, Bacillus licheniformis and Lactobacillus plantarum, were obtained to deproteinize and demineralize the shrimp shells within both culture mediums using a successive two-stage process.					
37276906	3	57	theme	purchased	689:697	arg1	medium					727:732	the purchased and ready-to-use commercial medium	685:732	the purchased and ready-to-use commercial medium	685:732	It was found that the proposed non-commercial culture medium was more efficient than the purchased and ready-to-use commercial medium and increased deproteinization and demineralization efficiency by 9 % and 11 %, respectively.					
37276906	3	58	theme	proposed	622:629	arg1	medium					654:659	the proposed non-commercial culture medium	618:659	the proposed non-commercial culture medium	618:659	It was found that the proposed non-commercial culture medium was more efficient than the purchased and ready-to-use commercial medium and increased deproteinization and demineralization efficiency by 9 % and 11 %, respectively.					
37276906	3	58	theme	proposed	622:629	arg1	efficient					670:678	efficient	670:678	efficient	670:678	It was found that the proposed non-commercial culture medium was more efficient than the purchased and ready-to-use commercial medium and increased deproteinization and demineralization efficiency by 9 % and 11 %, respectively.					
37276906	9	59	theme	maximum	1515:1521	arg1	temperature					1535:1545	a maximum degradation temperature	1513:1545	a maximum degradation temperature of 380 °C	1513:1555	Besides, the thermal stability of the extracted chitin, with a maximum degradation temperature of 380 °C is comparable with the literature data.					
37276906	0	60	dep	extraction	17:26	arg1	study					94:98	A comparative study	80:98	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.	0:153	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.					
37276906	9	61	theme	degradation	1523:1533	arg1	temperature					1535:1545	a maximum degradation temperature	1513:1545	a maximum degradation temperature of 380 °C	1513:1555	Besides, the thermal stability of the extracted chitin, with a maximum degradation temperature of 380 °C is comparable with the literature data.					
37276906	5	62	theme	good	1113:1116	arg1	accuracy					1118:1125	good accuracy	1113:1125	good accuracy	1113:1125	The presented model predicted deproteinization and demineralization yields with good accuracy.					
37276906	1	63	dep	low-cost	189:196	arg1	non-commercial					199:212	non-commercial	199:212	non-commercial	199:212	This research aims to introduce a low-cost, non-commercial culture medium and optimize the operating conditions for biological chitin extraction from green tiger shrimp waste in the Persian Gulf zone.					
37276906	6	64	theme	shrimp	1159:1164	arg1	shells					1166:1171	shrimp shells	1159:1171	shrimp shells	1159:1171	The FTIR results revealed that shrimp shells and chitin have similar main functional groups, while the degree of acetylation of the extracted chitin was 62.26 %.					
37276906	9	65	theme	thermal	1465:1471	arg1	stability					1473:1481	the thermal stability	1461:1481	the thermal stability	1461:1481	Besides, the thermal stability of the extracted chitin, with a maximum degradation temperature of 380 °C is comparable with the literature data.					
37276906	9	65	theme	thermal	1465:1471	arg1	comparable					1560:1569	comparable	1560:1569	comparable	1560:1569	Besides, the thermal stability of the extracted chitin, with a maximum degradation temperature of 380 °C is comparable with the literature data.					
37276906	0	66	theme	shrimp	66:71	arg1	waste					73:77	green tiger shrimp waste	54:77	green tiger shrimp waste	54:77	Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization.					
37276906	3	67	theme	commercial	716:725	arg1	medium					727:732	the purchased and ready-to-use commercial medium	685:732	the purchased and ready-to-use commercial medium	685:732	It was found that the proposed non-commercial culture medium was more efficient than the purchased and ready-to-use commercial medium and increased deproteinization and demineralization efficiency by 9 % and 11 %, respectively.					
37276906	5	68	theme	presented	1037:1045	arg1	model					1047:1051	The presented model	1033:1051	The presented model	1033:1051	The presented model predicted deproteinization and demineralization yields with good accuracy.					
37276906	4	69	theme	demineralization	957:972	arg1	model					974:978	the demineralization model	953:978	the demineralization model	953:978	According to the optimization, which was performed using a response surface methodology based on a central composite design, the demineralization model is more complicated than the deproteinization model.					
37276906	4	69	theme	demineralization	957:972	arg1	complicated					988:998	complicated	988:998	complicated	988:998	According to the optimization, which was performed using a response surface methodology based on a central composite design, the demineralization model is more complicated than the deproteinization model.					
36867861	0	0	theme	Regenerated	68:78	arg1	Fibers					90:95	Regenerated Cellulose Fibers	68:95	Regenerated Cellulose Fibers	68:95	Effects of Glucose and Coagulant on the Structure and Properties of Regenerated Cellulose Fibers.					
36867861	5	1	theme	bath	1071:1074	arg1	composition					1076:1086	coagulation bath composition	1059:1086	coagulation bath composition	1059:1086	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	4	2	theme	pulp	852:855	arg1	WPC					868:870	WPC	868:870	WPC	868:870	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	4	2	theme	pulp	852:855	arg1	cellulose					857:865	wood pulp cellulose	847:865	wood pulp cellulose (WPC)	847:871	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	4	3	with	phosphate	768:776	arg1	contents					813:820	different glucose contents	795:820	different glucose contents	795:820	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	6	4	theme	glucose	1334:1340	arg1	presence					1322:1329	the presence	1318:1329	the presence of glucose in the spinning solution or coagulation bath	1318:1385	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	5	5	theme	composition	1076:1086	arg1	influence					1046:1054	the influence	1042:1054	the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs	1042:1181	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	1	6	theme	environmentally	138:152	arg1	material					163:170	an environmentally friendly material	135:170	an environmentally friendly material	135:170	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	1	6	theme	environmentally	138:152	arg1	fiber					120:124	Regenerated cellulose fiber	98:124	Regenerated cellulose fiber (RCF)	98:130	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	5	7	dep	characteristics	1129:1143	arg1	the					1111:1113	the	1111:1113	the	1111:1113	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	6	8	theme	coagulation	1370:1380	arg1	bath					1382:1385	coagulation bath	1370:1385	coagulation bath	1370:1385	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	0	9	theme	Fibers	90:95	arg1	Properties					54:63	Properties	54:63	Properties	54:63	Effects of Glucose and Coagulant on the Structure and Properties of Regenerated Cellulose Fibers.					
36867861	0	9	theme	Fibers	90:95	arg1	Structure					40:48	Structure	40:48	Structure	40:48	Effects of Glucose and Coagulant on the Structure and Properties of Regenerated Cellulose Fibers.					
36867861	0	10	from	Effects	0:6	arg1	Properties					54:63	Properties	54:63	Properties	54:63	Effects of Glucose and Coagulant on the Structure and Properties of Regenerated Cellulose Fibers.					
36867861	0	10	from	Effects	0:6	arg1	Structure					40:48	Structure	40:48	Structure	40:48	Effects of Glucose and Coagulant on the Structure and Properties of Regenerated Cellulose Fibers.					
36867861	6	11	theme	industrial	1508:1517	arg1	production					1519:1528	the industrial production	1504:1528	the industrial production of new type fiber	1504:1546	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	4	12	from	RCFs	886:889	arg1	baths					916:920	different coagulation baths	894:920	different coagulation baths	894:920	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	0	13	theme	Cellulose	80:88	arg1	Fibers					90:95	Regenerated Cellulose Fibers	68:95	Regenerated Cellulose Fibers	68:95	Effects of Glucose and Coagulant on the Structure and Properties of Regenerated Cellulose Fibers.					
36867861	1	14	theme	large	255:259	arg1	number					261:266	a large number	253:266	a large number of applications	253:282	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	4	15	theme	different	894:902	arg1	baths					916:920	different coagulation baths	894:920	different coagulation baths	894:920	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	2	16	theme	ionic	328:332	arg1	ILs					343:345	ILs	343:345	ILs	343:345	However, during the spinning process using ionic liquids (ILs) as solvents, the dissolved cellulose continues to degrade and even produces degradation products such as glucose, which can enter the recycled solvent and coagulation bath.					
36867861	2	16	theme	ionic	328:332	arg1	solvents					351:358	solvents	351:358	solvents	351:358	However, during the spinning process using ionic liquids (ILs) as solvents, the dissolved cellulose continues to degrade and even produces degradation products such as glucose, which can enter the recycled solvent and coagulation bath.					
36867861	2	16	theme	ionic	328:332	arg1	liquids					334:340	ionic liquids	328:340	ionic liquids (ILs)	328:346	However, during the spinning process using ionic liquids (ILs) as solvents, the dissolved cellulose continues to degrade and even produces degradation products such as glucose, which can enter the recycled solvent and coagulation bath.					
36867861	6	17	from	presence	1322:1329	arg1	solution					1358:1365	the spinning solution	1345:1365	the spinning solution	1345:1365	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	6	17	from	presence	1322:1329	arg1	bath					1382:1385	coagulation bath	1370:1385	coagulation bath	1370:1385	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	5	18	from	content	945:951	arg1	solution					965:972	spinning solution	956:972	spinning solution	956:972	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	1	19	theme	friendly	154:161	arg1	material					163:170	an environmentally friendly material	135:170	an environmentally friendly material	135:170	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	1	19	theme	friendly	154:161	arg1	fiber					120:124	Regenerated cellulose fiber	98:124	Regenerated cellulose fiber (RCF)	98:130	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	5	20	theme	RCFs	1178:1181	arg1	characteristics					1129:1143	morphological characteristics	1115:1143	morphological characteristics	1115:1143	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	5	20	theme	RCFs	1178:1181	arg1	properties					1160:1169	mechanical properties	1149:1169	mechanical properties	1149:1169	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	6	21	from	changes	1415:1421	arg1	properties					1437:1446	mechanical properties	1426:1446	mechanical properties	1426:1446	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	5	22	from	effect	927:932	arg1	solution					965:972	spinning solution	956:972	spinning solution	956:972	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	5	22	from	effect	927:932	arg1	spinnability					983:994	fiber spinnability	977:994	fiber spinnability	977:994	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	6	23	dep	morphology	1241:1250	arg1	the					1237:1239	the	1237:1239	the	1237:1239	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	5	24	from	influence	1046:1054	arg1	characteristics					1129:1143	morphological characteristics	1115:1143	morphological characteristics	1115:1143	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	5	24	from	influence	1046:1054	arg1	properties					1160:1169	mechanical properties	1149:1169	mechanical properties	1149:1169	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	4	25	theme	glucose	805:811	arg1	contents					813:820	different glucose contents	795:820	different glucose contents	795:820	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	6	26	theme	corresponding	1401:1413	arg1	changes					1415:1421	corresponding changes	1401:1421	corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber	1401:1546	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	3	27	dep	affect	559:564	arg1	so					634:635	so	634:635	so	634:635	The presence of glucose can seriously affect the performance of the produced RCFs and hinder their applications, so it has become critical to clarify the regulation and mechanism of this process.					
36867861	5	28	theme	morphological	1115:1127	arg1	characteristics					1129:1143	morphological characteristics	1115:1143	morphological characteristics	1115:1143	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	4	29	theme	different	795:803	arg1	contents					813:820	different glucose contents	795:820	different glucose contents	795:820	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	6	30	attach	presence	1322:1329	arg1	solution					1358:1365	the spinning solution	1345:1365	the spinning solution	1345:1365	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	6	30	attach	presence	1322:1329	arg1	bath					1382:1385	coagulation bath	1370:1385	coagulation bath	1370:1385	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	6	30	attach	presence	1322:1329	arg2	glucose					1334:1340	glucose	1334:1340	glucose	1334:1340	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	3	31	theme	glucose	537:543	arg1	presence					525:532	The presence	521:532	The presence of glucose	521:543	The presence of glucose can seriously affect the performance of the produced RCFs and hinder their applications, so it has become critical to clarify the regulation and mechanism of this process.					
36867861	5	32	from	solution	965:972	arg1	effect					927:932	The effect	923:932	The effect of glucose content in spinning solution on fiber spinnability	923:994	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	1	33	theme	outstanding	177:187	arg1	properties					200:209	outstanding mechanical properties	177:209	outstanding mechanical properties	177:209	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	3	34	theme	process	708:714	arg1	mechanism					690:698	mechanism	690:698	mechanism	690:698	The presence of glucose can seriously affect the performance of the produced RCFs and hinder their applications, so it has become critical to clarify the regulation and mechanism of this process.					
36867861	3	34	theme	process	708:714	arg1	regulation					675:684	regulation	675:684	regulation	675:684	The presence of glucose can seriously affect the performance of the produced RCFs and hinder their applications, so it has become critical to clarify the regulation and mechanism of this process.					
36867861	6	35	theme	RCFs	1294:1297	arg1	factor					1284:1289	orientation factor	1272:1289	orientation factor	1272:1289	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	6	35	theme	RCFs	1294:1297	arg1	crystallinity					1253:1265	crystallinity	1253:1265	crystallinity	1253:1265	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	6	35	theme	RCFs	1294:1297	arg1	morphology					1241:1250	morphology	1241:1250	morphology	1241:1250	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	6	36	theme	practical	1467:1475	arg1	reference					1477:1485	practical reference and guidance	1467:1498	reference	1477:1485	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	1	37	theme	mechanical	189:198	arg1	properties					200:209	outstanding mechanical properties	177:209	outstanding mechanical properties	177:209	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	0	38	theme	Glucose	11:17	arg1	Effects					0:6	Effects	0:6	Effects of Glucose and Coagulant on the Structure and Properties of Regenerated Cellulose Fibers	0:95	Effects of Glucose and Coagulant on the Structure and Properties of Regenerated Cellulose Fibers.					
36867861	1	39	theme	applications	271:282	arg1	number					261:266	a large number	253:266	a large number of applications	253:282	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	1	40	theme	Regenerated	98:108	arg1	RCF					127:129	RCF	127:129	RCF	127:129	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	1	40	theme	Regenerated	98:108	arg1	fiber					120:124	Regenerated cellulose fiber	98:124	Regenerated cellulose fiber (RCF)	98:130	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	1	40	theme	Regenerated	98:108	arg1	material					163:170	an environmentally friendly material	135:170	an environmentally friendly material	135:170	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	5	41	theme	mechanical	1149:1158	arg1	properties					1160:1169	mechanical properties	1149:1169	mechanical properties	1149:1169	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	2	42	theme	coagulation	503:513	arg1	bath					515:518	coagulation bath	503:518	coagulation bath	503:518	However, during the spinning process using ionic liquids (ILs) as solvents, the dissolved cellulose continues to degrade and even produces degradation products such as glucose, which can enter the recycled solvent and coagulation bath.					
36867861	5	43	theme	rheological	1016:1026	arg1	analysis					1028:1035	rheological analysis	1016:1035	rheological analysis	1016:1035	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	0	44	dep	Structure	40:48	arg1	the					36:38	the	36:38	the	36:38	Effects of Glucose and Coagulant on the Structure and Properties of Regenerated Cellulose Fibers.					
36867861	4	45	dep	[Emim	779:783	arg1	DEP					785:787	DEP	785:787	DEP	785:787	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	0	46	theme	Coagulant	23:31	arg1	Effects					0:6	Effects	0:6	Effects of Glucose and Coagulant on the Structure and Properties of Regenerated Cellulose Fibers	0:95	Effects of Glucose and Coagulant on the Structure and Properties of Regenerated Cellulose Fibers.					
36867861	5	47	theme	glucose	1092:1098	arg1	content					1100:1106	glucose content	1092:1106	glucose content	1092:1106	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	6	48	theme	orientation	1272:1282	arg1	factor					1284:1289	orientation factor	1272:1289	orientation factor	1272:1289	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	3	49	theme	RCFs	598:601	arg1	performance					570:580	the performance	566:580	the performance of the produced RCFs	566:601	The presence of glucose can seriously affect the performance of the produced RCFs and hinder their applications, so it has become critical to clarify the regulation and mechanism of this process.					
36867861	5	50	theme	spinning	956:963	arg1	solution					965:972	spinning solution	956:972	spinning solution	956:972	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	2	51	theme	dissolved	365:373	arg1	cellulose					375:383	the dissolved cellulose	361:383	the dissolved cellulose	361:383	However, during the spinning process using ionic liquids (ILs) as solvents, the dissolved cellulose continues to degrade and even produces degradation products such as glucose, which can enter the recycled solvent and coagulation bath.					
36867861	6	52	theme	spinning	1349:1356	arg1	solution					1358:1365	the spinning solution	1345:1365	the spinning solution	1345:1365	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	5	53	theme	content	1100:1106	arg1	influence					1046:1054	the influence	1042:1054	the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs	1042:1181	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	4	54	theme	wood	847:850	arg1	WPC					868:870	WPC	868:870	WPC	868:870	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	4	54	theme	wood	847:850	arg1	cellulose					857:865	wood pulp cellulose	847:865	wood pulp cellulose (WPC)	847:871	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	1	55	used	used	245:248	arg2	fiber					120:124	Regenerated cellulose fiber	98:124	Regenerated cellulose fiber (RCF)	98:130	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	1	55	used	used	245:248	arg2	material					163:170	an environmentally friendly material	135:170	an environmentally friendly material	135:170	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	1	56	theme	cellulose	110:118	arg1	RCF					127:129	RCF	127:129	RCF	127:129	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	1	56	theme	cellulose	110:118	arg1	fiber					120:124	Regenerated cellulose fiber	98:124	Regenerated cellulose fiber (RCF)	98:130	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	1	56	theme	cellulose	110:118	arg1	material					163:170	an environmentally friendly material	135:170	an environmentally friendly material	135:170	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	2	57	theme	degradation	424:434	arg1	products					436:443	degradation products	424:443	degradation products such as glucose, which can enter the recycled solvent and coagulation bath	424:518	However, during the spinning process using ionic liquids (ILs) as solvents, the dissolved cellulose continues to degrade and even produces degradation products such as glucose, which can enter the recycled solvent and coagulation bath.					
36867861	2	57	theme	degradation	424:434	arg1	glucose					453:459	glucose	453:459	glucose	453:459	However, during the spinning process using ionic liquids (ILs) as solvents, the dissolved cellulose continues to degrade and even produces degradation products such as glucose, which can enter the recycled solvent and coagulation bath.					
36867861	3	58	dep	regulation	675:684	arg1	the					671:673	the	671:673	the	671:673	The presence of glucose can seriously affect the performance of the produced RCFs and hinder their applications, so it has become critical to clarify the regulation and mechanism of this process.					
36867861	5	59	theme	fiber	977:981	arg1	spinnability					983:994	fiber spinnability	977:994	fiber spinnability	977:994	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	6	60	theme	fiber	1542:1546	arg1	production					1519:1528	the industrial production	1504:1528	the industrial production of new type fiber	1504:1546	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	5	61	theme	content	945:951	arg1	effect					927:932	The effect	923:932	The effect of glucose content in spinning solution on fiber spinnability	923:994	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	4	62	theme	1-ethyl-3-methylimidazolium	732:758	arg1	[Emim					779:783	[Emim	779:783	[Emim	779:783	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	4	62	theme	1-ethyl-3-methylimidazolium	732:758	arg1	phosphate					768:776	1-ethyl-3-methylimidazolium diethyl phosphate	732:776	1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents	732:820	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	6	63	theme	type	1537:1540	arg1	fiber					1542:1546	new type fiber	1533:1546	new type fiber	1533:1546	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	1	64	with	material	163:170	arg1	properties					200:209	outstanding mechanical properties	177:209	outstanding mechanical properties	177:209	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	1	64	with	material	163:170	arg1	recyclability					215:227	recyclability	215:227	recyclability	215:227	Regenerated cellulose fiber (RCF) is an environmentally friendly material with outstanding mechanical properties and recyclability, which has been used in a large number of applications.					
36867861	3	65	theme	produced	589:596	arg1	RCFs					598:601	the produced RCFs	585:601	the produced RCFs	585:601	The presence of glucose can seriously affect the performance of the produced RCFs and hinder their applications, so it has become critical to clarify the regulation and mechanism of this process.					
36867861	6	66	theme	new	1533:1535	arg1	fiber					1542:1546	new type fiber	1533:1546	new type fiber	1533:1546	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	2	67	theme	spinning	305:312	arg1	process					314:320	the spinning process	301:320	the spinning process using ionic liquids (ILs) as solvents	301:358	However, during the spinning process using ionic liquids (ILs) as solvents, the dissolved cellulose continues to degrade and even produces degradation products such as glucose, which can enter the recycled solvent and coagulation bath.					
36867861	5	68	theme	glucose	937:943	arg1	content					945:951	glucose content	937:951	glucose content in spinning solution	937:972	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	6	69	theme	mechanical	1426:1435	arg1	properties					1437:1446	mechanical properties	1426:1446	mechanical properties	1426:1446	The results indicated that the morphology, crystallinity, and orientation factor of RCFs were influenced by the presence of glucose in the spinning solution or coagulation bath, resulting in corresponding changes in mechanical properties, which can provide practical reference and guidance for the industrial production of new type fiber.					
36867861	4	70	theme	coagulation	904:914	arg1	baths					916:920	different coagulation baths	894:920	different coagulation baths	894:920	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	5	71	theme	coagulation	1059:1069	arg1	composition					1076:1086	coagulation bath composition	1059:1086	coagulation bath composition	1059:1086	The effect of glucose content in spinning solution on fiber spinnability was investigated by rheological analysis, and the influence of coagulation bath composition and glucose content on the morphological characteristics and mechanical properties of the RCFs was also studied in depth.					
36867861	4	72	theme	diethyl	760:766	arg1	[Emim					779:783	[Emim	779:783	[Emim	779:783	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	4	72	theme	diethyl	760:766	arg1	phosphate					768:776	1-ethyl-3-methylimidazolium diethyl phosphate	732:776	1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents	732:820	In this study, 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) with different glucose contents was selected to dissolve wood pulp cellulose (WPC) and obtained RCFs in different coagulation baths.					
36867861	2	73	theme	recycled	482:489	arg1	solvent					491:497	the recycled solvent	478:497	the recycled solvent	478:497	However, during the spinning process using ionic liquids (ILs) as solvents, the dissolved cellulose continues to degrade and even produces degradation products such as glucose, which can enter the recycled solvent and coagulation bath.					
36235828	4	0	dep	cells	874:878	arg1	PBMCs					927:931	PBMCs	927:931	PBMCs	927:931	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	0	dep	cells	874:878	arg1	cells					920:924	peripheral blood mononuclear cells	891:924	peripheral blood mononuclear cells (PBMCs)	891:932	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	0	dep	cells	874:878	arg1	HT-29					881:885	HT-29	881:885	HT-29	881:885	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	1	theme	P.	683:684	arg1	strains					697:703	14 unrelated P. distasonis strains	670:703	14 unrelated P. distasonis strains	670:703	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	1	theme	P.	683:684	arg1	resistance					716:725	resistance	716:725	resistance to gastric conditions	716:747	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	1	theme	P.	683:684	arg1	immunomodulation					823:838	immunomodulation	823:838	immunomodulation	823:838	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	1	theme	P.	683:684	arg1	resistance					792:801	transepithelial resistance	776:801	transepithelial resistance (Caco-2 model)	776:816	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	1	theme	P.	683:684	arg1	adherence					750:758	adherence	750:758	adherence (Caco-2 model)	750:773	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	6	2	dep	induced	1108:1114	arg1	counteracted					1184:1195	counteracted	1184:1195	was counteracted by the overproduction of the anti-inflammatory cytokines	1180:1252	However, all the P. distasonis strains induced the overproduction of proinflammatory cytokines on PBMCs, which was counteracted by the overproduction of the anti-inflammatory cytokines.					
36235828	3	3	theme	much	511:514	arg1	attention					516:524	much attention	511:524	much attention due to the numerous beneficial properties it brings to its host	511:588	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	4	4	theme	unrelated	673:681	arg1	strains					697:703	14 unrelated P. distasonis strains	670:703	14 unrelated P. distasonis strains	670:703	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	4	theme	unrelated	673:681	arg1	resistance					716:725	resistance	716:725	resistance to gastric conditions	716:747	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	4	theme	unrelated	673:681	arg1	immunomodulation					823:838	immunomodulation	823:838	immunomodulation	823:838	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	4	theme	unrelated	673:681	arg1	resistance					792:801	transepithelial resistance	776:801	transepithelial resistance (Caco-2 model)	776:816	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	4	theme	unrelated	673:681	arg1	adherence					750:758	adherence	750:758	adherence (Caco-2 model)	750:773	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	7	5	theme	detrimental	1458:1468	arg1	effect					1470:1475	no detrimental effect	1455:1475	no detrimental effect on the host	1455:1487	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	3	6	theme	due	526:528	arg1	attention					516:524	much attention	511:524	much attention due to the numerous beneficial properties it brings to its host	511:588	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	3	7	theme	beneficial	546:555	arg1	properties					557:566	the numerous beneficial properties	533:566	the numerous beneficial properties it brings to its host	533:588	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	4	8	theme	NBPs	648:651	arg1	selection					635:643	the selection	631:643	the selection of NBPs	631:651	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	9	theme	nontreated	844:853	arg1	cells					874:878	nontreated and LPS-stimulated cells	844:878	nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs))	844:933	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	2	10	theme	numerous	295:302	arg1	studies					304:310	numerous studies	295:310	numerous studies	295:310	In order to counterbalance host disorder caused by an alteration in the gut composition, numerous studies have focused on identifying new biotherapeutic products (NBPs).					
36235828	3	11	dep	properties	557:566	arg1	brings					571:576	brings	571:576	brings to its host	571:588	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	6	12	theme	cytokines	1244:1252	arg1	overproduction					1204:1217	the overproduction	1200:1217	the overproduction of the anti-inflammatory cytokines	1200:1252	However, all the P. distasonis strains induced the overproduction of proinflammatory cytokines on PBMCs, which was counteracted by the overproduction of the anti-inflammatory cytokines.					
36235828	0	13	attach	Linked	38:43	arg1	Selection					52:60	the Selection	48:60	the Selection of New Biotherapeutics	48:83	Parabacteroides distasonis Properties Linked to the Selection of New Biotherapeutics.					
36235828	0	13	attach	Linked	38:43	arg2	Properties					27:36	Parabacteroides distasonis Properties	0:36	Parabacteroides distasonis Properties	0:36	Parabacteroides distasonis Properties Linked to the Selection of New Biotherapeutics.					
36235828	4	14	theme	Caco-2	761:766	arg1	adherence					750:758	adherence	750:758	adherence (Caco-2 model)	750:773	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	14	theme	Caco-2	761:766	arg1	model					768:772	Caco-2 model	761:772	Caco-2 model	761:772	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	1	15	theme	ulcerative	186:195	arg1	colitis					197:203	ulcerative colitis	186:203	ulcerative colitis	186:203	Dysbiotic microbiota is often associated with health issues including inflammatory bowel disease or ulcerative colitis.					
36235828	6	16	theme	anti-inflammatory	1226:1242	arg1	cytokines					1244:1252	the anti-inflammatory cytokines	1222:1252	the anti-inflammatory cytokines	1222:1252	However, all the P. distasonis strains induced the overproduction of proinflammatory cytokines on PBMCs, which was counteracted by the overproduction of the anti-inflammatory cytokines.					
36235828	1	17	theme	Dysbiotic	86:94	arg1	microbiota					96:105	Dysbiotic microbiota	86:105	Dysbiotic microbiota	86:105	Dysbiotic microbiota is often associated with health issues including inflammatory bowel disease or ulcerative colitis.					
36235828	0	18	theme	distasonis	16:25	arg1	Properties					27:36	Parabacteroides distasonis Properties	0:36	Parabacteroides distasonis Properties	0:36	Parabacteroides distasonis Properties Linked to the Selection of New Biotherapeutics.					
36235828	3	19	theme	gut	434:436	arg1	distasonis					420:429	Parabacteroides distasonis	404:429	Parabacteroides distasonis	404:429	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	3	19	theme	gut	434:436	arg1	part					456:459	a gut microbiota member part	432:459	a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host	432:588	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	0	20	theme	Parabacteroides	0:14	arg1	Properties					27:36	Parabacteroides distasonis Properties	0:36	Parabacteroides distasonis Properties	0:36	Parabacteroides distasonis Properties Linked to the Selection of New Biotherapeutics.					
36235828	4	21	attach	linked	621:626	arg2	properties					610:619	the properties	606:619	the properties linked to the selection of NBPs	606:651	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	21	attach	linked	621:626	arg1	selection					635:643	the selection	631:643	the selection of NBPs	631:651	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	3	22	theme	microbiota	438:447	arg1	distasonis					420:429	Parabacteroides distasonis	404:429	Parabacteroides distasonis	404:429	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	3	22	theme	microbiota	438:447	arg1	part					456:459	a gut microbiota member part	432:459	a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host	432:588	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	7	23	theme	lowest	1390:1395	arg1	overproduction					1397:1410	the lowest overproduction	1386:1410	the lowest overproduction of proinflammatory cytokines on PBMCs	1386:1448	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	4	24	theme	peripheral	891:900	arg1	PBMCs					927:931	PBMCs	927:931	PBMCs	927:931	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	24	theme	peripheral	891:900	arg1	cells					920:924	peripheral blood mononuclear cells	891:924	peripheral blood mononuclear cells (PBMCs)	891:932	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	2	25	from	alteration	260:269	arg1	composition					282:292	the gut composition	274:292	the gut composition	274:292	In order to counterbalance host disorder caused by an alteration in the gut composition, numerous studies have focused on identifying new biotherapeutic products (NBPs).					
36235828	3	26	theme	core	468:471	arg1	microbiome					473:482	the core microbiome	464:482	the core microbiome	464:482	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	3	27	theme	promising	386:394	arg1	NBPs					396:399	the promising NBPs	382:399	the promising NBPs	382:399	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	6	28	theme	proinflammatory	1138:1152	arg1	cytokines					1154:1162	proinflammatory cytokines	1138:1162	proinflammatory cytokines	1138:1162	However, all the P. distasonis strains induced the overproduction of proinflammatory cytokines on PBMCs, which was counteracted by the overproduction of the anti-inflammatory cytokines.					
36235828	5	29	theme	in	1026:1027	arg1	properties					1050:1059	almost all the in vitro biotherapeutic properties	1011:1059	almost all the in vitro biotherapeutic properties tested	1011:1066	This approach allowed for the identification of five strains that combined almost all the in vitro biotherapeutic properties tested.					
36235828	4	30	theme	gastric	730:736	arg1	conditions					738:747	gastric conditions	730:747	gastric conditions	730:747	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	6	31	from	overproduction	1120:1133	arg1	PBMCs					1167:1171	PBMCs	1167:1171	PBMCs	1167:1171	However, all the P. distasonis strains induced the overproduction of proinflammatory cytokines on PBMCs, which was counteracted by the overproduction of the anti-inflammatory cytokines.					
36235828	3	32	theme	microbiome	473:482	arg1	distasonis					420:429	Parabacteroides distasonis	404:429	Parabacteroides distasonis	404:429	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	3	32	theme	microbiome	473:482	arg1	part					456:459	a gut microbiota member part	432:459	a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host	432:588	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	4	33	theme	distasonis	686:695	arg1	strains					697:703	14 unrelated P. distasonis strains	670:703	14 unrelated P. distasonis strains	670:703	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	33	theme	distasonis	686:695	arg1	resistance					716:725	resistance	716:725	resistance to gastric conditions	716:747	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	33	theme	distasonis	686:695	arg1	immunomodulation					823:838	immunomodulation	823:838	immunomodulation	823:838	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	33	theme	distasonis	686:695	arg1	resistance					792:801	transepithelial resistance	776:801	transepithelial resistance (Caco-2 model)	776:816	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	33	theme	distasonis	686:695	arg1	adherence					750:758	adherence	750:758	adherence (Caco-2 model)	750:773	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	34	theme	mononuclear	908:918	arg1	PBMCs					927:931	PBMCs	927:931	PBMCs	927:931	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	34	theme	mononuclear	908:918	arg1	cells					920:924	peripheral blood mononuclear cells	891:924	peripheral blood mononuclear cells (PBMCs)	891:932	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	7	35	theme	strong	1352:1357	arg1	function					1376:1383	strong health-promoting function	1352:1383	strong health-promoting function	1352:1383	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	7	36	theme	cytokines	1431:1439	arg1	function					1376:1383	strong health-promoting function	1352:1383	strong health-promoting function	1352:1383	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	7	36	theme	cytokines	1431:1439	arg1	effect					1470:1475	no detrimental effect	1455:1475	no detrimental effect on the host	1455:1487	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	7	36	theme	cytokines	1431:1439	arg1	overproduction					1397:1410	the lowest overproduction	1386:1410	the lowest overproduction of proinflammatory cytokines on PBMCs	1386:1448	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	3	37	theme	member	449:454	arg1	distasonis					420:429	Parabacteroides distasonis	404:429	Parabacteroides distasonis	404:429	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	3	37	theme	member	449:454	arg1	part					456:459	a gut microbiota member part	432:459	a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host	432:588	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	4	38	theme	blood	902:906	arg1	PBMCs					927:931	PBMCs	927:931	PBMCs	927:931	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	38	theme	blood	902:906	arg1	cells					920:924	peripheral blood mononuclear cells	891:924	peripheral blood mononuclear cells (PBMCs)	891:932	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	5	39	theme	biotherapeutic	1035:1048	arg1	properties					1050:1059	almost all the in vitro biotherapeutic properties	1011:1059	almost all the in vitro biotherapeutic properties tested	1011:1066	This approach allowed for the identification of five strains that combined almost all the in vitro biotherapeutic properties tested.					
36235828	7	40	theme	health-promoting	1359:1374	arg1	function					1376:1383	strong health-promoting function	1352:1383	strong health-promoting function	1352:1383	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	3	41	theme	Parabacteroides	404:418	arg1	distasonis					420:429	Parabacteroides distasonis	404:429	Parabacteroides distasonis	404:429	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	3	41	theme	Parabacteroides	404:418	arg1	part					456:459	a gut microbiota member part	432:459	a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host	432:588	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	5	42	theme	strains	989:995	arg1	identification					966:979	the identification	962:979	the identification of five strains that combined almost all the in vitro biotherapeutic properties tested	962:1066	This approach allowed for the identification of five strains that combined almost all the in vitro biotherapeutic properties tested.					
36235828	6	43	theme	distasonis	1089:1098	arg1	strains					1100:1106	all the P. distasonis strains	1078:1106	all the P. distasonis strains	1078:1106	However, all the P. distasonis strains induced the overproduction of proinflammatory cytokines on PBMCs, which was counteracted by the overproduction of the anti-inflammatory cytokines.					
36235828	6	44	theme	cytokines	1154:1162	arg1	overproduction					1120:1133	the overproduction	1116:1133	the overproduction of proinflammatory cytokines on PBMCs	1116:1171	However, all the P. distasonis strains induced the overproduction of proinflammatory cytokines on PBMCs, which was counteracted by the overproduction of the anti-inflammatory cytokines.					
36235828	4	45	theme	LPS-stimulated	859:872	arg1	cells					874:878	nontreated and LPS-stimulated cells	844:878	nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs))	844:933	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	2	46	theme	host	233:236	arg1	disorder					238:245	host disorder	233:245	host disorder caused by an alteration in the gut composition	233:292	In order to counterbalance host disorder caused by an alteration in the gut composition, numerous studies have focused on identifying new biotherapeutic products (NBPs).					
36235828	4	47	theme	Caco-2	804:809	arg1	resistance					792:801	transepithelial resistance	776:801	transepithelial resistance (Caco-2 model)	776:816	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	47	theme	Caco-2	804:809	arg1	model					811:815	Caco-2 model	804:815	Caco-2 model	804:815	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	1	48	theme	health	132:137	arg1	issues					139:144	health issues	132:144	health issues including inflammatory bowel disease or ulcerative colitis	132:203	Dysbiotic microbiota is often associated with health issues including inflammatory bowel disease or ulcerative colitis.					
36235828	1	48	theme	health	132:137	arg1	disease					175:181	inflammatory bowel disease	156:181	inflammatory bowel disease	156:181	Dysbiotic microbiota is often associated with health issues including inflammatory bowel disease or ulcerative colitis.					
36235828	1	48	theme	health	132:137	arg1	colitis					197:203	ulcerative colitis	186:203	ulcerative colitis	186:203	Dysbiotic microbiota is often associated with health issues including inflammatory bowel disease or ulcerative colitis.					
36235828	7	49	from	function	1376:1383	arg1	host					1484:1487	the host	1480:1487	the host	1480:1487	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	7	49	from	function	1376:1383	arg1	PBMCs					1444:1448	PBMCs	1444:1448	PBMCs	1444:1448	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	2	50	theme	gut	278:280	arg1	composition					282:292	the gut composition	274:292	the gut composition	274:292	In order to counterbalance host disorder caused by an alteration in the gut composition, numerous studies have focused on identifying new biotherapeutic products (NBPs).					
36235828	2	51	theme	biotherapeutic	344:357	arg1	products					359:366	new biotherapeutic products	340:366	new biotherapeutic products (NBPs)	340:373	In order to counterbalance host disorder caused by an alteration in the gut composition, numerous studies have focused on identifying new biotherapeutic products (NBPs).					
36235828	2	51	theme	biotherapeutic	344:357	arg1	NBPs					369:372	NBPs	369:372	NBPs	369:372	In order to counterbalance host disorder caused by an alteration in the gut composition, numerous studies have focused on identifying new biotherapeutic products (NBPs).					
36235828	3	52	theme	numerous	537:544	arg1	properties					557:566	the numerous beneficial properties	533:566	the numerous beneficial properties it brings to its host	533:588	Among the promising NBPs is Parabacteroides distasonis, a gut microbiota member part of the core microbiome that recently has received much attention due to the numerous beneficial properties it brings to its host.					
36235828	7	53	theme	proinflammatory	1415:1429	arg1	cytokines					1431:1439	proinflammatory cytokines	1415:1439	proinflammatory cytokines	1415:1439	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	2	54	theme	new	340:342	arg1	products					359:366	new biotherapeutic products	340:366	new biotherapeutic products (NBPs)	340:373	In order to counterbalance host disorder caused by an alteration in the gut composition, numerous studies have focused on identifying new biotherapeutic products (NBPs).					
36235828	2	54	theme	new	340:342	arg1	NBPs					369:372	NBPs	369:372	NBPs	369:372	In order to counterbalance host disorder caused by an alteration in the gut composition, numerous studies have focused on identifying new biotherapeutic products (NBPs).					
36235828	0	55	theme	Biotherapeutics	69:83	arg1	Selection					52:60	the Selection	48:60	the Selection of New Biotherapeutics	48:83	Parabacteroides distasonis Properties Linked to the Selection of New Biotherapeutics.					
36235828	4	56	theme	transepithelial	776:790	arg1	resistance					792:801	transepithelial resistance	776:801	transepithelial resistance (Caco-2 model)	776:816	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	4	56	theme	transepithelial	776:790	arg1	model					811:815	Caco-2 model	804:815	Caco-2 model	804:815	In this study, the properties linked to the selection of NBPs were screened in 14 unrelated P. distasonis strains, including resistance to gastric conditions, adherence (Caco-2 model), transepithelial resistance (Caco-2 model), and immunomodulation, on nontreated and LPS-stimulated cells (HT-29 and peripheral blood mononuclear cells (PBMCs)).					
36235828	1	57	theme	inflammatory	156:167	arg1	disease					175:181	inflammatory bowel disease	156:181	inflammatory bowel disease	156:181	Dysbiotic microbiota is often associated with health issues including inflammatory bowel disease or ulcerative colitis.					
36235828	5	58	dep	in	1026:1027	arg1	vitro					1029:1033	vitro	1029:1033	vitro	1029:1033	This approach allowed for the identification of five strains that combined almost all the in vitro biotherapeutic properties tested.					
36235828	0	59	theme	New	65:67	arg1	Biotherapeutics					69:83	New Biotherapeutics	65:83	New Biotherapeutics	65:83	Parabacteroides distasonis Properties Linked to the Selection of New Biotherapeutics.					
36235828	7	60	from	effect	1470:1475	arg1	host					1484:1487	the host	1480:1487	the host	1480:1487	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	7	60	from	effect	1470:1475	arg1	PBMCs					1444:1448	PBMCs	1444:1448	PBMCs	1444:1448	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	7	61	theme	potential	1326:1334	arg1	NBP					1336:1338	a potential NBP	1324:1338	a potential NBP	1324:1338	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	7	61	theme	potential	1326:1334	arg1	attention					1311:1319	our attention	1307:1319	our attention	1307:1319	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	1	62	theme	bowel	169:173	arg1	disease					175:181	inflammatory bowel disease	156:181	inflammatory bowel disease	156:181	Dysbiotic microbiota is often associated with health issues including inflammatory bowel disease or ulcerative colitis.					
36235828	7	63	from	overproduction	1397:1410	arg1	host					1484:1487	the host	1480:1487	the host	1480:1487	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36235828	7	63	from	overproduction	1397:1410	arg1	PBMCs					1444:1448	PBMCs	1444:1448	PBMCs	1444:1448	Among these five strains, two particularly retained our attention as a potential NBP, by showing strong health-promoting function, the lowest overproduction of proinflammatory cytokines on PBMCs, and no detrimental effect on the host.					
36366189	3	0	theme	pulse	655:659	arg1	DPV					674:676	DPV	674:676	DPV	674:676	The sensor, termed MIP@CNT/CNC MN, was analyzed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and showed excellent electrochemical performance for the detection of IDP.					
36366189	3	0	theme	pulse	655:659	arg1	voltammetry					661:671	differential pulse voltammetry	642:671	differential pulse voltammetry (DPV)	642:677	The sensor, termed MIP@CNT/CNC MN, was analyzed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and showed excellent electrochemical performance for the detection of IDP.					
36366189	1	1	theme	MIP	163:165	arg1	sensor					191:196	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor	120:196	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP)	120:252	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP) has been demonstrated.					
36366189	5	2	theme	excellent	959:967	arg1	reusability					969:979	excellent reusability	959:979	excellent reusability	959:979	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	2	3	theme	polyaniline	437:447	arg1	nanotube					362:369	a carbon nanotube	353:369	a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite	353:413	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	3	theme	polyaniline	437:447	arg1	film					549:552	the biomimetic receptor film	525:552	the biomimetic receptor film	525:552	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	3	theme	polyaniline	437:447	arg1	layer					449:453	an IDP-imprinted polyaniline layer	420:453	an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im)	420:520	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	5	4	theme	MIP	930:932	arg1	sensor					945:950	the MIP@CNT/CNC MN sensor	926:950	the MIP@CNT/CNC MN sensor	926:950	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	6	5	theme	@	1094:1094	arg1	sensor					1106:1111	the MIP@CNT/CNC MN sensor	1087:1111	the MIP@CNT/CNC MN sensor	1087:1111	Lastly, the MIP@CNT/CNC MN sensor successfully demonstrated the quantification of IDP in a honey sample.					
36366189	6	6	theme	CNT/CNC	1095:1101	arg1	sensor					1106:1111	the MIP@CNT/CNC MN sensor	1087:1111	the MIP@CNT/CNC MN sensor	1087:1111	Lastly, the MIP@CNT/CNC MN sensor successfully demonstrated the quantification of IDP in a honey sample.					
36366189	5	7	used	used	994:997	arg2	sensor					945:950	the MIP@CNT/CNC MN sensor	926:950	the MIP@CNT/CNC MN sensor	926:950	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	0	8	theme	Food	106:109	arg1	Samples					111:117	Food Samples	106:117	Food Samples	106:117	Molecularly Imprinted Polymer-Modified Microneedle Sensor for the Detection of Imidacloprid Pesticides in Food Samples.					
36366189	0	9	from	Detection	66:74	arg1	Samples					111:117	Food Samples	106:117	Food Samples	106:117	Molecularly Imprinted Polymer-Modified Microneedle Sensor for the Detection of Imidacloprid Pesticides in Food Samples.					
36366189	4	10	theme	µM	908:909	arg1	LOD					896:898	an LOD	893:898	an LOD of 0.06 µM	893:909	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	2	11	theme	in-tube	331:337	arg1	coating					339:345	layer-by-layer (LbL) in-tube coating	310:345	layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film	310:552	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	4	12	theme	detection	857:865	arg1	range					867:871	the DPV detection range	849:871	the DPV detection range	849:871	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	4	12	theme	detection	857:865	arg1	µM					885:886	0.20-92 µM	877:886	0.20-92 µM	877:886	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	2	13	theme	LbL	326:328	arg1	coating					339:345	layer-by-layer (LbL) in-tube coating	310:345	layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film	310:552	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	6	14	theme	MIP	1091:1093	arg1	sensor					1106:1111	the MIP@CNT/CNC MN sensor	1087:1111	the MIP@CNT/CNC MN sensor	1087:1111	Lastly, the MIP@CNT/CNC MN sensor successfully demonstrated the quantification of IDP in a honey sample.					
36366189	1	15	dep	portable	122:129	arg1	imprinted					144:152	imprinted	144:152	imprinted	144:152	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP) has been demonstrated.					
36366189	1	16	theme	-based	167:172	arg1	sensor					191:196	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor	120:196	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP)	120:252	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP) has been demonstrated.					
36366189	2	17	theme	imidazole-functionalized	475:498	arg1	CNCs					500:503	imidazole-functionalized CNCs	475:503	imidazole-functionalized CNCs (PANI-co-CNC-Im)	475:520	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	17	theme	imidazole-functionalized	475:498	arg1	PANI-co-CNC-Im					506:519	PANI-co-CNC-Im	506:519	PANI-co-CNC-Im	506:519	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	5	18	dep	nine	1005:1008	arg1	to					1002:1003	to	1002:1003	to	1002:1003	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	6	19	theme	honey	1170:1174	arg1	sample					1176:1181	a honey sample	1168:1181	a honey sample	1168:1181	Lastly, the MIP@CNT/CNC MN sensor successfully demonstrated the quantification of IDP in a honey sample.					
36366189	1	20	theme	microneedle	174:184	arg1	sensor					191:196	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor	120:196	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP)	120:252	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP) has been demonstrated.					
36366189	2	21	theme	composite	405:413	arg1	nanotube					362:369	a carbon nanotube	353:369	a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite	353:413	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	21	theme	composite	405:413	arg1	film					549:552	the biomimetic receptor film	525:552	the biomimetic receptor film	525:552	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	21	theme	composite	405:413	arg1	layer					449:453	an IDP-imprinted polyaniline layer	420:453	an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im)	420:520	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	21	theme	composite	405:413	arg1	CNT					372:374	CNT	372:374	CNT	372:374	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	0	22	theme	Pesticides	92:101	arg1	Detection					66:74	the Detection	62:74	the Detection of Imidacloprid Pesticides in Food Samples	62:117	Molecularly Imprinted Polymer-Modified Microneedle Sensor for the Detection of Imidacloprid Pesticides in Food Samples.					
36366189	5	23	theme	1.4	1023:1025	arg1	%					1027:1027	%	1027:1027	%	1027:1027	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	2	24	theme	carbon	355:360	arg1	nanotube					362:369	a carbon nanotube	353:369	a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite	353:413	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	24	theme	carbon	355:360	arg1	film					549:552	the biomimetic receptor film	525:552	the biomimetic receptor film	525:552	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	24	theme	carbon	355:360	arg1	layer					449:453	an IDP-imprinted polyaniline layer	420:453	an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im)	420:520	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	24	theme	carbon	355:360	arg1	CNT					372:374	CNT	372:374	CNT	372:374	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	3	25	theme	excellent	690:698	arg1	performance					716:726	excellent electrochemical performance	690:726	excellent electrochemical performance for the detection of IDP	690:751	The sensor, termed MIP@CNT/CNC MN, was analyzed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and showed excellent electrochemical performance for the detection of IDP.					
36366189	1	26	theme	MN	187:188	arg1	sensor					191:196	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor	120:196	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP)	120:252	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP) has been demonstrated.					
36366189	0	27	theme	Microneedle	39:49	arg1	Sensor					51:56	Polymer-Modified Microneedle Sensor	22:56	Polymer-Modified Microneedle Sensor for the Detection of Imidacloprid Pesticides in Food Samples	22:117	Molecularly Imprinted Polymer-Modified Microneedle Sensor for the Detection of Imidacloprid Pesticides in Food Samples.					
36366189	3	28	theme	electrochemical	700:714	arg1	performance					716:726	excellent electrochemical performance	690:726	excellent electrochemical performance for the detection of IDP	690:751	The sensor, termed MIP@CNT/CNC MN, was analyzed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and showed excellent electrochemical performance for the detection of IDP.					
36366189	2	29	theme	biomimetic	529:538	arg1	nanotube					362:369	a carbon nanotube	353:369	a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite	353:413	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	29	theme	biomimetic	529:538	arg1	film					549:552	the biomimetic receptor film	525:552	the biomimetic receptor film	525:552	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	29	theme	biomimetic	529:538	arg1	layer					449:453	an IDP-imprinted polyaniline layer	420:453	an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im)	420:520	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	0	30	theme	Polymer-Modified	22:37	arg1	Sensor					51:56	Polymer-Modified Microneedle Sensor	22:56	Polymer-Modified Microneedle Sensor for the Detection of Imidacloprid Pesticides in Food Samples	22:117	Molecularly Imprinted Polymer-Modified Microneedle Sensor for the Detection of Imidacloprid Pesticides in Food Samples.					
36366189	5	31	theme	relative	1029:1036	arg1	deviation					1047:1055	a 1.4 % relative standard deviation	1021:1055	a 1.4 % relative standard deviation (% RSD) between uses	1021:1076	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	5	31	theme	relative	1029:1036	arg1	RSD					1060:1062	% RSD	1058:1062	% RSD	1058:1062	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	5	32	theme	CNT/CNC	934:940	arg1	sensor					945:950	the MIP@CNT/CNC MN sensor	926:950	the MIP@CNT/CNC MN sensor	926:950	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	2	33	theme	MN	281:282	arg1	sensor					284:289	The MN sensor	277:289	The MN sensor	277:289	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	34	theme	IDP-imprinted	423:435	arg1	nanotube					362:369	a carbon nanotube	353:369	a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite	353:413	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	34	theme	IDP-imprinted	423:435	arg1	film					549:552	the biomimetic receptor film	525:552	the biomimetic receptor film	525:552	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	34	theme	IDP-imprinted	423:435	arg1	layer					449:453	an IDP-imprinted polyaniline layer	420:453	an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im)	420:520	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	5	35	theme	standard	1038:1045	arg1	deviation					1047:1055	a 1.4 % relative standard deviation	1021:1055	a 1.4 % relative standard deviation (% RSD) between uses	1021:1076	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	5	35	theme	standard	1038:1045	arg1	RSD					1060:1062	% RSD	1058:1062	% RSD	1058:1062	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	6	36	theme	IDP	1161:1163	arg1	quantification					1143:1156	the quantification	1139:1156	the quantification of IDP	1139:1163	Lastly, the MIP@CNT/CNC MN sensor successfully demonstrated the quantification of IDP in a honey sample.					
36366189	5	37	theme	MN	942:943	arg1	sensor					945:950	the MIP@CNT/CNC MN sensor	926:950	the MIP@CNT/CNC MN sensor	926:950	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	4	38	theme	detection	761:769	arg1	µM					796:797	2.0-99 µM	789:797	2.0-99 µM	789:797	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	4	38	theme	detection	761:769	arg1	range					771:775	The CV detection range	754:775	The CV detection range for IDP	754:783	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	4	39	theme	µM	839:840	arg1	LOD					826:828	LOD	826:828	LOD	826:828	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	4	39	theme	µM	839:840	arg1	detection					815:823	detection	815:823	detection (LOD) of 0.35 µM	815:840	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	3	40	theme	MIP	574:576	arg1	MN					586:587	MIP@CNT/CNC MN	574:587	MIP@CNT/CNC MN	574:587	The sensor, termed MIP@CNT/CNC MN, was analyzed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and showed excellent electrochemical performance for the detection of IDP.					
36366189	4	41	with	µM	885:886	arg1	LOD					896:898	an LOD	893:898	an LOD of 0.06 µM	893:909	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	3	42	theme	IDP	749:751	arg1	detection					736:744	the detection	732:744	the detection of IDP	732:751	The sensor, termed MIP@CNT/CNC MN, was analyzed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and showed excellent electrochemical performance for the detection of IDP.					
36366189	5	43	theme	%	1027:1027	arg1	deviation					1047:1055	a 1.4 % relative standard deviation	1021:1055	a 1.4 % relative standard deviation (% RSD) between uses	1021:1076	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	5	43	theme	%	1027:1027	arg1	RSD					1060:1062	% RSD	1058:1062	% RSD	1058:1062	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	1	44	theme	portable	122:129	arg1	sensor					191:196	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor	120:196	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP)	120:252	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP) has been demonstrated.					
36366189	4	45	theme	detection	815:823	arg1	limits					805:810	limits	805:810	limits of detection (LOD) of 0.35 µM	805:840	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	6	46	theme	MN	1103:1104	arg1	sensor					1106:1111	the MIP@CNT/CNC MN sensor	1087:1111	the MIP@CNT/CNC MN sensor	1087:1111	Lastly, the MIP@CNT/CNC MN sensor successfully demonstrated the quantification of IDP in a honey sample.					
36366189	4	47	theme	CV	758:759	arg1	µM					796:797	2.0-99 µM	789:797	2.0-99 µM	789:797	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	4	47	theme	CV	758:759	arg1	range					771:775	The CV detection range	754:775	The CV detection range for IDP	754:783	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	2	48	theme	layer-by-layer	310:323	arg1	coating					339:345	layer-by-layer (LbL) in-tube coating	310:345	layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film	310:552	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	1	49	theme	electrochemical	206:220	arg1	detection					222:230	the electrochemical detection	202:230	the electrochemical detection of imidacloprid (IDP)	202:252	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP) has been demonstrated.					
36366189	2	50	theme	receptor	540:547	arg1	nanotube					362:369	a carbon nanotube	353:369	a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite	353:413	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	50	theme	receptor	540:547	arg1	film					549:552	the biomimetic receptor film	525:552	the biomimetic receptor film	525:552	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	2	50	theme	receptor	540:547	arg1	layer					449:453	an IDP-imprinted polyaniline layer	420:453	an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im)	420:520	The MN sensor was fabricated via layer-by-layer (LbL) in-tube coating using a carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite, and an IDP-imprinted polyaniline layer co-polymerized with imidazole-functionalized CNCs (PANI-co-CNC-Im) as the biomimetic receptor film.					
36366189	3	51	theme	cyclic	614:619	arg1	voltammetry					621:631	cyclic voltammetry	614:631	cyclic voltammetry (CV)	614:636	The sensor, termed MIP@CNT/CNC MN, was analyzed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and showed excellent electrochemical performance for the detection of IDP.					
36366189	3	51	theme	cyclic	614:619	arg1	CV					634:635	CV	634:635	CV	634:635	The sensor, termed MIP@CNT/CNC MN, was analyzed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and showed excellent electrochemical performance for the detection of IDP.					
36366189	0	52	theme	Imidacloprid	79:90	arg1	Pesticides					92:101	Imidacloprid Pesticides	79:101	Imidacloprid Pesticides	79:101	Molecularly Imprinted Polymer-Modified Microneedle Sensor for the Detection of Imidacloprid Pesticides in Food Samples.					
36366189	5	53	theme	@	933:933	arg1	sensor					945:950	the MIP@CNT/CNC MN sensor	926:950	the MIP@CNT/CNC MN sensor	926:950	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	3	54	theme	@	577:577	arg1	MN					586:587	MIP@CNT/CNC MN	574:587	MIP@CNT/CNC MN	574:587	The sensor, termed MIP@CNT/CNC MN, was analyzed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and showed excellent electrochemical performance for the detection of IDP.					
36366189	1	55	theme	polymer	154:160	arg1	sensor					191:196	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor	120:196	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP)	120:252	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP) has been demonstrated.					
36366189	5	56	theme	%	1058:1058	arg1	deviation					1047:1055	a 1.4 % relative standard deviation	1021:1055	a 1.4 % relative standard deviation (% RSD) between uses	1021:1076	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	5	56	theme	%	1058:1058	arg1	RSD					1060:1062	% RSD	1058:1062	% RSD	1058:1062	Additionally, the MIP@CNT/CNC MN sensor showed excellent reusability and could be used up to nine times with a 1.4 % relative standard deviation (% RSD) between uses.					
36366189	3	57	theme	differential	642:653	arg1	DPV					674:676	DPV	674:676	DPV	674:676	The sensor, termed MIP@CNT/CNC MN, was analyzed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and showed excellent electrochemical performance for the detection of IDP.					
36366189	3	57	theme	differential	642:653	arg1	voltammetry					661:671	differential pulse voltammetry	642:671	differential pulse voltammetry (DPV)	642:677	The sensor, termed MIP@CNT/CNC MN, was analyzed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and showed excellent electrochemical performance for the detection of IDP.					
36366189	3	58	theme	CNT/CNC	578:584	arg1	MN					586:587	MIP@CNT/CNC MN	574:587	MIP@CNT/CNC MN	574:587	The sensor, termed MIP@CNT/CNC MN, was analyzed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and showed excellent electrochemical performance for the detection of IDP.					
36366189	4	59	theme	DPV	853:855	arg1	range					867:871	the DPV detection range	849:871	the DPV detection range	849:871	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	4	59	theme	DPV	853:855	arg1	µM					885:886	0.20-92 µM	877:886	0.20-92 µM	877:886	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	4	60	with	µM	796:797	arg1	limits					805:810	limits	805:810	limits of detection (LOD) of 0.35 µM	805:840	The CV detection range for IDP was 2.0-99 µM, with limits of detection (LOD) of 0.35 µM, while the DPV detection range was 0.20-92 µM with an LOD of 0.06 µM.					
36366189	1	61	theme	imidacloprid	235:246	arg1	detection					222:230	the electrochemical detection	202:230	the electrochemical detection of imidacloprid (IDP)	202:252	A portable, molecularly imprinted polymer (MIP)-based microneedle (MN) sensor for the electrochemical detection of imidacloprid (IDP) has been demonstrated.					
37148940	8	0	theme	molecular	1091:1099	arg1	simulation					1109:1118	molecular docking simulation	1091:1118	molecular docking simulation	1091:1118	The AFM images and results of molecular docking simulation indicated that TP could affect the cell wall of bacteria by acting with peptidoglycan.					
37148940	4	1	theme	novel	484:488	arg1	film					500:503	A novel packaging film	482:503	A novel packaging film based on sodium alginate and konjac glucomannan (SA-KGM) blended with tea polyphenols (TP)	482:594	A novel packaging film based on sodium alginate and konjac glucomannan (SA-KGM) blended with tea polyphenols (TP) was prepared in the present study.					
37148940	8	2	theme	AFM	1065:1067	arg1	images					1069:1074	The AFM images	1061:1074	The AFM images	1061:1074	The AFM images and results of molecular docking simulation indicated that TP could affect the cell wall of bacteria by acting with peptidoglycan.					
37148940	9	3	theme	bioactive	1341:1349	arg1	material					1361:1368	a novel bioactive packaging material	1333:1368	a novel bioactive packaging material with wide application potential in food preservation	1333:1421	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	9	3	theme	bioactive	1341:1349	arg1	film					1319:1322	TP-SA-KGM film	1309:1322	TP-SA-KGM film	1309:1322	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	0	4	theme	tea	76:78	arg1	polyphenols					80:90	tea polyphenols	76:90	tea polyphenols for food preservation	76:112	Composite films of sodium alginate and konjac glucomannan incorporated with tea polyphenols for food preservation.					
37148940	6	5	theme	molecular	849:857	arg1	simulation					867:876	molecular docking simulation	849:876	molecular docking simulation	849:876	It was indicated by FTIR spectra that the components could interact with each other through hydrogen bonds, which was also confirmed by molecular docking simulation.					
37148940	9	6	theme	packaging	1351:1359	arg1	material					1361:1368	a novel bioactive packaging material	1333:1368	a novel bioactive packaging material with wide application potential in food preservation	1333:1421	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	9	6	theme	packaging	1351:1359	arg1	film					1319:1322	TP-SA-KGM film	1309:1322	TP-SA-KGM film	1309:1322	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	0	7	theme	food	96:99	arg1	preservation					101:112	food preservation	96:112	food preservation	96:112	Composite films of sodium alginate and konjac glucomannan incorporated with tea polyphenols for food preservation.					
37148940	9	8	theme	preservation	1242:1253	arg1	effects					1255:1261	excellent preservation effects	1232:1261	excellent preservation effects	1232:1261	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	7	9	theme	barrier	917:923	arg1	property					925:932	barrier property	917:932	barrier property	917:932	Meanwhile, the mechanical properties, barrier property, oxidation property, antibacterial activity, and stability of the structure of the TP-SA-KGM film were significantly improved.					
37148940	9	10	from	application	1380:1390	arg1	preservation					1410:1421	food preservation	1405:1421	food preservation	1405:1421	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	4	11	theme	tea	575:577	arg1	TP					592:593	TP	592:593	TP	592:593	A novel packaging film based on sodium alginate and konjac glucomannan (SA-KGM) blended with tea polyphenols (TP) was prepared in the present study.					
37148940	4	11	theme	tea	575:577	arg1	polyphenols					579:589	tea polyphenols	575:589	tea polyphenols (TP)	575:594	A novel packaging film based on sodium alginate and konjac glucomannan (SA-KGM) blended with tea polyphenols (TP) was prepared in the present study.					
37148940	8	12	theme	docking	1101:1107	arg1	simulation					1109:1118	molecular docking simulation	1091:1118	molecular docking simulation	1091:1118	The AFM images and results of molecular docking simulation indicated that TP could affect the cell wall of bacteria by acting with peptidoglycan.					
37148940	7	13	theme	mechanical	894:903	arg1	properties					905:914	the mechanical properties	890:914	the mechanical properties	890:914	Meanwhile, the mechanical properties, barrier property, oxidation property, antibacterial activity, and stability of the structure of the TP-SA-KGM film were significantly improved.					
37148940	9	14	theme	potential	1392:1400	arg1	application					1380:1390	wide application potential	1375:1400	wide application potential in food preservation	1375:1421	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	1	15	theme	serious	151:157	arg1	issue					159:163	a serious issue	149:163	a serious issue	149:163	At present, food waste has become a serious issue and the use of petroleum-based food packaging films has resulted in a series of potential hazards.					
37148940	0	16	theme	konjac	39:44	arg1	films					10:14	Composite films	0:14	Composite films of sodium alginate and konjac	0:44	Composite films of sodium alginate and konjac glucomannan incorporated with tea polyphenols for food preservation.					
37148940	4	17	theme	present	616:622	arg1	study					624:628	the present study	612:628	the present study	612:628	A novel packaging film based on sodium alginate and konjac glucomannan (SA-KGM) blended with tea polyphenols (TP) was prepared in the present study.					
37148940	8	18	theme	simulation	1109:1118	arg1	results					1080:1086	results	1080:1086	results	1080:1086	The AFM images and results of molecular docking simulation indicated that TP could affect the cell wall of bacteria by acting with peptidoglycan.					
37148940	8	18	theme	simulation	1109:1118	arg1	images					1069:1074	The AFM images	1061:1074	The AFM images	1061:1074	The AFM images and results of molecular docking simulation indicated that TP could affect the cell wall of bacteria by acting with peptidoglycan.					
37148940	7	19	theme	film	1027:1030	arg1	structure					1000:1008	the structure	996:1008	the structure of the TP-SA-KGM film	996:1030	Meanwhile, the mechanical properties, barrier property, oxidation property, antibacterial activity, and stability of the structure of the TP-SA-KGM film were significantly improved.					
37148940	6	20	theme	FTIR	733:736	arg1	spectra					738:744	FTIR spectra	733:744	FTIR spectra	733:744	It was indicated by FTIR spectra that the components could interact with each other through hydrogen bonds, which was also confirmed by molecular docking simulation.					
37148940	2	21	theme	materials	348:356	arg1	development					314:324	the development	310:324	the development of new food packaging materials	310:356	Therefore, more attention has been focused on the development of new food packaging materials.					
37148940	0	22	theme	Composite	0:8	arg1	films					10:14	Composite films	0:14	Composite films of sodium alginate and konjac	0:44	Composite films of sodium alginate and konjac glucomannan incorporated with tea polyphenols for food preservation.					
37148940	1	23	theme	potential	245:253	arg1	hazards					255:261	potential hazards	245:261	potential hazards	245:261	At present, food waste has become a serious issue and the use of petroleum-based food packaging films has resulted in a series of potential hazards.					
37148940	2	24	theme	packaging	338:346	arg1	materials					348:356	new food packaging materials	329:356	new food packaging materials	329:356	Therefore, more attention has been focused on the development of new food packaging materials.					
37148940	0	25	theme	sodium	19:24	arg1	alginate					26:33	sodium alginate	19:33	sodium alginate	19:33	Composite films of sodium alginate and konjac glucomannan incorporated with tea polyphenols for food preservation.					
37148940	1	26	theme	hazards	255:261	arg1	series					235:240	a series	233:240	a series of potential hazards	233:261	At present, food waste has become a serious issue and the use of petroleum-based food packaging films has resulted in a series of potential hazards.					
37148940	9	27	theme	wide	1375:1378	arg1	application					1380:1390	wide application potential	1375:1400	wide application potential in food preservation	1375:1421	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	9	28	theme	food	1405:1408	arg1	preservation					1410:1421	food preservation	1405:1421	food preservation	1405:1421	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	7	29	theme	TP-SA-KGM	1017:1025	arg1	film					1027:1030	the TP-SA-KGM film	1013:1030	the TP-SA-KGM film	1013:1030	Meanwhile, the mechanical properties, barrier property, oxidation property, antibacterial activity, and stability of the structure of the TP-SA-KGM film were significantly improved.					
37148940	6	30	theme	docking	859:865	arg1	simulation					867:876	molecular docking simulation	849:876	molecular docking simulation	849:876	It was indicated by FTIR spectra that the components could interact with each other through hydrogen bonds, which was also confirmed by molecular docking simulation.					
37148940	3	31	theme	excellent	449:457	arg1	film					394:397	The polysaccharide-based composite film	359:397	The polysaccharide-based composite film loaded with active substances	359:427	The polysaccharide-based composite film loaded with active substances considered to be an excellent preservative material.					
37148940	3	31	theme	excellent	449:457	arg1	material					472:479	an excellent preservative material	446:479	an excellent preservative material	446:479	The polysaccharide-based composite film loaded with active substances considered to be an excellent preservative material.					
37148940	7	32	theme	structure	1000:1008	arg1	property					945:952	oxidation property	935:952	oxidation property	935:952	Meanwhile, the mechanical properties, barrier property, oxidation property, antibacterial activity, and stability of the structure of the TP-SA-KGM film were significantly improved.					
37148940	7	32	theme	structure	1000:1008	arg1	stability					983:991	stability	983:991	stability	983:991	Meanwhile, the mechanical properties, barrier property, oxidation property, antibacterial activity, and stability of the structure of the TP-SA-KGM film were significantly improved.					
37148940	7	32	theme	structure	1000:1008	arg1	properties					905:914	the mechanical properties	890:914	the mechanical properties	890:914	Meanwhile, the mechanical properties, barrier property, oxidation property, antibacterial activity, and stability of the structure of the TP-SA-KGM film were significantly improved.					
37148940	7	32	theme	structure	1000:1008	arg1	activity					969:976	antibacterial activity	955:976	antibacterial activity	955:976	Meanwhile, the mechanical properties, barrier property, oxidation property, antibacterial activity, and stability of the structure of the TP-SA-KGM film were significantly improved.					
37148940	7	32	theme	structure	1000:1008	arg1	Meanwhile					879:887	Meanwhile	879:887	Meanwhile	879:887	Meanwhile, the mechanical properties, barrier property, oxidation property, antibacterial activity, and stability of the structure of the TP-SA-KGM film were significantly improved.					
37148940	7	32	theme	structure	1000:1008	arg1	property					925:932	barrier property	917:932	barrier property	917:932	Meanwhile, the mechanical properties, barrier property, oxidation property, antibacterial activity, and stability of the structure of the TP-SA-KGM film were significantly improved.					
37148940	0	33	dep	glucomannan	46:56	arg1	incorporated					58:69	incorporated	58:69	glucomannan incorporated with tea polyphenols for food preservation	46:112	Composite films of sodium alginate and konjac glucomannan incorporated with tea polyphenols for food preservation.					
37148940	3	34	theme	active	411:416	arg1	substances					418:427	active substances	411:427	active substances	411:427	The polysaccharide-based composite film loaded with active substances considered to be an excellent preservative material.					
37148940	1	35	theme	petroleum-based	180:194	arg1	films					211:215	petroleum-based food packaging films	180:215	petroleum-based food packaging films	180:215	At present, food waste has become a serious issue and the use of petroleum-based food packaging films has resulted in a series of potential hazards.					
37148940	0	36	theme	alginate	26:33	arg1	films					10:14	Composite films	0:14	Composite films of sodium alginate and konjac	0:44	Composite films of sodium alginate and konjac glucomannan incorporated with tea polyphenols for food preservation.					
37148940	4	37	theme	sodium	514:519	arg1	alginate					521:528	sodium alginate	514:528	sodium alginate	514:528	A novel packaging film based on sodium alginate and konjac glucomannan (SA-KGM) blended with tea polyphenols (TP) was prepared in the present study.					
37148940	4	37	theme	sodium	514:519	arg1	SA-KGM					554:559	SA-KGM	554:559	SA-KGM	554:559	A novel packaging film based on sodium alginate and konjac glucomannan (SA-KGM) blended with tea polyphenols (TP) was prepared in the present study.					
37148940	5	38	theme	atomic	682:687	arg1	AFM					707:709	AFM	707:709	AFM	707:709	The excellent microstructure of films was shown by atomic force microscopy (AFM).					
37148940	5	38	theme	atomic	682:687	arg1	microscopy					695:704	atomic force microscopy	682:704	atomic force microscopy (AFM)	682:710	The excellent microstructure of films was shown by atomic force microscopy (AFM).					
37148940	6	39	theme	hydrogen	805:812	arg1	bonds					814:818	hydrogen bonds	805:818	hydrogen bonds	805:818	It was indicated by FTIR spectra that the components could interact with each other through hydrogen bonds, which was also confirmed by molecular docking simulation.					
37148940	9	40	with	material	1361:1368	arg1	application					1380:1390	wide application potential	1375:1400	wide application potential in food preservation	1375:1421	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	1	41	theme	food	196:199	arg1	films					211:215	petroleum-based food packaging films	180:215	petroleum-based food packaging films	180:215	At present, food waste has become a serious issue and the use of petroleum-based food packaging films has resulted in a series of potential hazards.					
37148940	3	42	theme	polysaccharide-based	363:382	arg1	film					394:397	The polysaccharide-based composite film	359:397	The polysaccharide-based composite film loaded with active substances	359:427	The polysaccharide-based composite film loaded with active substances considered to be an excellent preservative material.					
37148940	3	42	theme	polysaccharide-based	363:382	arg1	material					472:479	an excellent preservative material	446:479	an excellent preservative material	446:479	The polysaccharide-based composite film loaded with active substances considered to be an excellent preservative material.					
37148940	5	43	theme	force	689:693	arg1	AFM					707:709	AFM	707:709	AFM	707:709	The excellent microstructure of films was shown by atomic force microscopy (AFM).					
37148940	5	43	theme	force	689:693	arg1	microscopy					695:704	atomic force microscopy	682:704	atomic force microscopy (AFM)	682:710	The excellent microstructure of films was shown by atomic force microscopy (AFM).					
37148940	5	44	theme	excellent	635:643	arg1	microstructure					645:658	The excellent microstructure	631:658	The excellent microstructure of films	631:667	The excellent microstructure of films was shown by atomic force microscopy (AFM).					
37148940	4	45	theme	konjac	534:539	arg1	glucomannan					541:551	konjac glucomannan	534:551	konjac glucomannan	534:551	A novel packaging film based on sodium alginate and konjac glucomannan (SA-KGM) blended with tea polyphenols (TP) was prepared in the present study.					
37148940	3	46	theme	composite	384:392	arg1	film					394:397	The polysaccharide-based composite film	359:397	The polysaccharide-based composite film loaded with active substances	359:427	The polysaccharide-based composite film loaded with active substances considered to be an excellent preservative material.					
37148940	3	46	theme	composite	384:392	arg1	material					472:479	an excellent preservative material	446:479	an excellent preservative material	446:479	The polysaccharide-based composite film loaded with active substances considered to be an excellent preservative material.					
37148940	9	47	theme	TP-SA-KGM	1309:1317	arg1	material					1361:1368	a novel bioactive packaging material	1333:1368	a novel bioactive packaging material with wide application potential in food preservation	1333:1421	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	9	47	theme	TP-SA-KGM	1309:1317	arg1	film					1319:1322	TP-SA-KGM film	1309:1322	TP-SA-KGM film	1309:1322	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	8	48	theme	cell	1155:1158	arg1	wall					1160:1163	the cell wall	1151:1163	the cell wall of bacteria	1151:1175	The AFM images and results of molecular docking simulation indicated that TP could affect the cell wall of bacteria by acting with peptidoglycan.					
37148940	7	49	theme	antibacterial	955:967	arg1	activity					969:976	antibacterial activity	955:976	antibacterial activity	955:976	Meanwhile, the mechanical properties, barrier property, oxidation property, antibacterial activity, and stability of the structure of the TP-SA-KGM film were significantly improved.					
37148940	3	50	theme	preservative	459:470	arg1	film					394:397	The polysaccharide-based composite film	359:397	The polysaccharide-based composite film loaded with active substances	359:427	The polysaccharide-based composite film loaded with active substances considered to be an excellent preservative material.					
37148940	3	50	theme	preservative	459:470	arg1	material					472:479	an excellent preservative material	446:479	an excellent preservative material	446:479	The polysaccharide-based composite film loaded with active substances considered to be an excellent preservative material.					
37148940	1	51	theme	packaging	201:209	arg1	films					211:215	petroleum-based food packaging films	180:215	petroleum-based food packaging films	180:215	At present, food waste has become a serious issue and the use of petroleum-based food packaging films has resulted in a series of potential hazards.					
37148940	5	52	theme	films	663:667	arg1	microstructure					645:658	The excellent microstructure	631:658	The excellent microstructure of films	631:667	The excellent microstructure of films was shown by atomic force microscopy (AFM).					
37148940	7	53	theme	oxidation	935:943	arg1	property					945:952	oxidation property	935:952	oxidation property	935:952	Meanwhile, the mechanical properties, barrier property, oxidation property, antibacterial activity, and stability of the structure of the TP-SA-KGM film were significantly improved.					
37148940	1	54	theme	food	127:130	arg1	waste					132:136	food waste	127:136	food waste	127:136	At present, food waste has become a serious issue and the use of petroleum-based food packaging films has resulted in a series of potential hazards.					
37148940	1	55	theme	films	211:215	arg1	use					173:175	the use	169:175	the use of petroleum-based food packaging films	169:215	At present, food waste has become a serious issue and the use of petroleum-based food packaging films has resulted in a series of potential hazards.					
37148940	2	56	theme	food	333:336	arg1	materials					348:356	new food packaging materials	329:356	new food packaging materials	329:356	Therefore, more attention has been focused on the development of new food packaging materials.					
37148940	9	57	theme	excellent	1232:1240	arg1	effects					1255:1261	excellent preservation effects	1232:1261	excellent preservation effects	1232:1261	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	8	58	theme	bacteria	1168:1175	arg1	wall					1160:1163	the cell wall	1151:1163	the cell wall of bacteria	1151:1175	The AFM images and results of molecular docking simulation indicated that TP could affect the cell wall of bacteria by acting with peptidoglycan.					
37148940	4	59	theme	packaging	490:498	arg1	film					500:503	A novel packaging film	482:503	A novel packaging film based on sodium alginate and konjac glucomannan (SA-KGM) blended with tea polyphenols (TP)	482:594	A novel packaging film based on sodium alginate and konjac glucomannan (SA-KGM) blended with tea polyphenols (TP) was prepared in the present study.					
37148940	2	60	theme	new	329:331	arg1	materials					348:356	new food packaging materials	329:356	new food packaging materials	329:356	Therefore, more attention has been focused on the development of new food packaging materials.					
37148940	9	61	theme	novel	1335:1339	arg1	material					1361:1368	a novel bioactive packaging material	1333:1368	a novel bioactive packaging material with wide application potential in food preservation	1333:1421	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37148940	9	61	theme	novel	1335:1339	arg1	film					1319:1322	TP-SA-KGM film	1309:1322	TP-SA-KGM film	1309:1322	Finally, the film showed excellent preservation effects in both beef and apples, which suggested that TP-SA-KGM film could be a novel bioactive packaging material with wide application potential in food preservation.					
37059019	0	0	theme	heavy	92:96	arg1	metals					98:103	trace heavy metals	86:103	trace heavy metals	86:103	Electrospun composite nanofibers modified with silver nanoparticles for extraction of trace heavy metals from water and rice samples: An highly efficient and reproducible sorbent.					
37059019	2	1	theme	AgNPs	611:615	arg1	dispersion					597:606	highly uniform dispersion	582:606	highly uniform dispersion of AgNPs in the nanofibers	582:633	Incorporating agar into the nanofibers followed by in situ photo-reductive reaction under UV-lamp resulted in highly uniform dispersion of AgNPs in the nanofibers.					
37059019	5	2	dep	%	893:893	arg1	intra-day					896:904	intra-day	896:904	intra-day	896:904	The relative standard deviations (RSDs) were between 4.5% and 5.6% (intra-day, n = 5) and 5.3%-5.9% (inter-day, n = 3) for three sequential days.					
37059019	5	2	dep	%	893:893	arg1	%					926:926	-5.9%	922:926	-5.9%	922:926	The relative standard deviations (RSDs) were between 4.5% and 5.6% (intra-day, n = 5) and 5.3%-5.9% (inter-day, n = 3) for three sequential days.					
37059019	5	2	dep	%	893:893	arg1	n = 3					940:944	n = 3	940:944	n = 3	940:944	The relative standard deviations (RSDs) were between 4.5% and 5.6% (intra-day, n = 5) and 5.3%-5.9% (inter-day, n = 3) for three sequential days.					
37059019	5	2	dep	%	893:893	arg1	n = 5					907:911	n = 5	907:911	n = 5	907:911	The relative standard deviations (RSDs) were between 4.5% and 5.6% (intra-day, n = 5) and 5.3%-5.9% (inter-day, n = 3) for three sequential days.					
37059019	5	2	dep	%	893:893	arg1	inter-day					929:937	inter-day	929:937	inter-day	929:937	The relative standard deviations (RSDs) were between 4.5% and 5.6% (intra-day, n = 5) and 5.3%-5.9% (inter-day, n = 3) for three sequential days.					
37059019	1	3	theme	nanoparticles	239:251	arg1	nanofibers					273:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	0	4	theme	trace	86:90	arg1	metals					98:103	trace heavy metals	86:103	trace heavy metals	86:103	Electrospun composite nanofibers modified with silver nanoparticles for extraction of trace heavy metals from water and rice samples: An highly efficient and reproducible sorbent.					
37059019	1	5	theme	plasma	424:429	arg1	ICP-OES					462:468	ICP-OES	462:468	ICP-OES	462:468	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	5	theme	plasma	424:429	arg1	spectroscopy					448:459	plasma optical emission spectroscopy	424:459	inductively coupled plasma optical emission spectroscopy (ICP-OES)	404:469	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	6	6	theme	metal	1157:1161	arg1	ions					1163:1166	heavy metal ions	1151:1166	heavy metal ions in varied samples	1151:1184	The developed method was investigated with water and rice samples, and recoveries (93.9-98.0%) indicated that the PAN/agar/AgNPs could be a promising film for the adsorption of heavy metal ions in varied samples.					
37059019	6	7	theme	varied	1171:1176	arg1	samples					1178:1184	varied samples	1171:1184	varied samples	1171:1184	The developed method was investigated with water and rice samples, and recoveries (93.9-98.0%) indicated that the PAN/agar/AgNPs could be a promising film for the adsorption of heavy metal ions in varied samples.					
37059019	2	8	theme	in	523:524	arg1	reaction					547:554	in situ photo-reductive reaction	523:554	in situ photo-reductive reaction	523:554	Incorporating agar into the nanofibers followed by in situ photo-reductive reaction under UV-lamp resulted in highly uniform dispersion of AgNPs in the nanofibers.					
37059019	1	9	theme	thin-film	339:347	arg1	TFME					367:370	TFME	367:370	TFME	367:370	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	9	theme	thin-film	339:347	arg1	micro-extraction					349:364	thin-film micro-extraction	339:364	thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES)	339:469	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	6	10	theme	ions	1163:1166	arg1	adsorption					1137:1146	the adsorption	1133:1146	the adsorption of heavy metal ions in varied samples	1133:1184	The developed method was investigated with water and rice samples, and recoveries (93.9-98.0%) indicated that the PAN/agar/AgNPs could be a promising film for the adsorption of heavy metal ions in varied samples.					
37059019	1	11	theme	optical	431:437	arg1	ICP-OES					462:468	ICP-OES	462:468	ICP-OES	462:468	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	11	theme	optical	431:437	arg1	spectroscopy					448:459	plasma optical emission spectroscopy	424:459	inductively coupled plasma optical emission spectroscopy (ICP-OES)	404:469	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	12	theme	AgNPs	254:258	arg1	nanofibers					273:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	0	13	theme	metals	98:103	arg1	extraction					72:81	extraction	72:81	extraction of trace heavy metals from water and rice samples	72:131	Electrospun composite nanofibers modified with silver nanoparticles for extraction of trace heavy metals from water and rice samples: An highly efficient and reproducible sorbent.					
37059019	2	14	theme	uniform	589:595	arg1	dispersion					597:606	highly uniform dispersion	582:606	highly uniform dispersion of AgNPs in the nanofibers	582:633	Incorporating agar into the nanofibers followed by in situ photo-reductive reaction under UV-lamp resulted in highly uniform dispersion of AgNPs in the nanofibers.					
37059019	1	15	theme	emission	439:446	arg1	ICP-OES					462:468	ICP-OES	462:468	ICP-OES	462:468	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	15	theme	emission	439:446	arg1	spectroscopy					448:459	plasma optical emission spectroscopy	424:459	inductively coupled plasma optical emission spectroscopy (ICP-OES)	404:469	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	4	16	theme	0.5 ng mL-1	815:825	arg1	range					799:803	the range	795:803	the range of 0.2 to 0.5 ng mL-1	795:825	The LODs (based on S/N = 3) were attained in the range of 0.2 to 0.5 ng mL-1.					
37059019	5	17	theme	relative	832:839	arg1	RSDs					862:865	RSDs	862:865	RSDs	862:865	The relative standard deviations (RSDs) were between 4.5% and 5.6% (intra-day, n = 5) and 5.3%-5.9% (inter-day, n = 3) for three sequential days.					
37059019	5	17	theme	relative	832:839	arg1	deviations					850:859	The relative standard deviations	828:859	The relative standard deviations (RSDs)	828:866	The relative standard deviations (RSDs) were between 4.5% and 5.6% (intra-day, n = 5) and 5.3%-5.9% (inter-day, n = 3) for three sequential days.					
37059019	0	18	theme	water	110:114	arg1	samples					125:131	water and rice samples	110:131	samples	125:131	Electrospun composite nanofibers modified with silver nanoparticles for extraction of trace heavy metals from water and rice samples: An highly efficient and reproducible sorbent.					
37059019	6	19	from	adsorption	1137:1146	arg1	samples					1178:1184	varied samples	1171:1184	varied samples	1171:1184	The developed method was investigated with water and rice samples, and recoveries (93.9-98.0%) indicated that the PAN/agar/AgNPs could be a promising film for the adsorption of heavy metal ions in varied samples.					
37059019	5	20	theme	standard	841:848	arg1	RSDs					862:865	RSDs	862:865	RSDs	862:865	The relative standard deviations (RSDs) were between 4.5% and 5.6% (intra-day, n = 5) and 5.3%-5.9% (inter-day, n = 3) for three sequential days.					
37059019	5	20	theme	standard	841:848	arg1	deviations					850:859	The relative standard deviations	828:859	The relative standard deviations (RSDs)	828:866	The relative standard deviations (RSDs) were between 4.5% and 5.6% (intra-day, n = 5) and 5.3%-5.9% (inter-day, n = 3) for three sequential days.					
37059019	0	21	theme	composite	12:20	arg1	nanofibers					22:31	Electrospun composite nanofibers	0:31	Electrospun composite nanofibers	0:31	Electrospun composite nanofibers modified with silver nanoparticles for extraction of trace heavy metals from water and rice samples: An highly efficient and reproducible sorbent.					
37059019	3	22	theme	0.5-250.0 ng mL-1	717:733	arg1	range					708:712	the range	704:712	the range of 0.5-250.0 ng mL-1 (R2 ≥ 0.9985)	704:747	Under the optimized conditions, agreeable linearity was acquired in the range of 0.5-250.0 ng mL-1 (R2 ≥ 0.9985).					
37059019	6	23	dep	water	1017:1021	arg1	samples					1032:1038	samples	1032:1038	samples	1032:1038	The developed method was investigated with water and rice samples, and recoveries (93.9-98.0%) indicated that the PAN/agar/AgNPs could be a promising film for the adsorption of heavy metal ions in varied samples.					
37059019	2	24	from	dispersion	597:606	arg1	nanofibers					624:633	the nanofibers	620:633	the nanofibers	620:633	Incorporating agar into the nanofibers followed by in situ photo-reductive reaction under UV-lamp resulted in highly uniform dispersion of AgNPs in the nanofibers.					
37059019	0	25	theme	Electrospun	0:10	arg1	nanofibers					22:31	Electrospun composite nanofibers	0:31	Electrospun composite nanofibers	0:31	Electrospun composite nanofibers modified with silver nanoparticles for extraction of trace heavy metals from water and rice samples: An highly efficient and reproducible sorbent.					
37059019	1	26	theme	electrospun	261:271	arg1	nanofibers					273:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	3	27	theme	agreeable	668:676	arg1	linearity					678:686	agreeable linearity	668:686	agreeable linearity	668:686	Under the optimized conditions, agreeable linearity was acquired in the range of 0.5-250.0 ng mL-1 (R2 ≥ 0.9985).					
37059019	6	28	theme	heavy	1151:1155	arg1	ions					1163:1166	heavy metal ions	1151:1166	heavy metal ions in varied samples	1151:1184	The developed method was investigated with water and rice samples, and recoveries (93.9-98.0%) indicated that the PAN/agar/AgNPs could be a promising film for the adsorption of heavy metal ions in varied samples.					
37059019	4	29	dep	0.5 ng mL-1	815:825	arg1	to					812:813	to	812:813	to	812:813	The LODs (based on S/N = 3) were attained in the range of 0.2 to 0.5 ng mL-1.					
37059019	1	30	theme	nanofibers	273:282	arg1	composite					190:198	a composite	188:198	a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	188:282	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	30	theme	nanofibers	273:282	arg1	sorbent					327:333	an efficient sorbent	314:333	an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES)	314:469	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	30	theme	nanofibers	273:282	arg1	nanofibers					273:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	2	31	from	AgNPs	611:615	arg1	nanofibers					624:633	the nanofibers	620:633	the nanofibers	620:633	Incorporating agar into the nanofibers followed by in situ photo-reductive reaction under UV-lamp resulted in highly uniform dispersion of AgNPs in the nanofibers.					
37059019	0	32	theme	rice	120:123	arg1	samples					125:131	water and rice samples	110:131	samples	125:131	Electrospun composite nanofibers modified with silver nanoparticles for extraction of trace heavy metals from water and rice samples: An highly efficient and reproducible sorbent.					
37059019	3	33	theme	optimized	646:654	arg1	conditions					656:665	the optimized conditions	642:665	the optimized conditions	642:665	Under the optimized conditions, agreeable linearity was acquired in the range of 0.5-250.0 ng mL-1 (R2 ≥ 0.9985).					
37059019	0	34	theme	silver	47:52	arg1	nanoparticles					54:66	silver nanoparticles	47:66	silver nanoparticles for extraction of trace heavy metals from water and rice samples	47:131	Electrospun composite nanofibers modified with silver nanoparticles for extraction of trace heavy metals from water and rice samples: An highly efficient and reproducible sorbent.					
37059019	2	35	theme	photo-reductive	531:545	arg1	reaction					547:554	in situ photo-reductive reaction	523:554	in situ photo-reductive reaction	523:554	Incorporating agar into the nanofibers followed by in situ photo-reductive reaction under UV-lamp resulted in highly uniform dispersion of AgNPs in the nanofibers.					
37059019	1	36	theme	metal	381:385	arg1	ions					387:390	five metal ions	376:390	five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES)	376:469	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	37	theme	polyacrylonitrile	203:219	arg1	nanofibers					273:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	2	38	dep	in	523:524	arg1	situ					526:529	situ	526:529	situ	526:529	Incorporating agar into the nanofibers followed by in situ photo-reductive reaction under UV-lamp resulted in highly uniform dispersion of AgNPs in the nanofibers.					
37059019	6	39	from	samples	1178:1184	arg1	adsorption					1137:1146	the adsorption	1133:1146	the adsorption of heavy metal ions in varied samples	1133:1184	The developed method was investigated with water and rice samples, and recoveries (93.9-98.0%) indicated that the PAN/agar/AgNPs could be a promising film for the adsorption of heavy metal ions in varied samples.					
37059019	0	40	theme	efficient	144:152	arg1	sorbent					171:177	An highly efficient and reproducible sorbent	134:177	An highly efficient and reproducible sorbent	134:177	Electrospun composite nanofibers modified with silver nanoparticles for extraction of trace heavy metals from water and rice samples: An highly efficient and reproducible sorbent.					
37059019	1	41	theme	ions	387:390	arg1	TFME					367:370	TFME	367:370	TFME	367:370	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	41	theme	ions	387:390	arg1	micro-extraction					349:364	thin-film micro-extraction	339:364	thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES)	339:469	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	6	42	theme	promising	1114:1122	arg1	film					1124:1127	a promising film	1112:1127	a promising film for the adsorption of heavy metal ions in varied samples	1112:1184	The developed method was investigated with water and rice samples, and recoveries (93.9-98.0%) indicated that the PAN/agar/AgNPs could be a promising film for the adsorption of heavy metal ions in varied samples.					
37059019	6	42	theme	promising	1114:1122	arg1	PAN/agar/AgNPs					1088:1101	the PAN/agar/AgNPs	1084:1101	the PAN/agar/AgNPs	1084:1101	The developed method was investigated with water and rice samples, and recoveries (93.9-98.0%) indicated that the PAN/agar/AgNPs could be a promising film for the adsorption of heavy metal ions in varied samples.					
37059019	2	43	from	nanofibers	624:633	arg1	dispersion					597:606	highly uniform dispersion	582:606	highly uniform dispersion of AgNPs in the nanofibers	582:633	Incorporating agar into the nanofibers followed by in situ photo-reductive reaction under UV-lamp resulted in highly uniform dispersion of AgNPs in the nanofibers.					
37059019	0	44	theme	reproducible	158:169	arg1	sorbent					171:177	An highly efficient and reproducible sorbent	134:177	An highly efficient and reproducible sorbent	134:177	Electrospun composite nanofibers modified with silver nanoparticles for extraction of trace heavy metals from water and rice samples: An highly efficient and reproducible sorbent.					
37059019	0	45	dep	modified	33:40	arg1	sorbent					171:177	An highly efficient and reproducible sorbent	134:177	An highly efficient and reproducible sorbent	134:177	Electrospun composite nanofibers modified with silver nanoparticles for extraction of trace heavy metals from water and rice samples: An highly efficient and reproducible sorbent.					
37059019	6	46	from	ions	1163:1166	arg1	samples					1178:1184	varied samples	1171:1184	varied samples	1171:1184	The developed method was investigated with water and rice samples, and recoveries (93.9-98.0%) indicated that the PAN/agar/AgNPs could be a promising film for the adsorption of heavy metal ions in varied samples.					
37059019	5	47	theme	sequential	957:966	arg1	days					968:971	three sequential days	951:971	three sequential days	951:971	The relative standard deviations (RSDs) were between 4.5% and 5.6% (intra-day, n = 5) and 5.3%-5.9% (inter-day, n = 3) for three sequential days.					
37059019	1	48	theme	PAN	222:224	arg1	nanofibers					273:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	49	theme	coupled	416:422	arg1	ICP-OES					462:468	ICP-OES	462:468	ICP-OES	462:468	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	49	theme	coupled	416:422	arg1	spectroscopy					448:459	plasma optical emission spectroscopy	424:459	inductively coupled plasma optical emission spectroscopy (ICP-OES)	404:469	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	6	50	theme	developed	978:986	arg1	method					988:993	The developed method	974:993	The developed method	974:993	The developed method was investigated with water and rice samples, and recoveries (93.9-98.0%) indicated that the PAN/agar/AgNPs could be a promising film for the adsorption of heavy metal ions in varied samples.					
37059019	0	51	from	samples	125:131	arg1	extraction					72:81	extraction	72:81	extraction of trace heavy metals from water and rice samples	72:131	Electrospun composite nanofibers modified with silver nanoparticles for extraction of trace heavy metals from water and rice samples: An highly efficient and reproducible sorbent.					
37059019	1	52	theme	efficient	317:325	arg1	composite					190:198	a composite	188:198	a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	188:282	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	52	theme	efficient	317:325	arg1	sorbent					327:333	an efficient sorbent	314:333	an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES)	314:469	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	52	theme	efficient	317:325	arg1	nanofibers					273:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
37059019	1	53	theme	/agar/silver	226:237	arg1	nanofibers					273:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers	203:282	Herein, a composite of polyacrylonitrile (PAN)/agar/silver nanoparticles (AgNPs) electrospun nanofibers was fabricated and applied as an efficient sorbent for thin-film micro-extraction (TFME) of five metal ions followed by inductively coupled plasma optical emission spectroscopy (ICP-OES).					
35725198	5	0	theme	bone-related	969:980	arg1	markers					982:988	bone-related markers	969:988	bone-related markers (up to 2-fold)	969:1003	Furthermore, they increased alkaline phosphatase activity (up to 10-fold) and up-regulated the expression of bone-related markers (up to 2-fold), without osteogenic supplementation, demonstrating their osteoinductive properties.					
35725198	4	1	theme	nanocomposite	662:674	arg1	scaffolds					676:684	The nanocomposite scaffolds	658:684	The nanocomposite scaffolds	658:684	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	5	2	theme	osteogenic	1014:1023	arg1	supplementation					1025:1039	osteogenic supplementation	1014:1039	osteogenic supplementation	1014:1039	Furthermore, they increased alkaline phosphatase activity (up to 10-fold) and up-regulated the expression of bone-related markers (up to 2-fold), without osteogenic supplementation, demonstrating their osteoinductive properties.					
35725198	4	3	theme	strain	756:761	arg1	cycles					763:768	75% strain cycles	752:768	75% strain cycles	752:768	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	0	4	theme	cellulose	99:107	arg1	nanocrystals					109:120	biomineralized cellulose nanocrystals	84:120	biomineralized cellulose nanocrystals	84:120	Highly elastic and bioactive bone biomimetic scaffolds based on platelet lysate and biomineralized cellulose nanocrystals.					
35725198	5	5	dep	increased	878:886	arg1	10-fold					925:931	10-fold	925:931	10-fold	925:931	Furthermore, they increased alkaline phosphatase activity (up to 10-fold) and up-regulated the expression of bone-related markers (up to 2-fold), without osteogenic supplementation, demonstrating their osteoinductive properties.					
35725198	3	6	theme	bioactivity	601:611	arg1	advantage					584:592	advantage	584:592	advantage of the bioactivity steaming from platelet-derived biomolecules	584:655	These mineralized-CNCs were combined with platelet lysate to produce nanocomposite scaffolds through cryogelation to mimic bone ECM protein-mineral composite nature and take advantage of the bioactivity steaming from platelet-derived biomolecules.					
35725198	4	7	link	platelet-derived	801:816	arg1	sequestration					833:845	sequestration	833:845	sequestration	833:845	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	4	7	link	platelet-derived	801:816	arg1	factors					825:831	modulated platelet-derived growth factors	791:831	modulated platelet-derived growth factors sequestration and release	791:857	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	4	7	link	platelet-derived	801:816	arg1	release					851:857	release	851:857	release	851:857	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	0	8	theme	biomineralized	84:97	arg1	nanocrystals					109:120	biomineralized cellulose nanocrystals	84:120	biomineralized cellulose nanocrystals	84:120	Highly elastic and bioactive bone biomimetic scaffolds based on platelet lysate and biomineralized cellulose nanocrystals.					
35725198	5	9	dep	2-fold	997:1002	arg1	up					991:992	up	991:992	up	991:992	Furthermore, they increased alkaline phosphatase activity (up to 10-fold) and up-regulated the expression of bone-related markers (up to 2-fold), without osteogenic supplementation, demonstrating their osteoinductive properties.					
35725198	2	10	theme	phosphates	293:302	arg1	nucleation					271:280	the nucleation	267:280	the nucleation of calcium phosphates during the biomineralization process	267:339	Herein, the nucleation of calcium phosphates during the biomineralization process was mimicked using negatively-charged cellulose nanocrystals (CNCs).					
35725198	3	11	theme	ECM	538:540	arg1	nature					568:573	bone ECM protein-mineral composite nature	533:573	bone ECM protein-mineral composite nature	533:573	These mineralized-CNCs were combined with platelet lysate to produce nanocomposite scaffolds through cryogelation to mimic bone ECM protein-mineral composite nature and take advantage of the bioactivity steaming from platelet-derived biomolecules.					
35725198	3	12	theme	protein-mineral	542:556	arg1	nature					568:573	bone ECM protein-mineral composite nature	533:573	bone ECM protein-mineral composite nature	533:573	These mineralized-CNCs were combined with platelet lysate to produce nanocomposite scaffolds through cryogelation to mimic bone ECM protein-mineral composite nature and take advantage of the bioactivity steaming from platelet-derived biomolecules.					
35725198	2	13	theme	calcium	285:291	arg1	phosphates					293:302	calcium phosphates	285:302	calcium phosphates	285:302	Herein, the nucleation of calcium phosphates during the biomineralization process was mimicked using negatively-charged cellulose nanocrystals (CNCs).					
35725198	3	14	theme	nanocomposite	479:491	arg1	scaffolds					493:501	nanocomposite scaffolds	479:501	nanocomposite scaffolds	479:501	These mineralized-CNCs were combined with platelet lysate to produce nanocomposite scaffolds through cryogelation to mimic bone ECM protein-mineral composite nature and take advantage of the bioactivity steaming from platelet-derived biomolecules.					
35725198	5	15	theme	markers	982:988	arg1	expression					955:964	the expression	951:964	the expression of bone-related markers (up to 2-fold)	951:1003	Furthermore, they increased alkaline phosphatase activity (up to 10-fold) and up-regulated the expression of bone-related markers (up to 2-fold), without osteogenic supplementation, demonstrating their osteoinductive properties.					
35725198	0	16	theme	biomimetic	34:43	arg1	scaffolds					45:53	Highly elastic and bioactive bone biomimetic scaffolds	0:53	Highly elastic and bioactive bone biomimetic scaffolds	0:53	Highly elastic and bioactive bone biomimetic scaffolds based on platelet lysate and biomineralized cellulose nanocrystals.					
35725198	7	17	theme	nanocomposite	1282:1294	arg1	scaffolds					1296:1304	the mineralized nanocomposite scaffolds	1266:1304	the mineralized nanocomposite scaffolds	1266:1304	These results suggest that the mineralized nanocomposite scaffolds can enhance bone regeneration by simultaneously promoting osteogenesis and angiogenesis.					
35725198	0	18	theme	elastic	7:13	arg1	scaffolds					45:53	Highly elastic and bioactive bone biomimetic scaffolds	0:53	Highly elastic and bioactive bone biomimetic scaffolds	0:53	Highly elastic and bioactive bone biomimetic scaffolds based on platelet lysate and biomineralized cellulose nanocrystals.					
35725198	4	19	theme	modulated	791:799	arg1	sequestration					833:845	sequestration	833:845	sequestration	833:845	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	4	19	theme	modulated	791:799	arg1	factors					825:831	modulated platelet-derived growth factors	791:831	modulated platelet-derived growth factors sequestration and release	791:857	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	4	19	theme	modulated	791:799	arg1	release					851:857	release	851:857	release	851:857	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	7	20	theme	mineralized	1270:1280	arg1	scaffolds					1296:1304	the mineralized nanocomposite scaffolds	1266:1304	the mineralized nanocomposite scaffolds	1266:1304	These results suggest that the mineralized nanocomposite scaffolds can enhance bone regeneration by simultaneously promoting osteogenesis and angiogenesis.					
35725198	1	21	theme	heavily-mineralized	194:212	arg1	ECM					236:238	ECM	236:238	ECM	236:238	Bone is a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale.					
35725198	1	21	theme	heavily-mineralized	194:212	arg1	matrix					228:233	a heavily-mineralized extracellular matrix	192:233	a heavily-mineralized extracellular matrix (ECM)	192:239	Bone is a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale.					
35725198	5	22	theme	alkaline	888:895	arg1	phosphatase					897:907	alkaline phosphatase	888:907	alkaline phosphatase activity	888:916	Furthermore, they increased alkaline phosphatase activity (up to 10-fold) and up-regulated the expression of bone-related markers (up to 2-fold), without osteogenic supplementation, demonstrating their osteoinductive properties.					
35725198	1	23	theme	extracellular	214:226	arg1	ECM					236:238	ECM	236:238	ECM	236:238	Bone is a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale.					
35725198	1	23	theme	extracellular	214:226	arg1	matrix					228:233	a heavily-mineralized extracellular matrix	192:233	a heavily-mineralized extracellular matrix (ECM)	192:239	Bone is a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale.					
35725198	0	24	theme	bioactive	19:27	arg1	scaffolds					45:53	Highly elastic and bioactive bone biomimetic scaffolds	0:53	Highly elastic and bioactive bone biomimetic scaffolds	0:53	Highly elastic and bioactive bone biomimetic scaffolds based on platelet lysate and biomineralized cellulose nanocrystals.					
35725198	2	25	theme	biomineralization	315:331	arg1	process					333:339	the biomineralization process	311:339	the biomineralization process	311:339	Herein, the nucleation of calcium phosphates during the biomineralization process was mimicked using negatively-charged cellulose nanocrystals (CNCs).					
35725198	6	26	theme	endothelial	1136:1146	arg1	cells					1148:1152	endothelial cells	1136:1152	endothelial cells	1136:1152	Also, the scaffolds promoted the chemotaxis of endothelial cells and enhanced the expression of endothelial markers, showing proangiogenic potential.					
35725198	3	27	theme	platelet-derived	627:642	arg1	biomolecules					644:655	platelet-derived biomolecules	627:655	platelet-derived biomolecules	627:655	These mineralized-CNCs were combined with platelet lysate to produce nanocomposite scaffolds through cryogelation to mimic bone ECM protein-mineral composite nature and take advantage of the bioactivity steaming from platelet-derived biomolecules.					
35725198	4	28	theme	%	754:754	arg1	cycles					763:768	75% strain cycles	752:768	75% strain cycles	752:768	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	3	29	theme	platelet	452:459	arg1	lysate					461:466	platelet lysate	452:466	platelet lysate	452:466	These mineralized-CNCs were combined with platelet lysate to produce nanocomposite scaffolds through cryogelation to mimic bone ECM protein-mineral composite nature and take advantage of the bioactivity steaming from platelet-derived biomolecules.					
35725198	3	30	theme	composite	558:566	arg1	nature					568:573	bone ECM protein-mineral composite nature	533:573	bone ECM protein-mineral composite nature	533:573	These mineralized-CNCs were combined with platelet lysate to produce nanocomposite scaffolds through cryogelation to mimic bone ECM protein-mineral composite nature and take advantage of the bioactivity steaming from platelet-derived biomolecules.					
35725198	6	31	theme	proangiogenic	1214:1226	arg1	potential					1228:1236	proangiogenic potential	1214:1236	proangiogenic potential	1214:1236	Also, the scaffolds promoted the chemotaxis of endothelial cells and enhanced the expression of endothelial markers, showing proangiogenic potential.					
35725198	6	32	theme	cells	1148:1152	arg1	chemotaxis					1122:1131	the chemotaxis	1118:1131	the chemotaxis of endothelial cells	1118:1152	Also, the scaffolds promoted the chemotaxis of endothelial cells and enhanced the expression of endothelial markers, showing proangiogenic potential.					
35725198	4	33	theme	high	722:725	arg1	elasticity					727:736	high elasticity	722:736	high elasticity (recover from 75% strain cycles)	722:769	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	0	34	theme	bone	29:32	arg1	scaffolds					45:53	Highly elastic and bioactive bone biomimetic scaffolds	0:53	Highly elastic and bioactive bone biomimetic scaffolds	0:53	Highly elastic and bioactive bone biomimetic scaffolds based on platelet lysate and biomineralized cellulose nanocrystals.					
35725198	5	35	theme	phosphatase	897:907	arg1	activity					909:916	alkaline phosphatase activity	888:916	alkaline phosphatase activity	888:916	Furthermore, they increased alkaline phosphatase activity (up to 10-fold) and up-regulated the expression of bone-related markers (up to 2-fold), without osteogenic supplementation, demonstrating their osteoinductive properties.					
35725198	6	36	theme	markers	1197:1203	arg1	expression					1171:1180	the expression	1167:1180	the expression of endothelial markers	1167:1203	Also, the scaffolds promoted the chemotaxis of endothelial cells and enhanced the expression of endothelial markers, showing proangiogenic potential.					
35725198	2	37	theme	cellulose	379:387	arg1	CNCs					403:406	CNCs	403:406	CNCs	403:406	Herein, the nucleation of calcium phosphates during the biomineralization process was mimicked using negatively-charged cellulose nanocrystals (CNCs).					
35725198	2	37	theme	cellulose	379:387	arg1	nanocrystals					389:400	negatively-charged cellulose nanocrystals	360:400	negatively-charged cellulose nanocrystals (CNCs)	360:407	Herein, the nucleation of calcium phosphates during the biomineralization process was mimicked using negatively-charged cellulose nanocrystals (CNCs).					
35725198	2	38	theme	negatively-charged	360:377	arg1	CNCs					403:406	CNCs	403:406	CNCs	403:406	Herein, the nucleation of calcium phosphates during the biomineralization process was mimicked using negatively-charged cellulose nanocrystals (CNCs).					
35725198	2	38	theme	negatively-charged	360:377	arg1	nanocrystals					389:400	negatively-charged cellulose nanocrystals	360:400	negatively-charged cellulose nanocrystals (CNCs)	360:407	Herein, the nucleation of calcium phosphates during the biomineralization process was mimicked using negatively-charged cellulose nanocrystals (CNCs).					
35725198	4	39	dep	factors	825:831	arg1	sequestration					833:845	sequestration	833:845	sequestration	833:845	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	4	39	dep	factors	825:831	arg1	factors					825:831	modulated platelet-derived growth factors	791:831	modulated platelet-derived growth factors sequestration and release	791:857	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	4	39	dep	factors	825:831	arg1	release					851:857	release	851:857	release	851:857	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	5	40	theme	osteoinductive	1062:1075	arg1	properties					1077:1086	their osteoinductive properties	1056:1086	their osteoinductive properties	1056:1086	Furthermore, they increased alkaline phosphatase activity (up to 10-fold) and up-regulated the expression of bone-related markers (up to 2-fold), without osteogenic supplementation, demonstrating their osteoinductive properties.					
35725198	4	41	theme	75	752:753	arg1	%					754:754	%	754:754	%	754:754	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	3	42	theme	bone	533:536	arg1	nature					568:573	bone ECM protein-mineral composite nature	533:573	bone ECM protein-mineral composite nature	533:573	These mineralized-CNCs were combined with platelet lysate to produce nanocomposite scaffolds through cryogelation to mimic bone ECM protein-mineral composite nature and take advantage of the bioactivity steaming from platelet-derived biomolecules.					
35725198	4	43	theme	growth	818:823	arg1	sequestration					833:845	sequestration	833:845	sequestration	833:845	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	4	43	theme	growth	818:823	arg1	factors					825:831	modulated platelet-derived growth factors	791:831	modulated platelet-derived growth factors sequestration and release	791:857	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	4	43	theme	growth	818:823	arg1	release					851:857	release	851:857	release	851:857	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	5	44	dep	10-fold	925:931	arg1	to					922:923	to	922:923	to	922:923	Furthermore, they increased alkaline phosphatase activity (up to 10-fold) and up-regulated the expression of bone-related markers (up to 2-fold), without osteogenic supplementation, demonstrating their osteoinductive properties.					
35725198	4	45	theme	platelet-derived	801:816	arg1	sequestration					833:845	sequestration	833:845	sequestration	833:845	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	4	45	theme	platelet-derived	801:816	arg1	factors					825:831	modulated platelet-derived growth factors	791:831	modulated platelet-derived growth factors sequestration and release	791:857	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	4	45	theme	platelet-derived	801:816	arg1	release					851:857	release	851:857	release	851:857	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	1	46	theme	vascularized	133:144	arg1	Bone					123:126	Bone	123:126	Bone	123:126	Bone is a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale.					
35725198	1	46	theme	vascularized	133:144	arg1	tissue					174:179	a vascularized organic-inorganic composite tissue	131:179	a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale	131:256	Bone is a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale.					
35725198	4	47	theme	high	693:696	arg1	microporosity					698:710	high microporosity	693:710	high microporosity (94-95%)	693:719	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	4	47	theme	high	693:696	arg1	%					718:718	94-95%	713:718	94-95%	713:718	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	0	48	theme	platelet	64:71	arg1	lysate					73:78	platelet lysate	64:78	platelet lysate	64:78	Highly elastic and bioactive bone biomimetic scaffolds based on platelet lysate and biomineralized cellulose nanocrystals.					
35725198	6	49	theme	endothelial	1185:1195	arg1	markers					1197:1203	endothelial markers	1185:1203	endothelial markers	1185:1203	Also, the scaffolds promoted the chemotaxis of endothelial cells and enhanced the expression of endothelial markers, showing proangiogenic potential.					
35725198	1	50	theme	organic-inorganic	146:162	arg1	Bone					123:126	Bone	123:126	Bone	123:126	Bone is a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale.					
35725198	1	50	theme	organic-inorganic	146:162	arg1	tissue					174:179	a vascularized organic-inorganic composite tissue	131:179	a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale	131:256	Bone is a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale.					
35725198	3	51	link	platelet-derived	627:642	arg1	biomolecules					644:655	platelet-derived biomolecules	627:655	platelet-derived biomolecules	627:655	These mineralized-CNCs were combined with platelet lysate to produce nanocomposite scaffolds through cryogelation to mimic bone ECM protein-mineral composite nature and take advantage of the bioactivity steaming from platelet-derived biomolecules.					
35725198	4	52	dep	elasticity	727:736	arg1	recover					739:745	recover	739:745	recover from 75% strain cycles	739:768	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	1	53	theme	composite	164:172	arg1	Bone					123:126	Bone	123:126	Bone	123:126	Bone is a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale.					
35725198	1	53	theme	composite	164:172	arg1	tissue					174:179	a vascularized organic-inorganic composite tissue	131:179	a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale	131:256	Bone is a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale.					
35725198	4	54	from	cycles	763:768	arg1	recover					739:745	recover	739:745	recover from 75% strain cycles	739:768	The nanocomposite scaffolds showed high microporosity (94-95%), high elasticity (recover from 75% strain cycles), injectability, and modulated platelet-derived growth factors sequestration and release.					
35725198	7	55	theme	bone	1318:1321	arg1	regeneration					1323:1334	bone regeneration	1318:1334	bone regeneration	1318:1334	These results suggest that the mineralized nanocomposite scaffolds can enhance bone regeneration by simultaneously promoting osteogenesis and angiogenesis.					
37341384	3	0	theme	saccharides	809:819	arg1	spectra					784:790	room-temperature infrared spectra	758:790	room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1)	758:903	Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1) provide well-resolved and highly diagnostic features.					
37341384	6	1	theme	body	1445:1448	arg1	samples					1456:1462	patient body fluid samples	1437:1462	patient body fluid samples	1437:1462	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	1	2	theme	major	182:186	arg1	challenge					188:196	a major challenge	180:196	a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC-MS)	180:280	Distinguishing isomeric saccharides poses a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC-MS).					
37341384	3	3	theme	well-resolved	913:925	arg1	features					949:956	well-resolved and highly diagnostic features	913:956	well-resolved and highly diagnostic features	913:956	Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1) provide well-resolved and highly diagnostic features.					
37341384	0	4	theme	Biomarkers	94:103	arg1	Identification					76:89	Identification	76:89	Identification of Biomarkers for Inborn Errors of Metabolism	76:135	Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism.					
37341384	2	5	theme	infrared	665:672	arg1	spectra					674:680	their room-temperature fingerprint infrared spectra	630:680	their room-temperature fingerprint infrared spectra	630:680	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	2	6	theme	diagnostic	721:730	arg1	value					732:736	diagnostic value	721:736	diagnostic value	721:736	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	2	7	theme	possible	358:365	arg1	solution					367:374	a possible solution	356:374	a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value	356:736	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	2	7	theme	possible	358:365	arg1	spectroscopy					340:351	infrared ion spectroscopy	327:351	infrared ion spectroscopy	327:351	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	4	8	theme	saccharides	1009:1019	arg1	distinction					985:995	distinction	985:995	distinction of isomeric saccharides that differ either by their composition of monosaccharide units and/or the orientation of their glycosidic linkages	985:1135	We show that this enables distinction of isomeric saccharides that differ either by their composition of monosaccharide units and/or the orientation of their glycosidic linkages.					
37341384	2	9	dep	orthogonal	383:392	arg1	spectroscopic					395:407	spectroscopic	395:407	spectroscopic	395:407	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	2	10	theme	fingerprint	653:663	arg1	spectra					674:680	their room-temperature fingerprint infrared spectra	630:680	their room-temperature fingerprint infrared spectra	630:680	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	2	11	theme	many	300:303	arg1	studies					305:311	many studies	300:311	many studies	300:311	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	0	12	theme	Inborn	109:114	arg1	Errors					116:121	Inborn Errors	109:121	Inborn Errors of Metabolism	109:135	Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism.					
37341384	2	13	theme	room-temperature	636:651	arg1	spectra					674:680	their room-temperature fingerprint infrared spectra	630:680	their room-temperature fingerprint infrared spectra	630:680	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	6	14	with	hyphenation	1331:1341	arg1	chromatography					1379:1392	hydrophilic interaction liquid chromatography	1348:1392	hydrophilic interaction liquid chromatography	1348:1392	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	0	15	theme	Metabolism	126:135	arg1	Errors					116:121	Inborn Errors	109:121	Inborn Errors of Metabolism	109:135	Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism.					
37341384	3	16	theme	ion-complexed	795:807	arg1	saccharides					809:819	ion-complexed saccharides	795:819	ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1)	795:903	Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1) provide well-resolved and highly diagnostic features.					
37341384	6	17	theme	oligosaccharide	1407:1421	arg1	biomarkers					1423:1432	oligosaccharide biomarkers	1407:1432	oligosaccharide biomarkers	1407:1432	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	6	18	theme	sensitive	1504:1512	arg1	method					1523:1528	a generalized and highly sensitive MS-based method	1479:1528	a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices	1479:1599	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	3	19	theme	infrared	775:782	arg1	spectra					784:790	room-temperature infrared spectra	758:790	room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1)	758:903	Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1) provide well-resolved and highly diagnostic features.					
37341384	2	20	theme	broad	690:694	arg1	features					696:703	broad features	690:703	broad features that often lack diagnostic value	690:736	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	2	21	theme	recent	286:291	arg1	years					293:297	recent years	286:297	recent years	286:297	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	6	22	theme	sample	1585:1590	arg1	matrices					1592:1599	complex sample matrices	1577:1599	complex sample matrices	1577:1599	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	1	23	theme	analytical	202:211	arg1	workflows					213:221	analytical workflows	202:221	analytical workflows based on (liquid chromatography) mass spectrometry (LC-MS)	202:280	Distinguishing isomeric saccharides poses a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC-MS).					
37341384	0	24	theme	Oligosaccharide	15:29	arg1	Isomers					31:37	Oligosaccharide Isomers	15:37	Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy	15:73	Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism.					
37341384	4	25	theme	units	1079:1083	arg1	composition					1049:1059	their composition	1043:1059	their composition of monosaccharide units	1043:1083	We show that this enables distinction of isomeric saccharides that differ either by their composition of monosaccharide units and/or the orientation of their glycosidic linkages.					
37341384	2	26	theme	infrared	327:334	arg1	solution					367:374	a possible solution	356:374	a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value	356:736	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	2	26	theme	infrared	327:334	arg1	spectroscopy					340:351	infrared ion spectroscopy	327:351	infrared ion spectroscopy	327:351	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	5	27	theme	single	1283:1288	arg1	linkage					1301:1307	a single glycosidic linkage	1281:1307	a single glycosidic linkage	1281:1307	We demonstrate the utility of this approach from single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage.					
37341384	2	28	theme	isomeric	470:477	arg1	species					479:485	isomeric species	470:485	isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value	470:736	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	5	29	theme	glycosidic	1290:1299	arg1	linkage					1301:1307	a single glycosidic linkage	1281:1307	a single glycosidic linkage	1281:1307	We demonstrate the utility of this approach from single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage.					
37341384	4	30	theme	linkages	1128:1135	arg1	orientation					1096:1106	the orientation	1092:1106	the orientation of their glycosidic linkages	1092:1135	We show that this enables distinction of isomeric saccharides that differ either by their composition of monosaccharide units and/or the orientation of their glycosidic linkages.					
37341384	2	31	theme	orthogonal	383:392	arg1	characterization					409:424	the orthogonal, spectroscopic characterization	379:424	the orthogonal, spectroscopic characterization of mass-selected ions	379:446	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	5	32	theme	linkage	1301:1307	arg1	configuration					1264:1276	the configuration	1260:1276	the configuration of a single glycosidic linkage	1260:1307	We demonstrate the utility of this approach from single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage.					
37341384	6	33	theme	patient	1437:1443	arg1	samples					1456:1462	patient body fluid samples	1437:1462	patient body fluid samples	1437:1462	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	6	34	theme	saccharides	1556:1566	arg1	identification					1538:1551	the identification	1534:1551	the identification of saccharides found in complex sample matrices	1534:1599	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	2	35	dep	unresolved	499:508	arg1	using					510:514	using	510:514	using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value	510:736	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	2	36	theme	hydrogen	592:599	arg1	bonding					601:607	extensive hydrogen bonding	582:607	extensive hydrogen bonding in saccharides	582:622	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	5	37	theme	approach	1173:1180	arg1	utility					1157:1163	the utility	1153:1163	the utility of this approach from single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage	1153:1307	We demonstrate the utility of this approach from single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage.					
37341384	2	38	theme	ions	443:446	arg1	characterization					409:424	the orthogonal, spectroscopic characterization	379:424	the orthogonal, spectroscopic characterization of mass-selected ions	379:446	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	0	39	theme	Ion	58:60	arg1	Spectroscopy					62:73	Far-Infrared Ion Spectroscopy	45:73	Far-Infrared Ion Spectroscopy	45:73	Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism.					
37341384	2	40	theme	extensive	582:590	arg1	bonding					601:607	extensive hydrogen bonding	582:607	extensive hydrogen bonding in saccharides	582:622	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	2	41	theme	mass-selected	429:441	arg1	ions					443:446	mass-selected ions	429:446	mass-selected ions	429:446	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	1	42	theme	liquid	233:238	arg1	spectrometry					261:272	(liquid chromatography) mass spectrometry	232:272	(liquid chromatography) mass spectrometry (LC-MS)	232:280	Distinguishing isomeric saccharides poses a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC-MS).					
37341384	1	42	theme	liquid	233:238	arg1	LC-MS					275:279	LC-MS	275:279	LC-MS	275:279	Distinguishing isomeric saccharides poses a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC-MS).					
37341384	0	43	theme	Far-Infrared	45:56	arg1	Spectroscopy					62:73	Far-Infrared Ion Spectroscopy	45:73	Far-Infrared Ion Spectroscopy	45:73	Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism.					
37341384	3	44	theme	far-infrared	859:870	arg1	range					883:887	the previously unexplored far-infrared wavelength range	833:887	the previously unexplored far-infrared wavelength range (300-1000 cm-1)	833:903	Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1) provide well-resolved and highly diagnostic features.					
37341384	3	44	theme	far-infrared	859:870	arg1	cm-1					899:902	300-1000 cm-1	890:902	300-1000 cm-1	890:902	Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1) provide well-resolved and highly diagnostic features.					
37341384	6	45	theme	complex	1577:1583	arg1	matrices					1592:1599	complex sample matrices	1577:1599	complex sample matrices	1577:1599	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	5	46	theme	single	1187:1192	arg1	monosaccharides					1194:1208	single monosaccharides	1187:1208	single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage	1187:1307	We demonstrate the utility of this approach from single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage.					
37341384	6	47	theme	liquid	1372:1377	arg1	chromatography					1379:1392	hydrophilic interaction liquid chromatography	1348:1392	hydrophilic interaction liquid chromatography	1348:1392	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	1	48	theme	chromatography	240:253	arg1	spectrometry					261:272	(liquid chromatography) mass spectrometry	232:272	(liquid chromatography) mass spectrometry (LC-MS)	232:280	Distinguishing isomeric saccharides poses a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC-MS).					
37341384	1	48	theme	chromatography	240:253	arg1	LC-MS					275:279	LC-MS	275:279	LC-MS	275:279	Distinguishing isomeric saccharides poses a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC-MS).					
37341384	4	49	theme	glycosidic	1117:1126	arg1	linkages					1128:1135	their glycosidic linkages	1111:1135	their glycosidic linkages	1111:1135	We show that this enables distinction of isomeric saccharides that differ either by their composition of monosaccharide units and/or the orientation of their glycosidic linkages.					
37341384	3	50	theme	wavelength	872:881	arg1	range					883:887	the previously unexplored far-infrared wavelength range	833:887	the previously unexplored far-infrared wavelength range (300-1000 cm-1)	833:903	Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1) provide well-resolved and highly diagnostic features.					
37341384	3	50	theme	wavelength	872:881	arg1	cm-1					899:902	300-1000 cm-1	890:902	300-1000 cm-1	890:902	Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1) provide well-resolved and highly diagnostic features.					
37341384	6	51	theme	generalized	1481:1491	arg1	method					1523:1528	a generalized and highly sensitive MS-based method	1479:1528	a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices	1479:1599	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	6	52	theme	interaction	1360:1370	arg1	chromatography					1379:1392	hydrophilic interaction liquid chromatography	1348:1392	hydrophilic interaction liquid chromatography	1348:1392	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	5	53	theme	isomeric	1216:1223	arg1	tetrasaccharides					1225:1240	isomeric tetrasaccharides	1216:1240	isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage	1216:1307	We demonstrate the utility of this approach from single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage.					
37341384	2	54	dep	conventional	516:527	arg1	cause					624:628	cause	624:628	cause their room-temperature fingerprint infrared spectra	624:680	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	6	55	theme	hydrophilic	1348:1358	arg1	chromatography					1379:1392	hydrophilic interaction liquid chromatography	1348:1392	hydrophilic interaction liquid chromatography	1348:1392	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	6	56	located	found	1568:1572	arg2	saccharides					1556:1566	saccharides	1556:1566	saccharides found in complex sample matrices	1556:1599	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	6	56	located	found	1568:1572	arg1	matrices					1592:1599	complex sample matrices	1577:1599	complex sample matrices	1577:1599	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	3	57	theme	unexplored	848:857	arg1	range					883:887	the previously unexplored far-infrared wavelength range	833:887	the previously unexplored far-infrared wavelength range (300-1000 cm-1)	833:903	Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1) provide well-resolved and highly diagnostic features.					
37341384	3	57	theme	unexplored	848:857	arg1	cm-1					899:902	300-1000 cm-1	890:902	300-1000 cm-1	890:902	Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1) provide well-resolved and highly diagnostic features.					
37341384	4	58	theme	isomeric	1000:1007	arg1	saccharides					1009:1019	isomeric saccharides	1000:1019	isomeric saccharides that differ either by their composition of monosaccharide units and/or the orientation of their glycosidic linkages	1000:1135	We show that this enables distinction of isomeric saccharides that differ either by their composition of monosaccharide units and/or the orientation of their glycosidic linkages.					
37341384	1	59	theme	isomeric	153:160	arg1	saccharides					162:172	isomeric saccharides	153:172	isomeric saccharides	153:172	Distinguishing isomeric saccharides poses a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC-MS).					
37341384	6	60	theme	MS-based	1514:1521	arg1	method					1523:1528	a generalized and highly sensitive MS-based method	1479:1528	a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices	1479:1599	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	6	61	theme	fluid	1450:1454	arg1	samples					1456:1462	patient body fluid samples	1437:1462	patient body fluid samples	1437:1462	Furthermore, through hyphenation with hydrophilic interaction liquid chromatography, we identify oligosaccharide biomarkers in patient body fluid samples, demonstrating a generalized and highly sensitive MS-based method for the identification of saccharides found in complex sample matrices.					
37341384	2	62	from	flexibility	566:576	arg1	saccharides					612:622	saccharides	612:622	saccharides	612:622	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	2	63	dep	solution	367:374	arg1	distinguish					458:468	distinguish	458:468	can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value	448:736	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	2	64	theme	ion	336:338	arg1	solution					367:374	a possible solution	356:374	a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value	356:736	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	2	64	theme	ion	336:338	arg1	spectroscopy					340:351	infrared ion spectroscopy	327:351	infrared ion spectroscopy	327:351	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	5	65	from	monosaccharides	1194:1208	arg1	utility					1157:1163	the utility	1153:1163	the utility of this approach from single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage	1153:1307	We demonstrate the utility of this approach from single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage.					
37341384	1	66	theme	mass	256:259	arg1	spectrometry					261:272	(liquid chromatography) mass spectrometry	232:272	(liquid chromatography) mass spectrometry (LC-MS)	232:280	Distinguishing isomeric saccharides poses a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC-MS).					
37341384	1	66	theme	mass	256:259	arg1	LC-MS					275:279	LC-MS	275:279	LC-MS	275:279	Distinguishing isomeric saccharides poses a major challenge for analytical workflows based on (liquid chromatography) mass spectrometry (LC-MS).					
37341384	2	67	theme	high	546:549	arg1	flexibility					566:576	the high conformational flexibility	542:576	the high conformational flexibility	542:576	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	3	68	theme	diagnostic	938:947	arg1	features					949:956	well-resolved and highly diagnostic features	913:956	well-resolved and highly diagnostic features	913:956	Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1) provide well-resolved and highly diagnostic features.					
37341384	2	69	from	bonding	601:607	arg1	saccharides					612:622	saccharides	612:622	saccharides	612:622	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
37341384	4	70	theme	monosaccharide	1064:1077	arg1	units					1079:1083	monosaccharide units	1064:1083	monosaccharide units	1064:1083	We show that this enables distinction of isomeric saccharides that differ either by their composition of monosaccharide units and/or the orientation of their glycosidic linkages.					
37341384	5	71	dep	tetrasaccharides	1225:1240	arg1	up					1210:1211	up	1210:1211	up	1210:1211	We demonstrate the utility of this approach from single monosaccharides up to isomeric tetrasaccharides differing only by the configuration of a single glycosidic linkage.					
37341384	0	72	dep	Distinguishing	0:13	arg1	Identification					76:89	Identification	76:89	Identification of Biomarkers for Inborn Errors of Metabolism	76:135	Distinguishing Oligosaccharide Isomers Using Far-Infrared Ion Spectroscopy: Identification of Biomarkers for Inborn Errors of Metabolism.					
37341384	3	73	theme	room-temperature	758:773	arg1	spectra					784:790	room-temperature infrared spectra	758:790	room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1)	758:903	Here, we show that room-temperature infrared spectra of ion-complexed saccharides recorded in the previously unexplored far-infrared wavelength range (300-1000 cm-1) provide well-resolved and highly diagnostic features.					
37341384	2	74	theme	conformational	551:564	arg1	flexibility					566:576	the high conformational flexibility	542:576	the high conformational flexibility	542:576	In recent years, many studies have proposed infrared ion spectroscopy as a possible solution as the orthogonal, spectroscopic characterization of mass-selected ions can often distinguish isomeric species that remain unresolved using conventional MS. However, the high conformational flexibility and extensive hydrogen bonding in saccharides cause their room-temperature fingerprint infrared spectra to have broad features that often lack diagnostic value.					
35598976	0	0	theme	in	111:112	arg1	Evaluation					119:128	in Vitro and in Vivo Evaluation	98:128	in Vitro and in Vivo Evaluation	98:128	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.					
35598976	0	0	theme	in	111:112	arg1	Preparation					71:81	Preparation	71:81	Preparation	71:81	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.					
35598976	1	1	with	product	163:169	arg1	effects					200:206	various pharmacological effects	176:206	various pharmacological effects	176:206	Andrographolide (AG), a natural product with various pharmacological effects, exhibited low oral bioavailability owing to its poor solubility, stability, and low absorption.					
35598976	4	2	theme	combined	701:708	arg1	methodology					727:737	central composite design combined response surface methodology	676:737	central composite design combined response surface methodology	676:737	Therefore, AG/HPCD/PC complex (AHPC) was synthesized, and AHPC-loaded nanoemulsion (AHPC-NE) was optimized and prepared using central composite design combined response surface methodology.					
35598976	8	3	theme	relative	1182:1189	arg1	bioavailability					1191:1205	a relative bioavailability	1180:1205	a relative bioavailability of 550.71%	1180:1216	Further, AHPC-NE significantly enhanced the absorption of AG with a relative bioavailability of 550.71% compared to AG suspension.					
35598976	9	4	dep	sustained-release	1302:1318	arg1	enhancement					1345:1355	enhancement	1345:1355	enhancement	1345:1355	Such findings reveal AHPC-NE as a potential strategy for sustained-release and oral bioavailability enhancement.					
35598976	3	5	theme	appropriate	503:513	arg1	enhancer					515:522	an appropriate enhancer	500:522	an appropriate enhancer for oral bioavailability	500:547	Moreover, nanoemulsion (NE) has been confirmed as an appropriate enhancer for oral bioavailability.					
35598976	3	5	theme	appropriate	503:513	arg1	nanoemulsion					460:471	nanoemulsion	460:471	nanoemulsion (NE)	460:476	Moreover, nanoemulsion (NE) has been confirmed as an appropriate enhancer for oral bioavailability.					
35598976	8	6	with	absorption	1158:1167	arg1	bioavailability					1191:1205	a relative bioavailability	1180:1205	a relative bioavailability of 550.71%	1180:1216	Further, AHPC-NE significantly enhanced the absorption of AG with a relative bioavailability of 550.71% compared to AG suspension.					
35598976	7	7	theme	good	1082:1085	arg1	effects					1105:1111	good sustained-release effects	1082:1111	good sustained-release effects	1082:1111	In vivo release studies demonstrated that AHPC-NE had good sustained-release effects.					
35598976	2	8	theme	Previous	305:312	arg1	studies					314:320	Previous studies	305:320	Previous studies	305:320	Previous studies have suggested that phospholipid (PC) and hydroxypropyl-β-cyclodextrin (HPCD) could improve the drug solubility and absorption.					
35598976	5	9	theme	average	744:750	arg1	PDI					791:793	PDI	791:793	PDI	791:793	The average droplet size and polydispersity index (PDI) were 116.50 ± 5.99 and 0.29 ± 0.03 nm, respectively.					
35598976	5	9	theme	average	744:750	arg1	size					760:763	average droplet size	744:763	average droplet size	744:763	The average droplet size and polydispersity index (PDI) were 116.50 ± 5.99 and 0.29 ± 0.03 nm, respectively.					
35598976	6	10	theme	encapsulation	904:916	arg1	efficiency					918:927	an encapsulation efficiency	901:927	an encapsulation efficiency of 96.43 ± 2.27%	901:944	AHPC-NE with a loading capacity of 0.32 ± 0.01% and an encapsulation efficiency of 96.43 ± 2.27% appeared round and uniformly dispersed based on transmission electron microscopy.					
35598976	6	11	theme	electron	1007:1014	arg1	microscopy					1016:1025	transmission electron microscopy	994:1025	transmission electron microscopy	994:1025	AHPC-NE with a loading capacity of 0.32 ± 0.01% and an encapsulation efficiency of 96.43 ± 2.27% appeared round and uniformly dispersed based on transmission electron microscopy.					
35598976	4	12	theme	design	694:699	arg1	methodology					727:737	central composite design combined response surface methodology	676:737	central composite design combined response surface methodology	676:737	Therefore, AG/HPCD/PC complex (AHPC) was synthesized, and AHPC-loaded nanoemulsion (AHPC-NE) was optimized and prepared using central composite design combined response surface methodology.					
35598976	3	13	theme	oral	528:531	arg1	bioavailability					533:547	oral bioavailability	528:547	oral bioavailability	528:547	Moreover, nanoemulsion (NE) has been confirmed as an appropriate enhancer for oral bioavailability.					
35598976	1	14	theme	various	176:182	arg1	effects					200:206	various pharmacological effects	176:206	various pharmacological effects	176:206	Andrographolide (AG), a natural product with various pharmacological effects, exhibited low oral bioavailability owing to its poor solubility, stability, and low absorption.					
35598976	8	15	theme	%	1216:1216	arg1	bioavailability					1191:1205	a relative bioavailability	1180:1205	a relative bioavailability of 550.71%	1180:1216	Further, AHPC-NE significantly enhanced the absorption of AG with a relative bioavailability of 550.71% compared to AG suspension.					
35598976	9	16	theme	Such	1245:1248	arg1	findings					1250:1257	Such findings	1245:1257	Such findings	1245:1257	Such findings reveal AHPC-NE as a potential strategy for sustained-release and oral bioavailability enhancement.					
35598976	1	17	theme	pharmacological	184:198	arg1	effects					200:206	various pharmacological effects	176:206	various pharmacological effects	176:206	Andrographolide (AG), a natural product with various pharmacological effects, exhibited low oral bioavailability owing to its poor solubility, stability, and low absorption.					
35598976	0	18	theme	Complex-Loaded	42:55	arg1	Nanoemulsion					57:68	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion	0:68	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.	0:129	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.					
35598976	6	19	theme	%	895:895	arg1	capacity					872:879	a loading capacity	862:879	a loading capacity of 0.32 ± 0.01%	862:895	AHPC-NE with a loading capacity of 0.32 ± 0.01% and an encapsulation efficiency of 96.43 ± 2.27% appeared round and uniformly dispersed based on transmission electron microscopy.					
35598976	6	20	theme	transmission	994:1005	arg1	microscopy					1016:1025	transmission electron microscopy	994:1025	transmission electron microscopy	994:1025	AHPC-NE with a loading capacity of 0.32 ± 0.01% and an encapsulation efficiency of 96.43 ± 2.27% appeared round and uniformly dispersed based on transmission electron microscopy.					
35598976	0	21	dep	in	111:112	arg1	Vivo					114:117	Vivo	114:117	Vivo	114:117	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.					
35598976	7	22	contain	had	1078:1080	arg2	effects					1105:1111	good sustained-release effects	1082:1111	good sustained-release effects	1082:1111	In vivo release studies demonstrated that AHPC-NE had good sustained-release effects.					
35598976	7	22	contain	had	1078:1080	arg1	AHPC-NE					1070:1076	AHPC-NE	1070:1076	AHPC-NE	1070:1076	In vivo release studies demonstrated that AHPC-NE had good sustained-release effects.					
35598976	0	23	theme	Andrographolide/Phospholipid/Cyclodextrin	0:40	arg1	Nanoemulsion					57:68	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion	0:68	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.	0:129	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.					
35598976	4	24	theme	AG/HPCD/PC	561:570	arg1	AHPC					581:584	AHPC	581:584	AHPC	581:584	Therefore, AG/HPCD/PC complex (AHPC) was synthesized, and AHPC-loaded nanoemulsion (AHPC-NE) was optimized and prepared using central composite design combined response surface methodology.					
35598976	4	24	theme	AG/HPCD/PC	561:570	arg1	complex					572:578	AG/HPCD/PC complex	561:578	AG/HPCD/PC complex (AHPC)	561:585	Therefore, AG/HPCD/PC complex (AHPC) was synthesized, and AHPC-loaded nanoemulsion (AHPC-NE) was optimized and prepared using central composite design combined response surface methodology.					
35598976	1	25	theme	low	289:291	arg1	absorption					293:302	low absorption	289:302	low absorption	289:302	Andrographolide (AG), a natural product with various pharmacological effects, exhibited low oral bioavailability owing to its poor solubility, stability, and low absorption.					
35598976	6	26	with	AHPC-NE	849:855	arg1	capacity					872:879	a loading capacity	862:879	a loading capacity of 0.32 ± 0.01%	862:895	AHPC-NE with a loading capacity of 0.32 ± 0.01% and an encapsulation efficiency of 96.43 ± 2.27% appeared round and uniformly dispersed based on transmission electron microscopy.					
35598976	0	27	dep	in	98:99	arg1	Vitro					101:105	Vitro	101:105	Vitro	101:105	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.					
35598976	1	28	theme	low	219:221	arg1	bioavailability					228:242	low oral bioavailability	219:242	low oral bioavailability owing to its poor solubility, stability, and low absorption	219:302	Andrographolide (AG), a natural product with various pharmacological effects, exhibited low oral bioavailability owing to its poor solubility, stability, and low absorption.					
35598976	6	29	with	efficiency	918:927	arg1	capacity					872:879	a loading capacity	862:879	a loading capacity of 0.32 ± 0.01%	862:895	AHPC-NE with a loading capacity of 0.32 ± 0.01% and an encapsulation efficiency of 96.43 ± 2.27% appeared round and uniformly dispersed based on transmission electron microscopy.					
35598976	7	30	theme	sustained-release	1087:1103	arg1	effects					1105:1111	good sustained-release effects	1082:1111	good sustained-release effects	1082:1111	In vivo release studies demonstrated that AHPC-NE had good sustained-release effects.					
35598976	1	31	theme	oral	223:226	arg1	bioavailability					228:242	low oral bioavailability	219:242	low oral bioavailability owing to its poor solubility, stability, and low absorption	219:302	Andrographolide (AG), a natural product with various pharmacological effects, exhibited low oral bioavailability owing to its poor solubility, stability, and low absorption.					
35598976	7	32	dep	In	1028:1029	arg1	vivo					1031:1034	vivo	1031:1034	vivo	1031:1034	In vivo release studies demonstrated that AHPC-NE had good sustained-release effects.					
35598976	2	33	theme	drug	418:421	arg1	solubility					423:432	the drug solubility	414:432	the drug solubility	414:432	Previous studies have suggested that phospholipid (PC) and hydroxypropyl-β-cyclodextrin (HPCD) could improve the drug solubility and absorption.					
35598976	9	34	theme	potential	1279:1287	arg1	strategy					1289:1296	a potential strategy	1277:1296	a potential strategy for sustained-release and oral bioavailability enhancement	1277:1355	Such findings reveal AHPC-NE as a potential strategy for sustained-release and oral bioavailability enhancement.					
35598976	9	34	theme	potential	1279:1287	arg1	AHPC-NE					1266:1272	AHPC-NE	1266:1272	AHPC-NE	1266:1272	Such findings reveal AHPC-NE as a potential strategy for sustained-release and oral bioavailability enhancement.					
35598976	8	35	theme	AG	1230:1231	arg1	suspension					1233:1242	AG suspension	1230:1242	AG suspension	1230:1242	Further, AHPC-NE significantly enhanced the absorption of AG with a relative bioavailability of 550.71% compared to AG suspension.					
35598976	6	36	theme	loading	864:870	arg1	capacity					872:879	a loading capacity	862:879	a loading capacity of 0.32 ± 0.01%	862:895	AHPC-NE with a loading capacity of 0.32 ± 0.01% and an encapsulation efficiency of 96.43 ± 2.27% appeared round and uniformly dispersed based on transmission electron microscopy.					
35598976	4	37	theme	surface	719:725	arg1	methodology					727:737	central composite design combined response surface methodology	676:737	central composite design combined response surface methodology	676:737	Therefore, AG/HPCD/PC complex (AHPC) was synthesized, and AHPC-loaded nanoemulsion (AHPC-NE) was optimized and prepared using central composite design combined response surface methodology.					
35598976	5	38	dep	size	760:763	arg1	The					740:742	The	740:742	The	740:742	The average droplet size and polydispersity index (PDI) were 116.50 ± 5.99 and 0.29 ± 0.03 nm, respectively.					
35598976	5	38	dep	size	760:763	arg1	index					784:788	index	784:788	index	784:788	The average droplet size and polydispersity index (PDI) were 116.50 ± 5.99 and 0.29 ± 0.03 nm, respectively.					
35598976	4	39	theme	response	710:717	arg1	methodology					727:737	central composite design combined response surface methodology	676:737	central composite design combined response surface methodology	676:737	Therefore, AG/HPCD/PC complex (AHPC) was synthesized, and AHPC-loaded nanoemulsion (AHPC-NE) was optimized and prepared using central composite design combined response surface methodology.					
35598976	8	40	theme	AG	1172:1173	arg1	absorption					1158:1167	the absorption	1154:1167	the absorption of AG with a relative bioavailability of 550.71%	1154:1216	Further, AHPC-NE significantly enhanced the absorption of AG with a relative bioavailability of 550.71% compared to AG suspension.					
35598976	7	41	theme	release	1036:1042	arg1	studies					1044:1050	In vivo release studies	1028:1050	In vivo release studies	1028:1050	In vivo release studies demonstrated that AHPC-NE had good sustained-release effects.					
35598976	5	42	theme	droplet	752:758	arg1	PDI					791:793	PDI	791:793	PDI	791:793	The average droplet size and polydispersity index (PDI) were 116.50 ± 5.99 and 0.29 ± 0.03 nm, respectively.					
35598976	5	42	theme	droplet	752:758	arg1	size					760:763	average droplet size	744:763	average droplet size	744:763	The average droplet size and polydispersity index (PDI) were 116.50 ± 5.99 and 0.29 ± 0.03 nm, respectively.					
35598976	0	43	dep	Nanoemulsion	57:68	arg1	Evaluation					119:128	in Vitro and in Vivo Evaluation	98:128	in Vitro and in Vivo Evaluation	98:128	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.					
35598976	0	43	dep	Nanoemulsion	57:68	arg1	Optimization					84:95	Optimization	84:95	Optimization	84:95	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.					
35598976	0	43	dep	Nanoemulsion	57:68	arg1	Preparation					71:81	Preparation	71:81	Preparation	71:81	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.					
35598976	4	44	theme	central	676:682	arg1	methodology					727:737	central composite design combined response surface methodology	676:737	central composite design combined response surface methodology	676:737	Therefore, AG/HPCD/PC complex (AHPC) was synthesized, and AHPC-loaded nanoemulsion (AHPC-NE) was optimized and prepared using central composite design combined response surface methodology.					
35598976	9	45	theme	oral	1324:1327	arg1	bioavailability					1329:1343	oral bioavailability	1324:1343	oral bioavailability	1324:1343	Such findings reveal AHPC-NE as a potential strategy for sustained-release and oral bioavailability enhancement.					
35598976	7	46	theme	In	1028:1029	arg1	studies					1044:1050	In vivo release studies	1028:1050	In vivo release studies	1028:1050	In vivo release studies demonstrated that AHPC-NE had good sustained-release effects.					
35598976	4	47	theme	composite	684:692	arg1	methodology					727:737	central composite design combined response surface methodology	676:737	central composite design combined response surface methodology	676:737	Therefore, AG/HPCD/PC complex (AHPC) was synthesized, and AHPC-loaded nanoemulsion (AHPC-NE) was optimized and prepared using central composite design combined response surface methodology.					
35598976	0	48	theme	in	98:99	arg1	Evaluation					119:128	in Vitro and in Vivo Evaluation	98:128	in Vitro and in Vivo Evaluation	98:128	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.					
35598976	0	48	theme	in	98:99	arg1	Preparation					71:81	Preparation	71:81	Preparation	71:81	Andrographolide/Phospholipid/Cyclodextrin Complex-Loaded Nanoemulsion: Preparation, Optimization, in Vitro and in Vivo Evaluation.					
35598976	6	49	theme	%	944:944	arg1	AHPC-NE					849:855	AHPC-NE	849:855	AHPC-NE with a loading capacity of 0.32 ± 0.01%	849:895	AHPC-NE with a loading capacity of 0.32 ± 0.01% and an encapsulation efficiency of 96.43 ± 2.27% appeared round and uniformly dispersed based on transmission electron microscopy.					
35598976	6	49	theme	%	944:944	arg1	efficiency					918:927	an encapsulation efficiency	901:927	an encapsulation efficiency of 96.43 ± 2.27%	901:944	AHPC-NE with a loading capacity of 0.32 ± 0.01% and an encapsulation efficiency of 96.43 ± 2.27% appeared round and uniformly dispersed based on transmission electron microscopy.					
35598976	4	50	theme	AHPC-loaded	608:618	arg1	AHPC-NE					634:640	AHPC-NE	634:640	AHPC-NE	634:640	Therefore, AG/HPCD/PC complex (AHPC) was synthesized, and AHPC-loaded nanoemulsion (AHPC-NE) was optimized and prepared using central composite design combined response surface methodology.					
35598976	4	50	theme	AHPC-loaded	608:618	arg1	nanoemulsion					620:631	AHPC-loaded nanoemulsion	608:631	AHPC-loaded nanoemulsion (AHPC-NE)	608:641	Therefore, AG/HPCD/PC complex (AHPC) was synthesized, and AHPC-loaded nanoemulsion (AHPC-NE) was optimized and prepared using central composite design combined response surface methodology.					
35598976	1	51	theme	natural	155:161	arg1	product					163:169	a natural product	153:169	a natural product with various pharmacological effects	153:206	Andrographolide (AG), a natural product with various pharmacological effects, exhibited low oral bioavailability owing to its poor solubility, stability, and low absorption.					
35598976	1	51	theme	natural	155:161	arg1	Andrographolide					131:145	Andrographolide	131:145	Andrographolide (AG)	131:150	Andrographolide (AG), a natural product with various pharmacological effects, exhibited low oral bioavailability owing to its poor solubility, stability, and low absorption.					
35598976	1	52	theme	poor	257:260	arg1	solubility					262:271	its poor solubility	253:271	its poor solubility	253:271	Andrographolide (AG), a natural product with various pharmacological effects, exhibited low oral bioavailability owing to its poor solubility, stability, and low absorption.					
36577138	0	0	theme	RBCs	94:97	arg1	Cryopreservation					68:83	Cryopreservation	68:83	Cryopreservation of Human RBCs	68:97	Integration of Trehalose Lipids with Dissociative Trehalose Enables Cryopreservation of Human RBCs.					
36577138	4	1	from	comparison	660:669	arg1	able					741:744	able	741:744	able	741:744	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	4	2	theme	cell	757:760	arg1	membranes					762:770	cell membranes	757:770	cell membranes	757:770	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	0	3	theme	Human	88:92	arg1	RBCs					94:97	Human RBCs	88:97	Human RBCs	88:97	Integration of Trehalose Lipids with Dissociative Trehalose Enables Cryopreservation of Human RBCs.					
36577138	5	4	theme	cell	943:946	arg1	morphology					948:957	cell morphology	943:957	cell morphology	943:957	Moreover, there were slight changes in cell morphology and cell functions.					
36577138	3	5	theme	synthesized	543:553	arg1	lipids					565:570	the synthesized trehalose lipids	539:570	the synthesized trehalose lipids	539:570	Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization.					
36577138	1	6	theme	freezing	256:263	arg1	temperatures					265:276	freezing temperatures	256:276	freezing temperatures	256:276	Cryopreservation of red blood cells (RBCs) is imperative for transfusion therapy, while cryoprotectants are essential to protect RBCs from cryoinjury under freezing temperatures.					
36577138	7	7	theme	RBCs	1291:1294	arg1	types					1306:1310	other types	1300:1310	other types of cells	1300:1319	The aforementioned study is likely to provide an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells.					
36577138	7	7	theme	RBCs	1291:1294	arg1	cryopreservation					1265:1280	glycerol-free cryopreservation	1251:1280	glycerol-free cryopreservation of human RBCs	1251:1294	The aforementioned study is likely to provide an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells.					
36577138	7	8	theme	aforementioned	1186:1199	arg1	study					1201:1205	The aforementioned study	1182:1205	The aforementioned study	1182:1205	The aforementioned study is likely to provide an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells.					
36577138	7	8	theme	aforementioned	1186:1199	arg1	likely					1210:1215	likely	1210:1215	likely	1210:1215	The aforementioned study is likely to provide an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells.					
36577138	4	9	with	comparison	660:669	arg1	trehalose					699:707	trehalose	699:707	trehalose only	699:712	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	4	9	with	comparison	660:669	arg1	monolaurate					684:694	sucrose monolaurate	676:694	sucrose monolaurate	676:694	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	7	10	theme	other	1300:1304	arg1	types					1306:1310	other types	1300:1310	other types of cells	1300:1319	The aforementioned study is likely to provide an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells.					
36577138	4	11	theme	96.9	805:808	arg1	±					810:810	±	810:810	±	810:810	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	6	12	theme	osmotic	1044:1050	arg1	fragility					1052:1060	osmotic fragility	1044:1060	osmotic fragility	1044:1060	It was further confirmed by isothermal titration calorimetry and osmotic fragility tests that the moderate membrane-binding activity of trehalose lipids exerted cell stabilization for high cryosurvival.					
36577138	5	13	from	changes	932:938	arg1	morphology					948:957	cell morphology	943:957	cell morphology	943:957	Moreover, there were slight changes in cell morphology and cell functions.					
36577138	5	13	from	changes	932:938	arg1	functions					968:976	cell functions	963:976	cell functions	963:976	Moreover, there were slight changes in cell morphology and cell functions.					
36577138	4	14	theme	2.0	812:814	arg1	±					810:810	±	810:810	±	810:810	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	4	15	with	incubation	836:845	arg1	trehalose					893:901	0.8 M trehalose	887:901	0.8 M trehalose	887:901	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	6	16	theme	trehalose	1115:1123	arg1	lipids					1125:1130	trehalose lipids	1115:1130	trehalose lipids	1115:1130	It was further confirmed by isothermal titration calorimetry and osmotic fragility tests that the moderate membrane-binding activity of trehalose lipids exerted cell stabilization for high cryosurvival.					
36577138	6	17	theme	cell	1140:1143	arg1	stabilization					1145:1157	cell stabilization	1140:1157	cell stabilization	1140:1157	It was further confirmed by isothermal titration calorimetry and osmotic fragility tests that the moderate membrane-binding activity of trehalose lipids exerted cell stabilization for high cryosurvival.					
36577138	1	18	theme	transfusion	161:171	arg1	therapy					173:179	transfusion therapy	161:179	transfusion therapy	161:179	Cryopreservation of red blood cells (RBCs) is imperative for transfusion therapy, while cryoprotectants are essential to protect RBCs from cryoinjury under freezing temperatures.					
36577138	7	19	theme	human	1285:1289	arg1	RBCs					1291:1294	human RBCs	1285:1294	human RBCs	1285:1294	The aforementioned study is likely to provide an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells.					
36577138	4	20	theme	M	891:891	arg1	trehalose					893:901	0.8 M trehalose	887:901	0.8 M trehalose	887:901	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	3	21	theme	dissociative	576:587	arg1	trehalose					589:597	dissociative trehalose	576:597	dissociative trehalose	576:597	Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization.					
36577138	6	22	theme	high	1163:1166	arg1	cryosurvival					1168:1179	high cryosurvival	1163:1179	high cryosurvival	1163:1179	It was further confirmed by isothermal titration calorimetry and osmotic fragility tests that the moderate membrane-binding activity of trehalose lipids exerted cell stabilization for high cryosurvival.					
36577138	7	23	theme	glycerol-free	1251:1263	arg1	cryopreservation					1265:1280	glycerol-free cryopreservation	1251:1280	glycerol-free cryopreservation of human RBCs	1251:1294	The aforementioned study is likely to provide an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells.					
36577138	4	24	dep	cryosurvival	817:828	arg1	%					815:815	%	815:815	%	815:815	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	3	25	theme	feasible	445:452	arg1	protocol					454:461	a feasible protocol	443:461	a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization	443:643	Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization.					
36577138	7	26	theme	alternative	1231:1241	arg1	way					1243:1245	an alternative way	1228:1245	an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells	1228:1319	The aforementioned study is likely to provide an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells.					
36577138	2	27	theme	biocompatible	314:326	arg1	Trehalose					279:287	Trehalose	279:287	Trehalose	279:287	Trehalose has been considered as a biocompatible cryoprotectant that naturally accumulates in organisms to tolerate anhydrobiosis and cryobiosis.					
36577138	2	27	theme	biocompatible	314:326	arg1	cryoprotectant					328:341	a biocompatible cryoprotectant	312:341	a biocompatible cryoprotectant that naturally accumulates in organisms to tolerate anhydrobiosis and cryobiosis	312:422	Trehalose has been considered as a biocompatible cryoprotectant that naturally accumulates in organisms to tolerate anhydrobiosis and cryobiosis.					
36577138	5	28	theme	slight	925:930	arg1	changes					932:938	slight changes	925:938	slight changes in cell morphology and cell functions	925:976	Moreover, there were slight changes in cell morphology and cell functions.					
36577138	0	29	theme	Lipids	25:30	arg1	Integration					0:10	Integration	0:10	Integration of Trehalose Lipids with Dissociative Trehalose	0:58	Integration of Trehalose Lipids with Dissociative Trehalose Enables Cryopreservation of Human RBCs.					
36577138	6	30	theme	titration	1018:1026	arg1	calorimetry					1028:1038	isothermal titration calorimetry	1007:1038	isothermal titration calorimetry	1007:1038	It was further confirmed by isothermal titration calorimetry and osmotic fragility tests that the moderate membrane-binding activity of trehalose lipids exerted cell stabilization for high cryosurvival.					
36577138	3	31	theme	ice	607:609	arg1	tuning					611:616	ice tuning	607:616	ice tuning	607:616	Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization.					
36577138	6	32	theme	lipids	1125:1130	arg1	activity					1103:1110	the moderate membrane-binding activity	1073:1110	the moderate membrane-binding activity of trehalose lipids	1073:1130	It was further confirmed by isothermal titration calorimetry and osmotic fragility tests that the moderate membrane-binding activity of trehalose lipids exerted cell stabilization for high cryosurvival.					
36577138	3	33	theme	membrane	622:629	arg1	stabilization					631:643	membrane stabilization	622:643	membrane stabilization	622:643	Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization.					
36577138	6	34	theme	isothermal	1007:1016	arg1	calorimetry					1028:1038	isothermal titration calorimetry	1007:1038	isothermal titration calorimetry	1007:1038	It was further confirmed by isothermal titration calorimetry and osmotic fragility tests that the moderate membrane-binding activity of trehalose lipids exerted cell stabilization for high cryosurvival.					
36577138	1	35	theme	red	120:122	arg1	RBCs					137:140	RBCs	137:140	RBCs	137:140	Cryopreservation of red blood cells (RBCs) is imperative for transfusion therapy, while cryoprotectants are essential to protect RBCs from cryoinjury under freezing temperatures.					
36577138	1	35	theme	red	120:122	arg1	cells					130:134	red blood cells	120:134	red blood cells (RBCs)	120:141	Cryopreservation of red blood cells (RBCs) is imperative for transfusion therapy, while cryoprotectants are essential to protect RBCs from cryoinjury under freezing temperatures.					
36577138	3	36	theme	human	510:514	arg1	RBCs					516:519	human RBCs	510:519	human RBCs	510:519	Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization.					
36577138	0	37	theme	Dissociative	37:48	arg1	Trehalose					50:58	Dissociative Trehalose	37:58	Dissociative Trehalose	37:58	Integration of Trehalose Lipids with Dissociative Trehalose Enables Cryopreservation of Human RBCs.					
36577138	5	38	theme	cell	963:966	arg1	functions					968:976	cell functions	963:976	cell functions	963:976	Moreover, there were slight changes in cell morphology and cell functions.					
36577138	4	39	theme	RBCs	877:880	arg1	cryopreservation					851:866	cryopreservation	851:866	cryopreservation	851:866	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	4	39	theme	RBCs	877:880	arg1	incubation					836:845	incubation	836:845	incubation	836:845	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	3	40	theme	glycerol-free	476:488	arg1	cryopreservation					490:505	glycerol-free cryopreservation	476:505	glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids	476:570	Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization.					
36577138	4	41	theme	trehalose	715:723	arg1	monolaurate					725:735	trehalose monolaurate	715:735	trehalose monolaurate	715:735	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	4	42	with	cryopreservation	851:866	arg1	trehalose					893:901	0.8 M trehalose	887:901	0.8 M trehalose	887:901	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	4	43	theme	human	871:875	arg1	RBCs					877:880	human RBCs	871:880	human RBCs	871:880	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	7	44	theme	cells	1315:1319	arg1	types					1306:1310	other types	1300:1310	other types of cells	1300:1319	The aforementioned study is likely to provide an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells.					
36577138	7	44	theme	cells	1315:1319	arg1	cryopreservation					1265:1280	glycerol-free cryopreservation	1251:1280	glycerol-free cryopreservation of human RBCs	1251:1294	The aforementioned study is likely to provide an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells.					
36577138	4	45	theme	0.8	887:889	arg1	M					891:891	M	891:891	M	891:891	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	1	46	theme	blood	124:128	arg1	RBCs					137:140	RBCs	137:140	RBCs	137:140	Cryopreservation of red blood cells (RBCs) is imperative for transfusion therapy, while cryoprotectants are essential to protect RBCs from cryoinjury under freezing temperatures.					
36577138	1	46	theme	blood	124:128	arg1	cells					130:134	red blood cells	120:134	red blood cells (RBCs)	120:141	Cryopreservation of red blood cells (RBCs) is imperative for transfusion therapy, while cryoprotectants are essential to protect RBCs from cryoinjury under freezing temperatures.					
36577138	1	47	theme	cells	130:134	arg1	Cryopreservation					100:115	Cryopreservation	100:115	Cryopreservation of red blood cells (RBCs)	100:141	Cryopreservation of red blood cells (RBCs) is imperative for transfusion therapy, while cryoprotectants are essential to protect RBCs from cryoinjury under freezing temperatures.					
36577138	0	48	with	Integration	0:10	arg1	Trehalose					50:58	Dissociative Trehalose	37:58	Dissociative Trehalose	37:58	Integration of Trehalose Lipids with Dissociative Trehalose Enables Cryopreservation of Human RBCs.					
36577138	3	49	theme	RBCs	516:519	arg1	trehalose					589:597	dissociative trehalose	576:597	dissociative trehalose	576:597	Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization.					
36577138	3	49	theme	RBCs	516:519	arg1	cryopreservation					490:505	glycerol-free cryopreservation	476:505	glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids	476:570	Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization.					
36577138	6	50	theme	membrane-binding	1086:1101	arg1	activity					1103:1110	the moderate membrane-binding activity	1073:1110	the moderate membrane-binding activity of trehalose lipids	1073:1130	It was further confirmed by isothermal titration calorimetry and osmotic fragility tests that the moderate membrane-binding activity of trehalose lipids exerted cell stabilization for high cryosurvival.					
36577138	6	51	dep	calorimetry	1028:1038	arg1	tests					1062:1066	tests	1062:1066	tests	1062:1066	It was further confirmed by isothermal titration calorimetry and osmotic fragility tests that the moderate membrane-binding activity of trehalose lipids exerted cell stabilization for high cryosurvival.					
36577138	4	52	theme	freeze	780:785	arg1	stress					787:792	freeze stress	780:792	freeze stress	780:792	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	6	53	theme	moderate	1077:1084	arg1	activity					1103:1110	the moderate membrane-binding activity	1073:1110	the moderate membrane-binding activity of trehalose lipids	1073:1130	It was further confirmed by isothermal titration calorimetry and osmotic fragility tests that the moderate membrane-binding activity of trehalose lipids exerted cell stabilization for high cryosurvival.					
36577138	4	54	from	able	741:744	arg1	comparison					660:669	comparison	660:669	comparison with sucrose monolaurate or trehalose only	660:712	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	3	55	theme	trehalose	555:563	arg1	lipids					565:570	the synthesized trehalose lipids	539:570	the synthesized trehalose lipids	539:570	Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization.					
36577138	4	56	theme	sucrose	676:682	arg1	monolaurate					684:694	sucrose monolaurate	676:694	sucrose monolaurate	676:694	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	4	57	dep	%	815:815	arg1	±					810:810	±	810:810	±	810:810	Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose.					
36577138	0	58	theme	Trehalose	15:23	arg1	Lipids					25:30	Trehalose Lipids	15:30	Trehalose Lipids	15:30	Integration of Trehalose Lipids with Dissociative Trehalose Enables Cryopreservation of Human RBCs.					
36577138	3	59	theme	lipids	565:570	arg1	integration					524:534	integration	524:534	integration of the synthesized trehalose lipids	524:570	Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization.					
35525452	0	0	theme	blue	90:93	arg1	methylene					80:88	methylene blue	80:93	methylene blue	80:93	Adsorption efficiency of date palm based activated carbon-alginate membrane for methylene blue.					
35525452	4	1	theme	batch	616:620	arg1	investigation					622:634	batch investigation	616:634	batch investigation	616:634	Adsorption of methylene blue dye from aqueous solution was carried out using AC-alg membrane in batch investigation.					
35525452	10	2	theme	MB	1412:1413	arg1	removal					1394:1400	efficient removal	1384:1400	efficient removal of MB and MB like other dyes	1384:1429	The experimental result confirmed that AC-alg membrane is a suitable and easily recoverable adsorbent to be used for efficient removal of MB and MB like other dyes.					
35525452	6	3	theme	dye	923:925	arg1	adsorption					927:936	dye adsorption	923:936	dye adsorption	923:936	Kinetics, isotherm and thermodynamics study was performed for dye adsorption.					
35525452	5	4	theme	MB	697:698	arg1	adsorption					683:692	the adsorption	679:692	the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature	679:806	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	9	5	theme	exothermic	1247:1256	arg1	process					1258:1264	an exothermic process	1244:1264	an exothermic process	1244:1264	Thermodynamic study revealed that the adsorption of MB on AC-alg membrane is spontaneous and an exothermic process.					
35525452	10	6	theme	other	1420:1424	arg1	dyes					1426:1429	other dyes	1420:1429	other dyes	1420:1429	The experimental result confirmed that AC-alg membrane is a suitable and easily recoverable adsorbent to be used for efficient removal of MB and MB like other dyes.					
35525452	5	7	theme	maximum	830:836	arg1	efficiency					849:858	maximum adsorption efficiency	830:858	maximum adsorption efficiency	830:858	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	1	8	theme	activating	220:229	arg1	acid					209:212	boric acid	203:212	boric acid	203:212	In the current study, activated carbon (AC) was prepared from date palm using single step activation using boric acid as an activating agent.					
35525452	1	8	theme	activating	220:229	arg1	agent					231:235	an activating agent	217:235	an activating agent	217:235	In the current study, activated carbon (AC) was prepared from date palm using single step activation using boric acid as an activating agent.					
35525452	10	9	theme	MB	1405:1406	arg1	removal					1394:1400	efficient removal	1384:1400	efficient removal of MB and MB like other dyes	1384:1429	The experimental result confirmed that AC-alg membrane is a suitable and easily recoverable adsorbent to be used for efficient removal of MB and MB like other dyes.					
35525452	9	10	theme	Thermodynamic	1151:1163	arg1	study					1165:1169	Thermodynamic study	1151:1169	Thermodynamic study	1151:1169	Thermodynamic study revealed that the adsorption of MB on AC-alg membrane is spontaneous and an exothermic process.					
35525452	10	11	theme	experimental	1271:1282	arg1	result					1284:1289	The experimental result	1267:1289	The experimental result	1267:1289	The experimental result confirmed that AC-alg membrane is a suitable and easily recoverable adsorbent to be used for efficient removal of MB and MB like other dyes.					
35525452	4	12	from	membrane	604:611	arg1	investigation					622:634	batch investigation	616:634	batch investigation	616:634	Adsorption of methylene blue dye from aqueous solution was carried out using AC-alg membrane in batch investigation.					
35525452	5	13	theme	solution	783:790	arg1	time					756:759	contact time	748:759	contact time	748:759	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	5	13	theme	solution	783:790	arg1	temperature					796:806	temperature	796:806	temperature	796:806	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	5	13	theme	solution	783:790	arg1	pH					728:729	initial pH	720:729	initial pH of dye solution	720:745	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	5	13	theme	solution	783:790	arg1	concentration					762:774	concentration	762:774	concentration of dye solution	762:790	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	8	14	theme	adsorption	1130:1139	arg1	isotherm					1141:1148	Langmuir adsorption isotherm	1121:1148	Langmuir adsorption isotherm	1121:1148	The maximum adsorption capacity for MB adsorption was 666 mg/g found by Langmuir adsorption isotherm.					
35525452	10	15	used	used	1375:1378	arg2	membrane					1313:1320	AC-alg membrane	1306:1320	AC-alg membrane	1306:1320	The experimental result confirmed that AC-alg membrane is a suitable and easily recoverable adsorbent to be used for efficient removal of MB and MB like other dyes.					
35525452	4	16	theme	aqueous	558:564	arg1	solution					566:573	aqueous solution	558:573	aqueous solution	558:573	Adsorption of methylene blue dye from aqueous solution was carried out using AC-alg membrane in batch investigation.					
35525452	2	17	theme	synthesized	242:252	arg1	AC					254:255	The synthesized AC	238:255	The synthesized AC	238:255	The synthesized AC was incorporated with alginate (AC-alginate (AC-alg)) to prepare membrane for adsorption of methylene blue (MB) in batch adsorption study.					
35525452	2	18	theme	methylene	349:357	arg1	adsorption					335:344	adsorption	335:344	adsorption of methylene blue (MB) in batch adsorption study	335:393	The synthesized AC was incorporated with alginate (AC-alginate (AC-alg)) to prepare membrane for adsorption of methylene blue (MB) in batch adsorption study.					
35525452	5	19	theme	dye	734:736	arg1	solution					738:745	dye solution	734:745	dye solution	734:745	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	10	20	theme	AC-alg	1306:1311	arg1	membrane					1313:1320	AC-alg membrane	1306:1320	AC-alg membrane	1306:1320	The experimental result confirmed that AC-alg membrane is a suitable and easily recoverable adsorbent to be used for efficient removal of MB and MB like other dyes.					
35525452	0	21	theme	Adsorption	0:9	arg1	efficiency					11:20	Adsorption efficiency	0:20	Adsorption efficiency of date palm	0:33	Adsorption efficiency of date palm based activated carbon-alginate membrane for methylene blue.					
35525452	5	22	theme	solution	738:745	arg1	time					756:759	contact time	748:759	contact time	748:759	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	5	22	theme	solution	738:745	arg1	temperature					796:806	temperature	796:806	temperature	796:806	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	5	22	theme	solution	738:745	arg1	pH					728:729	initial pH	720:729	initial pH of dye solution	720:745	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	5	22	theme	solution	738:745	arg1	concentration					762:774	concentration	762:774	concentration of dye solution	762:790	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	7	23	theme	experimental	1030:1041	arg1	data					1043:1046	the experimental data	1026:1046	the experimental data	1026:1046	Pseudo-second order kinetic model and Langmuir adsorption isotherm were well fitted to the experimental data.					
35525452	10	24	theme	efficient	1384:1392	arg1	removal					1394:1400	efficient removal	1384:1400	efficient removal of MB and MB like other dyes	1384:1429	The experimental result confirmed that AC-alg membrane is a suitable and easily recoverable adsorbent to be used for efficient removal of MB and MB like other dyes.					
35525452	1	25	theme	date	158:161	arg1	palm					163:166	date palm	158:166	date palm	158:166	In the current study, activated carbon (AC) was prepared from date palm using single step activation using boric acid as an activating agent.					
35525452	2	26	theme	adsorption	378:387	arg1	study					389:393	batch adsorption study	372:393	batch adsorption study	372:393	The synthesized AC was incorporated with alginate (AC-alginate (AC-alg)) to prepare membrane for adsorption of methylene blue (MB) in batch adsorption study.					
35525452	3	27	theme	TGA	506:508	arg1	analysis					510:517	TGA analysis	506:517	TGA analysis	506:517	The prepared membrane was characterized using different types of analytical techniques such as FTIR, SEM, and TGA analysis.					
35525452	4	28	theme	AC-alg	597:602	arg1	membrane					604:611	AC-alg membrane	597:611	AC-alg membrane in batch investigation	597:634	Adsorption of methylene blue dye from aqueous solution was carried out using AC-alg membrane in batch investigation.					
35525452	0	29	theme	date	25:28	arg1	palm					30:33	date palm	25:33	date palm	25:33	Adsorption efficiency of date palm based activated carbon-alginate membrane for methylene blue.					
35525452	8	30	theme	Langmuir	1121:1128	arg1	adsorption					1130:1139	Langmuir adsorption	1121:1139	Langmuir adsorption isotherm	1121:1148	The maximum adsorption capacity for MB adsorption was 666 mg/g found by Langmuir adsorption isotherm.					
35525452	5	31	theme	Various	637:643	arg1	parameters					658:667	Various experimental parameters	637:667	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature	637:806	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	3	32	theme	prepared	400:407	arg1	membrane					409:416	The prepared membrane	396:416	The prepared membrane	396:416	The prepared membrane was characterized using different types of analytical techniques such as FTIR, SEM, and TGA analysis.					
35525452	5	33	theme	experimental	645:656	arg1	parameters					658:667	Various experimental parameters	637:667	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature	637:806	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	9	34	theme	AC-alg	1209:1214	arg1	membrane					1216:1223	AC-alg membrane	1209:1223	AC-alg membrane	1209:1223	Thermodynamic study revealed that the adsorption of MB on AC-alg membrane is spontaneous and an exothermic process.					
35525452	1	35	theme	current	103:109	arg1	study					111:115	the current study	99:115	the current study	99:115	In the current study, activated carbon (AC) was prepared from date palm using single step activation using boric acid as an activating agent.					
35525452	4	36	from	solution	566:573	arg1	Adsorption					520:529	Adsorption	520:529	Adsorption of methylene blue dye from aqueous solution	520:573	Adsorption of methylene blue dye from aqueous solution was carried out using AC-alg membrane in batch investigation.					
35525452	1	37	theme	single	174:179	arg1	activation					186:195	single step activation	174:195	single step activation	174:195	In the current study, activated carbon (AC) was prepared from date palm using single step activation using boric acid as an activating agent.					
35525452	5	38	theme	initial	720:726	arg1	pH					728:729	initial pH	720:729	initial pH of dye solution	720:745	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	9	39	from	adsorption	1189:1198	arg1	membrane					1216:1223	AC-alg membrane	1209:1223	AC-alg membrane	1209:1223	Thermodynamic study revealed that the adsorption of MB on AC-alg membrane is spontaneous and an exothermic process.					
35525452	9	40	theme	MB	1203:1204	arg1	spontaneous					1228:1238	spontaneous	1228:1238	spontaneous	1228:1238	Thermodynamic study revealed that the adsorption of MB on AC-alg membrane is spontaneous and an exothermic process.					
35525452	9	40	theme	MB	1203:1204	arg1	adsorption					1189:1198	the adsorption	1185:1198	the adsorption of MB on AC-alg membrane	1185:1223	Thermodynamic study revealed that the adsorption of MB on AC-alg membrane is spontaneous and an exothermic process.					
35525452	1	41	theme	step	181:184	arg1	activation					186:195	single step activation	174:195	single step activation	174:195	In the current study, activated carbon (AC) was prepared from date palm using single step activation using boric acid as an activating agent.					
35525452	0	42	theme	palm	30:33	arg1	efficiency					11:20	Adsorption efficiency	0:20	Adsorption efficiency of date palm	0:33	Adsorption efficiency of date palm based activated carbon-alginate membrane for methylene blue.					
35525452	6	43	theme	thermodynamics	884:897	arg1	study					899:903	Kinetics, isotherm and thermodynamics study	861:903	Kinetics, isotherm and thermodynamics study	861:903	Kinetics, isotherm and thermodynamics study was performed for dye adsorption.					
35525452	2	44	theme	batch	372:376	arg1	study					389:393	batch adsorption study	372:393	batch adsorption study	372:393	The synthesized AC was incorporated with alginate (AC-alginate (AC-alg)) to prepare membrane for adsorption of methylene blue (MB) in batch adsorption study.					
35525452	2	45	from	adsorption	335:344	arg1	study					389:393	batch adsorption study	372:393	batch adsorption study	372:393	The synthesized AC was incorporated with alginate (AC-alginate (AC-alg)) to prepare membrane for adsorption of methylene blue (MB) in batch adsorption study.					
35525452	2	46	theme	blue	359:362	arg1	methylene					349:357	methylene blue	349:362	methylene blue (MB)	349:367	The synthesized AC was incorporated with alginate (AC-alginate (AC-alg)) to prepare membrane for adsorption of methylene blue (MB) in batch adsorption study.					
35525452	2	46	theme	blue	359:362	arg1	MB					365:366	MB	365:366	MB	365:366	The synthesized AC was incorporated with alginate (AC-alginate (AC-alg)) to prepare membrane for adsorption of methylene blue (MB) in batch adsorption study.					
35525452	0	47	theme	carbon-alginate	51:65	arg1	membrane					67:74	activated carbon-alginate membrane	41:74	activated carbon-alginate membrane	41:74	Adsorption efficiency of date palm based activated carbon-alginate membrane for methylene blue.					
35525452	8	48	theme	adsorption	1061:1070	arg1	capacity					1072:1079	The maximum adsorption capacity	1049:1079	The maximum adsorption capacity for MB adsorption	1049:1097	The maximum adsorption capacity for MB adsorption was 666 mg/g found by Langmuir adsorption isotherm.					
35525452	5	49	from	adsorption	683:692	arg1	membrane					703:710	membrane	703:710	membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature	703:806	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	5	50	theme	adsorption	838:847	arg1	efficiency					849:858	maximum adsorption efficiency	830:858	maximum adsorption efficiency	830:858	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	0	51	theme	activated	41:49	arg1	membrane					67:74	activated carbon-alginate membrane	41:74	activated carbon-alginate membrane	41:74	Adsorption efficiency of date palm based activated carbon-alginate membrane for methylene blue.					
35525452	6	52	theme	isotherm	871:878	arg1	study					899:903	Kinetics, isotherm and thermodynamics study	861:903	Kinetics, isotherm and thermodynamics study	861:903	Kinetics, isotherm and thermodynamics study was performed for dye adsorption.					
35525452	8	53	theme	maximum	1053:1059	arg1	capacity					1072:1079	The maximum adsorption capacity	1049:1079	The maximum adsorption capacity for MB adsorption	1049:1097	The maximum adsorption capacity for MB adsorption was 666 mg/g found by Langmuir adsorption isotherm.					
35525452	5	54	theme	contact	748:754	arg1	time					756:759	contact time	748:759	contact time	748:759	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	7	55	theme	order	953:957	arg1	model					967:971	Pseudo-second order kinetic model	939:971	Pseudo-second order kinetic model	939:971	Pseudo-second order kinetic model and Langmuir adsorption isotherm were well fitted to the experimental data.					
35525452	3	56	theme	different	442:450	arg1	types					452:456	different types	442:456	different types of analytical techniques such as FTIR, SEM, and TGA analysis	442:517	The prepared membrane was characterized using different types of analytical techniques such as FTIR, SEM, and TGA analysis.					
35525452	7	57	theme	Langmuir	977:984	arg1	adsorption					986:995	Langmuir adsorption	977:995	Langmuir adsorption isotherm	977:1004	Pseudo-second order kinetic model and Langmuir adsorption isotherm were well fitted to the experimental data.					
35525452	7	58	theme	kinetic	959:965	arg1	model					967:971	Pseudo-second order kinetic model	939:971	Pseudo-second order kinetic model	939:971	Pseudo-second order kinetic model and Langmuir adsorption isotherm were well fitted to the experimental data.					
35525452	1	59	theme	activated	118:126	arg1	AC					136:137	AC	136:137	AC	136:137	In the current study, activated carbon (AC) was prepared from date palm using single step activation using boric acid as an activating agent.					
35525452	1	59	theme	activated	118:126	arg1	carbon					128:133	activated carbon	118:133	activated carbon (AC)	118:138	In the current study, activated carbon (AC) was prepared from date palm using single step activation using boric acid as an activating agent.					
35525452	4	60	theme	blue	544:547	arg1	dye					549:551	methylene blue dye	534:551	methylene blue dye	534:551	Adsorption of methylene blue dye from aqueous solution was carried out using AC-alg membrane in batch investigation.					
35525452	7	61	theme	adsorption	986:995	arg1	isotherm					997:1004	Langmuir adsorption isotherm	977:1004	Langmuir adsorption isotherm	977:1004	Pseudo-second order kinetic model and Langmuir adsorption isotherm were well fitted to the experimental data.					
35525452	1	62	theme	boric	203:207	arg1	acid					209:212	boric acid	203:212	boric acid	203:212	In the current study, activated carbon (AC) was prepared from date palm using single step activation using boric acid as an activating agent.					
35525452	1	62	theme	boric	203:207	arg1	agent					231:235	an activating agent	217:235	an activating agent	217:235	In the current study, activated carbon (AC) was prepared from date palm using single step activation using boric acid as an activating agent.					
35525452	5	63	theme	dye	779:781	arg1	solution					783:790	dye solution	779:790	dye solution	779:790	Various experimental parameters effecting the adsorption of MB on membrane such as initial pH of dye solution, contact time, concentration of dye solution and temperature were optimized to get maximum adsorption efficiency.					
35525452	7	64	theme	Pseudo-second	939:951	arg1	model					967:971	Pseudo-second order kinetic model	939:971	Pseudo-second order kinetic model	939:971	Pseudo-second order kinetic model and Langmuir adsorption isotherm were well fitted to the experimental data.					
35525452	3	65	theme	analytical	461:470	arg1	FTIR					491:494	FTIR	491:494	FTIR	491:494	The prepared membrane was characterized using different types of analytical techniques such as FTIR, SEM, and TGA analysis.					
35525452	3	65	theme	analytical	461:470	arg1	analysis					510:517	TGA analysis	506:517	TGA analysis	506:517	The prepared membrane was characterized using different types of analytical techniques such as FTIR, SEM, and TGA analysis.					
35525452	3	65	theme	analytical	461:470	arg1	SEM					497:499	SEM	497:499	SEM	497:499	The prepared membrane was characterized using different types of analytical techniques such as FTIR, SEM, and TGA analysis.					
35525452	3	65	theme	analytical	461:470	arg1	techniques					472:481	analytical techniques	461:481	analytical techniques such as FTIR, SEM, and TGA analysis	461:517	The prepared membrane was characterized using different types of analytical techniques such as FTIR, SEM, and TGA analysis.					
35525452	8	66	theme	MB	1085:1086	arg1	adsorption					1088:1097	MB adsorption	1085:1097	MB adsorption	1085:1097	The maximum adsorption capacity for MB adsorption was 666 mg/g found by Langmuir adsorption isotherm.					
35525452	6	67	theme	Kinetics	861:868	arg1	study					899:903	Kinetics, isotherm and thermodynamics study	861:903	Kinetics, isotherm and thermodynamics study	861:903	Kinetics, isotherm and thermodynamics study was performed for dye adsorption.					
35525452	4	68	theme	methylene	534:542	arg1	dye					549:551	methylene blue dye	534:551	methylene blue dye	534:551	Adsorption of methylene blue dye from aqueous solution was carried out using AC-alg membrane in batch investigation.					
35525452	3	69	theme	techniques	472:481	arg1	types					452:456	different types	442:456	different types of analytical techniques such as FTIR, SEM, and TGA analysis	442:517	The prepared membrane was characterized using different types of analytical techniques such as FTIR, SEM, and TGA analysis.					
35525452	4	70	theme	dye	549:551	arg1	Adsorption					520:529	Adsorption	520:529	Adsorption of methylene blue dye from aqueous solution	520:573	Adsorption of methylene blue dye from aqueous solution was carried out using AC-alg membrane in batch investigation.					
36815687	0	0	theme	annulus	92:98	arg1	fibrosus					100:107	combined biomimetic annulus fibrosus	72:107	combined biomimetic annulus fibrosus	72:107	Toward a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs.					
36815687	6	1	theme	novel	911:915	arg1	construct					943:951	a novel biomimetic combined AF-NP construct	909:951	a novel biomimetic combined AF-NP construct	909:951	This study presents three novel substructures-a biomimetic silk-reinforced composite lamella for the AF, a GAG analog for the NP, and a novel biomimetic combined AF-NP construct.					
36815687	10	2	theme	construct	1488:1496	arg1	ability					1467:1473	The ability	1463:1473	The ability of our AF-NP construct to mimic the native IVD	1463:1520	The ability of our AF-NP construct to mimic the native IVD offers a revolutionary concept for the potential development of a fully functional IVD.					
36815687	9	3	theme	finite	1356:1361	arg1	model					1371:1375	a finite element model	1354:1375	a finite element model	1354:1375	Validation of the AF-NP construct mechanics using a finite element model yields results compatible with native human IVD under various physiological loadings.					
36815687	2	4	theme	biomechanical	377:389	arg1	function					391:398	the spine biomechanical function	367:398	the spine biomechanical function	367:398	The IVD complex fibrocartilaginous structure is responsible for the spine biomechanical function.					
36815687	10	5	theme	potential	1561:1569	arg1	development					1571:1581	the potential development	1557:1581	the potential development of a fully functional IVD	1557:1607	The ability of our AF-NP construct to mimic the native IVD offers a revolutionary concept for the potential development of a fully functional IVD.					
36815687	0	6	theme	biomimetic	81:90	arg1	fibrosus					100:107	combined biomimetic annulus fibrosus	72:107	combined biomimetic annulus fibrosus	72:107	Toward a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs.					
36815687	9	7	theme	AF-NP	1322:1326	arg1	mechanics					1338:1346	the AF-NP construct mechanics	1318:1346	the AF-NP construct mechanics using a finite element model	1318:1375	Validation of the AF-NP construct mechanics using a finite element model yields results compatible with native human IVD under various physiological loadings.					
36815687	10	8	theme	AF-NP	1482:1486	arg1	construct					1488:1496	our AF-NP construct	1478:1496	our AF-NP construct	1478:1496	The ability of our AF-NP construct to mimic the native IVD offers a revolutionary concept for the potential development of a fully functional IVD.					
36815687	6	9	theme	composite	850:858	arg1	lamella					860:866	three novel substructures-a biomimetic silk-reinforced composite lamella	795:866	three novel substructures-a biomimetic silk-reinforced composite lamella for the AF	795:877	This study presents three novel substructures-a biomimetic silk-reinforced composite lamella for the AF, a GAG analog for the NP, and a novel biomimetic combined AF-NP construct.					
36815687	2	10	theme	spine	371:375	arg1	function					391:398	the spine biomechanical function	367:398	the spine biomechanical function	367:398	The IVD complex fibrocartilaginous structure is responsible for the spine biomechanical function.					
36815687	8	11	theme	biomimetic	1160:1169	arg1	AF-NP					1171:1175	The synergized biomimetic AF-NP	1145:1175	The synergized biomimetic AF-NP	1145:1175	The synergized biomimetic AF-NP demonstrates similar behavior to the unconfined NP, with significantly increased deformations indicating improved performance.					
36815687	9	12	theme	construct	1328:1336	arg1	mechanics					1338:1346	the AF-NP construct mechanics	1318:1346	the AF-NP construct mechanics using a finite element model	1318:1375	Validation of the AF-NP construct mechanics using a finite element model yields results compatible with native human IVD under various physiological loadings.					
36815687	6	13	theme	silk-reinforced	834:848	arg1	lamella					860:866	three novel substructures-a biomimetic silk-reinforced composite lamella	795:866	three novel substructures-a biomimetic silk-reinforced composite lamella for the AF	795:877	This study presents three novel substructures-a biomimetic silk-reinforced composite lamella for the AF, a GAG analog for the NP, and a novel biomimetic combined AF-NP construct.					
36815687	0	14	theme	fibrosus	100:107	arg1	Engineering					57:67	a mechanically biocompatible intervertebral disc: Engineering	7:67	a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs	7:136	Toward a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs.					
36815687	9	15	theme	mechanics	1338:1346	arg1	Validation					1304:1313	Validation	1304:1313	Validation of the AF-NP construct mechanics using a finite element model	1304:1375	Validation of the AF-NP construct mechanics using a finite element model yields results compatible with native human IVD under various physiological loadings.					
36815687	7	16	theme	similar	1120:1126	arg1	behavior					1111:1118	mechanical behavior	1100:1118	mechanical behavior similar to the human NP	1100:1142	The biomimetic AF demonstrates nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF, while the NP analog demonstrates mechanical behavior similar to the human NP.					
36815687	6	17	theme	biomimetic	823:832	arg1	lamella					860:866	three novel substructures-a biomimetic silk-reinforced composite lamella	795:866	three novel substructures-a biomimetic silk-reinforced composite lamella for the AF	795:877	This study presents three novel substructures-a biomimetic silk-reinforced composite lamella for the AF, a GAG analog for the NP, and a novel biomimetic combined AF-NP construct.					
36815687	3	18	theme	surrounding	510:520	arg1	lamellae					561:568	the surrounding fiber-reinforced annulus fibrosus (AF) lamellae	506:568	the surrounding fiber-reinforced annulus fibrosus (AF) lamellae	506:568	The nucleus pulposus (NP), composed of swellable glycosaminoglycan (GAG), transfers compressive loads to the surrounding fiber-reinforced annulus fibrosus (AF) lamellae, which stretches under tension.					
36815687	1	19	theme	million	277:283	arg1	worldwide					292:300	over 600 million people worldwide	268:300	over 600 million people worldwide	268:300	Intervertebral disc (IVD) degeneration and accompanying lower back pain impose global medical and societal challenges, affecting over 600 million people worldwide.					
36815687	0	20	theme	pulposus	121:128	arg1	analogs					130:136	pulposus analogs	121:136	pulposus analogs	121:136	Toward a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs.					
36815687	10	21	theme	native	1511:1516	arg1	IVD					1518:1520	the native IVD	1507:1520	the native IVD	1507:1520	The ability of our AF-NP construct to mimic the native IVD offers a revolutionary concept for the potential development of a fully functional IVD.					
36815687	6	22	theme	substructures-a	807:821	arg1	lamella					860:866	three novel substructures-a biomimetic silk-reinforced composite lamella	795:866	three novel substructures-a biomimetic silk-reinforced composite lamella for the AF	795:877	This study presents three novel substructures-a biomimetic silk-reinforced composite lamella for the AF, a GAG analog for the NP, and a novel biomimetic combined AF-NP construct.					
36815687	8	23	theme	increased	1248:1256	arg1	deformations					1258:1269	significantly increased deformations	1234:1269	significantly increased deformations indicating improved performance	1234:1301	The synergized biomimetic AF-NP demonstrates similar behavior to the unconfined NP, with significantly increased deformations indicating improved performance.					
36815687	0	24	theme	nucleus	113:119	arg1	Engineering					57:67	a mechanically biocompatible intervertebral disc: Engineering	7:67	a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs	7:136	Toward a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs.					
36815687	7	25	theme	mechanical	1100:1109	arg1	behavior					1111:1118	mechanical behavior	1100:1118	mechanical behavior similar to the human NP	1100:1142	The biomimetic AF demonstrates nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF, while the NP analog demonstrates mechanical behavior similar to the human NP.					
36815687	6	26	theme	novel	801:805	arg1	lamella					860:866	three novel substructures-a biomimetic silk-reinforced composite lamella	795:866	three novel substructures-a biomimetic silk-reinforced composite lamella for the AF	795:877	This study presents three novel substructures-a biomimetic silk-reinforced composite lamella for the AF, a GAG analog for the NP, and a novel biomimetic combined AF-NP construct.					
36815687	0	27	dep	fibrosus	100:107	arg1	analogs					130:136	pulposus analogs	121:136	pulposus analogs	121:136	Toward a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs.					
36815687	4	28	theme	high	669:672	arg1	loads					686:690	extremely high and complex loads	659:690	extremely high and complex loads	659:690	Together, these substructures allow the IVD to withstand extremely high and complex loads.					
36815687	3	29	theme	fibrosus	547:554	arg1	lamellae					561:568	the surrounding fiber-reinforced annulus fibrosus (AF) lamellae	506:568	the surrounding fiber-reinforced annulus fibrosus (AF) lamellae	506:568	The nucleus pulposus (NP), composed of swellable glycosaminoglycan (GAG), transfers compressive loads to the surrounding fiber-reinforced annulus fibrosus (AF) lamellae, which stretches under tension.					
36815687	7	30	theme	NP	1077:1078	arg1	analog					1080:1085	the NP analog	1073:1085	the NP analog	1073:1085	The biomimetic AF demonstrates nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF, while the NP analog demonstrates mechanical behavior similar to the human NP.					
36815687	9	31	theme	element	1363:1369	arg1	model					1371:1375	a finite element model	1354:1375	a finite element model	1354:1375	Validation of the AF-NP construct mechanics using a finite element model yields results compatible with native human IVD under various physiological loadings.					
36815687	7	32	theme	human	1057:1061	arg1	AF					1063:1064	the native human AF	1046:1064	the native human AF	1046:1064	The biomimetic AF demonstrates nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF, while the NP analog demonstrates mechanical behavior similar to the human NP.					
36815687	8	33	theme	similar	1190:1196	arg1	behavior					1198:1205	similar behavior	1190:1205	similar behavior to the unconfined NP	1190:1226	The synergized biomimetic AF-NP demonstrates similar behavior to the unconfined NP, with significantly increased deformations indicating improved performance.					
36815687	1	34	theme	people	285:290	arg1	worldwide					292:300	over 600 million people worldwide	268:300	over 600 million people worldwide	268:300	Intervertebral disc (IVD) degeneration and accompanying lower back pain impose global medical and societal challenges, affecting over 600 million people worldwide.					
36815687	9	35	theme	native	1408:1413	arg1	IVD					1421:1423	native human IVD	1408:1423	native human IVD under various physiological loadings	1408:1460	Validation of the AF-NP construct mechanics using a finite element model yields results compatible with native human IVD under various physiological loadings.					
36815687	2	36	theme	fibrocartilaginous	319:336	arg1	responsible					351:361	responsible	351:361	responsible	351:361	The IVD complex fibrocartilaginous structure is responsible for the spine biomechanical function.					
36815687	2	36	theme	fibrocartilaginous	319:336	arg1	structure					338:346	The IVD complex fibrocartilaginous structure	303:346	The IVD complex fibrocartilaginous structure	303:346	The IVD complex fibrocartilaginous structure is responsible for the spine biomechanical function.					
36815687	9	37	with	compatible	1392:1401	arg1	IVD					1421:1423	native human IVD	1408:1423	native human IVD under various physiological loadings	1408:1460	Validation of the AF-NP construct mechanics using a finite element model yields results compatible with native human IVD under various physiological loadings.					
36815687	3	38	theme	swellable	440:448	arg1	glycosaminoglycan					450:466	swellable glycosaminoglycan	440:466	swellable glycosaminoglycan (GAG)	440:472	The nucleus pulposus (NP), composed of swellable glycosaminoglycan (GAG), transfers compressive loads to the surrounding fiber-reinforced annulus fibrosus (AF) lamellae, which stretches under tension.					
36815687	3	38	theme	swellable	440:448	arg1	GAG					469:471	GAG	469:471	GAG	469:471	The nucleus pulposus (NP), composed of swellable glycosaminoglycan (GAG), transfers compressive loads to the surrounding fiber-reinforced annulus fibrosus (AF) lamellae, which stretches under tension.					
36815687	0	39	theme	biocompatible	22:34	arg1	Engineering					57:67	a mechanically biocompatible intervertebral disc: Engineering	7:67	a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs	7:136	Toward a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs.					
36815687	10	40	theme	revolutionary	1531:1543	arg1	concept					1545:1551	a revolutionary concept	1529:1551	a revolutionary concept for the potential development of a fully functional IVD	1529:1607	The ability of our AF-NP construct to mimic the native IVD offers a revolutionary concept for the potential development of a fully functional IVD.					
36815687	7	41	theme	biomimetic	958:967	arg1	AF					969:970	The biomimetic AF	954:970	The biomimetic AF	954:970	The biomimetic AF demonstrates nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF, while the NP analog demonstrates mechanical behavior similar to the human NP.					
36815687	2	42	theme	complex	311:317	arg1	responsible					351:361	responsible	351:361	responsible	351:361	The IVD complex fibrocartilaginous structure is responsible for the spine biomechanical function.					
36815687	2	42	theme	complex	311:317	arg1	structure					338:346	The IVD complex fibrocartilaginous structure	303:346	The IVD complex fibrocartilaginous structure	303:346	The IVD complex fibrocartilaginous structure is responsible for the spine biomechanical function.					
36815687	9	43	theme	human	1415:1419	arg1	IVD					1421:1423	native human IVD	1408:1423	native human IVD under various physiological loadings	1408:1460	Validation of the AF-NP construct mechanics using a finite element model yields results compatible with native human IVD under various physiological loadings.					
36815687	0	44	theme	mechanically	9:20	arg1	Engineering					57:67	a mechanically biocompatible intervertebral disc: Engineering	7:67	a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs	7:136	Toward a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs.					
36815687	5	45	theme	complete	710:717	arg1	disc					719:722	the complete disc	706:722	the complete disc	706:722	Key to mimic the complete disc must consider the properties of its substructures.					
36815687	2	46	theme	IVD	307:309	arg1	responsible					351:361	responsible	351:361	responsible	351:361	The IVD complex fibrocartilaginous structure is responsible for the spine biomechanical function.					
36815687	2	46	theme	IVD	307:309	arg1	structure					338:346	The IVD complex fibrocartilaginous structure	303:346	The IVD complex fibrocartilaginous structure	303:346	The IVD complex fibrocartilaginous structure is responsible for the spine biomechanical function.					
36815687	8	47	theme	improved	1282:1289	arg1	performance					1291:1301	improved performance	1282:1301	improved performance	1282:1301	The synergized biomimetic AF-NP demonstrates similar behavior to the unconfined NP, with significantly increased deformations indicating improved performance.					
36815687	0	48	theme	disc	51:54	arg1	Engineering					57:67	a mechanically biocompatible intervertebral disc: Engineering	7:67	a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs	7:136	Toward a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs.					
36815687	6	49	theme	GAG	882:884	arg1	analog					886:891	a GAG analog	880:891	a GAG analog for the NP	880:902	This study presents three novel substructures-a biomimetic silk-reinforced composite lamella for the AF, a GAG analog for the NP, and a novel biomimetic combined AF-NP construct.					
36815687	1	50	dep	lower	195:199	arg1	pain					206:209	back pain	201:209	accompanying lower back pain	182:209	Intervertebral disc (IVD) degeneration and accompanying lower back pain impose global medical and societal challenges, affecting over 600 million people worldwide.					
36815687	4	51	theme	complex	678:684	arg1	loads					686:690	extremely high and complex loads	659:690	extremely high and complex loads	659:690	Together, these substructures allow the IVD to withstand extremely high and complex loads.					
36815687	1	52	theme	global	218:223	arg1	challenges					246:255	global medical and societal challenges	218:255	global medical and societal challenges	218:255	Intervertebral disc (IVD) degeneration and accompanying lower back pain impose global medical and societal challenges, affecting over 600 million people worldwide.					
36815687	0	53	theme	intervertebral	36:49	arg1	Engineering					57:67	a mechanically biocompatible intervertebral disc: Engineering	7:67	a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs	7:136	Toward a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs.					
36815687	9	54	theme	compatible	1392:1401	arg1	results					1384:1390	results	1384:1390	results compatible with native human IVD under various physiological loadings	1384:1460	Validation of the AF-NP construct mechanics using a finite element model yields results compatible with native human IVD under various physiological loadings.					
36815687	8	55	theme	unconfined	1214:1223	arg1	NP					1225:1226	the unconfined NP	1210:1226	the unconfined NP	1210:1226	The synergized biomimetic AF-NP demonstrates similar behavior to the unconfined NP, with significantly increased deformations indicating improved performance.					
36815687	1	56	theme	medical	225:231	arg1	challenges					246:255	global medical and societal challenges	218:255	global medical and societal challenges	218:255	Intervertebral disc (IVD) degeneration and accompanying lower back pain impose global medical and societal challenges, affecting over 600 million people worldwide.					
36815687	7	57	theme	human	1135:1139	arg1	NP					1141:1142	the human NP	1131:1142	the human NP	1131:1142	The biomimetic AF demonstrates nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF, while the NP analog demonstrates mechanical behavior similar to the human NP.					
36815687	7	58	theme	native	1050:1055	arg1	AF					1063:1064	the native human AF	1046:1064	the native human AF	1046:1064	The biomimetic AF demonstrates nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF, while the NP analog demonstrates mechanical behavior similar to the human NP.					
36815687	7	59	theme	similar	1035:1041	arg1	behavior					1026:1033	nonlinear, hyperelastic, and anisotropic behavior	985:1033	nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF	985:1064	The biomimetic AF demonstrates nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF, while the NP analog demonstrates mechanical behavior similar to the human NP.					
36815687	6	60	theme	AF-NP	937:941	arg1	construct					943:951	a novel biomimetic combined AF-NP construct	909:951	a novel biomimetic combined AF-NP construct	909:951	This study presents three novel substructures-a biomimetic silk-reinforced composite lamella for the AF, a GAG analog for the NP, and a novel biomimetic combined AF-NP construct.					
36815687	3	61	theme	compressive	485:495	arg1	loads					497:501	compressive loads	485:501	compressive loads	485:501	The nucleus pulposus (NP), composed of swellable glycosaminoglycan (GAG), transfers compressive loads to the surrounding fiber-reinforced annulus fibrosus (AF) lamellae, which stretches under tension.					
36815687	3	62	theme	fiber-reinforced	522:537	arg1	lamellae					561:568	the surrounding fiber-reinforced annulus fibrosus (AF) lamellae	506:568	the surrounding fiber-reinforced annulus fibrosus (AF) lamellae	506:568	The nucleus pulposus (NP), composed of swellable glycosaminoglycan (GAG), transfers compressive loads to the surrounding fiber-reinforced annulus fibrosus (AF) lamellae, which stretches under tension.					
36815687	10	63	theme	functional	1594:1603	arg1	IVD					1605:1607	a fully functional IVD	1586:1607	a fully functional IVD	1586:1607	The ability of our AF-NP construct to mimic the native IVD offers a revolutionary concept for the potential development of a fully functional IVD.					
36815687	6	64	theme	combined	928:935	arg1	construct					943:951	a novel biomimetic combined AF-NP construct	909:951	a novel biomimetic combined AF-NP construct	909:951	This study presents three novel substructures-a biomimetic silk-reinforced composite lamella for the AF, a GAG analog for the NP, and a novel biomimetic combined AF-NP construct.					
36815687	7	65	theme	anisotropic	1014:1024	arg1	behavior					1026:1033	nonlinear, hyperelastic, and anisotropic behavior	985:1033	nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF	985:1064	The biomimetic AF demonstrates nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF, while the NP analog demonstrates mechanical behavior similar to the human NP.					
36815687	1	66	theme	Intervertebral	139:152	arg1	degeneration					165:176	Intervertebral disc (IVD) degeneration	139:176	Intervertebral disc (IVD) degeneration	139:176	Intervertebral disc (IVD) degeneration and accompanying lower back pain impose global medical and societal challenges, affecting over 600 million people worldwide.					
36815687	10	67	theme	IVD	1605:1607	arg1	development					1571:1581	the potential development	1557:1581	the potential development of a fully functional IVD	1557:1607	The ability of our AF-NP construct to mimic the native IVD offers a revolutionary concept for the potential development of a fully functional IVD.					
36815687	3	68	theme	AF	557:558	arg1	lamellae					561:568	the surrounding fiber-reinforced annulus fibrosus (AF) lamellae	506:568	the surrounding fiber-reinforced annulus fibrosus (AF) lamellae	506:568	The nucleus pulposus (NP), composed of swellable glycosaminoglycan (GAG), transfers compressive loads to the surrounding fiber-reinforced annulus fibrosus (AF) lamellae, which stretches under tension.					
36815687	7	69	theme	hyperelastic	996:1007	arg1	behavior					1026:1033	nonlinear, hyperelastic, and anisotropic behavior	985:1033	nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF	985:1064	The biomimetic AF demonstrates nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF, while the NP analog demonstrates mechanical behavior similar to the human NP.					
36815687	1	70	theme	disc	154:157	arg1	degeneration					165:176	Intervertebral disc (IVD) degeneration	139:176	Intervertebral disc (IVD) degeneration	139:176	Intervertebral disc (IVD) degeneration and accompanying lower back pain impose global medical and societal challenges, affecting over 600 million people worldwide.					
36815687	1	71	theme	societal	237:244	arg1	challenges					246:255	global medical and societal challenges	218:255	global medical and societal challenges	218:255	Intervertebral disc (IVD) degeneration and accompanying lower back pain impose global medical and societal challenges, affecting over 600 million people worldwide.					
36815687	0	72	theme	combined	72:79	arg1	fibrosus					100:107	combined biomimetic annulus fibrosus	72:107	combined biomimetic annulus fibrosus	72:107	Toward a mechanically biocompatible intervertebral disc: Engineering of combined biomimetic annulus fibrosus and nucleus pulposus analogs.					
36815687	3	73	theme	annulus	539:545	arg1	lamellae					561:568	the surrounding fiber-reinforced annulus fibrosus (AF) lamellae	506:568	the surrounding fiber-reinforced annulus fibrosus (AF) lamellae	506:568	The nucleus pulposus (NP), composed of swellable glycosaminoglycan (GAG), transfers compressive loads to the surrounding fiber-reinforced annulus fibrosus (AF) lamellae, which stretches under tension.					
36815687	9	74	theme	various	1431:1437	arg1	loadings					1453:1460	various physiological loadings	1431:1460	various physiological loadings	1431:1460	Validation of the AF-NP construct mechanics using a finite element model yields results compatible with native human IVD under various physiological loadings.					
36815687	5	75	theme	substructures	760:772	arg1	properties					742:751	the properties	738:751	the properties of its substructures	738:772	Key to mimic the complete disc must consider the properties of its substructures.					
36815687	6	76	theme	biomimetic	917:926	arg1	construct					943:951	a novel biomimetic combined AF-NP construct	909:951	a novel biomimetic combined AF-NP construct	909:951	This study presents three novel substructures-a biomimetic silk-reinforced composite lamella for the AF, a GAG analog for the NP, and a novel biomimetic combined AF-NP construct.					
36815687	3	77	theme	nucleus	405:411	arg1	pulposus					413:420	The nucleus pulposus	401:420	The nucleus pulposus (NP)	401:425	The nucleus pulposus (NP), composed of swellable glycosaminoglycan (GAG), transfers compressive loads to the surrounding fiber-reinforced annulus fibrosus (AF) lamellae, which stretches under tension.					
36815687	3	77	theme	nucleus	405:411	arg1	NP					423:424	NP	423:424	NP	423:424	The nucleus pulposus (NP), composed of swellable glycosaminoglycan (GAG), transfers compressive loads to the surrounding fiber-reinforced annulus fibrosus (AF) lamellae, which stretches under tension.					
36815687	7	78	theme	nonlinear	985:993	arg1	behavior					1026:1033	nonlinear, hyperelastic, and anisotropic behavior	985:1033	nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF	985:1064	The biomimetic AF demonstrates nonlinear, hyperelastic, and anisotropic behavior similar to the native human AF, while the NP analog demonstrates mechanical behavior similar to the human NP.					
36815687	1	79	theme	IVD	160:162	arg1	degeneration					165:176	Intervertebral disc (IVD) degeneration	139:176	Intervertebral disc (IVD) degeneration	139:176	Intervertebral disc (IVD) degeneration and accompanying lower back pain impose global medical and societal challenges, affecting over 600 million people worldwide.					
36815687	9	80	theme	physiological	1439:1451	arg1	loadings					1453:1460	various physiological loadings	1431:1460	various physiological loadings	1431:1460	Validation of the AF-NP construct mechanics using a finite element model yields results compatible with native human IVD under various physiological loadings.					
36815687	8	81	theme	synergized	1149:1158	arg1	AF-NP					1171:1175	The synergized biomimetic AF-NP	1145:1175	The synergized biomimetic AF-NP	1145:1175	The synergized biomimetic AF-NP demonstrates similar behavior to the unconfined NP, with significantly increased deformations indicating improved performance.					
35772268	6	0	from	degradation	978:988	arg1	system					1019:1024	this coupled system	1006:1024	this coupled system	1006:1024	According to the analysis of intermediates, the degradation of 1,2,3-TCB in this coupled system involved stepwise dechlorination and ring opening.					
35772268	0	1	theme	1,2,3-	89:94	arg1	degradation					74:84	degradation	74:84	degradation of 1,2,3- and 1,3,5-trichlorobenzene	74:121	Intimately coupled photocatalysis and functional bacterial system enhance degradation of 1,2,3- and 1,3,5-trichlorobenzene.					
35772268	2	2	theme	biological	376:385	arg1	source					387:392	a biological source	374:392	a biological source	374:392	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	2	2	theme	biological	376:385	arg1	Trichoderma					296:306	Trichoderma	296:306	Trichoderma with benzene degradation ability coupled with activated sludge	296:369	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	6	3	theme	ring	1063:1066	arg1	opening					1068:1074	ring opening	1063:1074	ring opening	1063:1074	According to the analysis of intermediates, the degradation of 1,2,3-TCB in this coupled system involved stepwise dechlorination and ring opening.					
35772268	7	4	theme	aromatic	1270:1277	arg1	hydrocarbon					1279:1289	biodegradable aromatic hydrocarbon	1256:1289	biodegradable aromatic hydrocarbon	1256:1289	The biofilm in ICPB carrier evolved to be enriched in Cutaneotrichosporon, Trichoderma, Apiotrichum, Zoogloea, Dechloromonas, Flavihumibacter and Cupriavidus, which are known for biodegradable aromatic hydrocarbon and halogenate.					
35772268	6	5	theme	intermediates	959:971	arg1	analysis					947:954	the analysis	943:954	the analysis of intermediates	943:971	According to the analysis of intermediates, the degradation of 1,2,3-TCB in this coupled system involved stepwise dechlorination and ring opening.					
35772268	2	6	theme	benzene	313:319	arg1	ability					333:339	benzene degradation ability	313:339	benzene degradation ability coupled with activated sludge	313:369	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	0	7	theme	1,3,5-trichlorobenzene	100:121	arg1	degradation					74:84	degradation	74:84	degradation of 1,2,3- and 1,3,5-trichlorobenzene	74:121	Intimately coupled photocatalysis and functional bacterial system enhance degradation of 1,2,3- and 1,3,5-trichlorobenzene.					
35772268	2	8	theme	activated	354:362	arg1	sludge					364:369	activated sludge	354:369	activated sludge	354:369	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	5	9	theme	ICPB	819:822	arg1	carriers					824:831	the ICPB carriers	815:831	the ICPB carriers	815:831	The biofilms inside the ICPB carriers can mineralize photocatalytic products, which increases the mineralization efficiency by 18.74%.					
35772268	2	10	with	Trichoderma	296:306	arg1	ability					333:339	benzene degradation ability	313:339	benzene degradation ability coupled with activated sludge	313:369	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	8	11	theme	Trichoderma-based	1347:1363	arg1	ICPB					1365:1368	Trichoderma-based ICPB	1347:1368	Trichoderma-based ICPB	1347:1368	Novel microbial seeds supplemented with Trichoderma-based ICPB seem to provide a new potential strategy for effective degradation and mineralization of TCB.					
35772268	8	12	theme	TCB	1459:1461	arg1	mineralization					1441:1454	mineralization	1441:1454	mineralization of TCB	1441:1461	Novel microbial seeds supplemented with Trichoderma-based ICPB seem to provide a new potential strategy for effective degradation and mineralization of TCB.					
35772268	8	12	theme	TCB	1459:1461	arg1	degradation					1425:1435	effective degradation	1415:1435	effective degradation	1415:1435	Novel microbial seeds supplemented with Trichoderma-based ICPB seem to provide a new potential strategy for effective degradation and mineralization of TCB.					
35772268	2	13	theme	cellulose	416:424	arg1	composite					426:434	sugarcane bagasse cellulose composite	398:434	sugarcane bagasse cellulose composite	398:434	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	7	14	theme	biodegradable	1256:1268	arg1	hydrocarbon					1279:1289	biodegradable aromatic hydrocarbon	1256:1289	biodegradable aromatic hydrocarbon	1256:1289	The biofilm in ICPB carrier evolved to be enriched in Cutaneotrichosporon, Trichoderma, Apiotrichum, Zoogloea, Dechloromonas, Flavihumibacter and Cupriavidus, which are known for biodegradable aromatic hydrocarbon and halogenate.					
35772268	4	15	theme	1,3,5-TCB	716:724	arg1	efficiency					688:697	the degradation efficiency	672:697	the degradation efficiency of 1,2,3-TCB and 1,3,5-TCB	672:724	ICPB increased the degradation efficiency of 1,2,3-TCB and 1,3,5-TCB by 12.43% and 4.67%, respectively, compared to photocatalysis alone.					
35772268	0	16	theme	coupled	11:17	arg1	photocatalysis					19:32	Intimately coupled photocatalysis	0:32	Intimately coupled photocatalysis	0:32	Intimately coupled photocatalysis and functional bacterial system enhance degradation of 1,2,3- and 1,3,5-trichlorobenzene.					
35772268	2	17	theme	trichlorobenzene	517:532	arg1	mineralization					499:512	mineralization	499:512	mineralization of trichlorobenzene	499:532	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	2	17	theme	trichlorobenzene	517:532	arg1	degradation					483:493	excellent degradation	473:493	excellent degradation	473:493	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	1	18	theme	promising	202:210	arg1	approach					212:219	a promising approach	200:219	a promising approach for the degradation of recalcitrant organic compounds	200:273	Intimate coupling of photocatalysis and biodegradation (ICPB) is considered a promising approach for the degradation of recalcitrant organic compounds.					
35772268	7	19	dep	Zoogloea	1178:1185	arg1	Apiotrichum					1165:1175	Apiotrichum	1165:1175	Apiotrichum	1165:1175	The biofilm in ICPB carrier evolved to be enriched in Cutaneotrichosporon, Trichoderma, Apiotrichum, Zoogloea, Dechloromonas, Flavihumibacter and Cupriavidus, which are known for biodegradable aromatic hydrocarbon and halogenate.					
35772268	6	20	theme	stepwise	1035:1042	arg1	dechlorination					1044:1057	stepwise dechlorination	1035:1057	stepwise dechlorination	1035:1057	According to the analysis of intermediates, the degradation of 1,2,3-TCB in this coupled system involved stepwise dechlorination and ring opening.					
35772268	2	21	theme	bagasse	408:414	arg1	composite					426:434	sugarcane bagasse cellulose composite	398:434	sugarcane bagasse cellulose composite	398:434	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	8	22	theme	new	1388:1390	arg1	strategy					1402:1409	a new potential strategy	1386:1409	a new potential strategy for effective degradation and mineralization of TCB	1386:1461	Novel microbial seeds supplemented with Trichoderma-based ICPB seem to provide a new potential strategy for effective degradation and mineralization of TCB.					
35772268	1	23	theme	Intimate	124:131	arg1	coupling					133:140	Intimate coupling	124:140	Intimate coupling of photocatalysis and biodegradation (ICPB)	124:184	Intimate coupling of photocatalysis and biodegradation (ICPB) is considered a promising approach for the degradation of recalcitrant organic compounds.					
35772268	2	24	theme	ICPB	454:457	arg1	system					459:464	the ICPB system	450:464	the ICPB system	450:464	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	2	25	theme	degradation	321:331	arg1	ability					333:339	benzene degradation ability	313:339	benzene degradation ability coupled with activated sludge	313:369	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	5	26	theme	mineralization	893:906	arg1	efficiency					908:917	the mineralization efficiency	889:917	the mineralization efficiency	889:917	The biofilms inside the ICPB carriers can mineralize photocatalytic products, which increases the mineralization efficiency by 18.74%.					
35772268	0	27	theme	bacterial	49:57	arg1	system					59:64	functional bacterial system	38:64	functional bacterial system	38:64	Intimately coupled photocatalysis and functional bacterial system enhance degradation of 1,2,3- and 1,3,5-trichlorobenzene.					
35772268	6	28	theme	coupled	1011:1017	arg1	system					1019:1024	this coupled system	1006:1024	this coupled system	1006:1024	According to the analysis of intermediates, the degradation of 1,2,3-TCB in this coupled system involved stepwise dechlorination and ring opening.					
35772268	7	29	from	biofilm	1081:1087	arg1	carrier					1097:1103	ICPB carrier	1092:1103	ICPB carrier	1092:1103	The biofilm in ICPB carrier evolved to be enriched in Cutaneotrichosporon, Trichoderma, Apiotrichum, Zoogloea, Dechloromonas, Flavihumibacter and Cupriavidus, which are known for biodegradable aromatic hydrocarbon and halogenate.					
35772268	0	30	theme	functional	38:47	arg1	system					59:64	functional bacterial system	38:64	functional bacterial system	38:64	Intimately coupled photocatalysis and functional bacterial system enhance degradation of 1,2,3- and 1,3,5-trichlorobenzene.					
35772268	4	31	theme	degradation	676:686	arg1	efficiency					688:697	the degradation efficiency	672:697	the degradation efficiency of 1,2,3-TCB and 1,3,5-TCB	672:724	ICPB increased the degradation efficiency of 1,2,3-TCB and 1,3,5-TCB by 12.43% and 4.67%, respectively, compared to photocatalysis alone.					
35772268	4	32	theme	1,2,3-TCB	702:710	arg1	efficiency					688:697	the degradation efficiency	672:697	the degradation efficiency of 1,2,3-TCB and 1,3,5-TCB	672:724	ICPB increased the degradation efficiency of 1,2,3-TCB and 1,3,5-TCB by 12.43% and 4.67%, respectively, compared to photocatalysis alone.					
35772268	8	33	theme	Novel	1307:1311	arg1	seeds					1323:1327	Novel microbial seeds	1307:1327	Novel microbial seeds supplemented with Trichoderma-based ICPB	1307:1368	Novel microbial seeds supplemented with Trichoderma-based ICPB seem to provide a new potential strategy for effective degradation and mineralization of TCB.					
35772268	5	34	theme	photocatalytic	848:861	arg1	products					863:870	photocatalytic products	848:870	photocatalytic products	848:870	The biofilms inside the ICPB carriers can mineralize photocatalytic products, which increases the mineralization efficiency by 18.74%.					
35772268	6	35	theme	1,2,3-TCB	993:1001	arg1	degradation					978:988	the degradation	974:988	the degradation of 1,2,3-TCB in this coupled system	974:1024	According to the analysis of intermediates, the degradation of 1,2,3-TCB in this coupled system involved stepwise dechlorination and ring opening.					
35772268	8	36	theme	effective	1415:1423	arg1	degradation					1425:1435	effective degradation	1415:1435	effective degradation	1415:1435	Novel microbial seeds supplemented with Trichoderma-based ICPB seem to provide a new potential strategy for effective degradation and mineralization of TCB.					
35772268	3	37	theme	photocatalytic	632:645	arg1	products					647:654	the photocatalytic products	628:654	the photocatalytic products	628:654	The biofilm inside the ICPB carrier can degrade and mineralize the photocatalytic products.					
35772268	1	38	theme	photocatalysis	145:158	arg1	coupling					133:140	Intimate coupling	124:140	Intimate coupling of photocatalysis and biodegradation (ICPB)	124:184	Intimate coupling of photocatalysis and biodegradation (ICPB) is considered a promising approach for the degradation of recalcitrant organic compounds.					
35772268	7	39	theme	ICPB	1092:1095	arg1	carrier					1097:1103	ICPB carrier	1092:1103	ICPB carrier	1092:1103	The biofilm in ICPB carrier evolved to be enriched in Cutaneotrichosporon, Trichoderma, Apiotrichum, Zoogloea, Dechloromonas, Flavihumibacter and Cupriavidus, which are known for biodegradable aromatic hydrocarbon and halogenate.					
35772268	8	40	theme	potential	1392:1400	arg1	strategy					1402:1409	a new potential strategy	1386:1409	a new potential strategy for effective degradation and mineralization of TCB	1386:1461	Novel microbial seeds supplemented with Trichoderma-based ICPB seem to provide a new potential strategy for effective degradation and mineralization of TCB.					
35772268	1	41	theme	recalcitrant	244:255	arg1	compounds					265:273	recalcitrant organic compounds	244:273	recalcitrant organic compounds	244:273	Intimate coupling of photocatalysis and biodegradation (ICPB) is considered a promising approach for the degradation of recalcitrant organic compounds.					
35772268	3	42	theme	ICPB	588:591	arg1	carrier					593:599	the ICPB carrier	584:599	the ICPB carrier	584:599	The biofilm inside the ICPB carrier can degrade and mineralize the photocatalytic products.					
35772268	1	43	theme	biodegradation	164:177	arg1	coupling					133:140	Intimate coupling	124:140	Intimate coupling of photocatalysis and biodegradation (ICPB)	124:184	Intimate coupling of photocatalysis and biodegradation (ICPB) is considered a promising approach for the degradation of recalcitrant organic compounds.					
35772268	1	44	theme	organic	257:263	arg1	compounds					265:273	recalcitrant organic compounds	244:273	recalcitrant organic compounds	244:273	Intimate coupling of photocatalysis and biodegradation (ICPB) is considered a promising approach for the degradation of recalcitrant organic compounds.					
35772268	2	45	theme	light	548:552	arg1	induction					554:562	visible light induction	540:562	visible light induction	540:562	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	2	46	theme	sugarcane	398:406	arg1	composite					426:434	sugarcane bagasse cellulose composite	398:434	sugarcane bagasse cellulose composite	398:434	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	8	47	theme	microbial	1313:1321	arg1	seeds					1323:1327	Novel microbial seeds	1307:1327	Novel microbial seeds supplemented with Trichoderma-based ICPB	1307:1368	Novel microbial seeds supplemented with Trichoderma-based ICPB seem to provide a new potential strategy for effective degradation and mineralization of TCB.					
35772268	1	48	theme	compounds	265:273	arg1	degradation					229:239	the degradation	225:239	the degradation of recalcitrant organic compounds	225:273	Intimate coupling of photocatalysis and biodegradation (ICPB) is considered a promising approach for the degradation of recalcitrant organic compounds.					
35772268	2	49	theme	visible	540:546	arg1	induction					554:562	visible light induction	540:562	visible light induction	540:562	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35772268	2	50	theme	excellent	473:481	arg1	degradation					483:493	excellent degradation	473:493	excellent degradation	473:493	In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction.					
35128552	0	0	theme	strain	93:98	arg1	hydrogels					71:79	composite hydrogels	61:79	composite hydrogels	61:79	Stretchable, self-healing and adhesive sodium alginate-based composite hydrogels as wearable strain sensors for expansion-contraction motion monitoring.					
35128552	0	0	theme	strain	93:98	arg1	sensors					100:106	wearable strain sensors	84:106	wearable strain sensors for expansion-contraction motion monitoring	84:150	Stretchable, self-healing and adhesive sodium alginate-based composite hydrogels as wearable strain sensors for expansion-contraction motion monitoring.					
35128552	6	1	theme	subtle	1360:1365	arg1	motions					1389:1395	subtle expansion-contraction motions	1360:1395	subtle expansion-contraction motions (e.g., human breathing and mouse heart beating)	1360:1443	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	2	2	theme	present	398:404	arg1	detectors					413:421	the present motion detectors	394:421	the present motion detectors	394:421	However, the present motion detectors mainly focus on stretching, bending, and twisting of different body parts while the expansion-contraction motion has been rarely investigated.					
35128552	1	3	theme	human	297:301	arg1	motion					303:308	human motion	297:308	human motion	297:308	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired.					
35128552	0	4	theme	wearable	84:91	arg1	hydrogels					71:79	composite hydrogels	61:79	composite hydrogels	61:79	Stretchable, self-healing and adhesive sodium alginate-based composite hydrogels as wearable strain sensors for expansion-contraction motion monitoring.					
35128552	0	4	theme	wearable	84:91	arg1	sensors					100:106	wearable strain sensors	84:106	wearable strain sensors for expansion-contraction motion monitoring	84:150	Stretchable, self-healing and adhesive sodium alginate-based composite hydrogels as wearable strain sensors for expansion-contraction motion monitoring.					
35128552	7	5	from	applications	1624:1635	arg1	monitoring					1656:1665	personal health monitoring	1640:1665	personal health monitoring	1640:1665	Therefore, this multifunctional hydrogel is highly suitable for monitoring expansion-contraction motions, indicating its potential applications in personal health monitoring.					
35128552	7	6	theme	expansion-contraction	1568:1588	arg1	motions					1590:1596	expansion-contraction motions	1568:1596	expansion-contraction motions	1568:1596	Therefore, this multifunctional hydrogel is highly suitable for monitoring expansion-contraction motions, indicating its potential applications in personal health monitoring.					
35128552	1	7	with	hydrogels	180:188	arg1	conductivity					251:262	conductivity	251:262	conductivity	251:262	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired.					
35128552	1	7	with	hydrogels	180:188	arg1	adhesiveness					233:244	adhesiveness	233:244	adhesiveness	233:244	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired.					
35128552	1	7	with	hydrogels	180:188	arg1	stretchability					195:208	stretchability	195:208	stretchability	195:208	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired.					
35128552	1	7	with	hydrogels	180:188	arg1	ability					224:230	self-healing ability	211:230	self-healing ability	211:230	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired.					
35128552	6	8	theme	beating	1436:1442	arg1	heart					1430:1434	mouse heart beating	1424:1442	mouse heart beating	1424:1442	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	5	9	theme	satisfactory	1102:1113	arg1	stretchability					1115:1128	a satisfactory stretchability	1100:1128	a satisfactory stretchability (500%)	1100:1135	Moreover, the as-prepared hydrogel displayed a satisfactory stretchability (500%) and self-adhesiveness to various substrates.					
35128552	5	9	theme	satisfactory	1102:1113	arg1	%					1134:1134	500%	1131:1134	500%	1131:1134	Moreover, the as-prepared hydrogel displayed a satisfactory stretchability (500%) and self-adhesiveness to various substrates.					
35128552	1	10	theme	health	314:319	arg1	monitoring					321:330	health monitoring	314:330	health monitoring	314:330	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired.					
35128552	0	11	theme	motion	134:139	arg1	monitoring					141:150	expansion-contraction motion monitoring	112:150	expansion-contraction motion monitoring	112:150	Stretchable, self-healing and adhesive sodium alginate-based composite hydrogels as wearable strain sensors for expansion-contraction motion monitoring.					
35128552	1	12	theme	self-healing	211:222	arg1	ability					224:230	self-healing ability	211:230	self-healing ability	211:230	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired.					
35128552	0	13	theme	expansion-contraction	112:132	arg1	monitoring					141:150	expansion-contraction motion monitoring	112:150	expansion-contraction motion monitoring	112:150	Stretchable, self-healing and adhesive sodium alginate-based composite hydrogels as wearable strain sensors for expansion-contraction motion monitoring.					
35128552	6	14	theme	human	1404:1408	arg1	breathing					1410:1418	human breathing	1404:1418	human breathing	1404:1418	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	0	15	theme	Stretchable	0:10	arg1	sodium					39:44	Stretchable, self-healing and adhesive sodium	0:44	Stretchable, self-healing and adhesive sodium	0:44	Stretchable, self-healing and adhesive sodium alginate-based composite hydrogels as wearable strain sensors for expansion-contraction motion monitoring.					
35128552	2	16	theme	body	486:489	arg1	parts					491:495	different body parts	476:495	different body parts	476:495	However, the present motion detectors mainly focus on stretching, bending, and twisting of different body parts while the expansion-contraction motion has been rarely investigated.					
35128552	6	17	theme	fast	1250:1253	arg1	response					1255:1262	a fast response	1248:1262	a fast response (150 ms)	1248:1271	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	6	17	theme	fast	1250:1253	arg1	ms					1269:1270	150 ms	1265:1270	150 ms	1265:1270	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	2	18	theme	different	476:484	arg1	parts					491:495	different body parts	476:495	different body parts	476:495	However, the present motion detectors mainly focus on stretching, bending, and twisting of different body parts while the expansion-contraction motion has been rarely investigated.					
35128552	4	19	theme	hydrogel	1045:1052	arg1	strength					1001:1008	the mechanical strength	986:1008	the mechanical strength	986:1008	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	4	19	theme	hydrogel	1045:1052	arg1	conductivity					1025:1036	electrical conductivity	1014:1036	electrical conductivity	1014:1036	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	6	20	theme	Alg-CNT	1216:1222	arg1	capable					1338:1344	capable	1338:1344	capable	1338:1344	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	6	20	theme	Alg-CNT	1216:1222	arg1	hydrogel					1224:1231	the Alg-CNT hydrogel	1212:1231	the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles)	1212:1313	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	3	21	theme	carbon	592:597	arg1	nanotubes					599:607	carbon nanotubes	592:607	carbon nanotubes (CNTs) as conductive components	592:639	In this study, along with carbon nanotubes (CNTs) as conductive components, sodium alginate (Alg) modified with 3-aminophenylboronic acid (PBA) and dopamine (DA) were synthesized and employed as precursors to prepare a multifunctional Alg-CNT hydrogel.					
35128552	3	21	theme	carbon	592:597	arg1	CNTs					610:613	CNTs	610:613	CNTs	610:613	In this study, along with carbon nanotubes (CNTs) as conductive components, sodium alginate (Alg) modified with 3-aminophenylboronic acid (PBA) and dopamine (DA) were synthesized and employed as precursors to prepare a multifunctional Alg-CNT hydrogel.					
35128552	4	22	theme	dynamic	830:836	arg1	bonds					847:851	dynamic covalent bonds	830:851	dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s)	830:933	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	0	23	theme	self-healing	13:24	arg1	sodium					39:44	Stretchable, self-healing and adhesive sodium	0:44	Stretchable, self-healing and adhesive sodium	0:44	Stretchable, self-healing and adhesive sodium alginate-based composite hydrogels as wearable strain sensors for expansion-contraction motion monitoring.					
35128552	7	24	theme	health	1649:1654	arg1	monitoring					1656:1665	personal health monitoring	1640:1665	personal health monitoring	1640:1665	Therefore, this multifunctional hydrogel is highly suitable for monitoring expansion-contraction motions, indicating its potential applications in personal health monitoring.					
35128552	5	25	theme	as-prepared	1069:1079	arg1	hydrogel					1081:1088	the as-prepared hydrogel	1065:1088	the as-prepared hydrogel	1065:1088	Moreover, the as-prepared hydrogel displayed a satisfactory stretchability (500%) and self-adhesiveness to various substrates.					
35128552	2	26	theme	expansion-contraction	507:527	arg1	motion					529:534	the expansion-contraction motion	503:534	the expansion-contraction motion	503:534	However, the present motion detectors mainly focus on stretching, bending, and twisting of different body parts while the expansion-contraction motion has been rarely investigated.					
35128552	1	27	theme	great	346:350	arg1	attention					352:360	great attention	346:360	great attention	346:360	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired.					
35128552	3	28	theme	conductive	619:628	arg1	components					630:639	conductive components	619:639	conductive components	619:639	In this study, along with carbon nanotubes (CNTs) as conductive components, sodium alginate (Alg) modified with 3-aminophenylboronic acid (PBA) and dopamine (DA) were synthesized and employed as precursors to prepare a multifunctional Alg-CNT hydrogel.					
35128552	0	29	theme	adhesive	30:37	arg1	sodium					39:44	Stretchable, self-healing and adhesive sodium	0:44	Stretchable, self-healing and adhesive sodium	0:44	Stretchable, self-healing and adhesive sodium alginate-based composite hydrogels as wearable strain sensors for expansion-contraction motion monitoring.					
35128552	6	30	theme	expansion-contraction	1367:1387	arg1	motions					1389:1395	subtle expansion-contraction motions	1360:1395	subtle expansion-contraction motions (e.g., human breathing and mouse heart beating)	1360:1443	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	4	31	theme	mechanical	990:999	arg1	strength					1001:1008	the mechanical strength	986:1008	the mechanical strength	986:1008	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	7	32	theme	multifunctional	1509:1523	arg1	hydrogel					1525:1532	this multifunctional hydrogel	1504:1532	this multifunctional hydrogel	1504:1532	Therefore, this multifunctional hydrogel is highly suitable for monitoring expansion-contraction motions, indicating its potential applications in personal health monitoring.					
35128552	4	33	dep	PBA	861:863	arg1	endowed					872:878	endowed	872:878	endowed	872:878	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	0	34	theme	composite	61:69	arg1	hydrogels					71:79	composite hydrogels	61:79	composite hydrogels	61:79	Stretchable, self-healing and adhesive sodium alginate-based composite hydrogels as wearable strain sensors for expansion-contraction motion monitoring.					
35128552	0	34	theme	composite	61:69	arg1	sensors					100:106	wearable strain sensors	84:106	wearable strain sensors for expansion-contraction motion monitoring	84:150	Stretchable, self-healing and adhesive sodium alginate-based composite hydrogels as wearable strain sensors for expansion-contraction motion monitoring.					
35128552	3	35	theme	multifunctional	785:799	arg1	hydrogel					809:816	a multifunctional Alg-CNT hydrogel	783:816	a multifunctional Alg-CNT hydrogel	783:816	In this study, along with carbon nanotubes (CNTs) as conductive components, sodium alginate (Alg) modified with 3-aminophenylboronic acid (PBA) and dopamine (DA) were synthesized and employed as precursors to prepare a multifunctional Alg-CNT hydrogel.					
35128552	4	36	theme	rapid	900:904	arg1	property					919:926	a rapid self-healing property	898:926	a rapid self-healing property (30 s)	898:933	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	4	36	theme	rapid	900:904	arg1	s					932:932	30 s	929:932	30 s	929:932	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	4	37	theme	CNTs	961:964	arg1	introduction					945:956	the introduction	941:956	the introduction of CNTs	941:964	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	6	38	theme	mouse	1424:1428	arg1	heart					1430:1434	mouse heart beating	1424:1442	mouse heart beating	1424:1442	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	2	39	theme	parts	491:495	arg1	bending					451:457	bending	451:457	bending	451:457	However, the present motion detectors mainly focus on stretching, bending, and twisting of different body parts while the expansion-contraction motion has been rarely investigated.					
35128552	2	39	theme	parts	491:495	arg1	twisting					464:471	twisting	464:471	twisting	464:471	However, the present motion detectors mainly focus on stretching, bending, and twisting of different body parts while the expansion-contraction motion has been rarely investigated.					
35128552	2	39	theme	parts	491:495	arg1	stretching					439:448	stretching	439:448	stretching	439:448	However, the present motion detectors mainly focus on stretching, bending, and twisting of different body parts while the expansion-contraction motion has been rarely investigated.					
35128552	7	40	theme	potential	1614:1622	arg1	applications					1624:1635	its potential applications	1610:1635	its potential applications in personal health monitoring	1610:1665	Therefore, this multifunctional hydrogel is highly suitable for monitoring expansion-contraction motions, indicating its potential applications in personal health monitoring.					
35128552	6	41	dep	breathing	1410:1418	arg1	e.g.					1398:1401	e.g.	1398:1401	e.g.	1398:1401	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	4	42	theme	covalent	838:845	arg1	bonds					847:851	dynamic covalent bonds	830:851	dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s)	830:933	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	6	43	theme	strain	1197:1202	arg1	sensor					1204:1209	a strain sensor	1195:1209	a strain sensor	1195:1209	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	1	44	theme	Developing	153:162	arg1	hydrogels					180:188	Developing multifunctional hydrogels	153:188	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring	153:330	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired.					
35128552	6	45	theme	electrical	1473:1482	arg1	signals					1484:1490	periodic and repeatable electrical signals	1449:1490	periodic and repeatable electrical signals	1449:1490	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	1	46	theme	flexible	269:276	arg1	sensors					285:291	flexible strain sensors	269:291	flexible strain sensors for human motion and health monitoring	269:330	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired.					
35128552	4	47	theme	self-healing	906:917	arg1	property					919:926	a rapid self-healing property	898:926	a rapid self-healing property (30 s)	898:933	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	4	47	theme	self-healing	906:917	arg1	s					932:932	30 s	929:932	30 s	929:932	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	3	48	theme	3-aminophenylboronic	678:697	arg1	acid					699:702	3-aminophenylboronic acid	678:702	3-aminophenylboronic acid (PBA)	678:708	In this study, along with carbon nanotubes (CNTs) as conductive components, sodium alginate (Alg) modified with 3-aminophenylboronic acid (PBA) and dopamine (DA) were synthesized and employed as precursors to prepare a multifunctional Alg-CNT hydrogel.					
35128552	3	48	theme	3-aminophenylboronic	678:697	arg1	PBA					705:707	PBA	705:707	PBA	705:707	In this study, along with carbon nanotubes (CNTs) as conductive components, sodium alginate (Alg) modified with 3-aminophenylboronic acid (PBA) and dopamine (DA) were synthesized and employed as precursors to prepare a multifunctional Alg-CNT hydrogel.					
35128552	7	49	theme	personal	1640:1647	arg1	monitoring					1656:1665	personal health monitoring	1640:1665	personal health monitoring	1640:1665	Therefore, this multifunctional hydrogel is highly suitable for monitoring expansion-contraction motions, indicating its potential applications in personal health monitoring.					
35128552	1	50	theme	multifunctional	164:178	arg1	hydrogels					180:188	Developing multifunctional hydrogels	153:188	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring	153:330	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired.					
35128552	6	51	theme	repeatable	1462:1471	arg1	signals					1484:1490	periodic and repeatable electrical signals	1449:1490	periodic and repeatable electrical signals	1449:1490	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	1	52	theme	strain	278:283	arg1	sensors					285:291	flexible strain sensors	269:291	flexible strain sensors for human motion and health monitoring	269:330	Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired.					
35128552	4	53	with	endowed	872:878	arg1	property					919:926	a rapid self-healing property	898:926	a rapid self-healing property (30 s)	898:933	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	4	53	with	endowed	872:878	arg1	s					932:932	30 s	929:932	30 s	929:932	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	3	54	theme	Alg-CNT	801:807	arg1	hydrogel					809:816	a multifunctional Alg-CNT hydrogel	783:816	a multifunctional Alg-CNT hydrogel	783:816	In this study, along with carbon nanotubes (CNTs) as conductive components, sodium alginate (Alg) modified with 3-aminophenylboronic acid (PBA) and dopamine (DA) were synthesized and employed as precursors to prepare a multifunctional Alg-CNT hydrogel.					
35128552	4	55	theme	electrical	1014:1023	arg1	conductivity					1025:1036	electrical conductivity	1014:1036	electrical conductivity	1014:1036	The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel.					
35128552	3	56	theme	sodium	642:647	arg1	Alg					659:661	Alg	659:661	Alg	659:661	In this study, along with carbon nanotubes (CNTs) as conductive components, sodium alginate (Alg) modified with 3-aminophenylboronic acid (PBA) and dopamine (DA) were synthesized and employed as precursors to prepare a multifunctional Alg-CNT hydrogel.					
35128552	3	56	theme	sodium	642:647	arg1	precursors					761:770	precursors	761:770	precursors	761:770	In this study, along with carbon nanotubes (CNTs) as conductive components, sodium alginate (Alg) modified with 3-aminophenylboronic acid (PBA) and dopamine (DA) were synthesized and employed as precursors to prepare a multifunctional Alg-CNT hydrogel.					
35128552	3	56	theme	sodium	642:647	arg1	alginate					649:656	sodium alginate	642:656	sodium alginate (Alg) modified with 3-aminophenylboronic acid (PBA) and dopamine (DA)	642:726	In this study, along with carbon nanotubes (CNTs) as conductive components, sodium alginate (Alg) modified with 3-aminophenylboronic acid (PBA) and dopamine (DA) were synthesized and employed as precursors to prepare a multifunctional Alg-CNT hydrogel.					
35128552	2	57	theme	motion	406:411	arg1	detectors					413:421	the present motion detectors	394:421	the present motion detectors	394:421	However, the present motion detectors mainly focus on stretching, bending, and twisting of different body parts while the expansion-contraction motion has been rarely investigated.					
35128552	6	58	theme	periodic	1449:1456	arg1	signals					1484:1490	periodic and repeatable electrical signals	1449:1490	periodic and repeatable electrical signals	1449:1490	When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals.					
35128552	5	59	theme	various	1162:1168	arg1	substrates					1170:1179	various substrates	1162:1179	various substrates	1162:1179	Moreover, the as-prepared hydrogel displayed a satisfactory stretchability (500%) and self-adhesiveness to various substrates.					
35904200	12	0	theme	PRACTICAL	1560:1568	arg1	APPLICATION					1570:1580	PRACTICAL APPLICATION	1560:1580	PRACTICAL APPLICATION: Substituting wheat flour with ayocote bean flour	1560:1630	PRACTICAL APPLICATION: Substituting wheat flour with ayocote bean flour improved the nutritional value of bread.					
35904200	6	1	theme	control	897:903	arg1	bread					905:909	the control bread	893:909	the control bread	893:909	The sensory attribute scores of 10% AGF bread were comparable to those of the control bread.					
35904200	16	2	theme	total	1962:1966	arg1	starch					1979:1984	low total digestible starch	1958:1984	low total digestible starch	1958:1984	Composite bread had high resistant starch and low total digestible starch.					
35904200	3	3	theme	crude	537:541	arg1	content					549:555	13%, 51%, and 132% higher protein, mineral, and crude fiber content	489:555	content	549:555	Supplemented breads had 13%, 51%, and 132% higher protein, mineral, and crude fiber content, respectively, than control bread (100% wheat).					
35904200	7	4	theme	in	940:941	arg1	digestibility					957:969	the in vitro protein digestibility	936:969	the in vitro protein digestibility	936:969	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	13	5	theme	composite	1737:1745	arg1	dough					1747:1751	composite dough	1737:1751	composite dough	1737:1751	Germination of ayocote beans decreased the cooking stability of composite dough.					
35904200	7	6	from	AGF	1031:1033	arg1	effect					985:990	the effect	981:990	the effect	981:990	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	7	6	from	AGF	1031:1033	arg1	pronounced					1001:1010	pronounced	1001:1010	pronounced	1001:1010	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	7	7	theme	%	1029:1029	arg1	AGF					1031:1033	20% AGF	1027:1033	20% AGF	1027:1033	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	1	8	theme	%	212:212	arg1	partial					281:287	partial	281:287	partial	281:287	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	1	8	theme	%	212:212	arg1	potential					192:200	the potential	188:200	the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour	188:274	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	5	9	theme	Sensory	698:704	arg1	analysis					706:713	Sensory analysis	698:713	Sensory analysis	698:713	Sensory analysis revealed that germination could improve the taste and smell of breads produced with ayocote bean flour.					
35904200	2	10	theme	dough	448:452	arg1	stability					454:462	the dough stability	444:462	the dough stability	444:462	Substitution with both ayocote bean flours modified the water absorption and development time while maintaining the dough stability.					
35904200	8	11	theme	limiting	1047:1054	arg1	score					1067:1071	The limiting amino acid score	1043:1071	The limiting amino acid score of supplemented breads	1043:1094	The limiting amino acid score of supplemented breads increased up to 70%, which improved their protein digestibility-corrected amino acid scores.					
35904200	7	12	dep	in	940:941	arg1	vitro					943:947	vitro	943:947	vitro	943:947	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	1	13	theme	flour	270:274	arg1	ARF					231:233	raw (ARF) and germinated (AGF) ayocote bean flour	226:274	ARF	231:233	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	1	13	theme	flour	270:274	arg1	%					221:221	30%	219:221	30%	219:221	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	1	13	theme	flour	270:274	arg1	flour					270:274	raw (ARF) and germinated (AGF) ayocote bean flour	226:274	flour	270:274	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	1	13	theme	flour	270:274	arg1	%					207:207	10%	205:207	10%	205:207	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	1	13	theme	flour	270:274	arg1	%					212:212	20%	210:212	20%	210:212	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	7	14	from	pronounced	1001:1010	arg1	ARF					1019:1021	10% ARF	1015:1021	10% ARF	1015:1021	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	7	14	from	pronounced	1001:1010	arg1	AGF					1031:1033	20% AGF	1027:1033	20% AGF	1027:1033	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	8	15	theme	acid	1062:1065	arg1	score					1067:1071	The limiting amino acid score	1043:1071	The limiting amino acid score of supplemented breads	1043:1094	The limiting amino acid score of supplemented breads increased up to 70%, which improved their protein digestibility-corrected amino acid scores.					
35904200	16	16	theme	resistant	1937:1945	arg1	starch					1947:1952	high resistant starch	1932:1952	high resistant starch	1932:1952	Composite bread had high resistant starch and low total digestible starch.					
35904200	0	17	theme	nutritional	114:124	arg1	properties					126:135	the nutritional properties	110:135	the nutritional properties	110:135	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	2	18	with	Substitution	332:343	arg1	flours					368:373	both ayocote bean flours	350:373	both ayocote bean flours	350:373	Substitution with both ayocote bean flours modified the water absorption and development time while maintaining the dough stability.					
35904200	9	19	theme	starch	1261:1266	arg1	values					1268:1273	resistant starch values	1251:1273	resistant starch values	1251:1273	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	7	20	from	ARF	1019:1021	arg1	effect					985:990	the effect	981:990	the effect	981:990	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	7	20	from	ARF	1019:1021	arg1	pronounced					1001:1010	pronounced	1001:1010	pronounced	1001:1010	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	8	21	theme	breads	1089:1094	arg1	score					1067:1071	The limiting amino acid score	1043:1071	The limiting amino acid score of supplemented breads	1043:1094	The limiting amino acid score of supplemented breads increased up to 70%, which improved their protein digestibility-corrected amino acid scores.					
35904200	13	22	theme	ayocote	1688:1694	arg1	beans					1696:1700	ayocote beans	1688:1700	ayocote beans	1688:1700	Germination of ayocote beans decreased the cooking stability of composite dough.					
35904200	11	23	theme	considerable	1502:1513	arg1	decrease					1515:1522	a considerable decrease	1500:1522	a considerable decrease in physical and sensory properties	1500:1557	However, low substitution levels should be selected to avoid a considerable decrease in physical and sensory properties.					
35904200	1	24	theme	wheat	304:308	arg1	flour					310:314	wheat flour	304:314	wheat flour in breadmaking	304:329	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	2	25	theme	water	388:392	arg1	absorption					394:403	water absorption	388:403	water absorption	388:403	Substitution with both ayocote bean flours modified the water absorption and development time while maintaining the dough stability.					
35904200	7	26	theme	20	1027:1028	arg1	%					1029:1029	%	1029:1029	%	1029:1029	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	6	27	theme	attribute	831:839	arg1	scores					841:846	The sensory attribute scores	819:846	The sensory attribute scores of 10% AGF bread	819:863	The sensory attribute scores of 10% AGF bread were comparable to those of the control bread.					
35904200	6	27	theme	attribute	831:839	arg1	comparable					870:879	comparable	870:879	comparable	870:879	The sensory attribute scores of 10% AGF bread were comparable to those of the control bread.					
35904200	0	28	theme	germinated	55:64	arg1	flour					101:105	germinated ayocote bean (Phaseolus coccineus) flour	55:105	germinated ayocote bean (Phaseolus coccineus) flour	55:105	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	1	29	theme	germinated	240:249	arg1	flour					270:274	raw (ARF) and germinated (AGF) ayocote bean flour	226:274	flour	270:274	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	9	30	theme	digestible	1299:1308	arg1	starch					1310:1315	the total digestible starch	1289:1315	the total digestible starch of breads	1289:1325	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	11	31	theme	substitution	1452:1463	arg1	levels					1465:1470	low substitution levels	1448:1470	low substitution levels	1448:1470	However, low substitution levels should be selected to avoid a considerable decrease in physical and sensory properties.					
35904200	0	32	from	Effects	0:6	arg1	properties					126:135	the nutritional properties	110:135	the nutritional properties	110:135	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	0	32	from	Effects	0:6	arg1	quality					141:147	quality	141:147	quality of bread	141:156	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	0	33	theme	bean	74:77	arg1	flour					101:105	germinated ayocote bean (Phaseolus coccineus) flour	55:105	germinated ayocote bean (Phaseolus coccineus) flour	55:105	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	1	34	theme	AGF	252:254	arg1	flour					270:274	raw (ARF) and germinated (AGF) ayocote bean flour	226:274	flour	270:274	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	13	35	theme	beans	1696:1700	arg1	Germination					1673:1683	Germination	1673:1683	Germination of ayocote beans	1673:1700	Germination of ayocote beans decreased the cooking stability of composite dough.					
35904200	0	36	theme	Phaseolus	80:88	arg1	flour					101:105	germinated ayocote bean (Phaseolus coccineus) flour	55:105	germinated ayocote bean (Phaseolus coccineus) flour	55:105	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	1	37	theme	ayocote	257:263	arg1	flour					270:274	raw (ARF) and germinated (AGF) ayocote bean flour	226:274	flour	270:274	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	5	38	theme	bean	807:810	arg1	flour					812:816	ayocote bean flour	799:816	ayocote bean flour	799:816	Sensory analysis revealed that germination could improve the taste and smell of breads produced with ayocote bean flour.					
35904200	8	39	theme	protein	1138:1144	arg1	acid					1176:1179	their protein digestibility-corrected amino acid	1132:1179	their protein digestibility-corrected amino acid scores	1132:1186	The limiting amino acid score of supplemented breads increased up to 70%, which improved their protein digestibility-corrected amino acid scores.					
35904200	14	40	theme	ayocote	1775:1781	arg1	flour					1783:1787	ayocote flour	1775:1787	ayocote flour	1775:1787	Bread fortified with ayocote flour had high levels of essential amino acids.					
35904200	8	41	theme	amino	1170:1174	arg1	acid					1176:1179	their protein digestibility-corrected amino acid	1132:1179	their protein digestibility-corrected amino acid scores	1132:1186	The limiting amino acid score of supplemented breads increased up to 70%, which improved their protein digestibility-corrected amino acid scores.					
35904200	15	42	theme	amino	1893:1897	arg1	scores					1904:1909	high limiting amino acid scores	1879:1909	high limiting amino acid scores	1879:1909	Bread with raw or germinated ayocote flours had high limiting amino acid scores.					
35904200	5	43	theme	breads	778:783	arg1	smell					769:773	smell	769:773	smell	769:773	Sensory analysis revealed that germination could improve the taste and smell of breads produced with ayocote bean flour.					
35904200	5	43	theme	breads	778:783	arg1	taste					759:763	taste	759:763	taste	759:763	Sensory analysis revealed that germination could improve the taste and smell of breads produced with ayocote bean flour.					
35904200	6	44	theme	bread	859:863	arg1	scores					841:846	The sensory attribute scores	819:846	The sensory attribute scores of 10% AGF bread	819:863	The sensory attribute scores of 10% AGF bread were comparable to those of the control bread.					
35904200	6	44	theme	bread	859:863	arg1	comparable					870:879	comparable	870:879	comparable	870:879	The sensory attribute scores of 10% AGF bread were comparable to those of the control bread.					
35904200	2	45	theme	bean	363:366	arg1	flours					368:373	both ayocote bean flours	350:373	both ayocote bean flours	350:373	Substitution with both ayocote bean flours modified the water absorption and development time while maintaining the dough stability.					
35904200	4	46	theme	crumb	641:645	arg1	firmness					647:654	crumb firmness	641:654	crumb firmness	641:654	The breadmaking features, color and crumb firmness, were affected by the substitution level.					
35904200	4	46	theme	crumb	641:645	arg1	features					621:628	The breadmaking features	605:628	The breadmaking features	605:628	The breadmaking features, color and crumb firmness, were affected by the substitution level.					
35904200	10	47	theme	bread	1432:1436	arg1	properties					1418:1427	the nutritional properties	1402:1427	the nutritional properties of bread	1402:1436	Thus, the results showed that substituting wheat with ARF or AGF improves the nutritional properties of bread.					
35904200	12	48	theme	nutritional	1645:1655	arg1	value					1657:1661	the nutritional value	1641:1661	the nutritional value of bread	1641:1670	PRACTICAL APPLICATION: Substituting wheat flour with ayocote bean flour improved the nutritional value of bread.					
35904200	15	49	theme	acid	1899:1902	arg1	scores					1904:1909	high limiting amino acid scores	1879:1909	high limiting amino acid scores	1879:1909	Bread with raw or germinated ayocote flours had high limiting amino acid scores.					
35904200	6	50	theme	%	853:853	arg1	bread					859:863	10% AGF bread	851:863	10% AGF bread	851:863	The sensory attribute scores of 10% AGF bread were comparable to those of the control bread.					
35904200	1	51	from	flour	310:314	arg1	breadmaking					319:329	breadmaking	319:329	breadmaking	319:329	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	0	52	theme	flour	37:41	arg1	substitution					15:26	the substitution	11:26	the substitution of wheat flour with raw	11:50	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	0	52	theme	flour	37:41	arg1	flour					101:105	germinated ayocote bean (Phaseolus coccineus) flour	55:105	germinated ayocote bean (Phaseolus coccineus) flour	55:105	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	15	53	theme	limiting	1884:1891	arg1	scores					1904:1909	high limiting amino acid scores	1879:1909	high limiting amino acid scores	1879:1909	Bread with raw or germinated ayocote flours had high limiting amino acid scores.					
35904200	14	54	theme	amino	1818:1822	arg1	acids					1824:1828	essential amino acids	1808:1828	essential amino acids	1808:1828	Bread fortified with ayocote flour had high levels of essential amino acids.					
35904200	12	55	theme	bean	1621:1624	arg1	flour					1626:1630	ayocote bean flour	1613:1630	ayocote bean flour	1613:1630	PRACTICAL APPLICATION: Substituting wheat flour with ayocote bean flour improved the nutritional value of bread.					
35904200	1	56	dep	partial	281:287	arg1	substitute					289:298	substitute	289:298	substitute for wheat flour in breadmaking	289:329	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	16	57	theme	low	1958:1960	arg1	starch					1979:1984	low total digestible starch	1958:1984	low total digestible starch	1958:1984	Composite bread had high resistant starch and low total digestible starch.					
35904200	3	58	theme	mineral	524:530	arg1	protein					515:521	13%, 51%, and 132% higher protein, mineral, and crude fiber content	489:555	protein	515:521	Supplemented breads had 13%, 51%, and 132% higher protein, mineral, and crude fiber content, respectively, than control bread (100% wheat).					
35904200	9	59	with	Supplementation	1189:1203	arg1	AGF					1237:1239	AGF	1237:1239	AGF	1237:1239	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	9	59	with	Supplementation	1189:1203	arg1	%					1212:1212	20%	1210:1212	20%	1210:1212	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	9	59	with	Supplementation	1189:1203	arg1	%					1219:1219	30%	1217:1219	30%	1217:1219	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	9	59	with	Supplementation	1189:1203	arg1	ARF					1229:1231	ARF	1229:1231	ARF	1229:1231	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	15	60	theme	ayocote	1860:1866	arg1	flours					1868:1873	raw or germinated ayocote flours	1842:1873	flours	1868:1873	Bread with raw or germinated ayocote flours had high limiting amino acid scores.					
35904200	7	61	dep	ARF	1019:1021	arg1	breads					1035:1040	breads	1035:1040	breads	1035:1040	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	16	62	theme	digestible	1968:1977	arg1	starch					1979:1984	low total digestible starch	1958:1984	low total digestible starch	1958:1984	Composite bread had high resistant starch and low total digestible starch.					
35904200	7	63	theme	%	1017:1017	arg1	ARF					1019:1021	10% ARF	1015:1021	10% ARF	1015:1021	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	0	64	theme	bread	152:156	arg1	properties					126:135	the nutritional properties	110:135	the nutritional properties	110:135	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	0	64	theme	bread	152:156	arg1	quality					141:147	quality	141:147	quality of bread	141:156	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	1	65	theme	%	207:207	arg1	partial					281:287	partial	281:287	partial	281:287	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	1	65	theme	%	207:207	arg1	potential					192:200	the potential	188:200	the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour	188:274	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	4	66	theme	substitution	678:689	arg1	level					691:695	the substitution level	674:695	the substitution level	674:695	The breadmaking features, color and crumb firmness, were affected by the substitution level.					
35904200	11	67	theme	low	1448:1450	arg1	levels					1465:1470	low substitution levels	1448:1470	low substitution levels	1448:1470	However, low substitution levels should be selected to avoid a considerable decrease in physical and sensory properties.					
35904200	3	68	contain	had	485:487	arg2	content					549:555	13%, 51%, and 132% higher protein, mineral, and crude fiber content	489:555	content	549:555	Supplemented breads had 13%, 51%, and 132% higher protein, mineral, and crude fiber content, respectively, than control bread (100% wheat).					
35904200	3	68	contain	had	485:487	arg2	protein					515:521	13%, 51%, and 132% higher protein, mineral, and crude fiber content	489:555	protein	515:521	Supplemented breads had 13%, 51%, and 132% higher protein, mineral, and crude fiber content, respectively, than control bread (100% wheat).					
35904200	3	68	contain	had	485:487	arg1	breads					478:483	Supplemented breads	465:483	Supplemented breads	465:483	Supplemented breads had 13%, 51%, and 132% higher protein, mineral, and crude fiber content, respectively, than control bread (100% wheat).					
35904200	3	69	theme	fiber	543:547	arg1	content					549:555	13%, 51%, and 132% higher protein, mineral, and crude fiber content	489:555	content	549:555	Supplemented breads had 13%, 51%, and 132% higher protein, mineral, and crude fiber content, respectively, than control bread (100% wheat).					
35904200	13	70	theme	dough	1747:1751	arg1	stability					1724:1732	the cooking stability	1712:1732	the cooking stability of composite dough	1712:1751	Germination of ayocote beans decreased the cooking stability of composite dough.					
35904200	16	71	contain	had	1928:1930	arg2	starch					1947:1952	high resistant starch	1932:1952	high resistant starch	1932:1952	Composite bread had high resistant starch and low total digestible starch.					
35904200	16	71	contain	had	1928:1930	arg1	bread					1922:1926	Composite bread	1912:1926	Composite bread	1912:1926	Composite bread had high resistant starch and low total digestible starch.					
35904200	16	71	contain	had	1928:1930	arg2	starch					1979:1984	low total digestible starch	1958:1984	low total digestible starch	1958:1984	Composite bread had high resistant starch and low total digestible starch.					
35904200	3	72	theme	control	577:583	arg1	bread					585:589	control bread	577:589	control bread (100% wheat)	577:602	Supplemented breads had 13%, 51%, and 132% higher protein, mineral, and crude fiber content, respectively, than control bread (100% wheat).					
35904200	3	72	theme	control	577:583	arg1	wheat					597:601	100% wheat	592:601	100% wheat	592:601	Supplemented breads had 13%, 51%, and 132% higher protein, mineral, and crude fiber content, respectively, than control bread (100% wheat).					
35904200	7	73	theme	protein	949:955	arg1	digestibility					957:969	the in vitro protein digestibility	936:969	the in vitro protein digestibility	936:969	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	13	74	theme	cooking	1716:1722	arg1	stability					1724:1732	the cooking stability	1712:1732	the cooking stability of composite dough	1712:1751	Germination of ayocote beans decreased the cooking stability of composite dough.					
35904200	3	75	theme	Supplemented	465:476	arg1	breads					478:483	Supplemented breads	465:483	Supplemented breads	465:483	Supplemented breads had 13%, 51%, and 132% higher protein, mineral, and crude fiber content, respectively, than control bread (100% wheat).					
35904200	3	76	theme	%	595:595	arg1	bread					585:589	control bread	577:589	control bread (100% wheat)	577:602	Supplemented breads had 13%, 51%, and 132% higher protein, mineral, and crude fiber content, respectively, than control bread (100% wheat).					
35904200	3	76	theme	%	595:595	arg1	wheat					597:601	100% wheat	592:601	100% wheat	592:601	Supplemented breads had 13%, 51%, and 132% higher protein, mineral, and crude fiber content, respectively, than control bread (100% wheat).					
35904200	0	77	theme	flour	101:105	arg1	Effects					0:6	Effects	0:6	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.	0:157	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	9	78	theme	ARF	1229:1231	arg1	AGF					1237:1239	AGF	1237:1239	AGF	1237:1239	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	9	78	theme	ARF	1229:1231	arg1	%					1212:1212	20%	1210:1212	20%	1210:1212	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	9	78	theme	ARF	1229:1231	arg1	%					1219:1219	30%	1217:1219	30%	1217:1219	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	9	78	theme	ARF	1229:1231	arg1	ARF					1229:1231	ARF	1229:1231	ARF	1229:1231	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	14	79	theme	high	1793:1796	arg1	levels					1798:1803	high levels	1793:1803	high levels of essential amino acids	1793:1828	Bread fortified with ayocote flour had high levels of essential amino acids.					
35904200	15	80	with	Bread	1831:1835	arg1	flours					1868:1873	raw or germinated ayocote flours	1842:1873	flours	1868:1873	Bread with raw or germinated ayocote flours had high limiting amino acid scores.					
35904200	9	81	theme	AGF	1237:1239	arg1	AGF					1237:1239	AGF	1237:1239	AGF	1237:1239	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	9	81	theme	AGF	1237:1239	arg1	%					1212:1212	20%	1210:1212	20%	1210:1212	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	9	81	theme	AGF	1237:1239	arg1	%					1219:1219	30%	1217:1219	30%	1217:1219	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	9	81	theme	AGF	1237:1239	arg1	ARF					1229:1231	ARF	1229:1231	ARF	1229:1231	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	8	82	theme	amino	1056:1060	arg1	score					1067:1071	The limiting amino acid score	1043:1071	The limiting amino acid score of supplemented breads	1043:1094	The limiting amino acid score of supplemented breads increased up to 70%, which improved their protein digestibility-corrected amino acid scores.					
35904200	9	83	theme	resistant	1251:1259	arg1	values					1268:1273	resistant starch values	1251:1273	resistant starch values	1251:1273	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	6	84	theme	10	851:852	arg1	%					853:853	%	853:853	%	853:853	The sensory attribute scores of 10% AGF bread were comparable to those of the control bread.					
35904200	11	85	from	decrease	1515:1522	arg1	properties					1548:1557	physical and sensory properties	1527:1557	physical and sensory properties	1527:1557	However, low substitution levels should be selected to avoid a considerable decrease in physical and sensory properties.					
35904200	8	86	theme	supplemented	1076:1087	arg1	breads					1089:1094	supplemented breads	1076:1094	supplemented breads	1076:1094	The limiting amino acid score of supplemented breads increased up to 70%, which improved their protein digestibility-corrected amino acid scores.					
35904200	0	87	with	flour	101:105	arg1	raw					48:50	raw	48:50	raw	48:50	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	15	88	contain	had	1875:1877	arg2	scores					1904:1909	high limiting amino acid scores	1879:1909	high limiting amino acid scores	1879:1909	Bread with raw or germinated ayocote flours had high limiting amino acid scores.					
35904200	15	88	contain	had	1875:1877	arg1	Bread					1831:1835	Bread	1831:1835	Bread with raw or germinated ayocote flours	1831:1873	Bread with raw or germinated ayocote flours had high limiting amino acid scores.					
35904200	16	89	theme	Composite	1912:1920	arg1	bread					1922:1926	Composite bread	1912:1926	Composite bread	1912:1926	Composite bread had high resistant starch and low total digestible starch.					
35904200	7	90	theme	10	1015:1016	arg1	%					1017:1017	%	1017:1017	%	1017:1017	Supplementation reduced the in vitro protein digestibility, although the effect was less pronounced in 10% ARF and 20% AGF breads.					
35904200	0	91	theme	ayocote	66:72	arg1	flour					101:105	germinated ayocote bean (Phaseolus coccineus) flour	55:105	germinated ayocote bean (Phaseolus coccineus) flour	55:105	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	9	92	theme	total	1293:1297	arg1	starch					1310:1315	the total digestible starch	1289:1315	the total digestible starch of breads	1289:1325	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	6	93	theme	sensory	823:829	arg1	scores					841:846	The sensory attribute scores	819:846	The sensory attribute scores of 10% AGF bread	819:863	The sensory attribute scores of 10% AGF bread were comparable to those of the control bread.					
35904200	6	93	theme	sensory	823:829	arg1	comparable					870:879	comparable	870:879	comparable	870:879	The sensory attribute scores of 10% AGF bread were comparable to those of the control bread.					
35904200	11	94	theme	sensory	1540:1546	arg1	properties					1548:1557	physical and sensory properties	1527:1557	physical and sensory properties	1527:1557	However, low substitution levels should be selected to avoid a considerable decrease in physical and sensory properties.					
35904200	15	95	theme	raw	1842:1844	arg1	flours					1868:1873	raw or germinated ayocote flours	1842:1873	flours	1868:1873	Bread with raw or germinated ayocote flours had high limiting amino acid scores.					
35904200	2	96	theme	development	409:419	arg1	time					421:424	the water absorption and development time	384:424	the water absorption and development time	384:424	Substitution with both ayocote bean flours modified the water absorption and development time while maintaining the dough stability.					
35904200	5	97	dep	taste	759:763	arg1	the					755:757	the	755:757	the	755:757	Sensory analysis revealed that germination could improve the taste and smell of breads produced with ayocote bean flour.					
35904200	12	98	dep	APPLICATION	1570:1580	arg1	Substituting					1583:1594	Substituting	1583:1594	Substituting wheat flour with ayocote bean flour	1583:1630	PRACTICAL APPLICATION: Substituting wheat flour with ayocote bean flour improved the nutritional value of bread.					
35904200	11	99	theme	physical	1527:1534	arg1	properties					1548:1557	physical and sensory properties	1527:1557	physical and sensory properties	1527:1557	However, low substitution levels should be selected to avoid a considerable decrease in physical and sensory properties.					
35904200	0	100	theme	coccineus	90:98	arg1	flour					101:105	germinated ayocote bean (Phaseolus coccineus) flour	55:105	germinated ayocote bean (Phaseolus coccineus) flour	55:105	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	12	101	theme	bread	1666:1670	arg1	value					1657:1661	the nutritional value	1641:1661	the nutritional value of bread	1641:1670	PRACTICAL APPLICATION: Substituting wheat flour with ayocote bean flour improved the nutritional value of bread.					
35904200	9	102	theme	breads	1320:1325	arg1	starch					1310:1315	the total digestible starch	1289:1315	the total digestible starch of breads	1289:1325	Supplementation with 20% or 30% of both ARF and AGF increased resistant starch values and decreased the total digestible starch of breads.					
35904200	2	103	theme	absorption	394:403	arg1	time					421:424	the water absorption and development time	384:424	the water absorption and development time	384:424	Substitution with both ayocote bean flours modified the water absorption and development time while maintaining the dough stability.					
35904200	16	104	theme	high	1932:1935	arg1	starch					1947:1952	high resistant starch	1932:1952	high resistant starch	1932:1952	Composite bread had high resistant starch and low total digestible starch.					
35904200	10	105	theme	nutritional	1406:1416	arg1	properties					1418:1427	the nutritional properties	1402:1427	the nutritional properties of bread	1402:1436	Thus, the results showed that substituting wheat with ARF or AGF improves the nutritional properties of bread.					
35904200	1	106	theme	bean	265:268	arg1	flour					270:274	raw (ARF) and germinated (AGF) ayocote bean flour	226:274	flour	270:274	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	0	107	theme	substitution	15:26	arg1	Effects					0:6	Effects	0:6	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.	0:157	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	5	108	theme	ayocote	799:805	arg1	flour					812:816	ayocote bean flour	799:816	ayocote bean flour	799:816	Sensory analysis revealed that germination could improve the taste and smell of breads produced with ayocote bean flour.					
35904200	3	109	theme	higher	508:513	arg1	protein					515:521	13%, 51%, and 132% higher protein, mineral, and crude fiber content	489:555	protein	515:521	Supplemented breads had 13%, 51%, and 132% higher protein, mineral, and crude fiber content, respectively, than control bread (100% wheat).					
35904200	8	110	theme	digestibility-corrected	1146:1168	arg1	acid					1176:1179	their protein digestibility-corrected amino acid	1132:1179	their protein digestibility-corrected amino acid scores	1132:1186	The limiting amino acid score of supplemented breads increased up to 70%, which improved their protein digestibility-corrected amino acid scores.					
35904200	0	111	theme	wheat	31:35	arg1	flour					37:41	wheat flour	31:41	wheat flour	31:41	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	1	112	theme	%	221:221	arg1	partial					281:287	partial	281:287	partial	281:287	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	1	112	theme	%	221:221	arg1	potential					192:200	the potential	188:200	the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour	188:274	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	8	113	dep	70	1112:1113	arg1	to					1109:1110	to	1109:1110	to	1109:1110	The limiting amino acid score of supplemented breads increased up to 70%, which improved their protein digestibility-corrected amino acid scores.					
35904200	6	114	theme	AGF	855:857	arg1	bread					859:863	10% AGF bread	851:863	10% AGF bread	851:863	The sensory attribute scores of 10% AGF bread were comparable to those of the control bread.					
35904200	2	115	theme	ayocote	355:361	arg1	flours					368:373	both ayocote bean flours	350:373	both ayocote bean flours	350:373	Substitution with both ayocote bean flours modified the water absorption and development time while maintaining the dough stability.					
35904200	8	116	theme	acid	1176:1179	arg1	scores					1181:1186	their protein digestibility-corrected amino acid scores	1132:1186	their protein digestibility-corrected amino acid scores	1132:1186	The limiting amino acid score of supplemented breads increased up to 70%, which improved their protein digestibility-corrected amino acid scores.					
35904200	0	117	with	substitution	15:26	arg1	raw					48:50	raw	48:50	raw	48:50	Effects of the substitution of wheat flour with raw or germinated ayocote bean (Phaseolus coccineus) flour on the nutritional properties and quality of bread.					
35904200	1	118	theme	raw	226:228	arg1	ARF					231:233	raw (ARF) and germinated (AGF) ayocote bean flour	226:274	ARF	231:233	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	12	119	theme	ayocote	1613:1619	arg1	flour					1626:1630	ayocote bean flour	1613:1630	ayocote bean flour	1613:1630	PRACTICAL APPLICATION: Substituting wheat flour with ayocote bean flour improved the nutritional value of bread.					
35904200	15	120	theme	high	1879:1882	arg1	scores					1904:1909	high limiting amino acid scores	1879:1909	high limiting amino acid scores	1879:1909	Bread with raw or germinated ayocote flours had high limiting amino acid scores.					
35904200	14	121	theme	acids	1824:1828	arg1	levels					1798:1803	high levels	1793:1803	high levels of essential amino acids	1793:1828	Bread fortified with ayocote flour had high levels of essential amino acids.					
35904200	1	122	theme	ARF	231:233	arg1	ARF					231:233	raw (ARF) and germinated (AGF) ayocote bean flour	226:274	ARF	231:233	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	1	122	theme	ARF	231:233	arg1	%					221:221	30%	219:221	30%	219:221	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	1	122	theme	ARF	231:233	arg1	flour					270:274	raw (ARF) and germinated (AGF) ayocote bean flour	226:274	flour	270:274	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	1	122	theme	ARF	231:233	arg1	%					207:207	10%	205:207	10%	205:207	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	1	122	theme	ARF	231:233	arg1	%					212:212	20%	210:212	20%	210:212	This study aimed to evaluate the potential of 10%, 20%, and 30% of raw (ARF) and germinated (AGF) ayocote bean flour as a partial substitute for wheat flour in breadmaking.					
35904200	12	123	theme	wheat	1596:1600	arg1	flour					1602:1606	wheat flour	1596:1606	wheat flour	1596:1606	PRACTICAL APPLICATION: Substituting wheat flour with ayocote bean flour improved the nutritional value of bread.					
35904200	14	124	theme	essential	1808:1816	arg1	acids					1824:1828	essential amino acids	1808:1828	essential amino acids	1808:1828	Bread fortified with ayocote flour had high levels of essential amino acids.					
35904200	4	125	theme	breadmaking	609:619	arg1	color					631:635	color	631:635	color	631:635	The breadmaking features, color and crumb firmness, were affected by the substitution level.					
35904200	4	125	theme	breadmaking	609:619	arg1	firmness					647:654	crumb firmness	641:654	crumb firmness	641:654	The breadmaking features, color and crumb firmness, were affected by the substitution level.					
35904200	4	125	theme	breadmaking	609:619	arg1	features					621:628	The breadmaking features	605:628	The breadmaking features	605:628	The breadmaking features, color and crumb firmness, were affected by the substitution level.					
35904200	15	126	theme	germinated	1849:1858	arg1	flours					1868:1873	raw or germinated ayocote flours	1842:1873	flours	1868:1873	Bread with raw or germinated ayocote flours had high limiting amino acid scores.					
35904200	14	127	contain	had	1789:1791	arg2	levels					1798:1803	high levels	1793:1803	high levels of essential amino acids	1793:1828	Bread fortified with ayocote flour had high levels of essential amino acids.					
35904200	14	127	contain	had	1789:1791	arg1	Bread					1754:1758	Bread	1754:1758	Bread fortified with ayocote flour	1754:1787	Bread fortified with ayocote flour had high levels of essential amino acids.					
37047002	8	0	theme	waste	1128:1132	arg1	nanofibers					1109:1118	the nanofibers	1105:1118	the nanofibers of palm waste, cotton fibers, and filter paper	1105:1165	The surface charge densities were -94.0, -80.7, and -90.6 mV for the nanofibers of palm waste, cotton fibers, and filter paper, respectively.					
37047002	6	1	theme	advanced	900:907	arg1	techniques					920:929	various advanced analytical techniques	892:929	various advanced analytical techniques	892:929	The physical, chemical, and structural properties of nanofibers were studied using various advanced analytical techniques.					
37047002	11	2	theme	groups	1581:1586	arg1	ζ-potential					1604:1614	the surface ζ-potential	1592:1614	the surface ζ-potential of the membrane frameworks' interaction	1592:1654	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	11	2	theme	groups	1581:1586	arg1	behavior					1548:1555	the behavior	1544:1555	the behavior of available functional groups	1544:1586	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	11	3	theme	blue	1491:1494	arg1	solutions					1520:1528	model solutions	1514:1528	model solutions	1514:1528	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	11	3	theme	blue	1491:1494	arg1	dyes					1496:1499	Congo red and methylene blue dyes	1467:1499	Congo red and methylene blue dyes	1467:1499	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	8	4	theme	cotton	1135:1140	arg1	fibers					1142:1147	cotton fibers	1135:1147	cotton fibers	1135:1147	The surface charge densities were -94.0, -80.7, and -90.6 mV for the nanofibers of palm waste, cotton fibers, and filter paper, respectively.					
37047002	12	5	theme	highest	1699:1705	arg1	efficiency					1719:1728	the highest dye removal efficiency	1695:1728	the highest dye removal efficiency	1695:1728	The membrane made from palm waste had the highest dye removal efficiency, and it was 23% for Congo red and 44% for methylene blue.					
37047002	10	6	theme	palm-waste	1355:1364	arg1	membrane					1366:1373	the palm-waste membrane	1351:1373	the palm-waste membrane	1351:1373	The results showed that the average pore size of the palm-waste membrane was 1.185 nm, while it was 1.875 nm for membrane from waste cotton fibers and filter paper.					
37047002	3	7	theme	treatment	538:546	arg1	purposes					548:555	wastewater treatment purposes	527:555	wastewater treatment purposes	527:555	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	12	8	theme	removal	1711:1717	arg1	efficiency					1719:1728	the highest dye removal efficiency	1695:1728	the highest dye removal efficiency	1695:1728	The membrane made from palm waste had the highest dye removal efficiency, and it was 23% for Congo red and 44% for methylene blue.					
37047002	8	9	theme	filter	1154:1159	arg1	paper					1161:1165	filter paper	1154:1165	filter paper	1154:1165	The surface charge densities were -94.0, -80.7, and -90.6 mV for the nanofibers of palm waste, cotton fibers, and filter paper, respectively.					
37047002	12	10	theme	palm	1680:1683	arg1	waste					1685:1689	palm waste	1680:1689	palm waste	1680:1689	The membrane made from palm waste had the highest dye removal efficiency, and it was 23% for Congo red and 44% for methylene blue.					
37047002	13	11	theme	postconsumer	1864:1875	arg1	waste					1902:1906	postconsumer textile and agricultural waste	1864:1906	postconsumer textile and agricultural waste	1864:1906	This study provides insights into the challenges associated with the use of postconsumer textile and agricultural waste, which can be potentially used in high-performance liquid filtration devices for a more sustainable society.					
37047002	4	12	theme	cotton	628:633	arg1	fabrics					635:641	waste cotton fabrics	622:641	waste cotton fabrics	622:641	Highly functional cellulose nanofibers (CNF) were produced from waste cotton fabrics and filter paper using an acid hydrolysis technique.					
37047002	0	13	from	Fabrics	91:97	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	0	13	from	Fabrics	91:97	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	0	13	from	Fabrics	91:97	arg1	Membranes					56:64	Functional Biobased Membranes	36:64	Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste	36:112	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	5	14	theme	nanofibers	709:718	arg1	yield					700:704	The yield	696:704	The yield of nanofibers extracted from waste cotton fabrics and filter paper	696:771	The yield of nanofibers extracted from waste cotton fabrics and filter paper was 76.74 and 54.50%, respectively.					
37047002	5	14	theme	nanofibers	709:718	arg1	%					792:792	76.74 and 54.50%	777:792	76.74 and 54.50%	777:792	The yield of nanofibers extracted from waste cotton fabrics and filter paper was 76.74 and 54.50%, respectively.					
37047002	9	15	theme	vacuum	1215:1220	arg1	techniques					1239:1248	vacuum and hot-pressing techniques	1215:1248	techniques	1239:1248	After membrane fabrication using vacuum and hot-pressing techniques, the characteristics of the membrane were analyzed.					
37047002	1	16	theme	colored	215:221	arg1	wastewater					223:232	colored wastewater	215:232	colored wastewater	215:232	Textile industries currently face vast challenges for the active removal of colored wastewater.					
37047002	9	17	theme	hot-pressing	1226:1237	arg1	techniques					1239:1248	vacuum and hot-pressing techniques	1215:1248	techniques	1239:1248	After membrane fabrication using vacuum and hot-pressing techniques, the characteristics of the membrane were analyzed.					
37047002	10	18	theme	membrane	1366:1373	arg1	nm					1385:1386	1.185 nm	1379:1386	1.185 nm	1379:1386	The results showed that the average pore size of the palm-waste membrane was 1.185 nm, while it was 1.875 nm for membrane from waste cotton fibers and filter paper.					
37047002	10	18	theme	membrane	1366:1373	arg1	size					1343:1346	the average pore size	1326:1346	the average pore size of the palm-waste membrane	1326:1373	The results showed that the average pore size of the palm-waste membrane was 1.185 nm, while it was 1.875 nm for membrane from waste cotton fibers and filter paper.					
37047002	12	19	contain	had	1691:1693	arg2	efficiency					1719:1728	the highest dye removal efficiency	1695:1728	the highest dye removal efficiency	1695:1728	The membrane made from palm waste had the highest dye removal efficiency, and it was 23% for Congo red and 44% for methylene blue.					
37047002	12	19	contain	had	1691:1693	arg1	membrane					1661:1668	The membrane	1657:1668	The membrane made from palm waste	1657:1689	The membrane made from palm waste had the highest dye removal efficiency, and it was 23% for Congo red and 44% for methylene blue.					
37047002	4	20	theme	hydrolysis	674:683	arg1	technique					685:693	an acid hydrolysis technique	666:693	an acid hydrolysis technique	666:693	Highly functional cellulose nanofibers (CNF) were produced from waste cotton fabrics and filter paper using an acid hydrolysis technique.					
37047002	0	21	theme	Postconsumer	71:82	arg1	Fabrics					91:97	Postconsumer Cotton Fabrics	71:97	Postconsumer Cotton Fabrics	71:97	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	0	22	from	Characterization	16:31	arg1	Fabrics					91:97	Postconsumer Cotton Fabrics	71:97	Postconsumer Cotton Fabrics	71:97	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	0	22	from	Characterization	16:31	arg1	Waste					108:112	Palm Waste	103:112	Palm Waste	103:112	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	11	23	theme	red	1473:1475	arg1	solutions					1520:1528	model solutions	1514:1528	model solutions	1514:1528	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	11	23	theme	red	1473:1475	arg1	dyes					1496:1499	Congo red and methylene blue dyes	1467:1499	Congo red and methylene blue dyes	1467:1499	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	7	24	theme	nanofibers	959:968	arg1	properties					936:945	The properties	932:945	The properties of isolated nanofibers	932:968	The properties of isolated nanofibers were almost similar and comparable to those of commercial nanofibers.					
37047002	7	24	theme	nanofibers	959:968	arg1	similar					982:988	similar	982:988	similar	982:988	The properties of isolated nanofibers were almost similar and comparable to those of commercial nanofibers.					
37047002	10	25	from	paper	1460:1464	arg1	nm					1408:1409	1.875 nm	1402:1409	1.875 nm for membrane	1402:1422	The results showed that the average pore size of the palm-waste membrane was 1.185 nm, while it was 1.875 nm for membrane from waste cotton fibers and filter paper.					
37047002	3	26	theme	present	338:344	arg1	study					346:350	the present study	334:350	the present study	334:350	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	0	27	from	Fabrication	0:10	arg1	Fabrics					91:97	Postconsumer Cotton Fabrics	71:97	Postconsumer Cotton Fabrics	71:97	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	0	27	from	Fabrication	0:10	arg1	Waste					108:112	Palm Waste	103:112	Palm Waste	103:112	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	0	28	theme	Palm	103:106	arg1	Waste					108:112	Palm Waste	103:112	Palm Waste	103:112	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	13	29	theme	high-performance	1942:1957	arg1	devices					1977:1983	high-performance liquid filtration devices	1942:1983	high-performance liquid filtration devices for a more sustainable society	1942:2014	This study provides insights into the challenges associated with the use of postconsumer textile and agricultural waste, which can be potentially used in high-performance liquid filtration devices for a more sustainable society.					
37047002	10	30	theme	waste	1429:1433	arg1	fibers					1442:1447	waste cotton fibers	1429:1447	waste cotton fibers	1429:1447	The results showed that the average pore size of the palm-waste membrane was 1.185 nm, while it was 1.875 nm for membrane from waste cotton fibers and filter paper.					
37047002	5	31	theme	cotton	741:746	arg1	fabrics					748:754	waste cotton fabrics	735:754	waste cotton fabrics	735:754	The yield of nanofibers extracted from waste cotton fabrics and filter paper was 76.74 and 54.50%, respectively.					
37047002	3	32	from	utilization	365:375	arg1	fabrics					500:506	post-consumer cotton fabrics	479:506	post-consumer cotton fabrics	479:506	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	3	32	from	utilization	365:375	arg1	waste					517:521	palm waste	512:521	palm waste	512:521	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	8	33	theme	charge	1052:1057	arg1	densities					1059:1067	The surface charge densities	1040:1067	The surface charge densities	1040:1067	The surface charge densities were -94.0, -80.7, and -90.6 mV for the nanofibers of palm waste, cotton fibers, and filter paper, respectively.					
37047002	8	33	theme	charge	1052:1057	arg1	mV					1098:1099	-94.0, -80.7, and -90.6 mV	1074:1099	-94.0, -80.7, and -90.6 mV	1074:1099	The surface charge densities were -94.0, -80.7, and -90.6 mV for the nanofibers of palm waste, cotton fibers, and filter paper, respectively.					
37047002	10	34	theme	filter	1453:1458	arg1	paper					1460:1464	filter paper	1453:1464	filter paper	1453:1464	The results showed that the average pore size of the palm-waste membrane was 1.185 nm, while it was 1.875 nm for membrane from waste cotton fibers and filter paper.					
37047002	0	35	theme	Functional	36:45	arg1	Membranes					56:64	Functional Biobased Membranes	36:64	Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste	36:112	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	12	36	theme	blue	1782:1785	arg1	methylene					1772:1780	methylene blue	1772:1785	methylene blue	1772:1785	The membrane made from palm waste had the highest dye removal efficiency, and it was 23% for Congo red and 44% for methylene blue.					
37047002	0	37	theme	Membranes	56:64	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	0	37	theme	Membranes	56:64	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	6	38	theme	chemical	823:830	arg1	properties					848:857	The physical, chemical, and structural properties	809:857	The physical, chemical, and structural properties of nanofibers	809:871	The physical, chemical, and structural properties of nanofibers were studied using various advanced analytical techniques.					
37047002	3	39	theme	composite	454:462	arg1	membranes					464:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes	380:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste	380:521	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	1	40	theme	wastewater	223:232	arg1	removal					204:210	the active removal	193:210	the active removal of colored wastewater	193:232	Textile industries currently face vast challenges for the active removal of colored wastewater.					
37047002	6	41	theme	physical	813:820	arg1	properties					848:857	The physical, chemical, and structural properties	809:857	The physical, chemical, and structural properties of nanofibers	809:871	The physical, chemical, and structural properties of nanofibers were studied using various advanced analytical techniques.					
37047002	11	42	theme	functional	1570:1579	arg1	groups					1581:1586	available functional groups	1560:1586	available functional groups	1560:1586	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	4	43	theme	filter	647:652	arg1	paper					654:658	filter paper	647:658	filter paper	647:658	Highly functional cellulose nanofibers (CNF) were produced from waste cotton fabrics and filter paper using an acid hydrolysis technique.					
37047002	6	44	theme	analytical	909:918	arg1	techniques					920:929	various advanced analytical techniques	892:929	various advanced analytical techniques	892:929	The physical, chemical, and structural properties of nanofibers were studied using various advanced analytical techniques.					
37047002	11	45	theme	surface	1596:1602	arg1	ζ-potential					1604:1614	the surface ζ-potential	1592:1614	the surface ζ-potential of the membrane frameworks' interaction	1592:1654	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	10	46	theme	average	1330:1336	arg1	nm					1385:1386	1.185 nm	1379:1386	1.185 nm	1379:1386	The results showed that the average pore size of the palm-waste membrane was 1.185 nm, while it was 1.875 nm for membrane from waste cotton fibers and filter paper.					
37047002	10	46	theme	average	1330:1336	arg1	size					1343:1346	the average pore size	1326:1346	the average pore size of the palm-waste membrane	1326:1373	The results showed that the average pore size of the palm-waste membrane was 1.185 nm, while it was 1.875 nm for membrane from waste cotton fibers and filter paper.					
37047002	3	47	theme	cotton	493:498	arg1	fabrics					500:506	post-consumer cotton fabrics	479:506	post-consumer cotton fabrics	479:506	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	8	48	theme	palm	1123:1126	arg1	waste					1128:1132	palm waste	1123:1132	palm waste	1123:1132	The surface charge densities were -94.0, -80.7, and -90.6 mV for the nanofibers of palm waste, cotton fibers, and filter paper, respectively.					
37047002	6	49	theme	various	892:898	arg1	techniques					920:929	various advanced analytical techniques	892:929	various advanced analytical techniques	892:929	The physical, chemical, and structural properties of nanofibers were studied using various advanced analytical techniques.					
37047002	12	50	theme	dye	1707:1709	arg1	efficiency					1719:1728	the highest dye removal efficiency	1695:1728	the highest dye removal efficiency	1695:1728	The membrane made from palm waste had the highest dye removal efficiency, and it was 23% for Congo red and 44% for methylene blue.					
37047002	4	51	theme	cellulose	576:584	arg1	CNF					598:600	CNF	598:600	CNF	598:600	Highly functional cellulose nanofibers (CNF) were produced from waste cotton fabrics and filter paper using an acid hydrolysis technique.					
37047002	4	51	theme	cellulose	576:584	arg1	nanofibers					586:595	Highly functional cellulose nanofibers	558:595	Highly functional cellulose nanofibers (CNF)	558:601	Highly functional cellulose nanofibers (CNF) were produced from waste cotton fabrics and filter paper using an acid hydrolysis technique.					
37047002	3	52	from	fabrics	500:506	arg1	utilization					365:375	the utilization	361:375	the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes	361:555	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	3	52	from	fabrics	500:506	arg1	membranes					464:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes	380:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste	380:521	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	3	53	theme	wastewater	527:536	arg1	purposes					548:555	wastewater treatment purposes	527:555	wastewater treatment purposes	527:555	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	11	54	theme	membrane	1623:1630	arg1	frameworks					1632:1641	membrane frameworks	1623:1641	the membrane frameworks' interaction	1619:1654	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	13	55	theme	agricultural	1889:1900	arg1	waste					1902:1906	postconsumer textile and agricultural waste	1864:1906	postconsumer textile and agricultural waste	1864:1906	This study provides insights into the challenges associated with the use of postconsumer textile and agricultural waste, which can be potentially used in high-performance liquid filtration devices for a more sustainable society.					
37047002	1	56	theme	vast	173:176	arg1	challenges					178:187	vast challenges	173:187	vast challenges	173:187	Textile industries currently face vast challenges for the active removal of colored wastewater.					
37047002	8	57	theme	fibers	1142:1147	arg1	nanofibers					1109:1118	the nanofibers	1105:1118	the nanofibers of palm waste, cotton fibers, and filter paper	1105:1165	The surface charge densities were -94.0, -80.7, and -90.6 mV for the nanofibers of palm waste, cotton fibers, and filter paper, respectively.					
37047002	6	58	theme	nanofibers	862:871	arg1	properties					848:857	The physical, chemical, and structural properties	809:857	The physical, chemical, and structural properties of nanofibers	809:871	The physical, chemical, and structural properties of nanofibers were studied using various advanced analytical techniques.					
37047002	2	59	dep	sustainable	243:253	arg1	approaches					278:287	approaches	278:287	approaches	278:287	Indeed, sustainable, recyclable, and green approaches are still lacking to achieve this aim.					
37047002	0	60	theme	Dyes	133:136	arg1	Removal					122:128	the Removal	118:128	the Removal of Dyes	118:136	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	11	61	theme	model	1514:1518	arg1	solutions					1520:1528	model solutions	1514:1528	model solutions	1514:1528	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	11	61	theme	model	1514:1518	arg1	dyes					1496:1499	Congo red and methylene blue dyes	1467:1499	Congo red and methylene blue dyes	1467:1499	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	9	62	theme	membrane	1188:1195	arg1	fabrication					1197:1207	membrane fabrication	1188:1207	membrane fabrication using vacuum and hot-pressing techniques	1188:1248	After membrane fabrication using vacuum and hot-pressing techniques, the characteristics of the membrane were analyzed.					
37047002	1	63	theme	active	197:202	arg1	removal					204:210	the active removal	193:210	the active removal of colored wastewater	193:232	Textile industries currently face vast challenges for the active removal of colored wastewater.					
37047002	8	64	theme	paper	1161:1165	arg1	nanofibers					1109:1118	the nanofibers	1105:1118	the nanofibers of palm waste, cotton fibers, and filter paper	1105:1165	The surface charge densities were -94.0, -80.7, and -90.6 mV for the nanofibers of palm waste, cotton fibers, and filter paper, respectively.					
37047002	4	65	theme	waste	622:626	arg1	fabrics					635:641	waste cotton fabrics	622:641	waste cotton fabrics	622:641	Highly functional cellulose nanofibers (CNF) were produced from waste cotton fabrics and filter paper using an acid hydrolysis technique.					
37047002	13	66	used	used	1934:1937	arg2	waste					1902:1906	postconsumer textile and agricultural waste	1864:1906	postconsumer textile and agricultural waste	1864:1906	This study provides insights into the challenges associated with the use of postconsumer textile and agricultural waste, which can be potentially used in high-performance liquid filtration devices for a more sustainable society.					
37047002	7	67	theme	commercial	1017:1026	arg1	nanofibers					1028:1037	commercial nanofibers	1017:1037	commercial nanofibers	1017:1037	The properties of isolated nanofibers were almost similar and comparable to those of commercial nanofibers.					
37047002	10	68	from	fibers	1442:1447	arg1	nm					1408:1409	1.875 nm	1402:1409	1.875 nm for membrane	1402:1422	The results showed that the average pore size of the palm-waste membrane was 1.185 nm, while it was 1.875 nm for membrane from waste cotton fibers and filter paper.					
37047002	0	69	from	Waste	108:112	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	0	69	from	Waste	108:112	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	0	69	from	Waste	108:112	arg1	Membranes					56:64	Functional Biobased Membranes	36:64	Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste	36:112	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	0	70	theme	Cotton	84:89	arg1	Fabrics					91:97	Postconsumer Cotton Fabrics	71:97	Postconsumer Cotton Fabrics	71:97	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	1	71	theme	Textile	139:145	arg1	industries					147:156	Textile industries	139:156	Textile industries	139:156	Textile industries currently face vast challenges for the active removal of colored wastewater.					
37047002	4	72	theme	acid	669:672	arg1	technique					685:693	an acid hydrolysis technique	666:693	an acid hydrolysis technique	666:693	Highly functional cellulose nanofibers (CNF) were produced from waste cotton fabrics and filter paper using an acid hydrolysis technique.					
37047002	3	73	from	waste	517:521	arg1	utilization					365:375	the utilization	361:375	the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes	361:555	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	3	73	from	waste	517:521	arg1	membranes					464:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes	380:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste	380:521	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	5	74	theme	filter	760:765	arg1	paper					767:771	filter paper	760:771	filter paper	760:771	The yield of nanofibers extracted from waste cotton fabrics and filter paper was 76.74 and 54.50%, respectively.					
37047002	9	75	theme	membrane	1278:1285	arg1	characteristics					1255:1269	the characteristics	1251:1269	the characteristics of the membrane	1251:1285	After membrane fabrication using vacuum and hot-pressing techniques, the characteristics of the membrane were analyzed.					
37047002	8	76	theme	surface	1044:1050	arg1	densities					1059:1067	The surface charge densities	1040:1067	The surface charge densities	1040:1067	The surface charge densities were -94.0, -80.7, and -90.6 mV for the nanofibers of palm waste, cotton fibers, and filter paper, respectively.					
37047002	8	76	theme	surface	1044:1050	arg1	mV					1098:1099	-94.0, -80.7, and -90.6 mV	1074:1099	-94.0, -80.7, and -90.6 mV	1074:1099	The surface charge densities were -94.0, -80.7, and -90.6 mV for the nanofibers of palm waste, cotton fibers, and filter paper, respectively.					
37047002	13	77	theme	waste	1902:1906	arg1	use					1857:1859	the use	1853:1859	the use of postconsumer textile and agricultural waste, which can be potentially used in high-performance liquid filtration devices for a more sustainable society	1853:2014	This study provides insights into the challenges associated with the use of postconsumer textile and agricultural waste, which can be potentially used in high-performance liquid filtration devices for a more sustainable society.					
37047002	5	78	theme	waste	735:739	arg1	fabrics					748:754	waste cotton fabrics	735:754	waste cotton fabrics	735:754	The yield of nanofibers extracted from waste cotton fabrics and filter paper was 76.74 and 54.50%, respectively.					
37047002	13	79	theme	liquid	1959:1964	arg1	devices					1977:1983	high-performance liquid filtration devices	1942:1983	high-performance liquid filtration devices for a more sustainable society	1942:2014	This study provides insights into the challenges associated with the use of postconsumer textile and agricultural waste, which can be potentially used in high-performance liquid filtration devices for a more sustainable society.					
37047002	7	80	theme	isolated	950:957	arg1	nanofibers					959:968	isolated nanofibers	950:968	isolated nanofibers	950:968	The properties of isolated nanofibers were almost similar and comparable to those of commercial nanofibers.					
37047002	3	81	dep	functional	387:396	arg1	bio-based					424:432	bio-based	424:432	bio-based	424:432	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	13	82	theme	textile	1877:1883	arg1	waste					1902:1906	postconsumer textile and agricultural waste	1864:1906	postconsumer textile and agricultural waste	1864:1906	This study provides insights into the challenges associated with the use of postconsumer textile and agricultural waste, which can be potentially used in high-performance liquid filtration devices for a more sustainable society.					
37047002	3	83	theme	recyclable	406:415	arg1	membranes					464:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes	380:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste	380:521	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	4	84	theme	functional	565:574	arg1	CNF					598:600	CNF	598:600	CNF	598:600	Highly functional cellulose nanofibers (CNF) were produced from waste cotton fabrics and filter paper using an acid hydrolysis technique.					
37047002	4	84	theme	functional	565:574	arg1	nanofibers					586:595	Highly functional cellulose nanofibers	558:595	Highly functional cellulose nanofibers (CNF)	558:601	Highly functional cellulose nanofibers (CNF) were produced from waste cotton fabrics and filter paper using an acid hydrolysis technique.					
37047002	3	85	theme	cost-effective	439:452	arg1	membranes					464:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes	380:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste	380:521	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	0	86	theme	Biobased	47:54	arg1	Membranes					56:64	Functional Biobased Membranes	36:64	Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste	36:112	Fabrication and Characterization of Functional Biobased Membranes from Postconsumer Cotton Fabrics and Palm Waste for the Removal of Dyes.					
37047002	6	87	theme	structural	837:846	arg1	properties					848:857	The physical, chemical, and structural properties	809:857	The physical, chemical, and structural properties of nanofibers	809:871	The physical, chemical, and structural properties of nanofibers were studied using various advanced analytical techniques.					
37047002	3	88	theme	green	399:403	arg1	membranes					464:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes	380:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste	380:521	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	10	89	theme	cotton	1435:1440	arg1	fibers					1442:1447	waste cotton fibers	1429:1447	waste cotton fibers	1429:1447	The results showed that the average pore size of the palm-waste membrane was 1.185 nm, while it was 1.875 nm for membrane from waste cotton fibers and filter paper.					
37047002	13	90	theme	filtration	1966:1975	arg1	devices					1977:1983	high-performance liquid filtration devices	1942:1983	high-performance liquid filtration devices for a more sustainable society	1942:2014	This study provides insights into the challenges associated with the use of postconsumer textile and agricultural waste, which can be potentially used in high-performance liquid filtration devices for a more sustainable society.					
37047002	3	91	theme	functional	387:396	arg1	membranes					464:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes	380:472	highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste	380:521	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	3	92	theme	post-consumer	479:491	arg1	fabrics					500:506	post-consumer cotton fabrics	479:506	post-consumer cotton fabrics	479:506	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	11	93	theme	available	1560:1568	arg1	groups					1581:1586	available functional groups	1560:1586	available functional groups	1560:1586	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	3	94	theme	membranes	464:472	arg1	utilization					365:375	the utilization	361:375	the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes	361:555	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
37047002	11	95	used	used	1506:1509	arg2	solutions					1520:1528	model solutions	1514:1528	model solutions	1514:1528	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	11	95	used	used	1506:1509	arg2	dyes					1496:1499	Congo red and methylene blue dyes	1467:1499	Congo red and methylene blue dyes	1467:1499	Congo red and methylene blue dyes were used as model solutions to understand the behavior of available functional groups and the surface ζ-potential of the membrane frameworks' interaction.					
37047002	10	96	theme	pore	1338:1341	arg1	nm					1385:1386	1.185 nm	1379:1386	1.185 nm	1379:1386	The results showed that the average pore size of the palm-waste membrane was 1.185 nm, while it was 1.875 nm for membrane from waste cotton fibers and filter paper.					
37047002	10	96	theme	pore	1338:1341	arg1	size					1343:1346	the average pore size	1326:1346	the average pore size of the palm-waste membrane	1326:1373	The results showed that the average pore size of the palm-waste membrane was 1.185 nm, while it was 1.875 nm for membrane from waste cotton fibers and filter paper.					
37047002	3	97	theme	palm	512:515	arg1	waste					517:521	palm waste	512:521	palm waste	512:521	Thus, the present study explored the utilization of highly functional, green, recyclable, fully bio-based, and cost-effective composite membranes from post-consumer cotton fabrics and palm waste for wastewater treatment purposes.					
35233948	2	0	from	efficiency	469:478	arg1	manner					532:537	acid-controlled manner	516:537	acid-controlled manner	516:537	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	6	1	theme	pesticide	1845:1853	arg1	spots					1828:1832	the spots	1824:1832	the spots of applied pesticide	1824:1853	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	4	2	theme	pristine	1141:1148	arg1	chitosan					1150:1157	pristine chitosan	1141:1157	pristine chitosan	1141:1157	Under the irradiation of UV light (365 nm), the modified chitosan would undergo a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility.					
35233948	5	3	theme	release	1312:1318	arg1	performance					1320:1330	the pesticide release performance	1298:1330	the pesticide release performance	1298:1330	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	2	4	theme	acid	714:717	arg1	input					705:709	the input	701:709	the input of acid	701:717	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	5	5	theme	pH	1589:1590	arg1	change					1592:1597	no obvious pH change	1578:1597	no obvious pH change for the two other situations	1578:1626	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	5	6	theme	attractive	1643:1652	arg1	behavior					1698:1705	an attractive UV light-controlled, acid-propelled release behavior	1640:1705	behavior	1698:1705	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	2	7	theme	Acid-responsive	350:364	arg1	type					381:384	a type	379:384	a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy	379:744	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	2	7	theme	Acid-responsive	350:364	arg1	pesticide					366:374	Acid-responsive pesticide	350:374	Acid-responsive pesticide	350:374	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	4	8	theme	labile	1177:1182	arg1	protons					1184:1190	the labile protons	1173:1190	the labile protons	1173:1190	Under the irradiation of UV light (365 nm), the modified chitosan would undergo a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility.					
35233948	5	9	theme	light-controlled	1657:1672	arg1	behavior					1698:1705	an attractive UV light-controlled, acid-propelled release behavior	1640:1705	behavior	1698:1705	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	0	10	theme	acid	152:155	arg1	generation					138:147	in situ photo-induced generation	116:147	in situ photo-induced generation of acid	116:155	Chitosan-based organic/inorganic composite engineered for UV light-controlled smart pH-responsive pesticide through in situ photo-induced generation of acid.					
35233948	4	11	theme	UV	1040:1041	arg1	365 nm					1050:1055	365 nm	1050:1055	365 nm	1050:1055	Under the irradiation of UV light (365 nm), the modified chitosan would undergo a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility.					
35233948	4	11	theme	UV	1040:1041	arg1	light					1043:1047	UV light	1040:1047	UV light (365 nm)	1040:1056	Under the irradiation of UV light (365 nm), the modified chitosan would undergo a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility.					
35233948	6	12	theme	contamination	1887:1899	arg1	problem					1871:1877	the problem	1867:1877	the problem of acid contamination	1867:1899	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	3	13	theme	acid	867:870	arg1	self-supplier					872:884	a light-maneuvered acid self-supplier	848:884	a light-maneuvered acid self-supplier	848:884	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	4	14	dep	light	1043:1047	arg1	irradiation					1025:1035	irradiation	1025:1035	irradiation	1025:1035	Under the irradiation of UV light (365 nm), the modified chitosan would undergo a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility.					
35233948	4	14	dep	light	1043:1047	arg1	the					1021:1023	the	1021:1023	the	1021:1023	Under the irradiation of UV light (365 nm), the modified chitosan would undergo a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility.					
35233948	4	15	theme	pH-responsive	1258:1270	arg1	flexibility					1272:1282	its inherent pH-responsive flexibility	1245:1282	its inherent pH-responsive flexibility	1245:1282	Under the irradiation of UV light (365 nm), the modified chitosan would undergo a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility.					
35233948	6	16	theme	acid-responsive	2000:2014	arg1	pesticides					2016:2025	acid-responsive pesticides	2000:2025	acid-responsive pesticides	2000:2025	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	5	17	theme	pesticide	1302:1310	arg1	performance					1320:1330	the pesticide release performance	1298:1330	the pesticide release performance	1298:1330	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	2	18	theme	acidic	569:574	arg1	solutions					576:584	acidic solutions	569:584	acidic solutions	569:584	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	1	19	theme	ecological	292:301	arg1	microbalance					303:314	the ecological microbalance	288:314	the ecological microbalance among different species of lives	288:347	BACKGROUND Confined by the volatile property, pesticides are overused and lost significantly during and after spraying, weakening the ecological microbalance among different species of lives.					
35233948	6	20	theme	proposed	1763:1770	arg1	generation					1794:1803	the proposed in situ photo-induced generation	1759:1803	the proposed in situ photo-induced generation of acid	1759:1811	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	6	20	theme	proposed	1763:1770	arg1	approach					1749:1756	direct acid spray approach	1731:1756	direct acid spray approach	1731:1756	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	2	21	from	ingredients	501:511	arg1	manner					532:537	acid-controlled manner	516:537	acid-controlled manner	516:537	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	5	22	theme	consistent	1519:1528	arg1	time					1513:1516	the same time	1504:1516	the same time	1504:1516	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	6	23	theme	spray	1743:1747	arg1	generation					1794:1803	the proposed in situ photo-induced generation	1759:1803	the proposed in situ photo-induced generation of acid	1759:1811	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	6	23	theme	spray	1743:1747	arg1	approach					1749:1756	direct acid spray approach	1731:1756	direct acid spray approach	1731:1756	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	5	24	theme	acid-propelled	1675:1688	arg1	behavior					1698:1705	an attractive UV light-controlled, acid-propelled release behavior	1640:1705	behavior	1698:1705	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	3	25	theme	insecticide	989:999	arg1	chlorpyrifos					1001:1012	attapulgite and organophosphate insecticide chlorpyrifos	957:1012	attapulgite and organophosphate insecticide chlorpyrifos	957:1012	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	6	26	theme	direct	1731:1736	arg1	generation					1794:1803	the proposed in situ photo-induced generation	1759:1803	the proposed in situ photo-induced generation of acid	1759:1811	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	6	26	theme	direct	1731:1736	arg1	approach					1749:1756	direct acid spray approach	1731:1756	direct acid spray approach	1731:1756	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	6	27	theme	management	2036:2045	arg1	delivery					1988:1995	the controlled delivery	1973:1995	the controlled delivery of acid-responsive pesticides and pest management	1973:2045	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	0	28	theme	Chitosan-based	0:13	arg1	composite					33:41	Chitosan-based organic/inorganic composite	0:41	Chitosan-based organic/inorganic composite engineered for UV	0:59	Chitosan-based organic/inorganic composite engineered for UV light-controlled smart pH-responsive pesticide through in situ photo-induced generation of acid.					
35233948	2	29	theme	active	494:499	arg1	ingredients					501:511	pesticidal active ingredients	483:511	pesticidal active ingredients in acid-controlled manner	483:537	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	6	30	theme	acid	1808:1811	arg1	generation					1794:1803	the proposed in situ photo-induced generation	1759:1803	the proposed in situ photo-induced generation of acid	1759:1811	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	6	30	theme	acid	1808:1811	arg1	approach					1749:1756	direct acid spray approach	1731:1756	direct acid spray approach	1731:1756	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	5	31	theme	UV	1565:1566	arg1	light					1568:1572	UV light	1565:1572	UV light	1565:1572	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	2	32	theme	utilization	457:467	arg1	efficiency					469:478	utilization efficiency	457:478	utilization efficiency of pesticidal active ingredients in acid-controlled manner	457:537	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	5	33	theme	release	1690:1696	arg1	behavior					1698:1705	an attractive UV light-controlled, acid-propelled release behavior	1640:1705	behavior	1698:1705	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	1	34	theme	lives	343:347	arg1	species					332:338	different species	322:338	different species of lives	322:347	BACKGROUND Confined by the volatile property, pesticides are overused and lost significantly during and after spraying, weakening the ecological microbalance among different species of lives.					
35233948	4	35	theme	photolytic	1097:1106	arg1	reaction					1108:1115	a photolytic reaction	1095:1115	a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility	1095:1282	Under the irradiation of UV light (365 nm), the modified chitosan would undergo a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility.					
35233948	0	36	theme	pH-responsive	84:96	arg1	pesticide					98:106	smart pH-responsive pesticide	78:106	smart pH-responsive pesticide	78:106	Chitosan-based organic/inorganic composite engineered for UV light-controlled smart pH-responsive pesticide through in situ photo-induced generation of acid.					
35233948	2	37	theme	engineered	397:406	arg1	pesticides					408:417	smartly engineered pesticides	389:417	smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy	389:744	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	6	38	theme	applied	1837:1843	arg1	pesticide					1845:1853	applied pesticide	1837:1853	applied pesticide	1837:1853	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	3	39	dep	RESULTS	747:753	arg1	modified					779:786	modified	779:786	was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos	764:1012	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	2	40	theme	secondary	667:675	arg1	pollution					677:685	secondary pollution	667:685	secondary pollution	667:685	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	5	41	theme	obvious	1581:1587	arg1	change					1592:1597	no obvious pH change	1578:1597	no obvious pH change for the two other situations	1578:1626	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	7	42	theme	Chemical	2066:2073	arg1	Industry					2075:2082	Chemical Industry	2066:2082	Chemical Industry	2066:2082	© 2022 Society of Chemical Industry.					
35233948	4	43	theme	chlorpyrifos	1223:1234	arg1	release					1212:1218	the release	1208:1218	the release of chlorpyrifos based on its inherent pH-responsive flexibility	1208:1282	Under the irradiation of UV light (365 nm), the modified chitosan would undergo a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility.					
35233948	3	44	theme	photoacid	795:803	arg1	2-nitrobenzaldehyde					816:834	2-nitrobenzaldehyde	816:834	2-nitrobenzaldehyde	816:834	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	3	44	theme	photoacid	795:803	arg1	generator					805:813	a photoacid generator	793:813	a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos	793:1012	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	5	45	theme	UV	1654:1655	arg1	behavior					1698:1705	an attractive UV light-controlled, acid-propelled release behavior	1640:1705	behavior	1698:1705	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	2	46	theme	microenvironment	613:628	arg1	balance					602:608	the balance	598:608	the balance of microenvironment surrounding targeted plants	598:656	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	6	47	dep	higher	1930:1935	arg1	efficiency					1937:1946	efficiency	1937:1946	efficiency	1937:1946	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	5	48	dep	%	1459:1459	arg1	4.2 mW/cm2					1462:1471	4.2 mW/cm2	1462:1471	4.2 mW/cm2	1462:1471	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	5	49	theme	other	1611:1615	arg1	situations					1617:1626	the two other situations	1603:1626	the two other situations	1603:1626	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	6	50	theme	acid	1882:1885	arg1	contamination					1887:1899	acid contamination	1882:1899	acid contamination	1882:1899	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	5	51	theme	UV	1372:1373	arg1	light					1375:1379	UV light	1372:1379	UV light (27.2 mW/cm2 )	1372:1394	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	5	51	theme	UV	1372:1373	arg1	27.2 mW/cm2					1382:1392	27.2 mW/cm2	1382:1392	27.2 mW/cm2	1382:1392	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	3	52	theme	light-maneuvered	850:865	arg1	self-supplier					872:884	a light-maneuvered acid self-supplier	848:884	a light-maneuvered acid self-supplier	848:884	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	2	53	theme	targeted	642:649	arg1	plants					651:656	targeted plants	642:656	targeted plants	642:656	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	6	54	theme	controlled	1977:1986	arg1	delivery					1988:1995	the controlled delivery	1973:1995	the controlled delivery of acid-responsive pesticides and pest management	1973:2045	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	3	55	mod	modified	779:786	arg3	2-nitrobenzaldehyde					816:834	2-nitrobenzaldehyde	816:834	2-nitrobenzaldehyde	816:834	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	3	55	mod	modified	779:786	arg3	generator					805:813	a photoacid generator	793:813	a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos	793:1012	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	3	55	mod	modified	779:786	arg1	Chitosan					755:762	Chitosan	755:762	Chitosan	755:762	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	5	56	with	consistent	1519:1528	arg1	reduction					1542:1550	the pH reduction	1535:1550	the pH reduction to 5.3 under UV light	1535:1572	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	5	56	with	consistent	1519:1528	arg1	change					1592:1597	no obvious pH change	1578:1597	no obvious pH change for the two other situations	1578:1626	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	6	57	theme	in	1772:1773	arg1	generation					1794:1803	the proposed in situ photo-induced generation	1759:1803	the proposed in situ photo-induced generation of acid	1759:1811	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	6	57	theme	in	1772:1773	arg1	approach					1749:1756	direct acid spray approach	1731:1756	direct acid spray approach	1731:1756	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	4	58	theme	inherent	1249:1256	arg1	flexibility					1272:1282	its inherent pH-responsive flexibility	1245:1282	its inherent pH-responsive flexibility	1245:1282	Under the irradiation of UV light (365 nm), the modified chitosan would undergo a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility.					
35233948	0	59	theme	in	116:117	arg1	generation					138:147	in situ photo-induced generation	116:147	in situ photo-induced generation of acid	116:155	Chitosan-based organic/inorganic composite engineered for UV light-controlled smart pH-responsive pesticide through in situ photo-induced generation of acid.					
35233948	1	60	theme	volatile	185:192	arg1	pesticides					204:213	pesticides	204:213	pesticides	204:213	BACKGROUND Confined by the volatile property, pesticides are overused and lost significantly during and after spraying, weakening the ecological microbalance among different species of lives.					
35233948	1	60	theme	volatile	185:192	arg1	property					194:201	the volatile property	181:201	the volatile property	181:201	BACKGROUND Confined by the volatile property, pesticides are overused and lost significantly during and after spraying, weakening the ecological microbalance among different species of lives.					
35233948	3	61	theme	organophosphate	973:987	arg1	chlorpyrifos					1001:1012	attapulgite and organophosphate insecticide chlorpyrifos	957:1012	attapulgite and organophosphate insecticide chlorpyrifos	957:1012	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	4	62	theme	modified	1063:1070	arg1	chitosan					1072:1079	the modified chitosan	1059:1079	the modified chitosan	1059:1079	Under the irradiation of UV light (365 nm), the modified chitosan would undergo a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility.					
35233948	2	63	theme	precise	729:735	arg1	strategy					737:744	a more precise strategy	722:744	a more precise strategy	722:744	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	5	64	theme	chlorpyrifos	1353:1364	arg1	rate					1345:1348	the release rate	1333:1348	the release rate of chlorpyrifos under UV light (27.2 mW/cm2 )	1333:1394	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	0	65	theme	photo-induced	124:136	arg1	generation					138:147	in situ photo-induced generation	116:147	in situ photo-induced generation of acid	116:155	Chitosan-based organic/inorganic composite engineered for UV light-controlled smart pH-responsive pesticide through in situ photo-induced generation of acid.					
35233948	3	66	theme	attapulgite	957:967	arg1	chlorpyrifos					1001:1012	attapulgite and organophosphate insecticide chlorpyrifos	957:1012	attapulgite and organophosphate insecticide chlorpyrifos	957:1012	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	6	67	dep	in	1772:1773	arg1	situ					1775:1778	situ	1775:1778	situ	1775:1778	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	2	68	from	manner	532:537	arg1	efficiency					469:478	utilization efficiency	457:478	utilization efficiency of pesticidal active ingredients in acid-controlled manner	457:537	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	3	69	theme	smart	904:908	arg1	pesticide					910:918	a smart pesticide	902:918	a smart pesticide	902:918	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	4	70	dep	acid	1132:1135	arg1	an					1129:1130	an	1129:1130	an	1129:1130	Under the irradiation of UV light (365 nm), the modified chitosan would undergo a photolytic reaction to generate an acid and pristine chitosan, which seized the labile protons and facilitated the release of chlorpyrifos based on its inherent pH-responsive flexibility.					
35233948	5	71	from	sunlight	1447:1454	arg1	dark					1486:1489	the dark	1482:1489	the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior	1482:1705	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	5	71	from	sunlight	1447:1454	arg1	%					1494:1494	20%	1492:1494	20%	1492:1494	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	2	72	theme	solutions	576:584	arg1	implementation					551:564	the implementation	547:564	the implementation of acidic solutions	547:584	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	6	73	theme	acid	1738:1741	arg1	generation					1794:1803	the proposed in situ photo-induced generation	1759:1803	the proposed in situ photo-induced generation of acid	1759:1811	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	6	73	theme	acid	1738:1741	arg1	approach					1749:1756	direct acid spray approach	1731:1756	direct acid spray approach	1731:1756	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	5	74	theme	higher	1423:1428	arg1	%					1406:1406	78%	1404:1406	78%	1404:1406	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	0	75	theme	organic/inorganic	15:31	arg1	composite					33:41	Chitosan-based organic/inorganic composite	0:41	Chitosan-based organic/inorganic composite engineered for UV	0:59	Chitosan-based organic/inorganic composite engineered for UV light-controlled smart pH-responsive pesticide through in situ photo-induced generation of acid.					
35233948	0	76	dep	in	116:117	arg1	situ					119:122	situ	119:122	situ	119:122	Chitosan-based organic/inorganic composite engineered for UV light-controlled smart pH-responsive pesticide through in situ photo-induced generation of acid.					
35233948	3	77	theme	chlorpyrifos	1001:1012	arg1	integration					942:952	the integration	938:952	the integration of attapulgite and organophosphate insecticide chlorpyrifos	938:1012	RESULTS Chitosan was chemically modified with a photoacid generator (2-nitrobenzaldehyde) serving as a light-maneuvered acid self-supplier, based on which a smart pesticide was formulated by the integration of attapulgite and organophosphate insecticide chlorpyrifos.					
35233948	2	78	theme	ingredients	501:511	arg1	efficiency					469:478	utilization efficiency	457:478	utilization efficiency of pesticidal active ingredients in acid-controlled manner	457:537	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	5	79	theme	same	1508:1511	arg1	time					1513:1516	the same time	1504:1516	the same time	1504:1516	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	6	80	theme	pest	2031:2034	arg1	management					2036:2045	pest management	2031:2045	pest management	2031:2045	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	1	81	theme	different	322:330	arg1	species					332:338	different species	322:338	different species of lives	322:347	BACKGROUND Confined by the volatile property, pesticides are overused and lost significantly during and after spraying, weakening the ecological microbalance among different species of lives.					
35233948	2	82	theme	pesticidal	483:492	arg1	ingredients					501:511	pesticidal active ingredients	483:511	pesticidal active ingredients in acid-controlled manner	483:537	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	6	83	theme	pesticides	2016:2025	arg1	delivery					1988:1995	the controlled delivery	1973:1995	the controlled delivery of acid-responsive pesticides and pest management	1973:2045	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	2	84	theme	efficiency	469:478	arg1	improvement					442:452	the improvement	438:452	the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner	438:537	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	0	85	theme	smart	78:82	arg1	pesticide					98:106	smart pH-responsive pesticide	78:106	smart pH-responsive pesticide	78:106	Chitosan-based organic/inorganic composite engineered for UV light-controlled smart pH-responsive pesticide through in situ photo-induced generation of acid.					
35233948	2	86	theme	acid-controlled	516:530	arg1	manner					532:537	acid-controlled manner	516:537	acid-controlled manner	516:537	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	6	87	theme	photo-induced	1780:1792	arg1	generation					1794:1803	the proposed in situ photo-induced generation	1759:1803	the proposed in situ photo-induced generation of acid	1759:1811	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	6	87	theme	photo-induced	1780:1792	arg1	approach					1749:1756	direct acid spray approach	1731:1756	direct acid spray approach	1731:1756	CONCLUSION Compared to direct acid spray approach, the proposed in situ photo-induced generation of acid locally on the spots of applied pesticide circumvents the problem of acid contamination to nontargets, demonstrating higher efficiency and biocompatibility for the controlled delivery of acid-responsive pesticides and pest management.					
35233948	5	88	theme	pH	1539:1540	arg1	reduction					1542:1550	the pH reduction	1535:1550	the pH reduction to 5.3 under UV light	1535:1572	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35233948	2	89	theme	pesticides	408:417	arg1	type					381:384	a type	379:384	a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy	379:744	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	2	89	theme	pesticides	408:417	arg1	pesticide					366:374	Acid-responsive pesticide	350:374	Acid-responsive pesticide	350:374	Acid-responsive pesticide is a type of smartly engineered pesticides that contribute to the improvement of utilization efficiency of pesticidal active ingredients in acid-controlled manner, whilst the implementation of acidic solutions may disturb the balance of microenvironment surrounding targeted plants or cause secondary pollution, underscoring the input of acid in a more precise strategy.					
35233948	5	90	theme	release	1337:1343	arg1	rate					1345:1348	the release rate	1333:1348	the release rate of chlorpyrifos under UV light (27.2 mW/cm2 )	1333:1394	According to the pesticide release performance, the release rate of chlorpyrifos under UV light (27.2 mW/cm2 ) reached 78%, significantly higher than those under sunlight (22%, 4.2 mW/cm2 ) and in the dark (20%) within the same time, consistent with the pH reduction to 5.3 under UV light and no obvious pH change for the two other situations, exhibiting an attractive UV light-controlled, acid-propelled release behavior.					
35596852	9	0	theme	MRSA	1039:1042	arg1	bacteria					1044:1051	all MRSA bacteria	1035:1051	all MRSA bacteria	1035:1051	Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria.					
35596852	7	1	theme	Releasing	739:747	arg1	enzyme					753:758	Releasing ALP enzyme	739:758	Releasing ALP enzyme from MC3T3 cells	739:775	Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings.					
35596852	10	2	theme	multifunctional	1220:1234	arg1	implant					1241:1247	a multifunctional bone implant	1218:1247	a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity	1218:1318	In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.					
35596852	10	2	theme	multifunctional	1220:1234	arg1	coating					1190:1196	chitosan/bioactive glass/tetracycline/Melittin coating	1143:1196	chitosan/bioactive glass/tetracycline/Melittin coating	1143:1196	In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.					
35596852	1	3	theme	serious	178:184	arg1	hazards					186:192	serious hazards	178:192	serious hazards of bone implants	178:209	Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants.					
35596852	1	3	theme	serious	178:184	arg1	resistance					125:134	Methicillin resistance	113:134	Methicillin resistance Staphylococcus aureus bacteria (MRSA)	113:172	Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants.					
35596852	6	4	theme	bone	693:696	arg1	regeneration					698:709	bone regeneration	693:709	bone regeneration	693:709	Tetracycline in coatings revealed multifunctional behaviors include bone regeneration and antibacterial activity.					
35596852	3	5	theme	nanoparticles/tetracycline	386:411	arg1	coatings					423:430	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings	361:430	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings	361:430	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings were deposited on hydrothermally etched titanium substrate.					
35596852	10	6	theme	glass/tetracycline/Melittin	1162:1188	arg1	coating					1190:1196	chitosan/bioactive glass/tetracycline/Melittin coating	1143:1196	chitosan/bioactive glass/tetracycline/Melittin coating	1143:1196	In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.					
35596852	10	6	theme	glass/tetracycline/Melittin	1162:1188	arg1	implant					1241:1247	a multifunctional bone implant	1218:1247	a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity	1218:1318	In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.					
35596852	7	7	from	agent	868:872	arg1	implants					888:895	orthopedic implants	877:895	orthopedic implants	877:895	Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings.					
35596852	2	8	theme	tetracycline	297:308	arg1	effects					273:279	the potential synergistic effects	247:279	the potential synergistic effects of Melittin and tetracycline	247:308	The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection.					
35596852	8	9	theme	cells	953:957	arg1	proliferation					930:942	the proliferation	926:942	the proliferation of MC3T3 cells	926:957	Melittin increased the proliferation of MC3T3 cells.					
35596852	3	10	theme	composite	413:421	arg1	coatings					423:430	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings	361:430	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings	361:430	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings were deposited on hydrothermally etched titanium substrate.					
35596852	4	11	theme	drop	542:545	arg1	method					555:560	drop casting method	542:560	drop casting method	542:560	Melittin was then coated on composite coatings by drop casting method.					
35596852	3	12	theme	Chitosan/bioactive	361:378	arg1	coatings					423:430	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings	361:430	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings	361:430	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings were deposited on hydrothermally etched titanium substrate.					
35596852	1	13	dep	Staphylococcus	136:149	arg1	aureus					151:156	aureus	151:156	aureus	151:156	Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants.					
35596852	3	14	theme	glass	380:384	arg1	coatings					423:430	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings	361:430	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings	361:430	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings were deposited on hydrothermally etched titanium substrate.					
35596852	3	15	theme	titanium	472:479	arg1	substrate					481:489	hydrothermally etched titanium substrate	450:489	hydrothermally etched titanium substrate	450:489	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings were deposited on hydrothermally etched titanium substrate.					
35596852	0	16	theme	bactericidal	10:21	arg1	performance					23:33	bactericidal performance	10:33	bactericidal performance of implant composite coatings	10:63	Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline.					
35596852	4	17	theme	casting	547:553	arg1	method					555:560	drop casting method	542:560	drop casting method	542:560	Melittin was then coated on composite coatings by drop casting method.					
35596852	2	18	theme	associated	326:335	arg1	infection					350:358	MRSA associated bone implant infection	321:358	MRSA associated bone implant infection	321:358	The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection.					
35596852	1	19	theme	bone	197:200	arg1	implants					202:209	bone implants	197:209	bone implants	197:209	Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants.					
35596852	6	20	theme	multifunctional	659:673	arg1	behaviors					675:683	multifunctional behaviors	659:683	multifunctional behaviors include bone regeneration and antibacterial activity	659:736	Tetracycline in coatings revealed multifunctional behaviors include bone regeneration and antibacterial activity.					
35596852	9	21	dep	t	1095:1095	arg1	no					1092:1093	no	1092:1093	no	1092:1093	Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria.					
35596852	2	22	theme	MRSA	321:324	arg1	infection					350:358	MRSA associated bone implant infection	321:358	MRSA associated bone implant infection	321:358	The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection.					
35596852	1	23	theme	implants	202:209	arg1	hazards					186:192	serious hazards	178:192	serious hazards of bone implants	178:209	Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants.					
35596852	1	23	theme	implants	202:209	arg1	resistance					125:134	Methicillin resistance	113:134	Methicillin resistance Staphylococcus aureus bacteria (MRSA)	113:172	Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants.					
35596852	9	24	theme	them	1081:1084	arg1	them					1081:1084	them	1081:1084	them	1081:1084	Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria.					
35596852	9	24	theme	them	1081:1084	arg1	one					1074:1076	one	1074:1076	one	1074:1076	Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria.					
35596852	9	25	theme	Composite	960:968	arg1	coatings					970:977	Composite coatings	960:977	Composite coatings with combination of tetracycline and Melittin	960:1023	Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria.					
35596852	11	26	dep	abstract	1331:1338	arg1	Graphical					1321:1329	Graphical	1321:1329	Graphical	1321:1329	Graphical abstract.					
35596852	1	27	theme	Methicillin	113:123	arg1	hazards					186:192	serious hazards	178:192	serious hazards of bone implants	178:209	Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants.					
35596852	1	27	theme	Methicillin	113:123	arg1	MRSA					168:171	MRSA	168:171	MRSA	168:171	Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants.					
35596852	1	27	theme	Methicillin	113:123	arg1	resistance					125:134	Methicillin resistance	113:134	Methicillin resistance Staphylococcus aureus bacteria (MRSA)	113:172	Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants.					
35596852	7	28	theme	orthopedic	877:886	arg1	implants					888:895	orthopedic implants	877:895	orthopedic implants	877:895	Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings.					
35596852	2	29	theme	Melittin	284:291	arg1	effects					273:279	the potential synergistic effects	247:279	the potential synergistic effects of Melittin and tetracycline	247:308	The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection.					
35596852	0	30	theme	composite	46:54	arg1	coatings					56:63	implant composite coatings	38:63	implant composite coatings	38:63	Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline.					
35596852	10	31	theme	bone	1236:1239	arg1	implant					1241:1247	a multifunctional bone implant	1218:1247	a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity	1218:1318	In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.					
35596852	10	31	theme	bone	1236:1239	arg1	coating					1190:1196	chitosan/bioactive glass/tetracycline/Melittin coating	1143:1196	chitosan/bioactive glass/tetracycline/Melittin coating	1143:1196	In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.					
35596852	9	32	with	coatings	1060:1067	arg1	them					1081:1084	them	1081:1084	them	1081:1084	Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria.					
35596852	9	32	with	coatings	1060:1067	arg1	one					1074:1076	one	1074:1076	one	1074:1076	Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria.					
35596852	0	33	theme	implant	38:44	arg1	coatings					56:63	implant composite coatings	38:63	implant composite coatings	38:63	Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline.					
35596852	10	34	theme	antibacterial	1297:1309	arg1	activity					1311:1318	its osteogenic and promising antibacterial activity	1268:1318	its osteogenic and promising antibacterial activity	1268:1318	In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.					
35596852	5	35	theme	XRD	599:601	arg1	instruments					612:622	FTIR, XRD, and SEM instruments	593:622	FTIR, XRD, and SEM instruments	593:622	The surfaces were analyzed by FTIR, XRD, and SEM instruments.					
35596852	9	36	with	coatings	970:977	arg1	combination					984:994	combination	984:994	combination of tetracycline and Melittin	984:1023	Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria.					
35596852	7	37	from	cells	771:775	arg1	enzyme					753:758	Releasing ALP enzyme	739:758	Releasing ALP enzyme from MC3T3 cells	739:775	Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings.					
35596852	7	38	dep	osteoinductive	835:848	arg1	coatings					897:904	coatings	897:904	coatings	897:904	Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings.					
35596852	4	39	theme	composite	520:528	arg1	coatings					530:537	composite coatings	520:537	composite coatings	520:537	Melittin was then coated on composite coatings by drop casting method.					
35596852	1	40	theme	Staphylococcus	136:149	arg1	bacteria					158:165	Staphylococcus aureus bacteria	136:165	Methicillin resistance Staphylococcus aureus bacteria (MRSA)	113:172	Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants.					
35596852	0	41	theme	coatings	56:63	arg1	performance					23:33	bactericidal performance	10:33	bactericidal performance of implant composite coatings	10:63	Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline.					
35596852	9	42	theme	tetracycline	999:1010	arg1	combination					984:994	combination	984:994	combination of tetracycline and Melittin	984:1023	Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria.					
35596852	10	43	theme	chitosan/bioactive	1143:1160	arg1	coating					1190:1196	chitosan/bioactive glass/tetracycline/Melittin coating	1143:1196	chitosan/bioactive glass/tetracycline/Melittin coating	1143:1196	In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.					
35596852	10	43	theme	chitosan/bioactive	1143:1160	arg1	implant					1241:1247	a multifunctional bone implant	1218:1247	a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity	1218:1318	In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.					
35596852	7	44	theme	MC3T3	765:769	arg1	cells					771:775	MC3T3 cells	765:775	MC3T3 cells	765:775	Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings.					
35596852	7	45	theme	antibacterial	854:866	arg1	agent					868:872	antibacterial agent	854:872	antibacterial agent in orthopedic implants	854:895	Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings.					
35596852	6	46	dep	behaviors	675:683	arg1	include					685:691	include	685:691	include bone regeneration and antibacterial activity	685:736	Tetracycline in coatings revealed multifunctional behaviors include bone regeneration and antibacterial activity.					
35596852	2	47	theme	present	216:222	arg1	study					224:228	The present study	212:228	The present study	212:228	The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection.					
35596852	5	48	theme	SEM	608:610	arg1	instruments					612:622	FTIR, XRD, and SEM instruments	593:622	FTIR, XRD, and SEM instruments	593:622	The surfaces were analyzed by FTIR, XRD, and SEM instruments.					
35596852	2	49	theme	implant	342:348	arg1	infection					350:358	MRSA associated bone implant infection	321:358	MRSA associated bone implant infection	321:358	The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection.					
35596852	9	50	theme	Melittin	1016:1023	arg1	combination					984:994	combination	984:994	combination of tetracycline and Melittin	984:1023	Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria.					
35596852	7	51	theme	ALP	749:751	arg1	enzyme					753:758	Releasing ALP enzyme	739:758	Releasing ALP enzyme from MC3T3 cells	739:775	Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings.					
35596852	2	52	theme	synergistic	261:271	arg1	effects					273:279	the potential synergistic effects	247:279	the potential synergistic effects of Melittin and tetracycline	247:308	The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection.					
35596852	2	53	theme	bone	337:340	arg1	infection					350:358	MRSA associated bone implant infection	321:358	MRSA associated bone implant infection	321:358	The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection.					
35596852	6	54	from	Tetracycline	625:636	arg1	coatings					641:648	coatings	641:648	coatings	641:648	Tetracycline in coatings revealed multifunctional behaviors include bone regeneration and antibacterial activity.					
35596852	2	55	theme	potential	251:259	arg1	effects					273:279	the potential synergistic effects	247:279	the potential synergistic effects of Melittin and tetracycline	247:308	The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection.					
35596852	3	56	theme	etched	465:470	arg1	substrate					481:489	hydrothermally etched titanium substrate	450:489	hydrothermally etched titanium substrate	450:489	Chitosan/bioactive glass nanoparticles/tetracycline composite coatings were deposited on hydrothermally etched titanium substrate.					
35596852	6	57	theme	antibacterial	715:727	arg1	activity					729:736	antibacterial activity	715:736	antibacterial activity	715:736	Tetracycline in coatings revealed multifunctional behaviors include bone regeneration and antibacterial activity.					
35596852	7	58	theme	suitable	813:820	arg1	candidate					822:830	suitable candidate	813:830	suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings	813:904	Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings.					
35596852	10	59	theme	promising	1287:1295	arg1	activity					1311:1318	its osteogenic and promising antibacterial activity	1268:1318	its osteogenic and promising antibacterial activity	1268:1318	In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.					
35596852	5	60	theme	FTIR	593:596	arg1	instruments					612:622	FTIR, XRD, and SEM instruments	593:622	FTIR, XRD, and SEM instruments	593:622	The surfaces were analyzed by FTIR, XRD, and SEM instruments.					
35596852	1	61	dep	resistance	125:134	arg1	bacteria					158:165	Staphylococcus aureus bacteria	136:165	Methicillin resistance Staphylococcus aureus bacteria (MRSA)	113:172	Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants.					
35596852	8	62	theme	MC3T3	947:951	arg1	cells					953:957	MC3T3 cells	947:957	MC3T3 cells	947:957	Melittin increased the proliferation of MC3T3 cells.					
35596852	10	63	theme	osteogenic	1272:1281	arg1	activity					1311:1318	its osteogenic and promising antibacterial activity	1268:1318	its osteogenic and promising antibacterial activity	1268:1318	In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.					
36771173	8	0	theme	GAE	1447:1449	arg1	g-1					1451:1453	>0.5 mg GAE g-1	1439:1453	>0.5 mg GAE g-1	1439:1453	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	8	0	theme	GAE	1447:1449	arg1	content					1430:1436	highest equivalent polyphenols content	1399:1436	highest equivalent polyphenols content (>0.5 mg GAE g-1)	1399:1454	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	9	1	with	Films	1457:1461	arg1	particles					1474:1482	particles	1474:1482	particles	1474:1482	Films with 2% of particles were not significantly different.					
36771173	9	1	with	Films	1457:1461	arg1	%					1469:1469	2%	1468:1469	2% of particles	1468:1482	Films with 2% of particles were not significantly different.					
36771173	4	2	theme	Scanning	653:660	arg1	microscopy					671:680	Scanning electron microscopy	653:680	Scanning electron microscopy	653:680	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	3	3	theme	eutectic	546:553	arg1	solvent					555:561	a deep eutectic solvent	539:561	a deep eutectic solvent (DES)	539:567	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	3	3	theme	eutectic	546:553	arg1	films					437:441	Chitosan films	428:441	Chitosan films	428:441	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	3	3	theme	eutectic	546:553	arg1	DES					564:566	DES	564:566	DES	564:566	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	1	4	theme	packaging	197:205	arg1	development					154:164	The development	150:164	The development of biobased antioxidant active packaging	150:205	The development of biobased antioxidant active packaging has been valued by the food industry for complying with environmental and food waste concerns.					
36771173	9	5	theme	particles	1474:1482	arg1	particles					1474:1482	particles	1474:1482	particles	1474:1482	Films with 2% of particles were not significantly different.					
36771173	9	5	theme	particles	1474:1482	arg1	%					1469:1469	2%	1468:1469	2% of particles	1468:1482	Films with 2% of particles were not significantly different.					
36771173	2	6	theme	physicochemical	316:330	arg1	properties					332:341	physicochemical properties	316:341	physicochemical properties for chitosan composite films as a potential active food packaging	316:407	In this work, physicochemical properties for chitosan composite films as a potential active food packaging were investigated.					
36771173	2	7	theme	potential	377:385	arg1	packaging					399:407	a potential active food packaging	375:407	a potential active food packaging	375:407	In this work, physicochemical properties for chitosan composite films as a potential active food packaging were investigated.					
36771173	6	8	theme	5	1116:1116	arg1	%					1117:1117	%	1117:1117	%	1117:1117	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	0	9	theme	Açaí-Filled	123:133	arg1	Microcapsules					135:147	Açaí-Filled Microcapsules	123:147	Açaí-Filled Microcapsules	123:147	Improved Antioxidant and Mechanical Properties of Food Packaging Films Based on Chitosan/Deep Eutectic Solvent, Containing Açaí-Filled Microcapsules.					
36771173	8	10	dep	equivalent	1407:1416	arg1	highest					1399:1405	highest	1399:1405	highest	1399:1405	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	5	11	contain	had	842:844	arg1	content					834:840	The increased PECs content	815:840	The increased PECs content	815:840	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	5	11	contain	had	842:844	arg2	influence					858:866	significant influence	846:866	significant influence	846:866	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	6	12	theme	following	1063:1071	arg1	order					1073:1077	the following order	1059:1077	the following order	1059:1077	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	6	13	dep	MPa	1131:1133	arg1	DES					1139:1141	5% DES	1136:1141	5% DES	1136:1141	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	6	13	dep	MPa	1131:1133	arg1	PECs					1150:1153	5% PECs	1147:1153	5% PECs	1147:1153	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	4	14	contain	contained	784:792	arg1	films					758:762	the chitosan composite films	735:762	the chitosan composite films	735:762	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	4	14	contain	contained	784:792	arg2	PECs					809:812	well-dispersed PECs	794:812	well-dispersed PECs	794:812	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	4	14	contain	contained	784:792	arg1	continuous					769:778	continuous	769:778	continuous	769:778	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	1	15	theme	food	230:233	arg1	industry					235:242	the food industry	226:242	the food industry	226:242	The development of biobased antioxidant active packaging has been valued by the food industry for complying with environmental and food waste concerns.					
36771173	8	16	theme	highest	1292:1298	arg1	properties					1312:1321	the highest antioxidant properties	1288:1321	the highest antioxidant properties against the ABTS	1288:1338	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	6	17	theme	5	1147:1147	arg1	%					1148:1148	%	1148:1148	%	1148:1148	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	10	18	theme	microparticles	1592:1605	arg1	microparticles					1592:1605	microparticles	1592:1605	microparticles	1592:1605	These results suggested that the chitosan films that incorporated 1-2% of microparticles had the best combined mechanical and antioxidant properties as a potential material for food packaging.					
36771173	10	18	theme	microparticles	1592:1605	arg1	%					1587:1587	1-2%	1584:1587	1-2% of microparticles	1584:1605	These results suggested that the chitosan films that incorporated 1-2% of microparticles had the best combined mechanical and antioxidant properties as a potential material for food packaging.					
36771173	1	19	dep	waste	286:290	arg1	concerns					292:299	concerns	292:299	concerns	292:299	The development of biobased antioxidant active packaging has been valued by the food industry for complying with environmental and food waste concerns.					
36771173	7	20	theme	highest	1220:1226	arg1	%					1253:1253	~104%	1249:1253	~104%	1249:1253	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	7	20	theme	highest	1220:1226	arg1	elongation					1228:1237	the highest elongation	1216:1237	the highest elongation at break	1216:1246	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	3	21	theme	polyelectrolyte	619:633	arg1	PECs					646:649	PECs	646:649	PECs	646:649	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	3	21	theme	polyelectrolyte	619:633	arg1	complexes					635:643	optimized açaí oil polyelectrolyte complexes	600:643	optimized açaí oil polyelectrolyte complexes (PECs)	600:650	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	6	22	theme	tensile	1027:1033	arg1	strength					1035:1042	The tensile strength	1023:1042	The tensile strength	1023:1042	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	6	23	theme	5	1136:1136	arg1	%					1137:1137	%	1137:1137	%	1137:1137	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	4	24	theme	confocal	686:693	arg1	microscopy					710:719	confocal laser scanning microscopy	686:719	confocal laser scanning microscopy	686:719	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	0	25	theme	Films	65:69	arg1	Properties					36:45	Improved Antioxidant and Mechanical Properties	0:45	Improved Antioxidant and Mechanical Properties of Food Packaging Films	0:69	Improved Antioxidant and Mechanical Properties of Food Packaging Films Based on Chitosan/Deep Eutectic Solvent, Containing Açaí-Filled Microcapsules.					
36771173	3	26	theme	açaí	610:613	arg1	PECs					646:649	PECs	646:649	PECs	646:649	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	3	26	theme	açaí	610:613	arg1	complexes					635:643	optimized açaí oil polyelectrolyte complexes	600:643	optimized açaí oil polyelectrolyte complexes (PECs)	600:650	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	4	27	theme	scanning	701:708	arg1	microscopy					710:719	confocal laser scanning microscopy	686:719	confocal laser scanning microscopy	686:719	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	7	28	contain	containing	1163:1172	arg1	Films					1157:1161	Films	1157:1161	Films containing 2% of PECs	1157:1183	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	7	28	contain	containing	1163:1172	arg2	PECs					1180:1183	PECs	1180:1183	PECs	1180:1183	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	7	28	contain	containing	1163:1172	arg2	%					1175:1175	2%	1174:1175	2% of PECs	1174:1183	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	1	29	theme	antioxidant	178:188	arg1	packaging					197:205	biobased antioxidant active packaging	169:205	biobased antioxidant active packaging	169:205	The development of biobased antioxidant active packaging has been valued by the food industry for complying with environmental and food waste concerns.					
36771173	0	30	theme	Eutectic	94:101	arg1	Solvent					103:109	Chitosan/Deep Eutectic Solvent	80:109	Chitosan/Deep Eutectic Solvent	80:109	Improved Antioxidant and Mechanical Properties of Food Packaging Films Based on Chitosan/Deep Eutectic Solvent, Containing Açaí-Filled Microcapsules.					
36771173	4	31	theme	well-dispersed	794:807	arg1	PECs					809:812	well-dispersed PECs	794:812	well-dispersed PECs	794:812	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	3	32	theme	solution	460:467	arg1	casting					469:475	solution casting	460:475	solution casting	460:475	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	8	33	theme	~17	1366:1368	arg1	mg					1370:1371	mg	1370:1371	mg	1370:1371	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	4	34	theme	composite	748:756	arg1	films					758:762	the chitosan composite films	735:762	the chitosan composite films	735:762	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	4	34	theme	composite	748:756	arg1	continuous					769:778	continuous	769:778	continuous	769:778	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	0	35	theme	Improved	0:7	arg1	Properties					36:45	Improved Antioxidant and Mechanical Properties	0:45	Improved Antioxidant and Mechanical Properties of Food Packaging Films	0:69	Improved Antioxidant and Mechanical Properties of Food Packaging Films Based on Chitosan/Deep Eutectic Solvent, Containing Açaí-Filled Microcapsules.					
36771173	7	36	theme	~6	1207:1208	arg1	%					1209:1209	~6%	1207:1209	~6%	1207:1209	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	8	37	theme	TE	1373:1374	arg1	radicals					1349:1356	DPPH radicals	1344:1356	DPPH radicals	1344:1356	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	8	37	theme	TE	1373:1374	arg1	g-1					1376:1378	~17 mg TE g-1	1366:1378	~17 mg TE g-1	1366:1378	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	5	38	theme	significant	846:856	arg1	influence					858:866	significant influence	846:866	significant influence	846:866	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	10	39	theme	chitosan	1551:1558	arg1	films					1560:1564	the chitosan films	1547:1564	the chitosan films that incorporated 1-2% of microparticles	1547:1605	These results suggested that the chitosan films that incorporated 1-2% of microparticles had the best combined mechanical and antioxidant properties as a potential material for food packaging.					
36771173	3	40	theme	chloride	509:516	arg1	mixture					528:534	a 1:2 choline chloride: glycerol mixture	495:534	a 1:2 choline chloride: glycerol mixture	495:534	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	5	41	theme	increased	819:827	arg1	content					834:840	The increased PECs content	815:840	The increased PECs content	815:840	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	10	42	theme	antioxidant	1644:1654	arg1	properties					1656:1665	the best combined mechanical and antioxidant properties	1611:1665	the best combined mechanical and antioxidant properties as a potential material for food packaging	1611:1708	These results suggested that the chitosan films that incorporated 1-2% of microparticles had the best combined mechanical and antioxidant properties as a potential material for food packaging.					
36771173	7	43	theme	PECs	1180:1183	arg1	%					1175:1175	2%	1174:1175	2% of PECs	1174:1183	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	7	43	theme	PECs	1180:1183	arg1	PECs					1180:1183	PECs	1180:1183	PECs	1180:1183	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	10	44	theme	food	1695:1698	arg1	packaging					1700:1708	food packaging	1695:1708	food packaging	1695:1708	These results suggested that the chitosan films that incorporated 1-2% of microparticles had the best combined mechanical and antioxidant properties as a potential material for food packaging.					
36771173	2	45	theme	chitosan	347:354	arg1	films					366:370	chitosan composite films	347:370	chitosan composite films as a potential active food packaging	347:407	In this work, physicochemical properties for chitosan composite films as a potential active food packaging were investigated.					
36771173	8	46	theme	polyphenols	1418:1428	arg1	g-1					1451:1453	>0.5 mg GAE g-1	1439:1453	>0.5 mg GAE g-1	1439:1453	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	8	46	theme	polyphenols	1418:1428	arg1	content					1430:1436	highest equivalent polyphenols content	1399:1436	highest equivalent polyphenols content (>0.5 mg GAE g-1)	1399:1454	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	8	47	theme	PECs	1273:1276	arg1	%					1268:1268	1%	1267:1268	1% of PECs	1267:1276	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	8	47	theme	PECs	1273:1276	arg1	PECs					1273:1276	PECs	1273:1276	PECs	1273:1276	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	2	48	theme	active	387:392	arg1	packaging					399:407	a potential active food packaging	375:407	a potential active food packaging	375:407	In this work, physicochemical properties for chitosan composite films as a potential active food packaging were investigated.					
36771173	5	49	theme	water	886:890	arg1	permeability					898:909	water vapor permeability	886:909	water vapor permeability	886:909	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	6	50	theme	control	1090:1096	arg1	MPa					1085:1087	11.0 MPa	1080:1087	11.0 MPa (control film)	1080:1102	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	6	50	theme	control	1090:1096	arg1	film					1098:1101	control film	1090:1101	control film	1090:1101	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	3	51	theme	glycerol	519:526	arg1	mixture					528:534	a 1:2 choline chloride: glycerol mixture	495:534	a 1:2 choline chloride: glycerol mixture	495:534	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	1	52	theme	environmental	263:275	arg1	waste					286:290	environmental and food waste	263:290	environmental and food waste concerns	263:299	The development of biobased antioxidant active packaging has been valued by the food industry for complying with environmental and food waste concerns.					
36771173	8	53	theme	mg	1444:1445	arg1	g-1					1451:1453	>0.5 mg GAE g-1	1439:1453	>0.5 mg GAE g-1	1439:1453	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	8	53	theme	mg	1444:1445	arg1	content					1430:1436	highest equivalent polyphenols content	1399:1436	highest equivalent polyphenols content (>0.5 mg GAE g-1)	1399:1454	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	1	54	theme	food	281:284	arg1	waste					286:290	environmental and food waste	263:290	environmental and food waste concerns	263:299	The development of biobased antioxidant active packaging has been valued by the food industry for complying with environmental and food waste concerns.					
36771173	3	55	theme	deep	541:544	arg1	solvent					555:561	a deep eutectic solvent	539:561	a deep eutectic solvent (DES)	539:567	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	3	55	theme	deep	541:544	arg1	films					437:441	Chitosan films	428:441	Chitosan films	428:441	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	3	55	theme	deep	541:544	arg1	DES					564:566	DES	564:566	DES	564:566	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	1	56	theme	active	190:195	arg1	packaging					197:205	biobased antioxidant active packaging	169:205	biobased antioxidant active packaging	169:205	The development of biobased antioxidant active packaging has been valued by the food industry for complying with environmental and food waste concerns.					
36771173	0	57	theme	Food	50:53	arg1	Films					65:69	Food Packaging Films	50:69	Food Packaging Films	50:69	Improved Antioxidant and Mechanical Properties of Food Packaging Films Based on Chitosan/Deep Eutectic Solvent, Containing Açaí-Filled Microcapsules.					
36771173	5	58	theme	vapor	892:896	arg1	permeability					898:909	water vapor permeability	886:909	water vapor permeability	886:909	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	6	59	theme	%	1117:1117	arg1	DES					1119:1121	5% DES	1116:1121	5% DES	1116:1121	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	6	59	theme	%	1117:1117	arg1	MPa					1111:1113	0.74 MPa	1106:1113	0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs)	1106:1154	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	4	60	theme	electron	662:669	arg1	microscopy					671:680	Scanning electron microscopy	653:680	Scanning electron microscopy	653:680	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	8	61	with	Films	1256:1260	arg1	%					1268:1268	1%	1267:1268	1% of PECs	1267:1276	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	8	61	with	Films	1256:1260	arg1	PECs					1273:1276	PECs	1273:1276	PECs	1273:1276	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	7	62	contain	had	1185:1187	arg1	Films					1157:1161	Films	1157:1161	Films containing 2% of PECs	1157:1183	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	7	62	contain	had	1185:1187	arg2	CrD					1202:1204	an increased CrD	1189:1204	an increased CrD	1189:1204	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	7	62	contain	had	1185:1187	arg2	%					1209:1209	~6%	1207:1209	~6%	1207:1209	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	7	62	contain	had	1185:1187	arg2	elongation					1228:1237	the highest elongation	1216:1237	the highest elongation at break	1216:1246	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	7	62	contain	had	1185:1187	arg2	%					1253:1253	~104%	1249:1253	~104%	1249:1253	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	8	63	theme	antioxidant	1300:1310	arg1	properties					1312:1321	the highest antioxidant properties	1288:1321	the highest antioxidant properties against the ABTS	1288:1338	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	6	64	theme	%	1148:1148	arg1	PECs					1150:1153	5% PECs	1147:1153	5% PECs	1147:1153	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	3	65	theme	oil	615:617	arg1	PECs					646:649	PECs	646:649	PECs	646:649	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	3	65	theme	oil	615:617	arg1	complexes					635:643	optimized açaí oil polyelectrolyte complexes	600:643	optimized açaí oil polyelectrolyte complexes (PECs)	600:650	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	5	66	theme	mechanical	936:945	arg1	behavior					959:966	mechanical and dynamic behavior	936:966	behavior	959:966	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	6	67	theme	%	1137:1137	arg1	DES					1139:1141	5% DES	1136:1141	5% DES	1136:1141	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	3	68	theme	1:2	497:499	arg1	mixture					528:534	a 1:2 choline chloride: glycerol mixture	495:534	a 1:2 choline chloride: glycerol mixture	495:534	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	3	69	theme	optimized	600:608	arg1	PECs					646:649	PECs	646:649	PECs	646:649	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	3	69	theme	optimized	600:608	arg1	complexes					635:643	optimized açaí oil polyelectrolyte complexes	600:643	optimized açaí oil polyelectrolyte complexes (PECs)	600:650	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	8	70	theme	DPPH	1344:1347	arg1	radicals					1349:1356	DPPH radicals	1344:1356	DPPH radicals	1344:1356	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	8	70	theme	DPPH	1344:1347	arg1	g-1					1376:1378	~17 mg TE g-1	1366:1378	~17 mg TE g-1	1366:1378	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	8	70	theme	DPPH	1344:1347	arg1	~6					1359:1360	~6	1359:1360	~6	1359:1360	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	0	71	theme	Chitosan/Deep	80:92	arg1	Solvent					103:109	Chitosan/Deep Eutectic Solvent	80:109	Chitosan/Deep Eutectic Solvent	80:109	Improved Antioxidant and Mechanical Properties of Food Packaging Films Based on Chitosan/Deep Eutectic Solvent, Containing Açaí-Filled Microcapsules.					
36771173	1	72	theme	biobased	169:176	arg1	packaging					197:205	biobased antioxidant active packaging	169:205	biobased antioxidant active packaging	169:205	The development of biobased antioxidant active packaging has been valued by the food industry for complying with environmental and food waste concerns.					
36771173	4	73	theme	laser	695:699	arg1	microscopy					710:719	confocal laser scanning microscopy	686:719	confocal laser scanning microscopy	686:719	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	5	74	theme	films	975:979	arg1	properties					1011:1020	their antioxidant properties	993:1020	their antioxidant properties	993:1020	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	5	74	theme	films	975:979	arg1	thickness					875:883	the thickness	871:883	the thickness	871:883	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	5	74	theme	films	975:979	arg1	crystallinity					912:924	crystallinity	912:924	crystallinity (CrD)	912:930	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	5	74	theme	films	975:979	arg1	CrD					927:929	CrD	927:929	CrD	927:929	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	5	74	theme	films	975:979	arg1	behavior					959:966	mechanical and dynamic behavior	936:966	behavior	959:966	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	5	74	theme	films	975:979	arg1	permeability					898:909	water vapor permeability	886:909	water vapor permeability	886:909	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	10	75	theme	mechanical	1629:1638	arg1	properties					1656:1665	the best combined mechanical and antioxidant properties	1611:1665	the best combined mechanical and antioxidant properties as a potential material for food packaging	1611:1708	These results suggested that the chitosan films that incorporated 1-2% of microparticles had the best combined mechanical and antioxidant properties as a potential material for food packaging.					
36771173	8	76	theme	mg	1370:1371	arg1	radicals					1349:1356	DPPH radicals	1344:1356	DPPH radicals	1344:1356	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	8	76	theme	mg	1370:1371	arg1	g-1					1376:1378	~17 mg TE g-1	1366:1378	~17 mg TE g-1	1366:1378	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	5	77	theme	PECs	829:832	arg1	content					834:840	The increased PECs content	815:840	The increased PECs content	815:840	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	5	78	theme	dynamic	951:957	arg1	behavior					959:966	mechanical and dynamic behavior	936:966	behavior	959:966	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	10	79	contain	had	1607:1609	arg1	films					1560:1564	the chitosan films	1547:1564	the chitosan films that incorporated 1-2% of microparticles	1547:1605	These results suggested that the chitosan films that incorporated 1-2% of microparticles had the best combined mechanical and antioxidant properties as a potential material for food packaging.					
36771173	10	79	contain	had	1607:1609	arg2	properties					1656:1665	the best combined mechanical and antioxidant properties	1611:1665	the best combined mechanical and antioxidant properties as a potential material for food packaging	1611:1708	These results suggested that the chitosan films that incorporated 1-2% of microparticles had the best combined mechanical and antioxidant properties as a potential material for food packaging.					
36771173	4	80	theme	chitosan	739:746	arg1	films					758:762	the chitosan composite films	735:762	the chitosan composite films	735:762	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	4	80	theme	chitosan	739:746	arg1	continuous					769:778	continuous	769:778	continuous	769:778	Scanning electron microscopy and confocal laser scanning microscopy revealed that the chitosan composite films were continuous and contained well-dispersed PECs.					
36771173	2	81	theme	food	394:397	arg1	packaging					399:407	a potential active food packaging	375:407	a potential active food packaging	375:407	In this work, physicochemical properties for chitosan composite films as a potential active food packaging were investigated.					
36771173	0	82	theme	Mechanical	25:34	arg1	Properties					36:45	Improved Antioxidant and Mechanical Properties	0:45	Improved Antioxidant and Mechanical Properties of Food Packaging Films	0:69	Improved Antioxidant and Mechanical Properties of Food Packaging Films Based on Chitosan/Deep Eutectic Solvent, Containing Açaí-Filled Microcapsules.					
36771173	6	83	dep	MPa	1111:1113	arg1	MPa					1131:1133	> 0.63 MPa	1124:1133	0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs)	1106:1154	The tensile strength was reduced in the following order: 11.0 MPa (control film) > 0.74 MPa (5% DES) > 0.63 MPa (5% DES and 5% PECs).					
36771173	7	84	from	break	1242:1246	arg1	%					1253:1253	~104%	1249:1253	~104%	1249:1253	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	7	84	from	break	1242:1246	arg1	CrD					1202:1204	an increased CrD	1189:1204	an increased CrD	1189:1204	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	7	84	from	break	1242:1246	arg1	%					1209:1209	~6%	1207:1209	~6%	1207:1209	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	7	84	from	break	1242:1246	arg1	elongation					1228:1237	the highest elongation	1216:1237	the highest elongation at break	1216:1246	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	7	85	theme	increased	1192:1200	arg1	CrD					1202:1204	an increased CrD	1189:1204	an increased CrD	1189:1204	Films containing 2% of PECs had an increased CrD, ~6%, and the highest elongation at break, ~104%.					
36771173	10	86	theme	potential	1672:1680	arg1	material					1682:1689	a potential material	1670:1689	a potential material for food packaging	1670:1708	These results suggested that the chitosan films that incorporated 1-2% of microparticles had the best combined mechanical and antioxidant properties as a potential material for food packaging.					
36771173	3	87	theme	complexes	635:643	arg1	%					595:595	0-10%	591:595	0-10% of optimized açaí oil polyelectrolyte complexes (PECs)	591:650	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	3	87	theme	complexes	635:643	arg1	PECs					646:649	PECs	646:649	PECs	646:649	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	3	87	theme	complexes	635:643	arg1	complexes					635:643	optimized açaí oil polyelectrolyte complexes	600:643	optimized açaí oil polyelectrolyte complexes (PECs)	600:650	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	0	88	theme	Packaging	55:63	arg1	Films					65:69	Food Packaging Films	50:69	Food Packaging Films	50:69	Improved Antioxidant and Mechanical Properties of Food Packaging Films Based on Chitosan/Deep Eutectic Solvent, Containing Açaí-Filled Microcapsules.					
36771173	3	89	theme	Chitosan	428:435	arg1	solvent					555:561	a deep eutectic solvent	539:561	a deep eutectic solvent (DES)	539:567	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	3	89	theme	Chitosan	428:435	arg1	films					437:441	Chitosan films	428:441	Chitosan films	428:441	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	8	90	theme	equivalent	1407:1416	arg1	g-1					1451:1453	>0.5 mg GAE g-1	1439:1453	>0.5 mg GAE g-1	1439:1453	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	8	90	theme	equivalent	1407:1416	arg1	content					1430:1436	highest equivalent polyphenols content	1399:1436	highest equivalent polyphenols content (>0.5 mg GAE g-1)	1399:1454	Films with 1% of PECs displayed the highest antioxidant properties against the ABTS and DPPH radicals, ~6 and ~17 mg TE g-1, respectively, and highest equivalent polyphenols content (>0.5 mg GAE g-1).					
36771173	2	91	theme	composite	356:364	arg1	films					366:370	chitosan composite films	347:370	chitosan composite films as a potential active food packaging	347:407	In this work, physicochemical properties for chitosan composite films as a potential active food packaging were investigated.					
36771173	3	92	theme	choline	501:507	arg1	mixture					528:534	a 1:2 choline chloride: glycerol mixture	495:534	a 1:2 choline chloride: glycerol mixture	495:534	Chitosan films were prepared by solution casting, plasticized with a 1:2 choline chloride: glycerol mixture as a deep eutectic solvent (DES) and incorporated with 0-10% of optimized açaí oil polyelectrolyte complexes (PECs).					
36771173	5	93	theme	antioxidant	999:1009	arg1	properties					1011:1020	their antioxidant properties	993:1020	their antioxidant properties	993:1020	The increased PECs content had significant influence on the thickness, water vapor permeability, crystallinity (CrD) and mechanical and dynamic behavior of the films, as well as their antioxidant properties.					
36771173	0	94	theme	Antioxidant	9:19	arg1	Properties					36:45	Improved Antioxidant and Mechanical Properties	0:45	Improved Antioxidant and Mechanical Properties of Food Packaging Films	0:69	Improved Antioxidant and Mechanical Properties of Food Packaging Films Based on Chitosan/Deep Eutectic Solvent, Containing Açaí-Filled Microcapsules.					
37100323	0	0	theme	infected	100:107	arg1	healing					115:121	infected wound healing	100:121	infected wound healing	100:121	Interfacial assembly of chitin/Mn3O4 composite hydrogels as photothermal antibacterial platform for infected wound healing.					
37100323	5	1	dep	the	817:819	arg1	assist					821:826	assist	821:826	assist of NIR	821:833	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	6	2	theme	wound	1185:1189	arg1	therapy					1201:1207	the bacterial-associated wound infection therapy	1160:1207	the bacterial-associated wound infection therapy	1160:1207	This study broadens the scope for the fabrication of chitin hydrogels with antibacterial property, and offers an excellent alternative for the bacterial-associated wound infection therapy.					
37100323	6	3	theme	infection	1191:1199	arg1	therapy					1201:1207	the bacterial-associated wound infection therapy	1160:1207	the bacterial-associated wound infection therapy	1160:1207	This study broadens the scope for the fabrication of chitin hydrogels with antibacterial property, and offers an excellent alternative for the bacterial-associated wound infection therapy.					
37100323	2	4	theme	healing	392:398	arg1	application					400:410	infected wound healing application	377:410	infected wound healing application	377:410	Herein, this study developed a series of bioactive chitin/Mn3O4 composite hydrogels under mild conditions for infected wound healing application.					
37100323	2	5	theme	chitin/Mn3O4	318:329	arg1	hydrogels					341:349	bioactive chitin/Mn3O4 composite hydrogels	308:349	bioactive chitin/Mn3O4 composite hydrogels	308:349	Herein, this study developed a series of bioactive chitin/Mn3O4 composite hydrogels under mild conditions for infected wound healing application.					
37100323	6	6	theme	bacterial-associated	1164:1183	arg1	therapy					1201:1207	the bacterial-associated wound infection therapy	1160:1207	the bacterial-associated wound infection therapy	1160:1207	This study broadens the scope for the fabrication of chitin hydrogels with antibacterial property, and offers an excellent alternative for the bacterial-associated wound infection therapy.					
37100323	0	7	theme	wound	109:113	arg1	healing					115:121	infected wound healing	100:121	infected wound healing	100:121	Interfacial assembly of chitin/Mn3O4 composite hydrogels as photothermal antibacterial platform for infected wound healing.					
37100323	2	8	theme	bioactive	308:316	arg1	hydrogels					341:349	bioactive chitin/Mn3O4 composite hydrogels	308:349	bioactive chitin/Mn3O4 composite hydrogels	308:349	Herein, this study developed a series of bioactive chitin/Mn3O4 composite hydrogels under mild conditions for infected wound healing application.					
37100323	5	9	theme	phase	971:975	arg1	transition					977:986	the phase transition	967:986	the phase transition from inflammation to remodeling	967:1018	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	5	10	theme	excellent	843:851	arg1	healing					864:870	an excellent skin wound healing	840:870	an excellent skin wound healing performance	840:882	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	5	11	theme	aureus	913:918	arg1	model					944:948	a mouse full-thickness S. aureus biofilms-infected wound model	887:948	a mouse full-thickness S. aureus biofilms-infected wound model	887:948	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	3	12	theme	chitin	483:488	arg1	networks					490:497	chitin networks	483:497	chitin networks	483:497	The in situ synthesized Mn3O4 NPs homogeneously distribute throughout chitin networks and strongly interact with chitin matrix, and as well as endow the chitin/Mn3O4 hydrogels with NIR-assisted outstanding photothermal antibacterial and antibiofilm activities.					
37100323	1	13	theme	alternative	183:193	arg1	dressings					215:223	alternative antibacterial wound dressings	183:223	alternative antibacterial wound dressings independent of antibiotics	183:250	To combat bacteria and even biofilm infections, developing alternative antibacterial wound dressings independent of antibiotics is imperative.					
37100323	5	14	theme	skin	853:856	arg1	healing					864:870	an excellent skin wound healing	840:870	an excellent skin wound healing performance	840:882	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	3	15	theme	Mn3O4	437:441	arg1	NPs					443:445	The in situ synthesized Mn3O4 NPs	413:445	The in situ synthesized Mn3O4 NPs	413:445	The in situ synthesized Mn3O4 NPs homogeneously distribute throughout chitin networks and strongly interact with chitin matrix, and as well as endow the chitin/Mn3O4 hydrogels with NIR-assisted outstanding photothermal antibacterial and antibiofilm activities.					
37100323	1	16	theme	antibacterial	195:207	arg1	dressings					215:223	alternative antibacterial wound dressings	183:223	alternative antibacterial wound dressings independent of antibiotics	183:250	To combat bacteria and even biofilm infections, developing alternative antibacterial wound dressings independent of antibiotics is imperative.					
37100323	3	17	theme	synthesized	425:435	arg1	NPs					443:445	The in situ synthesized Mn3O4 NPs	413:445	The in situ synthesized Mn3O4 NPs	413:445	The in situ synthesized Mn3O4 NPs homogeneously distribute throughout chitin networks and strongly interact with chitin matrix, and as well as endow the chitin/Mn3O4 hydrogels with NIR-assisted outstanding photothermal antibacterial and antibiofilm activities.					
37100323	2	18	theme	mild	357:360	arg1	conditions					362:371	mild conditions	357:371	mild conditions for infected wound healing application	357:410	Herein, this study developed a series of bioactive chitin/Mn3O4 composite hydrogels under mild conditions for infected wound healing application.					
37100323	1	19	theme	wound	209:213	arg1	dressings					215:223	alternative antibacterial wound dressings	183:223	alternative antibacterial wound dressings independent of antibiotics	183:250	To combat bacteria and even biofilm infections, developing alternative antibacterial wound dressings independent of antibiotics is imperative.					
37100323	0	20	theme	Interfacial	0:10	arg1	assembly					12:19	Interfacial assembly	0:19	Interfacial assembly of chitin/Mn3O4 composite	0:45	Interfacial assembly of chitin/Mn3O4 composite hydrogels as photothermal antibacterial platform for infected wound healing.					
37100323	6	21	theme	antibacterial	1096:1108	arg1	property					1110:1117	antibacterial property	1096:1117	antibacterial property	1096:1117	This study broadens the scope for the fabrication of chitin hydrogels with antibacterial property, and offers an excellent alternative for the bacterial-associated wound infection therapy.					
37100323	3	22	theme	antibiofilm	650:660	arg1	activities					662:671	NIR-assisted outstanding photothermal antibacterial and antibiofilm activities	594:671	activities	662:671	The in situ synthesized Mn3O4 NPs homogeneously distribute throughout chitin networks and strongly interact with chitin matrix, and as well as endow the chitin/Mn3O4 hydrogels with NIR-assisted outstanding photothermal antibacterial and antibiofilm activities.					
37100323	0	23	theme	chitin/Mn3O4	24:35	arg1	composite					37:45	chitin/Mn3O4 composite	24:45	chitin/Mn3O4 composite	24:45	Interfacial assembly of chitin/Mn3O4 composite hydrogels as photothermal antibacterial platform for infected wound healing.					
37100323	5	24	theme	biofilms-infected	920:936	arg1	model					944:948	a mouse full-thickness S. aureus biofilms-infected wound model	887:948	a mouse full-thickness S. aureus biofilms-infected wound model	887:948	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	2	25	theme	hydrogels	341:349	arg1	series					298:303	a series	296:303	a series of bioactive chitin/Mn3O4 composite hydrogels	296:349	Herein, this study developed a series of bioactive chitin/Mn3O4 composite hydrogels under mild conditions for infected wound healing application.					
37100323	1	26	theme	independent	225:235	arg1	dressings					215:223	alternative antibacterial wound dressings	183:223	alternative antibacterial wound dressings independent of antibiotics	183:250	To combat bacteria and even biofilm infections, developing alternative antibacterial wound dressings independent of antibiotics is imperative.					
37100323	5	27	from	inflammation	993:1004	arg1	transition					977:986	the phase transition	967:986	the phase transition from inflammation to remodeling	967:1018	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	6	28	theme	hydrogels	1081:1089	arg1	fabrication					1059:1069	the fabrication	1055:1069	the fabrication of chitin hydrogels with antibacterial property	1055:1117	This study broadens the scope for the fabrication of chitin hydrogels with antibacterial property, and offers an excellent alternative for the bacterial-associated wound infection therapy.					
37100323	2	29	theme	composite	331:339	arg1	hydrogels					341:349	bioactive chitin/Mn3O4 composite hydrogels	308:349	bioactive chitin/Mn3O4 composite hydrogels	308:349	Herein, this study developed a series of bioactive chitin/Mn3O4 composite hydrogels under mild conditions for infected wound healing application.					
37100323	4	30	theme	favorable	719:727	arg1	biocompatibility					729:744	favorable biocompatibility	719:744	favorable biocompatibility	719:744	Meantime, the chitin/Mn3O4 hydrogels exhibit favorable biocompatibility and antioxidant property.					
37100323	3	31	with	hydrogels	579:587	arg1	antibacterial					632:644	antibacterial	632:644	antibacterial	632:644	The in situ synthesized Mn3O4 NPs homogeneously distribute throughout chitin networks and strongly interact with chitin matrix, and as well as endow the chitin/Mn3O4 hydrogels with NIR-assisted outstanding photothermal antibacterial and antibiofilm activities.					
37100323	3	31	with	hydrogels	579:587	arg1	activities					662:671	NIR-assisted outstanding photothermal antibacterial and antibiofilm activities	594:671	activities	662:671	The in situ synthesized Mn3O4 NPs homogeneously distribute throughout chitin networks and strongly interact with chitin matrix, and as well as endow the chitin/Mn3O4 hydrogels with NIR-assisted outstanding photothermal antibacterial and antibiofilm activities.					
37100323	6	32	theme	chitin	1074:1079	arg1	hydrogels					1081:1089	chitin hydrogels	1074:1089	chitin hydrogels with antibacterial property	1074:1117	This study broadens the scope for the fabrication of chitin hydrogels with antibacterial property, and offers an excellent alternative for the bacterial-associated wound infection therapy.					
37100323	4	33	theme	antioxidant	750:760	arg1	property					762:769	antioxidant property	750:769	antioxidant property	750:769	Meantime, the chitin/Mn3O4 hydrogels exhibit favorable biocompatibility and antioxidant property.					
37100323	1	34	theme	antibiotics	240:250	arg1	independent					225:235	independent	225:235	independent	225:235	To combat bacteria and even biofilm infections, developing alternative antibacterial wound dressings independent of antibiotics is imperative.					
37100323	0	35	theme	composite	37:45	arg1	assembly					12:19	Interfacial assembly	0:19	Interfacial assembly of chitin/Mn3O4 composite	0:45	Interfacial assembly of chitin/Mn3O4 composite hydrogels as photothermal antibacterial platform for infected wound healing.					
37100323	5	36	theme	mouse	889:893	arg1	model					944:948	a mouse full-thickness S. aureus biofilms-infected wound model	887:948	a mouse full-thickness S. aureus biofilms-infected wound model	887:948	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	5	37	theme	wound	938:942	arg1	model					944:948	a mouse full-thickness S. aureus biofilms-infected wound model	887:948	a mouse full-thickness S. aureus biofilms-infected wound model	887:948	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	0	38	theme	photothermal	60:71	arg1	platform					87:94	photothermal antibacterial platform	60:94	photothermal antibacterial platform for infected wound healing	60:121	Interfacial assembly of chitin/Mn3O4 composite hydrogels as photothermal antibacterial platform for infected wound healing.					
37100323	5	39	theme	healing	864:870	arg1	performance					872:882	an excellent skin wound healing performance	840:882	an excellent skin wound healing performance	840:882	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	2	40	theme	wound	386:390	arg1	healing					392:398	infected wound healing	377:398	infected wound healing application	377:410	Herein, this study developed a series of bioactive chitin/Mn3O4 composite hydrogels under mild conditions for infected wound healing application.					
37100323	5	41	theme	chitin/Mn3O4	789:800	arg1	hydrogels					802:810	the chitin/Mn3O4 hydrogels	785:810	the chitin/Mn3O4 hydrogels with the assist of NIR	785:833	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	5	42	with	hydrogels	802:810	arg1	the					817:819	the	817:819	the	817:819	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	2	43	theme	infected	377:384	arg1	healing					392:398	infected wound healing	377:398	infected wound healing application	377:410	Herein, this study developed a series of bioactive chitin/Mn3O4 composite hydrogels under mild conditions for infected wound healing application.					
37100323	5	44	theme	full-thickness	895:908	arg1	model					944:948	a mouse full-thickness S. aureus biofilms-infected wound model	887:948	a mouse full-thickness S. aureus biofilms-infected wound model	887:948	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	3	45	dep	well	548:551	arg1	as					553:554	as	553:554	as	553:554	The in situ synthesized Mn3O4 NPs homogeneously distribute throughout chitin networks and strongly interact with chitin matrix, and as well as endow the chitin/Mn3O4 hydrogels with NIR-assisted outstanding photothermal antibacterial and antibiofilm activities.					
37100323	6	46	with	hydrogels	1081:1089	arg1	property					1110:1117	antibacterial property	1096:1117	antibacterial property	1096:1117	This study broadens the scope for the fabrication of chitin hydrogels with antibacterial property, and offers an excellent alternative for the bacterial-associated wound infection therapy.					
37100323	1	47	theme	even	147:150	arg1	infections					160:169	even biofilm infections	147:169	even biofilm infections	147:169	To combat bacteria and even biofilm infections, developing alternative antibacterial wound dressings independent of antibiotics is imperative.					
37100323	5	48	theme	wound	858:862	arg1	healing					864:870	an excellent skin wound healing	840:870	an excellent skin wound healing performance	840:882	Furthermore, the chitin/Mn3O4 hydrogels with the assist of NIR show an excellent skin wound healing performance in a mouse full-thickness S. aureus biofilms-infected wound model, by accelerating the phase transition from inflammation to remodeling.					
37100323	3	49	theme	chitin	526:531	arg1	matrix					533:538	chitin matrix	526:538	chitin matrix	526:538	The in situ synthesized Mn3O4 NPs homogeneously distribute throughout chitin networks and strongly interact with chitin matrix, and as well as endow the chitin/Mn3O4 hydrogels with NIR-assisted outstanding photothermal antibacterial and antibiofilm activities.					
37100323	1	50	theme	biofilm	152:158	arg1	infections					160:169	even biofilm infections	147:169	even biofilm infections	147:169	To combat bacteria and even biofilm infections, developing alternative antibacterial wound dressings independent of antibiotics is imperative.					
37100323	6	51	theme	excellent	1134:1142	arg1	alternative					1144:1154	an excellent alternative	1131:1154	an excellent alternative for the bacterial-associated wound infection therapy	1131:1207	This study broadens the scope for the fabrication of chitin hydrogels with antibacterial property, and offers an excellent alternative for the bacterial-associated wound infection therapy.					
37100323	0	52	theme	antibacterial	73:85	arg1	platform					87:94	photothermal antibacterial platform	60:94	photothermal antibacterial platform for infected wound healing	60:121	Interfacial assembly of chitin/Mn3O4 composite hydrogels as photothermal antibacterial platform for infected wound healing.					
37100323	3	53	theme	chitin/Mn3O4	566:577	arg1	hydrogels					579:587	the chitin/Mn3O4 hydrogels	562:587	the chitin/Mn3O4 hydrogels with NIR-assisted outstanding photothermal antibacterial and antibiofilm activities	562:671	The in situ synthesized Mn3O4 NPs homogeneously distribute throughout chitin networks and strongly interact with chitin matrix, and as well as endow the chitin/Mn3O4 hydrogels with NIR-assisted outstanding photothermal antibacterial and antibiofilm activities.					
37003391	2	0	theme	polyethyleneimine	476:492	arg1	composite					525:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	In this paper, Zr4+ and glutaraldehyde crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite was successfully synthesized and characterized by XRD, SEM, FTIR, BET, and XPS.					
37003391	1	1	theme	effective	306:314	arg1	strategy					316:323	an effective strategy	303:323	an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking	303:422	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	1	theme	effective	306:314	arg1	interaction					227:237	electrostatic interaction	213:237	electrostatic interaction	213:237	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	1	theme	effective	306:314	arg1	it					297:298	it	297:298	it	297:298	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	1	theme	effective	306:314	arg1	ability					256:262	ion exchange ability	243:262	ion exchange ability	243:262	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	1	theme	effective	306:314	arg1	removal					288:294	removal	288:294	removal	288:294	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	2	2	dep	Zr4+	440:443	arg1	composite					525:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	In this paper, Zr4+ and glutaraldehyde crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite was successfully synthesized and characterized by XRD, SEM, FTIR, BET, and XPS.					
37003391	9	3	theme	ion	1623:1625	arg1	-NH2/-C=N					1589:1597	-NH2/-C=N	1589:1597	-NH2/-C=N	1589:1597	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	9	3	theme	ion	1623:1625	arg1	exchange					1607:1614	ion exchange	1603:1614	ion exchange	1603:1614	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	6	4	theme	adsorption	1034:1043	arg1	process					1045:1051	the adsorption process	1030:1051	the adsorption process of Cr (VI) by CGPZ	1030:1070	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	6	4	theme	adsorption	1034:1043	arg1	process					1101:1107	a spontaneous endothermic process	1075:1107	a spontaneous endothermic process	1075:1107	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	8	5	theme	Cr	1427:1428	arg1	removal					1416:1422	The removal	1412:1422	The removal of Cr(VI)	1412:1432	The removal of Cr(VI) mainly involves electrostatic attraction, ion exchange, reduction and complexation.					
37003391	2	6	theme	crosslinked	464:474	arg1	composite					525:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	In this paper, Zr4+ and glutaraldehyde crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite was successfully synthesized and characterized by XRD, SEM, FTIR, BET, and XPS.					
37003391	7	7	theme	acid-base	1361:1369	arg1	resistance					1371:1380	good acid-base resistance	1356:1380	good acid-base resistance	1356:1380	The regeneration experiments show that both HCl and NaOH can effectively desorb Cr(III) and Cr(VI) from the adsorbent surface, and the adsorbent has good acid-base resistance and regeneration performance.					
37003391	9	8	theme	electrostatic	1560:1572	arg1	interaction					1574:1584	electrostatic interaction	1560:1584	electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion	1560:1625	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	0	9	link	cross-linked	24:35	arg1	composite					79:87	glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite	9:87	glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite	9:87	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	7	10	contain	has	1352:1354	arg2	performance					1399:1409	regeneration performance	1386:1409	regeneration performance	1386:1409	The regeneration experiments show that both HCl and NaOH can effectively desorb Cr(III) and Cr(VI) from the adsorbent surface, and the adsorbent has good acid-base resistance and regeneration performance.					
37003391	7	10	contain	has	1352:1354	arg2	resistance					1371:1380	good acid-base resistance	1356:1380	good acid-base resistance	1356:1380	The regeneration experiments show that both HCl and NaOH can effectively desorb Cr(III) and Cr(VI) from the adsorbent surface, and the adsorbent has good acid-base resistance and regeneration performance.					
37003391	7	10	contain	has	1352:1354	arg1	adsorbent					1342:1350	adsorbent	1342:1350	adsorbent	1342:1350	The regeneration experiments show that both HCl and NaOH can effectively desorb Cr(III) and Cr(VI) from the adsorbent surface, and the adsorbent has good acid-base resistance and regeneration performance.					
37003391	4	11	theme	Cr	853:854	arg1	capacity					841:848	The monolayer maximum adsorption capacity	808:848	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ	808:866	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	4	11	theme	Cr	853:854	arg1	593.72 mg g-1					872:884	593.72 mg g-1	872:884	593.72 mg g-1	872:884	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	0	12	theme	Cr	119:120	arg1	performance					137:147	Cr(VI) adsorption performance	119:147	Cr(VI) adsorption performance	119:147	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	0	12	theme	Cr	119:120	arg1	Zr4+					0:3	Zr4+	0:3	Zr4+	0:3	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	9	13	dep	Cr	1550:1551	arg1	VI					1553:1554	VI	1553:1554	VI	1553:1554	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	9	14	from	pH = 2.0	1689:1696	arg1	%					1684:1684	45.4 %	1679:1684	45.4 %	1679:1684	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	1	15	theme	chitosan	267:274	arg1	strategy					316:323	an effective strategy	303:323	an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking	303:422	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	15	theme	chitosan	267:274	arg1	interaction					227:237	electrostatic interaction	213:237	electrostatic interaction	213:237	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	15	theme	chitosan	267:274	arg1	it					297:298	it	297:298	it	297:298	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	15	theme	chitosan	267:274	arg1	removal					288:294	removal	288:294	removal	288:294	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	15	theme	chitosan	267:274	arg1	ability					256:262	ion exchange ability	243:262	ion exchange ability	243:262	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	3	16	dep	ZrO	745:747	arg1	bonds					757:761	bonds	757:761	bonds	757:761	The results showed that polyethyleneimine was successfully grafted onto chitosan by Schiff base reaction, while the appearance of ZrO and ZrN bonds verified the successful preparation of CGPZ.					
37003391	3	17	theme	CGPZ	802:805	arg1	preparation					787:797	the successful preparation	772:797	the successful preparation of CGPZ	772:805	The results showed that polyethyleneimine was successfully grafted onto chitosan by Schiff base reaction, while the appearance of ZrO and ZrN bonds verified the successful preparation of CGPZ.					
37003391	2	18	theme	CGPZ	519:522	arg1	composite					525:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	In this paper, Zr4+ and glutaraldehyde crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite was successfully synthesized and characterized by XRD, SEM, FTIR, BET, and XPS.					
37003391	1	19	theme	polyvalent	338:347	arg1	ions					355:358	polyvalent metal ions	338:358	polyvalent metal ions	338:358	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	4	20	theme	maximum	822:828	arg1	capacity					841:848	The monolayer maximum adsorption capacity	808:848	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ	808:866	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	4	20	theme	maximum	822:828	arg1	593.72 mg g-1					872:884	593.72 mg g-1	872:884	593.72 mg g-1	872:884	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	6	21	theme	isotherm	991:998	arg1	results					1012:1018	The thermodynamic, isotherm and kinetic results	972:1018	The thermodynamic, isotherm and kinetic results	972:1018	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	5	22	theme	100 mg L-1	938:947	arg1	Cr					949:950	100 mg L-1 Cr	938:950	100 mg L-1 Cr	938:950	The removal efficiency of 100 mg L-1 Cr(VI) reached 95.7 %.					
37003391	9	23	theme	-OH	1706:1708	arg1	group					1710:1714	the -OH group	1702:1714	the -OH group on its surface	1702:1729	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	4	24	from	t = 210 min	899:909	arg1	capacity					841:848	The monolayer maximum adsorption capacity	808:848	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ	808:866	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	4	24	from	t = 210 min	899:909	arg1	593.72 mg g-1					872:884	593.72 mg g-1	872:884	593.72 mg g-1	872:884	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	1	25	theme	metal	349:353	arg1	ions					355:358	polyvalent metal ions	338:358	polyvalent metal ions	338:358	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	4	26	theme	monolayer	812:820	arg1	capacity					841:848	The monolayer maximum adsorption capacity	808:848	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ	808:866	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	4	26	theme	monolayer	812:820	arg1	593.72 mg g-1					872:884	593.72 mg g-1	872:884	593.72 mg g-1	872:884	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	5	27	theme	Cr	949:950	arg1	efficiency					924:933	The removal efficiency	912:933	The removal efficiency of 100 mg L-1 Cr	912:950	The removal efficiency of 100 mg L-1 Cr(VI) reached 95.7 %.					
37003391	3	28	theme	Schiff	699:704	arg1	reaction					711:718	Schiff base reaction	699:718	Schiff base reaction	699:718	The results showed that polyethyleneimine was successfully grafted onto chitosan by Schiff base reaction, while the appearance of ZrO and ZrN bonds verified the successful preparation of CGPZ.					
37003391	3	29	theme	base	706:709	arg1	reaction					711:718	Schiff base reaction	699:718	Schiff base reaction	699:718	The results showed that polyethyleneimine was successfully grafted onto chitosan by Schiff base reaction, while the appearance of ZrO and ZrN bonds verified the successful preparation of CGPZ.					
37003391	6	30	theme	pseudo-second-order	1172:1190	arg1	model					1200:1204	pseudo-second-order kinetic model	1172:1204	pseudo-second-order kinetic model	1172:1204	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	8	31	dep	Cr	1427:1428	arg1	VI					1430:1431	VI	1430:1431	VI	1430:1431	The removal of Cr(VI) mainly involves electrostatic attraction, ion exchange, reduction and complexation.					
37003391	7	32	theme	good	1356:1359	arg1	resistance					1371:1380	good acid-base resistance	1356:1380	good acid-base resistance	1356:1380	The regeneration experiments show that both HCl and NaOH can effectively desorb Cr(III) and Cr(VI) from the adsorbent surface, and the adsorbent has good acid-base resistance and regeneration performance.					
37003391	7	33	dep	Cr	1299:1300	arg1	VI					1302:1303	VI	1302:1303	VI	1302:1303	The regeneration experiments show that both HCl and NaOH can effectively desorb Cr(III) and Cr(VI) from the adsorbent surface, and the adsorbent has good acid-base resistance and regeneration performance.					
37003391	1	34	dep	strategy	316:323	arg1	VI					284:285	VI	284:285	VI	284:285	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	4	35	theme	adsorption	830:839	arg1	capacity					841:848	The monolayer maximum adsorption capacity	808:848	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ	808:866	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	4	35	theme	adsorption	830:839	arg1	593.72 mg g-1					872:884	593.72 mg g-1	872:884	593.72 mg g-1	872:884	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	7	36	theme	regeneration	1386:1397	arg1	performance					1399:1409	regeneration performance	1386:1409	regeneration performance	1386:1409	The regeneration experiments show that both HCl and NaOH can effectively desorb Cr(III) and Cr(VI) from the adsorbent surface, and the adsorbent has good acid-base resistance and regeneration performance.					
37003391	6	37	theme	kinetic	1004:1010	arg1	results					1012:1018	The thermodynamic, isotherm and kinetic results	972:1018	The thermodynamic, isotherm and kinetic results	972:1018	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	0	38	theme	cross-linked	24:35	arg1	composite					79:87	glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite	9:87	glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite	9:87	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	0	39	theme	adsorption	126:135	arg1	performance					137:147	Cr(VI) adsorption performance	119:147	Cr(VI) adsorption performance	119:147	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	0	39	theme	adsorption	126:135	arg1	Zr4+					0:3	Zr4+	0:3	Zr4+	0:3	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	9	40	theme	-NH-	1769:1772	arg1	groups					1774:1779	COO- and -NH- groups	1760:1779	COO- and -NH- groups	1760:1779	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	0	41	theme	glutaraldehyde	9:22	arg1	composite					79:87	glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite	9:87	glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite	9:87	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	8	42	theme	electrostatic	1450:1462	arg1	complexation					1504:1515	complexation	1504:1515	complexation	1504:1515	The removal of Cr(VI) mainly involves electrostatic attraction, ion exchange, reduction and complexation.					
37003391	8	42	theme	electrostatic	1450:1462	arg1	reduction					1490:1498	reduction	1490:1498	reduction	1490:1498	The removal of Cr(VI) mainly involves electrostatic attraction, ion exchange, reduction and complexation.					
37003391	8	42	theme	electrostatic	1450:1462	arg1	attraction					1464:1473	electrostatic attraction	1450:1473	electrostatic attraction	1450:1473	The removal of Cr(VI) mainly involves electrostatic attraction, ion exchange, reduction and complexation.					
37003391	8	42	theme	electrostatic	1450:1462	arg1	exchange					1480:1487	ion exchange	1476:1487	ion exchange	1476:1487	The removal of Cr(VI) mainly involves electrostatic attraction, ion exchange, reduction and complexation.					
37003391	6	43	theme	endothermic	1089:1099	arg1	process					1045:1051	the adsorption process	1030:1051	the adsorption process of Cr (VI) by CGPZ	1030:1070	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	6	43	theme	endothermic	1089:1099	arg1	process					1101:1107	a spontaneous endothermic process	1075:1107	a spontaneous endothermic process	1075:1107	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	5	44	theme	removal	916:922	arg1	efficiency					924:933	The removal efficiency	912:933	The removal efficiency of 100 mg L-1 Cr	912:950	The removal efficiency of 100 mg L-1 Cr(VI) reached 95.7 %.					
37003391	5	45	dep	reached	956:962	arg1	VI					952:953	VI	952:953	VI	952:953	The removal efficiency of 100 mg L-1 Cr(VI) reached 95.7 %.					
37003391	0	46	theme	functionalized	55:68	arg1	composite					79:87	glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite	9:87	glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite	9:87	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	9	47	dep	Cr	1670:1671	arg1	%					1684:1684	45.4 %	1679:1684	45.4 %	1679:1684	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	4	48	from	298 K	889:893	arg1	capacity					841:848	The monolayer maximum adsorption capacity	808:848	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ	808:866	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	4	48	from	298 K	889:893	arg1	593.72 mg g-1					872:884	593.72 mg g-1	872:884	593.72 mg g-1	872:884	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	0	49	theme	polyethyleneimine	37:53	arg1	composite					79:87	glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite	9:87	glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite	9:87	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	3	50	theme	ZrN	753:755	arg1	appearance					731:740	the appearance	727:740	the appearance of ZrO and ZrN bonds	727:761	The results showed that polyethyleneimine was successfully grafted onto chitosan by Schiff base reaction, while the appearance of ZrO and ZrN bonds verified the successful preparation of CGPZ.					
37003391	9	51	theme	ion	1603:1605	arg1	exchange					1607:1614	ion exchange	1603:1614	ion exchange	1603:1614	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	6	52	theme	Freundlich	1151:1160	arg1	model					1162:1166	Freundlich model	1151:1166	Freundlich model	1151:1166	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	1	53	theme	electrostatic	213:225	arg1	strategy					316:323	an effective strategy	303:323	an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking	303:422	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	53	theme	electrostatic	213:225	arg1	interaction					227:237	electrostatic interaction	213:237	electrostatic interaction	213:237	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	53	theme	electrostatic	213:225	arg1	it					297:298	it	297:298	it	297:298	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	53	theme	electrostatic	213:225	arg1	ability					256:262	ion exchange ability	243:262	ion exchange ability	243:262	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	53	theme	electrostatic	213:225	arg1	removal					288:294	removal	288:294	removal	288:294	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	4	54	dep	Cr	853:854	arg1	VI					856:857	VI	856:857	VI	856:857	The monolayer maximum adsorption capacity of Cr(VI) by CGPZ was 593.72 mg g-1 at 298 K and t = 210 min.					
37003391	9	55	theme	Zr	1644:1645	arg1	center					1634:1639	the center	1630:1639	the center of Zr	1630:1645	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	9	56	theme	exchange	1607:1614	arg1	interaction					1574:1584	electrostatic interaction	1560:1584	electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion	1560:1625	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	1	57	theme	chitosan	378:385	arg1	chain					397:401	chitosan molecular chain	378:401	chitosan molecular chain through crosslinking	378:422	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	2	58	link	crosslinked	464:474	arg1	composite					525:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	In this paper, Zr4+ and glutaraldehyde crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite was successfully synthesized and characterized by XRD, SEM, FTIR, BET, and XPS.					
37003391	7	59	theme	regeneration	1211:1222	arg1	experiments					1224:1234	The regeneration experiments	1207:1234	The regeneration experiments	1207:1234	The regeneration experiments show that both HCl and NaOH can effectively desorb Cr(III) and Cr(VI) from the adsorbent surface, and the adsorbent has good acid-base resistance and regeneration performance.					
37003391	0	60	theme	chitosan	70:77	arg1	composite					79:87	glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite	9:87	glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite	9:87	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	3	61	theme	ZrO	745:747	arg1	appearance					731:740	the appearance	727:740	the appearance of ZrO and ZrN bonds	727:761	The results showed that polyethyleneimine was successfully grafted onto chitosan by Schiff base reaction, while the appearance of ZrO and ZrN bonds verified the successful preparation of CGPZ.					
37003391	1	62	theme	molecular	387:395	arg1	chain					397:401	chitosan molecular chain	378:401	chitosan molecular chain through crosslinking	378:422	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	6	63	theme	kinetic	1192:1198	arg1	model					1200:1204	pseudo-second-order kinetic model	1172:1204	pseudo-second-order kinetic model	1172:1204	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	9	64	dep	Cr	1660:1661	arg1	VI					1663:1664	VI	1663:1664	VI	1663:1664	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	8	65	theme	ion	1476:1478	arg1	attraction					1464:1473	electrostatic attraction	1450:1473	electrostatic attraction	1450:1473	The removal of Cr(VI) mainly involves electrostatic attraction, ion exchange, reduction and complexation.					
37003391	8	65	theme	ion	1476:1478	arg1	exchange					1480:1487	ion exchange	1476:1487	ion exchange	1476:1487	The removal of Cr(VI) mainly involves electrostatic attraction, ion exchange, reduction and complexation.					
37003391	9	66	theme	Cl-	1619:1621	arg1	ion					1623:1625	Cl- ion	1619:1625	Cl- ion	1619:1625	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	9	67	from	group	1710:1714	arg1	surface					1723:1729	its surface	1719:1729	its surface	1719:1729	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	7	68	theme	adsorbent	1315:1323	arg1	surface					1325:1331	the adsorbent surface	1311:1331	the adsorbent surface	1311:1331	The regeneration experiments show that both HCl and NaOH can effectively desorb Cr(III) and Cr(VI) from the adsorbent surface, and the adsorbent has good acid-base resistance and regeneration performance.					
37003391	3	69	theme	successful	776:785	arg1	preparation					787:797	the successful preparation	772:797	the successful preparation of CGPZ	772:805	The results showed that polyethyleneimine was successfully grafted onto chitosan by Schiff base reaction, while the appearance of ZrO and ZrN bonds verified the successful preparation of CGPZ.					
37003391	6	70	theme	spontaneous	1077:1087	arg1	process					1045:1051	the adsorption process	1030:1051	the adsorption process of Cr (VI) by CGPZ	1030:1070	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	6	70	theme	spontaneous	1077:1087	arg1	process					1101:1107	a spontaneous endothermic process	1075:1107	a spontaneous endothermic process	1075:1107	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	6	71	dep	Cr	1056:1057	arg1	VI					1060:1061	VI	1060:1061	VI	1060:1061	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	6	72	theme	Cr	1056:1057	arg1	process					1045:1051	the adsorption process	1030:1051	the adsorption process of Cr (VI) by CGPZ	1030:1070	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	6	72	theme	Cr	1056:1057	arg1	process					1101:1107	a spontaneous endothermic process	1075:1107	a spontaneous endothermic process	1075:1107	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	6	73	with	accords	1138:1144	arg1	model					1200:1204	pseudo-second-order kinetic model	1172:1204	pseudo-second-order kinetic model	1172:1204	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	6	73	with	accords	1138:1144	arg1	model					1162:1166	Freundlich model	1151:1166	Freundlich model	1151:1166	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	2	74	theme	chitosan	509:516	arg1	composite					525:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	In this paper, Zr4+ and glutaraldehyde crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite was successfully synthesized and characterized by XRD, SEM, FTIR, BET, and XPS.					
37003391	6	75	theme	thermodynamic	976:988	arg1	results					1012:1018	The thermodynamic, isotherm and kinetic results	972:1018	The thermodynamic, isotherm and kinetic results	972:1018	The thermodynamic, isotherm and kinetic results show that the adsorption process of Cr (VI) by CGPZ is a spontaneous endothermic process controlled by entropy, which accords with Freundlich model and pseudo-second-order kinetic model.					
37003391	1	76	theme	ion	243:245	arg1	strategy					316:323	an effective strategy	303:323	an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking	303:422	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	76	theme	ion	243:245	arg1	interaction					227:237	electrostatic interaction	213:237	electrostatic interaction	213:237	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	76	theme	ion	243:245	arg1	it					297:298	it	297:298	it	297:298	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	76	theme	ion	243:245	arg1	removal					288:294	removal	288:294	removal	288:294	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	76	theme	ion	243:245	arg1	ability					256:262	ion exchange ability	243:262	ion exchange ability	243:262	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	2	77	theme	functionalized	494:507	arg1	composite					525:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite	464:533	In this paper, Zr4+ and glutaraldehyde crosslinked polyethyleneimine functionalized chitosan (CGPZ) composite was successfully synthesized and characterized by XRD, SEM, FTIR, BET, and XPS.					
37003391	0	78	dep	Zr4+	0:3	arg1	Zr4+					0:3	Zr4+	0:3	Zr4+	0:3	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	0	78	dep	Zr4+	0:3	arg1	mechanism					150:158	mechanism	150:158	mechanism	150:158	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	0	78	dep	Zr4+	0:3	arg1	regeneration					164:175	regeneration	164:175	regeneration	164:175	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	0	78	dep	Zr4+	0:3	arg1	Synthesis					90:98	Synthesis	90:98	Synthesis	90:98	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	0	78	dep	Zr4+	0:3	arg1	characterization					101:116	characterization	101:116	characterization	101:116	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	0	78	dep	Zr4+	0:3	arg1	performance					137:147	Cr(VI) adsorption performance	119:147	Cr(VI) adsorption performance	119:147	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	9	79	theme	COO-	1760:1763	arg1	groups					1774:1779	COO- and -NH- groups	1760:1779	COO- and -NH- groups	1760:1779	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	0	80	dep	performance	137:147	arg1	VI					122:123	VI	122:123	VI	122:123	Zr4+ and glutaraldehyde cross-linked polyethyleneimine functionalized chitosan composite: Synthesis, characterization, Cr(VI) adsorption performance, mechanism and regeneration.					
37003391	9	81	theme	-NH2/-C=N	1589:1597	arg1	interaction					1574:1584	electrostatic interaction	1560:1584	electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion	1560:1625	CGPZ can synergistically adsorb Cr(VI) by electrostatic interaction of -NH2/-C=N and ion exchange of Cl- ion in the center of Zr, then reduce Cr(VI) to Cr(III) (45.4 % at pH = 2.0) by the -OH group on its surface, and chelate Cr(III) through COO- and -NH- groups.					
37003391	1	82	theme	exchange	247:254	arg1	strategy					316:323	an effective strategy	303:323	an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking	303:422	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	82	theme	exchange	247:254	arg1	interaction					227:237	electrostatic interaction	213:237	electrostatic interaction	213:237	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	82	theme	exchange	247:254	arg1	it					297:298	it	297:298	it	297:298	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	82	theme	exchange	247:254	arg1	removal					288:294	removal	288:294	removal	288:294	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
37003391	1	82	theme	exchange	247:254	arg1	ability					256:262	ion exchange ability	243:262	ion exchange ability	243:262	In order to improve the stability, electrostatic interaction and ion exchange ability of chitosan for Cr (VI) removal, it is an effective strategy to introduce polyvalent metal ions and polymers into chitosan molecular chain through crosslinking.					
35429934	8	0	theme	isolate-carboxymethyl	1065:1085	arg1	cellulose					1087:1095	apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose	1031:1095	apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose	1031:1095	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	4	1	theme	high	551:554	arg1	potential					556:564	high potential	551:564	high potential	551:564	The composite nanoparticles exhibited high potential, small size, and uniform particle size distribution.					
35429934	5	2	theme	neutralization	775:788	arg1	process					790:796	the neutralization process	771:796	the neutralization process	771:796	Circular dichroism and fluorescence spectroscopy results revealed that the nanoparticles showed conformational changes from unfolding to folding during the neutralization process.					
35429934	0	3	theme	pH-driven	85:93	arg1	method					95:100	pH-driven method	85:100	pH-driven method	85:100	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35429934	4	4	theme	small	567:571	arg1	size					573:576	small size	567:576	small size	567:576	The composite nanoparticles exhibited high potential, small size, and uniform particle size distribution.					
35429934	8	5	theme	thermal	1139:1145	arg1	stability					1147:1155	thermal stability	1139:1155	thermal stability	1139:1155	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	5	6	theme	spectroscopy	655:666	arg1	results					668:674	Circular dichroism and fluorescence spectroscopy results	619:674	Circular dichroism and fluorescence spectroscopy results	619:674	Circular dichroism and fluorescence spectroscopy results revealed that the nanoparticles showed conformational changes from unfolding to folding during the neutralization process.					
35429934	6	7	theme	driving	808:814	arg1	forces					816:821	The main driving forces	799:821	The main driving forces for the formation of nanoparticles	799:856	The main driving forces for the formation of nanoparticles were hydrophobic interaction and hydrogen bonding.					
35429934	6	7	theme	driving	808:814	arg1	interaction					875:885	hydrophobic interaction	863:885	hydrophobic interaction	863:885	The main driving forces for the formation of nanoparticles were hydrophobic interaction and hydrogen bonding.					
35429934	8	8	theme	release	1171:1177	arg1	%					1208:1208	65.27%	1203:1208	65.27%	1203:1208	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	8	8	theme	release	1171:1177	arg1	efficiency					1179:1188	in vitro release efficiency	1162:1188	in vitro release efficiency of apigenin (65.27%)	1162:1209	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	4	9	theme	composite	517:525	arg1	nanoparticles					527:539	The composite nanoparticles	513:539	The composite nanoparticles	513:539	The composite nanoparticles exhibited high potential, small size, and uniform particle size distribution.					
35429934	6	10	theme	nanoparticles	844:856	arg1	formation					831:839	the formation	827:839	the formation of nanoparticles	827:856	The main driving forces for the formation of nanoparticles were hydrophobic interaction and hydrogen bonding.					
35429934	6	11	theme	main	803:806	arg1	forces					816:821	The main driving forces	799:821	The main driving forces for the formation of nanoparticles	799:856	The main driving forces for the formation of nanoparticles were hydrophobic interaction and hydrogen bonding.					
35429934	6	11	theme	main	803:806	arg1	interaction					875:885	hydrophobic interaction	863:885	hydrophobic interaction	863:885	The main driving forces for the formation of nanoparticles were hydrophobic interaction and hydrogen bonding.					
35429934	3	12	theme	hydrophilic	451:461	arg1	isolate					476:482	hydrophilic whey protein isolate	451:482	hydrophilic whey protein isolate	451:482	The nanoparticles were stabilized by hydrophilic whey protein isolate and carboxymethyl cellulose.					
35429934	3	13	theme	protein	468:474	arg1	isolate					476:482	hydrophilic whey protein isolate	451:482	hydrophilic whey protein isolate	451:482	The nanoparticles were stabilized by hydrophilic whey protein isolate and carboxymethyl cellulose.					
35429934	8	14	theme	in	1162:1163	arg1	%					1208:1208	65.27%	1203:1208	65.27%	1203:1208	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	8	14	theme	in	1162:1163	arg1	efficiency					1179:1188	in vitro release efficiency	1162:1188	in vitro release efficiency of apigenin (65.27%)	1162:1209	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	5	15	theme	Circular	619:626	arg1	dichroism					628:636	Circular dichroism	619:636	Circular dichroism	619:636	Circular dichroism and fluorescence spectroscopy results revealed that the nanoparticles showed conformational changes from unfolding to folding during the neutralization process.					
35429934	7	16	theme	apigenin	958:965	arg1	capacity					946:953	loading capacity	938:953	loading capacity	938:953	Encapsulation efficiency and loading capacity of apigenin were 87.28% and 8.17%, respectively.					
35429934	7	16	theme	apigenin	958:965	arg1	efficiency					923:932	Encapsulation efficiency	909:932	Encapsulation efficiency	909:932	Encapsulation efficiency and loading capacity of apigenin were 87.28% and 8.17%, respectively.					
35429934	0	17	theme	protein	10:16	arg1	complex					50:56	Zein-whey protein isolate-carboxymethyl cellulose complex	0:56	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.	0:174	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35429934	8	18	dep	in	1162:1163	arg1	vitro					1165:1169	vitro	1165:1169	vitro	1165:1169	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	5	19	theme	dichroism	628:636	arg1	results					668:674	Circular dichroism and fluorescence spectroscopy results	619:674	Circular dichroism and fluorescence spectroscopy results	619:674	Circular dichroism and fluorescence spectroscopy results revealed that the nanoparticles showed conformational changes from unfolding to folding during the neutralization process.					
35429934	1	20	theme	poor	180:183	arg1	solubility					191:200	The poor water solubility	176:200	The poor water solubility of apigenin	176:212	The poor water solubility of apigenin limits its application feasibility in food and medicine.					
35429934	0	21	theme	Zein-whey	0:8	arg1	complex					50:56	Zein-whey protein isolate-carboxymethyl cellulose complex	0:56	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.	0:174	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35429934	4	22	theme	size	600:603	arg1	distribution					605:616	uniform particle size distribution	583:616	uniform particle size distribution	583:616	The composite nanoparticles exhibited high potential, small size, and uniform particle size distribution.					
35429934	5	23	theme	conformational	715:728	arg1	changes					730:736	conformational changes	715:736	conformational changes from unfolding to folding	715:762	Circular dichroism and fluorescence spectroscopy results revealed that the nanoparticles showed conformational changes from unfolding to folding during the neutralization process.					
35429934	3	24	theme	whey	463:466	arg1	isolate					476:482	hydrophilic whey protein isolate	451:482	hydrophilic whey protein isolate	451:482	The nanoparticles were stabilized by hydrophilic whey protein isolate and carboxymethyl cellulose.					
35429934	0	25	theme	cellulose	40:48	arg1	complex					50:56	Zein-whey protein isolate-carboxymethyl cellulose complex	0:56	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.	0:174	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35429934	4	26	theme	particle	591:598	arg1	distribution					605:616	uniform particle size distribution	583:616	uniform particle size distribution	583:616	The composite nanoparticles exhibited high potential, small size, and uniform particle size distribution.					
35429934	8	27	theme	apigenin	1193:1200	arg1	re-dispersibility					1111:1127	better re-dispersibility	1104:1127	better re-dispersibility (92.83%)	1104:1136	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	8	27	theme	apigenin	1193:1200	arg1	%					1135:1135	92.83%	1130:1135	92.83%	1130:1135	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	8	27	theme	apigenin	1193:1200	arg1	stability					1147:1155	thermal stability	1139:1155	thermal stability	1139:1155	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	8	27	theme	apigenin	1193:1200	arg1	%					1208:1208	65.27%	1203:1208	65.27%	1203:1208	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	8	27	theme	apigenin	1193:1200	arg1	efficiency					1179:1188	in vitro release efficiency	1162:1188	in vitro release efficiency of apigenin (65.27%)	1162:1209	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	5	28	theme	fluorescence	642:653	arg1	spectroscopy					655:666	fluorescence spectroscopy	642:666	fluorescence spectroscopy	642:666	Circular dichroism and fluorescence spectroscopy results revealed that the nanoparticles showed conformational changes from unfolding to folding during the neutralization process.					
35429934	8	29	theme	better	1104:1109	arg1	re-dispersibility					1111:1127	better re-dispersibility	1104:1127	better re-dispersibility (92.83%)	1104:1136	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	8	29	theme	better	1104:1109	arg1	%					1135:1135	92.83%	1130:1135	92.83%	1130:1135	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	0	30	theme	isolate-carboxymethyl	18:38	arg1	complex					50:56	Zein-whey protein isolate-carboxymethyl cellulose complex	0:56	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.	0:174	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35429934	7	31	theme	Encapsulation	909:921	arg1	efficiency					923:932	Encapsulation efficiency	909:932	Encapsulation efficiency	909:932	Encapsulation efficiency and loading capacity of apigenin were 87.28% and 8.17%, respectively.					
35429934	7	32	theme	loading	938:944	arg1	capacity					946:953	loading capacity	938:953	loading capacity	938:953	Encapsulation efficiency and loading capacity of apigenin were 87.28% and 8.17%, respectively.					
35429934	2	33	theme	self-assembly	392:404	arg1	method					406:411	the pH-driven self-assembly method	378:411	the pH-driven self-assembly method	378:411	To address this issue, this study successfully prepared apigenin-loaded zein composite nanoparticles using the pH-driven self-assembly method.					
35429934	1	34	theme	apigenin	205:212	arg1	solubility					191:200	The poor water solubility	176:200	The poor water solubility of apigenin	176:212	The poor water solubility of apigenin limits its application feasibility in food and medicine.					
35429934	5	35	from	unfolding	743:751	arg1	changes					730:736	conformational changes	715:736	conformational changes from unfolding to folding	715:762	Circular dichroism and fluorescence spectroscopy results revealed that the nanoparticles showed conformational changes from unfolding to folding during the neutralization process.					
35429934	3	36	theme	carboxymethyl	488:500	arg1	cellulose					502:510	carboxymethyl cellulose	488:510	carboxymethyl cellulose	488:510	The nanoparticles were stabilized by hydrophilic whey protein isolate and carboxymethyl cellulose.					
35429934	1	37	theme	water	185:189	arg1	solubility					191:200	The poor water solubility	176:200	The poor water solubility of apigenin	176:212	The poor water solubility of apigenin limits its application feasibility in food and medicine.					
35429934	2	38	theme	composite	348:356	arg1	nanoparticles					358:370	apigenin-loaded zein composite nanoparticles	327:370	apigenin-loaded zein composite nanoparticles using the pH-driven self-assembly method	327:411	To address this issue, this study successfully prepared apigenin-loaded zein composite nanoparticles using the pH-driven self-assembly method.					
35429934	4	39	theme	uniform	583:589	arg1	distribution					605:616	uniform particle size distribution	583:616	uniform particle size distribution	583:616	The composite nanoparticles exhibited high potential, small size, and uniform particle size distribution.					
35429934	0	40	theme	in	149:150	arg1	property					166:173	in vitro release property	149:173	in vitro release property	149:173	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35429934	0	41	dep	complex	50:56	arg1	Fabrication					103:113	Fabrication	103:113	Fabrication	103:113	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35429934	0	41	dep	complex	50:56	arg1	property					166:173	in vitro release property	149:173	in vitro release property	149:173	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35429934	0	41	dep	complex	50:56	arg1	stability					134:142	stability	134:142	stability	134:142	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35429934	0	41	dep	complex	50:56	arg1	characterization					116:131	characterization	116:131	characterization	116:131	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35429934	8	42	theme	apigenin-loaded	1031:1045	arg1	cellulose					1087:1095	apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose	1031:1095	apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose	1031:1095	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	2	43	theme	zein	343:346	arg1	nanoparticles					358:370	apigenin-loaded zein composite nanoparticles	327:370	apigenin-loaded zein composite nanoparticles using the pH-driven self-assembly method	327:411	To address this issue, this study successfully prepared apigenin-loaded zein composite nanoparticles using the pH-driven self-assembly method.					
35429934	8	44	theme	single	1018:1023	arg1	zein					1025:1028	single zein	1018:1028	single zein	1018:1028	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	6	45	theme	hydrophobic	863:873	arg1	interaction					875:885	hydrophobic interaction	863:885	hydrophobic interaction	863:885	The main driving forces for the formation of nanoparticles were hydrophobic interaction and hydrogen bonding.					
35429934	6	45	theme	hydrophobic	863:873	arg1	forces					816:821	The main driving forces	799:821	The main driving forces for the formation of nanoparticles	799:856	The main driving forces for the formation of nanoparticles were hydrophobic interaction and hydrogen bonding.					
35429934	6	46	theme	hydrogen	891:898	arg1	bonding					900:906	hydrogen bonding	891:906	hydrogen bonding	891:906	The main driving forces for the formation of nanoparticles were hydrophobic interaction and hydrogen bonding.					
35429934	2	47	theme	apigenin-loaded	327:341	arg1	nanoparticles					358:370	apigenin-loaded zein composite nanoparticles	327:370	apigenin-loaded zein composite nanoparticles using the pH-driven self-assembly method	327:411	To address this issue, this study successfully prepared apigenin-loaded zein composite nanoparticles using the pH-driven self-assembly method.					
35429934	0	48	theme	release	158:164	arg1	property					166:173	in vitro release property	149:173	in vitro release property	149:173	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35429934	8	49	theme	protein	1057:1063	arg1	cellulose					1087:1095	apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose	1031:1095	apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose	1031:1095	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	0	50	dep	in	149:150	arg1	vitro					152:156	vitro	152:156	vitro	152:156	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35429934	1	51	theme	application	225:235	arg1	feasibility					237:247	its application feasibility	221:247	its application feasibility	221:247	The poor water solubility of apigenin limits its application feasibility in food and medicine.					
35429934	8	52	theme	zein-whey	1047:1055	arg1	cellulose					1087:1095	apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose	1031:1095	apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose	1031:1095	Compared with single zein, apigenin-loaded zein-whey protein isolate-carboxymethyl cellulose showed better re-dispersibility (92.83%), thermal stability, and in vitro release efficiency of apigenin (65.27%).					
35429934	2	53	theme	pH-driven	382:390	arg1	method					406:411	the pH-driven self-assembly method	378:411	the pH-driven self-assembly method	378:411	To address this issue, this study successfully prepared apigenin-loaded zein composite nanoparticles using the pH-driven self-assembly method.					
35429934	0	54	theme	apigenin	72:79	arg1	carrier					61:67	carrier	61:67	carrier of apigenin	61:79	Zein-whey protein isolate-carboxymethyl cellulose complex as carrier of apigenin via pH-driven method: Fabrication, characterization, stability, and in vitro release property.					
35460758	8	0	theme	antibacterial	1462:1474	arg1	ability					1476:1482	their antibacterial ability	1456:1482	their antibacterial ability against Staphylococcus aureus and Escherichia coli	1456:1533	Additionally, the carriers were endowed with antioxidant activity, and their antibacterial ability against Staphylococcus aureus and Escherichia coli was pOVA-KAE-TA-SA > pOVA-KAE-SA > pOVA-KAE > pOVA-SA > pOVA based on the difference in antibacterial diameter (D, mm) and square (S, mm2).					
35460758	8	1	dep	diameter	1637:1644	arg1	D					1647:1647	D	1647:1647	D	1647:1647	Additionally, the carriers were endowed with antioxidant activity, and their antibacterial ability against Staphylococcus aureus and Escherichia coli was pOVA-KAE-TA-SA > pOVA-KAE-SA > pOVA-KAE > pOVA-SA > pOVA based on the difference in antibacterial diameter (D, mm) and square (S, mm2).					
35460758	8	1	dep	diameter	1637:1644	arg1	mm					1650:1651	mm	1650:1651	mm	1650:1651	Additionally, the carriers were endowed with antioxidant activity, and their antibacterial ability against Staphylococcus aureus and Escherichia coli was pOVA-KAE-TA-SA > pOVA-KAE-SA > pOVA-KAE > pOVA-SA > pOVA based on the difference in antibacterial diameter (D, mm) and square (S, mm2).					
35460758	1	2	theme	OVA-SA	236:241	arg1	carriers					253:260	OVA-SA composite carriers	236:260	OVA-SA composite carriers	236:260	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	5	3	theme	redshift	1087:1094	arg1	degrees					1076:1082	the different degrees	1062:1082	the different degrees of redshift of the composite nanoparticle λmax	1062:1129	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	6	4	theme	structure	1238:1246	arg1	stability					1262:1270	the protein structure the strongest stability	1226:1270	the protein structure the strongest stability	1226:1270	Additionally, pOVA-SA had the highest α-helix content and the lowest random coils, conferring the protein structure the strongest stability.					
35460758	3	5	from	peaks	508:512	arg1	carriers					517:524	carriers	517:524	carriers	517:524	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	1	6	theme	composite	243:251	arg1	carriers					253:260	OVA-SA composite carriers	236:260	OVA-SA composite carriers	236:260	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	0	7	theme	acid	133:136	arg1	encapsulation					98:110	the encapsulation	94:110	the encapsulation of kaempferol/tannin acid	94:136	Influence of polyphenol-metal ion-coated ovalbumin/sodium alginate composite nanoparticles on the encapsulation of kaempferol/tannin acid.					
35460758	6	8	theme	highest	1162:1168	arg1	content					1178:1184	α-helix content	1170:1184	α-helix content	1170:1184	Additionally, pOVA-SA had the highest α-helix content and the lowest random coils, conferring the protein structure the strongest stability.					
35460758	6	9	theme	protein	1230:1236	arg1	stability					1262:1270	the protein structure the strongest stability	1226:1270	the protein structure the strongest stability	1226:1270	Additionally, pOVA-SA had the highest α-helix content and the lowest random coils, conferring the protein structure the strongest stability.					
35460758	3	10	theme	peaks	508:512	arg1	observation					475:485	the observation	471:485	the observation of small diffraction peaks in carriers	471:524	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	6	11	theme	lowest	1194:1199	arg1	coils					1208:1212	the lowest random coils	1190:1212	the lowest random coils	1190:1212	Additionally, pOVA-SA had the highest α-helix content and the lowest random coils, conferring the protein structure the strongest stability.					
35460758	0	12	theme	kaempferol/tannin	115:131	arg1	acid					133:136	kaempferol/tannin acid	115:136	kaempferol/tannin acid	115:136	Influence of polyphenol-metal ion-coated ovalbumin/sodium alginate composite nanoparticles on the encapsulation of kaempferol/tannin acid.					
35460758	1	13	theme	hydrophilic	337:347	arg1	TA					362:363	TA	362:363	TA	362:363	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	1	13	theme	hydrophilic	337:347	arg1	acid					356:359	the hydrophilic tannic acid	333:359	the hydrophilic tannic acid (TA)	333:364	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	5	14	theme	opposite	942:949	arg1	position					951:958	the opposite position	938:958	the opposite position in the benzene ring of TA	938:984	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	3	15	from	observation	475:485	arg1	carriers					517:524	carriers	517:524	carriers	517:524	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	4	16	theme	nanoparticles	707:719	arg1	structure					680:688	The protein structure	668:688	The protein structure of the composite nanoparticles coated with TA/Fe3+	668:739	The protein structure of the composite nanoparticles coated with TA/Fe3+ changed, and the order of the changes was pOVA-KAE > pOVA > pOVA-KAE-SA > pOVA-KAE-TA-SA > pOVA-SA.					
35460758	2	17	theme	delivery	402:409	arg1	purpose					382:388	the purpose	378:388	the purpose of targeted delivery	378:409	To achieve the purpose of targeted delivery, the TA-Fe3+ coating film was prepared.					
35460758	7	18	theme	nanoparticles	1370:1382	arg1	stability					1317:1325	the system stability	1306:1325	the system stability	1306:1325	The coating of TA/Fe3+ increased the system stability and the thermal stability of the composite nanoparticles.					
35460758	7	18	theme	nanoparticles	1370:1382	arg1	stability					1343:1351	the thermal stability	1331:1351	the thermal stability	1331:1351	The coating of TA/Fe3+ increased the system stability and the thermal stability of the composite nanoparticles.					
35460758	1	19	theme	tannic	349:354	arg1	TA					362:363	TA	362:363	TA	362:363	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	1	19	theme	tannic	349:354	arg1	acid					356:359	the hydrophilic tannic acid	333:359	the hydrophilic tannic acid (TA)	333:364	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	3	20	theme	small	490:494	arg1	peaks					508:512	small diffraction peaks	490:512	small diffraction peaks in carriers	490:524	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	1	21	theme	sodium	177:182	arg1	SA					194:195	SA	194:195	SA	194:195	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	1	21	theme	sodium	177:182	arg1	ovalbumin					157:165	ovalbumin	157:165	ovalbumin (OVA)	157:171	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	1	21	theme	sodium	177:182	arg1	alginate					184:191	sodium alginate	177:191	sodium alginate (SA)	177:196	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	1	21	theme	sodium	177:182	arg1	materials					215:223	the materials	211:223	the materials	211:223	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	0	22	theme	composite	67:75	arg1	Influence					0:8	Influence	0:8	Influence of polyphenol-metal ion-coated ovalbumin/sodium alginate composite	0:75	Influence of polyphenol-metal ion-coated ovalbumin/sodium alginate composite nanoparticles on the encapsulation of kaempferol/tannin acid.					
35460758	3	23	theme	diffraction	496:506	arg1	peaks					508:512	small diffraction peaks	490:512	small diffraction peaks in carriers	490:524	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	8	24	theme	antibacterial	1623:1635	arg1	diameter					1637:1644	antibacterial diameter	1623:1644	antibacterial diameter (D, mm)	1623:1652	Additionally, the carriers were endowed with antioxidant activity, and their antibacterial ability against Staphylococcus aureus and Escherichia coli was pOVA-KAE-TA-SA > pOVA-KAE-SA > pOVA-KAE > pOVA-SA > pOVA based on the difference in antibacterial diameter (D, mm) and square (S, mm2).					
35460758	5	25	theme	different	1066:1074	arg1	degrees					1076:1082	the different degrees	1062:1082	the different degrees of redshift of the composite nanoparticle λmax	1062:1129	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	8	26	from	difference	1609:1618	arg1	diameter					1637:1644	antibacterial diameter	1623:1644	antibacterial diameter (D, mm)	1623:1652	Additionally, the carriers were endowed with antioxidant activity, and their antibacterial ability against Staphylococcus aureus and Escherichia coli was pOVA-KAE-TA-SA > pOVA-KAE-SA > pOVA-KAE > pOVA-SA > pOVA based on the difference in antibacterial diameter (D, mm) and square (S, mm2).					
35460758	8	26	from	difference	1609:1618	arg1	square					1658:1663	square	1658:1663	square (S, mm2)	1658:1672	Additionally, the carriers were endowed with antioxidant activity, and their antibacterial ability against Staphylococcus aureus and Escherichia coli was pOVA-KAE-TA-SA > pOVA-KAE-SA > pOVA-KAE > pOVA-SA > pOVA based on the difference in antibacterial diameter (D, mm) and square (S, mm2).					
35460758	5	27	theme	auxo-chromophore	910:925	arg1	-OH					927:929	the auxo-chromophore -OH	906:929	the auxo-chromophore -OH	906:929	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	10	28	theme	compounds	1927:1935	arg1	delivery					1905:1912	the delivery	1901:1912	the delivery of bioactive compounds	1901:1935	These results provide a theoretical basis for the application of OVA-SA composite nanoparticles in the delivery of bioactive compounds.					
35460758	6	29	theme	random	1201:1206	arg1	coils					1208:1212	the lowest random coils	1190:1212	the lowest random coils	1190:1212	Additionally, pOVA-SA had the highest α-helix content and the lowest random coils, conferring the protein structure the strongest stability.					
35460758	0	30	theme	ion-coated	30:39	arg1	composite					67:75	polyphenol-metal ion-coated ovalbumin/sodium alginate composite	13:75	polyphenol-metal ion-coated ovalbumin/sodium alginate composite	13:75	Influence of polyphenol-metal ion-coated ovalbumin/sodium alginate composite nanoparticles on the encapsulation of kaempferol/tannin acid.					
35460758	9	31	theme	TA/KAE	1794:1799	arg1	bioavailability					1775:1789	the bioavailability	1771:1789	the bioavailability of TA/KAE	1771:1799	pOVA-KAE-TA-SA had the strongest antioxidant activity and antibacterial ability, which improved the bioavailability of TA/KAE.					
35460758	7	32	theme	system	1310:1315	arg1	stability					1317:1325	the system stability	1306:1325	the system stability	1306:1325	The coating of TA/Fe3+ increased the system stability and the thermal stability of the composite nanoparticles.					
35460758	4	33	theme	composite	697:705	arg1	nanoparticles					707:719	the composite nanoparticles	693:719	the composite nanoparticles coated with TA/Fe3+	693:739	The protein structure of the composite nanoparticles coated with TA/Fe3+ changed, and the order of the changes was pOVA-KAE > pOVA > pOVA-KAE-SA > pOVA-KAE-TA-SA > pOVA-SA.					
35460758	5	34	theme	TA	983:984	arg1	ring					975:978	the benzene ring	963:978	the benzene ring of TA	963:984	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	9	35	contain	had	1690:1692	arg2	activity					1720:1727	antioxidant activity	1708:1727	antioxidant activity	1708:1727	pOVA-KAE-TA-SA had the strongest antioxidant activity and antibacterial ability, which improved the bioavailability of TA/KAE.					
35460758	9	35	contain	had	1690:1692	arg2	ability					1747:1753	antibacterial ability	1733:1753	antibacterial ability	1733:1753	pOVA-KAE-TA-SA had the strongest antioxidant activity and antibacterial ability, which improved the bioavailability of TA/KAE.					
35460758	9	35	contain	had	1690:1692	arg1	pOVA-KAE-TA-SA					1675:1688	pOVA-KAE-TA-SA	1675:1688	pOVA-KAE-TA-SA	1675:1688	pOVA-KAE-TA-SA had the strongest antioxidant activity and antibacterial ability, which improved the bioavailability of TA/KAE.					
35460758	2	36	theme	targeted	393:400	arg1	delivery					402:409	targeted delivery	393:409	targeted delivery	393:409	To achieve the purpose of targeted delivery, the TA-Fe3+ coating film was prepared.					
35460758	10	37	theme	nanoparticles	1884:1896	arg1	application					1852:1862	the application	1848:1862	the application of OVA-SA composite nanoparticles in the delivery of bioactive compounds	1848:1935	These results provide a theoretical basis for the application of OVA-SA composite nanoparticles in the delivery of bioactive compounds.					
35460758	9	38	theme	strongest	1698:1706	arg1	activity					1720:1727	antioxidant activity	1708:1727	antioxidant activity	1708:1727	pOVA-KAE-TA-SA had the strongest antioxidant activity and antibacterial ability, which improved the bioavailability of TA/KAE.					
35460758	3	39	theme	nanoparticles	604:616	arg1	surface					578:584	the surface	574:584	the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA)	574:665	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	7	40	theme	composite	1360:1368	arg1	nanoparticles					1370:1382	the composite nanoparticles	1356:1382	the composite nanoparticles	1356:1382	The coating of TA/Fe3+ increased the system stability and the thermal stability of the composite nanoparticles.					
35460758	10	41	from	application	1852:1862	arg1	delivery					1905:1912	the delivery	1901:1912	the delivery of bioactive compounds	1901:1935	These results provide a theoretical basis for the application of OVA-SA composite nanoparticles in the delivery of bioactive compounds.					
35460758	0	42	theme	alginate	58:65	arg1	composite					67:75	polyphenol-metal ion-coated ovalbumin/sodium alginate composite	13:75	polyphenol-metal ion-coated ovalbumin/sodium alginate composite	13:75	Influence of polyphenol-metal ion-coated ovalbumin/sodium alginate composite nanoparticles on the encapsulation of kaempferol/tannin acid.					
35460758	9	43	theme	antioxidant	1708:1718	arg1	activity					1720:1727	antioxidant activity	1708:1727	antioxidant activity	1708:1727	pOVA-KAE-TA-SA had the strongest antioxidant activity and antibacterial ability, which improved the bioavailability of TA/KAE.					
35460758	10	44	theme	composite	1874:1882	arg1	nanoparticles					1884:1896	OVA-SA composite nanoparticles	1867:1896	OVA-SA composite nanoparticles	1867:1896	These results provide a theoretical basis for the application of OVA-SA composite nanoparticles in the delivery of bioactive compounds.					
35460758	3	45	theme	coating	558:564	arg1	film					566:569	TA/Fe3+ coating film	550:569	TA/Fe3+ coating film	550:569	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	0	46	theme	ovalbumin/sodium	41:56	arg1	composite					67:75	polyphenol-metal ion-coated ovalbumin/sodium alginate composite	13:75	polyphenol-metal ion-coated ovalbumin/sodium alginate composite	13:75	Influence of polyphenol-metal ion-coated ovalbumin/sodium alginate composite nanoparticles on the encapsulation of kaempferol/tannin acid.					
35460758	6	47	contain	had	1154:1156	arg2	content					1178:1184	α-helix content	1170:1184	α-helix content	1170:1184	Additionally, pOVA-SA had the highest α-helix content and the lowest random coils, conferring the protein structure the strongest stability.					
35460758	6	47	contain	had	1154:1156	arg2	coils					1208:1212	the lowest random coils	1190:1212	the lowest random coils	1190:1212	Additionally, pOVA-SA had the highest α-helix content and the lowest random coils, conferring the protein structure the strongest stability.					
35460758	6	47	contain	had	1154:1156	arg1	pOVA-SA					1146:1152	pOVA-SA	1146:1152	pOVA-SA	1146:1152	Additionally, pOVA-SA had the highest α-helix content and the lowest random coils, conferring the protein structure the strongest stability.					
35460758	5	48	contain	have	1012:1015	arg1	substituents					999:1010	the two substituents	991:1010	the two substituents	991:1010	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	5	48	contain	have	1012:1015	arg2	effects					1026:1032	opposite effects	1017:1032	opposite effects	1017:1032	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	7	49	theme	TA/Fe3+	1288:1294	arg1	coating					1277:1283	The coating	1273:1283	The coating of TA/Fe3+	1273:1294	The coating of TA/Fe3+ increased the system stability and the thermal stability of the composite nanoparticles.					
35460758	3	50	theme	film	566:569	arg1	formation					537:545	the formation	533:545	the formation of TA/Fe3+ coating film	533:569	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	7	51	theme	thermal	1335:1341	arg1	stability					1343:1351	the thermal stability	1331:1351	the thermal stability	1331:1351	The coating of TA/Fe3+ increased the system stability and the thermal stability of the composite nanoparticles.					
35460758	2	52	theme	coating	424:430	arg1	film					432:435	the TA-Fe3+ coating film	412:435	the TA-Fe3+ coating film	412:435	To achieve the purpose of targeted delivery, the TA-Fe3+ coating film was prepared.					
35460758	1	53	theme	hydrophobic	300:310	arg1	KAE					324:326	KAE	324:326	KAE	324:326	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	1	53	theme	hydrophobic	300:310	arg1	kaempferol					312:321	the hydrophobic kaempferol	296:321	the hydrophobic kaempferol (KAE)	296:327	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	3	54	theme	composite	594:602	arg1	nanoparticles					604:616	four composite nanoparticles	589:616	four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA)	589:665	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	3	54	theme	composite	594:602	arg1	pOVA-KAE-SA					634:644	pOVA-KAE-SA	634:644	pOVA-KAE-SA	634:644	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	3	54	theme	composite	594:602	arg1	pOVA-KAE-TA-SA					651:664	pOVA-KAE-TA-SA	651:664	pOVA-KAE-TA-SA	651:664	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	3	54	theme	composite	594:602	arg1	pOVA-SA					625:631	pOVA-SA	625:631	pOVA-SA	625:631	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	3	54	theme	composite	594:602	arg1	pOVA					619:622	pOVA	619:622	pOVA	619:622	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	4	55	theme	changes	771:777	arg1	pOVA-KAE > pOVA > pOVA-KAE-SA > pOVA-KAE-TA-SA > pOVA-SA					783:838	pOVA-KAE > pOVA > pOVA-KAE-SA > pOVA-KAE-TA-SA > pOVA-SA	783:838	pOVA-KAE > pOVA > pOVA-KAE-SA > pOVA-KAE-TA-SA > pOVA-SA	783:838	The protein structure of the composite nanoparticles coated with TA/Fe3+ changed, and the order of the changes was pOVA-KAE > pOVA > pOVA-KAE-SA > pOVA-KAE-TA-SA > pOVA-SA.					
35460758	4	55	theme	changes	771:777	arg1	order					758:762	the order	754:762	the order of the changes	754:777	The protein structure of the composite nanoparticles coated with TA/Fe3+ changed, and the order of the changes was pOVA-KAE > pOVA > pOVA-KAE-SA > pOVA-KAE-TA-SA > pOVA-SA.					
35460758	5	56	theme	chromophore	885:895	arg1	-C=O					897:900	the chromophore -C=O	881:900	the chromophore -C=O	881:900	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	2	57	theme	TA-Fe3+	416:422	arg1	film					432:435	the TA-Fe3+ coating film	412:435	the TA-Fe3+ coating film	412:435	To achieve the purpose of targeted delivery, the TA-Fe3+ coating film was prepared.					
35460758	5	58	theme	nanoparticle	1113:1124	arg1	λmax					1126:1129	the composite nanoparticle λmax	1099:1129	the composite nanoparticle λmax	1099:1129	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	9	59	theme	antibacterial	1733:1745	arg1	ability					1747:1753	antibacterial ability	1733:1753	antibacterial ability	1733:1753	pOVA-KAE-TA-SA had the strongest antioxidant activity and antibacterial ability, which improved the bioavailability of TA/KAE.					
35460758	3	60	theme	TA/Fe3+	550:556	arg1	film					566:569	TA/Fe3+ coating film	550:569	TA/Fe3+ coating film	550:569	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	10	61	theme	bioactive	1917:1925	arg1	compounds					1927:1935	bioactive compounds	1917:1935	bioactive compounds	1917:1935	These results provide a theoretical basis for the application of OVA-SA composite nanoparticles in the delivery of bioactive compounds.					
35460758	8	62	theme	antioxidant	1430:1440	arg1	activity					1442:1449	antioxidant activity	1430:1449	antioxidant activity	1430:1449	Additionally, the carriers were endowed with antioxidant activity, and their antibacterial ability against Staphylococcus aureus and Escherichia coli was pOVA-KAE-TA-SA > pOVA-KAE-SA > pOVA-KAE > pOVA-SA > pOVA based on the difference in antibacterial diameter (D, mm) and square (S, mm2).					
35460758	3	63	from	carriers	517:524	arg1	observation					475:485	the observation	471:485	the observation of small diffraction peaks in carriers	471:524	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	5	64	theme	λmax	1126:1129	arg1	redshift					1087:1094	redshift	1087:1094	redshift of the composite nanoparticle λmax	1087:1129	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	6	65	theme	α-helix	1170:1176	arg1	content					1178:1184	α-helix content	1170:1184	α-helix content	1170:1184	Additionally, pOVA-SA had the highest α-helix content and the lowest random coils, conferring the protein structure the strongest stability.					
35460758	1	66	used	used	203:206	arg2	SA					194:195	SA	194:195	SA	194:195	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	1	66	used	used	203:206	arg2	OVA					168:170	OVA	168:170	OVA	168:170	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	1	66	used	used	203:206	arg2	ovalbumin					157:165	ovalbumin	157:165	ovalbumin (OVA)	157:171	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	1	66	used	used	203:206	arg2	materials					215:223	the materials	211:223	the materials	211:223	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	1	66	used	used	203:206	arg2	alginate					184:191	sodium alginate	177:191	sodium alginate (SA)	177:196	In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA).					
35460758	4	67	theme	protein	672:678	arg1	structure					680:688	The protein structure	668:688	The protein structure of the composite nanoparticles coated with TA/Fe3+	668:739	The protein structure of the composite nanoparticles coated with TA/Fe3+ changed, and the order of the changes was pOVA-KAE > pOVA > pOVA-KAE-SA > pOVA-KAE-TA-SA > pOVA-SA.					
35460758	6	68	theme	strongest	1252:1260	arg1	stability					1262:1270	the protein structure the strongest stability	1226:1270	the protein structure the strongest stability	1226:1270	Additionally, pOVA-SA had the highest α-helix content and the lowest random coils, conferring the protein structure the strongest stability.					
35460758	5	69	theme	benzene	967:973	arg1	ring					975:978	the benzene ring	963:978	the benzene ring of TA	963:984	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	8	70	with	endowed	1417:1423	arg1	activity					1442:1449	antioxidant activity	1430:1449	antioxidant activity	1430:1449	Additionally, the carriers were endowed with antioxidant activity, and their antibacterial ability against Staphylococcus aureus and Escherichia coli was pOVA-KAE-TA-SA > pOVA-KAE-SA > pOVA-KAE > pOVA-SA > pOVA based on the difference in antibacterial diameter (D, mm) and square (S, mm2).					
35460758	5	71	theme	opposite	1017:1024	arg1	effects					1026:1032	opposite effects	1017:1032	opposite effects	1017:1032	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	10	72	theme	OVA-SA	1867:1872	arg1	nanoparticles					1884:1896	OVA-SA composite nanoparticles	1867:1896	OVA-SA composite nanoparticles	1867:1896	These results provide a theoretical basis for the application of OVA-SA composite nanoparticles in the delivery of bioactive compounds.					
35460758	3	73	dep	nanoparticles	604:616	arg1	nanoparticles					604:616	four composite nanoparticles	589:616	four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA)	589:665	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	3	73	dep	nanoparticles	604:616	arg1	pOVA-KAE-SA					634:644	pOVA-KAE-SA	634:644	pOVA-KAE-SA	634:644	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	3	73	dep	nanoparticles	604:616	arg1	pOVA-KAE-TA-SA					651:664	pOVA-KAE-TA-SA	651:664	pOVA-KAE-TA-SA	651:664	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	3	73	dep	nanoparticles	604:616	arg1	pOVA-SA					625:631	pOVA-SA	625:631	pOVA-SA	625:631	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	3	73	dep	nanoparticles	604:616	arg1	pOVA					619:622	pOVA	619:622	pOVA	619:622	Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA).					
35460758	8	74	dep	square	1658:1663	arg1	mm2					1669:1671	mm2	1669:1671	mm2	1669:1671	Additionally, the carriers were endowed with antioxidant activity, and their antibacterial ability against Staphylococcus aureus and Escherichia coli was pOVA-KAE-TA-SA > pOVA-KAE-SA > pOVA-KAE > pOVA-SA > pOVA based on the difference in antibacterial diameter (D, mm) and square (S, mm2).					
35460758	8	74	dep	square	1658:1663	arg1	S					1666:1666	S	1666:1666	S	1666:1666	Additionally, the carriers were endowed with antioxidant activity, and their antibacterial ability against Staphylococcus aureus and Escherichia coli was pOVA-KAE-TA-SA > pOVA-KAE-SA > pOVA-KAE > pOVA-SA > pOVA based on the difference in antibacterial diameter (D, mm) and square (S, mm2).					
35460758	0	75	theme	polyphenol-metal	13:28	arg1	composite					67:75	polyphenol-metal ion-coated ovalbumin/sodium alginate composite	13:75	polyphenol-metal ion-coated ovalbumin/sodium alginate composite	13:75	Influence of polyphenol-metal ion-coated ovalbumin/sodium alginate composite nanoparticles on the encapsulation of kaempferol/tannin acid.					
35460758	10	76	theme	theoretical	1826:1836	arg1	basis					1838:1842	a theoretical basis	1824:1842	a theoretical basis for the application of OVA-SA composite nanoparticles in the delivery of bioactive compounds	1824:1935	These results provide a theoretical basis for the application of OVA-SA composite nanoparticles in the delivery of bioactive compounds.					
35460758	6	77	dep	content	1178:1184	arg1	the					1158:1160	the	1158:1160	the	1158:1160	Additionally, pOVA-SA had the highest α-helix content and the lowest random coils, conferring the protein structure the strongest stability.					
35460758	5	78	from	position	951:958	arg1	ring					975:978	the benzene ring	963:978	the benzene ring of TA	963:984	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
35460758	5	79	theme	composite	1103:1111	arg1	λmax					1126:1129	the composite nanoparticle λmax	1099:1129	the composite nanoparticle λmax	1099:1129	This phenomenon is due to the fact that the chromophore -C=O and the auxo-chromophore -OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax.					
36459855	7	0	theme	optimized	1167:1175	arg1	conditions					1177:1186	the optimized conditions	1163:1186	the optimized conditions	1163:1186	Under the optimized conditions, the limit of detection and quantification for the target analytes were estimated to be in the range of 0.003-0.035 and 0.010-0.117 µg mL-1, respectively.					
36459855	3	1	theme	unmodified	651:660	arg1	paper					672:676	the unmodified cellulose paper	647:676	the unmodified cellulose paper followed by elution into 2 mL of methanol	647:718	It involves adsorption of target analytes from deproteinized diluted blood samples on the unmodified cellulose paper followed by elution into 2 mL of methanol.					
36459855	1	2	from	extraction	291:300	arg1	samples					398:404	human blood samples	386:404	human blood samples	386:404	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	1	3	theme	blood	392:396	arg1	samples					398:404	human blood samples	386:404	human blood samples	386:404	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	11	4	theme	method	1771:1776	arg1	application					1743:1753	the potential application	1729:1753	the potential application of the proposed method	1729:1776	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	9	5	from	range	1461:1465	arg1	curves					1435:1440	The calibration curves	1419:1440	The calibration curves	1419:1440	The calibration curves were linear in the range of 0.015-2 µg mL-1 with a coefficient of determination (R2) in the range of 0.995-0.999.					
36459855	9	5	from	range	1461:1465	arg1	linear					1447:1452	linear	1447:1452	linear	1447:1452	The calibration curves were linear in the range of 0.015-2 µg mL-1 with a coefficient of determination (R2) in the range of 0.995-0.999.					
36459855	6	6	theme	MeOH	1112:1115	arg1	2 mL					1104:1107	only 2 mL	1099:1107	only 2 mL of MeOH	1099:1115	The technique is easy to perform and requires only 2 mL of MeOH during the entire extraction procedure.					
36459855	11	7	from	patients	1691:1698	arg1	drugs					1659:1663	nine drugs	1654:1663	nine drugs in blood samples from the patients	1654:1698	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	11	7	from	patients	1691:1698	arg1	samples					1674:1680	blood samples	1668:1680	blood samples from the patients	1668:1698	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	1	8	theme	unmodified	241:250	arg1	paper					270:274	unmodified laboratory filter paper	241:274	unmodified laboratory filter paper	241:274	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	1	9	theme	gas	428:430	arg1	GC-MS					466:470	GC-MS	466:470	GC-MS	466:470	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	1	9	theme	gas	428:430	arg1	spectrometry					452:463	gas chromatography-mass spectrometry	428:463	gas chromatography-mass spectrometry (GC-MS)	428:471	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	2	10	theme	sorptive	514:521	arg1	CPSE					535:538	CPSE	535:538	CPSE	535:538	The procedure termed as cellulose paper sorptive extraction (CPSE) is straightforward.					
36459855	2	10	theme	sorptive	514:521	arg1	extraction					523:532	sorptive extraction	514:532	cellulose paper sorptive extraction (CPSE)	498:539	The procedure termed as cellulose paper sorptive extraction (CPSE) is straightforward.					
36459855	2	11	theme	cellulose	498:506	arg1	paper					508:512	cellulose paper sorptive extraction (CPSE)	498:539	cellulose paper sorptive extraction (CPSE)	498:539	The procedure termed as cellulose paper sorptive extraction (CPSE) is straightforward.					
36459855	8	12	theme	standard	1369:1376	arg1	deviations					1378:1387	the relative standard deviations	1356:1387	the relative standard deviations	1356:1387	In contrast, the relative standard deviations were consistently below 10 %.					
36459855	0	13	theme	drugs	113:117	arg1	analysis					95:102	analysis	95:102	analysis of basic drugs in blood as a proof of concept	95:148	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	1	14	theme	preparation	185:195	arg1	procedure					197:205	sample preparation procedure	178:205	sample preparation procedure	178:205	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	5	15	theme	unmodified	975:984	arg1	paper					993:997	the unmodified filter paper	971:997	the unmodified filter paper used as the sorbent	971:1017	The proposed method follows the principles of green analytical chemistry (GAC), as the unmodified filter paper used as the sorbent is inexpensive and biodegradable.					
36459855	5	15	theme	unmodified	975:984	arg1	inexpensive					1022:1032	inexpensive	1022:1032	inexpensive	1022:1032	The proposed method follows the principles of green analytical chemistry (GAC), as the unmodified filter paper used as the sorbent is inexpensive and biodegradable.					
36459855	9	16	theme	calibration	1423:1433	arg1	curves					1435:1440	The calibration curves	1419:1440	The calibration curves	1419:1440	The calibration curves were linear in the range of 0.015-2 µg mL-1 with a coefficient of determination (R2) in the range of 0.995-0.999.					
36459855	9	16	theme	calibration	1423:1433	arg1	linear					1447:1452	linear	1447:1452	linear	1447:1452	The calibration curves were linear in the range of 0.015-2 µg mL-1 with a coefficient of determination (R2) in the range of 0.995-0.999.					
36459855	4	17	theme	significant	858:868	arg1	factors					870:876	significant factors	858:876	significant factors for CPSE	858:885	Multivariate optimization, consisting of Placket-Burman design (PBD) and central composite design (CCD), was used to screen and optimize significant factors for CPSE.					
36459855	6	18	theme	extraction	1135:1144	arg1	procedure					1146:1154	the entire extraction procedure	1124:1154	the entire extraction procedure	1124:1154	The technique is easy to perform and requires only 2 mL of MeOH during the entire extraction procedure.					
36459855	3	19	theme	target	587:592	arg1	analytes					594:601	target analytes	587:601	target analytes	587:601	It involves adsorption of target analytes from deproteinized diluted blood samples on the unmodified cellulose paper followed by elution into 2 mL of methanol.					
36459855	11	20	theme	blood	1668:1672	arg1	samples					1674:1680	blood samples	1668:1680	blood samples from the patients	1668:1698	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	1	21	theme	basic	310:314	arg1	drugs					316:320	nine basic drugs	305:320	nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples	305:404	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	9	22	with	linear	1447:1452	arg1	coefficient					1493:1503	a coefficient	1491:1503	a coefficient of determination (R2)	1491:1525	The calibration curves were linear in the range of 0.015-2 µg mL-1 with a coefficient of determination (R2) in the range of 0.995-0.999.					
36459855	11	23	theme	drugs	1659:1663	arg1	analysis					1642:1649	the analysis	1638:1649	the analysis of nine drugs in blood samples from the patients	1638:1698	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	11	23	theme	drugs	1659:1663	arg1	proof					1620:1624	proof	1620:1624	proof of concept	1620:1635	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	0	24	theme	microextraction	68:82	arg1	method					84:89	A simple and affordable microextraction method	44:89	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.	0:149	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	10	25	dep	99 	1598:1600	arg1	to					1595:1596	to	1595:1596	to	1595:1596	Satisfactory recoveries ranging from 87 to 99 % was achieved.					
36459855	9	26	theme	0.015-2 µg mL-1	1470:1484	arg1	range					1461:1465	the range	1457:1465	the range of 0.015-2 µg mL-1	1457:1484	The calibration curves were linear in the range of 0.015-2 µg mL-1 with a coefficient of determination (R2) in the range of 0.995-0.999.					
36459855	5	27	theme	green	934:938	arg1	GAC					962:964	GAC	962:964	GAC	962:964	The proposed method follows the principles of green analytical chemistry (GAC), as the unmodified filter paper used as the sorbent is inexpensive and biodegradable.					
36459855	5	27	theme	green	934:938	arg1	chemistry					951:959	green analytical chemistry	934:959	green analytical chemistry (GAC)	934:965	The proposed method follows the principles of green analytical chemistry (GAC), as the unmodified filter paper used as the sorbent is inexpensive and biodegradable.					
36459855	9	28	from	range	1534:1538	arg1	curves					1435:1440	The calibration curves	1419:1440	The calibration curves	1419:1440	The calibration curves were linear in the range of 0.015-2 µg mL-1 with a coefficient of determination (R2) in the range of 0.995-0.999.					
36459855	9	28	from	range	1534:1538	arg1	linear					1447:1452	linear	1447:1452	linear	1447:1452	The calibration curves were linear in the range of 0.015-2 µg mL-1 with a coefficient of determination (R2) in the range of 0.995-0.999.					
36459855	0	29	theme	Cellulose	0:8	arg1	CPSE					37:40	CPSE	37:40	CPSE	37:40	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	0	29	theme	Cellulose	0:8	arg1	extraction					25:34	Cellulose paper sorptive extraction	0:34	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.	0:149	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	4	30	used	used	830:833	arg2	optimization					734:745	Multivariate optimization	721:745	Multivariate optimization	721:745	Multivariate optimization, consisting of Placket-Burman design (PBD) and central composite design (CCD), was used to screen and optimize significant factors for CPSE.					
36459855	5	31	theme	proposed	892:899	arg1	method					901:906	The proposed method	888:906	The proposed method	888:906	The proposed method follows the principles of green analytical chemistry (GAC), as the unmodified filter paper used as the sorbent is inexpensive and biodegradable.					
36459855	1	32	dep	drugs	316:320	arg1	antidepressants					328:342	five antidepressants	323:342	five antidepressants	323:342	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	1	32	dep	drugs	316:320	arg1	benzodiazepines					350:364	four benzodiazepines	345:364	four benzodiazepines	345:364	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	1	32	dep	drugs	316:320	arg1	ketamine					371:378	ketamine	371:378	ketamine	371:378	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	4	33	theme	Multivariate	721:732	arg1	optimization					734:745	Multivariate optimization	721:745	Multivariate optimization	721:745	Multivariate optimization, consisting of Placket-Burman design (PBD) and central composite design (CCD), was used to screen and optimize significant factors for CPSE.					
36459855	0	34	theme	sorptive	16:23	arg1	CPSE					37:40	CPSE	37:40	CPSE	37:40	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	0	34	theme	sorptive	16:23	arg1	extraction					25:34	Cellulose paper sorptive extraction	0:34	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.	0:149	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	1	35	theme	filter	263:268	arg1	paper					270:274	unmodified laboratory filter paper	241:274	unmodified laboratory filter paper	241:274	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	7	36	theme	target	1239:1244	arg1	analytes					1246:1253	the target analytes	1235:1253	the target analytes	1235:1253	Under the optimized conditions, the limit of detection and quantification for the target analytes were estimated to be in the range of 0.003-0.035 and 0.010-0.117 µg mL-1, respectively.					
36459855	3	37	theme	diluted	622:628	arg1	samples					636:642	deproteinized diluted blood samples	608:642	deproteinized diluted blood samples	608:642	It involves adsorption of target analytes from deproteinized diluted blood samples on the unmodified cellulose paper followed by elution into 2 mL of methanol.					
36459855	4	38	theme	composite	802:810	arg1	CCD					820:822	CCD	820:822	CCD	820:822	Multivariate optimization, consisting of Placket-Burman design (PBD) and central composite design (CCD), was used to screen and optimize significant factors for CPSE.					
36459855	4	38	theme	composite	802:810	arg1	design					812:817	central composite design	794:817	central composite design (CCD)	794:823	Multivariate optimization, consisting of Placket-Burman design (PBD) and central composite design (CCD), was used to screen and optimize significant factors for CPSE.					
36459855	11	39	theme	potential	1733:1741	arg1	application					1743:1753	the potential application	1729:1753	the potential application of the proposed method	1729:1776	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	5	40	theme	chemistry	951:959	arg1	principles					920:929	the principles	916:929	the principles of green analytical chemistry (GAC)	916:965	The proposed method follows the principles of green analytical chemistry (GAC), as the unmodified filter paper used as the sorbent is inexpensive and biodegradable.					
36459855	0	41	from	drugs	113:117	arg1	blood					122:126	blood	122:126	blood	122:126	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	8	42	theme	relative	1360:1367	arg1	deviations					1378:1387	the relative standard deviations	1356:1387	the relative standard deviations	1356:1387	In contrast, the relative standard deviations were consistently below 10 %.					
36459855	0	43	theme	concept	142:148	arg1	proof					133:137	a proof	131:137	a proof of concept	131:148	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	11	44	theme	proposed	1762:1769	arg1	method					1771:1776	the proposed method	1758:1776	the proposed method	1758:1776	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	1	45	from	samples	398:404	arg1	drugs					316:320	nine basic drugs	305:320	nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples	305:404	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	1	45	from	samples	398:404	arg1	analysis					416:423	their analysis	410:423	their analysis by gas chromatography-mass spectrometry (GC-MS)	410:471	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	1	45	from	samples	398:404	arg1	extraction					291:300	extraction	291:300	extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples	291:404	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	3	46	theme	cellulose	662:670	arg1	paper					672:676	the unmodified cellulose paper	647:676	the unmodified cellulose paper followed by elution into 2 mL of methanol	647:718	It involves adsorption of target analytes from deproteinized diluted blood samples on the unmodified cellulose paper followed by elution into 2 mL of methanol.					
36459855	9	47	from	linear	1447:1452	arg1	range					1534:1538	the range	1530:1538	the range of 0.995-0.999	1530:1553	The calibration curves were linear in the range of 0.015-2 µg mL-1 with a coefficient of determination (R2) in the range of 0.995-0.999.					
36459855	9	47	from	linear	1447:1452	arg1	range					1461:1465	the range	1457:1465	the range of 0.015-2 µg mL-1	1457:1484	The calibration curves were linear in the range of 0.015-2 µg mL-1 with a coefficient of determination (R2) in the range of 0.995-0.999.					
36459855	0	48	from	blood	122:126	arg1	analysis					95:102	analysis	95:102	analysis of basic drugs in blood as a proof of concept	95:148	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	2	49	dep	paper	508:512	arg1	CPSE					535:538	CPSE	535:538	CPSE	535:538	The procedure termed as cellulose paper sorptive extraction (CPSE) is straightforward.					
36459855	2	49	dep	paper	508:512	arg1	extraction					523:532	sorptive extraction	514:532	cellulose paper sorptive extraction (CPSE)	498:539	The procedure termed as cellulose paper sorptive extraction (CPSE) is straightforward.					
36459855	6	50	theme	only	1099:1102	arg1	2 mL					1104:1107	only 2 mL	1099:1107	only 2 mL of MeOH	1099:1115	The technique is easy to perform and requires only 2 mL of MeOH during the entire extraction procedure.					
36459855	0	51	theme	basic	107:111	arg1	drugs					113:117	basic drugs	107:117	basic drugs in blood as a proof of concept	107:148	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	1	52	theme	chromatography-mass	432:450	arg1	GC-MS					466:470	GC-MS	466:470	GC-MS	466:470	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	1	52	theme	chromatography-mass	432:450	arg1	spectrometry					452:463	gas chromatography-mass spectrometry	428:463	gas chromatography-mass spectrometry (GC-MS)	428:471	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	3	53	theme	methanol	711:718	arg1	2 mL					703:706	2 mL	703:706	2 mL of methanol	703:718	It involves adsorption of target analytes from deproteinized diluted blood samples on the unmodified cellulose paper followed by elution into 2 mL of methanol.					
36459855	1	54	theme	sample	178:183	arg1	preparation					185:195	sample preparation	178:195	sample preparation procedure	178:205	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	11	55	from	samples	1674:1680	arg1	analysis					1642:1649	the analysis	1638:1649	the analysis of nine drugs in blood samples from the patients	1638:1698	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	11	55	from	samples	1674:1680	arg1	proof					1620:1624	proof	1620:1624	proof of concept	1620:1635	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	7	56	theme	0.010-0.117 µg mL-1	1308:1326	arg1	range					1283:1287	the range	1279:1287	the range of 0.003-0.035 and 0.010-0.117 µg mL-1, respectively	1279:1340	Under the optimized conditions, the limit of detection and quantification for the target analytes were estimated to be in the range of 0.003-0.035 and 0.010-0.117 µg mL-1, respectively.					
36459855	11	57	from	analysis	1642:1649	arg1	samples					1674:1680	blood samples	1668:1680	blood samples from the patients	1668:1698	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	0	58	from	analysis	95:102	arg1	blood					122:126	blood	122:126	blood	122:126	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	7	59	theme	0.003-0.035	1292:1302	arg1	range					1283:1287	the range	1279:1287	the range of 0.003-0.035 and 0.010-0.117 µg mL-1, respectively	1279:1340	Under the optimized conditions, the limit of detection and quantification for the target analytes were estimated to be in the range of 0.003-0.035 and 0.010-0.117 µg mL-1, respectively.					
36459855	5	60	theme	filter	986:991	arg1	paper					993:997	the unmodified filter paper	971:997	the unmodified filter paper used as the sorbent	971:1017	The proposed method follows the principles of green analytical chemistry (GAC), as the unmodified filter paper used as the sorbent is inexpensive and biodegradable.					
36459855	5	60	theme	filter	986:991	arg1	inexpensive					1022:1032	inexpensive	1022:1032	inexpensive	1022:1032	The proposed method follows the principles of green analytical chemistry (GAC), as the unmodified filter paper used as the sorbent is inexpensive and biodegradable.					
36459855	6	61	theme	entire	1128:1133	arg1	procedure					1146:1154	the entire extraction procedure	1124:1154	the entire extraction procedure	1124:1154	The technique is easy to perform and requires only 2 mL of MeOH during the entire extraction procedure.					
36459855	0	62	theme	simple	46:51	arg1	method					84:89	A simple and affordable microextraction method	44:89	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.	0:149	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	1	63	theme	human	386:390	arg1	samples					398:404	human blood samples	386:404	human blood samples	386:404	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	8	64	from	%	1416:1416	arg1	contrast					1346:1353	contrast	1346:1353	contrast	1346:1353	In contrast, the relative standard deviations were consistently below 10 %.					
36459855	0	65	theme	affordable	57:66	arg1	method					84:89	A simple and affordable microextraction method	44:89	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.	0:149	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	0	66	dep	extraction	25:34	arg1	method					84:89	A simple and affordable microextraction method	44:89	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.	0:149	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	1	67	theme	drugs	316:320	arg1	analysis					416:423	their analysis	410:423	their analysis by gas chromatography-mass spectrometry (GC-MS)	410:471	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	1	67	theme	drugs	316:320	arg1	extraction					291:300	extraction	291:300	extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples	291:404	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	11	68	from	drugs	1659:1663	arg1	patients					1691:1698	the patients	1687:1698	the patients	1687:1698	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	11	68	from	drugs	1659:1663	arg1	samples					1674:1680	blood samples	1668:1680	blood samples from the patients	1668:1698	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	0	69	theme	paper	10:14	arg1	CPSE					37:40	CPSE	37:40	CPSE	37:40	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	0	69	theme	paper	10:14	arg1	extraction					25:34	Cellulose paper sorptive extraction	0:34	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.	0:149	Cellulose paper sorptive extraction (CPSE): A simple and affordable microextraction method for analysis of basic drugs in blood as a proof of concept.					
36459855	10	70	theme	Satisfactory	1555:1566	arg1	recoveries					1568:1577	Satisfactory recoveries	1555:1577	Satisfactory recoveries ranging from 87 to 99 %	1555:1601	Satisfactory recoveries ranging from 87 to 99 % was achieved.					
36459855	1	71	theme	laboratory	252:261	arg1	paper					270:274	unmodified laboratory filter paper	241:274	unmodified laboratory filter paper	241:274	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	9	72	theme	determination	1508:1520	arg1	coefficient					1493:1503	a coefficient	1491:1503	a coefficient of determination (R2)	1491:1525	The calibration curves were linear in the range of 0.015-2 µg mL-1 with a coefficient of determination (R2) in the range of 0.995-0.999.					
36459855	3	73	theme	deproteinized	608:620	arg1	samples					636:642	deproteinized diluted blood samples	608:642	deproteinized diluted blood samples	608:642	It involves adsorption of target analytes from deproteinized diluted blood samples on the unmodified cellulose paper followed by elution into 2 mL of methanol.					
36459855	1	74	theme	paper	270:274	arg1	use					234:236	use	234:236	use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS)	234:471	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	7	75	theme	quantification	1216:1229	arg1	limit					1193:1197	the limit	1189:1197	the limit of detection and quantification for the target analytes	1189:1253	Under the optimized conditions, the limit of detection and quantification for the target analytes were estimated to be in the range of 0.003-0.035 and 0.010-0.117 µg mL-1, respectively.					
36459855	3	76	theme	analytes	594:601	arg1	adsorption					573:582	adsorption	573:582	adsorption of target analytes from deproteinized diluted blood samples	573:642	It involves adsorption of target analytes from deproteinized diluted blood samples on the unmodified cellulose paper followed by elution into 2 mL of methanol.					
36459855	7	77	theme	detection	1202:1210	arg1	limit					1193:1197	the limit	1189:1197	the limit of detection and quantification for the target analytes	1189:1253	Under the optimized conditions, the limit of detection and quantification for the target analytes were estimated to be in the range of 0.003-0.035 and 0.010-0.117 µg mL-1, respectively.					
36459855	11	78	theme	concept	1629:1635	arg1	analysis					1642:1649	the analysis	1638:1649	the analysis of nine drugs in blood samples from the patients	1638:1698	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	11	78	theme	concept	1629:1635	arg1	proof					1620:1624	proof	1620:1624	proof of concept	1620:1635	As proof of concept, the analysis of nine drugs in blood samples from the patients was performed to demonstrate the potential application of the proposed method.					
36459855	4	79	theme	central	794:800	arg1	CCD					820:822	CCD	820:822	CCD	820:822	Multivariate optimization, consisting of Placket-Burman design (PBD) and central composite design (CCD), was used to screen and optimize significant factors for CPSE.					
36459855	4	79	theme	central	794:800	arg1	design					812:817	central composite design	794:817	central composite design (CCD)	794:823	Multivariate optimization, consisting of Placket-Burman design (PBD) and central composite design (CCD), was used to screen and optimize significant factors for CPSE.					
36459855	5	80	theme	analytical	940:949	arg1	GAC					962:964	GAC	962:964	GAC	962:964	The proposed method follows the principles of green analytical chemistry (GAC), as the unmodified filter paper used as the sorbent is inexpensive and biodegradable.					
36459855	5	80	theme	analytical	940:949	arg1	chemistry					951:959	green analytical chemistry	934:959	green analytical chemistry (GAC)	934:965	The proposed method follows the principles of green analytical chemistry (GAC), as the unmodified filter paper used as the sorbent is inexpensive and biodegradable.					
36459855	1	81	from	analysis	416:423	arg1	samples					398:404	human blood samples	386:404	human blood samples	386:404	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36459855	3	82	theme	blood	630:634	arg1	samples					636:642	deproteinized diluted blood samples	608:642	deproteinized diluted blood samples	608:642	It involves adsorption of target analytes from deproteinized diluted blood samples on the unmodified cellulose paper followed by elution into 2 mL of methanol.					
36459855	4	83	theme	Placket-Burman	762:775	arg1	PBD					785:787	PBD	785:787	PBD	785:787	Multivariate optimization, consisting of Placket-Burman design (PBD) and central composite design (CCD), was used to screen and optimize significant factors for CPSE.					
36459855	4	83	theme	Placket-Burman	762:775	arg1	design					777:782	Placket-Burman design	762:782	Placket-Burman design (PBD)	762:788	Multivariate optimization, consisting of Placket-Burman design (PBD) and central composite design (CCD), was used to screen and optimize significant factors for CPSE.					
36459855	9	84	theme	0.995-0.999	1543:1553	arg1	range					1534:1538	the range	1530:1538	the range of 0.995-0.999	1530:1553	The calibration curves were linear in the range of 0.015-2 µg mL-1 with a coefficient of determination (R2) in the range of 0.995-0.999.					
36459855	3	85	from	samples	636:642	arg1	adsorption					573:582	adsorption	573:582	adsorption of target analytes from deproteinized diluted blood samples	573:642	It involves adsorption of target analytes from deproteinized diluted blood samples on the unmodified cellulose paper followed by elution into 2 mL of methanol.					
36459855	1	86	theme	present	212:218	arg1	work					220:223	the present work	208:223	the present work	208:223	Aiming towards simplifying sample preparation procedure, the present work explores use of unmodified laboratory filter paper as sorbent for extraction of nine basic drugs (five antidepressants, four benzodiazepines, and ketamine) from human blood samples and their analysis by gas chromatography-mass spectrometry (GC-MS).					
36568890	10	0	from	related	1749:1755	arg1	addition					1692:1699	addition	1692:1699	addition	1692:1699	In addition, the DEGGs encoding glycoconjugates were mainly related to extracellular matrix and axon guidance.					
36568890	6	1	theme	Siaα2-3	1162:1168	arg1	Gal/GalNAc					1170:1179	Siaα2-3 Gal/GalNAc	1162:1179	Siaα2-3 Gal/GalNAc	1162:1179	Lectin microarrays detected 14 significantly regulated lectins in VPA rats, with an up-regulation of high-mannose with antennary and down-regulation of Siaα2-3 Gal/GalNAc.					
36568890	7	2	theme	KEGG	1195:1198	arg1	resources					1209:1217	the KEGG and CAZy resources	1191:1217	the KEGG and CAZy resources	1191:1217	Based on the KEGG and CAZy resources, we assembled a comprehensive list of 961 glycan-related genes to focus our analysis on specific genes.					
36568890	9	3	theme	DEGGs	1482:1486	arg1	analysis					1470:1477	Functional analysis	1459:1477	Functional analysis of DEGGs encoding anabolic enzymes	1459:1512	Functional analysis of DEGGs encoding anabolic enzymes revealed that the process trimming to form core structure and glycan extension from core structure primarily changed, which is consistent with the changes in glycan patterns.					
36568890	9	4	from	structure	1603:1611	arg1	structure					1562:1570	core structure	1557:1570	core structure	1557:1570	Functional analysis of DEGGs encoding anabolic enzymes revealed that the process trimming to form core structure and glycan extension from core structure primarily changed, which is consistent with the changes in glycan patterns.					
36568890	9	4	from	structure	1603:1611	arg1	extension					1583:1591	glycan extension	1576:1591	glycan extension	1576:1591	Functional analysis of DEGGs encoding anabolic enzymes revealed that the process trimming to form core structure and glycan extension from core structure primarily changed, which is consistent with the changes in glycan patterns.					
36568890	4	5	theme	autism	631:636	arg1	model					622:626	an environmental factor-induced model	590:626	an environmental factor-induced model of autism	590:636	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	4	6	used	used	585:588	arg2	we					582:583	we	582:583	we	582:583	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	1	7	theme	repetitive	254:263	arg1	behaviors					265:273	repetitive behaviors	254:273	repetitive behaviors	254:273	Autism spectrum disorders (ASD) represent a group of neurodevelopmental defects characterized by social deficits and repetitive behaviors.					
36568890	4	8	theme	acid	698:701	arg1	injection					666:674	a single intraperitoneal injection	641:674	a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats	641:722	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	0	9	theme	Altered	0:6	arg1	expression					8:17	Altered expression	0:17	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.	0:135	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	10	10	from	addition	1692:1699	arg1	related					1749:1755	related	1749:1755	related	1749:1755	In addition, the DEGGs encoding glycoconjugates were mainly related to extracellular matrix and axon guidance.					
36568890	10	10	from	addition	1692:1699	arg1	DEGGs					1706:1710	the DEGGs	1702:1710	the DEGGs encoding glycoconjugates	1702:1735	In addition, the DEGGs encoding glycoconjugates were mainly related to extracellular matrix and axon guidance.					
36568890	5	11	theme	glycan	920:925	arg1	patterns					927:934	glycan patterns	920:934	glycan patterns	920:934	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	9	12	with	consistent	1641:1650	arg1	changes					1661:1667	the changes	1657:1667	the changes in glycan patterns	1657:1686	Functional analysis of DEGGs encoding anabolic enzymes revealed that the process trimming to form core structure and glycan extension from core structure primarily changed, which is consistent with the changes in glycan patterns.					
36568890	11	13	theme	aberrant	1872:1879	arg1	glycosylation					1881:1893	aberrant glycosylation	1872:1893	aberrant glycosylation	1872:1893	This study provides insights into the underlying molecular mechanism of aberrant glycosylation after prenatal VPA exposure, which may serve as potential biomarkers for the autism diagnosis.					
36568890	0	14	theme	acid	111:114	arg1	model					120:124	the valproic acid rat model	98:124	the valproic acid rat model of autism	98:134	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	5	15	from	confirmation	763:774	arg1	offspring					852:860	VPA-treated offspring	840:860	VPA-treated offspring	840:860	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	2	16	theme	Glycosylation	290:302	arg1	patterns					304:311	Glycosylation patterns	290:311	Glycosylation patterns	290:311	Alteration in Glycosylation patterns could influence the nervous system development and contribute to the molecular mechanism of ASD.					
36568890	6	17	theme	Lectin	1010:1015	arg1	microarrays					1017:1027	Lectin microarrays	1010:1027	Lectin microarrays	1010:1027	Lectin microarrays detected 14 significantly regulated lectins in VPA rats, with an up-regulation of high-mannose with antennary and down-regulation of Siaα2-3 Gal/GalNAc.					
36568890	11	18	theme	autism	1972:1977	arg1	diagnosis					1979:1987	the autism diagnosis	1968:1987	the autism diagnosis	1968:1987	This study provides insights into the underlying molecular mechanism of aberrant glycosylation after prenatal VPA exposure, which may serve as potential biomarkers for the autism diagnosis.					
36568890	4	19	theme	mg/kg	683:687	arg1	acid					698:701	400 mg/kg valproic acid	679:701	400 mg/kg valproic acid (VPA)	679:707	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	4	19	theme	mg/kg	683:687	arg1	VPA					704:706	VPA	704:706	VPA	704:706	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	0	20	theme	model	120:124	arg1	cortex					88:93	the medial prefrontal cortex	66:93	the medial prefrontal cortex of the valproic acid rat model of autism	66:134	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	11	21	theme	VPA	1910:1912	arg1	exposure					1914:1921	prenatal VPA exposure	1901:1921	prenatal VPA exposure	1901:1921	This study provides insights into the underlying molecular mechanism of aberrant glycosylation after prenatal VPA exposure, which may serve as potential biomarkers for the autism diagnosis.					
36568890	0	22	theme	autism	129:134	arg1	model					120:124	the valproic acid rat model	98:124	the valproic acid rat model of autism	98:134	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	5	23	theme	lectin	964:969	arg1	microarrays					971:981	lectin microarrays	964:981	lectin microarrays	964:981	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	7	24	theme	CAZy	1204:1207	arg1	resources					1209:1217	the KEGG and CAZy resources	1191:1217	the KEGG and CAZy resources	1191:1217	Based on the KEGG and CAZy resources, we assembled a comprehensive list of 961 glycan-related genes to focus our analysis on specific genes.					
36568890	4	25	theme	post-conception	736:750	arg1	day					727:729	day 12.5 post-conception	727:750	day 12.5 post-conception	727:750	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	9	26	theme	glycan	1576:1581	arg1	extension					1583:1591	glycan extension	1576:1591	glycan extension	1576:1591	Functional analysis of DEGGs encoding anabolic enzymes revealed that the process trimming to form core structure and glycan extension from core structure primarily changed, which is consistent with the changes in glycan patterns.					
36568890	5	27	theme	sociability	787:797	arg1	confirmation					763:774	confirmation	763:774	confirmation of reduced sociability	763:797	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	5	27	theme	sociability	787:797	arg1	behaviors					827:835	increased self-grooming behaviors	803:835	increased self-grooming behaviors in VPA-treated offspring	803:860	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	6	28	theme	VPA	1076:1078	arg1	rats					1080:1083	VPA rats	1076:1083	VPA rats	1076:1083	Lectin microarrays detected 14 significantly regulated lectins in VPA rats, with an up-regulation of high-mannose with antennary and down-regulation of Siaα2-3 Gal/GalNAc.					
36568890	0	29	theme	medial	70:75	arg1	cortex					88:93	the medial prefrontal cortex	66:93	the medial prefrontal cortex of the valproic acid rat model of autism	66:134	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	1	30	theme	spectrum	144:151	arg1	ASD					164:166	ASD	164:166	ASD	164:166	Autism spectrum disorders (ASD) represent a group of neurodevelopmental defects characterized by social deficits and repetitive behaviors.					
36568890	1	30	theme	spectrum	144:151	arg1	disorders					153:161	Autism spectrum disorders	137:161	Autism spectrum disorders (ASD)	137:167	Autism spectrum disorders (ASD) represent a group of neurodevelopmental defects characterized by social deficits and repetitive behaviors.					
36568890	8	31	theme	glycan-related	1409:1422	arg1	genes					1424:1428	glycan-related genes	1409:1428	107 differentially expressed glycan-related genes (DEGGs)	1380:1436	Of those, transcription results revealed that there were 107 differentially expressed glycan-related genes (DEGGs) after VPA treatment.					
36568890	8	31	theme	glycan-related	1409:1422	arg1	DEGGs					1431:1435	DEGGs	1431:1435	DEGGs	1431:1435	Of those, transcription results revealed that there were 107 differentially expressed glycan-related genes (DEGGs) after VPA treatment.					
36568890	11	32	theme	underlying	1838:1847	arg1	mechanism					1859:1867	the underlying molecular mechanism	1834:1867	the underlying molecular mechanism of aberrant glycosylation after prenatal VPA exposure	1834:1921	This study provides insights into the underlying molecular mechanism of aberrant glycosylation after prenatal VPA exposure, which may serve as potential biomarkers for the autism diagnosis.					
36568890	7	33	theme	glycan-related	1261:1274	arg1	genes					1276:1280	961 glycan-related genes	1257:1280	961 glycan-related genes	1257:1280	Based on the KEGG and CAZy resources, we assembled a comprehensive list of 961 glycan-related genes to focus our analysis on specific genes.					
36568890	3	34	with	Interaction	410:420	arg1	genes					464:468	susceptible genes	452:468	susceptible genes	452:468	Interaction of environmental factors with susceptible genes may affect expressions of glycosylation-related genes and thus result in abnormal glycosylation patterns.					
36568890	2	35	theme	ASD	405:407	arg1	mechanism					392:400	the molecular mechanism	378:400	the molecular mechanism of ASD	378:407	Alteration in Glycosylation patterns could influence the nervous system development and contribute to the molecular mechanism of ASD.					
36568890	9	36	theme	anabolic	1497:1504	arg1	enzymes					1506:1512	anabolic enzymes	1497:1512	anabolic enzymes	1497:1512	Functional analysis of DEGGs encoding anabolic enzymes revealed that the process trimming to form core structure and glycan extension from core structure primarily changed, which is consistent with the changes in glycan patterns.					
36568890	9	37	theme	core	1598:1601	arg1	structure					1603:1611	core structure	1598:1611	core structure primarily changed	1598:1629	Functional analysis of DEGGs encoding anabolic enzymes revealed that the process trimming to form core structure and glycan extension from core structure primarily changed, which is consistent with the changes in glycan patterns.					
36568890	3	38	theme	factors	439:445	arg1	Interaction					410:420	Interaction	410:420	Interaction of environmental factors with susceptible genes	410:468	Interaction of environmental factors with susceptible genes may affect expressions of glycosylation-related genes and thus result in abnormal glycosylation patterns.					
36568890	7	39	theme	comprehensive	1235:1247	arg1	list					1249:1252	a comprehensive list	1233:1252	a comprehensive list of 961 glycan-related genes	1233:1280	Based on the KEGG and CAZy resources, we assembled a comprehensive list of 961 glycan-related genes to focus our analysis on specific genes.					
36568890	8	40	theme	VPA	1444:1446	arg1	treatment					1448:1456	VPA treatment	1444:1456	VPA treatment	1444:1456	Of those, transcription results revealed that there were 107 differentially expressed glycan-related genes (DEGGs) after VPA treatment.					
36568890	9	41	from	changes	1661:1667	arg1	patterns					1679:1686	glycan patterns	1672:1686	glycan patterns	1672:1686	Functional analysis of DEGGs encoding anabolic enzymes revealed that the process trimming to form core structure and glycan extension from core structure primarily changed, which is consistent with the changes in glycan patterns.					
36568890	0	42	theme	patterns	29:36	arg1	expression					8:17	Altered expression	0:17	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.	0:135	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	3	43	theme	environmental	425:437	arg1	factors					439:445	environmental factors	425:445	environmental factors	425:445	Interaction of environmental factors with susceptible genes may affect expressions of glycosylation-related genes and thus result in abnormal glycosylation patterns.					
36568890	10	44	theme	axon	1785:1788	arg1	guidance					1790:1797	axon guidance	1785:1797	axon guidance	1785:1797	In addition, the DEGGs encoding glycoconjugates were mainly related to extracellular matrix and axon guidance.					
36568890	5	45	theme	VPA-treated	840:850	arg1	offspring					852:860	VPA-treated offspring	840:860	VPA-treated offspring	840:860	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	3	46	theme	glycosylation-related	496:516	arg1	genes					518:522	glycosylation-related genes	496:522	glycosylation-related genes	496:522	Interaction of environmental factors with susceptible genes may affect expressions of glycosylation-related genes and thus result in abnormal glycosylation patterns.					
36568890	1	47	theme	neurodevelopmental	190:207	arg1	defects					209:215	neurodevelopmental defects	190:215	neurodevelopmental defects characterized by social deficits and repetitive behaviors	190:273	Autism spectrum disorders (ASD) represent a group of neurodevelopmental defects characterized by social deficits and repetitive behaviors.					
36568890	6	48	with	high-mannose	1111:1122	arg1	antennary					1129:1137	antennary	1129:1137	antennary	1129:1137	Lectin microarrays detected 14 significantly regulated lectins in VPA rats, with an up-regulation of high-mannose with antennary and down-regulation of Siaα2-3 Gal/GalNAc.					
36568890	3	49	theme	abnormal	543:550	arg1	patterns					566:573	abnormal glycosylation patterns	543:573	abnormal glycosylation patterns	543:573	Interaction of environmental factors with susceptible genes may affect expressions of glycosylation-related genes and thus result in abnormal glycosylation patterns.					
36568890	9	50	theme	Functional	1459:1468	arg1	analysis					1470:1477	Functional analysis	1459:1477	Functional analysis of DEGGs encoding anabolic enzymes	1459:1512	Functional analysis of DEGGs encoding anabolic enzymes revealed that the process trimming to form core structure and glycan extension from core structure primarily changed, which is consistent with the changes in glycan patterns.					
36568890	6	51	theme	Gal/GalNAc	1170:1179	arg1	up-regulation					1094:1106	an up-regulation	1091:1106	an up-regulation of high-mannose with antennary	1091:1137	Lectin microarrays detected 14 significantly regulated lectins in VPA rats, with an up-regulation of high-mannose with antennary and down-regulation of Siaα2-3 Gal/GalNAc.					
36568890	6	51	theme	Gal/GalNAc	1170:1179	arg1	down-regulation					1143:1157	down-regulation	1143:1157	down-regulation of Siaα2-3 Gal/GalNAc	1143:1179	Lectin microarrays detected 14 significantly regulated lectins in VPA rats, with an up-regulation of high-mannose with antennary and down-regulation of Siaα2-3 Gal/GalNAc.					
36568890	4	52	theme	single	643:648	arg1	injection					666:674	a single intraperitoneal injection	641:674	a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats	641:722	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	7	53	theme	genes	1276:1280	arg1	list					1249:1252	a comprehensive list	1233:1252	a comprehensive list of 961 glycan-related genes	1233:1280	Based on the KEGG and CAZy resources, we assembled a comprehensive list of 961 glycan-related genes to focus our analysis on specific genes.					
36568890	1	54	theme	social	234:239	arg1	deficits					241:248	social deficits	234:248	social deficits	234:248	Autism spectrum disorders (ASD) represent a group of neurodevelopmental defects characterized by social deficits and repetitive behaviors.					
36568890	7	55	from	analysis	1295:1302	arg1	genes					1316:1320	specific genes	1307:1320	specific genes	1307:1320	Based on the KEGG and CAZy resources, we assembled a comprehensive list of 961 glycan-related genes to focus our analysis on specific genes.					
36568890	4	56	theme	factor-induced	607:620	arg1	model					622:626	an environmental factor-induced model	590:626	an environmental factor-induced model of autism	590:636	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	5	57	theme	glycan-related	940:953	arg1	genes					955:959	glycan-related genes	940:959	glycan-related genes	940:959	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	5	58	theme	expression	898:907	arg1	profile					909:915	the expression profile	894:915	the expression profile of glycan patterns and glycan-related genes	894:959	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	0	59	theme	glycan-related	42:55	arg1	genes					57:61	glycan-related genes	42:61	glycan-related genes	42:61	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	4	60	theme	valproic	689:696	arg1	acid					698:701	400 mg/kg valproic acid	679:701	400 mg/kg valproic acid (VPA)	679:707	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	4	60	theme	valproic	689:696	arg1	VPA					704:706	VPA	704:706	VPA	704:706	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	5	61	theme	increased	803:811	arg1	behaviors					827:835	increased self-grooming behaviors	803:835	increased self-grooming behaviors in VPA-treated offspring	803:860	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	11	62	theme	prenatal	1901:1908	arg1	exposure					1914:1921	prenatal VPA exposure	1901:1921	prenatal VPA exposure	1901:1921	This study provides insights into the underlying molecular mechanism of aberrant glycosylation after prenatal VPA exposure, which may serve as potential biomarkers for the autism diagnosis.					
36568890	0	63	theme	rat	116:118	arg1	model					120:124	the valproic acid rat model	98:124	the valproic acid rat model of autism	98:134	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	9	64	theme	process	1532:1538	arg1	trimming					1540:1547	the process trimming	1528:1547	the process trimming	1528:1547	Functional analysis of DEGGs encoding anabolic enzymes revealed that the process trimming to form core structure and glycan extension from core structure primarily changed, which is consistent with the changes in glycan patterns.					
36568890	5	65	theme	genes	955:959	arg1	profile					909:915	the expression profile	894:915	the expression profile of glycan patterns and glycan-related genes	894:959	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	11	66	theme	glycosylation	1881:1893	arg1	mechanism					1859:1867	the underlying molecular mechanism	1834:1867	the underlying molecular mechanism of aberrant glycosylation after prenatal VPA exposure	1834:1921	This study provides insights into the underlying molecular mechanism of aberrant glycosylation after prenatal VPA exposure, which may serve as potential biomarkers for the autism diagnosis.					
36568890	4	67	theme	400	679:681	arg1	mg/kg					683:687	mg/kg	683:687	mg/kg	683:687	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	9	68	theme	core	1557:1560	arg1	structure					1562:1570	core structure	1557:1570	core structure	1557:1570	Functional analysis of DEGGs encoding anabolic enzymes revealed that the process trimming to form core structure and glycan extension from core structure primarily changed, which is consistent with the changes in glycan patterns.					
36568890	8	69	theme	transcription	1333:1345	arg1	results					1347:1353	transcription results	1333:1353	transcription results	1333:1353	Of those, transcription results revealed that there were 107 differentially expressed glycan-related genes (DEGGs) after VPA treatment.					
36568890	5	70	from	alterations	879:889	arg1	profile					909:915	the expression profile	894:915	the expression profile of glycan patterns and glycan-related genes	894:959	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	2	71	theme	nervous	333:339	arg1	development					348:358	the nervous system development	329:358	the nervous system development	329:358	Alteration in Glycosylation patterns could influence the nervous system development and contribute to the molecular mechanism of ASD.					
36568890	5	72	theme	reduced	779:785	arg1	sociability					787:797	reduced sociability	779:797	reduced sociability	779:797	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	5	73	theme	patterns	927:934	arg1	profile					909:915	the expression profile	894:915	the expression profile of glycan patterns and glycan-related genes	894:959	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	2	74	from	Alteration	276:285	arg1	patterns					304:311	Glycosylation patterns	290:311	Glycosylation patterns	290:311	Alteration in Glycosylation patterns could influence the nervous system development and contribute to the molecular mechanism of ASD.					
36568890	0	75	theme	prefrontal	77:86	arg1	cortex					88:93	the medial prefrontal cortex	66:93	the medial prefrontal cortex of the valproic acid rat model of autism	66:134	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	1	76	theme	Autism	137:142	arg1	ASD					164:166	ASD	164:166	ASD	164:166	Autism spectrum disorders (ASD) represent a group of neurodevelopmental defects characterized by social deficits and repetitive behaviors.					
36568890	1	76	theme	Autism	137:142	arg1	disorders					153:161	Autism spectrum disorders	137:161	Autism spectrum disorders (ASD)	137:167	Autism spectrum disorders (ASD) represent a group of neurodevelopmental defects characterized by social deficits and repetitive behaviors.					
36568890	0	77	from	expression	8:17	arg1	cortex					88:93	the medial prefrontal cortex	66:93	the medial prefrontal cortex of the valproic acid rat model of autism	66:134	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	4	78	theme	female	712:717	arg1	rats					719:722	female rats	712:722	female rats	712:722	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	5	79	from	behaviors	827:835	arg1	offspring					852:860	VPA-treated offspring	840:860	VPA-treated offspring	840:860	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	6	80	theme	regulated	1055:1063	arg1	lectins					1065:1071	14 significantly regulated lectins	1038:1071	14 significantly regulated lectins in VPA rats	1038:1083	Lectin microarrays detected 14 significantly regulated lectins in VPA rats, with an up-regulation of high-mannose with antennary and down-regulation of Siaα2-3 Gal/GalNAc.					
36568890	8	81	theme	expressed	1399:1407	arg1	genes					1424:1428	glycan-related genes	1409:1428	107 differentially expressed glycan-related genes (DEGGs)	1380:1436	Of those, transcription results revealed that there were 107 differentially expressed glycan-related genes (DEGGs) after VPA treatment.					
36568890	8	81	theme	expressed	1399:1407	arg1	DEGGs					1431:1435	DEGGs	1431:1435	DEGGs	1431:1435	Of those, transcription results revealed that there were 107 differentially expressed glycan-related genes (DEGGs) after VPA treatment.					
36568890	10	82	theme	extracellular	1760:1772	arg1	matrix					1774:1779	extracellular matrix	1760:1779	extracellular matrix	1760:1779	In addition, the DEGGs encoding glycoconjugates were mainly related to extracellular matrix and axon guidance.					
36568890	0	83	theme	valproic	102:109	arg1	model					120:124	the valproic acid rat model	98:124	the valproic acid rat model of autism	98:134	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	11	84	theme	molecular	1849:1857	arg1	mechanism					1859:1867	the underlying molecular mechanism	1834:1867	the underlying molecular mechanism of aberrant glycosylation after prenatal VPA exposure	1834:1921	This study provides insights into the underlying molecular mechanism of aberrant glycosylation after prenatal VPA exposure, which may serve as potential biomarkers for the autism diagnosis.					
36568890	6	85	from	lectins	1065:1071	arg1	rats					1080:1083	VPA rats	1076:1083	VPA rats	1076:1083	Lectin microarrays detected 14 significantly regulated lectins in VPA rats, with an up-regulation of high-mannose with antennary and down-regulation of Siaα2-3 Gal/GalNAc.					
36568890	2	86	theme	molecular	382:390	arg1	mechanism					392:400	the molecular mechanism	378:400	the molecular mechanism of ASD	378:407	Alteration in Glycosylation patterns could influence the nervous system development and contribute to the molecular mechanism of ASD.					
36568890	0	87	theme	glycan	22:27	arg1	patterns					29:36	glycan patterns	22:36	glycan patterns	22:36	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	6	88	theme	high-mannose	1111:1122	arg1	up-regulation					1094:1106	an up-regulation	1091:1106	an up-regulation of high-mannose with antennary	1091:1137	Lectin microarrays detected 14 significantly regulated lectins in VPA rats, with an up-regulation of high-mannose with antennary and down-regulation of Siaα2-3 Gal/GalNAc.					
36568890	6	88	theme	high-mannose	1111:1122	arg1	down-regulation					1143:1157	down-regulation	1143:1157	down-regulation of Siaα2-3 Gal/GalNAc	1143:1179	Lectin microarrays detected 14 significantly regulated lectins in VPA rats, with an up-regulation of high-mannose with antennary and down-regulation of Siaα2-3 Gal/GalNAc.					
36568890	4	89	theme	environmental	593:605	arg1	model					622:626	an environmental factor-induced model	590:626	an environmental factor-induced model of autism	590:636	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	5	90	theme	self-grooming	813:825	arg1	behaviors					827:835	increased self-grooming behaviors	803:835	increased self-grooming behaviors in VPA-treated offspring	803:860	Following confirmation of reduced sociability and increased self-grooming behaviors in VPA-treated offspring, we analyzed the alterations in the expression profile of glycan patterns and glycan-related genes by lectin microarrays and RNA-seq, respectively.					
36568890	3	91	theme	susceptible	452:462	arg1	genes					464:468	susceptible genes	452:468	susceptible genes	452:468	Interaction of environmental factors with susceptible genes may affect expressions of glycosylation-related genes and thus result in abnormal glycosylation patterns.					
36568890	0	92	theme	genes	57:61	arg1	expression					8:17	Altered expression	0:17	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.	0:135	Altered expression of glycan patterns and glycan-related genes in the medial prefrontal cortex of the valproic acid rat model of autism.					
36568890	9	93	theme	glycan	1672:1677	arg1	patterns					1679:1686	glycan patterns	1672:1686	glycan patterns	1672:1686	Functional analysis of DEGGs encoding anabolic enzymes revealed that the process trimming to form core structure and glycan extension from core structure primarily changed, which is consistent with the changes in glycan patterns.					
36568890	2	94	theme	system	341:346	arg1	development					348:358	the nervous system development	329:358	the nervous system development	329:358	Alteration in Glycosylation patterns could influence the nervous system development and contribute to the molecular mechanism of ASD.					
36568890	11	95	theme	potential	1943:1951	arg1	insights					1820:1827	insights	1820:1827	insights	1820:1827	This study provides insights into the underlying molecular mechanism of aberrant glycosylation after prenatal VPA exposure, which may serve as potential biomarkers for the autism diagnosis.					
36568890	11	95	theme	potential	1943:1951	arg1	biomarkers					1953:1962	potential biomarkers	1943:1962	potential biomarkers for the autism diagnosis	1943:1987	This study provides insights into the underlying molecular mechanism of aberrant glycosylation after prenatal VPA exposure, which may serve as potential biomarkers for the autism diagnosis.					
36568890	3	96	theme	genes	518:522	arg1	expressions					481:491	expressions	481:491	expressions of glycosylation-related genes	481:522	Interaction of environmental factors with susceptible genes may affect expressions of glycosylation-related genes and thus result in abnormal glycosylation patterns.					
36568890	1	97	theme	defects	209:215	arg1	group					181:185	a group	179:185	a group of neurodevelopmental defects characterized by social deficits and repetitive behaviors	179:273	Autism spectrum disorders (ASD) represent a group of neurodevelopmental defects characterized by social deficits and repetitive behaviors.					
36568890	1	97	theme	defects	209:215	arg1	defects					209:215	neurodevelopmental defects	190:215	neurodevelopmental defects characterized by social deficits and repetitive behaviors	190:273	Autism spectrum disorders (ASD) represent a group of neurodevelopmental defects characterized by social deficits and repetitive behaviors.					
36568890	7	98	theme	specific	1307:1314	arg1	genes					1316:1320	specific genes	1307:1320	specific genes	1307:1320	Based on the KEGG and CAZy resources, we assembled a comprehensive list of 961 glycan-related genes to focus our analysis on specific genes.					
36568890	4	99	theme	intraperitoneal	650:664	arg1	injection					666:674	a single intraperitoneal injection	641:674	a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats	641:722	Here, we used an environmental factor-induced model of autism by a single intraperitoneal injection of 400 mg/kg valproic acid (VPA) to female rats at day 12.5 post-conception.					
36568890	3	100	theme	glycosylation	552:564	arg1	patterns					566:573	abnormal glycosylation patterns	543:573	abnormal glycosylation patterns	543:573	Interaction of environmental factors with susceptible genes may affect expressions of glycosylation-related genes and thus result in abnormal glycosylation patterns.					
36182825	2	0	theme	cellulose	500:508	arg1	surface					489:495	the hydrophilic 010 surface	469:495	the hydrophilic 010 surface of cellulose	469:508	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	7	1	theme	immediate	1495:1503	arg1	interface					1505:1513	the immediate interface	1491:1513	the immediate interface	1491:1513	This adsorption process is accompanied by increased exclusion of water between cellulose and graphene although some water localises between chains at the immediate interface.					
36182825	3	2	theme	infinite	885:892	arg1	sheet					894:898	an infinite sheet	882:898	an infinite sheet of pristine graphene	882:919	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	5	3	theme	global	1188:1193	arg1	untwisting					1195:1204	local and global untwisting	1178:1204	local and global untwisting of the microfibril	1178:1223	As cellulose chains adhere to the graphene surface, forming interactions via its CH and OH groups, we observe a degree of local and global untwisting of the microfibril.					
36182825	8	4	theme	graphene	1599:1606	arg1	surface					1608:1614	the graphene surface	1595:1614	the graphene surface	1595:1614	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	5	5	theme	OH	1144:1145	arg1	groups					1147:1152	its CH and OH groups	1133:1152	groups	1147:1152	As cellulose chains adhere to the graphene surface, forming interactions via its CH and OH groups, we observe a degree of local and global untwisting of the microfibril.					
36182825	5	6	theme	untwisting	1195:1204	arg1	degree					1168:1173	a degree	1166:1173	a degree of local and global untwisting of the microfibril	1166:1223	As cellulose chains adhere to the graphene surface, forming interactions via its CH and OH groups, we observe a degree of local and global untwisting of the microfibril.					
36182825	4	7	theme	microfibril	939:949	arg1	face					927:930	This face	922:930	This face of the microfibril	922:949	This face of the microfibril is of intermediate hydrophilicity and progressively associates with graphene over replicate simulations.					
36182825	6	8	with	interaction	1307:1317	arg1	microfibril					1328:1338	the microfibril	1324:1338	the microfibril	1324:1338	Complementary rippling of the graphene surface is also observed, as it adapts to interaction with the microfibril.					
36182825	2	9	theme	hydrogen	614:621	arg1	bonds					623:627	weakened hydrogen bonds	605:627	weakened hydrogen bonds between cellulose chains and partial permeation of water	605:684	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	2	10	theme	water	680:684	arg1	chains					647:652	cellulose chains	637:652	cellulose chains	637:652	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	2	10	theme	water	680:684	arg1	permeation					666:675	partial permeation	658:675	partial permeation of water	658:684	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	0	11	theme	amphiphilicity	81:94	arg1	Effects					70:76	Effects	70:76	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.	0:119	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.					
36182825	2	12	theme	weakened	605:612	arg1	bonds					623:627	weakened hydrogen bonds	605:627	weakened hydrogen bonds between cellulose chains and partial permeation of water	605:684	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	3	13	theme	pristine	903:910	arg1	graphene					912:919	pristine graphene	903:919	pristine graphene	903:919	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	7	14	theme	water	1406:1410	arg1	exclusion					1393:1401	increased exclusion	1383:1401	increased exclusion of water between cellulose and graphene	1383:1441	This adsorption process is accompanied by increased exclusion of water between cellulose and graphene although some water localises between chains at the immediate interface.					
36182825	3	15	theme	aqueous	746:752	arg1	solution					754:761	aqueous solution	746:761	aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues,	746:875	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	2	16	theme	partial	658:664	arg1	permeation					666:675	partial permeation	658:675	partial permeation of water	658:684	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	8	17	theme	interactions	1719:1730	arg1	types					1710:1714	the types	1706:1714	the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials	1706:1809	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	8	17	theme	interactions	1719:1730	arg1	nature					1682:1687	the amphiphilic nature	1666:1687	the amphiphilic nature of cellulose	1666:1700	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	2	18	theme	aqueous	397:403	arg1	simulations					433:443	aqueous solution molecular dynamics simulations	397:443	aqueous solution molecular dynamics simulations	397:443	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	2	19	theme	molecular	414:422	arg1	simulations					433:443	aqueous solution molecular dynamics simulations	397:443	aqueous solution molecular dynamics simulations	397:443	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	0	20	theme	molecular	99:107	arg1	simulation					109:118	molecular simulation	99:118	molecular simulation	99:118	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.					
36182825	8	21	theme	cellulose	1546:1554	arg1	microfibril					1556:1566	a cellulose microfibril	1544:1566	a cellulose microfibril	1544:1566	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	0	22	theme	pristine	42:49	arg1	graphene					51:58	pristine graphene	42:58	pristine graphene	42:58	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.					
36182825	3	23	from	interaction	731:741	arg1	solution					754:761	aqueous solution	746:761	aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues,	746:875	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	5	24	theme	microfibril	1213:1223	arg1	untwisting					1195:1204	local and global untwisting	1178:1204	local and global untwisting of the microfibril	1178:1223	As cellulose chains adhere to the graphene surface, forming interactions via its CH and OH groups, we observe a degree of local and global untwisting of the microfibril.					
36182825	2	25	theme	solution	405:412	arg1	simulations					433:443	aqueous solution molecular dynamics simulations	397:443	aqueous solution molecular dynamics simulations	397:443	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	0	26	theme	Iβ	10:11	arg1	interaction					25:35	Cellulose Iβ microfibril interaction	0:35	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.	0:119	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.					
36182825	4	27	dep	simulations	1043:1053	arg1	replicate					1033:1041	replicate	1033:1041	replicate	1033:1041	This face of the microfibril is of intermediate hydrophilicity and progressively associates with graphene over replicate simulations.					
36182825	7	28	from	interface	1505:1513	arg1	chains					1481:1486	chains	1481:1486	chains at the immediate interface	1481:1513	This adsorption process is accompanied by increased exclusion of water between cellulose and graphene although some water localises between chains at the immediate interface.					
36182825	2	29	theme	hydrophobic	316:326	arg1	surface					328:334	the model 100 hydrophobic surface	302:334	the model 100 hydrophobic surface of cellulose	302:347	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	1	30	theme	new	203:205	arg1	materials					207:215	new materials	203:215	new materials	203:215	Graphene-cellulose interactions have considerable potential in the development of new materials.					
36182825	0	31	theme	Cellulose	0:8	arg1	interaction					25:35	Cellulose Iβ microfibril interaction	0:35	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.	0:119	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.					
36182825	6	32	theme	surface	1265:1271	arg1	rippling					1240:1247	Complementary rippling	1226:1247	Complementary rippling of the graphene surface	1226:1271	Complementary rippling of the graphene surface is also observed, as it adapts to interaction with the microfibril.					
36182825	2	33	theme	cellulose	637:645	arg1	chains					647:652	cellulose chains	637:652	cellulose chains	637:652	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	2	34	from	graphene	385:392	arg1	simulations					433:443	aqueous solution molecular dynamics simulations	397:443	aqueous solution molecular dynamics simulations	397:443	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	5	35	theme	cellulose	1059:1067	arg1	chains					1069:1074	cellulose chains	1059:1074	cellulose chains	1059:1074	As cellulose chains adhere to the graphene surface, forming interactions via its CH and OH groups, we observe a degree of local and global untwisting of the microfibril.					
36182825	1	36	theme	materials	207:215	arg1	development					188:198	the development	184:198	the development of new materials	184:215	Graphene-cellulose interactions have considerable potential in the development of new materials.					
36182825	3	37	theme	amphiphilic	770:780	arg1	surface					786:792	the amphiphilic 110 surface	766:792	the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues,	766:875	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	2	38	theme	model	306:310	arg1	surface					328:334	the model 100 hydrophobic surface	302:334	the model 100 hydrophobic surface of cellulose	302:347	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	0	39	theme	microfibril	13:23	arg1	interaction					25:35	Cellulose Iβ microfibril interaction	0:35	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.	0:119	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.					
36182825	5	40	theme	local	1178:1182	arg1	untwisting					1195:1204	local and global untwisting	1178:1204	local and global untwisting of the microfibril	1178:1223	As cellulose chains adhere to the graphene surface, forming interactions via its CH and OH groups, we observe a degree of local and global untwisting of the microfibril.					
36182825	8	41	theme	new	1764:1766	arg1	materials					1801:1809	new graphene-carbohydrate biopolymer materials	1764:1809	new graphene-carbohydrate biopolymer materials	1764:1809	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	2	42	dep	work	244:247	arg1	Biomacromolecules2016					250:270	Biomacromolecules2016	250:270	Biomacromolecules2016	250:270	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	2	42	dep	work	244:247	arg1	1771					277:280	1771	277:280	1771	277:280	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	2	43	theme	surface	489:495	arg1	model					460:464	a model	458:464	a model of the hydrophilic 010 surface of cellulose	458:508	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	2	44	theme	cellulose	339:347	arg1	surface					328:334	the model 100 hydrophobic surface	302:334	the model 100 hydrophobic surface of cellulose	302:347	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	2	45	theme	hydrophobic	564:574	arg1	face					576:579	a more hydrophobic face	557:579	a more hydrophobic face	557:579	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	5	46	theme	graphene	1090:1097	arg1	surface					1099:1105	the graphene surface	1086:1105	the graphene surface	1086:1105	As cellulose chains adhere to the graphene surface, forming interactions via its CH and OH groups, we observe a degree of local and global untwisting of the microfibril.					
36182825	2	47	theme	010	485:487	arg1	surface					489:495	the hydrophilic 010 surface	469:495	the hydrophilic 010 surface of cellulose	469:508	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	3	48	theme	graphene	912:919	arg1	sheet					894:898	an infinite sheet	882:898	an infinite sheet of pristine graphene	882:919	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	0	49	with	interaction	25:35	arg1	graphene					51:58	pristine graphene	42:58	pristine graphene	42:58	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.					
36182825	2	50	theme	dynamics	424:431	arg1	simulations					433:443	aqueous solution molecular dynamics simulations	397:443	aqueous solution molecular dynamics simulations	397:443	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	2	51	theme	hydrophilic	473:483	arg1	surface					489:495	the hydrophilic 010 surface	469:495	the hydrophilic 010 surface of cellulose	469:508	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	7	52	theme	increased	1383:1391	arg1	exclusion					1393:1401	increased exclusion	1383:1401	increased exclusion of water between cellulose and graphene	1383:1441	This adsorption process is accompanied by increased exclusion of water between cellulose and graphene although some water localises between chains at the immediate interface.					
36182825	8	53	theme	graphene-carbohydrate	1768:1788	arg1	materials					1801:1809	new graphene-carbohydrate biopolymer materials	1764:1809	new graphene-carbohydrate biopolymer materials	1764:1809	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	8	54	theme	amphiphilic	1670:1680	arg1	nature					1682:1687	the amphiphilic nature	1666:1687	the amphiphilic nature of cellulose	1666:1700	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	1	55	contain	have	153:156	arg1	interactions					140:151	Graphene-cellulose interactions	121:151	Graphene-cellulose interactions	121:151	Graphene-cellulose interactions have considerable potential in the development of new materials.					
36182825	1	55	contain	have	153:156	arg2	potential					171:179	considerable potential	158:179	considerable potential	158:179	Graphene-cellulose interactions have considerable potential in the development of new materials.					
36182825	2	56	theme	computational	230:242	arg1	work					244:247	previous computational work	221:247	previous computational work (Biomacromolecules2016, 16, 1771)	221:281	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	6	57	theme	graphene	1256:1263	arg1	surface					1265:1271	the graphene surface	1252:1271	the graphene surface	1252:1271	Complementary rippling of the graphene surface is also observed, as it adapts to interaction with the microfibril.					
36182825	1	58	theme	Graphene-cellulose	121:138	arg1	interactions					140:151	Graphene-cellulose interactions	121:151	Graphene-cellulose interactions	121:151	Graphene-cellulose interactions have considerable potential in the development of new materials.					
36182825	3	59	theme	glucosyl	858:865	arg1	residues					867:874	40 glucosyl residues	855:874	40 glucosyl residues	855:874	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	3	60	theme	surface	786:792	arg1	solution					754:761	aqueous solution	746:761	aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues,	746:875	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	8	61	theme	biopolymer	1790:1799	arg1	materials					1801:1809	new graphene-carbohydrate biopolymer materials	1764:1809	new graphene-carbohydrate biopolymer materials	1764:1809	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	2	62	theme	previous	221:228	arg1	work					244:247	previous computational work	221:247	previous computational work (Biomacromolecules2016, 16, 1771)	221:281	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	0	63	dep	interaction	25:35	arg1	Effects					70:76	Effects	70:76	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.	0:119	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.					
36182825	6	64	theme	Complementary	1226:1238	arg1	rippling					1240:1247	Complementary rippling	1226:1247	Complementary rippling of the graphene surface	1226:1271	Complementary rippling of the graphene surface is also observed, as it adapts to interaction with the microfibril.					
36182825	3	65	theme	residues	867:874	arg1	chains					845:850	36 chains	842:850	36 chains of 40 glucosyl residues	842:874	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	3	66	theme	microfibril	812:822	arg1	model					824:828	a cellulose Iβ microfibril model	797:828	a cellulose Iβ microfibril model	797:828	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	7	67	theme	adsorption	1346:1355	arg1	process					1357:1363	This adsorption process	1341:1363	This adsorption process	1341:1363	This adsorption process is accompanied by increased exclusion of water between cellulose and graphene although some water localises between chains at the immediate interface.					
36182825	8	68	theme	cellulose	1692:1700	arg1	types					1710:1714	the types	1706:1714	the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials	1706:1809	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	8	68	theme	cellulose	1692:1700	arg1	nature					1682:1687	the amphiphilic nature	1666:1687	the amphiphilic nature of cellulose	1666:1700	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	3	69	theme	model	824:828	arg1	surface					786:792	the amphiphilic 110 surface	766:792	the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues,	766:875	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	2	70	theme	progressive	520:530	arg1	rearrangement					532:544	progressive rearrangement	520:544	progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water	520:684	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	8	71	theme	structural	1629:1638	arg1	accommodation					1640:1652	mutual structural accommodation	1622:1652	mutual structural accommodation	1622:1652	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	8	72	theme	microfibril	1556:1566	arg1	propensity					1530:1539	The predicted propensity	1516:1539	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface	1516:1614	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	1	73	theme	considerable	158:169	arg1	potential					171:179	considerable potential	158:179	considerable potential	158:179	Graphene-cellulose interactions have considerable potential in the development of new materials.					
36182825	3	74	with	interaction	731:741	arg1	sheet					894:898	an infinite sheet	882:898	an infinite sheet of pristine graphene	882:919	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	3	75	theme	cellulose	799:807	arg1	model					824:828	a cellulose Iβ microfibril model	797:828	a cellulose Iβ microfibril model	797:828	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
36182825	4	76	theme	intermediate	957:968	arg1	hydrophilicity					970:983	intermediate hydrophilicity	957:983	intermediate hydrophilicity	957:983	This face of the microfibril is of intermediate hydrophilicity and progressively associates with graphene over replicate simulations.					
36182825	0	77	from	interaction	25:35	arg1	water					63:67	water	63:67	water	63:67	Cellulose Iβ microfibril interaction with pristine graphene in water: Effects of amphiphilicity by molecular simulation.					
36182825	8	78	theme	mutual	1622:1627	arg1	accommodation					1640:1652	mutual structural accommodation	1622:1652	mutual structural accommodation	1622:1652	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	2	79	theme	pristine	376:383	arg1	graphene					385:392	pristine graphene	376:392	pristine graphene in aqueous solution molecular dynamics simulations	376:443	In previous computational work (Biomacromolecules2016, 16, 1771), we predicted that the model 100 hydrophobic surface of cellulose interacted favourably with pristine graphene in aqueous solution molecular dynamics simulations; conversely, a model of the hydrophilic 010 surface of cellulose exhibited progressive rearrangement to present a more hydrophobic face with the graphene, with weakened hydrogen bonds between cellulose chains and partial permeation of water.					
36182825	8	80	theme	predicted	1520:1528	arg1	propensity					1530:1539	The predicted propensity	1516:1539	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface	1516:1614	The predicted propensity of a cellulose microfibril to adsorb spontaneously on the graphene surface, with mutual structural accommodation, highlights the amphiphilic nature of cellulose and the types of interactions that can be harnessed to design new graphene-carbohydrate biopolymer materials.					
36182825	3	81	theme	Iβ	809:810	arg1	model					824:828	a cellulose Iβ microfibril model	797:828	a cellulose Iβ microfibril model	797:828	Here, we extend this work by simulating the interaction in aqueous solution of the amphiphilic 110 surface of a cellulose Iβ microfibril model, comprising 36 chains of 40 glucosyl residues, with an infinite sheet of pristine graphene.					
35500766	4	0	theme	ultrafast	614:622	arg1	polymerization					624:637	an ultrafast polymerization	611:637	an ultrafast polymerization	611:637	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	0	1	theme	ultrafast	82:90	arg1	polymerization					92:105	ultrafast polymerization	82:105	ultrafast polymerization	82:105	A self-healing and self-adhesive chitosan based ion-conducting hydrogel sensor by ultrafast polymerization.					
35500766	6	2	theme	application	1011:1021	arg1	range					1023:1027	their application range	1005:1027	their application range	1005:1027	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	5	3	theme	subtle	809:814	arg1	motions					821:827	major and subtle body motions	799:827	major and subtle body motions	799:827	The hydrogels are ion conductive and show good sensing performance in detecting major and subtle body motions.					
35500766	6	4	theme	CS/TA-Fe	894:901	arg1	hydrogels					903:911	the PAM@CS/TA-Fe hydrogels	886:911	the PAM@CS/TA-Fe hydrogels	886:911	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	2	5	theme	efficient	301:309	arg1	preparation					279:289	the better preparation	268:289	the better preparation with more efficient	268:309	And the better preparation with more efficient is always common aspiration.					
35500766	2	5	theme	efficient	301:309	arg1	aspiration					328:337	common aspiration	321:337	common aspiration	321:337	And the better preparation with more efficient is always common aspiration.					
35500766	6	6	theme	electronic	1054:1063	arg1	equipment					1065:1073	wearable and flexible electronic equipment	1032:1073	wearable and flexible electronic equipment	1032:1073	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	6	7	theme	@	893:893	arg1	hydrogels					903:911	the PAM@CS/TA-Fe hydrogels	886:911	the PAM@CS/TA-Fe hydrogels	886:911	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	6	8	theme	PAM	890:892	arg1	hydrogels					903:911	the PAM@CS/TA-Fe hydrogels	886:911	the PAM@CS/TA-Fe hydrogels	886:911	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	6	9	theme	flexible	1045:1052	arg1	equipment					1065:1073	wearable and flexible electronic equipment	1032:1073	wearable and flexible electronic equipment	1032:1073	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	6	10	theme	good	958:961	arg1	property					976:983	good self-healing property	958:983	good self-healing property	958:983	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	1	11	theme	human	172:176	arg1	monitoring					187:196	human activity monitoring	172:196	human activity monitoring	172:196	Recently, owing to the wide applications in electronic skin and human activity monitoring, flexible hydrogel strain sensors have attracted great attention.					
35500766	6	12	theme	wearable	1032:1039	arg1	equipment					1065:1073	wearable and flexible electronic equipment	1032:1073	wearable and flexible electronic equipment	1032:1073	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	6	13	theme	excellent	923:931	arg1	performance					942:952	excellent adhesion performance	923:952	excellent adhesion performance	923:952	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	1	14	theme	activity	178:185	arg1	monitoring					187:196	human activity monitoring	172:196	human activity monitoring	172:196	Recently, owing to the wide applications in electronic skin and human activity monitoring, flexible hydrogel strain sensors have attracted great attention.					
35500766	0	15	theme	self-healing	2:13	arg1	chitosan					33:40	A self-healing and self-adhesive chitosan	0:40	A self-healing and self-adhesive chitosan	0:40	A self-healing and self-adhesive chitosan based ion-conducting hydrogel sensor by ultrafast polymerization.					
35500766	6	16	theme	noncovalent	867:877	arg1	bonds					879:883	the multiple dynamic noncovalent bonds	846:883	the multiple dynamic noncovalent bonds	846:883	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	1	17	from	applications	136:147	arg1	monitoring					187:196	human activity monitoring	172:196	human activity monitoring	172:196	Recently, owing to the wide applications in electronic skin and human activity monitoring, flexible hydrogel strain sensors have attracted great attention.					
35500766	1	17	from	applications	136:147	arg1	skin					163:166	electronic skin	152:166	electronic skin	152:166	Recently, owing to the wide applications in electronic skin and human activity monitoring, flexible hydrogel strain sensors have attracted great attention.					
35500766	4	18	theme	hydrogel	658:665	arg1	CS/TA-Fe					671:678	the composite hydrogel PAM@CS/TA-Fe	644:678	the composite hydrogel PAM@CS/TA-Fe	644:678	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	4	19	theme	polyphenol	500:509	arg1	TA					524:525	TA	524:525	TA	524:525	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	4	19	theme	polyphenol	500:509	arg1	acid					518:521	plant polyphenol tannic acid	494:521	plant polyphenol tannic acid (TA)	494:526	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	2	20	theme	common	321:326	arg1	preparation					279:289	the better preparation	268:289	the better preparation with more efficient	268:309	And the better preparation with more efficient is always common aspiration.					
35500766	2	20	theme	common	321:326	arg1	aspiration					328:337	common aspiration	321:337	common aspiration	321:337	And the better preparation with more efficient is always common aspiration.					
35500766	4	21	theme	redox	588:592	arg1	Fe3+					560:563	Fe3+	560:563	Fe3+	560:563	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	4	21	theme	redox	588:592	arg1	agent					594:598	redox agent	588:598	redox agent	588:598	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	0	22	theme	self-adhesive	19:31	arg1	chitosan					33:40	A self-healing and self-adhesive chitosan	0:40	A self-healing and self-adhesive chitosan	0:40	A self-healing and self-adhesive chitosan based ion-conducting hydrogel sensor by ultrafast polymerization.					
35500766	4	23	theme	plant	494:498	arg1	TA					524:525	TA	524:525	TA	524:525	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	4	23	theme	plant	494:498	arg1	acid					518:521	plant polyphenol tannic acid	494:521	plant polyphenol tannic acid (TA)	494:526	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	4	24	theme	@	670:670	arg1	CS/TA-Fe					671:678	the composite hydrogel PAM@CS/TA-Fe	644:678	the composite hydrogel PAM@CS/TA-Fe	644:678	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	1	25	theme	flexible	199:206	arg1	sensors					224:230	flexible hydrogel strain sensors	199:230	flexible hydrogel strain sensors	199:230	Recently, owing to the wide applications in electronic skin and human activity monitoring, flexible hydrogel strain sensors have attracted great attention.					
35500766	4	26	theme	composite	648:656	arg1	CS/TA-Fe					671:678	the composite hydrogel PAM@CS/TA-Fe	644:678	the composite hydrogel PAM@CS/TA-Fe	644:678	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	4	27	theme	PAM	667:669	arg1	CS/TA-Fe					671:678	the composite hydrogel PAM@CS/TA-Fe	644:678	the composite hydrogel PAM@CS/TA-Fe	644:678	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	1	28	theme	hydrogel	208:215	arg1	sensors					224:230	flexible hydrogel strain sensors	199:230	flexible hydrogel strain sensors	199:230	Recently, owing to the wide applications in electronic skin and human activity monitoring, flexible hydrogel strain sensors have attracted great attention.					
35500766	3	29	theme	chitosan	397:404	arg1	matrix					411:416	chitosan (CS) matrix	397:416	chitosan (CS) matrix	397:416	In this work, acrylamide (AM) was in situ polymerized in chitosan (CS) matrix to prepare hydrogels (PAM@CS).					
35500766	4	30	theme	tannic	511:516	arg1	TA					524:525	TA	524:525	TA	524:525	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	4	30	theme	tannic	511:516	arg1	acid					518:521	plant polyphenol tannic acid	494:521	plant polyphenol tannic acid (TA)	494:526	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	1	31	theme	strain	217:222	arg1	sensors					224:230	flexible hydrogel strain sensors	199:230	flexible hydrogel strain sensors	199:230	Recently, owing to the wide applications in electronic skin and human activity monitoring, flexible hydrogel strain sensors have attracted great attention.					
35500766	5	32	theme	good	761:764	arg1	performance					774:784	good sensing performance	761:784	good sensing performance	761:784	The hydrogels are ion conductive and show good sensing performance in detecting major and subtle body motions.					
35500766	4	33	theme	natural	477:483	arg1	mussels					485:491	natural mussels	477:491	natural mussels	477:491	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	0	34	theme	hydrogel	63:70	arg1	sensor					72:77	ion-conducting hydrogel sensor	48:77	ion-conducting hydrogel sensor	48:77	A self-healing and self-adhesive chitosan based ion-conducting hydrogel sensor by ultrafast polymerization.					
35500766	6	35	theme	multiple	850:857	arg1	bonds					879:883	the multiple dynamic noncovalent bonds	846:883	the multiple dynamic noncovalent bonds	846:883	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	5	36	theme	body	816:819	arg1	motions					821:827	major and subtle body motions	799:827	major and subtle body motions	799:827	The hydrogels are ion conductive and show good sensing performance in detecting major and subtle body motions.					
35500766	0	37	theme	ion-conducting	48:61	arg1	sensor					72:77	ion-conducting hydrogel sensor	48:77	ion-conducting hydrogel sensor	48:77	A self-healing and self-adhesive chitosan based ion-conducting hydrogel sensor by ultrafast polymerization.					
35500766	4	38	theme	mussels	485:491	arg1	adhesion					465:472	the adhesion	461:472	the adhesion of natural mussels	461:491	Inspired by the adhesion of natural mussels, plant polyphenol tannic acid (TA) was introduced into the system, Fe3+ was also introduced as redox agent to perform an ultrafast polymerization, and the composite hydrogel PAM@CS/TA-Fe can be prepared at 60 °C within 1 min.					
35500766	6	39	theme	dynamic	859:865	arg1	bonds					879:883	the multiple dynamic noncovalent bonds	846:883	the multiple dynamic noncovalent bonds	846:883	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	2	40	theme	better	272:277	arg1	preparation					279:289	the better preparation	268:289	the better preparation with more efficient	268:309	And the better preparation with more efficient is always common aspiration.					
35500766	2	40	theme	better	272:277	arg1	aspiration					328:337	common aspiration	321:337	common aspiration	321:337	And the better preparation with more efficient is always common aspiration.					
35500766	1	41	theme	wide	131:134	arg1	applications					136:147	the wide applications	127:147	the wide applications in electronic skin and human activity monitoring	127:196	Recently, owing to the wide applications in electronic skin and human activity monitoring, flexible hydrogel strain sensors have attracted great attention.					
35500766	6	42	theme	adhesion	933:940	arg1	performance					942:952	excellent adhesion performance	923:952	excellent adhesion performance	923:952	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	5	43	theme	major	799:803	arg1	motions					821:827	major and subtle body motions	799:827	major and subtle body motions	799:827	The hydrogels are ion conductive and show good sensing performance in detecting major and subtle body motions.					
35500766	3	44	theme	@	443:443	arg1	CS					444:445	@CS	443:445	PAM@CS	440:445	In this work, acrylamide (AM) was in situ polymerized in chitosan (CS) matrix to prepare hydrogels (PAM@CS).					
35500766	3	45	from	work	348:351	arg1	polymerized					382:392	polymerized	382:392	polymerized	382:392	In this work, acrylamide (AM) was in situ polymerized in chitosan (CS) matrix to prepare hydrogels (PAM@CS).					
35500766	6	46	theme	self-healing	963:974	arg1	property					976:983	good self-healing property	958:983	good self-healing property	958:983	Benefiting from the multiple dynamic noncovalent bonds, the PAM@CS/TA-Fe hydrogels also show excellent adhesion performance and good self-healing property, which would expand their application range in wearable and flexible electronic equipment.					
35500766	5	47	theme	sensing	766:772	arg1	performance					774:784	good sensing performance	761:784	good sensing performance	761:784	The hydrogels are ion conductive and show good sensing performance in detecting major and subtle body motions.					
35500766	1	48	theme	great	247:251	arg1	attention					253:261	great attention	247:261	great attention	247:261	Recently, owing to the wide applications in electronic skin and human activity monitoring, flexible hydrogel strain sensors have attracted great attention.					
35500766	3	49	from	polymerized	382:392	arg1	matrix					411:416	chitosan (CS) matrix	397:416	chitosan (CS) matrix	397:416	In this work, acrylamide (AM) was in situ polymerized in chitosan (CS) matrix to prepare hydrogels (PAM@CS).					
35500766	3	49	from	polymerized	382:392	arg1	work					348:351	this work	343:351	this work	343:351	In this work, acrylamide (AM) was in situ polymerized in chitosan (CS) matrix to prepare hydrogels (PAM@CS).					
35500766	3	50	dep	PAM	440:442	arg1	CS					444:445	@CS	443:445	PAM@CS	440:445	In this work, acrylamide (AM) was in situ polymerized in chitosan (CS) matrix to prepare hydrogels (PAM@CS).					
35500766	3	51	from	matrix	411:416	arg1	polymerized					382:392	polymerized	382:392	polymerized	382:392	In this work, acrylamide (AM) was in situ polymerized in chitosan (CS) matrix to prepare hydrogels (PAM@CS).					
35500766	1	52	theme	electronic	152:161	arg1	skin					163:166	electronic skin	152:166	electronic skin	152:166	Recently, owing to the wide applications in electronic skin and human activity monitoring, flexible hydrogel strain sensors have attracted great attention.					
36280858	10	0	link	compost-derived	1570:1584	arg1	microbiomes					1586:1596	compost-derived microbiomes	1570:1596	compost-derived microbiomes	1570:1596	CONCLUSIONS The two-tier cultivation of compost-derived microbiomes on sorghum led to the establishment of microbiomes for which community structure and performance could be assessed.					
36280858	5	1	theme	microbiome	731:740	arg1	performance					742:752	comparative microbiome performance	719:752	comparative microbiome performance	719:752	RESULTS To establish a system assessing comparative microbiome performance, parallel microbiomes were cultivated on sorghum (Sorghum bicolor L. Moench) from compost inocula.					
36280858	10	2	theme	microbiomes	1586:1596	arg1	cultivation					1555:1565	The two-tier cultivation	1542:1565	The two-tier cultivation of compost-derived microbiomes on sorghum led to the establishment of microbiomes for which community structure and performance could be assessed	1542:1711	CONCLUSIONS The two-tier cultivation of compost-derived microbiomes on sorghum led to the establishment of microbiomes for which community structure and performance could be assessed.					
36280858	7	3	theme	gene	1001:1004	arg1	dynamics					1017:1024	gene expression dynamics	1001:1024	gene expression dynamics	1001:1024	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	4	4	theme	synthetic	646:654	arg1	communities					666:676	specialized synthetic microbial communities	634:676	specialized synthetic microbial communities	634:676	These microbiomes provide a unique resource to identify key interacting functional microbial groups and to guide the design of specialized synthetic microbial communities.					
36280858	4	5	theme	functional	579:588	arg1	groups					600:605	key interacting functional microbial groups	563:605	key interacting functional microbial groups	563:605	These microbiomes provide a unique resource to identify key interacting functional microbial groups and to guide the design of specialized synthetic microbial communities.					
36280858	7	6	theme	adapted	1086:1092	arg1	communities					1112:1122	the adapted sorghum-degrading communities	1082:1122	the adapted sorghum-degrading communities	1082:1122	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	8	7	attach	linked	1296:1301	arg2	stages					1280:1285	successive stages	1269:1285	successive stages that are linked to enzymes that deconstruct plant cell wall polymers	1269:1354	Functional analysis demonstrated that the microbiomes proceeded through successive stages that are linked to enzymes that deconstruct plant cell wall polymers.					
36280858	8	7	attach	linked	1296:1301	arg1	enzymes					1306:1312	enzymes	1306:1312	enzymes that deconstruct plant cell wall polymers	1306:1354	Functional analysis demonstrated that the microbiomes proceeded through successive stages that are linked to enzymes that deconstruct plant cell wall polymers.					
36280858	7	8	theme	Network	972:978	arg1	reconstructions					980:994	Network reconstructions	972:994	Network reconstructions from gene expression dynamics	972:1024	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	4	9	theme	specialized	634:644	arg1	communities					666:676	specialized synthetic microbial communities	634:676	specialized synthetic microbial communities	634:676	These microbiomes provide a unique resource to identify key interacting functional microbial groups and to guide the design of specialized synthetic microbial communities.					
36280858	3	10	theme	down	409:412	arg1	walls					419:423	down cell walls	409:423	down cell walls	409:423	Although most understanding of biological cell wall deconstruction has been obtained from isolates, cultivated microbiomes that break down cell walls have emerged as new sources for biotechnologically relevant microbes and enzymes.					
36280858	4	11	theme	interacting	567:577	arg1	groups					600:605	key interacting functional microbial groups	563:605	key interacting functional microbial groups	563:605	These microbiomes provide a unique resource to identify key interacting functional microbial groups and to guide the design of specialized synthetic microbial communities.					
36280858	8	12	theme	Functional	1197:1206	arg1	analysis					1208:1215	Functional analysis	1197:1215	Functional analysis	1197:1215	Functional analysis demonstrated that the microbiomes proceeded through successive stages that are linked to enzymes that deconstruct plant cell wall polymers.					
36280858	7	13	theme	sorghum-degrading	1094:1110	arg1	communities					1112:1122	the adapted sorghum-degrading communities	1082:1122	the adapted sorghum-degrading communities	1082:1122	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	11	14	theme	subtle	1755:1760	arg1	differences					1762:1772	subtle differences	1755:1772	subtle differences in community composition	1755:1797	The work reinforces the observation that subtle differences in community composition and the genomic content of strains may lead to significant differences in community performance.					
36280858	3	15	theme	new	441:443	arg1	sources					445:451	new sources	441:451	new sources for biotechnologically relevant microbes and enzymes	441:504	Although most understanding of biological cell wall deconstruction has been obtained from isolates, cultivated microbiomes that break down cell walls have emerged as new sources for biotechnologically relevant microbes and enzymes.					
36280858	3	16	theme	cell	414:417	arg1	walls					419:423	down cell walls	409:423	down cell walls	409:423	Although most understanding of biological cell wall deconstruction has been obtained from isolates, cultivated microbiomes that break down cell walls have emerged as new sources for biotechnologically relevant microbes and enzymes.					
36280858	4	17	theme	unique	535:540	arg1	resource					542:549	a unique resource	533:549	a unique resource to identify key interacting functional microbial groups and to guide the design of specialized synthetic microbial communities	533:676	These microbiomes provide a unique resource to identify key interacting functional microbial groups and to guide the design of specialized synthetic microbial communities.					
36280858	5	18	theme	parallel	755:762	arg1	microbiomes					764:774	parallel microbiomes	755:774	parallel microbiomes	755:774	RESULTS To establish a system assessing comparative microbiome performance, parallel microbiomes were cultivated on sorghum (Sorghum bicolor L. Moench) from compost inocula.					
36280858	11	19	theme	community	1777:1785	arg1	composition					1787:1797	community composition	1777:1797	community composition	1777:1797	The work reinforces the observation that subtle differences in community composition and the genomic content of strains may lead to significant differences in community performance.					
36280858	4	20	theme	microbial	656:664	arg1	communities					666:676	specialized synthetic microbial communities	634:676	specialized synthetic microbial communities	634:676	These microbiomes provide a unique resource to identify key interacting functional microbial groups and to guide the design of specialized synthetic microbial communities.					
36280858	2	21	theme	Native	179:184	arg1	microbiomes					198:208	Native and adapted microbiomes	179:208	Native and adapted microbiomes	179:208	Native and adapted microbiomes can be particularly effective at plant cell wall deconstruction.					
36280858	11	22	theme	genomic	1807:1813	arg1	content					1815:1821	the genomic content	1803:1821	the genomic content of strains	1803:1832	The work reinforces the observation that subtle differences in community composition and the genomic content of strains may lead to significant differences in community performance.					
36280858	11	23	from	differences	1762:1772	arg1	composition					1787:1797	community composition	1777:1797	community composition	1777:1797	The work reinforces the observation that subtle differences in community composition and the genomic content of strains may lead to significant differences in community performance.					
36280858	10	24	from	cultivation	1555:1565	arg1	sorghum					1601:1607	sorghum	1601:1607	sorghum led to the establishment of microbiomes for which community structure and performance could be assessed	1601:1711	CONCLUSIONS The two-tier cultivation of compost-derived microbiomes on sorghum led to the establishment of microbiomes for which community structure and performance could be assessed.					
36280858	8	25	theme	wall	1342:1345	arg1	polymers					1347:1354	plant cell wall polymers	1331:1354	plant cell wall polymers	1331:1354	Functional analysis demonstrated that the microbiomes proceeded through successive stages that are linked to enzymes that deconstruct plant cell wall polymers.					
36280858	6	26	theme	biochemical	870:880	arg1	assays					882:887	biochemical assays	870:887	biochemical assays	870:887	Biomass loss and biochemical assays indicated that these microbiomes diverged in their ability to deconstruct biomass.					
36280858	7	27	theme	potential	1052:1060	arg1	Filomicrobium					1148:1160	Filomicrobium	1148:1160	Filomicrobium	1148:1160	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	7	27	theme	potential	1052:1060	arg1	Gemmatimonadetes					1167:1182	Gemmatimonadetes	1167:1182	Gemmatimonadetes	1167:1182	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	7	27	theme	potential	1052:1060	arg1	interactions					1062:1073	potential interactions	1052:1073	potential interactions	1052:1073	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	7	27	theme	potential	1052:1060	arg1	Actinotalea					1135:1145	Actinotalea	1135:1145	Actinotalea	1135:1145	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	1	28	theme	BACKGROUND	96:105	arg1	walls					118:122	BACKGROUND Plant cell walls	96:122	BACKGROUND Plant cell walls	96:122	BACKGROUND Plant cell walls are interwoven structures recalcitrant to degradation.					
36280858	1	28	theme	BACKGROUND	96:105	arg1	structures					139:148	interwoven structures	128:148	interwoven structures recalcitrant to degradation	128:176	BACKGROUND Plant cell walls are interwoven structures recalcitrant to degradation.					
36280858	9	29	theme	network	1376:1382	arg1	combination					1361:1371	The combination	1357:1371	The combination of network and functional analysis	1357:1406	The combination of network and functional analysis highlighted the importance of cellulose-degrading Actinobacteria in differentiating the performance of these microbiomes.					
36280858	10	30	theme	community	1659:1667	arg1	structure					1669:1677	community structure	1659:1677	community structure	1659:1677	CONCLUSIONS The two-tier cultivation of compost-derived microbiomes on sorghum led to the establishment of microbiomes for which community structure and performance could be assessed.					
36280858	5	31	theme	compost	836:842	arg1	inocula					844:850	compost inocula	836:850	compost inocula	836:850	RESULTS To establish a system assessing comparative microbiome performance, parallel microbiomes were cultivated on sorghum (Sorghum bicolor L. Moench) from compost inocula.					
36280858	1	32	theme	Plant	107:111	arg1	walls					118:122	BACKGROUND Plant cell walls	96:122	BACKGROUND Plant cell walls	96:122	BACKGROUND Plant cell walls are interwoven structures recalcitrant to degradation.					
36280858	1	32	theme	Plant	107:111	arg1	structures					139:148	interwoven structures	128:148	interwoven structures recalcitrant to degradation	128:176	BACKGROUND Plant cell walls are interwoven structures recalcitrant to degradation.					
36280858	2	33	from	deconstruction	259:272	arg1	effective					230:238	effective	230:238	effective	230:238	Native and adapted microbiomes can be particularly effective at plant cell wall deconstruction.					
36280858	3	34	theme	relevant	476:483	arg1	microbes					485:492	microbes	485:492	microbes	485:492	Although most understanding of biological cell wall deconstruction has been obtained from isolates, cultivated microbiomes that break down cell walls have emerged as new sources for biotechnologically relevant microbes and enzymes.					
36280858	0	35	theme	Low-abundance	0:12	arg1	populations					14:24	Low-abundance populations	0:24	Low-abundance populations	0:24	Low-abundance populations distinguish microbiome performance in plant cell wall deconstruction.					
36280858	5	36	theme	Sorghum	804:810	arg1	bicolor					812:818	Sorghum bicolor	804:818	Sorghum bicolor L. Moench	804:828	RESULTS To establish a system assessing comparative microbiome performance, parallel microbiomes were cultivated on sorghum (Sorghum bicolor L. Moench) from compost inocula.					
36280858	3	37	theme	most	284:287	arg1	understanding					289:301	most understanding	284:301	most understanding of biological cell wall deconstruction	284:340	Although most understanding of biological cell wall deconstruction has been obtained from isolates, cultivated microbiomes that break down cell walls have emerged as new sources for biotechnologically relevant microbes and enzymes.					
36280858	9	38	theme	functional	1388:1397	arg1	analysis					1399:1406	functional analysis	1388:1406	functional analysis	1388:1406	The combination of network and functional analysis highlighted the importance of cellulose-degrading Actinobacteria in differentiating the performance of these microbiomes.					
36280858	1	39	theme	cell	113:116	arg1	walls					118:122	BACKGROUND Plant cell walls	96:122	BACKGROUND Plant cell walls	96:122	BACKGROUND Plant cell walls are interwoven structures recalcitrant to degradation.					
36280858	1	39	theme	cell	113:116	arg1	structures					139:148	interwoven structures	128:148	interwoven structures recalcitrant to degradation	128:176	BACKGROUND Plant cell walls are interwoven structures recalcitrant to degradation.					
36280858	5	40	theme	L.	820:821	arg1	Moench					823:828	Sorghum bicolor L. Moench	804:828	Sorghum bicolor L. Moench	804:828	RESULTS To establish a system assessing comparative microbiome performance, parallel microbiomes were cultivated on sorghum (Sorghum bicolor L. Moench) from compost inocula.					
36280858	5	40	theme	L.	820:821	arg1	sorghum					795:801	sorghum	795:801	sorghum (Sorghum bicolor L. Moench) from compost inocula	795:850	RESULTS To establish a system assessing comparative microbiome performance, parallel microbiomes were cultivated on sorghum (Sorghum bicolor L. Moench) from compost inocula.					
36280858	0	41	theme	microbiome	38:47	arg1	performance					49:59	microbiome performance	38:59	microbiome performance	38:59	Low-abundance populations distinguish microbiome performance in plant cell wall deconstruction.					
36280858	4	42	dep	resource	542:549	arg1	identify					554:561	identify	554:561	to identify key interacting functional microbial groups	551:605	These microbiomes provide a unique resource to identify key interacting functional microbial groups and to guide the design of specialized synthetic microbial communities.					
36280858	4	42	dep	resource	542:549	arg1	guide					614:618	guide	614:618	to guide the design of specialized synthetic microbial communities	611:676	These microbiomes provide a unique resource to identify key interacting functional microbial groups and to guide the design of specialized synthetic microbial communities.					
36280858	3	43	theme	wall	322:325	arg1	deconstruction					327:340	biological cell wall deconstruction	306:340	biological cell wall deconstruction	306:340	Although most understanding of biological cell wall deconstruction has been obtained from isolates, cultivated microbiomes that break down cell walls have emerged as new sources for biotechnologically relevant microbes and enzymes.					
36280858	9	44	theme	analysis	1399:1406	arg1	combination					1361:1371	The combination	1357:1371	The combination of network and functional analysis	1357:1406	The combination of network and functional analysis highlighted the importance of cellulose-degrading Actinobacteria in differentiating the performance of these microbiomes.					
36280858	7	45	from	dynamics	1017:1024	arg1	reconstructions					980:994	Network reconstructions	972:994	Network reconstructions from gene expression dynamics	972:1024	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	3	46	theme	deconstruction	327:340	arg1	understanding					289:301	most understanding	284:301	most understanding of biological cell wall deconstruction	284:340	Although most understanding of biological cell wall deconstruction has been obtained from isolates, cultivated microbiomes that break down cell walls have emerged as new sources for biotechnologically relevant microbes and enzymes.					
36280858	11	47	theme	strains	1826:1832	arg1	content					1815:1821	the genomic content	1803:1821	the genomic content of strains	1803:1832	The work reinforces the observation that subtle differences in community composition and the genomic content of strains may lead to significant differences in community performance.					
36280858	11	47	theme	strains	1826:1832	arg1	differences					1762:1772	subtle differences	1755:1772	subtle differences in community composition	1755:1797	The work reinforces the observation that subtle differences in community composition and the genomic content of strains may lead to significant differences in community performance.					
36280858	11	48	from	content	1815:1821	arg1	composition					1787:1797	community composition	1777:1797	community composition	1777:1797	The work reinforces the observation that subtle differences in community composition and the genomic content of strains may lead to significant differences in community performance.					
36280858	3	49	theme	biological	306:315	arg1	deconstruction					327:340	biological cell wall deconstruction	306:340	biological cell wall deconstruction	306:340	Although most understanding of biological cell wall deconstruction has been obtained from isolates, cultivated microbiomes that break down cell walls have emerged as new sources for biotechnologically relevant microbes and enzymes.					
36280858	5	50	theme	bicolor	812:818	arg1	Moench					823:828	Sorghum bicolor L. Moench	804:828	Sorghum bicolor L. Moench	804:828	RESULTS To establish a system assessing comparative microbiome performance, parallel microbiomes were cultivated on sorghum (Sorghum bicolor L. Moench) from compost inocula.					
36280858	5	50	theme	bicolor	812:818	arg1	sorghum					795:801	sorghum	795:801	sorghum (Sorghum bicolor L. Moench) from compost inocula	795:850	RESULTS To establish a system assessing comparative microbiome performance, parallel microbiomes were cultivated on sorghum (Sorghum bicolor L. Moench) from compost inocula.					
36280858	7	51	dep	Actinotalea	1135:1145	arg1	populations					1184:1194	populations	1184:1194	populations	1184:1194	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	11	52	from	differences	1858:1868	arg1	performance					1883:1893	community performance	1873:1893	community performance	1873:1893	The work reinforces the observation that subtle differences in community composition and the genomic content of strains may lead to significant differences in community performance.					
36280858	3	53	theme	cell	317:320	arg1	deconstruction					327:340	biological cell wall deconstruction	306:340	biological cell wall deconstruction	306:340	Although most understanding of biological cell wall deconstruction has been obtained from isolates, cultivated microbiomes that break down cell walls have emerged as new sources for biotechnologically relevant microbes and enzymes.					
36280858	6	54	theme	Biomass	853:859	arg1	loss					861:864	Biomass loss	853:864	Biomass loss	853:864	Biomass loss and biochemical assays indicated that these microbiomes diverged in their ability to deconstruct biomass.					
36280858	0	55	theme	cell	70:73	arg1	deconstruction					80:93	plant cell wall deconstruction	64:93	plant cell wall deconstruction	64:93	Low-abundance populations distinguish microbiome performance in plant cell wall deconstruction.					
36280858	4	56	theme	microbial	590:598	arg1	groups					600:605	key interacting functional microbial groups	563:605	key interacting functional microbial groups	563:605	These microbiomes provide a unique resource to identify key interacting functional microbial groups and to guide the design of specialized synthetic microbial communities.					
36280858	2	57	theme	adapted	190:196	arg1	microbiomes					198:208	Native and adapted microbiomes	179:208	Native and adapted microbiomes	179:208	Native and adapted microbiomes can be particularly effective at plant cell wall deconstruction.					
36280858	2	58	theme	wall	254:257	arg1	deconstruction					259:272	plant cell wall deconstruction	243:272	plant cell wall deconstruction	243:272	Native and adapted microbiomes can be particularly effective at plant cell wall deconstruction.					
36280858	11	59	theme	significant	1846:1856	arg1	differences					1858:1868	significant differences	1846:1868	significant differences in community performance	1846:1893	The work reinforces the observation that subtle differences in community composition and the genomic content of strains may lead to significant differences in community performance.					
36280858	1	60	theme	interwoven	128:137	arg1	walls					118:122	BACKGROUND Plant cell walls	96:122	BACKGROUND Plant cell walls	96:122	BACKGROUND Plant cell walls are interwoven structures recalcitrant to degradation.					
36280858	1	60	theme	interwoven	128:137	arg1	structures					139:148	interwoven structures	128:148	interwoven structures recalcitrant to degradation	128:176	BACKGROUND Plant cell walls are interwoven structures recalcitrant to degradation.					
36280858	0	61	theme	plant	64:68	arg1	deconstruction					80:93	plant cell wall deconstruction	64:93	plant cell wall deconstruction	64:93	Low-abundance populations distinguish microbiome performance in plant cell wall deconstruction.					
36280858	9	62	theme	cellulose-degrading	1438:1456	arg1	Actinobacteria					1458:1471	cellulose-degrading Actinobacteria	1438:1471	cellulose-degrading Actinobacteria	1438:1471	The combination of network and functional analysis highlighted the importance of cellulose-degrading Actinobacteria in differentiating the performance of these microbiomes.					
36280858	2	63	theme	plant	243:247	arg1	deconstruction					259:272	plant cell wall deconstruction	243:272	plant cell wall deconstruction	243:272	Native and adapted microbiomes can be particularly effective at plant cell wall deconstruction.					
36280858	2	64	theme	cell	249:252	arg1	deconstruction					259:272	plant cell wall deconstruction	243:272	plant cell wall deconstruction	243:272	Native and adapted microbiomes can be particularly effective at plant cell wall deconstruction.					
36280858	5	65	theme	comparative	719:729	arg1	performance					742:752	comparative microbiome performance	719:752	comparative microbiome performance	719:752	RESULTS To establish a system assessing comparative microbiome performance, parallel microbiomes were cultivated on sorghum (Sorghum bicolor L. Moench) from compost inocula.					
36280858	12	66	dep	Abstract	1902:1909	arg1	Video					1896:1900	Video	1896:1900	Video	1896:1900	Video Abstract.					
36280858	10	67	theme	microbiomes	1637:1647	arg1	establishment					1620:1632	the establishment	1616:1632	the establishment of microbiomes for which community structure and performance could be assessed	1616:1711	CONCLUSIONS The two-tier cultivation of compost-derived microbiomes on sorghum led to the establishment of microbiomes for which community structure and performance could be assessed.					
36280858	8	68	theme	successive	1269:1278	arg1	stages					1280:1285	successive stages	1269:1285	successive stages that are linked to enzymes that deconstruct plant cell wall polymers	1269:1354	Functional analysis demonstrated that the microbiomes proceeded through successive stages that are linked to enzymes that deconstruct plant cell wall polymers.					
36280858	9	69	theme	Actinobacteria	1458:1471	arg1	importance					1424:1433	the importance	1420:1433	the importance of cellulose-degrading Actinobacteria in differentiating the performance of these microbiomes	1420:1527	The combination of network and functional analysis highlighted the importance of cellulose-degrading Actinobacteria in differentiating the performance of these microbiomes.					
36280858	10	70	theme	two-tier	1546:1553	arg1	cultivation					1555:1565	The two-tier cultivation	1542:1565	The two-tier cultivation of compost-derived microbiomes on sorghum led to the establishment of microbiomes for which community structure and performance could be assessed	1542:1711	CONCLUSIONS The two-tier cultivation of compost-derived microbiomes on sorghum led to the establishment of microbiomes for which community structure and performance could be assessed.					
36280858	1	71	theme	recalcitrant	150:161	arg1	walls					118:122	BACKGROUND Plant cell walls	96:122	BACKGROUND Plant cell walls	96:122	BACKGROUND Plant cell walls are interwoven structures recalcitrant to degradation.					
36280858	1	71	theme	recalcitrant	150:161	arg1	structures					139:148	interwoven structures	128:148	interwoven structures recalcitrant to degradation	128:176	BACKGROUND Plant cell walls are interwoven structures recalcitrant to degradation.					
36280858	7	72	theme	key	1037:1039	arg1	Filomicrobium					1148:1160	Filomicrobium	1148:1160	Filomicrobium	1148:1160	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	7	72	theme	key	1037:1039	arg1	groups					1041:1046	key groups	1037:1046	key groups	1037:1046	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	7	72	theme	key	1037:1039	arg1	Actinotalea					1135:1145	Actinotalea	1135:1145	Actinotalea	1135:1145	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	7	72	theme	key	1037:1039	arg1	Gemmatimonadetes					1167:1182	Gemmatimonadetes	1167:1182	Gemmatimonadetes	1167:1182	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	8	73	theme	cell	1337:1340	arg1	polymers					1347:1354	plant cell wall polymers	1331:1354	plant cell wall polymers	1331:1354	Functional analysis demonstrated that the microbiomes proceeded through successive stages that are linked to enzymes that deconstruct plant cell wall polymers.					
36280858	10	74	theme	compost-derived	1570:1584	arg1	microbiomes					1586:1596	compost-derived microbiomes	1570:1596	compost-derived microbiomes	1570:1596	CONCLUSIONS The two-tier cultivation of compost-derived microbiomes on sorghum led to the establishment of microbiomes for which community structure and performance could be assessed.					
36280858	11	75	theme	community	1873:1881	arg1	performance					1883:1893	community performance	1873:1893	community performance	1873:1893	The work reinforces the observation that subtle differences in community composition and the genomic content of strains may lead to significant differences in community performance.					
36280858	7	76	theme	expression	1006:1015	arg1	dynamics					1017:1024	gene expression dynamics	1001:1024	gene expression dynamics	1001:1024	Network reconstructions from gene expression dynamics identified key groups and potential interactions within the adapted sorghum-degrading communities, including Actinotalea, Filomicrobium, and Gemmatimonadetes populations.					
36280858	3	77	theme	cultivated	375:384	arg1	microbiomes					386:396	cultivated microbiomes	375:396	cultivated microbiomes that break down cell walls	375:423	Although most understanding of biological cell wall deconstruction has been obtained from isolates, cultivated microbiomes that break down cell walls have emerged as new sources for biotechnologically relevant microbes and enzymes.					
36280858	5	78	from	inocula	844:850	arg1	Moench					823:828	Sorghum bicolor L. Moench	804:828	Sorghum bicolor L. Moench	804:828	RESULTS To establish a system assessing comparative microbiome performance, parallel microbiomes were cultivated on sorghum (Sorghum bicolor L. Moench) from compost inocula.					
36280858	5	78	from	inocula	844:850	arg1	sorghum					795:801	sorghum	795:801	sorghum (Sorghum bicolor L. Moench) from compost inocula	795:850	RESULTS To establish a system assessing comparative microbiome performance, parallel microbiomes were cultivated on sorghum (Sorghum bicolor L. Moench) from compost inocula.					
36280858	0	79	theme	wall	75:78	arg1	deconstruction					80:93	plant cell wall deconstruction	64:93	plant cell wall deconstruction	64:93	Low-abundance populations distinguish microbiome performance in plant cell wall deconstruction.					
36280858	4	80	theme	key	563:565	arg1	groups					600:605	key interacting functional microbial groups	563:605	key interacting functional microbial groups	563:605	These microbiomes provide a unique resource to identify key interacting functional microbial groups and to guide the design of specialized synthetic microbial communities.					
36280858	9	81	theme	microbiomes	1517:1527	arg1	performance					1496:1506	the performance	1492:1506	the performance of these microbiomes	1492:1527	The combination of network and functional analysis highlighted the importance of cellulose-degrading Actinobacteria in differentiating the performance of these microbiomes.					
36280858	4	82	theme	communities	666:676	arg1	design					624:629	the design	620:629	the design of specialized synthetic microbial communities	620:676	These microbiomes provide a unique resource to identify key interacting functional microbial groups and to guide the design of specialized synthetic microbial communities.					
36280858	8	83	theme	plant	1331:1335	arg1	polymers					1347:1354	plant cell wall polymers	1331:1354	plant cell wall polymers	1331:1354	Functional analysis demonstrated that the microbiomes proceeded through successive stages that are linked to enzymes that deconstruct plant cell wall polymers.					
36736174	3	0	theme	drug	470:473	arg1	system					484:489	drug delivery system	470:489	drug delivery system	470:489	Therefore, anti-adhesive PP mesh was designed with poly(vinyl alcohol) (PVA) hydrogel and liposomes drug delivery system.					
36736174	7	1	dep	In	943:944	arg1	vitro					946:950	vitro	946:950	vitro	946:950	In vitro cell tests revealed the excellent cytocompatibility and the potential to inhibit cell adhesion of the modified PP mesh.					
36736174	11	2	theme	significant	1554:1564	arg1	tissue					1581:1586	significant looser fibrous tissue	1554:1586	significant looser fibrous tissue	1554:1586	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	1	3	theme	mechanical	260:269	arg1	properties					271:280	mechanical properties	260:280	mechanical properties	260:280	Polypropylene (PP) mesh has been widely used in hernia repair as prosthesis material owing to its excellent balanced biocompatibility and mechanical properties.					
36736174	12	4	theme	current	1650:1656	arg1	findings					1658:1665	The current findings	1646:1665	The current findings	1646:1665	The current findings manifested that this type of RPM@LPS/PVA10-c-PP might be a potential candidate for anti-adhesion treatment.					
36736174	10	5	theme	adhesion	1313:1320	arg1	score					1322:1326	the adhesion score	1309:1326	the adhesion score of RPM@LPS/PVA10-c-PP mesh	1309:1353	At 30 days, the adhesion score of RPM@LPS/PVA10-c-PP mesh was 1.37 ± 0.75, however the original PP was 3 ± 0.71.					
36736174	10	5	theme	adhesion	1313:1320	arg1	1.37 ± 0.75					1359:1369	1.37 ± 0.75	1359:1369	1.37 ± 0.75	1359:1369	At 30 days, the adhesion score of RPM@LPS/PVA10-c-PP mesh was 1.37 ± 0.75, however the original PP was 3 ± 0.71.					
36736174	8	6	theme	anti-adhesive	1086:1098	arg1	effects					1100:1106	the anti-adhesive effects	1082:1106	the anti-adhesive effects of the RPM@LPS/PVA10-c-PP mesh	1082:1137	Moreover, the anti-adhesive effects of the RPM@LPS/PVA10-c-PP mesh was evaluated through in vivo experiments.					
36736174	5	7	theme	lyophilized	622:632	arg1	PVA10-c-PP					634:643	the lyophilized PVA10-c-PP	618:643	the lyophilized PVA10-c-PP	618:643	Subsequently, the lyophilized PVA10-c-PP was immersed in rapamycin (RPM)-loaded liposome solution until swelling equilibrated to obtain the anti-adhesion mesh RPM@LPS/PVA10-c-PP.					
36736174	1	8	theme	hernia	170:175	arg1	repair					177:182	hernia repair	170:182	hernia repair	170:182	Polypropylene (PP) mesh has been widely used in hernia repair as prosthesis material owing to its excellent balanced biocompatibility and mechanical properties.					
36736174	7	9	theme	excellent	976:984	arg1	cytocompatibility					986:1002	the excellent cytocompatibility	972:1002	the excellent cytocompatibility	972:1002	In vitro cell tests revealed the excellent cytocompatibility and the potential to inhibit cell adhesion of the modified PP mesh.					
36736174	2	10	theme	abdominal	292:300	arg1	adhesion					302:309	abdominal adhesion	292:309	abdominal adhesion between the visceral and PP mesh	292:342	However, abdominal adhesion between the visceral and PP mesh is still a major problem.					
36736174	2	10	theme	abdominal	292:300	arg1	problem					361:367	a major problem	353:367	a major problem	353:367	However, abdominal adhesion between the visceral and PP mesh is still a major problem.					
36736174	8	11	theme	RPM	1115:1117	arg1	mesh					1134:1137	the RPM@LPS/PVA10-c-PP mesh	1111:1137	the RPM@LPS/PVA10-c-PP mesh	1111:1137	Moreover, the anti-adhesive effects of the RPM@LPS/PVA10-c-PP mesh was evaluated through in vivo experiments.					
36736174	7	12	theme	mesh	1066:1069	arg1	cytocompatibility					986:1002	the excellent cytocompatibility	972:1002	the excellent cytocompatibility	972:1002	In vitro cell tests revealed the excellent cytocompatibility and the potential to inhibit cell adhesion of the modified PP mesh.					
36736174	7	12	theme	mesh	1066:1069	arg1	potential					1012:1020	the potential to inhibit cell adhesion	1008:1045	the potential to inhibit cell adhesion	1008:1045	In vitro cell tests revealed the excellent cytocompatibility and the potential to inhibit cell adhesion of the modified PP mesh.					
36736174	8	13	theme	LPS/PVA10-c-PP	1119:1132	arg1	mesh					1134:1137	the RPM@LPS/PVA10-c-PP mesh	1111:1137	the RPM@LPS/PVA10-c-PP mesh	1111:1137	Moreover, the anti-adhesive effects of the RPM@LPS/PVA10-c-PP mesh was evaluated through in vivo experiments.					
36736174	11	14	dep	showed	1512:1517	arg1	surrounded					1588:1597	surrounded	1588:1597	surrounded the PP filaments as compared to the native PP	1588:1643	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	7	15	theme	cell	952:955	arg1	tests					957:961	In vitro cell tests	943:961	In vitro cell tests	943:961	In vitro cell tests revealed the excellent cytocompatibility and the potential to inhibit cell adhesion of the modified PP mesh.					
36736174	1	16	theme	Polypropylene	122:134	arg1	mesh					141:144	Polypropylene (PP) mesh	122:144	Polypropylene (PP) mesh	122:144	Polypropylene (PP) mesh has been widely used in hernia repair as prosthesis material owing to its excellent balanced biocompatibility and mechanical properties.					
36736174	1	16	theme	Polypropylene	122:134	arg1	material					198:205	prosthesis material	187:205	prosthesis material owing to its excellent balanced biocompatibility and mechanical properties	187:280	Polypropylene (PP) mesh has been widely used in hernia repair as prosthesis material owing to its excellent balanced biocompatibility and mechanical properties.					
36736174	6	17	theme	mesh	865:868	arg1	surface					851:857	the surface	847:857	the surface of PP mesh	847:868	It was demonstrated that the hydrogel coating can stably fix on the surface of PP mesh even after immersed in PBS solution at 37 °C or 40 °C for up to 30 days.					
36736174	11	18	theme	native	1635:1640	arg1	PP					1642:1643	the native PP	1631:1643	the native PP	1631:1643	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	7	19	theme	In	943:944	arg1	tests					957:961	In vitro cell tests	943:961	In vitro cell tests	943:961	In vitro cell tests revealed the excellent cytocompatibility and the potential to inhibit cell adhesion of the modified PP mesh.					
36736174	1	20	used	used	162:165	arg2	material					198:205	prosthesis material	187:205	prosthesis material owing to its excellent balanced biocompatibility and mechanical properties	187:280	Polypropylene (PP) mesh has been widely used in hernia repair as prosthesis material owing to its excellent balanced biocompatibility and mechanical properties.					
36736174	1	20	used	used	162:165	arg2	mesh					141:144	Polypropylene (PP) mesh	122:144	Polypropylene (PP) mesh	122:144	Polypropylene (PP) mesh has been widely used in hernia repair as prosthesis material owing to its excellent balanced biocompatibility and mechanical properties.					
36736174	10	21	theme	@	1334:1334	arg1	mesh					1350:1353	RPM@LPS/PVA10-c-PP mesh	1331:1353	RPM@LPS/PVA10-c-PP mesh	1331:1353	At 30 days, the adhesion score of RPM@LPS/PVA10-c-PP mesh was 1.37 ± 0.75, however the original PP was 3 ± 0.71.					
36736174	13	22	theme	DATA	1775:1778	arg1	AVAILABILITY					1780:1791	DATA AVAILABILITY	1775:1791	DATA AVAILABILITY	1775:1791	DATA AVAILABILITY: Data will be made available on request.					
36736174	9	23	theme	@	1189:1189	arg1	mesh					1205:1208	The RPM@LPS/PVA10-c-PP mesh	1182:1208	The RPM@LPS/PVA10-c-PP mesh	1182:1208	The RPM@LPS/PVA10-c-PP mesh exhibited less adhesion than original PP mesh throughout the duration of implantation.					
36736174	3	24	theme	poly	421:424	arg1	hydrogel					447:454	poly(vinyl alcohol) (PVA) hydrogel	421:454	poly(vinyl alcohol) (PVA) hydrogel	421:454	Therefore, anti-adhesive PP mesh was designed with poly(vinyl alcohol) (PVA) hydrogel and liposomes drug delivery system.					
36736174	0	25	theme	delivery	105:112	arg1	system					114:119	drug delivery system	100:119	drug delivery system	100:119	Polypropylene composite hernia mesh with anti-adhesion layer composed of PVA hydrogel and liposomes drug delivery system.					
36736174	0	26	theme	anti-adhesion	41:53	arg1	layer					55:59	anti-adhesion layer	41:59	anti-adhesion layer composed of PVA hydrogel and liposomes drug delivery system	41:119	Polypropylene composite hernia mesh with anti-adhesion layer composed of PVA hydrogel and liposomes drug delivery system.					
36736174	8	27	dep	in	1161:1162	arg1	vivo					1164:1167	vivo	1164:1167	vivo	1164:1167	Moreover, the anti-adhesive effects of the RPM@LPS/PVA10-c-PP mesh was evaluated through in vivo experiments.					
36736174	6	28	dep	30	934:935	arg1	to					931:932	to	931:932	to	931:932	It was demonstrated that the hydrogel coating can stably fix on the surface of PP mesh even after immersed in PBS solution at 37 °C or 40 °C for up to 30 days.					
36736174	10	29	theme	mesh	1350:1353	arg1	score					1322:1326	the adhesion score	1309:1326	the adhesion score of RPM@LPS/PVA10-c-PP mesh	1309:1353	At 30 days, the adhesion score of RPM@LPS/PVA10-c-PP mesh was 1.37 ± 0.75, however the original PP was 3 ± 0.71.					
36736174	10	29	theme	mesh	1350:1353	arg1	1.37 ± 0.75					1359:1369	1.37 ± 0.75	1359:1369	1.37 ± 0.75	1359:1369	At 30 days, the adhesion score of RPM@LPS/PVA10-c-PP mesh was 1.37 ± 0.75, however the original PP was 3 ± 0.71.					
36736174	5	30	theme	RPM	763:765	arg1	LPS/PVA10-c-PP					767:780	the anti-adhesion mesh RPM@LPS/PVA10-c-PP	740:780	the anti-adhesion mesh RPM@LPS/PVA10-c-PP	740:780	Subsequently, the lyophilized PVA10-c-PP was immersed in rapamycin (RPM)-loaded liposome solution until swelling equilibrated to obtain the anti-adhesion mesh RPM@LPS/PVA10-c-PP.					
36736174	0	31	dep	hydrogel	77:84	arg1	system					114:119	drug delivery system	100:119	drug delivery system	100:119	Polypropylene composite hernia mesh with anti-adhesion layer composed of PVA hydrogel and liposomes drug delivery system.					
36736174	4	32	theme	hydrogel	503:510	arg1	coating					512:518	PVA hydrogel coating	499:518	PVA hydrogel coating	499:518	First, PVA hydrogel coating was formed on the surface of PP mesh with freezing-thawing processing cycles (FTP).					
36736174	10	33	theme	original	1384:1391	arg1	PP					1393:1394	the original PP	1380:1394	the original PP	1380:1394	At 30 days, the adhesion score of RPM@LPS/PVA10-c-PP mesh was 1.37 ± 0.75, however the original PP was 3 ± 0.71.					
36736174	0	34	theme	hernia	24:29	arg1	mesh					31:34	hernia mesh	24:34	hernia mesh	24:34	Polypropylene composite hernia mesh with anti-adhesion layer composed of PVA hydrogel and liposomes drug delivery system.					
36736174	9	35	theme	PP	1248:1249	arg1	mesh					1251:1254	original PP mesh	1239:1254	original PP mesh	1239:1254	The RPM@LPS/PVA10-c-PP mesh exhibited less adhesion than original PP mesh throughout the duration of implantation.					
36736174	3	36	theme	vinyl	426:430	arg1	poly					421:424	poly	421:424	poly(vinyl alcohol) (PVA) hydrogel	421:454	Therefore, anti-adhesive PP mesh was designed with poly(vinyl alcohol) (PVA) hydrogel and liposomes drug delivery system.					
36736174	3	36	theme	vinyl	426:430	arg1	alcohol					432:438	vinyl alcohol	426:438	vinyl alcohol	426:438	Therefore, anti-adhesive PP mesh was designed with poly(vinyl alcohol) (PVA) hydrogel and liposomes drug delivery system.					
36736174	7	37	theme	PP	1063:1064	arg1	mesh					1066:1069	the modified PP mesh	1050:1069	the modified PP mesh	1050:1069	In vitro cell tests revealed the excellent cytocompatibility and the potential to inhibit cell adhesion of the modified PP mesh.					
36736174	12	38	theme	anti-adhesion	1750:1762	arg1	treatment					1764:1772	anti-adhesion treatment	1750:1772	anti-adhesion treatment	1750:1772	The current findings manifested that this type of RPM@LPS/PVA10-c-PP might be a potential candidate for anti-adhesion treatment.					
36736174	12	39	theme	RPM	1696:1698	arg1	LPS/PVA10-c-PP					1700:1713	RPM@LPS/PVA10-c-PP	1696:1713	RPM@LPS/PVA10-c-PP	1696:1713	The current findings manifested that this type of RPM@LPS/PVA10-c-PP might be a potential candidate for anti-adhesion treatment.					
36736174	3	40	theme	anti-adhesive	381:393	arg1	mesh					398:401	anti-adhesive PP mesh	381:401	anti-adhesive PP mesh	381:401	Therefore, anti-adhesive PP mesh was designed with poly(vinyl alcohol) (PVA) hydrogel and liposomes drug delivery system.					
36736174	5	41	theme	anti-adhesion	744:756	arg1	LPS/PVA10-c-PP					767:780	the anti-adhesion mesh RPM@LPS/PVA10-c-PP	740:780	the anti-adhesion mesh RPM@LPS/PVA10-c-PP	740:780	Subsequently, the lyophilized PVA10-c-PP was immersed in rapamycin (RPM)-loaded liposome solution until swelling equilibrated to obtain the anti-adhesion mesh RPM@LPS/PVA10-c-PP.					
36736174	12	42	theme	LPS/PVA10-c-PP	1700:1713	arg1	type					1688:1691	this type	1683:1691	this type of RPM@LPS/PVA10-c-PP	1683:1713	The current findings manifested that this type of RPM@LPS/PVA10-c-PP might be a potential candidate for anti-adhesion treatment.					
36736174	12	42	theme	LPS/PVA10-c-PP	1700:1713	arg1	candidate					1736:1744	a potential candidate	1724:1744	a potential candidate for anti-adhesion treatment	1724:1772	The current findings manifested that this type of RPM@LPS/PVA10-c-PP might be a potential candidate for anti-adhesion treatment.					
36736174	11	43	theme	staining	1463:1470	arg1	results					1427:1433	the results	1423:1433	the results of H&E and Masson trichrome staining	1423:1470	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	9	44	theme	implantation	1283:1294	arg1	duration					1271:1278	the duration	1267:1278	the duration of implantation	1267:1294	The RPM@LPS/PVA10-c-PP mesh exhibited less adhesion than original PP mesh throughout the duration of implantation.					
36736174	4	45	theme	PP	549:550	arg1	mesh					552:555	PP mesh	549:555	PP mesh with freezing-thawing processing cycles (FTP)	549:601	First, PVA hydrogel coating was formed on the surface of PP mesh with freezing-thawing processing cycles (FTP).					
36736174	11	46	theme	LPS/PVA10-c-PP	1492:1505	arg1	mesh					1507:1510	the RPM@LPS/PVA10-c-PP mesh	1484:1510	the RPM@LPS/PVA10-c-PP mesh	1484:1510	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	2	47	theme	major	355:359	arg1	adhesion					302:309	abdominal adhesion	292:309	abdominal adhesion between the visceral and PP mesh	292:342	However, abdominal adhesion between the visceral and PP mesh is still a major problem.					
36736174	2	47	theme	major	355:359	arg1	problem					361:367	a major problem	353:367	a major problem	353:367	However, abdominal adhesion between the visceral and PP mesh is still a major problem.					
36736174	6	48	theme	PP	862:863	arg1	mesh					865:868	PP mesh	862:868	PP mesh	862:868	It was demonstrated that the hydrogel coating can stably fix on the surface of PP mesh even after immersed in PBS solution at 37 °C or 40 °C for up to 30 days.					
36736174	10	49	from	days	1303:1306	arg1	score					1322:1326	the adhesion score	1309:1326	the adhesion score of RPM@LPS/PVA10-c-PP mesh	1309:1353	At 30 days, the adhesion score of RPM@LPS/PVA10-c-PP mesh was 1.37 ± 0.75, however the original PP was 3 ± 0.71.					
36736174	10	49	from	days	1303:1306	arg1	1.37 ± 0.75					1359:1369	1.37 ± 0.75	1359:1369	1.37 ± 0.75	1359:1369	At 30 days, the adhesion score of RPM@LPS/PVA10-c-PP mesh was 1.37 ± 0.75, however the original PP was 3 ± 0.71.					
36736174	11	50	theme	inflammation	1528:1539	arg1	response					1541:1548	slighter inflammation response	1519:1548	slighter inflammation response	1519:1548	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	7	51	theme	cell	1033:1036	arg1	adhesion					1038:1045	cell adhesion	1033:1045	cell adhesion	1033:1045	In vitro cell tests revealed the excellent cytocompatibility and the potential to inhibit cell adhesion of the modified PP mesh.					
36736174	11	52	theme	RPM	1488:1490	arg1	mesh					1507:1510	the RPM@LPS/PVA10-c-PP mesh	1484:1510	the RPM@LPS/PVA10-c-PP mesh	1484:1510	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	1	53	theme	balanced	230:237	arg1	biocompatibility					239:254	its excellent balanced biocompatibility	216:254	its excellent balanced biocompatibility	216:254	Polypropylene (PP) mesh has been widely used in hernia repair as prosthesis material owing to its excellent balanced biocompatibility and mechanical properties.					
36736174	11	54	theme	looser	1566:1571	arg1	tissue					1581:1586	significant looser fibrous tissue	1554:1586	significant looser fibrous tissue	1554:1586	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	8	55	theme	mesh	1134:1137	arg1	effects					1100:1106	the anti-adhesive effects	1082:1106	the anti-adhesive effects of the RPM@LPS/PVA10-c-PP mesh	1082:1137	Moreover, the anti-adhesive effects of the RPM@LPS/PVA10-c-PP mesh was evaluated through in vivo experiments.					
36736174	11	56	theme	Masson	1446:1451	arg1	staining					1463:1470	H&E and Masson trichrome staining	1438:1470	staining	1463:1470	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	11	57	theme	H&E	1438:1440	arg1	staining					1463:1470	H&E and Masson trichrome staining	1438:1470	staining	1463:1470	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	6	58	theme	PBS	893:895	arg1	solution					897:904	PBS solution	893:904	PBS solution	893:904	It was demonstrated that the hydrogel coating can stably fix on the surface of PP mesh even after immersed in PBS solution at 37 °C or 40 °C for up to 30 days.					
36736174	11	59	theme	trichrome	1453:1461	arg1	staining					1463:1470	H&E and Masson trichrome staining	1438:1470	staining	1463:1470	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	4	60	theme	processing	579:588	arg1	FTP					598:600	FTP	598:600	FTP	598:600	First, PVA hydrogel coating was formed on the surface of PP mesh with freezing-thawing processing cycles (FTP).					
36736174	4	60	theme	processing	579:588	arg1	cycles					590:595	freezing-thawing processing cycles	562:595	freezing-thawing processing cycles (FTP)	562:601	First, PVA hydrogel coating was formed on the surface of PP mesh with freezing-thawing processing cycles (FTP).					
36736174	1	61	theme	prosthesis	187:196	arg1	mesh					141:144	Polypropylene (PP) mesh	122:144	Polypropylene (PP) mesh	122:144	Polypropylene (PP) mesh has been widely used in hernia repair as prosthesis material owing to its excellent balanced biocompatibility and mechanical properties.					
36736174	1	61	theme	prosthesis	187:196	arg1	material					198:205	prosthesis material	187:205	prosthesis material owing to its excellent balanced biocompatibility and mechanical properties	187:280	Polypropylene (PP) mesh has been widely used in hernia repair as prosthesis material owing to its excellent balanced biocompatibility and mechanical properties.					
36736174	4	62	with	mesh	552:555	arg1	FTP					598:600	FTP	598:600	FTP	598:600	First, PVA hydrogel coating was formed on the surface of PP mesh with freezing-thawing processing cycles (FTP).					
36736174	4	62	with	mesh	552:555	arg1	cycles					590:595	freezing-thawing processing cycles	562:595	freezing-thawing processing cycles (FTP)	562:601	First, PVA hydrogel coating was formed on the surface of PP mesh with freezing-thawing processing cycles (FTP).					
36736174	8	63	theme	@	1118:1118	arg1	mesh					1134:1137	the RPM@LPS/PVA10-c-PP mesh	1111:1137	the RPM@LPS/PVA10-c-PP mesh	1111:1137	Moreover, the anti-adhesive effects of the RPM@LPS/PVA10-c-PP mesh was evaluated through in vivo experiments.					
36736174	0	64	theme	PVA	73:75	arg1	hydrogel					77:84	PVA hydrogel	73:84	PVA hydrogel	73:84	Polypropylene composite hernia mesh with anti-adhesion layer composed of PVA hydrogel and liposomes drug delivery system.					
36736174	9	65	theme	RPM	1186:1188	arg1	mesh					1205:1208	The RPM@LPS/PVA10-c-PP mesh	1182:1208	The RPM@LPS/PVA10-c-PP mesh	1182:1208	The RPM@LPS/PVA10-c-PP mesh exhibited less adhesion than original PP mesh throughout the duration of implantation.					
36736174	10	66	theme	RPM	1331:1333	arg1	mesh					1350:1353	RPM@LPS/PVA10-c-PP mesh	1331:1353	RPM@LPS/PVA10-c-PP mesh	1331:1353	At 30 days, the adhesion score of RPM@LPS/PVA10-c-PP mesh was 1.37 ± 0.75, however the original PP was 3 ± 0.71.					
36736174	0	67	theme	drug	100:103	arg1	system					114:119	drug delivery system	100:119	drug delivery system	100:119	Polypropylene composite hernia mesh with anti-adhesion layer composed of PVA hydrogel and liposomes drug delivery system.					
36736174	9	68	theme	LPS/PVA10-c-PP	1190:1203	arg1	mesh					1205:1208	The RPM@LPS/PVA10-c-PP mesh	1182:1208	The RPM@LPS/PVA10-c-PP mesh	1182:1208	The RPM@LPS/PVA10-c-PP mesh exhibited less adhesion than original PP mesh throughout the duration of implantation.					
36736174	5	69	theme	-loaded	676:682	arg1	solution					693:700	rapamycin (RPM)-loaded liposome solution	661:700	rapamycin (RPM)-loaded liposome solution	661:700	Subsequently, the lyophilized PVA10-c-PP was immersed in rapamycin (RPM)-loaded liposome solution until swelling equilibrated to obtain the anti-adhesion mesh RPM@LPS/PVA10-c-PP.					
36736174	8	70	theme	in	1161:1162	arg1	experiments					1169:1179	in vivo experiments	1161:1179	in vivo experiments	1161:1179	Moreover, the anti-adhesive effects of the RPM@LPS/PVA10-c-PP mesh was evaluated through in vivo experiments.					
36736174	3	71	dep	hydrogel	447:454	arg1	system					484:489	drug delivery system	470:489	drug delivery system	470:489	Therefore, anti-adhesive PP mesh was designed with poly(vinyl alcohol) (PVA) hydrogel and liposomes drug delivery system.					
36736174	5	72	theme	mesh	758:761	arg1	LPS/PVA10-c-PP					767:780	the anti-adhesion mesh RPM@LPS/PVA10-c-PP	740:780	the anti-adhesion mesh RPM@LPS/PVA10-c-PP	740:780	Subsequently, the lyophilized PVA10-c-PP was immersed in rapamycin (RPM)-loaded liposome solution until swelling equilibrated to obtain the anti-adhesion mesh RPM@LPS/PVA10-c-PP.					
36736174	10	73	theme	LPS/PVA10-c-PP	1335:1348	arg1	mesh					1350:1353	RPM@LPS/PVA10-c-PP mesh	1331:1353	RPM@LPS/PVA10-c-PP mesh	1331:1353	At 30 days, the adhesion score of RPM@LPS/PVA10-c-PP mesh was 1.37 ± 0.75, however the original PP was 3 ± 0.71.					
36736174	5	74	theme	@	766:766	arg1	LPS/PVA10-c-PP					767:780	the anti-adhesion mesh RPM@LPS/PVA10-c-PP	740:780	the anti-adhesion mesh RPM@LPS/PVA10-c-PP	740:780	Subsequently, the lyophilized PVA10-c-PP was immersed in rapamycin (RPM)-loaded liposome solution until swelling equilibrated to obtain the anti-adhesion mesh RPM@LPS/PVA10-c-PP.					
36736174	9	75	theme	original	1239:1246	arg1	mesh					1251:1254	original PP mesh	1239:1254	original PP mesh	1239:1254	The RPM@LPS/PVA10-c-PP mesh exhibited less adhesion than original PP mesh throughout the duration of implantation.					
36736174	3	76	theme	delivery	475:482	arg1	system					484:489	drug delivery system	470:489	drug delivery system	470:489	Therefore, anti-adhesive PP mesh was designed with poly(vinyl alcohol) (PVA) hydrogel and liposomes drug delivery system.					
36736174	7	77	theme	modified	1054:1061	arg1	mesh					1066:1069	the modified PP mesh	1050:1069	the modified PP mesh	1050:1069	In vitro cell tests revealed the excellent cytocompatibility and the potential to inhibit cell adhesion of the modified PP mesh.					
36736174	6	78	theme	hydrogel	812:819	arg1	coating					821:827	the hydrogel coating	808:827	the hydrogel coating	808:827	It was demonstrated that the hydrogel coating can stably fix on the surface of PP mesh even after immersed in PBS solution at 37 °C or 40 °C for up to 30 days.					
36736174	11	79	theme	fibrous	1573:1579	arg1	tissue					1581:1586	significant looser fibrous tissue	1554:1586	significant looser fibrous tissue	1554:1586	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	5	80	theme	liposome	684:691	arg1	solution					693:700	rapamycin (RPM)-loaded liposome solution	661:700	rapamycin (RPM)-loaded liposome solution	661:700	Subsequently, the lyophilized PVA10-c-PP was immersed in rapamycin (RPM)-loaded liposome solution until swelling equilibrated to obtain the anti-adhesion mesh RPM@LPS/PVA10-c-PP.					
36736174	3	81	theme	PP	395:396	arg1	mesh					398:401	anti-adhesive PP mesh	381:401	anti-adhesive PP mesh	381:401	Therefore, anti-adhesive PP mesh was designed with poly(vinyl alcohol) (PVA) hydrogel and liposomes drug delivery system.					
36736174	4	82	theme	PVA	499:501	arg1	coating					512:518	PVA hydrogel coating	499:518	PVA hydrogel coating	499:518	First, PVA hydrogel coating was formed on the surface of PP mesh with freezing-thawing processing cycles (FTP).					
36736174	0	83	with	composite	14:22	arg1	layer					55:59	anti-adhesion layer	41:59	anti-adhesion layer composed of PVA hydrogel and liposomes drug delivery system	41:119	Polypropylene composite hernia mesh with anti-adhesion layer composed of PVA hydrogel and liposomes drug delivery system.					
36736174	2	84	theme	PP	336:337	arg1	mesh					339:342	the visceral and PP mesh	319:342	mesh	339:342	However, abdominal adhesion between the visceral and PP mesh is still a major problem.					
36736174	12	85	theme	potential	1726:1734	arg1	type					1688:1691	this type	1683:1691	this type of RPM@LPS/PVA10-c-PP	1683:1713	The current findings manifested that this type of RPM@LPS/PVA10-c-PP might be a potential candidate for anti-adhesion treatment.					
36736174	12	85	theme	potential	1726:1734	arg1	candidate					1736:1744	a potential candidate	1724:1744	a potential candidate for anti-adhesion treatment	1724:1772	The current findings manifested that this type of RPM@LPS/PVA10-c-PP might be a potential candidate for anti-adhesion treatment.					
36736174	12	86	theme	@	1699:1699	arg1	LPS/PVA10-c-PP					1700:1713	RPM@LPS/PVA10-c-PP	1696:1713	RPM@LPS/PVA10-c-PP	1696:1713	The current findings manifested that this type of RPM@LPS/PVA10-c-PP might be a potential candidate for anti-adhesion treatment.					
36736174	4	87	theme	mesh	552:555	arg1	surface					538:544	the surface	534:544	the surface of PP mesh with freezing-thawing processing cycles (FTP)	534:601	First, PVA hydrogel coating was formed on the surface of PP mesh with freezing-thawing processing cycles (FTP).					
36736174	11	88	theme	PP	1603:1604	arg1	filaments					1606:1614	the PP filaments	1599:1614	the PP filaments	1599:1614	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	11	89	theme	@	1491:1491	arg1	mesh					1507:1510	the RPM@LPS/PVA10-c-PP mesh	1484:1510	the RPM@LPS/PVA10-c-PP mesh	1484:1510	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	2	90	theme	visceral	323:330	arg1	mesh					339:342	the visceral and PP mesh	319:342	mesh	339:342	However, abdominal adhesion between the visceral and PP mesh is still a major problem.					
36736174	4	91	theme	freezing-thawing	562:577	arg1	FTP					598:600	FTP	598:600	FTP	598:600	First, PVA hydrogel coating was formed on the surface of PP mesh with freezing-thawing processing cycles (FTP).					
36736174	4	91	theme	freezing-thawing	562:577	arg1	cycles					590:595	freezing-thawing processing cycles	562:595	freezing-thawing processing cycles (FTP)	562:601	First, PVA hydrogel coating was formed on the surface of PP mesh with freezing-thawing processing cycles (FTP).					
36736174	11	92	theme	slighter	1519:1526	arg1	response					1541:1548	slighter inflammation response	1519:1548	slighter inflammation response	1519:1548	Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA10-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP.					
36736174	1	93	theme	excellent	220:228	arg1	biocompatibility					239:254	its excellent balanced biocompatibility	216:254	its excellent balanced biocompatibility	216:254	Polypropylene (PP) mesh has been widely used in hernia repair as prosthesis material owing to its excellent balanced biocompatibility and mechanical properties.					
35775198	9	0	from	protein	2336:2342	arg1	scaffolds					2366:2374	the functionalized scaffolds	2347:2374	the functionalized scaffolds	2347:2374	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	10	1	dep	microstructures	2719:2733	arg1	the					2715:2717	the	2715:2717	the	2715:2717	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	6	2	theme	better	1515:1520	arg1	kinetics					1529:1536	better enzyme kinetics	1515:1536	better enzyme kinetics	1515:1536	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	5	3	theme	surface	1308:1314	arg1	energy					1316:1321	surface energy	1308:1321	surface energy (γAB) from 6.35 to 12.54 mN m-1	1308:1353	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	5	3	theme	surface	1308:1314	arg1	γAB					1324:1326	γAB	1324:1326	γAB	1324:1326	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	4	4	theme	surface	1128:1134	arg1	calculations					1143:1154	surface energy calculations	1128:1154	surface energy calculations following the Van Oss-Chaudhury-Good method	1128:1198	Surface chemical compositional analysis using XPS and ATR FT-IR confirms the incorporation of aldehyde functionality after functionalization, which is corroborated by surface energy calculations following the Van Oss-Chaudhury-Good method.					
35775198	1	5	contain	having	197:202	arg1	Bioscaffolds					184:195	Bioscaffolds	184:195	Bioscaffolds having electrically conducting polymers (CPs)	184:241	Bioscaffolds having electrically conducting polymers (CPs) have become increasingly relevant in tissue engineering (TE) because of their ability to regulate conductivity and promote biological function.					
35775198	1	5	contain	having	197:202	arg2	CPs					238:240	CPs	238:240	CPs	238:240	Bioscaffolds having electrically conducting polymers (CPs) have become increasingly relevant in tissue engineering (TE) because of their ability to regulate conductivity and promote biological function.					
35775198	1	5	contain	having	197:202	arg2	polymers					228:235	electrically conducting polymers	204:235	electrically conducting polymers (CPs)	204:241	Bioscaffolds having electrically conducting polymers (CPs) have become increasingly relevant in tissue engineering (TE) because of their ability to regulate conductivity and promote biological function.					
35775198	5	6	from	hydrophilicity	1258:1271	arg1	terms					1276:1280	terms	1276:1280	terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1	1276:1353	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	3	7	from	dispersion	687:696	arg1	matrix					704:709	Ch matrix	701:709	Ch matrix	701:709	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	5	8	from	component	1295:1303	arg1	m-1					1351:1353	6.35 to 12.54 mN m-1	1334:1353	6.35 to 12.54 mN m-1	1334:1353	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	8	9	theme	significant	2121:2131	arg1	remodeling					2139:2148	significant actin remodeling	2121:2148	significant actin remodeling with neurite-like projections	2121:2178	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	1	10	theme	conducting	217:226	arg1	polymers					228:235	electrically conducting polymers	204:235	electrically conducting polymers (CPs)	204:241	Bioscaffolds having electrically conducting polymers (CPs) have become increasingly relevant in tissue engineering (TE) because of their ability to regulate conductivity and promote biological function.					
35775198	1	10	theme	conducting	217:226	arg1	CPs					238:240	CPs	238:240	CPs	238:240	Bioscaffolds having electrically conducting polymers (CPs) have become increasingly relevant in tissue engineering (TE) because of their ability to regulate conductivity and promote biological function.					
35775198	7	11	theme	stem	1886:1889	arg1	ADMSCs					1898:1903	ADMSCs	1898:1903	ADMSCs	1898:1903	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	7	11	theme	stem	1886:1889	arg1	cells					1891:1895	primary adipose derived mesenchymal stem cells	1850:1895	primary adipose derived mesenchymal stem cells (ADMSCs)	1850:1904	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	3	12	theme	required	802:809	arg1	properties					827:836	required physicochemical properties	802:836	required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test	802:958	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	7	13	theme	Surface	1722:1728	arg1	functionalization					1730:1746	Surface functionalization	1722:1746	Surface functionalization	1722:1746	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	4	14	theme	chemical	969:976	arg1	analysis					992:999	Surface chemical compositional analysis	961:999	Surface chemical compositional analysis using XPS and ATR FT-IR	961:1023	Surface chemical compositional analysis using XPS and ATR FT-IR confirms the incorporation of aldehyde functionality after functionalization, which is corroborated by surface energy calculations following the Van Oss-Chaudhury-Good method.					
35775198	5	15	dep	12.54	1342:1346	arg1	to					1339:1340	to	1339:1340	to	1339:1340	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	4	16	theme	Oss-Chaudhury-Good	1174:1191	arg1	method					1193:1198	the Van Oss-Chaudhury-Good method	1166:1198	the Van Oss-Chaudhury-Good method	1166:1198	Surface chemical compositional analysis using XPS and ATR FT-IR confirms the incorporation of aldehyde functionality after functionalization, which is corroborated by surface energy calculations following the Van Oss-Chaudhury-Good method.					
35775198	9	17	theme	neuron	2306:2311	arg1	protein					2336:2342	early neuron specific β III tubulin protein	2300:2342	early neuron specific β III tubulin protein on the functionalized scaffolds	2300:2374	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	0	18	theme	Stem	152:155	arg1	Cells					157:161	Primary Adipose Derived Mesenchymal Stem Cells	116:161	Primary Adipose Derived Mesenchymal Stem Cells	116:161	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	4	19	theme	compositional	978:990	arg1	analysis					992:999	Surface chemical compositional analysis	961:999	Surface chemical compositional analysis using XPS and ATR FT-IR	961:1023	Surface chemical compositional analysis using XPS and ATR FT-IR confirms the incorporation of aldehyde functionality after functionalization, which is corroborated by surface energy calculations following the Van Oss-Chaudhury-Good method.					
35775198	6	20	theme	constants	1568:1576	arg1	reduction					1545:1553	a reduction	1543:1553	a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM	1543:1604	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	9	21	theme	β	2322:2322	arg1	protein					2336:2342	early neuron specific β III tubulin protein	2300:2342	early neuron specific β III tubulin protein on the functionalized scaffolds	2300:2374	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	8	22	theme	fibroblast	2046:2055	arg1	bFGF					2072:2075	bFGF	2072:2075	bFGF	2072:2075	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	8	22	theme	fibroblast	2046:2055	arg1	factor					2064:2069	each basic fibroblast growth factor	2035:2069	each basic fibroblast growth factor (bFGF)	2035:2076	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	6	23	from	%	1509:1509	arg1	activity					1487:1494	enzyme activity	1480:1494	enzyme activity from 67 to 94%	1480:1509	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	6	23	from	%	1509:1509	arg1	kinetics					1529:1536	better enzyme kinetics	1515:1536	better enzyme kinetics	1515:1536	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	6	23	from	%	1509:1509	arg1	improvement					1465:1475	improvement	1465:1475	improvement in enzyme activity from 67 to 94%	1465:1509	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	2	24	theme	regeneration	621:632	arg1	applications					634:645	potential neural regeneration applications	604:645	potential neural regeneration applications	604:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	10	25	theme	microchanneled	2662:2675	arg1	structures					2693:2702	microchanneled or conduit-like structures	2662:2702	microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves	2662:2780	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	10	26	theme	neural	2593:2598	arg1	regeneration					2600:2611	electrically stimulated neural regeneration	2569:2611	electrically stimulated neural regeneration	2569:2611	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	9	27	theme	tubulin	2328:2334	arg1	protein					2336:2342	early neuron specific β III tubulin protein	2300:2342	early neuron specific β III tubulin protein on the functionalized scaffolds	2300:2374	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	8	28	theme	factor	2064:2069	arg1	ng/mL					2026:2030	10 ng/mL	2023:2030	10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF)	2023:2110	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	6	29	theme	protein-biomaterial	1628:1646	arg1	interactions					1648:1659	favorable protein-biomaterial interactions	1618:1659	favorable protein-biomaterial interactions	1618:1659	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	3	30	theme	mechanical	899:908	arg1	test					919:922	mechanical strength test	899:922	mechanical strength test	899:922	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	3	31	dep	in	929:930	arg1	vitro					932:936	vitro	932:936	vitro	932:936	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	2	32	theme	potential	604:612	arg1	applications					634:645	potential neural regeneration applications	604:645	potential neural regeneration applications	604:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	1	33	from	relevant	268:275	arg1	TE					300:301	TE	300:301	TE	300:301	Bioscaffolds having electrically conducting polymers (CPs) have become increasingly relevant in tissue engineering (TE) because of their ability to regulate conductivity and promote biological function.					
35775198	1	33	from	relevant	268:275	arg1	engineering					287:297	tissue engineering	280:297	tissue engineering (TE)	280:302	Bioscaffolds having electrically conducting polymers (CPs) have become increasingly relevant in tissue engineering (TE) because of their ability to regulate conductivity and promote biological function.					
35775198	8	34	theme	growth	2092:2097	arg1	EGF					2107:2109	EGF	2107:2109	EGF	2107:2109	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	8	34	theme	growth	2092:2097	arg1	factor					2099:2104	epidermal growth factor	2082:2104	epidermal growth factor (EGF)	2082:2110	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	6	35	dep	13.81	1597:1601	arg1	to					1594:1595	to	1594:1595	to	1594:1595	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	9	36	theme	functionalized	2351:2364	arg1	scaffolds					2366:2374	the functionalized scaffolds	2347:2374	the functionalized scaffolds	2347:2374	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	7	37	theme	primary	1850:1856	arg1	ADMSCs					1898:1903	ADMSCs	1898:1903	ADMSCs	1898:1903	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	7	37	theme	primary	1850:1856	arg1	cells					1891:1895	primary adipose derived mesenchymal stem cells	1850:1895	primary adipose derived mesenchymal stem cells (ADMSCs)	1850:1904	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	2	38	theme	nanocomposites	497:510	arg1	scaffold					512:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold	430:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	430:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	10	39	theme	conduit-like	2680:2691	arg1	structures					2693:2702	microchanneled or conduit-like structures	2662:2702	microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves	2662:2780	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	3	40	theme	biological	763:772	arg1	functions					774:782	desirable biological functions	753:782	desirable biological functions	753:782	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	6	41	theme	Ch	1439:1440	arg1	scaffolds					1442:1450	Ch scaffolds	1439:1450	Ch scaffolds	1439:1450	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	6	42	from	improvement	1465:1475	arg1	activity					1487:1494	enzyme activity	1480:1494	enzyme activity from 67 to 94%	1480:1509	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	6	42	from	improvement	1465:1475	arg1	%					1509:1509	67 to 94%	1501:1509	67 to 94%	1501:1509	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	8	43	theme	differentiating	1990:2004	arg1	media					2006:2010	differentiating media	1990:2010	differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF)	1990:2110	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	2	44	theme	surface	544:550	arg1	approach					570:577	a simple one-step surface functionalization approach	526:577	a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	526:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	0	45	theme	Urease	167:172	arg1	Activity					174:181	Urease Activity	167:181	Urease Activity	167:181	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	3	46	theme	current-voltage	864:878	arg1	measurement					886:896	current-voltage (I-V) measurement	864:896	current-voltage (I-V) measurement	864:896	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	0	47	theme	Primary	116:122	arg1	Cells					157:161	Primary Adipose Derived Mesenchymal Stem Cells	116:161	Primary Adipose Derived Mesenchymal Stem Cells	116:161	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	3	48	dep	properties	827:836	arg1	evidenced					841:849	evidenced	841:849	evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test	841:958	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	6	49	dep	PNF	1435:1437	arg1	demonstrated					1452:1463	demonstrated	1452:1463	demonstrated	1452:1463	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	2	50	theme	simple	528:533	arg1	approach					570:577	a simple one-step surface functionalization approach	526:577	a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	526:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	9	51	theme	acidic	2402:2407	arg1	GFAP					2418:2421	GFAP	2418:2421	GFAP	2418:2421	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	9	51	theme	acidic	2402:2407	arg1	protein					2409:2415	glial fibrillary acidic protein	2385:2415	glial fibrillary acidic protein (GFAP) expression	2385:2433	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	0	52	theme	Derived	132:138	arg1	Cells					157:161	Primary Adipose Derived Mesenchymal Stem Cells	116:161	Primary Adipose Derived Mesenchymal Stem Cells	116:161	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	0	53	theme	Chitosan	46:53	arg1	Nanocomposite					55:67	Chitosan Nanocomposite	46:67	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.	0:182	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	8	54	theme	functionalized	2187:2200	arg1	scaffolds					2202:2210	the functionalized scaffolds	2183:2210	the functionalized scaffolds	2183:2210	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	8	55	theme	epidermal	2082:2090	arg1	EGF					2107:2109	EGF	2107:2109	EGF	2107:2109	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	8	55	theme	epidermal	2082:2090	arg1	factor					2099:2104	epidermal growth factor	2082:2104	epidermal growth factor (EGF)	2082:2110	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	10	56	theme	peripheral	2764:2773	arg1	nerves					2775:2780	peripheral nerves	2764:2780	peripheral nerves	2764:2780	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	0	57	theme	Surface	0:6	arg1	Nanofibers					35:44	Surface Functionalized Polyaniline Nanofibers	0:44	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.	0:182	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	6	58	dep	demonstrated	1452:1463	arg1	establishing					1665:1676	establishing	1665:1676	establishing them as biologically perceptible materials	1665:1719	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	6	58	dep	demonstrated	1452:1463	arg1	indicating					1607:1616	indicating	1607:1616	indicating favorable protein-biomaterial interactions	1607:1659	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	8	59	theme	architecture	1960:1971	arg1	assessment					1973:1982	Cytoskeletal architecture assessment	1947:1982	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF)	1947:2110	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	7	60	theme	improved	1757:1764	arg1	interactions					1783:1794	improved cell-biomaterial interactions	1757:1794	improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility	1757:1944	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	5	61	theme	surface	1381:1387	arg1	polarity					1389:1396	surface polarity	1381:1396	surface polarity from 13.61 to 22.54%	1381:1417	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	2	62	theme	polyaniline	443:453	arg1	scaffold					512:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold	430:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	430:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	0	63	theme	Polyaniline	23:33	arg1	Nanofibers					35:44	Surface Functionalized Polyaniline Nanofibers	0:44	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.	0:182	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	5	64	theme	polar	1289:1293	arg1	component					1295:1303	the polar component	1285:1303	the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1	1285:1353	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	10	65	theme	smart	2550:2554	arg1	scaffold					2556:2563	a smart scaffold	2548:2563	a smart scaffold for electrically stimulated neural regeneration	2548:2611	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	2	66	theme	Ch	493:494	arg1	scaffold					512:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold	430:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	430:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	8	67	theme	neurite-like	2155:2166	arg1	projections					2168:2178	neurite-like projections	2155:2178	neurite-like projections	2155:2178	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	5	68	theme	surface	1250:1256	arg1	hydrophilicity					1258:1271	surface hydrophilicity	1250:1271	surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1	1250:1353	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	5	69	dep	22.54	1412:1416	arg1	to					1409:1410	to	1409:1410	to	1409:1410	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	2	70	theme	chitosan	483:490	arg1	scaffold					512:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold	430:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	430:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	3	71	theme	%	680:680	arg1	concentration					725:737	an optimal concentration	714:737	an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test	714:958	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	3	71	theme	%	680:680	arg1	dispersion					687:696	4 wt % PNFs dispersion	675:696	4 wt % PNFs dispersion in Ch matrix	675:709	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	8	72	with	remodeling	2139:2148	arg1	projections					2168:2178	neurite-like projections	2155:2178	neurite-like projections	2155:2178	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	4	73	theme	ATR	1015:1017	arg1	FT-IR					1019:1023	ATR FT-IR	1015:1023	ATR FT-IR	1015:1023	Surface chemical compositional analysis using XPS and ATR FT-IR confirms the incorporation of aldehyde functionality after functionalization, which is corroborated by surface energy calculations following the Van Oss-Chaudhury-Good method.					
35775198	4	74	theme	aldehyde	1055:1062	arg1	functionality					1064:1076	aldehyde functionality	1055:1076	aldehyde functionality	1055:1076	Surface chemical compositional analysis using XPS and ATR FT-IR confirms the incorporation of aldehyde functionality after functionalization, which is corroborated by surface energy calculations following the Van Oss-Chaudhury-Good method.					
35775198	8	75	theme	Cytoskeletal	1947:1958	arg1	assessment					1973:1982	Cytoskeletal architecture assessment	1947:1982	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF)	1947:2110	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	4	76	theme	Van	1170:1172	arg1	method					1193:1198	the Van Oss-Chaudhury-Good method	1166:1198	the Van Oss-Chaudhury-Good method	1166:1198	Surface chemical compositional analysis using XPS and ATR FT-IR confirms the incorporation of aldehyde functionality after functionalization, which is corroborated by surface energy calculations following the Van Oss-Chaudhury-Good method.					
35775198	10	77	theme	nanocomposites	2519:2532	arg1	potential					2535:2543	the functionalized nanocomposites' potential	2500:2543	the functionalized nanocomposites' potential	2500:2543	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	3	78	theme	Ch	701:702	arg1	matrix					704:709	Ch matrix	701:709	Ch matrix	701:709	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	8	79	theme	growth	2057:2062	arg1	bFGF					2072:2075	bFGF	2072:2075	bFGF	2072:2075	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	8	79	theme	growth	2057:2062	arg1	factor					2064:2069	each basic fibroblast growth factor	2035:2069	each basic fibroblast growth factor (bFGF)	2035:2076	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	9	80	theme	cells	2284:2288	arg1	%					2279:2279	more than 85%	2267:2279	more than 85% of cells	2267:2288	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	9	80	theme	cells	2284:2288	arg1	cells					2284:2288	cells	2284:2288	cells	2284:2288	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	6	81	theme	enzyme	1522:1527	arg1	kinetics					1529:1536	better enzyme kinetics	1515:1536	better enzyme kinetics	1515:1536	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	4	82	theme	energy	1136:1141	arg1	calculations					1143:1154	surface energy calculations	1128:1154	surface energy calculations following the Van Oss-Chaudhury-Good method	1128:1198	Surface chemical compositional analysis using XPS and ATR FT-IR confirms the incorporation of aldehyde functionality after functionalization, which is corroborated by surface energy calculations following the Van Oss-Chaudhury-Good method.					
35775198	3	83	theme	optimal	717:723	arg1	concentration					725:737	an optimal concentration	714:737	an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test	714:958	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	3	83	theme	optimal	717:723	arg1	dispersion					687:696	4 wt % PNFs dispersion	675:696	4 wt % PNFs dispersion in Ch matrix	675:709	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	5	84	theme	energy	1316:1321	arg1	component					1295:1303	the polar component	1285:1303	the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1	1285:1353	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	7	85	theme	cells	1891:1895	arg1	adhesion					1823:1830	adhesion	1823:1830	adhesion	1823:1830	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	7	85	theme	cells	1891:1895	arg1	spreading					1837:1845	spreading	1837:1845	spreading	1837:1845	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	7	85	theme	cells	1891:1895	arg1	viability					1812:1820	improved viability	1803:1820	improved viability	1803:1820	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	5	86	from	m-1	1351:1353	arg1	energy					1316:1321	surface energy	1308:1321	surface energy (γAB) from 6.35 to 12.54 mN m-1	1308:1353	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	5	86	from	m-1	1351:1353	arg1	γAB					1324:1326	γAB	1324:1326	γAB	1324:1326	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	5	86	from	m-1	1351:1353	arg1	component					1295:1303	the polar component	1285:1303	the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1	1285:1353	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	2	87	theme	dispersed	473:481	arg1	scaffold					512:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold	430:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	430:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	7	88	theme	mesenchymal	1874:1884	arg1	ADMSCs					1898:1903	ADMSCs	1898:1903	ADMSCs	1898:1903	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	7	88	theme	mesenchymal	1874:1884	arg1	cells					1891:1895	primary adipose derived mesenchymal stem cells	1850:1895	primary adipose derived mesenchymal stem cells (ADMSCs)	1850:1904	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	3	89	theme	desirable	753:761	arg1	functions					774:782	desirable biological functions	753:782	desirable biological functions	753:782	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	8	90	contain	containing	2012:2021	arg1	media					2006:2010	differentiating media	1990:2010	differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF)	1990:2110	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	8	90	contain	containing	2012:2021	arg2	ng/mL					2026:2030	10 ng/mL	2023:2030	10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF)	2023:2110	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	7	91	theme	adipose	1858:1864	arg1	ADMSCs					1898:1903	ADMSCs	1898:1903	ADMSCs	1898:1903	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	7	91	theme	adipose	1858:1864	arg1	cells					1891:1895	primary adipose derived mesenchymal stem cells	1850:1895	primary adipose derived mesenchymal stem cells (ADMSCs)	1850:1904	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	5	92	from	increase	1369:1376	arg1	polarity					1389:1396	surface polarity	1381:1396	surface polarity from 13.61 to 22.54%	1381:1417	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	5	92	from	increase	1369:1376	arg1	%					1417:1417	13.61 to 22.54%	1403:1417	13.61 to 22.54%	1403:1417	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	3	93	theme	physicochemical	811:825	arg1	properties					827:836	required physicochemical properties	802:836	required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test	802:958	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	0	94	theme	Cells	157:161	arg1	Differentiation					97:111	Neuronal-like Differentiation	83:111	Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells	83:161	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	0	94	theme	Cells	157:161	arg1	Activity					174:181	Urease Activity	167:181	Urease Activity	167:181	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	4	95	theme	Surface	961:967	arg1	analysis					992:999	Surface chemical compositional analysis	961:999	Surface chemical compositional analysis using XPS and ATR FT-IR	961:1023	Surface chemical compositional analysis using XPS and ATR FT-IR confirms the incorporation of aldehyde functionality after functionalization, which is corroborated by surface energy calculations following the Van Oss-Chaudhury-Good method.					
35775198	9	96	theme	specific	2313:2320	arg1	protein					2336:2342	early neuron specific β III tubulin protein	2300:2342	early neuron specific β III tubulin protein on the functionalized scaffolds	2300:2374	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	1	97	from	engineering	287:297	arg1	relevant					268:275	relevant	268:275	relevant	268:275	Bioscaffolds having electrically conducting polymers (CPs) have become increasingly relevant in tissue engineering (TE) because of their ability to regulate conductivity and promote biological function.					
35775198	10	98	dep	flexible	2626:2633	arg1	designed					2648:2655	designed	2648:2655	to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves	2642:2780	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	6	99	theme	Michaelis	1558:1566	arg1	Km					1579:1580	Km	1579:1580	Km	1579:1580	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	6	99	theme	Michaelis	1558:1566	arg1	constants					1568:1576	Michaelis constants	1558:1576	Michaelis constants (Km)	1558:1581	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	2	100	theme	functionalization	552:568	arg1	approach					570:577	a simple one-step surface functionalization approach	526:577	a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	526:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	9	101	theme	early	2300:2304	arg1	protein					2336:2342	early neuron specific β III tubulin protein	2300:2342	early neuron specific β III tubulin protein on the functionalized scaffolds	2300:2374	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	2	102	theme	neural	614:619	arg1	applications					634:645	potential neural regeneration applications	604:645	potential neural regeneration applications	604:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	7	103	theme	derived	1866:1872	arg1	ADMSCs					1898:1903	ADMSCs	1898:1903	ADMSCs	1898:1903	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	7	103	theme	derived	1866:1872	arg1	cells					1891:1895	primary adipose derived mesenchymal stem cells	1850:1895	primary adipose derived mesenchymal stem cells (ADMSCs)	1850:1904	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	6	104	theme	favorable	1618:1626	arg1	interactions					1648:1659	favorable protein-biomaterial interactions	1618:1659	favorable protein-biomaterial interactions	1618:1659	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	3	105	theme	strength	910:917	arg1	test					919:922	mechanical strength test	899:922	mechanical strength test	899:922	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	5	106	from	%	1417:1417	arg1	polarity					1389:1396	surface polarity	1381:1396	surface polarity from 13.61 to 22.54%	1381:1417	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	5	106	from	%	1417:1417	arg1	increase					1369:1376	an increase	1366:1376	an increase in surface polarity from 13.61 to 22.54%	1366:1417	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	0	107	theme	Neuronal-like	83:95	arg1	Differentiation					97:111	Neuronal-like Differentiation	83:111	Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells	83:161	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	6	108	from	mM	1603:1604	arg1	reduction					1545:1553	a reduction	1543:1553	a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM	1543:1604	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	9	109	theme	glial	2385:2389	arg1	GFAP					2418:2421	GFAP	2418:2421	GFAP	2418:2421	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	9	109	theme	glial	2385:2389	arg1	protein					2409:2415	glial fibrillary acidic protein	2385:2415	glial fibrillary acidic protein (GFAP) expression	2385:2433	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	8	110	theme	factor	2099:2104	arg1	ng/mL					2026:2030	10 ng/mL	2023:2030	10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF)	2023:2110	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	1	111	theme	biological	366:375	arg1	function					377:384	biological function	366:384	biological function	366:384	Bioscaffolds having electrically conducting polymers (CPs) have become increasingly relevant in tissue engineering (TE) because of their ability to regulate conductivity and promote biological function.					
35775198	6	112	dep	94	1507:1508	arg1	to					1504:1505	to	1504:1505	to	1504:1505	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	3	113	theme	in	929:930	arg1	test					955:958	in vitro biodegradability test	929:958	in vitro biodegradability test	929:958	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	5	114	theme	mN	1348:1349	arg1	m-1					1351:1353	6.35 to 12.54 mN m-1	1334:1353	6.35 to 12.54 mN m-1	1334:1353	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	0	115	theme	Adipose	124:130	arg1	Cells					157:161	Primary Adipose Derived Mesenchymal Stem Cells	116:161	Primary Adipose Derived Mesenchymal Stem Cells	116:161	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	2	116	theme	one-step	535:542	arg1	approach					570:577	a simple one-step surface functionalization approach	526:577	a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	526:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	9	117	theme	fibrillary	2391:2400	arg1	GFAP					2418:2421	GFAP	2418:2421	GFAP	2418:2421	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	9	117	theme	fibrillary	2391:2400	arg1	protein					2409:2415	glial fibrillary acidic protein	2385:2415	glial fibrillary acidic protein (GFAP) expression	2385:2433	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	6	118	theme	Functionalized	1420:1433	arg1	PNF					1435:1437	Functionalized PNF	1420:1437	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.	1420:1720	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	2	119	with	scaffold	512:519	arg1	approach					570:577	a simple one-step surface functionalization approach	526:577	a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	526:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	7	120	theme	improved	1803:1810	arg1	viability					1812:1820	improved viability	1803:1820	improved viability	1803:1820	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	10	121	theme	functionalized	2504:2517	arg1	potential					2535:2543	the functionalized nanocomposites' potential	2500:2543	the functionalized nanocomposites' potential	2500:2543	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	0	122	theme	Mesenchymal	140:150	arg1	Cells					157:161	Primary Adipose Derived Mesenchymal Stem Cells	116:161	Primary Adipose Derived Mesenchymal Stem Cells	116:161	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	0	123	theme	Functionalized	8:21	arg1	Nanofibers					35:44	Surface Functionalized Polyaniline Nanofibers	0:44	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.	0:182	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	5	124	theme	Surface	1201:1207	arg1	functionalization					1209:1225	Surface functionalization	1201:1225	Surface functionalization	1201:1225	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	3	125	theme	biodegradability	938:953	arg1	test					955:958	in vitro biodegradability test	929:958	in vitro biodegradability test	929:958	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	2	126	theme	PNFs	467:470	arg1	scaffold					512:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold	430:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	430:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	6	127	theme	perceptible	1699:1709	arg1	materials					1711:1719	biologically perceptible materials	1686:1719	biologically perceptible materials	1686:1719	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	6	127	theme	perceptible	1699:1709	arg1	them					1678:1681	them	1678:1681	them	1678:1681	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	7	128	theme	cell-biomaterial	1766:1781	arg1	interactions					1783:1794	improved cell-biomaterial interactions	1757:1794	improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility	1757:1944	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	2	129	theme	nanofibers	455:464	arg1	scaffold					512:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold	430:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	430:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	3	130	theme	PNFs	682:685	arg1	concentration					725:737	an optimal concentration	714:737	an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test	714:958	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	3	130	theme	PNFs	682:685	arg1	dispersion					687:696	4 wt % PNFs dispersion	675:696	4 wt % PNFs dispersion in Ch matrix	675:709	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	10	131	theme	mechanical	2739:2748	arg1	properties					2750:2759	mechanical properties	2739:2759	mechanical properties	2739:2759	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	2	132	theme	conducting	432:441	arg1	scaffold					512:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold	430:519	a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications	430:645	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	5	133	from	enhancement	1235:1245	arg1	hydrophilicity					1258:1271	surface hydrophilicity	1250:1271	surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1	1250:1353	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	5	134	theme	component	1295:1303	arg1	terms					1276:1280	terms	1276:1280	terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1	1276:1353	Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γAB) from 6.35 to 12.54 mN m-1 along with an increase in surface polarity from 13.61 to 22.54%.					
35775198	8	135	theme	basic	2040:2044	arg1	bFGF					2072:2075	bFGF	2072:2075	bFGF	2072:2075	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	8	135	theme	basic	2040:2044	arg1	factor					2064:2069	each basic fibroblast growth factor	2035:2069	each basic fibroblast growth factor (bFGF)	2035:2076	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	10	136	theme	stimulated	2582:2591	arg1	regeneration					2600:2611	electrically stimulated neural regeneration	2569:2611	electrically stimulated neural regeneration	2569:2611	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	3	137	theme	wt	677:678	arg1	concentration					725:737	an optimal concentration	714:737	an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test	714:958	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	3	137	theme	wt	677:678	arg1	dispersion					687:696	4 wt % PNFs dispersion	675:696	4 wt % PNFs dispersion in Ch matrix	675:709	According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current-voltage (I-V) measurement, mechanical strength test, and in vitro biodegradability test.					
35775198	6	138	theme	enzyme	1480:1485	arg1	activity					1487:1494	enzyme activity	1480:1494	enzyme activity from 67 to 94%	1480:1509	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	4	139	theme	functionality	1064:1076	arg1	incorporation					1038:1050	the incorporation	1034:1050	the incorporation of aldehyde functionality	1034:1076	Surface chemical compositional analysis using XPS and ATR FT-IR confirms the incorporation of aldehyde functionality after functionalization, which is corroborated by surface energy calculations following the Van Oss-Chaudhury-Good method.					
35775198	10	140	theme	nerves	2775:2780	arg1	microstructures					2719:2733	microstructures	2719:2733	microstructures	2719:2733	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	10	140	theme	nerves	2775:2780	arg1	properties					2750:2759	mechanical properties	2739:2759	mechanical properties	2739:2759	The findings point to the functionalized nanocomposites' potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.					
35775198	9	141	theme	protein	2409:2415	arg1	expression					2424:2433	glial fibrillary acidic protein (GFAP) expression	2385:2433	glial fibrillary acidic protein (GFAP) expression	2385:2433	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	0	142	dep	Nanofibers	35:44	arg1	Nanocomposite					55:67	Chitosan Nanocomposite	46:67	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.	0:182	Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity.					
35775198	9	143	theme	Immunocytochemistry	2227:2245	arg1	results					2247:2253	Immunocytochemistry results	2227:2253	Immunocytochemistry results	2227:2253	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	9	144	theme	cells	2471:2475	arg1	cells					2471:2475	cells	2471:2475	cells	2471:2475	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	9	144	theme	cells	2471:2475	arg1	%					2466:2466	approximately 40%	2450:2466	approximately 40% of cells	2450:2475	Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells.					
35775198	7	145	link	derived	1866:1872	arg1	ADMSCs					1898:1903	ADMSCs	1898:1903	ADMSCs	1898:1903	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	7	145	link	derived	1866:1872	arg1	cells					1891:1895	primary adipose derived mesenchymal stem cells	1850:1895	primary adipose derived mesenchymal stem cells (ADMSCs)	1850:1904	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	6	146	from	kinetics	1529:1536	arg1	activity					1487:1494	enzyme activity	1480:1494	enzyme activity from 67 to 94%	1480:1509	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	6	146	from	kinetics	1529:1536	arg1	%					1509:1509	67 to 94%	1501:1509	67 to 94%	1501:1509	Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (Km) from 21.55 to 13.81 mM, indicating favorable protein-biomaterial interactions and establishing them as biologically perceptible materials.					
35775198	7	147	theme	improved	1917:1924	arg1	immunocompatibility					1926:1944	improved immunocompatibility	1917:1944	improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility	1757:1944	Surface functionalization mediated improved cell-biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility.					
35775198	2	148	theme	current	410:416	arg1	study					418:422	the current study	406:422	the current study	406:422	With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications.					
35775198	8	149	theme	actin	2133:2137	arg1	remodeling					2139:2148	significant actin remodeling	2121:2148	significant actin remodeling with neurite-like projections	2121:2178	Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days.					
35775198	1	150	theme	tissue	280:285	arg1	TE					300:301	TE	300:301	TE	300:301	Bioscaffolds having electrically conducting polymers (CPs) have become increasingly relevant in tissue engineering (TE) because of their ability to regulate conductivity and promote biological function.					
35775198	1	150	theme	tissue	280:285	arg1	engineering					287:297	tissue engineering	280:297	tissue engineering (TE)	280:302	Bioscaffolds having electrically conducting polymers (CPs) have become increasingly relevant in tissue engineering (TE) because of their ability to regulate conductivity and promote biological function.					
36101011	4	0	theme	milling	524:530	arg1	methods					446:452	three different methods	430:452	three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM)	430:535	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	11	1	theme	mill	1228:1231	arg1	flour					1233:1237	pin mill flour	1224:1237	pin mill flour to have a higher peak viscosity (PV) (2,295 cP)	1224:1285	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	11	2	theme	peak	1256:1259	arg1	PV					1272:1273	PV	1272:1273	PV	1272:1273	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	11	2	theme	peak	1256:1259	arg1	2,295 cP					1277:1284	2,295 cP	1277:1284	2,295 cP	1277:1284	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	11	2	theme	peak	1256:1259	arg1	viscosity					1261:1269	a higher peak viscosity	1247:1269	a higher peak viscosity (PV) (2,295 cP)	1247:1285	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	9	3	theme	nutritional	1019:1029	arg1	composition					1031:1041	The nutritional composition	1015:1041	The nutritional composition	1015:1041	The nutritional composition was not significantly affected by different grinding methods.					
36101011	6	4	theme	size	718:721	arg1	distribution					723:734	The particle size distribution	705:734	The particle size distribution of all the flours	705:752	The particle size distribution of all the flours showed bi-modal distribution except for the RM flour.					
36101011	13	5	theme	loaves	1536:1541	arg1	volume					1520:1525	the specific volume	1507:1525	the specific volume of bread loaves and C-cell characteristics	1507:1568	The grinding method did not affect the specific volume of bread loaves and C-cell characteristics.					
36101011	15	6	theme	millet	1704:1709	arg1	products					1716:1723	value-added proso millet food products	1686:1723	value-added proso millet food products	1686:1723	This study will help in promoting and producing value-added proso millet food products with enhanced nutritional quality.					
36101011	2	7	theme	needed	287:292	arg1	alternatives					302:313	much needed healthy alternatives	282:313	much needed healthy alternatives	282:313	They can be incorporated into the grain industry and provide much needed healthy alternatives.					
36101011	1	8	theme	gluten	170:175	arg1	food					182:185	a nutritious, sustainable, and gluten free food	139:185	a nutritious, sustainable, and gluten free food which is currently underutilized	139:218	Proso millet is a nutritious, sustainable, and gluten free food which is currently underutilized.					
36101011	1	8	theme	gluten	170:175	arg1	millet					129:134	Proso millet	123:134	Proso millet	123:134	Proso millet is a nutritious, sustainable, and gluten free food which is currently underutilized.					
36101011	12	9	from	flour	1418:1422	arg1	possible					1462:1469	possible	1462:1469	possible	1462:1469	Finally, this study demonstrated that the production of bread from proso millet flour with desirable quality and texture is possible.					
36101011	12	9	from	flour	1418:1422	arg1	production					1380:1389	the production	1376:1389	the production of bread from proso millet flour with desirable quality and texture	1376:1457	Finally, this study demonstrated that the production of bread from proso millet flour with desirable quality and texture is possible.					
36101011	13	10	theme	C-cell	1547:1552	arg1	characteristics					1554:1568	C-cell characteristics	1547:1568	C-cell characteristics	1547:1568	The grinding method did not affect the specific volume of bread loaves and C-cell characteristics.					
36101011	8	11	from	damage	935:940	arg1	flour					952:956	the PM flour	945:956	the PM flour (4.64%)	945:964	The study also revealed that starch damage in the PM flour (4.64%) was higher than RM (2.46%) and HM flour (2.51%).					
36101011	8	11	from	damage	935:940	arg1	%					963:963	4.64%	959:963	4.64%	959:963	The study also revealed that starch damage in the PM flour (4.64%) was higher than RM (2.46%) and HM flour (2.51%).					
36101011	4	12	theme	baking	572:577	arg1	properties					579:588	baking properties	572:588	baking properties	572:588	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	13	13	theme	specific	1511:1518	arg1	volume					1520:1525	the specific volume	1507:1525	the specific volume of bread loaves and C-cell characteristics	1507:1568	The grinding method did not affect the specific volume of bread loaves and C-cell characteristics.					
36101011	9	14	theme	grinding	1087:1094	arg1	methods					1096:1102	different grinding methods	1077:1102	different grinding methods	1077:1102	The nutritional composition was not significantly affected by different grinding methods.					
36101011	13	15	theme	characteristics	1554:1568	arg1	volume					1520:1525	the specific volume	1507:1525	the specific volume of bread loaves and C-cell characteristics	1507:1568	The grinding method did not affect the specific volume of bread loaves and C-cell characteristics.					
36101011	4	16	theme	methods	446:452	arg1	effect					420:425	the effect	416:425	the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application	416:609	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	11	17	theme	Analysis	1200:1207	arg1	profile					1209:1215	Rapid Visco Analysis profile	1188:1215	Rapid Visco Analysis profile	1188:1215	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	4	18	from	effect	420:425	arg1	rheology					559:566	proso millet flour rheology	540:566	proso millet flour rheology	540:566	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	4	18	from	effect	420:425	arg1	properties					579:588	baking properties	572:588	baking properties	572:588	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	5	19	theme	flow	624:627	arg1	sheet					629:633	The milling flow sheet	612:633	The milling flow sheet	612:633	The milling flow sheet was developed toward the production of the quality whole grain flour.					
36101011	3	20	theme	Efficient	316:324	arg1	method					335:340	Efficient grinding method	316:340	Efficient grinding method	316:340	Efficient grinding method should be adopted for easy incorporation.					
36101011	5	21	theme	whole	686:690	arg1	flour					698:702	the quality whole grain flour	674:702	the quality whole grain flour	674:702	The milling flow sheet was developed toward the production of the quality whole grain flour.					
36101011	7	22	theme	82 μm	892:896	arg1	diameter					880:887	geometric mean diameter	865:887	geometric mean diameter of 82 μm	865:896	The PM produced the flour with the finest particles with geometric mean diameter of 82 μm.					
36101011	4	23	theme	milling	481:487	arg1	methods					446:452	three different methods	430:452	three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM)	430:535	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	15	24	theme	nutritional	1739:1749	arg1	quality					1751:1757	enhanced nutritional quality	1730:1757	enhanced nutritional quality	1730:1757	This study will help in promoting and producing value-added proso millet food products with enhanced nutritional quality.					
36101011	0	25	theme	physicochemical	70:84	arg1	properties					111:120	physicochemical, rheological, and baking properties	70:120	physicochemical, rheological, and baking properties	70:120	Significance of different milling methods on white proso millet flour physicochemical, rheological, and baking properties.					
36101011	0	26	from	Significance	0:11	arg1	millet					57:62	white proso millet	45:62	white proso millet	45:62	Significance of different milling methods on white proso millet flour physicochemical, rheological, and baking properties.					
36101011	0	27	theme	rheological	87:97	arg1	properties					111:120	physicochemical, rheological, and baking properties	70:120	physicochemical, rheological, and baking properties	70:120	Significance of different milling methods on white proso millet flour physicochemical, rheological, and baking properties.					
36101011	7	28	theme	geometric	865:873	arg1	diameter					880:887	geometric mean diameter	865:887	geometric mean diameter of 82 μm	865:896	The PM produced the flour with the finest particles with geometric mean diameter of 82 μm.					
36101011	4	29	theme	grinding	457:464	arg1	RM					490:491	RM	490:491	RM	490:491	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	4	29	theme	grinding	457:464	arg1	milling					481:487	roller milling	474:487	roller milling (RM)	474:492	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	15	30	theme	food	1711:1714	arg1	products					1716:1723	value-added proso millet food products	1686:1723	value-added proso millet food products	1686:1723	This study will help in promoting and producing value-added proso millet food products with enhanced nutritional quality.					
36101011	15	31	theme	proso	1698:1702	arg1	products					1716:1723	value-added proso millet food products	1686:1723	value-added proso millet food products	1686:1723	This study will help in promoting and producing value-added proso millet food products with enhanced nutritional quality.					
36101011	14	32	theme	breads	1598:1603	arg1	volume					1584:1589	The specific volume	1571:1589	The specific volume of the breads	1571:1603	The specific volume of the breads ranged from 2.40 to 2.52 cm3 /g.					
36101011	0	33	theme	different	16:24	arg1	methods					34:40	different milling methods	16:40	different milling methods	16:40	Significance of different milling methods on white proso millet flour physicochemical, rheological, and baking properties.					
36101011	6	34	theme	RM	798:799	arg1	flour					801:805	the RM flour	794:805	the RM flour	794:805	The particle size distribution of all the flours showed bi-modal distribution except for the RM flour.					
36101011	12	35	theme	desirable	1429:1437	arg1	quality					1439:1445	desirable quality	1429:1445	desirable quality	1429:1445	Finally, this study demonstrated that the production of bread from proso millet flour with desirable quality and texture is possible.					
36101011	0	36	theme	methods	34:40	arg1	Significance					0:11	Significance	0:11	Significance of different milling methods on white proso millet	0:62	Significance of different milling methods on white proso millet flour physicochemical, rheological, and baking properties.					
36101011	14	37	theme	specific	1575:1582	arg1	volume					1584:1589	The specific volume	1571:1589	The specific volume of the breads	1571:1603	The specific volume of the breads ranged from 2.40 to 2.52 cm3 /g.					
36101011	0	38	theme	white	45:49	arg1	millet					57:62	white proso millet	45:62	white proso millet	45:62	Significance of different milling methods on white proso millet flour physicochemical, rheological, and baking properties.					
36101011	15	39	theme	value-added	1686:1696	arg1	products					1716:1723	value-added proso millet food products	1686:1723	value-added proso millet food products	1686:1723	This study will help in promoting and producing value-added proso millet food products with enhanced nutritional quality.					
36101011	4	40	theme	milling	499:505	arg1	methods					446:452	three different methods	430:452	three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM)	430:535	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	4	41	theme	proso	540:544	arg1	rheology					559:566	proso millet flour rheology	540:566	proso millet flour rheology	540:566	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	11	42	theme	pin	1224:1226	arg1	flour					1233:1237	pin mill flour	1224:1237	pin mill flour to have a higher peak viscosity (PV) (2,295 cP)	1224:1285	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	8	43	theme	HM	997:998	arg1	flour					1000:1004	HM flour	997:1004	HM flour (2.51%)	997:1012	The study also revealed that starch damage in the PM flour (4.64%) was higher than RM (2.46%) and HM flour (2.51%).					
36101011	8	43	theme	HM	997:998	arg1	%					1011:1011	2.51%	1007:1011	2.51%	1007:1011	The study also revealed that starch damage in the PM flour (4.64%) was higher than RM (2.46%) and HM flour (2.51%).					
36101011	4	44	theme	hammer	517:522	arg1	HM					533:534	HM	533:534	HM	533:534	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	4	44	theme	hammer	517:522	arg1	milling					524:530	hammer milling	517:530	hammer milling (HM)	517:535	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	11	45	theme	higher	1249:1254	arg1	PV					1272:1273	PV	1272:1273	PV	1272:1273	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	11	45	theme	higher	1249:1254	arg1	2,295 cP					1277:1284	2,295 cP	1277:1284	2,295 cP	1277:1284	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	11	45	theme	higher	1249:1254	arg1	viscosity					1261:1269	a higher peak viscosity	1247:1269	a higher peak viscosity (PV) (2,295 cP)	1247:1285	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	2	46	theme	grain	255:259	arg1	industry					261:268	the grain industry	251:268	the grain industry	251:268	They can be incorporated into the grain industry and provide much needed healthy alternatives.					
36101011	1	47	theme	sustainable	153:163	arg1	food					182:185	a nutritious, sustainable, and gluten free food	139:185	a nutritious, sustainable, and gluten free food which is currently underutilized	139:218	Proso millet is a nutritious, sustainable, and gluten free food which is currently underutilized.					
36101011	1	47	theme	sustainable	153:163	arg1	millet					129:134	Proso millet	123:134	Proso millet	123:134	Proso millet is a nutritious, sustainable, and gluten free food which is currently underutilized.					
36101011	7	48	with	flour	828:832	arg1	particles					850:858	the finest particles	839:858	the finest particles	839:858	The PM produced the flour with the finest particles with geometric mean diameter of 82 μm.					
36101011	7	48	with	flour	828:832	arg1	diameter					880:887	geometric mean diameter	865:887	geometric mean diameter of 82 μm	865:896	The PM produced the flour with the finest particles with geometric mean diameter of 82 μm.					
36101011	11	49	theme	Rapid	1188:1192	arg1	profile					1209:1215	Rapid Visco Analysis profile	1188:1215	Rapid Visco Analysis profile	1188:1215	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	13	50	theme	bread	1530:1534	arg1	loaves					1536:1541	bread loaves	1530:1541	bread loaves	1530:1541	The grinding method did not affect the specific volume of bread loaves and C-cell characteristics.					
36101011	5	51	theme	flour	698:702	arg1	production					660:669	the production	656:669	the production of the quality whole grain flour	656:702	The milling flow sheet was developed toward the production of the quality whole grain flour.					
36101011	6	52	theme	particle	709:716	arg1	distribution					723:734	The particle size distribution	705:734	The particle size distribution of all the flours	705:752	The particle size distribution of all the flours showed bi-modal distribution except for the RM flour.					
36101011	2	53	theme	healthy	294:300	arg1	alternatives					302:313	much needed healthy alternatives	282:313	much needed healthy alternatives	282:313	They can be incorporated into the grain industry and provide much needed healthy alternatives.					
36101011	12	54	theme	bread	1394:1398	arg1	possible					1462:1469	possible	1462:1469	possible	1462:1469	Finally, this study demonstrated that the production of bread from proso millet flour with desirable quality and texture is possible.					
36101011	12	54	theme	bread	1394:1398	arg1	production					1380:1389	the production	1376:1389	the production of bread from proso millet flour with desirable quality and texture	1376:1457	Finally, this study demonstrated that the production of bread from proso millet flour with desirable quality and texture is possible.					
36101011	11	55	dep	showed	1217:1222	arg1	compared					1287:1294	compared	1287:1294	showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP)	1217:1335	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	8	56	theme	starch	928:933	arg1	damage					935:940	starch damage	928:940	starch damage in the PM flour (4.64%)	928:964	The study also revealed that starch damage in the PM flour (4.64%) was higher than RM (2.46%) and HM flour (2.51%).					
36101011	4	57	theme	food	594:597	arg1	application					599:609	food application	594:609	food application	594:609	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	1	58	theme	free	177:180	arg1	food					182:185	a nutritious, sustainable, and gluten free food	139:185	a nutritious, sustainable, and gluten free food which is currently underutilized	139:218	Proso millet is a nutritious, sustainable, and gluten free food which is currently underutilized.					
36101011	1	58	theme	free	177:180	arg1	millet					129:134	Proso millet	123:134	Proso millet	123:134	Proso millet is a nutritious, sustainable, and gluten free food which is currently underutilized.					
36101011	12	59	theme	proso	1405:1409	arg1	flour					1418:1422	proso millet flour	1405:1422	proso millet flour with desirable quality and texture	1405:1457	Finally, this study demonstrated that the production of bread from proso millet flour with desirable quality and texture is possible.					
36101011	4	60	theme	different	436:444	arg1	methods					446:452	three different methods	430:452	three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM)	430:535	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	4	61	theme	flour	553:557	arg1	rheology					559:566	proso millet flour rheology	540:566	proso millet flour rheology	540:566	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	9	62	theme	different	1077:1085	arg1	methods					1096:1102	different grinding methods	1077:1102	different grinding methods	1077:1102	The nutritional composition was not significantly affected by different grinding methods.					
36101011	11	63	theme	Visco	1194:1198	arg1	profile					1209:1215	Rapid Visco Analysis profile	1188:1215	Rapid Visco Analysis profile	1188:1215	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	5	64	theme	milling	616:622	arg1	sheet					629:633	The milling flow sheet	612:633	The milling flow sheet	612:633	The milling flow sheet was developed toward the production of the quality whole grain flour.					
36101011	1	65	theme	Proso	123:127	arg1	food					182:185	a nutritious, sustainable, and gluten free food	139:185	a nutritious, sustainable, and gluten free food which is currently underutilized	139:218	Proso millet is a nutritious, sustainable, and gluten free food which is currently underutilized.					
36101011	1	65	theme	Proso	123:127	arg1	millet					129:134	Proso millet	123:134	Proso millet	123:134	Proso millet is a nutritious, sustainable, and gluten free food which is currently underutilized.					
36101011	5	66	theme	quality	678:684	arg1	flour					698:702	the quality whole grain flour	674:702	the quality whole grain flour	674:702	The milling flow sheet was developed toward the production of the quality whole grain flour.					
36101011	3	67	theme	grinding	326:333	arg1	method					335:340	Efficient grinding method	316:340	Efficient grinding method	316:340	Efficient grinding method should be adopted for easy incorporation.					
36101011	11	68	theme	RM	1317:1318	arg1	2,130 cP					1327:1334	2,130 cP	1327:1334	2,130 cP	1327:1334	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	11	68	theme	RM	1317:1318	arg1	flour					1320:1324	RM flour	1317:1324	RM flour (2,130 cP)	1317:1335	Rapid Visco Analysis profile showed pin mill flour to have a higher peak viscosity (PV) (2,295 cP) compared to HM (2,065 cP) and RM flour (2,130 cP).					
36101011	5	69	theme	grain	692:696	arg1	flour					698:702	the quality whole grain flour	674:702	the quality whole grain flour	674:702	The milling flow sheet was developed toward the production of the quality whole grain flour.					
36101011	1	70	theme	nutritious	141:150	arg1	food					182:185	a nutritious, sustainable, and gluten free food	139:185	a nutritious, sustainable, and gluten free food which is currently underutilized	139:218	Proso millet is a nutritious, sustainable, and gluten free food which is currently underutilized.					
36101011	1	70	theme	nutritious	141:150	arg1	millet					129:134	Proso millet	123:134	Proso millet	123:134	Proso millet is a nutritious, sustainable, and gluten free food which is currently underutilized.					
36101011	10	71	theme	Pasting	1105:1111	arg1	properties					1113:1122	Pasting properties	1105:1122	Pasting properties of the flour	1105:1135	Pasting properties of the flour were also affected by the grinding method applied.					
36101011	8	72	theme	PM	949:950	arg1	flour					952:956	the PM flour	945:956	the PM flour (4.64%)	945:964	The study also revealed that starch damage in the PM flour (4.64%) was higher than RM (2.46%) and HM flour (2.51%).					
36101011	8	72	theme	PM	949:950	arg1	%					963:963	4.64%	959:963	4.64%	959:963	The study also revealed that starch damage in the PM flour (4.64%) was higher than RM (2.46%) and HM flour (2.51%).					
36101011	15	73	theme	enhanced	1730:1737	arg1	quality					1751:1757	enhanced nutritional quality	1730:1757	enhanced nutritional quality	1730:1757	This study will help in promoting and producing value-added proso millet food products with enhanced nutritional quality.					
36101011	0	74	theme	baking	104:109	arg1	properties					111:120	physicochemical, rheological, and baking properties	70:120	physicochemical, rheological, and baking properties	70:120	Significance of different milling methods on white proso millet flour physicochemical, rheological, and baking properties.					
36101011	7	75	theme	mean	875:878	arg1	diameter					880:887	geometric mean diameter	865:887	geometric mean diameter of 82 μm	865:896	The PM produced the flour with the finest particles with geometric mean diameter of 82 μm.					
36101011	4	76	theme	roller	474:479	arg1	RM					490:491	RM	490:491	RM	490:491	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	4	76	theme	roller	474:479	arg1	milling					481:487	roller milling	474:487	roller milling (RM)	474:492	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	10	77	theme	flour	1131:1135	arg1	properties					1113:1122	Pasting properties	1105:1122	Pasting properties of the flour	1105:1135	Pasting properties of the flour were also affected by the grinding method applied.					
36101011	13	78	theme	grinding	1476:1483	arg1	method					1485:1490	The grinding method	1472:1490	The grinding method	1472:1490	The grinding method did not affect the specific volume of bread loaves and C-cell characteristics.					
36101011	10	79	theme	grinding	1163:1170	arg1	method					1172:1177	the grinding method	1159:1177	the grinding method applied	1159:1185	Pasting properties of the flour were also affected by the grinding method applied.					
36101011	0	80	theme	milling	26:32	arg1	methods					34:40	different milling methods	16:40	different milling methods	16:40	Significance of different milling methods on white proso millet flour physicochemical, rheological, and baking properties.					
36101011	7	81	theme	finest	843:848	arg1	particles					850:858	the finest particles	839:858	the finest particles	839:858	The PM produced the flour with the finest particles with geometric mean diameter of 82 μm.					
36101011	12	82	theme	millet	1411:1416	arg1	flour					1418:1422	proso millet flour	1405:1422	proso millet flour with desirable quality and texture	1405:1457	Finally, this study demonstrated that the production of bread from proso millet flour with desirable quality and texture is possible.					
36101011	3	83	theme	easy	364:367	arg1	incorporation					369:381	easy incorporation	364:381	easy incorporation	364:381	Efficient grinding method should be adopted for easy incorporation.					
36101011	4	84	theme	pin	495:497	arg1	milling					499:505	pin milling	495:505	pin milling (PM)	495:510	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	4	84	theme	pin	495:497	arg1	PM					508:509	PM	508:509	PM	508:509	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
36101011	0	85	theme	proso	51:55	arg1	millet					57:62	white proso millet	45:62	white proso millet	45:62	Significance of different milling methods on white proso millet flour physicochemical, rheological, and baking properties.					
36101011	6	86	theme	bi-modal	761:768	arg1	distribution					770:781	bi-modal distribution	761:781	bi-modal distribution	761:781	The particle size distribution of all the flours showed bi-modal distribution except for the RM flour.					
36101011	6	87	theme	flours	747:752	arg1	distribution					723:734	The particle size distribution	705:734	The particle size distribution of all the flours	705:752	The particle size distribution of all the flours showed bi-modal distribution except for the RM flour.					
36101011	12	88	with	flour	1418:1422	arg1	texture					1451:1457	texture	1451:1457	texture	1451:1457	Finally, this study demonstrated that the production of bread from proso millet flour with desirable quality and texture is possible.					
36101011	12	88	with	flour	1418:1422	arg1	quality					1439:1445	desirable quality	1429:1445	desirable quality	1429:1445	Finally, this study demonstrated that the production of bread from proso millet flour with desirable quality and texture is possible.					
36101011	4	89	theme	millet	546:551	arg1	rheology					559:566	proso millet flour rheology	540:566	proso millet flour rheology	540:566	This study aimed to investigate the effect of three different methods of grinding namely, roller milling (RM), pin milling (PM), and hammer milling (HM) on proso millet flour rheology and baking properties for food application.					
35566206	7	0	theme	artificial	1368:1377	arg1	aging					1379:1383	natural or artificial aging	1357:1383	natural or artificial aging	1357:1383	Pyrograms taken at 300 °C using the Py-GC/MS method can give additional information on the changes in the chemical structure of paper during natural or artificial aging, mainly about the cleavage of β(1→4)-glycosidic bonds during aging.					
35566206	6	1	theme	chemical	1173:1180	arg1	composition					1182:1192	the chemical composition	1169:1192	the chemical composition of the sampled paper	1169:1213	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	3	2	theme	-glycosidic	408:418	arg1	bonds					420:424	cellulose β(1→4)-glycosidic bonds	392:424	cellulose β(1→4)-glycosidic bonds	392:424	One of the degradation products is acetic acid, which can (auto)catalyze the cleavage of cellulose β(1→4)-glycosidic bonds of cellulose polymer chains.					
35566206	7	3	from	changes	1307:1313	arg1	structure					1331:1339	the chemical structure	1318:1339	the chemical structure of paper	1318:1348	Pyrograms taken at 300 °C using the Py-GC/MS method can give additional information on the changes in the chemical structure of paper during natural or artificial aging, mainly about the cleavage of β(1→4)-glycosidic bonds during aging.					
35566206	4	4	theme	Py-GC/MS	479:486	arg1	analysis					488:495	Py-GC/MS analysis	479:495	Py-GC/MS analysis	479:495	However, during 20 s of Py-GC/MS analysis, temperatures of up to 300 °C did not significantly increase or modify the formation of decomposition products of paper components.					
35566206	6	5	theme	aging	1017:1021	arg1	aging					1017:1021	aging	1017:1021	aging	1017:1021	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	6	5	theme	aging	1017:1021	arg1	products					992:999	lignin decomposition products	971:999	lignin decomposition products	971:999	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	6	5	theme	aging	1017:1021	arg1	time					1009:1012	the time	1005:1012	the time of aging	1005:1021	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	6	5	theme	aging	1017:1021	arg1	amount					961:966	the amount	957:966	the amount of lignin decomposition products	957:999	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	3	6	theme	bonds	420:424	arg1	cleavage					380:387	the cleavage	376:387	the cleavage of cellulose β(1→4)-glycosidic bonds of cellulose polymer chains	376:452	One of the degradation products is acetic acid, which can (auto)catalyze the cleavage of cellulose β(1→4)-glycosidic bonds of cellulose polymer chains.					
35566206	1	7	theme	Py-GC/MS	181:188	arg1	method					190:195	the Py-GC/MS method	177:195	the Py-GC/MS method to identify decomposition products	177:230	Samples of papers artificially (2 to 60 days) and naturally (10, 45, and 56 years) aged were studied by the Py-GC/MS method to identify decomposition products.					
35566206	7	8	theme	natural	1357:1363	arg1	aging					1379:1383	natural or artificial aging	1357:1383	natural or artificial aging	1357:1383	Pyrograms taken at 300 °C using the Py-GC/MS method can give additional information on the changes in the chemical structure of paper during natural or artificial aging, mainly about the cleavage of β(1→4)-glycosidic bonds during aging.					
35566206	5	9	dep	increased	695:703	arg1	demonstrated					786:797	demonstrated	786:797	demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products	786:931	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	1	10	dep	60	110:111	arg1	to					107:108	to	107:108	to	107:108	Samples of papers artificially (2 to 60 days) and naturally (10, 45, and 56 years) aged were studied by the Py-GC/MS method to identify decomposition products.					
35566206	5	11	theme	5-hydroxymethylfurfural	850:872	arg1	products					924:931	its consecutive dehydration products	896:931	its consecutive dehydration products	896:931	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	5	11	theme	5-hydroxymethylfurfural	850:872	arg1	generation					813:822	the generation	809:822	the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan	809:890	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	4	12	theme	decomposition	585:597	arg1	products					599:606	decomposition products	585:606	decomposition products of paper components	585:626	However, during 20 s of Py-GC/MS analysis, temperatures of up to 300 °C did not significantly increase or modify the formation of decomposition products of paper components.					
35566206	4	13	theme	paper	611:615	arg1	components					617:626	paper components	611:626	paper components	611:626	However, during 20 s of Py-GC/MS analysis, temperatures of up to 300 °C did not significantly increase or modify the formation of decomposition products of paper components.					
35566206	7	14	theme	paper	1344:1348	arg1	structure					1331:1339	the chemical structure	1318:1339	the chemical structure of paper	1318:1348	Pyrograms taken at 300 °C using the Py-GC/MS method can give additional information on the changes in the chemical structure of paper during natural or artificial aging, mainly about the cleavage of β(1→4)-glycosidic bonds during aging.					
35566206	3	15	theme	polymer	439:445	arg1	chains					447:452	cellulose polymer chains	429:452	cellulose polymer chains	429:452	One of the degradation products is acetic acid, which can (auto)catalyze the cleavage of cellulose β(1→4)-glycosidic bonds of cellulose polymer chains.					
35566206	6	16	theme	products	992:999	arg1	aging					1017:1021	aging	1017:1021	aging	1017:1021	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	6	16	theme	products	992:999	arg1	products					992:999	lignin decomposition products	971:999	lignin decomposition products	971:999	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	6	16	theme	products	992:999	arg1	time					1009:1012	the time	1005:1012	the time of aging	1005:1021	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	6	16	theme	products	992:999	arg1	amount					961:966	the amount	957:966	the amount of lignin decomposition products	957:999	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	4	17	theme	analysis	488:495	arg1	s					474:474	20 s	471:474	20 s of Py-GC/MS analysis	471:495	However, during 20 s of Py-GC/MS analysis, temperatures of up to 300 °C did not significantly increase or modify the formation of decomposition products of paper components.					
35566206	7	18	theme	chemical	1322:1329	arg1	structure					1331:1339	the chemical structure	1318:1339	the chemical structure of paper	1318:1348	Pyrograms taken at 300 °C using the Py-GC/MS method can give additional information on the changes in the chemical structure of paper during natural or artificial aging, mainly about the cleavage of β(1→4)-glycosidic bonds during aging.					
35566206	5	19	theme	several	654:660	arg1	products					686:693	several cellulose decomposition products	654:693	several cellulose decomposition products	654:693	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	3	20	theme	chains	447:452	arg1	cleavage					380:387	the cleavage	376:387	the cleavage of cellulose β(1→4)-glycosidic bonds of cellulose polymer chains	376:452	One of the degradation products is acetic acid, which can (auto)catalyze the cleavage of cellulose β(1→4)-glycosidic bonds of cellulose polymer chains.					
35566206	5	21	theme	artificial	750:759	arg1	aging					761:765	artificial aging	750:765	artificial aging	750:765	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	5	22	theme	aging	761:765	arg1	days					742:745	days	742:745	days of artificial aging and natural aging	742:783	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	3	23	theme	acetic	338:343	arg1	acid					345:348	acetic acid	338:348	acetic acid	338:348	One of the degradation products is acetic acid, which can (auto)catalyze the cleavage of cellulose β(1→4)-glycosidic bonds of cellulose polymer chains.					
35566206	6	24	theme	present	1098:1104	arg1	°C					1073:1074	300 °C and 500 °C. Compounds	1069:1096	°C	1073:1074	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	1	25	theme	papers	84:89	arg1	Samples					73:79	Samples	73:79	Samples of papers artificially (2 to 60 days) and naturally (10, 45, and 56 years) aged	73:159	Samples of papers artificially (2 to 60 days) and naturally (10, 45, and 56 years) aged were studied by the Py-GC/MS method to identify decomposition products.					
35566206	6	26	theme	decomposition	978:990	arg1	products					992:999	lignin decomposition products	971:999	lignin decomposition products	971:999	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	3	27	theme	degradation	314:324	arg1	products					326:333	the degradation products	310:333	the degradation products	310:333	One of the degradation products is acetic acid, which can (auto)catalyze the cleavage of cellulose β(1→4)-glycosidic bonds of cellulose polymer chains.					
35566206	1	28	theme	decomposition	209:221	arg1	products					223:230	decomposition products	209:230	decomposition products	209:230	Samples of papers artificially (2 to 60 days) and naturally (10, 45, and 56 years) aged were studied by the Py-GC/MS method to identify decomposition products.					
35566206	4	29	dep	300	520:522	arg1	to					517:518	to	517:518	to	517:518	However, during 20 s of Py-GC/MS analysis, temperatures of up to 300 °C did not significantly increase or modify the formation of decomposition products of paper components.					
35566206	2	30	theme	reaction	242:249	arg1	scenarios					251:259	Possible reaction scenarios	233:259	Possible reaction scenarios for cellulose degradation	233:285	Possible reaction scenarios for cellulose degradation were developed.					
35566206	4	31	theme	°C	524:525	arg1	temperatures					498:509	temperatures	498:509	temperatures of up to 300 °C	498:525	However, during 20 s of Py-GC/MS analysis, temperatures of up to 300 °C did not significantly increase or modify the formation of decomposition products of paper components.					
35566206	3	32	theme	products	326:333	arg1	One					303:305	One	303:305	One	303:305	One of the degradation products is acetic acid, which can (auto)catalyze the cleavage of cellulose β(1→4)-glycosidic bonds of cellulose polymer chains.					
35566206	3	32	theme	products	326:333	arg1	products					326:333	the degradation products	310:333	the degradation products	310:333	One of the degradation products is acetic acid, which can (auto)catalyze the cleavage of cellulose β(1→4)-glycosidic bonds of cellulose polymer chains.					
35566206	7	33	theme	-glycosidic	1421:1431	arg1	bonds					1433:1437	β(1→4)-glycosidic bonds	1415:1437	β(1→4)-glycosidic bonds	1415:1437	Pyrograms taken at 300 °C using the Py-GC/MS method can give additional information on the changes in the chemical structure of paper during natural or artificial aging, mainly about the cleavage of β(1→4)-glycosidic bonds during aging.					
35566206	5	34	theme	2-furancarboxaldehyde	827:847	arg1	products					924:931	its consecutive dehydration products	896:931	its consecutive dehydration products	896:931	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	5	34	theme	2-furancarboxaldehyde	827:847	arg1	generation					813:822	the generation	809:822	the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan	809:890	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	6	35	theme	°C.	1084:1086	arg1	Compounds					1088:1096	300 °C and 500 °C. Compounds	1069:1096	Compounds	1088:1096	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	2	36	theme	Possible	233:240	arg1	scenarios					251:259	Possible reaction scenarios	233:259	Possible reaction scenarios for cellulose degradation	233:285	Possible reaction scenarios for cellulose degradation were developed.					
35566206	5	37	theme	natural	771:777	arg1	aging					779:783	natural aging	771:783	natural aging	771:783	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	0	38	theme	Cellulose-Based	33:47	arg1	Paper					49:53	Cellulose-Based Paper	33:53	Cellulose-Based Paper	33:53	Accelerated and Natural Aging of Cellulose-Based Paper: Py-GC/MS Method.					
35566206	6	39	attach	present	1098:1104	arg2	°C					1073:1074	300 °C and 500 °C. Compounds	1069:1096	°C	1073:1074	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	6	39	attach	present	1098:1104	arg1	products					1113:1120	the products	1109:1120	the products of decomposition	1109:1137	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	6	40	theme	paper	1209:1213	arg1	composition					1182:1192	the chemical composition	1169:1192	the chemical composition of the sampled paper	1169:1213	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	0	41	theme	Natural	16:22	arg1	Aging					24:28	Natural Aging	16:28	Natural Aging	16:28	Accelerated and Natural Aging of Cellulose-Based Paper: Py-GC/MS Method.					
35566206	5	42	theme	cellulose	662:670	arg1	products					686:693	several cellulose decomposition products	654:693	several cellulose decomposition products	654:693	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	1	43	dep	aged	156:159	arg1	days					113:116	2 to 60 days	105:116	2 to 60 days	105:116	Samples of papers artificially (2 to 60 days) and naturally (10, 45, and 56 years) aged were studied by the Py-GC/MS method to identify decomposition products.					
35566206	1	43	dep	aged	156:159	arg1	years					149:153	10, 45, and 56 years	134:153	10, 45, and 56 years	134:153	Samples of papers artificially (2 to 60 days) and naturally (10, 45, and 56 years) aged were studied by the Py-GC/MS method to identify decomposition products.					
35566206	6	44	theme	decomposition	1125:1137	arg1	products					1113:1120	the products	1109:1120	the products of decomposition	1109:1137	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	7	45	theme	Py-GC/MS	1252:1259	arg1	method					1261:1266	the Py-GC/MS method	1248:1266	the Py-GC/MS method	1248:1266	Pyrograms taken at 300 °C using the Py-GC/MS method can give additional information on the changes in the chemical structure of paper during natural or artificial aging, mainly about the cleavage of β(1→4)-glycosidic bonds during aging.					
35566206	5	46	theme	days	742:745	arg1	number					732:737	the number	728:737	the number of days of artificial aging and natural aging	728:783	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	5	47	theme	decomposition	672:684	arg1	products					686:693	several cellulose decomposition products	654:693	several cellulose decomposition products	654:693	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	6	48	theme	sampled	1201:1207	arg1	paper					1209:1213	the sampled paper	1197:1213	the sampled paper	1197:1213	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	7	49	from	information	1288:1298	arg1	changes					1307:1313	the changes	1303:1313	the changes in the chemical structure of paper during natural or artificial aging	1303:1383	Pyrograms taken at 300 °C using the Py-GC/MS method can give additional information on the changes in the chemical structure of paper during natural or artificial aging, mainly about the cleavage of β(1→4)-glycosidic bonds during aging.					
35566206	5	50	theme	products	686:693	arg1	products					686:693	several cellulose decomposition products	654:693	several cellulose decomposition products	654:693	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	5	50	theme	products	686:693	arg1	amount					644:649	the amount	640:649	the amount of several cellulose decomposition products	640:693	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	1	51	dep	method	190:195	arg1	identify					200:207	identify	200:207	to identify decomposition products	197:230	Samples of papers artificially (2 to 60 days) and naturally (10, 45, and 56 years) aged were studied by the Py-GC/MS method to identify decomposition products.					
35566206	3	52	dep	catalyze	367:374	arg1	auto					362:365	auto	362:365	auto	362:365	One of the degradation products is acetic acid, which can (auto)catalyze the cleavage of cellulose β(1→4)-glycosidic bonds of cellulose polymer chains.					
35566206	4	53	theme	products	599:606	arg1	formation					572:580	the formation	568:580	the formation of decomposition products of paper components	568:626	However, during 20 s of Py-GC/MS analysis, temperatures of up to 300 °C did not significantly increase or modify the formation of decomposition products of paper components.					
35566206	4	54	theme	components	617:626	arg1	products					599:606	decomposition products	585:606	decomposition products of paper components	585:626	However, during 20 s of Py-GC/MS analysis, temperatures of up to 300 °C did not significantly increase or modify the formation of decomposition products of paper components.					
35566206	6	55	from	products	1113:1120	arg1	present					1098:1104	present	1098:1104	present	1098:1104	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	1	56	theme	aged	156:159	arg1	papers					84:89	papers	84:89	papers artificially (2 to 60 days) and naturally (10, 45, and 56 years) aged	84:159	Samples of papers artificially (2 to 60 days) and naturally (10, 45, and 56 years) aged were studied by the Py-GC/MS method to identify decomposition products.					
35566206	6	57	theme	composition	1182:1192	arg1	imprint					1158:1164	the imprint	1154:1164	the imprint of the chemical composition of the sampled paper	1154:1213	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	5	58	theme	consecutive	900:910	arg1	products					924:931	its consecutive dehydration products	896:931	its consecutive dehydration products	896:931	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	2	59	theme	cellulose	265:273	arg1	degradation					275:285	cellulose degradation	265:285	cellulose degradation	265:285	Possible reaction scenarios for cellulose degradation were developed.					
35566206	7	60	theme	additional	1277:1286	arg1	information					1288:1298	additional information	1277:1298	additional information on the changes in the chemical structure of paper during natural or artificial aging	1277:1383	Pyrograms taken at 300 °C using the Py-GC/MS method can give additional information on the changes in the chemical structure of paper during natural or artificial aging, mainly about the cleavage of β(1→4)-glycosidic bonds during aging.					
35566206	5	61	theme	levoglucosan	879:890	arg1	products					924:931	its consecutive dehydration products	896:931	its consecutive dehydration products	896:931	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	5	61	theme	levoglucosan	879:890	arg1	generation					813:822	the generation	809:822	the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan	809:890	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	5	62	theme	dehydration	912:922	arg1	products					924:931	its consecutive dehydration products	896:931	its consecutive dehydration products	896:931	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	6	63	theme	lignin	971:976	arg1	products					992:999	lignin decomposition products	971:999	lignin decomposition products	971:999	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35566206	5	64	theme	aging	779:783	arg1	days					742:745	days	742:745	days of artificial aging and natural aging	742:783	At 300 °C, the amount of several cellulose decomposition products increased regularly depending on the number of days of artificial aging and natural aging, demonstrated mainly by the generation of 2-furancarboxaldehyde, 5-hydroxymethylfurfural, and levoglucosan and its consecutive dehydration products.					
35566206	7	65	theme	bonds	1433:1437	arg1	cleavage					1403:1410	the cleavage	1399:1410	the cleavage of β(1→4)-glycosidic bonds during aging	1399:1450	Pyrograms taken at 300 °C using the Py-GC/MS method can give additional information on the changes in the chemical structure of paper during natural or artificial aging, mainly about the cleavage of β(1→4)-glycosidic bonds during aging.					
35566206	0	66	theme	Py-GC/MS	56:63	arg1	Method					65:70	Py-GC/MS Method	56:70	Accelerated and Natural Aging of Cellulose-Based Paper: Py-GC/MS Method.	0:71	Accelerated and Natural Aging of Cellulose-Based Paper: Py-GC/MS Method.					
35566206	0	67	dep	Accelerated	0:10	arg1	Method					65:70	Py-GC/MS Method	56:70	Accelerated and Natural Aging of Cellulose-Based Paper: Py-GC/MS Method.	0:71	Accelerated and Natural Aging of Cellulose-Based Paper: Py-GC/MS Method.					
35566206	3	68	theme	cellulose	429:437	arg1	chains					447:452	cellulose polymer chains	429:452	cellulose polymer chains	429:452	One of the degradation products is acetic acid, which can (auto)catalyze the cleavage of cellulose β(1→4)-glycosidic bonds of cellulose polymer chains.					
35566206	6	69	from	present	1098:1104	arg1	products					1113:1120	the products	1109:1120	the products of decomposition	1109:1137	No correlation between the amount of lignin decomposition products and the time of aging was found when the pyrolysis was performed at 300 °C and 500 °C. Compounds present in the products of decomposition at 500 °C bear the imprint of the chemical composition of the sampled paper.					
35655126	7	0	dep	lower	1049:1053	arg1	ratio					1070:1074	sucrose/hexose ratio	1055:1074	sucrose/hexose ratio	1055:1074	The L-morph secreted more nectar volume but relatively lower sucrose/hexose ratio than the S-morph.					
35655126	11	1	theme	foraging	1595:1602	arg1	time					1604:1607	the foraging time	1591:1607	the foraging time	1591:1607	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	1	2	theme	heterostylous	270:282	arg1	plants					284:289	heterostylous plants	270:289	heterostylous plants	270:289	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	11	3	theme	more	1511:1514	arg1	grains					1523:1528	more pollen grains	1511:1528	more pollen grains per anther	1511:1539	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	8	4	theme	pollination	1149:1159	arg1	efficiency					1161:1170	its pollination efficiency	1145:1170	its pollination efficiency	1145:1170	Floral visitation rate by hawkmoths was higher but its pollination efficiency was lower in the S-morph than the L-morph.					
35655126	6	5	theme	bumblebee	877:885	arg1	robber					920:925	the nectar robber	909:925	the nectar robber in both morphs	909:940	A bumblebee species functioned as the nectar robber in both morphs and a honeybee as the pollen feeder in the S-morph.					
35655126	6	5	theme	bumblebee	877:885	arg1	species					887:893	A bumblebee species	875:893	A bumblebee species	875:893	A bumblebee species functioned as the nectar robber in both morphs and a honeybee as the pollen feeder in the S-morph.					
35655126	6	5	theme	bumblebee	877:885	arg1	honeybee					948:955	a honeybee	946:955	a honeybee as the pollen feeder in the S-morph	946:991	A bumblebee species functioned as the nectar robber in both morphs and a honeybee as the pollen feeder in the S-morph.					
35655126	1	6	from	traits	260:265	arg1	plants					284:289	heterostylous plants	270:289	heterostylous plants	270:289	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	4	7	theme	nectar	693:698	arg1	volume					700:705	more nectar volume	688:705	more nectar volume	688:705	Both morphs secreted more nectar volume at night than in the day and the sugar composition was rich in sucrose, potentially adaptive to pollination by hawkmoths (Macroglossum spp.)					
35655126	13	8	theme	plant	1920:1924	arg1	sinensis					1929:1936	distylous plant T. sinensis	1910:1936	distylous plant T. sinensis	1910:1936	Ancillary polymorphic floral traits between two morphs are adaptive to hawkmoth and ensure reproductive success in distylous plant T. sinensis.					
35655126	3	9	theme	smaller	604:610	arg1	grains					619:624	more but smaller pollen grains	595:624	more but smaller pollen grains	595:624	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	1	10	theme	reciprocal	201:210	arg1	positions					212:220	reciprocal positions	201:220	reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants	201:289	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	13	11	from	success	1899:1905	arg1	sinensis					1929:1936	distylous plant T. sinensis	1910:1936	distylous plant T. sinensis	1910:1936	Ancillary polymorphic floral traits between two morphs are adaptive to hawkmoth and ensure reproductive success in distylous plant T. sinensis.					
35655126	3	12	theme	more	595:598	arg1	grains					619:624	more but smaller pollen grains	595:624	more but smaller pollen grains	595:624	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	0	13	from	pollination	76:86	arg1	Linaceae					127:134	Linaceae	127:134	Linaceae	127:134	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	0	13	from	pollination	76:86	arg1	sinensis					117:124	distylous plant Tirpitzia sinensis	91:124	distylous plant Tirpitzia sinensis (Linaceae)	91:135	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	2	14	theme	same	404:407	arg1	pollinator					409:418	the same pollinator	400:418	the same pollinator	400:418	However, it remains unclear how differential floral morphs ensure reproductive success between morphs using the same pollinator.					
35655126	9	15	theme	Hand	1215:1218	arg1	treatments					1232:1241	Hand pollination treatments	1215:1241	Hand pollination treatments	1215:1241	Hand pollination treatments indicated self-incompatibility in T. sinensis and seed set of open-pollinated flowers did not differ between morphs.					
35655126	3	16	theme	flower	630:635	arg1	morph					660:664	flower than short-styled (S-) morph	630:664	flower than short-styled (S-) morph	630:664	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	1	17	theme	organs	232:237	arg1	positions					212:220	reciprocal positions	201:220	reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants	201:289	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	7	18	theme	nectar	1020:1025	arg1	volume					1027:1032	nectar volume	1020:1032	nectar volume	1020:1032	The L-morph secreted more nectar volume but relatively lower sucrose/hexose ratio than the S-morph.					
35655126	1	19	from	positions	212:220	arg1	plants					284:289	heterostylous plants	270:289	heterostylous plants	270:289	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	3	20	with	sinensis	487:494	arg1	tubes					514:518	long corolla tubes	501:518	long corolla tubes	501:518	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	3	21	theme	S-	656:657	arg1	morph					660:664	flower than short-styled (S-) morph	630:664	flower than short-styled (S-) morph	630:664	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	12	22	theme	sucrose/hexose	1688:1701	arg1	ratio					1703:1707	a higher sucrose/hexose ratio	1679:1707	a higher sucrose/hexose ratio	1679:1707	The S-morph has a higher sucrose/hexose ratio in its nectar which can be more attractive to hawkmoths and increase the visit rates.					
35655126	3	23	from	Measurements	429:440	arg1	sinensis					487:494	white-flowered Tirpitzia sinensis	462:494	white-flowered Tirpitzia sinensis with long corolla tubes	462:518	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	3	24	theme	short-styled	642:653	arg1	morph					660:664	flower than short-styled (S-) morph	630:664	flower than short-styled (S-) morph	630:664	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	1	25	theme	Floral	149:154	arg1	morphs					156:161	BACKGROUND Floral morphs	138:161	BACKGROUND Floral morphs	138:161	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	10	26	theme	relevant	1443:1450	arg1	traits					1436:1441	traits	1436:1441	traits relevant to pollination	1436:1465	CONCLUSIONS Our findings suggest that the two morphs differ with respect to traits relevant to pollination.					
35655126	4	27	dep	rich	762:765	arg1	composition					746:756	the sugar composition	736:756	the sugar composition	736:756	Both morphs secreted more nectar volume at night than in the day and the sugar composition was rich in sucrose, potentially adaptive to pollination by hawkmoths (Macroglossum spp.)					
35655126	4	27	dep	rich	762:765	arg1	adaptive					791:798	adaptive	791:798	adaptive	791:798	Both morphs secreted more nectar volume at night than in the day and the sugar composition was rich in sucrose, potentially adaptive to pollination by hawkmoths (Macroglossum spp.)					
35655126	4	27	dep	rich	762:765	arg1	rich					762:765	rich	762:765	rich	762:765	Both morphs secreted more nectar volume at night than in the day and the sugar composition was rich in sucrose, potentially adaptive to pollination by hawkmoths (Macroglossum spp.)					
35655126	0	28	theme	distylous	91:99	arg1	Linaceae					127:134	Linaceae	127:134	Linaceae	127:134	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	0	28	theme	distylous	91:99	arg1	sinensis					117:124	distylous plant Tirpitzia sinensis	91:124	distylous plant Tirpitzia sinensis (Linaceae)	91:135	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	3	29	theme	Tirpitzia	477:485	arg1	sinensis					487:494	white-flowered Tirpitzia sinensis	462:494	white-flowered Tirpitzia sinensis with long corolla tubes	462:518	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	0	30	theme	Tirpitzia	107:115	arg1	Linaceae					127:134	Linaceae	127:134	Linaceae	127:134	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	0	30	theme	Tirpitzia	107:115	arg1	sinensis					117:124	distylous plant Tirpitzia sinensis	91:124	distylous plant Tirpitzia sinensis (Linaceae)	91:135	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	9	31	theme	open-pollinated	1305:1319	arg1	flowers					1321:1327	open-pollinated flowers	1305:1327	open-pollinated flowers	1305:1327	Hand pollination treatments indicated self-incompatibility in T. sinensis and seed set of open-pollinated flowers did not differ between morphs.					
35655126	4	32	theme	sugar	740:744	arg1	composition					746:756	the sugar composition	736:756	the sugar composition	736:756	Both morphs secreted more nectar volume at night than in the day and the sugar composition was rich in sucrose, potentially adaptive to pollination by hawkmoths (Macroglossum spp.)					
35655126	4	32	theme	sugar	740:744	arg1	adaptive					791:798	adaptive	791:798	adaptive	791:798	Both morphs secreted more nectar volume at night than in the day and the sugar composition was rich in sucrose, potentially adaptive to pollination by hawkmoths (Macroglossum spp.)					
35655126	4	32	theme	sugar	740:744	arg1	rich					762:765	rich	762:765	rich	762:765	Both morphs secreted more nectar volume at night than in the day and the sugar composition was rich in sucrose, potentially adaptive to pollination by hawkmoths (Macroglossum spp.)					
35655126	0	33	theme	Ancillary	0:8	arg1	traits					29:34	Ancillary polymorphic floral traits	0:34	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).	0:136	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	3	34	dep	distylous	551:559	arg1	producing					585:593	producing	585:593	producing more but smaller pollen grains per flower than short-styled (S-) morph	585:664	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	11	35	dep	has	1507:1509	arg1	increase					1613:1620	increase	1613:1620	increase the pollination efficiency of hawkmoths	1613:1660	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	11	35	dep	has	1507:1509	arg1	prolong					1583:1589	prolong	1583:1589	prolong the foraging time	1583:1607	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	13	36	theme	Ancillary	1795:1803	arg1	traits					1824:1829	Ancillary polymorphic floral traits	1795:1829	Ancillary polymorphic floral traits between two morphs	1795:1848	Ancillary polymorphic floral traits between two morphs are adaptive to hawkmoth and ensure reproductive success in distylous plant T. sinensis.					
35655126	3	37	theme	corolla	506:512	arg1	tubes					514:518	long corolla tubes	501:518	long corolla tubes	501:518	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	0	38	theme	floral	22:27	arg1	traits					29:34	Ancillary polymorphic floral traits	0:34	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).	0:136	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	3	39	theme	floral	445:450	arg1	traits					452:457	floral traits	445:457	floral traits	445:457	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	13	40	theme	floral	1817:1822	arg1	traits					1824:1829	Ancillary polymorphic floral traits	1795:1829	Ancillary polymorphic floral traits between two morphs	1795:1848	Ancillary polymorphic floral traits between two morphs are adaptive to hawkmoth and ensure reproductive success in distylous plant T. sinensis.					
35655126	11	41	contain	has	1507:1509	arg2	volume					1555:1560	a greater volume	1545:1560	a greater volume of nectar	1545:1570	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	11	41	contain	has	1507:1509	arg1	L-morph					1472:1478	The L-morph	1468:1478	The L-morph	1468:1478	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	11	41	contain	has	1507:1509	arg2	grains					1523:1528	more pollen grains	1511:1528	more pollen grains per anther	1511:1539	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	8	42	theme	Floral	1094:1099	arg1	rate					1112:1115	Floral visitation rate	1094:1115	Floral visitation rate by hawkmoths	1094:1128	Floral visitation rate by hawkmoths was higher but its pollination efficiency was lower in the S-morph than the L-morph.					
35655126	1	43	theme	floral	253:258	arg1	traits					260:265	ancillary floral traits	243:265	ancillary floral traits in heterostylous plants	243:289	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	6	44	theme	nectar	913:918	arg1	robber					920:925	the nectar robber	909:925	the nectar robber in both morphs	909:940	A bumblebee species functioned as the nectar robber in both morphs and a honeybee as the pollen feeder in the S-morph.					
35655126	6	44	theme	nectar	913:918	arg1	species					887:893	A bumblebee species	875:893	A bumblebee species	875:893	A bumblebee species functioned as the nectar robber in both morphs and a honeybee as the pollen feeder in the S-morph.					
35655126	4	45	from	night	710:714	arg1	day					728:730	the day	724:730	the day	724:730	Both morphs secreted more nectar volume at night than in the day and the sugar composition was rich in sucrose, potentially adaptive to pollination by hawkmoths (Macroglossum spp.)					
35655126	13	46	theme	distylous	1910:1918	arg1	sinensis					1929:1936	distylous plant T. sinensis	1910:1936	distylous plant T. sinensis	1910:1936	Ancillary polymorphic floral traits between two morphs are adaptive to hawkmoth and ensure reproductive success in distylous plant T. sinensis.					
35655126	7	47	dep	more	1015:1018	arg1	volume					1027:1032	nectar volume	1020:1032	nectar volume	1020:1032	The L-morph secreted more nectar volume but relatively lower sucrose/hexose ratio than the S-morph.					
35655126	11	48	theme	pollen	1516:1521	arg1	grains					1523:1528	more pollen grains	1511:1528	more pollen grains per anther	1511:1539	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	11	49	theme	pollination	1626:1636	arg1	efficiency					1638:1647	the pollination efficiency	1622:1647	the pollination efficiency of hawkmoths	1622:1660	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	7	50	theme	sucrose/hexose	1055:1068	arg1	ratio					1070:1074	sucrose/hexose ratio	1055:1074	sucrose/hexose ratio	1055:1074	The L-morph secreted more nectar volume but relatively lower sucrose/hexose ratio than the S-morph.					
35655126	11	51	theme	greater	1547:1553	arg1	volume					1555:1560	a greater volume	1545:1560	a greater volume of nectar	1545:1570	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	2	52	theme	floral	337:342	arg1	morphs					344:349	differential floral morphs	324:349	differential floral morphs	324:349	However, it remains unclear how differential floral morphs ensure reproductive success between morphs using the same pollinator.					
35655126	10	53	dep	CONCLUSIONS	1360:1370	arg1	suggest					1385:1391	suggest	1385:1391	suggest that the two morphs differ with respect to traits relevant to pollination	1385:1465	CONCLUSIONS Our findings suggest that the two morphs differ with respect to traits relevant to pollination.					
35655126	1	54	from	plants	284:289	arg1	positions					212:220	reciprocal positions	201:220	reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants	201:289	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	13	55	theme	T.	1926:1927	arg1	sinensis					1929:1936	distylous plant T. sinensis	1910:1936	distylous plant T. sinensis	1910:1936	Ancillary polymorphic floral traits between two morphs are adaptive to hawkmoth and ensure reproductive success in distylous plant T. sinensis.					
35655126	2	56	theme	differential	324:335	arg1	morphs					344:349	differential floral morphs	324:349	differential floral morphs	324:349	However, it remains unclear how differential floral morphs ensure reproductive success between morphs using the same pollinator.					
35655126	1	57	theme	sexual	225:230	arg1	organs					232:237	sexual organs	225:237	sexual organs	225:237	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	9	58	theme	pollination	1220:1230	arg1	treatments					1232:1241	Hand pollination treatments	1215:1241	Hand pollination treatments	1215:1241	Hand pollination treatments indicated self-incompatibility in T. sinensis and seed set of open-pollinated flowers did not differ between morphs.					
35655126	3	59	theme	pollen	612:617	arg1	grains					619:624	more but smaller pollen grains	595:624	more but smaller pollen grains	595:624	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	1	60	from	organs	232:237	arg1	plants					284:289	heterostylous plants	270:289	heterostylous plants	270:289	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	13	61	theme	reproductive	1886:1897	arg1	success					1899:1905	reproductive success	1886:1905	reproductive success in distylous plant T. sinensis	1886:1936	Ancillary polymorphic floral traits between two morphs are adaptive to hawkmoth and ensure reproductive success in distylous plant T. sinensis.					
35655126	0	62	theme	hawkmoth	67:74	arg1	pollination					76:86	hawkmoth pollination	67:86	hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae)	67:135	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	3	63	theme	than	637:640	arg1	morph					660:664	flower than short-styled (S-) morph	630:664	flower than short-styled (S-) morph	630:664	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	5	64	from	dusk	869:872	arg1	active					859:864	active	859:864	active	859:864	which were active at dusk.					
35655126	1	65	theme	BACKGROUND	138:147	arg1	morphs					156:161	BACKGROUND Floral morphs	138:161	BACKGROUND Floral morphs	138:161	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	12	66	theme	higher	1681:1686	arg1	ratio					1703:1707	a higher sucrose/hexose ratio	1679:1707	a higher sucrose/hexose ratio	1679:1707	The S-morph has a higher sucrose/hexose ratio in its nectar which can be more attractive to hawkmoths and increase the visit rates.					
35655126	1	67	from	differentiations	181:196	arg1	positions					212:220	reciprocal positions	201:220	reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants	201:289	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	11	68	theme	exserted	1490:1497	arg1	stigma					1499:1504	its exserted stigma	1486:1504	its exserted stigma	1486:1504	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	0	69	theme	plant	101:105	arg1	Linaceae					127:134	Linaceae	127:134	Linaceae	127:134	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	0	69	theme	plant	101:105	arg1	sinensis					117:124	distylous plant Tirpitzia sinensis	91:124	distylous plant Tirpitzia sinensis (Linaceae)	91:135	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	9	70	theme	seed	1293:1296	arg1	set					1298:1300	seed set	1293:1300	seed set of open-pollinated flowers	1293:1327	Hand pollination treatments indicated self-incompatibility in T. sinensis and seed set of open-pollinated flowers did not differ between morphs.					
35655126	9	70	theme	seed	1293:1296	arg1	flowers					1321:1327	open-pollinated flowers	1305:1327	open-pollinated flowers	1305:1327	Hand pollination treatments indicated self-incompatibility in T. sinensis and seed set of open-pollinated flowers did not differ between morphs.					
35655126	4	71	from	sucrose	770:776	arg1	composition					746:756	the sugar composition	736:756	the sugar composition	736:756	Both morphs secreted more nectar volume at night than in the day and the sugar composition was rich in sucrose, potentially adaptive to pollination by hawkmoths (Macroglossum spp.)					
35655126	4	71	from	sucrose	770:776	arg1	adaptive					791:798	adaptive	791:798	adaptive	791:798	Both morphs secreted more nectar volume at night than in the day and the sugar composition was rich in sucrose, potentially adaptive to pollination by hawkmoths (Macroglossum spp.)					
35655126	4	71	from	sucrose	770:776	arg1	rich					762:765	rich	762:765	rich	762:765	Both morphs secreted more nectar volume at night than in the day and the sugar composition was rich in sucrose, potentially adaptive to pollination by hawkmoths (Macroglossum spp.)					
35655126	6	72	theme	pollen	964:969	arg1	feeder					971:976	the pollen feeder	960:976	the pollen feeder in the S-morph	960:991	A bumblebee species functioned as the nectar robber in both morphs and a honeybee as the pollen feeder in the S-morph.					
35655126	11	73	with	L-morph	1472:1478	arg1	stigma					1499:1504	its exserted stigma	1486:1504	its exserted stigma	1486:1504	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	4	74	from	rich	762:765	arg1	sucrose					770:776	sucrose	770:776	sucrose	770:776	Both morphs secreted more nectar volume at night than in the day and the sugar composition was rich in sucrose, potentially adaptive to pollination by hawkmoths (Macroglossum spp.)					
35655126	0	75	theme	polymorphic	10:20	arg1	traits					29:34	Ancillary polymorphic floral traits	0:34	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).	0:136	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	9	76	theme	flowers	1321:1327	arg1	set					1298:1300	seed set	1293:1300	seed set of open-pollinated flowers	1293:1327	Hand pollination treatments indicated self-incompatibility in T. sinensis and seed set of open-pollinated flowers did not differ between morphs.					
35655126	9	76	theme	flowers	1321:1327	arg1	flowers					1321:1327	open-pollinated flowers	1305:1327	open-pollinated flowers	1305:1327	Hand pollination treatments indicated self-incompatibility in T. sinensis and seed set of open-pollinated flowers did not differ between morphs.					
35655126	3	77	theme	RESULTS	421:427	arg1	Measurements					429:440	RESULTS Measurements	421:440	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes	421:518	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	6	78	from	feeder	971:976	arg1	S-morph					985:991	the S-morph	981:991	the S-morph	981:991	A bumblebee species functioned as the nectar robber in both morphs and a honeybee as the pollen feeder in the S-morph.					
35655126	3	79	theme	white-flowered	462:475	arg1	sinensis					487:494	white-flowered Tirpitzia sinensis	462:494	white-flowered Tirpitzia sinensis with long corolla tubes	462:518	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	13	80	theme	polymorphic	1805:1815	arg1	traits					1824:1829	Ancillary polymorphic floral traits	1795:1829	Ancillary polymorphic floral traits between two morphs	1795:1848	Ancillary polymorphic floral traits between two morphs are adaptive to hawkmoth and ensure reproductive success in distylous plant T. sinensis.					
35655126	3	81	theme	traits	452:457	arg1	Measurements					429:440	RESULTS Measurements	421:440	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes	421:518	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	6	82	from	honeybee	948:955	arg1	morphs					935:940	both morphs	930:940	both morphs	930:940	A bumblebee species functioned as the nectar robber in both morphs and a honeybee as the pollen feeder in the S-morph.					
35655126	11	83	theme	hawkmoths	1652:1660	arg1	efficiency					1638:1647	the pollination efficiency	1622:1647	the pollination efficiency of hawkmoths	1622:1660	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	11	84	theme	nectar	1565:1570	arg1	grains					1523:1528	more pollen grains	1511:1528	more pollen grains per anther	1511:1539	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	11	84	theme	nectar	1565:1570	arg1	volume					1555:1560	a greater volume	1545:1560	a greater volume of nectar	1545:1570	The L-morph, with its exserted stigma, has more pollen grains per anther and a greater volume of nectar, which may prolong the foraging time and increase the pollination efficiency of hawkmoths.					
35655126	3	85	theme	long	501:504	arg1	tubes					514:518	long corolla tubes	501:518	long corolla tubes	501:518	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	0	86	theme	adaptive	55:62	arg1	morphs					48:53	two morphs	44:53	two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae)	44:135	Ancillary polymorphic floral traits between two morphs adaptive to hawkmoth pollination in distylous plant Tirpitzia sinensis (Linaceae).					
35655126	6	87	from	robber	920:925	arg1	morphs					935:940	both morphs	930:940	both morphs	930:940	A bumblebee species functioned as the nectar robber in both morphs and a honeybee as the pollen feeder in the S-morph.					
35655126	2	88	theme	reproductive	358:369	arg1	success					371:377	reproductive success	358:377	reproductive success between morphs using the same pollinator	358:418	However, it remains unclear how differential floral morphs ensure reproductive success between morphs using the same pollinator.					
35655126	8	89	theme	visitation	1101:1110	arg1	rate					1112:1115	Floral visitation rate	1094:1115	Floral visitation rate by hawkmoths	1094:1128	Floral visitation rate by hawkmoths was higher but its pollination efficiency was lower in the S-morph than the L-morph.					
35655126	12	90	theme	visit	1782:1786	arg1	rates					1788:1792	the visit rates	1778:1792	the visit rates	1778:1792	The S-morph has a higher sucrose/hexose ratio in its nectar which can be more attractive to hawkmoths and increase the visit rates.					
35655126	12	91	contain	has	1675:1677	arg2	ratio					1703:1707	a higher sucrose/hexose ratio	1679:1707	a higher sucrose/hexose ratio	1679:1707	The S-morph has a higher sucrose/hexose ratio in its nectar which can be more attractive to hawkmoths and increase the visit rates.					
35655126	12	91	contain	has	1675:1677	arg1	S-morph					1667:1673	The S-morph	1663:1673	The S-morph	1663:1673	The S-morph has a higher sucrose/hexose ratio in its nectar which can be more attractive to hawkmoths and increase the visit rates.					
35655126	1	92	theme	ancillary	243:251	arg1	traits					260:265	ancillary floral traits	243:265	ancillary floral traits in heterostylous plants	243:289	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
35655126	3	93	theme	long-styled	562:572	arg1	morph					579:583	long-styled (L-) morph	562:583	long-styled (L-) morph	562:583	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	3	93	theme	long-styled	562:572	arg1	L-					575:576	L-	575:576	L-	575:576	RESULTS Measurements of floral traits in white-flowered Tirpitzia sinensis with long corolla tubes indicated that it is typically distylous, long-styled (L-) morph producing more but smaller pollen grains per flower than short-styled (S-) morph.					
35655126	1	94	theme	traits	260:265	arg1	positions					212:220	reciprocal positions	201:220	reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants	201:289	BACKGROUND Floral morphs are characterized differentiations in reciprocal positions of sexual organs and ancillary floral traits in heterostylous plants.					
36435473	5	0	theme	maximal	749:755	arg1	IC50					783:786	IC50	783:786	IC50	783:786	In particular, ATL, with a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1, exhibited superior antioxidant activity than butylated hydroxytoluene (38.3 μg·mL-1) and KL (50.0 μg·mL-1).					
36435473	5	0	theme	maximal	749:755	arg1	concentration					768:780	a half maximal inhibitory concentration	742:780	a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1	742:803	In particular, ATL, with a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1, exhibited superior antioxidant activity than butylated hydroxytoluene (38.3 μg·mL-1) and KL (50.0 μg·mL-1).					
36435473	8	1	link	lignin-derived	1265:1278	arg1	properties					1296:1305	lignin-derived multifunctional properties	1265:1305	lignin-derived multifunctional properties	1265:1305	Therefore, ATL designed with lignin-derived multifunctional properties has potential applications as an antioxidant and UV-shielding bio-additive and shows significant prospects in food packaging and biomedical applications.					
36435473	4	2	theme	-shielding	694:703	arg1	properties					705:714	antioxidant and ultraviolet (UV)-shielding properties	662:714	antioxidant and ultraviolet (UV)-shielding properties	662:714	ATL with adequate acetoacetyl groups exhibited enhanced molecular weight and antioxidant and ultraviolet (UV)-shielding properties.					
36435473	5	3	theme	superior	816:823	arg1	activity					837:844	superior antioxidant activity	816:844	superior antioxidant activity	816:844	In particular, ATL, with a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1, exhibited superior antioxidant activity than butylated hydroxytoluene (38.3 μg·mL-1) and KL (50.0 μg·mL-1).					
36435473	1	4	theme	sustainable	173:183	arg1	composites					236:245	eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	159:245	eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	159:245	The development of eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites is becoming increasingly important.					
36435473	0	5	theme	lignin	88:93	arg1	biofilms					54:61	chitosan-based biofilms	39:61	chitosan-based biofilms of novel acetoacetylated lignin	39:93	Eco-friendly and facile preparation of chitosan-based biofilms of novel acetoacetylated lignin for antioxidant and UV-shielding properties.					
36435473	2	6	theme	one-step	368:375	arg1	reaction					387:394	an eco-friendly, facile one-step synthesis reaction	344:394	an eco-friendly, facile one-step synthesis reaction	344:394	In this study, acetoacetylated lignin (ATL) was obtained via an eco-friendly, facile one-step synthesis reaction, and chitosan (CS)-containing ATL films (CSL) were prepared.					
36435473	7	7	theme	CSL	1159:1161	arg1	properties					1137:1146	the UV-A and UV-B shielding properties	1109:1146	properties	1137:1146	Notably, the UV-A and UV-B shielding properties of the 2 % CSL were 130 % and 78 % higher than those of the pure CS film, respectively.					
36435473	7	7	theme	CSL	1159:1161	arg1	UV-A					1113:1116	the UV-A and UV-B shielding properties	1109:1146	UV-A	1113:1116	Notably, the UV-A and UV-B shielding properties of the 2 % CSL were 130 % and 78 % higher than those of the pure CS film, respectively.					
36435473	7	7	theme	CSL	1159:1161	arg1	%					1172:1172	130 %	1168:1172	130 %	1168:1172	Notably, the UV-A and UV-B shielding properties of the 2 % CSL were 130 % and 78 % higher than those of the pure CS film, respectively.					
36435473	8	8	theme	lignin-derived	1265:1278	arg1	properties					1296:1305	lignin-derived multifunctional properties	1265:1305	lignin-derived multifunctional properties	1265:1305	Therefore, ATL designed with lignin-derived multifunctional properties has potential applications as an antioxidant and UV-shielding bio-additive and shows significant prospects in food packaging and biomedical applications.					
36435473	0	9	theme	antioxidant	99:109	arg1	properties					128:137	antioxidant and UV-shielding properties	99:137	antioxidant and UV-shielding properties	99:137	Eco-friendly and facile preparation of chitosan-based biofilms of novel acetoacetylated lignin for antioxidant and UV-shielding properties.					
36435473	6	10	dep	activity	998:1005	arg1	the					982:984	the	982:984	the	982:984	When ATL was incorporated into the CS solution to prepare biofilms, the antioxidant activity, UV-shielding property, water resistance, and thermal stability of the CSL greatly improved.					
36435473	7	11	theme	higher	1183:1188	arg1	%					1181:1181	78 %	1178:1181	78 % higher than those of the pure CS film	1178:1219	Notably, the UV-A and UV-B shielding properties of the 2 % CSL were 130 % and 78 % higher than those of the pure CS film, respectively.					
36435473	2	12	theme	synthesis	377:385	arg1	reaction					387:394	an eco-friendly, facile one-step synthesis reaction	344:394	an eco-friendly, facile one-step synthesis reaction	344:394	In this study, acetoacetylated lignin (ATL) was obtained via an eco-friendly, facile one-step synthesis reaction, and chitosan (CS)-containing ATL films (CSL) were prepared.					
36435473	8	13	contain	has	1307:1309	arg2	applications					1321:1332	potential applications	1311:1332	potential applications	1311:1332	Therefore, ATL designed with lignin-derived multifunctional properties has potential applications as an antioxidant and UV-shielding bio-additive and shows significant prospects in food packaging and biomedical applications.					
36435473	8	13	contain	has	1307:1309	arg1	ATL					1247:1249	ATL	1247:1249	ATL designed with lignin-derived multifunctional properties	1247:1305	Therefore, ATL designed with lignin-derived multifunctional properties has potential applications as an antioxidant and UV-shielding bio-additive and shows significant prospects in food packaging and biomedical applications.					
36435473	8	13	contain	has	1307:1309	arg2	antioxidant					1340:1350	antioxidant	1340:1350	antioxidant	1340:1350	Therefore, ATL designed with lignin-derived multifunctional properties has potential applications as an antioxidant and UV-shielding bio-additive and shows significant prospects in food packaging and biomedical applications.					
36435473	5	14	theme	butylated	851:859	arg1	hydroxytoluene					861:874	butylated hydroxytoluene	851:874	butylated hydroxytoluene (38.3 μg·mL-1)	851:889	In particular, ATL, with a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1, exhibited superior antioxidant activity than butylated hydroxytoluene (38.3 μg·mL-1) and KL (50.0 μg·mL-1).					
36435473	5	14	theme	butylated	851:859	arg1	38.3 μg·mL-1					877:888	38.3 μg·mL-1	877:888	38.3 μg·mL-1	877:888	In particular, ATL, with a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1, exhibited superior antioxidant activity than butylated hydroxytoluene (38.3 μg·mL-1) and KL (50.0 μg·mL-1).					
36435473	7	15	theme	2 	1155:1156	arg1	%					1157:1157	%	1157:1157	%	1157:1157	Notably, the UV-A and UV-B shielding properties of the 2 % CSL were 130 % and 78 % higher than those of the pure CS film, respectively.					
36435473	7	16	theme	pure	1208:1211	arg1	film					1216:1219	the pure CS film	1204:1219	the pure CS film	1204:1219	Notably, the UV-A and UV-B shielding properties of the 2 % CSL were 130 % and 78 % higher than those of the pure CS film, respectively.					
36435473	5	17	theme	antioxidant	825:835	arg1	activity					837:844	superior antioxidant activity	816:844	superior antioxidant activity	816:844	In particular, ATL, with a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1, exhibited superior antioxidant activity than butylated hydroxytoluene (38.3 μg·mL-1) and KL (50.0 μg·mL-1).					
36435473	2	18	theme	ATL	426:428	arg1	CSL					437:439	CSL	437:439	CSL	437:439	In this study, acetoacetylated lignin (ATL) was obtained via an eco-friendly, facile one-step synthesis reaction, and chitosan (CS)-containing ATL films (CSL) were prepared.					
36435473	2	18	theme	ATL	426:428	arg1	films					430:434	chitosan (CS)-containing ATL films	401:434	chitosan (CS)-containing ATL films (CSL)	401:440	In this study, acetoacetylated lignin (ATL) was obtained via an eco-friendly, facile one-step synthesis reaction, and chitosan (CS)-containing ATL films (CSL) were prepared.					
36435473	6	19	theme	UV-shielding	1008:1019	arg1	property					1021:1028	UV-shielding property	1008:1028	UV-shielding property	1008:1028	When ATL was incorporated into the CS solution to prepare biofilms, the antioxidant activity, UV-shielding property, water resistance, and thermal stability of the CSL greatly improved.					
36435473	1	20	theme	biodegradable	186:198	arg1	composites					236:245	eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	159:245	eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	159:245	The development of eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites is becoming increasingly important.					
36435473	0	21	theme	UV-shielding	115:126	arg1	properties					128:137	antioxidant and UV-shielding properties	99:137	antioxidant and UV-shielding properties	99:137	Eco-friendly and facile preparation of chitosan-based biofilms of novel acetoacetylated lignin for antioxidant and UV-shielding properties.					
36435473	2	22	theme	acetoacetylated	298:312	arg1	ATL					322:324	ATL	322:324	ATL	322:324	In this study, acetoacetylated lignin (ATL) was obtained via an eco-friendly, facile one-step synthesis reaction, and chitosan (CS)-containing ATL films (CSL) were prepared.					
36435473	2	22	theme	acetoacetylated	298:312	arg1	lignin					314:319	acetoacetylated lignin	298:319	acetoacetylated lignin (ATL)	298:325	In this study, acetoacetylated lignin (ATL) was obtained via an eco-friendly, facile one-step synthesis reaction, and chitosan (CS)-containing ATL films (CSL) were prepared.					
36435473	6	23	theme	thermal	1053:1059	arg1	stability					1061:1069	thermal stability	1053:1069	thermal stability	1053:1069	When ATL was incorporated into the CS solution to prepare biofilms, the antioxidant activity, UV-shielding property, water resistance, and thermal stability of the CSL greatly improved.					
36435473	5	24	theme	inhibitory	757:766	arg1	IC50					783:786	IC50	783:786	IC50	783:786	In particular, ATL, with a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1, exhibited superior antioxidant activity than butylated hydroxytoluene (38.3 μg·mL-1) and KL (50.0 μg·mL-1).					
36435473	5	24	theme	inhibitory	757:766	arg1	concentration					768:780	a half maximal inhibitory concentration	742:780	a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1	742:803	In particular, ATL, with a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1, exhibited superior antioxidant activity than butylated hydroxytoluene (38.3 μg·mL-1) and KL (50.0 μg·mL-1).					
36435473	2	25	dep	eco-friendly	347:358	arg1	facile					361:366	facile	361:366	facile	361:366	In this study, acetoacetylated lignin (ATL) was obtained via an eco-friendly, facile one-step synthesis reaction, and chitosan (CS)-containing ATL films (CSL) were prepared.					
36435473	5	26	theme	23.8 μg·mL-1	792:803	arg1	IC50					783:786	IC50	783:786	IC50	783:786	In particular, ATL, with a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1, exhibited superior antioxidant activity than butylated hydroxytoluene (38.3 μg·mL-1) and KL (50.0 μg·mL-1).					
36435473	5	26	theme	23.8 μg·mL-1	792:803	arg1	concentration					768:780	a half maximal inhibitory concentration	742:780	a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1	742:803	In particular, ATL, with a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1, exhibited superior antioxidant activity than butylated hydroxytoluene (38.3 μg·mL-1) and KL (50.0 μg·mL-1).					
36435473	1	27	theme	biocompatible	205:217	arg1	composites					236:245	eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	159:245	eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	159:245	The development of eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites is becoming increasingly important.					
36435473	7	28	theme	shielding	1127:1135	arg1	properties					1137:1146	the UV-A and UV-B shielding properties	1109:1146	properties	1137:1146	Notably, the UV-A and UV-B shielding properties of the 2 % CSL were 130 % and 78 % higher than those of the pure CS film, respectively.					
36435473	1	29	theme	green	219:223	arg1	composites					236:245	eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	159:245	eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	159:245	The development of eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites is becoming increasingly important.					
36435473	0	30	theme	facile	17:22	arg1	preparation					24:34	facile preparation	17:34	facile preparation	17:34	Eco-friendly and facile preparation of chitosan-based biofilms of novel acetoacetylated lignin for antioxidant and UV-shielding properties.					
36435473	6	31	theme	CS	949:950	arg1	solution					952:959	the CS solution	945:959	the CS solution	945:959	When ATL was incorporated into the CS solution to prepare biofilms, the antioxidant activity, UV-shielding property, water resistance, and thermal stability of the CSL greatly improved.					
36435473	3	32	theme	kraft	566:570	arg1	KL					580:581	KL	580:581	KL	580:581	The chemical structural analysis of ATL confirmed that the acetoacetyl groups were successfully grafted onto kraft lignin (KL).					
36435473	3	32	theme	kraft	566:570	arg1	lignin					572:577	kraft lignin	566:577	kraft lignin (KL)	566:582	The chemical structural analysis of ATL confirmed that the acetoacetyl groups were successfully grafted onto kraft lignin (KL).					
36435473	1	33	theme	biopolymer	225:234	arg1	composites					236:245	eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	159:245	eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	159:245	The development of eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites is becoming increasingly important.					
36435473	0	34	theme	chitosan-based	39:52	arg1	biofilms					54:61	chitosan-based biofilms	39:61	chitosan-based biofilms of novel acetoacetylated lignin	39:93	Eco-friendly and facile preparation of chitosan-based biofilms of novel acetoacetylated lignin for antioxidant and UV-shielding properties.					
36435473	7	35	theme	CS	1213:1214	arg1	film					1216:1219	the pure CS film	1204:1219	the pure CS film	1204:1219	Notably, the UV-A and UV-B shielding properties of the 2 % CSL were 130 % and 78 % higher than those of the pure CS film, respectively.					
36435473	1	36	theme	composites	236:245	arg1	development					144:154	The development	140:154	The development of eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	140:245	The development of eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites is becoming increasingly important.					
36435473	2	37	theme	-containing	414:424	arg1	CSL					437:439	CSL	437:439	CSL	437:439	In this study, acetoacetylated lignin (ATL) was obtained via an eco-friendly, facile one-step synthesis reaction, and chitosan (CS)-containing ATL films (CSL) were prepared.					
36435473	2	37	theme	-containing	414:424	arg1	films					430:434	chitosan (CS)-containing ATL films	401:434	chitosan (CS)-containing ATL films (CSL)	401:440	In this study, acetoacetylated lignin (ATL) was obtained via an eco-friendly, facile one-step synthesis reaction, and chitosan (CS)-containing ATL films (CSL) were prepared.					
36435473	4	38	theme	antioxidant	662:672	arg1	properties					705:714	antioxidant and ultraviolet (UV)-shielding properties	662:714	antioxidant and ultraviolet (UV)-shielding properties	662:714	ATL with adequate acetoacetyl groups exhibited enhanced molecular weight and antioxidant and ultraviolet (UV)-shielding properties.					
36435473	7	39	theme	UV-B	1122:1125	arg1	properties					1137:1146	the UV-A and UV-B shielding properties	1109:1146	properties	1137:1146	Notably, the UV-A and UV-B shielding properties of the 2 % CSL were 130 % and 78 % higher than those of the pure CS film, respectively.					
36435473	6	40	theme	water	1031:1035	arg1	resistance					1037:1046	water resistance	1031:1046	water resistance	1031:1046	When ATL was incorporated into the CS solution to prepare biofilms, the antioxidant activity, UV-shielding property, water resistance, and thermal stability of the CSL greatly improved.					
36435473	4	41	theme	molecular	641:649	arg1	weight					651:656	enhanced molecular weight	632:656	enhanced molecular weight	632:656	ATL with adequate acetoacetyl groups exhibited enhanced molecular weight and antioxidant and ultraviolet (UV)-shielding properties.					
36435473	8	42	theme	potential	1311:1319	arg1	applications					1321:1332	potential applications	1311:1332	potential applications	1311:1332	Therefore, ATL designed with lignin-derived multifunctional properties has potential applications as an antioxidant and UV-shielding bio-additive and shows significant prospects in food packaging and biomedical applications.					
36435473	8	42	theme	potential	1311:1319	arg1	antioxidant					1340:1350	antioxidant	1340:1350	antioxidant	1340:1350	Therefore, ATL designed with lignin-derived multifunctional properties has potential applications as an antioxidant and UV-shielding bio-additive and shows significant prospects in food packaging and biomedical applications.					
36435473	8	43	theme	food	1417:1420	arg1	packaging					1422:1430	food packaging	1417:1430	food packaging	1417:1430	Therefore, ATL designed with lignin-derived multifunctional properties has potential applications as an antioxidant and UV-shielding bio-additive and shows significant prospects in food packaging and biomedical applications.					
36435473	3	44	theme	chemical	461:468	arg1	analysis					481:488	The chemical structural analysis	457:488	The chemical structural analysis of ATL	457:495	The chemical structural analysis of ATL confirmed that the acetoacetyl groups were successfully grafted onto kraft lignin (KL).					
36435473	0	45	theme	biofilms	54:61	arg1	Eco-friendly					0:11	Eco-friendly	0:11	Eco-friendly	0:11	Eco-friendly and facile preparation of chitosan-based biofilms of novel acetoacetylated lignin for antioxidant and UV-shielding properties.					
36435473	0	45	theme	biofilms	54:61	arg1	preparation					24:34	facile preparation	17:34	facile preparation	17:34	Eco-friendly and facile preparation of chitosan-based biofilms of novel acetoacetylated lignin for antioxidant and UV-shielding properties.					
36435473	7	46	theme	%	1157:1157	arg1	CSL					1159:1161	the 2 % CSL	1151:1161	the 2 % CSL	1151:1161	Notably, the UV-A and UV-B shielding properties of the 2 % CSL were 130 % and 78 % higher than those of the pure CS film, respectively.					
36435473	8	47	theme	biomedical	1436:1445	arg1	applications					1447:1458	biomedical applications	1436:1458	biomedical applications	1436:1458	Therefore, ATL designed with lignin-derived multifunctional properties has potential applications as an antioxidant and UV-shielding bio-additive and shows significant prospects in food packaging and biomedical applications.					
36435473	5	48	with	ATL	732:734	arg1	IC50					783:786	IC50	783:786	IC50	783:786	In particular, ATL, with a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1, exhibited superior antioxidant activity than butylated hydroxytoluene (38.3 μg·mL-1) and KL (50.0 μg·mL-1).					
36435473	5	48	with	ATL	732:734	arg1	concentration					768:780	a half maximal inhibitory concentration	742:780	a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1	742:803	In particular, ATL, with a half maximal inhibitory concentration (IC50) of 23.8 μg·mL-1, exhibited superior antioxidant activity than butylated hydroxytoluene (38.3 μg·mL-1) and KL (50.0 μg·mL-1).					
36435473	3	49	theme	ATL	493:495	arg1	analysis					481:488	The chemical structural analysis	457:488	The chemical structural analysis of ATL	457:495	The chemical structural analysis of ATL confirmed that the acetoacetyl groups were successfully grafted onto kraft lignin (KL).					
36435473	4	50	with	ATL	585:587	arg1	groups					615:620	adequate acetoacetyl groups	594:620	adequate acetoacetyl groups	594:620	ATL with adequate acetoacetyl groups exhibited enhanced molecular weight and antioxidant and ultraviolet (UV)-shielding properties.					
36435473	2	51	theme	eco-friendly	347:358	arg1	reaction					387:394	an eco-friendly, facile one-step synthesis reaction	344:394	an eco-friendly, facile one-step synthesis reaction	344:394	In this study, acetoacetylated lignin (ATL) was obtained via an eco-friendly, facile one-step synthesis reaction, and chitosan (CS)-containing ATL films (CSL) were prepared.					
36435473	4	52	theme	adequate	594:601	arg1	groups					615:620	adequate acetoacetyl groups	594:620	adequate acetoacetyl groups	594:620	ATL with adequate acetoacetyl groups exhibited enhanced molecular weight and antioxidant and ultraviolet (UV)-shielding properties.					
36435473	8	53	theme	multifunctional	1280:1294	arg1	properties					1296:1305	lignin-derived multifunctional properties	1265:1305	lignin-derived multifunctional properties	1265:1305	Therefore, ATL designed with lignin-derived multifunctional properties has potential applications as an antioxidant and UV-shielding bio-additive and shows significant prospects in food packaging and biomedical applications.					
36435473	6	54	theme	CSL	1078:1080	arg1	activity					998:1005	antioxidant activity	986:1005	antioxidant activity	986:1005	When ATL was incorporated into the CS solution to prepare biofilms, the antioxidant activity, UV-shielding property, water resistance, and thermal stability of the CSL greatly improved.					
36435473	6	54	theme	CSL	1078:1080	arg1	property					1021:1028	UV-shielding property	1008:1028	UV-shielding property	1008:1028	When ATL was incorporated into the CS solution to prepare biofilms, the antioxidant activity, UV-shielding property, water resistance, and thermal stability of the CSL greatly improved.					
36435473	6	54	theme	CSL	1078:1080	arg1	resistance					1037:1046	water resistance	1031:1046	water resistance	1031:1046	When ATL was incorporated into the CS solution to prepare biofilms, the antioxidant activity, UV-shielding property, water resistance, and thermal stability of the CSL greatly improved.					
36435473	6	54	theme	CSL	1078:1080	arg1	stability					1061:1069	thermal stability	1053:1069	thermal stability	1053:1069	When ATL was incorporated into the CS solution to prepare biofilms, the antioxidant activity, UV-shielding property, water resistance, and thermal stability of the CSL greatly improved.					
36435473	3	55	theme	structural	470:479	arg1	analysis					481:488	The chemical structural analysis	457:488	The chemical structural analysis of ATL	457:495	The chemical structural analysis of ATL confirmed that the acetoacetyl groups were successfully grafted onto kraft lignin (KL).					
36435473	0	56	theme	acetoacetylated	72:86	arg1	lignin					88:93	novel acetoacetylated lignin	66:93	novel acetoacetylated lignin	66:93	Eco-friendly and facile preparation of chitosan-based biofilms of novel acetoacetylated lignin for antioxidant and UV-shielding properties.					
36435473	8	57	theme	significant	1392:1402	arg1	prospects					1404:1412	significant prospects	1392:1412	significant prospects	1392:1412	Therefore, ATL designed with lignin-derived multifunctional properties has potential applications as an antioxidant and UV-shielding bio-additive and shows significant prospects in food packaging and biomedical applications.					
36435473	4	58	theme	enhanced	632:639	arg1	weight					651:656	enhanced molecular weight	632:656	enhanced molecular weight	632:656	ATL with adequate acetoacetyl groups exhibited enhanced molecular weight and antioxidant and ultraviolet (UV)-shielding properties.					
36435473	1	59	theme	eco-friendly	159:170	arg1	composites					236:245	eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	159:245	eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites	159:245	The development of eco-friendly, sustainable, biodegradable, and biocompatible green biopolymer composites is becoming increasingly important.					
36435473	0	60	theme	novel	66:70	arg1	lignin					88:93	novel acetoacetylated lignin	66:93	novel acetoacetylated lignin	66:93	Eco-friendly and facile preparation of chitosan-based biofilms of novel acetoacetylated lignin for antioxidant and UV-shielding properties.					
36435473	6	61	theme	antioxidant	986:996	arg1	activity					998:1005	antioxidant activity	986:1005	antioxidant activity	986:1005	When ATL was incorporated into the CS solution to prepare biofilms, the antioxidant activity, UV-shielding property, water resistance, and thermal stability of the CSL greatly improved.					
36435473	3	62	theme	acetoacetyl	516:526	arg1	groups					528:533	the acetoacetyl groups	512:533	the acetoacetyl groups	512:533	The chemical structural analysis of ATL confirmed that the acetoacetyl groups were successfully grafted onto kraft lignin (KL).					
36435473	4	63	theme	acetoacetyl	603:613	arg1	groups					615:620	adequate acetoacetyl groups	594:620	adequate acetoacetyl groups	594:620	ATL with adequate acetoacetyl groups exhibited enhanced molecular weight and antioxidant and ultraviolet (UV)-shielding properties.					
36280324	1	0	theme	commensal	137:145	arg1	microorganisms					147:160	symbiotic or commensal microorganisms	124:160	symbiotic or commensal microorganisms	124:160	Since birth, the human body gets colonized by various communities of symbiotic or commensal microorganisms and they persist till the death of an individual.					
36280324	7	1	theme	various	1114:1120	arg1	disease					1226:1232	inflammatory bowel disease	1207:1232	inflammatory bowel disease (IBD)	1207:1238	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	1	theme	various	1114:1120	arg1	periodontitis					1249:1261	periodontitis	1249:1261	periodontitis	1249:1261	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	1	theme	various	1114:1120	arg1	obesity					1268:1274	obesity	1268:1274	obesity	1268:1274	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	1	theme	various	1114:1120	arg1	infections					1122:1131	various infections	1114:1131	various infections	1114:1131	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	1	theme	various	1114:1120	arg1	cancer					1241:1246	cancer	1241:1246	cancer	1241:1246	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	1	theme	various	1114:1120	arg1	infection					1190:1198	Clostridioides difficile infection	1165:1198	Clostridioides difficile infection (CFI)	1165:1204	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	1	2	theme	microorganisms	147:160	arg1	communities					109:119	various communities	101:119	various communities of symbiotic or commensal microorganisms	101:160	Since birth, the human body gets colonized by various communities of symbiotic or commensal microorganisms and they persist till the death of an individual.					
36280324	2	3	theme	microorganisms	264:277	arg1	genomes					253:259	the genomes	249:259	the genomes of microorganisms such as viruses, archaea, eukaryotes, protozoa, and, most remarkably, bacteria	249:356	The human microbiome is comprised of the genomes of microorganisms such as viruses, archaea, eukaryotes, protozoa, and, most remarkably, bacteria.					
36280324	6	4	theme	bacteria	897:904	arg1	impact					883:888	the impact	879:888	the impact of gut bacteria on the well-being of humans	879:932	This chapter summarizes the impact of gut bacteria on the well-being of humans and highlights the protective role played by the human microbiota during bacterial and viral infections.					
36280324	3	5	theme	genes	499:503	arg1	collection					475:484	the collection	471:484	the collection of microbial genes and genomes inhabiting the human body	471:541	The development of "omics" technologies gave way to the Human Microbiome Project (HMP) which aimed at exploring the collection of microbial genes and genomes inhabiting the human body.					
36280324	8	6	theme	CRISPR/Cas	1395:1404	arg1	system					1406:1411	CRISPR/Cas system	1395:1411	CRISPR/Cas system	1395:1411	Further, treatments such as fecal transplantation, probiotics, prebiotics, phage therapy, and CRISPR/Cas system, which target gut microbiota during digestive diseases are also discussed.					
36280324	7	7	theme	disorders	1147:1155	arg1	establishment					1097:1109	the establishment	1093:1109	the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity	1093:1274	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	8	theme	metabolic	1137:1145	arg1	disease					1226:1232	inflammatory bowel disease	1207:1232	inflammatory bowel disease (IBD)	1207:1238	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	8	theme	metabolic	1137:1145	arg1	disorders					1147:1155	metabolic disorders	1137:1155	metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity	1137:1274	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	8	theme	metabolic	1137:1145	arg1	periodontitis					1249:1261	periodontitis	1249:1261	periodontitis	1249:1261	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	8	theme	metabolic	1137:1145	arg1	obesity					1268:1274	obesity	1268:1274	obesity	1268:1274	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	8	theme	metabolic	1137:1145	arg1	cancer					1241:1246	cancer	1241:1246	cancer	1241:1246	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	8	theme	metabolic	1137:1145	arg1	infection					1190:1198	Clostridioides difficile infection	1165:1198	Clostridioides difficile infection (CFI)	1165:1204	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	5	9	theme	microorganisms	769:782	arg1	diversity					756:764	the diversity	752:764	the diversity of microorganisms	752:782	Whereas, an imbalance in the diversity of microorganisms, termed dysbiosis, greatly influences the state of health and disease.					
36280324	5	10	from	imbalance	739:747	arg1	diversity					756:764	the diversity	752:764	the diversity of microorganisms	752:782	Whereas, an imbalance in the diversity of microorganisms, termed dysbiosis, greatly influences the state of health and disease.					
36280324	6	11	theme	protective	953:962	arg1	role					964:967	the protective role	949:967	the protective role played by the human microbiota during bacterial and viral infections	949:1036	This chapter summarizes the impact of gut bacteria on the well-being of humans and highlights the protective role played by the human microbiota during bacterial and viral infections.					
36280324	3	12	theme	"	384:384	arg1	technologies					386:397	"omics" technologies	378:397	"omics" technologies	378:397	The development of "omics" technologies gave way to the Human Microbiome Project (HMP) which aimed at exploring the collection of microbial genes and genomes inhabiting the human body.					
36280324	6	13	theme	gut	893:895	arg1	bacteria					897:904	gut bacteria	893:904	gut bacteria	893:904	This chapter summarizes the impact of gut bacteria on the well-being of humans and highlights the protective role played by the human microbiota during bacterial and viral infections.					
36280324	7	14	theme	dysbiosis	1056:1064	arg1	condition					1043:1051	The condition	1039:1051	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity	1039:1274	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	15	theme	inflammatory	1207:1218	arg1	disease					1226:1232	inflammatory bowel disease	1207:1232	inflammatory bowel disease (IBD)	1207:1238	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	15	theme	inflammatory	1207:1218	arg1	IBD					1235:1237	IBD	1235:1237	IBD	1235:1237	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	5	16	theme	disease	846:852	arg1	state					826:830	the state	822:830	the state of health and disease	822:852	Whereas, an imbalance in the diversity of microorganisms, termed dysbiosis, greatly influences the state of health and disease.					
36280324	3	17	theme	technologies	386:397	arg1	development					363:373	The development	359:373	The development of "omics" technologies	359:397	The development of "omics" technologies gave way to the Human Microbiome Project (HMP) which aimed at exploring the collection of microbial genes and genomes inhabiting the human body.					
36280324	7	18	theme	plays	1077:1081	arg1	condition					1043:1051	The condition	1039:1051	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity	1039:1274	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	6	19	theme	human	983:987	arg1	microbiota					989:998	the human microbiota	979:998	the human microbiota	979:998	This chapter summarizes the impact of gut bacteria on the well-being of humans and highlights the protective role played by the human microbiota during bacterial and viral infections.					
36280324	1	20	theme	human	72:76	arg1	body					78:81	the human body	68:81	the human body	68:81	Since birth, the human body gets colonized by various communities of symbiotic or commensal microorganisms and they persist till the death of an individual.					
36280324	7	21	theme	difficile	1180:1188	arg1	CFI					1201:1203	CFI	1201:1203	CFI	1201:1203	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	21	theme	difficile	1180:1188	arg1	infection					1190:1198	Clostridioides difficile infection	1165:1198	Clostridioides difficile infection (CFI)	1165:1204	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	22	theme	bowel	1220:1224	arg1	disease					1226:1232	inflammatory bowel disease	1207:1232	inflammatory bowel disease (IBD)	1207:1238	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	22	theme	bowel	1220:1224	arg1	IBD					1235:1237	IBD	1235:1237	IBD	1235:1237	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	0	23	theme	microbiome	19:28	arg1	diseases					45:52	human microbiome and infectious diseases	13:52	human microbiome and infectious diseases	13:52	Dysbiosis of human microbiome and infectious diseases.					
36280324	8	24	theme	fecal	1329:1333	arg1	transplantation					1335:1349	fecal transplantation	1329:1349	fecal transplantation	1329:1349	Further, treatments such as fecal transplantation, probiotics, prebiotics, phage therapy, and CRISPR/Cas system, which target gut microbiota during digestive diseases are also discussed.					
36280324	1	25	dep	persist	171:177	arg1	till					179:182	till	179:182	till	179:182	Since birth, the human body gets colonized by various communities of symbiotic or commensal microorganisms and they persist till the death of an individual.					
36280324	0	26	theme	human	13:17	arg1	diseases					45:52	human microbiome and infectious diseases	13:52	human microbiome and infectious diseases	13:52	Dysbiosis of human microbiome and infectious diseases.					
36280324	3	27	theme	microbial	489:497	arg1	genes					499:503	microbial genes	489:503	microbial genes	489:503	The development of "omics" technologies gave way to the Human Microbiome Project (HMP) which aimed at exploring the collection of microbial genes and genomes inhabiting the human body.					
36280324	1	28	theme	symbiotic	124:132	arg1	microorganisms					147:160	symbiotic or commensal microorganisms	124:160	symbiotic or commensal microorganisms	124:160	Since birth, the human body gets colonized by various communities of symbiotic or commensal microorganisms and they persist till the death of an individual.					
36280324	0	29	theme	infectious	34:43	arg1	diseases					45:52	human microbiome and infectious diseases	13:52	human microbiome and infectious diseases	13:52	Dysbiosis of human microbiome and infectious diseases.					
36280324	8	30	theme	gut	1427:1429	arg1	microbiota					1431:1440	gut microbiota	1427:1440	gut microbiota	1427:1440	Further, treatments such as fecal transplantation, probiotics, prebiotics, phage therapy, and CRISPR/Cas system, which target gut microbiota during digestive diseases are also discussed.					
36280324	2	31	theme	human	216:220	arg1	microbiome					222:231	The human microbiome	212:231	The human microbiome	212:231	The human microbiome is comprised of the genomes of microorganisms such as viruses, archaea, eukaryotes, protozoa, and, most remarkably, bacteria.					
36280324	4	32	theme	healthy	562:568	arg1	composition					583:593	a healthy and balanced composition	560:593	a healthy and balanced composition of such microbes	560:610	Eubiosis, i.e., a healthy and balanced composition of such microbes contributes to the metabolic function, protection against pathogens and provides nutrients and energy to the host.					
36280324	4	33	theme	balanced	574:581	arg1	composition					583:593	a healthy and balanced composition	560:593	a healthy and balanced composition of such microbes	560:610	Eubiosis, i.e., a healthy and balanced composition of such microbes contributes to the metabolic function, protection against pathogens and provides nutrients and energy to the host.					
36280324	8	34	theme	digestive	1449:1457	arg1	diseases					1459:1466	digestive diseases	1449:1466	digestive diseases	1449:1466	Further, treatments such as fecal transplantation, probiotics, prebiotics, phage therapy, and CRISPR/Cas system, which target gut microbiota during digestive diseases are also discussed.					
36280324	6	35	from	impact	883:888	arg1	well-being					913:922	the well-being	909:922	the well-being of humans	909:932	This chapter summarizes the impact of gut bacteria on the well-being of humans and highlights the protective role played by the human microbiota during bacterial and viral infections.					
36280324	3	36	theme	human	532:536	arg1	body					538:541	the human body	528:541	the human body	528:541	The development of "omics" technologies gave way to the Human Microbiome Project (HMP) which aimed at exploring the collection of microbial genes and genomes inhabiting the human body.					
36280324	7	37	from	establishment	1097:1109	arg1	condition					1043:1051	The condition	1039:1051	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity	1039:1274	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	3	38	theme	genomes	509:515	arg1	collection					475:484	the collection	471:484	the collection of microbial genes and genomes inhabiting the human body	471:541	The development of "omics" technologies gave way to the Human Microbiome Project (HMP) which aimed at exploring the collection of microbial genes and genomes inhabiting the human body.					
36280324	4	39	theme	metabolic	631:639	arg1	function					641:648	the metabolic function	627:648	the metabolic function	627:648	Eubiosis, i.e., a healthy and balanced composition of such microbes contributes to the metabolic function, protection against pathogens and provides nutrients and energy to the host.					
36280324	0	40	theme	diseases	45:52	arg1	Dysbiosis					0:8	Dysbiosis	0:8	Dysbiosis of human microbiome and infectious diseases.	0:53	Dysbiosis of human microbiome and infectious diseases.					
36280324	8	41	theme	phage	1376:1380	arg1	therapy					1382:1388	phage therapy	1376:1388	phage therapy	1376:1388	Further, treatments such as fecal transplantation, probiotics, prebiotics, phage therapy, and CRISPR/Cas system, which target gut microbiota during digestive diseases are also discussed.					
36280324	1	42	theme	various	101:107	arg1	communities					109:119	various communities	101:119	various communities of symbiotic or commensal microorganisms	101:160	Since birth, the human body gets colonized by various communities of symbiotic or commensal microorganisms and they persist till the death of an individual.					
36280324	6	43	theme	humans	927:932	arg1	well-being					913:922	the well-being	909:922	the well-being of humans	909:932	This chapter summarizes the impact of gut bacteria on the well-being of humans and highlights the protective role played by the human microbiota during bacterial and viral infections.					
36280324	3	44	theme	Human	415:419	arg1	Project					432:438	the Human Microbiome Project	411:438	the Human Microbiome Project (HMP) which aimed at exploring the collection of microbial genes and genomes inhabiting the human body	411:541	The development of "omics" technologies gave way to the Human Microbiome Project (HMP) which aimed at exploring the collection of microbial genes and genomes inhabiting the human body.					
36280324	3	44	theme	Human	415:419	arg1	HMP					441:443	HMP	441:443	HMP	441:443	The development of "omics" technologies gave way to the Human Microbiome Project (HMP) which aimed at exploring the collection of microbial genes and genomes inhabiting the human body.					
36280324	6	45	theme	viral	1021:1025	arg1	infections					1027:1036	bacterial and viral infections	1007:1036	bacterial and viral infections	1007:1036	This chapter summarizes the impact of gut bacteria on the well-being of humans and highlights the protective role played by the human microbiota during bacterial and viral infections.					
36280324	1	46	theme	individual	200:209	arg1	death					188:192	the death	184:192	the death of an individual	184:209	Since birth, the human body gets colonized by various communities of symbiotic or commensal microorganisms and they persist till the death of an individual.					
36280324	3	47	theme	Microbiome	421:430	arg1	Project					432:438	the Human Microbiome Project	411:438	the Human Microbiome Project (HMP) which aimed at exploring the collection of microbial genes and genomes inhabiting the human body	411:541	The development of "omics" technologies gave way to the Human Microbiome Project (HMP) which aimed at exploring the collection of microbial genes and genomes inhabiting the human body.					
36280324	3	47	theme	Microbiome	421:430	arg1	HMP					441:443	HMP	441:443	HMP	441:443	The development of "omics" technologies gave way to the Human Microbiome Project (HMP) which aimed at exploring the collection of microbial genes and genomes inhabiting the human body.					
36280324	5	48	theme	health	835:840	arg1	state					826:830	the state	822:830	the state of health and disease	822:852	Whereas, an imbalance in the diversity of microorganisms, termed dysbiosis, greatly influences the state of health and disease.					
36280324	7	49	theme	infections	1122:1131	arg1	establishment					1097:1109	the establishment	1093:1109	the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity	1093:1274	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	6	50	theme	bacterial	1007:1015	arg1	infections					1027:1036	bacterial and viral infections	1007:1036	bacterial and viral infections	1007:1036	This chapter summarizes the impact of gut bacteria on the well-being of humans and highlights the protective role played by the human microbiota during bacterial and viral infections.					
36280324	4	51	theme	microbes	603:610	arg1	composition					583:593	a healthy and balanced composition	560:593	a healthy and balanced composition of such microbes	560:610	Eubiosis, i.e., a healthy and balanced composition of such microbes contributes to the metabolic function, protection against pathogens and provides nutrients and energy to the host.					
36280324	7	52	from	condition	1043:1051	arg1	establishment					1097:1109	the establishment	1093:1109	the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity	1093:1274	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	53	theme	Clostridioides	1165:1178	arg1	CFI					1201:1203	CFI	1201:1203	CFI	1201:1203	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	7	53	theme	Clostridioides	1165:1178	arg1	infection					1190:1198	Clostridioides difficile infection	1165:1198	Clostridioides difficile infection (CFI)	1165:1204	The condition of dysbiosis and how it plays a role in the establishment of various infections and metabolic disorders such as Clostridioides difficile infection (CFI), inflammatory bowel disease (IBD), cancer, periodontitis, and obesity are described in detail.					
36280324	4	54	theme	such	598:601	arg1	microbes					603:610	such microbes	598:610	such microbes	598:610	Eubiosis, i.e., a healthy and balanced composition of such microbes contributes to the metabolic function, protection against pathogens and provides nutrients and energy to the host.					
37059517	0	0	theme	composite	106:114	arg1	films					116:120	polyvinyl alcohol/quaternary chitosan composite films	68:120	polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging	68:148	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	3	1	theme	X-ray	719:723	arg1	spectra					758:764	X-ray photoelectron spectroscopy (XPS) spectra	719:764	X-ray photoelectron spectroscopy (XPS) spectra	719:764	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	2	2	theme	long-chain	377:386	arg1	contents					408:415	different long-chain alkyl and different contents	367:415	different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties	367:593	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	0	3	theme	chitosan	97:104	arg1	films					116:120	polyvinyl alcohol/quaternary chitosan composite films	68:120	polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging	68:148	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	5	4	theme	contents	1154:1161	arg1	increase					1118:1125	the increase	1114:1125	the increase of the quaternary chitosan contents	1114:1161	Optical properties demonstrated that the light transmittance on both UV and visible light showed a decreasing trend with the increase of the quaternary chitosan contents.					
37059517	2	5	theme	different	367:375	arg1	contents					408:415	different long-chain alkyl and different contents	367:415	different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties	367:593	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	8	6	theme	life	1514:1517	arg1	shelf					1505:1509	the shelf	1501:1509	the shelf of life on antibacterial packaging	1501:1544	This research demonstrated that the modified composite films could extend the shelf of life on antibacterial packaging.					
37059517	7	7	contain	had	1269:1271	arg2	properties					1291:1300	higher mechanical properties	1273:1300	higher mechanical properties	1273:1300	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	7	7	contain	had	1269:1271	arg1	films					1263:1267	the composite films	1249:1267	the composite films	1249:1267	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	2	8	theme	antibacterial	307:319	arg1	film					321:324	antibacterial film	307:324	antibacterial film	307:324	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	6	9	theme	Whereas	1164:1170	arg1	films					1186:1190	Whereas the composite films	1164:1190	Whereas the composite films	1164:1190	Whereas the composite films have enhanced hydrophobicity than PVA film.					
37059517	4	10	theme	stronger	960:967	arg1	properties					981:990	stronger antioxidant properties	960:990	stronger antioxidant properties	960:990	Besides, the modified films have better antibacterial effects against Escherichia (E. coli) and Staphylococcus (S. aureus) and present stronger antioxidant properties.					
37059517	0	11	theme	films	116:120	arg1	properties					54:63	antibacterial and mechanical properties	25:63	antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging	25:148	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	0	11	theme	films	116:120	arg1	hydrophobicity					9:22	Improved hydrophobicity	0:22	Improved hydrophobicity	0:22	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	7	12	theme	higher	1273:1278	arg1	properties					1291:1300	higher mechanical properties	1273:1300	higher mechanical properties	1273:1300	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	4	13	theme	antioxidant	969:979	arg1	properties					981:990	stronger antioxidant properties	960:990	stronger antioxidant properties	960:990	Besides, the modified films have better antibacterial effects against Escherichia (E. coli) and Staphylococcus (S. aureus) and present stronger antioxidant properties.					
37059517	3	14	theme	XPS	753:755	arg1	spectra					758:764	X-ray photoelectron spectroscopy (XPS) spectra	719:764	X-ray photoelectron spectroscopy (XPS) spectra	719:764	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	4	15	theme	better	858:863	arg1	effects					879:885	better antibacterial effects	858:885	better antibacterial effects against Escherichia (E. coli) and Staphylococcus (S. aureus)	858:946	Besides, the modified films have better antibacterial effects against Escherichia (E. coli) and Staphylococcus (S. aureus) and present stronger antioxidant properties.					
37059517	2	16	theme	solution	448:455	arg1	casting					457:463	solution casting	448:463	solution casting	448:463	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	5	17	theme	Optical	993:999	arg1	properties					1001:1010	Optical properties	993:1010	Optical properties	993:1010	Optical properties demonstrated that the light transmittance on both UV and visible light showed a decreasing trend with the increase of the quaternary chitosan contents.					
37059517	7	18	from	break	1365:1369	arg1	modulus					1320:1326	Young's modulus	1312:1326	Young's modulus	1312:1326	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	7	18	from	break	1365:1369	arg1	strength					1337:1344	tensile strength	1329:1344	tensile strength	1329:1344	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	7	18	from	break	1365:1369	arg1	elongation					1351:1360	elongation	1351:1360	elongation at break	1351:1369	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	2	19	theme	quaternary	420:429	arg1	chitosan					431:438	quaternary chitosan	420:438	quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties	420:593	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	0	20	theme	antibacterial	126:138	arg1	packaging					140:148	antibacterial packaging	126:148	antibacterial packaging	126:148	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	3	21	theme	photoelectron	725:737	arg1	spectra					758:764	X-ray photoelectron spectroscopy (XPS) spectra	719:764	X-ray photoelectron spectroscopy (XPS) spectra	719:764	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	7	22	theme	tensile	1329:1335	arg1	strength					1337:1344	tensile strength	1329:1344	tensile strength	1329:1344	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	3	23	dep	1470 cm-1	621:629	arg1	Transform					634:642	Transform	634:642	Transform Infrared Spectroscopy (FTIR)	634:671	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	2	24	theme	quaternary	475:484	arg1	properties					584:593	mechanical properties	573:593	mechanical properties	573:593	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	2	24	theme	quaternary	475:484	arg1	agent					530:534	an antibacterial agent	513:534	an antibacterial agent	513:534	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	2	24	theme	quaternary	475:484	arg1	chitosan					486:493	quaternary chitosan	475:493	quaternary chitosan	475:493	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	2	24	theme	quaternary	475:484	arg1	hydrophobicity					554:567	hydrophobicity	554:567	hydrophobicity	554:567	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	3	25	theme	spectroscopy	739:750	arg1	spectra					758:764	X-ray photoelectron spectroscopy (XPS) spectra	719:764	X-ray photoelectron spectroscopy (XPS) spectra	719:764	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	4	26	dep	Staphylococcus	921:934	arg1	aureus					940:945	S. aureus	937:945	S. aureus	937:945	Besides, the modified films have better antibacterial effects against Escherichia (E. coli) and Staphylococcus (S. aureus) and present stronger antioxidant properties.					
37059517	7	27	from	344.99 MPa	1376:1385	arg1	properties					1291:1300	higher mechanical properties	1273:1300	higher mechanical properties	1273:1300	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	0	28	theme	Improved	0:7	arg1	properties					54:63	antibacterial and mechanical properties	25:63	antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging	25:148	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	0	28	theme	Improved	0:7	arg1	hydrophobicity					9:22	Improved hydrophobicity	0:22	Improved hydrophobicity	0:22	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	4	29	theme	antibacterial	865:877	arg1	effects					879:885	better antibacterial effects	858:885	better antibacterial effects against Escherichia (E. coli) and Staphylococcus (S. aureus)	858:946	Besides, the modified films have better antibacterial effects against Escherichia (E. coli) and Staphylococcus (S. aureus) and present stronger antioxidant properties.					
37059517	6	30	theme	composite	1176:1184	arg1	films					1186:1190	Whereas the composite films	1164:1190	Whereas the composite films	1164:1190	Whereas the composite films have enhanced hydrophobicity than PVA film.					
37059517	2	31	theme	different	398:406	arg1	contents					408:415	different long-chain alkyl and different contents	367:415	different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties	367:593	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	0	32	theme	antibacterial	25:37	arg1	properties					54:63	antibacterial and mechanical properties	25:63	antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging	25:148	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	0	32	theme	antibacterial	25:37	arg1	hydrophobicity					9:22	Improved hydrophobicity	0:22	Improved hydrophobicity	0:22	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	8	33	theme	modified	1463:1470	arg1	films					1482:1486	the modified composite films	1459:1486	the modified composite films	1459:1486	This research demonstrated that the modified composite films could extend the shelf of life on antibacterial packaging.					
37059517	7	34	theme	mechanical	1280:1289	arg1	properties					1291:1300	higher mechanical properties	1273:1300	higher mechanical properties	1273:1300	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	7	35	from	39.12 MPa	1388:1396	arg1	properties					1291:1300	higher mechanical properties	1273:1300	higher mechanical properties	1273:1300	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	1	36	theme	attractive	197:206	arg1	alcohol					161:167	Polyvinyl alcohol	151:167	Polyvinyl alcohol (PVA)	151:173	Polyvinyl alcohol (PVA) and chitosan (CS) are attractive polymeric feedstocks for developing eco-environmental materials.					
37059517	1	36	theme	attractive	197:206	arg1	feedstocks					218:227	attractive polymeric feedstocks	197:227	attractive polymeric feedstocks for developing eco-environmental materials	197:270	Polyvinyl alcohol (PVA) and chitosan (CS) are attractive polymeric feedstocks for developing eco-environmental materials.					
37059517	1	36	theme	attractive	197:206	arg1	chitosan					179:186	chitosan	179:186	chitosan (CS)	179:191	Polyvinyl alcohol (PVA) and chitosan (CS) are attractive polymeric feedstocks for developing eco-environmental materials.					
37059517	8	37	from	shelf	1505:1509	arg1	packaging					1536:1544	antibacterial packaging	1522:1544	antibacterial packaging	1522:1544	This research demonstrated that the modified composite films could extend the shelf of life on antibacterial packaging.					
37059517	5	38	theme	chitosan	1145:1152	arg1	contents					1154:1161	the quaternary chitosan contents	1130:1161	the quaternary chitosan contents	1130:1161	Optical properties demonstrated that the light transmittance on both UV and visible light showed a decreasing trend with the increase of the quaternary chitosan contents.					
37059517	6	39	theme	enhanced	1197:1204	arg1	hydrophobicity					1206:1219	enhanced hydrophobicity	1197:1219	enhanced hydrophobicity	1197:1219	Whereas the composite films have enhanced hydrophobicity than PVA film.					
37059517	3	40	theme	novel	598:602	arg1	peak					604:607	A novel peak	596:607	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR)	596:671	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	5	41	from	transmittance	1040:1052	arg1	light					1077:1081	both UV and visible light	1057:1081	light	1077:1081	Optical properties demonstrated that the light transmittance on both UV and visible light showed a decreasing trend with the increase of the quaternary chitosan contents.					
37059517	5	41	from	transmittance	1040:1052	arg1	UV					1062:1063	both UV and visible light	1057:1081	UV	1062:1063	Optical properties demonstrated that the light transmittance on both UV and visible light showed a decreasing trend with the increase of the quaternary chitosan contents.					
37059517	1	42	theme	polymeric	208:216	arg1	alcohol					161:167	Polyvinyl alcohol	151:167	Polyvinyl alcohol (PVA)	151:173	Polyvinyl alcohol (PVA) and chitosan (CS) are attractive polymeric feedstocks for developing eco-environmental materials.					
37059517	1	42	theme	polymeric	208:216	arg1	feedstocks					218:227	attractive polymeric feedstocks	197:227	attractive polymeric feedstocks for developing eco-environmental materials	197:270	Polyvinyl alcohol (PVA) and chitosan (CS) are attractive polymeric feedstocks for developing eco-environmental materials.					
37059517	1	42	theme	polymeric	208:216	arg1	chitosan					179:186	chitosan	179:186	chitosan (CS)	179:191	Polyvinyl alcohol (PVA) and chitosan (CS) are attractive polymeric feedstocks for developing eco-environmental materials.					
37059517	0	43	theme	mechanical	43:52	arg1	properties					54:63	antibacterial and mechanical properties	25:63	antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging	25:148	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	0	43	theme	mechanical	43:52	arg1	hydrophobicity					9:22	Improved hydrophobicity	0:22	Improved hydrophobicity	0:22	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	7	44	theme	Young	1312:1316	arg1	modulus					1320:1326	Young's modulus	1312:1326	Young's modulus	1312:1326	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	2	45	theme	mechanical	573:582	arg1	properties					584:593	mechanical properties	573:593	mechanical properties	573:593	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	2	45	theme	mechanical	573:582	arg1	agent					530:534	an antibacterial agent	513:534	an antibacterial agent	513:534	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	2	45	theme	mechanical	573:582	arg1	chitosan					486:493	quaternary chitosan	475:493	quaternary chitosan	475:493	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	2	45	theme	mechanical	573:582	arg1	hydrophobicity					554:567	hydrophobicity	554:567	hydrophobicity	554:567	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	3	46	theme	new	679:681	arg1	peak					701:704	a new CCl bond spectral peak	677:704	a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra	677:764	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	3	47	from	200 eV	709:714	arg1	peak					604:607	A novel peak	596:607	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR)	596:671	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	3	47	from	200 eV	709:714	arg1	spectra					758:764	X-ray photoelectron spectroscopy (XPS) spectra	719:764	X-ray photoelectron spectroscopy (XPS) spectra	719:764	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	3	47	from	200 eV	709:714	arg1	peak					701:704	a new CCl bond spectral peak	677:704	a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra	677:764	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	8	48	theme	composite	1472:1480	arg1	films					1482:1486	the modified composite films	1459:1486	the modified composite films	1459:1486	This research demonstrated that the modified composite films could extend the shelf of life on antibacterial packaging.					
37059517	2	49	theme	chitosan	431:438	arg1	contents					408:415	different long-chain alkyl and different contents	367:415	different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties	367:593	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	4	50	dep	Escherichia	895:905	arg1	coli					911:914	E. coli	908:914	E. coli	908:914	Besides, the modified films have better antibacterial effects against Escherichia (E. coli) and Staphylococcus (S. aureus) and present stronger antioxidant properties.					
37059517	0	51	theme	polyvinyl	68:76	arg1	films					116:120	polyvinyl alcohol/quaternary chitosan composite films	68:120	polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging	68:148	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	7	52	theme	composite	1253:1261	arg1	films					1263:1267	the composite films	1249:1267	the composite films	1249:1267	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	3	53	theme	spectral	692:699	arg1	peak					701:704	a new CCl bond spectral peak	677:704	a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra	677:764	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	4	54	theme	modified	838:845	arg1	films					847:851	the modified films	834:851	the modified films	834:851	Besides, the modified films have better antibacterial effects against Escherichia (E. coli) and Staphylococcus (S. aureus) and present stronger antioxidant properties.					
37059517	1	55	theme	Polyvinyl	151:159	arg1	alcohol					161:167	Polyvinyl alcohol	151:167	Polyvinyl alcohol (PVA)	151:173	Polyvinyl alcohol (PVA) and chitosan (CS) are attractive polymeric feedstocks for developing eco-environmental materials.					
37059517	1	55	theme	Polyvinyl	151:159	arg1	feedstocks					218:227	attractive polymeric feedstocks	197:227	attractive polymeric feedstocks for developing eco-environmental materials	197:270	Polyvinyl alcohol (PVA) and chitosan (CS) are attractive polymeric feedstocks for developing eco-environmental materials.					
37059517	1	55	theme	Polyvinyl	151:159	arg1	PVA					170:172	PVA	170:172	PVA	170:172	Polyvinyl alcohol (PVA) and chitosan (CS) are attractive polymeric feedstocks for developing eco-environmental materials.					
37059517	1	55	theme	Polyvinyl	151:159	arg1	chitosan					179:186	chitosan	179:186	chitosan (CS)	179:191	Polyvinyl alcohol (PVA) and chitosan (CS) are attractive polymeric feedstocks for developing eco-environmental materials.					
37059517	6	56	theme	PVA	1226:1228	arg1	film					1230:1233	PVA film	1226:1233	PVA film	1226:1233	Whereas the composite films have enhanced hydrophobicity than PVA film.					
37059517	5	57	theme	visible	1069:1075	arg1	light					1077:1081	both UV and visible light	1057:1081	light	1077:1081	Optical properties demonstrated that the light transmittance on both UV and visible light showed a decreasing trend with the increase of the quaternary chitosan contents.					
37059517	2	58	theme	PVA	349:351	arg1	blending					353:360	PVA blending	349:360	PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties	349:593	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	7	59	from	%	1410:1410	arg1	properties					1291:1300	higher mechanical properties	1273:1300	higher mechanical properties	1273:1300	Furthermore, the composite films had higher mechanical properties, in which Young's modulus, tensile strength, and elongation at break were 344.99 MPa, 39.12 MPa, and 507.09 %, respectively.					
37059517	6	60	contain	have	1192:1195	arg2	hydrophobicity					1206:1219	enhanced hydrophobicity	1197:1219	enhanced hydrophobicity	1197:1219	Whereas the composite films have enhanced hydrophobicity than PVA film.					
37059517	6	60	contain	have	1192:1195	arg1	films					1186:1190	Whereas the composite films	1164:1190	Whereas the composite films	1164:1190	Whereas the composite films have enhanced hydrophobicity than PVA film.					
37059517	8	61	theme	antibacterial	1522:1534	arg1	packaging					1536:1544	antibacterial packaging	1522:1544	antibacterial packaging	1522:1544	This research demonstrated that the modified composite films could extend the shelf of life on antibacterial packaging.					
37059517	3	62	theme	CCl	683:685	arg1	peak					701:704	a new CCl bond spectral peak	677:704	a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra	677:764	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	5	63	theme	decreasing	1092:1101	arg1	trend					1103:1107	a decreasing trend	1090:1107	a decreasing trend	1090:1107	Optical properties demonstrated that the light transmittance on both UV and visible light showed a decreasing trend with the increase of the quaternary chitosan contents.					
37059517	4	64	contain	have	853:856	arg2	effects					879:885	better antibacterial effects	858:885	better antibacterial effects against Escherichia (E. coli) and Staphylococcus (S. aureus)	858:946	Besides, the modified films have better antibacterial effects against Escherichia (E. coli) and Staphylococcus (S. aureus) and present stronger antioxidant properties.					
37059517	4	64	contain	have	853:856	arg1	films					847:851	the modified films	834:851	the modified films	834:851	Besides, the modified films have better antibacterial effects against Escherichia (E. coli) and Staphylococcus (S. aureus) and present stronger antioxidant properties.					
37059517	1	65	theme	eco-environmental	244:260	arg1	materials					262:270	eco-environmental materials	244:270	eco-environmental materials	244:270	Polyvinyl alcohol (PVA) and chitosan (CS) are attractive polymeric feedstocks for developing eco-environmental materials.					
37059517	0	66	theme	alcohol/quaternary	78:95	arg1	films					116:120	polyvinyl alcohol/quaternary chitosan composite films	68:120	polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging	68:148	Improved hydrophobicity, antibacterial and mechanical properties of polyvinyl alcohol/quaternary chitosan composite films for antibacterial packaging.					
37059517	3	67	theme	bond	687:690	arg1	peak					701:704	a new CCl bond spectral peak	677:704	a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra	677:764	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	3	68	theme	Infrared	644:651	arg1	Spectroscopy					653:664	Infrared Spectroscopy	644:664	Infrared Spectroscopy (FTIR)	644:671	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	3	68	theme	Infrared	644:651	arg1	FTIR					667:670	FTIR	667:670	FTIR	667:670	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	2	69	with	blending	353:360	arg1	contents					408:415	different long-chain alkyl and different contents	367:415	different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties	367:593	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	3	70	mod	modified	801:808	arg3	quaternary					813:822	quaternary	813:822	quaternary	813:822	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	3	70	mod	modified	801:808	arg1	CS					781:782	CS	781:782	CS	781:782	A novel peak appeared at 1470 cm-1 in Transform Infrared Spectroscopy (FTIR) and a new CCl bond spectral peak at 200 eV in X-ray photoelectron spectroscopy (XPS) spectra suggested that CS was successfully modified by quaternary.					
37059517	2	71	theme	antibacterial	516:528	arg1	properties					584:593	mechanical properties	573:593	mechanical properties	573:593	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	2	71	theme	antibacterial	516:528	arg1	agent					530:534	an antibacterial agent	513:534	an antibacterial agent	513:534	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	2	71	theme	antibacterial	516:528	arg1	chitosan					486:493	quaternary chitosan	475:493	quaternary chitosan	475:493	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	2	71	theme	antibacterial	516:528	arg1	hydrophobicity					554:567	hydrophobicity	554:567	hydrophobicity	554:567	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
37059517	5	72	theme	quaternary	1134:1143	arg1	contents					1154:1161	the quaternary chitosan contents	1130:1161	the quaternary chitosan contents	1130:1161	Optical properties demonstrated that the light transmittance on both UV and visible light showed a decreasing trend with the increase of the quaternary chitosan contents.					
37059517	5	73	theme	light	1034:1038	arg1	transmittance					1040:1052	the light transmittance	1030:1052	the light transmittance on both UV and visible light	1030:1081	Optical properties demonstrated that the light transmittance on both UV and visible light showed a decreasing trend with the increase of the quaternary chitosan contents.					
37059517	2	74	theme	alkyl	388:392	arg1	contents					408:415	different long-chain alkyl and different contents	367:415	different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties	367:593	In this work, a biodegradable and antibacterial film was developed based on PVA blending with different long-chain alkyl and different contents of quaternary chitosan through solution casting, in which quaternary chitosan not only acted as an antibacterial agent but also improved hydrophobicity and mechanical properties.					
36593253	9	0	theme	degree	1030:1035	arg1	value					1037:1041	crystallinity degree value	1016:1041	crystallinity degree value of 79.58%	1016:1051	X-ray diffraction show that the obtained bacterial nanocellulose has crystallinity degree value of 79.58%.					
36593253	13	1	theme	bacterial	1408:1416	arg1	production was					1432:1445	maximum bacterial nanocellulose production was	1400:1445	maximum bacterial nanocellulose production was	1400:1445	Using the desirability function, the optimum conditions for maximum bacterial nanocellulose production was determined theoretically and verified experimentally.					
36593253	12	2	theme	bacterial	1225:1233	arg1	production					1249:1258	the bacterial nanocellulose production	1221:1258	the bacterial nanocellulose production	1221:1258	Optimization of the bacterial nanocellulose production was achieved using Plackett-Burman and face centered central composite designs.					
36593253	11	3	theme	thermogravimetric	1145:1161	arg1	analysis					1163:1170	The thermogravimetric analysis	1141:1170	The thermogravimetric analysis	1141:1170	The thermogravimetric analysis revealed high thermal stability.					
36593253	7	4	theme	fiber-shaped	791:802	arg1	particles					804:812	fiber-shaped particles	791:812	fiber-shaped particles	791:812	The SEM images of the bacterial nanocellulose confirms the formation of fiber-shaped particles with diameters of 20.12‒47.36 nm.					
36593253	15	5	theme	initial	1735:1741	arg1	level					1746:1750	an initial pH level	1732:1750	an initial pH level of 5	1732:1755	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	13	6	theme	nanocellulose	1418:1430	arg1	production was					1432:1445	maximum bacterial nanocellulose production was	1400:1445	maximum bacterial nanocellulose production was	1400:1445	Using the desirability function, the optimum conditions for maximum bacterial nanocellulose production was determined theoretically and verified experimentally.					
36593253	14	7	theme	Maximum	1501:1507	arg1	20.31 g/L					1525:1533	20.31 g/L	1525:1533	20.31 g/L	1525:1533	Maximum BNC production (20.31 g/L) by Bacillus sp.					
36593253	14	7	theme	Maximum	1501:1507	arg1	production					1513:1522	Maximum BNC production	1501:1522	Maximum BNC production (20.31 g/L)	1501:1534	Maximum BNC production (20.31 g/L) by Bacillus sp.					
36593253	1	8	theme	biomedical	167:176	arg1	carriers					201:208	carriers	201:208	carriers for drug delivery, blood vessels, artificial skin and wound dressing	201:277	The bacterial nanocellulose has been used in a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing.					
36593253	1	8	theme	biomedical	167:176	arg1	applications					178:189	biomedical applications	167:189	biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing	167:277	The bacterial nanocellulose has been used in a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing.					
36593253	1	9	theme	wound	264:268	arg1	dressing					270:277	wound dressing	264:277	wound dressing	264:277	The bacterial nanocellulose has been used in a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing.					
36593253	15	10	theme	percent	1798:1804	arg1	Na2HPO4					1716:1722	Na2HPO4	1716:1722	Na2HPO4	1716:1722	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	10	theme	percent	1798:1804	arg1	11.22 g/L					1818:1826	peptone 11.22 g/L	1810:1826	peptone 11.22 g/L	1810:1826	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	10	theme	percent	1798:1804	arg1	level					1746:1750	an initial pH level	1732:1750	an initial pH level of 5	1732:1755	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	10	theme	percent	1798:1804	arg1	acid					1659:1662	citric acid	1652:1662	citric acid	1652:1662	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	10	theme	percent	1798:1804	arg1	temperature					1696:1706	temperature	1696:1706	temperature	1696:1706	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	10	theme	percent	1798:1804	arg1	v/v					1647:1649	v/v	1647:1649	v/v	1647:1649	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	10	theme	percent	1798:1804	arg1	volume					1591:1596	medium volume	1584:1596	medium volume	1584:1596	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	10	theme	percent	1798:1804	arg1	extract					1680:1686	yeast extract	1674:1686	yeast extract	1674:1686	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	10	theme	percent	1798:1804	arg1	size					1637:1640	inoculum size	1628:1640	inoculum size	1628:1640	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	10	theme	percent	1798:1804	arg1	concentration					1775:1787	Cantaloupe juice concentration	1758:1787	Cantaloupe juice concentration of 81.27 percent	1758:1804	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	12	11	theme	production	1249:1258	arg1	Optimization					1205:1216	Optimization	1205:1216	Optimization of the bacterial nanocellulose production	1205:1258	Optimization of the bacterial nanocellulose production was achieved using Plackett-Burman and face centered central composite designs.					
36593253	8	12	theme	560‒1400 nm	934:944	arg1	diameters					897:905	diameters	897:905	diameters of 30‒40 nm	897:917	The TEM images show needle-shaped particles with diameters of 30‒40 nm and lengths of 560‒1400 nm.					
36593253	8	12	theme	560‒1400 nm	934:944	arg1	lengths					923:929	lengths	923:929	lengths of 560‒1400 nm	923:944	The TEM images show needle-shaped particles with diameters of 30‒40 nm and lengths of 560‒1400 nm.					
36593253	12	13	theme	composite	1321:1329	arg1	designs					1331:1337	central composite designs	1313:1337	central composite designs	1313:1337	Optimization of the bacterial nanocellulose production was achieved using Plackett-Burman and face centered central composite designs.					
36593253	12	14	theme	nanocellulose	1235:1247	arg1	production					1249:1258	the bacterial nanocellulose production	1221:1258	the bacterial nanocellulose production	1221:1258	Optimization of the bacterial nanocellulose production was achieved using Plackett-Burman and face centered central composite designs.					
36593253	15	15	theme	peptone	1810:1816	arg1	11.22 g/L					1818:1826	peptone 11.22 g/L	1810:1826	peptone 11.22 g/L	1810:1826	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	16	theme	strain	1552:1557	arg1	SEE-3					1559:1563	strain SEE-3	1552:1563	strain SEE-3	1552:1563	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	17	theme	medium	1584:1589	arg1	Na2HPO4					1716:1722	Na2HPO4	1716:1722	Na2HPO4	1716:1722	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	17	theme	medium	1584:1589	arg1	acid					1659:1662	citric acid	1652:1662	citric acid	1652:1662	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	17	theme	medium	1584:1589	arg1	temperature					1696:1706	temperature	1696:1706	temperature	1696:1706	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	17	theme	medium	1584:1589	arg1	v/v					1647:1649	v/v	1647:1649	v/v	1647:1649	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	17	theme	medium	1584:1589	arg1	volume					1591:1596	medium volume	1584:1596	medium volume	1584:1596	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	17	theme	medium	1584:1589	arg1	extract					1680:1686	yeast extract	1674:1686	yeast extract	1674:1686	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	17	theme	medium	1584:1589	arg1	size					1637:1640	inoculum size	1628:1640	inoculum size	1628:1640	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	9	18	theme	%	1051:1051	arg1	value					1037:1041	crystallinity degree value	1016:1041	crystallinity degree value of 79.58%	1016:1051	X-ray diffraction show that the obtained bacterial nanocellulose has crystallinity degree value of 79.58%.					
36593253	13	19	theme	optimum	1377:1383	arg1	conditions					1385:1394	the optimum conditions	1373:1394	the optimum conditions for maximum bacterial nanocellulose production was	1373:1445	Using the desirability function, the optimum conditions for maximum bacterial nanocellulose production was determined theoretically and verified experimentally.					
36593253	15	20	dep	volume	1591:1596	arg1	5 g/L					1689:1693	5 g/L	1689:1693	5 g/L	1689:1693	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	20	dep	volume	1591:1596	arg1	1.5 g/L					1665:1671	1.5 g/L	1665:1671	1.5 g/L	1665:1671	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	20	dep	volume	1591:1596	arg1	flask					1621:1625	100 mL/250 mL conical flask	1599:1625	100 mL/250 mL conical flask	1599:1625	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	20	dep	volume	1591:1596	arg1	%					1644:1644	5%	1643:1644	5%	1643:1644	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	20	dep	volume	1591:1596	arg1	3 g/L					1725:1729	3 g/L	1725:1729	medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L	1584:1729	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	20	dep	volume	1591:1596	arg1	37 °C					1709:1713	37 °C	1709:1713	37 °C	1709:1713	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	1	21	theme	applications	178:189	arg1	carriers					201:208	carriers	201:208	carriers for drug delivery, blood vessels, artificial skin and wound dressing	201:277	The bacterial nanocellulose has been used in a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing.					
36593253	1	21	theme	applications	178:189	arg1	range					158:162	a wide range	151:162	a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing	151:277	The bacterial nanocellulose has been used in a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing.					
36593253	15	22	theme	Cantaloupe	1758:1767	arg1	concentration					1775:1787	Cantaloupe juice concentration	1758:1787	Cantaloupe juice concentration of 81.27 percent	1758:1804	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	4	23	theme	bacterial	505:513	arg1	nanocellulose					515:527	the bacterial nanocellulose	501:527	the bacterial nanocellulose	501:527	strain SEE-3 exhibited potent ability to produce the bacterial nanocellulose.					
36593253	8	24	theme	needle-shaped	868:880	arg1	particles					882:890	needle-shaped particles	868:890	needle-shaped particles with diameters of 30‒40 nm and lengths of 560‒1400 nm	868:944	The TEM images show needle-shaped particles with diameters of 30‒40 nm and lengths of 560‒1400 nm.					
36593253	5	25	theme	biosynthesized	594:607	arg1	nanocellulose					609:621	the purified biosynthesized nanocellulose	581:621	the purified biosynthesized nanocellulose	581:621	The crystallinity, particle size and morphology of the purified biosynthesized nanocellulose were characterized.					
36593253	6	26	theme	cellulose	647:655	arg1	nanofibers					657:666	The cellulose nanofibers	643:666	The cellulose nanofibers	643:666	The cellulose nanofibers possess a negatively charged surface of - 14.7 mV.					
36593253	15	27	theme	citric	1652:1657	arg1	acid					1659:1662	citric acid	1652:1662	citric acid	1652:1662	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	27	theme	citric	1652:1657	arg1	volume					1591:1596	medium volume	1584:1596	medium volume	1584:1596	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	0	28	theme	nanocellulose	10:22	arg1	production					24:33	Bacterial nanocellulose production	0:33	Bacterial nanocellulose production	0:33	Bacterial nanocellulose production using Cantaloupe juice, statistical optimization and characterization.					
36593253	5	29	theme	nanocellulose	609:621	arg1	size					558:561	particle size	549:561	particle size	549:561	The crystallinity, particle size and morphology of the purified biosynthesized nanocellulose were characterized.					
36593253	5	29	theme	nanocellulose	609:621	arg1	morphology					567:576	morphology	567:576	morphology	567:576	The crystallinity, particle size and morphology of the purified biosynthesized nanocellulose were characterized.					
36593253	5	29	theme	nanocellulose	609:621	arg1	crystallinity					534:546	The crystallinity	530:546	The crystallinity	530:546	The crystallinity, particle size and morphology of the purified biosynthesized nanocellulose were characterized.					
36593253	2	30	theme	strains	333:339	arg1	total					284:288	The total	280:288	The total of ten morphologically different bacterial strains	280:339	The total of ten morphologically different bacterial strains were screened for their potential to produce bacterial nanocellulose (BNC).					
36593253	3	31	theme	Bacillus	439:446	arg1	sp					448:449	Bacillus sp	439:449	Bacillus sp	439:449	Among these isolates, Bacillus sp.					
36593253	0	32	theme	Bacterial	0:8	arg1	production					24:33	Bacterial nanocellulose production	0:33	Bacterial nanocellulose production	0:33	Bacterial nanocellulose production using Cantaloupe juice, statistical optimization and characterization.					
36593253	7	33	theme	nanocellulose	751:763	arg1	images					727:732	The SEM images	719:732	The SEM images of the bacterial nanocellulose	719:763	The SEM images of the bacterial nanocellulose confirms the formation of fiber-shaped particles with diameters of 20.12‒47.36 nm.					
36593253	15	34	theme	5	1755:1755	arg1	Na2HPO4					1716:1722	Na2HPO4	1716:1722	Na2HPO4	1716:1722	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	34	theme	5	1755:1755	arg1	11.22 g/L					1818:1826	peptone 11.22 g/L	1810:1826	peptone 11.22 g/L	1810:1826	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	34	theme	5	1755:1755	arg1	level					1746:1750	an initial pH level	1732:1750	an initial pH level of 5	1732:1755	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	34	theme	5	1755:1755	arg1	acid					1659:1662	citric acid	1652:1662	citric acid	1652:1662	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	34	theme	5	1755:1755	arg1	temperature					1696:1706	temperature	1696:1706	temperature	1696:1706	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	34	theme	5	1755:1755	arg1	v/v					1647:1649	v/v	1647:1649	v/v	1647:1649	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	34	theme	5	1755:1755	arg1	volume					1591:1596	medium volume	1584:1596	medium volume	1584:1596	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	34	theme	5	1755:1755	arg1	extract					1680:1686	yeast extract	1674:1686	yeast extract	1674:1686	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	34	theme	5	1755:1755	arg1	size					1637:1640	inoculum size	1628:1640	inoculum size	1628:1640	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	34	theme	5	1755:1755	arg1	concentration					1775:1787	Cantaloupe juice concentration	1758:1787	Cantaloupe juice concentration of 81.27 percent	1758:1804	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	1	35	theme	bacterial	110:118	arg1	nanocellulose					120:132	The bacterial nanocellulose	106:132	The bacterial nanocellulose	106:132	The bacterial nanocellulose has been used in a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing.					
36593253	12	36	theme	central	1313:1319	arg1	designs					1331:1337	central composite designs	1313:1337	central composite designs	1313:1337	Optimization of the bacterial nanocellulose production was achieved using Plackett-Burman and face centered central composite designs.					
36593253	9	37	theme	X-ray	947:951	arg1	diffraction					953:963	X-ray diffraction	947:963	X-ray diffraction	947:963	X-ray diffraction show that the obtained bacterial nanocellulose has crystallinity degree value of 79.58%.					
36593253	15	38	theme	81.27	1792:1796	arg1	percent					1798:1804	81.27 percent	1792:1804	81.27 percent	1792:1804	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	2	39	theme	different	313:321	arg1	strains					333:339	ten morphologically different bacterial strains	293:339	ten morphologically different bacterial strains	293:339	The total of ten morphologically different bacterial strains were screened for their potential to produce bacterial nanocellulose (BNC).					
36593253	1	40	theme	drug	214:217	arg1	delivery					219:226	drug delivery	214:226	drug delivery	214:226	The bacterial nanocellulose has been used in a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing.					
36593253	2	41	theme	bacterial	323:331	arg1	strains					333:339	ten morphologically different bacterial strains	293:339	ten morphologically different bacterial strains	293:339	The total of ten morphologically different bacterial strains were screened for their potential to produce bacterial nanocellulose (BNC).					
36593253	15	42	theme	inoculum	1628:1635	arg1	size					1637:1640	inoculum size	1628:1640	inoculum size	1628:1640	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	42	theme	inoculum	1628:1635	arg1	volume					1591:1596	medium volume	1584:1596	medium volume	1584:1596	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	14	43	theme	BNC	1509:1511	arg1	20.31 g/L					1525:1533	20.31 g/L	1525:1533	20.31 g/L	1525:1533	Maximum BNC production (20.31 g/L) by Bacillus sp.					
36593253	14	43	theme	BNC	1509:1511	arg1	production					1513:1522	Maximum BNC production	1501:1522	Maximum BNC production (20.31 g/L)	1501:1534	Maximum BNC production (20.31 g/L) by Bacillus sp.					
36593253	8	44	with	particles	882:890	arg1	diameters					897:905	diameters	897:905	diameters of 30‒40 nm	897:917	The TEM images show needle-shaped particles with diameters of 30‒40 nm and lengths of 560‒1400 nm.					
36593253	8	44	with	particles	882:890	arg1	lengths					923:929	lengths	923:929	lengths of 560‒1400 nm	923:944	The TEM images show needle-shaped particles with diameters of 30‒40 nm and lengths of 560‒1400 nm.					
36593253	8	45	theme	30‒40 nm	910:917	arg1	diameters					897:905	diameters	897:905	diameters of 30‒40 nm	897:917	The TEM images show needle-shaped particles with diameters of 30‒40 nm and lengths of 560‒1400 nm.					
36593253	8	45	theme	30‒40 nm	910:917	arg1	lengths					923:929	lengths	923:929	lengths of 560‒1400 nm	923:944	The TEM images show needle-shaped particles with diameters of 30‒40 nm and lengths of 560‒1400 nm.					
36593253	14	46	theme	Bacillus	1539:1546	arg1	sp					1548:1549	Bacillus sp	1539:1549	Bacillus sp	1539:1549	Maximum BNC production (20.31 g/L) by Bacillus sp.					
36593253	0	47	theme	Cantaloupe	41:50	arg1	juice					52:56	Cantaloupe juice	41:56	Cantaloupe juice	41:56	Bacterial nanocellulose production using Cantaloupe juice, statistical optimization and characterization.					
36593253	7	48	theme	particles	804:812	arg1	formation					778:786	the formation	774:786	the formation of fiber-shaped particles with diameters of 20.12‒47.36 nm	774:845	The SEM images of the bacterial nanocellulose confirms the formation of fiber-shaped particles with diameters of 20.12‒47.36 nm.					
36593253	7	49	theme	bacterial	741:749	arg1	nanocellulose					751:763	the bacterial nanocellulose	737:763	the bacterial nanocellulose	737:763	The SEM images of the bacterial nanocellulose confirms the formation of fiber-shaped particles with diameters of 20.12‒47.36 nm.					
36593253	3	50	dep	sp	448:449	arg1	isolates					429:436	these isolates	423:436	these isolates	423:436	Among these isolates, Bacillus sp.					
36593253	7	51	with	formation	778:786	arg1	diameters					819:827	diameters	819:827	diameters of 20.12‒47.36 nm	819:845	The SEM images of the bacterial nanocellulose confirms the formation of fiber-shaped particles with diameters of 20.12‒47.36 nm.					
36593253	1	52	theme	blood	229:233	arg1	vessels					235:241	blood vessels	229:241	blood vessels	229:241	The bacterial nanocellulose has been used in a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing.					
36593253	0	53	theme	statistical	59:69	arg1	optimization					71:82	statistical optimization	59:82	statistical optimization	59:82	Bacterial nanocellulose production using Cantaloupe juice, statistical optimization and characterization.					
36593253	6	54	contain	possess	668:674	arg2	of - 14.7 mV					705:716	a negatively charged surface of - 14.7 mV	676:716	a negatively charged surface of - 14.7 mV	676:716	The cellulose nanofibers possess a negatively charged surface of - 14.7 mV.					
36593253	6	54	contain	possess	668:674	arg1	nanofibers					657:666	The cellulose nanofibers	643:666	The cellulose nanofibers	643:666	The cellulose nanofibers possess a negatively charged surface of - 14.7 mV.					
36593253	4	55	theme	potent	475:480	arg1	ability					482:488	potent ability	475:488	potent ability to produce the bacterial nanocellulose	475:527	strain SEE-3 exhibited potent ability to produce the bacterial nanocellulose.					
36593253	13	56	theme	desirability	1350:1361	arg1	function					1363:1370	the desirability function	1346:1370	the desirability function	1346:1370	Using the desirability function, the optimum conditions for maximum bacterial nanocellulose production was determined theoretically and verified experimentally.					
36593253	15	57	theme	100 mL/250 mL	1599:1611	arg1	flask					1621:1625	100 mL/250 mL conical flask	1599:1625	100 mL/250 mL conical flask	1599:1625	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	9	58	theme	obtained	979:986	arg1	nanocellulose					998:1010	the obtained bacterial nanocellulose	975:1010	the obtained bacterial nanocellulose	975:1010	X-ray diffraction show that the obtained bacterial nanocellulose has crystallinity degree value of 79.58%.					
36593253	10	59	theme	FTIR	1054:1057	arg1	spectra					1059:1065	FTIR spectra	1054:1065	FTIR spectra	1054:1065	FTIR spectra revealed the characteristic bands of the cellulose crystalline structure.					
36593253	10	60	theme	crystalline structure	1118:1138	arg1	bands					1095:1099	the characteristic bands	1076:1099	the characteristic bands of the cellulose crystalline structure	1076:1138	FTIR spectra revealed the characteristic bands of the cellulose crystalline structure.					
36593253	10	61	theme	cellulose	1108:1116	arg1	crystalline structure					1118:1138	the cellulose crystalline structure	1104:1138	the cellulose crystalline structure	1104:1138	FTIR spectra revealed the characteristic bands of the cellulose crystalline structure.					
36593253	15	62	theme	conical	1613:1619	arg1	flask					1621:1625	100 mL/250 mL conical flask	1599:1625	100 mL/250 mL conical flask	1599:1625	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	9	63	theme	bacterial	988:996	arg1	nanocellulose					998:1010	the obtained bacterial nanocellulose	975:1010	the obtained bacterial nanocellulose	975:1010	X-ray diffraction show that the obtained bacterial nanocellulose has crystallinity degree value of 79.58%.					
36593253	14	64	dep	production	1513:1522	arg1	sp					1548:1549	Bacillus sp	1539:1549	Bacillus sp	1539:1549	Maximum BNC production (20.31 g/L) by Bacillus sp.					
36593253	5	65	theme	purified	585:592	arg1	nanocellulose					609:621	the purified biosynthesized nanocellulose	581:621	the purified biosynthesized nanocellulose	581:621	The crystallinity, particle size and morphology of the purified biosynthesized nanocellulose were characterized.					
36593253	1	66	used	used	143:146	arg2	nanocellulose					120:132	The bacterial nanocellulose	106:132	The bacterial nanocellulose	106:132	The bacterial nanocellulose has been used in a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing.					
36593253	9	67	contain	has	1012:1014	arg2	value					1037:1041	crystallinity degree value	1016:1041	crystallinity degree value of 79.58%	1016:1051	X-ray diffraction show that the obtained bacterial nanocellulose has crystallinity degree value of 79.58%.					
36593253	9	67	contain	has	1012:1014	arg1	nanocellulose					998:1010	the obtained bacterial nanocellulose	975:1010	the obtained bacterial nanocellulose	975:1010	X-ray diffraction show that the obtained bacterial nanocellulose has crystallinity degree value of 79.58%.					
36593253	7	68	theme	SEM	723:725	arg1	images					727:732	The SEM images	719:732	The SEM images of the bacterial nanocellulose	719:763	The SEM images of the bacterial nanocellulose confirms the formation of fiber-shaped particles with diameters of 20.12‒47.36 nm.					
36593253	15	69	theme	juice	1769:1773	arg1	concentration					1775:1787	Cantaloupe juice concentration	1758:1787	Cantaloupe juice concentration of 81.27 percent	1758:1804	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	11	70	theme	thermal	1186:1192	arg1	stability					1194:1202	thermal stability	1186:1202	thermal stability	1186:1202	The thermogravimetric analysis revealed high thermal stability.					
36593253	7	71	theme	20.12‒47.36 nm	832:845	arg1	diameters					819:827	diameters	819:827	diameters of 20.12‒47.36 nm	819:845	The SEM images of the bacterial nanocellulose confirms the formation of fiber-shaped particles with diameters of 20.12‒47.36 nm.					
36593253	15	72	theme	yeast	1674:1678	arg1	extract					1680:1686	yeast extract	1674:1686	yeast extract	1674:1686	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	15	72	theme	yeast	1674:1678	arg1	volume					1591:1596	medium volume	1584:1596	medium volume	1584:1596	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	5	73	theme	particle	549:556	arg1	size					558:561	particle size	549:561	particle size	549:561	The crystallinity, particle size and morphology of the purified biosynthesized nanocellulose were characterized.					
36593253	1	74	theme	wide	153:156	arg1	carriers					201:208	carriers	201:208	carriers for drug delivery, blood vessels, artificial skin and wound dressing	201:277	The bacterial nanocellulose has been used in a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing.					
36593253	1	74	theme	wide	153:156	arg1	range					158:162	a wide range	151:162	a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing	151:277	The bacterial nanocellulose has been used in a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing.					
36593253	6	75	theme	surface	697:703	arg1	of - 14.7 mV					705:716	a negatively charged surface of - 14.7 mV	676:716	a negatively charged surface of - 14.7 mV	676:716	The cellulose nanofibers possess a negatively charged surface of - 14.7 mV.					
36593253	1	76	theme	artificial	244:253	arg1	skin					255:258	artificial skin	244:258	artificial skin	244:258	The bacterial nanocellulose has been used in a wide range of biomedical applications including carriers for drug delivery, blood vessels, artificial skin and wound dressing.					
36593253	9	77	theme	crystallinity	1016:1028	arg1	value					1037:1041	crystallinity degree value	1016:1041	crystallinity degree value of 79.58%	1016:1051	X-ray diffraction show that the obtained bacterial nanocellulose has crystallinity degree value of 79.58%.					
36593253	13	78	theme	maximum	1400:1406	arg1	production was					1432:1445	maximum bacterial nanocellulose production was	1400:1445	maximum bacterial nanocellulose production was	1400:1445	Using the desirability function, the optimum conditions for maximum bacterial nanocellulose production was determined theoretically and verified experimentally.					
36593253	10	79	theme	characteristic	1080:1093	arg1	bands					1095:1099	the characteristic bands	1076:1099	the characteristic bands of the cellulose crystalline structure	1076:1138	FTIR spectra revealed the characteristic bands of the cellulose crystalline structure.					
36593253	15	80	theme	pH	1743:1744	arg1	level					1746:1750	an initial pH level	1732:1750	an initial pH level of 5	1732:1755	strain SEE-3 was obtained using medium volume; 100 mL/250 mL conical flask, inoculum size; 5%, v/v, citric acid; 1.5 g/L, yeast extract; 5 g/L, temperature; 37 °C, Na2HPO4; 3 g/L, an initial pH level of 5, Cantaloupe juice concentration of 81.27 percent and peptone 11.22 g/L.					
36593253	2	81	theme	produce bacterial	378:394	arg1	BNC					411:413	BNC	411:413	BNC	411:413	The total of ten morphologically different bacterial strains were screened for their potential to produce bacterial nanocellulose (BNC).					
36593253	2	81	theme	produce bacterial	378:394	arg1	nanocellulose					396:408	produce bacterial nanocellulose	378:408	produce bacterial nanocellulose (BNC)	378:414	The total of ten morphologically different bacterial strains were screened for their potential to produce bacterial nanocellulose (BNC).					
36593253	6	82	theme	charged	689:695	arg1	of - 14.7 mV					705:716	a negatively charged surface of - 14.7 mV	676:716	a negatively charged surface of - 14.7 mV	676:716	The cellulose nanofibers possess a negatively charged surface of - 14.7 mV.					
36593253	8	83	theme	TEM	852:854	arg1	images					856:861	The TEM images	848:861	The TEM images	848:861	The TEM images show needle-shaped particles with diameters of 30‒40 nm and lengths of 560‒1400 nm.					
35661668	3	0	theme	aqueous	659:665	arg1	electrospun					684:694	electrospun	684:694	electrospun	684:694	Once obtained, the aqueous suspensions were electrospun to prepare non-woven membranes, showing a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm), which was found to be influenced by the size (between 20 and 35 nm) of the embedded metal nanoparticles.					
35661668	3	0	theme	aqueous	659:665	arg1	suspensions					667:677	the aqueous suspensions	655:677	the aqueous suspensions	655:677	Once obtained, the aqueous suspensions were electrospun to prepare non-woven membranes, showing a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm), which was found to be influenced by the size (between 20 and 35 nm) of the embedded metal nanoparticles.					
35661668	3	1	theme	metal	903:907	arg1	nanoparticles					909:921	the embedded metal nanoparticles	890:921	the embedded metal nanoparticles	890:921	Once obtained, the aqueous suspensions were electrospun to prepare non-woven membranes, showing a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm), which was found to be influenced by the size (between 20 and 35 nm) of the embedded metal nanoparticles.					
35661668	5	2	theme	nanomolar	1225:1233	arg1	regime					1235:1240	the nanomolar regime	1221:1240	the nanomolar regime	1221:1240	The results showed that the antimicrobial response of the investigated samples occurred within a day of incubation and can be observed for AgNPs content in the polysaccharide fibers far below the nanomolar regime.					
35661668	3	3	theme	homogeneous	738:748	arg1	texture					765:771	a homogeneous nanostructured texture	736:771	a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm)	736:816	Once obtained, the aqueous suspensions were electrospun to prepare non-woven membranes, showing a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm), which was found to be influenced by the size (between 20 and 35 nm) of the embedded metal nanoparticles.					
35661668	1	4	theme	common	192:197	arg1	materials					213:221	common antimicrobial materials	192:221	common antimicrobial materials	192:221	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	3	5	with	texture	765:771	arg1	diameter					785:792	fiber diameter	779:792	fiber diameter between 100 and 150 nm	779:815	Once obtained, the aqueous suspensions were electrospun to prepare non-woven membranes, showing a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm), which was found to be influenced by the size (between 20 and 35 nm) of the embedded metal nanoparticles.					
35661668	3	6	theme	nanostructured	750:763	arg1	texture					765:771	a homogeneous nanostructured texture	736:771	a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm)	736:816	Once obtained, the aqueous suspensions were electrospun to prepare non-woven membranes, showing a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm), which was found to be influenced by the size (between 20 and 35 nm) of the embedded metal nanoparticles.					
35661668	5	7	from	content	1174:1180	arg1	fibers					1204:1209	the polysaccharide fibers	1185:1209	the polysaccharide fibers far below the nanomolar regime	1185:1240	The results showed that the antimicrobial response of the investigated samples occurred within a day of incubation and can be observed for AgNPs content in the polysaccharide fibers far below the nanomolar regime.					
35661668	4	8	theme	mats	968:971	arg1	potential					937:945	The biocidal potential	924:945	The biocidal potential of the nanocomposite mats	924:971	The biocidal potential of the nanocomposite mats was preliminarily tested against Gram-negative E. coli.					
35661668	1	9	theme	biocompatibility	311:326	arg1	safety					346:351	the biocompatibility and environmental safety	307:351	the biocompatibility and environmental safety of the naturally occurring polymeric component	307:398	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	5	10	theme	incubation	1133:1142	arg1	day					1126:1128	a day	1124:1128	a day of incubation	1124:1142	The results showed that the antimicrobial response of the investigated samples occurred within a day of incubation and can be observed for AgNPs content in the polysaccharide fibers far below the nanomolar regime.					
35661668	1	11	theme	Polysaccharide-based	84:103	arg1	composites					105:114	Polysaccharide-based composites	84:114	Polysaccharide-based composites embedding silver nanoparticles (AgNPs)	84:153	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	3	12	dep	obtained	645:652	arg1	Once					640:643	Once	640:643	Once	640:643	Once obtained, the aqueous suspensions were electrospun to prepare non-woven membranes, showing a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm), which was found to be influenced by the size (between 20 and 35 nm) of the embedded metal nanoparticles.					
35661668	1	13	theme	antimicrobial	199:211	arg1	materials					213:221	common antimicrobial materials	192:221	common antimicrobial materials	192:221	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	2	14	theme	chemical	552:559	arg1	approach					561:568	a wet chemical approach	546:568	a wet chemical approach carried out at different concentrations of the silver salt precursor	546:637	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	3	15	theme	nanoparticles	909:921	arg1	size					859:862	the size	855:862	the size (between 20 and 35 nm) of the embedded metal nanoparticles	855:921	Once obtained, the aqueous suspensions were electrospun to prepare non-woven membranes, showing a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm), which was found to be influenced by the size (between 20 and 35 nm) of the embedded metal nanoparticles.					
35661668	3	16	theme	fiber	779:783	arg1	diameter					785:792	fiber diameter	779:792	fiber diameter between 100 and 150 nm	779:815	Once obtained, the aqueous suspensions were electrospun to prepare non-woven membranes, showing a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm), which was found to be influenced by the size (between 20 and 35 nm) of the embedded metal nanoparticles.					
35661668	5	17	theme	polysaccharide	1189:1202	arg1	fibers					1204:1209	the polysaccharide fibers	1185:1209	the polysaccharide fibers far below the nanomolar regime	1185:1240	The results showed that the antimicrobial response of the investigated samples occurred within a day of incubation and can be observed for AgNPs content in the polysaccharide fibers far below the nanomolar regime.					
35661668	2	18	theme	alginate	437:444	arg1	chains					446:451	alginate chains	437:451	alginate chains (Alg@AgNPs)	437:463	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	2	18	theme	alginate	437:444	arg1	AgNPs					458:462	Alg@AgNPs	454:462	Alg@AgNPs	454:462	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	0	19	theme	Electrospun	0:10	arg1	alginate					12:19	Electrospun alginate	0:19	Electrospun alginate	0:19	Electrospun alginate mats embedding silver nanoparticles with bioactive properties.					
35661668	4	20	theme	nanocomposite	954:966	arg1	mats					968:971	the nanocomposite mats	950:971	the nanocomposite mats	950:971	The biocidal potential of the nanocomposite mats was preliminarily tested against Gram-negative E. coli.					
35661668	1	21	theme	silver	126:131	arg1	AgNPs					148:152	AgNPs	148:152	AgNPs	148:152	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	1	21	theme	silver	126:131	arg1	nanoparticles					133:145	silver nanoparticles	126:145	silver nanoparticles (AgNPs)	126:153	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	0	22	theme	embedding	26:34	arg1	nanoparticles					43:55	embedding silver nanoparticles	26:55	embedding silver nanoparticles with bioactive properties	26:81	Electrospun alginate mats embedding silver nanoparticles with bioactive properties.					
35661668	1	23	theme	environmental	332:344	arg1	safety					346:351	the biocompatibility and environmental safety	307:351	the biocompatibility and environmental safety of the naturally occurring polymeric component	307:398	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	1	24	theme	effective	238:246	arg1	properties					273:282	the effective, broad-spectrum biocidal properties	234:282	the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component	234:398	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	2	25	theme	polysaccharide	514:527	arg1	solution					529:536	the polysaccharide solution	510:536	the polysaccharide solution	510:536	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	2	26	theme	different	585:593	arg1	concentrations					595:608	different concentrations	585:608	different concentrations of the silver salt precursor	585:637	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	3	27	theme	non-woven	707:715	arg1	membranes					717:725	non-woven membranes	707:725	non-woven membranes	707:725	Once obtained, the aqueous suspensions were electrospun to prepare non-woven membranes, showing a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm), which was found to be influenced by the size (between 20 and 35 nm) of the embedded metal nanoparticles.					
35661668	2	28	theme	@	457:457	arg1	chains					446:451	alginate chains	437:451	alginate chains (Alg@AgNPs)	437:463	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	2	28	theme	@	457:457	arg1	AgNPs					458:462	Alg@AgNPs	454:462	Alg@AgNPs	454:462	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	0	29	theme	silver	36:41	arg1	nanoparticles					43:55	embedding silver nanoparticles	26:55	embedding silver nanoparticles with bioactive properties	26:81	Electrospun alginate mats embedding silver nanoparticles with bioactive properties.					
35661668	2	30	theme	Alg	454:456	arg1	chains					446:451	alginate chains	437:451	alginate chains (Alg@AgNPs)	437:463	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	2	30	theme	Alg	454:456	arg1	AgNPs					458:462	Alg@AgNPs	454:462	Alg@AgNPs	454:462	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	0	31	theme	bioactive	62:70	arg1	properties					72:81	bioactive properties	62:81	bioactive properties	62:81	Electrospun alginate mats embedding silver nanoparticles with bioactive properties.					
35661668	2	32	theme	precursor	629:637	arg1	concentrations					595:608	different concentrations	585:608	different concentrations of the silver salt precursor	585:637	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	5	33	theme	antimicrobial	1057:1069	arg1	response					1071:1078	the antimicrobial response	1053:1078	the antimicrobial response of the investigated samples	1053:1106	The results showed that the antimicrobial response of the investigated samples occurred within a day of incubation and can be observed for AgNPs content in the polysaccharide fibers far below the nanomolar regime.					
35661668	4	34	theme	biocidal	928:935	arg1	potential					937:945	The biocidal potential	924:945	The biocidal potential of the nanocomposite mats	924:971	The biocidal potential of the nanocomposite mats was preliminarily tested against Gram-negative E. coli.					
35661668	5	35	theme	AgNPs	1168:1172	arg1	content					1174:1180	AgNPs content	1168:1180	AgNPs content in the polysaccharide fibers far below the nanomolar regime	1168:1240	The results showed that the antimicrobial response of the investigated samples occurred within a day of incubation and can be observed for AgNPs content in the polysaccharide fibers far below the nanomolar regime.					
35661668	1	36	theme	occurring	370:378	arg1	component					390:398	the naturally occurring polymeric component	356:398	the naturally occurring polymeric component	356:398	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	2	37	theme	salt	624:627	arg1	precursor					629:637	the silver salt precursor	613:637	the silver salt precursor	613:637	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	1	38	theme	polymeric	380:388	arg1	component					390:398	the naturally occurring polymeric component	356:398	the naturally occurring polymeric component	356:398	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	2	39	theme	silver	617:622	arg1	precursor					629:637	the silver salt precursor	613:637	the silver salt precursor	613:637	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	1	40	theme	biocidal	264:271	arg1	properties					273:282	the effective, broad-spectrum biocidal properties	234:282	the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component	234:398	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	5	41	theme	investigated	1087:1098	arg1	samples					1100:1106	the investigated samples	1083:1106	the investigated samples	1083:1106	The results showed that the antimicrobial response of the investigated samples occurred within a day of incubation and can be observed for AgNPs content in the polysaccharide fibers far below the nanomolar regime.					
35661668	2	42	theme	wet	548:550	arg1	approach					561:568	a wet chemical approach	546:568	a wet chemical approach carried out at different concentrations of the silver salt precursor	546:637	In this work, AgNPs stabilized with alginate chains (Alg@AgNPs) were successfully synthesized in situ within the polysaccharide solution through a wet chemical approach carried out at different concentrations of the silver salt precursor.					
35661668	5	43	theme	samples	1100:1106	arg1	response					1071:1078	the antimicrobial response	1053:1078	the antimicrobial response of the investigated samples	1053:1106	The results showed that the antimicrobial response of the investigated samples occurred within a day of incubation and can be observed for AgNPs content in the polysaccharide fibers far below the nanomolar regime.					
35661668	1	44	theme	promising	167:175	arg1	alternative					177:187	a promising alternative	165:187	a promising alternative to common antimicrobial materials	165:221	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	1	45	theme	component	390:398	arg1	safety					346:351	the biocompatibility and environmental safety	307:351	the biocompatibility and environmental safety of the naturally occurring polymeric component	307:398	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	1	46	dep	effective	238:246	arg1	broad-spectrum					249:262	broad-spectrum	249:262	broad-spectrum	249:262	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
35661668	0	47	with	nanoparticles	43:55	arg1	properties					72:81	bioactive properties	62:81	bioactive properties	62:81	Electrospun alginate mats embedding silver nanoparticles with bioactive properties.					
35661668	3	48	theme	embedded	894:901	arg1	nanoparticles					909:921	the embedded metal nanoparticles	890:921	the embedded metal nanoparticles	890:921	Once obtained, the aqueous suspensions were electrospun to prepare non-woven membranes, showing a homogeneous nanostructured texture (with fiber diameter between 100 and 150 nm), which was found to be influenced by the size (between 20 and 35 nm) of the embedded metal nanoparticles.					
35661668	1	49	theme	AgNPs	287:291	arg1	properties					273:282	the effective, broad-spectrum biocidal properties	234:282	the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component	234:398	Polysaccharide-based composites embedding silver nanoparticles (AgNPs) represent a promising alternative to common antimicrobial materials because of the effective, broad-spectrum biocidal properties of AgNPs combined with the biocompatibility and environmental safety of the naturally occurring polymeric component.					
37217064	0	0	theme	Stability	93:101	arg1	evaluation					103:112	Stability evaluation	93:112	Co-encapsulation of probiotics with acylglycerols in gelatin-gum arabic complex coacervates: Stability evaluation under adverse conditions.	0:138	Co-encapsulation of probiotics with acylglycerols in gelatin-gum arabic complex coacervates: Stability evaluation under adverse conditions.					
37217064	3	1	theme	heat	709:712	arg1	treatment					714:722	heat treatment	709:722	heat treatment	709:722	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	3	1	theme	heat	709:712	arg1	stresses					684:691	environmental stresses	670:691	environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage)	670:762	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	8	2	theme	probiotics	1543:1552	arg1	strategy					1569:1576	a promising strategy	1557:1576	a promising strategy to resist adverse conditions	1557:1605	Therefore, co-microencapsulation of DAG oil and probiotics is a promising strategy to resist adverse conditions.					
37217064	8	2	theme	probiotics	1543:1552	arg1	co-microencapsulation					1506:1526	co-microencapsulation	1506:1526	co-microencapsulation of DAG oil and probiotics	1506:1552	Therefore, co-microencapsulation of DAG oil and probiotics is a promising strategy to resist adverse conditions.					
37217064	7	3	theme	DAG	1383:1385	arg1	presence					1371:1378	the presence	1367:1378	the presence of DAG	1367:1385	More importantly, GE-D-GA provided the best protection for probiotics under simulated gastrointestinal conditions, as the presence of DAG reduced cell damage during freeze-drying and the degree of contact between probiotics and digestive fluids.					
37217064	5	4	theme	high	1090:1093	arg1	rate					1117:1120	the high freeze-dried survival rate	1086:1120	the high freeze-dried survival rate (96.24 %) of GE-D-GA	1086:1141	These characteristics supported the high freeze-dried survival rate (96.24 %) of GE-D-GA.					
37217064	5	4	theme	high	1090:1093	arg1	%					1129:1129	96.24 %	1123:1129	96.24 %	1123:1129	These characteristics supported the high freeze-dried survival rate (96.24 %) of GE-D-GA.					
37217064	4	5	theme	protein	976:982	arg1	stability					963:971	the stability	959:971	the stability of protein and nucleic acid structure	959:1009	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	7	6	theme	digestive	1477:1485	arg1	fluids					1487:1492	digestive fluids	1477:1492	digestive fluids	1477:1492	More importantly, GE-D-GA provided the best protection for probiotics under simulated gastrointestinal conditions, as the presence of DAG reduced cell damage during freeze-drying and the degree of contact between probiotics and digestive fluids.					
37217064	2	7	theme	complex	356:362	arg1	coacervate					364:373	gelatin (GE)-gum arabic (GA) complex coacervate	327:373	gelatin (GE)-gum arabic (GA) complex coacervate	327:373	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	4	8	theme	cell	795:798	arg1	results					784:790	The results	780:790	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy	780:881	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	2	9	theme	gelatin	327:333	arg1	coacervate					364:373	gelatin (GE)-gum arabic (GA) complex coacervate	327:373	gelatin (GE)-gum arabic (GA) complex coacervate	327:373	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	4	10	theme	membrane	1044:1051	arg1	damage					1029:1034	the damage	1025:1034	the damage of cell membrane	1025:1051	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	4	11	theme	membrane	940:947	arg1	fluidity					923:930	the fluidity	919:930	the fluidity of cell membrane	919:947	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	3	12	theme	simulated	725:733	arg1	fluid					745:749	simulated digestive fluid	725:749	simulated digestive fluid	725:749	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	3	12	theme	simulated	725:733	arg1	stresses					684:691	environmental stresses	670:691	environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage)	670:762	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	7	13	theme	contact	1446:1452	arg1	freeze-drying					1414:1426	freeze-drying	1414:1426	freeze-drying	1414:1426	More importantly, GE-D-GA provided the best protection for probiotics under simulated gastrointestinal conditions, as the presence of DAG reduced cell damage during freeze-drying and the degree of contact between probiotics and digestive fluids.					
37217064	7	13	theme	contact	1446:1452	arg1	degree					1436:1441	the degree	1432:1441	the degree of contact between probiotics and digestive fluids	1432:1492	More importantly, GE-D-GA provided the best protection for probiotics under simulated gastrointestinal conditions, as the presence of DAG reduced cell damage during freeze-drying and the degree of contact between probiotics and digestive fluids.					
37217064	2	14	contain	containing	537:546	arg1	microcapsules					393:405	microcapsules	393:405	microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA)	393:517	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	14	contain	containing	537:546	arg2	probiotics					577:586	probiotics	577:586	probiotics (GE-D-GA)	577:596	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	14	contain	containing	537:546	arg2	oil					569:571	diacylglycerol (DAG) oil	548:571	diacylglycerol (DAG) oil	548:571	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	14	contain	containing	537:546	arg2	GE-D-GA					589:595	GE-D-GA	589:595	GE-D-GA	589:595	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	1	15	theme	acylglycerols	160:172	arg1	Co-encapsulation					140:155	Co-encapsulation	140:155	Co-encapsulation of acylglycerols and probiotics	140:187	Co-encapsulation of acylglycerols and probiotics may improve the resistance of probiotics to adverse conditions.					
37217064	3	16	theme	digestive	735:743	arg1	fluid					745:749	simulated digestive fluid	725:749	simulated digestive fluid	725:749	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	3	16	theme	digestive	735:743	arg1	stresses					684:691	environmental stresses	670:691	environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage)	670:762	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	2	17	contain	containing	457:466	arg1	microcapsules					443:455	microcapsules	443:455	microcapsules containing triacylglycerol (TAG) oil	443:492	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	17	contain	containing	457:466	arg2	oil					490:492	triacylglycerol (TAG) oil	468:492	triacylglycerol (TAG) oil	468:492	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	3	18	dep	stresses	684:691	arg1	storage					755:761	storage	755:761	storage	755:761	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	3	18	dep	stresses	684:691	arg1	fluid					745:749	simulated digestive fluid	725:749	simulated digestive fluid	725:749	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	3	18	dep	stresses	684:691	arg1	freeze-drying					694:706	freeze-drying	694:706	freeze-drying	694:706	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	3	18	dep	stresses	684:691	arg1	treatment					714:722	heat treatment	709:722	heat treatment	709:722	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	3	18	dep	stresses	684:691	arg1	stresses					684:691	environmental stresses	670:691	environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage)	670:762	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	7	19	theme	simulated	1325:1333	arg1	conditions					1352:1361	simulated gastrointestinal conditions	1325:1361	simulated gastrointestinal conditions	1325:1361	More importantly, GE-D-GA provided the best protection for probiotics under simulated gastrointestinal conditions, as the presence of DAG reduced cell damage during freeze-drying and the degree of contact between probiotics and digestive fluids.					
37217064	0	20	theme	adverse	120:126	arg1	conditions					128:137	adverse conditions	120:137	adverse conditions	120:137	Co-encapsulation of probiotics with acylglycerols in gelatin-gum arabic complex coacervates: Stability evaluation under adverse conditions.					
37217064	5	21	theme	survival	1108:1115	arg1	rate					1117:1120	the high freeze-dried survival rate	1086:1120	the high freeze-dried survival rate (96.24 %) of GE-D-GA	1086:1141	These characteristics supported the high freeze-dried survival rate (96.24 %) of GE-D-GA.					
37217064	5	21	theme	survival	1108:1115	arg1	%					1129:1129	96.24 %	1123:1129	96.24 %	1123:1129	These characteristics supported the high freeze-dried survival rate (96.24 %) of GE-D-GA.					
37217064	4	22	theme	Fourier	836:842	arg1	results					784:790	The results	780:790	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy	780:881	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	4	23	dep	infrared	854:861	arg1	FTIR					864:867	FTIR	864:867	FTIR	864:867	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	3	24	from	effects	614:620	arg1	cells					658:662	probiotic cells	648:662	probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage)	648:762	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	4	25	dep	cell	795:798	arg1	composition					820:830	membrane fatty acid composition	800:830	membrane fatty acid composition	800:830	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	0	26	from	acylglycerols	36:48	arg1	coacervates					80:90	gelatin-gum arabic complex coacervates	53:90	gelatin-gum arabic complex coacervates	53:90	Co-encapsulation of probiotics with acylglycerols in gelatin-gum arabic complex coacervates: Stability evaluation under adverse conditions.					
37217064	4	27	dep	Fourier	836:842	arg1	transform					844:852	transform	844:852	transform infrared (FTIR) spectroscopy	844:881	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	2	28	theme	-gum	339:342	arg1	coacervate					364:373	gelatin (GE)-gum arabic (GA) complex coacervate	327:373	gelatin (GE)-gum arabic (GA) complex coacervate	327:373	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	4	29	dep	protein	976:982	arg1	structure					1001:1009	structure	1001:1009	structure	1001:1009	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	4	30	theme	cell	935:938	arg1	membrane					940:947	cell membrane	935:947	cell membrane	935:947	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	8	31	theme	promising	1559:1567	arg1	strategy					1569:1576	a promising strategy	1557:1576	a promising strategy to resist adverse conditions	1557:1605	Therefore, co-microencapsulation of DAG oil and probiotics is a promising strategy to resist adverse conditions.					
37217064	8	31	theme	promising	1559:1567	arg1	co-microencapsulation					1506:1526	co-microencapsulation	1506:1526	co-microencapsulation of DAG oil and probiotics	1506:1552	Therefore, co-microencapsulation of DAG oil and probiotics is a promising strategy to resist adverse conditions.					
37217064	4	32	theme	infrared	854:861	arg1	spectroscopy					870:881	infrared (FTIR) spectroscopy	854:881	infrared (FTIR) spectroscopy	854:881	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	3	33	theme	environmental	670:682	arg1	storage					755:761	storage	755:761	storage	755:761	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	3	33	theme	environmental	670:682	arg1	fluid					745:749	simulated digestive fluid	725:749	simulated digestive fluid	725:749	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	3	33	theme	environmental	670:682	arg1	freeze-drying					694:706	freeze-drying	694:706	freeze-drying	694:706	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	3	33	theme	environmental	670:682	arg1	treatment					714:722	heat treatment	709:722	heat treatment	709:722	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	3	33	theme	environmental	670:682	arg1	stresses					684:691	environmental stresses	670:691	environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage)	670:762	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	1	34	theme	probiotics	219:228	arg1	resistance					205:214	the resistance	201:214	the resistance of probiotics to adverse conditions	201:250	Co-encapsulation of acylglycerols and probiotics may improve the resistance of probiotics to adverse conditions.					
37217064	4	35	theme	membrane	800:807	arg1	composition					820:830	membrane fatty acid composition	800:830	membrane fatty acid composition	800:830	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	2	36	theme	DAG	564:566	arg1	oil					569:571	diacylglycerol (DAG) oil	548:571	diacylglycerol (DAG) oil	548:571	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	37	theme	GE	336:337	arg1	coacervate					364:373	gelatin (GE)-gum arabic (GA) complex coacervate	327:373	gelatin (GE)-gum arabic (GA) complex coacervate	327:373	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	6	38	theme	cell	1223:1226	arg1	viability					1228:1236	the best cell viability retention	1214:1246	the best cell viability retention	1214:1246	Furthermore, regardless of thermotolerance or storage, GE-D-GA showed the best cell viability retention.					
37217064	0	39	theme	probiotics	20:29	arg1	Co-encapsulation					0:15	Co-encapsulation	0:15	Co-encapsulation of probiotics with acylglycerols in gelatin-gum arabic complex coacervates: Stability evaluation under adverse conditions.	0:138	Co-encapsulation of probiotics with acylglycerols in gelatin-gum arabic complex coacervates: Stability evaluation under adverse conditions.					
37217064	8	40	theme	oil	1535:1537	arg1	strategy					1569:1576	a promising strategy	1557:1576	a promising strategy to resist adverse conditions	1557:1605	Therefore, co-microencapsulation of DAG oil and probiotics is a promising strategy to resist adverse conditions.					
37217064	8	40	theme	oil	1535:1537	arg1	co-microencapsulation					1506:1526	co-microencapsulation	1506:1526	co-microencapsulation of DAG oil and probiotics	1506:1552	Therefore, co-microencapsulation of DAG oil and probiotics is a promising strategy to resist adverse conditions.					
37217064	1	41	theme	adverse	233:239	arg1	conditions					241:250	adverse conditions	233:250	adverse conditions	233:250	Co-encapsulation of acylglycerols and probiotics may improve the resistance of probiotics to adverse conditions.					
37217064	4	42	theme	acid	996:999	arg1	stability					963:971	the stability	959:971	the stability of protein and nucleic acid structure	959:1009	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	2	43	theme	diacylglycerol	548:561	arg1	oil					569:571	diacylglycerol (DAG) oil	548:571	diacylglycerol (DAG) oil	548:571	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	3	44	theme	probiotic	648:656	arg1	cells					658:662	probiotic cells	648:662	probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage)	648:762	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	4	45	theme	acid	815:818	arg1	composition					820:830	membrane fatty acid composition	800:830	membrane fatty acid composition	800:830	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	4	46	theme	nucleic	988:994	arg1	acid					996:999	nucleic acid	988:999	nucleic acid	988:999	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	8	47	theme	adverse	1588:1594	arg1	conditions					1596:1605	adverse conditions	1588:1605	adverse conditions	1588:1605	Therefore, co-microencapsulation of DAG oil and probiotics is a promising strategy to resist adverse conditions.					
37217064	4	48	theme	fatty	809:813	arg1	composition					820:830	membrane fatty acid composition	800:830	membrane fatty acid composition	800:830	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
37217064	2	49	theme	only	418:421	arg1	probiotics					423:432	only probiotics	418:432	only probiotics (GE-GA)	418:440	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	49	theme	only	418:421	arg1	GE-GA					435:439	GE-GA	435:439	GE-GA	435:439	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	0	50	theme	arabic	65:70	arg1	coacervates					80:90	gelatin-gum arabic complex coacervates	53:90	gelatin-gum arabic complex coacervates	53:90	Co-encapsulation of probiotics with acylglycerols in gelatin-gum arabic complex coacervates: Stability evaluation under adverse conditions.					
37217064	5	51	theme	GE-D-GA	1135:1141	arg1	rate					1117:1120	the high freeze-dried survival rate	1086:1120	the high freeze-dried survival rate (96.24 %) of GE-D-GA	1086:1141	These characteristics supported the high freeze-dried survival rate (96.24 %) of GE-D-GA.					
37217064	5	51	theme	GE-D-GA	1135:1141	arg1	%					1129:1129	96.24 %	1123:1129	96.24 %	1123:1129	These characteristics supported the high freeze-dried survival rate (96.24 %) of GE-D-GA.					
37217064	2	52	contain	containing	407:416	arg1	microcapsules					393:405	microcapsules	393:405	microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA)	393:517	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	52	contain	containing	407:416	arg2	GE-GA					435:439	GE-GA	435:439	GE-GA	435:439	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	52	contain	containing	407:416	arg2	probiotics					498:507	probiotics	498:507	probiotics (GE-T-GA)	498:517	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	52	contain	containing	407:416	arg2	GE-T-GA					510:516	GE-T-GA	510:516	GE-T-GA	510:516	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	52	contain	containing	407:416	arg2	probiotics					423:432	only probiotics	418:432	only probiotics (GE-GA)	418:440	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	52	contain	containing	407:416	arg2	microcapsules					443:455	microcapsules	443:455	microcapsules containing triacylglycerol (TAG) oil	443:492	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	0	53	with	probiotics	20:29	arg1	acylglycerols					36:48	acylglycerols	36:48	acylglycerols in gelatin-gum arabic complex coacervates	36:90	Co-encapsulation of probiotics with acylglycerols in gelatin-gum arabic complex coacervates: Stability evaluation under adverse conditions.					
37217064	0	54	theme	gelatin-gum	53:63	arg1	coacervates					80:90	gelatin-gum arabic complex coacervates	53:90	gelatin-gum arabic complex coacervates	53:90	Co-encapsulation of probiotics with acylglycerols in gelatin-gum arabic complex coacervates: Stability evaluation under adverse conditions.					
37217064	2	55	dep	constructed	309:319	arg1	microcapsules					393:405	microcapsules	393:405	microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA)	393:517	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	55	dep	constructed	309:319	arg1	microcapsules					523:535	microcapsules	523:535	microcapsules	523:535	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	56	theme	triacylglycerol	468:482	arg1	oil					490:492	triacylglycerol (TAG) oil	468:492	triacylglycerol (TAG) oil	468:492	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	57	theme	GA	352:353	arg1	coacervate					364:373	gelatin (GE)-gum arabic (GA) complex coacervate	327:373	gelatin (GE)-gum arabic (GA) complex coacervate	327:373	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	3	58	theme	protective	603:612	arg1	effects					614:620	The protective effects	599:620	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage)	599:762	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	5	59	theme	freeze-dried	1095:1106	arg1	rate					1117:1120	the high freeze-dried survival rate	1086:1120	the high freeze-dried survival rate (96.24 %) of GE-D-GA	1086:1141	These characteristics supported the high freeze-dried survival rate (96.24 %) of GE-D-GA.					
37217064	5	59	theme	freeze-dried	1095:1106	arg1	%					1129:1129	96.24 %	1123:1129	96.24 %	1123:1129	These characteristics supported the high freeze-dried survival rate (96.24 %) of GE-D-GA.					
37217064	6	60	theme	best	1218:1221	arg1	viability					1228:1236	the best cell viability retention	1214:1246	the best cell viability retention	1214:1246	Furthermore, regardless of thermotolerance or storage, GE-D-GA showed the best cell viability retention.					
37217064	3	61	theme	microcapsules	631:643	arg1	effects					614:620	The protective effects	599:620	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage)	599:762	The protective effects of three microcapsules on probiotic cells under environmental stresses (freeze-drying, heat treatment, simulated digestive fluid and storage) were evaluated.					
37217064	7	62	theme	cell	1395:1398	arg1	damage					1400:1405	cell damage	1395:1405	cell damage	1395:1405	More importantly, GE-D-GA provided the best protection for probiotics under simulated gastrointestinal conditions, as the presence of DAG reduced cell damage during freeze-drying and the degree of contact between probiotics and digestive fluids.					
37217064	6	63	dep	viability	1228:1236	arg1	retention					1238:1246	retention	1238:1246	the best cell viability retention	1214:1246	Furthermore, regardless of thermotolerance or storage, GE-D-GA showed the best cell viability retention.					
37217064	2	64	theme	microcapsule	284:295	arg1	models					297:302	three probiotic microcapsule models	268:302	three probiotic microcapsule models	268:302	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	65	theme	arabic	344:349	arg1	coacervate					364:373	gelatin (GE)-gum arabic (GA) complex coacervate	327:373	gelatin (GE)-gum arabic (GA) complex coacervate	327:373	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	8	66	theme	DAG	1531:1533	arg1	oil					1535:1537	DAG oil	1531:1537	DAG oil	1531:1537	Therefore, co-microencapsulation of DAG oil and probiotics is a promising strategy to resist adverse conditions.					
37217064	7	67	theme	best	1288:1291	arg1	protection					1293:1302	the best protection	1284:1302	the best protection for probiotics	1284:1317	More importantly, GE-D-GA provided the best protection for probiotics under simulated gastrointestinal conditions, as the presence of DAG reduced cell damage during freeze-drying and the degree of contact between probiotics and digestive fluids.					
37217064	2	68	theme	probiotic	274:282	arg1	models					297:302	three probiotic microcapsule models	268:302	three probiotic microcapsule models	268:302	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	2	69	theme	wall	378:381	arg1	material					383:390	wall material	378:390	wall material	378:390	In this study, three probiotic microcapsule models were constructed using gelatin (GE)-gum arabic (GA) complex coacervate as wall material: microcapsules containing only probiotics (GE-GA), microcapsules containing triacylglycerol (TAG) oil and probiotics (GE-T-GA) and microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA).					
37217064	7	70	theme	gastrointestinal	1335:1350	arg1	conditions					1352:1361	simulated gastrointestinal conditions	1325:1361	simulated gastrointestinal conditions	1325:1361	More importantly, GE-D-GA provided the best protection for probiotics under simulated gastrointestinal conditions, as the presence of DAG reduced cell damage during freeze-drying and the degree of contact between probiotics and digestive fluids.					
37217064	0	71	theme	complex	72:78	arg1	coacervates					80:90	gelatin-gum arabic complex coacervates	53:90	gelatin-gum arabic complex coacervates	53:90	Co-encapsulation of probiotics with acylglycerols in gelatin-gum arabic complex coacervates: Stability evaluation under adverse conditions.					
37217064	0	72	dep	Co-encapsulation	0:15	arg1	evaluation					103:112	Stability evaluation	93:112	Co-encapsulation of probiotics with acylglycerols in gelatin-gum arabic complex coacervates: Stability evaluation under adverse conditions.	0:138	Co-encapsulation of probiotics with acylglycerols in gelatin-gum arabic complex coacervates: Stability evaluation under adverse conditions.					
37217064	1	73	theme	probiotics	178:187	arg1	Co-encapsulation					140:155	Co-encapsulation	140:155	Co-encapsulation of acylglycerols and probiotics	140:187	Co-encapsulation of acylglycerols and probiotics may improve the resistance of probiotics to adverse conditions.					
37217064	4	74	theme	cell	1039:1042	arg1	membrane					1044:1051	cell membrane	1039:1051	cell membrane	1039:1051	The results of cell membrane fatty acid composition and Fourier transform infrared (FTIR) spectroscopy revealed that GE-D-GA could improve the fluidity of cell membrane, maintain the stability of protein and nucleic acid structure, and decrease the damage of cell membrane.					
36725979	7	0	theme	weight	1247:1252	arg1	gain					1254:1257	weight gain	1247:1257	weight gain	1247:1257	Increased sugar consumption also primes mice for increased caloric intake and weight gain when given access to a HFD.					
36725979	1	1	theme	sugar	371:375	arg1	content					377:383	the increased sugar content	357:383	the increased sugar content in processed foods	357:402	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	9	2	dep	palatable	1490:1498	arg1	HFD					1501:1503	HFD	1501:1503	HFD	1501:1503	This may contribute to obesity in mice and humans with access to more palatable (HFD) diets.					
36725979	0	3	theme	neurons	79:85	arg1	excitability					58:69	intrinsic and synaptic excitability	35:69	intrinsic and synaptic excitability of AgRP neurons	35:85	High sucrose consumption decouples intrinsic and synaptic excitability of AgRP neurons without altering body weight.					
36725979	1	4	theme	content	377:383	arg1	light					348:352	light	348:352	light of the increased sugar content in processed foods	348:402	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	6	5	theme	neuronal	1113:1120	arg1	excitability					1122:1133	AgRP neuronal excitability	1108:1133	AgRP neuronal excitability	1108:1133	When given sugar-sweetened water, mice show similar alterations to AgRP neuronal excitability as in high-fat diet obese models.					
36725979	1	6	theme	diet-induced	283:294	arg1	obesity					296:302	diet-induced obesity	283:302	diet-induced obesity	283:302	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	0	7	theme	body	104:107	arg1	weight					109:114	body weight	104:114	body weight	104:114	High sucrose consumption decouples intrinsic and synaptic excitability of AgRP neurons without altering body weight.					
36725979	8	8	theme	neurons	1377:1383	arg1	activity					1360:1367	activity	1360:1367	activity of AgRP neurons	1360:1383	CONCLUSIONS Our results show that elevated sucrose consumption increased activity of AgRP neurons and altered synaptic excitability.					
36725979	5	9	theme	subtle	982:987	arg1	differences					989:999	subtle differences	982:999	subtle differences in body composition and caloric intake	982:1038	RESULTS While mice given sugar-sweetened water do not gain significant weight, they do show subtle differences in body composition and caloric intake.					
36725979	5	10	from	differences	989:999	arg1	composition					1009:1019	body composition	1004:1019	body composition	1004:1019	RESULTS While mice given sugar-sweetened water do not gain significant weight, they do show subtle differences in body composition and caloric intake.					
36725979	5	10	from	differences	989:999	arg1	intake					1033:1038	caloric intake	1025:1038	caloric intake	1025:1038	RESULTS While mice given sugar-sweetened water do not gain significant weight, they do show subtle differences in body composition and caloric intake.					
36725979	7	11	theme	caloric	1228:1234	arg1	intake					1236:1241	increased caloric intake	1218:1241	increased caloric intake	1218:1241	Increased sugar consumption also primes mice for increased caloric intake and weight gain when given access to a HFD.					
36725979	1	12	theme	processed	388:396	arg1	foods					398:402	processed foods	388:402	processed foods	388:402	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	1	13	from	light	348:352	arg1	critical					256:263	critical	256:263	critical	256:263	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	1	13	from	light	348:352	arg1	foods					398:402	processed foods	388:402	processed foods	388:402	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	1	13	from	light	348:352	arg1	understanding					177:189	the understanding	173:189	the understanding of macronutrient influence on central nervous system function	173:251	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	8	14	theme	AgRP	1372:1375	arg1	neurons					1377:1383	AgRP neurons	1372:1383	AgRP neurons	1372:1383	CONCLUSIONS Our results show that elevated sucrose consumption increased activity of AgRP neurons and altered synaptic excitability.					
36725979	9	15	theme	palatable	1490:1498	arg1	diets					1506:1510	more palatable (HFD) diets	1485:1510	more palatable (HFD) diets	1485:1510	This may contribute to obesity in mice and humans with access to more palatable (HFD) diets.					
36725979	6	16	theme	high-fat	1141:1148	arg1	models					1161:1166	high-fat diet obese models	1141:1166	high-fat diet obese models	1141:1166	When given sugar-sweetened water, mice show similar alterations to AgRP neuronal excitability as in high-fat diet obese models.					
36725979	2	17	theme	sucrose	532:538	arg1	impact					522:527	the impact	518:527	the impact of sucrose on hypothalamic functioning	518:566	Previous research showed mixed effects of sucrose feeding on body weight gain but has yet to reveal insight into the impact of sucrose on hypothalamic functioning.					
36725979	3	18	theme	synaptic	730:737	arg1	plasticity					739:748	synaptic plasticity	730:748	synaptic plasticity	730:748	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	2	19	theme	feeding	455:461	arg1	effects					436:442	mixed effects	430:442	mixed effects of sucrose feeding on body weight gain	430:481	Previous research showed mixed effects of sucrose feeding on body weight gain but has yet to reveal insight into the impact of sucrose on hypothalamic functioning.					
36725979	4	20	theme	AgRP	805:808	arg1	neurons					810:816	hypothalamic AgRP neurons	792:816	hypothalamic AgRP neurons	792:816	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	6	21	theme	AgRP	1108:1111	arg1	excitability					1122:1133	AgRP neuronal excitability	1108:1133	AgRP neuronal excitability	1108:1133	When given sugar-sweetened water, mice show similar alterations to AgRP neuronal excitability as in high-fat diet obese models.					
36725979	4	22	dep	METHODS	751:757	arg1	METHODS					751:757	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake	751:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake	751:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	4	22	dep	METHODS	751:757	arg1	phenotyping					829:839	metabolic phenotyping	819:839	metabolic phenotyping	819:839	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	4	22	dep	METHODS	751:757	arg1	intake					850:855	food intake	845:855	food intake	845:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	4	22	dep	METHODS	751:757	arg1	electrophysiology					771:787	Patch-clamp electrophysiology	759:787	Patch-clamp electrophysiology of hypothalamic AgRP neurons	759:816	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	3	23	theme	neuronal	708:715	arg1	function					717:724	neuronal function	708:724	neuronal function	708:724	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	7	24	theme	sugar	1179:1183	arg1	consumption					1185:1195	Increased sugar consumption	1169:1195	Increased sugar consumption	1169:1195	Increased sugar consumption also primes mice for increased caloric intake and weight gain when given access to a HFD.					
36725979	4	25	theme	hypothalamic	792:803	arg1	neurons					810:816	hypothalamic AgRP neurons	792:816	hypothalamic AgRP neurons	792:816	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	2	26	theme	mixed	430:434	arg1	effects					436:442	mixed effects	430:442	mixed effects of sucrose feeding on body weight gain	430:481	Previous research showed mixed effects of sucrose feeding on body weight gain but has yet to reveal insight into the impact of sucrose on hypothalamic functioning.					
36725979	0	27	theme	sucrose	5:11	arg1	consumption					13:23	High sucrose consumption	0:23	High sucrose consumption	0:23	High sucrose consumption decouples intrinsic and synaptic excitability of AgRP neurons without altering body weight.					
36725979	3	28	dep	AgRP	703:706	arg1	function					717:724	neuronal function	708:724	neuronal function	708:724	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	9	29	from	obesity	1443:1449	arg1	mice					1454:1457	mice	1454:1457	mice	1454:1457	This may contribute to obesity in mice and humans with access to more palatable (HFD) diets.					
36725979	9	29	from	obesity	1443:1449	arg1	humans					1463:1468	humans	1463:1468	humans	1463:1468	This may contribute to obesity in mice and humans with access to more palatable (HFD) diets.					
36725979	1	30	theme	macronutrient	194:206	arg1	influence					208:216	macronutrient influence	194:216	macronutrient influence on central nervous system function	194:251	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	0	31	theme	High	0:3	arg1	consumption					13:23	High sucrose consumption	0:23	High sucrose consumption	0:23	High sucrose consumption decouples intrinsic and synaptic excitability of AgRP neurons without altering body weight.					
36725979	1	32	theme	potential	308:316	arg1	therapeutics					318:329	potential therapeutics	308:329	potential therapeutics	308:329	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	4	33	theme	C57BL/6J	875:882	arg1	mice					884:887	C57BL/6J mice	875:887	C57BL/6J mice	875:887	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	8	34	theme	synaptic	1397:1404	arg1	excitability					1406:1417	synaptic excitability	1397:1417	synaptic excitability	1397:1417	CONCLUSIONS Our results show that elevated sucrose consumption increased activity of AgRP neurons and altered synaptic excitability.					
36725979	1	35	theme	influence	208:216	arg1	critical					256:263	critical	256:263	critical	256:263	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	1	35	theme	influence	208:216	arg1	understanding					177:189	the understanding	173:189	the understanding of macronutrient influence on central nervous system function	173:251	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	2	36	theme	weight	471:476	arg1	gain					478:481	body weight gain	466:481	body weight gain	466:481	Previous research showed mixed effects of sucrose feeding on body weight gain but has yet to reveal insight into the impact of sucrose on hypothalamic functioning.					
36725979	8	37	theme	sucrose	1330:1336	arg1	consumption					1338:1348	elevated sucrose consumption	1321:1348	elevated sucrose consumption	1321:1348	CONCLUSIONS Our results show that elevated sucrose consumption increased activity of AgRP neurons and altered synaptic excitability.					
36725979	8	38	theme	elevated	1321:1328	arg1	consumption					1338:1348	elevated sucrose consumption	1321:1348	elevated sucrose consumption	1321:1348	CONCLUSIONS Our results show that elevated sucrose consumption increased activity of AgRP neurons and altered synaptic excitability.					
36725979	9	39	with	obesity	1443:1449	arg1	access					1475:1480	access	1475:1480	access to more palatable (HFD) diets	1475:1510	This may contribute to obesity in mice and humans with access to more palatable (HFD) diets.					
36725979	4	40	theme	Patch-clamp	759:769	arg1	METHODS					751:757	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake	751:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake	751:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	4	40	theme	Patch-clamp	759:769	arg1	electrophysiology					771:787	Patch-clamp electrophysiology	759:787	Patch-clamp electrophysiology of hypothalamic AgRP neurons	759:816	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	4	41	theme	food	845:848	arg1	METHODS					751:757	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake	751:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake	751:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	4	41	theme	food	845:848	arg1	intake					850:855	food intake	845:855	food intake	845:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	8	42	theme	CONCLUSIONS	1287:1297	arg1	results					1303:1309	CONCLUSIONS Our results	1287:1309	CONCLUSIONS Our results	1287:1309	CONCLUSIONS Our results show that elevated sucrose consumption increased activity of AgRP neurons and altered synaptic excitability.					
36725979	1	43	from	foods	398:402	arg1	light					348:352	light	348:352	light of the increased sugar content in processed foods	348:402	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	2	44	theme	Previous	405:412	arg1	research					414:421	Previous research	405:421	Previous research	405:421	Previous research showed mixed effects of sucrose feeding on body weight gain but has yet to reveal insight into the impact of sucrose on hypothalamic functioning.					
36725979	1	45	theme	central	221:227	arg1	system					237:242	central nervous system	221:242	central nervous system function	221:251	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	1	46	from	content	377:383	arg1	foods					398:402	processed foods	388:402	processed foods	388:402	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	3	47	theme	body	652:655	arg1	composition					657:667	body composition	652:667	body composition	652:667	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	7	48	theme	increased	1218:1226	arg1	intake					1236:1241	increased caloric intake	1218:1241	increased caloric intake	1218:1241	Increased sugar consumption also primes mice for increased caloric intake and weight gain when given access to a HFD.					
36725979	2	49	theme	sucrose	447:453	arg1	feeding					455:461	sucrose feeding	447:461	sucrose feeding	447:461	Previous research showed mixed effects of sucrose feeding on body weight gain but has yet to reveal insight into the impact of sucrose on hypothalamic functioning.					
36725979	3	50	theme	sucrose	607:613	arg1	feeding					615:621	liquid sucrose feeding	600:621	liquid sucrose feeding	600:621	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	6	51	theme	similar	1085:1091	arg1	alterations					1093:1103	similar alterations	1085:1103	similar alterations to AgRP neuronal excitability	1085:1133	When given sugar-sweetened water, mice show similar alterations to AgRP neuronal excitability as in high-fat diet obese models.					
36725979	1	52	theme	nervous	229:235	arg1	system					237:242	central nervous system	221:242	central nervous system function	221:251	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	0	53	theme	intrinsic	35:43	arg1	excitability					58:69	intrinsic and synaptic excitability	35:69	intrinsic and synaptic excitability of AgRP neurons	35:85	High sucrose consumption decouples intrinsic and synaptic excitability of AgRP neurons without altering body weight.					
36725979	2	54	from	impact	522:527	arg1	hypothalamic					543:554	hypothalamic	543:554	hypothalamic	543:554	Previous research showed mixed effects of sucrose feeding on body weight gain but has yet to reveal insight into the impact of sucrose on hypothalamic functioning.					
36725979	3	55	theme	feeding	615:621	arg1	impact					590:595	the impact	586:595	the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity	586:748	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	1	56	theme	system	237:242	arg1	function					244:251	central nervous system function	221:251	central nervous system function	221:251	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	1	57	from	critical	256:263	arg1	light					348:352	light	348:352	light of the increased sugar content in processed foods	348:402	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	6	58	theme	obese	1155:1159	arg1	models					1161:1166	high-fat diet obese models	1141:1166	high-fat diet obese models	1141:1166	When given sugar-sweetened water, mice show similar alterations to AgRP neuronal excitability as in high-fat diet obese models.					
36725979	0	59	theme	synaptic	49:56	arg1	excitability					58:69	intrinsic and synaptic excitability	35:69	intrinsic and synaptic excitability of AgRP neurons	35:85	High sucrose consumption decouples intrinsic and synaptic excitability of AgRP neurons without altering body weight.					
36725979	3	60	theme	hypothalamic	690:701	arg1	AgRP					703:706	hypothalamic AgRP neuronal function and synaptic plasticity	690:748	AgRP	703:706	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	3	61	theme	caloric	670:676	arg1	intake					678:683	caloric intake	670:683	caloric intake	670:683	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	3	62	theme	liquid	600:605	arg1	feeding					615:621	liquid sucrose feeding	600:621	liquid sucrose feeding	600:621	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	2	63	from	effects	436:442	arg1	gain					478:481	body weight gain	466:481	body weight gain	466:481	Previous research showed mixed effects of sucrose feeding on body weight gain but has yet to reveal insight into the impact of sucrose on hypothalamic functioning.					
36725979	3	64	theme	body	639:642	arg1	weight					644:649	body weight	639:649	body weight	639:649	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	4	65	theme	metabolic	819:827	arg1	METHODS					751:757	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake	751:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake	751:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	4	65	theme	metabolic	819:827	arg1	phenotyping					829:839	metabolic phenotyping	819:839	metabolic phenotyping	819:839	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	1	66	from	function	244:251	arg1	critical					256:263	critical	256:263	critical	256:263	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	1	66	from	function	244:251	arg1	understanding					177:189	the understanding	173:189	the understanding of macronutrient influence on central nervous system function	173:251	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	7	67	theme	Increased	1169:1177	arg1	consumption					1185:1195	Increased sugar consumption	1169:1195	Increased sugar consumption	1169:1195	Increased sugar consumption also primes mice for increased caloric intake and weight gain when given access to a HFD.					
36725979	5	68	theme	significant	949:959	arg1	weight					961:966	significant weight	949:966	significant weight	949:966	RESULTS While mice given sugar-sweetened water do not gain significant weight, they do show subtle differences in body composition and caloric intake.					
36725979	6	69	theme	sugar-sweetened	1052:1066	arg1	water					1068:1072	sugar-sweetened water	1052:1072	sugar-sweetened water	1052:1072	When given sugar-sweetened water, mice show similar alterations to AgRP neuronal excitability as in high-fat diet obese models.					
36725979	5	70	theme	body	1004:1007	arg1	composition					1009:1019	body composition	1004:1019	body composition	1004:1019	RESULTS While mice given sugar-sweetened water do not gain significant weight, they do show subtle differences in body composition and caloric intake.					
36725979	4	71	theme	neurons	810:816	arg1	METHODS					751:757	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake	751:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake	751:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	4	71	theme	neurons	810:816	arg1	phenotyping					829:839	metabolic phenotyping	819:839	metabolic phenotyping	819:839	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	4	71	theme	neurons	810:816	arg1	intake					850:855	food intake	845:855	food intake	845:855	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	4	71	theme	neurons	810:816	arg1	electrophysiology					771:787	Patch-clamp electrophysiology	759:787	Patch-clamp electrophysiology of hypothalamic AgRP neurons	759:816	METHODS Patch-clamp electrophysiology of hypothalamic AgRP neurons, metabolic phenotyping and food intake were performed on C57BL/6J mice.					
36725979	1	72	theme	obesity	145:151	arg1	continues					162:170	the obesity epidemic continues	141:170	the obesity epidemic continues	141:170	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	6	73	theme	diet	1150:1153	arg1	models					1161:1166	high-fat diet obese models	1141:1166	high-fat diet obese models	1141:1166	When given sugar-sweetened water, mice show similar alterations to AgRP neuronal excitability as in high-fat diet obese models.					
36725979	0	74	theme	AgRP	74:77	arg1	neurons					79:85	AgRP neurons	74:85	AgRP neurons	74:85	High sucrose consumption decouples intrinsic and synaptic excitability of AgRP neurons without altering body weight.					
36725979	5	75	theme	caloric	1025:1031	arg1	intake					1033:1038	caloric intake	1025:1038	caloric intake	1025:1038	RESULTS While mice given sugar-sweetened water do not gain significant weight, they do show subtle differences in body composition and caloric intake.					
36725979	1	76	from	influence	208:216	arg1	function					244:251	central nervous system function	221:251	central nervous system function	221:251	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	1	77	theme	epidemic	153:160	arg1	continues					162:170	the obesity epidemic continues	141:170	the obesity epidemic continues	141:170	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	3	78	from	impact	590:595	arg1	AgRP					703:706	hypothalamic AgRP neuronal function and synaptic plasticity	690:748	AgRP	703:706	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	3	78	from	impact	590:595	arg1	weight					644:649	body weight	639:649	body weight	639:649	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	3	78	from	impact	590:595	arg1	composition					657:667	body composition	652:667	body composition	652:667	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	3	78	from	impact	590:595	arg1	intake					678:683	caloric intake	670:683	caloric intake	670:683	Here, we explore the impact of liquid sucrose feeding for 12 weeks on body weight, body composition, caloric intake, and hypothalamic AgRP neuronal function and synaptic plasticity.					
36725979	1	79	theme	increased	361:369	arg1	content					377:383	the increased sugar content	357:383	the increased sugar content in processed foods	357:402	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	2	80	theme	body	466:469	arg1	gain					478:481	body weight gain	466:481	body weight gain	466:481	Previous research showed mixed effects of sucrose feeding on body weight gain but has yet to reveal insight into the impact of sucrose on hypothalamic functioning.					
36725979	1	81	from	understanding	177:189	arg1	function					244:251	central nervous system function	221:251	central nervous system function	221:251	BACKGROUND/OBJECTIVE As the obesity epidemic continues, the understanding of macronutrient influence on central nervous system function is critical for understanding diet-induced obesity and potential therapeutics, particularly in light of the increased sugar content in processed foods.					
36725979	5	82	theme	sugar-sweetened	915:929	arg1	water					931:935	sugar-sweetened water	915:935	sugar-sweetened water	915:935	RESULTS While mice given sugar-sweetened water do not gain significant weight, they do show subtle differences in body composition and caloric intake.					
36076415	5	0	theme	cold	1038:1041	arg1	conditions					1059:1068	cold and supermarket conditions	1038:1068	cold and supermarket conditions	1038:1068	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	1	1	theme	degradation	150:160	arg1	process					162:168	enzyme mediated degradation process	134:168	enzyme mediated degradation process determined by the action of cell wall modifying enzymes	134:224	Fruit softening is enzyme mediated degradation process determined by the action of cell wall modifying enzymes.					
36076415	2	2	from	fruit	420:424	arg1	modulation					325:334	modulation	325:334	modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	325:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	5	3	theme	2.0 mM	811:816	arg1	coating					818:824	SA 2.0 mM coating	808:824	SA 2.0 mM coating	808:824	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	5	4	theme	SA	808:809	arg1	coating					818:824	SA 2.0 mM coating	808:824	SA 2.0 mM coating	808:824	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	2	5	theme	supermarket	472:482	arg1	RH					506:507	20-22 °C and 80-85 % RH	485:507	RH	506:507	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	5	theme	supermarket	472:482	arg1	conditions					510:519	cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	439:519	conditions	510:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	4	6	theme	malondialdehyde	731:745	arg1	accumulation					753:764	the electrolyte leakage and malondialdehyde (MDA) accumulation	703:764	the electrolyte leakage and malondialdehyde (MDA) accumulation as compared to CH or SA alone	703:794	In addition, CH + SA prevented membrane damage by suppressing the electrolyte leakage and malondialdehyde (MDA) accumulation as compared to CH or SA alone.					
36076415	4	7	theme	electrolyte	707:717	arg1	leakage					719:725	electrolyte leakage	707:725	electrolyte leakage	707:725	In addition, CH + SA prevented membrane damage by suppressing the electrolyte leakage and malondialdehyde (MDA) accumulation as compared to CH or SA alone.					
36076415	5	8	theme	pectin	901:906	arg1	PME					924:926	PME	924:926	PME	924:926	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	5	8	theme	pectin	901:906	arg1	methylesterase					908:921	pectin methylesterase	901:921	pectin methylesterase (PME)	901:927	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	5	9	theme	15 days	1012:1018	arg1	period					1028:1033	15 days storage period	1012:1033	15 days storage period	1012:1033	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	2	10	theme	chitosan	275:282	arg1	coatings					313:320	chitosan (CH) and salicylic acid (SA) coatings	275:320	chitosan (CH) and salicylic acid (SA) coatings	275:320	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	5	11	theme	2.0	800:802	arg1	%					804:804	%	804:804	%	804:804	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	2	12	theme	Punjab	366:371	arg1	pear					380:383	Punjab Beauty pear	366:383	Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	366:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	12	theme	Punjab	366:371	arg1	Pyrus					386:390	Pyrus	386:390	Pyrus	386:390	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	0	13	theme	supermarket	94:104	arg1	storage					106:112	supermarket storage	94:112	supermarket storage	94:112	Composite coating of chitosan with salicylic acid retards pear fruit softening under cold and supermarket storage.					
36076415	5	14	theme	storage	1020:1026	arg1	period					1028:1033	15 days storage period	1012:1033	15 days storage period	1012:1033	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	2	15	from	modulation	325:334	arg1	fruit					420:424	Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit	366:424	Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	366:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	4	16	theme	leakage	719:725	arg1	accumulation					753:764	the electrolyte leakage and malondialdehyde (MDA) accumulation	703:764	the electrolyte leakage and malondialdehyde (MDA) accumulation as compared to CH or SA alone	703:794	In addition, CH + SA prevented membrane damage by suppressing the electrolyte leakage and malondialdehyde (MDA) accumulation as compared to CH or SA alone.					
36076415	2	17	theme	%	462:462	arg1	cold					439:442	cold	439:442	cold	439:442	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	17	theme	%	462:462	arg1	RH					464:465	0-1 °C and 90-95 % RH	445:465	0-1 °C and 90-95 % RH	445:465	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	18	theme	acid	303:306	arg1	coatings					313:320	chitosan (CH) and salicylic acid (SA) coatings	275:320	chitosan (CH) and salicylic acid (SA) coatings	275:320	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	19	theme	enzymes	355:361	arg1	modulation					325:334	modulation	325:334	modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	325:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	0	20	theme	Composite	0:8	arg1	coating					10:16	Composite coating	0:16	Composite coating of chitosan with salicylic acid	0:48	Composite coating of chitosan with salicylic acid retards pear fruit softening under cold and supermarket storage.					
36076415	3	21	dep	storage	625:631	arg1	67					610:611	67	610:611	67	610:611	Composite CH + SA coatings reduced mass loss and retained fruit firmness throughout the 67 and 20 days storage period.					
36076415	3	21	dep	storage	625:631	arg1	20 days					617:623	20 days	617:623	20 days	617:623	Composite CH + SA coatings reduced mass loss and retained fruit firmness throughout the 67 and 20 days storage period.					
36076415	5	22	theme	fruit	983:987	arg1	softening					989:997	fruit softening	983:997	fruit softening up to 60	983:1006	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	2	23	theme	salicylic	293:301	arg1	SA					309:310	SA	309:310	SA	309:310	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	23	theme	salicylic	293:301	arg1	acid					303:306	salicylic acid	293:306	salicylic acid (SA)	293:311	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	24	theme	softening	345:353	arg1	enzymes					355:361	fruit softening enzymes	339:361	fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	339:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	0	25	theme	chitosan	21:28	arg1	coating					10:16	Composite coating	0:16	Composite coating of chitosan with salicylic acid	0:48	Composite coating of chitosan with salicylic acid retards pear fruit softening under cold and supermarket storage.					
36076415	3	26	dep	period	633:638	arg1	storage					625:631	storage	625:631	storage	625:631	Composite CH + SA coatings reduced mass loss and retained fruit firmness throughout the 67 and 20 days storage period.					
36076415	2	27	theme	80-85	498:502	arg1	RH					506:507	20-22 °C and 80-85 % RH	485:507	RH	506:507	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	27	theme	80-85	498:502	arg1	conditions					510:519	cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	439:519	conditions	510:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	5	28	theme	enzymatic	870:878	arg1	cellulase					957:965	cellulase	957:965	cellulase associated with fruit softening up to 60	957:1006	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	5	28	theme	enzymatic	870:878	arg1	activities					880:889	enzymatic activities	870:889	enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60	870:1006	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	5	28	theme	enzymatic	870:878	arg1	methylesterase					908:921	pectin methylesterase	901:921	pectin methylesterase (PME)	901:927	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	5	28	theme	enzymatic	870:878	arg1	polygalacturonase					930:946	polygalacturonase	930:946	polygalacturonase (PG)	930:951	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	4	29	theme	membrane	672:679	arg1	damage					681:686	membrane damage	672:686	membrane damage	672:686	In addition, CH + SA prevented membrane damage by suppressing the electrolyte leakage and malondialdehyde (MDA) accumulation as compared to CH or SA alone.					
36076415	2	30	from	effects	264:270	arg1	modulation					325:334	modulation	325:334	modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	325:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	1	31	theme	cell	198:201	arg1	enzymes					218:224	cell wall modifying enzymes	198:224	cell wall modifying enzymes	198:224	Fruit softening is enzyme mediated degradation process determined by the action of cell wall modifying enzymes.					
36076415	0	32	theme	salicylic	35:43	arg1	acid					45:48	salicylic acid	35:48	salicylic acid	35:48	Composite coating of chitosan with salicylic acid retards pear fruit softening under cold and supermarket storage.					
36076415	2	33	theme	20-22 °C	485:492	arg1	RH					506:507	20-22 °C and 80-85 % RH	485:507	RH	506:507	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	33	theme	20-22 °C	485:492	arg1	conditions					510:519	cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	439:519	conditions	510:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	1	34	theme	wall	203:206	arg1	enzymes					218:224	cell wall modifying enzymes	198:224	cell wall modifying enzymes	198:224	Fruit softening is enzyme mediated degradation process determined by the action of cell wall modifying enzymes.					
36076415	3	35	theme	CH + SA	532:538	arg1	coatings					540:547	Composite CH + SA coatings	522:547	Composite CH + SA coatings	522:547	Composite CH + SA coatings reduced mass loss and retained fruit firmness throughout the 67 and 20 days storage period.					
36076415	5	36	theme	%	804:804	arg1	CH					797:798	CH	797:798	CH 2.0 %	797:804	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	2	37	theme	pear	380:383	arg1	fruit					420:424	Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit	366:424	Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	366:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	4	38	dep	accumulation	753:764	arg1	compared					769:776	compared	769:776	compared to CH or SA alone	769:794	In addition, CH + SA prevented membrane damage by suppressing the electrolyte leakage and malondialdehyde (MDA) accumulation as compared to CH or SA alone.					
36076415	2	39	theme	Present	227:233	arg1	study					235:239	Present study	227:239	Present study	227:239	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	40	from	enzymes	355:361	arg1	fruit					420:424	Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit	366:424	Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	366:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	1	41	theme	modifying	208:216	arg1	enzymes					218:224	cell wall modifying enzymes	198:224	cell wall modifying enzymes	198:224	Fruit softening is enzyme mediated degradation process determined by the action of cell wall modifying enzymes.					
36076415	3	42	theme	fruit	580:584	arg1	firmness					586:593	fruit firmness	580:593	fruit firmness	580:593	Composite CH + SA coatings reduced mass loss and retained fruit firmness throughout the 67 and 20 days storage period.					
36076415	2	43	dep	Pyrus	386:390	arg1	communis					410:417	Pyrus pyrifolia × Pyrus communis	386:417	Pyrus pyrifolia × Pyrus communis	386:417	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	44	theme	coatings	313:320	arg1	effects					264:270	the combined effects	251:270	the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	251:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	5	45	theme	supermarket	1047:1057	arg1	conditions					1059:1068	cold and supermarket conditions	1038:1068	cold and supermarket conditions	1038:1068	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	3	46	theme	Composite	522:530	arg1	coatings					540:547	Composite CH + SA coatings	522:547	Composite CH + SA coatings	522:547	Composite CH + SA coatings reduced mass loss and retained fruit firmness throughout the 67 and 20 days storage period.					
36076415	0	47	with	coating	10:16	arg1	acid					45:48	salicylic acid	35:48	salicylic acid	35:48	Composite coating of chitosan with salicylic acid retards pear fruit softening under cold and supermarket storage.					
36076415	1	48	theme	Fruit	115:119	arg1	softening					121:129	Fruit softening	115:129	Fruit softening	115:129	Fruit softening is enzyme mediated degradation process determined by the action of cell wall modifying enzymes.					
36076415	1	49	theme	enzyme	134:139	arg1	process					162:168	enzyme mediated degradation process	134:168	enzyme mediated degradation process determined by the action of cell wall modifying enzymes	134:224	Fruit softening is enzyme mediated degradation process determined by the action of cell wall modifying enzymes.					
36076415	1	50	theme	enzymes	218:224	arg1	action					188:193	the action	184:193	the action of cell wall modifying enzymes	184:224	Fruit softening is enzyme mediated degradation process determined by the action of cell wall modifying enzymes.					
36076415	2	51	theme	%	504:504	arg1	RH					506:507	20-22 °C and 80-85 % RH	485:507	RH	506:507	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	51	theme	%	504:504	arg1	conditions					510:519	cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	439:519	conditions	510:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	52	theme	combined	255:262	arg1	effects					264:270	the combined effects	251:270	the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	251:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	5	53	theme	cell	850:853	arg1	wall					855:858	the cell wall	846:858	the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60	846:1006	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	5	54	dep	60	1005:1006	arg1	to					1002:1003	to	1002:1003	to	1002:1003	CH 2.0 % + SA 2.0 mM coating efficiently delayed the cell wall degrading enzymatic activities including pectin methylesterase (PME), polygalacturonase (PG) and cellulase associated with fruit softening up to 60 and 15 days storage period in cold and supermarket conditions, respectively.					
36076415	0	55	theme	fruit	63:67	arg1	softening					69:77	pear fruit softening	58:77	pear fruit softening	58:77	Composite coating of chitosan with salicylic acid retards pear fruit softening under cold and supermarket storage.					
36076415	2	56	theme	Beauty	373:378	arg1	pear					380:383	Punjab Beauty pear	366:383	Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	366:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	56	theme	Beauty	373:378	arg1	Pyrus					386:390	Pyrus	386:390	Pyrus	386:390	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	2	57	theme	fruit	339:343	arg1	enzymes					355:361	fruit softening enzymes	339:361	fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions	339:519	Present study evaluated the combined effects of chitosan (CH) and salicylic acid (SA) coatings in modulation of fruit softening enzymes in Punjab Beauty pear (Pyrus pyrifolia × Pyrus communis) fruit stored under cold (0-1 °C and 90-95 % RH) and supermarket (20-22 °C and 80-85 % RH) conditions.					
36076415	0	58	theme	pear	58:61	arg1	softening					69:77	pear fruit softening	58:77	pear fruit softening	58:77	Composite coating of chitosan with salicylic acid retards pear fruit softening under cold and supermarket storage.					
36076415	3	59	theme	mass	557:560	arg1	loss					562:565	mass loss	557:565	mass loss	557:565	Composite CH + SA coatings reduced mass loss and retained fruit firmness throughout the 67 and 20 days storage period.					
36076415	1	60	theme	mediated	141:148	arg1	process					162:168	enzyme mediated degradation process	134:168	enzyme mediated degradation process determined by the action of cell wall modifying enzymes	134:224	Fruit softening is enzyme mediated degradation process determined by the action of cell wall modifying enzymes.					
36116594	0	0	theme	engineering	91:101	arg1	applications					103:114	bone tissue engineering applications	79:114	bone tissue engineering applications	79:114	Effect of cellulose nanofibers on polyhydroxybutyrate electrospun scaffold for bone tissue engineering applications.					
36116594	6	1	theme	cellulose	1202:1210	arg1	presence					1190:1197	the presence	1186:1197	the presence of cellulose nano-additive	1186:1224	Eventually, not only the MTT assay revealed better human osteoblast MG63 cell viability on PC samples, but also DAPI staining and SEM results confirmed the more plausible cell spreading in the presence of cellulose nano-additive.					
36116594	7	2	theme	ALP	1285:1287	arg1	results					1274:1280	the appropriate results	1258:1280	the appropriate results of ALP and Alizarin red	1258:1304	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36116594	4	3	from	angle	646:650	arg1	factor					828:833	toughness factor	818:833	toughness factor of PC	818:839	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	0	4	theme	tissue	84:89	arg1	applications					103:114	bone tissue engineering applications	79:114	bone tissue engineering applications	79:114	Effect of cellulose nanofibers on polyhydroxybutyrate electrospun scaffold for bone tissue engineering applications.					
36116594	7	5	theme	red	1302:1304	arg1	Alizarin					1293:1300	Alizarin red	1293:1304	Alizarin red	1293:1304	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36116594	4	6	theme	Ca/P	771:774	arg1	ratio					776:780	Ca/P ratio	771:780	Ca/P ratio about 1.50	771:791	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	7	dep	rate	696:699	arg1	loss					721:724	up to ≃25 % weight loss	702:724	up to ≃25 % weight loss in two months	702:738	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	8	from	increment	571:579	arg1	%					614:614	≃46 to 53 %	604:614	≃46 to 53 %	604:614	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	6	9	theme	better	1041:1046	arg1	viability					1075:1083	better human osteoblast MG63 cell viability	1041:1083	better human osteoblast MG63 cell viability	1041:1083	Eventually, not only the MTT assay revealed better human osteoblast MG63 cell viability on PC samples, but also DAPI staining and SEM results confirmed the more plausible cell spreading in the presence of cellulose nano-additive.					
36116594	6	10	theme	MTT	1022:1024	arg1	assay					1026:1030	not only the MTT assay	1009:1030	not only the MTT assay	1009:1030	Eventually, not only the MTT assay revealed better human osteoblast MG63 cell viability on PC samples, but also DAPI staining and SEM results confirmed the more plausible cell spreading in the presence of cellulose nano-additive.					
36116594	4	11	theme	neat	856:859	arg1	PHB					861:863	the neat PHB	852:863	the neat PHB	852:863	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	5	12	from	roughness	888:896	arg1	behavior					932:939	cell behavior	927:939	cell behavior	927:939	Moreover, the surface roughness as an affecting parameter on cell behavior was also increased up to ≃43 % in the presence of CNFs.					
36116594	4	13	theme	slight	564:569	arg1	increment					571:579	a slight increment	562:579	a slight increment of crystallinity (from ≃46 to 53 %)	562:615	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	14	dep	increment	805:813	arg1	compare					841:847	compare	841:847	compare to the neat PHB	841:863	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	15	from	biomineralization	752:768	arg1	factor					828:833	toughness factor	818:833	toughness factor of PC	818:839	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	16	theme	prominent	742:750	arg1	biomineralization					752:768	prominent biomineralization	742:768	prominent biomineralization (Ca/P ratio about 1.50)	742:792	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	17	from	rate	696:699	arg1	factor					828:833	toughness factor	818:833	toughness factor of PC	818:839	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	18	theme	better	625:630	arg1	angle					646:650	better water contact angle	625:650	better water contact angle (from ≃120 to 96°)	625:669	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	1	19	theme	important	178:186	arg1	factors					188:194	the most important factors	169:194	the most important factors affecting the final characteristics of the scaffolds	169:247	The choice of materials and preparation methods are the most important factors affecting the final characteristics of the scaffolds.					
36116594	1	19	theme	important	178:186	arg1	choice					121:126	The choice	117:126	The choice of materials and preparation methods	117:163	The choice of materials and preparation methods are the most important factors affecting the final characteristics of the scaffolds.					
36116594	0	20	theme	electrospun	54:64	arg1	scaffold					66:73	polyhydroxybutyrate electrospun scaffold	34:73	polyhydroxybutyrate electrospun scaffold for bone tissue engineering applications	34:114	Effect of cellulose nanofibers on polyhydroxybutyrate electrospun scaffold for bone tissue engineering applications.					
36116594	4	21	theme	crystallinity	584:596	arg1	increment					571:579	a slight increment	562:579	a slight increment of crystallinity (from ≃46 to 53 %)	562:615	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	6	22	dep	staining	1114:1121	arg1	results					1131:1137	results	1131:1137	results	1131:1137	Eventually, not only the MTT assay revealed better human osteoblast MG63 cell viability on PC samples, but also DAPI staining and SEM results confirmed the more plausible cell spreading in the presence of cellulose nano-additive.					
36116594	3	23	theme	pure	515:518	arg1	PHB					520:522	pure PHB	515:522	pure PHB	515:522	The PHB/CNF (PC) scaffold properties, created via the electrospinning method, were investigated and compared with pure PHB.					
36116594	6	24	theme	PC	1088:1089	arg1	samples					1091:1097	PC samples	1088:1097	PC samples	1088:1097	Eventually, not only the MTT assay revealed better human osteoblast MG63 cell viability on PC samples, but also DAPI staining and SEM results confirmed the more plausible cell spreading in the presence of cellulose nano-additive.					
36116594	4	25	dep	96°	666:668	arg1	to					663:664	to	663:664	to	663:664	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	7	26	theme	Alizarin	1293:1300	arg1	results					1274:1280	the appropriate results	1258:1280	the appropriate results of ALP and Alizarin red	1258:1304	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36116594	2	27	theme	nanocomposite	382:394	arg1	mat					396:398	nanocomposite mat	382:398	nanocomposite mat	382:398	In this study, cellulose nanofibers (CNFs) as a nano-additive reinforcer were selected to prepare a polyhydroxybutyrate (PHB) based nanocomposite mat.					
36116594	4	28	dep	biomineralization	752:768	arg1	ratio					776:780	Ca/P ratio	771:780	Ca/P ratio about 1.50	771:791	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	7	29	theme	appropriate	1262:1272	arg1	results					1274:1280	the appropriate results	1258:1280	the appropriate results of ALP and Alizarin red	1258:1304	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36116594	7	30	theme	PC	1335:1336	arg1	composition					1352:1362	the newly PC nanocomposite composition	1325:1362	the newly PC nanocomposite composition	1325:1362	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36116594	4	31	from	%	614:614	arg1	crystallinity					584:596	crystallinity	584:596	crystallinity (from ≃46 to 53 %)	584:615	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	31	from	%	614:614	arg1	increment					571:579	a slight increment	562:579	a slight increment of crystallinity (from ≃46 to 53 %)	562:615	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	3	32	theme	scaffold	418:425	arg1	properties					427:436	The PHB/CNF (PC) scaffold properties	401:436	The PHB/CNF (PC) scaffold properties	401:436	The PHB/CNF (PC) scaffold properties, created via the electrospinning method, were investigated and compared with pure PHB.					
36116594	6	33	theme	cell	1168:1171	arg1	spreading					1173:1181	the more plausible cell spreading	1149:1181	the more plausible cell spreading	1149:1181	Eventually, not only the MTT assay revealed better human osteoblast MG63 cell viability on PC samples, but also DAPI staining and SEM results confirmed the more plausible cell spreading in the presence of cellulose nano-additive.					
36116594	6	34	theme	DAPI	1109:1112	arg1	staining					1114:1121	DAPI staining	1109:1121	DAPI staining	1109:1121	Eventually, not only the MTT assay revealed better human osteoblast MG63 cell viability on PC samples, but also DAPI staining and SEM results confirmed the more plausible cell spreading in the presence of cellulose nano-additive.					
36116594	2	35	theme	nano-additive	298:310	arg1	reinforcer					312:321	a nano-additive reinforcer	296:321	a nano-additive reinforcer	296:321	In this study, cellulose nanofibers (CNFs) as a nano-additive reinforcer were selected to prepare a polyhydroxybutyrate (PHB) based nanocomposite mat.					
36116594	7	36	theme	tissue	1413:1418	arg1	engineering					1420:1430	bone tissue engineering	1408:1430	bone tissue engineering	1408:1430	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36116594	1	37	theme	final	210:214	arg1	characteristics					216:230	the final characteristics	206:230	the final characteristics of the scaffolds	206:247	The choice of materials and preparation methods are the most important factors affecting the final characteristics of the scaffolds.					
36116594	0	38	theme	cellulose	10:18	arg1	Effect					0:5	Effect	0:5	Effect of cellulose	0:18	Effect of cellulose nanofibers on polyhydroxybutyrate electrospun scaffold for bone tissue engineering applications.					
36116594	4	39	theme	%	803:803	arg1	increment					805:813	≃89 % increment	799:813	≃89 % increment in toughness factor of PC compare to the neat PHB	799:863	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	40	theme	appropriate	672:682	arg1	rate					696:699	appropriate degradation rate	672:699	appropriate degradation rate (up to ≃25 % weight loss in two months)	672:739	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	3	41	theme	electrospinning	455:469	arg1	method					471:476	the electrospinning method	451:476	the electrospinning method	451:476	The PHB/CNF (PC) scaffold properties, created via the electrospinning method, were investigated and compared with pure PHB.					
36116594	0	42	theme	polyhydroxybutyrate	34:52	arg1	scaffold					66:73	polyhydroxybutyrate electrospun scaffold	34:73	polyhydroxybutyrate electrospun scaffold for bone tissue engineering applications	34:114	Effect of cellulose nanofibers on polyhydroxybutyrate electrospun scaffold for bone tissue engineering applications.					
36116594	6	43	theme	plausible	1158:1166	arg1	spreading					1173:1181	the more plausible cell spreading	1149:1181	the more plausible cell spreading	1149:1181	Eventually, not only the MTT assay revealed better human osteoblast MG63 cell viability on PC samples, but also DAPI staining and SEM results confirmed the more plausible cell spreading in the presence of cellulose nano-additive.					
36116594	4	44	theme	toughness	818:826	arg1	factor					828:833	toughness factor	818:833	toughness factor of PC	818:839	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	7	45	theme	required	1372:1379	arg1	efficiency					1381:1390	the required efficiency	1368:1390	the required efficiency in the field of bone tissue engineering	1368:1430	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36116594	5	46	theme	surface	880:886	arg1	roughness					888:896	the surface roughness	876:896	the surface roughness as an affecting parameter on cell behavior	876:939	Moreover, the surface roughness as an affecting parameter on cell behavior was also increased up to ≃43 % in the presence of CNFs.					
36116594	4	47	dep	≃25 	708:711	arg1	to					705:706	to	705:706	to	705:706	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	3	48	theme	PC	414:415	arg1	properties					427:436	The PHB/CNF (PC) scaffold properties	401:436	The PHB/CNF (PC) scaffold properties	401:436	The PHB/CNF (PC) scaffold properties, created via the electrospinning method, were investigated and compared with pure PHB.					
36116594	5	49	theme	CNFs	991:994	arg1	presence					979:986	the presence	975:986	the presence of CNFs	975:994	Moreover, the surface roughness as an affecting parameter on cell behavior was also increased up to ≃43 % in the presence of CNFs.					
36116594	4	50	from	increment	805:813	arg1	factor					828:833	toughness factor	818:833	toughness factor of PC	818:839	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	51	dep	53 	611:613	arg1	to					608:609	to	608:609	to	608:609	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	52	theme	PC	838:839	arg1	factor					828:833	toughness factor	818:833	toughness factor of PC	818:839	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	5	53	dep	≃43 	966:969	arg1	to					963:964	to	963:964	to	963:964	Moreover, the surface roughness as an affecting parameter on cell behavior was also increased up to ≃43 % in the presence of CNFs.					
36116594	6	54	theme	MG63	1065:1068	arg1	viability					1075:1083	better human osteoblast MG63 cell viability	1041:1083	better human osteoblast MG63 cell viability	1041:1083	Eventually, not only the MTT assay revealed better human osteoblast MG63 cell viability on PC samples, but also DAPI staining and SEM results confirmed the more plausible cell spreading in the presence of cellulose nano-additive.					
36116594	3	55	theme	PHB/CNF	405:411	arg1	properties					427:436	The PHB/CNF (PC) scaffold properties	401:436	The PHB/CNF (PC) scaffold properties	401:436	The PHB/CNF (PC) scaffold properties, created via the electrospinning method, were investigated and compared with pure PHB.					
36116594	4	56	theme	obtained	529:536	arg1	results					538:544	The obtained results	525:544	The obtained results	525:544	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	7	57	from	efficiency	1381:1390	arg1	field					1399:1403	the field	1395:1403	the field of bone tissue engineering	1395:1430	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36116594	5	58	theme	affecting	904:912	arg1	parameter					914:922	an affecting parameter	901:922	an affecting parameter	901:922	Moreover, the surface roughness as an affecting parameter on cell behavior was also increased up to ≃43 % in the presence of CNFs.					
36116594	6	59	theme	osteoblast	1054:1063	arg1	viability					1075:1083	better human osteoblast MG63 cell viability	1041:1083	better human osteoblast MG63 cell viability	1041:1083	Eventually, not only the MTT assay revealed better human osteoblast MG63 cell viability on PC samples, but also DAPI staining and SEM results confirmed the more plausible cell spreading in the presence of cellulose nano-additive.					
36116594	1	60	theme	materials	131:139	arg1	factors					188:194	the most important factors	169:194	the most important factors affecting the final characteristics of the scaffolds	169:247	The choice of materials and preparation methods are the most important factors affecting the final characteristics of the scaffolds.					
36116594	1	60	theme	materials	131:139	arg1	choice					121:126	The choice	117:126	The choice of materials and preparation methods	117:163	The choice of materials and preparation methods are the most important factors affecting the final characteristics of the scaffolds.					
36116594	4	61	from	96°	666:668	arg1	angle					646:650	better water contact angle	625:650	better water contact angle (from ≃120 to 96°)	625:669	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	61	from	96°	666:668	arg1	increment					805:813	≃89 % increment	799:813	≃89 % increment in toughness factor of PC compare to the neat PHB	799:863	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	61	from	96°	666:668	arg1	biomineralization					752:768	prominent biomineralization	742:768	prominent biomineralization (Ca/P ratio about 1.50)	742:792	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	61	from	96°	666:668	arg1	rate					696:699	appropriate degradation rate	672:699	appropriate degradation rate (up to ≃25 % weight loss in two months)	672:739	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	62	theme	≃89 	799:802	arg1	increment					805:813	≃89 % increment	799:813	≃89 % increment in toughness factor of PC compare to the neat PHB	799:863	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	1	63	theme	methods	157:163	arg1	factors					188:194	the most important factors	169:194	the most important factors affecting the final characteristics of the scaffolds	169:247	The choice of materials and preparation methods are the most important factors affecting the final characteristics of the scaffolds.					
36116594	1	63	theme	methods	157:163	arg1	choice					121:126	The choice	117:126	The choice of materials and preparation methods	117:163	The choice of materials and preparation methods are the most important factors affecting the final characteristics of the scaffolds.					
36116594	4	64	theme	%	712:712	arg1	loss					721:724	up to ≃25 % weight loss	702:724	up to ≃25 % weight loss in two months	702:738	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	6	65	theme	cell	1070:1073	arg1	viability					1075:1083	better human osteoblast MG63 cell viability	1041:1083	better human osteoblast MG63 cell viability	1041:1083	Eventually, not only the MTT assay revealed better human osteoblast MG63 cell viability on PC samples, but also DAPI staining and SEM results confirmed the more plausible cell spreading in the presence of cellulose nano-additive.					
36116594	4	66	theme	degradation	684:694	arg1	rate					696:699	appropriate degradation rate	672:699	appropriate degradation rate (up to ≃25 % weight loss in two months)	672:739	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	1	67	theme	scaffolds	239:247	arg1	characteristics					216:230	the final characteristics	206:230	the final characteristics of the scaffolds	206:247	The choice of materials and preparation methods are the most important factors affecting the final characteristics of the scaffolds.					
36116594	6	68	theme	human	1048:1052	arg1	viability					1075:1083	better human osteoblast MG63 cell viability	1041:1083	better human osteoblast MG63 cell viability	1041:1083	Eventually, not only the MTT assay revealed better human osteoblast MG63 cell viability on PC samples, but also DAPI staining and SEM results confirmed the more plausible cell spreading in the presence of cellulose nano-additive.					
36116594	2	69	theme	cellulose	265:273	arg1	CNFs					287:290	CNFs	287:290	CNFs	287:290	In this study, cellulose nanofibers (CNFs) as a nano-additive reinforcer were selected to prepare a polyhydroxybutyrate (PHB) based nanocomposite mat.					
36116594	2	69	theme	cellulose	265:273	arg1	nanofibers					275:284	cellulose nanofibers	265:284	cellulose nanofibers (CNFs) as a nano-additive reinforcer	265:321	In this study, cellulose nanofibers (CNFs) as a nano-additive reinforcer were selected to prepare a polyhydroxybutyrate (PHB) based nanocomposite mat.					
36116594	4	70	from	loss	721:724	arg1	months					733:738	two months	729:738	two months	729:738	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	1	71	theme	preparation	145:155	arg1	methods					157:163	preparation methods	145:163	preparation methods	145:163	The choice of materials and preparation methods are the most important factors affecting the final characteristics of the scaffolds.					
36116594	7	72	theme	engineering	1420:1430	arg1	field					1399:1403	the field	1395:1403	the field of bone tissue engineering	1395:1430	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36116594	0	73	theme	bone	79:82	arg1	applications					103:114	bone tissue engineering applications	79:114	bone tissue engineering applications	79:114	Effect of cellulose nanofibers on polyhydroxybutyrate electrospun scaffold for bone tissue engineering applications.					
36116594	5	74	theme	cell	927:930	arg1	behavior					932:939	cell behavior	927:939	cell behavior	927:939	Moreover, the surface roughness as an affecting parameter on cell behavior was also increased up to ≃43 % in the presence of CNFs.					
36116594	7	75	contain	has	1364:1366	arg2	efficiency					1381:1390	the required efficiency	1368:1390	the required efficiency in the field of bone tissue engineering	1368:1430	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36116594	7	75	contain	has	1364:1366	arg1	composition					1352:1362	the newly PC nanocomposite composition	1325:1362	the newly PC nanocomposite composition	1325:1362	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36116594	4	76	theme	contact	638:644	arg1	angle					646:650	better water contact angle	625:650	better water contact angle (from ≃120 to 96°)	625:669	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	4	77	theme	weight	714:719	arg1	loss					721:724	up to ≃25 % weight loss	702:724	up to ≃25 % weight loss in two months	702:738	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	7	78	theme	nanocomposite	1338:1350	arg1	composition					1352:1362	the newly PC nanocomposite composition	1325:1362	the newly PC nanocomposite composition	1325:1362	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36116594	4	79	theme	water	632:636	arg1	angle					646:650	better water contact angle	625:650	better water contact angle (from ≃120 to 96°)	625:669	The obtained results, in addition to a slight increment of crystallinity (from ≃46 to 53 %), showed better water contact angle (from ≃120 to 96°), appropriate degradation rate (up to ≃25 % weight loss in two months), prominent biomineralization (Ca/P ratio about 1.50), and ≃89 % increment in toughness factor of PC compare to the neat PHB.					
36116594	7	80	theme	bone	1408:1411	arg1	engineering					1420:1430	bone tissue engineering	1408:1430	bone tissue engineering	1408:1430	These improvements, along with the appropriate results of ALP and Alizarin red, authenticate that the newly PC nanocomposite composition has the required efficiency in the field of bone tissue engineering.					
36053563	3	0	theme	successful	345:354	arg1	synthesis					369:377	The successful vacuum-based synthesis	341:377	The successful vacuum-based synthesis of a glucose-responsive hydrogel	341:410	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	9	1	from	pH	1213:1214	arg1	state					1204:1208	its shrunken state	1191:1208	its shrunken state	1191:1208	The hydrogel was in its shrunken state at pH 4-7 and swelled by increasing the pH to 9.					
36053563	0	2	theme	Vapor	84:88	arg1	Deposition					90:99	Initiated Chemical Vapor Deposition	65:99	Initiated Chemical Vapor Deposition	65:99	Glucose-Responsive Boronic Acid Hydrogel Thin Films Obtained via Initiated Chemical Vapor Deposition.					
36053563	7	3	theme	swelling	941:948	arg1	compound					1001:1008	a glucose-fructose compound	982:1008	a glucose-fructose compound	982:1008	The thin film was characterized in terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound.					
36053563	7	3	theme	swelling	941:948	arg1	response					950:957	swelling response	941:957	swelling response toward pH and sucrose	941:979	The thin film was characterized in terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound.					
36053563	6	4	theme	boronic	771:777	arg1	compound					784:791	a boronic acid compound	769:791	a boronic acid compound	769:791	A hydrogel made of but-3-enylboronic acid units, a boronic acid compound, was synthesized via initiated chemical vapor deposition.					
36053563	6	4	theme	boronic	771:777	arg1	hydrogel					722:729	A hydrogel	720:729	A hydrogel made of but-3-enylboronic acid units	720:766	A hydrogel made of but-3-enylboronic acid units, a boronic acid compound, was synthesized via initiated chemical vapor deposition.					
36053563	7	5	theme	thin	855:858	arg1	film					860:863	The thin film	851:863	The thin film	851:863	The thin film was characterized in terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound.					
36053563	3	6	theme	hydrogel	403:410	arg1	synthesis					369:377	The successful vacuum-based synthesis	341:377	The successful vacuum-based synthesis of a glucose-responsive hydrogel	341:410	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	7	7	theme	response	950:957	arg1	terms					886:890	terms	886:890	terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound	886:1008	The thin film was characterized in terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound.					
36053563	2	8	theme	hydrogel	321:328	arg1	film					335:338	a glucose-responsive hydrogel thin film	300:338	a glucose-responsive hydrogel thin film	300:338	This study presents the first vacuum-based deposition of a glucose-responsive hydrogel thin film.					
36053563	4	9	theme	leachables	599:608	arg1	free					591:594	free	591:594	free	591:594	In addition, vacuum-deposited films are free of leachables (e.g., plasticizers and residual solvents).					
36053563	2	10	theme	glucose-responsive	302:319	arg1	film					335:338	a glucose-responsive hydrogel thin film	300:338	a glucose-responsive hydrogel thin film	300:338	This study presents the first vacuum-based deposition of a glucose-responsive hydrogel thin film.					
36053563	5	11	from	safe	689:692	arg1	principle					678:686	principle	678:686	principle	678:686	Therefore, they are, in principle, safe for in-body applications.					
36053563	4	12	from	free	591:594	arg1	addition					554:561	addition	554:561	addition	554:561	In addition, vacuum-deposited films are free of leachables (e.g., plasticizers and residual solvents).					
36053563	8	13	theme	texture	1138:1144	arg1	appearance					1146:1155	an undulating texture appearance	1124:1155	an undulating texture appearance (rms 2.1 nm)	1124:1168	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	8	13	theme	texture	1138:1144	arg1	nm					1166:1167	rms 2.1 nm	1158:1167	rms 2.1 nm	1158:1167	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	6	14	theme	acid	779:782	arg1	compound					784:791	a boronic acid compound	769:791	a boronic acid compound	769:791	A hydrogel made of but-3-enylboronic acid units, a boronic acid compound, was synthesized via initiated chemical vapor deposition.					
36053563	6	14	theme	acid	779:782	arg1	hydrogel					722:729	A hydrogel	720:729	A hydrogel made of but-3-enylboronic acid units	720:766	A hydrogel made of but-3-enylboronic acid units, a boronic acid compound, was synthesized via initiated chemical vapor deposition.					
36053563	3	15	theme	possible	530:537	arg1	substrates					539:548	new possible substrates	526:548	new possible substrates	526:548	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	3	16	theme	new	451:453	arg1	applications					455:466	new applications	451:466	new applications	451:466	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	3	17	theme	glucose-responsive	384:401	arg1	hydrogel					403:410	a glucose-responsive hydrogel	382:410	a glucose-responsive hydrogel	382:410	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	7	18	theme	glucose-fructose	984:999	arg1	response					950:957	swelling response	941:957	swelling response toward pH and sucrose	941:979	The thin film was characterized in terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound.					
36053563	7	18	theme	glucose-fructose	984:999	arg1	compound					1001:1008	a glucose-fructose compound	982:1008	a glucose-fructose compound	982:1008	The thin film was characterized in terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound.					
36053563	1	19	from	importance	144:153	arg1	field					162:166	the field	158:166	the field of monitoring the physiological glucose level or smart insulin management	158:240	Glucose-responsive materials are of great importance in the field of monitoring the physiological glucose level or smart insulin management.					
36053563	1	20	theme	physiological	186:198	arg1	level					208:212	the physiological glucose level	182:212	the physiological glucose level	182:212	Glucose-responsive materials are of great importance in the field of monitoring the physiological glucose level or smart insulin management.					
36053563	0	21	theme	Boronic	19:25	arg1	Films					46:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films	0:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films	0:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films Obtained via Initiated Chemical Vapor Deposition.					
36053563	3	22	theme	vacuum-based	356:367	arg1	synthesis					369:377	The successful vacuum-based synthesis	341:377	The successful vacuum-based synthesis of a glucose-responsive hydrogel	341:410	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	3	23	dep	variety	440:446	arg1	applied					515:521	applied	515:521	is applied on new possible substrates	512:548	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	7	24	theme	morphology	925:934	arg1	terms					886:890	terms	886:890	terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound	886:1008	The thin film was characterized in terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound.					
36053563	1	25	theme	glucose	200:206	arg1	level					208:212	the physiological glucose level	182:212	the physiological glucose level	182:212	Glucose-responsive materials are of great importance in the field of monitoring the physiological glucose level or smart insulin management.					
36053563	0	26	theme	Glucose-Responsive	0:17	arg1	Films					46:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films	0:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films	0:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films Obtained via Initiated Chemical Vapor Deposition.					
36053563	8	27	theme	aqueous	1034:1040	arg1	solutions					1042:1050	aqueous solutions	1034:1050	aqueous solutions	1034:1050	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	7	28	theme	composition	904:914	arg1	terms					886:890	terms	886:890	terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound	886:1008	The thin film was characterized in terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound.					
36053563	1	29	theme	Glucose-responsive	102:119	arg1	materials					121:129	Glucose-responsive materials	102:129	Glucose-responsive materials	102:129	Glucose-responsive materials are of great importance in the field of monitoring the physiological glucose level or smart insulin management.					
36053563	3	30	theme	applications	455:466	arg1	variety					440:446	a vast variety	433:446	a vast variety	433:446	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	3	30	theme	applications	455:466	arg1	applications					455:466	new applications	451:466	new applications	451:466	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	0	31	theme	Hydrogel	32:39	arg1	Films					46:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films	0:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films	0:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films Obtained via Initiated Chemical Vapor Deposition.					
36053563	2	32	theme	film	335:338	arg1	deposition					286:295	the first vacuum-based deposition	263:295	the first vacuum-based deposition of a glucose-responsive hydrogel thin film	263:338	This study presents the first vacuum-based deposition of a glucose-responsive hydrogel thin film.					
36053563	4	33	theme	residual	634:641	arg1	solvents					643:650	residual solvents	634:650	residual solvents	634:650	In addition, vacuum-deposited films are free of leachables (e.g., plasticizers and residual solvents).					
36053563	0	34	theme	Acid	27:30	arg1	Films					46:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films	0:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films	0:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films Obtained via Initiated Chemical Vapor Deposition.					
36053563	6	35	theme	chemical	824:831	arg1	deposition					839:848	initiated chemical vapor deposition	814:848	initiated chemical vapor deposition	814:848	A hydrogel made of but-3-enylboronic acid units, a boronic acid compound, was synthesized via initiated chemical vapor deposition.					
36053563	8	36	contain	had	1120:1122	arg1	film					1015:1018	The film	1011:1018	The film	1011:1018	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	8	36	contain	had	1120:1122	arg2	appearance					1146:1155	an undulating texture appearance	1124:1155	an undulating texture appearance (rms 2.1 nm)	1124:1168	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	8	36	contain	had	1120:1122	arg2	nm					1166:1167	rms 2.1 nm	1158:1167	rms 2.1 nm	1158:1167	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	8	36	contain	had	1120:1122	arg1	stable					1024:1029	stable	1024:1029	stable	1024:1029	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	4	37	dep	leachables	599:608	arg1	solvents					643:650	residual solvents	634:650	residual solvents	634:650	In addition, vacuum-deposited films are free of leachables (e.g., plasticizers and residual solvents).					
36053563	4	37	dep	leachables	599:608	arg1	plasticizers					617:628	plasticizers	617:628	plasticizers	617:628	In addition, vacuum-deposited films are free of leachables (e.g., plasticizers and residual solvents).					
36053563	2	38	theme	thin	330:333	arg1	film					335:338	a glucose-responsive hydrogel thin film	300:338	a glucose-responsive hydrogel thin film	300:338	This study presents the first vacuum-based deposition of a glucose-responsive hydrogel thin film.					
36053563	1	39	theme	smart	217:221	arg1	management					231:240	smart insulin management	217:240	smart insulin management	217:240	Glucose-responsive materials are of great importance in the field of monitoring the physiological glucose level or smart insulin management.					
36053563	11	40	theme	thickness	1362:1370	arg1	shrinking					1372:1380	thickness shrinking	1362:1380	thickness shrinking	1362:1380	The response to glucose was observed at pH 10 and resulted in thickness shrinking.					
36053563	4	41	theme	vacuum-deposited	564:579	arg1	films					581:585	vacuum-deposited films	564:585	vacuum-deposited films	564:585	In addition, vacuum-deposited films are free of leachables (e.g., plasticizers and residual solvents).					
36053563	8	42	theme	polymerized	1067:1077	arg1	acid					1087:1090	polymerized boronic acid	1067:1090	polymerized boronic acid	1067:1090	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	8	43	theme	rms	1158:1160	arg1	appearance					1146:1155	an undulating texture appearance	1124:1155	an undulating texture appearance (rms 2.1 nm)	1124:1168	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	8	43	theme	rms	1158:1160	arg1	nm					1166:1167	rms 2.1 nm	1158:1167	rms 2.1 nm	1158:1167	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	1	44	theme	insulin	223:229	arg1	management					231:240	smart insulin management	217:240	smart insulin management	217:240	Glucose-responsive materials are of great importance in the field of monitoring the physiological glucose level or smart insulin management.					
36053563	0	45	theme	Thin	41:44	arg1	Films					46:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films	0:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films	0:50	Glucose-Responsive Boronic Acid Hydrogel Thin Films Obtained via Initiated Chemical Vapor Deposition.					
36053563	8	46	from	solutions	1042:1050	arg1	film					1015:1018	The film	1011:1018	The film	1011:1018	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	8	46	from	solutions	1042:1050	arg1	stable					1024:1029	stable	1024:1029	stable	1024:1029	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	5	47	theme	in-body	698:704	arg1	applications					706:717	in-body applications	698:717	in-body applications	698:717	Therefore, they are, in principle, safe for in-body applications.					
36053563	11	48	located	observed	1328:1335	arg1	pH					1340:1341	pH 10	1340:1344	pH 10	1340:1344	The response to glucose was observed at pH 10 and resulted in thickness shrinking.					
36053563	11	48	located	observed	1328:1335	arg2	response					1304:1311	The response	1300:1311	The response to glucose	1300:1322	The response to glucose was observed at pH 10 and resulted in thickness shrinking.					
36053563	6	49	theme	acid	757:760	arg1	units					762:766	but-3-enylboronic acid units	739:766	but-3-enylboronic acid units	739:766	A hydrogel made of but-3-enylboronic acid units, a boronic acid compound, was synthesized via initiated chemical vapor deposition.					
36053563	8	50	theme	undulating	1127:1136	arg1	appearance					1146:1155	an undulating texture appearance	1124:1155	an undulating texture appearance (rms 2.1 nm)	1124:1168	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	8	50	theme	undulating	1127:1136	arg1	nm					1166:1167	rms 2.1 nm	1158:1167	rms 2.1 nm	1158:1167	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	6	51	theme	vapor	833:837	arg1	deposition					839:848	initiated chemical vapor deposition	814:848	initiated chemical vapor deposition	814:848	A hydrogel made of but-3-enylboronic acid units, a boronic acid compound, was synthesized via initiated chemical vapor deposition.					
36053563	6	52	theme	but-3-enylboronic	739:755	arg1	units					762:766	but-3-enylboronic acid units	739:766	but-3-enylboronic acid units	739:766	A hydrogel made of but-3-enylboronic acid units, a boronic acid compound, was synthesized via initiated chemical vapor deposition.					
36053563	3	53	theme	thin	502:505	arg1	film					507:510	the hydrogel thin film	489:510	the hydrogel thin film	489:510	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	3	54	theme	vast	435:438	arg1	variety					440:446	a vast variety	433:446	a vast variety	433:446	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	3	54	theme	vast	435:438	arg1	applications					455:466	new applications	451:466	new applications	451:466	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	9	55	theme	shrunken	1195:1202	arg1	state					1204:1208	its shrunken state	1191:1208	its shrunken state	1191:1208	The hydrogel was in its shrunken state at pH 4-7 and swelled by increasing the pH to 9.					
36053563	8	56	theme	boronic	1079:1085	arg1	acid					1087:1090	polymerized boronic acid	1067:1090	polymerized boronic acid	1067:1090	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	4	57	dep	plasticizers	617:628	arg1	e.g.					611:614	e.g.	611:614	e.g.	611:614	In addition, vacuum-deposited films are free of leachables (e.g., plasticizers and residual solvents).					
36053563	7	58	theme	surface	917:923	arg1	morphology					925:934	surface morphology	917:934	surface morphology	917:934	The thin film was characterized in terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound.					
36053563	1	59	theme	great	138:142	arg1	importance					144:153	great importance	138:153	great importance	138:153	Glucose-responsive materials are of great importance in the field of monitoring the physiological glucose level or smart insulin management.					
36053563	8	60	from	stable	1024:1029	arg1	solutions					1042:1050	aqueous solutions	1034:1050	aqueous solutions	1034:1050	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	6	61	theme	initiated	814:822	arg1	deposition					839:848	initiated chemical vapor deposition	814:848	initiated chemical vapor deposition	814:848	A hydrogel made of but-3-enylboronic acid units, a boronic acid compound, was synthesized via initiated chemical vapor deposition.					
36053563	7	62	theme	chemical	895:902	arg1	composition					904:914	chemical composition	895:914	chemical composition	895:914	The thin film was characterized in terms of chemical composition, surface morphology, and swelling response toward pH and sucrose, a glucose-fructose compound.					
36053563	2	63	theme	vacuum-based	273:284	arg1	deposition					286:295	the first vacuum-based deposition	263:295	the first vacuum-based deposition of a glucose-responsive hydrogel thin film	263:338	This study presents the first vacuum-based deposition of a glucose-responsive hydrogel thin film.					
36053563	3	64	theme	hydrogel	493:500	arg1	film					507:510	the hydrogel thin film	489:510	the hydrogel thin film	489:510	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36053563	8	65	theme	initiator	1100:1108	arg1	unit					1110:1113	the initiator unit	1096:1113	the initiator unit	1096:1113	The film was stable in aqueous solutions, consisting of polymerized boronic acid and the initiator unit, and had an undulating texture appearance (rms 2.1 nm).					
36053563	5	66	from	principle	678:686	arg1	safe					689:692	safe	689:692	safe	689:692	Therefore, they are, in principle, safe for in-body applications.					
36053563	0	67	theme	Chemical	75:82	arg1	Deposition					90:99	Initiated Chemical Vapor Deposition	65:99	Initiated Chemical Vapor Deposition	65:99	Glucose-Responsive Boronic Acid Hydrogel Thin Films Obtained via Initiated Chemical Vapor Deposition.					
36053563	2	68	theme	first	267:271	arg1	deposition					286:295	the first vacuum-based deposition	263:295	the first vacuum-based deposition of a glucose-responsive hydrogel thin film	263:338	This study presents the first vacuum-based deposition of a glucose-responsive hydrogel thin film.					
36053563	0	69	theme	Initiated	65:73	arg1	Deposition					90:99	Initiated Chemical Vapor Deposition	65:99	Initiated Chemical Vapor Deposition	65:99	Glucose-Responsive Boronic Acid Hydrogel Thin Films Obtained via Initiated Chemical Vapor Deposition.					
36053563	4	70	from	addition	554:561	arg1	free					591:594	free	591:594	free	591:594	In addition, vacuum-deposited films are free of leachables (e.g., plasticizers and residual solvents).					
36053563	3	71	theme	new	526:528	arg1	substrates					539:548	new possible substrates	526:548	new possible substrates	526:548	The successful vacuum-based synthesis of a glucose-responsive hydrogel may open the door to a vast variety of new applications, where, for example, the hydrogel thin film is applied on new possible substrates.					
36801283	7	0	theme	Langmuir	1418:1425	arg1	isotherm					1427:1434	Langmuir isotherm	1418:1434	Langmuir isotherm	1418:1434	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	2	1	theme	35.147 m2 g-1	562:574	arg1	surface					551:557	a surface	549:557	a surface of 35.147 m2 g-1 of luminescent fibers	549:596	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	0	2	theme	ions	129:132	arg1	detection					97:105	detection	97:105	detection	97:105	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	0	2	theme	ions	129:132	arg1	removal					111:117	removal	111:117	removal of Hg(II) ions from waste water	111:149	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	8	3	theme	upto	1549:1552	arg1	5 cycles					1554:1561	recyclability upto 5 cycles	1535:1561	recyclability upto 5 cycles	1535:1561	BNQD@CNFs exhibited recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles, demonstrating high potential in wastewater remediation.					
36801283	6	4	theme	photon	1208:1213	arg1	spectroscopy					1215:1226	X-ray photon spectroscopy	1202:1226	X-ray photon spectroscopy	1202:1226	BNQD@CNFs concomitantly exhibited adsorption of Hg(II) owing to strong electrostatic interactions, confirmed by X-ray photon spectroscopy.					
36801283	1	5	theme	nitride	218:224	arg1	substrate					325:333	substrate	325:333	substrate	325:333	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	1	5	theme	nitride	218:224	arg1	BNQDs					240:244	BNQDs	240:244	BNQDs	240:244	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	1	5	theme	nitride	218:224	arg1	dots					234:237	boron nitride quantum dots	212:237	boron nitride quantum dots (BNQDs)	212:245	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	7	6	theme	314.5 mg/ g.	1337:1348	arg1	studies					1361:1367	314.5 mg/ g. Parametric studies	1337:1367	314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99	1337:1449	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	7	7	with	isotherm	1427:1434	arg1	R2 ≈ 0.99					1441:1449	R2 ≈ 0.99	1441:1449	R2 ≈ 0.99	1441:1449	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	7	8	theme	pseudo-second	1385:1397	arg1	kinetics					1405:1412	pseudo-second order kinetics	1385:1412	pseudo-second order kinetics	1385:1412	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	8	9	theme	wastewater	1596:1605	arg1	remediation					1607:1617	wastewater remediation	1596:1617	wastewater remediation	1596:1617	BNQD@CNFs exhibited recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles, demonstrating high potential in wastewater remediation.					
36801283	4	10	theme	@	830:830	arg1	CNFs					831:834	BNQD@CNFs	826:834	BNQD@CNFs	826:834	The nitrogen-rich surface of BNQD@CNFs exhibited strong affinity for Hg(II), quenching the fluorescence intensity due to combined inner-filter effect and photo-induced electron transfer.					
36801283	7	11	theme	maximum	1306:1312	arg1	capacity					1325:1332	maximum adsorption capacity	1306:1332	maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99	1306:1449	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	6	12	theme	electrostatic	1161:1173	arg1	interactions					1175:1186	strong electrostatic interactions	1154:1186	strong electrostatic interactions	1154:1186	BNQD@CNFs concomitantly exhibited adsorption of Hg(II) owing to strong electrostatic interactions, confirmed by X-ray photon spectroscopy.					
36801283	4	13	theme	CNFs	831:834	arg1	surface					815:821	The nitrogen-rich surface	797:821	The nitrogen-rich surface of BNQD@CNFs	797:834	The nitrogen-rich surface of BNQD@CNFs exhibited strong affinity for Hg(II), quenching the fluorescence intensity due to combined inner-filter effect and photo-induced electron transfer.					
36801283	2	14	theme	BNQD	529:532	arg1	CNFs					534:537	BNQD@CNFs	529:537	BNQD@CNFs	529:537	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	2	14	theme	BNQD	529:532	arg1	CNFs					523:526	CNFs	523:526	CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers	523:596	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	3	15	theme	thermal	708:714	arg1	stability					716:724	high thermal stability	703:724	high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45	703:794	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	5	16	theme	limit	1017:1021	arg1	limit					988:992	The limit	984:992	The limit of detection (LOD) and limit of quantification (LOQ)	984:1045	The limit of detection (LOD) and limit of quantification (LOQ) were 4.889 nM and 11.1 5 nM, respectively.					
36801283	5	16	theme	limit	1017:1021	arg1	5 nM					1070:1073	4.889 nM and 11.1 5 nM	1052:1073	4.889 nM and 11.1 5 nM	1052:1073	The limit of detection (LOD) and limit of quantification (LOQ) were 4.889 nM and 11.1 5 nM, respectively.					
36801283	2	17	theme	CNFs	523:526	arg1	fibrous-network					504:518	fibrous-network	504:518	fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers	504:596	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	6	18	theme	strong	1154:1159	arg1	interactions					1175:1186	strong electrostatic interactions	1154:1186	strong electrostatic interactions	1154:1186	BNQD@CNFs concomitantly exhibited adsorption of Hg(II) owing to strong electrostatic interactions, confirmed by X-ray photon spectroscopy.					
36801283	5	19	theme	quantification	1026:1039	arg1	LOQ					1042:1044	LOQ	1042:1044	LOQ	1042:1044	The limit of detection (LOD) and limit of quantification (LOQ) were 4.889 nM and 11.1 5 nM, respectively.					
36801283	5	19	theme	quantification	1026:1039	arg1	LOD					1008:1010	LOD	1008:1010	LOD	1008:1010	The limit of detection (LOD) and limit of quantification (LOQ) were 4.889 nM and 11.1 5 nM, respectively.					
36801283	5	19	theme	quantification	1026:1039	arg1	limit					1017:1021	limit	1017:1021	limit of quantification (LOQ)	1017:1045	The limit of detection (LOD) and limit of quantification (LOQ) were 4.889 nM and 11.1 5 nM, respectively.					
36801283	5	19	theme	quantification	1026:1039	arg1	detection					997:1005	detection	997:1005	detection (LOD)	997:1011	The limit of detection (LOD) and limit of quantification (LOQ) were 4.889 nM and 11.1 5 nM, respectively.					
36801283	1	20	theme	heavy-metal	180:190	arg1	ions					192:195	heavy-metal ions	180:195	heavy-metal ions in wastewater	180:209	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	8	21	theme	recovery	1472:1479	arg1	rate					1481:1484	recovery rate	1472:1484	recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles	1472:1561	BNQD@CNFs exhibited recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles, demonstrating high potential in wastewater remediation.					
36801283	0	22	theme	waste	139:143	arg1	water					145:149	waste water	139:149	waste water	139:149	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	4	23	theme	inner-filter	927:938	arg1	effect					940:945	combined inner-filter effect	918:945	combined inner-filter effect	918:945	The nitrogen-rich surface of BNQD@CNFs exhibited strong affinity for Hg(II), quenching the fluorescence intensity due to combined inner-filter effect and photo-induced electron transfer.					
36801283	7	24	theme	%	1268:1268	arg1	removal					1270:1276	96 % removal	1265:1276	96 % removal of Hg(II)	1265:1286	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	2	25	theme	extraordinary	452:464	arg1	properties					479:488	the extraordinary fluorescence properties	448:488	the extraordinary fluorescence properties of BNQDs	448:497	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	0	26	theme	cellulose	72:80	arg1	nanofibers					82:91	cellulose nanofibers	72:91	cellulose nanofibers for detection and removal of Hg(II) ions from waste water	72:149	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	1	27	theme	cellulose	294:302	arg1	CNFs					316:319	CNFs	316:319	CNFs	316:319	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	1	27	theme	cellulose	294:302	arg1	nanofibers					304:313	rice straw derived cellulose nanofibers	275:313	rice straw derived cellulose nanofibers (CNFs)	275:320	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	3	28	theme	0.45	791:794	arg1	yield					782:786	quantum yield	774:786	quantum yield	774:786	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	3	28	theme	0.45	791:794	arg1	347.7 °C					761:768	347.7 °C	761:768	347.7 °C	761:768	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	2	29	theme	BNQDs	493:497	arg1	properties					479:488	the extraordinary fluorescence properties	448:488	the extraordinary fluorescence properties of BNQDs	448:497	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	8	30	theme	BNQD	1452:1455	arg1	CNFs					1457:1460	BNQD@CNFs	1452:1460	BNQD@CNFs	1452:1460	BNQD@CNFs exhibited recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles, demonstrating high potential in wastewater remediation.					
36801283	2	31	theme	composite	340:348	arg1	system					350:355	The composite system	336:355	The composite system	336:355	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	7	32	theme	96 	1265:1267	arg1	%					1268:1268	%	1268:1268	%	1268:1268	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	0	33	from	water	145:149	arg1	detection					97:105	detection	97:105	detection	97:105	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	0	33	from	water	145:149	arg1	removal					111:117	removal	111:117	removal of Hg(II) ions from waste water	111:149	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	7	34	theme	bonds	1250:1254	arg1	Presence					1229:1236	Presence	1229:1236	Presence of polar BN bonds	1229:1254	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	6	35	theme	Hg	1138:1139	arg1	adsorption					1124:1133	adsorption	1124:1133	adsorption of Hg(II)	1124:1143	BNQD@CNFs concomitantly exhibited adsorption of Hg(II) owing to strong electrostatic interactions, confirmed by X-ray photon spectroscopy.					
36801283	2	36	theme	hydrophilic-hydrophobic	374:396	arg1	interactions					398:409	strong hydrophilic-hydrophobic interactions	367:409	strong hydrophilic-hydrophobic interactions	367:409	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	7	37	theme	polar	1241:1245	arg1	bonds					1250:1254	polar BN bonds	1241:1254	polar BN bonds	1241:1254	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	4	38	theme	electron	965:972	arg1	transfer					974:981	photo-induced electron transfer	951:981	photo-induced electron transfer	951:981	The nitrogen-rich surface of BNQD@CNFs exhibited strong affinity for Hg(II), quenching the fluorescence intensity due to combined inner-filter effect and photo-induced electron transfer.					
36801283	1	39	dep	derived	286:292	arg1	straw					280:284	rice straw	275:284	rice straw	275:284	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	3	40	theme	uniform	630:636	arg1	distribution					638:649	uniform distribution	630:649	uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45	630:794	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	2	41	dep	exhibited	357:365	arg1	integrated					437:446	integrated	437:446	integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers	437:596	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	1	42	link	derived	286:292	arg1	CNFs					316:319	CNFs	316:319	CNFs	316:319	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	1	42	link	derived	286:292	arg1	nanofibers					304:313	rice straw derived cellulose nanofibers	275:313	rice straw derived cellulose nanofibers (CNFs)	275:320	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	0	43	theme	boron	32:36	arg1	dots					54:57	boron nitride quantum dots	32:57	boron nitride quantum dots	32:57	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	8	44	theme	%	1500:1500	arg1	%					1506:1506	101.3 %-111 %	1494:1506	101.3 %-111 % for real water samples and recyclability upto 5 cycles	1494:1561	BNQD@CNFs exhibited recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles, demonstrating high potential in wastewater remediation.					
36801283	2	45	theme	fluorescence	466:477	arg1	properties					479:488	the extraordinary fluorescence properties	448:488	the extraordinary fluorescence properties of BNQDs	448:497	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	4	46	theme	nitrogen-rich	801:813	arg1	surface					815:821	The nitrogen-rich surface	797:821	The nitrogen-rich surface of BNQD@CNFs	797:834	The nitrogen-rich surface of BNQD@CNFs exhibited strong affinity for Hg(II), quenching the fluorescence intensity due to combined inner-filter effect and photo-induced electron transfer.					
36801283	0	47	theme	quantum	46:52	arg1	dots					54:57	boron nitride quantum dots	32:57	boron nitride quantum dots	32:57	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	5	48	theme	detection	997:1005	arg1	limit					988:992	The limit	984:992	The limit of detection (LOD) and limit of quantification (LOQ)	984:1045	The limit of detection (LOD) and limit of quantification (LOQ) were 4.889 nM and 11.1 5 nM, respectively.					
36801283	5	48	theme	detection	997:1005	arg1	5 nM					1070:1073	4.889 nM and 11.1 5 nM	1052:1073	4.889 nM and 11.1 5 nM	1052:1073	The limit of detection (LOD) and limit of quantification (LOQ) were 4.889 nM and 11.1 5 nM, respectively.					
36801283	1	49	theme	ions	192:195	arg1	challenge					167:175	the challenge	163:175	the challenge of heavy-metal ions in wastewater	163:209	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	8	50	theme	real	1512:1515	arg1	samples					1523:1529	real water samples	1512:1529	real water samples	1512:1529	BNQD@CNFs exhibited recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles, demonstrating high potential in wastewater remediation.					
36801283	3	51	from	distribution	638:649	arg1	CNFs					663:666	CNFs	663:666	CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45	663:794	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	3	52	theme	hydrogen	675:682	arg1	bonding					684:690	hydrogen bonding	675:690	hydrogen bonding	675:690	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	7	53	with	kinetics	1405:1412	arg1	R2 ≈ 0.99					1441:1449	R2 ≈ 0.99	1441:1449	R2 ≈ 0.99	1441:1449	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	1	54	theme	boron	212:216	arg1	substrate					325:333	substrate	325:333	substrate	325:333	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	1	54	theme	boron	212:216	arg1	BNQDs					240:244	BNQDs	240:244	BNQDs	240:244	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	1	54	theme	boron	212:216	arg1	dots					234:237	boron nitride quantum dots	212:237	boron nitride quantum dots (BNQDs)	212:245	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	7	55	theme	order	1399:1403	arg1	kinetics					1405:1412	pseudo-second order kinetics	1385:1412	pseudo-second order kinetics	1385:1412	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	2	56	theme	luminescent	579:589	arg1	fibers					591:596	luminescent fibers	579:596	luminescent fibers	579:596	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	8	57	theme	recyclability	1535:1547	arg1	5 cycles					1554:1561	recyclability upto 5 cycles	1535:1561	recyclability upto 5 cycles	1535:1561	BNQD@CNFs exhibited recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles, demonstrating high potential in wastewater remediation.					
36801283	3	58	theme	due	668:670	arg1	CNFs					663:666	CNFs	663:666	CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45	663:794	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	6	59	theme	X-ray	1202:1206	arg1	spectroscopy					1215:1226	X-ray photon spectroscopy	1202:1226	X-ray photon spectroscopy	1202:1226	BNQD@CNFs concomitantly exhibited adsorption of Hg(II) owing to strong electrostatic interactions, confirmed by X-ray photon spectroscopy.					
36801283	1	60	theme	quantum	226:232	arg1	substrate					325:333	substrate	325:333	substrate	325:333	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	1	60	theme	quantum	226:232	arg1	BNQDs					240:244	BNQDs	240:244	BNQDs	240:244	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	1	60	theme	quantum	226:232	arg1	dots					234:237	boron nitride quantum dots	212:237	boron nitride quantum dots (BNQDs)	212:245	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	6	61	theme	@	1094:1094	arg1	CNFs					1095:1098	BNQD@CNFs	1090:1098	BNQD@CNFs	1090:1098	BNQD@CNFs concomitantly exhibited adsorption of Hg(II) owing to strong electrostatic interactions, confirmed by X-ray photon spectroscopy.					
36801283	4	62	theme	strong	846:851	arg1	affinity					853:860	strong affinity	846:860	strong affinity for Hg(II)	846:871	The nitrogen-rich surface of BNQD@CNFs exhibited strong affinity for Hg(II), quenching the fluorescence intensity due to combined inner-filter effect and photo-induced electron transfer.					
36801283	7	63	theme	Parametric	1350:1359	arg1	studies					1361:1367	314.5 mg/ g. Parametric studies	1337:1367	314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99	1337:1449	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	3	64	theme	high	703:706	arg1	stability					716:724	high thermal stability	703:724	high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45	703:794	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	7	65	theme	studies	1361:1367	arg1	capacity					1325:1332	maximum adsorption capacity	1306:1332	maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99	1306:1449	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	2	66	theme	fibers	591:596	arg1	35.147 m2 g-1					562:574	35.147 m2 g-1	562:574	35.147 m2 g-1 of luminescent fibers	562:596	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	4	67	theme	BNQD	826:829	arg1	CNFs					831:834	BNQD@CNFs	826:834	BNQD@CNFs	826:834	The nitrogen-rich surface of BNQD@CNFs exhibited strong affinity for Hg(II), quenching the fluorescence intensity due to combined inner-filter effect and photo-induced electron transfer.					
36801283	6	68	theme	BNQD	1090:1093	arg1	CNFs					1095:1098	BNQD@CNFs	1090:1098	BNQD@CNFs	1090:1098	BNQD@CNFs concomitantly exhibited adsorption of Hg(II) owing to strong electrostatic interactions, confirmed by X-ray photon spectroscopy.					
36801283	3	69	theme	peak	731:734	arg1	degradation					736:746	peak degradation	731:746	peak degradation occurring at 347.7 °C and quantum yield of 0.45	731:794	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	0	70	theme	Hg	122:123	arg1	ions					129:132	Hg(II) ions	122:132	Hg(II) ions	122:132	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	7	71	theme	adsorption	1314:1323	arg1	capacity					1325:1332	maximum adsorption capacity	1306:1332	maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99	1306:1449	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	1	72	from	wastewater	200:209	arg1	challenge					167:175	the challenge	163:175	the challenge of heavy-metal ions in wastewater	163:209	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	1	73	from	ions	192:195	arg1	wastewater					200:209	wastewater	200:209	wastewater	200:209	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	1	74	from	challenge	167:175	arg1	wastewater					200:209	wastewater	200:209	wastewater	200:209	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	2	75	theme	@	533:533	arg1	CNFs					534:537	BNQD@CNFs	529:537	BNQD@CNFs	529:537	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	2	75	theme	@	533:533	arg1	CNFs					523:526	CNFs	523:526	CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers	523:596	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	7	76	theme	Hg	1281:1282	arg1	removal					1270:1276	96 % removal	1265:1276	96 % removal of Hg(II)	1265:1286	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	4	77	theme	combined	918:925	arg1	effect					940:945	combined inner-filter effect	918:945	combined inner-filter effect	918:945	The nitrogen-rich surface of BNQD@CNFs exhibited strong affinity for Hg(II), quenching the fluorescence intensity due to combined inner-filter effect and photo-induced electron transfer.					
36801283	3	78	theme	quantum	774:780	arg1	yield					782:786	quantum yield	774:786	quantum yield	774:786	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	1	79	theme	derived	286:292	arg1	CNFs					316:319	CNFs	316:319	CNFs	316:319	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	1	79	theme	derived	286:292	arg1	nanofibers					304:313	rice straw derived cellulose nanofibers	275:313	rice straw derived cellulose nanofibers (CNFs)	275:320	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	4	80	theme	fluorescence	888:899	arg1	intensity					901:909	the fluorescence intensity	884:909	the fluorescence intensity	884:909	The nitrogen-rich surface of BNQD@CNFs exhibited strong affinity for Hg(II), quenching the fluorescence intensity due to combined inner-filter effect and photo-induced electron transfer.					
36801283	8	81	theme	@	1456:1456	arg1	CNFs					1457:1460	BNQD@CNFs	1452:1460	BNQD@CNFs	1452:1460	BNQD@CNFs exhibited recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles, demonstrating high potential in wastewater remediation.					
36801283	0	82	theme	fluorescent	7:17	arg1	composite					19:27	Highly fluorescent composite	0:27	Highly fluorescent composite of boron nitride quantum dots	0:57	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	0	82	theme	fluorescent	7:17	arg1	dots					54:57	boron nitride quantum dots	32:57	boron nitride quantum dots	32:57	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	3	83	with	stability	716:724	arg1	degradation					736:746	peak degradation	731:746	peak degradation occurring at 347.7 °C and quantum yield of 0.45	731:794	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	7	84	theme	BN	1247:1248	arg1	bonds					1250:1254	polar BN bonds	1241:1254	polar BN bonds	1241:1254	Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99.					
36801283	8	85	theme	101.3 	1494:1499	arg1	%					1506:1506	101.3 %-111 %	1494:1506	101.3 %-111 % for real water samples and recyclability upto 5 cycles	1494:1561	BNQD@CNFs exhibited recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles, demonstrating high potential in wastewater remediation.					
36801283	2	86	theme	strong	367:372	arg1	interactions					398:409	strong hydrophilic-hydrophobic interactions	367:409	strong hydrophilic-hydrophobic interactions	367:409	The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers.					
36801283	0	87	theme	nitride	38:44	arg1	dots					54:57	boron nitride quantum dots	32:57	boron nitride quantum dots	32:57	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	4	88	theme	photo-induced	951:963	arg1	transfer					974:981	photo-induced electron transfer	951:981	photo-induced electron transfer	951:981	The nitrogen-rich surface of BNQD@CNFs exhibited strong affinity for Hg(II), quenching the fluorescence intensity due to combined inner-filter effect and photo-induced electron transfer.					
36801283	3	89	theme	Morphological	599:611	arg1	studies					613:619	Morphological studies	599:619	Morphological studies	599:619	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	0	90	theme	dots	54:57	arg1	composite					19:27	Highly fluorescent composite	0:27	Highly fluorescent composite of boron nitride quantum dots	0:57	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	0	90	theme	dots	54:57	arg1	dots					54:57	boron nitride quantum dots	32:57	boron nitride quantum dots	32:57	Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.					
36801283	3	91	theme	BNQDs	654:658	arg1	distribution					638:649	uniform distribution	630:649	uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45	630:794	Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45.					
36801283	8	92	theme	high	1578:1581	arg1	potential					1583:1591	high potential	1578:1591	high potential	1578:1591	BNQD@CNFs exhibited recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles, demonstrating high potential in wastewater remediation.					
36801283	1	93	theme	rice	275:278	arg1	straw					280:284	rice straw	275:284	rice straw	275:284	To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate.					
36801283	8	94	theme	water	1517:1521	arg1	samples					1523:1529	real water samples	1512:1529	real water samples	1512:1529	BNQD@CNFs exhibited recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles, demonstrating high potential in wastewater remediation.					
35728638	8	0	theme	photocatalytic	976:989	arg1	efficiency					1003:1012	An excellent photocatalytic degradation efficiency	963:1012	An excellent photocatalytic degradation efficiency for tartrazine and sunset yellow dyes	963:1050	An excellent photocatalytic degradation efficiency for tartrazine and sunset yellow dyes was obtained using CS-ZnSe.					
35728638	13	1	theme	industrial	1879:1888	arg1	wastewater					1890:1899	industrial wastewater	1879:1899	industrial wastewater	1879:1899	Results showed that the CH-ZnS possesses excellent efficiency in decontaminating organic dyes from industrial wastewater.					
35728638	1	2	theme	bodies	286:291	arg1	environment					244:254	environment	244:254	environment	244:254	Employing dyes in different industrial sectors has produced a serious threat to the environment and living organisms of water bodies and land.					
35728638	1	2	theme	bodies	286:291	arg1	organisms					267:275	living organisms	260:275	living organisms	260:275	Employing dyes in different industrial sectors has produced a serious threat to the environment and living organisms of water bodies and land.					
35728638	3	3	theme	reduction	531:539	arg1	method					541:546	chemical reduction method	522:546	chemical reduction method	522:546	In this context, chitosan-zinc selenide (CS-ZnSe) nanoparticles were prepared through chemical reduction method as the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis.					
35728638	8	4	theme	sunset	1033:1038	arg1	dyes					1047:1050	sunset yellow dyes	1033:1050	sunset yellow dyes	1033:1050	An excellent photocatalytic degradation efficiency for tartrazine and sunset yellow dyes was obtained using CS-ZnSe.					
35728638	0	5	theme	sunset	140:145	arg1	dyes					154:157	tartrazine and sunset yellow dyes	125:157	tartrazine and sunset yellow dyes	125:157	Designing, characterization, and evaluation of chitosan-zinc selenide nanoparticles for visible-light-induced degradation of tartrazine and sunset yellow dyes.					
35728638	3	6	theme	efficient	555:563	arg1	photocatalysts					565:578	the efficient photocatalysts	551:578	the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis	551:639	In this context, chitosan-zinc selenide (CS-ZnSe) nanoparticles were prepared through chemical reduction method as the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis.					
35728638	3	6	theme	efficient	555:563	arg1	nanoparticles					486:498	chitosan-zinc selenide (CS-ZnSe) nanoparticles	453:498	chitosan-zinc selenide (CS-ZnSe) nanoparticles	453:498	In this context, chitosan-zinc selenide (CS-ZnSe) nanoparticles were prepared through chemical reduction method as the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis.					
35728638	1	7	theme	serious	222:228	arg1	threat					230:235	a serious threat	220:235	a serious threat to the environment and living organisms of water bodies and land	220:300	Employing dyes in different industrial sectors has produced a serious threat to the environment and living organisms of water bodies and land.					
35728638	10	8	theme	SY	1528:1529	arg1	dyes					1531:1534	SY dyes	1528:1534	SY dyes	1528:1534	Rate constant, 'K' value was found to be 0.001362 min-1 and 0.001257 min-1 for tartrazine and SY dyes, respectively.					
35728638	0	9	theme	dyes	154:157	arg1	degradation					110:120	visible-light-induced degradation	88:120	visible-light-induced degradation of tartrazine and sunset yellow dyes	88:157	Designing, characterization, and evaluation of chitosan-zinc selenide nanoparticles for visible-light-induced degradation of tartrazine and sunset yellow dyes.					
35728638	3	10	theme	toxic	607:611	arg1	dyes					613:616	toxic dyes	607:616	toxic dyes	607:616	In this context, chitosan-zinc selenide (CS-ZnSe) nanoparticles were prepared through chemical reduction method as the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis.					
35728638	1	11	dep	environment	244:254	arg1	the					240:242	the	240:242	the	240:242	Employing dyes in different industrial sectors has produced a serious threat to the environment and living organisms of water bodies and land.					
35728638	4	12	theme	FTIR	699:702	arg1	spectroscopy					704:715	FTIR spectroscopy	699:715	FTIR spectroscopy	699:715	Photocatalyst's synthesis was confirmed with the help of FTIR spectroscopy.					
35728638	13	13	theme	excellent	1821:1829	arg1	efficiency					1831:1840	excellent efficiency	1821:1840	excellent efficiency	1821:1840	Results showed that the CH-ZnS possesses excellent efficiency in decontaminating organic dyes from industrial wastewater.					
35728638	1	14	theme	water	280:284	arg1	bodies					286:291	water bodies	280:291	water bodies	280:291	Employing dyes in different industrial sectors has produced a serious threat to the environment and living organisms of water bodies and land.					
35728638	13	15	contain	possesses	1811:1819	arg1	CH-ZnS					1804:1809	the CH-ZnS	1800:1809	the CH-ZnS	1800:1809	Results showed that the CH-ZnS possesses excellent efficiency in decontaminating organic dyes from industrial wastewater.					
35728638	13	15	contain	possesses	1811:1819	arg2	efficiency					1831:1840	excellent efficiency	1821:1840	excellent efficiency	1821:1840	Results showed that the CH-ZnS possesses excellent efficiency in decontaminating organic dyes from industrial wastewater.					
35728638	5	16	theme	crystallite	786:796	arg1	size					798:801	a crystallite size	784:801	a crystallite size of 12 nm	784:810	XRD indicated the hexagonal crystal structure of the CS-ZnSe with a crystallite size of 12 nm.					
35728638	1	17	theme	different	178:186	arg1	sectors					199:205	different industrial sectors	178:205	different industrial sectors	178:205	Employing dyes in different industrial sectors has produced a serious threat to the environment and living organisms of water bodies and land.					
35728638	9	18	theme	optimized	1185:1193	arg1	conditions					1195:1204	optimized conditions	1185:1204	optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g)	1185:1291	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	11	19	theme	yellow	1647:1652	arg1	dyes					1654:1657	sunset yellow dyes	1640:1657	sunset yellow dyes	1640:1657	While value for (correlation coefficient, R2) was 0.99307 and 0.99277 for tartrazine and sunset yellow dyes, respectively.					
35728638	9	20	theme	%	1103:1103	arg1	efficiency					1125:1134	a 98% and 97% degradation efficiency	1099:1134	a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye	1099:1180	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	0	21	theme	tartrazine	125:134	arg1	dyes					154:157	tartrazine and sunset yellow dyes	125:157	tartrazine and sunset yellow dyes	125:157	Designing, characterization, and evaluation of chitosan-zinc selenide nanoparticles for visible-light-induced degradation of tartrazine and sunset yellow dyes.					
35728638	7	22	theme	CS-ZnSe	954:960	arg1	composition					935:945	the elemental composition	921:945	the elemental composition of the CS-ZnSe	921:960	EDX analysis was employed to determine the elemental composition of the CS-ZnSe.					
35728638	9	23	theme	97	1109:1110	arg1	%					1111:1111	%	1111:1111	%	1111:1111	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	0	24	theme	Designing	0:8	arg1	characterization					11:26	characterization	11:26	characterization	11:26	Designing, characterization, and evaluation of chitosan-zinc selenide nanoparticles for visible-light-induced degradation of tartrazine and sunset yellow dyes.					
35728638	10	25	theme	Rate	1434:1437	arg1	value					1453:1457	'K' value	1449:1457	'K' value	1449:1457	Rate constant, 'K' value was found to be 0.001362 min-1 and 0.001257 min-1 for tartrazine and SY dyes, respectively.					
35728638	10	25	theme	Rate	1434:1437	arg1	constant					1439:1446	Rate constant	1434:1446	Rate constant	1434:1446	Rate constant, 'K' value was found to be 0.001362 min-1 and 0.001257 min-1 for tartrazine and SY dyes, respectively.					
35728638	9	26	theme	degradation	1113:1123	arg1	efficiency					1125:1134	a 98% and 97% degradation efficiency	1099:1134	a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye	1099:1180	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	2	27	theme	low	409:411	arg1	processes					425:433	an efficient, feasible, and low maintenance processes	381:433	an efficient, feasible, and low maintenance processes	381:433	For the decontamination of such toxic dyes, efforts have been made to develop an efficient, feasible, and low maintenance processes.					
35728638	9	28	theme	process	1369:1375	arg1	Findings					1326:1333	35 °C. Findings	1319:1333	35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes	1319:1431	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	11	29	dep	value	1557:1561	arg1	R2					1593:1594	R2	1593:1594	R2	1593:1594	While value for (correlation coefficient, R2) was 0.99307 and 0.99277 for tartrazine and sunset yellow dyes, respectively.					
35728638	11	29	dep	value	1557:1561	arg1	coefficient					1580:1590	correlation coefficient	1568:1590	correlation coefficient	1568:1590	While value for (correlation coefficient, R2) was 0.99307 and 0.99277 for tartrazine and sunset yellow dyes, respectively.					
35728638	12	30	theme	organic	1766:1772	arg1	dyes					1774:1777	organic dyes	1766:1777	organic dyes	1766:1777	Recyclability of the photocatalyst was confirmed using it for consecutive cycles to degrade organic dyes.					
35728638	10	31	theme	K	1450:1450	arg1	value					1453:1457	'K' value	1449:1457	'K' value	1449:1457	Rate constant, 'K' value was found to be 0.001362 min-1 and 0.001257 min-1 for tartrazine and SY dyes, respectively.					
35728638	10	31	theme	K	1450:1450	arg1	constant					1439:1446	Rate constant	1434:1446	Rate constant	1434:1446	Rate constant, 'K' value was found to be 0.001362 min-1 and 0.001257 min-1 for tartrazine and SY dyes, respectively.					
35728638	0	32	theme	selenide	61:68	arg1	characterization					11:26	characterization	11:26	characterization	11:26	Designing, characterization, and evaluation of chitosan-zinc selenide nanoparticles for visible-light-induced degradation of tartrazine and sunset yellow dyes.					
35728638	0	32	theme	selenide	61:68	arg1	evaluation					33:42	evaluation	33:42	evaluation	33:42	Designing, characterization, and evaluation of chitosan-zinc selenide nanoparticles for visible-light-induced degradation of tartrazine and sunset yellow dyes.					
35728638	3	33	theme	selenide	467:474	arg1	photocatalysts					565:578	the efficient photocatalysts	551:578	the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis	551:639	In this context, chitosan-zinc selenide (CS-ZnSe) nanoparticles were prepared through chemical reduction method as the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis.					
35728638	3	33	theme	selenide	467:474	arg1	nanoparticles					486:498	chitosan-zinc selenide (CS-ZnSe) nanoparticles	453:498	chitosan-zinc selenide (CS-ZnSe) nanoparticles	453:498	In this context, chitosan-zinc selenide (CS-ZnSe) nanoparticles were prepared through chemical reduction method as the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis.					
35728638	9	34	theme	sunset	1159:1164	arg1	dye					1178:1180	sunset yellow (SY) dye	1159:1180	sunset yellow (SY) dye	1159:1180	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	2	35	theme	dyes	341:344	arg1	decontamination					311:325	the decontamination	307:325	the decontamination of such toxic dyes	307:344	For the decontamination of such toxic dyes, efforts have been made to develop an efficient, feasible, and low maintenance processes.					
35728638	11	36	dep	coefficient	1580:1590	arg1	for					1563:1565	for	1563:1565	for	1563:1565	While value for (correlation coefficient, R2) was 0.99307 and 0.99277 for tartrazine and sunset yellow dyes, respectively.					
35728638	12	37	theme	consecutive	1736:1746	arg1	cycles					1748:1753	consecutive cycles	1736:1753	consecutive cycles to degrade organic dyes	1736:1777	Recyclability of the photocatalyst was confirmed using it for consecutive cycles to degrade organic dyes.					
35728638	6	38	theme	nano	848:851	arg1	size					867:870	the average nano photocatalyst size	836:870	the average nano photocatalyst size as 25 nm	836:879	SEM micrographs showed the average nano photocatalyst size as 25 nm.					
35728638	4	39	dep	the	687:689	arg1	help					691:694	help	691:694	help	691:694	Photocatalyst's synthesis was confirmed with the help of FTIR spectroscopy.					
35728638	8	40	theme	excellent	966:974	arg1	efficiency					1003:1012	An excellent photocatalytic degradation efficiency	963:1012	An excellent photocatalytic degradation efficiency for tartrazine and sunset yellow dyes	963:1050	An excellent photocatalytic degradation efficiency for tartrazine and sunset yellow dyes was obtained using CS-ZnSe.					
35728638	12	41	dep	cycles	1748:1753	arg1	degrade					1758:1764	degrade	1758:1764	to degrade organic dyes	1755:1777	Recyclability of the photocatalyst was confirmed using it for consecutive cycles to degrade organic dyes.					
35728638	9	42	theme	35 °C.	1319:1324	arg1	Findings					1326:1333	35 °C. Findings	1319:1333	35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes	1319:1431	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	0	43	theme	chitosan-zinc	47:59	arg1	selenide					61:68	chitosan-zinc selenide	47:68	chitosan-zinc selenide	47:68	Designing, characterization, and evaluation of chitosan-zinc selenide nanoparticles for visible-light-induced degradation of tartrazine and sunset yellow dyes.					
35728638	8	44	theme	degradation	991:1001	arg1	efficiency					1003:1012	An excellent photocatalytic degradation efficiency	963:1012	An excellent photocatalytic degradation efficiency for tartrazine and sunset yellow dyes	963:1050	An excellent photocatalytic degradation efficiency for tartrazine and sunset yellow dyes was obtained using CS-ZnSe.					
35728638	9	45	theme	photocatalytic	1342:1355	arg1	process					1369:1375	the photocatalytic degradation process	1338:1375	the photocatalytic degradation process fitted well with first-order kinetics for both the dyes	1338:1431	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	11	46	theme	correlation	1568:1578	arg1	R2					1593:1594	R2	1593:1594	R2	1593:1594	While value for (correlation coefficient, R2) was 0.99307 and 0.99277 for tartrazine and sunset yellow dyes, respectively.					
35728638	11	46	theme	correlation	1568:1578	arg1	coefficient					1580:1590	correlation coefficient	1568:1590	correlation coefficient	1568:1590	While value for (correlation coefficient, R2) was 0.99307 and 0.99277 for tartrazine and sunset yellow dyes, respectively.					
35728638	6	47	theme	SEM	813:815	arg1	micrographs					817:827	SEM micrographs	813:827	SEM micrographs	813:827	SEM micrographs showed the average nano photocatalyst size as 25 nm.					
35728638	3	48	theme	chemical	522:529	arg1	reduction					531:539	chemical reduction	522:539	chemical reduction method	522:546	In this context, chitosan-zinc selenide (CS-ZnSe) nanoparticles were prepared through chemical reduction method as the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis.					
35728638	7	49	theme	EDX	882:884	arg1	analysis					886:893	EDX analysis	882:893	EDX analysis	882:893	EDX analysis was employed to determine the elemental composition of the CS-ZnSe.					
35728638	1	50	theme	industrial	188:197	arg1	sectors					199:205	different industrial sectors	178:205	different industrial sectors	178:205	Employing dyes in different industrial sectors has produced a serious threat to the environment and living organisms of water bodies and land.					
35728638	8	51	theme	yellow	1040:1045	arg1	dyes					1047:1050	sunset yellow dyes	1033:1050	sunset yellow dyes	1033:1050	An excellent photocatalytic degradation efficiency for tartrazine and sunset yellow dyes was obtained using CS-ZnSe.					
35728638	9	52	theme	time	1209:1212	arg1	conditions					1195:1204	optimized conditions	1185:1204	optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g)	1185:1291	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	2	53	theme	such	330:333	arg1	dyes					341:344	such toxic dyes	330:344	such toxic dyes	330:344	For the decontamination of such toxic dyes, efforts have been made to develop an efficient, feasible, and low maintenance processes.					
35728638	0	54	theme	yellow	147:152	arg1	dyes					154:157	tartrazine and sunset yellow dyes	125:157	tartrazine and sunset yellow dyes	125:157	Designing, characterization, and evaluation of chitosan-zinc selenide nanoparticles for visible-light-induced degradation of tartrazine and sunset yellow dyes.					
35728638	13	55	theme	organic	1861:1867	arg1	dyes					1869:1872	organic dyes	1861:1872	organic dyes	1861:1872	Results showed that the CH-ZnS possesses excellent efficiency in decontaminating organic dyes from industrial wastewater.					
35728638	9	56	theme	first-order	1394:1404	arg1	kinetics					1406:1413	first-order kinetics	1394:1413	first-order kinetics	1394:1413	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	5	57	with	structure	754:762	arg1	size					798:801	a crystallite size	784:801	a crystallite size of 12 nm	784:810	XRD indicated the hexagonal crystal structure of the CS-ZnSe with a crystallite size of 12 nm.					
35728638	11	58	theme	sunset	1640:1645	arg1	dyes					1654:1657	sunset yellow dyes	1640:1657	sunset yellow dyes	1640:1657	While value for (correlation coefficient, R2) was 0.99307 and 0.99277 for tartrazine and sunset yellow dyes, respectively.					
35728638	5	59	theme	CS-ZnSe	771:777	arg1	structure					754:762	the hexagonal crystal structure	732:762	the hexagonal crystal structure of the CS-ZnSe with a crystallite size of 12 nm	732:810	XRD indicated the hexagonal crystal structure of the CS-ZnSe with a crystallite size of 12 nm.					
35728638	9	60	theme	dye	1229:1231	arg1	30 ppm					1248:1253	30 ppm	1248:1253	30 ppm	1248:1253	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	9	60	theme	dye	1229:1231	arg1	time					1209:1212	time (3 h)	1209:1218	time (3 h)	1209:1218	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	9	60	theme	dye	1229:1231	arg1	concentration					1233:1245	dye concentration	1229:1245	dye concentration (30 ppm)	1229:1254	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	3	61	theme	dyes	613:616	arg1	decontamination					588:602	the decontamination	584:602	the decontamination of toxic dyes through photocatalysis	584:639	In this context, chitosan-zinc selenide (CS-ZnSe) nanoparticles were prepared through chemical reduction method as the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis.					
35728638	0	62	theme	visible-light-induced	88:108	arg1	degradation					110:120	visible-light-induced degradation	88:120	visible-light-induced degradation of tartrazine and sunset yellow dyes	88:157	Designing, characterization, and evaluation of chitosan-zinc selenide nanoparticles for visible-light-induced degradation of tartrazine and sunset yellow dyes.					
35728638	5	63	theme	crystal	746:752	arg1	structure					754:762	the hexagonal crystal structure	732:762	the hexagonal crystal structure of the CS-ZnSe with a crystallite size of 12 nm	732:810	XRD indicated the hexagonal crystal structure of the CS-ZnSe with a crystallite size of 12 nm.					
35728638	9	64	theme	98	1101:1102	arg1	%					1103:1103	%	1103:1103	%	1103:1103	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	9	65	theme	catalyst	1257:1264	arg1	0.01 g					1285:1290	0.01 g	1285:1290	0.01 g	1285:1290	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	9	65	theme	catalyst	1257:1264	arg1	time					1209:1212	time (3 h)	1209:1218	time (3 h)	1209:1218	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	9	65	theme	catalyst	1257:1264	arg1	0.09 g					1274:1279	0.09 g	1274:1279	0.09 g	1274:1279	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	9	65	theme	catalyst	1257:1264	arg1	dosage					1266:1271	catalyst dosage	1257:1271	catalyst dosage (0.09 g and 0.01 g)	1257:1291	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	1	66	from	dyes	170:173	arg1	sectors					199:205	different industrial sectors	178:205	different industrial sectors	178:205	Employing dyes in different industrial sectors has produced a serious threat to the environment and living organisms of water bodies and land.					
35728638	9	67	theme	degradation	1357:1367	arg1	process					1369:1375	the photocatalytic degradation process	1338:1375	the photocatalytic degradation process fitted well with first-order kinetics for both the dyes	1338:1431	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	9	68	dep	dyes	1428:1431	arg1	both					1419:1422	both	1419:1422	both	1419:1422	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	2	69	theme	efficient	384:392	arg1	processes					425:433	an efficient, feasible, and low maintenance processes	381:433	an efficient, feasible, and low maintenance processes	381:433	For the decontamination of such toxic dyes, efforts have been made to develop an efficient, feasible, and low maintenance processes.					
35728638	9	70	theme	%	1111:1111	arg1	efficiency					1125:1134	a 98% and 97% degradation efficiency	1099:1134	a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye	1099:1180	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	1	71	theme	land	297:300	arg1	environment					244:254	environment	244:254	environment	244:254	Employing dyes in different industrial sectors has produced a serious threat to the environment and living organisms of water bodies and land.					
35728638	1	71	theme	land	297:300	arg1	organisms					267:275	living organisms	260:275	living organisms	260:275	Employing dyes in different industrial sectors has produced a serious threat to the environment and living organisms of water bodies and land.					
35728638	2	72	theme	maintenance	413:423	arg1	processes					425:433	an efficient, feasible, and low maintenance processes	381:433	an efficient, feasible, and low maintenance processes	381:433	For the decontamination of such toxic dyes, efforts have been made to develop an efficient, feasible, and low maintenance processes.					
35728638	3	73	theme	chitosan-zinc	453:465	arg1	CS-ZnSe					477:483	CS-ZnSe	477:483	CS-ZnSe	477:483	In this context, chitosan-zinc selenide (CS-ZnSe) nanoparticles were prepared through chemical reduction method as the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis.					
35728638	3	73	theme	chitosan-zinc	453:465	arg1	selenide					467:474	chitosan-zinc selenide	453:474	chitosan-zinc selenide (CS-ZnSe) nanoparticles	453:498	In this context, chitosan-zinc selenide (CS-ZnSe) nanoparticles were prepared through chemical reduction method as the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis.					
35728638	9	74	theme	tartrazine	1140:1149	arg1	dye					1151:1153	tartrazine dye	1140:1153	tartrazine dye	1140:1153	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	2	75	theme	feasible	395:402	arg1	processes					425:433	an efficient, feasible, and low maintenance processes	381:433	an efficient, feasible, and low maintenance processes	381:433	For the decontamination of such toxic dyes, efforts have been made to develop an efficient, feasible, and low maintenance processes.					
35728638	5	76	theme	12 nm	806:810	arg1	size					798:801	a crystallite size	784:801	a crystallite size of 12 nm	784:810	XRD indicated the hexagonal crystal structure of the CS-ZnSe with a crystallite size of 12 nm.					
35728638	12	77	theme	photocatalyst	1695:1707	arg1	Recyclability					1674:1686	Recyclability	1674:1686	Recyclability of the photocatalyst	1674:1707	Recyclability of the photocatalyst was confirmed using it for consecutive cycles to degrade organic dyes.					
35728638	5	78	theme	hexagonal	736:744	arg1	structure					754:762	the hexagonal crystal structure	732:762	the hexagonal crystal structure of the CS-ZnSe with a crystallite size of 12 nm	732:810	XRD indicated the hexagonal crystal structure of the CS-ZnSe with a crystallite size of 12 nm.					
35728638	7	79	theme	elemental	925:933	arg1	composition					935:945	the elemental composition	921:945	the elemental composition of the CS-ZnSe	921:960	EDX analysis was employed to determine the elemental composition of the CS-ZnSe.					
35728638	6	80	theme	photocatalyst	853:865	arg1	size					867:870	the average nano photocatalyst size	836:870	the average nano photocatalyst size as 25 nm	836:879	SEM micrographs showed the average nano photocatalyst size as 25 nm.					
35728638	9	81	theme	yellow	1166:1171	arg1	dye					1178:1180	sunset yellow (SY) dye	1159:1180	sunset yellow (SY) dye	1159:1180	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	2	82	theme	toxic	335:339	arg1	dyes					341:344	such toxic dyes	330:344	such toxic dyes	330:344	For the decontamination of such toxic dyes, efforts have been made to develop an efficient, feasible, and low maintenance processes.					
35728638	6	83	theme	average	840:846	arg1	size					867:870	the average nano photocatalyst size	836:870	the average nano photocatalyst size as 25 nm	836:879	SEM micrographs showed the average nano photocatalyst size as 25 nm.					
35728638	1	84	theme	living	260:265	arg1	organisms					267:275	living organisms	260:275	living organisms	260:275	Employing dyes in different industrial sectors has produced a serious threat to the environment and living organisms of water bodies and land.					
35728638	9	85	theme	SY	1174:1175	arg1	dye					1178:1180	sunset yellow (SY) dye	1159:1180	sunset yellow (SY) dye	1159:1180	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35728638	9	86	theme	Findings	1326:1333	arg1	temperature					1304:1314	a temperature	1302:1314	a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes	1302:1431	The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes.					
35042201	6	0	theme	sensor	1050:1055	arg1	selectivity					1022:1032	the selectivity	1018:1032	the selectivity of a particular sensor towards a certain VOC	1018:1077	Each of these fillers enhanced the selectivity of a particular sensor towards a certain VOC compared to the pristine polystyrene-modified sensor.					
35042201	1	1	theme	point-of-care	142:154	arg1	devices					156:162	non-invasive point-of-care devices	129:162	non-invasive point-of-care devices	129:162	Breathomics is the future of non-invasive point-of-care devices.					
35042201	0	2	theme	trace	13:17	arg1	compounds					36:44	trace volatile organic compounds	13:44	trace volatile organic compounds	13:44	Detection of trace volatile organic compounds in spiked breath samples: a leap towards breathomics.					
35042201	8	3	with	interaction	1315:1325	arg1	VOCs					1359:1362	VOCs	1359:1362	VOCs	1359:1362	The interaction of functionalized polymers with VOCs was analyzed by FTIR and UV-vis spectroscopy.					
35042201	3	4	theme	present	304:310	arg1	work					312:315	the present work	300:315	the present work	300:315	In the present work, an array of five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive has been utilized.					
35042201	1	5	theme	devices	156:162	arg1	Breathomics					100:110	Breathomics	100:110	Breathomics	100:110	Breathomics is the future of non-invasive point-of-care devices.					
35042201	1	5	theme	devices	156:162	arg1	future					119:124	future	119:124	future	119:124	Breathomics is the future of non-invasive point-of-care devices.					
35042201	0	6	from	Detection	0:8	arg1	samples					63:69	spiked breath samples	49:69	spiked breath samples	49:69	Detection of trace volatile organic compounds in spiked breath samples: a leap towards breathomics.					
35042201	5	7	theme	dioxide	880:886	arg1	nanoparticles					888:900	titanium dioxide nanoparticles	871:900	titanium dioxide nanoparticles	871:900	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	5	8	theme	nanoparticles-reduced	916:936	arg1	composite					953:961	zinc ferrite nanoparticles-reduced graphene oxide composite	903:961	zinc ferrite nanoparticles-reduced graphene oxide composite	903:961	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	4	9	theme	VOCs	528:531	arg1	ppm					515:517	∼0.5 ppm	510:517	∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol	510:670	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	9	10	theme	%	1520:1520	arg1	accuracy					1522:1529	92.8% accuracy	1516:1529	92.8% accuracy of prediction	1516:1543	The collective sensor response of five sensors is used to identify VOCs using an ensemble classifier with 92.8% accuracy of prediction.					
35042201	5	11	theme	ferrite	908:914	arg1	composite					953:961	zinc ferrite nanoparticles-reduced graphene oxide composite	903:961	zinc ferrite nanoparticles-reduced graphene oxide composite	903:961	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	4	12	theme	known	522:526	arg1	acetaldehyde					550:561	acetaldehyde	550:561	acetaldehyde	550:561	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	12	theme	known	522:526	arg1	VOCs					528:531	known VOCs	522:531	known VOCs	522:531	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	12	theme	known	522:526	arg1	cyclohexanone					623:635	cyclohexanone	623:635	cyclohexanone	623:635	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	12	theme	known	522:526	arg1	alcohol					664:670	isopropyl alcohol	654:670	isopropyl alcohol	654:670	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	12	theme	known	522:526	arg1	octane					564:569	octane	564:569	octane	564:569	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	12	theme	known	522:526	arg1	methanol					589:596	methanol	589:596	methanol	589:596	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	12	theme	known	522:526	arg1	styrene					599:605	styrene	599:605	styrene	599:605	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	12	theme	known	522:526	arg1	butanediol					638:647	butanediol	638:647	butanediol	638:647	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	12	theme	known	522:526	arg1	acetone					541:547	acetone	541:547	acetone	541:547	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	12	theme	known	522:526	arg1	decane					572:577	decane	572:577	decane	572:577	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	12	theme	known	522:526	arg1	ethanol					580:586	ethanol	580:586	ethanol	580:586	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	12	theme	known	522:526	arg1	propylbenzene					608:620	propylbenzene	608:620	propylbenzene	608:620	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	8	13	theme	polymers	1345:1352	arg1	interaction					1315:1325	The interaction	1311:1325	The interaction of functionalized polymers with VOCs	1311:1362	The interaction of functionalized polymers with VOCs was analyzed by FTIR and UV-vis spectroscopy.					
35042201	4	14	used	used	459:462	arg2	array					444:448	The array	440:448	The array	440:448	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	6	15	theme	polystyrene-modified	1104:1123	arg1	sensor					1125:1130	the pristine polystyrene-modified sensor	1091:1130	the pristine polystyrene-modified sensor	1091:1130	Each of these fillers enhanced the selectivity of a particular sensor towards a certain VOC compared to the pristine polystyrene-modified sensor.					
35042201	9	16	theme	ensemble	1491:1498	arg1	classifier					1500:1509	an ensemble classifier	1488:1509	an ensemble classifier with 92.8% accuracy of prediction	1488:1543	The collective sensor response of five sensors is used to identify VOCs using an ensemble classifier with 92.8% accuracy of prediction.					
35042201	10	17	theme	isopropyl	1594:1602	arg1	class					1658:1662	one class	1654:1662	one class	1654:1662	The accuracy of prediction improved to 96% when isopropyl alcohol, ethanol, and methanol were considered as one class.					
35042201	10	17	theme	isopropyl	1594:1602	arg1	methanol					1626:1633	methanol	1626:1633	methanol	1626:1633	The accuracy of prediction improved to 96% when isopropyl alcohol, ethanol, and methanol were considered as one class.					
35042201	10	17	theme	isopropyl	1594:1602	arg1	alcohol					1604:1610	isopropyl alcohol	1594:1610	isopropyl alcohol	1594:1610	The accuracy of prediction improved to 96% when isopropyl alcohol, ethanol, and methanol were considered as one class.					
35042201	10	17	theme	isopropyl	1594:1602	arg1	ethanol					1613:1619	ethanol	1613:1619	ethanol	1613:1619	The accuracy of prediction improved to 96% when isopropyl alcohol, ethanol, and methanol were considered as one class.					
35042201	3	18	theme	-based	359:364	arg1	sensors					366:372	five quartz tuning fork (QTF)-based sensors	330:372	five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive	330:419	In the present work, an array of five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive has been utilized.					
35042201	5	19	theme	zinc	903:906	arg1	composite					953:961	zinc ferrite nanoparticles-reduced graphene oxide composite	903:961	zinc ferrite nanoparticles-reduced graphene oxide composite	903:961	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	3	20	theme	fork	349:352	arg1	sensors					366:372	five quartz tuning fork (QTF)-based sensors	330:372	five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive	330:419	In the present work, an array of five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive has been utilized.					
35042201	3	21	theme	quartz	335:340	arg1	sensors					366:372	five quartz tuning fork (QTF)-based sensors	330:372	five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive	330:419	In the present work, an array of five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive has been utilized.					
35042201	5	22	theme	graphene	938:945	arg1	composite					953:961	zinc ferrite nanoparticles-reduced graphene oxide composite	903:961	zinc ferrite nanoparticles-reduced graphene oxide composite	903:961	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	5	23	theme	oxide	854:858	arg1	composite					860:868	silver nanoparticles-reduced graphene oxide composite	816:868	silver nanoparticles-reduced graphene oxide composite	816:868	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	9	24	with	classifier	1500:1509	arg1	accuracy					1522:1529	92.8% accuracy	1516:1529	92.8% accuracy of prediction	1516:1543	The collective sensor response of five sensors is used to identify VOCs using an ensemble classifier with 92.8% accuracy of prediction.					
35042201	7	25	from	change	1229:1234	arg1	frequency					1252:1260	the resonant frequency	1239:1260	the resonant frequency of QTF	1239:1267	Their interaction with the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF is used as sensor response for detection.					
35042201	7	26	theme	QTF	1265:1267	arg1	frequency					1252:1260	the resonant frequency	1239:1260	the resonant frequency of QTF	1239:1267	Their interaction with the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF is used as sensor response for detection.					
35042201	0	27	theme	volatile	19:26	arg1	compounds					36:44	trace volatile organic compounds	13:44	trace volatile organic compounds	13:44	Detection of trace volatile organic compounds in spiked breath samples: a leap towards breathomics.					
35042201	4	28	theme	breath	491:496	arg1	samples					474:480	samples	474:480	samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases	474:713	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	28	theme	breath	491:496	arg1	bio-markers					682:692	bio-markers	682:692	bio-markers for certain diseases	682:713	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	8	29	theme	UV-vis	1389:1394	arg1	spectroscopy					1396:1407	FTIR and UV-vis spectroscopy	1380:1407	FTIR and UV-vis spectroscopy	1380:1407	The interaction of functionalized polymers with VOCs was analyzed by FTIR and UV-vis spectroscopy.					
35042201	7	30	theme	sensor	1280:1285	arg1	response					1287:1294	sensor response	1280:1294	sensor response for detection	1280:1308	Their interaction with the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF is used as sensor response for detection.					
35042201	7	30	theme	sensor	1280:1285	arg1	interaction					1139:1149	Their interaction	1133:1149	Their interaction with the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF	1133:1267	Their interaction with the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF is used as sensor response for detection.					
35042201	2	31	theme	detection	286:294	arg1	isolation					212:220	the isolation	208:220	the isolation of disease-specific volatile organic compounds (VOCs) and their detection	208:294	The field of breathomics can be split into the isolation of disease-specific volatile organic compounds (VOCs) and their detection.					
35042201	7	32	theme	resonant	1243:1250	arg1	frequency					1252:1260	the resonant frequency	1239:1260	the resonant frequency of QTF	1239:1267	Their interaction with the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF is used as sensor response for detection.					
35042201	3	33	theme	tuning	342:347	arg1	sensors					366:372	five quartz tuning fork (QTF)-based sensors	330:372	five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive	330:419	In the present work, an array of five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive has been utilized.					
35042201	7	34	used	used	1272:1275	arg2	interaction					1139:1149	Their interaction	1133:1149	Their interaction with the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF	1133:1267	Their interaction with the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF is used as sensor response for detection.					
35042201	7	34	used	used	1272:1275	arg2	response					1287:1294	sensor response	1280:1294	sensor response for detection	1280:1308	Their interaction with the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF is used as sensor response for detection.					
35042201	5	35	theme	titanium	871:878	arg1	dioxide					880:886	titanium dioxide	871:886	titanium dioxide nanoparticles	871:900	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	5	36	theme	base	744:747	arg1	polymer					749:755	the base polymer	740:755	the base polymer	740:755	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	5	36	theme	base	744:747	arg1	Polystyrene					716:726	Polystyrene	716:726	Polystyrene	716:726	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	0	37	theme	compounds	36:44	arg1	Detection					0:8	Detection	0:8	Detection of trace volatile organic compounds in spiked breath samples: a	0:72	Detection of trace volatile organic compounds in spiked breath samples: a leap towards breathomics.					
35042201	5	38	theme	oxide	947:951	arg1	composite					953:961	zinc ferrite nanoparticles-reduced graphene oxide composite	903:961	zinc ferrite nanoparticles-reduced graphene oxide composite	903:961	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	8	39	theme	FTIR	1380:1383	arg1	spectroscopy					1396:1407	FTIR and UV-vis spectroscopy	1380:1407	FTIR and UV-vis spectroscopy	1380:1407	The interaction of functionalized polymers with VOCs was analyzed by FTIR and UV-vis spectroscopy.					
35042201	8	40	theme	functionalized	1330:1343	arg1	polymers					1345:1352	functionalized polymers	1330:1352	functionalized polymers	1330:1352	The interaction of functionalized polymers with VOCs was analyzed by FTIR and UV-vis spectroscopy.					
35042201	9	41	theme	collective	1414:1423	arg1	response					1432:1439	The collective sensor response	1410:1439	The collective sensor response of five sensors	1410:1455	The collective sensor response of five sensors is used to identify VOCs using an ensemble classifier with 92.8% accuracy of prediction.					
35042201	0	42	theme	organic	28:34	arg1	compounds					36:44	trace volatile organic compounds	13:44	trace volatile organic compounds	13:44	Detection of trace volatile organic compounds in spiked breath samples: a leap towards breathomics.					
35042201	3	43	theme	sensors	366:372	arg1	array					321:325	an array	318:325	an array of five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive	318:419	In the present work, an array of five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive has been utilized.					
35042201	7	44	with	interaction	1139:1149	arg1	VOCs					1160:1163	the VOCs	1156:1163	the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF	1156:1267	Their interaction with the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF is used as sensor response for detection.					
35042201	3	45	used	utilized	430:437	arg2	array					321:325	an array	318:325	an array of five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive	318:419	In the present work, an array of five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive has been utilized.					
35042201	9	46	theme	92.8	1516:1519	arg1	%					1520:1520	%	1520:1520	%	1520:1520	The collective sensor response of five sensors is used to identify VOCs using an ensemble classifier with 92.8% accuracy of prediction.					
35042201	3	47	theme	QTF	355:357	arg1	sensors					366:372	five quartz tuning fork (QTF)-based sensors	330:372	five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive	330:419	In the present work, an array of five quartz tuning fork (QTF)-based sensors modified by polymer with nanomaterial additive has been utilized.					
35042201	0	48	theme	breath	56:61	arg1	samples					63:69	spiked breath samples	49:69	spiked breath samples	49:69	Detection of trace volatile organic compounds in spiked breath samples: a leap towards breathomics.					
35042201	9	49	theme	prediction	1534:1543	arg1	accuracy					1522:1529	92.8% accuracy	1516:1529	92.8% accuracy of prediction	1516:1543	The collective sensor response of five sensors is used to identify VOCs using an ensemble classifier with 92.8% accuracy of prediction.					
35042201	6	50	theme	particular	1039:1048	arg1	sensor					1050:1055	a particular sensor	1037:1055	a particular sensor	1037:1055	Each of these fillers enhanced the selectivity of a particular sensor towards a certain VOC compared to the pristine polystyrene-modified sensor.					
35042201	6	51	theme	certain	1067:1073	arg1	VOC					1075:1077	a certain VOC	1065:1077	a certain VOC	1065:1077	Each of these fillers enhanced the selectivity of a particular sensor towards a certain VOC compared to the pristine polystyrene-modified sensor.					
35042201	9	52	theme	sensors	1449:1455	arg1	response					1432:1439	The collective sensor response	1410:1439	The collective sensor response of five sensors	1410:1455	The collective sensor response of five sensors is used to identify VOCs using an ensemble classifier with 92.8% accuracy of prediction.					
35042201	4	53	theme	human	485:489	arg1	breath					491:496	human breath	485:496	human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol	485:670	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	5	54	used	used	732:735	arg2	Polystyrene					716:726	Polystyrene	716:726	Polystyrene	716:726	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	5	54	used	used	732:735	arg2	polymer					749:755	the base polymer	740:755	the base polymer	740:755	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	5	55	theme	cellulose	968:976	arg1	acetate					978:984	cellulose acetate	968:984	cellulose acetate	968:984	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	5	56	theme	silver	816:821	arg1	composite					860:868	silver nanoparticles-reduced graphene oxide composite	816:868	silver nanoparticles-reduced graphene oxide composite	816:868	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	0	57	dep	Detection	0:8	arg1	a					72:72	a	72:72	Detection of trace volatile organic compounds in spiked breath samples: a	0:72	Detection of trace volatile organic compounds in spiked breath samples: a leap towards breathomics.					
35042201	10	58	theme	prediction	1562:1571	arg1	accuracy					1550:1557	The accuracy	1546:1557	The accuracy of prediction	1546:1571	The accuracy of prediction improved to 96% when isopropyl alcohol, ethanol, and methanol were considered as one class.					
35042201	4	59	theme	certain	698:704	arg1	diseases					706:713	certain diseases	698:713	certain diseases	698:713	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	2	60	theme	compounds	259:267	arg1	isolation					212:220	the isolation	208:220	the isolation of disease-specific volatile organic compounds (VOCs) and their detection	208:294	The field of breathomics can be split into the isolation of disease-specific volatile organic compounds (VOCs) and their detection.					
35042201	7	61	theme	polymer	1206:1212	arg1	properties					1192:1201	the mechanical properties	1177:1201	the mechanical properties of polymer giving rise to change in the resonant frequency of QTF	1177:1267	Their interaction with the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF is used as sensor response for detection.					
35042201	5	62	theme	nanoparticles-reduced	823:843	arg1	composite					860:868	silver nanoparticles-reduced graphene oxide composite	816:868	silver nanoparticles-reduced graphene oxide composite	816:868	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	4	63	theme	isopropyl	654:662	arg1	VOCs					528:531	known VOCs	522:531	known VOCs	522:531	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	4	63	theme	isopropyl	654:662	arg1	alcohol					664:670	isopropyl alcohol	654:670	isopropyl alcohol	654:670	The array has been used to detect samples of human breath spiked with ∼0.5 ppm of known VOCs namely, acetone, acetaldehyde, octane, decane, ethanol, methanol, styrene, propylbenzene, cyclohexanone, butanediol, and isopropyl alcohol which are bio-markers for certain diseases.					
35042201	6	64	theme	pristine	1095:1102	arg1	sensor					1125:1130	the pristine polystyrene-modified sensor	1091:1130	the pristine polystyrene-modified sensor	1091:1130	Each of these fillers enhanced the selectivity of a particular sensor towards a certain VOC compared to the pristine polystyrene-modified sensor.					
35042201	2	65	theme	organic	251:257	arg1	VOCs					270:273	VOCs	270:273	VOCs	270:273	The field of breathomics can be split into the isolation of disease-specific volatile organic compounds (VOCs) and their detection.					
35042201	2	65	theme	organic	251:257	arg1	compounds					259:267	disease-specific volatile organic compounds	225:267	disease-specific volatile organic compounds (VOCs)	225:274	The field of breathomics can be split into the isolation of disease-specific volatile organic compounds (VOCs) and their detection.					
35042201	5	66	theme	graphene	845:852	arg1	composite					860:868	silver nanoparticles-reduced graphene oxide composite	816:868	silver nanoparticles-reduced graphene oxide composite	816:868	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	9	67	used	used	1460:1463	arg2	response					1432:1439	The collective sensor response	1410:1439	The collective sensor response of five sensors	1410:1455	The collective sensor response of five sensors is used to identify VOCs using an ensemble classifier with 92.8% accuracy of prediction.					
35042201	9	68	theme	sensor	1425:1430	arg1	response					1432:1439	The collective sensor response	1410:1439	The collective sensor response of five sensors	1410:1455	The collective sensor response of five sensors is used to identify VOCs using an ensemble classifier with 92.8% accuracy of prediction.					
35042201	2	69	theme	breathomics	178:188	arg1	field					169:173	The field	165:173	The field of breathomics	165:188	The field of breathomics can be split into the isolation of disease-specific volatile organic compounds (VOCs) and their detection.					
35042201	2	70	theme	volatile	242:249	arg1	VOCs					270:273	VOCs	270:273	VOCs	270:273	The field of breathomics can be split into the isolation of disease-specific volatile organic compounds (VOCs) and their detection.					
35042201	2	70	theme	volatile	242:249	arg1	compounds					259:267	disease-specific volatile organic compounds	225:267	disease-specific volatile organic compounds (VOCs)	225:274	The field of breathomics can be split into the isolation of disease-specific volatile organic compounds (VOCs) and their detection.					
35042201	5	71	theme	different	790:798	arg1	fillers					800:806	4 different fillers	788:806	4 different fillers namely	788:813	Polystyrene was used as the base polymer and it was functionalized with 4 different fillers namely, silver nanoparticles-reduced graphene oxide composite, titanium dioxide nanoparticles, zinc ferrite nanoparticles-reduced graphene oxide composite, and cellulose acetate.					
35042201	0	72	theme	spiked	49:54	arg1	breath					56:61	spiked breath	49:61	spiked breath samples	49:69	Detection of trace volatile organic compounds in spiked breath samples: a leap towards breathomics.					
35042201	2	73	theme	disease-specific	225:240	arg1	VOCs					270:273	VOCs	270:273	VOCs	270:273	The field of breathomics can be split into the isolation of disease-specific volatile organic compounds (VOCs) and their detection.					
35042201	2	73	theme	disease-specific	225:240	arg1	compounds					259:267	disease-specific volatile organic compounds	225:267	disease-specific volatile organic compounds (VOCs)	225:274	The field of breathomics can be split into the isolation of disease-specific volatile organic compounds (VOCs) and their detection.					
35042201	1	74	theme	non-invasive	129:140	arg1	devices					156:162	non-invasive point-of-care devices	129:162	non-invasive point-of-care devices	129:162	Breathomics is the future of non-invasive point-of-care devices.					
35042201	7	75	theme	mechanical	1181:1190	arg1	properties					1192:1201	the mechanical properties	1177:1201	the mechanical properties of polymer giving rise to change in the resonant frequency of QTF	1177:1267	Their interaction with the VOCs in changing the mechanical properties of polymer giving rise to change in the resonant frequency of QTF is used as sensor response for detection.					
35136180	5	0	theme	single	657:662	arg1	domain					681:686	a single membrane-binding domain	655:686	a single membrane-binding domain	655:686	Human Alg2 (hAlg2) associates with the ER via a single membrane-binding domain and is markedly more stable in vitro.					
35136180	0	1	link	lipid-linked	89:100	arg1	oligosaccharide					102:116	lipid-linked oligosaccharide	89:116	lipid-linked oligosaccharide biosynthetic pathway	89:137	Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway.					
35136180	5	2	theme	membrane-binding	664:679	arg1	domain					681:686	a single membrane-binding domain	655:686	a single membrane-binding domain	655:686	Human Alg2 (hAlg2) associates with the ER via a single membrane-binding domain and is markedly more stable in vitro.					
35136180	1	3	theme	lipid-linked	188:199	arg1	LLO					218:220	LLO	218:220	LLO	218:220	N-glycosylation starts with the biosynthesis of lipid-linked oligosaccharide (LLO) on the endoplasmic reticulum (ER).					
35136180	1	3	theme	lipid-linked	188:199	arg1	oligosaccharide					201:215	lipid-linked oligosaccharide	188:215	lipid-linked oligosaccharide (LLO)	188:221	N-glycosylation starts with the biosynthesis of lipid-linked oligosaccharide (LLO) on the endoplasmic reticulum (ER).					
35136180	0	4	theme	oligosaccharide	102:116	arg1	pathway					131:137	lipid-linked oligosaccharide biosynthetic pathway	89:137	lipid-linked oligosaccharide biosynthetic pathway	89:137	Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway.					
35136180	6	5	theme	kinetics	824:831	arg1	assay					833:837	a liquid chromatography-mass spectrometry quantitative kinetics assay	769:837	a liquid chromatography-mass spectrometry quantitative kinetics assay for studying purified hAlg2	769:865	These properties were exploited to develop a liquid chromatography-mass spectrometry quantitative kinetics assay for studying purified hAlg2.					
35136180	8	6	theme	substrate	1065:1073	arg1	level					1050:1054	an increased level	1037:1054	an increased level of M1Gn2 substrate	1037:1073	However, this bias is altered by an excess of GDP-Man donor or an increased level of M1Gn2 substrate, both of which trigger production of the M2Gn2(α-1,6)-PDol.					
35136180	8	6	theme	substrate	1065:1073	arg1	donor					1028:1032	GDP-Man donor	1020:1032	GDP-Man donor	1020:1032	However, this bias is altered by an excess of GDP-Man donor or an increased level of M1Gn2 substrate, both of which trigger production of the M2Gn2(α-1,6)-PDol.					
35136180	6	7	theme	purified	852:859	arg1	hAlg2					861:865	purified hAlg2	852:865	purified hAlg2	852:865	These properties were exploited to develop a liquid chromatography-mass spectrometry quantitative kinetics assay for studying purified hAlg2.					
35136180	0	8	theme	lipid-linked	89:100	arg1	oligosaccharide					102:116	lipid-linked oligosaccharide	89:116	lipid-linked oligosaccharide biosynthetic pathway	89:137	Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway.					
35136180	8	9	theme	M1Gn2	1059:1063	arg1	substrate					1065:1073	M1Gn2 substrate	1059:1073	M1Gn2 substrate	1059:1073	However, this bias is altered by an excess of GDP-Man donor or an increased level of M1Gn2 substrate, both of which trigger production of the M2Gn2(α-1,6)-PDol.					
35136180	8	10	theme	GDP-Man	1020:1026	arg1	donor					1028:1032	GDP-Man donor	1020:1032	GDP-Man donor	1020:1032	However, this bias is altered by an excess of GDP-Man donor or an increased level of M1Gn2 substrate, both of which trigger production of the M2Gn2(α-1,6)-PDol.					
35136180	5	11	from	vitro	719:723	arg1	stable					709:714	stable	709:714	stable	709:714	Human Alg2 (hAlg2) associates with the ER via a single membrane-binding domain and is markedly more stable in vitro.					
35136180	8	12	theme	donor	1028:1032	arg1	excess					1010:1015	an excess	1007:1015	an excess	1007:1015	However, this bias is altered by an excess of GDP-Man donor or an increased level of M1Gn2 substrate, both of which trigger production of the M2Gn2(α-1,6)-PDol.					
35136180	1	13	from	biosynthesis	172:183	arg1	ER					253:254	ER	253:254	ER	253:254	N-glycosylation starts with the biosynthesis of lipid-linked oligosaccharide (LLO) on the endoplasmic reticulum (ER).					
35136180	1	13	from	biosynthesis	172:183	arg1	reticulum					242:250	the endoplasmic reticulum	226:250	the endoplasmic reticulum (ER)	226:255	N-glycosylation starts with the biosynthesis of lipid-linked oligosaccharide (LLO) on the endoplasmic reticulum (ER).					
35136180	1	14	theme	oligosaccharide	201:215	arg1	biosynthesis					172:183	the biosynthesis	168:183	the biosynthesis of lipid-linked oligosaccharide (LLO) on the endoplasmic reticulum (ER)	168:255	N-glycosylation starts with the biosynthesis of lipid-linked oligosaccharide (LLO) on the endoplasmic reticulum (ER).					
35136180	0	15	theme	biosynthetic	118:129	arg1	pathway					131:137	lipid-linked oligosaccharide biosynthetic pathway	89:137	lipid-linked oligosaccharide biosynthetic pathway	89:137	Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway.					
35136180	1	16	theme	endoplasmic	230:240	arg1	ER					253:254	ER	253:254	ER	253:254	N-glycosylation starts with the biosynthesis of lipid-linked oligosaccharide (LLO) on the endoplasmic reticulum (ER).					
35136180	1	16	theme	endoplasmic	230:240	arg1	reticulum					242:250	the endoplasmic reticulum	226:250	the endoplasmic reticulum (ER)	226:255	N-glycosylation starts with the biosynthesis of lipid-linked oligosaccharide (LLO) on the endoplasmic reticulum (ER).					
35136180	2	17	theme	branched	410:417	arg1	product					430:436	the branched M3Gn2-PDol product	406:436	the branched M3Gn2-PDol product	406:436	Alg2 mannosyltransferase adds both the α1,3- and α1,6-mannose (Man) onto ManGlcNAc2-pyrophosphate-dolichol (M1Gn2-PDol) in either order to generate the branched M3Gn2-PDol product.					
35136180	5	18	theme	Human	609:613	arg1	Alg2					615:618	Human Alg2	609:618	Human Alg2 (hAlg2)	609:626	Human Alg2 (hAlg2) associates with the ER via a single membrane-binding domain and is markedly more stable in vitro.					
35136180	5	18	theme	Human	609:613	arg1	hAlg2					621:625	hAlg2	621:625	hAlg2	621:625	Human Alg2 (hAlg2) associates with the ER via a single membrane-binding domain and is markedly more stable in vitro.					
35136180	3	19	theme	yeast	456:460	arg1	Alg2					462:465	The well-studied yeast Alg2	439:465	The well-studied yeast Alg2	439:465	The well-studied yeast Alg2 interacts with ER membrane through four hydrophobic domains.					
35136180	0	20	theme	Topological	0:10	arg1	analysis					26:33	Topological and enzymatic analysis	0:33	Topological and enzymatic analysis of human Alg2 mannosyltransferase	0:67	Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway.					
35136180	6	21	theme	quantitative	811:822	arg1	assay					833:837	a liquid chromatography-mass spectrometry quantitative kinetics assay	769:837	a liquid chromatography-mass spectrometry quantitative kinetics assay for studying purified hAlg2	769:865	These properties were exploited to develop a liquid chromatography-mass spectrometry quantitative kinetics assay for studying purified hAlg2.					
35136180	7	22	theme	physiological	874:886	arg1	conditions					888:897	physiological conditions	874:897	physiological conditions	874:897	Under physiological conditions, hAlg2 prefers to transfer α1,3-Man onto M1Gn2 before adding the α1,6-Man.					
35136180	8	23	theme	level	1050:1054	arg1	excess					1010:1015	an excess	1007:1015	an excess	1007:1015	However, this bias is altered by an excess of GDP-Man donor or an increased level of M1Gn2 substrate, both of which trigger production of the M2Gn2(α-1,6)-PDol.					
35136180	8	24	theme	increased	1040:1048	arg1	level					1050:1054	an increased level	1037:1054	an increased level of M1Gn2 substrate	1037:1073	However, this bias is altered by an excess of GDP-Man donor or an increased level of M1Gn2 substrate, both of which trigger production of the M2Gn2(α-1,6)-PDol.					
35136180	6	25	theme	spectrometry	798:809	arg1	assay					833:837	a liquid chromatography-mass spectrometry quantitative kinetics assay	769:837	a liquid chromatography-mass spectrometry quantitative kinetics assay for studying purified hAlg2	769:865	These properties were exploited to develop a liquid chromatography-mass spectrometry quantitative kinetics assay for studying purified hAlg2.					
35136180	9	26	theme	M2Gn2	1240:1244	arg1	intermediate					1254:1265	M2Gn2 (α-1,6) intermediate	1240:1265	M2Gn2 (α-1,6) intermediate	1240:1265	These results suggest that Alg2 may regulate the LLO biosynthetic pathway by controlling accumulation of M2Gn2 (α-1,6) intermediate.					
35136180	0	27	theme	enzymatic	16:24	arg1	analysis					26:33	Topological and enzymatic analysis	0:33	Topological and enzymatic analysis of human Alg2 mannosyltransferase	0:67	Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway.					
35136180	1	28	link	lipid-linked	188:199	arg1	LLO					218:220	LLO	218:220	LLO	218:220	N-glycosylation starts with the biosynthesis of lipid-linked oligosaccharide (LLO) on the endoplasmic reticulum (ER).					
35136180	1	28	link	lipid-linked	188:199	arg1	oligosaccharide					201:215	lipid-linked oligosaccharide	188:215	lipid-linked oligosaccharide (LLO)	188:221	N-glycosylation starts with the biosynthesis of lipid-linked oligosaccharide (LLO) on the endoplasmic reticulum (ER).					
35136180	0	29	theme	human	38:42	arg1	mannosyltransferase					49:67	human Alg2 mannosyltransferase	38:67	human Alg2 mannosyltransferase	38:67	Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway.					
35136180	0	30	from	role	81:84	arg1	pathway					131:137	lipid-linked oligosaccharide biosynthetic pathway	89:137	lipid-linked oligosaccharide biosynthetic pathway	89:137	Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway.					
35136180	3	31	theme	ER	482:483	arg1	membrane					485:492	ER membrane	482:492	ER membrane	482:492	The well-studied yeast Alg2 interacts with ER membrane through four hydrophobic domains.					
35136180	2	32	theme	M3Gn2-PDol	419:428	arg1	product					430:436	the branched M3Gn2-PDol product	406:436	the branched M3Gn2-PDol product	406:436	Alg2 mannosyltransferase adds both the α1,3- and α1,6-mannose (Man) onto ManGlcNAc2-pyrophosphate-dolichol (M1Gn2-PDol) in either order to generate the branched M3Gn2-PDol product.					
35136180	3	33	theme	well-studied	443:454	arg1	Alg2					462:465	The well-studied yeast Alg2	439:465	The well-studied yeast Alg2	439:465	The well-studied yeast Alg2 interacts with ER membrane through four hydrophobic domains.					
35136180	6	34	theme	chromatography-mass	778:796	arg1	assay					833:837	a liquid chromatography-mass spectrometry quantitative kinetics assay	769:837	a liquid chromatography-mass spectrometry quantitative kinetics assay for studying purified hAlg2	769:865	These properties were exploited to develop a liquid chromatography-mass spectrometry quantitative kinetics assay for studying purified hAlg2.					
35136180	0	35	theme	mannosyltransferase	49:67	arg1	analysis					26:33	Topological and enzymatic analysis	0:33	Topological and enzymatic analysis of human Alg2 mannosyltransferase	0:67	Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway.					
35136180	4	36	theme	Alg2	555:558	arg1	structure					560:568	Alg2 structure	555:568	Alg2 structure	555:568	Unexpectedly, we show that Alg2 structure has diverged between yeast and humans.					
35136180	9	37	theme	LLO	1184:1186	arg1	pathway					1201:1207	the LLO biosynthetic pathway	1180:1207	the LLO biosynthetic pathway	1180:1207	These results suggest that Alg2 may regulate the LLO biosynthetic pathway by controlling accumulation of M2Gn2 (α-1,6) intermediate.					
35136180	9	38	theme	α-1,6	1247:1251	arg1	intermediate					1254:1265	M2Gn2 (α-1,6) intermediate	1240:1265	M2Gn2 (α-1,6) intermediate	1240:1265	These results suggest that Alg2 may regulate the LLO biosynthetic pathway by controlling accumulation of M2Gn2 (α-1,6) intermediate.					
35136180	6	39	theme	liquid	771:776	arg1	assay					833:837	a liquid chromatography-mass spectrometry quantitative kinetics assay	769:837	a liquid chromatography-mass spectrometry quantitative kinetics assay for studying purified hAlg2	769:865	These properties were exploited to develop a liquid chromatography-mass spectrometry quantitative kinetics assay for studying purified hAlg2.					
35136180	0	40	theme	Alg2	44:47	arg1	mannosyltransferase					49:67	human Alg2 mannosyltransferase	38:67	human Alg2 mannosyltransferase	38:67	Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway.					
35136180	8	41	theme	M2Gn2	1116:1120	arg1	-PDol					1128:1132	the M2Gn2(α-1,6)-PDol	1112:1132	the M2Gn2(α-1,6)-PDol	1112:1132	However, this bias is altered by an excess of GDP-Man donor or an increased level of M1Gn2 substrate, both of which trigger production of the M2Gn2(α-1,6)-PDol.					
35136180	9	42	theme	intermediate	1254:1265	arg1	accumulation					1224:1235	accumulation	1224:1235	accumulation of M2Gn2 (α-1,6) intermediate	1224:1265	These results suggest that Alg2 may regulate the LLO biosynthetic pathway by controlling accumulation of M2Gn2 (α-1,6) intermediate.					
35136180	3	43	theme	hydrophobic	507:517	arg1	domains					519:525	four hydrophobic domains	502:525	four hydrophobic domains	502:525	The well-studied yeast Alg2 interacts with ER membrane through four hydrophobic domains.					
35136180	8	44	theme	α-1,6	1122:1126	arg1	-PDol					1128:1132	the M2Gn2(α-1,6)-PDol	1112:1132	the M2Gn2(α-1,6)-PDol	1112:1132	However, this bias is altered by an excess of GDP-Man donor or an increased level of M1Gn2 substrate, both of which trigger production of the M2Gn2(α-1,6)-PDol.					
35136180	9	45	theme	biosynthetic	1188:1199	arg1	pathway					1201:1207	the LLO biosynthetic pathway	1180:1207	the LLO biosynthetic pathway	1180:1207	These results suggest that Alg2 may regulate the LLO biosynthetic pathway by controlling accumulation of M2Gn2 (α-1,6) intermediate.					
35136180	5	46	from	stable	709:714	arg1	vitro					719:723	vitro	719:723	vitro	719:723	Human Alg2 (hAlg2) associates with the ER via a single membrane-binding domain and is markedly more stable in vitro.					
35136180	2	47	dep	α1,3-	297:301	arg1	the					293:295	the	293:295	the	293:295	Alg2 mannosyltransferase adds both the α1,3- and α1,6-mannose (Man) onto ManGlcNAc2-pyrophosphate-dolichol (M1Gn2-PDol) in either order to generate the branched M3Gn2-PDol product.					
35136180	8	48	theme	-PDol	1128:1132	arg1	production					1098:1107	production	1098:1107	production of the M2Gn2(α-1,6)-PDol	1098:1132	However, this bias is altered by an excess of GDP-Man donor or an increased level of M1Gn2 substrate, both of which trigger production of the M2Gn2(α-1,6)-PDol.					
35136180	2	49	theme	Alg2	258:261	arg1	mannosyltransferase					263:281	Alg2 mannosyltransferase	258:281	Alg2 mannosyltransferase	258:281	Alg2 mannosyltransferase adds both the α1,3- and α1,6-mannose (Man) onto ManGlcNAc2-pyrophosphate-dolichol (M1Gn2-PDol) in either order to generate the branched M3Gn2-PDol product.					
36314398	0	0	theme	Robust	71:76	arg1	Dressing					110:117	Robust and High-Performance Hemostatic Dressing	71:117	Robust and High-Performance Hemostatic Dressing	71:117	Assembly of Clay Nanotubes on Cotton Fibers Mediated by Biopolymer for Robust and High-Performance Hemostatic Dressing.					
36314398	5	1	theme	wound	814:818	arg1	area					820:823	the wound area	810:823	the wound area	810:823	The robust clay nanotube dressing materials maintain high procoagulant activity after harsh water treatment, and only a few residuals of halloysite exist in the wound area.					
36314398	6	2	theme	high	991:994	arg1	safety					996:1001	high safety	991:1001	high safety	991:1001	Compared with commercial hemostat QuikClot Combat gauze, halloysite-alginate-cotton composite dressing exhibits hemostatic properties both in vivo and in vitro with high safety.					
36314398	5	3	theme	water	745:749	arg1	treatment					751:759	harsh water treatment	739:759	harsh water treatment	739:759	The robust clay nanotube dressing materials maintain high procoagulant activity after harsh water treatment, and only a few residuals of halloysite exist in the wound area.					
36314398	4	4	theme	cotton	563:568	arg1	fibers					570:575	commercial cotton fibers	552:575	commercial cotton fibers	552:575	Here, an efficient clay hemostat of halloysite nanotubes is tightly bound onto commercial cotton fibers, which is capillary mediated by biopolymer alginate with Ca2+ crosslinking.					
36314398	8	5	theme	hemostatic	1341:1350	arg1	activity					1352:1359	balanced high hemostatic activity	1327:1359	balanced high hemostatic activity	1327:1359	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	4	6	theme	clay	492:495	arg1	hemostat					497:504	an efficient clay hemostat	479:504	an efficient clay hemostat of halloysite nanotubes	479:528	Here, an efficient clay hemostat of halloysite nanotubes is tightly bound onto commercial cotton fibers, which is capillary mediated by biopolymer alginate with Ca2+ crosslinking.					
36314398	7	7	theme	halloysite	1143:1152	arg1	factors					1166:1172	halloysite coagulation factors	1143:1172	halloysite coagulation factors	1143:1172	The hemostatic mechanism of the dressing is attributed to activating platelets, locally concentrating clotting components in the nanoclay, halloysite coagulation factors, and alginate cross-linked with Ca2+ .					
36314398	4	8	theme	commercial	552:561	arg1	fibers					570:575	commercial cotton fibers	552:575	commercial cotton fibers	552:575	Here, an efficient clay hemostat of halloysite nanotubes is tightly bound onto commercial cotton fibers, which is capillary mediated by biopolymer alginate with Ca2+ crosslinking.					
36314398	0	9	theme	High-Performance	82:97	arg1	Dressing					110:117	Robust and High-Performance Hemostatic Dressing	71:117	Robust and High-Performance Hemostatic Dressing	71:117	Assembly of Clay Nanotubes on Cotton Fibers Mediated by Biopolymer for Robust and High-Performance Hemostatic Dressing.					
36314398	0	10	from	Assembly	0:7	arg1	Fibers					37:42	Cotton Fibers	30:42	Cotton Fibers	30:42	Assembly of Clay Nanotubes on Cotton Fibers Mediated by Biopolymer for Robust and High-Performance Hemostatic Dressing.					
36314398	1	11	from	accidents	169:177	arg1	bleeding					135:142	bleeding	135:142	bleeding	135:142	Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor.					
36314398	1	11	from	accidents	169:177	arg1	factor					232:237	a major life-threatening factor	207:237	a major life-threatening factor	207:237	Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor.					
36314398	6	12	theme	halloysite-alginate-cotton	883:908	arg1	dressing					920:927	halloysite-alginate-cotton composite dressing	883:927	halloysite-alginate-cotton composite dressing	883:927	Compared with commercial hemostat QuikClot Combat gauze, halloysite-alginate-cotton composite dressing exhibits hemostatic properties both in vivo and in vitro with high safety.					
36314398	8	13	theme	ingredient	1370:1379	arg1	loss					1381:1384	minimal ingredient loss	1362:1384	minimal ingredient loss	1362:1384	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	2	14	theme	patients	319:326	arg1	survival					298:305	the survival	294:305	the survival of bleeding patients in prehospital care	294:346	Rapid, safe, and convenient hemostasis is critical to the survival of bleeding patients in prehospital care.					
36314398	5	15	theme	harsh	739:743	arg1	treatment					751:759	harsh water treatment	739:759	harsh water treatment	739:759	The robust clay nanotube dressing materials maintain high procoagulant activity after harsh water treatment, and only a few residuals of halloysite exist in the wound area.					
36314398	9	16	theme	health	1553:1558	arg1	risks					1560:1564	low health risks	1549:1564	low health risks	1549:1564	The robust dressing based on halloysite tightly bounded cotton shows great potential for military, medical, and civil bleeding control with low health risks.					
36314398	1	17	from	conflicts	158:166	arg1	bleeding					135:142	bleeding	135:142	bleeding	135:142	Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor.					
36314398	1	17	from	conflicts	158:166	arg1	factor					232:237	a major life-threatening factor	207:237	a major life-threatening factor	207:237	Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor.					
36314398	8	18	theme	minimal	1362:1368	arg1	loss					1381:1384	minimal ingredient loss	1362:1384	minimal ingredient loss	1362:1384	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	0	19	theme	Hemostatic	99:108	arg1	Dressing					110:117	Robust and High-Performance Hemostatic Dressing	71:117	Robust and High-Performance Hemostatic Dressing	71:117	Assembly of Clay Nanotubes on Cotton Fibers Mediated by Biopolymer for Robust and High-Performance Hemostatic Dressing.					
36314398	8	20	from	self-assembly	1239:1251	arg1	fibers					1282:1287	textile fibers	1274:1287	textile fibers	1274:1287	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	7	21	dep	nanoclay	1133:1140	arg1	the					1129:1131	the	1129:1131	the	1129:1131	The hemostatic mechanism of the dressing is attributed to activating platelets, locally concentrating clotting components in the nanoclay, halloysite coagulation factors, and alginate cross-linked with Ca2+ .					
36314398	4	22	theme	Ca2+	634:637	arg1	crosslinking					639:650	Ca2+ crosslinking	634:650	Ca2+ crosslinking	634:650	Here, an efficient clay hemostat of halloysite nanotubes is tightly bound onto commercial cotton fibers, which is capillary mediated by biopolymer alginate with Ca2+ crosslinking.					
36314398	3	23	theme	hemostats	374:382	arg1	peel-off					362:369	the peel-off	358:369	the peel-off of hemostats such as kaolinite sheets from the cotton fibers	358:430	However, the peel-off of hemostats such as kaolinite sheets from the cotton fibers often poses a risk of distal thrombosis.					
36314398	6	24	theme	Combat	869:874	arg1	gauze					876:880	commercial hemostat QuikClot Combat gauze	840:880	commercial hemostat QuikClot Combat gauze	840:880	Compared with commercial hemostat QuikClot Combat gauze, halloysite-alginate-cotton composite dressing exhibits hemostatic properties both in vivo and in vitro with high safety.					
36314398	9	25	with	civil	1521:1525	arg1	risks					1560:1564	low health risks	1549:1564	low health risks	1549:1564	The robust dressing based on halloysite tightly bounded cotton shows great potential for military, medical, and civil bleeding control with low health risks.					
36314398	2	26	theme	bleeding	310:317	arg1	patients					319:326	bleeding patients	310:326	bleeding patients	310:326	Rapid, safe, and convenient hemostasis is critical to the survival of bleeding patients in prehospital care.					
36314398	9	27	with	military	1498:1505	arg1	risks					1560:1564	low health risks	1549:1564	low health risks	1549:1564	The robust dressing based on halloysite tightly bounded cotton shows great potential for military, medical, and civil bleeding control with low health risks.					
36314398	1	28	theme	surgical	184:191	arg1	procedures					193:202	surgical procedures	184:202	surgical procedures	184:202	Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor.					
36314398	7	29	theme	activating	1062:1071	arg1	platelets					1073:1081	activating platelets	1062:1081	activating platelets	1062:1081	The hemostatic mechanism of the dressing is attributed to activating platelets, locally concentrating clotting components in the nanoclay, halloysite coagulation factors, and alginate cross-linked with Ca2+ .					
36314398	9	30	dep	military	1498:1505	arg1	control					1536:1542	bleeding control	1527:1542	bleeding control	1527:1542	The robust dressing based on halloysite tightly bounded cotton shows great potential for military, medical, and civil bleeding control with low health risks.					
36314398	7	31	theme	clotting	1106:1113	arg1	components					1115:1124	clotting components	1106:1124	clotting components in the nanoclay, halloysite coagulation factors, and alginate cross-linked with Ca2+	1106:1209	The hemostatic mechanism of the dressing is attributed to activating platelets, locally concentrating clotting components in the nanoclay, halloysite coagulation factors, and alginate cross-linked with Ca2+ .					
36314398	3	32	theme	cotton	418:423	arg1	fibers					425:430	the cotton fibers	414:430	the cotton fibers	414:430	However, the peel-off of hemostats such as kaolinite sheets from the cotton fibers often poses a risk of distal thrombosis.					
36314398	0	33	theme	Nanotubes	17:25	arg1	Assembly					0:7	Assembly	0:7	Assembly of Clay Nanotubes on Cotton Fibers	0:42	Assembly of Clay Nanotubes on Cotton Fibers Mediated by Biopolymer for Robust and High-Performance Hemostatic Dressing.					
36314398	8	34	theme	balanced	1327:1334	arg1	activity					1352:1359	balanced high hemostatic activity	1327:1359	balanced high hemostatic activity	1327:1359	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	9	35	theme	great	1478:1482	arg1	potential					1484:1492	great potential	1478:1492	great potential for military, medical, and civil bleeding control with low health risks	1478:1564	The robust dressing based on halloysite tightly bounded cotton shows great potential for military, medical, and civil bleeding control with low health risks.					
36314398	9	36	theme	robust	1413:1418	arg1	dressing					1420:1427	The robust dressing	1409:1427	The robust dressing based on halloysite tightly bounded cotton	1409:1470	The robust dressing based on halloysite tightly bounded cotton shows great potential for military, medical, and civil bleeding control with low health risks.					
36314398	4	37	theme	biopolymer	609:618	arg1	alginate					620:627	biopolymer alginate	609:627	biopolymer alginate with Ca2+ crosslinking	609:650	Here, an efficient clay hemostat of halloysite nanotubes is tightly bound onto commercial cotton fibers, which is capillary mediated by biopolymer alginate with Ca2+ crosslinking.					
36314398	7	38	from	components	1115:1124	arg1	nanoclay					1133:1140	nanoclay	1133:1140	nanoclay	1133:1140	The hemostatic mechanism of the dressing is attributed to activating platelets, locally concentrating clotting components in the nanoclay, halloysite coagulation factors, and alginate cross-linked with Ca2+ .					
36314398	7	38	from	components	1115:1124	arg1	factors					1166:1172	halloysite coagulation factors	1143:1172	halloysite coagulation factors	1143:1172	The hemostatic mechanism of the dressing is attributed to activating platelets, locally concentrating clotting components in the nanoclay, halloysite coagulation factors, and alginate cross-linked with Ca2+ .					
36314398	7	38	from	components	1115:1124	arg1	alginate					1179:1186	alginate	1179:1186	alginate	1179:1186	The hemostatic mechanism of the dressing is attributed to activating platelets, locally concentrating clotting components in the nanoclay, halloysite coagulation factors, and alginate cross-linked with Ca2+ .					
36314398	9	39	theme	bounded	1457:1463	arg1	cotton					1465:1470	halloysite tightly bounded cotton	1438:1470	halloysite tightly bounded cotton	1438:1470	The robust dressing based on halloysite tightly bounded cotton shows great potential for military, medical, and civil bleeding control with low health risks.					
36314398	6	40	theme	QuikClot	860:867	arg1	gauze					876:880	commercial hemostat QuikClot Combat gauze	840:880	commercial hemostat QuikClot Combat gauze	840:880	Compared with commercial hemostat QuikClot Combat gauze, halloysite-alginate-cotton composite dressing exhibits hemostatic properties both in vivo and in vitro with high safety.					
36314398	9	41	theme	low	1549:1551	arg1	risks					1560:1564	low health risks	1549:1564	low health risks	1549:1564	The robust dressing based on halloysite tightly bounded cotton shows great potential for military, medical, and civil bleeding control with low health risks.					
36314398	0	42	theme	Cotton	30:35	arg1	Fibers					37:42	Cotton Fibers	30:42	Cotton Fibers	30:42	Assembly of Clay Nanotubes on Cotton Fibers Mediated by Biopolymer for Robust and High-Performance Hemostatic Dressing.					
36314398	9	43	theme	bleeding	1527:1534	arg1	control					1536:1542	bleeding control	1527:1542	bleeding control	1527:1542	The robust dressing based on halloysite tightly bounded cotton shows great potential for military, medical, and civil bleeding control with low health risks.					
36314398	5	44	theme	high	706:709	arg1	activity					724:731	high procoagulant activity	706:731	high procoagulant activity	706:731	The robust clay nanotube dressing materials maintain high procoagulant activity after harsh water treatment, and only a few residuals of halloysite exist in the wound area.					
36314398	6	45	theme	hemostat	851:858	arg1	gauze					876:880	commercial hemostat QuikClot Combat gauze	840:880	commercial hemostat QuikClot Combat gauze	840:880	Compared with commercial hemostat QuikClot Combat gauze, halloysite-alginate-cotton composite dressing exhibits hemostatic properties both in vivo and in vitro with high safety.					
36314398	8	46	theme	textile	1274:1280	arg1	fibers					1282:1287	textile fibers	1274:1287	textile fibers	1274:1287	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	1	47	theme	major	209:213	arg1	bleeding					135:142	bleeding	135:142	bleeding	135:142	Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor.					
36314398	1	47	theme	major	209:213	arg1	factor					232:237	a major life-threatening factor	207:237	a major life-threatening factor	207:237	Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor.					
36314398	8	48	theme	robust	1232:1237	arg1	self-assembly					1239:1251	robust self-assembly	1232:1251	robust self-assembly of clay nanotubes on textile fibers	1232:1287	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	8	49	theme	hemostatic	1302:1311	arg1	material					1313:1320	a hemostatic material	1300:1320	a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility	1300:1406	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	5	50	theme	procoagulant	711:722	arg1	activity					724:731	high procoagulant activity	706:731	high procoagulant activity	706:731	The robust clay nanotube dressing materials maintain high procoagulant activity after harsh water treatment, and only a few residuals of halloysite exist in the wound area.					
36314398	6	51	theme	commercial	840:849	arg1	gauze					876:880	commercial hemostat QuikClot Combat gauze	840:880	commercial hemostat QuikClot Combat gauze	840:880	Compared with commercial hemostat QuikClot Combat gauze, halloysite-alginate-cotton composite dressing exhibits hemostatic properties both in vivo and in vitro with high safety.					
36314398	2	52	theme	Rapid	240:244	arg1	hemostasis					268:277	Rapid, safe, and convenient hemostasis	240:277	Rapid, safe, and convenient hemostasis	240:277	Rapid, safe, and convenient hemostasis is critical to the survival of bleeding patients in prehospital care.					
36314398	8	53	from	fibers	1282:1287	arg1	self-assembly					1239:1251	robust self-assembly	1232:1251	robust self-assembly of clay nanotubes on textile fibers	1232:1287	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	1	54	theme	life-threatening	215:230	arg1	bleeding					135:142	bleeding	135:142	bleeding	135:142	Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor.					
36314398	1	54	theme	life-threatening	215:230	arg1	factor					232:237	a major life-threatening factor	207:237	a major life-threatening factor	207:237	Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor.					
36314398	7	55	theme	coagulation	1154:1164	arg1	factors					1166:1172	halloysite coagulation factors	1143:1172	halloysite coagulation factors	1143:1172	The hemostatic mechanism of the dressing is attributed to activating platelets, locally concentrating clotting components in the nanoclay, halloysite coagulation factors, and alginate cross-linked with Ca2+ .					
36314398	5	56	theme	robust	657:662	arg1	materials					687:695	The robust clay nanotube dressing materials	653:695	The robust clay nanotube dressing materials	653:695	The robust clay nanotube dressing materials maintain high procoagulant activity after harsh water treatment, and only a few residuals of halloysite exist in the wound area.					
36314398	8	57	with	material	1313:1320	arg1	activity					1352:1359	balanced high hemostatic activity	1327:1359	balanced high hemostatic activity	1327:1359	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	8	57	with	material	1313:1320	arg1	loss					1381:1384	minimal ingredient loss	1362:1384	minimal ingredient loss	1362:1384	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	8	57	with	material	1313:1320	arg1	biocompatibility					1391:1406	biocompatibility	1391:1406	biocompatibility	1391:1406	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	7	58	theme	dressing	1036:1043	arg1	mechanism					1019:1027	The hemostatic mechanism	1004:1027	The hemostatic mechanism of the dressing	1004:1043	The hemostatic mechanism of the dressing is attributed to activating platelets, locally concentrating clotting components in the nanoclay, halloysite coagulation factors, and alginate cross-linked with Ca2+ .					
36314398	3	59	theme	kaolinite	392:400	arg1	sheets					402:407	kaolinite sheets	392:407	kaolinite sheets from the cotton fibers	392:430	However, the peel-off of hemostats such as kaolinite sheets from the cotton fibers often poses a risk of distal thrombosis.					
36314398	4	60	theme	nanotubes	520:528	arg1	hemostat					497:504	an efficient clay hemostat	479:504	an efficient clay hemostat of halloysite nanotubes	479:528	Here, an efficient clay hemostat of halloysite nanotubes is tightly bound onto commercial cotton fibers, which is capillary mediated by biopolymer alginate with Ca2+ crosslinking.					
36314398	5	61	theme	clay	664:667	arg1	materials					687:695	The robust clay nanotube dressing materials	653:695	The robust clay nanotube dressing materials	653:695	The robust clay nanotube dressing materials maintain high procoagulant activity after harsh water treatment, and only a few residuals of halloysite exist in the wound area.					
36314398	7	62	theme	hemostatic	1008:1017	arg1	mechanism					1019:1027	The hemostatic mechanism	1004:1027	The hemostatic mechanism of the dressing	1004:1043	The hemostatic mechanism of the dressing is attributed to activating platelets, locally concentrating clotting components in the nanoclay, halloysite coagulation factors, and alginate cross-linked with Ca2+ .					
36314398	2	63	theme	prehospital	331:341	arg1	care					343:346	prehospital care	331:346	prehospital care	331:346	Rapid, safe, and convenient hemostasis is critical to the survival of bleeding patients in prehospital care.					
36314398	4	64	theme	halloysite	509:518	arg1	nanotubes					520:528	halloysite nanotubes	509:528	halloysite nanotubes	509:528	Here, an efficient clay hemostat of halloysite nanotubes is tightly bound onto commercial cotton fibers, which is capillary mediated by biopolymer alginate with Ca2+ crosslinking.					
36314398	5	65	theme	nanotube	669:676	arg1	materials					687:695	The robust clay nanotube dressing materials	653:695	The robust clay nanotube dressing materials	653:695	The robust clay nanotube dressing materials maintain high procoagulant activity after harsh water treatment, and only a few residuals of halloysite exist in the wound area.					
36314398	4	66	with	alginate	620:627	arg1	crosslinking					639:650	Ca2+ crosslinking	634:650	Ca2+ crosslinking	634:650	Here, an efficient clay hemostat of halloysite nanotubes is tightly bound onto commercial cotton fibers, which is capillary mediated by biopolymer alginate with Ca2+ crosslinking.					
36314398	2	67	theme	convenient	257:266	arg1	hemostasis					268:277	Rapid, safe, and convenient hemostasis	240:277	Rapid, safe, and convenient hemostasis	240:277	Rapid, safe, and convenient hemostasis is critical to the survival of bleeding patients in prehospital care.					
36314398	8	68	from	nanotubes	1261:1269	arg1	fibers					1282:1287	textile fibers	1274:1287	textile fibers	1274:1287	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	3	69	theme	distal	454:459	arg1	thrombosis					461:470	distal thrombosis	454:470	distal thrombosis	454:470	However, the peel-off of hemostats such as kaolinite sheets from the cotton fibers often poses a risk of distal thrombosis.					
36314398	5	70	theme	dressing	678:685	arg1	materials					687:695	The robust clay nanotube dressing materials	653:695	The robust clay nanotube dressing materials	653:695	The robust clay nanotube dressing materials maintain high procoagulant activity after harsh water treatment, and only a few residuals of halloysite exist in the wound area.					
36314398	4	71	theme	efficient	482:490	arg1	hemostat					497:504	an efficient clay hemostat	479:504	an efficient clay hemostat of halloysite nanotubes	479:528	Here, an efficient clay hemostat of halloysite nanotubes is tightly bound onto commercial cotton fibers, which is capillary mediated by biopolymer alginate with Ca2+ crosslinking.					
36314398	3	72	theme	thrombosis	461:470	arg1	risk					446:449	a risk	444:449	a risk of distal thrombosis	444:470	However, the peel-off of hemostats such as kaolinite sheets from the cotton fibers often poses a risk of distal thrombosis.					
36314398	8	73	theme	high	1336:1339	arg1	activity					1352:1359	balanced high hemostatic activity	1327:1359	balanced high hemostatic activity	1327:1359	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	1	74	from	procedures	193:202	arg1	bleeding					135:142	bleeding	135:142	bleeding	135:142	Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor.					
36314398	1	74	from	procedures	193:202	arg1	factor					232:237	a major life-threatening factor	207:237	a major life-threatening factor	207:237	Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor.					
36314398	1	75	theme	military	149:156	arg1	conflicts					158:166	military conflicts	149:166	military conflicts	149:166	Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor.					
36314398	8	76	theme	nanotubes	1261:1269	arg1	self-assembly					1239:1251	robust self-assembly	1232:1251	robust self-assembly of clay nanotubes on textile fibers	1232:1287	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	6	77	theme	composite	910:918	arg1	dressing					920:927	halloysite-alginate-cotton composite dressing	883:927	halloysite-alginate-cotton composite dressing	883:927	Compared with commercial hemostat QuikClot Combat gauze, halloysite-alginate-cotton composite dressing exhibits hemostatic properties both in vivo and in vitro with high safety.					
36314398	8	78	theme	clay	1256:1259	arg1	nanotubes					1261:1269	clay nanotubes	1256:1269	clay nanotubes on textile fibers	1256:1287	This work inspires robust self-assembly of clay nanotubes on textile fibers and offers a hemostatic material with balanced high hemostatic activity, minimal ingredient loss, and biocompatibility.					
36314398	6	79	theme	hemostatic	938:947	arg1	properties					949:958	hemostatic properties	938:958	hemostatic properties	938:958	Compared with commercial hemostat QuikClot Combat gauze, halloysite-alginate-cotton composite dressing exhibits hemostatic properties both in vivo and in vitro with high safety.					
36314398	2	80	theme	safe	247:250	arg1	hemostasis					268:277	Rapid, safe, and convenient hemostasis	240:277	Rapid, safe, and convenient hemostasis	240:277	Rapid, safe, and convenient hemostasis is critical to the survival of bleeding patients in prehospital care.					
36314398	3	81	from	fibers	425:430	arg1	sheets					402:407	kaolinite sheets	392:407	kaolinite sheets from the cotton fibers	392:430	However, the peel-off of hemostats such as kaolinite sheets from the cotton fibers often poses a risk of distal thrombosis.					
36314398	5	82	theme	halloysite	790:799	arg1	residuals					777:785	only a few residuals	766:785	only a few residuals of halloysite	766:799	The robust clay nanotube dressing materials maintain high procoagulant activity after harsh water treatment, and only a few residuals of halloysite exist in the wound area.					
36314398	2	83	from	survival	298:305	arg1	care					343:346	prehospital care	331:346	prehospital care	331:346	Rapid, safe, and convenient hemostasis is critical to the survival of bleeding patients in prehospital care.					
36314398	9	84	theme	halloysite	1438:1447	arg1	cotton					1465:1470	halloysite tightly bounded cotton	1438:1470	halloysite tightly bounded cotton	1438:1470	The robust dressing based on halloysite tightly bounded cotton shows great potential for military, medical, and civil bleeding control with low health risks.					
36604628	3	0	theme	metabolic	559:567	arg1	benefits					569:576	substantial host metabolic benefits	542:576	substantial host metabolic benefits	542:576	Here, we observed that prebiotics associated with L. mesenteroides-produced exopolysaccharides (EPS) demonstrate substantial host metabolic benefits.					
36604628	5	1	theme	important	849:857	arg1	mechanism					859:867	an important mechanism	846:867	an important mechanism that accounts for the effects of diet, prebiotics, and probiotics on energy homeostasis and suggests an approach for preventing lifestyle-related diseases by targeting bacterial EPS	846:1049	Our findings reveal an important mechanism that accounts for the effects of diet, prebiotics, and probiotics on energy homeostasis and suggests an approach for preventing lifestyle-related diseases by targeting bacterial EPS.					
36604628	4	2	theme	L.	579:580	arg1	α-glucan					628:635	an indigestible α-glucan	612:635	an indigestible α-glucan	612:635	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	4	2	theme	L.	579:580	arg1	EPS					605:607	L. mesenteroides-produced EPS	579:607	L. mesenteroides-produced EPS	579:607	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	2	3	theme	action	390:395	arg1	mechanism					377:385	the exact mechanism	367:385	the exact mechanism of action of these foods	367:410	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	2	4	theme	Leuconostoc	331:341	arg1	mesenteroides					343:355	Leuconostoc mesenteroides	331:355	Leuconostoc mesenteroides	331:355	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	4	5	theme	purified	656:663	arg1	form					665:668	the purified form	652:668	the purified form of EPS	652:675	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	4	6	theme	fatty	774:778	arg1	acids					780:784	EPS-derived gut microbial short-chain fatty acids	736:784	EPS-derived gut microbial short-chain fatty acids	736:784	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	5	7	theme	prebiotics	908:917	arg1	effects					891:897	the effects	887:897	the effects of diet, prebiotics, and probiotics on energy homeostasis	887:955	Our findings reveal an important mechanism that accounts for the effects of diet, prebiotics, and probiotics on energy homeostasis and suggests an approach for preventing lifestyle-related diseases by targeting bacterial EPS.					
36604628	4	8	theme	glucose	686:692	arg1	metabolism					694:703	glucose metabolism	686:703	glucose metabolism	686:703	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	5	9	theme	energy	938:943	arg1	homeostasis					945:955	energy homeostasis	938:955	energy homeostasis	938:955	Our findings reveal an important mechanism that accounts for the effects of diet, prebiotics, and probiotics on energy homeostasis and suggests an approach for preventing lifestyle-related diseases by targeting bacterial EPS.					
36604628	5	10	theme	probiotics	924:933	arg1	effects					891:897	the effects	887:897	the effects of diet, prebiotics, and probiotics on energy homeostasis	887:955	Our findings reveal an important mechanism that accounts for the effects of diet, prebiotics, and probiotics on energy homeostasis and suggests an approach for preventing lifestyle-related diseases by targeting bacterial EPS.					
36604628	2	11	theme	bacteria	311:318	arg1	action					281:286	the fermentative action	264:286	the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides	264:355	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	1	12	theme	bacterial	208:216	arg1	fermentation					218:229	bacterial fermentation	208:229	bacterial fermentation	208:229	Fermented foods demonstrate remarkable health benefits owing to probiotic bacteria or microproducts produced via bacterial fermentation.					
36604628	0	13	theme	metabolic	5:13	arg1	benefits					15:22	Host metabolic benefits	0:22	Host metabolic benefits of prebiotic exopolysaccharides	0:54	Host metabolic benefits of prebiotic exopolysaccharides produced by Leuconostoc mesenteroides.					
36604628	5	14	from	effects	891:897	arg1	homeostasis					945:955	energy homeostasis	938:955	energy homeostasis	938:955	Our findings reveal an important mechanism that accounts for the effects of diet, prebiotics, and probiotics on energy homeostasis and suggests an approach for preventing lifestyle-related diseases by targeting bacterial EPS.					
36604628	4	15	theme	gut	748:750	arg1	acids					780:784	EPS-derived gut microbial short-chain fatty acids	736:784	EPS-derived gut microbial short-chain fatty acids	736:784	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	0	16	theme	Host	0:3	arg1	benefits					15:22	Host metabolic benefits	0:22	Host metabolic benefits of prebiotic exopolysaccharides	0:54	Host metabolic benefits of prebiotic exopolysaccharides produced by Leuconostoc mesenteroides.					
36604628	4	17	theme	microbial	803:811	arg1	composition					813:823	gut microbial composition	799:823	gut microbial composition	799:823	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	4	18	theme	EPS-derived	736:746	arg1	acids					780:784	EPS-derived gut microbial short-chain fatty acids	736:784	EPS-derived gut microbial short-chain fatty acids	736:784	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	4	19	theme	indigestible	615:626	arg1	α-glucan					628:635	an indigestible α-glucan	612:635	an indigestible α-glucan	612:635	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	4	19	theme	indigestible	615:626	arg1	EPS					605:607	L. mesenteroides-produced EPS	579:607	L. mesenteroides-produced EPS	579:607	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	1	20	theme	remarkable	123:132	arg1	benefits					141:148	remarkable health benefits	123:148	remarkable health benefits owing to probiotic bacteria or microproducts produced via bacterial fermentation	123:229	Fermented foods demonstrate remarkable health benefits owing to probiotic bacteria or microproducts produced via bacterial fermentation.					
36604628	5	21	theme	diet	902:905	arg1	effects					891:897	the effects	887:897	the effects of diet, prebiotics, and probiotics on energy homeostasis	887:955	Our findings reveal an important mechanism that accounts for the effects of diet, prebiotics, and probiotics on energy homeostasis and suggests an approach for preventing lifestyle-related diseases by targeting bacterial EPS.					
36604628	5	22	theme	lifestyle-related	997:1013	arg1	diseases					1015:1022	lifestyle-related diseases	997:1022	lifestyle-related diseases	997:1022	Our findings reveal an important mechanism that accounts for the effects of diet, prebiotics, and probiotics on energy homeostasis and suggests an approach for preventing lifestyle-related diseases by targeting bacterial EPS.					
36604628	1	23	theme	health	134:139	arg1	benefits					141:148	remarkable health benefits	123:148	remarkable health benefits owing to probiotic bacteria or microproducts produced via bacterial fermentation	123:229	Fermented foods demonstrate remarkable health benefits owing to probiotic bacteria or microproducts produced via bacterial fermentation.					
36604628	4	24	theme	short-chain	762:772	arg1	acids					780:784	EPS-derived gut microbial short-chain fatty acids	736:784	EPS-derived gut microbial short-chain fatty acids	736:784	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	0	25	theme	exopolysaccharides	37:54	arg1	benefits					15:22	Host metabolic benefits	0:22	Host metabolic benefits of prebiotic exopolysaccharides	0:54	Host metabolic benefits of prebiotic exopolysaccharides produced by Leuconostoc mesenteroides.					
36604628	4	26	theme	energy	709:714	arg1	homeostasis					716:726	energy homeostasis	709:726	energy homeostasis	709:726	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	3	27	theme	mesenteroides-produced	482:503	arg1	exopolysaccharides					505:522	L. mesenteroides-produced exopolysaccharides	479:522	L. mesenteroides-produced exopolysaccharides (EPS)	479:528	Here, we observed that prebiotics associated with L. mesenteroides-produced exopolysaccharides (EPS) demonstrate substantial host metabolic benefits.					
36604628	3	27	theme	mesenteroides-produced	482:503	arg1	EPS					525:527	EPS	525:527	EPS	525:527	Here, we observed that prebiotics associated with L. mesenteroides-produced exopolysaccharides (EPS) demonstrate substantial host metabolic benefits.					
36604628	0	28	theme	prebiotic	27:35	arg1	exopolysaccharides					37:54	prebiotic exopolysaccharides	27:54	prebiotic exopolysaccharides	27:54	Host metabolic benefits of prebiotic exopolysaccharides produced by Leuconostoc mesenteroides.					
36604628	2	29	theme	fermentative	268:279	arg1	action					281:286	the fermentative action	264:286	the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides	264:355	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	4	30	theme	mesenteroides-produced	582:603	arg1	α-glucan					628:635	an indigestible α-glucan	612:635	an indigestible α-glucan	612:635	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	4	30	theme	mesenteroides-produced	582:603	arg1	EPS					605:607	L. mesenteroides-produced EPS	579:607	L. mesenteroides-produced EPS	579:607	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	3	31	theme	substantial	542:552	arg1	benefits					569:576	substantial host metabolic benefits	542:576	substantial host metabolic benefits	542:576	Here, we observed that prebiotics associated with L. mesenteroides-produced exopolysaccharides (EPS) demonstrate substantial host metabolic benefits.					
36604628	4	32	theme	form	665:668	arg1	intake					642:647	intake	642:647	intake of the purified form of EPS	642:675	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	3	33	theme	host	554:557	arg1	benefits					569:576	substantial host metabolic benefits	542:576	substantial host metabolic benefits	542:576	Here, we observed that prebiotics associated with L. mesenteroides-produced exopolysaccharides (EPS) demonstrate substantial host metabolic benefits.					
36604628	2	34	theme	acid	306:309	arg1	bacteria					311:318	several lactic acid bacteria	291:318	several lactic acid bacteria	291:318	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	2	34	theme	acid	306:309	arg1	mesenteroides					343:355	Leuconostoc mesenteroides	331:355	Leuconostoc mesenteroides	331:355	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	4	35	link	EPS-derived	736:746	arg1	acids					780:784	EPS-derived gut microbial short-chain fatty acids	736:784	EPS-derived gut microbial short-chain fatty acids	736:784	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	2	36	theme	exact	371:375	arg1	mechanism					377:385	the exact mechanism	367:385	the exact mechanism of action of these foods	367:410	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	5	37	theme	bacterial	1037:1045	arg1	EPS					1047:1049	bacterial EPS	1037:1049	bacterial EPS	1037:1049	Our findings reveal an important mechanism that accounts for the effects of diet, prebiotics, and probiotics on energy homeostasis and suggests an approach for preventing lifestyle-related diseases by targeting bacterial EPS.					
36604628	1	38	theme	Fermented	95:103	arg1	foods					105:109	Fermented foods	95:109	Fermented foods	95:109	Fermented foods demonstrate remarkable health benefits owing to probiotic bacteria or microproducts produced via bacterial fermentation.					
36604628	2	39	theme	lactic	299:304	arg1	bacteria					311:318	several lactic acid bacteria	291:318	several lactic acid bacteria	291:318	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	2	39	theme	lactic	299:304	arg1	mesenteroides					343:355	Leuconostoc mesenteroides	331:355	Leuconostoc mesenteroides	331:355	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	1	40	theme	probiotic	159:167	arg1	bacteria					169:176	bacteria	169:176	bacteria	169:176	Fermented foods demonstrate remarkable health benefits owing to probiotic bacteria or microproducts produced via bacterial fermentation.					
36604628	4	41	theme	gut	799:801	arg1	composition					813:823	gut microbial composition	799:823	gut microbial composition	799:823	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	2	42	theme	Fermented	232:240	arg1	foods					242:246	Fermented foods	232:246	Fermented foods	232:246	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	2	43	theme	several	291:297	arg1	bacteria					311:318	several lactic acid bacteria	291:318	several lactic acid bacteria	291:318	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	2	43	theme	several	291:297	arg1	mesenteroides					343:355	Leuconostoc mesenteroides	331:355	Leuconostoc mesenteroides	331:355	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	2	44	theme	foods	406:410	arg1	action					390:395	action	390:395	action of these foods	390:410	Fermented foods are produced by the fermentative action of several lactic acid bacteria, including Leuconostoc mesenteroides; however, the exact mechanism of action of these foods remains unclear.					
36604628	3	45	theme	L.	479:480	arg1	exopolysaccharides					505:522	L. mesenteroides-produced exopolysaccharides	479:522	L. mesenteroides-produced exopolysaccharides (EPS)	479:528	Here, we observed that prebiotics associated with L. mesenteroides-produced exopolysaccharides (EPS) demonstrate substantial host metabolic benefits.					
36604628	3	45	theme	L.	479:480	arg1	EPS					525:527	EPS	525:527	EPS	525:527	Here, we observed that prebiotics associated with L. mesenteroides-produced exopolysaccharides (EPS) demonstrate substantial host metabolic benefits.					
36604628	0	46	theme	Leuconostoc	68:78	arg1	mesenteroides					80:92	Leuconostoc mesenteroides	68:92	Leuconostoc mesenteroides	68:92	Host metabolic benefits of prebiotic exopolysaccharides produced by Leuconostoc mesenteroides.					
36604628	4	47	theme	microbial	752:760	arg1	acids					780:784	EPS-derived gut microbial short-chain fatty acids	736:784	EPS-derived gut microbial short-chain fatty acids	736:784	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
36604628	4	48	theme	EPS	673:675	arg1	form					665:668	the purified form	652:668	the purified form of EPS	652:675	L. mesenteroides-produced EPS is an indigestible α-glucan, and intake of the purified form of EPS improved glucose metabolism and energy homeostasis through EPS-derived gut microbial short-chain fatty acids, and changed gut microbial composition.					
37003502	0	0	theme	acid	89:92	arg1	nanoparticle-hydrogel					103:123	an analogous hyaluronic acid chitosan nanoparticle-hydrogel	65:123	an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice	65:157	Immunological evaluation of a recombinant vaccine delivered with an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice.					
37003502	11	1	theme	novel	1697:1701	arg1	AHACNP-HG					1712:1720	the novel adjuvant AHACNP-HG	1693:1720	the novel adjuvant AHACNP-HG	1693:1720	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	10	2	theme	survival	1504:1511	arg1	time					1513:1516	the survival time	1500:1516	the survival time of mice	1500:1524	Additionally, after challenge with T. gondii tachyzoite, AHACNP-HG/rMIC6/rROP18 inoculation prolonged the survival time of mice.					
37003502	11	3	theme	T.	1668:1669	arg1	infection					1678:1686	T. gondii infection	1668:1686	T. gondii infection	1668:1686	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	2	4	theme	recombinant	334:344	arg1	vaccine					346:352	a recombinant vaccine	332:352	a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18)	332:446	This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18).					
37003502	11	5	used	used	1818:1821	arg2	system					1843:1848	a novel delivery system	1826:1848	a novel delivery system in vaccine development	1826:1871	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	11	5	used	used	1818:1821	arg2	it					1808:1809	it	1808:1809	it	1808:1809	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	6	6	theme	anti-infection	973:986	arg1	effects					988:994	The immunological and anti-infection effects	951:994	The immunological and anti-infection effects of AHACNP-HG/rMIC6/rROP18	951:1020	The immunological and anti-infection effects of AHACNP-HG/rMIC6/rROP18 were examined in the mice model.					
37003502	0	7	theme	hyaluronic	78:87	arg1	nanoparticle-hydrogel					103:123	an analogous hyaluronic acid chitosan nanoparticle-hydrogel	65:123	an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice	65:157	Immunological evaluation of a recombinant vaccine delivered with an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice.					
37003502	11	8	theme	novel	1828:1832	arg1	system					1843:1848	a novel delivery system	1826:1848	a novel delivery system in vaccine development	1826:1871	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	11	8	theme	novel	1828:1832	arg1	it					1808:1809	it	1808:1809	it	1808:1809	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	11	9	theme	mouse	1774:1778	arg1	model					1780:1784	mouse model	1774:1784	mouse model	1774:1784	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	8	10	theme	immune	1260:1265	arg1	response					1267:1274	a mixed Th1/Th2 cellular immune response	1235:1274	a mixed Th1/Th2 cellular immune response accompanied by an increased level of the cytokines, IFN-γ and IL-10	1235:1342	AHACNP-HG/rMIC6/rROP18 vaccine enhanced a mixed Th1/Th2 cellular immune response accompanied by an increased level of the cytokines, IFN-γ and IL-10.					
37003502	7	11	contain	possessed	1124:1132	arg2	biosecurity					1134:1144	biosecurity	1134:1144	biosecurity with excellent protein control-release property	1134:1192	RESULTS AHACNP-HG presented a characteristic of composite system and possessed biosecurity with excellent protein control-release property.					
37003502	7	11	contain	possessed	1124:1132	arg1	RESULTS					1055:1061	RESULTS	1055:1061	RESULTS	1055:1061	RESULTS AHACNP-HG presented a characteristic of composite system and possessed biosecurity with excellent protein control-release property.					
37003502	3	12	theme	immune	578:583	arg1	protection					585:594	its immune protection	574:594	its immune protection	574:594	The vaccine was delivered with a novel vector, named analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG) and its immune protection was evaluated.					
37003502	7	13	theme	composite	1103:1111	arg1	system					1113:1118	composite system	1103:1118	composite system	1103:1118	RESULTS AHACNP-HG presented a characteristic of composite system and possessed biosecurity with excellent protein control-release property.					
37003502	11	14	theme	immune	1600:1605	arg1	response					1607:1614	strong immune response	1593:1614	strong immune response	1593:1614	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	4	15	theme	magnetic	729:736	arg1	stirring					738:745	magnetic stirring	729:745	magnetic stirring	729:745	METHODS The recombinant MIC6 and ROP18 proteins were obtained by affinity chromatography and loaded onto AHACNP-HG by magnetic stirring.					
37003502	11	16	theme	mixed	1562:1566	arg1	rMIC6					1568:1572	rMIC6	1568:1572	rMIC6	1568:1572	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	6	17	theme	immunological	955:967	arg1	effects					988:994	The immunological and anti-infection effects	951:994	The immunological and anti-infection effects of AHACNP-HG/rMIC6/rROP18	951:1020	The immunological and anti-infection effects of AHACNP-HG/rMIC6/rROP18 were examined in the mice model.					
37003502	0	18	theme	chitosan	94:101	arg1	nanoparticle-hydrogel					103:123	an analogous hyaluronic acid chitosan nanoparticle-hydrogel	65:123	an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice	65:157	Immunological evaluation of a recombinant vaccine delivered with an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice.					
37003502	7	19	theme	system	1113:1118	arg1	characteristic					1085:1098	a characteristic	1083:1098	a characteristic of composite system	1083:1118	RESULTS AHACNP-HG presented a characteristic of composite system and possessed biosecurity with excellent protein control-release property.					
37003502	0	20	theme	Toxoplasma	133:142	arg1	gondii					144:149	Toxoplasma gondii	133:149	Toxoplasma gondii	133:149	Immunological evaluation of a recombinant vaccine delivered with an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice.					
37003502	5	21	theme	nanoparticle	849:860	arg1	size					862:865	nanoparticle size	849:865	nanoparticle size	849:865	The characterizations of AHACNP-HG were investigated, including its structure, rheological property, nanoparticle size and zeta potential, its ability to release protein in vitro and toxicology in vivo.					
37003502	2	22	theme	microneme	376:384	arg1	protein					386:392	microneme protein 6	376:394	microneme protein 6 (TgMIC6)	376:403	This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18).					
37003502	2	22	theme	microneme	376:384	arg1	TgMIC6					397:402	TgMIC6	397:402	TgMIC6	397:402	This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18).					
37003502	5	23	theme	AHACNP-HG	773:781	arg1	characterizations					752:768	The characterizations	748:768	The characterizations of AHACNP-HG	748:781	The characterizations of AHACNP-HG were investigated, including its structure, rheological property, nanoparticle size and zeta potential, its ability to release protein in vitro and toxicology in vivo.					
37003502	11	24	theme	adjuvant	1703:1710	arg1	AHACNP-HG					1712:1720	the novel adjuvant AHACNP-HG	1693:1720	the novel adjuvant AHACNP-HG	1693:1720	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	7	25	theme	excellent	1151:1159	arg1	property					1185:1192	excellent protein control-release property	1151:1192	excellent protein control-release property	1151:1192	RESULTS AHACNP-HG presented a characteristic of composite system and possessed biosecurity with excellent protein control-release property.					
37003502	10	26	theme	AHACNP-HG/rMIC6/rROP18	1455:1476	arg1	inoculation					1478:1488	AHACNP-HG/rMIC6/rROP18 inoculation	1455:1488	AHACNP-HG/rMIC6/rROP18 inoculation	1455:1488	Additionally, after challenge with T. gondii tachyzoite, AHACNP-HG/rMIC6/rROP18 inoculation prolonged the survival time of mice.					
37003502	0	27	theme	Immunological	0:12	arg1	evaluation					14:23	Immunological evaluation	0:23	Immunological evaluation of a recombinant vaccine	0:48	Immunological evaluation of a recombinant vaccine delivered with an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice.					
37003502	8	28	theme	AHACNP-HG/rMIC6/rROP18	1195:1216	arg1	vaccine					1218:1224	AHACNP-HG/rMIC6/rROP18 vaccine	1195:1224	AHACNP-HG/rMIC6/rROP18 vaccine	1195:1224	AHACNP-HG/rMIC6/rROP18 vaccine enhanced a mixed Th1/Th2 cellular immune response accompanied by an increased level of the cytokines, IFN-γ and IL-10.					
37003502	2	29	theme	gondii	412:417	arg1	protein					427:433	T. gondii rhoptry protein 18	409:436	T. gondii rhoptry protein 18 (TgROP18)	409:446	This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18).					
37003502	2	29	theme	gondii	412:417	arg1	TgROP18					439:445	TgROP18	439:445	TgROP18	439:445	This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18).					
37003502	11	30	theme	delivery	1834:1841	arg1	system					1843:1848	a novel delivery system	1826:1848	a novel delivery system in vaccine development	1826:1871	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	11	30	theme	delivery	1834:1841	arg1	it					1808:1809	it	1808:1809	it	1808:1809	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	11	31	from	system	1843:1848	arg1	development					1861:1871	vaccine development	1853:1871	vaccine development	1853:1871	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	10	32	with	challenge	1418:1426	arg1	tachyzoite					1443:1452	T. gondii tachyzoite	1433:1452	T. gondii tachyzoite	1433:1452	Additionally, after challenge with T. gondii tachyzoite, AHACNP-HG/rMIC6/rROP18 inoculation prolonged the survival time of mice.					
37003502	3	33	theme	hyaluronic	511:520	arg1	AHACNP-HG					559:567	AHACNP-HG	559:567	AHACNP-HG	559:567	The vaccine was delivered with a novel vector, named analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG) and its immune protection was evaluated.					
37003502	3	33	theme	hyaluronic	511:520	arg1	nanoparticle-hydrogel					536:556	analogous hyaluronic acid chitosan nanoparticle-hydrogel	501:556	analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG)	501:568	The vaccine was delivered with a novel vector, named analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG) and its immune protection was evaluated.					
37003502	11	34	theme	protective	1637:1646	arg1	role					1648:1651	a certain protective role	1627:1651	a certain protective role	1627:1651	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	2	35	theme	T.	366:367	arg1	protein					386:392	microneme protein 6	376:394	microneme protein 6 (TgMIC6)	376:403	This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18).					
37003502	2	35	theme	T.	366:367	arg1	TgMIC6					397:402	TgMIC6	397:402	TgMIC6	397:402	This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18).					
37003502	2	36	theme	T.	409:410	arg1	protein					427:433	T. gondii rhoptry protein 18	409:436	T. gondii rhoptry protein 18 (TgROP18)	409:446	This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18).					
37003502	2	36	theme	T.	409:410	arg1	TgROP18					439:445	TgROP18	439:445	TgROP18	439:445	This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18).					
37003502	1	37	theme	adverse	261:267	arg1	impacts					269:275	adverse impacts	261:275	adverse impacts on the livestock industry	261:301	BACKGROUND Toxoplasma gondii (T. gondii) is not only a threat to the public health but it also poses adverse impacts on the livestock industry.					
37003502	1	38	from	impacts	269:275	arg1	industry					294:301	the livestock industry	280:301	the livestock industry	280:301	BACKGROUND Toxoplasma gondii (T. gondii) is not only a threat to the public health but it also poses adverse impacts on the livestock industry.					
37003502	3	39	theme	acid	522:525	arg1	AHACNP-HG					559:567	AHACNP-HG	559:567	AHACNP-HG	559:567	The vaccine was delivered with a novel vector, named analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG) and its immune protection was evaluated.					
37003502	3	39	theme	acid	522:525	arg1	nanoparticle-hydrogel					536:556	analogous hyaluronic acid chitosan nanoparticle-hydrogel	501:556	analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG)	501:568	The vaccine was delivered with a novel vector, named analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG) and its immune protection was evaluated.					
37003502	4	40	theme	recombinant	623:633	arg1	MIC6					635:638	The recombinant MIC6	619:638	The recombinant MIC6	619:638	METHODS The recombinant MIC6 and ROP18 proteins were obtained by affinity chromatography and loaded onto AHACNP-HG by magnetic stirring.					
37003502	7	41	theme	control-release	1169:1183	arg1	property					1185:1192	excellent protein control-release property	1151:1192	excellent protein control-release property	1151:1192	RESULTS AHACNP-HG presented a characteristic of composite system and possessed biosecurity with excellent protein control-release property.					
37003502	9	42	theme	stronger	1364:1371	arg1	response					1388:1395	a stronger humoral immune response	1362:1395	a stronger humoral immune response	1362:1395	It also provoked a stronger humoral immune response.					
37003502	8	43	theme	cellular	1251:1258	arg1	response					1267:1274	a mixed Th1/Th2 cellular immune response	1235:1274	a mixed Th1/Th2 cellular immune response accompanied by an increased level of the cytokines, IFN-γ and IL-10	1235:1342	AHACNP-HG/rMIC6/rROP18 vaccine enhanced a mixed Th1/Th2 cellular immune response accompanied by an increased level of the cytokines, IFN-γ and IL-10.					
37003502	10	44	theme	T.	1433:1434	arg1	tachyzoite					1443:1452	T. gondii tachyzoite	1433:1452	T. gondii tachyzoite	1433:1452	Additionally, after challenge with T. gondii tachyzoite, AHACNP-HG/rMIC6/rROP18 inoculation prolonged the survival time of mice.					
37003502	6	45	theme	mice	1043:1046	arg1	model					1048:1052	the mice model	1039:1052	the mice model	1039:1052	The immunological and anti-infection effects of AHACNP-HG/rMIC6/rROP18 were examined in the mice model.					
37003502	11	46	dep	CONCLUSION	1527:1536	arg1	indicated					1547:1555	indicated	1547:1555	indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development	1547:1871	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	9	47	theme	humoral	1373:1379	arg1	response					1388:1395	a stronger humoral immune response	1362:1395	a stronger humoral immune response	1362:1395	It also provoked a stronger humoral immune response.					
37003502	7	48	with	biosecurity	1134:1144	arg1	property					1185:1192	excellent protein control-release property	1151:1192	excellent protein control-release property	1151:1192	RESULTS AHACNP-HG presented a characteristic of composite system and possessed biosecurity with excellent protein control-release property.					
37003502	3	49	theme	analogous	501:509	arg1	AHACNP-HG					559:567	AHACNP-HG	559:567	AHACNP-HG	559:567	The vaccine was delivered with a novel vector, named analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG) and its immune protection was evaluated.					
37003502	3	49	theme	analogous	501:509	arg1	nanoparticle-hydrogel					536:556	analogous hyaluronic acid chitosan nanoparticle-hydrogel	501:556	analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG)	501:568	The vaccine was delivered with a novel vector, named analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG) and its immune protection was evaluated.					
37003502	0	50	theme	vaccine	42:48	arg1	evaluation					14:23	Immunological evaluation	0:23	Immunological evaluation of a recombinant vaccine	0:48	Immunological evaluation of a recombinant vaccine delivered with an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice.					
37003502	9	51	theme	immune	1381:1386	arg1	response					1388:1395	a stronger humoral immune response	1362:1395	a stronger humoral immune response	1362:1395	It also provoked a stronger humoral immune response.					
37003502	10	52	dep	T.	1433:1434	arg1	gondii					1436:1441	gondii	1436:1441	gondii	1436:1441	Additionally, after challenge with T. gondii tachyzoite, AHACNP-HG/rMIC6/rROP18 inoculation prolonged the survival time of mice.					
37003502	11	53	theme	strong	1593:1598	arg1	response					1607:1614	strong immune response	1593:1614	strong immune response	1593:1614	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	8	54	theme	cytokines	1317:1325	arg1	level					1304:1308	an increased level	1291:1308	an increased level of the cytokines, IFN-γ and IL-10	1291:1342	AHACNP-HG/rMIC6/rROP18 vaccine enhanced a mixed Th1/Th2 cellular immune response accompanied by an increased level of the cytokines, IFN-γ and IL-10.					
37003502	0	55	theme	recombinant	30:40	arg1	vaccine					42:48	a recombinant vaccine	28:48	a recombinant vaccine	28:48	Immunological evaluation of a recombinant vaccine delivered with an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice.					
37003502	1	56	theme	livestock	284:292	arg1	industry					294:301	the livestock industry	280:301	the livestock industry	280:301	BACKGROUND Toxoplasma gondii (T. gondii) is not only a threat to the public health but it also poses adverse impacts on the livestock industry.					
37003502	7	57	theme	protein	1161:1167	arg1	property					1185:1192	excellent protein control-release property	1151:1192	excellent protein control-release property	1151:1192	RESULTS AHACNP-HG presented a characteristic of composite system and possessed biosecurity with excellent protein control-release property.					
37003502	8	58	theme	mixed	1237:1241	arg1	response					1267:1274	a mixed Th1/Th2 cellular immune response	1235:1274	a mixed Th1/Th2 cellular immune response accompanied by an increased level of the cytokines, IFN-γ and IL-10	1235:1342	AHACNP-HG/rMIC6/rROP18 vaccine enhanced a mixed Th1/Th2 cellular immune response accompanied by an increased level of the cytokines, IFN-γ and IL-10.					
37003502	5	59	theme	rheological	827:837	arg1	property					839:846	rheological property	827:846	rheological property	827:846	The characterizations of AHACNP-HG were investigated, including its structure, rheological property, nanoparticle size and zeta potential, its ability to release protein in vitro and toxicology in vivo.					
37003502	8	60	theme	Th1/Th2	1243:1249	arg1	response					1267:1274	a mixed Th1/Th2 cellular immune response	1235:1274	a mixed Th1/Th2 cellular immune response accompanied by an increased level of the cytokines, IFN-γ and IL-10	1235:1342	AHACNP-HG/rMIC6/rROP18 vaccine enhanced a mixed Th1/Th2 cellular immune response accompanied by an increased level of the cytokines, IFN-γ and IL-10.					
37003502	11	61	theme	immunogenicity	1745:1758	arg1	properties					1760:1769	some immunogenicity properties	1740:1769	some immunogenicity properties	1740:1769	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	3	62	theme	chitosan	527:534	arg1	AHACNP-HG					559:567	AHACNP-HG	559:567	AHACNP-HG	559:567	The vaccine was delivered with a novel vector, named analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG) and its immune protection was evaluated.					
37003502	3	62	theme	chitosan	527:534	arg1	nanoparticle-hydrogel					536:556	analogous hyaluronic acid chitosan nanoparticle-hydrogel	501:556	analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG)	501:568	The vaccine was delivered with a novel vector, named analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG) and its immune protection was evaluated.					
37003502	2	63	theme	rhoptry	419:425	arg1	protein					427:433	T. gondii rhoptry protein 18	409:436	T. gondii rhoptry protein 18 (TgROP18)	409:446	This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18).					
37003502	2	63	theme	rhoptry	419:425	arg1	TgROP18					439:445	TgROP18	439:445	TgROP18	439:445	This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18).					
37003502	11	64	theme	vaccine	1853:1859	arg1	development					1861:1871	vaccine development	1853:1871	vaccine development	1853:1871	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	10	65	theme	mice	1521:1524	arg1	time					1513:1516	the survival time	1500:1516	the survival time of mice	1500:1524	Additionally, after challenge with T. gondii tachyzoite, AHACNP-HG/rMIC6/rROP18 inoculation prolonged the survival time of mice.					
37003502	11	66	theme	certain	1629:1635	arg1	role					1648:1651	a certain protective role	1627:1651	a certain protective role	1627:1651	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	2	67	dep	T.	366:367	arg1	gondii					369:374	gondii	369:374	gondii	369:374	This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18).					
37003502	8	68	theme	increased	1294:1302	arg1	level					1304:1308	an increased level	1291:1308	an increased level of the cytokines, IFN-γ and IL-10	1291:1342	AHACNP-HG/rMIC6/rROP18 vaccine enhanced a mixed Th1/Th2 cellular immune response accompanied by an increased level of the cytokines, IFN-γ and IL-10.					
37003502	3	69	theme	novel	481:485	arg1	vector					487:492	a novel vector	479:492	a novel vector	479:492	The vaccine was delivered with a novel vector, named analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG) and its immune protection was evaluated.					
37003502	0	70	from	nanoparticle-hydrogel	103:123	arg1	mice					154:157	mice	154:157	mice	154:157	Immunological evaluation of a recombinant vaccine delivered with an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice.					
37003502	1	71	dep	threat	215:220	arg1	T.					190:191	T.	190:191	T.	190:191	BACKGROUND Toxoplasma gondii (T. gondii) is not only a threat to the public health but it also poses adverse impacts on the livestock industry.					
37003502	4	72	theme	affinity	676:683	arg1	chromatography					685:698	affinity chromatography	676:698	affinity chromatography	676:698	METHODS The recombinant MIC6 and ROP18 proteins were obtained by affinity chromatography and loaded onto AHACNP-HG by magnetic stirring.					
37003502	6	73	theme	AHACNP-HG/rMIC6/rROP18	999:1020	arg1	effects					988:994	The immunological and anti-infection effects	951:994	The immunological and anti-infection effects of AHACNP-HG/rMIC6/rROP18	951:1020	The immunological and anti-infection effects of AHACNP-HG/rMIC6/rROP18 were examined in the mice model.					
37003502	0	74	theme	analogous	68:76	arg1	nanoparticle-hydrogel					103:123	an analogous hyaluronic acid chitosan nanoparticle-hydrogel	65:123	an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice	65:157	Immunological evaluation of a recombinant vaccine delivered with an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice.					
37003502	4	75	theme	ROP18	644:648	arg1	proteins					650:657	ROP18 proteins	644:657	ROP18 proteins	644:657	METHODS The recombinant MIC6 and ROP18 proteins were obtained by affinity chromatography and loaded onto AHACNP-HG by magnetic stirring.					
37003502	1	76	theme	public	229:234	arg1	health					236:241	the public health	225:241	the public health	225:241	BACKGROUND Toxoplasma gondii (T. gondii) is not only a threat to the public health but it also poses adverse impacts on the livestock industry.					
37003502	11	77	from	properties	1760:1769	arg1	model					1780:1784	mouse model	1774:1784	mouse model	1774:1784	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
37003502	4	78	dep	METHODS	611:617	arg1	proteins					650:657	ROP18 proteins	644:657	ROP18 proteins	644:657	METHODS The recombinant MIC6 and ROP18 proteins were obtained by affinity chromatography and loaded onto AHACNP-HG by magnetic stirring.					
37003502	4	78	dep	METHODS	611:617	arg1	MIC6					635:638	The recombinant MIC6	619:638	The recombinant MIC6	619:638	METHODS The recombinant MIC6 and ROP18 proteins were obtained by affinity chromatography and loaded onto AHACNP-HG by magnetic stirring.					
37003502	5	79	theme	zeta	871:874	arg1	potential					876:884	zeta potential	871:884	zeta potential	871:884	The characterizations of AHACNP-HG were investigated, including its structure, rheological property, nanoparticle size and zeta potential, its ability to release protein in vitro and toxicology in vivo.					
37003502	11	80	dep	T.	1668:1669	arg1	gondii					1671:1676	gondii	1671:1676	gondii	1671:1676	CONCLUSION Our data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.					
35961168	0	0	theme	short-chain	78:88	arg1	acids					96:100	short-chain fatty acids	78:100	short-chain fatty acids	78:100	Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.					
35961168	3	1	theme	acid	691:694	arg1	production					651:660	the production	647:660	the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid	647:720	Fiber supplementation significantly increased the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid, but decreased the production of oxindole, tryptamine, and serotonin.					
35961168	6	2	theme	colon	1111:1115	arg1	segment					1117:1123	colon segment	1111:1123	colon segment	1111:1123	Overall, these results suggest that fiber supplementation and colon segment affect the composition of gut microbiota and the microbial catabolism of tryptophan.					
35961168	3	3	theme	indole-3-lactic	701:715	arg1	acid					717:720	indole-3-lactic acid	701:720	indole-3-lactic acid	701:720	Fiber supplementation significantly increased the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid, but decreased the production of oxindole, tryptamine, and serotonin.					
35961168	0	4	from	effects	9:15	arg1	acids					96:100	short-chain fatty acids	78:100	short-chain fatty acids	78:100	Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.					
35961168	0	4	from	effects	9:15	arg1	microbiota-derived					47:64	microbiota-derived	47:64	microbiota-derived	47:64	Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.					
35961168	4	5	theme	acid	855:858	arg1	production					825:834	the production	821:834	the production of indole-3-acetic acid and indole-3-aldehyde	821:880	Pectin specifically promoted the production of indole-3-acetic acid and indole-3-aldehyde.					
35961168	1	6	theme	combination	140:150	arg1	Effects					103:109	Effects	103:109	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments	103:264	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	1	7	theme	proximal	334:341	arg1	PC					350:351	PC	350:351	PC	350:351	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	1	7	theme	proximal	334:341	arg1	colon					343:347	proximal colon	334:347	proximal colon (PC)	334:352	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	1	8	theme	Simulator	396:404	arg1	compartments					376:387	proximal colon (PC) and distal colon (DC) compartments	334:387	proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem	334:444	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	5	9	theme	Bacteroidetes	970:982	arg1	abundance					957:965	the relative abundance	944:965	the relative abundance of Bacteroidetes	944:982	Interestingly, supplementation of pectin or inulin increased the relative abundance of Bacteroidetes whereas supplementation of a mixture of two fibers decreased it.					
35961168	6	10	theme	microbiota	1155:1164	arg1	composition					1136:1146	the composition	1132:1146	the composition of gut microbiota	1132:1164	Overall, these results suggest that fiber supplementation and colon segment affect the composition of gut microbiota and the microbial catabolism of tryptophan.					
35961168	6	10	theme	microbiota	1155:1164	arg1	catabolism					1184:1193	the microbial catabolism	1170:1193	the microbial catabolism of tryptophan	1170:1207	Overall, these results suggest that fiber supplementation and colon segment affect the composition of gut microbiota and the microbial catabolism of tryptophan.					
35961168	1	11	theme	different	241:249	arg1	segments					257:264	different colon segments	241:264	different colon segments	241:264	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	0	12	theme	fatty	90:94	arg1	acids					96:100	short-chain fatty acids	78:100	short-chain fatty acids	78:100	Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.					
35961168	1	13	theme	colon	343:347	arg1	compartments					376:387	proximal colon (PC) and distal colon (DC) compartments	334:387	proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem	334:444	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	4	14	theme	indole-3-aldehyde	864:880	arg1	production					825:834	the production	821:834	the production of indole-3-acetic acid and indole-3-aldehyde	821:880	Pectin specifically promoted the production of indole-3-acetic acid and indole-3-aldehyde.					
35961168	6	15	theme	tryptophan	1198:1207	arg1	composition					1136:1146	the composition	1132:1146	the composition of gut microbiota	1132:1164	Overall, these results suggest that fiber supplementation and colon segment affect the composition of gut microbiota and the microbial catabolism of tryptophan.					
35961168	6	15	theme	tryptophan	1198:1207	arg1	catabolism					1184:1193	the microbial catabolism	1170:1193	the microbial catabolism of tryptophan	1170:1207	Overall, these results suggest that fiber supplementation and colon segment affect the composition of gut microbiota and the microbial catabolism of tryptophan.					
35961168	1	16	theme	colon	251:255	arg1	segments					257:264	different colon segments	241:264	different colon segments	241:264	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	3	17	theme	acid	717:720	arg1	production					651:660	the production	647:660	the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid	647:720	Fiber supplementation significantly increased the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid, but decreased the production of oxindole, tryptamine, and serotonin.					
35961168	1	18	theme	Human	409:413	arg1	Ecosystem					436:444	Human Intestinal Microbial Ecosystem	409:444	Human Intestinal Microbial Ecosystem	409:444	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	2	19	from	compartment	464:474	arg1	Bacteria					447:454	Bacteria	447:454	Bacteria from DC compartment	447:474	Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment.					
35961168	2	20	theme	PC	585:586	arg1	compartment					588:598	PC compartment	585:598	PC compartment	585:598	Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment.					
35961168	1	21	theme	Intestinal	415:424	arg1	Ecosystem					436:444	Human Intestinal Microbial Ecosystem	409:444	Human Intestinal Microbial Ecosystem	409:444	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	3	22	theme	serotonin	781:789	arg1	production					741:750	the production	737:750	the production of oxindole, tryptamine, and serotonin	737:789	Fiber supplementation significantly increased the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid, but decreased the production of oxindole, tryptamine, and serotonin.					
35961168	6	23	theme	fiber	1085:1089	arg1	supplementation					1091:1105	fiber supplementation	1085:1105	fiber supplementation	1085:1105	Overall, these results suggest that fiber supplementation and colon segment affect the composition of gut microbiota and the microbial catabolism of tryptophan.					
35961168	1	24	from	segments	257:264	arg1	production					159:168	the production	155:168	the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments	155:264	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	1	25	theme	Microbial	426:434	arg1	Ecosystem					436:444	Human Intestinal Microbial Ecosystem	409:444	Human Intestinal Microbial Ecosystem	409:444	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	6	26	theme	microbial	1174:1182	arg1	catabolism					1184:1193	the microbial catabolism	1170:1193	the microbial catabolism of tryptophan	1170:1207	Overall, these results suggest that fiber supplementation and colon segment affect the composition of gut microbiota and the microbial catabolism of tryptophan.					
35961168	0	27	theme	Distinct	0:7	arg1	effects					9:15	Distinct effects	0:15	Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.	0:101	Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.					
35961168	1	28	theme	Ecosystem	436:444	arg1	Simulator					396:404	the Simulator	392:404	the Simulator of Human Intestinal Microbial Ecosystem	392:444	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	5	29	theme	relative	948:955	arg1	abundance					957:965	the relative abundance	944:965	the relative abundance of Bacteroidetes	944:982	Interestingly, supplementation of pectin or inulin increased the relative abundance of Bacteroidetes whereas supplementation of a mixture of two fibers decreased it.					
35961168	0	30	theme	fiber	20:24	arg1	effects					9:15	Distinct effects	0:15	Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.	0:101	Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.					
35961168	1	31	theme	microbiota-derived	173:190	arg1	production					159:168	the production	155:168	the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments	155:264	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	4	32	theme	indole-3-acetic	839:853	arg1	acid					855:858	indole-3-acetic acid	839:858	indole-3-acetic acid	839:858	Pectin specifically promoted the production of indole-3-acetic acid and indole-3-aldehyde.					
35961168	0	33	dep	microbiota-derived	47:64	arg1	indoles					66:72	indoles	66:72	indoles	66:72	Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.					
35961168	0	34	theme	colon	30:34	arg1	effects					9:15	Distinct effects	0:15	Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.	0:101	Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.					
35961168	2	35	theme	Firmicutes	502:511	arg1	capacity					528:535	a stronger capacity	517:535	a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment	517:598	Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment.					
35961168	2	35	theme	Firmicutes	502:511	arg1	abundance					489:497	a higher abundance	480:497	a higher abundance of Firmicutes	480:511	Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment.					
35961168	1	36	theme	distal	358:363	arg1	colon					365:369	distal colon	358:369	distal colon (DC)	358:374	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	1	36	theme	distal	358:363	arg1	DC					372:373	DC	372:373	DC	372:373	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	5	37	theme	fibers	1028:1033	arg1	mixture					1013:1019	a mixture	1011:1019	a mixture of two fibers	1011:1033	Interestingly, supplementation of pectin or inulin increased the relative abundance of Bacteroidetes whereas supplementation of a mixture of two fibers decreased it.					
35961168	3	38	theme	oxindole	755:762	arg1	production					741:750	the production	737:750	the production of oxindole, tryptamine, and serotonin	737:789	Fiber supplementation significantly increased the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid, but decreased the production of oxindole, tryptamine, and serotonin.					
35961168	1	39	theme	colon	365:369	arg1	compartments					376:387	proximal colon (PC) and distal colon (DC) compartments	334:387	proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem	334:444	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	1	40	theme	short-chain	204:214	arg1	SCFAs					229:233	SCFAs	229:233	SCFAs	229:233	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	1	40	theme	short-chain	204:214	arg1	acids					222:226	short-chain fatty acids	204:226	short-chain fatty acids (SCFAs)	204:234	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	1	41	theme	batch	289:293	arg1	system					295:300	a batch system	287:300	a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem	287:444	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	3	42	theme	Fiber	601:605	arg1	supplementation					607:621	Fiber supplementation	601:621	Fiber supplementation	601:621	Fiber supplementation significantly increased the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid, but decreased the production of oxindole, tryptamine, and serotonin.					
35961168	5	43	theme	pectin	917:922	arg1	supplementation					898:912	supplementation	898:912	supplementation of pectin or inulin	898:932	Interestingly, supplementation of pectin or inulin increased the relative abundance of Bacteroidetes whereas supplementation of a mixture of two fibers decreased it.					
35961168	2	44	from	compartment	588:598	arg1	bacteria					571:578	bacteria	571:578	bacteria from PC compartment	571:598	Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment.					
35961168	1	45	theme	pectin	114:119	arg1	Effects					103:109	Effects	103:109	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments	103:264	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	3	46	theme	SCFAs	665:669	arg1	production					651:660	the production	647:660	the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid	647:720	Fiber supplementation significantly increased the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid, but decreased the production of oxindole, tryptamine, and serotonin.					
35961168	0	47	dep	fiber	20:24	arg1	segment					36:42	segment	36:42	segment	36:42	Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.					
35961168	5	48	theme	inulin	927:932	arg1	supplementation					898:912	supplementation	898:912	supplementation of pectin or inulin	898:932	Interestingly, supplementation of pectin or inulin increased the relative abundance of Bacteroidetes whereas supplementation of a mixture of two fibers decreased it.					
35961168	1	49	from	Effects	103:109	arg1	production					159:168	the production	155:168	the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments	155:264	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	1	50	theme	fatty	216:220	arg1	SCFAs					229:233	SCFAs	229:233	SCFAs	229:233	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	1	50	theme	fatty	216:220	arg1	acids					222:226	short-chain fatty acids	204:226	short-chain fatty acids (SCFAs)	204:234	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	2	51	theme	stronger	519:526	arg1	capacity					528:535	a stronger capacity	517:535	a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment	517:598	Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment.					
35961168	5	52	theme	mixture	1013:1019	arg1	supplementation					992:1006	supplementation	992:1006	supplementation of a mixture of two fibers	992:1033	Interestingly, supplementation of pectin or inulin increased the relative abundance of Bacteroidetes whereas supplementation of a mixture of two fibers decreased it.					
35961168	2	53	contain	had	476:478	arg2	abundance					489:497	a higher abundance	480:497	a higher abundance of Firmicutes	480:511	Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment.					
35961168	2	53	contain	had	476:478	arg1	Bacteria					447:454	Bacteria	447:454	Bacteria from DC compartment	447:474	Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment.					
35961168	2	53	contain	had	476:478	arg2	capacity					528:535	a stronger capacity	517:535	a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment	517:598	Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment.					
35961168	1	54	theme	inulin	122:127	arg1	Effects					103:109	Effects	103:109	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments	103:264	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	6	55	theme	gut	1151:1153	arg1	microbiota					1155:1164	gut microbiota	1151:1164	gut microbiota	1151:1164	Overall, these results suggest that fiber supplementation and colon segment affect the composition of gut microbiota and the microbial catabolism of tryptophan.					
35961168	1	56	theme	acids	222:226	arg1	production					159:168	the production	155:168	the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments	155:264	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	1	57	from	compartments	376:387	arg1	microbiota					318:327	microbiota	318:327	microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem	318:444	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	1	58	dep	microbiota-derived	173:190	arg1	indoles					192:198	indoles	192:198	indoles	192:198	Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem.					
35961168	3	59	theme	tryptamine	765:774	arg1	production					741:750	the production	737:750	the production of oxindole, tryptamine, and serotonin	737:789	Fiber supplementation significantly increased the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid, but decreased the production of oxindole, tryptamine, and serotonin.					
35961168	2	60	theme	DC	461:462	arg1	compartment					464:474	DC compartment	461:474	DC compartment	461:474	Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment.					
35961168	3	61	theme	indole-3-propionic	672:689	arg1	acid					691:694	indole-3-propionic acid	672:694	indole-3-propionic acid	672:694	Fiber supplementation significantly increased the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid, but decreased the production of oxindole, tryptamine, and serotonin.					
35961168	2	62	theme	higher	482:487	arg1	abundance					489:497	a higher abundance	480:497	a higher abundance of Firmicutes	480:511	Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment.					
35122572	8	0	theme	nutrient	1122:1129	arg1	ratio					1131:1135	This nutrient ratio	1117:1135	This nutrient ratio	1117:1135	This nutrient ratio also maximised male reproductive rates, but females required more protein to maximise daily fecundity (1P:1.22C).					
35122572	0	1	from	differences	12:22	arg1	effects					31:37	the effects	27:37	the effects of protein	27:48	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	0	1	from	differences	12:22	arg1	lifespan					71:78	lifespan	71:78	lifespan	71:78	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	0	1	from	differences	12:22	arg1	reproduction					84:95	reproduction	84:95	reproduction	84:95	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	7	2	theme	male	1023:1026	arg1	lifespan					1039:1046	male and female lifespan	1023:1046	male and female lifespan	1023:1046	We find that male and female lifespan was maximised on low protein, high carbohydrate blends (~ 1P:15.9C).					
35122572	12	3	contain	has	1809:1811	arg2	advantage					1817:1825	the advantage	1813:1825	the advantage of creating nutritional landscapes	1813:1860	The most appropriate method will depend on the question and species being studied, but the approach adopted here has the advantage of creating nutritional landscapes when dietary intake is hard to quantify.					
35122572	12	3	contain	has	1809:1811	arg1	approach					1787:1794	the approach	1783:1794	the approach adopted here	1783:1807	The most appropriate method will depend on the question and species being studied, but the approach adopted here has the advantage of creating nutritional landscapes when dietary intake is hard to quantify.					
35122572	9	4	with	consistent	1269:1278	arg1	outcomes					1296:1303	CAFE assay outcomes	1285:1303	CAFE assay outcomes	1285:1303	These results are consistent with CAFE assay outcomes.					
35122572	1	5	theme	evolutionary	242:253	arg1	biology					255:261	evolutionary biology	242:261	evolutionary biology	242:261	Understanding how diet affects reproduction and survival is a central aim in evolutionary biology.					
35122572	10	6	theme	experimental	1496:1507	arg1	diets					1509:1513	experimental diets	1496:1513	experimental diets	1496:1513	However, the approach employed here improved female fitness relative to CAFE assays, while effects of agar versus CAFE feeding on male fitness traits depended on the nutrient composition of experimental diets.					
35122572	5	7	from	protein	770:776	arg1	D.					822:823	D.	822:823	D.	822:823	Here, we use the Geometric Framework of Nutrition to create nutrient landscapes that map sex-specific relationships between protein, carbohydrate, lifespan and reproduction in D. melanogaster.					
35122572	10	8	theme	agar	1408:1411	arg1	feeding					1425:1431	agar versus CAFE feeding	1408:1431	feeding	1425:1431	However, the approach employed here improved female fitness relative to CAFE assays, while effects of agar versus CAFE feeding on male fitness traits depended on the nutrient composition of experimental diets.					
35122572	7	9	theme	carbohydrate	1083:1094	arg1	blends					1096:1101	high carbohydrate blends	1078:1101	high carbohydrate blends (~ 1P:15.9C)	1078:1114	We find that male and female lifespan was maximised on low protein, high carbohydrate blends (~ 1P:15.9C).					
35122572	7	9	theme	carbohydrate	1083:1094	arg1	protein					1069:1075	low protein	1065:1075	low protein	1065:1075	We find that male and female lifespan was maximised on low protein, high carbohydrate blends (~ 1P:15.9C).					
35122572	7	9	theme	carbohydrate	1083:1094	arg1	~ 1P:15.9C					1104:1113	~ 1P:15.9C	1104:1113	~ 1P:15.9C	1104:1113	We find that male and female lifespan was maximised on low protein, high carbohydrate blends (~ 1P:15.9C).					
35122572	5	10	theme	nutrient	706:713	arg1	landscapes					715:724	nutrient landscapes	706:724	nutrient landscapes that map sex-specific relationships between protein, carbohydrate, lifespan and reproduction in D. melanogaster	706:836	Here, we use the Geometric Framework of Nutrition to create nutrient landscapes that map sex-specific relationships between protein, carbohydrate, lifespan and reproduction in D. melanogaster.					
35122572	5	11	from	reproduction	806:817	arg1	D.					822:823	D.	822:823	D.	822:823	Here, we use the Geometric Framework of Nutrition to create nutrient landscapes that map sex-specific relationships between protein, carbohydrate, lifespan and reproduction in D. melanogaster.					
35122572	4	12	theme	D.	565:566	arg1	survival					581:588	D. melanogaster survival	565:588	D. melanogaster survival	565:588	However, CAFE feeding reduces D. melanogaster survival and reproduction, so may distort diet-fitness outcomes.					
35122572	8	13	theme	male	1152:1155	arg1	rates					1170:1174	male reproductive rates	1152:1174	male reproductive rates	1152:1174	This nutrient ratio also maximised male reproductive rates, but females required more protein to maximise daily fecundity (1P:1.22C).					
35122572	9	14	theme	CAFE	1285:1288	arg1	outcomes					1296:1303	CAFE assay outcomes	1285:1303	CAFE assay outcomes	1285:1303	These results are consistent with CAFE assay outcomes.					
35122572	10	15	theme	male	1436:1439	arg1	traits					1449:1454	male fitness traits	1436:1454	male fitness traits	1436:1454	However, the approach employed here improved female fitness relative to CAFE assays, while effects of agar versus CAFE feeding on male fitness traits depended on the nutrient composition of experimental diets.					
35122572	10	16	theme	female	1351:1356	arg1	fitness					1358:1364	female fitness	1351:1364	female fitness	1351:1364	However, the approach employed here improved female fitness relative to CAFE assays, while effects of agar versus CAFE feeding on male fitness traits depended on the nutrient composition of experimental diets.					
35122572	2	17	theme	reproductive	365:376	arg1	traits					378:383	male reproductive traits	360:383	male reproductive traits	360:383	Although this relationship is likely to differ between the sexes, we lack data relating diet to male reproductive traits.					
35122572	6	18	theme	broad	976:980	arg1	coverage					982:989	broad coverage	976:989	broad coverage of nutrient space	976:1007	Rather than creating landscapes with consumption data, we map traits onto the nutrient composition of forty agar-based diets, generating broad coverage of nutrient space.					
35122572	5	19	from	lifespan	793:800	arg1	D.					822:823	D.	822:823	D.	822:823	Here, we use the Geometric Framework of Nutrition to create nutrient landscapes that map sex-specific relationships between protein, carbohydrate, lifespan and reproduction in D. melanogaster.					
35122572	6	20	theme	nutrient	994:1001	arg1	space					1003:1007	nutrient space	994:1007	nutrient space	994:1007	Rather than creating landscapes with consumption data, we map traits onto the nutrient composition of forty agar-based diets, generating broad coverage of nutrient space.					
35122572	11	21	theme	informative	1532:1542	arg1	landscapes					1553:1562	informative nutrient landscapes	1532:1562	informative nutrient landscapes	1532:1562	We suggest that informative nutrient landscapes can be made without measuring individual nutrient intake and that in many cases, this may be preferable to using the CAFE approach.					
35122572	3	22	theme	CAFE	521:524	arg1	method					527:532	the CApillary FEeder (CAFE) method	499:532	the CApillary FEeder (CAFE) method	499:532	One exception to this general pattern is Drosophila melanogaster, where male dietary intake was quantified using the CApillary FEeder (CAFE) method.					
35122572	11	23	from	preferable	1657:1666	arg1	cases					1638:1642	many cases	1633:1642	many cases	1633:1642	We suggest that informative nutrient landscapes can be made without measuring individual nutrient intake and that in many cases, this may be preferable to using the CAFE approach.					
35122572	0	24	theme	sex	8:10	arg1	differences					12:22	Mapping sex differences	0:22	Mapping sex differences in the effects of protein	0:48	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	6	25	theme	consumption	876:886	arg1	data					888:891	consumption data	876:891	consumption data	876:891	Rather than creating landscapes with consumption data, we map traits onto the nutrient composition of forty agar-based diets, generating broad coverage of nutrient space.					
35122572	7	26	theme	low	1065:1067	arg1	blends					1096:1101	high carbohydrate blends	1078:1101	high carbohydrate blends (~ 1P:15.9C)	1078:1114	We find that male and female lifespan was maximised on low protein, high carbohydrate blends (~ 1P:15.9C).					
35122572	7	26	theme	low	1065:1067	arg1	protein					1069:1075	low protein	1065:1075	low protein	1065:1075	We find that male and female lifespan was maximised on low protein, high carbohydrate blends (~ 1P:15.9C).					
35122572	11	27	theme	nutrient	1544:1551	arg1	landscapes					1553:1562	informative nutrient landscapes	1532:1562	informative nutrient landscapes	1532:1562	We suggest that informative nutrient landscapes can be made without measuring individual nutrient intake and that in many cases, this may be preferable to using the CAFE approach.					
35122572	8	28	theme	daily	1223:1227	arg1	1P:1.22C					1240:1247	1P:1.22C	1240:1247	1P:1.22C	1240:1247	This nutrient ratio also maximised male reproductive rates, but females required more protein to maximise daily fecundity (1P:1.22C).					
35122572	8	28	theme	daily	1223:1227	arg1	fecundity					1229:1237	daily fecundity	1223:1237	daily fecundity (1P:1.22C)	1223:1248	This nutrient ratio also maximised male reproductive rates, but females required more protein to maximise daily fecundity (1P:1.22C).					
35122572	7	29	theme	female	1032:1037	arg1	lifespan					1039:1046	male and female lifespan	1023:1046	male and female lifespan	1023:1046	We find that male and female lifespan was maximised on low protein, high carbohydrate blends (~ 1P:15.9C).					
35122572	3	30	theme	general	408:414	arg1	pattern					416:422	this general pattern	403:422	this general pattern	403:422	One exception to this general pattern is Drosophila melanogaster, where male dietary intake was quantified using the CApillary FEeder (CAFE) method.					
35122572	0	31	theme	Mapping	0:6	arg1	differences					12:22	Mapping sex differences	0:22	Mapping sex differences in the effects of protein	0:48	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	10	32	theme	CAFE	1420:1423	arg1	feeding					1425:1431	agar versus CAFE feeding	1408:1431	feeding	1425:1431	However, the approach employed here improved female fitness relative to CAFE assays, while effects of agar versus CAFE feeding on male fitness traits depended on the nutrient composition of experimental diets.					
35122572	12	33	theme	appropriate	1705:1715	arg1	method					1717:1722	The most appropriate method	1696:1722	The most appropriate method	1696:1722	The most appropriate method will depend on the question and species being studied, but the approach adopted here has the advantage of creating nutritional landscapes when dietary intake is hard to quantify.					
35122572	11	34	theme	CAFE	1681:1684	arg1	approach					1686:1693	the CAFE approach	1677:1693	the CAFE approach	1677:1693	We suggest that informative nutrient landscapes can be made without measuring individual nutrient intake and that in many cases, this may be preferable to using the CAFE approach.					
35122572	2	35	theme	male	360:363	arg1	traits					378:383	male reproductive traits	360:383	male reproductive traits	360:383	Although this relationship is likely to differ between the sexes, we lack data relating diet to male reproductive traits.					
35122572	12	36	theme	dietary	1867:1873	arg1	intake					1875:1880	dietary intake	1867:1880	dietary intake	1867:1880	The most appropriate method will depend on the question and species being studied, but the approach adopted here has the advantage of creating nutritional landscapes when dietary intake is hard to quantify.					
35122572	10	37	from	effects	1397:1403	arg1	traits					1449:1454	male fitness traits	1436:1454	male fitness traits	1436:1454	However, the approach employed here improved female fitness relative to CAFE assays, while effects of agar versus CAFE feeding on male fitness traits depended on the nutrient composition of experimental diets.					
35122572	6	38	theme	diets	958:962	arg1	composition					926:936	the nutrient composition	913:936	the nutrient composition of forty agar-based diets	913:962	Rather than creating landscapes with consumption data, we map traits onto the nutrient composition of forty agar-based diets, generating broad coverage of nutrient space.					
35122572	10	39	theme	CAFE	1378:1381	arg1	assays					1383:1388	CAFE assays	1378:1388	CAFE assays	1378:1388	However, the approach employed here improved female fitness relative to CAFE assays, while effects of agar versus CAFE feeding on male fitness traits depended on the nutrient composition of experimental diets.					
35122572	4	40	theme	CAFE	544:547	arg1	feeding					549:555	CAFE feeding	544:555	CAFE feeding	544:555	However, CAFE feeding reduces D. melanogaster survival and reproduction, so may distort diet-fitness outcomes.					
35122572	0	41	from	lifespan	71:78	arg1	melanogaster					111:122	Drosophila melanogaster	100:122	Drosophila melanogaster	100:122	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	3	42	theme	male	458:461	arg1	intake					471:476	male dietary intake	458:476	male dietary intake	458:476	One exception to this general pattern is Drosophila melanogaster, where male dietary intake was quantified using the CApillary FEeder (CAFE) method.					
35122572	6	43	theme	agar-based	947:956	arg1	diets					958:962	forty agar-based diets	941:962	forty agar-based diets	941:962	Rather than creating landscapes with consumption data, we map traits onto the nutrient composition of forty agar-based diets, generating broad coverage of nutrient space.					
35122572	5	44	theme	Nutrition	686:694	arg1	Framework					673:681	the Geometric Framework	659:681	the Geometric Framework of Nutrition	659:694	Here, we use the Geometric Framework of Nutrition to create nutrient landscapes that map sex-specific relationships between protein, carbohydrate, lifespan and reproduction in D. melanogaster.					
35122572	4	45	dep	D.	565:566	arg1	melanogaster					568:579	D. melanogaster	565:579	D. melanogaster survival	565:588	However, CAFE feeding reduces D. melanogaster survival and reproduction, so may distort diet-fitness outcomes.					
35122572	6	46	with	landscapes	860:869	arg1	data					888:891	consumption data	876:891	consumption data	876:891	Rather than creating landscapes with consumption data, we map traits onto the nutrient composition of forty agar-based diets, generating broad coverage of nutrient space.					
35122572	4	47	theme	diet-fitness	623:634	arg1	outcomes					636:643	diet-fitness outcomes	623:643	diet-fitness outcomes	623:643	However, CAFE feeding reduces D. melanogaster survival and reproduction, so may distort diet-fitness outcomes.					
35122572	12	48	theme	nutritional	1839:1849	arg1	landscapes					1851:1860	nutritional landscapes	1839:1860	nutritional landscapes	1839:1860	The most appropriate method will depend on the question and species being studied, but the approach adopted here has the advantage of creating nutritional landscapes when dietary intake is hard to quantify.					
35122572	10	49	theme	fitness	1441:1447	arg1	traits					1449:1454	male fitness traits	1436:1454	male fitness traits	1436:1454	However, the approach employed here improved female fitness relative to CAFE assays, while effects of agar versus CAFE feeding on male fitness traits depended on the nutrient composition of experimental diets.					
35122572	0	50	from	carbohydrates	54:66	arg1	effects					31:37	the effects	27:37	the effects of protein	27:48	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	0	50	from	carbohydrates	54:66	arg1	lifespan					71:78	lifespan	71:78	lifespan	71:78	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	0	50	from	carbohydrates	54:66	arg1	reproduction					84:95	reproduction	84:95	reproduction	84:95	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	8	51	theme	reproductive	1157:1168	arg1	rates					1170:1174	male reproductive rates	1152:1174	male reproductive rates	1152:1174	This nutrient ratio also maximised male reproductive rates, but females required more protein to maximise daily fecundity (1P:1.22C).					
35122572	0	52	theme	protein	42:48	arg1	effects					31:37	the effects	27:37	the effects of protein	27:48	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	10	53	theme	feeding	1425:1431	arg1	effects					1397:1403	effects	1397:1403	effects of agar versus CAFE feeding on male fitness traits	1397:1454	However, the approach employed here improved female fitness relative to CAFE assays, while effects of agar versus CAFE feeding on male fitness traits depended on the nutrient composition of experimental diets.					
35122572	1	54	from	aim	235:237	arg1	biology					255:261	evolutionary biology	242:261	evolutionary biology	242:261	Understanding how diet affects reproduction and survival is a central aim in evolutionary biology.					
35122572	1	55	dep	Understanding	165:177	arg1	affects					188:194	affects	188:194	affects reproduction and survival	188:220	Understanding how diet affects reproduction and survival is a central aim in evolutionary biology.					
35122572	9	56	theme	assay	1290:1294	arg1	outcomes					1296:1303	CAFE assay outcomes	1285:1303	CAFE assay outcomes	1285:1303	These results are consistent with CAFE assay outcomes.					
35122572	0	57	theme	nutrient	138:145	arg1	intake					147:152	nutrient intake	138:152	nutrient intake	138:152	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	10	58	theme	diets	1509:1513	arg1	composition					1481:1491	the nutrient composition	1468:1491	the nutrient composition of experimental diets	1468:1513	However, the approach employed here improved female fitness relative to CAFE assays, while effects of agar versus CAFE feeding on male fitness traits depended on the nutrient composition of experimental diets.					
35122572	7	59	theme	high	1078:1081	arg1	blends					1096:1101	high carbohydrate blends	1078:1101	high carbohydrate blends (~ 1P:15.9C)	1078:1114	We find that male and female lifespan was maximised on low protein, high carbohydrate blends (~ 1P:15.9C).					
35122572	7	59	theme	high	1078:1081	arg1	protein					1069:1075	low protein	1065:1075	low protein	1065:1075	We find that male and female lifespan was maximised on low protein, high carbohydrate blends (~ 1P:15.9C).					
35122572	7	59	theme	high	1078:1081	arg1	~ 1P:15.9C					1104:1113	~ 1P:15.9C	1104:1113	~ 1P:15.9C	1104:1113	We find that male and female lifespan was maximised on low protein, high carbohydrate blends (~ 1P:15.9C).					
35122572	11	60	from	cases	1638:1642	arg1	preferable					1657:1666	preferable	1657:1666	preferable	1657:1666	We suggest that informative nutrient landscapes can be made without measuring individual nutrient intake and that in many cases, this may be preferable to using the CAFE approach.					
35122572	6	61	theme	nutrient	917:924	arg1	composition					926:936	the nutrient composition	913:936	the nutrient composition of forty agar-based diets	913:962	Rather than creating landscapes with consumption data, we map traits onto the nutrient composition of forty agar-based diets, generating broad coverage of nutrient space.					
35122572	10	62	theme	nutrient	1472:1479	arg1	composition					1481:1491	the nutrient composition	1468:1491	the nutrient composition of experimental diets	1468:1513	However, the approach employed here improved female fitness relative to CAFE assays, while effects of agar versus CAFE feeding on male fitness traits depended on the nutrient composition of experimental diets.					
35122572	3	63	theme	dietary	463:469	arg1	intake					471:476	male dietary intake	458:476	male dietary intake	458:476	One exception to this general pattern is Drosophila melanogaster, where male dietary intake was quantified using the CApillary FEeder (CAFE) method.					
35122572	11	64	theme	many	1633:1636	arg1	cases					1638:1642	many cases	1633:1642	many cases	1633:1642	We suggest that informative nutrient landscapes can be made without measuring individual nutrient intake and that in many cases, this may be preferable to using the CAFE approach.					
35122572	5	65	theme	Geometric	663:671	arg1	Framework					673:681	the Geometric Framework	659:681	the Geometric Framework of Nutrition	659:694	Here, we use the Geometric Framework of Nutrition to create nutrient landscapes that map sex-specific relationships between protein, carbohydrate, lifespan and reproduction in D. melanogaster.					
35122572	0	66	from	reproduction	84:95	arg1	melanogaster					111:122	Drosophila melanogaster	100:122	Drosophila melanogaster	100:122	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	0	67	dep	differences	12:22	arg1	measuring					128:136	measuring	128:136	is measuring nutrient intake essential	125:162	Mapping sex differences in the effects of protein and carbohydrates on lifespan and reproduction in Drosophila melanogaster: is measuring nutrient intake essential?					
35122572	11	68	theme	individual	1594:1603	arg1	intake					1614:1619	individual nutrient intake	1594:1619	individual nutrient intake	1594:1619	We suggest that informative nutrient landscapes can be made without measuring individual nutrient intake and that in many cases, this may be preferable to using the CAFE approach.					
35122572	1	69	theme	central	227:233	arg1	aim					235:237	a central aim	225:237	a central aim in evolutionary biology	225:261	Understanding how diet affects reproduction and survival is a central aim in evolutionary biology.					
35122572	1	69	theme	central	227:233	arg1	Understanding					165:177	Understanding	165:177	Understanding how diet affects reproduction and survival	165:220	Understanding how diet affects reproduction and survival is a central aim in evolutionary biology.					
35122572	5	70	dep	D.	822:823	arg1	melanogaster					825:836	D. melanogaster	822:836	D. melanogaster	822:836	Here, we use the Geometric Framework of Nutrition to create nutrient landscapes that map sex-specific relationships between protein, carbohydrate, lifespan and reproduction in D. melanogaster.					
35122572	11	71	theme	nutrient	1605:1612	arg1	intake					1614:1619	individual nutrient intake	1594:1619	individual nutrient intake	1594:1619	We suggest that informative nutrient landscapes can be made without measuring individual nutrient intake and that in many cases, this may be preferable to using the CAFE approach.					
35122572	3	72	theme	CApillary	503:511	arg1	method					527:532	the CApillary FEeder (CAFE) method	499:532	the CApillary FEeder (CAFE) method	499:532	One exception to this general pattern is Drosophila melanogaster, where male dietary intake was quantified using the CApillary FEeder (CAFE) method.					
35122572	5	73	theme	sex-specific	735:746	arg1	relationships					748:760	sex-specific relationships	735:760	sex-specific relationships between protein, carbohydrate, lifespan and reproduction in D. melanogaster	735:836	Here, we use the Geometric Framework of Nutrition to create nutrient landscapes that map sex-specific relationships between protein, carbohydrate, lifespan and reproduction in D. melanogaster.					
35122572	5	74	from	carbohydrate	779:790	arg1	D.					822:823	D.	822:823	D.	822:823	Here, we use the Geometric Framework of Nutrition to create nutrient landscapes that map sex-specific relationships between protein, carbohydrate, lifespan and reproduction in D. melanogaster.					
35122572	3	75	theme	FEeder	513:518	arg1	method					527:532	the CApillary FEeder (CAFE) method	499:532	the CApillary FEeder (CAFE) method	499:532	One exception to this general pattern is Drosophila melanogaster, where male dietary intake was quantified using the CApillary FEeder (CAFE) method.					
35122572	6	76	theme	space	1003:1007	arg1	coverage					982:989	broad coverage	976:989	broad coverage of nutrient space	976:1007	Rather than creating landscapes with consumption data, we map traits onto the nutrient composition of forty agar-based diets, generating broad coverage of nutrient space.					
35065837	3	0	theme	mycobiota	319:327	arg1	composition					329:339	mycobiota composition	319:339	mycobiota composition	319:339	In this work we study pectin modifications and microbiota and mycobiota composition in strawberry in conventional and organic cultivation systems.					
35065837	5	1	theme	noticeable	713:722	arg1	reorganization					724:737	a more noticeable reorganization	706:737	a more noticeable reorganization of molecular structure	706:760	Pectin molecules extracted from organic strawberries were longer and more branched compared to the conventional strawberries; however a more noticeable reorganization of molecular structure occurred.					
35065837	5	2	theme	conventional	671:682	arg1	strawberries					684:695	the conventional strawberries	667:695	the conventional strawberries	667:695	Pectin molecules extracted from organic strawberries were longer and more branched compared to the conventional strawberries; however a more noticeable reorganization of molecular structure occurred.					
35065837	6	3	theme	molecular	849:857	arg1	structure					859:867	the molecular structure	845:867	the molecular structure of pectin fractions	845:887	The sequential action of the pectinolytic enzymes had a direct effect on the molecular structure of pectin fractions.					
35065837	4	4	theme	strawberry	471:480	arg1	types					462:466	both types	457:466	both types of strawberry	457:480	The enzymatic activity during postharvest storage of both types of strawberry was divided at the fifth day of storage into two phases: postharvest changes and rotting.					
35065837	0	5	from	Changes	0:6	arg1	fruit					78:82	strawberry fruit	67:82	strawberry fruit	67:82	Changes of pectin structure and microbial community composition in strawberry fruit (Fragaria × ananassa Duch.)					
35065837	4	6	theme	enzymatic	408:416	arg1	activity					418:425	The enzymatic activity	404:425	The enzymatic activity during postharvest storage of both types of strawberry	404:480	The enzymatic activity during postharvest storage of both types of strawberry was divided at the fifth day of storage into two phases: postharvest changes and rotting.					
35065837	7	7	theme	pectin	914:919	arg1	structure					921:929	pectin structure	914:929	pectin structure	914:929	The observed changes in pectin structure relate to the synergistic activity of pectinolytic enzymes and some microorganisms.					
35065837	3	8	theme	organic	375:381	arg1	systems					395:401	conventional and organic cultivation systems	358:401	conventional and organic cultivation systems	358:401	In this work we study pectin modifications and microbiota and mycobiota composition in strawberry in conventional and organic cultivation systems.					
35065837	2	9	theme	perishable	152:161	arg1	fruit					163:167	very perishable fruit	147:167	very perishable fruit with rapid postharvest loss of quality and high susceptibility to microbial infections	147:254	Strawberry is very perishable fruit with rapid postharvest loss of quality and high susceptibility to microbial infections.					
35065837	4	10	theme	postharvest	434:444	arg1	storage					446:452	postharvest storage	434:452	postharvest storage of both types of strawberry	434:480	The enzymatic activity during postharvest storage of both types of strawberry was divided at the fifth day of storage into two phases: postharvest changes and rotting.					
35065837	7	11	theme	observed	894:901	arg1	changes					903:909	The observed changes	890:909	The observed changes in pectin structure	890:929	The observed changes in pectin structure relate to the synergistic activity of pectinolytic enzymes and some microorganisms.					
35065837	7	12	theme	microorganisms	999:1012	arg1	activity					957:964	the synergistic activity	941:964	the synergistic activity of pectinolytic enzymes and some microorganisms	941:1012	The observed changes in pectin structure relate to the synergistic activity of pectinolytic enzymes and some microorganisms.					
35065837	8	13	dep	greater	1057:1063	arg1	variety					1076:1082	variety	1076:1082	variety	1076:1082	The organic system was characterized by a greater number and variety of bacteria and fungi during storage as compared to the conventional system.					
35065837	8	13	dep	greater	1057:1063	arg1	number					1065:1070	number	1065:1070	number	1065:1070	The organic system was characterized by a greater number and variety of bacteria and fungi during storage as compared to the conventional system.					
35065837	3	14	theme	conventional	358:369	arg1	systems					395:401	conventional and organic cultivation systems	358:401	conventional and organic cultivation systems	358:401	In this work we study pectin modifications and microbiota and mycobiota composition in strawberry in conventional and organic cultivation systems.					
35065837	6	15	theme	direct	828:833	arg1	effect					835:840	a direct effect	826:840	a direct effect	826:840	The sequential action of the pectinolytic enzymes had a direct effect on the molecular structure of pectin fractions.					
35065837	4	16	theme	fifth	501:505	arg1	day					507:509	the fifth day	497:509	the fifth day of storage	497:520	The enzymatic activity during postharvest storage of both types of strawberry was divided at the fifth day of storage into two phases: postharvest changes and rotting.					
35065837	7	17	theme	pectinolytic	969:980	arg1	enzymes					982:988	pectinolytic enzymes	969:988	pectinolytic enzymes	969:988	The observed changes in pectin structure relate to the synergistic activity of pectinolytic enzymes and some microorganisms.					
35065837	7	18	theme	enzymes	982:988	arg1	activity					957:964	the synergistic activity	941:964	the synergistic activity of pectinolytic enzymes and some microorganisms	941:1012	The observed changes in pectin structure relate to the synergistic activity of pectinolytic enzymes and some microorganisms.					
35065837	8	19	theme	organic	1019:1025	arg1	system					1027:1032	The organic system	1015:1032	The organic system	1015:1032	The organic system was characterized by a greater number and variety of bacteria and fungi during storage as compared to the conventional system.					
35065837	3	20	theme	cultivation	383:393	arg1	systems					395:401	conventional and organic cultivation systems	358:401	conventional and organic cultivation systems	358:401	In this work we study pectin modifications and microbiota and mycobiota composition in strawberry in conventional and organic cultivation systems.					
35065837	5	21	theme	organic	604:610	arg1	strawberries					612:623	organic strawberries	604:623	organic strawberries	604:623	Pectin molecules extracted from organic strawberries were longer and more branched compared to the conventional strawberries; however a more noticeable reorganization of molecular structure occurred.					
35065837	0	22	theme	pectin	11:16	arg1	structure					18:26	pectin structure	11:26	pectin structure	11:26	Changes of pectin structure and microbial community composition in strawberry fruit (Fragaria × ananassa Duch.)					
35065837	3	23	theme	pectin	279:284	arg1	modifications					286:298	pectin modifications	279:298	pectin modifications	279:298	In this work we study pectin modifications and microbiota and mycobiota composition in strawberry in conventional and organic cultivation systems.					
35065837	2	24	theme	postharvest	180:190	arg1	loss					192:195	rapid postharvest loss	174:195	rapid postharvest loss of quality and high susceptibility to microbial infections	174:254	Strawberry is very perishable fruit with rapid postharvest loss of quality and high susceptibility to microbial infections.					
35065837	0	25	theme	microbial	32:40	arg1	composition					52:62	microbial community composition	32:62	microbial community composition	32:62	Changes of pectin structure and microbial community composition in strawberry fruit (Fragaria × ananassa Duch.)					
35065837	2	26	with	fruit	163:167	arg1	loss					192:195	rapid postharvest loss	174:195	rapid postharvest loss of quality and high susceptibility to microbial infections	174:254	Strawberry is very perishable fruit with rapid postharvest loss of quality and high susceptibility to microbial infections.					
35065837	5	27	theme	molecular	742:750	arg1	structure					752:760	molecular structure	742:760	molecular structure	742:760	Pectin molecules extracted from organic strawberries were longer and more branched compared to the conventional strawberries; however a more noticeable reorganization of molecular structure occurred.					
35065837	2	28	theme	rapid	174:178	arg1	loss					192:195	rapid postharvest loss	174:195	rapid postharvest loss of quality and high susceptibility to microbial infections	174:254	Strawberry is very perishable fruit with rapid postharvest loss of quality and high susceptibility to microbial infections.					
35065837	2	29	theme	microbial	235:243	arg1	infections					245:254	microbial infections	235:254	microbial infections	235:254	Strawberry is very perishable fruit with rapid postharvest loss of quality and high susceptibility to microbial infections.					
35065837	5	30	theme	structure	752:760	arg1	reorganization					724:737	a more noticeable reorganization	706:737	a more noticeable reorganization of molecular structure	706:760	Pectin molecules extracted from organic strawberries were longer and more branched compared to the conventional strawberries; however a more noticeable reorganization of molecular structure occurred.					
35065837	0	31	theme	structure	18:26	arg1	Duch					105:108	Duch	105:108	Duch	105:108	Changes of pectin structure and microbial community composition in strawberry fruit (Fragaria × ananassa Duch.)					
35065837	0	31	theme	structure	18:26	arg1	Changes					0:6	Changes	0:6	Changes of pectin structure and microbial community composition in strawberry fruit (Fragaria × ananassa Duch.)	0:110	Changes of pectin structure and microbial community composition in strawberry fruit (Fragaria × ananassa Duch.)					
35065837	6	32	theme	pectinolytic	801:812	arg1	enzymes					814:820	the pectinolytic enzymes	797:820	the pectinolytic enzymes	797:820	The sequential action of the pectinolytic enzymes had a direct effect on the molecular structure of pectin fractions.					
35065837	0	33	theme	composition	52:62	arg1	Duch					105:108	Duch	105:108	Duch	105:108	Changes of pectin structure and microbial community composition in strawberry fruit (Fragaria × ananassa Duch.)					
35065837	0	33	theme	composition	52:62	arg1	Changes					0:6	Changes	0:6	Changes of pectin structure and microbial community composition in strawberry fruit (Fragaria × ananassa Duch.)	0:110	Changes of pectin structure and microbial community composition in strawberry fruit (Fragaria × ananassa Duch.)					
35065837	6	34	theme	pectin	872:877	arg1	fractions					879:887	pectin fractions	872:887	pectin fractions	872:887	The sequential action of the pectinolytic enzymes had a direct effect on the molecular structure of pectin fractions.					
35065837	2	35	theme	susceptibility	217:230	arg1	loss					192:195	rapid postharvest loss	174:195	rapid postharvest loss of quality and high susceptibility to microbial infections	174:254	Strawberry is very perishable fruit with rapid postharvest loss of quality and high susceptibility to microbial infections.					
35065837	6	36	theme	enzymes	814:820	arg1	action					787:792	The sequential action	772:792	The sequential action of the pectinolytic enzymes	772:820	The sequential action of the pectinolytic enzymes had a direct effect on the molecular structure of pectin fractions.					
35065837	0	37	theme	community	42:50	arg1	composition					52:62	microbial community composition	32:62	microbial community composition	32:62	Changes of pectin structure and microbial community composition in strawberry fruit (Fragaria × ananassa Duch.)					
35065837	8	38	theme	conventional	1140:1151	arg1	system					1153:1158	the conventional system	1136:1158	the conventional system	1136:1158	The organic system was characterized by a greater number and variety of bacteria and fungi during storage as compared to the conventional system.					
35065837	6	39	theme	fractions	879:887	arg1	structure					859:867	the molecular structure	845:867	the molecular structure of pectin fractions	845:887	The sequential action of the pectinolytic enzymes had a direct effect on the molecular structure of pectin fractions.					
35065837	2	40	theme	high	212:215	arg1	susceptibility					217:230	high susceptibility	212:230	high susceptibility to microbial infections	212:254	Strawberry is very perishable fruit with rapid postharvest loss of quality and high susceptibility to microbial infections.					
35065837	1	41	theme	cold	119:122	arg1	storage					124:130	cold storage	119:130	cold storage	119:130	during cold storage.					
35065837	7	42	theme	synergistic	945:955	arg1	activity					957:964	the synergistic activity	941:964	the synergistic activity of pectinolytic enzymes and some microorganisms	941:1012	The observed changes in pectin structure relate to the synergistic activity of pectinolytic enzymes and some microorganisms.					
35065837	4	43	theme	postharvest	539:549	arg1	changes					551:557	postharvest changes	539:557	postharvest changes	539:557	The enzymatic activity during postharvest storage of both types of strawberry was divided at the fifth day of storage into two phases: postharvest changes and rotting.					
35065837	2	44	theme	quality	200:206	arg1	loss					192:195	rapid postharvest loss	174:195	rapid postharvest loss of quality and high susceptibility to microbial infections	174:254	Strawberry is very perishable fruit with rapid postharvest loss of quality and high susceptibility to microbial infections.					
35065837	3	45	from	composition	329:339	arg1	strawberry					344:353	strawberry	344:353	strawberry	344:353	In this work we study pectin modifications and microbiota and mycobiota composition in strawberry in conventional and organic cultivation systems.					
35065837	3	45	from	composition	329:339	arg1	systems					395:401	conventional and organic cultivation systems	358:401	conventional and organic cultivation systems	358:401	In this work we study pectin modifications and microbiota and mycobiota composition in strawberry in conventional and organic cultivation systems.					
35065837	3	46	from	modifications	286:298	arg1	strawberry					344:353	strawberry	344:353	strawberry	344:353	In this work we study pectin modifications and microbiota and mycobiota composition in strawberry in conventional and organic cultivation systems.					
35065837	3	46	from	modifications	286:298	arg1	systems					395:401	conventional and organic cultivation systems	358:401	conventional and organic cultivation systems	358:401	In this work we study pectin modifications and microbiota and mycobiota composition in strawberry in conventional and organic cultivation systems.					
35065837	5	47	theme	Pectin	572:577	arg1	molecules					579:587	Pectin molecules	572:587	Pectin molecules extracted from organic strawberries	572:623	Pectin molecules extracted from organic strawberries were longer and more branched compared to the conventional strawberries; however a more noticeable reorganization of molecular structure occurred.					
35065837	4	48	theme	types	462:466	arg1	storage					446:452	postharvest storage	434:452	postharvest storage of both types of strawberry	434:480	The enzymatic activity during postharvest storage of both types of strawberry was divided at the fifth day of storage into two phases: postharvest changes and rotting.					
35065837	6	49	theme	sequential	776:785	arg1	action					787:792	The sequential action	772:792	The sequential action of the pectinolytic enzymes	772:820	The sequential action of the pectinolytic enzymes had a direct effect on the molecular structure of pectin fractions.					
35065837	0	50	theme	strawberry	67:76	arg1	fruit					78:82	strawberry fruit	67:82	strawberry fruit	67:82	Changes of pectin structure and microbial community composition in strawberry fruit (Fragaria × ananassa Duch.)					
35065837	3	51	from	microbiota	304:313	arg1	strawberry					344:353	strawberry	344:353	strawberry	344:353	In this work we study pectin modifications and microbiota and mycobiota composition in strawberry in conventional and organic cultivation systems.					
35065837	3	51	from	microbiota	304:313	arg1	systems					395:401	conventional and organic cultivation systems	358:401	conventional and organic cultivation systems	358:401	In this work we study pectin modifications and microbiota and mycobiota composition in strawberry in conventional and organic cultivation systems.					
35065837	4	52	theme	storage	514:520	arg1	day					507:509	the fifth day	497:509	the fifth day of storage	497:520	The enzymatic activity during postharvest storage of both types of strawberry was divided at the fifth day of storage into two phases: postharvest changes and rotting.					
35065837	7	53	from	changes	903:909	arg1	structure					921:929	pectin structure	914:929	pectin structure	914:929	The observed changes in pectin structure relate to the synergistic activity of pectinolytic enzymes and some microorganisms.					
35065837	6	54	contain	had	822:824	arg2	effect					835:840	a direct effect	826:840	a direct effect	826:840	The sequential action of the pectinolytic enzymes had a direct effect on the molecular structure of pectin fractions.					
35065837	6	54	contain	had	822:824	arg1	action					787:792	The sequential action	772:792	The sequential action of the pectinolytic enzymes	772:820	The sequential action of the pectinolytic enzymes had a direct effect on the molecular structure of pectin fractions.					
35866893	5	0	theme	hatched	723:729	arg1	chicks					731:736	newly hatched chicks	717:736	newly hatched chicks	717:736	Firstly, newly hatched chicks were transported for 0, 2, 4, and 8 h, respectively.					
35866893	8	1	theme	molecules	1220:1228	arg1	expression					1202:1211	augmented mRNA expression	1187:1211	augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis	1187:1266	Meanwhile, transport stress also induced disturbances in glucose metabolism, reflected by augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis.					
35866893	4	2	theme	stress-induced	650:663	arg1	disorder					665:672	transport stress-induced disorder	640:672	transport stress-induced disorder of hepatic glucolipid metabolism	640:705	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35866893	10	3	theme	stress-induced	1677:1690	arg1	alterations					1692:1702	transport stress-induced alterations	1667:1702	transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules	1667:1805	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	2	4	theme	psychological	302:314	arg1	stressors					316:324	multiple physical and psychological stressors	280:324	multiple physical and psychological stressors	280:324	Stress caused by multiple physical and psychological stressors during transportation is particularly harmful to the liver.					
35866893	4	5	from	metabolism	544:553	arg1	regulation					588:597	metabolic regulation	578:597	metabolic regulation	578:597	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35866893	2	6	theme	physical	289:296	arg1	stressors					316:324	multiple physical and psychological stressors	280:324	multiple physical and psychological stressors	280:324	Stress caused by multiple physical and psychological stressors during transportation is particularly harmful to the liver.					
35866893	10	7	theme	synthesis	1712:1720	arg1	alterations					1692:1702	transport stress-induced alterations	1667:1702	transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules	1667:1805	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	11	8	theme	signaling	1977:1985	arg1	system					1987:1992	PGC-1α/SIRT1/AMPK/PPARα/PPARγ signaling system	1947:1992	PGC-1α/SIRT1/AMPK/PPARα/PPARγ signaling system	1947:1992	These findings indicated that APS could prevent the potential against transport stress-induced hepatic glucolipid metabolism disorders via PGC-1α/SIRT1/AMPK/PPARα/PPARγ signaling system.					
35866893	10	9	theme	APS	1491:1493	arg1	treatment					1495:1503	APS treatment	1491:1503	APS treatment	1491:1503	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	3	10	contain	possesses	418:426	arg1	APS					413:415	APS	413:415	APS	413:415	Astragalus polysaccharide (APS) possesses multiple benefits against hepatic metabolic disorders.					
35866893	3	10	contain	possesses	418:426	arg1	polysaccharide					397:410	Astragalus polysaccharide	386:410	Astragalus polysaccharide (APS)	386:416	Astragalus polysaccharide (APS) possesses multiple benefits against hepatic metabolic disorders.					
35866893	3	10	contain	possesses	418:426	arg2	benefits					437:444	multiple benefits	428:444	multiple benefits	428:444	Astragalus polysaccharide (APS) possesses multiple benefits against hepatic metabolic disorders.					
35866893	4	11	theme	transport	494:502	arg1	stress					504:509	transport stress	494:509	transport stress	494:509	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35866893	7	12	theme	lipid	1070:1074	arg1	deposition					1076:1085	transport stress-induced lipid deposition	1045:1085	transport stress-induced lipid deposition in liver	1045:1094	Our study suggested that APS could relieve transport stress-induced lipid deposition in liver.					
35866893	6	13	theme	transport	981:989	arg1	treatment					991:999	transport treatment	981:999	transport treatment	981:999	Subsequently, to further investigate the effects of APS on transport stress-induced hepatic glucolipid metabolism disturbance, chicks were pretreated with water or APS and then subjected to transport treatment.					
35866893	10	14	theme	cholesterol	1723:1733	arg1	metabolism					1735:1744	cholesterol metabolism	1723:1744	cholesterol metabolism	1723:1744	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	9	15	theme	peroxisome	1340:1349	arg1	alpha					1403:1407	peroxisome proliferator-activated receptor gamma coactivator 1 alpha	1340:1407	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway	1340:1478	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	9	15	theme	peroxisome	1340:1349	arg1	PGC-1α					1410:1415	PGC-1α	1410:1415	PGC-1α	1410:1415	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	7	16	theme	transport	1045:1053	arg1	deposition					1076:1085	transport stress-induced lipid deposition	1045:1085	transport stress-induced lipid deposition in liver	1045:1094	Our study suggested that APS could relieve transport stress-induced lipid deposition in liver.					
35866893	11	17	theme	transport	1878:1886	arg1	metabolism					1922:1931	transport stress-induced hepatic glucolipid metabolism	1878:1931	transport stress-induced hepatic glucolipid metabolism disorders	1878:1941	These findings indicated that APS could prevent the potential against transport stress-induced hepatic glucolipid metabolism disorders via PGC-1α/SIRT1/AMPK/PPARα/PPARγ signaling system.					
35866893	9	18	theme	receptor	1374:1381	arg1	alpha					1403:1407	peroxisome proliferator-activated receptor gamma coactivator 1 alpha	1340:1407	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway	1340:1478	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	9	18	theme	receptor	1374:1381	arg1	PGC-1α					1410:1415	PGC-1α	1410:1415	PGC-1α	1410:1415	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	1	19	theme	poultry	155:161	arg1	industry					163:170	the modern poultry industry	144:170	the modern poultry industry	144:170	In the modern poultry industry, newly hatched chicks are unavoidably transported from the hatching to the rearing foster.					
35866893	9	20	theme	coactivator	1389:1399	arg1	alpha					1403:1407	peroxisome proliferator-activated receptor gamma coactivator 1 alpha	1340:1407	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway	1340:1478	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	9	20	theme	coactivator	1389:1399	arg1	PGC-1α					1410:1415	PGC-1α	1410:1415	PGC-1α	1410:1415	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	10	21	theme	peroxisome	1528:1537	arg1	PPARα					1578:1582	PPARα	1578:1582	PPARα	1578:1582	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	10	21	theme	peroxisome	1528:1537	arg1	alpha					1571:1575	peroxisome proliferator-activated receptor alpha	1528:1575	peroxisome proliferator-activated receptor alpha (PPARα)	1528:1583	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	11	22	theme	metabolism	1922:1931	arg1	disorders					1933:1941	transport stress-induced hepatic glucolipid metabolism disorders	1878:1941	transport stress-induced hepatic glucolipid metabolism disorders	1878:1941	These findings indicated that APS could prevent the potential against transport stress-induced hepatic glucolipid metabolism disorders via PGC-1α/SIRT1/AMPK/PPARα/PPARγ signaling system.					
35866893	0	23	theme	hepatic	100:106	arg1	metabolism					119:128	hepatic glucolipid metabolism	100:128	hepatic glucolipid metabolism	100:128	Astragalus polysaccharide mitigates transport stress-induced hepatic metabolic stress via improving hepatic glucolipid metabolism in chicks.					
35866893	9	24	theme	alpha	1403:1407	arg1	kinase					1458:1463	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase	1340:1463	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway	1340:1478	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	9	24	theme	alpha	1403:1407	arg1	AMPK					1466:1469	AMPK	1466:1469	AMPK	1466:1469	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	1	25	theme	hatched	179:185	arg1	chicks					187:192	newly hatched chicks	173:192	newly hatched chicks	173:192	In the modern poultry industry, newly hatched chicks are unavoidably transported from the hatching to the rearing foster.					
35866893	11	26	theme	hepatic	1903:1909	arg1	metabolism					1922:1931	transport stress-induced hepatic glucolipid metabolism	1878:1931	transport stress-induced hepatic glucolipid metabolism disorders	1878:1941	These findings indicated that APS could prevent the potential against transport stress-induced hepatic glucolipid metabolism disorders via PGC-1α/SIRT1/AMPK/PPARα/PPARγ signaling system.					
35866893	4	27	theme	hepatic	525:531	arg1	metabolism					544:553	hepatic glucolipid metabolism	525:553	hepatic glucolipid metabolism	525:553	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35866893	9	28	theme	/Sirtuin	1417:1424	arg1	kinase					1458:1463	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase	1340:1463	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway	1340:1478	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	9	28	theme	/Sirtuin	1417:1424	arg1	AMPK					1466:1469	AMPK	1466:1469	AMPK	1466:1469	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	6	29	from	effects	832:838	arg1	disturbance					905:915	transport stress-induced hepatic glucolipid metabolism disturbance	850:915	transport stress-induced hepatic glucolipid metabolism disturbance	850:915	Subsequently, to further investigate the effects of APS on transport stress-induced hepatic glucolipid metabolism disturbance, chicks were pretreated with water or APS and then subjected to transport treatment.					
35866893	10	30	theme	receptor	1562:1569	arg1	PPARα					1578:1582	PPARα	1578:1582	PPARα	1578:1582	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	10	30	theme	receptor	1562:1569	arg1	alpha					1571:1575	peroxisome proliferator-activated receptor alpha	1528:1575	peroxisome proliferator-activated receptor alpha (PPARα)	1528:1583	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	0	31	theme	Astragalus	0:9	arg1	polysaccharide					11:24	Astragalus polysaccharide	0:24	Astragalus polysaccharide	0:24	Astragalus polysaccharide mitigates transport stress-induced hepatic metabolic stress via improving hepatic glucolipid metabolism in chicks.					
35866893	10	32	theme	peroxisome	1589:1598	arg1	PPARγ					1639:1643	PPARγ	1639:1643	PPARγ	1639:1643	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	10	32	theme	peroxisome	1589:1598	arg1	gamma					1632:1636	peroxisome proliferator-activated receptor gamma	1589:1636	peroxisome proliferator-activated receptor gamma (PPARγ)	1589:1644	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	3	33	theme	hepatic	454:460	arg1	disorders					472:480	hepatic metabolic disorders	454:480	hepatic metabolic disorders	454:480	Astragalus polysaccharide (APS) possesses multiple benefits against hepatic metabolic disorders.					
35866893	10	34	theme	transport-related	1779:1795	arg1	molecules					1797:1805	transport-related molecules	1779:1805	transport-related molecules	1779:1805	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	0	35	theme	stress-induced	46:59	arg1	stress					79:84	transport stress-induced hepatic metabolic stress	36:84	transport stress-induced hepatic metabolic stress	36:84	Astragalus polysaccharide mitigates transport stress-induced hepatic metabolic stress via improving hepatic glucolipid metabolism in chicks.					
35866893	9	36	theme	protein	1450:1456	arg1	kinase					1458:1463	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase	1340:1463	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway	1340:1478	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	9	36	theme	protein	1450:1456	arg1	AMPK					1466:1469	AMPK	1466:1469	AMPK	1466:1469	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	0	37	theme	metabolic	69:77	arg1	stress					79:84	transport stress-induced hepatic metabolic stress	36:84	transport stress-induced hepatic metabolic stress	36:84	Astragalus polysaccharide mitigates transport stress-induced hepatic metabolic stress via improving hepatic glucolipid metabolism in chicks.					
35866893	6	38	theme	glucolipid	883:892	arg1	disturbance					905:915	transport stress-induced hepatic glucolipid metabolism disturbance	850:915	transport stress-induced hepatic glucolipid metabolism disturbance	850:915	Subsequently, to further investigate the effects of APS on transport stress-induced hepatic glucolipid metabolism disturbance, chicks were pretreated with water or APS and then subjected to transport treatment.					
35866893	10	39	theme	receptor	1623:1630	arg1	PPARγ					1639:1643	PPARγ	1639:1643	PPARγ	1639:1643	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	10	39	theme	receptor	1623:1630	arg1	gamma					1632:1636	peroxisome proliferator-activated receptor gamma	1589:1636	peroxisome proliferator-activated receptor gamma (PPARγ)	1589:1644	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	6	40	theme	stress-induced	860:873	arg1	disturbance					905:915	transport stress-induced hepatic glucolipid metabolism disturbance	850:915	transport stress-induced hepatic glucolipid metabolism disturbance	850:915	Subsequently, to further investigate the effects of APS on transport stress-induced hepatic glucolipid metabolism disturbance, chicks were pretreated with water or APS and then subjected to transport treatment.					
35866893	8	41	theme	augmented	1187:1195	arg1	expression					1202:1211	augmented mRNA expression	1187:1211	augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis	1187:1266	Meanwhile, transport stress also induced disturbances in glucose metabolism, reflected by augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis.					
35866893	4	42	theme	hepatic	677:683	arg1	metabolism					696:705	hepatic glucolipid metabolism	677:705	hepatic glucolipid metabolism	677:705	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35866893	4	43	from	role	563:566	arg1	regulation					588:597	metabolic regulation	578:597	metabolic regulation	578:597	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35866893	6	44	theme	APS	843:845	arg1	effects					832:838	the effects	828:838	the effects of APS on transport stress-induced hepatic glucolipid metabolism disturbance	828:915	Subsequently, to further investigate the effects of APS on transport stress-induced hepatic glucolipid metabolism disturbance, chicks were pretreated with water or APS and then subjected to transport treatment.					
35866893	8	45	theme	key	1216:1218	arg1	molecules					1220:1228	key molecules	1216:1228	key molecules	1216:1228	Meanwhile, transport stress also induced disturbances in glucose metabolism, reflected by augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis.					
35866893	4	46	theme	transport	640:648	arg1	disorder					665:672	transport stress-induced disorder	640:672	transport stress-induced disorder of hepatic glucolipid metabolism	640:705	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35866893	10	47	theme	transport	1667:1675	arg1	alterations					1692:1702	transport stress-induced alterations	1667:1702	transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules	1667:1805	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	10	48	theme	VLDL	1707:1710	arg1	synthesis					1712:1720	VLDL synthesis	1707:1720	VLDL synthesis	1707:1720	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	4	49	theme	metabolism	696:705	arg1	disorder					665:672	transport stress-induced disorder	640:672	transport stress-induced disorder of hepatic glucolipid metabolism	640:705	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35866893	10	50	theme	lipid	1747:1751	arg1	oxidation					1753:1761	lipid oxidation	1747:1761	lipid oxidation	1747:1761	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	11	51	theme	PGC-1α/SIRT1/AMPK/PPARα/PPARγ	1947:1975	arg1	system					1987:1992	PGC-1α/SIRT1/AMPK/PPARα/PPARγ signaling system	1947:1992	PGC-1α/SIRT1/AMPK/PPARα/PPARγ signaling system	1947:1992	These findings indicated that APS could prevent the potential against transport stress-induced hepatic glucolipid metabolism disorders via PGC-1α/SIRT1/AMPK/PPARα/PPARγ signaling system.					
35866893	7	52	from	deposition	1076:1085	arg1	liver					1090:1094	liver	1090:1094	liver	1090:1094	Our study suggested that APS could relieve transport stress-induced lipid deposition in liver.					
35866893	10	53	theme	metabolism	1735:1744	arg1	alterations					1692:1702	transport stress-induced alterations	1667:1702	transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules	1667:1805	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	9	54	theme	proliferator-activated	1351:1372	arg1	alpha					1403:1407	peroxisome proliferator-activated receptor gamma coactivator 1 alpha	1340:1407	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway	1340:1478	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	9	54	theme	proliferator-activated	1351:1372	arg1	PGC-1α					1410:1415	PGC-1α	1410:1415	PGC-1α	1410:1415	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	2	55	theme	multiple	280:287	arg1	stressors					316:324	multiple physical and psychological stressors	280:324	multiple physical and psychological stressors	280:324	Stress caused by multiple physical and psychological stressors during transportation is particularly harmful to the liver.					
35866893	7	56	theme	stress-induced	1055:1068	arg1	deposition					1076:1085	transport stress-induced lipid deposition	1045:1085	transport stress-induced lipid deposition in liver	1045:1094	Our study suggested that APS could relieve transport stress-induced lipid deposition in liver.					
35866893	1	57	theme	modern	148:153	arg1	industry					163:170	the modern poultry industry	144:170	the modern poultry industry	144:170	In the modern poultry industry, newly hatched chicks are unavoidably transported from the hatching to the rearing foster.					
35866893	11	58	theme	stress-induced	1888:1901	arg1	metabolism					1922:1931	transport stress-induced hepatic glucolipid metabolism	1878:1931	transport stress-induced hepatic glucolipid metabolism disorders	1878:1941	These findings indicated that APS could prevent the potential against transport stress-induced hepatic glucolipid metabolism disorders via PGC-1α/SIRT1/AMPK/PPARα/PPARγ signaling system.					
35866893	9	59	theme	gamma	1383:1387	arg1	alpha					1403:1407	peroxisome proliferator-activated receptor gamma coactivator 1 alpha	1340:1407	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway	1340:1478	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	9	59	theme	gamma	1383:1387	arg1	PGC-1α					1410:1415	PGC-1α	1410:1415	PGC-1α	1410:1415	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	8	60	from	disturbances	1138:1149	arg1	metabolism					1162:1171	glucose metabolism	1154:1171	glucose metabolism	1154:1171	Meanwhile, transport stress also induced disturbances in glucose metabolism, reflected by augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis.					
35866893	0	61	theme	glucolipid	108:117	arg1	metabolism					119:128	hepatic glucolipid metabolism	100:128	hepatic glucolipid metabolism	100:128	Astragalus polysaccharide mitigates transport stress-induced hepatic metabolic stress via improving hepatic glucolipid metabolism in chicks.					
35866893	4	62	theme	APS	571:573	arg1	metabolism					544:553	hepatic glucolipid metabolism	525:553	hepatic glucolipid metabolism	525:553	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35866893	4	62	theme	APS	571:573	arg1	role					563:566	the role	559:566	the role of APS in metabolic regulation	559:597	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35866893	4	63	theme	glucolipid	533:542	arg1	metabolism					544:553	hepatic glucolipid metabolism	525:553	hepatic glucolipid metabolism	525:553	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35866893	11	64	theme	glucolipid	1911:1920	arg1	metabolism					1922:1931	transport stress-induced hepatic glucolipid metabolism	1878:1931	transport stress-induced hepatic glucolipid metabolism disorders	1878:1941	These findings indicated that APS could prevent the potential against transport stress-induced hepatic glucolipid metabolism disorders via PGC-1α/SIRT1/AMPK/PPARα/PPARγ signaling system.					
35866893	8	65	theme	transport	1108:1116	arg1	Meanwhile					1097:1105	Meanwhile	1097:1105	Meanwhile	1097:1105	Meanwhile, transport stress also induced disturbances in glucose metabolism, reflected by augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis.					
35866893	8	65	theme	transport	1108:1116	arg1	stress					1118:1123	transport stress	1108:1123	transport stress	1108:1123	Meanwhile, transport stress also induced disturbances in glucose metabolism, reflected by augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis.					
35866893	3	66	theme	Astragalus	386:395	arg1	APS					413:415	APS	413:415	APS	413:415	Astragalus polysaccharide (APS) possesses multiple benefits against hepatic metabolic disorders.					
35866893	3	66	theme	Astragalus	386:395	arg1	polysaccharide					397:410	Astragalus polysaccharide	386:410	Astragalus polysaccharide (APS)	386:416	Astragalus polysaccharide (APS) possesses multiple benefits against hepatic metabolic disorders.					
35866893	10	67	theme	alpha	1571:1575	arg1	level					1519:1523	the level	1515:1523	the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ)	1515:1644	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	10	68	theme	synthesis	1764:1772	arg1	alterations					1692:1702	transport stress-induced alterations	1667:1702	transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules	1667:1805	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	3	69	theme	multiple	428:435	arg1	benefits					437:444	multiple benefits	428:444	multiple benefits	428:444	Astragalus polysaccharide (APS) possesses multiple benefits against hepatic metabolic disorders.					
35866893	10	70	theme	proliferator-activated	1539:1560	arg1	PPARα					1578:1582	PPARα	1578:1582	PPARα	1578:1582	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	10	70	theme	proliferator-activated	1539:1560	arg1	alpha					1571:1575	peroxisome proliferator-activated receptor alpha	1528:1575	peroxisome proliferator-activated receptor alpha (PPARα)	1528:1583	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	10	71	theme	oxidation	1753:1761	arg1	alterations					1692:1702	transport stress-induced alterations	1667:1702	transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules	1667:1805	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	9	72	theme	SIRT1	1429:1433	arg1	kinase					1458:1463	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase	1340:1463	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway	1340:1478	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	9	72	theme	SIRT1	1429:1433	arg1	AMPK					1466:1469	AMPK	1466:1469	AMPK	1466:1469	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	10	73	theme	molecules	1797:1805	arg1	alterations					1692:1702	transport stress-induced alterations	1667:1702	transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules	1667:1805	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	0	74	theme	hepatic	61:67	arg1	stress					79:84	transport stress-induced hepatic metabolic stress	36:84	transport stress-induced hepatic metabolic stress	36:84	Astragalus polysaccharide mitigates transport stress-induced hepatic metabolic stress via improving hepatic glucolipid metabolism in chicks.					
35866893	9	75	theme	/AMP-activated	1435:1448	arg1	kinase					1458:1463	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase	1340:1463	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway	1340:1478	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	9	75	theme	/AMP-activated	1435:1448	arg1	AMPK					1466:1469	AMPK	1466:1469	AMPK	1466:1469	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	8	76	theme	glucose	1154:1160	arg1	metabolism					1162:1171	glucose metabolism	1154:1171	glucose metabolism	1154:1171	Meanwhile, transport stress also induced disturbances in glucose metabolism, reflected by augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis.					
35866893	8	77	from	expression	1202:1211	arg1	glycogenolysis					1253:1266	glycogenolysis	1253:1266	glycogenolysis	1253:1266	Meanwhile, transport stress also induced disturbances in glucose metabolism, reflected by augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis.					
35866893	8	77	from	expression	1202:1211	arg1	gluconeogenesis					1233:1247	gluconeogenesis	1233:1247	gluconeogenesis	1233:1247	Meanwhile, transport stress also induced disturbances in glucose metabolism, reflected by augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis.					
35866893	3	78	theme	metabolic	462:470	arg1	disorders					472:480	hepatic metabolic disorders	454:480	hepatic metabolic disorders	454:480	Astragalus polysaccharide (APS) possesses multiple benefits against hepatic metabolic disorders.					
35866893	4	79	theme	metabolic	578:586	arg1	regulation					588:597	metabolic regulation	578:597	metabolic regulation	578:597	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35866893	9	80	theme	kinase	1458:1463	arg1	pathway					1472:1478	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway	1340:1478	peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway	1340:1478	Surprisingly, APS could simultaneously alleviate these alterations via peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α)/Sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway.					
35866893	6	81	theme	metabolism	894:903	arg1	disturbance					905:915	transport stress-induced hepatic glucolipid metabolism disturbance	850:915	transport stress-induced hepatic glucolipid metabolism disturbance	850:915	Subsequently, to further investigate the effects of APS on transport stress-induced hepatic glucolipid metabolism disturbance, chicks were pretreated with water or APS and then subjected to transport treatment.					
35866893	10	82	theme	gamma	1632:1636	arg1	level					1519:1523	the level	1515:1523	the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ)	1515:1644	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	6	83	theme	hepatic	875:881	arg1	disturbance					905:915	transport stress-induced hepatic glucolipid metabolism disturbance	850:915	transport stress-induced hepatic glucolipid metabolism disturbance	850:915	Subsequently, to further investigate the effects of APS on transport stress-induced hepatic glucolipid metabolism disturbance, chicks were pretreated with water or APS and then subjected to transport treatment.					
35866893	10	84	theme	proliferator-activated	1600:1621	arg1	PPARγ					1639:1643	PPARγ	1639:1643	PPARγ	1639:1643	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	10	84	theme	proliferator-activated	1600:1621	arg1	gamma					1632:1636	peroxisome proliferator-activated receptor gamma	1589:1636	peroxisome proliferator-activated receptor gamma (PPARγ)	1589:1644	Moreover, APS treatment regulated the level of peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ), thereby alleviating transport stress-induced alterations of VLDL synthesis, cholesterol metabolism, lipid oxidation, synthesis, and transport-related molecules.					
35866893	1	85	theme	rearing	247:253	arg1	foster					255:260	the rearing foster	243:260	the rearing foster	243:260	In the modern poultry industry, newly hatched chicks are unavoidably transported from the hatching to the rearing foster.					
35866893	8	86	theme	mRNA	1197:1200	arg1	expression					1202:1211	augmented mRNA expression	1187:1211	augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis	1187:1266	Meanwhile, transport stress also induced disturbances in glucose metabolism, reflected by augmented mRNA expression of key molecules in gluconeogenesis and glycogenolysis.					
35866893	6	87	theme	transport	850:858	arg1	disturbance					905:915	transport stress-induced hepatic glucolipid metabolism disturbance	850:915	transport stress-induced hepatic glucolipid metabolism disturbance	850:915	Subsequently, to further investigate the effects of APS on transport stress-induced hepatic glucolipid metabolism disturbance, chicks were pretreated with water or APS and then subjected to transport treatment.					
35866893	4	88	theme	glucolipid	685:694	arg1	metabolism					696:705	hepatic glucolipid metabolism	677:705	hepatic glucolipid metabolism	677:705	Given that transport stress could disturb hepatic glucolipid metabolism and the role of APS in metabolic regulation, we speculated that APS could antagonize transport stress-induced disorder of hepatic glucolipid metabolism.					
35422282	0	0	theme	oil	103:105	arg1	cleanup					113:119	oil slick cleanup	103:119	oil slick cleanup	103:119	Superhydrophobic starch-based nanocomposite cryogel for oil removal underwater and magnetically guided oil slick cleanup.					
35422282	5	1	theme	chemical	798:805	arg1	composition					807:817	surface chemical composition	790:817	surface chemical composition	790:817	In addition, the surface chemical composition and microstructure of the adsorbent were investigated in detail.					
35422282	1	2	theme	great	193:197	arg1	attention					199:207	great attention	193:207	great attention	193:207	Low-cost and eco-friendly carbohydrate-based absorbents have attracted great attention for cleaning oil spill that poses a serious threat to the ecosystem.					
35422282	7	3	theme	cleaning	1140:1147	arg1	slick					1153:1157	magnetically guided cleaning oil slick	1120:1157	magnetically guided cleaning oil slick	1120:1157	Furthermore, HMS-SiO2@MSC exhibited good practicability of removing oil underwater and magnetically guided cleaning oil slick on the water surface.					
35422282	4	4	theme	SiO2	609:612	arg1	nanoparticles					614:626	Fe3O4 and silylated SiO2 nanoparticles	589:626	Fe3O4 and silylated SiO2 nanoparticles	589:626	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	3	5	theme	superhydrophobic	484:499	arg1	MSC					521:523	HMS-SiO2@MSC	512:523	HMS-SiO2@MSC	512:523	Herein, a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC) was achieved by introducing nanoparticles into starch cryogel.					
35422282	3	5	theme	superhydrophobic	484:499	arg1	absorbent					501:509	a novel starch-based superhydrophobic absorbent	463:509	a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC)	463:524	Herein, a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC) was achieved by introducing nanoparticles into starch cryogel.					
35422282	4	6	with	endowed	628:634	arg1	magnetism					655:663	magnetism	655:663	magnetism (saturation magnetization: 4.36 emu/g)	655:702	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	4	6	with	endowed	628:634	arg1	4.36 emu/g					692:701	saturation magnetization: 4.36 emu/g	666:701	saturation magnetization: 4.36 emu/g	666:701	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	4	6	with	endowed	628:634	arg1	superhydrophobicity					708:726	superhydrophobicity	708:726	superhydrophobicity (water contact angle: 154.4°)	708:756	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	7	7	theme	water	1166:1170	arg1	surface					1172:1178	the water surface	1162:1178	the water surface	1162:1178	Furthermore, HMS-SiO2@MSC exhibited good practicability of removing oil underwater and magnetically guided cleaning oil slick on the water surface.					
35422282	3	8	theme	starch	573:578	arg1	cryogel					580:586	starch cryogel	573:586	starch cryogel	573:586	Herein, a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC) was achieved by introducing nanoparticles into starch cryogel.					
35422282	0	9	theme	slick	107:111	arg1	cleanup					113:119	oil slick cleanup	103:119	oil slick cleanup	103:119	Superhydrophobic starch-based nanocomposite cryogel for oil removal underwater and magnetically guided oil slick cleanup.					
35422282	4	10	dep	angle	743:747	arg1	154.4°					750:755	154.4°	750:755	water contact angle: 154.4°	729:755	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	5	11	theme	adsorbent	845:853	arg1	microstructure					823:836	microstructure	823:836	microstructure	823:836	In addition, the surface chemical composition and microstructure of the adsorbent were investigated in detail.					
35422282	5	11	theme	adsorbent	845:853	arg1	composition					807:817	surface chemical composition	790:817	surface chemical composition	790:817	In addition, the surface chemical composition and microstructure of the adsorbent were investigated in detail.					
35422282	3	12	theme	novel	465:469	arg1	MSC					521:523	HMS-SiO2@MSC	512:523	HMS-SiO2@MSC	512:523	Herein, a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC) was achieved by introducing nanoparticles into starch cryogel.					
35422282	3	12	theme	novel	465:469	arg1	absorbent					501:509	a novel starch-based superhydrophobic absorbent	463:509	a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC)	463:524	Herein, a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC) was achieved by introducing nanoparticles into starch cryogel.					
35422282	0	13	theme	starch-based	17:28	arg1	cryogel					44:50	Superhydrophobic starch-based nanocomposite cryogel	0:50	Superhydrophobic starch-based nanocomposite cryogel for oil removal	0:66	Superhydrophobic starch-based nanocomposite cryogel for oil removal underwater and magnetically guided oil slick cleanup.					
35422282	6	14	theme	MSC	920:922	arg1	properties					897:906	Some crucial properties	884:906	Some crucial properties of HMS-SiO2@MSC	884:922	Some crucial properties of HMS-SiO2@MSC were also comprehensively confirmed, including water-repellent, self-cleaning, anti-fouling, and durability.					
35422282	7	15	theme	oil	1149:1151	arg1	slick					1153:1157	magnetically guided cleaning oil slick	1120:1157	magnetically guided cleaning oil slick	1120:1157	Furthermore, HMS-SiO2@MSC exhibited good practicability of removing oil underwater and magnetically guided cleaning oil slick on the water surface.					
35422282	2	16	theme	absorption	342:351	arg1	performance					353:363	good oil absorption performance	333:363	good oil absorption performance	333:363	Porous starch-based materials have been proven to have good oil absorption performance, but the hydrophilicity of these materials limits their application in oil spill cleanup.					
35422282	1	17	theme	oil	222:224	arg1	spill					226:230	oil spill	222:230	oil spill that poses a serious threat to the ecosystem	222:275	Low-cost and eco-friendly carbohydrate-based absorbents have attracted great attention for cleaning oil spill that poses a serious threat to the ecosystem.					
35422282	0	18	theme	Superhydrophobic	0:15	arg1	cryogel					44:50	Superhydrophobic starch-based nanocomposite cryogel	0:50	Superhydrophobic starch-based nanocomposite cryogel for oil removal	0:66	Superhydrophobic starch-based nanocomposite cryogel for oil removal underwater and magnetically guided oil slick cleanup.					
35422282	4	19	theme	Fe3O4	589:593	arg1	nanoparticles					614:626	Fe3O4 and silylated SiO2 nanoparticles	589:626	Fe3O4 and silylated SiO2 nanoparticles	589:626	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	2	20	theme	oil	338:340	arg1	performance					353:363	good oil absorption performance	333:363	good oil absorption performance	333:363	Porous starch-based materials have been proven to have good oil absorption performance, but the hydrophilicity of these materials limits their application in oil spill cleanup.					
35422282	6	21	theme	HMS-SiO2	911:918	arg1	MSC					920:922	HMS-SiO2@MSC	911:922	HMS-SiO2@MSC	911:922	Some crucial properties of HMS-SiO2@MSC were also comprehensively confirmed, including water-repellent, self-cleaning, anti-fouling, and durability.					
35422282	8	22	theme	application	1260:1270	arg1	fields					1272:1277	the application fields	1256:1277	the application fields of starch	1256:1287	This work provided a new carbohydrate-based adsorbent, which would broaden the application fields of starch.					
35422282	7	23	theme	guided	1133:1138	arg1	slick					1153:1157	magnetically guided cleaning oil slick	1120:1157	magnetically guided cleaning oil slick	1120:1157	Furthermore, HMS-SiO2@MSC exhibited good practicability of removing oil underwater and magnetically guided cleaning oil slick on the water surface.					
35422282	2	24	theme	good	333:336	arg1	performance					353:363	good oil absorption performance	333:363	good oil absorption performance	333:363	Porous starch-based materials have been proven to have good oil absorption performance, but the hydrophilicity of these materials limits their application in oil spill cleanup.					
35422282	4	25	theme	magnetization	677:689	arg1	4.36 emu/g					692:701	saturation magnetization: 4.36 emu/g	666:701	saturation magnetization: 4.36 emu/g	666:701	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	4	25	theme	magnetization	677:689	arg1	magnetism					655:663	magnetism	655:663	magnetism (saturation magnetization: 4.36 emu/g)	655:702	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	0	26	theme	nanocomposite	30:42	arg1	cryogel					44:50	Superhydrophobic starch-based nanocomposite cryogel	0:50	Superhydrophobic starch-based nanocomposite cryogel for oil removal	0:66	Superhydrophobic starch-based nanocomposite cryogel for oil removal underwater and magnetically guided oil slick cleanup.					
35422282	4	27	theme	silylated	599:607	arg1	nanoparticles					614:626	Fe3O4 and silylated SiO2 nanoparticles	589:626	Fe3O4 and silylated SiO2 nanoparticles	589:626	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	5	28	dep	composition	807:817	arg1	the					786:788	the	786:788	the	786:788	In addition, the surface chemical composition and microstructure of the adsorbent were investigated in detail.					
35422282	6	29	theme	@	919:919	arg1	MSC					920:922	HMS-SiO2@MSC	911:922	HMS-SiO2@MSC	911:922	Some crucial properties of HMS-SiO2@MSC were also comprehensively confirmed, including water-repellent, self-cleaning, anti-fouling, and durability.					
35422282	1	30	theme	Low-cost	122:129	arg1	absorbents					167:176	Low-cost and eco-friendly carbohydrate-based absorbents	122:176	Low-cost and eco-friendly carbohydrate-based absorbents	122:176	Low-cost and eco-friendly carbohydrate-based absorbents have attracted great attention for cleaning oil spill that poses a serious threat to the ecosystem.					
35422282	4	31	theme	saturation	666:675	arg1	4.36 emu/g					692:701	saturation magnetization: 4.36 emu/g	666:701	saturation magnetization: 4.36 emu/g	666:701	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	4	31	theme	saturation	666:675	arg1	magnetism					655:663	magnetism	655:663	magnetism (saturation magnetization: 4.36 emu/g)	655:702	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	0	32	theme	oil	56:58	arg1	removal					60:66	oil removal	56:66	oil removal	56:66	Superhydrophobic starch-based nanocomposite cryogel for oil removal underwater and magnetically guided oil slick cleanup.					
35422282	3	33	theme	starch-based	471:482	arg1	MSC					521:523	HMS-SiO2@MSC	512:523	HMS-SiO2@MSC	512:523	Herein, a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC) was achieved by introducing nanoparticles into starch cryogel.					
35422282	3	33	theme	starch-based	471:482	arg1	absorbent					501:509	a novel starch-based superhydrophobic absorbent	463:509	a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC)	463:524	Herein, a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC) was achieved by introducing nanoparticles into starch cryogel.					
35422282	2	34	theme	spill	440:444	arg1	cleanup					446:452	oil spill cleanup	436:452	oil spill cleanup	436:452	Porous starch-based materials have been proven to have good oil absorption performance, but the hydrophilicity of these materials limits their application in oil spill cleanup.					
35422282	4	35	theme	contact	735:741	arg1	angle					743:747	water contact angle	729:747	water contact angle: 154.4°	729:755	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	2	36	contain	have	328:331	arg2	performance					353:363	good oil absorption performance	333:363	good oil absorption performance	333:363	Porous starch-based materials have been proven to have good oil absorption performance, but the hydrophilicity of these materials limits their application in oil spill cleanup.					
35422282	2	36	contain	have	328:331	arg1	materials					298:306	Porous starch-based materials	278:306	Porous starch-based materials	278:306	Porous starch-based materials have been proven to have good oil absorption performance, but the hydrophilicity of these materials limits their application in oil spill cleanup.					
35422282	7	37	theme	good	1069:1072	arg1	practicability					1074:1087	good practicability	1069:1087	good practicability of removing oil underwater and magnetically guided cleaning oil slick on the water surface	1069:1178	Furthermore, HMS-SiO2@MSC exhibited good practicability of removing oil underwater and magnetically guided cleaning oil slick on the water surface.					
35422282	1	38	theme	serious	245:251	arg1	threat					253:258	a serious threat	243:258	a serious threat to the ecosystem	243:275	Low-cost and eco-friendly carbohydrate-based absorbents have attracted great attention for cleaning oil spill that poses a serious threat to the ecosystem.					
35422282	7	39	theme	oil	1101:1103	arg1	underwater					1105:1114	oil underwater	1101:1114	oil underwater	1101:1114	Furthermore, HMS-SiO2@MSC exhibited good practicability of removing oil underwater and magnetically guided cleaning oil slick on the water surface.					
35422282	8	40	theme	starch	1282:1287	arg1	fields					1272:1277	the application fields	1256:1277	the application fields of starch	1256:1287	This work provided a new carbohydrate-based adsorbent, which would broaden the application fields of starch.					
35422282	6	41	theme	crucial	889:895	arg1	properties					897:906	Some crucial properties	884:906	Some crucial properties of HMS-SiO2@MSC	884:922	Some crucial properties of HMS-SiO2@MSC were also comprehensively confirmed, including water-repellent, self-cleaning, anti-fouling, and durability.					
35422282	1	42	theme	eco-friendly	135:146	arg1	absorbents					167:176	Low-cost and eco-friendly carbohydrate-based absorbents	122:176	Low-cost and eco-friendly carbohydrate-based absorbents	122:176	Low-cost and eco-friendly carbohydrate-based absorbents have attracted great attention for cleaning oil spill that poses a serious threat to the ecosystem.					
35422282	2	43	from	application	421:431	arg1	cleanup					446:452	oil spill cleanup	436:452	oil spill cleanup	436:452	Porous starch-based materials have been proven to have good oil absorption performance, but the hydrophilicity of these materials limits their application in oil spill cleanup.					
35422282	1	44	theme	carbohydrate-based	148:165	arg1	absorbents					167:176	Low-cost and eco-friendly carbohydrate-based absorbents	122:176	Low-cost and eco-friendly carbohydrate-based absorbents	122:176	Low-cost and eco-friendly carbohydrate-based absorbents have attracted great attention for cleaning oil spill that poses a serious threat to the ecosystem.					
35422282	2	45	theme	starch-based	285:296	arg1	materials					298:306	Porous starch-based materials	278:306	Porous starch-based materials	278:306	Porous starch-based materials have been proven to have good oil absorption performance, but the hydrophilicity of these materials limits their application in oil spill cleanup.					
35422282	7	46	theme	HMS-SiO2	1046:1053	arg1	MSC					1055:1057	HMS-SiO2@MSC	1046:1057	HMS-SiO2@MSC	1046:1057	Furthermore, HMS-SiO2@MSC exhibited good practicability of removing oil underwater and magnetically guided cleaning oil slick on the water surface.					
35422282	3	47	theme	@	520:520	arg1	MSC					521:523	HMS-SiO2@MSC	512:523	HMS-SiO2@MSC	512:523	Herein, a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC) was achieved by introducing nanoparticles into starch cryogel.					
35422282	3	47	theme	@	520:520	arg1	absorbent					501:509	a novel starch-based superhydrophobic absorbent	463:509	a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC)	463:524	Herein, a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC) was achieved by introducing nanoparticles into starch cryogel.					
35422282	3	48	theme	HMS-SiO2	512:519	arg1	MSC					521:523	HMS-SiO2@MSC	512:523	HMS-SiO2@MSC	512:523	Herein, a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC) was achieved by introducing nanoparticles into starch cryogel.					
35422282	3	48	theme	HMS-SiO2	512:519	arg1	absorbent					501:509	a novel starch-based superhydrophobic absorbent	463:509	a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC)	463:524	Herein, a novel starch-based superhydrophobic absorbent (HMS-SiO2@MSC) was achieved by introducing nanoparticles into starch cryogel.					
35422282	2	49	theme	oil	436:438	arg1	cleanup					446:452	oil spill cleanup	436:452	oil spill cleanup	436:452	Porous starch-based materials have been proven to have good oil absorption performance, but the hydrophilicity of these materials limits their application in oil spill cleanup.					
35422282	2	50	theme	Porous	278:283	arg1	materials					298:306	Porous starch-based materials	278:306	Porous starch-based materials	278:306	Porous starch-based materials have been proven to have good oil absorption performance, but the hydrophilicity of these materials limits their application in oil spill cleanup.					
35422282	7	51	theme	@	1054:1054	arg1	MSC					1055:1057	HMS-SiO2@MSC	1046:1057	HMS-SiO2@MSC	1046:1057	Furthermore, HMS-SiO2@MSC exhibited good practicability of removing oil underwater and magnetically guided cleaning oil slick on the water surface.					
35422282	2	52	theme	materials	398:406	arg1	hydrophilicity					374:387	the hydrophilicity	370:387	the hydrophilicity of these materials	370:406	Porous starch-based materials have been proven to have good oil absorption performance, but the hydrophilicity of these materials limits their application in oil spill cleanup.					
35422282	5	53	theme	surface	790:796	arg1	composition					807:817	surface chemical composition	790:817	surface chemical composition	790:817	In addition, the surface chemical composition and microstructure of the adsorbent were investigated in detail.					
35422282	4	54	dep	superhydrophobicity	708:726	arg1	angle					743:747	water contact angle	729:747	water contact angle: 154.4°	729:755	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
35422282	4	55	theme	water	729:733	arg1	angle					743:747	water contact angle	729:747	water contact angle: 154.4°	729:755	Fe3O4 and silylated SiO2 nanoparticles endowed the adsorbent with magnetism (saturation magnetization: 4.36 emu/g) and superhydrophobicity (water contact angle: 154.4°), respectively.					
36007742	5	0	theme	higher	856:861	arg1	adsorption					867:876	higher EBT adsorption	856:876	higher EBT adsorption	856:876	The biocomposite exhibited higher EBT adsorption in the acidic pH range (2-5) due to strong electrostatic and chemical interactions.					
36007742	7	1	theme	biocomposite	1118:1129	arg1	capacity					1106:1113	The maximum adsorption capacity	1083:1113	The maximum adsorption capacity of biocomposite	1083:1129	The maximum adsorption capacity of biocomposite was 876.2 mg/g achieved within 45 min.					
36007742	1	2	theme	engineered	164:173	arg1	hydroxides					208:217	engineered biochar decorated layered double hydroxides	164:217	engineered biochar decorated layered double hydroxides	164:217	In this work, engineered biochar decorated layered double hydroxides and cellulose nanocrystals (B-CuFe-CNC) biocomposites were synthesized by the facile ultrasonicated-co-precipitation technique.					
36007742	10	3	theme	contaminants	1647:1658	arg1	removal					1632:1638	the enhanced removal	1619:1638	the enhanced removal of dye contaminants from wastewater	1619:1674	The results suggest that tailoring biochar properties with layered double hydroxide and CNC is a promising way for the enhanced removal of dye contaminants from wastewater.					
36007742	5	4	theme	EBT	863:865	arg1	adsorption					867:876	higher EBT adsorption	856:876	higher EBT adsorption	856:876	The biocomposite exhibited higher EBT adsorption in the acidic pH range (2-5) due to strong electrostatic and chemical interactions.					
36007742	9	5	theme	high	1386:1389	arg1	removal					1395:1401	high EBT removal	1386:1401	high EBT removal	1386:1401	The biocomposite maintained high EBT removal after six successive adsorption cycles and excellent dye adsorption in the different water matrices.					
36007742	1	6	theme	biochar	175:181	arg1	hydroxides					208:217	engineered biochar decorated layered double hydroxides	164:217	engineered biochar decorated layered double hydroxides	164:217	In this work, engineered biochar decorated layered double hydroxides and cellulose nanocrystals (B-CuFe-CNC) biocomposites were synthesized by the facile ultrasonicated-co-precipitation technique.					
36007742	10	7	from	wastewater	1665:1674	arg1	removal					1632:1638	the enhanced removal	1619:1638	the enhanced removal of dye contaminants from wastewater	1619:1674	The results suggest that tailoring biochar properties with layered double hydroxide and CNC is a promising way for the enhanced removal of dye contaminants from wastewater.					
36007742	0	8	theme	azo	121:123	arg1	performance					137:147	azo dye removal performance	121:147	azo dye removal performance	121:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	8	theme	azo	121:123	arg1	nanocrystals					64:75	layered double hydroxide-cellulose nanocrystals	29:75	layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance	29:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	9	9	theme	successive	1413:1422	arg1	cycles					1435:1440	six successive adsorption cycles	1409:1440	six successive adsorption cycles	1409:1440	The biocomposite maintained high EBT removal after six successive adsorption cycles and excellent dye adsorption in the different water matrices.					
36007742	3	10	with	structure	579:587	arg1	crystallinity					604:616	excellent crystallinity	594:616	excellent crystallinity	594:616	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	10	11	theme	layered	1563:1569	arg1	hydroxide					1578:1586	layered double hydroxide	1563:1586	layered double hydroxide	1563:1586	The results suggest that tailoring biochar properties with layered double hydroxide and CNC is a promising way for the enhanced removal of dye contaminants from wastewater.					
36007742	9	12	theme	adsorption	1424:1433	arg1	cycles					1435:1440	six successive adsorption cycles	1409:1440	six successive adsorption cycles	1409:1440	The biocomposite maintained high EBT removal after six successive adsorption cycles and excellent dye adsorption in the different water matrices.					
36007742	9	13	theme	dye	1456:1458	arg1	adsorption					1460:1469	excellent dye adsorption	1446:1469	excellent dye adsorption	1446:1469	The biocomposite maintained high EBT removal after six successive adsorption cycles and excellent dye adsorption in the different water matrices.					
36007742	3	14	theme	two-dimensional	554:568	arg1	structure					579:587	a two-dimensional rod-like structure	552:587	a two-dimensional rod-like structure with excellent crystallinity	552:616	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	1	15	theme	facile	297:302	arg1	technique					336:344	the facile ultrasonicated-co-precipitation technique	293:344	the facile ultrasonicated-co-precipitation technique	293:344	In this work, engineered biochar decorated layered double hydroxides and cellulose nanocrystals (B-CuFe-CNC) biocomposites were synthesized by the facile ultrasonicated-co-precipitation technique.					
36007742	0	16	theme	removal	129:135	arg1	performance					137:147	azo dye removal performance	121:147	azo dye removal performance	121:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	16	theme	removal	129:135	arg1	nanocrystals					64:75	layered double hydroxide-cellulose nanocrystals	29:75	layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance	29:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	10	17	theme	double	1571:1576	arg1	hydroxide					1578:1586	layered double hydroxide	1563:1586	layered double hydroxide	1563:1586	The results suggest that tailoring biochar properties with layered double hydroxide and CNC is a promising way for the enhanced removal of dye contaminants from wastewater.					
36007742	5	18	theme	due	907:909	arg1	range					895:899	the acidic pH range	881:899	the acidic pH range (2-5) due to strong electrostatic and chemical interactions	881:959	The biocomposite exhibited higher EBT adsorption in the acidic pH range (2-5) due to strong electrostatic and chemical interactions.					
36007742	3	19	theme	double	522:527	arg1	hydroxides					529:538	biochar-layered double hydroxides	506:538	biochar-layered double hydroxides	506:538	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	3	20	theme	excellent	594:602	arg1	crystallinity					604:616	excellent crystallinity	594:616	excellent crystallinity	594:616	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	4	21	theme	adsorption	751:760	arg1	system					762:767	The adsorption system	747:767	The adsorption system	747:767	The adsorption system was appropriately demonstrated by the BBD-RSM (R2 > 0.994).					
36007742	1	22	theme	decorated	183:191	arg1	hydroxides					208:217	engineered biochar decorated layered double hydroxides	164:217	engineered biochar decorated layered double hydroxides	164:217	In this work, engineered biochar decorated layered double hydroxides and cellulose nanocrystals (B-CuFe-CNC) biocomposites were synthesized by the facile ultrasonicated-co-precipitation technique.					
36007742	0	23	theme	dye	125:127	arg1	performance					137:147	azo dye removal performance	121:147	azo dye removal performance	121:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	23	theme	dye	125:127	arg1	nanocrystals					64:75	layered double hydroxide-cellulose nanocrystals	29:75	layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance	29:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	8	24	theme	electrostatic	1270:1282	arg1	attraction					1284:1293	electrostatic attraction	1270:1293	electrostatic attraction	1270:1293	The spectroscopic analyses imply that the high removal of EBT by biocomposite is mainly governed by electrostatic attraction, hydrogen bonding, and chemical/metal complexation mechanisms.					
36007742	1	25	theme	layered	193:199	arg1	hydroxides					208:217	engineered biochar decorated layered double hydroxides	164:217	engineered biochar decorated layered double hydroxides	164:217	In this work, engineered biochar decorated layered double hydroxides and cellulose nanocrystals (B-CuFe-CNC) biocomposites were synthesized by the facile ultrasonicated-co-precipitation technique.					
36007742	5	26	theme	pH	892:893	arg1	range					895:899	the acidic pH range	881:899	the acidic pH range (2-5) due to strong electrostatic and chemical interactions	881:959	The biocomposite exhibited higher EBT adsorption in the acidic pH range (2-5) due to strong electrostatic and chemical interactions.					
36007742	9	27	theme	excellent	1446:1454	arg1	adsorption					1460:1469	excellent dye adsorption	1446:1469	excellent dye adsorption	1446:1469	The biocomposite maintained high EBT removal after six successive adsorption cycles and excellent dye adsorption in the different water matrices.					
36007742	3	28	attach	presence	487:494	arg1	hydroxides					529:538	biochar-layered double hydroxides	506:538	biochar-layered double hydroxides	506:538	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	3	28	attach	presence	487:494	arg2	CNC					499:501	CNC	499:501	CNC	499:501	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	1	29	theme	double	201:206	arg1	hydroxides					208:217	engineered biochar decorated layered double hydroxides	164:217	engineered biochar decorated layered double hydroxides	164:217	In this work, engineered biochar decorated layered double hydroxides and cellulose nanocrystals (B-CuFe-CNC) biocomposites were synthesized by the facile ultrasonicated-co-precipitation technique.					
36007742	0	30	theme	Engineered	0:9	arg1	biochar					11:17	Engineered biochar	0:17	Engineered biochar	0:17	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	1	31	theme	ultrasonicated-co-precipitation	304:334	arg1	technique					336:344	the facile ultrasonicated-co-precipitation technique	293:344	the facile ultrasonicated-co-precipitation technique	293:344	In this work, engineered biochar decorated layered double hydroxides and cellulose nanocrystals (B-CuFe-CNC) biocomposites were synthesized by the facile ultrasonicated-co-precipitation technique.					
36007742	9	32	from	adsorption	1460:1469	arg1	matrices					1494:1501	the different water matrices	1474:1501	the different water matrices	1474:1501	The biocomposite maintained high EBT removal after six successive adsorption cycles and excellent dye adsorption in the different water matrices.					
36007742	5	33	theme	electrostatic	921:933	arg1	interactions					948:959	strong electrostatic and chemical interactions	914:959	strong electrostatic and chemical interactions	914:959	The biocomposite exhibited higher EBT adsorption in the acidic pH range (2-5) due to strong electrostatic and chemical interactions.					
36007742	10	34	theme	promising	1601:1609	arg1	way					1611:1613	a promising way	1599:1613	a promising way for the enhanced removal of dye contaminants from wastewater	1599:1674	The results suggest that tailoring biochar properties with layered double hydroxide and CNC is a promising way for the enhanced removal of dye contaminants from wastewater.					
36007742	2	35	theme	dye	426:428	arg1	purification					385:396	purification	385:396	purification of Eriochrome Black T (EBT) dye from water	385:439	The biocomposite was investigated for purification of Eriochrome Black T (EBT) dye from water.					
36007742	0	36	theme	layered	29:35	arg1	performance					137:147	azo dye removal performance	121:147	azo dye removal performance	121:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	36	theme	layered	29:35	arg1	characterization					100:115	characterization	100:115	characterization	100:115	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	36	theme	layered	29:35	arg1	nanocrystals					64:75	layered double hydroxide-cellulose nanocrystals	29:75	layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance	29:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	36	theme	layered	29:35	arg1	Synthesis					89:97	Synthesis	89:97	Synthesis	89:97	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	10	37	theme	biochar	1539:1545	arg1	properties					1547:1556	biochar properties	1539:1556	biochar properties	1539:1556	The results suggest that tailoring biochar properties with layered double hydroxide and CNC is a promising way for the enhanced removal of dye contaminants from wastewater.					
36007742	8	38	theme	spectroscopic	1174:1186	arg1	analyses					1188:1195	The spectroscopic analyses	1170:1195	The spectroscopic analyses	1170:1195	The spectroscopic analyses imply that the high removal of EBT by biocomposite is mainly governed by electrostatic attraction, hydrogen bonding, and chemical/metal complexation mechanisms.					
36007742	8	39	theme	hydrogen	1296:1303	arg1	bonding					1305:1311	hydrogen bonding	1296:1311	hydrogen bonding	1296:1311	The spectroscopic analyses imply that the high removal of EBT by biocomposite is mainly governed by electrostatic attraction, hydrogen bonding, and chemical/metal complexation mechanisms.					
36007742	3	40	theme	surface	628:634	arg1	functionalities					636:650	surface functionalities	628:650	surface functionalities	628:650	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	3	41	theme	biochar-layered	506:520	arg1	hydroxides					529:538	biochar-layered double hydroxides	506:538	biochar-layered double hydroxides	506:538	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	9	42	from	cycles	1435:1440	arg1	matrices					1494:1501	the different water matrices	1474:1501	the different water matrices	1474:1501	The biocomposite maintained high EBT removal after six successive adsorption cycles and excellent dye adsorption in the different water matrices.					
36007742	3	43	theme	anionic	724:730	arg1	molecules					736:744	azo anionic dye molecules	720:744	azo anionic dye molecules	720:744	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	2	44	theme	T	418:418	arg1	dye					426:428	Eriochrome Black T (EBT) dye	401:428	Eriochrome Black T (EBT) dye	401:428	The biocomposite was investigated for purification of Eriochrome Black T (EBT) dye from water.					
36007742	1	45	theme	cellulose	223:231	arg1	biocomposites					259:271	cellulose nanocrystals (B-CuFe-CNC) biocomposites	223:271	cellulose nanocrystals (B-CuFe-CNC) biocomposites	223:271	In this work, engineered biochar decorated layered double hydroxides and cellulose nanocrystals (B-CuFe-CNC) biocomposites were synthesized by the facile ultrasonicated-co-precipitation technique.					
36007742	0	46	theme	hydroxide-cellulose	44:62	arg1	performance					137:147	azo dye removal performance	121:147	azo dye removal performance	121:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	46	theme	hydroxide-cellulose	44:62	arg1	characterization					100:115	characterization	100:115	characterization	100:115	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	46	theme	hydroxide-cellulose	44:62	arg1	nanocrystals					64:75	layered double hydroxide-cellulose nanocrystals	29:75	layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance	29:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	46	theme	hydroxide-cellulose	44:62	arg1	Synthesis					89:97	Synthesis	89:97	Synthesis	89:97	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	3	47	theme	dye	732:734	arg1	molecules					736:744	azo anionic dye molecules	720:744	azo anionic dye molecules	720:744	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	3	48	theme	attractive	669:678	arg1	platform					680:687	an attractive platform	666:687	an attractive platform for the enhanced adsorption of azo anionic dye molecules	666:744	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	1	49	theme	nanocrystals	233:244	arg1	biocomposites					259:271	cellulose nanocrystals (B-CuFe-CNC) biocomposites	223:271	cellulose nanocrystals (B-CuFe-CNC) biocomposites	223:271	In this work, engineered biochar decorated layered double hydroxides and cellulose nanocrystals (B-CuFe-CNC) biocomposites were synthesized by the facile ultrasonicated-co-precipitation technique.					
36007742	0	50	theme	double	37:42	arg1	performance					137:147	azo dye removal performance	121:147	azo dye removal performance	121:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	50	theme	double	37:42	arg1	characterization					100:115	characterization	100:115	characterization	100:115	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	50	theme	double	37:42	arg1	nanocrystals					64:75	layered double hydroxide-cellulose nanocrystals	29:75	layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance	29:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	50	theme	double	37:42	arg1	Synthesis					89:97	Synthesis	89:97	Synthesis	89:97	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	9	51	theme	different	1478:1486	arg1	matrices					1494:1501	the different water matrices	1474:1501	the different water matrices	1474:1501	The biocomposite maintained high EBT removal after six successive adsorption cycles and excellent dye adsorption in the different water matrices.					
36007742	5	52	theme	chemical	939:946	arg1	interactions					948:959	strong electrostatic and chemical interactions	914:959	strong electrostatic and chemical interactions	914:959	The biocomposite exhibited higher EBT adsorption in the acidic pH range (2-5) due to strong electrostatic and chemical interactions.					
36007742	10	53	theme	enhanced	1623:1630	arg1	removal					1632:1638	the enhanced removal	1619:1638	the enhanced removal of dye contaminants from wastewater	1619:1674	The results suggest that tailoring biochar properties with layered double hydroxide and CNC is a promising way for the enhanced removal of dye contaminants from wastewater.					
36007742	0	54	dep	nanocrystals	64:75	arg1	performance					137:147	azo dye removal performance	121:147	azo dye removal performance	121:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	54	dep	nanocrystals	64:75	arg1	characterization					100:115	characterization	100:115	characterization	100:115	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	54	dep	nanocrystals	64:75	arg1	nanocrystals					64:75	layered double hydroxide-cellulose nanocrystals	29:75	layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance	29:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	54	dep	nanocrystals	64:75	arg1	Synthesis					89:97	Synthesis	89:97	Synthesis	89:97	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	7	55	theme	adsorption	1095:1104	arg1	capacity					1106:1113	The maximum adsorption capacity	1083:1113	The maximum adsorption capacity of biocomposite	1083:1129	The maximum adsorption capacity of biocomposite was 876.2 mg/g achieved within 45 min.					
36007742	5	56	theme	strong	914:919	arg1	interactions					948:959	strong electrostatic and chemical interactions	914:959	strong electrostatic and chemical interactions	914:959	The biocomposite exhibited higher EBT adsorption in the acidic pH range (2-5) due to strong electrostatic and chemical interactions.					
36007742	0	57	theme	composite-	77:86	arg1	performance					137:147	azo dye removal performance	121:147	azo dye removal performance	121:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	57	theme	composite-	77:86	arg1	characterization					100:115	characterization	100:115	characterization	100:115	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	57	theme	composite-	77:86	arg1	nanocrystals					64:75	layered double hydroxide-cellulose nanocrystals	29:75	layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance	29:147	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	0	57	theme	composite-	77:86	arg1	Synthesis					89:97	Synthesis	89:97	Synthesis	89:97	Engineered biochar supported layered double hydroxide-cellulose nanocrystals composite-: Synthesis, characterization and azo dye removal performance.					
36007742	9	58	theme	water	1488:1492	arg1	matrices					1494:1501	the different water matrices	1474:1501	the different water matrices	1474:1501	The biocomposite maintained high EBT removal after six successive adsorption cycles and excellent dye adsorption in the different water matrices.					
36007742	5	59	theme	acidic	885:890	arg1	range					895:899	the acidic pH range	881:899	the acidic pH range (2-5) due to strong electrostatic and chemical interactions	881:959	The biocomposite exhibited higher EBT adsorption in the acidic pH range (2-5) due to strong electrostatic and chemical interactions.					
36007742	3	60	theme	azo	720:722	arg1	molecules					736:744	azo anionic dye molecules	720:744	azo anionic dye molecules	720:744	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	2	61	theme	Black	412:416	arg1	dye					426:428	Eriochrome Black T (EBT) dye	401:428	Eriochrome Black T (EBT) dye	401:428	The biocomposite was investigated for purification of Eriochrome Black T (EBT) dye from water.					
36007742	3	62	theme	characterization	446:461	arg1	results					463:469	The characterization results	442:469	The characterization results	442:469	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	10	63	theme	dye	1643:1645	arg1	contaminants					1647:1658	dye contaminants	1643:1658	dye contaminants	1643:1658	The results suggest that tailoring biochar properties with layered double hydroxide and CNC is a promising way for the enhanced removal of dye contaminants from wastewater.					
36007742	6	64	dep	Freundlich	1042:1051	arg1	models					1075:1080	models	1075:1080	models	1075:1080	The kinetic and isotherm results were well demonstrated by pseudo-second order, Freundlich, and Redlich Peterson models.					
36007742	3	65	theme	rod-like	570:577	arg1	structure					579:587	a two-dimensional rod-like structure	552:587	a two-dimensional rod-like structure with excellent crystallinity	552:616	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	2	66	theme	Eriochrome	401:410	arg1	dye					426:428	Eriochrome Black T (EBT) dye	401:428	Eriochrome Black T (EBT) dye	401:428	The biocomposite was investigated for purification of Eriochrome Black T (EBT) dye from water.					
36007742	3	67	theme	enhanced	697:704	arg1	adsorption					706:715	the enhanced adsorption	693:715	the enhanced adsorption of azo anionic dye molecules	693:744	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	2	68	from	water	435:439	arg1	purification					385:396	purification	385:396	purification of Eriochrome Black T (EBT) dye from water	385:439	The biocomposite was investigated for purification of Eriochrome Black T (EBT) dye from water.					
36007742	1	69	theme	B-CuFe-CNC	247:256	arg1	biocomposites					259:271	cellulose nanocrystals (B-CuFe-CNC) biocomposites	223:271	cellulose nanocrystals (B-CuFe-CNC) biocomposites	223:271	In this work, engineered biochar decorated layered double hydroxides and cellulose nanocrystals (B-CuFe-CNC) biocomposites were synthesized by the facile ultrasonicated-co-precipitation technique.					
36007742	7	70	theme	maximum	1087:1093	arg1	capacity					1106:1113	The maximum adsorption capacity	1083:1113	The maximum adsorption capacity of biocomposite	1083:1129	The maximum adsorption capacity of biocomposite was 876.2 mg/g achieved within 45 min.					
36007742	3	71	theme	molecules	736:744	arg1	adsorption					706:715	the enhanced adsorption	693:715	the enhanced adsorption of azo anionic dye molecules	693:744	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	8	72	theme	EBT	1228:1230	arg1	removal					1217:1223	the high removal	1208:1223	the high removal of EBT by biocomposite	1208:1246	The spectroscopic analyses imply that the high removal of EBT by biocomposite is mainly governed by electrostatic attraction, hydrogen bonding, and chemical/metal complexation mechanisms.					
36007742	9	73	theme	EBT	1391:1393	arg1	removal					1395:1401	high EBT removal	1386:1401	high EBT removal	1386:1401	The biocomposite maintained high EBT removal after six successive adsorption cycles and excellent dye adsorption in the different water matrices.					
36007742	3	74	theme	CNC	499:501	arg1	presence					487:494	the presence	483:494	the presence of CNC in biochar-layered double hydroxides	483:538	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	8	75	theme	complexation	1333:1344	arg1	mechanisms					1346:1355	chemical/metal complexation mechanisms	1318:1355	chemical/metal complexation mechanisms	1318:1355	The spectroscopic analyses imply that the high removal of EBT by biocomposite is mainly governed by electrostatic attraction, hydrogen bonding, and chemical/metal complexation mechanisms.					
36007742	8	76	theme	chemical/metal	1318:1331	arg1	mechanisms					1346:1355	chemical/metal complexation mechanisms	1318:1355	chemical/metal complexation mechanisms	1318:1355	The spectroscopic analyses imply that the high removal of EBT by biocomposite is mainly governed by electrostatic attraction, hydrogen bonding, and chemical/metal complexation mechanisms.					
36007742	3	77	from	presence	487:494	arg1	hydroxides					529:538	biochar-layered double hydroxides	506:538	biochar-layered double hydroxides	506:538	The characterization results showed that the presence of CNC in biochar-layered double hydroxides resulted in a two-dimensional rod-like structure with excellent crystallinity, improved surface functionalities, and provides an attractive platform for the enhanced adsorption of azo anionic dye molecules.					
36007742	2	78	theme	EBT	421:423	arg1	dye					426:428	Eriochrome Black T (EBT) dye	401:428	Eriochrome Black T (EBT) dye	401:428	The biocomposite was investigated for purification of Eriochrome Black T (EBT) dye from water.					
36007742	8	79	theme	high	1212:1215	arg1	removal					1217:1223	the high removal	1208:1223	the high removal of EBT by biocomposite	1208:1246	The spectroscopic analyses imply that the high removal of EBT by biocomposite is mainly governed by electrostatic attraction, hydrogen bonding, and chemical/metal complexation mechanisms.					
36007742	6	80	dep	kinetic	966:972	arg1	results					987:993	results	987:993	results	987:993	The kinetic and isotherm results were well demonstrated by pseudo-second order, Freundlich, and Redlich Peterson models.					
36047700	16	0	theme	intestinal	2870:2879	arg1	mixing					2881:2886	intestinal mixing	2870:2886	intestinal mixing	2870:2886	The framework allowed us to test a variety of controlling factors, including intestinal mixing and transit time.					
36047700	4	1	theme	pathogenic	604:613	arg1	competitors					615:625	pathogenic competitors	604:625	pathogenic competitors	604:625	An early transition to Bifidobacterium species is associated with health benefits; for example, Bifidobacterium species repress growth of pathogenic competitors and modulate the immune response.					
36047700	7	2	theme	species	1208:1214	arg1	competition					1156:1166	the competition	1152:1166	the competition of strictly and facultatively anaerobic species in a gut-like environment	1152:1240	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	0	3	from	Aerobic	58:64	arg1	Switch					46:51	a Switch	44:51	a Switch from Aerobic to Anaerobic Metabolism	44:88	A Multiscale Spatiotemporal Model Including a Switch from Aerobic to Anaerobic Metabolism Reproduces Succession in the Early Infant Gut Microbiota.					
36047700	13	4	theme	mucus	2295:2299	arg1	composition					2301:2311	intestinal mucus composition	2284:2311	intestinal mucus composition	2284:2311	Controlling factors could include nutritional composition and intestinal mucus composition, as well as environmental factors, such as antibiotics.					
36047700	10	5	theme	hypotheses	1823:1832	arg1	plausibility					1807:1818	the internal plausibility	1794:1818	the internal plausibility of hypotheses for bacterial colonization and succession in the infant colon	1794:1894	The computational model thus allows us to test the internal plausibility of hypotheses for bacterial colonization and succession in the infant colon.					
36047700	1	6	theme	host	258:261	arg1	health					244:249	the health	240:249	the health of the host	240:261	The human intestinal microbiota starts to form immediately after birth and is important for the health of the host.					
36047700	6	7	theme	mathematical	943:954	arg1	model					956:960	a multiscale mathematical model	930:960	a multiscale mathematical model that considers metabolism, spatial bacterial population dynamics, and cross-feeding	930:1044	To study if oxygen depletion suffices for the transition to Bifidobacterium species, here we introduced a multiscale mathematical model that considers metabolism, spatial bacterial population dynamics, and cross-feeding.					
36047700	7	8	theme	strictly	1171:1178	arg1	species					1208:1214	strictly and facultatively anaerobic species	1171:1214	strictly and facultatively anaerobic species	1171:1214	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	6	9	theme	oxygen	838:843	arg1	depletion					845:853	oxygen depletion	838:853	oxygen depletion	838:853	To study if oxygen depletion suffices for the transition to Bifidobacterium species, here we introduced a multiscale mathematical model that considers metabolism, spatial bacterial population dynamics, and cross-feeding.					
36047700	8	10	theme	intracolonic	1335:1346	arg1	oxygen					1348:1353	intracolonic oxygen	1335:1353	intracolonic oxygen in newborn infants	1335:1372	The model predicts that individual differences in intracolonic oxygen in newborn infants can explain the observed individual variation in succession to anaerobic species, in particular Bifidobacterium species.					
36047700	0	11	theme	Early	119:123	arg1	Microbiota					136:145	the Early Infant Gut Microbiota	115:145	the Early Infant Gut Microbiota	115:145	A Multiscale Spatiotemporal Model Including a Switch from Aerobic to Anaerobic Metabolism Reproduces Succession in the Early Infant Gut Microbiota.					
36047700	9	12	theme	bifid	1568:1572	arg1	shunt					1574:1578	the bifid shunt	1564:1578	the bifid shunt	1564:1578	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	11	13	theme	microbiota	1938:1947	arg1	composition					1912:1922	The composition	1908:1922	IMPORTANCE The composition of the infant microbiota	1897:1947	IMPORTANCE The composition of the infant microbiota has a great impact on infant health, but its controlling factors are still incompletely understood.					
36047700	0	14	theme	Gut	132:134	arg1	Microbiota					136:145	the Early Infant Gut Microbiota	115:145	the Early Infant Gut Microbiota	115:145	A Multiscale Spatiotemporal Model Including a Switch from Aerobic to Anaerobic Metabolism Reproduces Succession in the Early Infant Gut Microbiota.					
36047700	5	15	from	newborns	761:768	arg1	consumption					715:725	consumption	715:725	consumption of intracolonic oxygen present in newborns by facultative anaerobes, including Enterobacteriaceae	715:823	Succession to Bifidobacterium is thought to be due to consumption of intracolonic oxygen present in newborns by facultative anaerobes, including Enterobacteriaceae.					
36047700	15	16	theme	species	2661:2667	arg1	dominance					2632:2640	the dominance	2628:2640	the dominance of Bifidobacterium species	2628:2667	We present simulations that suggest that greater levels of intraintestinal oxygenation more strongly delay the dominance of Bifidobacterium species, explaining the observed variety of microbial composition and demonstrating the use of the model for hypothesis generation.					
36047700	12	17	theme	anaerobic	2073:2081	arg1	species					2099:2105	The frequently dominant anaerobic Bifidobacterium species	2049:2105	The frequently dominant anaerobic Bifidobacterium species benefit health	2049:2120	The frequently dominant anaerobic Bifidobacterium species benefit health, e.g., they can keep harmful competitors under control and modulate the intestinal immune response.					
36047700	6	18	theme	population	1007:1016	arg1	dynamics					1018:1025	spatial bacterial population dynamics	989:1025	spatial bacterial population dynamics	989:1025	To study if oxygen depletion suffices for the transition to Bifidobacterium species, here we introduced a multiscale mathematical model that considers metabolism, spatial bacterial population dynamics, and cross-feeding.					
36047700	4	19	theme	early	469:473	arg1	transition					475:484	An early transition	466:484	An early transition to Bifidobacterium species	466:511	An early transition to Bifidobacterium species is associated with health benefits; for example, Bifidobacterium species repress growth of pathogenic competitors and modulate the immune response.					
36047700	12	20	theme	intestinal	2194:2203	arg1	response					2212:2219	the intestinal immune response	2190:2219	the intestinal immune response	2190:2219	The frequently dominant anaerobic Bifidobacterium species benefit health, e.g., they can keep harmful competitors under control and modulate the intestinal immune response.					
36047700	7	21	theme	network	1082:1088	arg1	data					1090:1093	publicly available metabolic network data	1053:1093	publicly available metabolic network data from the AGORA collection	1053:1119	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	6	22	theme	spatial	989:995	arg1	dynamics					1018:1025	spatial bacterial population dynamics	989:1025	spatial bacterial population dynamics	989:1025	To study if oxygen depletion suffices for the transition to Bifidobacterium species, here we introduced a multiscale mathematical model that considers metabolism, spatial bacterial population dynamics, and cross-feeding.					
36047700	8	23	theme	observed	1390:1397	arg1	variation					1410:1418	the observed individual variation	1386:1418	the observed individual variation in succession to anaerobic species	1386:1453	The model predicts that individual differences in intracolonic oxygen in newborn infants can explain the observed individual variation in succession to anaerobic species, in particular Bifidobacterium species.					
36047700	7	24	theme	available	1062:1070	arg1	data					1090:1093	publicly available metabolic network data	1053:1093	publicly available metabolic network data from the AGORA collection	1053:1119	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	17	25	theme	metabolism	2987:2996	arg1	modeling					2949:2956	detailed modeling	2940:2956	detailed modeling of oligosaccharide and mucin metabolism	2940:2996	Future versions will also include detailed modeling of oligosaccharide and mucin metabolism.					
36047700	10	26	from	colonization	1848:1859	arg1	colon					1890:1894	the infant colon	1879:1894	the infant colon	1879:1894	The computational model thus allows us to test the internal plausibility of hypotheses for bacterial colonization and succession in the infant colon.					
36047700	11	27	dep	IMPORTANCE	1897:1906	arg1	composition					1912:1922	The composition	1908:1922	IMPORTANCE The composition of the infant microbiota	1897:1947	IMPORTANCE The composition of the infant microbiota has a great impact on infant health, but its controlling factors are still incompletely understood.					
36047700	0	28	theme	Anaerobic	69:77	arg1	Metabolism					79:88	Anaerobic Metabolism	69:88	Anaerobic Metabolism	69:88	A Multiscale Spatiotemporal Model Including a Switch from Aerobic to Anaerobic Metabolism Reproduces Succession in the Early Infant Gut Microbiota.					
36047700	15	29	theme	composition	2715:2725	arg1	variety					2694:2700	the observed variety	2681:2700	the observed variety of microbial composition	2681:2725	We present simulations that suggest that greater levels of intraintestinal oxygenation more strongly delay the dominance of Bifidobacterium species, explaining the observed variety of microbial composition and demonstrating the use of the model for hypothesis generation.					
36047700	15	29	theme	composition	2715:2725	arg1	composition					2715:2725	microbial composition	2705:2725	microbial composition	2705:2725	We present simulations that suggest that greater levels of intraintestinal oxygenation more strongly delay the dominance of Bifidobacterium species, explaining the observed variety of microbial composition and demonstrating the use of the model for hypothesis generation.					
36047700	1	30	theme	intestinal	158:167	arg1	microbiota					169:178	The human intestinal microbiota	148:178	The human intestinal microbiota	148:178	The human intestinal microbiota starts to form immediately after birth and is important for the health of the host.					
36047700	1	30	theme	intestinal	158:167	arg1	important					226:234	important	226:234	important	226:234	The human intestinal microbiota starts to form immediately after birth and is important for the health of the host.					
36047700	4	31	theme	health	532:537	arg1	benefits					539:546	health benefits	532:546	health benefits	532:546	An early transition to Bifidobacterium species is associated with health benefits; for example, Bifidobacterium species repress growth of pathogenic competitors and modulate the immune response.					
36047700	8	32	theme	anaerobic	1437:1445	arg1	species					1447:1453	anaerobic species	1437:1453	anaerobic species	1437:1453	The model predicts that individual differences in intracolonic oxygen in newborn infants can explain the observed individual variation in succession to anaerobic species, in particular Bifidobacterium species.					
36047700	14	33	theme	microbial	2443:2451	arg1	ecology					2453:2459	a metabolically realistic intestinal microbial ecology	2406:2459	a metabolically realistic intestinal microbial ecology in which hypothetical scenarios can be tested and compared	2406:2518	We introduce a modeling framework of a metabolically realistic intestinal microbial ecology in which hypothetical scenarios can be tested and compared.					
36047700	15	34	theme	greater	2562:2568	arg1	levels					2570:2575	greater levels	2562:2575	greater levels of intraintestinal oxygenation	2562:2606	We present simulations that suggest that greater levels of intraintestinal oxygenation more strongly delay the dominance of Bifidobacterium species, explaining the observed variety of microbial composition and demonstrating the use of the model for hypothesis generation.					
36047700	14	35	theme	realistic	2422:2430	arg1	ecology					2453:2459	a metabolically realistic intestinal microbial ecology	2406:2459	a metabolically realistic intestinal microbial ecology in which hypothetical scenarios can be tested and compared	2406:2518	We introduce a modeling framework of a metabolically realistic intestinal microbial ecology in which hypothetical scenarios can be tested and compared.					
36047700	3	36	theme	Bifidobacterium	441:455	arg1	species					457:463	Bifidobacterium species	441:463	Bifidobacterium species	441:463	These are succeeded by strictly anaerobic species, particularly Bifidobacterium species.					
36047700	3	36	theme	Bifidobacterium	441:455	arg1	species					419:425	strictly anaerobic species	400:425	strictly anaerobic species	400:425	These are succeeded by strictly anaerobic species, particularly Bifidobacterium species.					
36047700	15	37	theme	observed	2685:2692	arg1	variety					2694:2700	the observed variety	2681:2700	the observed variety of microbial composition	2681:2725	We present simulations that suggest that greater levels of intraintestinal oxygenation more strongly delay the dominance of Bifidobacterium species, explaining the observed variety of microbial composition and demonstrating the use of the model for hypothesis generation.					
36047700	15	37	theme	observed	2685:2692	arg1	composition					2715:2725	microbial composition	2705:2725	microbial composition	2705:2725	We present simulations that suggest that greater levels of intraintestinal oxygenation more strongly delay the dominance of Bifidobacterium species, explaining the observed variety of microbial composition and demonstrating the use of the model for hypothesis generation.					
36047700	12	38	dep	species	2099:2105	arg1	benefit					2107:2113	benefit	2107:2113	benefit health	2107:2120	The frequently dominant anaerobic Bifidobacterium species benefit health, e.g., they can keep harmful competitors under control and modulate the intestinal immune response.					
36047700	8	39	theme	particular	1459:1468	arg1	species					1486:1492	particular Bifidobacterium species	1459:1492	particular Bifidobacterium species	1459:1492	The model predicts that individual differences in intracolonic oxygen in newborn infants can explain the observed individual variation in succession to anaerobic species, in particular Bifidobacterium species.					
36047700	8	40	from	variation	1410:1418	arg1	succession					1423:1432	succession	1423:1432	succession	1423:1432	The model predicts that individual differences in intracolonic oxygen in newborn infants can explain the observed individual variation in succession to anaerobic species, in particular Bifidobacterium species.					
36047700	9	41	theme	high	1671:1674	arg1	concentrations					1684:1697	high lactose concentrations	1671:1697	high lactose concentrations	1671:1697	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	16	42	theme	transit	2892:2898	arg1	time					2900:2903	transit time	2892:2903	transit time	2892:2903	The framework allowed us to test a variety of controlling factors, including intestinal mixing and transit time.					
36047700	17	43	theme	detailed	2940:2947	arg1	modeling					2949:2956	detailed modeling	2940:2956	detailed modeling of oligosaccharide and mucin metabolism	2940:2996	Future versions will also include detailed modeling of oligosaccharide and mucin metabolism.					
36047700	4	44	theme	Bifidobacterium	562:576	arg1	species					578:584	Bifidobacterium species	562:584	Bifidobacterium species	562:584	An early transition to Bifidobacterium species is associated with health benefits; for example, Bifidobacterium species repress growth of pathogenic competitors and modulate the immune response.					
36047700	0	45	theme	Spatiotemporal	13:26	arg1	Model					28:32	A Multiscale Spatiotemporal Model	0:32	A Multiscale Spatiotemporal Model Including a Switch from Aerobic to Anaerobic Metabolism	0:88	A Multiscale Spatiotemporal Model Including a Switch from Aerobic to Anaerobic Metabolism Reproduces Succession in the Early Infant Gut Microbiota.					
36047700	9	46	from	model	1542:1546	arg1	dominant					1526:1533	dominant	1526:1533	dominant	1526:1533	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	13	47	theme	intestinal	2284:2293	arg1	composition					2301:2311	intestinal mucus composition	2284:2311	intestinal mucus composition	2284:2311	Controlling factors could include nutritional composition and intestinal mucus composition, as well as environmental factors, such as antibiotics.					
36047700	3	48	theme	anaerobic	409:417	arg1	species					457:463	Bifidobacterium species	441:463	Bifidobacterium species	441:463	These are succeeded by strictly anaerobic species, particularly Bifidobacterium species.					
36047700	3	48	theme	anaerobic	409:417	arg1	species					419:425	strictly anaerobic species	400:425	strictly anaerobic species	400:425	These are succeeded by strictly anaerobic species, particularly Bifidobacterium species.					
36047700	14	49	theme	hypothetical	2470:2481	arg1	scenarios					2483:2491	hypothetical scenarios	2470:2491	hypothetical scenarios	2470:2491	We introduce a modeling framework of a metabolically realistic intestinal microbial ecology in which hypothetical scenarios can be tested and compared.					
36047700	15	50	theme	oxygenation	2596:2606	arg1	levels					2570:2575	greater levels	2562:2575	greater levels of intraintestinal oxygenation	2562:2606	We present simulations that suggest that greater levels of intraintestinal oxygenation more strongly delay the dominance of Bifidobacterium species, explaining the observed variety of microbial composition and demonstrating the use of the model for hypothesis generation.					
36047700	11	51	contain	has	1949:1951	arg1	IMPORTANCE					1897:1906	IMPORTANCE	1897:1906	IMPORTANCE The composition of the infant microbiota	1897:1947	IMPORTANCE The composition of the infant microbiota has a great impact on infant health, but its controlling factors are still incompletely understood.					
36047700	11	51	contain	has	1949:1951	arg2	impact					1961:1966	a great impact	1953:1966	a great impact on infant health	1953:1983	IMPORTANCE The composition of the infant microbiota has a great impact on infant health, but its controlling factors are still incompletely understood.					
36047700	15	52	theme	hypothesis	2770:2779	arg1	generation					2781:2790	hypothesis generation	2770:2790	hypothesis generation	2770:2790	We present simulations that suggest that greater levels of intraintestinal oxygenation more strongly delay the dominance of Bifidobacterium species, explaining the observed variety of microbial composition and demonstrating the use of the model for hypothesis generation.					
36047700	5	53	from	consumption	715:725	arg1	newborns					761:768	newborns	761:768	newborns	761:768	Succession to Bifidobacterium is thought to be due to consumption of intracolonic oxygen present in newborns by facultative anaerobes, including Enterobacteriaceae.					
36047700	9	54	theme	flux	1724:1727	arg1	analysis					1737:1744	flux balance analysis	1724:1744	flux balance analysis	1724:1744	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	7	55	from	collection	1110:1119	arg1	data					1090:1093	publicly available metabolic network data	1053:1093	publicly available metabolic network data from the AGORA collection	1053:1119	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	10	56	theme	bacterial	1838:1846	arg1	colonization					1848:1859	bacterial colonization	1838:1859	bacterial colonization	1838:1859	The computational model thus allows us to test the internal plausibility of hypotheses for bacterial colonization and succession in the infant colon.					
36047700	15	57	theme	model	2760:2764	arg1	use					2749:2751	the use	2745:2751	the use of the model for hypothesis generation	2745:2790	We present simulations that suggest that greater levels of intraintestinal oxygenation more strongly delay the dominance of Bifidobacterium species, explaining the observed variety of microbial composition and demonstrating the use of the model for hypothesis generation.					
36047700	7	58	theme	lactose	1265:1271	arg1	influence					1252:1260	the influence	1248:1260	the influence of lactose and oxygen	1248:1282	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	16	59	theme	factors	2851:2857	arg1	mixing					2881:2886	intestinal mixing	2870:2886	intestinal mixing	2870:2886	The framework allowed us to test a variety of controlling factors, including intestinal mixing and transit time.					
36047700	16	59	theme	factors	2851:2857	arg1	time					2900:2903	transit time	2892:2903	transit time	2892:2903	The framework allowed us to test a variety of controlling factors, including intestinal mixing and transit time.					
36047700	16	59	theme	factors	2851:2857	arg1	variety					2828:2834	a variety	2826:2834	a variety of controlling factors, including intestinal mixing and transit time	2826:2903	The framework allowed us to test a variety of controlling factors, including intestinal mixing and transit time.					
36047700	16	59	theme	factors	2851:2857	arg1	factors					2851:2857	controlling factors	2839:2857	controlling factors	2839:2857	The framework allowed us to test a variety of controlling factors, including intestinal mixing and transit time.					
36047700	4	60	theme	immune	644:649	arg1	response					651:658	the immune response	640:658	the immune response	640:658	An early transition to Bifidobacterium species is associated with health benefits; for example, Bifidobacterium species repress growth of pathogenic competitors and modulate the immune response.					
36047700	7	61	theme	anaerobic	1198:1206	arg1	species					1208:1214	strictly and facultatively anaerobic species	1171:1214	strictly and facultatively anaerobic species	1171:1214	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	11	62	theme	infant	1971:1976	arg1	health					1978:1983	infant health	1971:1983	infant health	1971:1983	IMPORTANCE The composition of the infant microbiota has a great impact on infant health, but its controlling factors are still incompletely understood.					
36047700	5	63	from	present	750:756	arg1	newborns					761:768	newborns	761:768	newborns	761:768	Succession to Bifidobacterium is thought to be due to consumption of intracolonic oxygen present in newborns by facultative anaerobes, including Enterobacteriaceae.					
36047700	12	64	theme	harmful	2143:2149	arg1	competitors					2151:2161	harmful competitors	2143:2161	harmful competitors	2143:2161	The frequently dominant anaerobic Bifidobacterium species benefit health, e.g., they can keep harmful competitors under control and modulate the intestinal immune response.					
36047700	2	65	theme	first	275:279	arg1	days					281:284	the first days	271:284	the first days	271:284	During the first days, facultatively anaerobic bacterial species generally dominate, such as Enterobacteriaceae.					
36047700	9	66	theme	suboptimal	1623:1632	arg1	metabolism					1640:1649	suboptimal yield metabolism	1623:1649	suboptimal yield metabolism	1623:1649	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	4	67	theme	competitors	615:625	arg1	growth					594:599	growth	594:599	growth of pathogenic competitors	594:625	An early transition to Bifidobacterium species is associated with health benefits; for example, Bifidobacterium species repress growth of pathogenic competitors and modulate the immune response.					
36047700	6	68	theme	multiscale	932:941	arg1	model					956:960	a multiscale mathematical model	930:960	a multiscale mathematical model that considers metabolism, spatial bacterial population dynamics, and cross-feeding	930:1044	To study if oxygen depletion suffices for the transition to Bifidobacterium species, here we introduced a multiscale mathematical model that considers metabolism, spatial bacterial population dynamics, and cross-feeding.					
36047700	17	69	theme	Future	2906:2911	arg1	versions					2913:2920	Future versions	2906:2920	Future versions	2906:2920	Future versions will also include detailed modeling of oligosaccharide and mucin metabolism.					
36047700	8	70	theme	individual	1309:1318	arg1	differences					1320:1330	individual differences	1309:1330	individual differences in intracolonic oxygen in newborn infants	1309:1372	The model predicts that individual differences in intracolonic oxygen in newborn infants can explain the observed individual variation in succession to anaerobic species, in particular Bifidobacterium species.					
36047700	2	71	theme	anaerobic	301:309	arg1	species					321:327	facultatively anaerobic bacterial species	287:327	facultatively anaerobic bacterial species	287:327	During the first days, facultatively anaerobic bacterial species generally dominate, such as Enterobacteriaceae.					
36047700	12	72	theme	Bifidobacterium	2083:2097	arg1	species					2099:2105	The frequently dominant anaerobic Bifidobacterium species	2049:2105	The frequently dominant anaerobic Bifidobacterium species benefit health	2049:2120	The frequently dominant anaerobic Bifidobacterium species benefit health, e.g., they can keep harmful competitors under control and modulate the intestinal immune response.					
36047700	7	73	theme	ab	1142:1143	arg1	initio					1145:1150	ab initio	1142:1150	ab initio	1142:1150	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	10	74	theme	infant	1883:1888	arg1	colon					1890:1894	the infant colon	1879:1894	the infant colon	1879:1894	The computational model thus allows us to test the internal plausibility of hypotheses for bacterial colonization and succession in the infant colon.					
36047700	9	75	theme	yield	1634:1638	arg1	metabolism					1640:1649	suboptimal yield metabolism	1623:1649	suboptimal yield metabolism	1623:1649	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	5	76	theme	present	750:756	arg1	consumption					715:725	consumption	715:725	consumption of intracolonic oxygen present in newborns by facultative anaerobes, including Enterobacteriaceae	715:823	Succession to Bifidobacterium is thought to be due to consumption of intracolonic oxygen present in newborns by facultative anaerobes, including Enterobacteriaceae.					
36047700	13	77	theme	nutritional	2256:2266	arg1	composition					2268:2278	nutritional composition	2256:2278	nutritional composition	2256:2278	Controlling factors could include nutritional composition and intestinal mucus composition, as well as environmental factors, such as antibiotics.					
36047700	9	78	theme	shunt	1574:1578	arg1	use					1557:1559	their use	1551:1559	their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis	1551:1744	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	8	79	theme	newborn	1358:1364	arg1	infants					1366:1372	newborn infants	1358:1372	newborn infants	1358:1372	The model predicts that individual differences in intracolonic oxygen in newborn infants can explain the observed individual variation in succession to anaerobic species, in particular Bifidobacterium species.					
36047700	12	80	theme	dominant	2064:2071	arg1	species					2099:2105	The frequently dominant anaerobic Bifidobacterium species	2049:2105	The frequently dominant anaerobic Bifidobacterium species benefit health	2049:2120	The frequently dominant anaerobic Bifidobacterium species benefit health, e.g., they can keep harmful competitors under control and modulate the intestinal immune response.					
36047700	6	81	theme	bacterial	997:1005	arg1	dynamics					1018:1025	spatial bacterial population dynamics	989:1025	spatial bacterial population dynamics	989:1025	To study if oxygen depletion suffices for the transition to Bifidobacterium species, here we introduced a multiscale mathematical model that considers metabolism, spatial bacterial population dynamics, and cross-feeding.					
36047700	0	82	theme	Infant	125:130	arg1	Microbiota					136:145	the Early Infant Gut Microbiota	115:145	the Early Infant Gut Microbiota	115:145	A Multiscale Spatiotemporal Model Including a Switch from Aerobic to Anaerobic Metabolism Reproduces Succession in the Early Infant Gut Microbiota.					
36047700	11	83	theme	infant	1931:1936	arg1	microbiota					1938:1947	the infant microbiota	1927:1947	the infant microbiota	1927:1947	IMPORTANCE The composition of the infant microbiota has a great impact on infant health, but its controlling factors are still incompletely understood.					
36047700	7	84	theme	AGORA	1104:1108	arg1	collection					1110:1119	the AGORA collection	1100:1119	the AGORA collection	1100:1119	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	13	85	theme	Controlling	2222:2232	arg1	factors					2234:2240	Controlling factors	2222:2240	Controlling factors	2222:2240	Controlling factors could include nutritional composition and intestinal mucus composition, as well as environmental factors, such as antibiotics.					
36047700	15	86	theme	Bifidobacterium	2645:2659	arg1	species					2661:2667	Bifidobacterium species	2645:2667	Bifidobacterium species	2645:2667	We present simulations that suggest that greater levels of intraintestinal oxygenation more strongly delay the dominance of Bifidobacterium species, explaining the observed variety of microbial composition and demonstrating the use of the model for hypothesis generation.					
36047700	9	87	from	concentrations	1684:1697	arg1	growth					1661:1666	fast growth	1656:1666	fast growth at high lactose concentrations	1656:1697	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	0	88	from	Succession	101:110	arg1	Microbiota					136:145	the Early Infant Gut Microbiota	115:145	the Early Infant Gut Microbiota	115:145	A Multiscale Spatiotemporal Model Including a Switch from Aerobic to Anaerobic Metabolism Reproduces Succession in the Early Infant Gut Microbiota.					
36047700	12	89	theme	immune	2205:2210	arg1	response					2212:2219	the intestinal immune response	2190:2219	the intestinal immune response	2190:2219	The frequently dominant anaerobic Bifidobacterium species benefit health, e.g., they can keep harmful competitors under control and modulate the intestinal immune response.					
36047700	7	90	theme	metabolic	1072:1080	arg1	data					1090:1093	publicly available metabolic network data	1053:1093	publicly available metabolic network data from the AGORA collection	1053:1119	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	10	91	from	succession	1865:1874	arg1	colon					1890:1894	the infant colon	1879:1894	the infant colon	1879:1894	The computational model thus allows us to test the internal plausibility of hypotheses for bacterial colonization and succession in the infant colon.					
36047700	5	92	theme	intracolonic	730:741	arg1	present					750:756	intracolonic oxygen present	730:756	intracolonic oxygen present in newborns	730:768	Succession to Bifidobacterium is thought to be due to consumption of intracolonic oxygen present in newborns by facultative anaerobes, including Enterobacteriaceae.					
36047700	8	93	theme	individual	1399:1408	arg1	variation					1410:1418	the observed individual variation	1386:1418	the observed individual variation in succession to anaerobic species	1386:1453	The model predicts that individual differences in intracolonic oxygen in newborn infants can explain the observed individual variation in succession to anaerobic species, in particular Bifidobacterium species.					
36047700	8	94	from	oxygen	1348:1353	arg1	infants					1366:1372	newborn infants	1358:1372	newborn infants	1358:1372	The model predicts that individual differences in intracolonic oxygen in newborn infants can explain the observed individual variation in succession to anaerobic species, in particular Bifidobacterium species.					
36047700	1	95	theme	human	152:156	arg1	microbiota					169:178	The human intestinal microbiota	148:178	The human intestinal microbiota	148:178	The human intestinal microbiota starts to form immediately after birth and is important for the health of the host.					
36047700	1	95	theme	human	152:156	arg1	important					226:234	important	226:234	important	226:234	The human intestinal microbiota starts to form immediately after birth and is important for the health of the host.					
36047700	5	96	theme	oxygen	743:748	arg1	present					750:756	intracolonic oxygen present	730:756	intracolonic oxygen present in newborns	730:768	Succession to Bifidobacterium is thought to be due to consumption of intracolonic oxygen present in newborns by facultative anaerobes, including Enterobacteriaceae.					
36047700	14	97	theme	ecology	2453:2459	arg1	framework					2393:2401	a modeling framework	2382:2401	a modeling framework of a metabolically realistic intestinal microbial ecology in which hypothetical scenarios can be tested and compared	2382:2518	We introduce a modeling framework of a metabolically realistic intestinal microbial ecology in which hypothetical scenarios can be tested and compared.					
36047700	7	98	theme	oxygen	1277:1282	arg1	influence					1252:1260	the influence	1248:1260	the influence of lactose and oxygen	1248:1282	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	15	99	theme	intraintestinal	2580:2594	arg1	oxygenation					2596:2606	intraintestinal oxygenation	2580:2606	intraintestinal oxygenation	2580:2606	We present simulations that suggest that greater levels of intraintestinal oxygenation more strongly delay the dominance of Bifidobacterium species, explaining the observed variety of microbial composition and demonstrating the use of the model for hypothesis generation.					
36047700	4	100	theme	Bifidobacterium	489:503	arg1	species					505:511	Bifidobacterium species	489:511	Bifidobacterium species	489:511	An early transition to Bifidobacterium species is associated with health benefits; for example, Bifidobacterium species repress growth of pathogenic competitors and modulate the immune response.					
36047700	14	101	theme	intestinal	2432:2441	arg1	ecology					2453:2459	a metabolically realistic intestinal microbial ecology	2406:2459	a metabolically realistic intestinal microbial ecology in which hypothetical scenarios can be tested and compared	2406:2518	We introduce a modeling framework of a metabolically realistic intestinal microbial ecology in which hypothetical scenarios can be tested and compared.					
36047700	2	102	theme	bacterial	311:319	arg1	species					321:327	facultatively anaerobic bacterial species	287:327	facultatively anaerobic bacterial species	287:327	During the first days, facultatively anaerobic bacterial species generally dominate, such as Enterobacteriaceae.					
36047700	5	103	theme	facultative	773:783	arg1	anaerobes					785:793	facultative anaerobes	773:793	facultative anaerobes	773:793	Succession to Bifidobacterium is thought to be due to consumption of intracolonic oxygen present in newborns by facultative anaerobes, including Enterobacteriaceae.					
36047700	5	103	theme	facultative	773:783	arg1	Enterobacteriaceae					806:823	Enterobacteriaceae	806:823	Enterobacteriaceae	806:823	Succession to Bifidobacterium is thought to be due to consumption of intracolonic oxygen present in newborns by facultative anaerobes, including Enterobacteriaceae.					
36047700	15	104	theme	microbial	2705:2713	arg1	composition					2715:2725	microbial composition	2705:2725	microbial composition	2705:2725	We present simulations that suggest that greater levels of intraintestinal oxygenation more strongly delay the dominance of Bifidobacterium species, explaining the observed variety of microbial composition and demonstrating the use of the model for hypothesis generation.					
36047700	9	105	theme	fast	1656:1659	arg1	growth					1661:1666	fast growth	1656:1666	fast growth at high lactose concentrations	1656:1697	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	10	106	theme	computational	1751:1763	arg1	model					1765:1769	The computational model	1747:1769	The computational model	1747:1769	The computational model thus allows us to test the internal plausibility of hypotheses for bacterial colonization and succession in the infant colon.					
36047700	0	107	theme	Multiscale	2:11	arg1	Model					28:32	A Multiscale Spatiotemporal Model	0:32	A Multiscale Spatiotemporal Model Including a Switch from Aerobic to Anaerobic Metabolism	0:88	A Multiscale Spatiotemporal Model Including a Switch from Aerobic to Anaerobic Metabolism Reproduces Succession in the Early Infant Gut Microbiota.					
36047700	11	108	from	impact	1961:1966	arg1	health					1978:1983	infant health	1971:1983	infant health	1971:1983	IMPORTANCE The composition of the infant microbiota has a great impact on infant health, but its controlling factors are still incompletely understood.					
36047700	11	109	theme	controlling	1994:2004	arg1	factors					2006:2012	its controlling factors	1990:2012	its controlling factors	1990:2012	IMPORTANCE The composition of the infant microbiota has a great impact on infant health, but its controlling factors are still incompletely understood.					
36047700	7	110	from	competition	1156:1166	arg1	environment					1230:1240	a gut-like environment	1219:1240	a gut-like environment	1219:1240	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	9	111	theme	lactose	1676:1682	arg1	concentrations					1684:1697	high lactose concentrations	1671:1697	high lactose concentrations	1671:1697	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	8	112	theme	Bifidobacterium	1470:1484	arg1	species					1486:1492	particular Bifidobacterium species	1459:1492	particular Bifidobacterium species	1459:1492	The model predicts that individual differences in intracolonic oxygen in newborn infants can explain the observed individual variation in succession to anaerobic species, in particular Bifidobacterium species.					
36047700	14	113	theme	modeling	2384:2391	arg1	framework					2393:2401	a modeling framework	2382:2401	a modeling framework of a metabolically realistic intestinal microbial ecology in which hypothetical scenarios can be tested and compared	2382:2518	We introduce a modeling framework of a metabolically realistic intestinal microbial ecology in which hypothetical scenarios can be tested and compared.					
36047700	7	114	theme	gut-like	1221:1228	arg1	environment					1230:1240	a gut-like environment	1219:1240	a gut-like environment	1219:1240	Using publicly available metabolic network data from the AGORA collection, the model simulates ab initio the competition of strictly and facultatively anaerobic species in a gut-like environment under the influence of lactose and oxygen.					
36047700	9	115	from	dominant	1526:1533	arg1	model					1542:1546	the model	1538:1546	the model	1538:1546	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	17	116	theme	oligosaccharide	2961:2975	arg1	modeling					2949:2956	detailed modeling	2940:2956	detailed modeling of oligosaccharide and mucin metabolism	2940:2996	Future versions will also include detailed modeling of oligosaccharide and mucin metabolism.					
36047700	17	117	theme	mucin	2981:2985	arg1	metabolism					2987:2996	mucin metabolism	2981:2996	mucin metabolism	2981:2996	Future versions will also include detailed modeling of oligosaccharide and mucin metabolism.					
36047700	10	118	theme	internal	1798:1805	arg1	plausibility					1807:1818	the internal plausibility	1794:1818	the internal plausibility of hypotheses for bacterial colonization and succession in the infant colon	1794:1894	The computational model thus allows us to test the internal plausibility of hypotheses for bacterial colonization and succession in the infant colon.					
36047700	6	119	theme	Bifidobacterium	886:900	arg1	species					902:908	Bifidobacterium species	886:908	Bifidobacterium species	886:908	To study if oxygen depletion suffices for the transition to Bifidobacterium species, here we introduced a multiscale mathematical model that considers metabolism, spatial bacterial population dynamics, and cross-feeding.					
36047700	16	120	theme	controlling	2839:2849	arg1	mixing					2881:2886	intestinal mixing	2870:2886	intestinal mixing	2870:2886	The framework allowed us to test a variety of controlling factors, including intestinal mixing and transit time.					
36047700	16	120	theme	controlling	2839:2849	arg1	time					2900:2903	transit time	2892:2903	transit time	2892:2903	The framework allowed us to test a variety of controlling factors, including intestinal mixing and transit time.					
36047700	16	120	theme	controlling	2839:2849	arg1	factors					2851:2857	controlling factors	2839:2857	controlling factors	2839:2857	The framework allowed us to test a variety of controlling factors, including intestinal mixing and transit time.					
36047700	8	121	from	differences	1320:1330	arg1	oxygen					1348:1353	intracolonic oxygen	1335:1353	intracolonic oxygen in newborn infants	1335:1372	The model predicts that individual differences in intracolonic oxygen in newborn infants can explain the observed individual variation in succession to anaerobic species, in particular Bifidobacterium species.					
36047700	11	122	theme	great	1955:1959	arg1	impact					1961:1966	a great impact	1953:1966	a great impact on infant health	1953:1983	IMPORTANCE The composition of the infant microbiota has a great impact on infant health, but its controlling factors are still incompletely understood.					
36047700	9	123	theme	Bifidobacterium	1495:1509	arg1	species					1511:1517	Bifidobacterium species	1495:1517	Bifidobacterium species	1495:1517	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	9	124	theme	balance	1729:1735	arg1	analysis					1737:1744	flux balance analysis	1724:1744	flux balance analysis	1724:1744	Bifidobacterium species became dominant in the model by their use of the bifid shunt, which allows Bifidobacterium to switch to suboptimal yield metabolism with fast growth at high lactose concentrations, as predicted here using flux balance analysis.					
36047700	13	125	theme	environmental	2325:2337	arg1	factors					2339:2345	environmental factors	2325:2345	environmental factors	2325:2345	Controlling factors could include nutritional composition and intestinal mucus composition, as well as environmental factors, such as antibiotics.					
36047700	13	125	theme	environmental	2325:2337	arg1	antibiotics					2356:2366	antibiotics	2356:2366	antibiotics	2356:2366	Controlling factors could include nutritional composition and intestinal mucus composition, as well as environmental factors, such as antibiotics.					
36558524	7	0	theme	reproduction	1454:1465	arg1	biology					1425:1431	the biology	1421:1431	the biology of Drosophila female reproduction	1421:1465	Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.					
36558524	7	1	theme	diet-induced	1483:1494	arg1	obesity					1496:1502	diet-induced obesity	1483:1502	diet-induced obesity	1483:1502	Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.					
36558524	2	2	from	duration	399:406	arg1	diet					379:382	Drosophila diet	368:382	Drosophila diet	368:382	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	3	from	function	285:292	arg1	diet					379:382	Drosophila diet	368:382	Drosophila diet	368:382	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	5	4	theme	immature	957:964	arg1	oocytes					966:972	immature oocytes	957:972	immature oocytes	957:972	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	5	5	theme	polycystic	1098:1107	arg1	PCOS					1125:1128	PCOS	1125:1128	PCOS	1125:1128	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	5	5	theme	polycystic	1098:1107	arg1	syndrome					1115:1122	polycystic ovary syndrome	1098:1122	polycystic ovary syndrome (PCOS)	1098:1129	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	7	6	theme	female	1447:1452	arg1	reproduction					1454:1465	Drosophila female reproduction	1436:1465	Drosophila female reproduction	1436:1465	Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.					
36558524	7	7	theme	disease	1549:1555	arg1	model					1557:1561	a potential disease model	1537:1561	a potential disease model for human ovarian dysfunction	1537:1591	Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.					
36558524	2	8	from	concentrations	301:314	arg1	diet					379:382	Drosophila diet	368:382	Drosophila diet	368:382	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	5	9	theme	condition	1085:1093	arg1	aspects					1064:1070	key aspects	1060:1070	key aspects of the human condition of polycystic ovary syndrome (PCOS)	1060:1129	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	5	10	theme	syndrome	1115:1122	arg1	condition					1085:1093	the human condition	1075:1093	the human condition of polycystic ovary syndrome (PCOS)	1075:1129	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	7	11	theme	type	1508:1511	arg1	diabetes					1516:1523	type II diabetes	1508:1523	type II diabetes	1508:1523	Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.					
36558524	4	12	theme	increased	706:714	arg1	fat					721:723	the physiological responses-including increased body fat	668:723	the physiological responses-including increased body fat	668:723	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	4	13	theme	treatment	793:801	arg1	condition					803:811	a treatment condition	791:811	a treatment condition identified by our modeling method	791:845	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	7	14	theme	human	1567:1571	arg1	dysfunction					1581:1591	human ovarian dysfunction	1567:1591	human ovarian dysfunction	1567:1591	Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.					
36558524	3	15	theme	physiological	555:567	arg1	they					518:521	they	518:521	they	518:521	Our results reveal complex interactions between sucrose and protein in impacting body fat and fertility when they are considered as an integrated physiological response.					
36558524	3	15	theme	physiological	555:567	arg1	response					569:576	an integrated physiological response	541:576	an integrated physiological response	541:576	Our results reveal complex interactions between sucrose and protein in impacting body fat and fertility when they are considered as an integrated physiological response.					
36558524	4	16	theme	modeling	621:628	arg1	approach					630:637	our quantitative modeling approach	604:637	our quantitative modeling approach	604:637	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	6	17	theme	ovarian	1281:1287	arg1	dysfunction					1289:1299	ovarian dysfunction	1281:1299	ovarian dysfunction	1281:1299	Our analysis of the dynamic progression of different aspects of diet-induced pathophysiology also suggests an order of the onset timing for obesity, ovarian dysfunction, and insulin resistance.					
36558524	1	18	theme	quantitative	74:85	arg1	studies					87:93	quantitative studies	74:93	quantitative studies	74:93	We perform quantitative studies to investigate the effect of high-calorie diet on Drosophila oogenesis.					
36558524	7	19	theme	ovarian	1573:1579	arg1	dysfunction					1581:1591	human ovarian dysfunction	1567:1591	human ovarian dysfunction	1567:1591	Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.					
36558524	1	20	theme	high-calorie	124:135	arg1	diet					137:140	high-calorie diet	124:140	high-calorie diet	124:140	We perform quantitative studies to investigate the effect of high-calorie diet on Drosophila oogenesis.					
36558524	4	21	theme	insulin	757:763	arg1	insensitivity-expected					765:786	ovarian insulin insensitivity-expected	749:786	ovarian insulin insensitivity-expected	749:786	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	2	22	theme	fertility	270:278	arg1	function					285:292	a function	283:292	a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration	283:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	22	theme	fertility	270:278	arg1	models					247:252	quadratic regression models	226:252	quadratic regression models of body fat and fertility	226:278	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	4	23	theme	responses-including	686:704	arg1	fat					721:723	the physiological responses-including increased body fat	668:723	the physiological responses-including increased body fat	668:723	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	4	24	theme	condition	803:811	arg1	fat					721:723	the physiological responses-including increased body fat	668:723	the physiological responses-including increased body fat	668:723	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	4	24	theme	condition	803:811	arg1	insensitivity-expected					765:786	ovarian insulin insensitivity-expected	749:786	ovarian insulin insensitivity-expected	749:786	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	4	24	theme	condition	803:811	arg1	fertility					734:742	reduced fertility	726:742	reduced fertility	726:742	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	3	25	theme	complex	428:434	arg1	interactions					436:447	complex interactions	428:447	complex interactions between sucrose and protein	428:475	Our results reveal complex interactions between sucrose and protein in impacting body fat and fertility when they are considered as an integrated physiological response.					
36558524	2	26	theme	concentrations	301:314	arg1	models					247:252	quadratic regression models	226:252	quadratic regression models of body fat and fertility	226:278	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	26	theme	concentrations	301:314	arg1	function					285:292	a function	283:292	a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration	283:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	27	theme	Drosophila	368:377	arg1	diet					379:382	Drosophila diet	368:382	Drosophila diet	368:382	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	6	28	theme	timing	1261:1266	arg1	order					1242:1246	an order	1239:1246	an order of the onset timing for obesity, ovarian dysfunction, and insulin resistance	1239:1323	Our analysis of the dynamic progression of different aspects of diet-induced pathophysiology also suggests an order of the onset timing for obesity, ovarian dysfunction, and insulin resistance.					
36558524	2	29	from	macronutrients	350:363	arg1	diet					379:382	Drosophila diet	368:382	Drosophila diet	368:382	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	4	30	theme	ovarian	749:755	arg1	insensitivity-expected					765:786	ovarian insulin insensitivity-expected	749:786	ovarian insulin insensitivity-expected	749:786	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	7	31	theme	quantitative	1367:1378	arg1	approaches					1389:1398	quantitative modeling approaches	1367:1398	quantitative modeling approaches	1367:1398	Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.					
36558524	4	32	theme	physiological	672:684	arg1	fat					721:723	the physiological responses-including increased body fat	668:723	the physiological responses-including increased body fat	668:723	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	7	33	theme	Drosophila	1436:1445	arg1	reproduction					1454:1465	Drosophila female reproduction	1436:1465	Drosophila female reproduction	1436:1465	Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.					
36558524	7	34	theme	approaches	1389:1398	arg1	utility					1356:1362	the utility	1352:1362	the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction	1352:1465	Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.					
36558524	6	35	theme	dynamic	1152:1158	arg1	progression					1160:1170	the dynamic progression	1148:1170	the dynamic progression of different aspects of diet-induced pathophysiology	1148:1223	Our analysis of the dynamic progression of different aspects of diet-induced pathophysiology also suggests an order of the onset timing for obesity, ovarian dysfunction, and insulin resistance.					
36558524	6	36	theme	onset	1255:1259	arg1	timing					1261:1266	the onset timing	1251:1266	the onset timing	1251:1266	Our analysis of the dynamic progression of different aspects of diet-induced pathophysiology also suggests an order of the onset timing for obesity, ovarian dysfunction, and insulin resistance.					
36558524	4	37	theme	approach	630:637	arg1	utility					593:599	the utility	589:599	the utility of our quantitative modeling approach	589:637	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	0	38	theme	Ovarian	28:34	arg1	Dysfunction					36:46	Ovarian Dysfunction	28:46	Ovarian Dysfunction in Drosophila	28:60	Modeling Obesity-Associated Ovarian Dysfunction in Drosophila.					
36558524	6	39	theme	diet-induced	1196:1207	arg1	pathophysiology					1209:1223	diet-induced pathophysiology	1196:1223	diet-induced pathophysiology	1196:1223	Our analysis of the dynamic progression of different aspects of diet-induced pathophysiology also suggests an order of the onset timing for obesity, ovarian dysfunction, and insulin resistance.					
36558524	5	40	theme	mature	1006:1011	arg1	oocytes					1013:1019	mature oocytes	1006:1019	mature oocytes	1006:1019	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	2	41	theme	design	196:201	arg1	method					209:214	the central composite design (CCD) method	174:214	the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration	174:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	3	42	theme	integrated	544:553	arg1	they					518:521	they	518:521	they	518:521	Our results reveal complex interactions between sucrose and protein in impacting body fat and fertility when they are considered as an integrated physiological response.					
36558524	3	42	theme	integrated	544:553	arg1	response					569:576	an integrated physiological response	541:576	an integrated physiological response	541:576	Our results reveal complex interactions between sucrose and protein in impacting body fat and fertility when they are considered as an integrated physiological response.					
36558524	2	43	theme	duration	399:406	arg1	models					247:252	quadratic regression models	226:252	quadratic regression models of body fat and fertility	226:278	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	43	theme	duration	399:406	arg1	function					285:292	a function	283:292	a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration	283:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	3	44	theme	body	490:493	arg1	fat					495:497	body fat	490:497	body fat	490:497	Our results reveal complex interactions between sucrose and protein in impacting body fat and fertility when they are considered as an integrated physiological response.					
36558524	2	45	theme	composite	186:194	arg1	method					209:214	the central composite design (CCD) method	174:214	the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration	174:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	46	theme	regression	236:245	arg1	function					285:292	a function	283:292	a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration	283:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	46	theme	regression	236:245	arg1	models					247:252	quadratic regression models	226:252	quadratic regression models of body fat and fertility	226:278	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	5	47	from	halt	980:983	arg1	production					992:1001	the production	988:1001	the production of mature oocytes	988:1019	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	2	48	theme	treatment	389:397	arg1	duration					399:406	treatment duration	389:406	treatment duration	389:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	4	49	theme	body	716:719	arg1	fat					721:723	the physiological responses-including increased body fat	668:723	the physiological responses-including increased body fat	668:723	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	5	50	theme	Drosophila	893:902	arg1	phenotype					1024:1032	a phenotype	1022:1032	a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS)	1022:1129	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	5	50	theme	Drosophila	893:902	arg1	phenotype					914:922	a Drosophila oogenesis phenotype	891:922	a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes	891:1019	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	5	51	theme	oogenesis	904:912	arg1	phenotype					1024:1032	a phenotype	1022:1032	a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS)	1022:1129	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	5	51	theme	oogenesis	904:912	arg1	phenotype					914:922	a Drosophila oogenesis phenotype	891:922	a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes	891:1019	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	2	52	theme	central	178:184	arg1	method					209:214	the central composite design (CCD) method	174:214	the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration	174:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	53	theme	quadratic	226:234	arg1	function					285:292	a function	283:292	a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration	283:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	53	theme	quadratic	226:234	arg1	models					247:252	quadratic regression models	226:252	quadratic regression models of body fat and fertility	226:278	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	6	54	theme	insulin	1306:1312	arg1	resistance					1314:1323	insulin resistance	1306:1323	insulin resistance	1306:1323	Our analysis of the dynamic progression of different aspects of diet-induced pathophysiology also suggests an order of the onset timing for obesity, ovarian dysfunction, and insulin resistance.					
36558524	5	55	theme	treatment	859:867	arg1	condition					869:877	this treatment condition	854:877	this treatment condition	854:877	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	5	56	theme	ovary	1109:1113	arg1	PCOS					1125:1128	PCOS	1125:1128	PCOS	1125:1128	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	5	56	theme	ovary	1109:1113	arg1	syndrome					1115:1122	polycystic ovary syndrome	1098:1122	polycystic ovary syndrome (PCOS)	1098:1129	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	7	57	theme	modeling	1380:1387	arg1	approaches					1389:1398	quantitative modeling approaches	1367:1398	quantitative modeling approaches	1367:1398	Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.					
36558524	5	58	from	accumulation	941:952	arg1	production					992:1001	the production	988:1001	the production of mature oocytes	988:1019	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	2	59	theme	sucrose	331:337	arg1	macronutrients					350:363	two major macronutrients	340:363	two major macronutrients in Drosophila diet	340:382	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	59	theme	sucrose	331:337	arg1	duration					399:406	treatment duration	389:406	treatment duration	389:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	59	theme	sucrose	331:337	arg1	concentrations					301:314	the concentrations	297:314	the concentrations of protein and sucrose	297:337	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	5	60	theme	key	1060:1062	arg1	aspects					1064:1070	key aspects	1060:1070	key aspects of the human condition of polycystic ovary syndrome (PCOS)	1060:1129	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	1	61	theme	diet	137:140	arg1	effect					114:119	the effect	110:119	the effect of high-calorie diet on Drosophila oogenesis	110:164	We perform quantitative studies to investigate the effect of high-calorie diet on Drosophila oogenesis.					
36558524	1	62	from	effect	114:119	arg1	oogenesis					156:164	Drosophila oogenesis	145:164	Drosophila oogenesis	145:164	We perform quantitative studies to investigate the effect of high-calorie diet on Drosophila oogenesis.					
36558524	6	63	theme	pathophysiology	1209:1223	arg1	aspects					1185:1191	different aspects	1175:1191	different aspects of diet-induced pathophysiology	1175:1223	Our analysis of the dynamic progression of different aspects of diet-induced pathophysiology also suggests an order of the onset timing for obesity, ovarian dysfunction, and insulin resistance.					
36558524	6	64	theme	aspects	1185:1191	arg1	progression					1160:1170	the dynamic progression	1148:1170	the dynamic progression of different aspects of diet-induced pathophysiology	1148:1223	Our analysis of the dynamic progression of different aspects of diet-induced pathophysiology also suggests an order of the onset timing for obesity, ovarian dysfunction, and insulin resistance.					
36558524	2	65	theme	fat	262:264	arg1	function					285:292	a function	283:292	a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration	283:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	65	theme	fat	262:264	arg1	models					247:252	quadratic regression models	226:252	quadratic regression models of body fat and fertility	226:278	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	66	from	diet	379:382	arg1	models					247:252	quadratic regression models	226:252	quadratic regression models of body fat and fertility	226:278	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	66	from	diet	379:382	arg1	function					285:292	a function	283:292	a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration	283:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	67	theme	protein	319:325	arg1	macronutrients					350:363	two major macronutrients	340:363	two major macronutrients in Drosophila diet	340:382	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	67	theme	protein	319:325	arg1	duration					399:406	treatment duration	389:406	treatment duration	389:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	67	theme	protein	319:325	arg1	concentrations					301:314	the concentrations	297:314	the concentrations of protein and sucrose	297:337	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	5	68	theme	oocytes	1013:1019	arg1	production					992:1001	the production	988:1001	the production of mature oocytes	988:1019	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	2	69	theme	CCD	204:206	arg1	method					209:214	the central composite design (CCD) method	174:214	the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration	174:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	70	theme	body	257:260	arg1	fat					262:264	body fat	257:264	body fat	257:264	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	1	71	theme	Drosophila	145:154	arg1	oogenesis					156:164	Drosophila oogenesis	145:164	Drosophila oogenesis	145:164	We perform quantitative studies to investigate the effect of high-calorie diet on Drosophila oogenesis.					
36558524	6	72	theme	progression	1160:1170	arg1	analysis					1136:1143	Our analysis	1132:1143	Our analysis of the dynamic progression of different aspects of diet-induced pathophysiology	1132:1223	Our analysis of the dynamic progression of different aspects of diet-induced pathophysiology also suggests an order of the onset timing for obesity, ovarian dysfunction, and insulin resistance.					
36558524	5	73	theme	oocytes	966:972	arg1	accumulation					941:952	an accumulation	938:952	an accumulation of immature oocytes	938:972	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	5	73	theme	oocytes	966:972	arg1	halt					980:983	a halt	978:983	a halt in the production of mature oocytes	978:1019	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	5	74	theme	human	1079:1083	arg1	condition					1085:1093	the human condition	1075:1093	the human condition of polycystic ovary syndrome (PCOS)	1075:1129	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	6	75	theme	different	1175:1183	arg1	aspects					1185:1191	different aspects	1175:1191	different aspects of diet-induced pathophysiology	1175:1223	Our analysis of the dynamic progression of different aspects of diet-induced pathophysiology also suggests an order of the onset timing for obesity, ovarian dysfunction, and insulin resistance.					
36558524	2	76	theme	macronutrients	350:363	arg1	models					247:252	quadratic regression models	226:252	quadratic regression models of body fat and fertility	226:278	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	2	76	theme	macronutrients	350:363	arg1	function					285:292	a function	283:292	a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration	283:406	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	5	77	contain	bears	1039:1043	arg1	phenotype					1024:1032	a phenotype	1022:1032	a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS)	1022:1129	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	5	77	contain	bears	1039:1043	arg2	resemblance					1045:1055	resemblance	1045:1055	resemblance	1045:1055	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	5	77	contain	bears	1039:1043	arg1	phenotype					914:922	a Drosophila oogenesis phenotype	891:922	a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes	891:1019	Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS).					
36558524	4	78	theme	quantitative	608:619	arg1	approach					630:637	our quantitative modeling approach	604:637	our quantitative modeling approach	604:637	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	0	79	from	Dysfunction	36:46	arg1	Drosophila					51:60	Drosophila	51:60	Drosophila	51:60	Modeling Obesity-Associated Ovarian Dysfunction in Drosophila.					
36558524	4	80	theme	modeling	831:838	arg1	method					840:845	our modeling method	827:845	our modeling method	827:845	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36558524	7	81	theme	potential	1539:1547	arg1	model					1557:1561	a potential disease model	1537:1561	a potential disease model for human ovarian dysfunction	1537:1591	Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.					
36558524	2	82	theme	major	344:348	arg1	macronutrients					350:363	two major macronutrients	340:363	two major macronutrients in Drosophila diet	340:382	We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration.					
36558524	4	83	theme	reduced	726:732	arg1	fertility					734:742	reduced fertility	726:742	reduced fertility	726:742	We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses-including increased body fat, reduced fertility, and ovarian insulin insensitivity-expected of a treatment condition identified by our modeling method.					
36593236	5	0	theme	hydroxyapatite	1110:1123	arg1	incorporation					1125:1137	hydroxyapatite incorporation	1110:1137	hydroxyapatite incorporation	1110:1137	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	4	1	theme	bilateral	839:847	arg1	ovariectomy					849:859	experimental bilateral ovariectomy	826:859	experimental bilateral ovariectomy	826:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	10	2	theme	bone	1882:1885	arg1	process					1894:1900	the bone repair process	1878:1900	the bone repair process	1878:1900	In conclusion, collagen/chitosan/hydroxyapatite scaffolds stimulate bone growth in vivo but abnormal conditions of bone fragility caused by gonadal hormone deficiency may have delayed the bone repair process.					
36593236	10	3	theme	bone	1809:1812	arg1	fragility					1814:1822	bone fragility	1809:1822	bone fragility caused by gonadal hormone deficiency	1809:1859	In conclusion, collagen/chitosan/hydroxyapatite scaffolds stimulate bone growth in vivo but abnormal conditions of bone fragility caused by gonadal hormone deficiency may have delayed the bone repair process.					
36593236	5	4	theme	collagen/chitosan/hydroxyapatite	1031:1062	arg1	scaffolds					1064:1072	collagen/chitosan/hydroxyapatite scaffolds	1031:1072	collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2)	1031:1178	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	4	5	theme	experimental	826:837	arg1	ovariectomy					849:859	experimental bilateral ovariectomy	826:859	experimental bilateral ovariectomy	826:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	6	6	theme	histomorphometric	1247:1263	arg1	analysis					1265:1272	histomorphometric analysis	1247:1272	histomorphometric analysis	1247:1272	The defect areas were submitted to macroscopic, radiological, and histomorphometric analysis.					
36593236	10	7	theme	abnormal	1786:1793	arg1	conditions					1795:1804	abnormal conditions	1786:1804	abnormal conditions of bone fragility caused by gonadal hormone deficiency	1786:1859	In conclusion, collagen/chitosan/hydroxyapatite scaffolds stimulate bone growth in vivo but abnormal conditions of bone fragility caused by gonadal hormone deficiency may have delayed the bone repair process.					
36593236	2	8	used	used	369:372	arg2	Collagen					331:338	Collagen	331:338	Collagen	331:338	Collagen and hydroxyapatite have been used for bone repair based on the concept of biomimetics, which can be combined with chitosan, forming a scaffold for cell adhesion and growth.					
36593236	2	8	used	used	369:372	arg2	hydroxyapatite					344:357	hydroxyapatite	344:357	hydroxyapatite	344:357	Collagen and hydroxyapatite have been used for bone repair based on the concept of biomimetics, which can be combined with chitosan, forming a scaffold for cell adhesion and growth.					
36593236	5	9	theme	O	939:939	arg1	groups					942:947	non-ovariectomized (NO) and ovariectomized (O) groups	895:947	groups	942:947	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	4	10	theme	Co	736:737	arg1	scaffolds					774:782	collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds	726:782	collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy	726:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	1	11	theme	bone	106:109	arg1	loss					111:114	bone loss	106:114	bone loss	106:114	Lesions with bone loss may require autologous grafts, which are considered the gold standard; however, natural or synthetic biomaterials are alternatives that can be used in clinical situations that require support for bone neoformation.					
36593236	8	12	theme	bone	1499:1502	arg1	defect					1504:1509	the bone defect	1495:1509	the bone defect	1495:1509	Bone formation starting from the margins of the bone defect were observed in all rats, with a greater volume in the NO groups, particularly the group receiving CoChHa2.					
36593236	10	13	theme	repair	1887:1892	arg1	process					1894:1900	the bone repair process	1878:1900	the bone repair process	1878:1900	In conclusion, collagen/chitosan/hydroxyapatite scaffolds stimulate bone growth in vivo but abnormal conditions of bone fragility caused by gonadal hormone deficiency may have delayed the bone repair process.					
36593236	4	14	theme	/chitosan	739:747	arg1	scaffolds					774:782	collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds	726:782	collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy	726:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	1	15	theme	clinical	267:274	arg1	situations					276:285	clinical situations	267:285	clinical situations that require support for bone neoformation	267:328	Lesions with bone loss may require autologous grafts, which are considered the gold standard; however, natural or synthetic biomaterials are alternatives that can be used in clinical situations that require support for bone neoformation.					
36593236	7	16	theme	biomaterials	1437:1448	arg1	biocompatibility					1413:1428	the biocompatibility	1409:1428	the biocompatibility of the biomaterials	1409:1448	No inflammatory processes were found in the tibial defect area that would indicate immune rejection of the scaffolds, thus confirming the biocompatibility of the biomaterials.					
36593236	4	17	theme	osteoregenerative	696:712	arg1	capacity					714:721	the osteoregenerative capacity	692:721	the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy	692:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	7	18	theme	immune	1358:1363	arg1	rejection					1365:1373	immune rejection	1358:1373	immune rejection of the scaffolds	1358:1390	No inflammatory processes were found in the tibial defect area that would indicate immune rejection of the scaffolds, thus confirming the biocompatibility of the biomaterials.					
36593236	5	19	dep	subgroups	969:977	arg1	control					980:986	control	980:986	control (empty defect)	980:1001	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	5	19	dep	subgroups	969:977	arg1	defect					995:1000	empty defect	989:1000	empty defect	989:1000	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	5	19	dep	subgroups	969:977	arg1	subgroups					1011:1019	two subgroups	1007:1019	two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2)	1007:1178	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	10	20	theme	hormone	1842:1848	arg1	deficiency					1850:1859	gonadal hormone deficiency	1834:1859	gonadal hormone deficiency	1834:1859	In conclusion, collagen/chitosan/hydroxyapatite scaffolds stimulate bone growth in vivo but abnormal conditions of bone fragility caused by gonadal hormone deficiency may have delayed the bone repair process.					
36593236	0	21	from	repair	62:67	arg1	rats					87:90	ovariectomized rats	72:90	ovariectomized rats	72:90	Collagen-chitosan-hydroxyapatite composite scaffolds for bone repair in ovariectomized rats.					
36593236	4	22	theme	hormone	797:803	arg1	deficiency					805:814	hormone deficiency	797:814	hormone deficiency caused by experimental bilateral ovariectomy	797:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	7	23	theme	inflammatory	1278:1289	arg1	processes					1291:1299	No inflammatory processes	1275:1299	No inflammatory processes	1275:1299	No inflammatory processes were found in the tibial defect area that would indicate immune rejection of the scaffolds, thus confirming the biocompatibility of the biomaterials.					
36593236	4	24	theme	Ch	750:751	arg1	scaffolds					774:782	collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds	726:782	collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy	726:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	8	25	theme	defect	1504:1509	arg1	margins					1484:1490	the margins	1480:1490	the margins of the bone defect	1480:1509	Bone formation starting from the margins of the bone defect were observed in all rats, with a greater volume in the NO groups, particularly the group receiving CoChHa2.					
36593236	10	26	theme	collagen/chitosan/hydroxyapatite	1709:1740	arg1	scaffolds					1742:1750	collagen/chitosan/hydroxyapatite scaffolds	1709:1750	collagen/chitosan/hydroxyapatite scaffolds	1709:1750	In conclusion, collagen/chitosan/hydroxyapatite scaffolds stimulate bone growth in vivo but abnormal conditions of bone fragility caused by gonadal hormone deficiency may have delayed the bone repair process.					
36593236	3	27	theme	hormone	553:559	arg1	deficiency					561:570	gonadal hormone deficiency	545:570	gonadal hormone deficiency	545:570	However, osteoporosis caused by gonadal hormone deficiency can thus compromise the expected results of the osseointegration of scaffolds.					
36593236	5	28	dep	CoChHa1	1149:1155	arg1	CoChHa2					1171:1177	CoChHa2	1171:1177	CoChHa2	1171:1177	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	5	28	dep	CoChHa1	1149:1155	arg1	situ					1143:1146	situ	1143:1146	situ	1143:1146	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	10	29	theme	bone	1762:1765	arg1	growth					1767:1772	bone growth	1762:1772	bone growth	1762:1772	In conclusion, collagen/chitosan/hydroxyapatite scaffolds stimulate bone growth in vivo but abnormal conditions of bone fragility caused by gonadal hormone deficiency may have delayed the bone repair process.					
36593236	0	30	theme	Collagen-chitosan-hydroxyapatite	0:31	arg1	composite					33:41	Collagen-chitosan-hydroxyapatite composite	0:41	Collagen-chitosan-hydroxyapatite composite	0:41	Collagen-chitosan-hydroxyapatite composite scaffolds for bone repair in ovariectomized rats.					
36593236	4	31	theme	collagen	726:733	arg1	scaffolds					774:782	collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds	726:782	collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy	726:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	5	32	theme	incorporation	1125:1137	arg1	methods					1099:1105	different methods	1089:1105	different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2)	1089:1178	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	9	33	located	found	1644:1648	arg2	formation					1630:1638	Less bone formation	1620:1638	Less bone formation	1620:1638	Less bone formation was found in the O subgroups when compared to the NO.					
36593236	9	33	located	found	1644:1648	arg1	subgroups					1659:1667	the O subgroups	1653:1667	the O subgroups	1653:1667	Less bone formation was found in the O subgroups when compared to the NO.					
36593236	7	34	theme	scaffolds	1382:1390	arg1	rejection					1365:1373	immune rejection	1358:1373	immune rejection of the scaffolds	1358:1390	No inflammatory processes were found in the tibial defect area that would indicate immune rejection of the scaffolds, thus confirming the biocompatibility of the biomaterials.					
36593236	7	35	theme	tibial	1319:1324	arg1	area					1333:1336	the tibial defect area	1315:1336	the tibial defect area that would indicate immune rejection of the scaffolds, thus confirming the biocompatibility of the biomaterials	1315:1448	No inflammatory processes were found in the tibial defect area that would indicate immune rejection of the scaffolds, thus confirming the biocompatibility of the biomaterials.					
36593236	5	36	theme	ovariectomized	923:936	arg1	groups					942:947	non-ovariectomized (NO) and ovariectomized (O) groups	895:947	groups	942:947	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	1	37	theme	natural	196:202	arg1	alternatives					234:245	alternatives	234:245	alternatives that can be used in clinical situations that require support for bone neoformation	234:328	Lesions with bone loss may require autologous grafts, which are considered the gold standard; however, natural or synthetic biomaterials are alternatives that can be used in clinical situations that require support for bone neoformation.					
36593236	1	37	theme	natural	196:202	arg1	biomaterials					217:228	natural or synthetic biomaterials	196:228	natural or synthetic biomaterials	196:228	Lesions with bone loss may require autologous grafts, which are considered the gold standard; however, natural or synthetic biomaterials are alternatives that can be used in clinical situations that require support for bone neoformation.					
36593236	5	38	dep	divided	882:888	arg1	divided					950:956	divided	950:956	divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2)	950:1178	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	5	39	theme	ex	1162:1163	arg1	incorporation					1125:1137	hydroxyapatite incorporation	1110:1137	hydroxyapatite incorporation	1110:1137	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	3	40	theme	scaffolds	640:648	arg1	osseointegration					620:635	the osseointegration	616:635	the osseointegration of scaffolds	616:648	However, osteoporosis caused by gonadal hormone deficiency can thus compromise the expected results of the osseointegration of scaffolds.					
36593236	3	41	theme	gonadal	545:551	arg1	deficiency					561:570	gonadal hormone deficiency	545:570	gonadal hormone deficiency	545:570	However, osteoporosis caused by gonadal hormone deficiency can thus compromise the expected results of the osseointegration of scaffolds.					
36593236	2	42	theme	biomimetics	414:424	arg1	concept					403:409	the concept	399:409	the concept of biomimetics, which can be combined with chitosan	399:461	Collagen and hydroxyapatite have been used for bone repair based on the concept of biomimetics, which can be combined with chitosan, forming a scaffold for cell adhesion and growth.					
36593236	1	43	with	Lesions	93:99	arg1	loss					111:114	bone loss	106:114	bone loss	106:114	Lesions with bone loss may require autologous grafts, which are considered the gold standard; however, natural or synthetic biomaterials are alternatives that can be used in clinical situations that require support for bone neoformation.					
36593236	5	44	theme	non-ovariectomized	895:912	arg1	NO					915:916	non-ovariectomized (NO) and ovariectomized (O) groups	895:947	NO	915:916	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	10	45	theme	fragility	1814:1822	arg1	conditions					1795:1804	abnormal conditions	1786:1804	abnormal conditions of bone fragility caused by gonadal hormone deficiency	1786:1859	In conclusion, collagen/chitosan/hydroxyapatite scaffolds stimulate bone growth in vivo but abnormal conditions of bone fragility caused by gonadal hormone deficiency may have delayed the bone repair process.					
36593236	1	46	theme	synthetic	207:215	arg1	alternatives					234:245	alternatives	234:245	alternatives that can be used in clinical situations that require support for bone neoformation	234:328	Lesions with bone loss may require autologous grafts, which are considered the gold standard; however, natural or synthetic biomaterials are alternatives that can be used in clinical situations that require support for bone neoformation.					
36593236	1	46	theme	synthetic	207:215	arg1	biomaterials					217:228	natural or synthetic biomaterials	196:228	natural or synthetic biomaterials	196:228	Lesions with bone loss may require autologous grafts, which are considered the gold standard; however, natural or synthetic biomaterials are alternatives that can be used in clinical situations that require support for bone neoformation.					
36593236	0	47	theme	bone	57:60	arg1	repair					62:67	bone repair	57:67	bone repair in ovariectomized rats	57:90	Collagen-chitosan-hydroxyapatite composite scaffolds for bone repair in ovariectomized rats.					
36593236	5	48	theme	in	1140:1141	arg1	CoChHa1					1149:1155	CoChHa1	1149:1155	CoChHa1	1149:1155	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	8	49	from	volume	1553:1558	arg1	group					1595:1599	the group	1591:1599	the group receiving CoChHa2	1591:1617	Bone formation starting from the margins of the bone defect were observed in all rats, with a greater volume in the NO groups, particularly the group receiving CoChHa2.					
36593236	8	49	from	volume	1553:1558	arg1	groups					1570:1575	the NO groups	1563:1575	the NO groups	1563:1575	Bone formation starting from the margins of the bone defect were observed in all rats, with a greater volume in the NO groups, particularly the group receiving CoChHa2.					
36593236	3	50	theme	osseointegration	620:635	arg1	results					605:611	the expected results	592:611	the expected results of the osseointegration of scaffolds	592:648	However, osteoporosis caused by gonadal hormone deficiency can thus compromise the expected results of the osseointegration of scaffolds.					
36593236	4	51	theme	scaffolds	774:782	arg1	capacity					714:721	the osteoregenerative capacity	692:721	the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy	692:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	8	52	located	observed	1516:1523	arg1	rats					1532:1535	all rats	1528:1535	all rats	1528:1535	Bone formation starting from the margins of the bone defect were observed in all rats, with a greater volume in the NO groups, particularly the group receiving CoChHa2.					
36593236	8	52	located	observed	1516:1523	arg2	formation					1456:1464	Bone formation	1451:1464	Bone formation starting from the margins of the bone defect	1451:1509	Bone formation starting from the margins of the bone defect were observed in all rats, with a greater volume in the NO groups, particularly the group receiving CoChHa2.					
36593236	0	53	theme	ovariectomized	72:85	arg1	rats					87:90	ovariectomized rats	72:90	ovariectomized rats	72:90	Collagen-chitosan-hydroxyapatite composite scaffolds for bone repair in ovariectomized rats.					
36593236	1	54	theme	bone	312:315	arg1	neoformation					317:328	bone neoformation	312:328	bone neoformation	312:328	Lesions with bone loss may require autologous grafts, which are considered the gold standard; however, natural or synthetic biomaterials are alternatives that can be used in clinical situations that require support for bone neoformation.					
36593236	6	55	theme	defect	1185:1190	arg1	areas					1192:1196	The defect areas	1181:1196	The defect areas	1181:1196	The defect areas were submitted to macroscopic, radiological, and histomorphometric analysis.					
36593236	4	56	from	rats	787:790	arg1	capacity					714:721	the osteoregenerative capacity	692:721	the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy	692:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	1	57	theme	autologous	128:137	arg1	grafts					139:144	autologous grafts	128:144	autologous grafts	128:144	Lesions with bone loss may require autologous grafts, which are considered the gold standard; however, natural or synthetic biomaterials are alternatives that can be used in clinical situations that require support for bone neoformation.					
36593236	9	58	theme	O	1657:1657	arg1	subgroups					1659:1667	the O subgroups	1653:1667	the O subgroups	1653:1667	Less bone formation was found in the O subgroups when compared to the NO.					
36593236	8	59	theme	greater	1545:1551	arg1	volume					1553:1558	a greater volume	1543:1558	a greater volume in the NO groups, particularly the group receiving CoChHa2	1543:1617	Bone formation starting from the margins of the bone defect were observed in all rats, with a greater volume in the NO groups, particularly the group receiving CoChHa2.					
36593236	9	60	theme	bone	1625:1628	arg1	formation					1630:1638	Less bone formation	1620:1638	Less bone formation	1620:1638	Less bone formation was found in the O subgroups when compared to the NO.					
36593236	8	61	theme	Bone	1451:1454	arg1	formation					1456:1464	Bone formation	1451:1464	Bone formation starting from the margins of the bone defect	1451:1509	Bone formation starting from the margins of the bone defect were observed in all rats, with a greater volume in the NO groups, particularly the group receiving CoChHa2.					
36593236	5	62	theme	empty	989:993	arg1	control					980:986	control	980:986	control (empty defect)	980:1001	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	5	62	theme	empty	989:993	arg1	defect					995:1000	empty defect	989:1000	empty defect	989:1000	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	1	63	used	used	259:262	arg2	biomaterials					217:228	natural or synthetic biomaterials	196:228	natural or synthetic biomaterials	196:228	Lesions with bone loss may require autologous grafts, which are considered the gold standard; however, natural or synthetic biomaterials are alternatives that can be used in clinical situations that require support for bone neoformation.					
36593236	1	63	used	used	259:262	arg2	alternatives					234:245	alternatives	234:245	alternatives that can be used in clinical situations that require support for bone neoformation	234:328	Lesions with bone loss may require autologous grafts, which are considered the gold standard; however, natural or synthetic biomaterials are alternatives that can be used in clinical situations that require support for bone neoformation.					
36593236	5	64	theme	CoChHa1	1149:1155	arg1	incorporation					1125:1137	hydroxyapatite incorporation	1110:1137	hydroxyapatite incorporation	1110:1137	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	7	65	located	found	1306:1310	arg1	area					1333:1336	the tibial defect area	1315:1336	the tibial defect area that would indicate immune rejection of the scaffolds, thus confirming the biocompatibility of the biomaterials	1315:1448	No inflammatory processes were found in the tibial defect area that would indicate immune rejection of the scaffolds, thus confirming the biocompatibility of the biomaterials.					
36593236	7	65	located	found	1306:1310	arg2	processes					1291:1299	No inflammatory processes	1275:1299	No inflammatory processes	1275:1299	No inflammatory processes were found in the tibial defect area that would indicate immune rejection of the scaffolds, thus confirming the biocompatibility of the biomaterials.					
36593236	7	66	theme	defect	1326:1331	arg1	area					1333:1336	the tibial defect area	1315:1336	the tibial defect area that would indicate immune rejection of the scaffolds, thus confirming the biocompatibility of the biomaterials	1315:1448	No inflammatory processes were found in the tibial defect area that would indicate immune rejection of the scaffolds, thus confirming the biocompatibility of the biomaterials.					
36593236	4	67	from	scaffolds	774:782	arg1	rats					787:790	rats	787:790	rats with hormone deficiency caused by experimental bilateral ovariectomy	787:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	4	68	from	capacity	714:721	arg1	rats					787:790	rats	787:790	rats with hormone deficiency caused by experimental bilateral ovariectomy	787:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	10	69	theme	gonadal	1834:1840	arg1	deficiency					1850:1859	gonadal hormone deficiency	1834:1859	gonadal hormone deficiency	1834:1859	In conclusion, collagen/chitosan/hydroxyapatite scaffolds stimulate bone growth in vivo but abnormal conditions of bone fragility caused by gonadal hormone deficiency may have delayed the bone repair process.					
36593236	4	70	theme	/hydroxyapatite	753:767	arg1	scaffolds					774:782	collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds	726:782	collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy	726:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	4	71	with	rats	787:790	arg1	deficiency					805:814	hormone deficiency	797:814	hormone deficiency caused by experimental bilateral ovariectomy	797:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	2	72	theme	cell	487:490	arg1	adhesion					492:499	cell adhesion	487:499	cell adhesion	487:499	Collagen and hydroxyapatite have been used for bone repair based on the concept of biomimetics, which can be combined with chitosan, forming a scaffold for cell adhesion and growth.					
36593236	3	73	theme	expected	596:603	arg1	results					605:611	the expected results	592:611	the expected results of the osseointegration of scaffolds	592:648	However, osteoporosis caused by gonadal hormone deficiency can thus compromise the expected results of the osseointegration of scaffolds.					
36593236	2	74	theme	bone	378:381	arg1	repair					383:388	bone repair	378:388	bone repair based on the concept of biomimetics, which can be combined with chitosan	378:461	Collagen and hydroxyapatite have been used for bone repair based on the concept of biomimetics, which can be combined with chitosan, forming a scaffold for cell adhesion and growth.					
36593236	8	75	theme	NO	1567:1568	arg1	group					1595:1599	the group	1591:1599	the group receiving CoChHa2	1591:1617	Bone formation starting from the margins of the bone defect were observed in all rats, with a greater volume in the NO groups, particularly the group receiving CoChHa2.					
36593236	8	75	theme	NO	1567:1568	arg1	groups					1570:1575	the NO groups	1563:1575	the NO groups	1563:1575	Bone formation starting from the margins of the bone defect were observed in all rats, with a greater volume in the NO groups, particularly the group receiving CoChHa2.					
36593236	5	76	dep	ex	1162:1163	arg1	situ					1165:1168	situ	1165:1168	situ	1165:1168	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	4	77	theme	Ha	770:771	arg1	scaffolds					774:782	collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds	726:782	collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy	726:859	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
36593236	5	78	theme	different	1089:1097	arg1	methods					1099:1105	different methods	1089:1105	different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2)	1089:1178	Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2).					
36593236	4	79	theme	study	667:671	arg1	aim					655:657	The aim	651:657	The aim of this study	651:671	The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy.					
35108450	1	0	from	effects	276:282	arg1	balance					294:300	energy balance	287:300	energy balance	287:300	SCOPE Dietary protein, prebiotic fiber, and exercise individually have been shown to aid in weight loss; however less is known of their combined effects on energy balance.					
35108450	1	1	theme	weight	223:228	arg1	loss					230:233	weight loss	223:233	weight loss	223:233	SCOPE Dietary protein, prebiotic fiber, and exercise individually have been shown to aid in weight loss; however less is known of their combined effects on energy balance.					
35108450	8	2	theme	modulated	1764:1772	arg1	microbiota					1778:1787	selectively modulated gut microbiota and plasma metabolites	1752:1810	microbiota	1778:1787	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	8	3	theme	CONCLUSION	1592:1601	arg1	Combination					1603:1613	CONCLUSION Combination	1592:1613	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise	1592:1669	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	3	4	theme	Obese	452:456	arg1	rats					463:466	Obese male rats	452:466	Obese male rats	452:466	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	1	5	theme	prebiotic	154:162	arg1	fiber					164:168	prebiotic fiber	154:168	prebiotic fiber	154:168	SCOPE Dietary protein, prebiotic fiber, and exercise individually have been shown to aid in weight loss; however less is known of their combined effects on energy balance.					
35108450	1	5	theme	prebiotic	154:162	arg1	SCOPE					131:135	SCOPE Dietary protein, prebiotic fiber, and exercise	131:182	SCOPE Dietary protein, prebiotic fiber, and exercise	131:182	SCOPE Dietary protein, prebiotic fiber, and exercise individually have been shown to aid in weight loss; however less is known of their combined effects on energy balance.					
35108450	4	6	theme	respiratory	739:749	arg1	quotient					751:758	respiratory quotient	739:758	respiratory quotient	739:758	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	6	7	theme	whey-inulin	1096:1106	arg1	diets					1108:1112	Egg-cellulose, egg-inulin, and whey-inulin diets	1065:1112	Egg-cellulose, egg-inulin, and whey-inulin diets	1065:1112	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	8	8	theme	egg	1626:1628	arg1	Combination					1603:1613	CONCLUSION Combination	1592:1613	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise	1592:1669	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	7	9	theme	butyric	1526:1532	arg1	acid					1534:1537	plasma butyric acid	1519:1537	plasma butyric acid the most strongly associated to metagenome function	1519:1589	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	5	10	theme	CCK1-receptor	908:920	arg1	blockade					922:929	CCK1-receptor blockade	908:929	CCK1-receptor blockade	908:929	Notably, CCK1-receptor blockade attenuated the hypophagic effects of diets and in particular whey-inulin diet, and β-adrenergic blockade reduced EE across all diets.					
35108450	8	11	theme	whey	1633:1636	arg1	protein					1638:1644	whey protein	1633:1644	whey protein	1633:1644	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	3	12	theme	treadmill	655:663	arg1	exercise					665:672	treadmill exercise	655:672	treadmill exercise	655:672	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	0	13	theme	Obese	119:123	arg1	Rats					125:128	Exercising Obese Rats	108:128	Exercising Obese Rats	108:128	Diets Containing Egg or Whey Protein and Inulin Fiber Improve Energy Balance and Modulate Gut Microbiota in Exercising Obese Rats.					
35108450	4	14	theme	increased	766:774	arg1	EE					796:797	EE	796:797	EE	796:797	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	4	14	theme	increased	766:774	arg1	expenditure					783:793	increased energy expenditure	766:793	increased energy expenditure (EE)	766:798	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	6	15	theme	egg-inulin	1080:1089	arg1	diets					1108:1112	Egg-cellulose, egg-inulin, and whey-inulin diets	1065:1112	Egg-cellulose, egg-inulin, and whey-inulin diets	1065:1112	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	3	16	theme	high	497:500	arg1	protein					502:508	high protein and fiber contents	497:527	protein	502:508	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	7	17	theme	metabolites	1476:1486	arg1	subset					1425:1430	a subset	1423:1430	a subset of predicted metagenome pathways and plasma metabolites	1423:1486	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	6	18	theme	Egg-cellulose	1065:1077	arg1	diets					1108:1112	Egg-cellulose, egg-inulin, and whey-inulin diets	1065:1112	Egg-cellulose, egg-inulin, and whey-inulin diets	1065:1112	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	3	19	theme	egg	534:536	arg1	protein					538:544	egg protein	534:544	egg protein	534:544	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	6	20	theme	hepatic	1247:1253	arg1	metabolism					1263:1272	upregulated hepatic glucose metabolism	1235:1272	upregulated hepatic glucose metabolism	1235:1272	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	6	21	theme	decreased	1291:1299	arg1	insulin					1308:1314	decreased plasma insulin	1291:1314	decreased plasma insulin	1291:1314	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	7	22	theme	metagenome	1445:1454	arg1	pathways					1456:1463	predicted metagenome pathways	1435:1463	predicted metagenome pathways	1435:1463	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	0	23	theme	Energy	62:67	arg1	Balance					69:75	Energy Balance	62:75	Energy Balance	62:75	Diets Containing Egg or Whey Protein and Inulin Fiber Improve Energy Balance and Modulate Gut Microbiota in Exercising Obese Rats.					
35108450	5	24	theme	particular	981:990	arg1	diet					1004:1007	particular whey-inulin diet	981:1007	particular whey-inulin diet	981:1007	Notably, CCK1-receptor blockade attenuated the hypophagic effects of diets and in particular whey-inulin diet, and β-adrenergic blockade reduced EE across all diets.					
35108450	8	25	theme	glucose	1696:1702	arg1	metabolism					1704:1713	glucose metabolism	1696:1713	glucose metabolism	1696:1713	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	5	26	from	effects	957:963	arg1	diet					1004:1007	particular whey-inulin diet	981:1007	particular whey-inulin diet	981:1007	Notably, CCK1-receptor blockade attenuated the hypophagic effects of diets and in particular whey-inulin diet, and β-adrenergic blockade reduced EE across all diets.					
35108450	4	27	theme	upregulated	805:815	arg1	transcripts					817:827	upregulated transcripts	805:827	upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut	805:896	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	5	28	theme	hypophagic	946:955	arg1	effects					957:963	the hypophagic effects	942:963	the hypophagic effects of diets and in particular whey-inulin diet	942:1007	Notably, CCK1-receptor blockade attenuated the hypophagic effects of diets and in particular whey-inulin diet, and β-adrenergic blockade reduced EE across all diets.					
35108450	7	29	theme	metagenome	1571:1580	arg1	function					1582:1589	metagenome function	1571:1589	metagenome function	1571:1589	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	3	30	from	protein	621:627	arg1	protein					502:508	high protein and fiber contents	497:527	protein	502:508	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	3	30	from	protein	621:627	arg1	contents					520:527	high protein and fiber contents	497:527	contents	520:527	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	0	31	theme	Gut	90:92	arg1	Microbiota					94:103	Gut Microbiota	90:103	Gut Microbiota in Exercising Obese Rats	90:128	Diets Containing Egg or Whey Protein and Inulin Fiber Improve Energy Balance and Modulate Gut Microbiota in Exercising Obese Rats.					
35108450	8	32	theme	anorectic	1728:1736	arg1	hormones					1738:1745	upregulated anorectic hormones	1716:1745	upregulated anorectic hormones	1716:1745	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	4	33	theme	distal	887:892	arg1	gut					894:896	distal gut	887:896	distal gut	887:896	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	3	34	with	diets	486:490	arg1	protein					502:508	high protein and fiber contents	497:527	protein	502:508	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	3	34	with	diets	486:490	arg1	contents					520:527	high protein and fiber contents	497:527	contents	520:527	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	4	35	from	proglucagon	872:882	arg1	gut					894:896	distal gut	887:896	distal gut	887:896	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	4	36	theme	peptide	856:862	arg1	YY					864:865	peptide YY	856:865	peptide YY	856:865	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	4	37	theme	enriched	696:703	arg1	diets					705:709	inulin enriched diets	689:709	inulin enriched diets	689:709	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	0	38	theme	Whey	24:27	arg1	Protein					29:35	Whey Protein	24:35	Whey Protein	24:35	Diets Containing Egg or Whey Protein and Inulin Fiber Improve Energy Balance and Modulate Gut Microbiota in Exercising Obese Rats.					
35108450	1	39	theme	combined	267:274	arg1	effects					276:282	their combined effects	261:282	their combined effects on energy balance	261:300	SCOPE Dietary protein, prebiotic fiber, and exercise individually have been shown to aid in weight loss; however less is known of their combined effects on energy balance.					
35108450	7	40	theme	microbial	1372:1380	arg1	composition					1382:1392	altered gut microbial composition	1360:1392	altered gut microbial composition	1360:1392	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	2	41	with	diets	318:322	arg1	exercise					356:363	exercise	356:363	exercise	356:363	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota, were determined.					
35108450	2	42	theme	diets	318:322	arg1	effects					307:313	The effects	303:313	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota,	303:413	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota, were determined.					
35108450	6	43	dep	decreased	1114:1122	arg1	leptin					1320:1325	leptin	1320:1325	leptin	1320:1325	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	6	43	dep	decreased	1114:1122	arg1	insulin					1308:1314	decreased plasma insulin	1291:1314	decreased plasma insulin	1291:1314	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	3	44	from	protein	538:544	arg1	protein					502:508	high protein and fiber contents	497:527	protein	502:508	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	3	44	from	protein	538:544	arg1	contents					520:527	high protein and fiber contents	497:527	contents	520:527	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	3	45	theme	whey	585:588	arg1	protein					590:596	whey protein	585:596	whey protein	585:596	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	8	46	theme	gut	1774:1776	arg1	microbiota					1778:1787	selectively modulated gut microbiota and plasma metabolites	1752:1810	microbiota	1778:1787	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	3	47	theme	male	458:461	arg1	rats					463:466	Obese male rats	452:466	Obese male rats	452:466	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	3	48	from	cellulose	550:558	arg1	protein					502:508	high protein and fiber contents	497:527	protein	502:508	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	3	48	from	cellulose	550:558	arg1	contents					520:527	high protein and fiber contents	497:527	contents	520:527	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	3	49	theme	whey	616:619	arg1	protein					621:627	whey protein	616:627	whey protein	616:627	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	6	50	theme	weight	1124:1129	arg1	gain					1131:1134	weight gain	1124:1134	weight gain	1124:1134	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	4	51	theme	energy	721:726	arg1	RQ					761:762	RQ	761:762	RQ	761:762	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	4	51	theme	energy	721:726	arg1	intake					728:733	energy intake	721:733	energy intake	721:733	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	2	52	from	effects	307:313	arg1	hormones					385:392	hormones	385:392	hormones	385:392	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota, were determined.					
35108450	2	52	from	effects	307:313	arg1	balance					376:382	energy balance	369:382	energy balance	369:382	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota, were determined.					
35108450	2	52	from	effects	307:313	arg1	microbiota					403:412	gut microbiota	399:412	gut microbiota	399:412	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota, were determined.					
35108450	3	53	theme	high-fat	477:484	arg1	diets					486:490	high-fat diets	477:490	high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin	477:638	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	7	54	theme	plasma	1519:1524	arg1	acid					1534:1537	plasma butyric acid	1519:1537	plasma butyric acid the most strongly associated to metagenome function	1519:1589	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	8	55	theme	plasma	1793:1798	arg1	metabolites					1800:1810	selectively modulated gut microbiota and plasma metabolites	1752:1810	metabolites	1800:1810	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	8	56	with	Combination	1603:1613	arg1	inulin					1651:1656	inulin	1651:1656	inulin	1651:1656	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	8	56	with	Combination	1603:1613	arg1	exercise					1662:1669	exercise	1662:1669	exercise	1662:1669	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	0	57	theme	Exercising	108:117	arg1	Rats					125:128	Exercising Obese Rats	108:128	Exercising Obese Rats	108:128	Diets Containing Egg or Whey Protein and Inulin Fiber Improve Energy Balance and Modulate Gut Microbiota in Exercising Obese Rats.					
35108450	8	58	theme	dietary	1618:1624	arg1	egg					1626:1628	dietary egg	1618:1628	dietary egg	1618:1628	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	6	59	theme	lipogenic	1181:1189	arg1	transcripts					1191:1201	decreased lipogenic transcripts	1171:1201	decreased lipogenic transcripts	1171:1201	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	3	60	from	protein	590:596	arg1	protein					502:508	high protein and fiber contents	497:527	protein	502:508	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	3	60	from	protein	590:596	arg1	contents					520:527	high protein and fiber contents	497:527	contents	520:527	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	8	61	theme	protein	1638:1644	arg1	Combination					1603:1613	CONCLUSION Combination	1592:1613	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise	1592:1669	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	6	62	dep	transcripts	1191:1201	arg1	transcripts					1274:1284	transcripts	1274:1284	transcripts	1274:1284	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	7	63	theme	plasma	1469:1474	arg1	metabolites					1476:1486	plasma metabolites	1469:1486	plasma metabolites	1469:1486	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	0	64	from	Microbiota	94:103	arg1	Rats					125:128	Exercising Obese Rats	108:128	Exercising Obese Rats	108:128	Diets Containing Egg or Whey Protein and Inulin Fiber Improve Energy Balance and Modulate Gut Microbiota in Exercising Obese Rats.					
35108450	6	65	theme	hepatic	1152:1158	arg1	lipidosis					1160:1168	hepatic lipidosis	1152:1168	hepatic lipidosis	1152:1168	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	7	66	theme	pathways	1456:1463	arg1	subset					1425:1430	a subset	1423:1430	a subset of predicted metagenome pathways and plasma metabolites	1423:1486	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	4	67	theme	energy	776:781	arg1	EE					796:797	EE	796:797	EE	796:797	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	4	67	theme	energy	776:781	arg1	expenditure					783:793	increased energy expenditure	766:793	increased energy expenditure (EE)	766:798	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	6	68	theme	glucose	1255:1261	arg1	metabolism					1263:1272	upregulated hepatic glucose metabolism	1235:1272	upregulated hepatic glucose metabolism	1235:1272	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	3	69	theme	fiber	514:518	arg1	contents					520:527	high protein and fiber contents	497:527	contents	520:527	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	7	70	theme	predicted	1435:1443	arg1	pathways					1456:1463	predicted metagenome pathways	1435:1463	predicted metagenome pathways	1435:1463	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	6	71	theme	upregulated	1235:1245	arg1	metabolism					1263:1272	upregulated hepatic glucose metabolism	1235:1272	upregulated hepatic glucose metabolism	1235:1272	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	1	72	theme	energy	287:292	arg1	balance					294:300	energy balance	287:300	energy balance	287:300	SCOPE Dietary protein, prebiotic fiber, and exercise individually have been shown to aid in weight loss; however less is known of their combined effects on energy balance.					
35108450	8	73	theme	energy	1680:1685	arg1	balance					1687:1693	energy balance	1680:1693	energy balance	1680:1693	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	1	74	theme	Dietary	137:143	arg1	SCOPE					131:135	SCOPE Dietary protein, prebiotic fiber, and exercise	131:182	SCOPE Dietary protein, prebiotic fiber, and exercise	131:182	SCOPE Dietary protein, prebiotic fiber, and exercise individually have been shown to aid in weight loss; however less is known of their combined effects on energy balance.					
35108450	1	74	theme	Dietary	137:143	arg1	protein					145:151	Dietary protein	137:151	Dietary protein	137:151	SCOPE Dietary protein, prebiotic fiber, and exercise individually have been shown to aid in weight loss; however less is known of their combined effects on energy balance.					
35108450	2	75	theme	gut	399:401	arg1	microbiota					403:412	gut microbiota	399:412	gut microbiota	399:412	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota, were determined.					
35108450	7	76	theme	altered	1360:1366	arg1	composition					1382:1392	altered gut microbial composition	1360:1392	altered gut microbial composition	1360:1392	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	5	77	theme	whey-inulin	992:1002	arg1	diet					1004:1007	particular whey-inulin diet	981:1007	particular whey-inulin diet	981:1007	Notably, CCK1-receptor blockade attenuated the hypophagic effects of diets and in particular whey-inulin diet, and β-adrenergic blockade reduced EE across all diets.					
35108450	6	78	theme	glycemic	1213:1220	arg1	control					1222:1228	improved glycemic control	1204:1228	improved glycemic control	1204:1228	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	5	79	theme	diets	968:972	arg1	effects					957:963	the hypophagic effects	942:963	the hypophagic effects of diets and in particular whey-inulin diet	942:1007	Notably, CCK1-receptor blockade attenuated the hypophagic effects of diets and in particular whey-inulin diet, and β-adrenergic blockade reduced EE across all diets.					
35108450	8	80	theme	upregulated	1716:1726	arg1	hormones					1738:1745	upregulated anorectic hormones	1716:1745	upregulated anorectic hormones	1716:1745	CONCLUSION Combination of dietary egg or whey protein with inulin and exercise improved energy balance, glucose metabolism, upregulated anorectic hormones, and selectively modulated gut microbiota and plasma metabolites.					
35108450	2	81	theme	energy	369:374	arg1	balance					376:382	energy balance	369:382	energy balance	369:382	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota, were determined.					
35108450	2	82	theme	high	324:327	arg1	diets					318:322	diets	318:322	diets	318:322	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota, were determined.					
35108450	6	83	theme	plasma	1301:1306	arg1	insulin					1308:1314	decreased plasma insulin	1291:1314	decreased plasma insulin	1291:1314	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	3	84	dep	METHODS	432:438	arg1	fed					473:475	fed	473:475	fed	473:475	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	1	85	dep	SCOPE	131:135	arg1	fiber					164:168	prebiotic fiber	154:168	prebiotic fiber	154:168	SCOPE Dietary protein, prebiotic fiber, and exercise individually have been shown to aid in weight loss; however less is known of their combined effects on energy balance.					
35108450	1	85	dep	SCOPE	131:135	arg1	exercise					175:182	exercise	175:182	exercise	175:182	SCOPE Dietary protein, prebiotic fiber, and exercise individually have been shown to aid in weight loss; however less is known of their combined effects on energy balance.					
35108450	1	85	dep	SCOPE	131:135	arg1	SCOPE					131:135	SCOPE Dietary protein, prebiotic fiber, and exercise	131:182	SCOPE Dietary protein, prebiotic fiber, and exercise	131:182	SCOPE Dietary protein, prebiotic fiber, and exercise individually have been shown to aid in weight loss; however less is known of their combined effects on energy balance.					
35108450	1	85	dep	SCOPE	131:135	arg1	protein					145:151	Dietary protein	137:151	Dietary protein	137:151	SCOPE Dietary protein, prebiotic fiber, and exercise individually have been shown to aid in weight loss; however less is known of their combined effects on energy balance.					
35108450	4	86	theme	inulin	689:694	arg1	diets					705:709	inulin enriched diets	689:709	inulin enriched diets	689:709	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	6	87	theme	improved	1204:1211	arg1	control					1222:1228	improved glycemic control	1204:1228	improved glycemic control	1204:1228	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	0	88	theme	Inulin	41:46	arg1	Fiber					48:52	Inulin Fiber	41:52	Inulin Fiber	41:52	Diets Containing Egg or Whey Protein and Inulin Fiber Improve Energy Balance and Modulate Gut Microbiota in Exercising Obese Rats.					
35108450	4	89	from	YY	864:865	arg1	gut					894:896	distal gut	887:896	distal gut	887:896	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
35108450	7	90	theme	plasma	1398:1403	arg1	metabolomics					1405:1416	plasma metabolomics	1398:1416	plasma metabolomics	1398:1416	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	2	91	from	high	324:327	arg1	protein					332:338	protein	332:338	protein	332:338	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota, were determined.					
35108450	2	91	from	high	324:327	arg1	fiber					344:348	fiber	344:348	fiber	344:348	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota, were determined.					
35108450	6	92	theme	decreased	1171:1179	arg1	transcripts					1191:1201	decreased lipogenic transcripts	1171:1201	decreased lipogenic transcripts	1171:1201	Egg-cellulose, egg-inulin, and whey-inulin diets decreased weight gain, adiposity, and hepatic lipidosis; decreased lipogenic transcripts, improved glycemic control, and upregulated hepatic glucose metabolism transcripts; and decreased plasma insulin and leptin.					
35108450	5	93	theme	β-adrenergic	1014:1025	arg1	blockade					1027:1034	β-adrenergic blockade	1014:1034	β-adrenergic blockade	1014:1034	Notably, CCK1-receptor blockade attenuated the hypophagic effects of diets and in particular whey-inulin diet, and β-adrenergic blockade reduced EE across all diets.					
35108450	2	94	from	protein	332:338	arg1	high					324:327	high	324:327	high	324:327	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota, were determined.					
35108450	3	95	theme	egg	561:563	arg1	protein					565:571	egg protein	561:571	egg protein	561:571	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	7	96	theme	gut	1368:1370	arg1	composition					1382:1392	altered gut microbial composition	1360:1392	altered gut microbial composition	1360:1392	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	2	97	from	fiber	344:348	arg1	high					324:327	high	324:327	high	324:327	The effects of diets high in protein and fiber, with exercise, on energy balance, hormones, and gut microbiota, were determined.					
35108450	3	98	from	protein	565:571	arg1	protein					502:508	high protein and fiber contents	497:527	protein	502:508	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	3	98	from	protein	565:571	arg1	contents					520:527	high protein and fiber contents	497:527	contents	520:527	METHODS AND RESULTS Obese male rats were fed high-fat diets with high protein and fiber contents from egg protein and cellulose, egg protein and inulin, whey protein and cellulose, or whey protein and inulin, together with treadmill exercise.					
35108450	7	99	attach	linked	1350:1355	arg1	composition					1382:1392	altered gut microbial composition	1360:1392	altered gut microbial composition	1360:1392	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	7	99	attach	linked	1350:1355	arg1	metabolomics					1405:1416	plasma metabolomics	1398:1416	plasma metabolomics	1398:1416	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	7	99	attach	linked	1350:1355	arg2	diet					1341:1344	diet	1341:1344	diet	1341:1344	Importantly, diet was linked to altered gut microbial composition and plasma metabolomics, and a subset of predicted metagenome pathways and plasma metabolites significantly correlated, with plasma butyric acid the most strongly associated to metagenome function.					
35108450	4	100	from	cholecystokinin	833:847	arg1	gut					894:896	distal gut	887:896	distal gut	887:896	We found that inulin enriched diets decreased energy intake and respiratory quotient (RQ), increased energy expenditure (EE), and upregulated transcripts for cholecystokinin (CCK), peptide YY, and proglucagon in distal gut.					
36974521	5	0	theme	control	1040:1046	arg1	Ob					1030:1031	Ob	1030:1031	Ob	1030:1031	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	0	theme	control	1040:1046	arg1	HC					1075:1076	HC	1075:1076	HC	1075:1076	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	0	theme	control	1040:1046	arg1	group					1048:1052	a control group	1038:1052	a control group of healthy subjects (HC)	1038:1077	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	0	theme	control	1040:1046	arg1	subjects					1065:1072	healthy subjects	1057:1072	healthy subjects (HC)	1057:1077	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	0	theme	control	1040:1046	arg1	patients					1020:1027	obese patients	1014:1027	obese patients (Ob)	1014:1032	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	8	1	theme	quality	1750:1756	arg1	measure					1733:1739	an immediate measure	1720:1739	an immediate measure of their quality	1720:1756	Overall, these results demonstrated that RS is a viable approach for quickly and effectively gaining information on LPs' biochemical composition and oxidation state, providing an immediate measure of their quality.					
36974521	4	2	theme	present	858:864	arg1	lipids					851:856	unsaturated lipids	839:856	unsaturated lipids present in LPs	839:871	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	3	3	theme	overall	481:487	arg1	"					497:497	the overall "quality"	477:497	the overall "quality" of lipoproteins	477:513	However, in recent times, the study of the overall "quality" of lipoproteins, defined by their biochemical composition and oxidation state, has emerged as necessary to improve the definition of the cardiovascular risk.					
36974521	5	4	from	differences	917:927	arg1	composition					948:958	biochemical composition	936:958	biochemical composition	936:958	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	4	from	differences	917:927	arg1	state					974:978	oxidation state	964:978	oxidation state	964:978	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	0	5	theme	obese	70:74	arg1	subjects					88:95	obese and healthy subjects	70:95	obese and healthy subjects	70:95	Determination of the quality of lipoproteins by Raman spectroscopy in obese and healthy subjects.					
36974521	4	6	attach	present	674:680	arg2	we					671:672	we	671:672	we	671:672	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	4	6	attach	present	674:680	arg1	work					665:668	this work	660:668	this work	660:668	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	1	7	theme	lipids	149:154	arg1	complexes					136:144	multimolecular complexes	121:144	multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body	121:272	Lipoproteins (LPs) are multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body.					
36974521	1	7	theme	lipids	149:154	arg1	Lipoproteins					98:109	Lipoproteins	98:109	Lipoproteins (LPs)	98:115	Lipoproteins (LPs) are multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body.					
36974521	7	8	theme	different	1454:1462	arg1	classes					1464:1470	the different classes	1450:1470	the different classes of LPs	1450:1477	RS analysis also allowed the study of the relationship between the levels of carotenoids present in the different classes of LPs highlighting how this parameter depends on the disease severity.					
36974521	6	9	theme	high-density	1252:1263	arg1	HDLs					1279:1282	HDLs	1279:1282	HDLs	1279:1282	In particular, RS revealed how low-density lipoproteins (LDLs) from obese patients are enriched in triglycerides and more oxidized than those from the control group, while high-density lipoproteins (HDLs) from Ob patients were depleted in cholesterol and phospholipids.					
36974521	6	9	theme	high-density	1252:1263	arg1	lipoproteins					1265:1276	high-density lipoproteins	1252:1276	high-density lipoproteins (HDLs) from Ob patients	1252:1300	In particular, RS revealed how low-density lipoproteins (LDLs) from obese patients are enriched in triglycerides and more oxidized than those from the control group, while high-density lipoproteins (HDLs) from Ob patients were depleted in cholesterol and phospholipids.					
36974521	0	10	from	Determination	0:12	arg1	subjects					88:95	obese and healthy subjects	70:95	obese and healthy subjects	70:95	Determination of the quality of lipoproteins by Raman spectroscopy in obese and healthy subjects.					
36974521	3	11	theme	quality	490:496	arg1	"					497:497	the overall "quality"	477:497	the overall "quality" of lipoproteins	477:513	However, in recent times, the study of the overall "quality" of lipoproteins, defined by their biochemical composition and oxidation state, has emerged as necessary to improve the definition of the cardiovascular risk.					
36974521	3	12	theme	risk	651:654	arg1	definition					618:627	the definition	614:627	the definition of the cardiovascular risk	614:654	However, in recent times, the study of the overall "quality" of lipoproteins, defined by their biochemical composition and oxidation state, has emerged as necessary to improve the definition of the cardiovascular risk.					
36974521	6	13	from	patients	1154:1161	arg1	lipoproteins					1123:1134	low-density lipoproteins	1111:1134	low-density lipoproteins (LDLs) from obese patients	1111:1161	In particular, RS revealed how low-density lipoproteins (LDLs) from obese patients are enriched in triglycerides and more oxidized than those from the control group, while high-density lipoproteins (HDLs) from Ob patients were depleted in cholesterol and phospholipids.					
36974521	6	13	from	patients	1154:1161	arg1	LDLs					1137:1140	LDLs	1137:1140	LDLs	1137:1140	In particular, RS revealed how low-density lipoproteins (LDLs) from obese patients are enriched in triglycerides and more oxidized than those from the control group, while high-density lipoproteins (HDLs) from Ob patients were depleted in cholesterol and phospholipids.					
36974521	0	14	theme	healthy	80:86	arg1	subjects					88:95	obese and healthy subjects	70:95	obese and healthy subjects	70:95	Determination of the quality of lipoproteins by Raman spectroscopy in obese and healthy subjects.					
36974521	7	15	theme	RS	1350:1351	arg1	analysis					1353:1360	RS analysis	1350:1360	RS analysis	1350:1360	RS analysis also allowed the study of the relationship between the levels of carotenoids present in the different classes of LPs highlighting how this parameter depends on the disease severity.					
36974521	4	16	from	present	858:864	arg1	LPs					869:871	LPs	869:871	LPs	869:871	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	9	17	theme	LPs	1798:1800	arg1	role					1790:1793	the role	1786:1793	the role of LPs in obesity and metabolic dysfunctions	1786:1838	Besides, RS further proved the role of LPs in obesity and metabolic dysfunctions.					
36974521	4	18	theme	principal	789:797	arg1	components					811:820	the principal biochemical components	785:820	the principal biochemical components	785:820	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	5	19	dep	composition	948:958	arg1	the					932:934	the	932:934	the	932:934	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	20	theme	patients	1020:1027	arg1	group					1048:1052	a control group	1038:1052	a control group of healthy subjects (HC)	1038:1077	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	20	theme	patients	1020:1027	arg1	Ob					1030:1031	Ob	1030:1031	Ob	1030:1031	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	20	theme	patients	1020:1027	arg1	subjects					1065:1072	healthy subjects	1057:1072	healthy subjects (HC)	1057:1077	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	20	theme	patients	1020:1027	arg1	cohort					1004:1009	a cohort	1002:1009	a cohort of obese patients (Ob)	1002:1032	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	20	theme	patients	1020:1027	arg1	HC					1075:1076	HC	1075:1076	HC	1075:1076	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	20	theme	patients	1020:1027	arg1	patients					1020:1027	obese patients	1014:1027	obese patients (Ob)	1014:1032	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	6	21	theme	obese	1148:1152	arg1	patients					1154:1161	obese patients	1148:1161	obese patients	1148:1161	In particular, RS revealed how low-density lipoproteins (LDLs) from obese patients are enriched in triglycerides and more oxidized than those from the control group, while high-density lipoproteins (HDLs) from Ob patients were depleted in cholesterol and phospholipids.					
36974521	8	22	theme	immediate	1723:1731	arg1	measure					1733:1739	an immediate measure	1720:1739	an immediate measure of their quality	1720:1756	Overall, these results demonstrated that RS is a viable approach for quickly and effectively gaining information on LPs' biochemical composition and oxidation state, providing an immediate measure of their quality.					
36974521	3	23	theme	lipoproteins	502:513	arg1	"					497:497	the overall "quality"	477:497	the overall "quality" of lipoproteins	477:513	However, in recent times, the study of the overall "quality" of lipoproteins, defined by their biochemical composition and oxidation state, has emerged as necessary to improve the definition of the cardiovascular risk.					
36974521	1	24	theme	proteins	160:167	arg1	complexes					136:144	multimolecular complexes	121:144	multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body	121:272	Lipoproteins (LPs) are multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body.					
36974521	1	24	theme	proteins	160:167	arg1	Lipoproteins					98:109	Lipoproteins	98:109	Lipoproteins (LPs)	98:115	Lipoproteins (LPs) are multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body.					
36974521	3	25	theme	biochemical	533:543	arg1	composition					545:555	their biochemical composition	527:555	their biochemical composition	527:555	However, in recent times, the study of the overall "quality" of lipoproteins, defined by their biochemical composition and oxidation state, has emerged as necessary to improve the definition of the cardiovascular risk.					
36974521	8	26	theme	viable	1593:1598	arg1	approach					1600:1607	a viable approach	1591:1607	a viable approach for quickly and effectively gaining information on LPs' biochemical composition and oxidation state	1591:1707	Overall, these results demonstrated that RS is a viable approach for quickly and effectively gaining information on LPs' biochemical composition and oxidation state, providing an immediate measure of their quality.					
36974521	8	26	theme	viable	1593:1598	arg1	RS					1585:1586	RS	1585:1586	RS	1585:1586	Overall, these results demonstrated that RS is a viable approach for quickly and effectively gaining information on LPs' biochemical composition and oxidation state, providing an immediate measure of their quality.					
36974521	1	27	theme	responsible	169:179	arg1	complexes					136:144	multimolecular complexes	121:144	multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body	121:272	Lipoproteins (LPs) are multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body.					
36974521	1	27	theme	responsible	169:179	arg1	Lipoproteins					98:109	Lipoproteins	98:109	Lipoproteins (LPs)	98:115	Lipoproteins (LPs) are multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body.					
36974521	7	28	theme	disease	1526:1532	arg1	severity					1534:1541	the disease severity	1522:1541	the disease severity	1522:1541	RS analysis also allowed the study of the relationship between the levels of carotenoids present in the different classes of LPs highlighting how this parameter depends on the disease severity.					
36974521	4	29	theme	biochemical	799:809	arg1	components					811:820	the principal biochemical components	785:820	the principal biochemical components	785:820	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	2	30	theme	triglycerides	297:309	arg1	parameter					373:381	a leading clinical parameter	354:381	a leading clinical parameter to assess the increased risk of cardiovascular events	354:435	The quantification of triglycerides and cholesterol carried by lipoproteins is a leading clinical parameter to assess the increased risk of cardiovascular events.					
36974521	2	30	theme	triglycerides	297:309	arg1	quantification					279:292	The quantification	275:292	The quantification of triglycerides and cholesterol carried by lipoproteins	275:349	The quantification of triglycerides and cholesterol carried by lipoproteins is a leading clinical parameter to assess the increased risk of cardiovascular events.					
36974521	0	31	theme	quality	21:27	arg1	Determination					0:12	Determination	0:12	Determination of the quality of lipoproteins by Raman spectroscopy in obese and healthy subjects.	0:96	Determination of the quality of lipoproteins by Raman spectroscopy in obese and healthy subjects.					
36974521	8	32	theme	oxidation	1693:1701	arg1	state					1703:1707	oxidation state	1693:1707	oxidation state	1693:1707	Overall, these results demonstrated that RS is a viable approach for quickly and effectively gaining information on LPs' biochemical composition and oxidation state, providing an immediate measure of their quality.					
36974521	6	33	theme	control	1231:1237	arg1	group					1239:1243	the control group	1227:1243	the control group	1227:1243	In particular, RS revealed how low-density lipoproteins (LDLs) from obese patients are enriched in triglycerides and more oxidized than those from the control group, while high-density lipoproteins (HDLs) from Ob patients were depleted in cholesterol and phospholipids.					
36974521	4	34	theme	relative	773:780	arg1	groups					766:771	the functional groups	751:771	the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs	751:871	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	5	35	theme	LPs	983:985	arg1	composition					948:958	biochemical composition	936:958	biochemical composition	936:958	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	35	theme	LPs	983:985	arg1	state					974:978	oxidation state	964:978	oxidation state	964:978	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	2	36	theme	cardiovascular	415:428	arg1	events					430:435	cardiovascular events	415:435	cardiovascular events	415:435	The quantification of triglycerides and cholesterol carried by lipoproteins is a leading clinical parameter to assess the increased risk of cardiovascular events.					
36974521	2	37	theme	events	430:435	arg1	risk					407:410	the increased risk	393:410	the increased risk of cardiovascular events	393:435	The quantification of triglycerides and cholesterol carried by lipoproteins is a leading clinical parameter to assess the increased risk of cardiovascular events.					
36974521	4	38	attach	present	858:864	arg1	LPs					869:871	LPs	869:871	LPs	869:871	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	4	38	attach	present	858:864	arg2	lipids					851:856	unsaturated lipids	839:856	unsaturated lipids present in LPs	839:871	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	1	39	theme	fatty	198:202	arg1	acids					204:208	fatty acids	198:208	fatty acids	198:208	Lipoproteins (LPs) are multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body.					
36974521	0	40	theme	lipoproteins	32:43	arg1	quality					21:27	the quality	17:27	the quality of lipoproteins	17:43	Determination of the quality of lipoproteins by Raman spectroscopy in obese and healthy subjects.					
36974521	7	41	theme	carotenoids	1427:1437	arg1	levels					1417:1422	the levels	1413:1422	the levels of carotenoids present in the different classes of LPs highlighting how this parameter depends on the disease severity	1413:1541	RS analysis also allowed the study of the relationship between the levels of carotenoids present in the different classes of LPs highlighting how this parameter depends on the disease severity.					
36974521	9	42	theme	metabolic	1817:1825	arg1	dysfunctions					1827:1838	metabolic dysfunctions	1817:1838	metabolic dysfunctions	1817:1838	Besides, RS further proved the role of LPs in obesity and metabolic dysfunctions.					
36974521	6	43	dep	those	1216:1220	arg1	depleted					1307:1314	depleted	1307:1314	were depleted in cholesterol and phospholipids	1302:1347	In particular, RS revealed how low-density lipoproteins (LDLs) from obese patients are enriched in triglycerides and more oxidized than those from the control group, while high-density lipoproteins (HDLs) from Ob patients were depleted in cholesterol and phospholipids.					
36974521	5	44	theme	healthy	1057:1063	arg1	HC					1075:1076	HC	1075:1076	HC	1075:1076	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	44	theme	healthy	1057:1063	arg1	subjects					1065:1072	healthy subjects	1057:1072	healthy subjects (HC)	1057:1077	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	45	theme	obese	1014:1018	arg1	Ob					1030:1031	Ob	1030:1031	Ob	1030:1031	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	45	theme	obese	1014:1018	arg1	patients					1020:1027	obese patients	1014:1027	obese patients (Ob)	1014:1032	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	4	46	theme	functional	755:764	arg1	groups					766:771	the functional groups	751:771	the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs	751:871	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	2	47	theme	increased	397:405	arg1	risk					407:410	the increased risk	393:410	the increased risk of cardiovascular events	393:435	The quantification of triglycerides and cholesterol carried by lipoproteins is a leading clinical parameter to assess the increased risk of cardiovascular events.					
36974521	0	48	theme	Raman	48:52	arg1	spectroscopy					54:65	Raman spectroscopy	48:65	Raman spectroscopy	48:65	Determination of the quality of lipoproteins by Raman spectroscopy in obese and healthy subjects.					
36974521	5	49	theme	subjects	1065:1072	arg1	group					1048:1052	a control group	1038:1052	a control group of healthy subjects (HC)	1038:1077	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	49	theme	subjects	1065:1072	arg1	Ob					1030:1031	Ob	1030:1031	Ob	1030:1031	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	49	theme	subjects	1065:1072	arg1	subjects					1065:1072	healthy subjects	1057:1072	healthy subjects (HC)	1057:1077	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	49	theme	subjects	1065:1072	arg1	cohort					1004:1009	a cohort	1002:1009	a cohort of obese patients (Ob)	1002:1032	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	49	theme	subjects	1065:1072	arg1	HC					1075:1076	HC	1075:1076	HC	1075:1076	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	5	49	theme	subjects	1065:1072	arg1	patients					1020:1027	obese patients	1014:1027	obese patients (Ob)	1014:1032	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	4	50	theme	lipids	851:856	arg1	components					811:820	the principal biochemical components	785:820	the principal biochemical components	785:820	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	4	50	theme	lipids	851:856	arg1	level					830:834	the level	826:834	the level of unsaturated lipids present in LPs	826:871	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	4	51	from	LPs	869:871	arg1	present					858:864	present	858:864	present	858:864	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	7	52	theme	present	1439:1445	arg1	carotenoids					1427:1437	carotenoids	1427:1437	carotenoids present in the different classes of LPs highlighting how this parameter depends on the disease severity	1427:1541	RS analysis also allowed the study of the relationship between the levels of carotenoids present in the different classes of LPs highlighting how this parameter depends on the disease severity.					
36974521	2	53	theme	leading	356:362	arg1	parameter					373:381	a leading clinical parameter	354:381	a leading clinical parameter to assess the increased risk of cardiovascular events	354:435	The quantification of triglycerides and cholesterol carried by lipoproteins is a leading clinical parameter to assess the increased risk of cardiovascular events.					
36974521	2	53	theme	leading	356:362	arg1	quantification					279:292	The quantification	275:292	The quantification of triglycerides and cholesterol carried by lipoproteins	275:349	The quantification of triglycerides and cholesterol carried by lipoproteins is a leading clinical parameter to assess the increased risk of cardiovascular events.					
36974521	4	54	theme	unsaturated	839:849	arg1	lipids					851:856	unsaturated lipids	839:856	unsaturated lipids present in LPs	839:871	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	3	55	theme	cardiovascular	636:649	arg1	risk					651:654	the cardiovascular risk	632:654	the cardiovascular risk	632:654	However, in recent times, the study of the overall "quality" of lipoproteins, defined by their biochemical composition and oxidation state, has emerged as necessary to improve the definition of the cardiovascular risk.					
36974521	3	56	theme	oxidation	561:569	arg1	state					571:575	oxidation state	561:575	oxidation state	561:575	However, in recent times, the study of the overall "quality" of lipoproteins, defined by their biochemical composition and oxidation state, has emerged as necessary to improve the definition of the cardiovascular risk.					
36974521	6	57	theme	low-density	1111:1121	arg1	lipoproteins					1123:1134	low-density lipoproteins	1111:1134	low-density lipoproteins (LDLs) from obese patients	1111:1161	In particular, RS revealed how low-density lipoproteins (LDLs) from obese patients are enriched in triglycerides and more oxidized than those from the control group, while high-density lipoproteins (HDLs) from Ob patients were depleted in cholesterol and phospholipids.					
36974521	6	57	theme	low-density	1111:1121	arg1	LDLs					1137:1140	LDLs	1137:1140	LDLs	1137:1140	In particular, RS revealed how low-density lipoproteins (LDLs) from obese patients are enriched in triglycerides and more oxidized than those from the control group, while high-density lipoproteins (HDLs) from Ob patients were depleted in cholesterol and phospholipids.					
36974521	6	58	from	patients	1293:1300	arg1	HDLs					1279:1282	HDLs	1279:1282	HDLs	1279:1282	In particular, RS revealed how low-density lipoproteins (LDLs) from obese patients are enriched in triglycerides and more oxidized than those from the control group, while high-density lipoproteins (HDLs) from Ob patients were depleted in cholesterol and phospholipids.					
36974521	6	58	from	patients	1293:1300	arg1	lipoproteins					1265:1276	high-density lipoproteins	1252:1276	high-density lipoproteins (HDLs) from Ob patients	1252:1300	In particular, RS revealed how low-density lipoproteins (LDLs) from obese patients are enriched in triglycerides and more oxidized than those from the control group, while high-density lipoproteins (HDLs) from Ob patients were depleted in cholesterol and phospholipids.					
36974521	1	59	theme	multimolecular	121:134	arg1	complexes					136:144	multimolecular complexes	121:144	multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body	121:272	Lipoproteins (LPs) are multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body.					
36974521	1	59	theme	multimolecular	121:134	arg1	Lipoproteins					98:109	Lipoproteins	98:109	Lipoproteins (LPs)	98:115	Lipoproteins (LPs) are multimolecular complexes of lipids and proteins responsible for transporting fatty acids, cholesterol, and micronutrients (carotenoids) through the body.					
36974521	7	60	theme	relationship	1392:1403	arg1	study					1379:1383	the study	1375:1383	the study of the relationship between the levels of carotenoids present in the different classes of LPs highlighting how this parameter depends on the disease severity	1375:1541	RS analysis also allowed the study of the relationship between the levels of carotenoids present in the different classes of LPs highlighting how this parameter depends on the disease severity.					
36974521	5	61	theme	oxidation	964:972	arg1	state					974:978	oxidation state	964:978	oxidation state	964:978	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	4	62	theme	Raman	682:686	arg1	RS					702:703	RS	702:703	RS	702:703	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	4	62	theme	Raman	682:686	arg1	spectroscopy					688:699	Raman spectroscopy	682:699	Raman spectroscopy (RS)	682:704	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	4	62	theme	Raman	682:686	arg1	method					722:727	an effective method	709:727	an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs	709:871	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	7	63	theme	LPs	1475:1477	arg1	classes					1464:1470	the different classes	1450:1470	the different classes of LPs	1450:1477	RS analysis also allowed the study of the relationship between the levels of carotenoids present in the different classes of LPs highlighting how this parameter depends on the disease severity.					
36974521	4	64	theme	effective	712:720	arg1	spectroscopy					688:699	Raman spectroscopy	682:699	Raman spectroscopy (RS)	682:704	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	4	64	theme	effective	712:720	arg1	method					722:727	an effective method	709:727	an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs	709:871	In this work, we present Raman spectroscopy (RS) as an effective method to immediately detect the functional groups relative to the principal biochemical components and the level of unsaturated lipids present in LPs.					
36974521	8	65	theme	biochemical	1665:1675	arg1	composition					1677:1687	LPs' biochemical composition	1660:1687	LPs' biochemical composition	1660:1687	Overall, these results demonstrated that RS is a viable approach for quickly and effectively gaining information on LPs' biochemical composition and oxidation state, providing an immediate measure of their quality.					
36974521	2	66	theme	cholesterol	315:325	arg1	parameter					373:381	a leading clinical parameter	354:381	a leading clinical parameter to assess the increased risk of cardiovascular events	354:435	The quantification of triglycerides and cholesterol carried by lipoproteins is a leading clinical parameter to assess the increased risk of cardiovascular events.					
36974521	2	66	theme	cholesterol	315:325	arg1	quantification					279:292	The quantification	275:292	The quantification of triglycerides and cholesterol carried by lipoproteins	275:349	The quantification of triglycerides and cholesterol carried by lipoproteins is a leading clinical parameter to assess the increased risk of cardiovascular events.					
36974521	3	67	theme	recent	450:455	arg1	times					457:461	recent times	450:461	recent times	450:461	However, in recent times, the study of the overall "quality" of lipoproteins, defined by their biochemical composition and oxidation state, has emerged as necessary to improve the definition of the cardiovascular risk.					
36974521	5	68	theme	biochemical	936:946	arg1	composition					948:958	biochemical composition	936:958	biochemical composition	936:958	Furthermore, we show how RS can reveal the differences in the biochemical composition and oxidation state of LPs extracted from a cohort of obese patients (Ob) and a control group of healthy subjects (HC).					
36974521	2	69	theme	clinical	364:371	arg1	parameter					373:381	a leading clinical parameter	354:381	a leading clinical parameter to assess the increased risk of cardiovascular events	354:435	The quantification of triglycerides and cholesterol carried by lipoproteins is a leading clinical parameter to assess the increased risk of cardiovascular events.					
36974521	2	69	theme	clinical	364:371	arg1	quantification					279:292	The quantification	275:292	The quantification of triglycerides and cholesterol carried by lipoproteins	275:349	The quantification of triglycerides and cholesterol carried by lipoproteins is a leading clinical parameter to assess the increased risk of cardiovascular events.					
36974521	6	70	theme	Ob	1290:1291	arg1	patients					1293:1300	Ob patients	1290:1300	Ob patients	1290:1300	In particular, RS revealed how low-density lipoproteins (LDLs) from obese patients are enriched in triglycerides and more oxidized than those from the control group, while high-density lipoproteins (HDLs) from Ob patients were depleted in cholesterol and phospholipids.					
36974521	9	71	from	role	1790:1793	arg1	obesity					1805:1811	obesity	1805:1811	obesity	1805:1811	Besides, RS further proved the role of LPs in obesity and metabolic dysfunctions.					
36974521	9	71	from	role	1790:1793	arg1	dysfunctions					1827:1838	metabolic dysfunctions	1817:1838	metabolic dysfunctions	1817:1838	Besides, RS further proved the role of LPs in obesity and metabolic dysfunctions.					
36974521	3	72	theme	"	497:497	arg1	study					468:472	the study	464:472	the study	464:472	However, in recent times, the study of the overall "quality" of lipoproteins, defined by their biochemical composition and oxidation state, has emerged as necessary to improve the definition of the cardiovascular risk.					
35581078	8	0	theme	aqueous	979:985	arg1	medium					987:992	aqueous medium	979:992	aqueous medium (10 mM PBS, pH 7.4)	979:1012	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	4	1	theme	pore	525:528	arg1	diameter					530:537	Average pore diameter	517:537	Average pore diameter of hydrogels with the highest PeMA content (P1:G1)	517:588	Average pore diameter of hydrogels with the highest PeMA content (P1:G1) was 43 μm.					
35581078	7	2	theme	further	959:965	arg1	studies					967:973	further studies	959:973	further studies	959:973	Therefore, P1:G3 hydrogels were chosen and loaded with curcumin for further studies.					
35581078	1	3	theme	antimicrobial	247:259	arg1	curcumin					234:241	curcumin	234:241	curcumin	234:241	The aim of this study was to develop hydrogel wound dressings made of photocrosslinkable pectin and gelatin with pH dependent release of curcumin, an antimicrobial agent.					
35581078	1	3	theme	antimicrobial	247:259	arg1	agent					261:265	an antimicrobial agent	244:265	an antimicrobial agent	244:265	The aim of this study was to develop hydrogel wound dressings made of photocrosslinkable pectin and gelatin with pH dependent release of curcumin, an antimicrobial agent.					
35581078	6	4	theme	reasonable	763:772	arg1	kPa					802:804	22 kPa	799:804	22 kPa	799:804	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	6	4	theme	reasonable	763:772	arg1	modulus					786:792	a reasonable compressive modulus	761:792	a reasonable compressive modulus (ca. 22 kPa)	761:805	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	12	5	dep	loaded	1470:1475	arg1	curcumin					1461:1468	curcumin	1461:1468	curcumin	1461:1468	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	8	6	theme	mM	998:999	arg1	pH					1006:1007	pH 7.4	1006:1011	pH 7.4	1006:1011	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	8	6	theme	mM	998:999	arg1	PBS					1001:1003	10 mM PBS	995:1003	10 mM PBS	995:1003	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	2	7	theme	Methacrylated	268:280	arg1	forms					282:286	Methacrylated forms	268:286	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively)	268:339	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	1	8	theme	photocrosslinkable	167:184	arg1	pectin					186:191	photocrosslinkable pectin	167:191	photocrosslinkable pectin	167:191	The aim of this study was to develop hydrogel wound dressings made of photocrosslinkable pectin and gelatin with pH dependent release of curcumin, an antimicrobial agent.					
35581078	0	9	link	photocrosslinked	39:54	arg1	dressings					86:94	photocrosslinked pectin/gelatin hydrogel wound dressings	39:94	photocrosslinked pectin/gelatin hydrogel wound dressings	39:94	pH responsive release of curcumin from photocrosslinked pectin/gelatin hydrogel wound dressings.					
35581078	3	10	used	used	494:497	arg2	group					510:514	control group	502:514	control group	502:514	Pure GelMA was used as control group.					
35581078	3	10	used	used	494:497	arg2	GelMA					484:488	Pure GelMA	479:488	Pure GelMA	479:488	Pure GelMA was used as control group.					
35581078	12	11	theme	wound	1521:1525	arg1	materials					1536:1544	wound dressing materials	1521:1544	wound dressing materials	1521:1544	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	6	12	from	incubation	741:750	arg1	PBS					755:757	PBS	755:757	PBS	755:757	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	3	13	theme	control	502:508	arg1	group					510:514	control group	502:514	control group	502:514	Pure GelMA was used as control group.					
35581078	3	13	theme	control	502:508	arg1	GelMA					484:488	Pure GelMA	479:488	Pure GelMA	479:488	Pure GelMA was used as control group.					
35581078	0	14	from	dressings	86:94	arg1	release					14:20	pH responsive release	0:20	pH responsive release of curcumin from photocrosslinked pectin/gelatin hydrogel wound dressings.	0:95	pH responsive release of curcumin from photocrosslinked pectin/gelatin hydrogel wound dressings.					
35581078	9	15	theme	basic	1187:1191	arg1	medium					1193:1198	basic medium	1187:1198	basic medium	1187:1198	Since infected wounds have alkaline pH compared to healthy tissue, faster release at basic medium is preferable for wound grafts.					
35581078	1	16	theme	dependent	213:221	arg1	release					223:229	pH dependent release	210:229	pH dependent release of curcumin, an antimicrobial agent	210:265	The aim of this study was to develop hydrogel wound dressings made of photocrosslinkable pectin and gelatin with pH dependent release of curcumin, an antimicrobial agent.					
35581078	8	17	theme	4	1021:1021	arg1	times					1023:1027	times	1023:1027	times	1023:1027	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	6	18	dep	kPa	802:804	arg1	ca.					795:797	ca.	795:797	ca.	795:797	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	4	19	with	hydrogels	542:550	arg1	content					574:580	the highest PeMA content	557:580	the highest PeMA content (P1:G1)	557:588	Average pore diameter of hydrogels with the highest PeMA content (P1:G1) was 43 μm.					
35581078	5	20	dep	%	630:630	arg1	90					628:629	90	628:629	90	628:629	All hydrogels showed about 90% swelling.					
35581078	12	21	theme	promising	1497:1505	arg1	hydrogels					1483:1491	curcumin loaded P1:G3 hydrogels	1461:1491	curcumin loaded P1:G3 hydrogels	1461:1491	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	12	21	theme	promising	1497:1505	arg1	candidates					1507:1516	promising candidates	1497:1516	promising candidates as wound dressing materials	1497:1544	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	0	22	theme	hydrogel	71:78	arg1	dressings					86:94	photocrosslinked pectin/gelatin hydrogel wound dressings	39:94	photocrosslinked pectin/gelatin hydrogel wound dressings	39:94	pH responsive release of curcumin from photocrosslinked pectin/gelatin hydrogel wound dressings.					
35581078	1	23	theme	wound	143:147	arg1	dressings					149:157	hydrogel wound dressings	134:157	hydrogel wound dressings made of photocrosslinkable pectin and gelatin with pH dependent release of curcumin, an antimicrobial agent	134:265	The aim of this study was to develop hydrogel wound dressings made of photocrosslinkable pectin and gelatin with pH dependent release of curcumin, an antimicrobial agent.					
35581078	12	24	theme	loaded	1470:1475	arg1	hydrogels					1483:1491	curcumin loaded P1:G3 hydrogels	1461:1491	curcumin loaded P1:G3 hydrogels	1461:1491	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	12	24	theme	loaded	1470:1475	arg1	candidates					1507:1516	promising candidates	1497:1516	promising candidates as wound dressing materials	1497:1544	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	6	25	theme	preventing	845:854	arg1	ability					856:862	preventing ability	845:862	preventing ability for bacterial penetration	845:888	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	2	26	dep	pectin	291:296	arg1	GelMA					320:324	GelMA	320:324	GelMA	320:324	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	2	26	dep	pectin	291:296	arg1	PeMA					311:314	PeMA	311:314	PeMA	311:314	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	2	27	theme	UV	466:467	arg1	exposure					469:476	UV exposure	466:476	UV exposure	466:476	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	10	28	theme	Disk	1232:1235	arg1	tests					1247:1251	Disk diffusion tests	1232:1251	Disk diffusion tests	1232:1251	Disk diffusion tests proved antibacterial efficacy of the hydrogels against S. aureus and E. coli.					
35581078	9	29	theme	infected	1108:1115	arg1	wounds					1117:1122	infected wounds	1108:1122	infected wounds	1108:1122	Since infected wounds have alkaline pH compared to healthy tissue, faster release at basic medium is preferable for wound grafts.					
35581078	8	30	dep	release	1036:1042	arg1	times					1023:1027	times	1023:1027	times	1023:1027	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	8	31	dep	times	1023:1027	arg1	faster					1029:1034	faster	1029:1034	faster	1029:1034	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	10	32	theme	antibacterial	1260:1272	arg1	efficacy					1274:1281	antibacterial efficacy	1260:1281	antibacterial efficacy of the hydrogels against S. aureus and E. coli	1260:1328	Disk diffusion tests proved antibacterial efficacy of the hydrogels against S. aureus and E. coli.					
35581078	8	33	with	medium	1082:1087	arg1	pH					1094:1095	pH 5.0	1094:1099	pH 5.0	1094:1099	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	2	34	theme	different	392:400	arg1	compositions					402:413	different compositions	392:413	different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA)	392:461	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	0	35	theme	curcumin	25:32	arg1	release					14:20	pH responsive release	0:20	pH responsive release of curcumin from photocrosslinked pectin/gelatin hydrogel wound dressings.	0:95	pH responsive release of curcumin from photocrosslinked pectin/gelatin hydrogel wound dressings.					
35581078	6	36	theme	days	736:739	arg1	incubation					741:750	21 days incubation	733:750	21 days incubation in PBS	733:757	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	12	37	theme	infected	1587:1594	arg1	wounds					1608:1613	infected and chronic wounds	1587:1613	wounds	1608:1613	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	2	38	theme	v/v	433:435	arg1	1:1					416:418	1:1	416:418	1:1	416:418	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	2	38	theme	v/v	433:435	arg1	ratios					437:442	1:2 and 1:3 v/v ratios	421:442	1:2 and 1:3 v/v ratios of PeMA and GelMA	421:460	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	9	39	contain	have	1124:1127	arg1	wounds					1117:1122	infected wounds	1108:1122	infected wounds	1108:1122	Since infected wounds have alkaline pH compared to healthy tissue, faster release at basic medium is preferable for wound grafts.					
35581078	9	39	contain	have	1124:1127	arg2	pH					1138:1139	alkaline pH	1129:1139	alkaline pH	1129:1139	Since infected wounds have alkaline pH compared to healthy tissue, faster release at basic medium is preferable for wound grafts.					
35581078	0	40	theme	photocrosslinked	39:54	arg1	dressings					86:94	photocrosslinked pectin/gelatin hydrogel wound dressings	39:94	photocrosslinked pectin/gelatin hydrogel wound dressings	39:94	pH responsive release of curcumin from photocrosslinked pectin/gelatin hydrogel wound dressings.					
35581078	12	41	theme	wounds	1608:1613	arg1	treatment					1574:1582	the treatment	1570:1582	the treatment of infected and chronic wounds	1570:1613	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	12	42	theme	chronic	1600:1606	arg1	wounds					1608:1613	infected and chronic wounds	1587:1613	wounds	1608:1613	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	10	43	theme	hydrogels	1290:1298	arg1	efficacy					1274:1281	antibacterial efficacy	1260:1281	antibacterial efficacy of the hydrogels against S. aureus and E. coli	1260:1328	Disk diffusion tests proved antibacterial efficacy of the hydrogels against S. aureus and E. coli.					
35581078	6	44	theme	initial	712:718	arg1	weight					720:725	their initial weight	706:725	their initial weight	706:725	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	4	45	theme	hydrogels	542:550	arg1	diameter					530:537	Average pore diameter	517:537	Average pore diameter of hydrogels with the highest PeMA content (P1:G1)	517:588	Average pore diameter of hydrogels with the highest PeMA content (P1:G1) was 43 μm.					
35581078	9	46	theme	faster	1169:1174	arg1	release					1176:1182	faster release	1169:1182	faster release at basic medium	1169:1198	Since infected wounds have alkaline pH compared to healthy tissue, faster release at basic medium is preferable for wound grafts.					
35581078	6	47	theme	compressive	774:784	arg1	kPa					802:804	22 kPa	799:804	22 kPa	799:804	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	6	47	theme	compressive	774:784	arg1	modulus					786:792	a reasonable compressive modulus	761:792	a reasonable compressive modulus (ca. 22 kPa)	761:805	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	4	48	theme	Average	517:523	arg1	diameter					530:537	Average pore diameter	517:537	Average pore diameter of hydrogels with the highest PeMA content (P1:G1)	517:588	Average pore diameter of hydrogels with the highest PeMA content (P1:G1) was 43 μm.					
35581078	2	49	theme	pectin	291:296	arg1	forms					282:286	Methacrylated forms	268:286	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively)	268:339	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	6	50	dep	stability	673:681	arg1	retained					684:691	retained	684:691	retained about 37% of their initial weight after 21 days incubation in PBS	684:757	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	3	51	theme	Pure	479:482	arg1	group					510:514	control group	502:514	control group	502:514	Pure GelMA was used as control group.					
35581078	3	51	theme	Pure	479:482	arg1	GelMA					484:488	Pure GelMA	479:488	Pure GelMA	479:488	Pure GelMA was used as control group.					
35581078	8	52	theme	10	995:996	arg1	mM					998:999	mM	998:999	mM	998:999	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	11	53	theme	Alamar	1345:1350	arg1	assays					1357:1362	Live/Dead and Alamar blue assays	1331:1362	Live/Dead and Alamar blue assays conducted with L929 fibroblasts	1331:1394	Live/Dead and Alamar blue assays conducted with L929 fibroblasts showed cytocompatibility of the hydrogels.					
35581078	9	54	theme	wound	1218:1222	arg1	grafts					1224:1229	wound grafts	1218:1229	wound grafts	1218:1229	Since infected wounds have alkaline pH compared to healthy tissue, faster release at basic medium is preferable for wound grafts.					
35581078	12	55	theme	dressing	1527:1534	arg1	materials					1536:1544	wound dressing materials	1521:1544	wound dressing materials	1521:1544	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	7	56	theme	G3	905:906	arg1	hydrogels					908:916	P1:G3 hydrogels	902:916	P1:G3 hydrogels	902:916	Therefore, P1:G3 hydrogels were chosen and loaded with curcumin for further studies.					
35581078	6	57	theme	highest	665:671	arg1	stability					673:681	the highest stability	661:681	the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS)	661:758	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	2	58	theme	gelatin	302:308	arg1	forms					282:286	Methacrylated forms	268:286	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively)	268:339	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	6	59	theme	oxygen	808:813	arg1	permeability					815:826	oxygen permeability	808:826	oxygen permeability (7.44 mg/mL)	808:839	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	6	59	theme	oxygen	808:813	arg1	mg/mL					834:838	7.44 mg/mL	829:838	7.44 mg/mL	829:838	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	8	60	theme	curcumin	1047:1054	arg1	release					1036:1042	about 4 times faster release	1015:1042	about 4 times faster release of curcumin	1015:1054	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	7	61	theme	P1	902:903	arg1	hydrogels					908:916	P1:G3 hydrogels	902:916	P1:G3 hydrogels	902:916	Therefore, P1:G3 hydrogels were chosen and loaded with curcumin for further studies.					
35581078	1	62	theme	study	113:117	arg1	aim					101:103	The aim	97:103	The aim of this study	97:117	The aim of this study was to develop hydrogel wound dressings made of photocrosslinkable pectin and gelatin with pH dependent release of curcumin, an antimicrobial agent.					
35581078	11	63	theme	Live/Dead	1331:1339	arg1	assays					1357:1362	Live/Dead and Alamar blue assays	1331:1362	Live/Dead and Alamar blue assays conducted with L929 fibroblasts	1331:1394	Live/Dead and Alamar blue assays conducted with L929 fibroblasts showed cytocompatibility of the hydrogels.					
35581078	12	64	theme	G3	1480:1481	arg1	hydrogels					1483:1491	curcumin loaded P1:G3 hydrogels	1461:1491	curcumin loaded P1:G3 hydrogels	1461:1491	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	12	64	theme	G3	1480:1481	arg1	candidates					1507:1516	promising candidates	1497:1516	promising candidates as wound dressing materials	1497:1544	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	6	65	theme	bacterial	868:876	arg1	penetration					878:888	bacterial penetration	868:888	bacterial penetration	868:888	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	5	66	dep	swelling	632:639	arg1	%					630:630	%	630:630	%	630:630	All hydrogels showed about 90% swelling.					
35581078	0	67	theme	wound	80:84	arg1	dressings					86:94	photocrosslinked pectin/gelatin hydrogel wound dressings	39:94	photocrosslinked pectin/gelatin hydrogel wound dressings	39:94	pH responsive release of curcumin from photocrosslinked pectin/gelatin hydrogel wound dressings.					
35581078	1	68	theme	hydrogel	134:141	arg1	dressings					149:157	hydrogel wound dressings	134:157	hydrogel wound dressings made of photocrosslinkable pectin and gelatin with pH dependent release of curcumin, an antimicrobial agent	134:265	The aim of this study was to develop hydrogel wound dressings made of photocrosslinkable pectin and gelatin with pH dependent release of curcumin, an antimicrobial agent.					
35581078	2	69	theme	GelMA	456:460	arg1	1:1					416:418	1:1	416:418	1:1	416:418	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	2	69	theme	GelMA	456:460	arg1	ratios					437:442	1:2 and 1:3 v/v ratios	421:442	1:2 and 1:3 v/v ratios of PeMA and GelMA	421:460	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	2	70	theme	PeMA	447:450	arg1	1:1					416:418	1:1	416:418	1:1	416:418	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	2	70	theme	PeMA	447:450	arg1	ratios					437:442	1:2 and 1:3 v/v ratios	421:442	1:2 and 1:3 v/v ratios of PeMA and GelMA	421:460	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	12	71	theme	P1	1477:1478	arg1	hydrogels					1483:1491	curcumin loaded P1:G3 hydrogels	1461:1491	curcumin loaded P1:G3 hydrogels	1461:1491	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	12	71	theme	P1	1477:1478	arg1	candidates					1507:1516	promising candidates	1497:1516	promising candidates as wound dressing materials	1497:1544	It was concluded that curcumin loaded P1:G3 hydrogels are promising candidates as wound dressing materials to be further tested in the treatment of infected and chronic wounds.					
35581078	8	72	dep	medium	987:992	arg1	pH					1006:1007	pH 7.4	1006:1011	pH 7.4	1006:1011	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	8	72	dep	medium	987:992	arg1	PBS					1001:1003	10 mM PBS	995:1003	10 mM PBS	995:1003	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	4	73	theme	PeMA	569:572	arg1	content					574:580	the highest PeMA content	557:580	the highest PeMA content (P1:G1)	557:588	Average pore diameter of hydrogels with the highest PeMA content (P1:G1) was 43 μm.					
35581078	10	74	theme	diffusion	1237:1245	arg1	tests					1247:1251	Disk diffusion tests	1232:1251	Disk diffusion tests	1232:1251	Disk diffusion tests proved antibacterial efficacy of the hydrogels against S. aureus and E. coli.					
35581078	0	75	theme	responsive	3:12	arg1	release					14:20	pH responsive release	0:20	pH responsive release of curcumin from photocrosslinked pectin/gelatin hydrogel wound dressings.	0:95	pH responsive release of curcumin from photocrosslinked pectin/gelatin hydrogel wound dressings.					
35581078	8	76	located	observed	1060:1067	arg1	medium					987:992	aqueous medium	979:992	aqueous medium (10 mM PBS, pH 7.4)	979:1012	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	8	76	located	observed	1060:1067	arg2	release					1036:1042	about 4 times faster release	1015:1042	about 4 times faster release of curcumin	1015:1054	In aqueous medium (10 mM PBS, pH 7.4), about 4 times faster release of curcumin was observed than that in medium with pH 5.0.					
35581078	2	77	theme	1:2	421:423	arg1	1:1					416:418	1:1	416:418	1:1	416:418	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	2	77	theme	1:2	421:423	arg1	ratios					437:442	1:2 and 1:3 v/v ratios	421:442	1:2 and 1:3 v/v ratios of PeMA and GelMA	421:460	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	9	78	from	medium	1193:1198	arg1	release					1176:1182	faster release	1169:1182	faster release at basic medium	1169:1198	Since infected wounds have alkaline pH compared to healthy tissue, faster release at basic medium is preferable for wound grafts.					
35581078	9	79	theme	alkaline	1129:1136	arg1	pH					1138:1139	alkaline pH	1129:1139	alkaline pH	1129:1139	Since infected wounds have alkaline pH compared to healthy tissue, faster release at basic medium is preferable for wound grafts.					
35581078	4	80	dep	P1	583:584	arg1	G1					586:587	G1	586:587	P1:G1	583:587	Average pore diameter of hydrogels with the highest PeMA content (P1:G1) was 43 μm.					
35581078	1	81	theme	curcumin	234:241	arg1	release					223:229	pH dependent release	210:229	pH dependent release of curcumin, an antimicrobial agent	210:265	The aim of this study was to develop hydrogel wound dressings made of photocrosslinkable pectin and gelatin with pH dependent release of curcumin, an antimicrobial agent.					
35581078	0	82	theme	pectin/gelatin	56:69	arg1	dressings					86:94	photocrosslinked pectin/gelatin hydrogel wound dressings	39:94	photocrosslinked pectin/gelatin hydrogel wound dressings	39:94	pH responsive release of curcumin from photocrosslinked pectin/gelatin hydrogel wound dressings.					
35581078	4	83	dep	content	574:580	arg1	P1					583:584	P1	583:584	P1:G1	583:587	Average pore diameter of hydrogels with the highest PeMA content (P1:G1) was 43 μm.					
35581078	2	84	theme	1:3	429:431	arg1	1:1					416:418	1:1	416:418	1:1	416:418	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	2	84	theme	1:3	429:431	arg1	ratios					437:442	1:2 and 1:3 v/v ratios	421:442	1:2 and 1:3 v/v ratios of PeMA and GelMA	421:460	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	2	85	dep	compositions	402:413	arg1	1:1					416:418	1:1	416:418	1:1	416:418	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	2	85	dep	compositions	402:413	arg1	ratios					437:442	1:2 and 1:3 v/v ratios	421:442	1:2 and 1:3 v/v ratios of PeMA and GelMA	421:460	Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure.					
35581078	11	86	theme	blue	1352:1355	arg1	assays					1357:1362	Live/Dead and Alamar blue assays	1331:1362	Live/Dead and Alamar blue assays conducted with L929 fibroblasts	1331:1394	Live/Dead and Alamar blue assays conducted with L929 fibroblasts showed cytocompatibility of the hydrogels.					
35581078	9	87	theme	healthy	1153:1159	arg1	tissue					1161:1166	healthy tissue	1153:1166	healthy tissue	1153:1166	Since infected wounds have alkaline pH compared to healthy tissue, faster release at basic medium is preferable for wound grafts.					
35581078	6	88	theme	weight	720:725	arg1	weight					720:725	their initial weight	706:725	their initial weight	706:725	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	6	88	theme	weight	720:725	arg1	%					701:701	37%	699:701	37% of their initial weight	699:725	P1:G3 demonstrated the highest stability (retained about 37% of their initial weight after 21 days incubation in PBS), a reasonable compressive modulus (ca. 22 kPa), oxygen permeability (7.44 mg/mL) and preventing ability for bacterial penetration.					
35581078	4	89	theme	highest	561:567	arg1	content					574:580	the highest PeMA content	557:580	the highest PeMA content (P1:G1)	557:588	Average pore diameter of hydrogels with the highest PeMA content (P1:G1) was 43 μm.					
35581078	11	90	theme	hydrogels	1428:1436	arg1	cytocompatibility					1403:1419	cytocompatibility	1403:1419	cytocompatibility of the hydrogels	1403:1436	Live/Dead and Alamar blue assays conducted with L929 fibroblasts showed cytocompatibility of the hydrogels.					
35581078	11	91	theme	L929	1379:1382	arg1	fibroblasts					1384:1394	L929 fibroblasts	1379:1394	L929 fibroblasts	1379:1394	Live/Dead and Alamar blue assays conducted with L929 fibroblasts showed cytocompatibility of the hydrogels.					
35604178	3	0	with	°C	698:699	arg1	stabilization					715:727	membrane stabilization	706:727	membrane stabilization of maltotriose-grafted ε-poly(L-lysine)	706:767	In this work, cryopreservation of human erythrocytes was realized through high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	2	1	from	nature	485:490	arg1	organisms					502:510	living organisms	495:510	living organisms capable of surviving extreme cold and desiccation	495:560	Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation.					
35604178	2	2	theme	much	425:428	arg1	cryoprotectant					459:472	a biocompatible cryoprotectant	443:472	a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation	443:560	Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation.					
35604178	2	2	theme	much	425:428	arg1	attention					430:438	much attention	425:438	much attention	425:438	Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation.					
35604178	4	3	theme	±	950:950	arg1	%					955:955	96.2 ± 3.4%	945:955	96.2 ± 3.4%	945:955	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	1	4	theme	suitable	188:195	arg1	cryoprotectants					197:211	suitable cryoprotectants	188:211	suitable cryoprotectants	188:211	Cryopreservation of human erythrocytes via suitable cryoprotectants is essential for transfusion during emergencies, but the conventional glycerolization method requires a tedious thawing-deglycerolization process.					
35604178	0	5	theme	ε-poly	127:132	arg1	stabilization					90:102	membrane stabilization	81:102	membrane stabilization of maltotriose-grafted ε-poly(L-lysine)	81:142	Cryopreservation of human erythrocytes through high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	6	6	theme	human	1443:1447	arg1	cryopreservation					1454:1469	human cell cryopreservation	1443:1469	human cell cryopreservation	1443:1469	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	5	7	theme	intracellular	1125:1137	arg1	trehalose					1139:1147	high intracellular trehalose	1120:1147	high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions	1120:1248	It has been demonstrated that the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions.					
35604178	5	8	theme	functional	1036:1045	arg1	glycopeptide					1047:1058	the functional glycopeptide	1032:1058	the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions	1032:1248	It has been demonstrated that the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions.					
35604178	4	9	theme	slight	826:831	arg1	impacts					833:839	slight impacts	826:839	slight impacts on morphology and cell functions	826:872	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	0	10	theme	maltotriose-grafted	107:125	arg1	ε-poly					127:132	maltotriose-grafted ε-poly	107:132	maltotriose-grafted ε-poly(L-lysine)	107:142	Cryopreservation of human erythrocytes through high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	0	10	theme	maltotriose-grafted	107:125	arg1	L-lysine					134:141	L-lysine	134:141	L-lysine	134:141	Cryopreservation of human erythrocytes through high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	5	11	theme	biocompatible	1211:1223	arg1	conditions					1239:1248	the biocompatible glycerol-free conditions	1207:1248	the biocompatible glycerol-free conditions	1207:1248	It has been demonstrated that the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions.					
35604178	6	12	theme	hypothermic	1329:1339	arg1	temperature					1341:1351	a hypothermic temperature	1327:1351	a hypothermic temperature	1327:1351	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	1	13	theme	tedious	317:323	arg1	process					351:357	a tedious thawing-deglycerolization process	315:357	a tedious thawing-deglycerolization process	315:357	Cryopreservation of human erythrocytes via suitable cryoprotectants is essential for transfusion during emergencies, but the conventional glycerolization method requires a tedious thawing-deglycerolization process.					
35604178	5	14	theme	glycerol-free	1225:1237	arg1	conditions					1239:1248	the biocompatible glycerol-free conditions	1207:1248	the biocompatible glycerol-free conditions	1207:1248	It has been demonstrated that the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions.					
35604178	3	15	theme	human	597:601	arg1	erythrocytes					603:614	human erythrocytes	597:614	human erythrocytes	597:614	In this work, cryopreservation of human erythrocytes was realized through high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	2	16	theme	due	474:476	arg1	cryoprotectant					459:472	a biocompatible cryoprotectant	443:472	a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation	443:560	Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation.					
35604178	2	16	theme	due	474:476	arg1	attention					430:438	much attention	425:438	much attention	425:438	Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation.					
35604178	4	17	theme	erythrocytes	918:929	arg1	cryosurvival					896:907	the post-storage cryosurvival	879:907	the post-storage cryosurvival of human erythrocytes	879:929	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	4	18	from	impacts	833:839	arg1	morphology					844:853	morphology	844:853	morphology	844:853	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	4	18	from	impacts	833:839	arg1	functions					864:872	cell functions	859:872	cell functions	859:872	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	3	19	theme	erythrocytes	603:614	arg1	cryopreservation					577:592	cryopreservation	577:592	cryopreservation of human erythrocytes	577:614	In this work, cryopreservation of human erythrocytes was realized through high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	6	20	theme	trehalose	1314:1322	arg1	augmentation					1284:1295	augmentation	1284:1295	augmentation of intracellular trehalose	1284:1322	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	5	21	gly	glycopeptide	1047:1058	arg2	glycopeptide					1047:1058	the functional glycopeptide	1032:1058	the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions	1032:1248	It has been demonstrated that the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions.					
35604178	3	22	theme	intracellular	642:654	arg1	trehalose					656:664	high intracellular trehalose	637:664	high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine)	637:767	In this work, cryopreservation of human erythrocytes was realized through high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	2	23	theme	biocompatible	445:457	arg1	cryoprotectant					459:472	a biocompatible cryoprotectant	443:472	a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation	443:560	Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation.					
35604178	2	23	theme	biocompatible	445:457	arg1	attention					430:438	much attention	425:438	much attention	425:438	Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation.					
35604178	2	24	theme	capable	512:518	arg1	organisms					502:510	living organisms	495:510	living organisms capable of surviving extreme cold and desiccation	495:560	Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation.					
35604178	1	25	theme	thawing-deglycerolization	325:349	arg1	process					351:357	a tedious thawing-deglycerolization process	315:357	a tedious thawing-deglycerolization process	315:357	Cryopreservation of human erythrocytes via suitable cryoprotectants is essential for transfusion during emergencies, but the conventional glycerolization method requires a tedious thawing-deglycerolization process.					
35604178	6	26	theme	intracellular	1300:1312	arg1	trehalose					1314:1322	intracellular trehalose	1300:1322	intracellular trehalose	1300:1322	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	4	27	theme	mM	818:819	arg1	±					811:811	94.2 ±	806:811	94.2 ± 12.1 mM	806:819	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	5	28	theme	erythrocytes	1191:1202	arg1	cryopreservation					1165:1180	synergistic cryopreservation	1153:1180	synergistic cryopreservation of human erythrocytes	1153:1202	It has been demonstrated that the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions.					
35604178	0	29	theme	erythrocytes	26:37	arg1	Cryopreservation					0:15	Cryopreservation	0:15	Cryopreservation of human erythrocytes through high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).	0:143	Cryopreservation of human erythrocytes through high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	5	30	dep	demonstrated	1014:1025	arg1	glycopeptide					1047:1058	the functional glycopeptide	1032:1058	the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions	1032:1248	It has been demonstrated that the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions.					
35604178	5	31	from	trehalose	1139:1147	arg1	conditions					1239:1248	the biocompatible glycerol-free conditions	1207:1248	the biocompatible glycerol-free conditions	1207:1248	It has been demonstrated that the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions.					
35604178	2	32	theme	living	495:500	arg1	organisms					502:510	living organisms	495:510	living organisms capable of surviving extreme cold and desiccation	495:560	Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation.					
35604178	4	33	theme	cell	859:862	arg1	functions					864:872	cell functions	859:872	cell functions	859:872	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	5	34	theme	human	1185:1189	arg1	erythrocytes					1191:1202	human erythrocytes	1185:1202	human erythrocytes	1185:1202	It has been demonstrated that the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions.					
35604178	3	35	theme	high	637:640	arg1	trehalose					656:664	high intracellular trehalose	637:664	high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine)	637:767	In this work, cryopreservation of human erythrocytes was realized through high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	4	36	theme	Intracellular	770:782	arg1	trehalose					784:792	Intracellular trehalose	770:792	Intracellular trehalose	770:792	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	0	37	theme	high	47:50	arg1	trehalose					66:74	high intracellular trehalose	47:74	high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine)	47:142	Cryopreservation of human erythrocytes through high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	6	38	theme	functional	1387:1396	arg1	glycopeptide					1398:1409	the functional glycopeptide	1383:1409	the functional glycopeptide	1383:1409	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	3	39	theme	maltotriose-grafted	732:750	arg1	L-lysine					759:766	L-lysine	759:766	L-lysine	759:766	In this work, cryopreservation of human erythrocytes was realized through high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	3	39	theme	maltotriose-grafted	732:750	arg1	ε-poly					752:757	maltotriose-grafted ε-poly	732:757	maltotriose-grafted ε-poly(L-lysine)	732:767	In this work, cryopreservation of human erythrocytes was realized through high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	3	40	theme	benzyl	678:683	arg1	alcohol					685:691	benzyl alcohol	678:691	benzyl alcohol	678:691	In this work, cryopreservation of human erythrocytes was realized through high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	3	41	theme	membrane	706:713	arg1	stabilization					715:727	membrane stabilization	706:727	membrane stabilization of maltotriose-grafted ε-poly(L-lysine)	706:767	In this work, cryopreservation of human erythrocytes was realized through high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	4	42	theme	post-storage	883:894	arg1	cryosurvival					896:907	the post-storage cryosurvival	879:907	the post-storage cryosurvival of human erythrocytes	879:929	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	2	43	theme	extreme	533:539	arg1	cold					541:544	cold	541:544	cold	541:544	Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation.					
35604178	6	44	gly	glycopeptide	1398:1409	arg2	glycopeptide					1398:1409	the functional glycopeptide	1383:1409	the functional glycopeptide	1383:1409	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	4	45	theme	human	912:916	arg1	erythrocytes					918:929	human erythrocytes	912:929	human erythrocytes	912:929	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	4	46	gly	glycopeptide	988:999	arg2	glycopeptide					988:999	the glycopeptide	984:999	the glycopeptide	984:999	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	0	47	theme	intracellular	52:64	arg1	trehalose					66:74	high intracellular trehalose	47:74	high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine)	47:142	Cryopreservation of human erythrocytes through high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	6	48	theme	glycopeptide	1398:1409	arg1	stabilization					1366:1378	membrane stabilization	1357:1378	membrane stabilization	1357:1378	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	6	48	theme	glycopeptide	1398:1409	arg1	temperature					1341:1351	a hypothermic temperature	1327:1351	a hypothermic temperature	1327:1351	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	4	49	theme	membrane	961:968	arg1	protection					970:979	membrane protection	961:979	membrane protection by the glycopeptide	961:999	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	6	50	theme	alternative	1423:1433	arg1	way					1435:1437	an alternative way	1420:1437	an alternative way for human cell cryopreservation	1420:1469	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	6	50	theme	alternative	1423:1433	arg1	approach					1265:1272	This two-step approach	1251:1272	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide	1251:1409	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	6	51	theme	cell	1449:1452	arg1	cryopreservation					1454:1469	human cell cryopreservation	1443:1469	human cell cryopreservation	1443:1469	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	5	52	theme	synergistic	1153:1163	arg1	cryopreservation					1165:1180	synergistic cryopreservation	1153:1180	synergistic cryopreservation of human erythrocytes	1153:1202	It has been demonstrated that the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions.					
35604178	5	53	theme	high	1120:1123	arg1	trehalose					1139:1147	high intracellular trehalose	1120:1147	high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions	1120:1248	It has been demonstrated that the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions.					
35604178	4	54	theme	12.1	813:816	arg1	mM					818:819	mM	818:819	mM	818:819	Intracellular trehalose could reach 94.2 ± 12.1 mM with slight impacts on morphology and cell functions, and the post-storage cryosurvival of human erythrocytes could achieve 96.2 ± 3.4% via membrane protection by the glycopeptide.					
35604178	3	55	theme	ε-poly	752:757	arg1	stabilization					715:727	membrane stabilization	706:727	membrane stabilization of maltotriose-grafted ε-poly(L-lysine)	706:767	In this work, cryopreservation of human erythrocytes was realized through high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	5	56	theme	extracellular	1076:1088	arg1	cryoprotectant					1090:1103	an extracellular cryoprotectant	1073:1103	an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions	1073:1248	It has been demonstrated that the functional glycopeptide performed as an extracellular cryoprotectant accompanied by high intracellular trehalose for synergistic cryopreservation of human erythrocytes in the biocompatible glycerol-free conditions.					
35604178	0	57	with	trehalose	66:74	arg1	stabilization					90:102	membrane stabilization	81:102	membrane stabilization of maltotriose-grafted ε-poly(L-lysine)	81:142	Cryopreservation of human erythrocytes through high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	2	58	theme	nonreducing	388:398	arg1	trehalose					375:383	trehalose	375:383	trehalose	375:383	Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation.					
35604178	2	58	theme	nonreducing	388:398	arg1	disaccharide					400:411	a nonreducing disaccharide	386:411	a nonreducing disaccharide	386:411	Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation.					
35604178	6	59	theme	two-step	1256:1263	arg1	way					1435:1437	an alternative way	1420:1437	an alternative way for human cell cryopreservation	1420:1469	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	6	59	theme	two-step	1256:1263	arg1	approach					1265:1272	This two-step approach	1251:1272	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide	1251:1409	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	0	60	theme	membrane	81:88	arg1	stabilization					90:102	membrane stabilization	81:102	membrane stabilization of maltotriose-grafted ε-poly(L-lysine)	81:142	Cryopreservation of human erythrocytes through high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	6	61	theme	membrane	1357:1364	arg1	stabilization					1366:1378	membrane stabilization	1357:1378	membrane stabilization	1357:1378	This two-step approach involving augmentation of intracellular trehalose at a hypothermic temperature and membrane stabilization of the functional glycopeptide could be an alternative way for human cell cryopreservation.					
35604178	1	62	theme	human	165:169	arg1	erythrocytes					171:182	human erythrocytes	165:182	human erythrocytes	165:182	Cryopreservation of human erythrocytes via suitable cryoprotectants is essential for transfusion during emergencies, but the conventional glycerolization method requires a tedious thawing-deglycerolization process.					
35604178	1	63	theme	conventional	270:281	arg1	method					299:304	the conventional glycerolization method	266:304	the conventional glycerolization method	266:304	Cryopreservation of human erythrocytes via suitable cryoprotectants is essential for transfusion during emergencies, but the conventional glycerolization method requires a tedious thawing-deglycerolization process.					
35604178	0	64	theme	human	20:24	arg1	erythrocytes					26:37	human erythrocytes	20:37	human erythrocytes	20:37	Cryopreservation of human erythrocytes through high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).					
35604178	1	65	theme	erythrocytes	171:182	arg1	Cryopreservation					145:160	Cryopreservation	145:160	Cryopreservation of human erythrocytes via suitable cryoprotectants	145:211	Cryopreservation of human erythrocytes via suitable cryoprotectants is essential for transfusion during emergencies, but the conventional glycerolization method requires a tedious thawing-deglycerolization process.					
35604178	1	66	theme	glycerolization	283:297	arg1	method					299:304	the conventional glycerolization method	266:304	the conventional glycerolization method	266:304	Cryopreservation of human erythrocytes via suitable cryoprotectants is essential for transfusion during emergencies, but the conventional glycerolization method requires a tedious thawing-deglycerolization process.					
36440696	0	0	theme	Mosquito	85:92	arg1	aegypti					100:106	the Mosquito Aedes aegypti	81:106	the Mosquito Aedes aegypti	81:106	Sugar Bait Composition Containing Ivermectin Affect Engorgement and Mortality of the Mosquito Aedes aegypti (Diptera: Culicidae).					
36440696	6	1	dep	h	1104:1104	arg1	feeding					1112:1118	feeding	1112:1118	feeding	1112:1118	Engorgement on the baits was observed under a stereomicroscope, and mortality was followed up to 48 h after feeding.					
36440696	3	2	from	effects	421:427	arg1	baits					467:471	baits	467:471	baits with and without an attractant (concentrated guava juice)	467:529	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	2	3	theme	relationship	324:335	arg1	combination					260:270	the combination	256:270	the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality	256:375	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	6	4	dep	48	1101:1102	arg1	to					1098:1099	to	1098:1099	to	1098:1099	Engorgement on the baits was observed under a stereomicroscope, and mortality was followed up to 48 h after feeding.					
36440696	8	5	theme	IVM	1334:1336	arg1	LC90					1326:1329	the LC90	1322:1329	the LC90 of IVM	1322:1336	A large increase in the LC90 of IVM was observed for females feeding on ATSBs and TSBs with 70% sucrose relative to those feeding on baits with lower sugar concentrations.					
36440696	8	6	with	TSBs	1384:1387	arg1	sucrose					1398:1404	70% sucrose	1394:1404	70% sucrose	1394:1404	A large increase in the LC90 of IVM was observed for females feeding on ATSBs and TSBs with 70% sucrose relative to those feeding on baits with lower sugar concentrations.					
36440696	0	7	theme	aegypti	100:106	arg1	Mortality					68:76	Mortality	68:76	Mortality	68:76	Sugar Bait Composition Containing Ivermectin Affect Engorgement and Mortality of the Mosquito Aedes aegypti (Diptera: Culicidae).					
36440696	0	7	theme	aegypti	100:106	arg1	Engorgement					52:62	Engorgement	52:62	Engorgement	52:62	Sugar Bait Composition Containing Ivermectin Affect Engorgement and Mortality of the Mosquito Aedes aegypti (Diptera: Culicidae).					
36440696	4	8	theme	100	725:727	arg1	ppm					729:731	ppm	729:731	ppm	729:731	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	0	9	dep	Diptera	109:115	arg1	Culicidae					118:126	Culicidae	118:126	Culicidae	118:126	Sugar Bait Composition Containing Ivermectin Affect Engorgement and Mortality of the Mosquito Aedes aegypti (Diptera: Culicidae).					
36440696	7	10	theme	sugar	1235:1239	arg1	concentrations					1217:1230	higher concentrations	1210:1230	higher concentrations of sugar in ATSBs	1210:1248	In general, more mosquitoes engorged on TSB regardless of the sugar concentration, while higher concentrations of sugar in ATSBs resulted in higher numbers of mosquitoes engorging.					
36440696	0	11	theme	Aedes	94:98	arg1	aegypti					100:106	the Mosquito Aedes aegypti	81:106	the Mosquito Aedes aegypti	81:106	Sugar Bait Composition Containing Ivermectin Affect Engorgement and Mortality of the Mosquito Aedes aegypti (Diptera: Culicidae).					
36440696	7	12	dep	mosquitoes	1138:1147	arg1	engorged					1149:1156	engorged	1149:1156	engorged on TSB regardless of the sugar concentration	1149:1201	In general, more mosquitoes engorged on TSB regardless of the sugar concentration, while higher concentrations of sugar in ATSBs resulted in higher numbers of mosquitoes engorging.					
36440696	4	13	theme	toxic	661:665	arg1	ATSB					680:683	ATSB	680:683	ATSB	680:683	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	4	13	theme	toxic	661:665	arg1	baits					673:677	attractant toxic sugar baits	650:677	attractant toxic sugar baits (ATSB)	650:684	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	2	14	with	concentration	281:293	arg1	mortality					367:375	mortality	367:375	mortality	367:375	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	2	14	with	concentration	281:293	arg1	engorgement					351:361	mosquito engorgement	342:361	mosquito engorgement	342:361	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	5	15	theme	lethal	958:963	arg1	concentrations					965:978	lethal concentrations	958:978	the lethal concentrations (LC50 and LC90) values	954:1001	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	5	15	theme	lethal	958:963	arg1	LC90					990:993	LC90	990:993	LC90	990:993	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	5	15	theme	lethal	958:963	arg1	LC50					981:984	LC50	981:984	LC50	981:984	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	7	16	from	concentrations	1217:1230	arg1	ATSBs					1244:1248	ATSBs	1244:1248	ATSBs	1244:1248	In general, more mosquitoes engorged on TSB regardless of the sugar concentration, while higher concentrations of sugar in ATSBs resulted in higher numbers of mosquitoes engorging.					
36440696	5	17	theme	concentrations	965:978	arg1	values					996:1001	the lethal concentrations (LC50 and LC90) values	954:1001	the lethal concentrations (LC50 and LC90) values	954:1001	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	4	18	theme	ppm	729:731	arg1	IVM					745:747	IVM	745:747	IVM	745:747	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	4	18	theme	ppm	729:731	arg1	ivermectin					733:742	100 ppm ivermectin	725:742	100 ppm ivermectin (IVM)	725:748	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	3	19	theme	concentrated	505:516	arg1	juice					524:528	concentrated guava juice	505:528	concentrated guava juice	505:528	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	3	19	theme	concentrated	505:516	arg1	attractant					493:502	an attractant	490:502	an attractant (concentrated guava juice)	490:529	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	0	20	theme	Bait	6:9	arg1	Composition					11:21	Sugar Bait Composition	0:21	Sugar Bait Composition	0:21	Sugar Bait Composition Containing Ivermectin Affect Engorgement and Mortality of the Mosquito Aedes aegypti (Diptera: Culicidae).					
36440696	7	21	theme	mosquitoes	1280:1289	arg1	numbers					1269:1275	higher numbers	1262:1275	higher numbers of mosquitoes engorging	1262:1299	In general, more mosquitoes engorged on TSB regardless of the sugar concentration, while higher concentrations of sugar in ATSBs resulted in higher numbers of mosquitoes engorging.					
36440696	8	22	with	baits	1435:1439	arg1	concentrations					1458:1471	lower sugar concentrations	1446:1471	lower sugar concentrations	1446:1471	A large increase in the LC90 of IVM was observed for females feeding on ATSBs and TSBs with 70% sucrose relative to those feeding on baits with lower sugar concentrations.					
36440696	5	23	theme	different	821:829	arg1	concentrations					831:844	different concentrations	821:844	different concentrations of IVM (0.312-100 ppm) in TSB and ATSB	821:883	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	4	24	theme	Toxic	622:626	arg1	TSB					641:643	TSB	641:643	TSB	641:643	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	4	24	theme	Toxic	622:626	arg1	baits					634:638	Toxic sugar baits	622:638	Toxic sugar baits (TSB)	622:644	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	0	25	theme	Sugar	0:4	arg1	Composition					11:21	Sugar Bait Composition	0:21	Sugar Bait Composition	0:21	Sugar Bait Composition Containing Ivermectin Affect Engorgement and Mortality of the Mosquito Aedes aegypti (Diptera: Culicidae).					
36440696	7	26	theme	higher	1262:1267	arg1	numbers					1269:1275	higher numbers	1262:1275	higher numbers of mosquitoes engorging	1262:1299	In general, more mosquitoes engorged on TSB regardless of the sugar concentration, while higher concentrations of sugar in ATSBs resulted in higher numbers of mosquitoes engorging.					
36440696	4	27	theme	attractant	650:659	arg1	ATSB					680:683	ATSB	680:683	ATSB	680:683	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	4	27	theme	attractant	650:659	arg1	baits					673:677	attractant toxic sugar baits	650:677	attractant toxic sugar baits (ATSB)	650:684	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	3	28	theme	engorgement	535:545	arg1	rates					561:565	engorgement and mortality rates	535:565	rates	561:565	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	5	29	theme	%	939:939	arg1	concentrations					912:925	sucrose concentrations	904:925	sucrose concentrations of 10 and 70%	904:939	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	4	30	theme	sugar	667:671	arg1	ATSB					680:683	ATSB	680:683	ATSB	680:683	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	4	30	theme	sugar	667:671	arg1	baits					673:677	attractant toxic sugar baits	650:677	attractant toxic sugar baits (ATSB)	650:684	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	5	31	theme	sucrose	904:910	arg1	concentrations					912:925	sucrose concentrations	904:925	sucrose concentrations of 10 and 70%	904:939	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	2	32	theme	sugar	275:279	arg1	concentration					281:293	sugar concentration	275:293	sugar concentration	275:293	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	3	33	theme	concentrations	438:451	arg1	effects					421:427	the effects	417:427	the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice)	417:529	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	2	34	theme	concentration	281:293	arg1	combination					260:270	the combination	256:270	the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality	256:375	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	2	35	with	composition	301:311	arg1	mortality					367:375	mortality	367:375	mortality	367:375	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	2	35	with	composition	301:311	arg1	engorgement					351:361	mosquito engorgement	342:361	mosquito engorgement	342:361	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	5	36	theme	IVM	849:851	arg1	concentrations					831:844	different concentrations	821:844	different concentrations of IVM (0.312-100 ppm) in TSB and ATSB	821:883	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	1	37	theme	sugar	183:187	arg1	baits					189:193	sugar baits	183:193	sugar baits	183:193	Toxins and attractants have been studied and used in sugar baits for mosquitoes.					
36440696	8	38	theme	70	1394:1395	arg1	%					1396:1396	%	1396:1396	%	1396:1396	A large increase in the LC90 of IVM was observed for females feeding on ATSBs and TSBs with 70% sucrose relative to those feeding on baits with lower sugar concentrations.					
36440696	4	39	dep	sucrose	713:719	arg1	%					711:711	%	711:711	%	711:711	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	7	40	theme	higher	1210:1215	arg1	concentrations					1217:1230	higher concentrations	1210:1230	higher concentrations of sugar in ATSBs	1210:1248	In general, more mosquitoes engorged on TSB regardless of the sugar concentration, while higher concentrations of sugar in ATSBs resulted in higher numbers of mosquitoes engorging.					
36440696	6	41	from	Engorgement	1004:1014	arg1	baits					1023:1027	the baits	1019:1027	the baits	1019:1027	Engorgement on the baits was observed under a stereomicroscope, and mortality was followed up to 48 h after feeding.					
36440696	3	42	dep	aegypti	582:588	arg1	Diptera					601:607	Diptera	601:607	Diptera	601:607	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	3	42	dep	aegypti	582:588	arg1	Linnaeus					591:598	Linnaeus	591:598	Linnaeus	591:598	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	3	43	theme	present	393:399	arg1	study					401:405	the present study	389:405	the present study	389:405	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	5	44	dep	concentrations	965:978	arg1	concentrations					965:978	lethal concentrations	958:978	the lethal concentrations (LC50 and LC90) values	954:1001	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	5	44	dep	concentrations	965:978	arg1	LC90					990:993	LC90	990:993	LC90	990:993	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	5	44	dep	concentrations	965:978	arg1	LC50					981:984	LC50	981:984	LC50	981:984	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	8	45	with	ATSBs	1374:1378	arg1	sucrose					1398:1404	70% sucrose	1394:1404	70% sucrose	1394:1404	A large increase in the LC90 of IVM was observed for females feeding on ATSBs and TSBs with 70% sucrose relative to those feeding on baits with lower sugar concentrations.					
36440696	4	46	contain	containing	686:695	arg2	sucrose					713:719	10, 50, and 70% sucrose	697:719	10, 50, and 70% sucrose	697:719	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	4	46	contain	containing	686:695	arg1	TSB					641:643	TSB	641:643	TSB	641:643	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	4	46	contain	containing	686:695	arg2	IVM					745:747	IVM	745:747	IVM	745:747	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	4	46	contain	containing	686:695	arg1	baits					634:638	Toxic sugar baits	622:638	Toxic sugar baits (TSB)	622:644	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	4	46	contain	containing	686:695	arg2	ivermectin					733:742	100 ppm ivermectin	725:742	100 ppm ivermectin (IVM)	725:748	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	8	47	theme	large	1304:1308	arg1	increase					1310:1317	A large increase	1302:1317	A large increase in the LC90 of IVM	1302:1336	A large increase in the LC90 of IVM was observed for females feeding on ATSBs and TSBs with 70% sucrose relative to those feeding on baits with lower sugar concentrations.					
36440696	0	48	dep	Affect	45:50	arg1	Diptera					109:115	Diptera	109:115	Diptera	109:115	Sugar Bait Composition Containing Ivermectin Affect Engorgement and Mortality of the Mosquito Aedes aegypti (Diptera: Culicidae).					
36440696	8	49	theme	%	1396:1396	arg1	sucrose					1398:1404	70% sucrose	1394:1404	70% sucrose	1394:1404	A large increase in the LC90 of IVM was observed for females feeding on ATSBs and TSBs with 70% sucrose relative to those feeding on baits with lower sugar concentrations.					
36440696	9	50	theme	such	1477:1480	arg1	effect					1482:1487	No such effect	1474:1487	No such effect	1474:1487	No such effect was observed for males.					
36440696	2	51	with	relationship	324:335	arg1	mortality					367:375	mortality	367:375	mortality	367:375	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	2	51	with	relationship	324:335	arg1	engorgement					351:361	mosquito engorgement	342:361	mosquito engorgement	342:361	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	3	52	theme	mortality	551:559	arg1	rates					561:565	engorgement and mortality rates	535:565	rates	561:565	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	8	53	theme	sugar	1452:1456	arg1	concentrations					1458:1471	lower sugar concentrations	1446:1471	lower sugar concentrations	1446:1471	A large increase in the LC90 of IVM was observed for females feeding on ATSBs and TSBs with 70% sucrose relative to those feeding on baits with lower sugar concentrations.					
36440696	2	54	theme	little	220:225	arg1	importance					227:236	little importance	220:236	little importance	220:236	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	4	55	theme	mortality	790:798	arg1	rates					800:804	mortality rates	790:804	mortality rates	790:804	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	3	56	theme	aegypti	582:588	arg1	rates					561:565	engorgement and mortality rates	535:565	rates	561:565	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	1	57	used	used	175:178	arg2	Toxins					130:135	Toxins	130:135	Toxins	130:135	Toxins and attractants have been studied and used in sugar baits for mosquitoes.					
36440696	2	58	theme	composition	301:311	arg1	combination					260:270	the combination	256:270	the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality	256:375	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	8	59	theme	lower	1446:1450	arg1	concentrations					1458:1471	lower sugar concentrations	1446:1471	lower sugar concentrations	1446:1471	A large increase in the LC90 of IVM was observed for females feeding on ATSBs and TSBs with 70% sucrose relative to those feeding on baits with lower sugar concentrations.					
36440696	3	60	dep	Linnaeus	591:598	arg1	Culicidae					610:618	Culicidae	610:618	Culicidae	610:618	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	3	61	theme	guava	518:522	arg1	juice					524:528	concentrated guava juice	505:528	concentrated guava juice	505:528	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	3	61	theme	guava	518:522	arg1	attractant					493:502	an attractant	490:502	an attractant (concentrated guava juice)	490:529	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	2	62	theme	bait	296:299	arg1	composition					301:311	bait composition	296:311	bait composition	296:311	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	3	63	theme	sucrose	456:462	arg1	concentrations					438:451	three concentrations	432:451	three concentrations of sucrose	432:462	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	4	64	dep	%	711:711	arg1	50					701:702	50	701:702	50	701:702	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	4	64	dep	%	711:711	arg1	70					709:710	70	709:710	70	709:710	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	3	65	theme	adult	570:574	arg1	aegypti					582:588	adult Aedes aegypti	570:588	adult Aedes aegypti (Linnaeus, Diptera: Culicidae)	570:619	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	2	66	theme	mosquito	342:349	arg1	engorgement					351:361	mosquito engorgement	342:361	mosquito engorgement	342:361	However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality.					
36440696	5	67	from	concentrations	831:844	arg1	ATSB					880:883	ATSB	880:883	ATSB	880:883	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	5	67	from	concentrations	831:844	arg1	TSB					872:874	TSB	872:874	TSB	872:874	Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values.					
36440696	7	68	theme	sugar	1183:1187	arg1	concentration					1189:1201	the sugar concentration	1179:1201	the sugar concentration	1179:1201	In general, more mosquitoes engorged on TSB regardless of the sugar concentration, while higher concentrations of sugar in ATSBs resulted in higher numbers of mosquitoes engorging.					
36440696	3	69	theme	Aedes	576:580	arg1	aegypti					582:588	adult Aedes aegypti	570:588	adult Aedes aegypti (Linnaeus, Diptera: Culicidae)	570:619	Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae).					
36440696	4	70	theme	sugar	628:632	arg1	TSB					641:643	TSB	641:643	TSB	641:643	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	4	70	theme	sugar	628:632	arg1	baits					634:638	Toxic sugar baits	622:638	Toxic sugar baits (TSB)	622:644	Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates.					
36440696	8	71	from	increase	1310:1317	arg1	LC90					1326:1329	the LC90	1322:1329	the LC90 of IVM	1322:1336	A large increase in the LC90 of IVM was observed for females feeding on ATSBs and TSBs with 70% sucrose relative to those feeding on baits with lower sugar concentrations.					
35848445	0	0	theme	protein	84:90	arg1	cross-linking					92:104	whey protein cross-linking	79:104	whey protein cross-linking	79:104	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	7	1	theme	Alginate	1223:1230	arg1	lyase					1232:1236	Alginate lyase	1223:1236	Alginate lyase added to the intestinal phase	1223:1266	Alginate lyase added to the intestinal phase enhanced digestion (9%) as controlled by alginate composition and enzyme specificity.					
35848445	6	2	theme	human	1111:1115	arg1	enzymes					1117:1123	human enzymes	1111:1123	human enzymes	1111:1123	While alginate is not degraded by human enzymes, a few gut bacteria were recently found to encode lyases and other enzymes metabolizing alginate.					
35848445	0	3	theme	whey	79:82	arg1	cross-linking					92:104	whey protein cross-linking	79:104	whey protein cross-linking	79:104	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	8	4	theme	food	1496:1499	arg1	additives					1501:1509	food additives	1496:1509	food additives	1496:1509	Thus we provide evidence that use of hydrocolloids and processing of protein strongly influence digestion and should be considered when using food additives.					
35848445	1	5	theme	whey	168:171	arg1	Alginate					155:162	Alginate	155:162	Alginate	155:162	Alginate and whey protein are common additives in food production improving storage stability, texture and nutritional value.					
35848445	1	5	theme	whey	168:171	arg1	protein					173:179	whey protein	168:179	whey protein	168:179	Alginate and whey protein are common additives in food production improving storage stability, texture and nutritional value.					
35848445	1	5	theme	whey	168:171	arg1	additives					192:200	common additives	185:200	common additives in food production improving storage stability, texture and nutritional value	185:278	Alginate and whey protein are common additives in food production improving storage stability, texture and nutritional value.					
35848445	1	6	theme	nutritional	262:272	arg1	value					274:278	nutritional value	262:278	nutritional value	262:278	Alginate and whey protein are common additives in food production improving storage stability, texture and nutritional value.					
35848445	6	7	theme	gut	1132:1134	arg1	lyases					1175:1180	lyases	1175:1180	lyases	1175:1180	While alginate is not degraded by human enzymes, a few gut bacteria were recently found to encode lyases and other enzymes metabolizing alginate.					
35848445	6	7	theme	gut	1132:1134	arg1	enzymes					1192:1198	other enzymes	1186:1198	other enzymes metabolizing alginate	1186:1220	While alginate is not degraded by human enzymes, a few gut bacteria were recently found to encode lyases and other enzymes metabolizing alginate.					
35848445	6	7	theme	gut	1132:1134	arg1	bacteria					1136:1143	a few gut bacteria	1126:1143	a few gut bacteria	1126:1143	While alginate is not degraded by human enzymes, a few gut bacteria were recently found to encode lyases and other enzymes metabolizing alginate.					
35848445	0	8	dep	composition	114:124	arg1	the					110:112	the	110:112	the	110:112	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	0	9	theme	cross-linking	92:104	arg1	effects					68:74	effects	68:74	effects of whey protein cross-linking	68:104	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	0	9	theme	cross-linking	92:104	arg1	degradation					130:140	degradation	130:140	degradation	130:140	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	0	9	theme	cross-linking	92:104	arg1	composition					114:124	composition	114:124	composition	114:124	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	4	10	theme	gastrointestinal	660:675	arg1	digestion					677:685	The INFOGEST in vitro simulated gastrointestinal digestion	628:685	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein	628:701	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	7	11	theme	intestinal	1251:1260	arg1	phase					1262:1266	the intestinal phase	1247:1266	the intestinal phase	1247:1266	Alginate lyase added to the intestinal phase enhanced digestion (9%) as controlled by alginate composition and enzyme specificity.					
35848445	5	12	theme	protein	877:883	arg1	particles					885:893	alginate whey protein particles	863:893	alginate whey protein particles	863:893	Sizes of alginate whey protein particles increased during gastric digestion, whereas for cross-linked whey protein complexes the size initially increased, but returned to their initial size at the end of gastric digestion.					
35848445	3	13	theme	whey	480:483	arg1	cross-linking					493:505	whey protein cross-linking	480:505	whey protein cross-linking by microbial transglutaminase	480:535	This study shows that whey protein cross-linking by microbial transglutaminase dramatically decreased particle size (2-fold) and viscosity of alginate protein complexes.					
35848445	5	14	theme	whey	956:959	arg1	complexes					969:977	cross-linked whey protein complexes	943:977	cross-linked whey protein complexes	943:977	Sizes of alginate whey protein particles increased during gastric digestion, whereas for cross-linked whey protein complexes the size initially increased, but returned to their initial size at the end of gastric digestion.					
35848445	2	15	theme	protein	402:408	arg1	complexation					410:421	alginate protein complexation	393:421	alginate protein complexation	393:421	Alginate forms complexes with whey protein and inhibits proteolysis by pepsin and trypsin, but the influence of alginate protein complexation on digestion is poorly understood.					
35848445	6	16	theme	few	1128:1130	arg1	lyases					1175:1180	lyases	1175:1180	lyases	1175:1180	While alginate is not degraded by human enzymes, a few gut bacteria were recently found to encode lyases and other enzymes metabolizing alginate.					
35848445	6	16	theme	few	1128:1130	arg1	enzymes					1192:1198	other enzymes	1186:1198	other enzymes metabolizing alginate	1186:1220	While alginate is not degraded by human enzymes, a few gut bacteria were recently found to encode lyases and other enzymes metabolizing alginate.					
35848445	6	16	theme	few	1128:1130	arg1	bacteria					1136:1143	a few gut bacteria	1126:1143	a few gut bacteria	1126:1143	While alginate is not degraded by human enzymes, a few gut bacteria were recently found to encode lyases and other enzymes metabolizing alginate.					
35848445	4	17	theme	mannuronic	794:803	arg1	acid					805:808	high mannuronic acid	789:808	high mannuronic acid	789:808	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	2	18	theme	alginate	393:400	arg1	complexation					410:421	alginate protein complexation	393:421	alginate protein complexation	393:421	Alginate forms complexes with whey protein and inhibits proteolysis by pepsin and trypsin, but the influence of alginate protein complexation on digestion is poorly understood.					
35848445	0	19	theme	gastrointestinal	10:25	arg1	digestion					27:35	Simulated gastrointestinal digestion	0:35	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.	0:153	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	1	20	theme	common	185:190	arg1	Alginate					155:162	Alginate	155:162	Alginate	155:162	Alginate and whey protein are common additives in food production improving storage stability, texture and nutritional value.					
35848445	1	20	theme	common	185:190	arg1	protein					173:179	whey protein	168:179	whey protein	168:179	Alginate and whey protein are common additives in food production improving storage stability, texture and nutritional value.					
35848445	1	20	theme	common	185:190	arg1	additives					192:200	common additives	185:200	common additives in food production improving storage stability, texture and nutritional value	185:278	Alginate and whey protein are common additives in food production improving storage stability, texture and nutritional value.					
35848445	0	21	theme	Simulated	0:8	arg1	digestion					27:35	Simulated gastrointestinal digestion	0:35	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.	0:153	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	3	22	theme	alginate	600:607	arg1	complexes					617:625	alginate protein complexes	600:625	alginate protein complexes	600:625	This study shows that whey protein cross-linking by microbial transglutaminase dramatically decreased particle size (2-fold) and viscosity of alginate protein complexes.					
35848445	5	23	theme	initial	1031:1037	arg1	size					1039:1042	their initial size	1025:1042	their initial size	1025:1042	Sizes of alginate whey protein particles increased during gastric digestion, whereas for cross-linked whey protein complexes the size initially increased, but returned to their initial size at the end of gastric digestion.					
35848445	8	24	theme	hydrocolloids	1391:1403	arg1	processing					1409:1418	processing	1409:1418	processing of protein	1409:1429	Thus we provide evidence that use of hydrocolloids and processing of protein strongly influence digestion and should be considered when using food additives.					
35848445	8	24	theme	hydrocolloids	1391:1403	arg1	use					1384:1386	use	1384:1386	use of hydrocolloids	1384:1403	Thus we provide evidence that use of hydrocolloids and processing of protein strongly influence digestion and should be considered when using food additives.					
35848445	4	25	theme	in	641:642	arg1	digestion					677:685	The INFOGEST in vitro simulated gastrointestinal digestion	628:685	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein	628:701	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	6	26	theme	other	1186:1190	arg1	enzymes					1192:1198	other enzymes	1186:1198	other enzymes metabolizing alginate	1186:1220	While alginate is not degraded by human enzymes, a few gut bacteria were recently found to encode lyases and other enzymes metabolizing alginate.					
35848445	6	26	theme	other	1186:1190	arg1	bacteria					1136:1143	a few gut bacteria	1126:1143	a few gut bacteria	1126:1143	While alginate is not degraded by human enzymes, a few gut bacteria were recently found to encode lyases and other enzymes metabolizing alginate.					
35848445	2	27	theme	whey	311:314	arg1	protein					316:322	whey protein	311:322	whey protein	311:322	Alginate forms complexes with whey protein and inhibits proteolysis by pepsin and trypsin, but the influence of alginate protein complexation on digestion is poorly understood.					
35848445	1	28	theme	food	205:208	arg1	production					210:219	food production	205:219	food production improving storage stability, texture and nutritional value	205:278	Alginate and whey protein are common additives in food production improving storage stability, texture and nutritional value.					
35848445	0	29	theme	alginate	48:55	arg1	complexes					57:65	protein alginate complexes	40:65	protein alginate complexes	40:65	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	5	30	theme	cross-linked	943:954	arg1	complexes					969:977	cross-linked whey protein complexes	943:977	cross-linked whey protein complexes	943:977	Sizes of alginate whey protein particles increased during gastric digestion, whereas for cross-linked whey protein complexes the size initially increased, but returned to their initial size at the end of gastric digestion.					
35848445	0	31	theme	alginate	145:152	arg1	effects					68:74	effects	68:74	effects of whey protein cross-linking	68:104	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	0	31	theme	alginate	145:152	arg1	degradation					130:140	degradation	130:140	degradation	130:140	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	0	31	theme	alginate	145:152	arg1	composition					114:124	composition	114:124	composition	114:124	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	5	32	theme	alginate	863:870	arg1	particles					885:893	alginate whey protein particles	863:893	alginate whey protein particles	863:893	Sizes of alginate whey protein particles increased during gastric digestion, whereas for cross-linked whey protein complexes the size initially increased, but returned to their initial size at the end of gastric digestion.					
35848445	4	33	theme	acid	840:843	arg1	content					845:851	high guluronic acid content	825:851	high guluronic acid content	825:851	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	4	34	theme	whey	690:693	arg1	protein					695:701	whey protein	690:701	whey protein	690:701	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	0	35	theme	protein	40:46	arg1	complexes					57:65	protein alginate complexes	40:65	protein alginate complexes	40:65	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	4	36	with	pronounced	773:782	arg1	least					814:818	least	814:818	least	814:818	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	4	36	with	pronounced	773:782	arg1	acid					805:808	high mannuronic acid	789:808	high mannuronic acid	789:808	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	4	37	theme	guluronic	830:838	arg1	content					845:851	high guluronic acid content	825:851	high guluronic acid content	825:851	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	2	38	theme	complexation	410:421	arg1	influence					380:388	the influence	376:388	the influence of alginate protein complexation on digestion	376:434	Alginate forms complexes with whey protein and inhibits proteolysis by pepsin and trypsin, but the influence of alginate protein complexation on digestion is poorly understood.					
35848445	3	39	theme	protein	609:615	arg1	complexes					617:625	alginate protein complexes	600:625	alginate protein complexes	600:625	This study shows that whey protein cross-linking by microbial transglutaminase dramatically decreased particle size (2-fold) and viscosity of alginate protein complexes.					
35848445	2	40	from	influence	380:388	arg1	digestion					426:434	digestion	426:434	digestion	426:434	Alginate forms complexes with whey protein and inhibits proteolysis by pepsin and trypsin, but the influence of alginate protein complexation on digestion is poorly understood.					
35848445	3	41	dep	size	569:572	arg1	2-fold					575:580	2-fold	575:580	2-fold	575:580	This study shows that whey protein cross-linking by microbial transglutaminase dramatically decreased particle size (2-fold) and viscosity of alginate protein complexes.					
35848445	0	42	theme	complexes	57:65	arg1	digestion					27:35	Simulated gastrointestinal digestion	0:35	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.	0:153	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	5	43	theme	gastric	1058:1064	arg1	digestion					1066:1074	gastric digestion	1058:1074	gastric digestion	1058:1074	Sizes of alginate whey protein particles increased during gastric digestion, whereas for cross-linked whey protein complexes the size initially increased, but returned to their initial size at the end of gastric digestion.					
35848445	3	44	theme	complexes	617:625	arg1	viscosity					587:595	viscosity	587:595	viscosity of alginate protein complexes	587:625	This study shows that whey protein cross-linking by microbial transglutaminase dramatically decreased particle size (2-fold) and viscosity of alginate protein complexes.					
35848445	3	44	theme	complexes	617:625	arg1	size					569:572	particle size	560:572	particle size (2-fold)	560:581	This study shows that whey protein cross-linking by microbial transglutaminase dramatically decreased particle size (2-fold) and viscosity of alginate protein complexes.					
35848445	7	45	theme	enzyme	1334:1339	arg1	specificity					1341:1351	enzyme specificity	1334:1351	enzyme specificity	1334:1351	Alginate lyase added to the intestinal phase enhanced digestion (9%) as controlled by alginate composition and enzyme specificity.					
35848445	3	46	theme	microbial	510:518	arg1	transglutaminase					520:535	microbial transglutaminase	510:535	microbial transglutaminase	510:535	This study shows that whey protein cross-linking by microbial transglutaminase dramatically decreased particle size (2-fold) and viscosity of alginate protein complexes.					
35848445	5	47	theme	digestion	1066:1074	arg1	end					1051:1053	the end	1047:1053	the end of gastric digestion	1047:1074	Sizes of alginate whey protein particles increased during gastric digestion, whereas for cross-linked whey protein complexes the size initially increased, but returned to their initial size at the end of gastric digestion.					
35848445	8	48	theme	protein	1423:1429	arg1	processing					1409:1418	processing	1409:1418	processing of protein	1409:1429	Thus we provide evidence that use of hydrocolloids and processing of protein strongly influence digestion and should be considered when using food additives.					
35848445	8	48	theme	protein	1423:1429	arg1	use					1384:1386	use	1384:1386	use of hydrocolloids	1384:1403	Thus we provide evidence that use of hydrocolloids and processing of protein strongly influence digestion and should be considered when using food additives.					
35848445	3	49	theme	protein	485:491	arg1	cross-linking					493:505	whey protein cross-linking	480:505	whey protein cross-linking by microbial transglutaminase	480:535	This study shows that whey protein cross-linking by microbial transglutaminase dramatically decreased particle size (2-fold) and viscosity of alginate protein complexes.					
35848445	1	50	theme	storage	231:237	arg1	stability					239:247	storage stability	231:247	storage stability	231:247	Alginate and whey protein are common additives in food production improving storage stability, texture and nutritional value.					
35848445	5	51	link	cross-linked	943:954	arg1	complexes					969:977	cross-linked whey protein complexes	943:977	cross-linked whey protein complexes	943:977	Sizes of alginate whey protein particles increased during gastric digestion, whereas for cross-linked whey protein complexes the size initially increased, but returned to their initial size at the end of gastric digestion.					
35848445	4	52	dep	in	641:642	arg1	vitro					644:648	vitro	644:648	vitro	644:648	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	0	53	dep	digestion	27:35	arg1	effects					68:74	effects	68:74	effects of whey protein cross-linking	68:104	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	0	53	dep	digestion	27:35	arg1	composition					114:124	composition	114:124	composition	114:124	Simulated gastrointestinal digestion of protein alginate complexes: effects of whey protein cross-linking and the composition and degradation of alginate.					
35848445	5	54	theme	protein	961:967	arg1	complexes					969:977	cross-linked whey protein complexes	943:977	cross-linked whey protein complexes	943:977	Sizes of alginate whey protein particles increased during gastric digestion, whereas for cross-linked whey protein complexes the size initially increased, but returned to their initial size at the end of gastric digestion.					
35848445	7	55	theme	alginate	1309:1316	arg1	composition					1318:1328	alginate composition	1309:1328	alginate composition	1309:1328	Alginate lyase added to the intestinal phase enhanced digestion (9%) as controlled by alginate composition and enzyme specificity.					
35848445	4	56	theme	INFOGEST	632:639	arg1	digestion					677:685	The INFOGEST in vitro simulated gastrointestinal digestion	628:685	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein	628:701	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	5	57	theme	whey	872:875	arg1	particles					885:893	alginate whey protein particles	863:893	alginate whey protein particles	863:893	Sizes of alginate whey protein particles increased during gastric digestion, whereas for cross-linked whey protein complexes the size initially increased, but returned to their initial size at the end of gastric digestion.					
35848445	4	58	theme	high	825:828	arg1	content					845:851	high guluronic acid content	825:851	high guluronic acid content	825:851	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	1	59	from	additives	192:200	arg1	production					210:219	food production	205:219	food production improving storage stability, texture and nutritional value	205:278	Alginate and whey protein are common additives in food production improving storage stability, texture and nutritional value.					
35848445	5	60	theme	gastric	912:918	arg1	digestion					920:928	gastric digestion	912:928	gastric digestion	912:928	Sizes of alginate whey protein particles increased during gastric digestion, whereas for cross-linked whey protein complexes the size initially increased, but returned to their initial size at the end of gastric digestion.					
35848445	4	61	theme	protein	695:701	arg1	digestion					677:685	The INFOGEST in vitro simulated gastrointestinal digestion	628:685	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein	628:701	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	4	62	theme	simulated	650:658	arg1	digestion					677:685	The INFOGEST in vitro simulated gastrointestinal digestion	628:685	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein	628:701	The INFOGEST in vitro simulated gastrointestinal digestion of whey protein was increased by cross-linking (16%) and suppressed by alginate, most pronounced with high mannuronic acid and least with high guluronic acid content.					
35848445	5	63	theme	particles	885:893	arg1	Sizes					854:858	Sizes	854:858	Sizes of alginate whey protein particles	854:893	Sizes of alginate whey protein particles increased during gastric digestion, whereas for cross-linked whey protein complexes the size initially increased, but returned to their initial size at the end of gastric digestion.					
35848445	3	64	theme	particle	560:567	arg1	size					569:572	particle size	560:572	particle size (2-fold)	560:581	This study shows that whey protein cross-linking by microbial transglutaminase dramatically decreased particle size (2-fold) and viscosity of alginate protein complexes.					
35848445	2	65	with	complexes	296:304	arg1	protein					316:322	whey protein	311:322	whey protein	311:322	Alginate forms complexes with whey protein and inhibits proteolysis by pepsin and trypsin, but the influence of alginate protein complexation on digestion is poorly understood.					
36192613	14	0	theme	storage	2348:2354	arg1	stability					2356:2364	the highest storage stability	2336:2364	the highest storage stability	2336:2364	• A glassy matrix containing maltodextrin and an antioxidant ensured the highest storage stability.					
36192613	2	1	theme	protective	365:374	arg1	molecules					376:384	various protective molecules	357:384	various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides)	357:452	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	2	1	theme	protective	365:374	arg1	trehalose					401:409	trehalose	401:409	trehalose	401:409	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	2	1	theme	protective	365:374	arg1	maltodextrin					387:398	maltodextrin	387:398	maltodextrin	387:398	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	3	2	theme	infrared	741:748	arg1	micro-spectroscopy					757:774	infrared (FTIR) micro-spectroscopy	741:774	infrared (FTIR) micro-spectroscopy	741:774	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	10	3	theme	bacteria	1864:1871	arg1	mechanisms					1837:1846	the stabilization mechanisms	1819:1846	the stabilization mechanisms of freeze-dried bacteria	1819:1871	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	9	4	theme	protective	1514:1523	arg1	molecules					1525:1533	the protective molecules	1510:1533	the protective molecules	1510:1533	Storage stability appeared to be dependent on the ability of the protective molecules to limit damage during freeze-drying.					
36192613	8	5	theme	FTIR	1253:1256	arg1	spectra					1258:1264	the FTIR spectra	1249:1264	the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage	1249:1340	The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage revealed that freeze-drying induced damage to proteins, peptidoglycans of the cell wall and nucleic acids.					
36192613	7	6	theme	storage	1140:1146	arg1	stability					1148:1156	The best storage stability	1131:1156	The best storage stability	1131:1156	The best storage stability was observed for the formulations involving maltodextrin and antioxidants.					
36192613	3	7	theme	freeze-drying	595:607	arg1	storage					625:631	freezing, freeze-drying, and subsequent storage	585:631	freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment	585:715	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	10	8	theme	difference	1670:1679	arg1	rates					1590:1594	The inactivation rates	1573:1594	The inactivation rates of bacteria during storage	1573:1621	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	10	8	theme	difference	1670:1679	arg1	function					1642:1649	a function	1640:1649	a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature	1640:1785	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	3	9	theme	freezing	585:592	arg1	storage					625:631	freezing, freeze-drying, and subsequent storage	585:631	freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment	585:715	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	8	10	theme	cells	1283:1287	arg1	spectra					1258:1264	the FTIR spectra	1249:1264	the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage	1249:1340	The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage revealed that freeze-drying induced damage to proteins, peptidoglycans of the cell wall and nucleic acids.					
36192613	6	11	theme	transition	993:1002	arg1	temperature					1004:1014	Glass transition temperature	987:1014	Glass transition temperature of the liquid and freeze-dried bacterial suspensions	987:1067	Glass transition temperature of the liquid and freeze-dried bacterial suspensions and water activity of the freeze-dried samples were measured.					
36192613	14	12	theme	glassy	2271:2276	arg1	matrix					2278:2283	A glassy matrix	2269:2283	A glassy matrix containing maltodextrin	2269:2307	• A glassy matrix containing maltodextrin and an antioxidant ensured the highest storage stability.					
36192613	11	13	theme	glass	1942:1946	arg1	transition					1948:1957	the glass transition	1938:1957	a product temperature well below the glass transition temperature	1905:1969	Maintaining during the process a product temperature well below the glass transition temperature, especially during storage, appeared essential for L. salivarius CECT5713 storage stability.					
36192613	1	14	from	storage	288:294	arg1	state					309:313	the dried state	299:313	the dried state	299:313	Ligilactobacillus salivarius is a lactic acid bacterium exhibiting several health benefits but remains commercially underexploited due to its inability to survive during long-term storage in the dried state.					
36192613	8	15	theme	rehydrated	1293:1302	arg1	cells					1304:1308	rehydrated cells	1293:1308	rehydrated cells	1293:1308	The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage revealed that freeze-drying induced damage to proteins, peptidoglycans of the cell wall and nucleic acids.					
36192613	14	16	theme	highest	2340:2346	arg1	stability					2356:2364	the highest storage stability	2336:2364	the highest storage stability	2336:2364	• A glassy matrix containing maltodextrin and an antioxidant ensured the highest storage stability.					
36192613	1	17	theme	acid	149:152	arg1	bacterium					154:162	a lactic acid bacterium	140:162	a lactic acid bacterium exhibiting several health benefits	140:197	Ligilactobacillus salivarius is a lactic acid bacterium exhibiting several health benefits but remains commercially underexploited due to its inability to survive during long-term storage in the dried state.					
36192613	1	17	theme	acid	149:152	arg1	salivarius					126:135	Ligilactobacillus salivarius	108:135	Ligilactobacillus salivarius	108:135	Ligilactobacillus salivarius is a lactic acid bacterium exhibiting several health benefits but remains commercially underexploited due to its inability to survive during long-term storage in the dried state.					
36192613	6	18	theme	water	1073:1077	arg1	activity					1079:1086	water activity	1073:1086	water activity of the freeze-dried samples	1073:1114	Glass transition temperature of the liquid and freeze-dried bacterial suspensions and water activity of the freeze-dried samples were measured.					
36192613	10	19	theme	inactivation	1577:1588	arg1	rates					1590:1594	The inactivation rates	1573:1594	The inactivation rates of bacteria during storage	1573:1621	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	10	19	theme	inactivation	1577:1588	arg1	function					1642:1649	a function	1640:1649	a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature	1640:1785	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	5	20	theme	fatty	916:920	arg1	composition					927:937	The membrane fatty acid composition	903:937	The membrane fatty acid composition	903:937	The membrane fatty acid composition was determined after freeze-drying and storage.					
36192613	6	21	theme	suspensions	1057:1067	arg1	temperature					1004:1014	Glass transition temperature	987:1014	Glass transition temperature of the liquid and freeze-dried bacterial suspensions	987:1067	Glass transition temperature of the liquid and freeze-dried bacterial suspensions and water activity of the freeze-dried samples were measured.					
36192613	6	21	theme	suspensions	1057:1067	arg1	activity					1079:1086	water activity	1073:1086	water activity of the freeze-dried samples	1073:1114	Glass transition temperature of the liquid and freeze-dried bacterial suspensions and water activity of the freeze-dried samples were measured.					
36192613	2	22	theme	freeze-dried	497:508	arg1	stability					510:518	the freeze-dried stability	493:518	the freeze-dried stability of L. salivarius CECT5713	493:544	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	13	23	theme	cell	2217:2220	arg1	proteins					2222:2229	cell proteins	2217:2229	cell proteins	2217:2229	• Freeze-drying and storage mainly altered cell proteins, peptidoglycans, and nucleic acids.					
36192613	0	24	theme	Ligilactobacillus	69:85	arg1	CECT5713					98:105	freeze-dried Ligilactobacillus salivarius CECT5713	56:105	freeze-dried Ligilactobacillus salivarius CECT5713	56:105	Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713.					
36192613	1	25	theme	health	183:188	arg1	benefits					190:197	several health benefits	175:197	several health benefits	175:197	Ligilactobacillus salivarius is a lactic acid bacterium exhibiting several health benefits but remains commercially underexploited due to its inability to survive during long-term storage in the dried state.					
36192613	6	26	theme	freeze-dried	1034:1045	arg1	suspensions					1057:1067	the liquid and freeze-dried bacterial suspensions	1019:1067	the liquid and freeze-dried bacterial suspensions	1019:1067	Glass transition temperature of the liquid and freeze-dried bacterial suspensions and water activity of the freeze-dried samples were measured.					
36192613	0	27	from	Effect	0:5	arg1	stability					43:51	the storage stability	31:51	the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713	31:105	Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713.					
36192613	2	28	dep	molecules	376:384	arg1	antioxidants					412:423	antioxidants	412:423	antioxidants	412:423	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	2	28	dep	molecules	376:384	arg1	efficient					461:469	efficient	461:469	efficient	461:469	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	2	28	dep	molecules	376:384	arg1	molecules					376:384	various protective molecules	357:384	various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides)	357:452	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	2	28	dep	molecules	376:384	arg1	maltodextrin					387:398	maltodextrin	387:398	maltodextrin	387:398	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	2	28	dep	molecules	376:384	arg1	fructooligosaccharides					430:451	fructooligosaccharides	430:451	fructooligosaccharides	430:451	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	2	28	dep	molecules	376:384	arg1	trehalose					401:409	trehalose	401:409	trehalose	401:409	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	0	29	theme	CECT5713	98:105	arg1	stability					43:51	the storage stability	31:51	the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713	31:105	Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713.					
36192613	4	30	from	literature	879:888	arg1	bacteria					893:900	bacteria	893:900	bacteria	893:900	The assignment of principal absorption bands to cellular components was performed using data from the literature on bacteria.					
36192613	4	30	from	literature	879:888	arg1	data					865:868	data	865:868	data from the literature on bacteria	865:900	The assignment of principal absorption bands to cellular components was performed using data from the literature on bacteria.					
36192613	6	31	theme	liquid	1023:1028	arg1	suspensions					1057:1067	the liquid and freeze-dried bacterial suspensions	1019:1067	the liquid and freeze-dried bacterial suspensions	1019:1067	Glass transition temperature of the liquid and freeze-dried bacterial suspensions and water activity of the freeze-dried samples were measured.					
36192613	2	32	theme	salivarius	526:535	arg1	CECT5713					537:544	L. salivarius CECT5713	523:544	L. salivarius CECT5713	523:544	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	4	33	theme	principal	795:803	arg1	bands					816:820	principal absorption bands	795:820	principal absorption bands	795:820	The assignment of principal absorption bands to cellular components was performed using data from the literature on bacteria.					
36192613	3	34	from	37 °C	636:640	arg1	environment					705:715	an aqueous environment	694:715	an aqueous environment	694:715	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	3	34	from	37 °C	636:640	arg1	storage					625:631	freezing, freeze-drying, and subsequent storage	585:631	freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment	585:715	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	11	35	theme	salivarius	2025:2034	arg1	stability					2053:2061	L. salivarius CECT5713 storage stability	2022:2061	L. salivarius CECT5713 storage stability	2022:2061	Maintaining during the process a product temperature well below the glass transition temperature, especially during storage, appeared essential for L. salivarius CECT5713 storage stability.					
36192613	1	36	theme	dried	303:307	arg1	state					309:313	the dried state	299:313	the dried state	299:313	Ligilactobacillus salivarius is a lactic acid bacterium exhibiting several health benefits but remains commercially underexploited due to its inability to survive during long-term storage in the dried state.					
36192613	0	37	theme	protective	10:19	arg1	agents					21:26	protective agents	10:26	protective agents	10:26	Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713.					
36192613	6	38	theme	freeze-dried	1095:1106	arg1	samples					1108:1114	the freeze-dried samples	1091:1114	the freeze-dried samples	1091:1114	Glass transition temperature of the liquid and freeze-dried bacterial suspensions and water activity of the freeze-dried samples were measured.					
36192613	3	39	theme	biochemical	658:668	arg1	composition					670:680	the biochemical composition	654:680	the biochemical composition of cells in an aqueous environment	654:715	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	11	40	theme	storage	2045:2051	arg1	stability					2053:2061	L. salivarius CECT5713 storage stability	2022:2061	L. salivarius CECT5713 storage stability	2022:2061	Maintaining during the process a product temperature well below the glass transition temperature, especially during storage, appeared essential for L. salivarius CECT5713 storage stability.					
36192613	3	41	dep	Fourier	723:729	arg1	transform					731:739	transform	731:739	transform infrared (FTIR) micro-spectroscopy	731:774	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	2	42	theme	various	357:363	arg1	molecules					376:384	various protective molecules	357:384	various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides)	357:452	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	2	42	theme	various	357:363	arg1	trehalose					401:409	trehalose	401:409	trehalose	401:409	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	2	42	theme	various	357:363	arg1	maltodextrin					387:398	maltodextrin	387:398	maltodextrin	387:398	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	0	43	theme	storage	35:41	arg1	stability					43:51	the storage stability	31:51	the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713	31:105	Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713.					
36192613	9	44	theme	Storage	1449:1455	arg1	stability					1457:1465	Storage stability	1449:1465	Storage stability	1449:1465	Storage stability appeared to be dependent on the ability of the protective molecules to limit damage during freeze-drying.					
36192613	14	45	contain	containing	2285:2294	arg2	maltodextrin					2296:2307	maltodextrin	2296:2307	maltodextrin	2296:2307	• A glassy matrix containing maltodextrin and an antioxidant ensured the highest storage stability.					
36192613	14	45	contain	containing	2285:2294	arg1	matrix					2278:2283	A glassy matrix	2269:2283	A glassy matrix containing maltodextrin	2269:2307	• A glassy matrix containing maltodextrin and an antioxidant ensured the highest storage stability.					
36192613	10	46	theme	bacteria	1599:1606	arg1	rates					1590:1594	The inactivation rates	1573:1594	The inactivation rates of bacteria during storage	1573:1621	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	10	46	theme	bacteria	1599:1606	arg1	function					1642:1649	a function	1640:1649	a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature	1640:1785	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	4	47	theme	bands	816:820	arg1	assignment					781:790	The assignment	777:790	The assignment of principal absorption bands to cellular components	777:843	The assignment of principal absorption bands to cellular components was performed using data from the literature on bacteria.					
36192613	3	48	from	composition	670:680	arg1	environment					705:715	an aqueous environment	694:715	an aqueous environment	694:715	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	3	48	from	composition	670:680	arg1	storage					625:631	freezing, freeze-drying, and subsequent storage	585:631	freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment	585:715	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	4	49	theme	cellular	825:832	arg1	components					834:843	cellular components	825:843	cellular components	825:843	The assignment of principal absorption bands to cellular components was performed using data from the literature on bacteria.					
36192613	3	50	from	cells	685:689	arg1	environment					705:715	an aqueous environment	694:715	an aqueous environment	694:715	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	2	51	theme	molecules	376:384	arg1	effect					347:352	the effect	343:352	the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713	343:544	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	1	52	theme	long-term	278:286	arg1	storage					288:294	long-term storage	278:294	long-term storage in the dried state	278:313	Ligilactobacillus salivarius is a lactic acid bacterium exhibiting several health benefits but remains commercially underexploited due to its inability to survive during long-term storage in the dried state.					
36192613	2	53	from	effect	347:352	arg1	stability					510:518	the freeze-dried stability	493:518	the freeze-dried stability of L. salivarius CECT5713	493:544	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	10	54	theme	temperature	1658:1668	arg1	difference					1670:1679	the temperature difference	1654:1679	the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature	1654:1785	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	3	55	theme	aqueous	697:703	arg1	environment					705:715	an aqueous environment	694:715	an aqueous environment	694:715	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	5	56	theme	acid	922:925	arg1	composition					927:937	The membrane fatty acid composition	903:937	The membrane fatty acid composition	903:937	The membrane fatty acid composition was determined after freeze-drying and storage.					
36192613	10	57	theme	freeze-dried	1851:1862	arg1	bacteria					1864:1871	freeze-dried bacteria	1851:1871	freeze-dried bacteria	1851:1871	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	10	58	theme	transition	1764:1773	arg1	temperature					1775:1785	the glass transition temperature	1754:1785	the glass transition temperature	1754:1785	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	8	59	theme	cells	1304:1308	arg1	spectra					1258:1264	the FTIR spectra	1249:1264	the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage	1249:1340	The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage revealed that freeze-drying induced damage to proteins, peptidoglycans of the cell wall and nucleic acids.					
36192613	10	60	theme	product	1693:1699	arg1	temperature					1701:1711	the product temperature	1689:1711	the product temperature during sublimation or during storage and the glass transition temperature	1689:1785	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	7	61	theme	best	1135:1138	arg1	stability					1148:1156	The best storage stability	1131:1156	The best storage stability	1131:1156	The best storage stability was observed for the formulations involving maltodextrin and antioxidants.					
36192613	9	62	theme	molecules	1525:1533	arg1	ability					1499:1505	the ability	1495:1505	the ability of the protective molecules to limit damage during freeze-drying	1495:1570	Storage stability appeared to be dependent on the ability of the protective molecules to limit damage during freeze-drying.					
36192613	12	63	theme	salivarius	2081:2090	arg1	CECT5713					2092:2099	• L. salivarius CECT5713	2076:2099	• L. salivarius CECT5713	2076:2099	KEY POINTS: • L. salivarius CECT5713 highly resisted freezing but was sensitive to freeze-drying and storage.					
36192613	8	64	theme	spectra	1258:1264	arg1	analysis					1237:1244	The analysis	1233:1244	The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage	1233:1340	The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage revealed that freeze-drying induced damage to proteins, peptidoglycans of the cell wall and nucleic acids.					
36192613	11	65	theme	product	1907:1913	arg1	temperature					1915:1925	a product temperature	1905:1925	a product temperature well below the glass transition temperature	1905:1969	Maintaining during the process a product temperature well below the glass transition temperature, especially during storage, appeared essential for L. salivarius CECT5713 storage stability.					
36192613	13	66	theme	nucleic	2252:2258	arg1	acids					2260:2264	nucleic acids	2252:2264	nucleic acids	2252:2264	• Freeze-drying and storage mainly altered cell proteins, peptidoglycans, and nucleic acids.					
36192613	3	67	theme	subsequent	614:623	arg1	storage					625:631	freezing, freeze-drying, and subsequent storage	585:631	freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment	585:715	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	8	68	theme	freeze-thawed	1269:1281	arg1	cells					1283:1287	freeze-thawed cells	1269:1287	freeze-thawed cells	1269:1287	The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage revealed that freeze-drying induced damage to proteins, peptidoglycans of the cell wall and nucleic acids.					
36192613	6	69	theme	Glass	987:991	arg1	temperature					1004:1014	Glass transition temperature	987:1014	Glass transition temperature of the liquid and freeze-dried bacterial suspensions	987:1067	Glass transition temperature of the liquid and freeze-dried bacterial suspensions and water activity of the freeze-dried samples were measured.					
36192613	3	70	from	environment	705:715	arg1	37 °C					636:640	37 °C	636:640	37 °C	636:640	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	3	70	from	environment	705:715	arg1	composition					670:680	the biochemical composition	654:680	the biochemical composition of cells in an aqueous environment	654:715	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	11	71	theme	transition	1948:1957	arg1	temperature					1959:1969	the glass transition temperature	1938:1969	a product temperature well below the glass transition temperature	1905:1969	Maintaining during the process a product temperature well below the glass transition temperature, especially during storage, appeared essential for L. salivarius CECT5713 storage stability.					
36192613	12	72	theme	•	2076:2076	arg1	CECT5713					2092:2099	• L. salivarius CECT5713	2076:2099	• L. salivarius CECT5713	2076:2099	KEY POINTS: • L. salivarius CECT5713 highly resisted freezing but was sensitive to freeze-drying and storage.					
36192613	1	73	theme	lactic	142:147	arg1	bacterium					154:162	a lactic acid bacterium	140:162	a lactic acid bacterium exhibiting several health benefits	140:197	Ligilactobacillus salivarius is a lactic acid bacterium exhibiting several health benefits but remains commercially underexploited due to its inability to survive during long-term storage in the dried state.					
36192613	1	73	theme	lactic	142:147	arg1	salivarius					126:135	Ligilactobacillus salivarius	108:135	Ligilactobacillus salivarius	108:135	Ligilactobacillus salivarius is a lactic acid bacterium exhibiting several health benefits but remains commercially underexploited due to its inability to survive during long-term storage in the dried state.					
36192613	8	74	theme	wall	1425:1428	arg1	peptidoglycans					1398:1411	peptidoglycans	1398:1411	peptidoglycans of the cell wall	1398:1428	The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage revealed that freeze-drying induced damage to proteins, peptidoglycans of the cell wall and nucleic acids.					
36192613	8	74	theme	wall	1425:1428	arg1	proteins					1388:1395	proteins	1388:1395	proteins	1388:1395	The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage revealed that freeze-drying induced damage to proteins, peptidoglycans of the cell wall and nucleic acids.					
36192613	8	74	theme	wall	1425:1428	arg1	acids					1442:1446	nucleic acids	1434:1446	nucleic acids	1434:1446	The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage revealed that freeze-drying induced damage to proteins, peptidoglycans of the cell wall and nucleic acids.					
36192613	0	75	theme	freeze-dried	56:67	arg1	CECT5713					98:105	freeze-dried Ligilactobacillus salivarius CECT5713	56:105	freeze-dried Ligilactobacillus salivarius CECT5713	56:105	Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713.					
36192613	5	76	theme	membrane	907:914	arg1	composition					927:937	The membrane fatty acid composition	903:937	The membrane fatty acid composition	903:937	The membrane fatty acid composition was determined after freeze-drying and storage.					
36192613	0	77	theme	salivarius	87:96	arg1	CECT5713					98:105	freeze-dried Ligilactobacillus salivarius CECT5713	56:105	freeze-dried Ligilactobacillus salivarius CECT5713	56:105	Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713.					
36192613	1	78	theme	several	175:181	arg1	benefits					190:197	several health benefits	175:197	several health benefits	175:197	Ligilactobacillus salivarius is a lactic acid bacterium exhibiting several health benefits but remains commercially underexploited due to its inability to survive during long-term storage in the dried state.					
36192613	6	79	theme	bacterial	1047:1055	arg1	suspensions					1057:1067	the liquid and freeze-dried bacterial suspensions	1019:1067	the liquid and freeze-dried bacterial suspensions	1019:1067	Glass transition temperature of the liquid and freeze-dried bacterial suspensions and water activity of the freeze-dried samples were measured.					
36192613	11	80	dep	well	1927:1930	arg1	below					1932:1936	below	1932:1936	below	1932:1936	Maintaining during the process a product temperature well below the glass transition temperature, especially during storage, appeared essential for L. salivarius CECT5713 storage stability.					
36192613	12	81	theme	KEY	2064:2066	arg1	POINTS					2068:2073	KEY POINTS	2064:2073	KEY POINTS: • L. salivarius CECT5713 highly resisted freezing but was sensitive to freeze-drying and storage.	2064:2172	KEY POINTS: • L. salivarius CECT5713 highly resisted freezing but was sensitive to freeze-drying and storage.					
36192613	2	82	theme	CECT5713	537:544	arg1	stability					510:518	the freeze-dried stability	493:518	the freeze-dried stability of L. salivarius CECT5713	493:544	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	12	83	dep	POINTS	2068:2073	arg1	sensitive					2134:2142	sensitive	2134:2142	sensitive	2134:2142	KEY POINTS: • L. salivarius CECT5713 highly resisted freezing but was sensitive to freeze-drying and storage.					
36192613	12	83	dep	POINTS	2068:2073	arg1	resisted					2108:2115	resisted	2108:2115	highly resisted freezing	2101:2124	KEY POINTS: • L. salivarius CECT5713 highly resisted freezing but was sensitive to freeze-drying and storage.					
36192613	8	84	theme	cell	1420:1423	arg1	wall					1425:1428	the cell wall	1416:1428	the cell wall	1416:1428	The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage revealed that freeze-drying induced damage to proteins, peptidoglycans of the cell wall and nucleic acids.					
36192613	13	85	theme	•	2174:2174	arg1	Freeze-drying					2176:2188	• Freeze-drying	2174:2188	• Freeze-drying	2174:2188	• Freeze-drying and storage mainly altered cell proteins, peptidoglycans, and nucleic acids.					
36192613	2	86	theme	L.	523:524	arg1	CECT5713					537:544	L. salivarius CECT5713	523:544	L. salivarius CECT5713	523:544	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	11	87	theme	CECT5713	2036:2043	arg1	stability					2053:2061	L. salivarius CECT5713 storage stability	2022:2061	L. salivarius CECT5713 storage stability	2022:2061	Maintaining during the process a product temperature well below the glass transition temperature, especially during storage, appeared essential for L. salivarius CECT5713 storage stability.					
36192613	3	88	dep	infrared	741:748	arg1	FTIR					751:754	FTIR	751:754	FTIR	751:754	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	11	89	theme	L.	2022:2023	arg1	stability					2053:2061	L. salivarius CECT5713 storage stability	2022:2061	L. salivarius CECT5713 storage stability	2022:2061	Maintaining during the process a product temperature well below the glass transition temperature, especially during storage, appeared essential for L. salivarius CECT5713 storage stability.					
36192613	0	90	theme	agents	21:26	arg1	Effect					0:5	Effect	0:5	Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713.	0:106	Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713.					
36192613	11	91	dep	appeared	1999:2006	arg1	Maintaining					1874:1884	Maintaining	1874:1884	Maintaining	1874:1884	Maintaining during the process a product temperature well below the glass transition temperature, especially during storage, appeared essential for L. salivarius CECT5713 storage stability.					
36192613	6	92	theme	samples	1108:1114	arg1	temperature					1004:1014	Glass transition temperature	987:1014	Glass transition temperature of the liquid and freeze-dried bacterial suspensions	987:1067	Glass transition temperature of the liquid and freeze-dried bacterial suspensions and water activity of the freeze-dried samples were measured.					
36192613	6	92	theme	samples	1108:1114	arg1	activity					1079:1086	water activity	1073:1086	water activity of the freeze-dried samples	1073:1114	Glass transition temperature of the liquid and freeze-dried bacterial suspensions and water activity of the freeze-dried samples were measured.					
36192613	8	93	theme	nucleic	1434:1440	arg1	acids					1442:1446	nucleic acids	1434:1446	nucleic acids	1434:1446	The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage revealed that freeze-drying induced damage to proteins, peptidoglycans of the cell wall and nucleic acids.					
36192613	10	94	theme	glass	1758:1762	arg1	transition					1764:1773	the glass transition	1754:1773	the glass transition temperature	1754:1785	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	2	95	theme	other	474:478	arg1	bacteria					480:487	other bacteria	474:487	other bacteria	474:487	Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713.					
36192613	3	96	theme	cells	685:689	arg1	37 °C					636:640	37 °C	636:640	37 °C	636:640	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	3	96	theme	cells	685:689	arg1	composition					670:680	the biochemical composition	654:680	the biochemical composition of cells in an aqueous environment	654:715	The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier transform infrared (FTIR) micro-spectroscopy.					
36192613	10	97	theme	better	1799:1804	arg1	insight					1806:1812	a better insight	1797:1812	a better insight into the stabilization mechanisms of freeze-dried bacteria	1797:1871	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
36192613	4	98	theme	absorption	805:814	arg1	bands					816:820	principal absorption bands	795:820	principal absorption bands	795:820	The assignment of principal absorption bands to cellular components was performed using data from the literature on bacteria.					
36192613	12	99	theme	L.	2078:2079	arg1	CECT5713					2092:2099	• L. salivarius CECT5713	2076:2099	• L. salivarius CECT5713	2076:2099	KEY POINTS: • L. salivarius CECT5713 highly resisted freezing but was sensitive to freeze-drying and storage.					
36192613	10	100	theme	stabilization	1823:1835	arg1	mechanisms					1837:1846	the stabilization mechanisms	1819:1846	the stabilization mechanisms of freeze-dried bacteria	1819:1871	The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria.					
37158281	5	0	theme	toxic	617:621	arg1	compounds					635:643	highly toxic chlorinated compounds	610:643	highly toxic chlorinated compounds	610:643	However, recent studies have shown the potential formation of highly toxic chlorinated compounds.					
37158281	10	1	theme	tandem	1355:1360	arg1	spectrometer					1367:1378	a tandem mass spectrometer	1353:1378	a tandem mass spectrometer method	1353:1385	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
37158281	5	2	theme	compounds	635:643	arg1	formation					597:605	the potential formation	583:605	the potential formation of highly toxic chlorinated compounds	583:643	However, recent studies have shown the potential formation of highly toxic chlorinated compounds.					
37158281	6	3	theme	basic	746:750	arg1	composition					752:762	the used basic composition	737:762	the used basic composition	737:762	This can be explained by the high temperatures (above 120 °C) within the heating coils and the used basic composition of these liquids.					
37158281	7	4	theme	legal	800:804	arg1	situation					806:814	the legal situation	796:814	the legal situation	796:814	Nevertheless, the legal situation is composed of proposals without clear restrictions, only recommendations for tobacco products.					
37158281	5	5	theme	potential	587:595	arg1	formation					597:605	the potential formation	583:605	the potential formation of highly toxic chlorinated compounds	583:643	However, recent studies have shown the potential formation of highly toxic chlorinated compounds.					
37158281	9	6	theme	e-liquids	1128:1136	arg1	number					1091:1096	a number	1089:1096	a number of 100 commercially available e-liquids	1089:1136	In this study, a number of 100 commercially available e-liquids was screened for sucralose in order to identify the suitability of ambient mass spectrometry and near-infrared spectroscopy for this application.					
37158281	12	7	theme	products	1681:1688	arg1	products					1681:1688	the used products	1672:1688	the used products	1672:1688	The results clearly revile the necessity for product quality due to the absence of declaration on many of the used products.					
37158281	12	7	theme	products	1681:1688	arg1	many					1664:1667	many	1664:1667	many	1664:1667	The results clearly revile the necessity for product quality due to the absence of declaration on many of the used products.					
37158281	10	8	theme	spectrometer	1367:1378	arg1	method					1380:1385	a tandem mass spectrometer method	1353:1385	a tandem mass spectrometer method	1353:1385	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
37158281	15	9	theme	labelling	2142:2150	arg1	elimination					2111:2121	elimination	2111:2121	elimination of confuse package labelling	2111:2150	In summary, these methods enable an important contribution to ensure consumer protection and elimination of confuse package labelling.					
37158281	15	9	theme	labelling	2142:2150	arg1	protection					2096:2105	consumer protection	2087:2105	consumer protection	2087:2105	In summary, these methods enable an important contribution to ensure consumer protection and elimination of confuse package labelling.					
37158281	8	10	theme	interest	945:952	arg1	level					936:940	a high level	929:940	a high level of interest	929:952	For this reason, a high level of interest lies within the establishment of fast, reliable and cost-effective methods for the detection of sucralose in e-liquids.					
37158281	13	11	theme	liquid	1911:1916	arg1	chromatography					1918:1931	high-performance liquid chromatography	1894:1931	high-performance liquid chromatography	1894:1931	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	11	12	theme	methods	1482:1488	arg1	limitations					1449:1459	limitations	1449:1459	limitations	1449:1459	Furthermore, the advantages and limitations of the two mentioned methods are highlighted in order to provide a reliable quantification of sucralose.					
37158281	11	12	theme	methods	1482:1488	arg1	advantages					1434:1443	advantages	1434:1443	advantages	1434:1443	Furthermore, the advantages and limitations of the two mentioned methods are highlighted in order to provide a reliable quantification of sucralose.					
37158281	0	13	theme	sucralose	116:124	arg1	quantification					98:111	the quantification	94:111	the quantification of sucralose in e-liquids	94:137	Ambient mass spectrometry and near-infrared spectroscopy - a direct comparison of methods for the quantification of sucralose in e-liquids.					
37158281	2	14	theme	wide	213:216	arg1	flavors					229:235	flavors	229:235	flavors	229:235	A wide variety of flavors and nicotine strengths make it possible for every user to get a product according to their wishes.					
37158281	2	14	theme	wide	213:216	arg1	strengths					250:258	nicotine strengths	241:258	nicotine strengths	241:258	A wide variety of flavors and nicotine strengths make it possible for every user to get a product according to their wishes.					
37158281	2	14	theme	wide	213:216	arg1	variety					218:224	A wide variety	211:224	A wide variety of flavors and nicotine strengths	211:258	A wide variety of flavors and nicotine strengths make it possible for every user to get a product according to their wishes.					
37158281	0	15	from	quantification	98:111	arg1	e-liquids					129:137	e-liquids	129:137	e-liquids	129:137	Ambient mass spectrometry and near-infrared spectroscopy - a direct comparison of methods for the quantification of sucralose in e-liquids.					
37158281	2	16	theme	strengths	250:258	arg1	flavors					229:235	flavors	229:235	flavors	229:235	A wide variety of flavors and nicotine strengths make it possible for every user to get a product according to their wishes.					
37158281	2	16	theme	strengths	250:258	arg1	strengths					250:258	nicotine strengths	241:258	nicotine strengths	241:258	A wide variety of flavors and nicotine strengths make it possible for every user to get a product according to their wishes.					
37158281	2	16	theme	strengths	250:258	arg1	variety					218:224	A wide variety	211:224	A wide variety of flavors and nicotine strengths	211:258	A wide variety of flavors and nicotine strengths make it possible for every user to get a product according to their wishes.					
37158281	1	17	from	society	186:192	arg1	popular					175:181	popular	175:181	popular	175:181	E-liquids have become increasingly popular in society in recent years.					
37158281	12	18	theme	used	1676:1679	arg1	products					1681:1688	the used products	1672:1688	the used products	1672:1688	The results clearly revile the necessity for product quality due to the absence of declaration on many of the used products.					
37158281	13	19	theme	classical	1857:1865	arg1	chromatography					1918:1931	high-performance liquid chromatography	1894:1931	high-performance liquid chromatography	1894:1931	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	13	19	theme	classical	1857:1865	arg1	tools					1878:1882	classical analytical tools	1857:1882	classical analytical tools including high-performance liquid chromatography	1857:1931	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	0	20	theme	direct	61:66	arg1	comparison					68:77	a direct comparison	59:77	Ambient mass spectrometry and near-infrared spectroscopy - a direct comparison of methods for the quantification of sucralose in e-liquids.	0:138	Ambient mass spectrometry and near-infrared spectroscopy - a direct comparison of methods for the quantification of sucralose in e-liquids.					
37158281	9	21	theme	ambient	1205:1211	arg1	spectrometry					1218:1229	ambient mass spectrometry	1205:1229	ambient mass spectrometry	1205:1229	In this study, a number of 100 commercially available e-liquids was screened for sucralose in order to identify the suitability of ambient mass spectrometry and near-infrared spectroscopy for this application.					
37158281	1	22	from	popular	175:181	arg1	society					186:192	society	186:192	society	186:192	E-liquids have become increasingly popular in society in recent years.					
37158281	13	23	theme	economic	1819:1826	arg1	aspects					1843:1849	beneficial economic and ecological aspects	1808:1849	beneficial economic and ecological aspects	1808:1849	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	9	24	theme	spectrometry	1218:1229	arg1	suitability					1190:1200	the suitability	1186:1200	the suitability of ambient mass spectrometry and near-infrared spectroscopy for this application	1186:1281	In this study, a number of 100 commercially available e-liquids was screened for sucralose in order to identify the suitability of ambient mass spectrometry and near-infrared spectroscopy for this application.					
37158281	5	25	theme	recent	557:562	arg1	studies					564:570	recent studies	557:570	recent studies	557:570	However, recent studies have shown the potential formation of highly toxic chlorinated compounds.					
37158281	10	26	used	used	1391:1394	arg2	chromatography					1327:1340	A highly sensitive high-performance liquid chromatography	1284:1340	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method	1284:1385	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
37158281	10	26	used	used	1391:1394	arg2	method					1409:1414	reference method	1399:1414	reference method	1399:1414	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
37158281	13	27	theme	ecological	1832:1841	arg1	aspects					1843:1849	beneficial economic and ecological aspects	1808:1849	beneficial economic and ecological aspects	1808:1849	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	9	28	theme	near-infrared	1235:1247	arg1	spectroscopy					1249:1260	near-infrared spectroscopy	1235:1260	near-infrared spectroscopy	1235:1260	In this study, a number of 100 commercially available e-liquids was screened for sucralose in order to identify the suitability of ambient mass spectrometry and near-infrared spectroscopy for this application.					
37158281	14	29	theme	developed	1985:1993	arg1	methods					1995:2001	the reference and novel developed methods	1961:2001	methods	1995:2001	Clear correlations between the reference and novel developed methods are displayed.					
37158281	8	30	from	detection	1037:1045	arg1	e-liquids					1063:1071	e-liquids	1063:1071	e-liquids	1063:1071	For this reason, a high level of interest lies within the establishment of fast, reliable and cost-effective methods for the detection of sucralose in e-liquids.					
37158281	8	31	theme	fast	987:990	arg1	methods					1021:1027	fast, reliable and cost-effective methods	987:1027	fast, reliable and cost-effective methods for the detection of sucralose in e-liquids	987:1071	For this reason, a high level of interest lies within the establishment of fast, reliable and cost-effective methods for the detection of sucralose in e-liquids.					
37158281	0	32	theme	Ambient	0:6	arg1	spectrometry					13:24	Ambient mass spectrometry	0:24	Ambient mass spectrometry	0:24	Ambient mass spectrometry and near-infrared spectroscopy - a direct comparison of methods for the quantification of sucralose in e-liquids.					
37158281	8	33	theme	reliable	993:1000	arg1	methods					1021:1027	fast, reliable and cost-effective methods	987:1027	fast, reliable and cost-effective methods for the detection of sucralose in e-liquids	987:1071	For this reason, a high level of interest lies within the establishment of fast, reliable and cost-effective methods for the detection of sucralose in e-liquids.					
37158281	7	34	theme	only	869:872	arg1	restrictions					855:866	clear restrictions	849:866	clear restrictions	849:866	Nevertheless, the legal situation is composed of proposals without clear restrictions, only recommendations for tobacco products.					
37158281	7	34	theme	only	869:872	arg1	recommendations					874:888	only recommendations	869:888	only recommendations for tobacco products	869:909	Nevertheless, the legal situation is composed of proposals without clear restrictions, only recommendations for tobacco products.					
37158281	9	35	theme	available	1118:1126	arg1	e-liquids					1128:1136	100 commercially available e-liquids	1101:1136	100 commercially available e-liquids	1101:1136	In this study, a number of 100 commercially available e-liquids was screened for sucralose in order to identify the suitability of ambient mass spectrometry and near-infrared spectroscopy for this application.					
37158281	14	36	theme	Clear	1934:1938	arg1	correlations					1940:1951	Clear correlations	1934:1951	Clear correlations between the reference and novel developed methods	1934:2001	Clear correlations between the reference and novel developed methods are displayed.					
37158281	8	37	theme	cost-effective	1006:1019	arg1	methods					1021:1027	fast, reliable and cost-effective methods	987:1027	fast, reliable and cost-effective methods for the detection of sucralose in e-liquids	987:1071	For this reason, a high level of interest lies within the establishment of fast, reliable and cost-effective methods for the detection of sucralose in e-liquids.					
37158281	12	38	theme	product	1611:1617	arg1	quality					1619:1625	product quality	1611:1625	product quality	1611:1625	The results clearly revile the necessity for product quality due to the absence of declaration on many of the used products.					
37158281	3	39	theme	different	388:396	arg1	flavors					398:404	countless different flavors	378:404	countless different flavors	378:404	Many of these e-liquids are marketed with countless different flavors, which are often characterized by a strong and sweet smell.					
37158281	13	40	theme	sucralose	1779:1787	arg1	quantification					1761:1774	the quantification	1757:1774	the quantification of sucralose in e-liquids	1757:1800	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	0	41	theme	near-infrared	30:42	arg1	spectroscopy					44:55	near-infrared spectroscopy	30:55	near-infrared spectroscopy	30:55	Ambient mass spectrometry and near-infrared spectroscopy - a direct comparison of methods for the quantification of sucralose in e-liquids.					
37158281	13	42	from	sucralose	1779:1787	arg1	e-liquids					1792:1800	e-liquids	1792:1800	e-liquids	1792:1800	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	13	43	from	quantification	1761:1774	arg1	e-liquids					1792:1800	e-liquids	1792:1800	e-liquids	1792:1800	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	13	44	theme	analytical	1867:1876	arg1	chromatography					1918:1931	high-performance liquid chromatography	1894:1931	high-performance liquid chromatography	1894:1931	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	13	44	theme	analytical	1867:1876	arg1	tools					1878:1882	classical analytical tools	1857:1882	classical analytical tools including high-performance liquid chromatography	1857:1931	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	13	45	with	suitable	1744:1751	arg1	aspects					1843:1849	beneficial economic and ecological aspects	1808:1849	beneficial economic and ecological aspects	1808:1849	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	8	46	theme	sucralose	1050:1058	arg1	detection					1037:1045	the detection	1033:1045	the detection of sucralose in e-liquids	1033:1071	For this reason, a high level of interest lies within the establishment of fast, reliable and cost-effective methods for the detection of sucralose in e-liquids.					
37158281	6	47	theme	liquids	773:779	arg1	composition					752:762	the used basic composition	737:762	the used basic composition	737:762	This can be explained by the high temperatures (above 120 °C) within the heating coils and the used basic composition of these liquids.					
37158281	6	47	theme	liquids	773:779	arg1	coils					727:731	the heating coils	715:731	the heating coils	715:731	This can be explained by the high temperatures (above 120 °C) within the heating coils and the used basic composition of these liquids.					
37158281	0	48	dep	spectrometry	13:24	arg1	comparison					68:77	a direct comparison	59:77	Ambient mass spectrometry and near-infrared spectroscopy - a direct comparison of methods for the quantification of sucralose in e-liquids.	0:138	Ambient mass spectrometry and near-infrared spectroscopy - a direct comparison of methods for the quantification of sucralose in e-liquids.					
37158281	10	49	theme	liquid	1320:1325	arg1	method					1409:1414	reference method	1399:1414	reference method	1399:1414	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
37158281	10	49	theme	liquid	1320:1325	arg1	chromatography					1327:1340	A highly sensitive high-performance liquid chromatography	1284:1340	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method	1284:1385	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
37158281	7	50	theme	clear	849:853	arg1	restrictions					855:866	clear restrictions	849:866	clear restrictions	849:866	Nevertheless, the legal situation is composed of proposals without clear restrictions, only recommendations for tobacco products.					
37158281	7	50	theme	clear	849:853	arg1	recommendations					874:888	only recommendations	869:888	only recommendations for tobacco products	869:909	Nevertheless, the legal situation is composed of proposals without clear restrictions, only recommendations for tobacco products.					
37158281	13	51	from	e-liquids	1792:1800	arg1	quantification					1761:1774	the quantification	1757:1774	the quantification of sucralose in e-liquids	1757:1800	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	10	52	theme	sensitive	1293:1301	arg1	method					1409:1414	reference method	1399:1414	reference method	1399:1414	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
37158281	10	52	theme	sensitive	1293:1301	arg1	chromatography					1327:1340	A highly sensitive high-performance liquid chromatography	1284:1340	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method	1284:1385	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
37158281	5	53	theme	chlorinated	623:633	arg1	compounds					635:643	highly toxic chlorinated compounds	610:643	highly toxic chlorinated compounds	610:643	However, recent studies have shown the potential formation of highly toxic chlorinated compounds.					
37158281	8	54	theme	high	931:934	arg1	level					936:940	a high level	929:940	a high level of interest	929:952	For this reason, a high level of interest lies within the establishment of fast, reliable and cost-effective methods for the detection of sucralose in e-liquids.					
37158281	6	55	theme	used	741:744	arg1	composition					752:762	the used basic composition	737:762	the used basic composition	737:762	This can be explained by the high temperatures (above 120 °C) within the heating coils and the used basic composition of these liquids.					
37158281	11	56	theme	reliable	1528:1535	arg1	quantification					1537:1550	a reliable quantification	1526:1550	a reliable quantification of sucralose	1526:1563	Furthermore, the advantages and limitations of the two mentioned methods are highlighted in order to provide a reliable quantification of sucralose.					
37158281	15	57	theme	package	2134:2140	arg1	labelling					2142:2150	confuse package labelling	2126:2150	confuse package labelling	2126:2150	In summary, these methods enable an important contribution to ensure consumer protection and elimination of confuse package labelling.					
37158281	10	58	theme	mass	1362:1365	arg1	spectrometer					1367:1378	a tandem mass spectrometer	1353:1378	a tandem mass spectrometer method	1353:1385	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
37158281	1	59	theme	recent	197:202	arg1	years					204:208	recent years	197:208	recent years	197:208	E-liquids have become increasingly popular in society in recent years.					
37158281	13	60	theme	high-performance	1894:1909	arg1	chromatography					1918:1931	high-performance liquid chromatography	1894:1931	high-performance liquid chromatography	1894:1931	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	2	61	theme	flavors	229:235	arg1	flavors					229:235	flavors	229:235	flavors	229:235	A wide variety of flavors and nicotine strengths make it possible for every user to get a product according to their wishes.					
37158281	2	61	theme	flavors	229:235	arg1	strengths					250:258	nicotine strengths	241:258	nicotine strengths	241:258	A wide variety of flavors and nicotine strengths make it possible for every user to get a product according to their wishes.					
37158281	2	61	theme	flavors	229:235	arg1	variety					218:224	A wide variety	211:224	A wide variety of flavors and nicotine strengths	211:258	A wide variety of flavors and nicotine strengths make it possible for every user to get a product according to their wishes.					
37158281	15	62	dep	labelling	2142:2150	arg1	confuse					2126:2132	confuse	2126:2132	confuse	2126:2132	In summary, these methods enable an important contribution to ensure consumer protection and elimination of confuse package labelling.					
37158281	12	63	from	many	1664:1667	arg1	absence					1638:1644	the absence	1634:1644	the absence of declaration on many of the used products	1634:1688	The results clearly revile the necessity for product quality due to the absence of declaration on many of the used products.					
37158281	6	64	theme	heating	719:725	arg1	coils					727:731	the heating coils	715:731	the heating coils	715:731	This can be explained by the high temperatures (above 120 °C) within the heating coils and the used basic composition of these liquids.					
37158281	2	65	dep	get	295:297	arg1	possible					268:275	possible	268:275	possible	268:275	A wide variety of flavors and nicotine strengths make it possible for every user to get a product according to their wishes.					
37158281	2	66	theme	nicotine	241:248	arg1	strengths					250:258	nicotine strengths	241:258	nicotine strengths	241:258	A wide variety of flavors and nicotine strengths make it possible for every user to get a product according to their wishes.					
37158281	12	67	from	absence	1638:1644	arg1	products					1681:1688	the used products	1672:1688	the used products	1672:1688	The results clearly revile the necessity for product quality due to the absence of declaration on many of the used products.					
37158281	12	67	from	absence	1638:1644	arg1	many					1664:1667	many	1664:1667	many	1664:1667	The results clearly revile the necessity for product quality due to the absence of declaration on many of the used products.					
37158281	13	68	theme	beneficial	1808:1817	arg1	aspects					1843:1849	beneficial economic and ecological aspects	1808:1849	beneficial economic and ecological aspects	1808:1849	Further on, it could be shown, that both methods are suitable for the quantification of sucralose in e-liquids, with beneficial economic and ecological aspects, over classical analytical tools including high-performance liquid chromatography.					
37158281	0	69	theme	methods	82:88	arg1	comparison					68:77	a direct comparison	59:77	Ambient mass spectrometry and near-infrared spectroscopy - a direct comparison of methods for the quantification of sucralose in e-liquids.	0:138	Ambient mass spectrometry and near-infrared spectroscopy - a direct comparison of methods for the quantification of sucralose in e-liquids.					
37158281	9	70	theme	mass	1213:1216	arg1	spectrometry					1218:1229	ambient mass spectrometry	1205:1229	ambient mass spectrometry	1205:1229	In this study, a number of 100 commercially available e-liquids was screened for sucralose in order to identify the suitability of ambient mass spectrometry and near-infrared spectroscopy for this application.					
37158281	4	71	dep	sugar	529:533	arg1	substitutes					535:545	substitutes	535:545	substitutes	535:545	Sweeteners, such as sucralose, are therefore commonly added as sugar substitutes.					
37158281	10	72	theme	reference	1399:1407	arg1	method					1409:1414	reference method	1399:1414	reference method	1399:1414	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
37158281	10	72	theme	reference	1399:1407	arg1	chromatography					1327:1340	A highly sensitive high-performance liquid chromatography	1284:1340	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method	1284:1385	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
37158281	11	73	dep	advantages	1434:1443	arg1	the					1430:1432	the	1430:1432	the	1430:1432	Furthermore, the advantages and limitations of the two mentioned methods are highlighted in order to provide a reliable quantification of sucralose.					
37158281	12	74	theme	declaration	1649:1659	arg1	absence					1638:1644	the absence	1634:1644	the absence of declaration on many of the used products	1634:1688	The results clearly revile the necessity for product quality due to the absence of declaration on many of the used products.					
37158281	6	75	theme	high	675:678	arg1	temperatures					680:691	the high temperatures	671:691	the high temperatures (above 120 °C) within the heating coils and the used basic composition of these liquids	671:779	This can be explained by the high temperatures (above 120 °C) within the heating coils and the used basic composition of these liquids.					
37158281	6	75	theme	high	675:678	arg1	°C					704:705	above 120 °C	694:705	above 120 °C	694:705	This can be explained by the high temperatures (above 120 °C) within the heating coils and the used basic composition of these liquids.					
37158281	9	76	theme	spectroscopy	1249:1260	arg1	suitability					1190:1200	the suitability	1186:1200	the suitability of ambient mass spectrometry and near-infrared spectroscopy for this application	1186:1281	In this study, a number of 100 commercially available e-liquids was screened for sucralose in order to identify the suitability of ambient mass spectrometry and near-infrared spectroscopy for this application.					
37158281	14	77	theme	novel	1979:1983	arg1	methods					1995:2001	the reference and novel developed methods	1961:2001	methods	1995:2001	Clear correlations between the reference and novel developed methods are displayed.					
37158281	11	78	theme	mentioned	1472:1480	arg1	methods					1482:1488	the two mentioned methods	1464:1488	the two mentioned methods	1464:1488	Furthermore, the advantages and limitations of the two mentioned methods are highlighted in order to provide a reliable quantification of sucralose.					
37158281	0	79	theme	mass	8:11	arg1	spectrometry					13:24	Ambient mass spectrometry	0:24	Ambient mass spectrometry	0:24	Ambient mass spectrometry and near-infrared spectroscopy - a direct comparison of methods for the quantification of sucralose in e-liquids.					
37158281	7	80	theme	tobacco	894:900	arg1	products					902:909	tobacco products	894:909	tobacco products	894:909	Nevertheless, the legal situation is composed of proposals without clear restrictions, only recommendations for tobacco products.					
37158281	8	81	theme	methods	1021:1027	arg1	establishment					970:982	the establishment	966:982	the establishment of fast, reliable and cost-effective methods for the detection of sucralose in e-liquids	966:1071	For this reason, a high level of interest lies within the establishment of fast, reliable and cost-effective methods for the detection of sucralose in e-liquids.					
37158281	3	82	theme	e-liquids	350:358	arg1	Many					336:339	Many	336:339	Many	336:339	Many of these e-liquids are marketed with countless different flavors, which are often characterized by a strong and sweet smell.					
37158281	3	82	theme	e-liquids	350:358	arg1	e-liquids					350:358	these e-liquids	344:358	these e-liquids	344:358	Many of these e-liquids are marketed with countless different flavors, which are often characterized by a strong and sweet smell.					
37158281	3	83	theme	strong	442:447	arg1	smell					459:463	a strong and sweet smell	440:463	a strong and sweet smell	440:463	Many of these e-liquids are marketed with countless different flavors, which are often characterized by a strong and sweet smell.					
37158281	15	84	theme	consumer	2087:2094	arg1	protection					2096:2105	consumer protection	2087:2105	consumer protection	2087:2105	In summary, these methods enable an important contribution to ensure consumer protection and elimination of confuse package labelling.					
37158281	11	85	theme	sucralose	1555:1563	arg1	quantification					1537:1550	a reliable quantification	1526:1550	a reliable quantification of sucralose	1526:1563	Furthermore, the advantages and limitations of the two mentioned methods are highlighted in order to provide a reliable quantification of sucralose.					
37158281	3	86	theme	countless	378:386	arg1	flavors					398:404	countless different flavors	378:404	countless different flavors	378:404	Many of these e-liquids are marketed with countless different flavors, which are often characterized by a strong and sweet smell.					
37158281	15	87	theme	important	2054:2062	arg1	contribution					2064:2075	an important contribution	2051:2075	an important contribution to ensure consumer protection and elimination of confuse package labelling	2051:2150	In summary, these methods enable an important contribution to ensure consumer protection and elimination of confuse package labelling.					
37158281	3	88	theme	sweet	453:457	arg1	smell					459:463	a strong and sweet smell	440:463	a strong and sweet smell	440:463	Many of these e-liquids are marketed with countless different flavors, which are often characterized by a strong and sweet smell.					
37158281	10	89	theme	high-performance	1303:1318	arg1	method					1409:1414	reference method	1399:1414	reference method	1399:1414	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
37158281	10	89	theme	high-performance	1303:1318	arg1	chromatography					1327:1340	A highly sensitive high-performance liquid chromatography	1284:1340	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method	1284:1385	A highly sensitive high-performance liquid chromatography coupled to a tandem mass spectrometer method was used as reference method.					
36080450	9	0	theme	FTIR	1713:1716	arg1	analysis					1718:1725	FTIR analysis	1713:1725	FTIR analysis	1713:1725	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	1	1	theme	specific	290:297	arg1	properties					307:316	specific surface properties	290:316	specific surface properties	290:316	The creation of novel surface morphologies through thin-film patterning is important from a scientific and technological viewpoint in order to control specific surface properties.					
36080450	2	2	theme	pulsed-plasma	323:335	arg1	polymerization					337:350	The pulsed-plasma polymerization	319:350	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC),	319:445	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	6	3	from	interface	1299:1307	arg1	interactions					1264:1275	interactions	1264:1275	interactions at the film/substrate interface	1264:1307	The structures particularly formed on hydrophilic substrates and were not observed before on the more hydrophobic substrates, as they exist as a result of water penetration and interactions at the film/substrate interface.					
36080450	6	3	from	interface	1299:1307	arg1	result					1232:1237	a result	1230:1237	a result of water penetration and interactions at the film/substrate interface	1230:1307	The structures particularly formed on hydrophilic substrates and were not observed before on the more hydrophobic substrates, as they exist as a result of water penetration and interactions at the film/substrate interface.					
36080450	6	3	from	interface	1299:1307	arg1	penetration					1248:1258	water penetration	1242:1258	water penetration	1242:1258	The structures particularly formed on hydrophilic substrates and were not observed before on the more hydrophobic substrates, as they exist as a result of water penetration and interactions at the film/substrate interface.					
36080450	9	4	theme	enhanced	1739:1746	arg1	stability					1759:1767	enhanced hydrolytic stability	1739:1767	enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films	1739:1833	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	4	5	theme	plasma-polymerized	715:732	arg1	films					757:761	plasma-polymerized (MA+CNC) nanocomposite films	715:761	plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere	715:811	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	3	6	theme	plasma	578:583	arg1	films					585:589	deposited plasma films	568:589	deposited plasma films	568:589	Alternatively, the transformation of deposited plasma films into crystalline structures introduces unique and more stable morphologies.					
36080450	4	7	theme	monomer	903:909	arg1	composition					911:921	monomer composition	903:921	monomer composition (ratio MA/CNC)	903:936	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	4	7	theme	monomer	903:909	arg1	MA/CNC					930:935	ratio MA/CNC	924:935	ratio MA/CNC	924:935	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	4	8	theme	MA+CNC	735:740	arg1	films					757:761	plasma-polymerized (MA+CNC) nanocomposite films	715:761	plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere	715:811	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	2	9	theme	nanocomposite	360:372	arg1	anhydride					398:406	maleic anhydride	391:406	maleic anhydride (MA)	391:411	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	2	9	theme	nanocomposite	360:372	arg1	nanocrystals					427:438	cellulose nanocrystals	417:438	cellulose nanocrystals (CNC)	417:444	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	2	9	theme	nanocomposite	360:372	arg1	films					374:378	thin nanocomposite films	355:378	thin nanocomposite films	355:378	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	6	10	theme	hydrophobic	1189:1199	arg1	substrates					1201:1210	the more hydrophobic substrates	1180:1210	the more hydrophobic substrates	1180:1210	The structures particularly formed on hydrophilic substrates and were not observed before on the more hydrophobic substrates, as they exist as a result of water penetration and interactions at the film/substrate interface.					
36080450	1	11	theme	scientific	231:240	arg1	viewpoint					260:268	a scientific and technological viewpoint	229:268	a scientific and technological viewpoint in order to control specific surface properties	229:316	The creation of novel surface morphologies through thin-film patterning is important from a scientific and technological viewpoint in order to control specific surface properties.					
36080450	1	12	theme	technological	246:258	arg1	viewpoint					260:268	a scientific and technological viewpoint	229:268	a scientific and technological viewpoint in order to control specific surface properties	229:316	The creation of novel surface morphologies through thin-film patterning is important from a scientific and technological viewpoint in order to control specific surface properties.					
36080450	9	13	theme	nanocomposite	1782:1794	arg1	films					1796:1800	p(MA+CNC) nanocomposite films	1772:1800	p(MA+CNC) nanocomposite films relative to the pure p(MA) films	1772:1833	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	5	14	theme	nm	1083:1084	arg1	films					1065:1069	confined thin films	1051:1069	confined thin films of 30 to 50 nm	1051:1084	The progressive growth of crystalline structures with fractal dendrites was observed in confined thin films of 30 to 50 nm.					
36080450	9	15	theme	relative	1802:1809	arg1	films					1796:1800	p(MA+CNC) nanocomposite films	1772:1800	p(MA+CNC) nanocomposite films relative to the pure p(MA) films	1772:1833	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	6	16	theme	water	1242:1246	arg1	penetration					1248:1258	water penetration	1242:1258	water penetration	1242:1258	The structures particularly formed on hydrophilic substrates and were not observed before on the more hydrophobic substrates, as they exist as a result of water penetration and interactions at the film/substrate interface.					
36080450	1	17	theme	novel	155:159	arg1	morphologies					169:180	novel surface morphologies	155:180	novel surface morphologies	155:180	The creation of novel surface morphologies through thin-film patterning is important from a scientific and technological viewpoint in order to control specific surface properties.					
36080450	4	18	theme	nanocomposite	743:755	arg1	films					757:761	plasma-polymerized (MA+CNC) nanocomposite films	715:761	plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere	715:811	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	0	19	theme	Maleic	77:82	arg1	Anhydride					84:92	Maleic Anhydride	77:92	Maleic Anhydride	77:92	Confined Crystallization of Thin Plasma-Polymerized Nanocomposite Films with Maleic Anhydride and Cellulose Nanocrystals under Hydrolysis.					
36080450	1	20	theme	morphologies	169:180	arg1	important					214:222	important	214:222	important	214:222	The creation of novel surface morphologies through thin-film patterning is important from a scientific and technological viewpoint in order to control specific surface properties.					
36080450	1	20	theme	morphologies	169:180	arg1	creation					143:150	The creation	139:150	The creation of novel surface morphologies through thin-film patterning	139:209	The creation of novel surface morphologies through thin-film patterning is important from a scientific and technological viewpoint in order to control specific surface properties.					
36080450	9	21	theme	p	1823:1823	arg1	films					1829:1833	the pure p(MA) films	1814:1833	the pure p(MA) films	1814:1833	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	1	22	theme	thin-film	190:198	arg1	patterning					200:209	thin-film patterning	190:209	thin-film patterning	190:209	The creation of novel surface morphologies through thin-film patterning is important from a scientific and technological viewpoint in order to control specific surface properties.					
36080450	8	23	theme	carboxylic	1565:1574	arg1	groups					1581:1586	the MA carboxylic acid groups	1558:1586	the MA carboxylic acid groups	1558:1586	The chemical structures after hydrolysis were further examined through XPS, indicating esterification between the MA carboxylic acid groups and CNC surface.					
36080450	2	24	theme	film	482:485	arg1	morphologies					487:498	different metastable film morphologies	461:498	different metastable film morphologies that are difficult to control	461:528	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	8	25	theme	CNC	1592:1594	arg1	surface					1596:1602	CNC surface	1592:1602	CNC surface	1592:1602	The chemical structures after hydrolysis were further examined through XPS, indicating esterification between the MA carboxylic acid groups and CNC surface.					
36080450	5	26	theme	crystalline	989:999	arg1	structures					1001:1010	crystalline structures	989:1010	crystalline structures	989:1010	The progressive growth of crystalline structures with fractal dendrites was observed in confined thin films of 30 to 50 nm.					
36080450	2	27	theme	different	461:469	arg1	morphologies					487:498	different metastable film morphologies	461:498	different metastable film morphologies that are difficult to control	461:528	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	5	28	located	observed	1039:1046	arg2	growth					979:984	The progressive growth	963:984	The progressive growth of crystalline structures with fractal dendrites	963:1033	The progressive growth of crystalline structures with fractal dendrites was observed in confined thin films of 30 to 50 nm.					
36080450	5	28	located	observed	1039:1046	arg1	films					1065:1069	confined thin films	1051:1069	confined thin films of 30 to 50 nm	1051:1084	The progressive growth of crystalline structures with fractal dendrites was observed in confined thin films of 30 to 50 nm.					
36080450	0	29	theme	Plasma-Polymerized	33:50	arg1	Films					66:70	Thin Plasma-Polymerized Nanocomposite Films	28:70	Thin Plasma-Polymerized Nanocomposite Films	28:70	Confined Crystallization of Thin Plasma-Polymerized Nanocomposite Films with Maleic Anhydride and Cellulose Nanocrystals under Hydrolysis.					
36080450	8	30	theme	chemical	1452:1459	arg1	structures					1461:1470	The chemical structures	1448:1470	The chemical structures after hydrolysis	1448:1487	The chemical structures after hydrolysis were further examined through XPS, indicating esterification between the MA carboxylic acid groups and CNC surface.					
36080450	0	31	theme	Films	66:70	arg1	Crystallization					9:23	Crystallization	9:23	Crystallization of Thin Plasma-Polymerized Nanocomposite Films with Maleic Anhydride and Cellulose Nanocrystals	9:119	Confined Crystallization of Thin Plasma-Polymerized Nanocomposite Films with Maleic Anhydride and Cellulose Nanocrystals under Hydrolysis.					
36080450	9	32	theme	groups	1678:1683	arg1	conversion					1654:1663	the conversion	1650:1663	the conversion of anhydride groups into carboxylic moieties	1650:1708	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	7	33	theme	nucleating	1327:1336	arg1	effect					1338:1343	nucleating effect	1327:1343	nucleating effect	1327:1343	Furthermore, the nucleating effect and local pinning of the crystallites to the substrate near CNC positions enhanced the film stability.					
36080450	4	34	theme	conditions	855:864	arg1	effects					837:843	effects	837:843	effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability	837:960	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	5	35	theme	confined	1051:1058	arg1	films					1065:1069	confined thin films	1051:1069	confined thin films of 30 to 50 nm	1051:1084	The progressive growth of crystalline structures with fractal dendrites was observed in confined thin films of 30 to 50 nm.					
36080450	4	36	theme	low	867:869	arg1	cycle					876:880	low duty cycle	867:880	low duty cycle	867:880	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	4	36	theme	low	867:869	arg1	power					892:896	variable power	883:896	variable power	883:896	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	4	37	theme	composition	911:921	arg1	effects					837:843	effects	837:843	effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability	837:960	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	4	38	theme	variable	883:890	arg1	cycle					876:880	low duty cycle	867:880	low duty cycle	867:880	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	4	38	theme	variable	883:890	arg1	power					892:896	variable power	883:896	variable power	883:896	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	3	39	theme	films	585:589	arg1	transformation					550:563	the transformation	546:563	the transformation of deposited plasma films into crystalline structures	546:617	Alternatively, the transformation of deposited plasma films into crystalline structures introduces unique and more stable morphologies.					
36080450	1	40	theme	surface	299:305	arg1	properties					307:316	specific surface properties	290:316	specific surface properties	290:316	The creation of novel surface morphologies through thin-film patterning is important from a scientific and technological viewpoint in order to control specific surface properties.					
36080450	4	41	theme	ratio	924:928	arg1	composition					911:921	monomer composition	903:921	monomer composition (ratio MA/CNC)	903:936	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	4	41	theme	ratio	924:928	arg1	MA/CNC					930:935	ratio MA/CNC	924:935	ratio MA/CNC	924:935	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	3	42	theme	deposited	568:576	arg1	films					585:589	deposited plasma films	568:589	deposited plasma films	568:589	Alternatively, the transformation of deposited plasma films into crystalline structures introduces unique and more stable morphologies.					
36080450	9	43	theme	hydrolytic	1748:1757	arg1	stability					1759:1767	enhanced hydrolytic stability	1739:1767	enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films	1739:1833	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	5	44	dep	50	1080:1081	arg1	to					1077:1078	to	1077:1078	to	1077:1078	The progressive growth of crystalline structures with fractal dendrites was observed in confined thin films of 30 to 50 nm.					
36080450	6	45	theme	film/substrate	1284:1297	arg1	interface					1299:1307	the film/substrate interface	1280:1307	the film/substrate interface	1280:1307	The structures particularly formed on hydrophilic substrates and were not observed before on the more hydrophobic substrates, as they exist as a result of water penetration and interactions at the film/substrate interface.					
36080450	2	46	theme	films	374:378	arg1	polymerization					337:350	The pulsed-plasma polymerization	319:350	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC),	319:445	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	4	47	dep	conditions	855:864	arg1	cycle					876:880	low duty cycle	867:880	low duty cycle	867:880	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	4	47	dep	conditions	855:864	arg1	power					892:896	variable power	883:896	variable power	883:896	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	2	48	theme	thin	355:358	arg1	anhydride					398:406	maleic anhydride	391:406	maleic anhydride (MA)	391:411	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	2	48	theme	thin	355:358	arg1	nanocrystals					427:438	cellulose nanocrystals	417:438	cellulose nanocrystals (CNC)	417:444	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	2	48	theme	thin	355:358	arg1	films					374:378	thin nanocomposite films	355:378	thin nanocomposite films	355:378	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	3	49	theme	unique	630:635	arg1	morphologies					653:664	unique and more stable morphologies	630:664	unique and more stable morphologies	630:664	Alternatively, the transformation of deposited plasma films into crystalline structures introduces unique and more stable morphologies.					
36080450	3	50	theme	stable	646:651	arg1	morphologies					653:664	unique and more stable morphologies	630:664	unique and more stable morphologies	630:664	Alternatively, the transformation of deposited plasma films into crystalline structures introduces unique and more stable morphologies.					
36080450	7	51	theme	film	1432:1435	arg1	stability					1437:1445	the film stability	1428:1445	the film stability	1428:1445	Furthermore, the nucleating effect and local pinning of the crystallites to the substrate near CNC positions enhanced the film stability.					
36080450	4	52	theme	structural	686:695	arg1	rearrangements					697:710	the structural rearrangements	682:710	the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere	682:811	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	9	53	theme	films	1796:1800	arg1	stability					1759:1767	enhanced hydrolytic stability	1739:1767	enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films	1739:1833	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	6	54	theme	penetration	1248:1258	arg1	result					1232:1237	a result	1230:1237	a result of water penetration and interactions at the film/substrate interface	1230:1307	The structures particularly formed on hydrophilic substrates and were not observed before on the more hydrophobic substrates, as they exist as a result of water penetration and interactions at the film/substrate interface.					
36080450	4	55	theme	controlled	769:778	arg1	hydrolysis					780:789	controlled hydrolysis	769:789	controlled hydrolysis in a humid atmosphere	769:811	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	1	56	from	viewpoint	260:268	arg1	important					214:222	important	214:222	important	214:222	The creation of novel surface morphologies through thin-film patterning is important from a scientific and technological viewpoint in order to control specific surface properties.					
36080450	1	56	from	viewpoint	260:268	arg1	creation					143:150	The creation	139:150	The creation of novel surface morphologies through thin-film patterning	139:209	The creation of novel surface morphologies through thin-film patterning is important from a scientific and technological viewpoint in order to control specific surface properties.					
36080450	5	57	theme	progressive	967:977	arg1	growth					979:984	The progressive growth	963:984	The progressive growth of crystalline structures with fractal dendrites	963:1033	The progressive growth of crystalline structures with fractal dendrites was observed in confined thin films of 30 to 50 nm.					
36080450	3	58	theme	crystalline	596:606	arg1	structures					608:617	crystalline structures	596:617	crystalline structures	596:617	Alternatively, the transformation of deposited plasma films into crystalline structures introduces unique and more stable morphologies.					
36080450	4	59	theme	hydrolysis	941:950	arg1	stability					952:960	hydrolysis stability	941:960	hydrolysis stability	941:960	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	6	60	located	observed	1161:1168	arg1	substrates					1201:1210	the more hydrophobic substrates	1180:1210	the more hydrophobic substrates	1180:1210	The structures particularly formed on hydrophilic substrates and were not observed before on the more hydrophobic substrates, as they exist as a result of water penetration and interactions at the film/substrate interface.					
36080450	6	60	located	observed	1161:1168	arg2	structures					1091:1100	The structures	1087:1100	The structures	1087:1100	The structures particularly formed on hydrophilic substrates and were not observed before on the more hydrophobic substrates, as they exist as a result of water penetration and interactions at the film/substrate interface.					
36080450	7	61	theme	CNC	1405:1407	arg1	positions					1409:1417	CNC positions	1405:1417	CNC positions	1405:1417	Furthermore, the nucleating effect and local pinning of the crystallites to the substrate near CNC positions enhanced the film stability.					
36080450	1	62	theme	surface	161:167	arg1	morphologies					169:180	novel surface morphologies	155:180	novel surface morphologies	155:180	The creation of novel surface morphologies through thin-film patterning is important from a scientific and technological viewpoint in order to control specific surface properties.					
36080450	9	63	theme	pure	1818:1821	arg1	p					1823:1823	pure p	1818:1823	the pure p(MA) films	1814:1833	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	9	63	theme	pure	1818:1821	arg1	MA					1825:1826	MA	1825:1826	MA	1825:1826	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	8	64	theme	MA	1562:1563	arg1	groups					1581:1586	the MA carboxylic acid groups	1558:1586	the MA carboxylic acid groups	1558:1586	The chemical structures after hydrolysis were further examined through XPS, indicating esterification between the MA carboxylic acid groups and CNC surface.					
36080450	6	65	from	result	1232:1237	arg1	interface					1299:1307	the film/substrate interface	1280:1307	the film/substrate interface	1280:1307	The structures particularly formed on hydrophilic substrates and were not observed before on the more hydrophobic substrates, as they exist as a result of water penetration and interactions at the film/substrate interface.					
36080450	0	66	theme	Cellulose	98:106	arg1	Nanocrystals					108:119	Cellulose Nanocrystals	98:119	Cellulose Nanocrystals	98:119	Confined Crystallization of Thin Plasma-Polymerized Nanocomposite Films with Maleic Anhydride and Cellulose Nanocrystals under Hydrolysis.					
36080450	8	67	theme	acid	1576:1579	arg1	groups					1581:1586	the MA carboxylic acid groups	1558:1586	the MA carboxylic acid groups	1558:1586	The chemical structures after hydrolysis were further examined through XPS, indicating esterification between the MA carboxylic acid groups and CNC surface.					
36080450	4	68	theme	films	757:761	arg1	rearrangements					697:710	the structural rearrangements	682:710	the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere	682:811	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	9	69	theme	carboxylic	1690:1699	arg1	moieties					1701:1708	carboxylic moieties	1690:1708	carboxylic moieties	1690:1708	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	4	70	from	effects	837:843	arg1	stability					952:960	hydrolysis stability	941:960	hydrolysis stability	941:960	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	5	71	theme	fractal	1017:1023	arg1	dendrites					1025:1033	fractal dendrites	1017:1033	fractal dendrites	1017:1033	The progressive growth of crystalline structures with fractal dendrites was observed in confined thin films of 30 to 50 nm.					
36080450	9	72	theme	hydrolysis	1609:1618	arg1	kinetics					1620:1627	The hydrolysis kinetics	1605:1627	The hydrolysis kinetics	1605:1627	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	6	73	theme	hydrophilic	1125:1135	arg1	substrates					1137:1146	hydrophilic substrates	1125:1146	hydrophilic substrates	1125:1146	The structures particularly formed on hydrophilic substrates and were not observed before on the more hydrophobic substrates, as they exist as a result of water penetration and interactions at the film/substrate interface.					
36080450	5	74	with	growth	979:984	arg1	dendrites					1025:1033	fractal dendrites	1017:1033	fractal dendrites	1017:1033	The progressive growth of crystalline structures with fractal dendrites was observed in confined thin films of 30 to 50 nm.					
36080450	2	75	theme	metastable	471:480	arg1	morphologies					487:498	different metastable film morphologies	461:498	different metastable film morphologies that are difficult to control	461:528	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	4	76	theme	humid	796:800	arg1	atmosphere					802:811	a humid atmosphere	794:811	a humid atmosphere	794:811	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	2	77	theme	cellulose	417:425	arg1	CNC					441:443	CNC	441:443	CNC	441:443	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	2	77	theme	cellulose	417:425	arg1	nanocrystals					427:438	cellulose nanocrystals	417:438	cellulose nanocrystals (CNC)	417:444	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	0	78	theme	Thin	28:31	arg1	Films					66:70	Thin Plasma-Polymerized Nanocomposite Films	28:70	Thin Plasma-Polymerized Nanocomposite Films	28:70	Confined Crystallization of Thin Plasma-Polymerized Nanocomposite Films with Maleic Anhydride and Cellulose Nanocrystals under Hydrolysis.					
36080450	5	79	theme	structures	1001:1010	arg1	growth					979:984	The progressive growth	963:984	The progressive growth of crystalline structures with fractal dendrites	963:1033	The progressive growth of crystalline structures with fractal dendrites was observed in confined thin films of 30 to 50 nm.					
36080450	7	80	theme	local	1349:1353	arg1	pinning					1355:1361	local pinning	1349:1361	local pinning	1349:1361	Furthermore, the nucleating effect and local pinning of the crystallites to the substrate near CNC positions enhanced the film stability.					
36080450	0	81	theme	Nanocomposite	52:64	arg1	Films					66:70	Thin Plasma-Polymerized Nanocomposite Films	28:70	Thin Plasma-Polymerized Nanocomposite Films	28:70	Confined Crystallization of Thin Plasma-Polymerized Nanocomposite Films with Maleic Anhydride and Cellulose Nanocrystals under Hydrolysis.					
36080450	7	82	theme	crystallites	1370:1381	arg1	effect					1338:1343	nucleating effect	1327:1343	nucleating effect	1327:1343	Furthermore, the nucleating effect and local pinning of the crystallites to the substrate near CNC positions enhanced the film stability.					
36080450	7	82	theme	crystallites	1370:1381	arg1	pinning					1355:1361	local pinning	1349:1361	local pinning	1349:1361	Furthermore, the nucleating effect and local pinning of the crystallites to the substrate near CNC positions enhanced the film stability.					
36080450	4	83	theme	duty	871:874	arg1	cycle					876:880	low duty cycle	867:880	low duty cycle	867:880	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	4	83	theme	duty	871:874	arg1	power					892:896	variable power	883:896	variable power	883:896	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	9	84	theme	anhydride	1668:1676	arg1	groups					1678:1683	anhydride groups	1668:1683	anhydride groups	1668:1683	The hydrolysis kinetics were quantified from the conversion of anhydride groups into carboxylic moieties by FTIR analysis, indicating enhanced hydrolytic stability of p(MA+CNC) nanocomposite films relative to the pure p(MA) films.					
36080450	0	85	with	Crystallization	9:23	arg1	Anhydride					84:92	Maleic Anhydride	77:92	Maleic Anhydride	77:92	Confined Crystallization of Thin Plasma-Polymerized Nanocomposite Films with Maleic Anhydride and Cellulose Nanocrystals under Hydrolysis.					
36080450	0	85	with	Crystallization	9:23	arg1	Nanocrystals					108:119	Cellulose Nanocrystals	98:119	Cellulose Nanocrystals	98:119	Confined Crystallization of Thin Plasma-Polymerized Nanocomposite Films with Maleic Anhydride and Cellulose Nanocrystals under Hydrolysis.					
36080450	2	86	theme	maleic	391:396	arg1	MA					409:410	MA	409:410	MA	409:410	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	2	86	theme	maleic	391:396	arg1	anhydride					398:406	maleic anhydride	391:406	maleic anhydride (MA)	391:411	The pulsed-plasma polymerization of thin nanocomposite films, including maleic anhydride (MA) and cellulose nanocrystals (CNC), may result in different metastable film morphologies that are difficult to control.					
36080450	7	87	dep	effect	1338:1343	arg1	the					1323:1325	the	1323:1325	the	1323:1325	Furthermore, the nucleating effect and local pinning of the crystallites to the substrate near CNC positions enhanced the film stability.					
36080450	6	88	theme	interactions	1264:1275	arg1	result					1232:1237	a result	1230:1237	a result of water penetration and interactions at the film/substrate interface	1230:1307	The structures particularly formed on hydrophilic substrates and were not observed before on the more hydrophobic substrates, as they exist as a result of water penetration and interactions at the film/substrate interface.					
36080450	4	89	theme	plasma	848:853	arg1	conditions					855:864	plasma conditions	848:864	plasma conditions (low duty cycle, variable power)	848:897	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	4	90	from	hydrolysis	780:789	arg1	atmosphere					802:811	a humid atmosphere	794:811	a humid atmosphere	794:811	In this study, the structural rearrangements of plasma-polymerized (MA+CNC) nanocomposite films after controlled hydrolysis in a humid atmosphere were studied, including effects of plasma conditions (low duty cycle, variable power) and monomer composition (ratio MA/CNC) on hydrolysis stability.					
36080450	5	91	theme	thin	1060:1063	arg1	films					1065:1069	confined thin films	1051:1069	confined thin films of 30 to 50 nm	1051:1084	The progressive growth of crystalline structures with fractal dendrites was observed in confined thin films of 30 to 50 nm.					
36076467	0	0	theme	macroscopic	74:84	arg1	characteristics					86:100	microcosmic, mesoscopic, or macroscopic characteristics	46:100	microcosmic, mesoscopic, or macroscopic characteristics of dough	46:109	Impact of garlic oligosaccharide fractions on microcosmic, mesoscopic, or macroscopic characteristics of dough.					
36076467	6	1	theme	dough	1376:1380	arg1	matrix					1382:1387	the dough matrix	1372:1387	the dough matrix	1372:1387	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	2	2	dep	infrared	456:463	arg1	FTIR					466:469	FTIR	466:469	FTIR	466:469	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	2	3	theme	molecular	317:325	arg1	weight					327:332	molecular weight	317:332	molecular weight	317:332	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	4	4	theme	electron	1064:1071	arg1	microscopy					1073:1082	scanning electron microscopy	1055:1082	scanning electron microscopy	1055:1082	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	7	5	theme	products	1566:1573	arg1	development					1539:1549	the development	1535:1549	the development of flour-based products	1535:1573	The present study results can provide a theoretical basis for the application of garlic oligosaccharides in the development of flour-based products.					
36076467	2	6	dep	Fourier-transform	438:454	arg1	infrared					456:463	infrared	456:463	infrared	456:463	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	6	7	theme	most	1348:1351	arg1	compatibility					1353:1365	the most compatibility	1344:1365	the most compatibility	1344:1365	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	4	8	theme	confocal	1085:1092	arg1	microscopy					1109:1118	confocal laser scanning microscopy	1085:1118	confocal laser scanning microscopy	1085:1118	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	4	9	theme	thermodynamic	870:882	arg1	parameters					884:893	thermodynamic parameters	870:893	thermodynamic parameters	870:893	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	6	10	dep	HGOS	1290:1293	arg1	the					1286:1288	the	1286:1288	the	1286:1288	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	0	11	from	Impact	0:5	arg1	characteristics					86:100	microcosmic, mesoscopic, or macroscopic characteristics	46:100	microcosmic, mesoscopic, or macroscopic characteristics of dough	46:109	Impact of garlic oligosaccharide fractions on microcosmic, mesoscopic, or macroscopic characteristics of dough.					
36076467	6	12	theme	higher	1234:1239	arg1	weight					1251:1256	a slightly higher molecular weight	1223:1256	a slightly higher molecular weight (3.00 × 103 Da)	1223:1272	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	6	12	theme	higher	1234:1239	arg1	Da					1270:1271	3.00 × 103 Da	1259:1271	3.00 × 103 Da	1259:1271	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	4	13	theme	structure	953:961	arg1	terms					820:824	terms	820:824	terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure	820:961	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	4	14	theme	parameters	884:893	arg1	terms					820:824	terms	820:824	terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure	820:961	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	4	15	theme	dough	793:797	arg1	characteristics					760:774	the microcosmic, mesoscopic, and macroscopic characteristics	715:774	the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough	715:797	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	4	16	theme	differential	1005:1016	arg1	calorimetry					1027:1037	differential scanning calorimetry	1005:1037	differential scanning calorimetry	1005:1037	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	7	17	theme	flour-based	1554:1564	arg1	products					1566:1573	flour-based products	1554:1573	flour-based products	1554:1573	The present study results can provide a theoretical basis for the application of garlic oligosaccharides in the development of flour-based products.					
36076467	2	18	theme	resonance	494:502	arg1	analyses					510:517	nuclear magnetic resonance (NMR) analyses	477:517	nuclear magnetic resonance (NMR) analyses	477:517	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	7	19	theme	garlic	1508:1513	arg1	oligosaccharides					1515:1530	garlic oligosaccharides	1508:1530	garlic oligosaccharides	1508:1530	The present study results can provide a theoretical basis for the application of garlic oligosaccharides in the development of flour-based products.					
36076467	1	20	theme	purple	170:175	arg1	PGOS					185:188	PGOS	185:188	PGOS	185:188	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	1	20	theme	purple	170:175	arg1	Threeoligosaccharides					112:132	Threeoligosaccharides	112:132	Threeoligosaccharides	112:132	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	1	20	theme	purple	170:175	arg1	garlic					177:182	purple garlic	170:182	purple garlic (PGOS)	170:189	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	0	21	theme	dough	105:109	arg1	characteristics					86:100	microcosmic, mesoscopic, or macroscopic characteristics	46:100	microcosmic, mesoscopic, or macroscopic characteristics of dough	46:109	Impact of garlic oligosaccharide fractions on microcosmic, mesoscopic, or macroscopic characteristics of dough.					
36076467	7	22	theme	study	1439:1443	arg1	results					1445:1451	The present study results	1427:1451	The present study results	1427:1451	The present study results can provide a theoretical basis for the application of garlic oligosaccharides in the development of flour-based products.					
36076467	4	23	theme	physical	833:840	arg1	properties					858:867	the physical and rheological properties	829:867	properties	858:867	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	6	24	theme	dough	1412:1416	arg1	quality					1418:1424	the dough quality	1408:1424	the dough quality	1408:1424	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	4	25	theme	profile	977:983	arg1	analysis					985:992	texture profile analysis	969:992	texture profile analysis	969:992	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	6	26	theme	PGOS	1299:1302	arg1	fractions					1304:1312	PGOS fractions	1299:1312	PGOS fractions	1299:1312	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	4	27	theme	properties	858:867	arg1	terms					820:824	terms	820:824	terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure	820:961	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	4	28	theme	mesoscopic	732:741	arg1	characteristics					760:774	the microcosmic, mesoscopic, and macroscopic characteristics	715:774	the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough	715:797	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	3	29	from	proportions	606:616	arg1	oligosaccharides					576:591	garlic oligosaccharides	569:591	garlic oligosaccharides at different proportions	569:616	Moreover, fortified dough was prepared by adding garlic oligosaccharides at different proportions to evaluate the interaction of the obtained oligosaccharides with gluten proteins.					
36076467	2	30	theme	oligosaccharides	344:359	arg1	composition					301:311	chemical composition	292:311	chemical composition	292:311	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	2	30	theme	oligosaccharides	344:359	arg1	weight					327:332	molecular weight	317:332	molecular weight	317:332	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	4	31	theme	low-field	1040:1048	arg1	NMR					1050:1052	low-field NMR	1040:1052	low-field NMR	1040:1052	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	4	32	theme	rheological	846:856	arg1	properties					858:867	the physical and rheological properties	829:867	properties	858:867	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	2	33	theme	garlic	337:342	arg1	oligosaccharides					344:359	garlic oligosaccharides	337:359	garlic oligosaccharides	337:359	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	0	34	theme	oligosaccharide	17:31	arg1	fractions					33:41	garlic oligosaccharide fractions	10:41	garlic oligosaccharide fractions	10:41	Impact of garlic oligosaccharide fractions on microcosmic, mesoscopic, or macroscopic characteristics of dough.					
36076467	2	35	dep	composition	301:311	arg1	The					288:290	The	288:290	The	288:290	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	4	36	theme	FTIR	1124:1127	arg1	analyses					1129:1136	FTIR analyses	1124:1136	FTIR analyses	1124:1136	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	2	37	theme	high-performance	399:414	arg1	chromatography					423:436	gas chromatograph, high-performance liquid chromatography	380:436	chromatography	423:436	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	3	38	theme	fortified	530:538	arg1	dough					540:544	fortified dough	530:544	fortified dough	530:544	Moreover, fortified dough was prepared by adding garlic oligosaccharides at different proportions to evaluate the interaction of the obtained oligosaccharides with gluten proteins.					
36076467	4	39	theme	scanning	1055:1062	arg1	microscopy					1073:1082	scanning electron microscopy	1055:1082	scanning electron microscopy	1055:1082	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	2	40	theme	chromatograph	384:396	arg1	chromatography					423:436	gas chromatograph, high-performance liquid chromatography	380:436	chromatography	423:436	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	4	41	theme	scanning	1018:1025	arg1	calorimetry					1027:1037	differential scanning calorimetry	1005:1037	differential scanning calorimetry	1005:1037	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	1	42	theme	water	244:248	arg1	extraction					250:259	water extraction	244:259	water extraction	244:259	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	4	43	theme	secondary	943:951	arg1	structure					953:961	protein secondary structure	935:961	protein secondary structure	935:961	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	6	44	theme	SGOS	1205:1208	arg1	fraction					1210:1217	SGOS fraction	1205:1217	SGOS fraction	1205:1217	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	3	45	with	interaction	634:644	arg1	proteins					691:698	gluten proteins	684:698	gluten proteins	684:698	Moreover, fortified dough was prepared by adding garlic oligosaccharides at different proportions to evaluate the interaction of the obtained oligosaccharides with gluten proteins.					
36076467	2	46	theme	gas	380:382	arg1	chromatography					423:436	gas chromatograph, high-performance liquid chromatography	380:436	chromatography	423:436	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	0	47	theme	fractions	33:41	arg1	Impact					0:5	Impact	0:5	Impact of garlic oligosaccharide fractions on microcosmic, mesoscopic, or macroscopic characteristics of dough.	0:110	Impact of garlic oligosaccharide fractions on microcosmic, mesoscopic, or macroscopic characteristics of dough.					
36076467	6	48	contain	had	1219:1221	arg2	Da					1270:1271	3.00 × 103 Da	1259:1271	3.00 × 103 Da	1259:1271	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	6	48	contain	had	1219:1221	arg2	weight					1251:1256	a slightly higher molecular weight	1223:1256	a slightly higher molecular weight (3.00 × 103 Da)	1223:1272	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	6	48	contain	had	1219:1221	arg1	fraction					1210:1217	SGOS fraction	1205:1217	SGOS fraction	1205:1217	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	4	49	theme	protein	935:941	arg1	structure					953:961	protein secondary structure	935:961	protein secondary structure	935:961	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	4	50	theme	macroscopic	748:758	arg1	characteristics					760:774	the microcosmic, mesoscopic, and macroscopic characteristics	715:774	the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough	715:797	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	2	51	theme	liquid	416:421	arg1	chromatography					423:436	gas chromatograph, high-performance liquid chromatography	380:436	chromatography	423:436	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	2	52	theme	magnetic	485:492	arg1	resonance					494:502	nuclear magnetic resonance	477:502	nuclear magnetic resonance (NMR) analyses	477:517	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	2	52	theme	magnetic	485:492	arg1	NMR					505:507	NMR	505:507	NMR	505:507	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	4	53	theme	distribution	905:916	arg1	terms					820:824	terms	820:824	terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure	820:961	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	7	54	from	application	1493:1503	arg1	development					1539:1549	the development	1535:1549	the development of flour-based products	1535:1573	The present study results can provide a theoretical basis for the application of garlic oligosaccharides in the development of flour-based products.					
36076467	0	55	theme	microcosmic	46:56	arg1	characteristics					86:100	microcosmic, mesoscopic, or macroscopic characteristics	46:100	microcosmic, mesoscopic, or macroscopic characteristics of dough	46:109	Impact of garlic oligosaccharide fractions on microcosmic, mesoscopic, or macroscopic characteristics of dough.					
36076467	1	56	theme	ethanol	265:271	arg1	precipitation					273:285	ethanol precipitation	265:285	ethanol precipitation	265:285	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	4	57	theme	moisture	896:903	arg1	distribution					905:916	moisture distribution	896:916	moisture distribution	896:916	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	3	58	theme	obtained	653:660	arg1	oligosaccharides					662:677	the obtained oligosaccharides	649:677	the obtained oligosaccharides	649:677	Moreover, fortified dough was prepared by adding garlic oligosaccharides at different proportions to evaluate the interaction of the obtained oligosaccharides with gluten proteins.					
36076467	6	59	theme	molecular	1241:1249	arg1	weight					1251:1256	a slightly higher molecular weight	1223:1256	a slightly higher molecular weight (3.00 × 103 Da)	1223:1272	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	6	59	theme	molecular	1241:1249	arg1	Da					1270:1271	3.00 × 103 Da	1259:1271	3.00 × 103 Da	1259:1271	SGOS fraction had a slightly higher molecular weight (3.00 × 103 Da) compared to the HGOS and PGOS fractions (2.90 × 103 Da), which showed the most compatibility with the dough matrix, thereby improving the dough quality.					
36076467	2	60	theme	chemical	292:299	arg1	composition					301:311	chemical composition	292:311	chemical composition	292:311	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	4	61	theme	scanning	1100:1107	arg1	microscopy					1109:1118	confocal laser scanning microscopy	1085:1118	confocal laser scanning microscopy	1085:1118	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	4	62	theme	morphology	919:928	arg1	terms					820:824	terms	820:824	terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure	820:961	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	3	63	theme	oligosaccharides	662:677	arg1	interaction					634:644	the interaction	630:644	the interaction of the obtained oligosaccharides with gluten proteins	630:698	Moreover, fortified dough was prepared by adding garlic oligosaccharides at different proportions to evaluate the interaction of the obtained oligosaccharides with gluten proteins.					
36076467	3	64	theme	different	596:604	arg1	proportions					606:616	different proportions	596:616	different proportions	596:616	Moreover, fortified dough was prepared by adding garlic oligosaccharides at different proportions to evaluate the interaction of the obtained oligosaccharides with gluten proteins.					
36076467	3	65	theme	garlic	569:574	arg1	oligosaccharides					576:591	garlic oligosaccharides	569:591	garlic oligosaccharides at different proportions	569:616	Moreover, fortified dough was prepared by adding garlic oligosaccharides at different proportions to evaluate the interaction of the obtained oligosaccharides with gluten proteins.					
36076467	4	66	theme	microcosmic	719:729	arg1	characteristics					760:774	the microcosmic, mesoscopic, and macroscopic characteristics	715:774	the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough	715:797	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	4	67	theme	laser	1094:1098	arg1	microscopy					1109:1118	confocal laser scanning microscopy	1085:1118	confocal laser scanning microscopy	1085:1118	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	4	68	theme	fortified	783:791	arg1	dough					793:797	the fortified dough	779:797	the fortified dough	779:797	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
36076467	1	69	theme	single	142:147	arg1	garlic					155:160	single clove garlic	142:160	single clove garlic (SGOS)	142:167	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	1	69	theme	single	142:147	arg1	Threeoligosaccharides					112:132	Threeoligosaccharides	112:132	Threeoligosaccharides	112:132	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	1	69	theme	single	142:147	arg1	SGOS					163:166	SGOS	163:166	SGOS	163:166	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	1	70	theme	Cangshan	196:203	arg1	HGOS					220:223	HGOS	220:223	HGOS	220:223	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	1	70	theme	Cangshan	196:203	arg1	Threeoligosaccharides					112:132	Threeoligosaccharides	112:132	Threeoligosaccharides	112:132	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	1	70	theme	Cangshan	196:203	arg1	garlic					212:217	Cangshan hybrid garlic	196:217	Cangshan hybrid garlic (HGOS)	196:224	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	2	71	theme	nuclear	477:483	arg1	resonance					494:502	nuclear magnetic resonance	477:502	nuclear magnetic resonance (NMR) analyses	477:517	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	2	71	theme	nuclear	477:483	arg1	NMR					505:507	NMR	505:507	NMR	505:507	The chemical composition and molecular weight of garlic oligosaccharides were elucidated by gas chromatograph, high-performance liquid chromatography,Fourier-transform infrared (FTIR), and nuclear magnetic resonance (NMR) analyses.					
36076467	7	72	theme	present	1431:1437	arg1	results					1445:1451	The present study results	1427:1451	The present study results	1427:1451	The present study results can provide a theoretical basis for the application of garlic oligosaccharides in the development of flour-based products.					
36076467	3	73	theme	gluten	684:689	arg1	proteins					691:698	gluten proteins	684:698	gluten proteins	684:698	Moreover, fortified dough was prepared by adding garlic oligosaccharides at different proportions to evaluate the interaction of the obtained oligosaccharides with gluten proteins.					
36076467	1	74	theme	clove	149:153	arg1	garlic					155:160	single clove garlic	142:160	single clove garlic (SGOS)	142:167	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	1	74	theme	clove	149:153	arg1	Threeoligosaccharides					112:132	Threeoligosaccharides	112:132	Threeoligosaccharides	112:132	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	1	74	theme	clove	149:153	arg1	SGOS					163:166	SGOS	163:166	SGOS	163:166	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	7	75	theme	theoretical	1467:1477	arg1	basis					1479:1483	a theoretical basis	1465:1483	a theoretical basis for the application of garlic oligosaccharides in the development of flour-based products	1465:1573	The present study results can provide a theoretical basis for the application of garlic oligosaccharides in the development of flour-based products.					
36076467	1	76	theme	hybrid	205:210	arg1	HGOS					220:223	HGOS	220:223	HGOS	220:223	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	1	76	theme	hybrid	205:210	arg1	Threeoligosaccharides					112:132	Threeoligosaccharides	112:132	Threeoligosaccharides	112:132	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	1	76	theme	hybrid	205:210	arg1	garlic					212:217	Cangshan hybrid garlic	196:217	Cangshan hybrid garlic (HGOS)	196:224	Threeoligosaccharides, namely single clove garlic (SGOS), purple garlic (PGOS), and Cangshan hybrid garlic (HGOS), were prepared by water extraction and ethanol precipitation.					
36076467	0	77	theme	mesoscopic	59:68	arg1	characteristics					86:100	microcosmic, mesoscopic, or macroscopic characteristics	46:100	microcosmic, mesoscopic, or macroscopic characteristics of dough	46:109	Impact of garlic oligosaccharide fractions on microcosmic, mesoscopic, or macroscopic characteristics of dough.					
36076467	0	78	theme	garlic	10:15	arg1	fractions					33:41	garlic oligosaccharide fractions	10:41	garlic oligosaccharide fractions	10:41	Impact of garlic oligosaccharide fractions on microcosmic, mesoscopic, or macroscopic characteristics of dough.					
36076467	7	79	theme	oligosaccharides	1515:1530	arg1	application					1493:1503	the application	1489:1503	the application of garlic oligosaccharides in the development of flour-based products	1489:1573	The present study results can provide a theoretical basis for the application of garlic oligosaccharides in the development of flour-based products.					
36076467	4	80	theme	texture	969:975	arg1	analysis					985:992	texture profile analysis	969:992	texture profile analysis	969:992	Additionally, the microcosmic, mesoscopic, and macroscopic characteristics of the fortified dough were investigated in terms of the physical and rheological properties, thermodynamic parameters, moisture distribution, morphology, and protein secondary structure using texture profile analysis, rheology, differential scanning calorimetry, low-field NMR, scanning electron microscopy, confocal laser scanning microscopy and FTIR analyses.					
35777507	0	0	theme	Xylan/quaternized	73:89	arg1	films					125:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.					
35777507	1	1	theme	film-forming	326:337	arg1	property					339:346	poor film-forming property	321:346	poor film-forming property	321:346	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	5	2	theme	films	1121:1125	arg1	rate					1109:1112	The bacteriostasis rate	1090:1112	The bacteriostasis rate of the films against E. coli and S. aureus	1090:1155	The bacteriostasis rate of the films against E. coli and S. aureus reached up to 99 %.					
35777507	7	3	theme	fabricated	1348:1357	arg1	films					1369:1373	the fabricated composite films	1344:1373	the fabricated composite films	1344:1373	In conclusion, all the results ensure that the fabricated composite films have considerable promising application in the food packaging industry.					
35777507	4	4	theme	antibacterial	1051:1063	arg1	properties					1065:1074	the antibacterial properties	1047:1074	the ultraviolet shielding performance as well as the antibacterial properties of the films	998:1087	The addition of nano ZnO@Xylan decreased water vapor permeability and solubility, meanwhile obviously increased the ultraviolet shielding performance as well as the antibacterial properties of the films.					
35777507	0	5	theme	@	72:72	arg1	films					125:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.					
35777507	1	6	theme	property	339:346	arg1	drawbacks					308:316	the drawbacks	304:316	the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property	304:426	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	1	7	theme	good	163:166	arg1	candidate					178:186	a good potential candidate	161:186	a good potential candidate for food packaging film	161:210	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	1	7	theme	good	163:166	arg1	Xylan					132:136	Xylan	132:136	Xylan	132:136	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	3	8	theme	crystallography	827:841	arg1	structure					843:851	crystallography structure	827:851	crystallography structure	827:851	The surface morphology, molecular structure and crystallography structure of the films were characterized.					
35777507	0	9	theme	alcohol	107:113	arg1	films					125:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.					
35777507	5	10	dep	99 	1171:1173	arg1	to					1168:1169	to	1168:1169	to	1168:1169	The bacteriostasis rate of the films against E. coli and S. aureus reached up to 99 %.					
35777507	5	11	theme	bacteriostasis	1094:1107	arg1	rate					1109:1112	The bacteriostasis rate	1090:1112	The bacteriostasis rate of the films against E. coli and S. aureus	1090:1155	The bacteriostasis rate of the films against E. coli and S. aureus reached up to 99 %.					
35777507	3	12	theme	molecular	803:811	arg1	structure					813:821	molecular structure	803:821	molecular structure	803:821	The surface morphology, molecular structure and crystallography structure of the films were characterized.					
35777507	0	13	theme	xylan/polyvinyl	91:105	arg1	films					125:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.					
35777507	1	14	theme	humidity	349:356	arg1	sensitivity					358:368	humidity sensitivity	349:368	humidity sensitivity	349:368	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	2	15	theme	@	581:581	arg1	Xylan					582:586	nano ZnO@Xylan	573:586	nano ZnO@Xylan	573:586	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	2	15	theme	@	581:581	arg1	xylan					566:570	xylan	566:570	xylan (nano ZnO@Xylan)	566:587	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	6	16	theme	Xylan/QX/PVA	1248:1259	arg1	films					1261:1265	ZnO@Xylan/QX/PVA films	1244:1265	ZnO@Xylan/QX/PVA films	1244:1265	Furthermore, the preservation time of cherry tomatoes covered with ZnO@Xylan/QX/PVA films was extended to at least 21 days.					
35777507	7	17	theme	food	1422:1425	arg1	industry					1437:1444	the food packaging industry	1418:1444	the food packaging industry	1418:1444	In conclusion, all the results ensure that the fabricated composite films have considerable promising application in the food packaging industry.					
35777507	2	18	theme	nano	763:766	arg1	Xylan					772:776	nano ZnO@Xylan	763:776	nano ZnO@Xylan	763:776	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	0	19	theme	films	125:129	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.					
35777507	0	19	theme	films	125:129	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.					
35777507	0	19	theme	films	125:129	arg1	application					49:59	food packaging application	34:59	food packaging application	34:59	Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.					
35777507	1	20	theme	sensitivity	358:368	arg1	drawbacks					308:316	the drawbacks	304:316	the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property	304:426	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	6	21	theme	@	1247:1247	arg1	films					1261:1265	ZnO@Xylan/QX/PVA films	1244:1265	ZnO@Xylan/QX/PVA films	1244:1265	Furthermore, the preservation time of cherry tomatoes covered with ZnO@Xylan/QX/PVA films was extended to at least 21 days.					
35777507	1	22	theme	potential	168:176	arg1	candidate					178:186	a good potential candidate	161:186	a good potential candidate for food packaging film	161:210	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	1	22	theme	potential	168:176	arg1	Xylan					132:136	Xylan	132:136	Xylan	132:136	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	0	23	theme	composite	115:123	arg1	films					125:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.					
35777507	7	24	theme	packaging	1427:1435	arg1	industry					1437:1444	the food packaging industry	1418:1444	the food packaging industry	1418:1444	In conclusion, all the results ensure that the fabricated composite films have considerable promising application in the food packaging industry.					
35777507	2	25	theme	nanoprecipitation	605:621	arg1	method					623:628	nanoprecipitation method	605:628	nanoprecipitation method	605:628	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	2	26	theme	films	675:679	arg1	series					641:646	a series	639:646	a series of biodegradable composite films	639:679	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	1	27	theme	weak	371:374	arg1	strength					387:394	weak mechanical strength	371:394	weak mechanical strength	371:394	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	2	28	theme	composite	665:673	arg1	films					675:679	biodegradable composite films	651:679	biodegradable composite films	651:679	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	7	29	theme	considerable	1380:1391	arg1	application					1403:1413	considerable promising application	1380:1413	considerable promising application	1380:1413	In conclusion, all the results ensure that the fabricated composite films have considerable promising application in the food packaging industry.					
35777507	7	30	contain	have	1375:1378	arg1	films					1369:1373	the fabricated composite films	1344:1373	the fabricated composite films	1344:1373	In conclusion, all the results ensure that the fabricated composite films have considerable promising application in the food packaging industry.					
35777507	7	30	contain	have	1375:1378	arg2	application					1403:1413	considerable promising application	1380:1413	considerable promising application	1380:1413	In conclusion, all the results ensure that the fabricated composite films have considerable promising application in the food packaging industry.					
35777507	2	31	theme	biodegradable	651:663	arg1	films					675:679	biodegradable composite films	651:679	biodegradable composite films	651:679	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	7	32	theme	promising	1393:1401	arg1	application					1403:1413	considerable promising application	1380:1413	considerable promising application	1380:1413	In conclusion, all the results ensure that the fabricated composite films have considerable promising application in the food packaging industry.					
35777507	1	33	theme	food	192:195	arg1	packaging					197:205	food packaging film	192:210	food packaging film	192:210	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	4	34	theme	Xylan	911:915	arg1	addition					890:897	The addition	886:897	The addition of nano ZnO@Xylan	886:915	The addition of nano ZnO@Xylan decreased water vapor permeability and solubility, meanwhile obviously increased the ultraviolet shielding performance as well as the antibacterial properties of the films.					
35777507	4	35	theme	ultraviolet	1002:1012	arg1	performance					1024:1034	the ultraviolet shielding performance	998:1034	the ultraviolet shielding performance as well as the antibacterial properties of the films	998:1087	The addition of nano ZnO@Xylan decreased water vapor permeability and solubility, meanwhile obviously increased the ultraviolet shielding performance as well as the antibacterial properties of the films.					
35777507	2	36	theme	Xylan	772:776	arg1	incorporation					746:758	incorporation	746:758	incorporation of nano ZnO@Xylan	746:776	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	4	37	theme	@	910:910	arg1	Xylan					911:915	nano ZnO@Xylan	902:915	nano ZnO@Xylan	902:915	The addition of nano ZnO@Xylan decreased water vapor permeability and solubility, meanwhile obviously increased the ultraviolet shielding performance as well as the antibacterial properties of the films.					
35777507	1	38	theme	mechanical	376:385	arg1	strength					387:394	weak mechanical strength	371:394	weak mechanical strength	371:394	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	2	39	theme	@	771:771	arg1	Xylan					772:776	nano ZnO@Xylan	763:776	nano ZnO@Xylan	763:776	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	0	40	theme	packaging	39:47	arg1	application					49:59	food packaging application	34:59	food packaging application	34:59	Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.					
35777507	4	41	theme	water	927:931	arg1	permeability					939:950	water vapor permeability	927:950	water vapor permeability	927:950	The addition of nano ZnO@Xylan decreased water vapor permeability and solubility, meanwhile obviously increased the ultraviolet shielding performance as well as the antibacterial properties of the films.					
35777507	1	42	theme	strength	387:394	arg1	drawbacks					308:316	the drawbacks	304:316	the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property	304:426	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	6	43	theme	tomatoes	1222:1229	arg1	time					1207:1210	the preservation time	1190:1210	the preservation time of cherry tomatoes covered with ZnO@Xylan/QX/PVA films	1190:1265	Furthermore, the preservation time of cherry tomatoes covered with ZnO@Xylan/QX/PVA films was extended to at least 21 days.					
35777507	2	44	theme	ZnO	768:770	arg1	Xylan					772:776	nano ZnO@Xylan	763:776	nano ZnO@Xylan	763:776	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	0	45	theme	food	34:37	arg1	application					49:59	food packaging application	34:59	food packaging application	34:59	Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.					
35777507	2	46	theme	ZnO	532:534	arg1	nanoparticles					536:548	then ZnO nanoparticles	527:548	then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan)	527:587	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	3	47	dep	morphology	791:800	arg1	The					779:781	The	779:781	The	779:781	The surface morphology, molecular structure and crystallography structure of the films were characterized.					
35777507	6	48	theme	cherry	1215:1220	arg1	tomatoes					1222:1229	cherry tomatoes	1215:1229	cherry tomatoes covered with ZnO@Xylan/QX/PVA films	1215:1265	Furthermore, the preservation time of cherry tomatoes covered with ZnO@Xylan/QX/PVA films was extended to at least 21 days.					
35777507	2	49	theme	quaternized	701:711	arg1	xylan					713:717	quaternized xylan	701:717	quaternized xylan	701:717	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	2	50	theme	film-forming	504:515	arg1	property					517:524	the film-forming property	500:524	the film-forming property	500:524	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	2	51	theme	ZnO	578:580	arg1	Xylan					582:586	nano ZnO@Xylan	573:586	nano ZnO@Xylan	573:586	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	2	51	theme	ZnO	578:580	arg1	xylan					566:570	xylan	566:570	xylan (nano ZnO@Xylan)	566:587	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	1	52	theme	poor	400:403	arg1	property					419:426	poor antibacterial property	400:426	poor antibacterial property	400:426	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	4	53	theme	shielding	1014:1022	arg1	performance					1024:1034	the ultraviolet shielding performance	998:1034	the ultraviolet shielding performance as well as the antibacterial properties of the films	998:1087	The addition of nano ZnO@Xylan decreased water vapor permeability and solubility, meanwhile obviously increased the ultraviolet shielding performance as well as the antibacterial properties of the films.					
35777507	2	54	mod	modified	462:469	arg3	quaternization					474:487	quaternization	474:487	quaternization	474:487	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	2	54	mod	modified	462:469	arg1	xylan					444:448	xylan	444:448	xylan	444:448	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	2	55	theme	nano	573:576	arg1	Xylan					582:586	nano ZnO@Xylan	573:586	nano ZnO@Xylan	573:586	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	2	55	theme	nano	573:576	arg1	xylan					566:570	xylan	566:570	xylan (nano ZnO@Xylan)	566:587	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	1	56	theme	antibacterial	405:417	arg1	property					419:426	poor antibacterial property	400:426	poor antibacterial property	400:426	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	6	57	theme	ZnO	1244:1246	arg1	films					1261:1265	ZnO@Xylan/QX/PVA films	1244:1265	ZnO@Xylan/QX/PVA films	1244:1265	Furthermore, the preservation time of cherry tomatoes covered with ZnO@Xylan/QX/PVA films was extended to at least 21 days.					
35777507	4	58	theme	ZnO	907:909	arg1	Xylan					911:915	nano ZnO@Xylan	902:915	nano ZnO@Xylan	902:915	The addition of nano ZnO@Xylan decreased water vapor permeability and solubility, meanwhile obviously increased the ultraviolet shielding performance as well as the antibacterial properties of the films.					
35777507	1	59	theme	property	419:426	arg1	drawbacks					308:316	the drawbacks	304:316	the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property	304:426	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	6	60	theme	preservation	1194:1205	arg1	time					1207:1210	the preservation time	1190:1210	the preservation time of cherry tomatoes covered with ZnO@Xylan/QX/PVA films	1190:1265	Furthermore, the preservation time of cherry tomatoes covered with ZnO@Xylan/QX/PVA films was extended to at least 21 days.					
35777507	7	61	theme	composite	1359:1367	arg1	films					1369:1373	the fabricated composite films	1344:1373	the fabricated composite films	1344:1373	In conclusion, all the results ensure that the fabricated composite films have considerable promising application in the food packaging industry.					
35777507	3	62	theme	films	860:864	arg1	morphology					791:800	surface morphology	783:800	surface morphology	783:800	The surface morphology, molecular structure and crystallography structure of the films were characterized.					
35777507	3	62	theme	films	860:864	arg1	structure					813:821	molecular structure	803:821	molecular structure	803:821	The surface morphology, molecular structure and crystallography structure of the films were characterized.					
35777507	3	62	theme	films	860:864	arg1	structure					843:851	crystallography structure	827:851	crystallography structure	827:851	The surface morphology, molecular structure and crystallography structure of the films were characterized.					
35777507	4	63	theme	nano	902:905	arg1	Xylan					911:915	nano ZnO@Xylan	902:915	nano ZnO@Xylan	902:915	The addition of nano ZnO@Xylan decreased water vapor permeability and solubility, meanwhile obviously increased the ultraviolet shielding performance as well as the antibacterial properties of the films.					
35777507	4	64	theme	vapor	933:937	arg1	permeability					939:950	water vapor permeability	927:950	water vapor permeability	927:950	The addition of nano ZnO@Xylan decreased water vapor permeability and solubility, meanwhile obviously increased the ultraviolet shielding performance as well as the antibacterial properties of the films.					
35777507	1	65	theme	vast	227:230	arg1	source					232:237	the vast source	223:237	the vast source	223:237	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	0	66	theme	ZnO	69:71	arg1	films					125:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.					
35777507	2	67	theme	polyvinyl	723:731	arg1	alcohol					733:739	polyvinyl alcohol	723:739	polyvinyl alcohol	723:739	In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan.					
35777507	4	68	theme	films	1083:1087	arg1	performance					1024:1034	the ultraviolet shielding performance	998:1034	the ultraviolet shielding performance as well as the antibacterial properties of the films	998:1087	The addition of nano ZnO@Xylan decreased water vapor permeability and solubility, meanwhile obviously increased the ultraviolet shielding performance as well as the antibacterial properties of the films.					
35777507	4	68	theme	films	1083:1087	arg1	properties					1065:1074	the antibacterial properties	1047:1074	the ultraviolet shielding performance as well as the antibacterial properties of the films	998:1087	The addition of nano ZnO@Xylan decreased water vapor permeability and solubility, meanwhile obviously increased the ultraviolet shielding performance as well as the antibacterial properties of the films.					
35777507	0	69	theme	nano	64:67	arg1	films					125:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films	64:129	Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.					
35777507	1	70	theme	poor	321:324	arg1	property					339:346	poor film-forming property	321:346	poor film-forming property	321:346	Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property.					
35777507	3	71	theme	surface	783:789	arg1	morphology					791:800	surface morphology	783:800	surface morphology	783:800	The surface morphology, molecular structure and crystallography structure of the films were characterized.					
36100751	11	0	dep	acids	1431:1435	arg1	EC:1.3.1.44					1438:1448	EC:1.3.1.44	1438:1448	EC:1.3.1.44	1438:1448	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	11	1	theme	higher	1498:1503	arg1	abundance					1514:1522	higher relative abundance	1498:1522	higher relative abundance in winter-a mechanism of natural selection and environmental adaptation	1498:1594	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	2	2	theme	gut	292:294	arg1	microbiota					296:305	the gut microbiota	288:305	the gut microbiota	288:305	Considering the lack of a comprehensive understanding of the gut microbiota, there is a need to study the gut microbiota of Siberian musk deer (SMD).					
36100751	3	3	theme	high-throughput	411:425	arg1	sequencing					427:436	high-throughput sequencing	411:436	high-throughput sequencing	411:436	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	1	4	theme	deer	214:217	arg1	breeding					197:204	artificial breeding	186:204	artificial breeding of musk deer	186:217	Musk deer are famous for their secretion of musk; however, the scale of artificial breeding of musk deer is limited.					
36100751	4	5	theme	relative	559:566	arg1	higher					610:615	higher	610:615	higher	610:615	The relative abundance of Firmicutes was significantly higher in SMD than in FMD, with a corresponding decrease in Bacteroidetes, and showed significant seasonal variation.					
36100751	4	5	theme	relative	559:566	arg1	abundance					568:576	The relative abundance	555:576	The relative abundance of Firmicutes	555:590	The relative abundance of Firmicutes was significantly higher in SMD than in FMD, with a corresponding decrease in Bacteroidetes, and showed significant seasonal variation.					
36100751	11	6	theme	winter-a	1527:1534	arg1	mechanism					1536:1544	winter-a mechanism	1527:1544	winter-a mechanism of natural selection and environmental adaptation	1527:1594	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	9	7	theme	gut	1290:1292	arg1	microbes					1294:1301	gut microbes	1290:1301	gut microbes of SMD and FMD	1290:1316	Furthermore, antibiotic resistances were identified and significantly different in gut microbes of SMD and FMD.					
36100751	5	8	theme	enriched	758:765	arg1	activity					767:774	enriched activity	758:774	enriched activity for carbohydrate metabolism	758:802	The gut microbiome of FMD has enriched activity for carbohydrate metabolism, while in SMD, amino acids and energy metabolism was higher.					
36100751	11	9	theme	fatty	1425:1429	arg1	acids					1431:1435	the short-chain fatty acids	1409:1435	the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2)	1409:1461	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	0	10	theme	Forest	96:101	arg1	deer					108:111	Forest musk deer	96:111	Forest musk deer	96:111	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	3	11	theme	gut	475:477	arg1	communities					489:499	gut microbial communities	475:499	gut microbial communities	475:499	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	15	12	theme	Seasonal	2002:2009	arg1	variations					2011:2020	Seasonal variations	2002:2020	Seasonal variations	2002:2020	• Seasonal variations alter microbial function in SMD, carbohydrate metabolism was higher in summer.					
36100751	5	13	theme	FMD	750:752	arg1	microbiome					736:745	The gut microbiome	728:745	The gut microbiome of FMD	728:752	The gut microbiome of FMD has enriched activity for carbohydrate metabolism, while in SMD, amino acids and energy metabolism was higher.					
36100751	8	14	theme	significant	1137:1147	arg1	differences					1149:1159	significant differences	1137:1159	significant differences in their microbial communities and functions	1137:1204	This indicates that FMD and SMD have significant differences in their microbial communities and functions.					
36100751	8	15	contain	have	1132:1135	arg1	FMD					1120:1122	FMD	1120:1122	FMD	1120:1122	This indicates that FMD and SMD have significant differences in their microbial communities and functions.					
36100751	8	15	contain	have	1132:1135	arg1	SMD					1128:1130	SMD	1128:1130	SMD	1128:1130	This indicates that FMD and SMD have significant differences in their microbial communities and functions.					
36100751	8	15	contain	have	1132:1135	arg2	differences					1149:1159	significant differences	1137:1159	significant differences in their microbial communities and functions	1137:1204	This indicates that FMD and SMD have significant differences in their microbial communities and functions.					
36100751	0	16	from	deer	87:90	arg1	study					12:16	Comparative study	0:16	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.	0:112	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	13	17	theme	Significant	1812:1822	arg1	differences					1824:1834	Significant differences	1812:1834	Significant differences in microbial communities	1812:1859	KEY POINTS: • Significant differences in microbial communities and their function between FMD and SMD.					
36100751	5	18	theme	amino	819:823	arg1	acids					825:829	amino acids	819:829	amino acids	819:829	The gut microbiome of FMD has enriched activity for carbohydrate metabolism, while in SMD, amino acids and energy metabolism was higher.					
36100751	6	19	theme	pyruvate	909:916	arg1	metabolism					918:927	pyruvate metabolism	909:927	pyruvate metabolism in SMD	909:934	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	8	20	theme	microbial	1170:1178	arg1	communities					1180:1190	their microbial communities	1164:1190	their microbial communities	1164:1190	This indicates that FMD and SMD have significant differences in their microbial communities and functions.					
36100751	7	21	theme	feeding	1084:1090	arg1	habits					1092:1097	feeding habits	1084:1097	feeding habits	1084:1097	were more abundant, and these were related to the living environment and feeding habits.					
36100751	3	22	theme	forest	531:536	arg1	FMD					549:551	FMD	549:551	FMD	549:551	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	3	22	theme	forest	531:536	arg1	deer					543:546	forest musk deer	531:546	forest musk deer (FMD)	531:552	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	15	23	theme	carbohydrate	2055:2066	arg1	metabolism					2068:2077	carbohydrate metabolism	2055:2077	carbohydrate metabolism	2055:2077	• Seasonal variations alter microbial function in SMD, carbohydrate metabolism was higher in summer.					
36100751	13	24	dep	POINTS	1802:1807	arg1	•					1810:1810	•	1810:1810	• Significant differences in microbial communities and their function between FMD and SMD	1810:1898	KEY POINTS: • Significant differences in microbial communities and their function between FMD and SMD.					
36100751	11	25	theme	key	1394:1396	arg1	different					1482:1490	different	1482:1490	different	1482:1490	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	11	25	theme	key	1394:1396	arg1	enzymes					1398:1404	The key enzymes	1390:1404	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2)	1390:1461	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	0	26	theme	microbiota	59:68	arg1	structure					38:46	structure	38:46	structure	38:46	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	0	26	theme	microbiota	59:68	arg1	function					25:32	function	25:32	function	25:32	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	6	27	from	metabolism	918:927	arg1	SMD					932:934	SMD	932:934	SMD	932:934	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	3	28	from	differences	460:470	arg1	functions					505:513	functions	505:513	functions	505:513	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	3	28	from	differences	460:470	arg1	communities					489:499	gut microbial communities	475:499	gut microbial communities	475:499	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	0	29	theme	Siberian	73:80	arg1	deer					87:90	Siberian musk deer	73:90	Siberian musk deer	73:90	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	4	30	from	decrease	658:665	arg1	Bacteroidetes					670:682	Bacteroidetes	670:682	Bacteroidetes	670:682	The relative abundance of Firmicutes was significantly higher in SMD than in FMD, with a corresponding decrease in Bacteroidetes, and showed significant seasonal variation.					
36100751	6	31	theme	key	869:871	arg1	reactions					883:891	The key enzymatic reactions	865:891	The key enzymatic reactions	865:891	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	6	31	theme	key	869:871	arg1	enzymes					954:960	enzymes	954:960	enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4	954:1007	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	6	31	theme	key	869:871	arg1	related					898:904	related	898:904	related	898:904	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	0	32	from	structure	38:46	arg1	deer					108:111	Forest musk deer	96:111	Forest musk deer	96:111	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	0	32	from	structure	38:46	arg1	deer					87:90	Siberian musk deer	73:90	Siberian musk deer	73:90	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	14	33	theme	Firmicutes	1955:1964	arg1	metabolism					1914:1923	The energy metabolism	1903:1923	The energy metabolism	1903:1923	• The energy metabolism and the relative abundance of Firmicutes were significantly higher in SMD.					
36100751	14	33	theme	Firmicutes	1955:1964	arg1	higher					1985:1990	higher	1985:1990	higher	1985:1990	• The energy metabolism and the relative abundance of Firmicutes were significantly higher in SMD.					
36100751	14	33	theme	Firmicutes	1955:1964	arg1	abundance					1942:1950	the relative abundance	1929:1950	the relative abundance of Firmicutes	1929:1964	• The energy metabolism and the relative abundance of Firmicutes were significantly higher in SMD.					
36100751	5	34	contain	has	754:756	arg2	activity					767:774	enriched activity	758:774	enriched activity for carbohydrate metabolism	758:802	The gut microbiome of FMD has enriched activity for carbohydrate metabolism, while in SMD, amino acids and energy metabolism was higher.					
36100751	5	34	contain	has	754:756	arg1	microbiome					736:745	The gut microbiome	728:745	The gut microbiome of FMD	728:752	The gut microbiome of FMD has enriched activity for carbohydrate metabolism, while in SMD, amino acids and energy metabolism was higher.					
36100751	9	35	theme	SMD	1306:1308	arg1	microbes					1294:1301	gut microbes	1290:1301	gut microbes of SMD and FMD	1290:1316	Furthermore, antibiotic resistances were identified and significantly different in gut microbes of SMD and FMD.					
36100751	2	36	theme	deer	369:372	arg1	microbiota					341:350	the gut microbiota	333:350	the gut microbiota of Siberian musk deer (SMD)	333:378	Considering the lack of a comprehensive understanding of the gut microbiota, there is a need to study the gut microbiota of Siberian musk deer (SMD).					
36100751	0	37	from	function	25:32	arg1	deer					108:111	Forest musk deer	96:111	Forest musk deer	96:111	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	0	37	from	function	25:32	arg1	deer					87:90	Siberian musk deer	73:90	Siberian musk deer	73:90	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	0	38	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.	0:112	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	2	39	theme	Siberian	355:362	arg1	SMD					375:377	SMD	375:377	SMD	375:377	Considering the lack of a comprehensive understanding of the gut microbiota, there is a need to study the gut microbiota of Siberian musk deer (SMD).					
36100751	2	39	theme	Siberian	355:362	arg1	deer					369:372	Siberian musk deer	355:372	Siberian musk deer (SMD)	355:378	Considering the lack of a comprehensive understanding of the gut microbiota, there is a need to study the gut microbiota of Siberian musk deer (SMD).					
36100751	12	40	theme	husbandry	1731:1739	arg1	strategies					1741:1750	conservation and husbandry strategies	1714:1750	conservation and husbandry strategies for musk deer	1714:1764	This study is the first to analyze the composition of the gut microbiota of SMD and can be used to develop or modify conservation and husbandry strategies for musk deer, to improve their productivity.					
36100751	12	41	theme	conservation	1714:1725	arg1	strategies					1741:1750	conservation and husbandry strategies	1714:1750	conservation and husbandry strategies for musk deer	1714:1764	This study is the first to analyze the composition of the gut microbiota of SMD and can be used to develop or modify conservation and husbandry strategies for musk deer, to improve their productivity.					
36100751	1	42	theme	Musk	114:117	arg1	deer					119:122	Musk deer	114:122	Musk deer	114:122	Musk deer are famous for their secretion of musk; however, the scale of artificial breeding of musk deer is limited.					
36100751	10	43	theme	microbial	1354:1362	arg1	communities					1364:1374	microbial communities	1354:1374	microbial communities	1354:1374	For SMD, seasonal variations alter microbial communities and function.					
36100751	11	44	theme	adaptation	1585:1594	arg1	mechanism					1536:1544	winter-a mechanism	1527:1544	winter-a mechanism of natural selection and environmental adaptation	1527:1594	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	0	45	theme	function	25:32	arg1	study					12:16	Comparative study	0:16	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.	0:112	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	9	46	theme	antibiotic	1220:1229	arg1	resistances					1231:1241	antibiotic resistances	1220:1241	antibiotic resistances	1220:1241	Furthermore, antibiotic resistances were identified and significantly different in gut microbes of SMD and FMD.					
36100751	9	47	from	microbes	1294:1301	arg1	different					1277:1285	different	1277:1285	different	1277:1285	Furthermore, antibiotic resistances were identified and significantly different in gut microbes of SMD and FMD.					
36100751	11	48	from	abundance	1514:1522	arg1	mechanism					1536:1544	winter-a mechanism	1527:1544	winter-a mechanism of natural selection and environmental adaptation	1527:1594	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	0	49	theme	structure	38:46	arg1	study					12:16	Comparative study	0:16	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.	0:112	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	0	50	dep	function	25:32	arg1	the					21:23	the	21:23	the	21:23	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	3	51	theme	Quantitative	381:392	arg1	analysis					398:405	Quantitative PCR analysis	381:405	Quantitative PCR analysis	381:405	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	12	52	used	used	1688:1691	arg2	study					1602:1606	This study	1597:1606	This study	1597:1606	This study is the first to analyze the composition of the gut microbiota of SMD and can be used to develop or modify conservation and husbandry strategies for musk deer, to improve their productivity.					
36100751	12	52	used	used	1688:1691	arg2	first					1615:1619	first	1615:1619	first	1615:1619	This study is the first to analyze the composition of the gut microbiota of SMD and can be used to develop or modify conservation and husbandry strategies for musk deer, to improve their productivity.					
36100751	12	53	theme	microbiota	1659:1668	arg1	composition					1636:1646	the composition	1632:1646	the composition of the gut microbiota of SMD	1632:1675	This study is the first to analyze the composition of the gut microbiota of SMD and can be used to develop or modify conservation and husbandry strategies for musk deer, to improve their productivity.					
36100751	11	54	with	different	1482:1490	arg1	abundance					1514:1522	higher relative abundance	1498:1522	higher relative abundance in winter-a mechanism of natural selection and environmental adaptation	1498:1594	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	4	55	theme	significant	696:706	arg1	variation					717:725	significant seasonal variation	696:725	significant seasonal variation	696:725	The relative abundance of Firmicutes was significantly higher in SMD than in FMD, with a corresponding decrease in Bacteroidetes, and showed significant seasonal variation.					
36100751	9	56	theme	FMD	1314:1316	arg1	microbes					1294:1301	gut microbes	1290:1301	gut microbes of SMD and FMD	1290:1316	Furthermore, antibiotic resistances were identified and significantly different in gut microbes of SMD and FMD.					
36100751	12	57	theme	SMD	1673:1675	arg1	microbiota					1659:1668	the gut microbiota	1651:1668	the gut microbiota of SMD	1651:1675	This study is the first to analyze the composition of the gut microbiota of SMD and can be used to develop or modify conservation and husbandry strategies for musk deer, to improve their productivity.					
36100751	2	58	theme	microbiota	296:305	arg1	understanding					271:283	a comprehensive understanding	255:283	a comprehensive understanding of the gut microbiota	255:305	Considering the lack of a comprehensive understanding of the gut microbiota, there is a need to study the gut microbiota of Siberian musk deer (SMD).					
36100751	1	59	theme	breeding	197:204	arg1	scale					177:181	the scale	173:181	the scale of artificial breeding of musk deer	173:217	Musk deer are famous for their secretion of musk; however, the scale of artificial breeding of musk deer is limited.					
36100751	11	60	theme	relative	1505:1512	arg1	abundance					1514:1522	higher relative abundance	1498:1522	higher relative abundance in winter-a mechanism of natural selection and environmental adaptation	1498:1594	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	11	61	theme	short-chain	1413:1423	arg1	acids					1431:1435	the short-chain fatty acids	1409:1435	the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2)	1409:1461	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	1	62	theme	musk	209:212	arg1	deer					214:217	musk deer	209:217	musk deer	209:217	Musk deer are famous for their secretion of musk; however, the scale of artificial breeding of musk deer is limited.					
36100751	11	63	theme	natural	1549:1555	arg1	selection					1557:1565	natural selection	1549:1565	natural selection	1549:1565	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	6	64	from	FMD	949:951	arg1	reactions					883:891	The key enzymatic reactions	865:891	The key enzymatic reactions	865:891	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	6	64	from	FMD	949:951	arg1	enzymes					954:960	enzymes	954:960	enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4	954:1007	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	6	64	from	FMD	949:951	arg1	related					898:904	related	898:904	related	898:904	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	15	65	theme	microbial	2028:2036	arg1	function					2038:2045	microbial function	2028:2045	microbial function	2028:2045	• Seasonal variations alter microbial function in SMD, carbohydrate metabolism was higher in summer.					
36100751	13	66	theme	KEY	1798:1800	arg1	POINTS					1802:1807	KEY POINTS	1798:1807	KEY POINTS: • Significant differences in microbial communities and their function between FMD and SMD.	1798:1899	KEY POINTS: • Significant differences in microbial communities and their function between FMD and SMD.					
36100751	3	67	theme	microbial	479:487	arg1	communities					489:499	gut microbial communities	475:499	gut microbial communities	475:499	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	0	68	theme	musk	103:106	arg1	deer					108:111	Forest musk deer	96:111	Forest musk deer	96:111	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	11	69	theme	acids	1431:1435	arg1	different					1482:1490	different	1482:1490	different	1482:1490	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	11	69	theme	acids	1431:1435	arg1	enzymes					1398:1404	The key enzymes	1390:1404	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2)	1390:1461	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	1	70	theme	musk	158:161	arg1	secretion					145:153	their secretion	139:153	their secretion of musk	139:161	Musk deer are famous for their secretion of musk; however, the scale of artificial breeding of musk deer is limited.					
36100751	13	71	from	differences	1824:1834	arg1	communities					1849:1859	microbial communities	1839:1859	microbial communities	1839:1859	KEY POINTS: • Significant differences in microbial communities and their function between FMD and SMD.					
36100751	13	72	from	function	1871:1878	arg1	communities					1849:1859	microbial communities	1839:1859	microbial communities	1839:1859	KEY POINTS: • Significant differences in microbial communities and their function between FMD and SMD.					
36100751	3	73	theme	musk	538:541	arg1	FMD					549:551	FMD	549:551	FMD	549:551	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	3	73	theme	musk	538:541	arg1	deer					543:546	forest musk deer	531:546	forest musk deer (FMD)	531:552	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	12	74	dep	first	1615:1619	arg1	analyze					1624:1630	analyze	1624:1630	to analyze the composition of the gut microbiota of SMD	1621:1675	This study is the first to analyze the composition of the gut microbiota of SMD and can be used to develop or modify conservation and husbandry strategies for musk deer, to improve their productivity.					
36100751	12	74	dep	first	1615:1619	arg1	used					1688:1691	used	1688:1691	can be used to develop or modify conservation and husbandry strategies for musk deer, to improve their productivity	1681:1795	This study is the first to analyze the composition of the gut microbiota of SMD and can be used to develop or modify conservation and husbandry strategies for musk deer, to improve their productivity.					
36100751	2	75	theme	understanding	271:283	arg1	lack					247:250	the lack	243:250	the lack of a comprehensive understanding of the gut microbiota	243:305	Considering the lack of a comprehensive understanding of the gut microbiota, there is a need to study the gut microbiota of Siberian musk deer (SMD).					
36100751	0	76	theme	gut	55:57	arg1	microbiota					59:68	the gut microbiota	51:68	the gut microbiota	51:68	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	5	77	theme	gut	732:734	arg1	microbiome					736:745	The gut microbiome	728:745	The gut microbiome of FMD	728:752	The gut microbiome of FMD has enriched activity for carbohydrate metabolism, while in SMD, amino acids and energy metabolism was higher.					
36100751	5	78	theme	carbohydrate	780:791	arg1	metabolism					793:802	carbohydrate metabolism	780:802	carbohydrate metabolism	780:802	The gut microbiome of FMD has enriched activity for carbohydrate metabolism, while in SMD, amino acids and energy metabolism was higher.					
36100751	4	79	theme	Firmicutes	581:590	arg1	higher					610:615	higher	610:615	higher	610:615	The relative abundance of Firmicutes was significantly higher in SMD than in FMD, with a corresponding decrease in Bacteroidetes, and showed significant seasonal variation.					
36100751	4	79	theme	Firmicutes	581:590	arg1	abundance					568:576	The relative abundance	555:576	The relative abundance of Firmicutes	555:590	The relative abundance of Firmicutes was significantly higher in SMD than in FMD, with a corresponding decrease in Bacteroidetes, and showed significant seasonal variation.					
36100751	7	80	theme	living	1061:1066	arg1	environment					1068:1078	the living environment	1057:1078	the living environment	1057:1078	were more abundant, and these were related to the living environment and feeding habits.					
36100751	0	81	from	study	12:16	arg1	deer					108:111	Forest musk deer	96:111	Forest musk deer	96:111	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	0	81	from	study	12:16	arg1	deer					87:90	Siberian musk deer	73:90	Siberian musk deer	73:90	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	6	82	from	related	898:904	arg1	FMD					949:951	FMD	949:951	FMD	949:951	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	0	83	theme	musk	82:85	arg1	deer					87:90	Siberian musk deer	73:90	Siberian musk deer	73:90	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	8	84	from	differences	1149:1159	arg1	functions					1196:1204	functions	1196:1204	functions	1196:1204	This indicates that FMD and SMD have significant differences in their microbial communities and functions.					
36100751	8	84	from	differences	1149:1159	arg1	communities					1180:1190	their microbial communities	1164:1190	their microbial communities	1164:1190	This indicates that FMD and SMD have significant differences in their microbial communities and functions.					
36100751	6	85	theme	enzymatic	873:881	arg1	reactions					883:891	The key enzymatic reactions	865:891	The key enzymatic reactions	865:891	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	6	85	theme	enzymatic	873:881	arg1	enzymes					954:960	enzymes	954:960	enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4	954:1007	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	6	85	theme	enzymatic	873:881	arg1	related					898:904	related	898:904	related	898:904	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	4	86	theme	corresponding	644:656	arg1	decrease					658:665	a corresponding decrease	642:665	a corresponding decrease in Bacteroidetes	642:682	The relative abundance of Firmicutes was significantly higher in SMD than in FMD, with a corresponding decrease in Bacteroidetes, and showed significant seasonal variation.					
36100751	3	87	theme	PCR	394:396	arg1	analysis					398:405	Quantitative PCR analysis	381:405	Quantitative PCR analysis	381:405	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	12	88	theme	musk	1756:1759	arg1	deer					1761:1764	musk deer	1756:1764	musk deer	1756:1764	This study is the first to analyze the composition of the gut microbiota of SMD and can be used to develop or modify conservation and husbandry strategies for musk deer, to improve their productivity.					
36100751	10	89	theme	seasonal	1328:1335	arg1	variations					1337:1346	seasonal variations	1328:1346	seasonal variations	1328:1346	For SMD, seasonal variations alter microbial communities and function.					
36100751	0	90	from	deer	108:111	arg1	study					12:16	Comparative study	0:16	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.	0:112	Comparative study of the function and structure of the gut microbiota in Siberian musk deer and Forest musk deer.					
36100751	6	91	dep	related	898:904	arg1	reactions					883:891	The key enzymatic reactions	865:891	The key enzymatic reactions	865:891	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	6	91	dep	related	898:904	arg1	enzymes					954:960	enzymes	954:960	enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4	954:1007	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	6	91	dep	related	898:904	arg1	related					898:904	related	898:904	related	898:904	The key enzymatic reactions were related to pyruvate metabolism in SMD; however, in FMD, enzymes that digest cellulose (EC:3.2.1.21, EC:3.2.1.4.)					
36100751	2	92	theme	musk	364:367	arg1	SMD					375:377	SMD	375:377	SMD	375:377	Considering the lack of a comprehensive understanding of the gut microbiota, there is a need to study the gut microbiota of Siberian musk deer (SMD).					
36100751	2	92	theme	musk	364:367	arg1	deer					369:372	Siberian musk deer	355:372	Siberian musk deer (SMD)	355:378	Considering the lack of a comprehensive understanding of the gut microbiota, there is a need to study the gut microbiota of Siberian musk deer (SMD).					
36100751	14	93	theme	relative	1933:1940	arg1	higher					1985:1990	higher	1985:1990	higher	1985:1990	• The energy metabolism and the relative abundance of Firmicutes were significantly higher in SMD.					
36100751	14	93	theme	relative	1933:1940	arg1	abundance					1942:1950	the relative abundance	1929:1950	the relative abundance of Firmicutes	1929:1964	• The energy metabolism and the relative abundance of Firmicutes were significantly higher in SMD.					
36100751	3	94	used	used	443:446	arg2	sequencing					427:436	high-throughput sequencing	411:436	high-throughput sequencing	411:436	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	3	94	used	used	443:446	arg2	analysis					398:405	Quantitative PCR analysis	381:405	Quantitative PCR analysis	381:405	Quantitative PCR analysis and high-throughput sequencing were used to show the differences in gut microbial communities and functions between SMD and forest musk deer (FMD).					
36100751	5	95	theme	energy	835:840	arg1	metabolism					842:851	energy metabolism	835:851	energy metabolism	835:851	The gut microbiome of FMD has enriched activity for carbohydrate metabolism, while in SMD, amino acids and energy metabolism was higher.					
36100751	11	96	theme	environmental	1571:1583	arg1	adaptation					1585:1594	environmental adaptation	1571:1594	environmental adaptation	1571:1594	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	2	97	theme	gut	337:339	arg1	microbiota					341:350	the gut microbiota	333:350	the gut microbiota of Siberian musk deer (SMD)	333:378	Considering the lack of a comprehensive understanding of the gut microbiota, there is a need to study the gut microbiota of Siberian musk deer (SMD).					
36100751	14	98	theme	energy	1907:1912	arg1	metabolism					1914:1923	The energy metabolism	1903:1923	The energy metabolism	1903:1923	• The energy metabolism and the relative abundance of Firmicutes were significantly higher in SMD.					
36100751	14	98	theme	energy	1907:1912	arg1	higher					1985:1990	higher	1985:1990	higher	1985:1990	• The energy metabolism and the relative abundance of Firmicutes were significantly higher in SMD.					
36100751	11	99	theme	selection	1557:1565	arg1	mechanism					1536:1544	winter-a mechanism	1527:1544	winter-a mechanism of natural selection and environmental adaptation	1527:1594	The key enzymes of the short-chain fatty acids (EC:1.3.1.44, EC:6.4.1.2) were significantly different, with higher relative abundance in winter-a mechanism of natural selection and environmental adaptation.					
36100751	4	100	theme	seasonal	708:715	arg1	variation					717:725	significant seasonal variation	696:725	significant seasonal variation	696:725	The relative abundance of Firmicutes was significantly higher in SMD than in FMD, with a corresponding decrease in Bacteroidetes, and showed significant seasonal variation.					
36100751	9	101	from	different	1277:1285	arg1	microbes					1294:1301	gut microbes	1290:1301	gut microbes of SMD and FMD	1290:1316	Furthermore, antibiotic resistances were identified and significantly different in gut microbes of SMD and FMD.					
36100751	13	102	theme	microbial	1839:1847	arg1	communities					1849:1859	microbial communities	1839:1859	microbial communities	1839:1859	KEY POINTS: • Significant differences in microbial communities and their function between FMD and SMD.					
36100751	2	103	theme	comprehensive	257:269	arg1	understanding					271:283	a comprehensive understanding	255:283	a comprehensive understanding of the gut microbiota	255:305	Considering the lack of a comprehensive understanding of the gut microbiota, there is a need to study the gut microbiota of Siberian musk deer (SMD).					
36100751	12	104	theme	gut	1655:1657	arg1	microbiota					1659:1668	the gut microbiota	1651:1668	the gut microbiota of SMD	1651:1675	This study is the first to analyze the composition of the gut microbiota of SMD and can be used to develop or modify conservation and husbandry strategies for musk deer, to improve their productivity.					
36100751	1	105	theme	artificial	186:195	arg1	breeding					197:204	artificial breeding	186:204	artificial breeding of musk deer	186:217	Musk deer are famous for their secretion of musk; however, the scale of artificial breeding of musk deer is limited.					
36461250	4	0	theme	original	846:853	arg1	approach					871:878	an original semi-industrial approach	843:878	an original semi-industrial approach	843:878	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	6	1	theme	different	1084:1092	arg1	ways					1094:1097	different ways	1084:1097	different ways	1084:1097	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	14	2	theme	aggregation	2210:2220	arg1	phase					2222:2226	the micelles aggregation phase	2197:2226	the micelles aggregation phase	2197:2226	An increase in protein denaturation disrupted the gel reorganization and led to the formation of weaker gels but did not interfere on the micelles aggregation phase and the early gelation.					
36461250	6	3	theme	solution	1247:1254	arg1	treatment					1138:1146	heat treatment	1133:1146	heat treatment of the 10 % w/w protein solution before vacuum evaporation	1133:1205	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	3	theme	solution	1247:1254	arg1	treatment					1213:1221	heat treatment	1208:1221	heat treatment of the 20 % w/w protein solution after vacuum concentration	1208:1281	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	3	4	theme	protein	514:520	arg1	concentrations					522:535	high protein concentrations	509:535	high protein concentrations	509:535	However, due to an incomplete understanding of phenomena taking place at high protein concentrations, shedding light on their mechanisms is a scientific challenge as well as an industrial need.					
36461250	10	5	from	effect	1580:1585	arg1	extent					1612:1617	the extent	1608:1617	the extent of protein denaturation	1608:1641	The effect of heat treatment on the extent of protein denaturation was not cumulative.					
36461250	6	6	theme	w/w	1160:1162	arg1	solution					1172:1179	the 10 % w/w protein solution	1151:1179	the 10 % w/w protein solution	1151:1179	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	7	theme	w/w	1235:1237	arg1	solution					1247:1254	the 20 % w/w protein solution	1226:1254	the 20 % w/w protein solution	1226:1254	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	13	8	theme	coagulation	1945:1955	arg1	properties					1957:1966	the enzymatic coagulation properties	1931:1966	the enzymatic coagulation properties of reconstituted protein powders	1931:1999	Our study showed that the enzymatic coagulation properties of reconstituted protein powders could be correlated with their physico-chemical compositions.					
36461250	1	9	theme	protein	129:135	arg1	contents					137:144	highly concentrated protein contents	109:144	highly concentrated protein contents	109:144	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	7	10	theme	10 	1396:1398	arg1	solution					1413:1420	unheated 10 % w/w protein solution	1387:1420	unheated 10 % w/w protein solution	1387:1420	A fourth powder was prepared from unheated 10 % w/w protein solution.					
36461250	5	11	dep	ultrafiltration	1006:1020	arg1	permeate					1022:1029	permeate	1022:1029	permeate	1022:1029	10 % w/w protein solutions constituted with whey protein and casein micelles at milk ratio, standardized in osmosed water or ultrafiltration permeate were used.					
36461250	6	12	theme	20 	1230:1232	arg1	%					1233:1233	%	1233:1233	%	1233:1233	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	2	13	theme	heat	285:288	arg1	step					309:312	a key step	303:312	a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities	303:433	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	2	13	theme	heat	285:288	arg1	treatment					290:298	heat treatment	285:298	heat treatment	285:298	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	4	14	from	influence	649:657	arg1	properties					814:823	enzymatic coagulation properties	792:823	enzymatic coagulation properties	792:823	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	4	14	from	influence	649:657	arg1	denaturation/aggregation					763:786	protein denaturation/aggregation	755:786	protein denaturation/aggregation	755:786	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	4	15	theme	heat	662:665	arg1	treatment					667:675	heat treatment	662:675	heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w)	662:750	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	4	15	theme	heat	662:665	arg1	30 s					685:688	74 °C/ 30 s	678:688	74 °C/ 30 s	678:688	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	3	16	theme	scientific	578:587	arg1	challenge					589:597	a scientific challenge	576:597	a scientific challenge as well as an industrial need	576:627	However, due to an incomplete understanding of phenomena taking place at high protein concentrations, shedding light on their mechanisms is a scientific challenge as well as an industrial need.					
36461250	3	16	theme	scientific	578:587	arg1	light					547:551	shedding light	538:551	shedding light on their mechanisms	538:571	However, due to an incomplete understanding of phenomena taking place at high protein concentrations, shedding light on their mechanisms is a scientific challenge as well as an industrial need.					
36461250	1	17	with	ingredients	92:102	arg1	contents					137:144	highly concentrated protein contents	109:144	highly concentrated protein contents	109:144	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	15	18	from	increase	2272:2279	arg1	strength					2290:2297	ionic strength	2284:2297	ionic strength	2284:2297	On the contrary, an increase in ionic strength and lactose content led to higher gel time.					
36461250	15	18	from	increase	2272:2279	arg1	content					2311:2317	lactose content	2303:2317	lactose content	2303:2317	On the contrary, an increase in ionic strength and lactose content led to higher gel time.					
36461250	6	19	theme	heat	1133:1136	arg1	treatment					1138:1146	heat treatment	1133:1146	heat treatment of the 10 % w/w protein solution before vacuum evaporation	1133:1205	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	19	theme	heat	1133:1136	arg1	treatments					1305:1314	two consecutive heat treatments	1284:1314	two consecutive heat treatments before and after vacuum evaporation	1284:1350	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	19	theme	heat	1133:1136	arg1	treatment					1213:1221	heat treatment	1208:1221	heat treatment of the 20 % w/w protein solution after vacuum concentration	1208:1281	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	14	20	theme	gels	2167:2170	arg1	formation					2147:2155	the formation	2143:2155	the formation of weaker gels	2143:2170	An increase in protein denaturation disrupted the gel reorganization and led to the formation of weaker gels but did not interfere on the micelles aggregation phase and the early gelation.					
36461250	5	21	used	used	1036:1039	arg2	ultrafiltration					1006:1020	ultrafiltration	1006:1020	ultrafiltration permeate	1006:1029	10 % w/w protein solutions constituted with whey protein and casein micelles at milk ratio, standardized in osmosed water or ultrafiltration permeate were used.					
36461250	2	22	theme	key	305:307	arg1	step					309:312	a key step	303:312	a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities	303:433	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	2	22	theme	key	305:307	arg1	treatment					290:298	heat treatment	285:298	heat treatment	285:298	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	7	23	theme	fourth	1355:1360	arg1	powder					1362:1367	A fourth powder	1353:1367	A fourth powder	1353:1367	A fourth powder was prepared from unheated 10 % w/w protein solution.					
36461250	6	24	theme	consecutive	1288:1298	arg1	treatment					1138:1146	heat treatment	1133:1146	heat treatment of the 10 % w/w protein solution before vacuum evaporation	1133:1205	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	24	theme	consecutive	1288:1298	arg1	treatments					1305:1314	two consecutive heat treatments	1284:1314	two consecutive heat treatments before and after vacuum evaporation	1284:1350	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	10	25	theme	heat	1590:1593	arg1	treatment					1595:1603	heat treatment	1590:1603	heat treatment	1590:1603	The effect of heat treatment on the extent of protein denaturation was not cumulative.					
36461250	15	26	theme	gel	2333:2335	arg1	time					2337:2340	higher gel time	2326:2340	higher gel time	2326:2340	On the contrary, an increase in ionic strength and lactose content led to higher gel time.					
36461250	6	27	theme	vacuum	1188:1193	arg1	evaporation					1195:1205	vacuum evaporation	1188:1205	vacuum evaporation	1188:1205	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	4	28	theme	concentrated	701:712	arg1	systems					727:733	highly concentrated milk protein systems	694:733	highly concentrated milk protein systems (up to 20 % w/w)	694:750	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	4	28	theme	concentrated	701:712	arg1	%					745:745	up to 20 % w/w	736:749	up to 20 % w/w	736:749	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	6	29	theme	vacuum	1262:1267	arg1	concentration					1269:1281	vacuum concentration	1262:1281	vacuum concentration	1262:1281	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	7	30	theme	w/w	1401:1403	arg1	solution					1413:1420	unheated 10 % w/w protein solution	1387:1420	unheated 10 % w/w protein solution	1387:1420	A fourth powder was prepared from unheated 10 % w/w protein solution.					
36461250	1	31	theme	high	150:153	arg1	products					167:174	high added value products	150:174	high added value products with expanding market	150:196	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	1	31	theme	high	150:153	arg1	ingredients					92:102	Dairy ingredients	86:102	Dairy ingredients with highly concentrated protein contents	86:144	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	6	32	theme	solution	1172:1179	arg1	treatment					1138:1146	heat treatment	1133:1146	heat treatment of the 10 % w/w protein solution before vacuum evaporation	1133:1205	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	32	theme	solution	1172:1179	arg1	treatments					1305:1314	two consecutive heat treatments	1284:1314	two consecutive heat treatments before and after vacuum evaporation	1284:1350	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	32	theme	solution	1172:1179	arg1	treatment					1213:1221	heat treatment	1208:1221	heat treatment of the 20 % w/w protein solution after vacuum concentration	1208:1281	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	1	33	theme	value	161:165	arg1	products					167:174	high added value products	150:174	high added value products with expanding market	150:196	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	1	33	theme	value	161:165	arg1	ingredients					92:102	Dairy ingredients	86:102	Dairy ingredients with highly concentrated protein contents	86:144	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	0	34	theme	coagulation	62:72	arg1	properties					74:83	enzymatic coagulation properties	52:83	enzymatic coagulation properties	52:83	Heat treatment of milk protein concentrates affects enzymatic coagulation properties.					
36461250	3	35	theme	industrial	613:622	arg1	need					624:627	an industrial need	610:627	a scientific challenge as well as an industrial need	576:627	However, due to an incomplete understanding of phenomena taking place at high protein concentrations, shedding light on their mechanisms is a scientific challenge as well as an industrial need.					
36461250	5	36	theme	10 	881:883	arg1	%					884:884	%	884:884	%	884:884	10 % w/w protein solutions constituted with whey protein and casein micelles at milk ratio, standardized in osmosed water or ultrafiltration permeate were used.					
36461250	3	37	from	light	547:551	arg1	mechanisms					562:571	their mechanisms	556:571	their mechanisms	556:571	However, due to an incomplete understanding of phenomena taking place at high protein concentrations, shedding light on their mechanisms is a scientific challenge as well as an industrial need.					
36461250	5	38	theme	w/w	886:888	arg1	solutions					898:906	10 % w/w protein solutions	881:906	10 % w/w protein solutions	881:906	10 % w/w protein solutions constituted with whey protein and casein micelles at milk ratio, standardized in osmosed water or ultrafiltration permeate were used.					
36461250	8	39	theme	higher	1469:1474	arg1	denaturation					1497:1508	a higher heat-induced protein denaturation	1467:1508	a higher heat-induced protein denaturation	1467:1508	An increase in protein concentration led to a higher heat-induced protein denaturation.					
36461250	5	40	dep	constituted	908:918	arg1	standardized					973:984	standardized	973:984	standardized in osmosed water	973:1001	10 % w/w protein solutions constituted with whey protein and casein micelles at milk ratio, standardized in osmosed water or ultrafiltration permeate were used.					
36461250	1	41	theme	concentrated	116:127	arg1	contents					137:144	highly concentrated protein contents	109:144	highly concentrated protein contents	109:144	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	6	42	theme	vacuum	1333:1338	arg1	evaporation					1340:1350	vacuum evaporation	1333:1350	vacuum evaporation	1333:1350	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	43	theme	%	1158:1158	arg1	solution					1172:1179	the 10 % w/w protein solution	1151:1179	the 10 % w/w protein solution	1151:1179	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	8	44	theme	protein	1489:1495	arg1	denaturation					1497:1508	a higher heat-induced protein denaturation	1467:1508	a higher heat-induced protein denaturation	1467:1508	An increase in protein concentration led to a higher heat-induced protein denaturation.					
36461250	14	45	theme	gel	2113:2115	arg1	reorganization					2117:2130	the gel reorganization	2109:2130	the gel reorganization	2109:2130	An increase in protein denaturation disrupted the gel reorganization and led to the formation of weaker gels but did not interfere on the micelles aggregation phase and the early gelation.					
36461250	10	46	theme	denaturation	1630:1641	arg1	extent					1612:1617	the extent	1608:1617	the extent of protein denaturation	1608:1641	The effect of heat treatment on the extent of protein denaturation was not cumulative.					
36461250	1	47	theme	Dairy	86:90	arg1	products					167:174	high added value products	150:174	high added value products with expanding market	150:196	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	1	47	theme	Dairy	86:90	arg1	ingredients					92:102	Dairy ingredients	86:102	Dairy ingredients with highly concentrated protein contents	86:144	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	4	48	theme	protein	719:725	arg1	systems					727:733	highly concentrated milk protein systems	694:733	highly concentrated milk protein systems (up to 20 % w/w)	694:750	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	4	48	theme	protein	719:725	arg1	%					745:745	up to 20 % w/w	736:749	up to 20 % w/w	736:749	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	2	49	theme	protein	379:385	arg1	structures					387:396	protein structures	379:396	protein structures	379:396	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	0	50	theme	Heat	0:3	arg1	treatment					5:13	Heat treatment	0:13	Heat treatment of milk protein concentrates	0:42	Heat treatment of milk protein concentrates affects enzymatic coagulation properties.					
36461250	12	51	theme	ionic	1873:1877	arg1	strength					1879:1886	low ionic strength	1869:1886	low ionic strength	1869:1886	This phenomenon was enhanced at low ionic strength and lactose content.					
36461250	6	52	theme	protein	1048:1054	arg1	solutions					1056:1064	These protein solutions	1042:1064	These protein solutions	1042:1064	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	14	53	theme	protein	2078:2084	arg1	denaturation					2086:2097	protein denaturation	2078:2097	protein denaturation	2078:2097	An increase in protein denaturation disrupted the gel reorganization and led to the formation of weaker gels but did not interfere on the micelles aggregation phase and the early gelation.					
36461250	6	54	dep	processed	1071:1079	arg1	treatment					1138:1146	heat treatment	1133:1146	heat treatment of the 10 % w/w protein solution before vacuum evaporation	1133:1205	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	54	dep	processed	1071:1079	arg1	treatments					1305:1314	two consecutive heat treatments	1284:1314	two consecutive heat treatments before and after vacuum evaporation	1284:1350	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	54	dep	processed	1071:1079	arg1	treatment					1213:1221	heat treatment	1208:1221	heat treatment of the 20 % w/w protein solution after vacuum concentration	1208:1281	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	55	theme	10 	1155:1157	arg1	%					1158:1158	%	1158:1158	%	1158:1158	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	14	56	from	increase	2066:2073	arg1	denaturation					2086:2097	protein denaturation	2078:2097	protein denaturation	2078:2097	An increase in protein denaturation disrupted the gel reorganization and led to the formation of weaker gels but did not interfere on the micelles aggregation phase and the early gelation.					
36461250	5	57	theme	casein	942:947	arg1	micelles					949:956	casein micelles	942:956	casein micelles	942:956	10 % w/w protein solutions constituted with whey protein and casein micelles at milk ratio, standardized in osmosed water or ultrafiltration permeate were used.					
36461250	14	58	theme	early	2236:2240	arg1	gelation					2242:2249	the early gelation	2232:2249	the early gelation	2232:2249	An increase in protein denaturation disrupted the gel reorganization and led to the formation of weaker gels but did not interfere on the micelles aggregation phase and the early gelation.					
36461250	0	59	theme	protein	23:29	arg1	concentrates					31:42	milk protein concentrates	18:42	milk protein concentrates	18:42	Heat treatment of milk protein concentrates affects enzymatic coagulation properties.					
36461250	11	60	theme	high	1666:1669	arg1	concentration					1679:1691	high protein concentration	1666:1691	high protein concentration	1666:1691	At high protein concentration, interactions between κ-casein and whey protein were modified compared to milk, as mainly micelle-bound aggregates were formed at pH about 6.7.					
36461250	4	61	theme	coagulation	802:812	arg1	properties					814:823	enzymatic coagulation properties	792:823	enzymatic coagulation properties	792:823	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	2	62	theme	unit	260:263	arg1	operations					265:274	unit operations	260:274	unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities	260:433	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	2	63	theme	such	218:221	arg1	ingredients					223:233	such ingredients	218:233	such ingredients	218:233	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	2	64	theme	microbial	331:339	arg1	safety					341:346	the microbial safety	327:346	the microbial safety	327:346	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	4	65	dep	20 	742:744	arg1	to					739:740	to	739:740	to	739:740	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	4	66	theme	protein	755:761	arg1	denaturation/aggregation					763:786	protein denaturation/aggregation	755:786	protein denaturation/aggregation	755:786	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	13	67	theme	reconstituted	1971:1983	arg1	powders					1993:1999	reconstituted protein powders	1971:1999	reconstituted protein powders	1971:1999	Our study showed that the enzymatic coagulation properties of reconstituted protein powders could be correlated with their physico-chemical compositions.					
36461250	5	68	theme	osmosed	989:995	arg1	water					997:1001	osmosed water	989:1001	osmosed water	989:1001	10 % w/w protein solutions constituted with whey protein and casein micelles at milk ratio, standardized in osmosed water or ultrafiltration permeate were used.					
36461250	13	69	theme	powders	1993:1999	arg1	properties					1957:1966	the enzymatic coagulation properties	1931:1966	the enzymatic coagulation properties of reconstituted protein powders	1931:1999	Our study showed that the enzymatic coagulation properties of reconstituted protein powders could be correlated with their physico-chemical compositions.					
36461250	3	70	theme	high	509:512	arg1	concentrations					522:535	high protein concentrations	509:535	high protein concentrations	509:535	However, due to an incomplete understanding of phenomena taking place at high protein concentrations, shedding light on their mechanisms is a scientific challenge as well as an industrial need.					
36461250	15	71	theme	ionic	2284:2288	arg1	strength					2290:2297	ionic strength	2284:2297	ionic strength	2284:2297	On the contrary, an increase in ionic strength and lactose content led to higher gel time.					
36461250	12	72	theme	lactose	1892:1898	arg1	content					1900:1906	lactose content	1892:1906	lactose content	1892:1906	This phenomenon was enhanced at low ionic strength and lactose content.					
36461250	14	73	theme	micelles	2201:2208	arg1	phase					2222:2226	the micelles aggregation phase	2197:2226	the micelles aggregation phase	2197:2226	An increase in protein denaturation disrupted the gel reorganization and led to the formation of weaker gels but did not interfere on the micelles aggregation phase and the early gelation.					
36461250	6	74	theme	protein	1239:1245	arg1	solution					1247:1254	the 20 % w/w protein solution	1226:1254	the 20 % w/w protein solution	1226:1254	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	13	75	theme	enzymatic	1935:1943	arg1	properties					1957:1966	the enzymatic coagulation properties	1931:1966	the enzymatic coagulation properties of reconstituted protein powders	1931:1999	Our study showed that the enzymatic coagulation properties of reconstituted protein powders could be correlated with their physico-chemical compositions.					
36461250	4	76	theme	semi-industrial	855:869	arg1	approach					871:878	an original semi-industrial approach	843:878	an original semi-industrial approach	843:878	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	7	77	theme	protein	1405:1411	arg1	solution					1413:1420	unheated 10 % w/w protein solution	1387:1420	unheated 10 % w/w protein solution	1387:1420	A fourth powder was prepared from unheated 10 % w/w protein solution.					
36461250	6	78	theme	%	1233:1233	arg1	solution					1247:1254	the 20 % w/w protein solution	1226:1254	the 20 % w/w protein solution	1226:1254	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	3	79	theme	shedding	538:545	arg1	challenge					589:597	a scientific challenge	576:597	a scientific challenge as well as an industrial need	576:627	However, due to an incomplete understanding of phenomena taking place at high protein concentrations, shedding light on their mechanisms is a scientific challenge as well as an industrial need.					
36461250	3	79	theme	shedding	538:545	arg1	light					547:551	shedding light	538:551	shedding light on their mechanisms	538:571	However, due to an incomplete understanding of phenomena taking place at high protein concentrations, shedding light on their mechanisms is a scientific challenge as well as an industrial need.					
36461250	1	80	theme	expanding	181:189	arg1	market					191:196	expanding market	181:196	expanding market	181:196	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	7	81	theme	%	1399:1399	arg1	solution					1413:1420	unheated 10 % w/w protein solution	1387:1420	unheated 10 % w/w protein solution	1387:1420	A fourth powder was prepared from unheated 10 % w/w protein solution.					
36461250	2	82	from	changes	368:374	arg1	structures					387:396	protein structures	379:396	protein structures	379:396	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	7	83	theme	unheated	1387:1394	arg1	solution					1413:1420	unheated 10 % w/w protein solution	1387:1420	unheated 10 % w/w protein solution	1387:1420	A fourth powder was prepared from unheated 10 % w/w protein solution.					
36461250	11	84	theme	micelle-bound	1783:1795	arg1	aggregates					1797:1806	micelle-bound aggregates	1783:1806	micelle-bound aggregates	1783:1806	At high protein concentration, interactions between κ-casein and whey protein were modified compared to milk, as mainly micelle-bound aggregates were formed at pH about 6.7.					
36461250	14	85	theme	weaker	2160:2165	arg1	gels					2167:2170	weaker gels	2160:2170	weaker gels	2160:2170	An increase in protein denaturation disrupted the gel reorganization and led to the formation of weaker gels but did not interfere on the micelles aggregation phase and the early gelation.					
36461250	6	86	theme	heat	1300:1303	arg1	treatment					1138:1146	heat treatment	1133:1146	heat treatment of the 10 % w/w protein solution before vacuum evaporation	1133:1205	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	86	theme	heat	1300:1303	arg1	treatments					1305:1314	two consecutive heat treatments	1284:1314	two consecutive heat treatments before and after vacuum evaporation	1284:1350	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	87	theme	heat	1208:1211	arg1	treatment					1138:1146	heat treatment	1133:1146	heat treatment of the 10 % w/w protein solution before vacuum evaporation	1133:1205	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	6	87	theme	heat	1208:1211	arg1	treatment					1213:1221	heat treatment	1208:1221	heat treatment of the 20 % w/w protein solution after vacuum concentration	1208:1281	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	10	88	theme	treatment	1595:1603	arg1	effect					1580:1585	The effect	1576:1585	The effect of heat treatment on the extent of protein denaturation	1576:1641	The effect of heat treatment on the extent of protein denaturation was not cumulative.					
36461250	5	89	theme	protein	890:896	arg1	solutions					898:906	10 % w/w protein solutions	881:906	10 % w/w protein solutions	881:906	10 % w/w protein solutions constituted with whey protein and casein micelles at milk ratio, standardized in osmosed water or ultrafiltration permeate were used.					
36461250	8	90	theme	protein	1438:1444	arg1	concentration					1446:1458	protein concentration	1438:1458	protein concentration	1438:1458	An increase in protein concentration led to a higher heat-induced protein denaturation.					
36461250	3	91	theme	phenomena	483:491	arg1	understanding					466:478	an incomplete understanding	452:478	an incomplete understanding of phenomena taking place at high protein concentrations	452:535	However, due to an incomplete understanding of phenomena taking place at high protein concentrations, shedding light on their mechanisms is a scientific challenge as well as an industrial need.					
36461250	8	92	from	increase	1426:1433	arg1	concentration					1446:1458	protein concentration	1438:1458	protein concentration	1438:1458	An increase in protein concentration led to a higher heat-induced protein denaturation.					
36461250	15	93	theme	higher	2326:2331	arg1	time					2337:2340	higher gel time	2326:2340	higher gel time	2326:2340	On the contrary, an increase in ionic strength and lactose content led to higher gel time.					
36461250	2	94	theme	operations	265:274	arg1	succession					246:255	a succession	244:255	a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities	244:433	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	2	94	theme	operations	265:274	arg1	step					309:312	a key step	303:312	a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities	303:433	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	2	94	theme	operations	265:274	arg1	treatment					290:298	heat treatment	285:298	heat treatment	285:298	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	1	95	theme	added	155:159	arg1	products					167:174	high added value products	150:174	high added value products with expanding market	150:196	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	1	95	theme	added	155:159	arg1	ingredients					92:102	Dairy ingredients	86:102	Dairy ingredients with highly concentrated protein contents	86:144	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	6	96	theme	protein	1164:1170	arg1	solution					1172:1179	the 10 % w/w protein solution	1151:1179	the 10 % w/w protein solution	1151:1179	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	4	97	theme	treatment	667:675	arg1	influence					649:657	the influence	645:657	the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties	645:823	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	11	98	theme	whey	1728:1731	arg1	protein					1733:1739	whey protein	1728:1739	whey protein	1728:1739	At high protein concentration, interactions between κ-casein and whey protein were modified compared to milk, as mainly micelle-bound aggregates were formed at pH about 6.7.					
36461250	1	99	with	products	167:174	arg1	market					191:196	expanding market	181:196	expanding market	181:196	Dairy ingredients with highly concentrated protein contents are high added value products with expanding market.					
36461250	5	100	theme	%	884:884	arg1	solutions					898:906	10 % w/w protein solutions	881:906	10 % w/w protein solutions	881:906	10 % w/w protein solutions constituted with whey protein and casein micelles at milk ratio, standardized in osmosed water or ultrafiltration permeate were used.					
36461250	8	101	theme	heat-induced	1476:1487	arg1	denaturation					1497:1508	a higher heat-induced protein denaturation	1467:1508	a higher heat-induced protein denaturation	1467:1508	An increase in protein concentration led to a higher heat-induced protein denaturation.					
36461250	13	102	theme	physico-chemical	2032:2047	arg1	compositions					2049:2060	their physico-chemical compositions	2026:2060	their physico-chemical compositions	2026:2060	Our study showed that the enzymatic coagulation properties of reconstituted protein powders could be correlated with their physico-chemical compositions.					
36461250	12	103	theme	low	1869:1871	arg1	strength					1879:1886	low ionic strength	1869:1886	low ionic strength	1869:1886	This phenomenon was enhanced at low ionic strength and lactose content.					
36461250	5	104	theme	milk	961:964	arg1	ratio					966:970	milk ratio	961:970	milk ratio	961:970	10 % w/w protein solutions constituted with whey protein and casein micelles at milk ratio, standardized in osmosed water or ultrafiltration permeate were used.					
36461250	10	105	theme	protein	1622:1628	arg1	denaturation					1630:1641	protein denaturation	1622:1641	protein denaturation	1622:1641	The effect of heat treatment on the extent of protein denaturation was not cumulative.					
36461250	5	106	theme	whey	925:928	arg1	protein					930:936	whey protein	925:936	whey protein	925:936	10 % w/w protein solutions constituted with whey protein and casein micelles at milk ratio, standardized in osmosed water or ultrafiltration permeate were used.					
36461250	4	107	theme	milk	714:717	arg1	systems					727:733	highly concentrated milk protein systems	694:733	highly concentrated milk protein systems (up to 20 % w/w)	694:750	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	4	107	theme	milk	714:717	arg1	%					745:745	up to 20 % w/w	736:749	up to 20 % w/w	736:749	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	0	108	theme	milk	18:21	arg1	concentrates					31:42	milk protein concentrates	18:42	milk protein concentrates	18:42	Heat treatment of milk protein concentrates affects enzymatic coagulation properties.					
36461250	9	109	theme	lactose	1559:1565	arg1	content					1567:1573	the lactose content	1555:1573	the lactose content	1555:1573	This phenomenon was reduced when increasing the lactose content.					
36461250	11	110	theme	protein	1671:1677	arg1	concentration					1679:1691	high protein concentration	1666:1691	high protein concentration	1666:1691	At high protein concentration, interactions between κ-casein and whey protein were modified compared to milk, as mainly micelle-bound aggregates were formed at pH about 6.7.					
36461250	4	111	theme	systems	727:733	arg1	treatment					667:675	heat treatment	662:675	heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w)	662:750	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	4	111	theme	systems	727:733	arg1	30 s					685:688	74 °C/ 30 s	678:688	74 °C/ 30 s	678:688	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	0	112	theme	concentrates	31:42	arg1	treatment					5:13	Heat treatment	0:13	Heat treatment of milk protein concentrates	0:42	Heat treatment of milk protein concentrates affects enzymatic coagulation properties.					
36461250	3	113	theme	incomplete	455:464	arg1	understanding					466:478	an incomplete understanding	452:478	an incomplete understanding of phenomena taking place at high protein concentrations	452:535	However, due to an incomplete understanding of phenomena taking place at high protein concentrations, shedding light on their mechanisms is a scientific challenge as well as an industrial need.					
36461250	0	114	theme	enzymatic	52:60	arg1	properties					74:83	enzymatic coagulation properties	52:83	enzymatic coagulation properties	52:83	Heat treatment of milk protein concentrates affects enzymatic coagulation properties.					
36461250	6	115	theme	powders	1124:1130	arg1	manufacture					1109:1119	the manufacture	1105:1119	the manufacture of powders	1105:1130	These protein solutions were processed in different ways prior the manufacture of powders: heat treatment of the 10 % w/w protein solution before vacuum evaporation, heat treatment of the 20 % w/w protein solution after vacuum concentration, two consecutive heat treatments before and after vacuum evaporation.					
36461250	2	116	theme	ingredients	223:233	arg1	manufacture					203:213	The manufacture	199:213	The manufacture of such ingredients	199:233	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	4	117	theme	enzymatic	792:800	arg1	properties					814:823	enzymatic coagulation properties	792:823	enzymatic coagulation properties	792:823	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	4	118	theme	w/w	747:749	arg1	systems					727:733	highly concentrated milk protein systems	694:733	highly concentrated milk protein systems (up to 20 % w/w)	694:750	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	4	118	theme	w/w	747:749	arg1	%					745:745	up to 20 % w/w	736:749	up to 20 % w/w	736:749	In this study, the influence of heat treatment (74 °C/ 30 s) of highly concentrated milk protein systems (up to 20 % w/w) on protein denaturation/aggregation and enzymatic coagulation properties was studied using an original semi-industrial approach.					
36461250	13	119	theme	protein	1985:1991	arg1	powders					1993:1999	reconstituted protein powders	1971:1999	reconstituted protein powders	1971:1999	Our study showed that the enzymatic coagulation properties of reconstituted protein powders could be correlated with their physico-chemical compositions.					
36461250	2	120	theme	major	362:366	arg1	changes					368:374	major changes	362:374	major changes in protein structures	362:396	The manufacture of such ingredients includes a succession of unit operations of which heat treatment is a key step to guarantee the microbial safety, that induces major changes in protein structures and thus ingredients functionalities.					
36461250	15	121	theme	lactose	2303:2309	arg1	content					2311:2317	lactose content	2303:2317	lactose content	2303:2317	On the contrary, an increase in ionic strength and lactose content led to higher gel time.					
35866189	4	0	theme	GelMA	846:850	arg1	combination					831:841	the combination	827:841	the combination of GelMA and HAMA	827:859	The composition of collagen and glycosaminoglycan in native skin was recapitulated by adjusting the combination of GelMA and HAMA.					
35866189	3	1	theme	printed	536:542	arg1	equivalents					549:559	3D printed skin equivalents	533:559	3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers	533:636	In this study, 3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers were fabricated using gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink.					
35866189	0	2	theme	Epidermal-Papillary-Dermal	66:91	arg1	Layers					93:98	Epidermal-Papillary-Dermal Layers	66:98	Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels	66:138	3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.					
35866189	7	3	theme	skin	1265:1268	arg1	engineering					1277:1287	skin tissue engineering	1265:1287	skin tissue engineering	1265:1287	Furthermore, they may serve as useful models for skin tissue engineering and regeneration.					
35866189	3	4	theme	skin	544:547	arg1	equivalents					549:559	3D printed skin equivalents	533:559	3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers	533:636	In this study, 3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers were fabricated using gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink.					
35866189	6	5	theme	promising	1143:1151	arg1	hydrogels					1129:1137	GelMA/HAMA hydrogels	1118:1137	GelMA/HAMA hydrogels	1118:1137	The results indicate that GelMA/HAMA hydrogels are promising candidates as bioinks for the 3D printing of skin equivalents.					
35866189	6	5	theme	promising	1143:1151	arg1	candidates					1153:1162	promising candidates	1143:1162	promising candidates as bioinks for the 3D printing of skin equivalents	1143:1213	The results indicate that GelMA/HAMA hydrogels are promising candidates as bioinks for the 3D printing of skin equivalents.					
35866189	0	6	theme	Gelatin/Hyaluronic	106:123	arg1	Hydrogels					130:138	Gelatin/Hyaluronic Acid Hydrogels	106:138	Gelatin/Hyaluronic Acid Hydrogels	106:138	3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.					
35866189	7	7	theme	useful	1247:1252	arg1	models					1254:1259	useful models	1247:1259	useful models for skin tissue engineering and regeneration	1247:1304	Furthermore, they may serve as useful models for skin tissue engineering and regeneration.					
35866189	7	7	theme	useful	1247:1252	arg1	they					1229:1232	they	1229:1232	they	1229:1232	Furthermore, they may serve as useful models for skin tissue engineering and regeneration.					
35866189	4	8	theme	HAMA	856:859	arg1	combination					831:841	the combination	827:841	the combination of GelMA and HAMA	827:859	The composition of collagen and glycosaminoglycan in native skin was recapitulated by adjusting the combination of GelMA and HAMA.					
35866189	5	9	theme	pore	1074:1077	arg1	development					1079:1089	spontaneous hair pore development	1057:1089	spontaneous hair pore development	1057:1089	The GelMA/HAMA bioink exhibited excellent viscoelastic and physicochemical properties, 3D printability, cytocompatibility, and functionality to maintain hair-inductive potency while facilitating spontaneous hair pore development.					
35866189	5	10	theme	hair-inductive	1015:1028	arg1	potency					1030:1036	hair-inductive potency	1015:1036	hair-inductive potency	1015:1036	The GelMA/HAMA bioink exhibited excellent viscoelastic and physicochemical properties, 3D printability, cytocompatibility, and functionality to maintain hair-inductive potency while facilitating spontaneous hair pore development.					
35866189	5	11	theme	hair	1069:1072	arg1	development					1079:1089	spontaneous hair pore development	1057:1089	spontaneous hair pore development	1057:1089	The GelMA/HAMA bioink exhibited excellent viscoelastic and physicochemical properties, 3D printability, cytocompatibility, and functionality to maintain hair-inductive potency while facilitating spontaneous hair pore development.					
35866189	4	12	theme	native	784:789	arg1	skin					791:794	native skin	784:794	native skin	784:794	The composition of collagen and glycosaminoglycan in native skin was recapitulated by adjusting the combination of GelMA and HAMA.					
35866189	3	13	theme	hair	575:578	arg1	follicle					580:587	hair follicle	575:587	hair follicle structures	575:598	In this study, 3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers were fabricated using gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink.					
35866189	1	14	theme	bioprinting	183:193	arg1	technologies					195:206	three-dimensional (3D) bioprinting technologies	160:206	three-dimensional (3D) bioprinting technologies	160:206	Recent advances in three-dimensional (3D) bioprinting technologies have enabled the fabrication of sophisticated live 3D tissue analogs.					
35866189	0	15	theme	Acid	125:128	arg1	Hydrogels					130:138	Gelatin/Hyaluronic Acid Hydrogels	106:138	Gelatin/Hyaluronic Acid Hydrogels	106:138	3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.					
35866189	1	16	from	advances	148:155	arg1	technologies					195:206	three-dimensional (3D) bioprinting technologies	160:206	three-dimensional (3D) bioprinting technologies	160:206	Recent advances in three-dimensional (3D) bioprinting technologies have enabled the fabrication of sophisticated live 3D tissue analogs.					
35866189	0	17	theme	3D	0:1	arg1	Printing					3:10	3D Printing	0:10	3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.	0:139	3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.					
35866189	2	18	theme	extracellular	426:438	arg1	matrix					440:445	a native extracellular matrix	417:445	a native extracellular matrix	417:445	Despite the existing hydrogel-based bioinks, the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells remains challenging.					
35866189	2	19	theme	cells	491:495	arg1	properties					471:480	the intrinsic properties	457:480	the intrinsic properties of laden cells	457:495	Despite the existing hydrogel-based bioinks, the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells remains challenging.					
35866189	2	20	theme	bioink	351:356	arg1	materials					358:366	advanced bioink materials	342:366	advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells	342:495	Despite the existing hydrogel-based bioinks, the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells remains challenging.					
35866189	4	21	from	composition	735:745	arg1	skin					791:794	native skin	784:794	native skin	784:794	The composition of collagen and glycosaminoglycan in native skin was recapitulated by adjusting the combination of GelMA and HAMA.					
35866189	2	22	theme	native	419:424	arg1	matrix					440:445	a native extracellular matrix	417:445	a native extracellular matrix	417:445	Despite the existing hydrogel-based bioinks, the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells remains challenging.					
35866189	2	23	theme	laden	485:489	arg1	cells					491:495	laden cells	485:495	laden cells	485:495	Despite the existing hydrogel-based bioinks, the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells remains challenging.					
35866189	6	24	theme	GelMA/HAMA	1118:1127	arg1	hydrogels					1129:1137	GelMA/HAMA hydrogels	1118:1137	GelMA/HAMA hydrogels	1118:1137	The results indicate that GelMA/HAMA hydrogels are promising candidates as bioinks for the 3D printing of skin equivalents.					
35866189	6	24	theme	GelMA/HAMA	1118:1127	arg1	candidates					1153:1162	promising candidates	1143:1162	promising candidates as bioinks for the 3D printing of skin equivalents	1143:1213	The results indicate that GelMA/HAMA hydrogels are promising candidates as bioinks for the 3D printing of skin equivalents.					
35866189	2	25	theme	advanced	342:349	arg1	materials					358:366	advanced bioink materials	342:366	advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells	342:495	Despite the existing hydrogel-based bioinks, the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells remains challenging.					
35866189	4	26	theme	collagen	750:757	arg1	composition					735:745	The composition	731:745	The composition of collagen and glycosaminoglycan in native skin	731:794	The composition of collagen and glycosaminoglycan in native skin was recapitulated by adjusting the combination of GelMA and HAMA.					
35866189	0	27	theme	Skin	15:18	arg1	Equivalents					20:30	Skin Equivalents	15:30	Skin Equivalents	15:30	3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.					
35866189	1	28	dep	three-dimensional	160:176	arg1	3D					179:180	3D	179:180	3D	179:180	Recent advances in three-dimensional (3D) bioprinting technologies have enabled the fabrication of sophisticated live 3D tissue analogs.					
35866189	2	29	theme	intrinsic	461:469	arg1	properties					471:480	the intrinsic properties	457:480	the intrinsic properties of laden cells	457:495	Despite the existing hydrogel-based bioinks, the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells remains challenging.					
35866189	3	30	theme	dermal	624:629	arg1	layers					631:636	epidermal/papillary dermal layers	604:636	epidermal/papillary dermal layers	604:636	In this study, 3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers were fabricated using gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink.					
35866189	5	31	theme	3D	949:950	arg1	printability					952:963	3D printability	949:963	3D printability	949:963	The GelMA/HAMA bioink exhibited excellent viscoelastic and physicochemical properties, 3D printability, cytocompatibility, and functionality to maintain hair-inductive potency while facilitating spontaneous hair pore development.					
35866189	5	31	theme	3D	949:950	arg1	viscoelastic					904:915	viscoelastic	904:915	viscoelastic	904:915	The GelMA/HAMA bioink exhibited excellent viscoelastic and physicochemical properties, 3D printability, cytocompatibility, and functionality to maintain hair-inductive potency while facilitating spontaneous hair pore development.					
35866189	5	32	theme	GelMA/HAMA	866:875	arg1	bioink					877:882	The GelMA/HAMA bioink	862:882	The GelMA/HAMA bioink	862:882	The GelMA/HAMA bioink exhibited excellent viscoelastic and physicochemical properties, 3D printability, cytocompatibility, and functionality to maintain hair-inductive potency while facilitating spontaneous hair pore development.					
35866189	3	33	theme	follicle	580:587	arg1	structures					589:598	hair follicle structures	575:598	hair follicle structures	575:598	In this study, 3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers were fabricated using gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink.					
35866189	3	34	theme	gelatin	660:666	arg1	bioink					723:728	gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink	660:728	gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink	660:728	In this study, 3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers were fabricated using gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink.					
35866189	6	35	theme	equivalents	1203:1213	arg1	printing					1186:1193	the 3D printing	1179:1193	the 3D printing of skin equivalents	1179:1213	The results indicate that GelMA/HAMA hydrogels are promising candidates as bioinks for the 3D printing of skin equivalents.					
35866189	0	36	theme	Equivalents	20:30	arg1	Printing					3:10	3D Printing	0:10	3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.	0:139	3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.					
35866189	3	37	theme	methacryloyl/hyaluronic	668:690	arg1	bioink					723:728	gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink	660:728	gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink	660:728	In this study, 3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers were fabricated using gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink.					
35866189	1	38	theme	sophisticated	240:252	arg1	analogs					269:275	sophisticated live 3D tissue analogs	240:275	sophisticated live 3D tissue analogs	240:275	Recent advances in three-dimensional (3D) bioprinting technologies have enabled the fabrication of sophisticated live 3D tissue analogs.					
35866189	0	39	theme	Follicle	42:49	arg1	Structures					51:60	Hair Follicle Structures	37:60	Hair Follicle Structures	37:60	3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.					
35866189	0	40	theme	Hair	37:40	arg1	Structures					51:60	Hair Follicle Structures	37:60	Hair Follicle Structures	37:60	3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.					
35866189	2	41	theme	matrix	440:445	arg1	composition					402:412	the composition	398:412	the composition of a native extracellular matrix	398:445	Despite the existing hydrogel-based bioinks, the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells remains challenging.					
35866189	5	42	theme	physicochemical	921:935	arg1	properties					937:946	excellent viscoelastic and physicochemical properties	894:946	properties	937:946	The GelMA/HAMA bioink exhibited excellent viscoelastic and physicochemical properties, 3D printability, cytocompatibility, and functionality to maintain hair-inductive potency while facilitating spontaneous hair pore development.					
35866189	0	43	with	Printing	3:10	arg1	Structures					51:60	Hair Follicle Structures	37:60	Hair Follicle Structures	37:60	3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.					
35866189	0	43	with	Printing	3:10	arg1	Layers					93:98	Epidermal-Papillary-Dermal Layers	66:98	Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels	66:138	3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.					
35866189	1	44	theme	Recent	141:146	arg1	advances					148:155	Recent advances	141:155	Recent advances in three-dimensional (3D) bioprinting technologies	141:206	Recent advances in three-dimensional (3D) bioprinting technologies have enabled the fabrication of sophisticated live 3D tissue analogs.					
35866189	2	45	theme	existing	290:297	arg1	bioinks					314:320	the existing hydrogel-based bioinks	286:320	the existing hydrogel-based bioinks	286:320	Despite the existing hydrogel-based bioinks, the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells remains challenging.					
35866189	5	46	theme	spontaneous	1057:1067	arg1	development					1079:1089	spontaneous hair pore development	1057:1089	spontaneous hair pore development	1057:1089	The GelMA/HAMA bioink exhibited excellent viscoelastic and physicochemical properties, 3D printability, cytocompatibility, and functionality to maintain hair-inductive potency while facilitating spontaneous hair pore development.					
35866189	3	47	theme	epidermal/papillary	604:622	arg1	layers					631:636	epidermal/papillary dermal layers	604:636	epidermal/papillary dermal layers	604:636	In this study, 3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers were fabricated using gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink.					
35866189	1	48	theme	live	254:257	arg1	analogs					269:275	sophisticated live 3D tissue analogs	240:275	sophisticated live 3D tissue analogs	240:275	Recent advances in three-dimensional (3D) bioprinting technologies have enabled the fabrication of sophisticated live 3D tissue analogs.					
35866189	2	49	theme	hydrogel-based	299:312	arg1	bioinks					314:320	the existing hydrogel-based bioinks	286:320	the existing hydrogel-based bioinks	286:320	Despite the existing hydrogel-based bioinks, the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells remains challenging.					
35866189	3	50	theme	GelMA/HAMA	711:720	arg1	bioink					723:728	gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink	660:728	gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink	660:728	In this study, 3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers were fabricated using gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink.					
35866189	2	51	theme	materials	358:366	arg1	development					327:337	the development	323:337	the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells	323:495	Despite the existing hydrogel-based bioinks, the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells remains challenging.					
35866189	1	52	theme	3D	259:260	arg1	analogs					269:275	sophisticated live 3D tissue analogs	240:275	sophisticated live 3D tissue analogs	240:275	Recent advances in three-dimensional (3D) bioprinting technologies have enabled the fabrication of sophisticated live 3D tissue analogs.					
35866189	6	53	theme	3D	1183:1184	arg1	printing					1186:1193	the 3D printing	1179:1193	the 3D printing of skin equivalents	1179:1213	The results indicate that GelMA/HAMA hydrogels are promising candidates as bioinks for the 3D printing of skin equivalents.					
35866189	3	54	theme	acid	692:695	arg1	bioink					723:728	gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink	660:728	gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink	660:728	In this study, 3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers were fabricated using gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink.					
35866189	6	55	theme	skin	1198:1201	arg1	equivalents					1203:1213	skin equivalents	1198:1213	skin equivalents	1198:1213	The results indicate that GelMA/HAMA hydrogels are promising candidates as bioinks for the 3D printing of skin equivalents.					
35866189	1	56	theme	three-dimensional	160:176	arg1	technologies					195:206	three-dimensional (3D) bioprinting technologies	160:206	three-dimensional (3D) bioprinting technologies	160:206	Recent advances in three-dimensional (3D) bioprinting technologies have enabled the fabrication of sophisticated live 3D tissue analogs.					
35866189	1	57	theme	tissue	262:267	arg1	analogs					269:275	sophisticated live 3D tissue analogs	240:275	sophisticated live 3D tissue analogs	240:275	Recent advances in three-dimensional (3D) bioprinting technologies have enabled the fabrication of sophisticated live 3D tissue analogs.					
35866189	7	58	theme	tissue	1270:1275	arg1	engineering					1277:1287	skin tissue engineering	1265:1287	skin tissue engineering	1265:1287	Furthermore, they may serve as useful models for skin tissue engineering and regeneration.					
35866189	3	59	theme	methacryloyl	697:708	arg1	bioink					723:728	gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink	660:728	gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink	660:728	In this study, 3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers were fabricated using gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink.					
35866189	4	60	theme	glycosaminoglycan	763:779	arg1	composition					735:745	The composition	731:745	The composition of collagen and glycosaminoglycan in native skin	731:794	The composition of collagen and glycosaminoglycan in native skin was recapitulated by adjusting the combination of GelMA and HAMA.					
35866189	1	61	theme	analogs	269:275	arg1	fabrication					225:235	the fabrication	221:235	the fabrication of sophisticated live 3D tissue analogs	221:275	Recent advances in three-dimensional (3D) bioprinting technologies have enabled the fabrication of sophisticated live 3D tissue analogs.					
35635092	4	0	theme	flexible	891:898	arg1	films					900:904	the flexible films	887:904	the flexible films	887:904	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
35635092	6	1	theme	applied	1087:1093	arg1	force					1106:1110	the applied mechanical force	1083:1110	the applied mechanical force	1083:1110	In addition, the charge density shows a proportional relation with the applied mechanical force, and it could sense stress of 1 kPa.					
35635092	3	2	theme	flexible	721:728	arg1	films					730:734	The flexible films	717:734	The flexible films made using the polarization process	717:770	The flexible films made using the polarization process are tested.					
35635092	9	3	theme	curve	1370:1374	arg1	analysis					1354:1361	analysis	1354:1361	analysis of the curve	1354:1374	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	2	4	theme	zirconate	615:623	arg1	powder					634:639	lead zirconate titanate powder	610:639	lead zirconate titanate powder	610:639	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	10	5	theme	injury	1697:1702	arg1	detection					1704:1712	sports injury detection	1690:1712	sports injury detection	1690:1712	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	1	6	theme	healthcare	283:292	arg1	monitoring					294:303	healthcare monitoring	283:303	healthcare monitoring	283:303	Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility.					
35635092	4	7	theme	tensile	870:876	arg1	force					878:882	the maximum tensile force	858:882	the maximum tensile force of the flexible films	858:904	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
35635092	4	7	theme	tensile	870:876	arg1	N					913:913	26 N	910:913	26 N	910:913	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
35635092	7	8	with	connection	1239:1248	arg1	module					1269:1274	the detection module	1255:1274	the detection module	1255:1274	Finally, plantar pressure sensors are arranged and packaged with a film array followed by connection with the detection module.					
35635092	1	9	theme	great	207:211	arg1	promise					213:219	great promise	207:219	great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility	207:426	Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility.					
35635092	2	10	theme	microfibrillated	645:660	arg1	PZT/MFC					673:679	PZT/MFC	673:679	PZT/MFC	673:679	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	2	10	theme	microfibrillated	645:660	arg1	cellulose					662:670	microfibrillated cellulose	645:670	microfibrillated cellulose (PZT/MFC)	645:680	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	10	11	theme	rehabilitation	1726:1739	arg1	training					1741:1748	medical rehabilitation training	1718:1748	medical rehabilitation training	1718:1748	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	9	12	theme	body	1405:1408	arg1	motions					1410:1416	human body motions	1399:1416	human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury	1399:1513	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	2	13	theme	novel	582:586	arg1	films					595:599	novel hybrid films	582:599	novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements	582:714	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	0	14	theme	health	142:147	arg1	monitoring					149:158	human health monitoring	136:158	human health monitoring	136:158	A plantar wearable pressure sensor based on hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring.					
35635092	1	15	theme	Flexible	161:168	arg1	sensors					194:200	Flexible and wearable electronic sensors	161:200	Flexible and wearable electronic sensors	161:200	Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility.					
35635092	2	16	theme	flexible	527:534	arg1	requirements					536:547	the flexible requirements	523:547	the flexible requirements for wearable sensors	523:568	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	2	17	theme	high	440:443	arg1	performance					459:469	high piezoelectric performance	440:469	high piezoelectric performance similar to that of rigid materials	440:504	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	0	18	theme	lead	51:54	arg1	films					126:130	hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films	44:130	hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring	44:158	A plantar wearable pressure sensor based on hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring.					
35635092	9	19	theme	patients	1471:1478	arg1	rehabilitation					1444:1457	the rehabilitation	1440:1457	the rehabilitation of diabetic patients	1440:1478	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	9	19	theme	patients	1471:1478	arg1	detection					1488:1496	the detection	1484:1496	the detection of sports injury	1484:1513	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	0	20	theme	cellulose	92:100	arg1	films					126:130	hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films	44:130	hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring	44:158	A plantar wearable pressure sensor based on hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring.					
35635092	1	21	theme	high	344:347	arg1	coupling					367:374	high electromechanical coupling	344:374	high electromechanical coupling performance	344:386	Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility.					
35635092	2	22	theme	lead	610:613	arg1	powder					634:639	lead zirconate titanate powder	610:639	lead zirconate titanate powder	610:639	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	9	23	from	detection	1488:1496	arg1	important					1427:1435	important	1427:1435	important	1427:1435	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	0	24	theme	composite	116:124	arg1	films					126:130	hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films	44:130	hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring	44:158	A plantar wearable pressure sensor based on hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring.					
35635092	1	25	theme	coupling	367:374	arg1	performance					376:386	high electromechanical coupling performance	344:386	high electromechanical coupling performance	344:386	Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility.					
35635092	5	26	theme	bending	932:938	arg1	angles					940:945	bending angles	932:945	bending angles between 15° and 180°	932:966	A wide range of bending angles between 15° and 180° proves the flexibility capability of the films.					
35635092	4	27	theme	maximum	806:812	arg1	N-1					850:852	31 pC N-1	844:852	31 pC N-1	844:852	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
35635092	4	27	theme	maximum	806:812	arg1	coefficient					828:838	the maximum piezoelectric coefficient	802:838	the maximum piezoelectric coefficient	802:838	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
35635092	10	28	theme	proposed	1592:1599	arg1	sensor					1635:1640	the proposed PZT/MFC wearable plantar pressure sensor	1588:1640	the proposed PZT/MFC wearable plantar pressure sensor	1588:1640	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	7	29	theme	pressure	1166:1173	arg1	sensors					1175:1181	plantar pressure sensors	1158:1181	plantar pressure sensors	1158:1181	Finally, plantar pressure sensors are arranged and packaged with a film array followed by connection with the detection module.					
35635092	8	30	from	curves	1296:1301	arg1	plantar					1324:1330	the plantar	1320:1330	the plantar	1320:1330	Then, the pressure curves of each point on the plantar are obtained.					
35635092	9	31	theme	injury	1508:1513	arg1	rehabilitation					1444:1457	the rehabilitation	1440:1457	the rehabilitation of diabetic patients	1440:1478	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	9	31	theme	injury	1508:1513	arg1	detection					1488:1496	the detection	1484:1496	the detection of sports injury	1484:1513	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	1	32	theme	monitoring	294:303	arg1	field					274:278	the field	270:278	the field of healthcare monitoring	270:303	Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility.					
35635092	2	33	theme	pressure	694:701	arg1	measurements					703:714	plantar pressure measurements	686:714	plantar pressure measurements	686:714	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	10	34	theme	pressure	1626:1633	arg1	sensor					1635:1640	the proposed PZT/MFC wearable plantar pressure sensor	1588:1640	the proposed PZT/MFC wearable plantar pressure sensor	1588:1640	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	0	35	theme	wearable	10:17	arg1	sensor					28:33	A plantar wearable pressure sensor	0:33	A plantar wearable pressure sensor	0:33	A plantar wearable pressure sensor based on hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring.					
35635092	9	36	theme	several	1377:1383	arg1	parameters					1385:1394	several parameters	1377:1394	several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury	1377:1513	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	6	37	theme	charge	1033:1038	arg1	density					1040:1046	the charge density	1029:1046	the charge density	1029:1046	In addition, the charge density shows a proportional relation with the applied mechanical force, and it could sense stress of 1 kPa.					
35635092	10	38	theme	wearable	1609:1616	arg1	sensor					1635:1640	the proposed PZT/MFC wearable plantar pressure sensor	1588:1640	the proposed PZT/MFC wearable plantar pressure sensor	1588:1640	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	9	39	theme	stride	1543:1548	arg1	frequency					1550:1558	stride frequency	1543:1558	stride frequency	1543:1558	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	10	40	theme	application	1652:1662	arg1	prospects					1664:1672	broad application prospects	1646:1672	broad application prospects	1646:1672	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	1	41	theme	life	250:253	arg1	quality					239:245	the quality	235:245	the quality of life	235:253	Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility.					
35635092	1	42	theme	wearable	174:181	arg1	sensors					194:200	Flexible and wearable electronic sensors	161:200	Flexible and wearable electronic sensors	161:200	Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility.					
35635092	5	43	theme	films	1009:1013	arg1	capability					991:1000	the flexibility capability	975:1000	the flexibility capability of the films	975:1013	A wide range of bending angles between 15° and 180° proves the flexibility capability of the films.					
35635092	6	44	theme	mechanical	1095:1104	arg1	force					1106:1110	the applied mechanical force	1083:1110	the applied mechanical force	1083:1110	In addition, the charge density shows a proportional relation with the applied mechanical force, and it could sense stress of 1 kPa.					
35635092	2	45	theme	titanate	625:632	arg1	powder					634:639	lead zirconate titanate powder	610:639	lead zirconate titanate powder	610:639	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	7	46	theme	film	1216:1219	arg1	array					1221:1225	a film array	1214:1225	a film array followed by connection with the detection module	1214:1274	Finally, plantar pressure sensors are arranged and packaged with a film array followed by connection with the detection module.					
35635092	10	47	theme	detection	1704:1712	arg1	field					1681:1685	the field	1677:1685	the field of sports injury detection and medical rehabilitation training	1677:1748	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	4	48	theme	films	900:904	arg1	force					878:882	the maximum tensile force	858:882	the maximum tensile force of the flexible films	858:904	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
35635092	4	48	theme	films	900:904	arg1	N					913:913	26 N	910:913	26 N	910:913	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
35635092	10	49	theme	sports	1690:1695	arg1	detection					1704:1712	sports injury detection	1690:1712	sports injury detection	1690:1712	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	4	50	theme	maximum	862:868	arg1	force					878:882	the maximum tensile force	858:882	the maximum tensile force of the flexible films	858:904	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
35635092	4	50	theme	maximum	862:868	arg1	N					913:913	26 N	910:913	26 N	910:913	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
35635092	9	51	from	rehabilitation	1444:1457	arg1	important					1427:1435	important	1427:1435	important	1427:1435	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	6	52	theme	kPa	1144:1146	arg1	stress					1132:1137	stress	1132:1137	stress of 1 kPa	1132:1146	In addition, the charge density shows a proportional relation with the applied mechanical force, and it could sense stress of 1 kPa.					
35635092	3	53	theme	polarization	751:762	arg1	process					764:770	the polarization process	747:770	the polarization process	747:770	The flexible films made using the polarization process are tested.					
35635092	10	54	theme	training	1741:1748	arg1	field					1681:1685	the field	1677:1685	the field of sports injury detection and medical rehabilitation training	1677:1748	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	9	55	theme	human	1399:1403	arg1	motions					1410:1416	human body motions	1399:1416	human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury	1399:1513	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	10	56	theme	medical	1718:1724	arg1	training					1741:1748	medical rehabilitation training	1718:1748	medical rehabilitation training	1718:1748	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	9	57	theme	motions	1410:1416	arg1	parameters					1385:1394	several parameters	1377:1394	several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury	1377:1513	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	10	58	contain	has	1642:1644	arg1	sensor					1635:1640	the proposed PZT/MFC wearable plantar pressure sensor	1588:1640	the proposed PZT/MFC wearable plantar pressure sensor	1588:1640	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	10	58	contain	has	1642:1644	arg2	prospects					1664:1672	broad application prospects	1646:1672	broad application prospects	1646:1672	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	5	59	theme	wide	918:921	arg1	range					923:927	A wide range	916:927	A wide range of bending angles between 15° and 180°	916:966	A wide range of bending angles between 15° and 180° proves the flexibility capability of the films.					
35635092	2	60	theme	hybrid	588:593	arg1	films					595:599	novel hybrid films	582:599	novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements	582:714	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	0	61	theme	zirconate-titanate/microfibrillated	56:90	arg1	films					126:130	hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films	44:130	hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring	44:158	A plantar wearable pressure sensor based on hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring.					
35635092	9	62	theme	diabetic	1462:1469	arg1	patients					1471:1478	diabetic patients	1462:1478	diabetic patients	1462:1478	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	0	63	theme	piezoelectric	102:114	arg1	films					126:130	hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films	44:130	hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring	44:158	A plantar wearable pressure sensor based on hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring.					
35635092	1	64	theme	electronic	183:192	arg1	sensors					194:200	Flexible and wearable electronic sensors	161:200	Flexible and wearable electronic sensors	161:200	Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility.					
35635092	2	65	theme	plantar	686:692	arg1	measurements					703:714	plantar pressure measurements	686:714	plantar pressure measurements	686:714	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	8	66	theme	pressure	1287:1294	arg1	curves					1296:1301	the pressure curves	1283:1301	the pressure curves of each point on the plantar	1283:1330	Then, the pressure curves of each point on the plantar are obtained.					
35635092	1	67	theme	electromechanical	349:365	arg1	coupling					367:374	high electromechanical coupling	344:374	high electromechanical coupling performance	344:386	Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility.					
35635092	2	68	theme	wearable	553:560	arg1	sensors					562:568	wearable sensors	553:568	wearable sensors	553:568	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	0	69	theme	human	136:140	arg1	monitoring					149:158	human health monitoring	136:158	human health monitoring	136:158	A plantar wearable pressure sensor based on hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring.					
35635092	9	70	theme	sports	1501:1506	arg1	injury					1508:1513	sports injury	1501:1513	sports injury	1501:1513	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	8	71	theme	point	1311:1315	arg1	curves					1296:1301	the pressure curves	1283:1301	the pressure curves of each point on the plantar	1283:1330	Then, the pressure curves of each point on the plantar are obtained.					
35635092	7	72	theme	plantar	1158:1164	arg1	sensors					1175:1181	plantar pressure sensors	1158:1181	plantar pressure sensors	1158:1181	Finally, plantar pressure sensors are arranged and packaged with a film array followed by connection with the detection module.					
35635092	0	73	theme	plantar	2:8	arg1	sensor					28:33	A plantar wearable pressure sensor	0:33	A plantar wearable pressure sensor	0:33	A plantar wearable pressure sensor based on hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring.					
35635092	1	74	theme	high	389:392	arg1	sensitivity					394:404	high sensitivity	389:404	high sensitivity	389:404	Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility.					
35635092	5	75	theme	angles	940:945	arg1	range					923:927	A wide range	916:927	A wide range of bending angles between 15° and 180°	916:966	A wide range of bending angles between 15° and 180° proves the flexibility capability of the films.					
35635092	6	76	theme	proportional	1056:1067	arg1	relation					1069:1076	a proportional relation	1054:1076	a proportional relation with the applied mechanical force	1054:1110	In addition, the charge density shows a proportional relation with the applied mechanical force, and it could sense stress of 1 kPa.					
35635092	2	77	theme	similar	471:477	arg1	performance					459:469	high piezoelectric performance	440:469	high piezoelectric performance similar to that of rigid materials	440:504	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	0	78	theme	pressure	19:26	arg1	sensor					28:33	A plantar wearable pressure sensor	0:33	A plantar wearable pressure sensor	0:33	A plantar wearable pressure sensor based on hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring.					
35635092	2	79	theme	piezoelectric	445:457	arg1	performance					459:469	high piezoelectric performance	440:469	high piezoelectric performance similar to that of rigid materials	440:504	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	10	80	theme	plantar	1618:1624	arg1	sensor					1635:1640	the proposed PZT/MFC wearable plantar pressure sensor	1588:1640	the proposed PZT/MFC wearable plantar pressure sensor	1588:1640	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	7	81	theme	detection	1259:1267	arg1	module					1269:1274	the detection module	1255:1274	the detection module	1255:1274	Finally, plantar pressure sensors are arranged and packaged with a film array followed by connection with the detection module.					
35635092	2	82	theme	rigid	490:494	arg1	materials					496:504	rigid materials	490:504	rigid materials	490:504	To achieve high piezoelectric performance similar to that of rigid materials while satisfying the flexible requirements for wearable sensors, we propose novel hybrid films based on lead zirconate titanate powder and microfibrillated cellulose (PZT/MFC) for plantar pressure measurements.					
35635092	4	83	theme	pC	847:848	arg1	N-1					850:852	31 pC N-1	844:852	31 pC N-1	844:852	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
35635092	4	83	theme	pC	847:848	arg1	coefficient					828:838	the maximum piezoelectric coefficient	802:838	the maximum piezoelectric coefficient	802:838	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
35635092	10	84	theme	PZT/MFC	1601:1607	arg1	sensor					1635:1640	the proposed PZT/MFC wearable plantar pressure sensor	1588:1640	the proposed PZT/MFC wearable plantar pressure sensor	1588:1640	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	0	85	theme	hybrid	44:49	arg1	films					126:130	hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films	44:130	hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring	44:158	A plantar wearable pressure sensor based on hybrid lead zirconate-titanate/microfibrillated cellulose piezoelectric composite films for human health monitoring.					
35635092	1	86	theme	low	321:323	arg1	cost					325:328	their low cost	315:328	their low cost	315:328	Flexible and wearable electronic sensors hold great promise for improving the quality of life, especially in the field of healthcare monitoring, owing to their low cost, flexibility, high electromechanical coupling performance, high sensitivity, and biocompatibility.					
35635092	6	87	with	relation	1069:1076	arg1	force					1106:1110	the applied mechanical force	1083:1110	the applied mechanical force	1083:1110	In addition, the charge density shows a proportional relation with the applied mechanical force, and it could sense stress of 1 kPa.					
35635092	9	88	from	important	1427:1435	arg1	rehabilitation					1444:1457	the rehabilitation	1440:1457	the rehabilitation of diabetic patients	1440:1478	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	9	88	from	important	1427:1435	arg1	detection					1488:1496	the detection	1484:1496	the detection of sports injury	1484:1513	Through analysis of the curve, several parameters of human body motions that are important in the rehabilitation of diabetic patients and the detection of sports injury can be performed, including stride frequency, length and speed.					
35635092	10	89	theme	broad	1646:1650	arg1	prospects					1664:1672	broad application prospects	1646:1672	broad application prospects	1646:1672	Overall, the proposed PZT/MFC wearable plantar pressure sensor has broad application prospects in the field of sports injury detection and medical rehabilitation training.					
35635092	5	90	theme	flexibility	979:989	arg1	capability					991:1000	the flexibility capability	975:1000	the flexibility capability of the films	975:1013	A wide range of bending angles between 15° and 180° proves the flexibility capability of the films.					
35635092	4	91	theme	piezoelectric	814:826	arg1	N-1					850:852	31 pC N-1	844:852	31 pC N-1	844:852	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
35635092	4	91	theme	piezoelectric	814:826	arg1	coefficient					828:838	the maximum piezoelectric coefficient	802:838	the maximum piezoelectric coefficient	802:838	It was found that the maximum piezoelectric coefficient was 31 pC N-1 and the maximum tensile force of the flexible films was 26 N.					
36382954	4	0	theme	mucosal	945:951	arg1	responses					973:981	enhanced mucosal and systemic immune responses	936:981	enhanced mucosal and systemic immune responses	936:981	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	3	1	theme	trimethyl	565:573	arg1	chloride					584:591	N-2-hydroxypropyl trimethyl ammonium chloride	547:591	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	547:602	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	6	2	theme	adjuvant	1562:1569	arg1	system					1524:1529	the chitosan-based nano-delivery system	1491:1529	the chitosan-based nano-delivery system	1491:1529	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	2	theme	adjuvant	1562:1569	arg1	promise					1591:1597	great promise	1585:1597	great promise in viral infectious diseases and immunization therapy	1585:1651	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	2	theme	adjuvant	1562:1569	arg1	vaccine					1554:1560	the mucosal vaccine adjuvant	1542:1569	the mucosal vaccine adjuvant	1542:1569	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	4	3	theme	systemic	957:964	arg1	responses					973:981	enhanced mucosal and systemic immune responses	936:981	enhanced mucosal and systemic immune responses	936:981	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	4	theme	N-2-HACC/CMCS	1113:1125	arg1	vaccine					1136:1142	a BSA-loaded N-2-HACC/CMCS adjuvant vaccine	1100:1142	a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs)	1100:1166	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	4	theme	N-2-HACC/CMCS	1113:1125	arg1	NPs					1163:1165	N-2-HACC/CMCS/BSA NPs	1145:1165	N-2-HACC/CMCS/BSA NPs	1145:1165	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	3	5	theme	chitosan	620:627	arg1	nanoparticles					629:641	O-carboxymethyl chitosan nanoparticles	604:641	O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption	604:695	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	3	5	theme	chitosan	620:627	arg1	NPs					658:660	N-2-HACC/CMCS NPs	644:660	N-2-HACC/CMCS NPs	644:660	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	3	6	theme	cross-linking	783:795	arg1	method					797:802	the ionic cross-linking method	773:802	the ionic cross-linking method	773:802	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	3	7	with	responses	746:754	arg1	absorption					686:695	excellent mucosal absorption	668:695	excellent mucosal absorption	668:695	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	1	8	theme	novel	187:191	arg1	approach					229:236	a novel non-invasive vaccine administration approach	185:236	a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration	185:366	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	1	8	theme	novel	187:191	arg1	administration					146:159	Nasal administration	140:159	Nasal administration for vaccine delivery	140:180	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	2	9	with	effects	475:481	arg1	ability					447:453	sustained-release ability	429:453	sustained-release ability	429:453	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	6	10	theme	immunization	1632:1643	arg1	therapy					1645:1651	immunization therapy	1632:1651	immunization therapy	1632:1651	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	1	11	theme	vaccine	206:212	arg1	approach					229:236	a novel non-invasive vaccine administration approach	185:236	a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration	185:366	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	1	11	theme	vaccine	206:212	arg1	administration					146:159	Nasal administration	140:159	Nasal administration for vaccine delivery	140:180	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	3	12	theme	mucosal	678:684	arg1	absorption					686:695	excellent mucosal absorption	668:695	excellent mucosal absorption	668:695	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	4	13	theme	responses	973:981	arg1	induction					923:931	the induction	919:931	the induction of enhanced mucosal and systemic immune responses	919:981	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	14	theme	bovine	984:989	arg1	albumin					997:1003	bovine serum albumin	984:1003	bovine serum albumin (BSA)	984:1009	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	14	theme	bovine	984:989	arg1	antigen					1043:1049	a general model antigen	1027:1049	a general model antigen	1027:1049	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	14	theme	bovine	984:989	arg1	BSA					1006:1008	BSA	1006:1008	BSA	1006:1008	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	6	15	theme	mucosal	1546:1552	arg1	system					1524:1529	the chitosan-based nano-delivery system	1491:1529	the chitosan-based nano-delivery system	1491:1529	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	15	theme	mucosal	1546:1552	arg1	promise					1591:1597	great promise	1585:1597	great promise in viral infectious diseases and immunization therapy	1585:1651	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	15	theme	mucosal	1546:1552	arg1	vaccine					1554:1560	the mucosal vaccine adjuvant	1542:1569	the mucosal vaccine adjuvant	1542:1569	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	5	16	theme	factors	1377:1383	arg1	secretion					1339:1347	the secretion	1335:1347	the secretion of related pro-inflammatory factors	1335:1383	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	5	16	theme	factors	1377:1383	arg1	proliferation					1302:1314	the proliferation	1298:1314	the proliferation of lymphocytes	1298:1329	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	3	17	theme	drug	703:706	arg1	capacity					716:723	high drug loading capacity	698:723	high drug loading capacity	698:723	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	4	18	theme	general	1029:1035	arg1	albumin					997:1003	bovine serum albumin	984:1003	bovine serum albumin (BSA)	984:1009	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	18	theme	general	1029:1035	arg1	antigen					1043:1049	a general model antigen	1027:1049	a general model antigen	1027:1049	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	0	19	theme	Composite	69:77	arg1	Nanoparticles					79:91	Functionalized Chitosan-Based Composite Nanoparticles	39:91	Functionalized Chitosan-Based Composite Nanoparticles as a Vaccine Adjuvant for Intranasal Delivery	39:137	Enhanced Immune Responses to Mucosa by Functionalized Chitosan-Based Composite Nanoparticles as a Vaccine Adjuvant for Intranasal Delivery.					
36382954	1	20	theme	vaccine	165:171	arg1	delivery					173:180	vaccine delivery	165:180	vaccine delivery	165:180	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	5	21	theme	related	1352:1358	arg1	factors					1377:1383	related pro-inflammatory factors	1352:1383	related pro-inflammatory factors	1352:1383	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	4	22	theme	NPs	861:863	arg1	vaccine					870:876	a vaccine adjuvant	868:885	a vaccine adjuvant	868:885	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	22	theme	NPs	861:863	arg1	capacity					831:838	the potential capacity	817:838	the potential capacity of the N-2-HACC/CMCS NPs	817:863	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	22	theme	NPs	861:863	arg1	mechanism					905:913	the molecular mechanism	891:913	the molecular mechanism for the induction of enhanced mucosal and systemic immune responses	891:981	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	5	23	theme	mucosal	1427:1433	arg1	responses					1455:1463	specific mucosal and systemic immune responses	1418:1463	specific mucosal and systemic immune responses	1418:1463	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	5	24	theme	systemic	1439:1446	arg1	responses					1455:1463	specific mucosal and systemic immune responses	1418:1463	specific mucosal and systemic immune responses	1418:1463	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	2	25	theme	sustained-release	429:445	arg1	ability					447:453	sustained-release ability	429:453	sustained-release ability	429:453	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	5	26	theme	mucosal	1253:1259	arg1	absorption					1261:1270	mucosal absorption	1253:1270	mucosal absorption	1253:1270	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	2	27	theme	immune	468:473	arg1	effects					475:481	enhanced immune effects	459:481	enhanced immune effects at mucosal sites	459:498	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	1	28	theme	traditional	330:340	arg1	administration					353:366	traditional injectable administration	330:366	traditional injectable administration	330:366	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	2	29	with	vaccine	386:392	arg1	ability					447:453	sustained-release ability	429:453	sustained-release ability	429:453	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	5	30	theme	N-2-HACC/CMCS/BSA	1203:1219	arg1	NPs					1221:1223	the N-2-HACC/CMCS/BSA NPs	1199:1223	the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption	1199:1270	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	6	31	theme	chitosan-based	1495:1508	arg1	system					1524:1529	the chitosan-based nano-delivery system	1491:1529	the chitosan-based nano-delivery system	1491:1529	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	31	theme	chitosan-based	1495:1508	arg1	promise					1591:1597	great promise	1585:1597	great promise in viral infectious diseases and immunization therapy	1585:1651	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	31	theme	chitosan-based	1495:1508	arg1	vaccine					1554:1560	the mucosal vaccine adjuvant	1542:1569	the mucosal vaccine adjuvant	1542:1569	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	32	contain	possesses	1575:1583	arg1	system					1524:1529	the chitosan-based nano-delivery system	1491:1529	the chitosan-based nano-delivery system	1491:1529	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	32	contain	possesses	1575:1583	arg2	promise					1591:1597	great promise	1585:1597	great promise in viral infectious diseases and immunization therapy	1585:1651	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	32	contain	possesses	1575:1583	arg2	system					1524:1529	the chitosan-based nano-delivery system	1491:1529	the chitosan-based nano-delivery system	1491:1529	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	32	contain	possesses	1575:1583	arg1	vaccine					1554:1560	the mucosal vaccine adjuvant	1542:1569	the mucosal vaccine adjuvant	1542:1569	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	32	contain	possesses	1575:1583	arg1	promise					1591:1597	great promise	1585:1597	great promise in viral infectious diseases and immunization therapy	1585:1651	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	32	contain	possesses	1575:1583	arg2	vaccine					1554:1560	the mucosal vaccine adjuvant	1542:1569	the mucosal vaccine adjuvant	1542:1569	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	2	33	with	systems	416:422	arg1	ability					447:453	sustained-release ability	429:453	sustained-release ability	429:453	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	3	34	theme	immune	739:744	arg1	responses					746:754	enhanced immune responses	730:754	enhanced immune responses	730:754	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	4	35	theme	adjuvant	878:885	arg1	vaccine					870:876	a vaccine adjuvant	868:885	a vaccine adjuvant	868:885	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	35	theme	adjuvant	878:885	arg1	capacity					831:838	the potential capacity	817:838	the potential capacity of the N-2-HACC/CMCS NPs	817:863	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	5	36	theme	responses	1455:1463	arg1	stimulation					1403:1413	the stimulation	1399:1413	the stimulation of specific mucosal and systemic immune responses	1399:1463	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	3	37	theme	ammonium	575:582	arg1	chloride					584:591	N-2-hydroxypropyl trimethyl ammonium chloride	547:591	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	547:602	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	6	38	theme	great	1585:1589	arg1	system					1524:1529	the chitosan-based nano-delivery system	1491:1529	the chitosan-based nano-delivery system	1491:1529	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	38	theme	great	1585:1589	arg1	promise					1591:1597	great promise	1585:1597	great promise in viral infectious diseases and immunization therapy	1585:1651	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	38	theme	great	1585:1589	arg1	vaccine					1554:1560	the mucosal vaccine adjuvant	1542:1569	the mucosal vaccine adjuvant	1542:1569	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	5	39	theme	lymphocytes	1319:1329	arg1	secretion					1339:1347	the secretion	1335:1347	the secretion of related pro-inflammatory factors	1335:1383	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	5	39	theme	lymphocytes	1319:1329	arg1	proliferation					1302:1314	the proliferation	1298:1314	the proliferation of lymphocytes	1298:1329	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	6	40	theme	nano-delivery	1510:1522	arg1	system					1524:1529	the chitosan-based nano-delivery system	1491:1529	the chitosan-based nano-delivery system	1491:1529	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	40	theme	nano-delivery	1510:1522	arg1	promise					1591:1597	great promise	1585:1597	great promise in viral infectious diseases and immunization therapy	1585:1651	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	40	theme	nano-delivery	1510:1522	arg1	vaccine					1554:1560	the mucosal vaccine adjuvant	1542:1569	the mucosal vaccine adjuvant	1542:1569	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	4	41	theme	BSA-loaded	1102:1111	arg1	vaccine					1136:1142	a BSA-loaded N-2-HACC/CMCS adjuvant vaccine	1100:1142	a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs)	1100:1166	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	41	theme	BSA-loaded	1102:1111	arg1	NPs					1163:1165	N-2-HACC/CMCS/BSA NPs	1145:1165	N-2-HACC/CMCS/BSA NPs	1145:1165	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	3	42	theme	ionic	777:781	arg1	method					797:802	the ionic cross-linking method	773:802	the ionic cross-linking method	773:802	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	2	43	theme	adjuvant	398:405	arg1	systems					416:422	mucosal vaccine and adjuvant delivery systems	378:422	systems	416:422	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	1	44	theme	immune	272:277	arg1	responses					279:287	local or systemic immune responses	254:287	local or systemic immune responses	254:287	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	4	45	theme	enhanced	936:943	arg1	responses					973:981	enhanced mucosal and systemic immune responses	936:981	enhanced mucosal and systemic immune responses	936:981	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	46	theme	adjuvant	1127:1134	arg1	vaccine					1136:1142	a BSA-loaded N-2-HACC/CMCS adjuvant vaccine	1100:1142	a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs)	1100:1166	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	46	theme	adjuvant	1127:1134	arg1	NPs					1163:1165	N-2-HACC/CMCS/BSA NPs	1145:1165	N-2-HACC/CMCS/BSA NPs	1145:1165	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	3	47	theme	O-carboxymethyl	604:618	arg1	nanoparticles					629:641	O-carboxymethyl chitosan nanoparticles	604:641	O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption	604:695	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	3	47	theme	O-carboxymethyl	604:618	arg1	NPs					658:660	N-2-HACC/CMCS NPs	644:660	N-2-HACC/CMCS NPs	644:660	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	5	48	with	NPs	1221:1223	arg1	biostability					1236:1247	great biostability	1230:1247	great biostability	1230:1247	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	5	48	with	NPs	1221:1223	arg1	absorption					1261:1270	mucosal absorption	1253:1270	mucosal absorption	1253:1270	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	0	49	theme	Functionalized	39:52	arg1	Nanoparticles					79:91	Functionalized Chitosan-Based Composite Nanoparticles	39:91	Functionalized Chitosan-Based Composite Nanoparticles as a Vaccine Adjuvant for Intranasal Delivery	39:137	Enhanced Immune Responses to Mucosa by Functionalized Chitosan-Based Composite Nanoparticles as a Vaccine Adjuvant for Intranasal Delivery.					
36382954	3	50	theme	N-2-HACC/CMCS	644:656	arg1	nanoparticles					629:641	O-carboxymethyl chitosan nanoparticles	604:641	O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption	604:695	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	3	50	theme	N-2-HACC/CMCS	644:656	arg1	NPs					658:660	N-2-HACC/CMCS NPs	644:660	N-2-HACC/CMCS NPs	644:660	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	1	51	theme	non-invasive	193:204	arg1	approach					229:236	a novel non-invasive vaccine administration approach	185:236	a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration	185:366	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	1	51	theme	non-invasive	193:204	arg1	administration					146:159	Nasal administration	140:159	Nasal administration for vaccine delivery	140:180	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	3	52	with	capacity	716:723	arg1	absorption					686:695	excellent mucosal absorption	668:695	excellent mucosal absorption	668:695	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	1	53	theme	administration	214:227	arg1	approach					229:236	a novel non-invasive vaccine administration approach	185:236	a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration	185:366	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	1	53	theme	administration	214:227	arg1	administration					146:159	Nasal administration	140:159	Nasal administration for vaccine delivery	140:180	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	4	54	theme	serum	991:995	arg1	albumin					997:1003	bovine serum albumin	984:1003	bovine serum albumin (BSA)	984:1009	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	54	theme	serum	991:995	arg1	antigen					1043:1049	a general model antigen	1027:1049	a general model antigen	1027:1049	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	54	theme	serum	991:995	arg1	BSA					1006:1008	BSA	1006:1008	BSA	1006:1008	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	3	55	theme	N-2-hydroxypropyl	547:563	arg1	chloride					584:591	N-2-hydroxypropyl trimethyl ammonium chloride	547:591	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	547:602	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	3	56	theme	excellent	668:676	arg1	absorption					686:695	excellent mucosal absorption	668:695	excellent mucosal absorption	668:695	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	6	57	theme	infectious	1608:1617	arg1	diseases					1619:1626	viral infectious diseases	1602:1626	viral infectious diseases	1602:1626	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	4	58	theme	immune	966:971	arg1	responses					973:981	enhanced mucosal and systemic immune responses	936:981	enhanced mucosal and systemic immune responses	936:981	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	5	59	theme	pro-inflammatory	1360:1375	arg1	factors					1377:1383	related pro-inflammatory factors	1352:1383	related pro-inflammatory factors	1352:1383	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	3	60	theme	high	698:701	arg1	capacity					716:723	high drug loading capacity	698:723	high drug loading capacity	698:723	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	1	61	theme	Nasal	140:144	arg1	approach					229:236	a novel non-invasive vaccine administration approach	185:236	a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration	185:366	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	1	61	theme	Nasal	140:144	arg1	administration					146:159	Nasal administration	140:159	Nasal administration for vaccine delivery	140:180	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	2	62	from	sites	494:498	arg1	vaccine					386:392	mucosal vaccine and adjuvant delivery systems	378:422	vaccine	386:392	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	2	62	from	sites	494:498	arg1	effects					475:481	enhanced immune effects	459:481	enhanced immune effects at mucosal sites	459:498	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	2	62	from	sites	494:498	arg1	systems					416:422	mucosal vaccine and adjuvant delivery systems	378:422	systems	416:422	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	4	63	theme	model	1037:1041	arg1	albumin					997:1003	bovine serum albumin	984:1003	bovine serum albumin (BSA)	984:1009	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	63	theme	model	1037:1041	arg1	antigen					1043:1049	a general model antigen	1027:1049	a general model antigen	1027:1049	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	0	64	theme	Adjuvant	106:113	arg1	Vaccine					98:104	a Vaccine	96:104	a Vaccine Adjuvant for Intranasal Delivery	96:137	Enhanced Immune Responses to Mucosa by Functionalized Chitosan-Based Composite Nanoparticles as a Vaccine Adjuvant for Intranasal Delivery.					
36382954	5	65	theme	specific	1418:1425	arg1	responses					1455:1463	specific mucosal and systemic immune responses	1418:1463	specific mucosal and systemic immune responses	1418:1463	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	0	66	theme	Intranasal	119:128	arg1	Delivery					130:137	Intranasal Delivery	119:137	Intranasal Delivery	119:137	Enhanced Immune Responses to Mucosa by Functionalized Chitosan-Based Composite Nanoparticles as a Vaccine Adjuvant for Intranasal Delivery.					
36382954	4	67	theme	N-2-HACC/CMCS	847:859	arg1	NPs					861:863	the N-2-HACC/CMCS NPs	843:863	the N-2-HACC/CMCS NPs	843:863	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	0	68	theme	Immune	9:14	arg1	Responses					16:24	Immune Responses	9:24	Immune Responses to Mucosa	9:34	Enhanced Immune Responses to Mucosa by Functionalized Chitosan-Based Composite Nanoparticles as a Vaccine Adjuvant for Intranasal Delivery.					
36382954	4	69	theme	N-2-HACC/CMCS	1071:1083	arg1	NPs					1085:1087	the N-2-HACC/CMCS NPs	1067:1087	the N-2-HACC/CMCS NPs	1067:1087	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	2	70	theme	mucosal	486:492	arg1	sites					494:498	mucosal sites	486:498	mucosal sites	486:498	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	4	71	theme	potential	821:829	arg1	vaccine					870:876	a vaccine adjuvant	868:885	a vaccine adjuvant	868:885	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	71	theme	potential	821:829	arg1	capacity					831:838	the potential capacity	817:838	the potential capacity of the N-2-HACC/CMCS NPs	817:863	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	71	theme	potential	821:829	arg1	mechanism					905:913	the molecular mechanism	891:913	the molecular mechanism for the induction of enhanced mucosal and systemic immune responses	891:981	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	3	72	with	chitosan/N	593:602	arg1	absorption					686:695	excellent mucosal absorption	668:695	excellent mucosal absorption	668:695	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	6	73	from	promise	1591:1597	arg1	diseases					1619:1626	viral infectious diseases	1602:1626	viral infectious diseases	1602:1626	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	6	73	from	promise	1591:1597	arg1	therapy					1645:1651	immunization therapy	1632:1651	immunization therapy	1632:1651	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	3	74	theme	loading	708:714	arg1	capacity					716:723	high drug loading capacity	698:723	high drug loading capacity	698:723	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	2	75	theme	enhanced	459:466	arg1	effects					475:481	enhanced immune effects	459:481	enhanced immune effects at mucosal sites	459:498	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	0	76	theme	Chitosan-Based	54:67	arg1	Nanoparticles					79:91	Functionalized Chitosan-Based Composite Nanoparticles	39:91	Functionalized Chitosan-Based Composite Nanoparticles as a Vaccine Adjuvant for Intranasal Delivery	39:137	Enhanced Immune Responses to Mucosa by Functionalized Chitosan-Based Composite Nanoparticles as a Vaccine Adjuvant for Intranasal Delivery.					
36382954	1	77	theme	injectable	342:351	arg1	administration					353:366	traditional injectable administration	330:366	traditional injectable administration	330:366	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	2	78	theme	delivery	407:414	arg1	systems					416:422	mucosal vaccine and adjuvant delivery systems	378:422	systems	416:422	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	4	79	theme	molecular	895:903	arg1	capacity					831:838	the potential capacity	817:838	the potential capacity of the N-2-HACC/CMCS NPs	817:863	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	79	theme	molecular	895:903	arg1	mechanism					905:913	the molecular mechanism	891:913	the molecular mechanism for the induction of enhanced mucosal and systemic immune responses	891:981	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	2	80	theme	mucosal	378:384	arg1	vaccine					386:392	mucosal vaccine and adjuvant delivery systems	378:422	vaccine	386:392	However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded.					
36382954	6	81	theme	viral	1602:1606	arg1	diseases					1619:1626	viral infectious diseases	1602:1626	viral infectious diseases	1602:1626	This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.					
36382954	5	82	theme	great	1230:1234	arg1	biostability					1236:1247	great biostability	1230:1247	great biostability	1230:1247	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	3	83	theme	enhanced	730:737	arg1	responses					746:754	enhanced immune responses	730:754	enhanced immune responses	730:754	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	5	84	theme	immune	1448:1453	arg1	responses					1455:1463	specific mucosal and systemic immune responses	1418:1463	specific mucosal and systemic immune responses	1418:1463	It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses.					
36382954	3	85	theme	chloride	584:591	arg1	chitosan/N					593:602	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	547:602	N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N	547:602	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	1	86	theme	local	254:258	arg1	responses					279:287	local or systemic immune responses	254:287	local or systemic immune responses	254:287	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
36382954	4	87	theme	N-2-HACC/CMCS/BSA	1145:1161	arg1	vaccine					1136:1142	a BSA-loaded N-2-HACC/CMCS adjuvant vaccine	1100:1142	a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs)	1100:1166	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	4	87	theme	N-2-HACC/CMCS/BSA	1145:1161	arg1	NPs					1163:1165	N-2-HACC/CMCS/BSA NPs	1145:1165	N-2-HACC/CMCS/BSA NPs	1145:1165	To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs).					
36382954	3	88	with	nanoparticles	629:641	arg1	absorption					686:695	excellent mucosal absorption	668:695	excellent mucosal absorption	668:695	In this work, N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method.					
36382954	1	89	theme	systemic	263:270	arg1	responses					279:287	local or systemic immune responses	254:287	local or systemic immune responses	254:287	Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration.					
35510317	4	0	theme	high	1009:1012	arg1	content					1020:1026	high water content	1009:1026	high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis	1009:1119	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	4	0	theme	high	1009:1012	arg1	%					1032:1032	≈94%	1029:1032	≈94%	1029:1032	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	5	1	theme	Digital	1194:1200	arg1	resins					1219:1224	low-viscosity Digital Light Processing resins	1180:1224	low-viscosity Digital Light Processing resins	1180:1224	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	5	1	theme	Digital	1194:1200	arg1	hydrogels					1151:1159	the IPN hydrogels	1143:1159	the IPN hydrogels	1143:1159	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	2	2	theme	polymer	385:391	arg1	hydrogels					407:415	interpenetrating polymer network (IPN) hydrogels	368:415	interpenetrating polymer network (IPN) hydrogels	368:415	These features are important for many applications, including as biomedical materials; however, the processing of interpenetrating polymer network (IPN) hydrogels is often limited by their multistep fabrication procedures.					
35510317	4	3	theme	excellent	1056:1064	arg1	work					1066:1069	excellent work	1056:1069	excellent work of fracture, tensile strength, and low hysteresis	1056:1119	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	5	4	theme	Processing	1208:1217	arg1	resins					1219:1224	low-viscosity Digital Light Processing resins	1180:1224	low-viscosity Digital Light Processing resins	1180:1224	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	5	4	theme	Processing	1208:1217	arg1	hydrogels					1151:1159	the IPN hydrogels	1143:1159	the IPN hydrogels	1143:1159	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	7	5	theme	IPN	1508:1510	arg1	hydrogels					1512:1520	these IPN hydrogels	1502:1520	these IPN hydrogels	1502:1520	Overall, the enhanced processing of these IPN hydrogels will expand their utility across applications.					
35510317	7	6	theme	hydrogels	1512:1520	arg1	processing					1488:1497	the enhanced processing	1475:1497	the enhanced processing of these IPN hydrogels	1475:1520	Overall, the enhanced processing of these IPN hydrogels will expand their utility across applications.					
35510317	3	7	theme	biopolymer	521:530	arg1	hydrogels					536:544	biopolymer IPN hydrogels	521:544	biopolymer IPN hydrogels	521:544	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	4	8	theme	fracture	1074:1081	arg1	work					1066:1069	excellent work	1056:1069	excellent work of fracture, tensile strength, and low hysteresis	1056:1119	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	3	9	with	networks	607:614	arg1	light					621:625	light	621:625	light	621:625	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	2	10	theme	interpenetrating	368:383	arg1	hydrogels					407:415	interpenetrating polymer network (IPN) hydrogels	368:415	interpenetrating polymer network (IPN) hydrogels	368:415	These features are important for many applications, including as biomedical materials; however, the processing of interpenetrating polymer network (IPN) hydrogels is often limited by their multistep fabrication procedures.					
35510317	3	11	with	HA	793:794	arg1	assemblies					822:831	thiolated guest-host assemblies	801:831	thiolated guest-host assemblies of adamantane and β-cyclodextrin	801:864	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	2	12	theme	as	316:317	arg1	materials					330:338	as biomedical materials	316:338	as biomedical materials	316:338	These features are important for many applications, including as biomedical materials; however, the processing of interpenetrating polymer network (IPN) hydrogels is often limited by their multistep fabrication procedures.					
35510317	2	13	theme	biomedical	319:328	arg1	materials					330:338	as biomedical materials	316:338	as biomedical materials	316:338	These features are important for many applications, including as biomedical materials; however, the processing of interpenetrating polymer network (IPN) hydrogels is often limited by their multistep fabrication procedures.					
35510317	1	14	theme	mechanical	189:198	arg1	properties					200:209	mechanical properties	189:209	mechanical properties	189:209	The incorporation of a secondary network into traditional single-network hydrogels can enhance mechanical properties, such as toughness and loading to failure.					
35510317	1	14	theme	mechanical	189:198	arg1	loading					234:240	loading	234:240	loading	234:240	The incorporation of a secondary network into traditional single-network hydrogels can enhance mechanical properties, such as toughness and loading to failure.					
35510317	1	14	theme	mechanical	189:198	arg1	toughness					220:228	toughness	220:228	toughness	220:228	The incorporation of a secondary network into traditional single-network hydrogels can enhance mechanical properties, such as toughness and loading to failure.					
35510317	3	15	theme	simultaneous	562:573	arg1	crosslinking					575:586	the simultaneous crosslinking	558:586	the simultaneous crosslinking of two independent networks with light, namely	558:633	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	5	16	theme	deformable	1334:1343	arg1	microparticles					1345:1358	deformable microparticles	1334:1358	deformable microparticles	1334:1358	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	3	17	theme	β-cyclodextrin	851:864	arg1	assemblies					822:831	thiolated guest-host assemblies	801:831	thiolated guest-host assemblies of adamantane and β-cyclodextrin	801:864	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	4	18	theme	network	983:989	arg1	composition					991:1001	the network composition	979:1001	the network composition	979:1001	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	7	19	theme	enhanced	1479:1486	arg1	processing					1488:1497	the enhanced processing	1475:1497	the enhanced processing of these IPN hydrogels	1475:1520	Overall, the enhanced processing of these IPN hydrogels will expand their utility across applications.					
35510317	6	20	theme	adhesive	1447:1454	arg1	peptides					1456:1463	adhesive peptides	1447:1463	adhesive peptides	1447:1463	Further, the IPNs are cytocompatible with cell adhesion dependent on the inclusion of adhesive peptides.					
35510317	3	21	theme	primary	723:729	arg1	network					731:737	the primary network	719:737	the primary network	719:737	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	6	22	theme	peptides	1456:1463	arg1	inclusion					1434:1442	the inclusion	1430:1442	the inclusion of adhesive peptides	1430:1463	Further, the IPNs are cytocompatible with cell adhesion dependent on the inclusion of adhesive peptides.					
35510317	2	23	theme	hydrogels	407:415	arg1	processing					354:363	the processing	350:363	the processing of interpenetrating polymer network (IPN) hydrogels	350:415	These features are important for many applications, including as biomedical materials; however, the processing of interpenetrating polymer network (IPN) hydrogels is often limited by their multistep fabrication procedures.					
35510317	0	24	theme	One-Pot	13:19	arg1	Formation					46:54	Simultaneous One-Pot Interpenetrating Network Formation	0:54	Simultaneous One-Pot Interpenetrating Network Formation	0:54	Simultaneous One-Pot Interpenetrating Network Formation to Expand 3D Processing Capabilities.					
35510317	3	25	theme	hydrogels	536:544	arg1	synthesis					508:516	the synthesis	504:516	the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely	504:633	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	0	26	theme	Simultaneous	0:11	arg1	Formation					46:54	Simultaneous One-Pot Interpenetrating Network Formation	0:54	Simultaneous One-Pot Interpenetrating Network Formation	0:54	Simultaneous One-Pot Interpenetrating Network Formation to Expand 3D Processing Capabilities.					
35510317	3	27	dep	form	869:872	arg1	ii					743:744	ii	743:744	ii	743:744	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	3	28	theme	independent	595:605	arg1	networks					607:614	two independent networks	591:614	two independent networks	591:614	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	4	29	theme	low	1106:1108	arg1	hysteresis					1110:1119	low hysteresis	1106:1119	low hysteresis	1106:1119	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	2	30	theme	IPN	402:404	arg1	hydrogels					407:415	interpenetrating polymer network (IPN) hydrogels	368:415	interpenetrating polymer network (IPN) hydrogels	368:415	These features are important for many applications, including as biomedical materials; however, the processing of interpenetrating polymer network (IPN) hydrogels is often limited by their multistep fabrication procedures.					
35510317	0	31	theme	Network	38:44	arg1	Formation					46:54	Simultaneous One-Pot Interpenetrating Network Formation	0:54	Simultaneous One-Pot Interpenetrating Network Formation	0:54	Simultaneous One-Pot Interpenetrating Network Formation to Expand 3D Processing Capabilities.					
35510317	5	32	theme	low-viscosity	1180:1192	arg1	resins					1219:1224	low-viscosity Digital Light Processing resins	1180:1224	low-viscosity Digital Light Processing resins	1180:1224	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	5	32	theme	low-viscosity	1180:1192	arg1	hydrogels					1151:1159	the IPN hydrogels	1143:1159	the IPN hydrogels	1143:1159	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	4	33	theme	hydrogels	947:955	arg1	properties					925:934	The mechanical properties	910:934	The mechanical properties of the IPN hydrogels	910:955	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	2	34	theme	multistep	443:451	arg1	procedures					465:474	their multistep fabrication procedures	437:474	their multistep fabrication procedures	437:474	These features are important for many applications, including as biomedical materials; however, the processing of interpenetrating polymer network (IPN) hydrogels is often limited by their multistep fabrication procedures.					
35510317	3	35	dep	form	714:717	arg1	i					636:636	i	636:636	i	636:636	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	4	36	theme	IPN	943:945	arg1	hydrogels					947:955	the IPN hydrogels	939:955	the IPN hydrogels	939:955	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	1	37	theme	secondary	117:125	arg1	network					127:133	a secondary network	115:133	a secondary network	115:133	The incorporation of a secondary network into traditional single-network hydrogels can enhance mechanical properties, such as toughness and loading to failure.					
35510317	3	38	theme	one-pot	485:491	arg1	scheme					493:498	a one-pot scheme	483:498	a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network	483:894	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	4	39	theme	content	1020:1026	arg1	hydrogels					1035:1043	high water content (≈94%) hydrogels	1009:1043	high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis	1009:1119	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	3	40	theme	thiol-ene	747:755	arg1	crosslinking					757:768	thiol-ene crosslinking	747:768	thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin	747:864	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	3	41	theme	methacrylate-modified	668:688	arg1	acid					701:704	methacrylate-modified hyaluronic acid	668:704	methacrylate-modified hyaluronic acid (HA)	668:709	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	3	41	theme	methacrylate-modified	668:688	arg1	HA					707:708	HA	707:708	HA	707:708	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	4	42	theme	strength	1092:1099	arg1	work					1066:1069	excellent work	1056:1069	excellent work of fracture, tensile strength, and low hysteresis	1056:1119	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	4	43	theme	water	1014:1018	arg1	content					1020:1026	high water content	1009:1026	high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis	1009:1119	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	4	43	theme	water	1014:1018	arg1	%					1032:1032	≈94%	1029:1032	≈94%	1029:1032	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	5	44	theme	IPN	1147:1149	arg1	proof-of-concept					1125:1140	proof-of-concept	1125:1140	proof-of-concept	1125:1140	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	5	44	theme	IPN	1147:1149	arg1	hydrogels					1151:1159	the IPN hydrogels	1143:1159	the IPN hydrogels	1143:1159	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	5	44	theme	IPN	1147:1149	arg1	resins					1219:1224	low-viscosity Digital Light Processing resins	1180:1224	low-viscosity Digital Light Processing resins	1180:1224	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	3	45	theme	free-radical	639:650	arg1	crosslinking					652:663	free-radical crosslinking	639:663	free-radical crosslinking of methacrylate-modified hyaluronic acid (HA)	639:709	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	2	46	theme	fabrication	453:463	arg1	procedures					465:474	their multistep fabrication procedures	437:474	their multistep fabrication procedures	437:474	These features are important for many applications, including as biomedical materials; however, the processing of interpenetrating polymer network (IPN) hydrogels is often limited by their multistep fabrication procedures.					
35510317	5	47	theme	Light	1202:1206	arg1	resins					1219:1224	low-viscosity Digital Light Processing resins	1180:1224	low-viscosity Digital Light Processing resins	1180:1224	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	5	47	theme	Light	1202:1206	arg1	hydrogels					1151:1159	the IPN hydrogels	1143:1159	the IPN hydrogels	1143:1159	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	0	48	theme	3D	66:67	arg1	Capabilities					80:91	3D Processing Capabilities	66:91	3D Processing Capabilities	66:91	Simultaneous One-Pot Interpenetrating Network Formation to Expand 3D Processing Capabilities.					
35510317	3	49	theme	thiolated	801:809	arg1	assemblies					822:831	thiolated guest-host assemblies	801:831	thiolated guest-host assemblies of adamantane and β-cyclodextrin	801:864	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	6	50	theme	cell	1403:1406	arg1	adhesion					1408:1415	cell adhesion	1403:1415	cell adhesion dependent on the inclusion of adhesive peptides	1403:1463	Further, the IPNs are cytocompatible with cell adhesion dependent on the inclusion of adhesive peptides.					
35510317	3	51	theme	networks	607:614	arg1	crosslinking					575:586	the simultaneous crosslinking	558:586	the simultaneous crosslinking of two independent networks with light, namely	558:633	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	6	52	with	cytocompatible	1383:1396	arg1	adhesion					1408:1415	cell adhesion	1403:1415	cell adhesion dependent on the inclusion of adhesive peptides	1403:1463	Further, the IPNs are cytocompatible with cell adhesion dependent on the inclusion of adhesive peptides.					
35510317	1	53	theme	network	127:133	arg1	incorporation					98:110	The incorporation	94:110	The incorporation of a secondary network into traditional single-network hydrogels	94:175	The incorporation of a secondary network into traditional single-network hydrogels can enhance mechanical properties, such as toughness and loading to failure.					
35510317	6	54	theme	dependent	1417:1425	arg1	adhesion					1408:1415	cell adhesion	1403:1415	cell adhesion dependent on the inclusion of adhesive peptides	1403:1463	Further, the IPNs are cytocompatible with cell adhesion dependent on the inclusion of adhesive peptides.					
35510317	3	55	theme	norbornene-modified	773:791	arg1	HA					793:794	norbornene-modified HA	773:794	norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin	773:864	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	4	56	theme	mechanical	914:923	arg1	properties					925:934	The mechanical properties	910:934	The mechanical properties of the IPN hydrogels	910:955	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	4	57	theme	tensile	1084:1090	arg1	strength					1092:1099	tensile strength	1084:1099	tensile strength	1084:1099	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	3	58	theme	HA	793:794	arg1	crosslinking					757:768	thiol-ene crosslinking	747:768	thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin	747:864	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	2	59	theme	many	287:290	arg1	materials					330:338	as biomedical materials	316:338	as biomedical materials	316:338	These features are important for many applications, including as biomedical materials; however, the processing of interpenetrating polymer network (IPN) hydrogels is often limited by their multistep fabrication procedures.					
35510317	2	59	theme	many	287:290	arg1	applications					292:303	many applications	287:303	many applications	287:303	These features are important for many applications, including as biomedical materials; however, the processing of interpenetrating polymer network (IPN) hydrogels is often limited by their multistep fabrication procedures.					
35510317	1	60	theme	traditional	140:150	arg1	hydrogels					167:175	traditional single-network hydrogels	140:175	traditional single-network hydrogels	140:175	The incorporation of a secondary network into traditional single-network hydrogels can enhance mechanical properties, such as toughness and loading to failure.					
35510317	5	61	theme	complex	1239:1245	arg1	structures					1247:1256	complex structures	1239:1256	complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles	1239:1358	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	3	62	theme	hyaluronic	690:699	arg1	acid					701:704	methacrylate-modified hyaluronic acid	668:704	methacrylate-modified hyaluronic acid (HA)	668:709	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	3	62	theme	hyaluronic	690:699	arg1	HA					707:708	HA	707:708	HA	707:708	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	3	63	dep	scheme	493:498	arg1	form					714:717	form	714:717	to form the primary network	711:737	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	3	63	dep	scheme	493:498	arg1	form					869:872	form	869:872	to form the secondary network	866:894	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	1	64	theme	single-network	152:165	arg1	hydrogels					167:175	traditional single-network hydrogels	140:175	traditional single-network hydrogels	140:175	The incorporation of a secondary network into traditional single-network hydrogels can enhance mechanical properties, such as toughness and loading to failure.					
35510317	3	65	theme	adamantane	836:845	arg1	assemblies					822:831	thiolated guest-host assemblies	801:831	thiolated guest-host assemblies of adamantane and β-cyclodextrin	801:864	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	4	66	theme	hysteresis	1110:1119	arg1	work					1066:1069	excellent work	1056:1069	excellent work of fracture, tensile strength, and low hysteresis	1056:1119	The mechanical properties of the IPN hydrogels are tuned by changing the network composition, with high water content (≈94%) hydrogels exhibiting excellent work of fracture, tensile strength, and low hysteresis.					
35510317	3	67	theme	acid	701:704	arg1	crosslinking					652:663	free-radical crosslinking	639:663	free-radical crosslinking of methacrylate-modified hyaluronic acid (HA)	639:709	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	3	68	theme	IPN	532:534	arg1	hydrogels					536:544	biopolymer IPN hydrogels	521:544	biopolymer IPN hydrogels	521:544	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	3	69	theme	secondary	878:886	arg1	network					888:894	the secondary network	874:894	the secondary network	874:894	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	3	70	theme	guest-host	811:820	arg1	assemblies					822:831	thiolated guest-host assemblies	801:831	thiolated guest-host assemblies of adamantane and β-cyclodextrin	801:864	Here, a one-pot scheme for the synthesis of biopolymer IPN hydrogels mediated by the simultaneous crosslinking of two independent networks with light, namely: i) free-radical crosslinking of methacrylate-modified hyaluronic acid (HA) to form the primary network and ii) thiol-ene crosslinking of norbornene-modified HA with thiolated guest-host assemblies of adamantane and β-cyclodextrin to form the secondary network, is reported.					
35510317	0	71	theme	Processing	69:78	arg1	Capabilities					80:91	3D Processing Capabilities	66:91	3D Processing Capabilities	66:91	Simultaneous One-Pot Interpenetrating Network Formation to Expand 3D Processing Capabilities.					
35510317	5	72	theme	microfluidic	1305:1316	arg1	devices					1318:1324	microfluidic devices	1305:1324	microfluidic devices	1305:1324	As proof-of-concept, the IPN hydrogels are implemented as low-viscosity Digital Light Processing resins to fabricate complex structures that recover shape upon loading, as well as in microfluidic devices to form deformable microparticles.					
35510317	0	73	theme	Interpenetrating	21:36	arg1	Formation					46:54	Simultaneous One-Pot Interpenetrating Network Formation	0:54	Simultaneous One-Pot Interpenetrating Network Formation	0:54	Simultaneous One-Pot Interpenetrating Network Formation to Expand 3D Processing Capabilities.					
35510317	2	74	theme	network	393:399	arg1	hydrogels					407:415	interpenetrating polymer network (IPN) hydrogels	368:415	interpenetrating polymer network (IPN) hydrogels	368:415	These features are important for many applications, including as biomedical materials; however, the processing of interpenetrating polymer network (IPN) hydrogels is often limited by their multistep fabrication procedures.					
36933599	5	0	theme	actuation	1064:1072	arg1	performance					1074:1084	excellent actuation performance	1054:1084	excellent actuation performance	1054:1084	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	2	1	theme	raw	367:369	arg1	materials					371:379	cellulose raw materials	357:379	cellulose raw materials	357:379	In this paper, highly visible transparent cellulose-based UV-absorbing films were prepared by dissolving cellulose raw materials using N-methylmorpholine-N-oxide (NMMO) and using ZnO nanoparticles as UV absorbers.					
36933599	0	2	theme	high	72:75	arg1	sensitivity					77:87	high sensitivity	72:87	high sensitivity	72:87	UV and IR dual light triggered cellulose-based invisible actuators with high sensitivity.					
36933599	5	3	theme	maximum	1119:1125	arg1	curvature					1135:1143	a maximum bending curvature	1117:1143	a maximum bending curvature of 3.0 cm-1	1117:1155	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	4	4	theme	ZnO	846:848	arg1	NPs					850:852	ZnO NPs	846:852	ZnO NPs	846:852	In addition to its sensitive response to Infrared (IR) light, the as-prepared actuator also shows a highly sensitive response to UV light, which is attributed to the strong absorption of UV light by ZnO NPs.					
36933599	2	5	theme	cellulose	357:365	arg1	materials					371:379	cellulose raw materials	357:379	cellulose raw materials	357:379	In this paper, highly visible transparent cellulose-based UV-absorbing films were prepared by dissolving cellulose raw materials using N-methylmorpholine-N-oxide (NMMO) and using ZnO nanoparticles as UV absorbers.					
36933599	5	6	theme	bending	1127:1133	arg1	curvature					1135:1143	a maximum bending curvature	1117:1143	a maximum bending curvature of 3.0 cm-1	1117:1155	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	7	from	differences	877:887	arg1	capacity					903:910	adsorption capacity	892:910	adsorption capacity	892:910	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	2	8	theme	visible	274:280	arg1	films					323:327	highly visible transparent cellulose-based UV-absorbing films	267:327	highly visible transparent cellulose-based UV-absorbing films	267:327	In this paper, highly visible transparent cellulose-based UV-absorbing films were prepared by dissolving cellulose raw materials using N-methylmorpholine-N-oxide (NMMO) and using ZnO nanoparticles as UV absorbers.					
36933599	1	9	used	used	111:114	arg2	Actuators					90:98	Actuators	90:98	Actuators	90:98	Actuators are widely used in bionic devices and soft robots, among which invisible actuators have some unique applications, including performing secret missions.					
36933599	5	10	theme	8 s.	1187:1190	arg1	bug					1199:1201	8 s. Bionic bug	1187:1201	8 s. Bionic bug	1187:1201	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	11	theme	force	1094:1098	arg1	density					1100:1106	a force density	1092:1106	a force density of 60.5	1092:1114	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	12	theme	drastic	869:875	arg1	differences					877:887	the drastic differences	865:887	the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules	865:968	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	13	theme	Bionic	1192:1197	arg1	bug					1199:1201	8 s. Bionic bug	1187:1201	8 s. Bionic bug	1187:1201	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	4	14	theme	UV	834:835	arg1	light					837:841	UV light	834:841	UV light	834:841	In addition to its sensitive response to Infrared (IR) light, the as-prepared actuator also shows a highly sensitive response to UV light, which is attributed to the strong absorption of UV light by ZnO NPs.					
36933599	0	15	with	actuators	57:65	arg1	sensitivity					77:87	high sensitivity	72:87	high sensitivity	72:87	UV and IR dual light triggered cellulose-based invisible actuators with high sensitivity.					
36933599	3	16	theme	polytetrafluoroethylene	561:583	arg1	film					592:595	highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film	526:595	highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film	526:595	Furthermore, transparent actuator was fabricated by growing highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film on regenerated cellulose (RC)-ZnO composite film.					
36933599	4	17	theme	UV	776:777	arg1	light					779:783	UV light	776:783	UV light	776:783	In addition to its sensitive response to Infrared (IR) light, the as-prepared actuator also shows a highly sensitive response to UV light, which is attributed to the strong absorption of UV light by ZnO NPs.					
36933599	5	18	theme	60.5	1111:1114	arg1	curvature					1135:1143	a maximum bending curvature	1117:1143	a maximum bending curvature of 3.0 cm-1	1117:1155	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	18	theme	60.5	1111:1114	arg1	density					1100:1106	a force density	1092:1106	a force density of 60.5	1092:1114	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	18	theme	60.5	1111:1114	arg1	time					1173:1176	a response time	1162:1176	a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights	1162:1313	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	19	theme	smart	1204:1208	arg1	door					1210:1213	smart door	1204:1213	smart door	1204:1213	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	3	20	theme	regenerated	600:610	arg1	film					641:644	regenerated cellulose (RC)-ZnO composite film	600:644	regenerated cellulose (RC)-ZnO composite film	600:644	Furthermore, transparent actuator was fabricated by growing highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film on regenerated cellulose (RC)-ZnO composite film.					
36933599	1	21	theme	invisible	163:171	arg1	actuators					173:181	invisible actuators	163:181	invisible actuators	163:181	Actuators are widely used in bionic devices and soft robots, among which invisible actuators have some unique applications, including performing secret missions.					
36933599	4	22	theme	light	837:841	arg1	absorption					820:829	the strong absorption	809:829	the strong absorption of UV light by ZnO NPs	809:852	In addition to its sensitive response to Infrared (IR) light, the as-prepared actuator also shows a highly sensitive response to UV light, which is attributed to the strong absorption of UV light by ZnO NPs.					
36933599	2	23	theme	UV-absorbing	310:321	arg1	films					323:327	highly visible transparent cellulose-based UV-absorbing films	267:327	highly visible transparent cellulose-based UV-absorbing films	267:327	In this paper, highly visible transparent cellulose-based UV-absorbing films were prepared by dissolving cellulose raw materials using N-methylmorpholine-N-oxide (NMMO) and using ZnO nanoparticles as UV absorbers.					
36933599	5	24	theme	actuator	1254:1261	arg1	all					1263:1265	the actuator all	1250:1265	the actuator all	1250:1265	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	4	25	theme	Infrared	688:695	arg1	light					702:706	Infrared (IR) light	688:706	Infrared (IR) light	688:706	In addition to its sensitive response to Infrared (IR) light, the as-prepared actuator also shows a highly sensitive response to UV light, which is attributed to the strong absorption of UV light by ZnO NPs.					
36933599	2	26	theme	UV	452:453	arg1	absorbers					455:463	UV absorbers	452:463	UV absorbers	452:463	In this paper, highly visible transparent cellulose-based UV-absorbing films were prepared by dissolving cellulose raw materials using N-methylmorpholine-N-oxide (NMMO) and using ZnO nanoparticles as UV absorbers.					
36933599	5	27	theme	asymmetrically-	975:989	arg1	actuator					1001:1008	the asymmetrically- assembled actuator	971:1008	the asymmetrically- assembled actuator	971:1008	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	28	theme	high	1033:1036	arg1	sensitivity					1038:1048	extremely high sensitivity	1023:1048	extremely high sensitivity	1023:1048	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	2	29	theme	cellulose-based	294:308	arg1	films					323:327	highly visible transparent cellulose-based UV-absorbing films	267:327	highly visible transparent cellulose-based UV-absorbing films	267:327	In this paper, highly visible transparent cellulose-based UV-absorbing films were prepared by dissolving cellulose raw materials using N-methylmorpholine-N-oxide (NMMO) and using ZnO nanoparticles as UV absorbers.					
36933599	3	30	theme	transparent	479:489	arg1	actuator					491:498	transparent actuator	479:498	transparent actuator	479:498	Furthermore, transparent actuator was fabricated by growing highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film on regenerated cellulose (RC)-ZnO composite film.					
36933599	5	31	theme	RC-ZnO	924:929	arg1	materials					940:948	the RC-ZnO and PTFE materials	920:948	materials	940:948	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	32	theme	response	1164:1171	arg1	time					1173:1176	a response time	1162:1176	a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights	1162:1313	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	33	theme	water	954:958	arg1	molecules					960:968	water molecules	954:968	water molecules	954:968	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	2	34	theme	transparent	282:292	arg1	films					323:327	highly visible transparent cellulose-based UV-absorbing films	267:327	highly visible transparent cellulose-based UV-absorbing films	267:327	In this paper, highly visible transparent cellulose-based UV-absorbing films were prepared by dissolving cellulose raw materials using N-methylmorpholine-N-oxide (NMMO) and using ZnO nanoparticles as UV absorbers.					
36933599	2	35	theme	ZnO	431:433	arg1	nanoparticles					435:447	ZnO nanoparticles	431:447	ZnO nanoparticles as UV absorbers	431:463	In this paper, highly visible transparent cellulose-based UV-absorbing films were prepared by dissolving cellulose raw materials using N-methylmorpholine-N-oxide (NMMO) and using ZnO nanoparticles as UV absorbers.					
36933599	5	36	theme	assembled	991:999	arg1	actuator					1001:1008	the asymmetrically- assembled actuator	971:1008	the asymmetrically- assembled actuator	971:1008	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	4	37	theme	sensitive	666:674	arg1	response					676:683	its sensitive response	662:683	its sensitive response to Infrared (IR) light	662:706	In addition to its sensitive response to Infrared (IR) light, the as-prepared actuator also shows a highly sensitive response to UV light, which is attributed to the strong absorption of UV light by ZnO NPs.					
36933599	5	38	theme	sensitive	1275:1283	arg1	responses					1285:1293	sensitive responses	1275:1293	sensitive responses to UV and IR lights	1275:1313	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	1	39	theme	unique	193:198	arg1	missions					242:249	performing secret missions	224:249	performing secret missions	224:249	Actuators are widely used in bionic devices and soft robots, among which invisible actuators have some unique applications, including performing secret missions.					
36933599	1	39	theme	unique	193:198	arg1	applications					200:211	some unique applications	188:211	some unique applications	188:211	Actuators are widely used in bionic devices and soft robots, among which invisible actuators have some unique applications, including performing secret missions.					
36933599	0	40	theme	dual	10:13	arg1	light					15:19	dual light	10:19	dual light	10:19	UV and IR dual light triggered cellulose-based invisible actuators with high sensitivity.					
36933599	4	41	theme	sensitive	754:762	arg1	response					764:771	a highly sensitive response	745:771	a highly sensitive response to UV light, which is attributed to the strong absorption of UV light by ZnO NPs	745:852	In addition to its sensitive response to Infrared (IR) light, the as-prepared actuator also shows a highly sensitive response to UV light, which is attributed to the strong absorption of UV light by ZnO NPs.					
36933599	0	42	theme	invisible	47:55	arg1	actuators					57:65	cellulose-based invisible actuators	31:65	cellulose-based invisible actuators with high sensitivity	31:87	UV and IR dual light triggered cellulose-based invisible actuators with high sensitivity.					
36933599	4	43	theme	as-prepared	713:723	arg1	actuator					725:732	the as-prepared actuator	709:732	the as-prepared actuator	709:732	In addition to its sensitive response to Infrared (IR) light, the as-prepared actuator also shows a highly sensitive response to UV light, which is attributed to the strong absorption of UV light by ZnO NPs.					
36933599	0	44	theme	cellulose-based	31:45	arg1	actuators					57:65	cellulose-based invisible actuators	31:65	cellulose-based invisible actuators with high sensitivity	31:87	UV and IR dual light triggered cellulose-based invisible actuators with high sensitivity.					
36933599	5	45	theme	3.0 cm-1	1148:1155	arg1	curvature					1135:1143	a maximum bending curvature	1117:1143	a maximum bending curvature of 3.0 cm-1	1117:1155	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	45	theme	3.0 cm-1	1148:1155	arg1	density					1100:1106	a force density	1092:1106	a force density of 60.5	1092:1114	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	45	theme	3.0 cm-1	1148:1155	arg1	time					1173:1176	a response time	1162:1176	a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights	1162:1313	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	4	46	theme	strong	813:818	arg1	absorption					820:829	the strong absorption	809:829	the strong absorption of UV light by ZnO NPs	809:852	In addition to its sensitive response to Infrared (IR) light, the as-prepared actuator also shows a highly sensitive response to UV light, which is attributed to the strong absorption of UV light by ZnO NPs.					
36933599	3	47	theme	transparent	533:543	arg1	film					592:595	highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film	526:595	highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film	526:595	Furthermore, transparent actuator was fabricated by growing highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film on regenerated cellulose (RC)-ZnO composite film.					
36933599	5	48	theme	adsorption	892:901	arg1	capacity					903:910	adsorption capacity	892:910	adsorption capacity	892:910	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	49	theme	excavator	1230:1238	arg1	arm					1223:1225	the arm	1219:1225	the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights	1219:1313	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	49	theme	excavator	1230:1238	arg1	excavator					1230:1238	excavator	1230:1238	excavator made from the actuator all exhibit sensitive responses to UV and IR lights	1230:1313	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	49	theme	excavator	1230:1238	arg1	bug					1199:1201	8 s. Bionic bug	1187:1201	8 s. Bionic bug	1187:1201	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	5	49	theme	excavator	1230:1238	arg1	door					1210:1213	smart door	1204:1213	smart door	1204:1213	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	4	50	dep	response	676:683	arg1	addition					650:657	addition	650:657	addition	650:657	In addition to its sensitive response to Infrared (IR) light, the as-prepared actuator also shows a highly sensitive response to UV light, which is attributed to the strong absorption of UV light by ZnO NPs.					
36933599	3	51	theme	-ZnO	626:629	arg1	film					641:644	regenerated cellulose (RC)-ZnO composite film	600:644	regenerated cellulose (RC)-ZnO composite film	600:644	Furthermore, transparent actuator was fabricated by growing highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film on regenerated cellulose (RC)-ZnO composite film.					
36933599	1	52	theme	bionic	119:124	arg1	devices					126:132	bionic devices	119:132	bionic devices	119:132	Actuators are widely used in bionic devices and soft robots, among which invisible actuators have some unique applications, including performing secret missions.					
36933599	2	53	dep	dissolving	346:355	arg1	using					425:429	using	425:429	using ZnO nanoparticles as UV absorbers	425:463	In this paper, highly visible transparent cellulose-based UV-absorbing films were prepared by dissolving cellulose raw materials using N-methylmorpholine-N-oxide (NMMO) and using ZnO nanoparticles as UV absorbers.					
36933599	2	53	dep	dissolving	346:355	arg1	using					381:385	using	381:385	using N-methylmorpholine-N-oxide (NMMO)	381:419	In this paper, highly visible transparent cellulose-based UV-absorbing films were prepared by dissolving cellulose raw materials using N-methylmorpholine-N-oxide (NMMO) and using ZnO nanoparticles as UV absorbers.					
36933599	5	54	theme	excellent	1054:1062	arg1	performance					1074:1084	excellent actuation performance	1054:1084	excellent actuation performance	1054:1084	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	4	55	dep	Infrared	688:695	arg1	IR					698:699	IR	698:699	IR	698:699	In addition to its sensitive response to Infrared (IR) light, the as-prepared actuator also shows a highly sensitive response to UV light, which is attributed to the strong absorption of UV light by ZnO NPs.					
36933599	5	56	theme	IR	1305:1306	arg1	lights					1308:1313	IR lights	1305:1313	IR lights	1305:1313	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	3	57	theme	hydrophobic	549:559	arg1	film					592:595	highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film	526:595	highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film	526:595	Furthermore, transparent actuator was fabricated by growing highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film on regenerated cellulose (RC)-ZnO composite film.					
36933599	1	58	contain	have	183:186	arg2	applications					200:211	some unique applications	188:211	some unique applications	188:211	Actuators are widely used in bionic devices and soft robots, among which invisible actuators have some unique applications, including performing secret missions.					
36933599	1	58	contain	have	183:186	arg1	actuators					173:181	invisible actuators	163:181	invisible actuators	163:181	Actuators are widely used in bionic devices and soft robots, among which invisible actuators have some unique applications, including performing secret missions.					
36933599	1	58	contain	have	183:186	arg2	missions					242:249	performing secret missions	224:249	performing secret missions	224:249	Actuators are widely used in bionic devices and soft robots, among which invisible actuators have some unique applications, including performing secret missions.					
36933599	3	59	theme	composite	631:639	arg1	film					641:644	regenerated cellulose (RC)-ZnO composite film	600:644	regenerated cellulose (RC)-ZnO composite film	600:644	Furthermore, transparent actuator was fabricated by growing highly transparent and hydrophobic polytetrafluoroethylene (PTFE) film on regenerated cellulose (RC)-ZnO composite film.					
36933599	5	60	theme	PTFE	935:938	arg1	materials					940:948	the RC-ZnO and PTFE materials	920:948	materials	940:948	Thanks to the drastic differences in adsorption capacity between the RC-ZnO and PTFE materials for water molecules, the asymmetrically- assembled actuator demonstrates extremely high sensitivity and excellent actuation performance, with a force density of 60.5, a maximum bending curvature of 3.0 cm-1, and a response time of below 8 s. Bionic bug, smart door and the arm of excavator made from the actuator all exhibit sensitive responses to UV and IR lights.					
36933599	1	61	theme	performing	224:233	arg1	missions					242:249	performing secret missions	224:249	performing secret missions	224:249	Actuators are widely used in bionic devices and soft robots, among which invisible actuators have some unique applications, including performing secret missions.					
36933599	0	62	dep	UV	0:1	arg1	light					15:19	dual light	10:19	dual light	10:19	UV and IR dual light triggered cellulose-based invisible actuators with high sensitivity.					
36933599	1	63	theme	soft	138:141	arg1	robots					143:148	soft robots	138:148	soft robots	138:148	Actuators are widely used in bionic devices and soft robots, among which invisible actuators have some unique applications, including performing secret missions.					
36933599	1	64	theme	secret	235:240	arg1	missions					242:249	performing secret missions	224:249	performing secret missions	224:249	Actuators are widely used in bionic devices and soft robots, among which invisible actuators have some unique applications, including performing secret missions.					
36184130	4	0	dep	red	602:604	arg1	R					607:607	R	607:607	R	607:607	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	7	1	theme	new	1142:1144	arg1	discoveries					1146:1156	new discoveries	1142:1156	new discoveries in many areas of biomass refining and plant science	1142:1208	This low-cost, cell-scale method is expected to contribute to new discoveries in many areas of biomass refining and plant science.					
36184130	6	2	theme	cellulose	994:1002	arg1	profiles					971:978	The high-definition spatial in-situ simultaneous profiles	922:978	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations	922:1061	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations were visualized.					
36184130	1	3	theme	morphology	271:280	arg1	understandings					242:255	deeper understandings	235:255	deeper understandings of crop stalk morphology	235:280	There is a strong need for low-cost lignocellulosic composition simultaneous localization methodologies to benefit deeper understandings of crop stalk morphology.					
36184130	6	4	theme	simultaneous	958:969	arg1	profiles					971:978	The high-definition spatial in-situ simultaneous profiles	922:978	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations	922:1061	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations were visualized.					
36184130	7	5	dep	refining	1183:1190	arg1	science					1202:1208	science	1202:1208	science	1202:1208	This low-cost, cell-scale method is expected to contribute to new discoveries in many areas of biomass refining and plant science.					
36184130	1	6	theme	low-cost	147:154	arg1	composition					172:182	low-cost lignocellulosic composition simultaneous localization methodologies	147:222	low-cost lignocellulosic composition simultaneous localization methodologies	147:222	There is a strong need for low-cost lignocellulosic composition simultaneous localization methodologies to benefit deeper understandings of crop stalk morphology.					
36184130	6	7	theme	alkali-treated	1007:1020	arg1	tissues					1034:1040	alkali-treated maize stalk tissues	1007:1040	alkali-treated maize stalk tissues	1007:1040	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations were visualized.					
36184130	2	8	theme	quantitative	313:324	arg1	microscopy					371:380	a robust quantitative safranin O-fast green staining-based optical microscopy	304:380	a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues	304:496	This study developed a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues.					
36184130	5	9	dep	microscopy	878:887	arg1	methods					913:919	methods	913:919	methods	913:919	The method was validated on model rice with known bioinformatics, and the results were consistent with those of fluorescence microscopy and immunogold labeling methods.					
36184130	4	10	theme	cellulose	728:736	arg1	indicators					703:712	quantitative indicators	690:712	quantitative indicators of lignin and cellulose, respectively	690:750	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	4	10	theme	cellulose	728:736	arg1	ratios					581:586	The ratios	577:586	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity	577:658	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	4	11	dep	green	611:615	arg1	G					618:618	G	618:618	G	618:618	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	5	12	with	rice	787:790	arg1	bioinformatics					803:816	known bioinformatics	797:816	known bioinformatics	797:816	The method was validated on model rice with known bioinformatics, and the results were consistent with those of fluorescence microscopy and immunogold labeling methods.					
36184130	2	13	theme	robust	306:311	arg1	microscopy					371:380	a robust quantitative safranin O-fast green staining-based optical microscopy	304:380	a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues	304:496	This study developed a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues.					
36184130	3	14	theme	stained	548:554	arg1	images					556:561	stained images	548:561	stained images	548:561	Foreground extraction and dye residue removal of stained images were adapted.					
36184130	1	15	theme	lignocellulosic	156:170	arg1	composition					172:182	low-cost lignocellulosic composition simultaneous localization methodologies	147:222	low-cost lignocellulosic composition simultaneous localization methodologies	147:222	There is a strong need for low-cost lignocellulosic composition simultaneous localization methodologies to benefit deeper understandings of crop stalk morphology.					
36184130	0	16	theme	stained	99:105	arg1	micrographs					107:117	stained micrographs	99:117	stained micrographs	99:117	A novel in-situ quantitative profiling approach for visualizing changes in lignin and cellulose by stained micrographs.					
36184130	5	17	theme	immunogold	893:902	arg1	labeling					904:911	immunogold labeling	893:911	immunogold labeling	893:911	The method was validated on model rice with known bioinformatics, and the results were consistent with those of fluorescence microscopy and immunogold labeling methods.					
36184130	6	18	theme	maize	1022:1026	arg1	tissues					1034:1040	alkali-treated maize stalk tissues	1007:1040	alkali-treated maize stalk tissues	1007:1040	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations were visualized.					
36184130	7	19	theme	plant	1196:1200	arg1	areas					1166:1170	many areas	1161:1170	many areas of biomass refining and plant science	1161:1208	This low-cost, cell-scale method is expected to contribute to new discoveries in many areas of biomass refining and plant science.					
36184130	0	20	theme	novel	2:6	arg1	approach					39:46	A novel in-situ quantitative profiling approach	0:46	A novel in-situ quantitative profiling approach for visualizing changes in lignin and cellulose by stained micrographs	0:117	A novel in-situ quantitative profiling approach for visualizing changes in lignin and cellulose by stained micrographs.					
36184130	2	21	theme	staining-based	348:361	arg1	microscopy					371:380	a robust quantitative safranin O-fast green staining-based optical microscopy	304:380	a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues	304:496	This study developed a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues.					
36184130	1	22	theme	simultaneous	184:195	arg1	methodologies					210:222	simultaneous localization methodologies	184:222	low-cost lignocellulosic composition simultaneous localization methodologies	147:222	There is a strong need for low-cost lignocellulosic composition simultaneous localization methodologies to benefit deeper understandings of crop stalk morphology.					
36184130	5	23	theme	known	797:801	arg1	bioinformatics					803:816	known bioinformatics	797:816	known bioinformatics	797:816	The method was validated on model rice with known bioinformatics, and the results were consistent with those of fluorescence microscopy and immunogold labeling methods.					
36184130	6	24	theme	high-definition	926:940	arg1	profiles					971:978	The high-definition spatial in-situ simultaneous profiles	922:978	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations	922:1061	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations were visualized.					
36184130	7	25	theme	low-cost	1085:1092	arg1	method					1106:1111	This low-cost, cell-scale method	1080:1111	This low-cost, cell-scale method	1080:1111	This low-cost, cell-scale method is expected to contribute to new discoveries in many areas of biomass refining and plant science.					
36184130	2	26	theme	green	342:346	arg1	microscopy					371:380	a robust quantitative safranin O-fast green staining-based optical microscopy	304:380	a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues	304:496	This study developed a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues.					
36184130	1	27	theme	localization	197:208	arg1	methodologies					210:222	simultaneous localization methodologies	184:222	low-cost lignocellulosic composition simultaneous localization methodologies	147:222	There is a strong need for low-cost lignocellulosic composition simultaneous localization methodologies to benefit deeper understandings of crop stalk morphology.					
36184130	0	28	theme	quantitative	16:27	arg1	approach					39:46	A novel in-situ quantitative profiling approach	0:46	A novel in-situ quantitative profiling approach for visualizing changes in lignin and cellulose by stained micrographs	0:117	A novel in-situ quantitative profiling approach for visualizing changes in lignin and cellulose by stained micrographs.					
36184130	6	29	theme	lignin	983:988	arg1	profiles					971:978	The high-definition spatial in-situ simultaneous profiles	922:978	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations	922:1061	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations were visualized.					
36184130	2	30	theme	O-fast	335:340	arg1	microscopy					371:380	a robust quantitative safranin O-fast green staining-based optical microscopy	304:380	a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues	304:496	This study developed a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues.					
36184130	2	31	theme	stalk	484:488	arg1	tissues					490:496	stalk tissues	484:496	stalk tissues	484:496	This study developed a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues.					
36184130	0	32	theme	in-situ	8:14	arg1	approach					39:46	A novel in-situ quantitative profiling approach	0:46	A novel in-situ quantitative profiling approach for visualizing changes in lignin and cellulose by stained micrographs	0:117	A novel in-situ quantitative profiling approach for visualizing changes in lignin and cellulose by stained micrographs.					
36184130	4	33	theme	green	611:615	arg1	intensity					650:658	normalized red (R), green (G), and blue (B) channel signal intensity	591:658	normalized red (R), green (G), and blue (B) channel signal intensity	591:658	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	6	34	from	profiles	971:978	arg1	tissues					1034:1040	alkali-treated maize stalk tissues	1007:1040	alkali-treated maize stalk tissues	1007:1040	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations were visualized.					
36184130	6	34	from	profiles	971:978	arg1	variations					1052:1061	their variations	1046:1061	their variations	1046:1061	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations were visualized.					
36184130	2	35	theme	safranin	326:333	arg1	microscopy					371:380	a robust quantitative safranin O-fast green staining-based optical microscopy	304:380	a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues	304:496	This study developed a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues.					
36184130	5	36	theme	fluorescence	865:876	arg1	microscopy					878:887	fluorescence microscopy	865:887	fluorescence microscopy	865:887	The method was validated on model rice with known bioinformatics, and the results were consistent with those of fluorescence microscopy and immunogold labeling methods.					
36184130	4	37	theme	intensity	650:658	arg1	indicators					703:712	quantitative indicators	690:712	quantitative indicators of lignin and cellulose, respectively	690:750	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	4	37	theme	intensity	650:658	arg1	G/B					669:671	G/B	669:671	G/B	669:671	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	4	37	theme	intensity	650:658	arg1	R/B					661:663	R/B	661:663	R/B	661:663	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	4	37	theme	intensity	650:658	arg1	ratios					581:586	The ratios	577:586	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity	577:658	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	4	38	theme	signal	643:648	arg1	intensity					650:658	normalized red (R), green (G), and blue (B) channel signal intensity	591:658	normalized red (R), green (G), and blue (B) channel signal intensity	591:658	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	2	39	theme	digital	440:446	arg1	profiles					448:455	digital profiles	440:455	digital profiles of lignin and cellulose	440:479	This study developed a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues.					
36184130	2	40	theme	cellulose	471:479	arg1	profiles					448:455	digital profiles	440:455	digital profiles of lignin and cellulose	440:479	This study developed a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues.					
36184130	2	41	theme	lignin	460:465	arg1	profiles					448:455	digital profiles	440:455	digital profiles of lignin and cellulose	440:479	This study developed a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues.					
36184130	4	42	theme	normalized	591:600	arg1	intensity					650:658	normalized red (R), green (G), and blue (B) channel signal intensity	591:658	normalized red (R), green (G), and blue (B) channel signal intensity	591:658	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	0	43	theme	profiling	29:37	arg1	approach					39:46	A novel in-situ quantitative profiling approach	0:46	A novel in-situ quantitative profiling approach for visualizing changes in lignin and cellulose by stained micrographs	0:117	A novel in-situ quantitative profiling approach for visualizing changes in lignin and cellulose by stained micrographs.					
36184130	6	44	theme	stalk	1028:1032	arg1	tissues					1034:1040	alkali-treated maize stalk tissues	1007:1040	alkali-treated maize stalk tissues	1007:1040	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations were visualized.					
36184130	1	45	theme	deeper	235:240	arg1	understandings					242:255	deeper understandings	235:255	deeper understandings of crop stalk morphology	235:280	There is a strong need for low-cost lignocellulosic composition simultaneous localization methodologies to benefit deeper understandings of crop stalk morphology.					
36184130	5	46	theme	model	781:785	arg1	rice					787:790	model rice	781:790	model rice with known bioinformatics	781:816	The method was validated on model rice with known bioinformatics, and the results were consistent with those of fluorescence microscopy and immunogold labeling methods.					
36184130	6	47	theme	in-situ	950:956	arg1	profiles					971:978	The high-definition spatial in-situ simultaneous profiles	922:978	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations	922:1061	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations were visualized.					
36184130	0	48	from	changes	64:70	arg1	lignin					75:80	lignin	75:80	lignin	75:80	A novel in-situ quantitative profiling approach for visualizing changes in lignin and cellulose by stained micrographs.					
36184130	0	48	from	changes	64:70	arg1	cellulose					86:94	cellulose	86:94	cellulose	86:94	A novel in-situ quantitative profiling approach for visualizing changes in lignin and cellulose by stained micrographs.					
36184130	7	49	from	discoveries	1146:1156	arg1	areas					1166:1170	many areas	1161:1170	many areas of biomass refining and plant science	1161:1208	This low-cost, cell-scale method is expected to contribute to new discoveries in many areas of biomass refining and plant science.					
36184130	3	50	theme	images	556:561	arg1	extraction					510:519	Foreground extraction	499:519	Foreground extraction	499:519	Foreground extraction and dye residue removal of stained images were adapted.					
36184130	3	50	theme	images	556:561	arg1	removal					537:543	dye residue removal	525:543	dye residue removal	525:543	Foreground extraction and dye residue removal of stained images were adapted.					
36184130	7	51	theme	many	1161:1164	arg1	areas					1166:1170	many areas	1161:1170	many areas of biomass refining and plant science	1161:1208	This low-cost, cell-scale method is expected to contribute to new discoveries in many areas of biomass refining and plant science.					
36184130	6	52	theme	spatial	942:948	arg1	profiles					971:978	The high-definition spatial in-situ simultaneous profiles	922:978	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations	922:1061	The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations were visualized.					
36184130	1	53	dep	composition	172:182	arg1	methodologies					210:222	simultaneous localization methodologies	184:222	low-cost lignocellulosic composition simultaneous localization methodologies	147:222	There is a strong need for low-cost lignocellulosic composition simultaneous localization methodologies to benefit deeper understandings of crop stalk morphology.					
36184130	4	54	theme	quantitative	690:701	arg1	indicators					703:712	quantitative indicators	690:712	quantitative indicators of lignin and cellulose, respectively	690:750	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	4	54	theme	quantitative	690:701	arg1	ratios					581:586	The ratios	577:586	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity	577:658	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	3	55	theme	Foreground	499:508	arg1	extraction					510:519	Foreground extraction	499:519	Foreground extraction	499:519	Foreground extraction and dye residue removal of stained images were adapted.					
36184130	4	56	theme	red	602:604	arg1	intensity					650:658	normalized red (R), green (G), and blue (B) channel signal intensity	591:658	normalized red (R), green (G), and blue (B) channel signal intensity	591:658	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	5	57	with	consistent	840:849	arg1	those					856:860	those	856:860	those	856:860	The method was validated on model rice with known bioinformatics, and the results were consistent with those of fluorescence microscopy and immunogold labeling methods.					
36184130	4	58	theme	lignin	717:722	arg1	indicators					703:712	quantitative indicators	690:712	quantitative indicators of lignin and cellulose, respectively	690:750	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	4	58	theme	lignin	717:722	arg1	ratios					581:586	The ratios	577:586	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity	577:658	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	4	59	theme	blue	626:629	arg1	intensity					650:658	normalized red (R), green (G), and blue (B) channel signal intensity	591:658	normalized red (R), green (G), and blue (B) channel signal intensity	591:658	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	4	60	dep	blue	626:629	arg1	B					632:632	B	632:632	B	632:632	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	2	61	theme	optical	363:369	arg1	microscopy					371:380	a robust quantitative safranin O-fast green staining-based optical microscopy	304:380	a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues	304:496	This study developed a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues.					
36184130	3	62	theme	dye	525:527	arg1	removal					537:543	dye residue removal	525:543	dye residue removal	525:543	Foreground extraction and dye residue removal of stained images were adapted.					
36184130	3	63	theme	residue	529:535	arg1	removal					537:543	dye residue removal	525:543	dye residue removal	525:543	Foreground extraction and dye residue removal of stained images were adapted.					
36184130	1	64	theme	strong	131:136	arg1	need					138:141	a strong need	129:141	a strong need for low-cost lignocellulosic composition simultaneous localization methodologies to benefit deeper understandings of crop stalk morphology	129:280	There is a strong need for low-cost lignocellulosic composition simultaneous localization methodologies to benefit deeper understandings of crop stalk morphology.					
36184130	1	65	theme	crop	260:263	arg1	morphology					271:280	crop stalk morphology	260:280	crop stalk morphology	260:280	There is a strong need for low-cost lignocellulosic composition simultaneous localization methodologies to benefit deeper understandings of crop stalk morphology.					
36184130	7	66	dep	low-cost	1085:1092	arg1	cell-scale					1095:1104	cell-scale	1095:1104	cell-scale	1095:1104	This low-cost, cell-scale method is expected to contribute to new discoveries in many areas of biomass refining and plant science.					
36184130	4	67	theme	channel	635:641	arg1	intensity					650:658	normalized red (R), green (G), and blue (B) channel signal intensity	591:658	normalized red (R), green (G), and blue (B) channel signal intensity	591:658	The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively.					
36184130	7	68	theme	refining	1183:1190	arg1	areas					1166:1170	many areas	1161:1170	many areas of biomass refining and plant science	1161:1208	This low-cost, cell-scale method is expected to contribute to new discoveries in many areas of biomass refining and plant science.					
36184130	1	69	theme	stalk	265:269	arg1	morphology					271:280	crop stalk morphology	260:280	crop stalk morphology	260:280	There is a strong need for low-cost lignocellulosic composition simultaneous localization methodologies to benefit deeper understandings of crop stalk morphology.					
35460710	8	0	theme	resistance	1589:1598	arg1	marker					1600:1605	a methicillin resistance marker	1575:1605	a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator	1575:1661	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	8	0	theme	resistance	1589:1598	arg1	PSM-mec					1566:1572	PSM-mec	1566:1572	PSM-mec	1566:1572	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	8	1	theme	function	1644:1651	arg1	indicator					1653:1661	an agr function indicator	1637:1661	an agr function indicator	1637:1661	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	8	1	theme	function	1644:1651	arg1	delta-toxin					1624:1634	delta-toxin	1624:1634	delta-toxin	1624:1634	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	1	2	theme	bacterial	287:295	arg1	identification					297:310	bacterial identification	287:310	bacterial identification in clinical laboratories	287:335	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	4	3	theme	extract	921:927	arg1	addition					903:910	the addition	899:910	the addition of yeast extract	899:927	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	4	4	theme	aureus	714:719	arg1	43300					733:737	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates	689:770	43300	733:737	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	3	5	theme	phenol-soluble	575:588	arg1	m/z					618:620	m/z 2409	618:625	m/z 2409	618:625	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	3	5	theme	phenol-soluble	575:588	arg1	plates					550:555	various blood agar plates	531:555	various blood agar plates (BAP)	531:561	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	3	5	theme	phenol-soluble	575:588	arg1	peak					612:615	phenol-soluble modulin-mec (PSM-mec) peak	575:615	phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance	575:686	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	1	6	theme	desorption	195:204	arg1	spectrometry					237:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry	173:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS)	173:263	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	1	6	theme	desorption	195:204	arg1	MS					261:262	MALDI-TOF MS	251:262	MALDI-TOF MS	251:262	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	1	7	theme	mass	232:235	arg1	spectrometry					237:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry	173:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS)	173:263	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	1	7	theme	mass	232:235	arg1	MS					261:262	MALDI-TOF MS	251:262	MALDI-TOF MS	251:262	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	2	8	theme	protein	348:354	arg1	expression					356:365	Bacterial protein expression	338:365	Bacterial protein expression	338:365	Bacterial protein expression may differ according to their growth conditions, especially the culture medium composition.					
35460710	2	9	theme	culture	431:437	arg1	composition					446:456	the culture medium composition	427:456	especially the culture medium composition	416:456	Bacterial protein expression may differ according to their growth conditions, especially the culture medium composition.					
35460710	2	9	theme	culture	431:437	arg1	conditions					404:413	their growth conditions	391:413	their growth conditions	391:413	Bacterial protein expression may differ according to their growth conditions, especially the culture medium composition.					
35460710	0	10	theme	various	146:152	arg1	plates					165:170	various blood agar plates	146:170	various blood agar plates	146:170	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	5	11	theme	BAPs	1105:1108	arg1	composition					1090:1100	the composition	1086:1100	the composition of BAPs, especially the presence or absence of yeast extract	1086:1161	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	4	12	theme	clinical	749:756	arg1	isolates					763:770	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates	689:770	isolates	763:770	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	0	13	theme	agar	160:163	arg1	plates					165:170	various blood agar plates	146:170	various blood agar plates	146:170	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	5	14	theme	peaks	956:960	arg1	Analysis					930:937	Analysis	930:937	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs,	930:1000	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	3	15	theme	modulin-mec	590:600	arg1	m/z					618:620	m/z 2409	618:625	m/z 2409	618:625	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	3	15	theme	modulin-mec	590:600	arg1	plates					550:555	various blood agar plates	531:555	various blood agar plates (BAP)	531:561	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	3	15	theme	modulin-mec	590:600	arg1	peak					612:615	phenol-soluble modulin-mec (PSM-mec) peak	575:615	phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance	575:686	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	6	16	theme	low	1217:1219	arg1	intensity					1221:1229	low intensity	1217:1229	low intensity for S. aureus ATCC 43300 and clinical isolates	1217:1276	Especially, the PSM-mec and delta-toxin peaks showed low intensity for S. aureus ATCC 43300 and clinical isolates.					
35460710	6	17	dep	aureus	1238:1243	arg1	ATCC					1245:1248	ATCC 43300	1245:1254	S. aureus ATCC 43300	1235:1254	Especially, the PSM-mec and delta-toxin peaks showed low intensity for S. aureus ATCC 43300 and clinical isolates.					
35460710	0	18	theme	ionization	60:69	arg1	spectrometry					91:102	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	27:102	the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates	23:170	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	3	19	theme	mecA	644:647	arg1	gene					649:652	mecA gene	644:652	mecA gene conferring methicillin resistance	644:686	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	0	20	theme	mass	86:89	arg1	spectrometry					91:102	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	27:102	the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates	23:170	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	5	21	theme	yeast	1149:1153	arg1	extract					1155:1161	yeast extract	1149:1161	yeast extract	1149:1161	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	1	22	theme	Matrix-assisted	173:187	arg1	spectrometry					237:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry	173:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS)	173:263	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	1	22	theme	Matrix-assisted	173:187	arg1	MS					261:262	MALDI-TOF MS	251:262	MALDI-TOF MS	251:262	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	0	23	from	Comparison	0:9	arg1	spectra					104:110	the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra	23:110	the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates	23:170	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	7	24	theme	low-mass	1434:1441	arg1	region					1443:1448	low-mass region	1434:1448	low-mass region (< m/z 4000)	1434:1461	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	7	24	theme	low-mass	1434:1441	arg1	m/z					1453:1455	< m/z 4000	1451:1460	< m/z 4000	1451:1460	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	4	25	theme	tryptic	823:829	arg1	BAPs					846:849	tryptic soy agar-based BAPs	823:849	tryptic soy agar-based BAPs	823:849	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	2	26	theme	medium	439:444	arg1	composition					446:456	the culture medium composition	427:456	especially the culture medium composition	416:456	Bacterial protein expression may differ according to their growth conditions, especially the culture medium composition.					
35460710	2	26	theme	medium	439:444	arg1	conditions					404:413	their growth conditions	391:413	their growth conditions	391:413	Bacterial protein expression may differ according to their growth conditions, especially the culture medium composition.					
35460710	1	27	theme	clinical	315:322	arg1	laboratories					324:335	clinical laboratories	315:335	clinical laboratories	315:335	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	7	28	theme	peaks	1334:1338	arg1	number					1324:1329	the number	1320:1329	the number of peaks	1320:1338	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	4	29	theme	various	789:795	arg1	BAPs					846:849	tryptic soy agar-based BAPs	823:849	tryptic soy agar-based BAPs	823:849	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	4	29	theme	various	789:795	arg1	BAPs					808:811	various commercial BAPs	789:811	various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs	789:892	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	4	29	theme	various	789:795	arg1	BAP					872:874	Columbia agar-based BAP	852:874	Columbia agar-based BAP	852:874	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	4	29	theme	various	789:795	arg1	BAPs					889:892	in-house BAPs	880:892	in-house BAPs	880:892	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	0	30	theme	peaks	14:18	arg1	Comparison					0:9	Comparison	0:9	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.	0:171	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	4	31	theme	ATCC	728:731	arg1	43300					733:737	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates	689:770	43300	733:737	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	4	32	theme	agar-based	861:870	arg1	BAP					872:874	Columbia agar-based BAP	852:874	Columbia agar-based BAP	852:874	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	5	33	theme	weight	1049:1054	arg1	proteins					1056:1063	low-molecular weight proteins	1035:1063	low-molecular weight proteins	1035:1063	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	3	34	theme	agar	545:548	arg1	BAP					558:560	BAP	558:560	BAP	558:560	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	3	34	theme	agar	545:548	arg1	plates					550:555	various blood agar plates	531:555	various blood agar plates (BAP)	531:561	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	3	34	theme	agar	545:548	arg1	peak					612:615	phenol-soluble modulin-mec (PSM-mec) peak	575:615	phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance	575:686	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	0	35	theme	laser	43:47	arg1	spectrometry					91:102	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	27:102	the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates	23:170	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	1	36	used	used	278:281	arg2	MS					261:262	MALDI-TOF MS	251:262	MALDI-TOF MS	251:262	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	1	36	used	used	278:281	arg2	spectrometry					237:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry	173:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS)	173:263	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	4	37	theme	agar-based	835:844	arg1	BAPs					846:849	tryptic soy agar-based BAPs	823:849	tryptic soy agar-based BAPs	823:849	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	3	38	theme	various	531:537	arg1	BAP					558:560	BAP	558:560	BAP	558:560	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	3	38	theme	various	531:537	arg1	plates					550:555	various blood agar plates	531:555	various blood agar plates (BAP)	531:561	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	3	38	theme	various	531:537	arg1	peak					612:615	phenol-soluble modulin-mec (PSM-mec) peak	575:615	phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance	575:686	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	5	39	theme	peak	1015:1018	arg1	intensities					1020:1030	the peak intensities	1011:1030	the peak intensities of low-molecular weight proteins	1011:1063	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	7	40	theme	significant	1282:1292	arg1	differences					1294:1304	No significant differences	1279:1304	No significant differences	1279:1304	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	8	41	theme	methicillin	1577:1587	arg1	marker					1600:1605	a methicillin resistance marker	1575:1605	a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator	1575:1661	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	8	41	theme	methicillin	1577:1587	arg1	PSM-mec					1566:1572	PSM-mec	1566:1572	PSM-mec	1566:1572	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	2	42	theme	Bacterial	338:346	arg1	expression					356:365	Bacterial protein expression	338:365	Bacterial protein expression	338:365	Bacterial protein expression may differ according to their growth conditions, especially the culture medium composition.					
35460710	8	43	theme	agr	1640:1642	arg1	indicator					1653:1661	an agr function indicator	1637:1661	an agr function indicator	1637:1661	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	8	43	theme	agr	1640:1642	arg1	delta-toxin					1624:1634	delta-toxin	1624:1634	delta-toxin	1624:1634	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	7	44	theme	<	1451:1451	arg1	region					1443:1448	low-mass region	1434:1448	low-mass region (< m/z 4000)	1434:1461	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	7	44	theme	<	1451:1451	arg1	m/z					1453:1455	< m/z 4000	1451:1460	< m/z 4000	1451:1460	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	4	45	theme	yeast	915:919	arg1	extract					921:927	yeast extract	915:927	yeast extract	915:927	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	6	46	theme	delta-toxin	1192:1202	arg1	peaks					1204:1208	the PSM-mec and delta-toxin peaks	1176:1208	the PSM-mec and delta-toxin peaks	1176:1208	Especially, the PSM-mec and delta-toxin peaks showed low intensity for S. aureus ATCC 43300 and clinical isolates.					
35460710	2	47	theme	growth	397:402	arg1	composition					446:456	the culture medium composition	427:456	especially the culture medium composition	416:456	Bacterial protein expression may differ according to their growth conditions, especially the culture medium composition.					
35460710	2	47	theme	growth	397:402	arg1	conditions					404:413	their growth conditions	391:413	their growth conditions	391:413	Bacterial protein expression may differ according to their growth conditions, especially the culture medium composition.					
35460710	1	48	theme	laser	189:193	arg1	spectrometry					237:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry	173:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS)	173:263	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	1	48	theme	laser	189:193	arg1	MS					261:262	MALDI-TOF MS	251:262	MALDI-TOF MS	251:262	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	4	49	theme	in-house	880:887	arg1	BAPs					889:892	in-house BAPs	880:892	in-house BAPs	880:892	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	6	50	theme	PSM-mec	1180:1186	arg1	peaks					1204:1208	the PSM-mec and delta-toxin peaks	1176:1208	the PSM-mec and delta-toxin peaks	1176:1208	Especially, the PSM-mec and delta-toxin peaks showed low intensity for S. aureus ATCC 43300 and clinical isolates.					
35460710	6	51	theme	clinical	1260:1267	arg1	isolates					1269:1276	clinical isolates	1260:1276	clinical isolates	1260:1276	Especially, the PSM-mec and delta-toxin peaks showed low intensity for S. aureus ATCC 43300 and clinical isolates.					
35460710	1	52	theme	ionization-time-of-flight	206:230	arg1	spectrometry					237:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry	173:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS)	173:263	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	1	52	theme	ionization-time-of-flight	206:230	arg1	MS					261:262	MALDI-TOF MS	251:262	MALDI-TOF MS	251:262	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	7	53	theme	yeast	1416:1420	arg1	extract					1422:1428	yeast extract	1416:1428	yeast extract	1416:1428	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	1	54	from	identification	297:310	arg1	laboratories					324:335	clinical laboratories	315:335	clinical laboratories	315:335	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	3	55	theme	PSM-mec	603:609	arg1	m/z					618:620	m/z 2409	618:625	m/z 2409	618:625	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	3	55	theme	PSM-mec	603:609	arg1	plates					550:555	various blood agar plates	531:555	various blood agar plates (BAP)	531:561	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	3	55	theme	PSM-mec	603:609	arg1	peak					612:615	phenol-soluble modulin-mec (PSM-mec) peak	575:615	phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance	575:686	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	0	56	theme	blood	154:158	arg1	plates					165:170	various blood agar plates	146:170	various blood agar plates	146:170	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	1	57	theme	MALDI-TOF	251:259	arg1	spectrometry					237:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry	173:248	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS)	173:263	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	1	57	theme	MALDI-TOF	251:259	arg1	MS					261:262	MALDI-TOF MS	251:262	MALDI-TOF MS	251:262	Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories.					
35460710	7	58	located	found	1311:1315	arg2	differences					1294:1304	No significant differences	1279:1304	No significant differences	1279:1304	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	7	58	located	found	1311:1315	arg1	number					1324:1329	the number	1320:1329	the number of peaks	1320:1338	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	5	59	theme	MALDI-TOF	946:954	arg1	peaks					956:960	the MALDI-TOF peaks	942:960	the MALDI-TOF peaks	942:960	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	8	60	theme	tryptic	1478:1484	arg1	agar					1490:1493	tryptic soy agar	1478:1493	tryptic soy agar rather than Columbia agar	1478:1519	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	7	61	theme	lower	1360:1364	arg1	intensity					1366:1374	lower intensity	1360:1374	lower intensity	1360:1374	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	4	62	theme	MRSA	758:761	arg1	isolates					763:770	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates	689:770	isolates	763:770	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	0	63	theme	time-of-flight	71:84	arg1	spectrometry					91:102	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	27:102	the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates	23:170	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	0	64	theme	spectrometry	91:102	arg1	spectra					104:110	the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra	23:110	the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates	23:170	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	8	65	theme	soy	1486:1488	arg1	agar					1490:1493	tryptic soy agar	1478:1493	tryptic soy agar rather than Columbia agar	1478:1519	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	5	66	theme	extract	1155:1161	arg1	presence					1126:1133	presence	1126:1133	presence	1126:1133	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	5	66	theme	extract	1155:1161	arg1	BAPs					1105:1108	BAPs	1105:1108	BAPs	1105:1108	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	5	66	theme	extract	1155:1161	arg1	absence					1138:1144	absence	1138:1144	absence	1138:1144	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	5	67	theme	various	988:994	arg1	BAPs					996:999	various BAPs	988:999	various BAPs	988:999	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	4	68	theme	commercial	797:806	arg1	BAPs					846:849	tryptic soy agar-based BAPs	823:849	tryptic soy agar-based BAPs	823:849	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	4	68	theme	commercial	797:806	arg1	BAPs					808:811	various commercial BAPs	789:811	various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs	789:892	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	4	68	theme	commercial	797:806	arg1	BAP					872:874	Columbia agar-based BAP	852:874	Columbia agar-based BAP	852:874	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	4	68	theme	commercial	797:806	arg1	BAPs					889:892	in-house BAPs	880:892	in-house BAPs	880:892	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	0	69	theme	aureus	130:135	arg1	spectra					104:110	the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra	23:110	the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates	23:170	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	8	70	theme	Columbia	1507:1514	arg1	agar					1516:1519	Columbia agar	1507:1519	tryptic soy agar rather than Columbia agar	1478:1519	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	5	71	theme	aureus	968:973	arg1	peaks					956:960	the MALDI-TOF peaks	942:960	the MALDI-TOF peaks	942:960	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	3	72	theme	peak	481:484	arg1	variations					486:495	the peak variations	477:495	the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance	477:686	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	0	73	theme	matrix-assisted	27:41	arg1	spectrometry					91:102	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	27:102	the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates	23:170	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	7	74	contain	containing	1405:1414	arg1	medium					1398:1403	the medium	1394:1403	the medium containing yeast extract	1394:1428	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	7	74	contain	containing	1405:1414	arg2	extract					1422:1428	yeast extract	1416:1428	yeast extract	1416:1428	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	5	75	theme	low-molecular	1035:1047	arg1	proteins					1056:1063	low-molecular weight proteins	1035:1063	low-molecular weight proteins	1035:1063	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	5	76	dep	presence	1126:1133	arg1	the					1122:1124	the	1122:1124	the	1122:1124	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	3	77	theme	methicillin	665:675	arg1	resistance					677:686	methicillin resistance	665:686	methicillin resistance	665:686	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	4	78	theme	Columbia	852:859	arg1	BAP					872:874	Columbia agar-based BAP	852:874	Columbia agar-based BAP	852:874	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	3	79	theme	aureus	515:520	arg1	variations					486:495	the peak variations	477:495	the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance	477:686	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	0	80	theme	desorption	49:58	arg1	spectrometry					91:102	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	27:102	the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates	23:170	Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates.					
35460710	5	81	theme	proteins	1056:1063	arg1	intensities					1020:1030	the peak intensities	1011:1030	the peak intensities of low-molecular weight proteins	1011:1063	Analysis of the MALDI-TOF peaks of S. aureus, cultured on various BAPs, revealed the peak intensities of low-molecular weight proteins to vary depending on the composition of BAPs, especially the presence or absence of yeast extract.					
35460710	8	82	theme	aureus	1613:1618	arg1	marker					1600:1605	a methicillin resistance marker	1575:1605	a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator	1575:1661	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	8	82	theme	aureus	1613:1618	arg1	PSM-mec					1566:1572	PSM-mec	1566:1572	PSM-mec	1566:1572	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	8	83	theme	PSM-mec	1566:1572	arg1	detection					1553:1561	the detection	1549:1561	the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator	1549:1661	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	4	84	theme	soy	831:833	arg1	BAPs					846:849	tryptic soy agar-based BAPs	823:849	tryptic soy agar-based BAPs	823:849	Methicillin-resistant S. aureus (MRSA) ATCC 43300 and eight clinical MRSA isolates were cultured on various commercial BAPs including tryptic soy agar-based BAPs, Columbia agar-based BAP and in-house BAPs with the addition of yeast extract.					
35460710	3	85	theme	blood	539:543	arg1	BAP					558:560	BAP	558:560	BAP	558:560	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	3	85	theme	blood	539:543	arg1	plates					550:555	various blood agar plates	531:555	various blood agar plates (BAP)	531:561	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	3	85	theme	blood	539:543	arg1	peak					612:615	phenol-soluble modulin-mec (PSM-mec) peak	575:615	phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance	575:686	We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especially phenol-soluble modulin-mec (PSM-mec) peak (m/z 2409) associated with mecA gene conferring methicillin resistance.					
35460710	8	86	theme	delta-toxin	1624:1634	arg1	marker					1600:1605	a methicillin resistance marker	1575:1605	a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator	1575:1661	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	8	86	theme	delta-toxin	1624:1634	arg1	PSM-mec					1566:1572	PSM-mec	1566:1572	PSM-mec	1566:1572	BAPs based on tryptic soy agar rather than Columbia agar seems to be appropriate for the detection of PSM-mec, a methicillin resistance marker of S. aureus and delta-toxin, an agr function indicator.					
35460710	7	87	contain	had	1356:1358	arg1	peaks					1350:1354	some peaks	1345:1354	some peaks	1345:1354	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
35460710	7	87	contain	had	1356:1358	arg2	intensity					1366:1374	lower intensity	1360:1374	lower intensity	1360:1374	No significant differences were found in the number of peaks, but some peaks had lower intensity, corresponding to the medium containing yeast extract, in low-mass region (< m/z 4000).					
36876780	4	0	theme	Clostridium	827:837	arg1	stricto					845:851	Clostridium sensu stricto 1	827:853	Clostridium sensu stricto 1	827:853	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	5	1	theme	In	910:911	arg1	analysis					931:938	In vitro metabolomic analysis	910:938	In vitro metabolomic analysis	910:938	In vitro metabolomic analysis revealed that COS catabolism was associated with significant increases in 3-hydroxybutyrate acid and γ-aminobutyric acid.					
36876780	3	2	theme	normal	672:677	arg1	model					683:687	the normal rat model	668:687	the normal rat model	668:687	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	3	2	theme	normal	672:677	arg1	universal					697:705	universal	697:705	universal	697:705	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	4	3	theme	stricto	845:851	arg1	abundance					814:822	the abundance	810:822	the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs)	810:907	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	5	4	theme	significant	989:999	arg1	increases					1001:1009	significant increases	989:1009	significant increases in 3-hydroxybutyrate acid and γ-aminobutyric acid	989:1059	In vitro metabolomic analysis revealed that COS catabolism was associated with significant increases in 3-hydroxybutyrate acid and γ-aminobutyric acid.					
36876780	5	5	theme	vitro	913:917	arg1	analysis					931:938	In vitro metabolomic analysis	910:938	In vitro metabolomic analysis	910:938	In vitro metabolomic analysis revealed that COS catabolism was associated with significant increases in 3-hydroxybutyrate acid and γ-aminobutyric acid.					
36876780	5	6	theme	COS	954:956	arg1	catabolism					958:967	COS catabolism	954:967	COS catabolism	954:967	In vitro metabolomic analysis revealed that COS catabolism was associated with significant increases in 3-hydroxybutyrate acid and γ-aminobutyric acid.					
36876780	3	7	dep	rats	662:665	arg1	model					683:687	the normal rat model	668:687	the normal rat model	668:687	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	3	7	dep	rats	662:665	arg1	universal					697:705	universal	697:705	universal	697:705	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	0	8	theme	newborn	104:110	arg1	rats					112:115	newborn rats	104:115	newborn rats	104:115	Prebiotic activity of chitooligosaccharides and their ability to alleviate necrotizing enterocolitis in newborn rats.					
36876780	4	9	theme	sensu	839:843	arg1	stricto					845:851	Clostridium sensu stricto 1	827:853	Clostridium sensu stricto 1	827:853	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	4	10	theme	fermentation	722:733	arg1	results					735:741	The in vitro fermentation results	709:741	The in vitro fermentation results	709:741	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	6	11	theme	COS	1112:1114	arg1	potential					1099:1107	the potential	1095:1107	the potential of COS	1095:1114	This study provides evidence for the potential of COS as a prebiotic in food products and to ameliorate NEC development in neonatal rats.					
36876780	1	12	theme	favorable	180:188	arg1	prospects					190:198	favorable prospects	180:198	favorable prospects	180:198	Chitooligosaccharides (COS) have many bioactive functions and favorable prospects in the fields of biomedicine and functional foods.					
36876780	2	13	theme	pathological	506:517	arg1	injury					519:524	intestinal pathological injury	495:524	intestinal pathological injury	495:524	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	6	14	theme	food	1134:1137	arg1	products					1139:1146	food products	1134:1146	food products	1134:1146	This study provides evidence for the potential of COS as a prebiotic in food products and to ameliorate NEC development in neonatal rats.					
36876780	0	15	theme	necrotizing	75:85	arg1	enterocolitis					87:99	necrotizing enterocolitis	75:99	necrotizing enterocolitis in newborn rats	75:115	Prebiotic activity of chitooligosaccharides and their ability to alleviate necrotizing enterocolitis in newborn rats.					
36876780	3	16	from	stricto	624:630	arg1	intestines					641:650	the intestines	637:650	the intestines of normal rats (the normal rat model is more universal)	637:706	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	0	17	theme	Prebiotic	0:8	arg1	activity					10:17	Prebiotic activity	0:17	Prebiotic activity of chitooligosaccharides	0:42	Prebiotic activity of chitooligosaccharides and their ability to alleviate necrotizing enterocolitis in newborn rats.					
36876780	2	18	theme	survival	309:316	arg1	rate					318:321	the survival rate	305:321	the survival rate of neonatal necrotizing enterocolitis (NEC) model rats	305:376	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	2	19	theme	rats	373:376	arg1	rate					318:321	the survival rate	305:321	the survival rate of neonatal necrotizing enterocolitis (NEC) model rats	305:376	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	0	20	theme	chitooligosaccharides	22:42	arg1	ability					54:60	their ability	48:60	their ability	48:60	Prebiotic activity of chitooligosaccharides and their ability to alleviate necrotizing enterocolitis in newborn rats.					
36876780	0	20	theme	chitooligosaccharides	22:42	arg1	activity					10:17	Prebiotic activity	0:17	Prebiotic activity of chitooligosaccharides	0:42	Prebiotic activity of chitooligosaccharides and their ability to alleviate necrotizing enterocolitis in newborn rats.					
36876780	4	21	theme	fatty	889:893	arg1	SCFAs					902:906	SCFAs	902:906	SCFAs	902:906	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	4	21	theme	fatty	889:893	arg1	acids					895:899	produced numerous short-chain fatty acids	859:899	produced numerous short-chain fatty acids (SCFAs)	859:907	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	6	22	theme	neonatal	1185:1192	arg1	rats					1194:1197	neonatal rats	1185:1197	neonatal rats	1185:1197	This study provides evidence for the potential of COS as a prebiotic in food products and to ameliorate NEC development in neonatal rats.					
36876780	5	23	theme	metabolomic	919:929	arg1	analysis					931:938	In vitro metabolomic analysis	910:938	In vitro metabolomic analysis	910:938	In vitro metabolomic analysis revealed that COS catabolism was associated with significant increases in 3-hydroxybutyrate acid and γ-aminobutyric acid.					
36876780	4	24	theme	human	778:782	arg1	microbiota					788:797	the human gut microbiota	774:797	the human gut microbiota	774:797	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	2	25	theme	intestinal	408:417	arg1	microbiota					419:428	the intestinal microbiota	404:428	the intestinal microbiota	404:428	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	2	26	theme	microbiota	419:428	arg1	composition					389:399	the composition	385:399	the composition of the intestinal microbiota	385:428	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	4	27	theme	short-chain	877:887	arg1	SCFAs					902:906	SCFAs	902:906	SCFAs	902:906	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	4	27	theme	short-chain	877:887	arg1	acids					895:899	produced numerous short-chain fatty acids	859:899	produced numerous short-chain fatty acids (SCFAs)	859:907	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	5	28	theme	γ-aminobutyric	1041:1054	arg1	acid					1056:1059	γ-aminobutyric acid	1041:1059	γ-aminobutyric acid	1041:1059	In vitro metabolomic analysis revealed that COS catabolism was associated with significant increases in 3-hydroxybutyrate acid and γ-aminobutyric acid.					
36876780	4	29	theme	in	713:714	arg1	results					735:741	The in vitro fermentation results	709:741	The in vitro fermentation results	709:741	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	1	30	theme	many	151:154	arg1	functions					166:174	many bioactive functions	151:174	many bioactive functions	151:174	Chitooligosaccharides (COS) have many bioactive functions and favorable prospects in the fields of biomedicine and functional foods.					
36876780	2	31	theme	cytokines	470:478	arg1	expression					443:452	the expression	439:452	the expression of inflammatory cytokines	439:478	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	4	32	dep	in	713:714	arg1	vitro					716:720	vitro	716:720	vitro	716:720	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	0	33	from	enterocolitis	87:99	arg1	rats					112:115	newborn rats	104:115	newborn rats	104:115	Prebiotic activity of chitooligosaccharides and their ability to alleviate necrotizing enterocolitis in newborn rats.					
36876780	3	34	theme	Bacteroides	589:599	arg1	abundance					563:571	the abundance	559:571	the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal)	559:706	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	6	35	from	prebiotic	1121:1129	arg1	products					1139:1146	food products	1134:1146	food products	1134:1146	This study provides evidence for the potential of COS as a prebiotic in food products and to ameliorate NEC development in neonatal rats.					
36876780	1	36	theme	biomedicine	217:227	arg1	fields					207:212	the fields	203:212	the fields of biomedicine and functional foods	203:248	Chitooligosaccharides (COS) have many bioactive functions and favorable prospects in the fields of biomedicine and functional foods.					
36876780	2	37	theme	inflammatory	457:468	arg1	cytokines					470:478	inflammatory cytokines	457:478	inflammatory cytokines	457:478	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	4	38	theme	acids	895:899	arg1	abundance					814:822	the abundance	810:822	the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs)	810:907	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	3	39	from	intestines	641:650	arg1	abundance					563:571	the abundance	559:571	the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal)	559:706	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	3	40	theme	Akkermansia	576:586	arg1	abundance					563:571	the abundance	559:571	the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal)	559:706	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	2	41	theme	enterocolitis	347:359	arg1	rats					373:376	neonatal necrotizing enterocolitis (NEC) model rats	326:376	neonatal necrotizing enterocolitis (NEC) model rats	326:376	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	3	42	theme	rat	679:681	arg1	model					683:687	the normal rat model	668:687	the normal rat model	668:687	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	3	42	theme	rat	679:681	arg1	universal					697:705	universal	697:705	universal	697:705	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	3	43	theme	rats	662:665	arg1	intestines					641:650	the intestines	637:650	the intestines of normal rats (the normal rat model is more universal)	637:706	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	4	44	theme	gut	784:786	arg1	microbiota					788:797	the human gut microbiota	774:797	the human gut microbiota	774:797	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	2	45	theme	necrotizing	335:345	arg1	NEC					362:364	NEC	362:364	NEC	362:364	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	2	45	theme	necrotizing	335:345	arg1	enterocolitis					347:359	necrotizing enterocolitis	335:359	neonatal necrotizing enterocolitis (NEC) model rats	326:376	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	2	46	located	found	274:278	arg2	COS					266:268	COS	266:268	COS	266:268	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	2	46	located	found	274:278	arg1	study					259:263	this study	254:263	this study	254:263	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	4	47	theme	numerous	868:875	arg1	SCFAs					902:906	SCFAs	902:906	SCFAs	902:906	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	4	47	theme	numerous	868:875	arg1	acids					895:899	produced numerous short-chain fatty acids	859:899	produced numerous short-chain fatty acids (SCFAs)	859:907	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	5	48	theme	3-hydroxybutyrate	1014:1030	arg1	acid					1032:1035	3-hydroxybutyrate acid	1014:1035	3-hydroxybutyrate acid	1014:1035	In vitro metabolomic analysis revealed that COS catabolism was associated with significant increases in 3-hydroxybutyrate acid and γ-aminobutyric acid.					
36876780	3	49	theme	sensu	618:622	arg1	stricto					624:630	Clostridium sensu stricto 1	606:632	Clostridium sensu stricto 1	606:632	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	2	50	theme	neonatal	326:333	arg1	rats					373:376	neonatal necrotizing enterocolitis (NEC) model rats	326:376	neonatal necrotizing enterocolitis (NEC) model rats	326:376	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	1	51	theme	functional	233:242	arg1	foods					244:248	functional foods	233:248	functional foods	233:248	Chitooligosaccharides (COS) have many bioactive functions and favorable prospects in the fields of biomedicine and functional foods.					
36876780	4	52	theme	produced	859:866	arg1	SCFAs					902:906	SCFAs	902:906	SCFAs	902:906	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	4	52	theme	produced	859:866	arg1	acids					895:899	produced numerous short-chain fatty acids	859:899	produced numerous short-chain fatty acids (SCFAs)	859:907	The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs).					
36876780	3	53	theme	stricto	624:630	arg1	abundance					563:571	the abundance	559:571	the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal)	559:706	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	5	54	from	increases	1001:1009	arg1	acid					1032:1035	3-hydroxybutyrate acid	1014:1035	3-hydroxybutyrate acid	1014:1035	In vitro metabolomic analysis revealed that COS catabolism was associated with significant increases in 3-hydroxybutyrate acid and γ-aminobutyric acid.					
36876780	5	54	from	increases	1001:1009	arg1	acid					1056:1059	γ-aminobutyric acid	1041:1059	γ-aminobutyric acid	1041:1059	In vitro metabolomic analysis revealed that COS catabolism was associated with significant increases in 3-hydroxybutyrate acid and γ-aminobutyric acid.					
36876780	3	55	from	abundance	563:571	arg1	intestines					641:650	the intestines	637:650	the intestines of normal rats (the normal rat model is more universal)	637:706	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	1	56	theme	foods	244:248	arg1	fields					207:212	the fields	203:212	the fields of biomedicine and functional foods	203:248	Chitooligosaccharides (COS) have many bioactive functions and favorable prospects in the fields of biomedicine and functional foods.					
36876780	2	57	theme	intestinal	495:504	arg1	injury					519:524	intestinal pathological injury	495:524	intestinal pathological injury	495:524	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	3	58	theme	normal	655:660	arg1	rats					662:665	normal rats	655:665	normal rats (the normal rat model is more universal)	655:706	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	6	59	theme	NEC	1166:1168	arg1	development					1170:1180	NEC development	1166:1180	NEC development	1166:1180	This study provides evidence for the potential of COS as a prebiotic in food products and to ameliorate NEC development in neonatal rats.					
36876780	2	60	theme	model	367:371	arg1	rats					373:376	neonatal necrotizing enterocolitis (NEC) model rats	326:376	neonatal necrotizing enterocolitis (NEC) model rats	326:376	In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury.					
36876780	1	61	contain	have	146:149	arg2	functions					166:174	many bioactive functions	151:174	many bioactive functions	151:174	Chitooligosaccharides (COS) have many bioactive functions and favorable prospects in the fields of biomedicine and functional foods.					
36876780	1	61	contain	have	146:149	arg1	COS					141:143	COS	141:143	COS	141:143	Chitooligosaccharides (COS) have many bioactive functions and favorable prospects in the fields of biomedicine and functional foods.					
36876780	1	61	contain	have	146:149	arg2	prospects					190:198	favorable prospects	180:198	favorable prospects	180:198	Chitooligosaccharides (COS) have many bioactive functions and favorable prospects in the fields of biomedicine and functional foods.					
36876780	1	61	contain	have	146:149	arg1	Chitooligosaccharides					118:138	Chitooligosaccharides	118:138	Chitooligosaccharides (COS)	118:144	Chitooligosaccharides (COS) have many bioactive functions and favorable prospects in the fields of biomedicine and functional foods.					
36876780	3	62	theme	Clostridium	606:616	arg1	stricto					624:630	Clostridium sensu stricto 1	606:632	Clostridium sensu stricto 1	606:632	In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal).					
36876780	1	63	theme	bioactive	156:164	arg1	functions					166:174	many bioactive functions	151:174	many bioactive functions	151:174	Chitooligosaccharides (COS) have many bioactive functions and favorable prospects in the fields of biomedicine and functional foods.					
36336156	6	0	theme	Burkholderia	1285:1296	arg1	sp					1298:1299	Burkholderia sp	1285:1299	Burkholderia sp	1285:1299	LIM05 and Burkholderia sp.					
36336156	2	1	theme	31 wt	721:725	arg1	%					726:726	31 wt%	721:726	31 wt%	721:726	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	3	2	theme	most	882:885	arg1	conditions					903:912	the most severe reaction conditions	878:912	the most severe reaction conditions	878:912	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	0	3	theme	sugarcane	90:98	arg1	bagasse					100:106	sugarcane bagasse	90:106	sugarcane bagasse	90:106	Exploring the compatibility between hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria.					
36336156	1	4	theme	produced	356:363	arg1	bio-oils					372:379	the produced lignin bio-oils	352:379	the produced lignin bio-oils	352:379	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	2	5	theme	time	634:637	arg1	influence					595:603	the influence	591:603	the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils)	591:761	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	4	6	theme	distinct	1228:1235	arg1	species					1237:1243	9 distinct species	1226:1243	9 distinct species	1226:1243	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	5	7	theme	Pseudomonas	1259:1269	arg1	sp					1271:1272	Pseudomonas sp	1259:1272	Pseudomonas sp	1259:1272	The isolates Pseudomonas sp.					
36336156	1	8	theme	isolated	282:289	arg1	lignins					291:297	isolated lignins	282:297	isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils	282:379	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	1	9	theme	lignin	365:370	arg1	bio-oils					372:379	the produced lignin bio-oils	352:379	the produced lignin bio-oils	352:379	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	3	10	from	pronounced	864:873	arg1	conditions					903:912	the most severe reaction conditions	878:912	the most severe reaction conditions	878:912	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	4	11	theme	rich	1163:1166	arg1	bio-oil					1155:1161	the corresponding light bio-oil	1131:1161	the corresponding light bio-oil rich in aromatic monomers	1131:1187	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	1	12	theme	lignins	291:297	arg1	depolymerization					262:277	the depolymerization	258:277	the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion	258:398	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	2	13	theme	alkaline	553:560	arg1	fractionation					562:574	alkaline fractionation	553:574	alkaline fractionation	553:574	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	1	14	theme	bio-oils	372:379	arg1	yield					311:315	yield	311:315	yield	311:315	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	1	14	theme	bio-oils	372:379	arg1	composition					318:328	composition	318:328	composition	318:328	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	1	14	theme	bio-oils	372:379	arg1	compatibility					335:347	compatibility	335:347	compatibility	335:347	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	4	15	theme	aromatic	1171:1178	arg1	monomers					1180:1187	aromatic monomers	1171:1187	aromatic monomers	1171:1187	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	2	16	theme	ratio	669:673	arg1	influence					595:603	the influence	591:603	the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils)	591:761	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	4	17	from	species	1237:1243	arg1	bacteria					1212:1219	bacteria	1212:1219	bacteria from 9 distinct species	1212:1243	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	4	17	from	species	1237:1243	arg1	growth					1202:1207	the growth	1198:1207	the growth of bacteria from 9 distinct species	1198:1243	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	2	18	theme	light	747:751	arg1	monomers					737:744	monomers	737:744	monomers (light bio-oils)	737:761	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	2	18	theme	light	747:751	arg1	bio-oils					753:760	light bio-oils	747:760	light bio-oils	747:760	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	2	19	dep	%	726:726	arg1	up					715:716	up	715:716	up	715:716	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	2	20	theme	solid-liquid	656:667	arg1	1:10-1:50 m.v-1					676:690	1:10-1:50 m.v-1	676:690	1:10-1:50 m.v-1	676:690	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	2	20	theme	solid-liquid	656:667	arg1	ratio					669:673	solid-liquid ratio	656:673	solid-liquid ratio (1:10-1:50 m.v-1)	656:691	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	7	21	theme	future	1419:1424	arg1	development					1426:1436	the future development	1415:1436	the future development of biological upgrading strategies tailored for this lignin stream	1415:1503	LIM09 showed the best growth performance and tolerance to lignin-derived aromatics, being the most promising for the future development of biological upgrading strategies tailored for this lignin stream.					
36336156	1	22	theme	biomass	194:200	arg1	fractionation					202:214	biomass fractionation	194:214	biomass fractionation	194:214	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	7	23	theme	upgrading	1452:1460	arg1	strategies					1462:1471	biological upgrading strategies	1441:1471	biological upgrading strategies tailored for this lignin stream	1441:1503	LIM09 showed the best growth performance and tolerance to lignin-derived aromatics, being the most promising for the future development of biological upgrading strategies tailored for this lignin stream.					
36336156	4	24	theme	heavy	1036:1040	arg1	bio-oil					1043:1049	the high-molecular-weight (heavy) bio-oil	1009:1049	the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1	1009:1093	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	3	25	theme	severe	887:892	arg1	conditions					903:912	the most severe reaction conditions	878:912	the most severe reaction conditions	878:912	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	2	26	theme	hydrothermal	462:473	arg1	depolymerization					475:490	the hydrothermal depolymerization	458:490	the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse	458:548	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	2	27	theme	lignin	510:515	arg1	depolymerization					475:490	the hydrothermal depolymerization	458:490	the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse	458:548	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	1	28	from	terms	302:306	arg1	depolymerization					262:277	the depolymerization	258:277	the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion	258:398	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	5	29	dep	sp	1271:1272	arg1	isolates					1250:1257	The isolates	1246:1257	The isolates	1246:1257	The isolates Pseudomonas sp.					
36336156	3	30	theme	reaction	894:901	arg1	conditions					903:912	the most severe reaction conditions	878:912	the most severe reaction conditions	878:912	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	4	31	theme	high-molecular-weight	1013:1033	arg1	bio-oil					1043:1049	the high-molecular-weight (heavy) bio-oil	1009:1049	the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1	1009:1093	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	7	32	theme	best	1319:1322	arg1	performance					1331:1341	growth performance	1324:1341	growth performance	1324:1341	LIM09 showed the best growth performance and tolerance to lignin-derived aromatics, being the most promising for the future development of biological upgrading strategies tailored for this lignin stream.					
36336156	7	33	theme	strategies	1462:1471	arg1	development					1426:1436	the future development	1415:1436	the future development of biological upgrading strategies tailored for this lignin stream	1415:1503	LIM09 showed the best growth performance and tolerance to lignin-derived aromatics, being the most promising for the future development of biological upgrading strategies tailored for this lignin stream.					
36336156	1	34	from	lignins	291:297	arg1	terms					302:306	terms	302:306	terms of yield, composition, and compatibility of the produced lignin bio-oils	302:379	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	4	35	theme	bacteria	1212:1219	arg1	growth					1202:1207	the growth	1198:1207	the growth of bacteria from 9 distinct species	1198:1243	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	4	36	theme	light	1149:1153	arg1	bio-oil					1155:1161	the corresponding light bio-oil	1131:1161	the corresponding light bio-oil rich in aromatic monomers	1131:1187	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	1	37	theme	yield	311:315	arg1	terms					302:306	terms	302:306	terms of yield, composition, and compatibility of the produced lignin bio-oils	302:379	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	0	38	theme	aromatics	134:142	arg1	metabolization					112:125	metabolization	112:125	metabolization of the aromatics by bacteria	112:154	Exploring the compatibility between hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria.					
36336156	0	38	theme	aromatics	134:142	arg1	bagasse					100:106	sugarcane bagasse	90:106	sugarcane bagasse	90:106	Exploring the compatibility between hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria.					
36336156	0	39	from	bagasse	100:106	arg1	depolymerization					49:64	hydrothermal depolymerization	36:64	hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria	36:154	Exploring the compatibility between hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria.					
36336156	4	40	from	growth	1202:1207	arg1	species					1237:1243	9 distinct species	1226:1243	9 distinct species	1226:1243	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	4	41	theme	bacterial	1113:1121	arg1	growth					1123:1128	bacterial growth	1113:1128	bacterial growth	1113:1128	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	7	42	theme	lignin-derived	1360:1373	arg1	aromatics					1375:1383	lignin-derived aromatics	1360:1383	lignin-derived aromatics	1360:1383	LIM09 showed the best growth performance and tolerance to lignin-derived aromatics, being the most promising for the future development of biological upgrading strategies tailored for this lignin stream.					
36336156	2	43	theme	β-O-4'-rich	498:508	arg1	lignin					510:515	an β-O-4'-rich lignin	495:515	an β-O-4'-rich lignin extracted from sugarcane bagasse	495:548	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	2	44	from	monomers	737:744	arg1	rich					729:732	rich	729:732	rich	729:732	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	2	45	from	rich	729:732	arg1	monomers					737:744	monomers	737:744	monomers (light bio-oils)	737:761	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	2	45	from	rich	729:732	arg1	bio-oils					753:760	light bio-oils	747:760	light bio-oils	747:760	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	0	46	theme	hydrothermal	36:47	arg1	depolymerization					49:64	hydrothermal depolymerization	36:64	hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria	36:154	Exploring the compatibility between hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria.					
36336156	1	47	theme	composition	318:328	arg1	terms					302:306	terms	302:306	terms of yield, composition, and compatibility of the produced lignin bio-oils	302:379	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	7	48	theme	growth	1324:1329	arg1	performance					1331:1341	growth performance	1324:1341	growth performance	1324:1341	LIM09 showed the best growth performance and tolerance to lignin-derived aromatics, being the most promising for the future development of biological upgrading strategies tailored for this lignin stream.					
36336156	3	49	theme	Components	774:783	arg1	Analysis					785:792	Principal Components Analysis	764:792	Principal Components Analysis	764:792	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	3	50	theme	monomers	957:964	arg1	abundance					927:935	the abundance	923:935	the abundance of more hydrophobic monomers	923:964	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	0	51	theme	alkaline	69:76	arg1	lignin					78:83	alkaline lignin	69:83	alkaline lignin	69:83	Exploring the compatibility between hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria.					
36336156	3	52	theme	Principal	764:772	arg1	Analysis					785:792	Principal Components Analysis	764:792	Principal Components Analysis	764:792	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	4	53	from	monomers	1180:1187	arg1	rich					1163:1166	rich	1163:1166	rich	1163:1166	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	2	54	theme	temperature	608:618	arg1	influence					595:603	the influence	591:603	the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils)	591:761	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	7	55	theme	lignin	1491:1496	arg1	stream					1498:1503	this lignin stream	1486:1503	this lignin stream	1486:1503	LIM09 showed the best growth performance and tolerance to lignin-derived aromatics, being the most promising for the future development of biological upgrading strategies tailored for this lignin stream.					
36336156	1	56	from	depolymerization	262:277	arg1	terms					302:306	terms	302:306	terms of yield, composition, and compatibility of the produced lignin bio-oils	302:379	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	7	57	dep	performance	1331:1341	arg1	the					1315:1317	the	1315:1317	the	1315:1317	LIM09 showed the best growth performance and tolerance to lignin-derived aromatics, being the most promising for the future development of biological upgrading strategies tailored for this lignin stream.					
36336156	1	58	theme	compatibility	335:347	arg1	terms					302:306	terms	302:306	terms of yield, composition, and compatibility of the produced lignin bio-oils	302:379	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	1	59	from	effect	248:253	arg1	depolymerization					262:277	the depolymerization	258:277	the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion	258:398	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	2	60	theme	bio-oils	705:712	arg1	yield					696:700	yield	696:700	yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils)	696:761	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	3	61	theme	hydrophobic	945:955	arg1	monomers					957:964	more hydrophobic monomers	940:964	more hydrophobic monomers	940:964	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	0	62	from	metabolization	112:125	arg1	depolymerization					49:64	hydrothermal depolymerization	36:64	hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria	36:154	Exploring the compatibility between hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria.					
36336156	4	63	from	rich	1163:1166	arg1	monomers					1180:1187	aromatic monomers	1171:1187	aromatic monomers	1171:1187	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	2	64	theme	rich	729:732	arg1	bio-oils					705:712	bio-oils	705:712	bio-oils (up to 31 wt%) rich in monomers (light bio-oils)	705:761	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	4	65	theme	corresponding	1135:1147	arg1	bio-oil					1155:1161	the corresponding light bio-oil	1131:1161	the corresponding light bio-oil rich in aromatic monomers	1131:1187	While the high-molecular-weight (heavy) bio-oil generated at 350 °C, 90 min, and 1:50 m.v-1 failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species.					
36336156	3	66	theme	aromatic	837:844	arg1	monomers					846:853	the aromatic monomers	833:853	the aromatic monomers	833:853	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	3	67	theme	diluted	984:990	arg1	reactions					992:1000	more diluted reactions	979:1000	more diluted reactions	979:1000	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	2	68	theme	sugarcane	532:540	arg1	bagasse					542:548	sugarcane bagasse	532:548	sugarcane bagasse	532:548	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	7	69	theme	biological	1441:1450	arg1	strategies					1462:1471	biological upgrading strategies	1441:1471	biological upgrading strategies tailored for this lignin stream	1441:1503	LIM09 showed the best growth performance and tolerance to lignin-derived aromatics, being the most promising for the future development of biological upgrading strategies tailored for this lignin stream.					
36336156	3	70	from	conditions	903:912	arg1	defunctionalization					810:828	the defunctionalization	806:828	the defunctionalization of the aromatic monomers	806:853	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	3	70	from	conditions	903:912	arg1	pronounced					864:873	pronounced	864:873	pronounced	864:873	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	1	71	with	depolymerization	262:277	arg1	bioconversion					386:398	bioconversion	386:398	bioconversion	386:398	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
36336156	3	72	theme	monomers	846:853	arg1	defunctionalization					810:828	the defunctionalization	806:828	the defunctionalization of the aromatic monomers	806:853	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	3	72	theme	monomers	846:853	arg1	pronounced					864:873	pronounced	864:873	pronounced	864:873	Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions.					
36336156	2	73	from	influence	595:603	arg1	yield					696:700	yield	696:700	yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils)	696:761	In this study, we evaluated the hydrothermal depolymerization of an β-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 °C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v-1) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils).					
36336156	0	74	theme	lignin	78:83	arg1	depolymerization					49:64	hydrothermal depolymerization	36:64	hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria	36:154	Exploring the compatibility between hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria.					
36336156	7	75	link	lignin-derived	1360:1373	arg1	aromatics					1375:1383	lignin-derived aromatics	1360:1383	lignin-derived aromatics	1360:1383	LIM09 showed the best growth performance and tolerance to lignin-derived aromatics, being the most promising for the future development of biological upgrading strategies tailored for this lignin stream.					
36336156	1	76	theme	hydrothermal	166:177	arg1	treatments					179:188	hydrothermal treatments	166:188	hydrothermal treatments for biomass fractionation	166:214	Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated.					
37044324	4	0	theme	good	968:971	arg1	survival					1007:1014	high cell survival	997:1014	high cell survival	997:1014	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	4	0	theme	good	968:971	arg1	activity					987:994	good mitochondrial activity	968:994	good mitochondrial activity	968:994	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	3	1	theme	1005 ± 174 KPa	773:786	arg1	ratio					689:693	a swelling ratio	678:693	a swelling ratio of 1278 % ± 270 %	678:711	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	3	1	theme	1005 ± 174 KPa	773:786	arg1	strength					761:768	a compressive strength	747:768	a compressive strength of 1005 ± 174 KPa	747:786	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	1	2	theme	extracellular	326:338	arg1	components					340:349	native cartilaginous extracellular components	305:349	native cartilaginous extracellular components	305:349	The microenvironment plays a crucial role in stem cell differentiation, and a scaffold that mimics native cartilaginous extracellular components can promote chondrogenesis.					
37044324	6	3	theme	SCID	1319:1322	arg1	mice					1324:1327	SCID mice	1319:1327	SCID mice	1319:1327	Ex vivo study revealed that the SDSC-tetra-copolymer scaffold regenerated cartilage-like tissue in SCID mice with abundant type II collagen and S-100 production.					
37044324	7	4	from	expression	1398:1407	arg1	group					1441:1445	the tetra-copolymer scaffold group	1412:1445	the tetra-copolymer scaffold group	1412:1445	BMP7 and COL2A1 expression in the tetra-copolymer scaffold group was much higher than that in the gelatin scaffold group ex vivo.					
37044324	3	5	theme	1278 	698:702	arg1	%					703:703	%	703:703	%	703:703	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	1	6	theme	cell	256:259	arg1	differentiation					261:275	stem cell differentiation	251:275	stem cell differentiation	251:275	The microenvironment plays a crucial role in stem cell differentiation, and a scaffold that mimics native cartilaginous extracellular components can promote chondrogenesis.					
37044324	6	7	theme	SDSC-tetra-copolymer	1252:1271	arg1	scaffold					1273:1280	the SDSC-tetra-copolymer scaffold	1248:1280	the SDSC-tetra-copolymer scaffold	1248:1280	Ex vivo study revealed that the SDSC-tetra-copolymer scaffold regenerated cartilage-like tissue in SCID mice with abundant type II collagen and S-100 production.					
37044324	3	8	theme	compression	826:836	arg1	force					838:842	compression force	826:842	compression force	826:842	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	0	9	theme	stem	194:197	arg1	cells					199:203	human synovium-derived stem cells	171:203	human synovium-derived stem cells	171:203	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	5	10	theme	Nrf2	1195:1198	arg1	expression					1166:1175	upregulated mRNA expression	1149:1175	upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7	1149:1217	SDSCs cultured on the tetra-copolymer scaffold with chondrogenic induction exhibited upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7.					
37044324	4	11	theme	glycosaminoglycan	1034:1050	arg1	production					1052:1061	a pronounced glycosaminoglycan production	1021:1061	a pronounced glycosaminoglycan production	1021:1061	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	6	12	theme	S-100	1364:1368	arg1	production					1370:1379	S-100 production	1364:1379	S-100 production	1364:1379	Ex vivo study revealed that the SDSC-tetra-copolymer scaffold regenerated cartilage-like tissue in SCID mice with abundant type II collagen and S-100 production.					
37044324	5	13	theme	TGF-β1	1201:1206	arg1	expression					1166:1175	upregulated mRNA expression	1149:1175	upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7	1149:1217	SDSCs cultured on the tetra-copolymer scaffold with chondrogenic induction exhibited upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7.					
37044324	4	14	theme	high	997:1000	arg1	survival					1007:1014	high cell survival	997:1014	high cell survival	997:1014	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	4	14	theme	high	997:1000	arg1	activity					987:994	good mitochondrial activity	968:994	good mitochondrial activity	968:994	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	5	15	theme	COL2A1	1180:1185	arg1	expression					1166:1175	upregulated mRNA expression	1149:1175	upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7	1149:1217	SDSCs cultured on the tetra-copolymer scaffold with chondrogenic induction exhibited upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7.					
37044324	3	16	theme	 ± 270 	704:710	arg1	%					703:703	%	703:703	%	703:703	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	8	17	theme	tissue	1616:1621	arg1	engineering					1623:1633	cartilage tissue engineering	1606:1633	cartilage tissue engineering	1606:1633	The tetra-copolymer scaffold thus exhibits strong chondrogenic capability and will facilitate cartilage tissue engineering.					
37044324	6	18	theme	type	1343:1346	arg1	collagen					1351:1358	abundant type II collagen	1334:1358	abundant type II collagen	1334:1358	Ex vivo study revealed that the SDSC-tetra-copolymer scaffold regenerated cartilage-like tissue in SCID mice with abundant type II collagen and S-100 production.					
37044324	5	19	theme	ChM-1	1188:1192	arg1	expression					1166:1175	upregulated mRNA expression	1149:1175	upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7	1149:1217	SDSCs cultured on the tetra-copolymer scaffold with chondrogenic induction exhibited upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7.					
37044324	3	20	theme	77.68 	728:733	arg1	%					734:734	%	734:734	%	734:734	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	4	21	attach	attached	922:929	arg2	scaffold					913:920	the tetra-copolymer scaffold	893:920	the tetra-copolymer scaffold attached to the scaffold firmly	893:952	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	4	21	attach	attached	922:929	arg1	scaffold					938:945	the scaffold	934:945	the scaffold	934:945	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	0	22	theme	cartilage	110:118	arg1	matrix					134:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	2	23	theme	pure	612:615	arg1	scaffold					625:632	a pure gelatin scaffold	610:632	a pure gelatin scaffold	610:632	In this study, a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage was fabricated using a microfluidic technique and compared with a pure gelatin scaffold.					
37044324	1	24	theme	crucial	235:241	arg1	role					243:246	a crucial role	233:246	a crucial role	233:246	The microenvironment plays a crucial role in stem cell differentiation, and a scaffold that mimics native cartilaginous extracellular components can promote chondrogenesis.					
37044324	6	25	theme	abundant	1334:1341	arg1	collagen					1351:1358	abundant type II collagen	1334:1358	abundant type II collagen	1334:1358	Ex vivo study revealed that the SDSC-tetra-copolymer scaffold regenerated cartilage-like tissue in SCID mice with abundant type II collagen and S-100 production.					
37044324	0	26	theme	human	171:175	arg1	cells					199:203	human synovium-derived stem cells	171:203	human synovium-derived stem cells	171:203	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	2	27	theme	microfluidic	569:580	arg1	technique					582:590	a microfluidic technique	567:590	a microfluidic technique	567:590	In this study, a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage was fabricated using a microfluidic technique and compared with a pure gelatin scaffold.					
37044324	2	28	theme	similar	508:514	arg1	composition					479:489	composition	479:489	composition	479:489	In this study, a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage was fabricated using a microfluidic technique and compared with a pure gelatin scaffold.					
37044324	0	29	theme	Bioinspired	0:10	arg1	matrix					134:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	4	30	theme	Synovium-derived	845:860	arg1	cells					867:871	Synovium-derived stem cells	845:871	Synovium-derived stem cells (SDSCs)	845:879	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	4	30	theme	Synovium-derived	845:860	arg1	SDSCs					874:878	SDSCs	874:878	SDSCs	874:878	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	0	31	theme	acid-chondroitin	40:55	arg1	matrix					134:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	8	32	theme	tetra-copolymer	1516:1530	arg1	scaffold					1532:1539	The tetra-copolymer scaffold	1512:1539	The tetra-copolymer scaffold	1512:1539	The tetra-copolymer scaffold thus exhibits strong chondrogenic capability and will facilitate cartilage tissue engineering.					
37044324	2	33	theme	hyaline	528:534	arg1	cartilage					536:544	hyaline cartilage	528:544	hyaline cartilage	528:544	In this study, a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage was fabricated using a microfluidic technique and compared with a pure gelatin scaffold.					
37044324	0	34	theme	tetra-copolymer	65:79	arg1	matrix					134:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	5	35	theme	mRNA	1161:1164	arg1	expression					1166:1175	upregulated mRNA expression	1149:1175	upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7	1149:1217	SDSCs cultured on the tetra-copolymer scaffold with chondrogenic induction exhibited upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7.					
37044324	7	36	theme	scaffold	1432:1439	arg1	group					1441:1445	the tetra-copolymer scaffold group	1412:1445	the tetra-copolymer scaffold group	1412:1445	BMP7 and COL2A1 expression in the tetra-copolymer scaffold group was much higher than that in the gelatin scaffold group ex vivo.					
37044324	3	37	theme	 ± 11.70 	735:743	arg1	%					734:734	%	734:734	%	734:734	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	6	38	dep	Ex	1220:1221	arg1	vivo					1223:1226	vivo	1223:1226	vivo	1223:1226	Ex vivo study revealed that the SDSC-tetra-copolymer scaffold regenerated cartilage-like tissue in SCID mice with abundant type II collagen and S-100 production.					
37044324	2	39	theme	tetra-copolymer	449:463	arg1	scaffold					465:472	a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold	394:472	a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage	394:544	In this study, a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage was fabricated using a microfluidic technique and compared with a pure gelatin scaffold.					
37044324	0	40	theme	biomimicking	90:101	arg1	matrix					134:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	3	41	contain	had	674:676	arg2	ratio					689:693	a swelling ratio	678:693	a swelling ratio of 1278 % ± 270 %	678:711	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	3	41	contain	had	674:676	arg2	strength					761:768	a compressive strength	747:768	a compressive strength of 1005 ± 174 KPa	747:786	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	3	41	contain	had	674:676	arg1	scaffold					665:672	The newly designed biomimetic scaffold	635:672	The newly designed biomimetic scaffold	635:672	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	3	41	contain	had	674:676	arg2	porosity					716:723	a porosity	714:723	a porosity of 77.68 % ± 11.70 %	714:744	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	8	42	theme	strong	1555:1560	arg1	capability					1575:1584	strong chondrogenic capability	1555:1584	strong chondrogenic capability	1555:1584	The tetra-copolymer scaffold thus exhibits strong chondrogenic capability and will facilitate cartilage tissue engineering.					
37044324	2	43	theme	acid-chondroitin	424:439	arg1	scaffold					465:472	a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold	394:472	a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage	394:544	In this study, a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage was fabricated using a microfluidic technique and compared with a pure gelatin scaffold.					
37044324	5	44	theme	chondrogenic	1116:1127	arg1	induction					1129:1137	chondrogenic induction	1116:1137	chondrogenic induction	1116:1137	SDSCs cultured on the tetra-copolymer scaffold with chondrogenic induction exhibited upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7.					
37044324	0	45	link	synovium-derived	177:192	arg1	cells					199:203	human synovium-derived stem cells	171:203	human synovium-derived stem cells	171:203	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	3	46	theme	compressive	749:759	arg1	ratio					689:693	a swelling ratio	678:693	a swelling ratio of 1278 % ± 270 %	678:711	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	3	46	theme	compressive	749:759	arg1	strength					761:768	a compressive strength	747:768	a compressive strength of 1005 ± 174 KPa	747:786	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	1	47	theme	cartilaginous	312:324	arg1	components					340:349	native cartilaginous extracellular components	305:349	native cartilaginous extracellular components	305:349	The microenvironment plays a crucial role in stem cell differentiation, and a scaffold that mimics native cartilaginous extracellular components can promote chondrogenesis.					
37044324	4	48	theme	mitochondrial	973:985	arg1	survival					1007:1014	high cell survival	997:1014	high cell survival	997:1014	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	4	48	theme	mitochondrial	973:985	arg1	activity					987:994	good mitochondrial activity	968:994	good mitochondrial activity	968:994	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	7	49	from	BMP7	1382:1385	arg1	group					1441:1445	the tetra-copolymer scaffold group	1412:1445	the tetra-copolymer scaffold group	1412:1445	BMP7 and COL2A1 expression in the tetra-copolymer scaffold group was much higher than that in the gelatin scaffold group ex vivo.					
37044324	3	50	theme	biomimetic	654:663	arg1	scaffold					665:672	The newly designed biomimetic scaffold	635:672	The newly designed biomimetic scaffold	635:672	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	6	51	theme	cartilage-like	1294:1307	arg1	tissue					1309:1314	cartilage-like tissue	1294:1314	cartilage-like tissue in SCID mice	1294:1327	Ex vivo study revealed that the SDSC-tetra-copolymer scaffold regenerated cartilage-like tissue in SCID mice with abundant type II collagen and S-100 production.					
37044324	3	52	theme	good	802:805	arg1	resilience					807:816	a good resilience	800:816	a good resilience	800:816	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	1	53	theme	stem	251:254	arg1	differentiation					261:275	stem cell differentiation	251:275	stem cell differentiation	251:275	The microenvironment plays a crucial role in stem cell differentiation, and a scaffold that mimics native cartilaginous extracellular components can promote chondrogenesis.					
37044324	0	54	theme	cells	199:203	arg1	chondrogenesis					153:166	chondrogenesis	153:166	chondrogenesis of human synovium-derived stem cells	153:203	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	3	55	theme	swelling	680:687	arg1	ratio					689:693	a swelling ratio	678:693	a swelling ratio of 1278 % ± 270 %	678:711	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	3	55	theme	swelling	680:687	arg1	strength					761:768	a compressive strength	747:768	a compressive strength of 1005 ± 174 KPa	747:786	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	3	55	theme	swelling	680:687	arg1	porosity					716:723	a porosity	714:723	a porosity of 77.68 % ± 11.70 %	714:744	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	3	56	theme	%	711:711	arg1	ratio					689:693	a swelling ratio	678:693	a swelling ratio of 1278 % ± 270 %	678:711	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	3	56	theme	%	711:711	arg1	strength					761:768	a compressive strength	747:768	a compressive strength of 1005 ± 174 KPa	747:786	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	3	56	theme	%	711:711	arg1	porosity					716:723	a porosity	714:723	a porosity of 77.68 % ± 11.70 %	714:744	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	4	57	theme	pronounced	1023:1032	arg1	glycosaminoglycan					1034:1050	a pronounced glycosaminoglycan	1021:1050	a pronounced glycosaminoglycan production	1021:1061	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	8	58	theme	cartilage	1606:1614	arg1	engineering					1623:1633	cartilage tissue engineering	1606:1633	cartilage tissue engineering	1606:1633	The tetra-copolymer scaffold thus exhibits strong chondrogenic capability and will facilitate cartilage tissue engineering.					
37044324	4	59	theme	cell	1002:1005	arg1	survival					1007:1014	high cell survival	997:1014	high cell survival	997:1014	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	4	59	theme	cell	1002:1005	arg1	activity					987:994	good mitochondrial activity	968:994	good mitochondrial activity	968:994	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	7	60	theme	scaffold	1488:1495	arg1	group					1497:1501	the gelatin scaffold group	1476:1501	the gelatin scaffold group ex vivo	1476:1509	BMP7 and COL2A1 expression in the tetra-copolymer scaffold group was much higher than that in the gelatin scaffold group ex vivo.					
37044324	0	61	theme	extracellular	120:132	arg1	matrix					134:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	2	62	theme	gelatin	617:623	arg1	scaffold					625:632	a pure gelatin scaffold	610:632	a pure gelatin scaffold	610:632	In this study, a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage was fabricated using a microfluidic technique and compared with a pure gelatin scaffold.					
37044324	7	63	theme	gelatin	1480:1486	arg1	group					1497:1501	the gelatin scaffold group	1476:1501	the gelatin scaffold group ex vivo	1476:1509	BMP7 and COL2A1 expression in the tetra-copolymer scaffold group was much higher than that in the gelatin scaffold group ex vivo.					
37044324	4	64	theme	stem	862:865	arg1	cells					867:871	Synovium-derived stem cells	845:871	Synovium-derived stem cells (SDSCs)	845:879	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	4	64	theme	stem	862:865	arg1	SDSCs					874:878	SDSCs	874:878	SDSCs	874:878	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	6	65	from	tissue	1309:1314	arg1	mice					1324:1327	SCID mice	1319:1327	SCID mice	1319:1327	Ex vivo study revealed that the SDSC-tetra-copolymer scaffold regenerated cartilage-like tissue in SCID mice with abundant type II collagen and S-100 production.					
37044324	0	66	theme	synovium-derived	177:192	arg1	cells					199:203	human synovium-derived stem cells	171:203	human synovium-derived stem cells	171:203	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	5	67	theme	BMP-7	1213:1217	arg1	expression					1166:1175	upregulated mRNA expression	1149:1175	upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7	1149:1217	SDSCs cultured on the tetra-copolymer scaffold with chondrogenic induction exhibited upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7.					
37044324	5	68	theme	tetra-copolymer	1086:1100	arg1	scaffold					1102:1109	the tetra-copolymer scaffold	1082:1109	the tetra-copolymer scaffold	1082:1109	SDSCs cultured on the tetra-copolymer scaffold with chondrogenic induction exhibited upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7.					
37044324	0	69	theme	collagen-gelatin-hyaluronic	12:38	arg1	matrix					134:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	0	70	theme	sulfate	57:63	arg1	matrix					134:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	3	71	theme	%	744:744	arg1	porosity					716:723	a porosity	714:723	a porosity of 77.68 % ± 11.70 %	714:744	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	3	71	theme	%	744:744	arg1	ratio					689:693	a swelling ratio	678:693	a swelling ratio of 1278 % ± 270 %	678:711	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	4	72	link	Synovium-derived	845:860	arg1	cells					867:871	Synovium-derived stem cells	845:871	Synovium-derived stem cells (SDSCs)	845:879	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	4	72	link	Synovium-derived	845:860	arg1	SDSCs					874:878	SDSCs	874:878	SDSCs	874:878	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	0	73	theme	scaffold	81:88	arg1	matrix					134:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	5	74	theme	upregulated	1149:1159	arg1	expression					1166:1175	upregulated mRNA expression	1149:1175	upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7	1149:1217	SDSCs cultured on the tetra-copolymer scaffold with chondrogenic induction exhibited upregulated mRNA expression of COL2A1, ChM-1, Nrf2, TGF-β1, and BMP-7.					
37044324	7	75	theme	tetra-copolymer	1416:1430	arg1	group					1441:1445	the tetra-copolymer scaffold group	1412:1445	the tetra-copolymer scaffold group	1412:1445	BMP7 and COL2A1 expression in the tetra-copolymer scaffold group was much higher than that in the gelatin scaffold group ex vivo.					
37044324	4	76	theme	tetra-copolymer	897:911	arg1	scaffold					913:920	the tetra-copolymer scaffold	893:920	the tetra-copolymer scaffold attached to the scaffold firmly	893:952	Synovium-derived stem cells (SDSCs) seeded into the tetra-copolymer scaffold attached to the scaffold firmly and exhibited good mitochondrial activity, high cell survival with a pronounced glycosaminoglycan production.					
37044324	0	77	theme	native	103:108	arg1	matrix					134:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix	0:139	Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells.					
37044324	2	78	theme	sulfate	441:447	arg1	scaffold					465:472	a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold	394:472	a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage	394:544	In this study, a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage was fabricated using a microfluidic technique and compared with a pure gelatin scaffold.					
37044324	2	79	with	scaffold	465:472	arg1	architecture					495:506	architecture	495:506	architecture	495:506	In this study, a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage was fabricated using a microfluidic technique and compared with a pure gelatin scaffold.					
37044324	2	79	with	scaffold	465:472	arg1	composition					479:489	composition	479:489	composition	479:489	In this study, a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage was fabricated using a microfluidic technique and compared with a pure gelatin scaffold.					
37044324	7	80	theme	COL2A1	1391:1396	arg1	expression					1398:1407	COL2A1 expression	1391:1407	COL2A1 expression	1391:1407	BMP7 and COL2A1 expression in the tetra-copolymer scaffold group was much higher than that in the gelatin scaffold group ex vivo.					
37044324	2	81	theme	collagen-gelatin-hyaluronic	396:422	arg1	scaffold					465:472	a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold	394:472	a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage	394:544	In this study, a collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold with composition and architecture similar to those of hyaline cartilage was fabricated using a microfluidic technique and compared with a pure gelatin scaffold.					
37044324	6	82	theme	Ex	1220:1221	arg1	study					1228:1232	Ex vivo study	1220:1232	Ex vivo study	1220:1232	Ex vivo study revealed that the SDSC-tetra-copolymer scaffold regenerated cartilage-like tissue in SCID mice with abundant type II collagen and S-100 production.					
37044324	8	83	theme	chondrogenic	1562:1573	arg1	capability					1575:1584	strong chondrogenic capability	1555:1584	strong chondrogenic capability	1555:1584	The tetra-copolymer scaffold thus exhibits strong chondrogenic capability and will facilitate cartilage tissue engineering.					
37044324	3	84	theme	designed	645:652	arg1	scaffold					665:672	The newly designed biomimetic scaffold	635:672	The newly designed biomimetic scaffold	635:672	The newly designed biomimetic scaffold had a swelling ratio of 1278 % ± 270 %, a porosity of 77.68 % ± 11.70 %, a compressive strength of 1005 ± 174 KPa, and showed a good resilience against compression force.					
37044324	1	85	theme	native	305:310	arg1	components					340:349	native cartilaginous extracellular components	305:349	native cartilaginous extracellular components	305:349	The microenvironment plays a crucial role in stem cell differentiation, and a scaffold that mimics native cartilaginous extracellular components can promote chondrogenesis.					
37024003	4	0	theme	optimization	464:475	arg1	objective					446:454	The basic objective	436:454	The basic objective of this optimization	436:475	The basic objective of this optimization was to maximize fiber yield using hot water as an extraction eco-solvent.					
37024003	9	1	theme	XRD	1245:1247	arg1	pattern					1249:1255	XRD pattern	1245:1255	XRD pattern	1245:1255	FTIR spectrum and XRD pattern were typical to lignocellulosic fibers results.					
37024003	8	2	theme	scale-produced	1204:1217	arg1	fibers					1219:1224	pilot scale-produced fibers	1198:1224	pilot scale-produced fibers	1198:1224	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	12	3	theme	cellulosic	1536:1545	arg1	fibers					1547:1552	cellulosic fibers	1536:1552	cellulosic fibers microstructure	1536:1567	SEM analysis presented elongated and organized cells with a uniform structure comparable to cellulosic fibers microstructure.					
37024003	0	4	theme	dietary	71:77	arg1	fibers					79:84	dietary fibers	71:84	dietary fibers	71:84	Green solid-liquid extraction of cactus (Opuntia ficus-indica) cladode dietary fibers.					
37024003	5	5	theme	extraction	563:572	arg1	time					574:577	The optimum extraction time	551:577	The optimum extraction time (330 min)	551:587	The optimum extraction time (330 min) and temperature (100 °C) were determined with a constant medium agitation rate.					
37024003	5	5	theme	extraction	563:572	arg1	min					584:586	330 min	580:586	330 min	580:586	The optimum extraction time (330 min) and temperature (100 °C) were determined with a constant medium agitation rate.					
37024003	11	6	theme	crystallinity	1423:1435	arg1	index					1437:1441	a 45% crystallinity index	1417:1441	a 45% crystallinity index	1417:1441	Pure and crystallized phases were recorded with a 45% crystallinity index.					
37024003	3	7	theme	composite	333:341	arg1	design					356:361	a central composite experimental design	323:361	a central composite experimental design with two factors (temperature and time) and five levels	323:417	For this purpose, a central composite experimental design with two factors (temperature and time) and five levels was established.					
37024003	10	8	theme	cellulose	1344:1352	arg1	characteristic					1326:1339	characteristic	1326:1339	characteristic	1326:1339	Sharp and thin peaks characteristic of cellulose were detected.					
37024003	6	9	theme	model	755:759	arg1	validation					725:734	the validation	721:734	the validation of the statistical model	721:759	Additionally, this study also aimed at establishing the validation of the statistical model to carry out the extrapolation of the extraction process at the pilot scale.					
37024003	2	10	theme	dietary	275:281	arg1	fibers					283:288	cactus (Opuntia ficus indica) cladode dietary fibers	237:288	cactus (Opuntia ficus indica) cladode dietary fibers	237:288	In this research work, an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers was conducted.					
37024003	3	11	theme	experimental	343:354	arg1	design					356:361	a central composite experimental design	323:361	a central composite experimental design with two factors (temperature and time) and five levels	323:417	For this purpose, a central composite experimental design with two factors (temperature and time) and five levels was established.					
37024003	9	12	theme	FTIR	1227:1230	arg1	spectrum					1232:1239	FTIR spectrum	1227:1239	FTIR spectrum	1227:1239	FTIR spectrum and XRD pattern were typical to lignocellulosic fibers results.					
37024003	3	13	dep	factors	372:378	arg1	temperature					381:391	temperature	381:391	temperature	381:391	For this purpose, a central composite experimental design with two factors (temperature and time) and five levels was established.					
37024003	3	13	dep	factors	372:378	arg1	factors					372:378	two factors	368:378	two factors (temperature and time)	368:401	For this purpose, a central composite experimental design with two factors (temperature and time) and five levels was established.					
37024003	3	13	dep	factors	372:378	arg1	time					397:400	time	397:400	time	397:400	For this purpose, a central composite experimental design with two factors (temperature and time) and five levels was established.					
37024003	6	14	theme	statistical	743:753	arg1	model					755:759	the statistical model	739:759	the statistical model	739:759	Additionally, this study also aimed at establishing the validation of the statistical model to carry out the extrapolation of the extraction process at the pilot scale.					
37024003	2	15	theme	cladode	267:273	arg1	fibers					283:288	cactus (Opuntia ficus indica) cladode dietary fibers	237:288	cactus (Opuntia ficus indica) cladode dietary fibers	237:288	In this research work, an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers was conducted.					
37024003	11	16	theme	Pure	1369:1372	arg1	phases					1391:1396	Pure and crystallized phases	1369:1396	Pure and crystallized phases	1369:1396	Pure and crystallized phases were recorded with a 45% crystallinity index.					
37024003	12	17	theme	elongated	1467:1475	arg1	cells					1491:1495	elongated and organized cells	1467:1495	elongated and organized cells	1467:1495	SEM analysis presented elongated and organized cells with a uniform structure comparable to cellulosic fibers microstructure.					
37024003	4	18	theme	basic	440:444	arg1	objective					446:454	The basic objective	436:454	The basic objective of this optimization	436:475	The basic objective of this optimization was to maximize fiber yield using hot water as an extraction eco-solvent.					
37024003	10	19	theme	Sharp	1305:1309	arg1	peaks					1320:1324	Sharp and thin peaks	1305:1324	Sharp and thin peaks characteristic of cellulose	1305:1352	Sharp and thin peaks characteristic of cellulose were detected.					
37024003	3	20	theme	central	325:331	arg1	design					356:361	a central composite experimental design	323:361	a central composite experimental design with two factors (temperature and time) and five levels	323:417	For this purpose, a central composite experimental design with two factors (temperature and time) and five levels was established.					
37024003	7	21	theme	pilot	866:870	arg1	scale					872:876	the pilot scale	862:876	the pilot scale	862:876	The fibers extracted at the pilot scale showed yields (45.2 ± 0.01%) in agreement with those obtained through the optimization and validation lab-scale steps (44.97 ± 0.02).					
37024003	8	22	theme	Scanning	1088:1095	arg1	microscopy					1106:1115	Scanning electron microscopy	1088:1115	Scanning electron microscopy (SEM) analysis	1088:1130	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	8	22	theme	Scanning	1088:1095	arg1	SEM					1118:1120	SEM	1118:1120	SEM	1118:1120	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	2	23	dep	cactus	237:242	arg1	ficus					253:257	Opuntia ficus	245:257	Opuntia ficus indica	245:264	In this research work, an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers was conducted.					
37024003	0	24	theme	solid-liquid	6:17	arg1	extraction					19:28	Green solid-liquid extraction	0:28	Green solid-liquid extraction of cactus (Opuntia ficus-indica)	0:61	Green solid-liquid extraction of cactus (Opuntia ficus-indica) cladode dietary fibers.					
37024003	8	25	theme	X-ray	1060:1064	arg1	XRD					1079:1081	XRD	1079:1081	XRD	1079:1081	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	8	25	theme	X-ray	1060:1064	arg1	Diffraction					1066:1076	X-ray Diffraction	1060:1076	X-ray Diffraction (XRD)	1060:1082	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	7	26	theme	lab-scale	980:988	arg1	steps					990:994	lab-scale steps	980:994	lab-scale steps	980:994	The fibers extracted at the pilot scale showed yields (45.2 ± 0.01%) in agreement with those obtained through the optimization and validation lab-scale steps (44.97 ± 0.02).					
37024003	3	27	with	design	356:361	arg1	temperature					381:391	temperature	381:391	temperature	381:391	For this purpose, a central composite experimental design with two factors (temperature and time) and five levels was established.					
37024003	3	27	with	design	356:361	arg1	levels					412:417	five levels	407:417	five levels	407:417	For this purpose, a central composite experimental design with two factors (temperature and time) and five levels was established.					
37024003	3	27	with	design	356:361	arg1	factors					372:378	two factors	368:378	two factors (temperature and time)	368:401	For this purpose, a central composite experimental design with two factors (temperature and time) and five levels was established.					
37024003	3	27	with	design	356:361	arg1	time					397:400	time	397:400	time	397:400	For this purpose, a central composite experimental design with two factors (temperature and time) and five levels was established.					
37024003	6	28	theme	pilot	825:829	arg1	scale					831:835	the pilot scale	821:835	the pilot scale	821:835	Additionally, this study also aimed at establishing the validation of the statistical model to carry out the extrapolation of the extraction process at the pilot scale.					
37024003	2	29	dep	ficus	253:257	arg1	indica					259:264	indica	259:264	Opuntia ficus indica	245:264	In this research work, an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers was conducted.					
37024003	0	30	theme	Green	0:4	arg1	extraction					19:28	Green solid-liquid extraction	0:28	Green solid-liquid extraction of cactus (Opuntia ficus-indica)	0:61	Green solid-liquid extraction of cactus (Opuntia ficus-indica) cladode dietary fibers.					
37024003	12	31	theme	fibers	1547:1552	arg1	microstructure					1554:1567	cellulosic fibers microstructure	1536:1567	cellulosic fibers microstructure	1536:1567	SEM analysis presented elongated and organized cells with a uniform structure comparable to cellulosic fibers microstructure.					
37024003	1	32	theme	I-	87:88	arg1	optimization					90:101	I- optimization	87:101	I- optimization	87:101	I- optimization, pilot-scale production, and characterization.					
37024003	8	33	dep	Infrared	1030:1037	arg1	FTIR					1040:1043	FTIR	1040:1043	FTIR	1040:1043	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	5	34	theme	constant	637:644	arg1	rate					663:666	a constant medium agitation rate	635:666	a constant medium agitation rate	635:666	The optimum extraction time (330 min) and temperature (100 °C) were determined with a constant medium agitation rate.					
37024003	0	35	dep	cactus	33:38	arg1	ficus-indica					49:60	Opuntia ficus-indica	41:60	Opuntia ficus-indica	41:60	Green solid-liquid extraction of cactus (Opuntia ficus-indica) cladode dietary fibers.					
37024003	7	36	dep	optimization	952:963	arg1	steps					990:994	lab-scale steps	980:994	lab-scale steps	980:994	The fibers extracted at the pilot scale showed yields (45.2 ± 0.01%) in agreement with those obtained through the optimization and validation lab-scale steps (44.97 ± 0.02).					
37024003	7	36	dep	optimization	952:963	arg1	the					948:950	the	948:950	the	948:950	The fibers extracted at the pilot scale showed yields (45.2 ± 0.01%) in agreement with those obtained through the optimization and validation lab-scale steps (44.97 ± 0.02).					
37024003	8	37	theme	microscopy	1106:1115	arg1	analysis					1123:1130	Scanning electron microscopy (SEM) analysis	1088:1130	Scanning electron microscopy (SEM) analysis	1088:1130	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	4	38	theme	extraction	527:536	arg1	eco-solvent					538:548	an extraction eco-solvent	524:548	an extraction eco-solvent	524:548	The basic objective of this optimization was to maximize fiber yield using hot water as an extraction eco-solvent.					
37024003	4	38	theme	extraction	527:536	arg1	water					515:519	hot water	511:519	hot water	511:519	The basic objective of this optimization was to maximize fiber yield using hot water as an extraction eco-solvent.					
37024003	5	39	theme	medium	646:651	arg1	rate					663:666	a constant medium agitation rate	635:666	a constant medium agitation rate	635:666	The optimum extraction time (330 min) and temperature (100 °C) were determined with a constant medium agitation rate.					
37024003	6	40	theme	process	810:816	arg1	extrapolation					778:790	the extrapolation	774:790	the extrapolation of the extraction process	774:816	Additionally, this study also aimed at establishing the validation of the statistical model to carry out the extrapolation of the extraction process at the pilot scale.					
37024003	2	41	theme	fibers	283:288	arg1	optimization					176:187	an optimization	173:187	an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers	173:288	In this research work, an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers was conducted.					
37024003	11	42	theme	crystallized	1378:1389	arg1	phases					1391:1396	Pure and crystallized phases	1369:1396	Pure and crystallized phases	1369:1396	Pure and crystallized phases were recorded with a 45% crystallinity index.					
37024003	5	43	theme	agitation	653:661	arg1	rate					663:666	a constant medium agitation rate	635:666	a constant medium agitation rate	635:666	The optimum extraction time (330 min) and temperature (100 °C) were determined with a constant medium agitation rate.					
37024003	6	44	theme	extraction	799:808	arg1	process					810:816	the extraction process	795:816	the extraction process	795:816	Additionally, this study also aimed at establishing the validation of the statistical model to carry out the extrapolation of the extraction process at the pilot scale.					
37024003	9	45	theme	typical	1262:1268	arg1	results					1296:1302	typical to lignocellulosic fibers results	1262:1302	typical to lignocellulosic fibers results	1262:1302	FTIR spectrum and XRD pattern were typical to lignocellulosic fibers results.					
37024003	0	46	theme	cactus	33:38	arg1	extraction					19:28	Green solid-liquid extraction	0:28	Green solid-liquid extraction of cactus (Opuntia ficus-indica)	0:61	Green solid-liquid extraction of cactus (Opuntia ficus-indica) cladode dietary fibers.					
37024003	9	47	theme	lignocellulosic	1273:1287	arg1	fibers					1289:1294	lignocellulosic fibers	1273:1294	lignocellulosic fibers	1273:1294	FTIR spectrum and XRD pattern were typical to lignocellulosic fibers results.					
37024003	8	48	dep	Fourier	1012:1018	arg1	Transform					1020:1028	Transform	1020:1028	Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis	1020:1130	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	12	49	theme	SEM	1444:1446	arg1	analysis					1448:1455	SEM analysis	1444:1455	SEM analysis	1444:1455	SEM analysis presented elongated and organized cells with a uniform structure comparable to cellulosic fibers microstructure.					
37024003	2	50	theme	method	227:232	arg1	optimization					176:187	an optimization	173:187	an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers	173:288	In this research work, an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers was conducted.					
37024003	12	51	theme	comparable	1522:1531	arg1	structure					1512:1520	a uniform structure	1502:1520	a uniform structure comparable to cellulosic fibers microstructure	1502:1567	SEM analysis presented elongated and organized cells with a uniform structure comparable to cellulosic fibers microstructure.					
37024003	4	52	theme	fiber	493:497	arg1	yield					499:503	fiber yield	493:503	fiber yield	493:503	The basic objective of this optimization was to maximize fiber yield using hot water as an extraction eco-solvent.					
37024003	2	53	theme	research	158:165	arg1	work					167:170	this research work	153:170	this research work	153:170	In this research work, an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers was conducted.					
37024003	2	54	theme	extraction	216:225	arg1	method					227:232	an environment friendly extraction method	192:232	an environment friendly extraction method	192:232	In this research work, an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers was conducted.					
37024003	1	55	theme	pilot-scale	104:114	arg1	production					116:125	pilot-scale production	104:125	pilot-scale production	104:125	I- optimization, pilot-scale production, and characterization.					
37024003	4	56	theme	hot	511:513	arg1	eco-solvent					538:548	an extraction eco-solvent	524:548	an extraction eco-solvent	524:548	The basic objective of this optimization was to maximize fiber yield using hot water as an extraction eco-solvent.					
37024003	4	56	theme	hot	511:513	arg1	water					515:519	hot water	511:519	hot water	511:519	The basic objective of this optimization was to maximize fiber yield using hot water as an extraction eco-solvent.					
37024003	8	57	theme	Infrared	1030:1037	arg1	spectroscopy					1046:1057	Infrared (FTIR) spectroscopy	1030:1057	Infrared (FTIR) spectroscopy	1030:1057	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	2	58	theme	environment	195:205	arg1	method					227:232	an environment friendly extraction method	192:232	an environment friendly extraction method	192:232	In this research work, an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers was conducted.					
37024003	2	59	theme	friendly	207:214	arg1	method					227:232	an environment friendly extraction method	192:232	an environment friendly extraction method	192:232	In this research work, an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers was conducted.					
37024003	8	60	theme	pilot	1198:1202	arg1	fibers					1219:1224	pilot scale-produced fibers	1198:1224	pilot scale-produced fibers	1198:1224	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	11	61	theme	45	1419:1420	arg1	%					1421:1421	%	1421:1421	%	1421:1421	Pure and crystallized phases were recorded with a 45% crystallinity index.					
37024003	5	62	theme	optimum	555:561	arg1	time					574:577	The optimum extraction time	551:577	The optimum extraction time (330 min)	551:587	The optimum extraction time (330 min) and temperature (100 °C) were determined with a constant medium agitation rate.					
37024003	5	62	theme	optimum	555:561	arg1	min					584:586	330 min	580:586	330 min	580:586	The optimum extraction time (330 min) and temperature (100 °C) were determined with a constant medium agitation rate.					
37024003	10	63	theme	thin	1315:1318	arg1	peaks					1320:1324	Sharp and thin peaks	1305:1324	Sharp and thin peaks characteristic of cellulose	1305:1352	Sharp and thin peaks characteristic of cellulose were detected.					
37024003	11	64	theme	%	1421:1421	arg1	index					1437:1441	a 45% crystallinity index	1417:1441	a 45% crystallinity index	1417:1441	Pure and crystallized phases were recorded with a 45% crystallinity index.					
37024003	8	65	dep	structure	1166:1174	arg1	the					1162:1164	the	1162:1164	the	1162:1164	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	12	66	theme	uniform	1504:1510	arg1	structure					1512:1520	a uniform structure	1502:1520	a uniform structure comparable to cellulosic fibers microstructure	1502:1567	SEM analysis presented elongated and organized cells with a uniform structure comparable to cellulosic fibers microstructure.					
37024003	8	67	theme	fibers	1219:1224	arg1	microstructure					1180:1193	microstructure	1180:1193	microstructure	1180:1193	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	8	67	theme	fibers	1219:1224	arg1	structure					1166:1174	structure	1166:1174	structure	1166:1174	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	12	68	theme	organized	1481:1489	arg1	cells					1491:1495	elongated and organized cells	1467:1495	elongated and organized cells	1467:1495	SEM analysis presented elongated and organized cells with a uniform structure comparable to cellulosic fibers microstructure.					
37024003	10	69	theme	characteristic	1326:1339	arg1	peaks					1320:1324	Sharp and thin peaks	1305:1324	Sharp and thin peaks characteristic of cellulose	1305:1352	Sharp and thin peaks characteristic of cellulose were detected.					
37024003	2	70	theme	cactus	237:242	arg1	fibers					283:288	cactus (Opuntia ficus indica) cladode dietary fibers	237:288	cactus (Opuntia ficus indica) cladode dietary fibers	237:288	In this research work, an optimization of an environment friendly extraction method of cactus (Opuntia ficus indica) cladode dietary fibers was conducted.					
37024003	8	71	theme	electron	1097:1104	arg1	microscopy					1106:1115	Scanning electron microscopy	1088:1115	Scanning electron microscopy (SEM) analysis	1088:1130	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	8	71	theme	electron	1097:1104	arg1	SEM					1118:1120	SEM	1118:1120	SEM	1118:1120	Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and Scanning electron microscopy (SEM) analysis were conducted to investigate the structure and microstructure of pilot scale-produced fibers.					
37024003	7	72	with	agreement	910:918	arg1	those					925:929	those	925:929	those	925:929	The fibers extracted at the pilot scale showed yields (45.2 ± 0.01%) in agreement with those obtained through the optimization and validation lab-scale steps (44.97 ± 0.02).					
36372507	5	0	theme	size	743:746	arg1	distribution					748:759	the size distribution	739:759	the size distribution of the magnetic nanoparticles (MNPs) between 39.9 and 73.3 nm	739:821	The FE-SEM images were taken to evaluate the size distribution of the magnetic nanoparticles (MNPs) between 39.9 and 73.3 nm as well.					
36372507	4	1	theme	VSM	684:686	arg1	analysis					688:695	VSM analysis	684:695	VSM analysis	684:695	Various characteristics of this novel magnetic nanobiocomposite were distinguished by FT-IR, EDX, FE-SEM, XRD, TGA, and VSM analysis.					
36372507	1	2	theme	graphene	255:262	arg1	oxide					264:268	graphene oxide	255:268	graphene oxide (GO)	255:273	This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs.					
36372507	2	3	theme	cross-linking	331:343	arg1	agent					345:349	a cross-linking agent	329:349	a cross-linking agent to cross-link the CS strings	329:378	Terephthaloyl thiourea was applied as a cross-linking agent to cross-link the CS strings.					
36372507	2	3	theme	cross-linking	331:343	arg1	thiourea					305:312	Terephthaloyl thiourea	291:312	Terephthaloyl thiourea	291:312	Terephthaloyl thiourea was applied as a cross-linking agent to cross-link the CS strings.					
36372507	1	4	theme	oxide	264:268	arg1	NPs					286:288	chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	212:288	chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	212:288	This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs.					
36372507	3	5	from	biocompatibility	508:523	arg1	solution					554:561	an aqueous solution	543:561	an aqueous solution	543:561	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	4	6	theme	magnetic	602:609	arg1	nanobiocomposite					611:626	this novel magnetic nanobiocomposite	591:626	this novel magnetic nanobiocomposite	591:626	Various characteristics of this novel magnetic nanobiocomposite were distinguished by FT-IR, EDX, FE-SEM, XRD, TGA, and VSM analysis.					
36372507	6	7	theme	nanobiocomposite	864:879	arg1	performance					836:846	The performance	832:846	The performance of the prepared nanobiocomposite	832:879	The performance of the prepared nanobiocomposite was assessed by the magnetic fluid hyperthermia process.					
36372507	3	8	theme	aqueous	546:552	arg1	solution					554:561	an aqueous solution	543:561	an aqueous solution	543:561	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	4	9	theme	novel	596:600	arg1	nanobiocomposite					611:626	this novel magnetic nanobiocomposite	591:626	this novel magnetic nanobiocomposite	591:626	Various characteristics of this novel magnetic nanobiocomposite were distinguished by FT-IR, EDX, FE-SEM, XRD, TGA, and VSM analysis.					
36372507	3	10	theme	thermal	489:495	arg1	stability					497:505	thermal stability	489:505	thermal stability	489:505	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	1	11	theme	chitosan	212:219	arg1	NPs					286:288	chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	212:288	chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	212:288	This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs.					
36372507	5	12	theme	nanoparticles	777:789	arg1	distribution					748:759	the size distribution	739:759	the size distribution of the magnetic nanoparticles (MNPs) between 39.9 and 73.3 nm	739:821	The FE-SEM images were taken to evaluate the size distribution of the magnetic nanoparticles (MNPs) between 39.9 and 73.3 nm as well.					
36372507	3	13	from	uniformity	477:486	arg1	solution					554:561	an aqueous solution	543:561	an aqueous solution	543:561	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	0	14	theme	chitosan-silk	9:21	arg1	oxide					49:53	Magnetic chitosan-silk fibroin hydrogel/graphene oxide	0:53	Magnetic chitosan-silk fibroin hydrogel/graphene oxide	0:53	Magnetic chitosan-silk fibroin hydrogel/graphene oxide nanobiocomposite for biological and hyperthermia applications.					
36372507	4	15	theme	Various	564:570	arg1	characteristics					572:586	Various characteristics	564:586	Various characteristics of this novel magnetic nanobiocomposite	564:626	Various characteristics of this novel magnetic nanobiocomposite were distinguished by FT-IR, EDX, FE-SEM, XRD, TGA, and VSM analysis.					
36372507	6	16	theme	prepared	855:862	arg1	nanobiocomposite					864:879	the prepared nanobiocomposite	851:879	the prepared nanobiocomposite	851:879	The performance of the prepared nanobiocomposite was assessed by the magnetic fluid hyperthermia process.					
36372507	2	17	theme	CS	369:370	arg1	strings					372:378	the CS strings	365:378	the CS strings	365:378	Terephthaloyl thiourea was applied as a cross-linking agent to cross-link the CS strings.					
36372507	0	18	theme	Magnetic	0:7	arg1	oxide					49:53	Magnetic chitosan-silk fibroin hydrogel/graphene oxide	0:53	Magnetic chitosan-silk fibroin hydrogel/graphene oxide	0:53	Magnetic chitosan-silk fibroin hydrogel/graphene oxide nanobiocomposite for biological and hyperthermia applications.					
36372507	7	19	theme	rate	1024:1027	arg1	value					991:995	the mean value	982:995	the mean value of the specific absorption rate (SAR)	982:1033	Under the alternating magnetic field (AMF), the mean value of the specific absorption rate (SAR) was determined at 43.15 w/g.					
36372507	2	20	theme	Terephthaloyl	291:303	arg1	agent					345:349	a cross-linking agent	329:349	a cross-linking agent to cross-link the CS strings	329:378	Terephthaloyl thiourea was applied as a cross-linking agent to cross-link the CS strings.					
36372507	2	20	theme	Terephthaloyl	291:303	arg1	thiourea					305:312	Terephthaloyl thiourea	291:312	Terephthaloyl thiourea	291:312	Terephthaloyl thiourea was applied as a cross-linking agent to cross-link the CS strings.					
36372507	3	21	theme	many	431:434	arg1	characteristics					436:450	many characteristics	431:450	many characteristics	431:450	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	3	21	theme	many	431:434	arg1	biocompatibility					508:523	biocompatibility	508:523	biocompatibility	508:523	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	3	21	theme	many	431:434	arg1	stability					530:538	stability	530:538	stability	530:538	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	3	21	theme	many	431:434	arg1	stability					497:505	thermal stability	489:505	thermal stability	489:505	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	3	21	theme	many	431:434	arg1	uniformity					477:486	high structural uniformity	461:486	high structural uniformity	461:486	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	0	22	theme	hydrogel/graphene	31:47	arg1	oxide					49:53	Magnetic chitosan-silk fibroin hydrogel/graphene oxide	0:53	Magnetic chitosan-silk fibroin hydrogel/graphene oxide	0:53	Magnetic chitosan-silk fibroin hydrogel/graphene oxide nanobiocomposite for biological and hyperthermia applications.					
36372507	3	23	from	stability	497:505	arg1	solution					554:561	an aqueous solution	543:561	an aqueous solution	543:561	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	3	24	theme	high	461:464	arg1	uniformity					477:486	high structural uniformity	461:486	high structural uniformity	461:486	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	0	25	theme	fibroin	23:29	arg1	oxide					49:53	Magnetic chitosan-silk fibroin hydrogel/graphene oxide	0:53	Magnetic chitosan-silk fibroin hydrogel/graphene oxide	0:53	Magnetic chitosan-silk fibroin hydrogel/graphene oxide nanobiocomposite for biological and hyperthermia applications.					
36372507	3	26	theme	structural	466:475	arg1	uniformity					477:486	high structural uniformity	461:486	high structural uniformity	461:486	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	1	27	theme	Fe3O4	280:284	arg1	NPs					286:288	chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	212:288	chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	212:288	This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs.					
36372507	7	28	theme	absorption	1013:1022	arg1	rate					1024:1027	the specific absorption rate	1000:1027	the specific absorption rate (SAR)	1000:1033	Under the alternating magnetic field (AMF), the mean value of the specific absorption rate (SAR) was determined at 43.15 w/g.					
36372507	1	29	theme	silk	236:239	arg1	SF					250:251	SF	250:251	SF	250:251	This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs.					
36372507	1	29	theme	silk	236:239	arg1	fibroin					241:247	silk fibroin	236:247	silk fibroin (SF)	236:252	This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs.					
36372507	1	30	theme	NPs	286:288	arg1	composition					197:207	the composition	193:207	the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	193:288	This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs.					
36372507	5	31	theme	FE-SEM	702:707	arg1	images					709:714	The FE-SEM images	698:714	The FE-SEM images	698:714	The FE-SEM images were taken to evaluate the size distribution of the magnetic nanoparticles (MNPs) between 39.9 and 73.3 nm as well.					
36372507	1	32	theme	fibroin	241:247	arg1	NPs					286:288	chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	212:288	chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	212:288	This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs.					
36372507	0	33	theme	biological	76:85	arg1	applications					104:115	biological and hyperthermia applications	76:115	biological and hyperthermia applications	76:115	Magnetic chitosan-silk fibroin hydrogel/graphene oxide nanobiocomposite for biological and hyperthermia applications.					
36372507	6	34	theme	hyperthermia	916:927	arg1	process					929:935	the magnetic fluid hyperthermia process	897:935	the magnetic fluid hyperthermia process	897:935	The performance of the prepared nanobiocomposite was assessed by the magnetic fluid hyperthermia process.					
36372507	7	35	dep	rate	1024:1027	arg1	SAR					1030:1032	SAR	1030:1032	SAR	1030:1032	Under the alternating magnetic field (AMF), the mean value of the specific absorption rate (SAR) was determined at 43.15 w/g.					
36372507	7	36	theme	mean	986:989	arg1	value					991:995	the mean value	982:995	the mean value of the specific absorption rate (SAR)	982:1033	Under the alternating magnetic field (AMF), the mean value of the specific absorption rate (SAR) was determined at 43.15 w/g.					
36372507	1	37	theme	biocompatible	141:153	arg1	nanobiocomposite					164:179	a biocompatible magnetic nanobiocomposite	139:179	a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	139:288	This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs.					
36372507	3	38	theme	CS	385:386	arg1	hydrogel/SF/GO/Fe3O4					388:407	The CS hydrogel/SF/GO/Fe3O4	381:407	The CS hydrogel/SF/GO/Fe3O4	381:407	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36372507	6	39	theme	fluid	910:914	arg1	process					929:935	the magnetic fluid hyperthermia process	897:935	the magnetic fluid hyperthermia process	897:935	The performance of the prepared nanobiocomposite was assessed by the magnetic fluid hyperthermia process.					
36372507	7	40	theme	specific	1004:1011	arg1	rate					1024:1027	the specific absorption rate	1000:1027	the specific absorption rate (SAR)	1000:1033	Under the alternating magnetic field (AMF), the mean value of the specific absorption rate (SAR) was determined at 43.15 w/g.					
36372507	1	41	theme	magnetic	155:162	arg1	nanobiocomposite					164:179	a biocompatible magnetic nanobiocomposite	139:179	a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	139:288	This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs.					
36372507	6	42	theme	magnetic	901:908	arg1	process					929:935	the magnetic fluid hyperthermia process	897:935	the magnetic fluid hyperthermia process	897:935	The performance of the prepared nanobiocomposite was assessed by the magnetic fluid hyperthermia process.					
36372507	7	43	theme	alternating	948:958	arg1	AMF					976:978	AMF	976:978	AMF	976:978	Under the alternating magnetic field (AMF), the mean value of the specific absorption rate (SAR) was determined at 43.15 w/g.					
36372507	7	43	theme	alternating	948:958	arg1	field					969:973	the alternating magnetic field	944:973	the alternating magnetic field (AMF)	944:979	Under the alternating magnetic field (AMF), the mean value of the specific absorption rate (SAR) was determined at 43.15 w/g.					
36372507	4	44	theme	nanobiocomposite	611:626	arg1	characteristics					572:586	Various characteristics	564:586	Various characteristics of this novel magnetic nanobiocomposite	564:626	Various characteristics of this novel magnetic nanobiocomposite were distinguished by FT-IR, EDX, FE-SEM, XRD, TGA, and VSM analysis.					
36372507	0	45	theme	hyperthermia	91:102	arg1	applications					104:115	biological and hyperthermia applications	76:115	biological and hyperthermia applications	76:115	Magnetic chitosan-silk fibroin hydrogel/graphene oxide nanobiocomposite for biological and hyperthermia applications.					
36372507	1	46	dep	oxide	264:268	arg1	GO					271:272	GO	271:272	GO	271:272	This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs.					
36372507	1	47	theme	hydrogel	226:233	arg1	NPs					286:288	chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	212:288	chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs	212:288	This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs.					
36372507	5	48	theme	magnetic	768:775	arg1	MNPs					792:795	MNPs	792:795	MNPs	792:795	The FE-SEM images were taken to evaluate the size distribution of the magnetic nanoparticles (MNPs) between 39.9 and 73.3 nm as well.					
36372507	5	48	theme	magnetic	768:775	arg1	nanoparticles					777:789	the magnetic nanoparticles	764:789	the magnetic nanoparticles (MNPs)	764:796	The FE-SEM images were taken to evaluate the size distribution of the magnetic nanoparticles (MNPs) between 39.9 and 73.3 nm as well.					
36372507	7	49	theme	magnetic	960:967	arg1	AMF					976:978	AMF	976:978	AMF	976:978	Under the alternating magnetic field (AMF), the mean value of the specific absorption rate (SAR) was determined at 43.15 w/g.					
36372507	7	49	theme	magnetic	960:967	arg1	field					969:973	the alternating magnetic field	944:973	the alternating magnetic field (AMF)	944:979	Under the alternating magnetic field (AMF), the mean value of the specific absorption rate (SAR) was determined at 43.15 w/g.					
36372507	3	50	from	stability	530:538	arg1	solution					554:561	an aqueous solution	543:561	an aqueous solution	543:561	The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution.					
36318369	7	0	dep	CONCLUSION	1069:1078	arg1	provides					1091:1098	provides	1091:1098	provides a novel and effective approach to alleviate goaty flavor and promote the competitiveness of goat milk products	1091:1209	CONCLUSION This study provides a novel and effective approach to alleviate goaty flavor and promote the competitiveness of goat milk products.					
36318369	6	1	theme	C10	817:819	arg1	acid					811:814	decanoic acid	802:814	decanoic acid (C10 H20 O2 )	802:828	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	6	1	theme	C10	817:819	arg1	H20					821:823	C10 H20 O2	817:826	C10 H20 O2	817:826	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	6	2	theme	C8	786:787	arg1	acid					780:783	octanoic acid	771:783	octanoic acid (C8 H6 O2 )	771:795	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	6	2	theme	C8	786:787	arg1	H6					789:790	C8 H6 O2	786:793	C8 H6 O2	786:793	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	8	3	theme	Chemical	1230:1237	arg1	Industry					1239:1246	Chemical Industry	1230:1246	Chemical Industry	1230:1246	© 2022 Society of Chemical Industry.					
36318369	3	4	theme	goaty	338:342	arg1	flavor					344:349	the goaty flavor	334:349	the goaty flavor	334:349	It is imperative to alleviate the goaty flavor in a safe and efficient way.					
36318369	5	5	theme	β-cyclodextrin	607:620	arg1	supplementation					588:602	the supplementation	584:602	the supplementation of β-cyclodextrin and lactitol	584:633	Furthermore, the supplementation of β-cyclodextrin and lactitol had a synergistic effect in reducing the content of free fatty acids that cause goaty flavor.					
36318369	7	6	theme	effective	1112:1120	arg1	approach					1122:1129	a novel and effective approach	1100:1129	a novel and effective approach to alleviate goaty flavor and promote the competitiveness of goat milk products	1100:1209	CONCLUSION This study provides a novel and effective approach to alleviate goaty flavor and promote the competitiveness of goat milk products.					
36318369	6	7	theme	acid	780:783	arg1	content					733:739	The content	729:739	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 )	729:828	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	5	8	contain	had	635:637	arg1	supplementation					588:602	the supplementation	584:602	the supplementation of β-cyclodextrin and lactitol	584:633	Furthermore, the supplementation of β-cyclodextrin and lactitol had a synergistic effect in reducing the content of free fatty acids that cause goaty flavor.					
36318369	5	8	contain	had	635:637	arg2	effect					653:658	a synergistic effect	639:658	a synergistic effect	639:658	Furthermore, the supplementation of β-cyclodextrin and lactitol had a synergistic effect in reducing the content of free fatty acids that cause goaty flavor.					
36318369	4	9	theme	β-cyclodextrin	446:459	arg1	supplementation					418:432	the supplementation	414:432	the supplementation of 6 g kg-1 β-cyclodextrin or 8 g kg-1 lactitol in goat milk	414:493	RESULTS This study indicated that the supplementation of 6 g kg-1 β-cyclodextrin or 8 g kg-1 lactitol in goat milk significantly alleviated goaty flavor and improved sensory characteristics.					
36318369	5	10	theme	lactitol	626:633	arg1	supplementation					588:602	the supplementation	584:602	the supplementation of β-cyclodextrin and lactitol	584:633	Furthermore, the supplementation of β-cyclodextrin and lactitol had a synergistic effect in reducing the content of free fatty acids that cause goaty flavor.					
36318369	1	11	theme	BACKGROUND	69:78	arg1	milk					85:88	BACKGROUND Goat milk	69:88	BACKGROUND Goat milk	69:88	BACKGROUND Goat milk has balanced nutritional composition, is conducive to digestion and absorption, and does not easily lead to allergic reactions.					
36318369	7	12	theme	novel	1102:1106	arg1	approach					1122:1129	a novel and effective approach	1100:1129	a novel and effective approach to alleviate goaty flavor and promote the competitiveness of goat milk products	1100:1209	CONCLUSION This study provides a novel and effective approach to alleviate goaty flavor and promote the competitiveness of goat milk products.					
36318369	4	13	theme	lactitol	473:480	arg1	supplementation					418:432	the supplementation	414:432	the supplementation of 6 g kg-1 β-cyclodextrin or 8 g kg-1 lactitol in goat milk	414:493	RESULTS This study indicated that the supplementation of 6 g kg-1 β-cyclodextrin or 8 g kg-1 lactitol in goat milk significantly alleviated goaty flavor and improved sensory characteristics.					
36318369	1	14	theme	Goat	80:83	arg1	milk					85:88	BACKGROUND Goat milk	69:88	BACKGROUND Goat milk	69:88	BACKGROUND Goat milk has balanced nutritional composition, is conducive to digestion and absorption, and does not easily lead to allergic reactions.					
36318369	3	15	theme	safe	356:359	arg1	way					375:377	a safe and efficient way	354:377	a safe and efficient way	354:377	It is imperative to alleviate the goaty flavor in a safe and efficient way.					
36318369	4	16	theme	8 g kg-1	464:471	arg1	lactitol					473:480	8 g kg-1 lactitol	464:480	8 g kg-1 lactitol	464:480	RESULTS This study indicated that the supplementation of 6 g kg-1 β-cyclodextrin or 8 g kg-1 lactitol in goat milk significantly alleviated goaty flavor and improved sensory characteristics.					
36318369	5	17	theme	synergistic	641:651	arg1	effect					653:658	a synergistic effect	639:658	a synergistic effect	639:658	Furthermore, the supplementation of β-cyclodextrin and lactitol had a synergistic effect in reducing the content of free fatty acids that cause goaty flavor.					
36318369	5	18	theme	goaty	715:719	arg1	flavor					721:726	goaty flavor	715:726	goaty flavor	715:726	Furthermore, the supplementation of β-cyclodextrin and lactitol had a synergistic effect in reducing the content of free fatty acids that cause goaty flavor.					
36318369	4	19	theme	sensory	546:552	arg1	characteristics					554:568	sensory characteristics	546:568	sensory characteristics	546:568	RESULTS This study indicated that the supplementation of 6 g kg-1 β-cyclodextrin or 8 g kg-1 lactitol in goat milk significantly alleviated goaty flavor and improved sensory characteristics.					
36318369	7	20	theme	products	1202:1209	arg1	competitiveness					1173:1187	the competitiveness	1169:1187	the competitiveness of goat milk products	1169:1209	CONCLUSION This study provides a novel and effective approach to alleviate goaty flavor and promote the competitiveness of goat milk products.					
36318369	6	21	theme	7 g kg-1	958:965	arg1	lactitol					967:974	7 g kg-1 lactitol	958:974	7 g kg-1 lactitol	958:974	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	6	22	theme	octanoic	771:778	arg1	acid					780:783	octanoic acid	771:783	octanoic acid (C8 H6 O2 )	771:795	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	6	22	theme	octanoic	771:778	arg1	H6					789:790	C8 H6 O2	786:793	C8 H6 O2	786:793	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	1	23	theme	balanced	94:101	arg1	composition					115:125	balanced nutritional composition	94:125	balanced nutritional composition	94:125	BACKGROUND Goat milk has balanced nutritional composition, is conducive to digestion and absorption, and does not easily lead to allergic reactions.					
36318369	1	24	theme	nutritional	103:113	arg1	composition					115:125	balanced nutritional composition	94:125	balanced nutritional composition	94:125	BACKGROUND Goat milk has balanced nutritional composition, is conducive to digestion and absorption, and does not easily lead to allergic reactions.					
36318369	2	25	from	flavor	245:250	arg1	milk					255:258	milk	255:258	milk	255:258	However, the special goaty flavor in milk has seriously affected consumer acceptance.					
36318369	7	26	theme	milk	1197:1200	arg1	products					1202:1209	goat milk products	1192:1209	goat milk products	1192:1209	CONCLUSION This study provides a novel and effective approach to alleviate goaty flavor and promote the competitiveness of goat milk products.					
36318369	4	27	from	supplementation	418:432	arg1	milk					490:493	goat milk	485:493	goat milk	485:493	RESULTS This study indicated that the supplementation of 6 g kg-1 β-cyclodextrin or 8 g kg-1 lactitol in goat milk significantly alleviated goaty flavor and improved sensory characteristics.					
36318369	6	28	theme	acid	811:814	arg1	content					733:739	The content	729:739	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 )	729:828	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	6	29	theme	6 g kg-1	930:937	arg1	β-cyclodextrin					939:952	6 g kg-1 β-cyclodextrin	930:952	6 g kg-1 β-cyclodextrin	930:952	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	4	30	theme	goaty	520:524	arg1	flavor					526:531	goaty flavor	520:531	goaty flavor	520:531	RESULTS This study indicated that the supplementation of 6 g kg-1 β-cyclodextrin or 8 g kg-1 lactitol in goat milk significantly alleviated goaty flavor and improved sensory characteristics.					
36318369	4	31	theme	6 g kg-1	437:444	arg1	β-cyclodextrin					446:459	6 g kg-1 β-cyclodextrin	437:459	6 g kg-1 β-cyclodextrin	437:459	RESULTS This study indicated that the supplementation of 6 g kg-1 β-cyclodextrin or 8 g kg-1 lactitol in goat milk significantly alleviated goaty flavor and improved sensory characteristics.					
36318369	6	32	theme	C6	758:759	arg1	acid					752:755	caproic acid	744:755	caproic acid (C6 H12 O2 )	744:768	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	6	32	theme	C6	758:759	arg1	H12					761:763	C6 H12 O2	758:766	C6 H12 O2	758:766	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	1	33	theme	allergic	198:205	arg1	reactions					207:215	allergic reactions	198:215	allergic reactions	198:215	BACKGROUND Goat milk has balanced nutritional composition, is conducive to digestion and absorption, and does not easily lead to allergic reactions.					
36318369	3	34	theme	efficient	365:373	arg1	way					375:377	a safe and efficient way	354:377	a safe and efficient way	354:377	It is imperative to alleviate the goaty flavor in a safe and efficient way.					
36318369	0	35	theme	flavor	61:66	arg1	intensity					42:50	the intensity	38:50	the intensity of goaty flavor	38:66	Lactitol and β-cyclodextrin alleviate the intensity of goaty flavor.					
36318369	7	36	theme	goat	1192:1195	arg1	products					1202:1209	goat milk products	1192:1209	goat milk products	1192:1209	CONCLUSION This study provides a novel and effective approach to alleviate goaty flavor and promote the competitiveness of goat milk products.					
36318369	1	37	contain	has	90:92	arg2	composition					115:125	balanced nutritional composition	94:125	balanced nutritional composition	94:125	BACKGROUND Goat milk has balanced nutritional composition, is conducive to digestion and absorption, and does not easily lead to allergic reactions.					
36318369	1	37	contain	has	90:92	arg1	milk					85:88	BACKGROUND Goat milk	69:88	BACKGROUND Goat milk	69:88	BACKGROUND Goat milk has balanced nutritional composition, is conducive to digestion and absorption, and does not easily lead to allergic reactions.					
36318369	5	38	theme	free	687:690	arg1	acids					698:702	free fatty acids	687:702	free fatty acids that cause goaty flavor	687:726	Furthermore, the supplementation of β-cyclodextrin and lactitol had a synergistic effect in reducing the content of free fatty acids that cause goaty flavor.					
36318369	6	39	theme	acid	752:755	arg1	content					733:739	The content	729:739	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 )	729:828	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	6	40	theme	decanoic	802:809	arg1	acid					811:814	decanoic acid	802:814	decanoic acid (C10 H20 O2 )	802:828	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	6	40	theme	decanoic	802:809	arg1	H20					821:823	C10 H20 O2	817:826	C10 H20 O2	817:826	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	5	41	theme	fatty	692:696	arg1	acids					698:702	free fatty acids	687:702	free fatty acids that cause goaty flavor	687:726	Furthermore, the supplementation of β-cyclodextrin and lactitol had a synergistic effect in reducing the content of free fatty acids that cause goaty flavor.					
36318369	6	42	theme	caproic	744:750	arg1	acid					752:755	caproic acid	744:755	caproic acid (C6 H12 O2 )	744:768	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	6	42	theme	caproic	744:750	arg1	H12					761:763	C6 H12 O2	758:766	C6 H12 O2	758:766	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
36318369	2	43	theme	consumer	283:290	arg1	acceptance					292:301	consumer acceptance	283:301	consumer acceptance	283:301	However, the special goaty flavor in milk has seriously affected consumer acceptance.					
36318369	5	44	theme	acids	698:702	arg1	content					676:682	the content	672:682	the content of free fatty acids that cause goaty flavor	672:726	Furthermore, the supplementation of β-cyclodextrin and lactitol had a synergistic effect in reducing the content of free fatty acids that cause goaty flavor.					
36318369	2	45	theme	goaty	239:243	arg1	flavor					245:250	the special goaty flavor	227:250	the special goaty flavor in milk	227:258	However, the special goaty flavor in milk has seriously affected consumer acceptance.					
36318369	2	46	theme	special	231:237	arg1	flavor					245:250	the special goaty flavor	227:250	the special goaty flavor in milk	227:258	However, the special goaty flavor in milk has seriously affected consumer acceptance.					
36318369	7	47	theme	goaty	1144:1148	arg1	flavor					1150:1155	goaty flavor	1144:1155	goaty flavor	1144:1155	CONCLUSION This study provides a novel and effective approach to alleviate goaty flavor and promote the competitiveness of goat milk products.					
36318369	0	48	theme	goaty	55:59	arg1	flavor					61:66	goaty flavor	55:66	goaty flavor	55:66	Lactitol and β-cyclodextrin alleviate the intensity of goaty flavor.					
36318369	4	49	theme	goat	485:488	arg1	milk					490:493	goat milk	485:493	goat milk	485:493	RESULTS This study indicated that the supplementation of 6 g kg-1 β-cyclodextrin or 8 g kg-1 lactitol in goat milk significantly alleviated goaty flavor and improved sensory characteristics.					
36318369	4	50	dep	RESULTS	380:386	arg1	indicated					399:407	indicated	399:407	indicated that the supplementation of 6 g kg-1 β-cyclodextrin or 8 g kg-1 lactitol in goat milk significantly alleviated goaty flavor and improved sensory characteristics	399:568	RESULTS This study indicated that the supplementation of 6 g kg-1 β-cyclodextrin or 8 g kg-1 lactitol in goat milk significantly alleviated goaty flavor and improved sensory characteristics.					
36318369	6	51	theme	combined	893:900	arg1	group					902:906	a combined group	891:906	a combined group	891:906	The content of caproic acid (C6 H12 O2 ), octanoic acid (C8 H6 O2 ), and decanoic acid (C10 H20 O2 ) decreased by 42.46%, 39.45%, and 46.41%, respectively, after a combined group was supplemented with 6 g kg-1 β-cyclodextrin and 7 g kg-1 lactitol, which was significantly lower than in groups given β-cyclodextrin or lactitol individually.					
35678531	3	0	with	treatment	783:791	arg1	formation					807:815	suitable formation	798:815	suitable formation of plasma-generated RONS	798:840	In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS.					
35678531	6	1	theme	promising	1081:1089	arg1	results					1091:1097	These promising results	1075:1097	These promising results	1075:1097	These promising results postulate plasma-treated thermosensitive hydrogels as good candidates to provide local anticancer therapies.					
35678531	2	2	theme	delivery	355:362	arg1	vehicles					364:371	delivery vehicles	355:371	delivery vehicles that allow a controlled release of RONS to the diseased site	355:432	Acting as delivery vehicles that allow a controlled release of RONS to the diseased site, plasma-treated hydrogels can overcome some of the limitations presented by plasma-treated liquids in in vivo therapies.					
35678531	1	3	theme	gas-plasma	248:257	arg1	treatment					259:267	gas-plasma treatment	248:267	gas-plasma treatment	248:267	Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies.					
35678531	0	4	theme	case	94:97	arg1	study					99:103	a case study	92:103	a case study with methylcellulose	92:124	Thermosensitive hydrogels to deliver reactive species generated by cold atmospheric plasma: a case study with methylcellulose.					
35678531	4	5	theme	RONS	931:934	arg1	release					920:926	release	920:926	release of RONS	920:934	MC hydrogels demonstrated the capacity for generation, prolonged storage and release of RONS.					
35678531	4	5	theme	RONS	931:934	arg1	generation					886:895	generation	886:895	generation	886:895	MC hydrogels demonstrated the capacity for generation, prolonged storage and release of RONS.					
35678531	4	5	theme	RONS	931:934	arg1	storage					908:914	prolonged storage	898:914	prolonged storage	898:914	MC hydrogels demonstrated the capacity for generation, prolonged storage and release of RONS.					
35678531	5	6	theme	cytotoxic	958:966	arg1	effects					968:974	cytotoxic effects	958:974	cytotoxic effects	958:974	This release induced cytotoxic effects on the osteosarcoma cancer cell line MG-63, reducing its cell viability in a dose-response manner.					
35678531	3	7	theme	suitable	798:805	arg1	formation					807:815	suitable formation	798:815	suitable formation of plasma-generated RONS	798:840	In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS.					
35678531	6	8	theme	anticancer	1186:1195	arg1	therapies					1197:1205	local anticancer therapies	1180:1205	local anticancer therapies	1180:1205	These promising results postulate plasma-treated thermosensitive hydrogels as good candidates to provide local anticancer therapies.					
35678531	1	9	theme	suitable	168:175	arg1	Hydrogels					127:135	Hydrogels	127:135	Hydrogels	127:135	Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies.					
35678531	1	9	theme	suitable	168:175	arg1	materials					177:185	suitable materials	168:185	suitable materials	168:185	Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies.					
35678531	2	10	theme	plasma-treated	510:523	arg1	liquids					525:531	plasma-treated liquids	510:531	plasma-treated liquids	510:531	Acting as delivery vehicles that allow a controlled release of RONS to the diseased site, plasma-treated hydrogels can overcome some of the limitations presented by plasma-treated liquids in in vivo therapies.					
35678531	0	11	theme	Thermosensitive	0:14	arg1	hydrogels					16:24	Thermosensitive hydrogels	0:24	Thermosensitive hydrogels	0:24	Thermosensitive hydrogels to deliver reactive species generated by cold atmospheric plasma: a case study with methylcellulose.					
35678531	3	12	theme	physiological	680:692	arg1	temperatures					694:705	physiological temperatures	680:705	physiological temperatures	680:705	In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS.					
35678531	4	13	theme	MC	843:844	arg1	hydrogels					846:854	MC hydrogels	843:854	MC hydrogels	843:854	MC hydrogels demonstrated the capacity for generation, prolonged storage and release of RONS.					
35678531	0	14	with	study	99:103	arg1	methylcellulose					110:124	methylcellulose	110:124	methylcellulose	110:124	Thermosensitive hydrogels to deliver reactive species generated by cold atmospheric plasma: a case study with methylcellulose.					
35678531	2	15	theme	controlled	386:395	arg1	release					397:403	a controlled release	384:403	a controlled release of RONS to the diseased site	384:432	Acting as delivery vehicles that allow a controlled release of RONS to the diseased site, plasma-treated hydrogels can overcome some of the limitations presented by plasma-treated liquids in in vivo therapies.					
35678531	2	16	theme	plasma-treated	435:448	arg1	hydrogels					450:458	plasma-treated hydrogels	435:458	plasma-treated hydrogels	435:458	Acting as delivery vehicles that allow a controlled release of RONS to the diseased site, plasma-treated hydrogels can overcome some of the limitations presented by plasma-treated liquids in in vivo therapies.					
35678531	3	17	theme	liquid	730:735	arg1	phase					737:741	the liquid phase	726:741	the liquid phase	726:741	In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS.					
35678531	2	18	theme	diseased	420:427	arg1	site					429:432	the diseased site	416:432	the diseased site	416:432	Acting as delivery vehicles that allow a controlled release of RONS to the diseased site, plasma-treated hydrogels can overcome some of the limitations presented by plasma-treated liquids in in vivo therapies.					
35678531	6	19	theme	good	1153:1156	arg1	hydrogels					1140:1148	plasma-treated thermosensitive hydrogels	1109:1148	plasma-treated thermosensitive hydrogels	1109:1148	These promising results postulate plasma-treated thermosensitive hydrogels as good candidates to provide local anticancer therapies.					
35678531	6	19	theme	good	1153:1156	arg1	candidates					1158:1167	good candidates	1153:1167	good candidates	1153:1167	These promising results postulate plasma-treated thermosensitive hydrogels as good candidates to provide local anticancer therapies.					
35678531	1	20	theme	promising	288:296	arg1	Hydrogels					127:135	Hydrogels	127:135	Hydrogels	127:135	Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies.					
35678531	1	20	theme	promising	288:296	arg1	alternatives					298:309	promising alternatives	288:309	promising alternatives to conventional cancer therapies	288:342	Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies.					
35678531	2	21	dep	in	536:537	arg1	vivo					539:542	vivo	539:542	vivo	539:542	Acting as delivery vehicles that allow a controlled release of RONS to the diseased site, plasma-treated hydrogels can overcome some of the limitations presented by plasma-treated liquids in in vivo therapies.					
35678531	5	22	theme	dose-response	1053:1065	arg1	manner					1067:1072	a dose-response manner	1051:1072	a dose-response manner	1051:1072	This release induced cytotoxic effects on the osteosarcoma cancer cell line MG-63, reducing its cell viability in a dose-response manner.					
35678531	5	23	theme	cell	1003:1006	arg1	line					1008:1011	the osteosarcoma cancer cell line MG-63	979:1017	the osteosarcoma cancer cell line MG-63	979:1017	This release induced cytotoxic effects on the osteosarcoma cancer cell line MG-63, reducing its cell viability in a dose-response manner.					
35678531	0	24	theme	reactive	37:44	arg1	species					46:52	reactive species	37:52	reactive species generated by cold atmospheric plasma	37:89	Thermosensitive hydrogels to deliver reactive species generated by cold atmospheric plasma: a case study with methylcellulose.					
35678531	3	25	theme	room	746:749	arg1	temperature					751:761	room temperature	746:761	room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS	746:840	In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS.					
35678531	2	26	theme	RONS	408:411	arg1	release					397:403	a controlled release	384:403	a controlled release of RONS to the diseased site	384:432	Acting as delivery vehicles that allow a controlled release of RONS to the diseased site, plasma-treated hydrogels can overcome some of the limitations presented by plasma-treated liquids in in vivo therapies.					
35678531	2	27	theme	in	536:537	arg1	therapies					544:552	in vivo therapies	536:552	in vivo therapies	536:552	Acting as delivery vehicles that allow a controlled release of RONS to the diseased site, plasma-treated hydrogels can overcome some of the limitations presented by plasma-treated liquids in in vivo therapies.					
35678531	3	28	theme	hydrogel	624:631	arg1	composition					586:596	the composition	582:596	the composition of a methylcellulose (MC) hydrogel	582:631	In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS.					
35678531	1	29	theme	conventional	314:325	arg1	therapies					334:342	conventional cancer therapies	314:342	conventional cancer therapies	314:342	Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies.					
35678531	1	30	theme	cancer	327:332	arg1	therapies					334:342	conventional cancer therapies	314:342	conventional cancer therapies	314:342	Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies.					
35678531	4	31	theme	prolonged	898:906	arg1	storage					908:914	prolonged storage	898:914	prolonged storage	898:914	MC hydrogels demonstrated the capacity for generation, prolonged storage and release of RONS.					
35678531	5	32	theme	cell	1033:1036	arg1	viability					1038:1046	its cell viability	1029:1046	its cell viability	1029:1046	This release induced cytotoxic effects on the osteosarcoma cancer cell line MG-63, reducing its cell viability in a dose-response manner.					
35678531	0	33	dep	deliver	29:35	arg1	study					99:103	a case study	92:103	a case study with methylcellulose	92:124	Thermosensitive hydrogels to deliver reactive species generated by cold atmospheric plasma: a case study with methylcellulose.					
35678531	6	34	theme	thermosensitive	1124:1138	arg1	hydrogels					1140:1148	plasma-treated thermosensitive hydrogels	1109:1148	plasma-treated thermosensitive hydrogels	1109:1148	These promising results postulate plasma-treated thermosensitive hydrogels as good candidates to provide local anticancer therapies.					
35678531	6	34	theme	thermosensitive	1124:1138	arg1	candidates					1158:1167	good candidates	1153:1167	good candidates	1153:1167	These promising results postulate plasma-treated thermosensitive hydrogels as good candidates to provide local anticancer therapies.					
35678531	3	35	theme	gas-plasma	772:781	arg1	treatment					783:791	gas-plasma treatment	772:791	gas-plasma treatment with suitable formation of plasma-generated RONS	772:840	In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS.					
35678531	6	36	theme	local	1180:1184	arg1	therapies					1197:1205	local anticancer therapies	1180:1205	local anticancer therapies	1180:1205	These promising results postulate plasma-treated thermosensitive hydrogels as good candidates to provide local anticancer therapies.					
35678531	5	37	theme	osteosarcoma	983:994	arg1	line					1008:1011	the osteosarcoma cancer cell line MG-63	979:1017	the osteosarcoma cancer cell line MG-63	979:1017	This release induced cytotoxic effects on the osteosarcoma cancer cell line MG-63, reducing its cell viability in a dose-response manner.					
35678531	6	38	theme	plasma-treated	1109:1122	arg1	hydrogels					1140:1148	plasma-treated thermosensitive hydrogels	1109:1148	plasma-treated thermosensitive hydrogels	1109:1148	These promising results postulate plasma-treated thermosensitive hydrogels as good candidates to provide local anticancer therapies.					
35678531	6	38	theme	plasma-treated	1109:1122	arg1	candidates					1158:1167	good candidates	1153:1167	good candidates	1153:1167	These promising results postulate plasma-treated thermosensitive hydrogels as good candidates to provide local anticancer therapies.					
35678531	3	39	theme	plasma-generated	820:835	arg1	RONS					837:840	plasma-generated RONS	820:840	plasma-generated RONS	820:840	In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS.					
35678531	0	40	theme	atmospheric	72:82	arg1	plasma					84:89	cold atmospheric plasma	67:89	cold atmospheric plasma	67:89	Thermosensitive hydrogels to deliver reactive species generated by cold atmospheric plasma: a case study with methylcellulose.					
35678531	1	41	theme	nitrogen	219:226	arg1	species					228:234	reactive oxygen and nitrogen species	199:234	species	228:234	Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies.					
35678531	5	42	theme	cancer	996:1001	arg1	line					1008:1011	the osteosarcoma cancer cell line MG-63	979:1017	the osteosarcoma cancer cell line MG-63	979:1017	This release induced cytotoxic effects on the osteosarcoma cancer cell line MG-63, reducing its cell viability in a dose-response manner.					
35678531	1	43	theme	reactive	199:206	arg1	RONS					237:240	RONS	237:240	RONS	237:240	Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies.					
35678531	1	43	theme	reactive	199:206	arg1	oxygen					208:213	reactive oxygen and nitrogen species	199:234	oxygen	208:213	Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies.					
35678531	3	44	theme	RONS	837:840	arg1	formation					807:815	suitable formation	798:815	suitable formation of plasma-generated RONS	798:840	In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS.					
35678531	0	45	theme	cold	67:70	arg1	plasma					84:89	cold atmospheric plasma	67:89	cold atmospheric plasma	67:89	Thermosensitive hydrogels to deliver reactive species generated by cold atmospheric plasma: a case study with methylcellulose.					
35678531	3	46	theme	methylcellulose	603:617	arg1	hydrogel					624:631	a methylcellulose (MC) hydrogel	601:631	a methylcellulose (MC) hydrogel	601:631	In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS.					
35569715	7	0	contain	have	854:857	arg2	influence					873:881	a significant influence	859:881	a significant influence	859:881	The pH conditions, in contrast to HRT, were found to have a significant influence on the bacterial composition.					
35569715	7	0	contain	have	854:857	arg1	conditions					808:817	The pH conditions	801:817	The pH conditions	801:817	The pH conditions, in contrast to HRT, were found to have a significant influence on the bacterial composition.					
35569715	8	1	theme	H2	1010:1011	arg1	performance					1024:1034	stable and high H2 production performance	994:1034	stable and high H2 production performance	994:1034	Low diversity in bacterial culture dominated by the Clostridium genus allows for stable and high H2 production performance.					
35569715	4	2	theme	L	600:600	arg1	H2/L-d					602:607	11.4 L H2/L-d	595:607	11.4 L H2/L-d	595:607	At a HRT of 3 h it reached 11.4 L H2/L-d.					
35569715	7	3	theme	bacterial	890:898	arg1	composition					900:910	the bacterial composition	886:910	the bacterial composition	886:910	The pH conditions, in contrast to HRT, were found to have a significant influence on the bacterial composition.					
35569715	3	4	theme	production	465:474	arg1	rate					476:479	The highest hydrogen production rate	444:479	The highest hydrogen production rate between HRTs of 24 and 12 h	444:507	The highest hydrogen production rate between HRTs of 24 and 12 h was observed at pH 6.5, while at HRT below 12 h at pH 6.0.					
35569715	0	5	theme	bacterial	91:99	arg1	culture					101:107	a mixed bacterial culture	83:107	a mixed bacterial culture	83:107	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.					
35569715	3	6	from	pH	560:561	arg1	12 h					552:555	12 h	552:555	12 h at pH 6.0	552:565	The highest hydrogen production rate between HRTs of 24 and 12 h was observed at pH 6.5, while at HRT below 12 h at pH 6.0.					
35569715	1	7	from	production	302:311	arg1	bioreactors					321:331	four bioreactors	316:331	four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5	316:368	This study assessed the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5.					
35569715	7	8	from	conditions	808:817	arg1	contrast					823:830	contrast	823:830	contrast to HRT	823:837	The pH conditions, in contrast to HRT, were found to have a significant influence on the bacterial composition.					
35569715	5	9	theme	optimum	620:626	arg1	pH					628:629	the optimum pH	616:629	the optimum pH for H2 production	616:647	Thus, the optimum pH for H2 production depends on the HRT.					
35569715	6	10	theme	sugar	681:685	arg1	utilization					687:697	The highest sugar utilization	669:697	The highest sugar utilization	669:697	The highest sugar utilization was obtained at pH 6.0 and 6.5 and decreased in the following order: cellobiose > xylose > arabinose.					
35569715	3	11	theme	12 h	504:507	arg1	HRTs					489:492	HRTs	489:492	HRTs of 24 and 12 h	489:507	The highest hydrogen production rate between HRTs of 24 and 12 h was observed at pH 6.5, while at HRT below 12 h at pH 6.0.					
35569715	0	12	from	hydrolysate	68:78	arg1	production					27:36	Dark-fermentative hydrogen production	0:36	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.	0:177	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.					
35569715	2	13	theme	cellobiose	384:393	arg1	mixture					373:379	A mixture	371:379	A mixture of cellobiose, xylose and arabinose	371:415	A mixture of cellobiose, xylose and arabinose was used as the substrate.					
35569715	2	13	theme	cellobiose	384:393	arg1	substrate					433:441	the substrate	429:441	the substrate	429:441	A mixture of cellobiose, xylose and arabinose was used as the substrate.					
35569715	8	14	theme	Low	913:915	arg1	diversity					917:925	Low diversity	913:925	Low diversity in bacterial culture dominated by the Clostridium genus	913:981	Low diversity in bacterial culture dominated by the Clostridium genus allows for stable and high H2 production performance.					
35569715	2	15	theme	arabinose	407:415	arg1	mixture					373:379	A mixture	371:379	A mixture of cellobiose, xylose and arabinose	371:415	A mixture of cellobiose, xylose and arabinose was used as the substrate.					
35569715	2	15	theme	arabinose	407:415	arg1	substrate					433:441	the substrate	429:441	the substrate	429:441	A mixture of cellobiose, xylose and arabinose was used as the substrate.					
35569715	3	16	located	observed	513:520	arg2	rate					476:479	The highest hydrogen production rate	444:479	The highest hydrogen production rate between HRTs of 24 and 12 h	444:507	The highest hydrogen production rate between HRTs of 24 and 12 h was observed at pH 6.5, while at HRT below 12 h at pH 6.0.					
35569715	3	16	located	observed	513:520	arg1	pH					525:526	pH 6.5	525:530	pH 6.5	525:530	The highest hydrogen production rate between HRTs of 24 and 12 h was observed at pH 6.5, while at HRT below 12 h at pH 6.0.					
35569715	0	17	theme	hydrogen	18:25	arg1	production					27:36	Dark-fermentative hydrogen production	0:36	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.	0:177	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.					
35569715	8	18	theme	high	1005:1008	arg1	performance					1024:1034	stable and high H2 production performance	994:1034	stable and high H2 production performance	994:1034	Low diversity in bacterial culture dominated by the Clostridium genus allows for stable and high H2 production performance.					
35569715	8	19	theme	bacterial	930:938	arg1	culture					940:946	bacterial culture	930:946	bacterial culture dominated by the Clostridium genus	930:981	Low diversity in bacterial culture dominated by the Clostridium genus allows for stable and high H2 production performance.					
35569715	5	20	theme	H2	635:636	arg1	production					638:647	H2 production	635:647	H2 production	635:647	Thus, the optimum pH for H2 production depends on the HRT.					
35569715	6	21	theme	following	751:759	arg1	order					761:765	the following order	747:765	the following order	747:765	The highest sugar utilization was obtained at pH 6.0 and 6.5 and decreased in the following order: cellobiose > xylose > arabinose.					
35569715	4	22	theme	3 h	580:582	arg1	HRT					573:575	a HRT	571:575	a HRT of 3 h	571:582	At a HRT of 3 h it reached 11.4 L H2/L-d.					
35569715	0	23	theme	Dark-fermentative	0:16	arg1	production					27:36	Dark-fermentative hydrogen production	0:36	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.	0:177	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.					
35569715	8	24	theme	Clostridium	965:975	arg1	genus					977:981	the Clostridium genus	961:981	the Clostridium genus	961:981	Low diversity in bacterial culture dominated by the Clostridium genus allows for stable and high H2 production performance.					
35569715	7	25	theme	pH	805:806	arg1	conditions					808:817	The pH conditions	801:817	The pH conditions	801:817	The pH conditions, in contrast to HRT, were found to have a significant influence on the bacterial composition.					
35569715	1	26	theme	continuous	270:279	arg1	production					302:311	continuous dark-fermentative H2 production	270:311	continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5	270:368	This study assessed the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5.					
35569715	0	27	theme	retention	145:153	arg1	time					155:158	hydraulic retention time	135:158	hydraulic retention time	135:158	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.					
35569715	6	28	theme	highest	673:679	arg1	utilization					687:697	The highest sugar utilization	669:697	The highest sugar utilization	669:697	The highest sugar utilization was obtained at pH 6.0 and 6.5 and decreased in the following order: cellobiose > xylose > arabinose.					
35569715	2	29	theme	xylose	396:401	arg1	mixture					373:379	A mixture	371:379	A mixture of cellobiose, xylose and arabinose	371:415	A mixture of cellobiose, xylose and arabinose was used as the substrate.					
35569715	2	29	theme	xylose	396:401	arg1	substrate					433:441	the substrate	429:441	the substrate	429:441	A mixture of cellobiose, xylose and arabinose was used as the substrate.					
35569715	1	30	theme	dark-fermentative	281:297	arg1	production					302:311	continuous dark-fermentative H2 production	270:311	continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5	270:368	This study assessed the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5.					
35569715	0	31	theme	hydraulic	135:143	arg1	time					155:158	hydraulic retention time	135:158	hydraulic retention time	135:158	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.					
35569715	1	32	theme	hydraulic	213:221	arg1	HRT					239:241	HRT	239:241	HRT	239:241	This study assessed the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5.					
35569715	1	32	theme	hydraulic	213:221	arg1	time					233:236	hydraulic retention time	213:236	hydraulic retention time (HRT) ranging from 24 to 3 h	213:265	This study assessed the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5.					
35569715	0	33	theme	lignocellulose	53:66	arg1	hydrolysate					68:78	synthetic lignocellulose hydrolysate	43:78	synthetic lignocellulose hydrolysate	43:78	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.					
35569715	1	34	theme	H2	299:300	arg1	production					302:311	continuous dark-fermentative H2 production	270:311	continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5	270:368	This study assessed the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5.					
35569715	1	35	theme	retention	223:231	arg1	HRT					239:241	HRT	239:241	HRT	239:241	This study assessed the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5.					
35569715	1	35	theme	retention	223:231	arg1	time					233:236	hydraulic retention time	213:236	hydraulic retention time (HRT) ranging from 24 to 3 h	213:265	This study assessed the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5.					
35569715	0	36	theme	synthetic	43:51	arg1	hydrolysate					68:78	synthetic lignocellulose hydrolysate	43:78	synthetic lignocellulose hydrolysate	43:78	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.					
35569715	1	37	theme	time	233:236	arg1	effect					203:208	the effect	199:208	the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5	199:368	This study assessed the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5.					
35569715	3	38	theme	highest	448:454	arg1	rate					476:479	The highest hydrogen production rate	444:479	The highest hydrogen production rate between HRTs of 24 and 12 h	444:507	The highest hydrogen production rate between HRTs of 24 and 12 h was observed at pH 6.5, while at HRT below 12 h at pH 6.0.					
35569715	8	39	from	diversity	917:925	arg1	culture					940:946	bacterial culture	930:946	bacterial culture dominated by the Clostridium genus	930:981	Low diversity in bacterial culture dominated by the Clostridium genus allows for stable and high H2 production performance.					
35569715	0	40	theme	pH	164:165	arg1	conditions					167:176	pH conditions	164:176	pH conditions	164:176	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.					
35569715	3	41	theme	hydrogen	456:463	arg1	rate					476:479	The highest hydrogen production rate	444:479	The highest hydrogen production rate between HRTs of 24 and 12 h	444:507	The highest hydrogen production rate between HRTs of 24 and 12 h was observed at pH 6.5, while at HRT below 12 h at pH 6.0.					
35569715	1	42	dep	3 h	263:265	arg1	to					260:261	to	260:261	to	260:261	This study assessed the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5.					
35569715	7	43	theme	significant	861:871	arg1	influence					873:881	a significant influence	859:881	a significant influence	859:881	The pH conditions, in contrast to HRT, were found to have a significant influence on the bacterial composition.					
35569715	3	44	theme	24	497:498	arg1	HRTs					489:492	HRTs	489:492	HRTs of 24 and 12 h	489:507	The highest hydrogen production rate between HRTs of 24 and 12 h was observed at pH 6.5, while at HRT below 12 h at pH 6.0.					
35569715	0	45	dep	production	27:36	arg1	relationship					114:125	The relationship	110:125	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.	0:177	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.					
35569715	0	46	theme	mixed	85:89	arg1	culture					101:107	a mixed bacterial culture	83:107	a mixed bacterial culture	83:107	Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions.					
35569715	1	47	from	effect	203:208	arg1	production					302:311	continuous dark-fermentative H2 production	270:311	continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5	270:368	This study assessed the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5.					
35569715	8	48	theme	stable	994:999	arg1	performance					1024:1034	stable and high H2 production performance	994:1034	stable and high H2 production performance	994:1034	Low diversity in bacterial culture dominated by the Clostridium genus allows for stable and high H2 production performance.					
35569715	2	49	used	used	421:424	arg2	substrate					433:441	the substrate	429:441	the substrate	429:441	A mixture of cellobiose, xylose and arabinose was used as the substrate.					
35569715	2	49	used	used	421:424	arg2	mixture					373:379	A mixture	371:379	A mixture of cellobiose, xylose and arabinose	371:415	A mixture of cellobiose, xylose and arabinose was used as the substrate.					
35569715	8	50	theme	production	1013:1022	arg1	performance					1024:1034	stable and high H2 production performance	994:1034	stable and high H2 production performance	994:1034	Low diversity in bacterial culture dominated by the Clostridium genus allows for stable and high H2 production performance.					
36056292	6	0	theme	butanoate	1483:1491	arg1	metabolism					1493:1502	the potential biomarker-related butanoate metabolism	1451:1502	the potential biomarker-related butanoate metabolism	1451:1502	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	0	theme	butanoate	1483:1491	arg1	metabolites					1407:1417	feces metabolites	1401:1417	feces metabolites in LPS-treated mice	1401:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	5	1	theme	propionic	1207:1215	arg1	acids					1263:1267	the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids	1197:1267	the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids	1197:1267	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	4	2	theme	cecal	812:816	arg1	levels					849:854	The cecal short-chain fatty acid (SCFAs) levels	808:854	The cecal short-chain fatty acid (SCFAs) levels	808:854	The cecal short-chain fatty acid (SCFAs) levels were increased in LPS-treated mice with L. paracasei CCFM1222 pretreatment.					
36056292	8	3	theme	inflammatory	2031:2042	arg1	responses					2044:2052	the inflammatory responses	2027:2052	the inflammatory responses	2027:2052	In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses and altering intestinal microbiota composition and their metabolites.					
36056292	6	4	theme	tryptophan	1568:1577	arg1	metabolites					1407:1417	feces metabolites	1401:1417	feces metabolites in LPS-treated mice	1401:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	4	theme	tryptophan	1568:1577	arg1	metabolism					1579:1588	tryptophan metabolism	1568:1588	tryptophan metabolism	1568:1588	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	1	5	theme	acute	177:181	arg1	ALI					197:199	ALI	197:199	ALI	197:199	In recent years, acute liver injury (ALI) has received wide-range attention in the world due to its relatively high morbidity and mortality.					
36056292	1	5	theme	acute	177:181	arg1	injury					189:194	acute liver injury	177:194	acute liver injury (ALI)	177:200	In recent years, acute liver injury (ALI) has received wide-range attention in the world due to its relatively high morbidity and mortality.					
36056292	4	6	theme	fatty	830:834	arg1	SCFAs					842:846	SCFAs	842:846	SCFAs	842:846	The cecal short-chain fatty acid (SCFAs) levels were increased in LPS-treated mice with L. paracasei CCFM1222 pretreatment.					
36056292	4	6	theme	fatty	830:834	arg1	acid					836:839	short-chain fatty acid	818:839	The cecal short-chain fatty acid (SCFAs) levels	808:854	The cecal short-chain fatty acid (SCFAs) levels were increased in LPS-treated mice with L. paracasei CCFM1222 pretreatment.					
36056292	8	7	theme	L.	1900:1901	arg1	supplementation					1922:1936	L. paracasei CCFM1222 supplementation	1900:1936	L. paracasei CCFM1222 supplementation	1900:1936	In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses and altering intestinal microbiota composition and their metabolites.					
36056292	3	8	theme	L.	582:583	arg1	pretreatment					604:615	L. paracasei CCFM1222 pretreatment	582:615	L. paracasei CCFM1222 pretreatment	582:615	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	3	9	theme	CAT	760:762	arg1	activities					776:785	the SOD, CAT, and GSH-Px activities	751:785	the SOD, CAT, and GSH-Px activities in LPS-treated mice	751:805	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	2	10	theme	metabolomics	541:552	arg1	perspective					498:508	the perspective	494:508	the perspective of intestinal microbiomics and metabolomics	494:552	This study aimed to explore the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice and further elaborate its mechanism of action from the perspective of intestinal microbiomics and metabolomics.					
36056292	7	11	theme	paracasei	1682:1690	arg1	pretreatment					1701:1712	L. paracasei CCFM1222 pretreatment	1679:1712	L. paracasei CCFM1222 pretreatment	1679:1712	Moreover, L. paracasei CCFM1222 pretreatment remarkably regulated the expression of gene-associated ALI (including Tlr4, Myd88, Nf-kβ, iNOS, Cox2, Iκ-Bα, Nrf2, and Sirt-1).					
36056292	6	12	theme	L.	1323:1324	arg1	pretreatment					1345:1356	L. paracasei CCFM1222 pretreatment	1323:1356	L. paracasei CCFM1222 pretreatment	1323:1356	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	5	13	theme	higher	1052:1057	arg1	abundance					1059:1067	the higher abundance	1048:1067	the higher abundance of Faecalibaculum	1048:1085	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	4	14	theme	L.	896:897	arg1	pretreatment					918:929	L. paracasei CCFM1222 pretreatment	896:929	L. paracasei CCFM1222 pretreatment	896:929	The cecal short-chain fatty acid (SCFAs) levels were increased in LPS-treated mice with L. paracasei CCFM1222 pretreatment.					
36056292	3	15	theme	CCFM1222	595:602	arg1	pretreatment					604:615	L. paracasei CCFM1222 pretreatment	582:615	L. paracasei CCFM1222 pretreatment	582:615	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	8	16	theme	CCFM1222	1913:1920	arg1	supplementation					1922:1936	L. paracasei CCFM1222 supplementation	1900:1936	L. paracasei CCFM1222 supplementation	1900:1936	In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses and altering intestinal microbiota composition and their metabolites.					
36056292	3	17	theme	GSH-Px	769:774	arg1	activities					776:785	the SOD, CAT, and GSH-Px activities	751:785	the SOD, CAT, and GSH-Px activities in LPS-treated mice	751:805	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	6	18	from	mice	1434:1437	arg1	composition					1386:1396	the composition	1382:1396	the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism	1382:1666	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	19	theme	arginine	1612:1619	arg1	metabolites					1407:1417	feces metabolites	1401:1417	feces metabolites in LPS-treated mice	1401:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	19	theme	arginine	1612:1619	arg1	biosynthesis					1621:1632	arginine biosynthesis	1612:1632	arginine biosynthesis	1612:1632	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	5	20	theme	cecal	1201:1205	arg1	acids					1263:1267	the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids	1197:1267	the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids	1197:1267	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	6	21	from	composition	1386:1396	arg1	mice					1434:1437	LPS-treated mice	1422:1437	LPS-treated mice	1422:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	2	22	theme	action	482:487	arg1	mechanism					469:477	its mechanism	465:477	its mechanism of action	465:487	This study aimed to explore the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice and further elaborate its mechanism of action from the perspective of intestinal microbiomics and metabolomics.					
36056292	5	23	theme	isobutyric	1236:1245	arg1	acids					1263:1267	the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids	1197:1267	the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids	1197:1267	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	0	24	theme	Lactobacillus	87:99	arg1	CCFM1222					111:118	Lactobacillus paracasei CCFM1222	87:118	Lactobacillus paracasei CCFM1222	87:118	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.					
36056292	7	25	theme	ALI	1769:1771	arg1	expression					1739:1748	the expression	1735:1748	the expression of gene-associated ALI (including Tlr4, Myd88, Nf-kβ, iNOS, Cox2, Iκ-Bα, Nrf2, and Sirt-1)	1735:1839	Moreover, L. paracasei CCFM1222 pretreatment remarkably regulated the expression of gene-associated ALI (including Tlr4, Myd88, Nf-kβ, iNOS, Cox2, Iκ-Bα, Nrf2, and Sirt-1).					
36056292	2	26	theme	microbiomics	524:535	arg1	perspective					498:508	the perspective	494:508	the perspective of intestinal microbiomics and metabolomics	494:552	This study aimed to explore the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice and further elaborate its mechanism of action from the perspective of intestinal microbiomics and metabolomics.					
36056292	0	27	theme	CCFM1222	111:118	arg1	Effects					76:82	the Hepatoprotective Effects	55:82	the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice	55:157	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.					
36056292	6	28	theme	CCFM1222	1336:1343	arg1	pretreatment					1345:1356	L. paracasei CCFM1222 pretreatment	1323:1356	L. paracasei CCFM1222 pretreatment	1323:1356	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	5	29	theme	L.	945:946	arg1	pretreatment					967:978	L. paracasei CCFM1222 pretreatment	945:978	L. paracasei CCFM1222 pretreatment	945:978	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	0	30	theme	Intestinal	0:9	arg1	Insights					41:48	Intestinal Microbiomics and Metabolomics Insights	0:48	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.	0:158	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.					
36056292	6	31	theme	proline	1649:1655	arg1	metabolism					1657:1666	proline metabolism	1649:1666	proline metabolism	1649:1666	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	31	theme	proline	1649:1655	arg1	metabolites					1407:1417	feces metabolites	1401:1417	feces metabolites in LPS-treated mice	1401:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	0	32	dep	Lactobacillus	87:99	arg1	paracasei					101:109	paracasei	101:109	paracasei	101:109	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.					
36056292	1	33	theme	high	271:274	arg1	morbidity					276:284	its relatively high morbidity	256:284	its relatively high morbidity	256:284	In recent years, acute liver injury (ALI) has received wide-range attention in the world due to its relatively high morbidity and mortality.					
36056292	7	34	theme	CCFM1222	1692:1699	arg1	pretreatment					1701:1712	L. paracasei CCFM1222 pretreatment	1679:1712	L. paracasei CCFM1222 pretreatment	1679:1712	Moreover, L. paracasei CCFM1222 pretreatment remarkably regulated the expression of gene-associated ALI (including Tlr4, Myd88, Nf-kβ, iNOS, Cox2, Iκ-Bα, Nrf2, and Sirt-1).					
36056292	6	35	theme	LPS-treated	1422:1432	arg1	mice					1434:1437	LPS-treated mice	1422:1437	LPS-treated mice	1422:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	5	36	theme	Prevotellaceae	1132:1145	arg1	group					1154:1158	the Prevotellaceae NK3B31 group	1128:1158	the Prevotellaceae NK3B31 group	1128:1158	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	8	37	theme	intestinal	2067:2076	arg1	composition					2089:2099	intestinal microbiota composition	2067:2099	intestinal microbiota composition	2067:2099	In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses and altering intestinal microbiota composition and their metabolites.					
36056292	6	38	theme	metabolites	1407:1417	arg1	composition					1386:1396	the composition	1382:1396	the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism	1382:1666	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	5	39	theme	group	1154:1158	arg1	abundance					1059:1067	the higher abundance	1048:1067	the higher abundance of Faecalibaculum	1048:1085	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	5	39	theme	group	1154:1158	arg1	Bifidobacterium					1088:1102	Bifidobacterium	1088:1102	Bifidobacterium	1088:1102	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	5	39	theme	group	1154:1158	arg1	abundance					1115:1123	lower abundance	1109:1123	lower abundance of the Prevotellaceae NK3B31 group	1109:1158	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	5	40	theme	lower	1109:1113	arg1	abundance					1115:1123	lower abundance	1109:1123	lower abundance of the Prevotellaceae NK3B31 group	1109:1158	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	5	41	theme	microbiota	1014:1023	arg1	composition					1025:1035	the intestinal microbiota composition	999:1035	the intestinal microbiota composition	999:1035	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	5	42	theme	CCFM1222	958:965	arg1	pretreatment					967:978	L. paracasei CCFM1222 pretreatment	945:978	L. paracasei CCFM1222 pretreatment	945:978	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	6	43	theme	vitamin	1505:1511	arg1	metabolites					1407:1417	feces metabolites	1401:1417	feces metabolites in LPS-treated mice	1401:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	43	theme	vitamin	1505:1511	arg1	metabolism					1516:1525	vitamin B6 metabolism	1505:1525	vitamin B6 metabolism	1505:1525	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	2	44	theme	hepatoprotective	333:348	arg1	effect					350:355	the hepatoprotective effect	329:355	the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice	329:441	This study aimed to explore the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice and further elaborate its mechanism of action from the perspective of intestinal microbiomics and metabolomics.					
36056292	4	45	theme	acid	836:839	arg1	levels					849:854	The cecal short-chain fatty acid (SCFAs) levels	808:854	The cecal short-chain fatty acid (SCFAs) levels	808:854	The cecal short-chain fatty acid (SCFAs) levels were increased in LPS-treated mice with L. paracasei CCFM1222 pretreatment.					
36056292	3	46	theme	hepatic	698:704	arg1	TNF-α					706:710	hepatic TNF-α	698:710	hepatic TNF-α	698:710	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	6	47	theme	biomarker-related	1465:1481	arg1	metabolism					1493:1502	the potential biomarker-related butanoate metabolism	1451:1502	the potential biomarker-related butanoate metabolism	1451:1502	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	47	theme	biomarker-related	1465:1481	arg1	metabolites					1407:1417	feces metabolites	1401:1417	feces metabolites in LPS-treated mice	1401:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	3	48	dep	the	682:684	arg1	releases					686:693	releases	686:693	releases	686:693	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	3	49	theme	IL-6	724:727	arg1	levels					729:734	IL-6 levels	724:734	IL-6 levels	724:734	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	8	50	theme	paracasei	1903:1911	arg1	supplementation					1922:1936	L. paracasei CCFM1222 supplementation	1900:1936	L. paracasei CCFM1222 supplementation	1900:1936	In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses and altering intestinal microbiota composition and their metabolites.					
36056292	1	51	theme	liver	183:187	arg1	ALI					197:199	ALI	197:199	ALI	197:199	In recent years, acute liver injury (ALI) has received wide-range attention in the world due to its relatively high morbidity and mortality.					
36056292	1	51	theme	liver	183:187	arg1	injury					189:194	acute liver injury	177:194	acute liver injury (ALI)	177:200	In recent years, acute liver injury (ALI) has received wide-range attention in the world due to its relatively high morbidity and mortality.					
36056292	7	52	theme	L.	1679:1680	arg1	pretreatment					1701:1712	L. paracasei CCFM1222 pretreatment	1679:1712	L. paracasei CCFM1222 pretreatment	1679:1712	Moreover, L. paracasei CCFM1222 pretreatment remarkably regulated the expression of gene-associated ALI (including Tlr4, Myd88, Nf-kβ, iNOS, Cox2, Iκ-Bα, Nrf2, and Sirt-1).					
36056292	4	53	theme	short-chain	818:828	arg1	SCFAs					842:846	SCFAs	842:846	SCFAs	842:846	The cecal short-chain fatty acid (SCFAs) levels were increased in LPS-treated mice with L. paracasei CCFM1222 pretreatment.					
36056292	4	53	theme	short-chain	818:828	arg1	acid					836:839	short-chain fatty acid	818:839	The cecal short-chain fatty acid (SCFAs) levels	808:854	The cecal short-chain fatty acid (SCFAs) levels were increased in LPS-treated mice with L. paracasei CCFM1222 pretreatment.					
36056292	6	54	theme	paracasei	1326:1334	arg1	pretreatment					1345:1356	L. paracasei CCFM1222 pretreatment	1323:1356	L. paracasei CCFM1222 pretreatment	1323:1356	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	55	theme	D-glutamate	1544:1554	arg1	metabolites					1407:1417	feces metabolites	1401:1417	feces metabolites in LPS-treated mice	1401:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	55	theme	D-glutamate	1544:1554	arg1	metabolism					1556:1565	D-glutamine and D-glutamate metabolism	1528:1565	metabolism	1556:1565	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	4	56	theme	LPS-treated	874:884	arg1	mice					886:889	LPS-treated mice	874:889	LPS-treated mice	874:889	The cecal short-chain fatty acid (SCFAs) levels were increased in LPS-treated mice with L. paracasei CCFM1222 pretreatment.					
36056292	8	57	theme	beneficial	1942:1951	arg1	effects					1953:1959	beneficial effects	1942:1959	beneficial effects	1942:1959	In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses and altering intestinal microbiota composition and their metabolites.					
36056292	6	58	theme	D-glutamine	1528:1538	arg1	metabolites					1407:1417	feces metabolites	1401:1417	feces metabolites in LPS-treated mice	1401:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	58	theme	D-glutamine	1528:1538	arg1	metabolism					1556:1565	D-glutamine and D-glutamate metabolism	1528:1565	metabolism	1556:1565	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	4	59	theme	CCFM1222	909:916	arg1	pretreatment					918:929	L. paracasei CCFM1222 pretreatment	896:929	L. paracasei CCFM1222 pretreatment	896:929	The cecal short-chain fatty acid (SCFAs) levels were increased in LPS-treated mice with L. paracasei CCFM1222 pretreatment.					
36056292	1	60	from	attention	226:234	arg1	world					243:247	the world	239:247	the world	239:247	In recent years, acute liver injury (ALI) has received wide-range attention in the world due to its relatively high morbidity and mortality.					
36056292	5	61	theme	Faecalibaculum	1072:1085	arg1	abundance					1059:1067	the higher abundance	1048:1067	the higher abundance of Faecalibaculum	1048:1085	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	5	61	theme	Faecalibaculum	1072:1085	arg1	Bifidobacterium					1088:1102	Bifidobacterium	1088:1102	Bifidobacterium	1088:1102	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	5	61	theme	Faecalibaculum	1072:1085	arg1	abundance					1115:1123	lower abundance	1109:1123	lower abundance of the Prevotellaceae NK3B31 group	1109:1158	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	3	62	theme	paracasei	585:593	arg1	pretreatment					604:615	L. paracasei CCFM1222 pretreatment	582:615	L. paracasei CCFM1222 pretreatment	582:615	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	8	63	dep	occurrence	1979:1988	arg1	the					1975:1977	the	1975:1977	the	1975:1977	In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses and altering intestinal microbiota composition and their metabolites.					
36056292	5	64	theme	isovaleric	1252:1261	arg1	acids					1263:1267	the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids	1197:1267	the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids	1197:1267	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	3	65	theme	SOD	755:757	arg1	activities					776:785	the SOD, CAT, and GSH-Px activities	751:785	the SOD, CAT, and GSH-Px activities in LPS-treated mice	751:805	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	6	66	from	metabolites	1407:1417	arg1	mice					1434:1437	LPS-treated mice	1422:1437	LPS-treated mice	1422:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	3	67	theme	serum	645:649	arg1	ALT					651:653	the serum ALT, and AST levels	641:669	ALT	651:653	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	6	68	theme	feces	1401:1405	arg1	metabolism					1657:1666	proline metabolism	1649:1666	proline metabolism	1649:1666	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	68	theme	feces	1401:1405	arg1	metabolism					1579:1588	tryptophan metabolism	1568:1588	tryptophan metabolism	1568:1588	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	68	theme	feces	1401:1405	arg1	biosynthesis					1621:1632	arginine biosynthesis	1612:1632	arginine biosynthesis	1612:1632	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	68	theme	feces	1401:1405	arg1	metabolism					1493:1502	the potential biomarker-related butanoate metabolism	1451:1502	the potential biomarker-related butanoate metabolism	1451:1502	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	68	theme	feces	1401:1405	arg1	arginine					1635:1642	arginine	1635:1642	arginine	1635:1642	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	68	theme	feces	1401:1405	arg1	metabolism					1556:1565	D-glutamine and D-glutamate metabolism	1528:1565	metabolism	1556:1565	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	68	theme	feces	1401:1405	arg1	metabolism					1600:1609	caffeine metabolism	1591:1609	caffeine metabolism	1591:1609	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	68	theme	feces	1401:1405	arg1	metabolites					1407:1417	feces metabolites	1401:1417	feces metabolites in LPS-treated mice	1401:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	68	theme	feces	1401:1405	arg1	metabolism					1516:1525	vitamin B6 metabolism	1505:1525	vitamin B6 metabolism	1505:1525	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	8	69	contain	has	1938:1940	arg1	supplementation					1922:1936	L. paracasei CCFM1222 supplementation	1900:1936	L. paracasei CCFM1222 supplementation	1900:1936	In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses and altering intestinal microbiota composition and their metabolites.					
36056292	8	69	contain	has	1938:1940	arg2	effects					1953:1959	beneficial effects	1942:1959	beneficial effects	1942:1959	In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses and altering intestinal microbiota composition and their metabolites.					
36056292	2	70	theme	intestinal	513:522	arg1	microbiomics					524:535	intestinal microbiomics	513:535	intestinal microbiomics	513:535	This study aimed to explore the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice and further elaborate its mechanism of action from the perspective of intestinal microbiomics and metabolomics.					
36056292	6	71	theme	caffeine	1591:1598	arg1	metabolites					1407:1417	feces metabolites	1401:1417	feces metabolites in LPS-treated mice	1401:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	71	theme	caffeine	1591:1598	arg1	metabolism					1600:1609	caffeine metabolism	1591:1609	caffeine metabolism	1591:1609	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	1	72	theme	recent	163:168	arg1	years					170:174	recent years	163:174	recent years	163:174	In recent years, acute liver injury (ALI) has received wide-range attention in the world due to its relatively high morbidity and mortality.					
36056292	0	73	theme	Liver	138:142	arg1	Injury					144:149	the Acute Liver Injury	128:149	the Acute Liver Injury in Mice	128:157	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.					
36056292	4	74	theme	paracasei	899:907	arg1	pretreatment					918:929	L. paracasei CCFM1222 pretreatment	896:929	L. paracasei CCFM1222 pretreatment	896:929	The cecal short-chain fatty acid (SCFAs) levels were increased in LPS-treated mice with L. paracasei CCFM1222 pretreatment.					
36056292	3	75	theme	LPS-treated	790:800	arg1	mice					802:805	LPS-treated mice	790:805	LPS-treated mice	790:805	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	0	76	theme	Acute	132:136	arg1	Injury					144:149	the Acute Liver Injury	128:149	the Acute Liver Injury in Mice	128:157	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.					
36056292	8	77	theme	ALI	2009:2011	arg1	development					1994:2004	development	1994:2004	development	1994:2004	In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses and altering intestinal microbiota composition and their metabolites.					
36056292	8	77	theme	ALI	2009:2011	arg1	occurrence					1979:1988	occurrence	1979:1988	occurrence	1979:1988	In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses and altering intestinal microbiota composition and their metabolites.					
36056292	7	78	theme	gene-associated	1753:1767	arg1	ALI					1769:1771	gene-associated ALI	1753:1771	gene-associated ALI (including Tlr4, Myd88, Nf-kβ, iNOS, Cox2, Iκ-Bα, Nrf2, and Sirt-1)	1753:1839	Moreover, L. paracasei CCFM1222 pretreatment remarkably regulated the expression of gene-associated ALI (including Tlr4, Myd88, Nf-kβ, iNOS, Cox2, Iκ-Bα, Nrf2, and Sirt-1).					
36056292	5	79	theme	butyric	1218:1224	arg1	acids					1263:1267	the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids	1197:1267	the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids	1197:1267	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	2	80	theme	CCFM1222	384:391	arg1	mice					438:441	Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice	360:441	Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice	360:441	This study aimed to explore the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice and further elaborate its mechanism of action from the perspective of intestinal microbiomics and metabolomics.					
36056292	0	81	theme	Microbiomics	11:22	arg1	Insights					41:48	Intestinal Microbiomics and Metabolomics Insights	0:48	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.	0:158	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.					
36056292	2	82	theme	mice	438:441	arg1	effect					350:355	the hepatoprotective effect	329:355	the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice	329:441	This study aimed to explore the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice and further elaborate its mechanism of action from the perspective of intestinal microbiomics and metabolomics.					
36056292	2	83	theme	Lactobacillus	360:372	arg1	CCFM1222					384:391	Lactobacillus paracasei CCFM1222	360:391	Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice	360:441	This study aimed to explore the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice and further elaborate its mechanism of action from the perspective of intestinal microbiomics and metabolomics.					
36056292	0	84	theme	Metabolomics	28:39	arg1	Insights					41:48	Intestinal Microbiomics and Metabolomics Insights	0:48	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.	0:158	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.					
36056292	2	85	theme	-induced	425:432	arg1	ALI					434:436	lipopolysaccharide (LPS)-induced ALI	401:436	lipopolysaccharide (LPS)-induced ALI	401:436	This study aimed to explore the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice and further elaborate its mechanism of action from the perspective of intestinal microbiomics and metabolomics.					
36056292	3	86	theme	AST	660:662	arg1	levels					664:669	the serum ALT, and AST levels	641:669	levels	664:669	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	8	87	theme	microbiota	2078:2087	arg1	composition					2089:2099	intestinal microbiota composition	2067:2099	intestinal microbiota composition	2067:2099	In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses and altering intestinal microbiota composition and their metabolites.					
36056292	0	88	theme	Hepatoprotective	59:74	arg1	Effects					76:82	the Hepatoprotective Effects	55:82	the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice	55:157	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.					
36056292	5	89	theme	NK3B31	1147:1152	arg1	group					1154:1158	the Prevotellaceae NK3B31 group	1128:1158	the Prevotellaceae NK3B31 group	1128:1158	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	5	90	theme	intestinal	1003:1012	arg1	composition					1025:1035	the intestinal microbiota composition	999:1035	the intestinal microbiota composition	999:1035	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	5	91	theme	paracasei	948:956	arg1	pretreatment					967:978	L. paracasei CCFM1222 pretreatment	945:978	L. paracasei CCFM1222 pretreatment	945:978	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
36056292	3	92	from	activities	776:785	arg1	mice					802:805	LPS-treated mice	790:805	LPS-treated mice	790:805	The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice.					
36056292	2	93	dep	Lactobacillus	360:372	arg1	paracasei					374:382	paracasei	374:382	paracasei	374:382	This study aimed to explore the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice and further elaborate its mechanism of action from the perspective of intestinal microbiomics and metabolomics.					
36056292	6	94	theme	B6	1513:1514	arg1	metabolites					1407:1417	feces metabolites	1401:1417	feces metabolites in LPS-treated mice	1401:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	94	theme	B6	1513:1514	arg1	metabolism					1516:1525	vitamin B6 metabolism	1505:1525	vitamin B6 metabolism	1505:1525	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	1	95	theme	wide-range	215:224	arg1	attention					226:234	wide-range attention	215:234	wide-range attention in the world due to its relatively high morbidity and mortality	215:298	In recent years, acute liver injury (ALI) has received wide-range attention in the world due to its relatively high morbidity and mortality.					
36056292	0	96	from	Injury	144:149	arg1	Mice					154:157	Mice	154:157	Mice	154:157	Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice.					
36056292	6	97	theme	potential	1455:1463	arg1	metabolism					1493:1502	the potential biomarker-related butanoate metabolism	1451:1502	the potential biomarker-related butanoate metabolism	1451:1502	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	6	97	theme	potential	1455:1463	arg1	metabolites					1407:1417	feces metabolites	1401:1417	feces metabolites in LPS-treated mice	1401:1437	The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarker-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism.					
36056292	5	98	theme	valeric	1227:1233	arg1	acids					1263:1267	the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids	1197:1267	the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids	1197:1267	In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of the Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids.					
35153010	0	0	theme	drug	113:116	arg1	delivery					118:125	drug delivery	113:125	drug delivery	113:125	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite NPs as mucoadhesive carrier for drug delivery.					
35153010	8	1	used	used	1166:1169	arg2	NPs					1153:1155	M-N-2-HACC/N-CMCS NPs	1135:1155	M-N-2-HACC/N-CMCS NPs	1135:1155	Collectively, these results suggested that M-N-2-HACC/N-CMCS NPs could be used as a promising candidate for mucosal delivery.					
35153010	8	1	used	used	1166:1169	arg2	candidate					1186:1194	a promising candidate	1174:1194	a promising candidate for mucosal delivery	1174:1215	Collectively, these results suggested that M-N-2-HACC/N-CMCS NPs could be used as a promising candidate for mucosal delivery.					
35153010	2	2	theme	bifunctional	347:358	arg1	NPs					388:390	NPs	388:390	NPs	388:390	In the present study, we aimed to synthesize bifunctional mucoadhesive nanoparticles (NPs) that could be used for mucosal delivery.					
35153010	2	2	theme	bifunctional	347:358	arg1	nanoparticles					373:385	bifunctional mucoadhesive nanoparticles	347:385	bifunctional mucoadhesive nanoparticles (NPs) that could be used for mucosal delivery	347:431	In the present study, we aimed to synthesize bifunctional mucoadhesive nanoparticles (NPs) that could be used for mucosal delivery.					
35153010	6	3	theme	administration	1010:1023	arg1	effectiveness					985:997	the effectiveness	981:997	the effectiveness of mucosal administration	981:1023	Moreover, it demonstrated high hydrophilicity, enduring drug release, stability, safety, and mucosal adhesion, which contributed to the effectiveness of mucosal administration.					
35153010	5	4	theme	particle	776:783	arg1	size					785:788	a particle size	774:788	a particle size of 196.72 ± 0.45 nm and zeta potential of 17.12 ± 0.50 mV	774:846	The NPs showed a particle size of 196.72 ± 0.45 nm and zeta potential of 17.12 ± 0.50 mV.					
35153010	1	5	theme	drug	185:188	arg1	delivery					173:180	the mucosal delivery	161:180	the mucosal delivery of drug	161:188	There are various challenges for the mucosal delivery of drug, which is largely attributed to the absence of effective drug carriers that can make delivery to mucosal sites.					
35153010	6	6	theme	mucosal	1002:1008	arg1	administration					1010:1023	mucosal administration	1002:1023	mucosal administration	1002:1023	Moreover, it demonstrated high hydrophilicity, enduring drug release, stability, safety, and mucosal adhesion, which contributed to the effectiveness of mucosal administration.					
35153010	8	7	theme	promising	1176:1184	arg1	candidate					1186:1194	a promising candidate	1174:1194	a promising candidate for mucosal delivery	1174:1215	Collectively, these results suggested that M-N-2-HACC/N-CMCS NPs could be used as a promising candidate for mucosal delivery.					
35153010	8	7	theme	promising	1176:1184	arg1	NPs					1153:1155	M-N-2-HACC/N-CMCS NPs	1135:1155	M-N-2-HACC/N-CMCS NPs	1135:1155	Collectively, these results suggested that M-N-2-HACC/N-CMCS NPs could be used as a promising candidate for mucosal delivery.					
35153010	8	8	theme	mucosal	1200:1206	arg1	delivery					1208:1215	mucosal delivery	1200:1215	mucosal delivery	1200:1215	Collectively, these results suggested that M-N-2-HACC/N-CMCS NPs could be used as a promising candidate for mucosal delivery.					
35153010	4	9	theme	thiolated	711:719	arg1	NPs					730:732	mannose-modified thiolated chitosan NPs	694:732	mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs)	694:756	The electrostatic interaction between the two substances was used to produce mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs).					
35153010	4	9	theme	thiolated	711:719	arg1	NPs					753:755	M-N-2-HACC/N-CMCS NPs	735:755	M-N-2-HACC/N-CMCS NPs	735:755	The electrostatic interaction between the two substances was used to produce mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs).					
35153010	5	10	theme	zeta	814:817	arg1	potential					819:827	zeta potential	814:827	zeta potential	814:827	The NPs showed a particle size of 196.72 ± 0.45 nm and zeta potential of 17.12 ± 0.50 mV.					
35153010	6	11	theme	drug	905:908	arg1	release					910:916	enduring drug release	896:916	enduring drug release	896:916	Moreover, it demonstrated high hydrophilicity, enduring drug release, stability, safety, and mucosal adhesion, which contributed to the effectiveness of mucosal administration.					
35153010	5	12	theme	potential	819:827	arg1	size					785:788	a particle size	774:788	a particle size of 196.72 ± 0.45 nm and zeta potential of 17.12 ± 0.50 mV	774:846	The NPs showed a particle size of 196.72 ± 0.45 nm and zeta potential of 17.12 ± 0.50 mV.					
35153010	3	13	mod	modified	506:513	arg1	M-N-2-HACC					490:499	M-N-2-HACC	490:499	M-N-2-HACC	490:499	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	3	13	mod	modified	506:513	arg3	D-mannose					520:528	D-mannose	520:528	D-mannose	520:528	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	3	13	mod	modified	506:513	arg1	chitosan					480:487	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan	434:487	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC)	434:500	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	0	14	theme	quaternized	17:27	arg1	composite					71:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite	0:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite	0:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite NPs as mucoadhesive carrier for drug delivery.					
35153010	6	15	theme	enduring	896:903	arg1	release					910:916	enduring drug release	896:916	enduring drug release	896:916	Moreover, it demonstrated high hydrophilicity, enduring drug release, stability, safety, and mucosal adhesion, which contributed to the effectiveness of mucosal administration.					
35153010	0	16	theme	Mannose-anchored	0:15	arg1	composite					71:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite	0:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite	0:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite NPs as mucoadhesive carrier for drug delivery.					
35153010	2	17	theme	present	309:315	arg1	study					317:321	the present study	305:321	the present study	305:321	In the present study, we aimed to synthesize bifunctional mucoadhesive nanoparticles (NPs) that could be used for mucosal delivery.					
35153010	0	18	theme	carboxymethyl	48:60	arg1	composite					71:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite	0:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite	0:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite NPs as mucoadhesive carrier for drug delivery.					
35153010	1	19	theme	mucosal	287:293	arg1	sites					295:299	mucosal sites	287:299	mucosal sites	287:299	There are various challenges for the mucosal delivery of drug, which is largely attributed to the absence of effective drug carriers that can make delivery to mucosal sites.					
35153010	3	20	theme	chloride	471:478	arg1	M-N-2-HACC					490:499	M-N-2-HACC	490:499	M-N-2-HACC	490:499	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	3	20	theme	chloride	471:478	arg1	chitosan					480:487	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan	434:487	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC)	434:500	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	2	21	theme	mucosal	416:422	arg1	delivery					424:431	mucosal delivery	416:431	mucosal delivery	416:431	In the present study, we aimed to synthesize bifunctional mucoadhesive nanoparticles (NPs) that could be used for mucosal delivery.					
35153010	4	22	theme	mannose-modified	694:709	arg1	NPs					730:732	mannose-modified thiolated chitosan NPs	694:732	mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs)	694:756	The electrostatic interaction between the two substances was used to produce mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs).					
35153010	4	22	theme	mannose-modified	694:709	arg1	NPs					753:755	M-N-2-HACC/N-CMCS NPs	735:755	M-N-2-HACC/N-CMCS NPs	735:755	The electrostatic interaction between the two substances was used to produce mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs).					
35153010	4	23	theme	electrostatic	621:633	arg1	interaction					635:645	The electrostatic interaction	617:645	The electrostatic interaction between the two substances	617:672	The electrostatic interaction between the two substances was used to produce mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs).					
35153010	3	24	theme	trimethyl	452:460	arg1	M-N-2-HACC					490:499	M-N-2-HACC	490:499	M-N-2-HACC	490:499	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	3	24	theme	trimethyl	452:460	arg1	chitosan					480:487	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan	434:487	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC)	434:500	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	0	25	theme	chitosan	62:69	arg1	composite					71:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite	0:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite	0:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite NPs as mucoadhesive carrier for drug delivery.					
35153010	6	26	theme	high	875:878	arg1	hydrophilicity					880:893	high hydrophilicity	875:893	high hydrophilicity	875:893	Moreover, it demonstrated high hydrophilicity, enduring drug release, stability, safety, and mucosal adhesion, which contributed to the effectiveness of mucosal administration.					
35153010	3	27	theme	ammonium	462:469	arg1	M-N-2-HACC					490:499	M-N-2-HACC	490:499	M-N-2-HACC	490:499	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	3	27	theme	ammonium	462:469	arg1	chitosan					480:487	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan	434:487	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC)	434:500	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	5	28	theme	196.72 ± 0.45 nm	793:808	arg1	size					785:788	a particle size	774:788	a particle size of 196.72 ± 0.45 nm and zeta potential of 17.12 ± 0.50 mV	774:846	The NPs showed a particle size of 196.72 ± 0.45 nm and zeta potential of 17.12 ± 0.50 mV.					
35153010	4	29	used	used	678:681	arg2	interaction					635:645	The electrostatic interaction	617:645	The electrostatic interaction between the two substances	617:672	The electrostatic interaction between the two substances was used to produce mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs).					
35153010	1	30	theme	various	138:144	arg1	challenges					146:155	various challenges	138:155	various challenges	138:155	There are various challenges for the mucosal delivery of drug, which is largely attributed to the absence of effective drug carriers that can make delivery to mucosal sites.					
35153010	6	31	theme	mucosal	942:948	arg1	adhesion					950:957	mucosal adhesion	942:957	mucosal adhesion	942:957	Moreover, it demonstrated high hydrophilicity, enduring drug release, stability, safety, and mucosal adhesion, which contributed to the effectiveness of mucosal administration.					
35153010	3	32	theme	carboxymethyl	584:596	arg1	chitosan					598:605	the carboxymethyl chitosan	580:605	the carboxymethyl chitosan (N-CMCS)	580:614	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	3	32	theme	carboxymethyl	584:596	arg1	N-CMCS					608:613	N-CMCS	608:613	N-CMCS	608:613	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	2	33	used	used	407:410	arg2	nanoparticles					373:385	bifunctional mucoadhesive nanoparticles	347:385	bifunctional mucoadhesive nanoparticles (NPs) that could be used for mucosal delivery	347:431	In the present study, we aimed to synthesize bifunctional mucoadhesive nanoparticles (NPs) that could be used for mucosal delivery.					
35153010	2	33	used	used	407:410	arg2	NPs					388:390	NPs	388:390	NPs	388:390	In the present study, we aimed to synthesize bifunctional mucoadhesive nanoparticles (NPs) that could be used for mucosal delivery.					
35153010	4	34	theme	chitosan	721:728	arg1	NPs					730:732	mannose-modified thiolated chitosan NPs	694:732	mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs)	694:756	The electrostatic interaction between the two substances was used to produce mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs).					
35153010	4	34	theme	chitosan	721:728	arg1	NPs					753:755	M-N-2-HACC/N-CMCS NPs	735:755	M-N-2-HACC/N-CMCS NPs	735:755	The electrostatic interaction between the two substances was used to produce mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs).					
35153010	4	35	theme	M-N-2-HACC/N-CMCS	735:751	arg1	NPs					730:732	mannose-modified thiolated chitosan NPs	694:732	mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs)	694:756	The electrostatic interaction between the two substances was used to produce mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs).					
35153010	4	35	theme	M-N-2-HACC/N-CMCS	735:751	arg1	NPs					753:755	M-N-2-HACC/N-CMCS NPs	735:755	M-N-2-HACC/N-CMCS NPs	735:755	The electrostatic interaction between the two substances was used to produce mannose-modified thiolated chitosan NPs (M-N-2-HACC/N-CMCS NPs).					
35153010	8	36	theme	M-N-2-HACC/N-CMCS	1135:1151	arg1	candidate					1186:1194	a promising candidate	1174:1194	a promising candidate for mucosal delivery	1174:1215	Collectively, these results suggested that M-N-2-HACC/N-CMCS NPs could be used as a promising candidate for mucosal delivery.					
35153010	8	36	theme	M-N-2-HACC/N-CMCS	1135:1151	arg1	NPs					1153:1155	M-N-2-HACC/N-CMCS NPs	1135:1155	M-N-2-HACC/N-CMCS NPs	1135:1155	Collectively, these results suggested that M-N-2-HACC/N-CMCS NPs could be used as a promising candidate for mucosal delivery.					
35153010	5	37	theme	17.12 ± 0.50 mV	832:846	arg1	196.72 ± 0.45 nm					793:808	196.72 ± 0.45 nm	793:808	196.72 ± 0.45 nm	793:808	The NPs showed a particle size of 196.72 ± 0.45 nm and zeta potential of 17.12 ± 0.50 mV.					
35153010	5	37	theme	17.12 ± 0.50 mV	832:846	arg1	potential					819:827	zeta potential	814:827	zeta potential	814:827	The NPs showed a particle size of 196.72 ± 0.45 nm and zeta potential of 17.12 ± 0.50 mV.					
35153010	1	38	theme	effective	237:245	arg1	carriers					252:259	effective drug carriers	237:259	effective drug carriers that can make delivery to mucosal sites	237:299	There are various challenges for the mucosal delivery of drug, which is largely attributed to the absence of effective drug carriers that can make delivery to mucosal sites.					
35153010	1	39	theme	mucosal	165:171	arg1	delivery					173:180	the mucosal delivery	161:180	the mucosal delivery of drug	161:188	There are various challenges for the mucosal delivery of drug, which is largely attributed to the absence of effective drug carriers that can make delivery to mucosal sites.					
35153010	3	40	theme	N-2-Hydroxypropyl	434:450	arg1	M-N-2-HACC					490:499	M-N-2-HACC	490:499	M-N-2-HACC	490:499	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	3	40	theme	N-2-Hydroxypropyl	434:450	arg1	chitosan					480:487	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan	434:487	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC)	434:500	N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (M-N-2-HACC) was modified with D-mannose, and N-acetyl-L-cysteine (NAC) was immobilized on the carboxymethyl chitosan (N-CMCS).					
35153010	1	41	theme	drug	247:250	arg1	carriers					252:259	effective drug carriers	237:259	effective drug carriers that can make delivery to mucosal sites	237:299	There are various challenges for the mucosal delivery of drug, which is largely attributed to the absence of effective drug carriers that can make delivery to mucosal sites.					
35153010	0	42	theme	mucoadhesive	88:99	arg1	carrier					101:107	mucoadhesive carrier	88:107	mucoadhesive carrier for drug delivery	88:125	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite NPs as mucoadhesive carrier for drug delivery.					
35153010	0	43	theme	chitosan/thiolated	29:46	arg1	composite					71:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite	0:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite	0:79	Mannose-anchored quaternized chitosan/thiolated carboxymethyl chitosan composite NPs as mucoadhesive carrier for drug delivery.					
35153010	2	44	theme	mucoadhesive	360:371	arg1	NPs					388:390	NPs	388:390	NPs	388:390	In the present study, we aimed to synthesize bifunctional mucoadhesive nanoparticles (NPs) that could be used for mucosal delivery.					
35153010	2	44	theme	mucoadhesive	360:371	arg1	nanoparticles					373:385	bifunctional mucoadhesive nanoparticles	347:385	bifunctional mucoadhesive nanoparticles (NPs) that could be used for mucosal delivery	347:431	In the present study, we aimed to synthesize bifunctional mucoadhesive nanoparticles (NPs) that could be used for mucosal delivery.					
35153010	1	45	theme	carriers	252:259	arg1	absence					226:232	the absence	222:232	the absence of effective drug carriers that can make delivery to mucosal sites	222:299	There are various challenges for the mucosal delivery of drug, which is largely attributed to the absence of effective drug carriers that can make delivery to mucosal sites.					
36370856	6	0	theme	higher	1103:1108	arg1	antioxidant					1110:1120	a higher antioxidant	1101:1120	a higher antioxidant (>90%)	1101:1127	Furthermore, the as-prepared nanocomposites exhibited a higher antioxidant (>90%) and antibacterial activity.					
36370856	6	0	theme	higher	1103:1108	arg1	%					1126:1126	>90%	1123:1126	>90%	1123:1126	Furthermore, the as-prepared nanocomposites exhibited a higher antioxidant (>90%) and antibacterial activity.					
36370856	7	1	attach	derived	1252:1258	arg2	nanocomposites					1237:1250	the nanocomposites	1233:1250	the nanocomposites derived from different clay minerals	1233:1287	Due to the synergistic effect of each component among the nanocompositions, the nanocomposites derived from different clay minerals were employed as multifunctional nanofillers to design functional chitosan composite films.					
36370856	7	1	attach	derived	1252:1258	arg2	nanofillers					1322:1332	multifunctional nanofillers	1306:1332	multifunctional nanofillers	1306:1332	Due to the synergistic effect of each component among the nanocompositions, the nanocomposites derived from different clay minerals were employed as multifunctional nanofillers to design functional chitosan composite films.					
36370856	7	1	attach	derived	1252:1258	arg1	minerals					1280:1287	different clay minerals	1265:1287	different clay minerals	1265:1287	Due to the synergistic effect of each component among the nanocompositions, the nanocomposites derived from different clay minerals were employed as multifunctional nanofillers to design functional chitosan composite films.					
36370856	2	2	theme	silver	357:362	arg1	nanoparticles					364:376	silver nanoparticles	357:376	silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent	357:430	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	2	2	theme	silver	357:362	arg1	AgNPs					379:383	AgNPs	379:383	AgNPs	379:383	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	4	3	theme	or/and	878:883	arg1	interlayer					885:894	the surface or/and interlayer	866:894	the surface or/and interlayer of the involved clay minerals	866:924	Meanwhile, incorporation of clay minerals could induce the in-situ heterogeneous nucleation of AgNPs on the surface or/and interlayer of the involved clay minerals.					
36370856	11	4	dep	resistance	1951:1960	arg1	water					1945:1949	water	1945:1949	water	1945:1949	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	4	5	theme	AgNPs	857:861	arg1	nucleation					843:852	the in-situ heterogeneous nucleation	817:852	the in-situ heterogeneous nucleation of AgNPs	817:861	Meanwhile, incorporation of clay minerals could induce the in-situ heterogeneous nucleation of AgNPs on the surface or/and interlayer of the involved clay minerals.					
36370856	1	6	theme	green	207:211	arg1	reducing					213:220	green reducing and end-capping reagent	207:244	reducing	213:220	It is popular that natural organics are served as green reducing and end-capping reagent for synthesis of functional nanoparticles.					
36370856	4	7	theme	minerals	917:924	arg1	interlayer					885:894	the surface or/and interlayer	866:894	the surface or/and interlayer of the involved clay minerals	866:924	Meanwhile, incorporation of clay minerals could induce the in-situ heterogeneous nucleation of AgNPs on the surface or/and interlayer of the involved clay minerals.					
36370856	11	8	theme	obtained	1867:1874	arg1	film					1886:1889	the obtained composite film	1863:1889	the obtained composite film with the ginger color	1863:1911	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	2	9	from	approach	472:479	arg1	presence					488:495	the presence	484:495	the presence of different clay minerals	484:522	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	11	10	theme	potential	2044:2052	arg1	materials					2075:2083	a great potential functional packaging materials	2036:2083	a great potential functional packaging materials	2036:2083	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	2	11	theme	palygorskite	587:598	arg1	clay					600:603	mixed-dimensional palygorskite clay	569:603	mixed-dimensional palygorskite clay	569:603	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	8	12	theme	mechanical	1526:1535	arg1	antioxidant					1549:1559	antioxidant	1549:1559	antioxidant	1549:1559	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	8	12	theme	mechanical	1526:1535	arg1	activities					1579:1588	antibacterial activities	1565:1588	antibacterial activities	1565:1588	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	8	12	theme	mechanical	1526:1535	arg1	properties					1537:1546	the best mechanical properties	1517:1546	the best mechanical properties	1517:1546	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	9	13	dep	strength	1637:1644	arg1	the					1625:1627	the	1625:1627	the	1625:1627	Compared with the chitosan films, the tensile strength and elongation at break of composite films increased by 15.90 MPa and 27.27%, respectively.					
36370856	5	14	theme	30-40 nm	1037:1044	arg1	diameter					1025:1032	a diameter	1023:1032	a diameter of 30-40 nm	1023:1044	It effectively prevented from the aggregations and resulted in uniform dispersion of AgNPs with a diameter of 30-40 nm.					
36370856	9	15	from	break	1664:1668	arg1	at					1661:1662	elongation at break	1650:1668	elongation at break	1650:1668	Compared with the chitosan films, the tensile strength and elongation at break of composite films increased by 15.90 MPa and 27.27%, respectively.					
36370856	9	15	from	break	1664:1668	arg1	elongation					1650:1659	elongation at break	1650:1668	elongation at break	1650:1668	Compared with the chitosan films, the tensile strength and elongation at break of composite films increased by 15.90 MPa and 27.27%, respectively.					
36370856	4	16	theme	involved	903:910	arg1	minerals					917:924	the involved clay minerals	899:924	the involved clay minerals	899:924	Meanwhile, incorporation of clay minerals could induce the in-situ heterogeneous nucleation of AgNPs on the surface or/and interlayer of the involved clay minerals.					
36370856	3	17	theme	phenolic	628:635	arg1	curcumin					675:682	curcumin	675:682	curcumin	675:682	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	3	17	theme	phenolic	628:635	arg1	groups					646:651	the phenolic hydroxyl groups	624:651	the phenolic hydroxyl groups	624:651	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	11	18	theme	packaging	2065:2073	arg1	materials					2075:2083	a great potential functional packaging materials	2036:2083	a great potential functional packaging materials	2036:2083	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	9	19	theme	chitosan	1609:1616	arg1	films					1618:1622	the chitosan films	1605:1622	the chitosan films	1605:1622	Compared with the chitosan films, the tensile strength and elongation at break of composite films increased by 15.90 MPa and 27.27%, respectively.					
36370856	0	20	theme	mechanical	94:103	arg1	properties					105:114	mechanical properties	94:114	mechanical properties	94:114	Incorporation of silver nanoparticles/curcumin/clay minerals into chitosan film for enhancing mechanical properties, antioxidant and antibacterial activity.					
36370856	2	21	theme	minerals	515:522	arg1	presence					488:495	the presence	484:495	the presence of different clay minerals	484:522	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	9	22	theme	tensile	1629:1635	arg1	strength					1637:1644	tensile strength	1629:1644	tensile strength	1629:1644	Compared with the chitosan films, the tensile strength and elongation at break of composite films increased by 15.90 MPa and 27.27%, respectively.					
36370856	2	23	theme	different	500:508	arg1	minerals					515:522	different clay minerals	500:522	different clay minerals	500:522	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	2	24	theme	coloring	391:398	arg1	agent					426:430	a coloring, reducing and end-capping agent	389:430	a coloring, reducing and end-capping agent	389:430	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	3	25	with	AgNPs	733:737	arg1	color					755:759	the ginger color	744:759	the ginger color	744:759	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	7	26	theme	composite	1364:1372	arg1	films					1374:1378	functional chitosan composite films	1344:1378	functional chitosan composite films	1344:1378	Due to the synergistic effect of each component among the nanocompositions, the nanocomposites derived from different clay minerals were employed as multifunctional nanofillers to design functional chitosan composite films.					
36370856	2	27	theme	eco-friendly	438:449	arg1	approach					472:479	an eco-friendly, economic and facile approach	435:479	an eco-friendly, economic and facile approach	435:479	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	11	28	theme	antibacterial	1979:1991	arg1	properties					1993:2002	excellent mechanical, water resistance, antioxidant and antibacterial properties	1923:2002	excellent mechanical, water resistance, antioxidant and antibacterial properties	1923:2002	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	11	29	with	film	1886:1889	arg1	color					1907:1911	the ginger color	1896:1911	the ginger color	1896:1911	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	9	30	theme	at	1661:1662	arg1	break					1664:1668	elongation at break	1650:1668	elongation at break	1650:1668	Compared with the chitosan films, the tensile strength and elongation at break of composite films increased by 15.90 MPa and 27.27%, respectively.					
36370856	7	31	theme	functional	1344:1353	arg1	films					1374:1378	functional chitosan composite films	1344:1378	functional chitosan composite films	1344:1378	Due to the synergistic effect of each component among the nanocompositions, the nanocomposites derived from different clay minerals were employed as multifunctional nanofillers to design functional chitosan composite films.					
36370856	8	32	theme	composite	1407:1415	arg1	films					1417:1421	the chitosan composite films	1394:1421	the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1394:1505	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	2	33	theme	end-capping	414:424	arg1	agent					426:430	a coloring, reducing and end-capping agent	389:430	a coloring, reducing and end-capping agent	389:430	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	0	34	theme	silver	17:22	arg1	minerals					52:59	silver nanoparticles/curcumin/clay minerals	17:59	silver nanoparticles/curcumin/clay minerals	17:59	Incorporation of silver nanoparticles/curcumin/clay minerals into chitosan film for enhancing mechanical properties, antioxidant and antibacterial activity.					
36370856	2	35	theme	facile	465:470	arg1	approach					472:479	an eco-friendly, economic and facile approach	435:479	an eco-friendly, economic and facile approach	435:479	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	3	36	theme	carbonyl	656:663	arg1	curcumin					675:682	curcumin	675:682	curcumin	675:682	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	3	36	theme	carbonyl	656:663	arg1	groups					665:670	carbonyl groups	656:670	carbonyl groups	656:670	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	2	37	theme	reducing	401:408	arg1	agent					426:430	a coloring, reducing and end-capping agent	389:430	a coloring, reducing and end-capping agent	389:430	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	0	38	theme	minerals	52:59	arg1	Incorporation					0:12	Incorporation	0:12	Incorporation of silver nanoparticles/curcumin/clay minerals into chitosan film for enhancing mechanical properties, antioxidant and antibacterial activity.	0:155	Incorporation of silver nanoparticles/curcumin/clay minerals into chitosan film for enhancing mechanical properties, antioxidant and antibacterial activity.					
36370856	9	39	theme	films	1683:1687	arg1	strength					1637:1644	tensile strength	1629:1644	tensile strength	1629:1644	Compared with the chitosan films, the tensile strength and elongation at break of composite films increased by 15.90 MPa and 27.27%, respectively.					
36370856	9	39	theme	films	1683:1687	arg1	break					1664:1668	elongation at break	1650:1668	elongation at break	1650:1668	Compared with the chitosan films, the tensile strength and elongation at break of composite films increased by 15.90 MPa and 27.27%, respectively.					
36370856	7	40	theme	multifunctional	1306:1320	arg1	nanofillers					1322:1332	multifunctional nanofillers	1306:1332	multifunctional nanofillers	1306:1332	Due to the synergistic effect of each component among the nanocompositions, the nanocomposites derived from different clay minerals were employed as multifunctional nanofillers to design functional chitosan composite films.					
36370856	7	40	theme	multifunctional	1306:1320	arg1	nanocomposites					1237:1250	the nanocomposites	1233:1250	the nanocomposites derived from different clay minerals	1233:1287	Due to the synergistic effect of each component among the nanocompositions, the nanocomposites derived from different clay minerals were employed as multifunctional nanofillers to design functional chitosan composite films.					
36370856	2	41	theme	economic	452:459	arg1	approach					472:479	an eco-friendly, economic and facile approach	435:479	an eco-friendly, economic and facile approach	435:479	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	4	42	theme	in-situ	821:827	arg1	nucleation					843:852	the in-situ heterogeneous nucleation	817:852	the in-situ heterogeneous nucleation of AgNPs	817:861	Meanwhile, incorporation of clay minerals could induce the in-situ heterogeneous nucleation of AgNPs on the surface or/and interlayer of the involved clay minerals.					
36370856	0	43	theme	chitosan	66:73	arg1	film					75:78	chitosan film	66:78	chitosan film for enhancing mechanical properties, antioxidant and antibacterial activity	66:154	Incorporation of silver nanoparticles/curcumin/clay minerals into chitosan film for enhancing mechanical properties, antioxidant and antibacterial activity.					
36370856	8	44	theme	clay	1487:1490	arg1	nanocomposites					1492:1505	curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1434:1505	curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1434:1505	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	10	45	theme	films	1777:1781	arg1	rate					1755:1758	The inactivation rate	1738:1758	The inactivation rate of the composite films against Escherichia coli and Staphylococcus aureus	1738:1832	The inactivation rate of the composite films against Escherichia coli and Staphylococcus aureus had reached 100%.					
36370856	4	46	theme	minerals	795:802	arg1	incorporation					773:785	incorporation	773:785	incorporation of clay minerals	773:802	Meanwhile, incorporation of clay minerals could induce the in-situ heterogeneous nucleation of AgNPs on the surface or/and interlayer of the involved clay minerals.					
36370856	4	46	theme	minerals	795:802	arg1	Meanwhile					762:770	Meanwhile	762:770	Meanwhile	762:770	Meanwhile, incorporation of clay minerals could induce the in-situ heterogeneous nucleation of AgNPs on the surface or/and interlayer of the involved clay minerals.					
36370856	11	47	theme	antioxidant	1963:1973	arg1	properties					1993:2002	excellent mechanical, water resistance, antioxidant and antibacterial properties	1923:2002	excellent mechanical, water resistance, antioxidant and antibacterial properties	1923:2002	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	5	48	theme	AgNPs	1012:1016	arg1	dispersion					998:1007	uniform dispersion	990:1007	uniform dispersion of AgNPs with a diameter of 30-40 nm	990:1044	It effectively prevented from the aggregations and resulted in uniform dispersion of AgNPs with a diameter of 30-40 nm.					
36370856	7	49	theme	different	1265:1273	arg1	minerals					1280:1287	different clay minerals	1265:1287	different clay minerals	1265:1287	Due to the synergistic effect of each component among the nanocompositions, the nanocomposites derived from different clay minerals were employed as multifunctional nanofillers to design functional chitosan composite films.					
36370856	11	50	theme	resistance	1951:1960	arg1	properties					1993:2002	excellent mechanical, water resistance, antioxidant and antibacterial properties	1923:2002	excellent mechanical, water resistance, antioxidant and antibacterial properties	1923:2002	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	1	51	theme	nanoparticles	274:286	arg1	synthesis					250:258	synthesis	250:258	synthesis of functional nanoparticles	250:286	It is popular that natural organics are served as green reducing and end-capping reagent for synthesis of functional nanoparticles.					
36370856	3	52	theme	crucial	693:699	arg1	role					701:704	a crucial role	691:704	a crucial role	691:704	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	8	53	theme	AgNPs/mixed-dimensional	1450:1472	arg1	nanocomposites					1492:1505	curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1434:1505	curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1434:1505	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	4	54	theme	surface	870:876	arg1	interlayer					885:894	the surface or/and interlayer	866:894	the surface or/and interlayer of the involved clay minerals	866:924	Meanwhile, incorporation of clay minerals could induce the in-situ heterogeneous nucleation of AgNPs on the surface or/and interlayer of the involved clay minerals.					
36370856	4	55	theme	heterogeneous	829:841	arg1	nucleation					843:852	the in-situ heterogeneous nucleation	817:852	the in-situ heterogeneous nucleation of AgNPs	817:861	Meanwhile, incorporation of clay minerals could induce the in-situ heterogeneous nucleation of AgNPs on the surface or/and interlayer of the involved clay minerals.					
36370856	3	56	theme	silver	716:721	arg1	ions					723:726	silver ions	716:726	silver ions	716:726	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	3	57	theme	ginger	748:753	arg1	color					755:759	the ginger color	744:759	the ginger color	744:759	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	2	58	theme	natural	316:322	arg1	curcumin					304:311	curcumin	304:311	curcumin	304:311	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	2	58	theme	natural	316:322	arg1	pigment					324:330	a natural pigment	314:330	a natural pigment	314:330	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	4	59	theme	clay	912:915	arg1	minerals					917:924	the involved clay minerals	899:924	the involved clay minerals	899:924	Meanwhile, incorporation of clay minerals could induce the in-situ heterogeneous nucleation of AgNPs on the surface or/and interlayer of the involved clay minerals.					
36370856	8	60	theme	best	1521:1524	arg1	antioxidant					1549:1559	antioxidant	1549:1559	antioxidant	1549:1559	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	8	60	theme	best	1521:1524	arg1	activities					1579:1588	antibacterial activities	1565:1588	antibacterial activities	1565:1588	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	8	60	theme	best	1521:1524	arg1	properties					1537:1546	the best mechanical properties	1517:1546	the best mechanical properties	1517:1546	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	6	61	theme	antibacterial	1133:1145	arg1	activity					1147:1154	antibacterial activity	1133:1154	antibacterial activity	1133:1154	Furthermore, the as-prepared nanocomposites exhibited a higher antioxidant (>90%) and antibacterial activity.					
36370856	11	62	theme	composite	1876:1884	arg1	film					1886:1889	the obtained composite film	1863:1889	the obtained composite film with the ginger color	1863:1911	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	11	63	theme	great	2038:2042	arg1	materials					2075:2083	a great potential functional packaging materials	2036:2083	a great potential functional packaging materials	2036:2083	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	1	64	theme	end-capping	226:236	arg1	reagent					238:244	green reducing and end-capping reagent	207:244	reagent	238:244	It is popular that natural organics are served as green reducing and end-capping reagent for synthesis of functional nanoparticles.					
36370856	2	65	theme	mixed-dimensional	569:585	arg1	clay					600:603	mixed-dimensional palygorskite clay	569:603	mixed-dimensional palygorskite clay	569:603	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	11	66	theme	ginger	1900:1905	arg1	color					1907:1911	the ginger color	1896:1911	the ginger color	1896:1911	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	8	67	theme	antibacterial	1565:1577	arg1	activities					1579:1588	antibacterial activities	1565:1588	antibacterial activities	1565:1588	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	8	67	theme	antibacterial	1565:1577	arg1	properties					1537:1546	the best mechanical properties	1517:1546	the best mechanical properties	1517:1546	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	11	68	theme	excellent	1923:1931	arg1	properties					1993:2002	excellent mechanical, water resistance, antioxidant and antibacterial properties	1923:2002	excellent mechanical, water resistance, antioxidant and antibacterial properties	1923:2002	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	11	69	theme	functional	2054:2063	arg1	materials					2075:2083	a great potential functional packaging materials	2036:2083	a great potential functional packaging materials	2036:2083	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	1	70	theme	natural	176:182	arg1	organics					184:191	natural organics	176:191	natural organics	176:191	It is popular that natural organics are served as green reducing and end-capping reagent for synthesis of functional nanoparticles.					
36370856	7	71	theme	component	1195:1203	arg1	effect					1180:1185	the synergistic effect	1164:1185	the synergistic effect of each component among the nanocompositions	1164:1230	Due to the synergistic effect of each component among the nanocompositions, the nanocomposites derived from different clay minerals were employed as multifunctional nanofillers to design functional chitosan composite films.					
36370856	2	72	theme	clay	510:513	arg1	minerals					515:522	different clay minerals	500:522	different clay minerals	500:522	In this study, curcumin, a natural pigment, was employed to prepare silver nanoparticles (AgNPs) as a coloring, reducing and end-capping agent by an eco-friendly, economic and facile approach in the presence of different clay minerals, including palygorskite, montmorillonite and mixed-dimensional palygorskite clay.					
36370856	11	73	theme	mechanical	1933:1942	arg1	properties					1993:2002	excellent mechanical, water resistance, antioxidant and antibacterial properties	1923:2002	excellent mechanical, water resistance, antioxidant and antibacterial properties	1923:2002	Therefore, the obtained composite film with the ginger color exhibited excellent mechanical, water resistance, antioxidant and antibacterial properties, and it was expected to develop a great potential functional packaging materials.					
36370856	0	74	theme	antibacterial	133:145	arg1	activity					147:154	antibacterial activity	133:154	antibacterial activity	133:154	Incorporation of silver nanoparticles/curcumin/clay minerals into chitosan film for enhancing mechanical properties, antioxidant and antibacterial activity.					
36370856	7	75	theme	chitosan	1355:1362	arg1	films					1374:1378	functional chitosan composite films	1344:1378	functional chitosan composite films	1344:1378	Due to the synergistic effect of each component among the nanocompositions, the nanocomposites derived from different clay minerals were employed as multifunctional nanofillers to design functional chitosan composite films.					
36370856	7	76	theme	synergistic	1168:1178	arg1	effect					1180:1185	the synergistic effect	1164:1185	the synergistic effect of each component among the nanocompositions	1164:1230	Due to the synergistic effect of each component among the nanocompositions, the nanocomposites derived from different clay minerals were employed as multifunctional nanofillers to design functional chitosan composite films.					
36370856	9	77	theme	elongation	1650:1659	arg1	break					1664:1668	elongation at break	1650:1668	elongation at break	1650:1668	Compared with the chitosan films, the tensile strength and elongation at break of composite films increased by 15.90 MPa and 27.27%, respectively.					
36370856	6	78	theme	as-prepared	1064:1074	arg1	nanocomposites					1076:1089	the as-prepared nanocomposites	1060:1089	the as-prepared nanocomposites	1060:1089	Furthermore, the as-prepared nanocomposites exhibited a higher antioxidant (>90%) and antibacterial activity.					
36370856	0	79	theme	nanoparticles/curcumin/clay	24:50	arg1	minerals					52:59	silver nanoparticles/curcumin/clay minerals	17:59	silver nanoparticles/curcumin/clay minerals	17:59	Incorporation of silver nanoparticles/curcumin/clay minerals into chitosan film for enhancing mechanical properties, antioxidant and antibacterial activity.					
36370856	8	80	theme	chitosan	1398:1405	arg1	films					1417:1421	the chitosan composite films	1394:1421	the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1394:1505	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	9	81	theme	composite	1673:1681	arg1	films					1683:1687	composite films	1673:1687	composite films	1673:1687	Compared with the chitosan films, the tensile strength and elongation at break of composite films increased by 15.90 MPa and 27.27%, respectively.					
36370856	3	82	theme	hydroxyl	637:644	arg1	curcumin					675:682	curcumin	675:682	curcumin	675:682	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	3	82	theme	hydroxyl	637:644	arg1	groups					646:651	the phenolic hydroxyl groups	624:651	the phenolic hydroxyl groups	624:651	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	10	83	theme	inactivation	1742:1753	arg1	rate					1755:1758	The inactivation rate	1738:1758	The inactivation rate of the composite films against Escherichia coli and Staphylococcus aureus	1738:1832	The inactivation rate of the composite films against Escherichia coli and Staphylococcus aureus had reached 100%.					
36370856	8	84	contain	containing	1423:1432	arg1	films					1417:1421	the chitosan composite films	1394:1421	the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1394:1505	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	8	84	contain	containing	1423:1432	arg2	nanocomposites					1492:1505	curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1434:1505	curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1434:1505	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	3	85	theme	curcumin	675:682	arg1	curcumin					675:682	curcumin	675:682	curcumin	675:682	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	3	85	theme	curcumin	675:682	arg1	groups					646:651	the phenolic hydroxyl groups	624:651	the phenolic hydroxyl groups	624:651	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	3	85	theme	curcumin	675:682	arg1	groups					665:670	carbonyl groups	656:670	carbonyl groups	656:670	It was found that the phenolic hydroxyl groups or carbonyl groups of curcumin played a crucial role to reduce silver ions into AgNPs with the ginger color.					
36370856	8	86	theme	curcumin-capped	1434:1448	arg1	nanocomposites					1492:1505	curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1434:1505	curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1434:1505	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	5	87	theme	uniform	990:996	arg1	dispersion					998:1007	uniform dispersion	990:1007	uniform dispersion of AgNPs with a diameter of 30-40 nm	990:1044	It effectively prevented from the aggregations and resulted in uniform dispersion of AgNPs with a diameter of 30-40 nm.					
36370856	7	88	theme	clay	1275:1278	arg1	minerals					1280:1287	different clay minerals	1265:1287	different clay minerals	1265:1287	Due to the synergistic effect of each component among the nanocompositions, the nanocomposites derived from different clay minerals were employed as multifunctional nanofillers to design functional chitosan composite films.					
36370856	8	89	theme	palygorskite	1474:1485	arg1	nanocomposites					1492:1505	curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1434:1505	curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites	1434:1505	By contrast, the chitosan composite films containing curcumin-capped AgNPs/mixed-dimensional palygorskite clay nanocomposites exhibited the best mechanical properties, antioxidant and antibacterial activities.					
36370856	10	90	theme	composite	1767:1775	arg1	films					1777:1781	the composite films	1763:1781	the composite films against Escherichia coli and Staphylococcus aureus	1763:1832	The inactivation rate of the composite films against Escherichia coli and Staphylococcus aureus had reached 100%.					
36370856	5	91	with	AgNPs	1012:1016	arg1	diameter					1025:1032	a diameter	1023:1032	a diameter of 30-40 nm	1023:1044	It effectively prevented from the aggregations and resulted in uniform dispersion of AgNPs with a diameter of 30-40 nm.					
36370856	4	92	theme	clay	790:793	arg1	minerals					795:802	clay minerals	790:802	clay minerals	790:802	Meanwhile, incorporation of clay minerals could induce the in-situ heterogeneous nucleation of AgNPs on the surface or/and interlayer of the involved clay minerals.					
36370856	1	93	theme	functional	263:272	arg1	nanoparticles					274:286	functional nanoparticles	263:286	functional nanoparticles	263:286	It is popular that natural organics are served as green reducing and end-capping reagent for synthesis of functional nanoparticles.					
37244306	8	0	theme	physical	914:921	arg1	adsorption					923:932	dominant physical adsorption	905:932	dominant physical adsorption	905:932	The change in standard enthalpy (ΔH°) of the adsorption was 44.30 kJ/mol (dominant physical adsorption).					
37244306	8	0	theme	physical	914:921	arg1	kJ/mol					897:902	44.30 kJ/mol	891:902	44.30 kJ/mol (dominant physical adsorption)	891:933	The change in standard enthalpy (ΔH°) of the adsorption was 44.30 kJ/mol (dominant physical adsorption).					
37244306	9	1	theme	Primary	936:942	arg1	mechanisms					955:964	Primary adsorption mechanisms	936:964	Primary adsorption mechanisms	936:964	Primary adsorption mechanisms were complexation, ion exchange, and Van der Waals force.					
37244306	9	2	theme	Van	1003:1005	arg1	force					1017:1021	Van der Waals force	1003:1021	Van der Waals force	1003:1021	Primary adsorption mechanisms were complexation, ion exchange, and Van der Waals force.					
37244306	11	3	dep	MBA-bead	1209:1216	arg1	1.369-3.865					1219:1229	1.369-3.865	1219:1229	1.369-3.865 US$/kg	1219:1236	The cost was estimated for producing PS-biochar (0.091 US$/kg), magnetic-biochar (0.303-0.892 US$/kg), and MBA-bead (1.369-3.865 US$/kg).					
37244306	11	4	theme	$	1198:1198	arg1	/kg					1199:1201	0.303-0.892 US$/kg	1184:1201	0.303-0.892 US$/kg	1184:1201	The cost was estimated for producing PS-biochar (0.091 US$/kg), magnetic-biochar (0.303-0.892 US$/kg), and MBA-bead (1.369-3.865 US$/kg).					
37244306	11	4	theme	$	1198:1198	arg1	magnetic-biochar					1166:1181	magnetic-biochar	1166:1181	magnetic-biochar (0.303-0.892 US$/kg)	1166:1202	The cost was estimated for producing PS-biochar (0.091 US$/kg), magnetic-biochar (0.303-0.892 US$/kg), and MBA-bead (1.369-3.865 US$/kg).					
37244306	5	5	theme	total	582:586	arg1	cm3/g					607:611	0.751 cm3/g	601:611	0.751 cm3/g	601:611	Its specific surface area (262.4 m2/g) and total pore volume (0.751 cm3/g) were obtained from nitrogen adsorption at 77K.					
37244306	5	5	theme	total	582:586	arg1	volume					593:598	total pore volume	582:598	total pore volume (0.751 cm3/g)	582:612	Its specific surface area (262.4 m2/g) and total pore volume (0.751 cm3/g) were obtained from nitrogen adsorption at 77K.					
37244306	11	6	dep	/kg	1160:1162	arg1	US					1157:1158	US	1157:1158	US	1157:1158	The cost was estimated for producing PS-biochar (0.091 US$/kg), magnetic-biochar (0.303-0.892 US$/kg), and MBA-bead (1.369-3.865 US$/kg).					
37244306	8	7	theme	dominant	905:912	arg1	adsorption					923:932	dominant physical adsorption	905:932	dominant physical adsorption	905:932	The change in standard enthalpy (ΔH°) of the adsorption was 44.30 kJ/mol (dominant physical adsorption).					
37244306	8	7	theme	dominant	905:912	arg1	kJ/mol					897:902	44.30 kJ/mol	891:902	44.30 kJ/mol (dominant physical adsorption)	891:933	The change in standard enthalpy (ΔH°) of the adsorption was 44.30 kJ/mol (dominant physical adsorption).					
37244306	4	8	theme	dried	526:530	arg1	mm					519:520	2 mm	517:520	2 mm in (dried form)	517:536	The diameter of each spherical MBA-bead was approximately 3 mm (wet form) and 2 mm in (dried form).					
37244306	4	8	theme	dried	526:530	arg1	form					532:535	dried form	526:535	dried form	526:535	The diameter of each spherical MBA-bead was approximately 3 mm (wet form) and 2 mm in (dried form).					
37244306	0	9	theme	copper	106:111	arg1	ions					113:116	copper ions	106:116	copper ions	106:116	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead as super-adsorbent for adsorbing copper ions in water media.					
37244306	5	10	theme	pore	588:591	arg1	cm3/g					607:611	0.751 cm3/g	601:611	0.751 cm3/g	601:611	Its specific surface area (262.4 m2/g) and total pore volume (0.751 cm3/g) were obtained from nitrogen adsorption at 77K.					
37244306	5	10	theme	pore	588:591	arg1	volume					593:598	total pore volume	582:598	total pore volume (0.751 cm3/g)	582:612	Its specific surface area (262.4 m2/g) and total pore volume (0.751 cm3/g) were obtained from nitrogen adsorption at 77K.					
37244306	5	11	theme	specific	543:550	arg1	m2/g					572:575	262.4 m2/g	566:575	262.4 m2/g	566:575	Its specific surface area (262.4 m2/g) and total pore volume (0.751 cm3/g) were obtained from nitrogen adsorption at 77K.					
37244306	5	11	theme	specific	543:550	arg1	area					560:563	Its specific surface area	539:563	Its specific surface area (262.4 m2/g)	539:576	Its specific surface area (262.4 m2/g) and total pore volume (0.751 cm3/g) were obtained from nitrogen adsorption at 77K.					
37244306	1	12	theme	hydrogel	258:265	arg1	MBA-bead					273:280	MBA-bead	273:280	MBA-bead	273:280	This work aimed to develop innovative material by combining properties of magnetic-biochar (derived from peanut shells) and hydrogel bead (MBA-bead) and apply it for adsorbing Cu2+ in water.					
37244306	1	12	theme	hydrogel	258:265	arg1	bead					267:270	hydrogel bead	258:270	hydrogel bead (MBA-bead)	258:281	This work aimed to develop innovative material by combining properties of magnetic-biochar (derived from peanut shells) and hydrogel bead (MBA-bead) and apply it for adsorbing Cu2+ in water.					
37244306	4	13	theme	spherical	460:468	arg1	MBA-bead					470:477	each spherical MBA-bead	455:477	each spherical MBA-bead	455:477	The diameter of each spherical MBA-bead was approximately 3 mm (wet form) and 2 mm in (dried form).					
37244306	5	14	theme	surface	552:558	arg1	m2/g					572:575	262.4 m2/g	566:575	262.4 m2/g	566:575	Its specific surface area (262.4 m2/g) and total pore volume (0.751 cm3/g) were obtained from nitrogen adsorption at 77K.					
37244306	5	14	theme	surface	552:558	arg1	area					560:563	Its specific surface area	539:563	Its specific surface area (262.4 m2/g)	539:576	Its specific surface area (262.4 m2/g) and total pore volume (0.751 cm3/g) were obtained from nitrogen adsorption at 77K.					
37244306	1	15	theme	bead	267:270	arg1	properties					194:203	properties	194:203	properties of magnetic-biochar (derived from peanut shells) and hydrogel bead (MBA-bead)	194:281	This work aimed to develop innovative material by combining properties of magnetic-biochar (derived from peanut shells) and hydrogel bead (MBA-bead) and apply it for adsorbing Cu2+ in water.					
37244306	1	16	theme	innovative	161:170	arg1	material					172:179	innovative material	161:179	innovative material	161:179	This work aimed to develop innovative material by combining properties of magnetic-biochar (derived from peanut shells) and hydrogel bead (MBA-bead) and apply it for adsorbing Cu2+ in water.					
37244306	11	17	theme	$	1233:1233	arg1	/kg					1234:1236	US$/kg	1231:1236	1.369-3.865 US$/kg	1219:1236	The cost was estimated for producing PS-biochar (0.091 US$/kg), magnetic-biochar (0.303-0.892 US$/kg), and MBA-bead (1.369-3.865 US$/kg).					
37244306	7	18	dep	mg/g	804:807	arg1	pHeq					820:823	pHeq 5.0	820:827	pHeq 5.0	820:827	Its Langmuir maximum adsorption capacity for Cu2+ was 234.1 mg/g (30 °C and pHeq 5.0).					
37244306	7	18	dep	mg/g	804:807	arg1	°C					813:814	30 °C	810:814	30 °C	810:814	Its Langmuir maximum adsorption capacity for Cu2+ was 234.1 mg/g (30 °C and pHeq 5.0).					
37244306	11	19	theme	US	1196:1197	arg1	/kg					1199:1201	0.303-0.892 US$/kg	1184:1201	0.303-0.892 US$/kg	1184:1201	The cost was estimated for producing PS-biochar (0.091 US$/kg), magnetic-biochar (0.303-0.892 US$/kg), and MBA-bead (1.369-3.865 US$/kg).					
37244306	11	19	theme	US	1196:1197	arg1	magnetic-biochar					1166:1181	magnetic-biochar	1166:1181	magnetic-biochar (0.303-0.892 US$/kg)	1166:1202	The cost was estimated for producing PS-biochar (0.091 US$/kg), magnetic-biochar (0.303-0.892 US$/kg), and MBA-bead (1.369-3.865 US$/kg).					
37244306	8	20	from	change	835:840	arg1	enthalpy					854:861	standard enthalpy	845:861	standard enthalpy (ΔH°) of the adsorption	845:885	The change in standard enthalpy (ΔH°) of the adsorption was 44.30 kJ/mol (dominant physical adsorption).					
37244306	8	20	from	change	835:840	arg1	ΔH°					864:866	ΔH°	864:866	ΔH°	864:866	The change in standard enthalpy (ΔH°) of the adsorption was 44.30 kJ/mol (dominant physical adsorption).					
37244306	0	21	theme	separable	5:13	arg1	bead					68:71	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead	0:71	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead as super-adsorbent for adsorbing copper ions in water media.	0:132	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead as super-adsorbent for adsorbing copper ions in water media.					
37244306	4	22	theme	MBA-bead	470:477	arg1	mm					499:500	approximately 3 mm	483:500	approximately 3 mm (wet form)	483:511	The diameter of each spherical MBA-bead was approximately 3 mm (wet form) and 2 mm in (dried form).					
37244306	4	22	theme	MBA-bead	470:477	arg1	diameter					443:450	The diameter	439:450	The diameter of each spherical MBA-bead	439:477	The diameter of each spherical MBA-bead was approximately 3 mm (wet form) and 2 mm in (dried form).					
37244306	11	23	theme	$	1159:1159	arg1	/kg					1160:1162	0.091 US$/kg	1151:1162	0.091 US$/kg	1151:1162	The cost was estimated for producing PS-biochar (0.091 US$/kg), magnetic-biochar (0.303-0.892 US$/kg), and MBA-bead (1.369-3.865 US$/kg).					
37244306	11	23	theme	$	1159:1159	arg1	PS-biochar					1139:1148	PS-biochar	1139:1148	PS-biochar (0.091 US$/kg)	1139:1163	The cost was estimated for producing PS-biochar (0.091 US$/kg), magnetic-biochar (0.303-0.892 US$/kg), and MBA-bead (1.369-3.865 US$/kg).					
37244306	9	24	theme	ion	985:987	arg1	exchange					989:996	ion exchange	985:996	ion exchange	985:996	Primary adsorption mechanisms were complexation, ion exchange, and Van der Waals force.					
37244306	2	25	theme	cross-linking	362:374	arg1	methods					376:382	physical cross-linking methods	353:382	physical cross-linking methods	353:382	MBA-bead was synthesized by physical cross-linking methods.					
37244306	0	26	theme	Easy	0:3	arg1	bead					68:71	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead	0:71	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead as super-adsorbent for adsorbing copper ions in water media.	0:132	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead as super-adsorbent for adsorbing copper ions in water media.					
37244306	11	27	dep	1.369-3.865	1219:1229	arg1	/kg					1234:1236	US$/kg	1231:1236	1.369-3.865 US$/kg	1219:1236	The cost was estimated for producing PS-biochar (0.091 US$/kg), magnetic-biochar (0.303-0.892 US$/kg), and MBA-bead (1.369-3.865 US$/kg).					
37244306	7	28	theme	adsorption	765:774	arg1	capacity					776:783	Its Langmuir maximum adsorption capacity	744:783	Its Langmuir maximum adsorption capacity for Cu2+	744:792	Its Langmuir maximum adsorption capacity for Cu2+ was 234.1 mg/g (30 °C and pHeq 5.0).					
37244306	3	29	theme	∼90	427:429	arg1	%					430:430	%	430:430	%	430:430	Results indicated that MBA-bead contained ∼90% water.					
37244306	0	30	theme	floatable	16:24	arg1	bead					68:71	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead	0:71	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead as super-adsorbent for adsorbing copper ions in water media.	0:132	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead as super-adsorbent for adsorbing copper ions in water media.					
37244306	10	31	theme	several	1053:1059	arg1	cycles					1061:1066	several cycles	1053:1066	several cycles	1053:1066	Laden MBA-bead can be reused several cycles after desorbing with NaOH or HCl.					
37244306	0	32	theme	water	121:125	arg1	media					127:131	water media	121:131	water media	121:131	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead as super-adsorbent for adsorbing copper ions in water media.					
37244306	5	33	from	77K	656:658	arg1	adsorption					642:651	nitrogen adsorption	633:651	nitrogen adsorption at 77K	633:658	Its specific surface area (262.4 m2/g) and total pore volume (0.751 cm3/g) were obtained from nitrogen adsorption at 77K.					
37244306	7	34	theme	maximum	757:763	arg1	adsorption					765:774	Its Langmuir maximum adsorption	744:774	Its Langmuir maximum adsorption capacity for Cu2+	744:792	Its Langmuir maximum adsorption capacity for Cu2+ was 234.1 mg/g (30 °C and pHeq 5.0).					
37244306	7	35	theme	Langmuir	748:755	arg1	adsorption					765:774	Its Langmuir maximum adsorption	744:774	Its Langmuir maximum adsorption capacity for Cu2+	744:792	Its Langmuir maximum adsorption capacity for Cu2+ was 234.1 mg/g (30 °C and pHeq 5.0).					
37244306	9	36	theme	adsorption	944:953	arg1	mechanisms					955:964	Primary adsorption mechanisms	936:964	Primary adsorption mechanisms	936:964	Primary adsorption mechanisms were complexation, ion exchange, and Van der Waals force.					
37244306	2	37	theme	physical	353:360	arg1	methods					376:382	physical cross-linking methods	353:382	physical cross-linking methods	353:382	MBA-bead was synthesized by physical cross-linking methods.					
37244306	1	38	theme	magnetic-biochar	208:223	arg1	properties					194:203	properties	194:203	properties of magnetic-biochar (derived from peanut shells) and hydrogel bead (MBA-bead)	194:281	This work aimed to develop innovative material by combining properties of magnetic-biochar (derived from peanut shells) and hydrogel bead (MBA-bead) and apply it for adsorbing Cu2+ in water.					
37244306	3	39	theme	%	430:430	arg1	water					432:436	∼90% water	427:436	∼90% water	427:436	Results indicated that MBA-bead contained ∼90% water.					
37244306	8	40	theme	adsorption	876:885	arg1	enthalpy					854:861	standard enthalpy	845:861	standard enthalpy (ΔH°) of the adsorption	845:885	The change in standard enthalpy (ΔH°) of the adsorption was 44.30 kJ/mol (dominant physical adsorption).					
37244306	8	40	theme	adsorption	876:885	arg1	ΔH°					864:866	ΔH°	864:866	ΔH°	864:866	The change in standard enthalpy (ΔH°) of the adsorption was 44.30 kJ/mol (dominant physical adsorption).					
37244306	5	41	theme	nitrogen	633:640	arg1	adsorption					642:651	nitrogen adsorption	633:651	nitrogen adsorption at 77K	633:658	Its specific surface area (262.4 m2/g) and total pore volume (0.751 cm3/g) were obtained from nitrogen adsorption at 77K.					
37244306	4	42	dep	form	532:535	arg1	in					522:523	in	522:523	in	522:523	The diameter of each spherical MBA-bead was approximately 3 mm (wet form) and 2 mm in (dried form).					
37244306	1	43	attach	derived	226:232	arg2	magnetic-biochar					208:223	magnetic-biochar	208:223	magnetic-biochar (derived from peanut shells)	208:252	This work aimed to develop innovative material by combining properties of magnetic-biochar (derived from peanut shells) and hydrogel bead (MBA-bead) and apply it for adsorbing Cu2+ in water.					
37244306	1	43	attach	derived	226:232	arg1	shells					246:251	peanut shells	239:251	peanut shells	239:251	This work aimed to develop innovative material by combining properties of magnetic-biochar (derived from peanut shells) and hydrogel bead (MBA-bead) and apply it for adsorbing Cu2+ in water.					
37244306	9	44	theme	der	1007:1009	arg1	force					1017:1021	Van der Waals force	1003:1021	Van der Waals force	1003:1021	Primary adsorption mechanisms were complexation, ion exchange, and Van der Waals force.					
37244306	0	45	theme	magnetic-biochar/alginate	42:66	arg1	bead					68:71	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead	0:71	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead as super-adsorbent for adsorbing copper ions in water media.	0:132	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead as super-adsorbent for adsorbing copper ions in water media.					
37244306	4	46	theme	wet	503:505	arg1	mm					499:500	approximately 3 mm	483:500	approximately 3 mm (wet form)	483:511	The diameter of each spherical MBA-bead was approximately 3 mm (wet form) and 2 mm in (dried form).					
37244306	4	46	theme	wet	503:505	arg1	form					507:510	wet form	503:510	wet form	503:510	The diameter of each spherical MBA-bead was approximately 3 mm (wet form) and 2 mm in (dried form).					
37244306	9	47	theme	Waals	1011:1015	arg1	force					1017:1021	Van der Waals force	1003:1021	Van der Waals force	1003:1021	Primary adsorption mechanisms were complexation, ion exchange, and Van der Waals force.					
37244306	0	48	theme	recyclable	31:40	arg1	bead					68:71	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead	0:71	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead as super-adsorbent for adsorbing copper ions in water media.	0:132	Easy separable, floatable, and recyclable magnetic-biochar/alginate bead as super-adsorbent for adsorbing copper ions in water media.					
37244306	6	49	theme	diffraction	667:677	arg1	data					679:682	X-ray diffraction data	661:682	X-ray diffraction data	661:682	X-ray diffraction data confirmed Fe3O4 presented in magnetic-biochar and MBA-bead.					
37244306	10	50	theme	Laden	1024:1028	arg1	MBA-bead					1030:1037	Laden MBA-bead	1024:1037	Laden MBA-bead	1024:1037	Laden MBA-bead can be reused several cycles after desorbing with NaOH or HCl.					
37244306	11	51	dep	/kg	1234:1236	arg1	US					1231:1232	US	1231:1232	US	1231:1232	The cost was estimated for producing PS-biochar (0.091 US$/kg), magnetic-biochar (0.303-0.892 US$/kg), and MBA-bead (1.369-3.865 US$/kg).					
37244306	3	52	contain	contained	417:425	arg2	water					432:436	∼90% water	427:436	∼90% water	427:436	Results indicated that MBA-bead contained ∼90% water.					
37244306	3	52	contain	contained	417:425	arg1	MBA-bead					408:415	MBA-bead	408:415	MBA-bead	408:415	Results indicated that MBA-bead contained ∼90% water.					
37244306	6	53	theme	X-ray	661:665	arg1	data					679:682	X-ray diffraction data	661:682	X-ray diffraction data	661:682	X-ray diffraction data confirmed Fe3O4 presented in magnetic-biochar and MBA-bead.					
37244306	8	54	theme	standard	845:852	arg1	enthalpy					854:861	standard enthalpy	845:861	standard enthalpy (ΔH°) of the adsorption	845:885	The change in standard enthalpy (ΔH°) of the adsorption was 44.30 kJ/mol (dominant physical adsorption).					
37244306	8	54	theme	standard	845:852	arg1	ΔH°					864:866	ΔH°	864:866	ΔH°	864:866	The change in standard enthalpy (ΔH°) of the adsorption was 44.30 kJ/mol (dominant physical adsorption).					
37244306	1	55	theme	peanut	239:244	arg1	shells					246:251	peanut shells	239:251	peanut shells	239:251	This work aimed to develop innovative material by combining properties of magnetic-biochar (derived from peanut shells) and hydrogel bead (MBA-bead) and apply it for adsorbing Cu2+ in water.					
37244306	1	56	from	Cu2+	310:313	arg1	water					318:322	water	318:322	water	318:322	This work aimed to develop innovative material by combining properties of magnetic-biochar (derived from peanut shells) and hydrogel bead (MBA-bead) and apply it for adsorbing Cu2+ in water.					
35216525	5	0	theme	tissue	821:826	arg1	specimens					828:836	the native tissue specimens	810:836	the native tissue specimens in cyclic compression-tension	810:866	Through large-strain mechanical measurements, the hydrogels' properties were compared to human brain tissue and the samples containing ADA revealed similar mechanical properties to the native tissue specimens in cyclic compression-tension.					
35216525	6	1	theme	fibroblasts	957:967	arg1	viability					915:923	a high viability	908:923	a high viability of encapsulated mouse embryonic fibroblasts	908:967	In vitro characterization demonstrated a high viability of encapsulated mouse embryonic fibroblasts and a spreading of the cells in case of ADA-free samples.					
35216525	6	1	theme	fibroblasts	957:967	arg1	spreading					975:983	a spreading	973:983	a spreading of the cells in case of ADA-free samples	973:1024	In vitro characterization demonstrated a high viability of encapsulated mouse embryonic fibroblasts and a spreading of the cells in case of ADA-free samples.					
35216525	7	2	theme	surgical	1199:1206	arg1	training					1208:1215	surgical training	1199:1215	surgical training	1199:1215	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	5	3	theme	brain	724:728	arg1	tissue					730:735	human brain tissue	718:735	human brain tissue	718:735	Through large-strain mechanical measurements, the hydrogels' properties were compared to human brain tissue and the samples containing ADA revealed similar mechanical properties to the native tissue specimens in cyclic compression-tension.					
35216525	6	4	theme	cells	992:996	arg1	viability					915:923	a high viability	908:923	a high viability of encapsulated mouse embryonic fibroblasts	908:967	In vitro characterization demonstrated a high viability of encapsulated mouse embryonic fibroblasts and a spreading of the cells in case of ADA-free samples.					
35216525	6	4	theme	cells	992:996	arg1	spreading					975:983	a spreading	973:983	a spreading of the cells in case of ADA-free samples	973:1024	In vitro characterization demonstrated a high viability of encapsulated mouse embryonic fibroblasts and a spreading of the cells in case of ADA-free samples.					
35216525	6	5	theme	high	910:913	arg1	viability					915:923	a high viability	908:923	a high viability of encapsulated mouse embryonic fibroblasts	908:967	In vitro characterization demonstrated a high viability of encapsulated mouse embryonic fibroblasts and a spreading of the cells in case of ADA-free samples.					
35216525	5	6	theme	large-strain	637:648	arg1	measurements					661:672	large-strain mechanical measurements	637:672	large-strain mechanical measurements	637:672	Through large-strain mechanical measurements, the hydrogels' properties were compared to human brain tissue and the samples containing ADA revealed similar mechanical properties to the native tissue specimens in cyclic compression-tension.					
35216525	6	7	theme	encapsulated	928:939	arg1	fibroblasts					957:967	encapsulated mouse embryonic fibroblasts	928:967	encapsulated mouse embryonic fibroblasts	928:967	In vitro characterization demonstrated a high viability of encapsulated mouse embryonic fibroblasts and a spreading of the cells in case of ADA-free samples.					
35216525	4	8	theme	crosslinking	609:620	arg1	times					622:626	crosslinking times	609:626	crosslinking times	609:626	The stability under incubation conditions was improved by adjusting crosslinking times.					
35216525	5	9	theme	similar	777:783	arg1	properties					796:805	similar mechanical properties	777:805	similar mechanical properties	777:805	Through large-strain mechanical measurements, the hydrogels' properties were compared to human brain tissue and the samples containing ADA revealed similar mechanical properties to the native tissue specimens in cyclic compression-tension.					
35216525	7	10	theme	techniques	1187:1196	arg1	engineering					1225:1235	tissue engineering	1218:1235	tissue engineering	1218:1235	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	10	theme	techniques	1187:1196	arg1	modeling					1242:1249	modeling	1242:1249	modeling of the mechanical behavior of tissues	1242:1287	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	10	theme	techniques	1187:1196	arg1	testing					1160:1166	testing	1160:1166	testing of medical imaging techniques	1160:1196	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	10	theme	techniques	1187:1196	arg1	gear					1154:1157	safety gear	1147:1157	safety gear	1147:1157	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	10	theme	techniques	1187:1196	arg1	training					1208:1215	surgical training	1199:1215	surgical training	1199:1215	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	5	11	theme	mechanical	650:659	arg1	measurements					661:672	large-strain mechanical measurements	637:672	large-strain mechanical measurements	637:672	Through large-strain mechanical measurements, the hydrogels' properties were compared to human brain tissue and the samples containing ADA revealed similar mechanical properties to the native tissue specimens in cyclic compression-tension.					
35216525	9	12	theme	different	1506:1514	arg1	methods					1516:1522	different methods	1506:1522	different methods	1506:1522	In this article, different methods are used to evaluate a novel hydrogel material and its suitability as brain mimicking matrix.					
35216525	5	13	theme	mechanical	785:794	arg1	properties					796:805	similar mechanical properties	777:805	similar mechanical properties	777:805	Through large-strain mechanical measurements, the hydrogels' properties were compared to human brain tissue and the samples containing ADA revealed similar mechanical properties to the native tissue specimens in cyclic compression-tension.					
35216525	2	14	theme	Hyaluronic	172:181	arg1	acid					183:186	Hyaluronic acid	172:186	Hyaluronic acid	172:186	Hyaluronic acid is a linear polysaccharide native to the human extracellular matrix.					
35216525	2	14	theme	Hyaluronic	172:181	arg1	polysaccharide					200:213	a linear polysaccharide	191:213	a linear polysaccharide native to the human extracellular matrix	191:254	Hyaluronic acid is a linear polysaccharide native to the human extracellular matrix.					
35216525	5	15	theme	human	718:722	arg1	tissue					730:735	human brain tissue	718:735	human brain tissue	718:735	Through large-strain mechanical measurements, the hydrogels' properties were compared to human brain tissue and the samples containing ADA revealed similar mechanical properties to the native tissue specimens in cyclic compression-tension.					
35216525	7	16	theme	Impact	1027:1032	arg1	materials					1060:1068	Impact statement Brain mimicking materials	1027:1068	Impact statement Brain mimicking materials	1027:1068	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	17	theme	Brain	1044:1048	arg1	materials					1060:1068	Impact statement Brain mimicking materials	1027:1068	Impact statement Brain mimicking materials	1027:1068	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	9	18	theme	novel	1547:1551	arg1	material					1562:1569	a novel hydrogel material	1545:1569	a novel hydrogel material	1545:1569	In this article, different methods are used to evaluate a novel hydrogel material and its suitability as brain mimicking matrix.					
35216525	7	19	theme	mechanical	1258:1267	arg1	behavior					1269:1276	the mechanical behavior	1254:1276	the mechanical behavior of tissues	1254:1287	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	20	theme	medical	1171:1177	arg1	techniques					1187:1196	medical imaging techniques	1171:1196	medical imaging techniques	1171:1196	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	6	21	theme	In	869:870	arg1	characterization					878:893	In vitro characterization	869:893	In vitro characterization	869:893	In vitro characterization demonstrated a high viability of encapsulated mouse embryonic fibroblasts and a spreading of the cells in case of ADA-free samples.					
35216525	7	22	theme	tissue	1218:1223	arg1	engineering					1225:1235	tissue engineering	1218:1235	tissue engineering	1218:1235	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	23	theme	imaging	1179:1185	arg1	techniques					1187:1196	medical imaging techniques	1171:1196	medical imaging techniques	1171:1196	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	24	theme	mimicking	1050:1058	arg1	materials					1060:1068	Impact statement Brain mimicking materials	1027:1068	Impact statement Brain mimicking materials	1027:1068	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	0	25	theme	Hyaluronic	9:18	arg1	Hydrogels					39:47	Oxidized Hyaluronic Acid-Gelatin-Based Hydrogels	0:47	Oxidized Hyaluronic Acid-Gelatin-Based Hydrogels for Tissue Engineering and Soft Tissue	0:86	Oxidized Hyaluronic Acid-Gelatin-Based Hydrogels for Tissue Engineering and Soft Tissue Mimicking.					
35216525	7	26	theme	testing	1160:1166	arg1	development					1132:1142	the development	1128:1142	the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues	1128:1287	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	9	27	theme	hydrogel	1553:1560	arg1	material					1562:1569	a novel hydrogel material	1545:1569	a novel hydrogel material	1545:1569	In this article, different methods are used to evaluate a novel hydrogel material and its suitability as brain mimicking matrix.					
35216525	8	28	dep	suit	1458:1461	arg1	applications					1475:1486	the various applications	1463:1486	the various applications	1463:1486	The materials must resemble the microstructure, chemistry, and mechanical properties of the native tissue extracellular matrix while being adjustable in degradation to suit the various applications.					
35216525	0	29	theme	Oxidized	0:7	arg1	Hydrogels					39:47	Oxidized Hyaluronic Acid-Gelatin-Based Hydrogels	0:47	Oxidized Hyaluronic Acid-Gelatin-Based Hydrogels for Tissue Engineering and Soft Tissue	0:86	Oxidized Hyaluronic Acid-Gelatin-Based Hydrogels for Tissue Engineering and Soft Tissue Mimicking.					
35216525	3	30	with	tissue	512:517	arg1	applications					468:479	potential applications	458:479	potential applications in soft tissue engineering	458:506	In this study, we first develop and characterize two hydrogel compositions built from oxidized HA and gelatin with and without alginate-di-aldehyde (ADA) crosslinked by ionic and enzymatic agents with potential applications in soft tissue engineering and tissue mimicking structures.					
35216525	8	31	dep	microstructure	1322:1335	arg1	the					1318:1320	the	1318:1320	the	1318:1320	The materials must resemble the microstructure, chemistry, and mechanical properties of the native tissue extracellular matrix while being adjustable in degradation to suit the various applications.					
35216525	3	32	theme	enzymatic	436:444	arg1	agents					446:451	ionic and enzymatic agents	426:451	ionic and enzymatic agents with potential applications in soft tissue engineering	426:506	In this study, we first develop and characterize two hydrogel compositions built from oxidized HA and gelatin with and without alginate-di-aldehyde (ADA) crosslinked by ionic and enzymatic agents with potential applications in soft tissue engineering and tissue mimicking structures.					
35216525	7	33	theme	engineering	1225:1235	arg1	development					1132:1142	the development	1128:1142	the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues	1128:1287	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	34	theme	gear	1154:1157	arg1	development					1132:1142	the development	1128:1142	the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues	1128:1287	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	35	theme	statement	1034:1042	arg1	materials					1060:1068	Impact statement Brain mimicking materials	1027:1068	Impact statement Brain mimicking materials	1027:1068	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	1	36	theme	ideal	113:117	arg1	Hydrogels					99:107	Hydrogels	99:107	Hydrogels	99:107	Hydrogels are ideal materials for mimicking and engineering soft tissue.					
35216525	1	36	theme	ideal	113:117	arg1	materials					119:127	ideal materials	113:127	ideal materials for mimicking and engineering soft tissue	113:169	Hydrogels are ideal materials for mimicking and engineering soft tissue.					
35216525	6	37	dep	In	869:870	arg1	vitro					872:876	vitro	872:876	vitro	872:876	In vitro characterization demonstrated a high viability of encapsulated mouse embryonic fibroblasts and a spreading of the cells in case of ADA-free samples.					
35216525	7	38	theme	industrial	1106:1115	arg1	fields					1117:1122	several medical and industrial fields	1086:1122	several medical and industrial fields	1086:1122	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	0	39	theme	Acid-Gelatin-Based	20:37	arg1	Hydrogels					39:47	Oxidized Hyaluronic Acid-Gelatin-Based Hydrogels	0:47	Oxidized Hyaluronic Acid-Gelatin-Based Hydrogels for Tissue Engineering and Soft Tissue	0:86	Oxidized Hyaluronic Acid-Gelatin-Based Hydrogels for Tissue Engineering and Soft Tissue Mimicking.					
35216525	5	40	contain	containing	753:762	arg2	ADA					764:766	ADA	764:766	ADA	764:766	Through large-strain mechanical measurements, the hydrogels' properties were compared to human brain tissue and the samples containing ADA revealed similar mechanical properties to the native tissue specimens in cyclic compression-tension.					
35216525	5	40	contain	containing	753:762	arg1	samples					745:751	the samples	741:751	the samples containing ADA	741:766	Through large-strain mechanical measurements, the hydrogels' properties were compared to human brain tissue and the samples containing ADA revealed similar mechanical properties to the native tissue specimens in cyclic compression-tension.					
35216525	2	41	theme	linear	193:198	arg1	acid					183:186	Hyaluronic acid	172:186	Hyaluronic acid	172:186	Hyaluronic acid is a linear polysaccharide native to the human extracellular matrix.					
35216525	2	41	theme	linear	193:198	arg1	polysaccharide					200:213	a linear polysaccharide	191:213	a linear polysaccharide native to the human extracellular matrix	191:254	Hyaluronic acid is a linear polysaccharide native to the human extracellular matrix.					
35216525	8	42	theme	matrix	1410:1415	arg1	microstructure					1322:1335	microstructure	1322:1335	microstructure	1322:1335	The materials must resemble the microstructure, chemistry, and mechanical properties of the native tissue extracellular matrix while being adjustable in degradation to suit the various applications.					
35216525	8	42	theme	matrix	1410:1415	arg1	properties					1364:1373	mechanical properties	1353:1373	mechanical properties	1353:1373	The materials must resemble the microstructure, chemistry, and mechanical properties of the native tissue extracellular matrix while being adjustable in degradation to suit the various applications.					
35216525	8	42	theme	matrix	1410:1415	arg1	chemistry					1338:1346	chemistry	1338:1346	chemistry	1338:1346	The materials must resemble the microstructure, chemistry, and mechanical properties of the native tissue extracellular matrix while being adjustable in degradation to suit the various applications.					
35216525	9	43	used	used	1528:1531	arg2	methods					1516:1522	different methods	1506:1522	different methods	1506:1522	In this article, different methods are used to evaluate a novel hydrogel material and its suitability as brain mimicking matrix.					
35216525	3	44	theme	soft	484:487	arg1	engineering					496:506	soft tissue engineering	484:506	soft tissue engineering	484:506	In this study, we first develop and characterize two hydrogel compositions built from oxidized HA and gelatin with and without alginate-di-aldehyde (ADA) crosslinked by ionic and enzymatic agents with potential applications in soft tissue engineering and tissue mimicking structures.					
35216525	0	45	theme	Tissue	53:58	arg1	Engineering					60:70	Tissue Engineering	53:70	Tissue Engineering	53:70	Oxidized Hyaluronic Acid-Gelatin-Based Hydrogels for Tissue Engineering and Soft Tissue Mimicking.					
35216525	6	46	theme	embryonic	947:955	arg1	fibroblasts					957:967	encapsulated mouse embryonic fibroblasts	928:967	encapsulated mouse embryonic fibroblasts	928:967	In vitro characterization demonstrated a high viability of encapsulated mouse embryonic fibroblasts and a spreading of the cells in case of ADA-free samples.					
35216525	3	47	theme	potential	458:466	arg1	applications					468:479	potential applications	458:479	potential applications in soft tissue engineering	458:506	In this study, we first develop and characterize two hydrogel compositions built from oxidized HA and gelatin with and without alginate-di-aldehyde (ADA) crosslinked by ionic and enzymatic agents with potential applications in soft tissue engineering and tissue mimicking structures.					
35216525	3	48	theme	ionic	426:430	arg1	agents					446:451	ionic and enzymatic agents	426:451	ionic and enzymatic agents with potential applications in soft tissue engineering	426:506	In this study, we first develop and characterize two hydrogel compositions built from oxidized HA and gelatin with and without alginate-di-aldehyde (ADA) crosslinked by ionic and enzymatic agents with potential applications in soft tissue engineering and tissue mimicking structures.					
35216525	8	49	theme	mechanical	1353:1362	arg1	properties					1364:1373	mechanical properties	1353:1373	mechanical properties	1353:1373	The materials must resemble the microstructure, chemistry, and mechanical properties of the native tissue extracellular matrix while being adjustable in degradation to suit the various applications.					
35216525	5	50	theme	cyclic	841:846	arg1	compression-tension					848:866	cyclic compression-tension	841:866	cyclic compression-tension	841:866	Through large-strain mechanical measurements, the hydrogels' properties were compared to human brain tissue and the samples containing ADA revealed similar mechanical properties to the native tissue specimens in cyclic compression-tension.					
35216525	6	51	theme	mouse	941:945	arg1	fibroblasts					957:967	encapsulated mouse embryonic fibroblasts	928:967	encapsulated mouse embryonic fibroblasts	928:967	In vitro characterization demonstrated a high viability of encapsulated mouse embryonic fibroblasts and a spreading of the cells in case of ADA-free samples.					
35216525	7	52	theme	behavior	1269:1276	arg1	engineering					1225:1235	tissue engineering	1218:1235	tissue engineering	1218:1235	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	52	theme	behavior	1269:1276	arg1	modeling					1242:1249	modeling	1242:1249	modeling of the mechanical behavior of tissues	1242:1287	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	52	theme	behavior	1269:1276	arg1	testing					1160:1166	testing	1160:1166	testing of medical imaging techniques	1160:1196	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	52	theme	behavior	1269:1276	arg1	gear					1154:1157	safety gear	1147:1157	safety gear	1147:1157	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	52	theme	behavior	1269:1276	arg1	training					1208:1215	surgical training	1199:1215	surgical training	1199:1215	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	7	53	theme	training	1208:1215	arg1	development					1132:1142	the development	1128:1142	the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues	1128:1287	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	3	54	theme	hydrogel	310:317	arg1	compositions					319:330	two hydrogel compositions	306:330	two hydrogel compositions built from oxidized HA and gelatin with and without alginate-di-aldehyde (ADA) crosslinked by ionic and enzymatic agents with potential applications in soft tissue engineering and tissue mimicking structures	306:538	In this study, we first develop and characterize two hydrogel compositions built from oxidized HA and gelatin with and without alginate-di-aldehyde (ADA) crosslinked by ionic and enzymatic agents with potential applications in soft tissue engineering and tissue mimicking structures.					
35216525	5	55	theme	native	814:819	arg1	specimens					828:836	the native tissue specimens	810:836	the native tissue specimens in cyclic compression-tension	810:866	Through large-strain mechanical measurements, the hydrogels' properties were compared to human brain tissue and the samples containing ADA revealed similar mechanical properties to the native tissue specimens in cyclic compression-tension.					
35216525	2	56	theme	extracellular	235:247	arg1	matrix					249:254	the human extracellular matrix	225:254	the human extracellular matrix	225:254	Hyaluronic acid is a linear polysaccharide native to the human extracellular matrix.					
35216525	3	57	from	applications	468:479	arg1	engineering					496:506	soft tissue engineering	484:506	soft tissue engineering	484:506	In this study, we first develop and characterize two hydrogel compositions built from oxidized HA and gelatin with and without alginate-di-aldehyde (ADA) crosslinked by ionic and enzymatic agents with potential applications in soft tissue engineering and tissue mimicking structures.					
35216525	7	58	theme	safety	1147:1152	arg1	gear					1154:1157	safety gear	1147:1157	safety gear	1147:1157	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	6	59	theme	ADA-free	1009:1016	arg1	samples					1018:1024	ADA-free samples	1009:1024	ADA-free samples	1009:1024	In vitro characterization demonstrated a high viability of encapsulated mouse embryonic fibroblasts and a spreading of the cells in case of ADA-free samples.					
35216525	2	60	theme	human	229:233	arg1	matrix					249:254	the human extracellular matrix	225:254	the human extracellular matrix	225:254	Hyaluronic acid is a linear polysaccharide native to the human extracellular matrix.					
35216525	7	61	theme	several	1086:1092	arg1	fields					1117:1122	several medical and industrial fields	1086:1122	several medical and industrial fields	1086:1122	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	8	62	from	degradation	1443:1453	arg1	adjustable					1429:1438	adjustable	1429:1438	adjustable	1429:1438	The materials must resemble the microstructure, chemistry, and mechanical properties of the native tissue extracellular matrix while being adjustable in degradation to suit the various applications.					
35216525	4	63	theme	incubation	561:570	arg1	conditions					572:581	incubation conditions	561:581	incubation conditions	561:581	The stability under incubation conditions was improved by adjusting crosslinking times.					
35216525	3	64	with	agents	446:451	arg1	applications					468:479	potential applications	458:479	potential applications in soft tissue engineering	458:506	In this study, we first develop and characterize two hydrogel compositions built from oxidized HA and gelatin with and without alginate-di-aldehyde (ADA) crosslinked by ionic and enzymatic agents with potential applications in soft tissue engineering and tissue mimicking structures.					
35216525	5	65	from	specimens	828:836	arg1	compression-tension					848:866	cyclic compression-tension	841:866	cyclic compression-tension	841:866	Through large-strain mechanical measurements, the hydrogels' properties were compared to human brain tissue and the samples containing ADA revealed similar mechanical properties to the native tissue specimens in cyclic compression-tension.					
35216525	7	66	theme	medical	1094:1100	arg1	fields					1117:1122	several medical and industrial fields	1086:1122	several medical and industrial fields	1086:1122	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	3	67	theme	tissue	489:494	arg1	engineering					496:506	soft tissue engineering	484:506	soft tissue engineering	484:506	In this study, we first develop and characterize two hydrogel compositions built from oxidized HA and gelatin with and without alginate-di-aldehyde (ADA) crosslinked by ionic and enzymatic agents with potential applications in soft tissue engineering and tissue mimicking structures.					
35216525	8	68	theme	various	1467:1473	arg1	applications					1475:1486	the various applications	1463:1486	the various applications	1463:1486	The materials must resemble the microstructure, chemistry, and mechanical properties of the native tissue extracellular matrix while being adjustable in degradation to suit the various applications.					
35216525	2	69	theme	native	215:220	arg1	acid					183:186	Hyaluronic acid	172:186	Hyaluronic acid	172:186	Hyaluronic acid is a linear polysaccharide native to the human extracellular matrix.					
35216525	2	69	theme	native	215:220	arg1	polysaccharide					200:213	a linear polysaccharide	191:213	a linear polysaccharide native to the human extracellular matrix	191:254	Hyaluronic acid is a linear polysaccharide native to the human extracellular matrix.					
35216525	8	70	theme	extracellular	1396:1408	arg1	matrix					1410:1415	the native tissue extracellular matrix	1378:1415	the native tissue extracellular matrix	1378:1415	The materials must resemble the microstructure, chemistry, and mechanical properties of the native tissue extracellular matrix while being adjustable in degradation to suit the various applications.					
35216525	8	71	from	adjustable	1429:1438	arg1	degradation					1443:1453	degradation	1443:1453	degradation to suit the various applications	1443:1486	The materials must resemble the microstructure, chemistry, and mechanical properties of the native tissue extracellular matrix while being adjustable in degradation to suit the various applications.					
35216525	8	72	theme	native	1382:1387	arg1	matrix					1410:1415	the native tissue extracellular matrix	1378:1415	the native tissue extracellular matrix	1378:1415	The materials must resemble the microstructure, chemistry, and mechanical properties of the native tissue extracellular matrix while being adjustable in degradation to suit the various applications.					
35216525	3	73	theme	oxidized	343:350	arg1	HA					352:353	HA	352:353	HA	352:353	In this study, we first develop and characterize two hydrogel compositions built from oxidized HA and gelatin with and without alginate-di-aldehyde (ADA) crosslinked by ionic and enzymatic agents with potential applications in soft tissue engineering and tissue mimicking structures.					
35216525	1	74	theme	soft	159:162	arg1	tissue					164:169	soft tissue	159:169	soft tissue	159:169	Hydrogels are ideal materials for mimicking and engineering soft tissue.					
35216525	0	75	theme	Soft	76:79	arg1	Tissue					81:86	Soft Tissue	76:86	Soft Tissue	76:86	Oxidized Hyaluronic Acid-Gelatin-Based Hydrogels for Tissue Engineering and Soft Tissue Mimicking.					
35216525	7	76	theme	modeling	1242:1249	arg1	development					1132:1142	the development	1128:1142	the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues	1128:1287	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
35216525	8	77	theme	tissue	1389:1394	arg1	matrix					1410:1415	the native tissue extracellular matrix	1378:1415	the native tissue extracellular matrix	1378:1415	The materials must resemble the microstructure, chemistry, and mechanical properties of the native tissue extracellular matrix while being adjustable in degradation to suit the various applications.					
35216525	7	78	theme	tissues	1281:1287	arg1	behavior					1269:1276	the mechanical behavior	1254:1276	the mechanical behavior of tissues	1254:1287	Impact statement Brain mimicking materials are required in several medical and industrial fields for the development of safety gear, testing of medical imaging techniques, surgical training, tissue engineering, and modeling of the mechanical behavior of tissues.					
36563810	9	0	theme	hybrid	1654:1659	arg1	complicity					1671:1680	the hybrid materials complicity	1650:1680	the hybrid materials complicity	1650:1680	The present work shows that pseudo first order could describe the MB adsorption onto AG while it couldn't describe the MB adsorption onto the functionalized sorbents due to the hybrid materials complicity.					
36563810	7	1	theme	strong	1277:1282	arg1	interactions					1298:1309	strong electrostatic interactions	1277:1309	strong electrostatic interactions	1277:1309	The highest adsorption capacities were observed at pH ~ 5, meanwhile the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions.					
36563810	1	2	theme	sorbent	170:176	arg1	AG-GO-HMTA					289:298	AG-GO-HMTA	289:298	AG-GO-HMTA	289:298	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	1	2	theme	sorbent	170:176	arg1	composite					278:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite	149:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA)	149:299	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	1	3	theme	oxide	267:271	arg1	AG-GO-HMTA					289:298	AG-GO-HMTA	289:298	AG-GO-HMTA	289:298	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	1	3	theme	oxide	267:271	arg1	composite					278:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite	149:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA)	149:299	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	9	4	theme	MB	1596:1597	arg1	adsorption					1599:1608	the MB adsorption	1592:1608	the MB adsorption onto the functionalized sorbents due to the hybrid materials complicity	1592:1680	The present work shows that pseudo first order could describe the MB adsorption onto AG while it couldn't describe the MB adsorption onto the functionalized sorbents due to the hybrid materials complicity.					
36563810	2	5	from	concentration	461:473	arg1	removal					535:541	methylene blue (MB) removal	515:541	methylene blue (MB) removal from water	515:552	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	1	6	dep	oxide	267:271	arg1	GO					274:275	GO	274:275	GO	274:275	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	6	7	theme	high	1059:1062	arg1	dispersion					1072:1081	high quality dispersion	1059:1081	high quality dispersion of HMTA within the AG-GO matrix	1059:1113	AG-GO HMTA displays a well-defined porous structure and this desired morphology arising from high quality dispersion of HMTA within the AG-GO matrix.					
36563810	7	8	theme	adsorption	1128:1137	arg1	capacities					1139:1148	The highest adsorption capacities	1116:1148	The highest adsorption capacities	1116:1148	The highest adsorption capacities were observed at pH ~ 5, meanwhile the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions.					
36563810	3	9	dep	thermal	656:662	arg1	DTG					675:677	DTG	675:677	DTG	675:677	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	3	9	dep	thermal	656:662	arg1	TGA					670:672	TGA	670:672	TGA	670:672	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	0	10	theme	aqueous	116:122	arg1	solution					124:131	aqueous solution	116:131	aqueous solution	116:131	Hexamethylenetetramine functionalized graphene oxide-alginate beads nanocomposite as efficient sorbent for dye from aqueous solution.					
36563810	2	11	from	dosage	476:481	arg1	removal					535:541	methylene blue (MB) removal	515:541	methylene blue (MB) removal from water	515:552	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	6	12	theme	quality	1064:1070	arg1	dispersion					1072:1081	high quality dispersion	1059:1081	high quality dispersion of HMTA within the AG-GO matrix	1059:1113	AG-GO HMTA displays a well-defined porous structure and this desired morphology arising from high quality dispersion of HMTA within the AG-GO matrix.					
36563810	9	13	theme	materials	1661:1669	arg1	complicity					1671:1680	the hybrid materials complicity	1650:1680	the hybrid materials complicity	1650:1680	The present work shows that pseudo first order could describe the MB adsorption onto AG while it couldn't describe the MB adsorption onto the functionalized sorbents due to the hybrid materials complicity.					
36563810	3	14	theme	morphological	685:697	arg1	techniques					724:733	structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques	620:733	structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques	620:733	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	4	15	theme	Langmuir	789:796	arg1	isotherms					813:821	the Langmuir and Freundlich isotherms	785:821	isotherms	813:821	The equilibrium adsorption data was described by the Langmuir and Freundlich isotherms, and the adsorption kinetic and thermodynamic parameters were investigated.					
36563810	2	16	theme	contact	435:441	arg1	time					443:446	contact time	435:446	contact time	435:446	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	2	17	theme	pH	424:425	arg1	impact					427:432	pH impact	424:432	pH impact	424:432	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	2	18	theme	blue	525:528	arg1	methylene					515:523	methylene blue	515:528	methylene blue (MB) removal from water	515:552	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	2	18	theme	blue	525:528	arg1	MB					531:532	MB	531:532	MB	531:532	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	2	19	from	selectivity	484:494	arg1	removal					535:541	methylene blue (MB) removal	515:541	methylene blue (MB) removal from water	515:552	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	4	20	theme	adsorption	752:761	arg1	data					763:766	The equilibrium adsorption data	736:766	The equilibrium adsorption data	736:766	The equilibrium adsorption data was described by the Langmuir and Freundlich isotherms, and the adsorption kinetic and thermodynamic parameters were investigated.					
36563810	7	21	located	observed	1155:1162	arg2	capacities					1139:1148	The highest adsorption capacities	1116:1148	The highest adsorption capacities	1116:1148	The highest adsorption capacities were observed at pH ~ 5, meanwhile the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions.					
36563810	7	21	located	observed	1155:1162	arg1	meanwhile					1175:1183	meanwhile	1175:1183	meanwhile	1175:1183	The highest adsorption capacities were observed at pH ~ 5, meanwhile the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions.					
36563810	7	21	located	observed	1155:1162	arg1	pH ~ 5					1167:1172	pH ~ 5	1167:1172	pH ~ 5	1167:1172	The highest adsorption capacities were observed at pH ~ 5, meanwhile the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions.					
36563810	2	22	theme	dye	457:459	arg1	concentration					461:473	initial dye concentration	449:473	initial dye concentration	449:473	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	0	23	theme	functionalized	23:36	arg1	beads					62:66	Hexamethylenetetramine functionalized graphene oxide-alginate beads	0:66	Hexamethylenetetramine functionalized graphene oxide-alginate beads	0:66	Hexamethylenetetramine functionalized graphene oxide-alginate beads nanocomposite as efficient sorbent for dye from aqueous solution.					
36563810	6	24	theme	porous	1001:1006	arg1	structure					1008:1016	a well-defined porous structure	986:1016	a well-defined porous structure	986:1016	AG-GO HMTA displays a well-defined porous structure and this desired morphology arising from high quality dispersion of HMTA within the AG-GO matrix.					
36563810	4	25	theme	Freundlich	802:811	arg1	isotherms					813:821	the Langmuir and Freundlich isotherms	785:821	isotherms	813:821	The equilibrium adsorption data was described by the Langmuir and Freundlich isotherms, and the adsorption kinetic and thermodynamic parameters were investigated.					
36563810	1	26	dep	sorbent	170:176	arg1	functionalized					209:222	functionalized	209:222	functionalized	209:222	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	9	27	theme	functionalized	1619:1632	arg1	sorbents					1634:1641	the functionalized sorbents	1615:1641	the functionalized sorbents	1615:1641	The present work shows that pseudo first order could describe the MB adsorption onto AG while it couldn't describe the MB adsorption onto the functionalized sorbents due to the hybrid materials complicity.					
36563810	0	28	theme	Hexamethylenetetramine	0:21	arg1	beads					62:66	Hexamethylenetetramine functionalized graphene oxide-alginate beads	0:66	Hexamethylenetetramine functionalized graphene oxide-alginate beads	0:66	Hexamethylenetetramine functionalized graphene oxide-alginate beads nanocomposite as efficient sorbent for dye from aqueous solution.					
36563810	9	29	theme	pseudo	1505:1510	arg1	order					1518:1522	pseudo first order	1505:1522	pseudo first order	1505:1522	The present work shows that pseudo first order could describe the MB adsorption onto AG while it couldn't describe the MB adsorption onto the functionalized sorbents due to the hybrid materials complicity.					
36563810	3	30	theme	prepared	577:584	arg1	sorbents					586:593	The prepared sorbents	573:593	The prepared sorbents	573:593	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	6	31	theme	AG-GO	966:970	arg1	HMTA					972:975	AG-GO HMTA	966:975	AG-GO HMTA	966:975	AG-GO HMTA displays a well-defined porous structure and this desired morphology arising from high quality dispersion of HMTA within the AG-GO matrix.					
36563810	2	32	theme	Adsorption	395:404	arg1	concentration					461:473	initial dye concentration	449:473	initial dye concentration	449:473	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	2	32	theme	Adsorption	395:404	arg1	reusability					500:510	reusability	500:510	reusability	500:510	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	2	32	theme	Adsorption	395:404	arg1	impact					427:432	pH impact	424:432	pH impact	424:432	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	2	32	theme	Adsorption	395:404	arg1	time					443:446	contact time	435:446	contact time	435:446	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	2	32	theme	Adsorption	395:404	arg1	dosage					476:481	dosage	476:481	dosage	476:481	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	2	32	theme	Adsorption	395:404	arg1	factors					406:412	Adsorption factors	395:412	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water	395:552	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	2	32	theme	Adsorption	395:404	arg1	selectivity					484:494	selectivity	484:494	selectivity	484:494	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	0	33	theme	oxide-alginate	47:60	arg1	beads					62:66	Hexamethylenetetramine functionalized graphene oxide-alginate beads	0:66	Hexamethylenetetramine functionalized graphene oxide-alginate beads	0:66	Hexamethylenetetramine functionalized graphene oxide-alginate beads nanocomposite as efficient sorbent for dye from aqueous solution.					
36563810	3	34	dep	TGA	670:672	arg1	e.g.					665:668	e.g.	665:668	e.g.	665:668	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	3	35	dep	structural	620:629	arg1	EDAX					649:652	EDAX	649:652	EDAX	649:652	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	3	35	dep	structural	620:629	arg1	FT-IR					642:646	FT-IR	642:646	FT-IR	642:646	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	3	35	dep	structural	620:629	arg1	XRD					637:639	XRD	637:639	XRD	637:639	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	0	36	theme	graphene	38:45	arg1	beads					62:66	Hexamethylenetetramine functionalized graphene oxide-alginate beads	0:66	Hexamethylenetetramine functionalized graphene oxide-alginate beads	0:66	Hexamethylenetetramine functionalized graphene oxide-alginate beads nanocomposite as efficient sorbent for dye from aqueous solution.					
36563810	5	37	theme	field	903:907	arg1	studies					909:915	The field studies	899:915	The field studies	899:915	The field studies and regeneration of the beads were investigated.					
36563810	2	38	from	reusability	500:510	arg1	removal					535:541	methylene blue (MB) removal	515:541	methylene blue (MB) removal from water	515:552	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	7	39	theme	molecules	1210:1218	arg1	adsorption					1189:1198	the adsorption	1185:1198	the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions	1185:1309	The highest adsorption capacities were observed at pH ~ 5, meanwhile the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions.					
36563810	8	40	theme	dosage	1330:1335	arg1	value					1321:1325	the value	1317:1325	the value of dosage	1317:1335	When the value of dosage is 0.01 g, the equilibrium concentration (mg/g) are maximum at 103, 110, 164, 168 mg/g for AG, AG-GO, AG-HMTA and AG-GO-HMTA, respectively.					
36563810	8	40	theme	dosage	1330:1335	arg1	0.01 g					1340:1345	0.01 g	1340:1345	0.01 g	1340:1345	When the value of dosage is 0.01 g, the equilibrium concentration (mg/g) are maximum at 103, 110, 164, 168 mg/g for AG, AG-GO, AG-HMTA and AG-GO-HMTA, respectively.					
36563810	1	41	theme	calcium	224:230	arg1	AG-GO-HMTA					289:298	AG-GO-HMTA	289:298	AG-GO-HMTA	289:298	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	1	41	theme	calcium	224:230	arg1	composite					278:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite	149:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA)	149:299	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	7	42	dep	faster	1263:1268	arg1	due					1270:1272	due	1270:1272	due	1270:1272	The highest adsorption capacities were observed at pH ~ 5, meanwhile the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions.					
36563810	4	43	theme	equilibrium	740:750	arg1	data					763:766	The equilibrium adsorption data	736:766	The equilibrium adsorption data	736:766	The equilibrium adsorption data was described by the Langmuir and Freundlich isotherms, and the adsorption kinetic and thermodynamic parameters were investigated.					
36563810	6	44	theme	desired	1027:1033	arg1	morphology					1035:1044	this desired morphology	1022:1044	this desired morphology	1022:1044	AG-GO HMTA displays a well-defined porous structure and this desired morphology arising from high quality dispersion of HMTA within the AG-GO matrix.					
36563810	1	45	theme	alginate	232:239	arg1	AG-GO-HMTA					289:298	AG-GO-HMTA	289:298	AG-GO-HMTA	289:298	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	1	45	theme	alginate	232:239	arg1	composite					278:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite	149:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA)	149:299	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	7	46	theme	electrostatic	1284:1296	arg1	interactions					1298:1309	strong electrostatic interactions	1277:1309	strong electrostatic interactions	1277:1309	The highest adsorption capacities were observed at pH ~ 5, meanwhile the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions.					
36563810	3	47	theme	analysis	715:722	arg1	techniques					724:733	structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques	620:733	structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques	620:733	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	7	48	theme	highest	1120:1126	arg1	capacities					1139:1148	The highest adsorption capacities	1116:1148	The highest adsorption capacities	1116:1148	The highest adsorption capacities were observed at pH ~ 5, meanwhile the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions.					
36563810	2	49	theme	initial	449:455	arg1	concentration					461:473	initial dye concentration	449:473	initial dye concentration	449:473	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	3	50	dep	morphological	685:697	arg1	SEM					705:707	SEM	705:707	SEM	705:707	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	3	50	dep	morphological	685:697	arg1	BET					710:712	BET	710:712	BET	710:712	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	0	51	theme	efficient	85:93	arg1	sorbent					95:101	as efficient sorbent	82:101	as efficient sorbent for dye from aqueous solution	82:131	Hexamethylenetetramine functionalized graphene oxide-alginate beads nanocomposite as efficient sorbent for dye from aqueous solution.					
36563810	1	52	theme	hexamethylenetetramine	305:326	arg1	AG-HMTA					371:377	AG-HMTA	371:377	AG-HMTA	371:377	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	1	52	theme	hexamethylenetetramine	305:326	arg1	composite					360:368	hexamethylenetetramine functionalized calcium alginate composite	305:368	hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA)	305:378	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	4	53	theme	kinetic	843:849	arg1	parameters					869:878	the adsorption kinetic and thermodynamic parameters	828:878	the adsorption kinetic and thermodynamic parameters	828:878	The equilibrium adsorption data was described by the Langmuir and Freundlich isotherms, and the adsorption kinetic and thermodynamic parameters were investigated.					
36563810	8	54	from	103	1400:1402	arg1	maximum					1389:1395	maximum	1389:1395	maximum	1389:1395	When the value of dosage is 0.01 g, the equilibrium concentration (mg/g) are maximum at 103, 110, 164, 168 mg/g for AG, AG-GO, AG-HMTA and AG-GO-HMTA, respectively.					
36563810	8	54	from	103	1400:1402	arg1	concentration					1364:1376	the equilibrium concentration	1348:1376	the equilibrium concentration (mg/g)	1348:1383	When the value of dosage is 0.01 g, the equilibrium concentration (mg/g) are maximum at 103, 110, 164, 168 mg/g for AG, AG-GO, AG-HMTA and AG-GO-HMTA, respectively.					
36563810	9	55	theme	first	1512:1516	arg1	order					1518:1522	pseudo first order	1505:1522	pseudo first order	1505:1522	The present work shows that pseudo first order could describe the MB adsorption onto AG while it couldn't describe the MB adsorption onto the functionalized sorbents due to the hybrid materials complicity.					
36563810	6	56	theme	AG-GO	1102:1106	arg1	matrix					1108:1113	the AG-GO matrix	1098:1113	the AG-GO matrix	1098:1113	AG-GO HMTA displays a well-defined porous structure and this desired morphology arising from high quality dispersion of HMTA within the AG-GO matrix.					
36563810	1	57	theme	functionalized	328:341	arg1	AG-HMTA					371:377	AG-HMTA	371:377	AG-HMTA	371:377	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	1	57	theme	functionalized	328:341	arg1	composite					360:368	hexamethylenetetramine functionalized calcium alginate composite	305:368	hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA)	305:378	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	4	58	theme	adsorption	832:841	arg1	parameters					869:878	the adsorption kinetic and thermodynamic parameters	828:878	the adsorption kinetic and thermodynamic parameters	828:878	The equilibrium adsorption data was described by the Langmuir and Freundlich isotherms, and the adsorption kinetic and thermodynamic parameters were investigated.					
36563810	3	59	theme	thermal	656:662	arg1	techniques					724:733	structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques	620:733	structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques	620:733	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	8	60	theme	equilibrium	1352:1362	arg1	mg/g					1379:1382	mg/g	1379:1382	mg/g	1379:1382	When the value of dosage is 0.01 g, the equilibrium concentration (mg/g) are maximum at 103, 110, 164, 168 mg/g for AG, AG-GO, AG-HMTA and AG-GO-HMTA, respectively.					
36563810	8	60	theme	equilibrium	1352:1362	arg1	maximum					1389:1395	maximum	1389:1395	maximum	1389:1395	When the value of dosage is 0.01 g, the equilibrium concentration (mg/g) are maximum at 103, 110, 164, 168 mg/g for AG, AG-GO, AG-HMTA and AG-GO-HMTA, respectively.					
36563810	8	60	theme	equilibrium	1352:1362	arg1	concentration					1364:1376	the equilibrium concentration	1348:1376	the equilibrium concentration (mg/g)	1348:1383	When the value of dosage is 0.01 g, the equilibrium concentration (mg/g) are maximum at 103, 110, 164, 168 mg/g for AG, AG-GO, AG-HMTA and AG-GO-HMTA, respectively.					
36563810	9	61	theme	present	1481:1487	arg1	work					1489:1492	The present work	1477:1492	The present work	1477:1492	The present work shows that pseudo first order could describe the MB adsorption onto AG while it couldn't describe the MB adsorption onto the functionalized sorbents due to the hybrid materials complicity.					
36563810	1	62	theme	calcium	343:349	arg1	AG-HMTA					371:377	AG-HMTA	371:377	AG-HMTA	371:377	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	1	62	theme	calcium	343:349	arg1	composite					360:368	hexamethylenetetramine functionalized calcium alginate composite	305:368	hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA)	305:378	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	4	63	theme	thermodynamic	855:867	arg1	parameters					869:878	the adsorption kinetic and thermodynamic parameters	828:878	the adsorption kinetic and thermodynamic parameters	828:878	The equilibrium adsorption data was described by the Langmuir and Freundlich isotherms, and the adsorption kinetic and thermodynamic parameters were investigated.					
36563810	9	64	theme	MB	1543:1544	arg1	adsorption					1546:1555	the MB adsorption	1539:1555	the MB adsorption onto AG	1539:1563	The present work shows that pseudo first order could describe the MB adsorption onto AG while it couldn't describe the MB adsorption onto the functionalized sorbents due to the hybrid materials complicity.					
36563810	7	65	theme	charged	1232:1238	arg1	molecules					1210:1218	MB dye molecules	1203:1218	MB dye molecules	1203:1218	The highest adsorption capacities were observed at pH ~ 5, meanwhile the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions.					
36563810	1	66	theme	AG	242:243	arg1	AG-GO-HMTA					289:298	AG-GO-HMTA	289:298	AG-GO-HMTA	289:298	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	1	66	theme	AG	242:243	arg1	composite					278:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite	149:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA)	149:299	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	5	67	theme	beads	941:945	arg1	studies					909:915	The field studies	899:915	The field studies	899:915	The field studies and regeneration of the beads were investigated.					
36563810	5	67	theme	beads	941:945	arg1	regeneration					921:932	regeneration	921:932	regeneration of the beads	921:945	The field studies and regeneration of the beads were investigated.					
36563810	0	68	from	solution	124:131	arg1	sorbent					95:101	as efficient sorbent	82:101	as efficient sorbent for dye from aqueous solution	82:131	Hexamethylenetetramine functionalized graphene oxide-alginate beads nanocomposite as efficient sorbent for dye from aqueous solution.					
36563810	6	69	theme	HMTA	1086:1089	arg1	dispersion					1072:1081	high quality dispersion	1059:1081	high quality dispersion of HMTA within the AG-GO matrix	1059:1113	AG-GO HMTA displays a well-defined porous structure and this desired morphology arising from high quality dispersion of HMTA within the AG-GO matrix.					
36563810	2	70	theme	methylene	515:523	arg1	removal					535:541	methylene blue (MB) removal	515:541	methylene blue (MB) removal from water	515:552	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	3	71	dep	XRD	637:639	arg1	e.g.					632:635	e.g.	632:635	e.g.	632:635	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	3	72	theme	structural	620:629	arg1	techniques					724:733	structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques	620:733	structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques	620:733	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	2	73	from	impact	427:432	arg1	removal					535:541	methylene blue (MB) removal	515:541	methylene blue (MB) removal from water	515:552	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	1	74	theme	novel	151:155	arg1	AG-GO-HMTA					289:298	AG-GO-HMTA	289:298	AG-GO-HMTA	289:298	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	1	74	theme	novel	151:155	arg1	composite					278:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite	149:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA)	149:299	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	3	75	dep	SEM	705:707	arg1	e.g.					700:703	e.g.	700:703	e.g.	700:703	The prepared sorbents were characterized using structural (e.g. XRD, FT-IR, EDAX), thermal (e.g. TGA, DTG), and morphological (e.g. SEM, BET) analysis techniques.					
36563810	1	76	theme	immobilized	246:256	arg1	AG-GO-HMTA					289:298	AG-GO-HMTA	289:298	AG-GO-HMTA	289:298	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	1	76	theme	immobilized	246:256	arg1	composite					278:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite	149:286	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA)	149:299	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	7	77	theme	MB	1203:1204	arg1	molecules					1210:1218	MB dye molecules	1203:1218	MB dye molecules	1203:1218	The highest adsorption capacities were observed at pH ~ 5, meanwhile the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions.					
36563810	1	78	theme	alginate	351:358	arg1	AG-HMTA					371:377	AG-HMTA	371:377	AG-HMTA	371:377	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	1	78	theme	alginate	351:358	arg1	composite					360:368	hexamethylenetetramine functionalized calcium alginate composite	305:368	hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA)	305:378	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36563810	2	79	from	water	548:552	arg1	removal					535:541	methylene blue (MB) removal	515:541	methylene blue (MB) removal from water	515:552	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	2	80	from	time	443:446	arg1	removal					535:541	methylene blue (MB) removal	515:541	methylene blue (MB) removal from water	515:552	Adsorption factors including pH impact, contact time, initial dye concentration, dosage, selectivity and reusability on methylene blue (MB) removal from water were investigated.					
36563810	6	81	theme	well-defined	988:999	arg1	structure					1008:1016	a well-defined porous structure	986:1016	a well-defined porous structure	986:1016	AG-GO HMTA displays a well-defined porous structure and this desired morphology arising from high quality dispersion of HMTA within the AG-GO matrix.					
36563810	7	82	theme	dye	1206:1208	arg1	molecules					1210:1218	MB dye molecules	1203:1218	MB dye molecules	1203:1218	The highest adsorption capacities were observed at pH ~ 5, meanwhile the adsorption of MB dye molecules, positively charged, onto the beads became faster due to strong electrostatic interactions.					
36563810	1	83	theme	graphene	258:265	arg1	oxide					267:271	graphene oxide	258:271	a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA)	149:299	In this study, a novel eco-friendly sorbent, hexamethylenetetramine (HMTA) functionalized calcium alginate (AG) immobilized graphene oxide (GO) composite (AG-GO-HMTA) and hexamethylenetetramine functionalized calcium alginate composite (AG-HMTA) were prepared.					
36652966	6	0	theme	cooked	853:858	arg1	waste					880:884	cooked and modified starch waste	853:884	cooked and modified starch waste	853:884	Uncooked starch waste can produce 20 % more acetone than cooked and modified starch waste.					
36652966	7	1	theme	mineral	903:909	arg1	waste					911:915	mineral waste	903:915	mineral waste	903:915	Fatty waste and mineral waste can be digested anaerobically generating biogas.					
36652966	5	2	from	powder	763:768	arg1	concentration					734:746	50 % lower acetone concentration	715:746	50 % lower acetone concentration from milk dust powder than from starchy wastes	715:793	Lactose concentration negatively affected fermentation and led to 50 % lower acetone concentration from milk dust powder than from starchy wastes.					
36652966	2	3	theme	waste	301:305	arg1	samples					314:320	Various industrial food waste stream samples	277:320	Various industrial food waste stream samples	277:320	Various industrial food waste stream samples were characterized with respect to their physico-chemical characteristics and elemental composition.					
36652966	10	4	theme	Low	1267:1269	arg1	10-15 MJ/kg					1290:1300	10-15 MJ/kg	1290:1300	10-15 MJ/kg	1290:1300	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	10	4	theme	Low	1267:1269	arg1	content					1281:1287	Low calorific content	1267:1287	Low calorific content (10-15 MJ/kg) vegetable wastes	1267:1318	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	9	5	theme	soil	1250:1253	arg1	types					1185:1189	these waste types	1173:1189	these waste types	1173:1189	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	9	5	theme	soil	1250:1253	arg1	amendments					1255:1264	soil amendments	1250:1264	soil amendments	1250:1264	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	2	6	theme	industrial	285:294	arg1	samples					314:320	Various industrial food waste stream samples	277:320	Various industrial food waste stream samples	277:320	Various industrial food waste stream samples were characterized with respect to their physico-chemical characteristics and elemental composition.					
36652966	10	7	theme	valuable	1442:1449	arg1	antioxidants					1390:1401	antioxidants	1390:1401	antioxidants	1390:1401	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	10	7	theme	valuable	1442:1449	arg1	flavonoids					1378:1387	flavonoids	1378:1387	flavonoids	1378:1387	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	10	7	theme	valuable	1442:1449	arg1	products					1451:1458	valuable products	1442:1458	valuable products	1442:1458	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	10	7	theme	valuable	1442:1449	arg1	pigments					1407:1414	pigments	1407:1414	pigments	1407:1414	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	5	8	theme	milk	753:756	arg1	powder					763:768	milk dust powder	753:768	milk dust powder	753:768	Lactose concentration negatively affected fermentation and led to 50 % lower acetone concentration from milk dust powder than from starchy wastes.					
36652966	12	9	theme	industry	1662:1669	arg1	knowledge					1671:1679	food industry knowledge	1657:1679	food industry knowledge	1657:1679	These findings will help advance food industry knowledge and improve sustainable food production through valorized processing waste management.					
36652966	9	10	theme	C/N	1060:1062	arg1	ratios					1064:1069	Low C/N ratios	1056:1069	Low C/N ratios of wastewater and solids from food processing waste	1056:1121	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	5	11	theme	lower	720:724	arg1	concentration					734:746	50 % lower acetone concentration	715:746	50 % lower acetone concentration from milk dust powder than from starchy wastes	715:793	Lactose concentration negatively affected fermentation and led to 50 % lower acetone concentration from milk dust powder than from starchy wastes.					
36652966	1	12	theme	waste	141:145	arg1	Valorization					82:93	Valorization	82:93	Valorization	82:93	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	1	12	theme	waste	141:145	arg1	utilization					99:109	utilization	99:109	utilization	99:109	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	11	13	theme	best	1507:1510	arg1	pathway					1525:1531	best valorization pathway	1507:1531	best valorization pathway	1507:1531	A model mapping food waste characteristics to best valorization pathway was developed to guide waste management and future cost and environmental impact analyses.					
36652966	11	14	theme	valorization	1512:1523	arg1	pathway					1525:1531	best valorization pathway	1507:1531	best valorization pathway	1507:1531	A model mapping food waste characteristics to best valorization pathway was developed to guide waste management and future cost and environmental impact analyses.					
36652966	10	15	theme	calorific	1271:1279	arg1	10-15 MJ/kg					1290:1300	10-15 MJ/kg	1290:1300	10-15 MJ/kg	1290:1300	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	10	15	theme	calorific	1271:1279	arg1	content					1281:1287	Low calorific content	1267:1287	Low calorific content (10-15 MJ/kg) vegetable wastes	1267:1318	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	1	16	theme	platform	172:179	arg1	products					162:169	Valorization and utilization of industrial food processing waste as value added products	82:169	Valorization and utilization of industrial food processing waste as value added products	82:169	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	1	16	theme	platform	172:179	arg1	chemicals					181:189	platform chemicals	172:189	platform chemicals	172:189	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	9	17	theme	processing	1106:1115	arg1	waste					1117:1121	food processing waste	1101:1121	food processing waste	1101:1121	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	11	18	theme	food	1477:1480	arg1	characteristics					1488:1502	food waste characteristics	1477:1502	food waste characteristics	1477:1502	A model mapping food waste characteristics to best valorization pathway was developed to guide waste management and future cost and environmental impact analyses.					
36652966	3	19	theme	powder	469:474	arg1	subset					425:430	A subset	423:430	A subset of starchy food wastes and milk dust powder	423:474	A subset of starchy food wastes and milk dust powder were evaluated in batch fermentation to acetone, a useful platform chemical.					
36652966	12	20	theme	processing	1739:1748	arg1	management					1756:1765	valorized processing waste management	1729:1765	valorized processing waste management	1729:1765	These findings will help advance food industry knowledge and improve sustainable food production through valorized processing waste management.					
36652966	3	21	theme	milk	459:462	arg1	powder					469:474	milk dust powder	459:474	milk dust powder	459:474	A subset of starchy food wastes and milk dust powder were evaluated in batch fermentation to acetone, a useful platform chemical.					
36652966	1	22	dep	added	156:160	arg1	Valorization					82:93	Valorization	82:93	Valorization	82:93	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	1	22	dep	added	156:160	arg1	utilization					99:109	utilization	99:109	utilization	99:109	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	9	23	theme	anaerobic	1149:1157	arg1	digestion					1159:1167	anaerobic digestion	1149:1167	anaerobic digestion	1149:1167	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	3	24	theme	batch	494:498	arg1	fermentation					500:511	batch fermentation	494:511	batch fermentation to acetone, a useful platform chemical	494:550	A subset of starchy food wastes and milk dust powder were evaluated in batch fermentation to acetone, a useful platform chemical.					
36652966	0	25	theme	potential	21:29	arg1	valorization					31:42	potential valorization	21:42	potential valorization	21:42	Characterization and potential valorization of industrial food processing wastes.					
36652966	1	26	theme	food	125:128	arg1	waste					141:145	industrial food processing waste	114:145	industrial food processing waste	114:145	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	12	27	theme	food	1705:1708	arg1	production					1710:1719	sustainable food production	1693:1719	sustainable food production	1693:1719	These findings will help advance food industry knowledge and improve sustainable food production through valorized processing waste management.					
36652966	3	28	theme	wastes	448:453	arg1	subset					425:430	A subset	423:430	A subset of starchy food wastes and milk dust powder	423:474	A subset of starchy food wastes and milk dust powder were evaluated in batch fermentation to acetone, a useful platform chemical.					
36652966	10	29	theme	energy	1343:1348	arg1	production					1350:1359	energy production	1343:1359	energy production	1343:1359	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	8	30	theme	Calorific	966:974	arg1	value					976:980	Calorific value	966:980	Calorific value of soybean waste	966:997	Calorific value of soybean waste was 40 MJ/kg sufficiently high for biodiesel production.					
36652966	3	31	theme	starchy	435:441	arg1	wastes					448:453	starchy food wastes	435:453	starchy food wastes	435:453	A subset of starchy food wastes and milk dust powder were evaluated in batch fermentation to acetone, a useful platform chemical.					
36652966	4	32	theme	Production	553:562	arg1	levels					564:569	Production levels	553:569	Production levels	553:569	Production levels were similar to acetone produced from glucose but were achieved more quickly.					
36652966	9	33	theme	waste	1179:1183	arg1	types					1185:1189	these waste types	1173:1189	these waste types	1173:1189	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	9	33	theme	waste	1179:1183	arg1	amendments					1255:1264	soil amendments	1250:1264	soil amendments	1250:1264	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	2	34	theme	physico-chemical	363:378	arg1	characteristics					380:394	their physico-chemical characteristics	357:394	their physico-chemical characteristics	357:394	Various industrial food waste stream samples were characterized with respect to their physico-chemical characteristics and elemental composition.					
36652966	0	35	theme	food	58:61	arg1	processing					63:72	industrial food processing	47:72	industrial food processing	47:72	Characterization and potential valorization of industrial food processing wastes.					
36652966	9	36	theme	wastewater	1074:1083	arg1	ratios					1064:1069	Low C/N ratios	1056:1069	Low C/N ratios of wastewater and solids from food processing waste	1056:1121	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	6	37	theme	modified	864:871	arg1	waste					880:884	cooked and modified starch waste	853:884	cooked and modified starch waste	853:884	Uncooked starch waste can produce 20 % more acetone than cooked and modified starch waste.					
36652966	8	38	theme	waste	993:997	arg1	value					976:980	Calorific value	966:980	Calorific value of soybean waste	966:997	Calorific value of soybean waste was 40 MJ/kg sufficiently high for biodiesel production.					
36652966	9	39	from	waste	1117:1121	arg1	ratios					1064:1069	Low C/N ratios	1056:1069	Low C/N ratios of wastewater and solids from food processing waste	1056:1121	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	9	40	theme	solids	1089:1094	arg1	ratios					1064:1069	Low C/N ratios	1056:1069	Low C/N ratios of wastewater and solids from food processing waste	1056:1121	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	3	41	theme	chemical	543:550	arg1	platform					534:541	a useful platform	525:541	a useful platform chemical	525:550	A subset of starchy food wastes and milk dust powder were evaluated in batch fermentation to acetone, a useful platform chemical.					
36652966	3	41	theme	chemical	543:550	arg1	acetone					516:522	acetone	516:522	acetone	516:522	A subset of starchy food wastes and milk dust powder were evaluated in batch fermentation to acetone, a useful platform chemical.					
36652966	3	42	theme	useful	527:532	arg1	platform					534:541	a useful platform	525:541	a useful platform chemical	525:550	A subset of starchy food wastes and milk dust powder were evaluated in batch fermentation to acetone, a useful platform chemical.					
36652966	3	42	theme	useful	527:532	arg1	acetone					516:522	acetone	516:522	acetone	516:522	A subset of starchy food wastes and milk dust powder were evaluated in batch fermentation to acetone, a useful platform chemical.					
36652966	1	43	theme	management	259:268	arg1	costs					270:274	waste management costs	253:274	waste management costs	253:274	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	2	44	theme	stream	307:312	arg1	samples					314:320	Various industrial food waste stream samples	277:320	Various industrial food waste stream samples	277:320	Various industrial food waste stream samples were characterized with respect to their physico-chemical characteristics and elemental composition.					
36652966	6	45	theme	starch	805:810	arg1	waste					812:816	Uncooked starch waste	796:816	Uncooked starch waste	796:816	Uncooked starch waste can produce 20 % more acetone than cooked and modified starch waste.					
36652966	8	46	theme	biodiesel	1034:1042	arg1	production					1044:1053	biodiesel production	1034:1053	biodiesel production	1034:1053	Calorific value of soybean waste was 40 MJ/kg sufficiently high for biodiesel production.					
36652966	11	47	theme	future	1577:1582	arg1	cost					1584:1587	future cost	1577:1587	future cost	1577:1587	A model mapping food waste characteristics to best valorization pathway was developed to guide waste management and future cost and environmental impact analyses.					
36652966	2	48	theme	food	296:299	arg1	samples					314:320	Various industrial food waste stream samples	277:320	Various industrial food waste stream samples	277:320	Various industrial food waste stream samples were characterized with respect to their physico-chemical characteristics and elemental composition.					
36652966	7	49	theme	Fatty	887:891	arg1	waste					893:897	Fatty waste	887:897	Fatty waste	887:897	Fatty waste and mineral waste can be digested anaerobically generating biogas.					
36652966	12	50	theme	food	1657:1660	arg1	knowledge					1671:1679	food industry knowledge	1657:1679	food industry knowledge	1657:1679	These findings will help advance food industry knowledge and improve sustainable food production through valorized processing waste management.					
36652966	6	51	dep	acetone	840:846	arg1	%					833:833	20 %	830:833	20 %	830:833	Uncooked starch waste can produce 20 % more acetone than cooked and modified starch waste.					
36652966	11	52	theme	environmental	1593:1605	arg1	analyses					1614:1621	environmental impact analyses	1593:1621	environmental impact analyses	1593:1621	A model mapping food waste characteristics to best valorization pathway was developed to guide waste management and future cost and environmental impact analyses.					
36652966	11	53	theme	impact	1607:1612	arg1	analyses					1614:1621	environmental impact analyses	1593:1621	environmental impact analyses	1593:1621	A model mapping food waste characteristics to best valorization pathway was developed to guide waste management and future cost and environmental impact analyses.					
36652966	5	54	theme	dust	758:761	arg1	powder					763:768	milk dust powder	753:768	milk dust powder	753:768	Lactose concentration negatively affected fermentation and led to 50 % lower acetone concentration from milk dust powder than from starchy wastes.					
36652966	5	55	from	wastes	788:793	arg1	concentration					734:746	50 % lower acetone concentration	715:746	50 % lower acetone concentration from milk dust powder than from starchy wastes	715:793	Lactose concentration negatively affected fermentation and led to 50 % lower acetone concentration from milk dust powder than from starchy wastes.					
36652966	9	56	theme	Low	1056:1058	arg1	ratios					1064:1069	Low C/N ratios	1056:1069	Low C/N ratios of wastewater and solids from food processing waste	1056:1121	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	1	57	theme	processing	130:139	arg1	waste					141:145	industrial food processing waste	114:145	industrial food processing waste	114:145	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	5	58	theme	acetone	726:732	arg1	concentration					734:746	50 % lower acetone concentration	715:746	50 % lower acetone concentration from milk dust powder than from starchy wastes	715:793	Lactose concentration negatively affected fermentation and led to 50 % lower acetone concentration from milk dust powder than from starchy wastes.					
36652966	2	59	theme	Various	277:283	arg1	samples					314:320	Various industrial food waste stream samples	277:320	Various industrial food waste stream samples	277:320	Various industrial food waste stream samples were characterized with respect to their physico-chemical characteristics and elemental composition.					
36652966	10	60	theme	content	1281:1287	arg1	wastes					1313:1318	Low calorific content (10-15 MJ/kg) vegetable wastes	1267:1318	Low calorific content (10-15 MJ/kg) vegetable wastes	1267:1318	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	1	61	theme	added	156:160	arg1	biofuels					195:202	biofuels	195:202	biofuels	195:202	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	1	61	theme	added	156:160	arg1	products					162:169	Valorization and utilization of industrial food processing waste as value added products	82:169	Valorization and utilization of industrial food processing waste as value added products	82:169	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	1	61	theme	added	156:160	arg1	chemicals					181:189	platform chemicals	172:189	platform chemicals	172:189	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	11	62	theme	waste	1556:1560	arg1	management					1562:1571	waste management	1556:1571	waste management	1556:1571	A model mapping food waste characteristics to best valorization pathway was developed to guide waste management and future cost and environmental impact analyses.					
36652966	12	63	theme	waste	1750:1754	arg1	management					1756:1765	valorized processing waste management	1729:1765	valorized processing waste management	1729:1765	These findings will help advance food industry knowledge and improve sustainable food production through valorized processing waste management.					
36652966	9	64	theme	food	1101:1104	arg1	waste					1117:1121	food processing waste	1101:1121	food processing waste	1101:1121	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	5	65	theme	starchy	780:786	arg1	wastes					788:793	starchy wastes	780:793	starchy wastes	780:793	Lactose concentration negatively affected fermentation and led to 50 % lower acetone concentration from milk dust powder than from starchy wastes.					
36652966	5	66	theme	Lactose	649:655	arg1	concentration					657:669	Lactose concentration	649:669	Lactose concentration	649:669	Lactose concentration negatively affected fermentation and led to 50 % lower acetone concentration from milk dust powder than from starchy wastes.					
36652966	10	67	from	rich	1370:1373	arg1	antioxidants					1390:1401	antioxidants	1390:1401	antioxidants	1390:1401	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	10	67	from	rich	1370:1373	arg1	flavonoids					1378:1387	flavonoids	1378:1387	flavonoids	1378:1387	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	10	67	from	rich	1370:1373	arg1	products					1451:1458	valuable products	1442:1458	valuable products	1442:1458	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	10	67	from	rich	1370:1373	arg1	pigments					1407:1414	pigments	1407:1414	pigments	1407:1414	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	12	68	theme	valorized	1729:1737	arg1	management					1756:1765	valorized processing waste management	1729:1765	valorized processing waste management	1729:1765	These findings will help advance food industry knowledge and improve sustainable food production through valorized processing waste management.					
36652966	10	69	from	flavonoids	1378:1387	arg1	rich					1370:1373	rich	1370:1373	rich	1370:1373	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	9	70	used	used	1242:1245	arg2	amendments					1255:1264	soil amendments	1250:1264	soil amendments	1250:1264	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	9	70	used	used	1242:1245	arg2	types					1185:1189	these waste types	1173:1189	these waste types	1173:1189	Low C/N ratios of wastewater and solids from food processing waste makes them unsuitable for anaerobic digestion but these waste types can be converted thermochemically to hydrochar and used as soil amendments.					
36652966	3	71	theme	dust	464:467	arg1	powder					469:474	milk dust powder	459:474	milk dust powder	459:474	A subset of starchy food wastes and milk dust powder were evaluated in batch fermentation to acetone, a useful platform chemical.					
36652966	1	72	theme	industrial	114:123	arg1	waste					141:145	industrial food processing waste	114:145	industrial food processing waste	114:145	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36652966	10	73	from	antioxidants	1390:1401	arg1	rich					1370:1373	rich	1370:1373	rich	1370:1373	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	11	74	theme	waste	1482:1486	arg1	characteristics					1488:1502	food waste characteristics	1477:1502	food waste characteristics	1477:1502	A model mapping food waste characteristics to best valorization pathway was developed to guide waste management and future cost and environmental impact analyses.					
36652966	0	75	theme	industrial	47:56	arg1	processing					63:72	industrial food processing	47:72	industrial food processing	47:72	Characterization and potential valorization of industrial food processing wastes.					
36652966	12	76	theme	sustainable	1693:1703	arg1	production					1710:1719	sustainable food production	1693:1719	sustainable food production	1693:1719	These findings will help advance food industry knowledge and improve sustainable food production through valorized processing waste management.					
36652966	10	77	from	pigments	1407:1414	arg1	rich					1370:1373	rich	1370:1373	rich	1370:1373	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	0	78	theme	processing	63:72	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and potential valorization of industrial food processing wastes.					
36652966	0	78	theme	processing	63:72	arg1	valorization					31:42	potential valorization	21:42	potential valorization	21:42	Characterization and potential valorization of industrial food processing wastes.					
36652966	3	79	theme	food	443:446	arg1	wastes					448:453	starchy food wastes	435:453	starchy food wastes	435:453	A subset of starchy food wastes and milk dust powder were evaluated in batch fermentation to acetone, a useful platform chemical.					
36652966	6	80	theme	Uncooked	796:803	arg1	waste					812:816	Uncooked starch waste	796:816	Uncooked starch waste	796:816	Uncooked starch waste can produce 20 % more acetone than cooked and modified starch waste.					
36652966	6	81	theme	starch	873:878	arg1	waste					880:884	cooked and modified starch waste	853:884	cooked and modified starch waste	853:884	Uncooked starch waste can produce 20 % more acetone than cooked and modified starch waste.					
36652966	10	82	theme	vegetable	1303:1311	arg1	wastes					1313:1318	Low calorific content (10-15 MJ/kg) vegetable wastes	1267:1318	Low calorific content (10-15 MJ/kg) vegetable wastes	1267:1318	Low calorific content (10-15 MJ/kg) vegetable wastes also are not ideal for energy production, but are rich in flavonoids, antioxidants and pigments which can be extracted as valuable products.					
36652966	8	83	theme	soybean	985:991	arg1	waste					993:997	soybean waste	985:997	soybean waste	985:997	Calorific value of soybean waste was 40 MJ/kg sufficiently high for biodiesel production.					
36652966	2	84	theme	elemental	400:408	arg1	composition					410:420	elemental composition	400:420	elemental composition	400:420	Various industrial food waste stream samples were characterized with respect to their physico-chemical characteristics and elemental composition.					
36652966	1	85	theme	waste	253:257	arg1	costs					270:274	waste management costs	253:274	waste management costs	253:274	Valorization and utilization of industrial food processing waste as value added products, platform chemicals and biofuels, are needed to improve sustainability and reduce waste management costs.					
36210454	0	0	theme	experimental	77:88	arg1	warming					90:96	experimental warming	77:96	experimental warming	77:96	Carbohydrates and secondary compounds of alpine tundra shrubs in relation to experimental warming.					
36210454	3	1	theme	dwarf	702:706	arg1	shrubs					708:713	three dominant dwarf shrubs	687:713	three dominant dwarf shrubs	687:713	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	16	2	theme	secondary	1749:1757	arg1	compounds					1759:1767	secondary compounds	1749:1767	secondary compounds	1749:1767	asiatica was companied by increased trend in carbohydrate concentrations and decreased ratio of secondary compounds to total carbon in the warming OTCs.					
36210454	14	3	contain	had	1465:1467	arg2	carbon					1521:1526	invested more carbon	1507:1526	invested more carbon	1507:1526	confertissimum had significantly lower carbohydrates and invested more carbon on secondary compounds than the other two species.					
36210454	14	3	contain	had	1465:1467	arg1	confertissimum					1450:1463	confertissimum	1450:1463	confertissimum	1450:1463	confertissimum had significantly lower carbohydrates and invested more carbon on secondary compounds than the other two species.					
36210454	14	3	contain	had	1465:1467	arg2	carbohydrates					1489:1501	significantly lower carbohydrates	1469:1501	significantly lower carbohydrates	1469:1501	confertissimum had significantly lower carbohydrates and invested more carbon on secondary compounds than the other two species.					
36210454	16	4	theme	increased	1679:1687	arg1	trend					1689:1693	increased trend	1679:1693	increased trend in carbohydrate concentrations and decreased ratio of secondary compounds to total carbon in the warming OTCs	1679:1803	asiatica was companied by increased trend in carbohydrate concentrations and decreased ratio of secondary compounds to total carbon in the warming OTCs.					
36210454	16	5	theme	carbohydrate	1698:1709	arg1	concentrations					1711:1724	carbohydrate concentrations	1698:1724	carbohydrate concentrations	1698:1724	asiatica was companied by increased trend in carbohydrate concentrations and decreased ratio of secondary compounds to total carbon in the warming OTCs.					
36210454	7	6	theme	negative	1107:1114	arg1	relationship					1116:1127	negative relationship	1107:1127	negative relationship	1107:1127	No significant trade-off or negative relationship between carbohydrates and secondary compounds was observed.					
36210454	1	7	theme	response	156:163	arg1	direction					165:173	response direction	156:173	response direction	156:173	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	3	8	theme	total	621:625	arg1	phenols					627:633	total phenols	621:633	total phenols	621:633	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	9	from	compounds	610:618	arg1	shrubs					708:713	three dominant dwarf shrubs	687:713	three dominant dwarf shrubs	687:713	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	0	10	from	Carbohydrates	0:12	arg1	relation					65:72	relation	65:72	relation to experimental warming	65:96	Carbohydrates and secondary compounds of alpine tundra shrubs in relation to experimental warming.					
36210454	18	11	theme	ecosystem	2079:2087	arg1	structure					2046:2054	community structure	2036:2054	community structure	2036:2054	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	18	11	theme	ecosystem	2079:2087	arg1	species					2012:2018	species composition and community structure	2012:2054	species composition and community structure of the Changbai tundra ecosystem under future climate warming	2012:2116	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	18	11	theme	ecosystem	2079:2087	arg1	composition					2020:2030	composition	2020:2030	composition	2020:2030	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	4	12	dep	Vaccinium	780:788	arg1	uliginosum					790:799	Vaccinium uliginosum	780:799	Vaccinium uliginosum	780:799	asiatica, Rhododendron confertissimum and Vaccinium uliginosum, were investigated during the growing season.					
36210454	8	13	theme	octopetala	1205:1214	arg1	var					1216:1218	Dr. octopetala var	1201:1218	Dr. octopetala var	1201:1218	Compared to Dr. octopetala var.					
36210454	3	14	theme	nonstructural	540:552	arg1	coverage					530:537	the vegetation coverage	515:537	the vegetation coverage	515:537	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	14	theme	nonstructural	540:552	arg1	carbohydrates					554:566	nonstructural carbohydrates	540:566	nonstructural carbohydrates (soluble sugars and starch)	540:594	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	15	15	theme	var	1648:1650	arg1	competitiveness					1614:1628	competitiveness	1614:1628	competitiveness	1614:1628	CONCLUSIONS Enhanced dominance and competitiveness of Dr. octopetala var.					
36210454	15	15	theme	var	1648:1650	arg1	dominance					1600:1608	dominance	1600:1608	dominance	1600:1608	CONCLUSIONS Enhanced dominance and competitiveness of Dr. octopetala var.					
36210454	7	16	theme	secondary	1155:1163	arg1	compounds					1165:1173	secondary compounds	1155:1173	secondary compounds	1155:1173	No significant trade-off or negative relationship between carbohydrates and secondary compounds was observed.					
36210454	3	17	theme	vegetation	519:528	arg1	var					733:735	var	733:735	var	733:735	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	17	theme	vegetation	519:528	arg1	compounds					610:618	secondary compounds	600:618	secondary compounds (total phenols, flavonoids and triterpenes)	600:662	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	17	theme	vegetation	519:528	arg1	coverage					530:537	the vegetation coverage	515:537	the vegetation coverage	515:537	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	17	theme	vegetation	519:528	arg1	carbohydrates					554:566	nonstructural carbohydrates	540:566	nonstructural carbohydrates (soluble sugars and starch)	540:594	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	10	18	theme	var	1354:1356	arg1	coverage					1327:1334	the coverage	1323:1334	the coverage of Dr. octopetala var	1323:1356	Warming significantly increased the coverage of Dr. octopetala var.					
36210454	16	19	from	carbon	1778:1783	arg1	OTCs					1800:1803	the warming OTCs	1788:1803	the warming OTCs	1788:1803	asiatica was companied by increased trend in carbohydrate concentrations and decreased ratio of secondary compounds to total carbon in the warming OTCs.					
36210454	3	20	theme	dominant	693:700	arg1	shrubs					708:713	three dominant dwarf shrubs	687:713	three dominant dwarf shrubs	687:713	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	14	21	theme	secondary	1531:1539	arg1	compounds					1541:1549	secondary compounds	1531:1549	secondary compounds	1531:1549	confertissimum had significantly lower carbohydrates and invested more carbon on secondary compounds than the other two species.					
36210454	14	22	theme	invested	1507:1514	arg1	carbon					1521:1526	invested more carbon	1507:1526	invested more carbon	1507:1526	confertissimum had significantly lower carbohydrates and invested more carbon on secondary compounds than the other two species.					
36210454	3	23	theme	roots	678:682	arg1	compounds					610:618	secondary compounds	600:618	secondary compounds (total phenols, flavonoids and triterpenes)	600:662	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	23	theme	roots	678:682	arg1	coverage					530:537	the vegetation coverage	515:537	the vegetation coverage	515:537	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	23	theme	roots	678:682	arg1	carbohydrates					554:566	nonstructural carbohydrates	540:566	nonstructural carbohydrates (soluble sugars and starch)	540:594	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	16	24	theme	warming	1792:1798	arg1	OTCs					1800:1803	the warming OTCs	1788:1803	the warming OTCs	1788:1803	asiatica was companied by increased trend in carbohydrate concentrations and decreased ratio of secondary compounds to total carbon in the warming OTCs.					
36210454	14	25	from	carbohydrates	1489:1501	arg1	compounds					1541:1549	secondary compounds	1531:1549	secondary compounds	1531:1549	confertissimum had significantly lower carbohydrates and invested more carbon on secondary compounds than the other two species.					
36210454	2	26	theme	alpine	450:455	arg1	tundra					457:462	Changbai Mountain alpine tundra	432:462	Changbai Mountain alpine tundra	432:462	RESULTS Open-top chambers (OTCs) were used to passively increase air and soil temperatures on Changbai Mountain alpine tundra.					
36210454	16	27	theme	decreased	1730:1738	arg1	ratio					1740:1744	decreased ratio	1730:1744	decreased ratio of secondary compounds to total carbon in the warming OTCs	1730:1803	asiatica was companied by increased trend in carbohydrate concentrations and decreased ratio of secondary compounds to total carbon in the warming OTCs.					
36210454	4	28	theme	growing	831:837	arg1	season					839:844	the growing season	827:844	the growing season	827:844	asiatica, Rhododendron confertissimum and Vaccinium uliginosum, were investigated during the growing season.					
36210454	18	29	theme	dominant	1889:1896	arg1	status					1898:1903	dominant status	1889:1903	dominant status	1889:1903	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	2	30	dep	RESULTS	338:344	arg1	OTCs					365:368	OTCs	365:368	OTCs	365:368	RESULTS Open-top chambers (OTCs) were used to passively increase air and soil temperatures on Changbai Mountain alpine tundra.					
36210454	2	30	dep	RESULTS	338:344	arg1	chambers					355:362	Open-top chambers	346:362	RESULTS Open-top chambers (OTCs)	338:369	RESULTS Open-top chambers (OTCs) were used to passively increase air and soil temperatures on Changbai Mountain alpine tundra.					
36210454	4	31	dep	Rhododendron	748:759	arg1	confertissimum					761:774	Rhododendron confertissimum	748:774	Rhododendron confertissimum	748:774	asiatica, Rhododendron confertissimum and Vaccinium uliginosum, were investigated during the growing season.					
36210454	2	32	theme	Mountain	441:448	arg1	tundra					457:462	Changbai Mountain alpine tundra	432:462	Changbai Mountain alpine tundra	432:462	RESULTS Open-top chambers (OTCs) were used to passively increase air and soil temperatures on Changbai Mountain alpine tundra.					
36210454	7	33	theme	significant	1082:1092	arg1	trade-off					1094:1102	significant trade-off	1082:1102	significant trade-off	1082:1102	No significant trade-off or negative relationship between carbohydrates and secondary compounds was observed.					
36210454	5	34	theme	total	934:938	arg1	phenols					940:946	total phenols	934:946	total phenols for the three species	934:968	Warming did not significantly affect the concentrations of carbohydrates but decreased total phenols for the three species.					
36210454	14	35	theme	lower	1483:1487	arg1	carbohydrates					1489:1501	significantly lower carbohydrates	1469:1501	significantly lower carbohydrates	1469:1501	confertissimum had significantly lower carbohydrates and invested more carbon on secondary compounds than the other two species.					
36210454	2	36	theme	Changbai	432:439	arg1	tundra					457:462	Changbai Mountain alpine tundra	432:462	Changbai Mountain alpine tundra	432:462	RESULTS Open-top chambers (OTCs) were used to passively increase air and soil temperatures on Changbai Mountain alpine tundra.					
36210454	18	37	theme	competition	1914:1924	arg1	ability					1926:1932	the competition ability	1910:1932	the competition ability of V. uliginosum	1910:1949	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	1	38	theme	carbohydrates	192:204	arg1	magnitude					179:187	magnitude	179:187	magnitude	179:187	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	1	38	theme	carbohydrates	192:204	arg1	sensitivity					143:153	sensitivity	143:153	sensitivity	143:153	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	1	38	theme	carbohydrates	192:204	arg1	direction					165:173	response direction	156:173	response direction	156:173	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	0	39	theme	secondary	18:26	arg1	compounds					28:36	secondary compounds	18:36	secondary compounds	18:36	Carbohydrates and secondary compounds of alpine tundra shrubs in relation to experimental warming.					
36210454	1	40	theme	tundra	290:295	arg1	ecosystem					297:305	the tundra ecosystem	286:305	the tundra ecosystem	286:305	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	18	41	theme	Changbai	2063:2070	arg1	ecosystem					2079:2087	the Changbai tundra ecosystem	2059:2087	the Changbai tundra ecosystem	2059:2087	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	1	42	theme	compounds	220:228	arg1	magnitude					179:187	magnitude	179:187	magnitude	179:187	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	1	42	theme	compounds	220:228	arg1	sensitivity					143:153	sensitivity	143:153	sensitivity	143:153	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	1	42	theme	compounds	220:228	arg1	direction					165:173	response direction	156:173	response direction	156:173	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	1	43	dep	sensitivity	143:153	arg1	the					139:141	the	139:141	the	139:141	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	3	44	dep	carbohydrates	554:566	arg1	sugars					577:582	soluble sugars	569:582	soluble sugars	569:582	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	44	dep	carbohydrates	554:566	arg1	starch					588:593	starch	588:593	starch	588:593	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	2	45	theme	soil	411:414	arg1	temperatures					416:427	air and soil temperatures	403:427	air and soil temperatures on Changbai Mountain alpine tundra	403:462	RESULTS Open-top chambers (OTCs) were used to passively increase air and soil temperatures on Changbai Mountain alpine tundra.					
36210454	0	46	theme	alpine	41:46	arg1	shrubs					55:60	alpine tundra shrubs	41:60	alpine tundra shrubs	41:60	Carbohydrates and secondary compounds of alpine tundra shrubs in relation to experimental warming.					
36210454	1	47	theme	ecosystem	297:305	arg1	structure					260:268	structure	260:268	structure	260:268	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	1	47	theme	ecosystem	297:305	arg1	function					274:281	function	274:281	function	274:281	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	6	48	theme	species-specific	1051:1066	arg1	variation					1068:1076	species-specific variation	1051:1076	species-specific variation	1051:1076	Carbohydrates and secondary compounds showed significantly seasonal pattern and species-specific variation.					
36210454	18	49	dep	species	2012:2018	arg1	structure					2046:2054	community structure	2036:2054	community structure	2036:2054	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	18	49	dep	species	2012:2018	arg1	species					2012:2018	species composition and community structure	2012:2054	species composition and community structure of the Changbai tundra ecosystem under future climate warming	2012:2116	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	18	49	dep	species	2012:2018	arg1	composition					2020:2030	composition	2020:2030	composition	2020:2030	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	1	50	theme	secondary	210:218	arg1	compounds					220:228	secondary compounds	210:228	secondary compounds	210:228	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	18	51	theme	climate	2102:2108	arg1	warming					2110:2116	future climate warming	2095:2116	future climate warming	2095:2116	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	18	52	theme	tundra	2072:2077	arg1	ecosystem					2079:2087	the Changbai tundra ecosystem	2059:2087	the Changbai tundra ecosystem	2059:2087	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	6	53	theme	secondary	989:997	arg1	compounds					999:1007	secondary compounds	989:1007	secondary compounds	989:1007	Carbohydrates and secondary compounds showed significantly seasonal pattern and species-specific variation.					
36210454	16	54	from	trend	1689:1693	arg1	concentrations					1711:1724	carbohydrate concentrations	1698:1724	carbohydrate concentrations	1698:1724	asiatica was companied by increased trend in carbohydrate concentrations and decreased ratio of secondary compounds to total carbon in the warming OTCs.					
36210454	16	54	from	trend	1689:1693	arg1	ratio					1740:1744	decreased ratio	1730:1744	decreased ratio of secondary compounds to total carbon in the warming OTCs	1730:1803	asiatica was companied by increased trend in carbohydrate concentrations and decreased ratio of secondary compounds to total carbon in the warming OTCs.					
36210454	2	55	theme	air	403:405	arg1	temperatures					416:427	air and soil temperatures	403:427	air and soil temperatures on Changbai Mountain alpine tundra	403:462	RESULTS Open-top chambers (OTCs) were used to passively increase air and soil temperatures on Changbai Mountain alpine tundra.					
36210454	0	56	theme	shrubs	55:60	arg1	Carbohydrates					0:12	Carbohydrates	0:12	Carbohydrates	0:12	Carbohydrates and secondary compounds of alpine tundra shrubs in relation to experimental warming.					
36210454	0	56	theme	shrubs	55:60	arg1	compounds					28:36	secondary compounds	18:36	secondary compounds	18:36	Carbohydrates and secondary compounds of alpine tundra shrubs in relation to experimental warming.					
36210454	1	57	theme	future	315:320	arg1	change					330:335	future climate change	315:335	future climate change	315:335	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	16	58	theme	compounds	1759:1767	arg1	concentrations					1711:1724	carbohydrate concentrations	1698:1724	carbohydrate concentrations	1698:1724	asiatica was companied by increased trend in carbohydrate concentrations and decreased ratio of secondary compounds to total carbon in the warming OTCs.					
36210454	16	58	theme	compounds	1759:1767	arg1	ratio					1740:1744	decreased ratio	1730:1744	decreased ratio of secondary compounds to total carbon in the warming OTCs	1730:1803	asiatica was companied by increased trend in carbohydrate concentrations and decreased ratio of secondary compounds to total carbon in the warming OTCs.					
36210454	3	59	theme	continuous	484:493	arg1	warming					495:501	seven years' continuous warming	471:501	seven years' continuous warming (+ 1.5 °C)	471:512	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	59	theme	continuous	484:493	arg1	°C					510:511	1.5 °C	506:511	1.5 °C	506:511	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	0	60	theme	tundra	48:53	arg1	shrubs					55:60	alpine tundra shrubs	41:60	alpine tundra shrubs	41:60	Carbohydrates and secondary compounds of alpine tundra shrubs in relation to experimental warming.					
36210454	1	61	theme	climate	322:328	arg1	change					330:335	future climate change	315:335	future climate change	315:335	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	3	62	theme	leaves	667:672	arg1	compounds					610:618	secondary compounds	600:618	secondary compounds (total phenols, flavonoids and triterpenes)	600:662	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	62	theme	leaves	667:672	arg1	coverage					530:537	the vegetation coverage	515:537	the vegetation coverage	515:537	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	62	theme	leaves	667:672	arg1	carbohydrates					554:566	nonstructural carbohydrates	540:566	nonstructural carbohydrates (soluble sugars and starch)	540:594	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	63	theme	soluble	569:575	arg1	sugars					577:582	soluble sugars	569:582	soluble sugars	569:582	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	18	64	theme	community	2036:2044	arg1	structure					2046:2054	community structure	2036:2054	community structure	2036:2054	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	18	64	theme	community	2036:2044	arg1	species					2012:2018	species composition and community structure	2012:2054	species composition and community structure of the Changbai tundra ecosystem under future climate warming	2012:2116	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	2	65	theme	Open-top	346:353	arg1	OTCs					365:368	OTCs	365:368	OTCs	365:368	RESULTS Open-top chambers (OTCs) were used to passively increase air and soil temperatures on Changbai Mountain alpine tundra.					
36210454	2	65	theme	Open-top	346:353	arg1	chambers					355:362	Open-top chambers	346:362	RESULTS Open-top chambers (OTCs)	338:369	RESULTS Open-top chambers (OTCs) were used to passively increase air and soil temperatures on Changbai Mountain alpine tundra.					
36210454	18	66	theme	uliginosum	1940:1949	arg1	ability					1926:1932	the competition ability	1910:1932	the competition ability of V. uliginosum	1910:1949	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	16	67	theme	total	1772:1776	arg1	carbon					1778:1783	total carbon	1772:1783	total carbon in the warming OTCs	1772:1803	asiatica was companied by increased trend in carbohydrate concentrations and decreased ratio of secondary compounds to total carbon in the warming OTCs.					
36210454	5	68	theme	carbohydrates	906:918	arg1	concentrations					888:901	the concentrations	884:901	the concentrations of carbohydrates	884:918	Warming did not significantly affect the concentrations of carbohydrates but decreased total phenols for the three species.					
36210454	14	69	theme	other	1560:1564	arg1	species					1570:1576	the other two species	1556:1576	the other two species	1556:1576	confertissimum had significantly lower carbohydrates and invested more carbon on secondary compounds than the other two species.					
36210454	3	70	dep	compounds	610:618	arg1	flavonoids					636:645	flavonoids	636:645	flavonoids	636:645	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	70	dep	compounds	610:618	arg1	phenols					627:633	total phenols	621:633	total phenols	621:633	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	70	dep	compounds	610:618	arg1	triterpenes					651:661	triterpenes	651:661	triterpenes	651:661	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	71	theme	secondary	600:608	arg1	compounds					610:618	secondary compounds	600:618	secondary compounds (total phenols, flavonoids and triterpenes)	600:662	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	71	theme	secondary	600:608	arg1	coverage					530:537	the vegetation coverage	515:537	the vegetation coverage	515:537	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	2	72	used	used	376:379	arg2	RESULTS					338:344	RESULTS	338:344	RESULTS Open-top chambers (OTCs)	338:369	RESULTS Open-top chambers (OTCs) were used to passively increase air and soil temperatures on Changbai Mountain alpine tundra.					
36210454	18	73	from	changes	2001:2007	arg1	structure					2046:2054	community structure	2036:2054	community structure	2036:2054	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	18	73	from	changes	2001:2007	arg1	species					2012:2018	species composition and community structure	2012:2054	species composition and community structure of the Changbai tundra ecosystem under future climate warming	2012:2116	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	18	73	from	changes	2001:2007	arg1	composition					2020:2030	composition	2020:2030	composition	2020:2030	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	6	74	theme	seasonal	1030:1037	arg1	pattern					1039:1045	seasonal pattern	1030:1045	seasonal pattern	1030:1045	Carbohydrates and secondary compounds showed significantly seasonal pattern and species-specific variation.					
36210454	1	75	dep	structure	260:268	arg1	the					256:258	the	256:258	the	256:258	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	9	76	theme	secondary	1270:1278	arg1	compounds					1280:1288	secondary compounds	1270:1288	secondary compounds	1270:1288	asiatica, V. uliginosum allocated more carbon on secondary compounds.					
36210454	3	77	dep	coverage	530:537	arg1	warming					495:501	seven years' continuous warming	471:501	seven years' continuous warming (+ 1.5 °C)	471:512	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	3	77	dep	coverage	530:537	arg1	°C					510:511	1.5 °C	506:511	1.5 °C	506:511	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	1	78	dep	BACKGROUND	99:108	arg1	critical					116:123	critical	116:123	critical	116:123	BACKGROUND It is critical to understand the sensitivity, response direction and magnitude of carbohydrates and secondary compounds to warming for predicting the structure and function of the tundra ecosystem towards future climate change.					
36210454	3	79	from	carbohydrates	554:566	arg1	shrubs					708:713	three dominant dwarf shrubs	687:713	three dominant dwarf shrubs	687:713	After seven years' continuous warming (+ 1.5 °C), the vegetation coverage, nonstructural carbohydrates (soluble sugars and starch) and secondary compounds (total phenols, flavonoids and triterpenes) of leaves and roots in three dominant dwarf shrubs, Dryas octopetala var.					
36210454	18	80	theme	future	2095:2100	arg1	warming					2110:2116	future climate warming	2095:2116	future climate warming	2095:2116	asiatica will continue to maintain dominant status, but the competition ability of V. uliginosum could gradually decrease with warming, leading to changes in species composition and community structure of the Changbai tundra ecosystem under future climate warming.					
36210454	0	81	from	compounds	28:36	arg1	relation					65:72	relation	65:72	relation to experimental warming	65:96	Carbohydrates and secondary compounds of alpine tundra shrubs in relation to experimental warming.					
36210454	2	82	from	temperatures	416:427	arg1	tundra					457:462	Changbai Mountain alpine tundra	432:462	Changbai Mountain alpine tundra	432:462	RESULTS Open-top chambers (OTCs) were used to passively increase air and soil temperatures on Changbai Mountain alpine tundra.					
36210454	14	83	from	carbon	1521:1526	arg1	compounds					1541:1549	secondary compounds	1531:1549	secondary compounds	1531:1549	confertissimum had significantly lower carbohydrates and invested more carbon on secondary compounds than the other two species.					
36210454	8	84	theme	Dr.	1201:1203	arg1	var					1216:1218	Dr. octopetala var	1201:1218	Dr. octopetala var	1201:1218	Compared to Dr. octopetala var.					
36907306	6	0	theme	sensitizing	1382:1392	arg1	role					1394:1397	a photothermal sensitizing role	1367:1397	a photothermal sensitizing role	1367:1397	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	1	theme	@	1258:1258	arg1	nanoplatform					1280:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	1	2	from	pH	220:221	arg1	cells					202:206	tumor cells	196:206	tumor cells	196:206	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	4	3	theme	electron	733:740	arg1	transfer					742:749	electron transfer	733:749	electron transfer	733:749	The addition of Au determines the formation of oxygen vacancies, accelerates electron transfer, and enhances redox activity, thus significantly enhancing the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme.					
36907306	6	4	theme	cobalt	1474:1479	arg1	therapy					1507:1513	cobalt ion-mediated chemodynamic therapy	1474:1513	cobalt ion-mediated chemodynamic therapy (CDT)	1474:1519	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	4	theme	cobalt	1474:1479	arg1	activity					1464:1471	the enzyme catalytic activity	1443:1471	the enzyme catalytic activity	1443:1471	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	4	theme	cobalt	1474:1479	arg1	CDT					1516:1518	CDT	1516:1518	CDT	1516:1518	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	0	5	theme	reactive	101:108	arg1	oxygen					110:115	reactive oxygen	101:115	reactive oxygen	101:115	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen species-augmented antitumor therapy.					
36907306	6	6	theme	Co3O4	1259:1263	arg1	nanoplatform					1280:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	0	7	theme	antitumor	135:143	arg1	therapy					145:151	antitumor therapy	135:151	antitumor therapy	135:151	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen species-augmented antitumor therapy.					
36907306	4	8	theme	nanozyme	898:905	arg1	activities					880:889	the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities	810:889	the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme	810:905	The addition of Au determines the formation of oxygen vacancies, accelerates electron transfer, and enhances redox activity, thus significantly enhancing the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme.					
36907306	6	9	theme	near-infrared	1213:1225	arg1	NIR					1228:1230	NIR	1228:1230	NIR	1228:1230	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	9	theme	near-infrared	1213:1225	arg1	irradiation					1239:1249	near-infrared (NIR) light irradiation	1213:1249	near-infrared (NIR) light irradiation	1213:1249	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	5	10	theme	targeting	1116:1124	arg1	ability					1126:1132	the tumor targeting ability	1106:1132	the tumor targeting ability of the nanoplatform	1106:1152	Subsequently, we camouflaged the nanozyme using a biomineralized CaCO3 shell to avoid damage to normal tissues by the nanozyme while effectively encapsulating the photosensitizer IR820, and finally the tumor targeting ability of the nanoplatform was enhanced by the modification of hyaluronic acid.					
36907306	6	11	theme	@	1276:1276	arg1	nanoplatform					1280:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	12	theme	generation	1643:1652	arg1	enhancement					1598:1608	the synergistic enhancement	1582:1608	the synergistic enhancement of reactive oxygen species (ROS) generation	1582:1652	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	13	with	treatment	1317:1325	arg1	imaging					1343:1349	multimodal imaging	1332:1349	multimodal imaging	1332:1349	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	1	14	theme	chemodynamic	309:320	arg1	therapy					322:328	chemodynamic therapy	309:328	chemodynamic therapy	309:328	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	6	15	theme	catalytic	1454:1462	arg1	therapy					1507:1513	cobalt ion-mediated chemodynamic therapy	1474:1513	cobalt ion-mediated chemodynamic therapy (CDT)	1474:1519	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	15	theme	catalytic	1454:1462	arg1	activity					1464:1471	the enzyme catalytic activity	1443:1471	the enzyme catalytic activity	1443:1471	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	15	theme	catalytic	1454:1462	arg1	therapy					1553:1559	IR820-mediated photodynamic therapy	1525:1559	IR820-mediated photodynamic therapy (PDT)	1525:1565	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	16	theme	CaCO3/IR820	1265:1275	arg1	nanoplatform					1280:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	4	17	theme	superoxide	814:823	arg1	SOD					836:838	SOD	836:838	SOD	836:838	The addition of Au determines the formation of oxygen vacancies, accelerates electron transfer, and enhances redox activity, thus significantly enhancing the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme.					
36907306	4	17	theme	superoxide	814:823	arg1	dismutase					825:833	the superoxide dismutase	810:833	the superoxide dismutase (SOD)	810:839	The addition of Au determines the formation of oxygen vacancies, accelerates electron transfer, and enhances redox activity, thus significantly enhancing the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme.					
36907306	1	18	theme	unsuitable	209:218	arg1	pH					220:221	unsuitable pH	209:221	unsuitable pH	209:221	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	6	19	theme	various	1407:1413	arg1	strategies					1415:1424	various strategies	1407:1424	various strategies	1407:1424	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	20	theme	@	1264:1264	arg1	nanoplatform					1280:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	21	theme	enzyme	1447:1452	arg1	therapy					1507:1513	cobalt ion-mediated chemodynamic therapy	1474:1513	cobalt ion-mediated chemodynamic therapy (CDT)	1474:1519	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	21	theme	enzyme	1447:1452	arg1	activity					1464:1471	the enzyme catalytic activity	1443:1471	the enzyme catalytic activity	1443:1471	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	21	theme	enzyme	1447:1452	arg1	therapy					1553:1559	IR820-mediated photodynamic therapy	1525:1559	IR820-mediated photodynamic therapy (PDT)	1525:1565	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	2	22	theme	composite	431:439	arg1	nanoplatform					441:452	a composite nanoplatform	429:452	a composite nanoplatform capable of targeting tumors and selectively degrading in the tumor microenvironment (TME)	429:542	For this purpose, we designed a composite nanoplatform capable of targeting tumors and selectively degrading in the tumor microenvironment (TME) to address these issues.					
36907306	0	23	theme	vacancy-enhanced	7:22	arg1	activity					34:41	Oxygen vacancy-enhanced catalytic activity	0:41	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen	0:115	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen species-augmented antitumor therapy.					
36907306	6	24	theme	photothermal	1369:1380	arg1	role					1394:1397	a photothermal sensitizing role	1367:1397	a photothermal sensitizing role	1367:1397	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	4	25	theme	catalytic	870:878	arg1	activities					880:889	the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities	810:889	the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme	810:905	The addition of Au determines the formation of oxygen vacancies, accelerates electron transfer, and enhances redox activity, thus significantly enhancing the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme.					
36907306	1	26	from	content	185:191	arg1	cells					202:206	tumor cells	196:206	tumor cells	196:206	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	3	27	theme	@	600:600	arg1	nanozyme					607:614	Au@Co3O4 nanozyme	598:614	Au@Co3O4 nanozyme inspired by crystal defect engineering	598:653	In this work, we synthesized Au@Co3O4 nanozyme inspired by crystal defect engineering.					
36907306	5	28	theme	hyaluronic	1190:1199	arg1	acid					1201:1204	hyaluronic acid	1190:1204	hyaluronic acid	1190:1204	Subsequently, we camouflaged the nanozyme using a biomineralized CaCO3 shell to avoid damage to normal tissues by the nanozyme while effectively encapsulating the photosensitizer IR820, and finally the tumor targeting ability of the nanoplatform was enhanced by the modification of hyaluronic acid.					
36907306	4	29	theme	oxygen	703:708	arg1	vacancies					710:718	oxygen vacancies	703:718	oxygen vacancies	703:718	The addition of Au determines the formation of oxygen vacancies, accelerates electron transfer, and enhances redox activity, thus significantly enhancing the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme.					
36907306	0	30	theme	Oxygen	0:5	arg1	activity					34:41	Oxygen vacancy-enhanced catalytic activity	0:41	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen	0:115	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen species-augmented antitumor therapy.					
36907306	1	31	theme	therapy	322:328	arg1	efficiency					295:304	the efficiency	291:304	the efficiency of chemodynamic therapy	291:328	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	4	32	theme	-like	864:868	arg1	activities					880:889	the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities	810:889	the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme	810:905	The addition of Au determines the formation of oxygen vacancies, accelerates electron transfer, and enhances redox activity, thus significantly enhancing the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme.					
36907306	6	33	theme	species	1629:1635	arg1	generation					1643:1652	reactive oxygen species (ROS) generation	1613:1652	reactive oxygen species (ROS) generation	1613:1652	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	3	34	theme	Co3O4	601:605	arg1	nanozyme					607:614	Au@Co3O4 nanozyme	598:614	Au@Co3O4 nanozyme inspired by crystal defect engineering	598:653	In this work, we synthesized Au@Co3O4 nanozyme inspired by crystal defect engineering.					
36907306	6	35	theme	photodynamic	1540:1551	arg1	activity					1464:1471	the enzyme catalytic activity	1443:1471	the enzyme catalytic activity	1443:1471	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	35	theme	photodynamic	1540:1551	arg1	PDT					1562:1564	PDT	1562:1564	PDT	1562:1564	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	35	theme	photodynamic	1540:1551	arg1	therapy					1553:1559	IR820-mediated photodynamic therapy	1525:1559	IR820-mediated photodynamic therapy (PDT)	1525:1565	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	1	36	theme	low	227:229	arg1	efficiency					231:240	low efficiency	227:240	low efficiency of commonly used metal catalysts	227:273	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	4	37	theme	redox	765:769	arg1	activity					771:778	redox activity	765:778	redox activity	765:778	The addition of Au determines the formation of oxygen vacancies, accelerates electron transfer, and enhances redox activity, thus significantly enhancing the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme.					
36907306	6	38	theme	oxygen	1622:1627	arg1	ROS					1638:1640	ROS	1638:1640	ROS	1638:1640	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	38	theme	oxygen	1622:1627	arg1	species					1629:1635	reactive oxygen species	1613:1635	reactive oxygen species (ROS) generation	1613:1652	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	5	39	theme	CaCO3	973:977	arg1	shell					979:983	a biomineralized CaCO3 shell	956:983	a biomineralized CaCO3 shell to avoid damage to normal tissues by the nanozyme while effectively encapsulating the photosensitizer IR820, and finally the tumor targeting ability of the nanoplatform was enhanced by the modification of hyaluronic acid	956:1204	Subsequently, we camouflaged the nanozyme using a biomineralized CaCO3 shell to avoid damage to normal tissues by the nanozyme while effectively encapsulating the photosensitizer IR820, and finally the tumor targeting ability of the nanoplatform was enhanced by the modification of hyaluronic acid.					
36907306	6	40	theme	Au	1256:1257	arg1	nanoplatform					1280:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	0	41	theme	catalytic	24:32	arg1	activity					34:41	Oxygen vacancy-enhanced catalytic activity	0:41	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen	0:115	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen species-augmented antitumor therapy.					
36907306	5	42	theme	biomineralized	958:971	arg1	shell					979:983	a biomineralized CaCO3 shell	956:983	a biomineralized CaCO3 shell to avoid damage to normal tissues by the nanozyme while effectively encapsulating the photosensitizer IR820, and finally the tumor targeting ability of the nanoplatform was enhanced by the modification of hyaluronic acid	956:1204	Subsequently, we camouflaged the nanozyme using a biomineralized CaCO3 shell to avoid damage to normal tissues by the nanozyme while effectively encapsulating the photosensitizer IR820, and finally the tumor targeting ability of the nanoplatform was enhanced by the modification of hyaluronic acid.					
36907306	3	43	theme	Au	598:599	arg1	nanozyme					607:614	Au@Co3O4 nanozyme	598:614	Au@Co3O4 nanozyme inspired by crystal defect engineering	598:653	In this work, we synthesized Au@Co3O4 nanozyme inspired by crystal defect engineering.					
36907306	2	44	theme	tumor	515:519	arg1	TME					539:541	TME	539:541	TME	539:541	For this purpose, we designed a composite nanoplatform capable of targeting tumors and selectively degrading in the tumor microenvironment (TME) to address these issues.					
36907306	2	44	theme	tumor	515:519	arg1	microenvironment					521:536	the tumor microenvironment	511:536	the tumor microenvironment (TME)	511:542	For this purpose, we designed a composite nanoplatform capable of targeting tumors and selectively degrading in the tumor microenvironment (TME) to address these issues.					
36907306	0	45	theme	hyaluronic	46:55	arg1	nanozyme					88:95	hyaluronic acid covered-biomineralization nanozyme	46:95	hyaluronic acid covered-biomineralization nanozyme for reactive oxygen	46:115	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen species-augmented antitumor therapy.					
36907306	6	46	theme	multimodal	1332:1341	arg1	imaging					1343:1349	multimodal imaging	1332:1349	multimodal imaging	1332:1349	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	4	47	theme	Au	672:673	arg1	addition					660:667	The addition	656:667	The addition of Au	656:673	The addition of Au determines the formation of oxygen vacancies, accelerates electron transfer, and enhances redox activity, thus significantly enhancing the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme.					
36907306	6	48	theme	chemodynamic	1494:1505	arg1	therapy					1507:1513	cobalt ion-mediated chemodynamic therapy	1474:1513	cobalt ion-mediated chemodynamic therapy (CDT)	1474:1519	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	48	theme	chemodynamic	1494:1505	arg1	activity					1464:1471	the enzyme catalytic activity	1443:1471	the enzyme catalytic activity	1443:1471	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	48	theme	chemodynamic	1494:1505	arg1	CDT					1516:1518	CDT	1516:1518	CDT	1516:1518	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	1	49	theme	unsatisfactory	344:357	arg1	efficacy					359:366	unsatisfactory efficacy	344:366	unsatisfactory efficacy of chemodynamic therapy alone	344:396	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	3	50	theme	crystal	628:634	arg1	engineering					643:653	crystal defect engineering	628:653	crystal defect engineering	628:653	In this work, we synthesized Au@Co3O4 nanozyme inspired by crystal defect engineering.					
36907306	6	51	theme	reactive	1613:1620	arg1	ROS					1638:1640	ROS	1638:1640	ROS	1638:1640	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	51	theme	reactive	1613:1620	arg1	species					1629:1635	reactive oxygen species	1613:1635	reactive oxygen species (ROS) generation	1613:1652	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	1	52	theme	used	254:257	arg1	catalysts					265:273	commonly used metal catalysts	245:273	commonly used metal catalysts	245:273	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	0	53	theme	covered-biomineralization	62:86	arg1	nanozyme					88:95	hyaluronic acid covered-biomineralization nanozyme	46:95	hyaluronic acid covered-biomineralization nanozyme for reactive oxygen	46:115	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen species-augmented antitumor therapy.					
36907306	5	54	theme	tumor	1110:1114	arg1	ability					1126:1132	the tumor targeting ability	1106:1132	the tumor targeting ability of the nanoplatform	1106:1152	Subsequently, we camouflaged the nanozyme using a biomineralized CaCO3 shell to avoid damage to normal tissues by the nanozyme while effectively encapsulating the photosensitizer IR820, and finally the tumor targeting ability of the nanoplatform was enhanced by the modification of hyaluronic acid.					
36907306	1	55	theme	Insufficient	154:165	arg1	content					185:191	Insufficient hydrogen peroxide content	154:191	Insufficient hydrogen peroxide content in tumor cells	154:206	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	0	56	theme	acid	57:60	arg1	nanozyme					88:95	hyaluronic acid covered-biomineralization nanozyme	46:95	hyaluronic acid covered-biomineralization nanozyme for reactive oxygen	46:115	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen species-augmented antitumor therapy.					
36907306	5	57	theme	nanoplatform	1141:1152	arg1	ability					1126:1132	the tumor targeting ability	1106:1132	the tumor targeting ability of the nanoplatform	1106:1152	Subsequently, we camouflaged the nanozyme using a biomineralized CaCO3 shell to avoid damage to normal tissues by the nanozyme while effectively encapsulating the photosensitizer IR820, and finally the tumor targeting ability of the nanoplatform was enhanced by the modification of hyaluronic acid.					
36907306	1	58	from	efficiency	231:240	arg1	cells					202:206	tumor cells	196:206	tumor cells	196:206	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	1	59	theme	hydrogen	167:174	arg1	peroxide					176:183	hydrogen peroxide	167:183	Insufficient hydrogen peroxide content in tumor cells	154:206	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	1	60	theme	chemodynamic	371:382	arg1	therapy					384:390	chemodynamic therapy	371:390	chemodynamic therapy alone	371:396	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	5	61	theme	photosensitizer	1071:1085	arg1	IR820					1087:1091	the photosensitizer IR820	1067:1091	the photosensitizer IR820	1067:1091	Subsequently, we camouflaged the nanozyme using a biomineralized CaCO3 shell to avoid damage to normal tissues by the nanozyme while effectively encapsulating the photosensitizer IR820, and finally the tumor targeting ability of the nanoplatform was enhanced by the modification of hyaluronic acid.					
36907306	5	62	theme	normal	1004:1009	arg1	tissues					1011:1017	normal tissues	1004:1017	normal tissues	1004:1017	Subsequently, we camouflaged the nanozyme using a biomineralized CaCO3 shell to avoid damage to normal tissues by the nanozyme while effectively encapsulating the photosensitizer IR820, and finally the tumor targeting ability of the nanoplatform was enhanced by the modification of hyaluronic acid.					
36907306	4	63	theme	-like	840:844	arg1	activities					880:889	the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities	810:889	the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme	810:905	The addition of Au determines the formation of oxygen vacancies, accelerates electron transfer, and enhances redox activity, thus significantly enhancing the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme.					
36907306	1	64	theme	peroxide	176:183	arg1	content					185:191	Insufficient hydrogen peroxide content	154:191	Insufficient hydrogen peroxide content in tumor cells	154:206	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	1	65	theme	metal	259:263	arg1	catalysts					265:273	commonly used metal catalysts	245:273	commonly used metal catalysts	245:273	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	6	66	theme	synergistic	1586:1596	arg1	enhancement					1598:1608	the synergistic enhancement	1582:1608	the synergistic enhancement of reactive oxygen species (ROS) generation	1582:1652	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	67	theme	light	1233:1237	arg1	NIR					1228:1230	NIR	1228:1230	NIR	1228:1230	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	67	theme	light	1233:1237	arg1	irradiation					1239:1249	near-infrared (NIR) light irradiation	1213:1249	near-infrared (NIR) light irradiation	1213:1249	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	68	dep	visualizes	1302:1311	arg1	only					1297:1300	only	1297:1300	only	1297:1300	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	4	69	theme	vacancies	710:718	arg1	formation					690:698	the formation	686:698	the formation of oxygen vacancies	686:718	The addition of Au determines the formation of oxygen vacancies, accelerates electron transfer, and enhances redox activity, thus significantly enhancing the superoxide dismutase (SOD)-like and catalase (CAT)-like catalytic activities of the nanozyme.					
36907306	1	70	theme	catalysts	265:273	arg1	content					185:191	Insufficient hydrogen peroxide content	154:191	Insufficient hydrogen peroxide content in tumor cells	154:206	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	1	70	theme	catalysts	265:273	arg1	pH					220:221	unsuitable pH	209:221	unsuitable pH	209:221	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	1	70	theme	catalysts	265:273	arg1	efficiency					231:240	low efficiency	227:240	low efficiency of commonly used metal catalysts	227:273	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	2	71	theme	capable	454:460	arg1	nanoplatform					441:452	a composite nanoplatform	429:452	a composite nanoplatform capable of targeting tumors and selectively degrading in the tumor microenvironment (TME)	429:542	For this purpose, we designed a composite nanoplatform capable of targeting tumors and selectively degrading in the tumor microenvironment (TME) to address these issues.					
36907306	3	72	theme	defect	636:641	arg1	engineering					643:653	crystal defect engineering	628:653	crystal defect engineering	628:653	In this work, we synthesized Au@Co3O4 nanozyme inspired by crystal defect engineering.					
36907306	6	73	theme	ion-mediated	1481:1492	arg1	therapy					1507:1513	cobalt ion-mediated chemodynamic therapy	1474:1513	cobalt ion-mediated chemodynamic therapy (CDT)	1474:1519	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	73	theme	ion-mediated	1481:1492	arg1	activity					1464:1471	the enzyme catalytic activity	1443:1471	the enzyme catalytic activity	1443:1471	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	73	theme	ion-mediated	1481:1492	arg1	CDT					1516:1518	CDT	1516:1518	CDT	1516:1518	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	74	theme	IR820-mediated	1525:1538	arg1	activity					1464:1471	the enzyme catalytic activity	1443:1471	the enzyme catalytic activity	1443:1471	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	74	theme	IR820-mediated	1525:1538	arg1	PDT					1562:1564	PDT	1562:1564	PDT	1562:1564	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	6	74	theme	IR820-mediated	1525:1538	arg1	therapy					1553:1559	IR820-mediated photodynamic therapy	1525:1559	IR820-mediated photodynamic therapy (PDT)	1525:1565	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
36907306	5	75	theme	acid	1201:1204	arg1	modification					1174:1185	the modification	1170:1185	the modification of hyaluronic acid	1170:1204	Subsequently, we camouflaged the nanozyme using a biomineralized CaCO3 shell to avoid damage to normal tissues by the nanozyme while effectively encapsulating the photosensitizer IR820, and finally the tumor targeting ability of the nanoplatform was enhanced by the modification of hyaluronic acid.					
36907306	0	76	theme	nanozyme	88:95	arg1	activity					34:41	Oxygen vacancy-enhanced catalytic activity	0:41	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen	0:115	Oxygen vacancy-enhanced catalytic activity of hyaluronic acid covered-biomineralization nanozyme for reactive oxygen species-augmented antitumor therapy.					
36907306	1	77	theme	therapy	384:390	arg1	efficacy					359:366	unsatisfactory efficacy	344:366	unsatisfactory efficacy of chemodynamic therapy alone	344:396	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	1	78	theme	tumor	196:200	arg1	cells					202:206	tumor cells	196:206	tumor cells	196:206	Insufficient hydrogen peroxide content in tumor cells, unsuitable pH and low efficiency of commonly used metal catalysts severely affect the efficiency of chemodynamic therapy, resulting in unsatisfactory efficacy of chemodynamic therapy alone.					
36907306	6	79	theme	HA	1277:1278	arg1	nanoplatform					1280:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	the Au@Co3O4@CaCO3/IR820@HA nanoplatform	1252:1291	Under near-infrared (NIR) light irradiation, the Au@Co3O4@CaCO3/IR820@HA nanoplatform not only visualizes the treatment with multimodal imaging, but also plays a photothermal sensitizing role through various strategies, while enhancing the enzyme catalytic activity, cobalt ion-mediated chemodynamic therapy (CDT) and IR820-mediated photodynamic therapy (PDT), and achieving the synergistic enhancement of reactive oxygen species (ROS) generation.					
35590970	0	0	theme	Acetate/Thermoplastic	77:97	arg1	Polyurethane					99:110	Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane	37:110	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	Design of a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor.					
35590970	4	1	theme	subtle	939:944	arg1	motions					952:958	subtle human motions	939:958	subtle human motions (such as finger bending, joint motion, etc.)	939:1003	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	4	2	theme	joint	985:989	arg1	such					961:964	such	961:964	such	961:964	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	4	2	theme	joint	985:989	arg1	motion					991:996	joint motion	985:996	joint motion	985:996	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	0	3	theme	Cellulose	67:75	arg1	Polyurethane					99:110	Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane	37:110	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	Design of a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor.					
35590970	1	4	theme	major	341:345	arg1	stability					296:304	the stability	292:304	the stability of micro-strain sensing	292:328	As a substitute for rigid sensors, flexible sensing materials have been greatly developed in recent years, but maintaining the stability of conductive fillers and the stability of micro-strain sensing is still a major challenge.					
35590970	1	4	theme	major	341:345	arg1	challenge					347:355	a major challenge	339:355	a major challenge	339:355	As a substitute for rigid sensors, flexible sensing materials have been greatly developed in recent years, but maintaining the stability of conductive fillers and the stability of micro-strain sensing is still a major challenge.					
35590970	1	5	theme	flexible	164:171	arg1	materials					181:189	flexible sensing materials	164:189	flexible sensing materials	164:189	As a substitute for rigid sensors, flexible sensing materials have been greatly developed in recent years, but maintaining the stability of conductive fillers and the stability of micro-strain sensing is still a major challenge.					
35590970	3	6	theme	%	830:830	arg1	strain					817:822	a very small strain	804:822	a very small strain of 0.5%	804:830	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	0	7	theme	Flexible	112:119	arg1	Sensor					121:126	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	Design of a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor.					
35590970	3	8	theme	nanofiber	685:693	arg1	membrane					695:702	the CA/TPU nanofiber membrane	674:702	the CA/TPU nanofiber membrane	674:702	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	4	9	dep	motions	952:958	arg1	such					961:964	such	961:964	such	961:964	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	4	9	dep	motions	952:958	arg1	motion					991:996	joint motion	985:996	joint motion	985:996	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	4	9	dep	motions	952:958	arg1	etc.					999:1002	etc.	999:1002	etc.	999:1002	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	3	10	theme	rGO	552:554	arg1	-as					603:605	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as	536:605	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%	536:830	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	3	11	theme	3.006	792:796	arg1	GF					786:787	a GF	784:787	a GF of 3.006	784:796	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	0	12	theme	Polyurethane	99:110	arg1	Sensor					121:126	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	Design of a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor.					
35590970	3	13	theme	impregnation	724:735	arg1	method					737:742	the ultrasonic impregnation method	709:742	the ultrasonic impregnation method	709:742	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	4	14	theme	wearable	1054:1061	arg1	devices					1074:1080	wearable electronic devices	1054:1080	wearable electronic devices	1054:1080	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	1	15	theme	conductive	269:278	arg1	fillers					280:286	conductive fillers	269:286	conductive fillers	269:286	As a substitute for rigid sensors, flexible sensing materials have been greatly developed in recent years, but maintaining the stability of conductive fillers and the stability of micro-strain sensing is still a major challenge.					
35590970	3	16	dep	-as	603:605	arg1	anchored					662:669	anchored	662:669	anchored	662:669	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	3	17	theme	oxide	545:549	arg1	-as					603:605	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as	536:605	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%	536:830	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	1	18	theme	sensing	173:179	arg1	materials					181:189	flexible sensing materials	164:189	flexible sensing materials	164:189	As a substitute for rigid sensors, flexible sensing materials have been greatly developed in recent years, but maintaining the stability of conductive fillers and the stability of micro-strain sensing is still a major challenge.					
35590970	0	19	theme	Sensor	121:126	arg1	Design					0:5	Design	0:5	Design of a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor.	0:127	Design of a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor.					
35590970	4	20	theme	motions	952:958	arg1	detection					926:934	the detection	922:934	the detection of subtle human motions (such as finger bending, joint motion, etc.)	922:1003	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	3	21	theme	rGO/GO	758:763	arg1	sensor					772:777	an rGO/GO@CA/TPU sensor	755:777	an rGO/GO@CA/TPU sensor	755:777	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	4	22	theme	finger	969:974	arg1	bending					976:982	finger bending	969:982	finger bending	969:982	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	3	23	theme	ultrasonic	713:722	arg1	method					737:742	the ultrasonic impregnation method	709:742	the ultrasonic impregnation method	709:742	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	3	24	theme	conductive	562:571	arg1	oxide					593:597	a conductive filler-and graphene oxide	560:597	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%	536:830	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	4	25	theme	electronic	1063:1072	arg1	devices					1074:1080	wearable electronic devices	1054:1080	wearable electronic devices	1054:1080	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	3	26	theme	CA/TPU	678:683	arg1	membrane					695:702	the CA/TPU nanofiber membrane	674:702	the CA/TPU nanofiber membrane	674:702	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	1	27	theme	fillers	280:286	arg1	stability					256:264	the stability	252:264	the stability of conductive fillers	252:286	As a substitute for rigid sensors, flexible sensing materials have been greatly developed in recent years, but maintaining the stability of conductive fillers and the stability of micro-strain sensing is still a major challenge.					
35590970	3	28	theme	filler-and	573:582	arg1	oxide					593:597	a conductive filler-and graphene oxide	560:597	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%	536:830	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	4	29	theme	high	869:872	arg1	sensitivity					874:884	its high sensitivity	865:884	its high sensitivity under extremely low strains	865:912	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	3	30	theme	-as	556:558	arg1	-as					603:605	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as	536:605	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%	536:830	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	2	31	theme	acetate	434:440	arg1	CA/TPU					462:467	CA/TPU	462:467	CA/TPU	462:467	In this experiment, we innovatively prepared a polyurethane-based cellulose acetate composite membrane (CA/TPU) with abundant mesopores through electrospinning.					
35590970	2	31	theme	acetate	434:440	arg1	membrane					452:459	a polyurethane-based cellulose acetate composite membrane	403:459	a polyurethane-based cellulose acetate composite membrane (CA/TPU)	403:468	In this experiment, we innovatively prepared a polyurethane-based cellulose acetate composite membrane (CA/TPU) with abundant mesopores through electrospinning.					
35590970	2	32	theme	composite	442:450	arg1	CA/TPU					462:467	CA/TPU	462:467	CA/TPU	462:467	In this experiment, we innovatively prepared a polyurethane-based cellulose acetate composite membrane (CA/TPU) with abundant mesopores through electrospinning.					
35590970	2	32	theme	composite	442:450	arg1	membrane					452:459	a polyurethane-based cellulose acetate composite membrane	403:459	a polyurethane-based cellulose acetate composite membrane (CA/TPU)	403:468	In this experiment, we innovatively prepared a polyurethane-based cellulose acetate composite membrane (CA/TPU) with abundant mesopores through electrospinning.					
35590970	2	33	theme	cellulose	424:432	arg1	CA/TPU					462:467	CA/TPU	462:467	CA/TPU	462:467	In this experiment, we innovatively prepared a polyurethane-based cellulose acetate composite membrane (CA/TPU) with abundant mesopores through electrospinning.					
35590970	2	33	theme	cellulose	424:432	arg1	membrane					452:459	a polyurethane-based cellulose acetate composite membrane	403:459	a polyurethane-based cellulose acetate composite membrane (CA/TPU)	403:468	In this experiment, we innovatively prepared a polyurethane-based cellulose acetate composite membrane (CA/TPU) with abundant mesopores through electrospinning.					
35590970	0	34	theme	Reduced	29:35	arg1	Sensor					121:126	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	Design of a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor.					
35590970	4	35	theme	potential	1029:1037	arg1	application					1039:1049	potential application	1029:1049	potential application in wearable electronic devices	1029:1080	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	2	36	theme	polyurethane-based	405:422	arg1	CA/TPU					462:467	CA/TPU	462:467	CA/TPU	462:467	In this experiment, we innovatively prepared a polyurethane-based cellulose acetate composite membrane (CA/TPU) with abundant mesopores through electrospinning.					
35590970	2	36	theme	polyurethane-based	405:422	arg1	membrane					452:459	a polyurethane-based cellulose acetate composite membrane	403:459	a polyurethane-based cellulose acetate composite membrane (CA/TPU)	403:468	In this experiment, we innovatively prepared a polyurethane-based cellulose acetate composite membrane (CA/TPU) with abundant mesopores through electrospinning.					
35590970	0	37	theme	Sensitive	19:27	arg1	Sensor					121:126	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	Design of a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor.					
35590970	3	38	theme	CA/TPU	765:770	arg1	sensor					772:777	an rGO/GO@CA/TPU sensor	755:777	an rGO/GO@CA/TPU sensor	755:777	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	2	39	theme	abundant	475:482	arg1	mesopores					484:492	abundant mesopores	475:492	abundant mesopores	475:492	In this experiment, we innovatively prepared a polyurethane-based cellulose acetate composite membrane (CA/TPU) with abundant mesopores through electrospinning.					
35590970	1	40	theme	recent	222:227	arg1	years					229:233	recent years	222:233	recent years	222:233	As a substitute for rigid sensors, flexible sensing materials have been greatly developed in recent years, but maintaining the stability of conductive fillers and the stability of micro-strain sensing is still a major challenge.					
35590970	0	41	theme	Oxide/Graphene	46:59	arg1	Polyurethane					99:110	Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane	37:110	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	Design of a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor.					
35590970	1	42	theme	micro-strain	309:320	arg1	sensing					322:328	micro-strain sensing	309:328	micro-strain sensing	309:328	As a substitute for rigid sensors, flexible sensing materials have been greatly developed in recent years, but maintaining the stability of conductive fillers and the stability of micro-strain sensing is still a major challenge.					
35590970	3	43	theme	graphene	584:591	arg1	oxide					593:597	a conductive filler-and graphene oxide	560:597	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%	536:830	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	0	44	theme	Graphene	37:44	arg1	Polyurethane					99:110	Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane	37:110	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	Design of a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor.					
35590970	1	45	theme	sensing	322:328	arg1	stability					296:304	the stability	292:304	the stability of micro-strain sensing	292:328	As a substitute for rigid sensors, flexible sensing materials have been greatly developed in recent years, but maintaining the stability of conductive fillers and the stability of micro-strain sensing is still a major challenge.					
35590970	1	45	theme	sensing	322:328	arg1	challenge					347:355	a major challenge	339:355	a major challenge	339:355	As a substitute for rigid sensors, flexible sensing materials have been greatly developed in recent years, but maintaining the stability of conductive fillers and the stability of micro-strain sensing is still a major challenge.					
35590970	3	46	theme	oxide	593:597	arg1	-as					603:605	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as	536:605	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%	536:830	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	3	47	theme	@	764:764	arg1	sensor					772:777	an rGO/GO@CA/TPU sensor	755:777	an rGO/GO@CA/TPU sensor	755:777	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	4	48	theme	film	856:859	arg1	sensitivity					874:884	its high sensitivity	865:884	its high sensitivity under extremely low strains	865:912	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	4	48	theme	film	856:859	arg1	flexibility					837:847	The flexibility	833:847	The flexibility of the film	833:859	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	3	49	theme	insulating	610:619	arg1	layer-which					621:631	an insulating layer-which	607:631	an insulating layer-which	607:631	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	3	50	theme	small	811:815	arg1	strain					817:822	a very small strain	804:822	a very small strain of 0.5%	804:830	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	0	51	theme	@	66:66	arg1	Polyurethane					99:110	Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane	37:110	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	Design of a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor.					
35590970	4	52	from	application	1039:1049	arg1	devices					1074:1080	wearable electronic devices	1054:1080	wearable electronic devices	1054:1080	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	1	53	theme	rigid	149:153	arg1	sensors					155:161	rigid sensors	149:161	rigid sensors	149:161	As a substitute for rigid sensors, flexible sensing materials have been greatly developed in recent years, but maintaining the stability of conductive fillers and the stability of micro-strain sensing is still a major challenge.					
35590970	3	54	dep	oxide	593:597	arg1	GO					600:601	GO	600:601	GO	600:601	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35590970	4	55	theme	low	902:904	arg1	strains					906:912	extremely low strains	892:912	extremely low strains	892:912	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	0	56	theme	Oxide	61:65	arg1	Polyurethane					99:110	Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane	37:110	a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor	10:126	Design of a Highly Sensitive Reduced Graphene Oxide/Graphene Oxide@Cellulose Acetate/Thermoplastic Polyurethane Flexible Sensor.					
35590970	4	57	theme	human	946:950	arg1	motions					952:958	subtle human motions	939:958	subtle human motions (such as finger bending, joint motion, etc.)	939:1003	The flexibility of the film and its high sensitivity under extremely low strains enables the detection of subtle human motions (such as finger bending, joint motion, etc.), making it suitable for potential application in wearable electronic devices.					
35590970	3	58	theme	graphene	536:543	arg1	-as					603:605	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as	536:605	graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%	536:830	Then, we reduced graphene oxide (rGO)-as a conductive filler-and graphene oxide (GO)-as an insulating layer-which were successively and firmly anchored on the CA/TPU nanofiber membrane with the ultrasonic impregnation method, to obtain an rGO/GO@CA/TPU sensor with a GF of 3.006 under a very small strain of 0.5%.					
35429939	0	0	theme	Edible	83:88	arg1	quality					90:96	Edible quality	83:96	Edible quality	83:96	Evaluation of partial salt-replacement with konjac glucomannan on chicken batters: Edible quality and physicochemical properties of heat-set gel.					
35429939	7	1	dep	indicated	1097:1105	arg1	reduced					1135:1141	reduced	1135:1141	reduced the excessively high viscosity of KGM	1135:1179	Compared with the batters only added with KGM, the relatively low heat-denatured temperature and storage modulus indicated appropriate salt supplement reduced the excessively high viscosity of KGM and accelerated the gel formation.					
35429939	7	1	dep	indicated	1097:1105	arg1	accelerated					1185:1195	accelerated	1185:1195	accelerated the gel formation	1185:1213	Compared with the batters only added with KGM, the relatively low heat-denatured temperature and storage modulus indicated appropriate salt supplement reduced the excessively high viscosity of KGM and accelerated the gel formation.					
35429939	5	2	theme	free	780:783	arg1	water					785:789	free water	780:789	free water	780:789	The relaxation properties illustrated salt provided KGM possibility for the conversion of free water to immobilized water.					
35429939	3	3	theme	loose	495:499	arg1	structure					501:509	loose structure	495:509	loose structure	495:509	The gels with low salt level manifested decreased hardness and loose structure due to the weak interaction of salt-soluble proteins.					
35429939	6	4	theme	sulfydryl	868:876	arg1	content					878:884	sulfydryl content	868:884	sulfydryl content	868:884	Additionally, the results of chemical interactions and sulfydryl content reflected KGM alleviated protein denaturation and the interaction of KGM-water was more dominant.					
35429939	5	5	theme	relaxation	694:703	arg1	properties					705:714	The relaxation properties	690:714	The relaxation properties illustrated salt	690:731	The relaxation properties illustrated salt provided KGM possibility for the conversion of free water to immobilized water.					
35429939	7	6	theme	gel	1201:1203	arg1	formation					1205:1213	the gel formation	1197:1213	the gel formation	1197:1213	Compared with the batters only added with KGM, the relatively low heat-denatured temperature and storage modulus indicated appropriate salt supplement reduced the excessively high viscosity of KGM and accelerated the gel formation.					
35429939	3	7	theme	weak	522:525	arg1	interaction					527:537	the weak interaction	518:537	the weak interaction of salt-soluble proteins	518:562	The gels with low salt level manifested decreased hardness and loose structure due to the weak interaction of salt-soluble proteins.					
35429939	3	8	theme	proteins	555:562	arg1	interaction					527:537	the weak interaction	518:537	the weak interaction of salt-soluble proteins	518:562	The gels with low salt level manifested decreased hardness and loose structure due to the weak interaction of salt-soluble proteins.					
35429939	7	9	theme	high	1159:1162	arg1	viscosity					1164:1172	the excessively high viscosity	1143:1172	the excessively high viscosity of KGM	1143:1179	Compared with the batters only added with KGM, the relatively low heat-denatured temperature and storage modulus indicated appropriate salt supplement reduced the excessively high viscosity of KGM and accelerated the gel formation.					
35429939	6	10	theme	protein	911:917	arg1	denaturation					919:930	protein denaturation	911:930	protein denaturation	911:930	Additionally, the results of chemical interactions and sulfydryl content reflected KGM alleviated protein denaturation and the interaction of KGM-water was more dominant.					
35429939	4	11	theme	network	617:623	arg1	formation					582:590	the formation	578:590	the formation of uniform composite gel network	578:623	CSK promoted the formation of uniform composite gel network by modifying moisture loss channels with the KGM-moisture phase.					
35429939	1	12	theme	gel	169:171	arg1	properties					173:182	gel properties	169:182	gel properties	169:182	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	0	13	theme	physicochemical	102:116	arg1	properties					118:127	physicochemical properties	102:127	physicochemical properties of heat-set gel	102:143	Evaluation of partial salt-replacement with konjac glucomannan on chicken batters: Edible quality and physicochemical properties of heat-set gel.					
35429939	7	14	theme	KGM	1177:1179	arg1	viscosity					1164:1172	the excessively high viscosity	1143:1172	the excessively high viscosity of KGM	1143:1179	Compared with the batters only added with KGM, the relatively low heat-denatured temperature and storage modulus indicated appropriate salt supplement reduced the excessively high viscosity of KGM and accelerated the gel formation.					
35429939	4	15	theme	loss	647:650	arg1	channels					652:659	moisture loss channels	638:659	moisture loss channels	638:659	CSK promoted the formation of uniform composite gel network by modifying moisture loss channels with the KGM-moisture phase.					
35429939	3	16	theme	low	446:448	arg1	level					455:459	low salt level	446:459	low salt level	446:459	The gels with low salt level manifested decreased hardness and loose structure due to the weak interaction of salt-soluble proteins.					
35429939	7	17	theme	salt	1119:1122	arg1	supplement					1124:1133	appropriate salt supplement	1107:1133	appropriate salt supplement	1107:1133	Compared with the batters only added with KGM, the relatively low heat-denatured temperature and storage modulus indicated appropriate salt supplement reduced the excessively high viscosity of KGM and accelerated the gel formation.					
35429939	1	18	dep	quality	157:163	arg1	The					146:148	The	146:148	The	146:148	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	1	19	theme	CSK	272:274	arg1	glucomannan					253:263	konjac glucomannan	246:263	konjac glucomannan (KGM) (CSK group)	246:281	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	1	19	theme	CSK	272:274	arg1	group					276:280	CSK group	272:280	CSK group	272:280	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	2	20	theme	CSK	297:299	arg1	group					301:305	CSK group	297:305	CSK group	297:305	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	1	21	theme	chicken	187:193	arg1	batter					195:200	chicken batter	187:200	chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group)	187:281	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	0	22	theme	salt-replacement	22:37	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of partial salt-replacement with konjac	0:49	Evaluation of partial salt-replacement with konjac glucomannan on chicken batters: Edible quality and physicochemical properties of heat-set gel.					
35429939	0	23	theme	heat-set	132:139	arg1	gel					141:143	heat-set gel	132:143	heat-set gel	132:143	Evaluation of partial salt-replacement with konjac glucomannan on chicken batters: Edible quality and physicochemical properties of heat-set gel.					
35429939	0	24	dep	batters	74:80	arg1	quality					90:96	Edible quality	83:96	Edible quality	83:96	Evaluation of partial salt-replacement with konjac glucomannan on chicken batters: Edible quality and physicochemical properties of heat-set gel.					
35429939	0	24	dep	batters	74:80	arg1	properties					118:127	physicochemical properties	102:127	physicochemical properties of heat-set gel	102:143	Evaluation of partial salt-replacement with konjac glucomannan on chicken batters: Edible quality and physicochemical properties of heat-set gel.					
35429939	1	25	theme	batter	195:200	arg1	quality					157:163	edible quality	150:163	edible quality	150:163	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	1	25	theme	batter	195:200	arg1	properties					173:182	gel properties	169:182	gel properties	169:182	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	0	26	theme	partial	14:20	arg1	salt-replacement					22:37	partial salt-replacement	14:37	partial salt-replacement with konjac	14:49	Evaluation of partial salt-replacement with konjac glucomannan on chicken batters: Edible quality and physicochemical properties of heat-set gel.					
35429939	7	27	theme	appropriate	1107:1117	arg1	supplement					1124:1133	appropriate salt supplement	1107:1133	appropriate salt supplement	1107:1133	Compared with the batters only added with KGM, the relatively low heat-denatured temperature and storage modulus indicated appropriate salt supplement reduced the excessively high viscosity of KGM and accelerated the gel formation.					
35429939	3	28	theme	salt-soluble	542:553	arg1	proteins					555:562	salt-soluble proteins	542:562	salt-soluble proteins	542:562	The gels with low salt level manifested decreased hardness and loose structure due to the weak interaction of salt-soluble proteins.					
35429939	3	29	theme	salt	450:453	arg1	level					455:459	low salt level	446:459	low salt level	446:459	The gels with low salt level manifested decreased hardness and loose structure due to the weak interaction of salt-soluble proteins.					
35429939	5	30	theme	water	785:789	arg1	conversion					766:775	the conversion	762:775	the conversion of free water to immobilized water	762:810	The relaxation properties illustrated salt provided KGM possibility for the conversion of free water to immobilized water.					
35429939	7	31	theme	storage	1081:1087	arg1	modulus					1089:1095	storage modulus	1081:1095	storage modulus	1081:1095	Compared with the batters only added with KGM, the relatively low heat-denatured temperature and storage modulus indicated appropriate salt supplement reduced the excessively high viscosity of KGM and accelerated the gel formation.					
35429939	6	32	theme	content	878:884	arg1	results					831:837	the results	827:837	the results of chemical interactions and sulfydryl content	827:884	Additionally, the results of chemical interactions and sulfydryl content reflected KGM alleviated protein denaturation and the interaction of KGM-water was more dominant.					
35429939	2	33	theme	gel	360:362	arg1	strength					364:371	similar gel strength	352:371	similar gel strength	352:371	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	4	34	theme	moisture	638:645	arg1	channels					652:659	moisture loss channels	638:659	moisture loss channels	638:659	CSK promoted the formation of uniform composite gel network by modifying moisture loss channels with the KGM-moisture phase.					
35429939	2	35	theme	color	377:381	arg1	distribution					383:394	color distribution	377:394	color distribution	377:394	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	0	36	theme	gel	141:143	arg1	quality					90:96	Edible quality	83:96	Edible quality	83:96	Evaluation of partial salt-replacement with konjac glucomannan on chicken batters: Edible quality and physicochemical properties of heat-set gel.					
35429939	0	36	theme	gel	141:143	arg1	properties					118:127	physicochemical properties	102:127	physicochemical properties of heat-set gel	102:143	Evaluation of partial salt-replacement with konjac glucomannan on chicken batters: Edible quality and physicochemical properties of heat-set gel.					
35429939	5	37	theme	KGM	742:744	arg1	possibility					746:756	KGM possibility	742:756	KGM possibility	742:756	The relaxation properties illustrated salt provided KGM possibility for the conversion of free water to immobilized water.					
35429939	4	38	theme	gel	613:615	arg1	network					617:623	uniform composite gel network	595:623	uniform composite gel network	595:623	CSK promoted the formation of uniform composite gel network by modifying moisture loss channels with the KGM-moisture phase.					
35429939	0	39	with	salt-replacement	22:37	arg1	konjac					44:49	konjac	44:49	konjac	44:49	Evaluation of partial salt-replacement with konjac glucomannan on chicken batters: Edible quality and physicochemical properties of heat-set gel.					
35429939	1	40	theme	partial	216:222	arg1	salt-replacement					224:239	partial salt-replacement	216:239	partial salt-replacement with konjac glucomannan (KGM) (CSK group)	216:281	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	5	41	theme	immobilized	794:804	arg1	water					806:810	immobilized water	794:810	immobilized water	794:810	The relaxation properties illustrated salt provided KGM possibility for the conversion of free water to immobilized water.					
35429939	2	42	theme	retention	328:336	arg1	capacity					338:345	higher water/fat retention capacity	311:345	higher water/fat retention capacity	311:345	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	3	43	with	gels	436:439	arg1	level					455:459	low salt level	446:459	low salt level	446:459	The gels with low salt level manifested decreased hardness and loose structure due to the weak interaction of salt-soluble proteins.					
35429939	6	44	theme	interactions	851:862	arg1	results					831:837	the results	827:837	the results of chemical interactions and sulfydryl content	827:884	Additionally, the results of chemical interactions and sulfydryl content reflected KGM alleviated protein denaturation and the interaction of KGM-water was more dominant.					
35429939	2	45	theme	water/fat	318:326	arg1	capacity					338:345	higher water/fat retention capacity	311:345	higher water/fat retention capacity	311:345	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	6	46	theme	KGM-water	955:963	arg1	dominant					974:981	dominant	974:981	dominant	974:981	Additionally, the results of chemical interactions and sulfydryl content reflected KGM alleviated protein denaturation and the interaction of KGM-water was more dominant.					
35429939	6	46	theme	KGM-water	955:963	arg1	denaturation					919:930	protein denaturation	911:930	protein denaturation	911:930	Additionally, the results of chemical interactions and sulfydryl content reflected KGM alleviated protein denaturation and the interaction of KGM-water was more dominant.					
35429939	6	46	theme	KGM-water	955:963	arg1	interaction					940:950	the interaction	936:950	the interaction of KGM-water	936:963	Additionally, the results of chemical interactions and sulfydryl content reflected KGM alleviated protein denaturation and the interaction of KGM-water was more dominant.					
35429939	7	47	theme	low	1046:1048	arg1	temperature					1065:1075	the relatively low heat-denatured temperature	1031:1075	the relatively low heat-denatured temperature	1031:1075	Compared with the batters only added with KGM, the relatively low heat-denatured temperature and storage modulus indicated appropriate salt supplement reduced the excessively high viscosity of KGM and accelerated the gel formation.					
35429939	6	48	theme	chemical	842:849	arg1	interactions					851:862	chemical interactions	842:862	chemical interactions	842:862	Additionally, the results of chemical interactions and sulfydryl content reflected KGM alleviated protein denaturation and the interaction of KGM-water was more dominant.					
35429939	2	49	theme	higher	311:316	arg1	capacity					338:345	higher water/fat retention capacity	311:345	higher water/fat retention capacity	311:345	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	4	50	theme	uniform	595:601	arg1	network					617:623	uniform composite gel network	595:623	uniform composite gel network	595:623	CSK promoted the formation of uniform composite gel network by modifying moisture loss channels with the KGM-moisture phase.					
35429939	7	51	theme	heat-denatured	1050:1063	arg1	temperature					1065:1075	the relatively low heat-denatured temperature	1031:1075	the relatively low heat-denatured temperature	1031:1075	Compared with the batters only added with KGM, the relatively low heat-denatured temperature and storage modulus indicated appropriate salt supplement reduced the excessively high viscosity of KGM and accelerated the gel formation.					
35429939	4	52	theme	KGM-moisture	670:681	arg1	phase					683:687	the KGM-moisture phase	666:687	the KGM-moisture phase	666:687	CSK promoted the formation of uniform composite gel network by modifying moisture loss channels with the KGM-moisture phase.					
35429939	2	53	theme	normal	410:415	arg1	addition					422:429	normal salt addition	410:429	normal salt addition	410:429	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	2	54	theme	similar	352:358	arg1	strength					364:371	similar gel strength	352:371	similar gel strength	352:371	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	1	55	theme	konjac	246:251	arg1	KGM					266:268	KGM	266:268	KGM	266:268	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	1	55	theme	konjac	246:251	arg1	glucomannan					253:263	konjac glucomannan	246:263	konjac glucomannan (KGM) (CSK group)	246:281	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	1	55	theme	konjac	246:251	arg1	group					276:280	CSK group	272:280	CSK group	272:280	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	0	56	theme	chicken	66:72	arg1	batters					74:80	chicken batters	66:80	chicken batters: Edible quality and physicochemical properties of heat-set gel	66:143	Evaluation of partial salt-replacement with konjac glucomannan on chicken batters: Edible quality and physicochemical properties of heat-set gel.					
35429939	2	57	contain	had	307:309	arg2	distribution					383:394	color distribution	377:394	color distribution	377:394	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	2	57	contain	had	307:309	arg2	capacity					338:345	higher water/fat retention capacity	311:345	higher water/fat retention capacity	311:345	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	2	57	contain	had	307:309	arg2	strength					364:371	similar gel strength	352:371	similar gel strength	352:371	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	2	57	contain	had	307:309	arg1	group					301:305	CSK group	297:305	CSK group	297:305	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	2	58	theme	salt	417:420	arg1	addition					422:429	normal salt addition	410:429	normal salt addition	410:429	CSK group had higher water/fat retention capacity, but similar gel strength and color distribution compared with normal salt addition.					
35429939	1	59	with	salt-replacement	224:239	arg1	KGM					266:268	KGM	266:268	KGM	266:268	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	1	59	with	salt-replacement	224:239	arg1	glucomannan					253:263	konjac glucomannan	246:263	konjac glucomannan (KGM) (CSK group)	246:281	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	1	59	with	salt-replacement	224:239	arg1	group					276:280	CSK group	272:280	CSK group	272:280	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	1	60	theme	edible	150:155	arg1	quality					157:163	edible quality	150:163	edible quality	150:163	The edible quality and gel properties of chicken batter influenced by partial salt-replacement with konjac glucomannan (KGM) (CSK group) were studied.					
35429939	4	61	theme	composite	603:611	arg1	network					617:623	uniform composite gel network	595:623	uniform composite gel network	595:623	CSK promoted the formation of uniform composite gel network by modifying moisture loss channels with the KGM-moisture phase.					
36930680	4	0	theme	p-value	974:980	arg1	plasma					966:971	human plasma	960:971	human plasma (p-value 0.0934)	960:988	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	4	0	theme	p-value	974:980	arg1	0.0934					982:987	p-value 0.0934	974:987	p-value 0.0934	974:987	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	6	1	theme	relevant	1168:1175	arg1	approach					1177:1184	a clinically relevant approach	1155:1184	a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors	1155:1294	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	2	2	theme	aureus	370:375	arg1	activity					355:362	the binding activity	343:362	the binding activity of S. aureus to Seraph affinity filters based on heparin coated beads	343:432	OBJECTIVES The aim of this study was to analyse the binding activity of S. aureus to Seraph affinity filters based on heparin coated beads and to identify effectors influencing this binding activity.					
36930680	5	3	theme	SaeS	1093:1096	arg1	loss					1064:1067	the loss	1060:1067	the loss of SarA, SigB, Lgt, and SaeS	1060:1096	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS had no significant influence on its binding activity.					
36930680	4	4	theme	0.9	860:862	arg1	%					863:863	%	863:863	%	863:863	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	3	5	theme	Newman	566:571	arg1	strains					573:579	gfp-expressing S. aureus Newman strains	541:579	gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma	541:629	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	10	6	theme	human	1918:1922	arg1	plasma					1930:1935	human blood plasma	1918:1935	human blood plasma	1918:1935	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	3	7	theme	Seraph	716:721	arg1	filters					732:738	Seraph affinity filters	716:738	Seraph affinity filters	716:738	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	4	8	theme	%	842:842	arg1	removal					829:835	a percent removal	819:835	a percent removal of 42%	819:842	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	6	9	theme	reduction	1384:1392	arg1	rate					1366:1369	the rate	1362:1369	the rate of bacterial reduction	1362:1392	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	7	10	theme	statistical	1508:1518	arg1	significance					1520:1531	statistical significance	1508:1531	statistical significance	1508:1531	Within the first four hours, the number of bacteria decreased continuously and the reduction in bacteria reached statistical significance after two hours of treatment (percentage reduction 64%, p-value 0.01165).					
36930680	0	11	theme	treatment	115:123	arg1	duration					125:132	treatment duration	115:132	treatment duration	115:132	Staphylococcus aureus binding to Seraph® 100 Microbind® Affinity Filter: Effects of surface protein expression and treatment duration.					
36930680	4	12	theme	Seraph	893:898	arg1	columns					905:911	Seraph mini columns	893:911	Seraph mini columns	893:911	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	10	13	theme	Seraph	1808:1813	arg1	filter					1824:1829	The Seraph affinity filter	1804:1829	The Seraph affinity filter	1804:1829	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	10	13	theme	Seraph	1808:1813	arg1	method					1885:1890	a highly efficient method	1866:1890	a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration	1866:2013	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	6	14	theme	Microbind®	1208:1217	arg1	Filter					1228:1233	the Seraph® 100 Microbind® Affinity Filter	1192:1233	the Seraph® 100 Microbind® Affinity Filter	1192:1233	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	8	15	theme	final	1611:1615	arg1	reduction					1617:1625	The final reduction	1607:1625	The final reduction after four hours of treatment	1607:1655	The final reduction after four hours of treatment was close to 90% and is dependent on donor.					
36930680	8	15	theme	final	1611:1615	arg1	%					1672:1672	close to 90%	1661:1672	close to 90%	1661:1672	The final reduction after four hours of treatment was close to 90% and is dependent on donor.					
36930680	8	15	theme	final	1611:1615	arg1	dependent					1681:1689	dependent	1681:1689	dependent	1681:1689	The final reduction after four hours of treatment was close to 90% and is dependent on donor.					
36930680	1	16	theme	blood	241:245	arg1	infections					254:263	blood stream infections	241:263	blood stream infections	241:263	INTRODUCTION Extracorporeal blood purification systems represent a promising alternative for treatment of blood stream infections with multiresistant bacteria.					
36930680	10	17	theme	blood	1967:1971	arg1	composition					1980:1990	blood plasma composition	1967:1990	blood plasma composition	1967:1990	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	6	18	theme	Seraph®	1196:1202	arg1	Filter					1228:1233	the Seraph® 100 Microbind® Affinity Filter	1192:1233	the Seraph® 100 Microbind® Affinity Filter	1192:1233	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	6	19	from	donors	1289:1294	arg1	plasma					1262:1267	human blood plasma	1250:1267	human blood plasma from four different donors	1250:1294	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	6	19	from	donors	1289:1294	arg1	Filter					1228:1233	the Seraph® 100 Microbind® Affinity Filter	1192:1233	the Seraph® 100 Microbind® Affinity Filter	1192:1233	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	6	19	from	donors	1289:1294	arg1	ml					1244:1245	1000 ml	1239:1245	1000 ml of human blood plasma from four different donors	1239:1294	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	3	20	theme	%	605:605	arg1	NaCl					607:610	0.9% NaCl	602:610	0.9% NaCl	602:610	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	10	21	theme	dependent	1954:1962	arg1	efficiency					1943:1952	efficiency	1943:1952	efficiency dependent on blood plasma composition and treatment duration	1943:2013	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	3	22	theme	aureus	559:564	arg1	strains					573:579	gfp-expressing S. aureus Newman strains	541:579	gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma	541:629	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	1	23	theme	INTRODUCTION	135:146	arg1	systems					182:188	INTRODUCTION Extracorporeal blood purification systems	135:188	INTRODUCTION Extracorporeal blood purification systems	135:188	INTRODUCTION Extracorporeal blood purification systems represent a promising alternative for treatment of blood stream infections with multiresistant bacteria.					
36930680	10	24	theme	treatment	1996:2004	arg1	duration					2006:2013	treatment duration	1996:2013	treatment duration	1996:2013	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	3	25	theme	blood	618:622	arg1	plasma					624:629	blood plasma	618:629	blood plasma	618:629	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	8	26	theme	treatment	1647:1655	arg1	hours					1638:1642	four hours	1633:1642	four hours of treatment	1633:1655	The final reduction after four hours of treatment was close to 90% and is dependent on donor.					
36930680	1	27	theme	blood	163:167	arg1	systems					182:188	INTRODUCTION Extracorporeal blood purification systems	135:188	INTRODUCTION Extracorporeal blood purification systems	135:188	INTRODUCTION Extracorporeal blood purification systems represent a promising alternative for treatment of blood stream infections with multiresistant bacteria.					
36930680	6	28	theme	blood	1256:1260	arg1	plasma					1262:1267	human blood plasma	1250:1267	human blood plasma from four different donors	1250:1294	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	7	29	dep	reached	1500:1506	arg1	%					1586:1586	percentage reduction 64%	1563:1586	percentage reduction 64%	1563:1586	Within the first four hours, the number of bacteria decreased continuously and the reduction in bacteria reached statistical significance after two hours of treatment (percentage reduction 64%, p-value 0.01165).					
36930680	7	29	dep	reached	1500:1506	arg1	p-value					1589:1595	p-value 0.01165	1589:1603	p-value 0.01165	1589:1603	Within the first four hours, the number of bacteria decreased continuously and the reduction in bacteria reached statistical significance after two hours of treatment (percentage reduction 64%, p-value 0.01165).					
36930680	7	30	theme	reduction	1574:1582	arg1	%					1586:1586	percentage reduction 64%	1563:1586	percentage reduction 64%	1563:1586	Within the first four hours, the number of bacteria decreased continuously and the reduction in bacteria reached statistical significance after two hours of treatment (percentage reduction 64%, p-value 0.01165).					
36930680	7	30	theme	reduction	1574:1582	arg1	p-value					1589:1595	p-value 0.01165	1589:1603	p-value 0.01165	1589:1603	Within the first four hours, the number of bacteria decreased continuously and the reduction in bacteria reached statistical significance after two hours of treatment (percentage reduction 64%, p-value 0.01165).					
36930680	4	31	theme	aureus	768:773	arg1	activity					753:760	The binding activity	741:760	The binding activity of S. aureus	741:773	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	10	32	theme	heparin-coated	1841:1854	arg1	beads					1856:1860	heparin-coated beads	1841:1860	heparin-coated beads	1841:1860	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	0	33	theme	surface	84:90	arg1	expression					100:109	surface protein expression	84:109	surface protein expression	84:109	Staphylococcus aureus binding to Seraph® 100 Microbind® Affinity Filter: Effects of surface protein expression and treatment duration.					
36930680	5	34	theme	surface	1020:1026	arg1	proteins					1028:1035	surface proteins	1020:1035	surface proteins	1020:1035	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS had no significant influence on its binding activity.					
36930680	7	35	from	reduction	1478:1486	arg1	bacteria					1491:1498	bacteria	1491:1498	bacteria	1491:1498	Within the first four hours, the number of bacteria decreased continuously and the reduction in bacteria reached statistical significance after two hours of treatment (percentage reduction 64%, p-value 0.01165).					
36930680	2	36	theme	heparin	413:419	arg1	beads					428:432	heparin coated beads	413:432	heparin coated beads	413:432	OBJECTIVES The aim of this study was to analyse the binding activity of S. aureus to Seraph affinity filters based on heparin coated beads and to identify effectors influencing this binding activity.					
36930680	0	37	theme	expression	100:109	arg1	Effects					73:79	Effects	73:79	Effects of surface protein expression and treatment duration	73:132	Staphylococcus aureus binding to Seraph® 100 Microbind® Affinity Filter: Effects of surface protein expression and treatment duration.					
36930680	4	38	theme	human	960:964	arg1	plasma					966:971	human plasma	960:971	human plasma (p-value 0.0934)	960:988	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	4	38	theme	human	960:964	arg1	0.0934					982:987	p-value 0.0934	974:987	p-value 0.0934	974:987	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	4	39	theme	binding	745:751	arg1	activity					753:760	The binding activity	741:760	The binding activity of S. aureus	741:773	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	6	40	theme	treatment	1313:1321	arg1	duration					1301:1308	the duration	1297:1308	the duration of treatment	1297:1321	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	5	41	theme	SigB	1078:1081	arg1	loss					1064:1067	the loss	1060:1067	the loss of SarA, SigB, Lgt, and SaeS	1060:1096	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS had no significant influence on its binding activity.					
36930680	5	42	theme	Lgt	1084:1086	arg1	loss					1064:1067	the loss	1060:1067	the loss of SarA, SigB, Lgt, and SaeS	1060:1096	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS had no significant influence on its binding activity.					
36930680	4	43	theme	%	942:942	arg1	removal					924:930	a percent removal	914:930	a percent removal of only 10%	914:942	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	0	44	theme	Seraph®	33:39	arg1	Filter					65:70	Seraph® 100 Microbind® Affinity Filter	33:70	Seraph® 100 Microbind® Affinity Filter	33:70	Staphylococcus aureus binding to Seraph® 100 Microbind® Affinity Filter: Effects of surface protein expression and treatment duration.					
36930680	1	45	with	treatment	228:236	arg1	bacteria					285:292	multiresistant bacteria	270:292	multiresistant bacteria	270:292	INTRODUCTION Extracorporeal blood purification systems represent a promising alternative for treatment of blood stream infections with multiresistant bacteria.					
36930680	4	46	theme	percent	821:827	arg1	removal					829:835	a percent removal	819:835	a percent removal of 42%	819:842	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	0	47	theme	Microbind®	45:54	arg1	Filter					65:70	Seraph® 100 Microbind® Affinity Filter	33:70	Seraph® 100 Microbind® Affinity Filter	33:70	Staphylococcus aureus binding to Seraph® 100 Microbind® Affinity Filter: Effects of surface protein expression and treatment duration.					
36930680	5	48	theme	SarA	1072:1075	arg1	loss					1064:1067	the loss	1060:1067	the loss of SarA, SigB, Lgt, and SaeS	1060:1096	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS had no significant influence on its binding activity.					
36930680	10	49	theme	efficient	1875:1883	arg1	filter					1824:1829	The Seraph affinity filter	1804:1829	The Seraph affinity filter	1804:1829	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	10	49	theme	efficient	1875:1883	arg1	method					1885:1890	a highly efficient method	1866:1890	a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration	1866:2013	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	3	50	theme	FACS	680:683	arg1	analyses					685:692	FACS analyses	680:692	FACS analyses	680:692	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	5	51	theme	significant	1105:1115	arg1	influence					1117:1125	no significant influence	1102:1125	no significant influence	1102:1125	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS had no significant influence on its binding activity.					
36930680	0	52	dep	binding	22:28	arg1	Effects					73:79	Effects	73:79	Effects of surface protein expression and treatment duration	73:132	Staphylococcus aureus binding to Seraph® 100 Microbind® Affinity Filter: Effects of surface protein expression and treatment duration.					
36930680	3	53	theme	bacteria	668:675	arg1	number					650:655	the number	646:655	the number of unbound bacteria	646:675	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	3	54	used	used	536:539	arg2	we					533:534	we	533:534	we	533:534	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	2	55	theme	affinity	387:394	arg1	filters					396:402	Seraph affinity filters	380:402	Seraph affinity filters based on heparin coated beads	380:432	OBJECTIVES The aim of this study was to analyse the binding activity of S. aureus to Seraph affinity filters based on heparin coated beads and to identify effectors influencing this binding activity.					
36930680	6	56	theme	critical	1343:1350	arg1	effect					1352:1357	a critical effect	1341:1357	a critical effect	1341:1357	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	9	57	theme	Seraph®	1717:1723	arg1	cells					1785:1789	approximately 5 x 108 cells	1763:1789	approximately 5 x 108 cells	1763:1789	The capacity of Seraph® 100 for S. aureus in human plasma was approximately 5 x 108 cells.					
36930680	9	57	theme	Seraph®	1717:1723	arg1	capacity					1705:1712	The capacity	1701:1712	The capacity of Seraph® 100 for S. aureus in human plasma	1701:1757	The capacity of Seraph® 100 for S. aureus in human plasma was approximately 5 x 108 cells.					
36930680	2	58	theme	study	322:326	arg1	aim					310:312	The aim	306:312	The aim of this study	306:326	OBJECTIVES The aim of this study was to analyse the binding activity of S. aureus to Seraph affinity filters based on heparin coated beads and to identify effectors influencing this binding activity.					
36930680	10	59	theme	blood	1924:1928	arg1	plasma					1930:1935	human blood plasma	1918:1935	human blood plasma	1918:1935	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	3	60	theme	gfp-expressing	541:554	arg1	strains					573:579	gfp-expressing S. aureus Newman strains	541:579	gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma	541:629	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	6	61	theme	different	1279:1287	arg1	donors					1289:1294	four different donors	1274:1294	four different donors	1274:1294	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	5	62	contain	had	1098:1100	arg2	influence					1117:1125	no significant influence	1102:1125	no significant influence	1102:1125	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS had no significant influence on its binding activity.					
36930680	5	62	contain	had	1098:1100	arg1	composition					1005:1015	The different composition	991:1015	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS	991:1096	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS had no significant influence on its binding activity.					
36930680	3	63	theme	affinity	723:730	arg1	filters					732:738	Seraph affinity filters	716:738	Seraph affinity filters	716:738	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	1	64	theme	promising	202:210	arg1	alternative					212:222	a promising alternative	200:222	a promising alternative for treatment of blood stream infections with multiresistant bacteria	200:292	INTRODUCTION Extracorporeal blood purification systems represent a promising alternative for treatment of blood stream infections with multiresistant bacteria.					
36930680	8	65	dep	90	1670:1671	arg1	to					1667:1668	to	1667:1668	to	1667:1668	The final reduction after four hours of treatment was close to 90% and is dependent on donor.					
36930680	2	66	dep	OBJECTIVES	295:304	arg1	was					328:330	was	328:330	was to analyse the binding activity of S. aureus to Seraph affinity filters based on heparin coated beads and to identify effectors influencing this binding activity	328:492	OBJECTIVES The aim of this study was to analyse the binding activity of S. aureus to Seraph affinity filters based on heparin coated beads and to identify effectors influencing this binding activity.					
36930680	0	67	theme	duration	125:132	arg1	Effects					73:79	Effects	73:79	Effects of surface protein expression and treatment duration	73:132	Staphylococcus aureus binding to Seraph® 100 Microbind® Affinity Filter: Effects of surface protein expression and treatment duration.					
36930680	6	68	theme	bacterial	1374:1382	arg1	reduction					1384:1392	bacterial reduction	1374:1392	bacterial reduction	1374:1392	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	9	69	theme	human	1746:1750	arg1	plasma					1752:1757	human plasma	1746:1757	human plasma	1746:1757	The capacity of Seraph® 100 for S. aureus in human plasma was approximately 5 x 108 cells.					
36930680	6	70	theme	Affinity	1219:1226	arg1	Filter					1228:1233	the Seraph® 100 Microbind® Affinity Filter	1192:1233	the Seraph® 100 Microbind® Affinity Filter	1192:1233	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	5	71	theme	different	995:1003	arg1	composition					1005:1015	The different composition	991:1015	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS	991:1096	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS had no significant influence on its binding activity.					
36930680	10	72	theme	plasma	1973:1978	arg1	composition					1980:1990	blood plasma composition	1967:1990	blood plasma composition	1967:1990	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	5	73	from	composition	1005:1015	arg1	aureus					1043:1048	S. aureus	1040:1048	S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS	1040:1096	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS had no significant influence on its binding activity.					
36930680	6	74	theme	human	1250:1254	arg1	plasma					1262:1267	human blood plasma	1250:1267	human blood plasma from four different donors	1250:1294	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	3	75	theme	0.9	602:604	arg1	%					605:605	%	605:605	%	605:605	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	4	76	theme	%	863:863	arg1	p-value					871:877	p-value 0.0472	871:884	p-value 0.0472	871:884	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	4	76	theme	%	863:863	arg1	NaCl					865:868	0.9% NaCl	860:868	0.9% NaCl (p-value 0.0472) using Seraph mini columns	860:911	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	7	77	theme	bacteria	1438:1445	arg1	number					1428:1433	the number	1424:1433	the number of bacteria	1424:1445	Within the first four hours, the number of bacteria decreased continuously and the reduction in bacteria reached statistical significance after two hours of treatment (percentage reduction 64%, p-value 0.01165).					
36930680	1	78	theme	Extracorporeal	148:161	arg1	systems					182:188	INTRODUCTION Extracorporeal blood purification systems	135:188	INTRODUCTION Extracorporeal blood purification systems	135:188	INTRODUCTION Extracorporeal blood purification systems represent a promising alternative for treatment of blood stream infections with multiresistant bacteria.					
36930680	4	79	theme	percent	916:922	arg1	removal					924:930	a percent removal	914:930	a percent removal of only 10%	914:942	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	10	80	dep	CONCLUSIONS	1792:1802	arg1	filter					1824:1829	The Seraph affinity filter	1804:1829	The Seraph affinity filter	1804:1829	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	10	80	dep	CONCLUSIONS	1792:1802	arg1	method					1885:1890	a highly efficient method	1866:1890	a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration	1866:2013	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	1	81	theme	stream	247:252	arg1	infections					254:263	blood stream infections	241:263	blood stream infections	241:263	INTRODUCTION Extracorporeal blood purification systems represent a promising alternative for treatment of blood stream infections with multiresistant bacteria.					
36930680	5	82	theme	binding	1134:1140	arg1	activity					1142:1149	its binding activity	1130:1149	its binding activity	1130:1149	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS had no significant influence on its binding activity.					
36930680	10	83	theme	affinity	1815:1822	arg1	filter					1824:1829	The Seraph affinity filter	1804:1829	The Seraph affinity filter	1804:1829	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	10	83	theme	affinity	1815:1822	arg1	method					1885:1890	a highly efficient method	1866:1890	a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration	1866:2013	CONCLUSIONS The Seraph affinity filter, based on heparin-coated beads, is a highly efficient method for reducing S. aureus in human blood plasma, with efficiency dependent on blood plasma composition and treatment duration.					
36930680	6	84	theme	plasma	1262:1267	arg1	Filter					1228:1233	the Seraph® 100 Microbind® Affinity Filter	1192:1233	the Seraph® 100 Microbind® Affinity Filter	1192:1233	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	6	84	theme	plasma	1262:1267	arg1	ml					1244:1245	1000 ml	1239:1245	1000 ml of human blood plasma from four different donors	1239:1294	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	1	85	theme	purification	169:180	arg1	systems					182:188	INTRODUCTION Extracorporeal blood purification systems	135:188	INTRODUCTION Extracorporeal blood purification systems	135:188	INTRODUCTION Extracorporeal blood purification systems represent a promising alternative for treatment of blood stream infections with multiresistant bacteria.					
36930680	6	86	from	ml	1244:1245	arg1	donors					1289:1294	four different donors	1274:1294	four different donors	1274:1294	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	0	87	theme	protein	92:98	arg1	expression					100:109	surface protein expression	84:109	surface protein expression	84:109	Staphylococcus aureus binding to Seraph® 100 Microbind® Affinity Filter: Effects of surface protein expression and treatment duration.					
36930680	4	88	theme	mini	900:903	arg1	columns					905:911	Seraph mini columns	893:911	Seraph mini columns	893:911	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	5	89	theme	proteins	1028:1035	arg1	composition					1005:1015	The different composition	991:1015	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS	991:1096	The different composition of surface proteins in S. aureus caused by the loss of SarA, SigB, Lgt, and SaeS had no significant influence on its binding activity.					
36930680	7	90	theme	percentage	1563:1572	arg1	%					1586:1586	percentage reduction 64%	1563:1586	percentage reduction 64%	1563:1586	Within the first four hours, the number of bacteria decreased continuously and the reduction in bacteria reached statistical significance after two hours of treatment (percentage reduction 64%, p-value 0.01165).					
36930680	7	90	theme	percentage	1563:1572	arg1	p-value					1589:1595	p-value 0.01165	1589:1603	p-value 0.01165	1589:1603	Within the first four hours, the number of bacteria decreased continuously and the reduction in bacteria reached statistical significance after two hours of treatment (percentage reduction 64%, p-value 0.01165).					
36930680	7	91	theme	treatment	1552:1560	arg1	hours					1543:1547	two hours	1539:1547	two hours of treatment	1539:1560	Within the first four hours, the number of bacteria decreased continuously and the reduction in bacteria reached statistical significance after two hours of treatment (percentage reduction 64%, p-value 0.01165).					
36930680	6	92	from	Filter	1228:1233	arg1	donors					1289:1294	four different donors	1274:1294	four different donors	1274:1294	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	7	93	theme	first	1406:1410	arg1	hours					1417:1421	the first four hours	1402:1421	the first four hours	1402:1421	Within the first four hours, the number of bacteria decreased continuously and the reduction in bacteria reached statistical significance after two hours of treatment (percentage reduction 64%, p-value 0.01165).					
36930680	2	94	theme	coated	421:426	arg1	beads					428:432	heparin coated beads	413:432	heparin coated beads	413:432	OBJECTIVES The aim of this study was to analyse the binding activity of S. aureus to Seraph affinity filters based on heparin coated beads and to identify effectors influencing this binding activity.					
36930680	0	95	theme	Affinity	56:63	arg1	Filter					65:70	Seraph® 100 Microbind® Affinity Filter	33:70	Seraph® 100 Microbind® Affinity Filter	33:70	Staphylococcus aureus binding to Seraph® 100 Microbind® Affinity Filter: Effects of surface protein expression and treatment duration.					
36930680	2	96	theme	binding	477:483	arg1	activity					485:492	this binding activity	472:492	this binding activity	472:492	OBJECTIVES The aim of this study was to analyse the binding activity of S. aureus to Seraph affinity filters based on heparin coated beads and to identify effectors influencing this binding activity.					
36930680	3	97	theme	binding	515:521	arg1	activity					523:530	the binding activity	511:530	the binding activity	511:530	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	6	98	contain	have	1336:1339	arg2	effect					1352:1357	a critical effect	1341:1357	a critical effect	1341:1357	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	6	98	contain	have	1336:1339	arg1	duration					1301:1308	the duration	1297:1308	the duration of treatment	1297:1321	In a clinically relevant approach using the Seraph® 100 Microbind® Affinity Filter and 1000 ml of human blood plasma from four different donors, the duration of treatment was shown to have a critical effect on the rate of bacterial reduction.					
36930680	4	99	located	observed	848:855	arg2	removal					829:835	a percent removal	819:835	a percent removal of 42%	819:842	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	4	99	located	observed	848:855	arg1	p-value					871:877	p-value 0.0472	871:884	p-value 0.0472	871:884	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	4	99	located	observed	848:855	arg1	NaCl					865:868	0.9% NaCl	860:868	0.9% NaCl (p-value 0.0472) using Seraph mini columns	860:911	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	3	100	theme	unbound	660:666	arg1	bacteria					668:675	unbound bacteria	660:675	unbound bacteria	660:675	RESULTS To test the binding activity, we used gfp-expressing S. aureus Newman strains inoculated either in 0.9% NaCl or in blood plasma and determined the number of unbound bacteria by FACS analyses after passing through Seraph affinity filters.					
36930680	2	101	theme	binding	347:353	arg1	activity					355:362	the binding activity	343:362	the binding activity of S. aureus to Seraph affinity filters based on heparin coated beads	343:432	OBJECTIVES The aim of this study was to analyse the binding activity of S. aureus to Seraph affinity filters based on heparin coated beads and to identify effectors influencing this binding activity.					
36930680	1	102	theme	infections	254:263	arg1	treatment					228:236	treatment	228:236	treatment of blood stream infections with multiresistant bacteria	228:292	INTRODUCTION Extracorporeal blood purification systems represent a promising alternative for treatment of blood stream infections with multiresistant bacteria.					
36930680	4	103	theme	human	799:803	arg1	plasma					805:810	human plasma	799:810	human plasma	799:810	The binding activity of S. aureus was clearly impaired in human plasma: while a percent removal of 42% was observed in 0.9% NaCl (p-value 0.0472) using Seraph mini columns, a percent removal of only 10% was achieved in human plasma (p-value 0.0934).					
36930680	2	104	theme	Seraph	380:385	arg1	filters					396:402	Seraph affinity filters	380:402	Seraph affinity filters based on heparin coated beads	380:432	OBJECTIVES The aim of this study was to analyse the binding activity of S. aureus to Seraph affinity filters based on heparin coated beads and to identify effectors influencing this binding activity.					
36930680	1	105	theme	multiresistant	270:283	arg1	bacteria					285:292	multiresistant bacteria	270:292	multiresistant bacteria	270:292	INTRODUCTION Extracorporeal blood purification systems represent a promising alternative for treatment of blood stream infections with multiresistant bacteria.					
35671692	5	0	theme	biochemical	1085:1095	arg1	profile					1097:1103	the biochemical profile	1081:1103	the biochemical profile	1081:1103	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	6	1	from	1020 cm-1	1298:1306	arg1	peaks					1274:1278	three distinctive peaks	1256:1278	three distinctive peaks	1256:1278	Particularly, a rise in the glycogen level, marked by three distinctive peaks at 1150, 1080 and 1020 cm-1, within the living cells after the anti-diabetic drug treatments is observed.					
35671692	3	2	theme	biochemical	501:511	arg1	changes					527:533	biochemical compositional changes	501:533	biochemical compositional changes within living cells in situ	501:561	Recently, we have developed a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ and the spectral changes are shown to be highly specific to the drug applied.					
35671692	7	3	theme	FTIR	1400:1403	arg1	results					1405:1411	The live-cell FTIR results	1386:1411	The live-cell FTIR results	1386:1411	The live-cell FTIR results are confirmed by a parallel gold-standard biochemical assay, demonstrating the restoration of insulin sensitivity of the insulin-resistance cells.					
35671692	1	4	theme	enzymatic	274:282	arg1	assay					284:288	enzymatic assay	274:288	enzymatic assay	274:288	Conventional in vitro study often involves the destruction of the cells followed by purification and dilution steps before applying enzymatic assay or metabolomic analysis.					
35671692	0	5	theme	insulin-resistant	86:102	arg1	HepG2					104:108	resveratrol-treated insulin-resistant HepG2	66:108	resveratrol-treated insulin-resistant HepG2	66:108	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	0	6	from	study	11:15	arg1	HepG2					104:108	resveratrol-treated insulin-resistant HepG2	66:108	resveratrol-treated insulin-resistant HepG2	66:108	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	0	6	from	study	11:15	arg1	metformin					52:60	metformin	52:60	metformin	52:60	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	3	7	theme	compositional	513:525	arg1	changes					527:533	biochemical compositional changes	501:533	biochemical compositional changes within living cells in situ	501:561	Recently, we have developed a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ and the spectral changes are shown to be highly specific to the drug applied.					
35671692	5	8	theme	drug	1175:1178	arg1	treatment					1180:1188	the drug treatment	1171:1188	the drug treatment (p < 0.05)	1171:1199	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	8	theme	drug	1175:1178	arg1	p < 0.05					1191:1198	p < 0.05	1191:1198	p < 0.05	1191:1198	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	0	9	theme	live-cell	113:121	arg1	spectroscopy					128:139	live-cell FTIR spectroscopy	113:139	live-cell FTIR spectroscopy	113:139	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	5	10	from	sensitivity	1109:1119	arg1	cell					1031:1034	the insulin-resistant cell	1009:1034	the insulin-resistant cell	1009:1034	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	1	11	dep	in	155:156	arg1	vitro					158:162	vitro	158:162	vitro	158:162	Conventional in vitro study often involves the destruction of the cells followed by purification and dilution steps before applying enzymatic assay or metabolomic analysis.					
35671692	5	12	theme	normal	925:930	arg1	sensitivity					1109:1119	sensitivity	1109:1119	sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05)	1109:1199	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	12	theme	normal	925:930	arg1	cells					954:958	normal and insulin-resistant cells	925:958	normal and insulin-resistant cells (p < 0.05)	925:969	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	12	theme	normal	925:930	arg1	p < 0.05					961:968	p < 0.05	961:968	p < 0.05	961:968	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	12	theme	normal	925:930	arg1	lack					976:979	the lack	972:979	the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile	972:1103	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	3	13	theme	spectral	571:578	arg1	specific					611:618	specific	611:618	specific	611:618	Recently, we have developed a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ and the spectral changes are shown to be highly specific to the drug applied.					
35671692	3	13	theme	spectral	571:578	arg1	changes					580:586	the spectral changes	567:586	the spectral changes	567:586	Recently, we have developed a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ and the spectral changes are shown to be highly specific to the drug applied.					
35671692	0	14	from	metformin	52:60	arg1	study					11:15	Label-free study	0:15	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.	0:140	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	5	15	theme	insulin-resistant	936:952	arg1	sensitivity					1109:1119	sensitivity	1109:1119	sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05)	1109:1199	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	15	theme	insulin-resistant	936:952	arg1	cells					954:958	normal and insulin-resistant cells	925:958	normal and insulin-resistant cells (p < 0.05)	925:969	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	15	theme	insulin-resistant	936:952	arg1	p < 0.05					961:968	p < 0.05	961:968	p < 0.05	961:968	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	15	theme	insulin-resistant	936:952	arg1	lack					976:979	the lack	972:979	the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile	972:1103	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	1	16	theme	metabolomic	293:303	arg1	analysis					305:312	metabolomic analysis	293:312	metabolomic analysis	293:312	Conventional in vitro study often involves the destruction of the cells followed by purification and dilution steps before applying enzymatic assay or metabolomic analysis.					
35671692	6	17	from	1080	1289:1292	arg1	peaks					1274:1278	three distinctive peaks	1256:1278	three distinctive peaks	1256:1278	Particularly, a rise in the glycogen level, marked by three distinctive peaks at 1150, 1080 and 1020 cm-1, within the living cells after the anti-diabetic drug treatments is observed.					
35671692	5	18	from	cells	1159:1163	arg1	insulin					1124:1130	insulin	1124:1130	insulin from the insulin-resistant cells after the drug treatment (p < 0.05)	1124:1199	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	7	19	theme	insulin	1507:1513	arg1	sensitivity					1515:1525	insulin sensitivity	1507:1525	insulin sensitivity of the insulin-resistance cells	1507:1557	The live-cell FTIR results are confirmed by a parallel gold-standard biochemical assay, demonstrating the restoration of insulin sensitivity of the insulin-resistance cells.					
35671692	3	20	theme	living	542:547	arg1	cells					549:553	living cells	542:553	living cells	542:553	Recently, we have developed a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ and the spectral changes are shown to be highly specific to the drug applied.					
35671692	2	21	theme	time	400:403	arg1	changes					375:381	changes	375:381	changes	375:381	It is a costly and laborious process, and it cannot monitor changes as a function of time.					
35671692	2	21	theme	time	400:403	arg1	function					388:395	a function	386:395	a function of time	386:403	It is a costly and laborious process, and it cannot monitor changes as a function of time.					
35671692	0	22	theme	FTIR	123:126	arg1	spectroscopy					128:139	live-cell FTIR spectroscopy	113:139	live-cell FTIR spectroscopy	113:139	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	0	23	from	level	43:47	arg1	HepG2					104:108	resveratrol-treated insulin-resistant HepG2	66:108	resveratrol-treated insulin-resistant HepG2	66:108	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	0	23	from	level	43:47	arg1	metformin					52:60	metformin	52:60	metformin	52:60	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	6	24	theme	drug	1357:1360	arg1	treatments					1362:1371	the anti-diabetic drug treatments	1339:1371	the anti-diabetic drug treatments	1339:1371	Particularly, a rise in the glycogen level, marked by three distinctive peaks at 1150, 1080 and 1020 cm-1, within the living cells after the anti-diabetic drug treatments is observed.					
35671692	7	25	theme	sensitivity	1515:1525	arg1	restoration					1492:1502	the restoration	1488:1502	the restoration of insulin sensitivity of the insulin-resistance cells	1488:1557	The live-cell FTIR results are confirmed by a parallel gold-standard biochemical assay, demonstrating the restoration of insulin sensitivity of the insulin-resistance cells.					
35671692	3	26	dep	measure	493:499	arg1	can					480:482	can	480:482	can	480:482	Recently, we have developed a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ and the spectral changes are shown to be highly specific to the drug applied.					
35671692	3	27	theme	new	436:438	arg1	approach					466:473	a new label-free live-cell FTIR approach	434:473	a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ	434:561	Recently, we have developed a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ and the spectral changes are shown to be highly specific to the drug applied.					
35671692	6	28	from	1150	1283:1286	arg1	peaks					1274:1278	three distinctive peaks	1256:1278	three distinctive peaks	1256:1278	Particularly, a rise in the glycogen level, marked by three distinctive peaks at 1150, 1080 and 1020 cm-1, within the living cells after the anti-diabetic drug treatments is observed.					
35671692	5	29	from	cell	1031:1034	arg1	cells					954:958	normal and insulin-resistant cells	925:958	normal and insulin-resistant cells (p < 0.05)	925:969	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	29	from	cell	1031:1034	arg1	sensitivity					1109:1119	sensitivity	1109:1119	sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05)	1109:1199	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	29	from	cell	1031:1034	arg1	response/difference					984:1002	response/difference	984:1002	response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile	984:1103	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	29	from	cell	1031:1034	arg1	lack					976:979	the lack	972:979	the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile	972:1103	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	2	30	theme	laborious	334:342	arg1	It					315:316	It	315:316	It	315:316	It is a costly and laborious process, and it cannot monitor changes as a function of time.					
35671692	2	30	theme	laborious	334:342	arg1	process					344:350	a costly and laborious process	321:350	a costly and laborious process	321:350	It is a costly and laborious process, and it cannot monitor changes as a function of time.					
35671692	5	31	theme	component	839:847	arg1	analysis					849:856	principal component analysis	829:856	principal component analysis	829:856	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	0	32	theme	Label-free	0:9	arg1	study					11:15	Label-free study	0:15	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.	0:140	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	1	33	theme	cells	208:212	arg1	destruction					189:199	the destruction	185:199	the destruction of the cells followed by purification and dilution steps	185:256	Conventional in vitro study often involves the destruction of the cells followed by purification and dilution steps before applying enzymatic assay or metabolomic analysis.					
35671692	0	34	theme	intracellular	20:32	arg1	level					43:47	intracellular glycogen level	20:47	intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2	20:108	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	5	35	theme	insulin-resistant	1141:1157	arg1	cells					1159:1163	the insulin-resistant cells	1137:1163	the insulin-resistant cells	1137:1163	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	36	from	differences	877:887	arg1	profiles					908:915	the biochemical profiles	892:915	the biochemical profiles	892:915	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	2	37	theme	costly	323:328	arg1	It					315:316	It	315:316	It	315:316	It is a costly and laborious process, and it cannot monitor changes as a function of time.					
35671692	2	37	theme	costly	323:328	arg1	process					344:350	a costly and laborious process	321:350	a costly and laborious process	321:350	It is a costly and laborious process, and it cannot monitor changes as a function of time.					
35671692	3	38	theme	FTIR	461:464	arg1	approach					466:473	a new label-free live-cell FTIR approach	434:473	a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ	434:561	Recently, we have developed a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ and the spectral changes are shown to be highly specific to the drug applied.					
35671692	8	39	theme	efficacy	1612:1619	arg1	screening					1621:1629	drug efficacy screening	1607:1629	drug efficacy screening	1607:1629	Live-cell FTIR can be a complementary tool for drug efficacy screening, especially for insulin sensitizers.					
35671692	0	40	from	HepG2	104:108	arg1	study					11:15	Label-free study	0:15	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.	0:140	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	4	41	theme	anti-diabetic	713:725	arg1	metformin					734:742	metformin	734:742	metformin	734:742	In this work, we have demonstrated for the first time the effect of two anti-diabetic drugs, metformin and Resveratrol, on insulin-resistant liver cells (HepG2).					
35671692	4	41	theme	anti-diabetic	713:725	arg1	drugs					727:731	two anti-diabetic drugs	709:731	two anti-diabetic drugs	709:731	In this work, we have demonstrated for the first time the effect of two anti-diabetic drugs, metformin and Resveratrol, on insulin-resistant liver cells (HepG2).					
35671692	4	41	theme	anti-diabetic	713:725	arg1	Resveratrol					748:758	Resveratrol	748:758	Resveratrol	748:758	In this work, we have demonstrated for the first time the effect of two anti-diabetic drugs, metformin and Resveratrol, on insulin-resistant liver cells (HepG2).					
35671692	5	42	from	lack	976:979	arg1	cell					1031:1034	the insulin-resistant cell	1009:1034	the insulin-resistant cell	1009:1034	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	0	43	theme	level	43:47	arg1	study					11:15	Label-free study	0:15	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.	0:140	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	4	44	theme	first	684:688	arg1	time					690:693	the first time	680:693	the first time	680:693	In this work, we have demonstrated for the first time the effect of two anti-diabetic drugs, metformin and Resveratrol, on insulin-resistant liver cells (HepG2).					
35671692	5	45	theme	live-cell	809:817	arg1	FTIR					819:822	live-cell FTIR	809:822	live-cell FTIR	809:822	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	8	46	theme	complementary	1584:1596	arg1	tool					1598:1601	a complementary tool	1582:1601	a complementary tool for drug efficacy screening	1582:1629	Live-cell FTIR can be a complementary tool for drug efficacy screening, especially for insulin sensitizers.					
35671692	8	46	theme	complementary	1584:1596	arg1	FTIR					1570:1573	Live-cell FTIR	1560:1573	Live-cell FTIR	1560:1573	Live-cell FTIR can be a complementary tool for drug efficacy screening, especially for insulin sensitizers.					
35671692	3	47	theme	label-free	440:449	arg1	approach					466:473	a new label-free live-cell FTIR approach	434:473	a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ	434:561	Recently, we have developed a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ and the spectral changes are shown to be highly specific to the drug applied.					
35671692	0	48	theme	glycogen	34:41	arg1	level					43:47	intracellular glycogen level	20:47	intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2	20:108	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	6	49	theme	anti-diabetic	1343:1355	arg1	treatments					1362:1371	the anti-diabetic drug treatments	1339:1371	the anti-diabetic drug treatments	1339:1371	Particularly, a rise in the glycogen level, marked by three distinctive peaks at 1150, 1080 and 1020 cm-1, within the living cells after the anti-diabetic drug treatments is observed.					
35671692	7	50	theme	live-cell	1390:1398	arg1	results					1405:1411	The live-cell FTIR results	1386:1411	The live-cell FTIR results	1386:1411	The live-cell FTIR results are confirmed by a parallel gold-standard biochemical assay, demonstrating the restoration of insulin sensitivity of the insulin-resistance cells.					
35671692	3	51	theme	live-cell	451:459	arg1	approach					466:473	a new label-free live-cell FTIR approach	434:473	a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ	434:561	Recently, we have developed a new label-free live-cell FTIR approach that can directly measure biochemical compositional changes within living cells in situ and the spectral changes are shown to be highly specific to the drug applied.					
35671692	6	52	theme	distinctive	1262:1272	arg1	peaks					1274:1278	three distinctive peaks	1256:1278	three distinctive peaks	1256:1278	Particularly, a rise in the glycogen level, marked by three distinctive peaks at 1150, 1080 and 1020 cm-1, within the living cells after the anti-diabetic drug treatments is observed.					
35671692	5	53	theme	response/difference	984:1002	arg1	sensitivity					1109:1119	sensitivity	1109:1119	sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05)	1109:1199	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	53	theme	response/difference	984:1002	arg1	cells					954:958	normal and insulin-resistant cells	925:958	normal and insulin-resistant cells (p < 0.05)	925:969	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	53	theme	response/difference	984:1002	arg1	lack					976:979	the lack	972:979	the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile	972:1103	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	54	theme	profile	1097:1103	arg1	restoration					1066:1076	the restoration	1062:1076	the restoration of the biochemical profile	1062:1103	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	54	theme	profile	1097:1103	arg1	p > 0.05					1048:1055	p > 0.05	1048:1055	p > 0.05	1048:1055	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	54	theme	profile	1097:1103	arg1	insulin					1039:1045	insulin	1039:1045	insulin (p > 0.05)	1039:1056	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	8	55	theme	drug	1607:1610	arg1	screening					1621:1629	drug efficacy screening	1607:1629	drug efficacy screening	1607:1629	Live-cell FTIR can be a complementary tool for drug efficacy screening, especially for insulin sensitizers.					
35671692	7	56	theme	biochemical	1455:1465	arg1	assay					1467:1471	a parallel gold-standard biochemical assay	1430:1471	a parallel gold-standard biochemical assay	1430:1471	The live-cell FTIR results are confirmed by a parallel gold-standard biochemical assay, demonstrating the restoration of insulin sensitivity of the insulin-resistance cells.					
35671692	6	57	from	rise	1218:1221	arg1	level					1239:1243	the glycogen level	1226:1243	the glycogen level	1226:1243	Particularly, a rise in the glycogen level, marked by three distinctive peaks at 1150, 1080 and 1020 cm-1, within the living cells after the anti-diabetic drug treatments is observed.					
35671692	1	58	theme	Conventional	142:153	arg1	study					164:168	Conventional in vitro study	142:168	Conventional in vitro study	142:168	Conventional in vitro study often involves the destruction of the cells followed by purification and dilution steps before applying enzymatic assay or metabolomic analysis.					
35671692	4	59	from	effect	699:704	arg1	HepG2					795:799	HepG2	795:799	HepG2	795:799	In this work, we have demonstrated for the first time the effect of two anti-diabetic drugs, metformin and Resveratrol, on insulin-resistant liver cells (HepG2).					
35671692	4	59	from	effect	699:704	arg1	cells					788:792	insulin-resistant liver cells	764:792	insulin-resistant liver cells (HepG2)	764:800	In this work, we have demonstrated for the first time the effect of two anti-diabetic drugs, metformin and Resveratrol, on insulin-resistant liver cells (HepG2).					
35671692	5	60	theme	biochemical	896:906	arg1	profiles					908:915	the biochemical profiles	892:915	the biochemical profiles	892:915	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	5	61	theme	insulin-resistant	1013:1029	arg1	cell					1031:1034	the insulin-resistant cell	1009:1034	the insulin-resistant cell	1009:1034	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	1	62	theme	in	155:156	arg1	study					164:168	Conventional in vitro study	142:168	Conventional in vitro study	142:168	Conventional in vitro study often involves the destruction of the cells followed by purification and dilution steps before applying enzymatic assay or metabolomic analysis.					
35671692	4	63	theme	drugs	727:731	arg1	effect					699:704	the effect	695:704	the effect of two anti-diabetic drugs, metformin and Resveratrol, on insulin-resistant liver cells (HepG2)	695:800	In this work, we have demonstrated for the first time the effect of two anti-diabetic drugs, metformin and Resveratrol, on insulin-resistant liver cells (HepG2).					
35671692	1	64	theme	dilution	243:250	arg1	steps					252:256	dilution steps	243:256	dilution steps	243:256	Conventional in vitro study often involves the destruction of the cells followed by purification and dilution steps before applying enzymatic assay or metabolomic analysis.					
35671692	7	65	theme	parallel	1432:1439	arg1	assay					1467:1471	a parallel gold-standard biochemical assay	1430:1471	a parallel gold-standard biochemical assay	1430:1471	The live-cell FTIR results are confirmed by a parallel gold-standard biochemical assay, demonstrating the restoration of insulin sensitivity of the insulin-resistance cells.					
35671692	8	66	theme	Live-cell	1560:1568	arg1	tool					1598:1601	a complementary tool	1582:1601	a complementary tool for drug efficacy screening	1582:1629	Live-cell FTIR can be a complementary tool for drug efficacy screening, especially for insulin sensitizers.					
35671692	8	66	theme	Live-cell	1560:1568	arg1	FTIR					1570:1573	Live-cell FTIR	1560:1573	Live-cell FTIR	1560:1573	Live-cell FTIR can be a complementary tool for drug efficacy screening, especially for insulin sensitizers.					
35671692	0	67	theme	resveratrol-treated	66:84	arg1	HepG2					104:108	resveratrol-treated insulin-resistant HepG2	66:108	resveratrol-treated insulin-resistant HepG2	66:108	Label-free study of intracellular glycogen level in metformin and resveratrol-treated insulin-resistant HepG2 by live-cell FTIR spectroscopy.					
35671692	7	68	theme	gold-standard	1441:1453	arg1	assay					1467:1471	a parallel gold-standard biochemical assay	1430:1471	a parallel gold-standard biochemical assay	1430:1471	The live-cell FTIR results are confirmed by a parallel gold-standard biochemical assay, demonstrating the restoration of insulin sensitivity of the insulin-resistance cells.					
35671692	4	69	theme	liver	782:786	arg1	HepG2					795:799	HepG2	795:799	HepG2	795:799	In this work, we have demonstrated for the first time the effect of two anti-diabetic drugs, metformin and Resveratrol, on insulin-resistant liver cells (HepG2).					
35671692	4	69	theme	liver	782:786	arg1	cells					788:792	insulin-resistant liver cells	764:792	insulin-resistant liver cells (HepG2)	764:800	In this work, we have demonstrated for the first time the effect of two anti-diabetic drugs, metformin and Resveratrol, on insulin-resistant liver cells (HepG2).					
35671692	5	70	theme	principal	829:837	arg1	analysis					849:856	principal component analysis	829:856	principal component analysis	829:856	Using live-cell FTIR with principal component analysis, we have shown the differences in the biochemical profiles between normal and insulin-resistant cells (p < 0.05), the lack of response/difference from the insulin-resistant cell to insulin (p > 0.05) and the restoration of the biochemical profile and sensitivity to insulin from the insulin-resistant cells after the drug treatment (p < 0.05).					
35671692	8	71	theme	insulin	1647:1653	arg1	sensitizers					1655:1665	insulin sensitizers	1647:1665	insulin sensitizers	1647:1665	Live-cell FTIR can be a complementary tool for drug efficacy screening, especially for insulin sensitizers.					
35671692	6	72	theme	living	1320:1325	arg1	cells					1327:1331	the living cells	1316:1331	the living cells	1316:1331	Particularly, a rise in the glycogen level, marked by three distinctive peaks at 1150, 1080 and 1020 cm-1, within the living cells after the anti-diabetic drug treatments is observed.					
35671692	4	73	theme	insulin-resistant	764:780	arg1	HepG2					795:799	HepG2	795:799	HepG2	795:799	In this work, we have demonstrated for the first time the effect of two anti-diabetic drugs, metformin and Resveratrol, on insulin-resistant liver cells (HepG2).					
35671692	4	73	theme	insulin-resistant	764:780	arg1	cells					788:792	insulin-resistant liver cells	764:792	insulin-resistant liver cells (HepG2)	764:800	In this work, we have demonstrated for the first time the effect of two anti-diabetic drugs, metformin and Resveratrol, on insulin-resistant liver cells (HepG2).					
35671692	7	74	theme	cells	1553:1557	arg1	sensitivity					1515:1525	insulin sensitivity	1507:1525	insulin sensitivity of the insulin-resistance cells	1507:1557	The live-cell FTIR results are confirmed by a parallel gold-standard biochemical assay, demonstrating the restoration of insulin sensitivity of the insulin-resistance cells.					
35671692	6	75	theme	glycogen	1230:1237	arg1	level					1239:1243	the glycogen level	1226:1243	the glycogen level	1226:1243	Particularly, a rise in the glycogen level, marked by three distinctive peaks at 1150, 1080 and 1020 cm-1, within the living cells after the anti-diabetic drug treatments is observed.					
35671692	7	76	theme	insulin-resistance	1534:1551	arg1	cells					1553:1557	the insulin-resistance cells	1530:1557	the insulin-resistance cells	1530:1557	The live-cell FTIR results are confirmed by a parallel gold-standard biochemical assay, demonstrating the restoration of insulin sensitivity of the insulin-resistance cells.					
35367270	7	0	theme	F-value	1143:1149	arg1	value					1117:1121	determination (R2) value	1098:1121	determination (R2) value of 0.9997 and model F-value of 1099.97	1098:1160	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	10	1	theme	effective	1460:1468	arg1	performance					1481:1491	The effective adsorption performance	1456:1491	The effective adsorption performance	1456:1491	Adsorption of As in the presence of other competitive ions was not significantly affected The effective adsorption performance also sustained even after ten adsorption-desorption cycles, indicating favorable reusability.					
35367270	2	2	theme	chitosan/graphene	314:330	arg1	oxide					332:336	Zn-BDC@chitosan/graphene oxide	307:336	Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO)	307:351	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	2	2	theme	chitosan/graphene	314:330	arg1	CT/GO					346:350	Zn-BDC@CT/GO	339:350	Zn-BDC@CT/GO	339:350	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	5	3	dep	five-level	740:749	arg1	four-factor					752:762	four-factor	752:762	four-factor	752:762	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	7	4	theme	experimental	1023:1034	arg1	data					1036:1039	the experimental data	1019:1039	the experimental data	1019:1039	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	2	5	theme	chitosan/carbon	262:276	arg1	nanotube					278:285	The Zn-BDC@chitosan/carbon nanotube	251:285	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT)	251:301	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	2	5	theme	chitosan/carbon	262:276	arg1	CT/CNT					295:300	Zn-BDC@CT/CNT	288:300	Zn-BDC@CT/CNT	288:300	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	2	6	theme	Zn-BDC	255:260	arg1	nanotube					278:285	The Zn-BDC@chitosan/carbon nanotube	251:285	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT)	251:301	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	2	6	theme	Zn-BDC	255:260	arg1	CT/CNT					295:300	Zn-BDC@CT/CNT	288:300	Zn-BDC@CT/CNT	288:300	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	7	7	theme	good	1007:1010	arg1	fit					1012:1014	good fit	1007:1014	good fit of the experimental data to the second-order polynomial model	1007:1076	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	2	8	theme	@	294:294	arg1	nanotube					278:285	The Zn-BDC@chitosan/carbon nanotube	251:285	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT)	251:301	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	2	8	theme	@	294:294	arg1	CT/CNT					295:300	Zn-BDC@CT/CNT	288:300	Zn-BDC@CT/CNT	288:300	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	10	9	theme	adsorption-desorption	1523:1543	arg1	cycles					1545:1550	ten adsorption-desorption cycles	1519:1550	ten adsorption-desorption cycles	1519:1550	Adsorption of As in the presence of other competitive ions was not significantly affected The effective adsorption performance also sustained even after ten adsorption-desorption cycles, indicating favorable reusability.					
35367270	3	10	theme	higher	534:539	arg1	capacity					552:559	a higher adsorption capacity	532:559	a higher adsorption capacity	532:559	Results of adsorption experiments showed that the Zn-BDC@CT/GO possessed a higher adsorption capacity than that of the Zn-BDC@CT/CNT.					
35367270	4	11	from	study	595:599	arg1	adsorption					608:617	the adsorption	604:617	the adsorption of As onto Zn-BDC@CT/GO	604:641	A study on the adsorption of As onto Zn-BDC@CT/GO was conducted and the process parameters were optimized by response surface methodology (RSM).					
35367270	8	12	theme	pseudo-second-order	1195:1213	arg1	model					1215:1219	the pseudo-second-order model	1191:1219	the pseudo-second-order model	1191:1219	The adsorption matched with the pseudo-second-order model and the Freundlich model.					
35367270	10	13	theme	favorable	1564:1572	arg1	reusability					1574:1584	favorable reusability	1564:1584	favorable reusability	1564:1584	Adsorption of As in the presence of other competitive ions was not significantly affected The effective adsorption performance also sustained even after ten adsorption-desorption cycles, indicating favorable reusability.					
35367270	5	14	theme	various	836:842	arg1	parameters					852:861	various process parameters	836:861	various process parameters	836:861	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	10	15	from	presence	1390:1397	arg1	Adsorption					1366:1375	Adsorption	1366:1375	Adsorption of As in the presence of other competitive ions	1366:1423	Adsorption of As in the presence of other competitive ions was not significantly affected The effective adsorption performance also sustained even after ten adsorption-desorption cycles, indicating favorable reusability.					
35367270	4	16	theme	process	665:671	arg1	parameters					673:682	the process parameters	661:682	the process parameters	661:682	A study on the adsorption of As onto Zn-BDC@CT/GO was conducted and the process parameters were optimized by response surface methodology (RSM).					
35367270	5	17	theme	parameters	852:861	arg1	effect					826:831	the effect	822:831	the effect of various process parameters on As uptake from aqueous solution	822:896	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	4	18	theme	@	636:636	arg1	CT/GO					637:641	Zn-BDC@CT/GO	630:641	Zn-BDC@CT/GO	630:641	A study on the adsorption of As onto Zn-BDC@CT/GO was conducted and the process parameters were optimized by response surface methodology (RSM).					
35367270	7	19	theme	model	1137:1141	arg1	F-value					1143:1149	model F-value	1137:1149	model F-value	1137:1149	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	3	20	theme	@	584:584	arg1	CT/CNT					585:590	the Zn-BDC@CT/CNT	574:590	the Zn-BDC@CT/CNT	574:590	Results of adsorption experiments showed that the Zn-BDC@CT/GO possessed a higher adsorption capacity than that of the Zn-BDC@CT/CNT.					
35367270	10	21	theme	As	1380:1381	arg1	Adsorption					1366:1375	Adsorption	1366:1375	Adsorption of As in the presence of other competitive ions	1366:1423	Adsorption of As in the presence of other competitive ions was not significantly affected The effective adsorption performance also sustained even after ten adsorption-desorption cycles, indicating favorable reusability.					
35367270	10	22	dep	affected	1447:1454	arg1	sustained					1498:1506	sustained	1498:1506	sustained	1498:1506	Adsorption of As in the presence of other competitive ions was not significantly affected The effective adsorption performance also sustained even after ten adsorption-desorption cycles, indicating favorable reusability.					
35367270	1	23	theme	arsenic	154:160	arg1	removal					139:145	removal	139:145	removal of the arsenic (As)	139:165	In this work removal of the arsenic (As) spiked in water through adsorption using synthesized nanocomposites as a adsorbent.					
35367270	4	24	theme	surface	711:717	arg1	RSM					732:734	RSM	732:734	RSM	732:734	A study on the adsorption of As onto Zn-BDC@CT/GO was conducted and the process parameters were optimized by response surface methodology (RSM).					
35367270	4	24	theme	surface	711:717	arg1	methodology					719:729	response surface methodology	702:729	response surface methodology (RSM)	702:735	A study on the adsorption of As onto Zn-BDC@CT/GO was conducted and the process parameters were optimized by response surface methodology (RSM).					
35367270	7	25	theme	value	1117:1121	arg1	coefficient					1083:1093	coefficient	1083:1093	coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97	1083:1160	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	0	26	theme	adsorption	106:115	arg1	studies					117:123	adsorption studies	106:123	adsorption studies	106:123	Removal of arsenic by metal organic framework/chitosan/carbon nanocomposites: Modeling, optimization, and adsorption studies.					
35367270	0	27	theme	organic	28:34	arg1	nanocomposites					62:75	metal organic framework/chitosan/carbon nanocomposites	22:75	metal organic framework/chitosan/carbon nanocomposites	22:75	Removal of arsenic by metal organic framework/chitosan/carbon nanocomposites: Modeling, optimization, and adsorption studies.					
35367270	2	28	theme	nanotube/graphene	407:423	arg1	oxide					425:429	carbon nanotube/graphene oxide	400:429	carbon nanotube/graphene oxide	400:429	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	7	29	theme	determination	1098:1110	arg1	value					1117:1121	determination (R2) value	1098:1121	determination (R2) value of 0.9997 and model F-value of 1099.97	1098:1160	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	5	30	theme	As	866:867	arg1	uptake					869:874	As uptake	866:874	As uptake from aqueous solution	866:896	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	2	31	theme	organic	381:387	arg1	framework					389:397	metal organic framework	375:397	metal organic framework	375:397	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	5	32	theme	central	764:770	arg1	CCD					790:792	CCD	790:792	CCD	790:792	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	5	32	theme	central	764:770	arg1	design					782:787	A five-level, four-factor central composite design	738:787	A five-level, four-factor central composite design (CCD)	738:793	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	3	33	theme	Zn-BDC	509:514	arg1	CT/GO					516:520	the Zn-BDC@CT/GO	505:520	the Zn-BDC@CT/GO	505:520	Results of adsorption experiments showed that the Zn-BDC@CT/GO possessed a higher adsorption capacity than that of the Zn-BDC@CT/CNT.					
35367270	8	34	theme	Freundlich	1229:1238	arg1	model					1240:1244	the Freundlich model	1225:1244	the Freundlich model	1225:1244	The adsorption matched with the pseudo-second-order model and the Freundlich model.					
35367270	10	35	theme	competitive	1408:1418	arg1	ions					1420:1423	other competitive ions	1402:1423	other competitive ions	1402:1423	Adsorption of As in the presence of other competitive ions was not significantly affected The effective adsorption performance also sustained even after ten adsorption-desorption cycles, indicating favorable reusability.					
35367270	0	36	theme	metal	22:26	arg1	nanocomposites					62:75	metal organic framework/chitosan/carbon nanocomposites	22:75	metal organic framework/chitosan/carbon nanocomposites	22:75	Removal of arsenic by metal organic framework/chitosan/carbon nanocomposites: Modeling, optimization, and adsorption studies.					
35367270	6	37	theme	experimental	945:956	arg1	data					958:961	20 adsorption experimental data	931:961	20 adsorption experimental data	931:961	By using this design a total of 20 adsorption experimental data were fitted.					
35367270	2	38	theme	@	313:313	arg1	oxide					332:336	Zn-BDC@chitosan/graphene oxide	307:336	Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO)	307:351	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	2	38	theme	@	313:313	arg1	CT/GO					346:350	Zn-BDC@CT/GO	339:350	Zn-BDC@CT/GO	339:350	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	0	39	theme	framework/chitosan/carbon	36:60	arg1	nanocomposites					62:75	metal organic framework/chitosan/carbon nanocomposites	22:75	metal organic framework/chitosan/carbon nanocomposites	22:75	Removal of arsenic by metal organic framework/chitosan/carbon nanocomposites: Modeling, optimization, and adsorption studies.					
35367270	9	40	theme	adsorption	1304:1313	arg1	nature					1294:1299	the nature	1290:1299	the nature of adsorption	1290:1313	The thermodynamic parameters revealed that the nature of adsorption was feasible, spontaneous and endothermic process.					
35367270	9	40	theme	adsorption	1304:1313	arg1	process					1357:1363	feasible, spontaneous and endothermic process	1319:1363	feasible, spontaneous and endothermic process	1319:1363	The thermodynamic parameters revealed that the nature of adsorption was feasible, spontaneous and endothermic process.					
35367270	3	41	theme	@	515:515	arg1	CT/GO					516:520	the Zn-BDC@CT/GO	505:520	the Zn-BDC@CT/GO	505:520	Results of adsorption experiments showed that the Zn-BDC@CT/GO possessed a higher adsorption capacity than that of the Zn-BDC@CT/CNT.					
35367270	2	42	theme	Zn-BDC	339:344	arg1	oxide					332:336	Zn-BDC@chitosan/graphene oxide	307:336	Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO)	307:351	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	2	42	theme	Zn-BDC	339:344	arg1	CT/GO					346:350	Zn-BDC@CT/GO	339:350	Zn-BDC@CT/GO	339:350	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	9	43	theme	feasible	1319:1326	arg1	nature					1294:1299	the nature	1290:1299	the nature of adsorption	1290:1313	The thermodynamic parameters revealed that the nature of adsorption was feasible, spontaneous and endothermic process.					
35367270	9	43	theme	feasible	1319:1326	arg1	process					1357:1363	feasible, spontaneous and endothermic process	1319:1363	feasible, spontaneous and endothermic process	1319:1363	The thermodynamic parameters revealed that the nature of adsorption was feasible, spontaneous and endothermic process.					
35367270	10	44	theme	adsorption	1470:1479	arg1	performance					1481:1491	The effective adsorption performance	1456:1491	The effective adsorption performance	1456:1491	Adsorption of As in the presence of other competitive ions was not significantly affected The effective adsorption performance also sustained even after ten adsorption-desorption cycles, indicating favorable reusability.					
35367270	7	45	theme	data	1036:1039	arg1	fit					1012:1014	good fit	1007:1014	good fit of the experimental data to the second-order polynomial model	1007:1076	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	3	46	theme	experiments	481:491	arg1	Results					459:465	Results	459:465	Results of adsorption experiments	459:491	Results of adsorption experiments showed that the Zn-BDC@CT/GO possessed a higher adsorption capacity than that of the Zn-BDC@CT/CNT.					
35367270	9	47	theme	spontaneous	1329:1339	arg1	nature					1294:1299	the nature	1290:1299	the nature of adsorption	1290:1313	The thermodynamic parameters revealed that the nature of adsorption was feasible, spontaneous and endothermic process.					
35367270	9	47	theme	spontaneous	1329:1339	arg1	process					1357:1363	feasible, spontaneous and endothermic process	1319:1363	feasible, spontaneous and endothermic process	1319:1363	The thermodynamic parameters revealed that the nature of adsorption was feasible, spontaneous and endothermic process.					
35367270	5	48	from	solution	889:896	arg1	uptake					869:874	As uptake	866:874	As uptake from aqueous solution	866:896	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	9	49	theme	endothermic	1345:1355	arg1	nature					1294:1299	the nature	1290:1299	the nature of adsorption	1290:1313	The thermodynamic parameters revealed that the nature of adsorption was feasible, spontaneous and endothermic process.					
35367270	9	49	theme	endothermic	1345:1355	arg1	process					1357:1363	feasible, spontaneous and endothermic process	1319:1363	feasible, spontaneous and endothermic process	1319:1363	The thermodynamic parameters revealed that the nature of adsorption was feasible, spontaneous and endothermic process.					
35367270	3	50	theme	adsorption	541:550	arg1	capacity					552:559	a higher adsorption capacity	532:559	a higher adsorption capacity	532:559	Results of adsorption experiments showed that the Zn-BDC@CT/GO possessed a higher adsorption capacity than that of the Zn-BDC@CT/CNT.					
35367270	2	51	theme	@	261:261	arg1	nanotube					278:285	The Zn-BDC@chitosan/carbon nanotube	251:285	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT)	251:301	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	2	51	theme	@	261:261	arg1	CT/CNT					295:300	Zn-BDC@CT/CNT	288:300	Zn-BDC@CT/CNT	288:300	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	7	52	theme	second-order	1048:1059	arg1	model					1072:1076	the second-order polynomial model	1044:1076	the second-order polynomial model	1044:1076	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	7	53	theme	0.9997	1126:1131	arg1	value					1117:1121	determination (R2) value	1098:1121	determination (R2) value of 0.9997 and model F-value of 1099.97	1098:1160	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	5	54	used	used	804:807	arg2	CCD					790:792	CCD	790:792	CCD	790:792	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	5	54	used	used	804:807	arg2	design					782:787	A five-level, four-factor central composite design	738:787	A five-level, four-factor central composite design (CCD)	738:793	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	10	55	from	Adsorption	1366:1375	arg1	presence					1390:1397	the presence	1386:1397	the presence of other competitive ions	1386:1423	Adsorption of As in the presence of other competitive ions was not significantly affected The effective adsorption performance also sustained even after ten adsorption-desorption cycles, indicating favorable reusability.					
35367270	4	56	theme	As	622:623	arg1	adsorption					608:617	the adsorption	604:617	the adsorption of As onto Zn-BDC@CT/GO	604:641	A study on the adsorption of As onto Zn-BDC@CT/GO was conducted and the process parameters were optimized by response surface methodology (RSM).					
35367270	7	57	theme	regression	980:989	arg1	analysis					991:998	The regression analysis	976:998	The regression analysis	976:998	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	2	58	theme	Zn-BDC	288:293	arg1	nanotube					278:285	The Zn-BDC@chitosan/carbon nanotube	251:285	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT)	251:301	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	2	58	theme	Zn-BDC	288:293	arg1	CT/CNT					295:300	Zn-BDC@CT/CNT	288:300	Zn-BDC@CT/CNT	288:300	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	5	59	theme	composite	772:780	arg1	CCD					790:792	CCD	790:792	CCD	790:792	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	5	59	theme	composite	772:780	arg1	design					782:787	A five-level, four-factor central composite design	738:787	A five-level, four-factor central composite design (CCD)	738:793	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	2	60	theme	Zn-BDC	307:312	arg1	oxide					332:336	Zn-BDC@chitosan/graphene oxide	307:336	Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO)	307:351	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	2	60	theme	Zn-BDC	307:312	arg1	CT/GO					346:350	Zn-BDC@CT/GO	339:350	Zn-BDC@CT/GO	339:350	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	5	61	theme	process	844:850	arg1	parameters					852:861	various process parameters	836:861	various process parameters	836:861	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	6	62	theme	adsorption	934:943	arg1	data					958:961	20 adsorption experimental data	931:961	20 adsorption experimental data	931:961	By using this design a total of 20 adsorption experimental data were fitted.					
35367270	3	63	theme	Zn-BDC	578:583	arg1	CT/CNT					585:590	the Zn-BDC@CT/CNT	574:590	the Zn-BDC@CT/CNT	574:590	Results of adsorption experiments showed that the Zn-BDC@CT/GO possessed a higher adsorption capacity than that of the Zn-BDC@CT/CNT.					
35367270	4	64	theme	Zn-BDC	630:635	arg1	CT/GO					637:641	Zn-BDC@CT/GO	630:641	Zn-BDC@CT/GO	630:641	A study on the adsorption of As onto Zn-BDC@CT/GO was conducted and the process parameters were optimized by response surface methodology (RSM).					
35367270	0	65	theme	arsenic	11:17	arg1	Removal					0:6	Removal	0:6	Removal of arsenic by metal organic framework/chitosan/carbon nanocomposites	0:75	Removal of arsenic by metal organic framework/chitosan/carbon nanocomposites: Modeling, optimization, and adsorption studies.					
35367270	5	66	from	effect	826:831	arg1	uptake					869:874	As uptake	866:874	As uptake from aqueous solution	866:896	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	5	67	theme	aqueous	881:887	arg1	solution					889:896	aqueous solution	881:896	aqueous solution	881:896	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	4	68	theme	response	702:709	arg1	RSM					732:734	RSM	732:734	RSM	732:734	A study on the adsorption of As onto Zn-BDC@CT/GO was conducted and the process parameters were optimized by response surface methodology (RSM).					
35367270	4	68	theme	response	702:709	arg1	methodology					719:729	response surface methodology	702:729	response surface methodology (RSM)	702:735	A study on the adsorption of As onto Zn-BDC@CT/GO was conducted and the process parameters were optimized by response surface methodology (RSM).					
35367270	5	69	theme	five-level	740:749	arg1	CCD					790:792	CCD	790:792	CCD	790:792	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	5	69	theme	five-level	740:749	arg1	design					782:787	A five-level, four-factor central composite design	738:787	A five-level, four-factor central composite design (CCD)	738:793	A five-level, four-factor central composite design (CCD) has been used to determine the effect of various process parameters on As uptake from aqueous solution.					
35367270	2	70	theme	carbon	400:405	arg1	oxide					425:429	carbon nanotube/graphene oxide	400:429	carbon nanotube/graphene oxide	400:429	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	1	71	dep	arsenic	154:160	arg1	As					163:164	As	163:164	As	163:164	In this work removal of the arsenic (As) spiked in water through adsorption using synthesized nanocomposites as a adsorbent.					
35367270	2	72	theme	@	345:345	arg1	oxide					332:336	Zn-BDC@chitosan/graphene oxide	307:336	Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO)	307:351	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	2	72	theme	@	345:345	arg1	CT/GO					346:350	Zn-BDC@CT/GO	339:350	Zn-BDC@CT/GO	339:350	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	9	73	theme	thermodynamic	1251:1263	arg1	parameters					1265:1274	The thermodynamic parameters	1247:1274	The thermodynamic parameters	1247:1274	The thermodynamic parameters revealed that the nature of adsorption was feasible, spontaneous and endothermic process.					
35367270	0	74	dep	Modeling	78:85	arg1	Removal					0:6	Removal	0:6	Removal of arsenic by metal organic framework/chitosan/carbon nanocomposites	0:75	Removal of arsenic by metal organic framework/chitosan/carbon nanocomposites: Modeling, optimization, and adsorption studies.					
35367270	10	75	theme	ions	1420:1423	arg1	presence					1390:1397	the presence	1386:1397	the presence of other competitive ions	1386:1423	Adsorption of As in the presence of other competitive ions was not significantly affected The effective adsorption performance also sustained even after ten adsorption-desorption cycles, indicating favorable reusability.					
35367270	2	76	theme	metal	375:379	arg1	framework					389:397	metal organic framework	375:397	metal organic framework	375:397	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	7	77	theme	polynomial	1061:1070	arg1	model					1072:1076	the second-order polynomial model	1044:1076	the second-order polynomial model	1044:1076	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	6	78	theme	data	958:961	arg1	total					922:926	a total	920:926	a total of 20 adsorption experimental data	920:961	By using this design a total of 20 adsorption experimental data were fitted.					
35367270	2	79	theme	natural	435:441	arg1	polysaccharide					443:456	natural polysaccharide	435:456	natural polysaccharide	435:456	The Zn-BDC@chitosan/carbon nanotube (Zn-BDC@CT/CNT) and Zn-BDC@chitosan/graphene oxide (Zn-BDC@CT/GO) were synthesized from metal organic framework, carbon nanotube/graphene oxide and natural polysaccharide.					
35367270	10	80	theme	other	1402:1406	arg1	ions					1420:1423	other competitive ions	1402:1423	other competitive ions	1402:1423	Adsorption of As in the presence of other competitive ions was not significantly affected The effective adsorption performance also sustained even after ten adsorption-desorption cycles, indicating favorable reusability.					
35367270	7	81	theme	R2	1113:1114	arg1	value					1117:1121	determination (R2) value	1098:1121	determination (R2) value of 0.9997 and model F-value of 1099.97	1098:1160	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	3	82	contain	possessed	522:530	arg1	CT/GO					516:520	the Zn-BDC@CT/GO	505:520	the Zn-BDC@CT/GO	505:520	Results of adsorption experiments showed that the Zn-BDC@CT/GO possessed a higher adsorption capacity than that of the Zn-BDC@CT/CNT.					
35367270	3	82	contain	possessed	522:530	arg2	capacity					552:559	a higher adsorption capacity	532:559	a higher adsorption capacity	532:559	Results of adsorption experiments showed that the Zn-BDC@CT/GO possessed a higher adsorption capacity than that of the Zn-BDC@CT/CNT.					
35367270	3	83	theme	adsorption	470:479	arg1	experiments					481:491	adsorption experiments	470:491	adsorption experiments	470:491	Results of adsorption experiments showed that the Zn-BDC@CT/GO possessed a higher adsorption capacity than that of the Zn-BDC@CT/CNT.					
35367270	1	84	theme	synthesized	208:218	arg1	nanocomposites					220:233	synthesized nanocomposites	208:233	synthesized nanocomposites	208:233	In this work removal of the arsenic (As) spiked in water through adsorption using synthesized nanocomposites as a adsorbent.					
35367270	1	84	theme	synthesized	208:218	arg1	adsorbent					240:248	adsorbent	240:248	adsorbent	240:248	In this work removal of the arsenic (As) spiked in water through adsorption using synthesized nanocomposites as a adsorbent.					
35367270	7	85	theme	1099.97	1154:1160	arg1	0.9997					1126:1131	0.9997	1126:1131	0.9997	1126:1131	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
35367270	7	85	theme	1099.97	1154:1160	arg1	F-value					1143:1149	model F-value	1137:1149	model F-value	1137:1149	The regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2) value of 0.9997 and model F-value of 1099.97.					
37330103	0	0	theme	gluten	85:90	arg1	behavior					73:80	the dynamic transition behavior	50:80	the dynamic transition behavior of gluten	50:90	Wheat starch particle size distribution regulates the dynamic transition behavior of gluten at different stages of dough mixing.					
37330103	9	1	theme	correlation	1324:1334	arg1	analysis					1336:1343	The correlation analysis	1320:1343	The correlation analysis	1320:1343	The correlation analysis indicated that starch particle size distribution could influence noodle quality by changing the gluten network.					
37330103	7	2	theme	highest	1161:1167	arg1	stability					1175:1183	the highest dough stability time and the lowest degree of softening	1157:1223	stability	1175:1183	Farinographic properties indicated that 25A-75B composite flour had the highest dough stability time and the lowest degree of softening.					
37330103	7	3	contain	had	1153:1155	arg2	stability					1175:1183	the highest dough stability time and the lowest degree of softening	1157:1223	stability	1175:1183	Farinographic properties indicated that 25A-75B composite flour had the highest dough stability time and the lowest degree of softening.					
37330103	7	3	contain	had	1153:1155	arg2	degree					1205:1210	the lowest degree	1194:1210	the lowest degree of softening	1194:1223	Farinographic properties indicated that 25A-75B composite flour had the highest dough stability time and the lowest degree of softening.					
37330103	7	3	contain	had	1153:1155	arg1	flour					1147:1151	25A-75B composite flour	1129:1151	25A-75B composite flour	1129:1151	Farinographic properties indicated that 25A-75B composite flour had the highest dough stability time and the lowest degree of softening.					
37330103	10	4	theme	dough	1514:1518	arg1	characteristics					1520:1534	dough characteristics	1514:1534	dough characteristics	1514:1534	The paper can provide theoretical support for regulating dough characteristics by adjusting the starch granule size distribution.					
37330103	10	5	theme	size	1568:1571	arg1	distribution					1573:1584	the starch granule size distribution	1549:1584	the starch granule size distribution	1549:1584	The paper can provide theoretical support for regulating dough characteristics by adjusting the starch granule size distribution.					
37330103	7	6	theme	dough	1169:1173	arg1	stability					1175:1183	the highest dough stability time and the lowest degree of softening	1157:1223	stability	1175:1183	Farinographic properties indicated that 25A-75B composite flour had the highest dough stability time and the lowest degree of softening.					
37330103	1	7	theme	morphology	157:166	arg1	distribution					168:179	the morphology distribution	153:179	the morphology distribution	153:179	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
37330103	4	8	from	content	743:749	arg1	system					764:769	the dough system	754:769	the dough system	754:769	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	1	9	theme	dough	265:269	arg1	stage					278:282	dough mixing stage	265:282	dough mixing stage	265:282	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
37330103	0	10	theme	different	95:103	arg1	stages					105:110	different stages	95:110	different stages of dough mixing	95:126	Wheat starch particle size distribution regulates the dynamic transition behavior of gluten at different stages of dough mixing.					
37330103	7	11	theme	composite	1137:1145	arg1	flour					1147:1151	25A-75B composite flour	1129:1151	25A-75B composite flour	1129:1151	Farinographic properties indicated that 25A-75B composite flour had the highest dough stability time and the lowest degree of softening.					
37330103	4	12	theme	dense	813:817	arg1	network					839:845	a more continuous, dense, and ordered gluten network	794:845	a more continuous, dense, and ordered gluten network	794:845	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	1	13	theme	mixing	271:276	arg1	stage					278:282	dough mixing stage	265:282	dough mixing stage	265:282	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
37330103	3	14	theme	wheat	594:598	arg1	starch					600:605	wheat starch	594:605	wheat starch with different particle sizes and gluten protein	594:654	Appropriate mixing (9 min) enhanced the interaction between wheat starch with different particle sizes and gluten protein.					
37330103	5	15	theme	granules	964:971	arg1	arrangement					937:947	the arrangement	933:947	the arrangement of A-/B-starch granules and gluten	933:982	The 50A-50B and 25A-75B doughs mixed for 9 min exhibited a dense gluten network, and the arrangement of A-/B-starch granules and gluten was tight and ordered.					
37330103	9	16	theme	starch	1360:1365	arg1	distribution					1381:1392	starch particle size distribution	1360:1392	starch particle size distribution	1360:1392	The correlation analysis indicated that starch particle size distribution could influence noodle quality by changing the gluten network.					
37330103	5	17	theme	gluten	977:982	arg1	arrangement					937:947	the arrangement	933:947	the arrangement of A-/B-starch granules and gluten	933:982	The 50A-50B and 25A-75B doughs mixed for 9 min exhibited a dense gluten network, and the arrangement of A-/B-starch granules and gluten was tight and ordered.					
37330103	6	18	theme	coil	1073:1076	arg1	structure					1078:1086	random coil structure	1066:1086	random coil structure	1066:1086	The addition of B-starch increased α-helixes, β-turns, and random coil structure.					
37330103	4	19	from	increase	722:729	arg1	content					743:749	B-starch content	734:749	B-starch content in the dough system	734:769	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	2	20	theme	glutenin	420:427	arg1	depolymerization					442:457	glutenin macropolymer depolymerization	420:457	glutenin macropolymer depolymerization	420:457	Research results indicated that mixing process induced glutenin macropolymer depolymerization, and promoted the monomeric protein conversion into the polymeric protein.					
37330103	9	21	theme	particle	1367:1374	arg1	distribution					1381:1392	starch particle size distribution	1360:1392	starch particle size distribution	1360:1392	The correlation analysis indicated that starch particle size distribution could influence noodle quality by changing the gluten network.					
37330103	8	22	theme	maximum	1255:1261	arg1	hardness					1263:1270	maximum hardness	1255:1270	maximum hardness	1255:1270	The 25A-75B noodle displayed maximum hardness, cohesiveness, chewiness, and tensile strength.					
37330103	10	23	theme	starch	1553:1558	arg1	distribution					1573:1584	the starch granule size distribution	1549:1584	the starch granule size distribution	1549:1584	The paper can provide theoretical support for regulating dough characteristics by adjusting the starch granule size distribution.					
37330103	2	24	theme	monomeric	477:485	arg1	conversion					495:504	the monomeric protein conversion	473:504	the monomeric protein conversion into the polymeric protein	473:531	Research results indicated that mixing process induced glutenin macropolymer depolymerization, and promoted the monomeric protein conversion into the polymeric protein.					
37330103	9	25	theme	gluten	1441:1446	arg1	network					1448:1454	the gluten network	1437:1454	the gluten network	1437:1454	The correlation analysis indicated that starch particle size distribution could influence noodle quality by changing the gluten network.					
37330103	0	26	theme	starch	6:11	arg1	distribution					27:38	Wheat starch particle size distribution	0:38	Wheat starch particle size distribution	0:38	Wheat starch particle size distribution regulates the dynamic transition behavior of gluten at different stages of dough mixing.					
37330103	9	27	theme	size	1376:1379	arg1	distribution					1381:1392	starch particle size distribution	1360:1392	starch particle size distribution	1360:1392	The correlation analysis indicated that starch particle size distribution could influence noodle quality by changing the gluten network.					
37330103	1	28	with	interaction	304:314	arg1	protein					356:362	protein	356:362	protein	356:362	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
37330103	1	28	with	interaction	304:314	arg1	sizes					346:350	different sizes	336:350	different sizes	336:350	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
37330103	3	29	theme	different	612:620	arg1	sizes					631:635	different particle sizes	612:635	different particle sizes	612:635	Appropriate mixing (9 min) enhanced the interaction between wheat starch with different particle sizes and gluten protein.					
37330103	3	30	with	starch	600:605	arg1	sizes					631:635	different particle sizes	612:635	different particle sizes	612:635	Appropriate mixing (9 min) enhanced the interaction between wheat starch with different particle sizes and gluten protein.					
37330103	3	30	with	starch	600:605	arg1	protein					648:654	gluten protein	641:654	gluten protein	641:654	Appropriate mixing (9 min) enhanced the interaction between wheat starch with different particle sizes and gluten protein.					
37330103	1	31	theme	molecular	182:190	arg1	structure					192:200	molecular structure	182:200	molecular structure	182:200	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
37330103	0	32	theme	Wheat	0:4	arg1	distribution					27:38	Wheat starch particle size distribution	0:38	Wheat starch particle size distribution	0:38	Wheat starch particle size distribution regulates the dynamic transition behavior of gluten at different stages of dough mixing.					
37330103	7	33	theme	25A-75B	1129:1135	arg1	flour					1147:1151	25A-75B composite flour	1129:1151	25A-75B composite flour	1129:1151	Farinographic properties indicated that 25A-75B composite flour had the highest dough stability time and the lowest degree of softening.					
37330103	3	34	theme	particle	622:629	arg1	sizes					631:635	different particle sizes	612:635	different particle sizes	612:635	Appropriate mixing (9 min) enhanced the interaction between wheat starch with different particle sizes and gluten protein.					
37330103	2	35	theme	mixing	397:402	arg1	process					404:410	mixing process	397:410	mixing process	397:410	Research results indicated that mixing process induced glutenin macropolymer depolymerization, and promoted the monomeric protein conversion into the polymeric protein.					
37330103	0	36	theme	size	22:25	arg1	distribution					27:38	Wheat starch particle size distribution	0:38	Wheat starch particle size distribution	0:38	Wheat starch particle size distribution regulates the dynamic transition behavior of gluten at different stages of dough mixing.					
37330103	5	37	theme	50A-50B	852:858	arg1	doughs					872:877	The 50A-50B and 25A-75B doughs	848:877	The 50A-50B and 25A-75B doughs mixed for 9 min	848:893	The 50A-50B and 25A-75B doughs mixed for 9 min exhibited a dense gluten network, and the arrangement of A-/B-starch granules and gluten was tight and ordered.					
37330103	0	38	theme	mixing	121:126	arg1	stages					105:110	different stages	95:110	different stages of dough mixing	95:126	Wheat starch particle size distribution regulates the dynamic transition behavior of gluten at different stages of dough mixing.					
37330103	4	39	theme	continuous	801:810	arg1	network					839:845	a more continuous, dense, and ordered gluten network	794:845	a more continuous, dense, and ordered gluten network	794:845	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	8	40	theme	tensile	1302:1308	arg1	strength					1310:1317	tensile strength	1302:1317	tensile strength	1302:1317	The 25A-75B noodle displayed maximum hardness, cohesiveness, chewiness, and tensile strength.					
37330103	0	41	theme	particle	13:20	arg1	distribution					27:38	Wheat starch particle size distribution	0:38	Wheat starch particle size distribution	0:38	Wheat starch particle size distribution regulates the dynamic transition behavior of gluten at different stages of dough mixing.					
37330103	0	42	theme	dough	115:119	arg1	mixing					121:126	dough mixing	115:126	dough mixing	115:126	Wheat starch particle size distribution regulates the dynamic transition behavior of gluten at different stages of dough mixing.					
37330103	2	43	theme	Research	365:372	arg1	results					374:380	Research results	365:380	Research results	365:380	Research results indicated that mixing process induced glutenin macropolymer depolymerization, and promoted the monomeric protein conversion into the polymeric protein.					
37330103	4	44	theme	microscopy	681:690	arg1	images					692:697	Confocal laser scanning microscopy images	657:697	Confocal laser scanning microscopy images	657:697	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	2	45	theme	polymeric	515:523	arg1	protein					525:531	the polymeric protein	511:531	the polymeric protein	511:531	Research results indicated that mixing process induced glutenin macropolymer depolymerization, and promoted the monomeric protein conversion into the polymeric protein.					
37330103	9	46	theme	noodle	1410:1415	arg1	quality					1417:1423	noodle quality	1410:1423	noodle quality	1410:1423	The correlation analysis indicated that starch particle size distribution could influence noodle quality by changing the gluten network.					
37330103	10	47	theme	granule	1560:1566	arg1	distribution					1573:1584	the starch granule size distribution	1549:1584	the starch granule size distribution	1549:1584	The paper can provide theoretical support for regulating dough characteristics by adjusting the starch granule size distribution.					
37330103	6	48	theme	random	1066:1071	arg1	structure					1078:1086	random coil structure	1066:1086	random coil structure	1066:1086	The addition of B-starch increased α-helixes, β-turns, and random coil structure.					
37330103	3	49	theme	gluten	641:646	arg1	protein					648:654	gluten protein	641:654	gluten protein	641:654	Appropriate mixing (9 min) enhanced the interaction between wheat starch with different particle sizes and gluten protein.					
37330103	5	50	theme	gluten	913:918	arg1	network					920:926	a dense gluten network	905:926	a dense gluten network	905:926	The 50A-50B and 25A-75B doughs mixed for 9 min exhibited a dense gluten network, and the arrangement of A-/B-starch granules and gluten was tight and ordered.					
37330103	1	51	theme	aggregative	207:217	arg1	properties					219:228	aggregative properties	207:228	aggregative properties	207:228	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
37330103	4	52	theme	moderate	713:720	arg1	increase					722:729	a moderate increase	711:729	a moderate increase in B-starch content in the dough system	711:769	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	5	53	theme	dense	907:911	arg1	network					920:926	a dense gluten network	905:926	a dense gluten network	905:926	The 50A-50B and 25A-75B doughs mixed for 9 min exhibited a dense gluten network, and the arrangement of A-/B-starch granules and gluten was tight and ordered.					
37330103	6	54	theme	B-starch	1023:1030	arg1	addition					1011:1018	The addition	1007:1018	The addition of B-starch	1007:1030	The addition of B-starch increased α-helixes, β-turns, and random coil structure.					
37330103	4	55	theme	scanning	672:679	arg1	microscopy					681:690	Confocal laser scanning microscopy	657:690	Confocal laser scanning microscopy images	657:697	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	2	56	theme	macropolymer	429:440	arg1	depolymerization					442:457	glutenin macropolymer depolymerization	420:457	glutenin macropolymer depolymerization	420:457	Research results indicated that mixing process induced glutenin macropolymer depolymerization, and promoted the monomeric protein conversion into the polymeric protein.					
37330103	0	57	theme	dynamic	54:60	arg1	behavior					73:80	the dynamic transition behavior	50:80	the dynamic transition behavior of gluten	50:90	Wheat starch particle size distribution regulates the dynamic transition behavior of gluten at different stages of dough mixing.					
37330103	5	58	theme	25A-75B	864:870	arg1	doughs					872:877	The 50A-50B and 25A-75B doughs	848:877	The 50A-50B and 25A-75B doughs mixed for 9 min	848:893	The 50A-50B and 25A-75B doughs mixed for 9 min exhibited a dense gluten network, and the arrangement of A-/B-starch granules and gluten was tight and ordered.					
37330103	1	59	dep	distribution	168:179	arg1	variation					230:238	variation	230:238	variation	230:238	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
37330103	3	60	theme	Appropriate	534:544	arg1	9 min					554:558	9 min	554:558	9 min	554:558	Appropriate mixing (9 min) enhanced the interaction between wheat starch with different particle sizes and gluten protein.					
37330103	3	60	theme	Appropriate	534:544	arg1	mixing					546:551	Appropriate mixing	534:551	Appropriate mixing (9 min)	534:559	Appropriate mixing (9 min) enhanced the interaction between wheat starch with different particle sizes and gluten protein.					
37330103	7	61	theme	Farinographic	1089:1101	arg1	properties					1103:1112	Farinographic properties	1089:1112	Farinographic properties	1089:1112	Farinographic properties indicated that 25A-75B composite flour had the highest dough stability time and the lowest degree of softening.					
37330103	7	62	dep	stability	1175:1183	arg1	time					1185:1188	time	1185:1188	time	1185:1188	Farinographic properties indicated that 25A-75B composite flour had the highest dough stability time and the lowest degree of softening.					
37330103	4	63	theme	dough	758:762	arg1	system					764:769	the dough system	754:769	the dough system	754:769	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	1	64	theme	different	336:344	arg1	sizes					346:350	different sizes	336:350	different sizes	336:350	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
37330103	10	65	theme	theoretical	1479:1489	arg1	support					1491:1497	theoretical support	1479:1497	theoretical support for regulating dough characteristics by adjusting the starch granule size distribution	1479:1584	The paper can provide theoretical support for regulating dough characteristics by adjusting the starch granule size distribution.					
37330103	4	66	theme	gluten	832:837	arg1	network					839:845	a more continuous, dense, and ordered gluten network	794:845	a more continuous, dense, and ordered gluten network	794:845	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	5	67	theme	A-/B-starch	952:962	arg1	granules					964:971	A-/B-starch granules	952:971	A-/B-starch granules	952:971	The 50A-50B and 25A-75B doughs mixed for 9 min exhibited a dense gluten network, and the arrangement of A-/B-starch granules and gluten was tight and ordered.					
37330103	8	68	theme	25A-75B	1230:1236	arg1	noodle					1238:1243	The 25A-75B noodle	1226:1243	The 25A-75B noodle	1226:1243	The 25A-75B noodle displayed maximum hardness, cohesiveness, chewiness, and tensile strength.					
37330103	4	69	theme	Confocal	657:664	arg1	microscopy					681:690	Confocal laser scanning microscopy	657:690	Confocal laser scanning microscopy images	657:697	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	4	70	theme	ordered	824:830	arg1	network					839:845	a more continuous, dense, and ordered gluten network	794:845	a more continuous, dense, and ordered gluten network	794:845	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	4	71	theme	B-starch	734:741	arg1	content					743:749	B-starch content	734:749	B-starch content in the dough system	734:769	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	7	72	theme	lowest	1198:1203	arg1	degree					1205:1210	the lowest degree	1194:1210	the lowest degree of softening	1194:1223	Farinographic properties indicated that 25A-75B composite flour had the highest dough stability time and the lowest degree of softening.					
37330103	1	73	theme	gluten	243:248	arg1	protein					250:256	gluten protein	243:256	gluten protein	243:256	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
37330103	0	74	theme	transition	62:71	arg1	behavior					73:80	the dynamic transition behavior	50:80	the dynamic transition behavior of gluten	50:90	Wheat starch particle size distribution regulates the dynamic transition behavior of gluten at different stages of dough mixing.					
37330103	4	75	theme	laser	666:670	arg1	microscopy					681:690	Confocal laser scanning microscopy	657:690	Confocal laser scanning microscopy images	657:697	Confocal laser scanning microscopy images showed that a moderate increase in B-starch content in the dough system contributed to forming a more continuous, dense, and ordered gluten network.					
37330103	2	76	theme	protein	487:493	arg1	conversion					495:504	the monomeric protein conversion	473:504	the monomeric protein conversion into the polymeric protein	473:531	Research results indicated that mixing process induced glutenin macropolymer depolymerization, and promoted the monomeric protein conversion into the polymeric protein.					
37330103	7	77	theme	softening	1215:1223	arg1	degree					1205:1210	the lowest degree	1194:1210	the lowest degree of softening	1194:1223	Farinographic properties indicated that 25A-75B composite flour had the highest dough stability time and the lowest degree of softening.					
37330103	7	77	theme	softening	1215:1223	arg1	stability					1175:1183	the highest dough stability time and the lowest degree of softening	1157:1223	stability	1175:1183	Farinographic properties indicated that 25A-75B composite flour had the highest dough stability time and the lowest degree of softening.					
37330103	1	78	theme	protein	250:256	arg1	distribution					168:179	the morphology distribution	153:179	the morphology distribution	153:179	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
37330103	1	78	theme	protein	250:256	arg1	structure					192:200	molecular structure	182:200	molecular structure	182:200	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
37330103	1	78	theme	protein	250:256	arg1	properties					219:228	aggregative properties	207:228	aggregative properties	207:228	This study investigated the morphology distribution, molecular structure, and aggregative properties variation of gluten protein during dough mixing stage and interpreted the interaction between starch with different sizes and protein.					
36565825	0	0	theme	rind	71:74	arg1	extract					76:82	watermelon rind extract	60:82	watermelon rind extract	60:82	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	1	1	theme	physicochemical	271:285	arg1	properties					287:296	the physicochemical properties	267:296	the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas	267:425	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	2	2	theme	WRE	551:553	arg1	matrix					562:567	the WRE and CG matrix	547:567	the WRE and CG matrix	547:567	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	2	3	theme	films	661:665	arg1	structure					634:642	the structure	630:642	the structure of the resulting films	630:665	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	3	4	theme	oxygen	822:827	arg1	permeability					790:801	the permeability	786:801	the permeability of water vapor and oxygen	786:827	With increasing amounts of WRE, the mechanical properties of the films were significantly increased, but the permeability of water vapor and oxygen was significantly decreased (p < 0.05).					
36565825	5	5	theme	room	1111:1114	arg1	temperature					1116:1126	room temperature	1111:1126	room temperature	1111:1126	Fresh-cut bananas were stored at room temperature with polyethylene film, CG and CG-WRE.					
36565825	0	6	theme	watermelon	60:69	arg1	extract					76:82	watermelon rind extract	60:82	watermelon rind extract	60:82	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	7	7	theme	novel	1355:1359	arg1	properties					1418:1427	good physicochemical properties	1397:1427	good physicochemical properties	1397:1427	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	7	theme	novel	1355:1359	arg1	material					1383:1390	a novel active food packaging material	1353:1390	a novel active food packaging material	1353:1390	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	7	theme	novel	1355:1359	arg1	potential					1439:1447	great potential	1433:1447	great potential	1433:1447	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	6	8	theme	%	1183:1183	arg1	WRE					1185:1187	4 wt% WRE	1179:1187	4 wt% WRE	1179:1187	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	3	9	theme	water	806:810	arg1	vapor					812:816	water vapor	806:816	water vapor	806:816	With increasing amounts of WRE, the mechanical properties of the films were significantly increased, but the permeability of water vapor and oxygen was significantly decreased (p < 0.05).					
36565825	2	10	theme	resulting	651:659	arg1	films					661:665	the resulting films	647:665	the resulting films	647:665	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	7	11	theme	active	1361:1366	arg1	properties					1418:1427	good physicochemical properties	1397:1427	good physicochemical properties	1397:1427	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	11	theme	active	1361:1366	arg1	material					1383:1390	a novel active food packaging material	1353:1390	a novel active food packaging material	1353:1390	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	11	theme	active	1361:1366	arg1	potential					1439:1447	great potential	1433:1447	great potential	1433:1447	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	6	12	theme	4 wt	1179:1182	arg1	WRE					1185:1187	4 wt% WRE	1179:1187	4 wt% WRE	1179:1187	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	1	13	theme	active	368:373	arg1	substance					375:383	a cross-linker and active substance	349:383	a cross-linker and active substance for the preservation of fresh-cut bananas	349:425	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	1	13	theme	active	368:373	arg1	extract					332:338	watermelon rind extract	316:338	watermelon rind extract (WRE)	316:344	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	3	14	theme	vapor	812:816	arg1	permeability					790:801	the permeability	786:801	the permeability of water vapor and oxygen	786:827	With increasing amounts of WRE, the mechanical properties of the films were significantly increased, but the permeability of water vapor and oxygen was significantly decreased (p < 0.05).					
36565825	2	15	theme	hydrogen	591:598	arg1	interactions					605:616	intermolecular hydrogen bond interactions	576:616	intermolecular hydrogen bond interactions	576:616	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	1	16	theme	guar	237:240	arg1	GG					247:248	GG	247:248	GG	247:248	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	1	16	theme	guar	237:240	arg1	gum					242:244	guar gum	237:244	guar gum (GG)	237:249	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	2	17	theme	Fourier	443:449	arg1	diffraction					490:500	X-ray diffraction	484:500	X-ray diffraction	484:500	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	2	17	theme	Fourier	443:449	arg1	results					432:438	The results	428:438	The results of Fourier transform infrared spectroscopy	428:481	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	2	17	theme	Fourier	443:449	arg1	microscopy					524:533	scanning electron microscopy	506:533	scanning electron microscopy	506:533	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	1	18	theme	film	305:308	arg1	properties					287:296	the physicochemical properties	267:296	the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas	267:425	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	2	19	theme	intermolecular	576:589	arg1	interactions					605:616	intermolecular hydrogen bond interactions	576:616	intermolecular hydrogen bond interactions	576:616	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	4	20	theme	film	974:977	arg1	activity					948:955	the DPPH radical scavenging activity	920:955	the DPPH radical scavenging activity of the composite film	920:977	Notably, when the amount of extract reached 4 wt%, the DPPH radical scavenging activity of the composite film significantly increased to 83.24 %, and the antibacterial activity also reached its highest value.					
36565825	6	21	theme	total	1266:1270	arg1	solids					1280:1285	total soluble solids	1266:1285	total soluble solids	1266:1285	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	5	22	theme	polyethylene	1133:1144	arg1	film					1146:1149	polyethylene film	1133:1149	polyethylene film	1133:1149	Fresh-cut bananas were stored at room temperature with polyethylene film, CG and CG-WRE.					
36565825	6	23	dep	appearance	1226:1235	arg1	content					1287:1293	content	1287:1293	content	1287:1293	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	7	24	theme	foods	1477:1481	arg1	life					1469:1472	the shelf life	1459:1472	the shelf life of foods	1459:1481	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	6	25	from	changes	1215:1221	arg1	appearance					1226:1235	appearance	1226:1235	appearance	1226:1235	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	6	25	from	changes	1215:1221	arg1	color					1256:1260	color	1256:1260	color	1256:1260	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	6	25	from	changes	1215:1221	arg1	weight					1248:1253	weight	1248:1253	weight	1248:1253	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	6	25	from	changes	1215:1221	arg1	firmness					1238:1245	firmness	1238:1245	firmness	1238:1245	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	6	25	from	changes	1215:1221	arg1	solids					1280:1285	total soluble solids	1266:1285	total soluble solids	1266:1285	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	4	26	theme	scavenging	937:946	arg1	activity					948:955	the DPPH radical scavenging activity	920:955	the DPPH radical scavenging activity of the composite film	920:977	Notably, when the amount of extract reached 4 wt%, the DPPH radical scavenging activity of the composite film significantly increased to 83.24 %, and the antibacterial activity also reached its highest value.					
36565825	1	27	theme	watermelon	316:325	arg1	substance					375:383	a cross-linker and active substance	349:383	a cross-linker and active substance for the preservation of fresh-cut bananas	349:425	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	1	27	theme	watermelon	316:325	arg1	WRE					341:343	WRE	341:343	WRE	341:343	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	1	27	theme	watermelon	316:325	arg1	extract					332:338	watermelon rind extract	316:338	watermelon rind extract (WRE)	316:344	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	2	28	theme	CG	559:560	arg1	matrix					562:567	the WRE and CG matrix	547:567	the WRE and CG matrix	547:567	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	1	29	theme	study	176:180	arg1	objective					158:166	The objective	154:166	The objective of this study	154:180	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	4	30	theme	radical	929:935	arg1	activity					948:955	the DPPH radical scavenging activity	920:955	the DPPH radical scavenging activity of the composite film	920:977	Notably, when the amount of extract reached 4 wt%, the DPPH radical scavenging activity of the composite film significantly increased to 83.24 %, and the antibacterial activity also reached its highest value.					
36565825	7	31	theme	packaging	1373:1381	arg1	properties					1418:1427	good physicochemical properties	1397:1427	good physicochemical properties	1397:1427	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	31	theme	packaging	1373:1381	arg1	material					1383:1390	a novel active food packaging material	1353:1390	a novel active food packaging material	1353:1390	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	31	theme	packaging	1373:1381	arg1	potential					1439:1447	great potential	1433:1447	great potential	1433:1447	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	32	contain	has	1393:1395	arg2	potential					1439:1447	great potential	1433:1447	great potential	1433:1447	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	32	contain	has	1393:1395	arg2	properties					1418:1427	good physicochemical properties	1397:1427	good physicochemical properties	1397:1427	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	32	contain	has	1393:1395	arg1	CG-WRE					1343:1348	CG-WRE	1343:1348	CG-WRE	1343:1348	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	32	contain	has	1393:1395	arg2	material					1383:1390	a novel active food packaging material	1353:1390	a novel active food packaging material	1353:1390	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	0	33	theme	packaging	22:30	arg1	Development					0:10	Development	0:10	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.	0:152	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	2	34	dep	Fourier	443:449	arg1	transform					451:459	transform	451:459	transform infrared spectroscopy	451:481	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	1	35	theme	fresh-cut	409:417	arg1	bananas					419:425	fresh-cut bananas	409:425	fresh-cut bananas	409:425	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	0	36	theme	active	15:20	arg1	packaging					22:30	active packaging	15:30	active packaging with chitosan, guar gum and watermelon rind extract	15:82	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	2	37	theme	X-ray	484:488	arg1	diffraction					490:500	X-ray diffraction	484:500	X-ray diffraction	484:500	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	2	37	theme	X-ray	484:488	arg1	results					432:438	The results	428:438	The results of Fourier transform infrared spectroscopy	428:481	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	1	38	theme	rind	327:330	arg1	substance					375:383	a cross-linker and active substance	349:383	a cross-linker and active substance for the preservation of fresh-cut bananas	349:425	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	1	38	theme	rind	327:330	arg1	WRE					341:343	WRE	341:343	WRE	341:343	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	1	38	theme	rind	327:330	arg1	extract					332:338	watermelon rind extract	316:338	watermelon rind extract (WRE)	316:344	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	4	39	theme	antibacterial	1023:1035	arg1	activity					1037:1044	the antibacterial activity	1019:1044	the antibacterial activity	1019:1044	Notably, when the amount of extract reached 4 wt%, the DPPH radical scavenging activity of the composite film significantly increased to 83.24 %, and the antibacterial activity also reached its highest value.					
36565825	6	40	theme	bananas	1308:1314	arg1	weight					1248:1253	weight	1248:1253	weight	1248:1253	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	6	40	theme	bananas	1308:1314	arg1	appearance					1226:1235	appearance	1226:1235	appearance	1226:1235	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	6	40	theme	bananas	1308:1314	arg1	solids					1280:1285	total soluble solids	1266:1285	total soluble solids	1266:1285	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	6	40	theme	bananas	1308:1314	arg1	color					1256:1260	color	1256:1260	color	1256:1260	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	6	40	theme	bananas	1308:1314	arg1	firmness					1238:1245	firmness	1238:1245	firmness	1238:1245	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	3	41	theme	increasing	686:695	arg1	amounts					697:703	increasing amounts	686:703	increasing amounts of WRE	686:710	With increasing amounts of WRE, the mechanical properties of the films were significantly increased, but the permeability of water vapor and oxygen was significantly decreased (p < 0.05).					
36565825	3	41	theme	increasing	686:695	arg1	WRE					708:710	WRE	708:710	WRE	708:710	With increasing amounts of WRE, the mechanical properties of the films were significantly increased, but the permeability of water vapor and oxygen was significantly decreased (p < 0.05).					
36565825	0	42	theme	improvement	131:141	arg1	mechanism					143:151	performance improvement mechanism	119:151	performance improvement mechanism	119:151	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	6	43	with	CG	1171:1172	arg1	WRE					1185:1187	4 wt% WRE	1179:1187	4 wt% WRE	1179:1187	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	6	44	theme	fresh-cut	1298:1306	arg1	bananas					1308:1314	fresh-cut bananas	1298:1314	fresh-cut bananas	1298:1314	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	0	45	theme	performance	119:129	arg1	mechanism					143:151	performance improvement mechanism	119:151	performance improvement mechanism	119:151	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	7	46	theme	physicochemical	1402:1416	arg1	properties					1418:1427	good physicochemical properties	1397:1427	good physicochemical properties	1397:1427	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	46	theme	physicochemical	1402:1416	arg1	material					1383:1390	a novel active food packaging material	1353:1390	a novel active food packaging material	1353:1390	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	46	theme	physicochemical	1402:1416	arg1	potential					1439:1447	great potential	1433:1447	great potential	1433:1447	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	2	47	dep	formed	569:574	arg1	made					625:628	made	625:628	made the structure of the resulting films more compact	625:678	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	1	48	theme	bananas	419:425	arg1	preservation					393:404	the preservation	389:404	the preservation of fresh-cut bananas	389:425	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	1	49	theme	film	196:199	arg1	matrix					201:206	a film matrix	194:206	a film matrix containing chitosan (CS) and guar gum (GG)	194:249	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	0	50	theme	guar	47:50	arg1	gum					52:54	guar gum	47:54	guar gum	47:54	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	3	51	theme	mechanical	717:726	arg1	properties					728:737	the mechanical properties	713:737	the mechanical properties of the films	713:750	With increasing amounts of WRE, the mechanical properties of the films were significantly increased, but the permeability of water vapor and oxygen was significantly decreased (p < 0.05).					
36565825	6	52	theme	soluble	1272:1278	arg1	solids					1280:1285	total soluble solids	1266:1285	total soluble solids	1266:1285	The CG with 4 wt% WRE effectively inhibited the changes in appearance, firmness, weight, color and total soluble solids content of fresh-cut bananas during storage.					
36565825	1	53	contain	containing	208:217	arg2	gum					242:244	guar gum	237:244	guar gum (GG)	237:249	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	1	53	contain	containing	208:217	arg1	matrix					201:206	a film matrix	194:206	a film matrix containing chitosan (CS) and guar gum (GG)	194:249	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	1	53	contain	containing	208:217	arg2	GG					247:248	GG	247:248	GG	247:248	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	1	53	contain	containing	208:217	arg2	CS					229:230	CS	229:230	CS	229:230	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	1	53	contain	containing	208:217	arg2	chitosan					219:226	chitosan	219:226	chitosan (CS)	219:231	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	2	54	dep	transform	451:459	arg1	infrared					461:468	infrared	461:468	transform infrared spectroscopy	451:481	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	3	55	theme	WRE	708:710	arg1	amounts					697:703	increasing amounts	686:703	increasing amounts of WRE	686:710	With increasing amounts of WRE, the mechanical properties of the films were significantly increased, but the permeability of water vapor and oxygen was significantly decreased (p < 0.05).					
36565825	3	55	theme	WRE	708:710	arg1	WRE					708:710	WRE	708:710	WRE	708:710	With increasing amounts of WRE, the mechanical properties of the films were significantly increased, but the permeability of water vapor and oxygen was significantly decreased (p < 0.05).					
36565825	2	56	theme	electron	515:522	arg1	results					432:438	The results	428:438	The results of Fourier transform infrared spectroscopy	428:481	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	2	56	theme	electron	515:522	arg1	microscopy					524:533	scanning electron microscopy	506:533	scanning electron microscopy	506:533	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	3	57	dep	decreased	847:855	arg1	p < 0.05					858:865	p < 0.05	858:865	p < 0.05	858:865	With increasing amounts of WRE, the mechanical properties of the films were significantly increased, but the permeability of water vapor and oxygen was significantly decreased (p < 0.05).					
36565825	7	58	theme	great	1433:1437	arg1	properties					1418:1427	good physicochemical properties	1397:1427	good physicochemical properties	1397:1427	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	58	theme	great	1433:1437	arg1	potential					1439:1447	great potential	1433:1447	great potential	1433:1447	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	58	theme	great	1433:1437	arg1	material					1383:1390	a novel active food packaging material	1353:1390	a novel active food packaging material	1353:1390	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	5	59	theme	Fresh-cut	1078:1086	arg1	bananas					1088:1094	Fresh-cut bananas	1078:1094	Fresh-cut bananas	1078:1094	Fresh-cut bananas were stored at room temperature with polyethylene film, CG and CG-WRE.					
36565825	2	60	theme	scanning	506:513	arg1	results					432:438	The results	428:438	The results of Fourier transform infrared spectroscopy	428:481	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	2	60	theme	scanning	506:513	arg1	microscopy					524:533	scanning electron microscopy	506:533	scanning electron microscopy	506:533	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	7	61	theme	good	1397:1400	arg1	properties					1418:1427	good physicochemical properties	1397:1427	good physicochemical properties	1397:1427	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	61	theme	good	1397:1400	arg1	material					1383:1390	a novel active food packaging material	1353:1390	a novel active food packaging material	1353:1390	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	61	theme	good	1397:1400	arg1	potential					1439:1447	great potential	1433:1447	great potential	1433:1447	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	2	62	theme	bond	600:603	arg1	interactions					605:616	intermolecular hydrogen bond interactions	576:616	intermolecular hydrogen bond interactions	576:616	The results of Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy showed that the WRE and CG matrix formed intermolecular hydrogen bond interactions, which made the structure of the resulting films more compact.					
36565825	0	63	with	packaging	22:30	arg1	chitosan					37:44	chitosan	37:44	chitosan	37:44	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	0	63	with	packaging	22:30	arg1	gum					52:54	guar gum	47:54	guar gum	47:54	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	0	63	with	packaging	22:30	arg1	extract					76:82	watermelon rind extract	60:82	watermelon rind extract	60:82	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	3	64	theme	films	746:750	arg1	properties					728:737	the mechanical properties	713:737	the mechanical properties of the films	713:750	With increasing amounts of WRE, the mechanical properties of the films were significantly increased, but the permeability of water vapor and oxygen was significantly decreased (p < 0.05).					
36565825	4	65	theme	extract	897:903	arg1	extract					897:903	extract	897:903	extract	897:903	Notably, when the amount of extract reached 4 wt%, the DPPH radical scavenging activity of the composite film significantly increased to 83.24 %, and the antibacterial activity also reached its highest value.					
36565825	4	65	theme	extract	897:903	arg1	amount					887:892	the amount	883:892	the amount of extract	883:903	Notably, when the amount of extract reached 4 wt%, the DPPH radical scavenging activity of the composite film significantly increased to 83.24 %, and the antibacterial activity also reached its highest value.					
36565825	1	66	theme	cross-linker	351:362	arg1	substance					375:383	a cross-linker and active substance	349:383	a cross-linker and active substance for the preservation of fresh-cut bananas	349:425	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	1	66	theme	cross-linker	351:362	arg1	extract					332:338	watermelon rind extract	316:338	watermelon rind extract (WRE)	316:344	The objective of this study was to make a film matrix containing chitosan (CS) and guar gum (GG), and to improve the physicochemical properties of the film using watermelon rind extract (WRE) as a cross-linker and active substance for the preservation of fresh-cut bananas.					
36565825	4	67	theme	highest	1063:1069	arg1	value					1071:1075	its highest value	1059:1075	its highest value	1059:1075	Notably, when the amount of extract reached 4 wt%, the DPPH radical scavenging activity of the composite film significantly increased to 83.24 %, and the antibacterial activity also reached its highest value.					
36565825	7	68	theme	shelf	1463:1467	arg1	life					1469:1472	the shelf life	1459:1472	the shelf life of foods	1459:1481	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	4	69	theme	DPPH	924:927	arg1	activity					948:955	the DPPH radical scavenging activity	920:955	the DPPH radical scavenging activity of the composite film	920:977	Notably, when the amount of extract reached 4 wt%, the DPPH radical scavenging activity of the composite film significantly increased to 83.24 %, and the antibacterial activity also reached its highest value.					
36565825	7	70	theme	food	1368:1371	arg1	properties					1418:1427	good physicochemical properties	1397:1427	good physicochemical properties	1397:1427	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	70	theme	food	1368:1371	arg1	material					1383:1390	a novel active food packaging material	1353:1390	a novel active food packaging material	1353:1390	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	7	70	theme	food	1368:1371	arg1	potential					1439:1447	great potential	1433:1447	great potential	1433:1447	Therefore, CG-WRE as a novel active food packaging material, has good physicochemical properties and great potential to extend the shelf life of foods.					
36565825	0	71	dep	Development	0:10	arg1	application					103:113	application	103:113	application	103:113	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	0	71	dep	Development	0:10	arg1	Characterization					85:100	Characterization	85:100	Characterization	85:100	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	0	71	dep	Development	0:10	arg1	mechanism					143:151	performance improvement mechanism	119:151	performance improvement mechanism	119:151	Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism.					
36565825	4	72	theme	composite	964:972	arg1	film					974:977	the composite film	960:977	the composite film	960:977	Notably, when the amount of extract reached 4 wt%, the DPPH radical scavenging activity of the composite film significantly increased to 83.24 %, and the antibacterial activity also reached its highest value.					
36027986	7	0	theme	distinct	1058:1065	arg1	color					1067:1071	distinct color	1058:1071	distinct color to the film	1058:1083	Increased MPPE concentration showed a decline in transparency (%) and lightness (L*) resulting into distinct color to the film.					
36027986	8	1	theme	Structural	1086:1095	arg1	compactness					1097:1107	Structural compactness	1086:1107	Structural compactness	1086:1107	Structural compactness and integrity of the films were confirmed by SEM and XRD patterns.					
36027986	4	2	theme	Decreased	554:562	arg1	ζ-potential					564:574	Decreased ζ-potential	554:574	Decreased ζ-potential	554:574	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	13	3	theme	food	1749:1752	arg1	coatings					1754:1761	food coatings	1749:1761	food coatings	1749:1761	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	13	3	theme	food	1749:1752	arg1	films					1726:1730	prepared bioactive films	1707:1730	prepared bioactive films	1707:1730	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	1	4	theme	different	272:280	arg1	concentrations					282:295	different concentrations	272:295	different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content)	272:363	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	8	5	theme	XRD	1162:1164	arg1	patterns					1166:1173	XRD patterns	1162:1173	XRD patterns	1162:1173	Structural compactness and integrity of the films were confirmed by SEM and XRD patterns.					
36027986	2	6	used	used	407:410	arg2	film					398:401	The extract-free film	381:401	The extract-free film	381:401	The extract-free film was used as a control.					
36027986	2	6	used	used	407:410	arg2	control					417:423	a control	415:423	a control	415:423	The extract-free film was used as a control.					
36027986	4	7	theme	rheological	635:645	arg1	properties					647:656	improved rheological properties	626:656	improved rheological properties of MPPE	626:664	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	6	8	from	increase	806:813	arg1	concentration					823:835	MPPE concentration	818:835	MPPE concentration	818:835	An increase in MPPE concentration exhibited less hardness, more extensibility, and an excellent barrier to water vapor permeability than the control film.					
36027986	10	9	theme	covalent	1369:1376	arg1	links					1378:1382	covalent links	1369:1382	covalent links	1369:1382	The interaction patterns between starch-casein composite and MPPE bioactives indicated the development of covalent links.					
36027986	4	10	dep	tension	588:594	arg1	dispersion					685:694	added film-forming dispersion	666:694	added film-forming dispersion	666:694	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	1	11	theme	bioactive	194:202	arg1	films					204:208	Starch-casein composite (solid matter content) based bioactive films	141:208	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content)	141:363	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	8	12	theme	films	1130:1134	arg1	integrity					1113:1121	integrity	1113:1121	integrity	1113:1121	Structural compactness and integrity of the films were confirmed by SEM and XRD patterns.					
36027986	8	12	theme	films	1130:1134	arg1	compactness					1097:1107	Structural compactness	1086:1107	Structural compactness	1086:1107	Structural compactness and integrity of the films were confirmed by SEM and XRD patterns.					
36027986	4	13	theme	increased	597:605	arg1	size					616:619	increased particle size	597:619	increased particle size	597:619	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	1	14	theme	matter	349:354	arg1	content					356:362	base solid matter content	338:362	base solid matter content	338:362	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	6	15	theme	water	910:914	arg1	permeability					922:933	water vapor permeability	910:933	water vapor permeability	910:933	An increase in MPPE concentration exhibited less hardness, more extensibility, and an excellent barrier to water vapor permeability than the control film.					
36027986	1	16	theme	micellar	228:235	arg1	extract					254:260	micellar pomegranate peel extract	228:260	micellar pomegranate peel extract (MPPE)	228:267	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	1	16	theme	micellar	228:235	arg1	MPPE					263:266	MPPE	263:266	MPPE	263:266	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	13	17	theme	bioactive	1716:1724	arg1	coatings					1754:1761	food coatings	1749:1761	food coatings	1749:1761	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	13	17	theme	bioactive	1716:1724	arg1	films					1726:1730	prepared bioactive films	1707:1730	prepared bioactive films	1707:1730	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	9	18	theme	Improved	1176:1183	arg1	interaction					1196:1206	Improved functional interaction	1176:1206	Improved functional interaction	1176:1206	Improved functional interaction and thermal reliability of bioactive films were noted.					
36027986	7	19	theme	Increased	958:966	arg1	concentration					973:985	Increased MPPE concentration	958:985	Increased MPPE concentration	958:985	Increased MPPE concentration showed a decline in transparency (%) and lightness (L*) resulting into distinct color to the film.					
36027986	11	20	with	bioactivities	1395:1407	arg1	release					1423:1429	the slow release	1414:1429	the slow release of bioactives in hydroalcoholic environment	1414:1473	Excellent bioactivities with the slow release of bioactives in hydroalcoholic environment, confirmed by the kinetic study.					
36027986	1	21	theme	Starch-casein	141:153	arg1	content					179:185	solid matter content	166:185	solid matter content	166:185	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	1	21	theme	Starch-casein	141:153	arg1	composite					155:163	Starch-casein composite	141:163	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content)	141:363	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	11	22	theme	hydroalcoholic	1448:1461	arg1	environment					1463:1473	hydroalcoholic environment	1448:1473	hydroalcoholic environment	1448:1473	Excellent bioactivities with the slow release of bioactives in hydroalcoholic environment, confirmed by the kinetic study.					
36027986	0	23	theme	films	75:79	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of taro starch-casein composite bioactive films functionalized by micellar pomegranate peel extract (MPPE).					
36027986	0	23	theme	films	75:79	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of taro starch-casein composite bioactive films functionalized by micellar pomegranate peel extract (MPPE).					
36027986	9	24	theme	thermal	1212:1218	arg1	reliability					1220:1230	thermal reliability	1212:1230	thermal reliability	1212:1230	Improved functional interaction and thermal reliability of bioactive films were noted.					
36027986	13	25	theme	improved	1665:1672	arg1	strength					1690:1697	improved physicochemical strength	1665:1697	improved physicochemical strength	1665:1697	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	4	26	theme	added	666:670	arg1	dispersion					685:694	added film-forming dispersion	666:694	added film-forming dispersion	666:694	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	11	27	theme	kinetic	1493:1499	arg1	study					1501:1505	the kinetic study	1489:1505	the kinetic study	1489:1505	Excellent bioactivities with the slow release of bioactives in hydroalcoholic environment, confirmed by the kinetic study.					
36027986	5	28	theme	physical	731:738	arg1	properties					740:749	all the physical properties	723:749	all the physical properties of bioactive films	723:768	Among all the physical properties of bioactive films, only contact angle was reduced.					
36027986	1	29	theme	matter	172:177	arg1	content					179:185	solid matter content	166:185	solid matter content	166:185	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	1	29	theme	matter	172:177	arg1	composite					155:163	Starch-casein composite	141:163	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content)	141:363	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	0	30	theme	pomegranate	108:118	arg1	MPPE					134:137	MPPE	134:137	MPPE	134:137	Development and characterization of taro starch-casein composite bioactive films functionalized by micellar pomegranate peel extract (MPPE).					
36027986	0	30	theme	pomegranate	108:118	arg1	extract					125:131	micellar pomegranate peel extract	99:131	micellar pomegranate peel extract (MPPE)	99:138	Development and characterization of taro starch-casein composite bioactive films functionalized by micellar pomegranate peel extract (MPPE).					
36027986	9	31	theme	films	1245:1249	arg1	interaction					1196:1206	Improved functional interaction	1176:1206	Improved functional interaction	1176:1206	Improved functional interaction and thermal reliability of bioactive films were noted.					
36027986	9	31	theme	films	1245:1249	arg1	reliability					1220:1230	thermal reliability	1212:1230	thermal reliability	1212:1230	Improved functional interaction and thermal reliability of bioactive films were noted.					
36027986	12	32	theme	Remarkable	1508:1517	arg1	effect					1533:1538	Remarkable antibacterial effect	1508:1538	Remarkable antibacterial effect	1508:1538	Remarkable antibacterial effect was noted against E. coli and S. aureus by the films.					
36027986	13	33	from	concentration	1618:1630	arg1	film					1653:1656	bioactive film	1643:1656	bioactive film	1643:1656	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	1	34	theme	base	338:341	arg1	content					356:362	base solid matter content	338:362	base solid matter content	338:362	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	5	35	theme	only	771:774	arg1	angle					784:788	only contact angle	771:788	only contact angle	771:788	Among all the physical properties of bioactive films, only contact angle was reduced.					
36027986	11	36	theme	bioactives	1434:1443	arg1	release					1423:1429	the slow release	1414:1429	the slow release of bioactives in hydroalcoholic environment	1414:1473	Excellent bioactivities with the slow release of bioactives in hydroalcoholic environment, confirmed by the kinetic study.					
36027986	2	37	theme	extract-free	385:396	arg1	control					417:423	a control	415:423	a control	415:423	The extract-free film was used as a control.					
36027986	2	37	theme	extract-free	385:396	arg1	film					398:401	The extract-free film	381:401	The extract-free film	381:401	The extract-free film was used as a control.					
36027986	0	38	theme	taro	36:39	arg1	films					75:79	taro starch-casein composite bioactive films	36:79	taro starch-casein composite bioactive films	36:79	Development and characterization of taro starch-casein composite bioactive films functionalized by micellar pomegranate peel extract (MPPE).					
36027986	11	39	from	release	1423:1429	arg1	environment					1463:1473	hydroalcoholic environment	1448:1473	hydroalcoholic environment	1448:1473	Excellent bioactivities with the slow release of bioactives in hydroalcoholic environment, confirmed by the kinetic study.					
36027986	5	40	theme	films	764:768	arg1	properties					740:749	all the physical properties	723:749	all the physical properties of bioactive films	723:768	Among all the physical properties of bioactive films, only contact angle was reduced.					
36027986	3	41	theme	bioactive	430:438	arg1	films					440:444	The bioactive films	426:444	The bioactive films	426:444	The bioactive films were characterized for physicochemical, mechanical, barrier, structural, thermal, and bioactive properties.					
36027986	1	42	theme	pomegranate	237:247	arg1	extract					254:260	micellar pomegranate peel extract	228:260	micellar pomegranate peel extract (MPPE)	228:267	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	1	42	theme	pomegranate	237:247	arg1	MPPE					263:266	MPPE	263:266	MPPE	263:266	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	7	43	from	decline	996:1002	arg1	%					1021:1021	%	1021:1021	%	1021:1021	Increased MPPE concentration showed a decline in transparency (%) and lightness (L*) resulting into distinct color to the film.					
36027986	7	43	from	decline	996:1002	arg1	lightness					1028:1036	lightness	1028:1036	lightness (L*)	1028:1041	Increased MPPE concentration showed a decline in transparency (%) and lightness (L*) resulting into distinct color to the film.					
36027986	7	43	from	decline	996:1002	arg1	transparency					1007:1018	transparency	1007:1018	transparency (%)	1007:1022	Increased MPPE concentration showed a decline in transparency (%) and lightness (L*) resulting into distinct color to the film.					
36027986	7	43	from	decline	996:1002	arg1	*					1040:1040	L*	1039:1040	L*	1039:1040	Increased MPPE concentration showed a decline in transparency (%) and lightness (L*) resulting into distinct color to the film.					
36027986	0	44	theme	bioactive	65:73	arg1	films					75:79	taro starch-casein composite bioactive films	36:79	taro starch-casein composite bioactive films	36:79	Development and characterization of taro starch-casein composite bioactive films functionalized by micellar pomegranate peel extract (MPPE).					
36027986	4	45	theme	surface	580:586	arg1	FFD					697:699	FFD	697:699	FFD	697:699	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	4	45	theme	surface	580:586	arg1	tension					588:594	surface tension	580:594	surface tension	580:594	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	10	46	theme	starch-casein	1296:1308	arg1	bioactives					1329:1338	starch-casein composite and MPPE bioactives	1296:1338	starch-casein composite and MPPE bioactives	1296:1338	The interaction patterns between starch-casein composite and MPPE bioactives indicated the development of covalent links.					
36027986	7	47	theme	L	1039:1039	arg1	lightness					1028:1036	lightness	1028:1036	lightness (L*)	1028:1041	Increased MPPE concentration showed a decline in transparency (%) and lightness (L*) resulting into distinct color to the film.					
36027986	7	47	theme	L	1039:1039	arg1	*					1040:1040	L*	1039:1040	L*	1039:1040	Increased MPPE concentration showed a decline in transparency (%) and lightness (L*) resulting into distinct color to the film.					
36027986	0	48	theme	composite	55:63	arg1	films					75:79	taro starch-casein composite bioactive films	36:79	taro starch-casein composite bioactive films	36:79	Development and characterization of taro starch-casein composite bioactive films functionalized by micellar pomegranate peel extract (MPPE).					
36027986	10	49	theme	links	1378:1382	arg1	development					1354:1364	the development	1350:1364	the development of covalent links	1350:1382	The interaction patterns between starch-casein composite and MPPE bioactives indicated the development of covalent links.					
36027986	1	50	theme	based	188:192	arg1	films					204:208	Starch-casein composite (solid matter content) based bioactive films	141:208	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content)	141:363	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	4	51	theme	improved	626:633	arg1	properties					647:656	improved rheological properties	626:656	improved rheological properties of MPPE	626:664	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	6	52	theme	vapor	916:920	arg1	permeability					922:933	water vapor permeability	910:933	water vapor permeability	910:933	An increase in MPPE concentration exhibited less hardness, more extensibility, and an excellent barrier to water vapor permeability than the control film.					
36027986	4	53	theme	particle	607:614	arg1	size					616:619	increased particle size	597:619	increased particle size	597:619	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	13	54	theme	prepared	1707:1714	arg1	coatings					1754:1761	food coatings	1749:1761	food coatings	1749:1761	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	13	54	theme	prepared	1707:1714	arg1	films					1726:1730	prepared bioactive films	1707:1730	prepared bioactive films	1707:1730	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	3	55	theme	bioactive	532:540	arg1	properties					542:551	bioactive properties	532:551	bioactive properties	532:551	The bioactive films were characterized for physicochemical, mechanical, barrier, structural, thermal, and bioactive properties.					
36027986	6	56	theme	excellent	889:897	arg1	barrier					899:905	an excellent barrier	886:905	an excellent barrier to water vapor permeability	886:933	An increase in MPPE concentration exhibited less hardness, more extensibility, and an excellent barrier to water vapor permeability than the control film.					
36027986	4	57	theme	film-forming	672:683	arg1	dispersion					685:694	added film-forming dispersion	666:694	added film-forming dispersion	666:694	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	9	58	theme	functional	1185:1194	arg1	interaction					1196:1206	Improved functional interaction	1176:1206	Improved functional interaction	1176:1206	Improved functional interaction and thermal reliability of bioactive films were noted.					
36027986	7	59	theme	MPPE	968:971	arg1	concentration					973:985	Increased MPPE concentration	958:985	Increased MPPE concentration	958:985	Increased MPPE concentration showed a decline in transparency (%) and lightness (L*) resulting into distinct color to the film.					
36027986	1	60	theme	composite	155:163	arg1	films					204:208	Starch-casein composite (solid matter content) based bioactive films	141:208	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content)	141:363	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	6	61	theme	control	944:950	arg1	film					952:955	the control film	940:955	the control film	940:955	An increase in MPPE concentration exhibited less hardness, more extensibility, and an excellent barrier to water vapor permeability than the control film.					
36027986	0	62	theme	micellar	99:106	arg1	MPPE					134:137	MPPE	134:137	MPPE	134:137	Development and characterization of taro starch-casein composite bioactive films functionalized by micellar pomegranate peel extract (MPPE).					
36027986	0	62	theme	micellar	99:106	arg1	extract					125:131	micellar pomegranate peel extract	99:131	micellar pomegranate peel extract (MPPE)	99:138	Development and characterization of taro starch-casein composite bioactive films functionalized by micellar pomegranate peel extract (MPPE).					
36027986	1	63	theme	100 wt	314:319	arg1	%					320:320	10, 25, 50, and 100 wt%	298:320	10, 25, 50, and 100 wt%	298:320	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	1	64	theme	solid	166:170	arg1	content					179:185	solid matter content	166:185	solid matter content	166:185	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	1	64	theme	solid	166:170	arg1	composite					155:163	Starch-casein composite	141:163	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content)	141:363	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	1	65	dep	concentrations	282:295	arg1	%					320:320	10, 25, 50, and 100 wt%	298:320	10, 25, 50, and 100 wt%	298:320	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	13	66	theme	physicochemical	1674:1688	arg1	strength					1690:1697	improved physicochemical strength	1665:1697	improved physicochemical strength	1665:1697	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	4	67	theme	MPPE	661:664	arg1	FFD					697:699	FFD	697:699	FFD	697:699	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	4	67	theme	MPPE	661:664	arg1	properties					647:656	improved rheological properties	626:656	improved rheological properties of MPPE	626:664	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	4	67	theme	MPPE	661:664	arg1	size					616:619	increased particle size	597:619	increased particle size	597:619	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	4	67	theme	MPPE	661:664	arg1	ζ-potential					564:574	Decreased ζ-potential	554:574	Decreased ζ-potential	554:574	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	4	67	theme	MPPE	661:664	arg1	tension					588:594	surface tension	580:594	surface tension	580:594	Decreased ζ-potential and surface tension, increased particle size, and improved rheological properties of MPPE added film-forming dispersion (FFD) were recorded.					
36027986	0	68	theme	peel	120:123	arg1	MPPE					134:137	MPPE	134:137	MPPE	134:137	Development and characterization of taro starch-casein composite bioactive films functionalized by micellar pomegranate peel extract (MPPE).					
36027986	0	68	theme	peel	120:123	arg1	extract					125:131	micellar pomegranate peel extract	99:131	micellar pomegranate peel extract (MPPE)	99:138	Development and characterization of taro starch-casein composite bioactive films functionalized by micellar pomegranate peel extract (MPPE).					
36027986	9	69	theme	bioactive	1235:1243	arg1	films					1245:1249	bioactive films	1235:1249	bioactive films	1235:1249	Improved functional interaction and thermal reliability of bioactive films were noted.					
36027986	12	70	theme	antibacterial	1519:1531	arg1	effect					1533:1538	Remarkable antibacterial effect	1508:1538	Remarkable antibacterial effect	1508:1538	Remarkable antibacterial effect was noted against E. coli and S. aureus by the films.					
36027986	13	71	used	used	1741:1744	arg2	films					1726:1730	prepared bioactive films	1707:1730	prepared bioactive films	1707:1730	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	13	71	used	used	1741:1744	arg2	coatings					1754:1761	food coatings	1749:1761	food coatings	1749:1761	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	13	72	theme	bioactive	1643:1651	arg1	film					1653:1656	bioactive film	1643:1656	bioactive film	1643:1656	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	11	73	theme	Excellent	1385:1393	arg1	bioactivities					1395:1407	Excellent bioactivities	1385:1407	Excellent bioactivities	1385:1407	Excellent bioactivities with the slow release of bioactives in hydroalcoholic environment, confirmed by the kinetic study.					
36027986	1	74	theme	solid	343:347	arg1	content					356:362	base solid matter content	338:362	base solid matter content	338:362	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	5	75	theme	contact	776:782	arg1	angle					784:788	only contact angle	771:788	only contact angle	771:788	Among all the physical properties of bioactive films, only contact angle was reduced.					
36027986	11	76	theme	slow	1418:1421	arg1	release					1423:1429	the slow release	1414:1429	the slow release of bioactives in hydroalcoholic environment	1414:1473	Excellent bioactivities with the slow release of bioactives in hydroalcoholic environment, confirmed by the kinetic study.					
36027986	0	77	theme	starch-casein	41:53	arg1	films					75:79	taro starch-casein composite bioactive films	36:79	taro starch-casein composite bioactive films	36:79	Development and characterization of taro starch-casein composite bioactive films functionalized by micellar pomegranate peel extract (MPPE).					
36027986	6	78	theme	MPPE	818:821	arg1	concentration					823:835	MPPE concentration	818:835	MPPE concentration	818:835	An increase in MPPE concentration exhibited less hardness, more extensibility, and an excellent barrier to water vapor permeability than the control film.					
36027986	13	79	theme	MPPE	1635:1638	arg1	concentration					1618:1630	the concentration	1614:1630	the concentration of MPPE in bioactive film	1614:1656	Overall, increasing the concentration of MPPE in bioactive film showed improved physicochemical strength; hence, prepared bioactive films could be used as food coatings.					
36027986	5	80	theme	bioactive	754:762	arg1	films					764:768	bioactive films	754:768	bioactive films	754:768	Among all the physical properties of bioactive films, only contact angle was reduced.					
36027986	10	81	theme	interaction	1267:1277	arg1	patterns					1279:1286	The interaction patterns	1263:1286	The interaction patterns between starch-casein composite and MPPE bioactives	1263:1338	The interaction patterns between starch-casein composite and MPPE bioactives indicated the development of covalent links.					
36027986	10	82	theme	MPPE	1324:1327	arg1	bioactives					1329:1338	starch-casein composite and MPPE bioactives	1296:1338	starch-casein composite and MPPE bioactives	1296:1338	The interaction patterns between starch-casein composite and MPPE bioactives indicated the development of covalent links.					
36027986	1	83	theme	peel	249:252	arg1	extract					254:260	micellar pomegranate peel extract	228:260	micellar pomegranate peel extract (MPPE)	228:267	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	1	83	theme	peel	249:252	arg1	MPPE					263:266	MPPE	263:266	MPPE	263:266	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	1	84	dep	content	356:362	arg1	respect					327:333	respect	327:333	respect	327:333	Starch-casein composite (solid matter content) based bioactive films incorporated with micellar pomegranate peel extract (MPPE) at different concentrations (10, 25, 50, and 100 wt% with respect to base solid matter content) were developed.					
36027986	10	85	theme	composite	1310:1318	arg1	bioactives					1329:1338	starch-casein composite and MPPE bioactives	1296:1338	starch-casein composite and MPPE bioactives	1296:1338	The interaction patterns between starch-casein composite and MPPE bioactives indicated the development of covalent links.					
36296639	3	0	theme	silica	333:338	arg1	nanocarrier					340:350	a silica nanocarrier	331:350	a silica nanocarrier	331:350	Description is given as to the possibility of employing a modifier, such as a silica nanocarrier, for prolonging the release of amikacin, in addition to using chitosan to improve the properties of the materials, e.g., stability and sorption capacity.					
36296639	4	1	theme	±	620:620	arg1	μg/mg					626:630	25.4 ± 2.2 μg/mg	615:630	25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan)	615:668	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	2	with	μg/mg	679:683	arg1	nanoparticles					724:736	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	3	with	μg/mg	626:630	arg1	nanoparticles					724:736	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	6	4	theme	bacteria	1065:1072	arg1	growth					1055:1060	the growth	1051:1060	the growth of bacteria such as Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa	1051:1171	The matrices were also evaluated for their effectiveness in inhibiting the growth of bacteria such as Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa.					
36296639	7	5	theme	drug	1323:1326	arg1	morphology					1239:1248	morphology	1239:1248	morphology	1239:1248	Concurrent research was conducted on the transdermal absorption, morphology, elemental composition, and thermogravimetric properties of the released drug.					
36296639	7	5	theme	drug	1323:1326	arg1	composition					1261:1271	elemental composition	1251:1271	elemental composition	1251:1271	Concurrent research was conducted on the transdermal absorption, morphology, elemental composition, and thermogravimetric properties of the released drug.					
36296639	7	5	theme	drug	1323:1326	arg1	absorption					1227:1236	the transdermal absorption	1211:1236	the transdermal absorption	1211:1236	Concurrent research was conducted on the transdermal absorption, morphology, elemental composition, and thermogravimetric properties of the released drug.					
36296639	7	5	theme	drug	1323:1326	arg1	properties					1296:1305	thermogravimetric properties	1278:1305	thermogravimetric properties	1278:1305	Concurrent research was conducted on the transdermal absorption, morphology, elemental composition, and thermogravimetric properties of the released drug.					
36296639	4	6	theme	chitosan	660:667	arg1	w/v					653:655	0.05% w/v	647:655	0.05% w/v of chitosan	647:667	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	7	theme	%	651:651	arg1	w/v					653:655	0.05% w/v	647:655	0.05% w/v of chitosan	647:667	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	8	theme	actual	525:530	arg1	composition					532:542	their actual composition	519:542	their actual composition	519:542	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	9	theme	SiO2	701:704	arg1	amikacin					706:713	0.08% w/v SiO2 amikacin	691:713	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	7	10	theme	elemental	1251:1259	arg1	composition					1261:1271	elemental composition	1251:1271	elemental composition	1251:1271	Concurrent research was conducted on the transdermal absorption, morphology, elemental composition, and thermogravimetric properties of the released drug.					
36296639	3	11	theme	amikacin	383:390	arg1	release					372:378	the release	368:378	the release of amikacin	368:390	Description is given as to the possibility of employing a modifier, such as a silica nanocarrier, for prolonging the release of amikacin, in addition to using chitosan to improve the properties of the materials, e.g., stability and sorption capacity.					
36296639	7	12	theme	thermogravimetric	1278:1294	arg1	properties					1296:1305	thermogravimetric properties	1278:1305	thermogravimetric properties	1278:1305	Concurrent research was conducted on the transdermal absorption, morphology, elemental composition, and thermogravimetric properties of the released drug.					
36296639	4	13	theme	w/v	697:699	arg1	amikacin					706:713	0.08% w/v SiO2 amikacin	691:713	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	1	14	theme	porous	78:83	arg1	matrices					85:92	Three porous matrices	72:92	Three porous matrices based on poly(lactic acid)	72:119	Three porous matrices based on poly(lactic acid) are proposed herein for the controlled release of amikacin.					
36296639	1	15	theme	controlled	149:158	arg1	release					160:166	the controlled release	145:166	the controlled release of amikacin	145:178	Three porous matrices based on poly(lactic acid) are proposed herein for the controlled release of amikacin.					
36296639	0	16	theme	Porous	14:19	arg1	Systems					21:27	Antibacterial Porous Systems	0:27	Antibacterial Porous Systems Based on Polylactide	0:48	Antibacterial Porous Systems Based on Polylactide Loaded with Amikacin.					
36296639	5	17	theme	mathematical	917:928	arg1	model					944:948	the mathematical Kosmyer-Pepas model	913:948	the mathematical Kosmyer-Pepas model for all the materials tested	913:977	An in vitro study confirmed extended release of the drug (amikacin, over 60 days), carried out in accordance with the mathematical Kosmyer-Pepas model for all the materials tested.					
36296639	4	18	theme	modified	715:722	arg1	nanoparticles					724:736	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	19	theme	0.05	647:650	arg1	%					651:651	%	651:651	%	651:651	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	0	20	theme	Antibacterial	0:12	arg1	Systems					21:27	Antibacterial Porous Systems	0:27	Antibacterial Porous Systems Based on Polylactide	0:48	Antibacterial Porous Systems Based on Polylactide Loaded with Amikacin.					
36296639	4	21	theme	amikacin	706:713	arg1	nanoparticles					724:736	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	22	with	μg/mg	752:756	arg1	nanoparticles					724:736	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	23	dep	exhibited	559:567	arg1	μg/mg					752:756	96 ± 34 μg/mg	744:756	96 ± 34 μg/mg (matrices without functional additives)	744:796	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	23	dep	exhibited	559:567	arg1	μg/mg					679:683	93 ± 13 μg/mg	671:683	93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles)	671:737	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	23	dep	exhibited	559:567	arg1	μg/mg					626:630	25.4 ± 2.2 μg/mg	615:630	25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan)	615:668	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	24	dep	μg/mg	626:630	arg1	matrices					633:640	matrices	633:640	matrices with 0.05% w/v of chitosan	633:667	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	1	25	theme	amikacin	171:178	arg1	release					160:166	the controlled release	145:166	the controlled release of amikacin	145:178	Three porous matrices based on poly(lactic acid) are proposed herein for the controlled release of amikacin.					
36296639	3	26	theme	sorption	487:494	arg1	capacity					496:503	sorption capacity	487:503	sorption capacity	487:503	Description is given as to the possibility of employing a modifier, such as a silica nanocarrier, for prolonging the release of amikacin, in addition to using chitosan to improve the properties of the materials, e.g., stability and sorption capacity.					
36296639	4	27	theme	functional	776:785	arg1	additives					787:795	functional additives	776:795	functional additives	776:795	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	5	28	theme	drug	851:854	arg1	release					836:842	extended release	827:842	extended release	827:842	An in vitro study confirmed extended release of the drug (amikacin, over 60 days), carried out in accordance with the mathematical Kosmyer-Pepas model for all the materials tested.					
36296639	5	29	dep	amikacin	857:864	arg1	days					875:878	over 60 days	867:878	over 60 days	867:878	An in vitro study confirmed extended release of the drug (amikacin, over 60 days), carried out in accordance with the mathematical Kosmyer-Pepas model for all the materials tested.					
36296639	7	30	theme	released	1314:1321	arg1	drug					1323:1326	the released drug	1310:1326	the released drug	1310:1326	Concurrent research was conducted on the transdermal absorption, morphology, elemental composition, and thermogravimetric properties of the released drug.					
36296639	7	31	theme	transdermal	1215:1225	arg1	absorption					1227:1236	the transdermal absorption	1211:1236	the transdermal absorption	1211:1236	Concurrent research was conducted on the transdermal absorption, morphology, elemental composition, and thermogravimetric properties of the released drug.					
36296639	4	32	theme	±	747:747	arg1	μg/mg					752:756	96 ± 34 μg/mg	744:756	96 ± 34 μg/mg (matrices without functional additives)	744:796	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	33	theme	%	695:695	arg1	amikacin					706:713	0.08% w/v SiO2 amikacin	691:713	0.08% w/v SiO2 amikacin modified nanoparticles	691:736	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	5	34	theme	Kosmyer-Pepas	930:942	arg1	model					944:948	the mathematical Kosmyer-Pepas model	913:948	the mathematical Kosmyer-Pepas model for all the materials tested	913:977	An in vitro study confirmed extended release of the drug (amikacin, over 60 days), carried out in accordance with the mathematical Kosmyer-Pepas model for all the materials tested.					
36296639	4	35	theme	0.08	691:694	arg1	%					695:695	%	695:695	%	695:695	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	1	36	theme	lactic	108:113	arg1	acid					115:118	lactic acid	108:118	lactic acid	108:118	Three porous matrices based on poly(lactic acid) are proposed herein for the controlled release of amikacin.					
36296639	1	36	theme	lactic	108:113	arg1	poly					103:106	poly	103:106	poly(lactic acid)	103:119	Three porous matrices based on poly(lactic acid) are proposed herein for the controlled release of amikacin.					
36296639	7	37	theme	Concurrent	1174:1183	arg1	research					1185:1192	Concurrent research	1174:1192	Concurrent research	1174:1192	Concurrent research was conducted on the transdermal absorption, morphology, elemental composition, and thermogravimetric properties of the released drug.					
36296639	5	38	theme	extended	827:834	arg1	release					836:842	extended release	827:842	extended release	827:842	An in vitro study confirmed extended release of the drug (amikacin, over 60 days), carried out in accordance with the mathematical Kosmyer-Pepas model for all the materials tested.					
36296639	4	39	theme	±	674:674	arg1	μg/mg					679:683	93 ± 13 μg/mg	671:683	93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles)	671:737	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	5	40	dep	in	802:803	arg1	vitro					805:809	vitro	805:809	vitro	805:809	An in vitro study confirmed extended release of the drug (amikacin, over 60 days), carried out in accordance with the mathematical Kosmyer-Pepas model for all the materials tested.					
36296639	5	41	theme	in	802:803	arg1	study					811:815	An in vitro study	799:815	An in vitro study	799:815	An in vitro study confirmed extended release of the drug (amikacin, over 60 days), carried out in accordance with the mathematical Kosmyer-Pepas model for all the materials tested.					
36296639	3	42	dep	stability	473:481	arg1	e.g.					467:470	e.g.	467:470	e.g.	467:470	Description is given as to the possibility of employing a modifier, such as a silica nanocarrier, for prolonging the release of amikacin, in addition to using chitosan to improve the properties of the materials, e.g., stability and sorption capacity.					
36296639	4	43	dep	μg/mg	752:756	arg1	matrices					759:766	matrices	759:766	matrices without functional additives	759:795	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	44	theme	varied	569:574	arg1	efficacy					576:583	varied efficacy	569:583	varied efficacy for drug loading	569:600	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	45	theme	drug	589:592	arg1	loading					594:600	drug loading	589:600	drug loading	589:600	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	4	46	with	matrices	633:640	arg1	w/v					653:655	0.05% w/v	647:655	0.05% w/v of chitosan	647:667	Depending on their actual composition, the materials exhibited varied efficacy for drug loading, as follows: 25.4 ± 2.2 μg/mg (matrices with 0.05% w/v of chitosan), 93 ± 13 μg/mg (with 0.08% w/v SiO2 amikacin modified nanoparticles), and 96 ± 34 μg/mg (matrices without functional additives).					
36296639	3	47	dep	using	408:412	arg1	addition					396:403	addition	396:403	addition	396:403	Description is given as to the possibility of employing a modifier, such as a silica nanocarrier, for prolonging the release of amikacin, in addition to using chitosan to improve the properties of the materials, e.g., stability and sorption capacity.					
36296639	3	48	theme	materials	456:464	arg1	properties					438:447	the properties	434:447	the properties of the materials, e.g., stability and sorption capacity	434:503	Description is given as to the possibility of employing a modifier, such as a silica nanocarrier, for prolonging the release of amikacin, in addition to using chitosan to improve the properties of the materials, e.g., stability and sorption capacity.					
37270140	0	0	theme	soft	111:114	arg1	engineering					123:133	soft tissue engineering	111:133	soft tissue engineering	111:133	Optimizing phenol-modified hyaluronic acid for designing shape-maintaining biofabricated hydrogel scaffolds in soft tissue engineering.					
37270140	4	1	theme	polymer	527:533	arg1	ratios					535:540	the polymer ratios	523:540	the polymer ratios	523:540	By adjusting the polymer ratios, we produced a stable hydrogel with varying responses, from a viscous liquid to a thick gel, and optimized 3D scaffolds that were structurally stable both during and after printing, offering precision and flexibility.					
37270140	1	2	theme	well-printable	166:179	arg1	ink					193:195	a well-printable biomaterial ink	164:195	a well-printable biomaterial ink for 3D printing of shape-maintaining hydrogel scaffolds	164:251	In this study, we developed a well-printable biomaterial ink for 3D printing of shape-maintaining hydrogel scaffolds.					
37270140	5	3	theme	shear-thinning	778:791	arg1	behavior					793:800	shear-thinning behavior	778:800	shear-thinning behavior	778:800	Our ink exhibited shear-thinning behavior and high swelling capacity, as well as ECM-like characteristics and biocompatibility, making it an ideal candidate for soft tissues matrices with storage modulus of around 300 Pa.					
37270140	1	4	theme	biomaterial	181:191	arg1	ink					193:195	a well-printable biomaterial ink	164:195	a well-printable biomaterial ink for 3D printing of shape-maintaining hydrogel scaffolds	164:251	In this study, we developed a well-printable biomaterial ink for 3D printing of shape-maintaining hydrogel scaffolds.					
37270140	5	5	theme	300 Pa	974:979	arg1	modulus					956:962	storage modulus	948:962	storage modulus of around 300 Pa	948:979	Our ink exhibited shear-thinning behavior and high swelling capacity, as well as ECM-like characteristics and biocompatibility, making it an ideal candidate for soft tissues matrices with storage modulus of around 300 Pa.					
37270140	6	6	theme	host	1063:1066	arg1	tissue					1068:1073	host tissue	1063:1073	host tissue	1063:1073	Animal trials and CAM assays confirmed its biocompatibility and integration with host tissue.					
37270140	4	7	theme	liquid	612:617	arg1	viscous					604:610	a viscous	602:610	a viscous liquid to a thick gel	602:632	By adjusting the polymer ratios, we produced a stable hydrogel with varying responses, from a viscous liquid to a thick gel, and optimized 3D scaffolds that were structurally stable both during and after printing, offering precision and flexibility.					
37270140	0	8	theme	tissue	116:121	arg1	engineering					123:133	soft tissue engineering	111:133	soft tissue engineering	111:133	Optimizing phenol-modified hyaluronic acid for designing shape-maintaining biofabricated hydrogel scaffolds in soft tissue engineering.					
37270140	5	9	with	matrices	934:941	arg1	modulus					956:962	storage modulus	948:962	storage modulus of around 300 Pa	948:979	Our ink exhibited shear-thinning behavior and high swelling capacity, as well as ECM-like characteristics and biocompatibility, making it an ideal candidate for soft tissues matrices with storage modulus of around 300 Pa.					
37270140	5	10	theme	ECM-like	841:848	arg1	characteristics					850:864	ECM-like characteristics	841:864	ECM-like characteristics	841:864	Our ink exhibited shear-thinning behavior and high swelling capacity, as well as ECM-like characteristics and biocompatibility, making it an ideal candidate for soft tissues matrices with storage modulus of around 300 Pa.					
37270140	3	11	theme	ink	445:447	arg1	composition					449:459	the ink composition	441:459	the ink composition	441:459	Using the Box-Behnken design, we explored how varying the ink composition affected fiber formation and shape preservation.					
37270140	5	12	theme	swelling	811:818	arg1	capacity					820:827	high swelling capacity	806:827	high swelling capacity	806:827	Our ink exhibited shear-thinning behavior and high swelling capacity, as well as ECM-like characteristics and biocompatibility, making it an ideal candidate for soft tissues matrices with storage modulus of around 300 Pa.					
37270140	5	13	theme	storage	948:954	arg1	modulus					956:962	storage modulus	948:962	storage modulus of around 300 Pa	948:979	Our ink exhibited shear-thinning behavior and high swelling capacity, as well as ECM-like characteristics and biocompatibility, making it an ideal candidate for soft tissues matrices with storage modulus of around 300 Pa.					
37270140	3	14	theme	fiber	470:474	arg1	formation					476:484	fiber formation	470:484	fiber formation	470:484	Using the Box-Behnken design, we explored how varying the ink composition affected fiber formation and shape preservation.					
37270140	0	15	theme	phenol-modified	11:25	arg1	acid					38:41	phenol-modified hyaluronic acid	11:41	phenol-modified hyaluronic acid for designing shape-maintaining biofabricated hydrogel scaffolds in soft tissue engineering	11:133	Optimizing phenol-modified hyaluronic acid for designing shape-maintaining biofabricated hydrogel scaffolds in soft tissue engineering.					
37270140	4	16	with	hydrogel	564:571	arg1	responses					586:594	varying responses	578:594	varying responses	578:594	By adjusting the polymer ratios, we produced a stable hydrogel with varying responses, from a viscous liquid to a thick gel, and optimized 3D scaffolds that were structurally stable both during and after printing, offering precision and flexibility.					
37270140	1	17	theme	3D	201:202	arg1	printing					204:211	3D printing	201:211	3D printing of shape-maintaining hydrogel scaffolds	201:251	In this study, we developed a well-printable biomaterial ink for 3D printing of shape-maintaining hydrogel scaffolds.					
37270140	4	18	theme	varying	578:584	arg1	responses					586:594	varying responses	578:594	varying responses	578:594	By adjusting the polymer ratios, we produced a stable hydrogel with varying responses, from a viscous liquid to a thick gel, and optimized 3D scaffolds that were structurally stable both during and after printing, offering precision and flexibility.					
37270140	3	19	theme	Box-Behnken	397:407	arg1	design					409:414	the Box-Behnken design	393:414	the Box-Behnken design	393:414	Using the Box-Behnken design, we explored how varying the ink composition affected fiber formation and shape preservation.					
37270140	6	20	theme	Animal	982:987	arg1	trials					989:994	Animal trials	982:994	Animal trials	982:994	Animal trials and CAM assays confirmed its biocompatibility and integration with host tissue.					
37270140	5	21	theme	high	806:809	arg1	capacity					820:827	high swelling capacity	806:827	high swelling capacity	806:827	Our ink exhibited shear-thinning behavior and high swelling capacity, as well as ECM-like characteristics and biocompatibility, making it an ideal candidate for soft tissues matrices with storage modulus of around 300 Pa.					
37270140	3	22	theme	shape	490:494	arg1	preservation					496:507	shape preservation	490:507	shape preservation	490:507	Using the Box-Behnken design, we explored how varying the ink composition affected fiber formation and shape preservation.					
37270140	0	23	theme	hyaluronic	27:36	arg1	acid					38:41	phenol-modified hyaluronic acid	11:41	phenol-modified hyaluronic acid for designing shape-maintaining biofabricated hydrogel scaffolds in soft tissue engineering	11:133	Optimizing phenol-modified hyaluronic acid for designing shape-maintaining biofabricated hydrogel scaffolds in soft tissue engineering.					
37270140	2	24	theme	gelatin	329:335	arg1	methacrylate					337:348	gelatin methacrylate	329:348	gelatin methacrylate (GelMA)	329:356	The hydrogel base comprised tyramine-modified hyaluronic acid (HA-Tyr) and gelatin methacrylate (GelMA) and was dually cross-linked.					
37270140	2	24	theme	gelatin	329:335	arg1	GelMA					351:355	GelMA	351:355	GelMA	351:355	The hydrogel base comprised tyramine-modified hyaluronic acid (HA-Tyr) and gelatin methacrylate (GelMA) and was dually cross-linked.					
37270140	1	25	theme	shape-maintaining	216:232	arg1	scaffolds					243:251	shape-maintaining hydrogel scaffolds	216:251	shape-maintaining hydrogel scaffolds	216:251	In this study, we developed a well-printable biomaterial ink for 3D printing of shape-maintaining hydrogel scaffolds.					
37270140	4	26	theme	3D	649:650	arg1	scaffolds					652:660	3D scaffolds	649:660	3D scaffolds that were structurally stable both during and after printing, offering precision and flexibility	649:757	By adjusting the polymer ratios, we produced a stable hydrogel with varying responses, from a viscous liquid to a thick gel, and optimized 3D scaffolds that were structurally stable both during and after printing, offering precision and flexibility.					
37270140	1	27	theme	hydrogel	234:241	arg1	scaffolds					243:251	shape-maintaining hydrogel scaffolds	216:251	shape-maintaining hydrogel scaffolds	216:251	In this study, we developed a well-printable biomaterial ink for 3D printing of shape-maintaining hydrogel scaffolds.					
37270140	4	28	from	viscous	604:610	arg1	responses					586:594	varying responses	578:594	varying responses	578:594	By adjusting the polymer ratios, we produced a stable hydrogel with varying responses, from a viscous liquid to a thick gel, and optimized 3D scaffolds that were structurally stable both during and after printing, offering precision and flexibility.					
37270140	2	29	theme	hydrogel	258:265	arg1	base					267:270	The hydrogel base	254:270	The hydrogel base	254:270	The hydrogel base comprised tyramine-modified hyaluronic acid (HA-Tyr) and gelatin methacrylate (GelMA) and was dually cross-linked.					
37270140	1	30	theme	scaffolds	243:251	arg1	printing					204:211	3D printing	201:211	3D printing of shape-maintaining hydrogel scaffolds	201:251	In this study, we developed a well-printable biomaterial ink for 3D printing of shape-maintaining hydrogel scaffolds.					
37270140	0	31	theme	biofabricated	75:87	arg1	scaffolds					98:106	shape-maintaining biofabricated hydrogel scaffolds	57:106	shape-maintaining biofabricated hydrogel scaffolds	57:106	Optimizing phenol-modified hyaluronic acid for designing shape-maintaining biofabricated hydrogel scaffolds in soft tissue engineering.					
37270140	5	32	theme	soft	921:924	arg1	tissues					926:932	soft tissues	921:932	soft tissues matrices with storage modulus of around 300 Pa	921:979	Our ink exhibited shear-thinning behavior and high swelling capacity, as well as ECM-like characteristics and biocompatibility, making it an ideal candidate for soft tissues matrices with storage modulus of around 300 Pa.					
37270140	0	33	theme	shape-maintaining	57:73	arg1	scaffolds					98:106	shape-maintaining biofabricated hydrogel scaffolds	57:106	shape-maintaining biofabricated hydrogel scaffolds	57:106	Optimizing phenol-modified hyaluronic acid for designing shape-maintaining biofabricated hydrogel scaffolds in soft tissue engineering.					
37270140	4	34	theme	stable	557:562	arg1	hydrogel					564:571	a stable hydrogel	555:571	a stable hydrogel with varying responses, from a viscous liquid to a thick gel,	555:633	By adjusting the polymer ratios, we produced a stable hydrogel with varying responses, from a viscous liquid to a thick gel, and optimized 3D scaffolds that were structurally stable both during and after printing, offering precision and flexibility.					
37270140	6	35	theme	CAM	1000:1002	arg1	assays					1004:1009	CAM assays	1000:1009	CAM assays	1000:1009	Animal trials and CAM assays confirmed its biocompatibility and integration with host tissue.					
37270140	5	36	dep	tissues	926:932	arg1	matrices					934:941	matrices	934:941	matrices	934:941	Our ink exhibited shear-thinning behavior and high swelling capacity, as well as ECM-like characteristics and biocompatibility, making it an ideal candidate for soft tissues matrices with storage modulus of around 300 Pa.					
37270140	2	37	theme	hyaluronic	300:309	arg1	HA-Tyr					317:322	HA-Tyr	317:322	HA-Tyr	317:322	The hydrogel base comprised tyramine-modified hyaluronic acid (HA-Tyr) and gelatin methacrylate (GelMA) and was dually cross-linked.					
37270140	2	37	theme	hyaluronic	300:309	arg1	acid					311:314	tyramine-modified hyaluronic acid	282:314	tyramine-modified hyaluronic acid (HA-Tyr)	282:323	The hydrogel base comprised tyramine-modified hyaluronic acid (HA-Tyr) and gelatin methacrylate (GelMA) and was dually cross-linked.					
37270140	2	38	theme	tyramine-modified	282:298	arg1	HA-Tyr					317:322	HA-Tyr	317:322	HA-Tyr	317:322	The hydrogel base comprised tyramine-modified hyaluronic acid (HA-Tyr) and gelatin methacrylate (GelMA) and was dually cross-linked.					
37270140	2	38	theme	tyramine-modified	282:298	arg1	acid					311:314	tyramine-modified hyaluronic acid	282:314	tyramine-modified hyaluronic acid (HA-Tyr)	282:323	The hydrogel base comprised tyramine-modified hyaluronic acid (HA-Tyr) and gelatin methacrylate (GelMA) and was dually cross-linked.					
37270140	0	39	theme	hydrogel	89:96	arg1	scaffolds					98:106	shape-maintaining biofabricated hydrogel scaffolds	57:106	shape-maintaining biofabricated hydrogel scaffolds	57:106	Optimizing phenol-modified hyaluronic acid for designing shape-maintaining biofabricated hydrogel scaffolds in soft tissue engineering.					
37270140	5	40	theme	ideal	901:905	arg1	candidate					907:915	an ideal candidate	898:915	an ideal candidate for soft tissues matrices with storage modulus of around 300 Pa	898:979	Our ink exhibited shear-thinning behavior and high swelling capacity, as well as ECM-like characteristics and biocompatibility, making it an ideal candidate for soft tissues matrices with storage modulus of around 300 Pa.					
37270140	4	41	theme	thick	624:628	arg1	gel					630:632	a thick gel	622:632	a thick gel	622:632	By adjusting the polymer ratios, we produced a stable hydrogel with varying responses, from a viscous liquid to a thick gel, and optimized 3D scaffolds that were structurally stable both during and after printing, offering precision and flexibility.					
35726791	0	0	theme	apple	93:97	arg1	pectin					99:104	selenylated apple pectin	81:104	selenylated apple pectin	81:104	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	4	1	theme	GSH-Px	688:693	arg1	activity					695:702	GSH-Px activity	688:702	GSH-Px activity	688:702	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	3	2	theme	apple	513:517	arg1	pectin					519:524	selenylated apple pectin	501:524	selenylated apple pectin	501:524	The acute toxicity and protective effect of selenylated apple pectin against UC were investigated by gavage administration in mice.					
35726791	1	3	theme	selenylated	271:281	arg1	pectin					289:294	selenylated apple pectin	271:294	selenylated apple pectin	271:294	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	0	4	theme	selenylated	81:91	arg1	pectin					99:104	selenylated apple pectin	81:104	selenylated apple pectin	81:104	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	0	5	from	characterization	11:26	arg1	sulfate					117:123	dextran sulfate	109:123	dextran sulfate	109:123	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	3	6	theme	pectin	519:524	arg1	toxicity					467:474	acute toxicity	461:474	acute toxicity	461:474	The acute toxicity and protective effect of selenylated apple pectin against UC were investigated by gavage administration in mice.					
35726791	3	6	theme	pectin	519:524	arg1	effect					491:496	protective effect	480:496	protective effect	480:496	The acute toxicity and protective effect of selenylated apple pectin against UC were investigated by gavage administration in mice.					
35726791	1	7	theme	apple	283:287	arg1	pectin					289:294	selenylated apple pectin	271:294	selenylated apple pectin	271:294	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	6	8	theme	molar	909:913	arg1	ratios					915:920	different molar ratios	899:920	different molar ratios	899:920	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	1	9	theme	pectin	289:294	arg1	effect					261:266	protective effect	250:266	protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice	250:366	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	1	9	theme	pectin	289:294	arg1	characterization					213:228	the structural characterization	198:228	the structural characterization	198:228	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	1	9	theme	pectin	289:294	arg1	toxicity					237:244	acute toxicity	231:244	acute toxicity	231:244	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	0	10	theme	pectin	99:104	arg1	assessment					44:53	acute toxicity assessment	29:53	acute toxicity assessment	29:53	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	0	10	theme	pectin	99:104	arg1	effects					70:76	protective effects	59:76	protective effects of selenylated apple pectin on dextran sulfate	59:123	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	0	10	theme	pectin	99:104	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	6	11	theme	different	899:907	arg1	ratios					915:920	different molar ratios	899:920	different molar ratios	899:920	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	6	12	from	ppm	1008:1010	arg1	weights					952:958	different relative molecular weights	923:958	different relative molecular weights	923:958	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	6	12	from	ppm	1008:1010	arg1	ratios					915:920	different molar ratios	899:920	different molar ratios	899:920	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	6	12	from	ppm	1008:1010	arg1	group					994:998	CH2-O group	988:998	CH2-O group	988:998	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	6	12	from	ppm	1008:1010	arg1	peak					982:985	a weakened signal peak	964:985	a weakened signal peak (CH2-O group) at 3-4 ppm	964:1010	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	3	13	theme	gavage	558:563	arg1	administration					565:578	gavage administration	558:578	gavage administration in mice	558:586	The acute toxicity and protective effect of selenylated apple pectin against UC were investigated by gavage administration in mice.					
35726791	4	14	from	contents	669:676	arg1	tissues					729:735	colon tissues	723:735	colon tissues	723:735	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	4	14	from	contents	669:676	arg1	serum					681:685	serum	681:685	serum	681:685	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	4	14	from	contents	669:676	arg1	activity					695:702	GSH-Px activity	688:702	GSH-Px activity	688:702	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	4	14	from	contents	669:676	arg1	content					712:718	MPO content	708:718	MPO content	708:718	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	4	15	theme	state	599:603	arg1	contents					669:676	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents	589:676	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues	589:735	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	7	16	theme	protective	1086:1095	arg1	effect					1097:1102	the protective effect	1082:1102	the protective effect against UC	1082:1113	The selenylated apple pectin showed the protective effect against UC by down-regulating IL-6 and TNF-α contents and up-regulating the IL-10 content in serum, as well as increasing the GSH-Px activity and decreasing the MPO content in colon tissues.					
35726791	9	17	theme	dietary	1461:1467	arg1	evidence					1402:1409	evidence	1402:1409	evidence	1402:1409	These findings provide evidence in support of selenylated apple pectin as a novel dietary selenium supplement for UC protection.					
35726791	9	17	theme	dietary	1461:1467	arg1	selenium					1469:1476	a novel dietary selenium	1453:1476	a novel dietary selenium supplement for UC protection	1453:1505	These findings provide evidence in support of selenylated apple pectin as a novel dietary selenium supplement for UC protection.					
35726791	9	18	theme	selenylated	1425:1435	arg1	pectin					1443:1448	selenylated apple pectin	1425:1448	selenylated apple pectin	1425:1448	These findings provide evidence in support of selenylated apple pectin as a novel dietary selenium supplement for UC protection.					
35726791	9	19	theme	apple	1437:1441	arg1	pectin					1443:1448	selenylated apple pectin	1425:1448	selenylated apple pectin	1425:1448	These findings provide evidence in support of selenylated apple pectin as a novel dietary selenium supplement for UC protection.					
35726791	0	20	theme	dextran	109:115	arg1	sulfate					117:123	dextran sulfate	109:123	dextran sulfate	109:123	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	0	21	from	assessment	44:53	arg1	sulfate					117:123	dextran sulfate	109:123	dextran sulfate	109:123	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	4	22	theme	organ	593:597	arg1	state					599:603	organ state	593:603	organ state	593:603	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	4	23	theme	coefficients	609:620	arg1	contents					669:676	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents	589:676	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues	589:735	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	6	24	theme	molecular	942:950	arg1	weights					952:958	different relative molecular weights	923:958	different relative molecular weights	923:958	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	5	25	theme	apple	797:801	arg1	pectin					803:808	selenylated apple pectin	785:808	selenylated apple pectin	785:808	The results indicated that selenylated apple pectin was non-toxic and contained 244.28 μgselenium per g.					
35726791	6	26	theme	monosaccharide	867:880	arg1	composition					882:892	The monosaccharide composition	863:892	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm	863:1010	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	4	27	theme	colon	723:727	arg1	tissues					729:735	colon tissues	723:735	colon tissues	723:735	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	0	28	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	6	29	theme	relative	933:940	arg1	weights					952:958	different relative molecular weights	923:958	different relative molecular weights	923:958	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	5	30	contain	contained	828:836	arg1	pectin					803:808	selenylated apple pectin	785:808	selenylated apple pectin	785:808	The results indicated that selenylated apple pectin was non-toxic and contained 244.28 μgselenium per g.					
35726791	5	30	contain	contained	828:836	arg2	μgselenium					845:854	244.28 μgselenium	838:854	244.28 μgselenium	838:854	The results indicated that selenylated apple pectin was non-toxic and contained 244.28 μgselenium per g.					
35726791	1	31	theme	structural	202:211	arg1	characterization					213:228	the structural characterization	198:228	the structural characterization	198:228	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	0	32	theme	acute	29:33	arg1	assessment					44:53	acute toxicity assessment	29:53	acute toxicity assessment	29:53	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	6	33	theme	signal	975:980	arg1	group					994:998	CH2-O group	988:998	CH2-O group	988:998	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	6	33	theme	signal	975:980	arg1	peak					982:985	a weakened signal peak	964:985	a weakened signal peak (CH2-O group) at 3-4 ppm	964:1010	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	0	34	theme	ulcerative	140:149	arg1	colitis					151:157	ulcerative colitis	140:157	ulcerative colitis	140:157	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	7	35	from	content	1269:1275	arg1	tissues					1286:1292	colon tissues	1280:1292	colon tissues	1280:1292	The selenylated apple pectin showed the protective effect against UC by down-regulating IL-6 and TNF-α contents and up-regulating the IL-10 content in serum, as well as increasing the GSH-Px activity and decreasing the MPO content in colon tissues.					
35726791	8	36	theme	DSS-induced	1305:1315	arg1	alterations					1317:1327	DSS-induced alterations	1305:1327	DSS-induced alterations	1305:1327	Moreover, DSS-induced alterations were effectively recovered by a high-dose sample.					
35726791	9	37	theme	novel	1455:1459	arg1	evidence					1402:1409	evidence	1402:1409	evidence	1402:1409	These findings provide evidence in support of selenylated apple pectin as a novel dietary selenium supplement for UC protection.					
35726791	9	37	theme	novel	1455:1459	arg1	selenium					1469:1476	a novel dietary selenium	1453:1476	a novel dietary selenium supplement for UC protection	1453:1505	These findings provide evidence in support of selenylated apple pectin as a novel dietary selenium supplement for UC protection.					
35726791	8	38	theme	high-dose	1361:1369	arg1	sample					1371:1376	a high-dose sample	1359:1376	a high-dose sample	1359:1376	Moreover, DSS-induced alterations were effectively recovered by a high-dose sample.					
35726791	6	39	theme	weakened	966:973	arg1	group					994:998	CH2-O group	988:998	CH2-O group	988:998	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	6	39	theme	weakened	966:973	arg1	peak					982:985	a weakened signal peak	964:985	a weakened signal peak (CH2-O group) at 3-4 ppm	964:1010	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	7	40	from	content	1186:1192	arg1	serum					1197:1201	serum	1197:1201	serum	1197:1201	The selenylated apple pectin showed the protective effect against UC by down-regulating IL-6 and TNF-α contents and up-regulating the IL-10 content in serum, as well as increasing the GSH-Px activity and decreasing the MPO content in colon tissues.					
35726791	7	41	theme	GSH-Px	1230:1235	arg1	activity					1237:1244	the GSH-Px activity	1226:1244	the GSH-Px activity	1226:1244	The selenylated apple pectin showed the protective effect against UC by down-regulating IL-6 and TNF-α contents and up-regulating the IL-10 content in serum, as well as increasing the GSH-Px activity and decreasing the MPO content in colon tissues.					
35726791	6	42	with	composition	882:892	arg1	weights					952:958	different relative molecular weights	923:958	different relative molecular weights	923:958	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	6	42	with	composition	882:892	arg1	ratios					915:920	different molar ratios	899:920	different molar ratios	899:920	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	6	42	with	composition	882:892	arg1	group					994:998	CH2-O group	988:998	CH2-O group	988:998	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	6	42	with	composition	882:892	arg1	peak					982:985	a weakened signal peak	964:985	a weakened signal peak (CH2-O group) at 3-4 ppm	964:1010	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	7	43	theme	TNF-α	1143:1147	arg1	contents					1149:1156	TNF-α contents	1143:1156	TNF-α contents	1143:1156	The selenylated apple pectin showed the protective effect against UC by down-regulating IL-6 and TNF-α contents and up-regulating the IL-10 content in serum, as well as increasing the GSH-Px activity and decreasing the MPO content in colon tissues.					
35726791	2	44	theme	ion	415:417	arg1	chromatography					419:432	ion chromatography	415:432	ion chromatography	415:432	Selenylated apple pectin was characterized by ion chromatography, NMR and SEC-RI-MALLS.					
35726791	2	45	theme	apple	381:385	arg1	pectin					387:392	Selenylated apple pectin	369:392	Selenylated apple pectin	369:392	Selenylated apple pectin was characterized by ion chromatography, NMR and SEC-RI-MALLS.					
35726791	1	46	theme	acute	231:235	arg1	toxicity					237:244	acute toxicity	231:244	acute toxicity	231:244	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	0	47	theme	toxicity	35:42	arg1	assessment					44:53	acute toxicity assessment	29:53	acute toxicity assessment	29:53	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	7	48	theme	selenylated	1050:1060	arg1	pectin					1068:1073	The selenylated apple pectin	1046:1073	The selenylated apple pectin	1046:1073	The selenylated apple pectin showed the protective effect against UC by down-regulating IL-6 and TNF-α contents and up-regulating the IL-10 content in serum, as well as increasing the GSH-Px activity and decreasing the MPO content in colon tissues.					
35726791	3	49	theme	acute	461:465	arg1	toxicity					467:474	acute toxicity	461:474	acute toxicity	461:474	The acute toxicity and protective effect of selenylated apple pectin against UC were investigated by gavage administration in mice.					
35726791	1	50	from	colitis	347:353	arg1	mice					363:366	mice	363:366	mice	363:366	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	1	51	from	effect	261:266	arg1	UC					356:357	UC	356:357	UC	356:357	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	1	51	from	effect	261:266	arg1	colitis					347:353	ulcerative colitis	336:353	dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice	299:366	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	0	52	from	effects	70:76	arg1	sulfate					117:123	dextran sulfate	109:123	dextran sulfate	109:123	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	1	53	from	toxicity	237:244	arg1	UC					356:357	UC	356:357	UC	356:357	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	1	53	from	toxicity	237:244	arg1	colitis					347:353	ulcerative colitis	336:353	dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice	299:366	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	7	54	theme	MPO	1265:1267	arg1	content					1269:1275	the MPO content	1261:1275	the MPO content in colon tissues	1261:1292	The selenylated apple pectin showed the protective effect against UC by down-regulating IL-6 and TNF-α contents and up-regulating the IL-10 content in serum, as well as increasing the GSH-Px activity and decreasing the MPO content in colon tissues.					
35726791	5	55	theme	selenylated	785:795	arg1	pectin					803:808	selenylated apple pectin	785:808	selenylated apple pectin	785:808	The results indicated that selenylated apple pectin was non-toxic and contained 244.28 μgselenium per g.					
35726791	1	56	theme	-induced	327:334	arg1	UC					356:357	UC	356:357	UC	356:357	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	1	56	theme	-induced	327:334	arg1	colitis					347:353	ulcerative colitis	336:353	dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice	299:366	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	7	57	theme	apple	1062:1066	arg1	pectin					1068:1073	The selenylated apple pectin	1046:1073	The selenylated apple pectin	1046:1073	The selenylated apple pectin showed the protective effect against UC by down-regulating IL-6 and TNF-α contents and up-regulating the IL-10 content in serum, as well as increasing the GSH-Px activity and decreasing the MPO content in colon tissues.					
35726791	4	58	dep	cytokine	636:643	arg1	TNF-α					662:666	TNF-α	662:666	TNF-α	662:666	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	4	58	dep	cytokine	636:643	arg1	IL-10					652:656	IL-10	652:656	IL-10	652:656	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	4	58	dep	cytokine	636:643	arg1	IL-6					646:649	IL-6	646:649	IL-6	646:649	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	1	59	from	characterization	213:228	arg1	UC					356:357	UC	356:357	UC	356:357	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	1	59	from	characterization	213:228	arg1	colitis					347:353	ulcerative colitis	336:353	dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice	299:366	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	3	60	theme	selenylated	501:511	arg1	pectin					519:524	selenylated apple pectin	501:524	selenylated apple pectin	501:524	The acute toxicity and protective effect of selenylated apple pectin against UC were investigated by gavage administration in mice.					
35726791	3	61	from	administration	565:578	arg1	mice					583:586	mice	583:586	mice	583:586	The acute toxicity and protective effect of selenylated apple pectin against UC were investigated by gavage administration in mice.					
35726791	1	62	theme	ulcerative	336:345	arg1	UC					356:357	UC	356:357	UC	356:357	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	1	62	theme	ulcerative	336:345	arg1	colitis					347:353	ulcerative colitis	336:353	dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice	299:366	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	3	63	theme	protective	480:489	arg1	effect					491:496	protective effect	480:496	protective effect	480:496	The acute toxicity and protective effect of selenylated apple pectin against UC were investigated by gavage administration in mice.					
35726791	6	64	theme	CH2-O	988:992	arg1	group					994:998	CH2-O group	988:998	CH2-O group	988:998	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	6	64	theme	CH2-O	988:992	arg1	peak					982:985	a weakened signal peak	964:985	a weakened signal peak (CH2-O group) at 3-4 ppm	964:1010	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	4	65	theme	MPO	708:710	arg1	content					712:718	MPO content	708:718	MPO content	708:718	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	1	66	theme	protective	250:259	arg1	effect					261:266	protective effect	250:266	protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice	250:366	This study was aimed at investigating the structural characterization, acute toxicity and protective effect of selenylated apple pectin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.					
35726791	9	67	theme	supplement	1478:1487	arg1	evidence					1402:1409	evidence	1402:1409	evidence	1402:1409	These findings provide evidence in support of selenylated apple pectin as a novel dietary selenium supplement for UC protection.					
35726791	9	67	theme	supplement	1478:1487	arg1	selenium					1469:1476	a novel dietary selenium	1453:1476	a novel dietary selenium supplement for UC protection	1453:1505	These findings provide evidence in support of selenylated apple pectin as a novel dietary selenium supplement for UC protection.					
35726791	7	68	theme	colon	1280:1284	arg1	tissues					1286:1292	colon tissues	1280:1292	colon tissues	1280:1292	The selenylated apple pectin showed the protective effect against UC by down-regulating IL-6 and TNF-α contents and up-regulating the IL-10 content in serum, as well as increasing the GSH-Px activity and decreasing the MPO content in colon tissues.					
35726791	3	69	dep	toxicity	467:474	arg1	The					457:459	The	457:459	The	457:459	The acute toxicity and protective effect of selenylated apple pectin against UC were investigated by gavage administration in mice.					
35726791	2	70	theme	Selenylated	369:379	arg1	pectin					387:392	Selenylated apple pectin	369:392	Selenylated apple pectin	369:392	Selenylated apple pectin was characterized by ion chromatography, NMR and SEC-RI-MALLS.					
35726791	9	71	theme	pectin	1443:1448	arg1	support					1414:1420	support	1414:1420	support of selenylated apple pectin	1414:1448	These findings provide evidence in support of selenylated apple pectin as a novel dietary selenium supplement for UC protection.					
35726791	6	72	theme	3-4	1004:1006	arg1	ppm					1008:1010	3-4 ppm	1004:1010	3-4 ppm	1004:1010	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	0	73	theme	protective	59:68	arg1	effects					70:76	protective effects	59:76	protective effects of selenylated apple pectin on dextran sulfate	59:123	Structural characterization, acute toxicity assessment and protective effects of selenylated apple pectin on dextran sulfate sodium-induced ulcerative colitis.					
35726791	6	74	theme	different	923:931	arg1	weights					952:958	different relative molecular weights	923:958	different relative molecular weights	923:958	The monosaccharide composition with different molar ratios, different relative molecular weights and a weakened signal peak (CH2-O group) at 3-4 ppm were observed after selenylation.					
35726791	7	75	theme	IL-10	1180:1184	arg1	content					1186:1192	the IL-10 content	1176:1192	the IL-10 content in serum	1176:1201	The selenylated apple pectin showed the protective effect against UC by down-regulating IL-6 and TNF-α contents and up-regulating the IL-10 content in serum, as well as increasing the GSH-Px activity and decreasing the MPO content in colon tissues.					
35726791	4	76	theme	inflammatory	623:634	arg1	coefficients					609:620	coefficients	609:620	coefficients	609:620	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
35726791	4	76	theme	inflammatory	623:634	arg1	cytokine					636:643	inflammatory cytokine	623:643	inflammatory cytokine (IL-6, IL-10 and TNF-α)	623:667	The organ state and coefficients, inflammatory cytokine (IL-6, IL-10 and TNF-α) contents in serum, GSH-Px activity and MPO content in colon tissues were also evaluated.					
36565041	3	0	theme	such	451:454	arg1	associations					467:478	such beneficial associations	451:478	such beneficial associations	451:478	To establish such beneficial associations, symbiotic microbes need to colonize the plant tissues via intercellular and/or intracellular infection, a process that requires cell wall modifications.					
36565041	3	1	theme	beneficial	456:465	arg1	associations					467:478	such beneficial associations	451:478	such beneficial associations	451:478	To establish such beneficial associations, symbiotic microbes need to colonize the plant tissues via intercellular and/or intracellular infection, a process that requires cell wall modifications.					
36565041	5	2	theme	symbiotic	951:959	arg1	interface					961:969	the symbiotic interface	947:969	the symbiotic interface	947:969	In this viewpoint, based on several new studies I discuss how the PME-PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface, and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.					
36565041	2	3	theme	several	335:341	arg1	microbes					343:350	several microbes	335:350	several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia)	335:435	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	4	4	contain	have	697:700	arg1	changes					669:675	changes	669:675	changes during this process	669:695	Although cell wall composition and changes during this process have interested researchers for years, the functional characterization of the molecular players involved is still limited.					
36565041	4	4	contain	have	697:700	arg1	composition					653:663	cell wall composition	643:663	cell wall composition	643:663	Although cell wall composition and changes during this process have interested researchers for years, the functional characterization of the molecular players involved is still limited.					
36565041	4	4	contain	have	697:700	arg2	researchers					713:723	interested researchers	702:723	interested researchers	702:723	Although cell wall composition and changes during this process have interested researchers for years, the functional characterization of the molecular players involved is still limited.					
36565041	5	5	theme	cell	913:916	arg1	modifications					930:942	cell wall pectin modifications	913:942	cell wall pectin modifications at the symbiotic interface	913:969	In this viewpoint, based on several new studies I discuss how the PME-PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface, and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.					
36565041	2	6	theme	barrier	249:255	arg1	presence					232:239	the presence	228:239	the presence of this barrier	228:255	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	4	7	theme	cell	643:646	arg1	composition					653:663	cell wall composition	643:663	cell wall composition	643:663	Although cell wall composition and changes during this process have interested researchers for years, the functional characterization of the molecular players involved is still limited.					
36565041	0	8	from	interface	38:46	arg1	modifications					7:19	modifications	7:19	modifications	7:19	Pectin modifications at the symbiotic interface.					
36565041	2	9	dep	microbes	343:350	arg1	fungi					374:378	ectomycorrhizal fungi	358:378	ectomycorrhizal fungi (EMF)	358:384	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	2	9	dep	microbes	343:350	arg1	AMF					417:419	AMF	417:419	AMF	417:419	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	2	9	dep	microbes	343:350	arg1	fungi					410:414	arbuscular mycorrhizal fungi	387:414	arbuscular mycorrhizal fungi (AMF)	387:420	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	2	9	dep	microbes	343:350	arg1	EMF					381:383	EMF	381:383	EMF	381:383	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	2	9	dep	microbes	343:350	arg1	rhizobia					427:434	rhizobia	427:434	rhizobia	427:434	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	5	10	from	interface	961:969	arg1	modifications					930:942	cell wall pectin modifications	913:942	cell wall pectin modifications at the symbiotic interface	913:969	In this viewpoint, based on several new studies I discuss how the PME-PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface, and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.					
36565041	5	11	theme	research	994:1001	arg1	directions					1003:1012	further research directions	986:1012	further research directions which can broaden our understanding of how beneficial root symbioses are established	986:1097	In this viewpoint, based on several new studies I discuss how the PME-PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface, and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.					
36565041	3	12	theme	plant	521:525	arg1	tissues					527:533	the plant tissues	517:533	the plant tissues	517:533	To establish such beneficial associations, symbiotic microbes need to colonize the plant tissues via intercellular and/or intracellular infection, a process that requires cell wall modifications.					
36565041	2	13	theme	ectomycorrhizal	358:372	arg1	fungi					374:378	ectomycorrhizal fungi	358:378	ectomycorrhizal fungi (EMF)	358:384	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	2	13	theme	ectomycorrhizal	358:372	arg1	EMF					381:383	EMF	381:383	EMF	381:383	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	1	14	theme	structured	81:90	arg1	wall					97:100	a structured cell wall	79:100	a structured cell wall	79:100	Plant cells are surrounded by a structured cell wall, which not only defines cell shape but also provides a structural barrier for protection against pathogen infection.					
36565041	1	15	theme	structural	157:166	arg1	barrier					168:174	a structural barrier	155:174	a structural barrier for protection against pathogen infection	155:216	Plant cells are surrounded by a structured cell wall, which not only defines cell shape but also provides a structural barrier for protection against pathogen infection.					
36565041	4	16	theme	interested	702:711	arg1	researchers					713:723	interested researchers	702:723	interested researchers	702:723	Although cell wall composition and changes during this process have interested researchers for years, the functional characterization of the molecular players involved is still limited.					
36565041	1	17	theme	cell	92:95	arg1	wall					97:100	a structured cell wall	79:100	a structured cell wall	79:100	Plant cells are surrounded by a structured cell wall, which not only defines cell shape but also provides a structural barrier for protection against pathogen infection.					
36565041	5	18	theme	further	986:992	arg1	directions					1003:1012	further research directions	986:1012	further research directions which can broaden our understanding of how beneficial root symbioses are established	986:1097	In this viewpoint, based on several new studies I discuss how the PME-PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface, and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.					
36565041	2	19	theme	arbuscular	387:396	arg1	AMF					417:419	AMF	417:419	AMF	417:419	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	2	19	theme	arbuscular	387:396	arg1	fungi					410:414	arbuscular mycorrhizal fungi	387:414	arbuscular mycorrhizal fungi (AMF)	387:420	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	2	20	theme	mycorrhizal	398:408	arg1	AMF					417:419	AMF	417:419	AMF	417:419	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	2	20	theme	mycorrhizal	398:408	arg1	fungi					410:414	arbuscular mycorrhizal fungi	387:414	arbuscular mycorrhizal fungi (AMF)	387:420	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	3	21	theme	symbiotic	481:489	arg1	microbes					491:498	symbiotic microbes	481:498	symbiotic microbes	481:498	To establish such beneficial associations, symbiotic microbes need to colonize the plant tissues via intercellular and/or intracellular infection, a process that requires cell wall modifications.					
36565041	3	22	theme	cell	609:612	arg1	modifications					619:631	cell wall modifications	609:631	cell wall modifications	609:631	To establish such beneficial associations, symbiotic microbes need to colonize the plant tissues via intercellular and/or intracellular infection, a process that requires cell wall modifications.					
36565041	5	23	theme	pectin	923:928	arg1	modifications					930:942	cell wall pectin modifications	913:942	cell wall pectin modifications at the symbiotic interface	913:969	In this viewpoint, based on several new studies I discuss how the PME-PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface, and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.					
36565041	3	24	theme	wall	614:617	arg1	modifications					619:631	cell wall modifications	609:631	cell wall modifications	609:631	To establish such beneficial associations, symbiotic microbes need to colonize the plant tissues via intercellular and/or intracellular infection, a process that requires cell wall modifications.					
36565041	4	25	theme	molecular	775:783	arg1	players					785:791	the molecular players	771:791	the molecular players involved	771:800	Although cell wall composition and changes during this process have interested researchers for years, the functional characterization of the molecular players involved is still limited.					
36565041	5	26	theme	several	848:854	arg1	studies					860:866	several new studies	848:866	several new studies	848:866	In this viewpoint, based on several new studies I discuss how the PME-PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface, and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.					
36565041	0	27	theme	symbiotic	28:36	arg1	interface					38:46	the symbiotic interface	24:46	the symbiotic interface	24:46	Pectin modifications at the symbiotic interface.					
36565041	5	28	theme	beneficial	1057:1066	arg1	symbioses					1073:1081	beneficial root symbioses	1057:1081	beneficial root symbioses	1057:1081	In this viewpoint, based on several new studies I discuss how the PME-PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface, and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.					
36565041	3	29	theme	intracellular	560:572	arg1	infection					574:582	intercellular and/or intracellular infection	539:582	intercellular and/or intracellular infection	539:582	To establish such beneficial associations, symbiotic microbes need to colonize the plant tissues via intercellular and/or intracellular infection, a process that requires cell wall modifications.					
36565041	3	29	theme	intracellular	560:572	arg1	process					587:593	a process	585:593	a process that requires cell wall modifications	585:631	To establish such beneficial associations, symbiotic microbes need to colonize the plant tissues via intercellular and/or intracellular infection, a process that requires cell wall modifications.					
36565041	5	30	theme	root	1068:1071	arg1	symbioses					1073:1081	beneficial root symbioses	1057:1081	beneficial root symbioses	1057:1081	In this viewpoint, based on several new studies I discuss how the PME-PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface, and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.					
36565041	3	31	theme	intercellular	539:551	arg1	infection					574:582	intercellular and/or intracellular infection	539:582	intercellular and/or intracellular infection	539:582	To establish such beneficial associations, symbiotic microbes need to colonize the plant tissues via intercellular and/or intracellular infection, a process that requires cell wall modifications.					
36565041	3	31	theme	intercellular	539:551	arg1	process					587:593	a process	585:593	a process that requires cell wall modifications	585:631	To establish such beneficial associations, symbiotic microbes need to colonize the plant tissues via intercellular and/or intracellular infection, a process that requires cell wall modifications.					
36565041	1	32	theme	Plant	49:53	arg1	cells					55:59	Plant cells	49:59	Plant cells	49:59	Plant cells are surrounded by a structured cell wall, which not only defines cell shape but also provides a structural barrier for protection against pathogen infection.					
36565041	4	33	theme	wall	648:651	arg1	composition					653:663	cell wall composition	643:663	cell wall composition	643:663	Although cell wall composition and changes during this process have interested researchers for years, the functional characterization of the molecular players involved is still limited.					
36565041	2	34	theme	symbioses	306:314	arg1	establishment					277:289	the establishment	273:289	the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia)	273:435	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	5	35	theme	wall	918:921	arg1	modifications					930:942	cell wall pectin modifications	913:942	cell wall pectin modifications at the symbiotic interface	913:969	In this viewpoint, based on several new studies I discuss how the PME-PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface, and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.					
36565041	1	36	theme	pathogen	199:206	arg1	infection					208:216	pathogen infection	199:216	pathogen infection	199:216	Plant cells are surrounded by a structured cell wall, which not only defines cell shape but also provides a structural barrier for protection against pathogen infection.					
36565041	4	37	theme	functional	740:749	arg1	characterization					751:766	the functional characterization	736:766	the functional characterization of the molecular players involved	736:800	Although cell wall composition and changes during this process have interested researchers for years, the functional characterization of the molecular players involved is still limited.					
36565041	2	38	theme	mutualistic	294:304	arg1	symbioses					306:314	mutualistic symbioses	294:314	mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia)	294:435	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	5	39	theme	PME-PL/PG	886:894	arg1	pathway					896:902	the PME-PL/PG pathway	882:902	the PME-PL/PG pathway	882:902	In this viewpoint, based on several new studies I discuss how the PME-PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface, and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.					
36565041	4	40	theme	players	785:791	arg1	characterization					751:766	the functional characterization	736:766	the functional characterization of the molecular players involved	736:800	Although cell wall composition and changes during this process have interested researchers for years, the functional characterization of the molecular players involved is still limited.					
36565041	5	41	theme	new	856:858	arg1	studies					860:866	several new studies	848:866	several new studies	848:866	In this viewpoint, based on several new studies I discuss how the PME-PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface, and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.					
36565041	2	42	dep	fungi	374:378	arg1	e.g.					353:356	e.g.	353:356	e.g.	353:356	However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi (EMF), arbuscular mycorrhizal fungi (AMF), and rhizobia).					
36565041	1	43	theme	cell	126:129	arg1	shape					131:135	cell shape	126:135	cell shape	126:135	Plant cells are surrounded by a structured cell wall, which not only defines cell shape but also provides a structural barrier for protection against pathogen infection.					
35040454	5	0	theme	cells	1060:1064	arg1	apoptosis					1029:1037	the apoptosis	1025:1037	the apoptosis of hypertrophic scar cells	1025:1064	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	5	1	from	burden	1083:1088	arg1	model					1151:1155	a xenografted mouse model	1131:1155	a xenografted mouse model without adverse side effects	1131:1184	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	1	2	theme	gene	138:141	arg1	system					152:157	a good gene delivery system	131:157	a good gene delivery system	131:157	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	1	2	theme	gene	138:141	arg1	prospect					183:190	a good application prospect	164:190	a good application prospect for transdermal administration in skin disorder treatment	164:248	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	5	3	dep	burden	1083:1088	arg1	the					1079:1081	the	1079:1081	the	1079:1081	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	1	4	theme	disorder	231:238	arg1	treatment					240:248	skin disorder treatment	226:248	skin disorder treatment	226:248	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	5	5	from	progression	1094:1104	arg1	model					1151:1155	a xenografted mouse model	1131:1155	a xenografted mouse model without adverse side effects	1131:1184	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	1	6	theme	delivery	143:150	arg1	system					152:157	a good gene delivery system	131:157	a good gene delivery system	131:157	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	1	6	theme	delivery	143:150	arg1	prospect					183:190	a good application prospect	164:190	a good application prospect for transdermal administration in skin disorder treatment	164:248	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	3	7	dep	metalloproteinases	595:612	arg1	TIMP-1					617:622	TIMP-1	617:622	TIMP-1	617:622	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	3	7	dep	metalloproteinases	595:612	arg1	1					614:614	1	614:614	1	614:614	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	6	8	theme	PHA-based	1197:1205	arg1	SNA					1207:1209	our PHA-based SNA	1193:1209	our PHA-based SNA	1193:1209	Thus, our PHA-based SNA represents a new transdermal delivery vehicle for efficient combinatorial chemo and gene therapy, which is expected to treat various skin disorders.					
35040454	3	9	theme	drug	499:502	arg1	co-delivery					457:467	the co-delivery	453:467	the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX),	453:522	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	5	10	theme	xenografted	1133:1143	arg1	model					1151:1155	a xenografted mouse model	1131:1155	a xenografted mouse model without adverse side effects	1131:1184	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	6	11	theme	gene	1295:1298	arg1	therapy					1300:1306	gene therapy	1295:1306	gene therapy	1295:1306	Thus, our PHA-based SNA represents a new transdermal delivery vehicle for efficient combinatorial chemo and gene therapy, which is expected to treat various skin disorders.					
35040454	3	12	theme	hypertrophic	646:657	arg1	HS					666:667	HS	666:667	HS	666:667	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	3	12	theme	hypertrophic	646:657	arg1	scars					659:663	hypertrophic scars	646:663	hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation	646:722	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	5	13	theme	mouse	1145:1149	arg1	model					1151:1155	a xenografted mouse model	1131:1155	a xenografted mouse model without adverse side effects	1131:1184	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	4	14	theme	cellular	877:884	arg1	uptake					886:891	the cellular uptake	873:891	the cellular uptake	873:891	Our study showed that PHA-based SNAs simultaneously bearing TIMP-1 ASO and DOX (termed PHAAD) could significantly promote skin penetration, improve the cellular uptake, and effectively down-regulate the TIMP-1 expression and enhance the cytotoxicity of DOX.					
35040454	3	15	theme	scars	659:663	arg1	treatment					633:641	the treatment	629:641	the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation	629:722	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	3	16	theme	typical	474:480	arg1	drug					499:502	a typical chemotherapeutic drug	472:502	a typical chemotherapeutic drug	472:502	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	3	16	theme	typical	474:480	arg1	doxorubicin					505:515	doxorubicin	505:515	doxorubicin (DOX)	505:521	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	3	17	theme	nucleic	400:406	arg1	acid					408:411	a spherical nucleic acid	388:411	a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation	388:722	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	4	18	theme	TIMP-1	928:933	arg1	expression					935:944	the TIMP-1 expression	924:944	the TIMP-1 expression	924:944	Our study showed that PHA-based SNAs simultaneously bearing TIMP-1 ASO and DOX (termed PHAAD) could significantly promote skin penetration, improve the cellular uptake, and effectively down-regulate the TIMP-1 expression and enhance the cytotoxicity of DOX.					
35040454	6	19	theme	skin	1344:1347	arg1	disorders					1349:1357	various skin disorders	1336:1357	various skin disorders	1336:1357	Thus, our PHA-based SNA represents a new transdermal delivery vehicle for efficient combinatorial chemo and gene therapy, which is expected to treat various skin disorders.					
35040454	3	20	theme	chemotherapeutic	482:497	arg1	drug					499:502	a typical chemotherapeutic drug	472:502	a typical chemotherapeutic drug	472:502	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	3	20	theme	chemotherapeutic	482:497	arg1	doxorubicin					505:515	doxorubicin	505:515	doxorubicin (DOX)	505:521	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	2	21	theme	research	360:367	arg1	worthy					342:347	worthy	342:347	worthy of further research	342:367	However, most of the traditional SNA core materials are non-degradable materials, so it is worthy of further research.					
35040454	5	22	theme	hypertrophic	1042:1053	arg1	cells					1060:1064	hypertrophic scar cells	1042:1064	hypertrophic scar cells	1042:1064	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	4	23	theme	TIMP-1	785:790	arg1	ASO					792:794	TIMP-1 ASO	785:794	TIMP-1 ASO	785:794	Our study showed that PHA-based SNAs simultaneously bearing TIMP-1 ASO and DOX (termed PHAAD) could significantly promote skin penetration, improve the cellular uptake, and effectively down-regulate the TIMP-1 expression and enhance the cytotoxicity of DOX.					
35040454	1	24	theme	Spherical	98:106	arg1	SNA					122:124	SNA	122:124	SNA	122:124	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	1	24	theme	Spherical	98:106	arg1	acid					116:119	Spherical nucleic acid	98:119	Spherical nucleic acid (SNA)	98:125	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	6	25	theme	transdermal	1228:1238	arg1	vehicle					1249:1255	a new transdermal delivery vehicle	1222:1255	a new transdermal delivery vehicle	1222:1255	Thus, our PHA-based SNA represents a new transdermal delivery vehicle for efficient combinatorial chemo and gene therapy, which is expected to treat various skin disorders.					
35040454	2	26	theme	further	352:358	arg1	research					360:367	further research	352:367	further research	352:367	However, most of the traditional SNA core materials are non-degradable materials, so it is worthy of further research.					
35040454	0	27	theme	Transdermal	0:10	arg1	delivery					12:19	Transdermal delivery	0:19	Transdermal delivery of poly-hyaluronic acid-based spherical nucleic acids for chemogene therapy.	0:96	Transdermal delivery of poly-hyaluronic acid-based spherical nucleic acids for chemogene therapy.					
35040454	1	28	theme	nucleic	108:114	arg1	SNA					122:124	SNA	122:124	SNA	122:124	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	1	28	theme	nucleic	108:114	arg1	acid					116:119	Spherical nucleic acid	98:119	Spherical nucleic acid (SNA)	98:125	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	3	29	theme	spherical	390:398	arg1	acid					408:411	a spherical nucleic acid	388:411	a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation	388:722	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	0	30	theme	poly-hyaluronic	24:38	arg1	acids					69:73	poly-hyaluronic acid-based spherical nucleic acids	24:73	poly-hyaluronic acid-based spherical nucleic acids	24:73	Transdermal delivery of poly-hyaluronic acid-based spherical nucleic acids for chemogene therapy.					
35040454	6	31	theme	combinatorial	1271:1283	arg1	chemo					1285:1289	efficient combinatorial chemo	1261:1289	efficient combinatorial chemo	1261:1289	Thus, our PHA-based SNA represents a new transdermal delivery vehicle for efficient combinatorial chemo and gene therapy, which is expected to treat various skin disorders.					
35040454	2	32	theme	SNA	284:286	arg1	materials					293:301	the traditional SNA core materials	268:301	the traditional SNA core materials	268:301	However, most of the traditional SNA core materials are non-degradable materials, so it is worthy of further research.					
35040454	1	33	theme	good	166:169	arg1	system					152:157	a good gene delivery system	131:157	a good gene delivery system	131:157	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	1	33	theme	good	166:169	arg1	prospect					183:190	a good application prospect	164:190	a good application prospect for transdermal administration in skin disorder treatment	164:248	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	2	34	theme	core	288:291	arg1	materials					293:301	the traditional SNA core materials	268:301	the traditional SNA core materials	268:301	However, most of the traditional SNA core materials are non-degradable materials, so it is worthy of further research.					
35040454	5	35	theme	hypertrophic	1109:1120	arg1	scars					1122:1126	hypertrophic scars	1109:1126	hypertrophic scars	1109:1126	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	6	36	theme	efficient	1261:1269	arg1	chemo					1285:1289	efficient combinatorial chemo	1261:1289	efficient combinatorial chemo	1261:1289	Thus, our PHA-based SNA represents a new transdermal delivery vehicle for efficient combinatorial chemo and gene therapy, which is expected to treat various skin disorders.					
35040454	3	37	theme	tissue	575:580	arg1	inhibitor					582:590	the tissue inhibitor	571:590	the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation	571:722	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	2	38	theme	traditional	272:282	arg1	materials					293:301	the traditional SNA core materials	268:301	the traditional SNA core materials	268:301	However, most of the traditional SNA core materials are non-degradable materials, so it is worthy of further research.					
35040454	1	39	theme	application	171:181	arg1	system					152:157	a good gene delivery system	131:157	a good gene delivery system	131:157	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	1	39	theme	application	171:181	arg1	prospect					183:190	a good application prospect	164:190	a good application prospect for transdermal administration in skin disorder treatment	164:248	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	0	40	theme	spherical	51:59	arg1	acids					69:73	poly-hyaluronic acid-based spherical nucleic acids	24:73	poly-hyaluronic acid-based spherical nucleic acids	24:73	Transdermal delivery of poly-hyaluronic acid-based spherical nucleic acids for chemogene therapy.					
35040454	5	41	theme	scars	1122:1126	arg1	burden					1083:1088	burden	1083:1088	burden	1083:1088	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	5	41	theme	scars	1122:1126	arg1	progression					1094:1104	progression	1094:1104	progression	1094:1104	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	1	42	contain	has	160:162	arg2	system					152:157	a good gene delivery system	131:157	a good gene delivery system	131:157	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	1	42	contain	has	160:162	arg1	SNA					122:124	SNA	122:124	SNA	122:124	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	1	42	contain	has	160:162	arg2	prospect					183:190	a good application prospect	164:190	a good application prospect for transdermal administration in skin disorder treatment	164:248	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	1	42	contain	has	160:162	arg1	acid					116:119	Spherical nucleic acid	98:119	Spherical nucleic acid (SNA)	98:125	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	0	43	theme	acid-based	40:49	arg1	acids					69:73	poly-hyaluronic acid-based spherical nucleic acids	24:73	poly-hyaluronic acid-based spherical nucleic acids	24:73	Transdermal delivery of poly-hyaluronic acid-based spherical nucleic acids for chemogene therapy.					
35040454	4	44	theme	skin	847:850	arg1	penetration					852:862	skin penetration	847:862	skin penetration	847:862	Our study showed that PHA-based SNAs simultaneously bearing TIMP-1 ASO and DOX (termed PHAAD) could significantly promote skin penetration, improve the cellular uptake, and effectively down-regulate the TIMP-1 expression and enhance the cytotoxicity of DOX.					
35040454	3	45	theme	antisense	531:539	arg1	ASO					558:560	ASO	558:560	ASO	558:560	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	3	45	theme	antisense	531:539	arg1	oligonucleotide					541:555	an antisense oligonucleotide	528:555	an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation	528:722	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	0	46	theme	acids	69:73	arg1	delivery					12:19	Transdermal delivery	0:19	Transdermal delivery of poly-hyaluronic acid-based spherical nucleic acids for chemogene therapy.	0:96	Transdermal delivery of poly-hyaluronic acid-based spherical nucleic acids for chemogene therapy.					
35040454	3	47	theme	abnormal	690:697	arg1	proliferation					710:722	abnormal fibroblast proliferation	690:722	abnormal fibroblast proliferation	690:722	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	6	48	theme	delivery	1240:1247	arg1	vehicle					1249:1255	a new transdermal delivery vehicle	1222:1255	a new transdermal delivery vehicle	1222:1255	Thus, our PHA-based SNA represents a new transdermal delivery vehicle for efficient combinatorial chemo and gene therapy, which is expected to treat various skin disorders.					
35040454	4	49	theme	PHA-based	747:755	arg1	SNAs					757:760	PHA-based SNAs	747:760	PHA-based SNAs simultaneously bearing TIMP-1 ASO and DOX (termed PHAAD)	747:817	Our study showed that PHA-based SNAs simultaneously bearing TIMP-1 ASO and DOX (termed PHAAD) could significantly promote skin penetration, improve the cellular uptake, and effectively down-regulate the TIMP-1 expression and enhance the cytotoxicity of DOX.					
35040454	6	50	theme	new	1224:1226	arg1	vehicle					1249:1255	a new transdermal delivery vehicle	1222:1255	a new transdermal delivery vehicle	1222:1255	Thus, our PHA-based SNA represents a new transdermal delivery vehicle for efficient combinatorial chemo and gene therapy, which is expected to treat various skin disorders.					
35040454	0	51	theme	nucleic	61:67	arg1	acids					69:73	poly-hyaluronic acid-based spherical nucleic acids	24:73	poly-hyaluronic acid-based spherical nucleic acids	24:73	Transdermal delivery of poly-hyaluronic acid-based spherical nucleic acids for chemogene therapy.					
35040454	4	52	theme	termed	805:810	arg1	DOX					800:802	DOX	800:802	DOX (termed PHAAD)	800:817	Our study showed that PHA-based SNAs simultaneously bearing TIMP-1 ASO and DOX (termed PHAAD) could significantly promote skin penetration, improve the cellular uptake, and effectively down-regulate the TIMP-1 expression and enhance the cytotoxicity of DOX.					
35040454	4	52	theme	termed	805:810	arg1	PHAAD					812:816	termed PHAAD	805:816	termed PHAAD	805:816	Our study showed that PHA-based SNAs simultaneously bearing TIMP-1 ASO and DOX (termed PHAAD) could significantly promote skin penetration, improve the cellular uptake, and effectively down-regulate the TIMP-1 expression and enhance the cytotoxicity of DOX.					
35040454	6	53	theme	various	1336:1342	arg1	disorders					1349:1357	various skin disorders	1336:1357	various skin disorders	1336:1357	Thus, our PHA-based SNA represents a new transdermal delivery vehicle for efficient combinatorial chemo and gene therapy, which is expected to treat various skin disorders.					
35040454	5	54	theme	adverse	1165:1171	arg1	effects					1178:1184	adverse side effects	1165:1184	adverse side effects	1165:1184	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	2	55	theme	non-degradable	307:320	arg1	materials					322:330	non-degradable materials	307:330	non-degradable materials	307:330	However, most of the traditional SNA core materials are non-degradable materials, so it is worthy of further research.					
35040454	2	55	theme	non-degradable	307:320	arg1	most					260:263	most	260:263	most	260:263	However, most of the traditional SNA core materials are non-degradable materials, so it is worthy of further research.					
35040454	2	55	theme	non-degradable	307:320	arg1	materials					293:301	the traditional SNA core materials	268:301	the traditional SNA core materials	268:301	However, most of the traditional SNA core materials are non-degradable materials, so it is worthy of further research.					
35040454	1	56	theme	transdermal	196:206	arg1	administration					208:221	transdermal administration	196:221	transdermal administration in skin disorder treatment	196:248	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	1	57	from	administration	208:221	arg1	treatment					240:248	skin disorder treatment	226:248	skin disorder treatment	226:248	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	5	58	theme	PHAAD	993:997	arg1	nanoparticles					999:1011	PHAAD nanoparticles	993:1011	PHAAD nanoparticles	993:1011	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	0	59	theme	chemogene	79:87	arg1	therapy					89:95	chemogene therapy	79:95	chemogene therapy	79:95	Transdermal delivery of poly-hyaluronic acid-based spherical nucleic acids for chemogene therapy.					
35040454	3	60	theme	metalloproteinases	595:612	arg1	inhibitor					582:590	the tissue inhibitor	571:590	the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation	571:722	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	3	61	theme	poly-hyaluronic	422:436	arg1	PHA					444:446	PHA	444:446	PHA	444:446	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	3	61	theme	poly-hyaluronic	422:436	arg1	acid					438:441	poly-hyaluronic acid	422:441	poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX),	422:522	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	3	62	theme	fibroblast	699:708	arg1	proliferation					710:722	abnormal fibroblast proliferation	690:722	abnormal fibroblast proliferation	690:722	Herein, we report a spherical nucleic acid based on poly-hyaluronic acid (PHA) for the co-delivery of a typical chemotherapeutic drug, doxorubicin (DOX), and an antisense oligonucleotide (ASO) against the tissue inhibitor of metalloproteinases 1 (TIMP-1) for the treatment of hypertrophic scars (HS) which are caused by abnormal fibroblast proliferation.					
35040454	5	63	theme	scar	1055:1058	arg1	cells					1060:1064	hypertrophic scar cells	1042:1064	hypertrophic scar cells	1042:1064	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	5	64	theme	side	1173:1176	arg1	effects					1178:1184	adverse side effects	1165:1184	adverse side effects	1165:1184	Moreover, PHAAD nanoparticles facilitated the apoptosis of hypertrophic scar cells, and reduced the burden and progression of hypertrophic scars in a xenografted mouse model without adverse side effects.					
35040454	1	65	theme	good	133:136	arg1	system					152:157	a good gene delivery system	131:157	a good gene delivery system	131:157	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	1	65	theme	good	133:136	arg1	prospect					183:190	a good application prospect	164:190	a good application prospect for transdermal administration in skin disorder treatment	164:248	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35040454	4	66	theme	DOX	978:980	arg1	cytotoxicity					962:973	the cytotoxicity	958:973	the cytotoxicity of DOX	958:980	Our study showed that PHA-based SNAs simultaneously bearing TIMP-1 ASO and DOX (termed PHAAD) could significantly promote skin penetration, improve the cellular uptake, and effectively down-regulate the TIMP-1 expression and enhance the cytotoxicity of DOX.					
35040454	1	67	theme	skin	226:229	arg1	treatment					240:248	skin disorder treatment	226:248	skin disorder treatment	226:248	Spherical nucleic acid (SNA), as a good gene delivery system, has a good application prospect for transdermal administration in skin disorder treatment.					
35765003	2	0	theme	appropriate	405:415	arg1	regeneration					417:428	appropriate regeneration	405:428	appropriate regeneration	405:428	The establishment of a straightforward 3D microenvironment is critical to maintaining cell-to-cell interaction and cellular function, leading to appropriate regeneration.					
35765003	1	1	theme	BACKGROUND	113:122	arg1	Hydrogels					124:132	BACKGROUND Hydrogels	113:132	BACKGROUND Hydrogels based on organic/inorganic composites	113:170	BACKGROUND Hydrogels based on organic/inorganic composites have been at the center of attention for the fabrication of engineered bone constructs.					
35765003	6	2	theme	human	1314:1318	arg1	osteoblasts					1320:1330	human osteoblasts	1314:1330	human osteoblasts leading to osteogenic activity and dynamic growth	1314:1380	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	3	3	theme	human	601:605	arg1	osteoblasts					607:617	human osteoblasts	601:617	human osteoblasts	601:617	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	3	4	dep	in	649:650	arg1	vivo					652:655	vivo	652:655	vivo	652:655	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	5	5	theme	osteogenic	979:988	arg1	capacity					990:997	osteogenic capacity	979:997	osteogenic capacity	979:997	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	7	6	theme	histological	1564:1575	arg1	examination					1577:1587	histological examination	1564:1587	histological examination	1564:1587	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	6	7	theme	pathway	1196:1202	arg1	Reaction					1114:1121	Polymerase Chain Reaction	1097:1121	Polymerase Chain Reaction	1097:1121	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	6	7	theme	pathway	1196:1202	arg1	analysis					1130:1137	PCR (Polymerase Chain Reaction) array analysis	1092:1137	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway	1092:1202	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	5	8	theme	cross-linked	920:931	arg1	microspheres					933:944	Sr2+ cross-linked microspheres	915:944	Sr2+ cross-linked microspheres	915:944	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	7	9	link	cross-linked	1407:1418	arg1	microspheres					1420:1431	Sr2+ cross-linked microspheres	1402:1431	Sr2+ cross-linked microspheres	1402:1431	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	4	10	theme	hydrogels	721:729	arg1	properties					707:716	Physicochemical properties	691:716	RESULTS Physicochemical properties of hydrogels	683:729	RESULTS Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups.					
35765003	1	11	theme	constructs	248:257	arg1	fabrication					217:227	the fabrication	213:227	the fabrication of engineered bone constructs	213:257	BACKGROUND Hydrogels based on organic/inorganic composites have been at the center of attention for the fabrication of engineered bone constructs.					
35765003	6	12	theme	Sr2+	1218:1221	arg1	microspheres					1236:1247	Sr2+ cross-linked microspheres	1218:1247	Sr2+ cross-linked microspheres	1218:1247	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	4	13	dep	RESULTS	683:689	arg1	properties					707:716	Physicochemical properties	691:716	RESULTS Physicochemical properties of hydrogels	683:729	RESULTS Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups.					
35765003	3	14	theme	in	649:650	arg1	conditions					657:666	in vitro and in vivo conditions	636:666	in vitro and in vivo conditions	636:666	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	8	15	theme	dynamic	1670:1676	arg1	growth					1678:1683	dynamic growth	1670:1683	dynamic growth	1670:1683	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel can promote functionality and dynamic growth of osteoblasts.					
35765003	7	16	theme	bone	1522:1525	arg1	formation					1527:1535	bone formation	1522:1535	bone formation	1522:1535	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	2	17	theme	3D	299:300	arg1	microenvironment					302:317	a straightforward 3D microenvironment	281:317	a straightforward 3D microenvironment	281:317	The establishment of a straightforward 3D microenvironment is critical to maintaining cell-to-cell interaction and cellular function, leading to appropriate regeneration.					
35765003	6	18	theme	osteogenic	1343:1352	arg1	activity					1354:1361	osteogenic activity	1343:1361	osteogenic activity	1343:1361	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	6	19	theme	Wingless-related	1151:1166	arg1	site					1180:1183	Wingless-related integration site	1151:1183	Wingless-related integration site	1151:1183	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	6	19	theme	Wingless-related	1151:1166	arg1	pathway					1196:1202	the Wnt (Wingless-related integration site) signaling pathway	1142:1202	the Wnt (Wingless-related integration site) signaling pathway	1142:1202	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	0	20	theme	suitable	83:90	arg1	platform					103:110	suitable osteogenic platform	83:110	suitable osteogenic platform	83:110	Interaction of alginate with nano-hydroxyapatite-collagen using strontium provides suitable osteogenic platform.					
35765003	4	21	theme	reduced	788:794	arg1	swelling					796:803	reduced swelling	788:803	reduced swelling	788:803	RESULTS Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups.					
35765003	6	22	theme	Chain	1108:1112	arg1	Reaction					1114:1121	Polymerase Chain Reaction	1097:1121	Polymerase Chain Reaction	1097:1121	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	6	22	theme	Chain	1108:1112	arg1	analysis					1130:1137	PCR (Polymerase Chain Reaction) array analysis	1092:1137	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway	1092:1202	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	5	23	link	cross-linked	920:931	arg1	microspheres					933:944	Sr2+ cross-linked microspheres	915:944	Sr2+ cross-linked microspheres	915:944	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	7	24	theme	rat	1500:1502	arg1	model					1504:1508	the rat model	1496:1508	the rat model	1496:1508	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	5	25	theme	MG-63	890:894	arg1	osteoblasts					896:906	Human MG-63 osteoblasts	884:906	Human MG-63 osteoblasts	884:906	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	5	26	theme	proteins	1056:1063	arg1	viability					965:973	enhanced viability	956:973	enhanced viability	956:973	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	5	26	theme	proteins	1056:1063	arg1	capacity					990:997	osteogenic capacity	979:997	osteogenic capacity	979:997	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	5	26	theme	proteins	1056:1063	arg1	increase					1035:1042	the increase	1031:1042	the increase of relevant proteins related to bone formation	1031:1089	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	6	27	theme	integration	1168:1178	arg1	site					1180:1183	Wingless-related integration site	1151:1183	Wingless-related integration site	1151:1183	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	6	27	theme	integration	1168:1178	arg1	pathway					1196:1202	the Wnt (Wingless-related integration site) signaling pathway	1142:1202	the Wnt (Wingless-related integration site) signaling pathway	1142:1202	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	0	28	theme	alginate	15:22	arg1	Interaction					0:10	Interaction	0:10	Interaction of alginate with nano-hydroxyapatite-collagen using strontium	0:72	Interaction of alginate with nano-hydroxyapatite-collagen using strontium provides suitable osteogenic platform.					
35765003	7	29	theme	size	1481:1484	arg1	defect					1486:1491	critical size defect	1472:1491	critical size defect in the rat model	1472:1508	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	6	30	link	cross-linked	1223:1234	arg1	microspheres					1236:1247	Sr2+ cross-linked microspheres	1218:1247	Sr2+ cross-linked microspheres	1218:1247	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	5	31	theme	enhanced	956:963	arg1	viability					965:973	enhanced viability	956:973	enhanced viability	956:973	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	8	32	theme	CONCLUSION	1590:1599	arg1	hydrogel					1631:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel	1590:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel	1590:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel can promote functionality and dynamic growth of osteoblasts.					
35765003	8	33	theme	cross-linked	1606:1617	arg1	hydrogel					1631:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel	1590:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel	1590:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel can promote functionality and dynamic growth of osteoblasts.					
35765003	0	34	with	Interaction	0:10	arg1	nano-hydroxyapatite-collagen					29:56	nano-hydroxyapatite-collagen	29:56	nano-hydroxyapatite-collagen using strontium	29:72	Interaction of alginate with nano-hydroxyapatite-collagen using strontium provides suitable osteogenic platform.					
35765003	7	35	from	defect	1486:1491	arg1	model					1504:1508	the rat model	1496:1508	the rat model	1496:1508	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	7	36	theme	rat	1438:1440	arg1	osteoblasts					1442:1452	rat osteoblasts	1438:1452	rat osteoblasts	1438:1452	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	3	37	theme	osteogenic	576:585	arg1	properties					587:596	osteogenic properties	576:596	osteogenic properties of human osteoblasts	576:617	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	7	38	theme	microspheres	1420:1431	arg1	Transplantation					1383:1397	Transplantation	1383:1397	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model	1383:1508	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	7	39	theme	Sr2+	1402:1405	arg1	microspheres					1420:1431	Sr2+ cross-linked microspheres	1402:1431	Sr2+ cross-linked microspheres	1402:1431	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	1	40	theme	engineered	232:241	arg1	constructs					248:257	engineered bone constructs	232:257	engineered bone constructs	232:257	BACKGROUND Hydrogels based on organic/inorganic composites have been at the center of attention for the fabrication of engineered bone constructs.					
35765003	3	41	theme	osteoblasts	607:617	arg1	properties					587:596	osteogenic properties	576:596	osteogenic properties of human osteoblasts	576:617	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	3	42	theme	in	636:637	arg1	conditions					657:666	in vitro and in vivo conditions	636:666	in vitro and in vivo conditions	636:666	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	1	43	theme	attention	199:207	arg1	center					189:194	the center	185:194	the center of attention for the fabrication of engineered bone constructs	185:257	BACKGROUND Hydrogels based on organic/inorganic composites have been at the center of attention for the fabrication of engineered bone constructs.					
35765003	2	44	theme	cellular	375:382	arg1	function					384:391	cellular function	375:391	cellular function	375:391	The establishment of a straightforward 3D microenvironment is critical to maintaining cell-to-cell interaction and cellular function, leading to appropriate regeneration.					
35765003	6	45	theme	PCR	1092:1094	arg1	Reaction					1114:1121	Polymerase Chain Reaction	1097:1121	Polymerase Chain Reaction	1097:1121	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	6	45	theme	PCR	1092:1094	arg1	analysis					1130:1137	PCR (Polymerase Chain Reaction) array analysis	1092:1137	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway	1092:1202	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	6	46	theme	transduction	1289:1300	arg1	pathways					1302:1309	various signaling transduction pathways	1271:1309	various signaling transduction pathways	1271:1309	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	7	47	with	cranium	1459:1465	arg1	defect					1486:1491	critical size defect	1472:1491	critical size defect in the rat model	1472:1508	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	5	48	theme	Sr2+	915:918	arg1	microspheres					933:944	Sr2+ cross-linked microspheres	915:944	Sr2+ cross-linked microspheres	915:944	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	6	49	theme	various	1271:1277	arg1	pathways					1302:1309	various signaling transduction pathways	1271:1309	various signaling transduction pathways	1271:1309	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	4	50	theme	Physicochemical	691:705	arg1	properties					707:716	Physicochemical properties	691:716	RESULTS Physicochemical properties of hydrogels	683:729	RESULTS Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups.					
35765003	1	51	theme	bone	243:246	arg1	constructs					248:257	engineered bone constructs	232:257	engineered bone constructs	232:257	BACKGROUND Hydrogels based on organic/inorganic composites have been at the center of attention for the fabrication of engineered bone constructs.					
35765003	1	52	theme	organic/inorganic	143:159	arg1	composites					161:170	organic/inorganic composites	143:170	organic/inorganic composites	143:170	BACKGROUND Hydrogels based on organic/inorganic composites have been at the center of attention for the fabrication of engineered bone constructs.					
35765003	6	53	theme	dynamic	1367:1373	arg1	growth					1375:1380	dynamic growth	1367:1380	dynamic growth	1367:1380	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	4	54	theme	other	870:874	arg1	groups					876:881	the other groups	866:881	the other groups	866:881	RESULTS Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups.					
35765003	2	55	theme	microenvironment	302:317	arg1	establishment					264:276	The establishment	260:276	The establishment of a straightforward 3D microenvironment	260:317	The establishment of a straightforward 3D microenvironment is critical to maintaining cell-to-cell interaction and cellular function, leading to appropriate regeneration.					
35765003	2	55	theme	microenvironment	302:317	arg1	critical					322:329	critical	322:329	critical	322:329	The establishment of a straightforward 3D microenvironment is critical to maintaining cell-to-cell interaction and cellular function, leading to appropriate regeneration.					
35765003	6	56	theme	signaling	1279:1287	arg1	pathways					1302:1309	various signaling transduction pathways	1271:1309	various signaling transduction pathways	1271:1309	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	8	57	theme	osteoblasts	1688:1698	arg1	functionality					1652:1664	functionality	1652:1664	functionality	1652:1664	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel can promote functionality and dynamic growth of osteoblasts.					
35765003	8	57	theme	osteoblasts	1688:1698	arg1	growth					1678:1683	dynamic growth	1670:1683	dynamic growth	1670:1683	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel can promote functionality and dynamic growth of osteoblasts.					
35765003	4	58	theme	enhanced	806:813	arg1	stability					815:823	enhanced stability	806:823	enhanced stability	806:823	RESULTS Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups.					
35765003	0	59	theme	osteogenic	92:101	arg1	platform					103:110	suitable osteogenic platform	83:110	suitable osteogenic platform	83:110	Interaction of alginate with nano-hydroxyapatite-collagen using strontium provides suitable osteogenic platform.					
35765003	2	60	theme	straightforward	283:297	arg1	microenvironment					302:317	a straightforward 3D microenvironment	281:317	a straightforward 3D microenvironment	281:317	The establishment of a straightforward 3D microenvironment is critical to maintaining cell-to-cell interaction and cellular function, leading to appropriate regeneration.					
35765003	6	61	theme	array	1124:1128	arg1	Reaction					1114:1121	Polymerase Chain Reaction	1097:1121	Polymerase Chain Reaction	1097:1121	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	6	61	theme	array	1124:1128	arg1	analysis					1130:1137	PCR (Polymerase Chain Reaction) array analysis	1092:1137	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway	1092:1202	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	8	62	link	cross-linked	1606:1617	arg1	hydrogel					1631:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel	1590:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel	1590:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel can promote functionality and dynamic growth of osteoblasts.					
35765003	7	63	with	Transplantation	1383:1397	arg1	osteoblasts					1442:1452	rat osteoblasts	1438:1452	rat osteoblasts	1438:1452	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	3	64	dep	in	636:637	arg1	vitro					639:643	vitro	639:643	vitro	639:643	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	5	65	theme	bone	1076:1079	arg1	formation					1081:1089	bone formation	1076:1089	bone formation	1076:1089	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	2	66	theme	cell-to-cell	346:357	arg1	interaction					359:369	cell-to-cell interaction	346:369	cell-to-cell interaction	346:369	The establishment of a straightforward 3D microenvironment is critical to maintaining cell-to-cell interaction and cellular function, leading to appropriate regeneration.					
35765003	6	67	theme	Polymerase	1097:1106	arg1	Reaction					1114:1121	Polymerase Chain Reaction	1097:1121	Polymerase Chain Reaction	1097:1121	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	6	67	theme	Polymerase	1097:1106	arg1	analysis					1130:1137	PCR (Polymerase Chain Reaction) array analysis	1092:1137	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway	1092:1202	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	3	68	theme	Ionic	431:435	arg1	Ca2+					452:455	Ca2+	452:455	Ca2+	452:455	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	3	68	theme	Ionic	431:435	arg1	cross-linkers					437:449	Ionic cross-linkers	431:449	Ionic cross-linkers	431:449	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	3	68	theme	Ionic	431:435	arg1	Ba2+					458:461	Ba2+	458:461	Ba2+	458:461	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	3	68	theme	Ionic	431:435	arg1	Sr2+					468:471	Sr2+	468:471	Sr2+	468:471	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	6	69	theme	cross-linked	1223:1234	arg1	microspheres					1236:1247	Sr2+ cross-linked microspheres	1218:1247	Sr2+ cross-linked microspheres	1218:1247	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	7	70	dep	accelerated	1510:1520	arg1	analyzed					1537:1544	analyzed	1537:1544	accelerated bone formation analyzed with micro-CT and histological examination	1510:1587	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	6	71	theme	signaling	1186:1194	arg1	site					1180:1183	Wingless-related integration site	1151:1183	Wingless-related integration site	1151:1183	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	6	71	theme	signaling	1186:1194	arg1	pathway					1196:1202	the Wnt (Wingless-related integration site) signaling pathway	1142:1202	the Wnt (Wingless-related integration site) signaling pathway	1142:1202	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	3	72	used	used	479:482	arg2	Sr2+					468:471	Sr2+	468:471	Sr2+	468:471	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	3	72	used	used	479:482	arg2	cross-linkers					437:449	Ionic cross-linkers	431:449	Ionic cross-linkers	431:449	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	3	72	used	used	479:482	arg2	Ba2+					458:461	Ba2+	458:461	Ba2+	458:461	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	3	72	used	used	479:482	arg2	Ca2+					452:455	Ca2+	452:455	Ca2+	452:455	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	5	73	theme	Human	884:888	arg1	osteoblasts					896:906	Human MG-63 osteoblasts	884:906	Human MG-63 osteoblasts	884:906	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	5	74	theme	relevant	1047:1054	arg1	proteins					1056:1063	relevant proteins	1047:1063	relevant proteins related to bone formation	1047:1089	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	5	75	theme	related	1065:1071	arg1	proteins					1056:1063	relevant proteins	1047:1063	relevant proteins related to bone formation	1047:1089	Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation.					
35765003	7	76	theme	critical	1472:1479	arg1	defect					1486:1491	critical size defect	1472:1491	critical size defect in the rat model	1472:1508	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	4	77	theme	mechanical	830:839	arg1	strength					841:848	mechanical strength	830:848	mechanical strength	830:848	RESULTS Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups.					
35765003	3	78	theme	Alginate-Nanohydroxyapatite-Collagen	507:542	arg1	microspheres					558:569	Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres	507:569	Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres	507:569	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	6	79	theme	Wnt	1146:1148	arg1	site					1180:1183	Wingless-related integration site	1151:1183	Wingless-related integration site	1151:1183	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	6	79	theme	Wnt	1146:1148	arg1	pathway					1196:1202	the Wnt (Wingless-related integration site) signaling pathway	1142:1202	the Wnt (Wingless-related integration site) signaling pathway	1142:1202	PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth.					
35765003	3	80	theme	microspheres	558:569	arg1	fabrication					492:502	the fabrication	488:502	the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres	488:569	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	8	81	theme	Sr2+	1601:1604	arg1	hydrogel					1631:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel	1590:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel	1590:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel can promote functionality and dynamic growth of osteoblasts.					
35765003	3	82	theme	Alg-nHA-Col	545:555	arg1	microspheres					558:569	Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres	507:569	Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres	507:569	Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days.					
35765003	7	83	theme	cross-linked	1407:1418	arg1	microspheres					1420:1431	Sr2+ cross-linked microspheres	1402:1431	Sr2+ cross-linked microspheres	1402:1431	Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination.					
35765003	4	84	contain	had	784:786	arg2	stability					815:823	enhanced stability	806:823	enhanced stability	806:823	RESULTS Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups.					
35765003	4	84	contain	had	784:786	arg2	strength					841:848	mechanical strength	830:848	mechanical strength	830:848	RESULTS Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups.					
35765003	4	84	contain	had	784:786	arg1	microspheres					748:759	microspheres	748:759	microspheres cross-linked with Sr2+	748:782	RESULTS Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups.					
35765003	4	84	contain	had	784:786	arg2	swelling					796:803	reduced swelling	788:803	reduced swelling	788:803	RESULTS Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups.					
35765003	8	85	theme	Alg-nHA-Col	1619:1629	arg1	hydrogel					1631:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel	1590:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel	1590:1638	CONCLUSION Sr2+ cross-linked Alg-nHA-Col hydrogel can promote functionality and dynamic growth of osteoblasts.					
37080950	5	0	theme	VEGF	918:921	arg1	expression					904:913	the expression	900:913	the expression of VEGF, VEGF-R2 genes in endothelial cells and osteopontin, osteocalcin in osteoblasts cells, respectively	900:1021	The composite biomatrix upregulates the expression of VEGF, VEGF-R2 genes in endothelial cells and osteopontin, osteocalcin in osteoblasts cells, respectively.					
37080950	6	1	theme	oxide	1220:1224	arg1	biomaterial					1239:1249	collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial	1172:1249	collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial	1172:1249	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	4	2	theme	bone	840:843	arg1	environment					851:861	the bone micro environment	836:861	the bone micro environment	836:861	The minimal level of reactive oxygen species and superior cytocompatibility help the as-synthesized biomatrix in achieving capillary migration into the bone micro environment.					
37080950	6	3	theme	tissue	1041:1046	arg1	repair					1048:1053	The in vivo hard tissue repair	1024:1053	The in vivo hard tissue repair experiment conducted in a rat model	1024:1089	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	7	4	theme	therapeutic	1405:1415	arg1	value					1417:1421	its therapeutic value	1401:1421	its therapeutic value	1401:1421	The biomaterial matrix gets biointegrated into the bone tissue and exerts its therapeutic value in bringing a faster osseo repair process.					
37080950	3	5	theme	proangiogenic	626:638	arg1	properties					640:649	proangiogenic properties	626:649	proangiogenic properties	626:649	The addition of lanthanum oxide nanoparticles facilitates good osseointegration coupled with simultaneous activation of proangiogenic properties to act as a bone mimicking material.					
37080950	1	6	theme	proangiogenic	309:321	arg1	biomatrix					182:190	A composite biomatrix	170:190	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles	170:292	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial.					
37080950	1	6	theme	proangiogenic	309:321	arg1	biomaterial					357:367	proangiogenic and osteogenic bone tissue repair biomaterial	309:367	proangiogenic and osteogenic bone tissue repair biomaterial	309:367	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial.					
37080950	5	7	theme	composite	868:876	arg1	biomatrix					878:886	The composite biomatrix	864:886	The composite biomatrix	864:886	The composite biomatrix upregulates the expression of VEGF, VEGF-R2 genes in endothelial cells and osteopontin, osteocalcin in osteoblasts cells, respectively.					
37080950	5	8	from	genes	932:936	arg1	cells					953:957	endothelial cells	941:957	endothelial cells	941:957	The composite biomatrix upregulates the expression of VEGF, VEGF-R2 genes in endothelial cells and osteopontin, osteocalcin in osteoblasts cells, respectively.					
37080950	8	9	with	combination	1541:1551	arg1	biopolymers					1581:1591	protein-polysaccharide biopolymers	1558:1591	protein-polysaccharide biopolymers for bone regeneration	1558:1613	The study shows the feasibility of using rare-earth metal nanoparticles in combination with protein-polysaccharide biopolymers for bone regeneration.					
37080950	6	10	theme	collagen-ƙ-carrageenan-hydroxyapatite-lanthanum	1172:1218	arg1	biomaterial					1239:1249	collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial	1172:1249	collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial	1172:1249	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	4	11	theme	reactive	709:716	arg1	species					725:731	reactive oxygen species	709:731	reactive oxygen species	709:731	The minimal level of reactive oxygen species and superior cytocompatibility help the as-synthesized biomatrix in achieving capillary migration into the bone micro environment.					
37080950	0	12	theme	In	128:129	arg1	Osseointegration					136:151	In Vivo Osseointegration	128:151	In Vivo Osseointegration	128:151	Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite as Angio-Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair.					
37080950	6	13	theme	repair	1048:1053	arg1	experiment					1055:1064	The in vivo hard tissue repair experiment	1024:1064	The in vivo hard tissue repair experiment conducted in a rat model	1024:1089	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	6	14	theme	bone	1121:1124	arg1	defect					1126:1131	the bone defect	1117:1131	the bone defect	1117:1131	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	7	15	theme	biomaterial	1331:1341	arg1	matrix					1343:1348	The biomaterial matrix	1327:1348	The biomaterial matrix	1327:1348	The biomaterial matrix gets biointegrated into the bone tissue and exerts its therapeutic value in bringing a faster osseo repair process.					
37080950	3	16	theme	bone	663:666	arg1	material					678:685	a bone mimicking material	661:685	a bone mimicking material	661:685	The addition of lanthanum oxide nanoparticles facilitates good osseointegration coupled with simultaneous activation of proangiogenic properties to act as a bone mimicking material.					
37080950	1	17	theme	osteogenic	327:336	arg1	biomatrix					182:190	A composite biomatrix	170:190	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles	170:292	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial.					
37080950	1	17	theme	osteogenic	327:336	arg1	biomaterial					357:367	proangiogenic and osteogenic bone tissue repair biomaterial	309:367	proangiogenic and osteogenic bone tissue repair biomaterial	309:367	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial.					
37080950	5	18	theme	osteoblasts	991:1001	arg1	cells					1003:1007	osteoblasts cells	991:1007	osteoblasts cells	991:1007	The composite biomatrix upregulates the expression of VEGF, VEGF-R2 genes in endothelial cells and osteopontin, osteocalcin in osteoblasts cells, respectively.					
37080950	3	19	theme	simultaneous	599:610	arg1	activation					612:621	simultaneous activation	599:621	simultaneous activation of proangiogenic properties to act as a bone mimicking material	599:685	The addition of lanthanum oxide nanoparticles facilitates good osseointegration coupled with simultaneous activation of proangiogenic properties to act as a bone mimicking material.					
37080950	4	20	theme	cytocompatibility	746:762	arg1	level					700:704	The minimal level	688:704	The minimal level of reactive oxygen species and superior cytocompatibility	688:762	The minimal level of reactive oxygen species and superior cytocompatibility help the as-synthesized biomatrix in achieving capillary migration into the bone micro environment.					
37080950	2	21	theme	thermal	479:485	arg1	studies					497:503	thermal stability studies	479:503	thermal stability studies	479:503	The biomatrix shows increased physical and biological stability as observed from proteolytic degradation and thermal stability studies.					
37080950	5	22	theme	endothelial	941:951	arg1	cells					953:957	endothelial cells	941:957	endothelial cells	941:957	The composite biomatrix upregulates the expression of VEGF, VEGF-R2 genes in endothelial cells and osteopontin, osteocalcin in osteoblasts cells, respectively.					
37080950	6	23	theme	individual	1296:1305	arg1	constituents					1307:1318	individual constituents	1296:1318	individual constituents alone	1296:1324	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	0	24	dep	In	128:129	arg1	Vivo					131:134	Vivo	131:134	Vivo	131:134	Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite as Angio-Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair.					
37080950	3	25	theme	oxide	532:536	arg1	nanoparticles					538:550	lanthanum oxide nanoparticles	522:550	lanthanum oxide nanoparticles	522:550	The addition of lanthanum oxide nanoparticles facilitates good osseointegration coupled with simultaneous activation of proangiogenic properties to act as a bone mimicking material.					
37080950	6	26	theme	in	1028:1029	arg1	repair					1048:1053	The in vivo hard tissue repair	1024:1053	The in vivo hard tissue repair experiment conducted in a rat model	1024:1089	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	5	27	dep	VEGF-R2	924:930	arg1	genes					932:936	genes	932:936	genes in endothelial cells	932:957	The composite biomatrix upregulates the expression of VEGF, VEGF-R2 genes in endothelial cells and osteopontin, osteocalcin in osteoblasts cells, respectively.					
37080950	6	28	theme	hard	1036:1039	arg1	repair					1048:1053	The in vivo hard tissue repair	1024:1053	The in vivo hard tissue repair experiment conducted in a rat model	1024:1089	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	4	29	theme	capillary	811:819	arg1	migration					821:829	capillary migration	811:829	capillary migration	811:829	The minimal level of reactive oxygen species and superior cytocompatibility help the as-synthesized biomatrix in achieving capillary migration into the bone micro environment.					
37080950	0	30	theme	Lanthanum	0:8	arg1	Oxide					10:14	Lanthanum Oxide	0:14	Lanthanum Oxide	0:14	Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite as Angio-Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair.					
37080950	1	31	theme	bone	338:341	arg1	repair					350:355	bone tissue repair	338:355	proangiogenic and osteogenic bone tissue repair biomaterial	309:367	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial.					
37080950	8	32	theme	protein-polysaccharide	1558:1579	arg1	biopolymers					1581:1591	protein-polysaccharide biopolymers	1558:1591	protein-polysaccharide biopolymers for bone regeneration	1558:1613	The study shows the feasibility of using rare-earth metal nanoparticles in combination with protein-polysaccharide biopolymers for bone regeneration.					
37080950	2	33	theme	proteolytic	451:461	arg1	degradation					463:473	proteolytic degradation	451:473	proteolytic degradation	451:473	The biomatrix shows increased physical and biological stability as observed from proteolytic degradation and thermal stability studies.					
37080950	0	34	theme	Reinforced	30:39	arg1	Biocomposite					79:90	Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite	30:90	Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite	30:90	Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite as Angio-Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair.					
37080950	1	35	theme	tissue	343:348	arg1	repair					350:355	bone tissue repair	338:355	proangiogenic and osteogenic bone tissue repair biomaterial	309:367	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial.					
37080950	7	36	theme	faster	1437:1442	arg1	process					1457:1463	a faster osseo repair process	1435:1463	a faster osseo repair process	1435:1463	The biomaterial matrix gets biointegrated into the bone tissue and exerts its therapeutic value in bringing a faster osseo repair process.					
37080950	3	37	theme	mimicking	668:676	arg1	material					678:685	a bone mimicking material	661:685	a bone mimicking material	661:685	The addition of lanthanum oxide nanoparticles facilitates good osseointegration coupled with simultaneous activation of proangiogenic properties to act as a bone mimicking material.					
37080950	3	38	theme	good	564:567	arg1	osseointegration					569:584	good osseointegration	564:584	good osseointegration coupled with simultaneous activation of proangiogenic properties to act as a bone mimicking material	564:685	The addition of lanthanum oxide nanoparticles facilitates good osseointegration coupled with simultaneous activation of proangiogenic properties to act as a bone mimicking material.					
37080950	1	39	theme	repair	350:355	arg1	biomatrix					182:190	A composite biomatrix	170:190	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles	170:292	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial.					
37080950	1	39	theme	repair	350:355	arg1	biomaterial					357:367	proangiogenic and osteogenic bone tissue repair biomaterial	309:367	proangiogenic and osteogenic bone tissue repair biomaterial	309:367	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial.					
37080950	0	40	theme	Bone	157:160	arg1	Repair					162:167	Bone Repair	157:167	Bone Repair	157:167	Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite as Angio-Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair.					
37080950	8	41	theme	rare-earth	1507:1516	arg1	nanoparticles					1524:1536	rare-earth metal nanoparticles	1507:1536	rare-earth metal nanoparticles	1507:1536	The study shows the feasibility of using rare-earth metal nanoparticles in combination with protein-polysaccharide biopolymers for bone regeneration.					
37080950	3	42	theme	properties	640:649	arg1	activation					612:621	simultaneous activation	599:621	simultaneous activation of proangiogenic properties to act as a bone mimicking material	599:685	The addition of lanthanum oxide nanoparticles facilitates good osseointegration coupled with simultaneous activation of proangiogenic properties to act as a bone mimicking material.					
37080950	0	43	theme	ƙ-Carrageenan	50:62	arg1	Biocomposite					79:90	Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite	30:90	Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite	30:90	Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite as Angio-Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair.					
37080950	6	44	theme	complete	1097:1104	arg1	healing					1106:1112	complete healing	1097:1112	complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial	1097:1249	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	4	45	theme	as-synthesized	773:786	arg1	biomatrix					788:796	the as-synthesized biomatrix	769:796	the as-synthesized biomatrix	769:796	The minimal level of reactive oxygen species and superior cytocompatibility help the as-synthesized biomatrix in achieving capillary migration into the bone micro environment.					
37080950	6	46	theme	biomaterial	1239:1249	arg1	application					1157:1167	the application	1153:1167	the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial	1153:1249	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	3	47	theme	nanoparticles	538:550	arg1	addition					510:517	The addition	506:517	The addition of lanthanum oxide nanoparticles	506:550	The addition of lanthanum oxide nanoparticles facilitates good osseointegration coupled with simultaneous activation of proangiogenic properties to act as a bone mimicking material.					
37080950	0	48	theme	Collagen	41:48	arg1	Biocomposite					79:90	Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite	30:90	Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite	30:90	Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite as Angio-Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair.					
37080950	4	49	theme	minimal	692:698	arg1	level					700:704	The minimal level	688:704	The minimal level of reactive oxygen species and superior cytocompatibility	688:762	The minimal level of reactive oxygen species and superior cytocompatibility help the as-synthesized biomatrix in achieving capillary migration into the bone micro environment.					
37080950	6	50	with	weeks	1142:1146	arg1	application					1157:1167	the application	1153:1167	the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial	1153:1249	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	1	51	theme	lanthanum	264:272	arg1	nanoparticles					280:292	lanthanum oxide nanoparticles	264:292	lanthanum oxide nanoparticles	264:292	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial.					
37080950	3	52	theme	lanthanum	522:530	arg1	nanoparticles					538:550	lanthanum oxide nanoparticles	522:550	lanthanum oxide nanoparticles	522:550	The addition of lanthanum oxide nanoparticles facilitates good osseointegration coupled with simultaneous activation of proangiogenic properties to act as a bone mimicking material.					
37080950	2	53	theme	biological	413:422	arg1	stability					424:432	increased physical and biological stability	390:432	increased physical and biological stability as observed from proteolytic degradation and thermal stability studies	390:503	The biomatrix shows increased physical and biological stability as observed from proteolytic degradation and thermal stability studies.					
37080950	0	54	theme	Hydroxyapatite	64:77	arg1	Biocomposite					79:90	Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite	30:90	Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite	30:90	Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite as Angio-Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair.					
37080950	6	55	theme	rat	1081:1083	arg1	model					1085:1089	a rat model	1079:1089	a rat model	1079:1089	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	7	56	theme	repair	1450:1455	arg1	process					1457:1463	a faster osseo repair process	1435:1463	a faster osseo repair process	1435:1463	The biomaterial matrix gets biointegrated into the bone tissue and exerts its therapeutic value in bringing a faster osseo repair process.					
37080950	5	57	from	osteocalcin	976:986	arg1	cells					1003:1007	osteoblasts cells	991:1007	osteoblasts cells	991:1007	The composite biomatrix upregulates the expression of VEGF, VEGF-R2 genes in endothelial cells and osteopontin, osteocalcin in osteoblasts cells, respectively.					
37080950	4	58	theme	micro	845:849	arg1	environment					851:861	the bone micro environment	836:861	the bone micro environment	836:861	The minimal level of reactive oxygen species and superior cytocompatibility help the as-synthesized biomatrix in achieving capillary migration into the bone micro environment.					
37080950	7	59	theme	osseo	1444:1448	arg1	process					1457:1463	a faster osseo repair process	1435:1463	a faster osseo repair process	1435:1463	The biomaterial matrix gets biointegrated into the bone tissue and exerts its therapeutic value in bringing a faster osseo repair process.					
37080950	2	60	theme	physical	400:407	arg1	stability					424:432	increased physical and biological stability	390:432	increased physical and biological stability as observed from proteolytic degradation and thermal stability studies	390:503	The biomatrix shows increased physical and biological stability as observed from proteolytic degradation and thermal stability studies.					
37080950	8	61	theme	bone	1597:1600	arg1	regeneration					1602:1613	bone regeneration	1597:1613	bone regeneration	1597:1613	The study shows the feasibility of using rare-earth metal nanoparticles in combination with protein-polysaccharide biopolymers for bone regeneration.					
37080950	1	62	theme	oxide	274:278	arg1	nanoparticles					280:292	lanthanum oxide nanoparticles	264:292	lanthanum oxide nanoparticles	264:292	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial.					
37080950	2	63	theme	increased	390:398	arg1	stability					424:432	increased physical and biological stability	390:432	increased physical and biological stability as observed from proteolytic degradation and thermal stability studies	390:503	The biomatrix shows increased physical and biological stability as observed from proteolytic degradation and thermal stability studies.					
37080950	8	64	theme	metal	1518:1522	arg1	nanoparticles					1524:1536	rare-earth metal nanoparticles	1507:1536	rare-earth metal nanoparticles	1507:1536	The study shows the feasibility of using rare-earth metal nanoparticles in combination with protein-polysaccharide biopolymers for bone regeneration.					
37080950	4	65	theme	oxygen	718:723	arg1	species					725:731	reactive oxygen species	709:731	reactive oxygen species	709:731	The minimal level of reactive oxygen species and superior cytocompatibility help the as-synthesized biomatrix in achieving capillary migration into the bone micro environment.					
37080950	0	66	theme	Angio-Osteogenic	95:110	arg1	Biomaterial					112:122	Angio-Osteogenic Biomaterial	95:122	Angio-Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair	95:167	Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ-Carrageenan Hydroxyapatite Biocomposite as Angio-Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair.					
37080950	2	67	theme	stability	487:495	arg1	studies					497:503	thermal stability studies	479:503	thermal stability studies	479:503	The biomatrix shows increased physical and biological stability as observed from proteolytic degradation and thermal stability studies.					
37080950	6	68	theme	nanoparticle	1226:1237	arg1	biomaterial					1239:1249	collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial	1172:1249	collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial	1172:1249	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	1	69	theme	composite	172:180	arg1	biomatrix					182:190	A composite biomatrix	170:190	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles	170:292	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial.					
37080950	1	69	theme	composite	172:180	arg1	biomaterial					357:367	proangiogenic and osteogenic bone tissue repair biomaterial	309:367	proangiogenic and osteogenic bone tissue repair biomaterial	309:367	A composite biomatrix fabricated with collagen, ƙ-carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial.					
37080950	4	70	theme	superior	737:744	arg1	cytocompatibility					746:762	superior cytocompatibility	737:762	superior cytocompatibility	737:762	The minimal level of reactive oxygen species and superior cytocompatibility help the as-synthesized biomatrix in achieving capillary migration into the bone micro environment.					
37080950	6	71	theme	defect	1126:1131	arg1	healing					1106:1112	complete healing	1097:1112	complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial	1097:1249	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	7	72	theme	bone	1378:1381	arg1	tissue					1383:1388	the bone tissue	1374:1388	the bone tissue	1374:1388	The biomaterial matrix gets biointegrated into the bone tissue and exerts its therapeutic value in bringing a faster osseo repair process.					
37080950	6	73	dep	in	1028:1029	arg1	vivo					1031:1034	vivo	1031:1034	vivo	1031:1034	The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen-ƙ-carrageenan-hydroxyapatite-lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone.					
37080950	4	74	theme	species	725:731	arg1	level					700:704	The minimal level	688:704	The minimal level of reactive oxygen species and superior cytocompatibility	688:762	The minimal level of reactive oxygen species and superior cytocompatibility help the as-synthesized biomatrix in achieving capillary migration into the bone micro environment.					
36610581	0	0	theme	selected	74:81	arg1	fraction					90:97	selected lignin fraction	74:97	selected lignin fraction	74:97	Improvement of UV stability of thermoplastic starch matrix by addition of selected lignin fraction - Photooxidative degradation.					
36610581	9	1	theme	UV-C	1211:1214	arg1	light					1216:1220	UV-C light	1211:1220	UV-C light	1211:1220	Mechanical properties were explored before and after exposure to UV-C light.					
36610581	4	2	from	EtOAc	639:643	arg1	soluble					628:634	soluble	628:634	soluble	628:634	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	4	3	theme	KL	565:566	arg1	fraction					569:576	KL, fraction	565:576	fraction	569:576	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	4	3	theme	KL	565:566	arg1	lignin					544:549	The lignin	540:549	The lignin employed	540:558	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	5	4	theme	Ultraviolet-C	703:715	arg1	UV-C					724:727	UV-C	724:727	UV-C	724:727	The samples were exposed to accelerated aging with Ultraviolet-C light (UV-C) for 432 h. Structural changes were measured by FTIR (Fourier-Transform Infrared) spectra.					
36610581	5	4	theme	Ultraviolet-C	703:715	arg1	light					717:721	Ultraviolet-C light	703:721	Ultraviolet-C light (UV-C)	703:728	The samples were exposed to accelerated aging with Ultraviolet-C light (UV-C) for 432 h. Structural changes were measured by FTIR (Fourier-Transform Infrared) spectra.					
36610581	11	5	theme	TPS	1563:1565	arg1	matrix					1567:1572	TPS matrix	1563:1572	TPS matrix	1563:1572	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	10	6	theme	group	1366:1370	arg1	content					1372:1378	higher phenolic hydroxyl group content	1341:1378	higher phenolic hydroxyl group content	1341:1378	It was observed that the least photodegraded films were those resulting from the addition of the lignin fraction with higher phenolic hydroxyl group content.					
36610581	3	7	from	additive	492:499	arg1	matrix					508:513	TPS matrix	504:513	TPS matrix by casting and pressing	504:537	Films were prepared by mixing lignin fractions as additive in TPS matrix by casting and pressing.					
36610581	2	8	theme	urograndis	350:359	arg1	wood					361:364	Eucalyptus urograndis wood	339:364	Eucalyptus urograndis wood	339:364	KL from Eucalyptus urograndis wood was refined by a sequential fractionation process in ethyl acetate (EtOAc).					
36610581	0	9	theme	fraction	90:97	arg1	addition					62:69	addition	62:69	addition of selected lignin fraction - Photooxidative degradation	62:126	Improvement of UV stability of thermoplastic starch matrix by addition of selected lignin fraction - Photooxidative degradation.					
36610581	11	10	theme	stabilizing	1507:1517	arg1	blocker					1543:1549	an UV-C light blocker	1529:1549	an UV-C light blocker additive on TPS matrix	1529:1572	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	10	theme	stabilizing	1507:1517	arg1	effect					1519:1524	effect	1519:1524	stabilizing effect	1507:1524	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	10	theme	stabilizing	1507:1517	arg1	photoprotection					1487:1501	photoprotection	1487:1501	photoprotection	1487:1501	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	10	11	theme	hydroxyl	1357:1364	arg1	content					1372:1378	higher phenolic hydroxyl group content	1341:1378	higher phenolic hydroxyl group content	1341:1378	It was observed that the least photodegraded films were those resulting from the addition of the lignin fraction with higher phenolic hydroxyl group content.					
36610581	4	12	from	soluble	628:634	arg1	EtOAc					639:643	EtOAc	639:643	EtOAc (SOL)	639:649	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	4	12	from	soluble	628:634	arg1	SOL					646:648	SOL	646:648	SOL	646:648	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	0	13	theme	lignin	83:88	arg1	fraction					90:97	selected lignin fraction	74:97	selected lignin fraction	74:97	Improvement of UV stability of thermoplastic starch matrix by addition of selected lignin fraction - Photooxidative degradation.					
36610581	10	14	theme	phenolic	1348:1355	arg1	content					1372:1378	higher phenolic hydroxyl group content	1341:1378	higher phenolic hydroxyl group content	1341:1378	It was observed that the least photodegraded films were those resulting from the addition of the lignin fraction with higher phenolic hydroxyl group content.					
36610581	0	15	theme	Photooxidative	101:114	arg1	degradation					116:126	Photooxidative degradation	101:126	Photooxidative degradation	101:126	Improvement of UV stability of thermoplastic starch matrix by addition of selected lignin fraction - Photooxidative degradation.					
36610581	6	16	theme	Thermal	820:826	arg1	properties					828:837	Thermal properties	820:837	Thermal properties	820:837	Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry).					
36610581	6	16	theme	Thermal	820:826	arg1	temperature					883:893	melting enthalpy, glass transition temperature	848:893	temperature	883:893	Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry).					
36610581	6	16	theme	Thermal	820:826	arg1	decomposition					907:919	thermal decomposition	899:919	thermal decomposition	899:919	Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry).					
36610581	2	17	theme	ethyl	419:423	arg1	EtOAc					434:438	EtOAc	434:438	EtOAc	434:438	KL from Eucalyptus urograndis wood was refined by a sequential fractionation process in ethyl acetate (EtOAc).					
36610581	2	17	theme	ethyl	419:423	arg1	acetate					425:431	ethyl acetate	419:431	ethyl acetate (EtOAc)	419:439	KL from Eucalyptus urograndis wood was refined by a sequential fractionation process in ethyl acetate (EtOAc).					
36610581	6	18	theme	Scanning	958:965	arg1	Calorimetry					967:977	Differential Scanning Calorimetry	945:977	Differential Scanning Calorimetry	945:977	Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry).					
36610581	6	18	theme	Scanning	958:965	arg1	DSC					940:942	DSC	940:942	DSC (Differential Scanning Calorimetry)	940:978	Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry).					
36610581	5	19	theme	Infrared	801:808	arg1	spectra					811:817	FTIR (Fourier-Transform Infrared) spectra	777:817	FTIR (Fourier-Transform Infrared) spectra	777:817	The samples were exposed to accelerated aging with Ultraviolet-C light (UV-C) for 432 h. Structural changes were measured by FTIR (Fourier-Transform Infrared) spectra.					
36610581	1	20	theme	thermoplastic	169:181	arg1	matrix					196:201	thermoplastic starch (TPS) matrix	169:201	thermoplastic starch (TPS) matrix	169:201	This paper examines the additivation of thermoplastic starch (TPS) matrix by selected fractions of kraft lignin (KL) and correlates its structure-performance when exposed to photooxidative degradation.					
36610581	7	21	theme	Scanning	1052:1059	arg1	Microscopy					1070:1079	Scanning Electron Microscopy	1052:1079	Scanning Electron Microscopy	1052:1079	Morphology of the films was obtained by SEM (Scanning Electron Microscopy).					
36610581	7	21	theme	Scanning	1052:1059	arg1	SEM					1047:1049	SEM	1047:1049	SEM (Scanning Electron Microscopy)	1047:1080	Morphology of the films was obtained by SEM (Scanning Electron Microscopy).					
36610581	7	22	theme	Electron	1061:1068	arg1	Microscopy					1070:1079	Scanning Electron Microscopy	1052:1079	Scanning Electron Microscopy	1052:1079	Morphology of the films was obtained by SEM (Scanning Electron Microscopy).					
36610581	7	22	theme	Electron	1061:1068	arg1	SEM					1047:1049	SEM	1047:1049	SEM (Scanning Electron Microscopy)	1047:1080	Morphology of the films was obtained by SEM (Scanning Electron Microscopy).					
36610581	2	23	from	wood	361:364	arg1	KL					331:332	KL	331:332	KL from Eucalyptus urograndis wood	331:364	KL from Eucalyptus urograndis wood was refined by a sequential fractionation process in ethyl acetate (EtOAc).					
36610581	1	24	theme	starch	183:188	arg1	matrix					196:201	thermoplastic starch (TPS) matrix	169:201	thermoplastic starch (TPS) matrix	169:201	This paper examines the additivation of thermoplastic starch (TPS) matrix by selected fractions of kraft lignin (KL) and correlates its structure-performance when exposed to photooxidative degradation.					
36610581	8	25	theme	wettability	1103:1113	arg1	property					1091:1098	Surface property	1083:1098	Surface property of wettability	1083:1113	Surface property of wettability was measured by contact angle.					
36610581	10	26	theme	higher	1341:1346	arg1	content					1372:1378	higher phenolic hydroxyl group content	1341:1378	higher phenolic hydroxyl group content	1341:1378	It was observed that the least photodegraded films were those resulting from the addition of the lignin fraction with higher phenolic hydroxyl group content.					
36610581	10	27	with	addition	1304:1311	arg1	content					1372:1378	higher phenolic hydroxyl group content	1341:1378	higher phenolic hydroxyl group content	1341:1378	It was observed that the least photodegraded films were those resulting from the addition of the lignin fraction with higher phenolic hydroxyl group content.					
36610581	4	28	theme	KL	625:626	arg1	fraction					613:620	fraction	613:620	fraction of KL soluble in EtOAc (SOL)	613:649	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	4	28	theme	KL	625:626	arg1	fraction					569:576	KL, fraction	565:576	fraction	569:576	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	4	28	theme	KL	625:626	arg1	lignin					544:549	The lignin	540:549	The lignin employed	540:558	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	5	29	theme	432 h.	734:739	arg1	changes					752:758	432 h. Structural changes	734:758	432 h. Structural changes	734:758	The samples were exposed to accelerated aging with Ultraviolet-C light (UV-C) for 432 h. Structural changes were measured by FTIR (Fourier-Transform Infrared) spectra.					
36610581	8	30	theme	Surface	1083:1089	arg1	property					1091:1098	Surface property	1083:1098	Surface property of wettability	1083:1113	Surface property of wettability was measured by contact angle.					
36610581	10	31	theme	fraction	1327:1334	arg1	addition					1304:1311	the addition	1300:1311	the addition of the lignin fraction with higher phenolic hydroxyl group content	1300:1378	It was observed that the least photodegraded films were those resulting from the addition of the lignin fraction with higher phenolic hydroxyl group content.					
36610581	5	32	theme	Fourier-Transform	783:799	arg1	spectra					811:817	FTIR (Fourier-Transform Infrared) spectra	777:817	FTIR (Fourier-Transform Infrared) spectra	777:817	The samples were exposed to accelerated aging with Ultraviolet-C light (UV-C) for 432 h. Structural changes were measured by FTIR (Fourier-Transform Infrared) spectra.					
36610581	1	33	theme	TPS	191:193	arg1	matrix					196:201	thermoplastic starch (TPS) matrix	169:201	thermoplastic starch (TPS) matrix	169:201	This paper examines the additivation of thermoplastic starch (TPS) matrix by selected fractions of kraft lignin (KL) and correlates its structure-performance when exposed to photooxidative degradation.					
36610581	0	34	theme	stability	18:26	arg1	Improvement					0:10	Improvement	0:10	Improvement of UV stability of thermoplastic starch matrix by addition of selected lignin fraction - Photooxidative degradation.	0:127	Improvement of UV stability of thermoplastic starch matrix by addition of selected lignin fraction - Photooxidative degradation.					
36610581	6	35	theme	thermal	899:905	arg1	decomposition					907:919	thermal decomposition	899:919	thermal decomposition	899:919	Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry).					
36610581	3	36	from	matrix	508:513	arg1	additive					492:499	additive	492:499	additive	492:499	Films were prepared by mixing lignin fractions as additive in TPS matrix by casting and pressing.					
36610581	8	37	theme	contact	1131:1137	arg1	angle					1139:1143	contact angle	1131:1143	contact angle	1131:1143	Surface property of wettability was measured by contact angle.					
36610581	11	38	theme	structural	1394:1403	arg1	observations					1423:1434	structural and morphological observations	1394:1434	structural and morphological observations	1394:1434	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	10	39	theme	lignin	1320:1325	arg1	fraction					1327:1334	the lignin fraction	1316:1334	the lignin fraction	1316:1334	It was observed that the least photodegraded films were those resulting from the addition of the lignin fraction with higher phenolic hydroxyl group content.					
36610581	0	40	theme	UV	15:16	arg1	stability					18:26	UV stability	15:26	UV stability of thermoplastic starch matrix	15:57	Improvement of UV stability of thermoplastic starch matrix by addition of selected lignin fraction - Photooxidative degradation.					
36610581	0	41	theme	degradation	116:126	arg1	addition					62:69	addition	62:69	addition of selected lignin fraction - Photooxidative degradation	62:126	Improvement of UV stability of thermoplastic starch matrix by addition of selected lignin fraction - Photooxidative degradation.					
36610581	9	42	theme	Mechanical	1146:1155	arg1	properties					1157:1166	Mechanical properties	1146:1166	Mechanical properties	1146:1166	Mechanical properties were explored before and after exposure to UV-C light.					
36610581	1	43	theme	matrix	196:201	arg1	additivation					153:164	the additivation	149:164	the additivation of thermoplastic starch (TPS) matrix	149:201	This paper examines the additivation of thermoplastic starch (TPS) matrix by selected fractions of kraft lignin (KL) and correlates its structure-performance when exposed to photooxidative degradation.					
36610581	0	44	theme	thermoplastic	31:43	arg1	matrix					52:57	thermoplastic starch matrix	31:57	thermoplastic starch matrix	31:57	Improvement of UV stability of thermoplastic starch matrix by addition of selected lignin fraction - Photooxidative degradation.					
36610581	4	45	from	EtOAc	597:601	arg1	insoluble					584:592	insoluble	584:592	insoluble	584:592	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	2	46	theme	fractionation	394:406	arg1	process					408:414	a sequential fractionation process	381:414	a sequential fractionation process in ethyl acetate (EtOAc)	381:439	KL from Eucalyptus urograndis wood was refined by a sequential fractionation process in ethyl acetate (EtOAc).					
36610581	6	47	theme	transition	872:881	arg1	temperature					883:893	melting enthalpy, glass transition temperature	848:893	temperature	883:893	Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry).					
36610581	11	48	theme	highest	1479:1485	arg1	photoprotection					1487:1501	photoprotection	1487:1501	photoprotection	1487:1501	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	48	theme	highest	1479:1485	arg1	effect					1519:1524	effect	1519:1524	stabilizing effect	1507:1524	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	48	theme	highest	1479:1485	arg1	blocker					1543:1549	an UV-C light blocker	1529:1549	an UV-C light blocker additive on TPS matrix	1529:1572	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	5	49	theme	Structural	741:750	arg1	changes					752:758	432 h. Structural changes	734:758	432 h. Structural changes	734:758	The samples were exposed to accelerated aging with Ultraviolet-C light (UV-C) for 432 h. Structural changes were measured by FTIR (Fourier-Transform Infrared) spectra.					
36610581	1	50	theme	selected	206:213	arg1	fractions					215:223	selected fractions	206:223	selected fractions of kraft lignin (KL)	206:244	This paper examines the additivation of thermoplastic starch (TPS) matrix by selected fractions of kraft lignin (KL) and correlates its structure-performance when exposed to photooxidative degradation.					
36610581	0	51	theme	matrix	52:57	arg1	stability					18:26	UV stability	15:26	UV stability of thermoplastic starch matrix	15:57	Improvement of UV stability of thermoplastic starch matrix by addition of selected lignin fraction - Photooxidative degradation.					
36610581	11	52	dep	photoprotection	1487:1501	arg1	the					1475:1477	the	1475:1477	the	1475:1477	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	53	theme	UV-C	1532:1535	arg1	blocker					1543:1549	an UV-C light blocker	1529:1549	an UV-C light blocker additive on TPS matrix	1529:1572	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	53	theme	UV-C	1532:1535	arg1	effect					1519:1524	effect	1519:1524	stabilizing effect	1507:1524	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	53	theme	UV-C	1532:1535	arg1	photoprotection					1487:1501	photoprotection	1487:1501	photoprotection	1487:1501	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	10	54	theme	photodegraded	1254:1266	arg1	those					1279:1283	those	1279:1283	those	1279:1283	It was observed that the least photodegraded films were those resulting from the addition of the lignin fraction with higher phenolic hydroxyl group content.					
36610581	10	54	theme	photodegraded	1254:1266	arg1	films					1268:1272	the least photodegraded films	1244:1272	the least photodegraded films	1244:1272	It was observed that the least photodegraded films were those resulting from the addition of the lignin fraction with higher phenolic hydroxyl group content.					
36610581	2	55	theme	Eucalyptus	339:348	arg1	wood					361:364	Eucalyptus urograndis wood	339:364	Eucalyptus urograndis wood	339:364	KL from Eucalyptus urograndis wood was refined by a sequential fractionation process in ethyl acetate (EtOAc).					
36610581	0	56	theme	starch	45:50	arg1	matrix					52:57	thermoplastic starch matrix	31:57	thermoplastic starch matrix	31:57	Improvement of UV stability of thermoplastic starch matrix by addition of selected lignin fraction - Photooxidative degradation.					
36610581	1	57	theme	photooxidative	303:316	arg1	degradation					318:328	photooxidative degradation	303:328	photooxidative degradation	303:328	This paper examines the additivation of thermoplastic starch (TPS) matrix by selected fractions of kraft lignin (KL) and correlates its structure-performance when exposed to photooxidative degradation.					
36610581	7	58	theme	films	1025:1029	arg1	Morphology					1007:1016	Morphology	1007:1016	Morphology of the films	1007:1029	Morphology of the films was obtained by SEM (Scanning Electron Microscopy).					
36610581	10	59	theme	least	1248:1252	arg1	those					1279:1283	those	1279:1283	those	1279:1283	It was observed that the least photodegraded films were those resulting from the addition of the lignin fraction with higher phenolic hydroxyl group content.					
36610581	10	59	theme	least	1248:1252	arg1	films					1268:1272	the least photodegraded films	1244:1272	the least photodegraded films	1244:1272	It was observed that the least photodegraded films were those resulting from the addition of the lignin fraction with higher phenolic hydroxyl group content.					
36610581	6	60	theme	enthalpy	856:863	arg1	temperature					883:893	melting enthalpy, glass transition temperature	848:893	temperature	883:893	Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry).					
36610581	6	61	dep	temperature	883:893	arg1	glass					866:870	melting enthalpy, glass transition temperature	848:893	glass	866:870	Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry).					
36610581	4	62	theme	KL	581:582	arg1	fraction					613:620	fraction	613:620	fraction of KL soluble in EtOAc (SOL)	613:649	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	4	62	theme	KL	581:582	arg1	fraction					569:576	KL, fraction	565:576	fraction	569:576	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	4	62	theme	KL	581:582	arg1	lignin					544:549	The lignin	540:549	The lignin employed	540:558	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	11	63	theme	morphological	1409:1421	arg1	observations					1423:1434	structural and morphological observations	1394:1434	structural and morphological observations	1394:1434	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	64	theme	additive	1551:1558	arg1	blocker					1543:1549	an UV-C light blocker	1529:1549	an UV-C light blocker additive on TPS matrix	1529:1572	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	64	theme	additive	1551:1558	arg1	effect					1519:1524	effect	1519:1524	stabilizing effect	1507:1524	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	64	theme	additive	1551:1558	arg1	photoprotection					1487:1501	photoprotection	1487:1501	photoprotection	1487:1501	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	3	65	theme	lignin	472:477	arg1	fractions					479:487	lignin fractions	472:487	lignin fractions	472:487	Films were prepared by mixing lignin fractions as additive in TPS matrix by casting and pressing.					
36610581	6	66	theme	Differential	945:956	arg1	Calorimetry					967:977	Differential Scanning Calorimetry	945:977	Differential Scanning Calorimetry	945:977	Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry).					
36610581	6	66	theme	Differential	945:956	arg1	DSC					940:942	DSC	940:942	DSC (Differential Scanning Calorimetry)	940:978	Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry).					
36610581	5	67	theme	accelerated	680:690	arg1	aging					692:696	accelerated aging	680:696	accelerated aging	680:696	The samples were exposed to accelerated aging with Ultraviolet-C light (UV-C) for 432 h. Structural changes were measured by FTIR (Fourier-Transform Infrared) spectra.					
36610581	5	68	theme	FTIR	777:780	arg1	spectra					811:817	FTIR (Fourier-Transform Infrared) spectra	777:817	FTIR (Fourier-Transform Infrared) spectra	777:817	The samples were exposed to accelerated aging with Ultraviolet-C light (UV-C) for 432 h. Structural changes were measured by FTIR (Fourier-Transform Infrared) spectra.					
36610581	11	69	theme	soluble	1441:1447	arg1	TSOL					1459:1462	TSOL	1459:1462	TSOL	1459:1462	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	69	theme	soluble	1441:1447	arg1	fraction					1449:1456	the soluble fraction	1437:1456	the soluble fraction (TSOL)	1437:1463	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	2	70	from	process	408:414	arg1	EtOAc					434:438	EtOAc	434:438	EtOAc	434:438	KL from Eucalyptus urograndis wood was refined by a sequential fractionation process in ethyl acetate (EtOAc).					
36610581	2	70	from	process	408:414	arg1	acetate					425:431	ethyl acetate	419:431	ethyl acetate (EtOAc)	419:439	KL from Eucalyptus urograndis wood was refined by a sequential fractionation process in ethyl acetate (EtOAc).					
36610581	3	71	theme	TPS	504:506	arg1	matrix					508:513	TPS matrix	504:513	TPS matrix by casting and pressing	504:537	Films were prepared by mixing lignin fractions as additive in TPS matrix by casting and pressing.					
36610581	2	72	theme	sequential	383:392	arg1	process					408:414	a sequential fractionation process	381:414	a sequential fractionation process in ethyl acetate (EtOAc)	381:439	KL from Eucalyptus urograndis wood was refined by a sequential fractionation process in ethyl acetate (EtOAc).					
36610581	1	73	theme	kraft	228:232	arg1	KL					242:243	KL	242:243	KL	242:243	This paper examines the additivation of thermoplastic starch (TPS) matrix by selected fractions of kraft lignin (KL) and correlates its structure-performance when exposed to photooxidative degradation.					
36610581	1	73	theme	kraft	228:232	arg1	lignin					234:239	kraft lignin	228:239	kraft lignin (KL)	228:244	This paper examines the additivation of thermoplastic starch (TPS) matrix by selected fractions of kraft lignin (KL) and correlates its structure-performance when exposed to photooxidative degradation.					
36610581	4	74	from	insoluble	584:592	arg1	INS					604:606	INS	604:606	INS	604:606	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	4	74	from	insoluble	584:592	arg1	EtOAc					597:601	EtOAc	597:601	EtOAc (INS)	597:607	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	6	75	theme	melting	848:854	arg1	temperature					883:893	melting enthalpy, glass transition temperature	848:893	temperature	883:893	Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry).					
36610581	4	76	theme	insoluble	584:592	arg1	KL					581:582	KL	581:582	KL insoluble in EtOAc (INS)	581:607	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	11	77	theme	light	1537:1541	arg1	blocker					1543:1549	an UV-C light blocker	1529:1549	an UV-C light blocker additive on TPS matrix	1529:1572	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	77	theme	light	1537:1541	arg1	effect					1519:1524	effect	1519:1524	stabilizing effect	1507:1524	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	11	77	theme	light	1537:1541	arg1	photoprotection					1487:1501	photoprotection	1487:1501	photoprotection	1487:1501	According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.					
36610581	4	78	theme	soluble	628:634	arg1	KL					625:626	KL	625:626	KL soluble in EtOAc (SOL)	625:649	The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL).					
36610581	1	79	theme	lignin	234:239	arg1	fractions					215:223	selected fractions	206:223	selected fractions of kraft lignin (KL)	206:244	This paper examines the additivation of thermoplastic starch (TPS) matrix by selected fractions of kraft lignin (KL) and correlates its structure-performance when exposed to photooxidative degradation.					
37142296	1	0	theme	hospital	193:200	arg1	clinics					202:208	hospital clinics	193:208	hospital clinics	193:208	Urinary tract infections caused by catheter insertion are prevalent in hospital clinics, which can induce serious complications such as bacteriuria and sepsis, and even lead to patient death.					
37142296	4	1	theme	antibacterial	702:714	arg1	efficiency					716:725	The antibacterial efficiency	698:725	The antibacterial efficiency of the coated catheters against Gram-negative E. coli and Gram-positive S. aureus bacteria	698:816	The antibacterial efficiency of the coated catheters against Gram-negative E. coli and Gram-positive S. aureus bacteria was evaluated with both inhibition zone tests and fluorescence microscopy.					
37142296	6	2	contain	has	1189:1191	arg1	coating					1181:1187	This novel PDA-CMC-AgNPs composite hydrogel coating	1137:1187	This novel PDA-CMC-AgNPs composite hydrogel coating	1137:1187	This novel PDA-CMC-AgNPs composite hydrogel coating has great potential in applications in catheters and other biomedical devices to reduce infections.					
37142296	6	2	contain	has	1189:1191	arg2	potential					1199:1207	great potential	1193:1207	great potential in applications in catheters and other biomedical devices to reduce infections	1193:1286	This novel PDA-CMC-AgNPs composite hydrogel coating has great potential in applications in catheters and other biomedical devices to reduce infections.					
37142296	5	3	theme	dead	1089:1092	arg1	bacteria					1094:1101	dead bacteria	1089:1101	dead bacteria	1089:1101	Compared with the untreated catheter, the PDA-CMC-AgNPs coated catheters showed both good antibacterial and anti-adhesion properties to bacteria, which inhibited the adhesion of live bacteria and dead bacteria by 99.0% and 86.6%, respectively.					
37142296	6	4	theme	biomedical	1248:1257	arg1	devices					1259:1265	other biomedical devices	1242:1265	other biomedical devices	1242:1265	This novel PDA-CMC-AgNPs composite hydrogel coating has great potential in applications in catheters and other biomedical devices to reduce infections.					
37142296	1	5	from	clinics	202:208	arg1	prevalent					180:188	prevalent	180:188	prevalent	180:188	Urinary tract infections caused by catheter insertion are prevalent in hospital clinics, which can induce serious complications such as bacteriuria and sepsis, and even lead to patient death.					
37142296	5	6	theme	anti-adhesion	1001:1013	arg1	properties					1015:1024	both good antibacterial and anti-adhesion properties	973:1024	both good antibacterial and anti-adhesion properties	973:1024	Compared with the untreated catheter, the PDA-CMC-AgNPs coated catheters showed both good antibacterial and anti-adhesion properties to bacteria, which inhibited the adhesion of live bacteria and dead bacteria by 99.0% and 86.6%, respectively.					
37142296	3	7	theme	anti-adhesion	578:590	arg1	properties					592:601	both good antibacterial and anti-adhesion properties	550:601	both good antibacterial and anti-adhesion properties	550:601	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	6	8	theme	great	1193:1197	arg1	potential					1199:1207	great potential	1193:1207	great potential in applications in catheters and other biomedical devices to reduce infections	1193:1286	This novel PDA-CMC-AgNPs composite hydrogel coating has great potential in applications in catheters and other biomedical devices to reduce infections.					
37142296	3	9	theme	good	555:558	arg1	properties					592:601	both good antibacterial and anti-adhesion properties	550:601	both good antibacterial and anti-adhesion properties	550:601	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	1	10	from	prevalent	180:188	arg1	clinics					202:208	hospital clinics	193:208	hospital clinics	193:208	Urinary tract infections caused by catheter insertion are prevalent in hospital clinics, which can induce serious complications such as bacteriuria and sepsis, and even lead to patient death.					
37142296	2	11	theme	poor	387:390	arg1	biocompatibility					392:407	poor biocompatibility	387:407	poor biocompatibility	387:407	The disposable catheters currently used in clinical practice suffer from poor biocompatibility and high infection rate.					
37142296	6	12	from	applications	1212:1223	arg1	devices					1259:1265	other biomedical devices	1242:1265	other biomedical devices	1242:1265	This novel PDA-CMC-AgNPs composite hydrogel coating has great potential in applications in catheters and other biomedical devices to reduce infections.					
37142296	6	12	from	applications	1212:1223	arg1	catheters					1228:1236	catheters	1228:1236	catheters	1228:1236	This novel PDA-CMC-AgNPs composite hydrogel coating has great potential in applications in catheters and other biomedical devices to reduce infections.					
37142296	4	13	theme	coated	734:739	arg1	catheters					741:749	the coated catheters	730:749	the coated catheters against Gram-negative E. coli and Gram-positive S. aureus bacteria	730:816	The antibacterial efficiency of the coated catheters against Gram-negative E. coli and Gram-positive S. aureus bacteria was evaluated with both inhibition zone tests and fluorescence microscopy.					
37142296	6	14	theme	hydrogel	1172:1179	arg1	coating					1181:1187	This novel PDA-CMC-AgNPs composite hydrogel coating	1137:1187	This novel PDA-CMC-AgNPs composite hydrogel coating	1137:1187	This novel PDA-CMC-AgNPs composite hydrogel coating has great potential in applications in catheters and other biomedical devices to reduce infections.					
37142296	4	15	theme	fluorescence	868:879	arg1	microscopy					881:890	fluorescence microscopy	868:890	fluorescence microscopy	868:890	The antibacterial efficiency of the coated catheters against Gram-negative E. coli and Gram-positive S. aureus bacteria was evaluated with both inhibition zone tests and fluorescence microscopy.					
37142296	4	16	theme	Gram-negative	759:771	arg1	bacteria					809:816	Gram-negative E. coli and Gram-positive S. aureus bacteria	759:816	Gram-negative E. coli and Gram-positive S. aureus bacteria	759:816	The antibacterial efficiency of the coated catheters against Gram-negative E. coli and Gram-positive S. aureus bacteria was evaluated with both inhibition zone tests and fluorescence microscopy.					
37142296	4	17	theme	aureus	802:807	arg1	bacteria					809:816	Gram-negative E. coli and Gram-positive S. aureus bacteria	759:816	Gram-negative E. coli and Gram-positive S. aureus bacteria	759:816	The antibacterial efficiency of the coated catheters against Gram-negative E. coli and Gram-positive S. aureus bacteria was evaluated with both inhibition zone tests and fluorescence microscopy.					
37142296	5	18	theme	PDA-CMC-AgNPs	935:947	arg1	catheters					956:964	the PDA-CMC-AgNPs coated catheters	931:964	the PDA-CMC-AgNPs coated catheters	931:964	Compared with the untreated catheter, the PDA-CMC-AgNPs coated catheters showed both good antibacterial and anti-adhesion properties to bacteria, which inhibited the adhesion of live bacteria and dead bacteria by 99.0% and 86.6%, respectively.					
37142296	3	19	with	coating	537:543	arg1	properties					592:601	both good antibacterial and anti-adhesion properties	550:601	both good antibacterial and anti-adhesion properties	550:601	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	5	20	theme	good	978:981	arg1	properties					1015:1024	both good antibacterial and anti-adhesion properties	973:1024	both good antibacterial and anti-adhesion properties	973:1024	Compared with the untreated catheter, the PDA-CMC-AgNPs coated catheters showed both good antibacterial and anti-adhesion properties to bacteria, which inhibited the adhesion of live bacteria and dead bacteria by 99.0% and 86.6%, respectively.					
37142296	3	21	theme	polydopamine	464:475	arg1	coating					537:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating	462:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria	462:613	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	1	22	theme	Urinary	122:128	arg1	infections					136:145	Urinary tract infections	122:145	Urinary tract infections caused by catheter insertion	122:174	Urinary tract infections caused by catheter insertion are prevalent in hospital clinics, which can induce serious complications such as bacteriuria and sepsis, and even lead to patient death.					
37142296	2	23	theme	infection	418:426	arg1	rate					428:431	high infection rate	413:431	high infection rate	413:431	The disposable catheters currently used in clinical practice suffer from poor biocompatibility and high infection rate.					
37142296	1	24	theme	patient	299:305	arg1	death					307:311	patient death	299:311	patient death	299:311	Urinary tract infections caused by catheter insertion are prevalent in hospital clinics, which can induce serious complications such as bacteriuria and sepsis, and even lead to patient death.					
37142296	1	25	theme	tract	130:134	arg1	infections					136:145	Urinary tract infections	122:145	Urinary tract infections caused by catheter insertion	122:174	Urinary tract infections caused by catheter insertion are prevalent in hospital clinics, which can induce serious complications such as bacteriuria and sepsis, and even lead to patient death.					
37142296	2	26	theme	high	413:416	arg1	rate					428:431	high infection rate	413:431	high infection rate	413:431	The disposable catheters currently used in clinical practice suffer from poor biocompatibility and high infection rate.					
37142296	0	27	theme	polydopamine-carboxymethyl	29:54	arg1	coating					91:97	antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating	15:97	antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating	15:97	Fabrication of antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating for urinary catheters.					
37142296	3	28	theme	antibacterial	560:572	arg1	properties					592:601	both good antibacterial and anti-adhesion properties	550:601	both good antibacterial and anti-adhesion properties	550:601	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	1	29	theme	serious	228:234	arg1	sepsis					274:279	sepsis	274:279	sepsis	274:279	Urinary tract infections caused by catheter insertion are prevalent in hospital clinics, which can induce serious complications such as bacteriuria and sepsis, and even lead to patient death.					
37142296	1	29	theme	serious	228:234	arg1	complications					236:248	serious complications	228:248	serious complications such as bacteriuria and sepsis	228:279	Urinary tract infections caused by catheter insertion are prevalent in hospital clinics, which can induce serious complications such as bacteriuria and sepsis, and even lead to patient death.					
37142296	1	29	theme	serious	228:234	arg1	bacteriuria					258:268	bacteriuria	258:268	bacteriuria	258:268	Urinary tract infections caused by catheter insertion are prevalent in hospital clinics, which can induce serious complications such as bacteriuria and sepsis, and even lead to patient death.					
37142296	0	30	theme	antibacterial	15:27	arg1	coating					91:97	antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating	15:97	antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating	15:97	Fabrication of antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating for urinary catheters.					
37142296	5	31	theme	antibacterial	983:995	arg1	properties					1015:1024	both good antibacterial and anti-adhesion properties	973:1024	both good antibacterial and anti-adhesion properties	973:1024	Compared with the untreated catheter, the PDA-CMC-AgNPs coated catheters showed both good antibacterial and anti-adhesion properties to bacteria, which inhibited the adhesion of live bacteria and dead bacteria by 99.0% and 86.6%, respectively.					
37142296	3	32	theme	catheter	661:668	arg1	surfaces					622:629	the surfaces	618:629	the surfaces of a disposable medical latex catheter	618:668	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	2	33	theme	disposable	318:327	arg1	catheters					329:337	The disposable catheters	314:337	The disposable catheters currently used in clinical practice	314:373	The disposable catheters currently used in clinical practice suffer from poor biocompatibility and high infection rate.					
37142296	6	34	from	potential	1199:1207	arg1	applications					1212:1223	applications	1212:1223	applications in catheters and other biomedical devices	1212:1265	This novel PDA-CMC-AgNPs composite hydrogel coating has great potential in applications in catheters and other biomedical devices to reduce infections.					
37142296	3	35	theme	-carboxymethylcellulose	482:504	arg1	coating					537:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating	462:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria	462:613	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	0	36	theme	nanoparticle	69:80	arg1	coating					91:97	antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating	15:97	antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating	15:97	Fabrication of antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating for urinary catheters.					
37142296	1	37	theme	catheter	157:164	arg1	insertion					166:174	catheter insertion	157:174	catheter insertion	157:174	Urinary tract infections caused by catheter insertion are prevalent in hospital clinics, which can induce serious complications such as bacteriuria and sepsis, and even lead to patient death.					
37142296	6	38	theme	composite	1162:1170	arg1	coating					1181:1187	This novel PDA-CMC-AgNPs composite hydrogel coating	1137:1187	This novel PDA-CMC-AgNPs composite hydrogel coating	1137:1187	This novel PDA-CMC-AgNPs composite hydrogel coating has great potential in applications in catheters and other biomedical devices to reduce infections.					
37142296	4	39	theme	inhibition	842:851	arg1	tests					858:862	inhibition zone tests	842:862	inhibition zone tests	842:862	The antibacterial efficiency of the coated catheters against Gram-negative E. coli and Gram-positive S. aureus bacteria was evaluated with both inhibition zone tests and fluorescence microscopy.					
37142296	5	40	theme	untreated	911:919	arg1	catheter					921:928	the untreated catheter	907:928	the untreated catheter	907:928	Compared with the untreated catheter, the PDA-CMC-AgNPs coated catheters showed both good antibacterial and anti-adhesion properties to bacteria, which inhibited the adhesion of live bacteria and dead bacteria by 99.0% and 86.6%, respectively.					
37142296	0	41	theme	cellulose-Ag	56:67	arg1	coating					91:97	antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating	15:97	antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating	15:97	Fabrication of antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating for urinary catheters.					
37142296	3	42	theme	medical	647:653	arg1	catheter					661:668	a disposable medical latex catheter	634:668	a disposable medical latex catheter	634:668	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	5	43	theme	live	1071:1074	arg1	bacteria					1076:1083	live bacteria	1071:1083	live bacteria	1071:1083	Compared with the untreated catheter, the PDA-CMC-AgNPs coated catheters showed both good antibacterial and anti-adhesion properties to bacteria, which inhibited the adhesion of live bacteria and dead bacteria by 99.0% and 86.6%, respectively.					
37142296	3	44	theme	PDA	478:480	arg1	coating					537:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating	462:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria	462:613	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	0	45	theme	coating	91:97	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating for urinary catheters.	0:120	Fabrication of antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating for urinary catheters.					
37142296	3	46	theme	nanoparticles	515:527	arg1	coating					537:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating	462:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria	462:613	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	3	47	theme	latex	655:659	arg1	catheter					661:668	a disposable medical latex catheter	634:668	a disposable medical latex catheter	634:668	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	5	48	theme	bacteria	1076:1083	arg1	adhesion					1059:1066	the adhesion	1055:1066	the adhesion of live bacteria and dead bacteria	1055:1101	Compared with the untreated catheter, the PDA-CMC-AgNPs coated catheters showed both good antibacterial and anti-adhesion properties to bacteria, which inhibited the adhesion of live bacteria and dead bacteria by 99.0% and 86.6%, respectively.					
37142296	6	49	theme	PDA-CMC-AgNPs	1148:1160	arg1	coating					1181:1187	This novel PDA-CMC-AgNPs composite hydrogel coating	1137:1187	This novel PDA-CMC-AgNPs composite hydrogel coating	1137:1187	This novel PDA-CMC-AgNPs composite hydrogel coating has great potential in applications in catheters and other biomedical devices to reduce infections.					
37142296	5	50	theme	coated	949:954	arg1	catheters					956:964	the PDA-CMC-AgNPs coated catheters	931:964	the PDA-CMC-AgNPs coated catheters	931:964	Compared with the untreated catheter, the PDA-CMC-AgNPs coated catheters showed both good antibacterial and anti-adhesion properties to bacteria, which inhibited the adhesion of live bacteria and dead bacteria by 99.0% and 86.6%, respectively.					
37142296	0	51	theme	hydrogel	82:89	arg1	coating					91:97	antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating	15:97	antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating	15:97	Fabrication of antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating for urinary catheters.					
37142296	3	52	theme	dipping	682:688	arg1	method					690:695	a simple dipping method	673:695	a simple dipping method	673:695	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	4	53	theme	zone	853:856	arg1	tests					858:862	inhibition zone tests	842:862	inhibition zone tests	842:862	The antibacterial efficiency of the coated catheters against Gram-negative E. coli and Gram-positive S. aureus bacteria was evaluated with both inhibition zone tests and fluorescence microscopy.					
37142296	3	54	theme	-Ag	511:513	arg1	coating					537:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating	462:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria	462:613	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	5	55	theme	bacteria	1094:1101	arg1	adhesion					1059:1066	the adhesion	1055:1066	the adhesion of live bacteria and dead bacteria	1055:1101	Compared with the untreated catheter, the PDA-CMC-AgNPs coated catheters showed both good antibacterial and anti-adhesion properties to bacteria, which inhibited the adhesion of live bacteria and dead bacteria by 99.0% and 86.6%, respectively.					
37142296	2	56	theme	clinical	357:364	arg1	practice					366:373	clinical practice	357:373	clinical practice	357:373	The disposable catheters currently used in clinical practice suffer from poor biocompatibility and high infection rate.					
37142296	4	57	theme	coli	776:779	arg1	bacteria					809:816	Gram-negative E. coli and Gram-positive S. aureus bacteria	759:816	Gram-negative E. coli and Gram-positive S. aureus bacteria	759:816	The antibacterial efficiency of the coated catheters against Gram-negative E. coli and Gram-positive S. aureus bacteria was evaluated with both inhibition zone tests and fluorescence microscopy.					
37142296	3	58	theme	disposable	636:645	arg1	catheter					661:668	a disposable medical latex catheter	634:668	a disposable medical latex catheter	634:668	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	3	59	theme	CMC	507:509	arg1	coating					537:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating	462:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria	462:613	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	4	60	theme	catheters	741:749	arg1	efficiency					716:725	The antibacterial efficiency	698:725	The antibacterial efficiency of the coated catheters against Gram-negative E. coli and Gram-positive S. aureus bacteria	698:816	The antibacterial efficiency of the coated catheters against Gram-negative E. coli and Gram-positive S. aureus bacteria was evaluated with both inhibition zone tests and fluorescence microscopy.					
37142296	0	61	theme	urinary	103:109	arg1	catheters					111:119	urinary catheters	103:119	urinary catheters	103:119	Fabrication of antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating for urinary catheters.					
37142296	3	62	theme	simple	675:680	arg1	method					690:695	a simple dipping method	673:695	a simple dipping method	673:695	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	6	63	theme	other	1242:1246	arg1	devices					1259:1265	other biomedical devices	1242:1265	other biomedical devices	1242:1265	This novel PDA-CMC-AgNPs composite hydrogel coating has great potential in applications in catheters and other biomedical devices to reduce infections.					
37142296	3	64	theme	AgNPs	530:534	arg1	coating					537:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating	462:543	a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria	462:613	In this paper, we developed a polydopamine (PDA)-carboxymethylcellulose (CMC)-Ag nanoparticles (AgNPs) coating with both good antibacterial and anti-adhesion properties to bacteria on the surfaces of a disposable medical latex catheter by a simple dipping method.					
37142296	6	65	theme	novel	1142:1146	arg1	coating					1181:1187	This novel PDA-CMC-AgNPs composite hydrogel coating	1137:1187	This novel PDA-CMC-AgNPs composite hydrogel coating	1137:1187	This novel PDA-CMC-AgNPs composite hydrogel coating has great potential in applications in catheters and other biomedical devices to reduce infections.					
36906372	10	0	theme	rats	1620:1623	arg1	wounds					1610:1615	the infected full-thickness skin wounds	1577:1615	the infected full-thickness skin wounds of rats	1577:1623	In vivo, BC/PTL/Cu accelerated wound healing and promoted re-epithelialization, collagen deposition, and angiogenesis while inhibiting inflammation of the infected full-thickness skin wounds of rats.					
36906372	9	1	theme	fibroblast	1399:1408	arg1	L929					1420:1423	mouse fibroblast cell line L929	1393:1423	mouse fibroblast cell line L929	1393:1423	By controlling copper concentration, BC/PTL/Cu were not cytotoxic to mouse fibroblast cell line L929.					
36906372	1	2	theme	wound	286:290	arg1	healing					292:298	delayed wound healing	278:298	delayed wound healing	278:298	Bacterial overgrowth in injured wounds causes wound infection and excessive inflammation, leading to delayed wound healing.					
36906372	0	3	theme	Antibacterial	85:97	arg1	cellulose					74:82	bacterial cellulose	64:82	bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing	64:174	Fabrication of Cu2+-loaded phase-transited lysozyme nanofilm on bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing.					
36906372	1	4	from	overgrowth	187:196	arg1	wounds					209:214	injured wounds	201:214	injured wounds	201:214	Bacterial overgrowth in injured wounds causes wound infection and excessive inflammation, leading to delayed wound healing.					
36906372	2	5	theme	bacterial	393:401	arg1	growth					403:408	bacterial growth	393:408	bacterial growth	393:408	Successful treatment of delayed infected wound healing demands dressings, which can inhibit bacterial growth and inflammation and simultaneously induce vascularization, collagen deposition, and re-epithelialization of wounds.					
36906372	10	6	theme	wounds	1610:1615	arg1	inflammation					1561:1572	inflammation	1561:1572	inflammation of the infected full-thickness skin wounds of rats	1561:1623	In vivo, BC/PTL/Cu accelerated wound healing and promoted re-epithelialization, collagen deposition, and angiogenesis while inhibiting inflammation of the infected full-thickness skin wounds of rats.					
36906372	0	7	from	Fabrication	0:10	arg1	cellulose					74:82	bacterial cellulose	64:82	bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing	64:174	Fabrication of Cu2+-loaded phase-transited lysozyme nanofilm on bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing.					
36906372	11	8	theme	BC/PTL/Cu	1671:1679	arg1	composites					1681:1690	BC/PTL/Cu composites	1671:1690	BC/PTL/Cu composites	1671:1690	Collectively, these results demonstrate that BC/PTL/Cu composites are promising dressings for healing infected wounds.					
36906372	11	8	theme	BC/PTL/Cu	1671:1679	arg1	dressings					1706:1714	promising dressings	1696:1714	promising dressings for healing infected wounds	1696:1742	Collectively, these results demonstrate that BC/PTL/Cu composites are promising dressings for healing infected wounds.					
36906372	2	9	theme	infected	333:340	arg1	healing					348:354	delayed infected wound healing	325:354	delayed infected wound healing	325:354	Successful treatment of delayed infected wound healing demands dressings, which can inhibit bacterial growth and inflammation and simultaneously induce vascularization, collagen deposition, and re-epithelialization of wounds.					
36906372	9	10	theme	mouse	1393:1397	arg1	L929					1420:1423	mouse fibroblast cell line L929	1393:1423	mouse fibroblast cell line L929	1393:1423	By controlling copper concentration, BC/PTL/Cu were not cytotoxic to mouse fibroblast cell line L929.					
36906372	0	11	theme	anti-inflammatory	100:116	arg1	cellulose					74:82	bacterial cellulose	64:82	bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing	64:174	Fabrication of Cu2+-loaded phase-transited lysozyme nanofilm on bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing.					
36906372	5	12	from	break	873:877	arg1	at					870:871	the elongation at break	855:877	the elongation at break	855:877	The tensile strength and the elongation at break of the membranes were not significantly changed after modification with PTL and Cu2+.					
36906372	5	12	from	break	873:877	arg1	elongation					859:868	the elongation at break	855:877	the elongation at break	855:877	The tensile strength and the elongation at break of the membranes were not significantly changed after modification with PTL and Cu2+.					
36906372	4	13	theme	electrostatic	802:814	arg1	coordination					816:827	electrostatic coordination	802:827	electrostatic coordination	802:827	The results confirm that PTL were successfully self-assembled on BC matrix, and Cu2+ were loaded into PTL through electrostatic coordination.					
36906372	2	14	theme	delayed	325:331	arg1	healing					348:354	delayed infected wound healing	325:354	delayed infected wound healing	325:354	Successful treatment of delayed infected wound healing demands dressings, which can inhibit bacterial growth and inflammation and simultaneously induce vascularization, collagen deposition, and re-epithelialization of wounds.					
36906372	11	15	theme	promising	1696:1704	arg1	composites					1681:1690	BC/PTL/Cu composites	1671:1690	BC/PTL/Cu composites	1671:1690	Collectively, these results demonstrate that BC/PTL/Cu composites are promising dressings for healing infected wounds.					
36906372	11	15	theme	promising	1696:1704	arg1	dressings					1706:1714	promising dressings	1696:1714	promising dressings for healing infected wounds	1696:1742	Collectively, these results demonstrate that BC/PTL/Cu composites are promising dressings for healing infected wounds.					
36906372	0	16	theme	pro-angiogenesis	123:138	arg1	cellulose					74:82	bacterial cellulose	64:82	bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing	64:174	Fabrication of Cu2+-loaded phase-transited lysozyme nanofilm on bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing.					
36906372	9	17	theme	cell	1410:1413	arg1	L929					1420:1423	mouse fibroblast cell line L929	1393:1423	mouse fibroblast cell line L929	1393:1423	By controlling copper concentration, BC/PTL/Cu were not cytotoxic to mouse fibroblast cell line L929.					
36906372	10	18	theme	full-thickness	1590:1603	arg1	wounds					1610:1615	the infected full-thickness skin wounds	1577:1615	the infected full-thickness skin wounds of rats	1577:1623	In vivo, BC/PTL/Cu accelerated wound healing and promoted re-epithelialization, collagen deposition, and angiogenesis while inhibiting inflammation of the infected full-thickness skin wounds of rats.					
36906372	5	19	theme	elongation	859:868	arg1	break					873:877	the elongation at break	855:877	the elongation at break	855:877	The tensile strength and the elongation at break of the membranes were not significantly changed after modification with PTL and Cu2+.					
36906372	3	20	theme	Cu2+-loaded	582:592	arg1	BC/PTL/Cu					635:643	BC/PTL/Cu	635:643	BC/PTL/Cu	635:643	In this study, bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds.					
36906372	3	20	theme	Cu2+-loaded	582:592	arg1	nanofilm					625:632	Cu2+-loaded phase-transited lysozyme (PTL) nanofilm	582:632	Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu)	582:644	In this study, bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds.					
36906372	1	21	theme	injured	201:207	arg1	wounds					209:214	injured wounds	201:214	injured wounds	201:214	Bacterial overgrowth in injured wounds causes wound infection and excessive inflammation, leading to delayed wound healing.					
36906372	9	22	theme	line	1415:1418	arg1	L929					1420:1423	mouse fibroblast cell line L929	1393:1423	mouse fibroblast cell line L929	1393:1423	By controlling copper concentration, BC/PTL/Cu were not cytotoxic to mouse fibroblast cell line L929.					
36906372	5	23	theme	tensile	834:840	arg1	strength					842:849	tensile strength	834:849	tensile strength	834:849	The tensile strength and the elongation at break of the membranes were not significantly changed after modification with PTL and Cu2+.					
36906372	10	24	theme	skin	1605:1608	arg1	wounds					1610:1615	the infected full-thickness skin wounds	1577:1615	the infected full-thickness skin wounds of rats	1577:1623	In vivo, BC/PTL/Cu accelerated wound healing and promoted re-epithelialization, collagen deposition, and angiogenesis while inhibiting inflammation of the infected full-thickness skin wounds of rats.					
36906372	3	25	theme	phase-transited	594:608	arg1	BC/PTL/Cu					635:643	BC/PTL/Cu	635:643	BC/PTL/Cu	635:643	In this study, bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds.					
36906372	3	25	theme	phase-transited	594:608	arg1	nanofilm					625:632	Cu2+-loaded phase-transited lysozyme (PTL) nanofilm	582:632	Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu)	582:644	In this study, bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds.					
36906372	2	26	theme	wounds	519:524	arg1	vascularization					453:467	vascularization	453:467	vascularization	453:467	Successful treatment of delayed infected wound healing demands dressings, which can inhibit bacterial growth and inflammation and simultaneously induce vascularization, collagen deposition, and re-epithelialization of wounds.					
36906372	2	26	theme	wounds	519:524	arg1	deposition					479:488	collagen deposition	470:488	collagen deposition	470:488	Successful treatment of delayed infected wound healing demands dressings, which can inhibit bacterial growth and inflammation and simultaneously induce vascularization, collagen deposition, and re-epithelialization of wounds.					
36906372	2	26	theme	wounds	519:524	arg1	re-epithelialization					495:514	re-epithelialization	495:514	re-epithelialization	495:514	Successful treatment of delayed infected wound healing demands dressings, which can inhibit bacterial growth and inflammation and simultaneously induce vascularization, collagen deposition, and re-epithelialization of wounds.					
36906372	5	27	theme	at	870:871	arg1	break					873:877	the elongation at break	855:877	the elongation at break	855:877	The tensile strength and the elongation at break of the membranes were not significantly changed after modification with PTL and Cu2+.					
36906372	5	28	dep	strength	842:849	arg1	The					830:832	The	830:832	The	830:832	The tensile strength and the elongation at break of the membranes were not significantly changed after modification with PTL and Cu2+.					
36906372	1	29	theme	wound	223:227	arg1	infection					229:237	wound infection	223:237	wound infection	223:237	Bacterial overgrowth in injured wounds causes wound infection and excessive inflammation, leading to delayed wound healing.					
36906372	0	30	theme	phase-transited	27:41	arg1	nanofilm					52:59	Cu2+-loaded phase-transited lysozyme nanofilm	15:59	Cu2+-loaded phase-transited lysozyme nanofilm	15:59	Fabrication of Cu2+-loaded phase-transited lysozyme nanofilm on bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing.					
36906372	0	31	theme	bacteria-infected	144:160	arg1	healing					168:174	bacteria-infected wound healing	144:174	bacteria-infected wound healing	144:174	Fabrication of Cu2+-loaded phase-transited lysozyme nanofilm on bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing.					
36906372	10	32	theme	collagen	1506:1513	arg1	deposition					1515:1524	collagen deposition	1506:1524	collagen deposition	1506:1524	In vivo, BC/PTL/Cu accelerated wound healing and promoted re-epithelialization, collagen deposition, and angiogenesis while inhibiting inflammation of the infected full-thickness skin wounds of rats.					
36906372	2	33	theme	healing	348:354	arg1	treatment					312:320	Successful treatment	301:320	Successful treatment of delayed infected wound healing	301:354	Successful treatment of delayed infected wound healing demands dressings, which can inhibit bacterial growth and inflammation and simultaneously induce vascularization, collagen deposition, and re-epithelialization of wounds.					
36906372	0	34	theme	Cu2+-loaded	15:25	arg1	nanofilm					52:59	Cu2+-loaded phase-transited lysozyme nanofilm	15:59	Cu2+-loaded phase-transited lysozyme nanofilm	15:59	Fabrication of Cu2+-loaded phase-transited lysozyme nanofilm on bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing.					
36906372	6	35	theme	BC/PTL/Cu	1008:1016	arg1	roughness					995:1003	the surface roughness	983:1003	the surface roughness of BC/PTL/Cu	983:1016	Compared with BC, the surface roughness of BC/PTL/Cu significantly increased while the hydrophilicity decreased.					
36906372	2	36	theme	wound	342:346	arg1	healing					348:354	delayed infected wound healing	325:354	delayed infected wound healing	325:354	Successful treatment of delayed infected wound healing demands dressings, which can inhibit bacterial growth and inflammation and simultaneously induce vascularization, collagen deposition, and re-epithelialization of wounds.					
36906372	5	37	theme	membranes	886:894	arg1	break					873:877	the elongation at break	855:877	the elongation at break	855:877	The tensile strength and the elongation at break of the membranes were not significantly changed after modification with PTL and Cu2+.					
36906372	5	37	theme	membranes	886:894	arg1	strength					842:849	tensile strength	834:849	tensile strength	834:849	The tensile strength and the elongation at break of the membranes were not significantly changed after modification with PTL and Cu2+.					
36906372	0	38	theme	nanofilm	52:59	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of Cu2+-loaded phase-transited lysozyme nanofilm on bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing.	0:175	Fabrication of Cu2+-loaded phase-transited lysozyme nanofilm on bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing.					
36906372	4	39	theme	BC	753:754	arg1	matrix					756:761	BC matrix	753:761	BC matrix	753:761	The results confirm that PTL were successfully self-assembled on BC matrix, and Cu2+ were loaded into PTL through electrostatic coordination.					
36906372	1	40	theme	excessive	243:251	arg1	inflammation					253:264	excessive inflammation	243:264	excessive inflammation	243:264	Bacterial overgrowth in injured wounds causes wound infection and excessive inflammation, leading to delayed wound healing.					
36906372	2	41	theme	collagen	470:477	arg1	deposition					479:488	collagen deposition	470:488	collagen deposition	470:488	Successful treatment of delayed infected wound healing demands dressings, which can inhibit bacterial growth and inflammation and simultaneously induce vascularization, collagen deposition, and re-epithelialization of wounds.					
36906372	0	42	theme	lysozyme	43:50	arg1	nanofilm					52:59	Cu2+-loaded phase-transited lysozyme nanofilm	15:59	Cu2+-loaded phase-transited lysozyme nanofilm	15:59	Fabrication of Cu2+-loaded phase-transited lysozyme nanofilm on bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing.					
36906372	0	43	theme	wound	162:166	arg1	healing					168:174	bacteria-infected wound healing	144:174	bacteria-infected wound healing	144:174	Fabrication of Cu2+-loaded phase-transited lysozyme nanofilm on bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing.					
36906372	11	44	theme	infected	1728:1735	arg1	wounds					1737:1742	healing infected wounds	1720:1742	healing infected wounds	1720:1742	Collectively, these results demonstrate that BC/PTL/Cu composites are promising dressings for healing infected wounds.					
36906372	0	45	theme	bacterial	64:72	arg1	cellulose					74:82	bacterial cellulose	64:82	bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing	64:174	Fabrication of Cu2+-loaded phase-transited lysozyme nanofilm on bacterial cellulose: Antibacterial, anti-inflammatory, and pro-angiogenesis for bacteria-infected wound healing.					
36906372	6	46	theme	surface	987:993	arg1	roughness					995:1003	the surface roughness	983:1003	the surface roughness of BC/PTL/Cu	983:1016	Compared with BC, the surface roughness of BC/PTL/Cu significantly increased while the hydrophilicity decreased.					
36906372	7	47	theme	release	1115:1121	arg1	rate					1123:1126	slower release rate	1108:1126	slower release rate of Cu2+	1108:1134	Moreover, BC/PTL/Cu displayed slower release rate of Cu2+ compared with BC directly loaded with Cu2+.					
36906372	9	48	theme	copper	1339:1344	arg1	concentration					1346:1358	copper concentration	1339:1358	copper concentration	1339:1358	By controlling copper concentration, BC/PTL/Cu were not cytotoxic to mouse fibroblast cell line L929.					
36906372	7	49	theme	Cu2+	1131:1134	arg1	rate					1123:1126	slower release rate	1108:1126	slower release rate of Cu2+	1108:1134	Moreover, BC/PTL/Cu displayed slower release rate of Cu2+ compared with BC directly loaded with Cu2+.					
36906372	5	50	with	modification	933:944	arg1	Cu2+					959:962	Cu2+	959:962	Cu2+	959:962	The tensile strength and the elongation at break of the membranes were not significantly changed after modification with PTL and Cu2+.					
36906372	5	50	with	modification	933:944	arg1	PTL					951:953	PTL	951:953	PTL	951:953	The tensile strength and the elongation at break of the membranes were not significantly changed after modification with PTL and Cu2+.					
36906372	10	51	theme	infected	1581:1588	arg1	wounds					1610:1615	the infected full-thickness skin wounds	1577:1615	the infected full-thickness skin wounds of rats	1577:1623	In vivo, BC/PTL/Cu accelerated wound healing and promoted re-epithelialization, collagen deposition, and angiogenesis while inhibiting inflammation of the infected full-thickness skin wounds of rats.					
36906372	3	52	theme	infected	671:678	arg1	wounds					680:685	healing infected wounds	663:685	healing infected wounds	663:685	In this study, bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds.					
36906372	2	53	theme	Successful	301:310	arg1	treatment					312:320	Successful treatment	301:320	Successful treatment of delayed infected wound healing	301:354	Successful treatment of delayed infected wound healing demands dressings, which can inhibit bacterial growth and inflammation and simultaneously induce vascularization, collagen deposition, and re-epithelialization of wounds.					
36906372	11	54	theme	healing	1720:1726	arg1	wounds					1737:1742	healing infected wounds	1720:1742	healing infected wounds	1720:1742	Collectively, these results demonstrate that BC/PTL/Cu composites are promising dressings for healing infected wounds.					
36906372	3	55	theme	PTL	620:622	arg1	BC/PTL/Cu					635:643	BC/PTL/Cu	635:643	BC/PTL/Cu	635:643	In this study, bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds.					
36906372	3	55	theme	PTL	620:622	arg1	nanofilm					625:632	Cu2+-loaded phase-transited lysozyme (PTL) nanofilm	582:632	Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu)	582:644	In this study, bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds.					
36906372	7	56	theme	slower	1108:1113	arg1	rate					1123:1126	slower release rate	1108:1126	slower release rate of Cu2+	1108:1134	Moreover, BC/PTL/Cu displayed slower release rate of Cu2+ compared with BC directly loaded with Cu2+.					
36906372	3	57	theme	lysozyme	610:617	arg1	BC/PTL/Cu					635:643	BC/PTL/Cu	635:643	BC/PTL/Cu	635:643	In this study, bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds.					
36906372	3	57	theme	lysozyme	610:617	arg1	nanofilm					625:632	Cu2+-loaded phase-transited lysozyme (PTL) nanofilm	582:632	Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu)	582:644	In this study, bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds.					
36906372	3	58	theme	bacterial	542:550	arg1	cellulose					552:560	bacterial cellulose	542:560	bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu)	542:644	In this study, bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds.					
36906372	3	58	theme	bacterial	542:550	arg1	BC					563:564	BC	563:564	BC	563:564	In this study, bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds.					
36906372	8	59	theme	antibacterial	1205:1217	arg1	activity					1219:1226	good antibacterial activity	1200:1226	good antibacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa	1200:1321	BC/PTL/Cu exhibited good antibacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa.					
36906372	10	60	theme	wound	1457:1461	arg1	healing					1463:1469	wound healing	1457:1469	wound healing	1457:1469	In vivo, BC/PTL/Cu accelerated wound healing and promoted re-epithelialization, collagen deposition, and angiogenesis while inhibiting inflammation of the infected full-thickness skin wounds of rats.					
36906372	8	61	theme	good	1200:1203	arg1	activity					1219:1226	good antibacterial activity	1200:1226	good antibacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa	1200:1321	BC/PTL/Cu exhibited good antibacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa.					
36906372	3	62	theme	healing	663:669	arg1	wounds					680:685	healing infected wounds	663:685	healing infected wounds	663:685	In this study, bacterial cellulose (BC) deposited with Cu2+-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds.					
36906372	1	63	theme	Bacterial	177:185	arg1	overgrowth					187:196	Bacterial overgrowth	177:196	Bacterial overgrowth in injured wounds	177:214	Bacterial overgrowth in injured wounds causes wound infection and excessive inflammation, leading to delayed wound healing.					
36906372	1	64	theme	delayed	278:284	arg1	healing					292:298	delayed wound healing	278:298	delayed wound healing	278:298	Bacterial overgrowth in injured wounds causes wound infection and excessive inflammation, leading to delayed wound healing.					
36907301	0	0	theme	isolated	77:84	arg1	starch					91:96	isolated corn starch	77:96	isolated corn starch	77:96	The effect of the electromagnetic field on the physicochemical properties of isolated corn starch obtained of plants from irradiate seeds.					
36907301	3	1	theme	industrial	433:442	arg1	importance					444:453	the great industrial importance	423:453	the great industrial importance of starch	423:463	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	0	2	from	effect	4:9	arg1	properties					63:72	the physicochemical properties	43:72	the physicochemical properties of isolated corn starch	43:96	The effect of the electromagnetic field on the physicochemical properties of isolated corn starch obtained of plants from irradiate seeds.					
36907301	7	3	theme	pasting	1008:1014	arg1	profile					1016:1022	the pasting profile	1004:1022	the pasting profile of starch	1004:1032	However, the pasting profile of starch was affected, and a decrease in the peak viscosity was obtained when the intensity of EMF increased.					
36907301	6	4	theme	EMF	990:992	arg1	intensity					977:985	the intensity	973:985	the intensity of EMF	973:992	The X-ray patterns showed that the orthorhombic structure was kept constant, unaffected by the intensity of EMF.					
36907301	6	5	theme	X-ray	886:890	arg1	patterns					892:899	The X-ray patterns	882:899	The X-ray patterns	882:899	The X-ray patterns showed that the orthorhombic structure was kept constant, unaffected by the intensity of EMF.					
36907301	0	6	theme	starch	91:96	arg1	properties					63:72	the physicochemical properties	43:72	the physicochemical properties of isolated corn starch	43:96	The effect of the electromagnetic field on the physicochemical properties of isolated corn starch obtained of plants from irradiate seeds.					
36907301	8	7	theme	bonds	1254:1258	arg1	stretching					1233:1242	the stretching	1229:1242	the stretching of the CO bonds at wave number 1.711 cm-1	1229:1284	In contrast to the control plants, FTIR shows characteristic bands which can be attributed to the stretching of the CO bonds at wave number 1.711 cm-1.					
36907301	4	8	dep	μT	620:621	arg1	15 days					627:633	15 days	627:633	15 days	627:633	Mother seed were exposed to three different intensities 23, 70, and 118 μT for 15 days.					
36907301	8	9	theme	number	1268:1273	arg1	1.711 cm-1					1275:1284	wave number 1.711 cm-1	1263:1284	wave number 1.711 cm-1	1263:1284	In contrast to the control plants, FTIR shows characteristic bands which can be attributed to the stretching of the CO bonds at wave number 1.711 cm-1.					
36907301	0	10	theme	corn	86:89	arg1	starch					91:96	isolated corn starch	77:96	isolated corn starch	77:96	The effect of the electromagnetic field on the physicochemical properties of isolated corn starch obtained of plants from irradiate seeds.					
36907301	5	11	theme	electron	860:867	arg1	microscopy					869:878	scanning electron microscopy	851:878	scanning electron microscopy	851:878	Except for a slight porosity on the surface of the starch of the grains of plants exposed to higher EMF, the starch showed no morphological differences between the different treatments and the control (according to scanning electron microscopy).					
36907301	8	12	theme	control	1154:1160	arg1	plants					1162:1167	the control plants	1150:1167	the control plants	1150:1167	In contrast to the control plants, FTIR shows characteristic bands which can be attributed to the stretching of the CO bonds at wave number 1.711 cm-1.					
36907301	5	13	theme	slight	649:654	arg1	porosity					656:663	a slight porosity	647:663	a slight porosity on the surface of the starch of the grains of plants exposed to higher EMF	647:738	Except for a slight porosity on the surface of the starch of the grains of plants exposed to higher EMF, the starch showed no morphological differences between the different treatments and the control (according to scanning electron microscopy).					
36907301	2	14	theme	electromagnetic	240:254	arg1	fields					256:261	electromagnetic fields	240:261	electromagnetic fields (EMF)	240:267	Consequently, any treatment, such as electromagnetic fields (EMF) must alter these components, which in turn alters the physicochemical properties of the grain.					
36907301	2	14	theme	electromagnetic	240:254	arg1	EMF					264:266	EMF	264:266	EMF	264:266	Consequently, any treatment, such as electromagnetic fields (EMF) must alter these components, which in turn alters the physicochemical properties of the grain.					
36907301	3	15	theme	major	382:386	arg1	component					388:396	a major component	380:396	a major component of corn grain	380:410	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	3	15	theme	major	382:386	arg1	starch					370:375	starch	370:375	starch	370:375	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	7	16	theme	starch	1027:1032	arg1	profile					1016:1022	the pasting profile	1004:1022	the pasting profile of starch	1004:1032	However, the pasting profile of starch was affected, and a decrease in the peak viscosity was obtained when the intensity of EMF increased.					
36907301	5	17	theme	grains	701:706	arg1	starch					687:692	the starch	683:692	the starch of the grains of plants exposed to higher EMF	683:738	Except for a slight porosity on the surface of the starch of the grains of plants exposed to higher EMF, the starch showed no morphological differences between the different treatments and the control (according to scanning electron microscopy).					
36907301	6	18	theme	orthorhombic	917:928	arg1	unaffected					959:968	unaffected	959:968	unaffected by the intensity of EMF	959:992	The X-ray patterns showed that the orthorhombic structure was kept constant, unaffected by the intensity of EMF.					
36907301	6	18	theme	orthorhombic	917:928	arg1	structure					930:938	the orthorhombic structure	913:938	the orthorhombic structure	913:938	The X-ray patterns showed that the orthorhombic structure was kept constant, unaffected by the intensity of EMF.					
36907301	6	18	theme	orthorhombic	917:928	arg1	constant					949:956	constant	949:956	constant	949:956	The X-ray patterns showed that the orthorhombic structure was kept constant, unaffected by the intensity of EMF.					
36907301	8	19	from	1.711 cm-1	1275:1284	arg1	bonds					1254:1258	the CO bonds	1247:1258	the CO bonds at wave number 1.711 cm-1	1247:1284	In contrast to the control plants, FTIR shows characteristic bands which can be attributed to the stretching of the CO bonds at wave number 1.711 cm-1.					
36907301	8	19	from	1.711 cm-1	1275:1284	arg1	stretching					1233:1242	the stretching	1229:1242	the stretching of the CO bonds at wave number 1.711 cm-1	1229:1284	In contrast to the control plants, FTIR shows characteristic bands which can be attributed to the stretching of the CO bonds at wave number 1.711 cm-1.					
36907301	2	20	theme	physicochemical	323:337	arg1	properties					339:348	the physicochemical properties	319:348	the physicochemical properties of the grain	319:361	Consequently, any treatment, such as electromagnetic fields (EMF) must alter these components, which in turn alters the physicochemical properties of the grain.					
36907301	5	21	theme	plants	711:716	arg1	grains					701:706	the grains	697:706	the grains of plants exposed to higher EMF	697:738	Except for a slight porosity on the surface of the starch of the grains of plants exposed to higher EMF, the starch showed no morphological differences between the different treatments and the control (according to scanning electron microscopy).					
36907301	3	22	theme	grain	406:410	arg1	importance					444:453	the great industrial importance	423:453	the great industrial importance of starch	423:463	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	3	22	theme	grain	406:410	arg1	component					388:396	a major component	380:396	a major component of corn grain	380:410	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	3	22	theme	grain	406:410	arg1	starch					370:375	starch	370:375	starch	370:375	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	9	23	theme	physical	1311:1318	arg1	modification					1320:1331	a physical modification	1309:1331	a physical modification of starch	1309:1341	EMF can be considered a physical modification of starch.					
36907301	4	24	theme	Mother	548:553	arg1	seed					555:558	Mother seed	548:558	Mother seed	548:558	Mother seed were exposed to three different intensities 23, 70, and 118 μT for 15 days.					
36907301	7	25	from	decrease	1054:1061	arg1	viscosity					1075:1083	the peak viscosity	1066:1083	the peak viscosity	1066:1083	However, the pasting profile of starch was affected, and a decrease in the peak viscosity was obtained when the intensity of EMF increased.					
36907301	5	26	theme	higher	729:734	arg1	EMF					736:738	higher EMF	729:738	higher EMF	729:738	Except for a slight porosity on the surface of the starch of the grains of plants exposed to higher EMF, the starch showed no morphological differences between the different treatments and the control (according to scanning electron microscopy).					
36907301	0	27	theme	field	34:38	arg1	effect					4:9	The effect	0:9	The effect of the electromagnetic field on the physicochemical properties of isolated corn starch	0:96	The effect of the electromagnetic field on the physicochemical properties of isolated corn starch obtained of plants from irradiate seeds.					
36907301	9	28	theme	starch	1336:1341	arg1	modification					1320:1331	a physical modification	1309:1331	a physical modification of starch	1309:1341	EMF can be considered a physical modification of starch.					
36907301	5	29	from	porosity	656:663	arg1	surface					672:678	the surface	668:678	the surface of the starch of the grains of plants exposed to higher EMF	668:738	Except for a slight porosity on the surface of the starch of the grains of plants exposed to higher EMF, the starch showed no morphological differences between the different treatments and the control (according to scanning electron microscopy).					
36907301	4	30	theme	different	582:590	arg1	intensities					592:602	three different intensities	576:602	three different intensities	576:602	Mother seed were exposed to three different intensities 23, 70, and 118 μT for 15 days.					
36907301	0	31	theme	electromagnetic	18:32	arg1	field					34:38	the electromagnetic field	14:38	the electromagnetic field	14:38	The effect of the electromagnetic field on the physicochemical properties of isolated corn starch obtained of plants from irradiate seeds.					
36907301	0	32	theme	irradiate	122:130	arg1	seeds					132:136	irradiate seeds	122:136	irradiate seeds	122:136	The effect of the electromagnetic field on the physicochemical properties of isolated corn starch obtained of plants from irradiate seeds.					
36907301	3	33	theme	starch	540:545	arg1	properties					526:535	the physicochemical properties	506:535	the physicochemical properties of starch	506:545	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	2	34	theme	grain	357:361	arg1	properties					339:348	the physicochemical properties	319:348	the physicochemical properties of the grain	319:361	Consequently, any treatment, such as electromagnetic fields (EMF) must alter these components, which in turn alters the physicochemical properties of the grain.					
36907301	3	35	theme	physicochemical	510:524	arg1	properties					526:535	the physicochemical properties	506:535	the physicochemical properties of starch	506:545	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	5	36	theme	different	800:808	arg1	treatments					810:819	the different treatments	796:819	the different treatments	796:819	Except for a slight porosity on the surface of the starch of the grains of plants exposed to higher EMF, the starch showed no morphological differences between the different treatments and the control (according to scanning electron microscopy).					
36907301	6	37	dep	constant	949:956	arg1	unaffected					959:968	unaffected	959:968	unaffected by the intensity of EMF	959:992	The X-ray patterns showed that the orthorhombic structure was kept constant, unaffected by the intensity of EMF.					
36907301	6	37	dep	constant	949:956	arg1	structure					930:938	the orthorhombic structure	913:938	the orthorhombic structure	913:938	The X-ray patterns showed that the orthorhombic structure was kept constant, unaffected by the intensity of EMF.					
36907301	6	37	dep	constant	949:956	arg1	constant					949:956	constant	949:956	constant	949:956	The X-ray patterns showed that the orthorhombic structure was kept constant, unaffected by the intensity of EMF.					
36907301	8	38	theme	CO	1251:1252	arg1	bonds					1254:1258	the CO bonds	1247:1258	the CO bonds at wave number 1.711 cm-1	1247:1284	In contrast to the control plants, FTIR shows characteristic bands which can be attributed to the stretching of the CO bonds at wave number 1.711 cm-1.					
36907301	3	39	theme	great	427:431	arg1	importance					444:453	the great industrial importance	423:453	the great industrial importance of starch	423:463	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	7	40	theme	EMF	1120:1122	arg1	intensity					1107:1115	the intensity	1103:1115	the intensity of EMF	1103:1122	However, the pasting profile of starch was affected, and a decrease in the peak viscosity was obtained when the intensity of EMF increased.					
36907301	8	41	dep	plants	1162:1167	arg1	contrast					1138:1145	contrast	1138:1145	contrast	1138:1145	In contrast to the control plants, FTIR shows characteristic bands which can be attributed to the stretching of the CO bonds at wave number 1.711 cm-1.					
36907301	8	42	theme	wave	1263:1266	arg1	1.711 cm-1					1275:1284	wave number 1.711 cm-1	1263:1284	wave number 1.711 cm-1	1263:1284	In contrast to the control plants, FTIR shows characteristic bands which can be attributed to the stretching of the CO bonds at wave number 1.711 cm-1.					
36907301	0	43	from	seeds	132:136	arg1	plants					110:115	plants	110:115	plants from irradiate seeds	110:136	The effect of the electromagnetic field on the physicochemical properties of isolated corn starch obtained of plants from irradiate seeds.					
36907301	5	44	theme	scanning	851:858	arg1	microscopy					869:878	scanning electron microscopy	851:878	scanning electron microscopy	851:878	Except for a slight porosity on the surface of the starch of the grains of plants exposed to higher EMF, the starch showed no morphological differences between the different treatments and the control (according to scanning electron microscopy).					
36907301	1	45	theme	Maize	139:143	arg1	grains					145:150	Maize grains	139:150	Maize grains	139:150	Maize grains are composed of the pericarp, endosperm, and germ.					
36907301	8	46	theme	characteristic	1181:1194	arg1	bands					1196:1200	characteristic bands	1181:1200	characteristic bands which can be attributed to the stretching of the CO bonds at wave number 1.711 cm-1	1181:1284	In contrast to the control plants, FTIR shows characteristic bands which can be attributed to the stretching of the CO bonds at wave number 1.711 cm-1.					
36907301	3	47	theme	corn	401:404	arg1	grain					406:410	corn grain	401:410	corn grain	401:410	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	5	48	theme	morphological	762:774	arg1	differences					776:786	no morphological differences	759:786	no morphological differences between the different treatments and the control (according to scanning electron microscopy)	759:879	Except for a slight porosity on the surface of the starch of the grains of plants exposed to higher EMF, the starch showed no morphological differences between the different treatments and the control (according to scanning electron microscopy).					
36907301	0	49	theme	physicochemical	47:61	arg1	properties					63:72	the physicochemical properties	43:72	the physicochemical properties of isolated corn starch	43:96	The effect of the electromagnetic field on the physicochemical properties of isolated corn starch obtained of plants from irradiate seeds.					
36907301	5	50	theme	starch	687:692	arg1	surface					672:678	the surface	668:678	the surface of the starch of the grains of plants exposed to higher EMF	668:738	Except for a slight porosity on the surface of the starch of the grains of plants exposed to higher EMF, the starch showed no morphological differences between the different treatments and the control (according to scanning electron microscopy).					
36907301	3	51	theme	starch	458:463	arg1	importance					444:453	the great industrial importance	423:453	the great industrial importance of starch	423:463	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	3	51	theme	starch	458:463	arg1	component					388:396	a major component	380:396	a major component of corn grain	380:410	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	3	51	theme	starch	458:463	arg1	starch					370:375	starch	370:375	starch	370:375	Since starch is a major component of corn grain, and given the great industrial importance of starch, this study investigates how EMF affects the physicochemical properties of starch.					
36907301	8	52	from	stretching	1233:1242	arg1	1.711 cm-1					1275:1284	wave number 1.711 cm-1	1263:1284	wave number 1.711 cm-1	1263:1284	In contrast to the control plants, FTIR shows characteristic bands which can be attributed to the stretching of the CO bonds at wave number 1.711 cm-1.					
36907301	7	53	theme	peak	1070:1073	arg1	viscosity					1075:1083	the peak viscosity	1066:1083	the peak viscosity	1066:1083	However, the pasting profile of starch was affected, and a decrease in the peak viscosity was obtained when the intensity of EMF increased.					
36433128	6	0	theme	cerium	1258:1263	arg1	oxide					1265:1269	cerium oxide	1258:1269	cerium oxide (reversible transitions between the states Ce4+ and Ce3+)	1258:1327	The latter effect is considered to be caused by the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films.					
36433128	3	1	theme	cerium	552:557	arg1	oxide					559:563	citrate-stabilized cerium oxide	533:563	citrate-stabilized cerium oxide	533:563	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	8	2	theme	-CS	1638:1640	arg1	film					1642:1645	the BC-ALG(CeONP)-CS film	1621:1645	the BC-ALG(CeONP)-CS film	1621:1645	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	7	3	theme	mechanical	1448:1457	arg1	strength					1459:1466	a mechanical strength	1446:1466	a mechanical strength at the level of ~2 MPa	1446:1489	In the equilibrium swollen state, the material retains a mechanical strength at the level of ~2 MPa.					
36433128	7	4	theme	swollen	1410:1416	arg1	state					1418:1422	the equilibrium swollen state	1394:1422	the equilibrium swollen state	1394:1422	In the equilibrium swollen state, the material retains a mechanical strength at the level of ~2 MPa.					
36433128	8	5	theme	good	1596:1599	arg1	biocompatibility					1601:1616	the good biocompatibility	1592:1616	the good biocompatibility of the BC-ALG(CeONP)-CS film	1592:1645	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	8	5	theme	good	1596:1599	arg1	scaffolds					1669:1677	cell proliferation scaffolds	1650:1677	cell proliferation scaffolds	1650:1677	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	6	6	dep	oxide	1265:1269	arg1	Ce3+					1323:1326	Ce3+	1323:1326	Ce3+	1323:1326	The latter effect is considered to be caused by the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films.					
36433128	6	6	dep	oxide	1265:1269	arg1	Ce4+					1314:1317	reversible transitions between the states Ce4+	1272:1317	reversible transitions between the states Ce4+	1272:1317	The latter effect is considered to be caused by the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films.					
36433128	4	7	theme	nanoparticles	684:696	arg1	interaction					665:675	the interaction	661:675	the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy,	661:744	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	4	7	theme	nanoparticles	684:696	arg1	presence					599:606	The presence	595:606	The presence of CeONPs in the polysaccharide composite matrix	595:655	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	2	8	theme	natural	295:301	arg1	polysaccharides					303:317	natural polysaccharides	295:317	natural polysaccharides filled with cerium oxide nanoparticles (CeONPs)	295:365	It is based on the composition of natural polysaccharides filled with cerium oxide nanoparticles (CeONPs).					
36433128	8	9	theme	proliferation	1655:1667	arg1	biocompatibility					1601:1616	the good biocompatibility	1592:1616	the good biocompatibility of the BC-ALG(CeONP)-CS film	1592:1645	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	8	9	theme	proliferation	1655:1667	arg1	scaffolds					1669:1677	cell proliferation scaffolds	1650:1677	cell proliferation scaffolds	1650:1677	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	8	10	dep	tests	1516:1520	arg1	cultivation					1523:1533	cultivation	1523:1533	cultivation of multipotent mesenchymal stem cells	1523:1571	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	1	11	theme	biomedical	218:227	arg1	applications					229:240	other biomedical applications	212:240	other biomedical applications	212:240	A new biocompatible nanocomposite film material for cell engineering and other biomedical applications has been prepared.					
36433128	2	12	theme	cerium	331:336	arg1	CeONPs					359:364	CeONPs	359:364	CeONPs	359:364	It is based on the composition of natural polysaccharides filled with cerium oxide nanoparticles (CeONPs).					
36433128	2	12	theme	cerium	331:336	arg1	nanoparticles					344:356	cerium oxide nanoparticles	331:356	cerium oxide nanoparticles (CeONPs)	331:365	It is based on the composition of natural polysaccharides filled with cerium oxide nanoparticles (CeONPs).					
36433128	5	13	theme	material	862:869	arg1	properties					844:853	properties	844:853	properties of the material in comparison with those of the matrix's polysaccharide composition	844:937	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	6	14	theme	transitions	1283:1293	arg1	Ce4+					1314:1317	reversible transitions between the states Ce4+	1272:1317	reversible transitions between the states Ce4+	1272:1317	The latter effect is considered to be caused by the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films.					
36433128	5	15	theme	BC	1033:1034	arg1	macrochains					1036:1046	BC macrochains	1033:1046	BC macrochains	1033:1046	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	16	from	properties	844:853	arg1	comparison					874:883	comparison	874:883	comparison with those of the matrix's polysaccharide composition	874:937	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	1	17	theme	new	141:143	arg1	material					178:185	A new biocompatible nanocomposite film material	139:185	A new biocompatible nanocomposite film material for cell engineering and other biomedical applications	139:240	A new biocompatible nanocomposite film material for cell engineering and other biomedical applications has been prepared.					
36433128	8	18	dep	in	1507:1508	arg1	vitro					1510:1514	vitro	1510:1514	vitro	1510:1514	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	1	19	theme	nanocomposite	159:171	arg1	material					178:185	A new biocompatible nanocomposite film material	139:185	A new biocompatible nanocomposite film material for cell engineering and other biomedical applications	139:240	A new biocompatible nanocomposite film material for cell engineering and other biomedical applications has been prepared.					
36433128	0	20	theme	Cerium	74:79	arg1	Oxide					81:85	Nanosized Cerium Oxide	64:85	Nanosized Cerium Oxide	64:85	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	6	21	from	processes	1353:1361	arg1	films					1384:1388	the nanocomposite films	1366:1388	the nanocomposite films	1366:1388	The latter effect is considered to be caused by the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films.					
36433128	4	22	with	presence	599:606	arg1	polymer					707:713	the polymer	703:713	the polymer	703:713	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	6	23	theme	stimulated	1342:1351	arg1	processes					1353:1361	thermally stimulated processes	1332:1361	thermally stimulated processes in the nanocomposite films	1332:1388	The latter effect is considered to be caused by the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films.					
36433128	1	24	theme	cell	191:194	arg1	engineering					196:206	cell engineering	191:206	cell engineering	191:206	A new biocompatible nanocomposite film material for cell engineering and other biomedical applications has been prepared.					
36433128	4	25	theme	composite	785:793	arg1	architecture					765:776	the network architecture	753:776	the network architecture of the composite	753:793	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	3	26	theme	preparative	372:382	arg1	procedure					384:392	The preparative procedure	368:392	The preparative procedure	368:392	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	4	27	from	presence	599:606	arg1	matrix					650:655	the polysaccharide composite matrix	621:655	the polysaccharide composite matrix	621:655	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	4	28	theme	network	757:763	arg1	architecture					765:776	the network architecture	753:776	the network architecture of the composite	753:793	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	0	29	theme	Bacterial	0:8	arg1	Composites					20:29	Bacterial Cellulose Composites	0:29	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.	0:137	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	0	29	theme	Bacterial	0:8	arg1	Characterization					88:103	Characterization	88:103	Characterization	88:103	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	0	29	theme	Bacterial	0:8	arg1	Assessment					127:136	Cytocompatibility Assessment	109:136	Cytocompatibility Assessment	109:136	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	3	30	theme	successive	406:415	arg1	impregnations					417:429	successive impregnations	406:429	successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution	406:592	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	3	31	theme	ALG	491:493	arg1	solution					496:503	a sodium alginate (ALG) solution	472:503	a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide	472:563	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	5	32	theme	low-temperature	1164:1178	arg1	region					1180:1185	a low-temperature region	1162:1185	a low-temperature region	1162:1185	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	3	33	theme	alginate	481:488	arg1	solution					496:503	a sodium alginate (ALG) solution	472:503	a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide	472:563	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	5	34	theme	destruction	1131:1141	arg1	processes					1101:1109	the processes	1097:1109	the processes of thermo-oxidative destruction of the material to a low-temperature region	1097:1185	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	7	35	theme	MPa	1487:1489	arg1	level					1475:1479	the level	1471:1479	the level of ~2 MPa	1471:1489	In the equilibrium swollen state, the material retains a mechanical strength at the level of ~2 MPa.					
36433128	5	36	theme	planar	1011:1016	arg1	orientation					1018:1028	planar orientation	1011:1028	planar orientation of BC macrochains	1011:1046	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	3	37	theme	bacterial	442:450	arg1	cellulose					452:460	pressed bacterial cellulose	434:460	pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution	434:592	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	3	37	theme	bacterial	442:450	arg1	BC					463:464	BC	463:464	BC	463:464	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	0	38	with	Composites	20:29	arg1	Polysaccharides					36:50	Polysaccharides	36:50	Polysaccharides Filled with Nanosized Cerium Oxide	36:85	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	6	39	theme	nanocomposite	1370:1382	arg1	films					1384:1388	the nanocomposite films	1366:1388	the nanocomposite films	1366:1388	The latter effect is considered to be caused by the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films.					
36433128	4	40	with	interaction	665:675	arg1	polymer					707:713	the polymer	703:713	the polymer	703:713	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	3	41	theme	chitosan	571:578	arg1	solution					585:592	a chitosan (CS) solution	569:592	a chitosan (CS) solution	569:592	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	0	42	dep	Composites	20:29	arg1	Composites					20:29	Bacterial Cellulose Composites	0:29	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.	0:137	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	0	42	dep	Composites	20:29	arg1	Characterization					88:103	Characterization	88:103	Characterization	88:103	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	0	42	dep	Composites	20:29	arg1	Assessment					127:136	Cytocompatibility Assessment	109:136	Cytocompatibility Assessment	109:136	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	5	43	from	decrease	985:992	arg1	stiffness					972:980	mechanical stiffness	961:980	mechanical stiffness	961:980	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	43	from	decrease	985:992	arg1	degree					1001:1006	the degree	997:1006	the degree of planar orientation of BC macrochains	997:1046	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	43	from	decrease	985:992	arg1	hydrophilicity					1064:1077	hydrophilicity	1064:1077	hydrophilicity	1064:1077	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	3	44	theme	oxide	559:563	arg1	nanoparticles					516:528	nanoparticles	516:528	nanoparticles of citrate-stabilized cerium oxide	516:563	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	0	45	theme	Nanosized	64:72	arg1	Oxide					81:85	Nanosized Cerium Oxide	64:85	Nanosized Cerium Oxide	64:85	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	4	46	theme	composite	640:648	arg1	matrix					650:655	the polysaccharide composite matrix	621:655	the polysaccharide composite matrix	621:655	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	6	47	theme	oxide	1265:1269	arg1	activity					1246:1253	the redox activity	1236:1253	the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films	1236:1388	The latter effect is considered to be caused by the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films.					
36433128	3	48	theme	citrate-stabilized	533:550	arg1	oxide					559:563	citrate-stabilized cerium oxide	533:563	citrate-stabilized cerium oxide	533:563	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	5	49	from	changes	821:827	arg1	number					834:839	a number	832:839	a number of properties of the material in comparison with those of the matrix's polysaccharide composition	832:937	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	3	50	with	cellulose	452:460	arg1	solution					585:592	a chitosan (CS) solution	569:592	a chitosan (CS) solution	569:592	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	3	50	with	cellulose	452:460	arg1	solution					496:503	a sodium alginate (ALG) solution	472:503	a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide	472:563	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	4	51	theme	CeONPs	611:616	arg1	interaction					665:675	the interaction	661:675	the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy,	661:744	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	4	51	theme	CeONPs	611:616	arg1	presence					599:606	The presence	595:606	The presence of CeONPs in the polysaccharide composite matrix	595:655	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	6	52	theme	redox	1240:1244	arg1	activity					1246:1253	the redox activity	1236:1253	the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films	1236:1388	The latter effect is considered to be caused by the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films.					
36433128	2	53	theme	polysaccharides	303:317	arg1	composition					280:290	the composition	276:290	the composition of natural polysaccharides filled with cerium oxide nanoparticles (CeONPs)	276:365	It is based on the composition of natural polysaccharides filled with cerium oxide nanoparticles (CeONPs).					
36433128	8	54	theme	film	1642:1645	arg1	biocompatibility					1601:1616	the good biocompatibility	1592:1616	the good biocompatibility of the BC-ALG(CeONP)-CS film	1592:1645	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	8	54	theme	film	1642:1645	arg1	scaffolds					1669:1677	cell proliferation scaffolds	1650:1677	cell proliferation scaffolds	1650:1677	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	7	55	theme	equilibrium	1398:1408	arg1	state					1418:1422	the equilibrium swollen state	1394:1422	the equilibrium swollen state	1394:1422	In the equilibrium swollen state, the material retains a mechanical strength at the level of ~2 MPa.					
36433128	8	56	theme	cell	1650:1653	arg1	biocompatibility					1601:1616	the good biocompatibility	1592:1616	the good biocompatibility of the BC-ALG(CeONP)-CS film	1592:1645	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	8	56	theme	cell	1650:1653	arg1	scaffolds					1669:1677	cell proliferation scaffolds	1650:1677	cell proliferation scaffolds	1650:1677	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	1	57	theme	other	212:216	arg1	applications					229:240	other biomedical applications	212:240	other biomedical applications	212:240	A new biocompatible nanocomposite film material for cell engineering and other biomedical applications has been prepared.					
36433128	2	58	theme	oxide	338:342	arg1	CeONPs					359:364	CeONPs	359:364	CeONPs	359:364	It is based on the composition of natural polysaccharides filled with cerium oxide nanoparticles (CeONPs).					
36433128	2	58	theme	oxide	338:342	arg1	nanoparticles					344:356	cerium oxide nanoparticles	331:356	cerium oxide nanoparticles (CeONPs)	331:365	It is based on the composition of natural polysaccharides filled with cerium oxide nanoparticles (CeONPs).					
36433128	4	59	from	interaction	665:675	arg1	matrix					650:655	the polysaccharide composite matrix	621:655	the polysaccharide composite matrix	621:655	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	5	60	theme	properties	844:853	arg1	number					834:839	a number	832:839	a number of properties of the material in comparison with those of the matrix's polysaccharide composition	832:937	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	61	from	material	862:869	arg1	comparison					874:883	comparison	874:883	comparison with those of the matrix's polysaccharide composition	874:937	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	62	theme	polysaccharide	912:925	arg1	composition					927:937	the matrix's polysaccharide composition	899:937	the matrix's polysaccharide composition	899:937	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	4	63	theme	IR	729:730	arg1	spectroscopy					732:743	IR spectroscopy	729:743	IR spectroscopy	729:743	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	6	64	theme	reversible	1272:1281	arg1	transitions					1283:1293	reversible transitions	1272:1293	reversible transitions between the states Ce4+	1272:1317	The latter effect is considered to be caused by the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films.					
36433128	5	65	theme	macrochains	1036:1046	arg1	orientation					1018:1028	planar orientation	1011:1028	planar orientation of BC macrochains	1011:1046	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	4	66	attach	presence	599:606	arg2	nanoparticles					684:696	the nanoparticles	680:696	the nanoparticles	680:696	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	4	66	attach	presence	599:606	arg1	matrix					650:655	the polysaccharide composite matrix	621:655	the polysaccharide composite matrix	621:655	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	4	66	attach	presence	599:606	arg2	CeONPs					611:616	CeONPs	611:616	CeONPs	611:616	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	1	67	theme	biocompatible	145:157	arg1	material					178:185	A new biocompatible nanocomposite film material	139:185	A new biocompatible nanocomposite film material for cell engineering and other biomedical applications	139:240	A new biocompatible nanocomposite film material for cell engineering and other biomedical applications has been prepared.					
36433128	6	68	theme	latter	1192:1197	arg1	effect					1199:1204	The latter effect	1188:1204	The latter effect	1188:1204	The latter effect is considered to be caused by the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films.					
36433128	1	69	theme	film	173:176	arg1	material					178:185	A new biocompatible nanocomposite film material	139:185	A new biocompatible nanocomposite film material for cell engineering and other biomedical applications	139:240	A new biocompatible nanocomposite film material for cell engineering and other biomedical applications has been prepared.					
36433128	8	70	theme	stem	1562:1565	arg1	cells					1567:1571	multipotent mesenchymal stem cells	1538:1571	multipotent mesenchymal stem cells	1538:1571	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	5	71	theme	processes	1101:1109	arg1	increase					949:956	an increase	946:956	an increase in mechanical stiffness	946:980	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	71	theme	processes	1101:1109	arg1	shift					1088:1092	the shift	1084:1092	the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region	1084:1185	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	71	theme	processes	1101:1109	arg1	decrease					985:992	a decrease	983:992	a decrease in the degree of planar orientation of BC macrochains	983:1046	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	71	theme	processes	1101:1109	arg1	increase					1052:1059	an increase	1049:1059	an increase in hydrophilicity	1049:1077	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	72	from	comparison	874:883	arg1	properties					844:853	properties	844:853	properties of the material in comparison with those of the matrix's polysaccharide composition	844:937	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	0	73	theme	Cytocompatibility	109:125	arg1	Composites					20:29	Bacterial Cellulose Composites	0:29	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.	0:137	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	0	73	theme	Cytocompatibility	109:125	arg1	Assessment					127:136	Cytocompatibility Assessment	109:136	Cytocompatibility Assessment	109:136	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	5	74	theme	thermo-oxidative	1114:1129	arg1	destruction					1131:1141	thermo-oxidative destruction	1114:1141	thermo-oxidative destruction of the material to a low-temperature region	1114:1185	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	75	theme	mechanical	961:970	arg1	stiffness					972:980	mechanical stiffness	961:980	mechanical stiffness	961:980	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	3	76	contain	containing	505:514	arg1	solution					496:503	a sodium alginate (ALG) solution	472:503	a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide	472:563	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	3	76	contain	containing	505:514	arg2	nanoparticles					516:528	nanoparticles	516:528	nanoparticles of citrate-stabilized cerium oxide	516:563	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	5	77	from	increase	1052:1059	arg1	stiffness					972:980	mechanical stiffness	961:980	mechanical stiffness	961:980	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	77	from	increase	1052:1059	arg1	degree					1001:1006	the degree	997:1006	the degree of planar orientation of BC macrochains	997:1046	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	77	from	increase	1052:1059	arg1	hydrophilicity					1064:1077	hydrophilicity	1064:1077	hydrophilicity	1064:1077	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	8	78	theme	mesenchymal	1550:1560	arg1	cells					1567:1571	multipotent mesenchymal stem cells	1538:1571	multipotent mesenchymal stem cells	1538:1571	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	3	79	theme	sodium	474:479	arg1	solution					496:503	a sodium alginate (ALG) solution	472:503	a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide	472:563	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	0	80	theme	Cellulose	10:18	arg1	Composites					20:29	Bacterial Cellulose Composites	0:29	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.	0:137	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	0	80	theme	Cellulose	10:18	arg1	Characterization					88:103	Characterization	88:103	Characterization	88:103	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	0	80	theme	Cellulose	10:18	arg1	Assessment					127:136	Cytocompatibility Assessment	109:136	Cytocompatibility Assessment	109:136	Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment.					
36433128	3	81	theme	pressed	434:440	arg1	cellulose					452:460	pressed bacterial cellulose	434:460	pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution	434:592	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	3	81	theme	pressed	434:440	arg1	BC					463:464	BC	463:464	BC	463:464	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	8	82	theme	in	1507:1508	arg1	tests					1516:1520	in vitro tests	1507:1520	in vitro tests (cultivation of multipotent mesenchymal stem cells)	1507:1572	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	8	83	theme	tests	1516:1520	arg1	results					1496:1502	The results	1492:1502	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells)	1492:1572	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	5	84	theme	noticeable	810:819	arg1	changes					821:827	noticeable changes	810:827	noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition	810:937	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	7	85	from	level	1475:1479	arg1	strength					1459:1466	a mechanical strength	1446:1466	a mechanical strength at the level of ~2 MPa	1446:1489	In the equilibrium swollen state, the material retains a mechanical strength at the level of ~2 MPa.					
36433128	5	86	theme	orientation	1018:1028	arg1	degree					1001:1006	the degree	997:1006	the degree of planar orientation of BC macrochains	997:1046	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	3	87	theme	cellulose	452:460	arg1	impregnations					417:429	successive impregnations	406:429	successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution	406:592	The preparative procedure consists of successive impregnations of pressed bacterial cellulose (BC) with a sodium alginate (ALG) solution containing nanoparticles of citrate-stabilized cerium oxide and a chitosan (CS) solution.					
36433128	8	88	theme	multipotent	1538:1548	arg1	cells					1567:1571	multipotent mesenchymal stem cells	1538:1571	multipotent mesenchymal stem cells	1538:1571	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	5	89	from	increase	949:956	arg1	stiffness					972:980	mechanical stiffness	961:980	mechanical stiffness	961:980	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	89	from	increase	949:956	arg1	degree					1001:1006	the degree	997:1006	the degree of planar orientation of BC macrochains	997:1046	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	89	from	increase	949:956	arg1	hydrophilicity					1064:1077	hydrophilicity	1064:1077	hydrophilicity	1064:1077	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	90	theme	material	1150:1157	arg1	destruction					1131:1141	thermo-oxidative destruction	1114:1141	thermo-oxidative destruction of the material to a low-temperature region	1114:1185	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	4	91	theme	polysaccharide	625:638	arg1	matrix					650:655	the polysaccharide composite matrix	621:655	the polysaccharide composite matrix	621:655	The presence of CeONPs in the polysaccharide composite matrix and the interaction of the nanoparticles with the polymer, confirmed by IR spectroscopy, change the network architecture of the composite.					
36433128	5	92	with	comparison	874:883	arg1	those					890:894	those	890:894	those	890:894	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	8	93	theme	BC-ALG	1625:1630	arg1	film					1642:1645	the BC-ALG(CeONP)-CS film	1621:1645	the BC-ALG(CeONP)-CS film	1621:1645	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	6	94	from	activity	1246:1253	arg1	processes					1353:1361	thermally stimulated processes	1332:1361	thermally stimulated processes in the nanocomposite films	1332:1388	The latter effect is considered to be caused by the redox activity of cerium oxide (reversible transitions between the states Ce4+ and Ce3+) in thermally stimulated processes in the nanocomposite films.					
36433128	8	95	theme	cells	1567:1571	arg1	cultivation					1523:1533	cultivation	1523:1533	cultivation of multipotent mesenchymal stem cells	1523:1571	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
36433128	5	96	from	shift	1088:1092	arg1	stiffness					972:980	mechanical stiffness	961:980	mechanical stiffness	961:980	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	96	from	shift	1088:1092	arg1	degree					1001:1006	the degree	997:1006	the degree of planar orientation of BC macrochains	997:1046	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	5	96	from	shift	1088:1092	arg1	hydrophilicity					1064:1077	hydrophilicity	1064:1077	hydrophilicity	1064:1077	This leads to noticeable changes in a number of properties of the material in comparison with those of the matrix's polysaccharide composition, viz., an increase in mechanical stiffness, a decrease in the degree of planar orientation of BC macrochains, an increase in hydrophilicity, and the shift of the processes of thermo-oxidative destruction of the material to a low-temperature region.					
36433128	8	97	theme	CeONP	1632:1636	arg1	film					1642:1645	the BC-ALG(CeONP)-CS film	1621:1645	the BC-ALG(CeONP)-CS film	1621:1645	The results of in vitro tests (cultivation of multipotent mesenchymal stem cells) have demonstrated the good biocompatibility of the BC-ALG(CeONP)-CS film as cell proliferation scaffolds.					
37307781	8	0	theme	primary	1115:1121	arg1	outcome					1123:1129	the primary outcome	1111:1129	the primary outcome	1111:1129	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	10	1	theme	representative	1630:1643	arg1	markers					1660:1666	the representative liver fibrotic markers	1626:1666	the representative liver fibrotic markers	1626:1666	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
37307781	7	2	theme	follow-up	860:868	arg1	period					870:875	a median follow-up period	851:875	a median follow-up period of 747 (interquartile range 465 to 1,042) days	851:922	During a median follow-up period of 747 (interquartile range 465 to 1,042) days, the primary outcome occurred in 45 patients.					
37307781	7	3	theme	days	919:922	arg1	period					870:875	a median follow-up period	851:875	a median follow-up period of 747 (interquartile range 465 to 1,042) days	851:922	During a median follow-up period of 747 (interquartile range 465 to 1,042) days, the primary outcome occurred in 45 patients.					
37307781	4	4	theme	liver	585:589	arg1	disease					591:597	organic liver disease	577:597	organic liver disease	577:597	We prospectively examined 211 consecutive patients with chronic HF between April 2018 and August 2021, excluding those with organic liver disease, using liver magnetic resonance imaging and ultrasound.					
37307781	7	5	dep	range	899:903	arg1	to					909:910	to	909:910	to	909:910	During a median follow-up period of 747 (interquartile range 465 to 1,042) days, the primary outcome occurred in 45 patients.					
37307781	7	5	dep	range	899:903	arg1	1,042					912:916	1,042	912:916	interquartile range 465 to 1,042	885:916	During a median follow-up period of 747 (interquartile range 465 to 1,042) days, the primary outcome occurred in 45 patients.					
37307781	9	6	theme	primary	1588:1594	arg1	outcome					1596:1602	the primary outcome	1584:1602	the primary outcome	1584:1602	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	9	7	theme	hazard	1404:1409	arg1	ratio					1411:1415	hazard ratio 2.89	1404:1420	hazard ratio 2.89	1404:1420	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	2	8	theme	optimal	186:192	arg1	markers					194:200	the optimal markers	182:200	the optimal markers for outcome prediction	182:223	However, the optimal markers for outcome prediction remain unclear.					
37307781	8	9	dep	showed	1068:1073	arg1	p					1151:1151	p <0.001	1151:1158	p <0.001	1151:1158	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	8	9	dep	showed	1068:1073	arg1	p = 0.005					1164:1172	p = 0.005	1164:1172	p = 0.005	1164:1172	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	5	10	theme	markers	698:704	arg1	total					657:661	A total	655:661	A total of 7 representative liver fibrotic markers	655:704	A total of 7 representative liver fibrotic markers were measured in all patients.					
37307781	9	11	theme	multivariable	1194:1206	arg1	analysis					1223:1230	The multivariable Cox regression analysis	1190:1230	The multivariable Cox regression analysis	1190:1230	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	9	12	theme	regression	1212:1221	arg1	analysis					1223:1230	The multivariable Cox regression analysis	1190:1230	The multivariable Cox regression analysis	1190:1230	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	10	13	theme	optimal	1710:1716	arg1	acid					1680:1683	hyaluronic acid	1669:1683	hyaluronic acid	1669:1683	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
37307781	10	13	theme	optimal	1710:1716	arg1	markers					1718:1724	the optimal markers	1706:1724	the optimal markers for outcome prediction in patients with HF	1706:1767	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
37307781	10	13	theme	optimal	1710:1716	arg1	P-III-P					1689:1695	P-III-P	1689:1695	P-III-P	1689:1695	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
37307781	10	14	with	patients	1752:1759	arg1	HF					1766:1767	HF	1766:1767	HF	1766:1767	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
37307781	9	15	theme	mortality	1503:1511	arg1	model					1524:1528	a mortality prediction model	1501:1528	a mortality prediction model	1501:1528	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	5	16	theme	liver	683:687	arg1	markers					698:704	7 representative liver fibrotic markers	666:704	7 representative liver fibrotic markers	666:704	A total of 7 representative liver fibrotic markers were measured in all patients.					
37307781	3	17	theme	liver	312:316	arg1	markers					327:333	liver fibrotic markers	312:333	liver fibrotic markers	312:333	This study aimed to simultaneously investigate the prognostic value of liver fibrotic markers and the associations between these markers and clinical parameters in patients with HF without organic liver disease.					
37307781	9	18	theme	hyaluronic	1246:1255	arg1	acid					1257:1260	hyaluronic acid	1246:1260	hyaluronic acid	1246:1260	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	2	19	theme	outcome	206:212	arg1	prediction					214:223	outcome prediction	206:223	outcome prediction	206:223	However, the optimal markers for outcome prediction remain unclear.					
37307781	8	20	theme	hyaluronic	991:1000	arg1	acid					1002:1005	higher hyaluronic acid	984:1005	higher hyaluronic acid	984:1005	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	7	21	theme	primary	929:935	arg1	outcome					937:943	the primary outcome	925:943	the primary outcome	925:943	During a median follow-up period of 747 (interquartile range 465 to 1,042) days, the primary outcome occurred in 45 patients.					
37307781	1	22	theme	heart	153:157	arg1	HF					168:169	HF	168:169	HF	168:169	Several liver fibrotic markers are associated with prognosis in patients with heart failure (HF).					
37307781	1	22	theme	heart	153:157	arg1	failure					159:165	heart failure	153:165	heart failure (HF)	153:170	Several liver fibrotic markers are associated with prognosis in patients with heart failure (HF).					
37307781	4	23	theme	chronic	509:515	arg1	HF					517:518	chronic HF	509:518	chronic HF	509:518	We prospectively examined 211 consecutive patients with chronic HF between April 2018 and August 2021, excluding those with organic liver disease, using liver magnetic resonance imaging and ultrasound.					
37307781	6	24	theme	all-cause	790:798	arg1	death					800:804	all-cause death	790:804	all-cause death	790:804	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	9	25	dep	interval	1438:1445	arg1	ratio					1351:1355	hazard ratio 1.84	1344:1360	hazard ratio 1.84	1344:1360	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	1	26	with	patients	139:146	arg1	HF					168:169	HF	168:169	HF	168:169	Several liver fibrotic markers are associated with prognosis in patients with heart failure (HF).					
37307781	1	26	with	patients	139:146	arg1	failure					159:165	heart failure	153:165	heart failure (HF)	153:170	Several liver fibrotic markers are associated with prognosis in patients with heart failure (HF).					
37307781	10	27	from	markers	1718:1724	arg1	conclusion					1608:1617	conclusion	1608:1617	conclusion	1608:1617	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
37307781	8	28	with	Patients	970:977	arg1	type					1011:1014	type III	1011:1018	type III	1011:1018	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	8	28	with	Patients	970:977	arg1	acid					1002:1005	higher hyaluronic acid	984:1005	higher hyaluronic acid	984:1005	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	3	29	from	markers	370:376	arg1	patients					405:412	patients	405:412	patients with HF without organic liver disease	405:450	This study aimed to simultaneously investigate the prognostic value of liver fibrotic markers and the associations between these markers and clinical parameters in patients with HF without organic liver disease.					
37307781	0	30	theme	Prognostic	0:9	arg1	Value					11:15	Prognostic Value	0:15	Prognostic Value of Liver Fibrotic Markers in Patients With Heart Failure	0:72	Prognostic Value of Liver Fibrotic Markers in Patients With Heart Failure.					
37307781	8	31	theme	N-terminal	1032:1041	arg1	P-III-P					1052:1058	P-III-P	1052:1058	P-III-P	1052:1058	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	8	31	theme	N-terminal	1032:1041	arg1	peptide					1043:1049	procollagen N-terminal peptide	1020:1049	procollagen N-terminal peptide (P-III-P) levels	1020:1066	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	1	32	theme	liver	83:87	arg1	markers					98:104	Several liver fibrotic markers	75:104	Several liver fibrotic markers	75:104	Several liver fibrotic markers are associated with prognosis in patients with heart failure (HF).					
37307781	9	33	theme	prediction	1513:1522	arg1	model					1524:1528	a mortality prediction model	1501:1528	a mortality prediction model	1501:1528	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	3	34	theme	liver	438:442	arg1	disease					444:450	organic liver disease	430:450	organic liver disease	430:450	This study aimed to simultaneously investigate the prognostic value of liver fibrotic markers and the associations between these markers and clinical parameters in patients with HF without organic liver disease.					
37307781	0	35	theme	Fibrotic	26:33	arg1	Markers					35:41	Liver Fibrotic Markers	20:41	Liver Fibrotic Markers	20:41	Prognostic Value of Liver Fibrotic Markers in Patients With Heart Failure.					
37307781	9	36	theme	adverse	1328:1334	arg1	events					1336:1341	adverse events	1328:1341	adverse events	1328:1341	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	3	37	theme	clinical	382:389	arg1	parameters					391:400	clinical parameters	382:400	clinical parameters in patients with HF without organic liver disease	382:450	This study aimed to simultaneously investigate the prognostic value of liver fibrotic markers and the associations between these markers and clinical parameters in patients with HF without organic liver disease.					
37307781	7	38	theme	interquartile	885:897	arg1	days					919:922	747 (interquartile range 465 to 1,042) days	880:922	747 (interquartile range 465 to 1,042) days	880:922	During a median follow-up period of 747 (interquartile range 465 to 1,042) days, the primary outcome occurred in 45 patients.					
37307781	7	38	theme	interquartile	885:897	arg1	range					899:903	interquartile range 465 to 1,042	885:916	interquartile range 465 to 1,042	885:916	During a median follow-up period of 747 (interquartile range 465 to 1,042) days, the primary outcome occurred in 45 patients.					
37307781	10	39	theme	liver	1645:1649	arg1	markers					1660:1666	the representative liver fibrotic markers	1626:1666	the representative liver fibrotic markers	1626:1666	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
37307781	9	40	theme	acid	1257:1260	arg1	levels					1274:1279	hyaluronic acid and P-III-P levels	1246:1279	hyaluronic acid and P-III-P levels	1246:1279	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	6	41	theme	death	800:804	arg1	death					800:804	all-cause death	790:804	all-cause death	790:804	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	6	41	theme	death	800:804	arg1	hospitalization					810:824	hospitalization	810:824	hospitalization	810:824	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	6	41	theme	death	800:804	arg1	composite					777:785	the composite	773:785	the composite of all-cause death and hospitalization for worsening HF	773:841	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	6	41	theme	death	800:804	arg1	outcome					749:755	The primary outcome	737:755	The primary outcome of interest	737:767	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	8	42	theme	outcome	1123:1129	arg1	incidence					1098:1106	a significantly higher incidence	1075:1106	a significantly higher incidence of the primary outcome	1075:1129	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	4	43	theme	organic	577:583	arg1	disease					591:597	organic liver disease	577:597	organic liver disease	577:597	We prospectively examined 211 consecutive patients with chronic HF between April 2018 and August 2021, excluding those with organic liver disease, using liver magnetic resonance imaging and ultrasound.					
37307781	8	44	dep	acid	1002:1005	arg1	levels					1061:1066	procollagen N-terminal peptide (P-III-P) levels	1020:1066	procollagen N-terminal peptide (P-III-P) levels	1020:1066	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	10	45	theme	hyaluronic	1669:1678	arg1	acid					1680:1683	hyaluronic acid	1669:1683	hyaluronic acid	1669:1683	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
37307781	10	45	theme	hyaluronic	1669:1678	arg1	markers					1718:1724	the optimal markers	1706:1724	the optimal markers for outcome prediction in patients with HF	1706:1767	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
37307781	10	45	theme	hyaluronic	1669:1678	arg1	P-III-P					1689:1695	P-III-P	1689:1695	P-III-P	1689:1695	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
37307781	7	46	theme	median	853:858	arg1	period					870:875	a median follow-up period	851:875	a median follow-up period of 747 (interquartile range 465 to 1,042) days	851:922	During a median follow-up period of 747 (interquartile range 465 to 1,042) days, the primary outcome occurred in 45 patients.					
37307781	3	47	with	patients	405:412	arg1	HF					419:420	HF	419:420	HF	419:420	This study aimed to simultaneously investigate the prognostic value of liver fibrotic markers and the associations between these markers and clinical parameters in patients with HF without organic liver disease.					
37307781	4	48	theme	ultrasound	643:652	arg1	resonance					621:629	liver magnetic resonance	606:629	liver magnetic resonance imaging and ultrasound	606:652	We prospectively examined 211 consecutive patients with chronic HF between April 2018 and August 2021, excluding those with organic liver disease, using liver magnetic resonance imaging and ultrasound.					
37307781	8	49	theme	higher	984:989	arg1	acid					1002:1005	higher hyaluronic acid	984:1005	higher hyaluronic acid	984:1005	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	5	50	theme	fibrotic	689:696	arg1	markers					698:704	7 representative liver fibrotic markers	666:704	7 representative liver fibrotic markers	666:704	A total of 7 representative liver fibrotic markers were measured in all patients.					
37307781	6	51	theme	interest	760:767	arg1	death					800:804	all-cause death	790:804	all-cause death	790:804	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	6	51	theme	interest	760:767	arg1	hospitalization					810:824	hospitalization	810:824	hospitalization	810:824	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	6	51	theme	interest	760:767	arg1	composite					777:785	the composite	773:785	the composite of all-cause death and hospitalization for worsening HF	773:841	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	6	51	theme	interest	760:767	arg1	outcome					749:755	The primary outcome	737:755	The primary outcome of interest	737:767	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	4	52	theme	magnetic	612:619	arg1	resonance					621:629	liver magnetic resonance	606:629	liver magnetic resonance imaging and ultrasound	606:652	We prospectively examined 211 consecutive patients with chronic HF between April 2018 and August 2021, excluding those with organic liver disease, using liver magnetic resonance imaging and ultrasound.					
37307781	10	53	theme	outcome	1730:1736	arg1	prediction					1738:1747	outcome prediction	1730:1747	outcome prediction in patients with HF	1730:1767	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
37307781	4	54	theme	imaging	631:637	arg1	resonance					621:629	liver magnetic resonance	606:629	liver magnetic resonance imaging and ultrasound	606:652	We prospectively examined 211 consecutive patients with chronic HF between April 2018 and August 2021, excluding those with organic liver disease, using liver magnetic resonance imaging and ultrasound.					
37307781	9	55	theme	Cox	1208:1210	arg1	analysis					1223:1230	The multivariable Cox regression analysis	1190:1230	The multivariable Cox regression analysis	1190:1230	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	3	56	theme	prognostic	292:301	arg1	value					303:307	the prognostic value	288:307	the prognostic value of liver fibrotic markers	288:333	This study aimed to simultaneously investigate the prognostic value of liver fibrotic markers and the associations between these markers and clinical parameters in patients with HF without organic liver disease.					
37307781	5	57	theme	representative	668:681	arg1	markers					698:704	7 representative liver fibrotic markers	666:704	7 representative liver fibrotic markers	666:704	A total of 7 representative liver fibrotic markers were measured in all patients.					
37307781	0	58	theme	Heart	60:64	arg1	Failure					66:72	Heart Failure	60:72	Heart Failure	60:72	Prognostic Value of Liver Fibrotic Markers in Patients With Heart Failure.					
37307781	3	59	theme	fibrotic	318:325	arg1	markers					327:333	liver fibrotic markers	312:333	liver fibrotic markers	312:333	This study aimed to simultaneously investigate the prognostic value of liver fibrotic markers and the associations between these markers and clinical parameters in patients with HF without organic liver disease.					
37307781	3	60	from	parameters	391:400	arg1	patients					405:412	patients	405:412	patients with HF without organic liver disease	405:450	This study aimed to simultaneously investigate the prognostic value of liver fibrotic markers and the associations between these markers and clinical parameters in patients with HF without organic liver disease.					
37307781	0	61	from	Value	11:15	arg1	Patients					46:53	Patients	46:53	Patients With Heart Failure	46:72	Prognostic Value of Liver Fibrotic Markers in Patients With Heart Failure.					
37307781	9	62	theme	P-III-P	1266:1272	arg1	levels					1274:1279	hyaluronic acid and P-III-P levels	1246:1279	hyaluronic acid and P-III-P levels	1246:1279	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	8	63	theme	procollagen	1020:1030	arg1	P-III-P					1052:1058	P-III-P	1052:1058	P-III-P	1052:1058	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	8	63	theme	procollagen	1020:1030	arg1	peptide					1043:1049	procollagen N-terminal peptide	1020:1049	procollagen N-terminal peptide (P-III-P) levels	1020:1066	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	4	64	theme	consecutive	483:493	arg1	patients					495:502	211 consecutive patients	479:502	211 consecutive patients with chronic HF between April 2018 and August 2021, excluding those with organic liver disease	479:597	We prospectively examined 211 consecutive patients with chronic HF between April 2018 and August 2021, excluding those with organic liver disease, using liver magnetic resonance imaging and ultrasound.					
37307781	3	65	theme	markers	327:333	arg1	value					303:307	the prognostic value	288:307	the prognostic value of liver fibrotic markers	288:333	This study aimed to simultaneously investigate the prognostic value of liver fibrotic markers and the associations between these markers and clinical parameters in patients with HF without organic liver disease.					
37307781	3	65	theme	markers	327:333	arg1	associations					343:354	the associations	339:354	the associations between these markers and clinical parameters in patients with HF without organic liver disease	339:450	This study aimed to simultaneously investigate the prognostic value of liver fibrotic markers and the associations between these markers and clinical parameters in patients with HF without organic liver disease.					
37307781	8	66	theme	peptide	1043:1049	arg1	levels					1061:1066	procollagen N-terminal peptide (P-III-P) levels	1020:1066	procollagen N-terminal peptide (P-III-P) levels	1020:1066	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	1	67	from	prognosis	126:134	arg1	patients					139:146	patients	139:146	patients with heart failure (HF)	139:170	Several liver fibrotic markers are associated with prognosis in patients with heart failure (HF).					
37307781	1	68	theme	Several	75:81	arg1	markers					98:104	Several liver fibrotic markers	75:104	Several liver fibrotic markers	75:104	Several liver fibrotic markers are associated with prognosis in patients with heart failure (HF).					
37307781	0	69	theme	Liver	20:24	arg1	Markers					35:41	Liver Fibrotic Markers	20:41	Liver Fibrotic Markers	20:41	Prognostic Value of Liver Fibrotic Markers in Patients With Heart Failure.					
37307781	1	70	theme	fibrotic	89:96	arg1	markers					98:104	Several liver fibrotic markers	75:104	Several liver fibrotic markers	75:104	Several liver fibrotic markers are associated with prognosis in patients with heart failure (HF).					
37307781	10	71	from	prediction	1738:1747	arg1	patients					1752:1759	patients	1752:1759	patients with HF	1752:1767	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
37307781	9	72	dep	6.34	1455:1458	arg1	to					1452:1453	to	1452:1453	to	1452:1453	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	0	73	theme	Markers	35:41	arg1	Value					11:15	Prognostic Value	0:15	Prognostic Value of Liver Fibrotic Markers in Patients With Heart Failure	0:72	Prognostic Value of Liver Fibrotic Markers in Patients With Heart Failure.					
37307781	9	74	theme	events	1336:1341	arg1	risk					1320:1323	the risk	1316:1323	the risk of adverse events	1316:1341	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	9	75	dep	2.87	1395:1398	arg1	to					1392:1393	to	1392:1393	to	1392:1393	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	6	76	theme	primary	741:747	arg1	death					800:804	all-cause death	790:804	all-cause death	790:804	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	6	76	theme	primary	741:747	arg1	hospitalization					810:824	hospitalization	810:824	hospitalization	810:824	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	6	76	theme	primary	741:747	arg1	composite					777:785	the composite	773:785	the composite of all-cause death and hospitalization for worsening HF	773:841	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	6	76	theme	primary	741:747	arg1	outcome					749:755	The primary outcome	737:755	The primary outcome of interest	737:767	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	9	77	theme	hazard	1344:1349	arg1	ratio					1351:1355	hazard ratio 1.84	1344:1360	hazard ratio 1.84	1344:1360	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	8	78	theme	higher	1091:1096	arg1	incidence					1098:1106	a significantly higher incidence	1075:1106	a significantly higher incidence of the primary outcome	1075:1129	Patients with higher hyaluronic acid and type III procollagen N-terminal peptide (P-III-P) levels showed a significantly higher incidence of the primary outcome than those without (p <0.001 and p = 0.005, respectively).					
37307781	6	79	theme	worsening	830:838	arg1	HF					840:841	worsening HF	830:841	worsening HF	830:841	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	9	80	theme	other	1543:1547	arg1	markers					1551:1557	the other 5 markers	1539:1557	the other 5 markers	1539:1557	The multivariable Cox regression analysis revealed that hyaluronic acid and P-III-P levels were independently associated with the risk of adverse events (hazard ratio 1.84, 95% confidence interval 1.18 to 2.87 and hazard ratio 2.89, 95% confidence interval 1.32 to 6.34, respectively) even after adjustment for a mortality prediction model, whereas the other 5 markers were not associated with the primary outcome.					
37307781	0	81	with	Patients	46:53	arg1	Failure					66:72	Heart Failure	60:72	Heart Failure	60:72	Prognostic Value of Liver Fibrotic Markers in Patients With Heart Failure.					
37307781	4	82	with	patients	495:502	arg1	HF					517:518	chronic HF	509:518	chronic HF	509:518	We prospectively examined 211 consecutive patients with chronic HF between April 2018 and August 2021, excluding those with organic liver disease, using liver magnetic resonance imaging and ultrasound.					
37307781	6	83	theme	hospitalization	810:824	arg1	death					800:804	all-cause death	790:804	all-cause death	790:804	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	6	83	theme	hospitalization	810:824	arg1	hospitalization					810:824	hospitalization	810:824	hospitalization	810:824	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	6	83	theme	hospitalization	810:824	arg1	composite					777:785	the composite	773:785	the composite of all-cause death and hospitalization for worsening HF	773:841	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	6	83	theme	hospitalization	810:824	arg1	outcome					749:755	The primary outcome	737:755	The primary outcome of interest	737:767	The primary outcome of interest was the composite of all-cause death and hospitalization for worsening HF.					
37307781	3	84	theme	organic	430:436	arg1	disease					444:450	organic liver disease	430:450	organic liver disease	430:450	This study aimed to simultaneously investigate the prognostic value of liver fibrotic markers and the associations between these markers and clinical parameters in patients with HF without organic liver disease.					
37307781	4	85	theme	liver	606:610	arg1	resonance					621:629	liver magnetic resonance	606:629	liver magnetic resonance imaging and ultrasound	606:652	We prospectively examined 211 consecutive patients with chronic HF between April 2018 and August 2021, excluding those with organic liver disease, using liver magnetic resonance imaging and ultrasound.					
37307781	10	86	theme	fibrotic	1651:1658	arg1	markers					1660:1666	the representative liver fibrotic markers	1626:1666	the representative liver fibrotic markers	1626:1666	In conclusion, among the representative liver fibrotic markers, hyaluronic acid and P-III-P might be the optimal markers for outcome prediction in patients with HF.					
36754263	3	0	theme	%	666:666	arg1	increase					668:675	>189 % increase	661:675	>189 % increase	661:675	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	3	1	dep	blocking	651:658	arg1	increase					668:675	>189 % increase	661:675	>189 % increase	661:675	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	3	1	dep	blocking	651:658	arg1	UV					648:649	UV	648:649	UV	648:649	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	7	2	theme	Active	1388:1393	arg1	materials					1507:1515	multifunctional packaging materials	1481:1515	multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods	1481:1604	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	2	theme	Active	1388:1393	arg1	films					1411:1415	48 h. Active and intelligent films	1382:1415	48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA	1382:1449	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	3	3	theme	fabricated	696:705	arg1	films					707:711	the fabricated films	692:711	the fabricated films	692:711	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	1	4	theme	real-time	285:293	arg1	potentials					303:312	real-time sensing potentials	285:312	real-time sensing potentials	285:312	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	7	5	theme	shrimp	1272:1277	arg1	freshness					1236:1244	the freshness	1232:1244	the freshness of minced pork, fish, and shrimp	1232:1277	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	1	6	theme	carbon	192:197	arg1	films					242:246	carbon dot-based active and intelligent packaging films	192:246	carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials	192:312	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	3	7	theme	BO-CD	579:583	arg1	incorporation					556:568	The incorporation	552:568	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix	552:613	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	1	8	theme	active	209:214	arg1	films					242:246	carbon dot-based active and intelligent packaging films	192:246	carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials	192:312	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	0	9	theme	intelligent	131:141	arg1	applications					158:169	active and intelligent food packaging applications	120:169	active and intelligent food packaging applications	120:169	Cellulose nanofiber-based multifunctional films integrated with carbon dots and anthocyanins from Brassica oleracea for active and intelligent food packaging applications.					
36754263	3	10	theme	1.5 	573:576	arg1	%					577:577	%	577:577	%	577:577	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	1	11	theme	intelligent	220:230	arg1	films					242:246	carbon dot-based active and intelligent packaging films	192:246	carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials	192:312	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	0	12	theme	packaging	148:156	arg1	applications					158:169	active and intelligent food packaging applications	120:169	active and intelligent food packaging applications	120:169	Cellulose nanofiber-based multifunctional films integrated with carbon dots and anthocyanins from Brassica oleracea for active and intelligent food packaging applications.					
36754263	3	13	theme	BOA	593:595	arg1	incorporation					556:568	The incorporation	552:568	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix	552:613	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	5	14	theme	radical	837:843	arg1	activity					856:863	strong radical scavenging activity	830:863	strong radical scavenging activity against ABTS (~90 %) and DPPH (~80 %)	830:901	It showed strong radical scavenging activity against ABTS (~90 %) and DPPH (~80 %) compared to the neat CNF film.					
36754263	7	15	theme	perishable	1589:1598	arg1	foods					1600:1604	packaged perishable foods	1580:1604	packaged perishable foods	1580:1604	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	3	16	theme	6 	589:590	arg1	%					591:591	%	591:591	%	591:591	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	7	17	theme	visual	1311:1316	arg1	change					1318:1323	a distinct visual change	1300:1323	a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods	1300:1604	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	18	theme	shelf	1566:1570	arg1	life					1572:1575	the shelf life	1562:1575	the shelf life of packaged perishable foods	1562:1604	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	2	19	theme	BO-biowaste-derived	510:528	arg1	dots					537:540	BO-biowaste-derived carbon dots	510:540	BO-biowaste-derived carbon dots (BO-CDs)	510:549	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	2	19	theme	BO-biowaste-derived	510:528	arg1	BO-CDs					543:548	BO-CDs	543:548	BO-CDs	543:548	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	0	20	from	oleracea	107:114	arg1	anthocyanins					80:91	anthocyanins	80:91	anthocyanins from Brassica oleracea for active and intelligent food packaging applications	80:169	Cellulose nanofiber-based multifunctional films integrated with carbon dots and anthocyanins from Brassica oleracea for active and intelligent food packaging applications.					
36754263	0	20	from	oleracea	107:114	arg1	dots					71:74	carbon dots	64:74	carbon dots	64:74	Cellulose nanofiber-based multifunctional films integrated with carbon dots and anthocyanins from Brassica oleracea for active and intelligent food packaging applications.					
36754263	2	21	used	used	401:404	arg2	CNF					392:394	CNF	392:394	CNF	392:394	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	2	21	used	used	401:404	arg2	nanofiber					381:389	The cellulose nanofiber	367:389	The cellulose nanofiber (CNF)	367:395	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	6	22	theme	electron	943:950	arg1	microscopy					952:961	Scanning electron microscopy	934:961	Scanning electron microscopy	934:961	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	4	23	theme	high	745:748	arg1	fluorescence					750:761	high fluorescence	745:761	high fluorescence	745:761	The synthesized BO-CD exhibits high fluorescence, UV absorption, antibacterial and antioxidant functions.					
36754263	3	24	from	incorporation	556:568	arg1	matrix					608:613	the CNF matrix	600:613	the CNF matrix	600:613	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	5	25	theme	neat	919:922	arg1	film					928:931	the neat CNF film	915:931	the neat CNF film	915:931	It showed strong radical scavenging activity against ABTS (~90 %) and DPPH (~80 %) compared to the neat CNF film.					
36754263	0	26	theme	active	120:125	arg1	applications					158:169	active and intelligent food packaging applications	120:169	active and intelligent food packaging applications	120:169	Cellulose nanofiber-based multifunctional films integrated with carbon dots and anthocyanins from Brassica oleracea for active and intelligent food packaging applications.					
36754263	6	27	theme	photoelectron	973:985	arg1	spectroscopy					987:998	X-ray photoelectron spectroscopy	967:998	X-ray photoelectron spectroscopy	967:998	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	0	28	theme	Cellulose	0:8	arg1	films					42:46	Cellulose nanofiber-based multifunctional films	0:46	Cellulose nanofiber-based multifunctional films	0:46	Cellulose nanofiber-based multifunctional films integrated with carbon dots and anthocyanins from Brassica oleracea for active and intelligent food packaging applications.					
36754263	1	29	theme	sensing	295:301	arg1	potentials					303:312	real-time sensing potentials	285:312	real-time sensing potentials	285:312	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	2	30	theme	nanocomposite	449:461	arg1	film					463:466	the multifunctional intelligent nanocomposite film	417:466	the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs)	417:549	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	2	31	theme	multifunctional	421:435	arg1	film					463:466	the multifunctional intelligent nanocomposite film	417:466	the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs)	417:549	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	0	32	theme	multifunctional	26:40	arg1	films					42:46	Cellulose nanofiber-based multifunctional films	0:46	Cellulose nanofiber-based multifunctional films	0:46	Cellulose nanofiber-based multifunctional films integrated with carbon dots and anthocyanins from Brassica oleracea for active and intelligent food packaging applications.					
36754263	1	33	with	films	242:246	arg1	potentials					303:312	real-time sensing potentials	285:312	real-time sensing potentials	285:312	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	1	33	with	films	242:246	arg1	antibacterial					266:278	antibacterial	266:278	antibacterial	266:278	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	1	33	with	films	242:246	arg1	UV					253:254	UV blocking	253:263	UV blocking	253:263	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	4	34	theme	antioxidant	797:807	arg1	functions					809:817	antioxidant functions	797:817	antioxidant functions	797:817	The synthesized BO-CD exhibits high fluorescence, UV absorption, antibacterial and antioxidant functions.					
36754263	7	35	theme	Packaging	1121:1129	arg1	tests					1131:1135	Packaging tests	1121:1135	Packaging tests	1121:1135	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	36	theme	multifunctional	1481:1495	arg1	materials					1507:1515	multifunctional packaging materials	1481:1515	multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods	1481:1604	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	36	theme	multifunctional	1481:1495	arg1	films					1411:1415	48 h. Active and intelligent films	1382:1415	48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA	1382:1449	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	37	theme	fish	1262:1265	arg1	freshness					1236:1244	the freshness	1232:1244	the freshness of minced pork, fish, and shrimp	1232:1277	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	2	38	theme	BO	484:485	arg1	BOA					501:503	BOA	501:503	BOA	501:503	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	2	38	theme	BO	484:485	arg1	anthocyanins					487:498	BO anthocyanins	484:498	BO anthocyanins (BOA)	484:504	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	0	39	theme	carbon	64:69	arg1	dots					71:74	carbon dots	64:74	carbon dots	64:74	Cellulose nanofiber-based multifunctional films integrated with carbon dots and anthocyanins from Brassica oleracea for active and intelligent food packaging applications.					
36754263	1	40	theme	oleracea	344:351	arg1	extract					358:364	Brassica oleracea (BO) extract	335:364	Brassica oleracea (BO) extract	335:364	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	7	41	theme	pork	1256:1259	arg1	freshness					1236:1244	the freshness	1232:1244	the freshness of minced pork, fish, and shrimp	1232:1277	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	6	42	theme	BO-CDs/BOA	1073:1082	arg1	additives					1084:1092	the BO-CDs/BOA additives	1069:1092	the BO-CDs/BOA additives in the CNF-polymer matrix	1069:1118	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	3	43	theme	blocking	651:658	arg1	properties					678:687	the physicochemical and UV blocking (>189 % increase) properties	624:687	the physicochemical and UV blocking (>189 % increase) properties of the fabricated films	624:711	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	1	44	theme	blocking	256:263	arg1	UV					253:254	UV blocking	253:263	UV blocking	253:263	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	2	45	theme	cellulose	371:379	arg1	CNF					392:394	CNF	392:394	CNF	392:394	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	2	45	theme	cellulose	371:379	arg1	nanofiber					381:389	The cellulose nanofiber	367:389	The cellulose nanofiber (CNF)	367:395	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	6	46	theme	elemental	1044:1052	arg1	composition					1054:1064	elemental composition	1044:1064	elemental composition	1044:1064	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	3	47	theme	films	707:711	arg1	properties					678:687	the physicochemical and UV blocking (>189 % increase) properties	624:687	the physicochemical and UV blocking (>189 % increase) properties of the fabricated films	624:711	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	6	48	from	compatibility	1026:1038	arg1	matrix					1113:1118	the CNF-polymer matrix	1097:1118	the CNF-polymer matrix	1097:1118	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	5	49	theme	scavenging	845:854	arg1	activity					856:863	strong radical scavenging activity	830:863	strong radical scavenging activity against ABTS (~90 %) and DPPH (~80 %)	830:901	It showed strong radical scavenging activity against ABTS (~90 %) and DPPH (~80 %) compared to the neat CNF film.					
36754263	7	50	theme	intelligent	1399:1409	arg1	materials					1507:1515	multifunctional packaging materials	1481:1515	multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods	1481:1604	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	50	theme	intelligent	1399:1409	arg1	films					1411:1415	48 h. Active and intelligent films	1382:1415	48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA	1382:1449	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	51	theme	quality	1535:1541	arg1	changes					1543:1549	quality changes	1535:1549	quality changes	1535:1549	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	52	theme	48 h.	1382:1386	arg1	materials					1507:1515	multifunctional packaging materials	1481:1515	multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods	1481:1604	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	52	theme	48 h.	1382:1386	arg1	films					1411:1415	48 h. Active and intelligent films	1382:1415	48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA	1382:1449	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	53	dep	developed	1417:1425	arg1	based					1427:1431	based	1427:1431	developed based on CNF/BO-CDs/BOA	1417:1449	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	3	54	theme	%	577:577	arg1	BO-CD					579:583	1.5 % BO-CD	573:583	1.5 % BO-CD	573:583	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	1	55	theme	dot-based	199:207	arg1	films					242:246	carbon dot-based active and intelligent packaging films	192:246	carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials	192:312	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	0	56	theme	food	143:146	arg1	applications					158:169	active and intelligent food packaging applications	120:169	active and intelligent food packaging applications	120:169	Cellulose nanofiber-based multifunctional films integrated with carbon dots and anthocyanins from Brassica oleracea for active and intelligent food packaging applications.					
36754263	7	57	theme	foods	1600:1604	arg1	life					1572:1575	the shelf life	1562:1575	the shelf life of packaged perishable foods	1562:1604	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	6	58	theme	CNF-polymer	1101:1111	arg1	matrix					1113:1118	the CNF-polymer matrix	1097:1118	the CNF-polymer matrix	1097:1118	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	3	59	theme	%	591:591	arg1	BOA					593:595	6 % BOA	589:595	6 % BOA	589:595	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	5	60	theme	strong	830:835	arg1	activity					856:863	strong radical scavenging activity	830:863	strong radical scavenging activity against ABTS (~90 %) and DPPH (~80 %)	830:901	It showed strong radical scavenging activity against ABTS (~90 %) and DPPH (~80 %) compared to the neat CNF film.					
36754263	7	61	theme	packaged	1580:1587	arg1	foods					1600:1604	packaged perishable foods	1580:1604	packaged perishable foods	1580:1604	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	2	62	link	BO-biowaste-derived	510:528	arg1	dots					537:540	BO-biowaste-derived carbon dots	510:540	BO-biowaste-derived carbon dots (BO-CDs)	510:549	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	2	62	link	BO-biowaste-derived	510:528	arg1	BO-CDs					543:548	BO-CDs	543:548	BO-CDs	543:548	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	3	63	theme	CNF	604:606	arg1	matrix					608:613	the CNF matrix	600:613	the CNF matrix	600:613	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	6	64	theme	spectroscopy	987:998	arg1	XPS					1001:1003	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS)	934:1004	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS)	934:1004	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	7	65	from	25 °C	1372:1376	arg1	storage					1361:1367	storage	1361:1367	storage at 25 °C	1361:1376	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	6	66	theme	X-ray	967:971	arg1	spectroscopy					987:998	X-ray photoelectron spectroscopy	967:998	X-ray photoelectron spectroscopy	967:998	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	7	67	theme	distinct	1302:1309	arg1	change					1318:1323	a distinct visual change	1300:1323	a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods	1300:1604	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	0	68	theme	Brassica	98:105	arg1	oleracea					107:114	Brassica oleracea	98:114	Brassica oleracea	98:114	Cellulose nanofiber-based multifunctional films integrated with carbon dots and anthocyanins from Brassica oleracea for active and intelligent food packaging applications.					
36754263	1	69	theme	new	174:176	arg1	generation					178:187	A new generation	172:187	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials	172:312	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	6	70	theme	microscopy	952:961	arg1	XPS					1001:1003	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS)	934:1004	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS)	934:1004	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	6	71	theme	Scanning	934:941	arg1	microscopy					952:961	Scanning electron microscopy	934:961	Scanning electron microscopy	934:961	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	7	72	theme	prepared	1153:1160	arg1	able					1216:1219	able	1216:1219	able	1216:1219	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	72	theme	prepared	1153:1160	arg1	film					1162:1165	the prepared film	1149:1165	the prepared film	1149:1165	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	5	73	theme	CNF	924:926	arg1	film					928:931	the neat CNF film	915:931	the neat CNF film	915:931	It showed strong radical scavenging activity against ABTS (~90 %) and DPPH (~80 %) compared to the neat CNF film.					
36754263	2	74	theme	carbon	530:535	arg1	dots					537:540	BO-biowaste-derived carbon dots	510:540	BO-biowaste-derived carbon dots (BO-CDs)	510:549	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	2	74	theme	carbon	530:535	arg1	BO-CDs					543:548	BO-CDs	543:548	BO-CDs	543:548	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	0	75	theme	nanofiber-based	10:24	arg1	films					42:46	Cellulose nanofiber-based multifunctional films	0:46	Cellulose nanofiber-based multifunctional films	0:46	Cellulose nanofiber-based multifunctional films integrated with carbon dots and anthocyanins from Brassica oleracea for active and intelligent food packaging applications.					
36754263	6	76	theme	enhanced	1017:1024	arg1	compatibility					1026:1038	enhanced compatibility	1017:1038	enhanced compatibility	1017:1038	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	6	77	from	matrix	1113:1118	arg1	compatibility					1026:1038	enhanced compatibility	1017:1038	enhanced compatibility	1017:1038	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	6	77	from	matrix	1113:1118	arg1	composition					1054:1064	elemental composition	1044:1064	elemental composition	1044:1064	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	7	78	from	red	1330:1332	arg1	change					1318:1323	a distinct visual change	1300:1323	a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods	1300:1604	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	2	79	theme	intelligent	437:447	arg1	film					463:466	the multifunctional intelligent nanocomposite film	417:466	the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs)	417:549	The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs).					
36754263	3	80	theme	physicochemical	628:642	arg1	properties					678:687	the physicochemical and UV blocking (>189 % increase) properties	624:687	the physicochemical and UV blocking (>189 % increase) properties of the fabricated films	624:711	The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films.					
36754263	4	81	theme	synthesized	718:728	arg1	BO-CD					730:734	The synthesized BO-CD	714:734	The synthesized BO-CD	714:734	The synthesized BO-CD exhibits high fluorescence, UV absorption, antibacterial and antioxidant functions.					
36754263	1	82	theme	packaging	232:240	arg1	films					242:246	carbon dot-based active and intelligent packaging films	192:246	carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials	192:312	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	6	83	from	additives	1084:1092	arg1	matrix					1113:1118	the CNF-polymer matrix	1097:1118	the CNF-polymer matrix	1097:1118	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	1	84	theme	Brassica	335:342	arg1	BO					354:355	BO	354:355	BO	354:355	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	1	84	theme	Brassica	335:342	arg1	oleracea					344:351	Brassica oleracea	335:351	Brassica oleracea (BO) extract	335:364	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	7	85	theme	packaging	1497:1505	arg1	materials					1507:1515	multifunctional packaging materials	1481:1515	multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods	1481:1604	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	85	theme	packaging	1497:1505	arg1	films					1411:1415	48 h. Active and intelligent films	1382:1415	48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA	1382:1449	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	7	86	theme	minced	1249:1254	arg1	pork					1256:1259	minced pork	1249:1259	minced pork	1249:1259	Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.					
36754263	6	87	theme	additives	1084:1092	arg1	compatibility					1026:1038	enhanced compatibility	1017:1038	enhanced compatibility	1017:1038	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	6	87	theme	additives	1084:1092	arg1	composition					1054:1064	elemental composition	1044:1064	elemental composition	1044:1064	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	4	88	theme	UV	764:765	arg1	absorption					767:776	UV absorption	764:776	UV absorption	764:776	The synthesized BO-CD exhibits high fluorescence, UV absorption, antibacterial and antioxidant functions.					
36754263	6	89	from	composition	1054:1064	arg1	matrix					1113:1118	the CNF-polymer matrix	1097:1118	the CNF-polymer matrix	1097:1118	Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix.					
36754263	1	90	theme	films	242:246	arg1	generation					178:187	A new generation	172:187	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials	172:312	A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract.					
36754263	5	91	dep	showed	823:828	arg1	compared					903:910	compared	903:910	showed strong radical scavenging activity against ABTS (~90 %) and DPPH (~80 %) compared to the neat CNF film	823:931	It showed strong radical scavenging activity against ABTS (~90 %) and DPPH (~80 %) compared to the neat CNF film.					
36430612	6	0	theme	L929	992:995	arg1	fibroblasts					1004:1014	L929 murine fibroblasts	992:1014	L929 murine fibroblasts	992:1014	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	6	1	with	studies	884:890	arg1	fibroblasts					1004:1014	L929 murine fibroblasts	992:1014	L929 murine fibroblasts	992:1014	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	7	2	from	profiles	1044:1051	arg1	hydrogels					1070:1078	hydrogels	1070:1078	hydrogels	1070:1078	The release profiles of proteins from hydrogels were also verified.					
36430612	6	3	theme	reduction	971:979	arg1	assay					981:985	MTT reduction assay	967:985	MTT reduction assay	967:985	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	14	4	theme	murine	1799:1804	arg1	proliferation					1817:1829	murine fibroblast proliferation	1799:1829	murine fibroblast proliferation	1799:1829	92.73% for albumin-modified hydrogel, and such an increase indicated the positive impact of the albumin on murine fibroblast proliferation.					
36430612	8	5	theme	increased	1165:1173	arg1	flexibility					1175:1185	increased flexibility	1165:1185	increased flexibility even by 50%	1165:1197	Materials modified with proteins showed higher swelling ability, increased flexibility even by 50% and increased surface hydrophilicity.					
36430612	6	6	theme	incubation	873:882	arg1	studies					884:890	incubation studies	873:890	incubation studies in simulated physiological liquids	873:925	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	11	7	theme	effective	1409:1417	arg1	release					1419:1425	the effective release	1405:1425	the effective release of protein particles from hydrogels in acidic environment (approximately 70%)	1405:1503	Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined.					
36430612	4	8	theme	diacrylate	612:621	arg1	photoinitiator					710:723	a photoinitiator	708:723	a photoinitiator	708:723	Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator.					
36430612	4	8	theme	diacrylate	612:621	arg1	poly					623:626	diacrylate poly	612:626	diacrylate poly(ethylene glycol)	612:643	Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator.					
36430612	4	8	theme	diacrylate	612:621	arg1	glycol					637:642	ethylene glycol	628:642	ethylene glycol	628:642	Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator.					
36430612	4	8	theme	diacrylate	612:621	arg1	agent					663:667	a crosslinking agent	648:667	a crosslinking agent	648:667	Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator.					
36430612	5	9	theme	mechanical	765:774	arg1	properties					776:785	mechanical properties	765:785	mechanical properties	765:785	Next, the hydrogels' swelling ability, mechanical properties, wettability and surface morphology were characterized.					
36430612	4	10	theme	ethylene	628:635	arg1	poly					623:626	diacrylate poly	612:626	diacrylate poly(ethylene glycol)	612:643	Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator.					
36430612	4	10	theme	ethylene	628:635	arg1	glycol					637:642	ethylene glycol	628:642	ethylene glycol	628:642	Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator.					
36430612	6	11	from	analysis	954:961	arg1	liquids					919:925	simulated physiological liquids	895:925	simulated physiological liquids	895:925	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	4	12	theme	Such	505:508	arg1	systems					510:516	Such systems	505:516	Such systems	505:516	Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator.					
36430612	10	13	dep	MPa	1368:1370	arg1	0.11					1363:1366	0.11	1363:1366	0.11	1363:1366	0.11 MPa.					
36430612	8	14	theme	surface	1213:1219	arg1	hydrophilicity					1221:1234	increased surface hydrophilicity	1203:1234	increased surface hydrophilicity	1203:1234	Materials modified with proteins showed higher swelling ability, increased flexibility even by 50% and increased surface hydrophilicity.					
36430612	6	15	theme	MTT	967:969	arg1	assay					981:985	MTT reduction assay	967:985	MTT reduction assay	967:985	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	8	16	theme	swelling	1147:1154	arg1	ability					1156:1162	higher swelling ability	1140:1162	higher swelling ability	1140:1162	Materials modified with proteins showed higher swelling ability, increased flexibility even by 50% and increased surface hydrophilicity.					
36430612	4	17	theme	crosslinking	650:661	arg1	agent					663:667	a crosslinking agent	648:667	a crosslinking agent	648:667	Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator.					
36430612	4	17	theme	crosslinking	650:661	arg1	poly					623:626	diacrylate poly	612:626	diacrylate poly(ethylene glycol)	612:643	Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator.					
36430612	1	18	theme	research	89:96	arg1	hydrogels					146:154	natural polymer-based hydrogels	124:154	natural polymer-based hydrogels modified with albumin particles	124:186	The research subject of this paper are natural polymer-based hydrogels modified with albumin particles.					
36430612	1	18	theme	research	89:96	arg1	subject					98:104	The research subject	85:104	The research subject of this paper	85:118	The research subject of this paper are natural polymer-based hydrogels modified with albumin particles.					
36430612	6	19	from	spectroscopy	859:870	arg1	liquids					919:925	simulated physiological liquids	895:925	simulated physiological liquids	895:925	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	3	20	theme	favorable	373:381	arg1	composition					383:393	The most favorable composition	364:393	The most favorable composition showing monodispersity and particles with a size lower than 40 nm	364:459	The most favorable composition showing monodispersity and particles with a size lower than 40 nm was selected for modification of hydrogels.					
36430612	14	21	theme	albumin-modified	1703:1718	arg1	hydrogel					1720:1727	albumin-modified hydrogel	1703:1727	albumin-modified hydrogel	1703:1727	92.73% for albumin-modified hydrogel, and such an increase indicated the positive impact of the albumin on murine fibroblast proliferation.					
36430612	8	22	theme	higher	1140:1145	arg1	ability					1156:1162	higher swelling ability	1140:1162	higher swelling ability	1140:1162	Materials modified with proteins showed higher swelling ability, increased flexibility even by 50% and increased surface hydrophilicity.					
36430612	14	23	theme	positive	1765:1772	arg1	impact					1774:1779	the positive impact	1761:1779	the positive impact of the albumin on murine fibroblast proliferation	1761:1829	92.73% for albumin-modified hydrogel, and such an increase indicated the positive impact of the albumin on murine fibroblast proliferation.					
36430612	6	24	with	spectroscopy	859:870	arg1	fibroblasts					1004:1014	L929 murine fibroblasts	992:1014	L929 murine fibroblasts	992:1014	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	11	25	from	possibility	1390:1400	arg1	hydrogels					1453:1461	hydrogels	1453:1461	hydrogels in acidic environment (approximately 70%)	1453:1503	Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined.					
36430612	12	26	theme	Incubation	1521:1530	arg1	studies					1532:1538	Incubation studies	1521:1538	Incubation studies	1521:1538	Incubation studies showed hydrogels' stability and lack of their degradation in tested media.					
36430612	7	27	from	hydrogels	1070:1078	arg1	proteins					1056:1063	proteins	1056:1063	proteins from hydrogels	1056:1078	The release profiles of proteins from hydrogels were also verified.					
36430612	7	27	from	hydrogels	1070:1078	arg1	profiles					1044:1051	The release profiles	1032:1051	The release profiles of proteins from hydrogels	1032:1078	The release profiles of proteins from hydrogels were also verified.					
36430612	11	28	theme	particles	1438:1446	arg1	release					1419:1425	the effective release	1405:1425	the effective release of protein particles from hydrogels in acidic environment (approximately 70%)	1405:1503	Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined.					
36430612	4	29	theme	UV	593:594	arg1	radiation					596:604	UV radiation	593:604	UV radiation	593:604	Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator.					
36430612	9	30	theme	tensile	1302:1308	arg1	strength					1310:1317	the tensile strength	1298:1317	the tensile strength of albumin-containing hydrogels	1298:1349	Hydrogels' contact angles were within the range 62-69° while the tensile strength of albumin-containing hydrogels was approx.					
36430612	6	31	theme	physiological	905:917	arg1	liquids					919:925	simulated physiological liquids	895:925	simulated physiological liquids	895:925	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	14	32	theme	fibroblast	1806:1815	arg1	proliferation					1817:1829	murine fibroblast proliferation	1799:1829	murine fibroblast proliferation	1799:1829	92.73% for albumin-modified hydrogel, and such an increase indicated the positive impact of the albumin on murine fibroblast proliferation.					
36430612	9	33	theme	albumin-containing	1322:1339	arg1	hydrogels					1341:1349	albumin-containing hydrogels	1322:1349	albumin-containing hydrogels	1322:1349	Hydrogels' contact angles were within the range 62-69° while the tensile strength of albumin-containing hydrogels was approx.					
36430612	0	34	theme	Hydrogels	38:46	arg1	Development					0:10	Development	0:10	Development of Chitosan/Gelatin-Based Hydrogels	0:46	Development of Chitosan/Gelatin-Based Hydrogels Incorporated with Albumin Particles.					
36430612	6	35	theme	FT-IR	853:857	arg1	spectroscopy					859:870	FT-IR spectroscopy	853:870	FT-IR spectroscopy	853:870	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	2	36	theme	UV-Vis	320:325	arg1	spectroscopy					327:338	UV-Vis spectroscopy	320:338	UV-Vis spectroscopy	320:338	The proteins were obtained via the salt-induced precipitation method, and next characterized using dynamic light scattering (DLS), UV-Vis spectroscopy and FT-IR spectroscopy.					
36430612	14	37	theme	albumin	1788:1794	arg1	impact					1774:1779	the positive impact	1761:1779	the positive impact of the albumin on murine fibroblast proliferation	1761:1829	92.73% for albumin-modified hydrogel, and such an increase indicated the positive impact of the albumin on murine fibroblast proliferation.					
36430612	0	38	theme	Chitosan/Gelatin-Based	15:36	arg1	Hydrogels					38:46	Chitosan/Gelatin-Based Hydrogels	15:46	Chitosan/Gelatin-Based Hydrogels	15:46	Development of Chitosan/Gelatin-Based Hydrogels Incorporated with Albumin Particles.					
36430612	5	39	theme	swelling	747:754	arg1	ability					756:762	the hydrogels' swelling ability	732:762	the hydrogels' swelling ability	732:762	Next, the hydrogels' swelling ability, mechanical properties, wettability and surface morphology were characterized.					
36430612	6	40	theme	activity	945:952	arg1	analysis					954:961	pro-inflammatory activity analysis	928:961	pro-inflammatory activity analysis	928:961	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	7	41	theme	release	1036:1042	arg1	profiles					1044:1051	The release profiles	1032:1051	The release profiles of proteins from hydrogels	1032:1078	The release profiles of proteins from hydrogels were also verified.					
36430612	3	42	theme	hydrogels	494:502	arg1	modification					478:489	modification	478:489	modification of hydrogels	478:502	The most favorable composition showing monodispersity and particles with a size lower than 40 nm was selected for modification of hydrogels.					
36430612	3	43	theme	lower	444:448	arg1	size					439:442	a size	437:442	a size lower than 40 nm	437:459	The most favorable composition showing monodispersity and particles with a size lower than 40 nm was selected for modification of hydrogels.					
36430612	4	44	theme	radiation	596:604	arg1	influence					580:588	the influence	576:588	the influence of UV radiation	576:604	Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator.					
36430612	12	45	theme	tested	1601:1606	arg1	media					1608:1612	tested media	1601:1612	tested media	1601:1612	Incubation studies showed hydrogels' stability and lack of their degradation in tested media.					
36430612	6	46	theme	pro-inflammatory	928:943	arg1	analysis					954:961	pro-inflammatory activity analysis	928:961	pro-inflammatory activity analysis	928:961	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	6	47	from	studies	884:890	arg1	liquids					919:925	simulated physiological liquids	895:925	simulated physiological liquids	895:925	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	11	48	theme	acidic	1466:1471	arg1	environment					1473:1483	acidic environment	1466:1483	acidic environment (approximately 70%)	1466:1503	Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined.					
36430612	11	48	theme	acidic	1466:1471	arg1	%					1502:1502	approximately 70%	1486:1502	approximately 70%	1486:1502	Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined.					
36430612	9	49	theme	hydrogels	1341:1349	arg1	strength					1310:1317	the tensile strength	1298:1317	the tensile strength of albumin-containing hydrogels	1298:1349	Hydrogels' contact angles were within the range 62-69° while the tensile strength of albumin-containing hydrogels was approx.					
36430612	11	50	theme	release	1419:1425	arg1	possibility					1390:1400	the possibility	1386:1400	the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%)	1386:1503	Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined.					
36430612	6	51	with	analysis	954:961	arg1	fibroblasts					1004:1014	L929 murine fibroblasts	992:1014	L929 murine fibroblasts	992:1014	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	6	52	from	assay	981:985	arg1	liquids					919:925	simulated physiological liquids	895:925	simulated physiological liquids	895:925	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	14	53	from	impact	1774:1779	arg1	proliferation					1817:1829	murine fibroblast proliferation	1799:1829	murine fibroblast proliferation	1799:1829	92.73% for albumin-modified hydrogel, and such an increase indicated the positive impact of the albumin on murine fibroblast proliferation.					
36430612	1	54	theme	natural	124:130	arg1	hydrogels					146:154	natural polymer-based hydrogels	124:154	natural polymer-based hydrogels modified with albumin particles	124:186	The research subject of this paper are natural polymer-based hydrogels modified with albumin particles.					
36430612	1	54	theme	natural	124:130	arg1	subject					98:104	The research subject	85:104	The research subject of this paper	85:118	The research subject of this paper are natural polymer-based hydrogels modified with albumin particles.					
36430612	0	55	theme	Albumin	66:72	arg1	Particles					74:82	Albumin Particles	66:82	Albumin Particles	66:82	Development of Chitosan/Gelatin-Based Hydrogels Incorporated with Albumin Particles.					
36430612	2	56	theme	precipitation	237:249	arg1	method					251:256	the salt-induced precipitation method	220:256	the salt-induced precipitation method	220:256	The proteins were obtained via the salt-induced precipitation method, and next characterized using dynamic light scattering (DLS), UV-Vis spectroscopy and FT-IR spectroscopy.					
36430612	6	57	theme	simulated	895:903	arg1	liquids					919:925	simulated physiological liquids	895:925	simulated physiological liquids	895:925	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	11	58	theme	protein	1430:1436	arg1	particles					1438:1446	protein particles	1430:1446	protein particles	1430:1446	Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined.					
36430612	1	59	theme	polymer-based	132:144	arg1	hydrogels					146:154	natural polymer-based hydrogels	124:154	natural polymer-based hydrogels modified with albumin particles	124:186	The research subject of this paper are natural polymer-based hydrogels modified with albumin particles.					
36430612	1	59	theme	polymer-based	132:144	arg1	subject					98:104	The research subject	85:104	The research subject of this paper	85:118	The research subject of this paper are natural polymer-based hydrogels modified with albumin particles.					
36430612	13	60	theme	unmodified	1659:1668	arg1	hydrogel					1670:1677	unmodified hydrogel	1659:1677	unmodified hydrogel	1659:1677	The viability of fibroblasts was 89.54% for unmodified hydrogel, and approx.					
36430612	8	61	theme	increased	1203:1211	arg1	hydrophilicity					1221:1234	increased surface hydrophilicity	1203:1234	increased surface hydrophilicity	1203:1234	Materials modified with proteins showed higher swelling ability, increased flexibility even by 50% and increased surface hydrophilicity.					
36430612	2	62	theme	salt-induced	224:235	arg1	method					251:256	the salt-induced precipitation method	220:256	the salt-induced precipitation method	220:256	The proteins were obtained via the salt-induced precipitation method, and next characterized using dynamic light scattering (DLS), UV-Vis spectroscopy and FT-IR spectroscopy.					
36430612	2	63	theme	light	296:300	arg1	DLS					314:316	DLS	314:316	DLS	314:316	The proteins were obtained via the salt-induced precipitation method, and next characterized using dynamic light scattering (DLS), UV-Vis spectroscopy and FT-IR spectroscopy.					
36430612	2	63	theme	light	296:300	arg1	scattering					302:311	dynamic light scattering	288:311	dynamic light scattering (DLS)	288:317	The proteins were obtained via the salt-induced precipitation method, and next characterized using dynamic light scattering (DLS), UV-Vis spectroscopy and FT-IR spectroscopy.					
36430612	1	64	theme	paper	114:118	arg1	hydrogels					146:154	natural polymer-based hydrogels	124:154	natural polymer-based hydrogels modified with albumin particles	124:186	The research subject of this paper are natural polymer-based hydrogels modified with albumin particles.					
36430612	1	64	theme	paper	114:118	arg1	subject					98:104	The research subject	85:104	The research subject of this paper	85:118	The research subject of this paper are natural polymer-based hydrogels modified with albumin particles.					
36430612	6	65	with	assay	981:985	arg1	fibroblasts					1004:1014	L929 murine fibroblasts	992:1014	L929 murine fibroblasts	992:1014	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	12	66	theme	degradation	1586:1596	arg1	lack					1572:1575	lack	1572:1575	lack of their degradation	1572:1596	Incubation studies showed hydrogels' stability and lack of their degradation in tested media.					
36430612	12	66	theme	degradation	1586:1596	arg1	stability					1558:1566	hydrogels' stability	1547:1566	hydrogels' stability	1547:1566	Incubation studies showed hydrogels' stability and lack of their degradation in tested media.					
36430612	2	67	theme	dynamic	288:294	arg1	DLS					314:316	DLS	314:316	DLS	314:316	The proteins were obtained via the salt-induced precipitation method, and next characterized using dynamic light scattering (DLS), UV-Vis spectroscopy and FT-IR spectroscopy.					
36430612	2	67	theme	dynamic	288:294	arg1	scattering					302:311	dynamic light scattering	288:311	dynamic light scattering (DLS)	288:317	The proteins were obtained via the salt-induced precipitation method, and next characterized using dynamic light scattering (DLS), UV-Vis spectroscopy and FT-IR spectroscopy.					
36430612	2	68	theme	FT-IR	344:348	arg1	spectroscopy					350:361	FT-IR spectroscopy	344:361	FT-IR spectroscopy	344:361	The proteins were obtained via the salt-induced precipitation method, and next characterized using dynamic light scattering (DLS), UV-Vis spectroscopy and FT-IR spectroscopy.					
36430612	9	69	theme	contact	1248:1254	arg1	angles					1256:1261	Hydrogels' contact angles	1237:1261	Hydrogels' contact angles	1237:1261	Hydrogels' contact angles were within the range 62-69° while the tensile strength of albumin-containing hydrogels was approx.					
36430612	11	70	from	hydrogels	1453:1461	arg1	%					1502:1502	approximately 70%	1486:1502	approximately 70%	1486:1502	Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined.					
36430612	11	70	from	hydrogels	1453:1461	arg1	release					1419:1425	the effective release	1405:1425	the effective release of protein particles from hydrogels in acidic environment (approximately 70%)	1405:1503	Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined.					
36430612	11	70	from	hydrogels	1453:1461	arg1	environment					1473:1483	acidic environment	1466:1483	acidic environment (approximately 70%)	1466:1503	Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined.					
36430612	11	70	from	hydrogels	1453:1461	arg1	possibility					1390:1400	the possibility	1386:1400	the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%)	1386:1503	Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined.					
36430612	5	71	theme	surface	804:810	arg1	morphology					812:821	surface morphology	804:821	surface morphology	804:821	Next, the hydrogels' swelling ability, mechanical properties, wettability and surface morphology were characterized.					
36430612	6	72	theme	murine	997:1002	arg1	fibroblasts					1004:1014	L929 murine fibroblasts	992:1014	L929 murine fibroblasts	992:1014	Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed.					
36430612	13	73	theme	fibroblasts	1632:1642	arg1	%					1653:1653	89.54%	1648:1653	89.54%	1648:1653	The viability of fibroblasts was 89.54% for unmodified hydrogel, and approx.					
36430612	13	73	theme	fibroblasts	1632:1642	arg1	viability					1619:1627	The viability	1615:1627	The viability of fibroblasts	1615:1642	The viability of fibroblasts was 89.54% for unmodified hydrogel, and approx.					
36430612	7	74	theme	proteins	1056:1063	arg1	profiles					1044:1051	The release profiles	1032:1051	The release profiles of proteins from hydrogels	1032:1078	The release profiles of proteins from hydrogels were also verified.					
36430612	1	75	theme	albumin	170:176	arg1	particles					178:186	albumin particles	170:186	albumin particles	170:186	The research subject of this paper are natural polymer-based hydrogels modified with albumin particles.					
35408695	8	0	theme	@	1601:1601	arg1	pectin					1602:1607	the composite AC-RH@pectin	1582:1607	the composite AC-RH@pectin	1582:1607	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	1	1	theme	enterosorbents-materials	145:168	arg1	use					138:140	The use	134:140	The use of enterosorbents-materials which can be administered orally and eliminate toxic substances from the gastrointestinal tract (GIT) by sorption-offers an attractive complementary protection of humans against acute and chronic poisoning.	134:375	The use of enterosorbents-materials which can be administered orally and eliminate toxic substances from the gastrointestinal tract (GIT) by sorption-offers an attractive complementary protection of humans against acute and chronic poisoning.					
35408695	7	2	theme	Langmuir	1176:1183	arg1	adsorption					1185:1194	the Langmuir adsorption	1172:1194	the Langmuir adsorption isotherm model	1172:1209	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	1	3	theme	chronic	358:364	arg1	poisoning					366:374	acute and chronic poisoning	348:374	acute and chronic poisoning	348:374	The use of enterosorbents-materials which can be administered orally and eliminate toxic substances from the gastrointestinal tract (GIT) by sorption-offers an attractive complementary protection of humans against acute and chronic poisoning.					
35408695	4	4	theme	nitrate	821:827	arg1	removal					800:806	the removal	796:806	the removal of lead (II) nitrate	796:827	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	0	5	from	Intoxication	74:85	arg1	Protection					58:67	Human Protection	52:67	Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac	52:131	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	9	6	theme	composite	1693:1701	arg1	pectin					1718:1723	the composite material AC-RH@pectin	1689:1723	the composite material AC-RH@pectin	1689:1723	Therefore, the composite material AC-RH@pectin demonstrated substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature.					
35408695	5	7	theme	high	974:977	arg1	capacity					990:997	high adsorption capacity	974:997	high adsorption capacity for both adsorbates studied	974:1025	The composite enterosorbent demonstrated high adsorption capacity for both adsorbates studied.					
35408695	9	8	theme	universal	1825:1833	arg1	enterosorbent					1835:1847	a more universal enterosorbent	1818:1847	a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature	1818:1929	Therefore, the composite material AC-RH@pectin demonstrated substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature.					
35408695	9	8	theme	universal	1825:1833	arg1	it					1812:1813	it	1812:1813	it	1812:1813	Therefore, the composite material AC-RH@pectin demonstrated substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature.					
35408695	4	9	theme	synthesized	736:746	arg1	materials					748:756	the synthesized materials	732:756	the synthesized materials	732:756	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	6	10	theme	AC-RH	1104:1108	arg1	pectin					1110:1115	AC-RH@pectin	1104:1115	AC-RH@pectin	1104:1115	Adsorption kinetics of lead and diclofenac adsorption by AC-RH, pectin, and AC-RH@pectin, fitted well a pseudo-second-order model.					
35408695	8	11	theme	maximum	1491:1497	arg1	capacity					1510:1517	the maximum adsorption capacity	1487:1517	the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g,	1487:1552	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	8	11	theme	maximum	1491:1497	arg1	lower					1563:1567	lower	1563:1567	lower	1563:1567	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	4	12	theme	adsorption	707:716	arg1	properties					718:727	The adsorption properties	703:727	The adsorption properties of the synthesized materials	703:756	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	4	12	theme	adsorption	707:716	arg1	representative					834:847	a representative	832:847	a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug	832:930	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	3	13	theme	low	555:557	arg1	degree					559:564	low degree	555:564	low degree of esterification	555:582	It was designed with a core-shell structure based on pectin with low degree of esterification as the core, and nanoporous activated carbon produced from rice husk, AC-RH, as the shell, designated as AC-RH@pectin.					
35408695	9	14	theme	AC-RH	1712:1716	arg1	pectin					1718:1723	the composite material AC-RH@pectin	1689:1723	the composite material AC-RH@pectin	1689:1723	Therefore, the composite material AC-RH@pectin demonstrated substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature.					
35408695	9	15	theme	suitable	1849:1856	arg1	enterosorbent					1835:1847	a more universal enterosorbent	1818:1847	a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature	1818:1929	Therefore, the composite material AC-RH@pectin demonstrated substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature.					
35408695	9	15	theme	suitable	1849:1856	arg1	it					1812:1813	it	1812:1813	it	1812:1813	Therefore, the composite material AC-RH@pectin demonstrated substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature.					
35408695	0	16	with	Intoxication	74:85	arg1	Xenobiotics					92:102	Xenobiotics Pb(II) and Sodium Diclofenac	92:131	Xenobiotics Pb(II) and Sodium Diclofenac	92:131	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	0	16	with	Intoxication	74:85	arg1	Pb					104:105	Pb	104:105	Pb(II)	104:109	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	0	16	with	Intoxication	74:85	arg1	Diclofenac					122:131	Sodium Diclofenac	115:131	Sodium Diclofenac	115:131	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	9	17	theme	@	1717:1717	arg1	pectin					1718:1723	the composite material AC-RH@pectin	1689:1723	the composite material AC-RH@pectin	1689:1723	Therefore, the composite material AC-RH@pectin demonstrated substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature.					
35408695	8	18	theme	adsorption	1617:1626	arg1	capacity					1628:1635	the adsorption capacity	1613:1635	the adsorption capacity of pectin for diclofenac	1613:1660	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	8	18	theme	adsorption	1617:1626	arg1	negligible					1666:1675	negligible	1666:1675	negligible	1666:1675	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	3	19	theme	AC-RH	689:693	arg1	pectin					695:700	AC-RH@pectin	689:700	AC-RH@pectin	689:700	It was designed with a core-shell structure based on pectin with low degree of esterification as the core, and nanoporous activated carbon produced from rice husk, AC-RH, as the shell, designated as AC-RH@pectin.					
35408695	4	20	theme	lead	811:814	arg1	nitrate					821:827	lead (II) nitrate	811:827	lead (II) nitrate	811:827	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	8	21	theme	pectin	1640:1645	arg1	capacity					1628:1635	the adsorption capacity	1613:1635	the adsorption capacity of pectin for diclofenac	1613:1660	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	8	21	theme	pectin	1640:1645	arg1	negligible					1666:1675	negligible	1666:1675	negligible	1666:1675	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	4	22	theme	materials	748:756	arg1	properties					718:727	The adsorption properties	703:727	The adsorption properties of the synthesized materials	703:756	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	4	22	theme	materials	748:756	arg1	representative					834:847	a representative	832:847	a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug	832:930	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	6	23	theme	diclofenac	1060:1069	arg1	adsorption					1071:1080	diclofenac adsorption	1060:1080	diclofenac adsorption by AC-RH, pectin, and AC-RH@pectin,	1060:1116	Adsorption kinetics of lead and diclofenac adsorption by AC-RH, pectin, and AC-RH@pectin, fitted well a pseudo-second-order model.					
35408695	6	24	theme	lead	1051:1054	arg1	adsorption					1071:1080	diclofenac adsorption	1060:1080	diclofenac adsorption by AC-RH, pectin, and AC-RH@pectin,	1060:1116	Adsorption kinetics of lead and diclofenac adsorption by AC-RH, pectin, and AC-RH@pectin, fitted well a pseudo-second-order model.					
35408695	6	24	theme	lead	1051:1054	arg1	kinetics					1039:1046	Adsorption kinetics	1028:1046	Adsorption kinetics of lead	1028:1054	Adsorption kinetics of lead and diclofenac adsorption by AC-RH, pectin, and AC-RH@pectin, fitted well a pseudo-second-order model.					
35408695	0	25	theme	Carbon/Pectin	10:22	arg1	Enterosorbent					34:46	Carbon/Pectin Composite Enterosorbent	10:46	Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac	10:131	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	3	26	theme	@	694:694	arg1	pectin					695:700	AC-RH@pectin	689:700	AC-RH@pectin	689:700	It was designed with a core-shell structure based on pectin with low degree of esterification as the core, and nanoporous activated carbon produced from rice husk, AC-RH, as the shell, designated as AC-RH@pectin.					
35408695	7	27	theme	adsorption	1185:1194	arg1	model					1205:1209	the Langmuir adsorption isotherm model	1172:1209	the Langmuir adsorption isotherm model	1172:1209	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	27	theme	adsorption	1185:1194	arg1	model					1237:1241	the best fitted isotherm model	1212:1241	the best fitted isotherm model	1212:1241	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	3	28	theme	esterification	569:582	arg1	degree					559:564	low degree	555:564	low degree of esterification	555:582	It was designed with a core-shell structure based on pectin with low degree of esterification as the core, and nanoporous activated carbon produced from rice husk, AC-RH, as the shell, designated as AC-RH@pectin.					
35408695	8	29	theme	52.7	1543:1546	arg1	lead					1532:1535	lead (II)	1532:1540	lead (II)	1532:1540	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	8	29	theme	52.7	1543:1546	arg1	mg/g					1548:1551	52.7 mg/g	1543:1551	52.7 mg/g	1543:1551	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	2	30	theme	biomedical	453:462	arg1	preparation					464:474	a microgranulated binary biomedical preparation	428:474	a microgranulated binary biomedical preparation for oral use	428:487	In this study, we report the results of developing a microgranulated binary biomedical preparation for oral use.					
35408695	8	31	theme	pectin	1440:1445	arg1	qmax					1386:1389	qmax	1386:1389	qmax of AC-RH for diclofenac	1386:1413	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	8	31	theme	pectin	1440:1445	arg1	mg/g					1422:1425	537.6 mg/g	1416:1425	537.6 mg/g	1416:1425	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	8	31	theme	pectin	1440:1445	arg1	qmax					1432:1435	qmax	1432:1435	qmax	1432:1435	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	0	32	dep	Xenobiotics	92:102	arg1	Xenobiotics					92:102	Xenobiotics Pb(II) and Sodium Diclofenac	92:131	Xenobiotics Pb(II) and Sodium Diclofenac	92:131	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	0	32	dep	Xenobiotics	92:102	arg1	II					107:108	II	107:108	II	107:108	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	0	32	dep	Xenobiotics	92:102	arg1	Pb					104:105	Pb	104:105	Pb(II)	104:109	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	0	32	dep	Xenobiotics	92:102	arg1	Diclofenac					122:131	Sodium Diclofenac	115:131	Sodium Diclofenac	115:131	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	9	33	theme	substantial	1738:1748	arg1	efficiency					1750:1759	substantial efficiency	1738:1759	substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature	1738:1929	Therefore, the composite material AC-RH@pectin demonstrated substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature.					
35408695	2	34	theme	binary	446:451	arg1	preparation					464:474	a microgranulated binary biomedical preparation	428:474	a microgranulated binary biomedical preparation for oral use	428:487	In this study, we report the results of developing a microgranulated binary biomedical preparation for oral use.					
35408695	4	35	theme	sodium	880:885	arg1	diclofenac					887:896	sodium diclofenac	880:896	sodium diclofenac	880:896	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	7	36	theme	@	1291:1291	arg1	pectin					1292:1297	AC-RH@pectin	1286:1297	AC-RH@pectin	1286:1297	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	6	37	theme	@	1109:1109	arg1	pectin					1110:1115	AC-RH@pectin	1104:1115	AC-RH@pectin	1104:1115	Adsorption kinetics of lead and diclofenac adsorption by AC-RH, pectin, and AC-RH@pectin, fitted well a pseudo-second-order model.					
35408695	6	38	theme	Adsorption	1028:1037	arg1	kinetics					1039:1046	Adsorption kinetics	1028:1046	Adsorption kinetics of lead	1028:1054	Adsorption kinetics of lead and diclofenac adsorption by AC-RH, pectin, and AC-RH@pectin, fitted well a pseudo-second-order model.					
35408695	2	39	theme	microgranulated	430:444	arg1	preparation					464:474	a microgranulated binary biomedical preparation	428:474	a microgranulated binary biomedical preparation for oral use	428:487	In this study, we report the results of developing a microgranulated binary biomedical preparation for oral use.					
35408695	1	40	theme	attractive	294:303	arg1	protection					319:328	an attractive complementary protection	291:328	an attractive complementary protection of humans against acute and chronic poisoning	291:374	The use of enterosorbents-materials which can be administered orally and eliminate toxic substances from the gastrointestinal tract (GIT) by sorption-offers an attractive complementary protection of humans against acute and chronic poisoning.					
35408695	0	41	theme	Sodium	115:120	arg1	Xenobiotics					92:102	Xenobiotics Pb(II) and Sodium Diclofenac	92:131	Xenobiotics Pb(II) and Sodium Diclofenac	92:131	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	0	41	theme	Sodium	115:120	arg1	Diclofenac					122:131	Sodium Diclofenac	115:131	Sodium Diclofenac	115:131	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	3	42	theme	core-shell	513:522	arg1	structure					524:532	a core-shell structure	511:532	a core-shell structure based on pectin with low degree of esterification	511:582	It was designed with a core-shell structure based on pectin with low degree of esterification as the core, and nanoporous activated carbon produced from rice husk, AC-RH, as the shell, designated as AC-RH@pectin.					
35408695	0	43	theme	Human	52:56	arg1	Protection					58:67	Human Protection	52:67	Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac	52:131	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	1	44	theme	complementary	305:317	arg1	protection					319:328	an attractive complementary protection	291:328	an attractive complementary protection of humans against acute and chronic poisoning	291:374	The use of enterosorbents-materials which can be administered orally and eliminate toxic substances from the gastrointestinal tract (GIT) by sorption-offers an attractive complementary protection of humans against acute and chronic poisoning.					
35408695	5	45	theme	adsorption	979:988	arg1	capacity					990:997	high adsorption capacity	974:997	high adsorption capacity for both adsorbates studied	974:1025	The composite enterosorbent demonstrated high adsorption capacity for both adsorbates studied.					
35408695	1	46	theme	toxic	217:221	arg1	substances					223:232	toxic substances	217:232	toxic substances	217:232	The use of enterosorbents-materials which can be administered orally and eliminate toxic substances from the gastrointestinal tract (GIT) by sorption-offers an attractive complementary protection of humans against acute and chronic poisoning.					
35408695	4	47	theme	diclofenac	887:896	arg1	properties					718:727	The adsorption properties	703:727	The adsorption properties of the synthesized materials	703:756	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	4	47	theme	diclofenac	887:896	arg1	representative					834:847	a representative	832:847	a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug	832:930	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	7	48	theme	maximum	1248:1254	arg1	qmax					1277:1280	qmax	1277:1280	qmax	1277:1280	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	48	theme	maximum	1248:1254	arg1	capacity					1267:1274	the maximum adsorption capacity	1244:1274	the maximum adsorption capacity	1244:1274	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	48	theme	maximum	1248:1254	arg1	mg/g					1342:1345	130.9 mg/g and 227.8 mg/g	1336:1360	mg/g	1342:1345	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	2	49	theme	oral	480:483	arg1	use					485:487	oral use	480:487	oral use	480:487	In this study, we report the results of developing a microgranulated binary biomedical preparation for oral use.					
35408695	4	50	theme	toxic	852:856	arg1	metals					869:874	toxic polyvalent metals	852:874	toxic polyvalent metals	852:874	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	3	51	theme	rice	643:646	arg1	husk					648:651	rice husk	643:651	rice husk	643:651	It was designed with a core-shell structure based on pectin with low degree of esterification as the core, and nanoporous activated carbon produced from rice husk, AC-RH, as the shell, designated as AC-RH@pectin.					
35408695	3	51	theme	rice	643:646	arg1	AC-RH					654:658	AC-RH	654:658	AC-RH	654:658	It was designed with a core-shell structure based on pectin with low degree of esterification as the core, and nanoporous activated carbon produced from rice husk, AC-RH, as the shell, designated as AC-RH@pectin.					
35408695	9	52	theme	different	1905:1913	arg1	nature					1924:1929	different chemical nature	1905:1929	different chemical nature	1905:1929	Therefore, the composite material AC-RH@pectin demonstrated substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature.					
35408695	1	53	theme	humans	333:338	arg1	protection					319:328	an attractive complementary protection	291:328	an attractive complementary protection of humans against acute and chronic poisoning	291:374	The use of enterosorbents-materials which can be administered orally and eliminate toxic substances from the gastrointestinal tract (GIT) by sorption-offers an attractive complementary protection of humans against acute and chronic poisoning.					
35408695	9	54	theme	chemical	1915:1922	arg1	nature					1924:1929	different chemical nature	1905:1929	different chemical nature	1905:1929	Therefore, the composite material AC-RH@pectin demonstrated substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature.					
35408695	9	55	theme	nature	1924:1929	arg1	substances					1891:1900	substances	1891:1900	substances of different chemical nature	1891:1929	Therefore, the composite material AC-RH@pectin demonstrated substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature.					
35408695	4	56	theme	aqueous	774:780	arg1	solutions					782:790	aqueous solutions	774:790	aqueous solutions for the removal of lead (II) nitrate	774:827	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	4	57	theme	metals	869:874	arg1	properties					718:727	The adsorption properties	703:727	The adsorption properties of the synthesized materials	703:756	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	4	57	theme	metals	869:874	arg1	representative					834:847	a representative	832:847	a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug	832:930	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	7	58	theme	pectin	1292:1297	arg1	qmax					1277:1280	qmax	1277:1280	qmax	1277:1280	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	58	theme	pectin	1292:1297	arg1	capacity					1267:1274	the maximum adsorption capacity	1244:1274	the maximum adsorption capacity	1244:1274	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	58	theme	pectin	1292:1297	arg1	mg/g					1342:1345	130.9 mg/g and 227.8 mg/g	1336:1360	mg/g	1342:1345	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	5	59	theme	composite	937:945	arg1	enterosorbent					947:959	The composite enterosorbent	933:959	The composite enterosorbent	933:959	The composite enterosorbent demonstrated high adsorption capacity for both adsorbates studied.					
35408695	8	60	theme	composite	1586:1594	arg1	pectin					1602:1607	the composite AC-RH@pectin	1582:1607	the composite AC-RH@pectin	1582:1607	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	7	61	theme	fitted	1221:1226	arg1	model					1205:1209	the Langmuir adsorption isotherm model	1172:1209	the Langmuir adsorption isotherm model	1172:1209	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	61	theme	fitted	1221:1226	arg1	model					1237:1241	the best fitted isotherm model	1212:1241	the best fitted isotherm model	1212:1241	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	8	62	theme	adsorption	1499:1508	arg1	capacity					1510:1517	the maximum adsorption capacity	1487:1517	the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g,	1487:1552	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	8	62	theme	adsorption	1499:1508	arg1	lower					1563:1567	lower	1563:1567	lower	1563:1567	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	4	63	theme	polyvalent	858:867	arg1	metals					869:874	toxic polyvalent metals	852:874	toxic polyvalent metals	852:874	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	8	64	theme	AC-RH	1394:1398	arg1	qmax					1386:1389	qmax	1386:1389	qmax of AC-RH for diclofenac	1386:1413	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	8	64	theme	AC-RH	1394:1398	arg1	mg/g					1422:1425	537.6 mg/g	1416:1425	537.6 mg/g	1416:1425	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	8	64	theme	AC-RH	1394:1398	arg1	qmax					1432:1435	qmax	1432:1435	qmax	1432:1435	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	6	65	theme	pseudo-second-order	1132:1150	arg1	model					1152:1156	a pseudo-second-order model	1130:1156	a pseudo-second-order model	1130:1156	Adsorption kinetics of lead and diclofenac adsorption by AC-RH, pectin, and AC-RH@pectin, fitted well a pseudo-second-order model.					
35408695	8	66	theme	AC-RH	1596:1600	arg1	pectin					1602:1607	the composite AC-RH@pectin	1582:1607	the composite AC-RH@pectin	1582:1607	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	7	67	theme	isotherm	1228:1235	arg1	model					1205:1209	the Langmuir adsorption isotherm model	1172:1209	the Langmuir adsorption isotherm model	1172:1209	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	67	theme	isotherm	1228:1235	arg1	model					1237:1241	the best fitted isotherm model	1212:1241	the best fitted isotherm model	1212:1241	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	8	68	theme	AC-RH	1522:1526	arg1	capacity					1510:1517	the maximum adsorption capacity	1487:1517	the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g,	1487:1552	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	8	68	theme	AC-RH	1522:1526	arg1	lower					1563:1567	lower	1563:1567	lower	1563:1567	Although qmax of AC-RH for diclofenac, 537.6 mg/g, and qmax of pectin for lead (II), 245.7 mg/g, were higher, the maximum adsorption capacity of AC-RH for lead (II), 52.7 mg/g, was much lower than that of the composite AC-RH@pectin and the adsorption capacity of pectin for diclofenac was negligible.					
35408695	4	69	theme	drug	927:930	arg1	example					904:910	an example	901:910	an example of a medicinal drug	901:930	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	9	70	theme	material	1703:1710	arg1	pectin					1718:1723	the composite material AC-RH@pectin	1689:1723	the composite material AC-RH@pectin	1689:1723	Therefore, the composite material AC-RH@pectin demonstrated substantial efficiency of removing both species which potentially defines it as a more universal enterosorbent suitable for treating poisoning caused by substances of different chemical nature.					
35408695	7	71	theme	AC-RH	1286:1290	arg1	pectin					1292:1297	AC-RH@pectin	1286:1297	AC-RH@pectin	1286:1297	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	72	theme	adsorption	1256:1265	arg1	qmax					1277:1280	qmax	1277:1280	qmax	1277:1280	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	72	theme	adsorption	1256:1265	arg1	capacity					1267:1274	the maximum adsorption capacity	1244:1274	the maximum adsorption capacity	1244:1274	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	72	theme	adsorption	1256:1265	arg1	mg/g					1342:1345	130.9 mg/g and 227.8 mg/g	1336:1360	mg/g	1342:1345	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	73	theme	isotherm	1196:1203	arg1	model					1205:1209	the Langmuir adsorption isotherm model	1172:1209	the Langmuir adsorption isotherm model	1172:1209	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	73	theme	isotherm	1196:1203	arg1	model					1237:1241	the best fitted isotherm model	1212:1241	the best fitted isotherm model	1212:1241	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	1	74	theme	gastrointestinal	243:258	arg1	GIT					267:269	GIT	267:269	GIT	267:269	The use of enterosorbents-materials which can be administered orally and eliminate toxic substances from the gastrointestinal tract (GIT) by sorption-offers an attractive complementary protection of humans against acute and chronic poisoning.					
35408695	1	74	theme	gastrointestinal	243:258	arg1	tract					260:264	the gastrointestinal tract	239:264	the gastrointestinal tract (GIT)	239:270	The use of enterosorbents-materials which can be administered orally and eliminate toxic substances from the gastrointestinal tract (GIT) by sorption-offers an attractive complementary protection of humans against acute and chronic poisoning.					
35408695	4	75	theme	medicinal	917:925	arg1	drug					927:930	a medicinal drug	915:930	a medicinal drug	915:930	The adsorption properties of the synthesized materials were studied in aqueous solutions for the removal of lead (II) nitrate as a representative of toxic polyvalent metals and sodium diclofenac as an example of a medicinal drug.					
35408695	1	76	theme	acute	348:352	arg1	poisoning					366:374	acute and chronic poisoning	348:374	acute and chronic poisoning	348:374	The use of enterosorbents-materials which can be administered orally and eliminate toxic substances from the gastrointestinal tract (GIT) by sorption-offers an attractive complementary protection of humans against acute and chronic poisoning.					
35408695	0	77	theme	Composite	24:32	arg1	Enterosorbent					34:46	Carbon/Pectin Composite Enterosorbent	10:46	Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac	10:131	Activated Carbon/Pectin Composite Enterosorbent for Human Protection from Intoxication with Xenobiotics Pb(II) and Sodium Diclofenac.					
35408695	7	78	theme	best	1216:1219	arg1	model					1205:1209	the Langmuir adsorption isotherm model	1172:1209	the Langmuir adsorption isotherm model	1172:1209	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
35408695	7	78	theme	best	1216:1219	arg1	model					1237:1241	the best fitted isotherm model	1212:1241	the best fitted isotherm model	1212:1241	According to the Langmuir adsorption isotherm model, the best fitted isotherm model, the maximum adsorption capacity, qmax, of AC-RH@pectin for diclofenac and for lead (II) was 130.9 mg/g and 227.8 mg/g, respectively.					
36183883	0	0	theme	blue	113:116	arg1	adsorption					118:127	methylene blue adsorption	103:127	methylene blue adsorption	103:127	Synthesis of activated carbon-surfactant modified montmorillonite clay-alginate composite membrane for methylene blue adsorption.					
36183883	3	1	theme	membrane	668:675	arg1	efficiency					654:663	the adsorption efficiency	639:663	the adsorption efficiency of membrane	639:675	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	7	2	theme	Thermodynamic	965:977	arg1	study					979:983	Thermodynamic study	965:983	Thermodynamic study	965:983	Thermodynamic study confirmed endothermic and spontaneous MB adsorption on the adsorbent.					
36183883	1	3	theme	benzyl	251:256	arg1	clay					313:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	3	4	theme	adsorption	643:652	arg1	efficiency					654:663	the adsorption efficiency	639:663	the adsorption efficiency of membrane	639:675	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	3	5	theme	temperature	617:627	arg1	effect					629:634	initial solution pH, contact time, membrane dose, MB concentrations and temperature effect	545:634	initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane	545:675	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	1	6	theme	dodycyel	258:265	arg1	clay					313:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	1	7	theme	sulphate	267:274	arg1	clay					313:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	6	8	theme	aqueous	947:953	arg1	solution					955:962	aqueous solution	947:962	aqueous solution	947:962	The adsorption capacity of membrane for MB was 1429 mg/g from aqueous solution.					
36183883	4	9	theme	synthesized	721:731	arg1	membrane					733:740	the synthesized membrane	717:740	the synthesized membrane	717:740	MB dye adsorption on the synthesized membrane was explained well by pseudo second order equation.					
36183883	3	10	theme	Several	514:520	arg1	factors					532:538	Several important factors	514:538	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane	514:675	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	5	11	theme	adsorption	824:833	arg1	data					835:838	MB adsorption data	821:838	MB adsorption data	821:838	Isotherm study showed that MB adsorption data followed Langmuir adsorption isotherm model.					
36183883	9	12	from	media	1244:1248	arg1	adsorption					1220:1229	MB adsorption	1217:1229	MB adsorption from aqueous media	1217:1248	The synthesized membrane proved an efficient adsorbent for MB adsorption from aqueous media.					
36183883	4	13	theme	order	778:782	arg1	equation					784:791	pseudo second order equation	764:791	pseudo second order equation	764:791	MB dye adsorption on the synthesized membrane was explained well by pseudo second order equation.					
36183883	4	14	theme	second	771:776	arg1	equation					784:791	pseudo second order equation	764:791	pseudo second order equation	764:791	MB dye adsorption on the synthesized membrane was explained well by pseudo second order equation.					
36183883	9	15	theme	MB	1217:1218	arg1	adsorption					1220:1229	MB adsorption	1217:1229	MB adsorption from aqueous media	1217:1248	The synthesized membrane proved an efficient adsorbent for MB adsorption from aqueous media.					
36183883	8	16	theme	path	1071:1074	arg1	way					1076:1078	The mechanistic path way	1055:1078	The mechanistic path way	1055:1078	The mechanistic path way indicated that electrostatic forces were involved in this adsorption process.					
36183883	1	17	theme	activated	201:209	arg1	AC					219:220	AC	219:220	AC	219:220	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	1	17	theme	activated	201:209	arg1	carbon					211:216	activated carbon	201:216	activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye	201:381	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	6	18	theme	adsorption	889:898	arg1	capacity					900:907	The adsorption capacity	885:907	The adsorption capacity of membrane for MB	885:926	The adsorption capacity of membrane for MB was 1429 mg/g from aqueous solution.					
36183883	1	19	theme	SBDS	277:280	arg1	clay					313:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	6	20	theme	membrane	912:919	arg1	capacity					900:907	The adsorption capacity	885:907	The adsorption capacity of membrane for MB	885:926	The adsorption capacity of membrane for MB was 1429 mg/g from aqueous solution.					
36183883	4	21	from	adsorption	703:712	arg1	membrane					733:740	the synthesized membrane	717:740	the synthesized membrane	717:740	MB dye adsorption on the synthesized membrane was explained well by pseudo second order equation.					
36183883	3	22	theme	initial	545:551	arg1	effect					629:634	initial solution pH, contact time, membrane dose, MB concentrations and temperature effect	545:634	initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane	545:675	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	5	23	theme	Langmuir	849:856	arg1	adsorption					858:867	Langmuir adsorption	849:867	Langmuir adsorption isotherm model	849:882	Isotherm study showed that MB adsorption data followed Langmuir adsorption isotherm model.					
36183883	0	24	theme	carbon-surfactant	23:39	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of activated carbon-surfactant	0:39	Synthesis of activated carbon-surfactant modified montmorillonite clay-alginate composite membrane for methylene blue adsorption.					
36183883	4	25	theme	dye	699:701	arg1	adsorption					703:712	MB dye adsorption	696:712	MB dye adsorption on the synthesized membrane	696:740	MB dye adsorption on the synthesized membrane was explained well by pseudo second order equation.					
36183883	9	26	theme	aqueous	1236:1242	arg1	media					1244:1248	aqueous media	1236:1248	aqueous media	1236:1248	The synthesized membrane proved an efficient adsorbent for MB adsorption from aqueous media.					
36183883	3	27	theme	important	522:530	arg1	factors					532:538	Several important factors	514:538	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane	514:675	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	0	28	theme	activated	13:21	arg1	carbon-surfactant					23:39	activated carbon-surfactant	13:39	activated carbon-surfactant	13:39	Synthesis of activated carbon-surfactant modified montmorillonite clay-alginate composite membrane for methylene blue adsorption.					
36183883	1	29	theme	treated	283:289	arg1	clay					313:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	3	30	theme	concentrations	598:611	arg1	effect					629:634	initial solution pH, contact time, membrane dose, MB concentrations and temperature effect	545:634	initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane	545:675	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	5	31	theme	adsorption	858:867	arg1	model					878:882	Langmuir adsorption isotherm model	849:882	Langmuir adsorption isotherm model	849:882	Isotherm study showed that MB adsorption data followed Langmuir adsorption isotherm model.					
36183883	0	32	theme	montmorillonite	50:64	arg1	membrane					90:97	montmorillonite clay-alginate composite membrane	50:97	montmorillonite clay-alginate composite membrane for methylene blue adsorption	50:127	Synthesis of activated carbon-surfactant modified montmorillonite clay-alginate composite membrane for methylene blue adsorption.					
36183883	1	33	theme	montmorillonite	291:305	arg1	clay					313:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	8	34	theme	electrostatic	1095:1107	arg1	forces					1109:1114	electrostatic forces	1095:1114	electrostatic forces	1095:1114	The mechanistic path way indicated that electrostatic forces were involved in this adsorption process.					
36183883	1	35	theme	methylene-blue	359:372	arg1	dye					379:381	methylene-blue (MB) dye	359:381	methylene-blue (MB) dye	359:381	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	1	35	theme	methylene-blue	359:372	arg1	MB					375:376	MB	375:376	MB	375:376	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	2	36	theme	analytical	444:453	arg1	FTIR					481:484	FTIR	481:484	FTIR	481:484	The AC-MMT-alg composite membranes were characterized using analytical characterizations such as FTIR, SEM, EDX and TGA analysis.					
36183883	2	36	theme	analytical	444:453	arg1	EDX					492:494	EDX	492:494	EDX	492:494	The AC-MMT-alg composite membranes were characterized using analytical characterizations such as FTIR, SEM, EDX and TGA analysis.					
36183883	2	36	theme	analytical	444:453	arg1	analysis					504:511	TGA analysis	500:511	TGA analysis	500:511	The AC-MMT-alg composite membranes were characterized using analytical characterizations such as FTIR, SEM, EDX and TGA analysis.					
36183883	2	36	theme	analytical	444:453	arg1	SEM					487:489	SEM	487:489	SEM	487:489	The AC-MMT-alg composite membranes were characterized using analytical characterizations such as FTIR, SEM, EDX and TGA analysis.					
36183883	2	36	theme	analytical	444:453	arg1	characterizations					455:471	analytical characterizations	444:471	analytical characterizations such as FTIR, SEM, EDX and TGA analysis	444:511	The AC-MMT-alg composite membranes were characterized using analytical characterizations such as FTIR, SEM, EDX and TGA analysis.					
36183883	2	37	theme	TGA	500:502	arg1	analysis					504:511	TGA analysis	500:511	TGA analysis	500:511	The AC-MMT-alg composite membranes were characterized using analytical characterizations such as FTIR, SEM, EDX and TGA analysis.					
36183883	2	38	theme	composite	399:407	arg1	membranes					409:417	The AC-MMT-alg composite membranes	384:417	The AC-MMT-alg composite membranes	384:417	The AC-MMT-alg composite membranes were characterized using analytical characterizations such as FTIR, SEM, EDX and TGA analysis.					
36183883	7	39	theme	spontaneous	1011:1021	arg1	adsorption					1026:1035	endothermic and spontaneous MB adsorption	995:1035	endothermic and spontaneous MB adsorption	995:1035	Thermodynamic study confirmed endothermic and spontaneous MB adsorption on the adsorbent.					
36183883	5	40	theme	MB	821:822	arg1	data					835:838	MB adsorption data	821:838	MB adsorption data	821:838	Isotherm study showed that MB adsorption data followed Langmuir adsorption isotherm model.					
36183883	0	41	theme	composite	80:88	arg1	membrane					90:97	montmorillonite clay-alginate composite membrane	50:97	montmorillonite clay-alginate composite membrane for methylene blue adsorption	50:127	Synthesis of activated carbon-surfactant modified montmorillonite clay-alginate composite membrane for methylene blue adsorption.					
36183883	1	42	theme	MMT	308:310	arg1	clay					313:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	5	43	theme	Isotherm	794:801	arg1	study					803:807	Isotherm study	794:807	Isotherm study	794:807	Isotherm study showed that MB adsorption data followed Langmuir adsorption isotherm model.					
36183883	3	44	theme	MB	595:596	arg1	concentrations					598:611	MB concentrations	595:611	MB concentrations	595:611	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	3	45	theme	solution	553:560	arg1	pH					562:563	solution pH	553:563	solution pH	553:563	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	7	46	theme	MB	1023:1024	arg1	adsorption					1026:1035	endothermic and spontaneous MB adsorption	995:1035	endothermic and spontaneous MB adsorption	995:1035	Thermodynamic study confirmed endothermic and spontaneous MB adsorption on the adsorbent.					
36183883	1	47	theme	research	138:145	arg1	work					147:150	this research work	133:150	this research work	133:150	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	0	48	theme	clay-alginate	66:78	arg1	membrane					90:97	montmorillonite clay-alginate composite membrane	50:97	montmorillonite clay-alginate composite membrane for methylene blue adsorption	50:127	Synthesis of activated carbon-surfactant modified montmorillonite clay-alginate composite membrane for methylene blue adsorption.					
36183883	3	49	theme	pH	562:563	arg1	effect					629:634	initial solution pH, contact time, membrane dose, MB concentrations and temperature effect	545:634	initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane	545:675	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	7	50	theme	endothermic	995:1005	arg1	adsorption					1026:1035	endothermic and spontaneous MB adsorption	995:1035	endothermic and spontaneous MB adsorption	995:1035	Thermodynamic study confirmed endothermic and spontaneous MB adsorption on the adsorbent.					
36183883	4	51	theme	pseudo	764:769	arg1	equation					784:791	pseudo second order equation	764:791	pseudo second order equation	764:791	MB dye adsorption on the synthesized membrane was explained well by pseudo second order equation.					
36183883	1	52	theme	dye	379:381	arg1	adsorption					345:354	the adsorption	341:354	the adsorption of methylene-blue (MB) dye	341:381	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	3	53	theme	membrane	580:587	arg1	dose					589:592	membrane dose	580:592	membrane dose	580:592	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	3	54	theme	contact	566:572	arg1	time					574:577	contact time	566:577	contact time	566:577	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	4	55	theme	MB	696:697	arg1	adsorption					703:712	MB dye adsorption	696:712	MB dye adsorption on the synthesized membrane	696:740	MB dye adsorption on the synthesized membrane was explained well by pseudo second order equation.					
36183883	1	56	attach	derived	223:229	arg1	sesban					236:241	sesban	236:241	sesban	236:241	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	1	56	attach	derived	223:229	arg1	clay					313:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	1	56	attach	derived	223:229	arg2	carbon					211:216	activated carbon	201:216	activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye	201:381	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	1	56	attach	derived	223:229	arg1	alginate					322:329	alginate	322:329	alginate (alg)	322:335	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	1	56	attach	derived	223:229	arg2	AC					219:220	AC	219:220	AC	219:220	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	1	56	attach	derived	223:229	arg1	alg					332:334	alg	332:334	alg	332:334	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	3	57	theme	dose	589:592	arg1	effect					629:634	initial solution pH, contact time, membrane dose, MB concentrations and temperature effect	545:634	initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane	545:675	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	5	58	theme	isotherm	869:876	arg1	model					878:882	Langmuir adsorption isotherm model	849:882	Langmuir adsorption isotherm model	849:882	Isotherm study showed that MB adsorption data followed Langmuir adsorption isotherm model.					
36183883	8	59	theme	adsorption	1138:1147	arg1	process					1149:1155	this adsorption process	1133:1155	this adsorption process	1133:1155	The mechanistic path way indicated that electrostatic forces were involved in this adsorption process.					
36183883	8	60	theme	mechanistic	1059:1069	arg1	way					1076:1078	The mechanistic path way	1055:1078	The mechanistic path way	1055:1078	The mechanistic path way indicated that electrostatic forces were involved in this adsorption process.					
36183883	3	61	theme	time	574:577	arg1	effect					629:634	initial solution pH, contact time, membrane dose, MB concentrations and temperature effect	545:634	initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane	545:675	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	9	62	theme	synthesized	1162:1172	arg1	membrane					1174:1181	The synthesized membrane	1158:1181	The synthesized membrane	1158:1181	The synthesized membrane proved an efficient adsorbent for MB adsorption from aqueous media.					
36183883	1	63	theme	novel	155:159	arg1	membrane					171:178	a novel composite membrane	153:178	a novel composite membrane	153:178	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	3	64	from	effect	629:634	arg1	efficiency					654:663	the adsorption efficiency	639:663	the adsorption efficiency of membrane	639:675	Several important factors like initial solution pH, contact time, membrane dose, MB concentrations and temperature effect on the adsorption efficiency of membrane were investigated.					
36183883	2	65	theme	AC-MMT-alg	388:397	arg1	membranes					409:417	The AC-MMT-alg composite membranes	384:417	The AC-MMT-alg composite membranes	384:417	The AC-MMT-alg composite membranes were characterized using analytical characterizations such as FTIR, SEM, EDX and TGA analysis.					
36183883	1	66	theme	composite	161:169	arg1	membrane					171:178	a novel composite membrane	153:178	a novel composite membrane	153:178	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36183883	1	67	theme	sodium	244:249	arg1	clay					313:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay	244:316	In this research work, a novel composite membrane was synthesized from activated carbon (AC) derived from sesban, sodium benzyl dodycyel sulphate (SBDS) treated montmorillonite (MMT) clay and alginate (alg) for the adsorption of methylene-blue (MB) dye.					
36455635	5	0	used	used	590:593	arg2	research					581:588	This research	576:588	This research	576:588	This research used a previously reported method to rapidly synthesize chitin magnetite nanocomposites (ChM) by co-precipitation while irradiating with ultrasound (US).					
36455635	5	1	theme	reported	608:615	arg1	method					617:622	a previously reported method	595:622	a previously reported method	595:622	This research used a previously reported method to rapidly synthesize chitin magnetite nanocomposites (ChM) by co-precipitation while irradiating with ultrasound (US).					
36455635	9	2	theme	Anionic	1300:1306	arg1	dyes					1312:1315	Anionic azo dyes	1300:1315	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites	1300:1407	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	4	3	theme	Azo	546:548	arg1	dyes					550:553	Azo dyes	546:553	Azo dyes	546:553	This research aims to synthesize composite Nanorods and Nanospheres and characterize and test to remove Azo dyes from the wastewater.					
36455635	8	4	mod	modification	1220:1231	arg3	surface					1212:1218	the surface modification	1208:1231	the surface modification of ChMs	1208:1239	The nitrogen adsorption-desorption isotherms showed that the surface modification of ChMs resulted in a rise of specific surface area and pore size.					
36455635	8	4	mod	modification	1220:1231	arg1	ChMs					1236:1239	ChMs	1236:1239	ChMs	1236:1239	The nitrogen adsorption-desorption isotherms showed that the surface modification of ChMs resulted in a rise of specific surface area and pore size.					
36455635	4	5	theme	composite	475:483	arg1	Nanorods					485:492	composite Nanorods	475:492	composite Nanorods	475:492	This research aims to synthesize composite Nanorods and Nanospheres and characterize and test to remove Azo dyes from the wastewater.					
36455635	9	6	theme	surface-modified	1482:1497	arg1	samples					1499:1505	surface-modified samples	1482:1505	surface-modified samples at pH 4	1482:1513	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	7	7	theme	magnetization	1105:1117	arg1	values					1119:1124	the superparamagnetic performance and saturation magnetization values	1056:1124	the superparamagnetic performance and saturation magnetization values between 45 and 58 emu/g	1056:1148	Both the morphologies displayed a nanoscale limit with particles averaging between 5 and 30 nm in size, resulting the superparamagnetic performance and saturation magnetization values between 45 and 58 emu/g.					
36455635	6	8	theme	reaction	882:889	arg1	time					891:894	the reaction time	878:894	the reaction time to 8 min	878:903	Detailed structural characterization of ChM revealed a crystalline phase analogous to magnetite and spherical morphologies; extending the reaction time to 8 min yielded a "nanorod" type morphology.					
36455635	7	9	theme	saturation	1094:1103	arg1	magnetization					1105:1117	saturation magnetization	1094:1117	saturation magnetization	1094:1117	Both the morphologies displayed a nanoscale limit with particles averaging between 5 and 30 nm in size, resulting the superparamagnetic performance and saturation magnetization values between 45 and 58 emu/g.					
36455635	6	10	theme	ChM	784:786	arg1	characterization					764:779	Detailed structural characterization	744:779	Detailed structural characterization of ChM	744:786	Detailed structural characterization of ChM revealed a crystalline phase analogous to magnetite and spherical morphologies; extending the reaction time to 8 min yielded a "nanorod" type morphology.					
36455635	10	11	theme	distinct	1646:1653	arg1	processes					1666:1674	two distinct adsorption processes	1642:1674	two distinct adsorption processes	1642:1674	Adsorption capacity studies showed that molecule size effect and electrostatic attraction were two distinct adsorption processes for unmodified and modified ChMs.					
36455635	8	12	theme	adsorption-desorption	1164:1184	arg1	isotherms					1186:1194	The nitrogen adsorption-desorption isotherms	1151:1194	The nitrogen adsorption-desorption isotherms	1151:1194	The nitrogen adsorption-desorption isotherms showed that the surface modification of ChMs resulted in a rise of specific surface area and pore size.					
36455635	9	13	theme	azo	1308:1310	arg1	dyes					1312:1315	Anionic azo dyes	1300:1315	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites	1300:1407	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	7	14	theme	superparamagnetic	1060:1076	arg1	performance					1078:1088	superparamagnetic performance	1060:1088	superparamagnetic performance	1060:1088	Both the morphologies displayed a nanoscale limit with particles averaging between 5 and 30 nm in size, resulting the superparamagnetic performance and saturation magnetization values between 45 and 58 emu/g.					
36455635	1	15	theme	development	184:194	arg1	goals					196:200	11 worldwide sustainable development goals	159:200	11 worldwide sustainable development goals	159:200	Water treatment is as much important as it is to satisfying 11 worldwide sustainable development goals out of 17.					
36455635	1	16	theme	worldwide	162:170	arg1	goals					196:200	11 worldwide sustainable development goals	159:200	11 worldwide sustainable development goals	159:200	Water treatment is as much important as it is to satisfying 11 worldwide sustainable development goals out of 17.					
36455635	8	17	theme	nitrogen	1155:1162	arg1	isotherms					1186:1194	The nitrogen adsorption-desorption isotherms	1151:1194	The nitrogen adsorption-desorption isotherms	1151:1194	The nitrogen adsorption-desorption isotherms showed that the surface modification of ChMs resulted in a rise of specific surface area and pore size.					
36455635	7	18	theme	performance	1078:1088	arg1	values					1119:1124	the superparamagnetic performance and saturation magnetization values	1056:1124	the superparamagnetic performance and saturation magnetization values between 45 and 58 emu/g	1056:1148	Both the morphologies displayed a nanoscale limit with particles averaging between 5 and 30 nm in size, resulting the superparamagnetic performance and saturation magnetization values between 45 and 58 emu/g.					
36455635	1	19	theme	sustainable	172:182	arg1	goals					196:200	11 worldwide sustainable development goals	159:200	11 worldwide sustainable development goals	159:200	Water treatment is as much important as it is to satisfying 11 worldwide sustainable development goals out of 17.					
36455635	0	20	theme	porous	9:14	arg1	nanostructure					28:40	Magnetic porous Ag2O/Chitin nanostructure	0:40	Magnetic porous Ag2O/Chitin nanostructure	0:40	Magnetic porous Ag2O/Chitin nanostructure adsorbent for eco-friendly effective disposing azo dyes.					
36455635	9	21	with	pH-dependent	1437:1448	arg1	reaction					1460:1467	the reaction	1456:1467	the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8	1456:1544	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	6	22	theme	structural	753:762	arg1	characterization					764:779	Detailed structural characterization	744:779	Detailed structural characterization of ChM	744:786	Detailed structural characterization of ChM revealed a crystalline phase analogous to magnetite and spherical morphologies; extending the reaction time to 8 min yielded a "nanorod" type morphology.					
36455635	1	23	theme	Water	99:103	arg1	treatment					105:113	Water treatment	99:113	Water treatment	99:113	Water treatment is as much important as it is to satisfying 11 worldwide sustainable development goals out of 17.					
36455635	3	24	theme	Azo	382:384	arg1	Production					401:410	Azo contained Dyes Production	382:410	Azo contained Dyes Production	382:410	Azo contained Dyes Production was banned in many countries.					
36455635	0	25	theme	Magnetic	0:7	arg1	nanostructure					28:40	Magnetic porous Ag2O/Chitin nanostructure	0:40	Magnetic porous Ag2O/Chitin nanostructure	0:40	Magnetic porous Ag2O/Chitin nanostructure adsorbent for eco-friendly effective disposing azo dyes.					
36455635	9	26	theme	methyl	1318:1323	arg1	RB5					1359:1361	RB5	1359:1361	RB5	1359:1361	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	9	26	theme	methyl	1318:1323	arg1	MO					1333:1334	MO	1333:1334	MO	1333:1334	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	9	26	theme	methyl	1318:1323	arg1	orange					1325:1330	methyl orange	1318:1330	methyl orange (MO)	1318:1335	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	11	27	dep	a	1754:1754	arg1	substitute					1756:1765	substitute	1756:1765	substitute	1756:1765	Chitin Magnetite nanoparticles appear to be a substitute for traditional anionic dye adsorbents.					
36455635	6	28	theme	Detailed	744:751	arg1	characterization					764:779	Detailed structural characterization	744:779	Detailed structural characterization of ChM	744:786	Detailed structural characterization of ChM revealed a crystalline phase analogous to magnetite and spherical morphologies; extending the reaction time to 8 min yielded a "nanorod" type morphology.					
36455635	9	29	theme	unmodified	1519:1528	arg1	samples					1530:1536	unmodified samples	1519:1536	unmodified samples at pH 8	1519:1544	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	3	30	theme	contained	386:394	arg1	Production					401:410	Azo contained Dyes Production	382:410	Azo contained Dyes Production	382:410	Azo contained Dyes Production was banned in many countries.					
36455635	11	31	theme	anionic	1783:1789	arg1	adsorbents					1795:1804	traditional anionic dye adsorbents	1771:1804	traditional anionic dye adsorbents	1771:1804	Chitin Magnetite nanoparticles appear to be a substitute for traditional anionic dye adsorbents.					
36455635	10	32	theme	modified	1695:1702	arg1	ChMs					1704:1707	unmodified and modified ChMs	1680:1707	unmodified and modified ChMs	1680:1707	Adsorption capacity studies showed that molecule size effect and electrostatic attraction were two distinct adsorption processes for unmodified and modified ChMs.					
36455635	10	33	theme	size	1596:1599	arg1	effect					1601:1606	molecule size effect	1587:1606	molecule size effect	1587:1606	Adsorption capacity studies showed that molecule size effect and electrostatic attraction were two distinct adsorption processes for unmodified and modified ChMs.					
36455635	2	34	theme	Azo	228:230	arg1	important					240:248	important	240:248	important	240:248	The removal of Azo is much important as they are toxic and their existence in water, air and food can easily affect humans by triggering allergies, forming tumours etc.					
36455635	2	34	theme	Azo	228:230	arg1	removal					217:223	The removal	213:223	The removal of Azo	213:230	The removal of Azo is much important as they are toxic and their existence in water, air and food can easily affect humans by triggering allergies, forming tumours etc.					
36455635	8	35	theme	specific	1263:1270	arg1	area					1280:1283	specific surface area	1263:1283	specific surface area	1263:1283	The nitrogen adsorption-desorption isotherms showed that the surface modification of ChMs resulted in a rise of specific surface area and pore size.					
36455635	0	36	theme	Ag2O/Chitin	16:26	arg1	nanostructure					28:40	Magnetic porous Ag2O/Chitin nanostructure	0:40	Magnetic porous Ag2O/Chitin nanostructure	0:40	Magnetic porous Ag2O/Chitin nanostructure adsorbent for eco-friendly effective disposing azo dyes.					
36455635	12	37	theme	materials	1833:1841	arg1	sources					1843:1849	the two key materials sources	1821:1849	the two key materials sources	1821:1849	Additionally, the two key materials sources, chitin, and magnetite are inexpensive and easily accessible.					
36455635	12	37	theme	materials	1833:1841	arg1	inexpensive					1878:1888	inexpensive	1878:1888	inexpensive	1878:1888	Additionally, the two key materials sources, chitin, and magnetite are inexpensive and easily accessible.					
36455635	10	38	theme	unmodified	1680:1689	arg1	ChMs					1704:1707	unmodified and modified ChMs	1680:1707	unmodified and modified ChMs	1680:1707	Adsorption capacity studies showed that molecule size effect and electrostatic attraction were two distinct adsorption processes for unmodified and modified ChMs.					
36455635	9	39	from	pH	1541:1542	arg1	samples					1499:1505	surface-modified samples	1482:1505	surface-modified samples at pH 4	1482:1513	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	9	39	from	pH	1541:1542	arg1	samples					1530:1536	unmodified samples	1519:1536	unmodified samples at pH 8	1519:1544	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	3	40	theme	many	426:429	arg1	countries					431:439	many countries	426:439	many countries	426:439	Azo contained Dyes Production was banned in many countries.					
36455635	6	41	theme	type	925:928	arg1	morphology					930:939	a "nanorod" type morphology	913:939	a "nanorod" type morphology	913:939	Detailed structural characterization of ChM revealed a crystalline phase analogous to magnetite and spherical morphologies; extending the reaction time to 8 min yielded a "nanorod" type morphology.					
36455635	5	42	dep	ultrasound	727:736	arg1	US					739:740	US	739:740	US	739:740	This research used a previously reported method to rapidly synthesize chitin magnetite nanocomposites (ChM) by co-precipitation while irradiating with ultrasound (US).					
36455635	6	43	theme	spherical	844:852	arg1	morphologies					854:865	magnetite and spherical morphologies	830:865	magnetite and spherical morphologies	830:865	Detailed structural characterization of ChM revealed a crystalline phase analogous to magnetite and spherical morphologies; extending the reaction time to 8 min yielded a "nanorod" type morphology.					
36455635	2	44	dep	tumours	369:375	arg1	etc					377:379	etc	377:379	tumours etc	369:379	The removal of Azo is much important as they are toxic and their existence in water, air and food can easily affect humans by triggering allergies, forming tumours etc.					
36455635	10	45	theme	molecule	1587:1594	arg1	effect					1601:1606	molecule size effect	1587:1606	molecule size effect	1587:1606	Adsorption capacity studies showed that molecule size effect and electrostatic attraction were two distinct adsorption processes for unmodified and modified ChMs.					
36455635	8	46	theme	area	1280:1283	arg1	rise					1255:1258	a rise	1253:1258	a rise of specific surface area and pore size	1253:1297	The nitrogen adsorption-desorption isotherms showed that the surface modification of ChMs resulted in a rise of specific surface area and pore size.					
36455635	3	47	theme	Dyes	396:399	arg1	Production					401:410	Azo contained Dyes Production	382:410	Azo contained Dyes Production	382:410	Azo contained Dyes Production was banned in many countries.					
36455635	6	48	theme	"	923:923	arg1	morphology					930:939	a "nanorod" type morphology	913:939	a "nanorod" type morphology	913:939	Detailed structural characterization of ChM revealed a crystalline phase analogous to magnetite and spherical morphologies; extending the reaction time to 8 min yielded a "nanorod" type morphology.					
36455635	7	49	with	limit	986:990	arg1	particles					997:1005	particles	997:1005	particles averaging between 5 and 30 nm	997:1035	Both the morphologies displayed a nanoscale limit with particles averaging between 5 and 30 nm in size, resulting the superparamagnetic performance and saturation magnetization values between 45 and 58 emu/g.					
36455635	8	50	theme	surface	1272:1278	arg1	area					1280:1283	specific surface area	1263:1283	specific surface area	1263:1283	The nitrogen adsorption-desorption isotherms showed that the surface modification of ChMs resulted in a rise of specific surface area and pore size.					
36455635	0	51	theme	effective	69:77	arg1	dyes					93:96	eco-friendly effective disposing azo dyes	56:96	eco-friendly effective disposing azo dyes	56:96	Magnetic porous Ag2O/Chitin nanostructure adsorbent for eco-friendly effective disposing azo dyes.					
36455635	6	52	theme	nanorod	916:922	arg1	morphology					930:939	a "nanorod" type morphology	913:939	a "nanorod" type morphology	913:939	Detailed structural characterization of ChM revealed a crystalline phase analogous to magnetite and spherical morphologies; extending the reaction time to 8 min yielded a "nanorod" type morphology.					
36455635	8	53	theme	surface	1212:1218	arg1	modification					1220:1231	the surface modification	1208:1231	the surface modification of ChMs	1208:1239	The nitrogen adsorption-desorption isotherms showed that the surface modification of ChMs resulted in a rise of specific surface area and pore size.					
36455635	6	54	theme	magnetite	830:838	arg1	morphologies					854:865	magnetite and spherical morphologies	830:865	magnetite and spherical morphologies	830:865	Detailed structural characterization of ChM revealed a crystalline phase analogous to magnetite and spherical morphologies; extending the reaction time to 8 min yielded a "nanorod" type morphology.					
36455635	12	55	theme	key	1829:1831	arg1	sources					1843:1849	the two key materials sources	1821:1849	the two key materials sources	1821:1849	Additionally, the two key materials sources, chitin, and magnetite are inexpensive and easily accessible.					
36455635	12	55	theme	key	1829:1831	arg1	inexpensive					1878:1888	inexpensive	1878:1888	inexpensive	1878:1888	Additionally, the two key materials sources, chitin, and magnetite are inexpensive and easily accessible.					
36455635	8	56	theme	pore	1289:1292	arg1	size					1294:1297	pore size	1289:1297	pore size	1289:1297	The nitrogen adsorption-desorption isotherms showed that the surface modification of ChMs resulted in a rise of specific surface area and pore size.					
36455635	10	57	theme	capacity	1558:1565	arg1	studies					1567:1573	Adsorption capacity studies	1547:1573	Adsorption capacity studies	1547:1573	Adsorption capacity studies showed that molecule size effect and electrostatic attraction were two distinct adsorption processes for unmodified and modified ChMs.					
36455635	11	58	theme	dye	1791:1793	arg1	adsorbents					1795:1804	traditional anionic dye adsorbents	1771:1804	traditional anionic dye adsorbents	1771:1804	Chitin Magnetite nanoparticles appear to be a substitute for traditional anionic dye adsorbents.					
36455635	5	59	theme	chitin	646:651	arg1	ChM					679:681	ChM	679:681	ChM	679:681	This research used a previously reported method to rapidly synthesize chitin magnetite nanocomposites (ChM) by co-precipitation while irradiating with ultrasound (US).					
36455635	5	59	theme	chitin	646:651	arg1	nanocomposites					663:676	chitin magnetite nanocomposites	646:676	chitin magnetite nanocomposites (ChM)	646:682	This research used a previously reported method to rapidly synthesize chitin magnetite nanocomposites (ChM) by co-precipitation while irradiating with ultrasound (US).					
36455635	7	60	theme	nanoscale	976:984	arg1	limit					986:990	a nanoscale limit	974:990	a nanoscale limit with particles averaging between 5 and 30 nm	974:1035	Both the morphologies displayed a nanoscale limit with particles averaging between 5 and 30 nm in size, resulting the superparamagnetic performance and saturation magnetization values between 45 and 58 emu/g.					
36455635	5	61	theme	magnetite	653:661	arg1	ChM					679:681	ChM	679:681	ChM	679:681	This research used a previously reported method to rapidly synthesize chitin magnetite nanocomposites (ChM) by co-precipitation while irradiating with ultrasound (US).					
36455635	5	61	theme	magnetite	653:661	arg1	nanocomposites					663:676	chitin magnetite nanocomposites	646:676	chitin magnetite nanocomposites (ChM)	646:682	This research used a previously reported method to rapidly synthesize chitin magnetite nanocomposites (ChM) by co-precipitation while irradiating with ultrasound (US).					
36455635	6	62	theme	analogous	817:825	arg1	phase					811:815	a crystalline phase	797:815	a crystalline phase analogous to magnetite and spherical morphologies	797:865	Detailed structural characterization of ChM revealed a crystalline phase analogous to magnetite and spherical morphologies; extending the reaction time to 8 min yielded a "nanorod" type morphology.					
36455635	1	63	dep	17	209:210	arg1	out					202:204	out	202:204	out	202:204	Water treatment is as much important as it is to satisfying 11 worldwide sustainable development goals out of 17.					
36455635	11	64	theme	traditional	1771:1781	arg1	adsorbents					1795:1804	traditional anionic dye adsorbents	1771:1804	traditional anionic dye adsorbents	1771:1804	Chitin Magnetite nanoparticles appear to be a substitute for traditional anionic dye adsorbents.					
36455635	9	65	dep	dyes	1312:1315	arg1	RB5					1359:1361	RB5	1359:1361	RB5	1359:1361	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	9	65	dep	dyes	1312:1315	arg1	adsorption					1365:1374	adsorption	1365:1374	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites	1300:1407	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	9	65	dep	dyes	1312:1315	arg1	5					1356:1356	5	1356:1356	5	1356:1356	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	9	65	dep	dyes	1312:1315	arg1	MO					1333:1334	MO	1333:1334	MO	1333:1334	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	9	65	dep	dyes	1312:1315	arg1	orange					1325:1330	methyl orange	1318:1330	methyl orange (MO)	1318:1335	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	11	66	theme	Magnetite	1717:1725	arg1	nanoparticles					1727:1739	Chitin Magnetite nanoparticles	1710:1739	Chitin Magnetite nanoparticles	1710:1739	Chitin Magnetite nanoparticles appear to be a substitute for traditional anionic dye adsorbents.					
36455635	10	67	theme	electrostatic	1612:1624	arg1	attraction					1626:1635	electrostatic attraction	1612:1635	electrostatic attraction	1612:1635	Adsorption capacity studies showed that molecule size effect and electrostatic attraction were two distinct adsorption processes for unmodified and modified ChMs.					
36455635	8	68	theme	size	1294:1297	arg1	rise					1255:1258	a rise	1253:1258	a rise of specific surface area and pore size	1253:1297	The nitrogen adsorption-desorption isotherms showed that the surface modification of ChMs resulted in a rise of specific surface area and pore size.					
36455635	8	69	theme	ChMs	1236:1239	arg1	modification					1220:1231	the surface modification	1208:1231	the surface modification of ChMs	1208:1239	The nitrogen adsorption-desorption isotherms showed that the surface modification of ChMs resulted in a rise of specific surface area and pore size.					
36455635	10	70	theme	adsorption	1655:1664	arg1	processes					1666:1674	two distinct adsorption processes	1642:1674	two distinct adsorption processes	1642:1674	Adsorption capacity studies showed that molecule size effect and electrostatic attraction were two distinct adsorption processes for unmodified and modified ChMs.					
36455635	0	71	theme	azo	89:91	arg1	dyes					93:96	eco-friendly effective disposing azo dyes	56:96	eco-friendly effective disposing azo dyes	56:96	Magnetic porous Ag2O/Chitin nanostructure adsorbent for eco-friendly effective disposing azo dyes.					
36455635	6	72	theme	crystalline	799:809	arg1	phase					811:815	a crystalline phase	797:815	a crystalline phase analogous to magnetite and spherical morphologies	797:865	Detailed structural characterization of ChM revealed a crystalline phase analogous to magnetite and spherical morphologies; extending the reaction time to 8 min yielded a "nanorod" type morphology.					
36455635	9	73	from	pH	1510:1511	arg1	samples					1499:1505	surface-modified samples	1482:1505	surface-modified samples at pH 4	1482:1513	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	9	73	from	pH	1510:1511	arg1	samples					1530:1536	unmodified samples	1519:1536	unmodified samples at pH 8	1519:1544	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	0	74	theme	disposing	79:87	arg1	dyes					93:96	eco-friendly effective disposing azo dyes	56:96	eco-friendly effective disposing azo dyes	56:96	Magnetic porous Ag2O/Chitin nanostructure adsorbent for eco-friendly effective disposing azo dyes.					
36455635	9	75	theme	nanocomposites	1394:1407	arg1	surface					1383:1389	the surface	1379:1389	the surface of nanocomposites	1379:1407	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	10	76	theme	Adsorption	1547:1556	arg1	studies					1567:1573	Adsorption capacity studies	1547:1573	Adsorption capacity studies	1547:1573	Adsorption capacity studies showed that molecule size effect and electrostatic attraction were two distinct adsorption processes for unmodified and modified ChMs.					
36455635	9	77	from	dyes	1312:1315	arg1	surface					1383:1389	the surface	1379:1389	the surface of nanocomposites	1379:1407	Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8.					
36455635	2	78	from	existence	278:286	arg1	food					306:309	food	306:309	food	306:309	The removal of Azo is much important as they are toxic and their existence in water, air and food can easily affect humans by triggering allergies, forming tumours etc.					
36455635	2	78	from	existence	278:286	arg1	air					298:300	air	298:300	air	298:300	The removal of Azo is much important as they are toxic and their existence in water, air and food can easily affect humans by triggering allergies, forming tumours etc.					
36455635	2	78	from	existence	278:286	arg1	water					291:295	water	291:295	water	291:295	The removal of Azo is much important as they are toxic and their existence in water, air and food can easily affect humans by triggering allergies, forming tumours etc.					
36455635	11	79	theme	Chitin	1710:1715	arg1	nanoparticles					1727:1739	Chitin Magnetite nanoparticles	1710:1739	Chitin Magnetite nanoparticles	1710:1739	Chitin Magnetite nanoparticles appear to be a substitute for traditional anionic dye adsorbents.					
36175622	0	0	theme	BEP	101:103	arg1	Methods					105:111	Glycoblotting and BEP Methods	83:111	Glycoblotting and BEP Methods	83:111	Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods.					
36175622	3	1	theme	cell	524:527	arg1	growth					529:534	cell growth	524:534	cell growth	524:534	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	2	2	theme	free	354:357	arg1	oligosaccharides					359:374	free oligosaccharides	354:374	free oligosaccharides	354:374	Glycans are covalently attached to proteins and lipids, and are classified into subclasses such as N-linked glycans, glycosaminoglycans, glycosphingolipid-glycans, free oligosaccharides, and O-linked glycans according to their biosynthetic pathways.					
36175622	2	3	attach	attached	213:220	arg1	proteins					225:232	proteins	225:232	proteins	225:232	Glycans are covalently attached to proteins and lipids, and are classified into subclasses such as N-linked glycans, glycosaminoglycans, glycosphingolipid-glycans, free oligosaccharides, and O-linked glycans according to their biosynthetic pathways.					
36175622	2	3	attach	attached	213:220	arg2	Glycans					190:196	Glycans	190:196	Glycans	190:196	Glycans are covalently attached to proteins and lipids, and are classified into subclasses such as N-linked glycans, glycosaminoglycans, glycosphingolipid-glycans, free oligosaccharides, and O-linked glycans according to their biosynthetic pathways.					
36175622	2	3	attach	attached	213:220	arg1	lipids					238:243	lipids	238:243	lipids	238:243	Glycans are covalently attached to proteins and lipids, and are classified into subclasses such as N-linked glycans, glycosaminoglycans, glycosphingolipid-glycans, free oligosaccharides, and O-linked glycans according to their biosynthetic pathways.					
36175622	5	4	theme	comprehensive	741:753	arg1	analysis					773:780	comprehensive cellular glycomic analysis	741:780	comprehensive cellular glycomic analysis using glycoblotting and β-elimination with pyrazolone methods	741:842	In this chapter, we describe detailed protocols to prepare glycans, and perform comprehensive cellular glycomic analysis using glycoblotting and β-elimination with pyrazolone methods.					
36175622	4	5	theme	attack	642:647	arg1	cells					654:658	and attack host cells	638:658	cells	654:658	During infection, bacteria and viruses often use glycans to recognize and attack host cells.					
36175622	5	6	theme	cellular	755:762	arg1	analysis					773:780	comprehensive cellular glycomic analysis	741:780	comprehensive cellular glycomic analysis using glycoblotting and β-elimination with pyrazolone methods	741:842	In this chapter, we describe detailed protocols to prepare glycans, and perform comprehensive cellular glycomic analysis using glycoblotting and β-elimination with pyrazolone methods.					
36175622	2	7	theme	biosynthetic	417:428	arg1	pathways					430:437	their biosynthetic pathways	411:437	their biosynthetic pathways	411:437	Glycans are covalently attached to proteins and lipids, and are classified into subclasses such as N-linked glycans, glycosaminoglycans, glycosphingolipid-glycans, free oligosaccharides, and O-linked glycans according to their biosynthetic pathways.					
36175622	3	8	theme	complex	446:452	arg1	glycans					454:460	These complex glycans	440:460	These complex glycans	440:460	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	5	9	with	β-elimination	806:818	arg1	methods					836:842	pyrazolone methods	825:842	pyrazolone methods	825:842	In this chapter, we describe detailed protocols to prepare glycans, and perform comprehensive cellular glycomic analysis using glycoblotting and β-elimination with pyrazolone methods.					
36175622	0	10	theme	Cellular	14:21	arg1	Profiling					30:38	Comprehensive Cellular Glycan Profiling	0:38	Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods	0:111	Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods.					
36175622	5	11	theme	glycomic	764:771	arg1	analysis					773:780	comprehensive cellular glycomic analysis	741:780	comprehensive cellular glycomic analysis using glycoblotting and β-elimination with pyrazolone methods	741:842	In this chapter, we describe detailed protocols to prepare glycans, and perform comprehensive cellular glycomic analysis using glycoblotting and β-elimination with pyrazolone methods.					
36175622	0	12	theme	Comprehensive	0:12	arg1	Profiling					30:38	Comprehensive Cellular Glycan Profiling	0:38	Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods	0:111	Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods.					
36175622	2	13	theme	O-linked	381:388	arg1	glycans					390:396	O-linked glycans	381:396	O-linked glycans	381:396	Glycans are covalently attached to proteins and lipids, and are classified into subclasses such as N-linked glycans, glycosaminoglycans, glycosphingolipid-glycans, free oligosaccharides, and O-linked glycans according to their biosynthetic pathways.					
36175622	2	14	theme	N-linked	289:296	arg1	glycans					298:304	N-linked glycans	289:304	N-linked glycans	289:304	Glycans are covalently attached to proteins and lipids, and are classified into subclasses such as N-linked glycans, glycosaminoglycans, glycosphingolipid-glycans, free oligosaccharides, and O-linked glycans according to their biosynthetic pathways.					
36175622	4	15	theme	host	649:652	arg1	cells					654:658	and attack host cells	638:658	cells	654:658	During infection, bacteria and viruses often use glycans to recognize and attack host cells.					
36175622	0	16	theme	Glycan	23:28	arg1	Profiling					30:38	Comprehensive Cellular Glycan Profiling	0:38	Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods	0:111	Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods.					
36175622	3	17	theme	pathological	492:503	arg1	adhesion					558:565	adhesion	558:565	adhesion	558:565	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	3	17	theme	pathological	492:503	arg1	growth					529:534	cell growth	524:534	cell growth	524:534	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	3	17	theme	pathological	492:503	arg1	processes					505:513	various biological and pathological processes	469:513	various biological and pathological processes	469:513	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	3	17	theme	pathological	492:503	arg1	differentiation					537:551	differentiation	537:551	differentiation	537:551	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	0	18	theme	Glycoproteins	43:55	arg1	Profiling					30:38	Comprehensive Cellular Glycan Profiling	0:38	Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods	0:111	Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods.					
36175622	2	19	link	O-linked	381:388	arg1	glycans					390:396	O-linked glycans	381:396	O-linked glycans	381:396	Glycans are covalently attached to proteins and lipids, and are classified into subclasses such as N-linked glycans, glycosaminoglycans, glycosphingolipid-glycans, free oligosaccharides, and O-linked glycans according to their biosynthetic pathways.					
36175622	1	20	theme	cell	184:187	arg1	surface					167:173	the surface	163:173	the surface of every cell	163:187	The glycocalyx is a layer of glycans that covers the surface of every cell.					
36175622	3	21	theme	various	469:475	arg1	adhesion					558:565	adhesion	558:565	adhesion	558:565	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	3	21	theme	various	469:475	arg1	growth					529:534	cell growth	524:534	cell growth	524:534	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	3	21	theme	various	469:475	arg1	processes					505:513	various biological and pathological processes	469:513	various biological and pathological processes	469:513	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	3	21	theme	various	469:475	arg1	differentiation					537:551	differentiation	537:551	differentiation	537:551	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	2	22	link	N-linked	289:296	arg1	glycans					298:304	N-linked glycans	289:304	N-linked glycans	289:304	Glycans are covalently attached to proteins and lipids, and are classified into subclasses such as N-linked glycans, glycosaminoglycans, glycosphingolipid-glycans, free oligosaccharides, and O-linked glycans according to their biosynthetic pathways.					
36175622	3	23	theme	biological	477:486	arg1	adhesion					558:565	adhesion	558:565	adhesion	558:565	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	3	23	theme	biological	477:486	arg1	growth					529:534	cell growth	524:534	cell growth	524:534	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	3	23	theme	biological	477:486	arg1	processes					505:513	various biological and pathological processes	469:513	various biological and pathological processes	469:513	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	3	23	theme	biological	477:486	arg1	differentiation					537:551	differentiation	537:551	differentiation	537:551	These complex glycans affect various biological and pathological processes, such as cell growth, differentiation, and adhesion.					
36175622	0	24	theme	Glycosphingolipids	61:78	arg1	Profiling					30:38	Comprehensive Cellular Glycan Profiling	0:38	Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods	0:111	Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods.					
36175622	5	25	theme	pyrazolone	825:834	arg1	methods					836:842	pyrazolone methods	825:842	pyrazolone methods	825:842	In this chapter, we describe detailed protocols to prepare glycans, and perform comprehensive cellular glycomic analysis using glycoblotting and β-elimination with pyrazolone methods.					
36175622	5	26	theme	detailed	690:697	arg1	protocols					699:707	detailed protocols	690:707	detailed protocols	690:707	In this chapter, we describe detailed protocols to prepare glycans, and perform comprehensive cellular glycomic analysis using glycoblotting and β-elimination with pyrazolone methods.					
36175622	5	27	with	glycoblotting	788:800	arg1	methods					836:842	pyrazolone methods	825:842	pyrazolone methods	825:842	In this chapter, we describe detailed protocols to prepare glycans, and perform comprehensive cellular glycomic analysis using glycoblotting and β-elimination with pyrazolone methods.					
36175622	0	28	theme	Glycoblotting	83:95	arg1	Methods					105:111	Glycoblotting and BEP Methods	83:111	Glycoblotting and BEP Methods	83:111	Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods.					
36175622	1	29	theme	glycans	143:149	arg1	layer					134:138	a layer	132:138	a layer of glycans that covers the surface of every cell	132:187	The glycocalyx is a layer of glycans that covers the surface of every cell.					
36175622	1	29	theme	glycans	143:149	arg1	glycocalyx					118:127	The glycocalyx	114:127	The glycocalyx	114:127	The glycocalyx is a layer of glycans that covers the surface of every cell.					
36604055	6	0	theme	P	865:865	arg1	/CS/MOF-808					876:886	The P(AM-co-AA)/CS/MOF-808	861:886	The P(AM-co-AA)/CS/MOF-808	861:886	The P(AM-co-AA)/CS/MOF-808 also exhibits good repeatability and stability after five adsorption-desorption cycles.					
36604055	7	1	theme	uranium	980:986	arg1	capacity					999:1006	The uranium adsorption capacity	976:1006	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater	976:1069	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	7	1	theme	uranium	980:986	arg1	6.2 mg g-1					1074:1083	6.2 mg g-1	1074:1083	6.2 mg g-1	1074:1083	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	2	2	from	5 h	476:478	arg1	water					496:500	water	496:500	water	496:500	The CS chain, which contains NH2, reduces the swelling ratio of the hydrogel to 4.17 after 5 h of immersion in water.					
36604055	0	3	theme	uranium	78:84	arg1	extraction					86:95	uranium extraction	78:95	uranium extraction from seawater	78:109	MOF-implanted poly (acrylamide-co-acrylic acid)/chitosan organic hydrogel for uranium extraction from seawater.					
36604055	3	4	theme	P	576:576	arg1	/CS					587:589	P(AM-co-AA)/CS	576:589	P(AM-co-AA)/CS	576:589	The coordinate bond between the MOF and carboxyl group on the surface of P(AM-co-AA)/CS improves the mechanical properties and stability of P(AM-co-AA)/CS.					
36604055	4	5	theme	AM-co-AA	694:701	arg1	capacity					680:687	The U(VI) adsorption capacity	659:687	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808	659:713	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808 is 159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0.					
36604055	4	5	theme	AM-co-AA	694:701	arg1	159.56 mg g-1					718:730	159.56 mg g-1	718:730	159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0	718:764	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808 is 159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0.					
36604055	7	6	from	rate	1094:1097	arg1	hydrogel					1128:1135	the hydrogel	1124:1135	the hydrogel	1124:1135	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	4	7	from	pH = 8.0	757:764	arg1	159.56 mg g-1					718:730	159.56 mg g-1	718:730	159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0	718:764	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808 is 159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0.					
36604055	4	7	from	pH = 8.0	757:764	arg1	capacity					680:687	The U(VI) adsorption capacity	659:687	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808	659:713	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808 is 159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0.					
36604055	7	8	theme	adsorbent	1025:1033	arg1	capacity					999:1006	The uranium adsorption capacity	976:1006	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater	976:1069	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	7	8	theme	adsorbent	1025:1033	arg1	6.2 mg g-1					1074:1083	6.2 mg g-1	1074:1083	6.2 mg g-1	1074:1083	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	1	9	theme	adsorption	224:233	arg1	capacity					235:242	good U (VI) adsorption capacity	212:242	good U (VI) adsorption capacity	212:242	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	1	10	with	hydrogel	139:146	arg1	ratio					168:172	a low swelling ratio	153:172	a low swelling ratio	153:172	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	1	10	with	hydrogel	139:146	arg1	properties					196:205	excellent mechanical properties	175:205	excellent mechanical properties	175:205	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	1	10	with	hydrogel	139:146	arg1	capacity					235:242	good U (VI) adsorption capacity	212:242	good U (VI) adsorption capacity	212:242	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	2	11	theme	swelling	431:438	arg1	ratio					440:444	the swelling ratio	427:444	the swelling ratio of the hydrogel to 4.17	427:468	The CS chain, which contains NH2, reduces the swelling ratio of the hydrogel to 4.17 after 5 h of immersion in water.					
36604055	3	12	theme	mechanical	604:613	arg1	properties					615:624	mechanical properties	604:624	mechanical properties	604:624	The coordinate bond between the MOF and carboxyl group on the surface of P(AM-co-AA)/CS improves the mechanical properties and stability of P(AM-co-AA)/CS.					
36604055	7	13	from	adsorption	1110:1119	arg1	hydrogel					1128:1135	the hydrogel	1124:1135	the hydrogel	1124:1135	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	5	14	theme	Langmuir	812:819	arg1	isotherm					821:828	Langmuir isotherm	812:828	Langmuir isotherm	812:828	The adsorption process is well fitted by the Langmuir isotherm and pseudo-second-order model.					
36604055	3	15	from	bond	518:521	arg1	surface					565:571	the surface	561:571	the surface of P(AM-co-AA)/CS	561:589	The coordinate bond between the MOF and carboxyl group on the surface of P(AM-co-AA)/CS improves the mechanical properties and stability of P(AM-co-AA)/CS.					
36604055	1	16	theme	low	155:157	arg1	ratio					168:172	a low swelling ratio	153:172	a low swelling ratio	153:172	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	7	17	theme	natural	1054:1060	arg1	seawater					1062:1069	natural seawater	1054:1069	natural seawater	1054:1069	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	1	18	theme	acrylamide-co-acrylic	320:340	arg1	acid					342:345	acrylamide-co-acrylic acid	320:345	acrylamide-co-acrylic acid	320:345	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	1	19	theme	swelling	159:166	arg1	ratio					168:172	a low swelling ratio	153:172	a low swelling ratio	153:172	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	7	20	from	month	1045:1049	arg1	seawater					1062:1069	natural seawater	1054:1069	natural seawater	1054:1069	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	7	21	theme	uranium	1102:1108	arg1	adsorption					1110:1119	uranium adsorption	1102:1119	uranium adsorption on the hydrogel	1102:1135	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	0	22	theme	MOF-implanted	0:12	arg1	poly					14:17	MOF-implanted poly	0:17	MOF-implanted poly (acrylamide-co-acrylic acid)	0:46	MOF-implanted poly (acrylamide-co-acrylic acid)/chitosan organic hydrogel for uranium extraction from seawater.					
36604055	0	22	theme	MOF-implanted	0:12	arg1	acid					42:45	acrylamide-co-acrylic acid	20:45	acrylamide-co-acrylic acid	20:45	MOF-implanted poly (acrylamide-co-acrylic acid)/chitosan organic hydrogel for uranium extraction from seawater.					
36604055	4	23	theme	P	692:692	arg1	AM-co-AA					694:701	P(AM-co-AA)/CS/MOF-808	692:713	P(AM-co-AA)/CS/MOF-808	692:713	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808 is 159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0.					
36604055	7	24	from	hydrogel	1128:1135	arg1	rate					1094:1097	the rate	1090:1097	the rate of uranium adsorption on the hydrogel	1090:1135	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	7	24	from	hydrogel	1128:1135	arg1	0.21 mg g-1 day-1					1140:1156	0.21 mg g-1 day-1	1140:1156	0.21 mg g-1 day-1	1140:1156	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	6	25	theme	good	902:905	arg1	repeatability					907:919	good repeatability	902:919	good repeatability	902:919	The P(AM-co-AA)/CS/MOF-808 also exhibits good repeatability and stability after five adsorption-desorption cycles.					
36604055	0	26	theme	acrylamide-co-acrylic	20:40	arg1	poly					14:17	MOF-implanted poly	0:17	MOF-implanted poly (acrylamide-co-acrylic acid)	0:46	MOF-implanted poly (acrylamide-co-acrylic acid)/chitosan organic hydrogel for uranium extraction from seawater.					
36604055	0	26	theme	acrylamide-co-acrylic	20:40	arg1	acid					42:45	acrylamide-co-acrylic acid	20:45	acrylamide-co-acrylic acid	20:45	MOF-implanted poly (acrylamide-co-acrylic acid)/chitosan organic hydrogel for uranium extraction from seawater.					
36604055	4	27	theme	U	663:663	arg1	capacity					680:687	The U(VI) adsorption capacity	659:687	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808	659:713	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808 is 159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0.					
36604055	4	27	theme	U	663:663	arg1	159.56 mg g-1					718:730	159.56 mg g-1	718:730	159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0	718:764	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808 is 159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0.					
36604055	4	28	from	C0 = 99.47 mg L-1	735:751	arg1	159.56 mg g-1					718:730	159.56 mg g-1	718:730	159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0	718:764	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808 is 159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0.					
36604055	4	28	from	C0 = 99.47 mg L-1	735:751	arg1	capacity					680:687	The U(VI) adsorption capacity	659:687	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808	659:713	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808 is 159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0.					
36604055	1	29	theme	excellent	175:183	arg1	properties					196:205	excellent mechanical properties	175:205	excellent mechanical properties	175:205	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	4	30	dep	capacity	680:687	arg1	VI					665:666	VI	665:666	VI	665:666	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808 is 159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0.					
36604055	3	31	theme	carboxyl	543:550	arg1	group					552:556	the MOF and carboxyl group	531:556	group	552:556	The coordinate bond between the MOF and carboxyl group on the surface of P(AM-co-AA)/CS improves the mechanical properties and stability of P(AM-co-AA)/CS.					
36604055	1	32	theme	mechanical	185:194	arg1	properties					196:205	excellent mechanical properties	175:205	excellent mechanical properties	175:205	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	2	33	theme	hydrogel	453:460	arg1	ratio					440:444	the swelling ratio	427:444	the swelling ratio of the hydrogel to 4.17	427:468	The CS chain, which contains NH2, reduces the swelling ratio of the hydrogel to 4.17 after 5 h of immersion in water.					
36604055	1	34	theme	/chitosan	347:355	arg1	composite					374:382	a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite	312:382	a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite	312:382	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	3	35	theme	MOF	535:537	arg1	group					552:556	the MOF and carboxyl group	531:556	group	552:556	The coordinate bond between the MOF and carboxyl group on the surface of P(AM-co-AA)/CS improves the mechanical properties and stability of P(AM-co-AA)/CS.					
36604055	3	36	theme	/CS	654:656	arg1	stability					630:638	stability	630:638	stability	630:638	The coordinate bond between the MOF and carboxyl group on the surface of P(AM-co-AA)/CS improves the mechanical properties and stability of P(AM-co-AA)/CS.					
36604055	3	36	theme	/CS	654:656	arg1	properties					615:624	mechanical properties	604:624	mechanical properties	604:624	The coordinate bond between the MOF and carboxyl group on the surface of P(AM-co-AA)/CS improves the mechanical properties and stability of P(AM-co-AA)/CS.					
36604055	2	37	contain	contains	405:412	arg1	chain					392:396	The CS chain	385:396	The CS chain	385:396	The CS chain, which contains NH2, reduces the swelling ratio of the hydrogel to 4.17 after 5 h of immersion in water.					
36604055	2	37	contain	contains	405:412	arg2	NH2					414:416	NH2	414:416	NH2	414:416	The CS chain, which contains NH2, reduces the swelling ratio of the hydrogel to 4.17 after 5 h of immersion in water.					
36604055	0	38	theme	organic	57:63	arg1	hydrogel					65:72	organic hydrogel	57:72	organic hydrogel for uranium extraction from seawater	57:109	MOF-implanted poly (acrylamide-co-acrylic acid)/chitosan organic hydrogel for uranium extraction from seawater.					
36604055	1	39	theme	metal-organic	277:289	arg1	MOF					302:304	MOF	302:304	MOF	302:304	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	1	39	theme	metal-organic	277:289	arg1	framework					291:299	a metal-organic framework	275:299	a metal-organic framework (MOF)	275:305	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	2	40	theme	immersion	483:491	arg1	5 h					476:478	5 h	476:478	5 h of immersion in water	476:500	The CS chain, which contains NH2, reduces the swelling ratio of the hydrogel to 4.17 after 5 h of immersion in water.					
36604055	1	41	theme	P	358:358	arg1	/CS					369:371	P(AM-co-AA)/CS	358:371	P(AM-co-AA)/CS	358:371	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	4	42	theme	adsorption	669:678	arg1	capacity					680:687	The U(VI) adsorption capacity	659:687	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808	659:713	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808 is 159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0.					
36604055	4	42	theme	adsorption	669:678	arg1	159.56 mg g-1					718:730	159.56 mg g-1	718:730	159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0	718:764	The U(VI) adsorption capacity of P(AM-co-AA)/CS/MOF-808 is 159.56 mg g-1 at C0 = 99.47 mg L-1 and pH = 8.0.					
36604055	3	43	theme	/CS	587:589	arg1	surface					565:571	the surface	561:571	the surface of P(AM-co-AA)/CS	561:589	The coordinate bond between the MOF and carboxyl group on the surface of P(AM-co-AA)/CS improves the mechanical properties and stability of P(AM-co-AA)/CS.					
36604055	2	44	theme	CS	389:390	arg1	chain					392:396	The CS chain	385:396	The CS chain	385:396	The CS chain, which contains NH2, reduces the swelling ratio of the hydrogel to 4.17 after 5 h of immersion in water.					
36604055	3	45	theme	P	643:643	arg1	/CS					654:656	P(AM-co-AA)/CS	643:656	P(AM-co-AA)/CS	643:656	The coordinate bond between the MOF and carboxyl group on the surface of P(AM-co-AA)/CS improves the mechanical properties and stability of P(AM-co-AA)/CS.					
36604055	1	46	dep	capacity	235:242	arg1	VI					220:221	VI	220:221	VI	220:221	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	5	47	dep	isotherm	821:828	arg1	the					808:810	the	808:810	the	808:810	The adsorption process is well fitted by the Langmuir isotherm and pseudo-second-order model.					
36604055	7	48	theme	adsorption	988:997	arg1	capacity					999:1006	The uranium adsorption capacity	976:1006	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater	976:1069	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	7	48	theme	adsorption	988:997	arg1	6.2 mg g-1					1074:1083	6.2 mg g-1	1074:1083	6.2 mg g-1	1074:1083	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	7	49	theme	adsorption	1110:1119	arg1	rate					1094:1097	the rate	1090:1097	the rate of uranium adsorption on the hydrogel	1090:1135	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	7	49	theme	adsorption	1110:1119	arg1	0.21 mg g-1 day-1					1140:1156	0.21 mg g-1 day-1	1140:1156	0.21 mg g-1 day-1	1140:1156	The uranium adsorption capacity of the developed adsorbent after one month in natural seawater is 6.2 mg g-1, and the rate of uranium adsorption on the hydrogel is 0.21 mg g-1 day-1.					
36604055	1	50	theme	AM-co-AA	360:367	arg1	/CS					369:371	P(AM-co-AA)/CS	358:371	P(AM-co-AA)/CS	358:371	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	6	51	theme	AM-co-AA	867:874	arg1	/CS/MOF-808					876:886	The P(AM-co-AA)/CS/MOF-808	861:886	The P(AM-co-AA)/CS/MOF-808	861:886	The P(AM-co-AA)/CS/MOF-808 also exhibits good repeatability and stability after five adsorption-desorption cycles.					
36604055	0	52	from	seawater	102:109	arg1	extraction					86:95	uranium extraction	78:95	uranium extraction from seawater	78:109	MOF-implanted poly (acrylamide-co-acrylic acid)/chitosan organic hydrogel for uranium extraction from seawater.					
36604055	5	53	theme	pseudo-second-order	834:852	arg1	model					854:858	pseudo-second-order model	834:858	pseudo-second-order model	834:858	The adsorption process is well fitted by the Langmuir isotherm and pseudo-second-order model.					
36604055	5	54	theme	adsorption	771:780	arg1	process					782:788	The adsorption process	767:788	The adsorption process	767:788	The adsorption process is well fitted by the Langmuir isotherm and pseudo-second-order model.					
36604055	1	55	theme	good	212:215	arg1	capacity					235:242	good U (VI) adsorption capacity	212:242	good U (VI) adsorption capacity	212:242	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	3	56	theme	coordinate	507:516	arg1	bond					518:521	The coordinate bond	503:521	The coordinate bond between the MOF and carboxyl group on the surface of P(AM-co-AA)/CS	503:589	The coordinate bond between the MOF and carboxyl group on the surface of P(AM-co-AA)/CS improves the mechanical properties and stability of P(AM-co-AA)/CS.					
36604055	1	57	theme	composite	129:137	arg1	hydrogel					139:146	a composite hydrogel	127:146	a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity	127:242	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	1	58	theme	U	217:217	arg1	capacity					235:242	good U (VI) adsorption capacity	212:242	good U (VI) adsorption capacity	212:242	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	3	59	dep	properties	615:624	arg1	the					600:602	the	600:602	the	600:602	The coordinate bond between the MOF and carboxyl group on the surface of P(AM-co-AA)/CS improves the mechanical properties and stability of P(AM-co-AA)/CS.					
36604055	6	60	theme	adsorption-desorption	946:966	arg1	cycles					968:973	five adsorption-desorption cycles	941:973	five adsorption-desorption cycles	941:973	The P(AM-co-AA)/CS/MOF-808 also exhibits good repeatability and stability after five adsorption-desorption cycles.					
36604055	1	61	dep	/chitosan	347:355	arg1	acid					342:345	acrylamide-co-acrylic acid	320:345	acrylamide-co-acrylic acid	320:345	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36604055	1	61	dep	/chitosan	347:355	arg1	/CS					369:371	P(AM-co-AA)/CS	358:371	P(AM-co-AA)/CS	358:371	In this study, a composite hydrogel with a low swelling ratio, excellent mechanical properties, and good U (VI) adsorption capacity was developed by incorporating a metal-organic framework (MOF) with a poly (acrylamide-co-acrylic acid)/chitosan (P(AM-co-AA)/CS) composite.					
36694095	0	0	theme	flour	80:84	arg1	characteristics					36:50	quality characteristics	28:50	quality characteristics of stone-milled whole wheat flour	28:84	Effects of particle size on quality characteristics of stone-milled whole wheat flour.					
36694095	5	1	theme	softening	666:674	arg1	absorption					644:653	The water absorption	634:653	The water absorption	634:653	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	1	theme	softening	666:674	arg1	solubility					698:707	solubility	698:707	solubility	698:707	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	1	theme	softening	666:674	arg1	syneresis					714:722	syneresis	714:722	syneresis	714:722	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	1	theme	softening	666:674	arg1	temperature					685:695	pasting temperature	677:695	pasting temperature	677:695	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	1	theme	softening	666:674	arg1	degree					656:661	degree	656:661	degree of softening	656:674	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	0	2	theme	wheat	74:78	arg1	flour					80:84	stone-milled whole wheat flour	55:84	stone-milled whole wheat flour	55:84	Effects of particle size on quality characteristics of stone-milled whole wheat flour.					
36694095	1	3	theme	direct	138:143	arg1	method					154:159	the direct crushing method	134:159	the direct crushing method	134:159	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method preserves all the components of the whole wheat grain.					
36694095	0	4	from	Effects	0:6	arg1	characteristics					36:50	quality characteristics	28:50	quality characteristics of stone-milled whole wheat flour	28:84	Effects of particle size on quality characteristics of stone-milled whole wheat flour.					
36694095	8	5	theme	particle	1199:1206	arg1	size					1208:1211	The particle size	1195:1211	The particle size	1195:1211	CONCLUSION The particle size exerted influence on the quality characteristics of stone-milled WWF, and the overall properties of WWF were better at medium particle size.					
36694095	3	6	theme	functional	454:463	arg1	properties					465:474	functional properties	454:474	functional properties	454:474	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	7	theme	starch	477:482	arg1	microstructure					484:497	starch microstructure	477:497	starch microstructure	477:497	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	5	8	theme	water	638:642	arg1	absorption					644:653	The water absorption	634:653	The water absorption	634:653	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	6	9	theme	starch	1036:1041	arg1	granules					1043:1050	starch granules	1036:1050	starch granules	1036:1050	The scanning electron microscope micrographs revealed that the larger the particle size of WWF, the denser the distribution of starch granules.					
36694095	5	10	theme	holding	849:855	arg1	capacity					857:864	water holding capacity	843:864	water holding capacity	843:864	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	4	11	dep	higher	599:604	arg1	content					625:631	the damaged starch content	606:631	the higher the damaged starch content	595:631	RESULTS The smaller the particle size of WWF, the higher the damaged starch content.					
36694095	7	12	theme	β-turn	1069:1074	arg1	highest					1127:1133	highest	1127:1133	highest	1127:1133	The β-sheet and β-turn contents of WWF with particle size 180 μm were the highest, reaching up to 33.85% and 39.79%, respectively.					
36694095	7	12	theme	β-turn	1069:1074	arg1	contents					1076:1083	The β-sheet and β-turn contents	1053:1083	The β-sheet and β-turn contents of WWF with particle size 180 μm	1053:1116	The β-sheet and β-turn contents of WWF with particle size 180 μm were the highest, reaching up to 33.85% and 39.79%, respectively.					
36694095	5	13	theme	final	810:814	arg1	viscosity					816:824	final viscosity	810:824	final viscosity	810:824	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	1	14	theme	crushing	145:152	arg1	method					154:159	the direct crushing method	134:159	the direct crushing method	134:159	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method preserves all the components of the whole wheat grain.					
36694095	5	15	theme	peak	794:797	arg1	viscosity					799:807	the peak viscosity	790:807	the peak viscosity	790:807	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	16	theme	pasting	677:683	arg1	temperature					685:695	pasting temperature	677:695	pasting temperature	677:695	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	7	17	dep	33.85	1151:1155	arg1	to					1148:1149	to	1148:1149	to	1148:1149	The β-sheet and β-turn contents of WWF with particle size 180 μm were the highest, reaching up to 33.85% and 39.79%, respectively.					
36694095	6	18	theme	particle	983:990	arg1	size					992:995	the particle size	979:995	the larger the particle size of WWF	968:1002	The scanning electron microscope micrographs revealed that the larger the particle size of WWF, the denser the distribution of starch granules.					
36694095	4	19	theme	particle	573:580	arg1	size					582:585	the particle size	569:585	The smaller the particle size of WWF	557:592	RESULTS The smaller the particle size of WWF, the higher the damaged starch content.					
36694095	7	20	theme	β-sheet	1057:1063	arg1	highest					1127:1133	highest	1127:1133	highest	1127:1133	The β-sheet and β-turn contents of WWF with particle size 180 μm were the highest, reaching up to 33.85% and 39.79%, respectively.					
36694095	7	20	theme	β-sheet	1057:1063	arg1	contents					1076:1083	The β-sheet and β-turn contents	1053:1083	The β-sheet and β-turn contents of WWF with particle size 180 μm	1053:1116	The β-sheet and β-turn contents of WWF with particle size 180 μm were the highest, reaching up to 33.85% and 39.79%, respectively.					
36694095	7	21	theme	particle	1097:1104	arg1	size					1106:1109	particle size 180 μm	1097:1116	particle size 180 μm	1097:1116	The β-sheet and β-turn contents of WWF with particle size 180 μm were the highest, reaching up to 33.85% and 39.79%, respectively.					
36694095	6	22	theme	granules	1043:1050	arg1	distribution					1020:1031	the distribution	1016:1031	the distribution of starch granules	1016:1050	The scanning electron microscope micrographs revealed that the larger the particle size of WWF, the denser the distribution of starch granules.					
36694095	2	23	theme	stone	308:312	arg1	milling					314:320	stone milling	308:320	stone milling	308:320	WWF with different particle sizes (180, 150, 125, 106, and 96 μm) was obtained by combining stone milling and particle size sieving technology.					
36694095	8	24	theme	medium	1332:1337	arg1	size					1348:1351	medium particle size	1332:1351	medium particle size	1332:1351	CONCLUSION The particle size exerted influence on the quality characteristics of stone-milled WWF, and the overall properties of WWF were better at medium particle size.					
36694095	1	25	theme	BACKGROUND	87:96	arg1	WWF					117:119	WWF	117:119	WWF	117:119	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method preserves all the components of the whole wheat grain.					
36694095	1	25	theme	BACKGROUND	87:96	arg1	flour					110:114	BACKGROUND Whole wheat flour	87:114	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method	87:159	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method preserves all the components of the whole wheat grain.					
36694095	0	26	theme	size	20:23	arg1	Effects					0:6	Effects	0:6	Effects of particle size on quality characteristics of stone-milled whole wheat flour	0:84	Effects of particle size on quality characteristics of stone-milled whole wheat flour.					
36694095	8	27	theme	overall	1291:1297	arg1	properties					1299:1308	the overall properties	1287:1308	the overall properties of WWF	1287:1315	CONCLUSION The particle size exerted influence on the quality characteristics of stone-milled WWF, and the overall properties of WWF were better at medium particle size.					
36694095	7	28	with	WWF	1088:1090	arg1	size					1106:1109	particle size 180 μm	1097:1116	particle size 180 μm	1097:1116	The β-sheet and β-turn contents of WWF with particle size 180 μm were the highest, reaching up to 33.85% and 39.79%, respectively.					
36694095	1	29	theme	Whole	98:102	arg1	WWF					117:119	WWF	117:119	WWF	117:119	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method preserves all the components of the whole wheat grain.					
36694095	1	29	theme	Whole	98:102	arg1	flour					110:114	BACKGROUND Whole wheat flour	87:114	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method	87:159	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method preserves all the components of the whole wheat grain.					
36694095	5	30	theme	water	843:847	arg1	capacity					857:864	water holding capacity	843:864	water holding capacity	843:864	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	0	31	theme	particle	11:18	arg1	size					20:23	particle size	11:23	particle size	11:23	Effects of particle size on quality characteristics of stone-milled whole wheat flour.					
36694095	5	32	theme	swelling	827:834	arg1	power					836:840	swelling power	827:840	swelling power	827:840	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	1	33	theme	wheat	104:108	arg1	WWF					117:119	WWF	117:119	WWF	117:119	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method preserves all the components of the whole wheat grain.					
36694095	1	33	theme	wheat	104:108	arg1	flour					110:114	BACKGROUND Whole wheat flour	87:114	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method	87:159	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method preserves all the components of the whole wheat grain.					
36694095	2	34	theme	sieving	340:346	arg1	technology					348:357	sieving technology	340:357	sieving technology	340:357	WWF with different particle sizes (180, 150, 125, 106, and 96 μm) was obtained by combining stone milling and particle size sieving technology.					
36694095	0	35	theme	quality	28:34	arg1	characteristics					36:50	quality characteristics	28:50	quality characteristics of stone-milled whole wheat flour	28:84	Effects of particle size on quality characteristics of stone-milled whole wheat flour.					
36694095	8	36	from	influence	1221:1229	arg1	characteristics					1246:1260	the quality characteristics	1234:1260	the quality characteristics of stone-milled WWF	1234:1280	CONCLUSION The particle size exerted influence on the quality characteristics of stone-milled WWF, and the overall properties of WWF were better at medium particle size.					
36694095	6	37	theme	microscope	931:940	arg1	micrographs					942:952	The scanning electron microscope micrographs	909:952	The scanning electron microscope micrographs	909:952	The scanning electron microscope micrographs revealed that the larger the particle size of WWF, the denser the distribution of starch granules.					
36694095	8	38	dep	CONCLUSION	1184:1193	arg1	size					1348:1351	medium particle size	1332:1351	medium particle size	1332:1351	CONCLUSION The particle size exerted influence on the quality characteristics of stone-milled WWF, and the overall properties of WWF were better at medium particle size.					
36694095	8	38	dep	CONCLUSION	1184:1193	arg1	exerted					1213:1219	exerted	1213:1219	exerted influence on the quality characteristics of stone-milled WWF	1213:1280	CONCLUSION The particle size exerted influence on the quality characteristics of stone-milled WWF, and the overall properties of WWF were better at medium particle size.					
36694095	3	39	theme	particle	375:382	arg1	size					384:387	particle size	375:387	particle size	375:387	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	6	40	theme	scanning	913:920	arg1	micrographs					942:952	The scanning electron microscope micrographs	909:952	The scanning electron microscope micrographs	909:952	The scanning electron microscope micrographs revealed that the larger the particle size of WWF, the denser the distribution of starch granules.					
36694095	3	41	theme	stone-milled	513:524	arg1	WWF					526:528	stone-milled WWF	513:528	stone-milled WWF	513:528	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	2	42	theme	particle	326:333	arg1	size					335:338	particle size	326:338	particle size	326:338	WWF with different particle sizes (180, 150, 125, 106, and 96 μm) was obtained by combining stone milling and particle size sieving technology.					
36694095	2	43	with	WWF	216:218	arg1	sizes					244:248	different particle sizes	225:248	different particle sizes (180, 150, 125, 106, and 96 μm)	225:280	WWF with different particle sizes (180, 150, 125, 106, and 96 μm) was obtained by combining stone milling and particle size sieving technology.					
36694095	6	44	theme	electron	922:929	arg1	micrographs					942:952	The scanning electron microscope micrographs	909:952	The scanning electron microscope micrographs	909:952	The scanning electron microscope micrographs revealed that the larger the particle size of WWF, the denser the distribution of starch granules.					
36694095	8	45	theme	WWF	1313:1315	arg1	properties					1299:1308	the overall properties	1287:1308	the overall properties of WWF	1287:1315	CONCLUSION The particle size exerted influence on the quality characteristics of stone-milled WWF, and the overall properties of WWF were better at medium particle size.					
36694095	8	46	theme	WWF	1278:1280	arg1	characteristics					1246:1260	the quality characteristics	1234:1260	the quality characteristics of stone-milled WWF	1234:1280	CONCLUSION The particle size exerted influence on the quality characteristics of stone-milled WWF, and the overall properties of WWF were better at medium particle size.					
36694095	4	47	theme	starch	618:623	arg1	content					625:631	the damaged starch content	606:631	the higher the damaged starch content	595:631	RESULTS The smaller the particle size of WWF, the higher the damaged starch content.					
36694095	6	48	dep	larger	972:977	arg1	size					992:995	the particle size	979:995	the larger the particle size of WWF	968:1002	The scanning electron microscope micrographs revealed that the larger the particle size of WWF, the denser the distribution of starch granules.					
36694095	5	49	dep	increased	731:739	arg1	whereas					782:788	whereas	782:788	whereas	782:788	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	49	dep	increased	731:739	arg1	decreased					771:779	decreased	771:779	increased steadily as the particle size decreased	731:779	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	4	50	theme	damaged	610:616	arg1	content					625:631	the damaged starch content	606:631	the higher the damaged starch content	595:631	RESULTS The smaller the particle size of WWF, the higher the damaged starch content.					
36694095	5	51	theme	gelatinization	883:896	arg1	power					836:840	swelling power	827:840	swelling power	827:840	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	51	theme	gelatinization	883:896	arg1	capacity					857:864	water holding capacity	843:864	water holding capacity	843:864	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	51	theme	gelatinization	883:896	arg1	enthalpy					871:878	enthalpy	871:878	enthalpy	871:878	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	51	theme	gelatinization	883:896	arg1	viscosity					799:807	the peak viscosity	790:807	the peak viscosity	790:807	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	51	theme	gelatinization	883:896	arg1	viscosity					816:824	final viscosity	810:824	final viscosity	810:824	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	2	52	theme	particle	235:242	arg1	sizes					244:248	different particle sizes	225:248	different particle sizes (180, 150, 125, 106, and 96 μm)	225:280	WWF with different particle sizes (180, 150, 125, 106, and 96 μm) was obtained by combining stone milling and particle size sieving technology.					
36694095	4	53	dep	smaller	561:567	arg1	size					582:585	the particle size	569:585	The smaller the particle size of WWF	557:592	RESULTS The smaller the particle size of WWF, the higher the damaged starch content.					
36694095	3	54	theme	proximate	396:404	arg1	composition					406:416	the proximate composition	392:416	the proximate composition	392:416	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	8	55	theme	quality	1238:1244	arg1	characteristics					1246:1260	the quality characteristics	1234:1260	the quality characteristics of stone-milled WWF	1234:1280	CONCLUSION The particle size exerted influence on the quality characteristics of stone-milled WWF, and the overall properties of WWF were better at medium particle size.					
36694095	1	56	theme	whole	197:201	arg1	grain					209:213	the whole wheat grain	193:213	the whole wheat grain	193:213	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method preserves all the components of the whole wheat grain.					
36694095	8	57	theme	particle	1339:1346	arg1	size					1348:1351	medium particle size	1332:1351	medium particle size	1332:1351	CONCLUSION The particle size exerted influence on the quality characteristics of stone-milled WWF, and the overall properties of WWF were better at medium particle size.					
36694095	2	58	theme	different	225:233	arg1	sizes					244:248	different particle sizes	225:248	different particle sizes (180, 150, 125, 106, and 96 μm)	225:280	WWF with different particle sizes (180, 150, 125, 106, and 96 μm) was obtained by combining stone milling and particle size sieving technology.					
36694095	1	59	theme	wheat	203:207	arg1	grain					209:213	the whole wheat grain	193:213	the whole wheat grain	193:213	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method preserves all the components of the whole wheat grain.					
36694095	0	60	theme	whole	68:72	arg1	flour					80:84	stone-milled whole wheat flour	55:84	stone-milled whole wheat flour	55:84	Effects of particle size on quality characteristics of stone-milled whole wheat flour.					
36694095	3	61	theme	size	384:387	arg1	effects					364:370	The effects	360:370	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF	360:528	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	62	theme	WWF	526:528	arg1	pasting					432:438	pasting	432:438	pasting	432:438	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	62	theme	WWF	526:528	arg1	microstructure					484:497	starch microstructure	477:497	starch microstructure	477:497	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	62	theme	WWF	526:528	arg1	composition					406:416	the proximate composition	392:416	the proximate composition	392:416	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	62	theme	WWF	526:528	arg1	farinograph					419:429	farinograph	419:429	farinograph	419:429	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	62	theme	WWF	526:528	arg1	properties					465:474	functional properties	454:474	functional properties	454:474	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	62	theme	WWF	526:528	arg1	FT-IR					504:508	FT-IR	504:508	FT-IR	504:508	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	5	63	theme	WWF	727:729	arg1	absorption					644:653	The water absorption	634:653	The water absorption	634:653	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	63	theme	WWF	727:729	arg1	solubility					698:707	solubility	698:707	solubility	698:707	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	63	theme	WWF	727:729	arg1	syneresis					714:722	syneresis	714:722	syneresis	714:722	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	63	theme	WWF	727:729	arg1	temperature					685:695	pasting temperature	677:695	pasting temperature	677:695	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	5	63	theme	WWF	727:729	arg1	degree					656:661	degree	656:661	degree of softening	656:674	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	8	64	theme	stone-milled	1265:1276	arg1	WWF					1278:1280	stone-milled WWF	1265:1280	stone-milled WWF	1265:1280	CONCLUSION The particle size exerted influence on the quality characteristics of stone-milled WWF, and the overall properties of WWF were better at medium particle size.					
36694095	5	65	theme	particle	757:764	arg1	size					766:769	the particle size	753:769	the particle size	753:769	The water absorption, degree of softening, pasting temperature, solubility, and syneresis of WWF increased steadily as the particle size decreased, whereas the peak viscosity, final viscosity, swelling power, water holding capacity, and enthalpy of gelatinization decreased.					
36694095	1	66	theme	grain	209:213	arg1	components					179:188	all the components	171:188	all the components of the whole wheat grain	171:213	BACKGROUND Whole wheat flour (WWF) prepared by the direct crushing method preserves all the components of the whole wheat grain.					
36694095	0	67	theme	stone-milled	55:66	arg1	flour					80:84	stone-milled whole wheat flour	55:84	stone-milled whole wheat flour	55:84	Effects of particle size on quality characteristics of stone-milled whole wheat flour.					
36694095	2	68	dep	milling	314:320	arg1	technology					348:357	sieving technology	340:357	sieving technology	340:357	WWF with different particle sizes (180, 150, 125, 106, and 96 μm) was obtained by combining stone milling and particle size sieving technology.					
36694095	3	69	from	effects	364:370	arg1	pasting					432:438	pasting	432:438	pasting	432:438	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	69	from	effects	364:370	arg1	thermal					441:447	thermal	441:447	thermal	441:447	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	69	from	effects	364:370	arg1	microstructure					484:497	starch microstructure	477:497	starch microstructure	477:497	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	69	from	effects	364:370	arg1	composition					406:416	the proximate composition	392:416	the proximate composition	392:416	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	69	from	effects	364:370	arg1	farinograph					419:429	farinograph	419:429	farinograph	419:429	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	69	from	effects	364:370	arg1	properties					465:474	functional properties	454:474	functional properties	454:474	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	3	69	from	effects	364:370	arg1	FT-IR					504:508	FT-IR	504:508	FT-IR	504:508	The effects of particle size on the proximate composition, farinograph, pasting, thermal, and functional properties, starch microstructure, and FT-IR of stone-milled WWF were investigated.					
36694095	2	70	dep	sizes	244:248	arg1	106					266:268	106	266:268	106	266:268	WWF with different particle sizes (180, 150, 125, 106, and 96 μm) was obtained by combining stone milling and particle size sieving technology.					
36694095	2	70	dep	sizes	244:248	arg1	96 μm					275:279	96 μm	275:279	96 μm	275:279	WWF with different particle sizes (180, 150, 125, 106, and 96 μm) was obtained by combining stone milling and particle size sieving technology.					
36694095	7	71	theme	WWF	1088:1090	arg1	highest					1127:1133	highest	1127:1133	highest	1127:1133	The β-sheet and β-turn contents of WWF with particle size 180 μm were the highest, reaching up to 33.85% and 39.79%, respectively.					
36694095	7	71	theme	WWF	1088:1090	arg1	contents					1076:1083	The β-sheet and β-turn contents	1053:1083	The β-sheet and β-turn contents of WWF with particle size 180 μm	1053:1116	The β-sheet and β-turn contents of WWF with particle size 180 μm were the highest, reaching up to 33.85% and 39.79%, respectively.					
36880839	1	0	theme	AE/AKP/2-FPBA	348:360	arg1	fiber					384:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber	273:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness	273:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	0	theme	AE/AKP/2-FPBA	348:360	arg1	system					541:546	a drug delivery system	525:546	a drug delivery system	525:546	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	0	1	with	fibers	45:50	arg1	ester					89:93	an in situ crosslinked alginate ester	57:93	an in situ crosslinked alginate ester for hydrophobic drug delivery	57:123	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.					
36880839	1	2	theme	low	151:153	arg1	bioavailability					155:169	low bioavailability	151:169	low bioavailability	151:169	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	0	3	theme	alginate	80:87	arg1	ester					89:93	an in situ crosslinked alginate ester	57:93	an in situ crosslinked alginate ester for hydrophobic drug delivery	57:123	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.					
36880839	4	4	theme	digestive	864:872	arg1	fluid					874:878	the simulated digestive fluid	850:878	the simulated digestive fluid	850:878	AE targeted the release of curcumin by pH stimulation; the release amount in the simulated colonic fluid reached 100%, while the release amount in the simulated digestive fluid was less than 12%.					
36880839	1	5	theme	acid-catalyzed	456:469	arg1	method					515:520	an acid-catalyzed polyol in situ crosslinked phase separation method	453:520	an acid-catalyzed polyol in situ crosslinked phase separation method	453:520	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	6	theme	bioavailability	155:169	arg1	problems					139:146	the problems	135:146	the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes	135:270	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	0	7	theme	hydrophobic	99:109	arg1	delivery					116:123	hydrophobic drug delivery	99:123	hydrophobic drug delivery	99:123	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.					
36880839	1	8	theme	skin-core	363:371	arg1	fiber					384:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber	273:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness	273:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	8	theme	skin-core	363:371	arg1	system					541:546	a drug delivery system	525:546	a drug delivery system	525:546	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	9	theme	polyol	471:476	arg1	method					515:520	an acid-catalyzed polyol in situ crosslinked phase separation method	453:520	an acid-catalyzed polyol in situ crosslinked phase separation method	453:520	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	5	10	theme	glucose	949:955	arg1	stimulation					957:967	glucose stimulation	949:967	glucose stimulation	949:967	2-FPBA controlled the release rate of curcumin by glucose stimulation, which increases with the increase of 2-FPBA content.					
36880839	2	11	dep	mechanism	562:570	arg1	The					549:551	The	549:551	The	549:551	The reaction mechanism and apparent morphology of the fiber were studied.					
36880839	1	12	theme	structural	373:382	arg1	fiber					384:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber	273:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness	273:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	12	theme	structural	373:382	arg1	system					541:546	a drug delivery system	525:546	a drug delivery system	525:546	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	6	13	theme	structural	1084:1093	arg1	fiber					1095:1099	the skin-core structural fiber	1070:1099	the skin-core structural fiber	1070:1099	Moreover, the cytotoxicity test confirmed that the skin-core structural fiber was non-toxic.					
36880839	6	13	theme	structural	1084:1093	arg1	non-toxic					1105:1113	non-toxic	1105:1113	non-toxic	1105:1113	Moreover, the cytotoxicity test confirmed that the skin-core structural fiber was non-toxic.					
36880839	1	14	theme	diabetes	263:270	arg1	treatment					250:258	the treatment	246:258	the treatment of diabetes	246:270	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	2	15	theme	apparent	576:583	arg1	morphology					585:594	apparent morphology	576:594	apparent morphology	576:594	The reaction mechanism and apparent morphology of the fiber were studied.					
36880839	4	16	theme	colonic	794:800	arg1	fluid					802:806	the simulated colonic fluid	780:806	the simulated colonic fluid	780:806	AE targeted the release of curcumin by pH stimulation; the release amount in the simulated colonic fluid reached 100%, while the release amount in the simulated digestive fluid was less than 12%.					
36880839	3	17	from	ability	646:652	arg1	liquids					680:686	simulated liquids	670:686	simulated liquids	670:686	The controlled release ability of the fiber in simulated liquids was evaluated.					
36880839	0	18	theme	drug	111:114	arg1	delivery					116:123	hydrophobic drug delivery	99:123	hydrophobic drug delivery	99:123	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.					
36880839	7	19	theme	skin-core	1143:1151	arg1	fibers					1164:1169	skin-core structural fibers	1143:1169	skin-core structural fibers	1143:1169	These results suggest that skin-core structural fibers have great potential as curcumin delivery systems.					
36880839	1	20	theme	crosslinked	486:496	arg1	method					515:520	an acid-catalyzed polyol in situ crosslinked phase separation method	453:520	an acid-catalyzed polyol in situ crosslinked phase separation method	453:520	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	4	21	theme	simulated	784:792	arg1	fluid					802:806	the simulated colonic fluid	780:806	the simulated colonic fluid	780:806	AE targeted the release of curcumin by pH stimulation; the release amount in the simulated colonic fluid reached 100%, while the release amount in the simulated digestive fluid was less than 12%.					
36880839	6	22	theme	skin-core	1074:1082	arg1	fiber					1095:1099	the skin-core structural fiber	1070:1099	the skin-core structural fiber	1070:1099	Moreover, the cytotoxicity test confirmed that the skin-core structural fiber was non-toxic.					
36880839	6	22	theme	skin-core	1074:1082	arg1	non-toxic					1105:1113	non-toxic	1105:1113	non-toxic	1105:1113	Moreover, the cytotoxicity test confirmed that the skin-core structural fiber was non-toxic.					
36880839	1	23	theme	low	175:177	arg1	efficiency					198:207	low intestinal release efficiency	175:207	low intestinal release efficiency	175:207	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	0	24	theme	responsive	13:22	arg1	fibers					45:50	Dual-stimuli responsive skin-core structural fibers	0:50	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.	0:124	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.					
36880839	4	25	from	amount	840:845	arg1	fluid					874:878	the simulated digestive fluid	850:878	the simulated digestive fluid	850:878	AE targeted the release of curcumin by pH stimulation; the release amount in the simulated colonic fluid reached 100%, while the release amount in the simulated digestive fluid was less than 12%.					
36880839	4	26	theme	simulated	854:862	arg1	fluid					874:878	the simulated digestive fluid	850:878	the simulated digestive fluid	850:878	AE targeted the release of curcumin by pH stimulation; the release amount in the simulated colonic fluid reached 100%, while the release amount in the simulated digestive fluid was less than 12%.					
36880839	1	27	theme	phase	498:502	arg1	method					515:520	an acid-catalyzed polyol in situ crosslinked phase separation method	453:520	an acid-catalyzed polyol in situ crosslinked phase separation method	453:520	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	28	theme	intestinal	179:188	arg1	efficiency					198:207	low intestinal release efficiency	175:207	low intestinal release efficiency	175:207	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	0	29	theme	Dual-stimuli	0:11	arg1	fibers					45:50	Dual-stimuli responsive skin-core structural fibers	0:50	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.	0:124	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.					
36880839	2	30	theme	reaction	553:560	arg1	mechanism					562:570	reaction mechanism	553:570	reaction mechanism	553:570	The reaction mechanism and apparent morphology of the fiber were studied.					
36880839	1	31	theme	separation	504:513	arg1	method					515:520	an acid-catalyzed polyol in situ crosslinked phase separation method	453:520	an acid-catalyzed polyol in situ crosslinked phase separation method	453:520	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	32	theme	release	190:196	arg1	efficiency					198:207	low intestinal release efficiency	175:207	low intestinal release efficiency	175:207	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	0	33	theme	structural	34:43	arg1	fibers					45:50	Dual-stimuli responsive skin-core structural fibers	0:50	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.	0:124	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.					
36880839	5	34	theme	release	921:927	arg1	rate					929:932	the release rate	917:932	the release rate of curcumin	917:944	2-FPBA controlled the release rate of curcumin by glucose stimulation, which increases with the increase of 2-FPBA content.					
36880839	5	35	theme	curcumin	937:944	arg1	rate					929:932	the release rate	917:932	the release rate of curcumin	917:944	2-FPBA controlled the release rate of curcumin by glucose stimulation, which increases with the increase of 2-FPBA content.					
36880839	1	36	theme	efficiency	198:207	arg1	problems					139:146	the problems	135:146	the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes	135:270	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	0	37	theme	skin-core	24:32	arg1	fibers					45:50	Dual-stimuli responsive skin-core structural fibers	0:50	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.	0:124	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.					
36880839	1	38	theme	novel	275:279	arg1	fiber					384:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber	273:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness	273:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	38	theme	novel	275:279	arg1	system					541:546	a drug delivery system	525:546	a drug delivery system	525:546	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	39	theme	drug	527:530	arg1	fiber					384:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber	273:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness	273:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	39	theme	drug	527:530	arg1	system					541:546	a drug delivery system	525:546	a drug delivery system	525:546	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	3	40	theme	simulated	670:678	arg1	liquids					680:686	simulated liquids	670:686	simulated liquids	670:686	The controlled release ability of the fiber in simulated liquids was evaluated.					
36880839	4	41	theme	release	762:768	arg1	amount					770:775	the release amount	758:775	the release amount in the simulated colonic fluid	758:806	AE targeted the release of curcumin by pH stimulation; the release amount in the simulated colonic fluid reached 100%, while the release amount in the simulated digestive fluid was less than 12%.					
36880839	5	42	theme	content	1014:1020	arg1	increase					995:1002	the increase	991:1002	the increase of 2-FPBA content	991:1020	2-FPBA controlled the release rate of curcumin by glucose stimulation, which increases with the increase of 2-FPBA content.					
36880839	7	43	theme	delivery	1204:1211	arg1	systems					1213:1219	curcumin delivery systems	1195:1219	curcumin delivery systems	1195:1219	These results suggest that skin-core structural fibers have great potential as curcumin delivery systems.					
36880839	7	43	theme	delivery	1204:1211	arg1	potential					1182:1190	great potential	1176:1190	great potential	1176:1190	These results suggest that skin-core structural fibers have great potential as curcumin delivery systems.					
36880839	1	44	theme	alginate	281:288	arg1	fiber					384:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber	273:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness	273:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	44	theme	alginate	281:288	arg1	system					541:546	a drug delivery system	525:546	a drug delivery system	525:546	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	45	theme	glucose	402:408	arg1	responsiveness					422:435	glucose stimulation responsiveness	402:435	glucose stimulation responsiveness	402:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	0	46	dep	in	60:61	arg1	situ					63:66	situ	63:66	situ	63:66	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.					
36880839	1	47	theme	curcumin	212:219	arg1	efficiency					198:207	low intestinal release efficiency	175:207	low intestinal release efficiency	175:207	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	47	theme	curcumin	212:219	arg1	bioavailability					155:169	low bioavailability	151:169	low bioavailability	151:169	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	7	48	theme	structural	1153:1162	arg1	fibers					1164:1169	skin-core structural fibers	1143:1169	skin-core structural fibers	1143:1169	These results suggest that skin-core structural fibers have great potential as curcumin delivery systems.					
36880839	1	49	theme	ester/Antarctic	290:304	arg1	fiber					384:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber	273:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness	273:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	49	theme	ester/Antarctic	290:304	arg1	system					541:546	a drug delivery system	525:546	a drug delivery system	525:546	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	50	theme	stimulation	410:420	arg1	responsiveness					422:435	glucose stimulation responsiveness	402:435	glucose stimulation responsiveness	402:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	3	51	theme	controlled	627:636	arg1	ability					646:652	The controlled release ability	623:652	The controlled release ability of the fiber in simulated liquids	623:686	The controlled release ability of the fiber in simulated liquids was evaluated.					
36880839	7	52	theme	curcumin	1195:1202	arg1	systems					1213:1219	curcumin delivery systems	1195:1219	curcumin delivery systems	1195:1219	These results suggest that skin-core structural fibers have great potential as curcumin delivery systems.					
36880839	7	52	theme	curcumin	1195:1202	arg1	potential					1182:1190	great potential	1176:1190	great potential	1176:1190	These results suggest that skin-core structural fibers have great potential as curcumin delivery systems.					
36880839	0	53	theme	in	60:61	arg1	ester					89:93	an in situ crosslinked alginate ester	57:93	an in situ crosslinked alginate ester for hydrophobic drug delivery	57:123	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.					
36880839	1	54	theme	krill	306:310	arg1	fiber					384:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber	273:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness	273:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	54	theme	krill	306:310	arg1	system					541:546	a drug delivery system	525:546	a drug delivery system	525:546	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	55	from	drug	238:241	arg1	treatment					250:258	the treatment	246:258	the treatment of diabetes	246:270	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	7	56	contain	have	1171:1174	arg2	potential					1182:1190	great potential	1176:1190	great potential	1176:1190	These results suggest that skin-core structural fibers have great potential as curcumin delivery systems.					
36880839	7	56	contain	have	1171:1174	arg2	systems					1213:1219	curcumin delivery systems	1195:1219	curcumin delivery systems	1195:1219	These results suggest that skin-core structural fibers have great potential as curcumin delivery systems.					
36880839	7	56	contain	have	1171:1174	arg1	fibers					1164:1169	skin-core structural fibers	1143:1169	skin-core structural fibers	1143:1169	These results suggest that skin-core structural fibers have great potential as curcumin delivery systems.					
36880839	4	57	theme	release	832:838	arg1	%					896:896	less than 12%	884:896	less than 12%	884:896	AE targeted the release of curcumin by pH stimulation; the release amount in the simulated colonic fluid reached 100%, while the release amount in the simulated digestive fluid was less than 12%.					
36880839	4	57	theme	release	832:838	arg1	amount					840:845	the release amount	828:845	the release amount in the simulated digestive fluid	828:878	AE targeted the release of curcumin by pH stimulation; the release amount in the simulated colonic fluid reached 100%, while the release amount in the simulated digestive fluid was less than 12%.					
36880839	1	58	theme	protein/2-formylphenylboronic	312:340	arg1	fiber					384:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber	273:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness	273:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	58	theme	protein/2-formylphenylboronic	312:340	arg1	system					541:546	a drug delivery system	525:546	a drug delivery system	525:546	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	2	59	theme	fiber	603:607	arg1	mechanism					562:570	reaction mechanism	553:570	reaction mechanism	553:570	The reaction mechanism and apparent morphology of the fiber were studied.					
36880839	2	59	theme	fiber	603:607	arg1	morphology					585:594	apparent morphology	576:594	apparent morphology	576:594	The reaction mechanism and apparent morphology of the fiber were studied.					
36880839	0	60	link	crosslinked	68:78	arg1	ester					89:93	an in situ crosslinked alginate ester	57:93	an in situ crosslinked alginate ester for hydrophobic drug delivery	57:123	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.					
36880839	3	61	theme	fiber	661:665	arg1	ability					646:652	The controlled release ability	623:652	The controlled release ability of the fiber in simulated liquids	623:686	The controlled release ability of the fiber in simulated liquids was evaluated.					
36880839	1	62	theme	acid	342:345	arg1	fiber					384:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber	273:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness	273:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	62	theme	acid	342:345	arg1	system					541:546	a drug delivery system	525:546	a drug delivery system	525:546	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	5	63	theme	2-FPBA	1007:1012	arg1	content					1014:1020	2-FPBA content	1007:1020	2-FPBA content	1007:1020	2-FPBA controlled the release rate of curcumin by glucose stimulation, which increases with the increase of 2-FPBA content.					
36880839	4	64	from	amount	770:775	arg1	fluid					802:806	the simulated colonic fluid	780:806	the simulated colonic fluid	780:806	AE targeted the release of curcumin by pH stimulation; the release amount in the simulated colonic fluid reached 100%, while the release amount in the simulated digestive fluid was less than 12%.					
36880839	1	65	theme	delivery	532:539	arg1	fiber					384:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber	273:388	a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness	273:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	65	theme	delivery	532:539	arg1	system					541:546	a drug delivery system	525:546	a drug delivery system	525:546	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	4	66	theme	pH	742:743	arg1	stimulation					745:755	pH stimulation	742:755	pH stimulation	742:755	AE targeted the release of curcumin by pH stimulation; the release amount in the simulated colonic fluid reached 100%, while the release amount in the simulated digestive fluid was less than 12%.					
36880839	1	67	theme	hydrophobic	226:236	arg1	drug					238:241	a hydrophobic drug	224:241	a hydrophobic drug in the treatment of diabetes	224:270	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	0	68	theme	crosslinked	68:78	arg1	ester					89:93	an in situ crosslinked alginate ester	57:93	an in situ crosslinked alginate ester for hydrophobic drug delivery	57:123	Dual-stimuli responsive skin-core structural fibers with an in situ crosslinked alginate ester for hydrophobic drug delivery.					
36880839	3	69	theme	release	638:644	arg1	ability					646:652	The controlled release ability	623:652	The controlled release ability of the fiber in simulated liquids	623:686	The controlled release ability of the fiber in simulated liquids was evaluated.					
36880839	6	70	theme	cytotoxicity	1037:1048	arg1	test					1050:1053	the cytotoxicity test	1033:1053	the cytotoxicity test	1033:1053	Moreover, the cytotoxicity test confirmed that the skin-core structural fiber was non-toxic.					
36880839	1	71	with	fiber	384:388	arg1	responsiveness					422:435	glucose stimulation responsiveness	402:435	glucose stimulation responsiveness	402:435	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	71	with	fiber	384:388	arg1	pH					395:396	pH	395:396	pH	395:396	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	1	72	link	crosslinked	486:496	arg1	method					515:520	an acid-catalyzed polyol in situ crosslinked phase separation method	453:520	an acid-catalyzed polyol in situ crosslinked phase separation method	453:520	To solve the problems of low bioavailability and low intestinal release efficiency of curcumin as a hydrophobic drug in the treatment of diabetes, a novel alginate ester/Antarctic krill protein/2-formylphenylboronic acid (AE/AKP/2-FPBA) skin-core structural fiber with pH and glucose stimulation responsiveness was prepared by an acid-catalyzed polyol in situ crosslinked phase separation method as a drug delivery system.					
36880839	7	73	theme	great	1176:1180	arg1	systems					1213:1219	curcumin delivery systems	1195:1219	curcumin delivery systems	1195:1219	These results suggest that skin-core structural fibers have great potential as curcumin delivery systems.					
36880839	7	73	theme	great	1176:1180	arg1	potential					1182:1190	great potential	1176:1190	great potential	1176:1190	These results suggest that skin-core structural fibers have great potential as curcumin delivery systems.					
36880839	4	74	theme	curcumin	730:737	arg1	release					719:725	the release	715:725	the release of curcumin by pH stimulation	715:755	AE targeted the release of curcumin by pH stimulation; the release amount in the simulated colonic fluid reached 100%, while the release amount in the simulated digestive fluid was less than 12%.					
37385315	4	0	theme	adsorption	715:724	arg1	process					726:732	the adsorption process	711:732	the adsorption process	711:732	The experimental results of Pb2+ adsorption showed that the adsorption process fitted well with the Langmuir monolayer adsorption and the pseudo-second-order models.					
37385315	4	1	theme	pseudo-second-order	793:811	arg1	models					813:818	the pseudo-second-order models	789:818	the pseudo-second-order models	789:818	The experimental results of Pb2+ adsorption showed that the adsorption process fitted well with the Langmuir monolayer adsorption and the pseudo-second-order models.					
37385315	0	2	theme	fractions	88:96	arg1	capacity					47:54	Pb2+ adsorption capacity	31:54	Pb2+ adsorption capacity	31:54	Physicochemical properties and Pb2+ adsorption capacity of freeze-dried hawthorn pectin fractions by gradient ethanol precipitation.					
37385315	0	2	theme	fractions	88:96	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and Pb2+ adsorption capacity of freeze-dried hawthorn pectin fractions by gradient ethanol precipitation.					
37385315	1	3	theme	FHP60	169:173	arg1	fractions					139:147	Three fractions	133:147	Three fractions of FHP20, FHP40 and FHP60	133:173	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
37385315	0	4	theme	ethanol	110:116	arg1	precipitation					118:130	gradient ethanol precipitation	101:130	gradient ethanol precipitation	101:130	Physicochemical properties and Pb2+ adsorption capacity of freeze-dried hawthorn pectin fractions by gradient ethanol precipitation.					
37385315	3	5	contain	had	521:523	arg1	FHP60					515:519	FHP60	515:519	FHP60	515:519	FHP60 had the lowest molecular weight (60.69 × 103 Da), and the composition and proportion of monosaccharides were significantly different.					
37385315	3	5	contain	had	521:523	arg2	60.69 × 103 Da					554:567	60.69 × 103 Da	554:567	60.69 × 103 Da	554:567	FHP60 had the lowest molecular weight (60.69 × 103 Da), and the composition and proportion of monosaccharides were significantly different.					
37385315	3	5	contain	had	521:523	arg2	weight					546:551	molecular weight	536:551	the lowest molecular weight (60.69 × 103 Da)	525:568	FHP60 had the lowest molecular weight (60.69 × 103 Da), and the composition and proportion of monosaccharides were significantly different.					
37385315	0	6	theme	gradient	101:108	arg1	precipitation					118:130	gradient ethanol precipitation	101:130	gradient ethanol precipitation	101:130	Physicochemical properties and Pb2+ adsorption capacity of freeze-dried hawthorn pectin fractions by gradient ethanol precipitation.					
37385315	5	7	theme	pectin	849:854	arg1	fractions					856:864	pectin fractions	849:864	pectin fractions with good homogeneity of molecular weight and chemical construction	849:932	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	1	8	from	performance	320:330	arg1	Pb2+					335:338	Pb2+	335:338	Pb2+	335:338	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
37385315	5	9	with	fractions	856:864	arg1	homogeneity					876:886	good homogeneity	871:886	good homogeneity of molecular weight and chemical construction	871:932	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	5	10	theme	weight	901:906	arg1	homogeneity					876:886	good homogeneity	871:886	good homogeneity of molecular weight and chemical construction	871:932	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	1	11	from	properties	294:303	arg1	Pb2+					335:338	Pb2+	335:338	Pb2+	335:338	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
37385315	3	12	theme	lowest	529:534	arg1	weight					546:551	molecular weight	536:551	the lowest molecular weight (60.69 × 103 Da)	525:568	FHP60 had the lowest molecular weight (60.69 × 103 Da), and the composition and proportion of monosaccharides were significantly different.					
37385315	3	12	theme	lowest	529:534	arg1	60.69 × 103 Da					554:567	60.69 × 103 Da	554:567	60.69 × 103 Da	554:567	FHP60 had the lowest molecular weight (60.69 × 103 Da), and the composition and proportion of monosaccharides were significantly different.					
37385315	5	13	theme	chemical	912:919	arg1	construction					921:932	chemical construction	912:932	chemical construction	912:932	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	2	14	theme	acid	405:408	arg1	content					381:387	the content	377:387	the content of galacturonic acid (GalA) and esterification of FHP fractions	377:451	It was found that the content of galacturonic acid (GalA) and esterification of FHP fractions gradually reduced with the increase of ethanol concentration.					
37385315	0	15	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and Pb2+ adsorption capacity of freeze-dried hawthorn pectin fractions by gradient ethanol precipitation.					
37385315	2	16	theme	galacturonic	392:403	arg1	GalA					411:414	GalA	411:414	GalA	411:414	It was found that the content of galacturonic acid (GalA) and esterification of FHP fractions gradually reduced with the increase of ethanol concentration.					
37385315	2	16	theme	galacturonic	392:403	arg1	acid					405:408	galacturonic acid	392:408	galacturonic acid (GalA)	392:415	It was found that the content of galacturonic acid (GalA) and esterification of FHP fractions gradually reduced with the increase of ethanol concentration.					
37385315	2	17	theme	fractions	443:451	arg1	GalA					411:414	GalA	411:414	GalA	411:414	It was found that the content of galacturonic acid (GalA) and esterification of FHP fractions gradually reduced with the increase of ethanol concentration.					
37385315	2	17	theme	fractions	443:451	arg1	esterification					421:434	esterification	421:434	esterification of FHP fractions	421:451	It was found that the content of galacturonic acid (GalA) and esterification of FHP fractions gradually reduced with the increase of ethanol concentration.					
37385315	2	17	theme	fractions	443:451	arg1	acid					405:408	galacturonic acid	392:408	galacturonic acid (GalA)	392:415	It was found that the content of galacturonic acid (GalA) and esterification of FHP fractions gradually reduced with the increase of ethanol concentration.					
37385315	2	18	theme	concentration	500:512	arg1	increase					480:487	the increase	476:487	the increase of ethanol concentration	476:512	It was found that the content of galacturonic acid (GalA) and esterification of FHP fractions gradually reduced with the increase of ethanol concentration.					
37385315	4	19	theme	Langmuir	755:762	arg1	adsorption					774:783	the Langmuir monolayer adsorption	751:783	the Langmuir monolayer adsorption	751:783	The experimental results of Pb2+ adsorption showed that the adsorption process fitted well with the Langmuir monolayer adsorption and the pseudo-second-order models.					
37385315	4	20	theme	adsorption	688:697	arg1	results					672:678	The experimental results	655:678	The experimental results of Pb2+ adsorption	655:697	The experimental results of Pb2+ adsorption showed that the adsorption process fitted well with the Langmuir monolayer adsorption and the pseudo-second-order models.					
37385315	2	21	theme	FHP	439:441	arg1	fractions					443:451	FHP fractions	439:451	FHP fractions	439:451	It was found that the content of galacturonic acid (GalA) and esterification of FHP fractions gradually reduced with the increase of ethanol concentration.					
37385315	5	22	gly	homogeneity	876:886	arg1	weight					901:906	molecular weight	891:906	molecular weight	891:906	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	5	22	gly	homogeneity	876:886	arg1	construction					921:932	chemical construction	912:932	chemical construction	912:932	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	1	23	theme	freeze-dried	194:205	arg1	pectin					216:221	freeze-dried hawthorn pectin	194:221	freeze-dried hawthorn pectin	194:221	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
37385315	0	24	theme	Pb2+	31:34	arg1	capacity					47:54	Pb2+ adsorption capacity	31:54	Pb2+ adsorption capacity	31:54	Physicochemical properties and Pb2+ adsorption capacity of freeze-dried hawthorn pectin fractions by gradient ethanol precipitation.					
37385315	1	25	theme	physicochemical	278:292	arg1	properties					294:303	their physicochemical properties	272:303	their physicochemical properties	272:303	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
37385315	1	26	theme	hawthorn	207:214	arg1	pectin					216:221	freeze-dried hawthorn pectin	194:221	freeze-dried hawthorn pectin	194:221	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
37385315	2	27	theme	esterification	421:434	arg1	content					381:387	the content	377:387	the content of galacturonic acid (GalA) and esterification of FHP fractions	377:451	It was found that the content of galacturonic acid (GalA) and esterification of FHP fractions gradually reduced with the increase of ethanol concentration.					
37385315	5	28	theme	hawthorn	989:996	arg1	pectin					998:1003	hawthorn pectin	989:1003	hawthorn pectin	989:1003	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	5	28	theme	hawthorn	989:996	arg1	potential					1029:1037	a potential	1027:1037	a potential adsorbent for Pb2+ removal	1027:1064	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	4	29	theme	monolayer	764:772	arg1	adsorption					774:783	the Langmuir monolayer adsorption	751:783	the Langmuir monolayer adsorption	751:783	The experimental results of Pb2+ adsorption showed that the adsorption process fitted well with the Langmuir monolayer adsorption and the pseudo-second-order models.					
37385315	4	30	theme	Pb2+	683:686	arg1	adsorption					688:697	Pb2+ adsorption	683:697	Pb2+ adsorption	683:697	The experimental results of Pb2+ adsorption showed that the adsorption process fitted well with the Langmuir monolayer adsorption and the pseudo-second-order models.					
37385315	4	31	theme	experimental	659:670	arg1	results					672:678	The experimental results	655:678	The experimental results of Pb2+ adsorption	655:697	The experimental results of Pb2+ adsorption showed that the adsorption process fitted well with the Langmuir monolayer adsorption and the pseudo-second-order models.					
37385315	0	32	theme	adsorption	36:45	arg1	capacity					47:54	Pb2+ adsorption capacity	31:54	Pb2+ adsorption capacity	31:54	Physicochemical properties and Pb2+ adsorption capacity of freeze-dried hawthorn pectin fractions by gradient ethanol precipitation.					
37385315	1	33	theme	adsorption	309:318	arg1	performance					320:330	adsorption performance	309:330	adsorption performance	309:330	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
37385315	1	34	theme	gradient	226:233	arg1	precipitation					243:255	gradient ethanol precipitation	226:255	gradient ethanol precipitation (20-60 %)	226:265	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
37385315	1	34	theme	gradient	226:233	arg1	%					264:264	20-60 %	258:264	20-60 %	258:264	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
37385315	0	35	theme	freeze-dried	59:70	arg1	fractions					88:96	freeze-dried hawthorn pectin fractions	59:96	freeze-dried hawthorn pectin fractions	59:96	Physicochemical properties and Pb2+ adsorption capacity of freeze-dried hawthorn pectin fractions by gradient ethanol precipitation.					
37385315	0	36	theme	hawthorn	72:79	arg1	fractions					88:96	freeze-dried hawthorn pectin fractions	59:96	freeze-dried hawthorn pectin fractions	59:96	Physicochemical properties and Pb2+ adsorption capacity of freeze-dried hawthorn pectin fractions by gradient ethanol precipitation.					
37385315	5	37	theme	gradient	953:960	arg1	precipitation					970:982	gradient ethanol precipitation	953:982	gradient ethanol precipitation	953:982	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	5	38	theme	ethanol	962:968	arg1	precipitation					970:982	gradient ethanol precipitation	953:982	gradient ethanol precipitation	953:982	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	5	39	theme	Pb2+	1053:1056	arg1	removal					1058:1064	Pb2+ removal	1053:1064	Pb2+ removal	1053:1064	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	5	40	theme	construction	921:932	arg1	homogeneity					876:886	good homogeneity	871:886	good homogeneity of molecular weight and chemical construction	871:932	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	1	41	theme	ethanol	235:241	arg1	precipitation					243:255	gradient ethanol precipitation	226:255	gradient ethanol precipitation (20-60 %)	226:265	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
37385315	1	41	theme	ethanol	235:241	arg1	%					264:264	20-60 %	258:264	20-60 %	258:264	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
37385315	3	42	theme	molecular	536:544	arg1	weight					546:551	molecular weight	536:551	the lowest molecular weight (60.69 × 103 Da)	525:568	FHP60 had the lowest molecular weight (60.69 × 103 Da), and the composition and proportion of monosaccharides were significantly different.					
37385315	3	42	theme	molecular	536:544	arg1	60.69 × 103 Da					554:567	60.69 × 103 Da	554:567	60.69 × 103 Da	554:567	FHP60 had the lowest molecular weight (60.69 × 103 Da), and the composition and proportion of monosaccharides were significantly different.					
37385315	5	43	theme	adsorbent	1039:1047	arg1	pectin					998:1003	hawthorn pectin	989:1003	hawthorn pectin	989:1003	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	5	43	theme	adsorbent	1039:1047	arg1	potential					1029:1037	a potential	1027:1037	a potential adsorbent for Pb2+ removal	1027:1064	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	1	44	theme	FHP20	152:156	arg1	fractions					139:147	Three fractions	133:147	Three fractions of FHP20, FHP40 and FHP60	133:173	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
37385315	0	45	theme	pectin	81:86	arg1	fractions					88:96	freeze-dried hawthorn pectin fractions	59:96	freeze-dried hawthorn pectin fractions	59:96	Physicochemical properties and Pb2+ adsorption capacity of freeze-dried hawthorn pectin fractions by gradient ethanol precipitation.					
37385315	5	46	theme	molecular	891:899	arg1	weight					901:906	molecular weight	891:906	molecular weight	891:906	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	5	47	theme	good	871:874	arg1	homogeneity					876:886	good homogeneity	871:886	good homogeneity of molecular weight and chemical construction	871:932	Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.					
37385315	3	48	dep	composition	579:589	arg1	the					575:577	the	575:577	the	575:577	FHP60 had the lowest molecular weight (60.69 × 103 Da), and the composition and proportion of monosaccharides were significantly different.					
37385315	2	49	theme	ethanol	492:498	arg1	concentration					500:512	ethanol concentration	492:512	ethanol concentration	492:512	It was found that the content of galacturonic acid (GalA) and esterification of FHP fractions gradually reduced with the increase of ethanol concentration.					
37385315	3	50	theme	monosaccharides	609:623	arg1	proportion					595:604	proportion	595:604	proportion	595:604	FHP60 had the lowest molecular weight (60.69 × 103 Da), and the composition and proportion of monosaccharides were significantly different.					
37385315	3	50	theme	monosaccharides	609:623	arg1	composition					579:589	composition	579:589	composition	579:589	FHP60 had the lowest molecular weight (60.69 × 103 Da), and the composition and proportion of monosaccharides were significantly different.					
37385315	1	51	theme	FHP40	159:163	arg1	fractions					139:147	Three fractions	133:147	Three fractions of FHP20, FHP40 and FHP60	133:173	Three fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated.					
35725149	8	0	theme	chemical	1122:1129	arg1	treatment					1073:1081	DES treatment	1069:1081	DES treatment	1069:1081	To conclude, DES treatment can be considered as a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation.					
35725149	8	0	theme	chemical	1122:1129	arg1	"					1130:1130	a soft and green "chemical"	1104:1130	a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation	1104:1212	To conclude, DES treatment can be considered as a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation.					
35725149	8	1	theme	green	1115:1119	arg1	treatment					1073:1081	DES treatment	1069:1081	DES treatment	1069:1081	To conclude, DES treatment can be considered as a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation.					
35725149	8	1	theme	green	1115:1119	arg1	"					1130:1130	a soft and green "chemical"	1104:1130	a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation	1104:1212	To conclude, DES treatment can be considered as a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation.					
35725149	3	2	dep	Three	335:339	arg1	alkaline					380:387	alkaline	380:387	alkaline	380:387	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	2	dep	Three	335:339	arg1	neutral					368:374	neutral	368:374	neutral	368:374	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	2	dep	Three	335:339	arg1	acid					362:365	acid	362:365	acid	362:365	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	2	dep	Three	335:339	arg1	DES					341:343	DES	341:343	Three DES of different pH (acid, neutral and alkaline)	335:388	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	2	3	theme	microfibrillated	307:322	arg1	cellulose					324:332	microfibrillated cellulose	307:332	microfibrillated cellulose	307:332	A focus was made on the induced fibrillation phenomena which could facilitate the further production of microfibrillated cellulose.					
35725149	4	4	used	used	533:536	arg2	pulps					522:526	Eucalyptus and cotton pulps	500:526	Eucalyptus and cotton pulps	500:526	Eucalyptus and cotton pulps were used to investigate the effects of DES on both hemicelluloses and cellulose.					
35725149	7	5	theme	cellulose	947:955	arg1	microfibrillation					957:973	cellulose microfibrillation	947:973	cellulose microfibrillation	947:973	Compared to enzymatic hydrolysis (used as a conventional pre-treatment for cellulose microfibrillation), DES have significantly enhanced the mechanical properties of resulting papers.					
35725149	4	6	from	effects	557:563	arg1	cellulose					599:607	cellulose	599:607	cellulose	599:607	Eucalyptus and cotton pulps were used to investigate the effects of DES on both hemicelluloses and cellulose.					
35725149	4	6	from	effects	557:563	arg1	hemicelluloses					580:593	hemicelluloses	580:593	hemicelluloses	580:593	Eucalyptus and cotton pulps were used to investigate the effects of DES on both hemicelluloses and cellulose.					
35725149	0	7	from	Effects	0:6	arg1	properties					65:74	paper properties	59:74	paper properties	59:74	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.					
35725149	0	7	from	Effects	0:6	arg1	fibres					48:53	cellulosic fibres	37:53	cellulosic fibres	37:53	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.					
35725149	6	8	theme	modification	781:792	arg1	extent					771:776	a great extent	763:776	a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation	763:869	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	2	9	theme	induced	227:233	arg1	phenomena					248:256	the induced fibrillation phenomena	223:256	the induced fibrillation phenomena which could facilitate the further production of microfibrillated cellulose	223:332	A focus was made on the induced fibrillation phenomena which could facilitate the further production of microfibrillated cellulose.					
35725149	0	10	theme	Green	77:81	arg1	"					92:92	Green "chemical"	77:92	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.	0:102	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.					
35725149	7	11	dep	hydrolysis	894:903	arg1	used					906:909	used	906:909	used as a conventional pre-treatment for cellulose microfibrillation	906:973	Compared to enzymatic hydrolysis (used as a conventional pre-treatment for cellulose microfibrillation), DES have significantly enhanced the mechanical properties of resulting papers.					
35725149	3	12	theme	choline	452:458	arg1	chloride-monoethanolamine					460:484	choline chloride-monoethanolamine	452:484	choline chloride-monoethanolamine	452:484	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	12	theme	choline	452:458	arg1	Three					335:339	Three	335:339	Three	335:339	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	12	theme	choline	452:458	arg1	pH					358:359	different pH	348:359	different pH	348:359	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	0	13	theme	chemical	84:91	arg1	"					92:92	Green "chemical"	77:92	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.	0:102	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.					
35725149	6	14	theme	polymerisation	856:869	arg1	crystallinity					828:840	crystallinity	828:840	crystallinity	828:840	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	6	14	theme	polymerisation	856:869	arg1	composition					815:825	chemical composition	806:825	chemical composition	806:825	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	6	14	theme	polymerisation	856:869	arg1	degree					846:851	degree	846:851	degree	846:851	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	6	15	theme	great	765:769	arg1	extent					771:776	a great extent	763:776	a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation	763:869	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	6	16	from	extent	771:776	arg1	terms					797:801	terms	797:801	terms of chemical composition, crystallinity and degree of polymerisation	797:869	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	7	17	theme	conventional	916:927	arg1	pre-treatment					929:941	a conventional pre-treatment	914:941	a conventional pre-treatment	914:941	Compared to enzymatic hydrolysis (used as a conventional pre-treatment for cellulose microfibrillation), DES have significantly enhanced the mechanical properties of resulting papers.					
35725149	1	18	theme	Deep	143:146	arg1	DES					167:169	DES	167:169	DES	167:169	This work aims to study the effects of Deep Eutectic Solvents (DES) on cellulosic fibre structure.					
35725149	1	18	theme	Deep	143:146	arg1	Solvents					157:164	Deep Eutectic Solvents	143:164	Deep Eutectic Solvents (DES)	143:170	This work aims to study the effects of Deep Eutectic Solvents (DES) on cellulosic fibre structure.					
35725149	3	19	theme	different	348:356	arg1	pH					358:359	different pH	348:359	different pH	348:359	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	1	20	theme	Eutectic	148:155	arg1	DES					167:169	DES	167:169	DES	167:169	This work aims to study the effects of Deep Eutectic Solvents (DES) on cellulosic fibre structure.					
35725149	1	20	theme	Eutectic	148:155	arg1	Solvents					157:164	Deep Eutectic Solvents	143:164	Deep Eutectic Solvents (DES)	143:170	This work aims to study the effects of Deep Eutectic Solvents (DES) on cellulosic fibre structure.					
35725149	0	21	theme	Eutectic	16:23	arg1	Solvents					25:32	Deep Eutectic Solvents	11:32	Deep Eutectic Solvents	11:32	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.					
35725149	4	22	theme	cotton	515:520	arg1	pulps					522:526	Eucalyptus and cotton pulps	500:526	Eucalyptus and cotton pulps	500:526	Eucalyptus and cotton pulps were used to investigate the effects of DES on both hemicelluloses and cellulose.					
35725149	3	23	theme	betaine	398:404	arg1	Three					335:339	Three	335:339	Three	335:339	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	23	theme	betaine	398:404	arg1	pH					358:359	different pH	348:359	different pH	348:359	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	23	theme	betaine	398:404	arg1	hydrochloride-urea					406:423	betaine hydrochloride-urea	398:423	betaine hydrochloride-urea	398:423	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	0	24	theme	Deep	11:14	arg1	Solvents					25:32	Deep Eutectic Solvents	11:32	Deep Eutectic Solvents	11:32	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.					
35725149	8	25	theme	DES	1069:1071	arg1	treatment					1073:1081	DES treatment	1069:1081	DES treatment	1069:1081	To conclude, DES treatment can be considered as a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation.					
35725149	8	25	theme	DES	1069:1071	arg1	"					1130:1130	a soft and green "chemical"	1104:1130	a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation	1104:1212	To conclude, DES treatment can be considered as a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation.					
35725149	6	26	theme	degree	846:851	arg1	terms					797:801	terms	797:801	terms of chemical composition, crystallinity and degree of polymerisation	797:869	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	2	27	theme	fibrillation	235:246	arg1	phenomena					248:256	the induced fibrillation phenomena	223:256	the induced fibrillation phenomena which could facilitate the further production of microfibrillated cellulose	223:332	A focus was made on the induced fibrillation phenomena which could facilitate the further production of microfibrillated cellulose.					
35725149	6	28	from	modification	781:792	arg1	terms					797:801	terms	797:801	terms of chemical composition, crystallinity and degree of polymerisation	797:869	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	0	29	theme	Solvents	25:32	arg1	Effects					0:6	Effects	0:6	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.	0:102	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.					
35725149	6	30	from	terms	797:801	arg1	extent					771:776	a great extent	763:776	a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation	763:869	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	6	31	theme	crystallinity	828:840	arg1	terms					797:801	terms	797:801	terms of chemical composition, crystallinity and degree of polymerisation	797:869	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	6	32	theme	DES	741:743	arg1	treatment					745:753	DES treatment	741:753	DES treatment	741:753	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	3	33	theme	pH	358:359	arg1	chloride-urea					434:446	choline chloride-urea	426:446	choline chloride-urea	426:446	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	33	theme	pH	358:359	arg1	chloride-monoethanolamine					460:484	choline chloride-monoethanolamine	452:484	choline chloride-monoethanolamine	452:484	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	33	theme	pH	358:359	arg1	Three					335:339	Three	335:339	Three	335:339	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	33	theme	pH	358:359	arg1	pH					358:359	different pH	348:359	different pH	348:359	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	33	theme	pH	358:359	arg1	hydrochloride-urea					406:423	betaine hydrochloride-urea	398:423	betaine hydrochloride-urea	398:423	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	7	34	theme	resulting	1038:1046	arg1	papers					1048:1053	resulting papers	1038:1053	resulting papers	1038:1053	Compared to enzymatic hydrolysis (used as a conventional pre-treatment for cellulose microfibrillation), DES have significantly enhanced the mechanical properties of resulting papers.					
35725149	7	35	theme	mechanical	1013:1022	arg1	properties					1024:1033	the mechanical properties	1009:1033	the mechanical properties of resulting papers	1009:1053	Compared to enzymatic hydrolysis (used as a conventional pre-treatment for cellulose microfibrillation), DES have significantly enhanced the mechanical properties of resulting papers.					
35725149	0	36	theme	cellulosic	37:46	arg1	fibres					48:53	cellulosic fibres	37:53	cellulosic fibres	37:53	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.					
35725149	7	37	theme	papers	1048:1053	arg1	properties					1024:1033	the mechanical properties	1009:1033	the mechanical properties of resulting papers	1009:1053	Compared to enzymatic hydrolysis (used as a conventional pre-treatment for cellulose microfibrillation), DES have significantly enhanced the mechanical properties of resulting papers.					
35725149	6	38	theme	composition	815:825	arg1	terms					797:801	terms	797:801	terms of chemical composition, crystallinity and degree of polymerisation	797:869	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	2	39	theme	further	285:291	arg1	production					293:302	the further production	281:302	the further production of microfibrillated cellulose	281:332	A focus was made on the induced fibrillation phenomena which could facilitate the further production of microfibrillated cellulose.					
35725149	4	40	theme	Eucalyptus	500:509	arg1	pulps					522:526	Eucalyptus and cotton pulps	500:526	Eucalyptus and cotton pulps	500:526	Eucalyptus and cotton pulps were used to investigate the effects of DES on both hemicelluloses and cellulose.					
35725149	5	41	theme	acidic	657:662	arg1	treatment					668:676	acidic DES treatment	657:676	acidic DES treatment	657:676	Interestingly, cellulose was esterified during acidic DES treatment.					
35725149	6	42	theme	chemical	806:813	arg1	composition					815:825	chemical composition	806:825	chemical composition	806:825	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	8	43	theme	soft	1106:1109	arg1	treatment					1073:1081	DES treatment	1069:1081	DES treatment	1069:1081	To conclude, DES treatment can be considered as a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation.					
35725149	8	43	theme	soft	1106:1109	arg1	"					1130:1130	a soft and green "chemical"	1104:1130	a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation	1104:1212	To conclude, DES treatment can be considered as a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation.					
35725149	6	44	theme	internal	692:699	arg1	fibrillation					714:725	an internal and external fibrillation	689:725	an internal and external fibrillation	689:725	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	4	45	theme	DES	568:570	arg1	effects					557:563	the effects	553:563	the effects of DES on both hemicelluloses and cellulose	553:607	Eucalyptus and cotton pulps were used to investigate the effects of DES on both hemicelluloses and cellulose.					
35725149	5	46	theme	DES	664:666	arg1	treatment					668:676	acidic DES treatment	657:676	acidic DES treatment	657:676	Interestingly, cellulose was esterified during acidic DES treatment.					
35725149	8	47	theme	cellulose	1186:1194	arg1	microfibrillation					1196:1212	cellulose microfibrillation	1186:1212	cellulose microfibrillation	1186:1212	To conclude, DES treatment can be considered as a soft and green "chemical" refining that could be applied as a pre-treatment for cellulose microfibrillation.					
35725149	6	48	theme	external	705:712	arg1	fibrillation					714:725	an internal and external fibrillation	689:725	an internal and external fibrillation	689:725	Moreover, an internal and external fibrillation occurred with DES treatment without a great extent of modification in terms of chemical composition, crystallinity and degree of polymerisation.					
35725149	3	49	theme	choline	426:432	arg1	chloride-urea					434:446	choline chloride-urea	426:446	choline chloride-urea	426:446	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	49	theme	choline	426:432	arg1	Three					335:339	Three	335:339	Three	335:339	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	3	49	theme	choline	426:432	arg1	pH					358:359	different pH	348:359	different pH	348:359	Three DES of different pH (acid, neutral and alkaline), namely betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine, were tested.					
35725149	1	50	theme	cellulosic	175:184	arg1	structure					192:200	cellulosic fibre structure	175:200	cellulosic fibre structure	175:200	This work aims to study the effects of Deep Eutectic Solvents (DES) on cellulosic fibre structure.					
35725149	0	51	theme	paper	59:63	arg1	properties					65:74	paper properties	59:74	paper properties	59:74	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.					
35725149	1	52	theme	Solvents	157:164	arg1	effects					132:138	the effects	128:138	the effects of Deep Eutectic Solvents (DES) on cellulosic fibre structure	128:200	This work aims to study the effects of Deep Eutectic Solvents (DES) on cellulosic fibre structure.					
35725149	7	53	theme	enzymatic	884:892	arg1	hydrolysis					894:903	enzymatic hydrolysis	884:903	enzymatic hydrolysis (used as a conventional pre-treatment for cellulose microfibrillation)	884:974	Compared to enzymatic hydrolysis (used as a conventional pre-treatment for cellulose microfibrillation), DES have significantly enhanced the mechanical properties of resulting papers.					
35725149	1	54	theme	fibre	186:190	arg1	structure					192:200	cellulosic fibre structure	175:200	cellulosic fibre structure	175:200	This work aims to study the effects of Deep Eutectic Solvents (DES) on cellulosic fibre structure.					
35725149	0	55	dep	Effects	0:6	arg1	"					92:92	Green "chemical"	77:92	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.	0:102	Effects of Deep Eutectic Solvents on cellulosic fibres and paper properties: Green "chemical" refining.					
35725149	1	56	from	effects	132:138	arg1	structure					192:200	cellulosic fibre structure	175:200	cellulosic fibre structure	175:200	This work aims to study the effects of Deep Eutectic Solvents (DES) on cellulosic fibre structure.					
35725149	2	57	theme	cellulose	324:332	arg1	production					293:302	the further production	281:302	the further production of microfibrillated cellulose	281:332	A focus was made on the induced fibrillation phenomena which could facilitate the further production of microfibrillated cellulose.					
36996962	0	0	theme	antibacterial	94:106	arg1	film					108:111	cellulose active antibacterial film	77:111	cellulose active antibacterial film	77:111	Construction and application of nano ZnO/eugenol@yam starch/microcrystalline cellulose active antibacterial film.					
36996962	7	1	theme	synergistic	1008:1018	arg1	effect					1034:1039	a synergistic antibacterial effect	1006:1039	a synergistic antibacterial effect	1006:1039	After loading Eu, the antimicrobial activity of ZnO:Eu@SC was significantly enhanced, resulting in a synergistic antibacterial effect.					
36996962	5	2	from	release	757:763	arg1	simulants					792:800	food simulants	787:800	food simulants	787:800	ZnO:Eu@SC exhibited good controlled release of nano-ZnO and Eu in food simulants.					
36996962	1	3	theme	new	152:154	arg1	films					169:173	new biocomposite films	152:173	new biocomposite films that can better protect and prolong the shelf life of food	152:232	The goal of this study was to develop new biocomposite films that can better protect and prolong the shelf life of food.					
36996962	0	4	theme	active	87:92	arg1	film					108:111	cellulose active antibacterial film	77:111	cellulose active antibacterial film	77:111	Construction and application of nano ZnO/eugenol@yam starch/microcrystalline cellulose active antibacterial film.					
36996962	9	5	theme	Eu	1121:1122	arg1	film					1127:1130	the ZnO:Eu@SC film	1113:1130	the ZnO:Eu@SC film	1113:1130	In humus, the ZnO:Eu@SC film was effectively degraded into fragments.					
36996962	7	6	theme	antibacterial	1020:1032	arg1	effect					1034:1039	a synergistic antibacterial effect	1006:1039	a synergistic antibacterial effect	1006:1039	After loading Eu, the antimicrobial activity of ZnO:Eu@SC was significantly enhanced, resulting in a synergistic antibacterial effect.					
36996962	10	7	theme	SC	1195:1196	arg1	film					1198:1201	the ZnO:Eu@SC film	1184:1201	the ZnO:Eu@SC film	1184:1201	Therefore, the ZnO:Eu@SC film has excellent potential in food active packaging.					
36996962	1	8	theme	biocomposite	156:167	arg1	films					169:173	new biocomposite films	152:173	new biocomposite films that can better protect and prolong the shelf life of food	152:232	The goal of this study was to develop new biocomposite films that can better protect and prolong the shelf life of food.					
36996962	10	9	contain	has	1203:1205	arg2	potential					1217:1225	excellent potential	1207:1225	excellent potential	1207:1225	Therefore, the ZnO:Eu@SC film has excellent potential in food active packaging.					
36996962	10	9	contain	has	1203:1205	arg1	film					1198:1201	the ZnO:Eu@SC film	1184:1201	the ZnO:Eu@SC film	1184:1201	Therefore, the ZnO:Eu@SC film has excellent potential in food active packaging.					
36996962	8	10	theme	@	1047:1047	arg1	film					1051:1054	Eu@SC film	1045:1054	Eu@SC film	1045:1054	Z4:Eu@SC film extended the pork shelf life by 100 % (25 °C).					
36996962	9	11	theme	@	1123:1123	arg1	film					1127:1130	the ZnO:Eu@SC film	1113:1130	the ZnO:Eu@SC film	1113:1130	In humus, the ZnO:Eu@SC film was effectively degraded into fragments.					
36996962	10	12	theme	food	1230:1233	arg1	packaging					1242:1250	food active packaging	1230:1250	food active packaging	1230:1250	Therefore, the ZnO:Eu@SC film has excellent potential in food active packaging.					
36996962	10	13	theme	@	1194:1194	arg1	film					1198:1201	the ZnO:Eu@SC film	1184:1201	the ZnO:Eu@SC film	1184:1201	Therefore, the ZnO:Eu@SC film has excellent potential in food active packaging.					
36996962	2	14	theme	@	255:255	arg1	cellulose					284:292	a ZnO: eugenol@yam starch/microcrystalline cellulose	241:292	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	2	14	theme	@	255:255	arg1	SC					302:303	ZnO:Eu@SC	295:303	ZnO:Eu@SC	295:303	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	3	15	theme	films	520:524	arg1	properties					496:505	the physicochemical and functional properties	461:505	the physicochemical and functional properties of composite films	461:524	Because of the advantages of metal oxides and plant essential oils, codoping with these can effectively improve the physicochemical and functional properties of composite films.					
36996962	4	16	theme	appropriate	546:556	arg1	nano-ZnO					568:575	nano-ZnO	568:575	nano-ZnO	568:575	The addition of an appropriate amount of nano-ZnO improved the compactness and thermostability, reduced the moisture sensitivity, and enhanced the mechanical and barrier properties of the film.					
36996962	4	16	theme	appropriate	546:556	arg1	amount					558:563	an appropriate amount	543:563	an appropriate amount of nano-ZnO	543:575	The addition of an appropriate amount of nano-ZnO improved the compactness and thermostability, reduced the moisture sensitivity, and enhanced the mechanical and barrier properties of the film.					
36996962	8	17	theme	Eu	1045:1046	arg1	film					1051:1054	Eu@SC film	1045:1054	Eu@SC film	1045:1054	Z4:Eu@SC film extended the pork shelf life by 100 % (25 °C).					
36996962	9	18	theme	SC	1124:1125	arg1	film					1127:1130	the ZnO:Eu@SC film	1113:1130	the ZnO:Eu@SC film	1113:1130	In humus, the ZnO:Eu@SC film was effectively degraded into fragments.					
36996962	10	19	theme	Eu	1192:1193	arg1	film					1198:1201	the ZnO:Eu@SC film	1184:1201	the ZnO:Eu@SC film	1184:1201	Therefore, the ZnO:Eu@SC film has excellent potential in food active packaging.					
36996962	2	20	theme	eugenol	248:254	arg1	cellulose					284:292	a ZnO: eugenol@yam starch/microcrystalline cellulose	241:292	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	2	20	theme	eugenol	248:254	arg1	SC					302:303	ZnO:Eu@SC	295:303	ZnO:Eu@SC	295:303	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	6	21	dep	mechanisms	849:858	arg1	swelling					885:892	swelling	885:892	swelling (secondary)	885:904	Nano-ZnO and Eu release was controlled by two mechanisms: diffusion (primary) and swelling (secondary).					
36996962	6	21	dep	mechanisms	849:858	arg1	diffusion					861:869	diffusion	861:869	diffusion (primary)	861:879	Nano-ZnO and Eu release was controlled by two mechanisms: diffusion (primary) and swelling (secondary).					
36996962	6	21	dep	mechanisms	849:858	arg1	mechanisms					849:858	two mechanisms	845:858	two mechanisms: diffusion (primary) and swelling (secondary)	845:904	Nano-ZnO and Eu release was controlled by two mechanisms: diffusion (primary) and swelling (secondary).					
36996962	5	22	theme	Eu	725:726	arg1	SC					728:729	Eu@SC	725:729	Eu@SC	725:729	ZnO:Eu@SC exhibited good controlled release of nano-ZnO and Eu in food simulants.					
36996962	4	23	theme	mechanical	674:683	arg1	properties					697:706	the mechanical and barrier properties	670:706	properties	697:706	The addition of an appropriate amount of nano-ZnO improved the compactness and thermostability, reduced the moisture sensitivity, and enhanced the mechanical and barrier properties of the film.					
36996962	3	24	with	codoping	417:424	arg1	these					431:435	these	431:435	these	431:435	Because of the advantages of metal oxides and plant essential oils, codoping with these can effectively improve the physicochemical and functional properties of composite films.					
36996962	10	25	theme	active	1235:1240	arg1	packaging					1242:1250	food active packaging	1230:1250	food active packaging	1230:1250	Therefore, the ZnO:Eu@SC film has excellent potential in food active packaging.					
36996962	8	26	theme	pork	1069:1072	arg1	life					1080:1083	the pork shelf life	1065:1083	the pork shelf life	1065:1083	Z4:Eu@SC film extended the pork shelf life by 100 % (25 °C).					
36996962	3	27	theme	oxides	384:389	arg1	advantages					364:373	the advantages	360:373	the advantages of metal oxides and plant essential oils	360:414	Because of the advantages of metal oxides and plant essential oils, codoping with these can effectively improve the physicochemical and functional properties of composite films.					
36996962	5	28	theme	good	741:744	arg1	release					757:763	good controlled release	741:763	good controlled release of nano-ZnO and Eu in food simulants	741:800	ZnO:Eu@SC exhibited good controlled release of nano-ZnO and Eu in food simulants.					
36996962	5	29	theme	@	727:727	arg1	SC					728:729	Eu@SC	725:729	Eu@SC	725:729	ZnO:Eu@SC exhibited good controlled release of nano-ZnO and Eu in food simulants.					
36996962	2	30	theme	ZnO	243:245	arg1	cellulose					284:292	a ZnO: eugenol@yam starch/microcrystalline cellulose	241:292	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	2	30	theme	ZnO	243:245	arg1	SC					302:303	ZnO:Eu@SC	295:303	ZnO:Eu@SC	295:303	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	7	31	dep	ZnO	955:957	arg1	SC					962:963	Eu@SC	959:963	ZnO:Eu@SC	955:963	After loading Eu, the antimicrobial activity of ZnO:Eu@SC was significantly enhanced, resulting in a synergistic antibacterial effect.					
36996962	5	32	theme	controlled	746:755	arg1	release					757:763	good controlled release	741:763	good controlled release of nano-ZnO and Eu in food simulants	741:800	ZnO:Eu@SC exhibited good controlled release of nano-ZnO and Eu in food simulants.					
36996962	3	33	theme	physicochemical	465:479	arg1	properties					496:505	the physicochemical and functional properties	461:505	the physicochemical and functional properties of composite films	461:524	Because of the advantages of metal oxides and plant essential oils, codoping with these can effectively improve the physicochemical and functional properties of composite films.					
36996962	10	34	theme	excellent	1207:1215	arg1	potential					1217:1225	excellent potential	1207:1225	excellent potential	1207:1225	Therefore, the ZnO:Eu@SC film has excellent potential in food active packaging.					
36996962	4	35	theme	film	715:718	arg1	properties					697:706	the mechanical and barrier properties	670:706	properties	697:706	The addition of an appropriate amount of nano-ZnO improved the compactness and thermostability, reduced the moisture sensitivity, and enhanced the mechanical and barrier properties of the film.					
36996962	4	36	theme	moisture	635:642	arg1	sensitivity					644:654	the moisture sensitivity	631:654	the moisture sensitivity	631:654	The addition of an appropriate amount of nano-ZnO improved the compactness and thermostability, reduced the moisture sensitivity, and enhanced the mechanical and barrier properties of the film.					
36996962	2	37	theme	cellulose	284:292	arg1	film					327:330	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	3	38	theme	metal	378:382	arg1	oxides					384:389	metal oxides	378:389	metal oxides	378:389	Because of the advantages of metal oxides and plant essential oils, codoping with these can effectively improve the physicochemical and functional properties of composite films.					
36996962	8	39	theme	shelf	1074:1078	arg1	life					1080:1083	the pork shelf life	1065:1083	the pork shelf life	1065:1083	Z4:Eu@SC film extended the pork shelf life by 100 % (25 °C).					
36996962	8	40	theme	SC	1048:1049	arg1	film					1051:1054	Eu@SC film	1045:1054	Eu@SC film	1045:1054	Z4:Eu@SC film extended the pork shelf life by 100 % (25 °C).					
36996962	2	41	theme	starch/microcrystalline	260:282	arg1	cellulose					284:292	a ZnO: eugenol@yam starch/microcrystalline cellulose	241:292	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	2	41	theme	starch/microcrystalline	260:282	arg1	SC					302:303	ZnO:Eu@SC	295:303	ZnO:Eu@SC	295:303	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	3	42	theme	oils	411:414	arg1	advantages					364:373	the advantages	360:373	the advantages of metal oxides and plant essential oils	360:414	Because of the advantages of metal oxides and plant essential oils, codoping with these can effectively improve the physicochemical and functional properties of composite films.					
36996962	5	43	theme	nano-ZnO	768:775	arg1	release					757:763	good controlled release	741:763	good controlled release of nano-ZnO and Eu in food simulants	741:800	ZnO:Eu@SC exhibited good controlled release of nano-ZnO and Eu in food simulants.					
36996962	2	44	theme	yam	256:258	arg1	cellulose					284:292	a ZnO: eugenol@yam starch/microcrystalline cellulose	241:292	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	2	44	theme	yam	256:258	arg1	SC					302:303	ZnO:Eu@SC	295:303	ZnO:Eu@SC	295:303	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	5	45	theme	Eu	781:782	arg1	release					757:763	good controlled release	741:763	good controlled release of nano-ZnO and Eu in food simulants	741:800	ZnO:Eu@SC exhibited good controlled release of nano-ZnO and Eu in food simulants.					
36996962	0	46	theme	ZnO/eugenol	37:47	arg1	yam					49:51	nano ZnO/eugenol@yam	32:51	nano ZnO/eugenol@yam	32:51	Construction and application of nano ZnO/eugenol@yam starch/microcrystalline cellulose active antibacterial film.					
36996962	7	47	theme	Eu	959:960	arg1	SC					962:963	Eu@SC	959:963	ZnO:Eu@SC	955:963	After loading Eu, the antimicrobial activity of ZnO:Eu@SC was significantly enhanced, resulting in a synergistic antibacterial effect.					
36996962	6	48	dep	diffusion	861:869	arg1	primary					872:878	primary	872:878	primary	872:878	Nano-ZnO and Eu release was controlled by two mechanisms: diffusion (primary) and swelling (secondary).					
36996962	3	49	theme	plant	395:399	arg1	oils					411:414	plant essential oils	395:414	plant essential oils	395:414	Because of the advantages of metal oxides and plant essential oils, codoping with these can effectively improve the physicochemical and functional properties of composite films.					
36996962	0	50	theme	nano	32:35	arg1	yam					49:51	nano ZnO/eugenol@yam	32:51	nano ZnO/eugenol@yam	32:51	Construction and application of nano ZnO/eugenol@yam starch/microcrystalline cellulose active antibacterial film.					
36996962	7	51	theme	@	961:961	arg1	SC					962:963	Eu@SC	959:963	ZnO:Eu@SC	955:963	After loading Eu, the antimicrobial activity of ZnO:Eu@SC was significantly enhanced, resulting in a synergistic antibacterial effect.					
36996962	3	52	theme	essential	401:409	arg1	oils					411:414	plant essential oils	395:414	plant essential oils	395:414	Because of the advantages of metal oxides and plant essential oils, codoping with these can effectively improve the physicochemical and functional properties of composite films.					
36996962	0	53	theme	yam	49:51	arg1	application					17:27	application	17:27	application of nano ZnO/eugenol@yam	17:51	Construction and application of nano ZnO/eugenol@yam starch/microcrystalline cellulose active antibacterial film.					
36996962	0	53	theme	yam	49:51	arg1	Construction					0:11	Construction	0:11	Construction	0:11	Construction and application of nano ZnO/eugenol@yam starch/microcrystalline cellulose active antibacterial film.					
36996962	7	54	theme	ZnO	955:957	arg1	activity					943:950	the antimicrobial activity	925:950	the antimicrobial activity of ZnO:Eu@SC	925:963	After loading Eu, the antimicrobial activity of ZnO:Eu@SC was significantly enhanced, resulting in a synergistic antibacterial effect.					
36996962	7	55	theme	antimicrobial	929:941	arg1	activity					943:950	the antimicrobial activity	925:950	the antimicrobial activity of ZnO:Eu@SC	925:963	After loading Eu, the antimicrobial activity of ZnO:Eu@SC was significantly enhanced, resulting in a synergistic antibacterial effect.					
36996962	2	56	theme	@	301:301	arg1	cellulose					284:292	a ZnO: eugenol@yam starch/microcrystalline cellulose	241:292	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	2	56	theme	@	301:301	arg1	SC					302:303	ZnO:Eu@SC	295:303	ZnO:Eu@SC	295:303	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	0	57	theme	@	48:48	arg1	yam					49:51	nano ZnO/eugenol@yam	32:51	nano ZnO/eugenol@yam	32:51	Construction and application of nano ZnO/eugenol@yam starch/microcrystalline cellulose active antibacterial film.					
36996962	6	58	dep	swelling	885:892	arg1	secondary					895:903	secondary	895:903	secondary	895:903	Nano-ZnO and Eu release was controlled by two mechanisms: diffusion (primary) and swelling (secondary).					
36996962	4	59	theme	barrier	689:695	arg1	properties					697:706	the mechanical and barrier properties	670:706	properties	697:706	The addition of an appropriate amount of nano-ZnO improved the compactness and thermostability, reduced the moisture sensitivity, and enhanced the mechanical and barrier properties of the film.					
36996962	6	60	theme	Eu	816:817	arg1	release					819:825	Eu release	816:825	Eu release	816:825	Nano-ZnO and Eu release was controlled by two mechanisms: diffusion (primary) and swelling (secondary).					
36996962	1	61	theme	study	131:135	arg1	goal					118:121	The goal	114:121	The goal of this study	114:135	The goal of this study was to develop new biocomposite films that can better protect and prolong the shelf life of food.					
36996962	3	62	theme	composite	510:518	arg1	films					520:524	composite films	510:524	composite films	510:524	Because of the advantages of metal oxides and plant essential oils, codoping with these can effectively improve the physicochemical and functional properties of composite films.					
36996962	1	63	theme	shelf	215:219	arg1	life					221:224	the shelf life	211:224	the shelf life of food	211:232	The goal of this study was to develop new biocomposite films that can better protect and prolong the shelf life of food.					
36996962	3	64	theme	functional	485:494	arg1	properties					496:505	the physicochemical and functional properties	461:505	the physicochemical and functional properties of composite films	461:524	Because of the advantages of metal oxides and plant essential oils, codoping with these can effectively improve the physicochemical and functional properties of composite films.					
36996962	10	65	theme	ZnO	1188:1190	arg1	film					1198:1201	the ZnO:Eu@SC film	1184:1201	the ZnO:Eu@SC film	1184:1201	Therefore, the ZnO:Eu@SC film has excellent potential in food active packaging.					
36996962	2	66	theme	ZnO	295:297	arg1	cellulose					284:292	a ZnO: eugenol@yam starch/microcrystalline cellulose	241:292	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	2	66	theme	ZnO	295:297	arg1	SC					302:303	ZnO:Eu@SC	295:303	ZnO:Eu@SC	295:303	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	4	67	theme	amount	558:563	arg1	addition					531:538	The addition	527:538	The addition of an appropriate amount of nano-ZnO	527:575	The addition of an appropriate amount of nano-ZnO improved the compactness and thermostability, reduced the moisture sensitivity, and enhanced the mechanical and barrier properties of the film.					
36996962	0	68	theme	cellulose	77:85	arg1	film					108:111	cellulose active antibacterial film	77:111	cellulose active antibacterial film	77:111	Construction and application of nano ZnO/eugenol@yam starch/microcrystalline cellulose active antibacterial film.					
36996962	5	69	theme	food	787:790	arg1	simulants					792:800	food simulants	787:800	food simulants	787:800	ZnO:Eu@SC exhibited good controlled release of nano-ZnO and Eu in food simulants.					
36996962	7	70	theme	loading	913:919	arg1	Eu					921:922	loading Eu	913:922	loading Eu	913:922	After loading Eu, the antimicrobial activity of ZnO:Eu@SC was significantly enhanced, resulting in a synergistic antibacterial effect.					
36996962	2	71	theme	active	320:325	arg1	film					327:330	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	9	72	theme	ZnO	1117:1119	arg1	film					1127:1130	the ZnO:Eu@SC film	1113:1130	the ZnO:Eu@SC film	1113:1130	In humus, the ZnO:Eu@SC film was effectively degraded into fragments.					
36996962	4	73	theme	nano-ZnO	568:575	arg1	nano-ZnO					568:575	nano-ZnO	568:575	nano-ZnO	568:575	The addition of an appropriate amount of nano-ZnO improved the compactness and thermostability, reduced the moisture sensitivity, and enhanced the mechanical and barrier properties of the film.					
36996962	4	73	theme	nano-ZnO	568:575	arg1	amount					558:563	an appropriate amount	543:563	an appropriate amount of nano-ZnO	543:575	The addition of an appropriate amount of nano-ZnO improved the compactness and thermostability, reduced the moisture sensitivity, and enhanced the mechanical and barrier properties of the film.					
36996962	2	74	theme	antibacterial	306:318	arg1	film					327:330	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film	241:330	Here, a ZnO: eugenol@yam starch/microcrystalline cellulose (ZnO:Eu@SC) antibacterial active film was constructed.					
36996962	1	75	theme	food	229:232	arg1	life					221:224	the shelf life	211:224	the shelf life of food	211:232	The goal of this study was to develop new biocomposite films that can better protect and prolong the shelf life of food.					
35589671	6	0	theme	hyperlipidemic	808:821	arg1	induction					823:831	hyperlipidemic induction	808:831	hyperlipidemic induction with triton WR-1339	808:851	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	3	1	theme	Caatinga	200:207	arg1	species					209:215	a native Caatinga species	191:215	a native Caatinga species	191:215	is described as a native Caatinga species, used by nutritional and medicinal purposes, although there are still few studies and pharmacological data related to this species.					
35589671	5	2	theme	flour	603:607	arg1	composition					588:598	the organic and inorganic composition	562:598	the organic and inorganic composition of flour (MF-Pc)	562:615	It was analyzed the chemical composition of ethanolic extract (EtOH-Pc) by HPLC-DAD-MS/MS, and the organic and inorganic composition of flour (MF-Pc).					
35589671	5	2	theme	flour	603:607	arg1	HPLC-DAD-MS/MS					542:555	HPLC-DAD-MS/MS	542:555	HPLC-DAD-MS/MS	542:555	It was analyzed the chemical composition of ethanolic extract (EtOH-Pc) by HPLC-DAD-MS/MS, and the organic and inorganic composition of flour (MF-Pc).					
35589671	5	2	theme	flour	603:607	arg1	MF-Pc					610:614	MF-Pc	610:614	MF-Pc	610:614	It was analyzed the chemical composition of ethanolic extract (EtOH-Pc) by HPLC-DAD-MS/MS, and the organic and inorganic composition of flour (MF-Pc).					
35589671	9	3	theme	LDL-c	1254:1258	arg1	levels					1236:1241	the lipid levels	1226:1241	the lipid levels of TC, TG, LDL-c and VLDL-c	1226:1269	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	9	4	theme	VLDL-c	1264:1269	arg1	levels					1236:1241	the lipid levels	1226:1241	the lipid levels of TC, TG, LDL-c and VLDL-c	1226:1269	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	6	5	theme	same	790:793	arg1	doses					795:799	the same doses	786:799	the same doses	786:799	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	10	6	theme	hepatic	1472:1478	arg1	steatosis					1480:1488	hepatic steatosis	1472:1488	hepatic steatosis	1472:1488	The results of the histopathological analysis showed that in the group only induced was identified the discrete presence of hepatic steatosis, in 2 animals at the analysis of 24 h, not being visualized in the groups treated with the substances evaluated.					
35589671	6	7	theme	pretreatment	718:729	arg1	treatment					771:779	a single treatment	762:779	a single treatment with the same doses	762:799	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	6	7	theme	pretreatment	718:729	arg1	potential					677:685	the lipid-lowering potential	658:685	the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg)	658:755	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	6	7	theme	pretreatment	718:729	arg1	toxicity					648:655	the acute toxicity	638:655	the acute toxicity	638:655	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	4	8	from	peel	437:440	arg1	mice					461:464	mice	461:464	mice	461:464	This paper aims to evaluate the safety profile and hypolipidemic potential of the fruit peel of this species in mice.					
35589671	10	9	dep	identified	1436:1445	arg1	visualized					1539:1548	visualized	1539:1548	not being visualized in the groups treated with the substances evaluated	1529:1600	The results of the histopathological analysis showed that in the group only induced was identified the discrete presence of hepatic steatosis, in 2 animals at the analysis of 24 h, not being visualized in the groups treated with the substances evaluated.					
35589671	8	10	theme	toxicity	1083:1090	arg1	signs					1074:1078	No clinical signs	1062:1078	No clinical signs of toxicity or death	1062:1099	No clinical signs of toxicity or death were reported in the study.					
35589671	6	11	theme	animal	863:868	arg1	mice					895:898	animal model Swiss Mus musculus mice	863:898	animal model Swiss Mus musculus mice	863:898	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	0	12	theme	Cincinnata	104:113	arg1	Mast					115:118	Passiflora Cincinnata Mast	93:118	Passiflora Cincinnata Mast	93:118	Hypolipidemic potential and safety profile of the ethanolic extract and flour of the peel of Passiflora Cincinnata Mast.					
35589671	8	13	theme	death	1095:1099	arg1	signs					1074:1078	No clinical signs	1062:1078	No clinical signs of toxicity or death	1062:1099	No clinical signs of toxicity or death were reported in the study.					
35589671	10	14	theme	discrete	1451:1458	arg1	presence					1460:1467	the discrete presence	1447:1467	the discrete presence of hepatic steatosis	1447:1488	The results of the histopathological analysis showed that in the group only induced was identified the discrete presence of hepatic steatosis, in 2 animals at the analysis of 24 h, not being visualized in the groups treated with the substances evaluated.					
35589671	11	15	theme	present	1631:1637	arg1	study					1639:1643	the present study	1627:1643	the present study	1627:1643	The results obtained in the present study suggest a hypolipidemic potential of the extract and flour, obtained from the fruit peel of Passiflora cincinnata Mast.					
35589671	9	16	theme	induced	1308:1314	arg1	p < 0.05					1337:1344	p < 0.05	1337:1344	p < 0.05	1337:1344	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	9	16	theme	induced	1308:1314	arg1	mice					1331:1334	the induced hyperlipidemic mice	1304:1334	the induced hyperlipidemic mice (p < 0.05)	1304:1345	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	7	17	theme	antioxidant	1019:1029	arg1	minerals					1031:1038	antioxidant minerals	1019:1038	antioxidant minerals	1019:1038	The presence of flavonoids in the extract was confirmed, mainly C-glycosides, and antioxidant minerals and pectin, in flour.					
35589671	5	18	theme	ethanolic	511:519	arg1	EtOH-Pc					530:536	EtOH-Pc	530:536	EtOH-Pc	530:536	It was analyzed the chemical composition of ethanolic extract (EtOH-Pc) by HPLC-DAD-MS/MS, and the organic and inorganic composition of flour (MF-Pc).					
35589671	5	18	theme	ethanolic	511:519	arg1	extract					521:527	ethanolic extract	511:527	ethanolic extract (EtOH-Pc)	511:537	It was analyzed the chemical composition of ethanolic extract (EtOH-Pc) by HPLC-DAD-MS/MS, and the organic and inorganic composition of flour (MF-Pc).					
35589671	4	19	theme	safety	381:386	arg1	profile					388:394	safety profile	381:394	safety profile	381:394	This paper aims to evaluate the safety profile and hypolipidemic potential of the fruit peel of this species in mice.					
35589671	9	20	theme	hyperlipidemia	1136:1149	arg1	model					1157:1161	the hyperlipidemia study model	1132:1161	the hyperlipidemia study model used	1132:1166	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	6	21	theme	histopathological	909:925	arg1	analysis					927:934	histopathological analysis	909:934	histopathological analysis	909:934	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	0	22	theme	extract	60:66	arg1	flour					72:76	flour	72:76	flour of the peel of Passiflora Cincinnata Mast	72:118	Hypolipidemic potential and safety profile of the ethanolic extract and flour of the peel of Passiflora Cincinnata Mast.					
35589671	0	22	theme	extract	60:66	arg1	potential					14:22	Hypolipidemic potential	0:22	Hypolipidemic potential	0:22	Hypolipidemic potential and safety profile of the ethanolic extract and flour of the peel of Passiflora Cincinnata Mast.					
35589671	0	22	theme	extract	60:66	arg1	profile					35:41	safety profile	28:41	safety profile	28:41	Hypolipidemic potential and safety profile of the ethanolic extract and flour of the peel of Passiflora Cincinnata Mast.					
35589671	3	23	theme	few	287:289	arg1	studies					291:297	few studies	287:297	few studies	287:297	is described as a native Caatinga species, used by nutritional and medicinal purposes, although there are still few studies and pharmacological data related to this species.					
35589671	9	24	theme	analyzed	1173:1180	arg1	substances					1182:1191	the analyzed substances	1169:1191	the analyzed substances	1169:1191	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	6	25	theme	Swiss	876:880	arg1	mice					895:898	animal model Swiss Mus musculus mice	863:898	animal model Swiss Mus musculus mice	863:898	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	4	26	theme	peel	437:440	arg1	profile					388:394	safety profile	381:394	safety profile	381:394	This paper aims to evaluate the safety profile and hypolipidemic potential of the fruit peel of this species in mice.					
35589671	4	26	theme	peel	437:440	arg1	potential					414:422	hypolipidemic potential	400:422	hypolipidemic potential	400:422	This paper aims to evaluate the safety profile and hypolipidemic potential of the fruit peel of this species in mice.					
35589671	6	27	theme	acute	642:646	arg1	toxicity					648:655	the acute toxicity	638:655	the acute toxicity	638:655	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	11	28	theme	flour	1698:1702	arg1	potential					1669:1677	a hypolipidemic potential	1653:1677	a hypolipidemic potential of the extract and flour, obtained from the fruit peel of Passiflora cincinnata Mast	1653:1762	The results obtained in the present study suggest a hypolipidemic potential of the extract and flour, obtained from the fruit peel of Passiflora cincinnata Mast.					
35589671	0	29	theme	Hypolipidemic	0:12	arg1	potential					14:22	Hypolipidemic potential	0:22	Hypolipidemic potential	0:22	Hypolipidemic potential and safety profile of the ethanolic extract and flour of the peel of Passiflora Cincinnata Mast.					
35589671	9	30	from	doses	1201:1205	arg1	substances					1182:1191	the analyzed substances	1169:1191	the analyzed substances	1169:1191	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	4	31	from	species	450:456	arg1	mice					461:464	mice	461:464	mice	461:464	This paper aims to evaluate the safety profile and hypolipidemic potential of the fruit peel of this species in mice.					
35589671	7	32	attach	presence	941:948	arg2	flavonoids					953:962	flavonoids	953:962	flavonoids	953:962	The presence of flavonoids in the extract was confirmed, mainly C-glycosides, and antioxidant minerals and pectin, in flour.					
35589671	7	32	attach	presence	941:948	arg1	extract					971:977	the extract	967:977	the extract	967:977	The presence of flavonoids in the extract was confirmed, mainly C-glycosides, and antioxidant minerals and pectin, in flour.					
35589671	6	33	with	toxicity	648:655	arg1	doses					795:799	the same doses	786:799	the same doses	786:799	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	2	34	theme	cincinnata	158:167	arg1	Mast					169:172	Passiflora cincinnata Mast	147:172	Passiflora cincinnata Mast.	147:173	Passiflora cincinnata Mast.					
35589671	6	35	theme	samples	696:702	arg1	treatment					771:779	a single treatment	762:779	a single treatment with the same doses	762:799	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	6	35	theme	samples	696:702	arg1	potential					677:685	the lipid-lowering potential	658:685	the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg)	658:755	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	6	35	theme	samples	696:702	arg1	toxicity					648:655	the acute toxicity	638:655	the acute toxicity	638:655	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	11	36	theme	hypolipidemic	1655:1667	arg1	potential					1669:1677	a hypolipidemic potential	1653:1677	a hypolipidemic potential of the extract and flour, obtained from the fruit peel of Passiflora cincinnata Mast	1653:1762	The results obtained in the present study suggest a hypolipidemic potential of the extract and flour, obtained from the fruit peel of Passiflora cincinnata Mast.					
35589671	9	37	theme	lipid	1230:1234	arg1	levels					1236:1241	the lipid levels	1226:1241	the lipid levels of TC, TG, LDL-c and VLDL-c	1226:1269	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	9	38	from	levels	1294:1299	arg1	p < 0.05					1337:1344	p < 0.05	1337:1344	p < 0.05	1337:1344	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	9	38	from	levels	1294:1299	arg1	mice					1331:1334	the induced hyperlipidemic mice	1304:1334	the induced hyperlipidemic mice (p < 0.05)	1304:1345	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	10	39	theme	analysis	1385:1392	arg1	results					1352:1358	The results	1348:1358	The results of the histopathological analysis	1348:1392	The results of the histopathological analysis showed that in the group only induced was identified the discrete presence of hepatic steatosis, in 2 animals at the analysis of 24 h, not being visualized in the groups treated with the substances evaluated.					
35589671	6	40	theme	lipid-lowering	662:675	arg1	potential					677:685	the lipid-lowering potential	658:685	the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg)	658:755	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	11	41	theme	extract	1686:1692	arg1	potential					1669:1677	a hypolipidemic potential	1653:1677	a hypolipidemic potential of the extract and flour, obtained from the fruit peel of Passiflora cincinnata Mast	1653:1762	The results obtained in the present study suggest a hypolipidemic potential of the extract and flour, obtained from the fruit peel of Passiflora cincinnata Mast.					
35589671	4	42	dep	profile	388:394	arg1	the					377:379	the	377:379	the	377:379	This paper aims to evaluate the safety profile and hypolipidemic potential of the fruit peel of this species in mice.					
35589671	4	43	from	mice	461:464	arg1	peel					437:440	the fruit peel	427:440	the fruit peel of this species in mice	427:464	This paper aims to evaluate the safety profile and hypolipidemic potential of the fruit peel of this species in mice.					
35589671	3	44	theme	native	193:198	arg1	species					209:215	a native Caatinga species	191:215	a native Caatinga species	191:215	is described as a native Caatinga species, used by nutritional and medicinal purposes, although there are still few studies and pharmacological data related to this species.					
35589671	6	45	with	potential	677:685	arg1	doses					795:799	the same doses	786:799	the same doses	786:799	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	10	46	theme	24 h	1523:1526	arg1	analysis					1511:1518	the analysis	1507:1518	the analysis of 24 h	1507:1526	The results of the histopathological analysis showed that in the group only induced was identified the discrete presence of hepatic steatosis, in 2 animals at the analysis of 24 h, not being visualized in the groups treated with the substances evaluated.					
35589671	5	47	theme	organic	566:572	arg1	composition					588:598	the organic and inorganic composition	562:598	the organic and inorganic composition of flour (MF-Pc)	562:615	It was analyzed the chemical composition of ethanolic extract (EtOH-Pc) by HPLC-DAD-MS/MS, and the organic and inorganic composition of flour (MF-Pc).					
35589671	5	47	theme	organic	566:572	arg1	MF-Pc					610:614	MF-Pc	610:614	MF-Pc	610:614	It was analyzed the chemical composition of ethanolic extract (EtOH-Pc) by HPLC-DAD-MS/MS, and the organic and inorganic composition of flour (MF-Pc).					
35589671	11	48	dep	Passiflora	1737:1746	arg1	Mast					1759:1762	Mast	1759:1762	Passiflora cincinnata Mast	1737:1762	The results obtained in the present study suggest a hypolipidemic potential of the extract and flour, obtained from the fruit peel of Passiflora cincinnata Mast.					
35589671	11	48	dep	Passiflora	1737:1746	arg1	cincinnata					1748:1757	Passiflora cincinnata Mast	1737:1762	Passiflora cincinnata Mast	1737:1762	The results obtained in the present study suggest a hypolipidemic potential of the extract and flour, obtained from the fruit peel of Passiflora cincinnata Mast.					
35589671	3	49	theme	nutritional	226:236	arg1	purposes					252:259	nutritional and medicinal purposes	226:259	nutritional and medicinal purposes	226:259	is described as a native Caatinga species, used by nutritional and medicinal purposes, although there are still few studies and pharmacological data related to this species.					
35589671	6	50	dep	pretreatment	718:729	arg1	oral					732:735	oral	732:735	oral	732:735	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	5	51	theme	inorganic	578:586	arg1	composition					588:598	the organic and inorganic composition	562:598	the organic and inorganic composition of flour (MF-Pc)	562:615	It was analyzed the chemical composition of ethanolic extract (EtOH-Pc) by HPLC-DAD-MS/MS, and the organic and inorganic composition of flour (MF-Pc).					
35589671	5	51	theme	inorganic	578:586	arg1	MF-Pc					610:614	MF-Pc	610:614	MF-Pc	610:614	It was analyzed the chemical composition of ethanolic extract (EtOH-Pc) by HPLC-DAD-MS/MS, and the organic and inorganic composition of flour (MF-Pc).					
35589671	8	52	theme	clinical	1065:1072	arg1	signs					1074:1078	No clinical signs	1062:1078	No clinical signs of toxicity or death	1062:1099	No clinical signs of toxicity or death were reported in the study.					
35589671	6	53	theme	model	870:874	arg1	mice					895:898	animal model Swiss Mus musculus mice	863:898	animal model Swiss Mus musculus mice	863:898	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	6	54	dep	oral	732:735	arg1	200 mg/kg					746:754	200 mg/kg	746:754	200 mg/kg	746:754	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	6	54	dep	oral	732:735	arg1	100					738:740	100	738:740	100	738:740	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	0	55	theme	Mast	115:118	arg1	peel					85:88	the peel	81:88	the peel of Passiflora Cincinnata Mast	81:118	Hypolipidemic potential and safety profile of the ethanolic extract and flour of the peel of Passiflora Cincinnata Mast.					
35589671	7	56	theme	flavonoids	953:962	arg1	presence					941:948	The presence	937:948	The presence of flavonoids in the extract	937:977	The presence of flavonoids in the extract was confirmed, mainly C-glycosides, and antioxidant minerals and pectin, in flour.					
35589671	9	57	theme	HDL-c	1288:1292	arg1	levels					1294:1299	the HDL-c levels	1284:1299	the HDL-c levels in the induced hyperlipidemic mice (p < 0.05)	1284:1345	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	3	58	theme	medicinal	242:250	arg1	purposes					252:259	nutritional and medicinal purposes	226:259	nutritional and medicinal purposes	226:259	is described as a native Caatinga species, used by nutritional and medicinal purposes, although there are still few studies and pharmacological data related to this species.					
35589671	4	59	theme	fruit	431:435	arg1	peel					437:440	the fruit peel	427:440	the fruit peel of this species in mice	427:464	This paper aims to evaluate the safety profile and hypolipidemic potential of the fruit peel of this species in mice.					
35589671	11	60	theme	Passiflora	1737:1746	arg1	peel					1729:1732	the fruit peel	1719:1732	the fruit peel of Passiflora cincinnata Mast	1719:1762	The results obtained in the present study suggest a hypolipidemic potential of the extract and flour, obtained from the fruit peel of Passiflora cincinnata Mast.					
35589671	9	61	theme	hyperlipidemic	1316:1329	arg1	p < 0.05					1337:1344	p < 0.05	1337:1344	p < 0.05	1337:1344	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	9	61	theme	hyperlipidemic	1316:1329	arg1	mice					1331:1334	the induced hyperlipidemic mice	1304:1334	the induced hyperlipidemic mice (p < 0.05)	1304:1345	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	5	62	theme	extract	521:527	arg1	composition					496:506	the chemical composition	483:506	the chemical composition of ethanolic extract (EtOH-Pc)	483:537	It was analyzed the chemical composition of ethanolic extract (EtOH-Pc) by HPLC-DAD-MS/MS, and the organic and inorganic composition of flour (MF-Pc).					
35589671	4	63	theme	hypolipidemic	400:412	arg1	potential					414:422	hypolipidemic potential	400:422	hypolipidemic potential	400:422	This paper aims to evaluate the safety profile and hypolipidemic potential of the fruit peel of this species in mice.					
35589671	9	64	theme	study	1151:1155	arg1	model					1157:1161	the hyperlipidemia study model	1132:1161	the hyperlipidemia study model used	1132:1166	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	3	65	theme	pharmacological	303:317	arg1	data					319:322	pharmacological data	303:322	pharmacological data related to this species	303:346	is described as a native Caatinga species, used by nutritional and medicinal purposes, although there are still few studies and pharmacological data related to this species.					
35589671	6	66	dep	Mus	882:884	arg1	musculus					886:893	musculus	886:893	musculus	886:893	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	5	67	theme	chemical	487:494	arg1	composition					496:506	the chemical composition	483:506	the chemical composition of ethanolic extract (EtOH-Pc)	483:537	It was analyzed the chemical composition of ethanolic extract (EtOH-Pc) by HPLC-DAD-MS/MS, and the organic and inorganic composition of flour (MF-Pc).					
35589671	0	68	theme	peel	85:88	arg1	flour					72:76	flour	72:76	flour of the peel of Passiflora Cincinnata Mast	72:118	Hypolipidemic potential and safety profile of the ethanolic extract and flour of the peel of Passiflora Cincinnata Mast.					
35589671	0	68	theme	peel	85:88	arg1	potential					14:22	Hypolipidemic potential	0:22	Hypolipidemic potential	0:22	Hypolipidemic potential and safety profile of the ethanolic extract and flour of the peel of Passiflora Cincinnata Mast.					
35589671	0	68	theme	peel	85:88	arg1	profile					35:41	safety profile	28:41	safety profile	28:41	Hypolipidemic potential and safety profile of the ethanolic extract and flour of the peel of Passiflora Cincinnata Mast.					
35589671	6	69	theme	Mus	882:884	arg1	mice					895:898	animal model Swiss Mus musculus mice	863:898	animal model Swiss Mus musculus mice	863:898	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	0	70	theme	Passiflora	93:102	arg1	Mast					115:118	Passiflora Cincinnata Mast	93:118	Passiflora Cincinnata Mast	93:118	Hypolipidemic potential and safety profile of the ethanolic extract and flour of the peel of Passiflora Cincinnata Mast.					
35589671	3	71	theme	related	324:330	arg1	data					319:322	pharmacological data	303:322	pharmacological data related to this species	303:346	is described as a native Caatinga species, used by nutritional and medicinal purposes, although there are still few studies and pharmacological data related to this species.					
35589671	4	72	theme	species	450:456	arg1	peel					437:440	the fruit peel	427:440	the fruit peel of this species in mice	427:464	This paper aims to evaluate the safety profile and hypolipidemic potential of the fruit peel of this species in mice.					
35589671	10	73	theme	steatosis	1480:1488	arg1	presence					1460:1467	the discrete presence	1447:1467	the discrete presence of hepatic steatosis	1447:1488	The results of the histopathological analysis showed that in the group only induced was identified the discrete presence of hepatic steatosis, in 2 animals at the analysis of 24 h, not being visualized in the groups treated with the substances evaluated.					
35589671	6	74	with	treatment	771:779	arg1	doses					795:799	the same doses	786:799	the same doses	786:799	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	1	75	dep	mice	141:144	arg1	Passifloraceae					122:135	Passifloraceae	122:135	Passifloraceae	122:135	(Passifloraceae) in mice.					
35589671	0	76	theme	safety	28:33	arg1	profile					35:41	safety profile	28:41	safety profile	28:41	Hypolipidemic potential and safety profile of the ethanolic extract and flour of the peel of Passiflora Cincinnata Mast.					
35589671	11	77	dep	extract	1686:1692	arg1	the					1682:1684	the	1682:1684	the	1682:1684	The results obtained in the present study suggest a hypolipidemic potential of the extract and flour, obtained from the fruit peel of Passiflora cincinnata Mast.					
35589671	6	78	theme	single	764:769	arg1	treatment					771:779	a single treatment	762:779	a single treatment with the same doses	762:799	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	2	79	theme	Passiflora	147:156	arg1	Mast					169:172	Passiflora cincinnata Mast	147:172	Passiflora cincinnata Mast.	147:173	Passiflora cincinnata Mast.					
35589671	11	80	theme	fruit	1723:1727	arg1	peel					1729:1732	the fruit peel	1719:1732	the fruit peel of Passiflora cincinnata Mast	1719:1762	The results obtained in the present study suggest a hypolipidemic potential of the extract and flour, obtained from the fruit peel of Passiflora cincinnata Mast.					
35589671	0	81	theme	ethanolic	50:58	arg1	extract					60:66	the ethanolic extract	46:66	the ethanolic extract	46:66	Hypolipidemic potential and safety profile of the ethanolic extract and flour of the peel of Passiflora Cincinnata Mast.					
35589671	6	82	with	induction	823:831	arg1	triton					838:843	triton WR-1339	838:851	triton WR-1339	838:851	Also were evaluated the acute toxicity, the lipid-lowering potential of these samples, through of a pretreatment (oral: 100 and 200 mg/kg), and a single treatment with the same doses, after hyperlipidemic induction with triton WR-1339, using as animal model Swiss Mus musculus mice, besides histopathological analysis.					
35589671	9	83	theme	TC	1246:1247	arg1	levels					1236:1241	the lipid levels	1226:1241	the lipid levels of TC, TG, LDL-c and VLDL-c	1226:1269	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
35589671	7	84	from	presence	941:948	arg1	extract					971:977	the extract	967:977	the extract	967:977	The presence of flavonoids in the extract was confirmed, mainly C-glycosides, and antioxidant minerals and pectin, in flour.					
35589671	10	85	theme	histopathological	1367:1383	arg1	analysis					1385:1392	the histopathological analysis	1363:1392	the histopathological analysis	1363:1392	The results of the histopathological analysis showed that in the group only induced was identified the discrete presence of hepatic steatosis, in 2 animals at the analysis of 24 h, not being visualized in the groups treated with the substances evaluated.					
35589671	9	86	theme	TG	1250:1251	arg1	levels					1236:1241	the lipid levels	1226:1241	the lipid levels of TC, TG, LDL-c and VLDL-c	1226:1269	In the hyperlipidemia study model used, the analyzed substances, at all doses, notably decreased the lipid levels of TC, TG, LDL-c and VLDL-c and increase the HDL-c levels in the induced hyperlipidemic mice (p < 0.05).					
36137521	0	0	theme	wound	88:92	arg1	applications					103:114	wound dressing applications	88:114	wound dressing applications	88:114	Development of antimicrobial nanocomposite scaffolds via loading CZTSe quantum dots for wound dressing applications.					
36137521	7	1	theme	potential	1326:1334	arg1	applications					1336:1347	great potential applications	1320:1347	great potential applications	1320:1347	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	4	2	theme	different	662:670	arg1	concentrations					672:685	different concentrations	662:685	different concentrations of the synthesized nanoparticles	662:718	Polymer-based nanocomposites with different concentrations of the synthesized nanoparticles were produced by the solvent casting method.					
36137521	2	3	theme	healing	529:535	arg1	process					537:543	the wound healing process	519:543	the wound healing process	519:543	For this purpose, chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process were produced.					
36137521	2	4	theme	composite	367:375	arg1	membranes					377:385	chitosan-polyvinyl alcohol composite membranes	340:385	chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process	340:543	For this purpose, chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process were produced.					
36137521	7	5	theme	strong	1452:1457	arg1	properties					1473:1482	strong antibacterial properties	1452:1482	strong antibacterial properties	1452:1482	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	7	6	theme	antibacterial	1459:1471	arg1	properties					1473:1482	strong antibacterial properties	1452:1482	strong antibacterial properties	1452:1482	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	4	7	with	nanocomposites	642:655	arg1	concentrations					672:685	different concentrations	662:685	different concentrations of the synthesized nanoparticles	662:718	Polymer-based nanocomposites with different concentrations of the synthesized nanoparticles were produced by the solvent casting method.					
36137521	6	8	theme	QDs	1132:1134	arg1	concentration					1136:1148	a QDs concentration	1130:1148	a QDs concentration of 3.3% by weight	1130:1166	The results show that the preparation of composite scaffolds at a QDs concentration of 3.3% by weight has the best antimicrobial activity.					
36137521	0	9	theme	dressing	94:101	arg1	applications					103:114	wound dressing applications	88:114	wound dressing applications	88:114	Development of antimicrobial nanocomposite scaffolds via loading CZTSe quantum dots for wound dressing applications.					
36137521	6	10	contain	has	1168:1170	arg1	preparation					1092:1102	the preparation	1088:1102	the preparation of composite scaffolds at a QDs concentration of 3.3% by weight	1088:1166	The results show that the preparation of composite scaffolds at a QDs concentration of 3.3% by weight has the best antimicrobial activity.					
36137521	6	10	contain	has	1168:1170	arg2	activity					1195:1202	the best antimicrobial activity	1172:1202	the best antimicrobial activity	1172:1202	The results show that the preparation of composite scaffolds at a QDs concentration of 3.3% by weight has the best antimicrobial activity.					
36137521	3	11	theme	hydrothermal	603:614	arg1	conditions					616:625	hydrothermal conditions	603:625	hydrothermal conditions	603:625	CZTSe QDs particles were synthesized under hydrothermal conditions.					
36137521	1	12	theme	wound	270:274	arg1	healing					276:282	the wound healing	266:282	the wound healing process	266:290	The antimicrobial properties of scaffolds designed for use in wound healing are accepted as an important factor in the healing process to accelerate the wound healing process without causing inflammation.					
36137521	5	13	theme	membranes	859:867	arg1	characterizations					814:830	detailed physicochemical and morphological characterizations	771:830	detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes	771:867	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	2	14	theme	alcohol	359:365	arg1	membranes					377:385	chitosan-polyvinyl alcohol composite membranes	340:385	chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process	340:543	For this purpose, chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process were produced.					
36137521	7	15	theme	process	1291:1297	arg1	result					1262:1267	a result	1260:1267	a result of an easy production process	1260:1297	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	7	15	theme	process	1291:1297	arg1	membranes					1224:1232	Composite scaffold membranes	1205:1232	Composite scaffold membranes	1205:1232	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	1	16	theme	wound	179:183	arg1	healing					185:191	wound healing	179:191	wound healing	179:191	The antimicrobial properties of scaffolds designed for use in wound healing are accepted as an important factor in the healing process to accelerate the wound healing process without causing inflammation.					
36137521	5	17	theme	gram-positive	952:964	arg1	strains					1004:1010	bacterial and yeast strains	984:1010	strains	1004:1010	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	4	18	theme	casting	749:755	arg1	method					757:762	the solvent casting method	737:762	the solvent casting method	737:762	Polymer-based nanocomposites with different concentrations of the synthesized nanoparticles were produced by the solvent casting method.					
36137521	5	19	theme	cell	899:902	arg1	viability					904:912	cell viability	899:912	cell viability	899:912	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	7	20	theme	high	1411:1414	arg1	properties					1427:1436	their high mechanical properties	1405:1436	their high mechanical properties	1405:1436	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	7	21	theme	dressing	1380:1387	arg1	material					1389:1396	wound dressing material	1374:1396	wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity	1374:1499	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	7	22	theme	due	1398:1400	arg1	material					1389:1396	wound dressing material	1374:1396	wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity	1374:1499	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	5	23	theme	L929	1017:1020	arg1	lines					1045:1049	L929 mouse fibroblast cells lines	1017:1049	L929 mouse fibroblast cells lines	1017:1049	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	2	24	theme	cytocompatible	480:493	arg1	biomaterial					495:505	an antibacterial and cytocompatible biomaterial to regulate the wound healing process	459:543	an antibacterial and cytocompatible biomaterial to regulate the wound healing process	459:543	For this purpose, chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process were produced.					
36137521	1	25	theme	healing	276:282	arg1	process					284:290	the wound healing process	266:290	the wound healing process	266:290	The antimicrobial properties of scaffolds designed for use in wound healing are accepted as an important factor in the healing process to accelerate the wound healing process without causing inflammation.					
36137521	6	26	from	concentration	1136:1148	arg1	preparation					1092:1102	the preparation	1088:1102	the preparation of composite scaffolds at a QDs concentration of 3.3% by weight	1088:1166	The results show that the preparation of composite scaffolds at a QDs concentration of 3.3% by weight has the best antimicrobial activity.					
36137521	2	27	theme	Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum	399:437	arg1	dots					439:442	Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots	399:442	Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs)	399:454	For this purpose, chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process were produced.					
36137521	2	27	theme	Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum	399:437	arg1	QDs					451:453	CZTSe QDs	445:453	CZTSe QDs	445:453	For this purpose, chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process were produced.					
36137521	0	28	theme	nanocomposite	29:41	arg1	Development					0:10	Development	0:10	Development of antimicrobial nanocomposite	0:41	Development of antimicrobial nanocomposite scaffolds via loading CZTSe quantum dots for wound dressing applications.					
36137521	5	29	theme	yeast	998:1002	arg1	strains					1004:1010	bacterial and yeast strains	984:1010	strains	1004:1010	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	7	30	theme	Composite	1205:1213	arg1	membranes					1224:1232	Composite scaffold membranes	1205:1232	Composite scaffold membranes	1205:1232	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	7	30	theme	Composite	1205:1213	arg1	result					1262:1267	a result	1260:1267	a result of an easy production process	1260:1297	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	7	31	theme	wound	1374:1378	arg1	material					1389:1396	wound dressing material	1374:1396	wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity	1374:1499	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	5	32	theme	bacterial	984:992	arg1	strains					1004:1010	bacterial and yeast strains	984:1010	strains	1004:1010	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	0	33	theme	antimicrobial	15:27	arg1	nanocomposite					29:41	antimicrobial nanocomposite	15:41	antimicrobial nanocomposite	15:41	Development of antimicrobial nanocomposite scaffolds via loading CZTSe quantum dots for wound dressing applications.					
36137521	6	34	theme	composite	1107:1115	arg1	scaffolds					1117:1125	composite scaffolds	1107:1125	composite scaffolds	1107:1125	The results show that the preparation of composite scaffolds at a QDs concentration of 3.3% by weight has the best antimicrobial activity.					
36137521	7	35	theme	scaffold	1215:1222	arg1	membranes					1224:1232	Composite scaffold membranes	1205:1232	Composite scaffold membranes	1205:1232	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	7	35	theme	scaffold	1215:1222	arg1	result					1262:1267	a result	1260:1267	a result of an easy production process	1260:1297	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	3	36	theme	CZTSe	560:564	arg1	particles					570:578	CZTSe QDs particles	560:578	CZTSe QDs particles	560:578	CZTSe QDs particles were synthesized under hydrothermal conditions.					
36137521	7	37	theme	easy	1275:1278	arg1	process					1291:1297	an easy production process	1272:1297	an easy production process	1272:1297	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	6	38	theme	best	1176:1179	arg1	activity					1195:1202	the best antimicrobial activity	1172:1202	the best antimicrobial activity	1172:1202	The results show that the preparation of composite scaffolds at a QDs concentration of 3.3% by weight has the best antimicrobial activity.					
36137521	2	39	theme	wound	523:527	arg1	healing					529:535	the wound healing	519:535	the wound healing process	519:543	For this purpose, chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process were produced.					
36137521	4	40	theme	Polymer-based	628:640	arg1	nanocomposites					642:655	Polymer-based nanocomposites	628:655	Polymer-based nanocomposites with different concentrations of the synthesized nanoparticles	628:718	Polymer-based nanocomposites with different concentrations of the synthesized nanoparticles were produced by the solvent casting method.					
36137521	5	41	theme	CZTSe	835:839	arg1	QDs					841:843	CZTSe QDs	835:843	CZTSe QDs	835:843	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	4	42	theme	solvent	741:747	arg1	method					757:762	the solvent casting method	737:762	the solvent casting method	737:762	Polymer-based nanocomposites with different concentrations of the synthesized nanoparticles were produced by the solvent casting method.					
36137521	5	43	theme	mouse	1022:1026	arg1	lines					1045:1049	L929 mouse fibroblast cells lines	1017:1049	L929 mouse fibroblast cells lines	1017:1049	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	1	44	theme	important	212:220	arg1	properties					135:144	The antimicrobial properties	117:144	The antimicrobial properties of scaffolds designed for use in wound healing	117:191	The antimicrobial properties of scaffolds designed for use in wound healing are accepted as an important factor in the healing process to accelerate the wound healing process without causing inflammation.					
36137521	1	44	theme	important	212:220	arg1	factor					222:227	an important factor	209:227	an important factor in the healing process	209:250	The antimicrobial properties of scaffolds designed for use in wound healing are accepted as an important factor in the healing process to accelerate the wound healing process without causing inflammation.					
36137521	2	45	theme	CZTSe	445:449	arg1	dots					439:442	Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots	399:442	Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs)	399:454	For this purpose, chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process were produced.					
36137521	2	45	theme	CZTSe	445:449	arg1	QDs					451:453	CZTSe QDs	445:453	CZTSe QDs	445:453	For this purpose, chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process were produced.					
36137521	5	46	theme	QDs	841:843	arg1	characterizations					814:830	detailed physicochemical and morphological characterizations	771:830	detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes	771:867	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	5	47	theme	composite	849:857	arg1	membranes					859:867	composite membranes	849:867	composite membranes	849:867	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	6	48	theme	%	1156:1156	arg1	concentration					1136:1148	a QDs concentration	1130:1148	a QDs concentration of 3.3% by weight	1130:1166	The results show that the preparation of composite scaffolds at a QDs concentration of 3.3% by weight has the best antimicrobial activity.					
36137521	7	49	theme	mechanical	1416:1425	arg1	properties					1427:1436	their high mechanical properties	1405:1436	their high mechanical properties	1405:1436	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	0	50	theme	CZTSe	65:69	arg1	dots					79:82	loading CZTSe quantum dots	57:82	loading CZTSe quantum dots for wound dressing applications	57:114	Development of antimicrobial nanocomposite scaffolds via loading CZTSe quantum dots for wound dressing applications.					
36137521	5	51	theme	detailed	771:778	arg1	characterizations					814:830	detailed physicochemical and morphological characterizations	771:830	detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes	771:867	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	6	52	theme	scaffolds	1117:1125	arg1	preparation					1092:1102	the preparation	1088:1102	the preparation of composite scaffolds at a QDs concentration of 3.3% by weight	1088:1166	The results show that the preparation of composite scaffolds at a QDs concentration of 3.3% by weight has the best antimicrobial activity.					
36137521	1	53	from	factor	222:227	arg1	process					244:250	the healing process	232:250	the healing process	232:250	The antimicrobial properties of scaffolds designed for use in wound healing are accepted as an important factor in the healing process to accelerate the wound healing process without causing inflammation.					
36137521	1	54	theme	antimicrobial	121:133	arg1	properties					135:144	The antimicrobial properties	117:144	The antimicrobial properties of scaffolds designed for use in wound healing	117:191	The antimicrobial properties of scaffolds designed for use in wound healing are accepted as an important factor in the healing process to accelerate the wound healing process without causing inflammation.					
36137521	1	54	theme	antimicrobial	121:133	arg1	factor					222:227	an important factor	209:227	an important factor in the healing process	209:250	The antimicrobial properties of scaffolds designed for use in wound healing are accepted as an important factor in the healing process to accelerate the wound healing process without causing inflammation.					
36137521	0	55	theme	loading	57:63	arg1	dots					79:82	loading CZTSe quantum dots	57:82	loading CZTSe quantum dots for wound dressing applications	57:114	Development of antimicrobial nanocomposite scaffolds via loading CZTSe quantum dots for wound dressing applications.					
36137521	5	56	theme	physicochemical	780:794	arg1	characterizations					814:830	detailed physicochemical and morphological characterizations	771:830	detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes	771:867	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	7	57	theme	great	1320:1324	arg1	applications					1336:1347	great potential applications	1320:1347	great potential applications	1320:1347	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	2	58	theme	chitosan-polyvinyl	340:357	arg1	membranes					377:385	chitosan-polyvinyl alcohol composite membranes	340:385	chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process	340:543	For this purpose, chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process were produced.					
36137521	7	59	theme	production	1280:1289	arg1	process					1291:1297	an easy production process	1272:1297	an easy production process	1272:1297	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	7	60	contain	have	1315:1318	arg1	result					1262:1267	a result	1260:1267	a result of an easy production process	1260:1297	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	7	60	contain	have	1315:1318	arg1	membranes					1224:1232	Composite scaffold membranes	1205:1232	Composite scaffold membranes	1205:1232	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	7	60	contain	have	1315:1318	arg2	applications					1336:1347	great potential applications	1320:1347	great potential applications	1320:1347	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	4	61	theme	synthesized	694:704	arg1	nanoparticles					706:718	the synthesized nanoparticles	690:718	the synthesized nanoparticles	690:718	Polymer-based nanocomposites with different concentrations of the synthesized nanoparticles were produced by the solvent casting method.					
36137521	5	62	theme	morphological	800:812	arg1	characterizations					814:830	detailed physicochemical and morphological characterizations	771:830	detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes	771:867	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	5	63	theme	gram-negative	970:982	arg1	strains					1004:1010	bacterial and yeast strains	984:1010	strains	1004:1010	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	1	64	theme	scaffolds	149:157	arg1	properties					135:144	The antimicrobial properties	117:144	The antimicrobial properties of scaffolds designed for use in wound healing	117:191	The antimicrobial properties of scaffolds designed for use in wound healing are accepted as an important factor in the healing process to accelerate the wound healing process without causing inflammation.					
36137521	1	64	theme	scaffolds	149:157	arg1	factor					222:227	an important factor	209:227	an important factor in the healing process	209:250	The antimicrobial properties of scaffolds designed for use in wound healing are accepted as an important factor in the healing process to accelerate the wound healing process without causing inflammation.					
36137521	5	65	theme	antibacterial	870:882	arg1	activities					884:893	antibacterial activities	870:893	antibacterial activities	870:893	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	6	66	theme	antimicrobial	1181:1193	arg1	activity					1195:1202	the best antimicrobial activity	1172:1202	the best antimicrobial activity	1172:1202	The results show that the preparation of composite scaffolds at a QDs concentration of 3.3% by weight has the best antimicrobial activity.					
36137521	5	67	theme	fibroblast	1028:1037	arg1	lines					1045:1049	L929 mouse fibroblast cells lines	1017:1049	L929 mouse fibroblast cells lines	1017:1049	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	7	68	theme	tissue	1352:1357	arg1	engineering					1359:1369	tissue engineering	1352:1369	tissue engineering	1352:1369	Composite scaffold membranes, which can be obtained as a result of an easy production process, are thought to have great potential applications in tissue engineering as wound dressing material due to their high mechanical properties, wettability, strong antibacterial properties and non-toxicity.					
36137521	1	69	theme	healing	236:242	arg1	process					244:250	the healing process	232:250	the healing process	232:250	The antimicrobial properties of scaffolds designed for use in wound healing are accepted as an important factor in the healing process to accelerate the wound healing process without causing inflammation.					
36137521	0	70	theme	quantum	71:77	arg1	dots					79:82	loading CZTSe quantum dots	57:82	loading CZTSe quantum dots for wound dressing applications	57:114	Development of antimicrobial nanocomposite scaffolds via loading CZTSe quantum dots for wound dressing applications.					
36137521	2	71	theme	antibacterial	462:474	arg1	biomaterial					495:505	an antibacterial and cytocompatible biomaterial to regulate the wound healing process	459:543	an antibacterial and cytocompatible biomaterial to regulate the wound healing process	459:543	For this purpose, chitosan-polyvinyl alcohol composite membranes loaded with Cu<sub>2</sub>ZnSnSe<sub>4</sub>quantum dots (CZTSe QDs) as an antibacterial and cytocompatible biomaterial to regulate the wound healing process were produced.					
36137521	5	72	theme	cells	1039:1043	arg1	lines					1045:1049	L929 mouse fibroblast cells lines	1017:1049	L929 mouse fibroblast cells lines	1017:1049	After detailed physicochemical and morphological characterizations of CZTSe QDs and composite membranes, antibacterial activities and cell viability were extensively investigated against gram-positive and gram-negative bacterial and yeast strains, and L929 mouse fibroblast cells lines, respectively.					
36137521	4	73	theme	nanoparticles	706:718	arg1	concentrations					672:685	different concentrations	662:685	different concentrations of the synthesized nanoparticles	662:718	Polymer-based nanocomposites with different concentrations of the synthesized nanoparticles were produced by the solvent casting method.					
36639234	7	0	theme	Laplace	1341:1347	arg1	approximations					1349:1362	Laplace approximations	1341:1362	Laplace approximations to the conditional posterior of the spline vector	1341:1412	Laplace approximations to the conditional posterior of the spline vector are obtained from analytical versions of the gradient and Hessian of the log-likelihood, implying a drastic speed-up in the computation of posterior estimates.					
36639234	9	1	with	epidemics	1743:1751	arg1	combinations					1768:1779	different combinations	1758:1779	different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays)	1758:1866	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	9	2	theme	delay	2024:2028	arg1	patterns					2030:2037	delay patterns	2024:2037	delay patterns	2024:2037	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	7	3	theme	estimates	1563:1571	arg1	computation					1538:1548	the computation	1534:1548	the computation of posterior estimates	1534:1571	Laplace approximations to the conditional posterior of the spline vector are obtained from analytical versions of the gradient and Hessian of the log-likelihood, implying a drastic speed-up in the computation of posterior estimates.					
36639234	9	4	theme	delay	1813:1817	arg1	structure					1819:1827	delay structure	1813:1827	delay structure	1813:1827	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	7	5	theme	drastic	1514:1520	arg1	speed-up					1522:1529	a drastic speed-up	1512:1529	a drastic speed-up	1512:1529	Laplace approximations to the conditional posterior of the spline vector are obtained from analytical versions of the gradient and Hessian of the log-likelihood, implying a drastic speed-up in the computation of posterior estimates.					
36639234	4	6	theme	Bayesian	723:730	arg1	approaches					732:741	Existing Bayesian approaches	714:741	Existing Bayesian approaches	714:741	Existing Bayesian approaches typically rely on Markov chain Monte Carlo algorithms that are extremely costly from a computational perspective.					
36639234	10	7	theme	real	2075:2078	arg1	studies					2085:2091	two real case studies	2071:2091	two real case studies of epidemic outbreaks	2071:2113	Finally, LPS is illustrated in two real case studies of epidemic outbreaks.					
36639234	3	8	theme	misreporting	583:594	arg1	process					596:602	a misreporting process	581:602	a misreporting process	581:602	The main statistical challenge in models that intrinsically account for a misreporting process lies in the joint estimation of the time-varying reproduction number and the delay/underreporting parameters.					
36639234	7	9	theme	log-likelihood	1487:1500	arg1	Hessian					1472:1478	Hessian	1472:1478	Hessian of the log-likelihood	1472:1500	Laplace approximations to the conditional posterior of the spline vector are obtained from analytical versions of the gradient and Hessian of the log-likelihood, implying a drastic speed-up in the computation of posterior estimates.					
36639234	7	9	theme	log-likelihood	1487:1500	arg1	versions					1443:1450	analytical versions	1432:1450	analytical versions of the gradient	1432:1466	Laplace approximations to the conditional posterior of the spline vector are obtained from analytical versions of the gradient and Hessian of the log-likelihood, implying a drastic speed-up in the computation of posterior estimates.					
36639234	6	10	theme	interval	1161:1168	arg1	distribution					1170:1181	a known generation interval distribution	1142:1181	a known generation interval distribution	1142:1181	Assuming a known generation interval distribution, the incidence at a given calendar time is governed by the epidemic renewal equation and the delay structure is specified through a composite link framework.					
36639234	1	11	theme	transmission	215:226	arg1	dynamics					228:235	the transmission dynamics	211:235	the transmission dynamics of an infectious disease	211:260	In epidemic models, the effective reproduction number is of central importance to assess the transmission dynamics of an infectious disease and to orient health intervention strategies.					
36639234	6	12	theme	link	1325:1328	arg1	framework					1330:1338	a composite link framework	1313:1338	a composite link framework	1313:1338	Assuming a known generation interval distribution, the incidence at a given calendar time is governed by the epidemic renewal equation and the delay structure is specified through a composite link framework.					
36639234	10	13	theme	epidemic outbreaks	2096:2113	arg1	studies					2085:2091	two real case studies	2071:2091	two real case studies of epidemic outbreaks	2071:2113	Finally, LPS is illustrated in two real case studies of epidemic outbreaks.					
36639234	1	14	theme	epidemic	125:132	arg1	models					134:139	epidemic models	125:139	epidemic models	125:139	In epidemic models, the effective reproduction number is of central importance to assess the transmission dynamics of an infectious disease and to orient health intervention strategies.					
36639234	9	15	theme	weekend	1852:1858	arg1	delays					1860:1865	weekend delays	1852:1865	weekend delays	1852:1865	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	3	16	theme	reproduction	653:664	arg1	number					666:671	the time-varying reproduction number	636:671	the time-varying reproduction number	636:671	The main statistical challenge in models that intrinsically account for a misreporting process lies in the joint estimation of the time-varying reproduction number and the delay/underreporting parameters.					
36639234	8	17	theme	proposed	1591:1598	arg1	approach					1604:1611	the proposed LPS approach	1587:1611	the proposed LPS approach	1587:1611	Furthermore, the proposed LPS approach can be used to obtain point estimates and approximate credible intervals for the delay and reporting probabilities.					
36639234	0	18	theme	instantaneous	55:67	arg1	number					82:87	the instantaneous reproduction number	51:87	the instantaneous reproduction number	51:87	An approximate Bayesian approach for estimation of the instantaneous reproduction number under misreported epidemic data.					
36639234	5	19	theme	penalized	952:960	arg1	B-splines					962:970	Bayesian penalized B-splines	943:970	Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation	943:1130	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	0	20	theme	number	82:87	arg1	estimation					37:46	estimation	37:46	estimation of the instantaneous reproduction number under misreported epidemic data	37:119	An approximate Bayesian approach for estimation of the instantaneous reproduction number under misreported epidemic data.					
36639234	9	21	theme	proposed	1882:1889	arg1	methodology					1895:1905	the proposed LPS methodology	1878:1905	the proposed LPS methodology	1878:1905	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	0	22	theme	misreported	95:105	arg1	data					116:119	misreported epidemic data	95:119	misreported epidemic data	95:119	An approximate Bayesian approach for estimation of the instantaneous reproduction number under misreported epidemic data.					
36639234	4	23	theme	computational	830:842	arg1	perspective					844:854	a computational perspective	828:854	a computational perspective	828:854	Existing Bayesian approaches typically rely on Markov chain Monte Carlo algorithms that are extremely costly from a computational perspective.					
36639234	5	24	theme	approximations	1060:1073	arg1	estimation					996:1005	flexible and smooth estimation	976:1005	flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation	976:1130	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	9	25	theme	fast	1916:1919	arg1	estimates					1934:1942	fast and accurate estimates	1916:1942	fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns	1916:2037	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	8	26	theme	point	1635:1639	arg1	estimates					1641:1649	point estimates	1635:1649	point estimates	1635:1649	Furthermore, the proposed LPS approach can be used to obtain point estimates and approximate credible intervals for the delay and reporting probabilities.					
36639234	1	27	theme	health	276:281	arg1	strategies					296:305	health intervention strategies	276:305	health intervention strategies	276:305	In epidemic models, the effective reproduction number is of central importance to assess the transmission dynamics of an infectious disease and to orient health intervention strategies.					
36639234	9	28	dep	account	1997:2003	arg1	underreporting					2005:2018	underreporting	2005:2018	underreporting	2005:2018	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	7	29	theme	analytical	1432:1441	arg1	versions					1443:1450	analytical versions	1432:1450	analytical versions of the gradient	1432:1466	Laplace approximations to the conditional posterior of the spline vector are obtained from analytical versions of the gradient and Hessian of the log-likelihood, implying a drastic speed-up in the computation of posterior estimates.					
36639234	9	30	theme	accurate	1925:1932	arg1	estimates					1934:1942	fast and accurate estimates	1916:1942	fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns	1916:2037	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	0	31	theme	Bayesian	15:22	arg1	approach					24:31	An approximate Bayesian approach	0:31	An approximate Bayesian approach for estimation of the instantaneous reproduction number under misreported epidemic data	0:119	An approximate Bayesian approach for estimation of the instantaneous reproduction number under misreported epidemic data.					
36639234	5	32	theme	reproduction	1028:1039	arg1	number					1041:1046	the instantaneous reproduction number	1010:1046	the instantaneous reproduction number	1010:1046	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	6	33	theme	known	1144:1148	arg1	distribution					1170:1181	a known generation interval distribution	1142:1181	a known generation interval distribution	1142:1181	Assuming a known generation interval distribution, the incidence at a given calendar time is governed by the epidemic renewal equation and the delay structure is specified through a composite link framework.					
36639234	2	34	theme	epidemiological	481:495	arg1	parameters					497:506	epidemiological parameters	481:506	epidemiological parameters	481:506	Publicly shared data during an outbreak often suffers from two sources of misreporting (underreporting and delay in reporting) that should not be overlooked when estimating epidemiological parameters.					
36639234	8	35	theme	credible	1667:1674	arg1	intervals					1676:1684	approximate credible intervals	1655:1684	approximate credible intervals	1655:1684	Furthermore, the proposed LPS approach can be used to obtain point estimates and approximate credible intervals for the delay and reporting probabilities.					
36639234	3	36	theme	delay/underreporting	681:700	arg1	parameters					702:711	the delay/underreporting parameters	677:711	the delay/underreporting parameters	677:711	The main statistical challenge in models that intrinsically account for a misreporting process lies in the joint estimation of the time-varying reproduction number and the delay/underreporting parameters.					
36639234	5	37	theme	fast	1115:1118	arg1	computation					1120:1130	fast computation	1115:1130	fast computation	1115:1130	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	7	38	theme	vector	1407:1412	arg1	posterior					1383:1391	the conditional posterior	1367:1391	the conditional posterior of the spline vector	1367:1412	Laplace approximations to the conditional posterior of the spline vector are obtained from analytical versions of the gradient and Hessian of the log-likelihood, implying a drastic speed-up in the computation of posterior estimates.					
36639234	5	39	theme	selected	1078:1085	arg1	distributions					1097:1109	selected posterior distributions	1078:1109	selected posterior distributions for fast computation	1078:1130	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	6	40	theme	calendar	1209:1216	arg1	time					1218:1221	a given calendar time	1201:1221	a given calendar time	1201:1221	Assuming a known generation interval distribution, the incidence at a given calendar time is governed by the epidemic renewal equation and the delay structure is specified through a composite link framework.					
36639234	9	41	theme	different	1758:1766	arg1	combinations					1768:1779	different combinations	1758:1779	different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays)	1758:1866	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	3	42	theme	statistical	518:528	arg1	challenge					530:538	The main statistical challenge	509:538	The main statistical challenge in models that intrinsically account for a misreporting process	509:602	The main statistical challenge in models that intrinsically account for a misreporting process lies in the joint estimation of the time-varying reproduction number and the delay/underreporting parameters.					
36639234	1	43	theme	disease	254:260	arg1	dynamics					228:235	the transmission dynamics	211:235	the transmission dynamics of an infectious disease	211:260	In epidemic models, the effective reproduction number is of central importance to assess the transmission dynamics of an infectious disease and to orient health intervention strategies.					
36639234	3	44	from	challenge	530:538	arg1	models					543:548	models	543:548	models that intrinsically account for a misreporting process	543:602	The main statistical challenge in models that intrinsically account for a misreporting process lies in the joint estimation of the time-varying reproduction number and the delay/underreporting parameters.					
36639234	6	45	theme	renewal	1251:1257	arg1	equation and					1259:1270	the epidemic renewal equation and	1238:1270	the epidemic renewal equation and	1238:1270	Assuming a known generation interval distribution, the incidence at a given calendar time is governed by the epidemic renewal equation and the delay structure is specified through a composite link framework.					
36639234	9	46	theme	underreporting	1789:1802	arg1	rate					1804:1807	underreporting rate	1789:1807	underreporting rate	1789:1807	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	6	47	theme	delay	1276:1280	arg1	structure					1282:1290	the delay structure	1272:1290	the delay structure	1272:1290	Assuming a known generation interval distribution, the incidence at a given calendar time is governed by the epidemic renewal equation and the delay structure is specified through a composite link framework.					
36639234	7	48	theme	posterior	1553:1561	arg1	estimates					1563:1571	posterior estimates	1553:1571	posterior estimates	1553:1571	Laplace approximations to the conditional posterior of the spline vector are obtained from analytical versions of the gradient and Hessian of the log-likelihood, implying a drastic speed-up in the computation of posterior estimates.					
36639234	1	49	theme	central	182:188	arg1	importance					190:199	central importance	182:199	central importance	182:199	In epidemic models, the effective reproduction number is of central importance to assess the transmission dynamics of an infectious disease and to orient health intervention strategies.					
36639234	6	50	theme	epidemic	1242:1249	arg1	equation and					1259:1270	the epidemic renewal equation and	1238:1270	the epidemic renewal equation and	1238:1270	Assuming a known generation interval distribution, the incidence at a given calendar time is governed by the epidemic renewal equation and the delay structure is specified through a composite link framework.					
36639234	4	51	theme	Existing	714:721	arg1	approaches					732:741	Existing Bayesian approaches	714:741	Existing Bayesian approaches	714:741	Existing Bayesian approaches typically rely on Markov chain Monte Carlo algorithms that are extremely costly from a computational perspective.					
36639234	10	52	theme	case	2080:2083	arg1	studies					2085:2091	two real case studies	2071:2091	two real case studies of epidemic outbreaks	2071:2113	Finally, LPS is illustrated in two real case studies of epidemic outbreaks.					
36639234	2	53	theme	shared	317:322	arg1	data					324:327	Publicly shared data	308:327	Publicly shared data during an outbreak	308:346	Publicly shared data during an outbreak often suffers from two sources of misreporting (underreporting and delay in reporting) that should not be overlooked when estimating epidemiological parameters.					
36639234	3	54	theme	joint	616:620	arg1	estimation					622:631	the joint estimation	612:631	the joint estimation of the time-varying reproduction number and the delay/underreporting parameters	612:711	The main statistical challenge in models that intrinsically account for a misreporting process lies in the joint estimation of the time-varying reproduction number and the delay/underreporting parameters.					
36639234	5	55	theme	flexible	976:983	arg1	estimation					996:1005	flexible and smooth estimation	976:1005	flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation	976:1130	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	6	56	theme	composite	1315:1323	arg1	framework					1330:1338	a composite link framework	1313:1338	a composite link framework	1313:1338	Assuming a known generation interval distribution, the incidence at a given calendar time is governed by the epidemic renewal equation and the delay structure is specified through a composite link framework.					
36639234	3	57	theme	time-varying	640:651	arg1	number					666:671	the time-varying reproduction number	636:671	the time-varying reproduction number	636:671	The main statistical challenge in models that intrinsically account for a misreporting process lies in the joint estimation of the time-varying reproduction number and the delay/underreporting parameters.					
36639234	5	58	theme	smooth	989:994	arg1	estimation					996:1005	flexible and smooth estimation	976:1005	flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation	976:1130	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	8	59	theme	LPS	1600:1602	arg1	approach					1604:1611	the proposed LPS approach	1587:1611	the proposed LPS approach	1587:1611	Furthermore, the proposed LPS approach can be used to obtain point estimates and approximate credible intervals for the delay and reporting probabilities.					
36639234	8	60	used	used	1620:1623	arg2	approach					1604:1611	the proposed LPS approach	1587:1611	the proposed LPS approach	1587:1611	Furthermore, the proposed LPS approach can be used to obtain point estimates and approximate credible intervals for the delay and reporting probabilities.					
36639234	0	61	theme	reproduction	69:80	arg1	number					82:87	the instantaneous reproduction number	51:87	the instantaneous reproduction number	51:87	An approximate Bayesian approach for estimation of the instantaneous reproduction number under misreported epidemic data.					
36639234	1	62	from	importance	190:199	arg1	models					134:139	epidemic models	125:139	epidemic models	125:139	In epidemic models, the effective reproduction number is of central importance to assess the transmission dynamics of an infectious disease and to orient health intervention strategies.					
36639234	5	63	theme	Bayesian	943:950	arg1	B-splines					962:970	Bayesian penalized B-splines	943:970	Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation	943:1130	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	7	64	theme	gradient	1459:1466	arg1	Hessian					1472:1478	Hessian	1472:1478	Hessian of the log-likelihood	1472:1500	Laplace approximations to the conditional posterior of the spline vector are obtained from analytical versions of the gradient and Hessian of the log-likelihood, implying a drastic speed-up in the computation of posterior estimates.					
36639234	7	64	theme	gradient	1459:1466	arg1	versions					1443:1450	analytical versions	1432:1450	analytical versions of the gradient	1432:1466	Laplace approximations to the conditional posterior of the spline vector are obtained from analytical versions of the gradient and Hessian of the log-likelihood, implying a drastic speed-up in the computation of posterior estimates.					
36639234	4	65	from	perspective	844:854	arg1	costly					816:821	costly	816:821	costly	816:821	Existing Bayesian approaches typically rely on Markov chain Monte Carlo algorithms that are extremely costly from a computational perspective.					
36639234	1	66	theme	reproduction	156:167	arg1	number					169:174	the effective reproduction number	142:174	the effective reproduction number	142:174	In epidemic models, the effective reproduction number is of central importance to assess the transmission dynamics of an infectious disease and to orient health intervention strategies.					
36639234	0	67	theme	epidemic	107:114	arg1	data					116:119	misreported epidemic data	95:119	misreported epidemic data	95:119	An approximate Bayesian approach for estimation of the instantaneous reproduction number under misreported epidemic data.					
36639234	9	68	theme	epidemics	1743:1751	arg1	Simulation					1729:1738	Simulation	1729:1738	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays)	1729:1866	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	5	69	theme	number	1041:1046	arg1	estimation					996:1005	flexible and smooth estimation	976:1005	flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation	976:1130	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	9	70	theme	LPS	1891:1893	arg1	methodology					1895:1905	the proposed LPS methodology	1878:1905	the proposed LPS methodology	1878:1905	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	5	71	theme	Laplace	1052:1058	arg1	approximations					1060:1073	Laplace approximations	1052:1073	Laplace approximations	1052:1073	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	0	72	theme	approximate	3:13	arg1	approach					24:31	An approximate Bayesian approach	0:31	An approximate Bayesian approach for estimation of the instantaneous reproduction number under misreported epidemic data	0:119	An approximate Bayesian approach for estimation of the instantaneous reproduction number under misreported epidemic data.					
36639234	8	73	theme	approximate	1655:1665	arg1	intervals					1676:1684	approximate credible intervals	1655:1684	approximate credible intervals	1655:1684	Furthermore, the proposed LPS approach can be used to obtain point estimates and approximate credible intervals for the delay and reporting probabilities.					
36639234	2	74	theme	misreporting	382:393	arg1	sources					371:377	two sources	367:377	two sources of misreporting (underreporting and delay in reporting) that should not be overlooked when estimating epidemiological parameters	367:506	Publicly shared data during an outbreak often suffers from two sources of misreporting (underreporting and delay in reporting) that should not be overlooked when estimating epidemiological parameters.					
36639234	5	75	theme	faster	875:880	arg1	alternative					882:892	a much faster alternative	868:892	a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation	868:1130	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	1	76	theme	intervention	283:294	arg1	strategies					296:305	health intervention strategies	276:305	health intervention strategies	276:305	In epidemic models, the effective reproduction number is of central importance to assess the transmission dynamics of an infectious disease and to orient health intervention strategies.					
36639234	5	77	theme	instantaneous	1014:1026	arg1	number					1041:1046	the instantaneous reproduction number	1010:1046	the instantaneous reproduction number	1010:1046	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	6	78	theme	generation	1150:1159	arg1	distribution					1170:1181	a known generation interval distribution	1142:1181	a known generation interval distribution	1142:1181	Assuming a known generation interval distribution, the incidence at a given calendar time is governed by the epidemic renewal equation and the delay structure is specified through a composite link framework.					
36639234	7	79	theme	spline	1400:1405	arg1	vector					1407:1412	the spline vector	1396:1412	the spline vector	1396:1412	Laplace approximations to the conditional posterior of the spline vector are obtained from analytical versions of the gradient and Hessian of the log-likelihood, implying a drastic speed-up in the computation of posterior estimates.					
36639234	9	80	theme	existing	1958:1965	arg1	methods					1967:1973	existing methods	1958:1973	existing methods that do not take into account underreporting and delay patterns	1958:2037	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	3	81	theme	number	666:671	arg1	estimation					622:631	the joint estimation	612:631	the joint estimation of the time-varying reproduction number and the delay/underreporting parameters	612:711	The main statistical challenge in models that intrinsically account for a misreporting process lies in the joint estimation of the time-varying reproduction number and the delay/underreporting parameters.					
36639234	6	82	from	time	1218:1221	arg1	incidence					1188:1196	the incidence	1184:1196	the incidence at a given calendar time	1184:1221	Assuming a known generation interval distribution, the incidence at a given calendar time is governed by the epidemic renewal equation and the delay structure is specified through a composite link framework.					
36639234	9	83	dep	rate	1804:1807	arg1	the					1785:1787	the	1785:1787	the	1785:1787	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	9	83	dep	rate	1804:1807	arg1	two-day					1839:1845	two-day	1839:1845	two-day	1839:1845	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	9	83	dep	rate	1804:1807	arg1	one-day					1830:1836	one-day	1830:1836	one-day	1830:1836	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	9	83	dep	rate	1804:1807	arg1	delays					1860:1865	weekend delays	1852:1865	weekend delays	1852:1865	Simulation of epidemics with different combinations for the underreporting rate and delay structure (one-day, two-day, and weekend delays) show that the proposed LPS methodology delivers fast and accurate estimates outperforming existing methods that do not take into account underreporting and delay patterns.					
36639234	1	84	theme	effective	146:154	arg1	number					169:174	the effective reproduction number	142:174	the effective reproduction number	142:174	In epidemic models, the effective reproduction number is of central importance to assess the transmission dynamics of an infectious disease and to orient health intervention strategies.					
36639234	3	85	theme	main	513:516	arg1	challenge					530:538	The main statistical challenge	509:538	The main statistical challenge in models that intrinsically account for a misreporting process	509:602	The main statistical challenge in models that intrinsically account for a misreporting process lies in the joint estimation of the time-varying reproduction number and the delay/underreporting parameters.					
36639234	2	86	from	underreporting	396:409	arg1	reporting					424:432	reporting	424:432	reporting	424:432	Publicly shared data during an outbreak often suffers from two sources of misreporting (underreporting and delay in reporting) that should not be overlooked when estimating epidemiological parameters.					
36639234	7	87	theme	conditional	1371:1381	arg1	posterior					1383:1391	the conditional posterior	1367:1391	the conditional posterior of the spline vector	1367:1412	Laplace approximations to the conditional posterior of the spline vector are obtained from analytical versions of the gradient and Hessian of the log-likelihood, implying a drastic speed-up in the computation of posterior estimates.					
36639234	3	88	theme	parameters	702:711	arg1	estimation					622:631	the joint estimation	612:631	the joint estimation of the time-varying reproduction number and the delay/underreporting parameters	612:711	The main statistical challenge in models that intrinsically account for a misreporting process lies in the joint estimation of the time-varying reproduction number and the delay/underreporting parameters.					
36639234	2	89	dep	misreporting	382:393	arg1	underreporting					396:409	underreporting	396:409	underreporting	396:409	Publicly shared data during an outbreak often suffers from two sources of misreporting (underreporting and delay in reporting) that should not be overlooked when estimating epidemiological parameters.					
36639234	2	89	dep	misreporting	382:393	arg1	delay					415:419	delay	415:419	delay	415:419	Publicly shared data during an outbreak often suffers from two sources of misreporting (underreporting and delay in reporting) that should not be overlooked when estimating epidemiological parameters.					
36639234	5	90	theme	posterior	1087:1095	arg1	distributions					1097:1109	selected posterior distributions	1078:1109	selected posterior distributions for fast computation	1078:1130	We propose a much faster alternative based on Laplacian-P-splines (LPS) that combines Bayesian penalized B-splines for flexible and smooth estimation of the instantaneous reproduction number and Laplace approximations to selected posterior distributions for fast computation.					
36639234	2	91	from	delay	415:419	arg1	reporting					424:432	reporting	424:432	reporting	424:432	Publicly shared data during an outbreak often suffers from two sources of misreporting (underreporting and delay in reporting) that should not be overlooked when estimating epidemiological parameters.					
36639234	6	92	theme	given	1203:1207	arg1	time					1218:1221	a given calendar time	1201:1221	a given calendar time	1201:1221	Assuming a known generation interval distribution, the incidence at a given calendar time is governed by the epidemic renewal equation and the delay structure is specified through a composite link framework.					
36639234	1	93	theme	infectious	243:252	arg1	disease					254:260	an infectious disease	240:260	an infectious disease	240:260	In epidemic models, the effective reproduction number is of central importance to assess the transmission dynamics of an infectious disease and to orient health intervention strategies.					
36077353	7	0	from	increase	724:731	arg1	density					757:763	crosslinking network density	736:763	crosslinking network density	736:763	The results indicate that, with an increase in crosslinking network density, the stiffness of hydrogels became higher; these hydrogels have more of a compact pore structure, their anti-degradation property is improved, and swelling property is reduced.					
36077353	7	1	theme	network	749:755	arg1	density					757:763	crosslinking network density	736:763	crosslinking network density	736:763	The results indicate that, with an increase in crosslinking network density, the stiffness of hydrogels became higher; these hydrogels have more of a compact pore structure, their anti-degradation property is improved, and swelling property is reduced.					
36077353	8	2	from	change	1050:1055	arg1	stability					1064:1072	stability	1064:1072	stability	1064:1072	The introduction of hydroxyapatite greatly improved the mechanical properties of hydrogels, but there is no change in the stability and crosslinking network structure of hydrogels.					
36077353	8	2	from	change	1050:1055	arg1	structure					1099:1107	crosslinking network structure	1078:1107	crosslinking network structure	1078:1107	The introduction of hydroxyapatite greatly improved the mechanical properties of hydrogels, but there is no change in the stability and crosslinking network structure of hydrogels.					
36077353	5	3	theme	double-crosslinked	461:478	arg1	hydrogel					480:487	the double-crosslinked hydrogel	457:487	the double-crosslinked hydrogel (HG)	457:492	Hence, the double-crosslinked hydrogel (HG) was formed by the photo-crosslinking of double bonds and the oxidative-crosslinking of catechins.					
36077353	5	3	theme	double-crosslinked	461:478	arg1	HG					490:491	HG	490:491	HG	490:491	Hence, the double-crosslinked hydrogel (HG) was formed by the photo-crosslinking of double bonds and the oxidative-crosslinking of catechins.					
36077353	5	4	link	double-crosslinked	461:478	arg1	hydrogel					480:487	the double-crosslinked hydrogel	457:487	the double-crosslinked hydrogel (HG)	457:492	Hence, the double-crosslinked hydrogel (HG) was formed by the photo-crosslinking of double bonds and the oxidative-crosslinking of catechins.					
36077353	5	4	link	double-crosslinked	461:478	arg1	HG					490:491	HG	490:491	HG	490:491	Hence, the double-crosslinked hydrogel (HG) was formed by the photo-crosslinking of double bonds and the oxidative-crosslinking of catechins.					
36077353	7	5	theme	swelling	912:919	arg1	property					921:928	swelling property	912:928	swelling property	912:928	The results indicate that, with an increase in crosslinking network density, the stiffness of hydrogels became higher; these hydrogels have more of a compact pore structure, their anti-degradation property is improved, and swelling property is reduced.					
36077353	7	6	theme	hydrogels	783:791	arg1	stiffness					770:778	the stiffness	766:778	the stiffness of hydrogels	766:791	The results indicate that, with an increase in crosslinking network density, the stiffness of hydrogels became higher; these hydrogels have more of a compact pore structure, their anti-degradation property is improved, and swelling property is reduced.					
36077353	8	7	theme	network	1091:1097	arg1	structure					1099:1107	crosslinking network structure	1078:1107	crosslinking network structure	1078:1107	The introduction of hydroxyapatite greatly improved the mechanical properties of hydrogels, but there is no change in the stability and crosslinking network structure of hydrogels.					
36077353	4	8	theme	mussels	441:447	arg1	chemistry					421:429	the adhesion chemistry	408:429	the adhesion chemistry of marine mussels	408:447	The catechin group was further introduced into the HA chain inspired by the adhesion chemistry of marine mussels.					
36077353	7	9	contain	have	824:827	arg1	hydrogels					814:822	these hydrogels	808:822	these hydrogels	808:822	The results indicate that, with an increase in crosslinking network density, the stiffness of hydrogels became higher; these hydrogels have more of a compact pore structure, their anti-degradation property is improved, and swelling property is reduced.					
36077353	7	9	contain	have	824:827	arg2	more					829:832	more	829:832	more	829:832	The results indicate that, with an increase in crosslinking network density, the stiffness of hydrogels became higher; these hydrogels have more of a compact pore structure, their anti-degradation property is improved, and swelling property is reduced.					
36077353	2	10	theme	photo-oxidized	209:222	arg1	hydrogels					243:251	hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels	185:251	hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels	185:251	This study designed hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels.					
36077353	8	11	theme	crosslinking	1078:1089	arg1	structure					1099:1107	crosslinking network structure	1078:1107	crosslinking network structure	1078:1107	The introduction of hydroxyapatite greatly improved the mechanical properties of hydrogels, but there is no change in the stability and crosslinking network structure of hydrogels.					
36077353	1	12	theme	Natural	85:91	arg1	hydrogels					101:109	Natural polymer hydrogels	85:109	Natural polymer hydrogels	85:109	Natural polymer hydrogels have good mechanical properties and biocompatibility.					
36077353	5	13	theme	double	534:539	arg1	bonds					541:545	double bonds	534:545	double bonds	534:545	Hence, the double-crosslinked hydrogel (HG) was formed by the photo-crosslinking of double bonds and the oxidative-crosslinking of catechins.					
36077353	2	14	theme	hydroxyapatite-enhanced	185:207	arg1	hydrogels					243:251	hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels	185:251	hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels	185:251	This study designed hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels.					
36077353	9	15	theme	inorganic	1129:1137	arg1	hydrogels					1154:1162	These inorganic phase-enhanced hydrogels	1123:1162	These inorganic phase-enhanced hydrogels	1123:1162	These inorganic phase-enhanced hydrogels were expected to be applied to tissue engineering scaffolds.					
36077353	1	16	theme	polymer	93:99	arg1	hydrogels					101:109	Natural polymer hydrogels	85:109	Natural polymer hydrogels	85:109	Natural polymer hydrogels have good mechanical properties and biocompatibility.					
36077353	4	17	theme	catechin	340:347	arg1	group					349:353	The catechin group	336:353	The catechin group	336:353	The catechin group was further introduced into the HA chain inspired by the adhesion chemistry of marine mussels.					
36077353	8	18	dep	stability	1064:1072	arg1	the					1060:1062	the	1060:1062	the	1060:1062	The introduction of hydroxyapatite greatly improved the mechanical properties of hydrogels, but there is no change in the stability and crosslinking network structure of hydrogels.					
36077353	9	19	theme	phase-enhanced	1139:1152	arg1	hydrogels					1154:1162	These inorganic phase-enhanced hydrogels	1123:1162	These inorganic phase-enhanced hydrogels	1123:1162	These inorganic phase-enhanced hydrogels were expected to be applied to tissue engineering scaffolds.					
36077353	2	20	link	double-crosslinked	224:241	arg1	hydrogels					243:251	hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels	185:251	hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels	185:251	This study designed hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels.					
36077353	7	21	theme	compact	839:845	arg1	structure					852:860	a compact pore structure	837:860	a compact pore structure	837:860	The results indicate that, with an increase in crosslinking network density, the stiffness of hydrogels became higher; these hydrogels have more of a compact pore structure, their anti-degradation property is improved, and swelling property is reduced.					
36077353	4	22	theme	adhesion	412:419	arg1	chemistry					421:429	the adhesion chemistry	408:429	the adhesion chemistry of marine mussels	408:447	The catechin group was further introduced into the HA chain inspired by the adhesion chemistry of marine mussels.					
36077353	7	23	theme	pore	847:850	arg1	structure					852:860	a compact pore structure	837:860	a compact pore structure	837:860	The results indicate that, with an increase in crosslinking network density, the stiffness of hydrogels became higher; these hydrogels have more of a compact pore structure, their anti-degradation property is improved, and swelling property is reduced.					
36077353	8	24	theme	hydrogels	1112:1120	arg1	stability					1064:1072	stability	1064:1072	stability	1064:1072	The introduction of hydroxyapatite greatly improved the mechanical properties of hydrogels, but there is no change in the stability and crosslinking network structure of hydrogels.					
36077353	8	24	theme	hydrogels	1112:1120	arg1	structure					1099:1107	crosslinking network structure	1078:1107	crosslinking network structure	1078:1107	The introduction of hydroxyapatite greatly improved the mechanical properties of hydrogels, but there is no change in the stability and crosslinking network structure of hydrogels.					
36077353	4	25	theme	marine	434:439	arg1	mussels					441:447	marine mussels	434:447	marine mussels	434:447	The catechin group was further introduced into the HA chain inspired by the adhesion chemistry of marine mussels.					
36077353	1	26	theme	good	116:119	arg1	properties					132:141	good mechanical properties	116:141	good mechanical properties	116:141	Natural polymer hydrogels have good mechanical properties and biocompatibility.					
36077353	7	27	theme	anti-degradation	869:884	arg1	property					886:893	their anti-degradation property	863:893	their anti-degradation property	863:893	The results indicate that, with an increase in crosslinking network density, the stiffness of hydrogels became higher; these hydrogels have more of a compact pore structure, their anti-degradation property is improved, and swelling property is reduced.					
36077353	6	28	theme	hydroxyapatite-enhanced	648:670	arg1	HGH					683:685	HGH	683:685	HGH	683:685	Moreover, hydroxyapatite was introduced into HG to form hydroxyapatite-enhanced hydrogels (HGH).					
36077353	6	28	theme	hydroxyapatite-enhanced	648:670	arg1	hydrogels					672:680	hydroxyapatite-enhanced hydrogels	648:680	hydroxyapatite-enhanced hydrogels (HGH)	648:686	Moreover, hydroxyapatite was introduced into HG to form hydroxyapatite-enhanced hydrogels (HGH).					
36077353	0	29	link	Double-Crosslinked	30:47	arg1	Hydrogels					74:82	Double-Crosslinked Hydroxyapatite Composite Hydrogels	30:82	Double-Crosslinked Hydroxyapatite Composite Hydrogels	30:82	Preparation and Properties of Double-Crosslinked Hydroxyapatite Composite Hydrogels.					
36077353	1	30	theme	mechanical	121:130	arg1	properties					132:141	good mechanical properties	116:141	good mechanical properties	116:141	Natural polymer hydrogels have good mechanical properties and biocompatibility.					
36077353	0	31	theme	Hydroxyapatite	49:62	arg1	Hydrogels					74:82	Double-Crosslinked Hydroxyapatite Composite Hydrogels	30:82	Double-Crosslinked Hydroxyapatite Composite Hydrogels	30:82	Preparation and Properties of Double-Crosslinked Hydroxyapatite Composite Hydrogels.					
36077353	5	32	theme	bonds	541:545	arg1	photo-crosslinking					512:529	the photo-crosslinking	508:529	the photo-crosslinking of double bonds	508:545	Hence, the double-crosslinked hydrogel (HG) was formed by the photo-crosslinking of double bonds and the oxidative-crosslinking of catechins.					
36077353	5	32	theme	bonds	541:545	arg1	oxidative-crosslinking					555:576	the oxidative-crosslinking	551:576	the oxidative-crosslinking of catechins	551:589	Hence, the double-crosslinked hydrogel (HG) was formed by the photo-crosslinking of double bonds and the oxidative-crosslinking of catechins.					
36077353	3	33	theme	methacrylate	312:323	arg1	anhydride					325:333	methacrylate anhydride	312:333	methacrylate anhydride	312:333	Hyaluronic acid (HA) and gelatin (Gel) were modified with methacrylate anhydride.					
36077353	0	34	theme	Double-Crosslinked	30:47	arg1	Hydrogels					74:82	Double-Crosslinked Hydroxyapatite Composite Hydrogels	30:82	Double-Crosslinked Hydroxyapatite Composite Hydrogels	30:82	Preparation and Properties of Double-Crosslinked Hydroxyapatite Composite Hydrogels.					
36077353	3	35	mod	modified	298:305	arg1	Gel					288:290	Gel	288:290	Gel	288:290	Hyaluronic acid (HA) and gelatin (Gel) were modified with methacrylate anhydride.					
36077353	3	35	mod	modified	298:305	arg3	anhydride					325:333	methacrylate anhydride	312:333	methacrylate anhydride	312:333	Hyaluronic acid (HA) and gelatin (Gel) were modified with methacrylate anhydride.					
36077353	3	35	mod	modified	298:305	arg1	HA					271:272	HA	271:272	HA	271:272	Hyaluronic acid (HA) and gelatin (Gel) were modified with methacrylate anhydride.					
36077353	3	35	mod	modified	298:305	arg1	gelatin					279:285	gelatin	279:285	gelatin (Gel)	279:291	Hyaluronic acid (HA) and gelatin (Gel) were modified with methacrylate anhydride.					
36077353	3	35	mod	modified	298:305	arg1	acid					265:268	Hyaluronic acid	254:268	Hyaluronic acid (HA)	254:273	Hyaluronic acid (HA) and gelatin (Gel) were modified with methacrylate anhydride.					
36077353	7	36	theme	crosslinking	736:747	arg1	density					757:763	crosslinking network density	736:763	crosslinking network density	736:763	The results indicate that, with an increase in crosslinking network density, the stiffness of hydrogels became higher; these hydrogels have more of a compact pore structure, their anti-degradation property is improved, and swelling property is reduced.					
36077353	0	37	theme	Hydrogels	74:82	arg1	Properties					16:25	Properties	16:25	Properties	16:25	Preparation and Properties of Double-Crosslinked Hydroxyapatite Composite Hydrogels.					
36077353	0	37	theme	Hydrogels	74:82	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Properties of Double-Crosslinked Hydroxyapatite Composite Hydrogels.					
36077353	2	38	theme	double-crosslinked	224:241	arg1	hydrogels					243:251	hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels	185:251	hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels	185:251	This study designed hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels.					
36077353	8	39	theme	hydroxyapatite	962:975	arg1	introduction					946:957	The introduction	942:957	The introduction of hydroxyapatite	942:975	The introduction of hydroxyapatite greatly improved the mechanical properties of hydrogels, but there is no change in the stability and crosslinking network structure of hydrogels.					
36077353	0	40	theme	Composite	64:72	arg1	Hydrogels					74:82	Double-Crosslinked Hydroxyapatite Composite Hydrogels	30:82	Double-Crosslinked Hydroxyapatite Composite Hydrogels	30:82	Preparation and Properties of Double-Crosslinked Hydroxyapatite Composite Hydrogels.					
36077353	9	41	theme	tissue	1195:1200	arg1	scaffolds					1214:1222	tissue engineering scaffolds	1195:1222	tissue engineering scaffolds	1195:1222	These inorganic phase-enhanced hydrogels were expected to be applied to tissue engineering scaffolds.					
36077353	1	42	contain	have	111:114	arg2	properties					132:141	good mechanical properties	116:141	good mechanical properties	116:141	Natural polymer hydrogels have good mechanical properties and biocompatibility.					
36077353	1	42	contain	have	111:114	arg1	hydrogels					101:109	Natural polymer hydrogels	85:109	Natural polymer hydrogels	85:109	Natural polymer hydrogels have good mechanical properties and biocompatibility.					
36077353	1	42	contain	have	111:114	arg2	biocompatibility					147:162	biocompatibility	147:162	biocompatibility	147:162	Natural polymer hydrogels have good mechanical properties and biocompatibility.					
36077353	8	43	theme	hydrogels	1023:1031	arg1	properties					1009:1018	the mechanical properties	994:1018	the mechanical properties of hydrogels	994:1031	The introduction of hydroxyapatite greatly improved the mechanical properties of hydrogels, but there is no change in the stability and crosslinking network structure of hydrogels.					
36077353	3	44	theme	Hyaluronic	254:263	arg1	HA					271:272	HA	271:272	HA	271:272	Hyaluronic acid (HA) and gelatin (Gel) were modified with methacrylate anhydride.					
36077353	3	44	theme	Hyaluronic	254:263	arg1	acid					265:268	Hyaluronic acid	254:268	Hyaluronic acid (HA)	254:273	Hyaluronic acid (HA) and gelatin (Gel) were modified with methacrylate anhydride.					
36077353	5	45	theme	catechins	581:589	arg1	photo-crosslinking					512:529	the photo-crosslinking	508:529	the photo-crosslinking of double bonds	508:545	Hence, the double-crosslinked hydrogel (HG) was formed by the photo-crosslinking of double bonds and the oxidative-crosslinking of catechins.					
36077353	5	45	theme	catechins	581:589	arg1	oxidative-crosslinking					555:576	the oxidative-crosslinking	551:576	the oxidative-crosslinking of catechins	551:589	Hence, the double-crosslinked hydrogel (HG) was formed by the photo-crosslinking of double bonds and the oxidative-crosslinking of catechins.					
36077353	9	46	theme	engineering	1202:1212	arg1	scaffolds					1214:1222	tissue engineering scaffolds	1195:1222	tissue engineering scaffolds	1195:1222	These inorganic phase-enhanced hydrogels were expected to be applied to tissue engineering scaffolds.					
36077353	8	47	theme	mechanical	998:1007	arg1	properties					1009:1018	the mechanical properties	994:1018	the mechanical properties of hydrogels	994:1031	The introduction of hydroxyapatite greatly improved the mechanical properties of hydrogels, but there is no change in the stability and crosslinking network structure of hydrogels.					
36077353	4	48	theme	HA	387:388	arg1	chain					390:394	the HA chain	383:394	the HA chain inspired by the adhesion chemistry of marine mussels	383:447	The catechin group was further introduced into the HA chain inspired by the adhesion chemistry of marine mussels.					
35513104	0	0	theme	polydopamine	119:130	arg1	nanoparticles					132:144	bioinspired polydopamine nanoparticles	107:144	bioinspired polydopamine nanoparticles	107:144	High-performance multifunctional polyvinyl alcohol/starch based active packaging films compatibilized with bioinspired polydopamine nanoparticles.					
35513104	4	1	theme	PVA/ST	815:820	arg1	film					822:825	virgin PVA/ST film	808:825	virgin PVA/ST film	808:825	Compared with virgin PVA/ST film, the resulting PVA/ST/PDA nanocomposite films exhibited greatly improved tensile strength, toughness, thermal stability, and water vapor barrier ability.					
35513104	3	2	theme	compatibilization	768:784	arg1	effect					786:791	their compatibilization effect	762:791	their compatibilization effect	762:791	Results demonstrated that the added PDA nanoparticles were evenly dispersed in the PVA/ST matrix, providing compact and dense nanocomposite films due to their compatibilization effect.					
35513104	1	3	theme	high-performance	307:322	arg1	film					350:353	high-performance multifunctional packaging film	307:353	high-performance multifunctional packaging film	307:353	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	0	4	theme	bioinspired	107:117	arg1	nanoparticles					132:144	bioinspired polydopamine nanoparticles	107:144	bioinspired polydopamine nanoparticles	107:144	High-performance multifunctional polyvinyl alcohol/starch based active packaging films compatibilized with bioinspired polydopamine nanoparticles.					
35513104	3	5	theme	nanocomposite	735:747	arg1	films					749:753	compact and dense nanocomposite films	717:753	compact and dense nanocomposite films	717:753	Results demonstrated that the added PDA nanoparticles were evenly dispersed in the PVA/ST matrix, providing compact and dense nanocomposite films due to their compatibilization effect.					
35513104	2	6	theme	mechanical	414:423	arg1	barrier					447:453	water vapor barrier	435:453	water vapor barrier	435:453	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	1	7	theme	multifunctional	324:338	arg1	film					350:353	high-performance multifunctional packaging film	307:353	high-performance multifunctional packaging film	307:353	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	2	8	theme	composite	569:577	arg1	films					579:583	PVA/ST composite films	562:583	PVA/ST composite films	562:583	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	4	9	theme	water	952:956	arg1	ability					972:978	water vapor barrier ability	952:978	water vapor barrier ability	952:978	Compared with virgin PVA/ST film, the resulting PVA/ST/PDA nanocomposite films exhibited greatly improved tensile strength, toughness, thermal stability, and water vapor barrier ability.					
35513104	5	10	theme	composite	1029:1037	arg1	film					1039:1042	PVA/ST composite film	1022:1042	PVA/ST composite film	1022:1042	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	4	11	theme	virgin	808:813	arg1	film					822:825	virgin PVA/ST film	808:825	virgin PVA/ST film	808:825	Compared with virgin PVA/ST film, the resulting PVA/ST/PDA nanocomposite films exhibited greatly improved tensile strength, toughness, thermal stability, and water vapor barrier ability.					
35513104	1	12	theme	packaging	340:348	arg1	film					350:353	high-performance multifunctional packaging film	307:353	high-performance multifunctional packaging film	307:353	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	2	13	theme	PVA/ST	562:567	arg1	films					579:583	PVA/ST composite films	562:583	PVA/ST composite films	562:583	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	6	14	theme	photothermal	1248:1259	arg1	deterioration					1271:1283	photothermal oxidative deterioration	1248:1283	photothermal oxidative deterioration	1248:1283	Thus, such high-performance multifunctional nanocomposite films hold the potential of protecting food quality against photothermal oxidative deterioration and extend food shelf life.					
35513104	2	15	dep	mechanical	414:423	arg1	thermal					426:432	thermal	426:432	thermal	426:432	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	16	theme	UV	469:470	arg1	light					490:494	ultraviolet (UV)/high-energy blue light	456:494	ultraviolet (UV)/high-energy blue light (HEBL) blocking	456:510	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	16	theme	UV	469:470	arg1	HEBL					497:500	HEBL	497:500	HEBL	497:500	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	4	17	theme	resulting	832:840	arg1	films					867:871	the resulting PVA/ST/PDA nanocomposite films	828:871	the resulting PVA/ST/PDA nanocomposite films	828:871	Compared with virgin PVA/ST film, the resulting PVA/ST/PDA nanocomposite films exhibited greatly improved tensile strength, toughness, thermal stability, and water vapor barrier ability.					
35513104	6	18	theme	such	1136:1139	arg1	films					1188:1192	such high-performance multifunctional nanocomposite films	1136:1192	such high-performance multifunctional nanocomposite films	1136:1192	Thus, such high-performance multifunctional nanocomposite films hold the potential of protecting food quality against photothermal oxidative deterioration and extend food shelf life.					
35513104	6	19	theme	multifunctional	1158:1172	arg1	films					1188:1192	such high-performance multifunctional nanocomposite films	1136:1192	such high-performance multifunctional nanocomposite films	1136:1192	Thus, such high-performance multifunctional nanocomposite films hold the potential of protecting food quality against photothermal oxidative deterioration and extend food shelf life.					
35513104	0	20	theme	High-performance	0:15	arg1	alcohol/starch					43:56	High-performance multifunctional polyvinyl alcohol/starch	0:56	High-performance multifunctional polyvinyl alcohol/starch	0:56	High-performance multifunctional polyvinyl alcohol/starch based active packaging films compatibilized with bioinspired polydopamine nanoparticles.					
35513104	6	21	theme	nanocomposite	1174:1186	arg1	films					1188:1192	such high-performance multifunctional nanocomposite films	1136:1192	such high-performance multifunctional nanocomposite films	1136:1192	Thus, such high-performance multifunctional nanocomposite films hold the potential of protecting food quality against photothermal oxidative deterioration and extend food shelf life.					
35513104	1	22	theme	functional	225:234	arg1	compatibilizers					236:250	functional compatibilizers	225:250	functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix	225:294	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	1	22	theme	functional	225:234	arg1	nanoparticles					178:190	Bioinspired polydopamine (PDA) nanoparticles	147:190	Bioinspired polydopamine (PDA) nanoparticles	147:190	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	0	23	theme	multifunctional	17:31	arg1	alcohol/starch					43:56	High-performance multifunctional polyvinyl alcohol/starch	0:56	High-performance multifunctional polyvinyl alcohol/starch	0:56	High-performance multifunctional polyvinyl alcohol/starch based active packaging films compatibilized with bioinspired polydopamine nanoparticles.					
35513104	2	24	theme	thermal	513:519	arg1	insulating					521:530	thermal insulating	513:530	thermal insulating	513:530	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	4	25	theme	thermal	929:935	arg1	stability					937:945	thermal stability	929:945	thermal stability	929:945	Compared with virgin PVA/ST film, the resulting PVA/ST/PDA nanocomposite films exhibited greatly improved tensile strength, toughness, thermal stability, and water vapor barrier ability.					
35513104	2	26	theme	PDA	386:388	arg1	addition					374:381	the addition	370:381	the addition of PDA	370:388	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	27	theme	vapor	441:445	arg1	barrier					447:453	water vapor barrier	435:453	water vapor barrier	435:453	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	28	theme	light	490:494	arg1	blocking					503:510	ultraviolet (UV)/high-energy blue light (HEBL) blocking	456:510	ultraviolet (UV)/high-energy blue light (HEBL) blocking	456:510	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	3	29	theme	compact	717:723	arg1	films					749:753	compact and dense nanocomposite films	717:753	compact and dense nanocomposite films	717:753	Results demonstrated that the added PDA nanoparticles were evenly dispersed in the PVA/ST matrix, providing compact and dense nanocomposite films due to their compatibilization effect.					
35513104	5	30	theme	PDA	1010:1012	arg1	presence					998:1005	the presence	994:1005	the presence of PDA	994:1012	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	2	31	theme	water	435:439	arg1	barrier					447:453	water vapor barrier	435:453	water vapor barrier	435:453	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	32	theme	blue	485:488	arg1	light					490:494	ultraviolet (UV)/high-energy blue light	456:494	ultraviolet (UV)/high-energy blue light (HEBL) blocking	456:510	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	32	theme	blue	485:488	arg1	HEBL					497:500	HEBL	497:500	HEBL	497:500	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	6	33	theme	high-performance	1141:1156	arg1	films					1188:1192	such high-performance multifunctional nanocomposite films	1136:1192	such high-performance multifunctional nanocomposite films	1136:1192	Thus, such high-performance multifunctional nanocomposite films hold the potential of protecting food quality against photothermal oxidative deterioration and extend food shelf life.					
35513104	4	34	theme	tensile	900:906	arg1	strength					908:915	greatly improved tensile strength	883:915	greatly improved tensile strength	883:915	Compared with virgin PVA/ST film, the resulting PVA/ST/PDA nanocomposite films exhibited greatly improved tensile strength, toughness, thermal stability, and water vapor barrier ability.					
35513104	1	35	theme	Bioinspired	147:157	arg1	PDA					173:175	PDA	173:175	PDA	173:175	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	1	35	theme	Bioinspired	147:157	arg1	polydopamine					159:170	Bioinspired polydopamine	147:170	Bioinspired polydopamine (PDA) nanoparticles	147:190	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	4	36	theme	improved	891:898	arg1	strength					908:915	greatly improved tensile strength	883:915	greatly improved tensile strength	883:915	Compared with virgin PVA/ST film, the resulting PVA/ST/PDA nanocomposite films exhibited greatly improved tensile strength, toughness, thermal stability, and water vapor barrier ability.					
35513104	1	37	theme	polyvinyl	255:263	arg1	matrix					289:294	polyvinyl alcohol/starch (PVA/ST) matrix	255:294	polyvinyl alcohol/starch (PVA/ST) matrix	255:294	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	0	38	theme	polyvinyl	33:41	arg1	alcohol/starch					43:56	High-performance multifunctional polyvinyl alcohol/starch	0:56	High-performance multifunctional polyvinyl alcohol/starch	0:56	High-performance multifunctional polyvinyl alcohol/starch based active packaging films compatibilized with bioinspired polydopamine nanoparticles.					
35513104	6	39	theme	food	1296:1299	arg1	life					1307:1310	food shelf life	1296:1310	food shelf life	1296:1310	Thus, such high-performance multifunctional nanocomposite films hold the potential of protecting food quality against photothermal oxidative deterioration and extend food shelf life.					
35513104	1	40	theme	polydopamine	159:170	arg1	compatibilizers					236:250	functional compatibilizers	225:250	functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix	225:294	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	1	40	theme	polydopamine	159:170	arg1	nanoparticles					178:190	Bioinspired polydopamine (PDA) nanoparticles	147:190	Bioinspired polydopamine (PDA) nanoparticles	147:190	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	1	41	theme	alcohol/starch	265:278	arg1	matrix					289:294	polyvinyl alcohol/starch (PVA/ST) matrix	255:294	polyvinyl alcohol/starch (PVA/ST) matrix	255:294	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	0	42	theme	active	64:69	arg1	films					81:85	active packaging films	64:85	active packaging films compatibilized with bioinspired polydopamine nanoparticles	64:144	High-performance multifunctional polyvinyl alcohol/starch based active packaging films compatibilized with bioinspired polydopamine nanoparticles.					
35513104	5	43	theme	thermal	1077:1083	arg1	blocking					1067:1074	excellent UV/HEBL blocking	1049:1074	excellent UV/HEBL blocking	1049:1074	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	5	43	theme	thermal	1077:1083	arg1	insulating					1085:1094	thermal insulating	1077:1094	thermal insulating as well as antioxidant functions	1077:1127	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	3	44	theme	PDA	645:647	arg1	nanoparticles					649:661	the added PDA nanoparticles	635:661	the added PDA nanoparticles	635:661	Results demonstrated that the added PDA nanoparticles were evenly dispersed in the PVA/ST matrix, providing compact and dense nanocomposite films due to their compatibilization effect.					
35513104	6	45	theme	shelf	1301:1305	arg1	life					1307:1310	food shelf life	1296:1310	food shelf life	1296:1310	Thus, such high-performance multifunctional nanocomposite films hold the potential of protecting food quality against photothermal oxidative deterioration and extend food shelf life.					
35513104	6	46	theme	oxidative	1261:1269	arg1	deterioration					1271:1283	photothermal oxidative deterioration	1248:1283	photothermal oxidative deterioration	1248:1283	Thus, such high-performance multifunctional nanocomposite films hold the potential of protecting food quality against photothermal oxidative deterioration and extend food shelf life.					
35513104	2	47	theme	films	579:583	arg1	barrier					447:453	water vapor barrier	435:453	water vapor barrier	435:453	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	47	theme	films	579:583	arg1	blocking					503:510	ultraviolet (UV)/high-energy blue light (HEBL) blocking	456:510	ultraviolet (UV)/high-energy blue light (HEBL) blocking	456:510	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	47	theme	films	579:583	arg1	insulating					521:530	thermal insulating	513:530	thermal insulating	513:530	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	47	theme	films	579:583	arg1	properties					548:557	antioxidant properties	536:557	antioxidant properties	536:557	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	48	theme	ultraviolet	456:466	arg1	light					490:494	ultraviolet (UV)/high-energy blue light	456:494	ultraviolet (UV)/high-energy blue light (HEBL) blocking	456:510	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	48	theme	ultraviolet	456:466	arg1	HEBL					497:500	HEBL	497:500	HEBL	497:500	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	4	49	theme	barrier	964:970	arg1	ability					972:978	water vapor barrier ability	952:978	water vapor barrier ability	952:978	Compared with virgin PVA/ST film, the resulting PVA/ST/PDA nanocomposite films exhibited greatly improved tensile strength, toughness, thermal stability, and water vapor barrier ability.					
35513104	3	50	theme	PVA/ST	692:697	arg1	matrix					699:704	the PVA/ST matrix	688:704	the PVA/ST matrix	688:704	Results demonstrated that the added PDA nanoparticles were evenly dispersed in the PVA/ST matrix, providing compact and dense nanocomposite films due to their compatibilization effect.					
35513104	6	51	theme	food	1227:1230	arg1	quality					1232:1238	food quality	1227:1238	food quality	1227:1238	Thus, such high-performance multifunctional nanocomposite films hold the potential of protecting food quality against photothermal oxidative deterioration and extend food shelf life.					
35513104	0	52	theme	packaging	71:79	arg1	films					81:85	active packaging films	64:85	active packaging films compatibilized with bioinspired polydopamine nanoparticles	64:144	High-performance multifunctional polyvinyl alcohol/starch based active packaging films compatibilized with bioinspired polydopamine nanoparticles.					
35513104	4	53	theme	vapor	958:962	arg1	ability					972:978	water vapor barrier ability	952:978	water vapor barrier ability	952:978	Compared with virgin PVA/ST film, the resulting PVA/ST/PDA nanocomposite films exhibited greatly improved tensile strength, toughness, thermal stability, and water vapor barrier ability.					
35513104	5	54	theme	antioxidant	1107:1117	arg1	functions					1119:1127	antioxidant functions	1107:1127	thermal insulating as well as antioxidant functions	1077:1127	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	5	54	theme	antioxidant	1107:1117	arg1	blocking					1067:1074	excellent UV/HEBL blocking	1049:1074	excellent UV/HEBL blocking	1049:1074	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	3	55	theme	added	639:643	arg1	nanoparticles					649:661	the added PDA nanoparticles	635:661	the added PDA nanoparticles	635:661	Results demonstrated that the added PDA nanoparticles were evenly dispersed in the PVA/ST matrix, providing compact and dense nanocomposite films due to their compatibilization effect.					
35513104	2	56	theme	microstructural	397:411	arg1	barrier					447:453	water vapor barrier	435:453	water vapor barrier	435:453	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	57	dep	barrier	447:453	arg1	the					393:395	the	393:395	the	393:395	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	3	58	theme	dense	729:733	arg1	films					749:753	compact and dense nanocomposite films	717:753	compact and dense nanocomposite films	717:753	Results demonstrated that the added PDA nanoparticles were evenly dispersed in the PVA/ST matrix, providing compact and dense nanocomposite films due to their compatibilization effect.					
35513104	4	59	theme	nanocomposite	853:865	arg1	films					867:871	the resulting PVA/ST/PDA nanocomposite films	828:871	the resulting PVA/ST/PDA nanocomposite films	828:871	Compared with virgin PVA/ST film, the resulting PVA/ST/PDA nanocomposite films exhibited greatly improved tensile strength, toughness, thermal stability, and water vapor barrier ability.					
35513104	2	60	theme	addition	374:381	arg1	effect					360:365	The effect	356:365	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films	356:583	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	5	61	theme	excellent	1049:1057	arg1	blocking					1067:1074	excellent UV/HEBL blocking	1049:1074	excellent UV/HEBL blocking	1049:1074	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	5	61	theme	excellent	1049:1057	arg1	insulating					1085:1094	thermal insulating	1077:1094	thermal insulating as well as antioxidant functions	1077:1127	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	5	61	theme	excellent	1049:1057	arg1	functions					1119:1127	antioxidant functions	1107:1127	thermal insulating as well as antioxidant functions	1077:1127	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	4	62	theme	PVA/ST/PDA	842:851	arg1	films					867:871	the resulting PVA/ST/PDA nanocomposite films	828:871	the resulting PVA/ST/PDA nanocomposite films	828:871	Compared with virgin PVA/ST film, the resulting PVA/ST/PDA nanocomposite films exhibited greatly improved tensile strength, toughness, thermal stability, and water vapor barrier ability.					
35513104	1	63	from	compatibilizers	236:250	arg1	matrix					289:294	polyvinyl alcohol/starch (PVA/ST) matrix	255:294	polyvinyl alcohol/starch (PVA/ST) matrix	255:294	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	2	64	theme	antioxidant	536:546	arg1	properties					548:557	antioxidant properties	536:557	antioxidant properties	536:557	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	1	65	theme	PVA/ST	281:286	arg1	matrix					289:294	polyvinyl alcohol/starch (PVA/ST) matrix	255:294	polyvinyl alcohol/starch (PVA/ST) matrix	255:294	Bioinspired polydopamine (PDA) nanoparticles were synthesized and explored as functional compatibilizers in polyvinyl alcohol/starch (PVA/ST) matrix to develop high-performance multifunctional packaging film.					
35513104	5	66	theme	PVA/ST	1022:1027	arg1	film					1039:1042	PVA/ST composite film	1022:1042	PVA/ST composite film	1022:1042	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	2	67	from	effect	360:365	arg1	barrier					447:453	water vapor barrier	435:453	water vapor barrier	435:453	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	67	from	effect	360:365	arg1	blocking					503:510	ultraviolet (UV)/high-energy blue light (HEBL) blocking	456:510	ultraviolet (UV)/high-energy blue light (HEBL) blocking	456:510	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	67	from	effect	360:365	arg1	insulating					521:530	thermal insulating	513:530	thermal insulating	513:530	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	67	from	effect	360:365	arg1	properties					548:557	antioxidant properties	536:557	antioxidant properties	536:557	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	5	68	theme	UV/HEBL	1059:1065	arg1	blocking					1067:1074	excellent UV/HEBL blocking	1049:1074	excellent UV/HEBL blocking	1049:1074	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	5	68	theme	UV/HEBL	1059:1065	arg1	insulating					1085:1094	thermal insulating	1077:1094	thermal insulating as well as antioxidant functions	1077:1127	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	5	68	theme	UV/HEBL	1059:1065	arg1	functions					1119:1127	antioxidant functions	1107:1127	thermal insulating as well as antioxidant functions	1077:1127	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	2	69	theme	/high-energy	472:483	arg1	light					490:494	ultraviolet (UV)/high-energy blue light	456:494	ultraviolet (UV)/high-energy blue light (HEBL) blocking	456:510	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	2	69	theme	/high-energy	472:483	arg1	HEBL					497:500	HEBL	497:500	HEBL	497:500	The effect of the addition of PDA on the microstructural, mechanical, thermal, water vapor barrier, ultraviolet (UV)/high-energy blue light (HEBL) blocking, thermal insulating and antioxidant properties of PVA/ST composite films was fully investigated.					
35513104	5	70	with	endowed	1014:1020	arg1	blocking					1067:1074	excellent UV/HEBL blocking	1049:1074	excellent UV/HEBL blocking	1049:1074	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	5	70	with	endowed	1014:1020	arg1	insulating					1085:1094	thermal insulating	1077:1094	thermal insulating as well as antioxidant functions	1077:1127	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35513104	5	70	with	endowed	1014:1020	arg1	functions					1119:1127	antioxidant functions	1107:1127	thermal insulating as well as antioxidant functions	1077:1127	Furthermore, the presence of PDA endowed PVA/ST composite film with excellent UV/HEBL blocking, thermal insulating as well as antioxidant functions.					
35349085	4	0	theme	optimized	865:873	arg1	conditions					875:884	The optimized conditions	861:884	The optimized conditions	861:884	The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL- 1 laccases, and 2.44 h resulting in 547 ± 108 mg L- 1 of total sugars.					
35349085	4	0	theme	optimized	865:873	arg1	SCB					903:905	23.4 g L- 1 SCB	891:905	23.4 g L- 1 SCB	891:905	The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL- 1 laccases, and 2.44 h resulting in 547 ± 108 mg L- 1 of total sugars.					
35349085	2	1	attach	isolated	455:462	arg2	Lacmix					447:452	Lacmix	447:452	Lacmix	447:452	This study was undertaken to explore the applicability of a new extract with the enzymes (Lacmix) isolated from Chaetomium cupreum for SCB pretreatment.					
35349085	2	1	attach	isolated	455:462	arg2	enzymes					438:444	the enzymes	434:444	the enzymes (Lacmix) isolated from Chaetomium cupreum for SCB pretreatment	434:507	This study was undertaken to explore the applicability of a new extract with the enzymes (Lacmix) isolated from Chaetomium cupreum for SCB pretreatment.					
35349085	2	1	attach	isolated	455:462	arg1	cupreum					480:486	Chaetomium cupreum	469:486	Chaetomium cupreum	469:486	This study was undertaken to explore the applicability of a new extract with the enzymes (Lacmix) isolated from Chaetomium cupreum for SCB pretreatment.					
35349085	4	2	theme	mL- 1	916:920	arg1	laccases					922:929	0.234 U mL- 1 laccases	908:929	0.234 U mL- 1 laccases	908:929	The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL- 1 laccases, and 2.44 h resulting in 547 ± 108 mg L- 1 of total sugars.					
35349085	1	3	theme	environmentally	227:241	arg1	approach					252:259	an environmentally friendly approach	224:259	an environmentally friendly approach	224:259	The production of biofuels using sugarcane bagasse (SCB) as substrate can be considered an environmentally friendly approach, due to the possibility of combining energy production with the reuse of agroindustrial wastes.					
35349085	3	4	theme	concentration	618:630	arg1	effects					603:609	the individual and mutual effects	577:609	the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment	577:777	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	1	5	theme	biofuels	154:161	arg1	production					140:149	The production	136:149	The production of biofuels using sugarcane bagasse (SCB) as substrate	136:204	The production of biofuels using sugarcane bagasse (SCB) as substrate can be considered an environmentally friendly approach, due to the possibility of combining energy production with the reuse of agroindustrial wastes.					
35349085	1	6	theme	friendly	243:250	arg1	approach					252:259	an environmentally friendly approach	224:259	an environmentally friendly approach	224:259	The production of biofuels using sugarcane bagasse (SCB) as substrate can be considered an environmentally friendly approach, due to the possibility of combining energy production with the reuse of agroindustrial wastes.					
35349085	7	7	theme	biomass	1283:1289	arg1	fermentation					1291:1302	biomass fermentation	1283:1302	biomass fermentation for biofuel production	1283:1325	Therefore, laccases improve delignification, maximizing biomass fermentation for biofuel production.					
35349085	3	8	theme	time	715:718	arg1	effects					603:609	the individual and mutual effects	577:609	the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment	577:777	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	2	9	theme	SCB	492:494	arg1	pretreatment					496:507	SCB pretreatment	492:507	SCB pretreatment	492:507	This study was undertaken to explore the applicability of a new extract with the enzymes (Lacmix) isolated from Chaetomium cupreum for SCB pretreatment.					
35349085	1	10	theme	agroindustrial	334:347	arg1	wastes					349:354	agroindustrial wastes	334:354	agroindustrial wastes	334:354	The production of biofuels using sugarcane bagasse (SCB) as substrate can be considered an environmentally friendly approach, due to the possibility of combining energy production with the reuse of agroindustrial wastes.					
35349085	6	11	dep	%	1154:1154	arg1	acids					1169:1173	more organic acids	1156:1173	22.5% more organic acids	1150:1173	Through the SCB pretreated with Lacmix fermentation, 96.1% more H2 and 22.5% more organic acids were observed compared to SCB without pretreatment.					
35349085	0	12	theme	H2	114:115	arg1	Production					100:109	the Production	96:109	the Production of H2 and Organic Acids	96:133	The Deconstruction of the Lignocellulolytic Structure of Sugarcane Bagasse by Laccases Improves the Production of H2 and Organic Acids.					
35349085	1	13	theme	wastes	349:354	arg1	reuse					325:329	the reuse	321:329	the reuse of agroindustrial wastes	321:354	The production of biofuels using sugarcane bagasse (SCB) as substrate can be considered an environmentally friendly approach, due to the possibility of combining energy production with the reuse of agroindustrial wastes.					
35349085	4	14	theme	547 ± 108 mg	956:967	arg1	L- 1					969:972	547 ± 108 mg L- 1	956:972	547 ± 108 mg L- 1 of total sugars	956:988	The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL- 1 laccases, and 2.44 h resulting in 547 ± 108 mg L- 1 of total sugars.					
35349085	6	15	theme	Lacmix	1111:1116	arg1	fermentation					1118:1129	Lacmix fermentation	1111:1129	Lacmix fermentation	1111:1129	Through the SCB pretreated with Lacmix fermentation, 96.1% more H2 and 22.5% more organic acids were observed compared to SCB without pretreatment.					
35349085	3	16	theme	SCB	762:764	arg1	pretreatment					766:777	SCB pretreatment	762:777	SCB pretreatment	762:777	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	1	17	theme	sugarcane	169:177	arg1	bagasse					179:185	sugarcane bagasse	169:185	sugarcane bagasse (SCB)	169:191	The production of biofuels using sugarcane bagasse (SCB) as substrate can be considered an environmentally friendly approach, due to the possibility of combining energy production with the reuse of agroindustrial wastes.					
35349085	1	17	theme	sugarcane	169:177	arg1	SCB					188:190	SCB	188:190	SCB	188:190	The production of biofuels using sugarcane bagasse (SCB) as substrate can be considered an environmentally friendly approach, due to the possibility of combining energy production with the reuse of agroindustrial wastes.					
35349085	3	18	dep	L- 1	693:696	arg1	to					682:683	to	682:683	to	682:683	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	19	theme	SCB	727:729	arg1	L- 1					647:650	6.6 to 23.4 g L- 1	633:650	6.6 to 23.4 g L- 1	633:650	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	19	theme	SCB	727:729	arg1	concentration					618:630	SCB concentration	614:630	SCB concentration (6.6 to 23.4 g L- 1)	614:651	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	19	theme	SCB	727:729	arg1	concentration					661:673	enzyme concentration	654:673	enzyme concentration (0.066 to 0.234 U L- 1)	654:697	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	19	theme	SCB	727:729	arg1	time					715:718	incubation time	704:718	incubation time of the SCB with Lacmix (19 to 221 min)	704:757	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	19	theme	SCB	727:729	arg1	L- 1					693:696	0.066 to 0.234 U L- 1	676:696	0.066 to 0.234 U L- 1	676:696	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	20	dep	221 min	750:756	arg1	to					747:748	to	747:748	to	747:748	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	21	dep	4	549:549	arg1	to					546:547	to	546:547	to	546:547	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	2	22	theme	extract	421:427	arg1	applicability					398:410	the applicability	394:410	the applicability of a new extract with the enzymes (Lacmix) isolated from Chaetomium cupreum for SCB pretreatment	394:507	This study was undertaken to explore the applicability of a new extract with the enzymes (Lacmix) isolated from Chaetomium cupreum for SCB pretreatment.					
35349085	4	23	theme	L- 1	898:901	arg1	conditions					875:884	The optimized conditions	861:884	The optimized conditions	861:884	The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL- 1 laccases, and 2.44 h resulting in 547 ± 108 mg L- 1 of total sugars.					
35349085	4	23	theme	L- 1	898:901	arg1	SCB					903:905	23.4 g L- 1 SCB	891:905	23.4 g L- 1 SCB	891:905	The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL- 1 laccases, and 2.44 h resulting in 547 ± 108 mg L- 1 of total sugars.					
35349085	3	24	theme	response	802:809	arg1	methodology					819:829	a response surface methodology	800:829	a response surface methodology	800:829	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	2	25	theme	new	417:419	arg1	extract					421:427	a new extract	415:427	a new extract with the enzymes (Lacmix) isolated from Chaetomium cupreum for SCB pretreatment	415:507	This study was undertaken to explore the applicability of a new extract with the enzymes (Lacmix) isolated from Chaetomium cupreum for SCB pretreatment.					
35349085	5	26	theme	455 ± 41 mg	1035:1045	arg1	value					1028:1032	the predicted value	1014:1032	the predicted value (455 ± 41 mg L- 1)	1014:1051	This value agrees with the predicted value (455 ± 41 mg L- 1) by the statistical model.					
35349085	5	26	theme	455 ± 41 mg	1035:1045	arg1	L- 1					1047:1050	455 ± 41 mg L- 1	1035:1050	455 ± 41 mg L- 1	1035:1050	This value agrees with the predicted value (455 ± 41 mg L- 1) by the statistical model.					
35349085	0	27	theme	Organic	121:127	arg1	Acids					129:133	Organic Acids	121:133	Organic Acids	121:133	The Deconstruction of the Lignocellulolytic Structure of Sugarcane Bagasse by Laccases Improves the Production of H2 and Organic Acids.					
35349085	3	28	theme	surface	811:817	arg1	methodology					819:829	a response surface methodology	800:829	a response surface methodology	800:829	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	4	29	theme	0.234	908:912	arg1	laccases					922:929	0.234 U mL- 1 laccases	908:929	0.234 U mL- 1 laccases	908:929	The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL- 1 laccases, and 2.44 h resulting in 547 ± 108 mg L- 1 of total sugars.					
35349085	3	30	theme	enzyme	654:659	arg1	concentration					661:673	enzyme concentration	654:673	enzyme concentration (0.066 to 0.234 U L- 1)	654:697	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	30	theme	enzyme	654:659	arg1	L- 1					693:696	0.066 to 0.234 U L- 1	676:696	0.066 to 0.234 U L- 1	676:696	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	31	theme	individual	581:590	arg1	effects					603:609	the individual and mutual effects	577:609	the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment	577:777	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	4	32	theme	sugars	983:988	arg1	L- 1					969:972	547 ± 108 mg L- 1	956:972	547 ± 108 mg L- 1 of total sugars	956:988	The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL- 1 laccases, and 2.44 h resulting in 547 ± 108 mg L- 1 of total sugars.					
35349085	5	33	theme	predicted	1018:1026	arg1	value					1028:1032	the predicted value	1014:1032	the predicted value (455 ± 41 mg L- 1)	1014:1051	This value agrees with the predicted value (455 ± 41 mg L- 1) by the statistical model.					
35349085	5	33	theme	predicted	1018:1026	arg1	L- 1					1047:1050	455 ± 41 mg L- 1	1035:1050	455 ± 41 mg L- 1	1035:1050	This value agrees with the predicted value (455 ± 41 mg L- 1) by the statistical model.					
35349085	0	34	theme	Structure	44:52	arg1	Deconstruction					4:17	The Deconstruction	0:17	The Deconstruction of the Lignocellulolytic Structure of Sugarcane Bagasse by Laccases	0:85	The Deconstruction of the Lignocellulolytic Structure of Sugarcane Bagasse by Laccases Improves the Production of H2 and Organic Acids.					
35349085	3	35	theme	concentration	661:673	arg1	effects					603:609	the individual and mutual effects	577:609	the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment	577:777	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	6	36	theme	organic	1161:1167	arg1	acids					1169:1173	more organic acids	1156:1173	22.5% more organic acids	1150:1173	Through the SCB pretreated with Lacmix fermentation, 96.1% more H2 and 22.5% more organic acids were observed compared to SCB without pretreatment.					
35349085	3	37	theme	4	549:549	arg1	pH					536:537	pH	536:537	pH of 2.2 to 4 and 50	536:556	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	4	38	theme	total	977:981	arg1	sugars					983:988	total sugars	977:988	total sugars	977:988	The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL- 1 laccases, and 2.44 h resulting in 547 ± 108 mg L- 1 of total sugars.					
35349085	0	39	theme	Lignocellulolytic	26:42	arg1	Structure					44:52	the Lignocellulolytic Structure	22:52	the Lignocellulolytic Structure of Sugarcane Bagasse	22:73	The Deconstruction of the Lignocellulolytic Structure of Sugarcane Bagasse by Laccases Improves the Production of H2 and Organic Acids.					
35349085	3	40	theme	U	691:691	arg1	concentration					661:673	enzyme concentration	654:673	enzyme concentration (0.066 to 0.234 U L- 1)	654:697	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	40	theme	U	691:691	arg1	L- 1					693:696	0.066 to 0.234 U L- 1	676:696	0.066 to 0.234 U L- 1	676:696	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	0	41	theme	Acids	129:133	arg1	Production					100:109	the Production	96:109	the Production of H2 and Organic Acids	96:133	The Deconstruction of the Lignocellulolytic Structure of Sugarcane Bagasse by Laccases Improves the Production of H2 and Organic Acids.					
35349085	4	42	theme	U	914:914	arg1	laccases					922:929	0.234 U mL- 1 laccases	908:929	0.234 U mL- 1 laccases	908:929	The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL- 1 laccases, and 2.44 h resulting in 547 ± 108 mg L- 1 of total sugars.					
35349085	3	43	theme	central	835:841	arg1	design					853:858	central composite design	835:858	central composite design	835:858	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	6	44	theme	H2	1143:1144	arg1	%					1136:1136	96.1%	1132:1136	96.1% more H2	1132:1144	Through the SCB pretreated with Lacmix fermentation, 96.1% more H2 and 22.5% more organic acids were observed compared to SCB without pretreatment.					
35349085	0	45	theme	Sugarcane	57:65	arg1	Bagasse					67:73	Sugarcane Bagasse	57:73	Sugarcane Bagasse	57:73	The Deconstruction of the Lignocellulolytic Structure of Sugarcane Bagasse by Laccases Improves the Production of H2 and Organic Acids.					
35349085	3	46	dep	23.4 g	640:645	arg1	to					637:638	to	637:638	to	637:638	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	47	from	effects	603:609	arg1	pretreatment					766:777	SCB pretreatment	762:777	SCB pretreatment	762:777	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	48	theme	composite	843:851	arg1	design					853:858	central composite design	835:858	central composite design	835:858	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	4	49	theme	23.4 g	891:896	arg1	conditions					875:884	The optimized conditions	861:884	The optimized conditions	861:884	The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL- 1 laccases, and 2.44 h resulting in 547 ± 108 mg L- 1 of total sugars.					
35349085	4	49	theme	23.4 g	891:896	arg1	SCB					903:905	23.4 g L- 1 SCB	891:905	23.4 g L- 1 SCB	891:905	The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL- 1 laccases, and 2.44 h resulting in 547 ± 108 mg L- 1 of total sugars.					
35349085	1	50	theme	energy	298:303	arg1	production					305:314	energy production	298:314	energy production	298:314	The production of biofuels using sugarcane bagasse (SCB) as substrate can be considered an environmentally friendly approach, due to the possibility of combining energy production with the reuse of agroindustrial wastes.					
35349085	3	51	theme	0.234	685:689	arg1	concentration					661:673	enzyme concentration	654:673	enzyme concentration (0.066 to 0.234 U L- 1)	654:697	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	51	theme	0.234	685:689	arg1	L- 1					693:696	0.066 to 0.234 U L- 1	676:696	0.066 to 0.234 U L- 1	676:696	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	52	theme	SCB	614:616	arg1	L- 1					647:650	6.6 to 23.4 g L- 1	633:650	6.6 to 23.4 g L- 1	633:650	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	52	theme	SCB	614:616	arg1	concentration					618:630	SCB concentration	614:630	SCB concentration (6.6 to 23.4 g L- 1)	614:651	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	5	53	theme	statistical	1060:1070	arg1	model					1072:1076	the statistical model	1056:1076	the statistical model	1056:1076	This value agrees with the predicted value (455 ± 41 mg L- 1) by the statistical model.					
35349085	2	54	with	extract	421:427	arg1	enzymes					438:444	the enzymes	434:444	the enzymes (Lacmix) isolated from Chaetomium cupreum for SCB pretreatment	434:507	This study was undertaken to explore the applicability of a new extract with the enzymes (Lacmix) isolated from Chaetomium cupreum for SCB pretreatment.					
35349085	2	54	with	extract	421:427	arg1	Lacmix					447:452	Lacmix	447:452	Lacmix	447:452	This study was undertaken to explore the applicability of a new extract with the enzymes (Lacmix) isolated from Chaetomium cupreum for SCB pretreatment.					
35349085	3	55	with	SCB	727:729	arg1	221 min					750:756	19 to 221 min	744:756	19 to 221 min	744:756	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	55	with	SCB	727:729	arg1	Lacmix					736:741	Lacmix	736:741	Lacmix (19 to 221 min)	736:757	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	56	theme	mutual	596:601	arg1	effects					603:609	the individual and mutual effects	577:609	the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment	577:777	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	57	theme	Further	568:574	arg1	60 °C.					561:566	60 °C. Further	561:574	60 °C. Further	561:574	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	58	from	pH	536:537	arg1	active					526:531	active	526:531	active	526:531	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	59	theme	incubation	704:713	arg1	time					715:718	incubation time	704:718	incubation time of the SCB with Lacmix (19 to 221 min)	704:757	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	3	60	theme	50	555:556	arg1	pH					536:537	pH	536:537	pH of 2.2 to 4 and 50	536:556	Lacmix was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lacmix (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design.					
35349085	0	61	theme	Bagasse	67:73	arg1	Structure					44:52	the Lignocellulolytic Structure	22:52	the Lignocellulolytic Structure of Sugarcane Bagasse	22:73	The Deconstruction of the Lignocellulolytic Structure of Sugarcane Bagasse by Laccases Improves the Production of H2 and Organic Acids.					
35349085	7	62	theme	biofuel	1308:1314	arg1	production					1316:1325	biofuel production	1308:1325	biofuel production	1308:1325	Therefore, laccases improve delignification, maximizing biomass fermentation for biofuel production.					
37244340	0	0	theme	citric	77:82	arg1	acid					84:87	citric acid	77:87	citric acid	77:87	Preparation and evaluation of a novel chlorine dioxide preservative based on citric acid grafted carboxymethyl chitosan.					
37244340	6	1	theme	CK	1154:1155	arg1	group					1157:1161	the CK group	1150:1161	the CK group (0 g ClO2 preservative)	1150:1185	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	6	1	theme	CK	1154:1155	arg1	ClO2					1168:1171	0 g ClO2 preservative	1164:1184	0 g ClO2 preservative	1164:1184	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	7	2	contain	had	1258:1260	arg2	rate					1315:1318	lowest respiration rate	1296:1318	lowest respiration rate of 34.42 mg·kg-1·h-1	1296:1339	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	7	2	contain	had	1258:1260	arg2	value					1277:1281	the highest L* value	1262:1281	the highest L* value of 47.47	1262:1290	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	7	2	contain	had	1258:1260	arg1	longan					1214:1219	longan	1214:1219	longan treated with 0.3 g ClO2 preservative	1214:1256	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	2	3	theme	acid	328:331	arg1	substituents					338:349	citric acid (CA) substituents	321:349	citric acid (CA) substituents	321:349	In this study, a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents were synthesized, characterized, and further used to prepare a novel ClO2 slow-releasing preservative for longan.					
37244340	4	4	theme	Further	543:549	arg1	titration					566:574	Further potentiometric titration	543:574	Further potentiometric titration	543:574	Further potentiometric titration showed that the mass ratios of CA grafted in CMC-CA#1-3 were 0.18:1, 0.42:1, and 0.42:1, respectively.					
37244340	5	5	theme	ClO2	716:719	arg1	slow-releasing					721:734	ClO2 slow-releasing preservative	716:747	ClO2 slow-releasing preservative	716:747	The composition and concentration of ClO2 slow-releasing preservative were optimized, and the best formulation was as follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1.					
37244340	5	6	theme	slow-releasing	721:734	arg1	concentration					699:711	concentration	699:711	concentration	699:711	The composition and concentration of ClO2 slow-releasing preservative were optimized, and the best formulation was as follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1.					
37244340	5	6	theme	slow-releasing	721:734	arg1	composition					683:693	composition	683:693	composition	683:693	The composition and concentration of ClO2 slow-releasing preservative were optimized, and the best formulation was as follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1.					
37244340	2	7	theme	citric	321:326	arg1	acid					328:331	citric acid	321:331	citric acid (CA) substituents	321:349	In this study, a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents were synthesized, characterized, and further used to prepare a novel ClO2 slow-releasing preservative for longan.					
37244340	2	7	theme	citric	321:326	arg1	CA					334:335	CA	334:335	CA	334:335	In this study, a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents were synthesized, characterized, and further used to prepare a novel ClO2 slow-releasing preservative for longan.					
37244340	6	8	theme	ClO2	1013:1016	arg1	preservative					1018:1029	0.15-1.2 g ClO2 preservative	1002:1029	0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative)	1002:1185	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	7	9	theme	ClO2	1240:1243	arg1	preservative					1245:1256	0.3 g ClO2 preservative	1234:1256	0.3 g ClO2 preservative	1234:1256	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	6	10	theme	0 g	1164:1166	arg1	group					1157:1161	the CK group	1150:1161	the CK group (0 g ClO2 preservative)	1150:1185	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	6	10	theme	0 g	1164:1166	arg1	ClO2					1168:1171	0 g ClO2 preservative	1164:1184	0 g ClO2 preservative	1164:1184	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	3	11	theme	CMC-CA	504:509	arg1	#					510:510	CMC-CA#1-3	504:513	CMC-CA#1-3	504:513	UV-Vis and FT-IR spectra revealed that CMC-CA#1-3 were successfully prepared.					
37244340	6	12	theme	higher	1060:1065	arg1	values					1077:1082	significantly (p < 0.05) higher L* and a* values	1035:1082	significantly (p < 0.05) higher L* and a* values	1035:1082	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	2	13	with	chitosan	301:308	arg1	substituents					338:349	citric acid (CA) substituents	321:349	citric acid (CA) substituents	321:349	In this study, a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents were synthesized, characterized, and further used to prepare a novel ClO2 slow-releasing preservative for longan.					
37244340	7	14	theme	34.42 mg·kg-1·h-1	1323:1339	arg1	value					1277:1281	the highest L* value	1262:1281	the highest L* value of 47.47	1262:1290	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	7	14	theme	34.42 mg·kg-1·h-1	1323:1339	arg1	rate					1315:1318	lowest respiration rate	1296:1318	lowest respiration rate of 34.42 mg·kg-1·h-1	1296:1339	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	7	15	theme	respiration	1303:1313	arg1	rate					1315:1318	lowest respiration rate	1296:1318	lowest respiration rate of 34.42 mg·kg-1·h-1	1296:1339	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	3	16	theme	UV-Vis	465:470	arg1	spectra					482:488	UV-Vis and FT-IR spectra	465:488	spectra	482:488	UV-Vis and FT-IR spectra revealed that CMC-CA#1-3 were successfully prepared.					
37244340	4	17	theme	potentiometric	551:564	arg1	titration					566:574	Further potentiometric titration	543:574	Further potentiometric titration	543:574	Further potentiometric titration showed that the mass ratios of CA grafted in CMC-CA#1-3 were 0.18:1, 0.42:1, and 0.42:1, respectively.					
37244340	2	18	used	used	396:399	arg2	series					277:282	a series	275:282	a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents	275:349	In this study, a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents were synthesized, characterized, and further used to prepare a novel ClO2 slow-releasing preservative for longan.					
37244340	6	19	theme	*	1075:1075	arg1	values					1077:1082	significantly (p < 0.05) higher L* and a* values	1035:1082	significantly (p < 0.05) higher L* and a* values	1035:1082	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	4	20	theme	CMC-CA	621:626	arg1	#					627:627	CMC-CA#1-3	621:630	CMC-CA#1-3	621:630	Further potentiometric titration showed that the mass ratios of CA grafted in CMC-CA#1-3 were 0.18:1, 0.42:1, and 0.42:1, respectively.					
37244340	0	21	theme	carboxymethyl	97:109	arg1	chitosan					111:118	carboxymethyl chitosan	97:118	carboxymethyl chitosan	97:118	Preparation and evaluation of a novel chlorine dioxide preservative based on citric acid grafted carboxymethyl chitosan.					
37244340	8	22	theme	longan	1455:1460	arg1	preservation					1462:1473	longan preservation	1455:1473	longan preservation	1455:1473	This study provided a safe, effective, and simple solution for longan preservation.					
37244340	0	23	theme	novel	32:36	arg1	preservative					55:66	a novel chlorine dioxide preservative	30:66	a novel chlorine dioxide preservative based on citric acid	30:87	Preparation and evaluation of a novel chlorine dioxide preservative based on citric acid grafted carboxymethyl chitosan.					
37244340	6	24	dep	higher	1060:1065	arg1	p < 0.05					1050:1057	p < 0.05	1050:1057	p < 0.05	1050:1057	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	7	25	theme	storage	1205:1211	arg1	17 days					1194:1200	17 days	1194:1200	17 days of storage	1194:1211	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	8	26	theme	safe	1414:1417	arg1	solution					1442:1449	a safe, effective, and simple solution	1412:1449	a safe, effective, and simple solution for longan preservation	1412:1473	This study provided a safe, effective, and simple solution for longan preservation.					
37244340	2	27	theme	chitosan	301:308	arg1	series					277:282	a series	275:282	a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents	275:349	In this study, a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents were synthesized, characterized, and further used to prepare a novel ClO2 slow-releasing preservative for longan.					
37244340	6	28	theme	release	941:947	arg1	rate					949:952	the maximum release rate	929:952	the maximum release rate	929:952	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	7	29	theme	lowest	1296:1301	arg1	rate					1315:1318	lowest respiration rate	1296:1318	lowest respiration rate of 34.42 mg·kg-1·h-1	1296:1339	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	7	30	theme	pericarp	1359:1366	arg1	color					1368:1372	pericarp color	1359:1372	pericarp color	1359:1372	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	2	31	theme	carboxymethyl	287:299	arg1	CMC					311:313	CMC	311:313	CMC	311:313	In this study, a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents were synthesized, characterized, and further used to prepare a novel ClO2 slow-releasing preservative for longan.					
37244340	2	31	theme	carboxymethyl	287:299	arg1	chitosan					301:308	carboxymethyl chitosan	287:308	carboxymethyl chitosan (CMC) with citric acid (CA) substituents	287:349	In this study, a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents were synthesized, characterized, and further used to prepare a novel ClO2 slow-releasing preservative for longan.					
37244340	5	32	theme	CMC-CA	813:818	arg1	#					819:819	CMC-CA#2	813:820	follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1	797:844	The composition and concentration of ClO2 slow-releasing preservative were optimized, and the best formulation was as follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1.					
37244340	6	33	theme	preservative	885:896	arg1	time					872:875	The maximum ClO2 release time	847:875	The maximum ClO2 release time of this preservative	847:896	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	6	34	theme	total	1115:1119	arg1	counts					1138:1143	total microbial colony counts	1115:1143	total microbial colony counts	1115:1143	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	6	35	theme	L	1067:1067	arg1	*					1068:1068	L*	1067:1068	L*	1067:1068	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	5	36	theme	best	773:776	arg1	formulation					778:788	the best formulation	769:788	the best formulation	769:788	The composition and concentration of ClO2 slow-releasing preservative were optimized, and the best formulation was as follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1.					
37244340	8	37	theme	effective	1420:1428	arg1	solution					1442:1449	a safe, effective, and simple solution	1412:1449	a safe, effective, and simple solution for longan preservation	1412:1473	This study provided a safe, effective, and simple solution for longan preservation.					
37244340	7	38	theme	47.47	1286:1290	arg1	value					1277:1281	the highest L* value	1262:1281	the highest L* value of 47.47	1262:1290	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	7	38	theme	47.47	1286:1290	arg1	rate					1315:1318	lowest respiration rate	1296:1318	lowest respiration rate of 34.42 mg·kg-1·h-1	1296:1339	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	6	39	theme	0.15-1.2 g	1002:1011	arg1	preservative					1018:1029	0.15-1.2 g ClO2 preservative	1002:1029	0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative)	1002:1185	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	6	40	theme	*	1068:1068	arg1	values					1077:1082	significantly (p < 0.05) higher L* and a* values	1035:1082	significantly (p < 0.05) higher L* and a* values	1035:1082	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	2	41	theme	slow-releasing	425:438	arg1	preservative					440:451	a novel ClO2 slow-releasing preservative	412:451	a novel ClO2 slow-releasing preservative for longan	412:462	In this study, a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents were synthesized, characterized, and further used to prepare a novel ClO2 slow-releasing preservative for longan.					
37244340	8	42	theme	simple	1435:1440	arg1	solution					1442:1449	a safe, effective, and simple solution	1412:1449	a safe, effective, and simple solution for longan preservation	1412:1473	This study provided a safe, effective, and simple solution for longan preservation.					
37244340	6	43	theme	12-36 h.	973:980	arg1	Longan					982:987	12-36 h. Longan	973:987	12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative)	973:1185	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	1	44	theme	great	209:213	arg1	attention					215:223	great attention	209:223	great attention due to its convenience and safety	209:257	Instrument-free chlorine dioxide (ClO2) preservative for fruit and vegetable has gained great attention due to its convenience and safety.					
37244340	2	45	theme	ClO2	420:423	arg1	preservative					440:451	a novel ClO2 slow-releasing preservative	412:451	a novel ClO2 slow-releasing preservative for longan	412:462	In this study, a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents were synthesized, characterized, and further used to prepare a novel ClO2 slow-releasing preservative for longan.					
37244340	6	46	theme	colony	1131:1136	arg1	counts					1138:1143	total microbial colony counts	1115:1143	total microbial colony counts	1115:1143	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	4	47	theme	CA	607:608	arg1	ratios					597:602	the mass ratios	588:602	the mass ratios of CA grafted in CMC-CA#1-3	588:630	Further potentiometric titration showed that the mass ratios of CA grafted in CMC-CA#1-3 were 0.18:1, 0.42:1, and 0.42:1, respectively.					
37244340	4	47	theme	CA	607:608	arg1	0.18:1					637:642	0.18:1	637:642	0.18:1	637:642	Further potentiometric titration showed that the mass ratios of CA grafted in CMC-CA#1-3 were 0.18:1, 0.42:1, and 0.42:1, respectively.					
37244340	0	48	theme	dioxide	47:53	arg1	preservative					55:66	a novel chlorine dioxide preservative	30:66	a novel chlorine dioxide preservative based on citric acid	30:87	Preparation and evaluation of a novel chlorine dioxide preservative based on citric acid grafted carboxymethyl chitosan.					
37244340	6	49	theme	release	864:870	arg1	time					872:875	The maximum ClO2 release time	847:875	The maximum ClO2 release time of this preservative	847:896	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	0	50	theme	preservative	55:66	arg1	evaluation					16:25	evaluation	16:25	evaluation	16:25	Preparation and evaluation of a novel chlorine dioxide preservative based on citric acid grafted carboxymethyl chitosan.					
37244340	0	50	theme	preservative	55:66	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and evaluation of a novel chlorine dioxide preservative based on citric acid grafted carboxymethyl chitosan.					
37244340	6	51	theme	microbial	1121:1129	arg1	counts					1138:1143	total microbial colony counts	1115:1143	total microbial colony counts	1115:1143	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	0	52	theme	chlorine	38:45	arg1	preservative					55:66	a novel chlorine dioxide preservative	30:66	a novel chlorine dioxide preservative based on citric acid	30:87	Preparation and evaluation of a novel chlorine dioxide preservative based on citric acid grafted carboxymethyl chitosan.					
37244340	6	53	theme	ClO2	859:862	arg1	time					872:875	The maximum ClO2 release time	847:875	The maximum ClO2 release time of this preservative	847:896	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	5	54	dep	composition	683:693	arg1	The					679:681	The	679:681	The	679:681	The composition and concentration of ClO2 slow-releasing preservative were optimized, and the best formulation was as follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1.					
37244340	7	55	theme	pulp	1378:1381	arg1	quality					1383:1389	pulp quality	1378:1389	pulp quality	1378:1389	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	5	56	dep	NaClO2	806:811	arg1	starch = 3:2:1:1					829:844	starch = 3:2:1:1	829:844	follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1	797:844	The composition and concentration of ClO2 slow-releasing preservative were optimized, and the best formulation was as follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1.					
37244340	5	56	dep	NaClO2	806:811	arg1	#					819:819	CMC-CA#2	813:820	follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1	797:844	The composition and concentration of ClO2 slow-releasing preservative were optimized, and the best formulation was as follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1.					
37244340	5	56	dep	NaClO2	806:811	arg1	Na2SO4					822:827	Na2SO4	822:827	follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1	797:844	The composition and concentration of ClO2 slow-releasing preservative were optimized, and the best formulation was as follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1.					
37244340	1	57	theme	Instrument-free	121:135	arg1	preservative					161:172	Instrument-free chlorine dioxide (ClO2) preservative	121:172	Instrument-free chlorine dioxide (ClO2) preservative for fruit and vegetable	121:196	Instrument-free chlorine dioxide (ClO2) preservative for fruit and vegetable has gained great attention due to its convenience and safety.					
37244340	6	58	theme	maximum	851:857	arg1	time					872:875	The maximum ClO2 release time	847:875	The maximum ClO2 release time of this preservative	847:896	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	7	59	theme	0.3 g	1234:1238	arg1	preservative					1245:1256	0.3 g ClO2 preservative	1234:1256	0.3 g ClO2 preservative	1234:1256	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	7	60	theme	highest	1266:1272	arg1	value					1277:1281	the highest L* value	1262:1281	the highest L* value of 47.47	1262:1290	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	1	61	theme	chlorine	137:144	arg1	preservative					161:172	Instrument-free chlorine dioxide (ClO2) preservative	121:172	Instrument-free chlorine dioxide (ClO2) preservative for fruit and vegetable	121:196	Instrument-free chlorine dioxide (ClO2) preservative for fruit and vegetable has gained great attention due to its convenience and safety.					
37244340	7	62	theme	L	1274:1274	arg1	value					1277:1281	the highest L* value	1262:1281	the highest L* value of 47.47	1262:1290	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	1	63	theme	due	225:227	arg1	attention					215:223	great attention	209:223	great attention due to its convenience and safety	209:257	Instrument-free chlorine dioxide (ClO2) preservative for fruit and vegetable has gained great attention due to its convenience and safety.					
37244340	5	64	dep	follows	797:803	arg1	NaClO2					806:811	NaClO2	806:811	follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1	797:844	The composition and concentration of ClO2 slow-releasing preservative were optimized, and the best formulation was as follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1.					
37244340	1	65	theme	dioxide	146:152	arg1	preservative					161:172	Instrument-free chlorine dioxide (ClO2) preservative	121:172	Instrument-free chlorine dioxide (ClO2) preservative for fruit and vegetable	121:196	Instrument-free chlorine dioxide (ClO2) preservative for fruit and vegetable has gained great attention due to its convenience and safety.					
37244340	6	66	theme	maximum	933:939	arg1	rate					949:952	the maximum release rate	929:952	the maximum release rate	929:952	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	6	67	theme	respiration	1094:1104	arg1	rate					1106:1109	lower respiration rate	1088:1109	lower respiration rate	1088:1109	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	3	68	theme	FT-IR	476:480	arg1	spectra					482:488	UV-Vis and FT-IR spectra	465:488	spectra	482:488	UV-Vis and FT-IR spectra revealed that CMC-CA#1-3 were successfully prepared.					
37244340	4	69	theme	mass	592:595	arg1	ratios					597:602	the mass ratios	588:602	the mass ratios of CA grafted in CMC-CA#1-3	588:630	Further potentiometric titration showed that the mass ratios of CA grafted in CMC-CA#1-3 were 0.18:1, 0.42:1, and 0.42:1, respectively.					
37244340	4	69	theme	mass	592:595	arg1	0.18:1					637:642	0.18:1	637:642	0.18:1	637:642	Further potentiometric titration showed that the mass ratios of CA grafted in CMC-CA#1-3 were 0.18:1, 0.42:1, and 0.42:1, respectively.					
37244340	2	70	theme	novel	414:418	arg1	preservative					440:451	a novel ClO2 slow-releasing preservative	412:451	a novel ClO2 slow-releasing preservative for longan	412:462	In this study, a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents were synthesized, characterized, and further used to prepare a novel ClO2 slow-releasing preservative for longan.					
37244340	7	71	theme	best	1354:1357	arg1	color					1368:1372	pericarp color	1359:1372	pericarp color	1359:1372	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
37244340	6	72	theme	lower	1088:1092	arg1	rate					1106:1109	lower respiration rate	1088:1109	lower respiration rate	1088:1109	The maximum ClO2 release time of this preservative reached >240 h at 5-25 °C, and the maximum release rate always occurred at 12-36 h. Longan treated with 0.15-1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative).					
37244340	1	73	theme	ClO2	155:158	arg1	preservative					161:172	Instrument-free chlorine dioxide (ClO2) preservative	121:172	Instrument-free chlorine dioxide (ClO2) preservative for fruit and vegetable	121:196	Instrument-free chlorine dioxide (ClO2) preservative for fruit and vegetable has gained great attention due to its convenience and safety.					
37244340	7	74	theme	*	1275:1275	arg1	value					1277:1281	the highest L* value	1262:1281	the highest L* value of 47.47	1262:1290	After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg-1·h-1, showing the best pericarp color and pulp quality.					
35882122	3	0	with	graft	478:482	arg1	structure					523:531	three-dimensional network structure	497:531	three-dimensional network structure	497:531	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure were constructed by electrospinning, which can mimic natural vascular biomechanics and enhance the viability of cells viability in vitro.					
35882122	7	1	theme	cells	1117:1121	arg1	proliferation					1067:1079	the proliferation	1063:1079	the proliferation of human umbilical vein endothelial cells (HUVECs)	1063:1130	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	2	2	theme	transplantation	400:414	arg1	rate					392:395	the success rate	380:395	the success rate of transplantation	380:414	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	8	3	theme	network	1195:1201	arg1	structure					1203:1211	the three-dimensional network structure	1173:1211	the three-dimensional network structure of PCLHC	1173:1220	In summary, all the results showed that the three-dimensional network structure of PCLHC presented the potential to heal injured vessels.					
35882122	7	4	theme	umbilical	1090:1098	arg1	HUVECs					1124:1129	HUVECs	1124:1129	HUVECs	1124:1129	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	7	4	theme	umbilical	1090:1098	arg1	cells					1117:1121	human umbilical vein endothelial cells	1084:1121	human umbilical vein endothelial cells (HUVECs)	1084:1130	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	7	5	theme	three-component	980:994	arg1	nanofibers					1003:1012	Simultaneous three-component hybrid nanofibers	967:1012	Simultaneous three-component hybrid nanofibers	967:1012	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	8	6	theme	injured	1254:1260	arg1	vessels					1262:1268	heal injured vessels	1249:1268	heal injured vessels	1249:1268	In summary, all the results showed that the three-dimensional network structure of PCLHC presented the potential to heal injured vessels.					
35882122	7	7	theme	vein	1100:1103	arg1	HUVECs					1124:1129	HUVECs	1124:1129	HUVECs	1124:1129	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	7	7	theme	vein	1100:1103	arg1	cells					1117:1121	human umbilical vein endothelial cells	1084:1121	human umbilical vein endothelial cells (HUVECs)	1084:1130	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	7	8	theme	hybrid	996:1001	arg1	nanofibers					1003:1012	Simultaneous three-component hybrid nanofibers	967:1012	Simultaneous three-component hybrid nanofibers	967:1012	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	5	9	contain	had	840:842	arg1	PCLHC					834:838	PCLHC	834:838	PCLHC	834:838	The results of mechanical experiments showed that PCLHC had similar mechanical properties to natural blood vessels.					
35882122	5	9	contain	had	840:842	arg2	properties					863:872	similar mechanical properties	844:872	similar mechanical properties	844:872	The results of mechanical experiments showed that PCLHC had similar mechanical properties to natural blood vessels.					
35882122	8	10	theme	heal	1249:1252	arg1	vessels					1262:1268	heal injured vessels	1249:1268	heal injured vessels	1249:1268	In summary, all the results showed that the three-dimensional network structure of PCLHC presented the potential to heal injured vessels.					
35882122	7	11	theme	human	1084:1088	arg1	HUVECs					1124:1129	HUVECs	1124:1129	HUVECs	1124:1129	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	7	11	theme	human	1084:1088	arg1	cells					1117:1121	human umbilical vein endothelial cells	1084:1121	human umbilical vein endothelial cells (HUVECs)	1084:1130	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	2	12	theme	aggregation	328:338	arg1	inhibition					305:314	inhibition	305:314	inhibition of platelet aggregation	305:338	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	2	12	theme	aggregation	328:338	arg1	vessels					293:299	autologous blood vessels	276:299	autologous blood vessels	276:299	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	7	13	theme	Simultaneous	967:978	arg1	nanofibers					1003:1012	Simultaneous three-component hybrid nanofibers	967:1012	Simultaneous three-component hybrid nanofibers	967:1012	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	1	14	theme	orthotopic	192:201	arg1	grafts					230:235	orthotopic tissue-engineered vascular grafts	192:235	orthotopic tissue-engineered vascular grafts	192:235	Inhibiting thrombus formation and intimal hyperplasia is essential for orthotopic tissue-engineered vascular grafts.					
35882122	5	15	theme	natural	877:883	arg1	vessels					891:897	natural blood vessels	877:897	natural blood vessels	877:897	The results of mechanical experiments showed that PCLHC had similar mechanical properties to natural blood vessels.					
35882122	0	16	dep	vascular	100:107	arg1	substitute					109:118	substitute	109:118	substitute	109:118	Electrospun PCL/collagen hybrid nanofibrous tubular graft based on post-network bond processing for vascular substitute.					
35882122	1	17	theme	tissue-engineered	203:219	arg1	grafts					230:235	orthotopic tissue-engineered vascular grafts	192:235	orthotopic tissue-engineered vascular grafts	192:235	Inhibiting thrombus formation and intimal hyperplasia is essential for orthotopic tissue-engineered vascular grafts.					
35882122	0	18	theme	PCL/collagen	12:23	arg1	graft					52:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft	0:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft	0:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft based on post-network bond processing for vascular substitute.					
35882122	8	19	theme	three-dimensional	1177:1193	arg1	structure					1203:1211	the three-dimensional network structure	1173:1211	the three-dimensional network structure of PCLHC	1173:1220	In summary, all the results showed that the three-dimensional network structure of PCLHC presented the potential to heal injured vessels.					
35882122	5	20	theme	similar	844:850	arg1	properties					863:872	similar mechanical properties	844:872	similar mechanical properties	844:872	The results of mechanical experiments showed that PCLHC had similar mechanical properties to natural blood vessels.					
35882122	1	21	theme	vascular	221:228	arg1	grafts					230:235	orthotopic tissue-engineered vascular grafts	192:235	orthotopic tissue-engineered vascular grafts	192:235	Inhibiting thrombus formation and intimal hyperplasia is essential for orthotopic tissue-engineered vascular grafts.					
35882122	0	22	theme	Electrospun	0:10	arg1	graft					52:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft	0:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft	0:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft based on post-network bond processing for vascular substitute.					
35882122	3	23	theme	cells	645:649	arg1	viability					632:640	the viability	628:640	the viability of cells	628:649	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure were constructed by electrospinning, which can mimic natural vascular biomechanics and enhance the viability of cells viability in vitro.					
35882122	4	24	theme	uniform	753:759	arg1	morphology					772:781	uniform and smooth morphology	753:781	uniform and smooth morphology	753:781	The hybrid collagen matrix network nanofibers formed by electrospinning exhibited uniform and smooth morphology.					
35882122	0	25	theme	nanofibrous	32:42	arg1	graft					52:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft	0:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft	0:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft based on post-network bond processing for vascular substitute.					
35882122	0	26	theme	hybrid	25:30	arg1	graft					52:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft	0:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft	0:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft based on post-network bond processing for vascular substitute.					
35882122	3	27	theme	vascular	594:601	arg1	biomechanics					603:614	natural vascular biomechanics	586:614	natural vascular biomechanics	586:614	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure were constructed by electrospinning, which can mimic natural vascular biomechanics and enhance the viability of cells viability in vitro.					
35882122	5	28	theme	experiments	810:820	arg1	results					788:794	The results	784:794	The results of mechanical experiments	784:820	The results of mechanical experiments showed that PCLHC had similar mechanical properties to natural blood vessels.					
35882122	4	29	theme	network	698:704	arg1	nanofibers					706:715	The hybrid collagen matrix network nanofibers	671:715	The hybrid collagen matrix network nanofibers formed by electrospinning	671:741	The hybrid collagen matrix network nanofibers formed by electrospinning exhibited uniform and smooth morphology.					
35882122	3	30	theme	vascular	469:476	arg1	PCLHC					485:489	PCLHC	485:489	PCLHC	485:489	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure were constructed by electrospinning, which can mimic natural vascular biomechanics and enhance the viability of cells viability in vitro.					
35882122	3	30	theme	vascular	469:476	arg1	graft					478:482	The poly(ε-caprolactone)/collagen/heparin composite vascular graft	417:482	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure	417:531	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure were constructed by electrospinning, which can mimic natural vascular biomechanics and enhance the viability of cells viability in vitro.					
35882122	5	31	theme	mechanical	799:808	arg1	experiments					810:820	mechanical experiments	799:820	mechanical experiments	799:820	The results of mechanical experiments showed that PCLHC had similar mechanical properties to natural blood vessels.					
35882122	2	32	theme	blood	287:291	arg1	vessels					293:299	autologous blood vessels	276:299	autologous blood vessels	276:299	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	3	33	theme	three-dimensional	497:513	arg1	structure					523:531	three-dimensional network structure	497:531	three-dimensional network structure	497:531	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure were constructed by electrospinning, which can mimic natural vascular biomechanics and enhance the viability of cells viability in vitro.					
35882122	0	34	theme	tubular	44:50	arg1	graft					52:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft	0:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft	0:56	Electrospun PCL/collagen hybrid nanofibrous tubular graft based on post-network bond processing for vascular substitute.					
35882122	5	35	theme	blood	885:889	arg1	vessels					891:897	natural blood vessels	877:897	natural blood vessels	877:897	The results of mechanical experiments showed that PCLHC had similar mechanical properties to natural blood vessels.					
35882122	6	36	theme	PCLHC	960:964	arg1	anticoagulation					941:955	the anticoagulation	937:955	the anticoagulation of PCLHC	937:964	And the addition of heparin enhanced the anticoagulation of PCLHC.					
35882122	2	37	theme	autologous	276:285	arg1	vessels					293:299	autologous blood vessels	276:299	autologous blood vessels	276:299	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	3	38	theme	network	515:521	arg1	structure					523:531	three-dimensional network structure	497:531	three-dimensional network structure	497:531	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure were constructed by electrospinning, which can mimic natural vascular biomechanics and enhance the viability of cells viability in vitro.					
35882122	3	39	theme	/collagen/heparin	441:457	arg1	PCLHC					485:489	PCLHC	485:489	PCLHC	485:489	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure were constructed by electrospinning, which can mimic natural vascular biomechanics and enhance the viability of cells viability in vitro.					
35882122	3	39	theme	/collagen/heparin	441:457	arg1	graft					478:482	The poly(ε-caprolactone)/collagen/heparin composite vascular graft	417:482	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure	417:531	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure were constructed by electrospinning, which can mimic natural vascular biomechanics and enhance the viability of cells viability in vitro.					
35882122	5	40	theme	mechanical	852:861	arg1	properties					863:872	similar mechanical properties	844:872	similar mechanical properties	844:872	The results of mechanical experiments showed that PCLHC had similar mechanical properties to natural blood vessels.					
35882122	1	41	theme	thrombus	132:139	arg1	formation					141:149	thrombus formation	132:149	thrombus formation	132:149	Inhibiting thrombus formation and intimal hyperplasia is essential for orthotopic tissue-engineered vascular grafts.					
35882122	3	42	theme	composite	459:467	arg1	PCLHC					485:489	PCLHC	485:489	PCLHC	485:489	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure were constructed by electrospinning, which can mimic natural vascular biomechanics and enhance the viability of cells viability in vitro.					
35882122	3	42	theme	composite	459:467	arg1	graft					478:482	The poly(ε-caprolactone)/collagen/heparin composite vascular graft	417:482	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure	417:531	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure were constructed by electrospinning, which can mimic natural vascular biomechanics and enhance the viability of cells viability in vitro.					
35882122	2	43	theme	mechanical	251:260	arg1	properties					262:271	The matching mechanical properties	238:271	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation	238:338	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	2	43	theme	mechanical	251:260	arg1	points					362:367	two points	358:367	two points	358:367	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	2	44	theme	success	384:390	arg1	rate					392:395	the success rate	380:395	the success rate of transplantation	380:414	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	7	45	theme	reliable	1035:1042	arg1	ability					1044:1050	a potentially reliable ability	1021:1050	a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs)	1021:1130	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	8	46	theme	PCLHC	1216:1220	arg1	structure					1203:1211	the three-dimensional network structure	1173:1211	the three-dimensional network structure of PCLHC	1173:1220	In summary, all the results showed that the three-dimensional network structure of PCLHC presented the potential to heal injured vessels.					
35882122	2	47	theme	matching	242:249	arg1	properties					262:271	The matching mechanical properties	238:271	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation	238:338	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	2	47	theme	matching	242:249	arg1	points					362:367	two points	358:367	two points	358:367	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	4	48	theme	smooth	765:770	arg1	morphology					772:781	uniform and smooth morphology	753:781	uniform and smooth morphology	753:781	The hybrid collagen matrix network nanofibers formed by electrospinning exhibited uniform and smooth morphology.					
35882122	4	49	theme	hybrid	675:680	arg1	nanofibers					706:715	The hybrid collagen matrix network nanofibers	671:715	The hybrid collagen matrix network nanofibers formed by electrospinning	671:741	The hybrid collagen matrix network nanofibers formed by electrospinning exhibited uniform and smooth morphology.					
35882122	2	50	theme	platelet	319:326	arg1	aggregation					328:338	platelet aggregation	319:338	platelet aggregation	319:338	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	2	51	theme	inhibition	305:314	arg1	properties					262:271	The matching mechanical properties	238:271	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation	238:338	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	2	51	theme	inhibition	305:314	arg1	points					362:367	two points	358:367	two points	358:367	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	1	52	theme	intimal	155:161	arg1	hyperplasia					163:173	intimal hyperplasia	155:173	intimal hyperplasia	155:173	Inhibiting thrombus formation and intimal hyperplasia is essential for orthotopic tissue-engineered vascular grafts.					
35882122	0	53	theme	bond	80:83	arg1	processing					85:94	post-network bond processing	67:94	post-network bond processing for vascular substitute	67:118	Electrospun PCL/collagen hybrid nanofibrous tubular graft based on post-network bond processing for vascular substitute.					
35882122	6	54	theme	heparin	920:926	arg1	addition					908:915	the addition	904:915	the addition of heparin	904:926	And the addition of heparin enhanced the anticoagulation of PCLHC.					
35882122	4	55	theme	matrix	691:696	arg1	nanofibers					706:715	The hybrid collagen matrix network nanofibers	671:715	The hybrid collagen matrix network nanofibers formed by electrospinning	671:741	The hybrid collagen matrix network nanofibers formed by electrospinning exhibited uniform and smooth morphology.					
35882122	0	56	theme	post-network	67:78	arg1	processing					85:94	post-network bond processing	67:94	post-network bond processing for vascular substitute	67:118	Electrospun PCL/collagen hybrid nanofibrous tubular graft based on post-network bond processing for vascular substitute.					
35882122	2	57	theme	vessels	293:299	arg1	properties					262:271	The matching mechanical properties	238:271	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation	238:338	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	2	57	theme	vessels	293:299	arg1	points					362:367	two points	358:367	two points	358:367	The matching mechanical properties of autologous blood vessels and inhibition of platelet aggregation are considered as two points to improve the success rate of transplantation.					
35882122	7	58	theme	endothelial	1105:1115	arg1	HUVECs					1124:1129	HUVECs	1124:1129	HUVECs	1124:1129	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	7	58	theme	endothelial	1105:1115	arg1	cells					1117:1121	human umbilical vein endothelial cells	1084:1121	human umbilical vein endothelial cells (HUVECs)	1084:1130	Simultaneous three-component hybrid nanofibers showed a potentially reliable ability to promote the proliferation of human umbilical vein endothelial cells (HUVECs).					
35882122	3	59	theme	natural	586:592	arg1	biomechanics					603:614	natural vascular biomechanics	586:614	natural vascular biomechanics	586:614	The poly(ε-caprolactone)/collagen/heparin composite vascular graft (PCLHC) with three-dimensional network structure were constructed by electrospinning, which can mimic natural vascular biomechanics and enhance the viability of cells viability in vitro.					
35882122	4	60	theme	collagen	682:689	arg1	nanofibers					706:715	The hybrid collagen matrix network nanofibers	671:715	The hybrid collagen matrix network nanofibers formed by electrospinning	671:741	The hybrid collagen matrix network nanofibers formed by electrospinning exhibited uniform and smooth morphology.					
35059938	12	0	theme	catalytic	1953:1961	arg1	performance					1963:1973	Improved catalytic performance	1944:1973	Improved catalytic performance	1944:1973	Improved catalytic performance suggests that multipoint covalent immobilization protocol adapted using hydrogel system could be tailored as microreactor for catalysis of profitable macromolecules.					
35059938	4	1	theme	immobilization	687:700	arg1	matrix					702:707	an immobilization matrix	684:707	an immobilization matrix	684:707	Hydrogel structure worked as an immobilization matrix and as a microreactor system to catalyze the cleavage of a disaccharide.					
35059938	3	2	theme	hydrogel	592:599	arg1	system					601:606	chitosan-based hydrogel system	577:606	chitosan-based hydrogel system crosslinked together with glucosyltransferase	577:652	RESULTS Present study aims towards development of chitosan-based hydrogel system crosslinked together with glucosyltransferase.					
35059938	10	3	with	15.23 kJ mol-1	1700:1713	arg1	values					1766:1771	increased Vmax (785 DSU ml-1) and Km (256 mM) values	1720:1771	increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version	1720:1809	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	10	3	with	15.23 kJ mol-1	1700:1713	arg1	256 mM					1758:1763	256 mM	1758:1763	256 mM	1758:1763	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	10	4	theme	DSU	1740:1742	arg1	Vmax					1730:1733	increased Vmax	1720:1733	increased Vmax (785 DSU ml-1)	1720:1748	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	10	4	theme	DSU	1740:1742	arg1	ml-1					1744:1747	785 DSU ml-1	1736:1747	785 DSU ml-1	1736:1747	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	11	5	theme	Thermal	1812:1818	arg1	stability					1820:1828	Thermal stability	1812:1828	Thermal stability of the crosslinked dextransucrase	1812:1862	Thermal stability of the crosslinked dextransucrase also particularly improved 2.5 fold at 45 °C in comparison with soluble enzyme.					
35059938	10	6	dep	values	1766:1771	arg1	compared					1776:1783	compared	1776:1783	compared to soluble enzyme version	1776:1809	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	2	7	contain	have	314:317	arg1	Biomacromolecules					290:306	Biomacromolecules	290:306	Biomacromolecules which have reactive functional groups in their structural frame and can also exhibit hydrogel properties	290:411	Biomacromolecules which have reactive functional groups in their structural frame and can also exhibit hydrogel properties could be utilized in biomedical, pharmaceutical and drug delivery applications after some chemical modifications.					
35059938	2	7	contain	have	314:317	arg2	groups					339:344	reactive functional groups	319:344	reactive functional groups	319:344	Biomacromolecules which have reactive functional groups in their structural frame and can also exhibit hydrogel properties could be utilized in biomedical, pharmaceutical and drug delivery applications after some chemical modifications.					
35059938	2	8	theme	reactive	319:326	arg1	groups					339:344	reactive functional groups	319:344	reactive functional groups	319:344	Biomacromolecules which have reactive functional groups in their structural frame and can also exhibit hydrogel properties could be utilized in biomedical, pharmaceutical and drug delivery applications after some chemical modifications.					
35059938	1	9	theme	polymer	199:205	arg1	composition					207:217	backbone polymer composition	190:217	backbone polymer composition	190:217	OBJECTIVES Diversity in backbone polymer composition makes hydrogel-based resources open to broad spectrum of applications.					
35059938	10	10	theme	Km	1754:1755	arg1	values					1766:1771	increased Vmax (785 DSU ml-1) and Km (256 mM) values	1720:1771	increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version	1720:1809	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	10	10	theme	Km	1754:1755	arg1	256 mM					1758:1763	256 mM	1758:1763	256 mM	1758:1763	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	9	11	theme	Enzyme	1383:1388	arg1	fragments					1390:1398	Enzyme fragments	1383:1398	Enzyme fragments	1383:1398	Enzyme fragments were observed bound with hydrogel beads when screened for surface topology indicating successful crosslinking.					
35059938	8	12	theme	available	1367:1375	arg1	ends					1377:1380	all available ends	1363:1380	all available ends	1363:1380	Hydrogel beads required 18.0 h crosslinking time with enzyme (6.5 mg ml-1, 189.9 DSU) under specific environment (4 °C, 100 rpm) to saturate all available ends.					
35059938	0	13	theme	multipoint	124:133	arg1	approach					156:163	multipoint covalent interaction approach	124:163	multipoint covalent interaction approach	124:163	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.					
35059938	7	14	with	functionalization	1114:1130	arg1	fragments					1211:1219	enzyme fragments	1204:1219	enzyme fragments	1204:1219	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	0	15	theme	interaction	144:154	arg1	approach					156:163	multipoint covalent interaction approach	124:163	multipoint covalent interaction approach	124:163	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.					
35059938	11	16	with	comparison	1912:1921	arg1	enzyme					1936:1941	soluble enzyme	1928:1941	soluble enzyme	1928:1941	Thermal stability of the crosslinked dextransucrase also particularly improved 2.5 fold at 45 °C in comparison with soluble enzyme.					
35059938	5	17	theme	chitosan	790:797	arg1	beads					808:812	Uniform chitosan hydrogel beads	782:812	Uniform chitosan hydrogel beads	782:812	Uniform chitosan hydrogel beads were prepared and dextransucrase was attached using multipoint covalent interaction approach.					
35059938	9	18	theme	hydrogel	1425:1432	arg1	beads					1434:1438	hydrogel beads	1425:1438	hydrogel beads	1425:1438	Enzyme fragments were observed bound with hydrogel beads when screened for surface topology indicating successful crosslinking.					
35059938	7	19	theme	matrix	1185:1190	arg1	system					1192:1197	this activated matrix system	1170:1197	this activated matrix system	1170:1197	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	5	20	theme	interaction	886:896	arg1	approach					898:905	multipoint covalent interaction approach	866:905	multipoint covalent interaction approach	866:905	Uniform chitosan hydrogel beads were prepared and dextransucrase was attached using multipoint covalent interaction approach.					
35059938	1	21	theme	OBJECTIVES	166:175	arg1	Diversity					177:185	OBJECTIVES Diversity	166:185	OBJECTIVES Diversity in backbone polymer composition	166:217	OBJECTIVES Diversity in backbone polymer composition makes hydrogel-based resources open to broad spectrum of applications.					
35059938	11	22	theme	soluble	1928:1934	arg1	enzyme					1936:1941	soluble enzyme	1928:1941	soluble enzyme	1928:1941	Thermal stability of the crosslinked dextransucrase also particularly improved 2.5 fold at 45 °C in comparison with soluble enzyme.					
35059938	1	23	from	Diversity	177:185	arg1	composition					207:217	backbone polymer composition	190:217	backbone polymer composition	190:217	OBJECTIVES Diversity in backbone polymer composition makes hydrogel-based resources open to broad spectrum of applications.					
35059938	0	24	from	Exploration	0:10	arg1	form					66:69	the form	62:69	the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach	62:163	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.					
35059938	0	25	theme	polyaminosaccharide	83:101	arg1	beads					112:116	reactive polyaminosaccharide hydrogel beads	74:116	reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach	74:163	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.					
35059938	9	26	theme	successful	1486:1495	arg1	crosslinking					1497:1508	successful crosslinking	1486:1508	successful crosslinking	1486:1508	Enzyme fragments were observed bound with hydrogel beads when screened for surface topology indicating successful crosslinking.					
35059938	8	27	theme	Hydrogel	1222:1229	arg1	beads					1231:1235	Hydrogel beads	1222:1235	Hydrogel beads	1222:1235	Hydrogel beads required 18.0 h crosslinking time with enzyme (6.5 mg ml-1, 189.9 DSU) under specific environment (4 °C, 100 rpm) to saturate all available ends.					
35059938	2	28	used	utilized	422:429	arg2	Biomacromolecules					290:306	Biomacromolecules	290:306	Biomacromolecules which have reactive functional groups in their structural frame and can also exhibit hydrogel properties	290:411	Biomacromolecules which have reactive functional groups in their structural frame and can also exhibit hydrogel properties could be utilized in biomedical, pharmaceutical and drug delivery applications after some chemical modifications.					
35059938	10	29	theme	enzyme	1796:1801	arg1	version					1803:1809	soluble enzyme version	1788:1809	soluble enzyme version	1788:1809	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	5	30	theme	multipoint	866:875	arg1	approach					898:905	multipoint covalent interaction approach	866:905	multipoint covalent interaction approach	866:905	Uniform chitosan hydrogel beads were prepared and dextransucrase was attached using multipoint covalent interaction approach.					
35059938	0	31	from	matrix	35:40	arg1	form					66:69	the form	62:69	the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach	62:163	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.					
35059938	8	32	theme	crosslinking	1253:1264	arg1	time					1266:1269	18.0 h crosslinking time	1246:1269	18.0 h crosslinking time with enzyme (6.5 mg ml-1, 189.9 DSU) under specific environment (4 °C, 100 rpm)	1246:1349	Hydrogel beads required 18.0 h crosslinking time with enzyme (6.5 mg ml-1, 189.9 DSU) under specific environment (4 °C, 100 rpm) to saturate all available ends.					
35059938	10	33	with	35 °C	1649:1653	arg1	energy					1672:1677	an energy	1669:1677	an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version	1669:1809	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	4	34	theme	Hydrogel	655:662	arg1	structure					664:672	Hydrogel structure	655:672	Hydrogel structure	655:672	Hydrogel structure worked as an immobilization matrix and as a microreactor system to catalyze the cleavage of a disaccharide.					
35059938	3	35	theme	Present	535:541	arg1	study					543:547	RESULTS Present study	527:547	RESULTS Present study	527:547	RESULTS Present study aims towards development of chitosan-based hydrogel system crosslinked together with glucosyltransferase.					
35059938	12	36	theme	hydrogel	2047:2054	arg1	system					2056:2061	hydrogel system	2047:2061	hydrogel system	2047:2061	Improved catalytic performance suggests that multipoint covalent immobilization protocol adapted using hydrogel system could be tailored as microreactor for catalysis of profitable macromolecules.					
35059938	11	37	theme	dextransucrase	1849:1862	arg1	stability					1820:1828	Thermal stability	1812:1828	Thermal stability of the crosslinked dextransucrase	1812:1862	Thermal stability of the crosslinked dextransucrase also particularly improved 2.5 fold at 45 °C in comparison with soluble enzyme.					
35059938	2	38	theme	drug	465:468	arg1	delivery					470:477	drug delivery	465:477	drug delivery	465:477	Biomacromolecules which have reactive functional groups in their structural frame and can also exhibit hydrogel properties could be utilized in biomedical, pharmaceutical and drug delivery applications after some chemical modifications.					
35059938	0	39	theme	matrix	35:40	arg1	Exploration					0:10	Exploration	0:10	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.	0:164	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.					
35059938	2	40	theme	hydrogel	393:400	arg1	properties					402:411	hydrogel properties	393:411	hydrogel properties	393:411	Biomacromolecules which have reactive functional groups in their structural frame and can also exhibit hydrogel properties could be utilized in biomedical, pharmaceutical and drug delivery applications after some chemical modifications.					
35059938	12	41	theme	immobilization	2009:2022	arg1	microreactor					2084:2095	microreactor	2084:2095	microreactor	2084:2095	Improved catalytic performance suggests that multipoint covalent immobilization protocol adapted using hydrogel system could be tailored as microreactor for catalysis of profitable macromolecules.					
35059938	12	41	theme	immobilization	2009:2022	arg1	protocol					2024:2031	multipoint covalent immobilization protocol	1989:2031	multipoint covalent immobilization protocol adapted using hydrogel system	1989:2061	Improved catalytic performance suggests that multipoint covalent immobilization protocol adapted using hydrogel system could be tailored as microreactor for catalysis of profitable macromolecules.					
35059938	12	42	theme	covalent	2000:2007	arg1	microreactor					2084:2095	microreactor	2084:2095	microreactor	2084:2095	Improved catalytic performance suggests that multipoint covalent immobilization protocol adapted using hydrogel system could be tailored as microreactor for catalysis of profitable macromolecules.					
35059938	12	42	theme	covalent	2000:2007	arg1	protocol					2024:2031	multipoint covalent immobilization protocol	1989:2031	multipoint covalent immobilization protocol adapted using hydrogel system	1989:2061	Improved catalytic performance suggests that multipoint covalent immobilization protocol adapted using hydrogel system could be tailored as microreactor for catalysis of profitable macromolecules.					
35059938	2	43	dep	biomedical	434:443	arg1	applications					479:490	applications	479:490	applications	479:490	Biomacromolecules which have reactive functional groups in their structural frame and can also exhibit hydrogel properties could be utilized in biomedical, pharmaceutical and drug delivery applications after some chemical modifications.					
35059938	10	44	theme	state	1518:1522	arg1	kinetics					1524:1531	Steady state kinetics	1511:1531	Steady state kinetics of crosslinked dextransucrase	1511:1561	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	7	45	theme	important	1084:1092	arg1	tasks					1094:1098	two important tasks	1080:1098	two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments	1080:1219	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	1	46	theme	broad	258:262	arg1	spectrum					264:271	broad spectrum	258:271	broad spectrum of applications	258:287	OBJECTIVES Diversity in backbone polymer composition makes hydrogel-based resources open to broad spectrum of applications.					
35059938	12	47	theme	Improved	1944:1951	arg1	performance					1963:1973	Improved catalytic performance	1944:1973	Improved catalytic performance	1944:1973	Improved catalytic performance suggests that multipoint covalent immobilization protocol adapted using hydrogel system could be tailored as microreactor for catalysis of profitable macromolecules.					
35059938	10	48	theme	Vmax	1730:1733	arg1	values					1766:1771	increased Vmax (785 DSU ml-1) and Km (256 mM) values	1720:1771	increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version	1720:1809	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	10	48	theme	Vmax	1730:1733	arg1	256 mM					1758:1763	256 mM	1758:1763	256 mM	1758:1763	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	3	49	theme	RESULTS	527:533	arg1	study					543:547	RESULTS Present study	527:547	RESULTS Present study	527:547	RESULTS Present study aims towards development of chitosan-based hydrogel system crosslinked together with glucosyltransferase.					
35059938	3	50	theme	system	601:606	arg1	development					562:572	development	562:572	development of chitosan-based hydrogel system crosslinked together with glucosyltransferase	562:652	RESULTS Present study aims towards development of chitosan-based hydrogel system crosslinked together with glucosyltransferase.					
35059938	4	51	theme	microreactor	718:729	arg1	system					731:736	a microreactor system	716:736	a microreactor system to catalyze the cleavage of a disaccharide	716:779	Hydrogel structure worked as an immobilization matrix and as a microreactor system to catalyze the cleavage of a disaccharide.					
35059938	10	52	link	crosslinked	1536:1546	arg1	dextransucrase					1548:1561	crosslinked dextransucrase	1536:1561	crosslinked dextransucrase	1536:1561	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	10	53	theme	dextransucrase	1548:1561	arg1	kinetics					1524:1531	Steady state kinetics	1511:1531	Steady state kinetics of crosslinked dextransucrase	1511:1561	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	3	54	theme	chitosan-based	577:590	arg1	system					601:606	chitosan-based hydrogel system	577:606	chitosan-based hydrogel system crosslinked together with glucosyltransferase	577:652	RESULTS Present study aims towards development of chitosan-based hydrogel system crosslinked together with glucosyltransferase.					
35059938	7	55	theme	crosslinking	1051:1062	arg1	agent					1064:1068	This bifunctional crosslinking agent	1033:1068	This bifunctional crosslinking agent	1033:1068	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	11	56	link	crosslinked	1837:1847	arg1	dextransucrase					1849:1862	the crosslinked dextransucrase	1833:1862	the crosslinked dextransucrase	1833:1862	Thermal stability of the crosslinked dextransucrase also particularly improved 2.5 fold at 45 °C in comparison with soluble enzyme.					
35059938	6	57	theme	polymeric	952:960	arg1	hydrogel					962:969	polymeric hydrogel	952:969	polymeric hydrogel	952:969	Strong interaction was developed by linking polymeric hydrogel with the biocatalyst utilizing glutaraldehyde as spacer arms.					
35059938	2	58	theme	functional	328:337	arg1	groups					339:344	reactive functional groups	319:344	reactive functional groups	319:344	Biomacromolecules which have reactive functional groups in their structural frame and can also exhibit hydrogel properties could be utilized in biomedical, pharmaceutical and drug delivery applications after some chemical modifications.					
35059938	1	59	theme	backbone	190:197	arg1	composition					207:217	backbone polymer composition	190:217	backbone polymer composition	190:217	OBJECTIVES Diversity in backbone polymer composition makes hydrogel-based resources open to broad spectrum of applications.					
35059938	10	60	from	sucrose	1626:1632	arg1	30.0 min					1637:1644	30.0 min	1637:1644	30.0 min	1637:1644	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	8	61	with	time	1266:1269	arg1	enzyme					1276:1281	enzyme	1276:1281	enzyme (6.5 mg ml-1, 189.9 DSU)	1276:1306	Hydrogel beads required 18.0 h crosslinking time with enzyme (6.5 mg ml-1, 189.9 DSU) under specific environment (4 °C, 100 rpm) to saturate all available ends.					
35059938	4	62	theme	disaccharide	768:779	arg1	cleavage					754:761	the cleavage	750:761	the cleavage of a disaccharide	750:779	Hydrogel structure worked as an immobilization matrix and as a microreactor system to catalyze the cleavage of a disaccharide.					
35059938	0	63	theme	covalent	135:142	arg1	approach					156:163	multipoint covalent interaction approach	124:163	multipoint covalent interaction approach	124:163	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.					
35059938	6	64	theme	Strong	908:913	arg1	interaction					915:925	Strong interaction	908:925	Strong interaction	908:925	Strong interaction was developed by linking polymeric hydrogel with the biocatalyst utilizing glutaraldehyde as spacer arms.					
35059938	2	65	theme	structural	355:364	arg1	frame					366:370	their structural frame	349:370	their structural frame	349:370	Biomacromolecules which have reactive functional groups in their structural frame and can also exhibit hydrogel properties could be utilized in biomedical, pharmaceutical and drug delivery applications after some chemical modifications.					
35059938	7	66	theme	beads	1144:1148	arg1	crosslinking					1154:1165	crosslinking	1154:1165	crosslinking of this activated matrix system with enzyme fragments	1154:1219	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	7	66	theme	beads	1144:1148	arg1	functionalization					1114:1130	functionalization	1114:1130	functionalization of hydrogel beads	1114:1148	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	1	67	theme	hydrogel-based	225:238	arg1	resources					240:248	hydrogel-based resources	225:248	hydrogel-based resources open to broad spectrum of applications	225:287	OBJECTIVES Diversity in backbone polymer composition makes hydrogel-based resources open to broad spectrum of applications.					
35059938	1	68	theme	open	250:253	arg1	resources					240:248	hydrogel-based resources	225:248	hydrogel-based resources open to broad spectrum of applications	225:287	OBJECTIVES Diversity in backbone polymer composition makes hydrogel-based resources open to broad spectrum of applications.					
35059938	0	69	from	form	66:69	arg1	Exploration					0:10	Exploration	0:10	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.	0:164	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.					
35059938	8	70	dep	enzyme	1276:1281	arg1	6.5 mg ml-1					1284:1294	6.5 mg ml-1	1284:1294	6.5 mg ml-1	1284:1294	Hydrogel beads required 18.0 h crosslinking time with enzyme (6.5 mg ml-1, 189.9 DSU) under specific environment (4 °C, 100 rpm) to saturate all available ends.					
35059938	8	70	dep	enzyme	1276:1281	arg1	DSU					1303:1305	189.9 DSU	1297:1305	189.9 DSU	1297:1305	Hydrogel beads required 18.0 h crosslinking time with enzyme (6.5 mg ml-1, 189.9 DSU) under specific environment (4 °C, 100 rpm) to saturate all available ends.					
35059938	5	71	theme	Uniform	782:788	arg1	beads					808:812	Uniform chitosan hydrogel beads	782:812	Uniform chitosan hydrogel beads	782:812	Uniform chitosan hydrogel beads were prepared and dextransucrase was attached using multipoint covalent interaction approach.					
35059938	7	72	theme	enzyme	1204:1209	arg1	fragments					1211:1219	enzyme fragments	1204:1219	enzyme fragments	1204:1219	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	5	73	theme	hydrogel	799:806	arg1	beads					808:812	Uniform chitosan hydrogel beads	782:812	Uniform chitosan hydrogel beads	782:812	Uniform chitosan hydrogel beads were prepared and dextransucrase was attached using multipoint covalent interaction approach.					
35059938	7	74	theme	system	1192:1197	arg1	crosslinking					1154:1165	crosslinking	1154:1165	crosslinking of this activated matrix system with enzyme fragments	1154:1219	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	7	74	theme	system	1192:1197	arg1	functionalization					1114:1130	functionalization	1114:1130	functionalization of hydrogel beads	1114:1148	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	7	75	with	crosslinking	1154:1165	arg1	fragments					1211:1219	enzyme fragments	1204:1219	enzyme fragments	1204:1219	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	5	76	theme	covalent	877:884	arg1	approach					898:905	multipoint covalent interaction approach	866:905	multipoint covalent interaction approach	866:905	Uniform chitosan hydrogel beads were prepared and dextransucrase was attached using multipoint covalent interaction approach.					
35059938	7	77	theme	activated	1175:1183	arg1	system					1192:1197	this activated matrix system	1170:1197	this activated matrix system	1170:1197	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	0	78	theme	reactive	74:81	arg1	beads					112:116	reactive polyaminosaccharide hydrogel beads	74:116	reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach	74:163	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.					
35059938	9	79	theme	surface	1458:1464	arg1	topology					1466:1473	surface topology	1458:1473	surface topology indicating successful crosslinking	1458:1508	Enzyme fragments were observed bound with hydrogel beads when screened for surface topology indicating successful crosslinking.					
35059938	12	80	theme	profitable	2114:2123	arg1	macromolecules					2125:2138	profitable macromolecules	2114:2138	profitable macromolecules	2114:2138	Improved catalytic performance suggests that multipoint covalent immobilization protocol adapted using hydrogel system could be tailored as microreactor for catalysis of profitable macromolecules.					
35059938	0	81	theme	hydrogel	103:110	arg1	beads					112:116	reactive polyaminosaccharide hydrogel beads	74:116	reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach	74:163	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.					
35059938	12	82	theme	macromolecules	2125:2138	arg1	catalysis					2101:2109	catalysis	2101:2109	catalysis of profitable macromolecules	2101:2138	Improved catalytic performance suggests that multipoint covalent immobilization protocol adapted using hydrogel system could be tailored as microreactor for catalysis of profitable macromolecules.					
35059938	8	83	theme	18.0 h	1246:1251	arg1	time					1266:1269	18.0 h crosslinking time	1246:1269	18.0 h crosslinking time with enzyme (6.5 mg ml-1, 189.9 DSU) under specific environment (4 °C, 100 rpm)	1246:1349	Hydrogel beads required 18.0 h crosslinking time with enzyme (6.5 mg ml-1, 189.9 DSU) under specific environment (4 °C, 100 rpm) to saturate all available ends.					
35059938	7	84	theme	hydrogel	1135:1142	arg1	beads					1144:1148	hydrogel beads	1135:1148	hydrogel beads	1135:1148	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	8	85	dep	environment	1323:1333	arg1	100 rpm					1342:1348	100 rpm	1342:1348	100 rpm	1342:1348	Hydrogel beads required 18.0 h crosslinking time with enzyme (6.5 mg ml-1, 189.9 DSU) under specific environment (4 °C, 100 rpm) to saturate all available ends.					
35059938	8	85	dep	environment	1323:1333	arg1	4 °C					1336:1339	4 °C	1336:1339	4 °C	1336:1339	Hydrogel beads required 18.0 h crosslinking time with enzyme (6.5 mg ml-1, 189.9 DSU) under specific environment (4 °C, 100 rpm) to saturate all available ends.					
35059938	10	86	theme	soluble	1788:1794	arg1	version					1803:1809	soluble enzyme version	1788:1809	soluble enzyme version	1788:1809	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	2	87	theme	chemical	503:510	arg1	modifications					512:524	some chemical modifications	498:524	some chemical modifications	498:524	Biomacromolecules which have reactive functional groups in their structural frame and can also exhibit hydrogel properties could be utilized in biomedical, pharmaceutical and drug delivery applications after some chemical modifications.					
35059938	10	88	theme	Steady	1511:1516	arg1	kinetics					1524:1531	Steady state kinetics	1511:1531	Steady state kinetics of crosslinked dextransucrase	1511:1561	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	0	89	theme	three-dimensional	17:33	arg1	matrix					35:40	a three-dimensional matrix	15:40	a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach	15:163	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.					
35059938	11	90	theme	crosslinked	1837:1847	arg1	dextransucrase					1849:1862	the crosslinked dextransucrase	1833:1862	the crosslinked dextransucrase	1833:1862	Thermal stability of the crosslinked dextransucrase also particularly improved 2.5 fold at 45 °C in comparison with soluble enzyme.					
35059938	6	91	theme	spacer	1020:1025	arg1	glutaraldehyde					1002:1015	glutaraldehyde	1002:1015	glutaraldehyde	1002:1015	Strong interaction was developed by linking polymeric hydrogel with the biocatalyst utilizing glutaraldehyde as spacer arms.					
35059938	6	91	theme	spacer	1020:1025	arg1	arms					1027:1030	spacer arms	1020:1030	spacer arms	1020:1030	Strong interaction was developed by linking polymeric hydrogel with the biocatalyst utilizing glutaraldehyde as spacer arms.					
35059938	10	92	theme	crosslinked	1536:1546	arg1	dextransucrase					1548:1561	crosslinked dextransucrase	1536:1561	crosslinked dextransucrase	1536:1561	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	0	93	theme	beads	112:116	arg1	form					66:69	the form	62:69	the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach	62:163	Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach.					
35059938	10	94	theme	activation	1682:1691	arg1	energy					1672:1677	an energy	1669:1677	an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version	1669:1809	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	12	95	theme	multipoint	1989:1998	arg1	microreactor					2084:2095	microreactor	2084:2095	microreactor	2084:2095	Improved catalytic performance suggests that multipoint covalent immobilization protocol adapted using hydrogel system could be tailored as microreactor for catalysis of profitable macromolecules.					
35059938	12	95	theme	multipoint	1989:1998	arg1	protocol					2024:2031	multipoint covalent immobilization protocol	1989:2031	multipoint covalent immobilization protocol adapted using hydrogel system	1989:2061	Improved catalytic performance suggests that multipoint covalent immobilization protocol adapted using hydrogel system could be tailored as microreactor for catalysis of profitable macromolecules.					
35059938	8	96	theme	specific	1314:1321	arg1	environment					1323:1333	specific environment	1314:1333	specific environment (4 °C, 100 rpm)	1314:1349	Hydrogel beads required 18.0 h crosslinking time with enzyme (6.5 mg ml-1, 189.9 DSU) under specific environment (4 °C, 100 rpm) to saturate all available ends.					
35059938	10	97	theme	increased	1720:1728	arg1	Vmax					1730:1733	increased Vmax	1720:1733	increased Vmax (785 DSU ml-1)	1720:1748	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	10	97	theme	increased	1720:1728	arg1	ml-1					1744:1747	785 DSU ml-1	1736:1747	785 DSU ml-1	1736:1747	Steady state kinetics of crosslinked dextransucrase was studied in detail and it was revealed that it can catalyse sucrose in 30.0 min at 35 °C (pH 5.5) with an energy of activation around 15.23 kJ mol-1 with increased Vmax (785 DSU ml-1) and Km (256 mM) values as compared to soluble enzyme version.					
35059938	7	98	theme	bifunctional	1038:1049	arg1	agent					1064:1068	This bifunctional crosslinking agent	1033:1068	This bifunctional crosslinking agent	1033:1068	This bifunctional crosslinking agent performed two important tasks that includes functionalization of hydrogel beads and crosslinking of this activated matrix system with enzyme fragments.					
35059938	1	99	theme	applications	276:287	arg1	spectrum					264:271	broad spectrum	258:271	broad spectrum of applications	258:287	OBJECTIVES Diversity in backbone polymer composition makes hydrogel-based resources open to broad spectrum of applications.					
37105247	3	0	theme	River	834:838	arg1	water					840:844	Yangtze River water	826:844	Yangtze River water	826:844	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	0	1	theme	ultra-efficient	94:108	arg1	removal					110:116	pH-universal ultra-efficient removal	81:116	pH-universal ultra-efficient removal of dye	81:123	Chitosan-based composite hydrogel with a rigid-in-flexible network structure for pH-universal ultra-efficient removal of dye.					
37105247	1	2	theme	high	199:202	arg1	capacity					215:222	high adsorption capacity	199:222	high adsorption capacity	199:222	Polysaccharide-based hydrogel adsorbents become popular because of their high adsorption capacity and fast adsorption rate, but their low removal rate and poor pH resistance have always been fatal shortcomings.					
37105247	1	3	theme	poor	281:284	arg1	resistance					289:298	poor pH resistance	281:298	poor pH resistance	281:298	Polysaccharide-based hydrogel adsorbents become popular because of their high adsorption capacity and fast adsorption rate, but their low removal rate and poor pH resistance have always been fatal shortcomings.					
37105247	0	4	theme	dye	121:123	arg1	removal					110:116	pH-universal ultra-efficient removal	81:116	pH-universal ultra-efficient removal of dye	81:123	Chitosan-based composite hydrogel with a rigid-in-flexible network structure for pH-universal ultra-efficient removal of dye.					
37105247	3	5	theme	real	1003:1006	arg1	waters					1008:1013	real waters	1003:1013	real waters	1003:1013	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	1	6	theme	pH	286:287	arg1	resistance					289:298	poor pH resistance	281:298	poor pH resistance	281:298	Polysaccharide-based hydrogel adsorbents become popular because of their high adsorption capacity and fast adsorption rate, but their low removal rate and poor pH resistance have always been fatal shortcomings.					
37105247	3	7	from	MB	997:998	arg1	waters					1008:1013	real waters	1003:1013	real waters	1003:1013	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	8	theme	removal	972:978	arg1	rate					980:983	the removal rate	968:983	the removal rate (99.6 % for MB in real waters)	968:1014	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	9	theme	Yangtze	826:832	arg1	water					840:844	Yangtze River water	826:844	Yangtze River water	826:844	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	2	10	theme	chitosan-g-poly	513:527	arg1	network					584:590	chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network	513:590	chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure	513:639	Herein, a feasible strategy was proposed to strengthen the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure.					
37105247	3	11	theme	higher	914:919	arg1	content					921:927	higher content	914:927	higher content of REC (15.8 wt% REC)	914:949	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	1	12	theme	adsorption	204:213	arg1	capacity					215:222	high adsorption capacity	199:222	high adsorption capacity	199:222	Polysaccharide-based hydrogel adsorbents become popular because of their high adsorption capacity and fast adsorption rate, but their low removal rate and poor pH resistance have always been fatal shortcomings.					
37105247	3	13	theme	adsorption	736:745	arg1	capacities					747:756	its adsorption capacities	732:756	its adsorption capacities	732:756	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	2	14	theme	natural	484:490	arg1	REC					503:505	REC	503:505	REC	503:505	Herein, a feasible strategy was proposed to strengthen the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure.					
37105247	2	14	theme	natural	484:490	arg1	rectorite					492:500	natural rectorite	484:500	natural rectorite (REC)	484:506	Herein, a feasible strategy was proposed to strengthen the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure.					
37105247	1	15	dep	adsorbents	156:165	arg1	become					167:172	become	167:172	adsorbents become popular because of their high adsorption capacity and fast adsorption rate	156:247	Polysaccharide-based hydrogel adsorbents become popular because of their high adsorption capacity and fast adsorption rate, but their low removal rate and poor pH resistance have always been fatal shortcomings.					
37105247	2	16	theme	hydrogel	407:414	arg1	ability					396:402	the ability	392:402	the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure	392:639	Herein, a feasible strategy was proposed to strengthen the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure.					
37105247	0	17	theme	composite	15:23	arg1	hydrogel					25:32	Chitosan-based composite hydrogel	0:32	Chitosan-based composite hydrogel with a rigid-in-flexible network structure for pH-universal ultra-efficient removal of dye.	0:124	Chitosan-based composite hydrogel with a rigid-in-flexible network structure for pH-universal ultra-efficient removal of dye.					
37105247	2	18	dep	dye	462:464	arg1	i.e.					456:459	i.e.	456:459	i.e.	456:459	Herein, a feasible strategy was proposed to strengthen the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure.					
37105247	0	19	theme	Chitosan-based	0:13	arg1	hydrogel					25:32	Chitosan-based composite hydrogel	0:32	Chitosan-based composite hydrogel with a rigid-in-flexible network structure for pH-universal ultra-efficient removal of dye.	0:124	Chitosan-based composite hydrogel with a rigid-in-flexible network structure for pH-universal ultra-efficient removal of dye.					
37105247	3	20	theme	REC	932:934	arg1	content					921:927	higher content	914:927	higher content of REC (15.8 wt% REC)	914:949	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	1	21	theme	fast	228:231	arg1	rate					244:247	fast adsorption rate	228:247	fast adsorption rate	228:247	Polysaccharide-based hydrogel adsorbents become popular because of their high adsorption capacity and fast adsorption rate, but their low removal rate and poor pH resistance have always been fatal shortcomings.					
37105247	2	22	dep	chitosan-g-poly	513:527	arg1	2-acrylamido-2-methyl-propane-sulfonic-acid					530:572	2-acrylamido-2-methyl-propane-sulfonic-acid	530:572	2-acrylamido-2-methyl-propane-sulfonic-acid	530:572	Herein, a feasible strategy was proposed to strengthen the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure.					
37105247	1	23	theme	adsorption	233:242	arg1	rate					244:247	fast adsorption rate	228:247	fast adsorption rate	228:247	Polysaccharide-based hydrogel adsorbents become popular because of their high adsorption capacity and fast adsorption rate, but their low removal rate and poor pH resistance have always been fatal shortcomings.					
37105247	3	24	theme	blue	775:778	arg1	MB					781:782	MB	781:782	MB	781:782	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	24	theme	blue	775:778	arg1	methylene					765:773	methylene blue	765:778	methylene blue (MB)	765:783	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	25	theme	1.2 wt	682:687	arg1	REC					677:679	REC	677:679	REC (1.2 wt%)	677:689	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	25	theme	1.2 wt	682:687	arg1	%					688:688	1.2 wt%	682:688	1.2 wt%	682:688	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	1	26	theme	Polysaccharide-based	126:145	arg1	hydrogel					147:154	Polysaccharide-based hydrogel	126:154	Polysaccharide-based hydrogel	126:154	Polysaccharide-based hydrogel adsorbents become popular because of their high adsorption capacity and fast adsorption rate, but their low removal rate and poor pH resistance have always been fatal shortcomings.					
37105247	2	27	theme	organic	436:442	arg1	pollutants					444:453	organic pollutants	436:453	organic pollutants (i.e., dye)	436:465	Herein, a feasible strategy was proposed to strengthen the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure.					
37105247	0	28	theme	rigid-in-flexible	41:57	arg1	structure					67:75	a rigid-in-flexible network structure	39:75	a rigid-in-flexible network structure	39:75	Chitosan-based composite hydrogel with a rigid-in-flexible network structure for pH-universal ultra-efficient removal of dye.					
37105247	1	29	theme	fatal	317:321	arg1	shortcomings					323:334	fatal shortcomings	317:334	fatal shortcomings	317:334	Polysaccharide-based hydrogel adsorbents become popular because of their high adsorption capacity and fast adsorption rate, but their low removal rate and poor pH resistance have always been fatal shortcomings.					
37105247	2	30	theme	hydrogel	575:582	arg1	network					584:590	chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network	513:590	chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure	513:639	Herein, a feasible strategy was proposed to strengthen the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure.					
37105247	2	31	theme	adsorbent	416:424	arg1	hydrogel					407:414	hydrogel adsorbent	407:424	hydrogel adsorbent	407:424	Herein, a feasible strategy was proposed to strengthen the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure.					
37105247	3	32	theme	commercial	1047:1056	arg1	carbons					1068:1074	commercial activated carbons	1047:1074	commercial activated carbons	1047:1074	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	33	theme	REC	677:679	arg1	dosage					667:672	less dosage	662:672	less dosage of REC (1.2 wt%)	662:689	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	34	theme	tap	805:807	arg1	water					809:813	tap water	805:813	tap water	805:813	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	4	35	theme	broad	1129:1133	arg1	2-11					1145:1148	2-11	1145:1148	2-11	1145:1148	The adsorbent keeps high adsorption efficiency in a broad pH range (2-11), and can be reused for >4 times.					
37105247	4	35	theme	broad	1129:1133	arg1	range					1138:1142	a broad pH range	1127:1142	a broad pH range (2-11)	1127:1149	The adsorbent keeps high adsorption efficiency in a broad pH range (2-11), and can be reused for >4 times.					
37105247	2	36	theme	network	623:629	arg1	structure					631:639	a rectorite-in-polymer network structure	600:639	a rectorite-in-polymer network structure	600:639	Herein, a feasible strategy was proposed to strengthen the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure.					
37105247	0	37	with	hydrogel	25:32	arg1	structure					67:75	a rigid-in-flexible network structure	39:75	a rigid-in-flexible network structure	39:75	Chitosan-based composite hydrogel with a rigid-in-flexible network structure for pH-universal ultra-efficient removal of dye.					
37105247	0	38	theme	network	59:65	arg1	structure					67:75	a rigid-in-flexible network structure	39:75	a rigid-in-flexible network structure	39:75	Chitosan-based composite hydrogel with a rigid-in-flexible network structure for pH-universal ultra-efficient removal of dye.					
37105247	2	39	theme	rectorite-in-polymer	602:621	arg1	structure					631:639	a rectorite-in-polymer network structure	600:639	a rectorite-in-polymer network structure	600:639	Herein, a feasible strategy was proposed to strengthen the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure.					
37105247	4	40	theme	>4	1174:1175	arg1	times					1177:1181	>4 times	1174:1181	>4 times	1174:1181	The adsorbent keeps high adsorption efficiency in a broad pH range (2-11), and can be reused for >4 times.					
37105247	3	41	theme	15.8 wt	937:943	arg1	REC					946:948	15.8 wt% REC	937:948	15.8 wt% REC	937:948	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	41	theme	15.8 wt	937:943	arg1	REC					932:934	REC	932:934	REC (15.8 wt% REC)	932:949	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	42	theme	Yellow	851:856	arg1	1083.39-1303.49 mg/g					871:890	1083.39-1303.49 mg/g	871:890	1083.39-1303.49 mg/g	871:890	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	42	theme	Yellow	851:856	arg1	water					864:868	Yellow River water	851:868	Yellow River water (1083.39-1303.49 mg/g)	851:891	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	43	theme	deionized	788:796	arg1	water					798:802	deionized water	788:802	deionized water	788:802	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	44	theme	dosage	667:672	arg1	introduction					646:657	The introduction	642:657	The introduction of less dosage of REC (1.2 wt%) into the hydrogel	642:707	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	45	theme	%	944:944	arg1	REC					946:948	15.8 wt% REC	937:948	15.8 wt% REC	937:948	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	45	theme	%	944:944	arg1	REC					932:934	REC	932:934	REC (15.8 wt% REC)	932:949	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	2	46	theme	feasible	347:354	arg1	strategy					356:363	a feasible strategy	345:363	a feasible strategy	345:363	Herein, a feasible strategy was proposed to strengthen the ability of hydrogel adsorbent to remove organic pollutants (i.e., dye) by incorporating natural rectorite (REC) into chitosan-g-poly (2-acrylamido-2-methyl-propane-sulfonic-acid) hydrogel network to form a rectorite-in-polymer network structure.					
37105247	3	47	theme	River	858:862	arg1	1083.39-1303.49 mg/g					871:890	1083.39-1303.49 mg/g	871:890	1083.39-1303.49 mg/g	871:890	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	3	47	theme	River	858:862	arg1	water					864:868	Yellow River water	851:868	Yellow River water (1083.39-1303.49 mg/g)	851:891	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	4	48	theme	pH	1135:1136	arg1	2-11					1145:1148	2-11	1145:1148	2-11	1145:1148	The adsorbent keeps high adsorption efficiency in a broad pH range (2-11), and can be reused for >4 times.					
37105247	4	48	theme	pH	1135:1136	arg1	range					1138:1142	a broad pH range	1127:1142	a broad pH range (2-11)	1127:1149	The adsorbent keeps high adsorption efficiency in a broad pH range (2-11), and can be reused for >4 times.					
37105247	4	49	from	efficiency	1113:1122	arg1	2-11					1145:1148	2-11	1145:1148	2-11	1145:1148	The adsorbent keeps high adsorption efficiency in a broad pH range (2-11), and can be reused for >4 times.					
37105247	4	49	from	efficiency	1113:1122	arg1	range					1138:1142	a broad pH range	1127:1142	a broad pH range (2-11)	1127:1149	The adsorbent keeps high adsorption efficiency in a broad pH range (2-11), and can be reused for >4 times.					
37105247	4	50	theme	adsorption	1102:1111	arg1	efficiency					1113:1122	high adsorption efficiency	1097:1122	high adsorption efficiency in a broad pH range (2-11)	1097:1149	The adsorbent keeps high adsorption efficiency in a broad pH range (2-11), and can be reused for >4 times.					
37105247	3	51	theme	activated	1058:1066	arg1	carbons					1068:1074	commercial activated carbons	1047:1074	commercial activated carbons	1047:1074	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	1	52	theme	low	260:262	arg1	rate					272:275	their low removal rate	254:275	their low removal rate	254:275	Polysaccharide-based hydrogel adsorbents become popular because of their high adsorption capacity and fast adsorption rate, but their low removal rate and poor pH resistance have always been fatal shortcomings.					
37105247	0	53	theme	pH-universal	81:92	arg1	removal					110:116	pH-universal ultra-efficient removal	81:116	pH-universal ultra-efficient removal of dye	81:123	Chitosan-based composite hydrogel with a rigid-in-flexible network structure for pH-universal ultra-efficient removal of dye.					
37105247	4	54	theme	high	1097:1100	arg1	efficiency					1113:1122	high adsorption efficiency	1097:1122	high adsorption efficiency in a broad pH range (2-11)	1097:1149	The adsorbent keeps high adsorption efficiency in a broad pH range (2-11), and can be reused for >4 times.					
37105247	3	55	dep	rate	980:983	arg1	%					991:991	99.6 %	986:991	99.6 % for MB in real waters	986:1013	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
37105247	1	56	theme	removal	264:270	arg1	rate					272:275	their low removal rate	254:275	their low removal rate	254:275	Polysaccharide-based hydrogel adsorbents become popular because of their high adsorption capacity and fast adsorption rate, but their low removal rate and poor pH resistance have always been fatal shortcomings.					
37105247	3	57	theme	less	662:665	arg1	dosage					667:672	less dosage	662:672	less dosage of REC (1.2 wt%)	662:689	The introduction of less dosage of REC (1.2 wt%) into the hydrogel facilitates to improve its adsorption capacities toward methylene blue (MB) in deionized water, tap water, seawater, Yangtze River water, and Yellow River water (1083.39-1303.49 mg/g); while incorporating higher content of REC (15.8 wt% REC) helps to improve the removal rate (99.6 % for MB in real waters), which are greatly superior to commercial activated carbons.					
36933133	6	0	theme	composite	1114:1122	arg1	structure					1124:1132	CTSN composite structure	1109:1132	CTSN composite structure	1109:1132	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	11	1	theme	effective	1828:1836	arg1	light					1846:1850	effective visible light	1828:1850	effective visible light	1828:1850	This study provides a simple route towards the designing of rapid, effective visible light oriented photocatalyts for the existing environmental issues.					
36933133	6	2	theme	fluffy	949:954	arg1	sheets					956:961	fine fluffy sheets	944:961	fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure	944:1132	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	6	3	dep	structure	968:976	arg1	500 nm					986:991	500 nm	986:991	500 nm	986:991	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	7	4	theme	bandgap	1139:1145	arg1	2.94					1233:1236	2.94	1233:1236	2.94	1233:1236	The bandgap energies for g-C3N4, CTSN/g-C3N4, and Pt@ CTSN/g-C3N4 photocatalysts were found to be 2.94, 2.73, and 2.72 eV, respectively.					
36933133	7	4	theme	bandgap	1139:1145	arg1	energies					1147:1154	The bandgap energies	1135:1154	The bandgap energies for g-C3N4, CTSN/g-C3N4, and Pt@ CTSN/g-C3N4 photocatalysts	1135:1214	The bandgap energies for g-C3N4, CTSN/g-C3N4, and Pt@ CTSN/g-C3N4 photocatalysts were found to be 2.94, 2.73, and 2.72 eV, respectively.					
36933133	4	5	from	involvement	705:715	arg1	nitride					767:773	graphitic carbon nitride	750:773	graphitic carbon nitride	750:773	XRD results confirmed the involvement of α-polymorphic form of CTSN in graphitic carbon nitride.					
36933133	9	6	theme	Pt	1430:1431	arg1	efficacious					1483:1493	efficacious	1483:1493	efficacious	1483:1493	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	9	6	theme	Pt	1430:1431	arg1	photocatalyst					1453:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst	1410:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst	1410:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	6	7	from	dispersion	1064:1073	arg1	structure					1124:1132	CTSN composite structure	1109:1132	CTSN composite structure	1109:1132	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	6	7	from	dispersion	1064:1073	arg1	g-C3N4					1098:1103	g-C3N4	1098:1103	g-C3N4	1098:1103	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	0	8	theme	Rapid	0:4	arg1	elimination					6:16	Rapid elimination	0:16	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt	0:79	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt nanoparticles dispersed chitosan/g-C3N4 ternary visible light photocatalyst.					
36933133	9	9	theme	CTSN/g-C3N4	1433:1443	arg1	efficacious					1483:1493	efficacious	1483:1493	efficacious	1483:1493	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	9	9	theme	CTSN/g-C3N4	1433:1443	arg1	photocatalyst					1453:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst	1410:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst	1410:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	6	10	theme	synthesized	915:925	arg1	g-C3N4					927:932	the synthesized g-C3N4	911:932	the synthesized g-C3N4	911:932	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	9	11	from	elimination	1503:1513	arg1	MB					1562:1563	MB	1562:1563	MB	1562:1563	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	9	11	from	elimination	1503:1513	arg1	%					1570:1570	95.2%	1566:1570	95.2%	1566:1570	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	9	11	from	elimination	1503:1513	arg1	25 min					1551:1556	25 min	1551:1556	25 min	1551:1556	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	2	12	theme	visible	353:359	arg1	light					361:365	well-organized visible light	338:365	well-organized visible light	338:365	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	1	13	theme	modification	225:236	arg1	selection					179:187	Appropriate material selection	158:187	Appropriate material selection	158:187	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	1	13	theme	modification	225:236	arg1	understanding					200:212	proper understanding	193:212	proper understanding of bandgap modification	193:236	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	1	13	theme	modification	225:236	arg1	factors					246:252	key factors	242:252	key factors for the development of efficient photocatalysts	242:300	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	0	14	theme	light	137:141	arg1	photocatalyst					143:155	dispersed chitosan/g-C3N4 ternary visible light photocatalyst	95:155	dispersed chitosan/g-C3N4 ternary visible light photocatalyst	95:155	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt nanoparticles dispersed chitosan/g-C3N4 ternary visible light photocatalyst.					
36933133	1	15	theme	key	242:244	arg1	selection					179:187	Appropriate material selection	158:187	Appropriate material selection	158:187	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	1	15	theme	key	242:244	arg1	understanding					200:212	proper understanding	193:212	proper understanding of bandgap modification	193:236	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	1	15	theme	key	242:244	arg1	factors					246:252	key factors	242:252	key factors for the development of efficient photocatalysts	242:300	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	4	16	theme	XRD	679:681	arg1	results					683:689	XRD results	679:689	XRD results	679:689	XRD results confirmed the involvement of α-polymorphic form of CTSN in graphitic carbon nitride.					
36933133	3	17	theme	FTIR	599:602	arg1	spectroscopy					604:615	FTIR spectroscopy	599:615	FTIR spectroscopy	599:615	Modern techniques like XRD, XPS, TEM, FESEM, UV-Vis, and FTIR spectroscopy were exploited for characterization of synthesized materials.					
36933133	8	18	theme	created	1308:1314	arg1	structure					1316:1324	each created structure	1303:1324	each created structure	1303:1324	The photodegradation skills of each created structure have been examined on antibiotic gemifloxacin mesylate and methylene blue (MB) dye.					
36933133	1	19	theme	Appropriate	158:168	arg1	selection					179:187	Appropriate material selection	158:187	Appropriate material selection	158:187	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	1	19	theme	Appropriate	158:168	arg1	understanding					200:212	proper understanding	193:212	proper understanding of bandgap modification	193:236	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	1	19	theme	Appropriate	158:168	arg1	factors					246:252	key factors	242:252	key factors for the development of efficient photocatalysts	242:300	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	4	20	theme	carbon	760:765	arg1	nitride					767:773	graphitic carbon nitride	750:773	graphitic carbon nitride	750:773	XRD results confirmed the involvement of α-polymorphic form of CTSN in graphitic carbon nitride.					
36933133	4	21	theme	α-polymorphic	720:732	arg1	form					734:737	α-polymorphic form	720:737	α-polymorphic form of CTSN	720:745	XRD results confirmed the involvement of α-polymorphic form of CTSN in graphitic carbon nitride.					
36933133	0	22	theme	blue	68:71	arg1	methylene					58:66	methylene blue	58:71	methylene blue	58:71	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt nanoparticles dispersed chitosan/g-C3N4 ternary visible light photocatalyst.					
36933133	9	23	theme	gemifloxacin	1518:1529	arg1	mesylate					1531:1538	gemifloxacin mesylate	1518:1538	gemifloxacin mesylate (93.3%)	1518:1546	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	9	23	theme	gemifloxacin	1518:1529	arg1	%					1545:1545	93.3%	1541:1545	93.3%	1541:1545	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	2	24	dep	efficient	327:335	arg1	oriented					367:374	oriented	367:374	oriented	367:374	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	1	25	theme	proper	193:198	arg1	selection					179:187	Appropriate material selection	158:187	Appropriate material selection	158:187	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	1	25	theme	proper	193:198	arg1	understanding					200:212	proper understanding	193:212	proper understanding of bandgap modification	193:236	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	1	25	theme	proper	193:198	arg1	factors					246:252	key factors	242:252	key factors for the development of efficient photocatalysts	242:300	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	6	26	theme	dense	1022:1026	arg1	framework					1036:1044	a dense layered framework	1020:1044	a dense layered framework of CTSN	1020:1052	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	8	27	theme	antibiotic	1348:1357	arg1	mesylate					1372:1379	antibiotic gemifloxacin mesylate	1348:1379	antibiotic gemifloxacin mesylate	1348:1379	The photodegradation skills of each created structure have been examined on antibiotic gemifloxacin mesylate and methylene blue (MB) dye.					
36933133	0	28	theme	dispersed	95:103	arg1	photocatalyst					143:155	dispersed chitosan/g-C3N4 ternary visible light photocatalyst	95:155	dispersed chitosan/g-C3N4 ternary visible light photocatalyst	95:155	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt nanoparticles dispersed chitosan/g-C3N4 ternary visible light photocatalyst.					
36933133	2	29	theme	chitosan	447:454	arg1	CTSN					457:460	CTSN	457:460	CTSN	457:460	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	2	29	theme	chitosan	447:454	arg1	network					436:442	polymeric network	426:442	polymeric network of chitosan (CTSN)	426:461	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	2	29	theme	chitosan	447:454	arg1	nanoparticles					481:493	platinum (Pt) nanoparticles	467:493	platinum (Pt) nanoparticles utilizing a straightforward chemical approach	467:539	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	0	30	theme	ternary	121:127	arg1	photocatalyst					143:155	dispersed chitosan/g-C3N4 ternary visible light photocatalyst	95:155	dispersed chitosan/g-C3N4 ternary visible light photocatalyst	95:155	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt nanoparticles dispersed chitosan/g-C3N4 ternary visible light photocatalyst.					
36933133	0	31	theme	mesylate	45:52	arg1	elimination					6:16	Rapid elimination	0:16	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt	0:79	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt nanoparticles dispersed chitosan/g-C3N4 ternary visible light photocatalyst.					
36933133	6	32	theme	CTSN	1049:1052	arg1	framework					1036:1044	a dense layered framework	1020:1044	a dense layered framework of CTSN	1020:1052	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	7	33	theme	CTSN/g-C3N4	1189:1199	arg1	photocatalysts					1201:1214	Pt@ CTSN/g-C3N4 photocatalysts	1185:1214	Pt@ CTSN/g-C3N4 photocatalysts	1185:1214	The bandgap energies for g-C3N4, CTSN/g-C3N4, and Pt@ CTSN/g-C3N4 photocatalysts were found to be 2.94, 2.73, and 2.72 eV, respectively.					
36933133	6	34	theme	nanoparticles	1081:1093	arg1	dispersion					1064:1073	good dispersion	1059:1073	good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure	1059:1132	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	10	35	theme	photocatalytic	1641:1654	arg1	framework					1656:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework	1609:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework	1609:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework exhibited ⁓ 2.20 times more effective than bare g-C3N4 for the destruction of antibiotic drug.					
36933133	7	36	theme	Pt	1185:1186	arg1	photocatalysts					1201:1214	Pt@ CTSN/g-C3N4 photocatalysts	1185:1214	Pt@ CTSN/g-C3N4 photocatalysts	1185:1214	The bandgap energies for g-C3N4, CTSN/g-C3N4, and Pt@ CTSN/g-C3N4 photocatalysts were found to be 2.94, 2.73, and 2.72 eV, respectively.					
36933133	2	37	theme	straightforward	507:521	arg1	approach					532:539	a straightforward chemical approach	505:539	a straightforward chemical approach	505:539	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	11	38	theme	existing	1883:1890	arg1	issues					1906:1911	the existing environmental issues	1879:1911	the existing environmental issues	1879:1911	This study provides a simple route towards the designing of rapid, effective visible light oriented photocatalyts for the existing environmental issues.					
36933133	10	39	theme	CTSN/g-C3N4	1621:1631	arg1	framework					1656:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework	1609:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework	1609:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework exhibited ⁓ 2.20 times more effective than bare g-C3N4 for the destruction of antibiotic drug.					
36933133	0	40	theme	gemifloxacin	32:43	arg1	mesylate					45:52	antibiotic gemifloxacin mesylate	21:52	antibiotic gemifloxacin mesylate	21:52	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt nanoparticles dispersed chitosan/g-C3N4 ternary visible light photocatalyst.					
36933133	5	41	theme	photocatalytic	830:843	arg1	structure					845:853	trio photocatalytic structure	825:853	trio photocatalytic structure	825:853	XPS investigation confirmed the establishment of trio photocatalytic structure among Pt, CTSN, and g-C3N4.					
36933133	6	42	theme	good	1059:1062	arg1	dispersion					1064:1073	good dispersion	1059:1073	good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure	1059:1132	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	3	43	theme	materials	668:676	arg1	characterization					636:651	characterization	636:651	characterization of synthesized materials	636:676	Modern techniques like XRD, XPS, TEM, FESEM, UV-Vis, and FTIR spectroscopy were exploited for characterization of synthesized materials.					
36933133	6	44	theme	TEM	883:885	arg1	examination					887:897	TEM examination	883:897	TEM examination	883:897	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	6	45	from	500 nm	986:991	arg1	size					996:999	size	996:999	size	996:999	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	10	46	theme	bare	1709:1712	arg1	g-C3N4					1714:1719	bare g-C3N4	1709:1719	bare g-C3N4 for the destruction of antibiotic drug	1709:1758	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework exhibited ⁓ 2.20 times more effective than bare g-C3N4 for the destruction of antibiotic drug.					
36933133	11	47	theme	environmental	1892:1904	arg1	issues					1906:1911	the existing environmental issues	1879:1911	the existing environmental issues	1879:1911	This study provides a simple route towards the designing of rapid, effective visible light oriented photocatalyts for the existing environmental issues.					
36933133	11	48	theme	visible	1838:1844	arg1	light					1846:1850	effective visible light	1828:1850	effective visible light	1828:1850	This study provides a simple route towards the designing of rapid, effective visible light oriented photocatalyts for the existing environmental issues.					
36933133	8	49	dep	blue	1395:1398	arg1	MB					1401:1402	MB	1401:1402	MB	1401:1402	The photodegradation skills of each created structure have been examined on antibiotic gemifloxacin mesylate and methylene blue (MB) dye.					
36933133	5	50	theme	structure	845:853	arg1	establishment					808:820	the establishment	804:820	the establishment of trio photocatalytic structure among Pt, CTSN, and g-C3N4	804:880	XPS investigation confirmed the establishment of trio photocatalytic structure among Pt, CTSN, and g-C3N4.					
36933133	9	51	theme	visible	1594:1600	arg1	light					1602:1606	visible light	1594:1606	visible light	1594:1606	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	1	52	theme	photocatalysts	287:300	arg1	development					262:272	the development	258:272	the development of efficient photocatalysts	258:300	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	6	53	theme	CTSN	1109:1112	arg1	structure					1124:1132	CTSN composite structure	1109:1132	CTSN composite structure	1109:1132	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	2	54	from	g-C3N4	399:404	arg1	association					409:419	association	409:419	association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach	409:539	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	2	55	theme	efficient	327:335	arg1	photocatalyst					376:388	an efficient, well-organized visible light oriented photocatalyst	324:388	an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach	324:539	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	9	56	theme	developed	1420:1428	arg1	efficacious					1483:1493	efficacious	1483:1493	efficacious	1483:1493	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	9	56	theme	developed	1420:1428	arg1	photocatalyst					1453:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst	1410:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst	1410:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	6	57	theme	fine	944:947	arg1	sheets					956:961	fine fluffy sheets	944:961	fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure	944:1132	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	1	58	theme	bandgap	217:223	arg1	modification					225:236	bandgap modification	217:236	bandgap modification	217:236	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	9	59	theme	@	1432:1432	arg1	efficacious					1483:1493	efficacious	1483:1493	efficacious	1483:1493	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	9	59	theme	@	1432:1432	arg1	photocatalyst					1453:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst	1410:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst	1410:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	10	60	theme	drug	1755:1758	arg1	destruction					1729:1739	the destruction	1725:1739	the destruction of antibiotic drug	1725:1758	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework exhibited ⁓ 2.20 times more effective than bare g-C3N4 for the destruction of antibiotic drug.					
36933133	9	61	theme	ternary	1445:1451	arg1	efficacious					1483:1493	efficacious	1483:1493	efficacious	1483:1493	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	9	61	theme	ternary	1445:1451	arg1	photocatalyst					1453:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst	1410:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst	1410:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	9	62	from	efficacious	1483:1493	arg1	18 min					1581:1586	18 min	1581:1586	18 min	1581:1586	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	2	63	theme	well-organized	338:351	arg1	light					361:365	well-organized visible light	338:365	well-organized visible light	338:365	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	10	64	theme	antibiotic	1744:1753	arg1	drug					1755:1758	antibiotic drug	1744:1758	antibiotic drug	1744:1758	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework exhibited ⁓ 2.20 times more effective than bare g-C3N4 for the destruction of antibiotic drug.					
36933133	11	65	theme	rapid	1821:1825	arg1	photocatalyts					1861:1873	rapid, effective visible light oriented photocatalyts	1821:1873	rapid, effective visible light oriented photocatalyts	1821:1873	This study provides a simple route towards the designing of rapid, effective visible light oriented photocatalyts for the existing environmental issues.					
36933133	8	66	theme	photodegradation	1276:1291	arg1	skills					1293:1298	The photodegradation skills	1272:1298	The photodegradation skills of each created structure	1272:1324	The photodegradation skills of each created structure have been examined on antibiotic gemifloxacin mesylate and methylene blue (MB) dye.					
36933133	4	67	theme	CTSN	742:745	arg1	form					734:737	α-polymorphic form	720:737	α-polymorphic form of CTSN	720:745	XRD results confirmed the involvement of α-polymorphic form of CTSN in graphitic carbon nitride.					
36933133	11	68	theme	simple	1783:1788	arg1	route					1790:1794	a simple route	1781:1794	a simple route towards the designing of rapid, effective visible light oriented photocatalyts for the existing environmental issues	1781:1911	This study provides a simple route towards the designing of rapid, effective visible light oriented photocatalyts for the existing environmental issues.					
36933133	4	69	theme	graphitic	750:758	arg1	nitride					767:773	graphitic carbon nitride	750:773	graphitic carbon nitride	750:773	XRD results confirmed the involvement of α-polymorphic form of CTSN in graphitic carbon nitride.					
36933133	6	70	dep	500 nm	986:991	arg1	to					983:984	to	983:984	to	983:984	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	1	71	theme	material	170:177	arg1	selection					179:187	Appropriate material selection	158:187	Appropriate material selection	158:187	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	1	71	theme	material	170:177	arg1	understanding					200:212	proper understanding	193:212	proper understanding of bandgap modification	193:236	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	1	71	theme	material	170:177	arg1	factors					246:252	key factors	242:252	key factors for the development of efficient photocatalysts	242:300	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
36933133	8	72	theme	structure	1316:1324	arg1	skills					1293:1298	The photodegradation skills	1272:1298	The photodegradation skills of each created structure	1272:1324	The photodegradation skills of each created structure have been examined on antibiotic gemifloxacin mesylate and methylene blue (MB) dye.					
36933133	9	73	theme	mesylate	1531:1538	arg1	elimination					1503:1513	the elimination	1499:1513	the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%)	1499:1571	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	6	74	theme	layered	1028:1034	arg1	framework					1036:1044	a dense layered framework	1020:1044	a dense layered framework of CTSN	1020:1052	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	2	75	theme	chemical	523:530	arg1	approach					532:539	a straightforward chemical approach	505:539	a straightforward chemical approach	505:539	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	4	76	theme	form	734:737	arg1	involvement					705:715	the involvement	701:715	the involvement of α-polymorphic form of CTSN in graphitic carbon nitride	701:773	XRD results confirmed the involvement of α-polymorphic form of CTSN in graphitic carbon nitride.					
36933133	0	77	theme	chitosan/g-C3N4	105:119	arg1	photocatalyst					143:155	dispersed chitosan/g-C3N4 ternary visible light photocatalyst	95:155	dispersed chitosan/g-C3N4 ternary visible light photocatalyst	95:155	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt nanoparticles dispersed chitosan/g-C3N4 ternary visible light photocatalyst.					
36933133	0	78	theme	visible	129:135	arg1	photocatalyst					143:155	dispersed chitosan/g-C3N4 ternary visible light photocatalyst	95:155	dispersed chitosan/g-C3N4 ternary visible light photocatalyst	95:155	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt nanoparticles dispersed chitosan/g-C3N4 ternary visible light photocatalyst.					
36933133	8	79	theme	gemifloxacin	1359:1370	arg1	mesylate					1372:1379	antibiotic gemifloxacin mesylate	1348:1379	antibiotic gemifloxacin mesylate	1348:1379	The photodegradation skills of each created structure have been examined on antibiotic gemifloxacin mesylate and methylene blue (MB) dye.					
36933133	5	80	theme	XPS	776:778	arg1	investigation					780:792	XPS investigation	776:792	XPS investigation	776:792	XPS investigation confirmed the establishment of trio photocatalytic structure among Pt, CTSN, and g-C3N4.					
36933133	7	81	theme	@	1187:1187	arg1	photocatalysts					1201:1214	Pt@ CTSN/g-C3N4 photocatalysts	1185:1214	Pt@ CTSN/g-C3N4 photocatalysts	1185:1214	The bandgap energies for g-C3N4, CTSN/g-C3N4, and Pt@ CTSN/g-C3N4 photocatalysts were found to be 2.94, 2.73, and 2.72 eV, respectively.					
36933133	9	82	from	18 min	1581:1586	arg1	efficacious					1483:1493	efficacious	1483:1493	efficacious	1483:1493	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	9	82	from	18 min	1581:1586	arg1	photocatalyst					1453:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst	1410:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst	1410:1465	The newly developed Pt@CTSN/g-C3N4 ternary photocatalyst was found to be efficacious for the elimination of gemifloxacin mesylate (93.3%) in 25 min and MB (95.2%) just in 18 min under visible light.					
36933133	2	83	dep	oriented	367:374	arg1	light					361:365	well-organized visible light	338:365	well-organized visible light	338:365	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	2	84	theme	polymeric	426:434	arg1	CTSN					457:460	CTSN	457:460	CTSN	457:460	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	2	84	theme	polymeric	426:434	arg1	network					436:442	polymeric network	426:442	polymeric network of chitosan (CTSN)	426:461	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	0	85	theme	antibiotic	21:30	arg1	mesylate					45:52	antibiotic gemifloxacin mesylate	21:52	antibiotic gemifloxacin mesylate	21:52	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt nanoparticles dispersed chitosan/g-C3N4 ternary visible light photocatalyst.					
36933133	6	86	theme	Pt	1078:1079	arg1	nanoparticles					1081:1093	Pt nanoparticles	1078:1093	Pt nanoparticles	1078:1093	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	2	87	theme	platinum	467:474	arg1	nanoparticles					481:493	platinum (Pt) nanoparticles	467:493	platinum (Pt) nanoparticles utilizing a straightforward chemical approach	467:539	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	8	88	theme	blue	1395:1398	arg1	dye					1405:1407	methylene blue (MB) dye	1385:1407	methylene blue (MB) dye	1385:1407	The photodegradation skills of each created structure have been examined on antibiotic gemifloxacin mesylate and methylene blue (MB) dye.					
36933133	10	89	theme	ternary	1633:1639	arg1	framework					1656:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework	1609:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework	1609:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework exhibited ⁓ 2.20 times more effective than bare g-C3N4 for the destruction of antibiotic drug.					
36933133	5	90	theme	trio	825:828	arg1	structure					845:853	trio photocatalytic structure	825:853	trio photocatalytic structure	825:853	XPS investigation confirmed the establishment of trio photocatalytic structure among Pt, CTSN, and g-C3N4.					
36933133	6	91	contain	possesses	934:942	arg2	sheets					956:961	fine fluffy sheets	944:961	fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure	944:1132	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	6	91	contain	possesses	934:942	arg1	g-C3N4					927:932	the synthesized g-C3N4	911:932	the synthesized g-C3N4	911:932	TEM examination showed that the synthesized g-C3N4 possesses fine fluffy sheets like structure (100 to 500 nm in size) intermingled with a dense layered framework of CTSN with good dispersion of Pt nanoparticles on g-C3N4 and CTSN composite structure.					
36933133	11	92	dep	oriented	1852:1859	arg1	light					1846:1850	effective visible light	1828:1850	effective visible light	1828:1850	This study provides a simple route towards the designing of rapid, effective visible light oriented photocatalyts for the existing environmental issues.					
36933133	10	93	theme	Pt	1618:1619	arg1	framework					1656:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework	1609:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework	1609:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework exhibited ⁓ 2.20 times more effective than bare g-C3N4 for the destruction of antibiotic drug.					
36933133	3	94	theme	synthesized	656:666	arg1	materials					668:676	synthesized materials	656:676	synthesized materials	656:676	Modern techniques like XRD, XPS, TEM, FESEM, UV-Vis, and FTIR spectroscopy were exploited for characterization of synthesized materials.					
36933133	10	95	theme	@	1620:1620	arg1	framework					1656:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework	1609:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework	1609:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework exhibited ⁓ 2.20 times more effective than bare g-C3N4 for the destruction of antibiotic drug.					
36933133	0	96	theme	methylene	58:66	arg1	elimination					6:16	Rapid elimination	0:16	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt	0:79	Rapid elimination of antibiotic gemifloxacin mesylate and methylene blue over Pt nanoparticles dispersed chitosan/g-C3N4 ternary visible light photocatalyst.					
36933133	3	97	theme	Modern	542:547	arg1	techniques					549:558	Modern techniques	542:558	Modern techniques like XRD, XPS, TEM, FESEM, UV-Vis, and FTIR spectroscopy	542:615	Modern techniques like XRD, XPS, TEM, FESEM, UV-Vis, and FTIR spectroscopy were exploited for characterization of synthesized materials.					
36933133	11	98	dep	rapid	1821:1825	arg1	oriented					1852:1859	oriented	1852:1859	oriented	1852:1859	This study provides a simple route towards the designing of rapid, effective visible light oriented photocatalyts for the existing environmental issues.					
36933133	2	99	with	association	409:419	arg1	CTSN					457:460	CTSN	457:460	CTSN	457:460	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	2	99	with	association	409:419	arg1	network					436:442	polymeric network	426:442	polymeric network of chitosan (CTSN)	426:461	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	2	99	with	association	409:419	arg1	nanoparticles					481:493	platinum (Pt) nanoparticles	467:493	platinum (Pt) nanoparticles utilizing a straightforward chemical approach	467:539	Herein, we developed an efficient, well-organized visible light oriented photocatalyst based on g-C3N4 in association with polymeric network of chitosan (CTSN) and platinum (Pt) nanoparticles utilizing a straightforward chemical approach.					
36933133	10	100	theme	Designed	1609:1616	arg1	framework					1656:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework	1609:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework	1609:1664	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework exhibited ⁓ 2.20 times more effective than bare g-C3N4 for the destruction of antibiotic drug.					
36933133	10	101	theme	effective	1694:1702	arg1	times					1683:1687	⁓ 2.20 times	1676:1687	⁓ 2.20 times more effective	1676:1702	Designed Pt@CTSN/g-C3N4 ternary photocatalytic framework exhibited ⁓ 2.20 times more effective than bare g-C3N4 for the destruction of antibiotic drug.					
36933133	1	102	theme	efficient	277:285	arg1	photocatalysts					287:300	efficient photocatalysts	277:300	efficient photocatalysts	277:300	Appropriate material selection and proper understanding of bandgap modification are key factors for the development of efficient photocatalysts.					
35731847	6	0	theme	Further	930:936	arg1	analysis					938:945	Further analysis	930:945	Further analysis	930:945	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	7	1	theme	peptide-based	1318:1330	arg1	hydrogel					1332:1339	This peptide-based hydrogel	1313:1339	This peptide-based hydrogel	1313:1339	This peptide-based hydrogel provides a novel intervention for the treatment of chronic skin wounds and shows promise as a wound dressing in the field of tissue regeneration.					
35731847	6	2	theme	skin	1212:1215	arg1	wounds					1217:1222	full-thickness skin wounds	1197:1222	full-thickness skin wounds	1197:1222	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	2	3	theme	considerable	369:380	arg1	attention					382:390	considerable attention	369:390	considerable attention	369:390	Many studies have explored novel interventions against skin wounds, with bioactive peptides, nanoparticles, and hydrogels arousing considerable attention regarding their therapeutic potential.					
35731847	6	4	from	wounds	1297:1302	arg1	mice					1307:1310	mice	1307:1310	mice	1307:1310	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	6	5	theme	scratch	1025:1031	arg1	repair					1033:1038	keratinocyte scratch repair	1012:1038	keratinocyte scratch repair	1012:1038	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	6	6	theme	chronic	1289:1295	arg1	wounds					1297:1302	biofilm-infected chronic wounds	1272:1302	methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice	1228:1310	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	6	7	theme	wounds	1217:1222	arg1	healing					1181:1187	the rapid healing	1171:1187	the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice	1171:1310	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	7	8	theme	regeneration	1473:1484	arg1	field					1457:1461	the field	1453:1461	the field of tissue regeneration	1453:1484	This peptide-based hydrogel provides a novel intervention for the treatment of chronic skin wounds and shows promise as a wound dressing in the field of tissue regeneration.					
35731847	6	9	theme	keratinocyte	1012:1023	arg1	repair					1033:1038	keratinocyte scratch repair	1012:1038	keratinocyte scratch repair	1012:1038	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	0	10	theme	Promising	102:110	arg1	Treatment					112:120	Promising Treatment	102:120	Promising Treatment for Chronic Skin Wounds	102:144	Zn2+ Cross-Linked Alginate Carrying Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides Provides Promising Treatment for Chronic Skin Wounds.					
35731847	4	11	theme	hydrogel	716:723	arg1	composites					725:734	the hydrogel composites	712:734	the hydrogel composites	712:734	The characteristics, biological properties, and safety profiles of the hydrogel composites were then evaluated.					
35731847	0	12	theme	Chronic	126:132	arg1	Wounds					139:144	Chronic Skin Wounds	126:144	Chronic Skin Wounds	126:144	Zn2+ Cross-Linked Alginate Carrying Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides Provides Promising Treatment for Chronic Skin Wounds.					
35731847	7	13	theme	skin	1400:1403	arg1	wounds					1405:1410	chronic skin wounds	1392:1410	chronic skin wounds	1392:1410	This peptide-based hydrogel provides a novel intervention for the treatment of chronic skin wounds and shows promise as a wound dressing in the field of tissue regeneration.					
35731847	3	14	theme	@	543:543	arg1	nanocomposites					552:565	these HSN@RL-QN15 nanocomposites	534:565	these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel	534:642	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	6	15	theme	re-epithelialization	1103:1122	arg1	formation					1147:1155	accelerated re-epithelialization and granulation tissue formation	1091:1155	accelerated re-epithelialization and granulation tissue formation	1091:1155	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	6	16	theme	granulation	1128:1138	arg1	tissue					1140:1145	granulation tissue	1128:1145	granulation tissue	1128:1145	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	5	17	theme	loaded	914:919	arg1	RL-QN15					921:927	loaded RL-QN15	914:927	loaded RL-QN15	914:927	Results showed that the hydrogel had good porosity, hemocompatibility, biocompatibility, and broad-spectrum antimicrobial activity, with the slow release of loaded RL-QN15.					
35731847	2	18	theme	skin	293:296	arg1	wounds					298:303	skin wounds	293:303	skin wounds	293:303	Many studies have explored novel interventions against skin wounds, with bioactive peptides, nanoparticles, and hydrogels arousing considerable attention regarding their therapeutic potential.					
35731847	6	19	theme	tissue	1140:1145	arg1	formation					1147:1155	accelerated re-epithelialization and granulation tissue formation	1091:1155	accelerated re-epithelialization and granulation tissue formation	1091:1155	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	1	20	theme	great	185:189	arg1	challenge					191:199	a great challenge	183:199	a great challenge to clinical management and patients	183:235	Chronic and non-healing wounds pose a great challenge to clinical management and patients.					
35731847	0	21	theme	Cross-Linked	5:16	arg1	Alginate					18:25	Zn2+ Cross-Linked Alginate	0:25	Zn2+ Cross-Linked Alginate Carrying Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides	0:91	Zn2+ Cross-Linked Alginate Carrying Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides Provides Promising Treatment for Chronic Skin Wounds.					
35731847	3	22	theme	HSN	540:542	arg1	nanocomposites					552:565	these HSN@RL-QN15 nanocomposites	534:565	these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel	534:642	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	3	23	theme	zinc	586:589	arg1	gels					605:608	zinc alginate (ZA) gels	586:608	zinc alginate (ZA) gels	586:608	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	7	24	from	dressing	1441:1448	arg1	field					1457:1461	the field	1453:1461	the field of tissue regeneration	1453:1484	This peptide-based hydrogel provides a novel intervention for the treatment of chronic skin wounds and shows promise as a wound dressing in the field of tissue regeneration.					
35731847	3	25	theme	prohealing	450:459	arg1	RL-QN15					469:475	the prohealing peptide RL-QN15	446:475	the prohealing peptide RL-QN15	446:475	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	0	26	theme	Zn2+	0:3	arg1	Alginate					18:25	Zn2+ Cross-Linked Alginate	0:25	Zn2+ Cross-Linked Alginate Carrying Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides	0:91	Zn2+ Cross-Linked Alginate Carrying Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides Provides Promising Treatment for Chronic Skin Wounds.					
35731847	6	27	theme	biofilm-infected	1272:1287	arg1	wounds					1297:1302	biofilm-infected chronic wounds	1272:1302	methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice	1228:1310	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	3	28	theme	alginate	591:598	arg1	gels					605:608	zinc alginate (ZA) gels	586:608	zinc alginate (ZA) gels	586:608	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	2	29	theme	therapeutic	408:418	arg1	potential					420:428	their therapeutic potential	402:428	their therapeutic potential	402:428	Many studies have explored novel interventions against skin wounds, with bioactive peptides, nanoparticles, and hydrogels arousing considerable attention regarding their therapeutic potential.					
35731847	6	30	theme	cell	989:992	arg1	proliferation					994:1006	skin cell proliferation	984:1006	skin cell proliferation	984:1006	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	6	31	dep	aureus	1265:1270	arg1	wounds					1297:1302	biofilm-infected chronic wounds	1272:1302	methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice	1228:1310	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	7	32	theme	tissue	1466:1471	arg1	regeneration					1473:1484	tissue regeneration	1466:1484	tissue regeneration	1466:1484	This peptide-based hydrogel provides a novel intervention for the treatment of chronic skin wounds and shows promise as a wound dressing in the field of tissue regeneration.					
35731847	1	33	theme	clinical	204:211	arg1	management					213:222	clinical management	204:222	clinical management	204:222	Chronic and non-healing wounds pose a great challenge to clinical management and patients.					
35731847	6	34	theme	full-thickness	1197:1210	arg1	wounds					1217:1222	full-thickness skin wounds	1197:1222	full-thickness skin wounds	1197:1222	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	6	35	theme	skin	984:987	arg1	proliferation					994:1006	skin cell proliferation	984:1006	skin cell proliferation	984:1006	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	0	36	theme	Skin	134:137	arg1	Wounds					139:144	Chronic Skin Wounds	126:144	Chronic Skin Wounds	126:144	Zn2+ Cross-Linked Alginate Carrying Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides Provides Promising Treatment for Chronic Skin Wounds.					
35731847	2	37	theme	bioactive	311:319	arg1	peptides					321:328	bioactive peptides	311:328	bioactive peptides	311:328	Many studies have explored novel interventions against skin wounds, with bioactive peptides, nanoparticles, and hydrogels arousing considerable attention regarding their therapeutic potential.					
35731847	3	38	theme	silica	500:505	arg1	HSNs					522:525	HSNs	522:525	HSNs	522:525	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	3	38	theme	silica	500:505	arg1	nanoparticles					507:519	hollow silica nanoparticles	493:519	hollow silica nanoparticles (HSNs)	493:526	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	0	39	theme	Silica	43:48	arg1	Nanoparticles					50:62	Hollow Silica Nanoparticles	36:62	Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides	36:91	Zn2+ Cross-Linked Alginate Carrying Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides Provides Promising Treatment for Chronic Skin Wounds.					
35731847	6	40	theme	rapid	1175:1179	arg1	healing					1181:1187	the rapid healing	1171:1187	the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice	1171:1310	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	3	41	theme	peptide	461:467	arg1	RL-QN15					469:475	the prohealing peptide RL-QN15	446:475	the prohealing peptide RL-QN15	446:475	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	5	42	theme	broad-spectrum	850:863	arg1	activity					879:886	broad-spectrum antimicrobial activity	850:886	broad-spectrum antimicrobial activity	850:886	Results showed that the hydrogel had good porosity, hemocompatibility, biocompatibility, and broad-spectrum antimicrobial activity, with the slow release of loaded RL-QN15.					
35731847	0	43	theme	Hollow	36:41	arg1	Nanoparticles					50:62	Hollow Silica Nanoparticles	36:62	Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides	36:91	Zn2+ Cross-Linked Alginate Carrying Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides Provides Promising Treatment for Chronic Skin Wounds.					
35731847	3	44	theme	RL-QN15/ZA	624:633	arg1	hydrogel					635:642	HSN@RL-QN15/ZA hydrogel	620:642	HSN@RL-QN15/ZA hydrogel	620:642	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	5	45	theme	antimicrobial	865:877	arg1	activity					879:886	broad-spectrum antimicrobial activity	850:886	broad-spectrum antimicrobial activity	850:886	Results showed that the hydrogel had good porosity, hemocompatibility, biocompatibility, and broad-spectrum antimicrobial activity, with the slow release of loaded RL-QN15.					
35731847	5	46	theme	good	794:797	arg1	porosity					799:806	good porosity	794:806	good porosity	794:806	Results showed that the hydrogel had good porosity, hemocompatibility, biocompatibility, and broad-spectrum antimicrobial activity, with the slow release of loaded RL-QN15.					
35731847	2	47	theme	Many	238:241	arg1	studies					243:249	Many studies	238:249	Many studies	238:249	Many studies have explored novel interventions against skin wounds, with bioactive peptides, nanoparticles, and hydrogels arousing considerable attention regarding their therapeutic potential.					
35731847	5	48	theme	RL-QN15	921:927	arg1	release					903:909	the slow release	894:909	the slow release of loaded RL-QN15	894:927	Results showed that the hydrogel had good porosity, hemocompatibility, biocompatibility, and broad-spectrum antimicrobial activity, with the slow release of loaded RL-QN15.					
35731847	6	49	theme	accelerated	1091:1101	arg1	formation					1147:1155	accelerated re-epithelialization and granulation tissue formation	1091:1155	accelerated re-epithelialization and granulation tissue formation	1091:1155	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	3	50	theme	ZA	601:602	arg1	gels					605:608	zinc alginate (ZA) gels	586:608	zinc alginate (ZA) gels	586:608	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	4	51	theme	biological	666:675	arg1	properties					677:686	biological properties	666:686	biological properties	666:686	The characteristics, biological properties, and safety profiles of the hydrogel composites were then evaluated.					
35731847	2	52	with	interventions	271:283	arg1	hydrogels					350:358	hydrogels	350:358	hydrogels	350:358	Many studies have explored novel interventions against skin wounds, with bioactive peptides, nanoparticles, and hydrogels arousing considerable attention regarding their therapeutic potential.					
35731847	2	52	with	interventions	271:283	arg1	peptides					321:328	bioactive peptides	311:328	bioactive peptides	311:328	Many studies have explored novel interventions against skin wounds, with bioactive peptides, nanoparticles, and hydrogels arousing considerable attention regarding their therapeutic potential.					
35731847	2	52	with	interventions	271:283	arg1	nanoparticles					331:343	nanoparticles	331:343	nanoparticles	331:343	Many studies have explored novel interventions against skin wounds, with bioactive peptides, nanoparticles, and hydrogels arousing considerable attention regarding their therapeutic potential.					
35731847	7	53	theme	wound	1435:1439	arg1	dressing					1441:1448	a wound dressing	1433:1448	a wound dressing in the field of tissue regeneration	1433:1484	This peptide-based hydrogel provides a novel intervention for the treatment of chronic skin wounds and shows promise as a wound dressing in the field of tissue regeneration.					
35731847	7	53	theme	wound	1435:1439	arg1	promise					1422:1428	promise	1422:1428	promise	1422:1428	This peptide-based hydrogel provides a novel intervention for the treatment of chronic skin wounds and shows promise as a wound dressing in the field of tissue regeneration.					
35731847	3	54	theme	HSN	620:622	arg1	hydrogel					635:642	HSN@RL-QN15/ZA hydrogel	620:642	HSN@RL-QN15/ZA hydrogel	620:642	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	4	55	theme	composites	725:734	arg1	profiles					700:707	safety profiles	693:707	safety profiles	693:707	The characteristics, biological properties, and safety profiles of the hydrogel composites were then evaluated.					
35731847	4	55	theme	composites	725:734	arg1	characteristics					649:663	The characteristics	645:663	The characteristics	645:663	The characteristics, biological properties, and safety profiles of the hydrogel composites were then evaluated.					
35731847	4	55	theme	composites	725:734	arg1	properties					677:686	biological properties	666:686	biological properties	666:686	The characteristics, biological properties, and safety profiles of the hydrogel composites were then evaluated.					
35731847	4	56	theme	safety	693:698	arg1	profiles					700:707	safety profiles	693:707	safety profiles	693:707	The characteristics, biological properties, and safety profiles of the hydrogel composites were then evaluated.					
35731847	3	57	theme	RL-QN15	544:550	arg1	nanocomposites					552:565	these HSN@RL-QN15 nanocomposites	534:565	these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel	534:642	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	5	58	contain	had	790:792	arg2	biocompatibility					828:843	biocompatibility	828:843	biocompatibility	828:843	Results showed that the hydrogel had good porosity, hemocompatibility, biocompatibility, and broad-spectrum antimicrobial activity, with the slow release of loaded RL-QN15.					
35731847	5	58	contain	had	790:792	arg2	hemocompatibility					809:825	hemocompatibility	809:825	hemocompatibility	809:825	Results showed that the hydrogel had good porosity, hemocompatibility, biocompatibility, and broad-spectrum antimicrobial activity, with the slow release of loaded RL-QN15.					
35731847	5	58	contain	had	790:792	arg1	hydrogel					781:788	the hydrogel	777:788	the hydrogel	777:788	Results showed that the hydrogel had good porosity, hemocompatibility, biocompatibility, and broad-spectrum antimicrobial activity, with the slow release of loaded RL-QN15.					
35731847	5	58	contain	had	790:792	arg2	porosity					799:806	good porosity	794:806	good porosity	794:806	Results showed that the hydrogel had good porosity, hemocompatibility, biocompatibility, and broad-spectrum antimicrobial activity, with the slow release of loaded RL-QN15.					
35731847	5	58	contain	had	790:792	arg2	activity					879:886	broad-spectrum antimicrobial activity	850:886	broad-spectrum antimicrobial activity	850:886	Results showed that the hydrogel had good porosity, hemocompatibility, biocompatibility, and broad-spectrum antimicrobial activity, with the slow release of loaded RL-QN15.					
35731847	3	59	theme	@	623:623	arg1	hydrogel					635:642	HSN@RL-QN15/ZA hydrogel	620:642	HSN@RL-QN15/ZA hydrogel	620:642	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	1	60	theme	Chronic	147:153	arg1	wounds					171:176	Chronic and non-healing wounds	147:176	Chronic and non-healing wounds	147:176	Chronic and non-healing wounds pose a great challenge to clinical management and patients.					
35731847	7	61	theme	wounds	1405:1410	arg1	treatment					1379:1387	the treatment	1375:1387	the treatment of chronic skin wounds	1375:1410	This peptide-based hydrogel provides a novel intervention for the treatment of chronic skin wounds and shows promise as a wound dressing in the field of tissue regeneration.					
35731847	7	62	theme	novel	1352:1356	arg1	intervention					1358:1369	a novel intervention	1350:1369	a novel intervention	1350:1369	This peptide-based hydrogel provides a novel intervention for the treatment of chronic skin wounds and shows promise as a wound dressing in the field of tissue regeneration.					
35731847	0	63	theme	RL-QN15	76:82	arg1	Peptides					84:91	RL-QN15 Peptides	76:91	RL-QN15 Peptides	76:91	Zn2+ Cross-Linked Alginate Carrying Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides Provides Promising Treatment for Chronic Skin Wounds.					
35731847	6	64	theme	reduced	1065:1071	arg1	inflammation					1073:1084	reduced inflammation	1065:1084	reduced inflammation	1065:1084	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	2	65	theme	novel	265:269	arg1	interventions					271:283	novel interventions	265:283	novel interventions	265:283	Many studies have explored novel interventions against skin wounds, with bioactive peptides, nanoparticles, and hydrogels arousing considerable attention regarding their therapeutic potential.					
35731847	3	66	theme	hollow	493:498	arg1	HSNs					522:525	HSNs	522:525	HSNs	522:525	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	3	66	theme	hollow	493:498	arg1	nanoparticles					507:519	hollow silica nanoparticles	493:519	hollow silica nanoparticles (HSNs)	493:526	In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these HSN@RL-QN15 nanocomposites then combined with zinc alginate (ZA) gels to obtain HSN@RL-QN15/ZA hydrogel.					
35731847	5	67	theme	slow	898:901	arg1	release					903:909	the slow release	894:909	the slow release of loaded RL-QN15	894:927	Results showed that the hydrogel had good porosity, hemocompatibility, biocompatibility, and broad-spectrum antimicrobial activity, with the slow release of loaded RL-QN15.					
35731847	7	68	theme	chronic	1392:1398	arg1	wounds					1405:1410	chronic skin wounds	1392:1410	chronic skin wounds	1392:1410	This peptide-based hydrogel provides a novel intervention for the treatment of chronic skin wounds and shows promise as a wound dressing in the field of tissue regeneration.					
35731847	6	69	theme	regulated	1041:1049	arg1	angiogenesis					1051:1062	regulated angiogenesis	1041:1062	regulated angiogenesis	1041:1062	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	6	70	theme	aureus	1265:1270	arg1	healing					1181:1187	the rapid healing	1171:1187	the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice	1171:1310	Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant Staphylococcus aureus biofilm-infected chronic wounds in mice.					
35731847	1	71	theme	non-healing	159:169	arg1	wounds					171:176	Chronic and non-healing wounds	147:176	Chronic and non-healing wounds	147:176	Chronic and non-healing wounds pose a great challenge to clinical management and patients.					
36604067	6	0	theme	rich	1028:1031	arg1	structure					1040:1048	a rich porous structure	1026:1048	a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g	1026:1109	The obtained sample has a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g.					
36604067	7	1	theme	porous	1153:1158	arg1	structure					1160:1168	uniformly three-dimensional porous structure	1125:1168	uniformly three-dimensional porous structure	1125:1168	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	7	1	theme	porous	1153:1158	arg1	rigidity					1201:1208	the sample present preferable rigidity	1171:1208	the sample present preferable rigidity	1171:1208	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	1	2	theme	capture	194:200	arg1	technology					202:211	Developing affordable and effective carbon dioxide (CO2) capture technology	137:211	Developing affordable and effective carbon dioxide (CO2) capture technology	137:211	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	9	3	theme	CO2	1485:1487	arg1	process					1497:1503	the CO2 capture process	1481:1503	the CO2 capture process	1481:1503	This adsorbent has broad prospects in the CO2 capture process.					
36604067	1	4	theme	CO2	295:297	arg1	emissions					299:307	global CO2 emissions	288:307	global CO2 emissions	288:307	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	0	5	theme	dioxide	120:126	arg1	capture					128:134	carbon dioxide capture	113:134	carbon dioxide capture	113:134	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel as adsorbents for carbon dioxide capture.					
36604067	0	6	link	epichlorohydrin-cross-linked	48:75	arg1	aerogel					87:93	epichlorohydrin-cross-linked cellulose aerogel	48:93	epichlorohydrin-cross-linked cellulose aerogel	48:93	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel as adsorbents for carbon dioxide capture.					
36604067	0	6	link	epichlorohydrin-cross-linked	48:75	arg1	adsorbents					98:107	adsorbents	98:107	adsorbents for carbon dioxide capture	98:134	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel as adsorbents for carbon dioxide capture.					
36604067	7	7	theme	present	1182:1188	arg1	structure					1160:1168	uniformly three-dimensional porous structure	1125:1168	uniformly three-dimensional porous structure	1125:1168	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	7	7	theme	present	1182:1188	arg1	rigidity					1201:1208	the sample present preferable rigidity	1171:1208	the sample present preferable rigidity	1171:1208	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	1	8	theme	emissions	299:307	arg1	growth					278:283	the continued growth	264:283	the continued growth of global CO2 emissions	264:307	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	3	9	theme	modified	444:451	arg1	EBPCa					501:505	EBPCa	501:505	EBPCa	501:505	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	3	9	theme	modified	444:451	arg1	aerogel					492:498	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel	405:498	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa)	405:506	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	2	10	theme	cellulosic	341:350	arg1	materials					352:360	The low-cost and biodegradable cellulosic materials	310:360	The low-cost and biodegradable cellulosic materials	310:360	The low-cost and biodegradable cellulosic materials are developed into CO2 adsorbent recently.					
36604067	9	11	contain	has	1458:1460	arg2	prospects					1468:1476	broad prospects	1462:1476	broad prospects	1462:1476	This adsorbent has broad prospects in the CO2 capture process.					
36604067	9	11	contain	has	1458:1460	arg1	adsorbent					1448:1456	adsorbent	1448:1456	adsorbent	1448:1456	This adsorbent has broad prospects in the CO2 capture process.					
36604067	3	12	theme	epichlorohydrin-cross-linked	453:480	arg1	EBPCa					501:505	EBPCa	501:505	EBPCa	501:505	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	3	12	theme	epichlorohydrin-cross-linked	453:480	arg1	aerogel					492:498	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel	405:498	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa)	405:506	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	4	13	dep	Fourier	695:701	arg1	transform					703:711	transform	703:711	transform infrared spectroscopy	703:733	The Fourier transform infrared spectroscopy confirmed that the cellulose aerogel was successfully modified by EB-PEI.					
36604067	5	14	theme	X-ray	813:817	arg1	analyses					846:853	The X-ray photoelectron spectroscopy analyses	809:853	The X-ray photoelectron spectroscopy analyses	809:853	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	7	15	contain	carry	1266:1270	arg2	capacity					1223:1230	capacity	1223:1230	capacity	1223:1230	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	7	15	contain	carry	1266:1270	arg2	weight					1276:1281	the weight	1272:1281	the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg)	1272:1342	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	7	15	contain	carry	1266:1270	arg1	1 g					1239:1241	1 g	1239:1241	1 g of sample	1239:1251	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	2	16	theme	biodegradable	327:339	arg1	materials					352:360	The low-cost and biodegradable cellulosic materials	310:360	The low-cost and biodegradable cellulosic materials	310:360	The low-cost and biodegradable cellulosic materials are developed into CO2 adsorbent recently.					
36604067	4	17	mod	modified	789:796	arg3	EB-PEI					801:806	EB-PEI	801:806	EB-PEI	801:806	The Fourier transform infrared spectroscopy confirmed that the cellulose aerogel was successfully modified by EB-PEI.					
36604067	4	17	mod	modified	789:796	arg1	aerogel					764:770	the cellulose aerogel	750:770	the cellulose aerogel	750:770	The Fourier transform infrared spectroscopy confirmed that the cellulose aerogel was successfully modified by EB-PEI.					
36604067	5	18	theme	EB-PEI	963:968	arg1	chlorine					923:930	the chlorine	919:930	the chlorine of ECH	919:937	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	18	theme	EB-PEI	963:968	arg1	EB-PEI					963:968	EB-PEI	963:968	EB-PEI	963:968	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	18	theme	EB-PEI	963:968	arg1	groups					953:958	the amino groups	943:958	the amino groups of EB-PEI	943:968	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	18	theme	EB-PEI	963:968	arg1	ECH					935:937	ECH	935:937	ECH	935:937	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	3	19	theme	Epoxy-functionalized	405:424	arg1	EBPCa					501:505	EBPCa	501:505	EBPCa	501:505	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	3	19	theme	Epoxy-functionalized	405:424	arg1	aerogel					492:498	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel	405:498	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa)	405:506	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	5	20	attach	presence	869:876	arg2	1s					883:884	N 1s	881:884	N 1s	881:884	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	20	attach	presence	869:876	arg2	Cl					890:891	Cl 2p	890:894	Cl 2p	890:894	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	20	attach	presence	869:876	arg1	meaning					906:912	EBPCa, meaning	899:912	meaning	906:912	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	21	from	presence	869:876	arg1	meaning					906:912	EBPCa, meaning	899:912	meaning	906:912	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	3	22	theme	alkaline	529:536	arg1	solution					548:555	alkaline cellulose solution	529:555	alkaline cellulose solution	529:555	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	6	23	contain	has	1022:1024	arg2	structure					1040:1048	a rich porous structure	1026:1048	a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g	1026:1109	The obtained sample has a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g.					
36604067	6	23	contain	has	1022:1024	arg1	sample					1015:1020	The obtained sample	1002:1020	The obtained sample	1002:1020	The obtained sample has a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g.					
36604067	3	24	theme	polyethyleneimine	426:442	arg1	EBPCa					501:505	EBPCa	501:505	EBPCa	501:505	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	3	24	theme	polyethyleneimine	426:442	arg1	aerogel					492:498	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel	405:498	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa)	405:506	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	6	25	with	structure	1040:1048	arg1	area					1074:1077	a specific surface area	1055:1077	a specific surface area in the range of 97.5-149.5 m2/g	1055:1109	The obtained sample has a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g.					
36604067	7	26	theme	preferable	1190:1199	arg1	structure					1160:1168	uniformly three-dimensional porous structure	1125:1168	uniformly three-dimensional porous structure	1125:1168	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	7	26	theme	preferable	1190:1199	arg1	rigidity					1201:1208	the sample present preferable rigidity	1171:1208	the sample present preferable rigidity	1171:1208	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	2	27	theme	low-cost	314:321	arg1	materials					352:360	The low-cost and biodegradable cellulosic materials	310:360	The low-cost and biodegradable cellulosic materials	310:360	The low-cost and biodegradable cellulosic materials are developed into CO2 adsorbent recently.					
36604067	5	28	theme	Cl	890:891	arg1	presence					869:876	the presence	865:876	the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface	865:999	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	29	theme	ECH	935:937	arg1	chlorine					923:930	the chlorine	919:930	the chlorine of ECH	919:937	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	29	theme	ECH	935:937	arg1	EB-PEI					963:968	EB-PEI	963:968	EB-PEI	963:968	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	29	theme	ECH	935:937	arg1	groups					953:958	the amino groups	943:958	the amino groups of EB-PEI	943:968	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	29	theme	ECH	935:937	arg1	ECH					935:937	ECH	935:937	ECH	935:937	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	6	30	theme	obtained	1006:1013	arg1	sample					1015:1020	The obtained sample	1002:1020	The obtained sample	1002:1020	The obtained sample has a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g.					
36604067	1	31	theme	substantial	227:237	arg1	attention					247:255	substantial intense attention	227:255	substantial intense attention	227:255	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	0	32	theme	Epoxy-functionalized	0:19	arg1	polyethyleneimine					21:37	Epoxy-functionalized polyethyleneimine	0:37	Epoxy-functionalized polyethyleneimine	0:37	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel as adsorbents for carbon dioxide capture.					
36604067	1	33	theme	Developing	137:146	arg1	dioxide					180:186	Developing affordable and effective carbon dioxide	137:186	Developing affordable and effective carbon dioxide (CO2) capture technology	137:211	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	1	33	theme	Developing	137:146	arg1	CO2					189:191	CO2	189:191	CO2	189:191	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	5	34	theme	1s	883:884	arg1	presence					869:876	the presence	865:876	the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface	865:999	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	1	35	theme	intense	239:245	arg1	attention					247:255	substantial intense attention	227:255	substantial intense attention	227:255	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	0	36	theme	epichlorohydrin-cross-linked	48:75	arg1	aerogel					87:93	epichlorohydrin-cross-linked cellulose aerogel	48:93	epichlorohydrin-cross-linked cellulose aerogel	48:93	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel as adsorbents for carbon dioxide capture.					
36604067	0	36	theme	epichlorohydrin-cross-linked	48:75	arg1	adsorbents					98:107	adsorbents	98:107	adsorbents for carbon dioxide capture	98:134	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel as adsorbents for carbon dioxide capture.					
36604067	6	37	from	area	1074:1077	arg1	range					1086:1090	the range	1082:1090	the range of 97.5-149.5 m2/g	1082:1109	The obtained sample has a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g.					
36604067	1	38	theme	affordable	148:157	arg1	dioxide					180:186	Developing affordable and effective carbon dioxide	137:186	Developing affordable and effective carbon dioxide (CO2) capture technology	137:211	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	1	38	theme	affordable	148:157	arg1	CO2					189:191	CO2	189:191	CO2	189:191	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	5	39	theme	amino	947:951	arg1	EB-PEI					963:968	EB-PEI	963:968	EB-PEI	963:968	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	39	theme	amino	947:951	arg1	groups					953:958	the amino groups	943:958	the amino groups of EB-PEI	943:968	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	39	theme	amino	947:951	arg1	ECH					935:937	ECH	935:937	ECH	935:937	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	40	dep	meaning	906:912	arg1	exist					970:974	exist	970:974	exist in the cellulose surface	970:999	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	7	41	theme	flask	1307:1311	arg1	weight					1276:1281	the weight	1272:1281	the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg)	1272:1342	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	6	42	theme	surface	1066:1072	arg1	area					1074:1077	a specific surface area	1055:1077	a specific surface area in the range of 97.5-149.5 m2/g	1055:1109	The obtained sample has a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g.					
36604067	7	43	theme	3000 ml	1288:1294	arg1	flask					1307:1311	a 3000 ml Erlenmeyer flask	1286:1311	a 3000 ml Erlenmeyer flask filled with water (total 4 kg)	1286:1342	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	3	44	theme	freezing-thawing	645:660	arg1	processes					662:670	the freezing-thawing processes	641:670	the freezing-thawing processes	641:670	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	3	45	theme	cellulose	538:546	arg1	solution					548:555	alkaline cellulose solution	529:555	alkaline cellulose solution	529:555	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	3	46	theme	epoxy-functionalized	558:577	arg1	polyethyleneimine					579:595	epoxy-functionalized polyethyleneimine	558:595	epoxy-functionalized polyethyleneimine (EB-PEI)	558:604	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	3	46	theme	epoxy-functionalized	558:577	arg1	EB-PEI					598:603	EB-PEI	598:603	EB-PEI	598:603	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	0	47	theme	cellulose	77:85	arg1	aerogel					87:93	epichlorohydrin-cross-linked cellulose aerogel	48:93	epichlorohydrin-cross-linked cellulose aerogel	48:93	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel as adsorbents for carbon dioxide capture.					
36604067	0	47	theme	cellulose	77:85	arg1	adsorbents					98:107	adsorbents	98:107	adsorbents for carbon dioxide capture	98:134	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel as adsorbents for carbon dioxide capture.					
36604067	6	48	theme	specific	1057:1064	arg1	area					1074:1077	a specific surface area	1055:1077	a specific surface area in the range of 97.5-149.5 m2/g	1055:1109	The obtained sample has a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g.					
36604067	5	49	theme	N	881:881	arg1	1s					883:884	N 1s	881:884	N 1s	881:884	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	6	50	theme	97.5-149.5 m2/g	1095:1109	arg1	range					1086:1090	the range	1082:1090	the range of 97.5-149.5 m2/g	1082:1109	The obtained sample has a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g.					
36604067	3	51	theme	cellulose	482:490	arg1	EBPCa					501:505	EBPCa	501:505	EBPCa	501:505	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	3	51	theme	cellulose	482:490	arg1	aerogel					492:498	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel	405:498	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa)	405:506	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	9	52	theme	broad	1462:1466	arg1	prospects					1468:1476	broad prospects	1462:1476	broad prospects	1462:1476	This adsorbent has broad prospects in the CO2 capture process.					
36604067	7	53	theme	Erlenmeyer	1296:1305	arg1	flask					1307:1311	a 3000 ml Erlenmeyer flask	1286:1311	a 3000 ml Erlenmeyer flask filled with water (total 4 kg)	1286:1342	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	7	54	theme	sample	1246:1251	arg1	1 g					1239:1241	1 g	1239:1241	1 g of sample	1239:1251	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	5	55	theme	cellulose	983:991	arg1	surface					993:999	the cellulose surface	979:999	the cellulose surface	979:999	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	5	56	theme	photoelectron	819:831	arg1	analyses					846:853	The X-ray photoelectron spectroscopy analyses	809:853	The X-ray photoelectron spectroscopy analyses	809:853	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	4	57	dep	transform	703:711	arg1	infrared					713:720	infrared	713:720	transform infrared spectroscopy	703:733	The Fourier transform infrared spectroscopy confirmed that the cellulose aerogel was successfully modified by EB-PEI.					
36604067	1	58	theme	effective	163:171	arg1	dioxide					180:186	Developing affordable and effective carbon dioxide	137:186	Developing affordable and effective carbon dioxide (CO2) capture technology	137:211	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	1	58	theme	effective	163:171	arg1	CO2					189:191	CO2	189:191	CO2	189:191	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	7	59	theme	sample	1175:1180	arg1	structure					1160:1168	uniformly three-dimensional porous structure	1125:1168	uniformly three-dimensional porous structure	1125:1168	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	7	59	theme	sample	1175:1180	arg1	rigidity					1201:1208	the sample present preferable rigidity	1171:1208	the sample present preferable rigidity	1171:1208	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	5	60	theme	spectroscopy	833:844	arg1	analyses					846:853	The X-ray photoelectron spectroscopy analyses	809:853	The X-ray photoelectron spectroscopy analyses	809:853	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	1	61	theme	carbon	173:178	arg1	dioxide					180:186	Developing affordable and effective carbon dioxide	137:186	Developing affordable and effective carbon dioxide (CO2) capture technology	137:211	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	1	61	theme	carbon	173:178	arg1	CO2					189:191	CO2	189:191	CO2	189:191	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	7	62	theme	three-dimensional	1135:1151	arg1	structure					1160:1168	uniformly three-dimensional porous structure	1125:1168	uniformly three-dimensional porous structure	1125:1168	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	7	62	theme	three-dimensional	1135:1151	arg1	rigidity					1201:1208	the sample present preferable rigidity	1171:1208	the sample present preferable rigidity	1171:1208	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	1	63	theme	dioxide	180:186	arg1	technology					202:211	Developing affordable and effective carbon dioxide (CO2) capture technology	137:211	Developing affordable and effective carbon dioxide (CO2) capture technology	137:211	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	1	64	theme	continued	268:276	arg1	growth					278:283	the continued growth	264:283	the continued growth of global CO2 emissions	264:307	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36604067	3	65	link	epichlorohydrin-cross-linked	453:480	arg1	EBPCa					501:505	EBPCa	501:505	EBPCa	501:505	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	3	65	link	epichlorohydrin-cross-linked	453:480	arg1	aerogel					492:498	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel	405:498	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa)	405:506	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel (EBPCa) was synthesized from alkaline cellulose solution, epoxy-functionalized polyethyleneimine (EB-PEI), and epichlorohydrin (ECH) through the freezing-thawing processes and freeze-drying.					
36604067	8	66	theme	adsorption	1368:1377	arg1	performance					1379:1389	good adsorption performance	1363:1389	good adsorption performance	1363:1389	The sample showed good adsorption performance, with a maximum adsorption capacity of 6.45 mmol/g.					
36604067	8	67	theme	6.45 mmol/g	1430:1440	arg1	capacity					1418:1425	a maximum adsorption capacity	1397:1425	a maximum adsorption capacity of 6.45 mmol/g	1397:1440	The sample showed good adsorption performance, with a maximum adsorption capacity of 6.45 mmol/g.					
36604067	2	68	theme	adsorbent	385:393	arg1	CO2					381:383	CO2 adsorbent	381:393	CO2 adsorbent	381:393	The low-cost and biodegradable cellulosic materials are developed into CO2 adsorbent recently.					
36604067	0	69	theme	carbon	113:118	arg1	dioxide					120:126	carbon dioxide	113:126	carbon dioxide capture	113:134	Epoxy-functionalized polyethyleneimine modified epichlorohydrin-cross-linked cellulose aerogel as adsorbents for carbon dioxide capture.					
36604067	8	70	theme	good	1363:1366	arg1	performance					1379:1389	good adsorption performance	1363:1389	good adsorption performance	1363:1389	The sample showed good adsorption performance, with a maximum adsorption capacity of 6.45 mmol/g.					
36604067	8	71	theme	adsorption	1407:1416	arg1	capacity					1418:1425	a maximum adsorption capacity	1397:1425	a maximum adsorption capacity of 6.45 mmol/g	1397:1440	The sample showed good adsorption performance, with a maximum adsorption capacity of 6.45 mmol/g.					
36604067	7	72	theme	total	1332:1336	arg1	water					1325:1329	water	1325:1329	water (total 4 kg)	1325:1342	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	7	72	theme	total	1332:1336	arg1	4 kg					1338:1341	total 4 kg	1332:1341	total 4 kg	1332:1341	Owing to its uniformly three-dimensional porous structure, the sample present preferable rigidity and carrying capacity, which 1 g of sample could easily carry the weight of a 3000 ml Erlenmeyer flask filled with water (total 4 kg).					
36604067	9	73	theme	capture	1489:1495	arg1	process					1497:1503	the CO2 capture process	1481:1503	the CO2 capture process	1481:1503	This adsorbent has broad prospects in the CO2 capture process.					
36604067	5	74	theme	EBPCa	899:903	arg1	meaning					906:912	EBPCa, meaning	899:912	meaning	906:912	The X-ray photoelectron spectroscopy analyses confirmed the presence of N 1s and Cl 2p in EBPCa, meaning that the chlorine of ECH and the amino groups of EB-PEI exist in the cellulose surface.					
36604067	6	75	theme	porous	1033:1038	arg1	structure					1040:1048	a rich porous structure	1026:1048	a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g	1026:1109	The obtained sample has a rich porous structure with a specific surface area in the range of 97.5-149.5 m2/g.					
36604067	4	76	theme	cellulose	754:762	arg1	aerogel					764:770	the cellulose aerogel	750:770	the cellulose aerogel	750:770	The Fourier transform infrared spectroscopy confirmed that the cellulose aerogel was successfully modified by EB-PEI.					
36604067	8	77	theme	maximum	1399:1405	arg1	capacity					1418:1425	a maximum adsorption capacity	1397:1425	a maximum adsorption capacity of 6.45 mmol/g	1397:1440	The sample showed good adsorption performance, with a maximum adsorption capacity of 6.45 mmol/g.					
36604067	1	78	theme	global	288:293	arg1	emissions					299:307	global CO2 emissions	288:307	global CO2 emissions	288:307	Developing affordable and effective carbon dioxide (CO2) capture technology has attracted substantial intense attention due to the continued growth of global CO2 emissions.					
36934557	0	0	theme	PLGA	29:32	arg1	nanoparticles					34:46	PLGA nanoparticles	29:46	PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds	29:136	Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds.					
36934557	6	1	theme	In	922:923	arg1	studies					931:937	In vitro studies	922:937	In vitro studies	922:937	In vitro studies revealed that the HBD-2 COL-CS scaffold was biocompatible and accelerated cell migration and angiogenesis.					
36934557	4	2	theme	Poly	591:594	arg1	nanoparticle					629:640	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle	554:640	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW	554:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	10	3	theme	collagen	1534:1541	arg1	synthesis					1543:1551	collagen synthesis	1534:1551	collagen synthesis	1534:1551	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	1	4	from	mortality	221:229	arg1	patients					257:264	diabetic patients	248:264	diabetic patients	248:264	Diabetic wound (DW) is the most devastating complication resulting in significant mortality and morbidity in diabetic patients.					
36934557	2	5	theme	DW	293:294	arg1	care					296:299	DW care	293:299	DW care	293:299	The standard treatment of DW care fails to address the prerequisites of treating DW owing to its multifactorial pathophysiology.					
36934557	9	6	theme	HBD-2	1403:1407	arg1	COL-CS					1409:1414	HBD-2 COL-CS	1403:1414	HBD-2 COL-CS treated group	1403:1428	The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group.					
36934557	5	7	theme	crosslinked	787:797	arg1	scaffold					799:806	the developed biodegradable crosslinked scaffold	759:806	the developed biodegradable crosslinked scaffold	759:806	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	10	8	theme	PLGA	1528:1531	arg1	effects					1517:1523	the synergistic effects	1501:1523	the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release)	1501:1798	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	0	9	theme	Human	0:4	arg1	defensin-2					11:20	Human beta defensin-2	0:20	Human beta defensin-2	0:20	Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds.					
36934557	5	10	theme	drug	874:877	arg1	release					879:885	sustained drug release	864:885	sustained drug release	864:885	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	8	11	theme	COL-CS	1200:1205	arg1	group					1215:1219	the HBD-2 COL-CS treated group	1190:1219	the HBD-2 COL-CS treated group	1190:1219	The in vivo studies revealed that the HBD-2 COL-CS treated group accelerated healing compared to those in COL-CS and control groups.					
36934557	4	12	theme	PLGA	623:626	arg1	nanoparticle					629:640	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle	554:640	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW	554:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	0	13	theme	wounds	131:136	arg1	management					108:117	the management	104:117	the management of diabetic wounds	104:136	Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds.					
36934557	10	14	theme	wound	1723:1727	arg1	healer					1729:1734	established wound healer	1711:1734	established wound healer	1711:1734	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	6	15	dep	In	922:923	arg1	vitro					925:929	vitro	925:929	vitro	925:929	In vitro studies revealed that the HBD-2 COL-CS scaffold was biocompatible and accelerated cell migration and angiogenesis.					
36934557	10	16	theme	CS	1756:1757	arg1	effects					1517:1523	the synergistic effects	1501:1523	the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release)	1501:1798	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	6	17	theme	HBD-2	957:961	arg1	biocompatible					983:995	biocompatible	983:995	biocompatible	983:995	In vitro studies revealed that the HBD-2 COL-CS scaffold was biocompatible and accelerated cell migration and angiogenesis.					
36934557	6	17	theme	HBD-2	957:961	arg1	scaffold					970:977	the HBD-2 COL-CS scaffold	953:977	the HBD-2 COL-CS scaffold	953:977	In vitro studies revealed that the HBD-2 COL-CS scaffold was biocompatible and accelerated cell migration and angiogenesis.					
36934557	10	18	theme	angiogenic	1581:1590	arg1	effect					1592:1597	positive angiogenic effect	1572:1597	positive angiogenic effect	1572:1597	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	7	19	theme	significant	1079:1089	arg1	activity					1105:1112	significant antimicrobial activity	1079:1112	significant antimicrobial activity	1079:1112	The HBD-2 COL-CS scaffold showed significant antimicrobial activity in S. aureus, E. coli, and P. aeruginosa.					
36934557	5	20	link	crosslinked	787:797	arg1	scaffold					799:806	the developed biodegradable crosslinked scaffold	759:806	the developed biodegradable crosslinked scaffold	759:806	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	10	21	from	healing	1447:1453	arg1	group					1479:1483	HBD-2 COL-CS treated group	1458:1483	HBD-2 COL-CS treated group	1458:1483	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	2	22	theme	standard	271:278	arg1	treatment					280:288	The standard treatment	267:288	The standard treatment of DW care	267:299	The standard treatment of DW care fails to address the prerequisites of treating DW owing to its multifactorial pathophysiology.					
36934557	1	23	from	morbidity	235:243	arg1	patients					257:264	diabetic patients	248:264	diabetic patients	248:264	Diabetic wound (DW) is the most devastating complication resulting in significant mortality and morbidity in diabetic patients.					
36934557	10	24	theme	established	1711:1721	arg1	healer					1729:1734	established wound healer	1711:1734	established wound healer	1711:1734	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	25	dep	COL	1706:1708	arg1	healer					1729:1734	established wound healer	1711:1734	established wound healer	1711:1734	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	25	dep	COL	1706:1708	arg1	stabilizer					1740:1749	stabilizer	1740:1749	stabilizer	1740:1749	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	0	26	theme	collagen-chitosan	63:79	arg1	scaffold					91:98	collagen-chitosan composite scaffold	63:98	collagen-chitosan composite scaffold for the management of diabetic wounds	63:136	Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds.					
36934557	9	27	theme	ELISA	1293:1297	arg1	results					1299:1305	The ELISA results	1289:1305	The ELISA results	1289:1305	The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group.					
36934557	10	28	theme	antibacterial	1760:1772	arg1	CS					1756:1757	CS	1756:1757	CS (antibacterial, controlled drug release)	1756:1798	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	28	theme	antibacterial	1760:1772	arg1	release					1791:1797	antibacterial, controlled drug release	1760:1797	antibacterial, controlled drug release	1760:1797	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	5	29	theme	non-crosslinked	896:910	arg1	scaffold					912:919	the non-crosslinked scaffold	892:919	the non-crosslinked scaffold	892:919	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	5	30	theme	developed	763:771	arg1	scaffold					799:806	the developed biodegradable crosslinked scaffold	759:806	the developed biodegradable crosslinked scaffold	759:806	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	9	31	theme	significant	1319:1329	arg1	decrease					1331:1338	a significant decrease	1317:1338	a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO	1317:1372	The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group.					
36934557	10	32	theme	accelerated	1435:1445	arg1	due					1494:1496	due	1494:1496	due	1494:1496	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	32	theme	accelerated	1435:1445	arg1	healing					1447:1453	The accelerated healing	1431:1453	The accelerated healing in HBD-2 COL-CS treated group	1431:1483	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	4	33	theme	DW	735:736	arg1	healing					724:730	the accelerated healing	708:730	the accelerated healing of DW	708:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	5	34	theme	matrix	822:827	arg1	degradation					829:839	low matrix degradation	818:839	low matrix degradation	818:839	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	4	35	theme	Human	554:558	arg1	nanoparticle					629:640	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle	554:640	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW	554:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	10	36	theme	angiogenic	1651:1660	arg1	effect					1662:1667	positive angiogenic effect	1642:1667	positive angiogenic effect	1642:1667	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	3	37	theme	single	421:426	arg1	strategy					438:445	a single treatment strategy	419:445	a single treatment strategy to handle all the loopholes	419:473	Henceforth, developing a single treatment strategy to handle all the loopholes may effectively manage DW.					
36934557	4	38	theme	defensin-2	565:574	arg1	nanoparticle					629:640	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle	554:640	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW	554:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	10	39	theme	COL-CS	1464:1469	arg1	group					1479:1483	HBD-2 COL-CS treated group	1458:1483	HBD-2 COL-CS treated group	1458:1483	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	9	40	theme	COL-CS	1409:1414	arg1	group					1424:1428	HBD-2 COL-CS treated group	1403:1428	HBD-2 COL-CS treated group	1403:1428	The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group.					
36934557	9	41	from	IL-10	1394:1398	arg1	group					1424:1428	HBD-2 COL-CS treated group	1403:1428	HBD-2 COL-CS treated group	1403:1428	The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group.					
36934557	4	42	theme	study	531:535	arg1	objective					506:514	The objective	502:514	The objective of the current study	502:535	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	5	43	link	non-crosslinked	896:910	arg1	scaffold					912:919	the non-crosslinked scaffold	892:919	the non-crosslinked scaffold	892:919	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	9	44	from	increase	1382:1389	arg1	IL-10					1394:1398	IL-10	1394:1398	IL-10 in HBD-2 COL-CS treated group	1394:1428	The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group.					
36934557	2	45	theme	multifactorial	364:377	arg1	pathophysiology					379:393	its multifactorial pathophysiology	360:393	its multifactorial pathophysiology	360:393	The standard treatment of DW care fails to address the prerequisites of treating DW owing to its multifactorial pathophysiology.					
36934557	9	46	theme	treated	1416:1422	arg1	group					1424:1428	HBD-2 COL-CS treated group	1403:1428	HBD-2 COL-CS treated group	1403:1428	The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group.					
36934557	10	47	theme	synergistic	1505:1515	arg1	effects					1517:1523	the synergistic effects	1501:1523	the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release)	1501:1798	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	8	48	theme	in	1160:1161	arg1	studies					1168:1174	The in vivo studies	1156:1174	The in vivo studies	1156:1174	The in vivo studies revealed that the HBD-2 COL-CS treated group accelerated healing compared to those in COL-CS and control groups.					
36934557	4	49	theme	HBD-2	577:581	arg1	nanoparticle					629:640	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle	554:640	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW	554:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	2	50	theme	care	296:299	arg1	treatment					280:288	The standard treatment	267:288	The standard treatment of DW care	267:299	The standard treatment of DW care fails to address the prerequisites of treating DW owing to its multifactorial pathophysiology.					
36934557	4	51	theme	loaded	584:589	arg1	Poly					591:594	loaded Poly	584:594	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW	554:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	4	51	theme	loaded	584:589	arg1	acid					616:619	lactic-co-glycolic acid	597:619	lactic-co-glycolic acid	597:619	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	10	52	theme	COL	1706:1708	arg1	effects					1517:1523	the synergistic effects	1501:1523	the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release)	1501:1798	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	5	53	theme	sustained	864:872	arg1	release					879:885	sustained drug release	864:885	sustained drug release	864:885	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	10	54	dep	antibacterial	1760:1772	arg1	controlled					1775:1784	controlled	1775:1784	controlled	1775:1784	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	55	theme	positive	1572:1579	arg1	effect					1592:1597	positive angiogenic effect	1572:1597	positive angiogenic effect	1572:1597	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	1	56	theme	devastating	171:181	arg1	complication					183:194	the most devastating complication	162:194	the most devastating complication resulting in significant mortality and morbidity in diabetic patients	162:264	Diabetic wound (DW) is the most devastating complication resulting in significant mortality and morbidity in diabetic patients.					
36934557	1	56	theme	devastating	171:181	arg1	wound					148:152	Diabetic wound	139:152	Diabetic wound (DW)	139:157	Diabetic wound (DW) is the most devastating complication resulting in significant mortality and morbidity in diabetic patients.					
36934557	8	57	theme	HBD-2	1194:1198	arg1	group					1215:1219	the HBD-2 COL-CS treated group	1190:1219	the HBD-2 COL-CS treated group	1190:1219	The in vivo studies revealed that the HBD-2 COL-CS treated group accelerated healing compared to those in COL-CS and control groups.					
36934557	4	58	theme	collagen/chitosan	657:673	arg1	scaffolds					694:702	collagen/chitosan (COL-CS) composite scaffolds	657:702	collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW	657:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	5	59	theme	optimum	842:848	arg1	porosity					850:857	optimum porosity	842:857	optimum porosity	842:857	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	9	60	from	decrease	1331:1338	arg1	NAG					1362:1364	NAG	1362:1364	NAG	1362:1364	The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group.					
36934557	9	60	from	decrease	1331:1338	arg1	NO					1371:1372	NO	1371:1372	NO	1371:1372	The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group.					
36934557	9	60	from	decrease	1331:1338	arg1	MPO					1357:1359	MPO	1357:1359	MPO	1357:1359	The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group.					
36934557	9	60	from	decrease	1331:1338	arg1	TNF-α					1350:1354	TNF-α	1350:1354	TNF-α	1350:1354	The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group.					
36934557	9	60	from	decrease	1331:1338	arg1	MMP-9					1343:1347	MMP-9	1343:1347	MMP-9	1343:1347	The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group.					
36934557	8	61	theme	control	1273:1279	arg1	groups					1281:1286	control groups	1273:1286	control groups	1273:1286	The in vivo studies revealed that the HBD-2 COL-CS treated group accelerated healing compared to those in COL-CS and control groups.					
36934557	7	62	theme	antimicrobial	1091:1103	arg1	activity					1105:1112	significant antimicrobial activity	1079:1112	significant antimicrobial activity	1079:1112	The HBD-2 COL-CS scaffold showed significant antimicrobial activity in S. aureus, E. coli, and P. aeruginosa.					
36934557	1	63	theme	significant	209:219	arg1	mortality					221:229	significant mortality	209:229	significant mortality	209:229	Diabetic wound (DW) is the most devastating complication resulting in significant mortality and morbidity in diabetic patients.					
36934557	6	64	theme	COL-CS	963:968	arg1	biocompatible					983:995	biocompatible	983:995	biocompatible	983:995	In vitro studies revealed that the HBD-2 COL-CS scaffold was biocompatible and accelerated cell migration and angiogenesis.					
36934557	6	64	theme	COL-CS	963:968	arg1	scaffold					970:977	the HBD-2 COL-CS scaffold	953:977	the HBD-2 COL-CS scaffold	953:977	In vitro studies revealed that the HBD-2 COL-CS scaffold was biocompatible and accelerated cell migration and angiogenesis.					
36934557	7	65	theme	HBD-2	1050:1054	arg1	scaffold					1063:1070	The HBD-2 COL-CS scaffold	1046:1070	The HBD-2 COL-CS scaffold	1046:1070	The HBD-2 COL-CS scaffold showed significant antimicrobial activity in S. aureus, E. coli, and P. aeruginosa.					
36934557	5	66	contain	possesses	808:816	arg1	scaffold					799:806	the developed biodegradable crosslinked scaffold	759:806	the developed biodegradable crosslinked scaffold	759:806	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	5	66	contain	possesses	808:816	arg2	release					879:885	sustained drug release	864:885	sustained drug release	864:885	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	5	66	contain	possesses	808:816	arg2	degradation					829:839	low matrix degradation	818:839	low matrix degradation	818:839	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	5	66	contain	possesses	808:816	arg2	porosity					850:857	optimum porosity	842:857	optimum porosity	842:857	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	7	67	theme	COL-CS	1056:1061	arg1	scaffold					1063:1070	The HBD-2 COL-CS scaffold	1046:1070	The HBD-2 COL-CS scaffold	1046:1070	The HBD-2 COL-CS scaffold showed significant antimicrobial activity in S. aureus, E. coli, and P. aeruginosa.					
36934557	10	68	theme	drug	1786:1789	arg1	CS					1756:1757	CS	1756:1757	CS (antibacterial, controlled drug release)	1756:1798	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	68	theme	drug	1786:1789	arg1	release					1791:1797	antibacterial, controlled drug release	1760:1797	antibacterial, controlled drug release	1760:1797	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	1	69	theme	Diabetic	139:146	arg1	DW					155:156	DW	155:156	DW	155:156	Diabetic wound (DW) is the most devastating complication resulting in significant mortality and morbidity in diabetic patients.					
36934557	1	69	theme	Diabetic	139:146	arg1	wound					148:152	Diabetic wound	139:152	Diabetic wound (DW)	139:157	Diabetic wound (DW) is the most devastating complication resulting in significant mortality and morbidity in diabetic patients.					
36934557	1	69	theme	Diabetic	139:146	arg1	complication					183:194	the most devastating complication	162:194	the most devastating complication resulting in significant mortality and morbidity in diabetic patients	162:264	Diabetic wound (DW) is the most devastating complication resulting in significant mortality and morbidity in diabetic patients.					
36934557	4	70	theme	accelerated	712:722	arg1	healing					724:730	the accelerated healing	708:730	the accelerated healing of DW	708:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	0	71	theme	composite	81:89	arg1	scaffold					91:98	collagen-chitosan composite scaffold	63:98	collagen-chitosan composite scaffold for the management of diabetic wounds	63:136	Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds.					
36934557	8	72	theme	treated	1207:1213	arg1	group					1215:1219	the HBD-2 COL-CS treated group	1190:1219	the HBD-2 COL-CS treated group	1190:1219	The in vivo studies revealed that the HBD-2 COL-CS treated group accelerated healing compared to those in COL-CS and control groups.					
36934557	4	73	theme	COL-CS	676:681	arg1	scaffolds					694:702	collagen/chitosan (COL-CS) composite scaffolds	657:702	collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW	657:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	10	74	dep	PLGA	1528:1531	arg1	effect					1592:1597	positive angiogenic effect	1572:1597	positive angiogenic effect	1572:1597	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	74	dep	PLGA	1528:1531	arg1	deposition					1557:1566	deposition	1557:1566	deposition	1557:1566	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	74	dep	PLGA	1528:1531	arg1	synthesis					1543:1551	collagen synthesis	1534:1551	collagen synthesis	1534:1551	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	4	75	theme	current	523:529	arg1	study					531:535	the current study	519:535	the current study	519:535	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	4	76	theme	composite	684:692	arg1	scaffolds					694:702	collagen/chitosan (COL-CS) composite scaffolds	657:702	collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW	657:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	6	77	theme	cell	1013:1016	arg1	migration					1018:1026	cell migration	1013:1026	cell migration	1013:1026	In vitro studies revealed that the HBD-2 COL-CS scaffold was biocompatible and accelerated cell migration and angiogenesis.					
36934557	0	78	theme	diabetic	122:129	arg1	wounds					131:136	diabetic wounds	122:136	diabetic wounds	122:136	Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds.					
36934557	8	79	dep	in	1160:1161	arg1	vivo					1163:1166	vivo	1163:1166	vivo	1163:1166	The in vivo studies revealed that the HBD-2 COL-CS treated group accelerated healing compared to those in COL-CS and control groups.					
36934557	10	80	theme	anti-inflammatory	1608:1624	arg1	effect					1662:1667	positive angiogenic effect	1642:1667	positive angiogenic effect	1642:1667	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	5	81	theme	low	818:820	arg1	degradation					829:839	low matrix degradation	818:839	low matrix degradation	818:839	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	4	82	theme	beta	560:563	arg1	nanoparticle					629:640	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle	554:640	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW	554:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	10	83	theme	HBD-2	1601:1605	arg1	effects					1517:1523	the synergistic effects	1501:1523	the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release)	1501:1798	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	84	theme	treated	1471:1477	arg1	group					1479:1483	HBD-2 COL-CS treated group	1458:1483	HBD-2 COL-CS treated group	1458:1483	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	85	theme	positive	1642:1649	arg1	effect					1662:1667	positive angiogenic effect	1642:1667	positive angiogenic effect	1642:1667	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	5	86	theme	biodegradable	773:785	arg1	scaffold					799:806	the developed biodegradable crosslinked scaffold	759:806	the developed biodegradable crosslinked scaffold	759:806	Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold.					
36934557	3	87	theme	treatment	428:436	arg1	strategy					438:445	a single treatment strategy	419:445	a single treatment strategy to handle all the loopholes	419:473	Henceforth, developing a single treatment strategy to handle all the loopholes may effectively manage DW.					
36934557	10	88	theme	HBD-2	1458:1462	arg1	group					1479:1483	HBD-2 COL-CS treated group	1458:1483	HBD-2 COL-CS treated group	1458:1483	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	89	theme	antibacterial	1627:1639	arg1	effect					1662:1667	positive angiogenic effect	1642:1667	positive angiogenic effect	1642:1667	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	90	theme	cell	1670:1673	arg1	proliferation					1675:1687	cell proliferation	1670:1687	cell proliferation	1670:1687	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	4	91	theme	lactic-co-glycolic	597:614	arg1	Poly					591:594	loaded Poly	584:594	Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW	554:736	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	4	91	theme	lactic-co-glycolic	597:614	arg1	acid					616:619	lactic-co-glycolic acid	597:619	lactic-co-glycolic acid	597:619	The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW.					
36934557	10	92	dep	HBD-2	1601:1605	arg1	effect					1662:1667	positive angiogenic effect	1642:1667	positive angiogenic effect	1642:1667	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	92	dep	HBD-2	1601:1605	arg1	proliferation					1675:1687	cell proliferation	1670:1687	cell proliferation	1670:1687	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	10	92	dep	HBD-2	1601:1605	arg1	migration					1694:1702	migration	1694:1702	migration	1694:1702	The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).					
36934557	0	93	theme	beta	6:9	arg1	defensin-2					11:20	Human beta defensin-2	0:20	Human beta defensin-2	0:20	Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds.					
36934557	1	94	theme	diabetic	248:255	arg1	patients					257:264	diabetic patients	248:264	diabetic patients	248:264	Diabetic wound (DW) is the most devastating complication resulting in significant mortality and morbidity in diabetic patients.					
36203120	10	0	theme	high	1894:1897	arg1	operation					1810:1818	simple operation	1803:1818	simple operation	1803:1818	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	0	theme	high	1894:1897	arg1	recoveries					1826:1835	high recoveries	1821:1835	high recoveries	1821:1835	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	0	theme	high	1894:1897	arg1	consumption					1881:1891	desorption solvent consumption	1862:1891	desorption solvent consumption	1862:1891	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	0	theme	high	1894:1897	arg1	solution					1849:1856	low sample solution	1838:1856	low sample solution	1838:1856	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	0	theme	high	1894:1897	arg1	factors					1916:1922	high preconcentration factors	1894:1922	high preconcentration factors	1894:1922	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	6	1	theme	tetracycline	1280:1291	arg1	determination					1219:1231	the determination	1215:1231	the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively	1215:1305	The method has wide linearity in the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively.					
36203120	3	2	theme	Fe3O4	754:758	arg1	nanoparticles					760:772	Fe3O4 nanoparticles	754:772	Fe3O4 nanoparticles with chitosan	754:786	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	8	3	from	concentration	1516:1528	arg1	determination					1481:1493	the determination	1477:1493	the determination of the analytes at a concentration of 20.0 µg L-1	1477:1543	The relative standard deviations (RSDs) were also lower than 4.90% for the determination of the analytes at a concentration of 20.0 µg L-1.					
36203120	8	3	from	concentration	1516:1528	arg1	analytes					1502:1509	the analytes	1498:1509	the analytes at a concentration of 20.0 µg L-1	1498:1543	The relative standard deviations (RSDs) were also lower than 4.90% for the determination of the analytes at a concentration of 20.0 µg L-1.					
36203120	10	4	theme	DMSPE-HPLC-DAD	1710:1723	arg1	procedure					1725:1733	The DMSPE-HPLC-DAD procedure	1706:1733	The DMSPE-HPLC-DAD procedure	1706:1733	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	0	5	theme	Simultaneous	0:11	arg1	determination					13:25	Simultaneous determination	0:25	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.	0:190	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	10	6	theme	simple	1956:1961	arg1	method					1979:1984	simple and inexpensive method	1956:1984	simple and inexpensive method of sorbent preparation	1956:2007	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	2	7	theme	antibiotics	595:605	arg1	extraction					577:586	the simultaneous extraction	560:586	the simultaneous extraction of the antibiotics in biological samples	560:627	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	6	8	theme	2.0-800.0	1141:1149	arg1	ranges					1108:1113	the concentration ranges	1090:1113	the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively	1090:1305	The method has wide linearity in the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively.					
36203120	9	9	with	recoveries	1551:1560	arg1	RSDs					1609:1612	RSDs	1609:1612	RSDs between 4.64 and 5.83%	1609:1635	High recoveries in the percentage range of 94.70-106.75%, with RSDs between 4.64 and 5.83%, were obtained to analyze spiked urine and hair samples.					
36203120	0	10	theme	dispersive	112:121	arg1	chromatography					176:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	9	11	theme	spiked	1663:1668	arg1	samples					1685:1691	spiked urine and hair samples	1663:1691	spiked urine and hair samples	1663:1691	High recoveries in the percentage range of 94.70-106.75%, with RSDs between 4.64 and 5.83%, were obtained to analyze spiked urine and hair samples.					
36203120	6	12	theme	azithromycin	1236:1247	arg1	determination					1219:1231	the determination	1215:1231	the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively	1215:1305	The method has wide linearity in the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively.					
36203120	0	13	theme	solid-phase	129:139	arg1	chromatography					176:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	1	14	theme	side	435:438	arg1	effects					440:446	their side effects	429:446	their side effects	429:446	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	10	15	theme	inexpensive	1967:1977	arg1	method					1979:1984	simple and inexpensive method	1956:1984	simple and inexpensive method of sorbent preparation	1956:2007	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	0	16	theme	performance	157:167	arg1	chromatography					176:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	2	17	theme	biological	610:619	arg1	samples					621:627	biological samples	610:627	biological samples	610:627	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	10	18	theme	high	1821:1824	arg1	recoveries					1826:1835	high recoveries	1821:1835	high recoveries	1821:1835	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	19	theme	preparation	1997:2007	arg1	recoveries					1826:1835	high recoveries	1821:1835	high recoveries	1821:1835	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	19	theme	preparation	1997:2007	arg1	consumption					1881:1891	desorption solvent consumption	1862:1891	desorption solvent consumption	1862:1891	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	19	theme	preparation	1997:2007	arg1	method					1979:1984	simple and inexpensive method	1956:1984	simple and inexpensive method of sorbent preparation	1956:2007	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	19	theme	preparation	1997:2007	arg1	operation					1810:1818	simple operation	1803:1818	simple operation	1803:1818	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	19	theme	preparation	1997:2007	arg1	time					1946:1949	proper determination time	1925:1949	proper determination time	1925:1949	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	19	theme	preparation	1997:2007	arg1	solution					1849:1856	low sample solution	1838:1856	low sample solution	1838:1856	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	19	theme	preparation	1997:2007	arg1	antibiotics					1780:1790	antibiotics	1780:1790	antibiotics	1780:1790	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	19	theme	preparation	1997:2007	arg1	factors					1916:1922	high preconcentration factors	1894:1922	high preconcentration factors	1894:1922	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	3	20	theme	@	656:656	arg1	sorbent					715:721	a green and magnetic sorbent	694:721	sorbent	715:721	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	3	20	theme	@	656:656	arg1	nanoparticles					663:675	chitosan@Fe3O4 nanoparticles	648:675	chitosan@Fe3O4 nanoparticles	648:675	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	2	21	theme	sample	523:528	arg1	preparation					530:540	an efficient sample preparation	510:540	an efficient sample preparation	510:540	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	6	22	contain	has	1068:1070	arg2	linearity					1077:1085	wide linearity	1072:1085	wide linearity	1072:1085	The method has wide linearity in the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively.					
36203120	6	22	contain	has	1068:1070	arg1	method					1061:1066	The method	1057:1066	The method	1057:1066	The method has wide linearity in the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively.					
36203120	10	23	theme	determination	1932:1944	arg1	time					1946:1949	proper determination time	1925:1949	proper determination time	1925:1949	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	2	24	theme	simultaneous	564:575	arg1	extraction					577:586	the simultaneous extraction	560:586	the simultaneous extraction of the antibiotics in biological samples	560:627	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	6	25	theme	wide	1072:1075	arg1	linearity					1077:1085	wide linearity	1072:1085	wide linearity	1072:1085	The method has wide linearity in the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively.					
36203120	10	26	theme	proper	1925:1930	arg1	time					1946:1949	proper determination time	1925:1949	proper determination time	1925:1949	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	8	27	from	determination	1481:1493	arg1	concentration					1516:1528	a concentration	1514:1528	a concentration of 20.0 µg L-1	1514:1543	The relative standard deviations (RSDs) were also lower than 4.90% for the determination of the analytes at a concentration of 20.0 µg L-1.					
36203120	5	28	theme	antibiotic	947:956	arg1	extraction					958:967	antibiotic extraction	947:967	antibiotic extraction	947:967	In addition, effective factors for antibiotic extraction were evaluated and optimized using a central composite design and one factor at a time.					
36203120	7	29	theme	detection	1321:1329	arg1	limit					1312:1316	The limit	1308:1316	The limit of detection and limit of quantitation	1308:1355	The limit of detection and limit of quantitation was lower than 1.1 and 3.5 μg L-1, respectively.					
36203120	7	29	theme	detection	1321:1329	arg1	lower					1361:1365	lower	1361:1365	lower	1361:1365	The limit of detection and limit of quantitation was lower than 1.1 and 3.5 μg L-1, respectively.					
36203120	2	30	theme	solid-phase	476:486	arg1	extraction					488:497	A dispersive micro solid-phase extraction	457:497	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation	449:540	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	2	30	theme	solid-phase	476:486	arg1	DMSPE					500:504	DMSPE	500:504	DMSPE	500:504	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	2	31	dep	METHODS	449:455	arg1	extraction					488:497	A dispersive micro solid-phase extraction	457:497	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation	449:540	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	2	31	dep	METHODS	449:455	arg1	DMSPE					500:504	DMSPE	500:504	DMSPE	500:504	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	2	32	theme	dispersive	459:468	arg1	extraction					488:497	A dispersive micro solid-phase extraction	457:497	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation	449:540	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	2	32	theme	dispersive	459:468	arg1	DMSPE					500:504	DMSPE	500:504	DMSPE	500:504	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	1	33	used	utilized	308:315	arg2	Antibiotics					203:213	BACKGROUND Antibiotics	192:213	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin	192:295	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	8	34	theme	20.0 µg L-1	1533:1543	arg1	concentration					1516:1528	a concentration	1514:1528	a concentration of 20.0 µg L-1	1514:1543	The relative standard deviations (RSDs) were also lower than 4.90% for the determination of the analytes at a concentration of 20.0 µg L-1.					
36203120	1	35	contain	containing	215:224	arg2	doxycycline					253:263	doxycycline	253:263	doxycycline	253:263	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	1	35	contain	containing	215:224	arg2	azithromycin					284:295	azithromycin	284:295	azithromycin	284:295	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	1	35	contain	containing	215:224	arg2	azithromycin					226:237	azithromycin	226:237	azithromycin	226:237	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	1	35	contain	containing	215:224	arg2	tetracycline					266:277	tetracycline	266:277	tetracycline	266:277	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	1	35	contain	containing	215:224	arg2	amoxicillin					240:250	amoxicillin	240:250	amoxicillin	240:250	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	1	35	contain	containing	215:224	arg1	Antibiotics					203:213	BACKGROUND Antibiotics	192:213	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin	192:295	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	4	36	theme	ZnO	852:854	arg1	NPs					856:858	ZnO NPs	852:858	ZnO NPs	852:858	RESULTS The sorbent was compared with several sorbents such as ZnO NPs, CuO NPs, and Fe3O4 NPs to extract the antibiotics.					
36203120	0	37	theme	antibiotics	35:45	arg1	determination					13:25	Simultaneous determination	0:25	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.	0:190	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	1	38	theme	bacterial	337:345	arg1	infections					347:356	bacterial infections	337:356	bacterial infections	337:356	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	10	39	theme	low	1838:1840	arg1	solution					1849:1856	low sample solution	1838:1856	low sample solution	1838:1856	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	0	40	theme	human	50:54	arg1	samples					71:77	human urine and hair samples	50:77	human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	50:189	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	10	41	theme	simple	1803:1808	arg1	operation					1810:1818	simple operation	1803:1818	simple operation	1803:1818	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	42	theme	desorption	1862:1871	arg1	consumption					1881:1891	desorption solvent consumption	1862:1891	desorption solvent consumption	1862:1891	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	8	43	theme	standard	1419:1426	arg1	RSDs					1440:1443	RSDs	1440:1443	RSDs	1440:1443	The relative standard deviations (RSDs) were also lower than 4.90% for the determination of the analytes at a concentration of 20.0 µg L-1.					
36203120	8	43	theme	standard	1419:1426	arg1	deviations					1428:1437	The relative standard deviations	1406:1437	The relative standard deviations (RSDs)	1406:1444	The relative standard deviations (RSDs) were also lower than 4.90% for the determination of the analytes at a concentration of 20.0 µg L-1.					
36203120	8	43	theme	standard	1419:1426	arg1	lower					1456:1460	lower	1456:1460	lower	1456:1460	The relative standard deviations (RSDs) were also lower than 4.90% for the determination of the analytes at a concentration of 20.0 µg L-1.					
36203120	5	44	theme	central	1006:1012	arg1	design					1024:1029	a central composite design	1004:1029	a central composite design	1004:1029	In addition, effective factors for antibiotic extraction were evaluated and optimized using a central composite design and one factor at a time.					
36203120	2	45	from	extraction	577:586	arg1	samples					621:627	biological samples	610:627	biological samples	610:627	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	3	46	with	nanoparticles	760:772	arg1	chitosan					779:786	chitosan	779:786	chitosan	779:786	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	6	47	theme	concentration	1094:1106	arg1	ranges					1108:1113	the concentration ranges	1090:1113	the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively	1090:1305	The method has wide linearity in the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively.					
36203120	3	48	theme	chemical	734:741	arg1	coating					743:749	a chemical coating	732:749	a chemical coating of Fe3O4 nanoparticles with chitosan	732:786	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	4	49	theme	Fe3O4	874:878	arg1	NPs					880:882	Fe3O4 NPs	874:882	Fe3O4 NPs	874:882	RESULTS The sorbent was compared with several sorbents such as ZnO NPs, CuO NPs, and Fe3O4 NPs to extract the antibiotics.					
36203120	2	50	from	samples	621:627	arg1	extraction					577:586	the simultaneous extraction	560:586	the simultaneous extraction of the antibiotics in biological samples	560:627	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	3	51	theme	nanoparticles	760:772	arg1	coating					743:749	a chemical coating	732:749	a chemical coating of Fe3O4 nanoparticles with chitosan	732:786	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	6	52	theme	doxycycline	1263:1273	arg1	determination					1219:1231	the determination	1215:1231	the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively	1215:1305	The method has wide linearity in the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively.					
36203120	1	53	theme	BACKGROUND	192:201	arg1	Antibiotics					203:213	BACKGROUND Antibiotics	192:213	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin	192:295	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	1	54	from	determination	369:381	arg1	samples					397:403	biological samples	386:403	biological samples	386:403	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	4	55	theme	CuO	861:863	arg1	NPs					865:867	CuO NPs	861:867	CuO NPs	861:867	RESULTS The sorbent was compared with several sorbents such as ZnO NPs, CuO NPs, and Fe3O4 NPs to extract the antibiotics.					
36203120	6	56	theme	2.7-1000.0 μg L-1	1156:1172	arg1	ranges					1108:1113	the concentration ranges	1090:1113	the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively	1090:1305	The method has wide linearity in the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively.					
36203120	6	57	theme	amoxicillin	1250:1260	arg1	determination					1219:1231	the determination	1215:1231	the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively	1215:1305	The method has wide linearity in the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively.					
36203120	3	58	theme	magnetic	706:713	arg1	nanoparticles					663:675	chitosan@Fe3O4 nanoparticles	648:675	chitosan@Fe3O4 nanoparticles	648:675	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	3	58	theme	magnetic	706:713	arg1	sorbent					715:721	a green and magnetic sorbent	694:721	sorbent	715:721	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	0	59	theme	micro	123:127	arg1	chromatography					176:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	10	60	theme	various	1745:1751	arg1	advantages					1753:1762	various advantages	1745:1762	various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation	1745:2007	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	0	61	theme	extraction-high	141:155	arg1	chromatography					176:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	9	62	theme	urine	1670:1674	arg1	samples					1685:1691	spiked urine and hair samples	1663:1691	spiked urine and hair samples	1663:1691	High recoveries in the percentage range of 94.70-106.75%, with RSDs between 4.64 and 5.83%, were obtained to analyze spiked urine and hair samples.					
36203120	0	63	theme	liquid	169:174	arg1	chromatography					176:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	4	64	dep	RESULTS	789:795	arg1	compared					813:820	compared	813:820	was compared with several sorbents such as ZnO NPs, CuO NPs, and Fe3O4 NPs to extract the antibiotics	809:909	RESULTS The sorbent was compared with several sorbents such as ZnO NPs, CuO NPs, and Fe3O4 NPs to extract the antibiotics.					
36203120	9	65	theme	hair	1680:1683	arg1	samples					1685:1691	spiked urine and hair samples	1663:1691	spiked urine and hair samples	1663:1691	High recoveries in the percentage range of 94.70-106.75%, with RSDs between 4.64 and 5.83%, were obtained to analyze spiked urine and hair samples.					
36203120	4	66	theme	several	827:833	arg1	sorbents					835:842	several sorbents	827:842	several sorbents such as ZnO NPs, CuO NPs, and Fe3O4 NPs	827:882	RESULTS The sorbent was compared with several sorbents such as ZnO NPs, CuO NPs, and Fe3O4 NPs to extract the antibiotics.					
36203120	4	66	theme	several	827:833	arg1	NPs					880:882	Fe3O4 NPs	874:882	Fe3O4 NPs	874:882	RESULTS The sorbent was compared with several sorbents such as ZnO NPs, CuO NPs, and Fe3O4 NPs to extract the antibiotics.					
36203120	4	66	theme	several	827:833	arg1	NPs					856:858	ZnO NPs	852:858	ZnO NPs	852:858	RESULTS The sorbent was compared with several sorbents such as ZnO NPs, CuO NPs, and Fe3O4 NPs to extract the antibiotics.					
36203120	4	66	theme	several	827:833	arg1	NPs					865:867	CuO NPs	861:867	CuO NPs	861:867	RESULTS The sorbent was compared with several sorbents such as ZnO NPs, CuO NPs, and Fe3O4 NPs to extract the antibiotics.					
36203120	3	67	theme	Fe3O4	657:661	arg1	sorbent					715:721	a green and magnetic sorbent	694:721	sorbent	715:721	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	3	67	theme	Fe3O4	657:661	arg1	nanoparticles					663:675	chitosan@Fe3O4 nanoparticles	648:675	chitosan@Fe3O4 nanoparticles	648:675	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	5	68	theme	effective	925:933	arg1	factors					935:941	effective factors	925:941	effective factors for antibiotic extraction	925:967	In addition, effective factors for antibiotic extraction were evaluated and optimized using a central composite design and one factor at a time.					
36203120	0	69	theme	hair	66:69	arg1	samples					71:77	human urine and hair samples	50:77	human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	50:189	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	3	70	theme	chitosan	648:655	arg1	sorbent					715:721	a green and magnetic sorbent	694:721	sorbent	715:721	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	3	70	theme	chitosan	648:655	arg1	nanoparticles					663:675	chitosan@Fe3O4 nanoparticles	648:675	chitosan@Fe3O4 nanoparticles	648:675	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	9	71	from	recoveries	1551:1560	arg1	range					1580:1584	the percentage range	1565:1584	the percentage range of 94.70-106.75%	1565:1601	High recoveries in the percentage range of 94.70-106.75%, with RSDs between 4.64 and 5.83%, were obtained to analyze spiked urine and hair samples.					
36203120	7	72	theme	quantitation	1344:1355	arg1	detection					1321:1329	detection	1321:1329	detection	1321:1329	The limit of detection and limit of quantitation was lower than 1.1 and 3.5 μg L-1, respectively.					
36203120	7	72	theme	quantitation	1344:1355	arg1	limit					1335:1339	limit	1335:1339	limit	1335:1339	The limit of detection and limit of quantitation was lower than 1.1 and 3.5 μg L-1, respectively.					
36203120	10	73	dep	CONCLUSIONS	1694:1704	arg1	displayed					1735:1743	displayed	1735:1743	displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation	1735:2007	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	74	theme	sorbent	1989:1995	arg1	preparation					1997:2007	sorbent preparation	1989:2007	sorbent preparation	1989:2007	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	2	75	theme	efficient	513:521	arg1	preparation					530:540	an efficient sample preparation	510:540	an efficient sample preparation	510:540	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	0	76	from	determination	13:25	arg1	samples					71:77	human urine and hair samples	50:77	human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	50:189	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	0	77	theme	simple	91:96	arg1	chromatography					176:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	1	78	theme	biological	386:395	arg1	samples					397:403	biological samples	386:403	biological samples	386:403	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	8	79	theme	analytes	1502:1509	arg1	determination					1481:1493	the determination	1477:1493	the determination of the analytes at a concentration of 20.0 µg L-1	1477:1543	The relative standard deviations (RSDs) were also lower than 4.90% for the determination of the analytes at a concentration of 20.0 µg L-1.					
36203120	0	80	theme	efficient	102:110	arg1	chromatography					176:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	89:189	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	9	81	theme	High	1546:1549	arg1	recoveries					1551:1560	High recoveries	1546:1560	High recoveries	1546:1560	High recoveries in the percentage range of 94.70-106.75%, with RSDs between 4.64 and 5.83%, were obtained to analyze spiked urine and hair samples.					
36203120	2	82	theme	micro	470:474	arg1	extraction					488:497	A dispersive micro solid-phase extraction	457:497	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation	449:540	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	2	82	theme	micro	470:474	arg1	DMSPE					500:504	DMSPE	500:504	DMSPE	500:504	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	10	83	theme	sample	1842:1847	arg1	solution					1849:1856	low sample solution	1838:1856	low sample solution	1838:1856	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	9	84	theme	percentage	1569:1578	arg1	range					1580:1584	the percentage range	1565:1584	the percentage range of 94.70-106.75%	1565:1601	High recoveries in the percentage range of 94.70-106.75%, with RSDs between 4.64 and 5.83%, were obtained to analyze spiked urine and hair samples.					
36203120	0	85	theme	urine	56:60	arg1	samples					71:77	human urine and hair samples	50:77	human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography	50:189	Simultaneous determination of four antibiotics in human urine and hair samples employing a simple and efficient dispersive micro solid-phase extraction-high performance liquid chromatography.					
36203120	1	86	theme	infections	347:356	arg1	treatment					324:332	the treatment	320:332	the treatment of bacterial infections	320:356	BACKGROUND Antibiotics containing azithromycin, amoxicillin, doxycycline, tetracycline, and azithromycin are widely utilized in the treatment of bacterial infections, and their determination in biological samples is critical to evaluate their side effects.					
36203120	6	87	theme	3.5-1000.0	1129:1138	arg1	ranges					1108:1113	the concentration ranges	1090:1113	the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively	1090:1305	The method has wide linearity in the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively.					
36203120	10	88	theme	solvent	1873:1879	arg1	consumption					1881:1891	desorption solvent consumption	1862:1891	desorption solvent consumption	1862:1891	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	9	89	theme	%	1601:1601	arg1	range					1580:1584	the percentage range	1565:1584	the percentage range of 94.70-106.75%	1565:1601	High recoveries in the percentage range of 94.70-106.75%, with RSDs between 4.64 and 5.83%, were obtained to analyze spiked urine and hair samples.					
36203120	6	90	theme	2.1-800.0	1118:1126	arg1	ranges					1108:1113	the concentration ranges	1090:1113	the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively	1090:1305	The method has wide linearity in the concentration ranges of 2.1-800.0, 3.5-1000.0, 2.0-800.0, and 2.7-1000.0 μg L-1 with an R-squared higher than 0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and tetracycline, respectively.					
36203120	3	91	theme	green	696:700	arg1	nanoparticles					663:675	chitosan@Fe3O4 nanoparticles	648:675	chitosan@Fe3O4 nanoparticles	648:675	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	3	91	theme	green	696:700	arg1	sorbent					715:721	a green and magnetic sorbent	694:721	sorbent	715:721	For this purpose, chitosan@Fe3O4 nanoparticles were prepared as a green and magnetic sorbent based on a chemical coating of Fe3O4 nanoparticles with chitosan.					
36203120	8	92	theme	relative	1410:1417	arg1	RSDs					1440:1443	RSDs	1440:1443	RSDs	1440:1443	The relative standard deviations (RSDs) were also lower than 4.90% for the determination of the analytes at a concentration of 20.0 µg L-1.					
36203120	8	92	theme	relative	1410:1417	arg1	deviations					1428:1437	The relative standard deviations	1406:1437	The relative standard deviations (RSDs)	1406:1444	The relative standard deviations (RSDs) were also lower than 4.90% for the determination of the analytes at a concentration of 20.0 µg L-1.					
36203120	8	92	theme	relative	1410:1417	arg1	lower					1456:1460	lower	1456:1460	lower	1456:1460	The relative standard deviations (RSDs) were also lower than 4.90% for the determination of the analytes at a concentration of 20.0 µg L-1.					
36203120	5	93	theme	composite	1014:1022	arg1	design					1024:1029	a central composite design	1004:1029	a central composite design	1004:1029	In addition, effective factors for antibiotic extraction were evaluated and optimized using a central composite design and one factor at a time.					
36203120	7	94	theme	limit	1335:1339	arg1	limit					1312:1316	The limit	1308:1316	The limit of detection and limit of quantitation	1308:1355	The limit of detection and limit of quantitation was lower than 1.1 and 3.5 μg L-1, respectively.					
36203120	7	94	theme	limit	1335:1339	arg1	lower					1361:1365	lower	1361:1365	lower	1361:1365	The limit of detection and limit of quantitation was lower than 1.1 and 3.5 μg L-1, respectively.					
36203120	2	95	from	antibiotics	595:605	arg1	samples					621:627	biological samples	610:627	biological samples	610:627	METHODS A dispersive micro solid-phase extraction (DMSPE) as an efficient sample preparation was developed for the simultaneous extraction of the antibiotics in biological samples.					
36203120	10	96	theme	preconcentration	1899:1914	arg1	operation					1810:1818	simple operation	1803:1818	simple operation	1803:1818	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	96	theme	preconcentration	1899:1914	arg1	recoveries					1826:1835	high recoveries	1821:1835	high recoveries	1821:1835	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	96	theme	preconcentration	1899:1914	arg1	consumption					1881:1891	desorption solvent consumption	1862:1891	desorption solvent consumption	1862:1891	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	96	theme	preconcentration	1899:1914	arg1	solution					1849:1856	low sample solution	1838:1856	low sample solution	1838:1856	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36203120	10	96	theme	preconcentration	1899:1914	arg1	factors					1916:1922	high preconcentration factors	1894:1922	high preconcentration factors	1894:1922	CONCLUSIONS The DMSPE-HPLC-DAD procedure displayed various advantages for determining antibiotics, including simple operation, high recoveries, low sample solution and desorption solvent consumption, high preconcentration factors, proper determination time, and simple and inexpensive method of sorbent preparation.					
36693509	7	0	theme	by-products	1035:1045	arg1	pretreatment					1003:1014	the pretreatment	999:1014	the pretreatment of agro-industrial by-products	999:1045	Imidazole is then emerging as a potential catalyst for the pretreatment of agro-industrial by-products, allowing the valorisation of these residues and their reinsertion into the production chain under a biorefinery concept.					
36693509	6	1	theme	enzymatic	848:856	arg1	conversion					858:867	an enzymatic conversion	845:867	an enzymatic conversion greater than 95%	845:884	Levels of up to 72% delignification were obtained, which allowed an enzymatic conversion greater than 95% for SB, SH and OPEFB, while only 83% was reached for CPH.					
36693509	3	2	theme	cocoa	304:308	arg1	CPH					321:323	CPH	321:323	CPH	321:323	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	3	2	theme	cocoa	304:308	arg1	husks					314:318	cocoa pod husks	304:318	cocoa pod husks (CPH)	304:324	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	1	3	theme	great	159:163	arg1	abundance					165:173	great abundance	159:173	great abundance	159:173	Lignocellulosic biomass is a renewable material of great abundance.					
36693509	6	4	dep	conversion	858:867	arg1	%					884:884	%	884:884	%	884:884	Levels of up to 72% delignification were obtained, which allowed an enzymatic conversion greater than 95% for SB, SH and OPEFB, while only 83% was reached for CPH.					
36693509	0	5	theme	lignocellulosic	83:97	arg1	biomass					99:105	agro-industrial lignocellulosic biomass	67:105	agro-industrial lignocellulosic biomass	67:105	The potential of imidazole as a new solvent in the pretreatment of agro-industrial lignocellulosic biomass.					
36693509	0	6	theme	agro-industrial	67:81	arg1	biomass					99:105	agro-industrial lignocellulosic biomass	67:105	agro-industrial lignocellulosic biomass	67:105	The potential of imidazole as a new solvent in the pretreatment of agro-industrial lignocellulosic biomass.					
36693509	0	7	from	solvent	36:42	arg1	pretreatment					51:62	the pretreatment	47:62	the pretreatment of agro-industrial lignocellulosic biomass	47:105	The potential of imidazole as a new solvent in the pretreatment of agro-industrial lignocellulosic biomass.					
36693509	7	8	theme	agro-industrial	1019:1033	arg1	by-products					1035:1045	agro-industrial by-products	1019:1045	agro-industrial by-products	1019:1045	Imidazole is then emerging as a potential catalyst for the pretreatment of agro-industrial by-products, allowing the valorisation of these residues and their reinsertion into the production chain under a biorefinery concept.					
36693509	4	9	theme	higher	547:552	arg1	content					554:560	higher content	547:560	higher content of holocellulose	547:577	Non-treated SH, SB and OPEFB have higher content of holocellulose, while CPH is rich in lignin polymers (31.2%).					
36693509	3	10	theme	composition	438:448	arg1	variations					450:459	chemical composition variations	429:459	chemical composition variations	429:459	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	5	11	theme	cellulose	769:777	arg1	percentage					755:764	the percentage	751:764	the percentage of cellulose	751:777	After imidazole-pretreatment, all biomasses presented structural disorganization of lignocellulosic fibres and enrichment in the percentage of cellulose.					
36693509	3	12	theme	hydrolysis	490:499	arg1	efficiency					501:510	enzymatic hydrolysis efficiency	480:510	enzymatic hydrolysis efficiency	480:510	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	1	13	theme	abundance	165:173	arg1	material					147:154	a renewable material	135:154	a renewable material of great abundance	135:173	Lignocellulosic biomass is a renewable material of great abundance.					
36693509	1	13	theme	abundance	165:173	arg1	biomass					124:130	Lignocellulosic biomass	108:130	Lignocellulosic biomass	108:130	Lignocellulosic biomass is a renewable material of great abundance.					
36693509	0	14	theme	biomass	99:105	arg1	pretreatment					51:62	the pretreatment	47:62	the pretreatment of agro-industrial lignocellulosic biomass	47:105	The potential of imidazole as a new solvent in the pretreatment of agro-industrial lignocellulosic biomass.					
36693509	7	15	theme	potential	976:984	arg1	catalyst					986:993	a potential catalyst	974:993	a potential catalyst for the pretreatment of agro-industrial by-products	974:1045	Imidazole is then emerging as a potential catalyst for the pretreatment of agro-industrial by-products, allowing the valorisation of these residues and their reinsertion into the production chain under a biorefinery concept.					
36693509	4	16	from	polymers	608:615	arg1	rich					593:596	rich	593:596	rich	593:596	Non-treated SH, SB and OPEFB have higher content of holocellulose, while CPH is rich in lignin polymers (31.2%).					
36693509	3	17	theme	imidazole	385:393	arg1	pretreatment					395:406	imidazole pretreatment	385:406	imidazole pretreatment	385:406	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	3	18	theme	empty	339:343	arg1	OPEFB					360:364	OPEFB	360:364	OPEFB	360:364	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	3	18	theme	empty	339:343	arg1	bunches					351:357	oil palm empty fruit bunches	330:357	oil palm empty fruit bunches (OPEFB)	330:365	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	5	19	theme	enrichment	737:746	arg1	disorganization					691:705	structural disorganization	680:705	structural disorganization of lignocellulosic fibres and enrichment	680:746	After imidazole-pretreatment, all biomasses presented structural disorganization of lignocellulosic fibres and enrichment in the percentage of cellulose.					
36693509	5	20	theme	lignocellulosic	710:724	arg1	fibres					726:731	lignocellulosic fibres	710:731	lignocellulosic fibres	710:731	After imidazole-pretreatment, all biomasses presented structural disorganization of lignocellulosic fibres and enrichment in the percentage of cellulose.					
36693509	3	21	theme	fruit	345:349	arg1	OPEFB					360:364	OPEFB	360:364	OPEFB	360:364	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	3	21	theme	fruit	345:349	arg1	bunches					351:357	oil palm empty fruit bunches	330:357	oil palm empty fruit bunches (OPEFB)	330:365	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	3	22	theme	Sugarcane	260:268	arg1	SB					279:280	SB	279:280	SB	279:280	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	3	22	theme	Sugarcane	260:268	arg1	bagasse					270:276	Sugarcane bagasse	260:276	Sugarcane bagasse (SB)	260:281	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	7	23	theme	biorefinery	1148:1158	arg1	concept					1160:1166	a biorefinery concept	1146:1166	a biorefinery concept	1146:1166	Imidazole is then emerging as a potential catalyst for the pretreatment of agro-industrial by-products, allowing the valorisation of these residues and their reinsertion into the production chain under a biorefinery concept.					
36693509	3	24	theme	oil	330:332	arg1	OPEFB					360:364	OPEFB	360:364	OPEFB	360:364	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	3	24	theme	oil	330:332	arg1	bunches					351:357	oil palm empty fruit bunches	330:357	oil palm empty fruit bunches (OPEFB)	330:365	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	4	25	from	rich	593:596	arg1	polymers					608:615	lignin polymers	601:615	lignin polymers (31.2%)	601:623	Non-treated SH, SB and OPEFB have higher content of holocellulose, while CPH is rich in lignin polymers (31.2%).					
36693509	4	25	from	rich	593:596	arg1	%					622:622	31.2%	618:622	31.2%	618:622	Non-treated SH, SB and OPEFB have higher content of holocellulose, while CPH is rich in lignin polymers (31.2%).					
36693509	3	26	theme	palm	334:337	arg1	OPEFB					360:364	OPEFB	360:364	OPEFB	360:364	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	3	26	theme	palm	334:337	arg1	bunches					351:357	oil palm empty fruit bunches	330:357	oil palm empty fruit bunches (OPEFB)	330:365	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	6	27	dep	%	798:798	arg1	72					796:797	72	796:797	72	796:797	Levels of up to 72% delignification were obtained, which allowed an enzymatic conversion greater than 95% for SB, SH and OPEFB, while only 83% was reached for CPH.					
36693509	3	28	theme	soybean	284:290	arg1	SH					299:300	SH	299:300	SH	299:300	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	3	28	theme	soybean	284:290	arg1	hulls					292:296	soybean hulls	284:296	soybean hulls (SH)	284:301	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	6	29	dep	72	796:797	arg1	to					793:794	to	793:794	to	793:794	Levels of up to 72% delignification were obtained, which allowed an enzymatic conversion greater than 95% for SB, SH and OPEFB, while only 83% was reached for CPH.					
36693509	6	30	dep	%	884:884	arg1	95					882:883	95	882:883	95	882:883	Levels of up to 72% delignification were obtained, which allowed an enzymatic conversion greater than 95% for SB, SH and OPEFB, while only 83% was reached for CPH.					
36693509	2	31	theme	pretreatments	245:257	arg1	application					230:240	the application	226:240	the application of pretreatments	226:257	However, its recalcitrant characteristic requires the application of pretreatments.					
36693509	0	32	theme	imidazole	17:25	arg1	potential					4:12	The potential	0:12	The potential of imidazole as a new solvent in the pretreatment of agro-industrial lignocellulosic biomass.	0:106	The potential of imidazole as a new solvent in the pretreatment of agro-industrial lignocellulosic biomass.					
36693509	6	33	dep	delignification	800:814	arg1	%					798:798	%	798:798	%	798:798	Levels of up to 72% delignification were obtained, which allowed an enzymatic conversion greater than 95% for SB, SH and OPEFB, while only 83% was reached for CPH.					
36693509	6	34	theme	delignification	800:814	arg1	Levels					780:785	Levels	780:785	Levels of up to 72% delignification	780:814	Levels of up to 72% delignification were obtained, which allowed an enzymatic conversion greater than 95% for SB, SH and OPEFB, while only 83% was reached for CPH.					
36693509	1	35	theme	Lignocellulosic	108:122	arg1	material					147:154	a renewable material	135:154	a renewable material of great abundance	135:173	Lignocellulosic biomass is a renewable material of great abundance.					
36693509	1	35	theme	Lignocellulosic	108:122	arg1	biomass					124:130	Lignocellulosic biomass	108:130	Lignocellulosic biomass	108:130	Lignocellulosic biomass is a renewable material of great abundance.					
36693509	0	36	theme	new	32:34	arg1	solvent					36:42	a new solvent	30:42	a new solvent in the pretreatment of agro-industrial lignocellulosic biomass	30:105	The potential of imidazole as a new solvent in the pretreatment of agro-industrial lignocellulosic biomass.					
36693509	4	37	theme	Non-treated	513:523	arg1	SH					525:526	Non-treated SH	513:526	Non-treated SH	513:526	Non-treated SH, SB and OPEFB have higher content of holocellulose, while CPH is rich in lignin polymers (31.2%).					
36693509	4	38	contain	have	542:545	arg1	SB					529:530	SB	529:530	SB	529:530	Non-treated SH, SB and OPEFB have higher content of holocellulose, while CPH is rich in lignin polymers (31.2%).					
36693509	4	38	contain	have	542:545	arg1	OPEFB					536:540	OPEFB	536:540	OPEFB	536:540	Non-treated SH, SB and OPEFB have higher content of holocellulose, while CPH is rich in lignin polymers (31.2%).					
36693509	4	38	contain	have	542:545	arg2	content					554:560	higher content	547:560	higher content of holocellulose	547:577	Non-treated SH, SB and OPEFB have higher content of holocellulose, while CPH is rich in lignin polymers (31.2%).					
36693509	4	38	contain	have	542:545	arg1	SH					525:526	Non-treated SH	513:526	Non-treated SH	513:526	Non-treated SH, SB and OPEFB have higher content of holocellulose, while CPH is rich in lignin polymers (31.2%).					
36693509	7	39	theme	production	1123:1132	arg1	chain					1134:1138	the production chain	1119:1138	the production chain	1119:1138	Imidazole is then emerging as a potential catalyst for the pretreatment of agro-industrial by-products, allowing the valorisation of these residues and their reinsertion into the production chain under a biorefinery concept.					
36693509	4	40	theme	holocellulose	565:577	arg1	content					554:560	higher content	547:560	higher content of holocellulose	547:577	Non-treated SH, SB and OPEFB have higher content of holocellulose, while CPH is rich in lignin polymers (31.2%).					
36693509	2	41	theme	recalcitrant	189:200	arg1	characteristic					202:215	its recalcitrant characteristic	185:215	its recalcitrant characteristic	185:215	However, its recalcitrant characteristic requires the application of pretreatments.					
36693509	3	42	theme	chemical	429:436	arg1	variations					450:459	chemical composition variations	429:459	chemical composition variations	429:459	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	3	43	theme	pod	310:312	arg1	CPH					321:323	CPH	321:323	CPH	321:323	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	3	43	theme	pod	310:312	arg1	husks					314:318	cocoa pod husks	304:318	cocoa pod husks (CPH)	304:324	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	5	44	attach	presented	670:678	arg2	biomasses					660:668	all biomasses	656:668	all biomasses	656:668	After imidazole-pretreatment, all biomasses presented structural disorganization of lignocellulosic fibres and enrichment in the percentage of cellulose.					
36693509	5	44	attach	presented	670:678	arg1	percentage					755:764	the percentage	751:764	the percentage of cellulose	751:777	After imidazole-pretreatment, all biomasses presented structural disorganization of lignocellulosic fibres and enrichment in the percentage of cellulose.					
36693509	1	45	theme	renewable	137:145	arg1	material					147:154	a renewable material	135:154	a renewable material of great abundance	135:173	Lignocellulosic biomass is a renewable material of great abundance.					
36693509	1	45	theme	renewable	137:145	arg1	biomass					124:130	Lignocellulosic biomass	108:130	Lignocellulosic biomass	108:130	Lignocellulosic biomass is a renewable material of great abundance.					
36693509	4	46	theme	lignin	601:606	arg1	polymers					608:615	lignin polymers	601:615	lignin polymers (31.2%)	601:623	Non-treated SH, SB and OPEFB have higher content of holocellulose, while CPH is rich in lignin polymers (31.2%).					
36693509	4	46	theme	lignin	601:606	arg1	%					622:622	31.2%	618:622	31.2%	618:622	Non-treated SH, SB and OPEFB have higher content of holocellulose, while CPH is rich in lignin polymers (31.2%).					
36693509	5	47	theme	fibres	726:731	arg1	disorganization					691:705	structural disorganization	680:705	structural disorganization of lignocellulosic fibres and enrichment	680:746	After imidazole-pretreatment, all biomasses presented structural disorganization of lignocellulosic fibres and enrichment in the percentage of cellulose.					
36693509	7	48	theme	residues	1083:1090	arg1	valorisation					1061:1072	the valorisation	1057:1072	the valorisation of these residues	1057:1090	Imidazole is then emerging as a potential catalyst for the pretreatment of agro-industrial by-products, allowing the valorisation of these residues and their reinsertion into the production chain under a biorefinery concept.					
36693509	7	48	theme	residues	1083:1090	arg1	reinsertion					1102:1112	their reinsertion	1096:1112	their reinsertion into the production chain	1096:1138	Imidazole is then emerging as a potential catalyst for the pretreatment of agro-industrial by-products, allowing the valorisation of these residues and their reinsertion into the production chain under a biorefinery concept.					
36693509	3	49	theme	enzymatic	480:488	arg1	efficiency					501:510	enzymatic hydrolysis efficiency	480:510	enzymatic hydrolysis efficiency	480:510	Sugarcane bagasse (SB), soybean hulls (SH), cocoa pod husks (CPH) and oil palm empty fruit bunches (OPEFB) were subjected to imidazole pretreatment in order to evaluate chemical composition variations and influence over enzymatic hydrolysis efficiency.					
36693509	5	50	theme	structural	680:689	arg1	disorganization					691:705	structural disorganization	680:705	structural disorganization of lignocellulosic fibres and enrichment	680:746	After imidazole-pretreatment, all biomasses presented structural disorganization of lignocellulosic fibres and enrichment in the percentage of cellulose.					
35406093	4	0	theme	infiltration	964:975	arg1	symptoms					909:916	the IBD symptoms	901:916	the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues	901:997	In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues.					
35406093	0	1	from	Effect	22:27	arg1	Cells					105:109	LPS-Induced RAW264.7 Macrophages Cells	72:109	LPS-Induced RAW264.7 Macrophages Cells	72:109	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	0	2	dep	Mice	154:157	arg1	Effect					22:27	The Anti-Inflammatory Effect	0:27	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells	0:109	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	0	2	dep	Mice	154:157	arg1	Sulfate					123:129	Dextran Sulfate	115:129	Dextran Sulfate	115:129	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	0	2	dep	Mice	154:157	arg1	Mice					154:157	Sodium-Induced C57BL/6 Mice	131:157	Sodium-Induced C57BL/6 Mice	131:157	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	0	3	theme	Macrophages	93:103	arg1	Cells					105:109	LPS-Induced RAW264.7 Macrophages Cells	72:109	LPS-Induced RAW264.7 Macrophages Cells	72:109	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	2	4	theme	bovine	386:391	arg1	GP					416:417	GP	416:417	GP	416:417	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	2	4	theme	bovine	386:391	arg1	peptides					406:413	bovine bone gelatin peptides	386:413	bovine bone gelatin peptides (GP)	386:418	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	4	5	from	symptoms	909:916	arg1	tissues					991:997	intestinal tissues	980:997	intestinal tissues	980:997	In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues.					
35406093	0	6	theme	Dextran	115:121	arg1	Sulfate					123:129	Dextran Sulfate	115:129	Dextran Sulfate	115:129	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	0	6	theme	Dextran	115:121	arg1	Mice					154:157	Sodium-Induced C57BL/6 Mice	131:157	Sodium-Induced C57BL/6 Mice	131:157	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	2	7	theme	C57BL/6	623:629	arg1	mice					631:634	dextran sodium sulfate (DSS)-induced C57BL/6 mice	586:634	dextran sodium sulfate (DSS)-induced C57BL/6 mice	586:634	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	7	8	theme	DSS-induced	1389:1399	arg1	colitis					1401:1407	DSS-induced colitis	1389:1407	DSS-induced colitis	1389:1407	The above results imply that GP could attenuate DSS-induced colitis by suppressing the inflammatory cytokines and regulating the gut microbiota.					
35406093	3	9	theme	-induced	799:806	arg1	cells					817:821	lipopolysaccharide (LPS)-induced RAW264.7 cells	775:821	lipopolysaccharide (LPS)-induced RAW264.7 cells	775:821	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	2	10	theme	gut	555:557	arg1	compositions					570:581	gut microbiota compositions	555:581	gut microbiota compositions	555:581	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	2	11	theme	-induced	614:621	arg1	mice					631:634	dextran sodium sulfate (DSS)-induced C57BL/6 mice	586:634	dextran sodium sulfate (DSS)-induced C57BL/6 mice	586:634	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	5	12	from	effect	1109:1114	arg1	receptor					1129:1136	toll-like receptor 4	1119:1138	toll-like receptor 4	1119:1138	In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment.					
35406093	5	12	from	effect	1109:1114	arg1	cyclooxygenase-2					1144:1159	cyclooxygenase-2	1144:1159	cyclooxygenase-2	1144:1159	In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment.					
35406093	2	13	theme	bone	393:396	arg1	GP					416:417	GP	416:417	GP	416:417	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	2	13	theme	bone	393:396	arg1	peptides					406:413	bovine bone gelatin peptides	386:413	bovine bone gelatin peptides (GP)	386:418	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	3	14	theme	RAW264.7	808:815	arg1	cells					817:821	lipopolysaccharide (LPS)-induced RAW264.7 cells	775:821	lipopolysaccharide (LPS)-induced RAW264.7 cells	775:821	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	0	15	theme	Sodium-Induced	131:144	arg1	Effect					22:27	The Anti-Inflammatory Effect	0:27	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells	0:109	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	0	15	theme	Sodium-Induced	131:144	arg1	Sulfate					123:129	Dextran Sulfate	115:129	Dextran Sulfate	115:129	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	0	15	theme	Sodium-Induced	131:144	arg1	Mice					154:157	Sodium-Induced C57BL/6 Mice	131:157	Sodium-Induced C57BL/6 Mice	131:157	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	7	16	theme	inflammatory	1428:1439	arg1	cytokines					1441:1449	the inflammatory cytokines	1424:1449	the inflammatory cytokines	1424:1449	The above results imply that GP could attenuate DSS-induced colitis by suppressing the inflammatory cytokines and regulating the gut microbiota.					
35406093	4	17	theme	DSS-induced	827:837	arg1	colitis					839:845	DSS-induced colitis	827:845	DSS-induced colitis mice	827:850	In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues.					
35406093	1	18	theme	bone	199:202	arg1	collagen					204:211	bone collagen	199:211	bone collagen	199:211	The bioactive peptides hydrolyzed from bone collagen have been found to possess health-promoting effects by regulating chronic diseases such as arthritis and hypertension.					
35406093	2	19	theme	animal	477:482	arg1	trials					484:489	animal trials	477:489	animal trials	477:489	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	3	20	theme	oxide	726:730	arg1	secretion					684:692	the extra secretion	674:692	the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells	674:821	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	4	21	theme	intestinal	980:989	arg1	tissues					991:997	intestinal tissues	980:997	intestinal tissues	980:997	In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues.					
35406093	0	22	theme	Anti-Inflammatory	4:20	arg1	Effect					22:27	The Anti-Inflammatory Effect	0:27	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells	0:109	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	0	22	theme	Anti-Inflammatory	4:20	arg1	Mice					154:157	Sodium-Induced C57BL/6 Mice	131:157	Sodium-Induced C57BL/6 Mice	131:157	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	4	23	theme	inflammatory	951:962	arg1	infiltration					964:975	inflammatory infiltration	951:975	inflammatory infiltration	951:975	In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues.					
35406093	6	24	theme	microbiota	1201:1210	arg1	analysis					1185:1192	the analysis	1181:1192	the analysis of gut microbiota	1181:1210	In the analysis of gut microbiota, the GP was checked to modulate the abundance of Akkermansia, Parasutterella, Peptococcus, Bifidobacterium and Saccharibacteria.					
35406093	2	25	theme	inflammatory	528:539	arg1	cytokines					541:549	inflammatory cytokines	528:549	inflammatory cytokines	528:549	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	4	26	theme	weight	921:926	arg1	loss					928:931	weight loss	921:931	weight loss	921:931	In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues.					
35406093	3	27	theme	necrosis	747:754	arg1	TNF-α					765:769	TNF-α	765:769	TNF-α	765:769	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	3	27	theme	necrosis	747:754	arg1	factor-α					756:763	tumor necrosis factor-α	741:763	tumor necrosis factor-α(TNF-α)	741:770	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	4	28	theme	colitis	839:845	arg1	mice					847:850	DSS-induced colitis mice	827:850	DSS-induced colitis mice	827:850	In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues.					
35406093	5	29	theme	proinflammatory	1014:1028	arg1	cytokines					1030:1038	the proinflammatory cytokines	1010:1038	the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β)	1010:1065	In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment.					
35406093	6	30	theme	Parasutterella	1274:1287	arg1	abundance					1248:1256	the abundance	1244:1256	the abundance of Akkermansia, Parasutterella, Peptococcus, Bifidobacterium and Saccharibacteria	1244:1338	In the analysis of gut microbiota, the GP was checked to modulate the abundance of Akkermansia, Parasutterella, Peptococcus, Bifidobacterium and Saccharibacteria.					
35406093	3	31	theme	factor-α	756:763	arg1	secretion					684:692	the extra secretion	674:692	the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells	674:821	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	4	32	theme	loss	928:931	arg1	symptoms					909:916	the IBD symptoms	901:916	the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues	901:997	In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues.					
35406093	6	33	theme	Peptococcus	1290:1300	arg1	abundance					1248:1256	the abundance	1244:1256	the abundance of Akkermansia, Parasutterella, Peptococcus, Bifidobacterium and Saccharibacteria	1244:1338	In the analysis of gut microbiota, the GP was checked to modulate the abundance of Akkermansia, Parasutterella, Peptococcus, Bifidobacterium and Saccharibacteria.					
35406093	2	34	theme	current	339:345	arg1	study					347:351	the current study	335:351	the current study	335:351	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	2	35	theme	macrophages	443:453	arg1	cells					455:459	264.7 macrophages cells	437:459	264.7 macrophages cells	437:459	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	0	36	theme	C57BL/6	146:152	arg1	Effect					22:27	The Anti-Inflammatory Effect	0:27	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells	0:109	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	0	36	theme	C57BL/6	146:152	arg1	Sulfate					123:129	Dextran Sulfate	115:129	Dextran Sulfate	115:129	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	0	36	theme	C57BL/6	146:152	arg1	Mice					154:157	Sodium-Induced C57BL/6 Mice	131:157	Sodium-Induced C57BL/6 Mice	131:157	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	2	37	from	interference	512:523	arg1	mice					631:634	dextran sodium sulfate (DSS)-induced C57BL/6 mice	586:634	dextran sodium sulfate (DSS)-induced C57BL/6 mice	586:634	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	2	37	from	interference	512:523	arg1	compositions					570:581	gut microbiota compositions	555:581	gut microbiota compositions	555:581	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	2	37	from	interference	512:523	arg1	cytokines					541:549	inflammatory cytokines	528:549	inflammatory cytokines	528:549	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	5	38	dep	cytokines	1030:1038	arg1	TNF-α					1041:1045	TNF-α	1041:1045	TNF-α	1041:1045	In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment.					
35406093	2	39	theme	peptides	406:413	arg1	effect					376:381	the anti-inflammatory effect	354:381	the anti-inflammatory effect of bovine bone gelatin peptides (GP)	354:418	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	0	40	theme	Bone-Gelatin-Derived	39:58	arg1	Peptides					60:67	Bovine Bone-Gelatin-Derived Peptides	32:67	Bovine Bone-Gelatin-Derived Peptides	32:67	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	3	41	theme	tumor	741:745	arg1	TNF-α					765:769	TNF-α	765:769	TNF-α	765:769	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	3	41	theme	tumor	741:745	arg1	factor-α					756:763	tumor necrosis factor-α	741:763	tumor necrosis factor-α(TNF-α)	741:770	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	3	42	theme	extra	678:682	arg1	secretion					684:692	the extra secretion	674:692	the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells	674:821	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	5	43	theme	GP	1164:1165	arg1	treatment					1167:1175	GP treatment	1164:1175	GP treatment	1164:1175	In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment.					
35406093	0	44	theme	Bovine	32:37	arg1	Peptides					60:67	Bovine Bone-Gelatin-Derived Peptides	32:67	Bovine Bone-Gelatin-Derived Peptides	32:67	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	5	45	theme	decreasing	1098:1107	arg1	effect					1109:1114	the decreasing effect	1094:1114	the decreasing effect on toll-like receptor 4 and cyclooxygenase-2	1094:1159	In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment.					
35406093	4	46	theme	IBD	905:907	arg1	symptoms					909:916	the IBD symptoms	901:916	the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues	901:997	In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues.					
35406093	7	47	theme	above	1345:1349	arg1	results					1351:1357	The above results	1341:1357	The above results	1341:1357	The above results imply that GP could attenuate DSS-induced colitis by suppressing the inflammatory cytokines and regulating the gut microbiota.					
35406093	1	48	theme	health-promoting	240:255	arg1	effects					257:263	health-promoting effects	240:263	health-promoting effects	240:263	The bioactive peptides hydrolyzed from bone collagen have been found to possess health-promoting effects by regulating chronic diseases such as arthritis and hypertension.					
35406093	2	49	theme	microbiota	559:568	arg1	compositions					570:581	gut microbiota compositions	555:581	gut microbiota compositions	555:581	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	4	50	theme	hematochezia	934:945	arg1	symptoms					909:916	the IBD symptoms	901:916	the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues	901:997	In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues.					
35406093	0	51	theme	Peptides	60:67	arg1	Effect					22:27	The Anti-Inflammatory Effect	0:27	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells	0:109	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	0	51	theme	Peptides	60:67	arg1	Sulfate					123:129	Dextran Sulfate	115:129	Dextran Sulfate	115:129	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	0	51	theme	Peptides	60:67	arg1	Mice					154:157	Sodium-Induced C57BL/6 Mice	131:157	Sodium-Induced C57BL/6 Mice	131:157	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	4	52	theme	GP	867:868	arg1	gavage					857:862	the gavage	853:862	the gavage of GP	853:868	In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues.					
35406093	3	53	theme	interleukin-6	697:709	arg1	secretion					684:692	the extra secretion	674:692	the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells	674:821	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	1	54	contain	possess	232:238	arg1	peptides					174:181	The bioactive peptides	160:181	The bioactive peptides hydrolyzed from bone collagen	160:211	The bioactive peptides hydrolyzed from bone collagen have been found to possess health-promoting effects by regulating chronic diseases such as arthritis and hypertension.					
35406093	1	54	contain	possess	232:238	arg2	effects					257:263	health-promoting effects	240:263	health-promoting effects	240:263	The bioactive peptides hydrolyzed from bone collagen have been found to possess health-promoting effects by regulating chronic diseases such as arthritis and hypertension.					
35406093	5	55	theme	toll-like	1119:1127	arg1	receptor					1129:1136	toll-like receptor 4	1119:1138	toll-like receptor 4	1119:1138	In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment.					
35406093	5	56	dep	TNF-α	1041:1045	arg1	IL-6					1047:1050	IL-6	1047:1050	IL-6	1047:1050	In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment.					
35406093	5	56	dep	TNF-α	1041:1045	arg1	IL-1β					1060:1064	IL-1β	1060:1064	IL-1β	1060:1064	In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment.					
35406093	5	56	dep	TNF-α	1041:1045	arg1	MCP-1					1053:1057	MCP-1	1053:1057	MCP-1	1053:1057	In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment.					
35406093	0	57	from	Sulfate	123:129	arg1	Cells					105:109	LPS-Induced RAW264.7 Macrophages Cells	72:109	LPS-Induced RAW264.7 Macrophages Cells	72:109	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	3	58	from	secretion	684:692	arg1	cells					817:821	lipopolysaccharide (LPS)-induced RAW264.7 cells	775:821	lipopolysaccharide (LPS)-induced RAW264.7 cells	775:821	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	2	59	theme	anti-inflammatory	358:374	arg1	effect					376:381	the anti-inflammatory effect	354:381	the anti-inflammatory effect of bovine bone gelatin peptides (GP)	354:418	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	7	60	theme	gut	1470:1472	arg1	microbiota					1474:1483	the gut microbiota	1466:1483	the gut microbiota	1466:1483	The above results imply that GP could attenuate DSS-induced colitis by suppressing the inflammatory cytokines and regulating the gut microbiota.					
35406093	6	61	theme	Bifidobacterium	1303:1317	arg1	abundance					1248:1256	the abundance	1244:1256	the abundance of Akkermansia, Parasutterella, Peptococcus, Bifidobacterium and Saccharibacteria	1244:1338	In the analysis of gut microbiota, the GP was checked to modulate the abundance of Akkermansia, Parasutterella, Peptococcus, Bifidobacterium and Saccharibacteria.					
35406093	0	62	theme	RAW264.7	84:91	arg1	Macrophages					93:103	LPS-Induced RAW264.7 Macrophages	72:103	LPS-Induced RAW264.7 Macrophages Cells	72:109	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	6	63	theme	Saccharibacteria	1323:1338	arg1	abundance					1248:1256	the abundance	1244:1256	the abundance of Akkermansia, Parasutterella, Peptococcus, Bifidobacterium and Saccharibacteria	1244:1338	In the analysis of gut microbiota, the GP was checked to modulate the abundance of Akkermansia, Parasutterella, Peptococcus, Bifidobacterium and Saccharibacteria.					
35406093	1	64	theme	bioactive	164:172	arg1	peptides					174:181	The bioactive peptides	160:181	The bioactive peptides hydrolyzed from bone collagen	160:211	The bioactive peptides hydrolyzed from bone collagen have been found to possess health-promoting effects by regulating chronic diseases such as arthritis and hypertension.					
35406093	0	65	theme	LPS-Induced	72:82	arg1	Macrophages					93:103	LPS-Induced RAW264.7 Macrophages	72:103	LPS-Induced RAW264.7 Macrophages Cells	72:109	The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice.					
35406093	6	66	theme	gut	1197:1199	arg1	microbiota					1201:1210	gut microbiota	1197:1210	gut microbiota	1197:1210	In the analysis of gut microbiota, the GP was checked to modulate the abundance of Akkermansia, Parasutterella, Peptococcus, Bifidobacterium and Saccharibacteria.					
35406093	3	67	theme	nitric	719:724	arg1	NO					733:734	NO	733:734	NO	733:734	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	3	67	theme	nitric	719:724	arg1	oxide					726:730	nitric oxide	719:730	nitric oxide (NO)	719:735	The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells.					
35406093	2	68	theme	gelatin	398:404	arg1	GP					416:417	GP	416:417	GP	416:417	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	2	68	theme	gelatin	398:404	arg1	peptides					406:413	bovine bone gelatin peptides	386:413	bovine bone gelatin peptides (GP)	386:418	In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice.					
35406093	1	69	theme	chronic	279:285	arg1	hypertension					318:329	hypertension	318:329	hypertension	318:329	The bioactive peptides hydrolyzed from bone collagen have been found to possess health-promoting effects by regulating chronic diseases such as arthritis and hypertension.					
35406093	1	69	theme	chronic	279:285	arg1	diseases					287:294	chronic diseases	279:294	chronic diseases such as arthritis and hypertension	279:329	The bioactive peptides hydrolyzed from bone collagen have been found to possess health-promoting effects by regulating chronic diseases such as arthritis and hypertension.					
35406093	1	69	theme	chronic	279:285	arg1	arthritis					304:312	arthritis	304:312	arthritis	304:312	The bioactive peptides hydrolyzed from bone collagen have been found to possess health-promoting effects by regulating chronic diseases such as arthritis and hypertension.					
36282710	7	0	theme	lipid-rich	1159:1168	arg1	particles					1170:1178	these lipid-rich particles	1153:1178	these lipid-rich particles	1153:1178	Isolation relies on the low density of these lipid-rich particles, which facilitates their purification by sucrose density flotation.					
36282710	5	1	from	chloroplasts	821:832	arg1	isolation					788:796	the isolation	784:796	the isolation of plastoglobules from chloroplasts of plant leaf tissue	784:853	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	1	from	chloroplasts	821:832	arg1	plastoglobules					801:814	plastoglobules	801:814	plastoglobules from chloroplasts of plant leaf tissue	801:853	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	1	2	theme	cyanobacteria	165:177	arg1	droplets					100:107	Plastoglobule lipid droplets	80:107	Plastoglobule lipid droplets	80:107	Plastoglobule lipid droplets are a dynamic sub-compartment of plant chloroplasts and cyanobacteria.					
36282710	1	2	theme	cyanobacteria	165:177	arg1	sub-compartment					123:137	a dynamic sub-compartment	113:137	a dynamic sub-compartment of plant chloroplasts and cyanobacteria	113:177	Plastoglobule lipid droplets are a dynamic sub-compartment of plant chloroplasts and cyanobacteria.					
36282710	5	3	theme	plastoglobules	801:814	arg1	isolation					788:796	the isolation	784:796	the isolation of plastoglobules from chloroplasts of plant leaf tissue	784:853	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	4	4	theme	purity	552:557	arg1	plastoglobules					529:542	plastoglobules	529:542	plastoglobules of high purity and yield	529:567	With plastoglobules of high purity and yield, it is possible to investigate their lipid and protein composition, enzymatic activity, and protein topology, among other possible molecular characteristics.					
36282710	7	5	theme	particles	1170:1178	arg1	density					1142:1148	the low density	1134:1148	the low density	1134:1148	Isolation relies on the low density of these lipid-rich particles, which facilitates their purification by sucrose density flotation.					
36282710	5	6	theme	structures	956:965	arg1	isolation					902:910	the isolation	898:910	the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp	898:1101	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	4	7	theme	high	547:550	arg1	purity					552:557	high purity	547:557	high purity	547:557	With plastoglobules of high purity and yield, it is possible to investigate their lipid and protein composition, enzymatic activity, and protein topology, among other possible molecular characteristics.					
36282710	3	8	theme	high	421:424	arg1	purity					426:431	high purity	421:431	high purity	421:431	The capacity to isolate plastoglobules of high purity has greatly facilitated their study through proteomic, lipidomic, and other methodologies.					
36282710	5	9	theme	lipid	942:946	arg1	structures					956:965	related lipid droplet structures	934:965	related lipid droplet structures	934:965	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	4	10	theme	protein	616:622	arg1	composition					624:634	their lipid and protein composition	600:634	composition	624:634	With plastoglobules of high purity and yield, it is possible to investigate their lipid and protein composition, enzymatic activity, and protein topology, among other possible molecular characteristics.					
36282710	5	11	theme	effective	761:769	arg1	protocol					771:778	a rapid and effective protocol	749:778	a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue	749:853	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	0	12	from	Tissue	54:59	arg1	Isolation					28:36	Isolation	28:36	Isolation	28:36	Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria.					
36282710	5	13	theme	droplet	948:954	arg1	structures					956:965	related lipid droplet structures	934:965	related lipid droplet structures	934:965	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	14	dep	sp	1100:1101	arg1	Synechocystis					1086:1098	the cyanobacterium, Synechocystis sp	1066:1101	Synechocystis	1086:1098	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	7	15	theme	low	1138:1140	arg1	density					1142:1148	the low density	1134:1148	the low density	1134:1148	Isolation relies on the low density of these lipid-rich particles, which facilitates their purification by sucrose density flotation.					
36282710	5	16	theme	leaf	843:846	arg1	tissue					848:853	plant leaf tissue	837:853	plant leaf tissue	837:853	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	4	17	with	possible	576:583	arg1	plastoglobules					529:542	plastoglobules	529:542	plastoglobules of high purity and yield	529:567	With plastoglobules of high purity and yield, it is possible to investigate their lipid and protein composition, enzymatic activity, and protein topology, among other possible molecular characteristics.					
36282710	5	18	theme	plastoglobules	915:928	arg1	isolation					902:910	the isolation	898:910	the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp	898:1101	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	19	theme	plant	837:841	arg1	tissue					848:853	plant leaf tissue	837:853	plant leaf tissue	837:853	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	20	dep	maize	972:976	arg1	nindensis					1051:1059	Eragrostis nindensis	1040:1059	Eragrostis nindensis	1040:1059	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	20	dep	maize	972:976	arg1	tissue					1006:1011	the desiccated leaf tissue	986:1011	the desiccated leaf tissue of the resurrection plant	986:1037	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	20	dep	maize	972:976	arg1	leaves					978:983	leaves	978:983	leaves	978:983	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	20	dep	maize	972:976	arg1	sp					1100:1101	the cyanobacterium, Synechocystis sp	1066:1101	sp	1100:1101	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	1	21	theme	Plastoglobule	80:92	arg1	droplets					100:107	Plastoglobule lipid droplets	80:107	Plastoglobule lipid droplets	80:107	Plastoglobule lipid droplets are a dynamic sub-compartment of plant chloroplasts and cyanobacteria.					
36282710	1	21	theme	Plastoglobule	80:92	arg1	sub-compartment					123:137	a dynamic sub-compartment	113:137	a dynamic sub-compartment of plant chloroplasts and cyanobacteria	113:177	Plastoglobule lipid droplets are a dynamic sub-compartment of plant chloroplasts and cyanobacteria.					
36282710	3	22	theme	proteomic	477:485	arg1	methodologies					509:521	proteomic, lipidomic, and other methodologies	477:521	proteomic, lipidomic, and other methodologies	477:521	The capacity to isolate plastoglobules of high purity has greatly facilitated their study through proteomic, lipidomic, and other methodologies.					
36282710	0	23	theme	Lipid	14:18	arg1	Droplet					20:26	Plastoglobule Lipid Droplet	0:26	Plastoglobule Lipid Droplet	0:26	Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria.					
36282710	8	24	theme	plastoglobules	1301:1314	arg1	study					1292:1296	the study	1288:1296	the study of plastoglobules from diverse species	1288:1335	This methodology will prove valuable in the study of plastoglobules from diverse species.					
36282710	1	25	theme	lipid	94:98	arg1	droplets					100:107	Plastoglobule lipid droplets	80:107	Plastoglobule lipid droplets	80:107	Plastoglobule lipid droplets are a dynamic sub-compartment of plant chloroplasts and cyanobacteria.					
36282710	1	25	theme	lipid	94:98	arg1	sub-compartment					123:137	a dynamic sub-compartment	113:137	a dynamic sub-compartment of plant chloroplasts and cyanobacteria	113:177	Plastoglobule lipid droplets are a dynamic sub-compartment of plant chloroplasts and cyanobacteria.					
36282710	7	26	theme	sucrose	1221:1227	arg1	flotation					1237:1245	sucrose density flotation	1221:1245	sucrose density flotation	1221:1245	Isolation relies on the low density of these lipid-rich particles, which facilitates their purification by sucrose density flotation.					
36282710	4	27	theme	possible	691:698	arg1	characteristics					710:724	other possible molecular characteristics	685:724	other possible molecular characteristics	685:724	With plastoglobules of high purity and yield, it is possible to investigate their lipid and protein composition, enzymatic activity, and protein topology, among other possible molecular characteristics.					
36282710	0	28	theme	Plastoglobule	0:12	arg1	Droplet					20:26	Plastoglobule Lipid Droplet	0:26	Plastoglobule Lipid Droplet	0:26	Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria.					
36282710	5	29	from	isolation	788:796	arg1	chloroplasts					821:832	chloroplasts	821:832	chloroplasts of plant leaf tissue	821:853	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	2	30	theme	membrane	321:328	arg1	remodeling					293:302	remodeling	293:302	remodeling	293:302	Found ubiquitously among photosynthetic species, they are believed to serve a central role in the adaptation and remodeling of the thylakoid membrane under rapidly changing environmental conditions.					
36282710	2	30	theme	membrane	321:328	arg1	adaptation					278:287	adaptation	278:287	adaptation	278:287	Found ubiquitously among photosynthetic species, they are believed to serve a central role in the adaptation and remodeling of the thylakoid membrane under rapidly changing environmental conditions.					
36282710	2	31	from	role	266:269	arg1	remodeling					293:302	remodeling	293:302	remodeling	293:302	Found ubiquitously among photosynthetic species, they are believed to serve a central role in the adaptation and remodeling of the thylakoid membrane under rapidly changing environmental conditions.					
36282710	2	31	from	role	266:269	arg1	adaptation					278:287	adaptation	278:287	adaptation	278:287	Found ubiquitously among photosynthetic species, they are believed to serve a central role in the adaptation and remodeling of the thylakoid membrane under rapidly changing environmental conditions.					
36282710	4	32	theme	other	685:689	arg1	characteristics					710:724	other possible molecular characteristics	685:724	other possible molecular characteristics	685:724	With plastoglobules of high purity and yield, it is possible to investigate their lipid and protein composition, enzymatic activity, and protein topology, among other possible molecular characteristics.					
36282710	5	33	theme	tissue	848:853	arg1	chloroplasts					821:832	chloroplasts	821:832	chloroplasts of plant leaf tissue	821:853	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	34	from	maize	972:976	arg1	structures					956:965	related lipid droplet structures	934:965	related lipid droplet structures	934:965	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	34	from	maize	972:976	arg1	plastoglobules					915:928	plastoglobules	915:928	plastoglobules	915:928	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	34	from	maize	972:976	arg1	isolation					902:910	the isolation	898:910	the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp	898:1101	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	2	35	theme	thylakoid	311:319	arg1	membrane					321:328	the thylakoid membrane	307:328	the thylakoid membrane	307:328	Found ubiquitously among photosynthetic species, they are believed to serve a central role in the adaptation and remodeling of the thylakoid membrane under rapidly changing environmental conditions.					
36282710	5	36	theme	methodological	868:881	arg1	variations					883:892	methodological variations	868:892	methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp	868:1101	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	37	theme	desiccated	990:999	arg1	tissue					1006:1011	the desiccated leaf tissue	986:1011	the desiccated leaf tissue of the resurrection plant	986:1037	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	38	theme	resurrection	1020:1031	arg1	plant					1033:1037	the resurrection plant	1016:1037	the resurrection plant	1016:1037	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	2	39	theme	photosynthetic	205:218	arg1	species					220:226	photosynthetic species	205:226	photosynthetic species	205:226	Found ubiquitously among photosynthetic species, they are believed to serve a central role in the adaptation and remodeling of the thylakoid membrane under rapidly changing environmental conditions.					
36282710	8	40	from	species	1329:1335	arg1	plastoglobules					1301:1314	plastoglobules	1301:1314	plastoglobules from diverse species	1301:1335	This methodology will prove valuable in the study of plastoglobules from diverse species.					
36282710	8	40	from	species	1329:1335	arg1	study					1292:1296	the study	1288:1296	the study of plastoglobules from diverse species	1288:1335	This methodology will prove valuable in the study of plastoglobules from diverse species.					
36282710	5	41	theme	related	934:940	arg1	structures					956:965	related lipid droplet structures	934:965	related lipid droplet structures	934:965	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	0	42	theme	Plant	43:47	arg1	Tissue					54:59	Plant Leaf Tissue	43:59	Plant Leaf Tissue	43:59	Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria.					
36282710	7	43	theme	density	1229:1235	arg1	flotation					1237:1245	sucrose density flotation	1221:1245	sucrose density flotation	1221:1245	Isolation relies on the low density of these lipid-rich particles, which facilitates their purification by sucrose density flotation.					
36282710	4	44	theme	yield	563:567	arg1	plastoglobules					529:542	plastoglobules	529:542	plastoglobules of high purity and yield	529:567	With plastoglobules of high purity and yield, it is possible to investigate their lipid and protein composition, enzymatic activity, and protein topology, among other possible molecular characteristics.					
36282710	5	45	theme	rapid	751:755	arg1	protocol					771:778	a rapid and effective protocol	749:778	a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue	749:853	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	0	46	from	Cyanobacteria	65:77	arg1	Isolation					28:36	Isolation	28:36	Isolation	28:36	Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria.					
36282710	3	47	theme	lipidomic	488:496	arg1	methodologies					509:521	proteomic, lipidomic, and other methodologies	477:521	proteomic, lipidomic, and other methodologies	477:521	The capacity to isolate plastoglobules of high purity has greatly facilitated their study through proteomic, lipidomic, and other methodologies.					
36282710	3	48	theme	purity	426:431	arg1	plastoglobules					403:416	plastoglobules	403:416	plastoglobules of high purity	403:431	The capacity to isolate plastoglobules of high purity has greatly facilitated their study through proteomic, lipidomic, and other methodologies.					
36282710	5	49	theme	cyanobacterium	1070:1083	arg1	sp					1100:1101	the cyanobacterium, Synechocystis sp	1066:1101	sp	1100:1101	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	1	50	theme	dynamic	115:121	arg1	droplets					100:107	Plastoglobule lipid droplets	80:107	Plastoglobule lipid droplets	80:107	Plastoglobule lipid droplets are a dynamic sub-compartment of plant chloroplasts and cyanobacteria.					
36282710	1	50	theme	dynamic	115:121	arg1	sub-compartment					123:137	a dynamic sub-compartment	113:137	a dynamic sub-compartment of plant chloroplasts and cyanobacteria	113:177	Plastoglobule lipid droplets are a dynamic sub-compartment of plant chloroplasts and cyanobacteria.					
36282710	0	51	theme	Leaf	49:52	arg1	Tissue					54:59	Plant Leaf Tissue	43:59	Plant Leaf Tissue	43:59	Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria.					
36282710	8	52	from	study	1292:1296	arg1	species					1329:1335	diverse species	1321:1335	diverse species	1321:1335	This methodology will prove valuable in the study of plastoglobules from diverse species.					
36282710	2	53	theme	environmental	353:365	arg1	conditions					367:376	rapidly changing environmental conditions	336:376	rapidly changing environmental conditions	336:376	Found ubiquitously among photosynthetic species, they are believed to serve a central role in the adaptation and remodeling of the thylakoid membrane under rapidly changing environmental conditions.					
36282710	4	54	theme	molecular	700:708	arg1	characteristics					710:724	other possible molecular characteristics	685:724	other possible molecular characteristics	685:724	With plastoglobules of high purity and yield, it is possible to investigate their lipid and protein composition, enzymatic activity, and protein topology, among other possible molecular characteristics.					
36282710	2	55	theme	changing	344:351	arg1	conditions					367:376	rapidly changing environmental conditions	336:376	rapidly changing environmental conditions	336:376	Found ubiquitously among photosynthetic species, they are believed to serve a central role in the adaptation and remodeling of the thylakoid membrane under rapidly changing environmental conditions.					
36282710	5	56	theme	leaf	1001:1004	arg1	tissue					1006:1011	the desiccated leaf tissue	986:1011	the desiccated leaf tissue of the resurrection plant	986:1037	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	57	theme	plant	1033:1037	arg1	tissue					1006:1011	the desiccated leaf tissue	986:1011	the desiccated leaf tissue of the resurrection plant	986:1037	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	5	58	theme	Eragrostis	1040:1049	arg1	nindensis					1051:1059	Eragrostis nindensis	1040:1059	Eragrostis nindensis	1040:1059	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	1	59	theme	plant	142:146	arg1	chloroplasts					148:159	plant chloroplasts	142:159	plant chloroplasts	142:159	Plastoglobule lipid droplets are a dynamic sub-compartment of plant chloroplasts and cyanobacteria.					
36282710	3	60	theme	other	503:507	arg1	methodologies					509:521	proteomic, lipidomic, and other methodologies	477:521	proteomic, lipidomic, and other methodologies	477:521	The capacity to isolate plastoglobules of high purity has greatly facilitated their study through proteomic, lipidomic, and other methodologies.					
36282710	4	61	theme	protein	661:667	arg1	topology					669:676	protein topology	661:676	protein topology	661:676	With plastoglobules of high purity and yield, it is possible to investigate their lipid and protein composition, enzymatic activity, and protein topology, among other possible molecular characteristics.					
36282710	8	62	theme	diverse	1321:1327	arg1	species					1329:1335	diverse species	1321:1335	diverse species	1321:1335	This methodology will prove valuable in the study of plastoglobules from diverse species.					
36282710	1	63	theme	chloroplasts	148:159	arg1	droplets					100:107	Plastoglobule lipid droplets	80:107	Plastoglobule lipid droplets	80:107	Plastoglobule lipid droplets are a dynamic sub-compartment of plant chloroplasts and cyanobacteria.					
36282710	1	63	theme	chloroplasts	148:159	arg1	sub-compartment					123:137	a dynamic sub-compartment	113:137	a dynamic sub-compartment of plant chloroplasts and cyanobacteria	113:177	Plastoglobule lipid droplets are a dynamic sub-compartment of plant chloroplasts and cyanobacteria.					
36282710	2	64	theme	central	258:264	arg1	role					266:269	a central role	256:269	a central role in the adaptation and remodeling of the thylakoid membrane	256:328	Found ubiquitously among photosynthetic species, they are believed to serve a central role in the adaptation and remodeling of the thylakoid membrane under rapidly changing environmental conditions.					
36282710	5	65	from	isolation	902:910	arg1	maize					972:976	maize	972:976	maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp	972:1101	This article presents a rapid and effective protocol for the isolation of plastoglobules from chloroplasts of plant leaf tissue and presents methodological variations for the isolation of plastoglobules and related lipid droplet structures from maize leaves, the desiccated leaf tissue of the resurrection plant, Eragrostis nindensis, and the cyanobacterium, Synechocystis sp.					
36282710	2	66	dep	adaptation	278:287	arg1	the					274:276	the	274:276	the	274:276	Found ubiquitously among photosynthetic species, they are believed to serve a central role in the adaptation and remodeling of the thylakoid membrane under rapidly changing environmental conditions.					
36282710	4	67	theme	enzymatic	637:645	arg1	activity					647:654	enzymatic activity	637:654	enzymatic activity	637:654	With plastoglobules of high purity and yield, it is possible to investigate their lipid and protein composition, enzymatic activity, and protein topology, among other possible molecular characteristics.					
37394217	7	0	theme	GIT	1484:1486	arg1	environment					1488:1498	the GIT environment	1480:1498	the GIT environment	1480:1498	The results of study will help to design lactic acid bacteria encapsulation systems based on the GIT environment for the oral delivery strategy.					
37394217	4	1	theme	emulsion	741:748	arg1	size					729:732	the particle size	716:732	the particle size of the emulsion	716:748	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	3	2	theme	O/W	450:452	arg1	emulsions					479:487	the O/W (Oil-in-water emulsions) emulsions	446:487	the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation	446:598	This study investigated the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation, and the stability of the strains during gastrointestinal environment were also determined.					
37394217	3	2	theme	O/W	450:452	arg1	emulsions					468:476	Oil-in-water emulsions	455:476	Oil-in-water emulsions	455:476	This study investigated the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation, and the stability of the strains during gastrointestinal environment were also determined.					
37394217	2	3	theme	food	377:380	arg1	processing					382:391	food processing	377:391	food processing	377:391	However, the viability of probiotics can be affected by food processing and the adverse environment.					
37394217	6	4	from	35 days	1359:1365	arg1	4 °C					1381:1384	4 °C	1381:1384	4 °C	1381:1384	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	0	5	theme	plantarum	91:99	arg1	survival					59:66	the survival	55:66	the survival of Lactiplantibacillus plantarum in the stimulated gastrointestinal conditions	55:145	Enhancement of gum Arabic/casein microencapsulation on the survival of Lactiplantibacillus plantarum in the stimulated gastrointestinal conditions.					
37394217	6	6	theme	plantarum	1303:1311	arg1	activity					1288:1295	the activity	1284:1295	the activity of L. plantarum	1284:1311	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	6	6	theme	plantarum	1303:1311	arg1	CFU/mL					1344:1349	about 7.51 log CFU/mL	1329:1349	about 7.51 log CFU/mL	1329:1349	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	6	6	theme	plantarum	1303:1311	arg1	stable					1321:1326	stable	1321:1326	stable	1321:1326	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	1	7	theme	long	198:201	arg1	histories					203:211	long histories	198:211	long histories of safe use	198:223	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	1	8	theme	gastrointestinal	291:306	arg1	GIT					315:317	GIT	315:317	GIT	315:317	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	1	8	theme	gastrointestinal	291:306	arg1	tract					308:312	the gastrointestinal tract	287:312	the gastrointestinal tract (GIT)	287:318	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	0	9	theme	stimulated	108:117	arg1	conditions					136:145	the stimulated gastrointestinal conditions	104:145	the stimulated gastrointestinal conditions	104:145	Enhancement of gum Arabic/casein microencapsulation on the survival of Lactiplantibacillus plantarum in the stimulated gastrointestinal conditions.					
37394217	0	10	from	Enhancement	0:10	arg1	survival					59:66	the survival	55:66	the survival of Lactiplantibacillus plantarum in the stimulated gastrointestinal conditions	55:145	Enhancement of gum Arabic/casein microencapsulation on the survival of Lactiplantibacillus plantarum in the stimulated gastrointestinal conditions.					
37394217	3	11	theme	plantarum	571:579	arg1	microencapsulation					581:598	Lactiplantibacillus plantarum microencapsulation	551:598	Lactiplantibacillus plantarum microencapsulation	551:598	This study investigated the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation, and the stability of the strains during gastrointestinal environment were also determined.					
37394217	0	12	from	survival	59:66	arg1	conditions					136:145	the stimulated gastrointestinal conditions	104:145	the stimulated gastrointestinal conditions	104:145	Enhancement of gum Arabic/casein microencapsulation on the survival of Lactiplantibacillus plantarum in the stimulated gastrointestinal conditions.					
37394217	6	13	theme	higher	1204:1209	arg1	count					1218:1222	a higher viable count	1202:1222	a higher viable count	1202:1222	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	3	14	theme	Gum	525:527	arg1	complexes					537:545	casein/GA (Gum Arabic) complexes	514:545	casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation	514:598	This study investigated the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation, and the stability of the strains during gastrointestinal environment were also determined.					
37394217	4	15	dep	5.48 μm	776:782	arg1	to					773:774	to	773:774	to	773:774	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	1	16	contain	have	193:196	arg1	products					158:165	Probiotic products	148:165	Probiotic products that contain lactobacilli	148:191	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	1	16	contain	have	193:196	arg2	histories					203:211	long histories	198:211	long histories of safe use	198:223	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	5	17	dep	smooth	1018:1023	arg1	dense					1026:1030	dense	1026:1030	dense	1026:1030	The surface of this microencapsulated casein/GA composite forms smooth, dense agglomerates and has high viscoelasticity, which effectively improved casein's emulsifying activity (8.66 ± 0.17 m2/g).					
37394217	1	18	theme	safe	216:219	arg1	use					221:223	safe use	216:223	safe use	216:223	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	3	19	theme	Oil-in-water	455:466	arg1	emulsions					479:487	the O/W (Oil-in-water emulsions) emulsions	446:487	the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation	446:598	This study investigated the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation, and the stability of the strains during gastrointestinal environment were also determined.					
37394217	3	19	theme	Oil-in-water	455:466	arg1	emulsions					468:476	Oil-in-water emulsions	455:476	Oil-in-water emulsions	455:476	This study investigated the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation, and the stability of the strains during gastrointestinal environment were also determined.					
37394217	0	20	theme	gastrointestinal	119:134	arg1	conditions					136:145	the stimulated gastrointestinal conditions	104:145	the stimulated gastrointestinal conditions	104:145	Enhancement of gum Arabic/casein microencapsulation on the survival of Lactiplantibacillus plantarum in the stimulated gastrointestinal conditions.					
37394217	7	21	theme	acid	1435:1438	arg1	bacteria					1440:1447	lactic acid bacteria	1428:1447	lactic acid bacteria	1428:1447	The results of study will help to design lactic acid bacteria encapsulation systems based on the GIT environment for the oral delivery strategy.					
37394217	4	22	theme	emulsion	847:854	arg1	particles					856:864	the emulsion particles	843:864	the emulsion particles	843:864	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	4	22	theme	emulsion	847:854	arg1	uniform					888:894	uniform	888:894	uniform	888:894	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	3	23	theme	Lactiplantibacillus	551:569	arg1	microencapsulation					581:598	Lactiplantibacillus plantarum microencapsulation	551:598	Lactiplantibacillus plantarum microencapsulation	551:598	This study investigated the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation, and the stability of the strains during gastrointestinal environment were also determined.					
37394217	6	24	dep	stable	1321:1326	arg1	activity					1288:1295	the activity	1284:1295	the activity of L. plantarum	1284:1311	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	6	24	dep	stable	1321:1326	arg1	CFU/mL					1344:1349	about 7.51 log CFU/mL	1329:1349	about 7.51 log CFU/mL	1329:1349	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	6	24	dep	stable	1321:1326	arg1	stable					1321:1326	stable	1321:1326	stable	1321:1326	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	1	25	contain	contain	172:178	arg1	products					158:165	Probiotic products	148:165	Probiotic products that contain lactobacilli	148:191	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	1	25	contain	contain	172:178	arg2	lactobacilli					180:191	lactobacilli	180:191	lactobacilli	180:191	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	7	26	theme	study	1402:1406	arg1	results					1391:1397	The results	1387:1397	The results of study	1387:1406	The results of study will help to design lactic acid bacteria encapsulation systems based on the GIT environment for the oral delivery strategy.					
37394217	5	27	theme	emulsifying	1111:1121	arg1	activity					1123:1130	casein's emulsifying activity	1102:1130	casein's emulsifying activity (8.66 ± 0.17 m2/g)	1102:1149	The surface of this microencapsulated casein/GA composite forms smooth, dense agglomerates and has high viscoelasticity, which effectively improved casein's emulsifying activity (8.66 ± 0.17 m2/g).					
37394217	5	27	theme	emulsifying	1111:1121	arg1	8.66 ± 0.17 m2/g					1133:1148	8.66 ± 0.17 m2/g	1133:1148	8.66 ± 0.17 m2/g	1133:1148	The surface of this microencapsulated casein/GA composite forms smooth, dense agglomerates and has high viscoelasticity, which effectively improved casein's emulsifying activity (8.66 ± 0.17 m2/g).					
37394217	2	28	theme	adverse	401:407	arg1	environment					409:419	the adverse environment	397:419	the adverse environment	397:419	However, the viability of probiotics can be affected by food processing and the adverse environment.					
37394217	6	29	theme	log	1340:1342	arg1	activity					1288:1295	the activity	1284:1295	the activity of L. plantarum	1284:1311	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	6	29	theme	log	1340:1342	arg1	CFU/mL					1344:1349	about 7.51 log CFU/mL	1329:1349	about 7.51 log CFU/mL	1329:1349	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	6	29	theme	log	1340:1342	arg1	stable					1321:1326	stable	1321:1326	stable	1321:1326	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	6	30	theme	complexes	1172:1180	arg1	microencapsulation					1182:1199	the casein/GA complexes microencapsulation	1158:1199	the casein/GA complexes microencapsulation	1158:1199	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	1	31	theme	use	221:223	arg1	histories					203:211	long histories	198:211	long histories of safe use	198:223	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	0	32	theme	Arabic/casein	19:31	arg1	microencapsulation					33:50	gum Arabic/casein microencapsulation	15:50	gum Arabic/casein microencapsulation	15:50	Enhancement of gum Arabic/casein microencapsulation on the survival of Lactiplantibacillus plantarum in the stimulated gastrointestinal conditions.					
37394217	4	33	theme	Confocal	917:924	arg1	Microscope					941:950	Confocal Laser Scanning Microscope	917:950	Confocal Laser Scanning Microscope	917:950	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	4	33	theme	Confocal	917:924	arg1	CLSM					911:914	CLSM	911:914	CLSM (Confocal Laser Scanning Microscope)	911:951	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	4	34	theme	GA	793:794	arg1	concentration					796:808	the GA concentration	789:808	the GA concentration	789:808	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	0	35	theme	gum	15:17	arg1	microencapsulation					33:50	gum Arabic/casein microencapsulation	15:50	gum Arabic/casein microencapsulation	15:50	Enhancement of gum Arabic/casein microencapsulation on the survival of Lactiplantibacillus plantarum in the stimulated gastrointestinal conditions.					
37394217	6	36	theme	viable	1211:1216	arg1	count					1218:1222	a higher viable count	1202:1222	a higher viable count	1202:1222	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	3	37	theme	Arabic	529:534	arg1	complexes					537:545	casein/GA (Gum Arabic) complexes	514:545	casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation	514:598	This study investigated the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation, and the stability of the strains during gastrointestinal environment were also determined.					
37394217	1	38	theme	Lactobacillus	228:240	arg1	strains					242:248	Lactobacillus strains	228:248	Lactobacillus strains	228:248	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	4	39	theme	particle	720:727	arg1	size					729:732	the particle size	716:732	the particle size of the emulsion	716:748	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	4	40	theme	Scanning	932:939	arg1	Microscope					941:950	Confocal Laser Scanning Microscope	917:950	Confocal Laser Scanning Microscope	917:950	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	4	40	theme	Scanning	932:939	arg1	CLSM					911:914	CLSM	911:914	CLSM (Confocal Laser Scanning Microscope)	911:951	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	3	41	theme	strains	626:632	arg1	stability					609:617	the stability	605:617	the stability of the strains during gastrointestinal environment	605:668	This study investigated the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation, and the stability of the strains during gastrointestinal environment were also determined.					
37394217	7	42	theme	oral	1508:1511	arg1	strategy					1522:1529	the oral delivery strategy	1504:1529	the oral delivery strategy	1504:1529	The results of study will help to design lactic acid bacteria encapsulation systems based on the GIT environment for the oral delivery strategy.					
37394217	7	43	theme	lactic	1428:1433	arg1	bacteria					1440:1447	lactic acid bacteria	1428:1447	lactic acid bacteria	1428:1447	The results of study will help to design lactic acid bacteria encapsulation systems based on the GIT environment for the oral delivery strategy.					
37394217	0	44	theme	microencapsulation	33:50	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of gum Arabic/casein microencapsulation on the survival of Lactiplantibacillus plantarum in the stimulated gastrointestinal conditions.	0:146	Enhancement of gum Arabic/casein microencapsulation on the survival of Lactiplantibacillus plantarum in the stimulated gastrointestinal conditions.					
37394217	4	45	theme	Laser	926:930	arg1	Microscope					941:950	Confocal Laser Scanning Microscope	917:950	Confocal Laser Scanning Microscope	917:950	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	4	45	theme	Laser	926:930	arg1	CLSM					911:914	CLSM	911:914	CLSM (Confocal Laser Scanning Microscope)	911:951	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	7	46	theme	delivery	1513:1520	arg1	strategy					1522:1529	the oral delivery strategy	1504:1529	the oral delivery strategy	1504:1529	The results of study will help to design lactic acid bacteria encapsulation systems based on the GIT environment for the oral delivery strategy.					
37394217	4	47	dep	2	830:830	arg1	to					827:828	to	827:828	to	827:828	The results showed that the particle size of the emulsion decreased from 9.72 μm to 5.48 μm when the GA concentration increased from 0 to 2 (w/v), and the emulsion particles were found to be more uniform as observed by CLSM (Confocal Laser Scanning Microscope).					
37394217	6	48	theme	storage	1370:1376	arg1	35 days					1359:1365	35 days	1359:1365	35 days of storage at 4 °C	1359:1384	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	3	49	theme	casein/GA	514:522	arg1	complexes					537:545	casein/GA (Gum Arabic) complexes	514:545	casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation	514:598	This study investigated the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation, and the stability of the strains during gastrointestinal environment were also determined.					
37394217	5	50	contain	has	1049:1051	arg1	surface					958:964	The surface	954:964	The surface of this microencapsulated casein/GA composite	954:1010	The surface of this microencapsulated casein/GA composite forms smooth, dense agglomerates and has high viscoelasticity, which effectively improved casein's emulsifying activity (8.66 ± 0.17 m2/g).					
37394217	5	50	contain	has	1049:1051	arg2	viscoelasticity					1058:1072	high viscoelasticity	1053:1072	high viscoelasticity	1053:1072	The surface of this microencapsulated casein/GA composite forms smooth, dense agglomerates and has high viscoelasticity, which effectively improved casein's emulsifying activity (8.66 ± 0.17 m2/g).					
37394217	1	51	contain	have	250:253	arg1	strains					242:248	Lactobacillus strains	228:248	Lactobacillus strains	228:248	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	1	51	contain	have	250:253	arg2	functions					274:282	many physiological functions	255:282	many physiological functions	255:282	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	6	52	theme	gastrointestinal	1243:1258	arg1	digestion					1260:1268	gastrointestinal digestion	1243:1268	gastrointestinal digestion	1243:1268	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	1	53	theme	Probiotic	148:156	arg1	products					158:165	Probiotic products	148:165	Probiotic products that contain lactobacilli	148:191	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	7	54	dep	systems	1463:1469	arg1	strategy					1522:1529	the oral delivery strategy	1504:1529	the oral delivery strategy	1504:1529	The results of study will help to design lactic acid bacteria encapsulation systems based on the GIT environment for the oral delivery strategy.					
37394217	5	55	theme	high	1053:1056	arg1	viscoelasticity					1058:1072	high viscoelasticity	1053:1072	high viscoelasticity	1053:1072	The surface of this microencapsulated casein/GA composite forms smooth, dense agglomerates and has high viscoelasticity, which effectively improved casein's emulsifying activity (8.66 ± 0.17 m2/g).					
37394217	5	56	theme	casein/GA	992:1000	arg1	composite					1002:1010	this microencapsulated casein/GA composite	969:1010	this microencapsulated casein/GA composite	969:1010	The surface of this microencapsulated casein/GA composite forms smooth, dense agglomerates and has high viscoelasticity, which effectively improved casein's emulsifying activity (8.66 ± 0.17 m2/g).					
37394217	6	57	theme	casein/GA	1162:1170	arg1	complexes					1172:1180	casein/GA complexes	1162:1180	the casein/GA complexes microencapsulation	1158:1199	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	1	58	theme	many	255:258	arg1	functions					274:282	many physiological functions	255:282	many physiological functions	255:282	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	5	59	theme	microencapsulated	974:990	arg1	composite					1002:1010	this microencapsulated casein/GA composite	969:1010	this microencapsulated casein/GA composite	969:1010	The surface of this microencapsulated casein/GA composite forms smooth, dense agglomerates and has high viscoelasticity, which effectively improved casein's emulsifying activity (8.66 ± 0.17 m2/g).					
37394217	5	60	theme	composite	1002:1010	arg1	surface					958:964	The surface	954:964	The surface of this microencapsulated casein/GA composite	954:1010	The surface of this microencapsulated casein/GA composite forms smooth, dense agglomerates and has high viscoelasticity, which effectively improved casein's emulsifying activity (8.66 ± 0.17 m2/g).					
37394217	2	61	theme	probiotics	347:356	arg1	viability					334:342	the viability	330:342	the viability of probiotics	330:356	However, the viability of probiotics can be affected by food processing and the adverse environment.					
37394217	1	62	theme	physiological	260:272	arg1	functions					274:282	many physiological functions	255:282	many physiological functions	255:282	Probiotic products that contain lactobacilli have long histories of safe use as Lactobacillus strains have many physiological functions in the gastrointestinal tract (GIT).					
37394217	7	63	theme	encapsulation	1449:1461	arg1	systems					1463:1469	encapsulation systems	1449:1469	encapsulation systems based on the GIT environment	1449:1498	The results of study will help to design lactic acid bacteria encapsulation systems based on the GIT environment for the oral delivery strategy.					
37394217	3	64	theme	gastrointestinal	641:656	arg1	environment					658:668	gastrointestinal environment	641:668	gastrointestinal environment	641:668	This study investigated the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation, and the stability of the strains during gastrointestinal environment were also determined.					
37394217	6	65	from	4 °C	1381:1384	arg1	storage					1370:1376	storage	1370:1376	storage at 4 °C	1370:1384	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	6	65	from	4 °C	1381:1384	arg1	35 days					1359:1365	35 days	1359:1365	35 days of storage at 4 °C	1359:1384	After the casein/GA complexes microencapsulation, a higher viable count was detected after gastrointestinal digestion in vitro, and the activity of L. plantarum is more stable (about 7.51 log CFU/mL) during 35 days of storage at 4 °C.					
37394217	5	66	theme	smooth	1018:1023	arg1	agglomerates					1032:1043	smooth, dense agglomerates	1018:1043	smooth, dense agglomerates	1018:1043	The surface of this microencapsulated casein/GA composite forms smooth, dense agglomerates and has high viscoelasticity, which effectively improved casein's emulsifying activity (8.66 ± 0.17 m2/g).					
37394217	3	67	theme	complexes	537:545	arg1	coagulation					499:509	coagulation	499:509	coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation	499:598	This study investigated the O/W (Oil-in-water emulsions) emulsions formed by coagulation of casein/GA (Gum Arabic) complexes for Lactiplantibacillus plantarum microencapsulation, and the stability of the strains during gastrointestinal environment were also determined.					
35579967	4	0	theme	mechanisms	843:852	arg1	modulation					823:832	modulation	823:832	modulation of these mechanisms by given precursor solution	823:880	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	4	0	theme	mechanisms	843:852	arg1	mechanism					780:788	the ice growth mechanism	765:788	the ice growth mechanism determined by the SFD system	765:817	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	11	1	from	use	2032:2034	arg1	delivery					2054:2061	pulmonary drug delivery	2039:2061	pulmonary drug delivery	2039:2061	The SFD dextran particles herein described could find use in pulmonary drug delivery due to their high porosity and biocompatibility of the matrix material.					
35579967	10	2	theme	significant	1925:1935	arg1	factor					1937:1942	the most significant factor	1916:1942	the most significant factor in affecting the pore dimensions	1916:1975	Analysis of variance and post hoc tests indicate that dextran concentration is the most significant factor in affecting the pore dimensions.					
35579967	10	2	theme	significant	1925:1935	arg1	concentration					1899:1911	dextran concentration	1891:1911	dextran concentration	1891:1911	Analysis of variance and post hoc tests indicate that dextran concentration is the most significant factor in affecting the pore dimensions.					
35579967	1	3	theme	ice	253:255	arg1	nucleation					257:266	ice nucleation	253:266	ice nucleation	253:266	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	7	4	theme	surface	1455:1461	arg1	area					1463:1466	the overall dendrite surface area	1434:1466	the overall dendrite surface area scaling approximately linearly with the particle diameter	1434:1524	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	4	5	theme	given	857:861	arg1	solution					873:880	given precursor solution	857:880	given precursor solution	857:880	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	6	6	theme	Image	1178:1182	arg1	analysis					1184:1191	Image analysis	1178:1191	Image analysis	1178:1191	Image analysis suggests that the pore volume decreases with dextran solid content.					
35579967	5	7	with	regime	1136:1141	arg1	density					1169:1175	high nucleation site density	1148:1175	high nucleation site density	1148:1175	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	3	8	theme	varying	532:538	arg1	weights					550:556	varying molecular weights	532:556	varying molecular weights (40-200 kDa)	532:569	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	3	8	theme	varying	532:538	arg1	kDa					566:568	40-200 kDa	559:568	40-200 kDa	559:568	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	5	9	from	variation	993:1001	arg1	diameter					1025:1032	diameter	1025:1032	diameter	1025:1032	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	7	10	theme	overall	1438:1444	arg1	area					1463:1466	the overall dendrite surface area	1434:1466	the overall dendrite surface area scaling approximately linearly with the particle diameter	1434:1524	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	7	11	theme	slower	1401:1406	arg1	regime					1415:1420	a slower growth regime	1399:1420	a slower growth regime	1399:1420	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	7	12	with	pores	1271:1275	arg1	region					1330:1335	often a central cellular region	1305:1335	often a central cellular region	1305:1335	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	4	13	theme	precursor	863:871	arg1	solution					873:880	given precursor solution	857:880	given precursor solution	857:880	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	4	14	theme	Coexisting	647:656	arg1	morphologies					658:669	Coexisting morphologies	647:669	Coexisting morphologies composed of cellular and dendritic motifs	647:711	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	5	15	with	Particles	938:946	arg1	pores					979:983	low-aspect ratio cellular pores	953:983	low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter	953:1103	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	6	16	theme	dextran	1238:1244	arg1	content					1252:1258	dextran solid content	1238:1258	dextran solid content	1238:1258	Image analysis suggests that the pore volume decreases with dextran solid content.					
35579967	6	17	theme	pore	1211:1214	arg1	volume					1216:1221	the pore volume	1207:1221	the pore volume	1207:1221	Image analysis suggests that the pore volume decreases with dextran solid content.					
35579967	3	18	theme	weights	550:556	arg1	solutions					519:527	aqueous dextran solutions	503:527	aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%)	503:596	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	1	19	theme	porous	192:197	arg1	particles					199:207	highly porous particles	185:207	highly porous particles	185:207	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	9	20	theme	cellular	1709:1716	arg1	pores					1718:1722	highly elongated cellular pores	1692:1722	highly elongated cellular pores without lamellar formation	1692:1749	Particles with highly elongated cellular pores without lamellar formation show intermediate pore dimensions between the above two limiting morphological types.					
35579967	5	21	dep	pores	979:983	arg1	constant					1073:1080	constant	1073:1080	constant	1073:1080	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	5	21	dep	pores	979:983	arg1	showing					985:991	showing	985:991	showing variation of around 0.5-1 μm in diameter with precursor composition	985:1059	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	4	22	from	changes	904:910	arg1	dimensions					926:935	their pore dimensions	915:935	their pore dimensions	915:935	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	3	23	theme	concentrations	575:588	arg1	solutions					519:527	aqueous dextran solutions	503:527	aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%)	503:596	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	4	24	theme	dendritic	696:704	arg1	motifs					706:711	cellular and dendritic motifs	683:711	cellular and dendritic motifs	683:711	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	11	25	theme	material	2125:2132	arg1	porosity					2081:2088	their high porosity	2070:2088	their high porosity	2070:2088	The SFD dextran particles herein described could find use in pulmonary drug delivery due to their high porosity and biocompatibility of the matrix material.					
35579967	11	25	theme	material	2125:2132	arg1	biocompatibility					2094:2109	biocompatibility	2094:2109	biocompatibility	2094:2109	The SFD dextran particles herein described could find use in pulmonary drug delivery due to their high porosity and biocompatibility of the matrix material.					
35579967	5	26	theme	precursor	1039:1047	arg1	composition					1049:1059	precursor composition	1039:1059	precursor composition	1039:1059	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	9	27	theme	intermediate	1756:1767	arg1	dimensions					1774:1783	intermediate pore dimensions	1756:1783	intermediate pore dimensions between the above two limiting morphological types	1756:1834	Particles with highly elongated cellular pores without lamellar formation show intermediate pore dimensions between the above two limiting morphological types.					
35579967	7	28	theme	cellular	1321:1328	arg1	region					1330:1335	often a central cellular region	1305:1335	often a central cellular region	1305:1335	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	11	29	theme	matrix	2118:2123	arg1	material					2125:2132	the matrix material	2114:2132	the matrix material	2114:2132	The SFD dextran particles herein described could find use in pulmonary drug delivery due to their high porosity and biocompatibility of the matrix material.					
35579967	4	30	theme	cellular	683:690	arg1	motifs					706:711	cellular and dendritic motifs	683:711	cellular and dendritic motifs	683:711	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	5	31	theme	rapid	1123:1127	arg1	regime					1136:1141	a rapid growth regime	1121:1141	a rapid growth regime with high nucleation site density	1121:1175	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	1	32	theme	templating	151:160	arg1	method					162:167	an ice templating method	144:167	an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth	144:277	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	1	32	theme	templating	151:160	arg1	drying					128:133	Spray freeze drying	115:133	Spray freeze drying (SFD)	115:139	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	5	33	theme	particle	1087:1094	arg1	diameter					1096:1103	particle diameter	1087:1103	particle diameter	1087:1103	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	2	34	theme	SFD	280:282	arg1	particles					284:292	SFD particles	280:292	SFD particles	280:292	SFD particles have been advanced as drug carrier systems, but the quantitative description of the morphology formation in the SFD process is still challenging.					
35579967	7	35	dep	pores	1271:1275	arg1	μm					1284:1285	≈2-20 μm	1278:1285	≈2-20 μm in diameter	1278:1297	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	3	36	theme	pore	450:453	arg1	dimensions					461:470	the pore space dimensions	446:470	the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%)	446:596	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	2	37	theme	formation	389:397	arg1	description					359:369	the quantitative description	342:369	the quantitative description of the morphology formation in the SFD process	342:416	SFD particles have been advanced as drug carrier systems, but the quantitative description of the morphology formation in the SFD process is still challenging.					
35579967	5	38	theme	cellular	970:977	arg1	pores					979:983	low-aspect ratio cellular pores	953:983	low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter	953:1103	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	7	39	theme	central	1313:1319	arg1	region					1330:1335	often a central cellular region	1305:1335	often a central cellular region	1305:1335	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	9	40	theme	morphological	1816:1828	arg1	types					1830:1834	the above two limiting morphological types	1793:1834	the above two limiting morphological types	1793:1834	Particles with highly elongated cellular pores without lamellar formation show intermediate pore dimensions between the above two limiting morphological types.					
35579967	5	41	theme	site	1164:1167	arg1	density					1169:1175	high nucleation site density	1148:1175	high nucleation site density	1148:1175	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	11	42	theme	pulmonary	2039:2047	arg1	delivery					2054:2061	pulmonary drug delivery	2039:2061	pulmonary drug delivery	2039:2061	The SFD dextran particles herein described could find use in pulmonary drug delivery due to their high porosity and biocompatibility of the matrix material.					
35579967	8	43	theme	dendrite	1531:1538	arg1	spacing					1549:1555	The dendrite lamellar spacing	1527:1555	The dendrite lamellar spacing	1527:1555	The dendrite lamellar spacing depends on the concentration according to an inverse power law but is not significantly influenced by molecular weight.					
35579967	0	44	theme	Pore	15:18	arg1	Architecture					20:31	the Pore Architecture	11:31	the Pore Architecture of Ice-Templated Dextran Microparticles Using Molecular Weight and Concentration	11:112	Modulating the Pore Architecture of Ice-Templated Dextran Microparticles Using Molecular Weight and Concentration.					
35579967	1	45	theme	freeze	121:126	arg1	SFD					136:138	SFD	136:138	SFD	136:138	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	1	45	theme	freeze	121:126	arg1	drying					128:133	Spray freeze drying	115:133	Spray freeze drying (SFD)	115:139	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	1	45	theme	freeze	121:126	arg1	method					162:167	an ice templating method	144:167	an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth	144:277	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	3	46	theme	scanning	617:624	arg1	microscopy					635:644	scanning electron microscopy	617:644	scanning electron microscopy	617:644	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	7	47	theme	particle	1508:1515	arg1	diameter					1517:1524	the particle diameter	1504:1524	the particle diameter	1504:1524	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	7	48	from	μm	1284:1285	arg1	diameter					1290:1297	diameter	1290:1297	diameter	1290:1297	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	4	49	theme	SFD	808:810	arg1	system					812:817	the SFD system	804:817	the SFD system	804:817	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	9	50	with	Particles	1677:1685	arg1	pores					1718:1722	highly elongated cellular pores	1692:1722	highly elongated cellular pores without lamellar formation	1692:1749	Particles with highly elongated cellular pores without lamellar formation show intermediate pore dimensions between the above two limiting morphological types.					
35579967	0	51	theme	Dextran	50:56	arg1	Microparticles					58:71	Ice-Templated Dextran Microparticles	36:71	Ice-Templated Dextran Microparticles Using Molecular Weight and Concentration	36:112	Modulating the Pore Architecture of Ice-Templated Dextran Microparticles Using Molecular Weight and Concentration.					
35579967	5	52	theme	high	1148:1151	arg1	density					1169:1175	high nucleation site density	1148:1175	high nucleation site density	1148:1175	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	4	53	theme	ice	769:771	arg1	mechanism					780:788	the ice growth mechanism	765:788	the ice growth mechanism determined by the SFD system	765:817	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	3	54	theme	space	455:459	arg1	dimensions					461:470	the pore space dimensions	446:470	the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%)	446:596	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	1	55	theme	complex	214:220	arg1	architectures					227:239	complex pore architectures	214:239	complex pore architectures governed by ice nucleation and growth	214:277	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	10	56	theme	dextran	1891:1897	arg1	factor					1937:1942	the most significant factor	1916:1942	the most significant factor in affecting the pore dimensions	1916:1975	Analysis of variance and post hoc tests indicate that dextran concentration is the most significant factor in affecting the pore dimensions.					
35579967	10	56	theme	dextran	1891:1897	arg1	concentration					1899:1911	dextran concentration	1891:1911	dextran concentration	1891:1911	Analysis of variance and post hoc tests indicate that dextran concentration is the most significant factor in affecting the pore dimensions.					
35579967	8	57	theme	inverse	1602:1608	arg1	law					1616:1618	an inverse power law	1599:1618	an inverse power law	1599:1618	The dendrite lamellar spacing depends on the concentration according to an inverse power law but is not significantly influenced by molecular weight.					
35579967	3	58	theme	particles	479:487	arg1	dimensions					461:470	the pore space dimensions	446:470	the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%)	446:596	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	7	59	theme	dendrite	1446:1453	arg1	area					1463:1466	the overall dendrite surface area	1434:1466	the overall dendrite surface area scaling approximately linearly with the particle diameter	1434:1524	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	10	60	theme	pore	1961:1964	arg1	dimensions					1966:1975	the pore dimensions	1957:1975	the pore dimensions	1957:1975	Analysis of variance and post hoc tests indicate that dextran concentration is the most significant factor in affecting the pore dimensions.					
35579967	3	61	theme	dextran	511:517	arg1	solutions					519:527	aqueous dextran solutions	503:527	aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%)	503:596	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	4	62	dep	mechanism	780:788	arg1	properties					882:891	properties	882:891	properties	882:891	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	7	63	theme	growth	1408:1413	arg1	regime					1415:1420	a slower growth regime	1399:1420	a slower growth regime	1399:1420	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	6	64	theme	solid	1246:1250	arg1	content					1252:1258	dextran solid content	1238:1258	dextran solid content	1238:1258	Image analysis suggests that the pore volume decreases with dextran solid content.					
35579967	2	65	from	description	359:369	arg1	process					410:416	the SFD process	402:416	the SFD process	402:416	SFD particles have been advanced as drug carrier systems, but the quantitative description of the morphology formation in the SFD process is still challenging.					
35579967	3	66	theme	molecular	540:548	arg1	weights					550:556	varying molecular weights	532:556	varying molecular weights (40-200 kDa)	532:569	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	3	66	theme	molecular	540:548	arg1	kDa					566:568	40-200 kDa	559:568	40-200 kDa	559:568	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	9	67	theme	elongated	1699:1707	arg1	pores					1718:1722	highly elongated cellular pores	1692:1722	highly elongated cellular pores without lamellar formation	1692:1749	Particles with highly elongated cellular pores without lamellar formation show intermediate pore dimensions between the above two limiting morphological types.					
35579967	11	68	theme	SFD	1982:1984	arg1	particles					1994:2002	The SFD dextran particles	1978:2002	The SFD dextran particles herein described	1978:2019	The SFD dextran particles herein described could find use in pulmonary drug delivery due to their high porosity and biocompatibility of the matrix material.					
35579967	11	69	theme	high	2076:2079	arg1	porosity					2081:2088	their high porosity	2070:2088	their high porosity	2070:2088	The SFD dextran particles herein described could find use in pulmonary drug delivery due to their high porosity and biocompatibility of the matrix material.					
35579967	9	70	theme	lamellar	1732:1739	arg1	formation					1741:1749	lamellar formation	1732:1749	lamellar formation	1732:1749	Particles with highly elongated cellular pores without lamellar formation show intermediate pore dimensions between the above two limiting morphological types.					
35579967	7	71	theme	surface	1357:1363	arg1	nucleation					1365:1374	surface nucleation	1357:1374	surface nucleation	1357:1374	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	10	72	theme	post	1862:1865	arg1	hoc					1867:1869	post hoc	1862:1869	post hoc	1862:1869	Analysis of variance and post hoc tests indicate that dextran concentration is the most significant factor in affecting the pore dimensions.					
35579967	11	73	theme	drug	2049:2052	arg1	delivery					2054:2061	pulmonary drug delivery	2039:2061	pulmonary drug delivery	2039:2061	The SFD dextran particles herein described could find use in pulmonary drug delivery due to their high porosity and biocompatibility of the matrix material.					
35579967	2	74	theme	carrier	321:327	arg1	systems					329:335	drug carrier systems	316:335	drug carrier systems	316:335	SFD particles have been advanced as drug carrier systems, but the quantitative description of the morphology formation in the SFD process is still challenging.					
35579967	0	75	theme	Molecular	79:87	arg1	Weight					89:94	Molecular Weight	79:94	Molecular Weight	79:94	Modulating the Pore Architecture of Ice-Templated Dextran Microparticles Using Molecular Weight and Concentration.					
35579967	10	76	theme	variance	1849:1856	arg1	hoc					1867:1869	post hoc	1862:1869	post hoc	1862:1869	Analysis of variance and post hoc tests indicate that dextran concentration is the most significant factor in affecting the pore dimensions.					
35579967	10	76	theme	variance	1849:1856	arg1	Analysis					1837:1844	Analysis	1837:1844	Analysis of variance	1837:1856	Analysis of variance and post hoc tests indicate that dextran concentration is the most significant factor in affecting the pore dimensions.					
35579967	9	77	theme	pore	1769:1772	arg1	dimensions					1774:1783	intermediate pore dimensions	1756:1783	intermediate pore dimensions between the above two limiting morphological types	1756:1834	Particles with highly elongated cellular pores without lamellar formation show intermediate pore dimensions between the above two limiting morphological types.					
35579967	9	78	theme	above	1797:1801	arg1	types					1830:1834	the above two limiting morphological types	1793:1834	the above two limiting morphological types	1793:1834	Particles with highly elongated cellular pores without lamellar formation show intermediate pore dimensions between the above two limiting morphological types.					
35579967	1	79	theme	ice	147:149	arg1	method					162:167	an ice templating method	144:167	an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth	144:277	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	1	79	theme	ice	147:149	arg1	drying					128:133	Spray freeze drying	115:133	Spray freeze drying (SFD)	115:139	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	5	80	theme	growth	1129:1134	arg1	regime					1136:1141	a rapid growth regime	1121:1141	a rapid growth regime with high nucleation site density	1121:1175	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	4	81	from	variations	751:760	arg1	modulation					823:832	modulation	823:832	modulation of these mechanisms by given precursor solution	823:880	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	4	81	from	variations	751:760	arg1	mechanism					780:788	the ice growth mechanism	765:788	the ice growth mechanism determined by the SFD system	765:817	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	4	82	theme	pore	921:924	arg1	dimensions					926:935	their pore dimensions	915:935	their pore dimensions	915:935	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	5	83	theme	low-aspect	953:962	arg1	pores					979:983	low-aspect ratio cellular pores	953:983	low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter	953:1103	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	5	84	theme	ratio	964:968	arg1	pores					979:983	low-aspect ratio cellular pores	953:983	low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter	953:1103	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	9	85	theme	limiting	1807:1814	arg1	types					1830:1834	the above two limiting morphological types	1793:1834	the above two limiting morphological types	1793:1834	Particles with highly elongated cellular pores without lamellar formation show intermediate pore dimensions between the above two limiting morphological types.					
35579967	2	86	theme	morphology	378:387	arg1	formation					389:397	the morphology formation	374:397	the morphology formation	374:397	SFD particles have been advanced as drug carrier systems, but the quantitative description of the morphology formation in the SFD process is still challenging.					
35579967	8	87	theme	lamellar	1540:1547	arg1	spacing					1549:1555	The dendrite lamellar spacing	1527:1555	The dendrite lamellar spacing	1527:1555	The dendrite lamellar spacing depends on the concentration according to an inverse power law but is not significantly influenced by molecular weight.					
35579967	5	88	with	constant	1073:1080	arg1	diameter					1096:1103	particle diameter	1087:1103	particle diameter	1087:1103	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	1	89	theme	Spray	115:119	arg1	SFD					136:138	SFD	136:138	SFD	136:138	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	1	89	theme	Spray	115:119	arg1	drying					128:133	Spray freeze drying	115:133	Spray freeze drying (SFD)	115:139	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	1	89	theme	Spray	115:119	arg1	method					162:167	an ice templating method	144:167	an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth	144:277	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	2	90	theme	SFD	406:408	arg1	process					410:416	the SFD process	402:416	the SFD process	402:416	SFD particles have been advanced as drug carrier systems, but the quantitative description of the morphology formation in the SFD process is still challenging.					
35579967	0	91	theme	Ice-Templated	36:48	arg1	Microparticles					58:71	Ice-Templated Dextran Microparticles	36:71	Ice-Templated Dextran Microparticles Using Molecular Weight and Concentration	36:112	Modulating the Pore Architecture of Ice-Templated Dextran Microparticles Using Molecular Weight and Concentration.					
35579967	10	92	dep	Analysis	1837:1844	arg1	tests					1871:1875	tests	1871:1875	tests	1871:1875	Analysis of variance and post hoc tests indicate that dextran concentration is the most significant factor in affecting the pore dimensions.					
35579967	0	93	theme	Microparticles	58:71	arg1	Architecture					20:31	the Pore Architecture	11:31	the Pore Architecture of Ice-Templated Dextran Microparticles Using Molecular Weight and Concentration	11:112	Modulating the Pore Architecture of Ice-Templated Dextran Microparticles Using Molecular Weight and Concentration.					
35579967	5	94	theme	μm	1019:1020	arg1	variation					993:1001	variation	993:1001	variation of around 0.5-1 μm in diameter	993:1032	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	2	95	theme	quantitative	346:357	arg1	description					359:369	the quantitative description	342:369	the quantitative description of the morphology formation in the SFD process	342:416	SFD particles have been advanced as drug carrier systems, but the quantitative description of the morphology formation in the SFD process is still challenging.					
35579967	3	96	theme	SFD	475:477	arg1	particles					479:487	SFD particles	475:487	SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%)	475:596	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	5	97	theme	nucleation	1153:1162	arg1	density					1169:1175	high nucleation site density	1148:1175	high nucleation site density	1148:1175	Particles with low-aspect ratio cellular pores showing variation of around 0.5-1 μm in diameter with precursor composition but roughly constant with particle diameter are ascribed to a rapid growth regime with high nucleation site density.					
35579967	11	98	theme	dextran	1986:1992	arg1	particles					1994:2002	The SFD dextran particles	1978:2002	The SFD dextran particles herein described	1978:2019	The SFD dextran particles herein described could find use in pulmonary drug delivery due to their high porosity and biocompatibility of the matrix material.					
35579967	7	99	theme	Dendritic	1261:1269	arg1	pores					1271:1275	Dendritic pores	1261:1275	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region	1261:1335	Dendritic pores (≈2-20 μm in diameter) with often a central cellular region are identified with surface nucleation and growth followed by a slower growth regime, leading to the overall dendrite surface area scaling approximately linearly with the particle diameter.					
35579967	3	100	theme	electron	626:633	arg1	microscopy					635:644	scanning electron microscopy	617:644	scanning electron microscopy	617:644	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
35579967	2	101	theme	drug	316:319	arg1	systems					329:335	drug carrier systems	316:335	drug carrier systems	316:335	SFD particles have been advanced as drug carrier systems, but the quantitative description of the morphology formation in the SFD process is still challenging.					
35579967	8	102	theme	molecular	1659:1667	arg1	weight					1669:1674	molecular weight	1659:1674	molecular weight	1659:1674	The dendrite lamellar spacing depends on the concentration according to an inverse power law but is not significantly influenced by molecular weight.					
35579967	1	103	theme	pore	222:225	arg1	architectures					227:239	complex pore architectures	214:239	complex pore architectures governed by ice nucleation and growth	214:277	Spray freeze drying (SFD) is an ice templating method used to produce highly porous particles with complex pore architectures governed by ice nucleation and growth.					
35579967	8	104	theme	power	1610:1614	arg1	law					1616:1618	an inverse power law	1599:1618	an inverse power law	1599:1618	The dendrite lamellar spacing depends on the concentration according to an inverse power law but is not significantly influenced by molecular weight.					
35579967	4	105	theme	growth	773:778	arg1	mechanism					780:788	the ice growth mechanism	765:788	the ice growth mechanism determined by the SFD system	765:817	Coexisting morphologies composed of cellular and dendritic motifs are obtained, which are attributed to variations in the ice growth mechanism determined by the SFD system and modulation of these mechanisms by given precursor solution properties leading to changes in their pore dimensions.					
35579967	3	106	theme	aqueous	503:509	arg1	solutions					519:527	aqueous dextran solutions	503:527	aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%)	503:596	Here, the pore space dimensions of SFD particles prepared from aqueous dextran solutions of varying molecular weights (40-200 kDa) and concentrations (5-20%) are analyzed using scanning electron microscopy.					
36277712	0	0	with	patients	82:89	arg1	herniation					110:119	cervical disc herniation	96:119	cervical disc herniation	96:119	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.					
36277712	4	1	theme	mentioned	842:850	arg1	purposes					861:868	the above mentioned research purposes	832:868	the above mentioned research purposes	832:868	Methods Our study used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes.					
36277712	12	2	dep	staining	2072:2079	arg1	staining					2086:2093	H&E staining	2082:2093	H&E staining	2082:2093	Moreover, histological staining (H&E staining and Safranin-O staining) showed the general structure of the NP and the distribution of macromolecules.					
36277712	12	2	dep	staining	2072:2079	arg1	staining					2110:2117	Safranin-O staining	2099:2117	Safranin-O staining	2099:2117	Moreover, histological staining (H&E staining and Safranin-O staining) showed the general structure of the NP and the distribution of macromolecules.					
36277712	3	3	theme	cervical	505:512	arg1	NP					547:548	NP	547:548	NP	547:548	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	3	theme	cervical	505:512	arg1	pulposus					537:544	cervical disc herniated nucleus pulposus	505:544	cervical disc herniated nucleus pulposus (NP)	505:549	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	1	4	theme	intervertebral	244:257	arg1	IVDD					278:281	IVDD	278:281	IVDD	278:281	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	1	4	theme	intervertebral	244:257	arg1	degeneration					264:275	intervertebral disc degeneration	244:275	intervertebral disc degeneration (IVDD)	244:282	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	1	4	theme	intervertebral	244:257	arg1	cause					324:328	its primary cause	312:328	its primary cause	312:328	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	4	5	theme	disc	808:811	arg1	NP					813:814	in vitro intervertebral disc NP	784:814	in vitro intervertebral disc NP	784:814	Methods Our study used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes.					
36277712	6	6	theme	responsible	1010:1020	arg1	segment					1022:1028	the responsible segment	1006:1028	the responsible segment	1006:1028	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	3	7	theme	IVDD	671:674	arg1	mechanism					658:666	the mechanism	654:666	the mechanism of IVDD at the microscopic level	654:699	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	6	8	theme	NP	1210:1211	arg1	value					1201:1205	the T2 signal intensity value	1177:1205	the T2 signal intensity value of NP	1177:1211	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	3	9	theme	collagen	493:500	arg1	structures					462:471	structures	462:471	structures	462:471	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	9	theme	collagen	493:500	arg1	components					447:456	components	447:456	components	447:456	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	11	10	contain	have	1970:1973	arg1	it					1963:1964	it	1963:1964	it	1963:1964	For the microstructural characterization of collagen, we found that it may have an essential role in the degenerative process of the intervertebral disc.					
36277712	11	10	contain	have	1970:1973	arg2	role					1988:1991	an essential role	1975:1991	an essential role	1975:1991	For the microstructural characterization of collagen, we found that it may have an essential role in the degenerative process of the intervertebral disc.					
36277712	3	11	theme	herniated	519:527	arg1	NP					547:548	NP	547:548	NP	547:548	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	11	theme	herniated	519:527	arg1	pulposus					537:544	cervical disc herniated nucleus pulposus	505:544	cervical disc herniated nucleus pulposus (NP)	505:549	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	4	12	dep	in	784:785	arg1	vitro					787:791	vitro	787:791	vitro	787:791	Methods Our study used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes.					
36277712	6	13	theme	intensity	1191:1199	arg1	value					1201:1205	the T2 signal intensity value	1177:1205	the T2 signal intensity value of NP	1177:1211	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	10	14	theme	intensity	1836:1844	arg1	value					1846:1850	the NP intensity value	1829:1850	the NP intensity value	1829:1850	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	13	15	theme	Raman	2343:2347	arg1	spectroscopy					2349:2360	Raman spectroscopy	2343:2360	Raman spectroscopy	2343:2360	Conclusion The present study demonstrated the possibility of characterizing the macromolecular substances inside the cervical disc NP tissue by Raman spectroscopy.					
36277712	10	16	theme	I	1692:1692	arg1	peaks					1685:1689	the two peaks	1677:1689	the two peaks (I 1,064/ I 1,004) in the NP	1677:1718	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	10	16	theme	I	1692:1692	arg1	I					1701:1701	I 1,064/ I 1,004	1692:1707	I 1,064/ I 1,004	1692:1707	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	8	17	theme	Raman	1388:1392	arg1	spectroscopy					1394:1405	Raman spectroscopy	1388:1405	Raman spectroscopy	1388:1405	Finally, the samples were scanned and analyzed by Raman spectroscopy.					
36277712	6	18	theme	T2	1181:1182	arg1	intensity					1191:1199	T2 signal intensity	1181:1199	the T2 signal intensity value of NP	1177:1211	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	10	19	theme	peaks	1685:1689	arg1	ratio					1668:1672	the ratio	1664:1672	the ratio of the two peaks (I 1,064/ I 1,004) in the NP	1664:1718	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	4	20	dep	Methods	702:708	arg1	used					720:723	used	720:723	used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes	720:868	Methods Our study used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes.					
36277712	10	21	theme	positive	1861:1868	arg1	P					1883:1883	P < 0.001	1883:1891	P < 0.001	1883:1891	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	10	21	theme	positive	1861:1868	arg1	correlation					1870:1880	a positive correlation	1859:1880	a positive correlation (P < 0.001)	1859:1892	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	13	22	theme	disc	2325:2328	arg1	tissue					2333:2338	the cervical disc NP tissue	2312:2338	the cervical disc NP tissue by Raman spectroscopy	2312:2360	Conclusion The present study demonstrated the possibility of characterizing the macromolecular substances inside the cervical disc NP tissue by Raman spectroscopy.					
36277712	3	23	theme	convenient	573:582	arg1	technique					598:606	a validated and convenient Raman spectra technique	557:606	a validated and convenient Raman spectra technique	557:606	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	9	24	theme	tissues	1433:1439	arg1	total					1418:1422	A total	1416:1422	A total of 28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH	1416:1527	Results A total of 28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH were included in this study.					
36277712	11	25	theme	disc	2043:2046	arg1	process					2013:2019	the degenerative process	1996:2019	the degenerative process of the intervertebral disc	1996:2046	For the microstructural characterization of collagen, we found that it may have an essential role in the degenerative process of the intervertebral disc.					
36277712	7	26	theme	histological	1276:1287	arg1	staining					1289:1296	histological staining	1276:1296	histological staining (H&E staining and Safranin-O staining)	1276:1335	In addition, the structure of the NP samples was evaluated by histological staining (H&E staining and Safranin-O staining).					
36277712	3	27	from	pulposus	537:544	arg1	structures					462:471	structures	462:471	structures	462:471	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	27	from	pulposus	537:544	arg1	components					447:456	components	447:456	components	447:456	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	0	28	theme	nucleus	62:68	arg1	pulposus					70:77	the nucleus pulposus	58:77	the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy	58:141	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.					
36277712	3	29	theme	validated	559:567	arg1	technique					598:606	a validated and convenient Raman spectra technique	557:606	a validated and convenient Raman spectra technique	557:606	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	1	30	theme	Cervical	152:159	arg1	CDH					178:180	CDH	178:180	CDH	178:180	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	1	30	theme	Cervical	152:159	arg1	herniation					166:175	Purpose Cervical disc herniation	144:175	Purpose Cervical disc herniation (CDH)	144:181	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	12	31	theme	Safranin-O	2099:2108	arg1	staining					2110:2117	Safranin-O staining	2099:2117	Safranin-O staining	2099:2117	Moreover, histological staining (H&E staining and Safranin-O staining) showed the general structure of the NP and the distribution of macromolecules.					
36277712	9	32	with	patients	1449:1456	arg1	CDH					1525:1527	CDH	1525:1527	CDH	1525:1527	Results A total of 28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH were included in this study.					
36277712	14	33	theme	mechanism	2595:2603	arg1	elucidation					2574:2584	the elucidation	2570:2584	the elucidation of CDH's mechanism	2570:2603	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	3	34	from	collagen	493:500	arg1	NP					547:548	NP	547:548	NP	547:548	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	34	from	collagen	493:500	arg1	pulposus					537:544	cervical disc herniated nucleus pulposus	505:544	cervical disc herniated nucleus pulposus (NP)	505:549	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	10	35	theme	<	1885:1885	arg1	P					1883:1883	P < 0.001	1883:1891	P < 0.001	1883:1891	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	10	35	theme	<	1885:1885	arg1	correlation					1870:1880	a positive correlation	1859:1880	a positive correlation (P < 0.001)	1859:1892	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	0	36	from	Identification	0:13	arg1	pulposus					70:77	the nucleus pulposus	58:77	the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy	58:141	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.					
36277712	5	37	theme	cervical	893:900	arg1	samples					910:916	cervical disc NP samples	893:916	cervical disc NP samples	893:916	Firstly, we collected cervical disc NP samples and imaging data by certain inclusion and exclusion criteria.					
36277712	3	38	from	level	695:699	arg1	mechanism					658:666	the mechanism	654:666	the mechanism of IVDD at the microscopic level	654:699	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	4	39	theme	burgeoning	727:736	arg1	technique					738:746	a burgeoning technique	725:746	a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP	725:814	Methods Our study used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes.					
36277712	2	40	theme	intervertebral	357:370	arg1	degeneration					377:388	intervertebral disc degeneration	357:388	intervertebral disc degeneration	357:388	However, the mechanism of intervertebral disc degeneration is still unclear.					
36277712	0	41	theme	disc	105:108	arg1	herniation					110:119	cervical disc herniation	96:119	cervical disc herniation	96:119	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.					
36277712	5	42	theme	certain	938:944	arg1	criteria					970:977	certain inclusion and exclusion criteria	938:977	certain inclusion and exclusion criteria	938:977	Firstly, we collected cervical disc NP samples and imaging data by certain inclusion and exclusion criteria.					
36277712	6	43	theme	segment	1022:1028	arg1	NP					1000:1001	the NP	996:1001	the NP of the responsible segment	996:1028	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	9	44	dep	patients	1449:1456	arg1	cases					1486:1490	cases	1486:1490	cases that involved two segments	1486:1517	Results A total of 28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH were included in this study.					
36277712	1	45	theme	disc	259:262	arg1	IVDD					278:281	IVDD	278:281	IVDD	278:281	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	1	45	theme	disc	259:262	arg1	degeneration					264:275	intervertebral disc degeneration	244:275	intervertebral disc degeneration (IVDD)	244:282	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	1	45	theme	disc	259:262	arg1	cause					324:328	its primary cause	312:328	its primary cause	312:328	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	5	46	theme	NP	907:908	arg1	samples					910:916	cervical disc NP samples	893:916	cervical disc NP samples	893:916	Firstly, we collected cervical disc NP samples and imaging data by certain inclusion and exclusion criteria.					
36277712	3	47	theme	study	423:427	arg1	aim					412:414	The aim	408:414	The aim of the study	408:427	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	0	48	theme	compositional	18:30	arg1	changes					47:53	compositional and structural changes	18:53	compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy	18:141	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.					
36277712	7	49	theme	NP	1248:1249	arg1	samples					1251:1257	the NP samples	1244:1257	the NP samples	1244:1257	In addition, the structure of the NP samples was evaluated by histological staining (H&E staining and Safranin-O staining).					
36277712	2	50	theme	degeneration	377:388	arg1	unclear					399:405	unclear	399:405	unclear	399:405	However, the mechanism of intervertebral disc degeneration is still unclear.					
36277712	2	50	theme	degeneration	377:388	arg1	mechanism					344:352	the mechanism	340:352	the mechanism of intervertebral disc degeneration	340:388	However, the mechanism of intervertebral disc degeneration is still unclear.					
36277712	0	51	theme	structural	36:45	arg1	changes					47:53	compositional and structural changes	18:53	compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy	18:141	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.					
36277712	3	52	theme	spectra	590:596	arg1	technique					598:606	a validated and convenient Raman spectra technique	557:606	a validated and convenient Raman spectra technique	557:606	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	10	53	dep	spectroscopy	1581:1592	arg1	scan					1594:1597	scan	1594:1597	scan	1594:1597	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	10	54	theme	relative	1604:1611	arg1	content					1613:1619	the relative content	1600:1619	the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP	1600:1718	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	6	55	theme	magnetic	1079:1086	arg1	MRI					1107:1109	MRI	1107:1109	MRI	1107:1109	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	6	55	theme	magnetic	1079:1086	arg1	imaging					1098:1104	the patient's preoperative cervical magnetic resonance imaging	1043:1104	the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images	1043:1129	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	1	56	theme	common	202:207	arg1	diseases					216:223	the most common spinal diseases	193:223	the most common spinal diseases in modern society	193:241	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	6	57	theme	preoperative	1057:1068	arg1	MRI					1107:1109	MRI	1107:1109	MRI	1107:1109	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	6	57	theme	preoperative	1057:1068	arg1	imaging					1098:1104	the patient's preoperative cervical magnetic resonance imaging	1043:1104	the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images	1043:1129	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	4	58	theme	spectroscopy	757:768	arg1	technique					738:746	a burgeoning technique	725:746	a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP	725:814	Methods Our study used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes.					
36277712	4	59	used	used	720:723	arg2	study					714:718	Our study	710:718	Our study	710:718	Methods Our study used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes.					
36277712	1	60	theme	diseases	216:223	arg1	diseases					216:223	the most common spinal diseases	193:223	the most common spinal diseases in modern society	193:241	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	1	60	theme	diseases	216:223	arg1	one					186:188	one	186:188	one	186:188	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	13	61	theme	NP	2330:2331	arg1	tissue					2333:2338	the cervical disc NP tissue	2312:2338	the cervical disc NP tissue by Raman spectroscopy	2312:2360	Conclusion The present study demonstrated the possibility of characterizing the macromolecular substances inside the cervical disc NP tissue by Raman spectroscopy.					
36277712	14	62	from	degree	2457:2462	arg1	structure					2493:2501	structure	2493:2501	structure	2493:2501	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	14	62	from	degree	2457:2462	arg1	content					2481:2487	content	2481:2487	content	2481:2487	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	9	63	from	patients	1449:1456	arg1	tissues					1433:1439	28 NP tissues	1427:1439	28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH	1427:1527	Results A total of 28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH were included in this study.					
36277712	9	63	from	patients	1449:1456	arg1	total					1418:1422	A total	1416:1422	A total of 28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH	1416:1527	Results A total of 28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH were included in this study.					
36277712	10	64	with	correlation	1752:1762	arg1	P					1786:1786	P < 0.001	1786:1794	P < 0.001	1786:1794	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	10	64	with	correlation	1752:1762	arg1	grade					1779:1783	Pfirrmann grade	1769:1783	Pfirrmann grade (P < 0.001)	1769:1795	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	10	65	theme	Pfirrmann	1769:1777	arg1	P					1786:1786	P < 0.001	1786:1794	P < 0.001	1786:1794	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	10	65	theme	Pfirrmann	1769:1777	arg1	grade					1779:1783	Pfirrmann grade	1769:1783	Pfirrmann grade (P < 0.001)	1769:1795	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	3	66	from	structures	462:471	arg1	NP					547:548	NP	547:548	NP	547:548	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	66	from	structures	462:471	arg1	pulposus					537:544	cervical disc herniated nucleus pulposus	505:544	cervical disc herniated nucleus pulposus (NP)	505:549	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	4	67	theme	above	836:840	arg1	purposes					861:868	the above mentioned research purposes	832:868	the above mentioned research purposes	832:868	Methods Our study used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes.					
36277712	12	68	theme	macromolecules	2183:2196	arg1	structure					2139:2147	the general structure	2127:2147	the general structure of the NP	2127:2157	Moreover, histological staining (H&E staining and Safranin-O staining) showed the general structure of the NP and the distribution of macromolecules.					
36277712	12	68	theme	macromolecules	2183:2196	arg1	distribution					2167:2178	the distribution	2163:2178	the distribution of macromolecules	2163:2196	Moreover, histological staining (H&E staining and Safranin-O staining) showed the general structure of the NP and the distribution of macromolecules.					
36277712	3	69	theme	microscopic	683:693	arg1	level					695:699	the microscopic level	679:699	the microscopic level	679:699	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	70	from	proteoglycan	476:487	arg1	NP					547:548	NP	547:548	NP	547:548	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	70	from	proteoglycan	476:487	arg1	pulposus					537:544	cervical disc herniated nucleus pulposus	505:544	cervical disc herniated nucleus pulposus (NP)	505:549	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	13	71	dep	Conclusion	2199:2208	arg1	demonstrated					2228:2239	demonstrated	2228:2239	demonstrated the possibility of characterizing the macromolecular substances inside the cervical disc NP tissue by Raman spectroscopy	2228:2360	Conclusion The present study demonstrated the possibility of characterizing the macromolecular substances inside the cervical disc NP tissue by Raman spectroscopy.					
36277712	7	72	dep	staining	1303:1310	arg1	staining					1327:1334	and Safranin-O staining	1312:1334	H&E staining and Safranin-O staining	1299:1334	In addition, the structure of the NP samples was evaluated by histological staining (H&E staining and Safranin-O staining).					
36277712	4	73	theme	intervertebral	793:806	arg1	NP					813:814	in vitro intervertebral disc NP	784:814	in vitro intervertebral disc NP	784:814	Methods Our study used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes.					
36277712	6	74	theme	T2-weighted	1112:1122	arg1	images					1124:1129	the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images	1043:1129	the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images	1043:1129	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	14	75	contain	have	2447:2450	arg2	degree					2457:2462	some degree	2452:2462	some degree of alteration in content and structure	2452:2501	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	14	75	contain	have	2447:2450	arg1	proteoglycans					2420:2432	proteoglycans	2420:2432	proteoglycans	2420:2432	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	14	75	contain	have	2447:2450	arg1	collagen					2438:2445	collagen	2438:2445	collagen	2438:2445	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	14	75	contain	have	2447:2450	arg1	substances					2401:2410	macromolecular substances	2386:2410	macromolecular substances such as proteoglycans and collagen	2386:2445	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	14	76	from	structure	2493:2501	arg1	degree					2457:2462	some degree	2452:2462	some degree of alteration in content and structure	2452:2501	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	10	77	theme	<	1788:1788	arg1	P					1786:1786	P < 0.001	1786:1794	P < 0.001	1786:1794	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	10	77	theme	<	1788:1788	arg1	grade					1779:1783	Pfirrmann grade	1769:1783	Pfirrmann grade (P < 0.001)	1769:1795	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	3	78	theme	nucleus	529:535	arg1	NP					547:548	NP	547:548	NP	547:548	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	78	theme	nucleus	529:535	arg1	pulposus					537:544	cervical disc herniated nucleus pulposus	505:544	cervical disc herniated nucleus pulposus (NP)	505:549	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	10	79	theme	collagen	1808:1815	arg1	content					1817:1823	the collagen content	1804:1823	the collagen content	1804:1823	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	0	80	theme	Raman	124:128	arg1	spectroscopy					130:141	Raman spectroscopy	124:141	Raman spectroscopy	124:141	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.					
36277712	3	81	from	components	447:456	arg1	NP					547:548	NP	547:548	NP	547:548	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	81	from	components	447:456	arg1	pulposus					537:544	cervical disc herniated nucleus pulposus	505:544	cervical disc herniated nucleus pulposus (NP)	505:549	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	82	theme	disc	514:517	arg1	NP					547:548	NP	547:548	NP	547:548	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	82	theme	disc	514:517	arg1	pulposus					537:544	cervical disc herniated nucleus pulposus	505:544	cervical disc herniated nucleus pulposus (NP)	505:549	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	12	83	theme	NP	2156:2157	arg1	structure					2139:2147	the general structure	2127:2147	the general structure of the NP	2127:2157	Moreover, histological staining (H&E staining and Safranin-O staining) showed the general structure of the NP and the distribution of macromolecules.					
36277712	12	83	theme	NP	2156:2157	arg1	distribution					2167:2178	the distribution	2163:2178	the distribution of macromolecules	2163:2196	Moreover, histological staining (H&E staining and Safranin-O staining) showed the general structure of the NP and the distribution of macromolecules.					
36277712	6	84	theme	imaging	1098:1104	arg1	images					1124:1129	the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images	1043:1129	the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images	1043:1129	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	14	85	from	alteration	2467:2476	arg1	structure					2493:2501	structure	2493:2501	structure	2493:2501	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	14	85	from	alteration	2467:2476	arg1	content					2481:2487	content	2481:2487	content	2481:2487	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	10	86	theme	1,064/	1694:1699	arg1	peaks					1685:1689	the two peaks	1677:1689	the two peaks (I 1,064/ I 1,004) in the NP	1677:1718	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	10	86	theme	1,064/	1694:1699	arg1	I					1701:1701	I 1,064/ I 1,004	1692:1707	I 1,064/ I 1,004	1692:1707	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	0	87	from	pulposus	70:77	arg1	Identification					0:13	Identification	0:13	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.	0:142	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.					
36277712	6	88	theme	signal	1184:1189	arg1	intensity					1191:1199	T2 signal intensity	1181:1199	the T2 signal intensity value of NP	1177:1211	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	14	89	from	content	2481:2487	arg1	degree					2457:2462	some degree	2452:2462	some degree of alteration in content and structure	2452:2501	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	10	90	theme	NP	1833:1834	arg1	value					1846:1850	the NP intensity value	1829:1850	the NP intensity value	1829:1850	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	1	91	from	diseases	216:223	arg1	society					235:241	society	235:241	society	235:241	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	14	92	theme	alteration	2467:2476	arg1	degree					2457:2462	some degree	2452:2462	some degree of alteration in content and structure	2452:2501	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	4	93	theme	research	852:859	arg1	purposes					861:868	the above mentioned research purposes	832:868	the above mentioned research purposes	832:868	Methods Our study used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes.					
36277712	12	94	theme	general	2131:2137	arg1	structure					2139:2147	the general structure	2127:2147	the general structure of the NP	2127:2157	Moreover, histological staining (H&E staining and Safranin-O staining) showed the general structure of the NP and the distribution of macromolecules.					
36277712	7	95	theme	and Safranin-O	1312:1325	arg1	staining					1327:1334	and Safranin-O staining	1312:1334	H&E staining and Safranin-O staining	1299:1334	In addition, the structure of the NP samples was evaluated by histological staining (H&E staining and Safranin-O staining).					
36277712	13	96	theme	cervical	2316:2323	arg1	tissue					2333:2338	the cervical disc NP tissue	2312:2338	the cervical disc NP tissue by Raman spectroscopy	2312:2360	Conclusion The present study demonstrated the possibility of characterizing the macromolecular substances inside the cervical disc NP tissue by Raman spectroscopy.					
36277712	9	97	theme	NP	1430:1431	arg1	tissues					1433:1439	28 NP tissues	1427:1439	28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH	1427:1527	Results A total of 28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH were included in this study.					
36277712	11	98	theme	microstructural	1903:1917	arg1	characterization					1919:1934	the microstructural characterization	1899:1934	the microstructural characterization of collagen	1899:1946	For the microstructural characterization of collagen, we found that it may have an essential role in the degenerative process of the intervertebral disc.					
36277712	7	99	theme	H&E	1299:1301	arg1	staining					1303:1310	H&E staining	1299:1310	H&E staining and Safranin-O staining	1299:1334	In addition, the structure of the NP samples was evaluated by histological staining (H&E staining and Safranin-O staining).					
36277712	14	100	theme	positive	2544:2551	arg1	significance					2553:2564	a further positive significance	2534:2564	a further positive significance for the elucidation of CDH's mechanism	2534:2603	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	1	101	theme	Purpose	144:150	arg1	CDH					178:180	CDH	178:180	CDH	178:180	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	1	101	theme	Purpose	144:150	arg1	herniation					166:175	Purpose Cervical disc herniation	144:175	Purpose Cervical disc herniation (CDH)	144:181	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	0	102	theme	patients	82:89	arg1	pulposus					70:77	the nucleus pulposus	58:77	the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy	58:141	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.					
36277712	1	103	theme	disc	161:164	arg1	CDH					178:180	CDH	178:180	CDH	178:180	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	1	103	theme	disc	161:164	arg1	herniation					166:175	Purpose Cervical disc herniation	144:175	Purpose Cervical disc herniation (CDH)	144:181	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	10	104	from	ratio	1668:1672	arg1	NP					1717:1718	the NP	1713:1718	the NP	1713:1718	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	11	105	theme	intervertebral	2028:2041	arg1	disc					2043:2046	the intervertebral disc	2024:2046	the intervertebral disc	2024:2046	For the microstructural characterization of collagen, we found that it may have an essential role in the degenerative process of the intervertebral disc.					
36277712	10	106	theme	negative	1743:1750	arg1	correlation					1752:1762	a significantly negative correlation	1727:1762	a significantly negative correlation with Pfirrmann grade (P < 0.001)	1727:1795	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	0	107	theme	cervical	96:103	arg1	herniation					110:119	cervical disc herniation	96:119	cervical disc herniation	96:119	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.					
36277712	1	108	theme	primary	316:322	arg1	degeneration					264:275	intervertebral disc degeneration	244:275	intervertebral disc degeneration (IVDD)	244:282	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	1	108	theme	primary	316:322	arg1	cause					324:328	its primary cause	312:328	its primary cause	312:328	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	5	109	theme	imaging	922:928	arg1	data					930:933	imaging data	922:933	imaging data	922:933	Firstly, we collected cervical disc NP samples and imaging data by certain inclusion and exclusion criteria.					
36277712	12	110	theme	H&E	2082:2084	arg1	staining					2086:2093	H&E staining	2082:2093	H&E staining	2082:2093	Moreover, histological staining (H&E staining and Safranin-O staining) showed the general structure of the NP and the distribution of macromolecules.					
36277712	14	111	theme	further	2536:2542	arg1	significance					2553:2564	a further positive significance	2534:2564	a further positive significance for the elucidation of CDH's mechanism	2534:2603	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	7	112	dep	staining	1289:1296	arg1	staining					1303:1310	H&E staining	1299:1310	H&E staining and Safranin-O staining	1299:1334	In addition, the structure of the NP samples was evaluated by histological staining (H&E staining and Safranin-O staining).					
36277712	13	113	theme	macromolecular	2279:2292	arg1	substances					2294:2303	the macromolecular substances	2275:2303	the macromolecular substances inside the cervical disc NP tissue by Raman spectroscopy	2275:2360	Conclusion The present study demonstrated the possibility of characterizing the macromolecular substances inside the cervical disc NP tissue by Raman spectroscopy.					
36277712	10	114	from	peaks	1685:1689	arg1	NP					1717:1718	the NP	1713:1718	the NP	1713:1718	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	0	115	from	changes	47:53	arg1	pulposus					70:77	the nucleus pulposus	58:77	the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy	58:141	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.					
36277712	12	116	theme	histological	2059:2070	arg1	staining					2072:2079	histological staining	2059:2079	histological staining (H&E staining and Safranin-O staining)	2059:2118	Moreover, histological staining (H&E staining and Safranin-O staining) showed the general structure of the NP and the distribution of macromolecules.					
36277712	9	117	theme	patients	1472:1479	arg1	two					1459:1461	two	1459:1461	two	1459:1461	Results A total of 28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH were included in this study.					
36277712	9	117	theme	patients	1472:1479	arg1	patients					1472:1479	these patients	1466:1479	these patients	1466:1479	Results A total of 28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH were included in this study.					
36277712	5	118	theme	disc	902:905	arg1	samples					910:916	cervical disc NP samples	893:916	cervical disc NP samples	893:916	Firstly, we collected cervical disc NP samples and imaging data by certain inclusion and exclusion criteria.					
36277712	7	119	theme	samples	1251:1257	arg1	structure					1231:1239	the structure	1227:1239	the structure of the NP samples	1227:1257	In addition, the structure of the NP samples was evaluated by histological staining (H&E staining and Safranin-O staining).					
36277712	11	120	theme	essential	1978:1986	arg1	role					1988:1991	an essential role	1975:1991	an essential role	1975:1991	For the microstructural characterization of collagen, we found that it may have an essential role in the degenerative process of the intervertebral disc.					
36277712	10	121	theme	Raman	1575:1579	arg1	spectroscopy					1581:1592	the Raman spectroscopy scan	1571:1597	the Raman spectroscopy scan	1571:1597	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	0	122	theme	changes	47:53	arg1	Identification					0:13	Identification	0:13	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.	0:142	Identification of compositional and structural changes in the nucleus pulposus of patients with cervical disc herniation by Raman spectroscopy.					
36277712	14	123	theme	macromolecular	2386:2399	arg1	proteoglycans					2420:2432	proteoglycans	2420:2432	proteoglycans	2420:2432	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	14	123	theme	macromolecular	2386:2399	arg1	collagen					2438:2445	collagen	2438:2445	collagen	2438:2445	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	14	123	theme	macromolecular	2386:2399	arg1	substances					2401:2410	macromolecular substances	2386:2410	macromolecular substances such as proteoglycans and collagen	2386:2445	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	3	124	theme	Raman	584:588	arg1	spectra					590:596	Raman spectra	584:596	a validated and convenient Raman spectra technique	557:606	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	2	125	theme	disc	372:375	arg1	degeneration					377:388	intervertebral disc degeneration	357:388	intervertebral disc degeneration	357:388	However, the mechanism of intervertebral disc degeneration is still unclear.					
36277712	11	126	theme	collagen	1939:1946	arg1	characterization					1919:1934	the microstructural characterization	1899:1934	the microstructural characterization of collagen	1899:1946	For the microstructural characterization of collagen, we found that it may have an essential role in the degenerative process of the intervertebral disc.					
36277712	13	127	theme	present	2214:2220	arg1	study					2222:2226	The present study	2210:2226	The present study	2210:2226	Conclusion The present study demonstrated the possibility of characterizing the macromolecular substances inside the cervical disc NP tissue by Raman spectroscopy.					
36277712	9	128	from	total	1418:1422	arg1	patients					1449:1456	26 patients	1446:1456	26 patients (two of these patients were cases that involved two segments) with CDH	1446:1527	Results A total of 28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH were included in this study.					
36277712	6	129	theme	resonance	1088:1096	arg1	MRI					1107:1109	MRI	1107:1109	MRI	1107:1109	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	6	129	theme	resonance	1088:1096	arg1	imaging					1098:1104	the patient's preoperative cervical magnetic resonance imaging	1043:1104	the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images	1043:1129	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	11	130	theme	degenerative	2000:2011	arg1	process					2013:2019	the degenerative process	1996:2019	the degenerative process of the intervertebral disc	1996:2046	For the microstructural characterization of collagen, we found that it may have an essential role in the degenerative process of the intervertebral disc.					
36277712	14	131	contain	has	2530:2532	arg2	significance					2553:2564	a further positive significance	2534:2564	a further positive significance for the elucidation of CDH's mechanism	2534:2603	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	14	131	contain	has	2530:2532	arg1	degeneration					2510:2521	degeneration	2510:2521	degeneration	2510:2521	It also confirmed that macromolecular substances such as proteoglycans and collagen have some degree of alteration in content and structure during degeneration, which has a further positive significance for the elucidation of CDH's mechanism.					
36277712	4	132	theme	in	784:785	arg1	NP					813:814	in vitro intervertebral disc NP	784:814	in vitro intervertebral disc NP	784:814	Methods Our study used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes.					
36277712	9	133	dep	Results	1408:1414	arg1	included					1534:1541	included	1534:1541	were included in this study	1529:1555	Results A total of 28 NP tissues from 26 patients (two of these patients were cases that involved two segments) with CDH were included in this study.					
36277712	5	134	theme	inclusion	946:954	arg1	criteria					970:977	certain inclusion and exclusion criteria	938:977	certain inclusion and exclusion criteria	938:977	Firstly, we collected cervical disc NP samples and imaging data by certain inclusion and exclusion criteria.					
36277712	6	135	theme	cervical	1070:1077	arg1	MRI					1107:1109	MRI	1107:1109	MRI	1107:1109	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	6	135	theme	cervical	1070:1077	arg1	imaging					1098:1104	the patient's preoperative cervical magnetic resonance imaging	1043:1104	the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images	1043:1129	Then, we graded the NP of the responsible segment according to the patient's preoperative cervical magnetic resonance imaging (MRI) T2-weighted images by Pfirrmann grading criteria while measuring the T2 signal intensity value of NP.					
36277712	3	136	dep	components	447:456	arg1	the					443:445	the	443:445	the	443:445	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	137	theme	histological	612:623	arg1	methods					625:631	histological methods	612:631	histological methods	612:631	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	10	138	theme	proteoglycans	1624:1636	arg1	content					1613:1619	the relative content	1600:1619	the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP	1600:1718	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	1	139	theme	spinal	209:214	arg1	diseases					216:223	the most common spinal diseases	193:223	the most common spinal diseases in modern society	193:241	Purpose Cervical disc herniation (CDH) is one of the most common spinal diseases in modern society; intervertebral disc degeneration (IVDD) has long been considered as its primary cause.					
36277712	10	140	from	NP	1717:1718	arg1	ratio					1668:1672	the ratio	1664:1672	the ratio of the two peaks (I 1,064/ I 1,004) in the NP	1664:1718	According to the Raman spectroscopy scan, the relative content of proteoglycans which is characterized by the ratio of the two peaks (I 1,064/ I 1,004) in the NP showed a significantly negative correlation with Pfirrmann grade (P < 0.001), while the collagen content and the NP intensity value showed a positive correlation (P < 0.001).					
36277712	5	141	theme	exclusion	960:968	arg1	criteria					970:977	certain inclusion and exclusion criteria	938:977	certain inclusion and exclusion criteria	938:977	Firstly, we collected cervical disc NP samples and imaging data by certain inclusion and exclusion criteria.					
36277712	4	142	theme	Raman	751:755	arg1	spectroscopy					757:768	Raman spectroscopy	751:768	Raman spectroscopy combined with in vitro intervertebral disc NP	751:814	Methods Our study used a burgeoning technique of Raman spectroscopy combined with in vitro intervertebral disc NP to characterize the above mentioned research purposes.					
36277712	3	143	theme	proteoglycan	476:487	arg1	structures					462:471	structures	462:471	structures	462:471	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
36277712	3	143	theme	proteoglycan	476:487	arg1	components					447:456	components	447:456	components	447:456	The aim of the study is to examine the components and structures of proteoglycan and collagen in cervical disc herniated nucleus pulposus (NP) using a validated and convenient Raman spectra technique and histological methods to further elucidate the mechanism of IVDD at the microscopic level.					
35926618	5	0	from	temperatures	1117:1128	arg1	distributions					1100:1112	equilibrium distributions	1088:1112	equilibrium distributions at temperatures between 20 and 80 °C	1088:1149	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	1	1	theme	adverse	364:370	arg1	effects					379:385	adverse health effects	364:385	adverse health effects	364:385	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	5	2	dep	22	1208:1209	arg1	to					1205:1206	to	1205:1206	to	1205:1206	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	5	3	from	behaviour	903:911	arg1	materials					988:996	cellulose acetate (CA)-based materials	959:996	cellulose acetate (CA)-based materials	959:996	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	6	4	from	experiments	1430:1440	arg1	measurements					1399:1410	measurements	1399:1410	measurements from 130 distinct experiments	1399:1440	Additionally, we propose a model to describe and quantify the effect of temperature and plasticiser composition, with model parameters being estimated using non-linear regression and measurements from 130 distinct experiments.					
35926618	6	4	from	experiments	1430:1440	arg1	regression					1384:1393	non-linear regression	1373:1393	non-linear regression	1373:1393	Additionally, we propose a model to describe and quantify the effect of temperature and plasticiser composition, with model parameters being estimated using non-linear regression and measurements from 130 distinct experiments.					
35926618	0	5	from	distribution	12:23	arg1	Modelling					96:104	Modelling	96:104	Modelling	96:104	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	5	from	distribution	12:23	arg1	estimation					120:129	parameter estimation	110:129	parameter estimation	110:129	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	5	from	distribution	12:23	arg1	materials					85:93	cellulose acetate-based materials	61:93	cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects	61:168	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	6	dep	temperature	134:144	arg1	effects					162:168	effects	162:168	effects	162:168	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	1	7	theme	indoor	273:278	arg1	environments					280:291	indoor environments	273:291	indoor environments	273:291	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	0	8	theme	parameter	110:118	arg1	estimation					120:129	parameter estimation	110:129	parameter estimation	110:129	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	8	theme	parameter	110:118	arg1	materials					85:93	cellulose acetate-based materials	61:93	cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects	61:168	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	7	9	theme	DEP	1530:1532	arg1	migration					1517:1525	the migration	1513:1525	the migration of DEP from CA-based materials	1513:1556	Finally, we assess the suitability of our developed model to simulate the migration of DEP from CA-based materials.					
35926618	3	10	theme	Partition	601:609	arg1	coefficients					611:622	Partition coefficients	601:622	Partition coefficients	601:622	Partition coefficients are also of fundamental importance for describing degradation phenomena associated with plasticiser loss from polymeric materials.					
35926618	5	11	theme	wt	1211:1212	arg1	%					1213:1213	6 to 22 wt%	1203:1213	6 to 22 wt%	1203:1213	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	5	12	theme	CA	1161:1162	arg1	samples					1164:1170	CA samples	1161:1170	CA samples with DEP contents ranging from 6 to 22 wt%	1161:1213	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	6	13	theme	plasticiser	1304:1314	arg1	effect					1278:1283	the effect	1274:1283	the effect of temperature and plasticiser composition	1274:1326	Additionally, we propose a model to describe and quantify the effect of temperature and plasticiser composition, with model parameters being estimated using non-linear regression and measurements from 130 distinct experiments.					
35926618	7	14	theme	model	1495:1499	arg1	suitability					1466:1476	the suitability	1462:1476	the suitability of our developed model to simulate the migration of DEP from CA-based materials	1462:1556	Finally, we assess the suitability of our developed model to simulate the migration of DEP from CA-based materials.					
35926618	6	15	theme	temperature	1288:1298	arg1	effect					1278:1283	the effect	1274:1283	the effect of temperature and plasticiser composition	1274:1326	Additionally, we propose a model to describe and quantify the effect of temperature and plasticiser composition, with model parameters being estimated using non-linear regression and measurements from 130 distinct experiments.					
35926618	5	16	theme	first	1006:1010	arg1	time					1012:1015	the first time	1002:1015	the first time	1002:1015	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	5	17	theme	diethyl	920:926	arg1	plasticiser					944:954	the diethyl phthalate (DEP) plasticiser	916:954	the diethyl phthalate (DEP) plasticiser	916:954	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	6	18	dep	temperature	1288:1298	arg1	composition					1316:1326	composition	1316:1326	composition	1316:1326	Additionally, we propose a model to describe and quantify the effect of temperature and plasticiser composition, with model parameters being estimated using non-linear regression and measurements from 130 distinct experiments.					
35926618	5	19	theme	DEP	939:941	arg1	plasticiser					944:954	the diethyl phthalate (DEP) plasticiser	916:954	the diethyl phthalate (DEP) plasticiser	916:954	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	3	20	from	materials	744:752	arg1	loss					724:727	plasticiser loss	712:727	plasticiser loss from polymeric materials	712:752	Partition coefficients are also of fundamental importance for describing degradation phenomena associated with plasticiser loss from polymeric materials.					
35926618	2	21	theme	surrounding	506:516	arg1	atmosphere					518:527	the surrounding atmosphere	502:527	the surrounding atmosphere	502:527	In this context, the partition coefficient of phthalates between indoor built materials and/or consumer goods and the surrounding atmosphere represents a key parameter for determining concentration distributions.					
35926618	0	22	theme	Equilibrium	0:10	arg1	distribution					12:23	Equilibrium distribution	0:23	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.	0:169	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	2	23	theme	partition	409:417	arg1	coefficient					419:429	the partition coefficient	405:429	the partition coefficient of phthalates between indoor built materials and/or consumer goods	405:496	In this context, the partition coefficient of phthalates between indoor built materials and/or consumer goods and the surrounding atmosphere represents a key parameter for determining concentration distributions.					
35926618	2	24	theme	consumer	483:490	arg1	goods					492:496	consumer goods	483:496	consumer goods	483:496	In this context, the partition coefficient of phthalates between indoor built materials and/or consumer goods and the surrounding atmosphere represents a key parameter for determining concentration distributions.					
35926618	5	25	theme	DEP	1177:1179	arg1	contents					1181:1188	DEP contents	1177:1188	DEP contents ranging from 6 to 22 wt%	1177:1213	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	7	26	from	materials	1548:1556	arg1	migration					1517:1525	the migration	1513:1525	the migration of DEP from CA-based materials	1513:1556	Finally, we assess the suitability of our developed model to simulate the migration of DEP from CA-based materials.					
35926618	5	27	theme	plasticiser	944:954	arg1	behaviour					903:911	the partitioning behaviour	886:911	the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials	886:996	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	1	28	theme	health	372:377	arg1	effects					379:385	adverse health effects	364:385	adverse health effects	364:385	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	7	29	theme	developed	1485:1493	arg1	model					1495:1499	our developed model	1481:1499	our developed model	1481:1499	Finally, we assess the suitability of our developed model to simulate the migration of DEP from CA-based materials.					
35926618	2	30	theme	concentration	572:584	arg1	distributions					586:598	concentration distributions	572:598	concentration distributions	572:598	In this context, the partition coefficient of phthalates between indoor built materials and/or consumer goods and the surrounding atmosphere represents a key parameter for determining concentration distributions.					
35926618	7	31	theme	CA-based	1539:1546	arg1	materials					1548:1556	CA-based materials	1539:1556	CA-based materials	1539:1556	Finally, we assess the suitability of our developed model to simulate the migration of DEP from CA-based materials.					
35926618	1	32	dep	transport	189:197	arg1	the					185:187	the	185:187	the	185:187	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	4	33	theme	environmental	844:856	arg1	conditions					858:867	environmental conditions	844:867	environmental conditions	844:867	However, this key parameter has only been determined for a limited number of systems and environmental conditions.					
35926618	1	34	theme	semi-volatile	211:223	arg1	compounds					233:241	semi-volatile organic compounds	211:241	semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments	211:291	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	1	34	theme	semi-volatile	211:223	arg1	phthalates					259:268	phthalates	259:268	phthalates in indoor environments	259:291	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	1	34	theme	semi-volatile	211:223	arg1	SVOCs					244:248	SVOCs	244:248	SVOCs	244:248	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	0	35	theme	diethyl	28:34	arg1	plasticiser					46:56	diethyl phthalate plasticiser	28:56	diethyl phthalate plasticiser	28:56	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	4	36	theme	limited	814:820	arg1	number					822:827	a limited number	812:827	a limited number of systems and environmental conditions	812:867	However, this key parameter has only been determined for a limited number of systems and environmental conditions.					
35926618	0	37	dep	materials	85:93	arg1	Modelling					96:104	Modelling	96:104	Modelling	96:104	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	37	dep	materials	85:93	arg1	estimation					120:129	parameter estimation	110:129	parameter estimation	110:129	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	37	dep	materials	85:93	arg1	materials					85:93	cellulose acetate-based materials	61:93	cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects	61:168	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	1	38	theme	organic	225:231	arg1	compounds					233:241	semi-volatile organic compounds	211:241	semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments	211:291	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	1	38	theme	organic	225:231	arg1	phthalates					259:268	phthalates	259:268	phthalates in indoor environments	259:291	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	1	38	theme	organic	225:231	arg1	SVOCs					244:248	SVOCs	244:248	SVOCs	244:248	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	2	39	theme	built	460:464	arg1	materials					466:474	indoor built materials	453:474	indoor built materials	453:474	In this context, the partition coefficient of phthalates between indoor built materials and/or consumer goods and the surrounding atmosphere represents a key parameter for determining concentration distributions.					
35926618	0	40	theme	temperature	134:144	arg1	Modelling					96:104	Modelling	96:104	Modelling	96:104	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	40	theme	temperature	134:144	arg1	estimation					120:129	parameter estimation	110:129	parameter estimation	110:129	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	40	theme	temperature	134:144	arg1	materials					85:93	cellulose acetate-based materials	61:93	cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects	61:168	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	3	41	theme	polymeric	734:742	arg1	materials					744:752	polymeric materials	734:752	polymeric materials	734:752	Partition coefficients are also of fundamental importance for describing degradation phenomena associated with plasticiser loss from polymeric materials.					
35926618	1	42	theme	compounds	233:241	arg1	fate					203:206	fate	203:206	fate	203:206	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	1	42	theme	compounds	233:241	arg1	transport					189:197	transport	189:197	transport	189:197	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	0	43	theme	plasticiser	46:56	arg1	distribution					12:23	Equilibrium distribution	0:23	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.	0:169	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	6	44	theme	non-linear	1373:1382	arg1	regression					1384:1393	non-linear regression	1373:1393	non-linear regression	1373:1393	Additionally, we propose a model to describe and quantify the effect of temperature and plasticiser composition, with model parameters being estimated using non-linear regression and measurements from 130 distinct experiments.					
35926618	5	45	theme	phthalate	928:936	arg1	plasticiser					944:954	the diethyl phthalate (DEP) plasticiser	916:954	the diethyl phthalate (DEP) plasticiser	916:954	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	2	46	theme	phthalates	434:443	arg1	atmosphere					518:527	the surrounding atmosphere	502:527	the surrounding atmosphere	502:527	In this context, the partition coefficient of phthalates between indoor built materials and/or consumer goods and the surrounding atmosphere represents a key parameter for determining concentration distributions.					
35926618	2	46	theme	phthalates	434:443	arg1	coefficient					419:429	the partition coefficient	405:429	the partition coefficient of phthalates between indoor built materials and/or consumer goods	405:496	In this context, the partition coefficient of phthalates between indoor built materials and/or consumer goods and the surrounding atmosphere represents a key parameter for determining concentration distributions.					
35926618	0	47	theme	phthalate	36:44	arg1	plasticiser					46:56	diethyl phthalate plasticiser	28:56	diethyl phthalate plasticiser	28:56	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	48	theme	composition	150:160	arg1	Modelling					96:104	Modelling	96:104	Modelling	96:104	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	48	theme	composition	150:160	arg1	estimation					120:129	parameter estimation	110:129	parameter estimation	110:129	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	48	theme	composition	150:160	arg1	materials					85:93	cellulose acetate-based materials	61:93	cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects	61:168	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	3	49	theme	degradation	674:684	arg1	phenomena					686:694	degradation phenomena	674:694	degradation phenomena associated with plasticiser loss from polymeric materials	674:752	Partition coefficients are also of fundamental importance for describing degradation phenomena associated with plasticiser loss from polymeric materials.					
35926618	6	50	theme	model	1334:1338	arg1	parameters					1340:1349	model parameters	1334:1349	model parameters	1334:1349	Additionally, we propose a model to describe and quantify the effect of temperature and plasticiser composition, with model parameters being estimated using non-linear regression and measurements from 130 distinct experiments.					
35926618	0	51	theme	cellulose	61:69	arg1	Modelling					96:104	Modelling	96:104	Modelling	96:104	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	51	theme	cellulose	61:69	arg1	estimation					120:129	parameter estimation	110:129	parameter estimation	110:129	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	51	theme	cellulose	61:69	arg1	materials					85:93	cellulose acetate-based materials	61:93	cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects	61:168	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	1	52	theme	human	334:338	arg1	exposure					340:347	human exposure	334:347	human exposure	334:347	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	6	53	theme	distinct	1421:1428	arg1	experiments					1430:1440	130 distinct experiments	1417:1440	130 distinct experiments	1417:1440	Additionally, we propose a model to describe and quantify the effect of temperature and plasticiser composition, with model parameters being estimated using non-linear regression and measurements from 130 distinct experiments.					
35926618	1	54	theme	exposure	340:347	arg1	levels					324:329	levels	324:329	levels of human exposure	324:347	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	1	55	from	phthalates	259:268	arg1	environments					280:291	indoor environments	273:291	indoor environments	273:291	Understanding the transport and fate of semi-volatile organic compounds (SVOCs) such as phthalates in indoor environments is fundamental for quantifying levels of human exposure and preventing adverse health effects.					
35926618	5	56	dep	assess	879:884	arg1	determining					1018:1028	determining	1018:1028	determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C	1018:1149	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	5	56	dep	assess	879:884	arg1	using					1155:1159	using	1155:1159	using CA samples with DEP contents ranging from 6 to 22 wt%	1155:1213	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	5	57	theme	-based	981:986	arg1	materials					988:996	cellulose acetate (CA)-based materials	959:996	cellulose acetate (CA)-based materials	959:996	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	5	58	theme	temperature	1045:1055	arg1	effects					1034:1040	the effects	1030:1040	the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C	1030:1149	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	5	59	theme	equilibrium	1088:1098	arg1	distributions					1100:1112	equilibrium distributions	1088:1112	equilibrium distributions at temperatures between 20 and 80 °C	1088:1149	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	4	60	theme	systems	832:838	arg1	number					822:827	a limited number	812:827	a limited number of systems and environmental conditions	812:867	However, this key parameter has only been determined for a limited number of systems and environmental conditions.					
35926618	4	61	theme	conditions	858:867	arg1	number					822:827	a limited number	812:827	a limited number of systems and environmental conditions	812:867	However, this key parameter has only been determined for a limited number of systems and environmental conditions.					
35926618	3	62	theme	plasticiser	712:722	arg1	loss					724:727	plasticiser loss	712:727	plasticiser loss from polymeric materials	712:752	Partition coefficients are also of fundamental importance for describing degradation phenomena associated with plasticiser loss from polymeric materials.					
35926618	4	63	theme	key	769:771	arg1	parameter					773:781	this key parameter	764:781	this key parameter	764:781	However, this key parameter has only been determined for a limited number of systems and environmental conditions.					
35926618	2	64	theme	key	542:544	arg1	parameter					546:554	a key parameter	540:554	a key parameter for determining concentration distributions	540:598	In this context, the partition coefficient of phthalates between indoor built materials and/or consumer goods and the surrounding atmosphere represents a key parameter for determining concentration distributions.					
35926618	5	65	theme	plasticiser	1061:1071	arg1	composition					1073:1083	plasticiser composition	1061:1083	plasticiser composition	1061:1083	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	2	66	theme	indoor	453:458	arg1	materials					466:474	indoor built materials	453:474	indoor built materials	453:474	In this context, the partition coefficient of phthalates between indoor built materials and/or consumer goods and the surrounding atmosphere represents a key parameter for determining concentration distributions.					
35926618	5	67	with	samples	1164:1170	arg1	contents					1181:1188	DEP contents	1177:1188	DEP contents ranging from 6 to 22 wt%	1177:1213	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	0	68	theme	acetate-based	71:83	arg1	Modelling					96:104	Modelling	96:104	Modelling	96:104	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	68	theme	acetate-based	71:83	arg1	estimation					120:129	parameter estimation	110:129	parameter estimation	110:129	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	0	68	theme	acetate-based	71:83	arg1	materials					85:93	cellulose acetate-based materials	61:93	cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects	61:168	Equilibrium distribution of diethyl phthalate plasticiser in cellulose acetate-based materials: Modelling and parameter estimation of temperature and composition effects.					
35926618	5	69	theme	composition	1073:1083	arg1	effects					1034:1040	the effects	1030:1040	the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C	1030:1149	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	3	70	theme	fundamental	636:646	arg1	importance					648:657	fundamental importance	636:657	fundamental importance	636:657	Partition coefficients are also of fundamental importance for describing degradation phenomena associated with plasticiser loss from polymeric materials.					
35926618	5	71	from	effects	1034:1040	arg1	distributions					1100:1112	equilibrium distributions	1088:1112	equilibrium distributions at temperatures between 20 and 80 °C	1088:1149	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35926618	5	72	theme	partitioning	890:901	arg1	behaviour					903:911	the partitioning behaviour	886:911	the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials	886:996	Here, we assess the partitioning behaviour of the diethyl phthalate (DEP) plasticiser in cellulose acetate (CA)-based materials for the first time, determining the effects of temperature and plasticiser composition on equilibrium distributions at temperatures between 20 and 80 °C and using CA samples with DEP contents ranging from 6 to 22 wt%.					
35349901	1	0	theme	cake	329:332	arg1	development					334:344	sponge cake development	322:344	sponge cake development	322:344	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	4	1	theme	aldehydes	908:916	arg1	mixture					877:883	a more complex mixture	862:883	a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes	862:977	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	4	2	theme	Strecker	899:906	arg1	aldehydes					908:916	Strecker aldehydes	899:916	Strecker aldehydes	899:916	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	3	3	theme	batter	705:710	arg1	structure					712:720	batter structure	705:720	batter structure	705:720	This was due more to the inactivation of lipoxygenase during flour fractionation than to differences in batter structure.					
35349901	1	4	theme	development	334:344	arg1	steps					313:317	the different steps	299:317	the different steps of sponge cake development	299:344	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	4	5	theme	complex	869:875	arg1	mixture					877:883	a more complex mixture	862:883	a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes	862:977	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	5	6	theme	purified	1066:1073	arg1	fractions					1079:1087	purified pea fractions	1066:1087	purified pea fractions	1066:1087	These findings confirm that using purified pea fractions can create high-quality products with an attractive composition.					
35349901	2	7	theme	pea	509:511	arg1	protein					513:519	purified pea starch and pea protein	485:519	purified pea starch and pea protein	485:519	While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors.					
35349901	0	8	from	Applicability	0:12	arg1	products					42:49	baked products	36:49	baked products	36:49	Applicability of pea ingredients in baked products: Links between formulation, reactivity potential and physicochemical properties.					
35349901	4	9	theme	pyrazines	888:896	arg1	mixture					877:883	a more complex mixture	862:883	a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes	862:977	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	0	10	theme	reactivity	79:88	arg1	potential					90:98	reactivity potential	79:98	reactivity potential	79:98	Applicability of pea ingredients in baked products: Links between formulation, reactivity potential and physicochemical properties.					
35349901	2	11	theme	green-beany	578:588	arg1	off-odors					590:598	known green-beany off-odors	572:598	known green-beany off-odors	572:598	While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors.					
35349901	2	12	theme	starch	498:503	arg1	protein					513:519	purified pea starch and pea protein	485:519	purified pea starch and pea protein	485:519	While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors.					
35349901	1	13	theme	pea	358:360	arg1	flour					362:366	pea flour	358:366	pea flour	358:366	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	2	14	theme	pea	494:496	arg1	starch					498:503	pea starch	494:503	pea starch	494:503	While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors.					
35349901	4	15	theme	pea	1006:1008	arg1	flour					1010:1014	pea flour	1006:1014	pea flour	1006:1014	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	4	16	theme	wheat	1019:1023	arg1	flour					1025:1029	wheat flour	1019:1029	wheat flour	1019:1029	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	4	17	with	compounds	930:938	arg1	malty					955:959	potential malty	945:959	potential malty	945:959	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	4	17	with	compounds	930:938	arg1	notes					973:977	roasted notes	965:977	roasted notes	965:977	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	0	18	theme	physicochemical	104:118	arg1	properties					120:129	physicochemical properties	104:129	physicochemical properties	104:129	Applicability of pea ingredients in baked products: Links between formulation, reactivity potential and physicochemical properties.					
35349901	5	19	theme	high-quality	1100:1111	arg1	products					1113:1120	high-quality products	1100:1120	high-quality products	1100:1120	These findings confirm that using purified pea fractions can create high-quality products with an attractive composition.					
35349901	1	20	theme	purified	182:189	arg1	starch					208:213	starch	208:213	starch	208:213	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	1	20	theme	purified	182:189	arg1	isolate					227:233	protein isolate	219:233	protein isolate	219:233	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	1	20	theme	purified	182:189	arg1	ingredients					195:205	purified pea ingredients	182:205	purified pea ingredients (starch and protein isolate)	182:234	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	2	21	theme	lipid	427:431	arg1	oxidation					433:441	lipid oxidation	427:441	lipid oxidation during batter beating	427:463	While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors.					
35349901	4	22	theme	fractionated	732:743	arg1	ingredients					745:755	fractionated ingredients	732:755	fractionated ingredients	732:755	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	1	23	theme	pea	191:193	arg1	starch					208:213	starch	208:213	starch	208:213	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	1	23	theme	pea	191:193	arg1	isolate					227:233	protein isolate	219:233	protein isolate	219:233	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	1	23	theme	pea	191:193	arg1	ingredients					195:205	purified pea ingredients	182:205	purified pea ingredients (starch and protein isolate)	182:234	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	0	24	theme	ingredients	21:31	arg1	Applicability					0:12	Applicability	0:12	Applicability of pea ingredients in baked products: Links between formulation, reactivity potential and physicochemical properties.	0:130	Applicability of pea ingredients in baked products: Links between formulation, reactivity potential and physicochemical properties.					
35349901	1	25	theme	volatile	273:280	arg1	compounds					282:290	volatile compounds	273:290	volatile compounds	273:290	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	2	26	theme	fewer	543:547	arg1	markers					559:565	significantly fewer oxidation markers	529:565	significantly fewer oxidation markers	529:565	While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors.					
35349901	4	27	with	aldehydes	908:916	arg1	malty					955:959	potential malty	945:959	potential malty	945:959	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	4	27	with	aldehydes	908:916	arg1	notes					973:977	roasted notes	965:977	roasted notes	965:977	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	1	28	theme	ingredients	195:205	arg1	applicability					165:177	the applicability	161:177	the applicability of purified pea ingredients (starch and protein isolate)	161:234	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	0	29	theme	pea	17:19	arg1	ingredients					21:31	pea ingredients	17:31	pea ingredients	17:31	Applicability of pea ingredients in baked products: Links between formulation, reactivity potential and physicochemical properties.					
35349901	5	30	theme	attractive	1130:1139	arg1	composition					1141:1151	an attractive composition	1127:1151	an attractive composition	1127:1151	These findings confirm that using purified pea fractions can create high-quality products with an attractive composition.					
35349901	4	31	theme	furanic	922:928	arg1	compounds					930:938	furanic compounds	922:938	furanic compounds	922:938	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	1	32	theme	wheat	372:376	arg1	flour					378:382	wheat flour	372:382	wheat flour	372:382	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	0	33	theme	baked	36:40	arg1	products					42:49	baked products	36:49	baked products	36:49	Applicability of pea ingredients in baked products: Links between formulation, reactivity potential and physicochemical properties.					
35349901	4	34	theme	potential	945:953	arg1	malty					955:959	potential malty	945:959	potential malty	945:959	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	3	35	theme	lipoxygenase	642:653	arg1	inactivation					626:637	the inactivation	622:637	the inactivation of lipoxygenase	622:653	This was due more to the inactivation of lipoxygenase during flour fractionation than to differences in batter structure.					
35349901	3	36	from	differences	690:700	arg1	structure					712:720	batter structure	705:720	batter structure	705:720	This was due more to the inactivation of lipoxygenase during flour fractionation than to differences in batter structure.					
35349901	2	37	theme	protein	513:519	arg1	combination					470:480	the combination	466:480	the combination of purified pea starch and pea protein	466:519	While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors.					
35349901	2	38	theme	pea	391:393	arg1	flour					395:399	pea flour	391:399	pea flour	391:399	While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors.					
35349901	2	39	theme	batter	450:455	arg1	beating					457:463	batter beating	450:463	batter beating	450:463	While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors.					
35349901	1	40	theme	different	303:311	arg1	steps					313:317	the different steps	299:317	the different steps of sponge cake development	299:344	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	2	41	theme	known	572:576	arg1	off-odors					590:598	known green-beany off-odors	572:598	known green-beany off-odors	572:598	While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors.					
35349901	4	42	theme	compounds	930:938	arg1	mixture					877:883	a more complex mixture	862:883	a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes	862:977	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	4	43	with	pyrazines	888:896	arg1	malty					955:959	potential malty	945:959	potential malty	945:959	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	4	43	with	pyrazines	888:896	arg1	notes					973:977	roasted notes	965:977	roasted notes	965:977	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	4	44	theme	Maillard	799:806	arg1	reaction					808:815	the Maillard reaction	795:815	the Maillard reaction	795:815	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35349901	1	45	theme	protein	219:225	arg1	isolate					227:233	protein isolate	219:233	protein isolate	219:233	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	1	45	theme	protein	219:225	arg1	ingredients					195:205	purified pea ingredients	182:205	purified pea ingredients (starch and protein isolate)	182:234	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	2	46	theme	oxidation	549:557	arg1	markers					559:565	significantly fewer oxidation markers	529:565	significantly fewer oxidation markers	529:565	While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors.					
35349901	5	47	theme	pea	1075:1077	arg1	fractions					1079:1087	purified pea fractions	1066:1087	purified pea fractions	1066:1087	These findings confirm that using purified pea fractions can create high-quality products with an attractive composition.					
35349901	2	48	theme	purified	485:492	arg1	protein					513:519	purified pea starch and pea protein	485:519	purified pea starch and pea protein	485:519	While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors.					
35349901	3	49	theme	flour	662:666	arg1	fractionation					668:680	flour fractionation	662:680	flour fractionation	662:680	This was due more to the inactivation of lipoxygenase during flour fractionation than to differences in batter structure.					
35349901	1	50	dep	ingredients	195:205	arg1	starch					208:213	starch	208:213	starch	208:213	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	1	50	dep	ingredients	195:205	arg1	isolate					227:233	protein isolate	219:233	protein isolate	219:233	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	1	50	dep	ingredients	195:205	arg1	ingredients					195:205	purified pea ingredients	182:205	purified pea ingredients (starch and protein isolate)	182:234	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	1	51	theme	sponge	322:327	arg1	development					334:344	sponge cake development	322:344	sponge cake development	322:344	This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour.					
35349901	0	52	dep	Applicability	0:12	arg1	Links					52:56	Links	52:56	Applicability of pea ingredients in baked products: Links between formulation, reactivity potential and physicochemical properties.	0:130	Applicability of pea ingredients in baked products: Links between formulation, reactivity potential and physicochemical properties.					
35349901	4	53	theme	roasted	965:971	arg1	notes					973:977	roasted notes	965:977	roasted notes	965:977	However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour.					
35396734	0	0	from	microencapsulation	11:28	arg1	matrix					39:44	dairy matrix	33:44	dairy matrix	33:44	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid astaxanthin.					
35396734	6	1	theme	rich	690:693	arg1	product					682:688	A dairy product	674:688	A dairy product rich in Asta-C22:6 with high bioavailability	674:733	A dairy product rich in Asta-C22:6 with high bioavailability was designed by measuring quality characteristics, sensory evaluation and in vivo experiments.					
35396734	12	2	theme	Asta-C22:6	1593:1602	arg1	application					1578:1588	the application	1574:1588	the application of Asta-C22:6	1574:1602	It provides an experimental basis for the application of Asta-C22:6 and the development of functional dairy products.					
35396734	7	3	theme	Asta-C22:6	990:999	arg1	1 g L-1					1015:1021	1 g L-1	1015:1021	1 g L-1	1015:1021	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	7	3	theme	Asta-C22:6	990:999	arg1	microcapsule					1001:1012	Asta-C22:6 microcapsule	990:1012	Asta-C22:6 microcapsule (1 g L-1 ) yogurt	990:1030	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	8	4	theme	yogurt	1101:1106	arg1	bioavailability					1058:1072	The bioavailability	1054:1072	The bioavailability of Asta-C22:6 microcapsule yogurt	1054:1106	The bioavailability of Asta-C22:6 microcapsule yogurt was further evaluated.					
35396734	9	5	theme	Cmax	1251:1254	arg1	= 0.31 μg mL-1					1256:1269	Cmax = 0.31 μg mL-1	1251:1269	Cmax = 0.31 μg mL-1	1251:1269	After a single oral dose in mice, the bioavailability of Asta-C22:6 microcapsule in yogurt was significantly increased (Cmax = 0.31 μg mL-1 , AUC0-T = 3.20 h μg mL-1 ).					
35396734	9	5	theme	Cmax	1251:1254	arg1	= 3.20 h μg mL-1					1280:1295	AUC0-T = 3.20 h μg mL-1	1273:1295	AUC0-T = 3.20 h μg mL-1	1273:1295	After a single oral dose in mice, the bioavailability of Asta-C22:6 microcapsule in yogurt was significantly increased (Cmax = 0.31 μg mL-1 , AUC0-T = 3.20 h μg mL-1 ).					
35396734	6	6	from	Asta-C22:6	698:707	arg1	rich					690:693	rich	690:693	rich	690:693	A dairy product rich in Asta-C22:6 with high bioavailability was designed by measuring quality characteristics, sensory evaluation and in vivo experiments.					
35396734	0	7	theme	docosahexaenoic	100:114	arg1	acid					116:119	docosahexaenoic acid	100:119	docosahexaenoic acid	100:119	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid astaxanthin.					
35396734	0	8	from	Effects	0:6	arg1	matrix					39:44	dairy matrix	33:44	dairy matrix	33:44	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid astaxanthin.					
35396734	0	8	from	Effects	0:6	arg1	bioavailability					81:95	bioavailability	81:95	bioavailability	81:95	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid astaxanthin.					
35396734	0	8	from	Effects	0:6	arg1	characteristics					61:75	quality characteristics	53:75	quality characteristics	53:75	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid astaxanthin.					
35396734	9	9	theme	single	1139:1144	arg1	dose					1151:1154	a single oral dose	1137:1154	a single oral dose in mice	1137:1162	After a single oral dose in mice, the bioavailability of Asta-C22:6 microcapsule in yogurt was significantly increased (Cmax = 0.31 μg mL-1 , AUC0-T = 3.20 h μg mL-1 ).					
35396734	7	10	theme	water	944:948	arg1	content					950:956	water content	944:956	water content (4%)	944:961	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	7	10	theme	water	944:948	arg1	%					960:960	4%	959:960	4%	959:960	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	12	11	theme	dairy	1638:1642	arg1	products					1644:1651	functional dairy products	1627:1651	functional dairy products	1627:1651	It provides an experimental basis for the application of Asta-C22:6 and the development of functional dairy products.					
35396734	4	12	theme	whey	473:476	arg1	WPI					495:497	WPI	495:497	WPI	495:497	In this study, Asta-C22:6 microemulsion and microcapsule using whey protein isolate (WPI) and hydroxypropyl-β-cyclodextrin (HPβ-CD) as composite wall material were prepared.					
35396734	4	12	theme	whey	473:476	arg1	isolate					486:492	whey protein isolate	473:492	whey protein isolate (WPI)	473:498	In this study, Asta-C22:6 microemulsion and microcapsule using whey protein isolate (WPI) and hydroxypropyl-β-cyclodextrin (HPβ-CD) as composite wall material were prepared.					
35396734	10	13	theme	Asta	1420:1423	arg1	bioavailability					1401:1415	bioavailability	1401:1415	bioavailability	1401:1415	CONCLUSION We successfully prepared Asta-C22:6 microcapsule yogurt, which improved the stability and bioavailability of Asta.					
35396734	10	13	theme	Asta	1420:1423	arg1	stability					1387:1395	stability	1387:1395	stability	1387:1395	CONCLUSION We successfully prepared Asta-C22:6 microcapsule yogurt, which improved the stability and bioavailability of Asta.					
35396734	6	14	theme	sensory	786:792	arg1	evaluation					794:803	sensory evaluation	786:803	sensory evaluation	786:803	A dairy product rich in Asta-C22:6 with high bioavailability was designed by measuring quality characteristics, sensory evaluation and in vivo experiments.					
35396734	7	15	theme	higher	903:908	arg1	efficiency					924:933	a higher encapsulation efficiency	901:933	a higher encapsulation efficiency (72.5%)	901:941	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	7	15	theme	higher	903:908	arg1	%					940:940	72.5%	936:940	72.5%	936:940	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	9	16	from	bioavailability	1169:1183	arg1	yogurt					1215:1220	yogurt	1215:1220	yogurt	1215:1220	After a single oral dose in mice, the bioavailability of Asta-C22:6 microcapsule in yogurt was significantly increased (Cmax = 0.31 μg mL-1 , AUC0-T = 3.20 h μg mL-1 ).					
35396734	9	17	theme	AUC0-T	1273:1278	arg1	= 0.31 μg mL-1					1256:1269	Cmax = 0.31 μg mL-1	1251:1269	Cmax = 0.31 μg mL-1	1251:1269	After a single oral dose in mice, the bioavailability of Asta-C22:6 microcapsule in yogurt was significantly increased (Cmax = 0.31 μg mL-1 , AUC0-T = 3.20 h μg mL-1 ).					
35396734	9	17	theme	AUC0-T	1273:1278	arg1	= 3.20 h μg mL-1					1280:1295	AUC0-T = 3.20 h μg mL-1	1273:1295	AUC0-T = 3.20 h μg mL-1	1273:1295	After a single oral dose in mice, the bioavailability of Asta-C22:6 microcapsule in yogurt was significantly increased (Cmax = 0.31 μg mL-1 , AUC0-T = 3.20 h μg mL-1 ).					
35396734	5	18	theme	dairy	609:613	arg1	product					664:670	flavored dairy product	649:670	flavored dairy product	649:670	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	5	18	theme	dairy	609:613	arg1	yogurt					638:643	yogurt	638:643	yogurt	638:643	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	5	18	theme	dairy	609:613	arg1	products					615:622	three dairy products	603:622	three dairy products (milk powder, yogurt and flavored dairy product)	603:671	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	5	18	theme	dairy	609:613	arg1	powder					630:635	milk powder	625:635	milk powder	625:635	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	7	19	theme	freeze-drying	870:882	arg1	microcapsule					884:895	the freeze-drying microcapsule	866:895	the freeze-drying microcapsule	866:895	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	6	20	theme	dairy	676:680	arg1	product					682:688	A dairy product	674:688	A dairy product rich in Asta-C22:6 with high bioavailability	674:733	A dairy product rich in Asta-C22:6 with high bioavailability was designed by measuring quality characteristics, sensory evaluation and in vivo experiments.					
35396734	7	21	theme	best	1040:1043	arg1	quality					1045:1051	the best quality	1036:1051	the best quality	1036:1051	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	0	22	theme	acid	116:119	arg1	bioavailability					81:95	bioavailability	81:95	bioavailability	81:95	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid astaxanthin.					
35396734	0	22	theme	acid	116:119	arg1	characteristics					61:75	quality characteristics	53:75	quality characteristics	53:75	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid astaxanthin.					
35396734	0	23	from	matrix	39:44	arg1	Effects					0:6	Effects	0:6	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid	0:119	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid astaxanthin.					
35396734	13	24	theme	Chemical	1672:1679	arg1	Industry					1681:1688	Chemical Industry	1672:1688	Chemical Industry	1672:1688	© 2022 Society of Chemical Industry.					
35396734	11	25	theme	present	1430:1436	arg1	meaningful					1450:1459	meaningful	1450:1459	meaningful	1450:1459	The present research is meaningful for delivering unstable bioactive small molecules based on WPI and HPβ-CD.					
35396734	11	25	theme	present	1430:1436	arg1	research					1438:1445	The present research	1426:1445	The present research	1426:1445	The present research is meaningful for delivering unstable bioactive small molecules based on WPI and HPβ-CD.					
35396734	4	26	theme	protein	478:484	arg1	WPI					495:497	WPI	495:497	WPI	495:497	In this study, Asta-C22:6 microemulsion and microcapsule using whey protein isolate (WPI) and hydroxypropyl-β-cyclodextrin (HPβ-CD) as composite wall material were prepared.					
35396734	4	26	theme	protein	478:484	arg1	isolate					486:492	whey protein isolate	473:492	whey protein isolate (WPI)	473:498	In this study, Asta-C22:6 microemulsion and microcapsule using whey protein isolate (WPI) and hydroxypropyl-β-cyclodextrin (HPβ-CD) as composite wall material were prepared.					
35396734	6	27	theme	quality	761:767	arg1	characteristics					769:783	quality characteristics	761:783	quality characteristics	761:783	A dairy product rich in Asta-C22:6 with high bioavailability was designed by measuring quality characteristics, sensory evaluation and in vivo experiments.					
35396734	7	28	theme	microcapsule	1001:1012	arg1	yogurt					1025:1030	Asta-C22:6 microcapsule (1 g L-1 ) yogurt	990:1030	Asta-C22:6 microcapsule (1 g L-1 ) yogurt	990:1030	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	1	29	theme	docosahexaenoic	184:198	arg1	monoester					217:225	docosahexaenoic acid astaxanthin monoester	184:225	docosahexaenoic acid astaxanthin monoester (Asta-C22:6)	184:238	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	1	29	theme	docosahexaenoic	184:198	arg1	astaxanthin					164:174	free astaxanthin	159:174	free astaxanthin (Asta)	159:181	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	1	29	theme	docosahexaenoic	184:198	arg1	Asta-C22:6					228:237	Asta-C22:6	228:237	Asta-C22:6	228:237	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	11	30	theme	small	1495:1499	arg1	molecules					1501:1509	unstable bioactive small molecules	1476:1509	unstable bioactive small molecules based on WPI and HPβ-CD	1476:1533	The present research is meaningful for delivering unstable bioactive small molecules based on WPI and HPβ-CD.					
35396734	4	31	theme	composite	545:553	arg1	material					560:567	composite wall material	545:567	composite wall material	545:567	In this study, Asta-C22:6 microemulsion and microcapsule using whey protein isolate (WPI) and hydroxypropyl-β-cyclodextrin (HPβ-CD) as composite wall material were prepared.					
35396734	5	32	theme	milk	625:628	arg1	products					615:622	three dairy products	603:622	three dairy products (milk powder, yogurt and flavored dairy product)	603:671	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	5	32	theme	milk	625:628	arg1	powder					630:635	milk powder	625:635	milk powder	625:635	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	7	33	theme	encapsulation	910:922	arg1	efficiency					924:933	a higher encapsulation efficiency	901:933	a higher encapsulation efficiency (72.5%)	901:941	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	7	33	theme	encapsulation	910:922	arg1	%					940:940	72.5%	936:940	72.5%	936:940	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	7	34	contain	had	897:899	arg2	solubility					974:983	better solubility	967:983	better solubility	967:983	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	7	34	contain	had	897:899	arg2	efficiency					924:933	a higher encapsulation efficiency	901:933	a higher encapsulation efficiency (72.5%)	901:941	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	7	34	contain	had	897:899	arg2	content					950:956	water content	944:956	water content (4%)	944:961	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	7	34	contain	had	897:899	arg2	%					960:960	4%	959:960	4%	959:960	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	7	34	contain	had	897:899	arg2	%					940:940	72.5%	936:940	72.5%	936:940	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	7	34	contain	had	897:899	arg1	microcapsule					884:895	the freeze-drying microcapsule	866:895	the freeze-drying microcapsule	866:895	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	10	35	dep	CONCLUSION	1300:1309	arg1	prepared					1327:1334	prepared	1327:1334	prepared Asta-C22:6 microcapsule yogurt, which improved the stability and bioavailability of Asta	1327:1423	CONCLUSION We successfully prepared Asta-C22:6 microcapsule yogurt, which improved the stability and bioavailability of Asta.					
35396734	1	36	theme	acid	200:203	arg1	monoester					217:225	docosahexaenoic acid astaxanthin monoester	184:225	docosahexaenoic acid astaxanthin monoester (Asta-C22:6)	184:238	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	1	36	theme	acid	200:203	arg1	astaxanthin					164:174	free astaxanthin	159:174	free astaxanthin (Asta)	159:181	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	1	36	theme	acid	200:203	arg1	Asta-C22:6					228:237	Asta-C22:6	228:237	Asta-C22:6	228:237	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	6	37	dep	in	809:810	arg1	vivo					812:815	vivo	812:815	vivo	812:815	A dairy product rich in Asta-C22:6 with high bioavailability was designed by measuring quality characteristics, sensory evaluation and in vivo experiments.					
35396734	0	38	dep	characteristics	61:75	arg1	the					49:51	the	49:51	the	49:51	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid astaxanthin.					
35396734	1	39	theme	astaxanthin	205:215	arg1	monoester					217:225	docosahexaenoic acid astaxanthin monoester	184:225	docosahexaenoic acid astaxanthin monoester (Asta-C22:6)	184:238	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	1	39	theme	astaxanthin	205:215	arg1	astaxanthin					164:174	free astaxanthin	159:174	free astaxanthin (Asta)	159:181	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	1	39	theme	astaxanthin	205:215	arg1	Asta-C22:6					228:237	Asta-C22:6	228:237	Asta-C22:6	228:237	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	0	40	theme	microencapsulation	11:28	arg1	Effects					0:6	Effects	0:6	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid	0:119	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid astaxanthin.					
35396734	9	41	dep	increased	1240:1248	arg1	= 0.31 μg mL-1					1256:1269	Cmax = 0.31 μg mL-1	1251:1269	Cmax = 0.31 μg mL-1	1251:1269	After a single oral dose in mice, the bioavailability of Asta-C22:6 microcapsule in yogurt was significantly increased (Cmax = 0.31 μg mL-1 , AUC0-T = 3.20 h μg mL-1 ).					
35396734	9	41	dep	increased	1240:1248	arg1	= 3.20 h μg mL-1					1280:1295	AUC0-T = 3.20 h μg mL-1	1273:1295	AUC0-T = 3.20 h μg mL-1	1273:1295	After a single oral dose in mice, the bioavailability of Asta-C22:6 microcapsule in yogurt was significantly increased (Cmax = 0.31 μg mL-1 , AUC0-T = 3.20 h μg mL-1 ).					
35396734	9	42	theme	microcapsule	1199:1210	arg1	bioavailability					1169:1183	the bioavailability	1165:1183	the bioavailability of Asta-C22:6 microcapsule in yogurt	1165:1220	After a single oral dose in mice, the bioavailability of Asta-C22:6 microcapsule in yogurt was significantly increased (Cmax = 0.31 μg mL-1 , AUC0-T = 3.20 h μg mL-1 ).					
35396734	9	43	theme	oral	1146:1149	arg1	dose					1151:1154	a single oral dose	1137:1154	a single oral dose in mice	1137:1162	After a single oral dose in mice, the bioavailability of Asta-C22:6 microcapsule in yogurt was significantly increased (Cmax = 0.31 μg mL-1 , AUC0-T = 3.20 h μg mL-1 ).					
35396734	12	44	theme	products	1644:1651	arg1	development					1612:1622	the development	1608:1622	the development of functional dairy products	1608:1651	It provides an experimental basis for the application of Asta-C22:6 and the development of functional dairy products.					
35396734	12	44	theme	products	1644:1651	arg1	basis					1564:1568	an experimental basis	1548:1568	an experimental basis for the application of Asta-C22:6	1548:1602	It provides an experimental basis for the application of Asta-C22:6 and the development of functional dairy products.					
35396734	12	45	theme	functional	1627:1636	arg1	products					1644:1651	functional dairy products	1627:1651	functional dairy products	1627:1651	It provides an experimental basis for the application of Asta-C22:6 and the development of functional dairy products.					
35396734	4	46	theme	wall	555:558	arg1	material					560:567	composite wall material	545:567	composite wall material	545:567	In this study, Asta-C22:6 microemulsion and microcapsule using whey protein isolate (WPI) and hydroxypropyl-β-cyclodextrin (HPβ-CD) as composite wall material were prepared.					
35396734	4	47	theme	Asta-C22:6	425:434	arg1	microemulsion					436:448	Asta-C22:6 microemulsion	425:448	Asta-C22:6 microemulsion	425:448	In this study, Asta-C22:6 microemulsion and microcapsule using whey protein isolate (WPI) and hydroxypropyl-β-cyclodextrin (HPβ-CD) as composite wall material were prepared.					
35396734	11	48	theme	unstable	1476:1483	arg1	molecules					1501:1509	unstable bioactive small molecules	1476:1509	unstable bioactive small molecules based on WPI and HPβ-CD	1476:1533	The present research is meaningful for delivering unstable bioactive small molecules based on WPI and HPβ-CD.					
35396734	0	49	theme	dairy	33:37	arg1	matrix					39:44	dairy matrix	33:44	dairy matrix	33:44	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid astaxanthin.					
35396734	9	50	theme	Asta-C22:6	1188:1197	arg1	microcapsule					1199:1210	Asta-C22:6 microcapsule	1188:1210	Asta-C22:6 microcapsule	1188:1210	After a single oral dose in mice, the bioavailability of Asta-C22:6 microcapsule in yogurt was significantly increased (Cmax = 0.31 μg mL-1 , AUC0-T = 3.20 h μg mL-1 ).					
35396734	7	51	theme	better	967:972	arg1	solubility					974:983	better solubility	967:983	better solubility	967:983	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	6	52	with	Asta-C22:6	698:707	arg1	bioavailability					719:733	high bioavailability	714:733	high bioavailability	714:733	A dairy product rich in Asta-C22:6 with high bioavailability was designed by measuring quality characteristics, sensory evaluation and in vivo experiments.					
35396734	3	53	theme	water-soluble	379:391	arg1	system					402:407	a water-soluble delivery system	377:407	a water-soluble delivery system	377:407	Hence it is necessary to build a water-soluble delivery system.					
35396734	5	54	theme	flavored	649:656	arg1	product					664:670	flavored dairy product	649:670	flavored dairy product	649:670	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	5	54	theme	flavored	649:656	arg1	products					615:622	three dairy products	603:622	three dairy products (milk powder, yogurt and flavored dairy product)	603:671	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	8	55	theme	Asta-C22:6	1077:1086	arg1	yogurt					1101:1106	Asta-C22:6 microcapsule yogurt	1077:1106	Asta-C22:6 microcapsule yogurt	1077:1106	The bioavailability of Asta-C22:6 microcapsule yogurt was further evaluated.					
35396734	1	56	contain	has	240:242	arg2	bioavailability					265:279	bioavailability	265:279	bioavailability	265:279	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	1	56	contain	has	240:242	arg2	stability					251:259	higher stability	244:259	higher stability	244:259	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	1	56	contain	has	240:242	arg1	BACKGROUND					134:143	BACKGROUND	134:143	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6)	134:238	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	3	57	theme	delivery	393:400	arg1	system					402:407	a water-soluble delivery system	377:407	a water-soluble delivery system	377:407	Hence it is necessary to build a water-soluble delivery system.					
35396734	5	58	theme	dairy	658:662	arg1	product					664:670	flavored dairy product	649:670	flavored dairy product	649:670	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	5	58	theme	dairy	658:662	arg1	products					615:622	three dairy products	603:622	three dairy products (milk powder, yogurt and flavored dairy product)	603:671	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	2	59	theme	water	332:336	arg1	system					338:343	the water system	328:343	the water system	328:343	However, Asta-E is still unable to be used in the water system.					
35396734	11	60	theme	bioactive	1485:1493	arg1	molecules					1501:1509	unstable bioactive small molecules	1476:1509	unstable bioactive small molecules based on WPI and HPβ-CD	1476:1533	The present research is meaningful for delivering unstable bioactive small molecules based on WPI and HPβ-CD.					
35396734	6	61	theme	high	714:717	arg1	bioavailability					719:733	high bioavailability	714:733	high bioavailability	714:733	A dairy product rich in Asta-C22:6 with high bioavailability was designed by measuring quality characteristics, sensory evaluation and in vivo experiments.					
35396734	7	62	contain	had	1032:1034	arg2	quality					1045:1051	the best quality	1036:1051	the best quality	1036:1051	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	7	62	contain	had	1032:1034	arg1	yogurt					1025:1030	Asta-C22:6 microcapsule (1 g L-1 ) yogurt	990:1030	Asta-C22:6 microcapsule (1 g L-1 ) yogurt	990:1030	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	7	63	theme	spray	852:856	arg1	drying					858:863	spray drying	852:863	spray drying	852:863	RESULTS Compared with spray drying, the freeze-drying microcapsule had a higher encapsulation efficiency (72.5%), water content (4%) and better solubility, and Asta-C22:6 microcapsule (1 g L-1 ) yogurt had the best quality.					
35396734	5	64	dep	products	615:622	arg1	product					664:670	flavored dairy product	649:670	flavored dairy product	649:670	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	5	64	dep	products	615:622	arg1	yogurt					638:643	yogurt	638:643	yogurt	638:643	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	5	64	dep	products	615:622	arg1	products					615:622	three dairy products	603:622	three dairy products (milk powder, yogurt and flavored dairy product)	603:671	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	5	64	dep	products	615:622	arg1	powder					630:635	milk powder	625:635	milk powder	625:635	They were added to three dairy products (milk powder, yogurt and flavored dairy product).					
35396734	9	65	from	dose	1151:1154	arg1	mice					1159:1162	mice	1159:1162	mice	1159:1162	After a single oral dose in mice, the bioavailability of Asta-C22:6 microcapsule in yogurt was significantly increased (Cmax = 0.31 μg mL-1 , AUC0-T = 3.20 h μg mL-1 ).					
35396734	8	66	theme	microcapsule	1088:1099	arg1	yogurt					1101:1106	Asta-C22:6 microcapsule yogurt	1077:1106	Asta-C22:6 microcapsule yogurt	1077:1106	The bioavailability of Asta-C22:6 microcapsule yogurt was further evaluated.					
35396734	6	67	theme	in	809:810	arg1	experiments					817:827	in vivo experiments	809:827	in vivo experiments	809:827	A dairy product rich in Asta-C22:6 with high bioavailability was designed by measuring quality characteristics, sensory evaluation and in vivo experiments.					
35396734	10	68	theme	microcapsule	1347:1358	arg1	yogurt					1360:1365	Asta-C22:6 microcapsule yogurt	1336:1365	Asta-C22:6 microcapsule yogurt	1336:1365	CONCLUSION We successfully prepared Asta-C22:6 microcapsule yogurt, which improved the stability and bioavailability of Asta.					
35396734	1	69	theme	free	159:162	arg1	monoester					217:225	docosahexaenoic acid astaxanthin monoester	184:225	docosahexaenoic acid astaxanthin monoester (Asta-C22:6)	184:238	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	1	69	theme	free	159:162	arg1	astaxanthin					164:174	free astaxanthin	159:174	free astaxanthin (Asta)	159:181	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	1	69	theme	free	159:162	arg1	Asta					177:180	Asta	177:180	Asta	177:180	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	2	70	used	used	320:323	arg2	Asta-E					291:296	Asta-E	291:296	Asta-E	291:296	However, Asta-E is still unable to be used in the water system.					
35396734	1	71	theme	higher	244:249	arg1	stability					251:259	higher stability	244:259	higher stability	244:259	BACKGROUND Compared with free astaxanthin (Asta), docosahexaenoic acid astaxanthin monoester (Asta-C22:6) has higher stability and bioavailability.					
35396734	0	72	theme	quality	53:59	arg1	characteristics					61:75	quality characteristics	53:75	quality characteristics	53:75	Effects of microencapsulation in dairy matrix on the quality characteristics and bioavailability of docosahexaenoic acid astaxanthin.					
35396734	10	73	dep	stability	1387:1395	arg1	the					1383:1385	the	1383:1385	the	1383:1385	CONCLUSION We successfully prepared Asta-C22:6 microcapsule yogurt, which improved the stability and bioavailability of Asta.					
35396734	10	74	theme	Asta-C22:6	1336:1345	arg1	yogurt					1360:1365	Asta-C22:6 microcapsule yogurt	1336:1365	Asta-C22:6 microcapsule yogurt	1336:1365	CONCLUSION We successfully prepared Asta-C22:6 microcapsule yogurt, which improved the stability and bioavailability of Asta.					
35396734	6	75	from	rich	690:693	arg1	Asta-C22:6					698:707	Asta-C22:6	698:707	Asta-C22:6 with high bioavailability	698:733	A dairy product rich in Asta-C22:6 with high bioavailability was designed by measuring quality characteristics, sensory evaluation and in vivo experiments.					
35396734	12	76	theme	experimental	1551:1562	arg1	basis					1564:1568	an experimental basis	1548:1568	an experimental basis for the application of Asta-C22:6	1548:1602	It provides an experimental basis for the application of Asta-C22:6 and the development of functional dairy products.					
37217062	0	0	theme	chronic	114:120	arg1	healing					137:143	infected chronic diabetic wound healing	105:143	infected chronic diabetic wound healing	105:143	Hyaluronic acid-based injectable nanocomposite hydrogels with photo-thermal antibacterial properties for infected chronic diabetic wound healing.					
37217062	4	1	dep	characteristics	1058:1072	arg1	addition					1033:1040	addition	1033:1040	addition	1033:1040	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	5	2	from	formation	1514:1522	arg1	sites					1537:1541	the wound sites	1527:1541	the wound sites	1527:1541	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	1	3	theme	single	179:184	arg1	functionality					186:198	a single functionality	177:198	a single functionality	177:198	A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments.					
37217062	4	4	theme	vitro	1101:1105	arg1	adhesion					1113:1120	vitro, good adhesion	1101:1120	adhesion	1113:1120	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	1	5	theme	wounds	285:290	arg1	treatment					255:263	successful clinical treatment	235:263	successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments	235:343	A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments.					
37217062	0	6	theme	infected	105:112	arg1	healing					137:143	infected chronic diabetic wound healing	105:143	infected chronic diabetic wound healing	105:143	Hyaluronic acid-based injectable nanocomposite hydrogels with photo-thermal antibacterial properties for infected chronic diabetic wound healing.					
37217062	2	7	theme	clinical	411:418	arg1	treatment					420:428	improved clinical treatment	402:428	improved clinical treatment	402:428	A multifunctional hydrogel is thus highly desirable for improved clinical treatment.					
37217062	6	8	theme	wound	1688:1692	arg1	repair					1694:1699	infected diabetic wound repair	1670:1699	infected diabetic wound repair	1670:1699	Overall, the HA-based injectable composite hydrogels developed herein represent promising multifunctional wound dressings for infected diabetic wound repair.					
37217062	0	9	theme	wound	131:135	arg1	healing					137:143	infected chronic diabetic wound healing	105:143	infected chronic diabetic wound healing	105:143	Hyaluronic acid-based injectable nanocomposite hydrogels with photo-thermal antibacterial properties for infected chronic diabetic wound healing.					
37217062	3	10	theme	hexamethylene	771:783	arg1	guanidine					785:793	hexamethylene guanidine	771:793	hexamethylene guanidine	771:793	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	3	10	theme	hexamethylene	771:783	arg1	poly					766:769	poly	766:769	poly(hexamethylene guanidine) (PHMG)	766:801	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	4	11	theme	good	1108:1111	arg1	adhesion					1113:1120	vitro, good adhesion	1101:1120	adhesion	1113:1120	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	0	12	theme	diabetic	122:129	arg1	healing					137:143	infected chronic diabetic wound healing	105:143	infected chronic diabetic wound healing	105:143	Hyaluronic acid-based injectable nanocomposite hydrogels with photo-thermal antibacterial properties for infected chronic diabetic wound healing.					
37217062	5	13	theme	Wound	1152:1156	arg1	healing					1158:1164	Wound healing	1152:1164	Wound healing experiments in vivo	1152:1184	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	3	14	theme	electrostatic	639:651	arg1	interactions					653:664	an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions	571:664	an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs)	571:840	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	5	15	theme	wound	1531:1535	arg1	sites					1537:1541	the wound sites	1527:1541	the wound sites	1527:1541	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	3	16	theme	adhesive	588:595	arg1	interactions					653:664	an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions	571:664	an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs)	571:840	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	5	17	theme	granulation	1494:1504	arg1	tissues					1506:1512	granulation tissues	1494:1512	granulation tissues	1494:1512	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	5	18	theme	developed	1234:1242	arg1	hydrogels					1244:1252	the developed hydrogels	1230:1252	the developed hydrogels	1230:1252	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	5	19	theme	healing	1158:1164	arg1	experiments					1166:1176	Wound healing experiments	1152:1176	Wound healing experiments in vivo	1152:1184	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	4	20	theme	bacteria	904:911	arg1	bacteria					904:911	bacteria	904:911	bacteria (E. coli and S. aureus)	904:935	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	4	20	theme	bacteria	904:911	arg1	%					899:899	99.99 %	893:899	99.99 % of bacteria (E. coli and S. aureus)	893:935	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	5	21	theme	available	1282:1290	arg1	Tegaderm™					1302:1310	Tegaderm™	1302:1310	Tegaderm™	1302:1310	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	5	21	theme	available	1282:1290	arg1	dressing					1292:1299	a commercially available dressing	1267:1299	a commercially available dressing (Tegaderm™)	1267:1311	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	3	22	theme	addition	617:624	arg1	reaction					626:633	dynamic Michael addition reaction	601:633	dynamic Michael addition reaction	601:633	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	6	23	theme	injectable	1566:1575	arg1	hydrogels					1587:1595	the HA-based injectable composite hydrogels	1553:1595	the HA-based injectable composite hydrogels developed herein	1553:1612	Overall, the HA-based injectable composite hydrogels developed herein represent promising multifunctional wound dressings for infected diabetic wound repair.					
37217062	3	24	theme	nanocomposite	498:510	arg1	hydrogel					512:519	an injectable nanocomposite hydrogel	484:519	an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs)	484:840	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	4	25	theme	viscoelastic	1045:1056	arg1	characteristics					1058:1072	viscoelastic characteristics	1045:1072	viscoelastic characteristics	1045:1072	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	4	25	theme	viscoelastic	1045:1056	arg1	properties					1087:1096	degradation properties	1075:1096	degradation properties in vitro, good adhesion and self-adaptation capacity	1075:1149	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	1	26	with	dressing	163:170	arg1	functionality					186:198	a single functionality	177:198	a single functionality	177:198	A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments.					
37217062	6	27	theme	wound	1650:1654	arg1	dressings					1656:1664	promising multifunctional wound dressings	1624:1664	promising multifunctional wound dressings for infected diabetic wound repair	1624:1699	Overall, the HA-based injectable composite hydrogels developed herein represent promising multifunctional wound dressings for infected diabetic wound repair.					
37217062	3	28	theme	hydrogel	512:519	arg1	construction					468:479	construction	468:479	construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs)	468:840	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	0	29	theme	acid-based	11:20	arg1	nanocomposite					33:45	Hyaluronic acid-based injectable nanocomposite	0:45	Hyaluronic acid-based injectable nanocomposite	0:45	Hyaluronic acid-based injectable nanocomposite hydrogels with photo-thermal antibacterial properties for infected chronic diabetic wound healing.					
37217062	3	30	theme	black	808:812	arg1	BPs					837:839	BPs	837:839	BPs	837:839	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	3	30	theme	black	808:812	arg1	nanosheets					825:834	black phosphorus nanosheets	808:834	black phosphorus nanosheets (BPs)	808:840	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	3	31	theme	phosphorus	814:823	arg1	BPs					837:839	BPs	837:839	BPs	837:839	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	3	31	theme	phosphorus	814:823	arg1	nanosheets					825:834	black phosphorus nanosheets	808:834	black phosphorus nanosheets (BPs)	808:840	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	6	32	theme	multifunctional	1634:1648	arg1	dressings					1656:1664	promising multifunctional wound dressings	1624:1664	promising multifunctional wound dressings for infected diabetic wound repair	1624:1699	Overall, the HA-based injectable composite hydrogels developed herein represent promising multifunctional wound dressings for infected diabetic wound repair.					
37217062	0	33	theme	Hyaluronic	0:9	arg1	nanocomposite					33:45	Hyaluronic acid-based injectable nanocomposite	0:45	Hyaluronic acid-based injectable nanocomposite	0:45	Hyaluronic acid-based injectable nanocomposite hydrogels with photo-thermal antibacterial properties for infected chronic diabetic wound healing.					
37217062	1	34	theme	complicated	315:325	arg1	microenvironments					327:343	complicated microenvironments	315:343	complicated microenvironments	315:343	A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments.					
37217062	6	35	theme	composite	1577:1585	arg1	hydrogels					1587:1595	the HA-based injectable composite hydrogels	1553:1595	the HA-based injectable composite hydrogels developed herein	1553:1612	Overall, the HA-based injectable composite hydrogels developed herein represent promising multifunctional wound dressings for infected diabetic wound repair.					
37217062	3	36	theme	building	678:685	arg1	moieties					687:694	three building moieties	672:694	three building moieties	672:694	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	6	37	theme	promising	1624:1632	arg1	dressings					1656:1664	promising multifunctional wound dressings	1624:1664	promising multifunctional wound dressings for infected diabetic wound repair	1624:1699	Overall, the HA-based injectable composite hydrogels developed herein represent promising multifunctional wound dressings for infected diabetic wound repair.					
37217062	3	38	theme	injectable	487:496	arg1	hydrogel					512:519	an injectable nanocomposite hydrogel	484:519	an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs)	484:840	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	4	39	dep	bacteria	904:911	arg1	coli					917:920	E. coli	914:920	E. coli	914:920	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	4	39	dep	bacteria	904:911	arg1	aureus					929:934	S. aureus	926:934	S. aureus	926:934	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	4	40	from	properties	1087:1096	arg1	adhesion					1113:1120	vitro, good adhesion	1101:1120	adhesion	1113:1120	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	4	40	from	properties	1087:1096	arg1	capacity					1142:1149	self-adaptation capacity	1126:1149	self-adaptation capacity	1126:1149	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	4	41	theme	radical	958:964	arg1	capability					977:986	a free radical scavenging capability	951:986	a free radical scavenging capability >70 % as well as photo-thermal properties	951:1028	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	3	42	dep	catechol	703:710	arg1	i.e.					697:700	i.e.	697:700	i.e.	697:700	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	0	43	theme	injectable	22:31	arg1	nanocomposite					33:45	Hyaluronic acid-based injectable nanocomposite	0:45	Hyaluronic acid-based injectable nanocomposite	0:45	Hyaluronic acid-based injectable nanocomposite hydrogels with photo-thermal antibacterial properties for infected chronic diabetic wound healing.					
37217062	4	44	theme	degradation	1075:1085	arg1	characteristics					1058:1072	viscoelastic characteristics	1045:1072	viscoelastic characteristics	1045:1072	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	4	44	theme	degradation	1075:1085	arg1	properties					1087:1096	degradation properties	1075:1096	degradation properties in vitro, good adhesion and self-adaptation capacity	1075:1149	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	2	45	theme	multifunctional	348:362	arg1	hydrogel					364:371	A multifunctional hydrogel	346:371	A multifunctional hydrogel	346:371	A multifunctional hydrogel is thus highly desirable for improved clinical treatment.					
37217062	2	45	theme	multifunctional	348:362	arg1	desirable					388:396	desirable	388:396	desirable	388:396	A multifunctional hydrogel is thus highly desirable for improved clinical treatment.					
37217062	5	46	theme	wound	1379:1383	arg1	infection					1385:1393	wound infection	1379:1393	wound infection	1379:1393	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	3	47	theme	photo-thermal	543:555	arg1	properties					557:566	self-healing and photo-thermal properties	526:566	self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs)	526:840	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	4	48	theme	optimized	846:854	arg1	formulation					865:875	An optimized hydrogel formulation	843:875	An optimized hydrogel formulation	843:875	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	3	49	theme	antibacterial	574:586	arg1	interactions					653:664	an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions	571:664	an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs)	571:840	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	5	50	from	experiments	1166:1176	arg1	vivo					1181:1184	vivo	1181:1184	vivo	1181:1184	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	6	51	theme	HA-based	1557:1564	arg1	hydrogels					1587:1595	the HA-based injectable composite hydrogels	1553:1595	the HA-based injectable composite hydrogels developed herein	1553:1612	Overall, the HA-based injectable composite hydrogels developed herein represent promising multifunctional wound dressings for infected diabetic wound repair.					
37217062	1	52	theme	successful	235:244	arg1	treatment					255:263	successful clinical treatment	235:263	successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments	235:343	A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments.					
37217062	5	53	theme	better	1208:1213	arg1	performance					1215:1225	the better performance	1204:1225	the better performance of the developed hydrogels	1204:1252	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	4	54	theme	scavenging	966:975	arg1	capability					977:986	a free radical scavenging capability	951:986	a free radical scavenging capability >70 % as well as photo-thermal properties	951:1028	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	1	55	theme	clinical	246:253	arg1	treatment					255:263	successful clinical treatment	235:263	successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments	235:343	A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments.					
37217062	0	56	theme	antibacterial	76:88	arg1	properties					90:99	photo-thermal antibacterial properties	62:99	photo-thermal antibacterial properties for infected chronic diabetic wound healing	62:143	Hyaluronic acid-based injectable nanocomposite hydrogels with photo-thermal antibacterial properties for infected chronic diabetic wound healing.					
37217062	5	57	theme	hydrogels	1244:1252	arg1	performance					1215:1225	the better performance	1204:1225	the better performance of the developed hydrogels	1204:1252	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	2	58	theme	improved	402:409	arg1	treatment					420:428	improved clinical treatment	402:428	improved clinical treatment	402:428	A multifunctional hydrogel is thus highly desirable for improved clinical treatment.					
37217062	0	59	theme	photo-thermal	62:74	arg1	properties					90:99	photo-thermal antibacterial properties	62:99	photo-thermal antibacterial properties for infected chronic diabetic wound healing	62:143	Hyaluronic acid-based injectable nanocomposite hydrogels with photo-thermal antibacterial properties for infected chronic diabetic wound healing.					
37217062	1	60	theme	hydrogel	148:155	arg1	dressing					163:170	A hydrogel wound dressing	146:170	A hydrogel wound dressing with a single functionality	146:198	A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments.					
37217062	4	61	theme	self-adaptation	1126:1140	arg1	capacity					1142:1149	self-adaptation capacity	1126:1149	self-adaptation capacity	1126:1149	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	3	62	theme	thiol-modified	716:729	arg1	acid					742:745	thiol-modified hyaluronic acid	716:745	thiol-modified hyaluronic acid (HA-CA and HA-SH)	716:763	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	1	63	contain	possess	307:313	arg2	microenvironments					327:343	complicated microenvironments	315:343	complicated microenvironments	315:343	A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments.					
37217062	1	63	contain	possess	307:313	arg1	wounds					285:290	chronic diabetic wounds	268:290	chronic diabetic wounds that generally possess complicated microenvironments	268:343	A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments.					
37217062	1	64	theme	wound	157:161	arg1	dressing					163:170	A hydrogel wound dressing	146:170	A hydrogel wound dressing with a single functionality	146:198	A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments.					
37217062	5	65	theme	infected	1341:1348	arg1	wounds					1358:1363	infected chronic wounds	1341:1363	infected chronic wounds	1341:1363	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	3	66	dep	acid	742:745	arg1	HA-CA					748:752	HA-CA	748:752	HA-CA	748:752	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	3	66	dep	acid	742:745	arg1	HA-SH					758:762	HA-SH	758:762	HA-SH	758:762	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	3	67	with	hydrogel	512:519	arg1	properties					557:566	self-healing and photo-thermal properties	526:566	self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs)	526:840	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	6	68	theme	infected	1670:1677	arg1	repair					1694:1699	infected diabetic wound repair	1670:1699	infected diabetic wound repair	1670:1699	Overall, the HA-based injectable composite hydrogels developed herein represent promising multifunctional wound dressings for infected diabetic wound repair.					
37217062	3	69	theme	hyaluronic	731:740	arg1	acid					742:745	thiol-modified hyaluronic acid	716:745	thiol-modified hyaluronic acid (HA-CA and HA-SH)	716:763	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	5	70	theme	chronic	1350:1356	arg1	wounds					1358:1363	infected chronic wounds	1341:1363	infected chronic wounds	1341:1363	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	4	71	theme	free	953:956	arg1	capability					977:986	a free radical scavenging capability	951:986	a free radical scavenging capability >70 % as well as photo-thermal properties	951:1028	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	4	72	theme	photo-thermal	1005:1017	arg1	properties					1019:1028	photo-thermal properties	1005:1028	a free radical scavenging capability >70 % as well as photo-thermal properties	951:1028	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	3	73	theme	self-healing	526:537	arg1	properties					557:566	self-healing and photo-thermal properties	526:566	self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs)	526:840	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	5	74	theme	collagen	1432:1439	arg1	deposition					1441:1450	collagen deposition	1432:1450	collagen deposition	1432:1450	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	3	75	theme	dynamic	601:607	arg1	reaction					626:633	dynamic Michael addition reaction	601:633	dynamic Michael addition reaction	601:633	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	5	76	theme	wounds	1358:1363	arg1	healing					1330:1336	the healing	1326:1336	the healing of infected chronic wounds	1326:1363	Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites.					
37217062	1	77	theme	chronic	268:274	arg1	wounds					285:290	chronic diabetic wounds	268:290	chronic diabetic wounds that generally possess complicated microenvironments	268:343	A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments.					
37217062	4	78	theme	hydrogel	856:863	arg1	formulation					865:875	An optimized hydrogel formulation	843:875	An optimized hydrogel formulation	843:875	An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity.					
37217062	6	79	theme	diabetic	1679:1686	arg1	repair					1694:1699	infected diabetic wound repair	1670:1699	infected diabetic wound repair	1670:1699	Overall, the HA-based injectable composite hydrogels developed herein represent promising multifunctional wound dressings for infected diabetic wound repair.					
37217062	3	80	theme	Michael	609:615	arg1	reaction					626:633	dynamic Michael addition reaction	601:633	dynamic Michael addition reaction	601:633	For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs).					
37217062	1	81	theme	diabetic	276:283	arg1	wounds					285:290	chronic diabetic wounds	268:290	chronic diabetic wounds that generally possess complicated microenvironments	268:343	A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments.					
35121292	9	0	from	efficiencies	1910:1921	arg1	steps					1963:1967	the four recovery steps	1945:1967	the four recovery steps	1945:1967	After the adsorption process, the antibiotic-loaded adsorbent was regenerated by NaOH (0.01 mol L-1), and the reusability tests showed that the removal efficiencies of the antibiotics in the four recovery steps were still above 90%.					
35121292	0	1	from	wastewater	164:173	arg1	removal					121:127	removal	121:127	removal of antibiotics from pharmaceutical wastewater	121:173	The potential application of bio-based ceramic/organic xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater.					
35121292	7	2	theme	adsorption	1408:1417	arg1	process					1419:1425	the adsorption process	1404:1425	the adsorption process	1404:1425	Thermodynamic studies showed that the adsorption process was endothermic, spontaneous, and occurred by combination of physical and chemical mechanisms.					
35121292	8	3	theme	G	1613:1613	arg1	antibiotics					1615:1625	AMX, TC, CLX, and PEN G antibiotics	1591:1625	AMX, TC, CLX, and PEN G antibiotics	1591:1625	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	1	4	theme	bagasse	273:279	arg1	ash					281:283	sugarcane bagasse ash	263:283	sugarcane bagasse ash	263:283	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	4	theme	bagasse	273:279	arg1	sources					388:394	abundant, non-toxic, and renewable sources	353:394	abundant, non-toxic, and renewable sources	353:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	4	theme	bagasse	273:279	arg1	extract					334:340	pine cone-derived tannin extract	309:340	pine cone-derived tannin extract	309:340	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	4	theme	bagasse	273:279	arg1	alcohol					296:302	polyvinyl alcohol	286:302	polyvinyl alcohol	286:302	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	8	5	from	efficiency	1682:1691	arg1	effluents					1747:1755	three hospital effluents	1732:1755	three hospital effluents	1732:1755	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	8	5	from	efficiency	1682:1691	arg1	water					1702:1706	river water	1696:1706	river water	1696:1706	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	4	6	theme	%	1059:1059	arg1	limits					1072:1077	95% confidence limits	1057:1077	95% confidence limits	1057:1077	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	1	7	theme	ceramic/organic	188:202	arg1	BCO-xerogel					213:223	BCO-xerogel	213:223	BCO-xerogel	213:223	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	7	theme	ceramic/organic	188:202	arg1	xerogel					204:210	A bio-based ceramic/organic xerogel	176:210	A bio-based ceramic/organic xerogel (BCO-xerogel)	176:224	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	4	8	theme	conditions	1018:1027	arg1	interactions					1039:1050	their interactions	1033:1050	their interactions with 95% confidence limits	1033:1077	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	4	8	theme	conditions	1018:1027	arg1	significance					978:989	the significance	974:989	the significance of operating environmental conditions	974:1027	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	0	9	theme	adsorbent	107:115	arg1	green					101:105	A new green	95:105	the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater	76:173	The potential application of bio-based ceramic/organic xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater.					
35121292	2	10	theme	used	515:518	arg1	antibiotics					520:530	the most widely used antibiotics	499:530	the most widely used antibiotics	499:530	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	2	10	theme	used	515:518	arg1	amoxicillin					543:553	amoxicillin	543:553	amoxicillin (AMX)	543:559	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	2	10	theme	used	515:518	arg1	G					613:613	penicillin G	602:613	penicillin G (PEN G)	602:621	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	2	10	theme	used	515:518	arg1	tetracycline					562:573	tetracycline	562:573	tetracycline (TC)	562:578	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	2	10	theme	used	515:518	arg1	cefalexin					581:589	cefalexin	581:589	cefalexin (CLX)	581:595	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	3	11	theme	adsorbent	716:724	arg1	variables					696:704	conventional variables	683:704	conventional variables	683:704	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	11	theme	adsorbent	716:724	arg1	dosage					726:731	adsorbent dosage	716:731	adsorbent dosage	716:731	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	1	12	theme	renewable	378:386	arg1	ash					281:283	sugarcane bagasse ash	263:283	sugarcane bagasse ash	263:283	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	12	theme	renewable	378:386	arg1	sources					388:394	abundant, non-toxic, and renewable sources	353:394	abundant, non-toxic, and renewable sources	353:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	12	theme	renewable	378:386	arg1	extract					334:340	pine cone-derived tannin extract	309:340	pine cone-derived tannin extract	309:340	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	12	theme	renewable	378:386	arg1	alcohol					296:302	polyvinyl alcohol	286:302	polyvinyl alcohol	286:302	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	10	13	theme	economical	2051:2060	arg1	bio-adsorbent					2062:2074	green, efficient, and economical bio-adsorbent	2029:2074	green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters	2029:2156	This work explored the development of green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters.					
35121292	0	14	theme	antibiotics	132:142	arg1	removal					121:127	removal	121:127	removal of antibiotics from pharmaceutical wastewater	121:173	The potential application of bio-based ceramic/organic xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater.					
35121292	10	15	theme	antibiotics	2116:2126	arg1	removal					2105:2111	the removal	2101:2111	the removal of antibiotics from contaminated wastewaters	2101:2156	This work explored the development of green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters.					
35121292	2	16	theme	PEN	616:618	arg1	G					613:613	penicillin G	602:613	penicillin G (PEN G)	602:621	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	2	16	theme	PEN	616:618	arg1	G					620:620	PEN G	616:620	PEN G	616:620	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	0	17	theme	pharmaceutical	149:162	arg1	wastewater					164:173	pharmaceutical wastewater	149:173	pharmaceutical wastewater	149:173	The potential application of bio-based ceramic/organic xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater.					
35121292	8	18	theme	river	1696:1700	arg1	water					1702:1706	river water	1696:1706	river water	1696:1706	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	3	19	theme	contact	778:784	arg1	time					786:789	contact time	778:789	contact time	778:789	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	19	theme	contact	778:784	arg1	variables					696:704	conventional variables	683:704	conventional variables	683:704	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	10	20	used	utilized	2088:2095	arg2	bio-adsorbent					2062:2074	green, efficient, and economical bio-adsorbent	2029:2074	green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters	2029:2156	This work explored the development of green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters.					
35121292	1	21	theme	tannin	327:332	arg1	ash					281:283	sugarcane bagasse ash	263:283	sugarcane bagasse ash	263:283	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	21	theme	tannin	327:332	arg1	sources					388:394	abundant, non-toxic, and renewable sources	353:394	abundant, non-toxic, and renewable sources	353:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	21	theme	tannin	327:332	arg1	extract					334:340	pine cone-derived tannin extract	309:340	pine cone-derived tannin extract	309:340	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	21	theme	tannin	327:332	arg1	alcohol					296:302	polyvinyl alcohol	286:302	polyvinyl alcohol	286:302	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	2	22	theme	eliminate	472:480	arg1	residuals					623:631	the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals	468:631	the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water	468:655	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	9	23	theme	removal	1902:1908	arg1	efficiencies					1910:1921	the removal efficiencies	1898:1921	the removal efficiencies of the antibiotics in the four recovery steps	1898:1967	After the adsorption process, the antibiotic-loaded adsorbent was regenerated by NaOH (0.01 mol L-1), and the reusability tests showed that the removal efficiencies of the antibiotics in the four recovery steps were still above 90%.					
35121292	10	24	from	wastewaters	2146:2156	arg1	removal					2105:2111	the removal	2101:2111	the removal of antibiotics from contaminated wastewaters	2101:2156	This work explored the development of green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters.					
35121292	0	25	theme	plant	80:84	arg1	sources					86:92	the plant sources	76:92	the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater	76:173	The potential application of bio-based ceramic/organic xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater.					
35121292	7	26	theme	chemical	1501:1508	arg1	mechanisms					1510:1519	physical and chemical mechanisms	1488:1519	physical and chemical mechanisms	1488:1519	Thermodynamic studies showed that the adsorption process was endothermic, spontaneous, and occurred by combination of physical and chemical mechanisms.					
35121292	3	27	theme	solution	761:768	arg1	pH					770:771	solution pH	761:771	solution pH	761:771	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	27	theme	solution	761:768	arg1	variables					696:704	conventional variables	683:704	conventional variables	683:704	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	28	dep	variables	696:704	arg1	concentrations					745:758	antibiotic concentrations	734:758	antibiotic concentrations	734:758	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	28	dep	variables	696:704	arg1	time					786:789	contact time	778:789	contact time	778:789	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	28	dep	variables	696:704	arg1	variables					696:704	conventional variables	683:704	conventional variables	683:704	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	28	dep	variables	696:704	arg1	including					706:714	including	706:714	including	706:714	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	28	dep	variables	696:704	arg1	dosage					726:731	adsorbent dosage	716:731	adsorbent dosage	716:731	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	28	dep	variables	696:704	arg1	pH					770:771	solution pH	761:771	solution pH	761:771	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	4	29	with	significance	978:989	arg1	limits					1072:1077	95% confidence limits	1057:1077	95% confidence limits	1057:1077	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	7	30	theme	physical	1488:1495	arg1	mechanisms					1510:1519	physical and chemical mechanisms	1488:1519	physical and chemical mechanisms	1488:1519	Thermodynamic studies showed that the adsorption process was endothermic, spontaneous, and occurred by combination of physical and chemical mechanisms.					
35121292	1	31	theme	cone-derived	314:325	arg1	ash					281:283	sugarcane bagasse ash	263:283	sugarcane bagasse ash	263:283	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	31	theme	cone-derived	314:325	arg1	sources					388:394	abundant, non-toxic, and renewable sources	353:394	abundant, non-toxic, and renewable sources	353:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	31	theme	cone-derived	314:325	arg1	extract					334:340	pine cone-derived tannin extract	309:340	pine cone-derived tannin extract	309:340	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	31	theme	cone-derived	314:325	arg1	alcohol					296:302	polyvinyl alcohol	286:302	polyvinyl alcohol	286:302	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	0	32	theme	new	97:99	arg1	green					101:105	A new green	95:105	the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater	76:173	The potential application of bio-based ceramic/organic xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater.					
35121292	0	33	theme	ceramic/organic	39:53	arg1	application					14:24	The potential application	0:24	The potential application of bio-based ceramic/organic	0:53	The potential application of bio-based ceramic/organic xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater.					
35121292	8	34	theme	hospital	1738:1745	arg1	effluents					1747:1755	three hospital effluents	1732:1755	three hospital effluents	1732:1755	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	3	35	theme	composite	829:837	arg1	CCD					847:849	CCD	847:849	CCD	847:849	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	35	theme	composite	829:837	arg1	design					839:844	central composite design	821:844	central composite design (CCD)	821:850	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	5	36	theme	experimental	1112:1123	arg1	98.78 ± 3.25					1179:1190	98.78 ± 3.25	1179:1190	98.78 ± 3.25	1179:1190	Under optimized conditions, the experimental removal efficiencies for AMX, TC, CLX, and PEN G were 98.78 ± 3.25, 99.12 ± 2.52, 98.02 ± 1.98, and 98.42 ± 2.19, respectively.					
35121292	5	36	theme	experimental	1112:1123	arg1	efficiencies					1133:1144	the experimental removal efficiencies	1108:1144	the experimental removal efficiencies for AMX, TC, CLX, and PEN G	1108:1172	Under optimized conditions, the experimental removal efficiencies for AMX, TC, CLX, and PEN G were 98.78 ± 3.25, 99.12 ± 2.52, 98.02 ± 1.98, and 98.42 ± 2.19, respectively.					
35121292	10	37	theme	contaminated	2133:2144	arg1	wastewaters					2146:2156	contaminated wastewaters	2133:2156	contaminated wastewaters	2133:2156	This work explored the development of green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters.					
35121292	1	38	theme	ash	281:283	arg1	combination					248:258	the combination	244:258	the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources	244:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	3	39	theme	response	858:865	arg1	RSM					888:890	RSM	888:890	RSM	888:890	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	39	theme	response	858:865	arg1	methodology					875:885	response surface methodology	858:885	response surface methodology (RSM)	858:891	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	4	40	theme	statistical	941:951	arg1	Analysis					894:901	Analysis	894:901	Analysis of variance (ANOVA)	894:921	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	4	40	theme	statistical	941:951	arg1	formula					953:959	a statistical formula	939:959	a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits	939:1077	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	9	41	theme	recovery	1954:1961	arg1	steps					1963:1967	the four recovery steps	1945:1967	the four recovery steps	1945:1967	After the adsorption process, the antibiotic-loaded adsorbent was regenerated by NaOH (0.01 mol L-1), and the reusability tests showed that the removal efficiencies of the antibiotics in the four recovery steps were still above 90%.					
35121292	1	42	theme	polyvinyl	286:294	arg1	ash					281:283	sugarcane bagasse ash	263:283	sugarcane bagasse ash	263:283	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	42	theme	polyvinyl	286:294	arg1	alcohol					296:302	polyvinyl alcohol	286:302	polyvinyl alcohol	286:302	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	42	theme	polyvinyl	286:294	arg1	sources					388:394	abundant, non-toxic, and renewable sources	353:394	abundant, non-toxic, and renewable sources	353:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	42	theme	polyvinyl	286:294	arg1	extract					334:340	pine cone-derived tannin extract	309:340	pine cone-derived tannin extract	309:340	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	0	43	theme	bio-based	29:37	arg1	ceramic/organic					39:53	bio-based ceramic/organic	29:53	bio-based ceramic/organic	29:53	The potential application of bio-based ceramic/organic xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater.					
35121292	0	44	dep	xerogel	55:61	arg1	derived					63:69	derived	63:69	xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater	55:173	The potential application of bio-based ceramic/organic xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater.					
35121292	1	45	link	cone-derived	314:325	arg1	ash					281:283	sugarcane bagasse ash	263:283	sugarcane bagasse ash	263:283	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	45	link	cone-derived	314:325	arg1	sources					388:394	abundant, non-toxic, and renewable sources	353:394	abundant, non-toxic, and renewable sources	353:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	45	link	cone-derived	314:325	arg1	extract					334:340	pine cone-derived tannin extract	309:340	pine cone-derived tannin extract	309:340	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	45	link	cone-derived	314:325	arg1	alcohol					296:302	polyvinyl alcohol	286:302	polyvinyl alcohol	286:302	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	46	theme	pine	309:312	arg1	ash					281:283	sugarcane bagasse ash	263:283	sugarcane bagasse ash	263:283	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	46	theme	pine	309:312	arg1	sources					388:394	abundant, non-toxic, and renewable sources	353:394	abundant, non-toxic, and renewable sources	353:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	46	theme	pine	309:312	arg1	extract					334:340	pine cone-derived tannin extract	309:340	pine cone-derived tannin extract	309:340	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	46	theme	pine	309:312	arg1	alcohol					296:302	polyvinyl alcohol	286:302	polyvinyl alcohol	286:302	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	9	47	theme	adsorption	1768:1777	arg1	process					1779:1785	the adsorption process	1764:1785	the adsorption process	1764:1785	After the adsorption process, the antibiotic-loaded adsorbent was regenerated by NaOH (0.01 mol L-1), and the reusability tests showed that the removal efficiencies of the antibiotics in the four recovery steps were still above 90%.					
35121292	9	48	from	antibiotics	1930:1940	arg1	steps					1963:1967	the four recovery steps	1945:1967	the four recovery steps	1945:1967	After the adsorption process, the antibiotic-loaded adsorbent was regenerated by NaOH (0.01 mol L-1), and the reusability tests showed that the removal efficiencies of the antibiotics in the four recovery steps were still above 90%.					
35121292	8	49	dep	efficiency	1682:1691	arg1	%					1669:1669	%	1669:1669	%	1669:1669	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	4	50	theme	variance	906:913	arg1	Analysis					894:901	Analysis	894:901	Analysis of variance (ANOVA)	894:921	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	4	50	theme	variance	906:913	arg1	formula					953:959	a statistical formula	939:959	a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits	939:1077	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	5	51	theme	removal	1125:1131	arg1	98.78 ± 3.25					1179:1190	98.78 ± 3.25	1179:1190	98.78 ± 3.25	1179:1190	Under optimized conditions, the experimental removal efficiencies for AMX, TC, CLX, and PEN G were 98.78 ± 3.25, 99.12 ± 2.52, 98.02 ± 1.98, and 98.42 ± 2.19, respectively.					
35121292	5	51	theme	removal	1125:1131	arg1	efficiencies					1133:1144	the experimental removal efficiencies	1108:1144	the experimental removal efficiencies for AMX, TC, CLX, and PEN G	1108:1172	Under optimized conditions, the experimental removal efficiencies for AMX, TC, CLX, and PEN G were 98.78 ± 3.25, 99.12 ± 2.52, 98.02 ± 1.98, and 98.42 ± 2.19, respectively.					
35121292	8	52	dep	%	1669:1669	arg1	97.4-98.6					1660:1668	97.4-98.6	1660:1668	97.4-98.6	1660:1668	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	8	53	theme	BCO-xerogel	1554:1564	arg1	ability					1543:1549	the ability	1539:1549	the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics	1539:1625	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	1	54	theme	sugarcane	263:271	arg1	ash					281:283	sugarcane bagasse ash	263:283	sugarcane bagasse ash	263:283	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	54	theme	sugarcane	263:271	arg1	sources					388:394	abundant, non-toxic, and renewable sources	353:394	abundant, non-toxic, and renewable sources	353:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	54	theme	sugarcane	263:271	arg1	extract					334:340	pine cone-derived tannin extract	309:340	pine cone-derived tannin extract	309:340	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	54	theme	sugarcane	263:271	arg1	alcohol					296:302	polyvinyl alcohol	286:302	polyvinyl alcohol	286:302	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	4	55	theme	95	1057:1058	arg1	%					1059:1059	%	1059:1059	%	1059:1059	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	8	56	theme	adsorptive	1569:1578	arg1	removal					1580:1586	adsorptive removal	1569:1586	adsorptive removal of AMX, TC, CLX, and PEN G antibiotics	1569:1625	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	2	57	used	used	429:432	arg2	BCO-xerogel					413:423	The as-prepared BCO-xerogel	397:423	The as-prepared BCO-xerogel	397:423	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	2	57	used	used	429:432	arg2	adsorbent					454:462	adsorbent	454:462	adsorbent	454:462	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	8	58	theme	PEN	1609:1611	arg1	G					1613:1613	PEN G	1609:1613	PEN G	1609:1613	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	7	59	theme	Thermodynamic	1370:1382	arg1	studies					1384:1390	Thermodynamic studies	1370:1390	Thermodynamic studies	1370:1390	Thermodynamic studies showed that the adsorption process was endothermic, spontaneous, and occurred by combination of physical and chemical mechanisms.					
35121292	4	60	theme	confidence	1061:1070	arg1	limits					1072:1077	95% confidence limits	1057:1077	95% confidence limits	1057:1077	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	8	61	theme	antibiotics	1615:1625	arg1	removal					1580:1586	adsorptive removal	1569:1586	adsorptive removal of AMX, TC, CLX, and PEN G antibiotics	1569:1625	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	1	62	theme	bio-based	178:186	arg1	BCO-xerogel					213:223	BCO-xerogel	213:223	BCO-xerogel	213:223	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	62	theme	bio-based	178:186	arg1	xerogel					204:210	A bio-based ceramic/organic xerogel	176:210	A bio-based ceramic/organic xerogel (BCO-xerogel)	176:224	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	10	63	theme	efficient	2036:2044	arg1	bio-adsorbent					2062:2074	green, efficient, and economical bio-adsorbent	2029:2074	green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters	2029:2156	This work explored the development of green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters.					
35121292	3	64	theme	conventional	683:694	arg1	pH					770:771	solution pH	761:771	solution pH	761:771	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	64	theme	conventional	683:694	arg1	time					786:789	contact time	778:789	contact time	778:789	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	64	theme	conventional	683:694	arg1	variables					696:704	conventional variables	683:704	conventional variables	683:704	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	64	theme	conventional	683:694	arg1	dosage					726:731	adsorbent dosage	716:731	adsorbent dosage	716:731	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	8	65	theme	real	1630:1633	arg1	wastewaters					1635:1645	real wastewaters	1630:1645	real wastewaters	1630:1645	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	2	66	theme	antibiotics	520:530	arg1	types					490:494	four types	485:494	four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G)	485:621	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	10	67	theme	green	2029:2033	arg1	bio-adsorbent					2062:2074	green, efficient, and economical bio-adsorbent	2029:2074	green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters	2029:2156	This work explored the development of green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters.					
35121292	3	68	theme	simultaneous	662:673	arg1	effects					675:681	The simultaneous effects	658:681	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time	658:789	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	10	69	theme	bio-adsorbent	2062:2074	arg1	development					2014:2024	the development	2010:2024	the development of green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters	2010:2156	This work explored the development of green, efficient, and economical bio-adsorbent that can be utilized for the removal of antibiotics from contaminated wastewaters.					
35121292	2	70	from	water	651:655	arg1	residuals					623:631	the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals	468:631	the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water	468:655	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	9	71	theme	reusability	1868:1878	arg1	tests					1880:1884	the reusability tests	1864:1884	the reusability tests	1864:1884	After the adsorption process, the antibiotic-loaded adsorbent was regenerated by NaOH (0.01 mol L-1), and the reusability tests showed that the removal efficiencies of the antibiotics in the four recovery steps were still above 90%.					
35121292	8	72	from	%	1727:1727	arg1	effluents					1747:1755	three hospital effluents	1732:1755	three hospital effluents	1732:1755	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	8	72	from	%	1727:1727	arg1	water					1702:1706	river water	1696:1706	river water	1696:1706	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	8	73	theme	adsorption	1671:1680	arg1	efficiency					1682:1691	about 97.4-98.6% adsorption efficiency	1654:1691	about 97.4-98.6% adsorption efficiency in river water	1654:1706	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	7	74	theme	mechanisms	1510:1519	arg1	combination					1473:1483	combination	1473:1483	combination of physical and chemical mechanisms	1473:1519	Thermodynamic studies showed that the adsorption process was endothermic, spontaneous, and occurred by combination of physical and chemical mechanisms.					
35121292	2	75	theme	contaminated	638:649	arg1	water					651:655	contaminated water	638:655	contaminated water	638:655	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	3	76	theme	antibiotic	734:743	arg1	concentrations					745:758	antibiotic concentrations	734:758	antibiotic concentrations	734:758	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	1	77	theme	extract	334:340	arg1	combination					248:258	the combination	244:258	the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources	244:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	3	78	theme	central	821:827	arg1	CCD					847:849	CCD	847:849	CCD	847:849	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	78	theme	central	821:827	arg1	design					839:844	central composite design	821:844	central composite design (CCD)	821:850	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	5	79	theme	PEN	1168:1170	arg1	G					1172:1172	PEN G	1168:1172	PEN G	1168:1172	Under optimized conditions, the experimental removal efficiencies for AMX, TC, CLX, and PEN G were 98.78 ± 3.25, 99.12 ± 2.52, 98.02 ± 1.98, and 98.42 ± 2.19, respectively.					
35121292	6	80	theme	adsorption	1257:1266	arg1	isotherms					1268:1276	The adsorption isotherms	1253:1276	The adsorption isotherms	1253:1276	The adsorption isotherms and kinetics were better fitted with Langmuir and pseudo-second-order models, respectively.					
35121292	1	81	theme	abundant	353:360	arg1	ash					281:283	sugarcane bagasse ash	263:283	sugarcane bagasse ash	263:283	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	81	theme	abundant	353:360	arg1	sources					388:394	abundant, non-toxic, and renewable sources	353:394	abundant, non-toxic, and renewable sources	353:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	81	theme	abundant	353:360	arg1	extract					334:340	pine cone-derived tannin extract	309:340	pine cone-derived tannin extract	309:340	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	81	theme	abundant	353:360	arg1	alcohol					296:302	polyvinyl alcohol	286:302	polyvinyl alcohol	286:302	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	4	82	theme	environmental	1004:1016	arg1	conditions					1018:1027	operating environmental conditions	994:1027	operating environmental conditions	994:1027	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	9	83	from	steps	1963:1967	arg1	efficiencies					1910:1921	the removal efficiencies	1898:1921	the removal efficiencies of the antibiotics in the four recovery steps	1898:1967	After the adsorption process, the antibiotic-loaded adsorbent was regenerated by NaOH (0.01 mol L-1), and the reusability tests showed that the removal efficiencies of the antibiotics in the four recovery steps were still above 90%.					
35121292	0	84	theme	potential	4:12	arg1	application					14:24	The potential application	0:24	The potential application of bio-based ceramic/organic	0:53	The potential application of bio-based ceramic/organic xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater.					
35121292	1	85	theme	non-toxic	363:371	arg1	ash					281:283	sugarcane bagasse ash	263:283	sugarcane bagasse ash	263:283	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	85	theme	non-toxic	363:371	arg1	sources					388:394	abundant, non-toxic, and renewable sources	353:394	abundant, non-toxic, and renewable sources	353:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	85	theme	non-toxic	363:371	arg1	extract					334:340	pine cone-derived tannin extract	309:340	pine cone-derived tannin extract	309:340	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	1	85	theme	non-toxic	363:371	arg1	alcohol					296:302	polyvinyl alcohol	286:302	polyvinyl alcohol	286:302	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	0	86	dep	sources	86:92	arg1	green					101:105	A new green	95:105	the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater	76:173	The potential application of bio-based ceramic/organic xerogel derived from the plant sources: A new green adsorbent for removal of antibiotics from pharmaceutical wastewater.					
35121292	2	87	theme	penicillin	602:611	arg1	G					613:613	penicillin G	602:613	penicillin G (PEN G)	602:621	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	2	87	theme	penicillin	602:611	arg1	G					620:620	PEN G	616:620	PEN G	616:620	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	9	88	theme	antibiotics	1930:1940	arg1	efficiencies					1910:1921	the removal efficiencies	1898:1921	the removal efficiencies of the antibiotics in the four recovery steps	1898:1967	After the adsorption process, the antibiotic-loaded adsorbent was regenerated by NaOH (0.01 mol L-1), and the reusability tests showed that the removal efficiencies of the antibiotics in the four recovery steps were still above 90%.					
35121292	3	89	theme	surface	867:873	arg1	RSM					888:890	RSM	888:890	RSM	888:890	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	3	89	theme	surface	867:873	arg1	methodology					875:885	response surface methodology	858:885	response surface methodology (RSM)	858:891	The simultaneous effects conventional variables including adsorbent dosage, antibiotic concentrations, solution pH, and contact time were studied and optimized by central composite design (CCD) under response surface methodology (RSM).					
35121292	2	90	theme	as-prepared	401:411	arg1	adsorbent					454:462	adsorbent	454:462	adsorbent	454:462	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	2	90	theme	as-prepared	401:411	arg1	BCO-xerogel					413:423	The as-prepared BCO-xerogel	397:423	The as-prepared BCO-xerogel	397:423	The as-prepared BCO-xerogel was used as a low-cost green adsorbent for the eliminate of four types of the most widely used antibiotics, including amoxicillin (AMX), tetracycline (TC), cefalexin (CLX), and penicillin G (PEN G) residuals from contaminated water.					
35121292	6	91	theme	pseudo-second-order	1328:1346	arg1	models					1348:1353	pseudo-second-order models	1328:1353	pseudo-second-order models	1328:1353	The adsorption isotherms and kinetics were better fitted with Langmuir and pseudo-second-order models, respectively.					
35121292	1	92	theme	alcohol	296:302	arg1	combination					248:258	the combination	244:258	the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources	244:394	A bio-based ceramic/organic xerogel (BCO-xerogel) was obtained from the combination of sugarcane bagasse ash, polyvinyl alcohol, and pine cone-derived tannin extract, which are abundant, non-toxic, and renewable sources.					
35121292	4	93	with	interactions	1039:1050	arg1	limits					1072:1077	95% confidence limits	1057:1077	95% confidence limits	1057:1077	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	8	94	theme	AMX	1591:1593	arg1	antibiotics					1615:1625	AMX, TC, CLX, and PEN G antibiotics	1591:1625	AMX, TC, CLX, and PEN G antibiotics	1591:1625	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	5	95	theme	optimized	1086:1094	arg1	conditions					1096:1105	optimized conditions	1086:1105	optimized conditions	1086:1105	Under optimized conditions, the experimental removal efficiencies for AMX, TC, CLX, and PEN G were 98.78 ± 3.25, 99.12 ± 2.52, 98.02 ± 1.98, and 98.42 ± 2.19, respectively.					
35121292	8	96	theme	TC	1596:1597	arg1	antibiotics					1615:1625	AMX, TC, CLX, and PEN G antibiotics	1591:1625	AMX, TC, CLX, and PEN G antibiotics	1591:1625	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
35121292	4	97	theme	operating	994:1002	arg1	conditions					1018:1027	operating environmental conditions	994:1027	operating environmental conditions	994:1027	Analysis of variance (ANOVA) was employed as a statistical formula to determine the significance of operating environmental conditions and their interactions with 95% confidence limits.					
35121292	8	98	theme	CLX	1600:1602	arg1	antibiotics					1615:1625	AMX, TC, CLX, and PEN G antibiotics	1591:1625	AMX, TC, CLX, and PEN G antibiotics	1591:1625	Also, evaluating the ability of BCO-xerogel to adsorptive removal of AMX, TC, CLX, and PEN G antibiotics in real wastewaters showed about 97.4-98.6% adsorption efficiency in river water and about 67.1-71.3% in three hospital effluents.					
36657855	6	0	theme	considerable	1155:1166	arg1	synergism					1168:1176	a considerable synergism	1153:1176	a considerable synergism in the antituberculosis activity between TCNPs and graphene nanosheets	1153:1247	This reveals a considerable synergism in the antituberculosis activity between TCNPs and graphene nanosheets.					
36657855	7	1	theme	cell	1353:1356	arg1	viability					1358:1366	cell viability	1353:1366	cell viability of 100%	1353:1374	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	4	2	theme	developed	699:707	arg1	materials					709:717	these developed materials	693:717	these developed materials	693:717	The inhibitory activity of these developed materials was examined against the growth of three different M. tuberculosis pathogens and in a comparison with the isoniazid drug as a standard anti-tuberculosis drug.					
36657855	1	3	theme	IPNs	259:262	arg1	nanoparticles					264:276	TMC-CMS IPNs nanoparticles	251:276	TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach	251:342	Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach.					
36657855	0	4	theme	M.	83:84	arg1	tuberculosis					86:97	targeting M. tuberculosis	73:97	targeting M. tuberculosis	73:97	Green engineering of TMC-CMS nanoparticles decorated graphene sheets for targeting M. tuberculosis.					
36657855	5	5	theme	bare	1046:1049	arg1	TCNPs					1051:1055	the bare TCNPs	1042:1055	the bare TCNPs (7.81, 31.25, >125 μg/mL)	1042:1081	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	7	6	theme	concentration	1385:1397	arg1	range					1399:1403	the concentration range	1381:1403	the concentration range of 0.98-7.81 μg/mL	1381:1422	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	6	7	theme	antituberculosis	1185:1200	arg1	activity					1202:1209	the antituberculosis activity	1181:1209	the antituberculosis activity	1181:1209	This reveals a considerable synergism in the antituberculosis activity between TCNPs and graphene nanosheets.					
36657855	7	8	theme	%	1374:1374	arg1	viability					1358:1366	cell viability	1353:1366	cell viability of 100%	1353:1374	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	3	9	from	formation	516:524	arg1	surfaces					569:576	graphene surfaces	560:576	graphene surfaces	560:576	TEM data has proved the formation of uniformly distributed TCNPs on graphene surfaces with a small particle size of ~22 nm compared with that of pure nanoparticles (~30 nm).					
36657855	4	10	theme	M.	770:771	arg1	pathogens					786:794	three different M. tuberculosis pathogens	754:794	three different M. tuberculosis pathogens	754:794	The inhibitory activity of these developed materials was examined against the growth of three different M. tuberculosis pathogens and in a comparison with the isoniazid drug as a standard anti-tuberculosis drug.					
36657855	6	11	from	synergism	1168:1176	arg1	activity					1202:1209	the antituberculosis activity	1181:1209	the antituberculosis activity	1181:1209	This reveals a considerable synergism in the antituberculosis activity between TCNPs and graphene nanosheets.					
36657855	5	12	dep	drug	1101:1104	arg1	0 μg/mL					1116:1122	0 μg/mL	1116:1122	0 μg/mL	1116:1122	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	5	12	dep	drug	1101:1104	arg1	0.24					1107:1110	0.24	1107:1110	0.24	1107:1110	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	4	13	theme	different	760:768	arg1	pathogens					786:794	three different M. tuberculosis pathogens	754:794	three different M. tuberculosis pathogens	754:794	The inhibitory activity of these developed materials was examined against the growth of three different M. tuberculosis pathogens and in a comparison with the isoniazid drug as a standard anti-tuberculosis drug.					
36657855	3	14	theme	graphene	560:567	arg1	surfaces					569:576	graphene surfaces	560:576	graphene surfaces	560:576	TEM data has proved the formation of uniformly distributed TCNPs on graphene surfaces with a small particle size of ~22 nm compared with that of pure nanoparticles (~30 nm).					
36657855	1	15	theme	one-pot	288:294	arg1	approach					335:342	one-pot, green, cost-effective ultrasonication approach	288:342	one-pot, green, cost-effective ultrasonication approach	288:342	Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach.					
36657855	5	16	theme	MIC	911:913	arg1	values					915:920	MIC values	911:920	MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens	911:1028	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	3	17	theme	distributed	539:549	arg1	TCNPs					551:555	uniformly distributed TCNPs	529:555	uniformly distributed TCNPs	529:555	TEM data has proved the formation of uniformly distributed TCNPs on graphene surfaces with a small particle size of ~22 nm compared with that of pure nanoparticles (~30 nm).					
36657855	4	18	theme	inhibitory	670:679	arg1	activity					681:688	The inhibitory activity	666:688	The inhibitory activity of these developed materials	666:717	The inhibitory activity of these developed materials was examined against the growth of three different M. tuberculosis pathogens and in a comparison with the isoniazid drug as a standard anti-tuberculosis drug.					
36657855	1	19	theme	antibacterial	161:173	arg1	composite					175:183	antibacterial composite	161:183	antibacterial composite	161:183	Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach.					
36657855	4	20	with	comparison	805:814	arg1	drug					835:838	the isoniazid drug	821:838	the isoniazid drug as a standard anti-tuberculosis drug	821:875	The inhibitory activity of these developed materials was examined against the growth of three different M. tuberculosis pathogens and in a comparison with the isoniazid drug as a standard anti-tuberculosis drug.					
36657855	3	21	theme	TCNPs	551:555	arg1	formation					516:524	the formation	512:524	the formation of uniformly distributed TCNPs on graphene surfaces	512:576	TEM data has proved the formation of uniformly distributed TCNPs on graphene surfaces with a small particle size of ~22 nm compared with that of pure nanoparticles (~30 nm).					
36657855	1	22	theme	composite	175:183	arg1	type					153:156	a new type	147:156	a new type of antibacterial composite	147:183	Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach.					
36657855	7	23	theme	lung	1308:1311	arg1	WI38					1325:1328	WI38	1325:1328	WI38	1325:1328	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	7	23	theme	lung	1308:1311	arg1	lines					1318:1322	normal lung cell lines	1301:1322	normal lung cell lines (WI38)	1301:1329	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	5	24	theme	31.25	1064:1068	arg1	>125 μg/mL					1071:1080	>125 μg/mL	1071:1080	>125 μg/mL	1071:1080	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	5	24	theme	31.25	1064:1068	arg1	7.81					1058:1061	7.81	1058:1061	7.81	1058:1061	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	4	25	theme	anti-tuberculosis	854:870	arg1	drug					872:875	a standard anti-tuberculosis drug	843:875	a standard anti-tuberculosis drug	843:875	The inhibitory activity of these developed materials was examined against the growth of three different M. tuberculosis pathogens and in a comparison with the isoniazid drug as a standard anti-tuberculosis drug.					
36657855	2	26	theme	analytical	426:435	arg1	techniques					437:446	various analytical techniques	418:446	various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM	418:489	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	2	26	theme	analytical	426:435	arg1	UV/vis					470:475	UV/vis	470:475	UV/vis	470:475	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	2	26	theme	analytical	426:435	arg1	SEM					478:480	SEM	478:480	SEM	478:480	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	2	26	theme	analytical	426:435	arg1	1H-NMR					456:461	1H-NMR	456:461	1H-NMR	456:461	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	2	26	theme	analytical	426:435	arg1	FTIR					464:467	FTIR	464:467	FTIR	464:467	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	2	26	theme	analytical	426:435	arg1	TEM					487:489	TEM	487:489	TEM	487:489	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	0	27	theme	Green	0:4	arg1	engineering					6:16	Green engineering	0:16	Green engineering of TMC-CMS nanoparticles	0:41	Green engineering of TMC-CMS nanoparticles decorated graphene sheets for targeting M. tuberculosis.					
36657855	4	28	theme	standard	845:852	arg1	drug					872:875	a standard anti-tuberculosis drug	843:875	a standard anti-tuberculosis drug	843:875	The inhibitory activity of these developed materials was examined against the growth of three different M. tuberculosis pathogens and in a comparison with the isoniazid drug as a standard anti-tuberculosis drug.					
36657855	3	29	theme	small	585:589	arg1	size					600:603	a small particle size	583:603	a small particle size of ~22 nm	583:613	TEM data has proved the formation of uniformly distributed TCNPs on graphene surfaces with a small particle size of ~22 nm compared with that of pure nanoparticles (~30 nm).					
36657855	2	30	theme	various	418:424	arg1	techniques					437:446	various analytical techniques	418:446	various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM	418:489	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	2	30	theme	various	418:424	arg1	UV/vis					470:475	UV/vis	470:475	UV/vis	470:475	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	2	30	theme	various	418:424	arg1	SEM					478:480	SEM	478:480	SEM	478:480	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	2	30	theme	various	418:424	arg1	1H-NMR					456:461	1H-NMR	456:461	1H-NMR	456:461	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	2	30	theme	various	418:424	arg1	FTIR					464:467	FTIR	464:467	FTIR	464:467	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	2	30	theme	various	418:424	arg1	TEM					487:489	TEM	487:489	TEM	487:489	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	0	31	theme	TMC-CMS	21:27	arg1	nanoparticles					29:41	TMC-CMS nanoparticles	21:41	TMC-CMS nanoparticles	21:41	Green engineering of TMC-CMS nanoparticles decorated graphene sheets for targeting M. tuberculosis.					
36657855	7	32	theme	@	1275:1275	arg1	GRP					1276:1278	the TCNPs@GRP	1266:1278	the TCNPs@GRP	1266:1278	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	5	33	theme	XDR	1000:1002	arg1	tuberculosis					1007:1018	XDR M. tuberculosis	1000:1018	XDR M. tuberculosis	1000:1018	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	1	34	theme	current	104:110	arg1	work					112:115	Our current work	100:115	Our current work	100:115	Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach.					
36657855	5	35	theme	MDR	991:993	arg1	pathogens					1020:1028	sensitive, MDR, and XDR M. tuberculosis pathogens	980:1028	sensitive, MDR, and XDR M. tuberculosis pathogens	980:1028	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	5	36	theme	3.9	931:933	arg1	values					915:920	MIC values	911:920	MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens	911:1028	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	4	37	theme	pathogens	786:794	arg1	growth					744:749	the growth	740:749	the growth of three different M. tuberculosis pathogens	740:794	The inhibitory activity of these developed materials was examined against the growth of three different M. tuberculosis pathogens and in a comparison with the isoniazid drug as a standard anti-tuberculosis drug.					
36657855	4	38	theme	materials	709:717	arg1	activity					681:688	The inhibitory activity	666:688	The inhibitory activity of these developed materials	666:717	The inhibitory activity of these developed materials was examined against the growth of three different M. tuberculosis pathogens and in a comparison with the isoniazid drug as a standard anti-tuberculosis drug.					
36657855	4	39	dep	M.	770:771	arg1	tuberculosis					773:784	tuberculosis	773:784	tuberculosis	773:784	The inhibitory activity of these developed materials was examined against the growth of three different M. tuberculosis pathogens and in a comparison with the isoniazid drug as a standard anti-tuberculosis drug.					
36657855	5	40	theme	TCNPs	882:886	arg1	composite					892:900	The TCNPs@GRP composite	878:900	The TCNPs@GRP composite	878:900	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	1	41	theme	biocompatible	207:219	arg1	sheet					230:234	a highly biocompatible graphene sheet	198:234	a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach	198:342	Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach.					
36657855	0	42	theme	nanoparticles	29:41	arg1	engineering					6:16	Green engineering	0:16	Green engineering of TMC-CMS nanoparticles	0:41	Green engineering of TMC-CMS nanoparticles decorated graphene sheets for targeting M. tuberculosis.					
36657855	1	43	theme	ultrasonication	319:333	arg1	approach					335:342	one-pot, green, cost-effective ultrasonication approach	288:342	one-pot, green, cost-effective ultrasonication approach	288:342	Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach.					
36657855	7	44	contain	have	1348:1351	arg1	Cytotoxicity					1250:1261	Cytotoxicity	1250:1261	Cytotoxicity of the TCNPs@GRP	1250:1278	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	7	44	contain	have	1348:1351	arg2	viability					1358:1366	cell viability	1353:1366	cell viability of 100%	1353:1374	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	3	45	theme	pure	637:640	arg1	~30 nm					657:662	~30 nm	657:662	~30 nm	657:662	TEM data has proved the formation of uniformly distributed TCNPs on graphene surfaces with a small particle size of ~22 nm compared with that of pure nanoparticles (~30 nm).					
36657855	3	45	theme	pure	637:640	arg1	nanoparticles					642:654	pure nanoparticles	637:654	pure nanoparticles (~30 nm)	637:663	TEM data has proved the formation of uniformly distributed TCNPs on graphene surfaces with a small particle size of ~22 nm compared with that of pure nanoparticles (~30 nm).					
36657855	3	46	theme	~22 nm	608:613	arg1	size					600:603	a small particle size	583:603	a small particle size of ~22 nm	583:613	TEM data has proved the formation of uniformly distributed TCNPs on graphene surfaces with a small particle size of ~22 nm compared with that of pure nanoparticles (~30 nm).					
36657855	7	47	theme	normal	1301:1306	arg1	WI38					1325:1328	WI38	1325:1328	WI38	1325:1328	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	7	47	theme	normal	1301:1306	arg1	lines					1318:1322	normal lung cell lines	1301:1322	normal lung cell lines (WI38)	1301:1329	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	1	48	theme	graphene	221:228	arg1	sheet					230:234	a highly biocompatible graphene sheet	198:234	a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach	198:342	Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach.					
36657855	5	49	theme	isoniazid	1091:1099	arg1	drug					1101:1104	the isoniazid drug	1087:1104	the isoniazid drug (0.24, 0, 0 μg/mL)	1087:1123	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	5	50	theme	tuberculosis	1007:1018	arg1	pathogens					1020:1028	sensitive, MDR, and XDR M. tuberculosis pathogens	980:1028	sensitive, MDR, and XDR M. tuberculosis pathogens	980:1028	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	3	51	theme	particle	591:598	arg1	size					600:603	a small particle size	583:603	a small particle size of ~22 nm	583:613	TEM data has proved the formation of uniformly distributed TCNPs on graphene surfaces with a small particle size of ~22 nm compared with that of pure nanoparticles (~30 nm).					
36657855	2	52	theme	materials	377:385	arg1	microstructure					349:362	The microstructure	345:362	The microstructure of as-formed materials	345:385	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	0	53	theme	graphene	53:60	arg1	sheets					62:67	graphene sheets	53:67	graphene sheets for targeting M. tuberculosis	53:97	Green engineering of TMC-CMS nanoparticles decorated graphene sheets for targeting M. tuberculosis.					
36657855	5	54	theme	M.	1004:1005	arg1	tuberculosis					1007:1018	XDR M. tuberculosis	1000:1018	XDR M. tuberculosis	1000:1018	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	4	55	theme	isoniazid	825:833	arg1	drug					835:838	the isoniazid drug	821:838	the isoniazid drug as a standard anti-tuberculosis drug	821:875	The inhibitory activity of these developed materials was examined against the growth of three different M. tuberculosis pathogens and in a comparison with the isoniazid drug as a standard anti-tuberculosis drug.					
36657855	2	56	theme	as-formed	367:375	arg1	materials					377:385	as-formed materials	367:385	as-formed materials	367:385	The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as 1H-NMR, FTIR, UV/vis, SEM, and TEM.					
36657855	7	57	theme	GRP	1276:1278	arg1	Cytotoxicity					1250:1261	Cytotoxicity	1250:1261	Cytotoxicity of the TCNPs@GRP	1250:1278	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	6	58	theme	graphene	1229:1236	arg1	nanosheets					1238:1247	graphene nanosheets	1229:1247	graphene nanosheets	1229:1247	This reveals a considerable synergism in the antituberculosis activity between TCNPs and graphene nanosheets.					
36657855	1	59	dep	one-pot	288:294	arg1	cost-effective					304:317	cost-effective	304:317	cost-effective	304:317	Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach.					
36657855	1	59	dep	one-pot	288:294	arg1	green					297:301	green	297:301	green	297:301	Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach.					
36657855	7	60	theme	cell	1313:1316	arg1	WI38					1325:1328	WI38	1325:1328	WI38	1325:1328	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	7	60	theme	cell	1313:1316	arg1	lines					1318:1322	normal lung cell lines	1301:1322	normal lung cell lines (WI38)	1301:1329	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	3	61	theme	TEM	492:494	arg1	data					496:499	TEM data	492:499	TEM data	492:499	TEM data has proved the formation of uniformly distributed TCNPs on graphene surfaces with a small particle size of ~22 nm compared with that of pure nanoparticles (~30 nm).					
36657855	5	62	theme	pathogens	1020:1028	arg1	growth					970:975	the growth	966:975	the growth of sensitive, MDR, and XDR M. tuberculosis pathogens	966:1028	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	5	63	theme	7.81 μg/mL	940:949	arg1	values					915:920	MIC values	911:920	MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens	911:1028	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	5	64	theme	GRP	888:890	arg1	composite					892:900	The TCNPs@GRP composite	878:900	The TCNPs@GRP composite	878:900	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	5	65	theme	0.98	925:928	arg1	values					915:920	MIC values	911:920	MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens	911:1028	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	0	66	theme	targeting	73:81	arg1	tuberculosis					86:97	targeting M. tuberculosis	73:97	targeting M. tuberculosis	73:97	Green engineering of TMC-CMS nanoparticles decorated graphene sheets for targeting M. tuberculosis.					
36657855	5	67	theme	sensitive	980:988	arg1	pathogens					1020:1028	sensitive, MDR, and XDR M. tuberculosis pathogens	980:1028	sensitive, MDR, and XDR M. tuberculosis pathogens	980:1028	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	5	68	theme	@	887:887	arg1	composite					892:900	The TCNPs@GRP composite	878:900	The TCNPs@GRP composite	878:900	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	7	69	theme	TCNPs	1270:1274	arg1	GRP					1276:1278	the TCNPs@GRP	1266:1278	the TCNPs@GRP	1266:1278	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	5	70	dep	TCNPs	1051:1055	arg1	>125 μg/mL					1071:1080	>125 μg/mL	1071:1080	>125 μg/mL	1071:1080	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	5	70	dep	TCNPs	1051:1055	arg1	7.81					1058:1061	7.81	1058:1061	7.81	1058:1061	The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively.					
36657855	7	71	theme	0.98-7.81 μg/mL	1408:1422	arg1	range					1399:1403	the concentration range	1381:1403	the concentration range of 0.98-7.81 μg/mL	1381:1422	Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.					
36657855	1	72	theme	new	149:151	arg1	type					153:156	a new type	147:156	a new type of antibacterial composite	147:183	Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach.					
36657855	1	73	theme	TMC-CMS	251:257	arg1	nanoparticles					264:276	TMC-CMS IPNs nanoparticles	251:276	TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach	251:342	Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach.					
35245572	0	0	theme	ion-imprinted	56:68	arg1	chitosan/kaolin					85:99	ion-imprinted honeycomb-like chitosan/kaolin	56:99	ion-imprinted honeycomb-like chitosan/kaolin	56:99	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin clay composite foams.					
35245572	5	1	from	pH 5.0	986:991	arg1	U					967:967	U	967:967	U(VI) at 298 K and pH 5.0	967:991	The sorption isotherms was well-fitted with Langmuir model, and the maximum sorption capacity of ICK-2 was evaluated as 286.85 mg/g for U(VI) at 298 K and pH 5.0.					
35245572	7	2	theme	FTIR	1064:1067	arg1	results					1077:1083	The FTIR and XPS results	1060:1083	The FTIR and XPS results	1060:1083	The FTIR and XPS results suggest that both amine and hydroxyl groups are responsible for U(VI) coordination.					
35245572	5	3	theme	Langmuir	875:882	arg1	model					884:888	Langmuir model	875:888	Langmuir model	875:888	The sorption isotherms was well-fitted with Langmuir model, and the maximum sorption capacity of ICK-2 was evaluated as 286.85 mg/g for U(VI) at 298 K and pH 5.0.					
35245572	8	4	dep	separation	1300:1309	arg1	VI					1296:1297	VI	1296:1297	VI	1296:1297	The ICK-2 presents high sorption capacity, good selectivity and fast kinetic rate, and thus it has potential application for U(VI) separation from radioactive wastewater.					
35245572	1	5	theme	ecosystem	243:251	arg1	stability					253:261	ecosystem stability	243:261	ecosystem stability	243:261	The radioactive pollution caused by the discharge of radioactive wastewater poses a serious threat to public health and ecosystem stability owing to its long-term detriments.					
35245572	8	6	theme	fast	1233:1236	arg1	rate					1246:1249	fast kinetic rate	1233:1249	fast kinetic rate	1233:1249	The ICK-2 presents high sorption capacity, good selectivity and fast kinetic rate, and thus it has potential application for U(VI) separation from radioactive wastewater.					
35245572	0	7	from	biosorption	10:20	arg1	solution					44:51	aqueous solution	36:51	aqueous solution	36:51	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin clay composite foams.					
35245572	2	8	theme	ICK	361:363	arg1	foams					376:380	the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams	306:380	the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams	306:380	Herein, the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams were successfully fabricated and applied to the selective biosorption of U(VI) from aqueous solution.					
35245572	5	9	theme	sorption	907:914	arg1	capacity					916:923	the maximum sorption capacity	895:923	the maximum sorption capacity of ICK-2	895:932	The sorption isotherms was well-fitted with Langmuir model, and the maximum sorption capacity of ICK-2 was evaluated as 286.85 mg/g for U(VI) at 298 K and pH 5.0.					
35245572	5	9	theme	sorption	907:914	arg1	286.85 mg/g					951:961	286.85 mg/g	951:961	286.85 mg/g for U(VI) at 298 K and pH 5.0	951:991	The sorption isotherms was well-fitted with Langmuir model, and the maximum sorption capacity of ICK-2 was evaluated as 286.85 mg/g for U(VI) at 298 K and pH 5.0.					
35245572	8	10	theme	radioactive	1316:1326	arg1	wastewater					1328:1337	radioactive wastewater	1316:1337	radioactive wastewater	1316:1337	The ICK-2 presents high sorption capacity, good selectivity and fast kinetic rate, and thus it has potential application for U(VI) separation from radioactive wastewater.					
35245572	7	11	theme	U	1149:1149	arg1	coordination					1155:1166	U(VI) coordination	1149:1166	U(VI) coordination	1149:1166	The FTIR and XPS results suggest that both amine and hydroxyl groups are responsible for U(VI) coordination.					
35245572	5	12	theme	ICK-2	928:932	arg1	capacity					916:923	the maximum sorption capacity	895:923	the maximum sorption capacity of ICK-2	895:932	The sorption isotherms was well-fitted with Langmuir model, and the maximum sorption capacity of ICK-2 was evaluated as 286.85 mg/g for U(VI) at 298 K and pH 5.0.					
35245572	5	12	theme	ICK-2	928:932	arg1	286.85 mg/g					951:961	286.85 mg/g	951:961	286.85 mg/g for U(VI) at 298 K and pH 5.0	951:991	The sorption isotherms was well-fitted with Langmuir model, and the maximum sorption capacity of ICK-2 was evaluated as 286.85 mg/g for U(VI) at 298 K and pH 5.0.					
35245572	0	13	theme	honeycomb-like	70:83	arg1	chitosan/kaolin					85:99	ion-imprinted honeycomb-like chitosan/kaolin	56:99	ion-imprinted honeycomb-like chitosan/kaolin	56:99	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin clay composite foams.					
35245572	6	14	theme	pseudo-second	1033:1045	arg1	model					1053:1057	pseudo-second order model	1033:1057	pseudo-second order model	1033:1057	The kinetic data could be described by pseudo-second order model.					
35245572	2	15	theme	honeycomb-like	324:337	arg1	foams					376:380	the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams	306:380	the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams	306:380	Herein, the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams were successfully fabricated and applied to the selective biosorption of U(VI) from aqueous solution.					
35245572	0	16	theme	composite	106:114	arg1	foams					116:120	composite foams	106:120	composite foams	106:120	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin clay composite foams.					
35245572	4	17	theme	target	818:823	arg1	ions					825:828	the target ions	814:828	the target ions	814:828	As compared to the non-imprinted sorbent (NICK-2), the ion-imprinted sorbent (ICK-2) presents higher sorption and better selectivity since it can smartly recognize the target ions.					
35245572	8	18	theme	high	1188:1191	arg1	capacity					1202:1209	high sorption capacity	1188:1209	high sorption capacity	1188:1209	The ICK-2 presents high sorption capacity, good selectivity and fast kinetic rate, and thus it has potential application for U(VI) separation from radioactive wastewater.					
35245572	0	19	theme	Selective	0:8	arg1	biosorption					10:20	Selective biosorption	0:20	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin	0:99	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin clay composite foams.					
35245572	5	20	theme	sorption	835:842	arg1	isotherms					844:852	The sorption isotherms	831:852	The sorption isotherms	831:852	The sorption isotherms was well-fitted with Langmuir model, and the maximum sorption capacity of ICK-2 was evaluated as 286.85 mg/g for U(VI) at 298 K and pH 5.0.					
35245572	2	21	theme	ion-imprinted	310:322	arg1	foams					376:380	the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams	306:380	the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams	306:380	Herein, the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams were successfully fabricated and applied to the selective biosorption of U(VI) from aqueous solution.					
35245572	1	22	theme	radioactive	176:186	arg1	wastewater					188:197	radioactive wastewater	176:197	radioactive wastewater	176:197	The radioactive pollution caused by the discharge of radioactive wastewater poses a serious threat to public health and ecosystem stability owing to its long-term detriments.					
35245572	2	23	theme	U	455:455	arg1	biosorption					440:450	the selective biosorption	426:450	the selective biosorption of U	426:455	Herein, the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams were successfully fabricated and applied to the selective biosorption of U(VI) from aqueous solution.					
35245572	4	24	theme	ion-imprinted	705:717	arg1	ICK-2					728:732	ICK-2	728:732	ICK-2	728:732	As compared to the non-imprinted sorbent (NICK-2), the ion-imprinted sorbent (ICK-2) presents higher sorption and better selectivity since it can smartly recognize the target ions.					
35245572	4	24	theme	ion-imprinted	705:717	arg1	sorbent					719:725	the ion-imprinted sorbent	701:725	the ion-imprinted sorbent (ICK-2)	701:733	As compared to the non-imprinted sorbent (NICK-2), the ion-imprinted sorbent (ICK-2) presents higher sorption and better selectivity since it can smartly recognize the target ions.					
35245572	5	25	from	298 K	976:980	arg1	U					967:967	U	967:967	U(VI) at 298 K and pH 5.0	967:991	The sorption isotherms was well-fitted with Langmuir model, and the maximum sorption capacity of ICK-2 was evaluated as 286.85 mg/g for U(VI) at 298 K and pH 5.0.					
35245572	1	26	theme	wastewater	188:197	arg1	discharge					163:171	the discharge	159:171	the discharge of radioactive wastewater	159:197	The radioactive pollution caused by the discharge of radioactive wastewater poses a serious threat to public health and ecosystem stability owing to its long-term detriments.					
35245572	0	27	theme	U	25:25	arg1	biosorption					10:20	Selective biosorption	0:20	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin	0:99	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin clay composite foams.					
35245572	1	28	theme	long-term	276:284	arg1	detriments					286:295	its long-term detriments	272:295	its long-term detriments	272:295	The radioactive pollution caused by the discharge of radioactive wastewater poses a serious threat to public health and ecosystem stability owing to its long-term detriments.					
35245572	5	29	theme	maximum	899:905	arg1	capacity					916:923	the maximum sorption capacity	895:923	the maximum sorption capacity of ICK-2	895:932	The sorption isotherms was well-fitted with Langmuir model, and the maximum sorption capacity of ICK-2 was evaluated as 286.85 mg/g for U(VI) at 298 K and pH 5.0.					
35245572	5	29	theme	maximum	899:905	arg1	286.85 mg/g					951:961	286.85 mg/g	951:961	286.85 mg/g for U(VI) at 298 K and pH 5.0	951:991	The sorption isotherms was well-fitted with Langmuir model, and the maximum sorption capacity of ICK-2 was evaluated as 286.85 mg/g for U(VI) at 298 K and pH 5.0.					
35245572	3	30	theme	various	531:537	arg1	foams					543:547	various ICK foams	531:547	various ICK foams owing to its well-developed honeycomb-like structure and the presence of abundant functional groups	531:647	It was found that the ICK-2 was the best among various ICK foams owing to its well-developed honeycomb-like structure and the presence of abundant functional groups.					
35245572	8	31	from	wastewater	1328:1337	arg1	separation					1300:1309	U(VI) separation	1294:1309	U(VI) separation from radioactive wastewater	1294:1337	The ICK-2 presents high sorption capacity, good selectivity and fast kinetic rate, and thus it has potential application for U(VI) separation from radioactive wastewater.					
35245572	2	32	theme	selective	430:438	arg1	biosorption					440:450	the selective biosorption	426:450	the selective biosorption of U	426:455	Herein, the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams were successfully fabricated and applied to the selective biosorption of U(VI) from aqueous solution.					
35245572	7	33	theme	amine	1103:1107	arg1	groups					1122:1127	both amine and hydroxyl groups	1098:1127	groups	1122:1127	The FTIR and XPS results suggest that both amine and hydroxyl groups are responsible for U(VI) coordination.					
35245572	3	34	theme	functional	631:640	arg1	groups					642:647	abundant functional groups	622:647	abundant functional groups	622:647	It was found that the ICK-2 was the best among various ICK foams owing to its well-developed honeycomb-like structure and the presence of abundant functional groups.					
35245572	3	35	theme	ICK	539:541	arg1	foams					543:547	various ICK foams	531:547	various ICK foams owing to its well-developed honeycomb-like structure and the presence of abundant functional groups	531:647	It was found that the ICK-2 was the best among various ICK foams owing to its well-developed honeycomb-like structure and the presence of abundant functional groups.					
35245572	8	36	theme	kinetic	1238:1244	arg1	rate					1246:1249	fast kinetic rate	1233:1249	fast kinetic rate	1233:1249	The ICK-2 presents high sorption capacity, good selectivity and fast kinetic rate, and thus it has potential application for U(VI) separation from radioactive wastewater.					
35245572	2	37	theme	aqueous	466:472	arg1	solution					474:481	aqueous solution	466:481	aqueous solution	466:481	Herein, the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams were successfully fabricated and applied to the selective biosorption of U(VI) from aqueous solution.					
35245572	3	38	theme	groups	642:647	arg1	structure					592:600	its well-developed honeycomb-like structure	558:600	its well-developed honeycomb-like structure	558:600	It was found that the ICK-2 was the best among various ICK foams owing to its well-developed honeycomb-like structure and the presence of abundant functional groups.					
35245572	3	38	theme	groups	642:647	arg1	presence					610:617	the presence	606:617	the presence of abundant functional groups	606:647	It was found that the ICK-2 was the best among various ICK foams owing to its well-developed honeycomb-like structure and the presence of abundant functional groups.					
35245572	3	39	theme	well-developed	562:575	arg1	structure					592:600	its well-developed honeycomb-like structure	558:600	its well-developed honeycomb-like structure	558:600	It was found that the ICK-2 was the best among various ICK foams owing to its well-developed honeycomb-like structure and the presence of abundant functional groups.					
35245572	1	40	theme	serious	207:213	arg1	threat					215:220	a serious threat	205:220	a serious threat to public health and ecosystem stability owing to its long-term detriments	205:295	The radioactive pollution caused by the discharge of radioactive wastewater poses a serious threat to public health and ecosystem stability owing to its long-term detriments.					
35245572	0	41	from	solution	44:51	arg1	U					25:25	U	25:25	U(VI) from aqueous solution	25:51	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin clay composite foams.					
35245572	0	41	from	solution	44:51	arg1	biosorption					10:20	Selective biosorption	0:20	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin	0:99	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin clay composite foams.					
35245572	3	42	theme	honeycomb-like	577:590	arg1	structure					592:600	its well-developed honeycomb-like structure	558:600	its well-developed honeycomb-like structure	558:600	It was found that the ICK-2 was the best among various ICK foams owing to its well-developed honeycomb-like structure and the presence of abundant functional groups.					
35245572	5	43	dep	U	967:967	arg1	VI					969:970	VI	969:970	VI	969:970	The sorption isotherms was well-fitted with Langmuir model, and the maximum sorption capacity of ICK-2 was evaluated as 286.85 mg/g for U(VI) at 298 K and pH 5.0.					
35245572	7	44	theme	XPS	1073:1075	arg1	results					1077:1083	The FTIR and XPS results	1060:1083	The FTIR and XPS results	1060:1083	The FTIR and XPS results suggest that both amine and hydroxyl groups are responsible for U(VI) coordination.					
35245572	3	45	theme	abundant	622:629	arg1	groups					642:647	abundant functional groups	622:647	abundant functional groups	622:647	It was found that the ICK-2 was the best among various ICK foams owing to its well-developed honeycomb-like structure and the presence of abundant functional groups.					
35245572	8	46	theme	potential	1268:1276	arg1	application					1278:1288	potential application	1268:1288	potential application for U(VI) separation from radioactive wastewater	1268:1337	The ICK-2 presents high sorption capacity, good selectivity and fast kinetic rate, and thus it has potential application for U(VI) separation from radioactive wastewater.					
35245572	8	47	theme	good	1212:1215	arg1	selectivity					1217:1227	good selectivity	1212:1227	good selectivity	1212:1227	The ICK-2 presents high sorption capacity, good selectivity and fast kinetic rate, and thus it has potential application for U(VI) separation from radioactive wastewater.					
35245572	2	48	dep	solution	474:481	arg1	VI					457:458	VI	457:458	VI	457:458	Herein, the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams were successfully fabricated and applied to the selective biosorption of U(VI) from aqueous solution.					
35245572	2	49	theme	clay	355:358	arg1	foams					376:380	the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams	306:380	the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams	306:380	Herein, the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams were successfully fabricated and applied to the selective biosorption of U(VI) from aqueous solution.					
35245572	4	50	theme	better	764:769	arg1	selectivity					771:781	better selectivity	764:781	better selectivity	764:781	As compared to the non-imprinted sorbent (NICK-2), the ion-imprinted sorbent (ICK-2) presents higher sorption and better selectivity since it can smartly recognize the target ions.					
35245572	0	51	dep	U	25:25	arg1	VI					27:28	VI	27:28	VI	27:28	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin clay composite foams.					
35245572	2	52	theme	chitosan/kaolin	339:353	arg1	foams					376:380	the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams	306:380	the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams	306:380	Herein, the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams were successfully fabricated and applied to the selective biosorption of U(VI) from aqueous solution.					
35245572	8	53	theme	U	1294:1294	arg1	separation					1300:1309	U(VI) separation	1294:1309	U(VI) separation from radioactive wastewater	1294:1337	The ICK-2 presents high sorption capacity, good selectivity and fast kinetic rate, and thus it has potential application for U(VI) separation from radioactive wastewater.					
35245572	8	54	theme	sorption	1193:1200	arg1	capacity					1202:1209	high sorption capacity	1188:1209	high sorption capacity	1188:1209	The ICK-2 presents high sorption capacity, good selectivity and fast kinetic rate, and thus it has potential application for U(VI) separation from radioactive wastewater.					
35245572	4	55	theme	higher	744:749	arg1	sorption					751:758	higher sorption	744:758	higher sorption	744:758	As compared to the non-imprinted sorbent (NICK-2), the ion-imprinted sorbent (ICK-2) presents higher sorption and better selectivity since it can smartly recognize the target ions.					
35245572	1	56	theme	radioactive	127:137	arg1	pollution					139:147	The radioactive pollution	123:147	The radioactive pollution caused by the discharge of radioactive wastewater	123:197	The radioactive pollution caused by the discharge of radioactive wastewater poses a serious threat to public health and ecosystem stability owing to its long-term detriments.					
35245572	1	57	theme	public	225:230	arg1	health					232:237	public health	225:237	public health	225:237	The radioactive pollution caused by the discharge of radioactive wastewater poses a serious threat to public health and ecosystem stability owing to its long-term detriments.					
35245572	6	58	theme	order	1047:1051	arg1	model					1053:1057	pseudo-second order model	1033:1057	pseudo-second order model	1033:1057	The kinetic data could be described by pseudo-second order model.					
35245572	6	59	theme	kinetic	998:1004	arg1	data					1006:1009	The kinetic data	994:1009	The kinetic data	994:1009	The kinetic data could be described by pseudo-second order model.					
35245572	4	60	theme	non-imprinted	669:681	arg1	sorbent					683:689	the non-imprinted sorbent	665:689	the non-imprinted sorbent (NICK-2)	665:698	As compared to the non-imprinted sorbent (NICK-2), the ion-imprinted sorbent (ICK-2) presents higher sorption and better selectivity since it can smartly recognize the target ions.					
35245572	4	60	theme	non-imprinted	669:681	arg1	NICK-2					692:697	NICK-2	692:697	NICK-2	692:697	As compared to the non-imprinted sorbent (NICK-2), the ion-imprinted sorbent (ICK-2) presents higher sorption and better selectivity since it can smartly recognize the target ions.					
35245572	7	61	dep	coordination	1155:1166	arg1	VI					1151:1152	VI	1151:1152	VI	1151:1152	The FTIR and XPS results suggest that both amine and hydroxyl groups are responsible for U(VI) coordination.					
35245572	0	62	theme	aqueous	36:42	arg1	solution					44:51	aqueous solution	36:51	aqueous solution	36:51	Selective biosorption of U(VI) from aqueous solution by ion-imprinted honeycomb-like chitosan/kaolin clay composite foams.					
35245572	2	63	theme	composite	366:374	arg1	foams					376:380	the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams	306:380	the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams	306:380	Herein, the ion-imprinted honeycomb-like chitosan/kaolin clay (ICK) composite foams were successfully fabricated and applied to the selective biosorption of U(VI) from aqueous solution.					
35245572	7	64	theme	hydroxyl	1113:1120	arg1	groups					1122:1127	both amine and hydroxyl groups	1098:1127	groups	1122:1127	The FTIR and XPS results suggest that both amine and hydroxyl groups are responsible for U(VI) coordination.					
35245572	8	65	contain	has	1264:1266	arg1	it					1261:1262	it	1261:1262	it	1261:1262	The ICK-2 presents high sorption capacity, good selectivity and fast kinetic rate, and thus it has potential application for U(VI) separation from radioactive wastewater.					
35245572	8	65	contain	has	1264:1266	arg2	application					1278:1288	potential application	1268:1288	potential application for U(VI) separation from radioactive wastewater	1268:1337	The ICK-2 presents high sorption capacity, good selectivity and fast kinetic rate, and thus it has potential application for U(VI) separation from radioactive wastewater.					
36674765	10	0	theme	mechanical	1455:1464	arg1	properties					1466:1475	mechanical properties	1455:1475	mechanical properties	1455:1475	Thus, serosal adhesion combined with excellent stability and mechanical properties in physiological environments appeared to be advantages of the FeP hydrogel, demonstrating it to be a promising bioadhesive for tissue engineering.					
36674765	8	1	theme	serosal	1203:1209	arg1	adhesion					1211:1218	the serosal adhesion	1199:1218	the serosal adhesion of the FeP hydrogel	1199:1238	Serum proteins and peritoneal leukocytes did not interfere with the serosal adhesion of the FeP hydrogel.					
36674765	4	2	theme	surface	531:537	arg1	microrelief					539:549	surface microrelief	531:549	surface microrelief	531:549	The mechanical properties and surface microrelief of the pectin hydrogel were influenced by the type and concentration of the cross-linking cations.					
36674765	10	3	theme	serosal	1400:1406	arg1	adhesion					1408:1415	serosal adhesion	1400:1415	serosal adhesion combined with excellent stability and mechanical properties in physiological environments	1400:1505	Thus, serosal adhesion combined with excellent stability and mechanical properties in physiological environments appeared to be advantages of the FeP hydrogel, demonstrating it to be a promising bioadhesive for tissue engineering.					
36674765	6	4	theme	surface	783:789	arg1	pattern					791:797	the characteristic surface pattern	764:797	the characteristic surface pattern of FeP hydrogel and its denser internal structure	764:847	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	10	5	from	properties	1466:1475	arg1	environments					1494:1505	physiological environments	1480:1505	physiological environments	1480:1505	Thus, serosal adhesion combined with excellent stability and mechanical properties in physiological environments appeared to be advantages of the FeP hydrogel, demonstrating it to be a promising bioadhesive for tissue engineering.					
36674765	6	6	theme	cross-linked	866:877	arg1	hydrogel					879:886	Ca2+ cross-linked hydrogel	861:886	Ca2+ cross-linked hydrogel	861:886	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	7	7	theme	adhesion	931:938	arg1	medium					940:945	the adhesion medium	927:945	the adhesion medium	927:945	The effect of the salt composition of the adhesion medium was shown since the FeP hydrogel's adhesion to the serosa was lower in physiological solutions than in water, and adhesion in Hanks' solution was higher than in phosphate buffered saline.					
36674765	6	8	theme	characteristic	768:781	arg1	pattern					791:797	the characteristic surface pattern	764:797	the characteristic surface pattern of FeP hydrogel and its denser internal structure	764:847	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	2	9	theme	hydrogel	261:268	arg1	adhesivity					239:248	The adhesivity	235:248	The adhesivity of the FeP hydrogel, which was cross-linked by Fe3+ cations,	235:309	The adhesivity of the FeP hydrogel, which was cross-linked by Fe3+ cations, exceeded that of hydrogels cross-linked by Ca2+, Zn2+, and Al3+ cations.					
36674765	10	10	from	stability	1441:1449	arg1	environments					1494:1505	physiological environments	1480:1505	physiological environments	1480:1505	Thus, serosal adhesion combined with excellent stability and mechanical properties in physiological environments appeared to be advantages of the FeP hydrogel, demonstrating it to be a promising bioadhesive for tissue engineering.					
36674765	0	11	theme	Fe3+	83:86	arg1	Ions					88:91	Fe3+ Ions	83:91	Fe3+ Ions	83:91	Serosal Adhesion Ex Vivo of Hydrogels Prepared from Apple Pectin Cross-Linked with Fe3+ Ions.					
36674765	6	12	theme	Ca2+	861:864	arg1	hydrogel					879:886	Ca2+ cross-linked hydrogel	861:886	Ca2+ cross-linked hydrogel	861:886	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	7	13	theme	composition	912:922	arg1	effect					893:898	The effect	889:898	The effect of the salt composition of the adhesion medium	889:945	The effect of the salt composition of the adhesion medium was shown since the FeP hydrogel's adhesion to the serosa was lower in physiological solutions than in water, and adhesion in Hanks' solution was higher than in phosphate buffered saline.					
36674765	2	14	theme	FeP	257:259	arg1	hydrogel					261:268	the FeP hydrogel	253:268	the FeP hydrogel	253:268	The adhesivity of the FeP hydrogel, which was cross-linked by Fe3+ cations, exceeded that of hydrogels cross-linked by Ca2+, Zn2+, and Al3+ cations.					
36674765	8	15	theme	hydrogel	1231:1238	arg1	adhesion					1211:1218	the serosal adhesion	1199:1218	the serosal adhesion of the FeP hydrogel	1199:1238	Serum proteins and peritoneal leukocytes did not interfere with the serosal adhesion of the FeP hydrogel.					
36674765	5	16	theme	elastic	686:692	arg1	gel					694:696	a harder and more elastic gel	668:696	a harder and more elastic gel than Ca2+ cations	668:714	Fe3+ cations form a harder and more elastic gel than Ca2+ cations.					
36674765	1	17	theme	esterified	183:192	arg1	pectin					194:199	cross-linked low-methyl esterified pectin	159:199	cross-linked low-methyl esterified pectin	159:199	The study aims to investigate the adhesion of a hydrogel made of cross-linked low-methyl esterified pectin to rat intestinal serosa ex vivo.					
36674765	3	18	theme	hydrogel	477:484	arg1	adhesion					454:461	the adhesion	450:461	the adhesion of the pectin hydrogel to the serosa	450:498	The concentration of the cross-linking cation failed to influence the adhesion of the pectin hydrogel to the serosa.					
36674765	1	19	theme	hydrogel	142:149	arg1	adhesion					128:135	the adhesion	124:135	the adhesion of a hydrogel made of cross-linked low-methyl esterified pectin to rat intestinal serosa	124:224	The study aims to investigate the adhesion of a hydrogel made of cross-linked low-methyl esterified pectin to rat intestinal serosa ex vivo.					
36674765	10	20	theme	FeP	1540:1542	arg1	hydrogel					1544:1551	the FeP hydrogel	1536:1551	the FeP hydrogel	1536:1551	Thus, serosal adhesion combined with excellent stability and mechanical properties in physiological environments appeared to be advantages of the FeP hydrogel, demonstrating it to be a promising bioadhesive for tissue engineering.					
36674765	1	21	link	cross-linked	159:170	arg1	pectin					194:199	cross-linked low-methyl esterified pectin	159:199	cross-linked low-methyl esterified pectin	159:199	The study aims to investigate the adhesion of a hydrogel made of cross-linked low-methyl esterified pectin to rat intestinal serosa ex vivo.					
36674765	7	22	theme	phosphate	1108:1116	arg1	saline					1127:1132	phosphate buffered saline	1108:1132	phosphate buffered saline	1108:1132	The effect of the salt composition of the adhesion medium was shown since the FeP hydrogel's adhesion to the serosa was lower in physiological solutions than in water, and adhesion in Hanks' solution was higher than in phosphate buffered saline.					
36674765	2	23	theme	Ca2+	354:357	arg1	cations					375:381	Ca2+, Zn2+, and Al3+ cations	354:381	Ca2+, Zn2+, and Al3+ cations	354:381	The adhesivity of the FeP hydrogel, which was cross-linked by Fe3+ cations, exceeded that of hydrogels cross-linked by Ca2+, Zn2+, and Al3+ cations.					
36674765	6	24	theme	structure	839:847	arg1	pattern					791:797	the characteristic surface pattern	764:797	the characteristic surface pattern of FeP hydrogel and its denser internal structure	764:847	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	6	25	theme	microscopy	735:744	arg1	analysis					746:753	Scanning electron microscopy analysis	717:753	Scanning electron microscopy analysis	717:753	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	0	26	theme	Serosal	0:6	arg1	Adhesion					8:15	Serosal Adhesion	0:15	Serosal Adhesion Ex Vivo of Hydrogels Prepared from Apple Pectin	0:63	Serosal Adhesion Ex Vivo of Hydrogels Prepared from Apple Pectin Cross-Linked with Fe3+ Ions.					
36674765	6	27	theme	electron	726:733	arg1	microscopy					735:744	Scanning electron microscopy	717:744	Scanning electron microscopy analysis	717:753	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	1	28	theme	cross-linked	159:170	arg1	pectin					194:199	cross-linked low-methyl esterified pectin	159:199	cross-linked low-methyl esterified pectin	159:199	The study aims to investigate the adhesion of a hydrogel made of cross-linked low-methyl esterified pectin to rat intestinal serosa ex vivo.					
36674765	8	29	theme	Serum	1135:1139	arg1	proteins					1141:1148	Serum proteins	1135:1148	Serum proteins	1135:1148	Serum proteins and peritoneal leukocytes did not interfere with the serosal adhesion of the FeP hydrogel.					
36674765	10	30	theme	tissue	1605:1610	arg1	engineering					1612:1622	tissue engineering	1605:1622	tissue engineering	1605:1622	Thus, serosal adhesion combined with excellent stability and mechanical properties in physiological environments appeared to be advantages of the FeP hydrogel, demonstrating it to be a promising bioadhesive for tissue engineering.					
36674765	6	31	theme	Scanning	717:724	arg1	microscopy					735:744	Scanning electron microscopy	717:744	Scanning electron microscopy analysis	717:753	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	3	32	theme	cross-linking	409:421	arg1	cation					423:428	the cross-linking cation	405:428	the cross-linking cation	405:428	The concentration of the cross-linking cation failed to influence the adhesion of the pectin hydrogel to the serosa.					
36674765	7	33	theme	physiological	1018:1030	arg1	solutions					1032:1040	physiological solutions	1018:1040	physiological solutions	1018:1040	The effect of the salt composition of the adhesion medium was shown since the FeP hydrogel's adhesion to the serosa was lower in physiological solutions than in water, and adhesion in Hanks' solution was higher than in phosphate buffered saline.					
36674765	6	34	theme	internal	830:837	arg1	structure					839:847	its denser internal structure	819:847	its denser internal structure	819:847	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	3	35	theme	cation	423:428	arg1	concentration					388:400	The concentration	384:400	The concentration of the cross-linking cation	384:428	The concentration of the cross-linking cation failed to influence the adhesion of the pectin hydrogel to the serosa.					
36674765	0	36	theme	Hydrogels	28:36	arg1	Adhesion					8:15	Serosal Adhesion	0:15	Serosal Adhesion Ex Vivo of Hydrogels Prepared from Apple Pectin	0:63	Serosal Adhesion Ex Vivo of Hydrogels Prepared from Apple Pectin Cross-Linked with Fe3+ Ions.					
36674765	4	37	theme	mechanical	505:514	arg1	properties					516:525	The mechanical properties	501:525	The mechanical properties	501:525	The mechanical properties and surface microrelief of the pectin hydrogel were influenced by the type and concentration of the cross-linking cations.					
36674765	6	38	theme	denser	823:828	arg1	structure					839:847	its denser internal structure	819:847	its denser internal structure	819:847	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	4	39	dep	type	597:600	arg1	the					593:595	the	593:595	the	593:595	The mechanical properties and surface microrelief of the pectin hydrogel were influenced by the type and concentration of the cross-linking cations.					
36674765	9	40	theme	incubation	1382:1391	arg1	pH					1372:1373	the pH	1368:1373	the pH of the incubation	1368:1391	Pre-incubation in Hanks' solution for 24 h significantly reduced the adhesion of the FeP hydrogel to the serosa, regardless of the pH of the incubation.					
36674765	5	41	theme	harder	670:675	arg1	gel					694:696	a harder and more elastic gel	668:696	a harder and more elastic gel than Ca2+ cations	668:714	Fe3+ cations form a harder and more elastic gel than Ca2+ cations.					
36674765	2	42	theme	Zn2+	360:363	arg1	cations					375:381	Ca2+, Zn2+, and Al3+ cations	354:381	Ca2+, Zn2+, and Al3+ cations	354:381	The adhesivity of the FeP hydrogel, which was cross-linked by Fe3+ cations, exceeded that of hydrogels cross-linked by Ca2+, Zn2+, and Al3+ cations.					
36674765	10	43	theme	hydrogel	1544:1551	arg1	advantages					1522:1531	advantages	1522:1531	advantages of the FeP hydrogel	1522:1551	Thus, serosal adhesion combined with excellent stability and mechanical properties in physiological environments appeared to be advantages of the FeP hydrogel, demonstrating it to be a promising bioadhesive for tissue engineering.					
36674765	5	44	theme	Ca2+	703:706	arg1	cations					708:714	Ca2+ cations	703:714	Ca2+ cations	703:714	Fe3+ cations form a harder and more elastic gel than Ca2+ cations.					
36674765	8	45	theme	FeP	1227:1229	arg1	hydrogel					1231:1238	the FeP hydrogel	1223:1238	the FeP hydrogel	1223:1238	Serum proteins and peritoneal leukocytes did not interfere with the serosal adhesion of the FeP hydrogel.					
36674765	8	46	theme	peritoneal	1154:1163	arg1	leukocytes					1165:1174	peritoneal leukocytes	1154:1174	peritoneal leukocytes	1154:1174	Serum proteins and peritoneal leukocytes did not interfere with the serosal adhesion of the FeP hydrogel.					
36674765	7	47	theme	salt	907:910	arg1	composition					912:922	the salt composition	903:922	the salt composition of the adhesion medium	903:945	The effect of the salt composition of the adhesion medium was shown since the FeP hydrogel's adhesion to the serosa was lower in physiological solutions than in water, and adhesion in Hanks' solution was higher than in phosphate buffered saline.					
36674765	10	48	theme	excellent	1431:1439	arg1	stability					1441:1449	excellent stability	1431:1449	excellent stability	1431:1449	Thus, serosal adhesion combined with excellent stability and mechanical properties in physiological environments appeared to be advantages of the FeP hydrogel, demonstrating it to be a promising bioadhesive for tissue engineering.					
36674765	6	49	theme	hydrogel	806:813	arg1	pattern					791:797	the characteristic surface pattern	764:797	the characteristic surface pattern of FeP hydrogel and its denser internal structure	764:847	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	7	50	theme	FeP	967:969	arg1	hydrogel					971:978	the FeP hydrogel's	963:980	the FeP hydrogel's adhesion to the serosa	963:1003	The effect of the salt composition of the adhesion medium was shown since the FeP hydrogel's adhesion to the serosa was lower in physiological solutions than in water, and adhesion in Hanks' solution was higher than in phosphate buffered saline.					
36674765	10	51	theme	physiological	1480:1492	arg1	environments					1494:1505	physiological environments	1480:1505	physiological environments	1480:1505	Thus, serosal adhesion combined with excellent stability and mechanical properties in physiological environments appeared to be advantages of the FeP hydrogel, demonstrating it to be a promising bioadhesive for tissue engineering.					
36674765	6	52	theme	FeP	802:804	arg1	hydrogel					806:813	FeP hydrogel	802:813	FeP hydrogel	802:813	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	4	53	theme	cross-linking	627:639	arg1	cations					641:647	the cross-linking cations	623:647	the cross-linking cations	623:647	The mechanical properties and surface microrelief of the pectin hydrogel were influenced by the type and concentration of the cross-linking cations.					
36674765	2	54	theme	Fe3+	297:300	arg1	cations					302:308	Fe3+ cations	297:308	Fe3+ cations	297:308	The adhesivity of the FeP hydrogel, which was cross-linked by Fe3+ cations, exceeded that of hydrogels cross-linked by Ca2+, Zn2+, and Al3+ cations.					
36674765	4	55	theme	pectin	558:563	arg1	hydrogel					565:572	the pectin hydrogel	554:572	the pectin hydrogel	554:572	The mechanical properties and surface microrelief of the pectin hydrogel were influenced by the type and concentration of the cross-linking cations.					
36674765	5	56	theme	Fe3+	650:653	arg1	cations					655:661	Fe3+ cations	650:661	Fe3+ cations	650:661	Fe3+ cations form a harder and more elastic gel than Ca2+ cations.					
36674765	3	57	theme	pectin	470:475	arg1	hydrogel					477:484	the pectin hydrogel	466:484	the pectin hydrogel	466:484	The concentration of the cross-linking cation failed to influence the adhesion of the pectin hydrogel to the serosa.					
36674765	1	58	theme	intestinal	208:217	arg1	serosa					219:224	rat intestinal serosa	204:224	rat intestinal serosa	204:224	The study aims to investigate the adhesion of a hydrogel made of cross-linked low-methyl esterified pectin to rat intestinal serosa ex vivo.					
36674765	1	59	theme	low-methyl	172:181	arg1	pectin					194:199	cross-linked low-methyl esterified pectin	159:199	cross-linked low-methyl esterified pectin	159:199	The study aims to investigate the adhesion of a hydrogel made of cross-linked low-methyl esterified pectin to rat intestinal serosa ex vivo.					
36674765	2	60	theme	Al3+	370:373	arg1	cations					375:381	Ca2+, Zn2+, and Al3+ cations	354:381	Ca2+, Zn2+, and Al3+ cations	354:381	The adhesivity of the FeP hydrogel, which was cross-linked by Fe3+ cations, exceeded that of hydrogels cross-linked by Ca2+, Zn2+, and Al3+ cations.					
36674765	4	61	theme	cations	641:647	arg1	concentration					606:618	concentration	606:618	concentration	606:618	The mechanical properties and surface microrelief of the pectin hydrogel were influenced by the type and concentration of the cross-linking cations.					
36674765	4	61	theme	cations	641:647	arg1	type					597:600	type	597:600	type	597:600	The mechanical properties and surface microrelief of the pectin hydrogel were influenced by the type and concentration of the cross-linking cations.					
36674765	6	62	link	cross-linked	866:877	arg1	hydrogel					879:886	Ca2+ cross-linked hydrogel	861:886	Ca2+ cross-linked hydrogel	861:886	Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel.					
36674765	9	63	theme	FeP	1326:1328	arg1	hydrogel					1330:1337	the FeP hydrogel	1322:1337	the FeP hydrogel	1322:1337	Pre-incubation in Hanks' solution for 24 h significantly reduced the adhesion of the FeP hydrogel to the serosa, regardless of the pH of the incubation.					
36674765	0	64	theme	Apple	52:56	arg1	Pectin					58:63	Apple Pectin	52:63	Apple Pectin	52:63	Serosal Adhesion Ex Vivo of Hydrogels Prepared from Apple Pectin Cross-Linked with Fe3+ Ions.					
36674765	7	65	theme	buffered	1118:1125	arg1	saline					1127:1132	phosphate buffered saline	1108:1132	phosphate buffered saline	1108:1132	The effect of the salt composition of the adhesion medium was shown since the FeP hydrogel's adhesion to the serosa was lower in physiological solutions than in water, and adhesion in Hanks' solution was higher than in phosphate buffered saline.					
36674765	9	66	theme	hydrogel	1330:1337	arg1	adhesion					1310:1317	the adhesion	1306:1317	the adhesion of the FeP hydrogel to the serosa	1306:1351	Pre-incubation in Hanks' solution for 24 h significantly reduced the adhesion of the FeP hydrogel to the serosa, regardless of the pH of the incubation.					
36674765	7	67	theme	medium	940:945	arg1	composition					912:922	the salt composition	903:922	the salt composition of the adhesion medium	903:945	The effect of the salt composition of the adhesion medium was shown since the FeP hydrogel's adhesion to the serosa was lower in physiological solutions than in water, and adhesion in Hanks' solution was higher than in phosphate buffered saline.					
36674765	1	68	theme	rat	204:206	arg1	serosa					219:224	rat intestinal serosa	204:224	rat intestinal serosa	204:224	The study aims to investigate the adhesion of a hydrogel made of cross-linked low-methyl esterified pectin to rat intestinal serosa ex vivo.					
36674765	4	69	theme	hydrogel	565:572	arg1	microrelief					539:549	surface microrelief	531:549	surface microrelief	531:549	The mechanical properties and surface microrelief of the pectin hydrogel were influenced by the type and concentration of the cross-linking cations.					
36674765	4	69	theme	hydrogel	565:572	arg1	properties					516:525	The mechanical properties	501:525	The mechanical properties	501:525	The mechanical properties and surface microrelief of the pectin hydrogel were influenced by the type and concentration of the cross-linking cations.					
36813025	11	0	dep	associated	1431:1440	arg1	2.20					1564:1567	2.20	1564:1567	2.20	1564:1567	Higher levels of 2'FL were associated with greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65]) and length (β=1.22, [0.25, 2.20]) in children born to secretor mothers, but not body composition measures.					
36813025	6	1	theme	Maternal	748:755	arg1	status					766:771	Maternal secretor status	748:771	Maternal secretor status (n=221 secretors)	748:789	Maternal secretor status (n=221 secretors) was determined from 2'-fucosyllactose (2'FL) concentration.					
36813025	6	1	theme	Maternal	748:755	arg1	secretors					780:788	n=221 secretors	774:788	n=221 secretors	774:788	Maternal secretor status (n=221 secretors) was determined from 2'-fucosyllactose (2'FL) concentration.					
36813025	2	2	theme	weeks	329:333	arg1	postpartum					335:344	6 weeks postpartum	327:344	6 weeks postpartum	327:344	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	14	3	theme	different	2095:2103	arg1	HMOs					2105:2108	largely different HMOs	2087:2108	largely different HMOs associating with anthropometry from 12 months to 4 years of age	2087:2172	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	14	4	theme	Milk	1914:1917	arg1	composition					1923:1933	CONCLUSIONS Milk HMO composition	1902:1933	CONCLUSIONS Milk HMO composition at 6 weeks postpartum	1902:1955	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	8	5	theme	status	1082:1087	arg1	measure					1102:1108	each HMO measure	1093:1108	each HMO measure with change from birth for each z-score	1093:1148	We investigated associations of secretor status and each HMO measure with change from birth for each z-score using linear mixed-effects models.					
36813025	8	5	theme	status	1082:1087	arg1	associations					1057:1068	associations	1057:1068	associations of secretor status	1057:1087	We investigated associations of secretor status and each HMO measure with change from birth for each z-score using linear mixed-effects models.					
36813025	9	6	dep	RESULTS	1185:1191	arg1	associated					1226:1235	associated	1226:1235	was not associated with anthropometric z-scores up to 4 years of age	1218:1285	RESULTS Maternal secretor status was not associated with anthropometric z-scores up to 4 years of age.					
36813025	14	7	from	manner	2074:2079	arg1	measures					1984:1991	several measures	1976:1991	several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner	1976:2079	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	12	8	dep	associated	1678:1687	arg1	0.53					1759:1762	0.53	1759:1762	0.53	1759:1762	Higher lacto-N-tetraose (LNT) was associated with greater weight (β=0.22, [0.02, 0.41]) and length (β=0.30, [0.07, 0.53]) among children born to non-secretor mothers.					
36813025	3	9	theme	postpartum	510:519	arg1	weeks					486:490	6 weeks [median 6.0 weeks	466:490	6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort	466:564	METHODS Milk samples were collected from 292 mothers at 6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort.					
36813025	10	10	theme	Several	1288:1294	arg1	HMOs					1296:1299	Several HMOs	1288:1299	Several HMOs	1288:1299	Several HMOs were associated with z-scores at 6 weeks and 6 months, predominantly within secretor status subgroups.					
36813025	4	11	theme	infants	574:580	arg1	milk-fed					610:617	milk-fed	610:617	milk-fed	610:617	Of the infants, 171 were exclusively human milk-fed to 3 months of age, and 127 to 6 months.					
36813025	0	12	theme	age	106:108	arg1	years					97:101	up to 4 years	89:101	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.	0:109	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.					
36813025	13	13	theme	anthropometric	1845:1858	arg1	measures					1860:1867	anthropometric measures	1845:1867	anthropometric measures at 12 months and 4 years of age	1845:1899	Several HMOs were associated with anthropometric measures at 12 months and 4 years of age.					
36813025	3	14	theme	weeks	468:472	arg1	weeks					486:490	6 weeks [median 6.0 weeks	466:490	6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort	466:564	METHODS Milk samples were collected from 292 mothers at 6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort.					
36813025	1	15	theme	human	181:185	arg1	milk					187:190	human milk	181:190	human milk	181:190	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	12	16	theme	non-secretor	1789:1800	arg1	mothers					1802:1808	non-secretor mothers	1789:1808	non-secretor mothers	1789:1808	Higher lacto-N-tetraose (LNT) was associated with greater weight (β=0.22, [0.02, 0.41]) and length (β=0.30, [0.07, 0.53]) among children born to non-secretor mothers.					
36813025	8	17	theme	mixed-effects	1163:1175	arg1	models					1177:1182	linear mixed-effects models	1156:1182	linear mixed-effects models	1156:1182	We investigated associations of secretor status and each HMO measure with change from birth for each z-score using linear mixed-effects models.					
36813025	11	18	from	increase	1470:1477	arg1	z-score					1482:1488	z-score	1482:1488	z-score per SD increase	1482:1504	Higher levels of 2'FL were associated with greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65]) and length (β=1.22, [0.25, 2.20]) in children born to secretor mothers, but not body composition measures.					
36813025	8	19	from	birth	1127:1131	arg1	change					1115:1120	change	1115:1120	change from birth	1115:1131	We investigated associations of secretor status and each HMO measure with change from birth for each z-score using linear mixed-effects models.					
36813025	10	20	from	months	1348:1353	arg1	z-scores					1322:1329	z-scores	1322:1329	z-scores at 6 weeks and 6 months	1322:1353	Several HMOs were associated with z-scores at 6 weeks and 6 months, predominantly within secretor status subgroups.					
36813025	7	21	theme	skinfold	951:958	arg1	thicknesses					960:970	summed triceps and subscapular skinfold thicknesses	920:970	thicknesses	960:970	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	22	theme	head	900:903	arg1	circumference					905:917	head circumference	900:917	head circumference	900:917	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	1	23	theme	Human	122:126	arg1	oligosaccharides					133:148	BACKGROUND Human milk oligosaccharides	111:148	BACKGROUND Human milk oligosaccharides (HMOs)	111:155	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	1	23	theme	Human	122:126	arg1	components					167:176	major components	161:176	major components of human milk that may mediate its beneficial effects on infant growth	161:247	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	1	23	theme	Human	122:126	arg1	HMOs					151:154	HMOs	151:154	HMOs	151:154	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	4	24	dep	6	650:650	arg1	to					647:648	to	647:648	to	647:648	Of the infants, 171 were exclusively human milk-fed to 3 months of age, and 127 to 6 months.					
36813025	3	25	from	postpartum	510:519	arg1	cohort					559:564	a longitudinal, population-derived cohort	524:564	a longitudinal, population-derived cohort	524:564	METHODS Milk samples were collected from 292 mothers at 6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort.					
36813025	14	26	from	postpartum	1946:1955	arg1	composition					1923:1933	CONCLUSIONS Milk HMO composition	1902:1933	CONCLUSIONS Milk HMO composition at 6 weeks postpartum	1902:1955	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	9	27	dep	z-scores	1257:1264	arg1	years					1274:1278	up to 4 years	1266:1278	anthropometric z-scores up to 4 years of age	1242:1285	RESULTS Maternal secretor status was not associated with anthropometric z-scores up to 4 years of age.					
36813025	5	28	theme	HMOs	681:684	arg1	Concentrations					660:673	Concentrations	660:673	Concentrations of 19 HMOs	660:684	Concentrations of 19 HMOs were quantified using high-performance liquid chromatography.					
36813025	12	29	theme	greater	1694:1700	arg1	weight					1702:1707	greater weight	1694:1707	greater weight (β=0.22, [0.02, 0.41])	1694:1730	Higher lacto-N-tetraose (LNT) was associated with greater weight (β=0.22, [0.02, 0.41]) and length (β=0.30, [0.07, 0.53]) among children born to non-secretor mothers.					
36813025	2	30	from	concentrations	301:314	arg1	postpartum					335:344	6 weeks postpartum	327:344	6 weeks postpartum	327:344	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	2	30	from	concentrations	301:314	arg1	anthropometry					351:363	anthropometry	351:363	anthropometry	351:363	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	2	30	from	concentrations	301:314	arg1	milk					319:322	milk	319:322	milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants	319:407	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	9	31	theme	secretor	1202:1209	arg1	status					1211:1216	Maternal secretor status	1193:1216	Maternal secretor status	1193:1216	RESULTS Maternal secretor status was not associated with anthropometric z-scores up to 4 years of age.					
36813025	3	32	from	cohort	559:564	arg1	postpartum					510:519	postpartum	510:519	postpartum	510:519	METHODS Milk samples were collected from 292 mothers at 6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort.					
36813025	14	33	theme	HMO	1919:1921	arg1	composition					1923:1933	CONCLUSIONS Milk HMO composition	1902:1933	CONCLUSIONS Milk HMO composition at 6 weeks postpartum	1902:1955	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	8	34	with	associations	1057:1068	arg1	change					1115:1120	change	1115:1120	change from birth	1115:1131	We investigated associations of secretor status and each HMO measure with change from birth for each z-score using linear mixed-effects models.					
36813025	0	35	theme	Age-dependent	0:12	arg1	associations					14:25	Age-dependent associations	0:25	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.	0:109	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.					
36813025	10	36	theme	status	1386:1391	arg1	subgroups					1393:1401	secretor status subgroups	1377:1401	secretor status subgroups	1377:1401	Several HMOs were associated with z-scores at 6 weeks and 6 months, predominantly within secretor status subgroups.					
36813025	14	37	theme	several	1976:1982	arg1	measures					1984:1991	several measures	1976:1991	several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner	1976:2079	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	2	38	theme	human	386:390	arg1	infants					401:407	human milk-fed infants	386:407	human milk-fed infants	386:407	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	9	39	theme	anthropometric	1242:1255	arg1	z-scores					1257:1264	anthropometric z-scores	1242:1264	anthropometric z-scores up to 4 years of age	1242:1285	RESULTS Maternal secretor status was not associated with anthropometric z-scores up to 4 years of age.					
36813025	12	40	theme	Higher	1644:1649	arg1	lacto-N-tetraose					1651:1666	Higher lacto-N-tetraose	1644:1666	Higher lacto-N-tetraose (LNT)	1644:1672	Higher lacto-N-tetraose (LNT) was associated with greater weight (β=0.22, [0.02, 0.41]) and length (β=0.30, [0.07, 0.53]) among children born to non-secretor mothers.					
36813025	7	41	from	months	1009:1014	arg1	circumference					905:917	head circumference	900:917	head circumference	900:917	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	41	from	months	1009:1014	arg1	thicknesses					960:970	summed triceps and subscapular skinfold thicknesses	920:970	thicknesses	960:970	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	41	from	months	1009:1014	arg1	weight					884:889	child weight	878:889	child weight	878:889	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	41	from	months	1009:1014	arg1	triceps					927:933	summed triceps and subscapular skinfold thicknesses	920:970	triceps	927:933	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	41	from	months	1009:1014	arg1	weight-for-length					977:993	weight-for-length	977:993	weight-for-length	977:993	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	41	from	months	1009:1014	arg1	length					892:897	length	892:897	length	892:897	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	13	42	from	months	1875:1880	arg1	measures					1860:1867	anthropometric measures	1845:1867	anthropometric measures at 12 months and 4 years of age	1845:1899	Several HMOs were associated with anthropometric measures at 12 months and 4 years of age.					
36813025	2	43	theme	age	379:381	arg1	years					370:374	4 years	368:374	4 years of age in human milk-fed infants	368:407	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	3	44	theme	Milk	418:421	arg1	samples					423:429	Milk samples	418:429	Milk samples	418:429	METHODS Milk samples were collected from 292 mothers at 6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort.					
36813025	0	45	theme	milk	36:39	arg1	oligosaccharides					41:56	human milk oligosaccharides	30:56	human milk oligosaccharides	30:56	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.					
36813025	14	46	theme	age	2170:2172	arg1	years					2161:2165	4 years	2159:2165	4 years of age	2159:2172	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	5	47	theme	high-performance	708:723	arg1	chromatography					732:745	high-performance liquid chromatography	708:745	high-performance liquid chromatography	708:745	Concentrations of 19 HMOs were quantified using high-performance liquid chromatography.					
36813025	3	48	dep	METHODS	410:416	arg1	collected					436:444	collected	436:444	were collected from 292 mothers at 6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort	431:564	METHODS Milk samples were collected from 292 mothers at 6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort.					
36813025	2	49	from	years	370:374	arg1	infants					401:407	human milk-fed infants	386:407	human milk-fed infants	386:407	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	14	50	dep	months	2018:2023	arg1	up					2010:2011	up	2010:2011	up	2010:2011	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	11	51	theme	body	1617:1620	arg1	measures					1634:1641	body composition measures	1617:1641	body composition measures	1617:1641	Higher levels of 2'FL were associated with greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65]) and length (β=1.22, [0.25, 2.20]) in children born to secretor mothers, but not body composition measures.					
36813025	11	52	theme	SD	1494:1495	arg1	increase					1497:1504	SD increase	1494:1504	SD increase	1494:1504	Higher levels of 2'FL were associated with greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65]) and length (β=1.22, [0.25, 2.20]) in children born to secretor mothers, but not body composition measures.					
36813025	3	53	theme	[median	474:480	arg1	weeks					486:490	6 weeks [median 6.0 weeks	466:490	6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort	466:564	METHODS Milk samples were collected from 292 mothers at 6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort.					
36813025	14	54	theme	weeks	1940:1944	arg1	postpartum					1946:1955	6 weeks postpartum	1938:1955	6 weeks postpartum	1938:1955	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	14	55	theme	age	2028:2030	arg1	months					2018:2023	6 months	2016:2023	6 months of age	2016:2030	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	11	56	theme	FL	1423:1424	arg1	levels					1411:1416	Higher levels	1404:1416	Higher levels of 2'FL	1404:1424	Higher levels of 2'FL were associated with greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65]) and length (β=1.22, [0.25, 2.20]) in children born to secretor mothers, but not body composition measures.					
36813025	1	57	theme	infant	235:240	arg1	growth					242:247	infant growth	235:247	infant growth	235:247	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	11	58	dep	weight	1455:1460	arg1	CI					1520:1521	CI	1520:1521	CI	1520:1521	Higher levels of 2'FL were associated with greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65]) and length (β=1.22, [0.25, 2.20]) in children born to secretor mothers, but not body composition measures.					
36813025	12	59	dep	lacto-N-tetraose	1651:1666	arg1	LNT					1669:1671	LNT	1669:1671	LNT	1669:1671	Higher lacto-N-tetraose (LNT) was associated with greater weight (β=0.22, [0.02, 0.41]) and length (β=0.30, [0.07, 0.53]) among children born to non-secretor mothers.					
36813025	1	60	theme	major	161:165	arg1	oligosaccharides					133:148	BACKGROUND Human milk oligosaccharides	111:148	BACKGROUND Human milk oligosaccharides (HMOs)	111:155	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	1	60	theme	major	161:165	arg1	components					167:176	major components	161:176	major components of human milk that may mediate its beneficial effects on infant growth	161:247	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	6	61	theme	2'-fucosyllactose	811:827	arg1	concentration					836:848	2'-fucosyllactose (2'FL) concentration	811:848	2'-fucosyllactose (2'FL) concentration	811:848	Maternal secretor status (n=221 secretors) was determined from 2'-fucosyllactose (2'FL) concentration.					
36813025	2	62	from	postpartum	335:344	arg1	milk					319:322	milk	319:322	milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants	319:407	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	2	62	from	postpartum	335:344	arg1	concentrations					301:314	HMO concentrations	297:314	HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants	297:407	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	14	63	theme	CONCLUSIONS	1902:1912	arg1	composition					1923:1933	CONCLUSIONS Milk HMO composition	1902:1933	CONCLUSIONS Milk HMO composition at 6 weeks postpartum	1902:1955	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	11	64	theme	greater	1447:1453	arg1	weight					1455:1460	greater weight	1447:1460	greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65])	1447:1535	Higher levels of 2'FL were associated with greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65]) and length (β=1.22, [0.25, 2.20]) in children born to secretor mothers, but not body composition measures.					
36813025	3	65	dep	longitudinal	526:537	arg1	population-derived					540:557	population-derived	540:557	population-derived	540:557	METHODS Milk samples were collected from 292 mothers at 6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort.					
36813025	8	66	theme	secretor	1073:1080	arg1	status					1082:1087	secretor status	1073:1087	secretor status	1073:1087	We investigated associations of secretor status and each HMO measure with change from birth for each z-score using linear mixed-effects models.					
36813025	11	67	theme	secretor	1591:1598	arg1	mothers					1600:1606	secretor mothers	1591:1606	secretor mothers	1591:1606	Higher levels of 2'FL were associated with greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65]) and length (β=1.22, [0.25, 2.20]) in children born to secretor mothers, but not body composition measures.					
36813025	14	68	theme	status-specific	2058:2072	arg1	manner					2074:2079	a potentially secretor status-specific manner	2035:2079	a potentially secretor status-specific manner	2035:2079	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	2	69	theme	HMO	297:299	arg1	concentrations					301:314	HMO concentrations	297:314	HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants	297:407	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	10	70	from	weeks	1336:1340	arg1	z-scores					1322:1329	z-scores	1322:1329	z-scores at 6 weeks and 6 months	1322:1353	Several HMOs were associated with z-scores at 6 weeks and 6 months, predominantly within secretor status subgroups.					
36813025	1	71	from	effects	224:230	arg1	growth					242:247	infant growth	235:247	infant growth	235:247	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	3	72	theme	longitudinal	526:537	arg1	cohort					559:564	a longitudinal, population-derived cohort	524:564	a longitudinal, population-derived cohort	524:564	METHODS Milk samples were collected from 292 mothers at 6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort.					
36813025	1	73	theme	milk	187:190	arg1	oligosaccharides					133:148	BACKGROUND Human milk oligosaccharides	111:148	BACKGROUND Human milk oligosaccharides (HMOs)	111:155	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	1	73	theme	milk	187:190	arg1	components					167:176	major components	161:176	major components of human milk that may mediate its beneficial effects on infant growth	161:247	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	8	74	theme	HMO	1098:1100	arg1	measure					1102:1108	each HMO measure	1093:1108	each HMO measure with change from birth for each z-score	1093:1148	We investigated associations of secretor status and each HMO measure with change from birth for each z-score using linear mixed-effects models.					
36813025	14	75	from	months	2149:2154	arg1	anthropometry					2127:2139	anthropometry	2127:2139	anthropometry from 12 months to 4 years of age	2127:2172	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	8	76	theme	linear	1156:1161	arg1	models					1177:1182	linear mixed-effects models	1156:1182	linear mixed-effects models	1156:1182	We investigated associations of secretor status and each HMO measure with change from birth for each z-score using linear mixed-effects models.					
36813025	12	77	dep	weight	1702:1707	arg1	β=0.22					1710:1715	β=0.22	1710:1715	β=0.22	1710:1715	Higher lacto-N-tetraose (LNT) was associated with greater weight (β=0.22, [0.02, 0.41]) and length (β=0.30, [0.07, 0.53]) among children born to non-secretor mothers.					
36813025	3	78	dep	weeks	486:490	arg1	range					493:497	range 3.3, 11.1	493:507	range 3.3, 11.1	493:507	METHODS Milk samples were collected from 292 mothers at 6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort.					
36813025	13	79	theme	Several	1811:1817	arg1	HMOs					1819:1822	Several HMOs	1811:1822	Several HMOs	1811:1822	Several HMOs were associated with anthropometric measures at 12 months and 4 years of age.					
36813025	4	80	theme	age	634:636	arg1	months					624:629	3 months	622:629	3 months of age	622:636	Of the infants, 171 were exclusively human milk-fed to 3 months of age, and 127 to 6 months.					
36813025	4	80	theme	age	634:636	arg1	months					652:657	127 to 6 months	643:657	127 to 6 months	643:657	Of the infants, 171 were exclusively human milk-fed to 3 months of age, and 127 to 6 months.					
36813025	7	81	theme	subscapular	939:949	arg1	thicknesses					960:970	summed triceps and subscapular skinfold thicknesses	920:970	thicknesses	960:970	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	82	theme	summed	920:925	arg1	triceps					927:933	summed triceps and subscapular skinfold thicknesses	920:970	triceps	927:933	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	1	83	theme	BACKGROUND	111:120	arg1	oligosaccharides					133:148	BACKGROUND Human milk oligosaccharides	111:148	BACKGROUND Human milk oligosaccharides (HMOs)	111:155	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	1	83	theme	BACKGROUND	111:120	arg1	components					167:176	major components	161:176	major components of human milk that may mediate its beneficial effects on infant growth	161:247	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	1	83	theme	BACKGROUND	111:120	arg1	HMOs					151:154	HMOs	151:154	HMOs	151:154	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	1	84	theme	milk	128:131	arg1	oligosaccharides					133:148	BACKGROUND Human milk oligosaccharides	111:148	BACKGROUND Human milk oligosaccharides (HMOs)	111:155	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	1	84	theme	milk	128:131	arg1	components					167:176	major components	161:176	major components of human milk that may mediate its beneficial effects on infant growth	161:247	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	1	84	theme	milk	128:131	arg1	HMOs					151:154	HMOs	151:154	HMOs	151:154	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36813025	8	85	with	measure	1102:1108	arg1	change					1115:1120	change	1115:1120	change from birth	1115:1131	We investigated associations of secretor status and each HMO measure with change from birth for each z-score using linear mixed-effects models.					
36813025	11	86	theme	Higher	1404:1409	arg1	levels					1411:1416	Higher levels	1404:1416	Higher levels of 2'FL	1404:1424	Higher levels of 2'FL were associated with greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65]) and length (β=1.22, [0.25, 2.20]) in children born to secretor mothers, but not body composition measures.					
36813025	7	87	from	years	1034:1038	arg1	circumference					905:917	head circumference	900:917	head circumference	900:917	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	87	from	years	1034:1038	arg1	thicknesses					960:970	summed triceps and subscapular skinfold thicknesses	920:970	thicknesses	960:970	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	87	from	years	1034:1038	arg1	weight					884:889	child weight	878:889	child weight	878:889	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	87	from	years	1034:1038	arg1	triceps					927:933	summed triceps and subscapular skinfold thicknesses	920:970	triceps	927:933	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	87	from	years	1034:1038	arg1	weight-for-length					977:993	weight-for-length	977:993	weight-for-length	977:993	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	87	from	years	1034:1038	arg1	length					892:897	length	892:897	length	892:897	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	9	88	theme	Maternal	1193:1200	arg1	status					1211:1216	Maternal secretor status	1193:1216	Maternal secretor status	1193:1216	RESULTS Maternal secretor status was not associated with anthropometric z-scores up to 4 years of age.					
36813025	9	89	dep	4	1272:1272	arg1	to					1269:1270	to	1269:1270	to	1269:1270	RESULTS Maternal secretor status was not associated with anthropometric z-scores up to 4 years of age.					
36813025	3	90	theme	292	451:453	arg1	mothers					455:461	292 mothers	451:461	292 mothers	451:461	METHODS Milk samples were collected from 292 mothers at 6 weeks [median 6.0 weeks; range 3.3, 11.1] postpartum in a longitudinal, population-derived cohort.					
36813025	7	91	theme	child	878:882	arg1	weight					884:889	child weight	878:889	child weight	878:889	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	0	92	dep	4	95:95	arg1	to					92:93	to	92:93	to	92:93	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.					
36813025	13	93	from	years	1888:1892	arg1	measures					1860:1867	anthropometric measures	1845:1867	anthropometric measures at 12 months and 4 years of age	1845:1899	Several HMOs were associated with anthropometric measures at 12 months and 4 years of age.					
36813025	13	94	theme	age	1897:1899	arg1	months					1875:1880	12 months	1872:1880	12 months	1872:1880	Several HMOs were associated with anthropometric measures at 12 months and 4 years of age.					
36813025	13	94	theme	age	1897:1899	arg1	years					1888:1892	4 years	1886:1892	4 years	1886:1892	Several HMOs were associated with anthropometric measures at 12 months and 4 years of age.					
36813025	14	95	theme	anthropometry	1996:2008	arg1	measures					1984:1991	several measures	1976:1991	several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner	1976:2079	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	5	96	theme	liquid	725:730	arg1	chromatography					732:745	high-performance liquid chromatography	708:745	high-performance liquid chromatography	708:745	Concentrations of 19 HMOs were quantified using high-performance liquid chromatography.					
36813025	7	97	from	weeks	1000:1004	arg1	circumference					905:917	head circumference	900:917	head circumference	900:917	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	97	from	weeks	1000:1004	arg1	thicknesses					960:970	summed triceps and subscapular skinfold thicknesses	920:970	thicknesses	960:970	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	97	from	weeks	1000:1004	arg1	weight					884:889	child weight	878:889	child weight	878:889	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	97	from	weeks	1000:1004	arg1	triceps					927:933	summed triceps and subscapular skinfold thicknesses	920:970	triceps	927:933	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	97	from	weeks	1000:1004	arg1	weight-for-length					977:993	weight-for-length	977:993	weight-for-length	977:993	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	97	from	weeks	1000:1004	arg1	length					892:897	length	892:897	length	892:897	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	0	98	theme	human	30:34	arg1	oligosaccharides					41:56	human milk oligosaccharides	30:56	human milk oligosaccharides	30:56	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.					
36813025	2	99	theme	milk-fed	392:399	arg1	infants					401:407	human milk-fed infants	386:407	human milk-fed infants	386:407	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	10	100	theme	secretor	1377:1384	arg1	status					1386:1391	secretor status	1377:1391	secretor status subgroups	1377:1401	Several HMOs were associated with z-scores at 6 weeks and 6 months, predominantly within secretor status subgroups.					
36813025	0	101	theme	oligosaccharides	41:56	arg1	associations					14:25	Age-dependent associations	0:25	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.	0:109	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.					
36813025	14	102	from	measures	1984:1991	arg1	manner					2074:2079	a potentially secretor status-specific manner	2035:2079	a potentially secretor status-specific manner	2035:2079	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	14	103	from	anthropometry	1996:2008	arg1	manner					2074:2079	a potentially secretor status-specific manner	2035:2079	a potentially secretor status-specific manner	2035:2079	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	14	104	with	HMOs	2105:2108	arg1	anthropometry					2127:2139	anthropometry	2127:2139	anthropometry from 12 months to 4 years of age	2127:2172	CONCLUSIONS Milk HMO composition at 6 weeks postpartum is associated with several measures of anthropometry up to 6 months of age in a potentially secretor status-specific manner, with largely different HMOs associating with anthropometry from 12 months to 4 years of age.					
36813025	0	105	theme	body	63:66	arg1	size					68:71	body size	63:71	body size	63:71	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.					
36813025	2	106	from	anthropometry	351:363	arg1	milk					319:322	milk	319:322	milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants	319:407	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	2	106	from	anthropometry	351:363	arg1	concentrations					301:314	HMO concentrations	297:314	HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants	297:407	OBJECTIVE To investigate relationships between HMO concentrations in milk at 6 weeks postpartum, and anthropometry to 4 years of age in human milk-fed infants.					
36813025	7	107	from	months	1020:1025	arg1	circumference					905:917	head circumference	900:917	head circumference	900:917	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	107	from	months	1020:1025	arg1	thicknesses					960:970	summed triceps and subscapular skinfold thicknesses	920:970	thicknesses	960:970	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	107	from	months	1020:1025	arg1	weight					884:889	child weight	878:889	child weight	878:889	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	107	from	months	1020:1025	arg1	triceps					927:933	summed triceps and subscapular skinfold thicknesses	920:970	triceps	927:933	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	107	from	months	1020:1025	arg1	weight-for-length					977:993	weight-for-length	977:993	weight-for-length	977:993	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	7	107	from	months	1020:1025	arg1	length					892:897	length	892:897	length	892:897	We calculated z-scores for child weight, length, head circumference, summed triceps and subscapular skinfold thicknesses, and weight-for-length at 6 weeks, 6 months, 12 months, and 4 years.					
36813025	0	108	with	associations	14:25	arg1	composition					77:87	composition	77:87	composition	77:87	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.					
36813025	0	108	with	associations	14:25	arg1	size					68:71	body size	63:71	body size	63:71	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.					
36813025	11	109	dep	CI	1520:1521	arg1	increase					1470:1477	β=0.91 increase	1463:1477	β=0.91 increase in z-score per SD increase log-2'FL	1463:1513	Higher levels of 2'FL were associated with greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65]) and length (β=1.22, [0.25, 2.20]) in children born to secretor mothers, but not body composition measures.					
36813025	11	109	dep	CI	1520:1521	arg1	%					1518:1518	95%	1516:1518	95%	1516:1518	Higher levels of 2'FL were associated with greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65]) and length (β=1.22, [0.25, 2.20]) in children born to secretor mothers, but not body composition measures.					
36813025	11	110	theme	composition	1622:1632	arg1	measures					1634:1641	body composition measures	1617:1641	body composition measures	1617:1641	Higher levels of 2'FL were associated with greater weight (β=0.91 increase in z-score per SD increase log-2'FL, 95% CI [0.17, 1.65]) and length (β=1.22, [0.25, 2.20]) in children born to secretor mothers, but not body composition measures.					
36813025	9	111	theme	age	1283:1285	arg1	years					1274:1278	up to 4 years	1266:1278	anthropometric z-scores up to 4 years of age	1242:1285	RESULTS Maternal secretor status was not associated with anthropometric z-scores up to 4 years of age.					
36813025	6	112	theme	secretor	757:764	arg1	status					766:771	Maternal secretor status	748:771	Maternal secretor status (n=221 secretors)	748:789	Maternal secretor status (n=221 secretors) was determined from 2'-fucosyllactose (2'FL) concentration.					
36813025	6	112	theme	secretor	757:764	arg1	secretors					780:788	n=221 secretors	774:788	n=221 secretors	774:788	Maternal secretor status (n=221 secretors) was determined from 2'-fucosyllactose (2'FL) concentration.					
36813025	0	113	dep	associations	14:25	arg1	years					97:101	up to 4 years	89:101	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.	0:109	Age-dependent associations of human milk oligosaccharides with body size and composition up to 4 years of age.					
36813025	1	114	theme	beneficial	213:222	arg1	effects					224:230	its beneficial effects	209:230	its beneficial effects on infant growth	209:247	BACKGROUND Human milk oligosaccharides (HMOs) are major components of human milk that may mediate its beneficial effects on infant growth.					
36736264	6	0	theme	[microglial	1107:1117	arg1	cells					1119:1123	neuroinflammation [microglial cells	1089:1123	neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)]	1089:1165	Biological response in terms of antioxidant and anti-inflammatory activity was analyzed on an in vitro model of neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)].					
36736264	11	1	theme	@	1782:1782	arg1	PLA					1783:1785	EU@PLA	1780:1785	EU@PLA	1780:1785	Overall, our results show that EU@PLA represents an innovative and effective strategy for the control of inflammatory response in central nervous system.					
36736264	7	2	theme	toll-like	1228:1236	arg1	TLR-4					1250:1254	TLR-4	1250:1254	TLR-4	1250:1254	Cell morphology and EU@PLA mechanism of action, in terms of toll-like receptor-4 (TLR-4) involvement were assessed.					
36736264	7	2	theme	toll-like	1228:1236	arg1	receptor-4					1238:1247	toll-like receptor-4	1228:1247	toll-like receptor-4 (TLR-4) involvement	1228:1267	Cell morphology and EU@PLA mechanism of action, in terms of toll-like receptor-4 (TLR-4) involvement were assessed.					
36736264	3	3	theme	@	507:507	arg1	PLA					508:510	EU@PLA	505:510	EU@PLA	505:510	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	3	3	theme	@	507:507	arg1	poly					486:489	eumelanin-coated poly	469:489	eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers	469:531	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	3	4	theme	in	536:537	arg1	model					545:549	in vitro model	536:549	in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation	536:647	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	5	5	theme	electrospinning	860:874	arg1	processes					921:929	electrospinning, spin coating and solid-state polymerization processes	860:929	electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere)	860:974	Eumelanin decorated PLA electrospun substrates were produced combining electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere).					
36736264	0	6	theme	injury	95:100	arg1	treatment					102:110	spinal cord injury treatment	83:110	spinal cord injury treatment	83:110	Eumelanin decorated poly(lactic acid) electrospun substrates as a new strategy for spinal cord injury treatment.					
36736264	6	7	theme	neuroinflammation	1089:1105	arg1	cells					1119:1123	neuroinflammation [microglial cells	1089:1123	neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)]	1089:1165	Biological response in terms of antioxidant and anti-inflammatory activity was analyzed on an in vitro model of neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)].					
36736264	5	8	theme	PLA	809:811	arg1	substrates					825:834	Eumelanin decorated PLA electrospun substrates	789:834	Eumelanin decorated PLA electrospun substrates	789:834	Eumelanin decorated PLA electrospun substrates were produced combining electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere).					
36736264	7	9	from	mechanism	1195:1203	arg1	terms					1219:1223	terms	1219:1223	terms of toll-like receptor-4 (TLR-4) involvement	1219:1267	Cell morphology and EU@PLA mechanism of action, in terms of toll-like receptor-4 (TLR-4) involvement were assessed.					
36736264	11	10	theme	innovative	1801:1810	arg1	strategy					1826:1833	an innovative and effective strategy	1798:1833	an innovative and effective strategy for the control of inflammatory response in central nervous system	1798:1900	Overall, our results show that EU@PLA represents an innovative and effective strategy for the control of inflammatory response in central nervous system.					
36736264	2	11	contain	possess	377:383	arg1	they					372:375	they	372:375	they	372:375	Natural and synthetic melanins represent an effective tool to treat neuroinflammation because they possess immunomodulatory properties.					
36736264	2	11	contain	possess	377:383	arg2	properties					402:411	immunomodulatory properties	385:411	immunomodulatory properties	385:411	Natural and synthetic melanins represent an effective tool to treat neuroinflammation because they possess immunomodulatory properties.					
36736264	3	12	theme	neuroinflammation	554:570	arg1	model					545:549	in vitro model	536:549	in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation	536:647	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	10	13	theme	expression	1638:1647	arg1	analysis					1649:1656	Protein expression analysis	1630:1656	Protein expression analysis	1630:1656	Protein expression analysis revealed a decreased in TLR-4 production induced by LPS stimulation in presence of EU@PLA.					
36736264	11	14	theme	inflammatory	1854:1865	arg1	response					1867:1874	inflammatory response	1854:1874	inflammatory response	1854:1874	Overall, our results show that EU@PLA represents an innovative and effective strategy for the control of inflammatory response in central nervous system.					
36736264	5	15	theme	polymerization	906:919	arg1	processes					921:929	electrospinning, spin coating and solid-state polymerization processes	860:929	electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere)	860:974	Eumelanin decorated PLA electrospun substrates were produced combining electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere).					
36736264	11	16	from	control	1843:1849	arg1	system					1895:1900	central nervous system	1879:1900	central nervous system	1879:1900	Overall, our results show that EU@PLA represents an innovative and effective strategy for the control of inflammatory response in central nervous system.					
36736264	4	17	theme	physical	688:695	arg1	cues					697:700	physical cues	688:700	physical cues	688:700	Aligned fibers were chosen to provide physical cues to guide axonal growth in a specific direction thus restoring the synaptic connection.					
36736264	8	18	theme	species	1358:1364	arg1	expression					1438:1447	reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression	1342:1447	reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression	1342:1447	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	3	19	theme	cord	590:593	arg1	injury					595:600	spinal cord injury	583:600	spinal cord injury in terms of inflammatory mediators' modulation	583:647	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	1	20	theme	Spinal	113:118	arg1	injury					125:130	Spinal cord injury	113:130	Spinal cord injury (SCI)	113:136	Spinal cord injury (SCI) is characterized by neuroinflammatory processes that are marked by an uncontrolled activation of microglia, which directly damages neurons.					
36736264	1	20	theme	Spinal	113:118	arg1	SCI					133:135	SCI	133:135	SCI	133:135	Spinal cord injury (SCI) is characterized by neuroinflammatory processes that are marked by an uncontrolled activation of microglia, which directly damages neurons.					
36736264	6	21	theme	anti-inflammatory	1025:1041	arg1	activity					1043:1050	antioxidant and anti-inflammatory activity	1009:1050	antioxidant and anti-inflammatory activity	1009:1050	Biological response in terms of antioxidant and anti-inflammatory activity was analyzed on an in vitro model of neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)].					
36736264	8	22	theme	EU	1306:1307	arg1	fibers					1313:1318	EU@PLA fibers	1306:1318	EU@PLA fibers	1306:1318	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	5	23	theme	Eumelanin	789:797	arg1	substrates					825:834	Eumelanin decorated PLA electrospun substrates	789:834	Eumelanin decorated PLA electrospun substrates	789:834	Eumelanin decorated PLA electrospun substrates were produced combining electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere).					
36736264	5	24	theme	spin	877:880	arg1	coating					882:888	spin coating	877:888	spin coating	877:888	Eumelanin decorated PLA electrospun substrates were produced combining electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere).					
36736264	6	25	theme	antioxidant	1009:1019	arg1	activity					1043:1050	antioxidant and anti-inflammatory activity	1009:1050	antioxidant and anti-inflammatory activity	1009:1050	Biological response in terms of antioxidant and anti-inflammatory activity was analyzed on an in vitro model of neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)].					
36736264	8	26	theme	PLA	1309:1311	arg1	fibers					1313:1318	EU@PLA fibers	1306:1318	EU@PLA fibers	1306:1318	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	3	27	dep	in	536:537	arg1	vitro					539:543	vitro	539:543	vitro	539:543	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	8	28	theme	interleukin	1481:1491	arg1	secretion					1502:1510	interleukin 6 (IL-6) secretion	1481:1510	interleukin 6 (IL-6) secretion	1481:1510	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	5	29	dep	processes	921:929	arg1	coupling					942:949	oxidative coupling	932:949	oxidative coupling under oxygen atmosphere	932:973	Eumelanin decorated PLA electrospun substrates were produced combining electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere).					
36736264	7	30	theme	action	1208:1213	arg1	mechanism					1195:1203	EU@PLA mechanism	1188:1203	EU@PLA mechanism	1188:1203	Cell morphology and EU@PLA mechanism of action, in terms of toll-like receptor-4 (TLR-4) involvement were assessed.					
36736264	7	30	theme	action	1208:1213	arg1	morphology					1173:1182	Cell morphology	1168:1182	Cell morphology	1168:1182	Cell morphology and EU@PLA mechanism of action, in terms of toll-like receptor-4 (TLR-4) involvement were assessed.					
36736264	4	31	theme	specific	730:737	arg1	direction					739:747	a specific direction	728:747	a specific direction	728:747	Aligned fibers were chosen to provide physical cues to guide axonal growth in a specific direction thus restoring the synaptic connection.					
36736264	2	32	theme	immunomodulatory	385:400	arg1	properties					402:411	immunomodulatory properties	385:411	immunomodulatory properties	385:411	Natural and synthetic melanins represent an effective tool to treat neuroinflammation because they possess immunomodulatory properties.					
36736264	0	33	theme	spinal	83:88	arg1	injury					95:100	spinal cord injury	83:100	spinal cord injury treatment	83:110	Eumelanin decorated poly(lactic acid) electrospun substrates as a new strategy for spinal cord injury treatment.					
36736264	8	34	theme	reactive	1342:1349	arg1	kappa-light-chain-enhancer					1382:1407	nuclear factor kappa-light-chain-enhancer	1367:1407	nuclear factor kappa-light-chain-enhancer of activated B cells	1367:1428	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	8	34	theme	reactive	1342:1349	arg1	NF-кB					1431:1435	NF-кB	1431:1435	NF-кB	1431:1435	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	8	34	theme	reactive	1342:1349	arg1	species					1358:1364	reactive oxygen species	1342:1364	reactive oxygen species	1342:1364	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	7	35	theme	@	1190:1190	arg1	mechanism					1195:1203	EU@PLA mechanism	1188:1203	EU@PLA mechanism	1188:1203	Cell morphology and EU@PLA mechanism of action, in terms of toll-like receptor-4 (TLR-4) involvement were assessed.					
36736264	6	36	theme	in	1071:1072	arg1	model					1080:1084	an in vitro model	1068:1084	an in vitro model of neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)]	1068:1165	Biological response in terms of antioxidant and anti-inflammatory activity was analyzed on an in vitro model of neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)].					
36736264	11	37	theme	central	1879:1885	arg1	system					1895:1900	central nervous system	1879:1900	central nervous system	1879:1900	Overall, our results show that EU@PLA represents an innovative and effective strategy for the control of inflammatory response in central nervous system.					
36736264	4	38	theme	axonal	711:716	arg1	growth					718:723	axonal growth	711:723	axonal growth in a specific direction	711:747	Aligned fibers were chosen to provide physical cues to guide axonal growth in a specific direction thus restoring the synaptic connection.					
36736264	0	39	theme	poly	20:23	arg1	substrates					50:59	poly(lactic acid) electrospun substrates	20:59	poly(lactic acid) electrospun substrates	20:59	Eumelanin decorated poly(lactic acid) electrospun substrates as a new strategy for spinal cord injury treatment.					
36736264	0	39	theme	poly	20:23	arg1	strategy					70:77	a new strategy	64:77	a new strategy for spinal cord injury treatment	64:110	Eumelanin decorated poly(lactic acid) electrospun substrates as a new strategy for spinal cord injury treatment.					
36736264	10	40	theme	TLR-4	1682:1686	arg1	production					1688:1697	TLR-4 production	1682:1697	TLR-4 production induced by LPS stimulation in presence of EU@PLA	1682:1746	Protein expression analysis revealed a decreased in TLR-4 production induced by LPS stimulation in presence of EU@PLA.					
36736264	5	41	theme	oxygen	957:962	arg1	atmosphere					964:973	oxygen atmosphere	957:973	oxygen atmosphere	957:973	Eumelanin decorated PLA electrospun substrates were produced combining electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere).					
36736264	4	42	theme	Aligned	650:656	arg1	fibers					658:663	Aligned fibers	650:663	Aligned fibers	650:663	Aligned fibers were chosen to provide physical cues to guide axonal growth in a specific direction thus restoring the synaptic connection.					
36736264	10	43	theme	LPS	1710:1712	arg1	stimulation					1714:1724	LPS stimulation	1710:1724	LPS stimulation	1710:1724	Protein expression analysis revealed a decreased in TLR-4 production induced by LPS stimulation in presence of EU@PLA.					
36736264	8	44	theme	nuclear	1367:1373	arg1	kappa-light-chain-enhancer					1382:1407	nuclear factor kappa-light-chain-enhancer	1367:1407	nuclear factor kappa-light-chain-enhancer of activated B cells	1367:1428	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	8	44	theme	nuclear	1367:1373	arg1	species					1358:1364	reactive oxygen species	1342:1364	reactive oxygen species	1342:1364	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	0	45	theme	lactic	25:30	arg1	poly					20:23	poly	20:23	poly(lactic acid) electrospun substrates	20:59	Eumelanin decorated poly(lactic acid) electrospun substrates as a new strategy for spinal cord injury treatment.					
36736264	0	45	theme	lactic	25:30	arg1	acid					32:35	lactic acid	25:35	lactic acid	25:35	Eumelanin decorated poly(lactic acid) electrospun substrates as a new strategy for spinal cord injury treatment.					
36736264	7	46	theme	Cell	1168:1171	arg1	morphology					1173:1182	Cell morphology	1168:1182	Cell morphology	1168:1182	Cell morphology and EU@PLA mechanism of action, in terms of toll-like receptor-4 (TLR-4) involvement were assessed.					
36736264	9	47	theme	action	1550:1555	arg1	dependent					1596:1604	dependent	1596:1604	dependent	1596:1604	Finally, the mechanism of action of EU@PLA in microglia was found to be dependent on the TLR-4 signaling.					
36736264	9	47	theme	action	1550:1555	arg1	mechanism					1537:1545	the mechanism	1533:1545	the mechanism of action of EU@PLA in microglia	1533:1578	Finally, the mechanism of action of EU@PLA in microglia was found to be dependent on the TLR-4 signaling.					
36736264	3	48	theme	poly	486:489	arg1	microfibers					521:531	eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers	469:531	eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers	469:531	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	9	49	theme	EU	1560:1561	arg1	PLA					1563:1565	EU@PLA	1560:1565	EU@PLA in microglia	1560:1578	Finally, the mechanism of action of EU@PLA in microglia was found to be dependent on the TLR-4 signaling.					
36736264	10	50	theme	PLA	1744:1746	arg1	presence					1729:1736	presence	1729:1736	presence of EU@PLA	1729:1746	Protein expression analysis revealed a decreased in TLR-4 production induced by LPS stimulation in presence of EU@PLA.					
36736264	8	51	theme	activated	1412:1420	arg1	cells					1424:1428	activated B cells	1412:1428	activated B cells	1412:1428	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	3	52	from	effect	459:464	arg1	model					545:549	in vitro model	536:549	in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation	536:647	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	3	53	dep	injury	595:600	arg1	modulation					638:647	modulation	638:647	spinal cord injury in terms of inflammatory mediators' modulation	583:647	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	9	54	theme	PLA	1563:1565	arg1	action					1550:1555	action	1550:1555	action of EU@PLA in microglia	1550:1578	Finally, the mechanism of action of EU@PLA in microglia was found to be dependent on the TLR-4 signaling.					
36736264	7	55	theme	involvement	1257:1267	arg1	terms					1219:1223	terms	1219:1223	terms of toll-like receptor-4 (TLR-4) involvement	1219:1267	Cell morphology and EU@PLA mechanism of action, in terms of toll-like receptor-4 (TLR-4) involvement were assessed.					
36736264	11	56	theme	EU	1780:1781	arg1	PLA					1783:1785	EU@PLA	1780:1785	EU@PLA	1780:1785	Overall, our results show that EU@PLA represents an innovative and effective strategy for the control of inflammatory response in central nervous system.					
36736264	3	57	theme	mediators	627:635	arg1	terms					605:609	terms	605:609	terms of inflammatory mediators	605:635	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	8	58	theme	cells	1424:1428	arg1	kappa-light-chain-enhancer					1382:1407	nuclear factor kappa-light-chain-enhancer	1367:1407	nuclear factor kappa-light-chain-enhancer of activated B cells	1367:1428	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	8	58	theme	cells	1424:1428	arg1	species					1358:1364	reactive oxygen species	1342:1364	reactive oxygen species	1342:1364	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	6	59	theme	cells	1119:1123	arg1	model					1080:1084	an in vitro model	1068:1084	an in vitro model of neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)]	1068:1165	Biological response in terms of antioxidant and anti-inflammatory activity was analyzed on an in vitro model of neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)].					
36736264	3	60	theme	lactic	491:496	arg1	poly					486:489	eumelanin-coated poly	469:489	eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers	469:531	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	3	60	theme	lactic	491:496	arg1	acid					498:501	lactic acid	491:501	lactic acid	491:501	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	7	61	theme	receptor-4	1238:1247	arg1	involvement					1257:1267	toll-like receptor-4 (TLR-4) involvement	1228:1267	toll-like receptor-4 (TLR-4) involvement	1228:1267	Cell morphology and EU@PLA mechanism of action, in terms of toll-like receptor-4 (TLR-4) involvement were assessed.					
36736264	3	62	from	injury	595:600	arg1	terms					605:609	terms	605:609	terms of inflammatory mediators	605:635	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	1	63	theme	microglia	235:243	arg1	activation					221:230	an uncontrolled activation	205:230	an uncontrolled activation	205:230	Spinal cord injury (SCI) is characterized by neuroinflammatory processes that are marked by an uncontrolled activation of microglia, which directly damages neurons.					
36736264	2	64	theme	effective	322:330	arg1	tool					332:335	an effective tool	319:335	an effective tool to treat neuroinflammation because they possess immunomodulatory properties	319:411	Natural and synthetic melanins represent an effective tool to treat neuroinflammation because they possess immunomodulatory properties.					
36736264	3	65	theme	EU	505:506	arg1	PLA					508:510	EU@PLA	505:510	EU@PLA	505:510	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	3	65	theme	EU	505:506	arg1	poly					486:489	eumelanin-coated poly	469:489	eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers	469:531	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	11	66	theme	nervous	1887:1893	arg1	system					1895:1900	central nervous system	1879:1900	central nervous system	1879:1900	Overall, our results show that EU@PLA represents an innovative and effective strategy for the control of inflammatory response in central nervous system.					
36736264	2	67	theme	synthetic	290:298	arg1	melanins					300:307	Natural and synthetic melanins	278:307	Natural and synthetic melanins	278:307	Natural and synthetic melanins represent an effective tool to treat neuroinflammation because they possess immunomodulatory properties.					
36736264	6	68	theme	Biological	977:986	arg1	response					988:995	Biological response	977:995	Biological response in terms of antioxidant and anti-inflammatory activity	977:1050	Biological response in terms of antioxidant and anti-inflammatory activity was analyzed on an in vitro model of neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)].					
36736264	9	69	theme	TLR-4	1613:1617	arg1	signaling					1619:1627	the TLR-4 signaling	1609:1627	the TLR-4 signaling	1609:1627	Finally, the mechanism of action of EU@PLA in microglia was found to be dependent on the TLR-4 signaling.					
36736264	2	70	theme	Natural	278:284	arg1	melanins					300:307	Natural and synthetic melanins	278:307	Natural and synthetic melanins	278:307	Natural and synthetic melanins represent an effective tool to treat neuroinflammation because they possess immunomodulatory properties.					
36736264	1	71	theme	neuroinflammatory	158:174	arg1	processes					176:184	neuroinflammatory processes	158:184	neuroinflammatory processes that are marked by an uncontrolled activation of microglia, which directly damages neurons	158:275	Spinal cord injury (SCI) is characterized by neuroinflammatory processes that are marked by an uncontrolled activation of microglia, which directly damages neurons.					
36736264	10	72	theme	@	1743:1743	arg1	PLA					1744:1746	EU@PLA	1741:1746	EU@PLA	1741:1746	Protein expression analysis revealed a decreased in TLR-4 production induced by LPS stimulation in presence of EU@PLA.					
36736264	5	73	theme	electrospun	813:823	arg1	substrates					825:834	Eumelanin decorated PLA electrospun substrates	789:834	Eumelanin decorated PLA electrospun substrates	789:834	Eumelanin decorated PLA electrospun substrates were produced combining electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere).					
36736264	5	74	theme	solid-state	894:904	arg1	polymerization					906:919	solid-state polymerization	894:919	solid-state polymerization	894:919	Eumelanin decorated PLA electrospun substrates were produced combining electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere).					
36736264	3	75	theme	spinal	583:588	arg1	injury					595:600	spinal cord injury	583:600	spinal cord injury in terms of inflammatory mediators' modulation	583:647	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	3	76	theme	aligned	513:519	arg1	microfibers					521:531	eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers	469:531	eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers	469:531	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	6	77	theme	activity	1043:1050	arg1	terms					1000:1004	terms	1000:1004	terms of antioxidant and anti-inflammatory activity	1000:1050	Biological response in terms of antioxidant and anti-inflammatory activity was analyzed on an in vitro model of neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)].					
36736264	10	78	dep	revealed	1658:1665	arg1	a					1667:1667	a	1667:1667	a	1667:1667	Protein expression analysis revealed a decreased in TLR-4 production induced by LPS stimulation in presence of EU@PLA.					
36736264	10	78	dep	revealed	1658:1665	arg1	decreased					1669:1677	decreased	1669:1677	revealed a decreased in TLR-4 production induced by LPS stimulation in presence of EU@PLA	1658:1746	Protein expression analysis revealed a decreased in TLR-4 production induced by LPS stimulation in presence of EU@PLA.					
36736264	3	79	theme	related	572:578	arg1	neuroinflammation					554:570	neuroinflammation	554:570	neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation	554:647	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	5	80	theme	decorated	799:807	arg1	substrates					825:834	Eumelanin decorated PLA electrospun substrates	789:834	Eumelanin decorated PLA electrospun substrates	789:834	Eumelanin decorated PLA electrospun substrates were produced combining electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere).					
36736264	1	81	theme	cord	120:123	arg1	injury					125:130	Spinal cord injury	113:130	Spinal cord injury (SCI)	113:136	Spinal cord injury (SCI) is characterized by neuroinflammatory processes that are marked by an uncontrolled activation of microglia, which directly damages neurons.					
36736264	1	81	theme	cord	120:123	arg1	SCI					133:135	SCI	133:135	SCI	133:135	Spinal cord injury (SCI) is characterized by neuroinflammatory processes that are marked by an uncontrolled activation of microglia, which directly damages neurons.					
36736264	0	82	theme	new	66:68	arg1	substrates					50:59	poly(lactic acid) electrospun substrates	20:59	poly(lactic acid) electrospun substrates	20:59	Eumelanin decorated poly(lactic acid) electrospun substrates as a new strategy for spinal cord injury treatment.					
36736264	0	82	theme	new	66:68	arg1	strategy					70:77	a new strategy	64:77	a new strategy for spinal cord injury treatment	64:110	Eumelanin decorated poly(lactic acid) electrospun substrates as a new strategy for spinal cord injury treatment.					
36736264	5	83	theme	coating	882:888	arg1	processes					921:929	electrospinning, spin coating and solid-state polymerization processes	860:929	electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere)	860:974	Eumelanin decorated PLA electrospun substrates were produced combining electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere).					
36736264	8	84	theme	@	1308:1308	arg1	fibers					1313:1318	EU@PLA fibers	1306:1318	EU@PLA fibers	1306:1318	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	3	85	theme	microfibers	521:531	arg1	effect					459:464	the effect	455:464	the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation	455:647	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	7	86	from	morphology	1173:1182	arg1	terms					1219:1223	terms	1219:1223	terms of toll-like receptor-4 (TLR-4) involvement	1219:1267	Cell morphology and EU@PLA mechanism of action, in terms of toll-like receptor-4 (TLR-4) involvement were assessed.					
36736264	0	87	theme	cord	90:93	arg1	injury					95:100	spinal cord injury	83:100	spinal cord injury treatment	83:110	Eumelanin decorated poly(lactic acid) electrospun substrates as a new strategy for spinal cord injury treatment.					
36736264	4	88	from	growth	718:723	arg1	direction					739:747	a specific direction	728:747	a specific direction	728:747	Aligned fibers were chosen to provide physical cues to guide axonal growth in a specific direction thus restoring the synaptic connection.					
36736264	9	89	from	microglia	1570:1578	arg1	action					1550:1555	action	1550:1555	action of EU@PLA in microglia	1550:1578	Finally, the mechanism of action of EU@PLA in microglia was found to be dependent on the TLR-4 signaling.					
36736264	6	90	from	response	988:995	arg1	terms					1000:1004	terms	1000:1004	terms of antioxidant and anti-inflammatory activity	1000:1050	Biological response in terms of antioxidant and anti-inflammatory activity was analyzed on an in vitro model of neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)].					
36736264	7	91	theme	PLA	1191:1193	arg1	mechanism					1195:1203	EU@PLA mechanism	1188:1203	EU@PLA mechanism	1188:1203	Cell morphology and EU@PLA mechanism of action, in terms of toll-like receptor-4 (TLR-4) involvement were assessed.					
36736264	7	92	theme	EU	1188:1189	arg1	mechanism					1195:1203	EU@PLA mechanism	1188:1203	EU@PLA mechanism	1188:1203	Cell morphology and EU@PLA mechanism of action, in terms of toll-like receptor-4 (TLR-4) involvement were assessed.					
36736264	5	93	theme	oxidative	932:940	arg1	coupling					942:949	oxidative coupling	932:949	oxidative coupling under oxygen atmosphere	932:973	Eumelanin decorated PLA electrospun substrates were produced combining electrospinning, spin coating and solid-state polymerization processes (oxidative coupling under oxygen atmosphere).					
36736264	9	94	from	PLA	1563:1565	arg1	microglia					1570:1578	microglia	1570:1578	microglia	1570:1578	Finally, the mechanism of action of EU@PLA in microglia was found to be dependent on the TLR-4 signaling.					
36736264	6	95	dep	in	1071:1072	arg1	vitro					1074:1078	vitro	1074:1078	vitro	1074:1078	Biological response in terms of antioxidant and anti-inflammatory activity was analyzed on an in vitro model of neuroinflammation [microglial cells stimulated with lipopolysaccharide (LPS)].					
36736264	3	96	theme	main	424:427	arg1	objective					429:437	the main objective	420:437	the main objective	420:437	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	11	97	theme	response	1867:1874	arg1	control					1843:1849	the control	1839:1849	the control of inflammatory response in central nervous system	1839:1900	Overall, our results show that EU@PLA represents an innovative and effective strategy for the control of inflammatory response in central nervous system.					
36736264	8	98	theme	oxygen	1351:1356	arg1	kappa-light-chain-enhancer					1382:1407	nuclear factor kappa-light-chain-enhancer	1367:1407	nuclear factor kappa-light-chain-enhancer of activated B cells	1367:1428	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	8	98	theme	oxygen	1351:1356	arg1	NF-кB					1431:1435	NF-кB	1431:1435	NF-кB	1431:1435	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	8	98	theme	oxygen	1351:1356	arg1	species					1358:1364	reactive oxygen species	1342:1364	reactive oxygen species	1342:1364	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	9	99	from	action	1550:1555	arg1	microglia					1570:1578	microglia	1570:1578	microglia	1570:1578	Finally, the mechanism of action of EU@PLA in microglia was found to be dependent on the TLR-4 signaling.					
36736264	4	100	theme	synaptic	768:775	arg1	connection					777:786	the synaptic connection	764:786	the synaptic connection	764:786	Aligned fibers were chosen to provide physical cues to guide axonal growth in a specific direction thus restoring the synaptic connection.					
36736264	0	101	theme	electrospun	38:48	arg1	substrates					50:59	poly(lactic acid) electrospun substrates	20:59	poly(lactic acid) electrospun substrates	20:59	Eumelanin decorated poly(lactic acid) electrospun substrates as a new strategy for spinal cord injury treatment.					
36736264	0	101	theme	electrospun	38:48	arg1	strategy					70:77	a new strategy	64:77	a new strategy for spinal cord injury treatment	64:110	Eumelanin decorated poly(lactic acid) electrospun substrates as a new strategy for spinal cord injury treatment.					
36736264	8	102	theme	factor	1375:1380	arg1	kappa-light-chain-enhancer					1382:1407	nuclear factor kappa-light-chain-enhancer	1367:1407	nuclear factor kappa-light-chain-enhancer of activated B cells	1367:1428	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	8	102	theme	factor	1375:1380	arg1	species					1358:1364	reactive oxygen species	1342:1364	reactive oxygen species	1342:1364	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	10	103	theme	Protein	1630:1636	arg1	analysis					1649:1656	Protein expression analysis	1630:1656	Protein expression analysis	1630:1656	Protein expression analysis revealed a decreased in TLR-4 production induced by LPS stimulation in presence of EU@PLA.					
36736264	9	104	theme	@	1562:1562	arg1	PLA					1563:1565	EU@PLA	1560:1565	EU@PLA in microglia	1560:1578	Finally, the mechanism of action of EU@PLA in microglia was found to be dependent on the TLR-4 signaling.					
36736264	10	105	theme	EU	1741:1742	arg1	PLA					1744:1746	EU@PLA	1741:1746	EU@PLA	1741:1746	Protein expression analysis revealed a decreased in TLR-4 production induced by LPS stimulation in presence of EU@PLA.					
36736264	3	106	theme	inflammatory	614:625	arg1	mediators					627:635	inflammatory mediators	614:635	inflammatory mediators	614:635	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	3	107	theme	eumelanin-coated	469:484	arg1	poly					486:489	eumelanin-coated poly	469:489	eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers	469:531	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	3	107	theme	eumelanin-coated	469:484	arg1	acid					498:501	lactic acid	491:501	lactic acid	491:501	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	3	107	theme	eumelanin-coated	469:484	arg1	PLA					508:510	EU@PLA	505:510	EU@PLA	505:510	Here, the main objective was to evaluate the effect of eumelanin-coated poly(lactic acid) (EU@PLA) aligned microfibers on in vitro model of neuroinflammation related to spinal cord injury in terms of inflammatory mediators' modulation.					
36736264	11	108	theme	effective	1816:1824	arg1	strategy					1826:1833	an innovative and effective strategy	1798:1833	an innovative and effective strategy for the control of inflammatory response in central nervous system	1798:1900	Overall, our results show that EU@PLA represents an innovative and effective strategy for the control of inflammatory response in central nervous system.					
36736264	8	109	theme	B	1422:1422	arg1	cells					1424:1428	activated B cells	1412:1428	activated B cells	1412:1428	The results show that EU@PLA fibers were able to decrease reactive oxygen species, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) expression >50 % compared to PLA + LPS and interleukin 6 (IL-6) secretion about 20 %.					
36736264	1	110	theme	uncontrolled	208:219	arg1	activation					221:230	an uncontrolled activation	205:230	an uncontrolled activation	205:230	Spinal cord injury (SCI) is characterized by neuroinflammatory processes that are marked by an uncontrolled activation of microglia, which directly damages neurons.					
36142513	9	0	theme	bone	1539:1542	arg1	marrow					1544:1549	bone marrow	1539:1549	bone marrow mesenchymal stem cell (BM-MSC) line (healthy control)	1539:1603	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	6	1	theme	chondrogenic	992:1003	arg1	day					1034:1036	day 35	1034:1039	day 35	1034:1039	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	6	1	theme	chondrogenic	992:1003	arg1	re-differentiation					1014:1031	chondrogenic in vitro re-differentiation	992:1031	chondrogenic in vitro re-differentiation (day 35)	992:1040	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	4	2	theme	total	572:576	arg1	replacement					583:593	total knee replacement	572:593	total knee replacement	572:593	Human osteoarthritic tibial plateaus were obtained from 25 patients undergoing total knee replacement.					
36142513	10	3	from	levels	1746:1751	arg1	BM-MSC					1760:1765	the BM-MSC	1756:1765	the BM-MSC	1756:1765	However, the MPC marker Sox 9 was conserved after in vitro expansion and significantly higher in OA cartilage-derived cells compared to its levels in the BM-MSC.					
36142513	9	4	theme	mesenchymal	1551:1561	arg1	line					1582:1585	bone marrow mesenchymal stem cell (BM-MSC) line	1539:1585	bone marrow mesenchymal stem cell (BM-MSC) line (healthy control)	1539:1603	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	9	4	theme	mesenchymal	1551:1561	arg1	control					1596:1602	healthy control	1588:1602	healthy control	1588:1602	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	14	5	theme	OA	2273:2274	arg1	grades					2276:2281	OA grades	2273:2281	OA grades at the tissue level	2273:2301	MPC marker expression did not differ between OA grades at the tissue level.					
36142513	10	6	theme	marker	1623:1628	arg1	Sox					1630:1632	the MPC marker Sox 9	1615:1634	the MPC marker Sox 9	1615:1634	However, the MPC marker Sox 9 was conserved after in vitro expansion and significantly higher in OA cartilage-derived cells compared to its levels in the BM-MSC.					
36142513	1	7	theme	hyaline-like	253:264	arg1	tissue					276:281	hyaline-like cartilage tissue	253:281	hyaline-like cartilage tissue	253:281	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	6	8	theme	markers-Col	856:866	arg1	A1					870:871	hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1	804:871	A1	870:871	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	9	9	theme	cell	1568:1571	arg1	line					1582:1585	bone marrow mesenchymal stem cell (BM-MSC) line	1539:1585	bone marrow mesenchymal stem cell (BM-MSC) line (healthy control)	1539:1603	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	9	9	theme	cell	1568:1571	arg1	control					1596:1602	healthy control	1588:1602	healthy control	1588:1602	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	6	10	theme	mature	749:754	arg1	markers-Aggrecan					768:783	mature chondrocyte markers-Aggrecan	749:783	mature chondrocyte markers-Aggrecan (Acan)	749:790	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	6	10	theme	mature	749:754	arg1	MPC					708:710	MPC markers-CD105	708:724	MPC markers-CD105	708:724	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	6	10	theme	mature	749:754	arg1	Acan					786:789	Acan	786:789	Acan	786:789	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	7	11	link	OA-derived	1112:1121	arg1	cells					1123:1127	different OA-derived cells	1102:1127	different OA-derived cells	1102:1127	Pellet matrix composition after in vitro chondrogenesis of different OA-derived cells was tested for proteoglycans, collagen II and I by safranin O and immunofluorescence staining.					
36142513	8	12	dep	tissue	1281:1286	arg1	joint					1307:1311	a single OA joint	1295:1311	a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues	1295:1414	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	9	13	theme	BM-MSC	1574:1579	arg1	line					1582:1585	bone marrow mesenchymal stem cell (BM-MSC) line	1539:1585	bone marrow mesenchymal stem cell (BM-MSC) line (healthy control)	1539:1603	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	9	13	theme	BM-MSC	1574:1579	arg1	control					1596:1602	healthy control	1588:1602	healthy control	1588:1602	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	8	14	with	joint	1307:1311	arg1	difference					1333:1342	no significant difference	1318:1342	no significant difference	1318:1342	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	14	15	theme	MPC	2228:2230	arg1	expression					2239:2248	MPC marker expression	2228:2248	MPC marker expression	2228:2248	MPC marker expression did not differ between OA grades at the tissue level.					
36142513	1	16	theme	progenitor	192:201	arg1	MPC					209:211	MPC	209:211	MPC	209:211	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	1	16	theme	progenitor	192:201	arg1	cell					203:206	mesenchymal progenitor cell	180:206	mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue	180:281	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	16	17	theme	clinical	2484:2491	arg1	practice					2493:2500	clinical practice	2484:2500	clinical practice	2484:2500	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	16	18	contain	have	2462:2465	arg1	findings					2449:2456	These findings	2443:2456	These findings	2443:2456	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	16	18	contain	have	2462:2465	arg2	implications					2467:2478	implications	2467:2478	implications for clinical practice	2467:2500	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	12	19	theme	in	1975:1976	arg1	chondrogenesis					1984:1997	the in vitro chondrogenesis	1971:1997	the in vitro chondrogenesis	1971:1997	However, only moderate OA-derived cells after the in vitro chondrogenesis resulted in the formation of hyaline cartilage-like tissue.					
36142513	9	20	theme	healthy	1588:1594	arg1	line					1582:1585	bone marrow mesenchymal stem cell (BM-MSC) line	1539:1585	bone marrow mesenchymal stem cell (BM-MSC) line (healthy control)	1539:1603	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	9	20	theme	healthy	1588:1594	arg1	control					1596:1602	healthy control	1588:1602	healthy control	1588:1602	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	14	21	from	level	2297:2301	arg1	grades					2276:2281	OA grades	2273:2281	OA grades at the tissue level	2273:2301	MPC marker expression did not differ between OA grades at the tissue level.					
36142513	11	22	from	enrichment	1830:1839	arg1	reduction					1869:1877	reduction	1869:1877	reduction of MPC markers for all three analyzed grades	1869:1922	The in vitro expansion of cartilage-derived cells resulted in enrichment while re-differentiation in reduction of MPC markers for all three analyzed grades.					
36142513	1	23	contain	have	227:230	arg1	markers					214:220	mesenchymal progenitor cell (MPC) markers	180:220	mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue	180:281	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	1	23	contain	have	227:230	arg2	capacity					236:243	the capacity to form hyaline-like cartilage tissue	232:281	the capacity to form hyaline-like cartilage tissue	232:281	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	15	24	theme	moderate	2358:2365	arg1	cells					2378:2382	only moderate OA-derived cells	2353:2382	only moderate OA-derived cells	2353:2382	Interestingly after in vitro re-differentiation, only moderate OA-derived cells showed the capacity to form hyaline cartilage-like tissue.					
36142513	16	25	theme	cartilage	2665:2673	arg1	repair					2675:2680	articular cartilage repair	2655:2680	articular cartilage repair	2655:2680	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	12	26	theme	OA-derived	1948:1957	arg1	cells					1959:1963	only moderate OA-derived cells	1934:1963	only moderate OA-derived cells after the in vitro chondrogenesis	1934:1997	However, only moderate OA-derived cells after the in vitro chondrogenesis resulted in the formation of hyaline cartilage-like tissue.					
36142513	10	27	theme	in	1656:1657	arg1	expansion					1665:1673	in vitro expansion	1656:1673	in vitro expansion	1656:1673	However, the MPC marker Sox 9 was conserved after in vitro expansion and significantly higher in OA cartilage-derived cells compared to its levels in the BM-MSC.					
36142513	16	28	theme	repair	2530:2535	arg1	capacity					2537:2544	the intrinsic repair capacity	2516:2544	the intrinsic repair capacity of articular cartilage in OA tissues	2516:2581	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	13	29	theme	markers	2190:2196	arg1	expression					2153:2162	no expression	2150:2162	no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13)	2150:2225	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	16	30	theme	cells	2630:2634	arg1	candidate					2641:2649	a candidate	2639:2649	a candidate for articular cartilage repair	2639:2680	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	16	30	theme	cells	2630:2634	arg1	possibility					2598:2608	the possibility	2594:2608	the possibility of these progenitor cells	2594:2634	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	8	31	theme	Multiple	1224:1231	arg1	markers					1237:1243	Multiple MPC markers	1224:1243	Multiple MPC markers	1224:1243	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	0	32	theme	Grade	78:82	arg1	Differences					84:94	Osteoarthritis Grade Differences	63:94	Osteoarthritis Grade Differences	63:94	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	0	32	theme	Grade	78:82	arg1	Expression					5:14	Gene Expression	0:14	Gene Expression	0:14	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	0	32	theme	Grade	78:82	arg1	Potential					33:41	Chondrogenic Potential	20:41	Chondrogenic Potential	20:41	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	15	33	theme	hyaline	2412:2418	arg1	tissue					2435:2440	hyaline cartilage-like tissue	2412:2440	hyaline cartilage-like tissue	2412:2440	Interestingly after in vitro re-differentiation, only moderate OA-derived cells showed the capacity to form hyaline cartilage-like tissue.					
36142513	11	34	link	cartilage-derived	1794:1810	arg1	cells					1812:1816	cartilage-derived cells	1794:1816	cartilage-derived cells	1794:1816	The in vitro expansion of cartilage-derived cells resulted in enrichment while re-differentiation in reduction of MPC markers for all three analyzed grades.					
36142513	8	35	theme	severe	1398:1403	arg1	tissues					1408:1414	severe OA tissues	1398:1414	severe OA tissues	1398:1414	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	7	36	dep	in	1075:1076	arg1	vitro					1078:1082	vitro	1078:1082	vitro	1078:1082	Pellet matrix composition after in vitro chondrogenesis of different OA-derived cells was tested for proteoglycans, collagen II and I by safranin O and immunofluorescence staining.					
36142513	7	37	theme	OA-derived	1112:1121	arg1	cells					1123:1127	different OA-derived cells	1102:1127	different OA-derived cells	1102:1127	Pellet matrix composition after in vitro chondrogenesis of different OA-derived cells was tested for proteoglycans, collagen II and I by safranin O and immunofluorescence staining.					
36142513	13	38	with	proteoglycans	2131:2143	arg1	expression					2153:2162	no expression	2150:2162	no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13)	2150:2225	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	9	39	theme	CD105	1438:1442	arg1	levels					1428:1433	Expression levels	1417:1433	Expression levels of CD105 and Notch 1	1417:1454	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	11	40	theme	cartilage-derived	1794:1810	arg1	cells					1812:1816	cartilage-derived cells	1794:1816	cartilage-derived cells	1794:1816	The in vitro expansion of cartilage-derived cells resulted in enrichment while re-differentiation in reduction of MPC markers for all three analyzed grades.					
36142513	8	41	theme	OA	1259:1260	arg1	cartilage					1262:1270	OA cartilage	1259:1270	OA cartilage	1259:1270	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	6	42	theme	expression	687:696	arg1	levels					698:703	mRNA expression levels	682:703	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL	682:888	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	3	43	theme	chondrogenetic	417:430	arg1	potential					432:440	chondrogenetic potential	417:440	chondrogenetic potential of cells from mild, moderate and severe OA tissue	417:490	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	9	44	theme	Notch	1448:1452	arg1	levels					1428:1433	Expression levels	1417:1433	Expression levels of CD105 and Notch 1	1417:1454	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	8	45	theme	resident	1272:1279	arg1	tissue					1281:1286	resident tissue	1272:1286	resident tissue	1272:1286	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	0	46	theme	Gene	0:3	arg1	Differences					84:94	Osteoarthritis Grade Differences	63:94	Osteoarthritis Grade Differences	63:94	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	0	46	theme	Gene	0:3	arg1	Expression					5:14	Gene Expression	0:14	Gene Expression	0:14	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	1	47	theme	cartilage	266:274	arg1	tissue					276:281	hyaline-like cartilage tissue	253:281	hyaline-like cartilage tissue	253:281	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	1	48	theme	Recent	97:102	arg1	data					104:107	Recent data	97:107	Recent data	97:107	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	16	49	theme	progenitor	2619:2628	arg1	cells					2630:2634	these progenitor cells	2613:2634	these progenitor cells	2613:2634	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	15	50	dep	in	2324:2325	arg1	vitro					2327:2331	vitro	2327:2331	vitro	2327:2331	Interestingly after in vitro re-differentiation, only moderate OA-derived cells showed the capacity to form hyaline cartilage-like tissue.					
36142513	6	51	theme	expansion	959:967	arg1	weeks					941:945	2 weeks	939:945	2 weeks of in vitro expansion (day 14)	939:976	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	3	52	theme	MPC	391:393	arg1	expression					402:411	MPC marker expression	391:411	MPC marker expression	391:411	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	8	53	theme	single	1297:1302	arg1	joint					1307:1311	a single OA joint	1295:1311	a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues	1295:1414	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	7	54	theme	matrix	1050:1055	arg1	composition					1057:1067	Pellet matrix composition	1043:1067	Pellet matrix composition after in vitro chondrogenesis of different OA-derived cells	1043:1127	Pellet matrix composition after in vitro chondrogenesis of different OA-derived cells was tested for proteoglycans, collagen II and I by safranin O and immunofluorescence staining.					
36142513	12	55	link	OA-derived	1948:1957	arg1	cells					1959:1963	only moderate OA-derived cells	1934:1963	only moderate OA-derived cells after the in vitro chondrogenesis	1934:1997	However, only moderate OA-derived cells after the in vitro chondrogenesis resulted in the formation of hyaline cartilage-like tissue.					
36142513	9	56	theme	cartilage-derived	1483:1499	arg1	cells					1501:1505	OA cartilage-derived cells	1480:1505	OA cartilage-derived cells of different disease grades	1480:1533	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	6	57	theme	in	950:951	arg1	expansion					959:967	in vitro expansion	950:967	in vitro expansion (day 14)	950:976	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	6	57	theme	in	950:951	arg1	day					970:972	day 14	970:975	day 14	970:975	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	4	58	theme	osteoarthritic	499:512	arg1	plateaus					521:528	Human osteoarthritic tibial plateaus	493:528	Human osteoarthritic tibial plateaus	493:528	Human osteoarthritic tibial plateaus were obtained from 25 patients undergoing total knee replacement.					
36142513	10	59	theme	cartilage-derived	1706:1722	arg1	cells					1724:1728	OA cartilage-derived cells	1703:1728	OA cartilage-derived cells	1703:1728	However, the MPC marker Sox 9 was conserved after in vitro expansion and significantly higher in OA cartilage-derived cells compared to its levels in the BM-MSC.					
36142513	0	60	theme	Cartilage	46:54	arg1	Cells					56:60	Cartilage Cells	46:60	Cartilage Cells	46:60	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	11	61	dep	in	1772:1773	arg1	vitro					1775:1779	vitro	1775:1779	vitro	1775:1779	The in vitro expansion of cartilage-derived cells resulted in enrichment while re-differentiation in reduction of MPC markers for all three analyzed grades.					
36142513	7	62	theme	safranin	1180:1187	arg1	O					1189:1189	safranin O	1180:1189	safranin O	1180:1189	Pellet matrix composition after in vitro chondrogenesis of different OA-derived cells was tested for proteoglycans, collagen II and I by safranin O and immunofluorescence staining.					
36142513	12	63	theme	hyaline	2028:2034	arg1	tissue					2051:2056	hyaline cartilage-like tissue	2028:2056	hyaline cartilage-like tissue	2028:2056	However, only moderate OA-derived cells after the in vitro chondrogenesis resulted in the formation of hyaline cartilage-like tissue.					
36142513	14	64	theme	tissue	2290:2295	arg1	level					2297:2301	the tissue level	2286:2301	the tissue level	2286:2301	MPC marker expression did not differ between OA grades at the tissue level.					
36142513	11	65	theme	MPC	1882:1884	arg1	markers					1886:1892	MPC markers	1882:1892	MPC markers for all three analyzed grades	1882:1922	The in vitro expansion of cartilage-derived cells resulted in enrichment while re-differentiation in reduction of MPC markers for all three analyzed grades.					
36142513	9	66	theme	disease	1520:1526	arg1	grades					1528:1533	different disease grades	1510:1533	different disease grades	1510:1533	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	16	67	theme	cartilage	2559:2567	arg1	capacity					2537:2544	the intrinsic repair capacity	2516:2544	the intrinsic repair capacity of articular cartilage in OA tissues	2516:2581	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	12	68	theme	tissue	2051:2056	arg1	formation					2015:2023	the formation	2011:2023	the formation of hyaline cartilage-like tissue	2011:2056	However, only moderate OA-derived cells after the in vitro chondrogenesis resulted in the formation of hyaline cartilage-like tissue.					
36142513	13	69	theme	latter	2063:2068	arg1	samples					2077:2083	The latter tissue samples	2059:2083	The latter tissue samples	2059:2083	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	13	69	theme	latter	2063:2068	arg1	positive					2102:2109	positive	2102:2109	positive	2102:2109	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	8	70	located	found	1250:1254	arg2	markers					1237:1243	Multiple MPC markers	1224:1243	Multiple MPC markers	1224:1243	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	8	70	located	found	1250:1254	arg1	tissue					1281:1286	resident tissue	1272:1286	resident tissue	1272:1286	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	8	70	located	found	1250:1254	arg1	cartilage					1262:1270	OA cartilage	1259:1270	OA cartilage	1259:1270	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	6	71	theme	in	1005:1006	arg1	day					1034:1036	day 35	1034:1039	day 35	1034:1039	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	6	71	theme	in	1005:1006	arg1	re-differentiation					1014:1031	chondrogenic in vitro re-differentiation	992:1031	chondrogenic in vitro re-differentiation (day 35)	992:1040	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	5	72	theme	moderate	632:639	arg1	sample					601:606	Each sample	596:606	Each sample	596:606	Each sample was classified as mild, moderate or severe OA according to OARSI scoring.					
36142513	5	72	theme	moderate	632:639	arg1	OA					651:652	mild, moderate or severe OA	626:652	mild, moderate or severe OA according to OARSI scoring	626:679	Each sample was classified as mild, moderate or severe OA according to OARSI scoring.					
36142513	3	73	from	potential	432:440	arg1	tissue					485:490	mild, moderate and severe OA tissue	456:490	mild, moderate and severe OA tissue	456:490	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	3	74	theme	OA	482:483	arg1	tissue					485:490	mild, moderate and severe OA tissue	456:490	mild, moderate and severe OA tissue	456:490	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	9	75	theme	marrow	1544:1549	arg1	line					1582:1585	bone marrow mesenchymal stem cell (BM-MSC) line	1539:1585	bone marrow mesenchymal stem cell (BM-MSC) line (healthy control)	1539:1603	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	9	75	theme	marrow	1544:1549	arg1	control					1596:1602	healthy control	1588:1602	healthy control	1588:1602	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	1	76	attach	isolated	128:135	arg1	cartilage					162:170	osteoarthritic (OA) cartilage	142:170	osteoarthritic (OA) cartilage	142:170	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	1	76	attach	isolated	128:135	arg2	cells					122:126	cells	122:126	cells isolated from osteoarthritic (OA) cartilage	122:170	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	5	77	theme	severe	644:649	arg1	sample					601:606	Each sample	596:606	Each sample	596:606	Each sample was classified as mild, moderate or severe OA according to OARSI scoring.					
36142513	5	77	theme	severe	644:649	arg1	OA					651:652	mild, moderate or severe OA	626:652	mild, moderate or severe OA according to OARSI scoring	626:679	Each sample was classified as mild, moderate or severe OA according to OARSI scoring.					
36142513	3	78	from	tissue	485:490	arg1	cells					445:449	cells	445:449	cells from mild, moderate and severe OA tissue	445:490	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	3	78	from	tissue	485:490	arg1	expression					402:411	MPC marker expression	391:411	MPC marker expression	391:411	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	3	78	from	tissue	485:490	arg1	potential					432:440	chondrogenetic potential	417:440	chondrogenetic potential of cells from mild, moderate and severe OA tissue	417:490	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	11	79	theme	markers	1886:1892	arg1	reduction					1869:1877	reduction	1869:1877	reduction of MPC markers for all three analyzed grades	1869:1922	The in vitro expansion of cartilage-derived cells resulted in enrichment while re-differentiation in reduction of MPC markers for all three analyzed grades.					
36142513	6	80	theme	I	868:868	arg1	A1					870:871	hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1	804:871	A1	870:871	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	9	81	theme	stem	1563:1566	arg1	line					1582:1585	bone marrow mesenchymal stem cell (BM-MSC) line	1539:1585	bone marrow mesenchymal stem cell (BM-MSC) line (healthy control)	1539:1603	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	9	81	theme	stem	1563:1566	arg1	control					1596:1602	healthy control	1588:1602	healthy control	1588:1602	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	9	82	theme	Expression	1417:1426	arg1	levels					1428:1433	Expression levels	1417:1433	Expression levels of CD105 and Notch 1	1417:1454	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	4	83	theme	knee	578:581	arg1	replacement					583:593	total knee replacement	572:593	total knee replacement	572:593	Human osteoarthritic tibial plateaus were obtained from 25 patients undergoing total knee replacement.					
36142513	16	84	from	capacity	2537:2544	arg1	tissues					2575:2581	OA tissues	2572:2581	OA tissues	2572:2581	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	6	85	theme	chondrocyte	756:766	arg1	markers-Aggrecan					768:783	mature chondrocyte markers-Aggrecan	749:783	mature chondrocyte markers-Aggrecan (Acan)	749:790	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	6	85	theme	chondrocyte	756:766	arg1	MPC					708:710	MPC markers-CD105	708:724	MPC markers-CD105	708:724	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	6	85	theme	chondrocyte	756:766	arg1	Acan					786:789	Acan	786:789	Acan	786:789	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	10	86	theme	MPC	1619:1621	arg1	Sox					1630:1632	the MPC marker Sox 9	1615:1634	the MPC marker Sox 9	1615:1634	However, the MPC marker Sox 9 was conserved after in vitro expansion and significantly higher in OA cartilage-derived cells compared to its levels in the BM-MSC.					
36142513	6	87	theme	osteoarthritis-related	833:854	arg1	A1					870:871	hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1	804:871	A1	870:871	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	13	88	with	collagen	2115:2122	arg1	expression					2153:2162	no expression	2150:2162	no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13)	2150:2225	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	5	89	theme	mild	626:629	arg1	sample					601:606	Each sample	596:606	Each sample	596:606	Each sample was classified as mild, moderate or severe OA according to OARSI scoring.					
36142513	5	89	theme	mild	626:629	arg1	OA					651:652	mild, moderate or severe OA	626:652	mild, moderate or severe OA according to OARSI scoring	626:679	Each sample was classified as mild, moderate or severe OA according to OARSI scoring.					
36142513	14	90	theme	marker	2232:2237	arg1	expression					2239:2248	MPC marker expression	2228:2248	MPC marker expression	2228:2248	MPC marker expression did not differ between OA grades at the tissue level.					
36142513	11	91	theme	analyzed	1908:1915	arg1	grades					1917:1922	all three analyzed grades	1898:1922	all three analyzed grades	1898:1922	The in vitro expansion of cartilage-derived cells resulted in enrichment while re-differentiation in reduction of MPC markers for all three analyzed grades.					
36142513	1	92	theme	mesenchymal	180:190	arg1	MPC					209:211	MPC	209:211	MPC	209:211	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	1	92	theme	mesenchymal	180:190	arg1	cell					203:206	mesenchymal progenitor cell	180:206	mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue	180:281	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	8	93	theme	OA	1405:1406	arg1	tissues					1408:1414	severe OA tissues	1398:1414	severe OA tissues	1398:1414	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	6	94	dep	in	1005:1006	arg1	vitro					1008:1012	vitro	1008:1012	vitro	1008:1012	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	1	95	theme	cell	203:206	arg1	markers					214:220	mesenchymal progenitor cell (MPC) markers	180:220	mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue	180:281	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	6	96	theme	tissue	911:916	arg1	day					925:927	day 0	925:929	day 0	925:929	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	6	96	theme	tissue	911:916	arg1	level					918:922	the tissue level	907:922	the tissue level (day 0)	907:930	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	16	97	theme	OA	2572:2573	arg1	tissues					2575:2581	OA tissues	2572:2581	OA tissues	2572:2581	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	10	98	dep	in	1656:1657	arg1	vitro					1659:1663	vitro	1659:1663	vitro	1659:1663	However, the MPC marker Sox 9 was conserved after in vitro expansion and significantly higher in OA cartilage-derived cells compared to its levels in the BM-MSC.					
36142513	16	99	theme	intrinsic	2520:2528	arg1	capacity					2537:2544	the intrinsic repair capacity	2516:2544	the intrinsic repair capacity of articular cartilage in OA tissues	2516:2581	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	11	100	theme	cells	1812:1816	arg1	expansion					1781:1789	The in vitro expansion	1768:1789	The in vitro expansion of cartilage-derived cells	1768:1816	The in vitro expansion of cartilage-derived cells resulted in enrichment while re-differentiation in reduction of MPC markers for all three analyzed grades.					
36142513	15	101	theme	OA-derived	2367:2376	arg1	cells					2378:2382	only moderate OA-derived cells	2353:2382	only moderate OA-derived cells	2353:2382	Interestingly after in vitro re-differentiation, only moderate OA-derived cells showed the capacity to form hyaline cartilage-like tissue.					
36142513	9	102	link	cartilage-derived	1483:1499	arg1	cells					1501:1505	OA cartilage-derived cells	1480:1505	OA cartilage-derived cells of different disease grades	1480:1533	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	16	103	theme	articular	2655:2663	arg1	repair					2675:2680	articular cartilage repair	2655:2680	articular cartilage repair	2655:2680	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	12	104	theme	moderate	1939:1946	arg1	cells					1959:1963	only moderate OA-derived cells	1934:1963	only moderate OA-derived cells after the in vitro chondrogenesis	1934:1997	However, only moderate OA-derived cells after the in vitro chondrogenesis resulted in the formation of hyaline cartilage-like tissue.					
36142513	15	105	link	OA-derived	2367:2376	arg1	cells					2378:2382	only moderate OA-derived cells	2353:2382	only moderate OA-derived cells	2353:2382	Interestingly after in vitro re-differentiation, only moderate OA-derived cells showed the capacity to form hyaline cartilage-like tissue.					
36142513	5	106	theme	OARSI	667:671	arg1	scoring					673:679	OARSI scoring	667:679	OARSI scoring	667:679	Each sample was classified as mild, moderate or severe OA according to OARSI scoring.					
36142513	10	107	theme	OA	1703:1704	arg1	cells					1724:1728	OA cartilage-derived cells	1703:1728	OA cartilage-derived cells	1703:1728	However, the MPC marker Sox 9 was conserved after in vitro expansion and significantly higher in OA cartilage-derived cells compared to its levels in the BM-MSC.					
36142513	13	108	theme	osteoarthritis-related	2167:2188	arg1	MMP13					2220:2224	MMP13	2220:2224	MMP13	2220:2224	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	13	108	theme	osteoarthritis-related	2167:2188	arg1	collagen					2199:2206	collagen I	2199:2208	collagen I	2199:2208	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	13	108	theme	osteoarthritis-related	2167:2188	arg1	ALPL					2211:2214	ALPL	2211:2214	ALPL	2211:2214	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	13	108	theme	osteoarthritis-related	2167:2188	arg1	markers					2190:2196	osteoarthritis-related markers	2167:2196	osteoarthritis-related markers (collagen I, ALPL and MMP13)	2167:2225	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	0	109	theme	Osteoarthritis	63:76	arg1	Differences					84:94	Osteoarthritis Grade Differences	63:94	Osteoarthritis Grade Differences	63:94	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	0	109	theme	Osteoarthritis	63:76	arg1	Expression					5:14	Gene Expression	0:14	Gene Expression	0:14	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	0	109	theme	Osteoarthritis	63:76	arg1	Potential					33:41	Chondrogenic Potential	20:41	Chondrogenic Potential	20:41	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	1	110	theme	osteoarthritic	142:155	arg1	cartilage					162:170	osteoarthritic (OA) cartilage	142:170	osteoarthritic (OA) cartilage	142:170	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	7	111	theme	cells	1123:1127	arg1	chondrogenesis					1084:1097	in vitro chondrogenesis	1075:1097	in vitro chondrogenesis of different OA-derived cells	1075:1127	Pellet matrix composition after in vitro chondrogenesis of different OA-derived cells was tested for proteoglycans, collagen II and I by safranin O and immunofluorescence staining.					
36142513	15	112	theme	in	2324:2325	arg1	re-differentiation					2333:2350	in vitro re-differentiation	2324:2350	in vitro re-differentiation	2324:2350	Interestingly after in vitro re-differentiation, only moderate OA-derived cells showed the capacity to form hyaline cartilage-like tissue.					
36142513	1	113	theme	OA	158:159	arg1	cartilage					162:170	osteoarthritic (OA) cartilage	142:170	osteoarthritic (OA) cartilage	142:170	Recent data suggest that cells isolated from osteoarthritic (OA) cartilage express mesenchymal progenitor cell (MPC) markers that have the capacity to form hyaline-like cartilage tissue.					
36142513	4	114	theme	Human	493:497	arg1	plateaus					521:528	Human osteoarthritic tibial plateaus	493:528	Human osteoarthritic tibial plateaus	493:528	Human osteoarthritic tibial plateaus were obtained from 25 patients undergoing total knee replacement.					
36142513	8	115	theme	MPC	1233:1235	arg1	markers					1237:1243	Multiple MPC markers	1224:1243	Multiple MPC markers	1224:1243	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	7	116	theme	different	1102:1110	arg1	cells					1123:1127	different OA-derived cells	1102:1127	different OA-derived cells	1102:1127	Pellet matrix composition after in vitro chondrogenesis of different OA-derived cells was tested for proteoglycans, collagen II and I by safranin O and immunofluorescence staining.					
36142513	15	117	theme	cartilage-like	2420:2433	arg1	tissue					2435:2440	hyaline cartilage-like tissue	2412:2440	hyaline cartilage-like tissue	2412:2440	Interestingly after in vitro re-differentiation, only moderate OA-derived cells showed the capacity to form hyaline cartilage-like tissue.					
36142513	13	118	dep	markers	2190:2196	arg1	MMP13					2220:2224	MMP13	2220:2224	MMP13	2220:2224	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	13	118	dep	markers	2190:2196	arg1	collagen					2199:2206	collagen I	2199:2208	collagen I	2199:2208	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	13	118	dep	markers	2190:2196	arg1	ALPL					2211:2214	ALPL	2211:2214	ALPL	2211:2214	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	13	118	dep	markers	2190:2196	arg1	markers					2190:2196	osteoarthritis-related markers	2167:2196	osteoarthritis-related markers (collagen I, ALPL and MMP13)	2167:2225	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	6	119	dep	in	950:951	arg1	vitro					953:957	vitro	953:957	vitro	953:957	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	6	120	theme	mRNA	682:685	arg1	levels					698:703	mRNA expression levels	682:703	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL	682:888	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	10	121	link	cartilage-derived	1706:1722	arg1	cells					1724:1728	OA cartilage-derived cells	1703:1728	OA cartilage-derived cells	1703:1728	However, the MPC marker Sox 9 was conserved after in vitro expansion and significantly higher in OA cartilage-derived cells compared to its levels in the BM-MSC.					
36142513	7	122	theme	in	1075:1076	arg1	chondrogenesis					1084:1097	in vitro chondrogenesis	1075:1097	in vitro chondrogenesis of different OA-derived cells	1075:1127	Pellet matrix composition after in vitro chondrogenesis of different OA-derived cells was tested for proteoglycans, collagen II and I by safranin O and immunofluorescence staining.					
36142513	0	123	dep	Differences	84:94	arg1	Differences					84:94	Osteoarthritis Grade Differences	63:94	Osteoarthritis Grade Differences	63:94	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	0	123	dep	Differences	84:94	arg1	Expression					5:14	Gene Expression	0:14	Gene Expression	0:14	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	0	123	dep	Differences	84:94	arg1	Potential					33:41	Chondrogenic Potential	20:41	Chondrogenic Potential	20:41	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	0	124	theme	Chondrogenic	20:31	arg1	Differences					84:94	Osteoarthritis Grade Differences	63:94	Osteoarthritis Grade Differences	63:94	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	0	124	theme	Chondrogenic	20:31	arg1	Potential					33:41	Chondrogenic Potential	20:41	Chondrogenic Potential	20:41	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	7	125	theme	immunofluorescence	1195:1212	arg1	staining					1214:1221	immunofluorescence staining	1195:1221	immunofluorescence staining	1195:1221	Pellet matrix composition after in vitro chondrogenesis of different OA-derived cells was tested for proteoglycans, collagen II and I by safranin O and immunofluorescence staining.					
36142513	3	126	theme	mild	456:459	arg1	tissue					485:490	mild, moderate and severe OA tissue	456:490	mild, moderate and severe OA tissue	456:490	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	7	127	theme	Pellet	1043:1048	arg1	composition					1057:1067	Pellet matrix composition	1043:1067	Pellet matrix composition after in vitro chondrogenesis of different OA-derived cells	1043:1127	Pellet matrix composition after in vitro chondrogenesis of different OA-derived cells was tested for proteoglycans, collagen II and I by safranin O and immunofluorescence staining.					
36142513	6	128	theme	hypertrophic	804:815	arg1	MPC					708:710	MPC markers-CD105	708:724	MPC markers-CD105	708:724	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	6	128	theme	hypertrophic	804:815	arg1	chondrocyte					817:827	hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1	804:871	chondrocyte	817:827	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	9	129	theme	OA	1480:1481	arg1	cells					1501:1505	OA cartilage-derived cells	1480:1505	OA cartilage-derived cells of different disease grades	1480:1533	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	3	130	theme	marker	395:400	arg1	expression					402:411	MPC marker expression	391:411	MPC marker expression	391:411	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	4	131	theme	tibial	514:519	arg1	plateaus					521:528	Human osteoarthritic tibial plateaus	493:528	Human osteoarthritic tibial plateaus	493:528	Human osteoarthritic tibial plateaus were obtained from 25 patients undergoing total knee replacement.					
36142513	3	132	theme	cells	445:449	arg1	expression					402:411	MPC marker expression	391:411	MPC marker expression	391:411	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	3	132	theme	cells	445:449	arg1	potential					432:440	chondrogenetic potential	417:440	chondrogenetic potential of cells from mild, moderate and severe OA tissue	417:490	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	0	133	theme	Cells	56:60	arg1	Differences					84:94	Osteoarthritis Grade Differences	63:94	Osteoarthritis Grade Differences	63:94	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	0	133	theme	Cells	56:60	arg1	Expression					5:14	Gene Expression	0:14	Gene Expression	0:14	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	0	133	theme	Cells	56:60	arg1	Potential					33:41	Chondrogenic Potential	20:41	Chondrogenic Potential	20:41	Gene Expression and Chondrogenic Potential of Cartilage Cells: Osteoarthritis Grade Differences.					
36142513	12	134	dep	in	1975:1976	arg1	vitro					1978:1982	vitro	1978:1982	vitro	1978:1982	However, only moderate OA-derived cells after the in vitro chondrogenesis resulted in the formation of hyaline cartilage-like tissue.					
36142513	16	135	theme	articular	2549:2557	arg1	cartilage					2559:2567	articular cartilage	2549:2567	articular cartilage	2549:2567	These findings may have implications for clinical practice to understand the intrinsic repair capacity of articular cartilage in OA tissues and raises the possibility of these progenitor cells as a candidate for articular cartilage repair.					
36142513	8	136	theme	OA	1304:1305	arg1	joint					1307:1311	a single OA joint	1295:1311	a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues	1295:1414	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	3	137	theme	severe	475:480	arg1	tissue					485:490	mild, moderate and severe OA tissue	456:490	mild, moderate and severe OA tissue	456:490	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	12	138	theme	cartilage-like	2036:2049	arg1	tissue					2051:2056	hyaline cartilage-like tissue	2028:2056	hyaline cartilage-like tissue	2028:2056	However, only moderate OA-derived cells after the in vitro chondrogenesis resulted in the formation of hyaline cartilage-like tissue.					
36142513	6	139	theme	Col	793:795	arg1	MPC					708:710	MPC markers-CD105	708:724	MPC markers-CD105	708:724	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	6	139	theme	Col	793:795	arg1	A1					800:801	Col II A1	793:801	Col II A1	793:801	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	9	140	theme	different	1510:1518	arg1	grades					1528:1533	different disease grades	1510:1533	different disease grades	1510:1533	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	3	141	from	expression	402:411	arg1	tissue					485:490	mild, moderate and severe OA tissue	456:490	mild, moderate and severe OA tissue	456:490	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	3	142	theme	moderate	462:469	arg1	tissue					485:490	mild, moderate and severe OA tissue	456:490	mild, moderate and severe OA tissue	456:490	Therefore, we analyzed MPC marker expression and chondrogenetic potential of cells from mild, moderate and severe OA tissue.					
36142513	9	143	theme	grades	1528:1533	arg1	cells					1501:1505	OA cartilage-derived cells	1480:1505	OA cartilage-derived cells of different disease grades	1480:1533	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	9	143	theme	grades	1528:1533	arg1	line					1582:1585	bone marrow mesenchymal stem cell (BM-MSC) line	1539:1585	bone marrow mesenchymal stem cell (BM-MSC) line (healthy control)	1539:1603	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	9	143	theme	grades	1528:1533	arg1	control					1596:1602	healthy control	1588:1602	healthy control	1588:1602	Expression levels of CD105 and Notch 1 were comparable between OA cartilage-derived cells of different disease grades and bone marrow mesenchymal stem cell (BM-MSC) line (healthy control).					
36142513	11	144	theme	in	1772:1773	arg1	expansion					1781:1789	The in vitro expansion	1768:1789	The in vitro expansion of cartilage-derived cells	1768:1816	The in vitro expansion of cartilage-derived cells resulted in enrichment while re-differentiation in reduction of MPC markers for all three analyzed grades.					
36142513	8	145	theme	significant	1321:1331	arg1	difference					1333:1342	no significant difference	1318:1342	no significant difference	1318:1342	Multiple MPC markers were found in OA cartilage resident tissue within a single OA joint with no significant difference between grades except for Notch1, which was higher in severe OA tissues.					
36142513	6	146	theme	MPC	708:710	arg1	levels					698:703	mRNA expression levels	682:703	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL	682:888	mRNA expression levels of MPC markers-CD105, CD166, Notch 1, Sox9; mature chondrocyte markers-Aggrecan (Acan), Col II A1, hypertrophic chondrocyte and osteoarthritis-related markers-Col I A1, MMP-13 and ALPL were measured at the tissue level (day 0), after 2 weeks of in vitro expansion (day 14) and following chondrogenic in vitro re-differentiation (day 35).					
36142513	2	147	theme	OA	345:346	arg1	severity					333:340	the severity	329:340	the severity of OA	329:346	Whether or not these cells are influenced by the severity of OA remains unexplored.					
36142513	13	148	theme	tissue	2070:2075	arg1	samples					2077:2083	The latter tissue samples	2059:2083	The latter tissue samples	2059:2083	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36142513	13	148	theme	tissue	2070:2075	arg1	positive					2102:2109	positive	2102:2109	positive	2102:2109	The latter tissue samples were also highly positive for collagen II and proteoglycans with no expression of osteoarthritis-related markers (collagen I, ALPL and MMP13).					
36241299	0	0	theme	bone	101:104	arg1	repair					106:111	maxillofacial bone repair	87:111	maxillofacial bone repair	87:111	nHA-loaded gelatin/alginate hydrogel with combined physical and bioactive features for maxillofacial bone repair.					
36241299	5	1	theme	interactive	797:807	arg1	surface					776:782	The highly ordered, homogeneous and size-confined porous surface	719:782	The highly ordered, homogeneous and size-confined porous surface	719:782	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	5	1	theme	interactive	797:807	arg1	platform					820:827	an interactive osteogenic platform	794:827	an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs)	794:922	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	5	2	link	derived	897:903	arg1	BMSCs					917:921	BMSCs	917:921	BMSCs	917:921	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	5	2	link	derived	897:903	arg1	cells					910:914	bone marrow derived stem cells	885:914	bone marrow derived stem cells (BMSCs)	885:922	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	1	3	theme	clinical	174:181	arg1	defects					148:154	Critical-sized maxillofacial bone defects	114:154	Critical-sized maxillofacial bone defects	114:154	Critical-sized maxillofacial bone defects have been a tough clinical challenge considering their requirements for functional and structural repair.					
36241299	1	3	theme	clinical	174:181	arg1	challenge					183:191	a tough clinical challenge	166:191	a tough clinical challenge considering their requirements for functional and structural repair	166:259	Critical-sized maxillofacial bone defects have been a tough clinical challenge considering their requirements for functional and structural repair.					
36241299	0	4	theme	maxillofacial	87:99	arg1	repair					106:111	maxillofacial bone repair	87:111	maxillofacial bone repair	87:111	nHA-loaded gelatin/alginate hydrogel with combined physical and bioactive features for maxillofacial bone repair.					
36241299	2	5	theme	20 mg/mL	369:376	arg1	ADA					399:401	ADA	399:401	ADA	399:401	In this study, an injectable in-situ forming double cross-linked hydrogel was prepared from gelatin (Gel), 20 mg/mL alginate dialdehyde (ADA), 4.5 mg/mL Ca2+ and borax.					
36241299	2	5	theme	20 mg/mL	369:376	arg1	dialdehyde					387:396	20 mg/mL alginate dialdehyde	369:396	20 mg/mL alginate dialdehyde (ADA)	369:402	In this study, an injectable in-situ forming double cross-linked hydrogel was prepared from gelatin (Gel), 20 mg/mL alginate dialdehyde (ADA), 4.5 mg/mL Ca2+ and borax.					
36241299	7	6	theme	promising	1177:1185	arg1	hydrogel					1134:1141	the composite hydrogel	1120:1141	the composite hydrogel constructed here	1120:1158	Collectively, the composite hydrogel constructed here might serve as a promising alternative in repair process of complex maxillofacial bone defects.					
36241299	7	6	theme	promising	1177:1185	arg1	alternative					1187:1197	a promising alternative	1175:1197	a promising alternative in repair process of complex maxillofacial bone defects	1175:1253	Collectively, the composite hydrogel constructed here might serve as a promising alternative in repair process of complex maxillofacial bone defects.					
36241299	6	7	theme	defect	1098:1103	arg1	repair					1063:1068	the repair	1059:1068	the repair of critical-size skull bone defect	1059:1103	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	5	8	theme	porous	769:774	arg1	surface					776:782	The highly ordered, homogeneous and size-confined porous surface	719:782	The highly ordered, homogeneous and size-confined porous surface	719:782	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	5	8	theme	porous	769:774	arg1	platform					820:827	an interactive osteogenic platform	794:827	an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs)	794:922	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	2	9	theme	cross-linked	314:325	arg1	hydrogel					327:334	double cross-linked hydrogel	307:334	double cross-linked hydrogel	307:334	In this study, an injectable in-situ forming double cross-linked hydrogel was prepared from gelatin (Gel), 20 mg/mL alginate dialdehyde (ADA), 4.5 mg/mL Ca2+ and borax.					
36241299	3	10	theme	facial	556:561	arg1	structures					563:572	facial structures	556:572	facial structures	556:572	Improved properties of composite hydrogel might well fit and cover irregular geometric shape of facial bone defects, support facial structures and conduct masticatory force.					
36241299	5	11	dep	ordered	730:736	arg1	size-confined					755:767	size-confined	755:767	size-confined	755:767	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	5	11	dep	ordered	730:736	arg1	homogeneous					739:749	homogeneous	739:749	homogeneous	739:749	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	3	12	theme	irregular	498:506	arg1	shape					518:522	irregular geometric shape	498:522	irregular geometric shape of facial bone defects	498:545	Improved properties of composite hydrogel might well fit and cover irregular geometric shape of facial bone defects, support facial structures and conduct masticatory force.					
36241299	2	13	theme	double	307:312	arg1	hydrogel					327:334	double cross-linked hydrogel	307:334	double cross-linked hydrogel	307:334	In this study, an injectable in-situ forming double cross-linked hydrogel was prepared from gelatin (Gel), 20 mg/mL alginate dialdehyde (ADA), 4.5 mg/mL Ca2+ and borax.					
36241299	2	14	theme	4.5 mg/mL	405:413	arg1	Ca2+					415:418	4.5 mg/mL Ca2+	405:418	4.5 mg/mL Ca2+	405:418	In this study, an injectable in-situ forming double cross-linked hydrogel was prepared from gelatin (Gel), 20 mg/mL alginate dialdehyde (ADA), 4.5 mg/mL Ca2+ and borax.					
36241299	6	15	theme	Effective	925:933	arg1	crosstalk					951:959	Effective macrophage-BMSC crosstalk	925:959	Effective macrophage-BMSC crosstalk	925:959	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	3	16	theme	composite	454:462	arg1	hydrogel					464:471	composite hydrogel	454:471	composite hydrogel	454:471	Improved properties of composite hydrogel might well fit and cover irregular geometric shape of facial bone defects, support facial structures and conduct masticatory force.					
36241299	7	17	theme	bone	1242:1245	arg1	defects					1247:1253	complex maxillofacial bone defects	1220:1253	complex maxillofacial bone defects	1220:1253	Collectively, the composite hydrogel constructed here might serve as a promising alternative in repair process of complex maxillofacial bone defects.					
36241299	5	18	theme	osteogenic	809:818	arg1	surface					776:782	The highly ordered, homogeneous and size-confined porous surface	719:782	The highly ordered, homogeneous and size-confined porous surface	719:782	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	5	18	theme	osteogenic	809:818	arg1	platform					820:827	an interactive osteogenic platform	794:827	an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs)	794:922	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	6	19	theme	macrophage-BMSC	935:949	arg1	crosstalk					951:959	Effective macrophage-BMSC crosstalk	925:959	Effective macrophage-BMSC crosstalk	925:959	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	3	20	theme	Improved	431:438	arg1	properties					440:449	Improved properties	431:449	Improved properties of composite hydrogel	431:471	Improved properties of composite hydrogel might well fit and cover irregular geometric shape of facial bone defects, support facial structures and conduct masticatory force.					
36241299	7	21	theme	defects	1247:1253	arg1	process					1209:1215	repair process	1202:1215	repair process of complex maxillofacial bone defects	1202:1253	Collectively, the composite hydrogel constructed here might serve as a promising alternative in repair process of complex maxillofacial bone defects.					
36241299	6	22	theme	@	1046:1046	arg1	Gel/ADA					1047:1053	nHA@Gel/ADA	1043:1053	nHA@Gel/ADA	1043:1053	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	6	22	theme	@	1046:1046	arg1	hydrogel					1033:1040	nHA-loaded gelatin/alginate hydrogel	1005:1040	nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA)	1005:1054	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	3	23	theme	masticatory	586:596	arg1	force					598:602	masticatory force	586:602	masticatory force	586:602	Improved properties of composite hydrogel might well fit and cover irregular geometric shape of facial bone defects, support facial structures and conduct masticatory force.					
36241299	7	24	theme	composite	1124:1132	arg1	alternative					1187:1197	a promising alternative	1175:1197	a promising alternative in repair process of complex maxillofacial bone defects	1175:1253	Collectively, the composite hydrogel constructed here might serve as a promising alternative in repair process of complex maxillofacial bone defects.					
36241299	7	24	theme	composite	1124:1132	arg1	hydrogel					1134:1141	the composite hydrogel	1120:1141	the composite hydrogel constructed here	1120:1158	Collectively, the composite hydrogel constructed here might serve as a promising alternative in repair process of complex maxillofacial bone defects.					
36241299	5	25	theme	derived	897:903	arg1	BMSCs					917:921	BMSCs	917:921	BMSCs	917:921	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	5	25	theme	derived	897:903	arg1	cells					910:914	bone marrow derived stem cells	885:914	bone marrow derived stem cells (BMSCs)	885:922	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	6	26	theme	nHA	1043:1045	arg1	Gel/ADA					1047:1053	nHA@Gel/ADA	1043:1053	nHA@Gel/ADA	1043:1053	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	6	26	theme	nHA	1043:1045	arg1	hydrogel					1033:1040	nHA-loaded gelatin/alginate hydrogel	1005:1040	nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA)	1005:1054	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	5	27	theme	ordered	730:736	arg1	surface					776:782	The highly ordered, homogeneous and size-confined porous surface	719:782	The highly ordered, homogeneous and size-confined porous surface	719:782	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	5	27	theme	ordered	730:736	arg1	platform					820:827	an interactive osteogenic platform	794:827	an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs)	794:922	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	1	28	theme	Critical-sized	114:127	arg1	defects					148:154	Critical-sized maxillofacial bone defects	114:154	Critical-sized maxillofacial bone defects	114:154	Critical-sized maxillofacial bone defects have been a tough clinical challenge considering their requirements for functional and structural repair.					
36241299	1	28	theme	Critical-sized	114:127	arg1	challenge					183:191	a tough clinical challenge	166:191	a tough clinical challenge considering their requirements for functional and structural repair	166:259	Critical-sized maxillofacial bone defects have been a tough clinical challenge considering their requirements for functional and structural repair.					
36241299	1	29	theme	functional	228:237	arg1	repair					254:259	functional and structural repair	228:259	functional and structural repair	228:259	Critical-sized maxillofacial bone defects have been a tough clinical challenge considering their requirements for functional and structural repair.					
36241299	7	30	theme	maxillofacial	1228:1240	arg1	defects					1247:1253	complex maxillofacial bone defects	1220:1253	complex maxillofacial bone defects	1220:1253	Collectively, the composite hydrogel constructed here might serve as a promising alternative in repair process of complex maxillofacial bone defects.					
36241299	3	31	theme	defects	539:545	arg1	shape					518:522	irregular geometric shape	498:522	irregular geometric shape of facial bone defects	498:545	Improved properties of composite hydrogel might well fit and cover irregular geometric shape of facial bone defects, support facial structures and conduct masticatory force.					
36241299	1	32	theme	maxillofacial	129:141	arg1	defects					148:154	Critical-sized maxillofacial bone defects	114:154	Critical-sized maxillofacial bone defects	114:154	Critical-sized maxillofacial bone defects have been a tough clinical challenge considering their requirements for functional and structural repair.					
36241299	1	32	theme	maxillofacial	129:141	arg1	challenge					183:191	a tough clinical challenge	166:191	a tough clinical challenge considering their requirements for functional and structural repair	166:259	Critical-sized maxillofacial bone defects have been a tough clinical challenge considering their requirements for functional and structural repair.					
36241299	3	33	theme	facial	527:532	arg1	defects					539:545	facial bone defects	527:545	facial bone defects	527:545	Improved properties of composite hydrogel might well fit and cover irregular geometric shape of facial bone defects, support facial structures and conduct masticatory force.					
36241299	0	34	theme	physical	51:58	arg1	features					74:81	combined physical and bioactive features	42:81	combined physical and bioactive features	42:81	nHA-loaded gelatin/alginate hydrogel with combined physical and bioactive features for maxillofacial bone repair.					
36241299	2	35	link	cross-linked	314:325	arg1	hydrogel					327:334	double cross-linked hydrogel	307:334	double cross-linked hydrogel	307:334	In this study, an injectable in-situ forming double cross-linked hydrogel was prepared from gelatin (Gel), 20 mg/mL alginate dialdehyde (ADA), 4.5 mg/mL Ca2+ and borax.					
36241299	3	36	theme	hydrogel	464:471	arg1	properties					440:449	Improved properties	431:449	Improved properties of composite hydrogel	431:471	Improved properties of composite hydrogel might well fit and cover irregular geometric shape of facial bone defects, support facial structures and conduct masticatory force.					
36241299	6	37	theme	hydrogel	1033:1040	arg1	efficiency					991:1000	the remarkable efficiency	976:1000	the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect	976:1103	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	1	38	theme	structural	243:252	arg1	repair					254:259	functional and structural repair	228:259	functional and structural repair	228:259	Critical-sized maxillofacial bone defects have been a tough clinical challenge considering their requirements for functional and structural repair.					
36241299	0	39	theme	combined	42:49	arg1	features					74:81	combined physical and bioactive features	42:81	combined physical and bioactive features	42:81	nHA-loaded gelatin/alginate hydrogel with combined physical and bioactive features for maxillofacial bone repair.					
36241299	6	40	theme	gelatin/alginate	1016:1031	arg1	Gel/ADA					1047:1053	nHA@Gel/ADA	1043:1053	nHA@Gel/ADA	1043:1053	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	6	40	theme	gelatin/alginate	1016:1031	arg1	hydrogel					1033:1040	nHA-loaded gelatin/alginate hydrogel	1005:1040	nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA)	1005:1054	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	7	41	theme	complex	1220:1226	arg1	defects					1247:1253	complex maxillofacial bone defects	1220:1253	complex maxillofacial bone defects	1220:1253	Collectively, the composite hydrogel constructed here might serve as a promising alternative in repair process of complex maxillofacial bone defects.					
36241299	5	42	theme	stem	905:908	arg1	BMSCs					917:921	BMSCs	917:921	BMSCs	917:921	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	5	42	theme	stem	905:908	arg1	cells					910:914	bone marrow derived stem cells	885:914	bone marrow derived stem cells (BMSCs)	885:922	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	0	43	theme	bioactive	64:72	arg1	features					74:81	combined physical and bioactive features	42:81	combined physical and bioactive features	42:81	nHA-loaded gelatin/alginate hydrogel with combined physical and bioactive features for maxillofacial bone repair.					
36241299	6	44	theme	nHA-loaded	1005:1014	arg1	Gel/ADA					1047:1053	nHA@Gel/ADA	1043:1053	nHA@Gel/ADA	1043:1053	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	6	44	theme	nHA-loaded	1005:1014	arg1	hydrogel					1033:1040	nHA-loaded gelatin/alginate hydrogel	1005:1040	nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA)	1005:1054	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	0	45	with	hydrogel	28:35	arg1	features					74:81	combined physical and bioactive features	42:81	combined physical and bioactive features	42:81	nHA-loaded gelatin/alginate hydrogel with combined physical and bioactive features for maxillofacial bone repair.					
36241299	1	46	theme	bone	143:146	arg1	defects					148:154	Critical-sized maxillofacial bone defects	114:154	Critical-sized maxillofacial bone defects	114:154	Critical-sized maxillofacial bone defects have been a tough clinical challenge considering their requirements for functional and structural repair.					
36241299	1	46	theme	bone	143:146	arg1	challenge					183:191	a tough clinical challenge	166:191	a tough clinical challenge considering their requirements for functional and structural repair	166:259	Critical-sized maxillofacial bone defects have been a tough clinical challenge considering their requirements for functional and structural repair.					
36241299	7	47	theme	repair	1202:1207	arg1	process					1209:1215	repair process	1202:1215	repair process of complex maxillofacial bone defects	1202:1253	Collectively, the composite hydrogel constructed here might serve as a promising alternative in repair process of complex maxillofacial bone defects.					
36241299	6	48	from	efficiency	991:1000	arg1	repair					1063:1068	the repair	1059:1068	the repair of critical-size skull bone defect	1059:1103	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	3	49	theme	geometric	508:516	arg1	shape					518:522	irregular geometric shape	498:522	irregular geometric shape of facial bone defects	498:545	Improved properties of composite hydrogel might well fit and cover irregular geometric shape of facial bone defects, support facial structures and conduct masticatory force.					
36241299	7	50	from	alternative	1187:1197	arg1	process					1209:1215	repair process	1202:1215	repair process of complex maxillofacial bone defects	1202:1253	Collectively, the composite hydrogel constructed here might serve as a promising alternative in repair process of complex maxillofacial bone defects.					
36241299	4	51	with	decoration	670:679	arg1	hydroxyapatite					697:710	nano-sized hydroxyapatite	686:710	nano-sized hydroxyapatite (nHA)	686:716	We innovatively constructed a bioactive poly-porous structure by decoration with nano-sized hydroxyapatite (nHA).					
36241299	4	51	with	decoration	670:679	arg1	nHA					713:715	nHA	713:715	nHA	713:715	We innovatively constructed a bioactive poly-porous structure by decoration with nano-sized hydroxyapatite (nHA).					
36241299	5	52	dep	derived	897:903	arg1	marrow					890:895	bone marrow	885:895	bone marrow	885:895	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	6	53	theme	skull	1087:1091	arg1	defect					1098:1103	critical-size skull bone defect	1073:1103	critical-size skull bone defect	1073:1103	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	1	54	theme	tough	168:172	arg1	defects					148:154	Critical-sized maxillofacial bone defects	114:154	Critical-sized maxillofacial bone defects	114:154	Critical-sized maxillofacial bone defects have been a tough clinical challenge considering their requirements for functional and structural repair.					
36241299	1	54	theme	tough	168:172	arg1	challenge					183:191	a tough clinical challenge	166:191	a tough clinical challenge considering their requirements for functional and structural repair	166:259	Critical-sized maxillofacial bone defects have been a tough clinical challenge considering their requirements for functional and structural repair.					
36241299	6	55	theme	critical-size	1073:1085	arg1	defect					1098:1103	critical-size skull bone defect	1073:1103	critical-size skull bone defect	1073:1103	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	6	56	theme	bone	1093:1096	arg1	defect					1098:1103	critical-size skull bone defect	1073:1103	critical-size skull bone defect	1073:1103	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	5	57	theme	bone	885:888	arg1	marrow					890:895	bone marrow	885:895	bone marrow	885:895	The highly ordered, homogeneous and size-confined porous surface served as an interactive osteogenic platform for communication and interplay between macrophages and bone marrow derived stem cells (BMSCs).					
36241299	2	58	theme	alginate	378:385	arg1	ADA					399:401	ADA	399:401	ADA	399:401	In this study, an injectable in-situ forming double cross-linked hydrogel was prepared from gelatin (Gel), 20 mg/mL alginate dialdehyde (ADA), 4.5 mg/mL Ca2+ and borax.					
36241299	2	58	theme	alginate	378:385	arg1	dialdehyde					387:396	20 mg/mL alginate dialdehyde	369:396	20 mg/mL alginate dialdehyde (ADA)	369:402	In this study, an injectable in-situ forming double cross-linked hydrogel was prepared from gelatin (Gel), 20 mg/mL alginate dialdehyde (ADA), 4.5 mg/mL Ca2+ and borax.					
36241299	4	59	theme	nano-sized	686:695	arg1	hydroxyapatite					697:710	nano-sized hydroxyapatite	686:710	nano-sized hydroxyapatite (nHA)	686:716	We innovatively constructed a bioactive poly-porous structure by decoration with nano-sized hydroxyapatite (nHA).					
36241299	4	59	theme	nano-sized	686:695	arg1	nHA					713:715	nHA	713:715	nHA	713:715	We innovatively constructed a bioactive poly-porous structure by decoration with nano-sized hydroxyapatite (nHA).					
36241299	4	60	theme	poly-porous	645:655	arg1	structure					657:665	a bioactive poly-porous structure	633:665	a bioactive poly-porous structure	633:665	We innovatively constructed a bioactive poly-porous structure by decoration with nano-sized hydroxyapatite (nHA).					
36241299	6	61	theme	remarkable	980:989	arg1	efficiency					991:1000	the remarkable efficiency	976:1000	the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect	976:1103	Effective macrophage-BMSC crosstalk well explained the remarkable efficiency of nHA-loaded gelatin/alginate hydrogel (nHA@Gel/ADA) in the repair of critical-size skull bone defect.					
36241299	3	62	theme	bone	534:537	arg1	defects					539:545	facial bone defects	527:545	facial bone defects	527:545	Improved properties of composite hydrogel might well fit and cover irregular geometric shape of facial bone defects, support facial structures and conduct masticatory force.					
36241299	4	63	theme	bioactive	635:643	arg1	structure					657:665	a bioactive poly-porous structure	633:665	a bioactive poly-porous structure	633:665	We innovatively constructed a bioactive poly-porous structure by decoration with nano-sized hydroxyapatite (nHA).					
36248846	14	0	theme	phase	2520:2524	arg1	reactants					2526:2534	diverse classical immune and acute phase reactants	2485:2534	diverse classical immune and acute phase reactants	2485:2534	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	7	1	theme	several	1220:1226	arg1	markers					1247:1253	several acute inflammatory markers	1220:1253	several acute inflammatory markers at the protein level	1220:1274	Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level.					
36248846	14	2	theme	Biological	2414:2423	arg1	analysis					2433:2440	Biological pathway analysis	2414:2440	Biological pathway analysis	2414:2440	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	9	3	theme	plasma	1573:1578	arg1	proteome					1580:1587	the plasma proteome	1569:1587	the plasma proteome	1569:1587	First, the bioinformatic analysis revealed the composition of the plasma proteome.					
36248846	11	4	theme	proteins	1881:1888	arg1	got					1890:1892	the control group 62 proteins got	1860:1892	the control group 62 proteins got	1860:1892	Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant (p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated.					
36248846	14	5	theme	classical	2493:2501	arg1	reactants					2526:2534	diverse classical immune and acute phase reactants	2485:2534	diverse classical immune and acute phase reactants	2485:2534	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	10	6	theme	treated	1660:1666	arg1	groups					1680:1685	the treated and control groups	1656:1685	the treated and control groups	1656:1685	Interestingly, 72 commonly expressed proteins were recorded among the treated and control groups, but, surprisingly, 2830 novel proteins were differentially expressed exclusively in the λ-CGN-induced group.					
36248846	4	7	theme	inflammation	689:700	arg1	milieu					664:669	the inflammatory milieu	647:669	the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio)	647:823	Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio).					
36248846	0	8	theme	Plasma	0:5	arg1	responses					16:24	Plasma proteome responses	0:24	Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation	0:82	Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts.					
36248846	13	9	theme	3D	2346:2347	arg1	reconstructions					2349:2363	overlapping 3D reconstructions	2334:2363	overlapping 3D reconstructions between ZF and humans	2334:2385	Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation.					
36248846	12	10	theme	induced	2140:2146	arg1	group					2148:2152	the λ-CGN induced group	2130:2152	the λ-CGN induced group	2130:2152	Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group.					
36248846	5	11	theme	polymorphonuclear	840:856	arg1	neutrophils					870:880	neutrophils	870:880	neutrophils	870:880	We found that polymorphonuclear leukocytes (neutrophils) and lymphocytes infiltrating the ZF peritoneal cavity had short-term persistence.					
36248846	5	11	theme	polymorphonuclear	840:856	arg1	leukocytes					858:867	polymorphonuclear leukocytes	840:867	polymorphonuclear leukocytes (neutrophils)	840:881	We found that polymorphonuclear leukocytes (neutrophils) and lymphocytes infiltrating the ZF peritoneal cavity had short-term persistence.					
36248846	7	12	from	level	1270:1274	arg1	markers					1247:1253	several acute inflammatory markers	1220:1253	several acute inflammatory markers at the protein level	1220:1274	Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level.					
36248846	7	12	from	level	1270:1274	arg1	overexpression					1202:1215	the overexpression	1198:1215	the overexpression of several acute inflammatory markers at the protein level	1198:1274	Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level.					
36248846	4	13	theme	inflammatory	651:662	arg1	milieu					664:669	the inflammatory milieu	647:669	the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio)	647:823	Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio).					
36248846	5	14	theme	peritoneal	919:928	arg1	cavity					930:935	the ZF peritoneal cavity	912:935	the ZF peritoneal cavity	912:935	We found that polymorphonuclear leukocytes (neutrophils) and lymphocytes infiltrating the ZF peritoneal cavity had short-term persistence.					
36248846	2	15	theme	cell	328:331	arg1	wall					333:336	the cell wall	324:336	the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation	324:520	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	11	16	theme	p	1909:1909	arg1	<					1911:1911	p < 0.05	1909:1916	p < 0.05	1909:1916	Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant (p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated.					
36248846	0	17	theme	human	147:151	arg1	counterparts					153:164	their human counterparts	141:164	their human counterparts	141:164	Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts.					
36248846	12	18	from	clusters	2098:2105	arg1	plasma					2120:2125	the blood plasma	2110:2125	the blood plasma of the λ-CGN induced group	2110:2152	Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group.					
36248846	11	19	from	upregulation	1919:1930	arg1	group					1953:1957	the λ-CGN-treated group	1935:1957	the λ-CGN-treated group	1935:1957	Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant (p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated.					
36248846	12	20	theme	hub	2086:2088	arg1	clusters					2098:2105	hub protein clusters	2086:2105	hub protein clusters in the blood plasma of the λ-CGN induced group	2086:2152	Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group.					
36248846	11	21	theme	significant	1896:1906	arg1	upregulation					1919:1930	a significant (p < 0.05) upregulation	1894:1930	a significant (p < 0.05) upregulation in the λ-CGN-treated group	1894:1957	Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant (p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated.					
36248846	8	22	theme	high-performance	1298:1313	arg1	chromatography					1322:1335	reversed-phase high-performance liquid chromatography	1283:1335	reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1283:1417	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	13	23	theme	structural	2289:2298	arg1	analysis					2311:2318	a bioinformatic structural similarity analysis	2273:2318	a bioinformatic structural similarity analysis	2273:2318	Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation.					
36248846	8	24	theme	3.5	1495:1497	arg1	%					1498:1498	%	1498:1498	%	1498:1498	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	12	25	theme	protein-protein	2041:2055	arg1	networks					2069:2076	the major protein-protein interaction networks	2031:2076	the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group	2031:2152	Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group.					
36248846	15	26	theme	inflammatory	2735:2746	arg1	diseases					2748:2755	human intestinal inflammatory diseases	2718:2755	human intestinal inflammatory diseases	2718:2755	Together, this information can be used for testing and finding novel pharmacological targets to treat human intestinal inflammatory diseases.					
36248846	11	27	theme	remaining	1970:1978	arg1	proteins					1984:1991	the remaining ten proteins	1966:1991	the remaining ten proteins	1966:1991	Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant (p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated.					
36248846	9	28	theme	bioinformatic	1518:1530	arg1	analysis					1532:1539	the bioinformatic analysis	1514:1539	the bioinformatic analysis	1514:1539	First, the bioinformatic analysis revealed the composition of the plasma proteome.					
36248846	6	29	theme	strong	995:1000	arg1	pattern					1002:1008	a strong pattern	993:1008	a strong pattern of inflammation that affects systemically and is enough to produce edema in the cavity	993:1095	Nevertheless, they generate a strong pattern of inflammation that affects systemically and is enough to produce edema in the cavity.					
36248846	2	30	theme	blood	504:508	arg1	circulation					510:520	the blood circulation	500:520	the blood circulation	500:520	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	0	31	from	responses	16:24	arg1	inflammation					71:82	zebrafish following λ-carrageenan-Induced inflammation	29:82	zebrafish following λ-carrageenan-Induced inflammation	29:82	Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts.					
36248846	15	32	theme	human	2718:2722	arg1	diseases					2748:2755	human intestinal inflammatory diseases	2718:2755	human intestinal inflammatory diseases	2718:2755	Together, this information can be used for testing and finding novel pharmacological targets to treat human intestinal inflammatory diseases.					
36248846	7	33	from	overexpression	1202:1215	arg1	level					1270:1274	the protein level	1258:1274	the protein level	1258:1274	Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level.					
36248846	1	34	theme	inflammation	181:192	arg1	Regulation					167:176	Regulation	167:176	Regulation of inflammation	167:192	Regulation of inflammation is a critical process for maintaining physiological homeostasis.					
36248846	1	34	theme	inflammation	181:192	arg1	process					208:214	a critical process	197:214	a critical process for maintaining physiological homeostasis	197:256	Regulation of inflammation is a critical process for maintaining physiological homeostasis.					
36248846	14	35	theme	immune	2599:2604	arg1	response					2606:2613	the immune response	2595:2613	the immune response	2595:2613	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	8	36	theme	hybrid	1351:1356	arg1	spectrometry					1379:1390	hybrid linear ion-trap mass spectrometry	1351:1390	a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1349:1417	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	8	37	theme	reversed-phase	1283:1296	arg1	chromatography					1322:1335	reversed-phase high-performance liquid chromatography	1283:1335	reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1283:1417	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	4	38	theme	peritoneal	769:778	arg1	cavity					780:785	the peritoneal cavity	765:785	the peritoneal cavity of adult zebrafish (ZF) (Danio rerio)	765:823	Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio).					
36248846	10	39	theme	control	1672:1678	arg1	groups					1680:1685	the treated and control groups	1656:1685	the treated and control groups	1656:1685	Interestingly, 72 commonly expressed proteins were recorded among the treated and control groups, but, surprisingly, 2830 novel proteins were differentially expressed exclusively in the λ-CGN-induced group.					
36248846	15	40	theme	pharmacological	2685:2699	arg1	targets					2701:2707	novel pharmacological targets	2679:2707	novel pharmacological targets	2679:2707	Together, this information can be used for testing and finding novel pharmacological targets to treat human intestinal inflammatory diseases.					
36248846	0	41	theme	following	39:47	arg1	inflammation					71:82	zebrafish following λ-carrageenan-Induced inflammation	29:82	zebrafish following λ-carrageenan-Induced inflammation	29:82	Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts.					
36248846	2	42	theme	acute	464:468	arg1	reactants					476:484	acute phase reactants	464:484	acute phase reactants secreted into the blood circulation	464:520	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	8	43	theme	spectrometry	1379:1390	arg1	approach					1410:1417	a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1349:1417	a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1349:1417	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	2	44	theme	intestinal	396:405	arg1	inflammation					407:418	acute intestinal inflammation	390:418	acute intestinal inflammation	390:418	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	7	45	theme	notable	1162:1168	arg1	changes					1177:1183	notable tissue changes	1162:1183	notable tissue changes	1162:1183	Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level.					
36248846	9	46	theme	proteome	1580:1587	arg1	composition					1554:1564	the composition	1550:1564	the composition of the plasma proteome	1550:1587	First, the bioinformatic analysis revealed the composition of the plasma proteome.					
36248846	8	47	theme	proteomic	1400:1408	arg1	approach					1410:1417	a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1349:1417	a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1349:1417	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	14	48	theme	proteins	2575:2582	arg1	activation					2457:2466	activation	2457:2466	activation	2457:2466	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	14	48	theme	proteins	2575:2582	arg1	abundance					2472:2480	abundance	2472:2480	abundance	2472:2480	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	11	49	dep	significant	1896:1906	arg1	<					1911:1911	p < 0.05	1909:1916	p < 0.05	1909:1916	Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant (p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated.					
36248846	0	50	theme	zebrafish	29:37	arg1	inflammation					71:82	zebrafish following λ-carrageenan-Induced inflammation	29:82	zebrafish following λ-carrageenan-Induced inflammation	29:82	Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts.					
36248846	2	51	theme	reactants	476:484	arg1	production					450:459	the production	446:459	the production of acute phase reactants secreted into the blood circulation	446:520	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	15	52	dep	testing	2659:2665	arg1	targets					2701:2707	novel pharmacological targets	2679:2707	novel pharmacological targets	2679:2707	Together, this information can be used for testing and finding novel pharmacological targets to treat human intestinal inflammatory diseases.					
36248846	10	53	theme	novel	1712:1716	arg1	proteins					1718:1725	2830 novel proteins	1707:1725	2830 novel proteins	1707:1725	Interestingly, 72 commonly expressed proteins were recorded among the treated and control groups, but, surprisingly, 2830 novel proteins were differentially expressed exclusively in the λ-CGN-induced group.					
36248846	0	54	theme	λ-carrageenan-Induced	49:69	arg1	inflammation					71:82	zebrafish following λ-carrageenan-Induced inflammation	29:82	zebrafish following λ-carrageenan-Induced inflammation	29:82	Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts.					
36248846	14	55	theme	enzymes	2555:2561	arg1	activation					2457:2466	activation	2457:2466	activation	2457:2466	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	14	55	theme	enzymes	2555:2561	arg1	abundance					2472:2480	abundance	2472:2480	abundance	2472:2480	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	8	56	theme	plasma	1439:1444	arg1	proteins					1446:1453	2938 plasma proteins	1434:1453	2938 plasma proteins	1434:1453	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	14	57	theme	several	2537:2543	arg1	enzymes					2555:2561	several catalytic enzymes	2537:2561	several catalytic enzymes	2537:2561	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	12	58	theme	major	2035:2039	arg1	networks					2069:2076	the major protein-protein interaction networks	2031:2076	the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group	2031:2152	Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group.					
36248846	4	59	theme	zebrafish	796:804	arg1	cavity					780:785	the peritoneal cavity	765:785	the peritoneal cavity of adult zebrafish (ZF) (Danio rerio)	765:823	Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio).					
36248846	14	60	theme	reactants	2526:2534	arg1	activation					2457:2466	activation	2457:2466	activation	2457:2466	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	14	60	theme	reactants	2526:2534	arg1	abundance					2472:2480	abundance	2472:2480	abundance	2472:2480	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	7	61	theme	markers	1247:1253	arg1	overexpression					1202:1215	the overexpression	1198:1215	the overexpression of several acute inflammatory markers at the protein level	1198:1274	Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level.					
36248846	14	62	theme	pathway	2425:2431	arg1	analysis					2433:2440	Biological pathway analysis	2414:2440	Biological pathway analysis	2414:2440	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	2	63	theme	capable	370:376	arg1	algae					345:349	red algae	341:349	red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation	341:520	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	2	63	theme	capable	370:376	arg1	crispus					361:367	Chondrus crispus	352:367	Chondrus crispus	352:367	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	14	64	theme	acute	2514:2518	arg1	reactants					2526:2534	diverse classical immune and acute phase reactants	2485:2534	diverse classical immune and acute phase reactants	2485:2534	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	7	65	theme	acute	1228:1232	arg1	markers					1247:1253	several acute inflammatory markers	1220:1253	several acute inflammatory markers at the protein level	1220:1274	Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level.					
36248846	10	66	theme	λ-CGN-induced	1776:1788	arg1	group					1790:1794	the λ-CGN-induced group	1772:1794	the λ-CGN-induced group	1772:1794	Interestingly, 72 commonly expressed proteins were recorded among the treated and control groups, but, surprisingly, 2830 novel proteins were differentially expressed exclusively in the λ-CGN-induced group.					
36248846	14	67	theme	immune	2503:2508	arg1	reactants					2526:2534	diverse classical immune and acute phase reactants	2485:2534	diverse classical immune and acute phase reactants	2485:2534	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	11	68	theme	group	1872:1876	arg1	got					1890:1892	the control group 62 proteins got	1860:1892	the control group 62 proteins got	1860:1892	Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant (p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated.					
36248846	13	69	theme	overlapping	2334:2344	arg1	reconstructions					2349:2363	overlapping 3D reconstructions	2334:2363	overlapping 3D reconstructions between ZF and humans	2334:2385	Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation.					
36248846	14	70	theme	diverse	2485:2491	arg1	reactants					2526:2534	diverse classical immune and acute phase reactants	2485:2534	diverse classical immune and acute phase reactants	2485:2534	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	4	71	theme	Danio	812:816	arg1	zebrafish					796:804	adult zebrafish	790:804	adult zebrafish (ZF) (Danio rerio)	790:823	Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio).					
36248846	4	71	theme	Danio	812:816	arg1	rerio					818:822	Danio rerio	812:822	Danio rerio	812:822	Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio).					
36248846	4	72	theme	λ-CGN-mediated	674:687	arg1	inflammation					689:700	λ-CGN-mediated inflammation	674:700	λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio)	674:823	Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio).					
36248846	10	73	theme	expressed	1617:1625	arg1	proteins					1627:1634	72 commonly expressed proteins	1605:1634	72 commonly expressed proteins	1605:1634	Interestingly, 72 commonly expressed proteins were recorded among the treated and control groups, but, surprisingly, 2830 novel proteins were differentially expressed exclusively in the λ-CGN-induced group.					
36248846	2	74	theme	red	341:343	arg1	algae					345:349	red algae	341:349	red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation	341:520	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	2	74	theme	red	341:343	arg1	crispus					361:367	Chondrus crispus	352:367	Chondrus crispus	352:367	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	1	75	theme	critical	199:206	arg1	Regulation					167:176	Regulation	167:176	Regulation of inflammation	167:192	Regulation of inflammation is a critical process for maintaining physiological homeostasis.					
36248846	1	75	theme	critical	199:206	arg1	process					208:214	a critical process	197:214	a critical process for maintaining physiological homeostasis	197:256	Regulation of inflammation is a critical process for maintaining physiological homeostasis.					
36248846	15	76	used	used	2650:2653	arg2	information					2631:2641	this information	2626:2641	this information	2626:2641	Together, this information can be used for testing and finding novel pharmacological targets to treat human intestinal inflammatory diseases.					
36248846	5	77	contain	had	937:939	arg1	neutrophils					870:880	neutrophils	870:880	neutrophils	870:880	We found that polymorphonuclear leukocytes (neutrophils) and lymphocytes infiltrating the ZF peritoneal cavity had short-term persistence.					
36248846	5	77	contain	had	937:939	arg1	lymphocytes					887:897	lymphocytes	887:897	lymphocytes infiltrating the ZF peritoneal cavity	887:935	We found that polymorphonuclear leukocytes (neutrophils) and lymphocytes infiltrating the ZF peritoneal cavity had short-term persistence.					
36248846	5	77	contain	had	937:939	arg1	leukocytes					858:867	polymorphonuclear leukocytes	840:867	polymorphonuclear leukocytes (neutrophils)	840:881	We found that polymorphonuclear leukocytes (neutrophils) and lymphocytes infiltrating the ZF peritoneal cavity had short-term persistence.					
36248846	5	77	contain	had	937:939	arg2	persistence					952:962	short-term persistence	941:962	short-term persistence	941:962	We found that polymorphonuclear leukocytes (neutrophils) and lymphocytes infiltrating the ZF peritoneal cavity had short-term persistence.					
36248846	12	78	theme	λ-CGN	2134:2138	arg1	group					2148:2152	the λ-CGN induced group	2130:2152	the λ-CGN induced group	2130:2152	Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group.					
36248846	11	79	theme	expressed	1828:1836	arg1	proteins					1838:1845	the commonly expressed proteins	1815:1845	the commonly expressed proteins	1815:1845	Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant (p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated.					
36248846	13	80	theme	similarity	2300:2309	arg1	analysis					2311:2318	a bioinformatic structural similarity analysis	2273:2318	a bioinformatic structural similarity analysis	2273:2318	Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation.					
36248846	1	81	theme	physiological	232:244	arg1	homeostasis					246:256	physiological homeostasis	232:256	physiological homeostasis	232:256	Regulation of inflammation is a critical process for maintaining physiological homeostasis.					
36248846	12	82	theme	blood	2114:2118	arg1	plasma					2120:2125	the blood plasma	2110:2125	the blood plasma of the λ-CGN induced group	2110:2152	Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group.					
36248846	11	83	theme	λ-CGN-treated	1939:1951	arg1	group					1953:1957	the λ-CGN-treated group	1935:1957	the λ-CGN-treated group	1935:1957	Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant (p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated.					
36248846	13	84	theme	bioinformatic	2275:2287	arg1	analysis					2311:2318	a bioinformatic structural similarity analysis	2273:2318	a bioinformatic structural similarity analysis	2273:2318	Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation.					
36248846	6	85	theme	inflammation	1013:1024	arg1	pattern					1002:1008	a strong pattern	993:1008	a strong pattern of inflammation that affects systemically and is enough to produce edema in the cavity	993:1095	Nevertheless, they generate a strong pattern of inflammation that affects systemically and is enough to produce edema in the cavity.					
36248846	8	86	theme	%	1498:1498	arg1	λ-CGN					1500:1504	3.5% λ-CGN	1495:1504	3.5% λ-CGN	1495:1504	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	14	87	dep	activation	2457:2466	arg1	the					2453:2455	the	2453:2455	the	2453:2455	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	12	88	theme	protein	2090:2096	arg1	clusters					2098:2105	hub protein clusters	2086:2105	hub protein clusters in the blood plasma of the λ-CGN induced group	2086:2152	Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group.					
36248846	15	89	theme	intestinal	2724:2733	arg1	diseases					2748:2755	human intestinal inflammatory diseases	2718:2755	human intestinal inflammatory diseases	2718:2755	Together, this information can be used for testing and finding novel pharmacological targets to treat human intestinal inflammatory diseases.					
36248846	7	90	with	Consistent	1098:1107	arg1	findings					1120:1127	these findings	1114:1127	these findings	1114:1127	Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level.					
36248846	13	91	theme	protein	2246:2252	arg1	sets					2254:2257	the unveiled protein sets	2233:2257	the unveiled protein sets	2233:2257	Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation.					
36248846	8	92	theme	liquid	1315:1320	arg1	chromatography					1322:1335	reversed-phase high-performance liquid chromatography	1283:1335	reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1283:1417	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	3	93	from	mechanisms	547:556	arg1	vertebrates					561:571	vertebrates	561:571	vertebrates	561:571	However, the associated mechanisms in vertebrates are not well understood.					
36248846	0	94	theme	PMN	100:102	arg1	leukocytes					104:113	PMN leukocytes	100:113	PMN leukocytes	100:113	Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts.					
36248846	12	95	theme	interaction	2057:2067	arg1	networks					2069:2076	the major protein-protein interaction networks	2031:2076	the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group	2031:2152	Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group.					
36248846	12	96	theme	group	2148:2152	arg1	plasma					2120:2125	the blood plasma	2110:2125	the blood plasma of the λ-CGN induced group	2110:2152	Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group.					
36248846	5	97	theme	short-term	941:950	arg1	persistence					952:962	short-term persistence	941:962	short-term persistence	941:962	We found that polymorphonuclear leukocytes (neutrophils) and lymphocytes infiltrating the ZF peritoneal cavity had short-term persistence.					
36248846	2	98	theme	algae	345:349	arg1	wall					333:336	the cell wall	324:336	the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation	324:520	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	7	99	theme	tissue	1170:1175	arg1	changes					1177:1183	notable tissue changes	1162:1183	notable tissue changes	1162:1183	Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level.					
36248846	8	100	theme	linear	1358:1363	arg1	spectrometry					1379:1390	hybrid linear ion-trap mass spectrometry	1351:1390	a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1349:1417	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	15	101	theme	novel	2679:2683	arg1	targets					2701:2707	novel pharmacological targets	2679:2707	novel pharmacological targets	2679:2707	Together, this information can be used for testing and finding novel pharmacological targets to treat human intestinal inflammatory diseases.					
36248846	0	102	theme	proteome	7:14	arg1	responses					16:24	Plasma proteome responses	0:24	Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation	0:82	Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts.					
36248846	5	103	theme	ZF	916:917	arg1	cavity					930:935	the ZF peritoneal cavity	912:935	the ZF peritoneal cavity	912:935	We found that polymorphonuclear leukocytes (neutrophils) and lymphocytes infiltrating the ZF peritoneal cavity had short-term persistence.					
36248846	8	104	theme	mass	1374:1377	arg1	spectrometry					1379:1390	hybrid linear ion-trap mass spectrometry	1351:1390	a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1349:1417	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	2	105	theme	acute	390:394	arg1	inflammation					407:418	acute intestinal inflammation	390:418	acute intestinal inflammation	390:418	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	8	106	theme	ion-trap	1365:1372	arg1	spectrometry					1379:1390	hybrid linear ion-trap mass spectrometry	1351:1390	a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1349:1417	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	8	107	theme	shotgun	1392:1398	arg1	approach					1410:1417	a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1349:1417	a hybrid linear ion-trap mass spectrometry shotgun proteomic approach	1349:1417	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	7	108	theme	cell	1130:1133	arg1	infiltration					1135:1146	cell infiltration	1130:1146	cell infiltration	1130:1146	Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level.					
36248846	13	109	theme	unveiled	2237:2244	arg1	sets					2254:2257	the unveiled protein sets	2233:2257	the unveiled protein sets	2233:2257	Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation.					
36248846	14	110	theme	varied	2568:2573	arg1	proteins					2575:2582	varied proteins	2568:2582	varied proteins supporting the immune response	2568:2613	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	13	111	theme	acute	2394:2398	arg1	inflammation					2400:2411	acute inflammation	2394:2411	acute inflammation	2394:2411	Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation.					
36248846	7	112	theme	protein	1262:1268	arg1	level					1270:1274	the protein level	1258:1274	the protein level	1258:1274	Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level.					
36248846	2	113	theme	phase	470:474	arg1	reactants					476:484	acute phase reactants	464:484	acute phase reactants secreted into the blood circulation	464:520	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	2	114	theme	Chondrus	352:359	arg1	algae					345:349	red algae	341:349	red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation	341:520	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	2	114	theme	Chondrus	352:359	arg1	crispus					361:367	Chondrus crispus	352:367	Chondrus crispus	352:367	The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation.					
36248846	13	115	theme	effects	2216:2222	arg1	underpinnings					2193:2205	the molecular underpinnings	2179:2205	the molecular underpinnings of these effects based on the unveiled protein sets	2179:2257	Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation.					
36248846	14	116	theme	catalytic	2545:2553	arg1	enzymes					2555:2561	several catalytic enzymes	2537:2561	several catalytic enzymes	2537:2561	Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response.					
36248846	4	117	theme	crucial	624:630	arg1	factors					632:638	the crucial factors	620:638	the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio)	620:823	Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio).					
36248846	8	118	theme	2938	1434:1437	arg1	proteins					1446:1453	2938 plasma proteins	1434:1453	2938 plasma proteins	1434:1453	Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN.					
36248846	11	119	theme	control	1864:1870	arg1	got					1890:1892	the control group 62 proteins got	1860:1892	the control group 62 proteins got	1860:1892	Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant (p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated.					
36248846	7	120	theme	inflammatory	1234:1245	arg1	markers					1247:1253	several acute inflammatory markers	1220:1253	several acute inflammatory markers at the protein level	1220:1274	Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level.					
36248846	4	121	theme	adult	790:794	arg1	ZF					807:808	ZF	807:808	ZF	807:808	Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio).					
36248846	4	121	theme	adult	790:794	arg1	zebrafish					796:804	adult zebrafish	790:804	adult zebrafish (ZF) (Danio rerio)	790:823	Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio).					
36248846	4	121	theme	adult	790:794	arg1	rerio					818:822	Danio rerio	812:822	Danio rerio	812:822	Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio).					
36248846	3	122	theme	associated	536:545	arg1	mechanisms					547:556	the associated mechanisms	532:556	the associated mechanisms in vertebrates	532:571	However, the associated mechanisms in vertebrates are not well understood.					
36248846	13	123	theme	molecular	2183:2191	arg1	underpinnings					2193:2205	the molecular underpinnings	2179:2205	the molecular underpinnings of these effects based on the unveiled protein sets	2179:2257	Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation.					
36017369	9	0	theme	Forest	1555:1560	arg1	analysis					1562:1569	a Random Forest analysis	1546:1569	a Random Forest analysis with 10-fold cross-validation	1546:1599	Further, a Random Forest analysis with 10-fold cross-validation identified a family named Muribaculaceae representing colitis development and recovery upon LbGP treatment.					
36017369	5	1	from	mechanism	1065:1073	arg1	treatment					1030:1038	the treatment	1026:1038	the treatment of acute colitis	1026:1055	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	9	2	with	analysis	1562:1569	arg1	cross-validation					1584:1599	10-fold cross-validation	1576:1599	10-fold cross-validation	1576:1599	Further, a Random Forest analysis with 10-fold cross-validation identified a family named Muribaculaceae representing colitis development and recovery upon LbGP treatment.					
36017369	1	3	theme	acute	234:238	arg1	colitis					240:246	acute colitis	234:246	acute colitis	234:246	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	10	4	theme	microbiota	1869:1878	arg1	diversity					1848:1856	diversity	1848:1856	diversity	1848:1856	In conclusion, our study demonstrated the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression.					
36017369	10	4	theme	microbiota	1869:1878	arg1	composition					1832:1842	composition	1832:1842	composition	1832:1842	In conclusion, our study demonstrated the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression.					
36017369	1	5	theme	most	165:168	arg1	cases					170:174	most cases	165:174	most cases	165:174	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	4	6	theme	therapeutic	796:806	arg1	efficacy					808:815	its therapeutic efficacy	792:815	its therapeutic efficacy in colitis	792:826	However, its therapeutic efficacy in colitis and its mechanism in gut microbiota regulation have not been fully studied.					
36017369	5	7	used	used	975:978	arg2	model					965:969	the dextran sulfate sodium (DSS)-induced mouse model	918:969	the dextran sulfate sodium (DSS)-induced mouse model	918:969	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	1	8	theme	human	381:385	arg1	body					387:390	the human body	377:390	the human body	377:390	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	7	9	theme	Harmful	1298:1304	arg1	spp					1342:1344	Lachnoclostridium spp	1324:1344	Lachnoclostridium spp	1324:1344	Harmful bacteria, such as Lachnoclostridium spp.					
36017369	7	9	theme	Harmful	1298:1304	arg1	bacteria					1306:1313	Harmful bacteria	1298:1313	Harmful bacteria	1298:1313	Harmful bacteria, such as Lachnoclostridium spp.					
36017369	10	10	theme	dynamic	1900:1906	arg1	process					1908:1914	the dynamic process	1896:1914	the dynamic process of gut microbiota with the colitis progression	1896:1961	In conclusion, our study demonstrated the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression.					
36017369	6	11	theme	gut	1244:1246	arg1	microbiome					1248:1257	the gut microbiome	1240:1257	the gut microbiome	1240:1257	The results showed that LbGP treatment significantly alleviated acute colitis and improved the gut microbiome compared with that in the model group.					
36017369	1	12	theme	largest	350:356	arg1	microbiome					325:334	the gut microbiome	317:334	the gut microbiome	317:334	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	1	12	theme	largest	350:356	arg1	micro-ecosystem					358:372	the largest micro-ecosystem	346:372	the largest micro-ecosystem in the human body	346:390	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	1	13	theme	chronic	187:193	arg1	colitis					195:201	recurrent chronic colitis	177:201	recurrent chronic colitis	177:201	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	5	14	theme	gut	1103:1105	arg1	microbiome					1107:1116	the gut microbiome	1099:1116	the gut microbiome	1099:1116	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	11	15	theme	food	2026:2029	arg1	LbGP					2005:2008	LbGP	2005:2008	LbGP	2005:2008	Further, it provides evidence to develop LbGP as a functional food supplement and future drug acting on intestinal disease.					
36017369	11	15	theme	food	2026:2029	arg1	supplement					2031:2040	a functional food supplement	2013:2040	a functional food supplement	2013:2040	Further, it provides evidence to develop LbGP as a functional food supplement and future drug acting on intestinal disease.					
36017369	10	16	theme	microbiota	1923:1932	arg1	process					1908:1914	the dynamic process	1896:1914	the dynamic process of gut microbiota with the colitis progression	1896:1961	In conclusion, our study demonstrated the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression.					
36017369	0	17	theme	microbiota	142:151	arg1	diversity					121:129	diversity	121:129	diversity	121:129	Lycium barbarum Glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice.					
36017369	0	17	theme	microbiota	142:151	arg1	composition					105:115	composition	105:115	composition	105:115	Lycium barbarum Glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice.					
36017369	5	18	theme	mouse	959:963	arg1	model					965:969	the dextran sulfate sodium (DSS)-induced mouse model	918:969	the dextran sulfate sodium (DSS)-induced mouse model	918:969	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	4	19	theme	gut	849:851	arg1	regulation					864:873	gut microbiota regulation	849:873	gut microbiota regulation	849:873	However, its therapeutic efficacy in colitis and its mechanism in gut microbiota regulation have not been fully studied.					
36017369	0	20	from	composition	105:115	arg1	mice					156:159	mice	156:159	mice	156:159	Lycium barbarum Glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice.					
36017369	4	21	from	mechanism	836:844	arg1	regulation					864:873	gut microbiota regulation	849:873	gut microbiota regulation	849:873	However, its therapeutic efficacy in colitis and its mechanism in gut microbiota regulation have not been fully studied.					
36017369	4	21	from	mechanism	836:844	arg1	colitis					820:826	colitis	820:826	colitis	820:826	However, its therapeutic efficacy in colitis and its mechanism in gut microbiota regulation have not been fully studied.					
36017369	9	22	theme	LbGP	1693:1696	arg1	treatment					1698:1706	LbGP treatment	1693:1706	LbGP treatment	1693:1706	Further, a Random Forest analysis with 10-fold cross-validation identified a family named Muribaculaceae representing colitis development and recovery upon LbGP treatment.					
36017369	5	23	theme	microbiome	1107:1116	arg1	perspective					1084:1094	the perspective	1080:1094	the perspective of the gut microbiome through the 16S rDNA sequence	1080:1146	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	10	24	theme	LbGP	1765:1768	arg1	capability					1751:1760	the capability	1747:1760	the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression	1747:1961	In conclusion, our study demonstrated the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression.					
36017369	2	25	theme	Plant	445:449	arg1	polysaccharides					451:465	Plant polysaccharides	445:465	Plant polysaccharides	445:465	Plant polysaccharides have always been reported to have the ability for anti-inflammation, and they are closely related to the gut microbiome.					
36017369	3	26	from	fruit	719:723	arg1	purification					685:696	purification	685:696	purification	685:696	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	3	26	from	fruit	719:723	arg1	isolation					671:679	further isolation	663:679	further isolation	663:679	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	0	27	theme	colitis	79:85	arg1	development					42:52	development	42:52	development	42:52	Lycium barbarum Glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice.					
36017369	0	27	theme	colitis	79:85	arg1	progression					58:68	progression	58:68	progression	58:68	Lycium barbarum Glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice.					
36017369	1	28	theme	gut	321:323	arg1	microbiome					325:334	the gut microbiome	317:334	the gut microbiome	317:334	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	1	28	theme	gut	321:323	arg1	micro-ecosystem					358:372	the largest micro-ecosystem	346:372	the largest micro-ecosystem in the human body	346:390	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	11	29	theme	future	2046:2051	arg1	drug					2053:2056	future drug	2046:2056	future drug	2046:2056	Further, it provides evidence to develop LbGP as a functional food supplement and future drug acting on intestinal disease.					
36017369	0	30	dep	composition	105:115	arg1	the					101:103	the	101:103	the	101:103	Lycium barbarum Glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice.					
36017369	1	31	from	micro-ecosystem	358:372	arg1	body					387:390	the human body	377:390	the human body	377:390	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	5	32	theme	LbGP	1018:1021	arg1	effect					1008:1013	the effect	1004:1013	the effect of LbGP in the treatment of acute colitis	1004:1055	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	5	32	theme	LbGP	1018:1021	arg1	mechanism					1065:1073	the mechanism	1061:1073	the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence	1061:1146	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	0	33	theme	Lycium	0:5	arg1	Glycopeptide					16:27	Lycium barbarum Glycopeptide	0:27	Lycium barbarum Glycopeptide	0:27	Lycium barbarum Glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice.					
36017369	1	34	theme	incomplete	254:263	arg1	recovery					265:272	incomplete recovery	254:272	incomplete recovery	254:272	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	10	35	theme	acute	1800:1804	arg1	colitis					1806:1812	acute colitis	1800:1812	acute colitis	1800:1812	In conclusion, our study demonstrated the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression.					
36017369	5	36	theme	colitis	1049:1055	arg1	treatment					1030:1038	the treatment	1026:1038	the treatment of acute colitis	1026:1055	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	3	37	theme	barbarum	595:602	arg1	Glycopeptide					604:615	Lycium barbarum Glycopeptide	588:615	Lycium barbarum Glycopeptide (LbGP)	588:622	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	3	37	theme	barbarum	595:602	arg1	component					641:649	the most potent component	625:649	the most potent component obtained by further isolation and purification from Lycium barbarum fruit	625:723	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	3	37	theme	barbarum	595:602	arg1	LbGP					618:621	LbGP	618:621	LbGP	618:621	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	5	38	from	treatment	1030:1038	arg1	effect					1008:1013	the effect	1004:1013	the effect of LbGP in the treatment of acute colitis	1004:1055	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	5	38	from	treatment	1030:1038	arg1	mechanism					1065:1073	the mechanism	1061:1073	the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence	1061:1146	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	5	39	theme	-induced	950:957	arg1	model					965:969	the dextran sulfate sodium (DSS)-induced mouse model	918:969	the dextran sulfate sodium (DSS)-induced mouse model	918:969	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	8	40	dep	Turicibacter	1464:1475	arg1	spp.					1477:1480	Turicibacter spp.	1464:1480	Turicibacter spp.	1464:1480	and Parabacteroides_distasonis, were inhibited and probiotics, such as Bacteroides_acidifaciens, Lactobacillus spp., Turicibacter spp., and Alistipes spp., were increased by LbGP treatment.					
36017369	5	41	theme	rDNA	1134:1137	arg1	sequence					1139:1146	the 16S rDNA sequence	1126:1146	the 16S rDNA sequence	1126:1146	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	9	42	theme	Random	1548:1553	arg1	analysis					1562:1569	a Random Forest analysis	1546:1569	a Random Forest analysis with 10-fold cross-validation	1546:1599	Further, a Random Forest analysis with 10-fold cross-validation identified a family named Muribaculaceae representing colitis development and recovery upon LbGP treatment.					
36017369	0	43	from	diversity	121:129	arg1	mice					156:159	mice	156:159	mice	156:159	Lycium barbarum Glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice.					
36017369	10	44	theme	gut	1865:1867	arg1	microbiota					1869:1878	the gut microbiota	1861:1878	the gut microbiota	1861:1878	In conclusion, our study demonstrated the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression.					
36017369	2	45	theme	gut	572:574	arg1	microbiome					576:585	the gut microbiome	568:585	the gut microbiome	568:585	Plant polysaccharides have always been reported to have the ability for anti-inflammation, and they are closely related to the gut microbiome.					
36017369	3	46	theme	potent	634:639	arg1	component					641:649	the most potent component	625:649	the most potent component obtained by further isolation and purification from Lycium barbarum fruit	625:723	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	3	46	theme	potent	634:639	arg1	Glycopeptide					604:615	Lycium barbarum Glycopeptide	588:615	Lycium barbarum Glycopeptide (LbGP)	588:622	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	6	47	theme	LbGP	1173:1176	arg1	treatment					1178:1186	LbGP treatment	1173:1186	LbGP treatment	1173:1186	The results showed that LbGP treatment significantly alleviated acute colitis and improved the gut microbiome compared with that in the model group.					
36017369	1	48	theme	crucial	401:407	arg1	role					409:412	a crucial role	399:412	a crucial role	399:412	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	1	49	theme	colitis	240:246	arg1	recurrence					220:229	the recurrence	216:229	the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors	216:310	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	9	50	theme	10-fold	1576:1582	arg1	cross-validation					1584:1599	10-fold cross-validation	1576:1599	10-fold cross-validation	1576:1599	Further, a Random Forest analysis with 10-fold cross-validation identified a family named Muribaculaceae representing colitis development and recovery upon LbGP treatment.					
36017369	5	51	from	perspective	1084:1094	arg1	effect					1008:1013	the effect	1004:1013	the effect of LbGP in the treatment of acute colitis	1004:1055	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	5	51	from	perspective	1084:1094	arg1	mechanism					1065:1073	the mechanism	1061:1073	the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence	1061:1146	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	7	52	theme	Lachnoclostridium	1324:1340	arg1	spp					1342:1344	Lachnoclostridium spp	1324:1344	Lachnoclostridium spp	1324:1344	Harmful bacteria, such as Lachnoclostridium spp.					
36017369	3	53	theme	further	663:669	arg1	isolation					671:679	further isolation	663:679	further isolation	663:679	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	3	54	theme	Lycium	703:708	arg1	fruit					719:723	Lycium barbarum fruit	703:723	Lycium barbarum fruit	703:723	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	3	55	dep	Lycium	703:708	arg1	barbarum					710:717	barbarum	710:717	barbarum	710:717	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	11	56	theme	intestinal	2068:2077	arg1	disease					2079:2085	intestinal disease	2068:2085	intestinal disease	2068:2085	Further, it provides evidence to develop LbGP as a functional food supplement and future drug acting on intestinal disease.					
36017369	1	57	theme	colitis	436:442	arg1	development					421:431	the development	417:431	the development of colitis	417:442	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	1	58	theme	recurrent	177:185	arg1	colitis					195:201	recurrent chronic colitis	177:201	recurrent chronic colitis	177:201	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	4	59	theme	microbiota	853:862	arg1	regulation					864:873	gut microbiota regulation	849:873	gut microbiota regulation	849:873	However, its therapeutic efficacy in colitis and its mechanism in gut microbiota regulation have not been fully studied.					
36017369	0	60	theme	gut	138:140	arg1	microbiota					142:151	the gut microbiota	134:151	the gut microbiota	134:151	Lycium barbarum Glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice.					
36017369	9	61	theme	colitis	1655:1661	arg1	development					1663:1673	colitis development	1655:1673	colitis development	1655:1673	Further, a Random Forest analysis with 10-fold cross-validation identified a family named Muribaculaceae representing colitis development and recovery upon LbGP treatment.					
36017369	5	62	theme	acute	1043:1047	arg1	colitis					1049:1055	acute colitis	1043:1055	acute colitis	1043:1055	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	10	63	with	process	1908:1914	arg1	progression					1951:1961	the colitis progression	1939:1961	the colitis progression	1939:1961	In conclusion, our study demonstrated the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression.					
36017369	10	64	theme	gut	1919:1921	arg1	microbiota					1923:1932	gut microbiota	1919:1932	gut microbiota	1919:1932	In conclusion, our study demonstrated the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression.					
36017369	6	65	theme	acute	1213:1217	arg1	colitis					1219:1225	acute colitis	1213:1225	acute colitis	1213:1225	The results showed that LbGP treatment significantly alleviated acute colitis and improved the gut microbiome compared with that in the model group.					
36017369	11	66	theme	functional	2015:2024	arg1	LbGP					2005:2008	LbGP	2005:2008	LbGP	2005:2008	Further, it provides evidence to develop LbGP as a functional food supplement and future drug acting on intestinal disease.					
36017369	11	66	theme	functional	2015:2024	arg1	supplement					2031:2040	a functional food supplement	2013:2040	a functional food supplement	2013:2040	Further, it provides evidence to develop LbGP as a functional food supplement and future drug acting on intestinal disease.					
36017369	8	67	dep	Alistipes	1487:1495	arg1	spp.					1497:1500	Alistipes spp.	1487:1500	Alistipes spp.	1487:1500	and Parabacteroides_distasonis, were inhibited and probiotics, such as Bacteroides_acidifaciens, Lactobacillus spp., Turicibacter spp., and Alistipes spp., were increased by LbGP treatment.					
36017369	0	68	theme	acute	73:77	arg1	colitis					79:85	acute colitis	73:85	acute colitis	73:85	Lycium barbarum Glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice.					
36017369	10	69	theme	colitis	1943:1949	arg1	progression					1951:1961	the colitis progression	1939:1961	the colitis progression	1939:1961	In conclusion, our study demonstrated the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression.					
36017369	6	70	theme	model	1285:1289	arg1	group					1291:1295	the model group	1281:1295	the model group	1281:1295	The results showed that LbGP treatment significantly alleviated acute colitis and improved the gut microbiome compared with that in the model group.					
36017369	4	71	from	efficacy	808:815	arg1	regulation					864:873	gut microbiota regulation	849:873	gut microbiota regulation	849:873	However, its therapeutic efficacy in colitis and its mechanism in gut microbiota regulation have not been fully studied.					
36017369	4	71	from	efficacy	808:815	arg1	colitis					820:826	colitis	820:826	colitis	820:826	However, its therapeutic efficacy in colitis and its mechanism in gut microbiota regulation have not been fully studied.					
36017369	2	72	contain	have	496:499	arg1	polysaccharides					451:465	Plant polysaccharides	445:465	Plant polysaccharides	445:465	Plant polysaccharides have always been reported to have the ability for anti-inflammation, and they are closely related to the gut microbiome.					
36017369	2	72	contain	have	496:499	arg2	ability					505:511	the ability	501:511	the ability for anti-inflammation	501:533	Plant polysaccharides have always been reported to have the ability for anti-inflammation, and they are closely related to the gut microbiome.					
36017369	3	73	theme	animal	768:773	arg1	models					775:780	animal models	768:780	animal models	768:780	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	0	74	theme	barbarum	7:14	arg1	Glycopeptide					16:27	Lycium barbarum Glycopeptide	0:27	Lycium barbarum Glycopeptide	0:27	Lycium barbarum Glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice.					
36017369	5	75	from	effect	1008:1013	arg1	treatment					1030:1038	the treatment	1026:1038	the treatment of acute colitis	1026:1055	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	10	76	theme	colitis	1806:1812	arg1	development					1785:1795	the development	1781:1795	the development of acute colitis	1781:1812	In conclusion, our study demonstrated the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression.					
36017369	3	77	theme	Lycium	588:593	arg1	Glycopeptide					604:615	Lycium barbarum Glycopeptide	588:615	Lycium barbarum Glycopeptide (LbGP)	588:622	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	3	77	theme	Lycium	588:593	arg1	component					641:649	the most potent component	625:649	the most potent component obtained by further isolation and purification from Lycium barbarum fruit	625:723	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	3	77	theme	Lycium	588:593	arg1	LbGP					618:621	LbGP	618:621	LbGP	618:621	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	1	78	theme	irritating	293:302	arg1	factors					304:310	irritating factors	293:310	irritating factors	293:310	In most cases, recurrent chronic colitis is caused by the recurrence of acute colitis after incomplete recovery and re-exposure to irritating factors, and the gut microbiome, which is the largest micro-ecosystem in the human body, plays a crucial role in the development of colitis.					
36017369	0	79	dep	development	42:52	arg1	the					38:40	the	38:40	the	38:40	Lycium barbarum Glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice.					
36017369	8	80	theme	LbGP	1521:1524	arg1	treatment					1526:1534	LbGP treatment	1521:1534	LbGP treatment	1521:1534	and Parabacteroides_distasonis, were inhibited and probiotics, such as Bacteroides_acidifaciens, Lactobacillus spp., Turicibacter spp., and Alistipes spp., were increased by LbGP treatment.					
36017369	3	81	from	inflammation	752:763	arg1	models					775:780	animal models	768:780	animal models	768:780	Lycium barbarum Glycopeptide (LbGP), the most potent component obtained by further isolation and purification from Lycium barbarum fruit, has been shown to inhibit inflammation in animal models.					
36017369	5	82	theme	16S	1130:1132	arg1	sequence					1139:1146	the 16S rDNA sequence	1126:1146	the 16S rDNA sequence	1126:1146	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	10	83	dep	composition	1832:1842	arg1	the					1828:1830	the	1828:1830	the	1828:1830	In conclusion, our study demonstrated the capability of LbGP to prevent the development of acute colitis by regulating the composition and diversity of the gut microbiota and highlighted the dynamic process of gut microbiota with the colitis progression.					
36017369	5	84	from	LbGP	1018:1021	arg1	treatment					1030:1038	the treatment	1026:1038	the treatment of acute colitis	1026:1055	In our study, the dextran sulfate sodium (DSS)-induced mouse model was used to dynamically evaluate the effect of LbGP in the treatment of acute colitis and the mechanism from the perspective of the gut microbiome through the 16S rDNA sequence.					
36017369	8	85	dep	Lactobacillus	1444:1456	arg1	spp.					1458:1461	Lactobacillus spp.	1444:1461	Lactobacillus spp.	1444:1461	and Parabacteroides_distasonis, were inhibited and probiotics, such as Bacteroides_acidifaciens, Lactobacillus spp., Turicibacter spp., and Alistipes spp., were increased by LbGP treatment.					
35593392	7	0	theme	anti-obesity	1602:1613	arg1	effects					1615:1621	the anti-obesity effects	1598:1621	the anti-obesity effects of FucLj	1598:1630	Overall, mice with different initial gut microbiota responded differently to FucLj supplementation on a high-fat diet, and metronidazole-sensitive gut bacteria negatively correlated with obesity symptoms and were required for the anti-obesity effects of FucLj.					
35593392	6	1	theme	fat	1164:1166	arg1	accumulation					1168:1179	fat accumulation	1164:1179	fat accumulation	1164:1179	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	9	2	theme	personalized	2011:2022	arg1	way					2024:2026	a personalized way	2009:2026	a personalized way	2009:2026	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	4	3	theme	present	598:604	arg1	study					606:610	the present study	594:610	the present study	594:610	In the present study, the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole were compared and investigated.					
35593392	9	4	from	application	1974:1984	arg1	way					2024:2026	a personalized way	2009:2026	a personalized way	2009:2026	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	3	5	from	supplementation	509:523	arg1	models					541:546	HFD-fed mice models	528:546	HFD-fed mice models	528:546	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	2	6	theme	large	345:349	arg1	variability					368:378	large inter-individual variability	345:378	large inter-individual variability in gut microbiota	345:396	However, the outcome of dietary intervention may differ between individuals due to large inter-individual variability in gut microbiota.					
35593392	1	7	theme	microbiota	250:259	arg1	composition					231:241	the composition	227:241	the composition of gut microbiota	227:259	Previous studies have indicated that fucoidan could resist high-fat-diet (HFD)-induced obesity by modulating the composition of gut microbiota.					
35593392	6	8	theme	metronidazole-treated	1344:1364	arg1	mice					1366:1369	metronidazole-treated mice	1344:1369	metronidazole-treated mice	1344:1369	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	6	9	from	gain	1158:1161	arg1	mice					1304:1307	penicillin-treated and untreated mice	1271:1307	penicillin-treated and untreated mice	1271:1307	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	3	10	theme	gut	563:565	arg1	communities					578:588	different gut microbiota communities	553:588	different gut microbiota communities	553:588	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	7	11	theme	initial	1401:1407	arg1	microbiota					1413:1422	different initial gut microbiota	1391:1422	different initial gut microbiota	1391:1422	Overall, mice with different initial gut microbiota responded differently to FucLj supplementation on a high-fat diet, and metronidazole-sensitive gut bacteria negatively correlated with obesity symptoms and were required for the anti-obesity effects of FucLj.					
35593392	5	12	theme	metronidazole-treated	900:920	arg1	groups					933:938	penicillin-treated, metronidazole-treated and normal groups	880:938	penicillin-treated, metronidazole-treated and normal groups	880:938	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	7	13	theme	metronidazole-sensitive	1495:1517	arg1	bacteria					1523:1530	metronidazole-sensitive gut bacteria	1495:1530	metronidazole-sensitive gut bacteria	1495:1530	Overall, mice with different initial gut microbiota responded differently to FucLj supplementation on a high-fat diet, and metronidazole-sensitive gut bacteria negatively correlated with obesity symptoms and were required for the anti-obesity effects of FucLj.					
35593392	5	14	theme	normal	926:931	arg1	groups					933:938	penicillin-treated, metronidazole-treated and normal groups	880:938	penicillin-treated, metronidazole-treated and normal groups	880:938	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	9	15	theme	microbiota	1802:1811	arg1	structure					1813:1821	the gut microbiota structure	1794:1821	the gut microbiota structure before dietary interventions	1794:1850	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	2	16	from	variability	368:378	arg1	microbiota					387:396	gut microbiota	383:396	gut microbiota	383:396	However, the outcome of dietary intervention may differ between individuals due to large inter-individual variability in gut microbiota.					
35593392	7	17	with	mice	1381:1384	arg1	microbiota					1413:1422	different initial gut microbiota	1391:1422	different initial gut microbiota	1391:1422	Overall, mice with different initial gut microbiota responded differently to FucLj supplementation on a high-fat diet, and metronidazole-sensitive gut bacteria negatively correlated with obesity symptoms and were required for the anti-obesity effects of FucLj.					
35593392	2	18	theme	dietary	286:292	arg1	intervention					294:305	dietary intervention	286:305	dietary intervention	286:305	However, the outcome of dietary intervention may differ between individuals due to large inter-individual variability in gut microbiota.					
35593392	5	19	theme	microbiota	863:872	arg1	differences					844:854	the differences	840:854	the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups	840:938	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	7	20	theme	high-fat	1476:1483	arg1	diet					1485:1488	a high-fat diet	1474:1488	a high-fat diet	1474:1488	Overall, mice with different initial gut microbiota responded differently to FucLj supplementation on a high-fat diet, and metronidazole-sensitive gut bacteria negatively correlated with obesity symptoms and were required for the anti-obesity effects of FucLj.					
35593392	6	21	theme	HFD-induced	1134:1144	arg1	gain					1158:1161	HFD-induced body weight gain	1134:1161	HFD-induced body weight gain	1134:1161	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	5	22	theme	penicillin-treated	880:897	arg1	groups					933:938	penicillin-treated, metronidazole-treated and normal groups	880:938	penicillin-treated, metronidazole-treated and normal groups	880:938	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	1	23	theme	Previous	118:125	arg1	studies					127:133	Previous studies	118:133	Previous studies	118:133	Previous studies have indicated that fucoidan could resist high-fat-diet (HFD)-induced obesity by modulating the composition of gut microbiota.					
35593392	6	24	theme	untreated	1294:1302	arg1	mice					1304:1307	penicillin-treated and untreated mice	1271:1307	penicillin-treated and untreated mice	1271:1307	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	7	25	theme	FucLj	1449:1453	arg1	supplementation					1455:1469	FucLj supplementation	1449:1469	FucLj supplementation	1449:1469	Overall, mice with different initial gut microbiota responded differently to FucLj supplementation on a high-fat diet, and metronidazole-sensitive gut bacteria negatively correlated with obesity symptoms and were required for the anti-obesity effects of FucLj.					
35593392	6	26	theme	penicillin-treated	1271:1288	arg1	mice					1304:1307	penicillin-treated and untreated mice	1271:1307	penicillin-treated and untreated mice	1271:1307	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	4	27	theme	normal	694:699	arg1	mice					701:704	normal mice	694:704	normal mice	694:704	In the present study, the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole were compared and investigated.					
35593392	4	28	from	effects	630:636	arg1	mice					701:704	normal mice	694:704	normal mice	694:704	In the present study, the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole were compared and investigated.					
35593392	4	28	from	effects	630:636	arg1	mice					729:732	microbiota-altered mice	710:732	microbiota-altered mice	710:732	In the present study, the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole were compared and investigated.					
35593392	9	29	theme	beneficial	1881:1890	arg1	outcomes					1892:1899	the beneficial outcomes	1877:1899	the beneficial outcomes of dietary fiber supplementation	1877:1932	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	6	30	located	observed	1332:1339	arg1	mice					1366:1369	metronidazole-treated mice	1344:1369	metronidazole-treated mice	1344:1369	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	6	30	located	observed	1332:1339	arg2	effects					1319:1325	no effects	1316:1325	no effects	1316:1325	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	9	31	theme	fiber	1912:1916	arg1	supplementation					1918:1932	dietary fiber supplementation	1904:1932	dietary fiber supplementation	1904:1932	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	6	32	from	steatosis	1232:1240	arg1	mice					1304:1307	penicillin-treated and untreated mice	1271:1307	penicillin-treated and untreated mice	1271:1307	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	0	33	theme	microbiota	96:105	arg1	structure					107:115	the gut microbiota structure	88:115	the gut microbiota structure	88:115	Anti-obesity effects of Laminaria japonica fucoidan in high-fat diet-fed mice vary with the gut microbiota structure.					
35593392	5	34	theme	phylum	1030:1035	arg1	Bacteroidetes					1037:1049	the phylum Bacteroidetes	1026:1049	the phylum Bacteroidetes	1026:1049	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	6	35	theme	weight	1151:1156	arg1	gain					1158:1161	HFD-induced body weight gain	1134:1161	HFD-induced body weight gain	1134:1161	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	0	36	theme	Anti-obesity	0:11	arg1	effects					13:19	Anti-obesity effects	0:19	Anti-obesity effects of Laminaria japonica fucoidan in high-fat diet-fed mice	0:76	Anti-obesity effects of Laminaria japonica fucoidan in high-fat diet-fed mice vary with the gut microbiota structure.					
35593392	7	37	theme	gut	1409:1411	arg1	microbiota					1413:1422	different initial gut microbiota	1391:1422	different initial gut microbiota	1391:1422	Overall, mice with different initial gut microbiota responded differently to FucLj supplementation on a high-fat diet, and metronidazole-sensitive gut bacteria negatively correlated with obesity symptoms and were required for the anti-obesity effects of FucLj.					
35593392	5	38	theme	relative	1004:1011	arg1	abundance					1013:1021	greater relative abundance	996:1021	greater relative abundance of the phylum Bacteroidetes	996:1049	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	7	39	theme	different	1391:1399	arg1	microbiota					1413:1422	different initial gut microbiota	1391:1422	different initial gut microbiota	1391:1422	Overall, mice with different initial gut microbiota responded differently to FucLj supplementation on a high-fat diet, and metronidazole-sensitive gut bacteria negatively correlated with obesity symptoms and were required for the anti-obesity effects of FucLj.					
35593392	3	40	theme	present	409:415	arg1	study					417:421	the present study	405:421	the present study	405:421	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	3	41	theme	possible	448:455	arg1	discrepancy					457:467	the possible discrepancy	444:467	the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities	444:588	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	0	42	theme	japonica	34:41	arg1	fucoidan					43:50	Laminaria japonica fucoidan	24:50	Laminaria japonica fucoidan	24:50	Anti-obesity effects of Laminaria japonica fucoidan in high-fat diet-fed mice vary with the gut microbiota structure.					
35593392	5	43	theme	16S	811:813	arg1	sequencing					820:829	The 16S rRNA sequencing	807:829	The 16S rRNA sequencing	807:829	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	9	44	theme	further	1966:1972	arg1	application					1974:1984	the further application	1962:1984	the further application of dietary fucoidan in a personalized way	1962:2026	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	0	45	theme	diet-fed	64:71	arg1	mice					73:76	high-fat diet-fed mice	55:76	high-fat diet-fed mice	55:76	Anti-obesity effects of Laminaria japonica fucoidan in high-fat diet-fed mice vary with the gut microbiota structure.					
35593392	7	46	theme	FucLj	1626:1630	arg1	effects					1615:1621	the anti-obesity effects	1598:1621	the anti-obesity effects of FucLj	1598:1630	Overall, mice with different initial gut microbiota responded differently to FucLj supplementation on a high-fat diet, and metronidazole-sensitive gut bacteria negatively correlated with obesity symptoms and were required for the anti-obesity effects of FucLj.					
35593392	4	47	attach	isolated	650:657	arg2	fucoidan					641:648	fucoidan	641:648	fucoidan isolated from Laminaria japonica (FucLj)	641:689	In the present study, the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole were compared and investigated.					
35593392	4	47	attach	isolated	650:657	arg1	Laminaria					664:672	Laminaria	664:672	Laminaria	664:672	In the present study, the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole were compared and investigated.					
35593392	4	47	attach	isolated	650:657	arg1	FucLj					684:688	FucLj	684:688	FucLj	684:688	In the present study, the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole were compared and investigated.					
35593392	6	48	from	profiles	1194:1201	arg1	mice					1304:1307	penicillin-treated and untreated mice	1271:1307	penicillin-treated and untreated mice	1271:1307	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	3	49	theme	anti-obesity	476:487	arg1	effects					489:495	the anti-obesity effects	472:495	the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities	472:588	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	6	50	theme	serum	1182:1186	arg1	profiles					1194:1201	serum lipid profiles	1182:1201	serum lipid profiles	1182:1201	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	9	51	theme	fucoidan	1997:2004	arg1	application					1974:1984	the further application	1962:1984	the further application of dietary fucoidan in a personalized way	1962:2026	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	8	52	theme	FucLj	1709:1713	arg1	utilization					1694:1704	the utilization	1690:1704	the utilization of FucLj by gut microbiota to produce SCFAs	1690:1748	Moreover, the anti-obesity effects were not dependent on the utilization of FucLj by gut microbiota to produce SCFAs.					
35593392	3	53	theme	HFD-fed	528:534	arg1	models					541:546	HFD-fed mice models	528:546	HFD-fed mice models	528:546	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	0	54	theme	high-fat	55:62	arg1	mice					73:76	high-fat diet-fed mice	55:76	high-fat diet-fed mice	55:76	Anti-obesity effects of Laminaria japonica fucoidan in high-fat diet-fed mice vary with the gut microbiota structure.					
35593392	8	55	theme	gut	1718:1720	arg1	microbiota					1722:1731	gut microbiota	1718:1731	gut microbiota	1718:1731	Moreover, the anti-obesity effects were not dependent on the utilization of FucLj by gut microbiota to produce SCFAs.					
35593392	3	56	with	supplementation	509:523	arg1	communities					578:588	different gut microbiota communities	553:588	different gut microbiota communities	553:588	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	6	57	from	accumulation	1168:1179	arg1	mice					1304:1307	penicillin-treated and untreated mice	1271:1307	penicillin-treated and untreated mice	1271:1307	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	2	58	theme	inter-individual	351:366	arg1	variability					368:378	large inter-individual variability	345:378	large inter-individual variability in gut microbiota	345:396	However, the outcome of dietary intervention may differ between individuals due to large inter-individual variability in gut microbiota.					
35593392	1	59	theme	gut	246:248	arg1	microbiota					250:259	gut microbiota	246:259	gut microbiota	246:259	Previous studies have indicated that fucoidan could resist high-fat-diet (HFD)-induced obesity by modulating the composition of gut microbiota.					
35593392	1	60	theme	-induced	196:203	arg1	obesity					205:211	high-fat-diet (HFD)-induced obesity	177:211	high-fat-diet (HFD)-induced obesity	177:211	Previous studies have indicated that fucoidan could resist high-fat-diet (HFD)-induced obesity by modulating the composition of gut microbiota.					
35593392	3	61	theme	different	553:561	arg1	communities					578:588	different gut microbiota communities	553:588	different gut microbiota communities	553:588	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	6	62	theme	hepatic	1224:1230	arg1	steatosis					1232:1240	hepatic steatosis	1224:1240	hepatic steatosis	1224:1240	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	6	63	theme	adipocyte	1246:1254	arg1	hypertrophy					1256:1266	adipocyte hypertrophy	1246:1266	adipocyte hypertrophy	1246:1266	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	7	64	theme	obesity	1559:1565	arg1	symptoms					1567:1574	obesity symptoms	1559:1574	obesity symptoms	1559:1574	Overall, mice with different initial gut microbiota responded differently to FucLj supplementation on a high-fat diet, and metronidazole-sensitive gut bacteria negatively correlated with obesity symptoms and were required for the anti-obesity effects of FucLj.					
35593392	3	65	from	effects	489:495	arg1	models					541:546	HFD-fed mice models	528:546	HFD-fed mice models	528:546	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	9	66	theme	gut	1798:1800	arg1	structure					1813:1821	the gut microbiota structure	1794:1821	the gut microbiota structure before dietary interventions	1794:1850	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	7	67	theme	gut	1519:1521	arg1	bacteria					1523:1530	metronidazole-sensitive gut bacteria	1495:1530	metronidazole-sensitive gut bacteria	1495:1530	Overall, mice with different initial gut microbiota responded differently to FucLj supplementation on a high-fat diet, and metronidazole-sensitive gut bacteria negatively correlated with obesity symptoms and were required for the anti-obesity effects of FucLj.					
35593392	2	68	theme	intervention	294:305	arg1	outcome					275:281	the outcome	271:281	the outcome of dietary intervention	271:305	However, the outcome of dietary intervention may differ between individuals due to large inter-individual variability in gut microbiota.					
35593392	5	69	theme	gut	859:861	arg1	microbiota					863:872	gut microbiota	859:872	gut microbiota	859:872	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	9	70	theme	structure	1813:1821	arg1	evaluation					1780:1789	evaluation	1780:1789	evaluation of the gut microbiota structure before dietary interventions	1780:1850	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	6	71	theme	insulin	1204:1210	arg1	resistance					1212:1221	insulin resistance	1204:1221	insulin resistance	1204:1221	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	4	72	theme	fucoidan	641:648	arg1	effects					630:636	the anti-obesity effects	613:636	the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole	613:773	In the present study, the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole were compared and investigated.					
35593392	3	73	theme	supplementation	509:523	arg1	effects					489:495	the anti-obesity effects	472:495	the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities	472:588	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	3	74	theme	fucoidan	500:507	arg1	supplementation					509:523	fucoidan supplementation	500:523	fucoidan supplementation in HFD-fed mice models with different gut microbiota communities	500:588	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	9	75	theme	dietary	1830:1836	arg1	interventions					1838:1850	dietary interventions	1830:1850	dietary interventions	1830:1850	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	4	76	theme	microbiota-altered	710:727	arg1	mice					729:732	microbiota-altered mice	710:732	microbiota-altered mice	710:732	In the present study, the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole were compared and investigated.					
35593392	3	77	theme	microbiota	567:576	arg1	communities					578:588	different gut microbiota communities	553:588	different gut microbiota communities	553:588	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	3	78	from	models	541:546	arg1	effects					489:495	the anti-obesity effects	472:495	the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities	472:588	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	0	79	from	effects	13:19	arg1	mice					73:76	high-fat diet-fed mice	55:76	high-fat diet-fed mice	55:76	Anti-obesity effects of Laminaria japonica fucoidan in high-fat diet-fed mice vary with the gut microbiota structure.					
35593392	0	80	theme	gut	92:94	arg1	structure					107:115	the gut microbiota structure	88:115	the gut microbiota structure	88:115	Anti-obesity effects of Laminaria japonica fucoidan in high-fat diet-fed mice vary with the gut microbiota structure.					
35593392	9	81	theme	dietary	1904:1910	arg1	supplementation					1918:1932	dietary fiber supplementation	1904:1932	dietary fiber supplementation	1904:1932	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	5	82	dep	families	1059:1066	arg1	families					1059:1066	the families	1055:1066	the families Muribaculaceae and Bacteroidaceae	1055:1100	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	5	82	dep	families	1059:1066	arg1	Bacteroidaceae					1087:1100	Bacteroidaceae	1087:1100	Bacteroidaceae	1087:1100	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	5	82	dep	families	1059:1066	arg1	Muribaculaceae					1068:1081	Muribaculaceae	1068:1081	Muribaculaceae	1068:1081	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	9	83	theme	supplementation	1918:1932	arg1	outcomes					1892:1899	the beneficial outcomes	1877:1899	the beneficial outcomes of dietary fiber supplementation	1877:1932	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	8	84	theme	anti-obesity	1647:1658	arg1	effects					1660:1666	the anti-obesity effects	1643:1666	the anti-obesity effects	1643:1666	Moreover, the anti-obesity effects were not dependent on the utilization of FucLj by gut microbiota to produce SCFAs.					
35593392	8	84	theme	anti-obesity	1647:1658	arg1	dependent					1677:1685	dependent	1677:1685	dependent	1677:1685	Moreover, the anti-obesity effects were not dependent on the utilization of FucLj by gut microbiota to produce SCFAs.					
35593392	6	85	from	hypertrophy	1256:1266	arg1	mice					1304:1307	penicillin-treated and untreated mice	1271:1307	penicillin-treated and untreated mice	1271:1307	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	6	86	theme	body	1146:1149	arg1	gain					1158:1161	HFD-induced body weight gain	1134:1161	HFD-induced body weight gain	1134:1161	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	6	87	from	resistance	1212:1221	arg1	mice					1304:1307	penicillin-treated and untreated mice	1271:1307	penicillin-treated and untreated mice	1271:1307	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	2	88	theme	gut	383:385	arg1	microbiota					387:396	gut microbiota	383:396	gut microbiota	383:396	However, the outcome of dietary intervention may differ between individuals due to large inter-individual variability in gut microbiota.					
35593392	0	89	theme	Laminaria	24:32	arg1	fucoidan					43:50	Laminaria japonica fucoidan	24:50	Laminaria japonica fucoidan	24:50	Anti-obesity effects of Laminaria japonica fucoidan in high-fat diet-fed mice vary with the gut microbiota structure.					
35593392	5	90	theme	greater	996:1002	arg1	abundance					1013:1021	greater relative abundance	996:1021	greater relative abundance of the phylum Bacteroidetes	996:1049	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	0	91	theme	fucoidan	43:50	arg1	effects					13:19	Anti-obesity effects	0:19	Anti-obesity effects of Laminaria japonica fucoidan in high-fat diet-fed mice	0:76	Anti-obesity effects of Laminaria japonica fucoidan in high-fat diet-fed mice vary with the gut microbiota structure.					
35593392	4	92	dep	Laminaria	664:672	arg1	japonica					674:681	Laminaria japonica	664:681	Laminaria japonica (FucLj)	664:689	In the present study, the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole were compared and investigated.					
35593392	5	93	theme	rRNA	815:818	arg1	sequencing					820:829	The 16S rRNA sequencing	807:829	The 16S rRNA sequencing	807:829	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	2	94	theme	due	338:340	arg1	individuals					326:336	individuals	326:336	individuals due to large inter-individual variability in gut microbiota	326:396	However, the outcome of dietary intervention may differ between individuals due to large inter-individual variability in gut microbiota.					
35593392	5	95	theme	Bacteroidetes	1037:1049	arg1	families					1059:1066	the families	1055:1066	the families Muribaculaceae and Bacteroidaceae	1055:1100	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	5	95	theme	Bacteroidetes	1037:1049	arg1	Bacteroidaceae					1087:1100	Bacteroidaceae	1087:1100	Bacteroidaceae	1087:1100	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	5	95	theme	Bacteroidetes	1037:1049	arg1	Muribaculaceae					1068:1081	Muribaculaceae	1068:1081	Muribaculaceae	1068:1081	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	5	95	theme	Bacteroidetes	1037:1049	arg1	abundance					1013:1021	greater relative abundance	996:1021	greater relative abundance of the phylum Bacteroidetes	996:1049	The 16S rRNA sequencing revealed the differences of gut microbiota among penicillin-treated, metronidazole-treated and normal groups, and mice treated with penicillin were characterized by greater relative abundance of the phylum Bacteroidetes and the families Muribaculaceae and Bacteroidaceae.					
35593392	6	96	theme	lipid	1188:1192	arg1	profiles					1194:1201	serum lipid profiles	1182:1201	serum lipid profiles	1182:1201	Furthermore, FucLj ameliorated HFD-induced body weight gain, fat accumulation, serum lipid profiles, insulin resistance, hepatic steatosis and adipocyte hypertrophy in penicillin-treated and untreated mice, while no effects were observed in metronidazole-treated mice.					
35593392	9	97	theme	dietary	1989:1995	arg1	fucoidan					1997:2004	dietary fucoidan	1989:2004	dietary fucoidan	1989:2004	These findings indicate that evaluation of the gut microbiota structure before dietary interventions is helpful for enhancing the beneficial outcomes of dietary fiber supplementation and provide a rationale for the further application of dietary fucoidan in a personalized way.					
35593392	4	98	theme	anti-obesity	617:628	arg1	effects					630:636	the anti-obesity effects	613:636	the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole	613:773	In the present study, the anti-obesity effects of fucoidan isolated from Laminaria japonica (FucLj) on normal mice and microbiota-altered mice treated with penicillin or metronidazole were compared and investigated.					
35593392	3	99	theme	effects	489:495	arg1	discrepancy					457:467	the possible discrepancy	444:467	the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities	444:588	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
35593392	3	100	theme	mice	536:539	arg1	models					541:546	HFD-fed mice models	528:546	HFD-fed mice models	528:546	Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.					
36669633	9	0	theme	composition	949:959	arg1	study					937:941	the study	933:941	the study of LG composition and toxicity	933:972	These results demonstrate advance in the study of LG composition and toxicity, indicating a potential for several biomedical and biotechnological future applications.					
36669633	7	1	theme	prostate	685:692	arg1	cells					716:720	human prostate adenocarcinoma cancer cells	679:720	human prostate adenocarcinoma cancer cells	679:720	LG showed antiproliferative activity against human prostate adenocarcinoma cancer cells, with percentage superior to 50 %.					
36669633	5	2	theme	isolate	544:550	arg1	purity					552:557	the isolate purity	540:557	the isolate purity	540:557	Small traces of protein were detected, emphasizing the isolate purity.					
36669633	7	3	theme	superior	739:746	arg1	percentage					728:737	percentage	728:737	percentage superior to 50 %	728:754	LG showed antiproliferative activity against human prostate adenocarcinoma cancer cells, with percentage superior to 50 %.					
36669633	0	4	theme	prostate	116:123	arg1	cells					140:144	human prostate adenocarcinoma cells	110:144	human prostate adenocarcinoma cells	110:144	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.					
36669633	6	5	theme	different	604:612	arg1	events					626:631	three different degradation events	598:631	three different degradation events	598:631	Molar mass was 8.08 × 105 g/mol, with three different degradation events.					
36669633	8	6	theme	control	881:887	arg1	group					889:893	control group	881:893	control group	881:893	In vivo toxicity models demonstrated that LG is biocompatible polymer, with little difference in the parameters compared to control group.					
36669633	8	7	theme	little	833:838	arg1	difference					840:849	little difference	833:849	little difference in the parameters	833:867	In vivo toxicity models demonstrated that LG is biocompatible polymer, with little difference in the parameters compared to control group.					
36669633	0	8	theme	human	110:114	arg1	cells					140:144	human prostate adenocarcinoma cells	110:144	human prostate adenocarcinoma cells	110:144	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.					
36669633	7	9	theme	antiproliferative	644:660	arg1	activity					662:669	antiproliferative activity	644:669	antiproliferative activity against human prostate adenocarcinoma cancer cells	644:720	LG showed antiproliferative activity against human prostate adenocarcinoma cancer cells, with percentage superior to 50 %.					
36669633	5	10	theme	protein	505:511	arg1	traces					495:500	Small traces	489:500	Small traces of protein	489:511	Small traces of protein were detected, emphasizing the isolate purity.					
36669633	1	11	theme	Citrus	176:181	arg1	latifolia					185:193	Citrus × latifolia	176:193	Citrus × latifolia in Brazil	176:203	Lemon gum (LG) obtained from Citrus × latifolia in Brazil was isolated and characterized.					
36669633	0	12	theme	adenocarcinoma	125:138	arg1	cells					140:144	human prostate adenocarcinoma cells	110:144	human prostate adenocarcinoma cells	110:144	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.					
36669633	6	13	with	8.08 × 105 g/mol	575:590	arg1	events					626:631	three different degradation events	598:631	three different degradation events	598:631	Molar mass was 8.08 × 105 g/mol, with three different degradation events.					
36669633	6	14	theme	degradation	614:624	arg1	events					626:631	three different degradation events	598:631	three different degradation events	598:631	Molar mass was 8.08 × 105 g/mol, with three different degradation events.					
36669633	1	15	theme	×	183:183	arg1	latifolia					185:193	Citrus × latifolia	176:193	Citrus × latifolia in Brazil	176:203	Lemon gum (LG) obtained from Citrus × latifolia in Brazil was isolated and characterized.					
36669633	0	16	attach	isolated	37:44	arg2	arabinogalactan					21:35	Non-toxic arabinogalactan	11:35	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.	0:145	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.					
36669633	0	16	attach	isolated	37:44	arg1	Citrus × latifolia					51:68	Citrus × latifolia	51:68	Citrus × latifolia	51:68	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.					
36669633	2	17	theme	Galleria	309:316	arg1	mellonella					318:327	Galleria mellonella	309:327	Galleria mellonella	309:327	In addition, gum biocompatibility was evaluated in vitro and in vivo by Galleria mellonella and mice model.					
36669633	0	18	theme	Lemon	0:4	arg1	gum					6:8	Lemon gum	0:8	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.	0:145	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.					
36669633	2	19	dep	mellonella	318:327	arg1	model					338:342	model	338:342	model	338:342	In addition, gum biocompatibility was evaluated in vitro and in vivo by Galleria mellonella and mice model.					
36669633	3	20	theme	tumor	370:374	arg1	cells					376:380	tumor cells	370:380	tumor cells	370:380	The cytotoxicity against tumor cells was also evaluated.					
36669633	2	21	theme	gum	250:252	arg1	biocompatibility					254:269	gum biocompatibility	250:269	gum biocompatibility	250:269	In addition, gum biocompatibility was evaluated in vitro and in vivo by Galleria mellonella and mice model.					
36669633	8	22	theme	In	757:758	arg1	models					774:779	In vivo toxicity models	757:779	In vivo toxicity models	757:779	In vivo toxicity models demonstrated that LG is biocompatible polymer, with little difference in the parameters compared to control group.					
36669633	4	23	theme	rhamnose:4-OMe-glucuronic	436:460	arg1	acid					462:465	rhamnose:4-OMe-glucuronic acid	436:465	The ratio of arabinose:galactose: rhamnose:4-OMe-glucuronic acid	402:465	The ratio of arabinose:galactose: rhamnose:4-OMe-glucuronic acid was 1:0.65:0.06:0.15.					
36669633	8	24	with	polymer	819:825	arg1	difference					840:849	little difference	833:849	little difference in the parameters	833:867	In vivo toxicity models demonstrated that LG is biocompatible polymer, with little difference in the parameters compared to control group.					
36669633	5	25	theme	Small	489:493	arg1	traces					495:500	Small traces	489:500	Small traces of protein	489:511	Small traces of protein were detected, emphasizing the isolate purity.					
36669633	9	26	theme	biotechnological	1025:1040	arg1	applications					1049:1060	several biomedical and biotechnological future applications	1002:1060	applications	1049:1060	These results demonstrate advance in the study of LG composition and toxicity, indicating a potential for several biomedical and biotechnological future applications.					
36669633	8	27	theme	toxicity	765:772	arg1	models					774:779	In vivo toxicity models	757:779	In vivo toxicity models	757:779	In vivo toxicity models demonstrated that LG is biocompatible polymer, with little difference in the parameters compared to control group.					
36669633	8	28	dep	In	757:758	arg1	vivo					760:763	vivo	760:763	vivo	760:763	In vivo toxicity models demonstrated that LG is biocompatible polymer, with little difference in the parameters compared to control group.					
36669633	0	29	theme	Non-toxic	11:19	arg1	arabinogalactan					21:35	Non-toxic arabinogalactan	11:35	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.	0:145	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.					
36669633	9	30	theme	future	1042:1047	arg1	applications					1049:1060	several biomedical and biotechnological future applications	1002:1060	applications	1049:1060	These results demonstrate advance in the study of LG composition and toxicity, indicating a potential for several biomedical and biotechnological future applications.					
36669633	9	31	theme	toxicity	965:972	arg1	study					937:941	the study	933:941	the study of LG composition and toxicity	933:972	These results demonstrate advance in the study of LG composition and toxicity, indicating a potential for several biomedical and biotechnological future applications.					
36669633	9	32	theme	LG	946:947	arg1	composition					949:959	LG composition	946:959	LG composition	946:959	These results demonstrate advance in the study of LG composition and toxicity, indicating a potential for several biomedical and biotechnological future applications.					
36669633	4	33	theme	arabinose	415:423	arg1	1:0.65:0.06:0.15					471:486	1:0.65:0.06:0.15	471:486	1:0.65:0.06:0.15	471:486	The ratio of arabinose:galactose: rhamnose:4-OMe-glucuronic acid was 1:0.65:0.06:0.15.					
36669633	4	33	theme	arabinose	415:423	arg1	ratio					406:410	The ratio	402:410	The ratio of arabinose:galactose: rhamnose:4-OMe-glucuronic acid	402:465	The ratio of arabinose:galactose: rhamnose:4-OMe-glucuronic acid was 1:0.65:0.06:0.15.					
36669633	1	34	theme	Lemon	147:151	arg1	gum					153:155	Lemon gum	147:155	Lemon gum (LG) obtained from Citrus × latifolia in Brazil	147:203	Lemon gum (LG) obtained from Citrus × latifolia in Brazil was isolated and characterized.					
36669633	1	34	theme	Lemon	147:151	arg1	LG					158:159	LG	158:159	LG	158:159	Lemon gum (LG) obtained from Citrus × latifolia in Brazil was isolated and characterized.					
36669633	8	35	from	difference	840:849	arg1	parameters					858:867	the parameters	854:867	the parameters	854:867	In vivo toxicity models demonstrated that LG is biocompatible polymer, with little difference in the parameters compared to control group.					
36669633	7	36	theme	adenocarcinoma	694:707	arg1	cells					716:720	human prostate adenocarcinoma cancer cells	679:720	human prostate adenocarcinoma cancer cells	679:720	LG showed antiproliferative activity against human prostate adenocarcinoma cancer cells, with percentage superior to 50 %.					
36669633	1	37	from	latifolia	185:193	arg1	Brazil					198:203	Brazil	198:203	Brazil	198:203	Lemon gum (LG) obtained from Citrus × latifolia in Brazil was isolated and characterized.					
36669633	0	38	theme	antiproliferative	75:91	arg1	property					93:100	antiproliferative property	75:100	antiproliferative property against human prostate adenocarcinoma cells	75:144	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.					
36669633	7	39	theme	cancer	709:714	arg1	cells					716:720	human prostate adenocarcinoma cancer cells	679:720	human prostate adenocarcinoma cancer cells	679:720	LG showed antiproliferative activity against human prostate adenocarcinoma cancer cells, with percentage superior to 50 %.					
36669633	0	40	dep	gum	6:8	arg1	arabinogalactan					21:35	Non-toxic arabinogalactan	11:35	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.	0:145	Lemon gum: Non-toxic arabinogalactan isolated from Citrus × latifolia with antiproliferative property against human prostate adenocarcinoma cells.					
36669633	8	41	theme	biocompatible	805:817	arg1	polymer					819:825	biocompatible polymer	805:825	biocompatible polymer	805:825	In vivo toxicity models demonstrated that LG is biocompatible polymer, with little difference in the parameters compared to control group.					
36669633	6	42	theme	Molar	560:564	arg1	mass					566:569	Molar mass	560:569	Molar mass	560:569	Molar mass was 8.08 × 105 g/mol, with three different degradation events.					
36669633	7	43	theme	human	679:683	arg1	cells					716:720	human prostate adenocarcinoma cancer cells	679:720	human prostate adenocarcinoma cancer cells	679:720	LG showed antiproliferative activity against human prostate adenocarcinoma cancer cells, with percentage superior to 50 %.					
36669633	4	44	dep	ratio	406:410	arg1	galactose					425:433	galactose	425:433	The ratio of arabinose:galactose: rhamnose:4-OMe-glucuronic acid	402:465	The ratio of arabinose:galactose: rhamnose:4-OMe-glucuronic acid was 1:0.65:0.06:0.15.					
36669633	4	44	dep	ratio	406:410	arg1	acid					462:465	rhamnose:4-OMe-glucuronic acid	436:465	The ratio of arabinose:galactose: rhamnose:4-OMe-glucuronic acid	402:465	The ratio of arabinose:galactose: rhamnose:4-OMe-glucuronic acid was 1:0.65:0.06:0.15.					
35868209	0	0	theme	assisted	99:106	arg1	reaction					117:124	assisted Maillard reaction	99:124	assisted Maillard reaction	99:124	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	3	1	theme	secondary	535:543	arg1	structure					545:553	secondary structure	535:553	secondary structure	535:553	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
35868209	1	2	theme	functional	271:280	arg1	properties					282:291	interface and functional properties	257:291	interface and functional properties	257:291	Jiuzao glutelin isolate (JGI) was reported to possess interface and functional properties.					
35868209	2	3	theme	assisted	427:434	arg1	reaction					445:452	ultrasound-stirring assisted Maillard reaction	407:452	ultrasound-stirring assisted Maillard reaction (UTSA-MR)	407:462	To enhance the stability and properties of JGI, conjugation between JGI and carboxymethyl chitosan (CTS) through ultrasound-stirring assisted Maillard reaction (UTSA-MR) was investigated and optimized.					
35868209	2	3	theme	assisted	427:434	arg1	UTSA-MR					455:461	UTSA-MR	455:461	UTSA-MR	455:461	To enhance the stability and properties of JGI, conjugation between JGI and carboxymethyl chitosan (CTS) through ultrasound-stirring assisted Maillard reaction (UTSA-MR) was investigated and optimized.					
35868209	2	4	theme	carboxymethyl	370:382	arg1	chitosan					384:391	carboxymethyl chitosan	370:391	carboxymethyl chitosan (CTS)	370:397	To enhance the stability and properties of JGI, conjugation between JGI and carboxymethyl chitosan (CTS) through ultrasound-stirring assisted Maillard reaction (UTSA-MR) was investigated and optimized.					
35868209	2	4	theme	carboxymethyl	370:382	arg1	CTS					394:396	CTS	394:396	CTS	394:396	To enhance the stability and properties of JGI, conjugation between JGI and carboxymethyl chitosan (CTS) through ultrasound-stirring assisted Maillard reaction (UTSA-MR) was investigated and optimized.					
35868209	8	5	theme	potential	1590:1598	arg1	conjugate					1600:1608	a potential conjugate	1588:1608	a potential conjugate with functions that can be used to encapsulate functional substances in the stabilized nano-emulsion	1588:1709	CTS-JGI-2 is a potential conjugate with functions that can be used to encapsulate functional substances in the stabilized nano-emulsion.					
35868209	8	5	theme	potential	1590:1598	arg1	CTS-JGI-2					1575:1583	CTS-JGI-2	1575:1583	CTS-JGI-2	1575:1583	CTS-JGI-2 is a potential conjugate with functions that can be used to encapsulate functional substances in the stabilized nano-emulsion.					
35868209	0	6	from	nano-emulsion	188:200	arg1	reaction					117:124	assisted Maillard reaction	99:124	assisted Maillard reaction	99:124	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	0	6	from	nano-emulsion	188:200	arg1	effect					145:150	its protective effect	130:150	its protective effect of loading resveratrol/quercetin in nano-emulsion	130:200	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	6	7	dep	stabilized	1080:1089	arg1	%					1304:1304	77.94%	1299:1304	77.94% for QUE	1299:1312	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	6	7	dep	stabilized	1080:1089	arg1	%					1285:1285	73.23%	1280:1285	73.23% for RES	1280:1293	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	5	8	theme	emulsifying	948:958	arg1	ability					960:966	emulsifying ability	948:966	emulsifying ability	948:966	Under the optimal glycation, the conjugate (CTS/JGI, 2:1, w/w; CTS-JGI-2) exhibited the best emulsifying ability and stability against NaCl solution, in vitro antioxidant activity, and cholesterol-lowering ability.					
35868209	5	9	theme	NaCl	990:993	arg1	solution					995:1002	NaCl solution	990:1002	NaCl solution	990:1002	Under the optimal glycation, the conjugate (CTS/JGI, 2:1, w/w; CTS-JGI-2) exhibited the best emulsifying ability and stability against NaCl solution, in vitro antioxidant activity, and cholesterol-lowering ability.					
35868209	3	10	theme	amino	572:576	arg1	composition					583:593	amino acid composition	572:593	amino acid composition of JGI	572:600	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
35868209	4	11	theme	weight	759:764	arg1	ratios					766:771	weight ratios	759:771	weight ratios of 0.5:1	759:780	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	7	12	theme	JGI	1457:1459	arg1	method					1523:1528	an excellent method	1510:1528	an excellent method to improve the functional properties of JGI	1510:1572	Taken together, the modification of JGI by conjugating with CTS through UTSA-MR could be an excellent method to improve the functional properties of JGI.					
35868209	7	12	theme	JGI	1457:1459	arg1	modification					1441:1452	the modification	1437:1452	the modification of JGI by conjugating with CTS through UTSA-MR	1437:1499	Taken together, the modification of JGI by conjugating with CTS through UTSA-MR could be an excellent method to improve the functional properties of JGI.					
35868209	0	13	theme	Maillard	108:115	arg1	reaction					117:124	assisted Maillard reaction	99:124	assisted Maillard reaction	99:124	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	8	14	with	conjugate	1600:1608	arg1	functions					1615:1623	functions	1615:1623	functions that can be used to encapsulate functional substances in the stabilized nano-emulsion	1615:1709	CTS-JGI-2 is a potential conjugate with functions that can be used to encapsulate functional substances in the stabilized nano-emulsion.					
35868209	3	15	theme	acid	578:581	arg1	composition					583:593	amino acid composition	572:593	amino acid composition of JGI	572:600	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
35868209	6	16	dep	efficiency	1179:1188	arg1	%					1215:1215	93.13%	1210:1215	93.13% for QUE	1210:1223	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	6	16	dep	efficiency	1179:1188	arg1	%					1196:1196	80.96%	1191:1196	80.96% for RES	1191:1204	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	0	17	from	resveratrol/quercetin	163:183	arg1	nano-emulsion					188:200	nano-emulsion	188:200	nano-emulsion	188:200	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	3	18	with	conjugation	622:632	arg1	CTS					639:641	CTS	639:641	CTS	639:641	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
35868209	4	19	theme	conjugates	733:742	arg1	stability					715:723	thermal stability	707:723	thermal stability	707:723	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	4	19	theme	conjugates	733:742	arg1	property					668:675	foaming property	660:675	foaming property	660:675	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	4	19	theme	conjugates	733:742	arg1	viscosity					692:700	viscosity	692:700	viscosity	692:700	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	4	19	theme	conjugates	733:742	arg1	stability					681:689	stability	681:689	stability	681:689	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	4	19	theme	conjugates	733:742	arg1	solubility					648:657	solubility	648:657	solubility	648:657	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	1	20	theme	Jiuzao	203:208	arg1	isolate					219:225	Jiuzao glutelin isolate	203:225	Jiuzao glutelin isolate (JGI)	203:231	Jiuzao glutelin isolate (JGI) was reported to possess interface and functional properties.					
35868209	1	20	theme	Jiuzao	203:208	arg1	JGI					228:230	JGI	228:230	JGI	228:230	Jiuzao glutelin isolate (JGI) was reported to possess interface and functional properties.					
35868209	5	21	dep	in	1005:1006	arg1	vitro					1008:1012	vitro	1008:1012	vitro	1008:1012	Under the optimal glycation, the conjugate (CTS/JGI, 2:1, w/w; CTS-JGI-2) exhibited the best emulsifying ability and stability against NaCl solution, in vitro antioxidant activity, and cholesterol-lowering ability.					
35868209	8	22	used	used	1637:1640	arg2	functions					1615:1623	functions	1615:1623	functions that can be used to encapsulate functional substances in the stabilized nano-emulsion	1615:1709	CTS-JGI-2 is a potential conjugate with functions that can be used to encapsulate functional substances in the stabilized nano-emulsion.					
35868209	6	23	theme	quercetin	1149:1157	arg1	efficiency					1179:1188	oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency	1091:1188	oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE)	1091:1224	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	1	24	theme	glutelin	210:217	arg1	isolate					219:225	Jiuzao glutelin isolate	203:225	Jiuzao glutelin isolate (JGI)	203:231	Jiuzao glutelin isolate (JGI) was reported to possess interface and functional properties.					
35868209	1	24	theme	glutelin	210:217	arg1	JGI					228:230	JGI	228:230	JGI	228:230	Jiuzao glutelin isolate (JGI) was reported to possess interface and functional properties.					
35868209	4	25	theme	native	843:848	arg1	JGI					850:852	native JGI	843:852	native JGI	843:852	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	3	26	theme	JGI	598:600	arg1	distribution					521:532	molecular distribution	511:532	molecular distribution	511:532	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
35868209	3	26	theme	JGI	598:600	arg1	structure					545:553	secondary structure	535:553	secondary structure	535:553	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
35868209	3	26	theme	JGI	598:600	arg1	composition					583:593	amino acid composition	572:593	amino acid composition of JGI	572:600	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
35868209	3	26	theme	JGI	598:600	arg1	morphology					556:565	morphology	556:565	morphology	556:565	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
35868209	6	27	theme	digestion	1261:1269	arg1	process					1271:1277	the simulated digestion process	1247:1277	the simulated digestion process	1247:1277	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	2	28	theme	JGI	337:339	arg1	stability					309:317	stability	309:317	stability	309:317	To enhance the stability and properties of JGI, conjugation between JGI and carboxymethyl chitosan (CTS) through ultrasound-stirring assisted Maillard reaction (UTSA-MR) was investigated and optimized.					
35868209	2	28	theme	JGI	337:339	arg1	properties					323:332	properties	323:332	properties	323:332	To enhance the stability and properties of JGI, conjugation between JGI and carboxymethyl chitosan (CTS) through ultrasound-stirring assisted Maillard reaction (UTSA-MR) was investigated and optimized.					
35868209	7	29	theme	JGI	1570:1572	arg1	properties					1556:1565	the functional properties	1541:1565	the functional properties of JGI	1541:1572	Taken together, the modification of JGI by conjugating with CTS through UTSA-MR could be an excellent method to improve the functional properties of JGI.					
35868209	0	30	theme	Jiuzao	24:29	arg1	glutelin					31:38	modified Jiuzao glutelin	15:38	modified Jiuzao glutelin	15:38	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	7	31	theme	functional	1545:1554	arg1	properties					1556:1565	the functional properties	1541:1565	the functional properties of JGI	1541:1572	Taken together, the modification of JGI by conjugating with CTS through UTSA-MR could be an excellent method to improve the functional properties of JGI.					
35868209	3	32	theme	composition	583:593	arg1	changes					500:506	The changes	496:506	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI	496:600	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
35868209	4	33	theme	foaming	660:666	arg1	property					668:675	foaming property	660:675	foaming property	660:675	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	0	34	theme	modified	15:22	arg1	glutelin					31:38	modified Jiuzao glutelin	15:38	modified Jiuzao glutelin	15:38	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	0	35	theme	protective	134:143	arg1	effect					145:150	its protective effect	130:150	its protective effect of loading resveratrol/quercetin in nano-emulsion	130:200	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	2	36	dep	stability	309:317	arg1	the					305:307	the	305:307	the	305:307	To enhance the stability and properties of JGI, conjugation between JGI and carboxymethyl chitosan (CTS) through ultrasound-stirring assisted Maillard reaction (UTSA-MR) was investigated and optimized.					
35868209	5	37	theme	optimal	865:871	arg1	glycation					873:881	the optimal glycation	861:881	the optimal glycation	861:881	Under the optimal glycation, the conjugate (CTS/JGI, 2:1, w/w; CTS-JGI-2) exhibited the best emulsifying ability and stability against NaCl solution, in vitro antioxidant activity, and cholesterol-lowering ability.					
35868209	0	38	theme	loading	155:161	arg1	resveratrol/quercetin					163:183	loading resveratrol/quercetin	155:183	loading resveratrol/quercetin in nano-emulsion	155:200	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	0	39	theme	glutelin	31:38	arg1	Preparation					0:10	Preparation	0:10	Preparation of modified Jiuzao glutelin	0:38	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	7	40	theme	excellent	1513:1521	arg1	method					1523:1528	an excellent method	1510:1528	an excellent method to improve the functional properties of JGI	1510:1572	Taken together, the modification of JGI by conjugating with CTS through UTSA-MR could be an excellent method to improve the functional properties of JGI.					
35868209	7	40	theme	excellent	1513:1521	arg1	modification					1441:1452	the modification	1437:1452	the modification of JGI by conjugating with CTS through UTSA-MR	1437:1499	Taken together, the modification of JGI by conjugating with CTS through UTSA-MR could be an excellent method to improve the functional properties of JGI.					
35868209	0	41	from	effect	145:150	arg1	nano-emulsion					188:200	nano-emulsion	188:200	nano-emulsion	188:200	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	8	42	theme	functional	1657:1666	arg1	substances					1668:1677	functional substances	1657:1677	functional substances	1657:1677	CTS-JGI-2 is a potential conjugate with functions that can be used to encapsulate functional substances in the stabilized nano-emulsion.					
35868209	2	43	theme	Maillard	436:443	arg1	reaction					445:452	ultrasound-stirring assisted Maillard reaction	407:452	ultrasound-stirring assisted Maillard reaction (UTSA-MR)	407:462	To enhance the stability and properties of JGI, conjugation between JGI and carboxymethyl chitosan (CTS) through ultrasound-stirring assisted Maillard reaction (UTSA-MR) was investigated and optimized.					
35868209	2	43	theme	Maillard	436:443	arg1	UTSA-MR					455:461	UTSA-MR	455:461	UTSA-MR	455:461	To enhance the stability and properties of JGI, conjugation between JGI and carboxymethyl chitosan (CTS) through ultrasound-stirring assisted Maillard reaction (UTSA-MR) was investigated and optimized.					
35868209	0	44	theme	carboxymethyl	53:65	arg1	chitosan					67:74	carboxymethyl chitosan	53:74	carboxymethyl chitosan	53:74	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	5	45	dep	CTS/JGI	899:905	arg1	w/w					913:915	w/w	913:915	w/w	913:915	Under the optimal glycation, the conjugate (CTS/JGI, 2:1, w/w; CTS-JGI-2) exhibited the best emulsifying ability and stability against NaCl solution, in vitro antioxidant activity, and cholesterol-lowering ability.					
35868209	5	45	dep	CTS/JGI	899:905	arg1	2:1					908:910	2:1	908:910	2:1	908:910	Under the optimal glycation, the conjugate (CTS/JGI, 2:1, w/w; CTS-JGI-2) exhibited the best emulsifying ability and stability against NaCl solution, in vitro antioxidant activity, and cholesterol-lowering ability.					
35868209	5	45	dep	CTS/JGI	899:905	arg1	CTS-JGI-2					918:926	CTS-JGI-2	918:926	CTS-JGI-2	918:926	Under the optimal glycation, the conjugate (CTS/JGI, 2:1, w/w; CTS-JGI-2) exhibited the best emulsifying ability and stability against NaCl solution, in vitro antioxidant activity, and cholesterol-lowering ability.					
35868209	5	46	theme	cholesterol-lowering	1040:1059	arg1	ability					1061:1067	cholesterol-lowering ability	1040:1067	cholesterol-lowering ability	1040:1067	Under the optimal glycation, the conjugate (CTS/JGI, 2:1, w/w; CTS-JGI-2) exhibited the best emulsifying ability and stability against NaCl solution, in vitro antioxidant activity, and cholesterol-lowering ability.					
35868209	1	47	contain	possess	249:255	arg1	isolate					219:225	Jiuzao glutelin isolate	203:225	Jiuzao glutelin isolate (JGI)	203:231	Jiuzao glutelin isolate (JGI) was reported to possess interface and functional properties.					
35868209	1	47	contain	possess	249:255	arg2	properties					282:291	interface and functional properties	257:291	interface and functional properties	257:291	Jiuzao glutelin isolate (JGI) was reported to possess interface and functional properties.					
35868209	1	47	contain	possess	249:255	arg1	JGI					228:230	JGI	228:230	JGI	228:230	Jiuzao glutelin isolate (JGI) was reported to possess interface and functional properties.					
35868209	3	48	theme	distribution	521:532	arg1	changes					500:506	The changes	496:506	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI	496:600	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
35868209	0	49	theme	resveratrol/quercetin	163:183	arg1	reaction					117:124	assisted Maillard reaction	99:124	assisted Maillard reaction	99:124	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	0	49	theme	resveratrol/quercetin	163:183	arg1	effect					145:150	its protective effect	130:150	its protective effect of loading resveratrol/quercetin in nano-emulsion	130:200	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	3	50	theme	structure	545:553	arg1	changes					500:506	The changes	496:506	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI	496:600	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
35868209	6	51	theme	improved	1118:1125	arg1	RES					1140:1142	RES	1140:1142	RES	1140:1142	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	6	51	theme	improved	1118:1125	arg1	resveratrol					1127:1137	nano-emulsion improved resveratrol	1104:1137	nano-emulsion improved resveratrol (RES)	1104:1143	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	6	52	theme	encapsulation	1165:1177	arg1	efficiency					1179:1188	oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency	1091:1188	oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE)	1091:1224	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	6	53	theme	simulated	1251:1259	arg1	process					1271:1277	the simulated digestion process	1247:1277	the simulated digestion process	1247:1277	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	5	54	theme	in	1005:1006	arg1	activity					1026:1033	in vitro antioxidant activity	1005:1033	in vitro antioxidant activity	1005:1033	Under the optimal glycation, the conjugate (CTS/JGI, 2:1, w/w; CTS-JGI-2) exhibited the best emulsifying ability and stability against NaCl solution, in vitro antioxidant activity, and cholesterol-lowering ability.					
35868209	5	55	theme	best	943:946	arg1	ability					960:966	emulsifying ability	948:966	emulsifying ability	948:966	Under the optimal glycation, the conjugate (CTS/JGI, 2:1, w/w; CTS-JGI-2) exhibited the best emulsifying ability and stability against NaCl solution, in vitro antioxidant activity, and cholesterol-lowering ability.					
35868209	4	56	dep	CTS-JGI	745:751	arg1	2:1					788:790	2:1	788:790	2:1	788:790	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	4	56	dep	CTS-JGI	745:751	arg1	4:1					797:799	4:1	797:799	4:1	797:799	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	4	56	dep	CTS-JGI	745:751	arg1	1:1					783:785	1:1	783:785	1:1	783:785	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	6	57	theme	nano-emulsion	1104:1116	arg1	RES					1140:1142	RES	1140:1142	RES	1140:1142	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	6	57	theme	nano-emulsion	1104:1116	arg1	resveratrol					1127:1137	nano-emulsion improved resveratrol	1104:1137	nano-emulsion improved resveratrol (RES)	1104:1143	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	4	58	dep	conjugates	733:742	arg1	CTS-JGI					745:751	CTS-JGI	745:751	CTS-JGI	745:751	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	6	59	theme	oil-in-water	1091:1102	arg1	efficiency					1179:1188	oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency	1091:1188	oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE)	1091:1224	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	0	60	from	reaction	117:124	arg1	nano-emulsion					188:200	nano-emulsion	188:200	nano-emulsion	188:200	Preparation of modified Jiuzao glutelin isolate with carboxymethyl chitosan by ultrasound-stirring assisted Maillard reaction and its protective effect of loading resveratrol/quercetin in nano-emulsion.					
35868209	4	61	theme	0.5:1	776:780	arg1	ratios					766:771	weight ratios	759:771	weight ratios of 0.5:1	759:780	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	8	62	theme	stabilized	1686:1695	arg1	nano-emulsion					1697:1709	the stabilized nano-emulsion	1682:1709	the stabilized nano-emulsion	1682:1709	CTS-JGI-2 is a potential conjugate with functions that can be used to encapsulate functional substances in the stabilized nano-emulsion.					
35868209	3	63	theme	molecular	511:519	arg1	distribution					521:532	molecular distribution	511:532	molecular distribution	511:532	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
35868209	6	64	theme	resveratrol	1127:1137	arg1	efficiency					1179:1188	oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency	1091:1188	oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE)	1091:1224	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	6	65	theme	hydrogen	1345:1352	arg1	bonds					1354:1358	hydrogen bonds	1345:1358	hydrogen bonds	1345:1358	CTS-JGI-2 stabilized oil-in-water nano-emulsion improved resveratrol (RES) and quercetin (QUE) encapsulation efficiency (80.96% for RES and 93.13% for QUE) and stability during the simulated digestion process (73.23% for RES and 77.94% for QUE) due to the connection through hydrogen bonds, pi-anion, pi-sigma, and donors between CTS-JGI and RES/QUE.					
35868209	5	66	theme	antioxidant	1014:1024	arg1	activity					1026:1033	in vitro antioxidant activity	1005:1033	in vitro antioxidant activity	1005:1033	Under the optimal glycation, the conjugate (CTS/JGI, 2:1, w/w; CTS-JGI-2) exhibited the best emulsifying ability and stability against NaCl solution, in vitro antioxidant activity, and cholesterol-lowering ability.					
35868209	5	67	dep	ability	960:966	arg1	the					939:941	the	939:941	the	939:941	Under the optimal glycation, the conjugate (CTS/JGI, 2:1, w/w; CTS-JGI-2) exhibited the best emulsifying ability and stability against NaCl solution, in vitro antioxidant activity, and cholesterol-lowering ability.					
35868209	4	68	theme	thermal	707:713	arg1	stability					715:723	thermal stability	707:723	thermal stability	707:723	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	2	69	theme	ultrasound-stirring	407:425	arg1	reaction					445:452	ultrasound-stirring assisted Maillard reaction	407:452	ultrasound-stirring assisted Maillard reaction (UTSA-MR)	407:462	To enhance the stability and properties of JGI, conjugation between JGI and carboxymethyl chitosan (CTS) through ultrasound-stirring assisted Maillard reaction (UTSA-MR) was investigated and optimized.					
35868209	2	69	theme	ultrasound-stirring	407:425	arg1	UTSA-MR					455:461	UTSA-MR	455:461	UTSA-MR	455:461	To enhance the stability and properties of JGI, conjugation between JGI and carboxymethyl chitosan (CTS) through ultrasound-stirring assisted Maillard reaction (UTSA-MR) was investigated and optimized.					
35868209	1	70	theme	interface	257:265	arg1	properties					282:291	interface and functional properties	257:291	interface and functional properties	257:291	Jiuzao glutelin isolate (JGI) was reported to possess interface and functional properties.					
35868209	4	71	dep	increased	821:829	arg1	compared					831:838	compared	831:838	compared to native JGI	831:852	The solubility, foaming property and stability, viscosity, and thermal stability of four conjugates (CTS-JGI, with weight ratios of 0.5:1, 1:1, 2:1, and 4:1) were significantly increased compared to native JGI.					
35868209	3	72	theme	morphology	556:565	arg1	changes					500:506	The changes	496:506	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI	496:600	The changes of molecular distribution, secondary structure, morphology, and amino acid composition of JGI were detected after conjugation with CTS.					
36286414	0	0	theme	Bone	109:112	arg1	Engineering					121:131	Bone Tissue Engineering	109:131	Bone Tissue Engineering	109:131	Fucoidan-Incorporated Composite Scaffold Stimulates Osteogenic Differentiation of Mesenchymal Stem Cells for Bone Tissue Engineering.					
36286414	4	1	theme	porosity	723:730	arg1	roughness					746:754	porosity and nanoscale roughness	723:754	porosity and nanoscale roughness	723:754	The SEM and AFM reveal highly suitable surface properties, such as porosity and nanoscale roughness.					
36286414	11	2	dep	graft	1554:1558	arg1	substitute					1560:1569	substitute	1560:1569	substitute for orthopedic applications	1560:1597	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.					
36286414	4	3	theme	nanoscale	736:744	arg1	roughness					746:754	porosity and nanoscale roughness	723:754	porosity and nanoscale roughness	723:754	The SEM and AFM reveal highly suitable surface properties, such as porosity and nanoscale roughness.					
36286414	11	4	theme	enriching	1459:1467	arg1	results					1490:1496	The physical, structural, and enriching osteogenic potential results	1429:1496	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F	1429:1511	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.					
36286414	11	5	theme	physical	1433:1440	arg1	results					1490:1496	The physical, structural, and enriching osteogenic potential results	1429:1496	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F	1429:1511	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.					
36286414	8	6	theme	formation	1075:1083	arg1	ability					1085:1091	The apatite formation ability	1063:1091	The apatite formation ability of Alg-HA-GO-F in the SBF solution	1063:1126	The apatite formation ability of Alg-HA-GO-F in the SBF solution predicts the scaffold's osteointegration and bone-bonding capability.					
36286414	11	7	theme	Alg-HA-GO-F	1501:1511	arg1	results					1490:1496	The physical, structural, and enriching osteogenic potential results	1429:1496	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F	1429:1511	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.					
36286414	2	8	dep	graft	509:513	arg1	substitute					515:524	substitute	515:524	substitute	515:524	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	6	9	theme	slower	863:868	arg1	degradation					870:880	the slower degradation	859:880	the slower degradation	859:880	The findings demonstrate the slower degradation and improved protein adsorption in the fucoidan-loaded scaffolds.					
36286414	2	10	dep	calcium	387:393	arg1	Alg-HA-GO-F					464:474	Alg-HA-GO-F	464:474	Alg-HA-GO-F	464:474	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	2	10	dep	calcium	387:393	arg1	oxide					457:461	crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide	395:461	calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F)	387:475	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	2	11	from	ilicifolium	320:330	arg1	fucoidan					296:303	a fucoidan	294:303	a fucoidan	294:303	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	2	11	from	ilicifolium	320:330	arg1	graft					509:513	a bone graft	502:513	a bone graft substitute	502:524	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	0	12	theme	Tissue	114:119	arg1	Engineering					121:131	Bone Tissue Engineering	109:131	Bone Tissue Engineering	109:131	Fucoidan-Incorporated Composite Scaffold Stimulates Osteogenic Differentiation of Mesenchymal Stem Cells for Bone Tissue Engineering.					
36286414	9	13	theme	greater	1253:1259	arg1	proliferation					1266:1278	a 1.5X times greater cell proliferation	1240:1278	a 1.5X times greater cell proliferation	1240:1278	In vitro studies using C3H10T1/2 revealed a 1.5X times greater cell proliferation in the fucoidan-loaded scaffold than in the control.					
36286414	11	14	theme	osteogenic	1469:1478	arg1	results					1490:1496	The physical, structural, and enriching osteogenic potential results	1429:1496	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F	1429:1511	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.					
36286414	8	15	theme	Alg-HA-GO-F	1096:1106	arg1	ability					1085:1091	The apatite formation ability	1063:1091	The apatite formation ability of Alg-HA-GO-F in the SBF solution	1063:1126	The apatite formation ability of Alg-HA-GO-F in the SBF solution predicts the scaffold's osteointegration and bone-bonding capability.					
36286414	9	16	theme	cell	1261:1264	arg1	proliferation					1266:1278	a 1.5X times greater cell proliferation	1240:1278	a 1.5X times greater cell proliferation	1240:1278	In vitro studies using C3H10T1/2 revealed a 1.5X times greater cell proliferation in the fucoidan-loaded scaffold than in the control.					
36286414	11	17	theme	structural	1443:1452	arg1	results					1490:1496	The physical, structural, and enriching osteogenic potential results	1429:1496	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F	1429:1511	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.					
36286414	2	18	theme	graphene	448:455	arg1	Alg-HA-GO-F					464:474	Alg-HA-GO-F	464:474	Alg-HA-GO-F	464:474	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	2	18	theme	graphene	448:455	arg1	oxide					457:461	crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide	395:461	calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F)	387:475	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	0	19	theme	Composite	22:30	arg1	Scaffold					32:39	Fucoidan-Incorporated Composite Scaffold	0:39	Fucoidan-Incorporated Composite Scaffold	0:39	Fucoidan-Incorporated Composite Scaffold Stimulates Osteogenic Differentiation of Mesenchymal Stem Cells for Bone Tissue Engineering.					
36286414	2	20	link	crosslinked	395:405	arg1	Alg-HA-GO-F					464:474	Alg-HA-GO-F	464:474	Alg-HA-GO-F	464:474	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	2	20	link	crosslinked	395:405	arg1	oxide					457:461	crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide	395:461	calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F)	387:475	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	5	21	theme	mechanical	794:803	arg1	strength					805:812	the mechanical strength	790:812	the mechanical strength of the Alg-HA-GO-F	790:831	The incorporation of GO enhanced the mechanical strength of the Alg-HA-GO-F.					
36286414	9	22	dep	In	1198:1199	arg1	vitro					1201:1205	vitro	1201:1205	vitro	1201:1205	In vitro studies using C3H10T1/2 revealed a 1.5X times greater cell proliferation in the fucoidan-loaded scaffold than in the control.					
36286414	2	23	theme	hydroxyapatite-nano	428:446	arg1	Alg-HA-GO-F					464:474	Alg-HA-GO-F	464:474	Alg-HA-GO-F	464:474	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	2	23	theme	hydroxyapatite-nano	428:446	arg1	oxide					457:461	crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide	395:461	calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F)	387:475	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	0	24	theme	Fucoidan-Incorporated	0:20	arg1	Scaffold					32:39	Fucoidan-Incorporated Composite Scaffold	0:39	Fucoidan-Incorporated Composite Scaffold	0:39	Fucoidan-Incorporated Composite Scaffold Stimulates Osteogenic Differentiation of Mesenchymal Stem Cells for Bone Tissue Engineering.					
36286414	8	25	theme	SBF	1115:1117	arg1	solution					1119:1126	the SBF solution	1111:1126	the SBF solution	1111:1126	The apatite formation ability of Alg-HA-GO-F in the SBF solution predicts the scaffold's osteointegration and bone-bonding capability.					
36286414	8	26	theme	apatite	1067:1073	arg1	ability					1085:1091	The apatite formation ability	1063:1091	The apatite formation ability of Alg-HA-GO-F in the SBF solution	1063:1126	The apatite formation ability of Alg-HA-GO-F in the SBF solution predicts the scaffold's osteointegration and bone-bonding capability.					
36286414	3	27	theme	structural	610:619	arg1	integration					621:631	the structural integration	606:631	the structural integration between the materials	606:653	The physiochemical characterization that includes FT-IR, XRD, and TGA confirms the structural integration between the materials.					
36286414	11	28	theme	bone	1549:1552	arg1	it					1527:1528	it	1527:1528	it	1527:1528	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.					
36286414	11	28	theme	bone	1549:1552	arg1	graft					1554:1558	a potential bone graft	1537:1558	a potential bone graft substitute for orthopedic applications	1537:1597	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.					
36286414	2	29	theme	alginate-nano	414:426	arg1	Alg-HA-GO-F					464:474	Alg-HA-GO-F	464:474	Alg-HA-GO-F	464:474	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	2	29	theme	alginate-nano	414:426	arg1	oxide					457:461	crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide	395:461	calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F)	387:475	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	9	30	theme	fucoidan-loaded	1287:1301	arg1	scaffold					1303:1310	the fucoidan-loaded scaffold	1283:1310	the fucoidan-loaded scaffold	1283:1310	In vitro studies using C3H10T1/2 revealed a 1.5X times greater cell proliferation in the fucoidan-loaded scaffold than in the control.					
36286414	10	31	theme	phosphatase	1388:1398	arg1	activity					1419:1426	the augmented alkaline phosphatase and mineralization activity	1365:1426	the augmented alkaline phosphatase and mineralization activity	1365:1426	Further, the results determined the augmented alkaline phosphatase and mineralization activity.					
36286414	7	32	from	release	971:977	arg1	PBS					994:996	PBS	994:996	PBS for 120 h	994:1006	The slow and sustained release of fucoidan in PBS for 120 h provides the developed system with an added advantage.					
36286414	8	33	from	ability	1085:1091	arg1	solution					1119:1126	the SBF solution	1111:1126	the SBF solution	1111:1126	The apatite formation ability of Alg-HA-GO-F in the SBF solution predicts the scaffold's osteointegration and bone-bonding capability.					
36286414	11	34	theme	potential	1539:1547	arg1	it					1527:1528	it	1527:1528	it	1527:1528	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.					
36286414	11	34	theme	potential	1539:1547	arg1	graft					1554:1558	a potential bone graft	1537:1558	a potential bone graft substitute for orthopedic applications	1537:1597	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.					
36286414	10	35	theme	alkaline	1379:1386	arg1	phosphatase					1388:1398	alkaline phosphatase	1379:1398	alkaline phosphatase	1379:1398	Further, the results determined the augmented alkaline phosphatase and mineralization activity.					
36286414	1	36	theme	graft	161:165	arg1	procedures					167:176	bone graft procedures	156:176	bone graft procedures	156:176	Globally, millions of bone graft procedures are being performed by clinicians annually to treat the rising prevalence of bone defects.					
36286414	5	37	theme	Alg-HA-GO-F	821:831	arg1	strength					805:812	the mechanical strength	790:812	the mechanical strength of the Alg-HA-GO-F	790:831	The incorporation of GO enhanced the mechanical strength of the Alg-HA-GO-F.					
36286414	3	38	theme	physiochemical	531:544	arg1	characterization					546:561	The physiochemical characterization	527:561	The physiochemical characterization that includes FT-IR, XRD, and TGA	527:595	The physiochemical characterization that includes FT-IR, XRD, and TGA confirms the structural integration between the materials.					
36286414	6	39	theme	fucoidan-loaded	921:935	arg1	scaffolds					937:945	the fucoidan-loaded scaffolds	917:945	the fucoidan-loaded scaffolds	917:945	The findings demonstrate the slower degradation and improved protein adsorption in the fucoidan-loaded scaffolds.					
36286414	10	40	theme	augmented	1369:1377	arg1	activity					1419:1426	the augmented alkaline phosphatase and mineralization activity	1365:1426	the augmented alkaline phosphatase and mineralization activity	1365:1426	Further, the results determined the augmented alkaline phosphatase and mineralization activity.					
36286414	4	41	theme	suitable	686:693	arg1	properties					703:712	highly suitable surface properties	679:712	highly suitable surface properties	679:712	The SEM and AFM reveal highly suitable surface properties, such as porosity and nanoscale roughness.					
36286414	2	42	theme	bone	504:507	arg1	fucoidan					296:303	a fucoidan	294:303	a fucoidan	294:303	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	2	42	theme	bone	504:507	arg1	graft					509:513	a bone graft	502:513	a bone graft substitute	502:524	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	0	43	theme	Osteogenic	52:61	arg1	Differentiation					63:77	Osteogenic Differentiation	52:77	Osteogenic Differentiation of Mesenchymal Stem Cells for Bone Tissue Engineering	52:131	Fucoidan-Incorporated Composite Scaffold Stimulates Osteogenic Differentiation of Mesenchymal Stem Cells for Bone Tissue Engineering.					
36286414	5	44	theme	GO	778:779	arg1	incorporation					761:773	The incorporation	757:773	The incorporation of GO	757:779	The incorporation of GO enhanced the mechanical strength of the Alg-HA-GO-F.					
36286414	1	45	theme	rising	234:239	arg1	prevalence					241:250	the rising prevalence	230:250	the rising prevalence of bone defects	230:266	Globally, millions of bone graft procedures are being performed by clinicians annually to treat the rising prevalence of bone defects.					
36286414	0	46	theme	Mesenchymal	82:92	arg1	Cells					99:103	Mesenchymal Stem Cells	82:103	Mesenchymal Stem Cells	82:103	Fucoidan-Incorporated Composite Scaffold Stimulates Osteogenic Differentiation of Mesenchymal Stem Cells for Bone Tissue Engineering.					
36286414	11	47	theme	potential	1480:1488	arg1	results					1490:1496	The physical, structural, and enriching osteogenic potential results	1429:1496	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F	1429:1511	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.					
36286414	9	48	theme	In	1198:1199	arg1	studies					1207:1213	In vitro studies	1198:1213	In vitro studies using C3H10T1/2	1198:1229	In vitro studies using C3H10T1/2 revealed a 1.5X times greater cell proliferation in the fucoidan-loaded scaffold than in the control.					
36286414	6	49	theme	protein	895:901	arg1	adsorption					903:912	improved protein adsorption	886:912	improved protein adsorption	886:912	The findings demonstrate the slower degradation and improved protein adsorption in the fucoidan-loaded scaffolds.					
36286414	1	50	theme	procedures	167:176	arg1	millions					144:151	millions	144:151	millions of bone graft procedures	144:176	Globally, millions of bone graft procedures are being performed by clinicians annually to treat the rising prevalence of bone defects.					
36286414	2	51	theme	osteo-inductive	351:365	arg1	scaffold					367:374	an osteo-inductive scaffold	348:374	an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F)	348:475	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	11	52	theme	orthopedic	1575:1584	arg1	applications					1586:1597	orthopedic applications	1575:1597	orthopedic applications	1575:1597	The physical, structural, and enriching osteogenic potential results of Alg-HA-GO-F indicate that it can be a potential bone graft substitute for orthopedic applications.					
36286414	4	53	dep	SEM	660:662	arg1	The					656:658	The	656:658	The	656:658	The SEM and AFM reveal highly suitable surface properties, such as porosity and nanoscale roughness.					
36286414	7	54	theme	developed	1021:1029	arg1	system					1031:1036	the developed system	1017:1036	the developed system with an added advantage	1017:1060	The slow and sustained release of fucoidan in PBS for 120 h provides the developed system with an added advantage.					
36286414	7	55	theme	fucoidan	982:989	arg1	release					971:977	The slow and sustained release	948:977	The slow and sustained release of fucoidan in PBS for 120 h	948:1006	The slow and sustained release of fucoidan in PBS for 120 h provides the developed system with an added advantage.					
36286414	7	56	with	system	1031:1036	arg1	advantage					1052:1060	an added advantage	1043:1060	an added advantage	1043:1060	The slow and sustained release of fucoidan in PBS for 120 h provides the developed system with an added advantage.					
36286414	8	57	theme	bone-bonding	1173:1184	arg1	capability					1186:1195	bone-bonding capability	1173:1195	bone-bonding capability	1173:1195	The apatite formation ability of Alg-HA-GO-F in the SBF solution predicts the scaffold's osteointegration and bone-bonding capability.					
36286414	6	58	theme	improved	886:893	arg1	adsorption					903:912	improved protein adsorption	886:912	improved protein adsorption	886:912	The findings demonstrate the slower degradation and improved protein adsorption in the fucoidan-loaded scaffolds.					
36286414	1	59	theme	bone	156:159	arg1	procedures					167:176	bone graft procedures	156:176	bone graft procedures	156:176	Globally, millions of bone graft procedures are being performed by clinicians annually to treat the rising prevalence of bone defects.					
36286414	7	60	theme	sustained	961:969	arg1	release					971:977	The slow and sustained release	948:977	The slow and sustained release of fucoidan in PBS for 120 h	948:1006	The slow and sustained release of fucoidan in PBS for 120 h provides the developed system with an added advantage.					
36286414	10	61	theme	mineralization	1404:1417	arg1	activity					1419:1426	the augmented alkaline phosphatase and mineralization activity	1365:1426	the augmented alkaline phosphatase and mineralization activity	1365:1426	Further, the results determined the augmented alkaline phosphatase and mineralization activity.					
36286414	0	62	theme	Cells	99:103	arg1	Differentiation					63:77	Osteogenic Differentiation	52:77	Osteogenic Differentiation of Mesenchymal Stem Cells for Bone Tissue Engineering	52:131	Fucoidan-Incorporated Composite Scaffold Stimulates Osteogenic Differentiation of Mesenchymal Stem Cells for Bone Tissue Engineering.					
36286414	7	63	theme	added	1046:1050	arg1	advantage					1052:1060	an added advantage	1043:1060	an added advantage	1043:1060	The slow and sustained release of fucoidan in PBS for 120 h provides the developed system with an added advantage.					
36286414	2	64	theme	sodium	407:412	arg1	Alg-HA-GO-F					464:474	Alg-HA-GO-F	464:474	Alg-HA-GO-F	464:474	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	2	64	theme	sodium	407:412	arg1	oxide					457:461	crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide	395:461	calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F)	387:475	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	4	65	theme	surface	695:701	arg1	properties					703:712	highly suitable surface properties	679:712	highly suitable surface properties	679:712	The SEM and AFM reveal highly suitable surface properties, such as porosity and nanoscale roughness.					
36286414	1	66	theme	bone	255:258	arg1	defects					260:266	bone defects	255:266	bone defects	255:266	Globally, millions of bone graft procedures are being performed by clinicians annually to treat the rising prevalence of bone defects.					
36286414	0	67	theme	Stem	94:97	arg1	Cells					99:103	Mesenchymal Stem Cells	82:103	Mesenchymal Stem Cells	82:103	Fucoidan-Incorporated Composite Scaffold Stimulates Osteogenic Differentiation of Mesenchymal Stem Cells for Bone Tissue Engineering.					
36286414	2	68	theme	crosslinked	395:405	arg1	Alg-HA-GO-F					464:474	Alg-HA-GO-F	464:474	Alg-HA-GO-F	464:474	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	2	68	theme	crosslinked	395:405	arg1	oxide					457:461	crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide	395:461	calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F)	387:475	Here, the study designed a fucoidan from Sargassum ilicifolium incorporated in an osteo-inductive scaffold comprising calcium crosslinked sodium alginate-nano hydroxyapatite-nano graphene oxide (Alg-HA-GO-F), which tends to serve as a bone graft substitute.					
36286414	7	69	theme	slow	952:955	arg1	release					971:977	The slow and sustained release	948:977	The slow and sustained release of fucoidan in PBS for 120 h	948:1006	The slow and sustained release of fucoidan in PBS for 120 h provides the developed system with an added advantage.					
36286414	1	70	theme	defects	260:266	arg1	prevalence					241:250	the rising prevalence	230:250	the rising prevalence of bone defects	230:266	Globally, millions of bone graft procedures are being performed by clinicians annually to treat the rising prevalence of bone defects.					
37028876	0	0	theme	composite	89:97	arg1	hydrogel					99:106	bacterial cellulose composite hydrogel	69:106	bacterial cellulose composite hydrogel	69:106	Growth of spiral ganglion neurons induced by graphene oxide/oxidized bacterial cellulose composite hydrogel.					
37028876	3	1	dep	property	701:708	arg1	met					747:749	met	747:749	met	747:749	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	3	1	dep	property	701:708	arg1	the					672:674	the	672:674	the	672:674	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	2	2	theme	hydrogel	331:338	arg1	form					313:316	a new form	307:316	a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel)	307:442	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	3	3	theme	hybrid	808:813	arg1	matrix					815:820	the GO/TOBC hybrid matrix	796:820	the GO/TOBC hybrid matrix	796:820	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	1	4	theme	central	238:244	arg1	system					255:260	the central auditory system	234:260	the central auditory system	234:260	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	0	5	theme	cellulose	79:87	arg1	hydrogel					99:106	bacterial cellulose composite hydrogel	69:106	bacterial cellulose composite hydrogel	69:106	Growth of spiral ganglion neurons induced by graphene oxide/oxidized bacterial cellulose composite hydrogel.					
37028876	3	6	theme	controllable	676:687	arg1	property					701:708	controllable hydrophilic property	676:708	controllable hydrophilic property	676:708	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	2	7	theme	bioactive	321:329	arg1	hydrogel					331:338	bioactive hydrogel	321:338	bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel)	321:442	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	3	8	theme	SGNs	873:876	arg1	growth					863:868	the growth	859:868	the growth of SGNs	859:876	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	1	9	theme	auditory	246:253	arg1	system					255:260	the central auditory system	234:260	the central auditory system	234:260	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	3	10	theme	hydrophilic	689:699	arg1	property					701:708	controllable hydrophilic property	676:708	controllable hydrophilic property	676:708	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	4	11	theme	quantitative	883:894	arg1	result					910:915	The quantitative real-time PCR result	879:915	The quantitative real-time PCR result	879:915	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	4	12	theme	diap3	1073:1077	arg1	levels					1063:1068	the mRNA expression levels	1043:1068	the mRNA expression levels of diap3, fscn2, and integrin β1	1043:1101	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	4	13	theme	growth	1001:1006	arg1	cones					1008:1012	growth cones	1001:1012	growth cones	1001:1012	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	2	14	theme	neurite	506:512	arg1	outgrowth					514:522	SGN neurite outgrowth	502:522	SGN neurite outgrowth	502:522	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	3	15	theme	network	532:538	arg1	structure					540:548	the network structure	528:548	the network structure of lamellar interspersed fiber	528:579	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	3	16	theme	great	832:836	arg1	potential					838:846	great potential	832:846	great potential	832:846	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	2	17	theme	SGN	502:504	arg1	outgrowth					514:522	SGN neurite outgrowth	502:522	SGN neurite outgrowth	502:522	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	2	18	theme	TEMPO-oxidized	390:403	arg1	cellulose					415:423	TEMPO-oxidized bacterial cellulose	390:423	TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel)	390:442	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	2	18	theme	TEMPO-oxidized	390:403	arg1	hydrogel					434:441	GO/TOBC hydrogel	426:441	GO/TOBC hydrogel	426:441	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	3	19	dep	hydrogels	605:613	arg1	simulated					620:628	simulated	620:628	simulated	620:628	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	3	20	theme	appropriate	714:724	arg1	modulus					734:740	appropriate Young's modulus	714:740	appropriate Young's modulus	714:740	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	3	21	theme	Young	726:730	arg1	modulus					734:740	appropriate Young's modulus	714:740	appropriate Young's modulus	714:740	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	3	22	theme	lamellar	553:560	arg1	fiber					575:579	lamellar interspersed fiber	553:579	lamellar interspersed fiber	553:579	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	2	23	theme	GO/TOBC	426:432	arg1	cellulose					415:423	TEMPO-oxidized bacterial cellulose	390:423	TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel)	390:442	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	2	23	theme	GO/TOBC	426:432	arg1	hydrogel					434:441	GO/TOBC hydrogel	426:441	GO/TOBC hydrogel	426:441	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	3	24	theme	interspersed	562:573	arg1	fiber					575:579	lamellar interspersed fiber	553:579	lamellar interspersed fiber	553:579	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	2	25	theme	graphene	366:373	arg1	oxide					375:379	topological graphene oxide	354:379	topological graphene oxide (GO)	354:384	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	0	26	theme	ganglion	17:24	arg1	neurons					26:32	spiral ganglion neurons	10:32	spiral ganglion neurons induced by graphene	10:52	Growth of spiral ganglion neurons induced by graphene oxide/oxidized bacterial cellulose composite hydrogel.					
37028876	4	27	theme	β1	1100:1101	arg1	levels					1063:1068	the mRNA expression levels	1043:1068	the mRNA expression levels of diap3, fscn2, and integrin β1	1043:1101	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	1	28	theme	auditory	185:192	arg1	transduction					202:213	the auditory signals transduction	181:213	the auditory signals transduction from hair cells to the central auditory system	181:260	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	0	29	theme	spiral	10:15	arg1	neurons					26:32	spiral ganglion neurons	10:32	spiral ganglion neurons induced by graphene	10:52	Growth of spiral ganglion neurons induced by graphene oxide/oxidized bacterial cellulose composite hydrogel.					
37028876	1	30	theme	significant	273:283	arg1	loss					293:296	significant hearing loss	273:296	significant hearing loss	273:296	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	2	31	theme	topological	354:364	arg1	oxide					375:379	topological graphene oxide	354:379	topological graphene oxide (GO)	354:384	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	1	32	theme	signals	194:200	arg1	transduction					202:213	the auditory signals transduction	181:213	the auditory signals transduction from hair cells to the central auditory system	181:260	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	4	33	theme	filopodia	1018:1026	arg1	development					986:996	the development	982:996	the development of growth cones and filopodia	982:1026	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	1	34	theme	hearing	285:291	arg1	loss					293:296	significant hearing loss	273:296	significant hearing loss	273:296	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	4	35	theme	expression	1052:1061	arg1	levels					1063:1068	the mRNA expression levels	1043:1068	the mRNA expression levels of diap3, fscn2, and integrin β1	1043:1101	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	5	36	theme	biomimetic	1201:1210	arg1	grafts					1218:1223	biomimetic nerve grafts	1201:1223	biomimetic nerve grafts for repairing or replacing nerve defects	1201:1264	These results suggest that GO/TOBC hydrogel scaffolds have the potential to be used to construct biomimetic nerve grafts for repairing or replacing nerve defects.					
37028876	0	37	theme	neurons	26:32	arg1	Growth					0:5	Growth	0:5	Growth of spiral ganglion neurons induced by graphene	0:52	Growth of spiral ganglion neurons induced by graphene oxide/oxidized bacterial cellulose composite hydrogel.					
37028876	3	38	theme	microenvironment	778:793	arg1	requirements					757:768	those requirements	751:768	those requirements of SGNs microenvironment	751:793	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	4	39	theme	GO/TOBC	936:942	arg1	hydrogel					944:951	the GO/TOBC hydrogel	932:951	the GO/TOBC hydrogel	932:951	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	3	40	theme	GO/TOBC	597:603	arg1	hydrogels					605:613	GO/TOBC hydrogels	597:613	GO/TOBC hydrogels well simulated the structure and morphology of ECM	597:664	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	4	41	theme	PCR	906:908	arg1	result					910:915	The quantitative real-time PCR result	879:915	The quantitative real-time PCR result	879:915	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	3	42	theme	fiber	575:579	arg1	structure					540:548	the network structure	528:548	the network structure of lamellar interspersed fiber	528:579	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	5	43	theme	GO/TOBC	1131:1137	arg1	scaffolds					1148:1156	GO/TOBC hydrogel scaffolds	1131:1156	GO/TOBC hydrogel scaffolds	1131:1156	These results suggest that GO/TOBC hydrogel scaffolds have the potential to be used to construct biomimetic nerve grafts for repairing or replacing nerve defects.					
37028876	5	44	theme	hydrogel	1139:1146	arg1	scaffolds					1148:1156	GO/TOBC hydrogel scaffolds	1131:1156	GO/TOBC hydrogel scaffolds	1131:1156	These results suggest that GO/TOBC hydrogel scaffolds have the potential to be used to construct biomimetic nerve grafts for repairing or replacing nerve defects.					
37028876	4	45	theme	real-time	896:904	arg1	result					910:915	The quantitative real-time PCR result	879:915	The quantitative real-time PCR result	879:915	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	1	46	theme	neurons	155:161	arg1	degeneration					123:134	degeneration	123:134	degeneration	123:134	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	1	46	theme	neurons	155:161	arg1	damage					113:118	damage	113:118	damage	113:118	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	3	47	theme	SGNs	773:776	arg1	microenvironment					778:793	SGNs microenvironment	773:793	SGNs microenvironment	773:793	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	5	48	contain	have	1158:1161	arg1	scaffolds					1148:1156	GO/TOBC hydrogel scaffolds	1131:1156	GO/TOBC hydrogel scaffolds	1131:1156	These results suggest that GO/TOBC hydrogel scaffolds have the potential to be used to construct biomimetic nerve grafts for repairing or replacing nerve defects.					
37028876	5	48	contain	have	1158:1161	arg2	potential					1167:1175	the potential to be used to construct biomimetic nerve grafts for repairing or replacing nerve defects	1163:1264	the potential to be used to construct biomimetic nerve grafts for repairing or replacing nerve defects	1163:1264	These results suggest that GO/TOBC hydrogel scaffolds have the potential to be used to construct biomimetic nerve grafts for repairing or replacing nerve defects.					
37028876	2	49	dep	oxide	375:379	arg1	GO					382:383	GO	382:383	GO	382:383	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	2	50	theme	new	309:311	arg1	form					313:316	a new form	307:316	a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel)	307:442	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	3	51	dep	structure	634:642	arg1	the					630:632	the	630:632	the	630:632	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	1	52	theme	hair	220:223	arg1	cells					225:229	hair cells	220:229	hair cells	220:229	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	1	53	from	cells	225:229	arg1	transduction					202:213	the auditory signals transduction	181:213	the auditory signals transduction from hair cells to the central auditory system	181:260	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	3	54	theme	ECM	662:664	arg1	morphology					648:657	morphology	648:657	morphology	648:657	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	3	54	theme	ECM	662:664	arg1	structure					634:642	structure	634:642	structure	634:642	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
37028876	1	55	theme	spiral	139:144	arg1	neurons					155:161	spiral ganglion neurons	139:161	spiral ganglion neurons (SGNs)	139:168	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	1	55	theme	spiral	139:144	arg1	SGNs					164:167	SGNs	164:167	SGNs	164:167	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	4	56	theme	integrin	1091:1098	arg1	β1					1100:1101	integrin β1	1091:1101	integrin β1	1091:1101	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	0	57	theme	bacterial	69:77	arg1	hydrogel					99:106	bacterial cellulose composite hydrogel	69:106	bacterial cellulose composite hydrogel	69:106	Growth of spiral ganglion neurons induced by graphene oxide/oxidized bacterial cellulose composite hydrogel.					
37028876	2	58	theme	bacterial	405:413	arg1	cellulose					415:423	TEMPO-oxidized bacterial cellulose	390:423	TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel)	390:442	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	2	58	theme	bacterial	405:413	arg1	hydrogel					434:441	GO/TOBC hydrogel	426:441	GO/TOBC hydrogel	426:441	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	1	59	theme	ganglion	146:153	arg1	neurons					155:161	spiral ganglion neurons	139:161	spiral ganglion neurons (SGNs)	139:168	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	1	59	theme	ganglion	146:153	arg1	SGNs					164:167	SGNs	164:167	SGNs	164:167	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	4	60	theme	fscn2	1080:1084	arg1	levels					1063:1068	the mRNA expression levels	1043:1068	the mRNA expression levels of diap3, fscn2, and integrin β1	1043:1101	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	2	61	theme	favorable	471:479	arg1	microenvironment					481:496	a favorable microenvironment	469:496	a favorable microenvironment for SGN neurite outgrowth	469:522	Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth.					
37028876	4	62	theme	cones	1008:1012	arg1	development					986:996	the development	982:996	the development of growth cones and filopodia	982:1026	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	4	63	theme	mRNA	1047:1050	arg1	levels					1063:1068	the mRNA expression levels	1043:1068	the mRNA expression levels of diap3, fscn2, and integrin β1	1043:1101	The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1.					
37028876	5	64	theme	nerve	1212:1216	arg1	grafts					1218:1223	biomimetic nerve grafts	1201:1223	biomimetic nerve grafts for repairing or replacing nerve defects	1201:1264	These results suggest that GO/TOBC hydrogel scaffolds have the potential to be used to construct biomimetic nerve grafts for repairing or replacing nerve defects.					
37028876	1	65	dep	damage	113:118	arg1	The					109:111	The	109:111	The	109:111	The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss.					
37028876	5	66	theme	nerve	1252:1256	arg1	defects					1258:1264	nerve defects	1252:1264	nerve defects	1252:1264	These results suggest that GO/TOBC hydrogel scaffolds have the potential to be used to construct biomimetic nerve grafts for repairing or replacing nerve defects.					
37028876	3	67	theme	GO/TOBC	800:806	arg1	matrix					815:820	the GO/TOBC hybrid matrix	796:820	the GO/TOBC hybrid matrix	796:820	As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs.					
36063891	3	0	theme	three-dimensional	688:704	arg1	network					719:725	a three-dimensional cross-linked network	686:725	a three-dimensional cross-linked network	686:725	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	4	1	theme	designed	828:835	arg1	material					845:852	the designed coating material	824:852	the designed coating material	824:852	Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine.					
36063891	3	2	dep	turn	646:649	arg1	integrated					651:660	integrated	651:660	integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent	651:815	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	0	3	theme	slow-release	96:107	arg1	fertilizers					109:119	slow-release fertilizers	96:119	slow-release fertilizers	96:119	Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.					
36063891	8	4	theme	water	1456:1460	arg1	capacity					1472:1479	the soil water retention capacity	1447:1479	the soil water retention capacity	1447:1479	Furthermore, the coating film enhanced the fertilizers mechanical resistance and boosted the soil water retention capacity.					
36063891	1	5	theme	adverse	182:188	arg1	effects					190:196	adverse effects	182:196	adverse effects	182:196	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	3	6	theme	cross-linking	797:809	arg1	agent					811:815	a cross-linking agent	795:815	a cross-linking agent	795:815	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	4	7	theme	Triple	903:908	arg1	Superphosphate					910:923	Triple Superphosphate	903:923	Triple Superphosphate (TSP)	903:929	Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine.					
36063891	4	7	theme	Triple	903:908	arg1	TSP					926:928	TSP	926:928	TSP	926:928	Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine.					
36063891	9	8	theme	chlorophyll	1583:1593	arg1	content					1595:1601	chlorophyll content	1583:1601	chlorophyll content	1583:1601	The agronomic evaluation has also confirmed their remarkable potential in enhancing wheat leaf area, chlorophyll content and biomass, in addition to the roots architecture and the final fruiting efficiency.					
36063891	6	9	theme	soil	1154:1157	arg1	environments					1159:1170	soil environments	1154:1170	soil environments	1154:1170	Nutrients release experiments in water as well as in soil environments have proved the effectiveness of the MC and MC/LGe coating layers in delaying the nutrients discharge.					
36063891	9	10	theme	leaf	1572:1575	arg1	area					1577:1580	enhancing wheat leaf area	1556:1580	enhancing wheat leaf area	1556:1580	The agronomic evaluation has also confirmed their remarkable potential in enhancing wheat leaf area, chlorophyll content and biomass, in addition to the roots architecture and the final fruiting efficiency.					
36063891	4	11	theme	water-soluble	931:943	arg1	fertilizers					945:955	Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers	871:955	Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine	871:981	Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine.					
36063891	10	12	theme	efficient	1757:1765	arg1	material					1775:1782	an efficient coating material	1754:1782	an efficient coating material to produce slow-release fertilizers with multifunctional performances	1754:1852	These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.					
36063891	10	12	theme	efficient	1757:1765	arg1	composite					1727:1735	this hybrid composite	1715:1735	this hybrid composite	1715:1735	These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.					
36063891	4	13	theme	coating	837:843	arg1	material					845:852	the designed coating material	824:852	the designed coating material	824:852	Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine.					
36063891	3	14	link	cross-linked	706:717	arg1	network					719:725	a three-dimensional cross-linked network	686:725	a three-dimensional cross-linked network	686:725	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	1	15	theme	higher	299:304	arg1	costs					318:322	higher operational costs	299:322	higher operational costs	299:322	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	0	16	theme	nutrients	132:140	arg1	management					142:151	nutrients management	132:151	nutrients management	132:151	Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.					
36063891	5	17	theme	coating	1055:1061	arg1	material					1063:1070	the coating material	1051:1070	the coating material	1051:1070	Chemical, physical, and biodegradation studies have confirmed that the coating material is environmentally-friendly.					
36063891	5	17	theme	coating	1055:1061	arg1	environmentally-friendly					1075:1098	environmentally-friendly	1075:1098	environmentally-friendly	1075:1098	Chemical, physical, and biodegradation studies have confirmed that the coating material is environmentally-friendly.					
36063891	6	18	theme	layers	1231:1236	arg1	effectiveness					1188:1200	the effectiveness	1184:1200	the effectiveness of the MC and MC/LGe coating layers	1184:1236	Nutrients release experiments in water as well as in soil environments have proved the effectiveness of the MC and MC/LGe coating layers in delaying the nutrients discharge.					
36063891	9	19	theme	remarkable	1532:1541	arg1	potential					1543:1551	their remarkable potential	1526:1551	their remarkable potential	1526:1551	The agronomic evaluation has also confirmed their remarkable potential in enhancing wheat leaf area, chlorophyll content and biomass, in addition to the roots architecture and the final fruiting efficiency.					
36063891	7	20	theme	coated	1311:1316	arg1	DAP					1318:1320	coated DAP	1311:1320	coated DAP	1311:1320	Besides, the nutrients release from coated DAP and TSP lasted longer than 30 days.					
36063891	10	21	used	used	1746:1749	arg2	material					1775:1782	an efficient coating material	1754:1782	an efficient coating material to produce slow-release fertilizers with multifunctional performances	1754:1852	These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.					
36063891	10	21	used	used	1746:1749	arg2	composite					1727:1735	this hybrid composite	1715:1735	this hybrid composite	1715:1735	These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.					
36063891	4	22	theme	Superphosphate	910:923	arg1	fertilizers					945:955	Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers	871:955	Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine	871:981	Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine.					
36063891	3	23	theme	cross-linked	706:717	arg1	network					719:725	a three-dimensional cross-linked network	686:725	a three-dimensional cross-linked network	686:725	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	6	24	theme	coating	1223:1229	arg1	layers					1231:1236	the MC and MC/LGe coating layers	1205:1236	layers	1231:1236	Nutrients release experiments in water as well as in soil environments have proved the effectiveness of the MC and MC/LGe coating layers in delaying the nutrients discharge.					
36063891	0	25	theme	Methylcellulose/lignin	0:21	arg1	biocomposite					23:34	Methylcellulose/lignin biocomposite	0:34	Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.	0:169	Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.					
36063891	2	26	theme	coating	468:474	arg1	material					476:483	a sustainable coating material	454:483	a sustainable coating material based on biodegradable polymers	454:515	The present work aims to design a sustainable coating material based on biodegradable polymers.					
36063891	3	27	theme	network	719:725	arg1	development					671:681	the development	667:681	the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA)	667:790	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	6	28	theme	MC/LGe	1216:1221	arg1	layers					1231:1236	the MC and MC/LGe coating layers	1205:1236	layers	1231:1236	Nutrients release experiments in water as well as in soil environments have proved the effectiveness of the MC and MC/LGe coating layers in delaying the nutrients discharge.					
36063891	3	29	theme	sustainable	578:588	arg1	plant					536:540	Alfa plant	531:540	Alfa plant	531:540	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	3	29	theme	sustainable	578:588	arg1	source					590:595	a viable sustainable source	569:595	a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent	569:815	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	7	30	from	TSP	1326:1328	arg1	release					1298:1304	the nutrients release	1284:1304	the nutrients release from coated DAP and TSP	1284:1328	Besides, the nutrients release from coated DAP and TSP lasted longer than 30 days.					
36063891	2	31	theme	sustainable	456:466	arg1	material					476:483	a sustainable coating material	454:483	a sustainable coating material based on biodegradable polymers	454:515	The present work aims to design a sustainable coating material based on biodegradable polymers.					
36063891	1	32	theme	operational	306:316	arg1	costs					318:322	higher operational costs	299:322	higher operational costs	299:322	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	5	33	theme	biodegradation	1008:1021	arg1	studies					1023:1029	Chemical, physical, and biodegradation studies	984:1029	Chemical, physical, and biodegradation studies	984:1029	Chemical, physical, and biodegradation studies have confirmed that the coating material is environmentally-friendly.					
36063891	9	34	theme	enhancing	1556:1564	arg1	area					1577:1580	enhancing wheat leaf area	1556:1580	enhancing wheat leaf area	1556:1580	The agronomic evaluation has also confirmed their remarkable potential in enhancing wheat leaf area, chlorophyll content and biomass, in addition to the roots architecture and the final fruiting efficiency.					
36063891	7	35	theme	nutrients	1288:1296	arg1	release					1298:1304	the nutrients release	1284:1304	the nutrients release from coated DAP and TSP	1284:1328	Besides, the nutrients release from coated DAP and TSP lasted longer than 30 days.					
36063891	3	36	theme	lignin	619:624	arg1	extraction					605:614	the extraction	601:614	the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent	601:815	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	9	37	theme	fruiting	1668:1675	arg1	efficiency					1677:1686	the final fruiting efficiency	1658:1686	the final fruiting efficiency	1658:1686	The agronomic evaluation has also confirmed their remarkable potential in enhancing wheat leaf area, chlorophyll content and biomass, in addition to the roots architecture and the final fruiting efficiency.					
36063891	9	38	theme	wheat	1566:1570	arg1	area					1577:1580	enhancing wheat leaf area	1556:1580	enhancing wheat leaf area	1556:1580	The agronomic evaluation has also confirmed their remarkable potential in enhancing wheat leaf area, chlorophyll content and biomass, in addition to the roots architecture and the final fruiting efficiency.					
36063891	6	39	theme	MC	1209:1210	arg1	effectiveness					1188:1200	the effectiveness	1184:1200	the effectiveness of the MC and MC/LGe coating layers	1184:1236	Nutrients release experiments in water as well as in soil environments have proved the effectiveness of the MC and MC/LGe coating layers in delaying the nutrients discharge.					
36063891	10	40	dep	material	1775:1782	arg1	produce					1787:1793	produce	1787:1793	to produce slow-release fertilizers with multifunctional performances	1784:1852	These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.					
36063891	4	41	theme	Phosphate	883:891	arg1	fertilizers					945:955	Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers	871:955	Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine	871:981	Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine.					
36063891	1	42	theme	research	404:411	arg1	circles					413:419	the research circles	400:419	the research circles	400:419	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	3	43	theme	viable	571:576	arg1	plant					536:540	Alfa plant	531:540	Alfa plant	531:540	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	3	43	theme	viable	571:576	arg1	source					590:595	a viable sustainable source	569:595	a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent	569:815	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	1	44	theme	certain	231:237	arg1	SRFs					279:282	SRFs	279:282	SRFs	279:282	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	1	44	theme	certain	231:237	arg1	fertilizers					266:276	certain polymer-based slow-release fertilizers	231:276	certain polymer-based slow-release fertilizers (SRFs)	231:283	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	2	45	theme	biodegradable	494:506	arg1	polymers					508:515	biodegradable polymers	494:515	biodegradable polymers	494:515	The present work aims to design a sustainable coating material based on biodegradable polymers.					
36063891	7	46	from	DAP	1318:1320	arg1	release					1298:1304	the nutrients release	1284:1304	the nutrients release from coated DAP and TSP	1284:1328	Besides, the nutrients release from coated DAP and TSP lasted longer than 30 days.					
36063891	9	47	theme	final	1662:1666	arg1	efficiency					1677:1686	the final fruiting efficiency	1658:1686	the final fruiting efficiency	1658:1686	The agronomic evaluation has also confirmed their remarkable potential in enhancing wheat leaf area, chlorophyll content and biomass, in addition to the roots architecture and the final fruiting efficiency.					
36063891	8	48	theme	soil	1451:1454	arg1	capacity					1472:1479	the soil water retention capacity	1447:1479	the soil water retention capacity	1447:1479	Furthermore, the coating film enhanced the fertilizers mechanical resistance and boosted the soil water retention capacity.					
36063891	6	49	theme	nutrients	1254:1262	arg1	discharge					1264:1272	the nutrients discharge	1250:1272	the nutrients discharge	1250:1272	Nutrients release experiments in water as well as in soil environments have proved the effectiveness of the MC and MC/LGe coating layers in delaying the nutrients discharge.					
36063891	1	50	theme	polymer-based	239:251	arg1	SRFs					279:282	SRFs	279:282	SRFs	279:282	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	1	50	theme	polymer-based	239:251	arg1	fertilizers					266:276	certain polymer-based slow-release fertilizers	231:276	certain polymer-based slow-release fertilizers (SRFs)	231:283	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	0	51	theme	eco-friendly	42:53	arg1	material					83:90	an eco-friendly and multifunctional coating material	39:90	an eco-friendly and multifunctional coating material for slow-release fertilizers	39:119	Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.					
36063891	4	52	theme	rotating	962:969	arg1	machine					975:981	a rotating pan machine	960:981	a rotating pan machine	960:981	Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine.					
36063891	0	53	theme	wheat	157:161	arg1	growth					163:168	wheat growth	157:168	wheat growth	157:168	Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.					
36063891	8	54	theme	mechanical	1413:1422	arg1	resistance					1424:1433	the fertilizers mechanical resistance	1397:1433	the fertilizers mechanical resistance	1397:1433	Furthermore, the coating film enhanced the fertilizers mechanical resistance and boosted the soil water retention capacity.					
36063891	1	55	theme	slow-release	253:264	arg1	SRFs					279:282	SRFs	279:282	SRFs	279:282	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	1	55	theme	slow-release	253:264	arg1	fertilizers					266:276	certain polymer-based slow-release fertilizers	231:276	certain polymer-based slow-release fertilizers (SRFs)	231:283	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	0	56	theme	coating	75:81	arg1	material					83:90	an eco-friendly and multifunctional coating material	39:90	an eco-friendly and multifunctional coating material for slow-release fertilizers	39:119	Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.					
36063891	10	57	with	fertilizers	1808:1818	arg1	performances					1841:1852	multifunctional performances	1825:1852	multifunctional performances	1825:1852	These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.					
36063891	5	58	theme	Chemical	984:991	arg1	studies					1023:1029	Chemical, physical, and biodegradation studies	984:1029	Chemical, physical, and biodegradation studies	984:1029	Chemical, physical, and biodegradation studies have confirmed that the coating material is environmentally-friendly.					
36063891	6	59	theme	release	1111:1117	arg1	experiments					1119:1129	Nutrients release experiments	1101:1129	Nutrients release experiments in water as well as in soil environments	1101:1170	Nutrients release experiments in water as well as in soil environments have proved the effectiveness of the MC and MC/LGe coating layers in delaying the nutrients discharge.					
36063891	0	60	theme	multifunctional	59:73	arg1	material					83:90	an eco-friendly and multifunctional coating material	39:90	an eco-friendly and multifunctional coating material for slow-release fertilizers	39:119	Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.					
36063891	1	61	theme	biopolymers	336:346	arg1	use					329:331	the use	325:331	the use of biopolymers as coating material	325:366	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	5	62	theme	physical	994:1001	arg1	studies					1023:1029	Chemical, physical, and biodegradation studies	984:1029	Chemical, physical, and biodegradation studies	984:1029	Chemical, physical, and biodegradation studies have confirmed that the coating material is environmentally-friendly.					
36063891	10	63	theme	coating	1767:1773	arg1	material					1775:1782	an efficient coating material	1754:1782	an efficient coating material to produce slow-release fertilizers with multifunctional performances	1754:1852	These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.					
36063891	10	63	theme	coating	1767:1773	arg1	composite					1727:1735	this hybrid composite	1715:1735	this hybrid composite	1715:1735	These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.					
36063891	9	64	theme	roots	1635:1639	arg1	architecture					1641:1652	the roots architecture	1631:1652	the roots architecture	1631:1652	The agronomic evaluation has also confirmed their remarkable potential in enhancing wheat leaf area, chlorophyll content and biomass, in addition to the roots architecture and the final fruiting efficiency.					
36063891	6	65	theme	Nutrients	1101:1109	arg1	experiments					1119:1129	Nutrients release experiments	1101:1129	Nutrients release experiments in water as well as in soil environments	1101:1170	Nutrients release experiments in water as well as in soil environments have proved the effectiveness of the MC and MC/LGe coating layers in delaying the nutrients discharge.					
36063891	8	66	theme	fertilizers	1401:1411	arg1	resistance					1424:1433	the fertilizers mechanical resistance	1397:1433	the fertilizers mechanical resistance	1397:1433	Furthermore, the coating film enhanced the fertilizers mechanical resistance and boosted the soil water retention capacity.					
36063891	4	67	theme	Di-ammonium	871:881	arg1	DAP					894:896	DAP	894:896	DAP	894:896	Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine.					
36063891	4	67	theme	Di-ammonium	871:881	arg1	Phosphate					883:891	Di-ammonium Phosphate	871:891	Di-ammonium Phosphate (DAP)	871:897	Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine.					
36063891	10	68	theme	multifunctional	1825:1839	arg1	performances					1841:1852	multifunctional performances	1825:1852	multifunctional performances	1825:1852	These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.					
36063891	1	69	theme	fertilizers	266:276	arg1	non-biodegradability					207:226	the non-biodegradability	203:226	the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs)	203:283	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	10	70	theme	hybrid	1720:1725	arg1	composite					1727:1735	this hybrid composite	1715:1735	this hybrid composite	1715:1735	These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.					
36063891	10	70	theme	hybrid	1720:1725	arg1	material					1775:1782	an efficient coating material	1754:1782	an efficient coating material to produce slow-release fertilizers with multifunctional performances	1754:1852	These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.					
36063891	0	71	from	Effect	122:127	arg1	management					142:151	nutrients management	132:151	nutrients management	132:151	Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.					
36063891	0	71	from	Effect	122:127	arg1	growth					163:168	wheat growth	157:168	wheat growth	157:168	Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.					
36063891	8	72	theme	coating	1375:1381	arg1	film					1383:1386	the coating film	1371:1386	the coating film	1371:1386	Furthermore, the coating film enhanced the fertilizers mechanical resistance and boosted the soil water retention capacity.					
36063891	8	73	theme	retention	1462:1470	arg1	capacity					1472:1479	the soil water retention capacity	1447:1479	the soil water retention capacity	1447:1479	Furthermore, the coating film enhanced the fertilizers mechanical resistance and boosted the soil water retention capacity.					
36063891	6	74	from	experiments	1119:1129	arg1	water					1134:1138	water	1134:1138	water	1134:1138	Nutrients release experiments in water as well as in soil environments have proved the effectiveness of the MC and MC/LGe coating layers in delaying the nutrients discharge.					
36063891	6	74	from	experiments	1119:1129	arg1	environments					1159:1170	soil environments	1154:1170	soil environments	1154:1170	Nutrients release experiments in water as well as in soil environments have proved the effectiveness of the MC and MC/LGe coating layers in delaying the nutrients discharge.					
36063891	2	75	theme	present	426:432	arg1	work					434:437	The present work	422:437	The present work	422:437	The present work aims to design a sustainable coating material based on biodegradable polymers.					
36063891	0	76	dep	biocomposite	23:34	arg1	Effect					122:127	Effect	122:127	Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.	0:169	Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.					
36063891	9	77	theme	agronomic	1486:1494	arg1	evaluation					1496:1505	The agronomic evaluation	1482:1505	The agronomic evaluation	1482:1505	The agronomic evaluation has also confirmed their remarkable potential in enhancing wheat leaf area, chlorophyll content and biomass, in addition to the roots architecture and the final fruiting efficiency.					
36063891	3	78	theme	Alfa	531:534	arg1	source					590:595	a viable sustainable source	569:595	a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent	569:815	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	3	78	theme	Alfa	531:534	arg1	plant					536:540	Alfa plant	531:540	Alfa plant	531:540	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	3	79	theme	citric	775:780	arg1	CA					788:789	CA	788:789	CA	788:789	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	3	79	theme	citric	775:780	arg1	acid					782:785	citric acid	775:785	citric acid (CA)	775:790	To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent.					
36063891	4	80	theme	pan	971:973	arg1	machine					975:981	a rotating pan machine	960:981	a rotating pan machine	960:981	Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine.					
36063891	1	81	theme	coating	351:357	arg1	material					359:366	coating material	351:366	coating material	351:366	To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles.					
36063891	10	82	theme	slow-release	1795:1806	arg1	fertilizers					1808:1818	slow-release fertilizers	1795:1818	slow-release fertilizers with multifunctional performances	1795:1852	These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.					
36063891	9	83	dep	architecture	1641:1652	arg1	addition					1619:1626	addition	1619:1626	addition	1619:1626	The agronomic evaluation has also confirmed their remarkable potential in enhancing wheat leaf area, chlorophyll content and biomass, in addition to the roots architecture and the final fruiting efficiency.					
36063891	4	84	from	fertilizers	945:955	arg1	machine					975:981	a rotating pan machine	960:981	a rotating pan machine	960:981	Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine.					
35956904	3	0	theme	Cyclic	482:487	arg1	voltammetry					489:499	Cyclic voltammetry	482:499	Cyclic voltammetry (CV)	482:504	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	3	0	theme	Cyclic	482:487	arg1	CV					502:503	CV	502:503	CV	502:503	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	1	1	theme	selective	247:255	arg1	detection					257:265	highly sensitive and selective detection	226:265	highly sensitive and selective detection of Cu2+	226:273	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	5	2	theme	β-CD/CMK-8	747:756	arg1	electrode					767:775	the β-CD/CMK-8 modified electrode	743:775	the β-CD/CMK-8 modified electrode	743:775	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	2	3	dep	morphology	280:289	arg1	The					276:278	The	276:278	The	276:278	The morphology and structure of β-CD/CMK-8 were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD).					
35956904	3	4	from	voltammetry	489:499	arg1	dates					422:426	the dates	418:426	the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV)	418:504	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	5	5	theme	detection	858:866	arg1	limit					868:872	a low detection limit	852:872	a low detection limit of 0.3 ng·L-1	852:886	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	8	6	theme	sensitive	1099:1107	arg1	method					1109:1114	a simple and sensitive method	1086:1114	a simple and sensitive method	1086:1114	All in all, this work proposes a simple and sensitive method for Cu2+ detection, which provides a reference for the subsequent detection of HMIs.					
35956904	6	7	theme	β-CD/CMK-8	956:965	arg1	repeatability					906:918	repeatability	906:918	repeatability	906:918	Furthermore, the repeatability, reproducibility and selectivity of β-CD/CMK-8 towards Cu2+ were commendable.					
35956904	6	7	theme	β-CD/CMK-8	956:965	arg1	selectivity					941:951	selectivity	941:951	selectivity	941:951	Furthermore, the repeatability, reproducibility and selectivity of β-CD/CMK-8 towards Cu2+ were commendable.					
35956904	6	7	theme	β-CD/CMK-8	956:965	arg1	reproducibility					921:935	reproducibility	921:935	reproducibility	921:935	Furthermore, the repeatability, reproducibility and selectivity of β-CD/CMK-8 towards Cu2+ were commendable.					
35956904	1	8	theme	Cu2+	270:273	arg1	detection					257:265	highly sensitive and selective detection	226:265	highly sensitive and selective detection of Cu2+	226:273	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	8	9	theme	Cu2+	1120:1123	arg1	detection					1125:1133	Cu2+ detection	1120:1133	Cu2+ detection	1120:1133	All in all, this work proposes a simple and sensitive method for Cu2+ detection, which provides a reference for the subsequent detection of HMIs.					
35956904	5	10	theme	wide	788:791	arg1	result					706:711	a result	704:711	a result of these impressive features	704:740	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	5	10	theme	wide	788:791	arg1	response					800:807	a wide linear response	786:807	a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1	786:886	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	8	11	theme	HMIs	1195:1198	arg1	detection					1182:1190	the subsequent detection	1167:1190	the subsequent detection of HMIs	1167:1198	All in all, this work proposes a simple and sensitive method for Cu2+ detection, which provides a reference for the subsequent detection of HMIs.					
35956904	4	12	theme	β-CD/CMK-8	633:642	arg1	composite					644:652	the β-CD/CMK-8 composite	629:652	the β-CD/CMK-8 composite	629:652	Besides this, the β-CD/CMK-8 composite displayed high enrichment ability toward Cu2+.					
35956904	5	13	theme	impressive	722:731	arg1	features					733:740	these impressive features	716:740	these impressive features	716:740	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	5	14	theme	linear	793:798	arg1	result					706:711	a result	704:711	a result of these impressive features	704:740	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	5	14	theme	linear	793:798	arg1	response					800:807	a wide linear response	786:807	a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1	786:886	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	8	15	theme	subsequent	1171:1180	arg1	detection					1182:1190	the subsequent detection	1167:1190	the subsequent detection of HMIs	1167:1198	All in all, this work proposes a simple and sensitive method for Cu2+ detection, which provides a reference for the subsequent detection of HMIs.					
35956904	0	16	theme	Electrochemical	27:41	arg1	Sensor					43:48	β-Cyclodextrin/CMK-8-Based Electrochemical Sensor	0:48	β-Cyclodextrin/CMK-8-Based Electrochemical Sensor for Sensitive Detection of Cu2	0:79	β-Cyclodextrin/CMK-8-Based Electrochemical Sensor for Sensitive Detection of Cu2.					
35956904	8	17	theme	simple	1088:1093	arg1	method					1109:1114	a simple and sensitive method	1086:1114	a simple and sensitive method	1086:1114	All in all, this work proposes a simple and sensitive method for Cu2+ detection, which provides a reference for the subsequent detection of HMIs.					
35956904	2	18	theme	X-ray	380:384	arg1	diffraction					386:396	X-ray diffraction	380:396	X-ray diffraction (XRD)	380:402	The morphology and structure of β-CD/CMK-8 were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD).					
35956904	2	18	theme	X-ray	380:384	arg1	XRD					399:401	XRD	399:401	XRD	399:401	The morphology and structure of β-CD/CMK-8 were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD).					
35956904	0	19	theme	β-Cyclodextrin/CMK-8-Based	0:25	arg1	Sensor					43:48	β-Cyclodextrin/CMK-8-Based Electrochemical Sensor	0:48	β-Cyclodextrin/CMK-8-Based Electrochemical Sensor for Sensitive Detection of Cu2	0:79	β-Cyclodextrin/CMK-8-Based Electrochemical Sensor for Sensitive Detection of Cu2.					
35956904	3	20	contain	possessed	539:547	arg2	area					609:612	large effective surface area	585:612	large effective surface area	585:612	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	3	20	contain	possessed	539:547	arg1	β-CD/CMK-8					528:537	the β-CD/CMK-8	524:537	the β-CD/CMK-8	524:537	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	3	20	contain	possessed	539:547	arg2	rate					576:579	a fast electronic transfer rate	549:579	a fast electronic transfer rate	549:579	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	1	21	theme	β-cyclodextrin	96:109	arg1	nanocomposite					144:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	1	21	theme	β-cyclodextrin	96:109	arg1	platform					213:220	an electrochemical sensing platform	186:220	an electrochemical sensing platform for highly sensitive and selective detection of Cu2+	186:273	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	3	22	theme	impedance	449:457	arg1	EIS					473:475	EIS	473:475	EIS	473:475	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	3	22	theme	impedance	449:457	arg1	spectroscopy					459:470	electrochemical impedance spectroscopy	433:470	electrochemical impedance spectroscopy (EIS)	433:476	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	2	23	theme	β-CD/CMK-8	308:317	arg1	morphology					280:289	morphology	280:289	morphology	280:289	The morphology and structure of β-CD/CMK-8 were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD).					
35956904	2	23	theme	β-CD/CMK-8	308:317	arg1	structure					295:303	structure	295:303	structure	295:303	The morphology and structure of β-CD/CMK-8 were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD).					
35956904	7	24	theme	real	1041:1044	arg1	samples					1046:1052	real samples	1041:1052	real samples	1041:1052	The sensor could be used to detect Cu2+ in real samples.					
35956904	1	25	theme	β-CD	112:115	arg1	nanocomposite					144:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	1	25	theme	β-CD	112:115	arg1	platform					213:220	an electrochemical sensing platform	186:220	an electrochemical sensing platform for highly sensitive and selective detection of Cu2+	186:273	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	1	26	theme	electrochemical	189:203	arg1	nanocomposite					144:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	1	26	theme	electrochemical	189:203	arg1	platform					213:220	an electrochemical sensing platform	186:220	an electrochemical sensing platform for highly sensitive and selective detection of Cu2+	186:273	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	5	27	theme	modified	758:765	arg1	electrode					767:775	the β-CD/CMK-8 modified electrode	743:775	the β-CD/CMK-8 modified electrode	743:775	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	2	28	theme	scanning	341:348	arg1	SEM					371:373	SEM	371:373	SEM	371:373	The morphology and structure of β-CD/CMK-8 were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD).					
35956904	2	28	theme	scanning	341:348	arg1	microscope					359:368	scanning electron microscope	341:368	scanning electron microscope (SEM)	341:374	The morphology and structure of β-CD/CMK-8 were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD).					
35956904	1	29	theme	sensing	205:211	arg1	nanocomposite					144:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	1	29	theme	sensing	205:211	arg1	platform					213:220	an electrochemical sensing platform	186:220	an electrochemical sensing platform for highly sensitive and selective detection of Cu2+	186:273	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	0	30	theme	Sensitive	54:62	arg1	Detection					64:72	Sensitive Detection	54:72	Sensitive Detection of Cu2	54:79	β-Cyclodextrin/CMK-8-Based Electrochemical Sensor for Sensitive Detection of Cu2.					
35956904	5	31	theme	ng·L-1	881:886	arg1	limit					868:872	a low detection limit	852:872	a low detection limit of 0.3 ng·L-1	852:886	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	3	32	theme	electrochemical	433:447	arg1	EIS					473:475	EIS	473:475	EIS	473:475	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	3	32	theme	electrochemical	433:447	arg1	spectroscopy					459:470	electrochemical impedance spectroscopy	433:470	electrochemical impedance spectroscopy (EIS)	433:476	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	0	33	theme	Cu2	77:79	arg1	Detection					64:72	Sensitive Detection	54:72	Sensitive Detection of Cu2	54:79	β-Cyclodextrin/CMK-8-Based Electrochemical Sensor for Sensitive Detection of Cu2.					
35956904	5	34	theme	low	854:856	arg1	limit					868:872	a low detection limit	852:872	a low detection limit of 0.3 ng·L-1	852:886	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	6	35	dep	repeatability	906:918	arg1	the					902:904	the	902:904	the	902:904	Furthermore, the repeatability, reproducibility and selectivity of β-CD/CMK-8 towards Cu2+ were commendable.					
35956904	3	36	theme	electronic	556:565	arg1	rate					576:579	a fast electronic transfer rate	549:579	a fast electronic transfer rate	549:579	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	2	37	theme	electron	350:357	arg1	SEM					371:373	SEM	371:373	SEM	371:373	The morphology and structure of β-CD/CMK-8 were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD).					
35956904	2	37	theme	electron	350:357	arg1	microscope					359:368	scanning electron microscope	341:368	scanning electron microscope (SEM)	341:374	The morphology and structure of β-CD/CMK-8 were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD).					
35956904	7	38	used	used	1018:1021	arg2	sensor					1002:1007	The sensor	998:1007	The sensor	998:1007	The sensor could be used to detect Cu2+ in real samples.					
35956904	3	39	theme	transfer	567:574	arg1	rate					576:579	a fast electronic transfer rate	549:579	a fast electronic transfer rate	549:579	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	1	40	theme	/mesoporous	117:127	arg1	nanocomposite					144:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	1	40	theme	/mesoporous	117:127	arg1	platform					213:220	an electrochemical sensing platform	186:220	an electrochemical sensing platform for highly sensitive and selective detection of Cu2+	186:273	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	3	41	theme	surface	601:607	arg1	area					609:612	large effective surface area	585:612	large effective surface area	585:612	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	3	42	from	spectroscopy	459:470	arg1	dates					422:426	the dates	418:426	the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV)	418:504	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	1	43	theme	carbon	129:134	arg1	nanocomposite					144:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	1	43	theme	carbon	129:134	arg1	platform					213:220	an electrochemical sensing platform	186:220	an electrochemical sensing platform for highly sensitive and selective detection of Cu2+	186:273	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	5	44	with	mg·L-1	840:845	arg1	limit					868:872	a low detection limit	852:872	a low detection limit of 0.3 ng·L-1	852:886	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	1	45	used	used	178:181	arg2	platform					213:220	an electrochemical sensing platform	186:220	an electrochemical sensing platform for highly sensitive and selective detection of Cu2+	186:273	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	1	45	used	used	178:181	arg2	nanocomposite					144:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite	96:156	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	4	46	theme	high	664:667	arg1	ability					680:686	high enrichment ability	664:686	high enrichment ability	664:686	Besides this, the β-CD/CMK-8 composite displayed high enrichment ability toward Cu2+.					
35956904	5	47	theme	features	733:740	arg1	result					706:711	a result	704:711	a result of these impressive features	704:740	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	5	47	theme	features	733:740	arg1	response					800:807	a wide linear response	786:807	a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1	786:886	As a result of these impressive features, the β-CD/CMK-8 modified electrode provided a wide linear response ranging from 0.1 ng·L-1 to 1.0 mg·L-1 with a low detection limit of 0.3 ng·L-1.					
35956904	3	48	theme	large	585:589	arg1	area					609:612	large effective surface area	585:612	large effective surface area	585:612	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	4	49	theme	enrichment	669:678	arg1	ability					680:686	high enrichment ability	664:686	high enrichment ability	664:686	Besides this, the β-CD/CMK-8 composite displayed high enrichment ability toward Cu2+.					
35956904	1	50	theme	sensitive	233:241	arg1	detection					257:265	highly sensitive and selective detection	226:265	highly sensitive and selective detection of Cu2+	226:273	In this work, β-cyclodextrin (β-CD)/mesoporous carbon (CMK-8) nanocomposite was synthesized and used as an electrochemical sensing platform for highly sensitive and selective detection of Cu2+.					
35956904	3	51	theme	effective	591:599	arg1	area					609:612	large effective surface area	585:612	large effective surface area	585:612	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
35956904	3	52	theme	fast	551:554	arg1	rate					576:579	a fast electronic transfer rate	549:579	a fast electronic transfer rate	549:579	In addition, the dates from electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV) demonstrated that the β-CD/CMK-8 possessed a fast electronic transfer rate and large effective surface area.					
36812968	7	0	theme	scaffold	1265:1272	arg1	Proliferation					1235:1247	Proliferation	1235:1247	Proliferation of the membrane scaffold	1235:1272	Proliferation of the membrane scaffold was 189.83 % at 24 h and 209.12 % at 72 h.					
36812968	8	1	from	end	1349:1351	arg1	test					1336:1339	the in vivo rat test;	1320:1340	the in vivo rat test; at the end of 28th day	1320:1363	In the in vivo rat test; at the end of 28th day, it was found that biomembrane_3 provided 98.75 ± 0.12 % wound shrinkage.					
36812968	5	2	dep	In	873:874	arg1	vitro					876:880	vitro	876:880	vitro	876:880	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	2	3	theme	Bioactive	233:241	arg1	biomaterials					278:289	Bioactive, antioxidant Fucoidan and Chitosan biomaterials	233:289	Bioactive, antioxidant Fucoidan and Chitosan biomaterials	233:289	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	2	4	with	design	449:454	arg1	properties					488:497	suitable cell regeneration properties	461:497	suitable cell regeneration properties	461:497	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	4	5	theme	Analysis	722:729	arg1	analyses					780:787	Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses	673:787	Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses	673:787	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	5	6	theme	structures	943:952	arg1	modelling					911:919	In vitro Franz diffusion mathematical modelling	873:919	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests	873:1003	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	7	7	from	24 h	1290:1293	arg1	%					1306:1306	209.12 %	1299:1306	209.12 % at 72 h	1299:1314	Proliferation of the membrane scaffold was 189.83 % at 24 h and 209.12 % at 72 h.					
36812968	7	7	from	24 h	1290:1293	arg1	%					1285:1285	189.83 %	1278:1285	189.83 % at 24 h	1278:1293	Proliferation of the membrane scaffold was 189.83 % at 24 h and 209.12 % at 72 h.					
36812968	2	8	theme	based	348:352	arg1	hydrogels					364:372	polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels	307:372	polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties	307:430	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	1	9	theme	wound	191:195	arg1	applications					206:217	wound dressing applications	191:217	wound dressing applications	191:217	In this article, the suitability of composite transdermal biomaterial for wound dressing applications is discussed.					
36812968	10	10	theme	transdermal	1743:1753	arg1	biomaterial					1755:1765	the innovative and novel transdermal biomaterial	1718:1765	the innovative and novel transdermal biomaterial	1718:1765	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	2	11	dep	Bioactive	233:241	arg1	antioxidant					244:254	antioxidant	244:254	antioxidant	244:254	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	3	12	theme	bioadhesion	595:605	arg1	properties					607:616	the bioadhesion properties	591:616	the bioadhesion properties of composite polymeric biomembranes	591:652	In accordance with this purpose, tissue profile analysis (TPA) was performed for the bioadhesion properties of composite polymeric biomembranes.					
36812968	8	13	located	found	1373:1377	arg1	test					1336:1339	the in vivo rat test;	1320:1340	the in vivo rat test; at the end of 28th day	1320:1363	In the in vivo rat test; at the end of 28th day, it was found that biomembrane_3 provided 98.75 ± 0.12 % wound shrinkage.					
36812968	8	13	located	found	1373:1377	arg2	it					1366:1367	it	1366:1367	it	1366:1367	In the in vivo rat test; at the end of 28th day, it was found that biomembrane_3 provided 98.75 ± 0.12 % wound shrinkage.					
36812968	6	14	theme	TPA	1021:1023	arg1	analysis					1025:1032	TPA analysis	1021:1032	TPA analysis of resveratrol	1021:1047	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	9	15	theme	statistical	1659:1669	arg1	analysis					1671:1678	Minitab statistical analysis	1651:1678	Minitab statistical analysis	1651:1678	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	8	16	theme	98.75 ± 0.12 	1407:1419	arg1	%					1420:1420	%	1420:1420	%	1420:1420	In the in vivo rat test; at the end of 28th day, it was found that biomembrane_3 provided 98.75 ± 0.12 % wound shrinkage.					
36812968	5	17	theme	composite	924:932	arg1	structures					943:952	composite membrane structures	924:952	composite membrane structures	924:952	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	8	18	theme	wound	1422:1426	arg1	shrinkage					1428:1436	98.75 ± 0.12 % wound shrinkage	1407:1436	98.75 ± 0.12 % wound shrinkage	1407:1436	In the in vivo rat test; at the end of 28th day, it was found that biomembrane_3 provided 98.75 ± 0.12 % wound shrinkage.					
36812968	3	19	theme	polymeric	631:639	arg1	biomembranes					641:652	composite polymeric biomembranes	621:652	composite polymeric biomembranes	621:652	In accordance with this purpose, tissue profile analysis (TPA) was performed for the bioadhesion properties of composite polymeric biomembranes.					
36812968	5	20	theme	in	987:988	arg1	tests					999:1003	in vivo rat tests	987:1003	in vivo rat tests	987:1003	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	2	21	theme	Chitosan	269:276	arg1	biomaterials					278:289	Bioactive, antioxidant Fucoidan and Chitosan biomaterials	233:289	Bioactive, antioxidant Fucoidan and Chitosan biomaterials	233:289	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	9	22	theme	transdermal	1468:1478	arg1	scaffold					1489:1496	the transdermal membrane scaffold	1464:1496	the transdermal membrane scaffold	1464:1496	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	7	23	theme	membrane	1256:1263	arg1	scaffold					1265:1272	the membrane scaffold	1252:1272	the membrane scaffold	1252:1272	Proliferation of the membrane scaffold was 189.83 % at 24 h and 209.12 % at 72 h.					
36812968	2	24	theme	Fucoidan	256:263	arg1	biomaterials					278:289	Bioactive, antioxidant Fucoidan and Chitosan biomaterials	233:289	Bioactive, antioxidant Fucoidan and Chitosan biomaterials	233:289	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	5	25	theme	MTT	973:975	arg1	test					977:980	MTT test	973:980	MTT test	973:980	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	5	25	theme	MTT	973:975	arg1	biocompatibility					955:970	biocompatibility	955:970	biocompatibility (MTT test)	955:981	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	4	26	theme	Electron	750:757	arg1	SEM-EDS					771:777	SEM-EDS	771:777	SEM-EDS	771:777	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	4	26	theme	Electron	750:757	arg1	Microscopy					759:768	Scanning Electron Microscopy	741:768	Scanning Electron Microscopy (SEM-EDS)	741:778	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	0	27	theme	resveratrol	66:76	arg1	study					57:61	shelf-life study	46:61	shelf-life study of resveratrol	46:76	Release kinetics modelling and in vivo-vitro, shelf-life study of resveratrol added composite transdermal scaffolds.					
36812968	1	28	theme	composite	153:161	arg1	biomaterial					175:185	composite transdermal biomaterial	153:185	composite transdermal biomaterial	153:185	In this article, the suitability of composite transdermal biomaterial for wound dressing applications is discussed.					
36812968	6	29	theme	scaffold	1068:1075	arg1	design					1077:1082	biomembrane scaffold design	1056:1082	biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness	1056:1208	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	0	30	theme	composite	84:92	arg1	scaffolds					106:114	composite transdermal scaffolds	84:114	composite transdermal scaffolds	84:114	Release kinetics modelling and in vivo-vitro, shelf-life study of resveratrol added composite transdermal scaffolds.					
36812968	5	31	theme	rat	995:997	arg1	tests					999:1003	in vivo rat tests	987:1003	in vivo rat tests	987:1003	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	2	32	theme	biomembrane	437:447	arg1	design					449:454	biomembrane design	437:454	biomembrane design with suitable cell regeneration properties	437:497	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	2	33	contain	has	405:407	arg2	properties					421:430	therapeutic properties	409:430	therapeutic properties	409:430	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	2	33	contain	has	405:407	arg1	Resveratrol					386:396	Resveratrol	386:396	Resveratrol	386:396	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	0	34	theme	Release	0:6	arg1	kinetics					8:15	Release kinetics	0:15	Release kinetics	0:15	Release kinetics modelling and in vivo-vitro, shelf-life study of resveratrol added composite transdermal scaffolds.					
36812968	10	35	from	regeneration	1788:1799	arg1	applications					1838:1849	biomedical applications	1827:1849	biomedical applications	1827:1849	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	8	36	theme	28th	1356:1359	arg1	day					1361:1363	28th day	1356:1363	28th day	1356:1363	In the in vivo rat test; at the end of 28th day, it was found that biomembrane_3 provided 98.75 ± 0.12 % wound shrinkage.					
36812968	2	37	theme	cell	470:473	arg1	properties					488:497	suitable cell regeneration properties	461:497	suitable cell regeneration properties	461:497	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	5	38	theme	diffusion	888:896	arg1	modelling					911:919	In vitro Franz diffusion mathematical modelling	873:919	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests	873:1003	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	10	39	theme	biomedical	1827:1836	arg1	applications					1838:1849	biomedical applications	1827:1849	biomedical applications	1827:1849	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	1	40	theme	transdermal	163:173	arg1	biomaterial					175:185	composite transdermal biomaterial	153:185	composite transdermal biomaterial	153:185	In this article, the suitability of composite transdermal biomaterial for wound dressing applications is discussed.					
36812968	8	41	dep	in	1324:1325	arg1	vivo					1327:1330	vivo	1327:1330	vivo	1327:1330	In the in vivo rat test; at the end of 28th day, it was found that biomembrane_3 provided 98.75 ± 0.12 % wound shrinkage.					
36812968	9	42	from	shelf-life	1443:1452	arg1	scaffold					1489:1496	the transdermal membrane scaffold	1464:1496	the transdermal membrane scaffold	1464:1496	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	9	43	theme	in	1561:1562	arg1	modelling					1599:1607	in vitro Franz diffusion mathematical modelling	1561:1607	in vitro Franz diffusion mathematical modelling	1561:1607	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	7	44	from	72 h	1311:1314	arg1	%					1306:1306	209.12 %	1299:1306	209.12 % at 72 h	1299:1314	Proliferation of the membrane scaffold was 189.83 % at 24 h and 209.12 % at 72 h.					
36812968	7	44	from	72 h	1311:1314	arg1	%					1285:1285	189.83 %	1278:1285	189.83 % at 24 h	1278:1293	Proliferation of the membrane scaffold was 189.83 % at 24 h and 209.12 % at 72 h.					
36812968	10	45	theme	cell	1783:1786	arg1	regeneration					1788:1799	tissue cell regeneration	1776:1799	tissue cell regeneration	1776:1799	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	10	45	theme	cell	1783:1786	arg1	proliferation					1810:1822	cell proliferation	1805:1822	cell proliferation	1805:1822	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	10	45	theme	cell	1783:1786	arg1	dressing					1862:1869	a wound dressing	1854:1869	a wound dressing	1854:1869	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	10	46	theme	wound	1856:1860	arg1	regeneration					1788:1799	tissue cell regeneration	1776:1799	tissue cell regeneration	1776:1799	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	10	46	theme	wound	1856:1860	arg1	proliferation					1810:1822	cell proliferation	1805:1822	cell proliferation	1805:1822	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	10	46	theme	wound	1856:1860	arg1	dressing					1862:1869	a wound dressing	1854:1869	a wound dressing	1854:1869	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	4	47	theme	biomembrane	849:859	arg1	structures					861:870	biomembrane structures	849:870	biomembrane structures	849:870	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	9	48	theme	Franz	1570:1574	arg1	modelling					1599:1607	in vitro Franz diffusion mathematical modelling	1561:1607	in vitro Franz diffusion mathematical modelling	1561:1607	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	2	49	theme	therapeutic	409:419	arg1	properties					421:430	therapeutic properties	409:430	therapeutic properties	409:430	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	1	50	theme	biomaterial	175:185	arg1	suitability					138:148	the suitability	134:148	the suitability of composite transdermal biomaterial for wound dressing applications	134:217	In this article, the suitability of composite transdermal biomaterial for wound dressing applications is discussed.					
36812968	10	51	from	proliferation	1810:1822	arg1	applications					1838:1849	biomedical applications	1827:1849	biomedical applications	1827:1849	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	9	52	theme	mathematical	1586:1597	arg1	modelling					1599:1607	in vitro Franz diffusion mathematical modelling	1561:1607	in vitro Franz diffusion mathematical modelling	1561:1607	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	4	53	theme	Infrared	673:680	arg1	analyses					780:787	Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses	673:787	Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses	673:787	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	3	54	theme	profile	550:556	arg1	analysis					558:565	tissue profile analysis	543:565	tissue profile analysis (TPA)	543:571	In accordance with this purpose, tissue profile analysis (TPA) was performed for the bioadhesion properties of composite polymeric biomembranes.					
36812968	3	54	theme	profile	550:556	arg1	TPA					568:570	TPA	568:570	TPA	568:570	In accordance with this purpose, tissue profile analysis (TPA) was performed for the bioadhesion properties of composite polymeric biomembranes.					
36812968	2	55	theme	polyvinyl	307:315	arg1	hydrogels					364:372	polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels	307:372	polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties	307:430	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	10	56	theme	novel	1737:1741	arg1	biomaterial					1755:1765	the innovative and novel transdermal biomaterial	1718:1765	the innovative and novel transdermal biomaterial	1718:1765	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	10	57	theme	study	1704:1708	arg1	importance					1685:1694	The importance	1681:1694	The importance of this study	1681:1708	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	5	58	theme	membrane	934:941	arg1	structures					943:952	composite membrane structures	924:952	composite membrane structures	924:952	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	4	59	theme	Thermogravimetric	704:720	arg1	Analysis					722:729	Thermogravimetric Analysis	704:729	Thermogravimetric Analysis (TGA)	704:735	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	4	59	theme	Thermogravimetric	704:720	arg1	TGA					732:734	TGA	732:734	TGA	732:734	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	2	60	theme	polymeric	354:362	arg1	hydrogels					364:372	polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels	307:372	polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties	307:430	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	6	61	theme	resveratrol	1037:1047	arg1	analysis					1025:1032	TPA analysis	1021:1032	TPA analysis of resveratrol	1021:1047	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	2	62	theme	phosphate	338:346	arg1	hydrogels					364:372	polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels	307:372	polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties	307:430	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	9	63	dep	in	1561:1562	arg1	vitro					1564:1568	vitro	1564:1568	vitro	1564:1568	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	5	64	theme	mathematical	898:909	arg1	modelling					911:919	In vitro Franz diffusion mathematical modelling	873:919	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests	873:1003	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	9	65	theme	Minitab	1651:1657	arg1	analysis					1671:1678	Minitab statistical analysis	1651:1678	Minitab statistical analysis	1651:1678	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	8	66	theme	%	1420:1420	arg1	shrinkage					1428:1436	98.75 ± 0.12 % wound shrinkage	1407:1436	98.75 ± 0.12 % wound shrinkage	1407:1436	In the in vivo rat test; at the end of 28th day, it was found that biomembrane_3 provided 98.75 ± 0.12 % wound shrinkage.					
36812968	4	67	theme	Scanning	741:748	arg1	SEM-EDS					771:777	SEM-EDS	771:777	SEM-EDS	771:777	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	4	67	theme	Scanning	741:748	arg1	Microscopy					759:768	Scanning Electron Microscopy	741:768	Scanning Electron Microscopy (SEM-EDS)	741:778	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	3	68	theme	biomembranes	641:652	arg1	properties					607:616	the bioadhesion properties	591:616	the bioadhesion properties of composite polymeric biomembranes	591:652	In accordance with this purpose, tissue profile analysis (TPA) was performed for the bioadhesion properties of composite polymeric biomembranes.					
36812968	5	69	theme	biocompatibility	955:970	arg1	modelling					911:919	In vitro Franz diffusion mathematical modelling	873:919	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests	873:1003	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	3	70	theme	composite	621:629	arg1	biomembranes					641:652	composite polymeric biomembranes	621:652	composite polymeric biomembranes	621:652	In accordance with this purpose, tissue profile analysis (TPA) was performed for the bioadhesion properties of composite polymeric biomembranes.					
36812968	9	71	theme	RES	1457:1459	arg1	shelf-life					1443:1452	The shelf-life	1439:1452	The shelf-life	1439:1452	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	9	71	theme	RES	1457:1459	arg1	order					1528:1532	Zero order	1523:1532	Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling	1523:1607	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	9	71	theme	RES	1457:1459	arg1	35 days					1640:1646	35 days	1640:1646	35 days	1640:1646	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	4	72	theme	Microscopy	759:768	arg1	analyses					780:787	Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses	673:787	Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses	673:787	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	10	73	theme	cell	1805:1808	arg1	regeneration					1788:1799	tissue cell regeneration	1776:1799	tissue cell regeneration	1776:1799	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	10	73	theme	cell	1805:1808	arg1	proliferation					1810:1822	cell proliferation	1805:1822	cell proliferation	1805:1822	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	10	73	theme	cell	1805:1808	arg1	dressing					1862:1869	a wound dressing	1854:1869	a wound dressing	1854:1869	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	10	74	theme	innovative	1722:1731	arg1	biomaterial					1755:1765	the innovative and novel transdermal biomaterial	1718:1765	the innovative and novel transdermal biomaterial	1718:1765	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	4	75	dep	Fourier	655:661	arg1	Transform					663:671	Transform	663:671	Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses	663:787	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	1	76	theme	dressing	197:204	arg1	applications					206:217	wound dressing applications	191:217	wound dressing applications	191:217	In this article, the suitability of composite transdermal biomaterial for wound dressing applications is discussed.					
36812968	9	77	theme	membrane	1480:1487	arg1	scaffold					1489:1496	the transdermal membrane scaffold	1464:1496	the transdermal membrane scaffold	1464:1496	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	6	78	dep	design	1077:1082	arg1	elasticity					1172:1181	elasticity	1172:1181	elasticity	1172:1181	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	6	78	dep	design	1077:1082	arg1	compressibility					1085:1099	compressibility	1085:1099	compressibility	1085:1099	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	6	78	dep	design	1077:1082	arg1	adhesiveness					1142:1153	adhesiveness	1142:1153	adhesiveness	1142:1153	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	6	78	dep	design	1077:1082	arg1	cohesiveness					1197:1208	cohesiveness	1197:1208	cohesiveness	1197:1208	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	6	78	dep	design	1077:1082	arg1	hardness					1119:1126	hardness	1119:1126	hardness	1119:1126	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	8	79	theme	in	1324:1325	arg1	test					1336:1339	the in vivo rat test;	1320:1340	the in vivo rat test; at the end of 28th day	1320:1363	In the in vivo rat test; at the end of 28th day, it was found that biomembrane_3 provided 98.75 ± 0.12 % wound shrinkage.					
36812968	0	80	theme	transdermal	94:104	arg1	scaffolds					106:114	composite transdermal scaffolds	84:114	composite transdermal scaffolds	84:114	Release kinetics modelling and in vivo-vitro, shelf-life study of resveratrol added composite transdermal scaffolds.					
36812968	0	81	dep	modelling	17:25	arg1	added					78:82	added	78:82	added composite transdermal scaffolds	78:114	Release kinetics modelling and in vivo-vitro, shelf-life study of resveratrol added composite transdermal scaffolds.					
36812968	2	82	theme	alcohol/β-tricalcium	317:336	arg1	hydrogels					364:372	polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels	307:372	polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties	307:430	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	6	83	theme	biomembrane	1056:1066	arg1	design					1077:1082	biomembrane scaffold design	1056:1082	biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness	1056:1208	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	6	84	dep	compressibility	1085:1099	arg1	-11 ± 2.0					1156:1164	-11 ± 2.0	1156:1164	-11 ± 2.0	1156:1164	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	6	84	dep	compressibility	1085:1099	arg1	g.s					1113:1115	g.s	1113:1115	g.s	1113:1115	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	6	84	dep	compressibility	1085:1099	arg1	0.61 ± 0.07					1184:1194	0.61 ± 0.07	1184:1194	0.61 ± 0.07	1184:1194	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	6	84	dep	compressibility	1085:1099	arg1	g.s					1166:1168	g.s	1166:1168	g.s	1166:1168	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	6	84	dep	compressibility	1085:1099	arg1	g					1138:1138	g	1138:1138	g	1138:1138	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	6	84	dep	compressibility	1085:1099	arg1	13.4 ± 1.9					1102:1111	13.4 ± 1.9	1102:1111	13.4 ± 1.9	1102:1111	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	6	84	dep	compressibility	1085:1099	arg1	16.8 ± 1					1129:1136	16.8 ± 1	1129:1136	16.8 ± 1(g)	1129:1139	TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; -11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found.					
36812968	8	85	theme	rat	1332:1334	arg1	test					1336:1339	the in vivo rat test;	1320:1340	the in vivo rat test; at the end of 28th day	1320:1363	In the in vivo rat test; at the end of 28th day, it was found that biomembrane_3 provided 98.75 ± 0.12 % wound shrinkage.					
36812968	4	86	theme	morphological	808:820	arg1	analyses					837:844	morphological and structural analyses	808:844	morphological and structural analyses of biomembrane structures	808:870	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	4	87	theme	structural	826:835	arg1	analyses					837:844	morphological and structural analyses	808:844	morphological and structural analyses of biomembrane structures	808:870	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	5	88	theme	In	873:874	arg1	modelling					911:919	In vitro Franz diffusion mathematical modelling	873:919	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests	873:1003	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	5	89	theme	tests	999:1003	arg1	modelling					911:919	In vitro Franz diffusion mathematical modelling	873:919	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests	873:1003	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	5	90	theme	Franz	882:886	arg1	modelling					911:919	In vitro Franz diffusion mathematical modelling	873:919	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests	873:1003	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	10	91	theme	tissue	1776:1781	arg1	regeneration					1788:1799	tissue cell regeneration	1776:1799	tissue cell regeneration	1776:1799	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	10	91	theme	tissue	1776:1781	arg1	proliferation					1810:1822	cell proliferation	1805:1822	cell proliferation	1805:1822	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	10	91	theme	tissue	1776:1781	arg1	dressing					1862:1869	a wound dressing	1854:1869	a wound dressing	1854:1869	The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in biomedical applications as a wound dressing.					
36812968	2	92	theme	regeneration	475:486	arg1	properties					488:497	suitable cell regeneration properties	461:497	suitable cell regeneration properties	461:497	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	2	93	theme	suitable	461:468	arg1	properties					488:497	suitable cell regeneration properties	461:497	suitable cell regeneration properties	461:497	Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has therapeutic properties, and biomembrane design with suitable cell regeneration properties was aimed.					
36812968	9	94	from	law	1554:1556	arg1	modelling					1599:1607	in vitro Franz diffusion mathematical modelling	1561:1607	in vitro Franz diffusion mathematical modelling	1561:1607	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	8	95	theme	day	1361:1363	arg1	end					1349:1351	the end	1345:1351	the end of 28th day	1345:1363	In the in vivo rat test; at the end of 28th day, it was found that biomembrane_3 provided 98.75 ± 0.12 % wound shrinkage.					
36812968	0	96	theme	shelf-life	46:55	arg1	study					57:61	shelf-life study	46:61	shelf-life study of resveratrol	46:76	Release kinetics modelling and in vivo-vitro, shelf-life study of resveratrol added composite transdermal scaffolds.					
36812968	3	97	theme	tissue	543:548	arg1	analysis					558:565	tissue profile analysis	543:565	tissue profile analysis (TPA)	543:571	In accordance with this purpose, tissue profile analysis (TPA) was performed for the bioadhesion properties of composite polymeric biomembranes.					
36812968	3	97	theme	tissue	543:548	arg1	TPA					568:570	TPA	568:570	TPA	568:570	In accordance with this purpose, tissue profile analysis (TPA) was performed for the bioadhesion properties of composite polymeric biomembranes.					
36812968	5	98	dep	in	987:988	arg1	vivo					990:993	vivo	990:993	vivo	990:993	In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed.					
36812968	9	99	theme	diffusion	1576:1584	arg1	modelling					1599:1607	in vitro Franz diffusion mathematical modelling	1561:1607	in vitro Franz diffusion mathematical modelling	1561:1607	The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis.					
36812968	4	100	theme	Spectrometry	682:693	arg1	analyses					780:787	Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses	673:787	Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses	673:787	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
36812968	4	101	theme	structures	861:870	arg1	analyses					837:844	morphological and structural analyses	808:844	morphological and structural analyses of biomembrane structures	808:870	Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures.					
37268075	4	0	theme	adsorbents	703:712	arg1	structure					686:694	structure	686:694	structure	686:694	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	4	0	theme	adsorbents	703:712	arg1	morphology					671:680	morphology	671:680	morphology	671:680	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	1	1	theme	membrane	164:171	arg1	shape					153:157	the shape	149:157	the shape of a membrane for the rapid removal of fluoride from water	149:216	A novel and easily separable adsorbent in the shape of a membrane for the rapid removal of fluoride from water was prepared after testing Zr, La and LaZr to modify a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr).					
37268075	6	2	theme	environmentally	1113:1127	arg1	CS/PVA-La-Zr					1138:1149	an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr	1080:1149	an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr	1080:1149	This study showed that an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr has the potential to remove fluoride effectively from drinking water in a short time.					
37268075	6	3	theme	short	1225:1229	arg1	time					1231:1234	a short time	1223:1234	a short time	1223:1234	This study showed that an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr has the potential to remove fluoride effectively from drinking water in a short time.					
37268075	3	4	theme	fluoride	528:535	arg1	behavior					548:555	The fluoride adsorption behavior	524:555	The fluoride adsorption behavior of the CS/PVA-La-Zr composite	524:585	The fluoride adsorption behavior of the CS/PVA-La-Zr composite can be described by pseudo-second-order kinetics and Langmuir isotherms models.					
37268075	5	5	dep	hydroxide	1028:1036	arg1	ions					1051:1054	ions	1051:1054	ions	1051:1054	The adsorption mechanism was studied using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), and which showed that ion exchange occurred mainly with hydroxide and fluoride ions.					
37268075	4	6	theme	dispersive	779:788	arg1	EDS					804:806	EDS	804:806	EDS	804:806	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	4	6	theme	dispersive	779:788	arg1	spectroscopy					790:801	energy dispersive spectroscopy	772:801	energy dispersive spectroscopy (EDS)	772:807	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	5	7	theme	adsorption	842:851	arg1	mechanism					853:861	The adsorption mechanism	838:861	The adsorption mechanism	838:861	The adsorption mechanism was studied using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), and which showed that ion exchange occurred mainly with hydroxide and fluoride ions.					
37268075	6	8	contain	has	1151:1153	arg1	CS/PVA-La-Zr					1138:1149	an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr	1080:1149	an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr	1080:1149	This study showed that an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr has the potential to remove fluoride effectively from drinking water in a short time.					
37268075	6	8	contain	has	1151:1153	arg2	potential					1159:1167	the potential to remove fluoride effectively from drinking water in a short time	1155:1234	the potential to remove fluoride effectively from drinking water in a short time	1155:1234	This study showed that an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr has the potential to remove fluoride effectively from drinking water in a short time.					
37268075	6	9	theme	friendly	1129:1136	arg1	CS/PVA-La-Zr					1138:1149	an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr	1080:1149	an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr	1080:1149	This study showed that an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr has the potential to remove fluoride effectively from drinking water in a short time.					
37268075	4	10	theme	energy	772:777	arg1	EDS					804:806	EDS	804:806	EDS	804:806	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	4	10	theme	energy	772:777	arg1	spectroscopy					790:801	energy dispersive spectroscopy	772:801	energy dispersive spectroscopy (EDS)	772:807	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	1	11	theme	rapid	181:185	arg1	removal					187:193	the rapid removal	177:193	the rapid removal of fluoride from water	177:216	A novel and easily separable adsorbent in the shape of a membrane for the rapid removal of fluoride from water was prepared after testing Zr, La and LaZr to modify a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr).					
37268075	0	12	theme	chitosan/polyvinyl	29:46	arg1	composite					56:64	Zirconium/lanthanum-modified chitosan/polyvinyl alcohol composite	0:64	Zirconium/lanthanum-modified chitosan/polyvinyl alcohol composite	0:64	Zirconium/lanthanum-modified chitosan/polyvinyl alcohol composite adsorbent for rapid removal of fluoride.					
37268075	1	13	dep	composite	300:308	arg1	CS/PVA-La					332:340	CS/PVA-La	332:340	CS/PVA-La	332:340	A novel and easily separable adsorbent in the shape of a membrane for the rapid removal of fluoride from water was prepared after testing Zr, La and LaZr to modify a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr).					
37268075	1	13	dep	composite	300:308	arg1	CS/PVA-Zr					321:329	CS/PVA-Zr	321:329	CS/PVA-Zr	321:329	A novel and easily separable adsorbent in the shape of a membrane for the rapid removal of fluoride from water was prepared after testing Zr, La and LaZr to modify a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr).					
37268075	1	13	dep	composite	300:308	arg1	CS/PVA-LA-Zr					343:354	CS/PVA-LA-Zr	343:354	CS/PVA-LA-Zr	343:354	A novel and easily separable adsorbent in the shape of a membrane for the rapid removal of fluoride from water was prepared after testing Zr, La and LaZr to modify a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr).					
37268075	1	14	from	shape	153:157	arg1	adsorbent					136:144	adsorbent	136:144	adsorbent	136:144	A novel and easily separable adsorbent in the shape of a membrane for the rapid removal of fluoride from water was prepared after testing Zr, La and LaZr to modify a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr).					
37268075	5	15	dep	transform	889:897	arg1	infrared					899:906	infrared	899:906	transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS)	889:969	The adsorption mechanism was studied using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), and which showed that ion exchange occurred mainly with hydroxide and fluoride ions.					
37268075	0	16	theme	Zirconium/lanthanum-modified	0:27	arg1	composite					56:64	Zirconium/lanthanum-modified chitosan/polyvinyl alcohol composite	0:64	Zirconium/lanthanum-modified chitosan/polyvinyl alcohol composite	0:64	Zirconium/lanthanum-modified chitosan/polyvinyl alcohol composite adsorbent for rapid removal of fluoride.					
37268075	1	17	from	adsorbent	136:144	arg1	shape					153:157	the shape	149:157	the shape of a membrane for the rapid removal of fluoride from water	149:216	A novel and easily separable adsorbent in the shape of a membrane for the rapid removal of fluoride from water was prepared after testing Zr, La and LaZr to modify a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr).					
37268075	4	18	theme	electron	745:752	arg1	SEM					766:768	SEM	766:768	SEM	766:768	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	4	18	theme	electron	745:752	arg1	microscopy					754:763	scanning electron microscopy	736:763	scanning electron microscopy (SEM)	736:769	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	2	19	theme	fluoride	424:431	arg1	fluoride					424:431	fluoride	424:431	fluoride	424:431	The CS/PVA-La-Zr composite adsorbent can remove a large amount of fluoride within 1 min of contact time, and the adsorption equilibrium can be reached within 15 min.					
37268075	2	19	theme	fluoride	424:431	arg1	amount					414:419	a large amount	406:419	a large amount of fluoride within 1 min of contact time	406:460	The CS/PVA-La-Zr composite adsorbent can remove a large amount of fluoride within 1 min of contact time, and the adsorption equilibrium can be reached within 15 min.					
37268075	3	20	theme	adsorption	537:546	arg1	behavior					548:555	The fluoride adsorption behavior	524:555	The fluoride adsorption behavior of the CS/PVA-La-Zr composite	524:585	The fluoride adsorption behavior of the CS/PVA-La-Zr composite can be described by pseudo-second-order kinetics and Langmuir isotherms models.					
37268075	5	21	theme	ion	994:996	arg1	exchange					998:1005	ion exchange	994:1005	ion exchange	994:1005	The adsorption mechanism was studied using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), and which showed that ion exchange occurred mainly with hydroxide and fluoride ions.					
37268075	1	22	theme	fluoride	198:205	arg1	removal					187:193	the rapid removal	177:193	the rapid removal of fluoride from water	177:216	A novel and easily separable adsorbent in the shape of a membrane for the rapid removal of fluoride from water was prepared after testing Zr, La and LaZr to modify a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr).					
37268075	0	23	theme	alcohol	48:54	arg1	composite					56:64	Zirconium/lanthanum-modified chitosan/polyvinyl alcohol composite	0:64	Zirconium/lanthanum-modified chitosan/polyvinyl alcohol composite	0:64	Zirconium/lanthanum-modified chitosan/polyvinyl alcohol composite adsorbent for rapid removal of fluoride.					
37268075	5	24	theme	X-ray	932:936	arg1	XPS					966:968	XPS	966:968	XPS	966:968	The adsorption mechanism was studied using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), and which showed that ion exchange occurred mainly with hydroxide and fluoride ions.					
37268075	5	24	theme	X-ray	932:936	arg1	spectroscopy					952:963	X-ray photoelectron spectroscopy	932:963	X-ray photoelectron spectroscopy (XPS)	932:969	The adsorption mechanism was studied using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), and which showed that ion exchange occurred mainly with hydroxide and fluoride ions.					
37268075	3	25	theme	isotherms	649:657	arg1	models					659:664	Langmuir isotherms models	640:664	Langmuir isotherms models	640:664	The fluoride adsorption behavior of the CS/PVA-La-Zr composite can be described by pseudo-second-order kinetics and Langmuir isotherms models.					
37268075	5	26	dep	Fourier	881:887	arg1	transform					889:897	transform	889:897	transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS)	889:969	The adsorption mechanism was studied using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), and which showed that ion exchange occurred mainly with hydroxide and fluoride ions.					
37268075	2	27	theme	large	408:412	arg1	fluoride					424:431	fluoride	424:431	fluoride	424:431	The CS/PVA-La-Zr composite adsorbent can remove a large amount of fluoride within 1 min of contact time, and the adsorption equilibrium can be reached within 15 min.					
37268075	2	27	theme	large	408:412	arg1	amount					414:419	a large amount	406:419	a large amount of fluoride within 1 min of contact time	406:460	The CS/PVA-La-Zr composite adsorbent can remove a large amount of fluoride within 1 min of contact time, and the adsorption equilibrium can be reached within 15 min.					
37268075	1	28	theme	chitosan/polyvinyl	273:290	arg1	composite					300:308	a chitosan/polyvinyl alcohol composite adsorbent	271:318	a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr)	271:355	A novel and easily separable adsorbent in the shape of a membrane for the rapid removal of fluoride from water was prepared after testing Zr, La and LaZr to modify a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr).					
37268075	4	29	theme	X-ray	813:817	arg1	XRD					832:834	XRD	832:834	XRD	832:834	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	4	29	theme	X-ray	813:817	arg1	diffraction					819:829	X-ray diffraction	813:829	X-ray diffraction (XRD)	813:835	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	2	30	theme	time	457:460	arg1	1 min					440:444	1 min	440:444	1 min of contact time	440:460	The CS/PVA-La-Zr composite adsorbent can remove a large amount of fluoride within 1 min of contact time, and the adsorption equilibrium can be reached within 15 min.					
37268075	1	31	theme	alcohol	292:298	arg1	composite					300:308	a chitosan/polyvinyl alcohol composite adsorbent	271:318	a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr)	271:355	A novel and easily separable adsorbent in the shape of a membrane for the rapid removal of fluoride from water was prepared after testing Zr, La and LaZr to modify a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr).					
37268075	1	32	from	water	212:216	arg1	removal					187:193	the rapid removal	177:193	the rapid removal of fluoride from water	177:216	A novel and easily separable adsorbent in the shape of a membrane for the rapid removal of fluoride from water was prepared after testing Zr, La and LaZr to modify a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr).					
37268075	2	33	theme	contact	449:455	arg1	time					457:460	contact time	449:460	contact time	449:460	The CS/PVA-La-Zr composite adsorbent can remove a large amount of fluoride within 1 min of contact time, and the adsorption equilibrium can be reached within 15 min.					
37268075	3	34	theme	Langmuir	640:647	arg1	models					659:664	Langmuir isotherms models	640:664	Langmuir isotherms models	640:664	The fluoride adsorption behavior of the CS/PVA-La-Zr composite can be described by pseudo-second-order kinetics and Langmuir isotherms models.					
37268075	3	35	theme	CS/PVA-La-Zr	564:575	arg1	composite					577:585	the CS/PVA-La-Zr composite	560:585	the CS/PVA-La-Zr composite	560:585	The fluoride adsorption behavior of the CS/PVA-La-Zr composite can be described by pseudo-second-order kinetics and Langmuir isotherms models.					
37268075	0	36	theme	rapid	80:84	arg1	removal					86:92	rapid removal	80:92	rapid removal of fluoride	80:104	Zirconium/lanthanum-modified chitosan/polyvinyl alcohol composite adsorbent for rapid removal of fluoride.					
37268075	1	37	theme	adsorbent	310:318	arg1	composite					300:308	a chitosan/polyvinyl alcohol composite adsorbent	271:318	a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr)	271:355	A novel and easily separable adsorbent in the shape of a membrane for the rapid removal of fluoride from water was prepared after testing Zr, La and LaZr to modify a chitosan/polyvinyl alcohol composite adsorbent (CS/PVA-Zr, CS/PVA-La, CS/PVA-LA-Zr).					
37268075	3	38	theme	composite	577:585	arg1	behavior					548:555	The fluoride adsorption behavior	524:555	The fluoride adsorption behavior of the CS/PVA-La-Zr composite	524:585	The fluoride adsorption behavior of the CS/PVA-La-Zr composite can be described by pseudo-second-order kinetics and Langmuir isotherms models.					
37268075	6	39	theme	low-cost	1100:1107	arg1	CS/PVA-La-Zr					1138:1149	an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr	1080:1149	an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr	1080:1149	This study showed that an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr has the potential to remove fluoride effectively from drinking water in a short time.					
37268075	3	40	theme	pseudo-second-order	607:625	arg1	kinetics					627:634	pseudo-second-order kinetics	607:634	pseudo-second-order kinetics	607:634	The fluoride adsorption behavior of the CS/PVA-La-Zr composite can be described by pseudo-second-order kinetics and Langmuir isotherms models.					
37268075	4	41	theme	scanning	736:743	arg1	SEM					766:768	SEM	766:768	SEM	766:768	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	4	41	theme	scanning	736:743	arg1	microscopy					754:763	scanning electron microscopy	736:763	scanning electron microscopy (SEM)	736:769	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	5	42	theme	photoelectron	938:950	arg1	XPS					966:968	XPS	966:968	XPS	966:968	The adsorption mechanism was studied using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), and which showed that ion exchange occurred mainly with hydroxide and fluoride ions.					
37268075	5	42	theme	photoelectron	938:950	arg1	spectroscopy					952:963	X-ray photoelectron spectroscopy	932:963	X-ray photoelectron spectroscopy (XPS)	932:969	The adsorption mechanism was studied using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), and which showed that ion exchange occurred mainly with hydroxide and fluoride ions.					
37268075	4	43	dep	morphology	671:680	arg1	The					667:669	The	667:669	The	667:669	The morphology and structure of the adsorbents were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD).					
37268075	6	44	theme	operable	1090:1097	arg1	CS/PVA-La-Zr					1138:1149	an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr	1080:1149	an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr	1080:1149	This study showed that an easily operable, low-cost and environmentally friendly CS/PVA-La-Zr has the potential to remove fluoride effectively from drinking water in a short time.					
37268075	0	45	theme	fluoride	97:104	arg1	removal					86:92	rapid removal	80:92	rapid removal of fluoride	80:104	Zirconium/lanthanum-modified chitosan/polyvinyl alcohol composite adsorbent for rapid removal of fluoride.					
37268075	2	46	theme	adsorption	471:480	arg1	equilibrium					482:492	the adsorption equilibrium	467:492	the adsorption equilibrium	467:492	The CS/PVA-La-Zr composite adsorbent can remove a large amount of fluoride within 1 min of contact time, and the adsorption equilibrium can be reached within 15 min.					
36281730	6	0	theme	cocktail	924:931	arg1	treatment					933:941	the antibiotic cocktail treatment	909:941	the antibiotic cocktail treatment	909:941	In addition, salecan treatment modulated the composition of the gut microbiota, and the antibiotic cocktail treatment indicated that the hepatoprotective effect of salecan was dependent on the gut microbiota.					
36281730	5	1	theme	treatment	708:716	arg1	weeks					699:703	8 weeks	697:703	8 weeks of treatment	697:716	After 8 weeks of treatment, salecan alleviated liver injury by regulating oxidative stress and activating the Nrf2 signaling pathway.					
36281730	8	2	theme	microbiota-derived	1199:1216	arg1	metabolites					1218:1228	microbiota-derived metabolites	1199:1228	microbiota-derived metabolites of salecan	1199:1239	Besides, microbiota-derived metabolites of salecan also contributed to the hepatoprotective effect of salecan against liver injury and inhibited oxidative stress.					
36281730	3	3	theme	gut	468:470	arg1	microbiota					472:481	the gut microbiota	464:481	the gut microbiota	464:481	β-Glucan can induce shifts in the gut microbiota and show health benefits; however, whether modulation of the gut microbiota by β-glucan is associated with its benefits remains unclear.					
36281730	1	4	theme	novel	198:202	arg1	ingredients					209:219	the novel food ingredients	194:219	the novel food ingredients approved in China	194:237	Salecan, a natural β-glucan consisting of seven residues linked by β-(1→3)/α-(1→3) glycosidic bonds, is one of the novel food ingredients approved in China.					
36281730	7	5	theme	liver	1176:1180	arg1	injury					1182:1187	liver injury	1176:1187	liver injury	1176:1187	Fecal microbiota transplantation was used to further verify this mechanism, and we confirmed that microbial colonization partially alleviated liver injury.					
36281730	6	6	theme	salecan	838:844	arg1	treatment					846:854	salecan treatment	838:854	salecan treatment	838:854	In addition, salecan treatment modulated the composition of the gut microbiota, and the antibiotic cocktail treatment indicated that the hepatoprotective effect of salecan was dependent on the gut microbiota.					
36281730	3	7	theme	microbiota	472:481	arg1	modulation					450:459	modulation	450:459	modulation of the gut microbiota by β-glucan	450:493	β-Glucan can induce shifts in the gut microbiota and show health benefits; however, whether modulation of the gut microbiota by β-glucan is associated with its benefits remains unclear.					
36281730	4	8	theme	liver	659:663	arg1	injury					665:670	CCl4-induced liver injury	646:670	CCl4-induced liver injury	646:670	Here, the hepatoprotective effect and potential mechanism of salecan supplementation using a model of CCl4-induced liver injury were investigated.					
36281730	4	9	theme	CCl4-induced	646:657	arg1	injury					665:670	CCl4-induced liver injury	646:670	CCl4-induced liver injury	646:670	Here, the hepatoprotective effect and potential mechanism of salecan supplementation using a model of CCl4-induced liver injury were investigated.					
36281730	4	10	theme	salecan	605:611	arg1	supplementation					613:627	salecan supplementation	605:627	salecan supplementation using a model of CCl4-induced liver injury	605:670	Here, the hepatoprotective effect and potential mechanism of salecan supplementation using a model of CCl4-induced liver injury were investigated.					
36281730	5	11	theme	Nrf2	801:804	arg1	pathway					816:822	the Nrf2 signaling pathway	797:822	the Nrf2 signaling pathway	797:822	After 8 weeks of treatment, salecan alleviated liver injury by regulating oxidative stress and activating the Nrf2 signaling pathway.					
36281730	1	12	theme	food	204:207	arg1	ingredients					209:219	the novel food ingredients	194:219	the novel food ingredients approved in China	194:237	Salecan, a natural β-glucan consisting of seven residues linked by β-(1→3)/α-(1→3) glycosidic bonds, is one of the novel food ingredients approved in China.					
36281730	1	13	from	China	233:237	arg1	approved					221:228	approved	221:228	approved	221:228	Salecan, a natural β-glucan consisting of seven residues linked by β-(1→3)/α-(1→3) glycosidic bonds, is one of the novel food ingredients approved in China.					
36281730	4	14	theme	potential	582:590	arg1	mechanism					592:600	potential mechanism	582:600	potential mechanism	582:600	Here, the hepatoprotective effect and potential mechanism of salecan supplementation using a model of CCl4-induced liver injury were investigated.					
36281730	8	15	theme	salecan	1292:1298	arg1	effect					1282:1287	the hepatoprotective effect	1261:1287	the hepatoprotective effect of salecan against liver injury	1261:1319	Besides, microbiota-derived metabolites of salecan also contributed to the hepatoprotective effect of salecan against liver injury and inhibited oxidative stress.					
36281730	6	16	theme	salecan	989:995	arg1	dependent					1001:1009	dependent	1001:1009	dependent	1001:1009	In addition, salecan treatment modulated the composition of the gut microbiota, and the antibiotic cocktail treatment indicated that the hepatoprotective effect of salecan was dependent on the gut microbiota.					
36281730	6	16	theme	salecan	989:995	arg1	effect					979:984	the hepatoprotective effect	958:984	the hepatoprotective effect of salecan	958:995	In addition, salecan treatment modulated the composition of the gut microbiota, and the antibiotic cocktail treatment indicated that the hepatoprotective effect of salecan was dependent on the gut microbiota.					
36281730	7	17	theme	Fecal	1034:1038	arg1	transplantation					1051:1065	Fecal microbiota transplantation	1034:1065	Fecal microbiota transplantation	1034:1065	Fecal microbiota transplantation was used to further verify this mechanism, and we confirmed that microbial colonization partially alleviated liver injury.					
36281730	6	18	theme	antibiotic	913:922	arg1	treatment					933:941	the antibiotic cocktail treatment	909:941	the antibiotic cocktail treatment	909:941	In addition, salecan treatment modulated the composition of the gut microbiota, and the antibiotic cocktail treatment indicated that the hepatoprotective effect of salecan was dependent on the gut microbiota.					
36281730	2	19	theme	liver	318:322	arg1	injury					324:329	liver injury	318:329	liver injury	318:329	β-Glucan has a variety of health-improving effects, yet its mechanism against liver injury remains poorly understood.					
36281730	7	20	theme	microbial	1132:1140	arg1	colonization					1142:1153	microbial colonization	1132:1153	microbial colonization	1132:1153	Fecal microbiota transplantation was used to further verify this mechanism, and we confirmed that microbial colonization partially alleviated liver injury.					
36281730	1	21	theme	ingredients	209:219	arg1	ingredients					209:219	the novel food ingredients	194:219	the novel food ingredients approved in China	194:237	Salecan, a natural β-glucan consisting of seven residues linked by β-(1→3)/α-(1→3) glycosidic bonds, is one of the novel food ingredients approved in China.					
36281730	1	21	theme	ingredients	209:219	arg1	one					187:189	one	187:189	one	187:189	Salecan, a natural β-glucan consisting of seven residues linked by β-(1→3)/α-(1→3) glycosidic bonds, is one of the novel food ingredients approved in China.					
36281730	1	22	from	approved	221:228	arg1	China					233:237	China	233:237	China	233:237	Salecan, a natural β-glucan consisting of seven residues linked by β-(1→3)/α-(1→3) glycosidic bonds, is one of the novel food ingredients approved in China.					
36281730	1	23	theme	approved	221:228	arg1	ingredients					209:219	the novel food ingredients	194:219	the novel food ingredients approved in China	194:237	Salecan, a natural β-glucan consisting of seven residues linked by β-(1→3)/α-(1→3) glycosidic bonds, is one of the novel food ingredients approved in China.					
36281730	9	24	theme	gut	1446:1448	arg1	microbiota					1450:1459	the gut microbiota	1442:1459	the gut microbiota	1442:1459	These findings supported that salecan intervention attenuated liver injury by regulating the gut microbiota and its metabolites.					
36281730	4	25	theme	hepatoprotective	554:569	arg1	effect					571:576	the hepatoprotective effect	550:576	the hepatoprotective effect	550:576	Here, the hepatoprotective effect and potential mechanism of salecan supplementation using a model of CCl4-induced liver injury were investigated.					
36281730	0	26	theme	liver	20:24	arg1	injury					26:31	liver injury	20:31	liver injury	20:31	Salecan ameliorates liver injury by regulating gut microbiota and its metabolites.					
36281730	1	27	theme	natural	94:100	arg1	β-glucan					102:109	a natural β-glucan	92:109	a natural β-glucan consisting of seven residues linked by β-(1→3)/α-(1→3) glycosidic bonds	92:181	Salecan, a natural β-glucan consisting of seven residues linked by β-(1→3)/α-(1→3) glycosidic bonds, is one of the novel food ingredients approved in China.					
36281730	1	27	theme	natural	94:100	arg1	Salecan					83:89	Salecan	83:89	Salecan	83:89	Salecan, a natural β-glucan consisting of seven residues linked by β-(1→3)/α-(1→3) glycosidic bonds, is one of the novel food ingredients approved in China.					
36281730	8	28	theme	hepatoprotective	1265:1280	arg1	effect					1282:1287	the hepatoprotective effect	1261:1287	the hepatoprotective effect of salecan against liver injury	1261:1319	Besides, microbiota-derived metabolites of salecan also contributed to the hepatoprotective effect of salecan against liver injury and inhibited oxidative stress.					
36281730	6	29	theme	gut	1018:1020	arg1	microbiota					1022:1031	the gut microbiota	1014:1031	the gut microbiota	1014:1031	In addition, salecan treatment modulated the composition of the gut microbiota, and the antibiotic cocktail treatment indicated that the hepatoprotective effect of salecan was dependent on the gut microbiota.					
36281730	6	30	theme	microbiota	893:902	arg1	composition					870:880	the composition	866:880	the composition of the gut microbiota	866:902	In addition, salecan treatment modulated the composition of the gut microbiota, and the antibiotic cocktail treatment indicated that the hepatoprotective effect of salecan was dependent on the gut microbiota.					
36281730	1	31	theme	glycosidic	166:175	arg1	bonds					177:181	β-(1→3)/α-(1→3) glycosidic bonds	150:181	β-(1→3)/α-(1→3) glycosidic bonds	150:181	Salecan, a natural β-glucan consisting of seven residues linked by β-(1→3)/α-(1→3) glycosidic bonds, is one of the novel food ingredients approved in China.					
36281730	6	32	theme	gut	889:891	arg1	microbiota					893:902	the gut microbiota	885:902	the gut microbiota	885:902	In addition, salecan treatment modulated the composition of the gut microbiota, and the antibiotic cocktail treatment indicated that the hepatoprotective effect of salecan was dependent on the gut microbiota.					
36281730	5	33	theme	liver	738:742	arg1	injury					744:749	liver injury	738:749	liver injury	738:749	After 8 weeks of treatment, salecan alleviated liver injury by regulating oxidative stress and activating the Nrf2 signaling pathway.					
36281730	8	34	link	microbiota-derived	1199:1216	arg1	metabolites					1218:1228	microbiota-derived metabolites	1199:1228	microbiota-derived metabolites of salecan	1199:1239	Besides, microbiota-derived metabolites of salecan also contributed to the hepatoprotective effect of salecan against liver injury and inhibited oxidative stress.					
36281730	2	35	theme	effects	283:289	arg1	variety					255:261	a variety	253:261	a variety of health-improving effects	253:289	β-Glucan has a variety of health-improving effects, yet its mechanism against liver injury remains poorly understood.					
36281730	2	35	theme	effects	283:289	arg1	effects					283:289	health-improving effects	266:289	health-improving effects	266:289	β-Glucan has a variety of health-improving effects, yet its mechanism against liver injury remains poorly understood.					
36281730	0	36	theme	gut	47:49	arg1	microbiota					51:60	gut microbiota	47:60	gut microbiota	47:60	Salecan ameliorates liver injury by regulating gut microbiota and its metabolites.					
36281730	2	37	contain	has	249:251	arg1	β-Glucan					240:247	β-Glucan	240:247	β-Glucan	240:247	β-Glucan has a variety of health-improving effects, yet its mechanism against liver injury remains poorly understood.					
36281730	2	37	contain	has	249:251	arg2	effects					283:289	health-improving effects	266:289	health-improving effects	266:289	β-Glucan has a variety of health-improving effects, yet its mechanism against liver injury remains poorly understood.					
36281730	2	37	contain	has	249:251	arg2	variety					255:261	a variety	253:261	a variety of health-improving effects	253:289	β-Glucan has a variety of health-improving effects, yet its mechanism against liver injury remains poorly understood.					
36281730	2	38	theme	health-improving	266:281	arg1	effects					283:289	health-improving effects	266:289	health-improving effects	266:289	β-Glucan has a variety of health-improving effects, yet its mechanism against liver injury remains poorly understood.					
36281730	8	39	theme	salecan	1233:1239	arg1	metabolites					1218:1228	microbiota-derived metabolites	1199:1228	microbiota-derived metabolites of salecan	1199:1239	Besides, microbiota-derived metabolites of salecan also contributed to the hepatoprotective effect of salecan against liver injury and inhibited oxidative stress.					
36281730	4	40	theme	injury	665:670	arg1	model					637:641	a model	635:641	a model of CCl4-induced liver injury	635:670	Here, the hepatoprotective effect and potential mechanism of salecan supplementation using a model of CCl4-induced liver injury were investigated.					
36281730	9	41	theme	salecan	1383:1389	arg1	intervention					1391:1402	salecan intervention	1383:1402	salecan intervention	1383:1402	These findings supported that salecan intervention attenuated liver injury by regulating the gut microbiota and its metabolites.					
36281730	8	42	theme	oxidative	1335:1343	arg1	stress					1345:1350	oxidative stress	1335:1350	oxidative stress	1335:1350	Besides, microbiota-derived metabolites of salecan also contributed to the hepatoprotective effect of salecan against liver injury and inhibited oxidative stress.					
36281730	3	43	theme	health	416:421	arg1	benefits					423:430	health benefits	416:430	health benefits	416:430	β-Glucan can induce shifts in the gut microbiota and show health benefits; however, whether modulation of the gut microbiota by β-glucan is associated with its benefits remains unclear.					
36281730	7	44	theme	microbiota	1040:1049	arg1	transplantation					1051:1065	Fecal microbiota transplantation	1034:1065	Fecal microbiota transplantation	1034:1065	Fecal microbiota transplantation was used to further verify this mechanism, and we confirmed that microbial colonization partially alleviated liver injury.					
36281730	3	45	theme	gut	392:394	arg1	microbiota					396:405	the gut microbiota	388:405	the gut microbiota	388:405	β-Glucan can induce shifts in the gut microbiota and show health benefits; however, whether modulation of the gut microbiota by β-glucan is associated with its benefits remains unclear.					
36281730	8	46	theme	liver	1308:1312	arg1	injury					1314:1319	liver injury	1308:1319	liver injury	1308:1319	Besides, microbiota-derived metabolites of salecan also contributed to the hepatoprotective effect of salecan against liver injury and inhibited oxidative stress.					
36281730	5	47	theme	signaling	806:814	arg1	pathway					816:822	the Nrf2 signaling pathway	797:822	the Nrf2 signaling pathway	797:822	After 8 weeks of treatment, salecan alleviated liver injury by regulating oxidative stress and activating the Nrf2 signaling pathway.					
36281730	6	48	theme	hepatoprotective	962:977	arg1	dependent					1001:1009	dependent	1001:1009	dependent	1001:1009	In addition, salecan treatment modulated the composition of the gut microbiota, and the antibiotic cocktail treatment indicated that the hepatoprotective effect of salecan was dependent on the gut microbiota.					
36281730	6	48	theme	hepatoprotective	962:977	arg1	effect					979:984	the hepatoprotective effect	958:984	the hepatoprotective effect of salecan	958:995	In addition, salecan treatment modulated the composition of the gut microbiota, and the antibiotic cocktail treatment indicated that the hepatoprotective effect of salecan was dependent on the gut microbiota.					
36281730	7	49	used	used	1071:1074	arg2	transplantation					1051:1065	Fecal microbiota transplantation	1034:1065	Fecal microbiota transplantation	1034:1065	Fecal microbiota transplantation was used to further verify this mechanism, and we confirmed that microbial colonization partially alleviated liver injury.					
36281730	5	50	theme	oxidative	765:773	arg1	stress					775:780	oxidative stress	765:780	oxidative stress	765:780	After 8 weeks of treatment, salecan alleviated liver injury by regulating oxidative stress and activating the Nrf2 signaling pathway.					
36281730	3	51	from	shifts	378:383	arg1	microbiota					396:405	the gut microbiota	388:405	the gut microbiota	388:405	β-Glucan can induce shifts in the gut microbiota and show health benefits; however, whether modulation of the gut microbiota by β-glucan is associated with its benefits remains unclear.					
36281730	4	52	theme	supplementation	613:627	arg1	effect					571:576	the hepatoprotective effect	550:576	the hepatoprotective effect	550:576	Here, the hepatoprotective effect and potential mechanism of salecan supplementation using a model of CCl4-induced liver injury were investigated.					
36281730	4	52	theme	supplementation	613:627	arg1	mechanism					592:600	potential mechanism	582:600	potential mechanism	582:600	Here, the hepatoprotective effect and potential mechanism of salecan supplementation using a model of CCl4-induced liver injury were investigated.					
36281730	9	53	theme	liver	1415:1419	arg1	injury					1421:1426	liver injury	1415:1426	liver injury	1415:1426	These findings supported that salecan intervention attenuated liver injury by regulating the gut microbiota and its metabolites.					
35835087	0	0	theme	rapid	95:99	arg1	healing					101:107	rapid healing	95:107	rapid healing	95:107	Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect.					
35835087	1	1	dep	epidermis	296:304	arg1	layers					317:322	layers	317:322	layers	317:322	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	1	1	dep	epidermis	296:304	arg1	the					292:294	the	292:294	the	292:294	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	6	2	theme	cells	1098:1102	arg1	area					1042:1045	The migratory area	1028:1045	The migratory area of Cu-doped group human umbilical vein endothelial cells	1028:1102	The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment.					
35835087	2	3	theme	wound	478:482	arg1	dressing					484:491	a bio-inspired double-layer asymmetric wettable wound dressing	430:491	a bio-inspired double-layer asymmetric wettable wound dressing	430:491	Herein, a bio-inspired double-layer asymmetric wettable wound dressing was prepared by low-temperature 3D printing coupled with electrospinning technology.					
35835087	1	4	dep	characteristics	259:273	arg1	the					255:257	the	255:257	the	255:257	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	1	5	used	used	241:244	arg2	dressings					148:156	Asymmetrical dressings	135:156	Asymmetrical dressings	135:156	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	7	6	theme	CS5-PCL	1298:1304	arg1	dressing					1306:1313	the 0.5Cu@CS5-PCL dressing	1288:1313	the 0.5Cu@CS5-PCL dressing	1288:1313	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	5	7	theme	propertiesin	997:1008	arg1	vitroexperiments					1010:1025	antibacterial propertiesin vitroexperiments	983:1025	antibacterial propertiesin vitroexperiments	983:1025	Additionally, the composite dressings also showed excellent cytocompatibility and antibacterial propertiesin vitroexperiments.					
35835087	6	8	theme	scratch	1200:1206	arg1	experiment					1208:1217	the cell scratch experiment	1191:1217	the cell scratch experiment	1191:1217	The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment.					
35835087	4	9	theme	dressing	762:769	arg1	layer					749:753	The hydrophilic bottom layer	726:753	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap)	726:845	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	7	10	theme	enormous	1360:1367	arg1	potential					1369:1377	the enormous potential	1356:1377	the enormous potential of asymmetric double dressings in boosting wound healing	1356:1434	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	2	11	theme	3D	525:526	arg1	printing					528:535	low-temperature 3D printing	509:535	low-temperature 3D printing coupled with electrospinning technology	509:575	Herein, a bio-inspired double-layer asymmetric wettable wound dressing was prepared by low-temperature 3D printing coupled with electrospinning technology.					
35835087	2	12	theme	low-temperature	509:523	arg1	printing					528:535	low-temperature 3D printing	509:535	low-temperature 3D printing coupled with electrospinning technology	509:575	Herein, a bio-inspired double-layer asymmetric wettable wound dressing was prepared by low-temperature 3D printing coupled with electrospinning technology.					
35835087	4	13	theme	bottom	742:747	arg1	layer					749:753	The hydrophilic bottom layer	726:753	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap)	726:845	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	7	14	theme	asymmetric	1382:1391	arg1	dressings					1400:1408	asymmetric double dressings	1382:1408	asymmetric double dressings	1382:1408	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	6	15	theme	human	1065:1069	arg1	cells					1098:1102	Cu-doped group human umbilical vein endothelial cells	1050:1102	Cu-doped group human umbilical vein endothelial cells	1050:1102	The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment.					
35835087	4	16	dep	chitosan	795:802	arg1	Laponite					826:833	doped Laponite	820:833	doped Laponite (Cu@CS-Lap)	820:845	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	4	16	dep	chitosan	795:802	arg1	CS-Lap					839:844	Cu@CS-Lap	836:844	Cu@CS-Lap	836:844	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	0	17	theme	antibacterial	113:125	arg1	effect					127:132	antibacterial effect	113:132	antibacterial effect	113:132	Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect.					
35835087	3	18	theme	air-permeable	701:713	arg1	epidermis					715:723	the compact and air-permeable epidermis	685:723	the compact and air-permeable epidermis	685:723	The hydrophobic top layer of poly(caprolactone)(PCL) film produced by electrospinning was used to simulate the compact and air-permeable epidermis.					
35835087	2	19	theme	wettable	469:476	arg1	dressing					484:491	a bio-inspired double-layer asymmetric wettable wound dressing	430:491	a bio-inspired double-layer asymmetric wettable wound dressing	430:491	Herein, a bio-inspired double-layer asymmetric wettable wound dressing was prepared by low-temperature 3D printing coupled with electrospinning technology.					
35835087	7	20	theme	double	1393:1398	arg1	dressings					1400:1408	asymmetric double dressings	1382:1408	asymmetric double dressings	1382:1408	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	7	21	theme	wound	1422:1426	arg1	healing					1428:1434	boosting wound healing	1413:1434	boosting wound healing	1413:1434	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	1	22	theme	compact	183:189	arg1	layer					195:199	a compact top layer	181:199	a compact top layer	181:199	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	4	23	theme	wound	886:890	arg1	healing					892:898	wound healing	886:898	wound healing	886:898	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	1	24	theme	traditional	351:361	arg1	dressings					363:371	traditional dressings	351:371	traditional dressings such as wound dryness and bacterial penetration	351:419	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	1	24	theme	traditional	351:361	arg1	dryness					387:393	wound dryness	381:393	wound dryness	381:393	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	1	24	theme	traditional	351:361	arg1	penetration					409:419	bacterial penetration	399:419	bacterial penetration	399:419	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	4	25	theme	@	838:838	arg1	Laponite					826:833	doped Laponite	820:833	doped Laponite (Cu@CS-Lap)	820:845	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	4	25	theme	@	838:838	arg1	CS-Lap					839:844	Cu@CS-Lap	836:844	Cu@CS-Lap	836:844	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	7	26	from	dressing	1306:1313	arg1	closure					1277:1283	wound closure	1271:1283	wound closure at the 0.5Cu@CS5-PCL dressing	1271:1313	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	3	27	theme	hydrophobic	582:592	arg1	layer					598:602	The hydrophobic top layer	578:602	The hydrophobic top layer of poly(caprolactone)(PCL) film produced by electrospinning	578:662	The hydrophobic top layer of poly(caprolactone)(PCL) film produced by electrospinning was used to simulate the compact and air-permeable epidermis.					
35835087	1	28	theme	top	191:193	arg1	layer					195:199	a compact top layer	181:199	a compact top layer	181:199	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	7	29	theme	dressings	1400:1408	arg1	potential					1369:1377	the enormous potential	1356:1377	the enormous potential of asymmetric double dressings in boosting wound healing	1356:1434	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	4	30	theme	Cu	836:837	arg1	Laponite					826:833	doped Laponite	820:833	doped Laponite (Cu@CS-Lap)	820:845	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	4	30	theme	Cu	836:837	arg1	CS-Lap					839:844	Cu@CS-Lap	836:844	Cu@CS-Lap	836:844	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	3	31	theme	top	594:596	arg1	layer					598:602	The hydrophobic top layer	578:602	The hydrophobic top layer of poly(caprolactone)(PCL) film produced by electrospinning	578:662	The hydrophobic top layer of poly(caprolactone)(PCL) film produced by electrospinning was used to simulate the compact and air-permeable epidermis.					
35835087	6	32	theme	group	1059:1063	arg1	cells					1098:1102	Cu-doped group human umbilical vein endothelial cells	1050:1102	Cu-doped group human umbilical vein endothelial cells	1050:1102	The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment.					
35835087	0	33	theme	bionic	17:22	arg1	dressing					81:88	a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing	15:88	a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing	15:88	Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect.					
35835087	4	34	used	used	851:854	arg2	layer					749:753	The hydrophilic bottom layer	726:753	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap)	726:845	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	7	35	from	vivoexperiments	1235:1249	arg1	rats					1254:1257	rats	1254:1257	rats	1254:1257	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	4	36	theme	copper	808:813	arg1	ions					815:818	copper ions	808:818	copper ions	808:818	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	6	37	theme	Cu-doped	1050:1057	arg1	cells					1098:1102	Cu-doped group human umbilical vein endothelial cells	1050:1102	Cu-doped group human umbilical vein endothelial cells	1050:1102	The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment.					
35835087	4	38	theme	hydrophilic	730:740	arg1	layer					749:753	The hydrophilic bottom layer	726:753	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap)	726:845	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	2	39	theme	electrospinning	550:564	arg1	technology					566:575	electrospinning technology	550:575	electrospinning technology	550:575	Herein, a bio-inspired double-layer asymmetric wettable wound dressing was prepared by low-temperature 3D printing coupled with electrospinning technology.					
35835087	1	40	theme	dressings	363:371	arg1	flaws					342:346	the flaws	338:346	the flaws of traditional dressings such as wound dryness and bacterial penetration	338:419	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	6	41	theme	endothelial	1086:1096	arg1	cells					1098:1102	Cu-doped group human umbilical vein endothelial cells	1050:1102	Cu-doped group human umbilical vein endothelial cells	1050:1102	The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment.					
35835087	2	42	theme	double-layer	445:456	arg1	dressing					484:491	a bio-inspired double-layer asymmetric wettable wound dressing	430:491	a bio-inspired double-layer asymmetric wettable wound dressing	430:491	Herein, a bio-inspired double-layer asymmetric wettable wound dressing was prepared by low-temperature 3D printing coupled with electrospinning technology.					
35835087	6	43	theme	control	1146:1152	arg1	group					1154:1158	the control group	1142:1158	the control group	1142:1158	The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment.					
35835087	0	44	theme	wettable	35:42	arg1	dressing					81:88	a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing	15:88	a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing	15:88	Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect.					
35835087	7	45	theme	wound	1271:1275	arg1	closure					1277:1283	wound closure	1271:1283	wound closure at the 0.5Cu@CS5-PCL dressing	1271:1313	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	3	46	theme	poly	607:610	arg1	film					631:634	poly(caprolactone)(PCL) film	607:634	poly(caprolactone)(PCL) film produced by electrospinning	607:662	The hydrophobic top layer of poly(caprolactone)(PCL) film produced by electrospinning was used to simulate the compact and air-permeable epidermis.					
35835087	3	47	used	used	668:671	arg2	layer					598:602	The hydrophobic top layer	578:602	The hydrophobic top layer of poly(caprolactone)(PCL) film produced by electrospinning	578:662	The hydrophobic top layer of poly(caprolactone)(PCL) film produced by electrospinning was used to simulate the compact and air-permeable epidermis.					
35835087	1	48	theme	porous	207:212	arg1	layer					221:225	a porous bottom layer	205:225	a porous bottom layer	205:225	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	0	49	theme	asymmetric	24:33	arg1	dressing					81:88	a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing	15:88	a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing	15:88	Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect.					
35835087	2	50	theme	asymmetric	458:467	arg1	dressing					484:491	a bio-inspired double-layer asymmetric wettable wound dressing	430:491	a bio-inspired double-layer asymmetric wettable wound dressing	430:491	Herein, a bio-inspired double-layer asymmetric wettable wound dressing was prepared by low-temperature 3D printing coupled with electrospinning technology.					
35835087	6	51	theme	vein	1081:1084	arg1	cells					1098:1102	Cu-doped group human umbilical vein endothelial cells	1050:1102	Cu-doped group human umbilical vein endothelial cells	1050:1102	The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment.					
35835087	1	52	theme	bottom	214:219	arg1	layer					221:225	a porous bottom layer	205:225	a porous bottom layer	205:225	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	0	53	theme	chitosan-laponite-PCL	53:73	arg1	dressing					81:88	a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing	15:88	a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing	15:88	Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect.					
35835087	1	54	theme	epidermis	296:304	arg1	structure					279:287	structure	279:287	structure	279:287	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	1	54	theme	epidermis	296:304	arg1	characteristics					259:273	characteristics	259:273	characteristics	259:273	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	1	55	theme	wound	381:385	arg1	dryness					387:393	wound dryness	381:393	wound dryness	381:393	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	4	56	theme	doped	820:824	arg1	Laponite					826:833	doped Laponite	820:833	doped Laponite (Cu@CS-Lap)	820:845	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	4	56	theme	doped	820:824	arg1	CS-Lap					839:844	Cu@CS-Lap	836:844	Cu@CS-Lap	836:844	The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing.					
35835087	7	57	theme	boosting	1413:1420	arg1	healing					1428:1434	boosting wound healing	1413:1434	boosting wound healing	1413:1434	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	0	58	theme	Cu-doped	44:51	arg1	dressing					81:88	a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing	15:88	a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing	15:88	Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect.					
35835087	6	59	theme	experiment	1208:1217	arg1	results					1180:1186	the results	1176:1186	the results of the cell scratch experiment	1176:1217	The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment.					
35835087	7	60	theme	@	1297:1297	arg1	dressing					1306:1313	the 0.5Cu@CS5-PCL dressing	1288:1313	the 0.5Cu@CS5-PCL dressing	1288:1313	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	2	61	theme	bio-inspired	432:443	arg1	dressing					484:491	a bio-inspired double-layer asymmetric wettable wound dressing	430:491	a bio-inspired double-layer asymmetric wettable wound dressing	430:491	Herein, a bio-inspired double-layer asymmetric wettable wound dressing was prepared by low-temperature 3D printing coupled with electrospinning technology.					
35835087	6	62	theme	cell	1195:1198	arg1	experiment					1208:1217	the cell scratch experiment	1191:1217	the cell scratch experiment	1191:1217	The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment.					
35835087	1	63	theme	Asymmetrical	135:146	arg1	dressings					148:156	Asymmetrical dressings	135:156	Asymmetrical dressings	135:156	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	1	64	theme	dermis	310:315	arg1	structure					279:287	structure	279:287	structure	279:287	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	1	64	theme	dermis	310:315	arg1	characteristics					259:273	characteristics	259:273	characteristics	259:273	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	5	65	theme	excellent	951:959	arg1	cytocompatibility					961:977	excellent cytocompatibility	951:977	excellent cytocompatibility	951:977	Additionally, the composite dressings also showed excellent cytocompatibility and antibacterial propertiesin vitroexperiments.					
35835087	0	66	with	Fabrication	0:10	arg1	healing					101:107	rapid healing	95:107	rapid healing	95:107	Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect.					
35835087	0	66	with	Fabrication	0:10	arg1	effect					127:132	antibacterial effect	113:132	antibacterial effect	113:132	Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect.					
35835087	3	67	theme	film	631:634	arg1	layer					598:602	The hydrophobic top layer	578:602	The hydrophobic top layer of poly(caprolactone)(PCL) film produced by electrospinning	578:662	The hydrophobic top layer of poly(caprolactone)(PCL) film produced by electrospinning was used to simulate the compact and air-permeable epidermis.					
35835087	7	68	from	potential	1369:1377	arg1	healing					1428:1434	boosting wound healing	1413:1434	boosting wound healing	1413:1434	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	1	69	theme	bacterial	399:407	arg1	penetration					409:419	bacterial penetration	399:419	bacterial penetration	399:419	Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration.					
35835087	5	70	theme	composite	919:927	arg1	dressings					929:937	the composite dressings	915:937	the composite dressings	915:937	Additionally, the composite dressings also showed excellent cytocompatibility and antibacterial propertiesin vitroexperiments.					
35835087	7	71	theme	0.5Cu	1292:1296	arg1	dressing					1306:1313	the 0.5Cu@CS5-PCL dressing	1288:1313	the 0.5Cu@CS5-PCL dressing	1288:1313	Furthermore,in vivoexperiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.					
35835087	0	72	theme	dressing	81:88	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect.	0:133	Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect.					
35835087	6	73	theme	migratory	1032:1040	arg1	area					1042:1045	The migratory area	1028:1045	The migratory area of Cu-doped group human umbilical vein endothelial cells	1028:1102	The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment.					
35835087	6	74	theme	umbilical	1071:1079	arg1	cells					1098:1102	Cu-doped group human umbilical vein endothelial cells	1050:1102	Cu-doped group human umbilical vein endothelial cells	1050:1102	The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment.					
35835087	0	75	theme	wound	75:79	arg1	dressing					81:88	a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing	15:88	a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing	15:88	Fabrication of a bionic asymmetric wettable Cu-doped chitosan-laponite-PCL wound dressing with rapid healing and antibacterial effect.					
35835087	3	76	theme	compact	689:695	arg1	epidermis					715:723	the compact and air-permeable epidermis	685:723	the compact and air-permeable epidermis	685:723	The hydrophobic top layer of poly(caprolactone)(PCL) film produced by electrospinning was used to simulate the compact and air-permeable epidermis.					
35835087	5	77	theme	antibacterial	983:995	arg1	vitroexperiments					1010:1025	antibacterial propertiesin vitroexperiments	983:1025	antibacterial propertiesin vitroexperiments	983:1025	Additionally, the composite dressings also showed excellent cytocompatibility and antibacterial propertiesin vitroexperiments.					
35917370	6	0	theme	dermal	984:989	arg1	repair					991:996	more uniform dermal repair	971:996	more uniform dermal repair	971:996	An acellular dermal substitute consisting of collagen and glycosaminoglycans (C-GAG) provided more uniform dermal repair, and reduced needs for epidermal harvesting but was subject to loss from microbial contamination.					
35917370	3	1	theme	wound	499:503	arg1	closure					505:511	wound closure	499:511	wound closure	499:511	Because STAG availability may be insufficient in life-threatening burns, alternatives have been evaluated for safety and efficacy of wound closure.					
35917370	10	2	theme	Current	1547:1553	arg1	alternatives					1555:1566	Current alternatives	1547:1566	Current alternatives	1547:1566	Current alternatives act by mechanisms of wound healing, not by developmental biology by which skin forms in utero with pigment, hair, sweat and sebaceous glands, microvasculature, and nerve.					
35917370	3	3	from	insufficient	399:410	arg1	burns					432:436	life-threatening burns	415:436	life-threatening burns	415:436	Because STAG availability may be insufficient in life-threatening burns, alternatives have been evaluated for safety and efficacy of wound closure.					
35917370	6	4	from	contamination	1081:1093	arg1	loss					1061:1064	loss	1061:1064	loss from microbial contamination	1061:1093	An acellular dermal substitute consisting of collagen and glycosaminoglycans (C-GAG) provided more uniform dermal repair, and reduced needs for epidermal harvesting but was subject to loss from microbial contamination.					
35917370	2	5	theme	extensive	343:351	arg1	burns					359:363	extensive, deep burns	343:363	extensive, deep burns	343:363	Historically, split-thickness skin autograft (STAG) has served as the prevailing standard of care for closure of extensive, deep burns.					
35917370	0	6	theme	Excised	100:106	arg1	Burns					124:128	Excised, Full-Thickness Burns	100:128	Excised, Full-Thickness Burns	100:128	Composition and Performance of Autologous Engineered Skin Substitutes for Repair or Regeneration of Excised, Full-Thickness Burns.					
35917370	9	7	theme	uninjured	1531:1539	arg1	skin					1541:1544	uninjured skin	1531:1544	uninjured skin	1531:1544	Despite these advances, no current alternatives for permanent wound closure restore the anatomy and physiology of uninjured skin.					
35917370	7	8	theme	engineered	1125:1134	arg1	skin					1136:1139	an autologous engineered skin substitute	1111:1150	an autologous engineered skin substitute (ESS)	1111:1156	More recently, an autologous engineered skin substitute (ESS) has been reported and includes a C-GAG polymer populated with fibroblasts and keratinocytes which form basement membrane.					
35917370	7	8	theme	engineered	1125:1134	arg1	ESS					1153:1155	ESS	1153:1155	ESS	1153:1155	More recently, an autologous engineered skin substitute (ESS) has been reported and includes a C-GAG polymer populated with fibroblasts and keratinocytes which form basement membrane.					
35917370	8	9	theme	wound	1369:1373	arg1	closure					1375:1381	stable wound closure	1362:1381	stable wound closure that is smooth, soft, and strong	1362:1414	ESS can be applied clinically over a vascularized dermal substitute and generates stable wound closure that is smooth, soft, and strong.					
35917370	10	10	theme	wound	1589:1593	arg1	healing					1595:1601	wound healing	1589:1601	wound healing	1589:1601	Current alternatives act by mechanisms of wound healing, not by developmental biology by which skin forms in utero with pigment, hair, sweat and sebaceous glands, microvasculature, and nerve.					
35917370	11	11	dep	structures	1802:1811	arg1	the					1791:1793	the	1791:1793	the	1791:1793	Until full-thickness burns are restored with all of the normal structures and functions of uninjured skin, regenerative medicine of skin will remain an ambitious aspiration for future researchers and engineers to achieve.					
35917370	8	12	theme	stable	1362:1367	arg1	closure					1375:1381	stable wound closure	1362:1381	stable wound closure that is smooth, soft, and strong	1362:1414	ESS can be applied clinically over a vascularized dermal substitute and generates stable wound closure that is smooth, soft, and strong.					
35917370	7	13	theme	basement	1261:1268	arg1	membrane					1270:1277	basement membrane	1261:1277	basement membrane	1261:1277	More recently, an autologous engineered skin substitute (ESS) has been reported and includes a C-GAG polymer populated with fibroblasts and keratinocytes which form basement membrane.					
35917370	2	14	dep	extensive	343:351	arg1	deep					354:357	deep	354:357	deep	354:357	Historically, split-thickness skin autograft (STAG) has served as the prevailing standard of care for closure of extensive, deep burns.					
35917370	10	15	theme	sweat	1682:1686	arg1	glands					1702:1707	sweat and sebaceous glands	1682:1707	glands	1702:1707	Current alternatives act by mechanisms of wound healing, not by developmental biology by which skin forms in utero with pigment, hair, sweat and sebaceous glands, microvasculature, and nerve.					
35917370	7	16	theme	C-GAG	1191:1195	arg1	polymer					1197:1203	a C-GAG polymer	1189:1203	a C-GAG polymer populated with fibroblasts and keratinocytes which form basement membrane	1189:1277	More recently, an autologous engineered skin substitute (ESS) has been reported and includes a C-GAG polymer populated with fibroblasts and keratinocytes which form basement membrane.					
35917370	9	17	theme	wound	1479:1483	arg1	closure					1485:1491	permanent wound closure	1469:1491	permanent wound closure	1469:1491	Despite these advances, no current alternatives for permanent wound closure restore the anatomy and physiology of uninjured skin.					
35917370	0	18	dep	Excised	100:106	arg1	Full-Thickness					109:122	Full-Thickness	109:122	Full-Thickness	109:122	Composition and Performance of Autologous Engineered Skin Substitutes for Repair or Regeneration of Excised, Full-Thickness Burns.					
35917370	1	19	theme	burn	172:175	arg1	injuries					177:184	burn injuries	172:184	burn injuries	172:184	Prompt and permanent wound closure after burn injuries remains a requirement for patient recovery.					
35917370	5	20	theme	Cultured	756:763	arg1	autografts					776:785	Cultured epithelial autografts	756:785	Cultured epithelial autografts (CEA)	756:791	Cultured epithelial autografts (CEA) promoted epidermal closure of wounds but were not stable during long-term recovery.					
35917370	5	20	theme	Cultured	756:763	arg1	CEA					788:790	CEA	788:790	CEA	788:790	Cultured epithelial autografts (CEA) promoted epidermal closure of wounds but were not stable during long-term recovery.					
35917370	7	21	theme	autologous	1114:1123	arg1	skin					1136:1139	an autologous engineered skin substitute	1111:1150	an autologous engineered skin substitute (ESS)	1111:1156	More recently, an autologous engineered skin substitute (ESS) has been reported and includes a C-GAG polymer populated with fibroblasts and keratinocytes which form basement membrane.					
35917370	7	21	theme	autologous	1114:1123	arg1	ESS					1153:1155	ESS	1153:1155	ESS	1153:1155	More recently, an autologous engineered skin substitute (ESS) has been reported and includes a C-GAG polymer populated with fibroblasts and keratinocytes which form basement membrane.					
35917370	7	22	dep	skin	1136:1139	arg1	substitute					1141:1150	substitute	1141:1150	substitute	1141:1150	More recently, an autologous engineered skin substitute (ESS) has been reported and includes a C-GAG polymer populated with fibroblasts and keratinocytes which form basement membrane.					
35917370	9	23	theme	current	1444:1450	arg1	alternatives					1452:1463	no current alternatives	1441:1463	no current alternatives for permanent wound closure	1441:1491	Despite these advances, no current alternatives for permanent wound closure restore the anatomy and physiology of uninjured skin.					
35917370	3	24	theme	STAG	374:377	arg1	availability					379:390	STAG availability	374:390	STAG availability	374:390	Because STAG availability may be insufficient in life-threatening burns, alternatives have been evaluated for safety and efficacy of wound closure.					
35917370	6	25	theme	uniform	976:982	arg1	repair					991:996	more uniform dermal repair	971:996	more uniform dermal repair	971:996	An acellular dermal substitute consisting of collagen and glycosaminoglycans (C-GAG) provided more uniform dermal repair, and reduced needs for epidermal harvesting but was subject to loss from microbial contamination.					
35917370	9	26	theme	skin	1541:1544	arg1	physiology					1517:1526	physiology	1517:1526	physiology	1517:1526	Despite these advances, no current alternatives for permanent wound closure restore the anatomy and physiology of uninjured skin.					
35917370	9	26	theme	skin	1541:1544	arg1	anatomy					1505:1511	anatomy	1505:1511	anatomy	1505:1511	Despite these advances, no current alternatives for permanent wound closure restore the anatomy and physiology of uninjured skin.					
35917370	11	27	theme	future	1916:1921	arg1	researchers					1923:1933	future researchers	1916:1933	future researchers	1916:1933	Until full-thickness burns are restored with all of the normal structures and functions of uninjured skin, regenerative medicine of skin will remain an ambitious aspiration for future researchers and engineers to achieve.					
35917370	3	28	theme	life-threatening	415:430	arg1	burns					432:436	life-threatening burns	415:436	life-threatening burns	415:436	Because STAG availability may be insufficient in life-threatening burns, alternatives have been evaluated for safety and efficacy of wound closure.					
35917370	5	29	theme	wounds	823:828	arg1	closure					812:818	epidermal closure	802:818	epidermal closure of wounds	802:828	Cultured epithelial autografts (CEA) promoted epidermal closure of wounds but were not stable during long-term recovery.					
35917370	4	30	theme	major	743:747	arg1	burns					749:753	major burns	743:753	major burns	743:753	Since the 1970s, alternatives consisting of cultured epidermal keratinocytes, and/or acellular dermal substitutes were studied and translated into services and devices that facilitated wound closure, survival, and recovery after major burns.					
35917370	6	31	theme	microbial	1071:1079	arg1	contamination					1081:1093	microbial contamination	1071:1093	microbial contamination	1071:1093	An acellular dermal substitute consisting of collagen and glycosaminoglycans (C-GAG) provided more uniform dermal repair, and reduced needs for epidermal harvesting but was subject to loss from microbial contamination.					
35917370	11	32	theme	normal	1795:1800	arg1	structures					1802:1811	normal structures	1795:1811	normal structures	1795:1811	Until full-thickness burns are restored with all of the normal structures and functions of uninjured skin, regenerative medicine of skin will remain an ambitious aspiration for future researchers and engineers to achieve.					
35917370	10	33	theme	developmental	1611:1623	arg1	biology					1625:1631	developmental biology	1611:1631	developmental biology by which skin forms in utero with pigment, hair, sweat and sebaceous glands, microvasculature, and nerve	1611:1736	Current alternatives act by mechanisms of wound healing, not by developmental biology by which skin forms in utero with pigment, hair, sweat and sebaceous glands, microvasculature, and nerve.					
35917370	11	34	theme	skin	1840:1843	arg1	functions					1817:1825	functions	1817:1825	functions	1817:1825	Until full-thickness burns are restored with all of the normal structures and functions of uninjured skin, regenerative medicine of skin will remain an ambitious aspiration for future researchers and engineers to achieve.					
35917370	11	34	theme	skin	1840:1843	arg1	structures					1802:1811	normal structures	1795:1811	normal structures	1795:1811	Until full-thickness burns are restored with all of the normal structures and functions of uninjured skin, regenerative medicine of skin will remain an ambitious aspiration for future researchers and engineers to achieve.					
35917370	9	35	theme	permanent	1469:1477	arg1	closure					1485:1491	permanent wound closure	1469:1491	permanent wound closure	1469:1491	Despite these advances, no current alternatives for permanent wound closure restore the anatomy and physiology of uninjured skin.					
35917370	11	36	theme	full-thickness	1745:1758	arg1	burns					1760:1764	full-thickness burns	1745:1764	full-thickness burns	1745:1764	Until full-thickness burns are restored with all of the normal structures and functions of uninjured skin, regenerative medicine of skin will remain an ambitious aspiration for future researchers and engineers to achieve.					
35917370	4	37	theme	epidermal	567:575	arg1	keratinocytes					577:589	cultured epidermal keratinocytes	558:589	cultured epidermal keratinocytes	558:589	Since the 1970s, alternatives consisting of cultured epidermal keratinocytes, and/or acellular dermal substitutes were studied and translated into services and devices that facilitated wound closure, survival, and recovery after major burns.					
35917370	0	38	theme	Burns	124:128	arg1	Regeneration					84:95	Regeneration	84:95	Regeneration	84:95	Composition and Performance of Autologous Engineered Skin Substitutes for Repair or Regeneration of Excised, Full-Thickness Burns.					
35917370	0	38	theme	Burns	124:128	arg1	Repair					74:79	Repair	74:79	Repair	74:79	Composition and Performance of Autologous Engineered Skin Substitutes for Repair or Regeneration of Excised, Full-Thickness Burns.					
35917370	3	39	theme	closure	505:511	arg1	efficacy					487:494	efficacy	487:494	efficacy	487:494	Because STAG availability may be insufficient in life-threatening burns, alternatives have been evaluated for safety and efficacy of wound closure.					
35917370	3	39	theme	closure	505:511	arg1	safety					476:481	safety	476:481	safety	476:481	Because STAG availability may be insufficient in life-threatening burns, alternatives have been evaluated for safety and efficacy of wound closure.					
35917370	1	40	theme	permanent	142:150	arg1	closure					158:164	permanent wound closure	142:164	permanent wound closure	142:164	Prompt and permanent wound closure after burn injuries remains a requirement for patient recovery.					
35917370	0	41	theme	Engineered	42:51	arg1	Skin					53:56	Autologous Engineered Skin	31:56	Autologous Engineered Skin	31:56	Composition and Performance of Autologous Engineered Skin Substitutes for Repair or Regeneration of Excised, Full-Thickness Burns.					
35917370	4	42	theme	cultured	558:565	arg1	keratinocytes					577:589	cultured epidermal keratinocytes	558:589	cultured epidermal keratinocytes	558:589	Since the 1970s, alternatives consisting of cultured epidermal keratinocytes, and/or acellular dermal substitutes were studied and translated into services and devices that facilitated wound closure, survival, and recovery after major burns.					
35917370	2	43	theme	split-thickness	244:258	arg1	autograft					265:273	split-thickness skin autograft	244:273	split-thickness skin autograft (STAG)	244:280	Historically, split-thickness skin autograft (STAG) has served as the prevailing standard of care for closure of extensive, deep burns.					
35917370	2	43	theme	split-thickness	244:258	arg1	standard					311:318	standard	311:318	standard	311:318	Historically, split-thickness skin autograft (STAG) has served as the prevailing standard of care for closure of extensive, deep burns.					
35917370	2	43	theme	split-thickness	244:258	arg1	STAG					276:279	STAG	276:279	STAG	276:279	Historically, split-thickness skin autograft (STAG) has served as the prevailing standard of care for closure of extensive, deep burns.					
35917370	1	44	theme	patient	212:218	arg1	recovery					220:227	patient recovery	212:227	patient recovery	212:227	Prompt and permanent wound closure after burn injuries remains a requirement for patient recovery.					
35917370	0	45	theme	Autologous	31:40	arg1	Skin					53:56	Autologous Engineered Skin	31:56	Autologous Engineered Skin	31:56	Composition and Performance of Autologous Engineered Skin Substitutes for Repair or Regeneration of Excised, Full-Thickness Burns.					
35917370	5	46	theme	long-term	857:865	arg1	recovery					867:874	long-term recovery	857:874	long-term recovery	857:874	Cultured epithelial autografts (CEA) promoted epidermal closure of wounds but were not stable during long-term recovery.					
35917370	5	47	theme	epidermal	802:810	arg1	closure					812:818	epidermal closure	802:818	epidermal closure of wounds	802:828	Cultured epithelial autografts (CEA) promoted epidermal closure of wounds but were not stable during long-term recovery.					
35917370	10	48	theme	healing	1595:1601	arg1	mechanisms					1575:1584	mechanisms	1575:1584	mechanisms of wound healing	1575:1601	Current alternatives act by mechanisms of wound healing, not by developmental biology by which skin forms in utero with pigment, hair, sweat and sebaceous glands, microvasculature, and nerve.					
35917370	11	49	theme	skin	1871:1874	arg1	medicine					1859:1866	regenerative medicine	1846:1866	regenerative medicine of skin	1846:1874	Until full-thickness burns are restored with all of the normal structures and functions of uninjured skin, regenerative medicine of skin will remain an ambitious aspiration for future researchers and engineers to achieve.					
35917370	0	50	theme	Skin	53:56	arg1	Performance					16:26	Performance	16:26	Performance	16:26	Composition and Performance of Autologous Engineered Skin Substitutes for Repair or Regeneration of Excised, Full-Thickness Burns.					
35917370	0	50	theme	Skin	53:56	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and Performance of Autologous Engineered Skin Substitutes for Repair or Regeneration of Excised, Full-Thickness Burns.					
35917370	8	51	dep	dermal	1330:1335	arg1	substitute					1337:1346	substitute	1337:1346	substitute	1337:1346	ESS can be applied clinically over a vascularized dermal substitute and generates stable wound closure that is smooth, soft, and strong.					
35917370	10	52	theme	sebaceous	1692:1700	arg1	glands					1702:1707	sweat and sebaceous glands	1682:1707	glands	1702:1707	Current alternatives act by mechanisms of wound healing, not by developmental biology by which skin forms in utero with pigment, hair, sweat and sebaceous glands, microvasculature, and nerve.					
35917370	11	53	theme	regenerative	1846:1857	arg1	medicine					1859:1866	regenerative medicine	1846:1866	regenerative medicine of skin	1846:1874	Until full-thickness burns are restored with all of the normal structures and functions of uninjured skin, regenerative medicine of skin will remain an ambitious aspiration for future researchers and engineers to achieve.					
35917370	4	54	theme	wound	699:703	arg1	closure					705:711	wound closure	699:711	wound closure	699:711	Since the 1970s, alternatives consisting of cultured epidermal keratinocytes, and/or acellular dermal substitutes were studied and translated into services and devices that facilitated wound closure, survival, and recovery after major burns.					
35917370	11	55	theme	uninjured	1830:1838	arg1	skin					1840:1843	uninjured skin	1830:1843	uninjured skin	1830:1843	Until full-thickness burns are restored with all of the normal structures and functions of uninjured skin, regenerative medicine of skin will remain an ambitious aspiration for future researchers and engineers to achieve.					
35917370	9	56	dep	anatomy	1505:1511	arg1	the					1501:1503	the	1501:1503	the	1501:1503	Despite these advances, no current alternatives for permanent wound closure restore the anatomy and physiology of uninjured skin.					
35917370	2	57	theme	care	323:326	arg1	autograft					265:273	split-thickness skin autograft	244:273	split-thickness skin autograft (STAG)	244:280	Historically, split-thickness skin autograft (STAG) has served as the prevailing standard of care for closure of extensive, deep burns.					
35917370	2	57	theme	care	323:326	arg1	standard					311:318	standard	311:318	standard	311:318	Historically, split-thickness skin autograft (STAG) has served as the prevailing standard of care for closure of extensive, deep burns.					
35917370	6	58	dep	dermal	890:895	arg1	substitute					897:906	substitute	897:906	substitute	897:906	An acellular dermal substitute consisting of collagen and glycosaminoglycans (C-GAG) provided more uniform dermal repair, and reduced needs for epidermal harvesting but was subject to loss from microbial contamination.					
35917370	5	59	theme	epithelial	765:774	arg1	autografts					776:785	Cultured epithelial autografts	756:785	Cultured epithelial autografts (CEA)	756:791	Cultured epithelial autografts (CEA) promoted epidermal closure of wounds but were not stable during long-term recovery.					
35917370	5	59	theme	epithelial	765:774	arg1	CEA					788:790	CEA	788:790	CEA	788:790	Cultured epithelial autografts (CEA) promoted epidermal closure of wounds but were not stable during long-term recovery.					
35917370	4	60	dep	dermal	609:614	arg1	substitutes					616:626	substitutes	616:626	substitutes	616:626	Since the 1970s, alternatives consisting of cultured epidermal keratinocytes, and/or acellular dermal substitutes were studied and translated into services and devices that facilitated wound closure, survival, and recovery after major burns.					
35917370	3	61	from	burns	432:436	arg1	insufficient					399:410	insufficient	399:410	insufficient	399:410	Because STAG availability may be insufficient in life-threatening burns, alternatives have been evaluated for safety and efficacy of wound closure.					
35917370	2	62	theme	burns	359:363	arg1	closure					332:338	closure	332:338	closure of extensive, deep burns	332:363	Historically, split-thickness skin autograft (STAG) has served as the prevailing standard of care for closure of extensive, deep burns.					
35917370	11	63	theme	ambitious	1891:1899	arg1	aspiration					1901:1910	an ambitious aspiration	1888:1910	an ambitious aspiration for future researchers and engineers to achieve	1888:1958	Until full-thickness burns are restored with all of the normal structures and functions of uninjured skin, regenerative medicine of skin will remain an ambitious aspiration for future researchers and engineers to achieve.					
35917370	6	64	dep	reduced	1003:1009	arg1	needs					1011:1015	needs	1011:1015	needs for epidermal harvesting	1011:1040	An acellular dermal substitute consisting of collagen and glycosaminoglycans (C-GAG) provided more uniform dermal repair, and reduced needs for epidermal harvesting but was subject to loss from microbial contamination.					
35917370	6	64	dep	reduced	1003:1009	arg1	subject					1050:1056	subject	1050:1056	subject	1050:1056	An acellular dermal substitute consisting of collagen and glycosaminoglycans (C-GAG) provided more uniform dermal repair, and reduced needs for epidermal harvesting but was subject to loss from microbial contamination.					
35917370	2	65	theme	skin	260:263	arg1	autograft					265:273	split-thickness skin autograft	244:273	split-thickness skin autograft (STAG)	244:280	Historically, split-thickness skin autograft (STAG) has served as the prevailing standard of care for closure of extensive, deep burns.					
35917370	2	65	theme	skin	260:263	arg1	standard					311:318	standard	311:318	standard	311:318	Historically, split-thickness skin autograft (STAG) has served as the prevailing standard of care for closure of extensive, deep burns.					
35917370	2	65	theme	skin	260:263	arg1	STAG					276:279	STAG	276:279	STAG	276:279	Historically, split-thickness skin autograft (STAG) has served as the prevailing standard of care for closure of extensive, deep burns.					
35917370	1	66	theme	wound	152:156	arg1	closure					158:164	permanent wound closure	142:164	permanent wound closure	142:164	Prompt and permanent wound closure after burn injuries remains a requirement for patient recovery.					
35917370	6	67	theme	epidermal	1021:1029	arg1	harvesting					1031:1040	epidermal harvesting	1021:1040	epidermal harvesting	1021:1040	An acellular dermal substitute consisting of collagen and glycosaminoglycans (C-GAG) provided more uniform dermal repair, and reduced needs for epidermal harvesting but was subject to loss from microbial contamination.					
36622346	2	0	theme	species	362:368	arg1	classification					370:383	the species classification	358:383	the species classification	358:383	These spirochetes exhibit large intraspecific variation, resulting in complex tabulations of serogroups/serovars that crisscross the species classification.					
36622346	7	1	theme	rfb	957:959	arg1	cluster					961:967	the rfb cluster	953:967	the rfb cluster	953:967	Whole-genome sequencing of pathogenic L. noguchii, including strains of different serogroups, reveals that the rfb cluster undergoes extensive horizontal gene transfer.					
36622346	0	2	theme	genetic	58:64	arg1	determinant					66:76	a genetic determinant	56:76	a genetic determinant of serovar identity	56:96	Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity.					
36622346	0	2	theme	genetic	58:64	arg1	transfer					11:18	Horizontal transfer	0:18	Horizontal transfer of the rfb cluster in Leptospira	0:51	Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity.					
36622346	0	3	from	transfer	11:18	arg1	Leptospira					42:51	Leptospira	42:51	Leptospira	42:51	Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity.					
36622346	5	4	theme	cumbersome	703:712	arg1	procedures					724:733	cumbersome serologic procedures	703:733	cumbersome serologic procedures	703:733	However, the genetic basis of intraspecies variability is not fully understood, constraining diagnostics/typing methods to cumbersome serologic procedures.					
36622346	9	5	theme	distinct	1257:1264	arg1	clusters					1270:1277	the distinct rfb clusters	1253:1277	the distinct rfb clusters	1253:1277	This work paves the way to genetic typing of Leptospira serovars, and to pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits.					
36622346	0	6	theme	serovar	81:87	arg1	identity					89:96	serovar identity	81:96	serovar identity	81:96	Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity.					
36622346	9	7	theme	host-specific	1289:1301	arg1	traits					1313:1318	host-specific virulence traits	1289:1318	host-specific virulence traits	1289:1318	This work paves the way to genetic typing of Leptospira serovars, and to pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits.					
36622346	5	8	theme	serologic	714:722	arg1	procedures					724:733	cumbersome serologic procedures	703:733	cumbersome serologic procedures	703:733	However, the genetic basis of intraspecies variability is not fully understood, constraining diagnostics/typing methods to cumbersome serologic procedures.					
36622346	8	9	theme	Leptospira	1045:1054	arg1	species					1056:1062	several Leptospira species	1037:1062	several Leptospira species	1037:1062	The rfb clusters from several Leptospira species disclose a univocal correspondence between gene composition and serovar identity.					
36622346	9	10	theme	specific	1231:1238	arg1	genes					1240:1244	pinpointing specific genes	1219:1244	pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits	1219:1318	This work paves the way to genetic typing of Leptospira serovars, and to pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits.					
36622346	4	11	theme	LPS	500:502	arg1	genes					526:530	Many LPS biosynthesis-encoding genes	495:530	Many LPS biosynthesis-encoding genes	495:530	Many LPS biosynthesis-encoding genes reside within the chromosomic rfb gene cluster.					
36622346	8	12	theme	serovar	1128:1134	arg1	identity					1136:1143	serovar identity	1128:1143	serovar identity	1128:1143	The rfb clusters from several Leptospira species disclose a univocal correspondence between gene composition and serovar identity.					
36622346	9	13	theme	Leptospira	1191:1200	arg1	serovars					1202:1209	Leptospira serovars	1191:1209	Leptospira serovars	1191:1209	This work paves the way to genetic typing of Leptospira serovars, and to pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits.					
36622346	7	14	theme	serogroups	928:937	arg1	strains					907:913	strains	907:913	strains of different serogroups	907:937	Whole-genome sequencing of pathogenic L. noguchii, including strains of different serogroups, reveals that the rfb cluster undergoes extensive horizontal gene transfer.					
36622346	9	15	theme	genetic	1173:1179	arg1	typing					1181:1186	genetic typing	1173:1186	genetic typing of Leptospira serovars	1173:1209	This work paves the way to genetic typing of Leptospira serovars, and to pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits.					
36622346	4	16	theme	Many	495:498	arg1	genes					526:530	Many LPS biosynthesis-encoding genes	495:530	Many LPS biosynthesis-encoding genes	495:530	Many LPS biosynthesis-encoding genes reside within the chromosomic rfb gene cluster.					
36622346	10	17	theme	molecular	1355:1363	arg1	mechanism					1365:1373	the molecular mechanism	1351:1373	the molecular mechanism of rfb transfer among Leptospira strains and species	1351:1426	Further research shall unveil the molecular mechanism of rfb transfer among Leptospira strains and species.					
36622346	2	18	theme	intraspecific	261:273	arg1	variation					275:283	large intraspecific variation	255:283	large intraspecific variation	255:283	These spirochetes exhibit large intraspecific variation, resulting in complex tabulations of serogroups/serovars that crisscross the species classification.					
36622346	6	19	theme	gene	757:760	arg1	content					762:768	the gene content	753:768	the gene content of the rfb cluster	753:787	We now show that the gene content of the rfb cluster strongly correlates with Leptospira serovar designation.					
36622346	0	20	theme	identity	89:96	arg1	determinant					66:76	a genetic determinant	56:76	a genetic determinant of serovar identity	56:96	Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity.					
36622346	0	20	theme	identity	89:96	arg1	transfer					11:18	Horizontal transfer	0:18	Horizontal transfer of the rfb cluster in Leptospira	0:51	Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity.					
36622346	8	21	from	species	1056:1062	arg1	clusters					1023:1030	The rfb clusters	1015:1030	The rfb clusters from several Leptospira species	1015:1062	The rfb clusters from several Leptospira species disclose a univocal correspondence between gene composition and serovar identity.					
36622346	2	22	theme	large	255:259	arg1	variation					275:283	large intraspecific variation	255:283	large intraspecific variation	255:283	These spirochetes exhibit large intraspecific variation, resulting in complex tabulations of serogroups/serovars that crisscross the species classification.					
36622346	10	23	theme	rfb	1378:1380	arg1	transfer					1382:1389	rfb transfer	1378:1389	rfb transfer	1378:1389	Further research shall unveil the molecular mechanism of rfb transfer among Leptospira strains and species.					
36622346	4	24	theme	biosynthesis-encoding	504:524	arg1	genes					526:530	Many LPS biosynthesis-encoding genes	495:530	Many LPS biosynthesis-encoding genes	495:530	Many LPS biosynthesis-encoding genes reside within the chromosomic rfb gene cluster.					
36622346	5	25	theme	diagnostics/typing	673:690	arg1	methods					692:698	diagnostics/typing methods	673:698	diagnostics/typing methods	673:698	However, the genetic basis of intraspecies variability is not fully understood, constraining diagnostics/typing methods to cumbersome serologic procedures.					
36622346	8	26	theme	univocal	1075:1082	arg1	correspondence					1084:1097	a univocal correspondence	1073:1097	a univocal correspondence between gene composition and serovar identity	1073:1143	The rfb clusters from several Leptospira species disclose a univocal correspondence between gene composition and serovar identity.					
36622346	7	27	theme	different	918:926	arg1	serogroups					928:937	different serogroups	918:937	different serogroups	918:937	Whole-genome sequencing of pathogenic L. noguchii, including strains of different serogroups, reveals that the rfb cluster undergoes extensive horizontal gene transfer.					
36622346	1	28	theme	serious	169:175	arg1	disease					177:183	serious disease	169:183	serious disease	169:183	Leptospira bacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans.					
36622346	0	29	theme	Horizontal	0:9	arg1	determinant					66:76	a genetic determinant	56:76	a genetic determinant of serovar identity	56:96	Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity.					
36622346	0	29	theme	Horizontal	0:9	arg1	transfer					11:18	Horizontal transfer	0:18	Horizontal transfer of the rfb cluster in Leptospira	0:51	Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity.					
36622346	5	30	theme	intraspecies	610:621	arg1	variability					623:633	intraspecies variability	610:633	intraspecies variability	610:633	However, the genetic basis of intraspecies variability is not fully understood, constraining diagnostics/typing methods to cumbersome serologic procedures.					
36622346	2	31	theme	complex	299:305	arg1	tabulations					307:317	complex tabulations	299:317	complex tabulations of serogroups/serovars that crisscross the species classification	299:383	These spirochetes exhibit large intraspecific variation, resulting in complex tabulations of serogroups/serovars that crisscross the species classification.					
36622346	8	32	theme	gene	1107:1110	arg1	composition					1112:1122	gene composition	1107:1122	gene composition	1107:1122	The rfb clusters from several Leptospira species disclose a univocal correspondence between gene composition and serovar identity.					
36622346	3	33	theme	Serovar	386:392	arg1	identity					394:401	Serovar identity	386:401	Serovar identity	386:401	Serovar identity, linked to biological/clinical phenotypes, depends on the structure of surface-exposed LPS.					
36622346	7	34	theme	gene	1000:1003	arg1	transfer					1005:1012	extensive horizontal gene transfer	979:1012	extensive horizontal gene transfer	979:1012	Whole-genome sequencing of pathogenic L. noguchii, including strains of different serogroups, reveals that the rfb cluster undergoes extensive horizontal gene transfer.					
36622346	5	35	theme	variability	623:633	arg1	basis					601:605	the genetic basis	589:605	the genetic basis of intraspecies variability	589:633	However, the genetic basis of intraspecies variability is not fully understood, constraining diagnostics/typing methods to cumbersome serologic procedures.					
36622346	9	36	theme	rfb	1266:1268	arg1	clusters					1270:1277	the distinct rfb clusters	1253:1277	the distinct rfb clusters	1253:1277	This work paves the way to genetic typing of Leptospira serovars, and to pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits.					
36622346	3	37	theme	LPS	490:492	arg1	structure					461:469	the structure	457:469	the structure of surface-exposed LPS	457:492	Serovar identity, linked to biological/clinical phenotypes, depends on the structure of surface-exposed LPS.					
36622346	8	38	theme	rfb	1019:1021	arg1	clusters					1023:1030	The rfb clusters	1015:1030	The rfb clusters from several Leptospira species	1015:1062	The rfb clusters from several Leptospira species disclose a univocal correspondence between gene composition and serovar identity.					
36622346	10	39	theme	Leptospira	1397:1406	arg1	strains					1408:1414	Leptospira strains	1397:1414	Leptospira strains	1397:1414	Further research shall unveil the molecular mechanism of rfb transfer among Leptospira strains and species.					
36622346	7	40	theme	noguchii	887:894	arg1	sequencing					859:868	Whole-genome sequencing	846:868	Whole-genome sequencing	846:868	Whole-genome sequencing of pathogenic L. noguchii, including strains of different serogroups, reveals that the rfb cluster undergoes extensive horizontal gene transfer.					
36622346	0	41	theme	cluster	31:37	arg1	determinant					66:76	a genetic determinant	56:76	a genetic determinant of serovar identity	56:96	Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity.					
36622346	0	41	theme	cluster	31:37	arg1	transfer					11:18	Horizontal transfer	0:18	Horizontal transfer of the rfb cluster in Leptospira	0:51	Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity.					
36622346	10	42	theme	transfer	1382:1389	arg1	mechanism					1365:1373	the molecular mechanism	1351:1373	the molecular mechanism of rfb transfer among Leptospira strains and species	1351:1426	Further research shall unveil the molecular mechanism of rfb transfer among Leptospira strains and species.					
36622346	9	43	theme	serovars	1202:1209	arg1	typing					1181:1186	genetic typing	1173:1186	genetic typing of Leptospira serovars	1173:1209	This work paves the way to genetic typing of Leptospira serovars, and to pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits.					
36622346	1	44	theme	broad	190:194	arg1	humans					221:226	humans	221:226	humans	221:226	Leptospira bacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans.					
36622346	1	44	theme	broad	190:194	arg1	range					196:200	a broad range	188:200	a broad range of hosts including humans	188:226	Leptospira bacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans.					
36622346	0	45	theme	rfb	27:29	arg1	cluster					31:37	the rfb cluster	23:37	the rfb cluster	23:37	Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity.					
36622346	4	46	theme	gene	566:569	arg1	cluster					571:577	the chromosomic rfb gene cluster	546:577	the chromosomic rfb gene cluster	546:577	Many LPS biosynthesis-encoding genes reside within the chromosomic rfb gene cluster.					
36622346	4	47	theme	chromosomic	550:560	arg1	cluster					571:577	the chromosomic rfb gene cluster	546:577	the chromosomic rfb gene cluster	546:577	Many LPS biosynthesis-encoding genes reside within the chromosomic rfb gene cluster.					
36622346	3	48	theme	surface-exposed	474:488	arg1	LPS					490:492	surface-exposed LPS	474:492	surface-exposed LPS	474:492	Serovar identity, linked to biological/clinical phenotypes, depends on the structure of surface-exposed LPS.					
36622346	4	49	theme	rfb	562:564	arg1	cluster					571:577	the chromosomic rfb gene cluster	546:577	the chromosomic rfb gene cluster	546:577	Many LPS biosynthesis-encoding genes reside within the chromosomic rfb gene cluster.					
36622346	1	50	theme	Leptospira	99:108	arg1	bacteria					110:117	Leptospira bacteria	99:117	Leptospira bacteria	99:117	Leptospira bacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans.					
36622346	3	51	theme	biological/clinical	414:432	arg1	phenotypes					434:443	biological/clinical phenotypes	414:443	biological/clinical phenotypes	414:443	Serovar identity, linked to biological/clinical phenotypes, depends on the structure of surface-exposed LPS.					
36622346	9	52	theme	virulence	1303:1311	arg1	traits					1313:1318	host-specific virulence traits	1289:1318	host-specific virulence traits	1289:1318	This work paves the way to genetic typing of Leptospira serovars, and to pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits.					
36622346	3	53	attach	linked	404:409	arg1	phenotypes					434:443	biological/clinical phenotypes	414:443	biological/clinical phenotypes	414:443	Serovar identity, linked to biological/clinical phenotypes, depends on the structure of surface-exposed LPS.					
36622346	3	53	attach	linked	404:409	arg2	identity					394:401	Serovar identity	386:401	Serovar identity	386:401	Serovar identity, linked to biological/clinical phenotypes, depends on the structure of surface-exposed LPS.					
36622346	7	54	theme	extensive	979:987	arg1	transfer					1005:1012	extensive horizontal gene transfer	979:1012	extensive horizontal gene transfer	979:1012	Whole-genome sequencing of pathogenic L. noguchii, including strains of different serogroups, reveals that the rfb cluster undergoes extensive horizontal gene transfer.					
36622346	6	55	theme	serovar	825:831	arg1	designation					833:843	Leptospira serovar designation	814:843	Leptospira serovar designation	814:843	We now show that the gene content of the rfb cluster strongly correlates with Leptospira serovar designation.					
36622346	5	56	theme	genetic	593:599	arg1	basis					601:605	the genetic basis	589:605	the genetic basis of intraspecies variability	589:633	However, the genetic basis of intraspecies variability is not fully understood, constraining diagnostics/typing methods to cumbersome serologic procedures.					
36622346	2	57	theme	serogroups/serovars	322:340	arg1	tabulations					307:317	complex tabulations	299:317	complex tabulations of serogroups/serovars that crisscross the species classification	299:383	These spirochetes exhibit large intraspecific variation, resulting in complex tabulations of serogroups/serovars that crisscross the species classification.					
36622346	7	58	theme	horizontal	989:998	arg1	transfer					1005:1012	extensive horizontal gene transfer	979:1012	extensive horizontal gene transfer	979:1012	Whole-genome sequencing of pathogenic L. noguchii, including strains of different serogroups, reveals that the rfb cluster undergoes extensive horizontal gene transfer.					
36622346	9	59	theme	pinpointing	1219:1229	arg1	genes					1240:1244	pinpointing specific genes	1219:1244	pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits	1219:1318	This work paves the way to genetic typing of Leptospira serovars, and to pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits.					
36622346	6	60	theme	Leptospira	814:823	arg1	designation					833:843	Leptospira serovar designation	814:843	Leptospira serovar designation	814:843	We now show that the gene content of the rfb cluster strongly correlates with Leptospira serovar designation.					
36622346	6	61	theme	cluster	781:787	arg1	content					762:768	the gene content	753:768	the gene content of the rfb cluster	753:787	We now show that the gene content of the rfb cluster strongly correlates with Leptospira serovar designation.					
36622346	1	62	theme	numerous	128:135	arg1	species					137:143	numerous species	128:143	numerous species	128:143	Leptospira bacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans.					
36622346	10	63	theme	Further	1321:1327	arg1	research					1329:1336	Further research	1321:1336	Further research	1321:1336	Further research shall unveil the molecular mechanism of rfb transfer among Leptospira strains and species.					
36622346	1	64	theme	hosts	205:209	arg1	humans					221:226	humans	221:226	humans	221:226	Leptospira bacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans.					
36622346	1	64	theme	hosts	205:209	arg1	range					196:200	a broad range	188:200	a broad range of hosts including humans	188:226	Leptospira bacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans.					
36622346	6	65	theme	rfb	777:779	arg1	cluster					781:787	the rfb cluster	773:787	the rfb cluster	773:787	We now show that the gene content of the rfb cluster strongly correlates with Leptospira serovar designation.					
36622346	1	66	theme	species	137:143	arg1	several					146:152	several	146:152	several	146:152	Leptospira bacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans.					
36622346	8	67	theme	several	1037:1043	arg1	species					1056:1062	several Leptospira species	1037:1062	several Leptospira species	1037:1062	The rfb clusters from several Leptospira species disclose a univocal correspondence between gene composition and serovar identity.					
36622346	7	68	theme	Whole-genome	846:857	arg1	sequencing					859:868	Whole-genome sequencing	846:868	Whole-genome sequencing	846:868	Whole-genome sequencing of pathogenic L. noguchii, including strains of different serogroups, reveals that the rfb cluster undergoes extensive horizontal gene transfer.					
36113720	11	0	theme	range	1819:1823	arg1	delivery					1800:1807	the delivery	1796:1807	the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform	1796:1928	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	2	1	theme	macropore	290:298	arg1	network					300:306	the macropore network	286:306	the macropore network	286:306	However, due to their large pore size allowing for unrestricted diffusion in the macropore network, macroporous hydrogels alone are not able to efficiently capture and release biomolecules in a controlled manner.					
36113720	11	2	theme	device	1977:1982	arg1	loading					1962:1968	in situ (re)loading	1950:1968	in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release	1950:2075	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	3	3	theme	sustained	542:550	arg1	manner					567:572	a sustained and controlled manner	540:572	a sustained and controlled manner	540:572	There is thus a need for biofunctionalized, affinity-based gels that can efficiently load and release biomolecules in a sustained and controlled manner.					
36113720	1	4	theme	biomedical	191:200	arg1	field					202:206	the biomedical field	187:206	the biomedical field	187:206	Macroporous hydrogels possess a vast potential for various applications in the biomedical field.					
36113720	4	5	theme	controlled	659:668	arg1	capture					670:676	controlled capture	659:676	controlled capture	659:676	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	11	6	theme	several	1725:1731	arg1	challenges					1741:1750	several ongoing challenges	1725:1750	several ongoing challenges	1725:1750	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	5	7	theme	Ecoil-tagged	872:883	arg1	Factors					927:933	Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors	872:933	Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors	872:933	By conjugating the Kcoil peptide to the dextran backbone, we achieved controlled loading and release of Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors.					
36113720	6	8	theme	gels	971:974	arg1	behavior					955:962	the behavior	951:962	the behavior of the gels	951:974	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	9	dep	affinity	1095:1102	arg1	iv					1087:1088	iv	1087:1088	iv	1087:1088	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	11	10	dep	platform	1783:1790	arg1	i					1768:1768	i	1768:1768	i	1768:1768	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	10	11	theme	interconnected	1678:1691	arg1	hydrogels					1701:1709	fully interconnected dextran hydrogels	1672:1709	fully interconnected dextran hydrogels	1672:1709	STATEMENT OF SIGNIFICANCE: This work presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels.					
36113720	10	11	theme	interconnected	1678:1691	arg1	macroporous					1659:1669	macroporous	1659:1669	macroporous	1659:1669	STATEMENT OF SIGNIFICANCE: This work presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels.					
36113720	4	12	theme	affinity	637:644	arg1	pair					646:649	a E/K coiled-coil affinity pair	619:649	a E/K coiled-coil affinity pair	619:649	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	2	13	theme	pore	237:240	arg1	size					242:245	their large pore size	225:245	their large pore size allowing for unrestricted diffusion in the macropore network	225:306	However, due to their large pore size allowing for unrestricted diffusion in the macropore network, macroporous hydrogels alone are not able to efficiently capture and release biomolecules in a controlled manner.					
36113720	11	14	theme	in	1950:1951	arg1	loading					1962:1968	in situ (re)loading	1950:1968	in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release	1950:2075	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	4	15	theme	factors	701:707	arg1	delivery					682:689	delivery	682:689	delivery	682:689	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	4	15	theme	factors	701:707	arg1	capture					670:676	controlled capture	659:676	controlled capture	659:676	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	11	16	theme	molecules	1846:1854	arg1	range					1819:1823	a wide range	1812:1823	a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors	1812:1912	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	5	17	theme	Factors	927:933	arg1	release					861:867	release	861:867	release of Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors	861:933	By conjugating the Kcoil peptide to the dextran backbone, we achieved controlled loading and release of Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors.					
36113720	5	17	theme	Factors	927:933	arg1	loading					849:855	controlled loading	838:855	controlled loading	838:855	By conjugating the Kcoil peptide to the dextran backbone, we achieved controlled loading and release of Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors.					
36113720	12	18	theme	vast	2131:2134	arg1	potential					2136:2144	a vast potential	2129:2144	a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine	2129:2230	Altogether, our macroporous dextran hydrogels have a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine.					
36113720	1	19	theme	Macroporous	112:122	arg1	hydrogels					124:132	Macroporous hydrogels	112:132	Macroporous hydrogels	112:132	Macroporous hydrogels possess a vast potential for various applications in the biomedical field.					
36113720	2	20	theme	unrestricted	260:271	arg1	diffusion					273:281	unrestricted diffusion	260:281	unrestricted diffusion in the macropore network	260:306	However, due to their large pore size allowing for unrestricted diffusion in the macropore network, macroporous hydrogels alone are not able to efficiently capture and release biomolecules in a controlled manner.					
36113720	9	21	theme	versatile	1402:1410	arg1	platform					1428:1435	a versatile, highly tunable platform	1400:1435	a versatile, highly tunable platform for the controlled delivery of growth factors in biomedical applications	1400:1508	Altogether, we propose a versatile, highly tunable platform for the controlled delivery of growth factors in biomedical applications.					
36113720	11	22	dep	loading	1888:1894	arg1	ii					1858:1859	ii	1858:1859	ii	1858:1859	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	11	23	theme	growth	2043:2048	arg1	capture					2057:2063	growth factor capture	2043:2063	growth factor capture	2043:2063	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	11	24	theme	stable	1828:1833	arg1	loading					1888:1894	a passive, affinity-based loading	1862:1894	loading	1888:1894	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	11	24	theme	stable	1828:1833	arg1	molecules					1846:1854	stable, bioactive molecules	1828:1854	stable, bioactive molecules	1828:1854	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	7	25	theme	20-fold	1154:1160	arg1	increase					1162:1169	a 20-fold increase	1152:1169	a 20-fold increase in passive growth factor capture by macroporous dextran gels	1152:1230	We demonstrate that Kcoil grafting can produce a 20-fold increase in passive growth factor capture by macroporous dextran gels.					
36113720	1	26	theme	various	163:169	arg1	applications					171:182	various applications	163:182	various applications in the biomedical field	163:206	Macroporous hydrogels possess a vast potential for various applications in the biomedical field.					
36113720	12	27	theme	dextran	2106:2112	arg1	hydrogels					2114:2122	our macroporous dextran hydrogels	2090:2122	our macroporous dextran hydrogels	2090:2122	Altogether, our macroporous dextran hydrogels have a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine.					
36113720	1	28	from	applications	171:182	arg1	field					202:206	the biomedical field	187:206	the biomedical field	187:206	Macroporous hydrogels possess a vast potential for various applications in the biomedical field.					
36113720	5	29	theme	Endothelial	908:918	arg1	Factors					927:933	Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors	872:933	Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors	872:933	By conjugating the Kcoil peptide to the dextran backbone, we achieved controlled loading and release of Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors.					
36113720	11	30	theme	passive	1864:1870	arg1	loading					1888:1894	a passive, affinity-based loading	1862:1894	loading	1888:1894	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	11	30	theme	passive	1864:1870	arg1	molecules					1846:1854	stable, bioactive molecules	1828:1854	stable, bioactive molecules	1828:1854	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	3	31	theme	controlled	556:565	arg1	manner					567:572	a sustained and controlled manner	540:572	a sustained and controlled manner	540:572	There is thus a need for biofunctionalized, affinity-based gels that can efficiently load and release biomolecules in a sustained and controlled manner.					
36113720	6	32	dep	parameters	1001:1010	arg1	size					1027:1030	(i) macropore size	1013:1030	(i) macropore size	1013:1030	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	32	dep	parameters	1001:1010	arg1	affinity					1095:1102	(iv) E/K affinity	1086:1102	(iv) E/K affinity	1086:1102	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	32	dep	parameters	1001:1010	arg1	density					1053:1059	(ii) Kcoil grafting density	1033:1059	(ii) Kcoil grafting density	1033:1059	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	32	dep	parameters	1001:1010	arg1	parameters					1001:1010	four control parameters	988:1010	four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity	988:1102	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	32	dep	parameters	1001:1010	arg1	valency					1074:1080	(iii) Ecoil valency	1062:1080	(iii) Ecoil valency	1062:1080	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	2	33	theme	controlled	403:412	arg1	manner					414:419	a controlled manner	401:419	a controlled manner	401:419	However, due to their large pore size allowing for unrestricted diffusion in the macropore network, macroporous hydrogels alone are not able to efficiently capture and release biomolecules in a controlled manner.					
36113720	12	34	from	applications	2150:2161	arg1	delivery					2177:2184	controlled delivery	2166:2184	controlled delivery	2166:2184	Altogether, our macroporous dextran hydrogels have a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine.					
36113720	12	34	from	applications	2150:2161	arg1	engineering					2194:2204	tissue engineering	2187:2204	tissue engineering	2187:2204	Altogether, our macroporous dextran hydrogels have a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine.					
36113720	12	34	from	applications	2150:2161	arg1	medicine					2223:2230	regenerative medicine	2210:2230	regenerative medicine	2210:2230	Altogether, our macroporous dextran hydrogels have a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine.					
36113720	4	35	dep	capture	670:676	arg1	the					655:657	the	655:657	the	655:657	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	0	36	theme	Macroporous	0:10	arg1	hydrogels					20:28	Macroporous dextran hydrogels	0:28	Macroporous dextran hydrogels for controlled growth factor capture and delivery using coiled-coil interactions.	0:110	Macroporous dextran hydrogels for controlled growth factor capture and delivery using coiled-coil interactions.					
36113720	9	37	theme	growth	1468:1473	arg1	factors					1475:1481	growth factors	1468:1481	growth factors	1468:1481	Altogether, we propose a versatile, highly tunable platform for the controlled delivery of growth factors in biomedical applications.					
36113720	12	38	theme	regenerative	2210:2221	arg1	medicine					2223:2230	regenerative medicine	2210:2230	regenerative medicine	2210:2230	Altogether, our macroporous dextran hydrogels have a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine.					
36113720	4	39	theme	dextran	749:755	arg1	hydrogels					757:765	highly interconnected, macroporous dextran hydrogels	714:765	highly interconnected, macroporous dextran hydrogels	714:765	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	10	40	theme	growth	1587:1592	arg1	capture					1601:1607	growth factor capture	1587:1607	growth factor capture	1587:1607	STATEMENT OF SIGNIFICANCE: This work presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels.					
36113720	2	41	from	diffusion	273:281	arg1	network					300:306	the macropore network	286:306	the macropore network	286:306	However, due to their large pore size allowing for unrestricted diffusion in the macropore network, macroporous hydrogels alone are not able to efficiently capture and release biomolecules in a controlled manner.					
36113720	7	42	theme	dextran	1219:1225	arg1	gels					1227:1230	macroporous dextran gels	1207:1230	macroporous dextran gels	1207:1230	We demonstrate that Kcoil grafting can produce a 20-fold increase in passive growth factor capture by macroporous dextran gels.					
36113720	6	43	dep	density	1053:1059	arg1	ii					1034:1035	ii	1034:1035	ii	1034:1035	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	3	44	theme	biofunctionalized	447:463	arg1	gels					481:484	biofunctionalized, affinity-based gels	447:484	biofunctionalized, affinity-based gels that can efficiently load and release biomolecules in a sustained and controlled manner	447:572	There is thus a need for biofunctionalized, affinity-based gels that can efficiently load and release biomolecules in a sustained and controlled manner.					
36113720	0	45	theme	controlled	34:43	arg1	capture					59:65	controlled growth factor capture	34:65	controlled growth factor capture	34:65	Macroporous dextran hydrogels for controlled growth factor capture and delivery using coiled-coil interactions.					
36113720	5	46	theme	Kcoil	787:791	arg1	peptide					793:799	the Kcoil peptide	783:799	the Kcoil peptide	783:799	By conjugating the Kcoil peptide to the dextran backbone, we achieved controlled loading and release of Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors.					
36113720	11	47	theme	different	1999:2007	arg1	parameters					2017:2026	four different control parameters	1994:2026	four different control parameters	1994:2026	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	8	48	theme	loaded	1311:1316	arg1	factors					1325:1331	the loaded growth factors	1307:1331	the loaded growth factors	1307:1331	Furthermore, we demonstrate that our gels can release as little as 20% of the loaded growth factors over one week, while retaining bioactivity.					
36113720	10	49	theme	sustained	1613:1621	arg1	delivery					1623:1630	sustained delivery	1613:1630	sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels	1613:1709	STATEMENT OF SIGNIFICANCE: This work presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels.					
36113720	0	50	theme	factor	52:57	arg1	capture					59:65	controlled growth factor capture	34:65	controlled growth factor capture	34:65	Macroporous dextran hydrogels for controlled growth factor capture and delivery using coiled-coil interactions.					
36113720	6	51	theme	Ecoil	1068:1072	arg1	parameters					1001:1010	four control parameters	988:1010	four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity	988:1102	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	51	theme	Ecoil	1068:1072	arg1	valency					1074:1080	(iii) Ecoil valency	1062:1080	(iii) Ecoil valency	1062:1080	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	8	52	theme	factors	1325:1331	arg1	factors					1325:1331	the loaded growth factors	1307:1331	the loaded growth factors	1307:1331	Furthermore, we demonstrate that our gels can release as little as 20% of the loaded growth factors over one week, while retaining bioactivity.					
36113720	8	52	theme	factors	1325:1331	arg1	%					1302:1302	as little as 20%	1287:1302	as little as 20% of the loaded growth factors	1287:1331	Furthermore, we demonstrate that our gels can release as little as 20% of the loaded growth factors over one week, while retaining bioactivity.					
36113720	11	53	theme	affinity-based	1873:1886	arg1	loading					1888:1894	a passive, affinity-based loading	1862:1894	loading	1888:1894	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	11	53	theme	affinity-based	1873:1886	arg1	molecules					1846:1854	stable, bioactive molecules	1828:1854	stable, bioactive molecules	1828:1854	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	6	54	dep	valency	1074:1080	arg1	iii					1063:1065	iii	1063:1065	iii	1063:1065	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	7	55	theme	growth	1182:1187	arg1	factor					1189:1194	passive growth factor	1174:1194	passive growth factor capture by macroporous dextran gels	1174:1230	We demonstrate that Kcoil grafting can produce a 20-fold increase in passive growth factor capture by macroporous dextran gels.					
36113720	11	56	theme	versatile	1773:1781	arg1	platform					1783:1790	a versatile platform	1771:1790	: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform	1765:1928	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	7	57	from	increase	1162:1169	arg1	capture					1196:1202	passive growth factor capture	1174:1202	passive growth factor capture by macroporous dextran gels	1174:1230	We demonstrate that Kcoil grafting can produce a 20-fold increase in passive growth factor capture by macroporous dextran gels.					
36113720	6	58	theme	grafting	1044:1051	arg1	density					1053:1059	(ii) Kcoil grafting density	1033:1059	(ii) Kcoil grafting density	1033:1059	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	58	theme	grafting	1044:1051	arg1	parameters					1001:1010	four control parameters	988:1010	four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity	988:1102	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	12	59	theme	tissue	2187:2192	arg1	engineering					2194:2204	tissue engineering	2187:2204	tissue engineering	2187:2204	Altogether, our macroporous dextran hydrogels have a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine.					
36113720	1	60	contain	possess	134:140	arg2	potential					149:157	a vast potential	142:157	a vast potential for various applications in the biomedical field	142:206	Macroporous hydrogels possess a vast potential for various applications in the biomedical field.					
36113720	1	60	contain	possess	134:140	arg1	hydrogels					124:132	Macroporous hydrogels	112:132	Macroporous hydrogels	112:132	Macroporous hydrogels possess a vast potential for various applications in the biomedical field.					
36113720	11	61	dep	in	1950:1951	arg1	re					1959:1960	re	1959:1960	re	1959:1960	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	11	61	dep	in	1950:1951	arg1	situ					1953:1956	situ	1953:1956	situ	1953:1956	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	6	62	theme	E/K	1091:1093	arg1	affinity					1095:1102	(iv) E/K affinity	1086:1102	(iv) E/K affinity	1086:1102	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	62	theme	E/K	1091:1093	arg1	parameters					1001:1010	four control parameters	988:1010	four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity	988:1102	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	4	63	theme	pair	646:649	arg1	use					612:614	the use	608:614	the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels	608:765	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	10	64	dep	STATEMENT	1511:1519	arg1	presents					1548:1555	presents	1548:1555	presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels	1548:1709	STATEMENT OF SIGNIFICANCE: This work presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels.					
36113720	12	65	contain	have	2124:2127	arg1	hydrogels					2114:2122	our macroporous dextran hydrogels	2090:2122	our macroporous dextran hydrogels	2090:2122	Altogether, our macroporous dextran hydrogels have a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine.					
36113720	12	65	contain	have	2124:2127	arg2	potential					2136:2144	a vast potential	2129:2144	a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine	2129:2230	Altogether, our macroporous dextran hydrogels have a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine.					
36113720	11	66	theme	ongoing	1733:1739	arg1	challenges					1741:1750	several ongoing challenges	1725:1750	several ongoing challenges	1725:1750	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	2	67	theme	macroporous	309:319	arg1	hydrogels					321:329	macroporous hydrogels	309:329	macroporous hydrogels alone	309:335	However, due to their large pore size allowing for unrestricted diffusion in the macropore network, macroporous hydrogels alone are not able to efficiently capture and release biomolecules in a controlled manner.					
36113720	12	68	theme	controlled	2166:2175	arg1	delivery					2177:2184	controlled delivery	2166:2184	controlled delivery	2166:2184	Altogether, our macroporous dextran hydrogels have a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine.					
36113720	7	69	theme	macroporous	1207:1217	arg1	gels					1227:1230	macroporous dextran gels	1207:1230	macroporous dextran gels	1207:1230	We demonstrate that Kcoil grafting can produce a 20-fold increase in passive growth factor capture by macroporous dextran gels.					
36113720	5	70	theme	controlled	838:847	arg1	loading					849:855	controlled loading	838:855	controlled loading	838:855	By conjugating the Kcoil peptide to the dextran backbone, we achieved controlled loading and release of Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors.					
36113720	4	71	theme	coiled-coil	625:635	arg1	pair					646:649	a E/K coiled-coil affinity pair	619:649	a E/K coiled-coil affinity pair	619:649	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	10	72	theme	SIGNIFICANCE	1524:1535	arg1	STATEMENT					1511:1519	STATEMENT	1511:1519	STATEMENT OF SIGNIFICANCE: This work presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels.	1511:1710	STATEMENT OF SIGNIFICANCE: This work presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels.					
36113720	2	73	theme	large	231:235	arg1	size					242:245	their large pore size	225:245	their large pore size allowing for unrestricted diffusion in the macropore network	225:306	However, due to their large pore size allowing for unrestricted diffusion in the macropore network, macroporous hydrogels alone are not able to efficiently capture and release biomolecules in a controlled manner.					
36113720	5	74	theme	Growth	920:925	arg1	Factors					927:933	Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors	872:933	Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors	872:933	By conjugating the Kcoil peptide to the dextran backbone, we achieved controlled loading and release of Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors.					
36113720	4	75	theme	growth	694:699	arg1	factors					701:707	growth factors	694:707	growth factors	694:707	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	1	76	theme	vast	144:147	arg1	potential					149:157	a vast potential	142:157	a vast potential for various applications in the biomedical field	142:206	Macroporous hydrogels possess a vast potential for various applications in the biomedical field.					
36113720	10	77	theme	dextran	1693:1699	arg1	hydrogels					1701:1709	fully interconnected dextran hydrogels	1672:1709	fully interconnected dextran hydrogels	1672:1709	STATEMENT OF SIGNIFICANCE: This work presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels.					
36113720	10	77	theme	dextran	1693:1699	arg1	macroporous					1659:1669	macroporous	1659:1669	macroporous	1659:1669	STATEMENT OF SIGNIFICANCE: This work presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels.					
36113720	11	78	from	delivery	1800:1807	arg1	platform					1921:1928	the platform	1917:1928	the platform	1917:1928	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	4	79	theme	E/K	621:623	arg1	pair					646:649	a E/K coiled-coil affinity pair	619:649	a E/K coiled-coil affinity pair	619:649	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	5	80	theme	Epidermal	885:893	arg1	Factors					927:933	Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors	872:933	Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors	872:933	By conjugating the Kcoil peptide to the dextran backbone, we achieved controlled loading and release of Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors.					
36113720	11	81	theme	factor	2050:2055	arg1	capture					2057:2063	growth factor capture	2043:2063	growth factor capture	2043:2063	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	0	82	theme	coiled-coil	86:96	arg1	interactions					98:109	coiled-coil interactions	86:109	coiled-coil interactions	86:109	Macroporous dextran hydrogels for controlled growth factor capture and delivery using coiled-coil interactions.					
36113720	5	83	theme	Vascular	899:906	arg1	Factors					927:933	Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors	872:933	Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors	872:933	By conjugating the Kcoil peptide to the dextran backbone, we achieved controlled loading and release of Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors.					
36113720	9	84	from	delivery	1456:1463	arg1	applications					1497:1508	biomedical applications	1486:1508	biomedical applications	1486:1508	Altogether, we propose a versatile, highly tunable platform for the controlled delivery of growth factors in biomedical applications.					
36113720	6	85	theme	control	993:999	arg1	size					1027:1030	(i) macropore size	1013:1030	(i) macropore size	1013:1030	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	85	theme	control	993:999	arg1	affinity					1095:1102	(iv) E/K affinity	1086:1102	(iv) E/K affinity	1086:1102	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	85	theme	control	993:999	arg1	density					1053:1059	(ii) Kcoil grafting density	1033:1059	(ii) Kcoil grafting density	1033:1059	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	85	theme	control	993:999	arg1	parameters					1001:1010	four control parameters	988:1010	four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity	988:1102	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	85	theme	control	993:999	arg1	valency					1074:1080	(iii) Ecoil valency	1062:1080	(iii) Ecoil valency	1062:1080	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	7	86	theme	Kcoil	1125:1129	arg1	grafting					1131:1138	Kcoil grafting	1125:1138	Kcoil grafting	1125:1138	We demonstrate that Kcoil grafting can produce a 20-fold increase in passive growth factor capture by macroporous dextran gels.					
36113720	11	87	theme	factors	1906:1912	arg1	loading					1888:1894	a passive, affinity-based loading	1862:1894	loading	1888:1894	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	11	87	theme	factors	1906:1912	arg1	molecules					1846:1854	stable, bioactive molecules	1828:1854	stable, bioactive molecules	1828:1854	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	9	88	dep	versatile	1402:1410	arg1	tunable					1420:1426	tunable	1420:1426	tunable	1420:1426	Altogether, we propose a versatile, highly tunable platform for the controlled delivery of growth factors in biomedical applications.					
36113720	4	89	from	hydrogels	757:765	arg1	delivery					682:689	delivery	682:689	delivery	682:689	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	4	89	from	hydrogels	757:765	arg1	capture					670:676	controlled capture	659:676	controlled capture	659:676	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	9	90	theme	controlled	1445:1454	arg1	delivery					1456:1463	the controlled delivery	1441:1463	the controlled delivery of growth factors in biomedical applications	1441:1508	Altogether, we propose a versatile, highly tunable platform for the controlled delivery of growth factors in biomedical applications.					
36113720	6	91	theme	Kcoil	1038:1042	arg1	density					1053:1059	(ii) Kcoil grafting density	1033:1059	(ii) Kcoil grafting density	1033:1059	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	91	theme	Kcoil	1038:1042	arg1	parameters					1001:1010	four control parameters	988:1010	four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity	988:1102	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	12	92	theme	macroporous	2094:2104	arg1	hydrogels					2114:2122	our macroporous dextran hydrogels	2090:2122	our macroporous dextran hydrogels	2090:2122	Altogether, our macroporous dextran hydrogels have a vast potential for applications in controlled delivery, tissue engineering and regenerative medicine.					
36113720	6	93	dep	size	1027:1030	arg1	i					1014:1014	i	1014:1014	i	1014:1014	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	11	94	theme	wide	1814:1817	arg1	range					1819:1823	a wide range	1812:1823	a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors	1812:1912	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	0	95	theme	dextran	12:18	arg1	hydrogels					20:28	Macroporous dextran hydrogels	0:28	Macroporous dextran hydrogels for controlled growth factor capture and delivery using coiled-coil interactions.	0:110	Macroporous dextran hydrogels for controlled growth factor capture and delivery using coiled-coil interactions.					
36113720	4	96	theme	interconnected	721:734	arg1	hydrogels					757:765	highly interconnected, macroporous dextran hydrogels	714:765	highly interconnected, macroporous dextran hydrogels	714:765	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	11	97	theme	growth	1899:1904	arg1	factors					1906:1912	growth factors	1899:1912	growth factors	1899:1912	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	5	98	theme	dextran	808:814	arg1	backbone					816:823	the dextran backbone	804:823	the dextran backbone	804:823	By conjugating the Kcoil peptide to the dextran backbone, we achieved controlled loading and release of Ecoil-tagged Epidermal and Vascular Endothelial Growth Factors.					
36113720	9	99	theme	factors	1475:1481	arg1	delivery					1456:1463	the controlled delivery	1441:1463	the controlled delivery of growth factors in biomedical applications	1441:1508	Altogether, we propose a versatile, highly tunable platform for the controlled delivery of growth factors in biomedical applications.					
36113720	11	100	dep	tune	2038:2041	arg1	iii					1989:1991	iii	1989:1991	iii	1989:1991	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	4	101	theme	macroporous	737:747	arg1	hydrogels					757:765	highly interconnected, macroporous dextran hydrogels	714:765	highly interconnected, macroporous dextran hydrogels	714:765	For this purpose, we report here the use of a E/K coiled-coil affinity pair for the controlled capture and delivery of growth factors from highly interconnected, macroporous dextran hydrogels.					
36113720	0	102	theme	growth	45:50	arg1	capture					59:65	controlled growth factor capture	34:65	controlled growth factor capture	34:65	Macroporous dextran hydrogels for controlled growth factor capture and delivery using coiled-coil interactions.					
36113720	9	103	theme	biomedical	1486:1495	arg1	applications					1497:1508	biomedical applications	1486:1508	biomedical applications	1486:1508	Altogether, we propose a versatile, highly tunable platform for the controlled delivery of growth factors in biomedical applications.					
36113720	6	104	theme	macropore	1017:1025	arg1	size					1027:1030	(i) macropore size	1013:1030	(i) macropore size	1013:1030	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	6	104	theme	macropore	1017:1025	arg1	parameters					1001:1010	four control parameters	988:1010	four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity	988:1102	To finely tune the behavior of the gels, we propose four control parameters: (i) macropore size, (ii) Kcoil grafting density, (iii) Ecoil valency and (iv) E/K affinity.					
36113720	10	105	theme	tunable	1566:1572	arg1	platform					1574:1581	a highly tunable platform	1557:1581	a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels	1557:1709	STATEMENT OF SIGNIFICANCE: This work presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels.					
36113720	11	106	theme	control	2009:2015	arg1	parameters					2017:2026	four different control parameters	1994:2026	four different control parameters	1994:2026	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	3	107	dep	biofunctionalized	447:463	arg1	affinity-based					466:479	affinity-based	466:479	affinity-based	466:479	There is thus a need for biofunctionalized, affinity-based gels that can efficiently load and release biomolecules in a sustained and controlled manner.					
36113720	7	108	theme	factor	1189:1194	arg1	capture					1196:1202	passive growth factor capture	1174:1202	passive growth factor capture by macroporous dextran gels	1174:1230	We demonstrate that Kcoil grafting can produce a 20-fold increase in passive growth factor capture by macroporous dextran gels.					
36113720	8	109	theme	growth	1318:1323	arg1	factors					1325:1331	the loaded growth factors	1307:1331	the loaded growth factors	1307:1331	Furthermore, we demonstrate that our gels can release as little as 20% of the loaded growth factors over one week, while retaining bioactivity.					
36113720	10	110	theme	factor	1594:1599	arg1	capture					1601:1607	growth factor capture	1587:1607	growth factor capture	1587:1607	STATEMENT OF SIGNIFICANCE: This work presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels.					
36113720	11	111	dep	stable	1828:1833	arg1	bioactive					1836:1844	bioactive	1836:1844	bioactive	1836:1844	It addresses several ongoing challenges by presenting: (i) a versatile platform for the delivery of a wide range of stable, bioactive molecules, (ii) a passive, affinity-based loading of growth factors in the platform, paving the way for in situ (re)loading of the device and (iii) four different control parameters to finely tune growth factor capture and release.					
36113720	7	112	theme	passive	1174:1180	arg1	factor					1189:1194	passive growth factor	1174:1194	passive growth factor capture by macroporous dextran gels	1174:1230	We demonstrate that Kcoil grafting can produce a 20-fold increase in passive growth factor capture by macroporous dextran gels.					
36113720	10	113	theme	affinity	1638:1645	arg1	peptides					1647:1654	affinity peptides	1638:1654	affinity peptides	1638:1654	STATEMENT OF SIGNIFICANCE: This work presents a highly tunable platform for growth factor capture and sustained delivery using affinity peptides in macroporous, fully interconnected dextran hydrogels.					
36901705	6	0	theme	floral	914:919	arg1	display					921:927	some floral display and flower traits	909:945	display	921:927	RS parameters were correlated with some floral display and flower traits.					
36901705	15	1	dep	structure	1849:1857	arg1	the					1838:1840	the	1838:1840	the	1838:1840	Our results indicate that the flower structure and nectar composition of E. helleborine reflect its generalistic character and meet the requirements of a wide range of pollinators.					
36901705	1	2	theme	reproductive	264:275	arg1	RS					286:287	RS	286:287	RS	286:287	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	1	2	theme	reproductive	264:275	arg1	success					277:283	the reproductive success	260:283	shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations	252:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	13	3	theme	amino	1583:1587	arg1	AAs					1596:1598	AAs	1596:1598	AAs	1596:1598	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	13	3	theme	amino	1583:1587	arg1	acids					1589:1593	7 non-proteogenic amino acids	1565:1593	7 non-proteogenic amino acids (AAs)	1565:1599	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	8	4	theme	Flower	1018:1023	arg1	traits					1025:1030	Flower traits	1018:1030	Flower traits	1018:1030	Flower traits had a weak influence on RS (10 of the 192 cases analyzed).					
36901705	16	5	theme	pollinator	2070:2079	arg1	assemblages					2081:2091	pollinator assemblages	2070:2091	pollinator assemblages in particular populations	2070:2117	Simultaneously, the differentiation of flower traits suggests a variation in pollinator assemblages in particular populations.					
36901705	15	6	theme	nectar	1863:1868	arg1	composition					1870:1880	nectar composition	1863:1880	nectar composition	1863:1880	Our results indicate that the flower structure and nectar composition of E. helleborine reflect its generalistic character and meet the requirements of a wide range of pollinators.					
36901705	10	7	from	anthropogenic	1243:1255	arg1	populations					1277:1287	the natural populations	1265:1287	the natural populations	1265:1287	The nectar of E. helleborine is relatively diluted with a lower sugar concentration in the anthropogenic than in the natural populations.					
36901705	10	8	theme	helleborine	1169:1179	arg1	nectar					1156:1161	The nectar	1152:1161	The nectar of E. helleborine	1152:1179	The nectar of E. helleborine is relatively diluted with a lower sugar concentration in the anthropogenic than in the natural populations.					
36901705	9	9	from	trait	1110:1114	arg1	RS					1127:1128	shaping RS	1119:1128	shaping RS	1119:1128	The more important trait in shaping RS was nectar chemistry.					
36901705	1	10	from	shaping	252:258	arg1	populations					366:376	natural and anthropogenic populations	340:376	natural and anthropogenic populations	340:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	1	11	theme	flower	209:214	arg1	structure					216:224	flower structure	209:224	flower structure	209:224	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	8	12	dep	RS	1056:1057	arg1	10					1060:1061	10	1060:1061	10	1060:1061	Flower traits had a weak influence on RS (10 of the 192 cases analyzed).					
36901705	8	12	dep	RS	1056:1057	arg1	cases					1074:1078	the 192 cases	1066:1078	the 192 cases analyzed	1066:1087	Flower traits had a weak influence on RS (10 of the 192 cases analyzed).					
36901705	11	13	theme	sucrose	1332:1338	arg1	domination					1318:1327	domination	1318:1327	domination of sucrose over hexoses	1318:1351	In the natural populations, domination of sucrose over hexoses was found, while in the anthropogenic populations, hexoses were more abundant and the participation of sugars was balanced.					
36901705	13	14	theme	helleborine	1526:1536	arg1	nectar					1538:1543	E. helleborine nectar	1523:1543	E. helleborine nectar	1523:1543	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	17	15	theme	crucial	2285:2291	arg1	mechanisms					2260:2269	mechanisms	2260:2269	mechanisms	2260:2269	Knowledge about the factors influencing RS in distinct habitats helps to understand the evolutionary potential of species and to understand mechanisms and processes crucial for shaping interactions between plants and pollinators.					
36901705	10	16	theme	lower	1210:1214	arg1	concentration					1222:1234	a lower sugar concentration	1208:1234	a lower sugar concentration in the anthropogenic than in the natural populations	1208:1287	The nectar of E. helleborine is relatively diluted with a lower sugar concentration in the anthropogenic than in the natural populations.					
36901705	8	17	contain	had	1032:1034	arg1	traits					1025:1030	Flower traits	1018:1030	Flower traits	1018:1030	Flower traits had a weak influence on RS (10 of the 192 cases analyzed).					
36901705	8	17	contain	had	1032:1034	arg2	influence					1043:1051	a weak influence	1036:1051	a weak influence	1036:1051	Flower traits had a weak influence on RS (10 of the 192 cases analyzed).					
36901705	1	18	dep	shaping	252:258	arg1	RS					286:287	RS	286:287	RS	286:287	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	1	18	dep	shaping	252:258	arg1	success					277:283	the reproductive success	260:283	shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations	252:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	11	19	theme	sugars	1456:1461	arg1	balanced					1467:1474	balanced	1467:1474	balanced	1467:1474	In the natural populations, domination of sucrose over hexoses was found, while in the anthropogenic populations, hexoses were more abundant and the participation of sugars was balanced.					
36901705	11	19	theme	sugars	1456:1461	arg1	participation					1439:1451	the participation	1435:1451	the participation of sugars	1435:1461	In the natural populations, domination of sucrose over hexoses was found, while in the anthropogenic populations, hexoses were more abundant and the participation of sugars was balanced.					
36901705	1	20	theme	Epipactis	315:323	arg1	helleborine					325:335	the generalist orchid Epipactis helleborine	293:335	the generalist orchid Epipactis helleborine in natural and anthropogenic populations	293:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	0	21	from	Success	18:24	arg1	Populations					55:65	Natural and Anthropogenic Populations	29:65	Natural and Anthropogenic Populations of Generalist Epipactis helleborine	29:101	Does Reproductive Success in Natural and Anthropogenic Populations of Generalist Epipactis helleborine Depend on Flower Morphology and Nectar Composition?					
36901705	13	22	theme	acid	1648:1651	arg1	domination					1625:1634	a clear domination	1617:1634	a clear domination of glutamic acid	1617:1651	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	0	23	theme	Epipactis	81:89	arg1	helleborine					91:101	Generalist Epipactis helleborine	70:101	Generalist Epipactis helleborine	70:101	Does Reproductive Success in Natural and Anthropogenic Populations of Generalist Epipactis helleborine Depend on Flower Morphology and Nectar Composition?					
36901705	16	24	from	assemblages	2081:2091	arg1	populations					2107:2117	particular populations	2096:2117	particular populations	2096:2117	Simultaneously, the differentiation of flower traits suggests a variation in pollinator assemblages in particular populations.					
36901705	8	25	theme	weak	1038:1041	arg1	influence					1043:1051	a weak influence	1036:1051	a weak influence	1036:1051	Flower traits had a weak influence on RS (10 of the 192 cases analyzed).					
36901705	2	26	theme	E.	551:552	arg1	populations					566:576	E. helleborine populations	551:576	E. helleborine populations	551:576	We supposed that the distinct character of two groups of habitats creates different conditions for plant-pollinator relationships, thus influencing reproductive success in E. helleborine populations.					
36901705	17	27	theme	evolutionary	2208:2219	arg1	potential					2221:2229	the evolutionary potential	2204:2229	the evolutionary potential of species	2204:2240	Knowledge about the factors influencing RS in distinct habitats helps to understand the evolutionary potential of species and to understand mechanisms and processes crucial for shaping interactions between plants and pollinators.					
36901705	15	28	theme	E.	1885:1886	arg1	helleborine					1888:1898	E. helleborine	1885:1898	E. helleborine	1885:1898	Our results indicate that the flower structure and nectar composition of E. helleborine reflect its generalistic character and meet the requirements of a wide range of pollinators.					
36901705	14	29	from	relationships	1663:1675	arg1	populations					1742:1752	different populations	1732:1752	different populations	1732:1752	We noted relationships between some AAs and RS, but distinct AAs shaped RS in different populations, and their impact was independent of their participation.					
36901705	13	30	theme	clear	1619:1623	arg1	domination					1625:1634	a clear domination	1617:1634	a clear domination of glutamic acid	1617:1651	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	0	31	theme	Flower	113:118	arg1	Morphology					120:129	Flower Morphology	113:129	Flower Morphology	113:129	Does Reproductive Success in Natural and Anthropogenic Populations of Generalist Epipactis helleborine Depend on Flower Morphology and Nectar Composition?					
36901705	7	32	theme	Floral	948:953	arg1	display					955:961	Floral display	948:961	Floral display	948:961	Floral display influenced RS only in three anthropogenic populations.					
36901705	0	33	theme	Generalist	70:79	arg1	helleborine					91:101	Generalist Epipactis helleborine	70:101	Generalist Epipactis helleborine	70:101	Does Reproductive Success in Natural and Anthropogenic Populations of Generalist Epipactis helleborine Depend on Flower Morphology and Nectar Composition?					
36901705	17	34	theme	shaping	2297:2303	arg1	interactions					2305:2316	shaping interactions	2297:2316	shaping interactions between plants and pollinators	2297:2347	Knowledge about the factors influencing RS in distinct habitats helps to understand the evolutionary potential of species and to understand mechanisms and processes crucial for shaping interactions between plants and pollinators.					
36901705	15	35	theme	pollinators	1980:1990	arg1	range					1971:1975	a wide range	1964:1975	a wide range of pollinators	1964:1990	Our results indicate that the flower structure and nectar composition of E. helleborine reflect its generalistic character and meet the requirements of a wide range of pollinators.					
36901705	4	36	theme	natural	749:755	arg1	populations					757:767	the natural populations	745:767	the natural populations	745:767	On average, FRS was almost two times higher in the anthropogenic than in the natural populations.					
36901705	14	37	theme	different	1732:1740	arg1	populations					1742:1752	different populations	1732:1752	different populations	1732:1752	We noted relationships between some AAs and RS, but distinct AAs shaped RS in different populations, and their impact was independent of their participation.					
36901705	15	38	theme	range	1971:1975	arg1	requirements					1948:1959	the requirements	1944:1959	the requirements of a wide range of pollinators	1944:1990	Our results indicate that the flower structure and nectar composition of E. helleborine reflect its generalistic character and meet the requirements of a wide range of pollinators.					
36901705	16	39	theme	flower	2032:2037	arg1	traits					2039:2044	flower traits	2032:2044	flower traits	2032:2044	Simultaneously, the differentiation of flower traits suggests a variation in pollinator assemblages in particular populations.					
36901705	2	40	theme	plant-pollinator	478:493	arg1	relationships					495:507	plant-pollinator relationships	478:507	plant-pollinator relationships	478:507	We supposed that the distinct character of two groups of habitats creates different conditions for plant-pollinator relationships, thus influencing reproductive success in E. helleborine populations.					
36901705	9	41	theme	important	1100:1108	arg1	trait					1110:1114	The more important trait	1091:1114	The more important trait in shaping RS	1091:1128	The more important trait in shaping RS was nectar chemistry.					
36901705	9	41	theme	important	1100:1108	arg1	chemistry					1141:1149	nectar chemistry	1134:1149	nectar chemistry	1134:1149	The more important trait in shaping RS was nectar chemistry.					
36901705	0	42	theme	Natural	29:35	arg1	Populations					55:65	Natural and Anthropogenic Populations	29:65	Natural and Anthropogenic Populations of Generalist Epipactis helleborine	29:101	Does Reproductive Success in Natural and Anthropogenic Populations of Generalist Epipactis helleborine Depend on Flower Morphology and Nectar Composition?					
36901705	1	43	from	helleborine	325:335	arg1	populations					366:376	natural and anthropogenic populations	340:376	natural and anthropogenic populations	340:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	14	44	theme	AAs	1715:1717	arg1	RS					1726:1727	distinct AAs shaped RS	1706:1727	distinct AAs shaped RS	1706:1727	We noted relationships between some AAs and RS, but distinct AAs shaped RS in different populations, and their impact was independent of their participation.					
36901705	15	45	theme	generalistic	1912:1923	arg1	character					1925:1933	its generalistic character	1908:1933	its generalistic character	1908:1933	Our results indicate that the flower structure and nectar composition of E. helleborine reflect its generalistic character and meet the requirements of a wide range of pollinators.					
36901705	10	46	theme	E.	1166:1167	arg1	helleborine					1169:1179	E. helleborine	1166:1179	E. helleborine	1166:1179	The nectar of E. helleborine is relatively diluted with a lower sugar concentration in the anthropogenic than in the natural populations.					
36901705	9	47	theme	nectar	1134:1139	arg1	trait					1110:1114	The more important trait	1091:1114	The more important trait in shaping RS	1091:1128	The more important trait in shaping RS was nectar chemistry.					
36901705	9	47	theme	nectar	1134:1139	arg1	chemistry					1141:1149	nectar chemistry	1134:1149	nectar chemistry	1134:1149	The more important trait in shaping RS was nectar chemistry.					
36901705	1	48	theme	nectar	230:235	arg1	composition					237:247	nectar composition	230:247	nectar composition	230:247	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	6	49	theme	flower	933:938	arg1	traits					940:945	some floral display and flower traits	909:945	traits	940:945	RS parameters were correlated with some floral display and flower traits.					
36901705	16	50	from	variation	2057:2065	arg1	assemblages					2081:2091	pollinator assemblages	2070:2091	pollinator assemblages in particular populations	2070:2117	Simultaneously, the differentiation of flower traits suggests a variation in pollinator assemblages in particular populations.					
36901705	11	51	theme	anthropogenic	1377:1389	arg1	populations					1391:1401	the anthropogenic populations	1373:1401	the anthropogenic populations	1373:1401	In the natural populations, domination of sucrose over hexoses was found, while in the anthropogenic populations, hexoses were more abundant and the participation of sugars was balanced.					
36901705	13	52	theme	non-proteogenic	1567:1581	arg1	AAs					1596:1598	AAs	1596:1598	AAs	1596:1598	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	13	52	theme	non-proteogenic	1567:1581	arg1	acids					1589:1593	7 non-proteogenic amino acids	1565:1593	7 non-proteogenic amino acids (AAs)	1565:1599	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	1	53	theme	study	174:178	arg1	purpose					159:165	The purpose	155:165	The purpose of our study	155:178	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	5	54	theme	population	801:810	arg1	groups					812:817	the two population groups	793:817	the two population groups	793:817	The difference between the two population groups in PR was smaller but still statistically significant.					
36901705	1	55	theme	generalist	297:306	arg1	helleborine					325:335	the generalist orchid Epipactis helleborine	293:335	the generalist orchid Epipactis helleborine in natural and anthropogenic populations	293:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	15	56	theme	flower	1842:1847	arg1	structure					1849:1857	flower structure	1842:1857	flower structure	1842:1857	Our results indicate that the flower structure and nectar composition of E. helleborine reflect its generalistic character and meet the requirements of a wide range of pollinators.					
36901705	10	57	from	concentration	1222:1234	arg1	anthropogenic					1243:1255	anthropogenic	1243:1255	anthropogenic	1243:1255	The nectar of E. helleborine is relatively diluted with a lower sugar concentration in the anthropogenic than in the natural populations.					
36901705	0	58	theme	Anthropogenic	41:53	arg1	Populations					55:65	Natural and Anthropogenic Populations	29:65	Natural and Anthropogenic Populations of Generalist Epipactis helleborine	29:101	Does Reproductive Success in Natural and Anthropogenic Populations of Generalist Epipactis helleborine Depend on Flower Morphology and Nectar Composition?					
36901705	11	59	theme	natural	1297:1303	arg1	populations					1305:1315	the natural populations	1293:1315	the natural populations	1293:1315	In the natural populations, domination of sucrose over hexoses was found, while in the anthropogenic populations, hexoses were more abundant and the participation of sugars was balanced.					
36901705	0	60	theme	Nectar	135:140	arg1	Composition					142:152	Nectar Composition	135:152	Nectar Composition	135:152	Does Reproductive Success in Natural and Anthropogenic Populations of Generalist Epipactis helleborine Depend on Flower Morphology and Nectar Composition?					
36901705	3	61	theme	pollinaria	584:593	arg1	removal					595:601	pollinaria removal	584:601	pollinaria removal (PR)	584:606	Both pollinaria removal (PR) and fruiting (FRS) were differentiated between the populations.					
36901705	3	61	theme	pollinaria	584:593	arg1	PR					604:605	PR	604:605	PR	604:605	Both pollinaria removal (PR) and fruiting (FRS) were differentiated between the populations.					
36901705	2	62	theme	helleborine	554:564	arg1	populations					566:576	E. helleborine populations	551:576	E. helleborine populations	551:576	We supposed that the distinct character of two groups of habitats creates different conditions for plant-pollinator relationships, thus influencing reproductive success in E. helleborine populations.					
36901705	16	63	theme	particular	2096:2105	arg1	populations					2107:2117	particular populations	2096:2117	particular populations	2096:2117	Simultaneously, the differentiation of flower traits suggests a variation in pollinator assemblages in particular populations.					
36901705	1	64	theme	orchid	308:313	arg1	helleborine					325:335	the generalist orchid Epipactis helleborine	293:335	the generalist orchid Epipactis helleborine in natural and anthropogenic populations	293:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	11	65	from	populations	1391:1401	arg1	abundant					1422:1429	abundant	1422:1429	abundant	1422:1429	In the natural populations, domination of sucrose over hexoses was found, while in the anthropogenic populations, hexoses were more abundant and the participation of sugars was balanced.					
36901705	13	66	theme	E.	1523:1524	arg1	nectar					1538:1543	E. helleborine nectar	1523:1543	E. helleborine nectar	1523:1543	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	14	67	theme	participation	1797:1809	arg1	independent					1776:1786	independent	1776:1786	independent	1776:1786	We noted relationships between some AAs and RS, but distinct AAs shaped RS in different populations, and their impact was independent of their participation.					
36901705	2	68	from	success	540:546	arg1	populations					566:576	E. helleborine populations	551:576	E. helleborine populations	551:576	We supposed that the distinct character of two groups of habitats creates different conditions for plant-pollinator relationships, thus influencing reproductive success in E. helleborine populations.					
36901705	16	69	theme	traits	2039:2044	arg1	differentiation					2013:2027	the differentiation	2009:2027	the differentiation of flower traits	2009:2044	Simultaneously, the differentiation of flower traits suggests a variation in pollinator assemblages in particular populations.					
36901705	10	70	theme	sugar	1216:1220	arg1	concentration					1222:1234	a lower sugar concentration	1208:1234	a lower sugar concentration in the anthropogenic than in the natural populations	1208:1287	The nectar of E. helleborine is relatively diluted with a lower sugar concentration in the anthropogenic than in the natural populations.					
36901705	5	71	from	difference	774:783	arg1	PR					822:823	PR	822:823	PR	822:823	The difference between the two population groups in PR was smaller but still statistically significant.					
36901705	1	72	from	role	201:204	arg1	shaping					252:258	shaping the reproductive success (RS)	252:288	shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations	252:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	10	73	from	populations	1277:1287	arg1	anthropogenic					1243:1255	anthropogenic	1243:1255	anthropogenic	1243:1255	The nectar of E. helleborine is relatively diluted with a lower sugar concentration in the anthropogenic than in the natural populations.					
36901705	14	74	from	RS	1698:1699	arg1	populations					1742:1752	different populations	1732:1752	different populations	1732:1752	We noted relationships between some AAs and RS, but distinct AAs shaped RS in different populations, and their impact was independent of their participation.					
36901705	11	75	located	found	1357:1361	arg2	domination					1318:1327	domination	1318:1327	domination of sucrose over hexoses	1318:1351	In the natural populations, domination of sucrose over hexoses was found, while in the anthropogenic populations, hexoses were more abundant and the participation of sugars was balanced.					
36901705	11	75	located	found	1357:1361	arg1	populations					1305:1315	the natural populations	1293:1315	the natural populations	1293:1315	In the natural populations, domination of sucrose over hexoses was found, while in the anthropogenic populations, hexoses were more abundant and the participation of sugars was balanced.					
36901705	1	76	theme	structure	216:224	arg1	role					201:204	the role	197:204	the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations	197:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	0	77	theme	helleborine	91:101	arg1	Populations					55:65	Natural and Anthropogenic Populations	29:65	Natural and Anthropogenic Populations of Generalist Epipactis helleborine	29:101	Does Reproductive Success in Natural and Anthropogenic Populations of Generalist Epipactis helleborine Depend on Flower Morphology and Nectar Composition?					
36901705	1	78	theme	helleborine	325:335	arg1	shaping					252:258	shaping the reproductive success (RS)	252:288	shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations	252:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	7	79	theme	anthropogenic	991:1003	arg1	populations					1005:1015	three anthropogenic populations	985:1015	three anthropogenic populations	985:1015	Floral display influenced RS only in three anthropogenic populations.					
36901705	4	80	theme	two	699:701	arg1	times					703:707	times	703:707	times	703:707	On average, FRS was almost two times higher in the anthropogenic than in the natural populations.					
36901705	1	81	theme	natural	340:346	arg1	populations					366:376	natural and anthropogenic populations	340:376	natural and anthropogenic populations	340:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	4	82	dep	times	703:707	arg1	higher					709:714	higher	709:714	higher	709:714	On average, FRS was almost two times higher in the anthropogenic than in the natural populations.					
36901705	15	83	theme	helleborine	1888:1898	arg1	composition					1870:1880	nectar composition	1863:1880	nectar composition	1863:1880	Our results indicate that the flower structure and nectar composition of E. helleborine reflect its generalistic character and meet the requirements of a wide range of pollinators.					
36901705	15	83	theme	helleborine	1888:1898	arg1	structure					1849:1857	flower structure	1842:1857	flower structure	1842:1857	Our results indicate that the flower structure and nectar composition of E. helleborine reflect its generalistic character and meet the requirements of a wide range of pollinators.					
36901705	10	84	theme	natural	1269:1275	arg1	populations					1277:1287	the natural populations	1265:1287	the natural populations	1265:1287	The nectar of E. helleborine is relatively diluted with a lower sugar concentration in the anthropogenic than in the natural populations.					
36901705	1	85	theme	anthropogenic	352:364	arg1	populations					366:376	natural and anthropogenic populations	340:376	natural and anthropogenic populations	340:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	2	86	theme	reproductive	527:538	arg1	success					540:546	reproductive success	527:546	reproductive success in E. helleborine populations	527:576	We supposed that the distinct character of two groups of habitats creates different conditions for plant-pollinator relationships, thus influencing reproductive success in E. helleborine populations.					
36901705	17	87	theme	species	2234:2240	arg1	potential					2221:2229	the evolutionary potential	2204:2229	the evolutionary potential of species	2204:2240	Knowledge about the factors influencing RS in distinct habitats helps to understand the evolutionary potential of species and to understand mechanisms and processes crucial for shaping interactions between plants and pollinators.					
36901705	0	88	theme	Reproductive	5:16	arg1	Success					18:24	Reproductive Success	5:24	Reproductive Success in Natural and Anthropogenic Populations of Generalist Epipactis helleborine	5:101	Does Reproductive Success in Natural and Anthropogenic Populations of Generalist Epipactis helleborine Depend on Flower Morphology and Nectar Composition?					
36901705	2	89	theme	habitats	436:443	arg1	habitats					436:443	habitats	436:443	habitats	436:443	We supposed that the distinct character of two groups of habitats creates different conditions for plant-pollinator relationships, thus influencing reproductive success in E. helleborine populations.					
36901705	2	89	theme	habitats	436:443	arg1	groups					426:431	two groups	422:431	two groups of habitats	422:443	We supposed that the distinct character of two groups of habitats creates different conditions for plant-pollinator relationships, thus influencing reproductive success in E. helleborine populations.					
36901705	13	90	theme	glutamic	1639:1646	arg1	acid					1648:1651	glutamic acid	1639:1651	glutamic acid	1639:1651	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	17	91	theme	distinct	2166:2173	arg1	habitats					2175:2182	distinct habitats	2166:2182	distinct habitats	2166:2182	Knowledge about the factors influencing RS in distinct habitats helps to understand the evolutionary potential of species and to understand mechanisms and processes crucial for shaping interactions between plants and pollinators.					
36901705	8	92	theme	cases	1074:1078	arg1	10					1060:1061	10	1060:1061	10	1060:1061	Flower traits had a weak influence on RS (10 of the 192 cases analyzed).					
36901705	8	92	theme	cases	1074:1078	arg1	cases					1074:1078	the 192 cases	1066:1078	the 192 cases analyzed	1066:1087	Flower traits had a weak influence on RS (10 of the 192 cases analyzed).					
36901705	15	93	theme	wide	1966:1969	arg1	range					1971:1975	a wide range	1964:1975	a wide range of pollinators	1964:1990	Our results indicate that the flower structure and nectar composition of E. helleborine reflect its generalistic character and meet the requirements of a wide range of pollinators.					
36901705	2	94	theme	groups	426:431	arg1	character					409:417	the distinct character	396:417	the distinct character of two groups of habitats	396:443	We supposed that the distinct character of two groups of habitats creates different conditions for plant-pollinator relationships, thus influencing reproductive success in E. helleborine populations.					
36901705	6	95	theme	RS	874:875	arg1	parameters					877:886	RS parameters	874:886	RS parameters	874:886	RS parameters were correlated with some floral display and flower traits.					
36901705	11	96	from	abundant	1422:1429	arg1	populations					1391:1401	the anthropogenic populations	1373:1401	the anthropogenic populations	1373:1401	In the natural populations, domination of sucrose over hexoses was found, while in the anthropogenic populations, hexoses were more abundant and the participation of sugars was balanced.					
36901705	2	97	theme	distinct	400:407	arg1	character					409:417	the distinct character	396:417	the distinct character of two groups of habitats	396:443	We supposed that the distinct character of two groups of habitats creates different conditions for plant-pollinator relationships, thus influencing reproductive success in E. helleborine populations.					
36901705	14	98	from	RS	1726:1727	arg1	populations					1742:1752	different populations	1732:1752	different populations	1732:1752	We noted relationships between some AAs and RS, but distinct AAs shaped RS in different populations, and their impact was independent of their participation.					
36901705	14	99	theme	shaped	1719:1724	arg1	RS					1726:1727	distinct AAs shaped RS	1706:1727	distinct AAs shaped RS	1706:1727	We noted relationships between some AAs and RS, but distinct AAs shaped RS in different populations, and their impact was independent of their participation.					
36901705	13	100	located	found	1606:1610	arg2	acids					1589:1593	7 non-proteogenic amino acids	1565:1593	7 non-proteogenic amino acids (AAs)	1565:1599	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	13	100	located	found	1606:1610	arg1	nectar					1538:1543	E. helleborine nectar	1523:1543	E. helleborine nectar	1523:1543	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	13	100	located	found	1606:1610	arg2	proteogenic					1549:1559	20 proteogenic	1546:1559	20 proteogenic	1546:1559	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	13	100	located	found	1606:1610	arg2	AAs					1596:1598	AAs	1596:1598	AAs	1596:1598	In E. helleborine nectar, 20 proteogenic and 7 non-proteogenic amino acids (AAs) were found with a clear domination of glutamic acid.					
36901705	2	101	theme	different	453:461	arg1	conditions					463:472	different conditions	453:472	different conditions for plant-pollinator relationships	453:507	We supposed that the distinct character of two groups of habitats creates different conditions for plant-pollinator relationships, thus influencing reproductive success in E. helleborine populations.					
36901705	9	102	theme	shaping	1119:1125	arg1	RS					1127:1128	shaping RS	1119:1128	shaping RS	1119:1128	The more important trait in shaping RS was nectar chemistry.					
36901705	14	103	theme	distinct	1706:1713	arg1	RS					1726:1727	distinct AAs shaped RS	1706:1727	distinct AAs shaped RS	1706:1727	We noted relationships between some AAs and RS, but distinct AAs shaped RS in different populations, and their impact was independent of their participation.					
36901705	1	104	from	populations	366:376	arg1	shaping					252:258	shaping the reproductive success (RS)	252:288	shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations	252:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
36901705	4	105	from	times	703:707	arg1	average					675:681	average	675:681	average	675:681	On average, FRS was almost two times higher in the anthropogenic than in the natural populations.					
36901705	4	105	from	times	703:707	arg1	populations					757:767	the natural populations	745:767	the natural populations	745:767	On average, FRS was almost two times higher in the anthropogenic than in the natural populations.					
36901705	4	105	from	times	703:707	arg1	anthropogenic					723:735	anthropogenic	723:735	anthropogenic	723:735	On average, FRS was almost two times higher in the anthropogenic than in the natural populations.					
36901705	1	106	theme	composition	237:247	arg1	role					201:204	the role	197:204	the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations	197:376	The purpose of our study was to determine the role of flower structure and nectar composition in shaping the reproductive success (RS) of the generalist orchid Epipactis helleborine in natural and anthropogenic populations.					
35787884	4	0	theme	wastewater	889:898	arg1	performance					910:920	the wastewater treatment performance	885:920	the wastewater treatment performance at various number of L/D cycles and light wavelengths	885:974	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	9	1	theme	oleic	2014:2018	arg1	C18:1					2026:2030	C18:1	2026:2030	C18:1	2026:2030	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	9	1	theme	oleic	2014:2018	arg1	acid					2020:2023	oleic acid	2014:2023	oleic acid (C18:1)	2014:2031	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	9	2	theme	red	2231:2233	arg1	wavelengths					2207:2217	the mixed wavelengths	2197:2217	the mixed wavelengths of blue and red	2197:2233	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	1	3	theme	copious	258:264	arg1	nutrients					266:274	the inherent copious nutrients	245:274	the inherent copious nutrients	245:274	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	7	4	theme	lipid	1746:1750	arg1	productivity					1752:1763	the highest lipid productivity	1734:1763	the highest lipid productivity	1734:1763	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	7	4	theme	lipid	1746:1750	arg1	d					1784:1784	109.0 mg-1 L-1 d	1769:1784	109.0 mg-1 L-1 d	1769:1784	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	8	5	from	methyl	1878:1883	arg1	system					1916:1921	the system	1912:1921	the system	1912:1921	Moreover, gas chromatography analysis demonstrated that the methyl in the range of C16-C18 in the system was higher than 70%.					
35787884	8	5	from	methyl	1878:1883	arg1	range					1892:1896	the range	1888:1896	the range of C16-C18	1888:1907	Moreover, gas chromatography analysis demonstrated that the methyl in the range of C16-C18 in the system was higher than 70%.					
35787884	6	6	theme	L/D	1281:1283	arg1	period					1285:1290	the L/D period	1277:1290	the L/D period	1277:1290	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	4	7	theme	conversion	869:878	arg1	responses					823:831	responses	823:831	responses of the microalgae photo-biochemical conversion	823:878	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	4	7	theme	conversion	869:878	arg1	performance					910:920	the wastewater treatment performance	885:920	the wastewater treatment performance at various number of L/D cycles and light wavelengths	885:974	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	6	8	theme	1.28 g L-1	1206:1215	arg1	higher					1234:1239	higher	1234:1239	higher	1234:1239	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	6	8	theme	1.28 g L-1	1206:1215	arg1	value					1197:1201	a maximum value	1187:1201	a maximum value	1187:1201	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	3	9	theme	photo	702:706	arg1	transfer					717:724	intracellular photo electron transfer	688:724	intracellular photo electron transfer	688:724	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	10	10	theme	peak	2291:2294	arg1	concentration					2296:2308	the peak concentration	2287:2308	the peak concentration of 2.45 g L-1	2287:2322	Finally, Chlorella was cultured in PBR and reached the peak concentration of 2.45 g L-1 by semi-continuous process with the HRT regulation.					
35787884	6	11	theme	control	1258:1264	arg1	group					1266:1270	the control group	1254:1270	the control group	1254:1270	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	3	12	theme	L/D	578:580	arg1	cycles					583:588	light/dark (L/D) cycles	566:588	light/dark (L/D) cycles	566:588	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	4	13	theme	cycles	947:952	arg1	number					933:938	various number	925:938	various number of L/D cycles and light wavelengths	925:974	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	7	14	theme	white	1717:1721	arg1	light					1723:1727	white light	1717:1727	white light	1717:1727	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	8	15	theme	chromatography	1832:1845	arg1	analysis					1847:1854	gas chromatography analysis	1828:1854	gas chromatography analysis	1828:1854	Moreover, gas chromatography analysis demonstrated that the methyl in the range of C16-C18 in the system was higher than 70%.					
35787884	3	16	theme	light/dark	566:575	arg1	cycles					583:588	light/dark (L/D) cycles	566:588	light/dark (L/D) cycles	566:588	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	4	17	theme	light	958:962	arg1	wavelengths					964:974	light wavelengths	958:974	light wavelengths	958:974	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	3	18	theme	microalgae	776:785	arg1	accumulation					795:806	microalgae biomass accumulation	776:806	microalgae biomass accumulation	776:806	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	4	19	theme	various	925:931	arg1	number					933:938	various number	925:938	various number of L/D cycles and light wavelengths	925:974	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	3	20	theme	cycles	583:588	arg1	numbers					555:561	numbers	555:561	numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation	555:806	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	1	21	theme	starch	146:151	arg1	wastewater					153:162	starch wastewater	146:162	starch wastewater (SW)	146:167	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	1	21	theme	starch	146:151	arg1	SW					165:166	SW	165:166	SW	165:166	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	8	22	theme	C16-C18	1901:1907	arg1	range					1892:1896	the range	1888:1896	the range of C16-C18	1888:1907	Moreover, gas chromatography analysis demonstrated that the methyl in the range of C16-C18 in the system was higher than 70%.					
35787884	3	23	theme	wavelength	617:626	arg1	cycles					583:588	light/dark (L/D) cycles	566:588	light/dark (L/D) cycles	566:588	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	3	23	theme	wavelength	617:626	arg1	compositions					595:606	compositions	595:606	compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation	595:806	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	9	24	theme	fatty	2096:2100	arg1	ester					2114:2118	fatty acid methyl ester	2096:2118	fatty acid methyl ester used in biodiesel production	2096:2147	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	10	25	with	process	2343:2349	arg1	regulation					2364:2373	the HRT regulation	2356:2373	the HRT regulation	2356:2373	Finally, Chlorella was cultured in PBR and reached the peak concentration of 2.45 g L-1 by semi-continuous process with the HRT regulation.					
35787884	6	26	theme	red	1547:1549	arg1	light					1551:1555	red light	1547:1555	red light (1.05 g L-1 and 19.3%, respectively)	1547:1592	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	7	27	theme	biomass	1669:1675	arg1	higher					1694:1699	higher	1694:1699	higher	1694:1699	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	7	27	theme	biomass	1669:1675	arg1	productivity					1677:1688	the microalgae biomass productivity	1654:1688	the microalgae biomass productivity	1654:1688	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	9	28	theme	methyl	2107:2112	arg1	ester					2114:2118	fatty acid methyl ester	2096:2118	fatty acid methyl ester used in biodiesel production	2096:2147	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	0	29	theme	photo-regulation	84:99	arg1	strategy					101:108	photo-regulation strategy	84:108	photo-regulation strategy	84:108	Enhanced photoautotrophic growth of Chlorella vulgaris in starch wastewater through photo-regulation strategy.					
35787884	7	30	dep	higher	1694:1699	arg1	ratio					1804:1808	red ratio	1800:1808	red ratio of 2:1	1800:1815	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	5	31	theme	experimental	996:1007	arg1	results					1009:1015	The experimental results	992:1015	The experimental results	992:1015	The experimental results indicated that the biomass production increased when the L/D period was increased from 2 h:2 h-12 h:12 h.					
35787884	9	32	theme	biodiesel	2128:2136	arg1	production					2138:2147	biodiesel production	2128:2147	biodiesel production	2128:2147	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	6	33	theme	monochromatic	1360:1372	arg1	light					1374:1378	monochromatic light	1360:1378	monochromatic light	1360:1378	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	0	34	theme	vulgaris	46:53	arg1	growth					26:31	photoautotrophic growth	9:31	photoautotrophic growth of Chlorella vulgaris	9:53	Enhanced photoautotrophic growth of Chlorella vulgaris in starch wastewater through photo-regulation strategy.					
35787884	2	35	theme	microalgae	453:462	arg1	efficiency					439:448	the poor photosynthetic efficiency	415:448	the poor photosynthetic efficiency of microalgae	415:462	However, the application of this technique is significantly hindered by low biomass production on account of the poor photosynthetic efficiency of microalgae.					
35787884	9	36	theme	acid	1950:1953	arg1	FAMEs					1970:1974	FAMEs	1970:1974	FAMEs	1970:1974	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	9	36	theme	acid	1950:1953	arg1	esters					1962:1967	Fatty acid methyl esters	1944:1967	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1)	1944:2031	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	2	37	theme	biomass	382:388	arg1	production					390:399	low biomass production	378:399	low biomass production	378:399	However, the application of this technique is significantly hindered by low biomass production on account of the poor photosynthetic efficiency of microalgae.					
35787884	1	38	theme	nontoxic	280:287	arg1	compounds					289:297	nontoxic compounds	280:297	nontoxic compounds	280:297	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	1	39	theme	Microalgae	111:120	arg1	biomass					122:128	Microalgae biomass	111:128	Microalgae biomass production with starch wastewater (SW)	111:167	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	6	40	theme	maximum	1385:1391	arg1	1.25 g L-1					1416:1425	1.25 g L-1	1416:1425	1.25 g L-1	1416:1425	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	6	40	theme	maximum	1385:1391	arg1	concentration					1401:1413	the maximum biomass concentration	1381:1413	the maximum biomass concentration (1.25 g L-1)	1381:1426	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	6	40	theme	maximum	1385:1391	arg1	%					1451:1451	32.2%	1447:1451	32.2%	1447:1451	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	2	41	theme	poor	419:422	arg1	efficiency					439:448	the poor photosynthetic efficiency	415:448	the poor photosynthetic efficiency of microalgae	415:462	However, the application of this technique is significantly hindered by low biomass production on account of the poor photosynthetic efficiency of microalgae.					
35787884	0	42	theme	Chlorella	36:44	arg1	vulgaris					46:53	Chlorella vulgaris	36:53	Chlorella vulgaris	36:53	Enhanced photoautotrophic growth of Chlorella vulgaris in starch wastewater through photo-regulation strategy.					
35787884	4	43	theme	microalgae	840:849	arg1	conversion					869:878	the microalgae photo-biochemical conversion	836:878	the microalgae photo-biochemical conversion	836:878	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	2	44	theme	technique	339:347	arg1	application					319:329	the application	315:329	the application of this technique	315:347	However, the application of this technique is significantly hindered by low biomass production on account of the poor photosynthetic efficiency of microalgae.					
35787884	7	45	theme	2:1	1813:1815	arg1	ratio					1804:1808	red ratio	1800:1808	red ratio of 2:1	1800:1815	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	5	46	theme	L/D	1074:1076	arg1	period					1078:1083	the L/D period	1070:1083	the L/D period	1070:1083	The experimental results indicated that the biomass production increased when the L/D period was increased from 2 h:2 h-12 h:12 h.					
35787884	6	47	theme	biomass	1160:1166	arg1	production					1168:1177	the biomass production	1156:1177	the biomass production	1156:1177	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	9	48	contain	containing	1977:1986	arg2	acid					1997:2000	palmitic acid	1988:2000	palmitic acid (C16:0)	1988:2008	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	9	48	contain	containing	1977:1986	arg2	acid					2020:2023	oleic acid	2014:2023	oleic acid (C18:1)	2014:2031	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	9	48	contain	containing	1977:1986	arg1	FAMEs					1970:1974	FAMEs	1970:1974	FAMEs	1970:1974	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	9	48	contain	containing	1977:1986	arg2	C16:0					2003:2007	C16:0	2003:2007	C16:0	2003:2007	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	9	48	contain	containing	1977:1986	arg2	C18:1					2026:2030	C18:1	2026:2030	C18:1	2026:2030	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	9	48	contain	containing	1977:1986	arg1	esters					1962:1967	Fatty acid methyl esters	1944:1967	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1)	1944:2031	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	3	49	theme	effective	655:663	arg1	method					665:670	an effective method	652:670	an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency	652:764	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	3	49	theme	effective	655:663	arg1	wavelength					617:626	light wavelength	611:626	light wavelength	611:626	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	9	50	theme	mixed	2201:2205	arg1	wavelengths					2207:2217	the mixed wavelengths	2197:2217	the mixed wavelengths of blue and red	2197:2233	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	6	51	theme	blue	1487:1490	arg1	light					1492:1496	blue light	1487:1496	blue light	1487:1496	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	7	52	theme	109.0 mg-1 L-1	1769:1782	arg1	productivity					1752:1763	the highest lipid productivity	1734:1763	the highest lipid productivity	1734:1763	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	7	52	theme	109.0 mg-1 L-1	1769:1782	arg1	d					1784:1784	109.0 mg-1 L-1 d	1769:1784	109.0 mg-1 L-1 d	1769:1784	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	3	53	theme	intracellular	688:700	arg1	transfer					717:724	intracellular photo electron transfer	688:724	intracellular photo electron transfer	688:724	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	3	54	theme	photo-regulation	495:510	arg1	strategy					512:519	a photo-regulation strategy	493:519	a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation	493:806	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	6	55	theme	Chlorella	1457:1465	arg1	1.25 g L-1					1416:1425	1.25 g L-1	1416:1425	1.25 g L-1	1416:1425	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	6	55	theme	Chlorella	1457:1465	arg1	concentration					1401:1413	the maximum biomass concentration	1381:1413	the maximum biomass concentration (1.25 g L-1)	1381:1426	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	6	55	theme	Chlorella	1457:1465	arg1	content					1438:1444	lipid content	1432:1444	lipid content	1432:1444	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	6	55	theme	Chlorella	1457:1465	arg1	%					1451:1451	32.2%	1447:1451	32.2%	1447:1451	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	1	56	theme	inherent	249:256	arg1	nutrients					266:274	the inherent copious nutrients	245:274	the inherent copious nutrients	245:274	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	7	57	theme	highest	1738:1744	arg1	productivity					1752:1763	the highest lipid productivity	1734:1763	the highest lipid productivity	1734:1763	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	7	57	theme	highest	1738:1744	arg1	d					1784:1784	109.0 mg-1 L-1 d	1769:1784	109.0 mg-1 L-1 d	1769:1784	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	6	58	theme	L/D	1132:1134	arg1	period					1136:1141	the L/D period	1128:1141	the L/D period	1128:1141	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	4	59	theme	treatment	900:908	arg1	performance					910:920	the wastewater treatment performance	885:920	the wastewater treatment performance at various number of L/D cycles and light wavelengths	885:974	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	1	60	theme	promising	174:182	arg1	approach					184:191	a promising approach	172:191	a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW	172:303	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	1	60	theme	promising	174:182	arg1	production					130:139	Microalgae biomass production	111:139	Microalgae biomass production with starch wastewater (SW)	111:167	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	4	61	theme	photo-biochemical	851:867	arg1	conversion					869:878	the microalgae photo-biochemical conversion	836:878	the microalgae photo-biochemical conversion	836:878	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	3	62	theme	electron	708:715	arg1	transfer					717:724	intracellular photo electron transfer	688:724	intracellular photo electron transfer	688:724	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	8	63	theme	gas	1828:1830	arg1	analysis					1847:1854	gas chromatography analysis	1828:1854	gas chromatography analysis	1828:1854	Moreover, gas chromatography analysis demonstrated that the methyl in the range of C16-C18 in the system was higher than 70%.					
35787884	6	64	theme	minimum	1512:1518	arg1	values					1520:1525	the minimum values	1508:1525	the minimum values	1508:1525	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	1	65	with	production	130:139	arg1	wastewater					153:162	starch wastewater	146:162	starch wastewater (SW)	146:167	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	1	65	with	production	130:139	arg1	SW					165:166	SW	165:166	SW	165:166	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	7	66	theme	red	1604:1606	arg1	light					1608:1612	the red light	1600:1612	the red light	1600:1612	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	6	67	theme	maximum	1189:1195	arg1	higher					1234:1239	higher	1234:1239	higher	1234:1239	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	6	67	theme	maximum	1189:1195	arg1	value					1197:1201	a maximum value	1187:1201	a maximum value	1187:1201	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	10	68	theme	semi-continuous	2327:2341	arg1	process					2343:2349	semi-continuous process	2327:2349	semi-continuous process with the HRT regulation	2327:2373	Finally, Chlorella was cultured in PBR and reached the peak concentration of 2.45 g L-1 by semi-continuous process with the HRT regulation.					
35787884	1	69	theme	waste	204:208	arg1	recovery					210:217	waste recovery	204:217	waste recovery	204:217	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	4	70	theme	L/D	943:945	arg1	cycles					947:952	L/D cycles	943:952	L/D cycles	943:952	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	3	71	theme	photosynthetic	740:753	arg1	efficiency					755:764	photosynthetic efficiency	740:764	photosynthetic efficiency	740:764	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	6	72	theme	microalgae	1336:1345	arg1	growth					1347:1352	microalgae growth	1336:1352	microalgae growth under monochromatic light	1336:1378	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	10	73	theme	HRT	2360:2362	arg1	regulation					2364:2373	the HRT regulation	2356:2373	the HRT regulation	2356:2373	Finally, Chlorella was cultured in PBR and reached the peak concentration of 2.45 g L-1 by semi-continuous process with the HRT regulation.					
35787884	3	74	theme	biomass	787:793	arg1	accumulation					795:806	microalgae biomass accumulation	776:806	microalgae biomass accumulation	776:806	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	1	75	from	reduction	228:236	arg1	SW					302:303	SW	302:303	SW	302:303	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	4	76	from	number	933:938	arg1	responses					823:831	responses	823:831	responses of the microalgae photo-biochemical conversion	823:878	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	4	76	from	number	933:938	arg1	performance					910:920	the wastewater treatment performance	885:920	the wastewater treatment performance at various number of L/D cycles and light wavelengths	885:974	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	3	77	theme	light	611:615	arg1	method					665:670	an effective method	652:670	an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency	652:764	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	3	77	theme	light	611:615	arg1	wavelength					617:626	light wavelength	611:626	light wavelength	611:626	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	3	78	theme	compositions	595:606	arg1	numbers					555:561	numbers	555:561	numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation	555:806	In this regard, we proposed a photo-regulation strategy characterized by the adjusting of numbers of light/dark (L/D) cycles, and compositions of light wavelength, which was proved to be an effective method for stimulating intracellular photo electron transfer and enhancing photosynthetic efficiency, to boost microalgae biomass accumulation.					
35787884	6	79	dep	light	1551:1555	arg1	1.05 g L-1					1558:1567	1.05 g L-1	1558:1567	1.05 g L-1	1558:1567	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	6	79	dep	light	1551:1555	arg1	%					1577:1577	19.3%	1573:1577	19.3%	1573:1577	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	9	80	theme	acid	2102:2105	arg1	ester					2114:2118	fatty acid methyl ester	2096:2118	fatty acid methyl ester used in biodiesel production	2096:2147	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	6	81	theme	biomass	1393:1399	arg1	1.25 g L-1					1416:1425	1.25 g L-1	1416:1425	1.25 g L-1	1416:1425	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	6	81	theme	biomass	1393:1399	arg1	concentration					1401:1413	the maximum biomass concentration	1381:1413	the maximum biomass concentration (1.25 g L-1)	1381:1426	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	6	81	theme	biomass	1393:1399	arg1	%					1451:1451	32.2%	1447:1451	32.2%	1447:1451	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	9	82	theme	ester	2114:2118	arg1	quality					2085:2091	the quality	2081:2091	the quality of fatty acid methyl ester used in biodiesel production	2081:2147	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	7	83	theme	microalgae	1658:1667	arg1	higher					1694:1699	higher	1694:1699	higher	1694:1699	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	7	83	theme	microalgae	1658:1667	arg1	productivity					1677:1688	the microalgae biomass productivity	1654:1688	the microalgae biomass productivity	1654:1688	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	4	84	theme	wavelengths	964:974	arg1	number					933:938	various number	925:938	various number of L/D cycles and light wavelengths	925:974	Additionally, responses of the microalgae photo-biochemical conversion, and the wastewater treatment performance at various number of L/D cycles and light wavelengths were discussed.					
35787884	9	85	theme	Fatty	1944:1948	arg1	FAMEs					1970:1974	FAMEs	1970:1974	FAMEs	1970:1974	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	9	85	theme	Fatty	1944:1948	arg1	esters					1962:1967	Fatty acid methyl esters	1944:1967	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1)	1944:2031	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	0	86	theme	photoautotrophic	9:24	arg1	growth					26:31	photoautotrophic growth	9:31	photoautotrophic growth of Chlorella vulgaris	9:53	Enhanced photoautotrophic growth of Chlorella vulgaris in starch wastewater through photo-regulation strategy.					
35787884	9	87	theme	methyl	1955:1960	arg1	FAMEs					1970:1974	FAMEs	1970:1974	FAMEs	1970:1974	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	9	87	theme	methyl	1955:1960	arg1	esters					1962:1967	Fatty acid methyl esters	1944:1967	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1)	1944:2031	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	2	88	theme	low	378:380	arg1	production					390:399	low biomass production	378:399	low biomass production	378:399	However, the application of this technique is significantly hindered by low biomass production on account of the poor photosynthetic efficiency of microalgae.					
35787884	1	89	theme	biomass	122:128	arg1	approach					184:191	a promising approach	172:191	a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW	172:303	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	1	89	theme	biomass	122:128	arg1	production					130:139	Microalgae biomass production	111:139	Microalgae biomass production with starch wastewater (SW)	111:167	Microalgae biomass production with starch wastewater (SW) is a promising approach to realize waste recovery and cost reduction due to the inherent copious nutrients and nontoxic compounds in SW.					
35787884	2	90	theme	photosynthetic	424:437	arg1	efficiency					439:448	the poor photosynthetic efficiency	415:448	the poor photosynthetic efficiency of microalgae	415:462	However, the application of this technique is significantly hindered by low biomass production on account of the poor photosynthetic efficiency of microalgae.					
35787884	6	91	theme	lipid	1432:1436	arg1	content					1438:1444	lipid content	1432:1444	lipid content	1432:1444	When the L/D period was 2 h:2 h, the biomass production reached a maximum value of 1.28 g L-1, which was 19.6% higher than that of the control group when the L/D period was 12 h:12 h. Furthermore, with respect to microalgae growth under monochromatic light, the maximum biomass concentration (1.25 g L-1) and lipid content (32.2%) of Chlorella were achieved under blue light; whereas, the minimum values were attained under red light (1.05 g L-1 and 19.3%, respectively).					
35787884	7	92	theme	blue	1618:1621	arg1	light					1623:1627	blue light	1618:1627	blue light	1618:1627	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	10	93	theme	2.45 g L-1	2313:2322	arg1	concentration					2296:2308	the peak concentration	2287:2308	the peak concentration of 2.45 g L-1	2287:2322	Finally, Chlorella was cultured in PBR and reached the peak concentration of 2.45 g L-1 by semi-continuous process with the HRT regulation.					
35787884	0	94	theme	starch	58:63	arg1	wastewater					65:74	starch wastewater	58:74	starch wastewater	58:74	Enhanced photoautotrophic growth of Chlorella vulgaris in starch wastewater through photo-regulation strategy.					
35787884	9	95	theme	biodiesel	2066:2074	arg1	production					2052:2061	production	2052:2061	production of biodiesel	2052:2074	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	9	96	theme	palmitic	1988:1995	arg1	acid					1997:2000	palmitic acid	1988:2000	palmitic acid (C16:0)	1988:2008	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	9	96	theme	palmitic	1988:1995	arg1	C16:0					2003:2007	C16:0	2003:2007	C16:0	2003:2007	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
35787884	7	97	theme	red	1800:1802	arg1	ratio					1804:1808	red ratio	1800:1808	red ratio of 2:1	1800:1815	When the red light and blue light were mixed and supplied, the microalgae biomass productivity was higher than that under white light, and the highest lipid productivity was 109.0 mg-1 L-1 d under a blue: red ratio of 2:1.					
35787884	5	98	theme	biomass	1036:1042	arg1	production					1044:1053	the biomass production	1032:1053	the biomass production	1032:1053	The experimental results indicated that the biomass production increased when the L/D period was increased from 2 h:2 h-12 h:12 h.					
35787884	9	99	theme	blue	2222:2225	arg1	wavelengths					2207:2217	the mixed wavelengths	2197:2217	the mixed wavelengths of blue and red	2197:2233	Fatty acid methyl esters (FAMEs) containing palmitic acid (C16:0) and oleic acid (C18:1) are beneficial for production of biodiesel, and the quality of fatty acid methyl ester used in biodiesel production can be improved using microalgae cultured under the mixed wavelengths of blue and red.					
36717990	6	0	theme	DD	1074:1075	arg1	treatment					1077:1085	DD treatment	1074:1085	DD treatment	1074:1085	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	6	1	from	mechanism	1054:1062	arg1	treatment					1077:1085	DD treatment	1074:1085	DD treatment	1074:1085	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	11	2	theme	significant	1919:1929	arg1	increase					1931:1938	a significant increase	1917:1938	a significant increase in sucrose preference	1917:1960	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	5	3	theme	Depressed	653:661	arg1	mice					663:666	Depressed mice	653:666	Depressed mice	653:666	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	14	4	theme	potential	2297:2305	arg1	mechanism					2307:2315	its potential mechanism	2293:2315	its potential mechanism involving multiple compounds	2293:2344	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	5	5	theme	antidepressant	755:768	arg1	effects					770:776	the antidepressant effects	751:776	the antidepressant effects of PMC	751:783	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	0	6	theme	Orbitrap	225:232	arg1	Spectrometer					239:250	Quadrupole Exactive Orbitrap Mass Spectrometer	205:250	Quadrupole Exactive Orbitrap Mass Spectrometer	205:250	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	6	7	from	targets	1031:1037	arg1	treatment					1077:1085	DD treatment	1074:1085	DD treatment	1074:1085	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	3	8	theme	PMC	536:538	arg1	effects					510:516	the antidepressant effects	491:516	the antidepressant effects	491:516	Here, the antidepressant effects and mechanisms of PMC were explored for the first time.					
36717990	3	8	theme	PMC	536:538	arg1	mechanisms					522:531	mechanisms	522:531	mechanisms	522:531	Here, the antidepressant effects and mechanisms of PMC were explored for the first time.					
36717990	4	9	from	dose	647:650	arg1	safety					598:603	the safety	594:603	the safety of PMC at a 10-fold clinically equivalent dose	594:650	METHODS We observed the safety of PMC at a 10-fold clinically equivalent dose.					
36717990	5	10	theme	suspension	830:839	arg1	test					841:844	the tail suspension test	821:844	the tail suspension test	821:844	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	8	11	from	effects	1421:1427	arg1	evaluation					1388:1397	the safety evaluation	1377:1397	the safety evaluation of PMC	1377:1404	RESULTS In the safety evaluation of PMC, no toxic side effects or deaths occurred.					
36717990	6	12	theme	exactive	957:964	arg1	spectrometer					980:991	quadrupole exactive orbitrap mass spectrometer	946:991	quadrupole exactive orbitrap mass spectrometer	946:991	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	14	13	theme	CONCLUSION	2221:2230	arg1	PMC					2232:2234	CONCLUSION PMC	2221:2234	CONCLUSION PMC	2221:2234	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	14	13	theme	CONCLUSION	2221:2230	arg1	medicine					2279:2286	a relatively safe antidepressant herbal medicine	2239:2286	a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways	2239:2367	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	5	14	theme	unpredictable	692:704	arg1	CUMS					719:722	CUMS	719:722	CUMS	719:722	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	5	14	theme	unpredictable	692:704	arg1	stress					711:716	chronic unpredictable mild stress (CUMS)	684:723	chronic unpredictable mild stress (CUMS)	684:723	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	10	15	theme	model	1641:1645	arg1	group					1647:1651	the model group	1637:1651	the model group of mice	1637:1659	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	10	15	theme	model	1641:1645	arg1	mice					1656:1659	mice	1656:1659	mice	1656:1659	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	7	16	theme	network	1321:1327	arg1	construction					1329:1340	PMC-compound-target-pathway-DD network construction	1290:1340	PMC-compound-target-pathway-DD network construction	1290:1340	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	16	theme	network	1321:1327	arg1	compounds					1158:1166	active compounds	1151:1166	active compounds	1151:1166	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	4	17	dep	METHODS	574:580	arg1	observed					585:592	observed	585:592	observed the safety of PMC at a 10-fold clinically equivalent dose	585:650	METHODS We observed the safety of PMC at a 10-fold clinically equivalent dose.					
36717990	9	18	theme	significant	1463:1473	arg1	TP					1520:1521	TP	1520:1521	TP	1520:1521	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	9	18	theme	significant	1463:1473	arg1	ALT					1506:1508	ALT	1506:1508	ALT	1506:1508	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	9	18	theme	significant	1463:1473	arg1	differences					1475:1485	no significant differences	1460:1485	no significant differences in liver function (ALT, AST, and TP; P > 0.05)	1460:1532	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	9	18	theme	significant	1463:1473	arg1	AST					1511:1513	AST	1511:1513	AST	1511:1513	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	0	19	theme	Ultra-High	147:156	arg1	Chromatography					177:190	Ultra-High Performance Liquid Chromatography	147:190	Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer	147:250	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	6	20	theme	performance	899:909	arg1	chromatography					918:931	ultra-high performance liquid chromatography	888:931	ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer	888:991	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	11	21	theme	immobility	1897:1906	arg1	time					1908:1911	immobility time	1897:1911	immobility time	1897:1911	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	0	22	theme	Liquid	170:175	arg1	Chromatography					177:190	Ultra-High Performance Liquid Chromatography	147:190	Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer	147:250	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	11	23	theme	low	1830:1832	arg1	PMC					1826:1828	the PMC low, medium, and high dose groups	1822:1862	PMC	1826:1828	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	0	24	from	Disorder	91:98	arg1	Mice					103:106	Mice	103:106	Mice	103:106	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	0	25	theme	Caulis	60:65	arg1	Mechanism					27:35	the Mechanism	23:35	the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer	23:250	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	7	26	dep	compounds	1158:1166	arg1	construction					1329:1340	PMC-compound-target-pathway-DD network construction	1290:1340	PMC-compound-target-pathway-DD network construction	1290:1340	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	26	dep	compounds	1158:1166	arg1	compounds					1158:1166	active compounds	1151:1166	active compounds	1151:1166	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	26	dep	compounds	1158:1166	arg1	Encyclopedia					1237:1248	Encyclopedia	1237:1248	Encyclopedia	1237:1248	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	26	dep	compounds	1158:1166	arg1	analysis					1221:1228	Gene Ontology (GO) analysis	1202:1228	Gene Ontology (GO) analysis	1202:1228	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	26	dep	compounds	1158:1166	arg1	docking					1357:1363	Molecular docking	1347:1363	Molecular docking	1347:1363	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	26	dep	compounds	1158:1166	arg1	screening					1191:1199	screening	1191:1199	screening	1191:1199	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	0	27	theme	Quadrupole	205:214	arg1	Spectrometer					239:250	Quadrupole Exactive Orbitrap Mass Spectrometer	205:250	Quadrupole Exactive Orbitrap Mass Spectrometer	205:250	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	12	28	theme	DD	2119:2120	arg1	treatment					2106:2114	the treatment	2102:2114	the treatment of DD	2102:2120	In the PMC-Compound-Target-Pathway-DD network, 54 active compounds, 83 common targets, and 13 major signaling pathways were identified for the treatment of DD.					
36717990	14	29	with	medicine	2279:2286	arg1	mechanism					2307:2315	its potential mechanism	2293:2315	its potential mechanism involving multiple compounds	2293:2344	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	14	29	with	medicine	2279:2286	arg1	targets					2347:2353	targets	2347:2353	targets	2347:2353	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	14	29	with	medicine	2279:2286	arg1	pathways					2360:2367	pathways	2360:2367	pathways	2360:2367	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	12	30	theme	major	2057:2061	arg1	pathways					2073:2080	13 major signaling pathways	2054:2080	13 major signaling pathways	2054:2080	In the PMC-Compound-Target-Pathway-DD network, 54 active compounds, 83 common targets, and 13 major signaling pathways were identified for the treatment of DD.					
36717990	11	31	from	decrease	1885:1892	arg1	time					1908:1911	immobility time	1897:1911	immobility time	1897:1911	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	11	31	from	decrease	1885:1892	arg1	preference					1951:1960	sucrose preference	1943:1960	sucrose preference	1943:1960	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	7	32	theme	Ontology	1207:1214	arg1	compounds					1158:1166	active compounds	1151:1166	active compounds	1151:1166	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	32	theme	Ontology	1207:1214	arg1	analysis					1221:1228	Gene Ontology (GO) analysis	1202:1228	Gene Ontology (GO) analysis	1202:1228	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	6	33	theme	mass	975:978	arg1	spectrometer					980:991	quadrupole exactive orbitrap mass spectrometer	946:991	quadrupole exactive orbitrap mass spectrometer	946:991	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	9	34	dep	differences	1475:1485	arg1	>					1575:1575	>	1575:1575	>	1575:1575	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	2	35	theme	traditional	389:399	arg1	medicine					409:416	a traditional Chinese medicine	387:416	a traditional Chinese medicine	387:416	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	2	35	theme	traditional	389:399	arg1	Caulis					373:378	Polygoni Multiflori Caulis	353:378	Polygoni Multiflori Caulis (PMC)	353:384	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	0	36	theme	Systematic	0:9	arg1	Analysis					11:18	Systematic Analysis	0:18	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.	0:251	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	9	37	from	function	1545:1552	arg1	function					1496:1503	liver function	1490:1503	liver function	1490:1503	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	9	37	from	function	1545:1552	arg1	mice					1600:1603	mice	1600:1603	mice	1600:1603	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	9	37	from	function	1545:1552	arg1	group					1591:1595	each group	1586:1595	each group of mice	1586:1603	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	6	38	theme	potential	1044:1052	arg1	mechanism					1054:1062	potential mechanism	1044:1062	potential mechanism	1044:1062	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	8	39	theme	safety	1381:1386	arg1	evaluation					1388:1397	the safety evaluation	1377:1397	the safety evaluation of PMC	1377:1404	RESULTS In the safety evaluation of PMC, no toxic side effects or deaths occurred.					
36717990	5	40	theme	sucrose	793:799	arg1	test					812:815	the sucrose preference test	789:815	the sucrose preference test	789:815	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	1	41	theme	common	308:313	arg1	BACKGROUND					253:262	BACKGROUND	253:262	BACKGROUND	253:262	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	1	41	theme	common	308:313	arg1	disorder					289:296	OBJECTIVE Depressive disorder	268:296	OBJECTIVE Depressive disorder (DD)	268:301	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	1	41	theme	common	308:313	arg1	disease					344:350	a common chronic and highly disabling disease	306:350	a common chronic and highly disabling disease	306:350	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	2	42	theme	Pharmacopoeia	470:482	arg1	edition					447:453	the 2020 edition	438:453	the 2020 edition of the Chinese Pharmacopoeia	438:482	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	7	43	theme	active	1151:1156	arg1	construction					1329:1340	PMC-compound-target-pathway-DD network construction	1290:1340	PMC-compound-target-pathway-DD network construction	1290:1340	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	43	theme	active	1151:1156	arg1	compounds					1158:1166	active compounds	1151:1166	active compounds	1151:1166	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	43	theme	active	1151:1156	arg1	Encyclopedia					1237:1248	Encyclopedia	1237:1248	Encyclopedia	1237:1248	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	43	theme	active	1151:1156	arg1	analysis					1221:1228	Gene Ontology (GO) analysis	1202:1228	Gene Ontology (GO) analysis	1202:1228	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	43	theme	active	1151:1156	arg1	docking					1357:1363	Molecular docking	1347:1363	Molecular docking	1347:1363	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	43	theme	active	1151:1156	arg1	screening					1191:1199	screening	1191:1199	screening	1191:1199	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	0	44	theme	Mechanism	27:35	arg1	Analysis					11:18	Systematic Analysis	0:18	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.	0:251	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	9	45	theme	kidney	1538:1543	arg1	function					1545:1552	kidney function	1538:1552	kidney function	1538:1552	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	6	46	theme	important	1021:1029	arg1	targets					1031:1037	important targets	1021:1037	important targets	1021:1037	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	10	47	theme	immobility	1739:1748	arg1	time					1750:1753	immobility time	1739:1753	immobility time	1739:1753	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	14	48	theme	herbal	2272:2277	arg1	PMC					2232:2234	CONCLUSION PMC	2221:2234	CONCLUSION PMC	2221:2234	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	14	48	theme	herbal	2272:2277	arg1	medicine					2279:2286	a relatively safe antidepressant herbal medicine	2239:2286	a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways	2239:2367	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	1	49	theme	disabling	334:342	arg1	BACKGROUND					253:262	BACKGROUND	253:262	BACKGROUND	253:262	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	1	49	theme	disabling	334:342	arg1	disorder					289:296	OBJECTIVE Depressive disorder	268:296	OBJECTIVE Depressive disorder (DD)	268:301	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	1	49	theme	disabling	334:342	arg1	disease					344:350	a common chronic and highly disabling disease	306:350	a common chronic and highly disabling disease	306:350	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	6	50	theme	network	1106:1112	arg1	pharmacology					1114:1125	network pharmacology	1106:1125	network pharmacology	1106:1125	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	10	51	theme	or	1765:1766	arg1	<					1770:1770	P < 0.01 or P < 0.05	1756:1775	P < 0.01 or P < 0.05	1756:1775	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	8	52	theme	toxic	1410:1414	arg1	effects					1421:1427	no toxic side effects	1407:1427	no toxic side effects	1407:1427	RESULTS In the safety evaluation of PMC, no toxic side effects or deaths occurred.					
36717990	14	53	theme	safe	2252:2255	arg1	PMC					2232:2234	CONCLUSION PMC	2221:2234	CONCLUSION PMC	2221:2234	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	14	53	theme	safe	2252:2255	arg1	medicine					2279:2286	a relatively safe antidepressant herbal medicine	2239:2286	a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways	2239:2367	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	2	54	theme	Polygoni	353:360	arg1	PMC					381:383	PMC	381:383	PMC	381:383	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	2	54	theme	Polygoni	353:360	arg1	Caulis					373:378	Polygoni Multiflori Caulis	353:378	Polygoni Multiflori Caulis (PMC)	353:384	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	2	54	theme	Polygoni	353:360	arg1	medicine					409:416	a traditional Chinese medicine	387:416	a traditional Chinese medicine	387:416	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	10	55	theme	control	1622:1628	arg1	group					1630:1634	the control group	1618:1634	the control group	1618:1634	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	10	56	theme	<	1758:1758	arg1	<					1770:1770	P < 0.01 or P < 0.05	1756:1775	P < 0.01 or P < 0.05	1756:1775	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	13	57	theme	active	2159:2164	arg1	compounds					2166:2174	the active compounds	2155:2174	the active compounds	2155:2174	Molecular docking verified that the active compounds could effectively bind with the hub targets.					
36717990	1	58	theme	OBJECTIVE	268:276	arg1	BACKGROUND					253:262	BACKGROUND	253:262	BACKGROUND	253:262	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	1	58	theme	OBJECTIVE	268:276	arg1	DD					299:300	DD	299:300	DD	299:300	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	1	58	theme	OBJECTIVE	268:276	arg1	disorder					289:296	OBJECTIVE Depressive disorder	268:296	OBJECTIVE Depressive disorder (DD)	268:301	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	1	58	theme	OBJECTIVE	268:276	arg1	disease					344:350	a common chronic and highly disabling disease	306:350	a common chronic and highly disabling disease	306:350	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	6	59	theme	ultra-high	888:897	arg1	chromatography					918:931	ultra-high performance liquid chromatography	888:931	ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer	888:991	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	8	60	from	deaths	1432:1437	arg1	evaluation					1388:1397	the safety evaluation	1377:1397	the safety evaluation of PMC	1377:1404	RESULTS In the safety evaluation of PMC, no toxic side effects or deaths occurred.					
36717990	3	61	theme	antidepressant	495:508	arg1	effects					510:516	the antidepressant effects	491:516	the antidepressant effects	491:516	Here, the antidepressant effects and mechanisms of PMC were explored for the first time.					
36717990	14	62	theme	multiple	2327:2334	arg1	compounds					2336:2344	multiple compounds	2327:2344	multiple compounds	2327:2344	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	11	63	theme	sucrose	1943:1949	arg1	preference					1951:1960	sucrose preference	1943:1960	sucrose preference	1943:1960	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	0	64	theme	Exactive	216:223	arg1	Spectrometer					239:250	Quadrupole Exactive Orbitrap Mass Spectrometer	205:250	Quadrupole Exactive Orbitrap Mass Spectrometer	205:250	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	8	65	from	RESULTS	1366:1372	arg1	evaluation					1388:1397	the safety evaluation	1377:1397	the safety evaluation of PMC	1377:1404	RESULTS In the safety evaluation of PMC, no toxic side effects or deaths occurred.					
36717990	10	66	theme	mice	1656:1659	arg1	group					1647:1651	the model group	1637:1651	the model group of mice	1637:1659	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	10	66	theme	mice	1656:1659	arg1	mice					1656:1659	mice	1656:1659	mice	1656:1659	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	6	67	theme	PMC	866:868	arg1	composition					851:861	The composition	847:861	The composition of PMC	847:868	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	0	68	theme	Mass	234:237	arg1	Spectrometer					239:250	Quadrupole Exactive Orbitrap Mass Spectrometer	205:250	Quadrupole Exactive Orbitrap Mass Spectrometer	205:250	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	5	69	theme	tail	825:828	arg1	test					841:844	the tail suspension test	821:844	the tail suspension test	821:844	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	4	70	theme	equivalent	636:645	arg1	dose					647:650	a 10-fold clinically equivalent dose	615:650	a 10-fold clinically equivalent dose	615:650	METHODS We observed the safety of PMC at a 10-fold clinically equivalent dose.					
36717990	3	71	theme	first	562:566	arg1	time					568:571	the first time	558:571	the first time	558:571	Here, the antidepressant effects and mechanisms of PMC were explored for the first time.					
36717990	6	72	theme	orbitrap	966:973	arg1	spectrometer					980:991	quadrupole exactive orbitrap mass spectrometer	946:991	quadrupole exactive orbitrap mass spectrometer	946:991	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	9	73	from	differences	1475:1485	arg1	function					1496:1503	liver function	1490:1503	liver function	1490:1503	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	9	73	from	differences	1475:1485	arg1	mice					1600:1603	mice	1600:1603	mice	1600:1603	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	9	73	from	differences	1475:1485	arg1	group					1591:1595	each group	1586:1595	each group of mice	1586:1603	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	5	74	theme	chronic	684:690	arg1	CUMS					719:722	CUMS	719:722	CUMS	719:722	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	5	74	theme	chronic	684:690	arg1	stress					711:716	chronic unpredictable mild stress (CUMS)	684:723	chronic unpredictable mild stress (CUMS)	684:723	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	11	75	theme	high	1847:1850	arg1	groups					1857:1862	high dose groups	1847:1862	high dose groups	1847:1862	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	5	76	theme	preference	801:810	arg1	test					812:815	the sucrose preference test	789:815	the sucrose preference test	789:815	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	6	77	theme	quadrupole	946:955	arg1	spectrometer					980:991	quadrupole exactive orbitrap mass spectrometer	946:991	quadrupole exactive orbitrap mass spectrometer	946:991	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	10	78	dep	showed	1661:1666	arg1	decreased					1682:1690	decreased	1682:1690	decreased sucrose preference	1682:1709	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	10	78	dep	showed	1661:1666	arg1	increased					1729:1737	increased	1729:1737	significantly increased immobility time	1715:1753	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	5	79	theme	mild	706:709	arg1	CUMS					719:722	CUMS	719:722	CUMS	719:722	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	5	79	theme	mild	706:709	arg1	stress					711:716	chronic unpredictable mild stress (CUMS)	684:723	chronic unpredictable mild stress (CUMS)	684:723	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	10	80	theme	sucrose	1692:1698	arg1	preference					1700:1709	sucrose preference	1692:1709	sucrose preference	1692:1709	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	9	81	theme	mice	1600:1603	arg1	mice					1600:1603	mice	1600:1603	mice	1600:1603	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	9	81	theme	mice	1600:1603	arg1	group					1591:1595	each group	1586:1595	each group of mice	1586:1603	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	10	82	dep	decreased	1682:1690	arg1	<					1770:1770	P < 0.01 or P < 0.05	1756:1775	P < 0.01 or P < 0.05	1756:1775	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	11	83	from	increase	1931:1938	arg1	time					1908:1911	immobility time	1897:1911	immobility time	1897:1911	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	11	83	from	increase	1931:1938	arg1	preference					1951:1960	sucrose preference	1943:1960	sucrose preference	1943:1960	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	0	84	theme	Performance	158:168	arg1	Chromatography					177:190	Ultra-High Performance Liquid Chromatography	147:190	Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer	147:250	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	6	85	theme	liquid	911:916	arg1	chromatography					918:931	ultra-high performance liquid chromatography	888:931	ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer	888:991	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	9	86	theme	liver	1490:1494	arg1	function					1496:1503	liver function	1490:1503	liver function	1490:1503	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	13	87	theme	Molecular	2123:2131	arg1	docking					2133:2139	Molecular docking	2123:2139	Molecular docking	2123:2139	Molecular docking verified that the active compounds could effectively bind with the hub targets.					
36717990	6	88	theme	active	1002:1007	arg1	components					1009:1018	the active components	998:1018	the active components	998:1018	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	1	89	dep	common	308:313	arg1	chronic					315:321	chronic	315:321	chronic	315:321	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	7	90	theme	Molecular	1347:1355	arg1	docking					1357:1363	Molecular docking	1347:1363	Molecular docking	1347:1363	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	90	theme	Molecular	1347:1355	arg1	compounds					1158:1166	active compounds	1151:1166	active compounds	1151:1166	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	0	91	theme	Depressive	80:89	arg1	Disorder					91:98	Depressive Disorder	80:98	Depressive Disorder in Mice	80:106	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	0	92	theme	Network	112:118	arg1	Pharmacology					120:131	Network Pharmacology	112:131	Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer	112:250	Systematic Analysis of the Mechanism of Polygoni Multiflori Caulis in Improving Depressive Disorder in Mice via Network Pharmacology Combined with Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Exactive Orbitrap Mass Spectrometer.					
36717990	7	93	theme	Gene	1202:1205	arg1	Ontology					1207:1214	Gene Ontology	1202:1214	Gene Ontology (GO) analysis	1202:1228	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	11	94	theme	dose	1852:1855	arg1	groups					1857:1862	high dose groups	1847:1862	high dose groups	1847:1862	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	6	95	from	components	1009:1018	arg1	treatment					1077:1085	DD treatment	1074:1085	DD treatment	1074:1085	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	6	96	theme	PMC	1067:1069	arg1	mechanism					1054:1062	potential mechanism	1044:1062	potential mechanism	1044:1062	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	6	96	theme	PMC	1067:1069	arg1	components					1009:1018	the active components	998:1018	the active components	998:1018	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	6	96	theme	PMC	1067:1069	arg1	targets					1031:1037	important targets	1021:1037	important targets	1021:1037	The composition of PMC was identified by ultra-high performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometer, and the active components, important targets, and potential mechanism of PMC in DD treatment were predicted via network pharmacology.					
36717990	12	97	theme	signaling	2063:2071	arg1	pathways					2073:2080	13 major signaling pathways	2054:2080	13 major signaling pathways	2054:2080	In the PMC-Compound-Target-Pathway-DD network, 54 active compounds, 83 common targets, and 13 major signaling pathways were identified for the treatment of DD.					
36717990	2	98	theme	Chinese	401:407	arg1	medicine					409:416	a traditional Chinese medicine	387:416	a traditional Chinese medicine	387:416	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	2	98	theme	Chinese	401:407	arg1	Caulis					373:378	Polygoni Multiflori Caulis	353:378	Polygoni Multiflori Caulis (PMC)	353:384	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	5	99	theme	PMC	781:783	arg1	effects					770:776	the antidepressant effects	751:776	the antidepressant effects of PMC	751:783	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	5	100	used	used	734:737	arg2	mice					663:666	Depressed mice	653:666	Depressed mice	653:666	Depressed mice were induced by chronic unpredictable mild stress (CUMS) and were used to evaluate the antidepressant effects of PMC via the sucrose preference test and the tail suspension test.					
36717990	11	101	from	mice	1814:1817	arg1	PMC					1826:1828	the PMC low, medium, and high dose groups	1822:1862	PMC	1826:1828	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	11	101	from	mice	1814:1817	arg1	groups					1857:1862	high dose groups	1847:1862	high dose groups	1847:1862	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	11	101	from	mice	1814:1817	arg1	medium					1835:1840	medium	1835:1840	medium	1835:1840	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	9	102	theme	P	1524:1524	arg1	>					1526:1526	P > 0.05	1524:1531	P > 0.05	1524:1531	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	7	103	theme	PMC-compound-target-pathway-DD	1290:1319	arg1	construction					1329:1340	PMC-compound-target-pathway-DD network construction	1290:1340	PMC-compound-target-pathway-DD network construction	1290:1340	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	103	theme	PMC-compound-target-pathway-DD	1290:1319	arg1	compounds					1158:1166	active compounds	1151:1166	active compounds	1151:1166	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	2	104	theme	2020	442:445	arg1	edition					447:453	the 2020 edition	438:453	the 2020 edition of the Chinese Pharmacopoeia	438:482	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	12	105	theme	common	2034:2039	arg1	targets					2041:2047	83 common targets	2031:2047	83 common targets	2031:2047	In the PMC-Compound-Target-Pathway-DD network, 54 active compounds, 83 common targets, and 13 major signaling pathways were identified for the treatment of DD.					
36717990	7	106	theme	annotation	1278:1287	arg1	docking					1357:1363	Molecular docking	1347:1363	Molecular docking	1347:1363	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	106	theme	annotation	1278:1287	arg1	screening					1191:1199	screening	1191:1199	screening	1191:1199	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	106	theme	annotation	1278:1287	arg1	construction					1329:1340	PMC-compound-target-pathway-DD network construction	1290:1340	PMC-compound-target-pathway-DD network construction	1290:1340	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	106	theme	annotation	1278:1287	arg1	compounds					1158:1166	active compounds	1151:1166	active compounds	1151:1166	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	106	theme	annotation	1278:1287	arg1	analysis					1221:1228	Gene Ontology (GO) analysis	1202:1228	Gene Ontology (GO) analysis	1202:1228	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	10	107	theme	P	1756:1756	arg1	<					1770:1770	P < 0.01 or P < 0.05	1756:1775	P < 0.01 or P < 0.05	1756:1775	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	9	108	dep	ALT	1506:1508	arg1	>					1526:1526	P > 0.05	1524:1531	P > 0.05	1524:1531	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	11	109	theme	model	1797:1801	arg1	group					1803:1807	the model group	1793:1807	the model group	1793:1807	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	13	110	theme	hub	2208:2210	arg1	targets					2212:2218	the hub targets	2204:2218	the hub targets	2204:2218	Molecular docking verified that the active compounds could effectively bind with the hub targets.					
36717990	2	111	theme	Chinese	462:468	arg1	Pharmacopoeia					470:482	the Chinese Pharmacopoeia	458:482	the Chinese Pharmacopoeia	458:482	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	12	112	theme	active	2013:2018	arg1	compounds					2020:2028	54 active compounds	2010:2028	54 active compounds	2010:2028	In the PMC-Compound-Target-Pathway-DD network, 54 active compounds, 83 common targets, and 13 major signaling pathways were identified for the treatment of DD.					
36717990	9	113	dep	>	1575:1575	arg1	CRE					1560:1562	CRE	1560:1562	CRE	1560:1562	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	9	113	dep	>	1575:1575	arg1	UA					1569:1570	UA	1569:1570	UA	1569:1570	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	9	113	dep	>	1575:1575	arg1	BUN					1555:1557	BUN	1555:1557	BUN	1555:1557	There were no significant differences in liver function (ALT, AST, and TP; P > 0.05) and kidney function (BUN, CRE, and UA; P > 0.05) in each group of mice.					
36717990	7	114	theme	Genomes	1263:1269	arg1	annotation					1278:1287	Genes and Genomes (KEGG) annotation	1253:1287	Genes and Genomes (KEGG) annotation	1253:1287	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	11	115	theme	significant	1873:1883	arg1	decrease					1885:1892	a significant decrease	1871:1892	a significant decrease in immobility time	1871:1911	Compared with the model group, the mice in the PMC low, medium, and high dose groups showed a significant decrease in immobility time and a significant increase in sucrose preference.					
36717990	10	116	theme	P	1768:1768	arg1	<					1770:1770	P < 0.01 or P < 0.05	1756:1775	P < 0.01 or P < 0.05	1756:1775	Compared to the control group, the model group of mice showed significantly decreased sucrose preference and significantly increased immobility time (P < 0.01 or P < 0.05).					
36717990	8	117	theme	PMC	1402:1404	arg1	evaluation					1388:1397	the safety evaluation	1377:1397	the safety evaluation of PMC	1377:1404	RESULTS In the safety evaluation of PMC, no toxic side effects or deaths occurred.					
36717990	14	118	theme	antidepressant	2257:2270	arg1	PMC					2232:2234	CONCLUSION PMC	2221:2234	CONCLUSION PMC	2221:2234	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	14	118	theme	antidepressant	2257:2270	arg1	medicine					2279:2286	a relatively safe antidepressant herbal medicine	2239:2286	a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways	2239:2367	CONCLUSION PMC is a relatively safe antidepressant herbal medicine with its potential mechanism involving multiple compounds, targets, and pathways.					
36717990	2	119	theme	Multiflori	362:371	arg1	PMC					381:383	PMC	381:383	PMC	381:383	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	2	119	theme	Multiflori	362:371	arg1	Caulis					373:378	Polygoni Multiflori Caulis	353:378	Polygoni Multiflori Caulis (PMC)	353:384	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	2	119	theme	Multiflori	362:371	arg1	medicine					409:416	a traditional Chinese medicine	387:416	a traditional Chinese medicine	387:416	Polygoni Multiflori Caulis (PMC), a traditional Chinese medicine, has been listed in the 2020 edition of the Chinese Pharmacopoeia.					
36717990	7	120	theme	Genes	1253:1257	arg1	annotation					1278:1287	Genes and Genomes (KEGG) annotation	1253:1287	Genes and Genomes (KEGG) annotation	1253:1287	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	7	121	dep	Ontology	1207:1214	arg1	GO					1217:1218	GO	1217:1218	GO	1217:1218	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	12	122	theme	PMC-Compound-Target-Pathway-DD	1970:1999	arg1	network					2001:2007	the PMC-Compound-Target-Pathway-DD network	1966:2007	the PMC-Compound-Target-Pathway-DD network	1966:2007	In the PMC-Compound-Target-Pathway-DD network, 54 active compounds, 83 common targets, and 13 major signaling pathways were identified for the treatment of DD.					
36717990	4	123	theme	PMC	608:610	arg1	safety					598:603	the safety	594:603	the safety of PMC at a 10-fold clinically equivalent dose	594:650	METHODS We observed the safety of PMC at a 10-fold clinically equivalent dose.					
36717990	8	124	theme	side	1416:1419	arg1	effects					1421:1427	no toxic side effects	1407:1427	no toxic side effects	1407:1427	RESULTS In the safety evaluation of PMC, no toxic side effects or deaths occurred.					
36717990	7	125	theme	DD-related	1172:1181	arg1	targets					1183:1189	DD-related targets	1172:1189	DD-related targets	1172:1189	Investigation included active compounds and DD-related targets screening, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, PMC-compound-target-pathway-DD network construction, and Molecular docking.					
36717990	1	126	theme	Depressive	278:287	arg1	BACKGROUND					253:262	BACKGROUND	253:262	BACKGROUND	253:262	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	1	126	theme	Depressive	278:287	arg1	DD					299:300	DD	299:300	DD	299:300	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	1	126	theme	Depressive	278:287	arg1	disorder					289:296	OBJECTIVE Depressive disorder	268:296	OBJECTIVE Depressive disorder (DD)	268:301	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
36717990	1	126	theme	Depressive	278:287	arg1	disease					344:350	a common chronic and highly disabling disease	306:350	a common chronic and highly disabling disease	306:350	BACKGROUND AND OBJECTIVE Depressive disorder (DD) is a common chronic and highly disabling disease.					
35428308	8	0	theme	16S	1159:1161	arg1	sequencing					1173:1182	16S rRNA gene sequencing	1159:1182	16S rRNA gene sequencing	1159:1182	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	12	1	theme	alkali	1759:1764	arg1	tolerance					1766:1774	wide alkali tolerance	1754:1774	wide alkali tolerance	1754:1774	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	12	2	theme	wide	1649:1652	arg1	thermotolerance					1654:1668	wide thermotolerance	1649:1668	wide thermotolerance	1649:1668	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	8	3	theme	gene	1168:1171	arg1	sequencing					1173:1182	16S rRNA gene sequencing	1159:1182	16S rRNA gene sequencing	1159:1182	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	16	4	theme	gene	2333:2336	arg1	product					2338:2344	the gene product	2329:2344	the gene product	2329:2344	Multiple sequence alignment and homology modeling of the protein sequence revealed that the gene product belonged to the GH11 family, indicating its possible application in bioconversion.					
35428308	18	5	theme	high	2720:2723	arg1	temperatures					2725:2736	high temperatures	2720:2736	high temperatures	2720:2736	The enzyme can be supplemented in industrial applications for biomass conversion at high temperatures and pH (or in processes involving alkali treatment).					
35428308	9	6	theme	xylanase	1200:1207	arg1	activity					1209:1216	The persistent xylanase activity	1185:1216	The persistent xylanase activity	1185:1216	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	2	7	theme	glycosidic	254:263	arg1	bonds					265:269	β-1,4 glycosidic bonds	248:269	β-1,4 glycosidic bonds	248:269	It is primarily composed of xylan linked by β-1,4 glycosidic bonds.					
35428308	7	8	theme	vital	848:852	arg1	enzyme					854:859	this industrially vital enzyme	830:859	this industrially vital enzyme	830:859	In addition, the gene encoding this industrially vital enzyme was isolated and characterized, and its protein structure was analyzed.					
35428308	4	9	dep	act	448:450	arg1	Ramasamy					593:600	Ramasamy	593:600	Ramasamy	593:600	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	16	10	theme	Multiple	2241:2248	arg1	alignment					2259:2267	Multiple sequence alignment	2241:2267	Multiple sequence alignment	2241:2267	Multiple sequence alignment and homology modeling of the protein sequence revealed that the gene product belonged to the GH11 family, indicating its possible application in bioconversion.					
35428308	1	11	theme	polymer	162:168	arg1	one					143:145	one	143:145	one	143:145	BACKGROUND Hemicellulose is one of the copious polymer in lignocellulosic biomass (LCB).					
35428308	1	11	theme	polymer	162:168	arg1	polymer					162:168	the copious polymer	150:168	the copious polymer in lignocellulosic biomass (LCB)	150:201	BACKGROUND Hemicellulose is one of the copious polymer in lignocellulosic biomass (LCB).					
35428308	9	12	from	12 h	1340:1343	arg1	1.0 IU mL-1					1317:1327	1.0 IU mL-1	1317:1327	1.0 IU mL-1	1317:1327	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	9	12	from	12 h	1340:1343	arg1	0.76 IU mL-1					1300:1311	0.76 IU mL-1	1300:1311	0.76 IU mL-1	1300:1311	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	9	12	from	12 h	1340:1343	arg1	activity					1288:1295	the maximum activity	1276:1295	the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively	1276:1386	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	8	13	theme	augmented	998:1006	arg1	sawdust					1008:1014	augmented sawdust	998:1014	augmented sawdust	998:1014	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	17	14	theme	alkali-tolerant	2563:2577	arg1	xylanases					2592:2600	potent and alkali-tolerant thermostable xylanases	2552:2600	potent and alkali-tolerant thermostable xylanases	2552:2600	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	14	15	theme	high	2076:2079	arg1	9.44-9.65					2085:2093	9.44-9.65	2085:2093	9.44-9.65	2085:2093	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	14	15	theme	high	2076:2079	arg1	pI					2081:2082	a high pI	2074:2082	a high pI (9.44-9.65)	2074:2094	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	6	16	theme	thermal	768:774	arg1	springs					776:782	thermal springs	768:782	thermal springs	768:782	RESULTS The xylanase production by two thermophilic bacteria isolated from thermal springs was evaluated.					
35428308	1	17	theme	lignocellulosic	173:187	arg1	LCB					198:200	LCB	198:200	LCB	198:200	BACKGROUND Hemicellulose is one of the copious polymer in lignocellulosic biomass (LCB).					
35428308	1	17	theme	lignocellulosic	173:187	arg1	biomass					189:195	lignocellulosic biomass	173:195	lignocellulosic biomass (LCB)	173:201	BACKGROUND Hemicellulose is one of the copious polymer in lignocellulosic biomass (LCB).					
35428308	9	18	from	6 h	1332:1334	arg1	1.0 IU mL-1					1317:1327	1.0 IU mL-1	1317:1327	1.0 IU mL-1	1317:1327	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	9	18	from	6 h	1332:1334	arg1	0.76 IU mL-1					1300:1311	0.76 IU mL-1	1300:1311	0.76 IU mL-1	1300:1311	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	9	18	from	6 h	1332:1334	arg1	activity					1288:1295	the maximum activity	1276:1295	the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively	1276:1386	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	14	19	theme	23 kDa	2062:2067	arg1	weight					2052:2057	a molecular weight	2040:2057	a molecular weight of 23 kDa	2040:2067	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	10	20	from	pH	1470:1471	arg1	activity					1458:1465	the maximum activity	1446:1465	the maximum activity at pH 6	1446:1473	Both the strains exhibited the maximum activity at pH 6 and a temperature of 50 °C.					
35428308	10	20	from	pH	1470:1471	arg1	temperature					1481:1491	a temperature	1479:1491	a temperature of 50 °C	1479:1500	Both the strains exhibited the maximum activity at pH 6 and a temperature of 50 °C.					
35428308	4	21	theme	robust	455:460	arg1	variants					415:422	Thermostable variants	402:422	Thermostable variants of glycoside hydrolases	402:446	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	4	21	theme	robust	455:460	arg1	catalysts					462:470	robust catalysts	455:470	robust catalysts	455:470	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	4	22	dep	Agric	619:623	arg1	J					625:625	J	625:625	J	625:625	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	17	23	theme	thermostable	2579:2590	arg1	xylanases					2592:2600	potent and alkali-tolerant thermostable xylanases	2552:2600	potent and alkali-tolerant thermostable xylanases	2552:2600	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	12	24	from	%	1685:1685	arg1	80 °C					1714:1718	80 °C	1714:1718	80 °C	1714:1718	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	12	25	dep	80 °C	1714:1718	arg1	to					1711:1712	to	1711:1712	to	1711:1712	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	3	26	from	bonds	325:329	arg1	backbone					344:351	the xylan backbone	334:351	the xylan backbone resulting in complete hydrolysis of the biomass	334:399	Xylanase preferentially cleaves the β-1,4-glycosidic bonds in the xylan backbone resulting in complete hydrolysis of the biomass.					
35428308	0	27	from	bacteria	75:82	arg1	gene					29:32	Glycosyl hydrolase 11 (xynA) gene	0:32	Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria	0:82	Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria isolated from thermal springs.					
35428308	17	28	theme	hot	2511:2513	arg1	springs					2515:2521	hot springs	2511:2521	hot springs of Himachal Pradesh	2511:2541	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	17	29	dep	CONCLUSION	2429:2438	arg1	produced					2543:2550	produced	2543:2550	produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family	2543:2633	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	0	30	theme	xylanase	39:46	arg1	activity					48:55	xylanase activity	39:55	xylanase activity	39:55	Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria isolated from thermal springs.					
35428308	9	31	theme	0.76 IU mL-1	1300:1311	arg1	activity					1288:1295	the maximum activity	1276:1295	the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively	1276:1386	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	14	32	theme	molecular	2042:2050	arg1	weight					2052:2057	a molecular weight	2040:2057	a molecular weight of 23 kDa	2040:2067	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	11	33	theme	KBFB4	1520:1524	arg1	xylanases					1507:1515	The xylanases	1503:1515	The xylanases of KBFB4 and VSDB5	1503:1534	The xylanases of KBFB4 and VSDB5 were thermostable and retained 40% of their activity at 60 °C after incubation for 30 min.					
35428308	11	33	theme	KBFB4	1520:1524	arg1	thermostable					1541:1552	thermostable	1541:1552	thermostable	1541:1552	The xylanases of KBFB4 and VSDB5 were thermostable and retained 40% of their activity at 60 °C after incubation for 30 min.					
35428308	9	34	theme	1.0 IU mL-1	1317:1327	arg1	activity					1288:1295	the maximum activity	1276:1295	the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively	1276:1386	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	8	35	theme	fiber	1027:1031	arg1	biomass					1033:1039	banana fiber biomass	1020:1039	banana fiber biomass	1020:1039	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	5	36	dep	//doi.org/10.29321/MAJ.2020.000382	649:682	arg1	2020					686:689	2020	686:689	2020	686:689	https://doi.org/10.29321/MAJ.2020.000382 , 2020).					
35428308	10	37	theme	maximum	1450:1456	arg1	activity					1458:1465	the maximum activity	1446:1465	the maximum activity at pH 6	1446:1473	Both the strains exhibited the maximum activity at pH 6 and a temperature of 50 °C.					
35428308	12	38	theme	KBFB4	1741:1745	arg1	xylanase					1729:1736	xylanase	1729:1736	xylanase of KBFB4	1729:1745	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	13	39	theme	β-1,4-endoxylanase	1946:1963	arg1	gene					1965:1968	β-1,4-endoxylanase gene	1946:1968	β-1,4-endoxylanase gene	1946:1968	The xylanase (xynA)-encoding gene (650 bp) cloned from both the strains using specific primers showed 98 to 99% homology to β-1,4-endoxylanase gene.					
35428308	13	40	dep	homology	1934:1941	arg1	%					1932:1932	%	1932:1932	%	1932:1932	The xylanase (xynA)-encoding gene (650 bp) cloned from both the strains using specific primers showed 98 to 99% homology to β-1,4-endoxylanase gene.					
35428308	5	41	dep	https	643:647	arg1	//doi.org/10.29321/MAJ.2020.000382					649:682	//doi.org/10.29321/MAJ.2020.000382	649:682	//doi.org/10.29321/MAJ.2020.000382	649:682	https://doi.org/10.29321/MAJ.2020.000382 , 2020).					
35428308	14	42	theme	aliphatic	2159:2167	arg1	56.29					2176:2180	56.29	2176:2180	56.29	2176:2180	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	14	42	theme	aliphatic	2159:2167	arg1	index					2169:2173	greater aliphatic index	2151:2173	greater aliphatic index (56.29)	2151:2181	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	18	43	theme	alkali	2772:2777	arg1	treatment					2779:2787	alkali treatment	2772:2787	alkali treatment	2772:2787	The enzyme can be supplemented in industrial applications for biomass conversion at high temperatures and pH (or in processes involving alkali treatment).					
35428308	13	44	dep	99	1930:1931	arg1	to					1927:1928	to	1927:1928	to	1927:1928	The xylanase (xynA)-encoding gene (650 bp) cloned from both the strains using specific primers showed 98 to 99% homology to β-1,4-endoxylanase gene.					
35428308	0	45	theme	Glycosyl	0:7	arg1	gene					29:32	Glycosyl hydrolase 11 (xynA) gene	0:32	Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria	0:82	Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria isolated from thermal springs.					
35428308	9	46	theme	growth	1348:1353	arg1	12 h					1340:1343	12 h	1340:1343	12 h	1340:1343	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	9	46	theme	growth	1348:1353	arg1	6 h					1332:1334	6 h	1332:1334	6 h	1332:1334	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	4	47	theme	glycoside	427:435	arg1	hydrolases					437:446	glycoside hydrolases	427:446	glycoside hydrolases	427:446	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	4	48	theme	specific	535:542	arg1	chemicals					568:576	specific carbohydrate-containing chemicals	535:576	specific carbohydrate-containing chemicals	535:576	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	13	49	theme	specific	1900:1907	arg1	primers					1909:1915	specific primers	1900:1915	specific primers	1900:1915	The xylanase (xynA)-encoding gene (650 bp) cloned from both the strains using specific primers showed 98 to 99% homology to β-1,4-endoxylanase gene.					
35428308	12	50	from	80 °C	1714:1718	arg1	%					1685:1685	20%	1683:1685	20% of its activity from 60 to 80 °C	1683:1718	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	12	50	from	80 °C	1714:1718	arg1	activity					1694:1701	its activity	1690:1701	its activity from 60 to 80 °C	1690:1718	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	11	51	from	60 °C	1592:1596	arg1	activity					1580:1587	their activity	1574:1587	their activity	1574:1587	The xylanases of KBFB4 and VSDB5 were thermostable and retained 40% of their activity at 60 °C after incubation for 30 min.					
35428308	11	51	from	60 °C	1592:1596	arg1	%					1569:1569	40%	1567:1569	40% of their activity at 60 °C	1567:1596	The xylanases of KBFB4 and VSDB5 were thermostable and retained 40% of their activity at 60 °C after incubation for 30 min.					
35428308	0	52	theme	xynA	23:26	arg1	gene					29:32	Glycosyl hydrolase 11 (xynA) gene	0:32	Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria	0:82	Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria isolated from thermal springs.					
35428308	14	53	theme	Further	1971:1977	arg1	analysis					1989:1996	Further in silico analysis	1971:1996	Further in silico analysis	1971:1996	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	14	54	contain	had	2070:2072	arg2	pI					2081:2082	a high pI	2074:2082	a high pI (9.44-9.65)	2074:2094	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	14	54	contain	had	2070:2072	arg1	protein					2026:2032	the xylanase protein	2013:2032	the xylanase protein	2013:2032	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	14	54	contain	had	2070:2072	arg2	9.44-9.65					2085:2093	9.44-9.65	2085:2093	9.44-9.65	2085:2093	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	17	55	dep	B.	2474:2475	arg1	licheniformis					2477:2489	licheniformis	2477:2489	licheniformis	2477:2489	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	7	56	theme	protein	901:907	arg1	structure					909:917	its protein structure	897:917	its protein structure	897:917	In addition, the gene encoding this industrially vital enzyme was isolated and characterized, and its protein structure was analyzed.					
35428308	12	57	theme	activity	1800:1807	arg1	activity					1800:1807	its activity	1796:1807	its activity	1796:1807	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	12	57	theme	activity	1800:1807	arg1	%					1791:1791	80%	1789:1791	80% of its activity	1789:1807	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	16	58	theme	homology	2273:2280	arg1	modeling					2282:2289	homology modeling	2273:2289	homology modeling	2273:2289	Multiple sequence alignment and homology modeling of the protein sequence revealed that the gene product belonged to the GH11 family, indicating its possible application in bioconversion.					
35428308	12	59	theme	wide	1754:1757	arg1	tolerance					1766:1774	wide alkali tolerance	1754:1774	wide alkali tolerance	1754:1774	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	14	60	theme	alkaline	2117:2124	arg1	nature					2126:2131	the alkaline nature	2113:2131	the alkaline nature of the enzyme	2113:2145	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	0	61	with	gene	29:32	arg1	activity					48:55	xylanase activity	39:55	xylanase activity	39:55	Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria isolated from thermal springs.					
35428308	4	62	theme	Madras	612:617	arg1	Agric					619:623	Madras Agric	612:623	Madras Agric J 107(special):1	612:640	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	16	63	theme	sequence	2306:2313	arg1	modeling					2282:2289	homology modeling	2273:2289	homology modeling	2273:2289	Multiple sequence alignment and homology modeling of the protein sequence revealed that the gene product belonged to the GH11 family, indicating its possible application in bioconversion.					
35428308	16	63	theme	sequence	2306:2313	arg1	alignment					2259:2267	Multiple sequence alignment	2241:2267	Multiple sequence alignment	2241:2267	Multiple sequence alignment and homology modeling of the protein sequence revealed that the gene product belonged to the GH11 family, indicating its possible application in bioconversion.					
35428308	18	64	theme	biomass	2698:2704	arg1	conversion					2706:2715	biomass conversion	2698:2715	biomass conversion	2698:2715	The enzyme can be supplemented in industrial applications for biomass conversion at high temperatures and pH (or in processes involving alkali treatment).					
35428308	9	65	theme	persistent	1189:1198	arg1	activity					1209:1216	The persistent xylanase activity	1185:1216	The persistent xylanase activity	1185:1216	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	8	66	theme	rRNA	1163:1166	arg1	sequencing					1173:1182	16S rRNA gene sequencing	1159:1182	16S rRNA gene sequencing	1159:1182	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	4	67	theme	Thermostable	402:413	arg1	variants					415:422	Thermostable variants	402:422	Thermostable variants of glycoside hydrolases	402:446	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	4	67	theme	Thermostable	402:413	arg1	catalysts					462:470	robust catalysts	455:470	robust catalysts	455:470	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	11	68	theme	VSDB5	1530:1534	arg1	xylanases					1507:1515	The xylanases	1503:1515	The xylanases of KBFB4 and VSDB5	1503:1534	The xylanases of KBFB4 and VSDB5 were thermostable and retained 40% of their activity at 60 °C after incubation for 30 min.					
35428308	11	68	theme	VSDB5	1530:1534	arg1	thermostable					1541:1552	thermostable	1541:1552	thermostable	1541:1552	The xylanases of KBFB4 and VSDB5 were thermostable and retained 40% of their activity at 60 °C after incubation for 30 min.					
35428308	2	69	theme	β-1,4	248:252	arg1	bonds					265:269	β-1,4 glycosidic bonds	248:269	β-1,4 glycosidic bonds	248:269	It is primarily composed of xylan linked by β-1,4 glycosidic bonds.					
35428308	1	70	theme	copious	154:160	arg1	polymer					162:168	the copious polymer	150:168	the copious polymer in lignocellulosic biomass (LCB)	150:201	BACKGROUND Hemicellulose is one of the copious polymer in lignocellulosic biomass (LCB).					
35428308	16	71	theme	GH11	2362:2365	arg1	family					2367:2372	the GH11 family	2358:2372	the GH11 family	2358:2372	Multiple sequence alignment and homology modeling of the protein sequence revealed that the gene product belonged to the GH11 family, indicating its possible application in bioconversion.					
35428308	17	72	theme	GH11	2623:2626	arg1	family					2628:2633	the GH11 family	2619:2633	the GH11 family	2619:2633	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	12	73	contain	had	1645:1647	arg1	Xylanase					1627:1634	Xylanase	1627:1634	Xylanase of VSDB5	1627:1643	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	12	73	contain	had	1645:1647	arg2	thermotolerance					1654:1668	wide thermotolerance	1649:1668	wide thermotolerance	1649:1668	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	18	74	theme	industrial	2670:2679	arg1	applications					2681:2692	industrial applications	2670:2692	industrial applications for biomass conversion	2670:2715	The enzyme can be supplemented in industrial applications for biomass conversion at high temperatures and pH (or in processes involving alkali treatment).					
35428308	8	75	theme	banana	1020:1025	arg1	biomass					1033:1039	banana fiber biomass	1020:1039	banana fiber biomass	1020:1039	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	8	76	dep	VSDB5	1114:1118	arg1	KBFB4					1147:1151	KBFB4	1147:1151	KBFB4	1147:1151	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	16	77	theme	sequence	2250:2257	arg1	alignment					2259:2267	Multiple sequence alignment	2241:2267	Multiple sequence alignment	2241:2267	Multiple sequence alignment and homology modeling of the protein sequence revealed that the gene product belonged to the GH11 family, indicating its possible application in bioconversion.					
35428308	17	78	theme	Pradesh	2535:2541	arg1	springs					2515:2521	hot springs	2511:2521	hot springs of Himachal Pradesh	2511:2541	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	8	79	dep	Bacillus	1096:1103	arg1	subtilis					1105:1112	subtilis	1105:1112	subtilis	1105:1112	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	4	80	dep	Ramasamy	593:600	arg1	al.					605:607	Ramasamy et al.	593:607	Ramasamy et al. in Madras Agric J 107(special):1	593:640	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	6	81	attach	isolated	754:761	arg1	springs					776:782	thermal springs	768:782	thermal springs	768:782	RESULTS The xylanase production by two thermophilic bacteria isolated from thermal springs was evaluated.					
35428308	6	81	attach	isolated	754:761	arg2	bacteria					745:752	two thermophilic bacteria	728:752	two thermophilic bacteria isolated from thermal springs	728:782	RESULTS The xylanase production by two thermophilic bacteria isolated from thermal springs was evaluated.					
35428308	17	82	theme	potent	2552:2557	arg1	xylanases					2592:2600	potent and alkali-tolerant thermostable xylanases	2552:2600	potent and alkali-tolerant thermostable xylanases	2552:2600	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	17	83	theme	B.	2474:2475	arg1	KBFB4					2491:2495	B. licheniformis KBFB4	2474:2495	B. licheniformis KBFB4	2474:2495	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	17	83	theme	B.	2474:2475	arg1	strains					2444:2450	The strains B. subtilis VSDB5 and B. licheniformis KBFB4	2440:2495	The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh	2440:2541	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	14	84	dep	in	1979:1980	arg1	silico					1982:1987	silico	1982:1987	silico	1982:1987	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	13	85	theme	-encoding	1841:1849	arg1	gene					1851:1854	The xylanase (xynA)-encoding gene	1822:1854	The xylanase (xynA)-encoding gene (650 bp) cloned from both the strains using specific primers	1822:1915	The xylanase (xynA)-encoding gene (650 bp) cloned from both the strains using specific primers showed 98 to 99% homology to β-1,4-endoxylanase gene.					
35428308	13	85	theme	-encoding	1841:1849	arg1	650 bp					1857:1862	650 bp	1857:1862	650 bp	1857:1862	The xylanase (xynA)-encoding gene (650 bp) cloned from both the strains using specific primers showed 98 to 99% homology to β-1,4-endoxylanase gene.					
35428308	9	86	theme	maximum	1280:1286	arg1	activity					1288:1295	the maximum activity	1276:1295	the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively	1276:1386	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	1	87	theme	BACKGROUND	115:124	arg1	Hemicellulose					126:138	BACKGROUND Hemicellulose	115:138	BACKGROUND Hemicellulose	115:138	BACKGROUND Hemicellulose is one of the copious polymer in lignocellulosic biomass (LCB).					
35428308	4	88	theme	hydrolases	437:446	arg1	variants					415:422	Thermostable variants	402:422	Thermostable variants of glycoside hydrolases	402:446	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	4	88	theme	hydrolases	437:446	arg1	catalysts					462:470	robust catalysts	455:470	robust catalysts	455:470	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	12	89	theme	activity	1694:1701	arg1	%					1685:1685	20%	1683:1685	20% of its activity from 60 to 80 °C	1683:1718	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	12	89	theme	activity	1694:1701	arg1	activity					1694:1701	its activity	1690:1701	its activity from 60 to 80 °C	1690:1718	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	8	90	attach	isolated	984:991	arg1	biomass					1033:1039	banana fiber biomass	1020:1039	banana fiber biomass	1020:1039	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	8	90	attach	isolated	984:991	arg2	licheniformis					1133:1145	Bacillus licheniformis	1124:1145	Bacillus licheniformis	1124:1145	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	8	90	attach	isolated	984:991	arg2	VSDB5					1114:1118	Bacillus subtilis VSDB5	1096:1118	Bacillus subtilis VSDB5	1096:1118	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	8	90	attach	isolated	984:991	arg1	sawdust					1008:1014	augmented sawdust	998:1014	augmented sawdust	998:1014	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	8	90	attach	isolated	984:991	arg1	springs					1050:1056	hot springs	1046:1056	hot springs of Himachal Pradesh	1046:1076	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	8	90	attach	isolated	984:991	arg2	bacteria					950:957	The thermophilic bacteria	933:957	The thermophilic bacteria producing xylanases	933:977	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	0	91	theme	thermophilic	62:73	arg1	bacteria					75:82	thermophilic bacteria	62:82	thermophilic bacteria	62:82	Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria isolated from thermal springs.					
35428308	1	92	from	polymer	162:168	arg1	LCB					198:200	LCB	198:200	LCB	198:200	BACKGROUND Hemicellulose is one of the copious polymer in lignocellulosic biomass (LCB).					
35428308	1	92	from	polymer	162:168	arg1	biomass					189:195	lignocellulosic biomass	173:195	lignocellulosic biomass (LCB)	173:201	BACKGROUND Hemicellulose is one of the copious polymer in lignocellulosic biomass (LCB).					
35428308	3	93	theme	β-1,4-glycosidic	308:323	arg1	bonds					325:329	the β-1,4-glycosidic bonds	304:329	the β-1,4-glycosidic bonds in the xylan backbone resulting in complete hydrolysis of the biomass	304:399	Xylanase preferentially cleaves the β-1,4-glycosidic bonds in the xylan backbone resulting in complete hydrolysis of the biomass.					
35428308	17	94	dep	B.	2452:2453	arg1	subtilis					2455:2462	subtilis	2455:2462	subtilis	2455:2462	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	8	95	theme	hot	1046:1048	arg1	springs					1050:1056	hot springs	1046:1056	hot springs of Himachal Pradesh	1046:1076	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	8	96	theme	thermophilic	937:948	arg1	VSDB5					1114:1118	Bacillus subtilis VSDB5	1096:1118	Bacillus subtilis VSDB5	1096:1118	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	8	96	theme	thermophilic	937:948	arg1	licheniformis					1133:1145	Bacillus licheniformis	1124:1145	Bacillus licheniformis	1124:1145	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	8	96	theme	thermophilic	937:948	arg1	bacteria					950:957	The thermophilic bacteria	933:957	The thermophilic bacteria producing xylanases	933:977	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	0	97	theme	thermal	98:104	arg1	springs					106:112	thermal springs	98:112	thermal springs	98:112	Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria isolated from thermal springs.					
35428308	12	98	theme	VSDB5	1639:1643	arg1	Xylanase					1627:1634	Xylanase	1627:1634	Xylanase of VSDB5	1627:1643	Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10.					
35428308	3	99	theme	xylan	338:342	arg1	backbone					344:351	the xylan backbone	334:351	the xylan backbone resulting in complete hydrolysis of the biomass	334:399	Xylanase preferentially cleaves the β-1,4-glycosidic bonds in the xylan backbone resulting in complete hydrolysis of the biomass.					
35428308	14	100	theme	xylanase	2017:2024	arg1	protein					2026:2032	the xylanase protein	2013:2032	the xylanase protein	2013:2032	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	17	101	theme	B.	2452:2453	arg1	strains					2444:2450	The strains B. subtilis VSDB5 and B. licheniformis KBFB4	2440:2495	The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh	2440:2541	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	17	101	theme	B.	2452:2453	arg1	VSDB5					2464:2468	B. subtilis VSDB5	2452:2468	B. subtilis VSDB5	2452:2468	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	14	102	theme	greater	2151:2157	arg1	56.29					2176:2180	56.29	2176:2180	56.29	2176:2180	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	14	102	theme	greater	2151:2157	arg1	index					2169:2173	greater aliphatic index	2151:2173	greater aliphatic index (56.29)	2151:2181	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	16	103	from	application	2399:2409	arg1	bioconversion					2414:2426	bioconversion	2414:2426	bioconversion	2414:2426	Multiple sequence alignment and homology modeling of the protein sequence revealed that the gene product belonged to the GH11 family, indicating its possible application in bioconversion.					
35428308	0	104	theme	hydrolase	9:17	arg1	gene					29:32	Glycosyl hydrolase 11 (xynA) gene	0:32	Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria	0:82	Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria isolated from thermal springs.					
35428308	8	105	theme	Pradesh	1070:1076	arg1	springs					1050:1056	hot springs	1046:1056	hot springs of Himachal Pradesh	1046:1076	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	13	106	dep	%	1932:1932	arg1	99					1930:1931	99	1930:1931	99	1930:1931	The xylanase (xynA)-encoding gene (650 bp) cloned from both the strains using specific primers showed 98 to 99% homology to β-1,4-endoxylanase gene.					
35428308	4	107	theme	carbohydrate-containing	544:566	arg1	chemicals					568:576	specific carbohydrate-containing chemicals	535:576	specific carbohydrate-containing chemicals	535:576	Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1.					
35428308	6	108	dep	RESULTS	693:699	arg1	evaluated					788:796	evaluated	788:796	was evaluated	784:796	RESULTS The xylanase production by two thermophilic bacteria isolated from thermal springs was evaluated.					
35428308	14	109	theme	enzyme	2140:2145	arg1	nature					2126:2131	the alkaline nature	2113:2131	the alkaline nature of the enzyme	2113:2145	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	14	109	theme	enzyme	2140:2145	arg1	56.29					2176:2180	56.29	2176:2180	56.29	2176:2180	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	14	109	theme	enzyme	2140:2145	arg1	index					2169:2173	greater aliphatic index	2151:2173	greater aliphatic index (56.29)	2151:2181	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	17	110	dep	strains	2444:2450	arg1	KBFB4					2491:2495	B. licheniformis KBFB4	2474:2495	B. licheniformis KBFB4	2474:2495	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	17	110	dep	strains	2444:2450	arg1	strains					2444:2450	The strains B. subtilis VSDB5 and B. licheniformis KBFB4	2440:2495	The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh	2440:2541	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	17	110	dep	strains	2444:2450	arg1	VSDB5					2464:2468	B. subtilis VSDB5	2452:2468	B. subtilis VSDB5	2452:2468	CONCLUSION The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family.					
35428308	3	111	theme	complete	366:373	arg1	hydrolysis					375:384	complete hydrolysis	366:384	complete hydrolysis of the biomass	366:399	Xylanase preferentially cleaves the β-1,4-glycosidic bonds in the xylan backbone resulting in complete hydrolysis of the biomass.					
35428308	10	112	theme	50 °C	1496:1500	arg1	activity					1458:1465	the maximum activity	1446:1465	the maximum activity at pH 6	1446:1473	Both the strains exhibited the maximum activity at pH 6 and a temperature of 50 °C.					
35428308	10	112	theme	50 °C	1496:1500	arg1	temperature					1481:1491	a temperature	1479:1491	a temperature of 50 °C	1479:1500	Both the strains exhibited the maximum activity at pH 6 and a temperature of 50 °C.					
35428308	8	113	theme	Bacillus	1096:1103	arg1	VSDB5					1114:1118	Bacillus subtilis VSDB5	1096:1118	Bacillus subtilis VSDB5	1096:1118	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	8	113	theme	Bacillus	1096:1103	arg1	bacteria					950:957	The thermophilic bacteria	933:957	The thermophilic bacteria producing xylanases	933:977	The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing.					
35428308	14	114	dep	had	2070:2072	arg1	explained					2103:2111	explained	2103:2111	explained the alkaline nature of the enzyme and greater aliphatic index (56.29)	2103:2181	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	14	115	theme	in	1979:1980	arg1	analysis					1989:1996	Further in silico analysis	1971:1996	Further in silico analysis	1971:1996	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	11	116	theme	activity	1580:1587	arg1	activity					1580:1587	their activity	1574:1587	their activity	1574:1587	The xylanases of KBFB4 and VSDB5 were thermostable and retained 40% of their activity at 60 °C after incubation for 30 min.					
35428308	11	116	theme	activity	1580:1587	arg1	%					1569:1569	40%	1567:1569	40% of their activity at 60 °C	1567:1596	The xylanases of KBFB4 and VSDB5 were thermostable and retained 40% of their activity at 60 °C after incubation for 30 min.					
35428308	3	117	theme	biomass	393:399	arg1	hydrolysis					375:384	complete hydrolysis	366:384	complete hydrolysis of the biomass	366:399	Xylanase preferentially cleaves the β-1,4-glycosidic bonds in the xylan backbone resulting in complete hydrolysis of the biomass.					
35428308	6	118	theme	thermophilic	732:743	arg1	bacteria					745:752	two thermophilic bacteria	728:752	two thermophilic bacteria isolated from thermal springs	728:782	RESULTS The xylanase production by two thermophilic bacteria isolated from thermal springs was evaluated.					
35428308	16	119	theme	possible	2390:2397	arg1	application					2399:2409	its possible application	2386:2409	its possible application in bioconversion	2386:2426	Multiple sequence alignment and homology modeling of the protein sequence revealed that the gene product belonged to the GH11 family, indicating its possible application in bioconversion.					
35428308	14	120	with	protein	2026:2032	arg1	weight					2052:2057	a molecular weight	2040:2057	a molecular weight of 23 kDa	2040:2067	Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44-9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29).					
35428308	16	121	theme	protein	2298:2304	arg1	sequence					2306:2313	the protein sequence	2294:2313	the protein sequence	2294:2313	Multiple sequence alignment and homology modeling of the protein sequence revealed that the gene product belonged to the GH11 family, indicating its possible application in bioconversion.					
35428308	9	122	theme	submerged	1395:1403	arg1	fermentation					1405:1416	submerged fermentation	1395:1416	submerged fermentation	1395:1416	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	9	123	from	activity	1288:1295	arg1	12 h					1340:1343	12 h	1340:1343	12 h	1340:1343	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	9	123	from	activity	1288:1295	arg1	6 h					1332:1334	6 h	1332:1334	6 h	1332:1334	The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL-1 and 1.0 IU mL-1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation.					
35428308	6	124	theme	xylanase	705:712	arg1	production					714:723	The xylanase production	701:723	The xylanase production by two thermophilic bacteria isolated from thermal springs	701:782	RESULTS The xylanase production by two thermophilic bacteria isolated from thermal springs was evaluated.					
36427150	1	0	theme	used	161:164	arg1	tool					166:169	a widely used tool	152:169	a widely used tool for studying nucleosome structure, dynamics, and interactions	152:231	Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions.					
36427150	1	0	theme	used	161:164	arg1	Reconstitution					85:98	Reconstitution	85:98	Reconstitution of nucleosomes from recombinant histones and DNA	85:147	Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions.					
36427150	2	1	with	nucleosomes	299:309	arg1	compositions					324:335	defined compositions	316:335	defined compositions	316:335	Preparation of reconstituted nucleosomes allows for the study of nucleosomes with defined compositions.					
36427150	3	2	theme	Widom	459:463	arg1	DNA					469:471	147 bp Widom 601 DNA	452:471	147 bp Widom 601 DNA	452:471	Here, we describe methods for refolding recombinant human histones, reconstituting nucleosome core particles with 147 bp Widom 601 DNA, and purification via sucrose gradient.					
36427150	0	3	theme	Recombinant	45:55	arg1	Histones					57:64	Recombinant Histones	45:64	Recombinant Histones	45:64	Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA.					
36427150	3	4	with	particles	437:445	arg1	DNA					469:471	147 bp Widom 601 DNA	452:471	147 bp Widom 601 DNA	452:471	Here, we describe methods for refolding recombinant human histones, reconstituting nucleosome core particles with 147 bp Widom 601 DNA, and purification via sucrose gradient.					
36427150	3	5	theme	601	465:467	arg1	DNA					469:471	147 bp Widom 601 DNA	452:471	147 bp Widom 601 DNA	452:471	Here, we describe methods for refolding recombinant human histones, reconstituting nucleosome core particles with 147 bp Widom 601 DNA, and purification via sucrose gradient.					
36427150	0	6	theme	Core	11:14	arg1	Particle					16:23	Nucleosome Core Particle	0:23	Nucleosome Core Particle	0:23	Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA.					
36427150	1	7	from	DNA	145:147	arg1	tool					166:169	a widely used tool	152:169	a widely used tool for studying nucleosome structure, dynamics, and interactions	152:231	Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions.					
36427150	1	7	from	DNA	145:147	arg1	Reconstitution					85:98	Reconstitution	85:98	Reconstitution of nucleosomes from recombinant histones and DNA	85:147	Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions.					
36427150	0	8	theme	Nucleosome	0:9	arg1	Particle					16:23	Nucleosome Core Particle	0:23	Nucleosome Core Particle	0:23	Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA.					
36427150	2	9	theme	defined	316:322	arg1	compositions					324:335	defined compositions	316:335	defined compositions	316:335	Preparation of reconstituted nucleosomes allows for the study of nucleosomes with defined compositions.					
36427150	1	10	theme	nucleosome	184:193	arg1	structure					195:203	nucleosome structure	184:203	nucleosome structure	184:203	Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions.					
36427150	3	11	theme	recombinant	378:388	arg1	histones					396:403	recombinant human histones	378:403	recombinant human histones	378:403	Here, we describe methods for refolding recombinant human histones, reconstituting nucleosome core particles with 147 bp Widom 601 DNA, and purification via sucrose gradient.					
36427150	1	12	from	histones	132:139	arg1	tool					166:169	a widely used tool	152:169	a widely used tool for studying nucleosome structure, dynamics, and interactions	152:231	Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions.					
36427150	1	12	from	histones	132:139	arg1	Reconstitution					85:98	Reconstitution	85:98	Reconstitution of nucleosomes from recombinant histones and DNA	85:147	Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions.					
36427150	1	13	theme	nucleosomes	103:113	arg1	tool					166:169	a widely used tool	152:169	a widely used tool for studying nucleosome structure, dynamics, and interactions	152:231	Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions.					
36427150	1	13	theme	nucleosomes	103:113	arg1	Reconstitution					85:98	Reconstitution	85:98	Reconstitution of nucleosomes from recombinant histones and DNA	85:147	Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions.					
36427150	3	14	theme	human	390:394	arg1	histones					396:403	recombinant human histones	378:403	recombinant human histones	378:403	Here, we describe methods for refolding recombinant human histones, reconstituting nucleosome core particles with 147 bp Widom 601 DNA, and purification via sucrose gradient.					
36427150	2	15	theme	nucleosomes	299:309	arg1	study					290:294	the study	286:294	the study of nucleosomes with defined compositions	286:335	Preparation of reconstituted nucleosomes allows for the study of nucleosomes with defined compositions.					
36427150	3	16	theme	core	432:435	arg1	particles					437:445	nucleosome core particles	421:445	nucleosome core particles with 147 bp Widom 601 DNA	421:471	Here, we describe methods for refolding recombinant human histones, reconstituting nucleosome core particles with 147 bp Widom 601 DNA, and purification via sucrose gradient.					
36427150	3	17	theme	147 bp	452:457	arg1	DNA					469:471	147 bp Widom 601 DNA	452:471	147 bp Widom 601 DNA	452:471	Here, we describe methods for refolding recombinant human histones, reconstituting nucleosome core particles with 147 bp Widom 601 DNA, and purification via sucrose gradient.					
36427150	0	18	with	Reconstitution	25:38	arg1	DNA					80:82	Widom 601 DNA	70:82	Widom 601 DNA	70:82	Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA.					
36427150	0	18	with	Reconstitution	25:38	arg1	Histones					57:64	Recombinant Histones	45:64	Recombinant Histones	45:64	Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA.					
36427150	1	19	theme	recombinant	120:130	arg1	histones					132:139	recombinant histones	120:139	recombinant histones	120:139	Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions.					
36427150	3	20	theme	nucleosome	421:430	arg1	particles					437:445	nucleosome core particles	421:445	nucleosome core particles with 147 bp Widom 601 DNA	421:471	Here, we describe methods for refolding recombinant human histones, reconstituting nucleosome core particles with 147 bp Widom 601 DNA, and purification via sucrose gradient.					
36427150	2	21	theme	nucleosomes	263:273	arg1	Preparation					234:244	Preparation	234:244	Preparation of reconstituted nucleosomes	234:273	Preparation of reconstituted nucleosomes allows for the study of nucleosomes with defined compositions.					
36427150	3	22	theme	sucrose	495:501	arg1	gradient					503:510	sucrose gradient	495:510	sucrose gradient	495:510	Here, we describe methods for refolding recombinant human histones, reconstituting nucleosome core particles with 147 bp Widom 601 DNA, and purification via sucrose gradient.					
36427150	2	23	theme	reconstituted	249:261	arg1	nucleosomes					263:273	reconstituted nucleosomes	249:273	reconstituted nucleosomes	249:273	Preparation of reconstituted nucleosomes allows for the study of nucleosomes with defined compositions.					
36427150	0	24	theme	Widom	70:74	arg1	DNA					80:82	Widom 601 DNA	70:82	Widom 601 DNA	70:82	Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA.					
36801305	4	0	theme	esterification	573:586	arg1	process					588:594	esterification process	573:594	esterification process	573:594	High-pressure homogenization process was applied to reduce bacterial nanocellulose size and esterification process was carried out to produce cellulose acetate.					
36801305	7	1	theme	16°	1105:1107	arg1	angles					1087:1092	diffraction angles	1075:1092	diffraction angles of 14° and 16°	1075:1107	The results showed that the main cellulose structure was identified at the diffraction angle 22° and the cellulose structure slightly changed at the peak of diffraction angles of 14° and 16°.					
36801305	1	2	theme	cellulose	173:181	arg1	production					183:192	bacterial cellulose production	163:192	bacterial cellulose production	163:192	Agricultural waste is considered a promising source for bacterial cellulose production.					
36801305	8	3	from	change	1275:1280	arg1	membrane					1309:1316	membrane	1309:1316	membrane	1309:1316	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	8	3	from	change	1275:1280	arg1	group					1300:1304	the functional group	1285:1304	the functional group of membrane	1285:1316	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	0	4	theme	graphene	83:90	arg1	reinforcement					92:104	graphene reinforcement	83:104	graphene reinforcement	83:104	Properties of bacterial cellulose acetate nanocomposite with TiO2 nanoparticle and graphene reinforcement.					
36801305	9	5	theme	surface	1334:1340	arg1	morphology					1342:1351	the surface morphology	1330:1351	the surface morphology of membrane	1330:1363	Similarly, the surface morphology of membrane became rougher with the structure of mesoporous membrane.					
36801305	10	6	theme	membrane	1538:1545	arg1	effectiveness					1507:1519	bacterial filtration effectiveness	1486:1519	bacterial filtration effectiveness	1486:1519	Moreover, adding TiO2 and graphene increases crystallinity and bacterial filtration effectiveness of nanocomposite membrane.					
36801305	10	6	theme	membrane	1538:1545	arg1	crystallinity					1468:1480	crystallinity	1468:1480	crystallinity	1468:1480	Moreover, adding TiO2 and graphene increases crystallinity and bacterial filtration effectiveness of nanocomposite membrane.					
36801305	6	7	theme	plate	898:902	arg1	method					910:915	the plate count method	894:915	the plate count method	894:915	The nanocomposite membrane was characterized using an FTIR, SEM, XRD, BET, tensile testing, and bacterial filtration effectiveness using the plate count method.					
36801305	10	8	theme	bacterial	1486:1494	arg1	effectiveness					1507:1519	bacterial filtration effectiveness	1486:1519	bacterial filtration effectiveness	1486:1519	Moreover, adding TiO2 and graphene increases crystallinity and bacterial filtration effectiveness of nanocomposite membrane.					
36801305	7	9	theme	main	946:949	arg1	structure					961:969	the main cellulose structure	942:969	the main cellulose structure	942:969	The results showed that the main cellulose structure was identified at the diffraction angle 22° and the cellulose structure slightly changed at the peak of diffraction angles of 14° and 16°.					
36801305	8	10	theme	functional	1205:1214	arg1	analysis					1222:1229	the functional group analysis	1201:1229	the functional group analysis	1201:1229	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	6	11	theme	count	904:908	arg1	method					910:915	the plate count method	894:915	the plate count method	894:915	The nanocomposite membrane was characterized using an FTIR, SEM, XRD, BET, tensile testing, and bacterial filtration effectiveness using the plate count method.					
36801305	10	12	theme	filtration	1496:1505	arg1	effectiveness					1507:1519	bacterial filtration effectiveness	1486:1519	bacterial filtration effectiveness	1486:1519	Moreover, adding TiO2 and graphene increases crystallinity and bacterial filtration effectiveness of nanocomposite membrane.					
36801305	2	13	from	filtration	368:377	arg1	waters					382:387	waters	382:387	waters	382:387	This study aims to observe the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for bacterial filtration in waters.					
36801305	2	14	theme	acetate-based	316:328	arg1	membranes					344:352	bacterial cellulose acetate-based nanocomposite membranes	296:352	bacterial cellulose acetate-based nanocomposite membranes	296:352	This study aims to observe the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for bacterial filtration in waters.					
36801305	7	15	theme	cellulose	951:959	arg1	structure					961:969	the main cellulose structure	942:969	the main cellulose structure	942:969	The results showed that the main cellulose structure was identified at the diffraction angle 22° and the cellulose structure slightly changed at the peak of diffraction angles of 14° and 16°.					
36801305	4	16	theme	nanocellulose	550:562	arg1	size					564:567	bacterial nanocellulose size	540:567	bacterial nanocellulose size	540:567	High-pressure homogenization process was applied to reduce bacterial nanocellulose size and esterification process was carried out to produce cellulose acetate.					
36801305	7	17	theme	14°	1097:1099	arg1	angles					1087:1092	diffraction angles	1075:1092	diffraction angles of 14° and 16°	1075:1107	The results showed that the main cellulose structure was identified at the diffraction angle 22° and the cellulose structure slightly changed at the peak of diffraction angles of 14° and 16°.					
36801305	7	18	theme	cellulose	1023:1031	arg1	structure					1033:1041	the cellulose structure	1019:1041	the cellulose structure	1019:1041	The results showed that the main cellulose structure was identified at the diffraction angle 22° and the cellulose structure slightly changed at the peak of diffraction angles of 14° and 16°.					
36801305	2	19	theme	cellulose	306:314	arg1	membranes					344:352	bacterial cellulose acetate-based nanocomposite membranes	296:352	bacterial cellulose acetate-based nanocomposite membranes	296:352	This study aims to observe the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for bacterial filtration in waters.					
36801305	2	20	from	influence	226:234	arg1	characteristic					278:291	the characteristic	274:291	the characteristic of bacterial cellulose acetate-based nanocomposite membranes	274:352	This study aims to observe the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for bacterial filtration in waters.					
36801305	4	21	theme	bacterial	540:548	arg1	size					564:567	bacterial nanocellulose size	540:567	bacterial nanocellulose size	540:567	High-pressure homogenization process was applied to reduce bacterial nanocellulose size and esterification process was carried out to produce cellulose acetate.					
36801305	2	22	theme	bacterial	296:304	arg1	membranes					344:352	bacterial cellulose acetate-based nanocomposite membranes	296:352	bacterial cellulose acetate-based nanocomposite membranes	296:352	This study aims to observe the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for bacterial filtration in waters.					
36801305	8	23	theme	several	1243:1249	arg1	shifts					1256:1261	several peak shifts	1243:1261	several peak shifts	1243:1261	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	8	24	theme	group	1216:1220	arg1	analysis					1222:1229	the functional group analysis	1201:1229	the functional group analysis	1201:1229	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	6	25	theme	filtration	863:872	arg1	effectiveness					874:886	bacterial filtration effectiveness	853:886	bacterial filtration effectiveness	853:886	The nanocomposite membrane was characterized using an FTIR, SEM, XRD, BET, tensile testing, and bacterial filtration effectiveness using the plate count method.					
36801305	8	26	dep	%	1184:1184	arg1	to					1186:1187	to	1186:1187	to	1186:1187	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	3	27	theme	Bacterial	390:398	arg1	cellulose					400:408	Bacterial cellulose	390:408	Bacterial cellulose	390:408	Bacterial cellulose was produced from the pineapple peel waste using fermentation process.					
36801305	3	28	theme	pineapple	432:440	arg1	process					472:478	the pineapple peel waste using fermentation process	428:478	the pineapple peel waste using fermentation process	428:478	Bacterial cellulose was produced from the pineapple peel waste using fermentation process.					
36801305	5	29	theme	graphene	732:739	arg1	%					754:754	graphene nanopowder 1 %	732:754	graphene nanopowder 1 %	732:754	Nanocomposite membranes were synthesized with reinforcement of TiO2 nanoparticles 1 % and graphene nanopowder 1 %.					
36801305	6	30	theme	bacterial	853:861	arg1	effectiveness					874:886	bacterial filtration effectiveness	853:886	bacterial filtration effectiveness	853:886	The nanocomposite membrane was characterized using an FTIR, SEM, XRD, BET, tensile testing, and bacterial filtration effectiveness using the plate count method.					
36801305	2	31	theme	bacterial	358:366	arg1	filtration					368:377	bacterial filtration	358:377	bacterial filtration in waters	358:387	This study aims to observe the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for bacterial filtration in waters.					
36801305	9	32	theme	membrane	1356:1363	arg1	morphology					1342:1351	the surface morphology	1330:1351	the surface morphology of membrane	1330:1363	Similarly, the surface morphology of membrane became rougher with the structure of mesoporous membrane.					
36801305	3	33	theme	peel	442:445	arg1	process					472:478	the pineapple peel waste using fermentation process	428:478	the pineapple peel waste using fermentation process	428:478	Bacterial cellulose was produced from the pineapple peel waste using fermentation process.					
36801305	5	34	theme	nanopowder	741:750	arg1	%					754:754	graphene nanopowder 1 %	732:754	graphene nanopowder 1 %	732:754	Nanocomposite membranes were synthesized with reinforcement of TiO2 nanoparticles 1 % and graphene nanopowder 1 %.					
36801305	7	35	theme	angle	1005:1009	arg1	22°					1011:1013	the diffraction angle 22°	989:1013	the diffraction angle 22°	989:1013	The results showed that the main cellulose structure was identified at the diffraction angle 22° and the cellulose structure slightly changed at the peak of diffraction angles of 14° and 16°.					
36801305	1	36	theme	Agricultural	107:118	arg1	waste					120:124	Agricultural waste	107:124	Agricultural waste	107:124	Agricultural waste is considered a promising source for bacterial cellulose production.					
36801305	8	37	theme	functional	1289:1298	arg1	membrane					1309:1316	membrane	1309:1316	membrane	1309:1316	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	8	37	theme	functional	1289:1298	arg1	group					1300:1304	the functional group	1285:1304	the functional group of membrane	1285:1316	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	0	38	theme	cellulose	24:32	arg1	acetate					34:40	bacterial cellulose acetate	14:40	bacterial cellulose acetate	14:40	Properties of bacterial cellulose acetate nanocomposite with TiO2 nanoparticle and graphene reinforcement.					
36801305	8	39	theme	bacterial	1144:1152	arg1	cellulose					1154:1162	bacterial cellulose	1144:1162	bacterial cellulose	1144:1162	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	8	40	theme	peak	1251:1254	arg1	shifts					1256:1261	several peak shifts	1243:1261	several peak shifts	1243:1261	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	2	41	theme	membranes	344:352	arg1	characteristic					278:291	the characteristic	274:291	the characteristic of bacterial cellulose acetate-based nanocomposite membranes	274:352	This study aims to observe the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for bacterial filtration in waters.					
36801305	10	42	theme	nanocomposite	1524:1536	arg1	membrane					1538:1545	nanocomposite membrane	1524:1545	nanocomposite membrane	1524:1545	Moreover, adding TiO2 and graphene increases crystallinity and bacterial filtration effectiveness of nanocomposite membrane.					
36801305	8	43	theme	membrane	1309:1316	arg1	membrane					1309:1316	membrane	1309:1316	membrane	1309:1316	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	8	43	theme	membrane	1309:1316	arg1	group					1300:1304	the functional group	1285:1304	the functional group of membrane	1285:1316	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	0	44	theme	bacterial	14:22	arg1	acetate					34:40	bacterial cellulose acetate	14:40	bacterial cellulose acetate	14:40	Properties of bacterial cellulose acetate nanocomposite with TiO2 nanoparticle and graphene reinforcement.					
36801305	7	45	theme	diffraction	1075:1085	arg1	angles					1087:1092	diffraction angles	1075:1092	diffraction angles of 14° and 16°	1075:1107	The results showed that the main cellulose structure was identified at the diffraction angle 22° and the cellulose structure slightly changed at the peak of diffraction angles of 14° and 16°.					
36801305	2	46	theme	nanocomposite	330:342	arg1	membranes					344:352	bacterial cellulose acetate-based nanocomposite membranes	296:352	bacterial cellulose acetate-based nanocomposite membranes	296:352	This study aims to observe the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for bacterial filtration in waters.					
36801305	3	47	theme	fermentation	459:470	arg1	process					472:478	the pineapple peel waste using fermentation process	428:478	the pineapple peel waste using fermentation process	428:478	Bacterial cellulose was produced from the pineapple peel waste using fermentation process.					
36801305	6	48	theme	nanocomposite	761:773	arg1	membrane					775:782	The nanocomposite membrane	757:782	The nanocomposite membrane	757:782	The nanocomposite membrane was characterized using an FTIR, SEM, XRD, BET, tensile testing, and bacterial filtration effectiveness using the plate count method.					
36801305	0	49	theme	acetate	34:40	arg1	Properties					0:9	Properties	0:9	Properties of bacterial cellulose acetate	0:40	Properties of bacterial cellulose acetate nanocomposite with TiO2 nanoparticle and graphene reinforcement.					
36801305	9	50	theme	mesoporous	1402:1411	arg1	membrane					1413:1420	mesoporous membrane	1402:1420	mesoporous membrane	1402:1420	Similarly, the surface morphology of membrane became rougher with the structure of mesoporous membrane.					
36801305	8	51	theme	cellulose	1154:1162	arg1	crystallinity					1127:1139	the crystallinity	1123:1139	the crystallinity of bacterial cellulose	1123:1162	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	2	52	theme	nanoparticles	244:256	arg1	influence					226:234	the influence	222:234	the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes	222:352	This study aims to observe the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for bacterial filtration in waters.					
36801305	0	53	theme	TiO2	61:64	arg1	nanoparticle					66:77	TiO2 nanoparticle	61:77	TiO2 nanoparticle	61:77	Properties of bacterial cellulose acetate nanocomposite with TiO2 nanoparticle and graphene reinforcement.					
36801305	9	54	theme	membrane	1413:1420	arg1	structure					1389:1397	the structure	1385:1397	the structure of mesoporous membrane	1385:1420	Similarly, the surface morphology of membrane became rougher with the structure of mesoporous membrane.					
36801305	8	55	theme	72.5 	1179:1183	arg1	%					1184:1184	%	1184:1184	%	1184:1184	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	8	56	theme	75.9 	1189:1193	arg1	%					1184:1184	%	1184:1184	%	1184:1184	In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane.					
36801305	2	57	theme	TiO2	239:242	arg1	nanoparticles					244:256	TiO2 nanoparticles	239:256	TiO2 nanoparticles	239:256	This study aims to observe the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for bacterial filtration in waters.					
36801305	6	58	theme	tensile	832:838	arg1	testing					840:846	tensile testing	832:846	tensile testing	832:846	The nanocomposite membrane was characterized using an FTIR, SEM, XRD, BET, tensile testing, and bacterial filtration effectiveness using the plate count method.					
36801305	3	59	theme	using	453:457	arg1	process					472:478	the pineapple peel waste using fermentation process	428:478	the pineapple peel waste using fermentation process	428:478	Bacterial cellulose was produced from the pineapple peel waste using fermentation process.					
36801305	4	60	theme	cellulose	623:631	arg1	acetate					633:639	cellulose acetate	623:639	cellulose acetate	623:639	High-pressure homogenization process was applied to reduce bacterial nanocellulose size and esterification process was carried out to produce cellulose acetate.					
36801305	4	61	theme	High-pressure	481:493	arg1	process					510:516	High-pressure homogenization process	481:516	High-pressure homogenization process	481:516	High-pressure homogenization process was applied to reduce bacterial nanocellulose size and esterification process was carried out to produce cellulose acetate.					
36801305	5	62	theme	Nanocomposite	642:654	arg1	membranes					656:664	Nanocomposite membranes	642:664	Nanocomposite membranes	642:664	Nanocomposite membranes were synthesized with reinforcement of TiO2 nanoparticles 1 % and graphene nanopowder 1 %.					
36801305	5	63	theme	TiO2	705:708	arg1	reinforcement					688:700	reinforcement	688:700	reinforcement of TiO2	688:708	Nanocomposite membranes were synthesized with reinforcement of TiO2 nanoparticles 1 % and graphene nanopowder 1 %.					
36801305	1	64	theme	promising	142:150	arg1	source					152:157	a promising source	140:157	a promising source for bacterial cellulose production	140:192	Agricultural waste is considered a promising source for bacterial cellulose production.					
36801305	7	65	theme	diffraction	993:1003	arg1	22°					1011:1013	the diffraction angle 22°	989:1013	the diffraction angle 22°	989:1013	The results showed that the main cellulose structure was identified at the diffraction angle 22° and the cellulose structure slightly changed at the peak of diffraction angles of 14° and 16°.					
36801305	7	66	theme	angles	1087:1092	arg1	peak					1067:1070	the peak	1063:1070	the peak of diffraction angles of 14° and 16°	1063:1107	The results showed that the main cellulose structure was identified at the diffraction angle 22° and the cellulose structure slightly changed at the peak of diffraction angles of 14° and 16°.					
36801305	4	67	theme	homogenization	495:508	arg1	process					510:516	High-pressure homogenization process	481:516	High-pressure homogenization process	481:516	High-pressure homogenization process was applied to reduce bacterial nanocellulose size and esterification process was carried out to produce cellulose acetate.					
36801305	9	68	with	rougher	1372:1378	arg1	structure					1389:1397	the structure	1385:1397	the structure of mesoporous membrane	1385:1420	Similarly, the surface morphology of membrane became rougher with the structure of mesoporous membrane.					
36801305	3	69	theme	waste	447:451	arg1	process					472:478	the pineapple peel waste using fermentation process	428:478	the pineapple peel waste using fermentation process	428:478	Bacterial cellulose was produced from the pineapple peel waste using fermentation process.					
36801305	2	70	theme	graphene	262:269	arg1	influence					226:234	the influence	222:234	the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes	222:352	This study aims to observe the influence of TiO2 nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for bacterial filtration in waters.					
36801305	1	71	theme	bacterial	163:171	arg1	production					183:192	bacterial cellulose production	163:192	bacterial cellulose production	163:192	Agricultural waste is considered a promising source for bacterial cellulose production.					
36265178	7	0	theme	healing	1375:1381	arg1	stages					1359:1364	the last stages	1350:1364	the last stages of wound healing	1350:1381	Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing.					
36265178	4	1	theme	fast	792:795	arg1	absorption					797:806	fast absorption	792:806	fast absorption of blood and exudate	792:827	SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate.					
36265178	3	2	theme	rapid	570:574	arg1	coagulation					582:592	rapid blood coagulation	570:592	rapid blood coagulation	570:592	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	4	3	theme	ultrathin	659:667	arg1	shells					669:674	their ultrathin shells	653:674	their ultrathin shells	653:674	SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate.					
36265178	5	4	theme	excellent	862:870	arg1	properties					883:892	excellent hemostatic properties	862:892	excellent hemostatic properties for acute hemorrhage	862:913	These features endow SAAMs with excellent hemostatic properties for acute hemorrhage.					
36265178	5	5	theme	hemostatic	872:881	arg1	properties					883:892	excellent hemostatic properties	862:892	excellent hemostatic properties for acute hemorrhage	862:913	These features endow SAAMs with excellent hemostatic properties for acute hemorrhage.					
36265178	3	6	theme	blood	576:580	arg1	coagulation					582:592	rapid blood coagulation	570:592	rapid blood coagulation	570:592	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	8	7	theme	wound	1641:1645	arg1	treatment					1647:1655	wound treatment	1641:1655	wound treatment	1641:1655	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	3	8	theme	multiphase	395:404	arg1	strategy					431:438	a novel strategy	423:438	a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing	423:610	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	8	theme	multiphase	395:404	arg1	microfluidics					406:418	all-aqueous multiphase microfluidics	383:418	all-aqueous multiphase microfluidics	383:418	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	8	9	theme	versatile	1607:1615	arg1	paradigm					1628:1635	a versatile and robust paradigm	1605:1635	a versatile and robust paradigm for wound treatment	1605:1655	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	2	10	theme	healing	322:328	arg1	stages					306:311	different stages	296:311	different stages of wound healing	296:328	New material design satisfying all demands throughout different stages of wound healing is cherished but rarely discussed.					
36265178	8	11	theme	robust	1621:1626	arg1	paradigm					1628:1635	a versatile and robust paradigm	1605:1635	a versatile and robust paradigm for wound treatment	1605:1655	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	7	12	theme	wound	1369:1373	arg1	healing					1375:1381	wound healing	1369:1381	wound healing	1369:1381	Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing.					
36265178	6	13	theme	bactericidal	1027:1038	arg1	infections					1040:1049	bactericidal infections	1027:1049	bactericidal infections due to their amphiphilic and cationic surfaces	1027:1096	Moreover, the alkylated chitosan within the microcapsules exhibits persistent antimicrobial activities against bactericidal infections due to their amphiphilic and cationic surfaces.					
36265178	7	14	theme	subsequent	1270:1279	arg1	development					1281:1291	the subsequent development	1266:1291	the subsequent development of granulation tissue	1266:1313	Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing.					
36265178	2	15	theme	wound	316:320	arg1	healing					322:328	wound healing	316:328	wound healing	316:328	New material design satisfying all demands throughout different stages of wound healing is cherished but rarely discussed.					
36265178	4	16	theme	blood	811:815	arg1	absorption					797:806	fast absorption	792:806	fast absorption of blood and exudate	792:827	SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate.					
36265178	7	17	theme	last	1354:1357	arg1	stages					1359:1364	the last stages	1350:1364	the last stages of wound healing	1350:1381	Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing.					
36265178	8	18	theme	time-sequential	1462:1476	arg1	functions					1478:1486	time-sequential functions	1462:1486	time-sequential functions for tissue repairing	1462:1507	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	8	19	theme	soft	1428:1431	arg1	microcapsules					1433:1445	size-tailored soft microcapsules	1414:1445	size-tailored soft microcapsules	1414:1445	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	3	20	theme	wound	598:602	arg1	healing					604:610	wound healing	598:610	wound healing	598:610	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	4	21	theme	porous	762:767	arg1	cores					782:786	their internal porous dextran-rich cores	747:786	their internal porous dextran-rich cores for fast absorption of blood and exudate	747:827	SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate.					
36265178	3	22	theme	chitosan/alginate	495:511	arg1	materials					556:564	novel therapeutic materials	538:564	novel therapeutic materials for rapid blood coagulation and wound healing	538:610	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	22	theme	chitosan/alginate	495:511	arg1	SAAMs					528:532	SAAMs	528:532	SAAMs	528:532	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	22	theme	chitosan/alginate	495:511	arg1	microcapsules					513:525	self-assembled, multifunctional alkylated chitosan/alginate microcapsules	453:525	microcapsules	513:525	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	23	theme	all-aqueous	383:393	arg1	strategy					431:438	a novel strategy	423:438	a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing	423:610	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	23	theme	all-aqueous	383:393	arg1	microfluidics					406:418	all-aqueous multiphase microfluidics	383:418	all-aqueous multiphase microfluidics	383:418	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	24	theme	self-assembled	453:466	arg1	materials					556:564	novel therapeutic materials	538:564	novel therapeutic materials for rapid blood coagulation and wound healing	538:610	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	24	theme	self-assembled	453:466	arg1	SAAMs					528:532	SAAMs	528:532	SAAMs	528:532	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	24	theme	self-assembled	453:466	arg1	microcapsules					513:525	self-assembled, multifunctional alkylated chitosan/alginate microcapsules	453:525	microcapsules	513:525	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	8	25	dep	ability	1393:1399	arg1	fabricate					1404:1412	fabricate	1404:1412	to fabricate size-tailored soft microcapsules	1401:1445	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	8	25	dep	ability	1393:1399	arg1	realize					1454:1460	realize	1454:1460	to realize time-sequential functions for tissue repairing	1451:1507	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	1	26	theme	Wound	105:109	arg1	healing					111:117	Wound healing	105:117	Wound healing	105:117	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	1	27	theme	cell	199:202	arg1	proliferation					204:216	cell proliferation	199:216	cell proliferation	199:216	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	0	28	theme	Microfluidics	12:24	arg1	Fabrication					26:36	All-Aqueous Microfluidics Fabrication	0:36	All-Aqueous Microfluidics Fabrication of Multifunctional Bioactive Microcapsules	0:79	All-Aqueous Microfluidics Fabrication of Multifunctional Bioactive Microcapsules Promotes Wound Healing.					
36265178	6	29	theme	antimicrobial	994:1006	arg1	activities					1008:1017	persistent antimicrobial activities	983:1017	persistent antimicrobial activities against bactericidal infections due to their amphiphilic and cationic surfaces	983:1096	Moreover, the alkylated chitosan within the microcapsules exhibits persistent antimicrobial activities against bactericidal infections due to their amphiphilic and cationic surfaces.					
36265178	7	30	theme	epidermal	1238:1246	arg1	cells					1248:1252	epidermal cells	1238:1252	epidermal cells	1238:1252	Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing.					
36265178	3	31	theme	multifunctional	469:483	arg1	materials					556:564	novel therapeutic materials	538:564	novel therapeutic materials for rapid blood coagulation and wound healing	538:610	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	31	theme	multifunctional	469:483	arg1	SAAMs					528:532	SAAMs	528:532	SAAMs	528:532	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	31	theme	multifunctional	469:483	arg1	microcapsules					513:525	self-assembled, multifunctional alkylated chitosan/alginate microcapsules	453:525	microcapsules	513:525	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	0	32	theme	All-Aqueous	0:10	arg1	Fabrication					26:36	All-Aqueous Microfluidics Fabrication	0:36	All-Aqueous Microfluidics Fabrication of Multifunctional Bioactive Microcapsules	0:79	All-Aqueous Microfluidics Fabrication of Multifunctional Bioactive Microcapsules Promotes Wound Healing.					
36265178	7	33	theme	cells	1248:1252	arg1	proliferation					1221:1233	the proliferation	1217:1233	the proliferation of epidermal cells	1217:1252	Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing.					
36265178	4	34	theme	exudate	821:827	arg1	absorption					797:806	fast absorption	792:806	fast absorption of blood and exudate	792:827	SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate.					
36265178	3	35	theme	alkylated	485:493	arg1	materials					556:564	novel therapeutic materials	538:564	novel therapeutic materials for rapid blood coagulation and wound healing	538:610	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	35	theme	alkylated	485:493	arg1	SAAMs					528:532	SAAMs	528:532	SAAMs	528:532	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	35	theme	alkylated	485:493	arg1	microcapsules					513:525	self-assembled, multifunctional alkylated chitosan/alginate microcapsules	453:525	microcapsules	513:525	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	4	36	theme	polycationic	681:692	arg1	surface					694:700	polycationic surface	681:700	polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate	681:827	SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate.					
36265178	1	37	theme	multiple	128:135	arg1	proliferation					204:216	cell proliferation	199:216	cell proliferation	199:216	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	1	37	theme	multiple	128:135	arg1	hemostasis					173:182	hemostasis	173:182	hemostasis	173:182	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	1	37	theme	multiple	128:135	arg1	inflammation					185:196	inflammation	185:196	inflammation	185:196	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	1	37	theme	multiple	128:135	arg1	remodeling					230:239	tissue remodeling	223:239	tissue remodeling	223:239	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	1	37	theme	multiple	128:135	arg1	stages					137:142	multiple stages	128:142	multiple stages	128:142	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	7	38	theme	inflammation	1334:1345	arg1	suppression					1319:1329	suppression	1319:1329	suppression of inflammation	1319:1345	Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing.					
36265178	7	38	theme	inflammation	1334:1345	arg1	development					1281:1291	the subsequent development	1266:1291	the subsequent development of granulation tissue	1266:1313	Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing.					
36265178	8	39	theme	microfluidics	1537:1549	arg1	generation					1551:1560	the presented "all-aqueous microfluidics generation	1510:1560	the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs"	1510:1596	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	6	40	theme	persistent	983:992	arg1	activities					1008:1017	persistent antimicrobial activities	983:1017	persistent antimicrobial activities against bactericidal infections due to their amphiphilic and cationic surfaces	983:1096	Moreover, the alkylated chitosan within the microcapsules exhibits persistent antimicrobial activities against bactericidal infections due to their amphiphilic and cationic surfaces.					
36265178	8	41	theme	multifunctional	1565:1579	arg1	"					1596:1596	multifunctional bioactive SAAMs"	1565:1596	multifunctional bioactive SAAMs"	1565:1596	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	1	42	theme	tissue	223:228	arg1	remodeling					230:239	tissue remodeling	223:239	tissue remodeling	223:239	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	0	43	theme	Bioactive	57:65	arg1	Microcapsules					67:79	Multifunctional Bioactive Microcapsules	41:79	Multifunctional Bioactive Microcapsules	41:79	All-Aqueous Microfluidics Fabrication of Multifunctional Bioactive Microcapsules Promotes Wound Healing.					
36265178	8	44	theme	"	1596:1596	arg1	generation					1551:1560	the presented "all-aqueous microfluidics generation	1510:1560	the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs"	1510:1596	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	4	45	theme	internal	753:760	arg1	cores					782:786	their internal porous dextran-rich cores	747:786	their internal porous dextran-rich cores for fast absorption of blood and exudate	747:827	SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate.					
36265178	4	46	theme	cascade	735:741	arg1	activation					712:721	rapid activation	706:721	rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate	706:827	SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate.					
36265178	0	47	theme	Multifunctional	41:55	arg1	Microcapsules					67:79	Multifunctional Bioactive Microcapsules	41:79	Multifunctional Bioactive Microcapsules	41:79	All-Aqueous Microfluidics Fabrication of Multifunctional Bioactive Microcapsules Promotes Wound Healing.					
36265178	8	48	theme	SAAMs	1591:1595	arg1	"					1596:1596	multifunctional bioactive SAAMs"	1565:1596	multifunctional bioactive SAAMs"	1565:1596	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	7	49	theme	organic-solvent-free	1148:1167	arg1	microcapsules					1169:1181	the organic-solvent-free microcapsules	1144:1181	the organic-solvent-free microcapsules	1144:1181	Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing.					
36265178	4	50	theme	clotting	726:733	arg1	cascade					735:741	clotting cascade	726:741	clotting cascade	726:741	SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate.					
36265178	7	51	theme	granulation	1296:1306	arg1	tissue					1308:1313	granulation tissue	1296:1313	granulation tissue	1296:1313	Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing.					
36265178	6	52	theme	cationic	1080:1087	arg1	surfaces					1089:1096	their amphiphilic and cationic surfaces	1058:1096	their amphiphilic and cationic surfaces	1058:1096	Moreover, the alkylated chitosan within the microcapsules exhibits persistent antimicrobial activities against bactericidal infections due to their amphiphilic and cationic surfaces.					
36265178	5	53	theme	acute	898:902	arg1	hemorrhage					904:913	acute hemorrhage	898:913	acute hemorrhage	898:913	These features endow SAAMs with excellent hemostatic properties for acute hemorrhage.					
36265178	2	54	theme	material	246:253	arg1	design					255:260	New material design	242:260	New material design satisfying all demands throughout different stages of wound healing	242:328	New material design satisfying all demands throughout different stages of wound healing is cherished but rarely discussed.					
36265178	8	55	theme	tissue	1492:1497	arg1	repairing					1499:1507	tissue repairing	1492:1507	tissue repairing	1492:1507	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	0	56	theme	Microcapsules	67:79	arg1	Fabrication					26:36	All-Aqueous Microfluidics Fabrication	0:36	All-Aqueous Microfluidics Fabrication of Multifunctional Bioactive Microcapsules	0:79	All-Aqueous Microfluidics Fabrication of Multifunctional Bioactive Microcapsules Promotes Wound Healing.					
36265178	6	57	theme	due	1051:1053	arg1	infections					1040:1049	bactericidal infections	1027:1049	bactericidal infections due to their amphiphilic and cationic surfaces	1027:1096	Moreover, the alkylated chitosan within the microcapsules exhibits persistent antimicrobial activities against bactericidal infections due to their amphiphilic and cationic surfaces.					
36265178	2	58	theme	New	242:244	arg1	design					255:260	New material design	242:260	New material design satisfying all demands throughout different stages of wound healing	242:328	New material design satisfying all demands throughout different stages of wound healing is cherished but rarely discussed.					
36265178	1	59	theme	body	147:150	arg1	responses					152:160	body responses	147:160	body responses	147:160	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	6	60	theme	amphiphilic	1064:1074	arg1	surfaces					1089:1096	their amphiphilic and cationic surfaces	1058:1096	their amphiphilic and cationic surfaces	1058:1096	Moreover, the alkylated chitosan within the microcapsules exhibits persistent antimicrobial activities against bactericidal infections due to their amphiphilic and cationic surfaces.					
36265178	7	61	theme	tissue	1308:1313	arg1	suppression					1319:1329	suppression	1319:1329	suppression of inflammation	1319:1345	Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing.					
36265178	7	61	theme	tissue	1308:1313	arg1	development					1281:1291	the subsequent development	1266:1291	the subsequent development of granulation tissue	1266:1313	Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing.					
36265178	2	62	theme	different	296:304	arg1	stages					306:311	different stages	296:311	different stages of wound healing	296:328	New material design satisfying all demands throughout different stages of wound healing is cherished but rarely discussed.					
36265178	8	63	theme	size-tailored	1414:1426	arg1	microcapsules					1433:1445	size-tailored soft microcapsules	1414:1445	size-tailored soft microcapsules	1414:1445	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	1	64	theme	responses	152:160	arg1	proliferation					204:216	cell proliferation	199:216	cell proliferation	199:216	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	1	64	theme	responses	152:160	arg1	hemostasis					173:182	hemostasis	173:182	hemostasis	173:182	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	1	64	theme	responses	152:160	arg1	inflammation					185:196	inflammation	185:196	inflammation	185:196	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	1	64	theme	responses	152:160	arg1	remodeling					230:239	tissue remodeling	223:239	tissue remodeling	223:239	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	1	64	theme	responses	152:160	arg1	stages					137:142	multiple stages	128:142	multiple stages	128:142	Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling.					
36265178	8	65	theme	bioactive	1581:1589	arg1	"					1596:1596	multifunctional bioactive SAAMs"	1565:1596	multifunctional bioactive SAAMs"	1565:1596	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	8	66	theme	all-aqueous	1525:1535	arg1	generation					1551:1560	the presented "all-aqueous microfluidics generation	1510:1560	the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs"	1510:1596	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	6	67	theme	alkylated	930:938	arg1	chitosan					940:947	the alkylated chitosan	926:947	the alkylated chitosan within the microcapsules	926:972	Moreover, the alkylated chitosan within the microcapsules exhibits persistent antimicrobial activities against bactericidal infections due to their amphiphilic and cationic surfaces.					
36265178	3	68	dep	novel	538:542	arg1	therapeutic					544:554	therapeutic	544:554	therapeutic	544:554	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	4	69	theme	dextran-rich	769:780	arg1	cores					782:786	their internal porous dextran-rich cores	747:786	their internal porous dextran-rich cores for fast absorption of blood and exudate	747:827	SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate.					
36265178	8	70	theme	presented	1514:1522	arg1	generation					1551:1560	the presented "all-aqueous microfluidics generation	1510:1560	the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs"	1510:1596	With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.					
36265178	3	71	theme	novel	538:542	arg1	materials					556:564	novel therapeutic materials	538:564	novel therapeutic materials for rapid blood coagulation and wound healing	538:610	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	71	theme	novel	538:542	arg1	microcapsules					513:525	self-assembled, multifunctional alkylated chitosan/alginate microcapsules	453:525	microcapsules	513:525	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	4	72	theme	cores	782:786	arg1	activation					712:721	rapid activation	706:721	rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate	706:827	SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate.					
36265178	4	73	theme	rapid	706:710	arg1	activation					712:721	rapid activation	706:721	rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate	706:827	SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate.					
36265178	0	74	theme	Wound	90:94	arg1	Healing					96:102	Wound Healing	90:102	Wound Healing	90:102	All-Aqueous Microfluidics Fabrication of Multifunctional Bioactive Microcapsules Promotes Wound Healing.					
36265178	5	75	with	SAAMs	851:855	arg1	properties					883:892	excellent hemostatic properties	862:892	excellent hemostatic properties for acute hemorrhage	862:913	These features endow SAAMs with excellent hemostatic properties for acute hemorrhage.					
36265178	3	76	theme	novel	425:429	arg1	strategy					431:438	a novel strategy	423:438	a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing	423:610	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
36265178	3	76	theme	novel	425:429	arg1	microfluidics					406:418	all-aqueous multiphase microfluidics	383:418	all-aqueous multiphase microfluidics	383:418	Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing.					
37105288	6	0	theme	Si4+	864:867	arg1	species					869:875	Si4+ species	864:875	Si4+ species	864:875	XPS spectra demonstrate the presence of Si4+ species in RH, and Si3+/Si4+ tetrahedra in SCB and CS.					
37105288	6	1	dep	RH	880:881	arg1	tetrahedra					898:907	tetrahedra	898:907	tetrahedra	898:907	XPS spectra demonstrate the presence of Si4+ species in RH, and Si3+/Si4+ tetrahedra in SCB and CS.					
37105288	1	2	dep	management	107:116	arg1	The					103:105	The	103:105	The	103:105	The management and final disposal of agro-industrial wastes are one of the main environmental problems.					
37105288	4	3	theme	%	535:535	arg1	yields					520:525	Silica yields	513:525	Silica yields of 17.91%, 9.39%, and 3.25%	513:553	Silica yields of 17.91%, 9.39%, and 3.25% were obtained for RH, CS, and SCB.					
37105288	5	4	theme	textural	613:620	arg1	properties					622:631	the textural properties	609:631	the textural properties	609:631	On the other hand, the textural properties show that the siliceous materials exhibited mesoporous structures, with high silica composition in the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH.					
37105288	3	5	from	feedstocks	430:439	arg1	materials					407:415	silica materials	400:415	silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH)	400:510	This contribution was aimed to extract and characterize silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH).					
37105288	4	6	theme	%	542:542	arg1	yields					520:525	Silica yields	513:525	Silica yields of 17.91%, 9.39%, and 3.25%	513:553	Silica yields of 17.91%, 9.39%, and 3.25% were obtained for RH, CS, and SCB.					
37105288	5	7	from	composition	717:727	arg1	materials					736:744	the materials	732:744	the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH	732:821	On the other hand, the textural properties show that the siliceous materials exhibited mesoporous structures, with high silica composition in the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH.					
37105288	1	8	theme	wastes	156:161	arg1	management					107:116	management	107:116	management	107:116	The management and final disposal of agro-industrial wastes are one of the main environmental problems.					
37105288	1	8	theme	wastes	156:161	arg1	disposal					128:135	final disposal	122:135	final disposal	122:135	The management and final disposal of agro-industrial wastes are one of the main environmental problems.					
37105288	2	9	from	presence	218:225	arg1	by-products					258:268	some agricultural by-products	240:268	some agricultural by-products	240:268	Due to the presence of silica in some agricultural by-products, it is possible to convert waste into materials with advanced properties.					
37105288	0	10	link	Silica-derived	0:13	arg1	materials					15:23	Silica-derived materials	0:23	Silica-derived materials from agro-industrial waste biomass: Characterization and comparative studies.	0:101	Silica-derived materials from agro-industrial waste biomass: Characterization and comparative studies.					
37105288	1	11	theme	main	178:181	arg1	problems					197:204	the main environmental problems	174:204	the main environmental problems	174:204	The management and final disposal of agro-industrial wastes are one of the main environmental problems.					
37105288	2	12	with	materials	308:316	arg1	properties					332:341	advanced properties	323:341	advanced properties	323:341	Due to the presence of silica in some agricultural by-products, it is possible to convert waste into materials with advanced properties.					
37105288	0	13	from	biomass	52:58	arg1	materials					15:23	Silica-derived materials	0:23	Silica-derived materials from agro-industrial waste biomass: Characterization and comparative studies.	0:101	Silica-derived materials from agro-industrial waste biomass: Characterization and comparative studies.					
37105288	5	14	theme	mesoporous	677:686	arg1	structures					688:697	mesoporous structures	677:697	mesoporous structures	677:697	On the other hand, the textural properties show that the siliceous materials exhibited mesoporous structures, with high silica composition in the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH.					
37105288	3	15	theme	silica	400:405	arg1	materials					407:415	silica materials	400:415	silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH)	400:510	This contribution was aimed to extract and characterize silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH).					
37105288	1	16	theme	environmental	183:195	arg1	problems					197:204	the main environmental problems	174:204	the main environmental problems	174:204	The management and final disposal of agro-industrial wastes are one of the main environmental problems.					
37105288	5	17	theme	high	705:708	arg1	composition					717:727	high silica composition	705:727	high silica composition in the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH	705:821	On the other hand, the textural properties show that the siliceous materials exhibited mesoporous structures, with high silica composition in the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH.					
37105288	1	18	theme	problems	197:204	arg1	problems					197:204	the main environmental problems	174:204	the main environmental problems	174:204	The management and final disposal of agro-industrial wastes are one of the main environmental problems.					
37105288	1	18	theme	problems	197:204	arg1	one					167:169	one	167:169	one	167:169	The management and final disposal of agro-industrial wastes are one of the main environmental problems.					
37105288	0	19	theme	Silica-derived	0:13	arg1	materials					15:23	Silica-derived materials	0:23	Silica-derived materials from agro-industrial waste biomass: Characterization and comparative studies.	0:101	Silica-derived materials from agro-industrial waste biomass: Characterization and comparative studies.					
37105288	5	20	theme	silica	710:715	arg1	composition					717:727	high silica composition	705:727	high silica composition in the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH	705:821	On the other hand, the textural properties show that the siliceous materials exhibited mesoporous structures, with high silica composition in the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH.					
37105288	3	21	theme	sugarcane	451:459	arg1	bagasse					461:467	sugarcane bagasse	451:467	sugarcane bagasse (SCB)	451:473	This contribution was aimed to extract and characterize silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH).					
37105288	3	21	theme	sugarcane	451:459	arg1	SCB					470:472	SCB	470:472	SCB	470:472	This contribution was aimed to extract and characterize silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH).					
37105288	0	22	theme	agro-industrial	30:44	arg1	biomass					52:58	agro-industrial waste biomass	30:58	agro-industrial waste biomass	30:58	Silica-derived materials from agro-industrial waste biomass: Characterization and comparative studies.					
37105288	4	23	theme	Silica	513:518	arg1	yields					520:525	Silica yields	513:525	Silica yields of 17.91%, 9.39%, and 3.25%	513:553	Silica yields of 17.91%, 9.39%, and 3.25% were obtained for RH, CS, and SCB.					
37105288	5	24	theme	other	597:601	arg1	hand					603:606	the other hand	593:606	the other hand	593:606	On the other hand, the textural properties show that the siliceous materials exhibited mesoporous structures, with high silica composition in the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH.					
37105288	1	25	theme	final	122:126	arg1	disposal					128:135	final disposal	122:135	final disposal	122:135	The management and final disposal of agro-industrial wastes are one of the main environmental problems.					
37105288	3	26	theme	corn	476:479	arg1	CS					488:489	CS	488:489	CS	488:489	This contribution was aimed to extract and characterize silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH).					
37105288	3	26	theme	corn	476:479	arg1	stalk					481:485	corn stalk	476:485	corn stalk (CS)	476:490	This contribution was aimed to extract and characterize silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH).					
37105288	3	27	theme	various	422:428	arg1	bagasse					461:467	sugarcane bagasse	451:467	sugarcane bagasse (SCB)	451:473	This contribution was aimed to extract and characterize silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH).					
37105288	3	27	theme	various	422:428	arg1	stalk					481:485	corn stalk	476:485	corn stalk (CS)	476:490	This contribution was aimed to extract and characterize silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH).					
37105288	3	27	theme	various	422:428	arg1	husk					502:505	rice husk	497:505	rice husk (RH)	497:510	This contribution was aimed to extract and characterize silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH).					
37105288	3	27	theme	various	422:428	arg1	feedstocks					430:439	various feedstocks	422:439	various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH)	422:510	This contribution was aimed to extract and characterize silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH).					
37105288	5	28	theme	crystalline	770:780	arg1	SiO2					782:785	crystalline SiO2	770:785	crystalline SiO2 for SCB and CS and amorphous for RH	770:821	On the other hand, the textural properties show that the siliceous materials exhibited mesoporous structures, with high silica composition in the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH.					
37105288	5	29	theme	due	746:748	arg1	materials					736:744	the materials	732:744	the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH	732:821	On the other hand, the textural properties show that the siliceous materials exhibited mesoporous structures, with high silica composition in the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH.					
37105288	0	30	theme	waste	46:50	arg1	biomass					52:58	agro-industrial waste biomass	30:58	agro-industrial waste biomass	30:58	Silica-derived materials from agro-industrial waste biomass: Characterization and comparative studies.					
37105288	6	31	from	presence	852:859	arg1	RH					880:881	RH	880:881	RH	880:881	XPS spectra demonstrate the presence of Si4+ species in RH, and Si3+/Si4+ tetrahedra in SCB and CS.					
37105288	6	31	from	presence	852:859	arg1	Si3+/Si4+					888:896	Si3+/Si4+	888:896	Si3+/Si4+	888:896	XPS spectra demonstrate the presence of Si4+ species in RH, and Si3+/Si4+ tetrahedra in SCB and CS.					
37105288	4	32	theme	%	553:553	arg1	yields					520:525	Silica yields	513:525	Silica yields of 17.91%, 9.39%, and 3.25%	513:553	Silica yields of 17.91%, 9.39%, and 3.25% were obtained for RH, CS, and SCB.					
37105288	5	33	theme	SiO2	782:785	arg1	formation					757:765	the formation	753:765	the formation of crystalline SiO2 for SCB and CS and amorphous for RH	753:821	On the other hand, the textural properties show that the siliceous materials exhibited mesoporous structures, with high silica composition in the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH.					
37105288	2	34	theme	silica	230:235	arg1	presence					218:225	the presence	214:225	the presence of silica in some agricultural by-products	214:268	Due to the presence of silica in some agricultural by-products, it is possible to convert waste into materials with advanced properties.					
37105288	6	35	attach	presence	852:859	arg1	RH					880:881	RH	880:881	RH	880:881	XPS spectra demonstrate the presence of Si4+ species in RH, and Si3+/Si4+ tetrahedra in SCB and CS.					
37105288	6	35	attach	presence	852:859	arg1	Si3+/Si4+					888:896	Si3+/Si4+	888:896	Si3+/Si4+	888:896	XPS spectra demonstrate the presence of Si4+ species in RH, and Si3+/Si4+ tetrahedra in SCB and CS.					
37105288	6	35	attach	presence	852:859	arg2	species					869:875	Si4+ species	864:875	Si4+ species	864:875	XPS spectra demonstrate the presence of Si4+ species in RH, and Si3+/Si4+ tetrahedra in SCB and CS.					
37105288	1	36	theme	agro-industrial	140:154	arg1	wastes					156:161	agro-industrial wastes	140:161	agro-industrial wastes	140:161	The management and final disposal of agro-industrial wastes are one of the main environmental problems.					
37105288	6	37	theme	species	869:875	arg1	presence					852:859	the presence	848:859	the presence of Si4+ species in RH, and Si3+/Si4+ tetrahedra in SCB and CS	848:921	XPS spectra demonstrate the presence of Si4+ species in RH, and Si3+/Si4+ tetrahedra in SCB and CS.					
37105288	2	38	theme	advanced	323:330	arg1	properties					332:341	advanced properties	323:341	advanced properties	323:341	Due to the presence of silica in some agricultural by-products, it is possible to convert waste into materials with advanced properties.					
37105288	0	39	theme	comparative	82:92	arg1	studies					94:100	comparative studies	82:100	comparative studies	82:100	Silica-derived materials from agro-industrial waste biomass: Characterization and comparative studies.					
37105288	0	40	dep	materials	15:23	arg1	studies					94:100	comparative studies	82:100	comparative studies	82:100	Silica-derived materials from agro-industrial waste biomass: Characterization and comparative studies.					
37105288	0	40	dep	materials	15:23	arg1	Characterization					61:76	Characterization	61:76	Characterization	61:76	Silica-derived materials from agro-industrial waste biomass: Characterization and comparative studies.					
37105288	3	41	theme	rice	497:500	arg1	RH					508:509	RH	508:509	RH	508:509	This contribution was aimed to extract and characterize silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH).					
37105288	3	41	theme	rice	497:500	arg1	husk					502:505	rice husk	497:505	rice husk (RH)	497:510	This contribution was aimed to extract and characterize silica materials from various feedstocks including sugarcane bagasse (SCB), corn stalk (CS), and rice husk (RH).					
37105288	2	42	attach	presence	218:225	arg1	by-products					258:268	some agricultural by-products	240:268	some agricultural by-products	240:268	Due to the presence of silica in some agricultural by-products, it is possible to convert waste into materials with advanced properties.					
37105288	2	42	attach	presence	218:225	arg2	silica					230:235	silica	230:235	silica	230:235	Due to the presence of silica in some agricultural by-products, it is possible to convert waste into materials with advanced properties.					
37105288	4	43	theme	9.39	538:541	arg1	%					542:542	9.39%	538:542	9.39%	538:542	Silica yields of 17.91%, 9.39%, and 3.25% were obtained for RH, CS, and SCB.					
37105288	5	44	theme	siliceous	647:655	arg1	materials					657:665	the siliceous materials	643:665	the siliceous materials	643:665	On the other hand, the textural properties show that the siliceous materials exhibited mesoporous structures, with high silica composition in the materials due to the formation of crystalline SiO2 for SCB and CS and amorphous for RH.					
37105288	2	45	theme	agricultural	245:256	arg1	by-products					258:268	some agricultural by-products	240:268	some agricultural by-products	240:268	Due to the presence of silica in some agricultural by-products, it is possible to convert waste into materials with advanced properties.					
37105288	6	46	theme	XPS	824:826	arg1	spectra					828:834	XPS spectra	824:834	XPS spectra	824:834	XPS spectra demonstrate the presence of Si4+ species in RH, and Si3+/Si4+ tetrahedra in SCB and CS.					
37354575	2	0	theme	/polyethylene	479:491	arg1	imine					493:497	reduced graphene oxide (rGO)/polyethylene imine	451:497	reduced graphene oxide (rGO)/polyethylene imine (PEI)	451:503	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	0	theme	/polyethylene	479:491	arg1	PEI					500:502	PEI	500:502	PEI	500:502	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	1	from	content	567:573	arg1	products					608:615	leisure meat products	595:615	leisure meat products	595:615	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	2	theme	soy	364:366	arg1	sauce					368:372	soy sauce	364:372	soy sauce	364:372	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	3	3	theme	detection	802:810	arg1	0.01 µmol/L					813:823	0.01 µmol/L	813:823	0.01 µmol/L	813:823	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	3	3	theme	detection	802:810	arg1	range					760:764	a relatively wide linear range	735:764	a relatively wide linear range (0.01-100 µmol/L)	735:782	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	3	3	theme	detection	802:810	arg1	limit					793:797	a lower limit	785:797	a lower limit of detection (0.01 µmol/L)	785:824	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	3	3	theme	detection	802:810	arg1	0.01-100 µmol/L					767:781	0.01-100 µmol/L	767:781	0.01-100 µmol/L	767:781	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	3	3	theme	detection	802:810	arg1	%					872:872	94.80-112.20%	860:872	94.80-112.20%	860:872	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	3	3	theme	detection	802:810	arg1	rate					854:857	an acceptable recovery rate	831:857	an acceptable recovery rate (94.80-112.20%)	831:873	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	0	4	theme	capsaicinoids	71:83	arg1	content					60:66	the content	56:66	the content of capsaicinoids	56:83	A highly sensitive electrochemical sensor for detecting the content of capsaicinoids based on the synergistic catalysis of rGO/PEI-CNTs/β-CD.					
37354575	3	5	theme	electrochemical	647:661	arg1	sensor					663:668	the electrochemical sensor	643:668	the electrochemical sensor	643:668	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	5	6	from	contents	1124:1131	arg1	products					1166:1173	leisure meat products	1153:1173	leisure meat products	1153:1173	The sensor detected trace contents of capsaicinoids in leisure meat products, and thus, it might be considered for practical applications.					
37354575	1	7	theme	content	170:176	arg1	quantification					148:161	Rapid quantification	142:161	Rapid quantification of the content of capsaicinoids	142:193	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
37354575	5	8	theme	meat	1161:1164	arg1	products					1166:1173	leisure meat products	1153:1173	leisure meat products	1153:1173	The sensor detected trace contents of capsaicinoids in leisure meat products, and thus, it might be considered for practical applications.					
37354575	2	9	theme	carbon	507:512	arg1	CNTs					525:528	CNTs	525:528	CNTs	525:528	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	9	theme	carbon	507:512	arg1	nanotubes					514:522	carbon nanotubes	507:522	carbon nanotubes (CNTs)	507:529	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	3	10	theme	acceptable	834:843	arg1	%					872:872	94.80-112.20%	860:872	94.80-112.20%	860:872	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	3	10	theme	acceptable	834:843	arg1	rate					854:857	an acceptable recovery rate	831:857	an acceptable recovery rate (94.80-112.20%)	831:873	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	1	11	theme	quality	270:276	arg1	control					278:284	quality control	270:284	quality control	270:284	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
37354575	4	12	with	catalysis	995:1003	arg1	cCNTs					1017:1021	cCNTs	1017:1021	cCNTs	1017:1021	The sensor performed well and was effective mainly because of the three-dimensional stacking structure and synergistic catalysis of rGO with cCNTs and also due to the improved dispersion of the composite material by β-CD.					
37354575	1	13	theme	control	278:284	arg1	degree					220:225	the degree	216:225	the degree of spiciness, standardized production, and quality control of leisure meat products	216:309	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
37354575	2	14	from	products	608:615	arg1	content					567:573	the content	563:573	the content of capsaicinoids in leisure meat products	563:615	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	15	theme	leisure	595:601	arg1	products					608:615	leisure meat products	595:615	leisure meat products	595:615	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	16	theme	meat	603:606	arg1	products					608:615	leisure meat products	595:615	leisure meat products	595:615	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	4	17	theme	material	1080:1087	arg1	dispersion					1052:1061	the improved dispersion	1039:1061	the improved dispersion of the composite material by β-CD	1039:1095	The sensor performed well and was effective mainly because of the three-dimensional stacking structure and synergistic catalysis of rGO with cCNTs and also due to the improved dispersion of the composite material by β-CD.					
37354575	0	18	theme	synergistic	98:108	arg1	catalysis					110:118	the synergistic catalysis	94:118	the synergistic catalysis of rGO/PEI-CNTs/β-CD	94:139	A highly sensitive electrochemical sensor for detecting the content of capsaicinoids based on the synergistic catalysis of rGO/PEI-CNTs/β-CD.					
37354575	3	19	theme	sensitive	687:695	arg1	performance					697:707	highly sensitive performance	680:707	highly sensitive performance toward capsaicinoids	680:728	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	4	20	theme	stacking	960:967	arg1	structure					969:977	the three-dimensional stacking structure	938:977	the three-dimensional stacking structure	938:977	The sensor performed well and was effective mainly because of the three-dimensional stacking structure and synergistic catalysis of rGO with cCNTs and also due to the improved dispersion of the composite material by β-CD.					
37354575	1	21	theme	capsaicinoids	181:193	arg1	content					170:176	the content	166:176	the content of capsaicinoids	166:193	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
37354575	2	22	theme	reduced	451:457	arg1	imine					493:497	reduced graphene oxide (rGO)/polyethylene imine	451:497	reduced graphene oxide (rGO)/polyethylene imine (PEI)	451:503	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	22	theme	reduced	451:457	arg1	PEI					500:502	PEI	500:502	PEI	500:502	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	23	theme	capsaicinoids	578:590	arg1	content					567:573	the content	563:573	the content of capsaicinoids in leisure meat products	563:615	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	4	24	with	structure	969:977	arg1	cCNTs					1017:1021	cCNTs	1017:1021	cCNTs	1017:1021	The sensor performed well and was effective mainly because of the three-dimensional stacking structure and synergistic catalysis of rGO with cCNTs and also due to the improved dispersion of the composite material by β-CD.					
37354575	2	25	theme	meat	388:391	arg1	products					393:400	pot-roast meat products	378:400	pot-roast meat products	378:400	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	4	26	theme	synergistic	983:993	arg1	catalysis					995:1003	synergistic catalysis	983:1003	synergistic catalysis of rGO with cCNTs and also due to the improved dispersion of the composite material by β-CD	983:1095	The sensor performed well and was effective mainly because of the three-dimensional stacking structure and synergistic catalysis of rGO with cCNTs and also due to the improved dispersion of the composite material by β-CD.					
37354575	5	27	theme	leisure	1153:1159	arg1	products					1166:1173	leisure meat products	1153:1173	leisure meat products	1153:1173	The sensor detected trace contents of capsaicinoids in leisure meat products, and thus, it might be considered for practical applications.					
37354575	2	28	theme	pot-roast	378:386	arg1	products					393:400	pot-roast meat products	378:400	pot-roast meat products	378:400	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	0	29	theme	electrochemical	19:33	arg1	sensor					35:40	A highly sensitive electrochemical sensor	0:40	A highly sensitive electrochemical sensor for detecting the content of capsaicinoids	0:83	A highly sensitive electrochemical sensor for detecting the content of capsaicinoids based on the synergistic catalysis of rGO/PEI-CNTs/β-CD.					
37354575	1	30	theme	leisure	289:295	arg1	products					302:309	leisure meat products	289:309	leisure meat products	289:309	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
37354575	0	31	theme	rGO/PEI-CNTs/β-CD	123:139	arg1	catalysis					110:118	the synergistic catalysis	94:118	the synergistic catalysis of rGO/PEI-CNTs/β-CD	94:139	A highly sensitive electrochemical sensor for detecting the content of capsaicinoids based on the synergistic catalysis of rGO/PEI-CNTs/β-CD.					
37354575	2	32	theme	electrochemical	419:433	arg1	sensor					435:440	an electrochemical sensor	416:440	an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD)	416:551	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	0	33	theme	sensitive	9:17	arg1	sensor					35:40	A highly sensitive electrochemical sensor	0:40	A highly sensitive electrochemical sensor for detecting the content of capsaicinoids	0:83	A highly sensitive electrochemical sensor for detecting the content of capsaicinoids based on the synergistic catalysis of rGO/PEI-CNTs/β-CD.					
37354575	1	34	theme	meat	297:300	arg1	products					302:309	leisure meat products	289:309	leisure meat products	289:309	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
37354575	5	35	theme	capsaicinoids	1136:1148	arg1	contents					1124:1131	trace contents	1118:1131	trace contents of capsaicinoids in leisure meat products	1118:1173	The sensor detected trace contents of capsaicinoids in leisure meat products, and thus, it might be considered for practical applications.					
37354575	4	36	theme	composite	1070:1078	arg1	material					1080:1087	the composite material	1066:1087	the composite material by β-CD	1066:1095	The sensor performed well and was effective mainly because of the three-dimensional stacking structure and synergistic catalysis of rGO with cCNTs and also due to the improved dispersion of the composite material by β-CD.					
37354575	2	37	theme	rGO	475:477	arg1	imine					493:497	reduced graphene oxide (rGO)/polyethylene imine	451:497	reduced graphene oxide (rGO)/polyethylene imine (PEI)	451:503	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	37	theme	rGO	475:477	arg1	PEI					500:502	PEI	500:502	PEI	500:502	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	1	38	theme	products	302:309	arg1	spiciness					230:238	spiciness	230:238	spiciness	230:238	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
37354575	1	38	theme	products	302:309	arg1	production					254:263	standardized production	241:263	standardized production	241:263	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
37354575	1	38	theme	products	302:309	arg1	control					278:284	quality control	270:284	quality control	270:284	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
37354575	4	39	theme	three-dimensional	942:958	arg1	structure					969:977	the three-dimensional stacking structure	938:977	the three-dimensional stacking structure	938:977	The sensor performed well and was effective mainly because of the three-dimensional stacking structure and synergistic catalysis of rGO with cCNTs and also due to the improved dispersion of the composite material by β-CD.					
37354575	2	40	from	capsaicinoids	578:590	arg1	products					608:615	leisure meat products	595:615	leisure meat products	595:615	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	4	41	theme	rGO	1008:1010	arg1	structure					969:977	the three-dimensional stacking structure	938:977	the three-dimensional stacking structure	938:977	The sensor performed well and was effective mainly because of the three-dimensional stacking structure and synergistic catalysis of rGO with cCNTs and also due to the improved dispersion of the composite material by β-CD.					
37354575	4	41	theme	rGO	1008:1010	arg1	catalysis					995:1003	synergistic catalysis	983:1003	synergistic catalysis of rGO with cCNTs and also due to the improved dispersion of the composite material by β-CD	983:1095	The sensor performed well and was effective mainly because of the three-dimensional stacking structure and synergistic catalysis of rGO with cCNTs and also due to the improved dispersion of the composite material by β-CD.					
37354575	2	42	theme	oxide	468:472	arg1	imine					493:497	reduced graphene oxide (rGO)/polyethylene imine	451:497	reduced graphene oxide (rGO)/polyethylene imine (PEI)	451:503	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	42	theme	oxide	468:472	arg1	PEI					500:502	PEI	500:502	PEI	500:502	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	3	43	theme	wide	748:751	arg1	range					760:764	a relatively wide linear range	735:764	a relatively wide linear range (0.01-100 µmol/L)	735:782	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	3	43	theme	wide	748:751	arg1	0.01-100 µmol/L					767:781	0.01-100 µmol/L	767:781	0.01-100 µmol/L	767:781	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	2	44	theme	capsaicinoids	347:359	arg1	content					336:342	the content	332:342	the content of capsaicinoids	332:359	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	45	theme	graphene	459:466	arg1	imine					493:497	reduced graphene oxide (rGO)/polyethylene imine	451:497	reduced graphene oxide (rGO)/polyethylene imine (PEI)	451:503	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	45	theme	graphene	459:466	arg1	PEI					500:502	PEI	500:502	PEI	500:502	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	3	46	theme	linear	753:758	arg1	range					760:764	a relatively wide linear range	735:764	a relatively wide linear range (0.01-100 µmol/L)	735:782	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	3	46	theme	linear	753:758	arg1	0.01-100 µmol/L					767:781	0.01-100 µmol/L	767:781	0.01-100 µmol/L	767:781	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	5	47	theme	practical	1213:1221	arg1	applications					1223:1234	practical applications	1213:1234	practical applications	1213:1234	The sensor detected trace contents of capsaicinoids in leisure meat products, and thus, it might be considered for practical applications.					
37354575	5	48	theme	trace	1118:1122	arg1	contents					1124:1131	trace contents	1118:1131	trace contents of capsaicinoids in leisure meat products	1118:1173	The sensor detected trace contents of capsaicinoids in leisure meat products, and thus, it might be considered for practical applications.					
37354575	1	49	theme	spiciness	230:238	arg1	degree					220:225	the degree	216:225	the degree of spiciness, standardized production, and quality control of leisure meat products	216:309	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
37354575	2	50	dep	imine	493:497	arg1	β-CD					547:550	β-CD	547:550	β-CD	547:550	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	2	50	dep	imine	493:497	arg1	/β-cyclodextrin					530:544	/β-cyclodextrin	530:544	/β-cyclodextrin (β-CD)	530:551	To rapidly quantify the content of capsaicinoids in soy sauce and pot-roast meat products, we developed an electrochemical sensor based on reduced graphene oxide (rGO)/polyethylene imine (PEI) - carbon nanotubes (CNTs)/β-cyclodextrin (β-CD) to detect the content of capsaicinoids in leisure meat products.					
37354575	1	51	theme	Rapid	142:146	arg1	quantification					148:161	Rapid quantification	142:161	Rapid quantification of the content of capsaicinoids	142:193	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
37354575	4	52	theme	improved	1043:1050	arg1	dispersion					1052:1061	the improved dispersion	1039:1061	the improved dispersion of the composite material by β-CD	1039:1095	The sensor performed well and was effective mainly because of the three-dimensional stacking structure and synergistic catalysis of rGO with cCNTs and also due to the improved dispersion of the composite material by β-CD.					
37354575	3	53	theme	lower	787:791	arg1	0.01 µmol/L					813:823	0.01 µmol/L	813:823	0.01 µmol/L	813:823	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	3	53	theme	lower	787:791	arg1	limit					793:797	a lower limit	785:797	a lower limit of detection (0.01 µmol/L)	785:824	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	1	54	theme	standardized	241:252	arg1	production					254:263	standardized production	241:263	standardized production	241:263	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
37354575	3	55	theme	recovery	845:852	arg1	%					872:872	94.80-112.20%	860:872	94.80-112.20%	860:872	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	3	55	theme	recovery	845:852	arg1	rate					854:857	an acceptable recovery rate	831:857	an acceptable recovery rate (94.80-112.20%)	831:873	Our findings showed that the electrochemical sensor presented highly sensitive performance toward capsaicinoids with a relatively wide linear range (0.01-100 µmol/L), a lower limit of detection (0.01 µmol/L), and an acceptable recovery rate (94.80-112.20%).					
37354575	1	56	theme	production	254:263	arg1	degree					220:225	the degree	216:225	the degree of spiciness, standardized production, and quality control of leisure meat products	216:309	Rapid quantification of the content of capsaicinoids helps in classifying the degree of spiciness, standardized production, and quality control of leisure meat products.					
35500768	5	0	theme	Cu	1153:1154	arg1	ions					1160:1163	Cu(II) ions	1153:1163	Cu(II) ions	1153:1163	The adsorption mechanism was the electrostatic attraction, chelating effect and complex formation driving forces for the fast and efficient adsorption of Cu(II) ions.					
35500768	4	1	theme	water	755:759	arg1	stability					761:769	superior water stability	746:769	superior water stability	746:769	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	4	2	theme	cellulose	977:985	arg1	nanofibers					987:996	anionic polyacrylamide and carbonylated cellulose nanofibers	937:996	nanofibers	987:996	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	2	3	theme	traditional	343:353	arg1	adsorbents					355:364	the traditional adsorbents	339:364	the traditional adsorbents	339:364	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	4	4	theme	carbonylated	964:975	arg1	nanofibers					987:996	anionic polyacrylamide and carbonylated cellulose nanofibers	937:996	nanofibers	987:996	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	1	5	theme	ecological	239:248	arg1	environment					250:260	ecological environment	239:260	ecological environment	239:260	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	5	6	dep	attraction	1046:1055	arg1	forces					1105:1110	driving forces	1097:1110	driving forces	1097:1110	The adsorption mechanism was the electrostatic attraction, chelating effect and complex formation driving forces for the fast and efficient adsorption of Cu(II) ions.					
35500768	6	7	theme	removal	1170:1176	arg1	efficiency					1178:1187	The removal efficiency	1166:1187	The removal efficiency of the aerogels for Cu(II)	1166:1214	The removal efficiency of the aerogels for Cu(II) remained above 80% after 10 adsorption/regeneration cycles, suggesting its outstanding recyclability.					
35500768	1	8	theme	heavy	168:172	arg1	ions					180:183	heavy metal ions	168:183	heavy metal ions	168:183	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	0	9	theme	Cu	128:129	arg1	ions					135:138	highly efficient Cu(II) ions	111:138	highly efficient Cu(II) ions removal	111:146	Highly stable cellulose nanofiber/polyacrylamide aerogel via in-situ physical/chemical double crosslinking for highly efficient Cu(II) ions removal.					
35500768	7	10	theme	heavy	1407:1411	arg1	wastewater					1423:1432	heavy metal ion wastewater	1407:1432	heavy metal ion wastewater	1407:1432	The proposed aerogel adsorbent shows noteworthy potential for the practical treatment of heavy metal ion wastewater.					
35500768	3	11	theme	facile	609:614	arg1	cross-linking					649:661	facile in-situ physical/chemical double cross-linking	609:661	facile in-situ physical/chemical double cross-linking	609:661	The aerogel adsorbent was prepared by a two-step method via facile in-situ physical/chemical double cross-linking and freeze-drying processes.					
35500768	5	12	theme	fast	1120:1123	arg1	adsorption					1139:1148	the fast and efficient adsorption	1116:1148	the fast and efficient adsorption of Cu(II) ions	1116:1163	The adsorption mechanism was the electrostatic attraction, chelating effect and complex formation driving forces for the fast and efficient adsorption of Cu(II) ions.					
35500768	7	13	theme	practical	1384:1392	arg1	treatment					1394:1402	the practical treatment	1380:1402	the practical treatment of heavy metal ion wastewater	1380:1432	The proposed aerogel adsorbent shows noteworthy potential for the practical treatment of heavy metal ion wastewater.					
35500768	4	14	theme	thermal	727:733	arg1	stability					735:743	good thermal stability	722:743	good thermal stability	722:743	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	0	15	theme	efficient	118:126	arg1	Cu					128:129	highly efficient Cu	111:129	highly efficient Cu(II) ions removal	111:146	Highly stable cellulose nanofiber/polyacrylamide aerogel via in-situ physical/chemical double crosslinking for highly efficient Cu(II) ions removal.					
35500768	0	15	theme	efficient	118:126	arg1	II					131:132	II	131:132	II	131:132	Highly stable cellulose nanofiber/polyacrylamide aerogel via in-situ physical/chemical double crosslinking for highly efficient Cu(II) ions removal.					
35500768	4	16	theme	anionic	937:943	arg1	polyacrylamide					945:958	anionic polyacrylamide and carbonylated cellulose nanofibers	937:996	polyacrylamide	945:958	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	4	17	theme	adsorption	832:841	arg1	capacity					843:850	a maximum Langmuir adsorption capacity	813:850	a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers	813:996	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	1	18	theme	human	266:270	arg1	health					272:277	human health	266:277	human health	266:277	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	4	19	dep	240 mg g-1	874:883	arg1	up					868:869	up	868:869	up	868:869	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	4	20	theme	Cu	856:857	arg1	ions					863:866	Cu(II) ions	856:866	Cu(II) ions	856:866	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	7	21	theme	ion	1419:1421	arg1	wastewater					1423:1432	heavy metal ion wastewater	1407:1432	heavy metal ion wastewater	1407:1432	The proposed aerogel adsorbent shows noteworthy potential for the practical treatment of heavy metal ion wastewater.					
35500768	1	22	theme	metal	174:178	arg1	ions					180:183	heavy metal ions	168:183	heavy metal ions	168:183	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	4	23	theme	resulting	696:704	arg1	aerogels					706:713	The resulting aerogels	692:713	The resulting aerogels	692:713	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	2	24	theme	three-dimensional	379:395	arg1	aerogel					458:464	three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel	379:464	three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling	379:546	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	0	25	theme	stable	7:12	arg1	nanofiber/polyacrylamide					24:47	Highly stable cellulose nanofiber/polyacrylamide	0:47	Highly stable cellulose nanofiber/polyacrylamide	0:47	Highly stable cellulose nanofiber/polyacrylamide aerogel via in-situ physical/chemical double crosslinking for highly efficient Cu(II) ions removal.					
35500768	4	26	theme	physical/chemical	904:920	arg1	combination					922:932	the in-situ physical/chemical combination	892:932	the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers	892:996	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	7	27	theme	proposed	1322:1329	arg1	aerogel					1331:1337	The proposed aerogel adsorbent	1318:1347	The proposed aerogel adsorbent	1318:1347	The proposed aerogel adsorbent shows noteworthy potential for the practical treatment of heavy metal ion wastewater.					
35500768	5	28	theme	electrostatic	1032:1044	arg1	attraction					1046:1055	the electrostatic attraction	1028:1055	the electrostatic attraction	1028:1055	The adsorption mechanism was the electrostatic attraction, chelating effect and complex formation driving forces for the fast and efficient adsorption of Cu(II) ions.					
35500768	5	28	theme	electrostatic	1032:1044	arg1	mechanism					1014:1022	The adsorption mechanism	999:1022	The adsorption mechanism	999:1022	The adsorption mechanism was the electrostatic attraction, chelating effect and complex formation driving forces for the fast and efficient adsorption of Cu(II) ions.					
35500768	7	29	theme	adsorbent	1339:1347	arg1	aerogel					1331:1337	The proposed aerogel adsorbent	1318:1347	The proposed aerogel adsorbent	1318:1347	The proposed aerogel adsorbent shows noteworthy potential for the practical treatment of heavy metal ion wastewater.					
35500768	6	30	theme	aerogels	1196:1203	arg1	efficiency					1178:1187	The removal efficiency	1166:1187	The removal efficiency of the aerogels for Cu(II)	1166:1214	The removal efficiency of the aerogels for Cu(II) remained above 80% after 10 adsorption/regeneration cycles, suggesting its outstanding recyclability.					
35500768	2	31	theme	outstanding	471:481	arg1	adsorption					494:503	outstanding pollutants adsorption	471:503	outstanding pollutants adsorption	471:503	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	4	32	theme	in-situ	896:902	arg1	combination					922:932	the in-situ physical/chemical combination	892:932	the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers	892:996	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	2	33	dep	stability	308:316	arg1	the					304:306	the	304:306	the	304:306	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	2	34	theme	adsorbents	355:364	arg1	recyclability					322:334	recyclability	322:334	recyclability	322:334	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	2	34	theme	adsorbents	355:364	arg1	stability					308:316	stability	308:316	stability	308:316	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	0	35	theme	ions	135:138	arg1	removal					140:146	highly efficient Cu(II) ions removal	111:146	highly efficient Cu(II) ions removal	111:146	Highly stable cellulose nanofiber/polyacrylamide aerogel via in-situ physical/chemical double crosslinking for highly efficient Cu(II) ions removal.					
35500768	3	36	theme	two-step	589:596	arg1	method					598:603	a two-step method	587:603	a two-step method	587:603	The aerogel adsorbent was prepared by a two-step method via facile in-situ physical/chemical double cross-linking and freeze-drying processes.					
35500768	6	37	theme	outstanding	1291:1301	arg1	recyclability					1303:1315	its outstanding recyclability	1287:1315	its outstanding recyclability	1287:1315	The removal efficiency of the aerogels for Cu(II) remained above 80% after 10 adsorption/regeneration cycles, suggesting its outstanding recyclability.					
35500768	1	38	theme	global	190:195	arg1	concern					197:203	a global concern	188:203	a global concern due to detrimental effects on the ecological environment and human health	188:277	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	1	38	theme	global	190:195	arg1	pollution					155:163	Water pollution	149:163	Water pollution by heavy metal ions	149:183	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	0	39	theme	cellulose	14:22	arg1	nanofiber/polyacrylamide					24:47	Highly stable cellulose nanofiber/polyacrylamide	0:47	Highly stable cellulose nanofiber/polyacrylamide	0:47	Highly stable cellulose nanofiber/polyacrylamide aerogel via in-situ physical/chemical double crosslinking for highly efficient Cu(II) ions removal.					
35500768	4	40	theme	maximum	815:821	arg1	adsorption					832:841	a maximum Langmuir adsorption	813:841	a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers	813:996	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	2	41	theme	nanofiber/polyacrylamide	423:446	arg1	aerogel					458:464	three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel	379:464	three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling	379:546	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	4	42	theme	excellent	775:783	arg1	properties					796:805	excellent adsorption properties	775:805	excellent adsorption properties	775:805	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	1	43	theme	due	205:207	arg1	concern					197:203	a global concern	188:203	a global concern due to detrimental effects on the ecological environment and human health	188:277	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	1	43	theme	due	205:207	arg1	pollution					155:163	Water pollution	149:163	Water pollution by heavy metal ions	149:183	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	3	44	theme	double	642:647	arg1	cross-linking					649:661	facile in-situ physical/chemical double cross-linking	609:661	facile in-situ physical/chemical double cross-linking	609:661	The aerogel adsorbent was prepared by a two-step method via facile in-situ physical/chemical double cross-linking and freeze-drying processes.					
35500768	2	45	theme	cellulose	413:421	arg1	aerogel					458:464	three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel	379:464	three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling	379:546	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	2	46	theme	lamellar	397:404	arg1	aerogel					458:464	three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel	379:464	three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling	379:546	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	2	47	theme	easy	506:509	arg1	regeneration					511:522	easy regeneration	506:522	easy regeneration	506:522	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	5	48	theme	ions	1160:1163	arg1	adsorption					1139:1148	the fast and efficient adsorption	1116:1148	the fast and efficient adsorption of Cu(II) ions	1116:1163	The adsorption mechanism was the electrostatic attraction, chelating effect and complex formation driving forces for the fast and efficient adsorption of Cu(II) ions.					
35500768	2	49	theme	porous	406:411	arg1	aerogel					458:464	three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel	379:464	three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling	379:546	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	0	50	theme	physical/chemical	69:85	arg1	crosslinking					94:105	in-situ physical/chemical double crosslinking	61:105	in-situ physical/chemical double crosslinking for highly efficient Cu(II) ions removal	61:146	Highly stable cellulose nanofiber/polyacrylamide aerogel via in-situ physical/chemical double crosslinking for highly efficient Cu(II) ions removal.					
35500768	4	51	theme	polyacrylamide	945:958	arg1	combination					922:932	the in-situ physical/chemical combination	892:932	the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers	892:996	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	2	52	theme	multiple	529:536	arg1	recycling					538:546	multiple recycling	529:546	multiple recycling	529:546	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	3	53	theme	in-situ	616:622	arg1	cross-linking					649:661	facile in-situ physical/chemical double cross-linking	609:661	facile in-situ physical/chemical double cross-linking	609:661	The aerogel adsorbent was prepared by a two-step method via facile in-situ physical/chemical double cross-linking and freeze-drying processes.					
35500768	2	54	with	aerogel	458:464	arg1	recycling					538:546	multiple recycling	529:546	multiple recycling	529:546	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	2	54	with	aerogel	458:464	arg1	adsorption					494:503	outstanding pollutants adsorption	471:503	outstanding pollutants adsorption	471:503	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	2	54	with	aerogel	458:464	arg1	regeneration					511:522	easy regeneration	506:522	easy regeneration	506:522	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	4	55	theme	adsorption	785:794	arg1	properties					796:805	excellent adsorption properties	775:805	excellent adsorption properties	775:805	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	0	56	theme	in-situ	61:67	arg1	crosslinking					94:105	in-situ physical/chemical double crosslinking	61:105	in-situ physical/chemical double crosslinking for highly efficient Cu(II) ions removal	61:146	Highly stable cellulose nanofiber/polyacrylamide aerogel via in-situ physical/chemical double crosslinking for highly efficient Cu(II) ions removal.					
35500768	5	57	theme	efficient	1129:1137	arg1	adsorption					1139:1148	the fast and efficient adsorption	1116:1148	the fast and efficient adsorption of Cu(II) ions	1116:1163	The adsorption mechanism was the electrostatic attraction, chelating effect and complex formation driving forces for the fast and efficient adsorption of Cu(II) ions.					
35500768	3	58	theme	physical/chemical	624:640	arg1	cross-linking					649:661	facile in-situ physical/chemical double cross-linking	609:661	facile in-situ physical/chemical double cross-linking	609:661	The aerogel adsorbent was prepared by a two-step method via facile in-situ physical/chemical double cross-linking and freeze-drying processes.					
35500768	7	59	theme	metal	1413:1417	arg1	wastewater					1423:1432	heavy metal ion wastewater	1407:1432	heavy metal ion wastewater	1407:1432	The proposed aerogel adsorbent shows noteworthy potential for the practical treatment of heavy metal ion wastewater.					
35500768	2	60	theme	recyclability	322:334	arg1	problem					293:299	the problem	289:299	the problem of the stability and recyclability of the traditional adsorbents	289:364	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	7	61	theme	wastewater	1423:1432	arg1	treatment					1394:1402	the practical treatment	1380:1402	the practical treatment of heavy metal ion wastewater	1380:1432	The proposed aerogel adsorbent shows noteworthy potential for the practical treatment of heavy metal ion wastewater.					
35500768	4	62	theme	nanofibers	987:996	arg1	combination					922:932	the in-situ physical/chemical combination	892:932	the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers	892:996	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	5	63	theme	complex	1079:1085	arg1	formation					1087:1095	complex formation	1079:1095	complex formation	1079:1095	The adsorption mechanism was the electrostatic attraction, chelating effect and complex formation driving forces for the fast and efficient adsorption of Cu(II) ions.					
35500768	4	64	theme	superior	746:753	arg1	stability					761:769	superior water stability	746:769	superior water stability	746:769	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	5	65	theme	adsorption	1003:1012	arg1	attraction					1046:1055	the electrostatic attraction	1028:1055	the electrostatic attraction	1028:1055	The adsorption mechanism was the electrostatic attraction, chelating effect and complex formation driving forces for the fast and efficient adsorption of Cu(II) ions.					
35500768	5	65	theme	adsorption	1003:1012	arg1	mechanism					1014:1022	The adsorption mechanism	999:1022	The adsorption mechanism	999:1022	The adsorption mechanism was the electrostatic attraction, chelating effect and complex formation driving forces for the fast and efficient adsorption of Cu(II) ions.					
35500768	4	66	theme	Langmuir	823:830	arg1	adsorption					832:841	a maximum Langmuir adsorption	813:841	a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers	813:996	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	1	67	theme	detrimental	212:222	arg1	effects					224:230	detrimental effects	212:230	detrimental effects on the ecological environment and human health	212:277	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	1	68	from	effects	224:230	arg1	environment					250:260	ecological environment	239:260	ecological environment	239:260	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	1	68	from	effects	224:230	arg1	health					272:277	human health	266:277	human health	266:277	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	7	69	theme	noteworthy	1355:1364	arg1	potential					1366:1374	noteworthy potential	1355:1374	noteworthy potential for the practical treatment of heavy metal ion wastewater	1355:1432	The proposed aerogel adsorbent shows noteworthy potential for the practical treatment of heavy metal ion wastewater.					
35500768	6	70	theme	adsorption/regeneration	1244:1266	arg1	cycles					1268:1273	10 adsorption/regeneration cycles	1241:1273	10 adsorption/regeneration cycles	1241:1273	The removal efficiency of the aerogels for Cu(II) remained above 80% after 10 adsorption/regeneration cycles, suggesting its outstanding recyclability.					
35500768	2	71	theme	stability	308:316	arg1	problem					293:299	the problem	289:299	the problem of the stability and recyclability of the traditional adsorbents	289:364	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	1	72	dep	environment	250:260	arg1	the					235:237	the	235:237	the	235:237	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	5	73	theme	driving	1097:1103	arg1	forces					1105:1110	driving forces	1097:1110	driving forces	1097:1110	The adsorption mechanism was the electrostatic attraction, chelating effect and complex formation driving forces for the fast and efficient adsorption of Cu(II) ions.					
35500768	2	74	theme	composite	448:456	arg1	aerogel					458:464	three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel	379:464	three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling	379:546	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
35500768	1	75	theme	Water	149:153	arg1	pollution					155:163	Water pollution	149:163	Water pollution by heavy metal ions	149:183	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	1	75	theme	Water	149:153	arg1	concern					197:203	a global concern	188:203	a global concern due to detrimental effects on the ecological environment and human health	188:277	Water pollution by heavy metal ions is a global concern due to detrimental effects on the ecological environment and human health.					
35500768	4	76	theme	good	722:725	arg1	stability					735:743	good thermal stability	722:743	good thermal stability	722:743	The resulting aerogels showed good thermal stability, superior water stability and excellent adsorption properties, with a maximum Langmuir adsorption capacity for Cu(II) ions up to 240 mg g-1 due to the in-situ physical/chemical combination of anionic polyacrylamide and carbonylated cellulose nanofibers.					
35500768	0	77	theme	double	87:92	arg1	crosslinking					94:105	in-situ physical/chemical double crosslinking	61:105	in-situ physical/chemical double crosslinking for highly efficient Cu(II) ions removal	61:146	Highly stable cellulose nanofiber/polyacrylamide aerogel via in-situ physical/chemical double crosslinking for highly efficient Cu(II) ions removal.					
35500768	5	78	theme	chelating	1058:1066	arg1	effect					1068:1073	chelating effect	1058:1073	chelating effect	1058:1073	The adsorption mechanism was the electrostatic attraction, chelating effect and complex formation driving forces for the fast and efficient adsorption of Cu(II) ions.					
35500768	3	79	theme	freeze-drying	667:679	arg1	processes					681:689	freeze-drying processes	667:689	freeze-drying processes	667:689	The aerogel adsorbent was prepared by a two-step method via facile in-situ physical/chemical double cross-linking and freeze-drying processes.					
35500768	2	80	theme	pollutants	483:492	arg1	adsorption					494:503	outstanding pollutants adsorption	471:503	outstanding pollutants adsorption	471:503	To solve the problem of the stability and recyclability of the traditional adsorbents, we proposed three-dimensional lamellar porous cellulose nanofiber/polyacrylamide composite aerogel with outstanding pollutants adsorption, easy regeneration, and multiple recycling.					
36551250	9	0	theme	glycoprotein	1491:1502	arg1	receptor					1504:1511	its glycoprotein receptor	1487:1511	its glycoprotein receptor	1487:1511	We also provided structural elements that suggest that BoNT/F1 uses the strategy of BoNT/A1 combined with the strategy of botulinum neurotoxin type E to bind N-glycan of its glycoprotein receptor.					
36551250	10	1	theme	therapeutic	1753:1763	arg1	purposes					1765:1772	therapeutic purposes	1753:1772	therapeutic purposes	1753:1772	Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.					
36551250	3	2	theme	membrane	449:456	arg1	A					517:517	glycosylated human synaptic vesicle glycoprotein A	468:517	glycosylated human synaptic vesicle glycoprotein A (hSV2Ag)	468:526	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	2	theme	membrane	449:456	arg1	receptor					458:465	its membrane receptor	445:465	its membrane receptor	445:465	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	3	theme	synaptic	487:494	arg1	hSV2Ag					520:525	hSV2Ag	520:525	hSV2Ag	520:525	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	3	theme	synaptic	487:494	arg1	A					517:517	glycosylated human synaptic vesicle glycoprotein A	468:517	glycosylated human synaptic vesicle glycoprotein A (hSV2Ag)	468:526	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	3	theme	synaptic	487:494	arg1	receptor					458:465	its membrane receptor	445:465	its membrane receptor	445:465	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	4	4	theme	simulation	594:603	arg1	study					611:615	a molecular dynamics simulation (MDS) study	573:615	a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A	573:662	To elucidate these mechanisms, we performed a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A.					
36551250	3	5	theme	glycosylated	468:479	arg1	hSV2Ag					520:525	hSV2Ag	520:525	hSV2Ag	520:525	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	5	theme	glycosylated	468:479	arg1	A					517:517	glycosylated human synaptic vesicle glycoprotein A	468:517	glycosylated human synaptic vesicle glycoprotein A (hSV2Ag)	468:526	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	5	theme	glycosylated	468:479	arg1	receptor					458:465	its membrane receptor	445:465	its membrane receptor	445:465	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	10	6	gly	glycoprotein	1736:1747	arg1	glycoprotein					1736:1747	synaptic vesicle glycoprotein	1719:1747	synaptic vesicle glycoprotein for therapeutic purposes	1719:1772	Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.					
36551250	8	7	dep	residues	1213:1220	arg1	Y1132					1222:1226	Y1132	1222:1226	Y1132	1222:1226	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	8	7	dep	residues	1213:1220	arg1	K1134					1239:1243	K1134	1239:1243	K1134	1239:1243	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	8	7	dep	residues	1213:1220	arg1	residues					1213:1220	neurotoxin residues Y1132, Q1133 and K1134	1202:1243	neurotoxin residues Y1132, Q1133 and K1134	1202:1243	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	8	7	dep	residues	1213:1220	arg1	Q1133					1229:1233	Q1133	1229:1233	Q1133	1229:1233	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	6	8	with	interaction	852:862	arg1	SV2A					880:883	SV2A	880:883	SV2A	880:883	Our study suggested that interaction of BoNT/F1 with SV2A is exclusively mediated by N-glycan moiety of SV2A, which interacts with aromatic residues Y898, Y910, F946, Y1059 and H1273 of this toxin.					
36551250	3	9	theme	glycoprotein	504:515	arg1	hSV2Ag					520:525	hSV2Ag	520:525	hSV2Ag	520:525	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	9	theme	glycoprotein	504:515	arg1	A					517:517	glycosylated human synaptic vesicle glycoprotein A	468:517	glycosylated human synaptic vesicle glycoprotein A (hSV2Ag)	468:526	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	9	theme	glycoprotein	504:515	arg1	receptor					458:465	its membrane receptor	445:465	its membrane receptor	445:465	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	1	10	theme	microbial	171:179	arg1	neurotoxins					181:191	the deadliest microbial neurotoxins	157:191	the deadliest microbial neurotoxins in humans	157:201	Botulinum neurotoxins are the deadliest microbial neurotoxins in humans, with a lethal dose of 1 ng/kg.					
36551250	1	10	theme	microbial	171:179	arg1	neurotoxins					141:151	Botulinum neurotoxins	131:151	Botulinum neurotoxins	131:151	Botulinum neurotoxins are the deadliest microbial neurotoxins in humans, with a lethal dose of 1 ng/kg.					
36551250	8	11	theme	intermolecular	1286:1299	arg1	bonds					1310:1314	long-lasting intermolecular hydrogen bonds	1273:1314	long-lasting intermolecular hydrogen bonds	1273:1314	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	4	12	theme	molecular	575:583	arg1	simulation					594:603	a molecular dynamics simulation	573:603	a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A	573:662	To elucidate these mechanisms, we performed a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A.					
36551250	4	12	theme	molecular	575:583	arg1	MDS					606:608	MDS	606:608	MDS	606:608	To elucidate these mechanisms, we performed a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A.					
36551250	10	13	theme	BoNT/F1	1659:1665	arg1	protein					1667:1673	a BoNT/F1 protein	1657:1673	a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes	1657:1772	Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.					
36551250	10	13	theme	BoNT/F1	1659:1665	arg1	able					1687:1690	able	1687:1690	able	1687:1690	Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.					
36551250	1	14	from	neurotoxins	181:191	arg1	humans					196:201	humans	196:201	humans	196:201	Botulinum neurotoxins are the deadliest microbial neurotoxins in humans, with a lethal dose of 1 ng/kg.					
36551250	9	15	theme	structural	1334:1343	arg1	elements					1345:1352	structural elements	1334:1352	structural elements that suggest that BoNT/F1 uses the strategy of BoNT/A1 combined with the strategy of botulinum neurotoxin type E to bind N-glycan of its glycoprotein receptor	1334:1511	We also provided structural elements that suggest that BoNT/F1 uses the strategy of BoNT/A1 combined with the strategy of botulinum neurotoxin type E to bind N-glycan of its glycoprotein receptor.					
36551250	8	16	theme	bonds	1310:1314	arg1	formation					1260:1268	formation	1260:1268	formation of long-lasting intermolecular hydrogen bonds	1260:1314	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	4	17	theme	kinetics	636:643	arg1	study					611:615	a molecular dynamics simulation (MDS) study	573:615	a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A	573:662	To elucidate these mechanisms, we performed a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A.					
36551250	6	18	theme	toxin	1018:1022	arg1	residues					967:974	aromatic residues	958:974	aromatic residues	958:974	Our study suggested that interaction of BoNT/F1 with SV2A is exclusively mediated by N-glycan moiety of SV2A, which interacts with aromatic residues Y898, Y910, F946, Y1059 and H1273 of this toxin.					
36551250	6	18	theme	toxin	1018:1022	arg1	toxin					1018:1022	this toxin	1013:1022	this toxin	1013:1022	Our study suggested that interaction of BoNT/F1 with SV2A is exclusively mediated by N-glycan moiety of SV2A, which interacts with aromatic residues Y898, Y910, F946, Y1059 and H1273 of this toxin.					
36551250	5	19	theme	gangliosides	813:824	arg1	presence					787:794	the presence	783:794	the presence of both SV2A and gangliosides	783:824	Since this toxin also interacts with gangliosides, the simulations were performed at the periphery of a lipid raft in the presence of both SV2A and gangliosides.					
36551250	4	20	theme	BoNT/F1	648:654	arg1	kinetics					636:643	initial binding kinetics	620:643	initial binding kinetics of BoNT/F1 to SV2A	620:662	To elucidate these mechanisms, we performed a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A.					
36551250	0	21	theme	Raft	125:128	arg1	Periphery					104:112	the Periphery	100:112	the Periphery of a Lipid Raft	100:128	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	10	22	theme	N-glycan-toxin	1603:1616	arg1	interactions					1618:1629	N-glycan-toxin interactions	1603:1629	N-glycan-toxin interactions	1603:1629	Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.					
36551250	3	23	gly	glycoprotein	504:515	arg1	glycoprotein					504:515	glycosylated human synaptic vesicle glycoprotein A	468:517	glycosylated human synaptic vesicle glycoprotein A (hSV2Ag)	468:526	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	6	24	theme	N-glycan	912:919	arg1	moiety					921:926	N-glycan moiety	912:926	N-glycan moiety of SV2A, which interacts with aromatic residues Y898, Y910, F946, Y1059 and H1273 of this toxin	912:1022	Our study suggested that interaction of BoNT/F1 with SV2A is exclusively mediated by N-glycan moiety of SV2A, which interacts with aromatic residues Y898, Y910, F946, Y1059 and H1273 of this toxin.					
36551250	4	25	theme	initial	620:626	arg1	kinetics					636:643	initial binding kinetics	620:643	initial binding kinetics of BoNT/F1 to SV2A	620:662	To elucidate these mechanisms, we performed a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A.					
36551250	0	26	theme	Human	67:71	arg1	SV2A					73:76	Glycosylated Human SV2A	54:76	Glycosylated Human SV2A	54:76	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	5	27	theme	SV2A	804:807	arg1	presence					787:794	the presence	783:794	the presence of both SV2A and gangliosides	783:824	Since this toxin also interacts with gangliosides, the simulations were performed at the periphery of a lipid raft in the presence of both SV2A and gangliosides.					
36551250	9	28	theme	BoNT/A1	1401:1407	arg1	strategy					1389:1396	the strategy	1385:1396	the strategy of BoNT/A1 combined with the strategy of botulinum neurotoxin type E	1385:1465	We also provided structural elements that suggest that BoNT/F1 uses the strategy of BoNT/A1 combined with the strategy of botulinum neurotoxin type E to bind N-glycan of its glycoprotein receptor.					
36551250	8	29	theme	neurotoxin	1202:1211	arg1	Y1132					1222:1226	Y1132	1222:1226	Y1132	1222:1226	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	8	29	theme	neurotoxin	1202:1211	arg1	K1134					1239:1243	K1134	1239:1243	K1134	1239:1243	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	8	29	theme	neurotoxin	1202:1211	arg1	residues					1213:1220	neurotoxin residues Y1132, Q1133 and K1134	1202:1243	neurotoxin residues Y1132, Q1133 and K1134	1202:1243	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	8	29	theme	neurotoxin	1202:1211	arg1	Q1133					1229:1233	Q1133	1229:1233	Q1133	1229:1233	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	2	30	used	used	283:286	arg2	neurotoxins					255:265	these neurotoxins	249:265	these neurotoxins	249:265	Incidentally, these neurotoxins are also widely used for medical and cosmetic purposes.					
36551250	6	31	dep	residues	967:974	arg1	Y898					976:979	Y898	976:979	Y898	976:979	Our study suggested that interaction of BoNT/F1 with SV2A is exclusively mediated by N-glycan moiety of SV2A, which interacts with aromatic residues Y898, Y910, F946, Y1059 and H1273 of this toxin.					
36551250	0	32	theme	Structural	0:9	arg1	Basis					11:15	Structural Basis	0:15	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.	0:129	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	1	33	theme	ng/kg	228:232	arg1	dose					218:221	a lethal dose	209:221	a lethal dose of 1 ng/kg	209:232	Botulinum neurotoxins are the deadliest microbial neurotoxins in humans, with a lethal dose of 1 ng/kg.					
36551250	6	34	theme	aromatic	958:965	arg1	residues					967:974	aromatic residues	958:974	aromatic residues	958:974	Our study suggested that interaction of BoNT/F1 with SV2A is exclusively mediated by N-glycan moiety of SV2A, which interacts with aromatic residues Y898, Y910, F946, Y1059 and H1273 of this toxin.					
36551250	6	34	theme	aromatic	958:965	arg1	toxin					1018:1022	this toxin	1013:1022	this toxin	1013:1022	Our study suggested that interaction of BoNT/F1 with SV2A is exclusively mediated by N-glycan moiety of SV2A, which interacts with aromatic residues Y898, Y910, F946, Y1059 and H1273 of this toxin.					
36551250	3	35	theme	neurotoxin	413:422	arg1	binding					392:398	binding	392:398	binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag)	392:526	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	9	36	theme	botulinum	1439:1447	arg1	E					1465:1465	botulinum neurotoxin type E	1439:1465	botulinum neurotoxin type E	1439:1465	We also provided structural elements that suggest that BoNT/F1 uses the strategy of BoNT/A1 combined with the strategy of botulinum neurotoxin type E to bind N-glycan of its glycoprotein receptor.					
36551250	10	37	theme	protein	1700:1706	arg1	content					1708:1714	protein content	1700:1714	protein content of synaptic vesicle glycoprotein for therapeutic purposes	1700:1772	Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.					
36551250	1	38	theme	Botulinum	131:139	arg1	neurotoxins					181:191	the deadliest microbial neurotoxins	157:191	the deadliest microbial neurotoxins in humans	157:201	Botulinum neurotoxins are the deadliest microbial neurotoxins in humans, with a lethal dose of 1 ng/kg.					
36551250	1	38	theme	Botulinum	131:139	arg1	neurotoxins					141:151	Botulinum neurotoxins	131:151	Botulinum neurotoxins	131:151	Botulinum neurotoxins are the deadliest microbial neurotoxins in humans, with a lethal dose of 1 ng/kg.					
36551250	0	39	theme	Toxin	30:34	arg1	Binding					43:49	Botulinum Toxin Type F Binding	20:49	Botulinum Toxin Type F Binding	20:49	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	9	40	theme	type	1460:1463	arg1	E					1465:1465	botulinum neurotoxin type E	1439:1465	botulinum neurotoxin type E	1439:1465	We also provided structural elements that suggest that BoNT/F1 uses the strategy of BoNT/A1 combined with the strategy of botulinum neurotoxin type E to bind N-glycan of its glycoprotein receptor.					
36551250	2	41	theme	medical	292:298	arg1	purposes					313:320	medical and cosmetic purposes	292:320	medical and cosmetic purposes	292:320	Incidentally, these neurotoxins are also widely used for medical and cosmetic purposes.					
36551250	0	42	theme	F	41:41	arg1	Binding					43:49	Botulinum Toxin Type F Binding	20:49	Botulinum Toxin Type F Binding	20:49	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	7	43	theme	SV2A	1133:1136	arg1	content					1122:1128	protein content	1114:1128	protein content of SV2A	1114:1136	Thus, in contrast with botulinum neurotoxin A1 (BoNT/A1), BoNT/F1 does not interact with protein content of SV2A.					
36551250	10	44	theme	vesicle	1728:1734	arg1	glycoprotein					1736:1747	synaptic vesicle glycoprotein	1719:1747	synaptic vesicle glycoprotein for therapeutic purposes	1719:1772	Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.					
36551250	9	45	gly	glycoprotein	1491:1502	arg1	glycoprotein					1491:1502	its glycoprotein receptor	1487:1511	its glycoprotein receptor	1487:1511	We also provided structural elements that suggest that BoNT/F1 uses the strategy of BoNT/A1 combined with the strategy of botulinum neurotoxin type E to bind N-glycan of its glycoprotein receptor.					
36551250	0	46	from	Periphery	104:112	arg1	Studies					89:95	In Silico Studies	79:95	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.	0:129	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	8	47	theme	long-lasting	1273:1284	arg1	bonds					1310:1314	long-lasting intermolecular hydrogen bonds	1273:1314	long-lasting intermolecular hydrogen bonds	1273:1314	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	10	48	theme	inhibitor	1573:1581	arg1	design					1551:1556	design	1551:1556	design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions	1551:1629	Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.					
36551250	5	49	theme	raft	775:778	arg1	periphery					754:762	the periphery	750:762	the periphery of a lipid raft	750:778	Since this toxin also interacts with gangliosides, the simulations were performed at the periphery of a lipid raft in the presence of both SV2A and gangliosides.					
36551250	2	50	theme	cosmetic	304:311	arg1	purposes					313:320	medical and cosmetic purposes	292:320	medical and cosmetic purposes	292:320	Incidentally, these neurotoxins are also widely used for medical and cosmetic purposes.					
36551250	1	51	theme	lethal	211:216	arg1	dose					218:221	a lethal dose	209:221	a lethal dose of 1 ng/kg	209:232	Botulinum neurotoxins are the deadliest microbial neurotoxins in humans, with a lethal dose of 1 ng/kg.					
36551250	9	52	theme	receptor	1504:1511	arg1	N-glycan					1475:1482	N-glycan	1475:1482	N-glycan of its glycoprotein receptor	1475:1511	We also provided structural elements that suggest that BoNT/F1 uses the strategy of BoNT/A1 combined with the strategy of botulinum neurotoxin type E to bind N-glycan of its glycoprotein receptor.					
36551250	3	53	theme	human	481:485	arg1	hSV2Ag					520:525	hSV2Ag	520:525	hSV2Ag	520:525	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	53	theme	human	481:485	arg1	A					517:517	glycosylated human synaptic vesicle glycoprotein A	468:517	glycosylated human synaptic vesicle glycoprotein A (hSV2Ag)	468:526	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	53	theme	human	481:485	arg1	receptor					458:465	its membrane receptor	445:465	its membrane receptor	445:465	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	54	gly	glycosylated	468:479	arg1	hSV2Ag					520:525	hSV2Ag	520:525	hSV2Ag	520:525	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	54	gly	glycosylated	468:479	arg1	A					517:517	glycosylated human synaptic vesicle glycoprotein A	468:517	glycosylated human synaptic vesicle glycoprotein A (hSV2Ag)	468:526	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	54	gly	glycosylated	468:479	arg1	receptor					458:465	its membrane receptor	445:465	its membrane receptor	445:465	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	5	55	theme	lipid	769:773	arg1	raft					775:778	a lipid raft	767:778	a lipid raft	767:778	Since this toxin also interacts with gangliosides, the simulations were performed at the periphery of a lipid raft in the presence of both SV2A and gangliosides.					
36551250	4	56	theme	dynamics	585:592	arg1	simulation					594:603	a molecular dynamics simulation	573:603	a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A	573:662	To elucidate these mechanisms, we performed a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A.					
36551250	4	56	theme	dynamics	585:592	arg1	MDS					606:608	MDS	606:608	MDS	606:608	To elucidate these mechanisms, we performed a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A.					
36551250	3	57	theme	vesicle	496:502	arg1	hSV2Ag					520:525	hSV2Ag	520:525	hSV2Ag	520:525	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	57	theme	vesicle	496:502	arg1	A					517:517	glycosylated human synaptic vesicle glycoprotein A	468:517	glycosylated human synaptic vesicle glycoprotein A (hSV2Ag)	468:526	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	57	theme	vesicle	496:502	arg1	receptor					458:465	its membrane receptor	445:465	its membrane receptor	445:465	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	1	58	theme	deadliest	161:169	arg1	neurotoxins					181:191	the deadliest microbial neurotoxins	157:191	the deadliest microbial neurotoxins in humans	157:201	Botulinum neurotoxins are the deadliest microbial neurotoxins in humans, with a lethal dose of 1 ng/kg.					
36551250	1	58	theme	deadliest	161:169	arg1	neurotoxins					141:151	Botulinum neurotoxins	131:151	Botulinum neurotoxins	131:151	Botulinum neurotoxins are the deadliest microbial neurotoxins in humans, with a lethal dose of 1 ng/kg.					
36551250	6	59	theme	BoNT/F1	867:873	arg1	interaction					852:862	interaction	852:862	interaction of BoNT/F1 with SV2A	852:883	Our study suggested that interaction of BoNT/F1 with SV2A is exclusively mediated by N-glycan moiety of SV2A, which interacts with aromatic residues Y898, Y910, F946, Y1059 and H1273 of this toxin.					
36551250	8	60	theme	hydrogen	1301:1308	arg1	bonds					1310:1314	long-lasting intermolecular hydrogen bonds	1273:1314	long-lasting intermolecular hydrogen bonds	1273:1314	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	10	61	theme	protein	1667:1673	arg1	bioengineering					1639:1652	bioengineering	1639:1652	bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes	1639:1772	Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.					
36551250	0	62	theme	Lipid	119:123	arg1	Raft					125:128	a Lipid Raft	117:128	a Lipid Raft	117:128	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	4	63	theme	binding	628:634	arg1	kinetics					636:643	initial binding kinetics	620:643	initial binding kinetics of BoNT/F1 to SV2A	620:662	To elucidate these mechanisms, we performed a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A.					
36551250	7	64	with	contrast	1034:1041	arg1	neurotoxin					1058:1067	botulinum neurotoxin A1 (BoNT/A1)	1048:1080	botulinum neurotoxin A1 (BoNT/A1)	1048:1080	Thus, in contrast with botulinum neurotoxin A1 (BoNT/A1), BoNT/F1 does not interact with protein content of SV2A.					
36551250	6	65	theme	SV2A	931:934	arg1	moiety					921:926	N-glycan moiety	912:926	N-glycan moiety of SV2A, which interacts with aromatic residues Y898, Y910, F946, Y1059 and H1273 of this toxin	912:1022	Our study suggested that interaction of BoNT/F1 with SV2A is exclusively mediated by N-glycan moiety of SV2A, which interacts with aromatic residues Y898, Y910, F946, Y1059 and H1273 of this toxin.					
36551250	0	66	theme	Glycosylated	54:65	arg1	SV2A					73:76	Glycosylated Human SV2A	54:76	Glycosylated Human SV2A	54:76	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	0	67	theme	In	79:80	arg1	Studies					89:95	In Silico Studies	79:95	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.	0:129	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	7	68	theme	botulinum	1048:1056	arg1	neurotoxin					1058:1067	botulinum neurotoxin A1 (BoNT/A1)	1048:1080	botulinum neurotoxin A1 (BoNT/A1)	1048:1080	Thus, in contrast with botulinum neurotoxin A1 (BoNT/A1), BoNT/F1 does not interact with protein content of SV2A.					
36551250	0	69	dep	In	79:80	arg1	Silico					82:87	Silico	82:87	Silico	82:87	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	0	70	theme	Botulinum	20:28	arg1	Binding					43:49	Botulinum Toxin Type F Binding	20:49	Botulinum Toxin Type F Binding	20:49	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	0	71	dep	Basis	11:15	arg1	Studies					89:95	In Silico Studies	79:95	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.	0:129	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	1	72	with	neurotoxins	181:191	arg1	dose					218:221	a lethal dose	209:221	a lethal dose of 1 ng/kg	209:232	Botulinum neurotoxins are the deadliest microbial neurotoxins in humans, with a lethal dose of 1 ng/kg.					
36551250	3	73	theme	type	424:427	arg1	BoNT/F1					433:439	BoNT/F1	433:439	BoNT/F1	433:439	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	73	theme	type	424:427	arg1	F1					429:430	type F1	424:430	botulinum neurotoxin type F1 (BoNT/F1)	403:440	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	0	74	theme	Type	36:39	arg1	Binding					43:49	Botulinum Toxin Type F Binding	20:49	Botulinum Toxin Type F Binding	20:49	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	9	75	theme	neurotoxin	1449:1458	arg1	E					1465:1465	botulinum neurotoxin type E	1439:1465	botulinum neurotoxin type E	1439:1465	We also provided structural elements that suggest that BoNT/F1 uses the strategy of BoNT/A1 combined with the strategy of botulinum neurotoxin type E to bind N-glycan of its glycoprotein receptor.					
36551250	3	76	theme	molecular	358:366	arg1	mechanisms					368:377	the molecular mechanisms	354:377	the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag)	354:526	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	77	theme	botulinum	403:411	arg1	neurotoxin					413:422	botulinum neurotoxin type F1 (BoNT/F1)	403:440	botulinum neurotoxin type F1 (BoNT/F1)	403:440	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	0	78	theme	Binding	43:49	arg1	Basis					11:15	Structural Basis	0:15	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.	0:129	Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft.					
36551250	9	79	theme	E	1465:1465	arg1	strategy					1427:1434	the strategy	1423:1434	the strategy of botulinum neurotoxin type E	1423:1465	We also provided structural elements that suggest that BoNT/F1 uses the strategy of BoNT/A1 combined with the strategy of botulinum neurotoxin type E to bind N-glycan of its glycoprotein receptor.					
36551250	7	80	dep	neurotoxin	1058:1067	arg1	A1					1069:1070	A1	1069:1070	botulinum neurotoxin A1 (BoNT/A1)	1048:1080	Thus, in contrast with botulinum neurotoxin A1 (BoNT/A1), BoNT/F1 does not interact with protein content of SV2A.					
36551250	7	80	dep	neurotoxin	1058:1067	arg1	BoNT/A1					1073:1079	BoNT/A1	1073:1079	BoNT/A1	1073:1079	Thus, in contrast with botulinum neurotoxin A1 (BoNT/A1), BoNT/F1 does not interact with protein content of SV2A.					
36551250	10	81	theme	glycoprotein	1736:1747	arg1	content					1708:1714	protein content	1700:1714	protein content of synaptic vesicle glycoprotein for therapeutic purposes	1700:1772	Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.					
36551250	7	82	theme	protein	1114:1120	arg1	content					1122:1128	protein content	1114:1128	protein content of SV2A	1114:1136	Thus, in contrast with botulinum neurotoxin A1 (BoNT/A1), BoNT/F1 does not interact with protein content of SV2A.					
36551250	10	83	theme	synaptic	1719:1726	arg1	glycoprotein					1736:1747	synaptic vesicle glycoprotein	1719:1747	synaptic vesicle glycoprotein for therapeutic purposes	1719:1772	Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.					
36551250	8	84	theme	barrage	1176:1182	arg1	effect					1184:1189	a barrage effect	1174:1189	a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds	1174:1314	We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds.					
36551250	3	85	dep	neurotoxin	413:422	arg1	BoNT/F1					433:439	BoNT/F1	433:439	BoNT/F1	433:439	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	3	85	dep	neurotoxin	413:422	arg1	F1					429:430	type F1	424:430	botulinum neurotoxin type F1 (BoNT/F1)	403:440	However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag).					
36551250	10	86	theme	universal	1563:1571	arg1	inhibitor					1573:1581	a universal inhibitor	1561:1581	a universal inhibitor aimed at disrupting N-glycan-toxin interactions	1561:1629	Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan-toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.					
35872307	0	0	theme	ultralight	117:126	arg1	property					128:135	ultralight property	117:135	ultralight property	117:135	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	2	1	dep	removal	495:501	arg1	VI					491:492	VI	491:492	VI	491:492	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	5	2	theme	mono-layer	870:879	arg1	sorption					893:900	mono-layer homogeneous sorption	870:900	mono-layer homogeneous sorption of U(VI)	870:909	The isotherms could be described by the Langmuir model, suggesting mono-layer homogeneous sorption of U(VI).					
35872307	2	3	theme	composite	413:421	arg1	foam					429:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam	335:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal	335:501	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	1	4	theme	environmental	254:266	arg1	challenges					268:277	the environmental challenges	250:277	the environmental challenges of radioactive wastewater treatment	250:313	The development of new adsorbents is needed to address the environmental challenges of radioactive wastewater treatment.					
35872307	9	5	theme	PCM-40	1306:1311	arg1	capacity					1243:1250	The high sorption capacity	1225:1250	The high sorption capacity	1225:1250	The high sorption capacity, fast kinetic rate and relatively good selectivity of PCM-40 highlights its promising application in radioactive pollution cleanup.					
35872307	9	5	theme	PCM-40	1306:1311	arg1	selectivity					1291:1301	relatively good selectivity	1275:1301	relatively good selectivity	1275:1301	The high sorption capacity, fast kinetic rate and relatively good selectivity of PCM-40 highlights its promising application in radioactive pollution cleanup.					
35872307	9	5	theme	PCM-40	1306:1311	arg1	rate					1266:1269	fast kinetic rate	1253:1269	fast kinetic rate	1253:1269	The high sorption capacity, fast kinetic rate and relatively good selectivity of PCM-40 highlights its promising application in radioactive pollution cleanup.					
35872307	9	6	theme	high	1229:1232	arg1	capacity					1243:1250	The high sorption capacity	1225:1250	The high sorption capacity	1225:1250	The high sorption capacity, fast kinetic rate and relatively good selectivity of PCM-40 highlights its promising application in radioactive pollution cleanup.					
35872307	2	7	theme	honeycomb-like	398:411	arg1	foam					429:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam	335:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal	335:501	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	8	8	theme	ions	1178:1181	arg1	complexation					1155:1166	the complexation	1151:1166	the complexation of uranyl ions with the amine/imine and hydroxyl groups	1151:1222	The main sorption mechanism is related to the complexation of uranyl ions with the amine/imine and hydroxyl groups.					
35872307	6	9	theme	U	933:933	arg1	capacity					939:946	The maximum sorption U(VI) capacity	912:946	The maximum sorption U(VI) capacity for PCM-40	912:957	The maximum sorption U(VI) capacity for PCM-40 reaches up to 301.9 mg/g at pH 4.5 and 298 K.					
35872307	8	10	with	complexation	1155:1166	arg1	groups					1217:1222	the amine/imine and hydroxyl groups	1188:1222	groups	1217:1222	The main sorption mechanism is related to the complexation of uranyl ions with the amine/imine and hydroxyl groups.					
35872307	8	11	theme	uranyl	1171:1176	arg1	ions					1178:1181	uranyl ions	1171:1181	uranyl ions	1171:1181	The main sorption mechanism is related to the complexation of uranyl ions with the amine/imine and hydroxyl groups.					
35872307	6	12	theme	sorption	924:931	arg1	capacity					939:946	The maximum sorption U(VI) capacity	912:946	The maximum sorption U(VI) capacity for PCM-40	912:957	The maximum sorption U(VI) capacity for PCM-40 reaches up to 301.9 mg/g at pH 4.5 and 298 K.					
35872307	4	13	theme	pseudo-second-order	721:739	arg1	model					741:745	the pseudo-second-order model	717:745	the pseudo-second-order model	717:745	The kinetics were well-simulated by the pseudo-second-order model, indicating chemisorption as the rate-controlling step.					
35872307	1	14	theme	radioactive	282:292	arg1	treatment					305:313	radioactive wastewater treatment	282:313	radioactive wastewater treatment	282:313	The development of new adsorbents is needed to address the environmental challenges of radioactive wastewater treatment.					
35872307	0	15	theme	effective	145:153	arg1	removal					155:161	the effective removal	141:161	the effective removal of U(VI) from aqueous solution	141:192	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	6	16	theme	maximum	916:922	arg1	capacity					939:946	The maximum sorption U(VI) capacity	912:946	The maximum sorption U(VI) capacity for PCM-40	912:957	The maximum sorption U(VI) capacity for PCM-40 reaches up to 301.9 mg/g at pH 4.5 and 298 K.					
35872307	5	17	theme	homogeneous	881:891	arg1	sorption					893:900	mono-layer homogeneous sorption	870:900	mono-layer homogeneous sorption of U(VI)	870:909	The isotherms could be described by the Langmuir model, suggesting mono-layer homogeneous sorption of U(VI).					
35872307	2	18	theme	ultralight	465:474	arg1	property					476:483	ultralight property	465:483	ultralight property	465:483	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	3	19	theme	sorption	563:570	arg1	capacity					572:579	the highest sorption capacity	551:579	the highest sorption capacity for U	551:585	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	2	20	theme	remarkable	439:448	arg1	elasticity					450:459	remarkable elasticity	439:459	remarkable elasticity	439:459	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	9	21	theme	kinetic	1258:1264	arg1	rate					1266:1269	fast kinetic rate	1253:1269	fast kinetic rate	1253:1269	The high sorption capacity, fast kinetic rate and relatively good selectivity of PCM-40 highlights its promising application in radioactive pollution cleanup.					
35872307	5	22	theme	Langmuir	843:850	arg1	model					852:856	the Langmuir model	839:856	the Langmuir model	839:856	The isotherms could be described by the Langmuir model, suggesting mono-layer homogeneous sorption of U(VI).					
35872307	3	23	theme	highest	555:561	arg1	capacity					572:579	the highest sorption capacity	551:579	the highest sorption capacity for U	551:585	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	7	24	theme	process	1100:1106	arg1	nature					1075:1080	the spontaneous and endothermic nature	1043:1080	the spontaneous and endothermic nature of the adsorption process	1043:1106	The thermodynamic parameters revealed the spontaneous and endothermic nature of the adsorption process.					
35872307	9	25	theme	radioactive	1353:1363	arg1	cleanup					1375:1381	radioactive pollution cleanup	1353:1381	radioactive pollution cleanup	1353:1381	The high sorption capacity, fast kinetic rate and relatively good selectivity of PCM-40 highlights its promising application in radioactive pollution cleanup.					
35872307	2	26	theme	novel	337:341	arg1	foam					429:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam	335:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal	335:501	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	7	27	theme	endothermic	1063:1073	arg1	nature					1075:1080	the spontaneous and endothermic nature	1043:1080	the spontaneous and endothermic nature of the adsorption process	1043:1106	The thermodynamic parameters revealed the spontaneous and endothermic nature of the adsorption process.					
35872307	8	28	theme	amine/imine	1192:1202	arg1	groups					1217:1222	the amine/imine and hydroxyl groups	1188:1222	groups	1217:1222	The main sorption mechanism is related to the complexation of uranyl ions with the amine/imine and hydroxyl groups.					
35872307	1	29	theme	wastewater	294:303	arg1	treatment					305:313	radioactive wastewater treatment	282:313	radioactive wastewater treatment	282:313	The development of new adsorbents is needed to address the environmental challenges of radioactive wastewater treatment.					
35872307	9	30	theme	good	1286:1289	arg1	selectivity					1291:1301	relatively good selectivity	1275:1301	relatively good selectivity	1275:1301	The high sorption capacity, fast kinetic rate and relatively good selectivity of PCM-40 highlights its promising application in radioactive pollution cleanup.					
35872307	5	31	theme	U	905:905	arg1	sorption					893:900	mono-layer homogeneous sorption	870:900	mono-layer homogeneous sorption of U(VI)	870:909	The isotherms could be described by the Langmuir model, suggesting mono-layer homogeneous sorption of U(VI).					
35872307	6	32	dep	capacity	939:946	arg1	VI					935:936	VI	935:936	VI	935:936	The maximum sorption U(VI) capacity for PCM-40 reaches up to 301.9 mg/g at pH 4.5 and 298 K.					
35872307	0	33	theme	nanorod	47:53	arg1	foams					80:84	chitosan/α-MnO2 nanorod honeycomb-like composite foams	31:84	chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution	31:192	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	1	34	theme	treatment	305:313	arg1	challenges					268:277	the environmental challenges	250:277	the environmental challenges of radioactive wastewater treatment	250:313	The development of new adsorbents is needed to address the environmental challenges of radioactive wastewater treatment.					
35872307	9	35	theme	sorption	1234:1241	arg1	capacity					1243:1250	The high sorption capacity	1225:1250	The high sorption capacity	1225:1250	The high sorption capacity, fast kinetic rate and relatively good selectivity of PCM-40 highlights its promising application in radioactive pollution cleanup.					
35872307	3	36	dep	structure	631:639	arg1	VI					587:588	VI	587:588	VI	587:588	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	0	37	theme	chitosan/α-MnO2	31:45	arg1	foams					80:84	chitosan/α-MnO2 nanorod honeycomb-like composite foams	31:84	chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution	31:192	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	7	38	theme	adsorption	1089:1098	arg1	process					1100:1106	the adsorption process	1085:1106	the adsorption process	1085:1106	The thermodynamic parameters revealed the spontaneous and endothermic nature of the adsorption process.					
35872307	3	39	theme	amine/imine	661:671	arg1	groups					673:678	amine/imine groups	661:678	amine/imine groups	661:678	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	4	40	theme	rate-controlling	780:795	arg1	step					797:800	the rate-controlling step	776:800	the rate-controlling step	776:800	The kinetics were well-simulated by the pseudo-second-order model, indicating chemisorption as the rate-controlling step.					
35872307	4	40	theme	rate-controlling	780:795	arg1	chemisorption					759:771	chemisorption	759:771	chemisorption	759:771	The kinetics were well-simulated by the pseudo-second-order model, indicating chemisorption as the rate-controlling step.					
35872307	1	41	theme	new	214:216	arg1	adsorbents					218:227	new adsorbents	214:227	new adsorbents	214:227	The development of new adsorbents is needed to address the environmental challenges of radioactive wastewater treatment.					
35872307	0	42	theme	composite	70:78	arg1	foams					80:84	chitosan/α-MnO2 nanorod honeycomb-like composite foams	31:84	chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution	31:192	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	0	43	from	solution	185:192	arg1	removal					155:161	the effective removal	141:161	the effective removal of U(VI) from aqueous solution	141:192	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	8	44	theme	main	1113:1116	arg1	mechanism					1127:1135	The main sorption mechanism	1109:1135	The main sorption mechanism	1109:1135	The main sorption mechanism is related to the complexation of uranyl ions with the amine/imine and hydroxyl groups.					
35872307	8	44	theme	main	1113:1116	arg1	related					1140:1146	related	1140:1146	related	1140:1146	The main sorption mechanism is related to the complexation of uranyl ions with the amine/imine and hydroxyl groups.					
35872307	3	45	theme	groups	673:678	arg1	structure					631:639	its highly developed macroporous structure	598:639	its highly developed macroporous structure	598:639	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	3	45	theme	groups	673:678	arg1	content					650:656	high content	645:656	high content of amine/imine groups	645:678	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	1	46	theme	adsorbents	218:227	arg1	development					199:209	The development	195:209	The development of new adsorbents	195:227	The development of new adsorbents is needed to address the environmental challenges of radioactive wastewater treatment.					
35872307	0	47	theme	honeycomb-like	55:68	arg1	foams					80:84	chitosan/α-MnO2 nanorod honeycomb-like composite foams	31:84	chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution	31:192	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	0	48	theme	U	166:166	arg1	removal					155:161	the effective removal	141:161	the effective removal of U(VI) from aqueous solution	141:192	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	9	49	theme	fast	1253:1256	arg1	rate					1266:1269	fast kinetic rate	1253:1269	fast kinetic rate	1253:1269	The high sorption capacity, fast kinetic rate and relatively good selectivity of PCM-40 highlights its promising application in radioactive pollution cleanup.					
35872307	2	50	theme	U	489:489	arg1	removal					495:501	U(VI) removal	489:501	U(VI) removal	489:501	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	9	51	theme	pollution	1365:1373	arg1	cleanup					1375:1381	radioactive pollution cleanup	1353:1381	radioactive pollution cleanup	1353:1381	The high sorption capacity, fast kinetic rate and relatively good selectivity of PCM-40 highlights its promising application in radioactive pollution cleanup.					
35872307	5	52	dep	U	905:905	arg1	VI					907:908	VI	907:908	VI	907:908	The isotherms could be described by the Langmuir model, suggesting mono-layer homogeneous sorption of U(VI).					
35872307	8	53	theme	sorption	1118:1125	arg1	mechanism					1127:1135	The main sorption mechanism	1109:1135	The main sorption mechanism	1109:1135	The main sorption mechanism is related to the complexation of uranyl ions with the amine/imine and hydroxyl groups.					
35872307	8	53	theme	sorption	1118:1125	arg1	related					1140:1146	related	1140:1146	related	1140:1146	The main sorption mechanism is related to the complexation of uranyl ions with the amine/imine and hydroxyl groups.					
35872307	2	54	theme	nanorod	390:396	arg1	foam					429:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam	335:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal	335:501	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	3	55	contain	possessed	541:549	arg2	capacity					572:579	the highest sorption capacity	551:579	the highest sorption capacity for U	551:585	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	3	55	contain	possessed	541:549	arg1	PCM-40					534:539	PCM-40	534:539	PCM-40	534:539	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	7	56	theme	thermodynamic	1009:1021	arg1	parameters					1023:1032	The thermodynamic parameters	1005:1032	The thermodynamic parameters	1005:1032	The thermodynamic parameters revealed the spontaneous and endothermic nature of the adsorption process.					
35872307	2	57	theme	chitosan/α-MnO2	374:388	arg1	foam					429:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam	335:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal	335:501	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	0	58	theme	aqueous	177:183	arg1	solution					185:192	aqueous solution	177:192	aqueous solution	177:192	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	3	59	theme	developed	609:617	arg1	structure					631:639	its highly developed macroporous structure	598:639	its highly developed macroporous structure	598:639	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	2	60	theme	incorporated	361:372	arg1	foam					429:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam	335:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal	335:501	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	7	61	theme	spontaneous	1047:1057	arg1	nature					1075:1080	the spontaneous and endothermic nature	1043:1080	the spontaneous and endothermic nature of the adsorption process	1043:1106	The thermodynamic parameters revealed the spontaneous and endothermic nature of the adsorption process.					
35872307	3	62	theme	high	645:648	arg1	content					650:656	high content	645:656	high content of amine/imine groups	645:678	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	2	63	theme	polyethyleneimine	343:359	arg1	foam					429:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam	335:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal	335:501	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	3	64	theme	different	510:518	arg1	sorbents					524:531	different PCM sorbents	510:531	different PCM sorbents	510:531	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	6	65	dep	301.9 mg/g	973:982	arg1	up					967:968	up	967:968	up	967:968	The maximum sorption U(VI) capacity for PCM-40 reaches up to 301.9 mg/g at pH 4.5 and 298 K.					
35872307	3	66	theme	macroporous	619:629	arg1	structure					631:639	its highly developed macroporous structure	598:639	its highly developed macroporous structure	598:639	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	0	67	with	foams	80:84	arg1	elasticity					102:111	remarkable elasticity	91:111	remarkable elasticity	91:111	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	0	67	with	foams	80:84	arg1	property					128:135	ultralight property	117:135	ultralight property	117:135	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	2	68	theme	PCM	424:426	arg1	foam					429:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam	335:432	a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal	335:501	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	3	69	theme	PCM	520:522	arg1	sorbents					524:531	different PCM sorbents	510:531	different PCM sorbents	510:531	Among different PCM sorbents, PCM-40 possessed the highest sorption capacity for U(VI) due to its highly developed macroporous structure and high content of amine/imine groups.					
35872307	9	70	theme	promising	1328:1336	arg1	application					1338:1348	its promising application	1324:1348	its promising application in radioactive pollution cleanup	1324:1381	The high sorption capacity, fast kinetic rate and relatively good selectivity of PCM-40 highlights its promising application in radioactive pollution cleanup.					
35872307	0	71	theme	remarkable	91:100	arg1	elasticity					102:111	remarkable elasticity	91:111	remarkable elasticity	91:111	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	2	72	with	foam	429:432	arg1	elasticity					450:459	remarkable elasticity	439:459	remarkable elasticity	439:459	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	2	72	with	foam	429:432	arg1	property					476:483	ultralight property	465:483	ultralight property	465:483	Herein we reported a novel polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite (PCM) foam with remarkable elasticity and ultralight property for U(VI) removal.					
35872307	8	73	theme	hydroxyl	1208:1215	arg1	groups					1217:1222	the amine/imine and hydroxyl groups	1188:1222	groups	1217:1222	The main sorption mechanism is related to the complexation of uranyl ions with the amine/imine and hydroxyl groups.					
35872307	0	74	dep	U	166:166	arg1	VI					168:169	VI	168:169	VI	168:169	Polyethyleneimine incorporated chitosan/α-MnO2 nanorod honeycomb-like composite foams with remarkable elasticity and ultralight property for the effective removal of U(VI) from aqueous solution.					
35872307	9	75	from	application	1338:1348	arg1	cleanup					1375:1381	radioactive pollution cleanup	1353:1381	radioactive pollution cleanup	1353:1381	The high sorption capacity, fast kinetic rate and relatively good selectivity of PCM-40 highlights its promising application in radioactive pollution cleanup.					
36889414	0	0	contain	containing	73:82	arg1	particles					63:71	lyophilized particles	51:71	lyophilized particles containing probiotics and prebiotics	51:108	Improved infectious burn wound healing by applying lyophilized particles containing probiotics and prebiotics.					
36889414	0	0	contain	containing	73:82	arg2	prebiotics					99:108	prebiotics	99:108	prebiotics	99:108	Improved infectious burn wound healing by applying lyophilized particles containing probiotics and prebiotics.					
36889414	0	0	contain	containing	73:82	arg2	probiotics					84:93	probiotics	84:93	probiotics	84:93	Improved infectious burn wound healing by applying lyophilized particles containing probiotics and prebiotics.					
36889414	2	1	theme	prebiotics	378:387	arg1	effects					316:322	the effects	312:322	the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations	312:456	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	2	2	from	effects	316:322	arg1	viability					406:414	probiotic viability	396:414	probiotic viability	396:414	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	2	2	from	effects	316:322	arg1	behavior					429:436	swelling behavior	420:436	swelling behavior	420:436	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	5	3	contain	had	700:702	arg1	formula					692:698	The optimized formula	678:698	The optimized formula	678:698	The optimized formula had a viability percentage of more than 82%, with the stability studies suggesting that the powders should be stored at refrigerated temperatures.					
36889414	5	3	contain	had	700:702	arg2	percentage					716:725	a viability percentage	704:725	a viability percentage of more than 82%	704:742	The optimized formula had a viability percentage of more than 82%, with the stability studies suggesting that the powders should be stored at refrigerated temperatures.					
36889414	5	4	theme	viability	706:714	arg1	percentage					716:725	a viability percentage	704:725	a viability percentage of more than 82%	704:742	The optimized formula had a viability percentage of more than 82%, with the stability studies suggesting that the powders should be stored at refrigerated temperatures.					
36889414	9	5	theme	wound	1240:1244	arg1	closure					1246:1252	wound closure	1240:1252	wound closure	1240:1252	The optimized formula resulted in a higher rate of wound closure and infection clearance.					
36889414	1	6	theme	cationic	181:188	arg1	polymers					202:209	cationic and anionic polymers	181:209	cationic and anionic polymers	181:209	Lactiplantibacillus plantarum cells were encapsulated in a mixture of cationic and anionic polymers, with the final composition stabilized through freeze-drying.					
36889414	2	7	theme	adding	371:376	arg1	prebiotics					378:387	adding prebiotics	371:387	different polymer concentrations as well as adding prebiotics	327:387	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	7	8	theme	pathogen	1019:1026	arg1	inhibition					1028:1037	pathogen inhibition	1019:1037	pathogen inhibition	1019:1037	According to antimicrobial evaluations, the difference in pathogen inhibition between formulated and fresh probiotics was less than a logarithm.					
36889414	7	9	theme	fresh	1062:1066	arg1	probiotics					1068:1077	fresh probiotics	1062:1077	fresh probiotics	1062:1077	According to antimicrobial evaluations, the difference in pathogen inhibition between formulated and fresh probiotics was less than a logarithm.					
36889414	8	10	theme	final	1110:1114	arg1	formula					1116:1122	The final formula	1106:1122	The final formula	1106:1122	The final formula was tested in vivo and showed improved wound healing indicators.					
36889414	6	11	theme	physical	851:858	arg1	characteristics					860:874	The physical characteristics	847:874	The physical characteristics of the optimized formula	847:899	The physical characteristics of the optimized formula were examined to ensure compatibility with its application.					
36889414	11	12	theme	histological	1408:1419	arg1	investigations					1421:1434	histological investigations	1408:1434	histological investigations	1408:1434	In histological investigations, the probiotic-loaded particles functioned exactly as efficaciously as silver sulfadiazine ointment did.					
36889414	11	13	theme	probiotic-loaded	1441:1456	arg1	particles					1458:1466	the probiotic-loaded particles	1437:1466	the probiotic-loaded particles	1437:1466	In histological investigations, the probiotic-loaded particles functioned exactly as efficaciously as silver sulfadiazine ointment did.					
36889414	4	14	theme	%	647:647	arg1	percentages					621:631	initial swelling percentages	604:631	initial swelling percentages of around 2000% for the optimal formulation	604:675	These images corresponded to initial swelling percentages of around 2000% for the optimal formulation.					
36889414	11	15	theme	silver	1507:1512	arg1	ointment					1527:1534	silver sulfadiazine ointment	1507:1534	silver sulfadiazine ointment did	1507:1538	In histological investigations, the probiotic-loaded particles functioned exactly as efficaciously as silver sulfadiazine ointment did.					
36889414	9	16	theme	infection	1258:1266	arg1	clearance					1268:1276	infection clearance	1258:1276	infection clearance	1258:1276	The optimized formula resulted in a higher rate of wound closure and infection clearance.					
36889414	6	17	with	compatibility	925:937	arg1	application					948:958	its application	944:958	its application	944:958	The physical characteristics of the optimized formula were examined to ensure compatibility with its application.					
36889414	2	18	theme	swelling	420:427	arg1	behavior					429:436	swelling behavior	420:436	swelling behavior	420:436	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	11	19	theme	sulfadiazine	1514:1525	arg1	ointment					1527:1534	silver sulfadiazine ointment	1507:1534	silver sulfadiazine ointment did	1507:1538	In histological investigations, the probiotic-loaded particles functioned exactly as efficaciously as silver sulfadiazine ointment did.					
36889414	5	20	theme	optimized	682:690	arg1	formula					692:698	The optimized formula	678:698	The optimized formula	678:698	The optimized formula had a viability percentage of more than 82%, with the stability studies suggesting that the powders should be stored at refrigerated temperatures.					
36889414	9	21	theme	clearance	1268:1276	arg1	rate					1232:1235	a higher rate	1223:1235	a higher rate of wound closure and infection clearance	1223:1276	The optimized formula resulted in a higher rate of wound closure and infection clearance.					
36889414	1	22	theme	anionic	194:200	arg1	polymers					202:209	cationic and anionic polymers	181:209	cationic and anionic polymers	181:209	Lactiplantibacillus plantarum cells were encapsulated in a mixture of cationic and anionic polymers, with the final composition stabilized through freeze-drying.					
36889414	0	23	theme	infectious	9:18	arg1	healing					31:37	infectious burn wound healing	9:37	infectious burn wound healing	9:37	Improved infectious burn wound healing by applying lyophilized particles containing probiotics and prebiotics.					
36889414	2	24	theme	polymer	337:343	arg1	concentrations					345:358	different polymer concentrations	327:358	different polymer concentrations as well as adding prebiotics	327:387	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	1	25	theme	polymers	202:209	arg1	mixture					170:176	a mixture	168:176	a mixture of cationic and anionic polymers	168:209	Lactiplantibacillus plantarum cells were encapsulated in a mixture of cationic and anionic polymers, with the final composition stabilized through freeze-drying.					
36889414	4	26	theme	swelling	612:619	arg1	percentages					621:631	initial swelling percentages	604:631	initial swelling percentages of around 2000% for the optimal formulation	604:675	These images corresponded to initial swelling percentages of around 2000% for the optimal formulation.					
36889414	2	27	theme	different	327:335	arg1	concentrations					345:358	different polymer concentrations	327:358	different polymer concentrations as well as adding prebiotics	327:387	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	2	28	theme	probiotic	396:404	arg1	viability					406:414	probiotic viability	396:414	probiotic viability	396:414	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	0	29	theme	wound	25:29	arg1	healing					31:37	infectious burn wound healing	9:37	infectious burn wound healing	9:37	Improved infectious burn wound healing by applying lyophilized particles containing probiotics and prebiotics.					
36889414	4	30	theme	initial	604:610	arg1	percentages					621:631	initial swelling percentages	604:631	initial swelling percentages of around 2000% for the optimal formulation	604:675	These images corresponded to initial swelling percentages of around 2000% for the optimal formulation.					
36889414	1	31	theme	Lactiplantibacillus	111:129	arg1	cells					141:145	Lactiplantibacillus plantarum cells	111:145	Lactiplantibacillus plantarum cells	111:145	Lactiplantibacillus plantarum cells were encapsulated in a mixture of cationic and anionic polymers, with the final composition stabilized through freeze-drying.					
36889414	2	32	theme	formulations	445:456	arg1	viability					406:414	probiotic viability	396:414	probiotic viability	396:414	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	2	32	theme	formulations	445:456	arg1	behavior					429:436	swelling behavior	420:436	swelling behavior	420:436	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	0	33	theme	burn	20:23	arg1	healing					31:37	infectious burn wound healing	9:37	infectious burn wound healing	9:37	Improved infectious burn wound healing by applying lyophilized particles containing probiotics and prebiotics.					
36889414	1	34	theme	plantarum	131:139	arg1	cells					141:145	Lactiplantibacillus plantarum cells	111:145	Lactiplantibacillus plantarum cells	111:145	Lactiplantibacillus plantarum cells were encapsulated in a mixture of cationic and anionic polymers, with the final composition stabilized through freeze-drying.					
36889414	10	35	theme	inflammatory	1381:1392	arg1	responses					1394:1402	wound inflammatory responses	1375:1402	wound inflammatory responses	1375:1402	Furthermore, the molecular studies for oxidative stress indicated that the formula could modify wound inflammatory responses.					
36889414	3	36	theme	capable	516:522	arg1	particles					506:514	stacked particles	498:514	stacked particles capable of rapidly absorbing significant amounts of water	498:572	Scanning electron micrographs revealed stacked particles capable of rapidly absorbing significant amounts of water.					
36889414	8	37	theme	wound	1163:1167	arg1	healing					1169:1175	improved wound healing	1154:1175	improved wound healing indicators	1154:1186	The final formula was tested in vivo and showed improved wound healing indicators.					
36889414	3	38	theme	Scanning	459:466	arg1	micrographs					477:487	Scanning electron micrographs	459:487	Scanning electron micrographs	459:487	Scanning electron micrographs revealed stacked particles capable of rapidly absorbing significant amounts of water.					
36889414	1	39	theme	final	221:225	arg1	composition					227:237	the final composition	217:237	the final composition stabilized through freeze-drying	217:270	Lactiplantibacillus plantarum cells were encapsulated in a mixture of cationic and anionic polymers, with the final composition stabilized through freeze-drying.					
36889414	3	40	theme	electron	468:475	arg1	micrographs					477:487	Scanning electron micrographs	459:487	Scanning electron micrographs	459:487	Scanning electron micrographs revealed stacked particles capable of rapidly absorbing significant amounts of water.					
36889414	5	41	theme	stability	754:762	arg1	studies					764:770	the stability studies	750:770	the stability studies suggesting that the powders should be stored at refrigerated temperatures	750:844	The optimized formula had a viability percentage of more than 82%, with the stability studies suggesting that the powders should be stored at refrigerated temperatures.					
36889414	9	42	theme	optimized	1193:1201	arg1	formula					1203:1209	The optimized formula	1189:1209	The optimized formula	1189:1209	The optimized formula resulted in a higher rate of wound closure and infection clearance.					
36889414	3	43	theme	stacked	498:504	arg1	particles					506:514	stacked particles	498:514	stacked particles capable of rapidly absorbing significant amounts of water	498:572	Scanning electron micrographs revealed stacked particles capable of rapidly absorbing significant amounts of water.					
36889414	0	44	theme	lyophilized	51:61	arg1	particles					63:71	lyophilized particles	51:71	lyophilized particles containing probiotics and prebiotics	51:108	Improved infectious burn wound healing by applying lyophilized particles containing probiotics and prebiotics.					
36889414	10	45	theme	wound	1375:1379	arg1	responses					1394:1402	wound inflammatory responses	1375:1402	wound inflammatory responses	1375:1402	Furthermore, the molecular studies for oxidative stress indicated that the formula could modify wound inflammatory responses.					
36889414	8	46	theme	improved	1154:1161	arg1	healing					1169:1175	improved wound healing	1154:1175	improved wound healing indicators	1154:1186	The final formula was tested in vivo and showed improved wound healing indicators.					
36889414	8	47	theme	healing	1169:1175	arg1	indicators					1177:1186	improved wound healing indicators	1154:1186	improved wound healing indicators	1154:1186	The final formula was tested in vivo and showed improved wound healing indicators.					
36889414	3	48	theme	significant	545:555	arg1	water					568:572	water	568:572	water	568:572	Scanning electron micrographs revealed stacked particles capable of rapidly absorbing significant amounts of water.					
36889414	3	48	theme	significant	545:555	arg1	amounts					557:563	significant amounts	545:563	significant amounts of water	545:572	Scanning electron micrographs revealed stacked particles capable of rapidly absorbing significant amounts of water.					
36889414	7	49	theme	antimicrobial	974:986	arg1	evaluations					988:998	antimicrobial evaluations	974:998	antimicrobial evaluations	974:998	According to antimicrobial evaluations, the difference in pathogen inhibition between formulated and fresh probiotics was less than a logarithm.					
36889414	6	50	theme	formula	893:899	arg1	characteristics					860:874	The physical characteristics	847:874	The physical characteristics of the optimized formula	847:899	The physical characteristics of the optimized formula were examined to ensure compatibility with its application.					
36889414	2	51	dep	viability	406:414	arg1	the					392:394	the	392:394	the	392:394	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	9	52	theme	closure	1246:1252	arg1	rate					1232:1235	a higher rate	1223:1235	a higher rate of wound closure and infection clearance	1223:1276	The optimized formula resulted in a higher rate of wound closure and infection clearance.					
36889414	7	53	from	difference	1005:1014	arg1	inhibition					1028:1037	pathogen inhibition	1019:1037	pathogen inhibition	1019:1037	According to antimicrobial evaluations, the difference in pathogen inhibition between formulated and fresh probiotics was less than a logarithm.					
36889414	4	54	theme	optimal	657:663	arg1	formulation					665:675	the optimal formulation	653:675	the optimal formulation	653:675	These images corresponded to initial swelling percentages of around 2000% for the optimal formulation.					
36889414	5	55	theme	%	742:742	arg1	percentage					716:725	a viability percentage	704:725	a viability percentage of more than 82%	704:742	The optimized formula had a viability percentage of more than 82%, with the stability studies suggesting that the powders should be stored at refrigerated temperatures.					
36889414	6	56	theme	optimized	883:891	arg1	formula					893:899	the optimized formula	879:899	the optimized formula	879:899	The physical characteristics of the optimized formula were examined to ensure compatibility with its application.					
36889414	2	57	theme	concentrations	345:358	arg1	effects					316:322	the effects	312:322	the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations	312:456	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	10	58	theme	molecular	1296:1304	arg1	studies					1306:1312	the molecular studies	1292:1312	the molecular studies for oxidative stress	1292:1333	Furthermore, the molecular studies for oxidative stress indicated that the formula could modify wound inflammatory responses.					
36889414	10	59	theme	oxidative	1318:1326	arg1	stress					1328:1333	oxidative stress	1318:1333	oxidative stress	1318:1333	Furthermore, the molecular studies for oxidative stress indicated that the formula could modify wound inflammatory responses.					
36889414	2	60	theme	D-optimal	275:283	arg1	design					285:290	A D-optimal design	273:290	A D-optimal design	273:290	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36889414	9	61	theme	higher	1225:1230	arg1	rate					1232:1235	a higher rate	1223:1235	a higher rate of wound closure and infection clearance	1223:1276	The optimized formula resulted in a higher rate of wound closure and infection clearance.					
36889414	3	62	theme	water	568:572	arg1	water					568:572	water	568:572	water	568:572	Scanning electron micrographs revealed stacked particles capable of rapidly absorbing significant amounts of water.					
36889414	3	62	theme	water	568:572	arg1	amounts					557:563	significant amounts	545:563	significant amounts of water	545:572	Scanning electron micrographs revealed stacked particles capable of rapidly absorbing significant amounts of water.					
36889414	5	63	theme	refrigerated	820:831	arg1	temperatures					833:844	refrigerated temperatures	820:844	refrigerated temperatures	820:844	The optimized formula had a viability percentage of more than 82%, with the stability studies suggesting that the powders should be stored at refrigerated temperatures.					
36889414	2	64	used	used	296:299	arg2	design					285:290	A D-optimal design	273:290	A D-optimal design	273:290	A D-optimal design was used to examine the effects of different polymer concentrations as well as adding prebiotics on the probiotic viability and swelling behavior of the formulations.					
36478231	6	0	theme	southern	885:892	arg1	China					894:898	southern China	885:898	southern China	885:898	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	10	1	from	variations	1545:1554	arg1	strength					1564:1571	stem strength	1559:1571	stem strength	1559:1571	Taken together, our results show that DNA methylation in lignin-related genes regulates variations in stem strength and lodging in weedy rice, and between weed strains and co-occurring cultivated rice.					
36478231	6	2	theme	lodging	776:782	arg1	degree					766:771	the degree	762:771	the degree of lodging	762:782	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	6	3	link	indica-derived	799:812	arg1	strains					819:825	indica-derived weed strains	799:825	indica-derived weed strains with high lodging levels, which occurred predominantly in southern China,	799:899	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	4	4	theme	cultivated	548:557	arg1	rice					559:562	co-occurring cultivated rice	535:562	co-occurring cultivated rice	535:562	Here, we compared lodging variation among diverse Chinese weedy rice strains and between weedy rice and co-occurring cultivated rice.					
36478231	2	5	theme	cultivated	113:122	arg1	rice					124:127	cultivated rice	113:127	cultivated rice	113:127	, one of the most notorious weeds of cultivated rice, evades eradication through stem lodging and seed shattering.					
36478231	9	6	theme	expression	1384:1393	arg1	levels					1395:1400	their expression levels	1378:1400	their expression levels	1378:1400	Variation in DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3 mediated the differences in their expression levels and affected the ratio of cellulose to lignin content.					
36478231	8	7	theme	co-occurring	1139:1150	arg1	rice					1163:1166	co-occurring cultivated rice	1139:1166	co-occurring cultivated rice	1139:1166	In comparison to co-occurring cultivated rice, there was a lower ratio of cellulose to lignin content in the lodging-prone weedy rice.					
36478231	8	8	theme	cellulose	1196:1204	arg1	ratio					1187:1191	a lower ratio	1179:1191	a lower ratio of cellulose to lignin content in the lodging-prone weedy rice	1179:1254	In comparison to co-occurring cultivated rice, there was a lower ratio of cellulose to lignin content in the lodging-prone weedy rice.					
36478231	2	9	theme	weeds	104:108	arg1	weeds					104:108	the most notorious weeds	85:108	the most notorious weeds of cultivated rice	85:127	, one of the most notorious weeds of cultivated rice, evades eradication through stem lodging and seed shattering.					
36478231	2	9	theme	weeds	104:108	arg1	one					78:80	one	78:80	one	78:80	, one of the most notorious weeds of cultivated rice, evades eradication through stem lodging and seed shattering.					
36478231	8	10	theme	lignin	1209:1214	arg1	content					1216:1222	lignin content	1209:1222	lignin content in the lodging-prone weedy rice	1209:1254	In comparison to co-occurring cultivated rice, there was a lower ratio of cellulose to lignin content in the lodging-prone weedy rice.					
36478231	10	11	theme	stem	1559:1562	arg1	strength					1564:1571	stem strength	1559:1571	stem strength	1559:1571	Taken together, our results show that DNA methylation in lignin-related genes regulates variations in stem strength and lodging in weedy rice, and between weed strains and co-occurring cultivated rice.					
36478231	5	12	theme	stems	599:603	arg1	composition					578:588	The chemical composition	565:588	The chemical composition of basal stems	565:603	The chemical composition of basal stems was determined, and transcriptome and methylome sequencing were used to assess the variation in expression of lodging-related genes.					
36478231	6	13	theme	lodging	941:947	arg1	levels					949:954	lower lodging levels	935:954	lower lodging levels	935:954	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	6	14	with	strains	922:928	arg1	levels					845:850	high lodging levels	832:850	high lodging levels	832:850	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	6	14	with	strains	922:928	arg1	levels					949:954	lower lodging levels	935:954	lower lodging levels	935:954	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	7	15	dep	content	1085:1091	arg1	lower					1072:1076	lower	1072:1076	lower	1072:1076	The more lodging-prone indica weedy rice had a smaller bending stress and lower lignin content than non-lodging accessions.					
36478231	3	16	theme	seed	220:223	arg1	shattering					225:234	seed shattering	220:234	seed shattering	220:234	Many studies have focused on seed shattering, whereas variations in lodging have received less attention and the underlying mechanisms that cause the differences in lodging between weedy and cultivated rice have not been studied in detail.					
36478231	8	17	theme	lodging-prone	1231:1243	arg1	rice					1251:1254	the lodging-prone weedy rice	1227:1254	the lodging-prone weedy rice	1227:1254	In comparison to co-occurring cultivated rice, there was a lower ratio of cellulose to lignin content in the lodging-prone weedy rice.					
36478231	7	18	theme	weedy	1028:1032	arg1	rice					1034:1037	The more lodging-prone indica weedy rice	998:1037	The more lodging-prone indica weedy rice	998:1037	The more lodging-prone indica weedy rice had a smaller bending stress and lower lignin content than non-lodging accessions.					
36478231	5	19	used	used	669:672	arg2	transcriptome					625:637	transcriptome	625:637	transcriptome	625:637	The chemical composition of basal stems was determined, and transcriptome and methylome sequencing were used to assess the variation in expression of lodging-related genes.					
36478231	5	19	used	used	669:672	arg2	sequencing					653:662	methylome sequencing	643:662	methylome sequencing	643:662	The chemical composition of basal stems was determined, and transcriptome and methylome sequencing were used to assess the variation in expression of lodging-related genes.					
36478231	6	20	theme	japonica-derived	905:920	arg1	strains					922:928	japonica-derived strains	905:928	japonica-derived strains with lower lodging levels, which were found primarily in the north	905:995	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	7	21	theme	lodging-prone	1007:1019	arg1	rice					1034:1037	The more lodging-prone indica weedy rice	998:1037	The more lodging-prone indica weedy rice	998:1037	The more lodging-prone indica weedy rice had a smaller bending stress and lower lignin content than non-lodging accessions.					
36478231	2	22	theme	seed	174:177	arg1	shattering					179:188	seed shattering	174:188	seed shattering	174:188	, one of the most notorious weeds of cultivated rice, evades eradication through stem lodging and seed shattering.					
36478231	5	23	theme	lodging-related	715:729	arg1	genes					731:735	lodging-related genes	715:735	lodging-related genes	715:735	The chemical composition of basal stems was determined, and transcriptome and methylome sequencing were used to assess the variation in expression of lodging-related genes.					
36478231	6	24	link	japonica-derived	905:920	arg1	strains					922:928	japonica-derived strains	905:928	japonica-derived strains with lower lodging levels, which were found primarily in the north	905:995	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	3	25	from	differences	341:351	arg1	lodging					356:362	lodging	356:362	lodging	356:362	Many studies have focused on seed shattering, whereas variations in lodging have received less attention and the underlying mechanisms that cause the differences in lodging between weedy and cultivated rice have not been studied in detail.					
36478231	6	26	with	strains	819:825	arg1	levels					845:850	high lodging levels	832:850	high lodging levels	832:850	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	6	26	with	strains	819:825	arg1	levels					949:954	lower lodging levels	935:954	lower lodging levels	935:954	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	3	27	theme	cultivated	382:391	arg1	rice					393:396	cultivated rice	382:396	cultivated rice	382:396	Many studies have focused on seed shattering, whereas variations in lodging have received less attention and the underlying mechanisms that cause the differences in lodging between weedy and cultivated rice have not been studied in detail.					
36478231	9	28	theme	lignin	1289:1294	arg1	OsSWN1					1314:1319	lignin synthesis-related OsSWN1	1289:1319	lignin synthesis-related OsSWN1	1289:1319	Variation in DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3 mediated the differences in their expression levels and affected the ratio of cellulose to lignin content.					
36478231	10	29	theme	co-occurring	1629:1640	arg1	rice					1653:1656	co-occurring cultivated rice	1629:1656	co-occurring cultivated rice	1629:1656	Taken together, our results show that DNA methylation in lignin-related genes regulates variations in stem strength and lodging in weedy rice, and between weed strains and co-occurring cultivated rice.					
36478231	0	30	theme	lodging	20:26	arg1	variation					28:36	lodging variation	20:36	lodging variation of weedy rice	20:50	Characterization of lodging variation of weedy rice.					
36478231	9	31	theme	OsSWN1	1314:1319	arg1	methylation					1274:1284	DNA methylation	1270:1284	DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3	1270:1348	Variation in DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3 mediated the differences in their expression levels and affected the ratio of cellulose to lignin content.					
36478231	6	32	theme	lodging	837:843	arg1	levels					845:850	high lodging levels	832:850	high lodging levels	832:850	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	9	33	theme	OsMYBX9	1322:1328	arg1	methylation					1274:1284	DNA methylation	1270:1284	DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3	1270:1348	Variation in DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3 mediated the differences in their expression levels and affected the ratio of cellulose to lignin content.					
36478231	10	34	from	methylation	1499:1509	arg1	genes					1529:1533	lignin-related genes	1514:1533	lignin-related genes	1514:1533	Taken together, our results show that DNA methylation in lignin-related genes regulates variations in stem strength and lodging in weedy rice, and between weed strains and co-occurring cultivated rice.					
36478231	4	35	theme	diverse	473:479	arg1	strains					500:506	diverse Chinese weedy rice strains	473:506	diverse Chinese weedy rice strains	473:506	Here, we compared lodging variation among diverse Chinese weedy rice strains and between weedy rice and co-occurring cultivated rice.					
36478231	2	36	theme	rice	124:127	arg1	weeds					104:108	the most notorious weeds	85:108	the most notorious weeds of cultivated rice	85:127	, one of the most notorious weeds of cultivated rice, evades eradication through stem lodging and seed shattering.					
36478231	0	37	theme	rice	47:50	arg1	variation					28:36	lodging variation	20:36	lodging variation of weedy rice	20:50	Characterization of lodging variation of weedy rice.					
36478231	9	38	theme	OsPAL1	1331:1336	arg1	methylation					1274:1284	DNA methylation	1270:1284	DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3	1270:1348	Variation in DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3 mediated the differences in their expression levels and affected the ratio of cellulose to lignin content.					
36478231	6	39	theme	weed	814:817	arg1	strains					819:825	indica-derived weed strains	799:825	indica-derived weed strains with high lodging levels, which occurred predominantly in southern China,	799:899	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	4	40	theme	weedy	489:493	arg1	strains					500:506	diverse Chinese weedy rice strains	473:506	diverse Chinese weedy rice strains	473:506	Here, we compared lodging variation among diverse Chinese weedy rice strains and between weedy rice and co-occurring cultivated rice.					
36478231	8	41	dep	rice	1163:1166	arg1	comparison					1125:1134	comparison	1125:1134	comparison	1125:1134	In comparison to co-occurring cultivated rice, there was a lower ratio of cellulose to lignin content in the lodging-prone weedy rice.					
36478231	3	42	from	variations	245:254	arg1	lodging					259:265	lodging	259:265	lodging	259:265	Many studies have focused on seed shattering, whereas variations in lodging have received less attention and the underlying mechanisms that cause the differences in lodging between weedy and cultivated rice have not been studied in detail.					
36478231	7	43	theme	lignin	1078:1083	arg1	content					1085:1091	lignin content	1078:1091	lignin content than non-lodging accessions	1078:1119	The more lodging-prone indica weedy rice had a smaller bending stress and lower lignin content than non-lodging accessions.					
36478231	10	44	from	strength	1564:1571	arg1	rice					1594:1597	weedy rice	1588:1597	weedy rice	1588:1597	Taken together, our results show that DNA methylation in lignin-related genes regulates variations in stem strength and lodging in weedy rice, and between weed strains and co-occurring cultivated rice.					
36478231	3	45	theme	Many	191:194	arg1	studies					196:202	Many studies	191:202	Many studies	191:202	Many studies have focused on seed shattering, whereas variations in lodging have received less attention and the underlying mechanisms that cause the differences in lodging between weedy and cultivated rice have not been studied in detail.					
36478231	4	46	theme	co-occurring	535:546	arg1	rice					559:562	co-occurring cultivated rice	535:562	co-occurring cultivated rice	535:562	Here, we compared lodging variation among diverse Chinese weedy rice strains and between weedy rice and co-occurring cultivated rice.					
36478231	10	47	theme	lignin-related	1514:1527	arg1	genes					1529:1533	lignin-related genes	1514:1533	lignin-related genes	1514:1533	Taken together, our results show that DNA methylation in lignin-related genes regulates variations in stem strength and lodging in weedy rice, and between weed strains and co-occurring cultivated rice.					
36478231	7	48	theme	bending	1053:1059	arg1	stress					1061:1066	a smaller bending stress	1043:1066	a smaller bending stress	1043:1066	The more lodging-prone indica weedy rice had a smaller bending stress and lower lignin content than non-lodging accessions.					
36478231	7	49	theme	non-lodging	1098:1108	arg1	accessions					1110:1119	non-lodging accessions	1098:1119	non-lodging accessions	1098:1119	The more lodging-prone indica weedy rice had a smaller bending stress and lower lignin content than non-lodging accessions.					
36478231	2	50	theme	notorious	94:102	arg1	weeds					104:108	the most notorious weeds	85:108	the most notorious weeds of cultivated rice	85:127	, one of the most notorious weeds of cultivated rice, evades eradication through stem lodging and seed shattering.					
36478231	5	51	theme	basal	593:597	arg1	stems					599:603	basal stems	593:603	basal stems	593:603	The chemical composition of basal stems was determined, and transcriptome and methylome sequencing were used to assess the variation in expression of lodging-related genes.					
36478231	8	52	theme	cultivated	1152:1161	arg1	rice					1163:1166	co-occurring cultivated rice	1139:1166	co-occurring cultivated rice	1139:1166	In comparison to co-occurring cultivated rice, there was a lower ratio of cellulose to lignin content in the lodging-prone weedy rice.					
36478231	7	53	theme	smaller	1045:1051	arg1	stress					1061:1066	a smaller bending stress	1043:1066	a smaller bending stress	1043:1066	The more lodging-prone indica weedy rice had a smaller bending stress and lower lignin content than non-lodging accessions.					
36478231	6	54	theme	lower	935:939	arg1	levels					949:954	lower lodging levels	935:954	lower lodging levels	935:954	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	10	55	theme	weedy	1588:1592	arg1	rice					1594:1597	weedy rice	1588:1597	weedy rice	1588:1597	Taken together, our results show that DNA methylation in lignin-related genes regulates variations in stem strength and lodging in weedy rice, and between weed strains and co-occurring cultivated rice.					
36478231	7	56	theme	indica	1021:1026	arg1	rice					1034:1037	The more lodging-prone indica weedy rice	998:1037	The more lodging-prone indica weedy rice	998:1037	The more lodging-prone indica weedy rice had a smaller bending stress and lower lignin content than non-lodging accessions.					
36478231	9	57	theme	cellulose	1428:1436	arg1	ratio					1419:1423	the ratio	1415:1423	the ratio of cellulose to lignin content	1415:1454	Variation in DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3 mediated the differences in their expression levels and affected the ratio of cellulose to lignin content.					
36478231	9	58	from	Variation	1257:1265	arg1	methylation					1274:1284	DNA methylation	1270:1284	DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3	1270:1348	Variation in DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3 mediated the differences in their expression levels and affected the ratio of cellulose to lignin content.					
36478231	3	59	dep	focused	209:215	arg1	whereas					237:243	whereas	237:243	whereas	237:243	Many studies have focused on seed shattering, whereas variations in lodging have received less attention and the underlying mechanisms that cause the differences in lodging between weedy and cultivated rice have not been studied in detail.					
36478231	5	60	from	variation	688:696	arg1	expression					701:710	expression	701:710	expression of lodging-related genes	701:735	The chemical composition of basal stems was determined, and transcriptome and methylome sequencing were used to assess the variation in expression of lodging-related genes.					
36478231	9	61	theme	lignin	1441:1446	arg1	content					1448:1454	lignin content	1441:1454	lignin content	1441:1454	Variation in DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3 mediated the differences in their expression levels and affected the ratio of cellulose to lignin content.					
36478231	8	62	theme	lower	1181:1185	arg1	ratio					1187:1191	a lower ratio	1179:1191	a lower ratio of cellulose to lignin content in the lodging-prone weedy rice	1179:1254	In comparison to co-occurring cultivated rice, there was a lower ratio of cellulose to lignin content in the lodging-prone weedy rice.					
36478231	8	63	theme	weedy	1245:1249	arg1	rice					1251:1254	the lodging-prone weedy rice	1227:1254	the lodging-prone weedy rice	1227:1254	In comparison to co-occurring cultivated rice, there was a lower ratio of cellulose to lignin content in the lodging-prone weedy rice.					
36478231	5	64	theme	methylome	643:651	arg1	sequencing					653:662	methylome sequencing	643:662	methylome sequencing	643:662	The chemical composition of basal stems was determined, and transcriptome and methylome sequencing were used to assess the variation in expression of lodging-related genes.					
36478231	4	65	theme	lodging	449:455	arg1	variation					457:465	lodging variation	449:465	lodging variation among diverse Chinese weedy rice strains and between weedy rice and co-occurring cultivated rice	449:562	Here, we compared lodging variation among diverse Chinese weedy rice strains and between weedy rice and co-occurring cultivated rice.					
36478231	5	66	theme	genes	731:735	arg1	expression					701:710	expression	701:710	expression of lodging-related genes	701:735	The chemical composition of basal stems was determined, and transcriptome and methylome sequencing were used to assess the variation in expression of lodging-related genes.					
36478231	8	67	from	content	1216:1222	arg1	rice					1251:1254	the lodging-prone weedy rice	1227:1254	the lodging-prone weedy rice	1227:1254	In comparison to co-occurring cultivated rice, there was a lower ratio of cellulose to lignin content in the lodging-prone weedy rice.					
36478231	6	68	located	found	968:972	arg2	levels					949:954	lower lodging levels	935:954	lower lodging levels	935:954	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	6	68	located	found	968:972	arg1	north					991:995	the north	987:995	the north	987:995	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	9	69	theme	DNA	1270:1272	arg1	methylation					1274:1284	DNA methylation	1270:1284	DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3	1270:1348	Variation in DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3 mediated the differences in their expression levels and affected the ratio of cellulose to lignin content.					
36478231	5	70	theme	chemical	569:576	arg1	composition					578:588	The chemical composition	565:588	The chemical composition of basal stems	565:603	The chemical composition of basal stems was determined, and transcriptome and methylome sequencing were used to assess the variation in expression of lodging-related genes.					
36478231	3	71	theme	underlying	304:313	arg1	mechanisms					315:324	the underlying mechanisms	300:324	the underlying mechanisms that cause the differences in lodging between weedy and cultivated rice	300:396	Many studies have focused on seed shattering, whereas variations in lodging have received less attention and the underlying mechanisms that cause the differences in lodging between weedy and cultivated rice have not been studied in detail.					
36478231	0	72	theme	variation	28:36	arg1	Characterization					0:15	Characterization	0:15	Characterization of lodging variation of weedy rice.	0:51	Characterization of lodging variation of weedy rice.					
36478231	9	73	theme	synthesis-related	1296:1312	arg1	OsSWN1					1314:1319	lignin synthesis-related OsSWN1	1289:1319	lignin synthesis-related OsSWN1	1289:1319	Variation in DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3 mediated the differences in their expression levels and affected the ratio of cellulose to lignin content.					
36478231	4	74	theme	weedy	520:524	arg1	rice					526:529	weedy rice	520:529	weedy rice	520:529	Here, we compared lodging variation among diverse Chinese weedy rice strains and between weedy rice and co-occurring cultivated rice.					
36478231	7	75	contain	had	1039:1041	arg2	stress					1061:1066	a smaller bending stress	1043:1066	a smaller bending stress	1043:1066	The more lodging-prone indica weedy rice had a smaller bending stress and lower lignin content than non-lodging accessions.					
36478231	7	75	contain	had	1039:1041	arg1	rice					1034:1037	The more lodging-prone indica weedy rice	998:1037	The more lodging-prone indica weedy rice	998:1037	The more lodging-prone indica weedy rice had a smaller bending stress and lower lignin content than non-lodging accessions.					
36478231	0	76	theme	weedy	41:45	arg1	rice					47:50	weedy rice	41:50	weedy rice	41:50	Characterization of lodging variation of weedy rice.					
36478231	9	77	from	differences	1363:1373	arg1	levels					1395:1400	their expression levels	1378:1400	their expression levels	1378:1400	Variation in DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3 mediated the differences in their expression levels and affected the ratio of cellulose to lignin content.					
36478231	6	78	theme	high	832:835	arg1	levels					845:850	high lodging levels	832:850	high lodging levels	832:850	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	4	79	theme	Chinese	481:487	arg1	strains					500:506	diverse Chinese weedy rice strains	473:506	diverse Chinese weedy rice strains	473:506	Here, we compared lodging variation among diverse Chinese weedy rice strains and between weedy rice and co-occurring cultivated rice.					
36478231	10	80	theme	weed	1612:1615	arg1	strains					1617:1623	weed strains	1612:1623	weed strains	1612:1623	Taken together, our results show that DNA methylation in lignin-related genes regulates variations in stem strength and lodging in weedy rice, and between weed strains and co-occurring cultivated rice.					
36478231	4	81	theme	rice	495:498	arg1	strains					500:506	diverse Chinese weedy rice strains	473:506	diverse Chinese weedy rice strains	473:506	Here, we compared lodging variation among diverse Chinese weedy rice strains and between weedy rice and co-occurring cultivated rice.					
36478231	6	82	theme	indica-derived	799:812	arg1	strains					819:825	indica-derived weed strains	799:825	indica-derived weed strains with high lodging levels, which occurred predominantly in southern China,	799:899	The results showed that the degree of lodging varied between indica-derived weed strains with high lodging levels, which occurred predominantly in southern China, and japonica-derived strains with lower lodging levels, which were found primarily in the north.					
36478231	2	83	theme	stem	157:160	arg1	lodging					162:168	stem lodging	157:168	stem lodging	157:168	, one of the most notorious weeds of cultivated rice, evades eradication through stem lodging and seed shattering.					
36478231	10	84	theme	cultivated	1642:1651	arg1	rice					1653:1656	co-occurring cultivated rice	1629:1656	co-occurring cultivated rice	1629:1656	Taken together, our results show that DNA methylation in lignin-related genes regulates variations in stem strength and lodging in weedy rice, and between weed strains and co-occurring cultivated rice.					
36478231	9	85	theme	Os4CL3	1343:1348	arg1	methylation					1274:1284	DNA methylation	1270:1284	DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3	1270:1348	Variation in DNA methylation of lignin synthesis-related OsSWN1, OsMYBX9, OsPAL1, and Os4CL3 mediated the differences in their expression levels and affected the ratio of cellulose to lignin content.					
36478231	10	86	theme	DNA	1495:1497	arg1	methylation					1499:1509	DNA methylation	1495:1509	DNA methylation in lignin-related genes	1495:1533	Taken together, our results show that DNA methylation in lignin-related genes regulates variations in stem strength and lodging in weedy rice, and between weed strains and co-occurring cultivated rice.					
36478231	2	87	theme	most	89:92	arg1	weeds					104:108	the most notorious weeds	85:108	the most notorious weeds of cultivated rice	85:127	, one of the most notorious weeds of cultivated rice, evades eradication through stem lodging and seed shattering.					
36630012	4	0	theme	Raman	822:826	arg1	Spectroscopy					828:839	Raman Spectroscopy	822:839	Raman Spectroscopy	822:839	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	8	1	theme	55°-	1242:1245	arg1	65°					1247:1249	55°- 65°	1242:1249	55°- 65°	1242:1249	Desired wettability was achieved as the contact angles of coated samples were in the range of 55°- 65°.					
36630012	8	2	theme	Desired	1148:1154	arg1	wettability					1156:1166	Desired wettability	1148:1166	Desired wettability	1148:1166	Desired wettability was achieved as the contact angles of coated samples were in the range of 55°- 65°.					
36630012	3	3	theme	Mg	566:567	arg1	Mg-AC					576:580	Mg-AC	576:580	Mg-AC	576:580	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	3	theme	Mg	566:567	arg1	alloy					569:573	(d) CS-coated anodized Mg alloy	543:573	(d) CS-coated anodized Mg alloy (Mg-AC)	543:581	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	4	theme	Anodized	486:493	arg1	Mg-A					505:508	Mg-A	505:508	Mg-A	505:508	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	4	theme	Anodized	486:493	arg1	alloy					498:502	(b) Anodized Mg alloy	482:502	(b) Anodized Mg alloy (Mg-A)	482:509	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	8	5	theme	contact	1188:1194	arg1	angles					1196:1201	the contact angles	1184:1201	the contact angles of coated samples	1184:1219	Desired wettability was achieved as the contact angles of coated samples were in the range of 55°- 65°.					
36630012	3	6	theme	Mg	495:496	arg1	Mg-A					505:508	Mg-A	505:508	Mg-A	505:508	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	6	theme	Mg	495:496	arg1	alloy					498:502	(b) Anodized Mg alloy	482:502	(b) Anodized Mg alloy (Mg-A)	482:509	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	7	theme	Mg	467:468	arg1	alloy					470:474	(a) bare Mg alloy	458:474	(a) bare Mg alloy (Mg)	458:479	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	7	theme	Mg	467:468	arg1	Mg					477:478	Mg	477:478	Mg	477:478	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	8	theme	fabricated	360:369	arg1	coatings					371:378	various fabricated coatings	352:378	various fabricated coatings (with and without pre- anodizing)	352:412	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	4	9	theme	Emission	682:689	arg1	FE-SEM					722:727	FE-SEM	722:727	FE-SEM	722:727	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	4	9	theme	Emission	682:689	arg1	Microscopes					709:719	Field Emission Scanning Electron Microscopes	676:719	Field Emission Scanning Electron Microscopes (FE-SEM)	676:728	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	10	10	theme	sample	1503:1508	arg1	surface					1488:1494	the entire surface	1477:1494	the entire surface of the sample	1477:1508	The composite coated sample showed improved cell adhesion since the osteoblast cells covered almost the entire surface of the sample.					
36630012	3	11	dep	alloy	470:474	arg1	a					459:459	a	459:459	a	459:459	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	1	12	theme	bone	184:187	arg1	applications					202:213	bone regeneration applications	184:213	bone regeneration applications	184:213	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	1	12	theme	bone	184:187	arg1	implants					168:175	biodegradable orthopedic implants	143:175	biodegradable orthopedic implants (e.g., bone regeneration applications)	143:214	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	4	13	theme	Infrared	787:794	arg1	FT-IR					810:814	FT-IR	810:814	FT-IR	810:814	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	4	13	theme	Infrared	787:794	arg1	Spectroscopy					796:807	Infrared Spectroscopy	787:807	Infrared Spectroscopy (FT-IR)	787:815	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	4	14	theme	Field	676:680	arg1	FE-SEM					722:727	FE-SEM	722:727	FE-SEM	722:727	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	4	14	theme	Field	676:680	arg1	Microscopes					709:719	Field Emission Scanning Electron Microscopes	676:719	Field Emission Scanning Electron Microscopes (FE-SEM)	676:728	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	8	15	theme	samples	1213:1219	arg1	angles					1196:1201	the contact angles	1184:1201	the contact angles of coated samples	1184:1219	Desired wettability was achieved as the contact angles of coated samples were in the range of 55°- 65°.					
36630012	1	16	theme	regeneration	189:200	arg1	applications					202:213	bone regeneration applications	184:213	bone regeneration applications	184:213	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	1	16	theme	regeneration	189:200	arg1	implants					168:175	biodegradable orthopedic implants	143:175	biodegradable orthopedic implants (e.g., bone regeneration applications)	143:214	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	3	17	theme	pre-	398:401	arg1	anodizing					403:411	pre- anodizing	398:411	pre- anodizing	398:411	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	4	18	theme	Electron	700:707	arg1	FE-SEM					722:727	FE-SEM	722:727	FE-SEM	722:727	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	4	18	theme	Electron	700:707	arg1	Microscopes					709:719	Field Emission Scanning Electron Microscopes	676:719	Field Emission Scanning Electron Microscopes (FE-SEM)	676:728	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	7	19	theme	adhesion	1044:1051	arg1	test					1053:1056	The adhesion test	1040:1056	The adhesion test	1040:1056	The adhesion test showed that the coatings exhibited higher adhesion for Mg-AC and Mg-ACC compared to Mg-C.					
36630012	1	20	theme	Mg-based	100:107	arg1	biomaterials					109:120	Mg-based biomaterials	100:120	Mg-based biomaterials	100:120	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	1	20	theme	Mg-based	100:107	arg1	implants					168:175	biodegradable orthopedic implants	143:175	biodegradable orthopedic implants (e.g., bone regeneration applications)	143:214	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	7	21	theme	higher	1093:1098	arg1	adhesion					1100:1107	higher adhesion	1093:1107	higher adhesion	1093:1107	The adhesion test showed that the coatings exhibited higher adhesion for Mg-AC and Mg-ACC compared to Mg-C.					
36630012	9	22	theme	Electrochemical	1252:1266	arg1	impedance					1268:1276	Electrochemical impedance	1252:1276	Electrochemical impedance	1252:1276	Electrochemical impedance and polarization as well as immersion tests showed higher corrosion resistance for coated samples.					
36630012	3	23	dep	alloy	569:573	arg1	d					544:544	d	544:544	d	544:544	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	24	theme	CS-CNT-coated	592:604	arg1	alloy					618:622	(e) CS-CNT-coated anodized Mg alloy	588:622	(e) CS-CNT-coated anodized Mg alloy (Mg-ACC)	588:631	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	24	theme	CS-CNT-coated	592:604	arg1	Mg-ACC					625:630	Mg-ACC	625:630	Mg-ACC	625:630	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	4	25	theme	Scanning	691:698	arg1	FE-SEM					722:727	FE-SEM	722:727	FE-SEM	722:727	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	4	25	theme	Scanning	691:698	arg1	Microscopes					709:719	Field Emission Scanning Electron Microscopes	676:719	Field Emission Scanning Electron Microscopes (FE-SEM)	676:728	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	3	26	theme	CS-coated	547:555	arg1	Mg-AC					576:580	Mg-AC	576:580	Mg-AC	576:580	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	26	theme	CS-coated	547:555	arg1	alloy					569:573	(d) CS-coated anodized Mg alloy	543:573	(d) CS-coated anodized Mg alloy (Mg-AC)	543:581	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	27	theme	samples	434:440	arg1	categories					420:429	five categories	415:429	five categories of samples	415:440	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	0	28	theme	chitosan/carbon	31:45	arg1	coatings					56:63	electrospun chitosan/carbon nanotube coatings	19:63	electrospun chitosan/carbon nanotube coatings	19:63	Characteristics of electrospun chitosan/carbon nanotube coatings deposited on AZ31 magnesium alloy.					
36630012	3	29	theme	anodized	606:613	arg1	alloy					618:622	(e) CS-CNT-coated anodized Mg alloy	588:622	(e) CS-CNT-coated anodized Mg alloy (Mg-ACC)	588:631	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	29	theme	anodized	606:613	arg1	Mg-ACC					625:630	Mg-ACC	625:630	Mg-ACC	625:630	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	30	dep	considered	446:455	arg1	Mg-C					536:539	Mg-C	536:539	Mg-C	536:539	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	30	dep	considered	446:455	arg1	alloy					498:502	(b) Anodized Mg alloy	482:502	(b) Anodized Mg alloy (Mg-A)	482:509	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	30	dep	considered	446:455	arg1	alloy					618:622	(e) CS-CNT-coated anodized Mg alloy	588:622	(e) CS-CNT-coated anodized Mg alloy (Mg-ACC)	588:631	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	30	dep	considered	446:455	arg1	Mg-A					505:508	Mg-A	505:508	Mg-A	505:508	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	30	dep	considered	446:455	arg1	alloy					569:573	(d) CS-coated anodized Mg alloy	543:573	(d) CS-coated anodized Mg alloy (Mg-AC)	543:581	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	30	dep	considered	446:455	arg1	Mg-ACC					625:630	Mg-ACC	625:630	Mg-ACC	625:630	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	30	dep	considered	446:455	arg1	alloy					470:474	(a) bare Mg alloy	458:474	(a) bare Mg alloy (Mg)	458:479	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	30	dep	considered	446:455	arg1	Mg-AC					576:580	Mg-AC	576:580	Mg-AC	576:580	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	30	dep	considered	446:455	arg1	alloy					529:533	(c) CS-coated Mg alloy	512:533	(c) CS-coated Mg alloy (Mg-C)	512:540	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	30	dep	considered	446:455	arg1	Mg					477:478	Mg	477:478	Mg	477:478	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	5	31	theme	coated	866:871	arg1	samples					873:879	the coated samples	862:879	the coated samples	862:879	The adhesion within the coated samples was compared.					
36630012	3	32	theme	c	513:513	arg1	Mg-C					536:539	Mg-C	536:539	Mg-C	536:539	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	32	theme	c	513:513	arg1	alloy					529:533	(c) CS-coated Mg alloy	512:533	(c) CS-coated Mg alloy (Mg-C)	512:540	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	0	33	theme	electrospun	19:29	arg1	coatings					56:63	electrospun chitosan/carbon nanotube coatings	19:63	electrospun chitosan/carbon nanotube coatings	19:63	Characteristics of electrospun chitosan/carbon nanotube coatings deposited on AZ31 magnesium alloy.					
36630012	3	34	theme	e	589:589	arg1	alloy					618:622	(e) CS-CNT-coated anodized Mg alloy	588:622	(e) CS-CNT-coated anodized Mg alloy (Mg-ACC)	588:631	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	34	theme	e	589:589	arg1	Mg-ACC					625:630	Mg-ACC	625:630	Mg-ACC	625:630	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	11	35	theme	osteoblast	1521:1530	arg1	viability					1537:1545	osteoblast cell viability	1521:1545	osteoblast cell viability for the sample	1521:1560	Moreover, osteoblast cell viability for the sample was around 40% higher than that of the bare sample.					
36630012	1	36	used	used	135:138	arg2	biomaterials					109:120	Mg-based biomaterials	100:120	Mg-based biomaterials	100:120	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	1	36	used	used	135:138	arg2	implants					168:175	biodegradable orthopedic implants	143:175	biodegradable orthopedic implants (e.g., bone regeneration applications)	143:214	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	6	37	theme	corrosion	970:978	arg1	behavior					980:987	corrosion behavior	970:987	corrosion behavior	970:987	Then, the effects of the coatings were evaluated by comparing wettability, corrosion behavior, and biocompatibility for bare and coated samples.					
36630012	0	38	theme	coatings	56:63	arg1	Characteristics					0:14	Characteristics	0:14	Characteristics of electrospun chitosan/carbon nanotube coatings	0:63	Characteristics of electrospun chitosan/carbon nanotube coatings deposited on AZ31 magnesium alloy.					
36630012	11	39	theme	bare	1601:1604	arg1	sample					1606:1611	the bare sample	1597:1611	the bare sample	1597:1611	Moreover, osteoblast cell viability for the sample was around 40% higher than that of the bare sample.					
36630012	8	40	theme	65°	1247:1249	arg1	range					1233:1237	the range	1229:1237	the range of 55°- 65°	1229:1249	Desired wettability was achieved as the contact angles of coated samples were in the range of 55°- 65°.					
36630012	1	41	dep	applications	202:213	arg1	e.g.					178:181	e.g.	178:181	e.g.	178:181	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	10	42	theme	cell	1421:1424	arg1	adhesion					1426:1433	improved cell adhesion	1412:1433	improved cell adhesion	1412:1433	The composite coated sample showed improved cell adhesion since the osteoblast cells covered almost the entire surface of the sample.					
36630012	0	43	theme	nanotube	47:54	arg1	coatings					56:63	electrospun chitosan/carbon nanotube coatings	19:63	electrospun chitosan/carbon nanotube coatings	19:63	Characteristics of electrospun chitosan/carbon nanotube coatings deposited on AZ31 magnesium alloy.					
36630012	4	44	dep	Fourier	769:775	arg1	Transform					777:785	Transform	777:785	Transform Infrared Spectroscopy (FT-IR)	777:815	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	8	45	theme	coated	1206:1211	arg1	samples					1213:1219	coated samples	1206:1219	coated samples	1206:1219	Desired wettability was achieved as the contact angles of coated samples were in the range of 55°- 65°.					
36630012	10	46	theme	composite	1381:1389	arg1	sample					1398:1403	The composite coated sample	1377:1403	The composite coated sample	1377:1403	The composite coated sample showed improved cell adhesion since the osteoblast cells covered almost the entire surface of the sample.					
36630012	10	47	theme	osteoblast	1445:1454	arg1	cells					1456:1460	the osteoblast cells	1441:1460	the osteoblast cells	1441:1460	The composite coated sample showed improved cell adhesion since the osteoblast cells covered almost the entire surface of the sample.					
36630012	4	48	theme	Energy	731:736	arg1	EDS					763:765	EDS	763:765	EDS	763:765	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	4	48	theme	Energy	731:736	arg1	Spectroscopy					749:760	Energy Dispersive Spectroscopy	731:760	Energy Dispersive Spectroscopy (EDS)	731:766	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	3	49	theme	various	352:358	arg1	coatings					371:378	various fabricated coatings	352:378	various fabricated coatings (with and without pre- anodizing)	352:412	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	6	50	theme	coatings	920:927	arg1	effects					905:911	the effects	901:911	the effects of the coatings	901:927	Then, the effects of the coatings were evaluated by comparing wettability, corrosion behavior, and biocompatibility for bare and coated samples.					
36630012	3	51	theme	Mg	615:616	arg1	alloy					618:622	(e) CS-CNT-coated anodized Mg alloy	588:622	(e) CS-CNT-coated anodized Mg alloy (Mg-ACC)	588:631	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	51	theme	Mg	615:616	arg1	Mg-ACC					625:630	Mg-ACC	625:630	Mg-ACC	625:630	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	2	52	theme	achieving	226:234	arg1	biocompatibility					241:256	high biocompatibility	236:256	high biocompatibility	236:256	However, achieving high biocompatibility and corrosion resistance has remained a challenge to be tackled.					
36630012	2	53	theme	high	236:239	arg1	biocompatibility					241:256	high biocompatibility	236:256	high biocompatibility	236:256	However, achieving high biocompatibility and corrosion resistance has remained a challenge to be tackled.					
36630012	9	54	theme	immersion	1306:1314	arg1	tests					1316:1320	immersion tests	1306:1320	Electrochemical impedance and polarization as well as immersion tests	1252:1320	Electrochemical impedance and polarization as well as immersion tests showed higher corrosion resistance for coated samples.					
36630012	10	55	theme	improved	1412:1419	arg1	adhesion					1426:1433	improved cell adhesion	1412:1433	improved cell adhesion	1412:1433	The composite coated sample showed improved cell adhesion since the osteoblast cells covered almost the entire surface of the sample.					
36630012	6	56	theme	bare	1015:1018	arg1	samples					1031:1037	bare and coated samples	1015:1037	bare and coated samples	1015:1037	Then, the effects of the coatings were evaluated by comparing wettability, corrosion behavior, and biocompatibility for bare and coated samples.					
36630012	11	57	theme	cell	1532:1535	arg1	viability					1537:1545	osteoblast cell viability	1521:1545	osteoblast cell viability for the sample	1521:1560	Moreover, osteoblast cell viability for the sample was around 40% higher than that of the bare sample.					
36630012	3	58	theme	anodized	557:564	arg1	Mg-AC					576:580	Mg-AC	576:580	Mg-AC	576:580	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	58	theme	anodized	557:564	arg1	alloy					569:573	(d) CS-coated anodized Mg alloy	543:573	(d) CS-coated anodized Mg alloy (Mg-AC)	543:581	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	1	59	theme	biodegradable	143:155	arg1	applications					202:213	bone regeneration applications	184:213	bone regeneration applications	184:213	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	1	59	theme	biodegradable	143:155	arg1	biomaterials					109:120	Mg-based biomaterials	100:120	Mg-based biomaterials	100:120	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	1	59	theme	biodegradable	143:155	arg1	implants					168:175	biodegradable orthopedic implants	143:175	biodegradable orthopedic implants (e.g., bone regeneration applications)	143:214	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	10	60	theme	coated	1391:1396	arg1	sample					1398:1403	The composite coated sample	1377:1403	The composite coated sample	1377:1403	The composite coated sample showed improved cell adhesion since the osteoblast cells covered almost the entire surface of the sample.					
36630012	4	61	theme	Dispersive	738:747	arg1	EDS					763:765	EDS	763:765	EDS	763:765	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	4	61	theme	Dispersive	738:747	arg1	Spectroscopy					749:760	Energy Dispersive Spectroscopy	731:760	Energy Dispersive Spectroscopy (EDS)	731:766	These samples were characterized by using Field Emission Scanning Electron Microscopes (FE-SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), and Raman Spectroscopy.					
36630012	9	62	theme	coated	1361:1366	arg1	samples					1368:1374	coated samples	1361:1374	coated samples	1361:1374	Electrochemical impedance and polarization as well as immersion tests showed higher corrosion resistance for coated samples.					
36630012	6	63	theme	coated	1024:1029	arg1	samples					1031:1037	bare and coated samples	1015:1037	bare and coated samples	1015:1037	Then, the effects of the coatings were evaluated by comparing wettability, corrosion behavior, and biocompatibility for bare and coated samples.					
36630012	1	64	theme	orthopedic	157:166	arg1	applications					202:213	bone regeneration applications	184:213	bone regeneration applications	184:213	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	1	64	theme	orthopedic	157:166	arg1	biomaterials					109:120	Mg-based biomaterials	100:120	Mg-based biomaterials	100:120	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	1	64	theme	orthopedic	157:166	arg1	implants					168:175	biodegradable orthopedic implants	143:175	biodegradable orthopedic implants (e.g., bone regeneration applications)	143:214	Mg-based biomaterials are commonly used as biodegradable orthopedic implants (e.g., bone regeneration applications).					
36630012	3	65	theme	bare	462:465	arg1	alloy					470:474	(a) bare Mg alloy	458:474	(a) bare Mg alloy (Mg)	458:479	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	65	theme	bare	462:465	arg1	Mg					477:478	Mg	477:478	Mg	477:478	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	0	66	theme	magnesium	83:91	arg1	alloy					93:97	AZ31 magnesium alloy	78:97	AZ31 magnesium alloy	78:97	Characteristics of electrospun chitosan/carbon nanotube coatings deposited on AZ31 magnesium alloy.					
36630012	3	67	theme	CS-coated	516:524	arg1	Mg-C					536:539	Mg-C	536:539	Mg-C	536:539	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	67	theme	CS-coated	516:524	arg1	alloy					529:533	(c) CS-coated Mg alloy	512:533	(c) CS-coated Mg alloy (Mg-C)	512:540	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	9	68	theme	higher	1329:1334	arg1	resistance					1346:1355	higher corrosion resistance	1329:1355	higher corrosion resistance for coated samples	1329:1374	Electrochemical impedance and polarization as well as immersion tests showed higher corrosion resistance for coated samples.					
36630012	3	69	dep	alloy	498:502	arg1	b					483:483	b	483:483	b	483:483	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	10	70	theme	entire	1481:1486	arg1	surface					1488:1494	the entire surface	1477:1494	the entire surface of the sample	1477:1508	The composite coated sample showed improved cell adhesion since the osteoblast cells covered almost the entire surface of the sample.					
36630012	0	71	theme	AZ31	78:81	arg1	alloy					93:97	AZ31 magnesium alloy	78:97	AZ31 magnesium alloy	78:97	Characteristics of electrospun chitosan/carbon nanotube coatings deposited on AZ31 magnesium alloy.					
36630012	3	72	theme	Mg	526:527	arg1	Mg-C					536:539	Mg-C	536:539	Mg-C	536:539	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	3	72	theme	Mg	526:527	arg1	alloy					529:533	(c) CS-coated Mg alloy	512:533	(c) CS-coated Mg alloy (Mg-C)	512:540	In this work, to investigate various fabricated coatings (with and without pre- anodizing), five categories of samples are considered: (a) bare Mg alloy (Mg), (b) Anodized Mg alloy (Mg-A), (c) CS-coated Mg alloy (Mg-C), (d) CS-coated anodized Mg alloy (Mg-AC), and (e) CS-CNT-coated anodized Mg alloy (Mg-ACC).					
36630012	2	73	theme	corrosion	262:270	arg1	resistance					272:281	corrosion resistance	262:281	corrosion resistance	262:281	However, achieving high biocompatibility and corrosion resistance has remained a challenge to be tackled.					
36630012	9	74	theme	corrosion	1336:1344	arg1	resistance					1346:1355	higher corrosion resistance	1329:1355	higher corrosion resistance for coated samples	1329:1374	Electrochemical impedance and polarization as well as immersion tests showed higher corrosion resistance for coated samples.					
35998726	0	0	theme	lead	94:97	arg1	removal					83:89	selective and highly efficient removal	52:89	selective and highly efficient removal of lead and cadmium from wastewater	52:125	Amino-modified chitosan/gold tailings composite for selective and highly efficient removal of lead and cadmium from wastewater.					
35998726	3	1	theme	192.78 mg·g-1	580:592	arg1	capacity					568:575	maximum adsorption capacity	549:575	maximum adsorption capacity of 192.78 mg·g-1 and 99.46 mg·g-1 for Pb(Ⅱ) and Cd(Ⅱ)	549:629	The results showed that CS-PEI-nGT had maximum adsorption capacity of 192.78 mg·g-1 and 99.46 mg·g-1 for Pb(Ⅱ) and Cd(Ⅱ) respectively at pH 5.					
35998726	4	2	theme	kinetic	719:725	arg1	model					738:742	pseudo-second-order kinetic adsorption model	699:742	pseudo-second-order kinetic adsorption model	699:742	The adsorption kinetics was described well by pseudo-second-order kinetic adsorption model, and suggested that chemisorption as the rate-controlling step for adsorption of Pb(Ⅱ) and Cd(Ⅱ).					
35998726	1	3	theme	novel	144:148	arg1	CS-PEI-nGT					194:203	CS-PEI-nGT	194:203	CS-PEI-nGT	194:203	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	1	3	theme	novel	144:148	arg1	composite					183:191	a novel amino-modified chitosan/tailings composite	142:191	a novel amino-modified chitosan/tailings composite (CS-PEI-nGT)	142:204	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	7	4	theme	composite	1312:1320	arg1	surface					1322:1328	composite surface	1312:1328	composite surface	1312:1328	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	8	5	from	wastewater	1565:1574	arg1	recycling					1500:1508	tailings recycling	1491:1508	tailings recycling	1491:1508	Our work presents a promising strategy for tailings recycling and highly efficient removal of toxic metals ions from wastewater.					
35998726	8	5	from	wastewater	1565:1574	arg1	removal					1531:1537	highly efficient removal	1514:1537	highly efficient removal of toxic metals ions from wastewater	1514:1574	Our work presents a promising strategy for tailings recycling and highly efficient removal of toxic metals ions from wastewater.					
35998726	6	6	theme	other	1099:1103	arg1	Mg					1134:1135	Mg	1134:1135	Mg(Ⅱ)	1134:1138	In addition, CS-PEI-nGT retained its selective adsorption capacity for Pb(Ⅱ) and Cd(Ⅱ), compared to other metals such as Zn(Ⅱ), Mn(Ⅱ), Mg(Ⅱ) and Al(Ⅲ).					
35998726	6	6	theme	other	1099:1103	arg1	metals					1105:1110	other metals	1099:1110	other metals such as Zn(Ⅱ), Mn(Ⅱ), Mg(Ⅱ) and Al(Ⅲ)	1099:1148	In addition, CS-PEI-nGT retained its selective adsorption capacity for Pb(Ⅱ) and Cd(Ⅱ), compared to other metals such as Zn(Ⅱ), Mn(Ⅱ), Mg(Ⅱ) and Al(Ⅲ).					
35998726	6	6	theme	other	1099:1103	arg1	Zn					1120:1121	Zn	1120:1121	Zn(Ⅱ)	1120:1124	In addition, CS-PEI-nGT retained its selective adsorption capacity for Pb(Ⅱ) and Cd(Ⅱ), compared to other metals such as Zn(Ⅱ), Mn(Ⅱ), Mg(Ⅱ) and Al(Ⅲ).					
35998726	6	6	theme	other	1099:1103	arg1	Al					1144:1145	Al	1144:1145	Al(Ⅲ)	1144:1148	In addition, CS-PEI-nGT retained its selective adsorption capacity for Pb(Ⅱ) and Cd(Ⅱ), compared to other metals such as Zn(Ⅱ), Mn(Ⅱ), Mg(Ⅱ) and Al(Ⅲ).					
35998726	6	6	theme	other	1099:1103	arg1	Mn					1127:1128	Mn	1127:1128	Mn(Ⅱ)	1127:1131	In addition, CS-PEI-nGT retained its selective adsorption capacity for Pb(Ⅱ) and Cd(Ⅱ), compared to other metals such as Zn(Ⅱ), Mn(Ⅱ), Mg(Ⅱ) and Al(Ⅲ).					
35998726	3	7	theme	adsorption	557:566	arg1	capacity					568:575	maximum adsorption capacity	549:575	maximum adsorption capacity of 192.78 mg·g-1 and 99.46 mg·g-1 for Pb(Ⅱ) and Cd(Ⅱ)	549:629	The results showed that CS-PEI-nGT had maximum adsorption capacity of 192.78 mg·g-1 and 99.46 mg·g-1 for Pb(Ⅱ) and Cd(Ⅱ) respectively at pH 5.					
35998726	2	8	theme	BET	481:483	arg1	techniques					498:507	FTIR, XRD, SEM, BET, TGA and XPS techniques	465:507	FTIR, XRD, SEM, BET, TGA and XPS techniques	465:507	The CS-PEI-nGT was characterized by using FTIR, XRD, SEM, BET, TGA and XPS techniques.					
35998726	1	9	theme	amino-modified	150:163	arg1	CS-PEI-nGT					194:203	CS-PEI-nGT	194:203	CS-PEI-nGT	194:203	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	1	9	theme	amino-modified	150:163	arg1	composite					183:191	a novel amino-modified chitosan/tailings composite	142:191	a novel amino-modified chitosan/tailings composite (CS-PEI-nGT)	142:204	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	7	10	theme	functional	1291:1300	arg1	groups					1302:1307	hydroxyl (-OH) functional groups	1276:1307	hydroxyl (-OH) functional groups	1276:1307	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	8	11	theme	promising	1468:1476	arg1	strategy					1478:1485	a promising strategy	1466:1485	a promising strategy for tailings recycling and highly efficient removal of toxic metals ions from wastewater	1466:1574	Our work presents a promising strategy for tailings recycling and highly efficient removal of toxic metals ions from wastewater.					
35998726	0	12	theme	cadmium	103:109	arg1	removal					83:89	selective and highly efficient removal	52:89	selective and highly efficient removal of lead and cadmium from wastewater	52:125	Amino-modified chitosan/gold tailings composite for selective and highly efficient removal of lead and cadmium from wastewater.					
35998726	5	13	theme	0.9911	947:952	arg1	coefficient					927:937	higher correlation coefficient	908:937	higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively	908:996	The isotherm data was accurately explained by Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively.					
35998726	5	13	theme	0.9911	947:952	arg1	R2					940:941	R2	940:941	R2	940:941	The isotherm data was accurately explained by Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively.					
35998726	6	14	theme	adsorption	1046:1055	arg1	capacity					1057:1064	its selective adsorption capacity	1032:1064	its selective adsorption capacity for Pb(Ⅱ) and Cd(Ⅱ)	1032:1084	In addition, CS-PEI-nGT retained its selective adsorption capacity for Pb(Ⅱ) and Cd(Ⅱ), compared to other metals such as Zn(Ⅱ), Mn(Ⅱ), Mg(Ⅱ) and Al(Ⅲ).					
35998726	7	15	theme	surface	1381:1387	arg1	complexation					1389:1400	surface complexation	1381:1400	surface complexation	1381:1400	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	1	16	theme	ball	274:277	arg1	nGT					288:290	nGT	288:290	nGT	288:290	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	1	16	theme	ball	274:277	arg1	milling					279:285	ball milling	274:285	ball milling (nGT)	274:291	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	5	17	theme	higher	908:913	arg1	coefficient					927:937	higher correlation coefficient	908:937	higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively	908:996	The isotherm data was accurately explained by Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively.					
35998726	5	17	theme	higher	908:913	arg1	R2					940:941	R2	940:941	R2	940:941	The isotherm data was accurately explained by Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively.					
35998726	2	18	theme	SEM	476:478	arg1	techniques					498:507	FTIR, XRD, SEM, BET, TGA and XPS techniques	465:507	FTIR, XRD, SEM, BET, TGA and XPS techniques	465:507	The CS-PEI-nGT was characterized by using FTIR, XRD, SEM, BET, TGA and XPS techniques.					
35998726	1	19	theme	Cadmium	375:381	arg1	removal					391:397	Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal	358:397	Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions	358:420	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	7	20	from	groups	1302:1307	arg1	surface					1322:1328	composite surface	1312:1328	composite surface	1312:1328	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	7	21	theme	-OH	1286:1288	arg1	groups					1302:1307	hydroxyl (-OH) functional groups	1276:1307	hydroxyl (-OH) functional groups	1276:1307	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	5	22	theme	correlation	915:925	arg1	coefficient					927:937	higher correlation coefficient	908:937	higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively	908:996	The isotherm data was accurately explained by Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively.					
35998726	5	22	theme	correlation	915:925	arg1	R2					940:941	R2	940:941	R2	940:941	The isotherm data was accurately explained by Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively.					
35998726	1	23	theme	chitosan/tailings	165:181	arg1	CS-PEI-nGT					194:203	CS-PEI-nGT	194:203	CS-PEI-nGT	194:203	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	1	23	theme	chitosan/tailings	165:181	arg1	composite					183:191	a novel amino-modified chitosan/tailings composite	142:191	a novel amino-modified chitosan/tailings composite (CS-PEI-nGT)	142:204	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	1	24	theme	raw	339:341	arg1	materials					343:351	raw materials	339:351	raw materials	339:351	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	2	25	theme	XPS	494:496	arg1	techniques					498:507	FTIR, XRD, SEM, BET, TGA and XPS techniques	465:507	FTIR, XRD, SEM, BET, TGA and XPS techniques	465:507	The CS-PEI-nGT was characterized by using FTIR, XRD, SEM, BET, TGA and XPS techniques.					
35998726	7	26	theme	hydroxyl	1276:1283	arg1	groups					1302:1307	hydroxyl (-OH) functional groups	1276:1307	hydroxyl (-OH) functional groups	1276:1307	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	7	27	theme	amino	1230:1234	arg1	-NH2					1237:1240	amino (-NH2)	1230:1241	amino (-NH2)	1230:1241	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	3	28	theme	99.46 mg·g-1	598:609	arg1	capacity					568:575	maximum adsorption capacity	549:575	maximum adsorption capacity of 192.78 mg·g-1 and 99.46 mg·g-1 for Pb(Ⅱ) and Cd(Ⅱ)	549:629	The results showed that CS-PEI-nGT had maximum adsorption capacity of 192.78 mg·g-1 and 99.46 mg·g-1 for Pb(Ⅱ) and Cd(Ⅱ) respectively at pH 5.					
35998726	8	29	theme	metals	1548:1553	arg1	ions					1555:1558	toxic metals ions	1542:1558	toxic metals ions	1542:1558	Our work presents a promising strategy for tailings recycling and highly efficient removal of toxic metals ions from wastewater.					
35998726	1	30	from	solutions	412:420	arg1	removal					391:397	Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal	358:397	Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions	358:420	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	0	31	theme	Amino-modified	0:13	arg1	chitosan/gold					15:27	Amino-modified chitosan/gold	0:27	Amino-modified chitosan/gold	0:27	Amino-modified chitosan/gold tailings composite for selective and highly efficient removal of lead and cadmium from wastewater.					
35998726	7	32	theme	oxide	1252:1256	arg1	Si-O					1266:1269	Si-O	1266:1269	Si-O	1266:1269	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	7	32	theme	oxide	1252:1256	arg1	groups					1258:1263	silicon oxide groups	1244:1263	silicon oxide groups (Si-O)	1244:1270	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	2	33	theme	TGA	486:488	arg1	techniques					498:507	FTIR, XRD, SEM, BET, TGA and XPS techniques	465:507	FTIR, XRD, SEM, BET, TGA and XPS techniques	465:507	The CS-PEI-nGT was characterized by using FTIR, XRD, SEM, BET, TGA and XPS techniques.					
35998726	5	34	with	model	897:901	arg1	coefficient					927:937	higher correlation coefficient	908:937	higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively	908:996	The isotherm data was accurately explained by Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively.					
35998726	5	34	with	model	897:901	arg1	R2					940:941	R2	940:941	R2	940:941	The isotherm data was accurately explained by Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively.					
35998726	3	35	contain	had	545:547	arg1	CS-PEI-nGT					534:543	CS-PEI-nGT	534:543	CS-PEI-nGT	534:543	The results showed that CS-PEI-nGT had maximum adsorption capacity of 192.78 mg·g-1 and 99.46 mg·g-1 for Pb(Ⅱ) and Cd(Ⅱ) respectively at pH 5.					
35998726	3	35	contain	had	545:547	arg2	capacity					568:575	maximum adsorption capacity	549:575	maximum adsorption capacity of 192.78 mg·g-1 and 99.46 mg·g-1 for Pb(Ⅱ) and Cd(Ⅱ)	549:629	The results showed that CS-PEI-nGT had maximum adsorption capacity of 192.78 mg·g-1 and 99.46 mg·g-1 for Pb(Ⅱ) and Cd(Ⅱ) respectively at pH 5.					
35998726	4	36	theme	Cd	835:836	arg1	adsorption					811:820	adsorption	811:820	adsorption of Pb(Ⅱ) and Cd(Ⅱ)	811:839	The adsorption kinetics was described well by pseudo-second-order kinetic adsorption model, and suggested that chemisorption as the rate-controlling step for adsorption of Pb(Ⅱ) and Cd(Ⅱ).					
35998726	4	37	theme	pseudo-second-order	699:717	arg1	model					738:742	pseudo-second-order kinetic adsorption model	699:742	pseudo-second-order kinetic adsorption model	699:742	The adsorption kinetics was described well by pseudo-second-order kinetic adsorption model, and suggested that chemisorption as the rate-controlling step for adsorption of Pb(Ⅱ) and Cd(Ⅱ).					
35998726	5	38	theme	isotherm	846:853	arg1	data					855:858	The isotherm data	842:858	The isotherm data	842:858	The isotherm data was accurately explained by Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively.					
35998726	8	39	theme	efficient	1521:1529	arg1	removal					1531:1537	highly efficient removal	1514:1537	highly efficient removal of toxic metals ions from wastewater	1514:1574	Our work presents a promising strategy for tailings recycling and highly efficient removal of toxic metals ions from wastewater.					
35998726	0	40	theme	selective	52:60	arg1	removal					83:89	selective and highly efficient removal	52:89	selective and highly efficient removal of lead and cadmium from wastewater	52:125	Amino-modified chitosan/gold tailings composite for selective and highly efficient removal of lead and cadmium from wastewater.					
35998726	7	41	theme	silicon	1244:1250	arg1	Si-O					1266:1269	Si-O	1266:1269	Si-O	1266:1269	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	7	41	theme	silicon	1244:1250	arg1	groups					1258:1263	silicon oxide groups	1244:1263	silicon oxide groups (Si-O)	1244:1270	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	4	42	theme	rate-controlling	785:800	arg1	step					802:805	the rate-controlling step	781:805	the rate-controlling step for adsorption of Pb(Ⅱ) and Cd(Ⅱ)	781:839	The adsorption kinetics was described well by pseudo-second-order kinetic adsorption model, and suggested that chemisorption as the rate-controlling step for adsorption of Pb(Ⅱ) and Cd(Ⅱ).					
35998726	4	42	theme	rate-controlling	785:800	arg1	chemisorption					764:776	that chemisorption	759:776	that chemisorption	759:776	The adsorption kinetics was described well by pseudo-second-order kinetic adsorption model, and suggested that chemisorption as the rate-controlling step for adsorption of Pb(Ⅱ) and Cd(Ⅱ).					
35998726	5	43	theme	Langmuir	888:895	arg1	model					897:901	Langmuir model	888:901	Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively	888:996	The isotherm data was accurately explained by Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively.					
35998726	7	44	theme	accountable	1335:1345	arg1	adsorption					1358:1367	accountable for metals adsorption	1335:1367	accountable for metals adsorption	1335:1367	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	1	45	theme	Lead	358:361	arg1	removal					391:397	Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal	358:397	Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions	358:420	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	5	46	theme	0.9642	958:963	arg1	coefficient					927:937	higher correlation coefficient	908:937	higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively	908:996	The isotherm data was accurately explained by Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively.					
35998726	5	46	theme	0.9642	958:963	arg1	R2					940:941	R2	940:941	R2	940:941	The isotherm data was accurately explained by Langmuir model with higher correlation coefficient (R2) of 0.9911 and 0.9642 for Pb(Ⅱ) and Cd(Ⅱ) respectively.					
35998726	8	47	theme	ions	1555:1558	arg1	recycling					1500:1508	tailings recycling	1491:1508	tailings recycling	1491:1508	Our work presents a promising strategy for tailings recycling and highly efficient removal of toxic metals ions from wastewater.					
35998726	8	47	theme	ions	1555:1558	arg1	removal					1531:1537	highly efficient removal	1514:1537	highly efficient removal of toxic metals ions from wastewater	1514:1574	Our work presents a promising strategy for tailings recycling and highly efficient removal of toxic metals ions from wastewater.					
35998726	4	48	theme	Pb	825:826	arg1	adsorption					811:820	adsorption	811:820	adsorption of Pb(Ⅱ) and Cd(Ⅱ)	811:839	The adsorption kinetics was described well by pseudo-second-order kinetic adsorption model, and suggested that chemisorption as the rate-controlling step for adsorption of Pb(Ⅱ) and Cd(Ⅱ).					
35998726	7	49	theme	adsorption	1172:1181	arg1	mechanism					1155:1163	The mechanism	1151:1163	The mechanism of the adsorption	1151:1181	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	6	50	theme	selective	1036:1044	arg1	capacity					1057:1064	its selective adsorption capacity	1032:1064	its selective adsorption capacity for Pb(Ⅱ) and Cd(Ⅱ)	1032:1084	In addition, CS-PEI-nGT retained its selective adsorption capacity for Pb(Ⅱ) and Cd(Ⅱ), compared to other metals such as Zn(Ⅱ), Mn(Ⅱ), Mg(Ⅱ) and Al(Ⅲ).					
35998726	2	51	theme	XRD	471:473	arg1	techniques					498:507	FTIR, XRD, SEM, BET, TGA and XPS techniques	465:507	FTIR, XRD, SEM, BET, TGA and XPS techniques	465:507	The CS-PEI-nGT was characterized by using FTIR, XRD, SEM, BET, TGA and XPS techniques.					
35998726	0	52	from	wastewater	116:125	arg1	removal					83:89	selective and highly efficient removal	52:89	selective and highly efficient removal of lead and cadmium from wastewater	52:125	Amino-modified chitosan/gold tailings composite for selective and highly efficient removal of lead and cadmium from wastewater.					
35998726	4	53	theme	adsorption	657:666	arg1	kinetics					668:675	The adsorption kinetics	653:675	The adsorption kinetics	653:675	The adsorption kinetics was described well by pseudo-second-order kinetic adsorption model, and suggested that chemisorption as the rate-controlling step for adsorption of Pb(Ⅱ) and Cd(Ⅱ).					
35998726	7	54	from	-NH2	1237:1240	arg1	surface					1322:1328	composite surface	1312:1328	composite surface	1312:1328	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	1	55	dep	Cadmium	375:381	arg1	Ⅱ					387:387	Ⅱ	387:387	Ⅱ	387:387	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	1	55	dep	Cadmium	375:381	arg1	Cd					384:385	Cd	384:385	Cd(Ⅱ)	384:388	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	3	56	theme	maximum	549:555	arg1	capacity					568:575	maximum adsorption capacity	549:575	maximum adsorption capacity of 192.78 mg·g-1 and 99.46 mg·g-1 for Pb(Ⅱ) and Cd(Ⅱ)	549:629	The results showed that CS-PEI-nGT had maximum adsorption capacity of 192.78 mg·g-1 and 99.46 mg·g-1 for Pb(Ⅱ) and Cd(Ⅱ) respectively at pH 5.					
35998726	1	57	theme	aqueous	404:410	arg1	solutions					412:420	aqueous solutions	404:420	aqueous solutions	404:420	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	2	58	theme	FTIR	465:468	arg1	techniques					498:507	FTIR, XRD, SEM, BET, TGA and XPS techniques	465:507	FTIR, XRD, SEM, BET, TGA and XPS techniques	465:507	The CS-PEI-nGT was characterized by using FTIR, XRD, SEM, BET, TGA and XPS techniques.					
35998726	1	59	dep	treated	263:269	arg1	tailings					245:252	gold tailings	240:252	gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions	240:420	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	1	59	dep	treated	263:269	arg1	particle					254:261	particle	254:261	particle	254:261	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	7	60	theme	ion	1434:1436	arg1	exchange					1438:1445	ion exchange	1434:1445	ion exchange	1434:1445	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	4	61	theme	adsorption	727:736	arg1	model					738:742	pseudo-second-order kinetic adsorption model	699:742	pseudo-second-order kinetic adsorption model	699:742	The adsorption kinetics was described well by pseudo-second-order kinetic adsorption model, and suggested that chemisorption as the rate-controlling step for adsorption of Pb(Ⅱ) and Cd(Ⅱ).					
35998726	1	62	theme	gold	240:243	arg1	tailings					245:252	gold tailings	240:252	gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions	240:420	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	1	62	theme	gold	240:243	arg1	particle					254:261	particle	254:261	particle	254:261	In this work, a novel amino-modified chitosan/tailings composite (CS-PEI-nGT) was successfully synthesized from gold tailings particle treated by ball milling (nGT), chitosan (CS) and polyethyleneimine (PEI) as raw materials, for Lead (Pb(Ⅱ)) and Cadmium (Cd(Ⅱ)) removal from aqueous solutions.					
35998726	7	63	theme	electrostatic	1403:1415	arg1	interactions					1417:1428	electrostatic interactions	1403:1428	electrostatic interactions	1403:1428	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	8	64	theme	toxic	1542:1546	arg1	ions					1555:1558	toxic metals ions	1542:1558	toxic metals ions	1542:1558	Our work presents a promising strategy for tailings recycling and highly efficient removal of toxic metals ions from wastewater.					
35998726	0	65	theme	efficient	73:81	arg1	removal					83:89	selective and highly efficient removal	52:89	selective and highly efficient removal of lead and cadmium from wastewater	52:125	Amino-modified chitosan/gold tailings composite for selective and highly efficient removal of lead and cadmium from wastewater.					
35998726	7	66	from	groups	1258:1263	arg1	surface					1322:1328	composite surface	1312:1328	composite surface	1312:1328	The mechanism of the adsorption was investigated and the results revealed that amino (-NH2), silicon oxide groups (Si-O) and hydroxyl (-OH) functional groups on composite surface were accountable for metals adsorption, suggesting surface complexation, electrostatic interactions and ion exchange.					
35998726	8	67	theme	tailings	1491:1498	arg1	recycling					1500:1508	tailings recycling	1491:1508	tailings recycling	1491:1508	Our work presents a promising strategy for tailings recycling and highly efficient removal of toxic metals ions from wastewater.					
37023589	7	0	theme	M2	1515:1516	arg1	macrophage					1518:1527	M2 macrophage	1515:1527	M2 macrophage	1515:1527	Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair.					
37023589	8	1	theme	novel	1658:1662	arg1	therapy					1690:1696	the novel three-dimensional PDT/PTT therapy	1654:1696	the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study	1654:1751	In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.					
37023589	8	2	theme	clinical	1905:1912	arg1	strategies					1914:1923	clinical strategies	1905:1923	clinical strategies for phototherapy	1905:1940	In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.					
37023589	7	3	theme	macrophage	1501:1510	arg1	polarization					1482:1493	the polarization	1478:1493	the polarization of M1 macrophage to M2 macrophage	1478:1527	Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair.					
37023589	7	4	theme	lower	1456:1460	arg1	region					1462:1467	lower region	1456:1467	lower region	1456:1467	Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair.					
37023589	8	5	theme	PDT/PTT	1682:1688	arg1	therapy					1690:1696	the novel three-dimensional PDT/PTT therapy	1654:1696	the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study	1654:1751	In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.					
37023589	3	6	theme	autophagy	736:744	arg1	promotion					746:754	macrophage autophagy promotion	725:754	macrophage autophagy promotion	725:754	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	8	7	theme	strategies	1914:1923	arg1	development					1890:1900	the development	1886:1900	the development of clinical strategies for phototherapy	1886:1940	In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.					
37023589	7	8	dep	autophagy	1543:1551	arg1	promoting					1615:1623	promoting	1615:1623	promoting wound repair	1615:1636	Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair.					
37023589	7	8	dep	autophagy	1543:1551	arg1	regulating					1572:1581	regulating	1572:1581	regulating the immune microenvironment	1572:1609	Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair.					
37023589	4	9	theme	melanin	884:890	arg1	polymer					892:898	natural melanin polymer	876:898	natural melanin polymer	876:898	MGC NP is a composite polymer material composed of natural melanin polymer, oligopeptide and carbon-based material, which showed excellent biological safety.					
37023589	3	10	theme	wound	777:781	arg1	space					783:787	three-dimensional wound space	759:787	three-dimensional wound space	759:787	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	8	11	theme	dual	1787:1790	arg1	pathways					1792:1799	dual pathways	1787:1799	dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy	1787:1940	In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.					
37023589	4	12	theme	excellent	954:962	arg1	safety					975:980	excellent biological safety	954:980	excellent biological safety	954:980	MGC NP is a composite polymer material composed of natural melanin polymer, oligopeptide and carbon-based material, which showed excellent biological safety.					
37023589	3	13	from	promotion	746:754	arg1	space					783:787	three-dimensional wound space	759:787	three-dimensional wound space	759:787	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	5	14	from	hydrogel	1243:1250	arg1	regions					1301:1307	different regions	1291:1307	different regions	1291:1307	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	5	15	theme	upper	1101:1105	arg1	site					1113:1116	the upper wound site	1097:1116	the upper wound site	1097:1116	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	1	16	theme	photothermal	219:230	arg1	PTT					241:243	PTT	241:243	PTT	241:243	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	1	16	theme	photothermal	219:230	arg1	therapy					232:238	photothermal therapy	219:238	photothermal therapy (PTT)	219:244	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	3	17	theme	stress	808:813	arg1	response					815:822	stress response	808:822	stress response	808:822	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	3	18	theme	hydrogel	589:596	arg1	melanin-glycine-C60					598:616	a three-dimension chitosan hydrogel melanin-glycine-C60	562:616	a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs)	562:640	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	3	19	from	activation	710:719	arg1	space					783:787	three-dimensional wound space	759:787	three-dimensional wound space	759:787	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	5	20	theme	ROS/heat	1073:1080	arg1	environment					1082:1092	a high ROS/heat environment	1066:1092	a high ROS/heat environment at the upper wound site	1066:1116	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	3	21	theme	three-dimension	564:578	arg1	melanin-glycine-C60					598:616	a three-dimension chitosan hydrogel melanin-glycine-C60	562:616	a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs)	562:640	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	3	22	theme	MGC	633:635	arg1	nanoparticles					618:630	a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles	562:630	a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs)	562:640	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	3	22	theme	MGC	633:635	arg1	NPs					637:639	MGC NPs	633:639	MGC NPs	633:639	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	8	23	theme	stress	1834:1839	arg1	response					1841:1848	wound stress response	1828:1848	wound stress response	1828:1848	In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.					
37023589	2	24	theme	normal	493:498	arg1	tissues					500:506	normal tissues	493:506	normal tissues	493:506	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	0	25	theme	wound	78:82	arg1	healing					84:90	wound healing	78:90	wound healing	78:90	Three-dimension chitosan hydrogel loading melanin composite nanoparticles for wound healing by anti-bacteria, immune activation and macrophage autophagy promotion.					
37023589	7	26	from	PDT/PTT	1445:1451	arg1	region					1462:1467	lower region	1456:1467	lower region	1456:1467	Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair.					
37023589	1	27	theme	drug-resistant	341:354	arg1	strains					356:362	drug-resistant strains	341:362	drug-resistant strains	341:362	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	1	28	theme	photodynamic	188:199	arg1	PDT					210:212	PDT	210:212	PDT	210:212	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	1	28	theme	photodynamic	188:199	arg1	therapy					201:207	Combined photodynamic therapy	179:207	Combined photodynamic therapy (PDT)	179:213	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	5	29	theme	different	1291:1299	arg1	regions					1301:1307	different regions	1291:1307	different regions	1291:1307	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	8	30	theme	great	1863:1867	arg1	significance					1869:1880	great significance	1863:1880	great significance	1863:1880	In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.					
37023589	5	31	theme	ROS/heat	1128:1135	arg1	environment					1137:1147	a low ROS/heat environment	1122:1147	a low ROS/heat environment at the lower region adjacent to the wound tissue	1122:1196	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	0	32	theme	Three-dimension	0:14	arg1	composite					50:58	Three-dimension chitosan hydrogel loading melanin composite	0:58	Three-dimension chitosan hydrogel loading melanin composite	0:58	Three-dimension chitosan hydrogel loading melanin composite nanoparticles for wound healing by anti-bacteria, immune activation and macrophage autophagy promotion.					
37023589	1	33	theme	promising	273:281	arg1	strategy					283:290	the most promising strategy	264:290	the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing	264:383	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	2	34	theme	species	426:432	arg1	temperature					449:459	high temperature	444:459	high temperature	444:459	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	2	34	theme	species	426:432	arg1	amount					400:405	high amount	395:405	high amount of reactive oxygen species (ROS)	395:438	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	2	34	theme	species	426:432	arg1	ROS					435:437	ROS	435:437	ROS	435:437	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	2	34	theme	species	426:432	arg1	species					426:432	reactive oxygen species	410:432	reactive oxygen species (ROS)	410:438	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	0	35	theme	hydrogel	25:32	arg1	composite					50:58	Three-dimension chitosan hydrogel loading melanin composite	0:58	Three-dimension chitosan hydrogel loading melanin composite	0:58	Three-dimension chitosan hydrogel loading melanin composite nanoparticles for wound healing by anti-bacteria, immune activation and macrophage autophagy promotion.					
37023589	7	36	theme	macrophages	1559:1569	arg1	autophagy					1543:1551	autophagy	1543:1551	autophagy	1543:1551	Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair.					
37023589	2	37	theme	severe	467:472	arg1	response					481:488	severe stress response	467:488	severe stress response to normal tissues	467:506	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	4	38	theme	composite	837:845	arg1	MGC					825:827	MGC NP	825:830	MGC NP	825:830	MGC NP is a composite polymer material composed of natural melanin polymer, oligopeptide and carbon-based material, which showed excellent biological safety.					
37023589	4	38	theme	composite	837:845	arg1	material					855:862	a composite polymer material	835:862	a composite polymer material	835:862	MGC NP is a composite polymer material composed of natural melanin polymer, oligopeptide and carbon-based material, which showed excellent biological safety.					
37023589	2	39	theme	reactive	410:417	arg1	ROS					435:437	ROS	435:437	ROS	435:437	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	2	39	theme	reactive	410:417	arg1	species					426:432	reactive oxygen species	410:432	reactive oxygen species (ROS)	410:438	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	0	40	theme	melanin	42:48	arg1	composite					50:58	Three-dimension chitosan hydrogel loading melanin composite	0:58	Three-dimension chitosan hydrogel loading melanin composite	0:58	Three-dimension chitosan hydrogel loading melanin composite nanoparticles for wound healing by anti-bacteria, immune activation and macrophage autophagy promotion.					
37023589	5	41	theme	adjacent	1169:1176	arg1	region					1162:1167	the lower region	1152:1167	the lower region adjacent to the wound tissue	1152:1196	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	5	42	from	region	1162:1167	arg1	environment					1137:1147	a low ROS/heat environment	1122:1147	a low ROS/heat environment at the lower region adjacent to the wound tissue	1122:1196	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	5	42	from	region	1162:1167	arg1	environment					1082:1092	a high ROS/heat environment	1066:1092	a high ROS/heat environment at the upper wound site	1066:1116	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	5	43	with	hydrogel	1243:1250	arg1	PDT					1265:1267	precise PDT	1257:1267	precise PDT	1257:1267	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	5	43	with	hydrogel	1243:1250	arg1	efficiency					1277:1286	PTT efficiency	1273:1286	PTT efficiency	1273:1286	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	6	44	theme	microbial	1419:1427	arg1	infection					1429:1437	microbial infection	1419:1437	microbial infection	1419:1437	Highly effective PDT/PTT was used to kill microorganisms in upper region, thus providing a barrier to reduce microbial infection.					
37023589	5	45	theme	high	1068:1071	arg1	environment					1082:1092	a high ROS/heat environment	1066:1092	a high ROS/heat environment at the upper wound site	1066:1116	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	3	46	theme	anti-bacterial	678:691	arg1	activity					693:700	realized effective anti-bacterial activity	659:700	realized effective anti-bacterial activity	659:700	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	5	47	theme	C60	1036:1038	arg1	content					1057:1063	C60 and nanoparticle content	1036:1063	content	1057:1063	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	3	48	theme	realized	659:666	arg1	activity					693:700	realized effective anti-bacterial activity	659:700	realized effective anti-bacterial activity	659:700	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	5	49	theme	nanoparticle	1044:1055	arg1	content					1057:1063	C60 and nanoparticle content	1036:1063	content	1057:1063	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	6	50	theme	effective	1317:1325	arg1	PDT/PTT					1327:1333	Highly effective PDT/PTT	1310:1333	Highly effective PDT/PTT	1310:1333	Highly effective PDT/PTT was used to kill microorganisms in upper region, thus providing a barrier to reduce microbial infection.					
37023589	2	51	theme	wound	539:543	arg1	healing					545:551	wound healing	539:551	wound healing	539:551	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	7	52	theme	M1	1498:1499	arg1	macrophage					1501:1510	M1 macrophage	1498:1510	M1 macrophage	1498:1510	Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair.					
37023589	5	53	theme	three-dimension	1227:1241	arg1	hydrogel					1243:1250	a three-dimension hydrogel	1225:1250	a three-dimension hydrogel with precise PDT and PTT efficiency in different regions	1225:1307	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	8	54	theme	three-dimensional	1664:1680	arg1	therapy					1690:1696	the novel three-dimensional PDT/PTT therapy	1654:1696	the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study	1654:1751	In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.					
37023589	1	55	theme	therapy	201:207	arg1	Application					164:174	Application	164:174	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT)	164:244	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	2	56	theme	high	444:447	arg1	temperature					449:459	high temperature	444:459	high temperature	444:459	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	3	57	theme	macrophage	725:734	arg1	promotion					746:754	macrophage autophagy promotion	725:754	macrophage autophagy promotion	725:754	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	8	58	theme	wound	1765:1769	arg1	healing					1771:1777	wound healing	1765:1777	wound healing	1765:1777	In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.					
37023589	5	59	theme	lower	1156:1160	arg1	region					1162:1167	the lower region	1152:1167	the lower region adjacent to the wound tissue	1152:1196	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	4	60	theme	natural	876:882	arg1	polymer					892:898	natural melanin polymer	876:898	natural melanin polymer	876:898	MGC NP is a composite polymer material composed of natural melanin polymer, oligopeptide and carbon-based material, which showed excellent biological safety.					
37023589	7	61	theme	Mild	1440:1443	arg1	PDT/PTT					1445:1451	Mild PDT/PTT	1440:1451	Mild PDT/PTT in lower region	1440:1467	Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair.					
37023589	6	62	theme	upper	1370:1374	arg1	region					1376:1381	upper region	1370:1381	upper region	1370:1381	Highly effective PDT/PTT was used to kill microorganisms in upper region, thus providing a barrier to reduce microbial infection.					
37023589	6	63	used	used	1339:1342	arg2	PDT/PTT					1327:1333	Highly effective PDT/PTT	1310:1333	Highly effective PDT/PTT	1310:1333	Highly effective PDT/PTT was used to kill microorganisms in upper region, thus providing a barrier to reduce microbial infection.					
37023589	3	64	from	activity	693:700	arg1	space					783:787	three-dimensional wound space	759:787	three-dimensional wound space	759:787	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	3	65	theme	three-dimensional	759:775	arg1	space					783:787	three-dimensional wound space	759:787	three-dimensional wound space	759:787	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	5	66	theme	wound	1107:1111	arg1	site					1113:1116	the upper wound site	1097:1116	the upper wound site	1097:1116	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	3	67	theme	chitosan	580:587	arg1	melanin-glycine-C60					598:616	a three-dimension chitosan hydrogel melanin-glycine-C60	562:616	a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs)	562:640	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	1	68	theme	therapy	232:238	arg1	Application					164:174	Application	164:174	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT)	164:244	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	4	69	theme	biological	964:973	arg1	safety					975:980	excellent biological safety	954:980	excellent biological safety	954:980	MGC NP is a composite polymer material composed of natural melanin polymer, oligopeptide and carbon-based material, which showed excellent biological safety.					
37023589	5	70	from	site	1113:1116	arg1	environment					1137:1147	a low ROS/heat environment	1122:1147	a low ROS/heat environment at the lower region adjacent to the wound tissue	1122:1196	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	5	70	from	site	1113:1116	arg1	environment					1082:1092	a high ROS/heat environment	1066:1092	a high ROS/heat environment at the upper wound site	1066:1116	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	0	71	theme	autophagy	143:151	arg1	promotion					153:161	macrophage autophagy promotion	132:161	macrophage autophagy promotion	132:161	Three-dimension chitosan hydrogel loading melanin composite nanoparticles for wound healing by anti-bacteria, immune activation and macrophage autophagy promotion.					
37023589	4	72	theme	carbon-based	918:929	arg1	material					931:938	carbon-based material	918:938	carbon-based material	918:938	MGC NP is a composite polymer material composed of natural melanin polymer, oligopeptide and carbon-based material, which showed excellent biological safety.					
37023589	7	73	theme	wound	1625:1629	arg1	repair					1631:1636	wound repair	1625:1636	wound repair	1625:1636	Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair.					
37023589	2	74	theme	healing	545:551	arg1	risks					530:534	potential risks	520:534	potential risks of wound healing	520:551	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	3	75	theme	melanin-glycine-C60	598:616	arg1	nanoparticles					618:630	a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles	562:630	a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs)	562:640	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	3	75	theme	melanin-glycine-C60	598:616	arg1	NPs					637:639	MGC NPs	633:639	MGC NPs	633:639	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	8	76	theme	wound	1828:1832	arg1	response					1841:1848	wound stress response	1828:1848	wound stress response	1828:1848	In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.					
37023589	5	77	theme	PTT	1273:1275	arg1	efficiency					1277:1286	PTT efficiency	1273:1286	PTT efficiency	1273:1286	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	1	78	theme	Combined	179:186	arg1	PDT					210:212	PDT	210:212	PDT	210:212	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	1	78	theme	Combined	179:186	arg1	therapy					201:207	Combined photodynamic therapy	179:207	Combined photodynamic therapy (PDT)	179:213	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	7	79	theme	immune	1587:1592	arg1	microenvironment					1594:1609	the immune microenvironment	1583:1609	the immune microenvironment	1583:1609	Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair.					
37023589	2	80	theme	stress	474:479	arg1	response					481:488	severe stress response	467:488	severe stress response to normal tissues	467:506	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	0	81	theme	immune	110:115	arg1	activation					117:126	immune activation	110:126	immune activation	110:126	Three-dimension chitosan hydrogel loading melanin composite nanoparticles for wound healing by anti-bacteria, immune activation and macrophage autophagy promotion.					
37023589	1	82	theme	strains	356:362	arg1	epidemic					329:336	epidemic	329:336	epidemic	329:336	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	5	83	theme	peptide	1001:1007	arg1	length					1009:1014	the peptide length	997:1014	the peptide length between melanin and C60 and nanoparticle content	997:1063	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	1	84	theme	wound	371:375	arg1	healing					377:383	wound healing	371:383	wound healing	371:383	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	2	85	theme	high	395:398	arg1	amount					400:405	high amount	395:405	high amount of reactive oxygen species (ROS)	395:438	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	2	85	theme	high	395:398	arg1	ROS					435:437	ROS	435:437	ROS	435:437	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	2	85	theme	high	395:398	arg1	species					426:432	reactive oxygen species	410:432	reactive oxygen species (ROS)	410:438	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	0	86	theme	chitosan	16:23	arg1	composite					50:58	Three-dimension chitosan hydrogel loading melanin composite	0:58	Three-dimension chitosan hydrogel loading melanin composite	0:58	Three-dimension chitosan hydrogel loading melanin composite nanoparticles for wound healing by anti-bacteria, immune activation and macrophage autophagy promotion.					
37023589	5	87	theme	low	1124:1126	arg1	environment					1137:1147	a low ROS/heat environment	1122:1147	a low ROS/heat environment at the lower region adjacent to the wound tissue	1122:1196	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	0	88	theme	loading	34:40	arg1	composite					50:58	Three-dimension chitosan hydrogel loading melanin composite	0:58	Three-dimension chitosan hydrogel loading melanin composite	0:58	Three-dimension chitosan hydrogel loading melanin composite nanoparticles for wound healing by anti-bacteria, immune activation and macrophage autophagy promotion.					
37023589	1	89	theme	strategy	283:290	arg1	strategy					283:290	the most promising strategy	264:290	the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing	264:383	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	1	89	theme	strategy	283:290	arg1	one					257:259	one	257:259	one	257:259	Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing.					
37023589	2	90	theme	oxygen	419:424	arg1	ROS					435:437	ROS	435:437	ROS	435:437	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	2	90	theme	oxygen	419:424	arg1	species					426:432	reactive oxygen species	410:432	reactive oxygen species (ROS)	410:438	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	7	91	theme	M2	1556:1557	arg1	macrophages					1559:1569	M2 macrophages	1556:1569	M2 macrophages	1556:1569	Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair.					
37023589	2	92	theme	potential	520:528	arg1	risks					530:534	potential risks	520:534	potential risks of wound healing	520:551	However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing.					
37023589	3	93	theme	effective	668:676	arg1	activity					693:700	realized effective anti-bacterial activity	659:700	realized effective anti-bacterial activity	659:700	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
37023589	4	94	theme	polymer	847:853	arg1	MGC					825:827	MGC NP	825:830	MGC NP	825:830	MGC NP is a composite polymer material composed of natural melanin polymer, oligopeptide and carbon-based material, which showed excellent biological safety.					
37023589	4	94	theme	polymer	847:853	arg1	material					855:862	a composite polymer material	835:862	a composite polymer material	835:862	MGC NP is a composite polymer material composed of natural melanin polymer, oligopeptide and carbon-based material, which showed excellent biological safety.					
37023589	5	95	theme	wound	1185:1189	arg1	tissue					1191:1196	the wound tissue	1181:1196	the wound tissue	1181:1196	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	8	96	from	pathways	1792:1799	arg1	premise					1808:1814	the premise	1804:1814	the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy	1804:1940	In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.					
37023589	8	97	theme	natural	1707:1713	arg1	macromolecules					1715:1728	natural macromolecules	1707:1728	natural macromolecules proposed in this study	1707:1751	In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.					
37023589	5	98	theme	precise	1257:1263	arg1	PDT					1265:1267	precise PDT	1257:1267	precise PDT	1257:1267	By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions.					
37023589	0	99	theme	macrophage	132:141	arg1	promotion					153:161	macrophage autophagy promotion	132:161	macrophage autophagy promotion	132:161	Three-dimension chitosan hydrogel loading melanin composite nanoparticles for wound healing by anti-bacteria, immune activation and macrophage autophagy promotion.					
37023589	3	100	theme	immune	703:708	arg1	activation					710:719	immune activation	703:719	immune activation	703:719	Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response.					
35640852	0	0	theme	propolis	97:104	arg1	extract					106:112	polyphenol propolis extract	86:112	polyphenol propolis extract envisaging food packaging applications	86:151	Production of chitosan-based biodegradable active films using bio-waste enriched with polyphenol propolis extract envisaging food packaging applications.					
35640852	6	1	theme	tensile	1209:1215	arg1	films					1153:1157	the films thermal stability and mechanical properties e.g.	1149:1206	the films thermal stability and mechanical properties e.g.	1149:1206	The findings also showed that addition of PS enhanced the films thermal stability and mechanical properties e.g., tensile modulus, yield strength, and stress at break.					
35640852	6	1	theme	tensile	1209:1215	arg1	modulus					1217:1223	tensile modulus	1209:1223	tensile modulus	1209:1223	The findings also showed that addition of PS enhanced the films thermal stability and mechanical properties e.g., tensile modulus, yield strength, and stress at break.					
35640852	4	2	theme	increasing	694:703	arg1	addition					713:720	increasing extract addition	694:720	increasing extract addition to the chitosan	694:736	With increasing extract addition to the chitosan, the thickness of the films increased from 61.7 to 71.7 μm, causing a reduction in the light transmission rate, along with a greenish colour shift.					
35640852	4	3	theme	greenish	863:870	arg1	shift					879:883	a greenish colour shift	861:883	a greenish colour shift	861:883	With increasing extract addition to the chitosan, the thickness of the films increased from 61.7 to 71.7 μm, causing a reduction in the light transmission rate, along with a greenish colour shift.					
35640852	8	4	theme	synthetic	1418:1426	arg1	plastics					1428:1435	petroleum-based synthetic plastics	1402:1435	petroleum-based synthetic plastics	1402:1435	Overall, CS-based composite films seem a promising green alternative to petroleum-based synthetic plastics allowing to extend the shelf life of food products due to their eco-friendly nature.					
35640852	0	5	theme	polyphenol	86:95	arg1	extract					106:112	polyphenol propolis extract	86:112	polyphenol propolis extract envisaging food packaging applications	86:151	Production of chitosan-based biodegradable active films using bio-waste enriched with polyphenol propolis extract envisaging food packaging applications.					
35640852	8	6	theme	products	1479:1486	arg1	life					1466:1469	the shelf life	1456:1469	the shelf life of food products	1456:1486	Overall, CS-based composite films seem a promising green alternative to petroleum-based synthetic plastics allowing to extend the shelf life of food products due to their eco-friendly nature.					
35640852	1	7	theme	human	295:299	arg1	health					301:306	human health	295:306	human health	295:306	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	8	8	theme	food	1474:1477	arg1	products					1479:1486	food products	1474:1486	food products	1474:1486	Overall, CS-based composite films seem a promising green alternative to petroleum-based synthetic plastics allowing to extend the shelf life of food products due to their eco-friendly nature.					
35640852	8	9	theme	green	1381:1385	arg1	alternative					1387:1397	a promising green alternative	1369:1397	a promising green alternative to petroleum-based synthetic plastics	1369:1435	Overall, CS-based composite films seem a promising green alternative to petroleum-based synthetic plastics allowing to extend the shelf life of food products due to their eco-friendly nature.					
35640852	2	10	theme	packaging	571:579	arg1	applications					581:592	food packaging applications	566:592	food packaging applications	566:592	This study aimed to develop active films based on chitosan (CS), produced from waste crayfish (Procambarus clarkii) shells enriched with bioactive extract (5-20%) of propolis (PS) and to characterize its properties, envisaging food packaging applications.					
35640852	0	11	theme	packaging	130:138	arg1	applications					140:151	food packaging applications	125:151	food packaging applications	125:151	Production of chitosan-based biodegradable active films using bio-waste enriched with polyphenol propolis extract envisaging food packaging applications.					
35640852	5	12	theme	scanning	1053:1060	arg1	microscopy					1071:1080	scanning electron microscopy	1053:1080	the scanning electron microscopy micrographs	1049:1092	The interactions between PS extract and CS was confirmed by infrared spectroscopy, at the same time that the microstructural integrity of the films was checked on the scanning electron microscopy micrographs.					
35640852	6	13	theme	yield	1226:1230	arg1	films					1153:1157	the films thermal stability and mechanical properties e.g.	1149:1206	the films thermal stability and mechanical properties e.g.	1149:1206	The findings also showed that addition of PS enhanced the films thermal stability and mechanical properties e.g., tensile modulus, yield strength, and stress at break.					
35640852	6	13	theme	yield	1226:1230	arg1	strength					1232:1239	yield strength	1226:1239	yield strength	1226:1239	The findings also showed that addition of PS enhanced the films thermal stability and mechanical properties e.g., tensile modulus, yield strength, and stress at break.					
35640852	6	14	theme	PS	1137:1138	arg1	addition					1125:1132	addition	1125:1132	addition of PS	1125:1138	The findings also showed that addition of PS enhanced the films thermal stability and mechanical properties e.g., tensile modulus, yield strength, and stress at break.					
35640852	7	15	theme	antioxidant	1288:1298	arg1	activities					1318:1327	the antioxidant and antimicrobial activities	1284:1327	the antioxidant and antimicrobial activities	1284:1327	Besides, it improved the antioxidant and antimicrobial activities.					
35640852	4	16	dep	71.7 μm	789:795	arg1	to					786:787	to	786:787	to	786:787	With increasing extract addition to the chitosan, the thickness of the films increased from 61.7 to 71.7 μm, causing a reduction in the light transmission rate, along with a greenish colour shift.					
35640852	0	17	theme	food	125:128	arg1	applications					140:151	food packaging applications	125:151	food packaging applications	125:151	Production of chitosan-based biodegradable active films using bio-waste enriched with polyphenol propolis extract envisaging food packaging applications.					
35640852	5	18	theme	electron	1062:1069	arg1	microscopy					1071:1080	scanning electron microscopy	1053:1080	the scanning electron microscopy micrographs	1049:1092	The interactions between PS extract and CS was confirmed by infrared spectroscopy, at the same time that the microstructural integrity of the films was checked on the scanning electron microscopy micrographs.					
35640852	4	19	theme	films	760:764	arg1	thickness					743:751	the thickness	739:751	the thickness of the films	739:764	With increasing extract addition to the chitosan, the thickness of the films increased from 61.7 to 71.7 μm, causing a reduction in the light transmission rate, along with a greenish colour shift.					
35640852	1	20	theme	promising	203:211	arg1	films					186:190	Developing biodegradable active films	154:190	Developing biodegradable active films	154:190	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	1	20	theme	promising	203:211	arg1	approach					219:226	a promising green approach	201:226	a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization	201:336	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	5	21	theme	microscopy	1071:1080	arg1	micrographs					1082:1092	the scanning electron microscopy micrographs	1049:1092	the scanning electron microscopy micrographs	1049:1092	The interactions between PS extract and CS was confirmed by infrared spectroscopy, at the same time that the microstructural integrity of the films was checked on the scanning electron microscopy micrographs.					
35640852	4	22	theme	transmission	831:842	arg1	rate					844:847	the light transmission rate	821:847	the light transmission rate	821:847	With increasing extract addition to the chitosan, the thickness of the films increased from 61.7 to 71.7 μm, causing a reduction in the light transmission rate, along with a greenish colour shift.					
35640852	5	23	theme	PS	911:912	arg1	extract					914:920	PS extract	911:920	PS extract	911:920	The interactions between PS extract and CS was confirmed by infrared spectroscopy, at the same time that the microstructural integrity of the films was checked on the scanning electron microscopy micrographs.					
35640852	1	24	theme	green	213:217	arg1	films					186:190	Developing biodegradable active films	154:190	Developing biodegradable active films	154:190	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	1	24	theme	green	213:217	arg1	approach					219:226	a promising green approach	201:226	a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization	201:336	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	6	25	theme	thermal	1159:1165	arg1	stability					1167:1175	thermal stability	1159:1175	thermal stability	1159:1175	The findings also showed that addition of PS enhanced the films thermal stability and mechanical properties e.g., tensile modulus, yield strength, and stress at break.					
35640852	4	26	theme	light	825:829	arg1	rate					844:847	the light transmission rate	821:847	the light transmission rate	821:847	With increasing extract addition to the chitosan, the thickness of the films increased from 61.7 to 71.7 μm, causing a reduction in the light transmission rate, along with a greenish colour shift.					
35640852	7	27	theme	antimicrobial	1304:1316	arg1	activities					1318:1327	the antioxidant and antimicrobial activities	1284:1327	the antioxidant and antimicrobial activities	1284:1327	Besides, it improved the antioxidant and antimicrobial activities.					
35640852	8	28	theme	petroleum-based	1402:1416	arg1	plastics					1428:1435	petroleum-based synthetic plastics	1402:1435	petroleum-based synthetic plastics	1402:1435	Overall, CS-based composite films seem a promising green alternative to petroleum-based synthetic plastics allowing to extend the shelf life of food products due to their eco-friendly nature.					
35640852	0	29	theme	biodegradable	29:41	arg1	films					50:54	chitosan-based biodegradable active films	14:54	chitosan-based biodegradable active films using bio-waste	14:70	Production of chitosan-based biodegradable active films using bio-waste enriched with polyphenol propolis extract envisaging food packaging applications.					
35640852	1	30	theme	plastic	318:324	arg1	utilization					326:336	plastic utilization	318:336	plastic utilization	318:336	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	8	31	theme	CS-based	1339:1346	arg1	films					1358:1362	CS-based composite films	1339:1362	CS-based composite films	1339:1362	Overall, CS-based composite films seem a promising green alternative to petroleum-based synthetic plastics allowing to extend the shelf life of food products due to their eco-friendly nature.					
35640852	2	32	theme	Procambarus	434:444	arg1	clarkii					446:452	waste crayfish (Procambarus clarkii)	418:453	waste crayfish (Procambarus clarkii)	418:453	This study aimed to develop active films based on chitosan (CS), produced from waste crayfish (Procambarus clarkii) shells enriched with bioactive extract (5-20%) of propolis (PS) and to characterize its properties, envisaging food packaging applications.					
35640852	0	33	theme	chitosan-based	14:27	arg1	films					50:54	chitosan-based biodegradable active films	14:54	chitosan-based biodegradable active films using bio-waste	14:70	Production of chitosan-based biodegradable active films using bio-waste enriched with polyphenol propolis extract envisaging food packaging applications.					
35640852	5	34	theme	films	1028:1032	arg1	integrity					1011:1019	the microstructural integrity	991:1019	the microstructural integrity of the films	991:1032	The interactions between PS extract and CS was confirmed by infrared spectroscopy, at the same time that the microstructural integrity of the films was checked on the scanning electron microscopy micrographs.					
35640852	0	35	theme	films	50:54	arg1	Production					0:9	Production	0:9	Production of chitosan-based biodegradable active films using bio-waste	0:70	Production of chitosan-based biodegradable active films using bio-waste enriched with polyphenol propolis extract envisaging food packaging applications.					
35640852	6	36	dep	films	1153:1157	arg1	stability					1167:1175	thermal stability	1159:1175	thermal stability	1159:1175	The findings also showed that addition of PS enhanced the films thermal stability and mechanical properties e.g., tensile modulus, yield strength, and stress at break.					
35640852	6	36	dep	films	1153:1157	arg1	properties					1192:1201	mechanical properties	1181:1201	mechanical properties e.g.	1181:1206	The findings also showed that addition of PS enhanced the films thermal stability and mechanical properties e.g., tensile modulus, yield strength, and stress at break.					
35640852	1	37	dep	pollution	281:289	arg1	the					263:265	the	263:265	the	263:265	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	5	38	theme	same	976:979	arg1	time					981:984	the same time that the microstructural integrity of the films was checked on the scanning electron microscopy micrographs	972:1092	the same time that the microstructural integrity of the films was checked on the scanning electron microscopy micrographs	972:1092	The interactions between PS extract and CS was confirmed by infrared spectroscopy, at the same time that the microstructural integrity of the films was checked on the scanning electron microscopy micrographs.					
35640852	2	39	theme	crayfish	424:431	arg1	clarkii					446:452	waste crayfish (Procambarus clarkii)	418:453	waste crayfish (Procambarus clarkii)	418:453	This study aimed to develop active films based on chitosan (CS), produced from waste crayfish (Procambarus clarkii) shells enriched with bioactive extract (5-20%) of propolis (PS) and to characterize its properties, envisaging food packaging applications.					
35640852	0	40	theme	active	43:48	arg1	films					50:54	chitosan-based biodegradable active films	14:54	chitosan-based biodegradable active films using bio-waste	14:70	Production of chitosan-based biodegradable active films using bio-waste enriched with polyphenol propolis extract envisaging food packaging applications.					
35640852	4	41	dep	along	850:854	arg1	with					856:859	with	856:859	with	856:859	With increasing extract addition to the chitosan, the thickness of the films increased from 61.7 to 71.7 μm, causing a reduction in the light transmission rate, along with a greenish colour shift.					
35640852	3	42	theme	extract	629:635	arg1	profile					615:621	The chromatographic profile	595:621	The chromatographic profile of PS extract	595:635	The chromatographic profile of PS extract confirmed its richness, with 41 phenolic compounds.					
35640852	8	43	theme	composite	1348:1356	arg1	films					1358:1362	CS-based composite films	1339:1362	CS-based composite films	1339:1362	Overall, CS-based composite films seem a promising green alternative to petroleum-based synthetic plastics allowing to extend the shelf life of food products due to their eco-friendly nature.					
35640852	4	44	from	reduction	808:816	arg1	rate					844:847	the light transmission rate	821:847	the light transmission rate	821:847	With increasing extract addition to the chitosan, the thickness of the films increased from 61.7 to 71.7 μm, causing a reduction in the light transmission rate, along with a greenish colour shift.					
35640852	2	45	theme	waste	418:422	arg1	clarkii					446:452	waste crayfish (Procambarus clarkii)	418:453	waste crayfish (Procambarus clarkii)	418:453	This study aimed to develop active films based on chitosan (CS), produced from waste crayfish (Procambarus clarkii) shells enriched with bioactive extract (5-20%) of propolis (PS) and to characterize its properties, envisaging food packaging applications.					
35640852	2	46	theme	bioactive	476:484	arg1	extract					486:492	bioactive extract	476:492	bioactive extract (5-20%) of propolis (PS)	476:517	This study aimed to develop active films based on chitosan (CS), produced from waste crayfish (Procambarus clarkii) shells enriched with bioactive extract (5-20%) of propolis (PS) and to characterize its properties, envisaging food packaging applications.					
35640852	2	46	theme	bioactive	476:484	arg1	%					499:499	5-20%	495:499	5-20%	495:499	This study aimed to develop active films based on chitosan (CS), produced from waste crayfish (Procambarus clarkii) shells enriched with bioactive extract (5-20%) of propolis (PS) and to characterize its properties, envisaging food packaging applications.					
35640852	2	47	theme	food	566:569	arg1	applications					581:592	food packaging applications	566:592	food packaging applications	566:592	This study aimed to develop active films based on chitosan (CS), produced from waste crayfish (Procambarus clarkii) shells enriched with bioactive extract (5-20%) of propolis (PS) and to characterize its properties, envisaging food packaging applications.					
35640852	8	48	theme	eco-friendly	1501:1512	arg1	nature					1514:1519	their eco-friendly nature	1495:1519	their eco-friendly nature	1495:1519	Overall, CS-based composite films seem a promising green alternative to petroleum-based synthetic plastics allowing to extend the shelf life of food products due to their eco-friendly nature.					
35640852	5	49	theme	microstructural	995:1009	arg1	integrity					1011:1019	the microstructural integrity	991:1019	the microstructural integrity of the films	991:1032	The interactions between PS extract and CS was confirmed by infrared spectroscopy, at the same time that the microstructural integrity of the films was checked on the scanning electron microscopy micrographs.					
35640852	6	50	theme	mechanical	1181:1190	arg1	properties					1192:1201	mechanical properties	1181:1201	mechanical properties e.g.	1181:1206	The findings also showed that addition of PS enhanced the films thermal stability and mechanical properties e.g., tensile modulus, yield strength, and stress at break.					
35640852	4	51	theme	extract	705:711	arg1	addition					713:720	increasing extract addition	694:720	increasing extract addition to the chitosan	694:736	With increasing extract addition to the chitosan, the thickness of the films increased from 61.7 to 71.7 μm, causing a reduction in the light transmission rate, along with a greenish colour shift.					
35640852	3	52	theme	phenolic	669:676	arg1	compounds					678:686	41 phenolic compounds	666:686	41 phenolic compounds	666:686	The chromatographic profile of PS extract confirmed its richness, with 41 phenolic compounds.					
35640852	1	53	dep	global	242:247	arg1	concerns					249:256	concerns	249:256	concerns	249:256	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	1	54	theme	Developing	154:163	arg1	films					186:190	Developing biodegradable active films	154:190	Developing biodegradable active films	154:190	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	1	54	theme	Developing	154:163	arg1	approach					219:226	a promising green approach	201:226	a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization	201:336	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	2	55	theme	propolis	505:512	arg1	extract					486:492	bioactive extract	476:492	bioactive extract (5-20%) of propolis (PS)	476:517	This study aimed to develop active films based on chitosan (CS), produced from waste crayfish (Procambarus clarkii) shells enriched with bioactive extract (5-20%) of propolis (PS) and to characterize its properties, envisaging food packaging applications.					
35640852	2	55	theme	propolis	505:512	arg1	%					499:499	5-20%	495:499	5-20%	495:499	This study aimed to develop active films based on chitosan (CS), produced from waste crayfish (Procambarus clarkii) shells enriched with bioactive extract (5-20%) of propolis (PS) and to characterize its properties, envisaging food packaging applications.					
35640852	4	56	theme	colour	872:877	arg1	shift					879:883	a greenish colour shift	861:883	a greenish colour shift	861:883	With increasing extract addition to the chitosan, the thickness of the films increased from 61.7 to 71.7 μm, causing a reduction in the light transmission rate, along with a greenish colour shift.					
35640852	8	57	theme	shelf	1460:1464	arg1	life					1466:1469	the shelf life	1456:1469	the shelf life of food products	1456:1486	Overall, CS-based composite films seem a promising green alternative to petroleum-based synthetic plastics allowing to extend the shelf life of food products due to their eco-friendly nature.					
35640852	3	58	theme	PS	626:627	arg1	extract					629:635	PS extract	626:635	PS extract	626:635	The chromatographic profile of PS extract confirmed its richness, with 41 phenolic compounds.					
35640852	1	59	theme	biodegradable	165:177	arg1	films					186:190	Developing biodegradable active films	154:190	Developing biodegradable active films	154:190	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	1	59	theme	biodegradable	165:177	arg1	approach					219:226	a promising green approach	201:226	a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization	201:336	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	2	60	theme	active	367:372	arg1	films					374:378	active films	367:378	active films based on chitosan (CS)	367:401	This study aimed to develop active films based on chitosan (CS), produced from waste crayfish (Procambarus clarkii) shells enriched with bioactive extract (5-20%) of propolis (PS) and to characterize its properties, envisaging food packaging applications.					
35640852	8	61	theme	promising	1371:1379	arg1	alternative					1387:1397	a promising green alternative	1369:1397	a promising green alternative to petroleum-based synthetic plastics	1369:1435	Overall, CS-based composite films seem a promising green alternative to petroleum-based synthetic plastics allowing to extend the shelf life of food products due to their eco-friendly nature.					
35640852	3	62	theme	chromatographic	599:613	arg1	profile					615:621	The chromatographic profile	595:621	The chromatographic profile of PS extract	595:635	The chromatographic profile of PS extract confirmed its richness, with 41 phenolic compounds.					
35640852	1	63	theme	active	179:184	arg1	films					186:190	Developing biodegradable active films	154:190	Developing biodegradable active films	154:190	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	1	63	theme	active	179:184	arg1	approach					219:226	a promising green approach	201:226	a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization	201:336	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	1	64	theme	environmental	267:279	arg1	pollution					281:289	environmental pollution	267:289	environmental pollution	267:289	Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization.					
35640852	5	65	theme	infrared	946:953	arg1	spectroscopy					955:966	infrared spectroscopy	946:966	infrared spectroscopy	946:966	The interactions between PS extract and CS was confirmed by infrared spectroscopy, at the same time that the microstructural integrity of the films was checked on the scanning electron microscopy micrographs.					
35640686	0	0	link	cross-linked	102:113	arg1	/chitosan					135:143	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan	87:143	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan	87:143	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan.					
35640686	2	1	theme	vinyl	361:365	arg1	nanocomposite					385:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite	327:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC)	327:419	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	2	1	theme	vinyl	361:365	arg1	NC					417:418	MWCNTs/CS-PVA/GA NC	400:418	MWCNTs/CS-PVA/GA NC	400:418	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	3	2	theme	NC	532:533	arg1	synthesis					519:527	The successful synthesis	504:527	The successful synthesis of NC	504:533	The successful synthesis of NC was supported by techniques of SEM, XRD, TGA, FTIR, and EDX.					
35640686	10	3	theme	promising	1833:1841	arg1	NC					1807:1808	the developed MWCNTs/CS-PVA/GA NC	1776:1808	the developed MWCNTs/CS-PVA/GA NC	1776:1808	All in all, the developed MWCNTs/CS-PVA/GA NC can be considered as a promising candidate in dealing with aqueous solutions' pollution with antibiotic.					
35640686	10	3	theme	promising	1833:1841	arg1	candidate					1843:1851	a promising candidate	1831:1851	a promising candidate in dealing with aqueous solutions' pollution with antibiotic	1831:1912	All in all, the developed MWCNTs/CS-PVA/GA NC can be considered as a promising candidate in dealing with aqueous solutions' pollution with antibiotic.					
35640686	3	4	theme	SEM	566:568	arg1	techniques					552:561	techniques	552:561	techniques of SEM, XRD, TGA, FTIR, and EDX	552:593	The successful synthesis of NC was supported by techniques of SEM, XRD, TGA, FTIR, and EDX.					
35640686	0	5	theme	alcohol	127:133	arg1	/chitosan					135:143	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan	87:143	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan	87:143	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan.					
35640686	5	6	theme	neural	952:957	arg1	network					959:965	general regression neural network	933:965	general regression neural network (GRNN)	933:972	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	5	6	theme	neural	952:957	arg1	GRNN					968:971	GRNN	968:971	GRNN	968:971	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	1	7	theme	novel	213:217	arg1	approaches					219:228	novel approaches	213:228	novel approaches	213:228	The presence of pharmaceuticals as the emerging contaminates needs novel approaches and new materials to be remediated.					
35640686	6	8	dep	efficiency	1161:1170	arg1	the					1157:1159	the	1157:1159	the	1157:1159	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	4	9	theme	prepared	600:607	arg1	NC					609:610	The prepared NC	596:610	The prepared NC	596:610	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	3	10	theme	TGA	576:578	arg1	techniques					552:561	techniques	552:561	techniques of SEM, XRD, TGA, FTIR, and EDX	552:593	The successful synthesis of NC was supported by techniques of SEM, XRD, TGA, FTIR, and EDX.					
35640686	6	11	theme	respective	1080:1089	arg1	125 mg/L					1119:1126	125 mg/L	1119:1126	125 mg/L	1119:1126	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	6	11	theme	respective	1080:1089	arg1	values					1091:1096	the respective values	1076:1096	the respective values for the factors	1076:1112	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	4	12	theme	main	658:661	arg1	parameters					673:682	the main effective parameters	654:682	the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C)	654:789	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	3	13	theme	XRD	571:573	arg1	techniques					552:561	techniques	552:561	techniques of SEM, XRD, TGA, FTIR, and EDX	552:593	The successful synthesis of NC was supported by techniques of SEM, XRD, TGA, FTIR, and EDX.					
35640686	5	14	theme	general	933:939	arg1	network					959:965	general regression neural network	933:965	general regression neural network (GRNN)	933:972	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	5	14	theme	general	933:939	arg1	GRNN					968:971	GRNN	968:971	GRNN	968:971	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	4	15	theme	TC	634:635	arg1	adsorption					637:646	TC adsorption	634:646	TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C)	634:789	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	0	16	theme	MWCNTs	64:69	arg1	MWCNTs					64:69	MWCNTs	64:69	MWCNTs	64:69	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan.					
35640686	0	16	theme	MWCNTs	64:69	arg1	nanocomposite					47:59	nanocomposite	47:59	nanocomposite of MWCNTs	47:69	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan.					
35640686	3	17	theme	FTIR	581:584	arg1	techniques					552:561	techniques	552:561	techniques of SEM, XRD, TGA, FTIR, and EDX	552:593	The successful synthesis of NC was supported by techniques of SEM, XRD, TGA, FTIR, and EDX.					
35640686	1	18	theme	pharmaceuticals	162:176	arg1	presence					150:157	The presence	146:157	The presence of pharmaceuticals as the emerging contaminates	146:205	The presence of pharmaceuticals as the emerging contaminates needs novel approaches and new materials to be remediated.					
35640686	0	19	theme	cross-linked	102:113	arg1	/chitosan					135:143	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan	87:143	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan	87:143	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan.					
35640686	5	20	theme	design	881:886	arg1	CCD					889:891	central composite design (CCD)	863:892	central composite design (CCD)	863:892	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	4	21	theme	sonication	719:728	arg1	0-8 min					736:742	0-8 min	736:742	0-8 min	736:742	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	4	21	theme	sonication	719:728	arg1	time					730:733	sonication time	719:733	sonication time (0-8 min)	719:743	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	8	22	theme	multilayer	1595:1604	arg1	nature					1606:1611	multilayer nature	1595:1611	multilayer nature	1595:1611	The kinetics, isotherms, and thermodynamic studies showed that the process is fast (over 4.5 min), chemisorption, heterogeneous with multilayer nature, spontaneous, feasible, and endothermic.					
35640686	5	23	theme	CCD	889:891	arg1	methods					852:858	different methods	842:858	different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN)	842:972	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	0	24	from	solutions	34:42	arg1	capture					13:19	Tetracycline capture	0:19	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs	0:69	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan.					
35640686	4	25	theme	TC	687:688	arg1	concentration					690:702	TC concentration	687:702	TC concentration (25-125 mg/L)	687:716	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	4	25	theme	TC	687:688	arg1	25-125 mg/L					705:715	25-125 mg/L	705:715	25-125 mg/L	705:715	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	0	26	theme	Tetracycline	0:11	arg1	capture					13:19	Tetracycline capture	0:19	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs	0:69	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan.					
35640686	4	27	theme	NC	746:747	arg1	1-130 mg					755:762	1-130 mg	755:762	1-130 mg	755:762	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	4	27	theme	NC	746:747	arg1	dose					749:752	NC dose	746:752	NC dose (1-130 mg)	746:763	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	5	28	theme	central	863:869	arg1	CCD					889:891	central composite design (CCD)	863:892	central composite design (CCD)	863:892	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	9	29	from	times	1713:1717	arg1	performance					1751:1761	its performance	1747:1761	its performance	1747:1761	In addition, the as prepared NC could be recycled for five times without significant fail in its performance.					
35640686	10	30	from	candidate	1843:1851	arg1	dealing					1856:1862	dealing	1856:1862	dealing with aqueous solutions' pollution with antibiotic	1856:1912	All in all, the developed MWCNTs/CS-PVA/GA NC can be considered as a promising candidate in dealing with aqueous solutions' pollution with antibiotic.					
35640686	7	31	theme	satisfactory	1332:1343	arg1	accuracy					1345:1352	satisfactory accuracy	1332:1352	satisfactory accuracy	1332:1352	From the models studied, although all were able to express the process with satisfactory accuracy, ANN provided the best accuracy and reliability owning to the highest R2 (0.999) and lowest RMSE, ADD, MAE.					
35640686	2	32	theme	cross-linked	342:353	arg1	nanocomposite					385:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite	327:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC)	327:419	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	2	32	theme	cross-linked	342:353	arg1	NC					417:418	MWCNTs/CS-PVA/GA NC	400:418	MWCNTs/CS-PVA/GA NC	400:418	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	1	33	theme	new	234:236	arg1	materials					238:246	new materials	234:246	new materials	234:246	The presence of pharmaceuticals as the emerging contaminates needs novel approaches and new materials to be remediated.					
35640686	10	34	theme	MWCNTs/CS-PVA/GA	1790:1805	arg1	NC					1807:1808	the developed MWCNTs/CS-PVA/GA NC	1776:1808	the developed MWCNTs/CS-PVA/GA NC	1776:1808	All in all, the developed MWCNTs/CS-PVA/GA NC can be considered as a promising candidate in dealing with aqueous solutions' pollution with antibiotic.					
35640686	10	34	theme	MWCNTs/CS-PVA/GA	1790:1805	arg1	candidate					1843:1851	a promising candidate	1831:1851	a promising candidate in dealing with aqueous solutions' pollution with antibiotic	1831:1912	All in all, the developed MWCNTs/CS-PVA/GA NC can be considered as a promising candidate in dealing with aqueous solutions' pollution with antibiotic.					
35640686	8	35	with	heterogeneous	1576:1588	arg1	nature					1606:1611	multilayer nature	1595:1611	multilayer nature	1595:1611	The kinetics, isotherms, and thermodynamic studies showed that the process is fast (over 4.5 min), chemisorption, heterogeneous with multilayer nature, spontaneous, feasible, and endothermic.					
35640686	3	36	theme	successful	508:517	arg1	synthesis					519:527	The successful synthesis	504:527	The successful synthesis of NC	504:533	The successful synthesis of NC was supported by techniques of SEM, XRD, TGA, FTIR, and EDX.					
35640686	6	37	from	130 mg	1138:1143	arg1	settings					1017:1024	the optimum settings	1005:1024	the optimum settings	1005:1024	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	5	38	theme	neural	906:911	arg1	ANN					923:925	ANN	923:925	ANN	923:925	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	5	38	theme	neural	906:911	arg1	networks					913:920	artificial neural networks	895:920	artificial neural networks (ANN)	895:926	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	8	39	dep	fast	1540:1543	arg1	spontaneous					1614:1624	spontaneous	1614:1624	spontaneous	1614:1624	The kinetics, isotherms, and thermodynamic studies showed that the process is fast (over 4.5 min), chemisorption, heterogeneous with multilayer nature, spontaneous, feasible, and endothermic.					
35640686	8	39	dep	fast	1540:1543	arg1	heterogeneous					1576:1588	heterogeneous	1576:1588	heterogeneous	1576:1588	The kinetics, isotherms, and thermodynamic studies showed that the process is fast (over 4.5 min), chemisorption, heterogeneous with multilayer nature, spontaneous, feasible, and endothermic.					
35640686	8	39	dep	fast	1540:1543	arg1	feasible					1627:1634	feasible	1627:1634	feasible	1627:1634	The kinetics, isotherms, and thermodynamic studies showed that the process is fast (over 4.5 min), chemisorption, heterogeneous with multilayer nature, spontaneous, feasible, and endothermic.					
35640686	8	39	dep	fast	1540:1543	arg1	4.5 min					1551:1557	4.5 min	1551:1557	4.5 min	1551:1557	The kinetics, isotherms, and thermodynamic studies showed that the process is fast (over 4.5 min), chemisorption, heterogeneous with multilayer nature, spontaneous, feasible, and endothermic.					
35640686	8	39	dep	fast	1540:1543	arg1	process					1529:1535	the process	1525:1535	the process	1525:1535	The kinetics, isotherms, and thermodynamic studies showed that the process is fast (over 4.5 min), chemisorption, heterogeneous with multilayer nature, spontaneous, feasible, and endothermic.					
35640686	8	39	dep	fast	1540:1543	arg1	endothermic					1641:1651	endothermic	1641:1651	endothermic	1641:1651	The kinetics, isotherms, and thermodynamic studies showed that the process is fast (over 4.5 min), chemisorption, heterogeneous with multilayer nature, spontaneous, feasible, and endothermic.					
35640686	8	39	dep	fast	1540:1543	arg1	fast					1540:1543	fast	1540:1543	fast	1540:1543	The kinetics, isotherms, and thermodynamic studies showed that the process is fast (over 4.5 min), chemisorption, heterogeneous with multilayer nature, spontaneous, feasible, and endothermic.					
35640686	8	39	dep	fast	1540:1543	arg1	chemisorption					1561:1573	chemisorption	1561:1573	chemisorption	1561:1573	The kinetics, isotherms, and thermodynamic studies showed that the process is fast (over 4.5 min), chemisorption, heterogeneous with multilayer nature, spontaneous, feasible, and endothermic.					
35640686	2	40	theme	alcohol	367:373	arg1	nanocomposite					385:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite	327:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC)	327:419	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	2	40	theme	alcohol	367:373	arg1	NC					417:418	MWCNTs/CS-PVA/GA NC	400:418	MWCNTs/CS-PVA/GA NC	400:418	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	4	41	theme	tempearure	770:779	arg1	parameters					673:682	the main effective parameters	654:682	the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C)	654:789	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	10	42	with	dealing	1856:1862	arg1	antibiotic					1903:1912	antibiotic	1903:1912	antibiotic	1903:1912	All in all, the developed MWCNTs/CS-PVA/GA NC can be considered as a promising candidate in dealing with aqueous solutions' pollution with antibiotic.					
35640686	6	43	from	125 mg/L	1119:1126	arg1	settings					1017:1024	the optimum settings	1005:1024	the optimum settings	1005:1024	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	6	44	theme	desirability	1039:1050	arg1	function					1052:1059	desirability function	1039:1059	desirability function (DA)	1039:1064	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	6	44	theme	desirability	1039:1050	arg1	DA					1062:1063	DA	1062:1063	DA	1062:1063	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	9	45	theme	prepared	1674:1681	arg1	NC					1683:1684	the as prepared NC	1667:1684	the as prepared NC	1667:1684	In addition, the as prepared NC could be recycled for five times without significant fail in its performance.					
35640686	6	46	theme	adsorption	1176:1185	arg1	capacity					1187:1194	adsorption capacity	1176:1194	adsorption capacity	1176:1194	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	1	47	dep	the	181:183	arg1	contaminates					194:205	contaminates	194:205	contaminates	194:205	The presence of pharmaceuticals as the emerging contaminates needs novel approaches and new materials to be remediated.					
35640686	8	48	theme	thermodynamic	1491:1503	arg1	studies					1505:1511	thermodynamic studies	1491:1511	thermodynamic studies	1491:1511	The kinetics, isotherms, and thermodynamic studies showed that the process is fast (over 4.5 min), chemisorption, heterogeneous with multilayer nature, spontaneous, feasible, and endothermic.					
35640686	6	49	theme	optimum	1009:1015	arg1	settings					1017:1024	the optimum settings	1005:1024	the optimum settings	1005:1024	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	10	50	theme	aqueous	1869:1875	arg1	solutions					1877:1885	aqueous solutions	1869:1885	aqueous solutions' pollution	1869:1896	All in all, the developed MWCNTs/CS-PVA/GA NC can be considered as a promising candidate in dealing with aqueous solutions' pollution with antibiotic.					
35640686	5	51	theme	network	959:965	arg1	methods					852:858	different methods	842:858	different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN)	842:972	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	9	52	dep	significant	1727:1737	arg1	fail					1739:1742	fail	1739:1742	fail	1739:1742	In addition, the as prepared NC could be recycled for five times without significant fail in its performance.					
35640686	2	53	from	solutions	493:501	arg1	MWCNTs					304:309	MWCNTs	304:309	MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC)	304:419	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	2	53	from	solutions	493:501	arg1	antibiotics					468:478	antibiotics	468:478	antibiotics from aqueous solutions	468:501	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	2	53	from	solutions	493:501	arg1	model					459:463	a model	457:463	a model of antibiotics from aqueous solutions	457:501	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	4	54	used	utilized	621:628	arg2	NC					609:610	The prepared NC	596:610	The prepared NC	596:610	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	4	55	theme	effective	663:671	arg1	parameters					673:682	the main effective parameters	654:682	the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C)	654:789	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	5	56	theme	regression	941:950	arg1	network					959:965	general regression neural network	933:965	general regression neural network (GRNN)	933:972	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	5	56	theme	regression	941:950	arg1	GRNN					968:971	GRNN	968:971	GRNN	968:971	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	2	57	theme	aqueous	485:491	arg1	solutions					493:501	aqueous solutions	485:501	aqueous solutions	485:501	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	6	58	from	6.8 min	1129:1135	arg1	settings					1017:1024	the optimum settings	1005:1024	the optimum settings	1005:1024	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	6	59	theme	NC	1199:1200	arg1	efficiency					1161:1170	efficiency	1161:1170	efficiency	1161:1170	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	6	59	theme	NC	1199:1200	arg1	capacity					1187:1194	adsorption capacity	1176:1194	adsorption capacity	1176:1194	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	0	60	theme	glutaraldehyde	87:100	arg1	/chitosan					135:143	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan	87:143	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan	87:143	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan.					
35640686	5	61	theme	process	796:802	arg1	behavior					804:811	The process behavior	792:811	The process behavior	792:811	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	7	62	theme	best	1372:1375	arg1	accuracy					1377:1384	accuracy	1377:1384	accuracy	1377:1384	From the models studied, although all were able to express the process with satisfactory accuracy, ANN provided the best accuracy and reliability owning to the highest R2 (0.999) and lowest RMSE, ADD, MAE.					
35640686	2	63	theme	antibiotics	468:478	arg1	MWCNTs					304:309	MWCNTs	304:309	MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC)	304:419	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	2	63	theme	antibiotics	468:478	arg1	model					459:463	a model	457:463	a model of antibiotics from aqueous solutions	457:501	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	0	64	theme	poly	115:118	arg1	/chitosan					135:143	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan	87:143	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan	87:143	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan.					
35640686	7	65	dep	lowest	1439:1444	arg1	ADD					1452:1454	ADD	1452:1454	ADD	1452:1454	From the models studied, although all were able to express the process with satisfactory accuracy, ANN provided the best accuracy and reliability owning to the highest R2 (0.999) and lowest RMSE, ADD, MAE.					
35640686	7	65	dep	lowest	1439:1444	arg1	RMSE					1446:1449	RMSE	1446:1449	RMSE	1446:1449	From the models studied, although all were able to express the process with satisfactory accuracy, ANN provided the best accuracy and reliability owning to the highest R2 (0.999) and lowest RMSE, ADD, MAE.					
35640686	7	65	dep	lowest	1439:1444	arg1	MAE					1457:1459	MAE	1457:1459	MAE	1457:1459	From the models studied, although all were able to express the process with satisfactory accuracy, ANN provided the best accuracy and reliability owning to the highest R2 (0.999) and lowest RMSE, ADD, MAE.					
35640686	5	66	theme	composite	871:879	arg1	CCD					889:891	central composite design (CCD)	863:892	central composite design (CCD)	863:892	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	0	67	theme	vinyl	121:125	arg1	/chitosan					135:143	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan	87:143	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan	87:143	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan.					
35640686	7	68	dep	highest	1416:1422	arg1	R2					1424:1425	R2	1424:1425	the highest R2 (0.999)	1412:1433	From the models studied, although all were able to express the process with satisfactory accuracy, ANN provided the best accuracy and reliability owning to the highest R2 (0.999) and lowest RMSE, ADD, MAE.					
35640686	7	68	dep	highest	1416:1422	arg1	0.999					1428:1432	0.999	1428:1432	0.999	1428:1432	From the models studied, although all were able to express the process with satisfactory accuracy, ANN provided the best accuracy and reliability owning to the highest R2 (0.999) and lowest RMSE, ADD, MAE.					
35640686	2	69	theme	MWCNTs/CS-PVA/GA	400:415	arg1	nanocomposite					385:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite	327:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC)	327:419	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	2	69	theme	MWCNTs/CS-PVA/GA	400:415	arg1	NC					417:418	MWCNTs/CS-PVA/GA NC	400:418	MWCNTs/CS-PVA/GA NC	400:418	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	5	70	theme	different	842:850	arg1	methods					852:858	different methods	842:858	different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN)	842:972	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	0	71	theme	aqueous	26:32	arg1	solutions					34:42	aqueous solutions	26:42	aqueous solutions	26:42	Tetracycline capture from aqueous solutions by nanocomposite of MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan.					
35640686	2	72	theme	tetracycline	436:447	arg1	removal					425:431	removal	425:431	removal of tetracycline (TC)	425:452	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	4	73	theme	concentration	690:702	arg1	parameters					673:682	the main effective parameters	654:682	the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C)	654:789	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	2	74	theme	/chitosan	375:383	arg1	nanocomposite					385:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite	327:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC)	327:419	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	2	74	theme	/chitosan	375:383	arg1	NC					417:418	MWCNTs/CS-PVA/GA NC	400:418	MWCNTs/CS-PVA/GA NC	400:418	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	5	75	theme	networks	913:920	arg1	methods					852:858	different methods	842:858	different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN)	842:972	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	3	76	theme	EDX	591:593	arg1	techniques					552:561	techniques	552:561	techniques of SEM, XRD, TGA, FTIR, and EDX	552:593	The successful synthesis of NC was supported by techniques of SEM, XRD, TGA, FTIR, and EDX.					
35640686	4	77	theme	dose	749:752	arg1	parameters					673:682	the main effective parameters	654:682	the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C)	654:789	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	2	78	theme	poly	355:358	arg1	nanocomposite					385:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite	327:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC)	327:419	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	2	78	theme	poly	355:358	arg1	NC					417:418	MWCNTs/CS-PVA/GA NC	400:418	MWCNTs/CS-PVA/GA NC	400:418	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	6	79	from	45 °C	1150:1154	arg1	settings					1017:1024	the optimum settings	1005:1024	the optimum settings	1005:1024	The results showed that under the optimum settings presented by desirability function (DA), in which the respective values for the factors were 125 mg/L, 6.8 min, 130 mg, and 45 °C, the efficiency and adsorption capacity of NC is supposed to be 99.07% and ∼525 mg/g, respectively.					
35640686	4	80	theme	time	730:733	arg1	parameters					673:682	the main effective parameters	654:682	the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C)	654:789	The prepared NC was then utilized for TC adsorption under the main effective parameters of TC concentration (25-125 mg/L), sonication time (0-8 min), NC dose (1-130 mg), and tempearure (5-45 °C).					
35640686	2	81	theme	glutaraldehyde	327:340	arg1	nanocomposite					385:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite	327:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC)	327:419	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	2	81	theme	glutaraldehyde	327:340	arg1	NC					417:418	MWCNTs/CS-PVA/GA NC	400:418	MWCNTs/CS-PVA/GA NC	400:418	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	5	82	theme	artificial	895:904	arg1	ANN					923:925	ANN	923:925	ANN	923:925	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	5	82	theme	artificial	895:904	arg1	networks					913:920	artificial neural networks	895:920	artificial neural networks (ANN)	895:926	The process behavior was comparably explored with different methods of central composite design (CCD), artificial neural networks (ANN), and general regression neural network (GRNN).					
35640686	2	83	from	model	459:463	arg1	solutions					493:501	aqueous solutions	485:501	aqueous solutions	485:501	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	10	84	theme	developed	1780:1788	arg1	NC					1807:1808	the developed MWCNTs/CS-PVA/GA NC	1776:1808	the developed MWCNTs/CS-PVA/GA NC	1776:1808	All in all, the developed MWCNTs/CS-PVA/GA NC can be considered as a promising candidate in dealing with aqueous solutions' pollution with antibiotic.					
35640686	10	84	theme	developed	1780:1788	arg1	candidate					1843:1851	a promising candidate	1831:1851	a promising candidate in dealing with aqueous solutions' pollution with antibiotic	1831:1912	All in all, the developed MWCNTs/CS-PVA/GA NC can be considered as a promising candidate in dealing with aqueous solutions' pollution with antibiotic.					
35640686	2	85	link	cross-linked	342:353	arg1	nanocomposite					385:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite	327:397	glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC)	327:419	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
35640686	2	85	link	cross-linked	342:353	arg1	NC					417:418	MWCNTs/CS-PVA/GA NC	400:418	MWCNTs/CS-PVA/GA NC	400:418	This study aimed to develop and apply MWCNTs reinforced with glutaraldehyde cross-linked poly (vinyl alcohol)/chitosan nanocomposite (MWCNTs/CS-PVA/GA NC) for removal of tetracycline (TC) as a model of antibiotics from aqueous solutions.					
36574638	0	0	from	Oligosaccharides	12:27	arg1	Products					57:64	Korean Fermented Soybean Products	32:64	Korean Fermented Soybean Products	32:64	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.					
36574638	6	1	theme	great	939:943	arg1	diversity					956:964	The great structural diversity	935:964	The great structural diversity of the oligosaccharides found	935:994	The great structural diversity of the oligosaccharides found suggests that soybean carbohydrates are hydrolyzed and/or transformed during fermentation and may yield novel oligosaccharides.					
36574638	2	2	contain	have	318:321	arg1	oligosaccharides					260:275	The oligosaccharides	256:275	The oligosaccharides found in soybean and its fermented foods	256:316	The oligosaccharides found in soybean and its fermented foods have great potential to improve the quality of foods; however, their structural details have not been well studied.					
36574638	2	2	contain	have	318:321	arg2	potential					329:337	great potential	323:337	great potential to improve the quality of foods	323:369	The oligosaccharides found in soybean and its fermented foods have great potential to improve the quality of foods; however, their structural details have not been well studied.					
36574638	3	3	theme	advanced	457:464	arg1	spectrometry					471:482	advanced mass spectrometry	457:482	advanced mass spectrometry	457:482	In this study, we used advanced mass spectrometry and gas chromatography to analyze oligosaccharides and their monomeric composition in two fermented soybean products.					
36574638	0	4	theme	Spectrometry	93:104	arg1	Combination					73:83	the Combination	69:83	the Combination of Mass Spectrometry and Gas Chromatography	69:127	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.					
36574638	0	5	from	Analysis	0:7	arg1	Products					57:64	Korean Fermented Soybean Products	32:64	Korean Fermented Soybean Products	32:64	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.					
36574638	6	6	theme	structural	945:954	arg1	diversity					956:964	The great structural diversity	935:964	The great structural diversity of the oligosaccharides found	935:994	The great structural diversity of the oligosaccharides found suggests that soybean carbohydrates are hydrolyzed and/or transformed during fermentation and may yield novel oligosaccharides.					
36574638	0	7	theme	Mass	88:91	arg1	Spectrometry					93:104	Mass Spectrometry	88:104	Mass Spectrometry	88:104	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.					
36574638	0	8	from	Products	57:64	arg1	Analysis					0:7	Analysis	0:7	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.	0:128	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.					
36574638	4	9	theme	polymerization	651:664	arg1	degree					641:646	a degree	639:646	a degree of polymerization ranging from 3 to 7	639:684	In both foods, oligosaccharides with a degree of polymerization ranging from 3 to 7 were found.					
36574638	6	10	theme	novel	1100:1104	arg1	oligosaccharides					1106:1121	novel oligosaccharides	1100:1121	novel oligosaccharides	1100:1121	The great structural diversity of the oligosaccharides found suggests that soybean carbohydrates are hydrolyzed and/or transformed during fermentation and may yield novel oligosaccharides.					
36574638	0	11	theme	Gas	110:112	arg1	Chromatography					114:127	Gas Chromatography	110:127	Gas Chromatography	110:127	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.					
36574638	5	12	theme	constituent	704:714	arg1	monosaccharides					716:730	Their constituent monosaccharides	698:730	Their constituent monosaccharides	698:730	Their constituent monosaccharides were characterized; galactose, xylose, arabinose, and rhamnose were the predominant constituents of the oligosaccharides, and fucose, fructose, mannose, glucose, and N-acetylglucosamine were also found.					
36574638	3	13	theme	gas	488:490	arg1	chromatography					492:505	gas chromatography	488:505	gas chromatography	488:505	In this study, we used advanced mass spectrometry and gas chromatography to analyze oligosaccharides and their monomeric composition in two fermented soybean products.					
36574638	2	14	theme	foods	365:369	arg1	quality					354:360	the quality	350:360	the quality of foods	350:369	The oligosaccharides found in soybean and its fermented foods have great potential to improve the quality of foods; however, their structural details have not been well studied.					
36574638	5	15	theme	oligosaccharides	836:851	arg1	xylose					763:768	xylose	763:768	xylose	763:768	Their constituent monosaccharides were characterized; galactose, xylose, arabinose, and rhamnose were the predominant constituents of the oligosaccharides, and fucose, fructose, mannose, glucose, and N-acetylglucosamine were also found.					
36574638	5	15	theme	oligosaccharides	836:851	arg1	galactose					752:760	galactose	752:760	galactose	752:760	Their constituent monosaccharides were characterized; galactose, xylose, arabinose, and rhamnose were the predominant constituents of the oligosaccharides, and fucose, fructose, mannose, glucose, and N-acetylglucosamine were also found.					
36574638	5	15	theme	oligosaccharides	836:851	arg1	constituents					816:827	the predominant constituents	800:827	the predominant constituents of the oligosaccharides	800:851	Their constituent monosaccharides were characterized; galactose, xylose, arabinose, and rhamnose were the predominant constituents of the oligosaccharides, and fucose, fructose, mannose, glucose, and N-acetylglucosamine were also found.					
36574638	5	15	theme	oligosaccharides	836:851	arg1	arabinose					771:779	arabinose	771:779	arabinose	771:779	Their constituent monosaccharides were characterized; galactose, xylose, arabinose, and rhamnose were the predominant constituents of the oligosaccharides, and fucose, fructose, mannose, glucose, and N-acetylglucosamine were also found.					
36574638	5	15	theme	oligosaccharides	836:851	arg1	rhamnose					786:793	rhamnose	786:793	rhamnose	786:793	Their constituent monosaccharides were characterized; galactose, xylose, arabinose, and rhamnose were the predominant constituents of the oligosaccharides, and fucose, fructose, mannose, glucose, and N-acetylglucosamine were also found.					
36574638	4	16	dep	7	684:684	arg1	to					681:682	to	681:682	to	681:682	In both foods, oligosaccharides with a degree of polymerization ranging from 3 to 7 were found.					
36574638	1	17	theme	soy	178:180	arg1	ganjang					169:175	ganjang	169:175	ganjang (soy sauce)	169:187	Doenjang (fermented soybean paste) and ganjang (soy sauce) are traditional fermented foods that are widely consumed in Korea.					
36574638	1	17	theme	soy	178:180	arg1	sauce					182:186	soy sauce	178:186	soy sauce	178:186	Doenjang (fermented soybean paste) and ganjang (soy sauce) are traditional fermented foods that are widely consumed in Korea.					
36574638	3	18	from	composition	555:565	arg1	products					592:599	two fermented soybean products	570:599	two fermented soybean products	570:599	In this study, we used advanced mass spectrometry and gas chromatography to analyze oligosaccharides and their monomeric composition in two fermented soybean products.					
36574638	2	19	theme	structural	387:396	arg1	details					398:404	their structural details	381:404	their structural details	381:404	The oligosaccharides found in soybean and its fermented foods have great potential to improve the quality of foods; however, their structural details have not been well studied.					
36574638	0	20	theme	Oligosaccharides	12:27	arg1	Analysis					0:7	Analysis	0:7	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.	0:128	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.					
36574638	0	21	theme	Chromatography	114:127	arg1	Combination					73:83	the Combination	69:83	the Combination of Mass Spectrometry and Gas Chromatography	69:127	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.					
36574638	3	22	theme	mass	466:469	arg1	spectrometry					471:482	advanced mass spectrometry	457:482	advanced mass spectrometry	457:482	In this study, we used advanced mass spectrometry and gas chromatography to analyze oligosaccharides and their monomeric composition in two fermented soybean products.					
36574638	0	23	theme	Fermented	39:47	arg1	Products					57:64	Korean Fermented Soybean Products	32:64	Korean Fermented Soybean Products	32:64	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.					
36574638	6	24	theme	soybean	1010:1016	arg1	carbohydrates					1018:1030	soybean carbohydrates	1010:1030	soybean carbohydrates	1010:1030	The great structural diversity of the oligosaccharides found suggests that soybean carbohydrates are hydrolyzed and/or transformed during fermentation and may yield novel oligosaccharides.					
36574638	5	25	theme	predominant	804:814	arg1	xylose					763:768	xylose	763:768	xylose	763:768	Their constituent monosaccharides were characterized; galactose, xylose, arabinose, and rhamnose were the predominant constituents of the oligosaccharides, and fucose, fructose, mannose, glucose, and N-acetylglucosamine were also found.					
36574638	5	25	theme	predominant	804:814	arg1	galactose					752:760	galactose	752:760	galactose	752:760	Their constituent monosaccharides were characterized; galactose, xylose, arabinose, and rhamnose were the predominant constituents of the oligosaccharides, and fucose, fructose, mannose, glucose, and N-acetylglucosamine were also found.					
36574638	5	25	theme	predominant	804:814	arg1	constituents					816:827	the predominant constituents	800:827	the predominant constituents of the oligosaccharides	800:851	Their constituent monosaccharides were characterized; galactose, xylose, arabinose, and rhamnose were the predominant constituents of the oligosaccharides, and fucose, fructose, mannose, glucose, and N-acetylglucosamine were also found.					
36574638	5	25	theme	predominant	804:814	arg1	arabinose					771:779	arabinose	771:779	arabinose	771:779	Their constituent monosaccharides were characterized; galactose, xylose, arabinose, and rhamnose were the predominant constituents of the oligosaccharides, and fucose, fructose, mannose, glucose, and N-acetylglucosamine were also found.					
36574638	5	25	theme	predominant	804:814	arg1	rhamnose					786:793	rhamnose	786:793	rhamnose	786:793	Their constituent monosaccharides were characterized; galactose, xylose, arabinose, and rhamnose were the predominant constituents of the oligosaccharides, and fucose, fructose, mannose, glucose, and N-acetylglucosamine were also found.					
36574638	0	26	theme	Korean	32:37	arg1	Products					57:64	Korean Fermented Soybean Products	32:64	Korean Fermented Soybean Products	32:64	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.					
36574638	4	27	located	found	691:695	arg1	foods					610:614	both foods	605:614	both foods	605:614	In both foods, oligosaccharides with a degree of polymerization ranging from 3 to 7 were found.					
36574638	4	27	located	found	691:695	arg2	oligosaccharides					617:632	oligosaccharides	617:632	oligosaccharides with a degree of polymerization ranging from 3 to 7	617:684	In both foods, oligosaccharides with a degree of polymerization ranging from 3 to 7 were found.					
36574638	1	28	theme	traditional	193:203	arg1	Doenjang					130:137	Doenjang	130:137	Doenjang (fermented soybean paste)	130:163	Doenjang (fermented soybean paste) and ganjang (soy sauce) are traditional fermented foods that are widely consumed in Korea.					
36574638	1	28	theme	traditional	193:203	arg1	foods					215:219	traditional fermented foods	193:219	traditional fermented foods that are widely consumed in Korea	193:253	Doenjang (fermented soybean paste) and ganjang (soy sauce) are traditional fermented foods that are widely consumed in Korea.					
36574638	1	28	theme	traditional	193:203	arg1	ganjang					169:175	ganjang	169:175	ganjang (soy sauce)	169:187	Doenjang (fermented soybean paste) and ganjang (soy sauce) are traditional fermented foods that are widely consumed in Korea.					
36574638	2	29	located	found	277:281	arg1	soybean					286:292	soybean	286:292	soybean	286:292	The oligosaccharides found in soybean and its fermented foods have great potential to improve the quality of foods; however, their structural details have not been well studied.					
36574638	2	29	located	found	277:281	arg1	foods					312:316	its fermented foods	298:316	its fermented foods	298:316	The oligosaccharides found in soybean and its fermented foods have great potential to improve the quality of foods; however, their structural details have not been well studied.					
36574638	2	29	located	found	277:281	arg2	oligosaccharides					260:275	The oligosaccharides	256:275	The oligosaccharides found in soybean and its fermented foods	256:316	The oligosaccharides found in soybean and its fermented foods have great potential to improve the quality of foods; however, their structural details have not been well studied.					
36574638	4	30	with	oligosaccharides	617:632	arg1	degree					641:646	a degree	639:646	a degree of polymerization ranging from 3 to 7	639:684	In both foods, oligosaccharides with a degree of polymerization ranging from 3 to 7 were found.					
36574638	3	31	theme	monomeric	545:553	arg1	composition					555:565	their monomeric composition	539:565	their monomeric composition in two fermented soybean products	539:599	In this study, we used advanced mass spectrometry and gas chromatography to analyze oligosaccharides and their monomeric composition in two fermented soybean products.					
36574638	2	32	theme	great	323:327	arg1	potential					329:337	great potential	323:337	great potential to improve the quality of foods	323:369	The oligosaccharides found in soybean and its fermented foods have great potential to improve the quality of foods; however, their structural details have not been well studied.					
36574638	0	33	theme	Soybean	49:55	arg1	Products					57:64	Korean Fermented Soybean Products	32:64	Korean Fermented Soybean Products	32:64	Analysis of Oligosaccharides in Korean Fermented Soybean Products by the Combination of Mass Spectrometry and Gas Chromatography.					
36574638	3	34	from	oligosaccharides	518:533	arg1	products					592:599	two fermented soybean products	570:599	two fermented soybean products	570:599	In this study, we used advanced mass spectrometry and gas chromatography to analyze oligosaccharides and their monomeric composition in two fermented soybean products.					
36574638	1	35	theme	fermented	205:213	arg1	Doenjang					130:137	Doenjang	130:137	Doenjang (fermented soybean paste)	130:163	Doenjang (fermented soybean paste) and ganjang (soy sauce) are traditional fermented foods that are widely consumed in Korea.					
36574638	1	35	theme	fermented	205:213	arg1	foods					215:219	traditional fermented foods	193:219	traditional fermented foods that are widely consumed in Korea	193:253	Doenjang (fermented soybean paste) and ganjang (soy sauce) are traditional fermented foods that are widely consumed in Korea.					
36574638	1	35	theme	fermented	205:213	arg1	ganjang					169:175	ganjang	169:175	ganjang (soy sauce)	169:187	Doenjang (fermented soybean paste) and ganjang (soy sauce) are traditional fermented foods that are widely consumed in Korea.					
36574638	3	36	used	used	452:455	arg2	we					449:450	we	449:450	we	449:450	In this study, we used advanced mass spectrometry and gas chromatography to analyze oligosaccharides and their monomeric composition in two fermented soybean products.					
36574638	2	37	theme	fermented	302:310	arg1	foods					312:316	its fermented foods	298:316	its fermented foods	298:316	The oligosaccharides found in soybean and its fermented foods have great potential to improve the quality of foods; however, their structural details have not been well studied.					
36574638	3	38	theme	fermented	574:582	arg1	products					592:599	two fermented soybean products	570:599	two fermented soybean products	570:599	In this study, we used advanced mass spectrometry and gas chromatography to analyze oligosaccharides and their monomeric composition in two fermented soybean products.					
36574638	1	39	theme	fermented	140:148	arg1	Doenjang					130:137	Doenjang	130:137	Doenjang (fermented soybean paste)	130:163	Doenjang (fermented soybean paste) and ganjang (soy sauce) are traditional fermented foods that are widely consumed in Korea.					
36574638	1	39	theme	fermented	140:148	arg1	paste					158:162	fermented soybean paste	140:162	fermented soybean paste	140:162	Doenjang (fermented soybean paste) and ganjang (soy sauce) are traditional fermented foods that are widely consumed in Korea.					
36574638	6	40	theme	oligosaccharides	973:988	arg1	diversity					956:964	The great structural diversity	935:964	The great structural diversity of the oligosaccharides found	935:994	The great structural diversity of the oligosaccharides found suggests that soybean carbohydrates are hydrolyzed and/or transformed during fermentation and may yield novel oligosaccharides.					
36574638	3	41	theme	soybean	584:590	arg1	products					592:599	two fermented soybean products	570:599	two fermented soybean products	570:599	In this study, we used advanced mass spectrometry and gas chromatography to analyze oligosaccharides and their monomeric composition in two fermented soybean products.					
36574638	1	42	theme	soybean	150:156	arg1	Doenjang					130:137	Doenjang	130:137	Doenjang (fermented soybean paste)	130:163	Doenjang (fermented soybean paste) and ganjang (soy sauce) are traditional fermented foods that are widely consumed in Korea.					
36574638	1	42	theme	soybean	150:156	arg1	paste					158:162	fermented soybean paste	140:162	fermented soybean paste	140:162	Doenjang (fermented soybean paste) and ganjang (soy sauce) are traditional fermented foods that are widely consumed in Korea.					
36235583	8	0	theme	anti-inflammatory	1074:1090	arg1	IL-10					1103:1107	IL-10	1103:1107	IL-10	1103:1107	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	8	0	theme	anti-inflammatory	1074:1090	arg1	cytokines					1092:1100	increased anti-inflammatory cytokines	1064:1100	increased anti-inflammatory cytokines (IL-10)	1064:1108	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	9	1	theme	gene	1279:1282	arg1	Sirt1					1284:1288	anti-aging gene Sirt1	1268:1288	anti-aging gene Sirt1	1268:1288	In addition, APF, APS, and APRS significantly downregulated the relative mRNA expression of senescence-associated gene p53 and upregulated the expression of anti-aging gene Sirt1.					
36235583	5	2	theme	APRS	596:599	arg1	supplementation					601:615	APRS supplementation	596:615	APRS supplementation	596:615	APRS supplementation led to a greatest increasement in abundance of Lactobacillus, Roseburia, and Faecalibacterium prausnitzii.					
36235583	2	3	theme	prebiotic	424:432	arg1	effects					434:440	different prebiotic effects	414:440	different prebiotic effects	414:440	APF, APS, and APRS altered gut microbiota composition and exhibited different prebiotic effects.					
36235583	9	4	theme	gene	1225:1228	arg1	p53					1230:1232	senescence-associated gene p53	1203:1232	senescence-associated gene p53	1203:1232	In addition, APF, APS, and APRS significantly downregulated the relative mRNA expression of senescence-associated gene p53 and upregulated the expression of anti-aging gene Sirt1.					
36235583	10	5	theme	products	1391:1398	arg1	effects					1365:1371	the beneficial effects	1350:1371	the beneficial effects of Arenga pinnata products on human health	1350:1414	These results provide potentially useful information about the beneficial effects of Arenga pinnata products on human health.					
36235583	1	6	from	effects	152:158	arg1	improvement					293:303	improvement	293:303	improvement of intestinal inflammation in aged mice	293:343	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	1	6	from	effects	152:158	arg1	microbiota					278:287	gut microbiota	274:287	gut microbiota	274:287	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	10	7	from	effects	1365:1371	arg1	health					1409:1414	human health	1403:1414	human health	1403:1414	These results provide potentially useful information about the beneficial effects of Arenga pinnata products on human health.					
36235583	5	8	theme	Roseburia	679:687	arg1	abundance					651:659	abundance	651:659	abundance of Lactobacillus, Roseburia, and Faecalibacterium prausnitzii	651:721	APRS supplementation led to a greatest increasement in abundance of Lactobacillus, Roseburia, and Faecalibacterium prausnitzii.					
36235583	9	9	theme	p53	1230:1232	arg1	expression					1189:1198	the relative mRNA expression	1171:1198	the relative mRNA expression of senescence-associated gene p53	1171:1232	In addition, APF, APS, and APRS significantly downregulated the relative mRNA expression of senescence-associated gene p53 and upregulated the expression of anti-aging gene Sirt1.					
36235583	8	10	theme	relative	980:987	arg1	expression					994:1003	relative mRNA expression	980:1003	relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10)	980:1108	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	7	11	theme	ameliorative	909:920	arg1	effect					922:927	ameliorative effect	909:927	ameliorative effect	909:927	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	4	12	theme	genus	536:540	arg1	Lachnospiraceae_NK4A136					542:564	genus Lachnospiraceae_NK4A136	536:564	genus Lachnospiraceae_NK4A136	536:564	The abundance of genus Lachnospiraceae_NK4A136 was highest in the APS group.					
36235583	0	13	theme	Aged	98:101	arg1	Mice					103:106	Aged Mice	98:106	Aged Mice	98:106	Arenga pinnata Resistant Starch Modulate Gut Microbiota and Ameliorate Intestinal Inflammation in Aged Mice.					
36235583	8	14	dep	cytokines	1025:1033	arg1	IL-6					1036:1039	IL-6	1036:1039	IL-6	1036:1039	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	8	14	dep	cytokines	1025:1033	arg1	cytokines					1025:1033	pro-inflammatory cytokines	1008:1033	pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α)	1008:1058	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	8	14	dep	cytokines	1025:1033	arg1	IL-1β					1042:1046	IL-1β	1042:1046	IL-1β	1042:1046	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	8	14	dep	cytokines	1025:1033	arg1	TNF-α					1053:1057	TNF-α	1053:1057	TNF-α	1053:1057	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	10	15	theme	Arenga	1376:1381	arg1	products					1391:1398	Arenga pinnata products	1376:1398	Arenga pinnata products	1376:1398	These results provide potentially useful information about the beneficial effects of Arenga pinnata products on human health.					
36235583	10	16	theme	useful	1325:1330	arg1	information					1332:1342	potentially useful information	1313:1342	potentially useful information about the beneficial effects of Arenga pinnata products on human health	1313:1414	These results provide potentially useful information about the beneficial effects of Arenga pinnata products on human health.					
36235583	3	17	theme	aged	500:503	arg1	mice					505:508	aged mice	500:508	aged mice fed APF	500:516	Bifidobacterium showed the greatest increase in feces of aged mice fed APF.					
36235583	1	18	theme	intestinal	308:317	arg1	inflammation					319:330	intestinal inflammation	308:330	intestinal inflammation	308:330	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	3	19	from	increase	479:486	arg1	feces					491:495	feces	491:495	feces of aged mice fed APF	491:516	Bifidobacterium showed the greatest increase in feces of aged mice fed APF.					
36235583	1	20	theme	Arenga	163:168	arg1	APRS					198:201	APRS	198:201	APRS	198:201	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	1	20	theme	Arenga	163:168	arg1	starch					190:195	Arenga pinnata retrograded starch	163:195	Arenga pinnata retrograded starch (APRS)	163:202	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	5	21	theme	Faecalibacterium	694:709	arg1	abundance					651:659	abundance	651:659	abundance of Lactobacillus, Roseburia, and Faecalibacterium prausnitzii	651:721	APRS supplementation led to a greatest increasement in abundance of Lactobacillus, Roseburia, and Faecalibacterium prausnitzii.					
36235583	1	22	theme	inflammation	319:330	arg1	improvement					293:303	improvement	293:303	improvement of intestinal inflammation in aged mice	293:343	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	1	22	theme	inflammation	319:330	arg1	microbiota					278:287	gut microbiota	274:287	gut microbiota	274:287	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	2	23	theme	different	414:422	arg1	effects					434:440	different prebiotic effects	414:440	different prebiotic effects	414:440	APF, APS, and APRS altered gut microbiota composition and exhibited different prebiotic effects.					
36235583	6	24	theme	fatty	768:772	arg1	SCFAs					780:784	SCFAs	780:784	SCFAs	780:784	APRS induced significantly more short-chain fatty acid (SCFAs) production than APF and APS.					
36235583	6	24	theme	fatty	768:772	arg1	acid					774:777	short-chain fatty acid	756:777	significantly more short-chain fatty acid (SCFAs) production	737:796	APRS induced significantly more short-chain fatty acid (SCFAs) production than APF and APS.					
36235583	2	25	theme	gut	373:375	arg1	composition					388:398	gut microbiota composition	373:398	gut microbiota composition	373:398	APF, APS, and APRS altered gut microbiota composition and exhibited different prebiotic effects.					
36235583	1	26	theme	pinnata	170:176	arg1	APRS					198:201	APRS	198:201	APRS	198:201	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	1	26	theme	pinnata	170:176	arg1	starch					190:195	Arenga pinnata retrograded starch	163:195	Arenga pinnata retrograded starch (APRS)	163:202	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	0	27	theme	pinnata	7:13	arg1	Starch					25:30	Arenga pinnata Resistant Starch	0:30	Arenga pinnata Resistant Starch	0:30	Arenga pinnata Resistant Starch Modulate Gut Microbiota and Ameliorate Intestinal Inflammation in Aged Mice.					
36235583	8	28	theme	increased	1064:1072	arg1	IL-10					1103:1107	IL-10	1103:1107	IL-10	1103:1107	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	8	28	theme	increased	1064:1072	arg1	cytokines					1092:1100	increased anti-inflammatory cytokines	1064:1100	increased anti-inflammatory cytokines (IL-10)	1064:1108	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	8	29	theme	pro-inflammatory	1008:1023	arg1	IL-6					1036:1039	IL-6	1036:1039	IL-6	1036:1039	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	8	29	theme	pro-inflammatory	1008:1023	arg1	cytokines					1025:1033	pro-inflammatory cytokines	1008:1033	pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α)	1008:1058	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	8	29	theme	pro-inflammatory	1008:1023	arg1	IL-1β					1042:1046	IL-1β	1042:1046	IL-1β	1042:1046	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	8	29	theme	pro-inflammatory	1008:1023	arg1	TNF-α					1053:1057	TNF-α	1053:1057	TNF-α	1053:1057	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	6	30	theme	short-chain	756:766	arg1	SCFAs					780:784	SCFAs	780:784	SCFAs	780:784	APRS induced significantly more short-chain fatty acid (SCFAs) production than APF and APS.					
36235583	6	30	theme	short-chain	756:766	arg1	acid					774:777	short-chain fatty acid	756:777	significantly more short-chain fatty acid (SCFAs) production	737:796	APRS induced significantly more short-chain fatty acid (SCFAs) production than APF and APS.					
36235583	10	31	theme	beneficial	1354:1363	arg1	effects					1365:1371	the beneficial effects	1350:1371	the beneficial effects of Arenga pinnata products on human health	1350:1414	These results provide potentially useful information about the beneficial effects of Arenga pinnata products on human health.					
36235583	1	32	theme	retrograded	178:188	arg1	APRS					198:201	APRS	198:201	APRS	198:201	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	1	32	theme	retrograded	178:188	arg1	starch					190:195	Arenga pinnata retrograded starch	163:195	Arenga pinnata retrograded starch (APRS)	163:202	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	0	33	theme	Arenga	0:5	arg1	Starch					25:30	Arenga pinnata Resistant Starch	0:30	Arenga pinnata Resistant Starch	0:30	Arenga pinnata Resistant Starch Modulate Gut Microbiota and Ameliorate Intestinal Inflammation in Aged Mice.					
36235583	1	34	theme	whole	238:242	arg1	APF					266:268	APF	266:268	APF	266:268	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	1	34	theme	whole	238:242	arg1	flour					259:263	whole Arenga pinnata flour	238:263	whole Arenga pinnata flour (APF)	238:269	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	10	35	theme	human	1403:1407	arg1	health					1409:1414	human health	1403:1414	human health	1403:1414	These results provide potentially useful information about the beneficial effects of Arenga pinnata products on human health.					
36235583	6	36	theme	more	751:754	arg1	production					787:796	significantly more short-chain fatty acid (SCFAs) production	737:796	significantly more short-chain fatty acid (SCFAs) production	737:796	APRS induced significantly more short-chain fatty acid (SCFAs) production than APF and APS.					
36235583	1	37	theme	starch	190:195	arg1	effects					152:158	the regulatory effects	137:158	the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice	137:343	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	4	38	theme	Lachnospiraceae_NK4A136	542:564	arg1	highest					570:576	highest	570:576	highest	570:576	The abundance of genus Lachnospiraceae_NK4A136 was highest in the APS group.					
36235583	4	38	theme	Lachnospiraceae_NK4A136	542:564	arg1	abundance					523:531	The abundance	519:531	The abundance of genus Lachnospiraceae_NK4A136	519:564	The abundance of genus Lachnospiraceae_NK4A136 was highest in the APS group.					
36235583	1	39	theme	Arenga	244:249	arg1	APF					266:268	APF	266:268	APF	266:268	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	1	39	theme	Arenga	244:249	arg1	flour					259:263	whole Arenga pinnata flour	238:263	whole Arenga pinnata flour (APF)	238:269	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	6	40	theme	acid	774:777	arg1	production					787:796	significantly more short-chain fatty acid (SCFAs) production	737:796	significantly more short-chain fatty acid (SCFAs) production	737:796	APRS induced significantly more short-chain fatty acid (SCFAs) production than APF and APS.					
36235583	3	41	theme	mice	505:508	arg1	feces					491:495	feces	491:495	feces of aged mice fed APF	491:516	Bifidobacterium showed the greatest increase in feces of aged mice fed APF.					
36235583	4	42	theme	APS	585:587	arg1	group					589:593	the APS group	581:593	the APS group	581:593	The abundance of genus Lachnospiraceae_NK4A136 was highest in the APS group.					
36235583	0	43	theme	Resistant	15:23	arg1	Starch					25:30	Arenga pinnata Resistant Starch	0:30	Arenga pinnata Resistant Starch	0:30	Arenga pinnata Resistant Starch Modulate Gut Microbiota and Ameliorate Intestinal Inflammation in Aged Mice.					
36235583	1	44	theme	pinnata	251:257	arg1	APF					266:268	APF	266:268	APF	266:268	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	1	44	theme	pinnata	251:257	arg1	flour					259:263	whole Arenga pinnata flour	238:263	whole Arenga pinnata flour (APF)	238:269	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	7	45	dep	APF	816:818	arg1	treatments					835:844	treatments	835:844	treatments	835:844	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	1	46	theme	aged	335:338	arg1	mice					340:343	aged mice	335:343	aged mice	335:343	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	7	47	from	inflammation	866:877	arg1	mice					887:890	aged mice	882:890	aged mice	882:890	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	0	48	theme	Gut	41:43	arg1	Microbiota					45:54	Gut Microbiota	41:54	Gut Microbiota	41:54	Arenga pinnata Resistant Starch Modulate Gut Microbiota and Ameliorate Intestinal Inflammation in Aged Mice.					
36235583	1	49	theme	flour	259:263	arg1	effects					152:158	the regulatory effects	137:158	the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice	137:343	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	5	50	theme	Lactobacillus	664:676	arg1	abundance					651:659	abundance	651:659	abundance of Lactobacillus, Roseburia, and Faecalibacterium prausnitzii	651:721	APRS supplementation led to a greatest increasement in abundance of Lactobacillus, Roseburia, and Faecalibacterium prausnitzii.					
36235583	0	51	from	Inflammation	82:93	arg1	Mice					103:106	Aged Mice	98:106	Aged Mice	98:106	Arenga pinnata Resistant Starch Modulate Gut Microbiota and Ameliorate Intestinal Inflammation in Aged Mice.					
36235583	8	52	theme	cytokines	1025:1033	arg1	expression					994:1003	relative mRNA expression	980:1003	relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10)	980:1108	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	1	53	from	improvement	293:303	arg1	mice					340:343	aged mice	335:343	aged mice	335:343	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	9	54	theme	anti-aging	1268:1277	arg1	Sirt1					1284:1288	anti-aging gene Sirt1	1268:1288	anti-aging gene Sirt1	1268:1288	In addition, APF, APS, and APRS significantly downregulated the relative mRNA expression of senescence-associated gene p53 and upregulated the expression of anti-aging gene Sirt1.					
36235583	9	55	theme	senescence-associated	1203:1223	arg1	p53					1230:1232	senescence-associated gene p53	1203:1232	senescence-associated gene p53	1203:1232	In addition, APF, APS, and APRS significantly downregulated the relative mRNA expression of senescence-associated gene p53 and upregulated the expression of anti-aging gene Sirt1.					
36235583	1	56	from	microbiota	278:287	arg1	mice					340:343	aged mice	335:343	aged mice	335:343	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	5	57	theme	greatest	626:633	arg1	increasement					635:646	a greatest increasement	624:646	a greatest increasement in abundance of Lactobacillus, Roseburia, and Faecalibacterium prausnitzii	624:721	APRS supplementation led to a greatest increasement in abundance of Lactobacillus, Roseburia, and Faecalibacterium prausnitzii.					
36235583	8	58	theme	mRNA	989:992	arg1	expression					994:1003	relative mRNA expression	980:1003	relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10)	980:1108	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36235583	7	59	theme	APS	940:942	arg1	order					900:904	the order	896:904	the order of ameliorative effect	896:927	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	7	59	theme	APS	940:942	arg1	APF					946:948	APRS > APS > APF	933:948	APRS > APS > APF	933:948	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	10	60	theme	pinnata	1383:1389	arg1	products					1391:1398	Arenga pinnata products	1376:1398	Arenga pinnata products	1376:1398	These results provide potentially useful information about the beneficial effects of Arenga pinnata products on human health.					
36235583	7	61	theme	intestinal	855:864	arg1	inflammation					866:877	intestinal inflammation	855:877	intestinal inflammation in aged mice	855:890	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	7	62	theme	aged	882:885	arg1	mice					887:890	aged mice	882:890	aged mice	882:890	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	9	63	theme	relative	1175:1182	arg1	expression					1189:1198	the relative mRNA expression	1171:1198	the relative mRNA expression of senescence-associated gene p53	1171:1232	In addition, APF, APS, and APRS significantly downregulated the relative mRNA expression of senescence-associated gene p53 and upregulated the expression of anti-aging gene Sirt1.					
36235583	9	64	theme	Sirt1	1284:1288	arg1	expression					1254:1263	the expression	1250:1263	the expression of anti-aging gene Sirt1	1250:1288	In addition, APF, APS, and APRS significantly downregulated the relative mRNA expression of senescence-associated gene p53 and upregulated the expression of anti-aging gene Sirt1.					
36235583	7	65	theme	>	944:944	arg1	order					900:904	the order	896:904	the order of ameliorative effect	896:927	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	7	65	theme	>	944:944	arg1	APF					946:948	APRS > APS > APF	933:948	APRS > APS > APF	933:948	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	5	66	from	increasement	635:646	arg1	abundance					651:659	abundance	651:659	abundance of Lactobacillus, Roseburia, and Faecalibacterium prausnitzii	651:721	APRS supplementation led to a greatest increasement in abundance of Lactobacillus, Roseburia, and Faecalibacterium prausnitzii.					
36235583	1	67	theme	Arenga	205:210	arg1	starch					220:225	Arenga pinnata starch	205:225	Arenga pinnata starch (APS)	205:231	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	1	67	theme	Arenga	205:210	arg1	APS					228:230	APS	228:230	APS	228:230	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	9	68	theme	mRNA	1184:1187	arg1	expression					1189:1198	the relative mRNA expression	1171:1198	the relative mRNA expression of senescence-associated gene p53	1171:1232	In addition, APF, APS, and APRS significantly downregulated the relative mRNA expression of senescence-associated gene p53 and upregulated the expression of anti-aging gene Sirt1.					
36235583	7	69	theme	APRS	933:936	arg1	order					900:904	the order	896:904	the order of ameliorative effect	896:927	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	7	69	theme	APRS	933:936	arg1	APF					946:948	APRS > APS > APF	933:948	APRS > APS > APF	933:948	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	1	70	theme	pinnata	212:218	arg1	starch					220:225	Arenga pinnata starch	205:225	Arenga pinnata starch (APS)	205:231	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	1	70	theme	pinnata	212:218	arg1	APS					228:230	APS	228:230	APS	228:230	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	0	71	theme	Intestinal	71:80	arg1	Inflammation					82:93	Intestinal Inflammation	71:93	Intestinal Inflammation in Aged Mice	71:106	Arenga pinnata Resistant Starch Modulate Gut Microbiota and Ameliorate Intestinal Inflammation in Aged Mice.					
36235583	7	72	theme	>	938:938	arg1	order					900:904	the order	896:904	the order of ameliorative effect	896:927	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	7	72	theme	>	938:938	arg1	APF					946:948	APRS > APS > APF	933:948	APRS > APS > APF	933:948	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	3	73	theme	greatest	470:477	arg1	increase					479:486	the greatest increase	466:486	the greatest increase in feces of aged mice fed APF	466:516	Bifidobacterium showed the greatest increase in feces of aged mice fed APF.					
36235583	1	74	theme	starch	220:225	arg1	effects					152:158	the regulatory effects	137:158	the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice	137:343	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	2	75	theme	microbiota	377:386	arg1	composition					388:398	gut microbiota composition	373:398	gut microbiota composition	373:398	APF, APS, and APRS altered gut microbiota composition and exhibited different prebiotic effects.					
36235583	1	76	theme	gut	274:276	arg1	microbiota					278:287	gut microbiota	274:287	gut microbiota	274:287	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	7	77	theme	effect	922:927	arg1	order					900:904	the order	896:904	the order of ameliorative effect	896:927	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	7	77	theme	effect	922:927	arg1	APF					946:948	APRS > APS > APF	933:948	APRS > APS > APF	933:948	APF, APS, and APRS treatments improved intestinal inflammation in aged mice and the order of ameliorative effect was APRS > APS > APF.					
36235583	1	78	theme	regulatory	141:150	arg1	effects					152:158	the regulatory effects	137:158	the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice	137:343	This study aimed to compare the regulatory effects of Arenga pinnata retrograded starch (APRS), Arenga pinnata starch (APS), and whole Arenga pinnata flour (APF) on gut microbiota and improvement of intestinal inflammation in aged mice.					
36235583	8	79	theme	cytokines	1092:1100	arg1	expression					994:1003	relative mRNA expression	980:1003	relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10)	980:1108	APRS significantly decreased relative mRNA expression of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increased anti-inflammatory cytokines (IL-10).					
36938958	9	0	theme	community	1339:1347	arg1	composition					1349:1359	community composition	1339:1359	community composition	1339:1359	In contrast, community composition was dependent on fHMO structure.					
36938958	4	1	theme	in	723:724	arg1	microbiome					740:749	an in vitro modeled microbiome	720:749	an in vitro modeled microbiome supplemented by B. infantis and/or E. hallii	720:794	This was also conducted in an in vitro modeled microbiome supplemented by B. infantis and/or E. hallii.					
36938958	10	2	theme	chain	1419:1423	arg1	acid					1431:1434	short chain fatty acid	1413:1434	short chain fatty acid production	1413:1445	CONCLUSION Whereas short chain fatty acid production is not significantly altered by the specific fHMO structure introduced to the modeled community, fHMOs influences the composition of the gut microbiome.					
36938958	1	3	theme	human	189:193	arg1	fHMOs					218:222	fHMOs	218:222	fHMOs	218:222	SCOPE Fucosylated human milk oligosaccharides (fHMOs) are metabolized by Bifidobacterium infantis and promote syntrophic interactions between microbiota that colonize the infant gut.					
36938958	1	3	theme	human	189:193	arg1	oligosaccharides					200:215	SCOPE Fucosylated human milk oligosaccharides	171:215	SCOPE Fucosylated human milk oligosaccharides (fHMOs)	171:223	SCOPE Fucosylated human milk oligosaccharides (fHMOs) are metabolized by Bifidobacterium infantis and promote syntrophic interactions between microbiota that colonize the infant gut.					
36938958	1	4	theme	milk	195:198	arg1	fHMOs					218:222	fHMOs	218:222	fHMOs	218:222	SCOPE Fucosylated human milk oligosaccharides (fHMOs) are metabolized by Bifidobacterium infantis and promote syntrophic interactions between microbiota that colonize the infant gut.					
36938958	1	4	theme	milk	195:198	arg1	oligosaccharides					200:215	SCOPE Fucosylated human milk oligosaccharides	171:215	SCOPE Fucosylated human milk oligosaccharides (fHMOs)	171:223	SCOPE Fucosylated human milk oligosaccharides (fHMOs) are metabolized by Bifidobacterium infantis and promote syntrophic interactions between microbiota that colonize the infant gut.					
36938958	6	5	theme	community	948:956	arg1	composition					958:968	community composition	948:968	community composition	948:968	Total B. infantis and E. hallii populations were quantified through qRT-PCR and community composition through 16S amplicon sequencing.					
36938958	4	6	dep	in	723:724	arg1	vitro					726:730	vitro	726:730	vitro	726:730	This was also conducted in an in vitro modeled microbiome supplemented by B. infantis and/or E. hallii.					
36938958	2	7	from	role	358:361	arg1	function					427:434	net microbiome function	412:434	net microbiome function	412:434	The role of fHMO structure on syntrophic interactions and net microbiome function is not yet fully understood.					
36938958	2	7	from	role	358:361	arg1	interactions					395:406	syntrophic interactions	384:406	syntrophic interactions	384:406	The role of fHMO structure on syntrophic interactions and net microbiome function is not yet fully understood.					
36938958	3	8	theme	co-culture	563:572	arg1	fermentations					574:586	mono- and co-culture fermentations	553:586	mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL)	553:640	METHODS AND RESULTS Metabolite production and microbial populations were tracked during mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL) by two B. infantis strains and Eubacterium hallii.					
36938958	0	9	theme	modeled	140:146	arg1	microbiome					159:168	a modeled infant gut microbiome	138:168	a modeled infant gut microbiome	138:168	Fucosylated human milk oligosaccharides drive structure-specific syntrophy between Bifidobacterium infantis and Eubacterium hallii within a modeled infant gut microbiome.					
36938958	2	10	theme	syntrophic	384:393	arg1	interactions					395:406	syntrophic interactions	384:406	syntrophic interactions	384:406	The role of fHMO structure on syntrophic interactions and net microbiome function is not yet fully understood.					
36938958	5	11	theme	performance	833:843	arg1	chromatography					852:865	high performance liquid chromatography	828:865	high performance liquid chromatography	828:865	Metabolites were quantified by high performance liquid chromatography.					
36938958	8	12	theme	modeled	1307:1313	arg1	community					1315:1323	the modeled community	1303:1323	the modeled community	1303:1323	Within the modeled microbial community, fHMO structure did not strongly alter metabolite production in aggregate, potentially due to functional redundancy within the modeled community.					
36938958	3	13	theme	Metabolite	485:494	arg1	production					496:505	Metabolite production	485:505	Metabolite production	485:505	METHODS AND RESULTS Metabolite production and microbial populations were tracked during mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL) by two B. infantis strains and Eubacterium hallii.					
36938958	9	14	theme	fHMO	1378:1381	arg1	structure					1383:1391	fHMO structure	1378:1391	fHMO structure	1378:1391	In contrast, community composition was dependent on fHMO structure.					
36938958	8	15	theme	due	1267:1269	arg1	production					1230:1239	metabolite production	1219:1239	metabolite production	1219:1239	Within the modeled microbial community, fHMO structure did not strongly alter metabolite production in aggregate, potentially due to functional redundancy within the modeled community.					
36938958	10	16	theme	fHMO	1492:1495	arg1	structure					1497:1505	the specific fHMO structure	1479:1505	the specific fHMO structure introduced to the modeled community, fHMOs	1479:1548	CONCLUSION Whereas short chain fatty acid production is not significantly altered by the specific fHMO structure introduced to the modeled community, fHMOs influences the composition of the gut microbiome.					
36938958	3	17	theme	fucosyllactose	593:606	arg1	fermentations					574:586	mono- and co-culture fermentations	553:586	mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL)	553:640	METHODS AND RESULTS Metabolite production and microbial populations were tracked during mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL) by two B. infantis strains and Eubacterium hallii.					
36938958	8	18	theme	metabolite	1219:1228	arg1	production					1230:1239	metabolite production	1219:1239	metabolite production	1219:1239	Within the modeled microbial community, fHMO structure did not strongly alter metabolite production in aggregate, potentially due to functional redundancy within the modeled community.					
36938958	0	19	theme	human	12:16	arg1	oligosaccharides					23:38	Fucosylated human milk oligosaccharides	0:38	Fucosylated human milk oligosaccharides	0:38	Fucosylated human milk oligosaccharides drive structure-specific syntrophy between Bifidobacterium infantis and Eubacterium hallii within a modeled infant gut microbiome.					
36938958	7	20	theme	Differential	1003:1014	arg1	metabolism					1016:1025	Differential metabolism	1003:1025	Differential metabolism of 2'FL and DFL by B. infantis strains	1003:1064	Differential metabolism of 2'FL and DFL by B. infantis strains gave rise to strain- and fHMO structure-specific syntrophy with E. hallii.					
36938958	7	21	with	strain-	1079:1085	arg1	hallii					1133:1138	E. hallii	1130:1138	E. hallii	1130:1138	Differential metabolism of 2'FL and DFL by B. infantis strains gave rise to strain- and fHMO structure-specific syntrophy with E. hallii.					
36938958	0	22	theme	Fucosylated	0:10	arg1	oligosaccharides					23:38	Fucosylated human milk oligosaccharides	0:38	Fucosylated human milk oligosaccharides	0:38	Fucosylated human milk oligosaccharides drive structure-specific syntrophy between Bifidobacterium infantis and Eubacterium hallii within a modeled infant gut microbiome.					
36938958	3	23	dep	METHODS	465:471	arg1	production					496:505	Metabolite production	485:505	Metabolite production	485:505	METHODS AND RESULTS Metabolite production and microbial populations were tracked during mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL) by two B. infantis strains and Eubacterium hallii.					
36938958	3	23	dep	METHODS	465:471	arg1	populations					521:531	microbial populations	511:531	microbial populations	511:531	METHODS AND RESULTS Metabolite production and microbial populations were tracked during mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL) by two B. infantis strains and Eubacterium hallii.					
36938958	9	24	from	dependent	1365:1373	arg1	contrast					1329:1336	contrast	1329:1336	contrast	1329:1336	In contrast, community composition was dependent on fHMO structure.					
36938958	5	25	theme	liquid	845:850	arg1	chromatography					852:865	high performance liquid chromatography	828:865	high performance liquid chromatography	828:865	Metabolites were quantified by high performance liquid chromatography.					
36938958	0	26	theme	gut	155:157	arg1	microbiome					159:168	a modeled infant gut microbiome	138:168	a modeled infant gut microbiome	138:168	Fucosylated human milk oligosaccharides drive structure-specific syntrophy between Bifidobacterium infantis and Eubacterium hallii within a modeled infant gut microbiome.					
36938958	10	27	theme	modeled	1525:1531	arg1	community					1533:1541	the modeled community	1521:1541	the modeled community	1521:1541	CONCLUSION Whereas short chain fatty acid production is not significantly altered by the specific fHMO structure introduced to the modeled community, fHMOs influences the composition of the gut microbiome.					
36938958	10	27	theme	modeled	1525:1531	arg1	fHMOs					1544:1548	fHMOs	1544:1548	fHMOs	1544:1548	CONCLUSION Whereas short chain fatty acid production is not significantly altered by the specific fHMO structure introduced to the modeled community, fHMOs influences the composition of the gut microbiome.					
36938958	0	28	theme	milk	18:21	arg1	oligosaccharides					23:38	Fucosylated human milk oligosaccharides	0:38	Fucosylated human milk oligosaccharides	0:38	Fucosylated human milk oligosaccharides drive structure-specific syntrophy between Bifidobacterium infantis and Eubacterium hallii within a modeled infant gut microbiome.					
36938958	1	29	theme	infant	342:347	arg1	gut					349:351	the infant gut	338:351	the infant gut	338:351	SCOPE Fucosylated human milk oligosaccharides (fHMOs) are metabolized by Bifidobacterium infantis and promote syntrophic interactions between microbiota that colonize the infant gut.					
36938958	0	30	theme	infant	148:153	arg1	microbiome					159:168	a modeled infant gut microbiome	138:168	a modeled infant gut microbiome	138:168	Fucosylated human milk oligosaccharides drive structure-specific syntrophy between Bifidobacterium infantis and Eubacterium hallii within a modeled infant gut microbiome.					
36938958	8	31	from	production	1230:1239	arg1	aggregate					1244:1252	aggregate	1244:1252	aggregate	1244:1252	Within the modeled microbial community, fHMO structure did not strongly alter metabolite production in aggregate, potentially due to functional redundancy within the modeled community.					
36938958	1	32	theme	SCOPE	171:175	arg1	fHMOs					218:222	fHMOs	218:222	fHMOs	218:222	SCOPE Fucosylated human milk oligosaccharides (fHMOs) are metabolized by Bifidobacterium infantis and promote syntrophic interactions between microbiota that colonize the infant gut.					
36938958	1	32	theme	SCOPE	171:175	arg1	oligosaccharides					200:215	SCOPE Fucosylated human milk oligosaccharides	171:215	SCOPE Fucosylated human milk oligosaccharides (fHMOs)	171:223	SCOPE Fucosylated human milk oligosaccharides (fHMOs) are metabolized by Bifidobacterium infantis and promote syntrophic interactions between microbiota that colonize the infant gut.					
36938958	0	33	theme	structure-specific	46:63	arg1	syntrophy					65:73	structure-specific syntrophy	46:73	structure-specific syntrophy between Bifidobacterium infantis and Eubacterium hallii	46:129	Fucosylated human milk oligosaccharides drive structure-specific syntrophy between Bifidobacterium infantis and Eubacterium hallii within a modeled infant gut microbiome.					
36938958	7	34	theme	fHMO	1091:1094	arg1	syntrophy					1115:1123	fHMO structure-specific syntrophy	1091:1123	fHMO structure-specific syntrophy	1091:1123	Differential metabolism of 2'FL and DFL by B. infantis strains gave rise to strain- and fHMO structure-specific syntrophy with E. hallii.					
36938958	7	35	with	syntrophy	1115:1123	arg1	hallii					1133:1138	E. hallii	1130:1138	E. hallii	1130:1138	Differential metabolism of 2'FL and DFL by B. infantis strains gave rise to strain- and fHMO structure-specific syntrophy with E. hallii.					
36938958	3	36	dep	B.	649:650	arg1	infantis					652:659	infantis	652:659	infantis	652:659	METHODS AND RESULTS Metabolite production and microbial populations were tracked during mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL) by two B. infantis strains and Eubacterium hallii.					
36938958	7	37	theme	structure-specific	1096:1113	arg1	syntrophy					1115:1123	fHMO structure-specific syntrophy	1091:1123	fHMO structure-specific syntrophy	1091:1123	Differential metabolism of 2'FL and DFL by B. infantis strains gave rise to strain- and fHMO structure-specific syntrophy with E. hallii.					
36938958	6	38	theme	hallii	893:898	arg1	populations					900:910	Total B. infantis and E. hallii populations	868:910	Total B. infantis and E. hallii populations	868:910	Total B. infantis and E. hallii populations were quantified through qRT-PCR and community composition through 16S amplicon sequencing.					
36938958	10	39	theme	short	1413:1417	arg1	acid					1431:1434	short chain fatty acid	1413:1434	short chain fatty acid production	1413:1445	CONCLUSION Whereas short chain fatty acid production is not significantly altered by the specific fHMO structure introduced to the modeled community, fHMOs influences the composition of the gut microbiome.					
36938958	1	40	theme	Bifidobacterium	244:258	arg1	infantis					260:267	Bifidobacterium infantis	244:267	Bifidobacterium infantis	244:267	SCOPE Fucosylated human milk oligosaccharides (fHMOs) are metabolized by Bifidobacterium infantis and promote syntrophic interactions between microbiota that colonize the infant gut.					
36938958	8	41	theme	functional	1274:1283	arg1	redundancy					1285:1294	functional redundancy	1274:1294	functional redundancy within the modeled community	1274:1323	Within the modeled microbial community, fHMO structure did not strongly alter metabolite production in aggregate, potentially due to functional redundancy within the modeled community.					
36938958	8	42	theme	modeled	1152:1158	arg1	community					1170:1178	the modeled microbial community	1148:1178	the modeled microbial community	1148:1178	Within the modeled microbial community, fHMO structure did not strongly alter metabolite production in aggregate, potentially due to functional redundancy within the modeled community.					
36938958	3	43	theme	difucosyllactose	619:634	arg1	fermentations					574:586	mono- and co-culture fermentations	553:586	mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL)	553:640	METHODS AND RESULTS Metabolite production and microbial populations were tracked during mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL) by two B. infantis strains and Eubacterium hallii.					
36938958	10	44	theme	gut	1584:1586	arg1	microbiome					1588:1597	the gut microbiome	1580:1597	the gut microbiome	1580:1597	CONCLUSION Whereas short chain fatty acid production is not significantly altered by the specific fHMO structure introduced to the modeled community, fHMOs influences the composition of the gut microbiome.					
36938958	3	45	theme	mono-	553:557	arg1	fermentations					574:586	mono- and co-culture fermentations	553:586	mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL)	553:640	METHODS AND RESULTS Metabolite production and microbial populations were tracked during mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL) by two B. infantis strains and Eubacterium hallii.					
36938958	5	46	theme	high	828:831	arg1	chromatography					852:865	high performance liquid chromatography	828:865	high performance liquid chromatography	828:865	Metabolites were quantified by high performance liquid chromatography.					
36938958	10	47	theme	microbiome	1588:1597	arg1	composition					1565:1575	the composition	1561:1575	the composition of the gut microbiome	1561:1597	CONCLUSION Whereas short chain fatty acid production is not significantly altered by the specific fHMO structure introduced to the modeled community, fHMOs influences the composition of the gut microbiome.					
36938958	1	48	theme	Fucosylated	177:187	arg1	fHMOs					218:222	fHMOs	218:222	fHMOs	218:222	SCOPE Fucosylated human milk oligosaccharides (fHMOs) are metabolized by Bifidobacterium infantis and promote syntrophic interactions between microbiota that colonize the infant gut.					
36938958	1	48	theme	Fucosylated	177:187	arg1	oligosaccharides					200:215	SCOPE Fucosylated human milk oligosaccharides	171:215	SCOPE Fucosylated human milk oligosaccharides (fHMOs)	171:223	SCOPE Fucosylated human milk oligosaccharides (fHMOs) are metabolized by Bifidobacterium infantis and promote syntrophic interactions between microbiota that colonize the infant gut.					
36938958	7	49	theme	B.	1046:1047	arg1	strains					1058:1064	B. infantis strains	1046:1064	B. infantis strains	1046:1064	Differential metabolism of 2'FL and DFL by B. infantis strains gave rise to strain- and fHMO structure-specific syntrophy with E. hallii.					
36938958	6	50	theme	amplicon	982:989	arg1	sequencing					991:1000	amplicon sequencing	982:1000	amplicon sequencing	982:1000	Total B. infantis and E. hallii populations were quantified through qRT-PCR and community composition through 16S amplicon sequencing.					
36938958	4	51	theme	modeled	732:738	arg1	microbiome					740:749	an in vitro modeled microbiome	720:749	an in vitro modeled microbiome supplemented by B. infantis and/or E. hallii	720:794	This was also conducted in an in vitro modeled microbiome supplemented by B. infantis and/or E. hallii.					
36938958	6	52	theme	infantis	877:884	arg1	populations					900:910	Total B. infantis and E. hallii populations	868:910	Total B. infantis and E. hallii populations	868:910	Total B. infantis and E. hallii populations were quantified through qRT-PCR and community composition through 16S amplicon sequencing.					
36938958	3	53	theme	B.	649:650	arg1	strains					661:667	two B. infantis strains	645:667	two B. infantis strains	645:667	METHODS AND RESULTS Metabolite production and microbial populations were tracked during mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL) by two B. infantis strains and Eubacterium hallii.					
36938958	7	54	theme	infantis	1049:1056	arg1	strains					1058:1064	B. infantis strains	1046:1064	B. infantis strains	1046:1064	Differential metabolism of 2'FL and DFL by B. infantis strains gave rise to strain- and fHMO structure-specific syntrophy with E. hallii.					
36938958	2	55	theme	structure	371:379	arg1	role					358:361	The role	354:361	The role of fHMO structure on syntrophic interactions and net microbiome function	354:434	The role of fHMO structure on syntrophic interactions and net microbiome function is not yet fully understood.					
36938958	10	56	theme	fatty	1425:1429	arg1	acid					1431:1434	short chain fatty acid	1413:1434	short chain fatty acid production	1413:1445	CONCLUSION Whereas short chain fatty acid production is not significantly altered by the specific fHMO structure introduced to the modeled community, fHMOs influences the composition of the gut microbiome.					
36938958	8	57	theme	microbial	1160:1168	arg1	community					1170:1178	the modeled microbial community	1148:1178	the modeled microbial community	1148:1178	Within the modeled microbial community, fHMO structure did not strongly alter metabolite production in aggregate, potentially due to functional redundancy within the modeled community.					
36938958	7	58	theme	DFL	1039:1041	arg1	metabolism					1016:1025	Differential metabolism	1003:1025	Differential metabolism of 2'FL and DFL by B. infantis strains	1003:1064	Differential metabolism of 2'FL and DFL by B. infantis strains gave rise to strain- and fHMO structure-specific syntrophy with E. hallii.					
36938958	2	59	theme	fHMO	366:369	arg1	structure					371:379	fHMO structure	366:379	fHMO structure	366:379	The role of fHMO structure on syntrophic interactions and net microbiome function is not yet fully understood.					
36938958	8	60	theme	fHMO	1181:1184	arg1	structure					1186:1194	fHMO structure	1181:1194	fHMO structure	1181:1194	Within the modeled microbial community, fHMO structure did not strongly alter metabolite production in aggregate, potentially due to functional redundancy within the modeled community.					
36938958	3	61	theme	microbial	511:519	arg1	populations					521:531	microbial populations	511:531	microbial populations	511:531	METHODS AND RESULTS Metabolite production and microbial populations were tracked during mono- and co-culture fermentations of 2'fucosyllactose (2'FL) and difucosyllactose (DFL) by two B. infantis strains and Eubacterium hallii.					
36938958	10	62	theme	acid	1431:1434	arg1	production					1436:1445	short chain fatty acid production	1413:1445	short chain fatty acid production	1413:1445	CONCLUSION Whereas short chain fatty acid production is not significantly altered by the specific fHMO structure introduced to the modeled community, fHMOs influences the composition of the gut microbiome.					
36938958	2	63	theme	microbiome	416:425	arg1	function					427:434	net microbiome function	412:434	net microbiome function	412:434	The role of fHMO structure on syntrophic interactions and net microbiome function is not yet fully understood.					
36938958	6	64	theme	Total	868:872	arg1	populations					900:910	Total B. infantis and E. hallii populations	868:910	Total B. infantis and E. hallii populations	868:910	Total B. infantis and E. hallii populations were quantified through qRT-PCR and community composition through 16S amplicon sequencing.					
36938958	9	65	from	contrast	1329:1336	arg1	dependent					1365:1373	dependent	1365:1373	dependent	1365:1373	In contrast, community composition was dependent on fHMO structure.					
36938958	10	66	theme	specific	1483:1490	arg1	structure					1497:1505	the specific fHMO structure	1479:1505	the specific fHMO structure introduced to the modeled community, fHMOs	1479:1548	CONCLUSION Whereas short chain fatty acid production is not significantly altered by the specific fHMO structure introduced to the modeled community, fHMOs influences the composition of the gut microbiome.					
36938958	7	67	theme	FL	1032:1033	arg1	metabolism					1016:1025	Differential metabolism	1003:1025	Differential metabolism of 2'FL and DFL by B. infantis strains	1003:1064	Differential metabolism of 2'FL and DFL by B. infantis strains gave rise to strain- and fHMO structure-specific syntrophy with E. hallii.					
36938958	2	68	theme	net	412:414	arg1	function					427:434	net microbiome function	412:434	net microbiome function	412:434	The role of fHMO structure on syntrophic interactions and net microbiome function is not yet fully understood.					
36938958	1	69	theme	syntrophic	281:290	arg1	interactions					292:303	syntrophic interactions	281:303	syntrophic interactions between microbiota that colonize the infant gut	281:351	SCOPE Fucosylated human milk oligosaccharides (fHMOs) are metabolized by Bifidobacterium infantis and promote syntrophic interactions between microbiota that colonize the infant gut.					
36346259	2	0	theme	Lut	400:402	arg1	effects					383:389	the effects	379:389	the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film	379:476	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	10	1	theme	surface	1369:1375	arg1	cracks					1377:1382	the surface cracks	1365:1382	the surface cracks of mucilage film	1365:1399	Maltodextrin filled the surface cracks, but lutein increased the surface cracks of mucilage film.					
36346259	2	2	from	effects	383:389	arg1	properties					440:449	the physical and chemical properties	414:449	the physical and chemical properties of the mucilage based film	414:476	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	0	3	theme	lutein	63:68	arg1	nanoparticles					96:108	lutein, maltodextrin and alumina nanoparticles	63:108	lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release	63:166	Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release.					
36346259	11	4	theme	crystalline	1519:1529	arg1	properties					1531:1540	crystalline properties	1519:1540	crystalline properties	1519:1540	The amorphous structure of the pure cress seed mucilage film was confirmed by XRD, which the alumina and lutein gave crystalline properties in the film.					
36346259	7	5	theme	antioxidant	999:1009	arg1	properties					1011:1020	its antioxidant properties	995:1020	its antioxidant properties	995:1020	Lutein greatly increased its antioxidant properties, but alumina slightly increased its antioxidant properties.					
36346259	11	6	theme	film	1458:1461	arg1	structure					1416:1424	The amorphous structure	1402:1424	The amorphous structure of the pure cress seed mucilage film	1402:1461	The amorphous structure of the pure cress seed mucilage film was confirmed by XRD, which the alumina and lutein gave crystalline properties in the film.					
36346259	13	7	theme	release	1768:1774	arg1	rate					1776:1779	the release rate	1764:1779	the release rate	1764:1779	The release results showed that the release rate of lutein depends on the structure of the film and by changing the structure of the film, the release rate can be purposefully controlled according to the required release rate.					
36346259	13	8	theme	lutein	1677:1682	arg1	rate					1669:1672	the release rate	1657:1672	the release rate of lutein	1657:1682	The release results showed that the release rate of lutein depends on the structure of the film and by changing the structure of the film, the release rate can be purposefully controlled according to the required release rate.					
36346259	1	9	theme	cress	291:295	arg1	mucilage					302:309	cress seed mucilage	291:309	cress seed mucilage (Muc/MDex/Lut/Al)	291:327	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	1	9	theme	cress	291:295	arg1	Muc/MDex/Lut/Al					312:326	Muc/MDex/Lut/Al	312:326	Muc/MDex/Lut/Al	312:326	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	0	10	dep	nanoparticles	96:108	arg1	properties					127:136	Physicochemical properties	111:136	Physicochemical properties	111:136	Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release.					
36346259	0	10	dep	nanoparticles	96:108	arg1	release					160:166	lutein controlled release	142:166	lutein controlled release	142:166	Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release.					
36346259	5	11	theme	film	861:864	arg1	solubility					822:831	solubility	822:831	solubility	822:831	The results showed that lutein, alumina and maltodextrin increased the film thickness and lutein decreased the solubility and moisture content of the film.					
36346259	5	11	theme	film	861:864	arg1	content					846:852	moisture content	837:852	moisture content	837:852	The results showed that lutein, alumina and maltodextrin increased the film thickness and lutein decreased the solubility and moisture content of the film.					
36346259	0	12	theme	maltodextrin	71:82	arg1	nanoparticles					96:108	lutein, maltodextrin and alumina nanoparticles	63:108	lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release	63:166	Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release.					
36346259	1	13	theme	seed	297:300	arg1	mucilage					302:309	cress seed mucilage	291:309	cress seed mucilage (Muc/MDex/Lut/Al)	291:327	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	1	13	theme	seed	297:300	arg1	Muc/MDex/Lut/Al					312:326	Muc/MDex/Lut/Al	312:326	Muc/MDex/Lut/Al	312:326	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	11	14	theme	seed	1444:1447	arg1	film					1458:1461	the pure cress seed mucilage film	1429:1461	the pure cress seed mucilage film	1429:1461	The amorphous structure of the pure cress seed mucilage film was confirmed by XRD, which the alumina and lutein gave crystalline properties in the film.					
36346259	5	15	theme	moisture	837:844	arg1	content					846:852	moisture content	837:852	moisture content	837:852	The results showed that lutein, alumina and maltodextrin increased the film thickness and lutein decreased the solubility and moisture content of the film.					
36346259	13	16	theme	film	1758:1761	arg1	structure					1741:1749	the structure	1737:1749	the structure of the film	1737:1761	The release results showed that the release rate of lutein depends on the structure of the film and by changing the structure of the film, the release rate can be purposefully controlled according to the required release rate.					
36346259	2	17	used	used	365:368	arg2	CCD					356:358	CCD	356:358	CCD	356:358	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	2	17	used	used	365:368	arg2	design					348:353	Central composite design	330:353	Central composite design (CCD)	330:359	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	11	18	theme	cress	1438:1442	arg1	film					1458:1461	the pure cress seed mucilage film	1429:1461	the pure cress seed mucilage film	1429:1461	The amorphous structure of the pure cress seed mucilage film was confirmed by XRD, which the alumina and lutein gave crystalline properties in the film.					
36346259	4	19	theme	lutein	651:656	arg1	release					640:646	The release	636:646	The release of lutein from the film	636:670	The release of lutein from the film was investigated at 25 °C for 15 days.					
36346259	2	20	theme	composite	338:346	arg1	CCD					356:358	CCD	356:358	CCD	356:358	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	2	20	theme	composite	338:346	arg1	design					348:353	Central composite design	330:353	Central composite design (CCD)	330:359	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	0	21	theme	alumina	88:94	arg1	nanoparticles					96:108	lutein, maltodextrin and alumina nanoparticles	63:108	lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release	63:166	Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release.					
36346259	6	22	dep	film	922:925	arg1	the					918:920	the	918:920	the	918:920	Maltodextrin improved the mechanical properties of the film and lutein reduced the film's flexibility.					
36346259	6	22	dep	film	922:925	arg1	flexibility					957:967	reduced the film's flexibility	938:967	reduced the film's flexibility	938:967	Maltodextrin improved the mechanical properties of the film and lutein reduced the film's flexibility.					
36346259	2	23	theme	MDex	394:397	arg1	effects					383:389	the effects	379:389	the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film	379:476	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	6	24	theme	mechanical	893:902	arg1	properties					904:913	the mechanical properties	889:913	the mechanical properties of the film and lutein reduced the film's flexibility	889:967	Maltodextrin improved the mechanical properties of the film and lutein reduced the film's flexibility.					
36346259	2	25	theme	Central	330:336	arg1	CCD					356:358	CCD	356:358	CCD	356:358	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	2	25	theme	Central	330:336	arg1	design					348:353	Central composite design	330:353	Central composite design (CCD)	330:359	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	13	26	theme	film	1716:1719	arg1	structure					1699:1707	the structure	1695:1707	the structure of the film	1695:1719	The release results showed that the release rate of lutein depends on the structure of the film and by changing the structure of the film, the release rate can be purposefully controlled according to the required release rate.					
36346259	2	27	theme	film	473:476	arg1	properties					440:449	the physical and chemical properties	414:449	the physical and chemical properties of the mucilage based film	414:476	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	6	28	theme	reduced	938:944	arg1	flexibility					957:967	reduced the film's flexibility	938:967	reduced the film's flexibility	938:967	Maltodextrin improved the mechanical properties of the film and lutein reduced the film's flexibility.					
36346259	0	29	theme	Biodegradable	0:12	arg1	film					14:17	Biodegradable film	0:17	Biodegradable film	0:17	Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release.					
36346259	8	30	theme	antibacterial	1128:1140	arg1	properties					1142:1151	the antibacterial properties	1124:1151	the antibacterial properties of the film	1124:1163	Lutein, alumina and maltodextrin improved the antibacterial properties of the film.					
36346259	13	31	theme	release	1629:1635	arg1	results					1637:1643	The release results	1625:1643	The release results	1625:1643	The release results showed that the release rate of lutein depends on the structure of the film and by changing the structure of the film, the release rate can be purposefully controlled according to the required release rate.					
36346259	13	32	theme	release	1661:1667	arg1	rate					1669:1672	the release rate	1657:1672	the release rate of lutein	1657:1682	The release results showed that the release rate of lutein depends on the structure of the film and by changing the structure of the film, the release rate can be purposefully controlled according to the required release rate.					
36346259	6	33	theme	lutein	931:936	arg1	properties					904:913	the mechanical properties	889:913	the mechanical properties of the film and lutein reduced the film's flexibility	889:967	Maltodextrin improved the mechanical properties of the film and lutein reduced the film's flexibility.					
36346259	0	34	theme	Physicochemical	111:125	arg1	properties					127:136	Physicochemical properties	111:136	Physicochemical properties	111:136	Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release.					
36346259	3	35	theme	physicochemical	483:497	arg1	properties					541:550	The physicochemical, mechanical, antimicrobial and structural properties	479:550	The physicochemical, mechanical, antimicrobial and structural properties of the films	479:563	The physicochemical, mechanical, antimicrobial and structural properties of the films were studied by various techniques such as FTIR, SEM, and XRD and TGA.					
36346259	13	36	theme	release	1838:1844	arg1	rate					1846:1849	the required release rate	1825:1849	the required release rate	1825:1849	The release results showed that the release rate of lutein depends on the structure of the film and by changing the structure of the film, the release rate can be purposefully controlled according to the required release rate.					
36346259	8	37	theme	film	1160:1163	arg1	properties					1142:1151	the antibacterial properties	1124:1151	the antibacterial properties of the film	1124:1163	Lutein, alumina and maltodextrin improved the antibacterial properties of the film.					
36346259	6	38	theme	film	922:925	arg1	properties					904:913	the mechanical properties	889:913	the mechanical properties of the film and lutein reduced the film's flexibility	889:967	Maltodextrin improved the mechanical properties of the film and lutein reduced the film's flexibility.					
36346259	0	39	theme	seed	34:37	arg1	mucilage					39:46	cress seed mucilage	28:46	cress seed mucilage	28:46	Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release.					
36346259	9	40	theme	non-growth	1219:1228	arg1	halo					1230:1233	26 ± 0.5 and 23 ± 0.8 mm non-growth halo	1194:1233	26 ± 0.5 and 23 ± 0.8 mm non-growth halo against to Staphylococcus aureus and Escherichia coli	1194:1287	Muc/MDex/Lut/Al film showed 26 ± 0.5 and 23 ± 0.8 mm non-growth halo against to Staphylococcus aureus and Escherichia coli, respectively.					
36346259	3	41	theme	films	559:563	arg1	properties					541:550	The physicochemical, mechanical, antimicrobial and structural properties	479:550	The physicochemical, mechanical, antimicrobial and structural properties of the films	479:563	The physicochemical, mechanical, antimicrobial and structural properties of the films were studied by various techniques such as FTIR, SEM, and XRD and TGA.					
36346259	12	42	theme	thermal	1594:1600	arg1	stability					1602:1610	the thermal stability	1590:1610	the thermal stability of the film	1590:1622	Maltodextrin and alumina increased the thermal stability of the film.					
36346259	0	43	theme	cress	28:32	arg1	mucilage					39:46	cress seed mucilage	28:46	cress seed mucilage	28:46	Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release.					
36346259	2	44	theme	chemical	431:438	arg1	properties					440:449	the physical and chemical properties	414:449	the physical and chemical properties of the mucilage based film	414:476	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	3	45	theme	mechanical	500:509	arg1	properties					541:550	The physicochemical, mechanical, antimicrobial and structural properties	479:550	The physicochemical, mechanical, antimicrobial and structural properties of the films	479:563	The physicochemical, mechanical, antimicrobial and structural properties of the films were studied by various techniques such as FTIR, SEM, and XRD and TGA.					
36346259	10	46	theme	mucilage	1387:1394	arg1	cracks					1377:1382	the surface cracks	1365:1382	the surface cracks of mucilage film	1365:1399	Maltodextrin filled the surface cracks, but lutein increased the surface cracks of mucilage film.					
36346259	0	47	theme	controlled	149:158	arg1	release					160:166	lutein controlled release	142:166	lutein controlled release	142:166	Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release.					
36346259	3	48	theme	structural	530:539	arg1	properties					541:550	The physicochemical, mechanical, antimicrobial and structural properties	479:550	The physicochemical, mechanical, antimicrobial and structural properties of the films	479:563	The physicochemical, mechanical, antimicrobial and structural properties of the films were studied by various techniques such as FTIR, SEM, and XRD and TGA.					
36346259	1	49	used	used	248:251	arg2	alumina					230:236	alumina	230:236	alumina (Al)	230:241	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	1	49	used	used	248:251	arg2	Lut					221:223	Lut	221:223	Lut	221:223	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	1	49	used	used	248:251	arg2	MDex					198:201	MDex	198:201	MDex	198:201	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	1	49	used	used	248:251	arg2	maltodextrin					184:195	maltodextrin	184:195	maltodextrin (MDex)	184:202	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	1	49	used	used	248:251	arg2	pigment					212:218	lutein pigment	205:218	lutein pigment (Lut)	205:224	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	0	50	theme	lutein	142:147	arg1	release					160:166	lutein controlled release	142:166	lutein controlled release	142:166	Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release.					
36346259	3	51	theme	various	581:587	arg1	TGA					631:633	TGA	631:633	TGA	631:633	The physicochemical, mechanical, antimicrobial and structural properties of the films were studied by various techniques such as FTIR, SEM, and XRD and TGA.					
36346259	3	51	theme	various	581:587	arg1	SEM					614:616	SEM	614:616	SEM	614:616	The physicochemical, mechanical, antimicrobial and structural properties of the films were studied by various techniques such as FTIR, SEM, and XRD and TGA.					
36346259	3	51	theme	various	581:587	arg1	techniques					589:598	various techniques	581:598	various techniques such as FTIR, SEM, and XRD and TGA	581:633	The physicochemical, mechanical, antimicrobial and structural properties of the films were studied by various techniques such as FTIR, SEM, and XRD and TGA.					
36346259	3	51	theme	various	581:587	arg1	FTIR					608:611	FTIR	608:611	FTIR	608:611	The physicochemical, mechanical, antimicrobial and structural properties of the films were studied by various techniques such as FTIR, SEM, and XRD and TGA.					
36346259	3	51	theme	various	581:587	arg1	XRD					623:625	XRD	623:625	XRD	623:625	The physicochemical, mechanical, antimicrobial and structural properties of the films were studied by various techniques such as FTIR, SEM, and XRD and TGA.					
36346259	13	52	theme	required	1829:1836	arg1	rate					1846:1849	the required release rate	1825:1849	the required release rate	1825:1849	The release results showed that the release rate of lutein depends on the structure of the film and by changing the structure of the film, the release rate can be purposefully controlled according to the required release rate.					
36346259	5	53	theme	film	782:785	arg1	thickness					787:795	the film thickness	778:795	the film thickness	778:795	The results showed that lutein, alumina and maltodextrin increased the film thickness and lutein decreased the solubility and moisture content of the film.					
36346259	11	54	theme	amorphous	1406:1414	arg1	structure					1416:1424	The amorphous structure	1402:1424	The amorphous structure of the pure cress seed mucilage film	1402:1461	The amorphous structure of the pure cress seed mucilage film was confirmed by XRD, which the alumina and lutein gave crystalline properties in the film.					
36346259	1	55	theme	biodegradable	263:275	arg1	film					277:280	biodegradable film	263:280	biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al)	263:327	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	2	56	theme	based	467:471	arg1	film					473:476	the mucilage based film	454:476	the mucilage based film	454:476	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	1	57	dep	alumina	230:236	arg1	Al					239:240	Al	239:240	Al	239:240	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	12	58	theme	film	1619:1622	arg1	stability					1602:1610	the thermal stability	1590:1610	the thermal stability of the film	1590:1622	Maltodextrin and alumina increased the thermal stability of the film.					
36346259	2	59	theme	mucilage	458:465	arg1	film					473:476	the mucilage based film	454:476	the mucilage based film	454:476	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	0	60	dep	based	19:23	arg1	modified					49:56	modified	49:56	modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release	49:166	Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release.					
36346259	11	61	theme	mucilage	1449:1456	arg1	film					1458:1461	the pure cress seed mucilage film	1429:1461	the pure cress seed mucilage film	1429:1461	The amorphous structure of the pure cress seed mucilage film was confirmed by XRD, which the alumina and lutein gave crystalline properties in the film.					
36346259	11	62	theme	pure	1433:1436	arg1	film					1458:1461	the pure cress seed mucilage film	1429:1461	the pure cress seed mucilage film	1429:1461	The amorphous structure of the pure cress seed mucilage film was confirmed by XRD, which the alumina and lutein gave crystalline properties in the film.					
36346259	1	63	theme	lutein	205:210	arg1	pigment					212:218	lutein pigment	205:218	lutein pigment (Lut)	205:224	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	1	63	theme	lutein	205:210	arg1	Lut					221:223	Lut	221:223	Lut	221:223	In this study, maltodextrin (MDex), lutein pigment (Lut) and alumina (Al) were used to modify biodegradable film based on cress seed mucilage (Muc/MDex/Lut/Al).					
36346259	2	64	theme	Al	408:409	arg1	effects					383:389	the effects	379:389	the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film	379:476	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36346259	9	65	theme	Muc/MDex/Lut/Al	1166:1180	arg1	film					1182:1185	Muc/MDex/Lut/Al film	1166:1185	Muc/MDex/Lut/Al film	1166:1185	Muc/MDex/Lut/Al film showed 26 ± 0.5 and 23 ± 0.8 mm non-growth halo against to Staphylococcus aureus and Escherichia coli, respectively.					
36346259	4	66	from	film	667:670	arg1	release					640:646	The release	636:646	The release of lutein from the film	636:670	The release of lutein from the film was investigated at 25 °C for 15 days.					
36346259	10	67	theme	surface	1328:1334	arg1	cracks					1336:1341	the surface cracks	1324:1341	the surface cracks	1324:1341	Maltodextrin filled the surface cracks, but lutein increased the surface cracks of mucilage film.					
36346259	3	68	theme	antimicrobial	512:524	arg1	properties					541:550	The physicochemical, mechanical, antimicrobial and structural properties	479:550	The physicochemical, mechanical, antimicrobial and structural properties of the films	479:563	The physicochemical, mechanical, antimicrobial and structural properties of the films were studied by various techniques such as FTIR, SEM, and XRD and TGA.					
36346259	5	69	dep	solubility	822:831	arg1	the					818:820	the	818:820	the	818:820	The results showed that lutein, alumina and maltodextrin increased the film thickness and lutein decreased the solubility and moisture content of the film.					
36346259	7	70	theme	antioxidant	1058:1068	arg1	properties					1070:1079	its antioxidant properties	1054:1079	its antioxidant properties	1054:1079	Lutein greatly increased its antioxidant properties, but alumina slightly increased its antioxidant properties.					
36346259	2	71	theme	physical	418:425	arg1	properties					440:449	the physical and chemical properties	414:449	the physical and chemical properties of the mucilage based film	414:476	Central composite design (CCD) was used to study the effects of MDex, Lut and Al on the physical and chemical properties of the mucilage based film.					
36577473	8	0	theme	Gentamicin	958:967	arg1	kinetics					977:984	Gentamicin release kinetics	958:984	Gentamicin release kinetics	958:984	Gentamicin release kinetics have been determined using the Korsemeyar-Peppas model, which confirms the drug release mechanism.					
36577473	5	1	theme	release	658:664	arg1	properties					666:675	the drug release properties	649:675	the drug release properties of the hydrogels	649:692	By changing the pH environment, the drug release properties of the hydrogels can be controlled.					
36577473	8	2	theme	release	969:975	arg1	kinetics					977:984	Gentamicin release kinetics	958:984	Gentamicin release kinetics	958:984	Gentamicin release kinetics have been determined using the Korsemeyar-Peppas model, which confirms the drug release mechanism.					
36577473	4	3	theme	cytocompatibility	540:556	arg1	nature					558:563	a cytocompatibility nature	538:563	a cytocompatibility nature towards healthy normal human cell line (FR2 cells)	538:614	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	7	4	theme	drug	830:833	arg1	rate					843:846	drug release rate	830:846	drug release rate for NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite	830:893	Whereas drug release rate for NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite was 78.6 % at pH = 2.2 and 97.3 % at pH = 7.4 within 4320 min.					
36577473	1	5	theme	in-situ	185:191	arg1	release					204:210	in-situ controlled release	185:210	in-situ controlled release of gentamicin	185:224	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36577473	4	6	theme	FR2	605:607	arg1	line					599:602	healthy normal human cell line	573:602	healthy normal human cell line (FR2 cells)	573:614	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	4	6	theme	FR2	605:607	arg1	cells					609:613	FR2 cells	605:613	FR2 cells	605:613	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	8	7	theme	drug	1061:1064	arg1	mechanism					1074:1082	the drug release mechanism	1057:1082	the drug release mechanism	1057:1082	Gentamicin release kinetics have been determined using the Korsemeyar-Peppas model, which confirms the drug release mechanism.					
36577473	7	8	from	pH = 2.2	909:916	arg1	%					904:904	78.6 %	899:904	78.6 % at pH = 2.2	899:916	Whereas drug release rate for NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite was 78.6 % at pH = 2.2 and 97.3 % at pH = 7.4 within 4320 min.					
36577473	7	8	from	pH = 2.2	909:916	arg1	%					927:927	97.3 %	922:927	97.3 % at pH = 7.4 within 4320 min	922:955	Whereas drug release rate for NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite was 78.6 % at pH = 2.2 and 97.3 % at pH = 7.4 within 4320 min.					
36577473	0	9	theme	Carboxymethyl	0:12	arg1	cellulose					14:22	Carboxymethyl cellulose	0:22	Carboxymethyl cellulose	0:22	Carboxymethyl cellulose based sustainable hydrogel for colon-specific delivery of gentamicin.					
36577473	7	10	theme	hydrogel	876:883	arg1	composite					885:893	NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite	852:893	NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite	852:893	Whereas drug release rate for NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite was 78.6 % at pH = 2.2 and 97.3 % at pH = 7.4 within 4320 min.					
36577473	3	11	theme	prepared	379:386	arg1	hydrogels					388:396	The prepared hydrogels	375:396	The prepared hydrogels	375:396	The prepared hydrogels were investigated by FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis.					
36577473	5	12	theme	pH	633:634	arg1	environment					636:646	the pH environment	629:646	the pH environment	629:646	By changing the pH environment, the drug release properties of the hydrogels can be controlled.					
36577473	1	13	dep	synthesis	128:136	arg1	the					124:126	the	124:126	the	124:126	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36577473	6	14	theme	cumulative	717:726	arg1	%					784:784	76.5 %	779:784	76.5 % at pH = 2.2	779:796	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel was 76.5 % at pH = 2.2 and 87.5 % at pH = 7.4.					
36577473	6	14	theme	cumulative	717:726	arg1	rate					728:731	The cumulative rate	713:731	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel	713:773	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel was 76.5 % at pH = 2.2 and 87.5 % at pH = 7.4.					
36577473	1	15	theme	controlled	193:202	arg1	release					204:210	in-situ controlled release	185:210	in-situ controlled release of gentamicin	185:224	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36577473	0	16	theme	colon-specific	55:68	arg1	delivery					70:77	colon-specific delivery	55:77	colon-specific delivery of gentamicin	55:91	Carboxymethyl cellulose based sustainable hydrogel for colon-specific delivery of gentamicin.					
36577473	4	17	theme	prepared	518:525	arg1	sample					527:532	prepared sample	518:532	prepared sample	518:532	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	8	18	theme	release	1066:1072	arg1	mechanism					1074:1082	the drug release mechanism	1057:1082	the drug release mechanism	1057:1082	Gentamicin release kinetics have been determined using the Korsemeyar-Peppas model, which confirms the drug release mechanism.					
36577473	8	19	theme	Korsemeyar-Peppas	1017:1033	arg1	model					1035:1039	the Korsemeyar-Peppas model	1013:1039	the Korsemeyar-Peppas model	1013:1039	Gentamicin release kinetics have been determined using the Korsemeyar-Peppas model, which confirms the drug release mechanism.					
36577473	2	20	theme	boron	304:308	arg1	nanosheets					318:327	boron nitride nanosheets	304:327	boron nitride nanosheets aiming to enhancement in the adsorption rate	304:372	The prepared hydrogel was then modified using boron nitride nanosheets aiming to enhancement in the adsorption rate.					
36577473	3	21	theme	cell	459:462	arg1	viability					464:472	cell viability	459:472	cell viability	459:472	The prepared hydrogels were investigated by FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis.					
36577473	6	22	from	pH = 7.4	812:819	arg1	rate					728:731	The cumulative rate	713:731	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel	713:773	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel was 76.5 % at pH = 2.2 and 87.5 % at pH = 7.4.					
36577473	6	22	from	pH = 7.4	812:819	arg1	%					807:807	87.5 %	802:807	87.5 % at pH = 7.4	802:819	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel was 76.5 % at pH = 2.2 and 87.5 % at pH = 7.4.					
36577473	6	22	from	pH = 7.4	812:819	arg1	%					784:784	76.5 %	779:784	76.5 % at pH = 2.2	779:796	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel was 76.5 % at pH = 2.2 and 87.5 % at pH = 7.4.					
36577473	1	23	theme	current	98:104	arg1	research					106:113	The current research	94:113	The current research	94:113	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36577473	7	24	theme	NaCMC-cl-DMAA/AAc-BNNSs	852:874	arg1	composite					885:893	NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite	852:893	NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite	852:893	Whereas drug release rate for NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite was 78.6 % at pH = 2.2 and 97.3 % at pH = 7.4 within 4320 min.					
36577473	3	25	theme	XRD	425:427	arg1	analysis					474:481	FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis	419:481	FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis	419:481	The prepared hydrogels were investigated by FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis.					
36577473	4	26	theme	cell	594:597	arg1	line					599:602	healthy normal human cell line	573:602	healthy normal human cell line (FR2 cells)	573:614	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	4	26	theme	cell	594:597	arg1	cells					609:613	FR2 cells	605:613	FR2 cells	605:613	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	5	27	theme	drug	653:656	arg1	properties					666:675	the drug release properties	649:675	the drug release properties of the hydrogels	649:692	By changing the pH environment, the drug release properties of the hydrogels can be controlled.					
36577473	7	28	dep	%	904:904	arg1	Whereas					822:828	Whereas	822:828	Whereas	822:828	Whereas drug release rate for NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite was 78.6 % at pH = 2.2 and 97.3 % at pH = 7.4 within 4320 min.					
36577473	7	29	theme	release	835:841	arg1	rate					843:846	drug release rate	830:846	drug release rate for NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite	830:893	Whereas drug release rate for NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite was 78.6 % at pH = 2.2 and 97.3 % at pH = 7.4 within 4320 min.					
36577473	1	30	theme	gentamicin	215:224	arg1	improvement					139:149	improvement	139:149	improvement of NaCMC-cl-DMAA/AAc hydrogel	139:179	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36577473	1	30	theme	gentamicin	215:224	arg1	synthesis					128:136	synthesis	128:136	synthesis	128:136	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36577473	1	30	theme	gentamicin	215:224	arg1	release					204:210	in-situ controlled release	185:210	in-situ controlled release of gentamicin	185:224	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36577473	0	31	theme	sustainable	30:40	arg1	hydrogel					42:49	sustainable hydrogel	30:49	sustainable hydrogel	30:49	Carboxymethyl cellulose based sustainable hydrogel for colon-specific delivery of gentamicin.					
36577473	4	32	theme	human	588:592	arg1	line					599:602	healthy normal human cell line	573:602	healthy normal human cell line (FR2 cells)	573:614	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	4	32	theme	human	588:592	arg1	cells					609:613	FR2 cells	605:613	FR2 cells	605:613	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	6	33	from	pH = 2.2	789:796	arg1	rate					728:731	The cumulative rate	713:731	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel	713:773	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel was 76.5 % at pH = 2.2 and 87.5 % at pH = 7.4.					
36577473	6	33	from	pH = 2.2	789:796	arg1	%					807:807	87.5 %	802:807	87.5 % at pH = 7.4	802:819	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel was 76.5 % at pH = 2.2 and 87.5 % at pH = 7.4.					
36577473	6	33	from	pH = 2.2	789:796	arg1	%					784:784	76.5 %	779:784	76.5 % at pH = 2.2	779:796	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel was 76.5 % at pH = 2.2 and 87.5 % at pH = 7.4.					
36577473	3	34	theme	FTIR	419:422	arg1	analysis					474:481	FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis	419:481	FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis	419:481	The prepared hydrogels were investigated by FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis.					
36577473	2	35	theme	nitride	310:316	arg1	nanosheets					318:327	boron nitride nanosheets	304:327	boron nitride nanosheets aiming to enhancement in the adsorption rate	304:372	The prepared hydrogel was then modified using boron nitride nanosheets aiming to enhancement in the adsorption rate.					
36577473	4	36	contain	has	534:536	arg2	nature					558:563	a cytocompatibility nature	538:563	a cytocompatibility nature towards healthy normal human cell line (FR2 cells)	538:614	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	4	36	contain	has	534:536	arg1	sample					527:532	prepared sample	518:532	prepared sample	518:532	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	1	37	theme	various	233:239	arg1	environments					244:255	various pH environments	233:255	various pH environments	233:255	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36577473	1	38	theme	pH	241:242	arg1	environments					244:255	various pH environments	233:255	various pH environments	233:255	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36577473	3	39	theme	swelling	446:453	arg1	analysis					474:481	FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis	419:481	FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis	419:481	The prepared hydrogels were investigated by FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis.					
36577473	3	40	theme	viability	464:472	arg1	analysis					474:481	FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis	419:481	FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis	419:481	The prepared hydrogels were investigated by FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis.					
36577473	3	41	theme	TGA/DSC	437:443	arg1	analysis					474:481	FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis	419:481	FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis	419:481	The prepared hydrogels were investigated by FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis.					
36577473	4	42	theme	Cytotoxicity	484:495	arg1	study					497:501	Cytotoxicity study	484:501	Cytotoxicity study	484:501	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	2	43	theme	prepared	262:269	arg1	hydrogel					271:278	The prepared hydrogel	258:278	The prepared hydrogel	258:278	The prepared hydrogel was then modified using boron nitride nanosheets aiming to enhancement in the adsorption rate.					
36577473	5	44	theme	hydrogels	684:692	arg1	properties					666:675	the drug release properties	649:675	the drug release properties of the hydrogels	649:692	By changing the pH environment, the drug release properties of the hydrogels can be controlled.					
36577473	3	45	theme	FESEM	430:434	arg1	analysis					474:481	FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis	419:481	FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis	419:481	The prepared hydrogels were investigated by FTIR, XRD, FESEM, TGA/DSC, swelling and cell viability analysis.					
36577473	4	46	theme	normal	581:586	arg1	line					599:602	healthy normal human cell line	573:602	healthy normal human cell line (FR2 cells)	573:614	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	4	46	theme	normal	581:586	arg1	cells					609:613	FR2 cells	605:613	FR2 cells	605:613	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	7	47	from	pH = 7.4	932:939	arg1	%					904:904	78.6 %	899:904	78.6 % at pH = 2.2	899:916	Whereas drug release rate for NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite was 78.6 % at pH = 2.2 and 97.3 % at pH = 7.4 within 4320 min.					
36577473	7	47	from	pH = 7.4	932:939	arg1	%					927:927	97.3 %	922:927	97.3 % at pH = 7.4 within 4320 min	922:955	Whereas drug release rate for NaCMC-cl-DMAA/AAc-BNNSs hydrogel composite was 78.6 % at pH = 2.2 and 97.3 % at pH = 7.4 within 4320 min.					
36577473	4	48	theme	healthy	573:579	arg1	line					599:602	healthy normal human cell line	573:602	healthy normal human cell line (FR2 cells)	573:614	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	4	48	theme	healthy	573:579	arg1	cells					609:613	FR2 cells	605:613	FR2 cells	605:613	Cytotoxicity study indicated that prepared sample has a cytocompatibility nature towards healthy normal human cell line (FR2 cells).					
36577473	6	49	theme	NaCMC-cl-DMAA/AAc	748:764	arg1	hydrogel					766:773	NaCMC-cl-DMAA/AAc hydrogel	748:773	NaCMC-cl-DMAA/AAc hydrogel	748:773	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel was 76.5 % at pH = 2.2 and 87.5 % at pH = 7.4.					
36577473	2	50	theme	adsorption	358:367	arg1	rate					369:372	the adsorption rate	354:372	the adsorption rate	354:372	The prepared hydrogel was then modified using boron nitride nanosheets aiming to enhancement in the adsorption rate.					
36577473	0	51	theme	gentamicin	82:91	arg1	delivery					70:77	colon-specific delivery	55:77	colon-specific delivery of gentamicin	55:91	Carboxymethyl cellulose based sustainable hydrogel for colon-specific delivery of gentamicin.					
36577473	1	52	theme	NaCMC-cl-DMAA/AAc	154:170	arg1	hydrogel					172:179	NaCMC-cl-DMAA/AAc hydrogel	154:179	NaCMC-cl-DMAA/AAc hydrogel	154:179	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36577473	6	53	theme	release	736:742	arg1	%					784:784	76.5 %	779:784	76.5 % at pH = 2.2	779:796	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel was 76.5 % at pH = 2.2 and 87.5 % at pH = 7.4.					
36577473	6	53	theme	release	736:742	arg1	rate					728:731	The cumulative rate	713:731	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel	713:773	The cumulative rate of release for NaCMC-cl-DMAA/AAc hydrogel was 76.5 % at pH = 2.2 and 87.5 % at pH = 7.4.					
36577473	1	54	theme	hydrogel	172:179	arg1	improvement					139:149	improvement	139:149	improvement of NaCMC-cl-DMAA/AAc hydrogel	139:179	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36577473	1	54	theme	hydrogel	172:179	arg1	synthesis					128:136	synthesis	128:136	synthesis	128:136	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36577473	1	54	theme	hydrogel	172:179	arg1	release					204:210	in-situ controlled release	185:210	in-situ controlled release of gentamicin	185:224	The current research includes the synthesis, improvement of NaCMC-cl-DMAA/AAc hydrogel and in-situ controlled release of gentamicin within various pH environments.					
36347376	0	0	theme	compounds	94:102	arg1	control					71:77	release control	63:77	release control of hydrophilic compounds	63:102	Fabrication of soy protein isolate/κ-carrageenan hydrogels for release control of hydrophilic compounds: Flax lignans.					
36347376	7	1	theme	SPI	1115:1117	arg1	hydrogels					1119:1127	pure SPI hydrogels	1110:1127	pure SPI hydrogels	1110:1127	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	6	2	from	diffusion	956:964	arg1	SGF					935:937	SGF	935:937	SGF	935:937	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	6	2	from	diffusion	956:964	arg1	SIF					998:1000	SIF	998:1000	SIF	998:1000	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	6	2	from	diffusion	956:964	arg1	fluids					990:995	simulated intestinal fluids	969:995	simulated intestinal fluids (SIF)	969:1001	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	6	2	from	diffusion	956:964	arg1	fluids					927:932	simulated gastric fluids	909:932	simulated gastric fluids (SGF)	909:938	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	5	3	theme	Scanning	706:713	arg1	DSC					728:730	DSC	728:730	DSC	728:730	Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) curves suggested lignan might interact with SPI and KC by hydrogen bonding or hydrophobic effects.					
36347376	5	3	theme	Scanning	706:713	arg1	Calorimetry					715:725	Differential Scanning Calorimetry	693:725	Differential Scanning Calorimetry (DSC)	693:731	Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) curves suggested lignan might interact with SPI and KC by hydrogen bonding or hydrophobic effects.					
36347376	9	4	theme	water-soluble	1439:1451	arg1	compounds					1463:1471	water-soluble bioactive compounds	1439:1471	water-soluble bioactive compounds in food and pharmaceutical industries	1439:1509	These findings shield a light on SPI-KC hydrogels as carriers for water-soluble bioactive compounds in food and pharmaceutical industries.					
36347376	0	5	theme	hydrophilic	82:92	arg1	compounds					94:102	hydrophilic compounds	82:102	hydrophilic compounds	82:102	Fabrication of soy protein isolate/κ-carrageenan hydrogels for release control of hydrophilic compounds: Flax lignans.					
36347376	7	6	theme	flax	1049:1052	arg1	lignan					1054:1059	flax lignan	1049:1059	flax lignan in complex gels (46.00 %)	1049:1085	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	7	7	theme	cumulative	1022:1031	arg1	rate					1041:1044	The cumulative release rate	1018:1044	The cumulative release rate of flax lignan in complex gels (46.00 %)	1018:1085	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	7	7	theme	cumulative	1022:1031	arg1	lower					1091:1095	lower	1091:1095	lower	1091:1095	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	1	8	theme	Soybean	161:167	arg1	protein					169:175	Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels	161:221	protein	169:175	A suitable carrier for flax lignans using Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels was developed.					
36347376	1	8	theme	Soybean	161:167	arg1	SPI					187:189	SPI	187:189	SPI	187:189	A suitable carrier for flax lignans using Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels was developed.					
36347376	7	9	theme	lignan	1054:1059	arg1	rate					1041:1044	The cumulative release rate	1018:1044	The cumulative release rate of flax lignan in complex gels (46.00 %)	1018:1085	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	7	9	theme	lignan	1054:1059	arg1	lower					1091:1095	lower	1091:1095	lower	1091:1095	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	0	10	theme	Flax	105:108	arg1	lignans					110:116	Flax lignans	105:116	Flax lignans	105:116	Fabrication of soy protein isolate/κ-carrageenan hydrogels for release control of hydrophilic compounds: Flax lignans.					
36347376	6	11	theme	lignans	852:858	arg1	release					836:842	The release	832:842	The release of flax lignans in hydrogels	832:871	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	3	12	theme	rheological	483:493	arg1	test					504:507	rheological behavior test	483:507	rheological behavior test	483:507	A solid-like gel network and viscoelasticity of composite hydrogels were confirmed by rheological behavior test.					
36347376	7	13	dep	lower	1091:1095	arg1	%					1136:1136	77.43 %	1130:1136	77.43 %	1130:1136	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	5	14	dep	Transform	650:658	arg1	Infrared					660:667	Infrared	660:667	Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) curves	650:738	Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) curves suggested lignan might interact with SPI and KC by hydrogen bonding or hydrophobic effects.					
36347376	6	15	theme	flax	847:850	arg1	lignans					852:858	flax lignans	847:858	flax lignans	847:858	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	0	16	dep	lignans	110:116	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of soy protein isolate/κ-carrageenan hydrogels for release control of hydrophilic compounds	0:102	Fabrication of soy protein isolate/κ-carrageenan hydrogels for release control of hydrophilic compounds: Flax lignans.					
36347376	2	17	theme	concentration	256:268	arg1	effects					242:248	The effects	238:248	The effects of KC concentration on the stability of hydrogels	238:298	The effects of KC concentration on the stability of hydrogels were investigated, as well as water holding capacity (WHC), syneresis and morphological changes.					
36347376	3	18	theme	composite	445:453	arg1	hydrogels					455:463	composite hydrogels	445:463	composite hydrogels	445:463	A solid-like gel network and viscoelasticity of composite hydrogels were confirmed by rheological behavior test.					
36347376	5	19	theme	hydrogen	791:798	arg1	bonding					800:806	hydrogen bonding	791:806	hydrogen bonding	791:806	Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) curves suggested lignan might interact with SPI and KC by hydrogen bonding or hydrophobic effects.					
36347376	7	20	theme	digestion	1153:1161	arg1	end					1146:1148	the end	1142:1148	the end of digestion	1142:1161	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	7	21	theme	release	1033:1039	arg1	rate					1041:1044	The cumulative release rate	1018:1044	The cumulative release rate of flax lignan in complex gels (46.00 %)	1018:1085	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	7	21	theme	release	1033:1039	arg1	lower					1091:1095	lower	1091:1095	lower	1091:1095	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	9	22	from	compounds	1463:1471	arg1	industries					1500:1509	food and pharmaceutical industries	1476:1509	food and pharmaceutical industries	1476:1509	These findings shield a light on SPI-KC hydrogels as carriers for water-soluble bioactive compounds in food and pharmaceutical industries.					
36347376	2	23	theme	KC	253:254	arg1	concentration					256:268	KC concentration	253:268	KC concentration	253:268	The effects of KC concentration on the stability of hydrogels were investigated, as well as water holding capacity (WHC), syneresis and morphological changes.					
36347376	4	24	theme	dense	557:561	arg1	structure					575:583	a dense and uniform structure	555:583	a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %)	555:639	Scanning electron microscopy (SEM) displayed a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %).					
36347376	1	25	theme	isolated	177:184	arg1	protein					169:175	Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels	161:221	protein	169:175	A suitable carrier for flax lignans using Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels was developed.					
36347376	1	25	theme	isolated	177:184	arg1	SPI					187:189	SPI	187:189	SPI	187:189	A suitable carrier for flax lignans using Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels was developed.					
36347376	5	26	dep	Spectroscopy	669:680	arg1	curves					733:738	curves	733:738	curves	733:738	Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) curves suggested lignan might interact with SPI and KC by hydrogen bonding or hydrophobic effects.					
36347376	2	27	theme	hydrogels	290:298	arg1	stability					277:285	the stability	273:285	the stability of hydrogels	273:298	The effects of KC concentration on the stability of hydrogels were investigated, as well as water holding capacity (WHC), syneresis and morphological changes.					
36347376	4	28	theme	KC	616:617	arg1	concentration					619:631	the optimum KC concentration	604:631	the optimum KC concentration (0.6 %)	604:639	Scanning electron microscopy (SEM) displayed a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %).					
36347376	4	28	theme	KC	616:617	arg1	%					638:638	0.6 %	634:638	0.6 %	634:638	Scanning electron microscopy (SEM) displayed a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %).					
36347376	6	29	theme	simulated	969:977	arg1	SIF					998:1000	SIF	998:1000	SIF	998:1000	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	6	29	theme	simulated	969:977	arg1	fluids					990:995	simulated intestinal fluids	969:995	simulated intestinal fluids (SIF)	969:1001	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	6	30	from	release	836:842	arg1	hydrogels					863:871	hydrogels	863:871	hydrogels	863:871	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	5	31	dep	suggested	740:748	arg1	interact					763:770	interact	763:770	suggested lignan might interact with SPI and KC by hydrogen bonding or hydrophobic effects	740:829	Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) curves suggested lignan might interact with SPI and KC by hydrogen bonding or hydrophobic effects.					
36347376	5	32	theme	hydrophobic	811:821	arg1	effects					823:829	hydrophobic effects	811:829	hydrophobic effects	811:829	Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) curves suggested lignan might interact with SPI and KC by hydrogen bonding or hydrophobic effects.					
36347376	0	33	theme	protein	19:25	arg1	hydrogels					49:57	soy protein isolate/κ-carrageenan hydrogels	15:57	soy protein isolate/κ-carrageenan hydrogels	15:57	Fabrication of soy protein isolate/κ-carrageenan hydrogels for release control of hydrophilic compounds: Flax lignans.					
36347376	6	34	theme	gastric	919:925	arg1	SGF					935:937	SGF	935:937	SGF	935:937	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	6	34	theme	gastric	919:925	arg1	fluids					927:932	simulated gastric fluids	909:932	simulated gastric fluids (SGF)	909:938	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	7	35	from	lignan	1054:1059	arg1	gels					1072:1075	complex gels	1064:1075	complex gels (46.00 %)	1064:1085	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	7	35	from	lignan	1054:1059	arg1	%					1084:1084	46.00 %	1078:1084	46.00 %	1078:1084	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	2	36	theme	morphological	374:386	arg1	changes					388:394	morphological changes	374:394	morphological changes	374:394	The effects of KC concentration on the stability of hydrogels were investigated, as well as water holding capacity (WHC), syneresis and morphological changes.					
36347376	0	37	theme	soy	15:17	arg1	hydrogels					49:57	soy protein isolate/κ-carrageenan hydrogels	15:57	soy protein isolate/κ-carrageenan hydrogels	15:57	Fabrication of soy protein isolate/κ-carrageenan hydrogels for release control of hydrophilic compounds: Flax lignans.					
36347376	6	38	theme	simulated	909:917	arg1	SGF					935:937	SGF	935:937	SGF	935:937	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	6	38	theme	simulated	909:917	arg1	fluids					927:932	simulated gastric fluids	909:932	simulated gastric fluids (SGF)	909:938	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	8	39	theme	gel	1317:1319	arg1	erosion					1328:1334	gel matrix erosion	1317:1334	gel matrix erosion	1317:1334	The results indicated that KC protected the protein by hindering the accession of digestive enzymes into the hydrogels, thus resulting in a reduction of gel matrix erosion and lignan release during digestion.					
36347376	8	40	theme	enzymes	1256:1262	arg1	accession					1233:1241	the accession	1229:1241	the accession of digestive enzymes into the hydrogels	1229:1281	The results indicated that KC protected the protein by hindering the accession of digestive enzymes into the hydrogels, thus resulting in a reduction of gel matrix erosion and lignan release during digestion.					
36347376	2	41	from	effects	242:248	arg1	stability					277:285	the stability	273:285	the stability of hydrogels	273:298	The effects of KC concentration on the stability of hydrogels were investigated, as well as water holding capacity (WHC), syneresis and morphological changes.					
36347376	1	42	theme	flax	142:145	arg1	lignans					147:153	flax lignans	142:153	flax lignans using Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels	142:221	A suitable carrier for flax lignans using Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels was developed.					
36347376	0	43	theme	hydrogels	49:57	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of soy protein isolate/κ-carrageenan hydrogels for release control of hydrophilic compounds	0:102	Fabrication of soy protein isolate/κ-carrageenan hydrogels for release control of hydrophilic compounds: Flax lignans.					
36347376	7	44	from	rate	1041:1044	arg1	gels					1072:1075	complex gels	1064:1075	complex gels (46.00 %)	1064:1085	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	7	44	from	rate	1041:1044	arg1	%					1084:1084	46.00 %	1078:1084	46.00 %	1078:1084	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	3	45	theme	gel	410:412	arg1	network					414:420	A solid-like gel network	397:420	A solid-like gel network	397:420	A solid-like gel network and viscoelasticity of composite hydrogels were confirmed by rheological behavior test.					
36347376	4	46	with	hydrogels	589:597	arg1	concentration					619:631	the optimum KC concentration	604:631	the optimum KC concentration (0.6 %)	604:639	Scanning electron microscopy (SEM) displayed a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %).					
36347376	4	46	with	hydrogels	589:597	arg1	%					638:638	0.6 %	634:638	0.6 %	634:638	Scanning electron microscopy (SEM) displayed a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %).					
36347376	1	47	theme	κ-carrageenan	194:206	arg1	hydrogels					213:221	Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels	161:221	hydrogels	213:221	A suitable carrier for flax lignans using Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels was developed.					
36347376	1	47	theme	κ-carrageenan	194:206	arg1	KC					209:210	KC	209:210	KC	209:210	A suitable carrier for flax lignans using Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels was developed.					
36347376	0	48	theme	isolate/κ-carrageenan	27:47	arg1	hydrogels					49:57	soy protein isolate/κ-carrageenan hydrogels	15:57	soy protein isolate/κ-carrageenan hydrogels	15:57	Fabrication of soy protein isolate/κ-carrageenan hydrogels for release control of hydrophilic compounds: Flax lignans.					
36347376	8	49	theme	erosion	1328:1334	arg1	reduction					1304:1312	a reduction	1302:1312	a reduction of gel matrix erosion and lignan release	1302:1353	The results indicated that KC protected the protein by hindering the accession of digestive enzymes into the hydrogels, thus resulting in a reduction of gel matrix erosion and lignan release during digestion.					
36347376	9	50	theme	food	1476:1479	arg1	industries					1500:1509	food and pharmaceutical industries	1476:1509	food and pharmaceutical industries	1476:1509	These findings shield a light on SPI-KC hydrogels as carriers for water-soluble bioactive compounds in food and pharmaceutical industries.					
36347376	4	51	theme	optimum	608:614	arg1	concentration					619:631	the optimum KC concentration	604:631	the optimum KC concentration (0.6 %)	604:639	Scanning electron microscopy (SEM) displayed a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %).					
36347376	4	51	theme	optimum	608:614	arg1	%					638:638	0.6 %	634:638	0.6 %	634:638	Scanning electron microscopy (SEM) displayed a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %).					
36347376	0	52	theme	release	63:69	arg1	control					71:77	release control	63:77	release control of hydrophilic compounds	63:102	Fabrication of soy protein isolate/κ-carrageenan hydrogels for release control of hydrophilic compounds: Flax lignans.					
36347376	5	53	dep	Fourier	642:648	arg1	Transform					650:658	Transform	650:658	Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) curves	650:738	Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) curves suggested lignan might interact with SPI and KC by hydrogen bonding or hydrophobic effects.					
36347376	8	54	theme	digestive	1246:1254	arg1	enzymes					1256:1262	digestive enzymes	1246:1262	digestive enzymes	1246:1262	The results indicated that KC protected the protein by hindering the accession of digestive enzymes into the hydrogels, thus resulting in a reduction of gel matrix erosion and lignan release during digestion.					
36347376	8	55	theme	matrix	1321:1326	arg1	erosion					1328:1334	gel matrix erosion	1317:1334	gel matrix erosion	1317:1334	The results indicated that KC protected the protein by hindering the accession of digestive enzymes into the hydrogels, thus resulting in a reduction of gel matrix erosion and lignan release during digestion.					
36347376	8	56	theme	release	1347:1353	arg1	reduction					1304:1312	a reduction	1302:1312	a reduction of gel matrix erosion and lignan release	1302:1353	The results indicated that KC protected the protein by hindering the accession of digestive enzymes into the hydrogels, thus resulting in a reduction of gel matrix erosion and lignan release during digestion.					
36347376	3	57	theme	hydrogels	455:463	arg1	network					414:420	A solid-like gel network	397:420	A solid-like gel network	397:420	A solid-like gel network and viscoelasticity of composite hydrogels were confirmed by rheological behavior test.					
36347376	3	57	theme	hydrogels	455:463	arg1	viscoelasticity					426:440	viscoelasticity	426:440	viscoelasticity of composite hydrogels	426:463	A solid-like gel network and viscoelasticity of composite hydrogels were confirmed by rheological behavior test.					
36347376	4	58	theme	electron	519:526	arg1	SEM					540:542	SEM	540:542	SEM	540:542	Scanning electron microscopy (SEM) displayed a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %).					
36347376	4	58	theme	electron	519:526	arg1	microscopy					528:537	Scanning electron microscopy	510:537	Scanning electron microscopy (SEM)	510:543	Scanning electron microscopy (SEM) displayed a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %).					
36347376	8	59	theme	lignan	1340:1345	arg1	release					1347:1353	lignan release	1340:1353	lignan release	1340:1353	The results indicated that KC protected the protein by hindering the accession of digestive enzymes into the hydrogels, thus resulting in a reduction of gel matrix erosion and lignan release during digestion.					
36347376	9	60	theme	pharmaceutical	1485:1498	arg1	industries					1500:1509	food and pharmaceutical industries	1476:1509	food and pharmaceutical industries	1476:1509	These findings shield a light on SPI-KC hydrogels as carriers for water-soluble bioactive compounds in food and pharmaceutical industries.					
36347376	7	61	theme	pure	1110:1113	arg1	hydrogels					1119:1127	pure SPI hydrogels	1110:1127	pure SPI hydrogels	1110:1127	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	1	62	theme	suitable	121:128	arg1	carrier					130:136	A suitable carrier	119:136	A suitable carrier for flax lignans using Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels	119:221	A suitable carrier for flax lignans using Soybean protein isolated (SPI) - κ-carrageenan (KC) hydrogels was developed.					
36347376	4	63	theme	Scanning	510:517	arg1	SEM					540:542	SEM	540:542	SEM	540:542	Scanning electron microscopy (SEM) displayed a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %).					
36347376	4	63	theme	Scanning	510:517	arg1	microscopy					528:537	Scanning electron microscopy	510:537	Scanning electron microscopy (SEM)	510:543	Scanning electron microscopy (SEM) displayed a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %).					
36347376	6	64	from	diffusion	896:904	arg1	SGF					935:937	SGF	935:937	SGF	935:937	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	6	64	from	diffusion	896:904	arg1	SIF					998:1000	SIF	998:1000	SIF	998:1000	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	6	64	from	diffusion	896:904	arg1	fluids					990:995	simulated intestinal fluids	969:995	simulated intestinal fluids (SIF)	969:1001	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	6	64	from	diffusion	896:904	arg1	fluids					927:932	simulated gastric fluids	909:932	simulated gastric fluids (SGF)	909:938	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	7	65	from	gels	1072:1075	arg1	rate					1041:1044	The cumulative release rate	1018:1044	The cumulative release rate of flax lignan in complex gels (46.00 %)	1018:1085	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	7	65	from	gels	1072:1075	arg1	lower					1091:1095	lower	1091:1095	lower	1091:1095	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	9	66	theme	bioactive	1453:1461	arg1	compounds					1463:1471	water-soluble bioactive compounds	1439:1471	water-soluble bioactive compounds in food and pharmaceutical industries	1439:1509	These findings shield a light on SPI-KC hydrogels as carriers for water-soluble bioactive compounds in food and pharmaceutical industries.					
36347376	5	67	theme	Differential	693:704	arg1	DSC					728:730	DSC	728:730	DSC	728:730	Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) curves suggested lignan might interact with SPI and KC by hydrogen bonding or hydrophobic effects.					
36347376	5	67	theme	Differential	693:704	arg1	Calorimetry					715:725	Differential Scanning Calorimetry	693:725	Differential Scanning Calorimetry (DSC)	693:731	Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) curves suggested lignan might interact with SPI and KC by hydrogen bonding or hydrophobic effects.					
36347376	9	68	theme	SPI-KC	1406:1411	arg1	hydrogels					1413:1421	SPI-KC hydrogels	1406:1421	SPI-KC hydrogels as carriers for water-soluble bioactive compounds in food and pharmaceutical industries	1406:1509	These findings shield a light on SPI-KC hydrogels as carriers for water-soluble bioactive compounds in food and pharmaceutical industries.					
36347376	2	69	theme	water	330:334	arg1	WHC					354:356	WHC	354:356	WHC	354:356	The effects of KC concentration on the stability of hydrogels were investigated, as well as water holding capacity (WHC), syneresis and morphological changes.					
36347376	2	69	theme	water	330:334	arg1	capacity					344:351	water holding capacity	330:351	water holding capacity (WHC)	330:357	The effects of KC concentration on the stability of hydrogels were investigated, as well as water holding capacity (WHC), syneresis and morphological changes.					
36347376	4	70	theme	uniform	567:573	arg1	structure					575:583	a dense and uniform structure	555:583	a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %)	555:639	Scanning electron microscopy (SEM) displayed a dense and uniform structure for hydrogels with the optimum KC concentration (0.6 %).					
36347376	6	71	theme	intestinal	979:988	arg1	SIF					998:1000	SIF	998:1000	SIF	998:1000	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	6	71	theme	intestinal	979:988	arg1	fluids					990:995	simulated intestinal fluids	969:995	simulated intestinal fluids (SIF)	969:1001	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	2	72	theme	holding	336:342	arg1	WHC					354:356	WHC	354:356	WHC	354:356	The effects of KC concentration on the stability of hydrogels were investigated, as well as water holding capacity (WHC), syneresis and morphological changes.					
36347376	2	72	theme	holding	336:342	arg1	capacity					344:351	water holding capacity	330:351	water holding capacity (WHC)	330:357	The effects of KC concentration on the stability of hydrogels were investigated, as well as water holding capacity (WHC), syneresis and morphological changes.					
36347376	3	73	theme	solid-like	399:408	arg1	network					414:420	A solid-like gel network	397:420	A solid-like gel network	397:420	A solid-like gel network and viscoelasticity of composite hydrogels were confirmed by rheological behavior test.					
36347376	7	74	theme	complex	1064:1070	arg1	gels					1072:1075	complex gels	1064:1075	complex gels (46.00 %)	1064:1085	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	7	74	theme	complex	1064:1070	arg1	%					1084:1084	46.00 %	1078:1084	46.00 %	1078:1084	The cumulative release rate of flax lignan in complex gels (46.00 %) was lower than that of pure SPI hydrogels (77.43 %) at the end of digestion.					
36347376	6	75	theme	non-Fickian	944:954	arg1	diffusion					956:964	non-Fickian diffusion	944:964	non-Fickian diffusion in simulated intestinal fluids (SIF), respectively	944:1015	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
36347376	3	76	theme	behavior	495:502	arg1	test					504:507	rheological behavior test	483:507	rheological behavior test	483:507	A solid-like gel network and viscoelasticity of composite hydrogels were confirmed by rheological behavior test.					
36347376	6	77	theme	Fick	891:894	arg1	diffusion					896:904	Fick diffusion	891:904	Fick diffusion in simulated gastric fluids (SGF)	891:938	The release of flax lignans in hydrogels was followed with Fick diffusion in simulated gastric fluids (SGF) and non-Fickian diffusion in simulated intestinal fluids (SIF), respectively.					
37175077	8	0	theme	biofilm	1388:1394	arg1	development					1362:1372	the development	1358:1372	the development of hyphae and biofilm in C. albicans DAY185	1358:1416	Therefore, QRC:β-CD-K MOFs had no effect on the growth of planktonic cells while inhibiting the development of hyphae and biofilm in C. albicans DAY185.					
37175077	4	1	theme	QRC	826:828	arg1	interaction					811:821	the interaction	807:821	the interaction of QRC with the β-CD-K MOFs	807:849	The shifting in the chemical shift values of QRC in the MOFs may be the reason for the interaction of QRC with the β-CD-K MOFs.					
37175077	8	2	theme	planktonic	1324:1333	arg1	cells					1335:1339	planktonic cells	1324:1339	planktonic cells	1324:1339	Therefore, QRC:β-CD-K MOFs had no effect on the growth of planktonic cells while inhibiting the development of hyphae and biofilm in C. albicans DAY185.					
37175077	7	3	theme	aggregation	1153:1163	arg1	consistent					1187:1196	consistent	1187:1196	consistent	1187:1196	The results of the cell aggregation and hyphal growth are consistent with the antibiofilm activity that is found in the treatment group.					
37175077	7	3	theme	aggregation	1153:1163	arg1	results					1133:1139	The results	1129:1139	The results of the cell aggregation and hyphal growth	1129:1181	The results of the cell aggregation and hyphal growth are consistent with the antibiofilm activity that is found in the treatment group.					
37175077	0	4	theme	Antifungal	103:112	arg1	Agent					114:118	Antifungal Agent	103:118	Antifungal Agent	103:118	Supramolecular β-Cyclodextrin-Quercetin Based Metal-Organic Frameworks as an Efficient Antibiofilm and Antifungal Agent.					
37175077	8	5	contain	had	1293:1295	arg2	effect					1300:1305	no effect	1297:1305	no effect	1297:1305	Therefore, QRC:β-CD-K MOFs had no effect on the growth of planktonic cells while inhibiting the development of hyphae and biofilm in C. albicans DAY185.					
37175077	8	5	contain	had	1293:1295	arg1	MOFs					1288:1291	β-CD-K MOFs	1281:1291	β-CD-K MOFs	1281:1291	Therefore, QRC:β-CD-K MOFs had no effect on the growth of planktonic cells while inhibiting the development of hyphae and biofilm in C. albicans DAY185.					
37175077	2	6	from	application	422:432	arg1	framework					472:480	a cyclodextrin-based metal-organic framework	437:480	a cyclodextrin-based metal-organic framework via a solvent diffusion approach	437:513	The quercetin (QRC) loading and biofilm application on a cyclodextrin-based metal-organic framework via a solvent diffusion approach is successfully accomplished in the current study.					
37175077	4	7	theme	chemical	744:751	arg1	values					759:764	the chemical shift values	740:764	the chemical shift values of QRC	740:771	The shifting in the chemical shift values of QRC in the MOFs may be the reason for the interaction of QRC with the β-CD-K MOFs.					
37175077	1	8	theme	new	267:269	arg1	frameworks					222:231	metal-organic frameworks	208:231	metal-organic frameworks (MOFs)	208:238	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	1	8	theme	new	267:269	arg1	type					271:274	a new type	265:274	a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure	265:379	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	7	9	dep	aggregation	1153:1163	arg1	the					1144:1146	the	1144:1146	the	1144:1146	The results of the cell aggregation and hyphal growth are consistent with the antibiofilm activity that is found in the treatment group.					
37175077	3	10	theme	β-CD-K	711:716	arg1	QRC					600:602	The antibacterial plant flavonoid QRC	566:602	The antibacterial plant flavonoid QRC	566:602	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	3	10	theme	β-CD-K	711:716	arg1	MOFs					718:721	QRC:β-CD-K MOFs	707:721	QRC:β-CD-K MOFs	707:721	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	9	11	theme	new	1438:1440	arg1	opportunities					1442:1454	new opportunities	1438:1454	new opportunities for supramolecular β-CD-based MOF development for use in biological research and pharmaceutical production	1438:1561	This study creates new opportunities for supramolecular β-CD-based MOF development for use in biological research and pharmaceutical production.					
37175077	5	12	theme	relative	877:884	arg1	contents					886:893	relative contents	877:893	relative contents	877:893	The binding energies and relative contents of MOFs are considerably changed after the formation of QRC:β-CD-K MOFs, suggesting that the interactions took place during the loading of QRC.					
37175077	5	13	theme	binding	856:862	arg1	energies					864:871	The binding energies	852:871	The binding energies	852:871	The binding energies and relative contents of MOFs are considerably changed after the formation of QRC:β-CD-K MOFs, suggesting that the interactions took place during the loading of QRC.					
37175077	6	14	from	reduction	1090:1098	arg1	formation					1107:1115	the formation	1103:1115	the formation of biofilm	1103:1126	Confocal laser scanning microscopy (CLSM) showed a reduction in the formation of biofilm.					
37175077	2	15	theme	quercetin	386:394	arg1	loading					402:408	The quercetin (QRC) loading	382:408	The quercetin (QRC) loading	382:408	The quercetin (QRC) loading and biofilm application on a cyclodextrin-based metal-organic framework via a solvent diffusion approach is successfully accomplished in the current study.					
37175077	8	16	from	development	1362:1372	arg1	albicans					1402:1409	C. albicans DAY185	1399:1416	C. albicans DAY185	1399:1416	Therefore, QRC:β-CD-K MOFs had no effect on the growth of planktonic cells while inhibiting the development of hyphae and biofilm in C. albicans DAY185.					
37175077	3	17	theme	QRC	707:709	arg1	QRC					600:602	The antibacterial plant flavonoid QRC	566:602	The antibacterial plant flavonoid QRC	566:602	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	3	17	theme	QRC	707:709	arg1	MOFs					718:721	QRC:β-CD-K MOFs	707:721	QRC:β-CD-K MOFs	707:721	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	7	18	with	consistent	1187:1196	arg1	activity					1219:1226	the antibiofilm activity	1203:1226	the antibiofilm activity that is found in the treatment group	1203:1263	The results of the cell aggregation and hyphal growth are consistent with the antibiofilm activity that is found in the treatment group.					
37175077	4	19	theme	shift	753:757	arg1	values					759:764	the chemical shift values	740:764	the chemical shift values of QRC	740:771	The shifting in the chemical shift values of QRC in the MOFs may be the reason for the interaction of QRC with the β-CD-K MOFs.					
37175077	7	20	theme	treatment	1249:1257	arg1	group					1259:1263	the treatment group	1245:1263	the treatment group	1245:1263	The results of the cell aggregation and hyphal growth are consistent with the antibiofilm activity that is found in the treatment group.					
37175077	1	21	theme	network	363:369	arg1	structure					371:379	a network structure	361:379	a network structure	361:379	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	7	22	theme	antibiofilm	1207:1217	arg1	activity					1219:1226	the antibiofilm activity	1203:1226	the antibiofilm activity that is found in the treatment group	1203:1263	The results of the cell aggregation and hyphal growth are consistent with the antibiofilm activity that is found in the treatment group.					
37175077	4	23	from	shifting	728:735	arg1	MOFs					780:783	the MOFs	776:783	the MOFs	776:783	The shifting in the chemical shift values of QRC in the MOFs may be the reason for the interaction of QRC with the β-CD-K MOFs.					
37175077	4	23	from	shifting	728:735	arg1	values					759:764	the chemical shift values	740:764	the chemical shift values of QRC	740:771	The shifting in the chemical shift values of QRC in the MOFs may be the reason for the interaction of QRC with the β-CD-K MOFs.					
37175077	3	24	theme	antibacterial	570:582	arg1	QRC					600:602	The antibacterial plant flavonoid QRC	566:602	The antibacterial plant flavonoid QRC	566:602	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	3	24	theme	antibacterial	570:582	arg1	MOFs					718:721	QRC:β-CD-K MOFs	707:721	QRC:β-CD-K MOFs	707:721	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	5	25	theme	QRC	1034:1036	arg1	loading					1023:1029	the loading	1019:1029	the loading of QRC	1019:1036	The binding energies and relative contents of MOFs are considerably changed after the formation of QRC:β-CD-K MOFs, suggesting that the interactions took place during the loading of QRC.					
37175077	1	26	theme	metal-organic	208:220	arg1	MOFs					234:237	MOFs	234:237	MOFs	234:237	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	1	26	theme	metal-organic	208:220	arg1	frameworks					222:231	metal-organic frameworks	208:231	metal-organic frameworks (MOFs)	208:238	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	1	26	theme	metal-organic	208:220	arg1	type					271:274	a new type	265:274	a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure	265:379	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	0	27	theme	Supramolecular	0:13	arg1	β-Cyclodextrin-Quercetin					15:38	Supramolecular β-Cyclodextrin-Quercetin	0:38	Supramolecular β-Cyclodextrin-Quercetin	0:38	Supramolecular β-Cyclodextrin-Quercetin Based Metal-Organic Frameworks as an Efficient Antibiofilm and Antifungal Agent.					
37175077	6	28	theme	biofilm	1120:1126	arg1	formation					1107:1115	the formation	1103:1115	the formation of biofilm	1103:1126	Confocal laser scanning microscopy (CLSM) showed a reduction in the formation of biofilm.					
37175077	8	29	theme	hyphae	1377:1382	arg1	development					1362:1372	the development	1358:1372	the development of hyphae and biofilm in C. albicans DAY185	1358:1416	Therefore, QRC:β-CD-K MOFs had no effect on the growth of planktonic cells while inhibiting the development of hyphae and biofilm in C. albicans DAY185.					
37175077	3	30	theme	plant	584:588	arg1	QRC					600:602	The antibacterial plant flavonoid QRC	566:602	The antibacterial plant flavonoid QRC	566:602	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	3	30	theme	plant	584:588	arg1	MOFs					718:721	QRC:β-CD-K MOFs	707:721	QRC:β-CD-K MOFs	707:721	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	8	31	theme	cells	1335:1339	arg1	growth					1314:1319	the growth	1310:1319	the growth of planktonic cells	1310:1339	Therefore, QRC:β-CD-K MOFs had no effect on the growth of planktonic cells while inhibiting the development of hyphae and biofilm in C. albicans DAY185.					
37175077	0	32	theme	Metal-Organic	46:58	arg1	Antibiofilm					87:97	an Efficient Antibiofilm	74:97	an Efficient Antibiofilm	74:97	Supramolecular β-Cyclodextrin-Quercetin Based Metal-Organic Frameworks as an Efficient Antibiofilm and Antifungal Agent.					
37175077	0	32	theme	Metal-Organic	46:58	arg1	Frameworks					60:69	Metal-Organic Frameworks	46:69	Metal-Organic Frameworks	46:69	Supramolecular β-Cyclodextrin-Quercetin Based Metal-Organic Frameworks as an Efficient Antibiofilm and Antifungal Agent.					
37175077	1	33	theme	porous	279:284	arg1	material					286:293	porous material	279:293	porous material	279:293	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	7	34	theme	hyphal	1169:1174	arg1	growth					1176:1181	hyphal growth	1169:1181	hyphal growth	1169:1181	The results of the cell aggregation and hyphal growth are consistent with the antibiofilm activity that is found in the treatment group.					
37175077	5	35	theme	MOFs	898:901	arg1	energies					864:871	The binding energies	852:871	The binding energies	852:871	The binding energies and relative contents of MOFs are considerably changed after the formation of QRC:β-CD-K MOFs, suggesting that the interactions took place during the loading of QRC.					
37175077	5	35	theme	MOFs	898:901	arg1	contents					886:893	relative contents	877:893	relative contents	877:893	The binding energies and relative contents of MOFs are considerably changed after the formation of QRC:β-CD-K MOFs, suggesting that the interactions took place during the loading of QRC.					
37175077	6	36	theme	laser	1048:1052	arg1	CLSM					1075:1078	CLSM	1075:1078	CLSM	1075:1078	Confocal laser scanning microscopy (CLSM) showed a reduction in the formation of biofilm.					
37175077	6	36	theme	laser	1048:1052	arg1	microscopy					1063:1072	Confocal laser scanning microscopy	1039:1072	Confocal laser scanning microscopy (CLSM)	1039:1079	Confocal laser scanning microscopy (CLSM) showed a reduction in the formation of biofilm.					
37175077	1	37	theme	material	286:293	arg1	frameworks					222:231	metal-organic frameworks	208:231	metal-organic frameworks (MOFs)	208:238	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	1	37	theme	material	286:293	arg1	type					271:274	a new type	265:274	a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure	265:379	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	2	38	theme	current	551:557	arg1	study					559:563	the current study	547:563	the current study	547:563	The quercetin (QRC) loading and biofilm application on a cyclodextrin-based metal-organic framework via a solvent diffusion approach is successfully accomplished in the current study.					
37175077	3	39	theme	composite	645:653	arg1	complexes					676:684	the composite containing inclusion complexes	641:684	the composite containing inclusion complexes (ICs)	641:690	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	3	39	theme	composite	645:653	arg1	ICs					687:689	ICs	687:689	ICs	687:689	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	8	40	theme	β-CD-K	1281:1286	arg1	MOFs					1288:1291	β-CD-K MOFs	1281:1291	β-CD-K MOFs	1281:1291	Therefore, QRC:β-CD-K MOFs had no effect on the growth of planktonic cells while inhibiting the development of hyphae and biofilm in C. albicans DAY185.					
37175077	6	41	theme	Confocal	1039:1046	arg1	CLSM					1075:1078	CLSM	1075:1078	CLSM	1075:1078	Confocal laser scanning microscopy (CLSM) showed a reduction in the formation of biofilm.					
37175077	6	41	theme	Confocal	1039:1046	arg1	microscopy					1063:1072	Confocal laser scanning microscopy	1039:1072	Confocal laser scanning microscopy (CLSM)	1039:1079	Confocal laser scanning microscopy (CLSM) showed a reduction in the formation of biofilm.					
37175077	9	42	theme	supramolecular	1460:1473	arg1	development					1490:1500	supramolecular β-CD-based MOF development	1460:1500	supramolecular β-CD-based MOF development	1460:1500	This study creates new opportunities for supramolecular β-CD-based MOF development for use in biological research and pharmaceutical production.					
37175077	3	43	theme	containing	655:664	arg1	complexes					676:684	the composite containing inclusion complexes	641:684	the composite containing inclusion complexes (ICs)	641:690	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	3	43	theme	containing	655:664	arg1	ICs					687:689	ICs	687:689	ICs	687:689	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	5	44	theme	β-CD-K	955:960	arg1	MOFs					962:965	QRC:β-CD-K MOFs	951:965	QRC:β-CD-K MOFs	951:965	The binding energies and relative contents of MOFs are considerably changed after the formation of QRC:β-CD-K MOFs, suggesting that the interactions took place during the loading of QRC.					
37175077	9	45	from	use	1506:1508	arg1	research					1524:1531	biological research	1513:1531	biological research	1513:1531	This study creates new opportunities for supramolecular β-CD-based MOF development for use in biological research and pharmaceutical production.					
37175077	9	45	from	use	1506:1508	arg1	production					1552:1561	pharmaceutical production	1537:1561	pharmaceutical production	1537:1561	This study creates new opportunities for supramolecular β-CD-based MOF development for use in biological research and pharmaceutical production.					
37175077	2	46	theme	QRC	397:399	arg1	loading					402:408	The quercetin (QRC) loading	382:408	The quercetin (QRC) loading	382:408	The quercetin (QRC) loading and biofilm application on a cyclodextrin-based metal-organic framework via a solvent diffusion approach is successfully accomplished in the current study.					
37175077	2	47	theme	metal-organic	458:470	arg1	framework					472:480	a cyclodextrin-based metal-organic framework	437:480	a cyclodextrin-based metal-organic framework via a solvent diffusion approach	437:513	The quercetin (QRC) loading and biofilm application on a cyclodextrin-based metal-organic framework via a solvent diffusion approach is successfully accomplished in the current study.					
37175077	2	48	theme	biofilm	414:420	arg1	application					422:432	biofilm application	414:432	biofilm application	414:432	The quercetin (QRC) loading and biofilm application on a cyclodextrin-based metal-organic framework via a solvent diffusion approach is successfully accomplished in the current study.					
37175077	9	49	theme	pharmaceutical	1537:1550	arg1	production					1552:1561	pharmaceutical production	1537:1561	pharmaceutical production	1537:1561	This study creates new opportunities for supramolecular β-CD-based MOF development for use in biological research and pharmaceutical production.					
37175077	2	50	theme	cyclodextrin-based	439:456	arg1	framework					472:480	a cyclodextrin-based metal-organic framework	437:480	a cyclodextrin-based metal-organic framework via a solvent diffusion approach	437:513	The quercetin (QRC) loading and biofilm application on a cyclodextrin-based metal-organic framework via a solvent diffusion approach is successfully accomplished in the current study.					
37175077	1	51	theme	organic	304:310	arg1	ligands					312:318	organic ligands	304:318	organic ligands	304:318	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	2	52	theme	solvent	488:494	arg1	approach					506:513	a solvent diffusion approach	486:513	a solvent diffusion approach	486:513	The quercetin (QRC) loading and biofilm application on a cyclodextrin-based metal-organic framework via a solvent diffusion approach is successfully accomplished in the current study.					
37175077	3	53	theme	flavonoid	590:598	arg1	QRC					600:602	The antibacterial plant flavonoid QRC	566:602	The antibacterial plant flavonoid QRC	566:602	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	3	53	theme	flavonoid	590:598	arg1	MOFs					718:721	QRC:β-CD-K MOFs	707:721	QRC:β-CD-K MOFs	707:721	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	1	54	theme	drugs	136:140	arg1	loading					125:131	The loading	121:131	The loading of drugs or medicinally active compounds	121:172	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	8	55	theme	C.	1399:1400	arg1	albicans					1402:1409	C. albicans DAY185	1399:1416	C. albicans DAY185	1399:1416	Therefore, QRC:β-CD-K MOFs had no effect on the growth of planktonic cells while inhibiting the development of hyphae and biofilm in C. albicans DAY185.					
37175077	4	56	theme	β-CD-K	839:844	arg1	MOFs					846:849	the β-CD-K MOFs	835:849	the β-CD-K MOFs	835:849	The shifting in the chemical shift values of QRC in the MOFs may be the reason for the interaction of QRC with the β-CD-K MOFs.					
37175077	9	57	theme	biological	1513:1522	arg1	research					1524:1531	biological research	1513:1531	biological research	1513:1531	This study creates new opportunities for supramolecular β-CD-based MOF development for use in biological research and pharmaceutical production.					
37175077	9	58	theme	β-CD-based	1475:1484	arg1	development					1490:1500	supramolecular β-CD-based MOF development	1460:1500	supramolecular β-CD-based MOF development	1460:1500	This study creates new opportunities for supramolecular β-CD-based MOF development for use in biological research and pharmaceutical production.					
37175077	4	59	theme	QRC	769:771	arg1	values					759:764	the chemical shift values	740:764	the chemical shift values of QRC	740:771	The shifting in the chemical shift values of QRC in the MOFs may be the reason for the interaction of QRC with the β-CD-K MOFs.					
37175077	5	60	theme	MOFs	962:965	arg1	formation					938:946	the formation	934:946	the formation of QRC:β-CD-K MOFs	934:965	The binding energies and relative contents of MOFs are considerably changed after the formation of QRC:β-CD-K MOFs, suggesting that the interactions took place during the loading of QRC.					
37175077	1	61	theme	medicinally	145:155	arg1	compounds					164:172	medicinally active compounds	145:172	medicinally active compounds	145:172	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	9	62	theme	MOF	1486:1488	arg1	development					1490:1500	supramolecular β-CD-based MOF development	1460:1500	supramolecular β-CD-based MOF development	1460:1500	This study creates new opportunities for supramolecular β-CD-based MOF development for use in biological research and pharmaceutical production.					
37175077	3	63	theme	inclusion	666:674	arg1	complexes					676:684	the composite containing inclusion complexes	641:684	the composite containing inclusion complexes (ICs)	641:690	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	3	63	theme	inclusion	666:674	arg1	ICs					687:689	ICs	687:689	ICs	687:689	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	1	64	theme	active	157:162	arg1	compounds					164:172	medicinally active compounds	145:172	medicinally active compounds	145:172	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	6	65	theme	scanning	1054:1061	arg1	CLSM					1075:1078	CLSM	1075:1078	CLSM	1075:1078	Confocal laser scanning microscopy (CLSM) showed a reduction in the formation of biofilm.					
37175077	6	65	theme	scanning	1054:1061	arg1	microscopy					1063:1072	Confocal laser scanning microscopy	1039:1072	Confocal laser scanning microscopy (CLSM)	1039:1079	Confocal laser scanning microscopy (CLSM) showed a reduction in the formation of biofilm.					
37175077	2	66	theme	diffusion	496:504	arg1	approach					506:513	a solvent diffusion approach	486:513	a solvent diffusion approach	486:513	The quercetin (QRC) loading and biofilm application on a cyclodextrin-based metal-organic framework via a solvent diffusion approach is successfully accomplished in the current study.					
37175077	7	67	located	found	1236:1240	arg1	group					1259:1263	the treatment group	1245:1263	the treatment group	1245:1263	The results of the cell aggregation and hyphal growth are consistent with the antibiofilm activity that is found in the treatment group.					
37175077	7	67	located	found	1236:1240	arg2	activity					1219:1226	the antibiofilm activity	1203:1226	the antibiofilm activity that is found in the treatment group	1203:1263	The results of the cell aggregation and hyphal growth are consistent with the antibiofilm activity that is found in the treatment group.					
37175077	5	68	theme	QRC	951:953	arg1	MOFs					962:965	QRC:β-CD-K MOFs	951:965	QRC:β-CD-K MOFs	951:965	The binding energies and relative contents of MOFs are considerably changed after the formation of QRC:β-CD-K MOFs, suggesting that the interactions took place during the loading of QRC.					
37175077	1	69	theme	compounds	164:172	arg1	loading					125:131	The loading	121:131	The loading of drugs or medicinally active compounds	121:172	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	7	70	theme	cell	1148:1151	arg1	aggregation					1153:1163	cell aggregation	1148:1163	cell aggregation	1148:1163	The results of the cell aggregation and hyphal growth are consistent with the antibiofilm activity that is found in the treatment group.					
37175077	0	71	theme	Efficient	77:85	arg1	Antibiofilm					87:97	an Efficient Antibiofilm	74:97	an Efficient Antibiofilm	74:97	Supramolecular β-Cyclodextrin-Quercetin Based Metal-Organic Frameworks as an Efficient Antibiofilm and Antifungal Agent.					
37175077	0	71	theme	Efficient	77:85	arg1	Frameworks					60:69	Metal-Organic Frameworks	46:69	Metal-Organic Frameworks	46:69	Supramolecular β-Cyclodextrin-Quercetin Based Metal-Organic Frameworks as an Efficient Antibiofilm and Antifungal Agent.					
37175077	1	72	theme	metal	324:328	arg1	ions					330:333	metal ions	324:333	metal ions	324:333	The loading of drugs or medicinally active compounds has recently been performed using metal-organic frameworks (MOFs), which are thought to be a new type of porous material in which organic ligands and metal ions can self-assemble to form a network structure.					
37175077	3	73	theme	β-CD-K	619:624	arg1	MOFs					626:629	β-CD-K MOFs	619:629	β-CD-K MOFs	619:629	The antibacterial plant flavonoid QRC is loaded onto β-CD-K MOFs to create the composite containing inclusion complexes (ICs) and denoted as QRC:β-CD-K MOFs.					
37175077	2	74	from	loading	402:408	arg1	framework					472:480	a cyclodextrin-based metal-organic framework	437:480	a cyclodextrin-based metal-organic framework via a solvent diffusion approach	437:513	The quercetin (QRC) loading and biofilm application on a cyclodextrin-based metal-organic framework via a solvent diffusion approach is successfully accomplished in the current study.					
37175077	7	75	theme	growth	1176:1181	arg1	consistent					1187:1196	consistent	1187:1196	consistent	1187:1196	The results of the cell aggregation and hyphal growth are consistent with the antibiofilm activity that is found in the treatment group.					
37175077	7	75	theme	growth	1176:1181	arg1	results					1133:1139	The results	1129:1139	The results of the cell aggregation and hyphal growth	1129:1181	The results of the cell aggregation and hyphal growth are consistent with the antibiofilm activity that is found in the treatment group.					
37175077	4	76	with	interaction	811:821	arg1	MOFs					846:849	the β-CD-K MOFs	835:849	the β-CD-K MOFs	835:849	The shifting in the chemical shift values of QRC in the MOFs may be the reason for the interaction of QRC with the β-CD-K MOFs.					
37358033	7	0	theme	intracellular	1141:1153	arg1	glucose					1155:1161	intracellular glucose	1141:1161	intracellular glucose	1141:1161	When endocytosed by tumor cells, intracellular glucose was oxidized to H2O2 and gluconic acid catalyzed by immobilized GOx of HGPF.					
37358033	6	1	used	used	957:960	arg2	platform					967:974	a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA)	965:1058	a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA)	965:1058	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	1	used	used	957:960	arg2	Fe					949:950	Fe	949:950	Fe	949:950	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	1	used	used	957:960	arg2	PCN-224					941:947	the POD-like nanozyme PCN-224	919:947	the POD-like nanozyme PCN-224(Fe)	919:951	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	11	2	theme	oxygen	1672:1677	arg1	sources					1679:1685	intracellular oxygen sources	1658:1685	intracellular oxygen sources	1658:1685	HGPF was expected to integrate intracellular oxygen sources and overcome the dilemma of limited intracellular H2O2 content.					
37358033	2	3	theme	cellular	345:352	arg1	strategy					403:410	a drug-free strategy	391:410	a drug-free strategy to efficiently induce apoptosis in tumor cells	391:457	Amplifying cellular oxidative stress is considered to be a drug-free strategy to efficiently induce apoptosis in tumor cells.					
37358033	2	3	theme	cellular	345:352	arg1	stress					364:369	Amplifying cellular oxidative stress	334:369	Amplifying cellular oxidative stress	334:369	Amplifying cellular oxidative stress is considered to be a drug-free strategy to efficiently induce apoptosis in tumor cells.					
37358033	1	4	theme	species	256:262	arg1	generation					226:235	the generation	222:235	the generation of reactive oxygen species (ROS)	222:268	Peroxidase (POD)-like nanozymes have been found to act as nanoreactors for the generation of reactive oxygen species (ROS) to resolve drug resistance in the tumor microenvironment (TME).					
37358033	0	5	theme	synergistic	91:101	arg1	amplification					103:115	synergistic amplification	91:115	synergistic amplification of cellular oxidative stress	91:144	A porphyrin-MOF-based integrated nanozyme system for catalytic cascades and light-enhanced synergistic amplification of cellular oxidative stress.					
37358033	6	6	dep	platform	967:974	arg1	immobilize					979:988	immobilize	979:988	to immobilize glucose oxidase (GOx)	976:1010	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	6	dep	platform	967:974	arg1	embedded					1025:1032	embedded	1025:1032	embedded	1025:1032	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	12	7	theme	catalytic	1856:1864	arg1	cascades					1876:1883	light-enhanced catalytic oxidation cascades	1841:1883	light-enhanced catalytic oxidation cascades	1841:1883	Consequently, HGPF was constructed as an integrated nanoreactor to simultaneously achieve light-enhanced catalytic oxidation cascades, providing a promising strategy for a synergistic amplification of cellular oxidative stress.					
37358033	2	8	theme	Amplifying	334:343	arg1	strategy					403:410	a drug-free strategy	391:410	a drug-free strategy to efficiently induce apoptosis in tumor cells	391:457	Amplifying cellular oxidative stress is considered to be a drug-free strategy to efficiently induce apoptosis in tumor cells.					
37358033	2	8	theme	Amplifying	334:343	arg1	stress					364:369	Amplifying cellular oxidative stress	334:369	Amplifying cellular oxidative stress	334:369	Amplifying cellular oxidative stress is considered to be a drug-free strategy to efficiently induce apoptosis in tumor cells.					
37358033	12	9	theme	oxidation	1866:1874	arg1	cascades					1876:1883	light-enhanced catalytic oxidation cascades	1841:1883	light-enhanced catalytic oxidation cascades	1841:1883	Consequently, HGPF was constructed as an integrated nanoreactor to simultaneously achieve light-enhanced catalytic oxidation cascades, providing a promising strategy for a synergistic amplification of cellular oxidative stress.					
37358033	0	10	theme	cellular	120:127	arg1	stress					139:144	cellular oxidative stress	120:144	cellular oxidative stress	120:144	A porphyrin-MOF-based integrated nanozyme system for catalytic cascades and light-enhanced synergistic amplification of cellular oxidative stress.					
37358033	9	11	theme	HGPF	1433:1436	arg1	iron-porphyrin					1415:1428	the iron-porphyrin	1411:1428	the iron-porphyrin of HGPF	1411:1436	Under light irradiation, the iron-porphyrin of HGPF acted as a photosensitizer to facilely produce singlet oxygen (1O2).					
37358033	9	11	theme	HGPF	1433:1436	arg1	photosensitizer					1449:1463	a photosensitizer	1447:1463	a photosensitizer	1447:1463	Under light irradiation, the iron-porphyrin of HGPF acted as a photosensitizer to facilely produce singlet oxygen (1O2).					
37358033	10	12	from	apoptosis	1601:1609	arg1	cells					1620:1624	tumor cells	1614:1624	tumor cells	1614:1624	Such a synergistic generation of ROS strikingly amplified oxidative stress and induced severe apoptosis in tumor cells.					
37358033	4	13	theme	extra	752:756	arg1	cytotoxicity					758:769	extra cytotoxicity	752:769	extra cytotoxicity	752:769	Moreover, additional operational processes combined with exogenous reagents to achieve oxidative stress lead to a dilemma of extra cytotoxicity.					
37358033	7	14	theme	tumor	1128:1132	arg1	cells					1134:1138	tumor cells	1128:1138	tumor cells	1128:1138	When endocytosed by tumor cells, intracellular glucose was oxidized to H2O2 and gluconic acid catalyzed by immobilized GOx of HGPF.					
37358033	8	15	theme	heme	1264:1267	arg1	analogs					1269:1275	heme analogs	1264:1275	heme analogs	1264:1275	Afterwards, inspired by heme analogs, H2O2 was catalyzed by iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH).					
37358033	4	16	theme	oxidative	714:722	arg1	stress					724:729	oxidative stress	714:729	oxidative stress	714:729	Moreover, additional operational processes combined with exogenous reagents to achieve oxidative stress lead to a dilemma of extra cytotoxicity.					
37358033	0	17	theme	stress	139:144	arg1	amplification					103:115	synergistic amplification	91:115	synergistic amplification of cellular oxidative stress	91:144	A porphyrin-MOF-based integrated nanozyme system for catalytic cascades and light-enhanced synergistic amplification of cellular oxidative stress.					
37358033	10	18	theme	oxidative	1565:1573	arg1	stress					1575:1580	oxidative stress	1565:1580	oxidative stress	1565:1580	Such a synergistic generation of ROS strikingly amplified oxidative stress and induced severe apoptosis in tumor cells.					
37358033	0	19	theme	oxidative	129:137	arg1	stress					139:144	cellular oxidative stress	120:144	cellular oxidative stress	120:144	A porphyrin-MOF-based integrated nanozyme system for catalytic cascades and light-enhanced synergistic amplification of cellular oxidative stress.					
37358033	1	20	from	resistance	286:295	arg1	TME					328:330	TME	328:330	TME	328:330	Peroxidase (POD)-like nanozymes have been found to act as nanoreactors for the generation of reactive oxygen species (ROS) to resolve drug resistance in the tumor microenvironment (TME).					
37358033	1	20	from	resistance	286:295	arg1	microenvironment					310:325	the tumor microenvironment	300:325	the tumor microenvironment (TME)	300:331	Peroxidase (POD)-like nanozymes have been found to act as nanoreactors for the generation of reactive oxygen species (ROS) to resolve drug resistance in the tumor microenvironment (TME).					
37358033	6	21	theme	cells	1101:1105	arg1	ability					1084:1090	the targeting ability	1070:1090	the targeting ability of tumor cells	1070:1105	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	22	theme	hyaluronic	1039:1048	arg1	HA					1056:1057	HA	1056:1057	HA	1056:1057	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	22	theme	hyaluronic	1039:1048	arg1	acid					1050:1053	hyaluronic acid	1039:1053	hyaluronic acid (HA)	1039:1058	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	23	theme	POD-like	923:930	arg1	Fe					949:950	Fe	949:950	Fe	949:950	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	23	theme	POD-like	923:930	arg1	PCN-224					941:947	the POD-like nanozyme PCN-224	919:947	the POD-like nanozyme PCN-224(Fe)	919:951	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	23	theme	POD-like	923:930	arg1	platform					967:974	a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA)	965:1058	a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA)	965:1058	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	12	24	theme	cellular	1952:1959	arg1	stress					1971:1976	cellular oxidative stress	1952:1976	cellular oxidative stress	1952:1976	Consequently, HGPF was constructed as an integrated nanoreactor to simultaneously achieve light-enhanced catalytic oxidation cascades, providing a promising strategy for a synergistic amplification of cellular oxidative stress.					
37358033	12	25	theme	light-enhanced	1841:1854	arg1	cascades					1876:1883	light-enhanced catalytic oxidation cascades	1841:1883	light-enhanced catalytic oxidation cascades	1841:1883	Consequently, HGPF was constructed as an integrated nanoreactor to simultaneously achieve light-enhanced catalytic oxidation cascades, providing a promising strategy for a synergistic amplification of cellular oxidative stress.					
37358033	12	26	theme	synergistic	1923:1933	arg1	amplification					1935:1947	a synergistic amplification	1921:1947	a synergistic amplification of cellular oxidative stress	1921:1976	Consequently, HGPF was constructed as an integrated nanoreactor to simultaneously achieve light-enhanced catalytic oxidation cascades, providing a promising strategy for a synergistic amplification of cellular oxidative stress.					
37358033	6	27	theme	tumor	1095:1099	arg1	cells					1101:1105	tumor cells	1095:1105	tumor cells	1095:1105	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	11	28	theme	content	1742:1748	arg1	dilemma					1704:1710	the dilemma	1700:1710	the dilemma of limited intracellular H2O2 content	1700:1748	HGPF was expected to integrate intracellular oxygen sources and overcome the dilemma of limited intracellular H2O2 content.					
37358033	4	29	theme	exogenous	684:692	arg1	reagents					694:701	exogenous reagents	684:701	exogenous reagents	684:701	Moreover, additional operational processes combined with exogenous reagents to achieve oxidative stress lead to a dilemma of extra cytotoxicity.					
37358033	5	30	theme	HA	842:843	arg1	Fe					857:858	Fe	857:858	Fe	857:858	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	5	30	theme	HA	842:843	arg1	HGPF					862:865	HGPF	862:865	HGPF	862:865	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	5	30	theme	HA	842:843	arg1	PCN-224					849:855	HA@GOx@PCN-224	842:855	HA@GOx@PCN-224(Fe) (HGPF)	842:866	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	11	31	theme	limited	1715:1721	arg1	content					1742:1748	limited intracellular H2O2 content	1715:1748	limited intracellular H2O2 content	1715:1748	HGPF was expected to integrate intracellular oxygen sources and overcome the dilemma of limited intracellular H2O2 content.					
37358033	9	32	theme	singlet	1485:1491	arg1	1O2					1501:1503	1O2	1501:1503	1O2	1501:1503	Under light irradiation, the iron-porphyrin of HGPF acted as a photosensitizer to facilely produce singlet oxygen (1O2).					
37358033	9	32	theme	singlet	1485:1491	arg1	oxygen					1493:1498	singlet oxygen	1485:1498	singlet oxygen (1O2)	1485:1504	Under light irradiation, the iron-porphyrin of HGPF acted as a photosensitizer to facilely produce singlet oxygen (1O2).					
37358033	8	33	theme	hydroxyl	1361:1368	arg1	˙OH					1380:1382	˙OH	1380:1382	˙OH	1380:1382	Afterwards, inspired by heme analogs, H2O2 was catalyzed by iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH).					
37358033	8	33	theme	hydroxyl	1361:1368	arg1	radicals					1370:1377	hydroxyl radicals	1361:1377	hydroxyl radicals (˙OH)	1361:1383	Afterwards, inspired by heme analogs, H2O2 was catalyzed by iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH).					
37358033	0	34	theme	nanozyme	33:40	arg1	system					42:47	nanozyme system	33:47	nanozyme system for catalytic cascades	33:70	A porphyrin-MOF-based integrated nanozyme system for catalytic cascades and light-enhanced synergistic amplification of cellular oxidative stress.					
37358033	10	35	theme	severe	1594:1599	arg1	apoptosis					1601:1609	severe apoptosis	1594:1609	severe apoptosis in tumor cells	1594:1624	Such a synergistic generation of ROS strikingly amplified oxidative stress and induced severe apoptosis in tumor cells.					
37358033	4	36	theme	cytotoxicity	758:769	arg1	dilemma					741:747	a dilemma	739:747	a dilemma of extra cytotoxicity	739:769	Moreover, additional operational processes combined with exogenous reagents to achieve oxidative stress lead to a dilemma of extra cytotoxicity.					
37358033	5	37	theme	@	848:848	arg1	Fe					857:858	Fe	857:858	Fe	857:858	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	5	37	theme	@	848:848	arg1	HGPF					862:865	HGPF	862:865	HGPF	862:865	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	5	37	theme	@	848:848	arg1	PCN-224					849:855	HA@GOx@PCN-224	842:855	HA@GOx@PCN-224(Fe) (HGPF)	842:866	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	5	38	theme	GOx	845:847	arg1	Fe					857:858	Fe	857:858	Fe	857:858	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	5	38	theme	GOx	845:847	arg1	HGPF					862:865	HGPF	862:865	HGPF	862:865	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	5	38	theme	GOx	845:847	arg1	PCN-224					849:855	HA@GOx@PCN-224	842:855	HA@GOx@PCN-224(Fe) (HGPF)	842:866	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	1	39	theme	drug	281:284	arg1	resistance					286:295	drug resistance	281:295	drug resistance in the tumor microenvironment (TME)	281:331	Peroxidase (POD)-like nanozymes have been found to act as nanoreactors for the generation of reactive oxygen species (ROS) to resolve drug resistance in the tumor microenvironment (TME).					
37358033	3	40	theme	intracellular	492:504	arg1	H2O2					525:528	H2O2	525:528	H2O2	525:528	However, the limited content of intracellular hydrogen peroxide (H2O2) extremely restricts the performance of POD-like nanozymes to amplify cellular oxidative stress.					
37358033	3	40	theme	intracellular	492:504	arg1	peroxide					515:522	intracellular hydrogen peroxide	492:522	intracellular hydrogen peroxide (H2O2)	492:529	However, the limited content of intracellular hydrogen peroxide (H2O2) extremely restricts the performance of POD-like nanozymes to amplify cellular oxidative stress.					
37358033	5	41	theme	integrated	781:790	arg1	composite					826:834	an integrated iron-porphyrin-MOF-based nanozyme composite	778:834	an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF)	778:866	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	10	42	theme	tumor	1614:1618	arg1	cells					1620:1624	tumor cells	1614:1624	tumor cells	1614:1624	Such a synergistic generation of ROS strikingly amplified oxidative stress and induced severe apoptosis in tumor cells.					
37358033	4	43	theme	operational	648:658	arg1	processes					660:668	additional operational processes	637:668	additional operational processes combined with exogenous reagents to achieve oxidative stress	637:729	Moreover, additional operational processes combined with exogenous reagents to achieve oxidative stress lead to a dilemma of extra cytotoxicity.					
37358033	12	44	theme	promising	1898:1906	arg1	strategy					1908:1915	a promising strategy	1896:1915	a promising strategy for a synergistic amplification of cellular oxidative stress	1896:1976	Consequently, HGPF was constructed as an integrated nanoreactor to simultaneously achieve light-enhanced catalytic oxidation cascades, providing a promising strategy for a synergistic amplification of cellular oxidative stress.					
37358033	10	45	theme	ROS	1540:1542	arg1	generation					1526:1535	Such a synergistic generation	1507:1535	Such a synergistic generation of ROS	1507:1542	Such a synergistic generation of ROS strikingly amplified oxidative stress and induced severe apoptosis in tumor cells.					
37358033	2	46	theme	drug-free	393:401	arg1	strategy					403:410	a drug-free strategy	391:410	a drug-free strategy to efficiently induce apoptosis in tumor cells	391:457	Amplifying cellular oxidative stress is considered to be a drug-free strategy to efficiently induce apoptosis in tumor cells.					
37358033	2	46	theme	drug-free	393:401	arg1	stress					364:369	Amplifying cellular oxidative stress	334:369	Amplifying cellular oxidative stress	334:369	Amplifying cellular oxidative stress is considered to be a drug-free strategy to efficiently induce apoptosis in tumor cells.					
37358033	5	47	theme	iron-porphyrin-MOF-based	792:815	arg1	composite					826:834	an integrated iron-porphyrin-MOF-based nanozyme composite	778:834	an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF)	778:866	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	12	48	theme	integrated	1792:1801	arg1	HGPF					1765:1768	HGPF	1765:1768	HGPF	1765:1768	Consequently, HGPF was constructed as an integrated nanoreactor to simultaneously achieve light-enhanced catalytic oxidation cascades, providing a promising strategy for a synergistic amplification of cellular oxidative stress.					
37358033	12	48	theme	integrated	1792:1801	arg1	nanoreactor					1803:1813	an integrated nanoreactor	1789:1813	an integrated nanoreactor to simultaneously achieve light-enhanced catalytic oxidation cascades	1789:1883	Consequently, HGPF was constructed as an integrated nanoreactor to simultaneously achieve light-enhanced catalytic oxidation cascades, providing a promising strategy for a synergistic amplification of cellular oxidative stress.					
37358033	5	49	theme	@	844:844	arg1	Fe					857:858	Fe	857:858	Fe	857:858	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	5	49	theme	@	844:844	arg1	HGPF					862:865	HGPF	862:865	HGPF	862:865	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	5	49	theme	@	844:844	arg1	PCN-224					849:855	HA@GOx@PCN-224	842:855	HA@GOx@PCN-224(Fe) (HGPF)	842:866	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	12	50	theme	oxidative	1961:1969	arg1	stress					1971:1976	cellular oxidative stress	1952:1976	cellular oxidative stress	1952:1976	Consequently, HGPF was constructed as an integrated nanoreactor to simultaneously achieve light-enhanced catalytic oxidation cascades, providing a promising strategy for a synergistic amplification of cellular oxidative stress.					
37358033	4	51	theme	additional	637:646	arg1	processes					660:668	additional operational processes	637:668	additional operational processes combined with exogenous reagents to achieve oxidative stress	637:729	Moreover, additional operational processes combined with exogenous reagents to achieve oxidative stress lead to a dilemma of extra cytotoxicity.					
37358033	5	52	theme	nanozyme	817:824	arg1	composite					826:834	an integrated iron-porphyrin-MOF-based nanozyme composite	778:834	an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF)	778:866	Here, an integrated iron-porphyrin-MOF-based nanozyme composite named HA@GOx@PCN-224(Fe) (HGPF) was precisely designed and constructed.					
37358033	6	53	theme	nanozyme	932:939	arg1	Fe					949:950	Fe	949:950	Fe	949:950	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	53	theme	nanozyme	932:939	arg1	PCN-224					941:947	the POD-like nanozyme PCN-224	919:947	the POD-like nanozyme PCN-224(Fe)	919:951	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	53	theme	nanozyme	932:939	arg1	platform					967:974	a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA)	965:1058	a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA)	965:1058	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	54	with	embedded	1025:1032	arg1	HA					1056:1057	HA	1056:1057	HA	1056:1057	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	54	with	embedded	1025:1032	arg1	acid					1050:1053	hyaluronic acid	1039:1053	hyaluronic acid (HA)	1039:1058	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	3	55	theme	POD-like	570:577	arg1	nanozymes					579:587	POD-like nanozymes	570:587	POD-like nanozymes	570:587	However, the limited content of intracellular hydrogen peroxide (H2O2) extremely restricts the performance of POD-like nanozymes to amplify cellular oxidative stress.					
37358033	3	56	theme	limited	473:479	arg1	content					481:487	the limited content	469:487	the limited content of intracellular hydrogen peroxide (H2O2)	469:529	However, the limited content of intracellular hydrogen peroxide (H2O2) extremely restricts the performance of POD-like nanozymes to amplify cellular oxidative stress.					
37358033	0	57	theme	catalytic	53:61	arg1	cascades					63:70	catalytic cascades	53:70	catalytic cascades	53:70	A porphyrin-MOF-based integrated nanozyme system for catalytic cascades and light-enhanced synergistic amplification of cellular oxidative stress.					
37358033	1	58	theme	tumor	304:308	arg1	TME					328:330	TME	328:330	TME	328:330	Peroxidase (POD)-like nanozymes have been found to act as nanoreactors for the generation of reactive oxygen species (ROS) to resolve drug resistance in the tumor microenvironment (TME).					
37358033	1	58	theme	tumor	304:308	arg1	microenvironment					310:325	the tumor microenvironment	300:325	the tumor microenvironment (TME)	300:331	Peroxidase (POD)-like nanozymes have been found to act as nanoreactors for the generation of reactive oxygen species (ROS) to resolve drug resistance in the tumor microenvironment (TME).					
37358033	2	59	theme	tumor	447:451	arg1	cells					453:457	tumor cells	447:457	tumor cells	447:457	Amplifying cellular oxidative stress is considered to be a drug-free strategy to efficiently induce apoptosis in tumor cells.					
37358033	3	60	theme	nanozymes	579:587	arg1	performance					555:565	the performance	551:565	the performance of POD-like nanozymes	551:587	However, the limited content of intracellular hydrogen peroxide (H2O2) extremely restricts the performance of POD-like nanozymes to amplify cellular oxidative stress.					
37358033	7	61	theme	immobilized	1215:1225	arg1	GOx					1227:1229	immobilized GOx	1215:1229	immobilized GOx of HGPF	1215:1237	When endocytosed by tumor cells, intracellular glucose was oxidized to H2O2 and gluconic acid catalyzed by immobilized GOx of HGPF.					
37358033	7	62	theme	gluconic	1188:1195	arg1	acid					1197:1200	gluconic acid	1188:1200	gluconic acid	1188:1200	When endocytosed by tumor cells, intracellular glucose was oxidized to H2O2 and gluconic acid catalyzed by immobilized GOx of HGPF.					
37358033	8	63	theme	nanozyme	1340:1347	arg1	nanozyme					1340:1347	the HGPF nanozyme	1331:1347	the HGPF nanozyme to generate hydroxyl radicals (˙OH)	1331:1383	Afterwards, inspired by heme analogs, H2O2 was catalyzed by iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH).					
37358033	8	63	theme	nanozyme	1340:1347	arg1	sites					1322:1326	iron-porphyrin active sites	1300:1326	iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH)	1300:1383	Afterwards, inspired by heme analogs, H2O2 was catalyzed by iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH).					
37358033	8	64	theme	active	1315:1320	arg1	nanozyme					1340:1347	the HGPF nanozyme	1331:1347	the HGPF nanozyme to generate hydroxyl radicals (˙OH)	1331:1383	Afterwards, inspired by heme analogs, H2O2 was catalyzed by iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH).					
37358033	8	64	theme	active	1315:1320	arg1	sites					1322:1326	iron-porphyrin active sites	1300:1326	iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH)	1300:1383	Afterwards, inspired by heme analogs, H2O2 was catalyzed by iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH).					
37358033	8	65	theme	iron-porphyrin	1300:1313	arg1	nanozyme					1340:1347	the HGPF nanozyme	1331:1347	the HGPF nanozyme to generate hydroxyl radicals (˙OH)	1331:1383	Afterwards, inspired by heme analogs, H2O2 was catalyzed by iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH).					
37358033	8	65	theme	iron-porphyrin	1300:1313	arg1	sites					1322:1326	iron-porphyrin active sites	1300:1326	iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH)	1300:1383	Afterwards, inspired by heme analogs, H2O2 was catalyzed by iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH).					
37358033	11	66	theme	intracellular	1658:1670	arg1	sources					1679:1685	intracellular oxygen sources	1658:1685	intracellular oxygen sources	1658:1685	HGPF was expected to integrate intracellular oxygen sources and overcome the dilemma of limited intracellular H2O2 content.					
37358033	12	67	theme	stress	1971:1976	arg1	amplification					1935:1947	a synergistic amplification	1921:1947	a synergistic amplification of cellular oxidative stress	1921:1976	Consequently, HGPF was constructed as an integrated nanoreactor to simultaneously achieve light-enhanced catalytic oxidation cascades, providing a promising strategy for a synergistic amplification of cellular oxidative stress.					
37358033	8	68	theme	HGPF	1335:1338	arg1	nanozyme					1340:1347	the HGPF nanozyme	1331:1347	the HGPF nanozyme to generate hydroxyl radicals (˙OH)	1331:1383	Afterwards, inspired by heme analogs, H2O2 was catalyzed by iron-porphyrin active sites of the HGPF nanozyme to generate hydroxyl radicals (˙OH).					
37358033	11	69	theme	intracellular	1723:1735	arg1	content					1742:1748	limited intracellular H2O2 content	1715:1748	limited intracellular H2O2 content	1715:1748	HGPF was expected to integrate intracellular oxygen sources and overcome the dilemma of limited intracellular H2O2 content.					
37358033	3	70	theme	peroxide	515:522	arg1	content					481:487	the limited content	469:487	the limited content of intracellular hydrogen peroxide (H2O2)	469:529	However, the limited content of intracellular hydrogen peroxide (H2O2) extremely restricts the performance of POD-like nanozymes to amplify cellular oxidative stress.					
37358033	11	71	theme	H2O2	1737:1740	arg1	content					1742:1748	limited intracellular H2O2 content	1715:1748	limited intracellular H2O2 content	1715:1748	HGPF was expected to integrate intracellular oxygen sources and overcome the dilemma of limited intracellular H2O2 content.					
37358033	6	72	theme	glucose	990:996	arg1	GOx					1007:1009	GOx	1007:1009	GOx	1007:1009	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	6	72	theme	glucose	990:996	arg1	oxidase					998:1004	glucose oxidase	990:1004	glucose oxidase (GOx)	990:1010	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	9	73	theme	light	1392:1396	arg1	irradiation					1398:1408	light irradiation	1392:1408	light irradiation	1392:1408	Under light irradiation, the iron-porphyrin of HGPF acted as a photosensitizer to facilely produce singlet oxygen (1O2).					
37358033	3	74	theme	cellular	600:607	arg1	stress					619:624	cellular oxidative stress	600:624	cellular oxidative stress	600:624	However, the limited content of intracellular hydrogen peroxide (H2O2) extremely restricts the performance of POD-like nanozymes to amplify cellular oxidative stress.					
37358033	1	75	theme	-like	163:167	arg1	nanoreactors					205:216	nanoreactors	205:216	nanoreactors for the generation of reactive oxygen species (ROS)	205:268	Peroxidase (POD)-like nanozymes have been found to act as nanoreactors for the generation of reactive oxygen species (ROS) to resolve drug resistance in the tumor microenvironment (TME).					
37358033	1	75	theme	-like	163:167	arg1	nanozymes					169:177	Peroxidase (POD)-like nanozymes	147:177	Peroxidase (POD)-like nanozymes	147:177	Peroxidase (POD)-like nanozymes have been found to act as nanoreactors for the generation of reactive oxygen species (ROS) to resolve drug resistance in the tumor microenvironment (TME).					
37358033	3	76	theme	hydrogen	506:513	arg1	H2O2					525:528	H2O2	525:528	H2O2	525:528	However, the limited content of intracellular hydrogen peroxide (H2O2) extremely restricts the performance of POD-like nanozymes to amplify cellular oxidative stress.					
37358033	3	76	theme	hydrogen	506:513	arg1	peroxide					515:522	intracellular hydrogen peroxide	492:522	intracellular hydrogen peroxide (H2O2)	492:529	However, the limited content of intracellular hydrogen peroxide (H2O2) extremely restricts the performance of POD-like nanozymes to amplify cellular oxidative stress.					
37358033	7	77	theme	HGPF	1234:1237	arg1	GOx					1227:1229	immobilized GOx	1215:1229	immobilized GOx of HGPF	1215:1237	When endocytosed by tumor cells, intracellular glucose was oxidized to H2O2 and gluconic acid catalyzed by immobilized GOx of HGPF.					
37358033	1	78	theme	reactive	240:247	arg1	ROS					265:267	ROS	265:267	ROS	265:267	Peroxidase (POD)-like nanozymes have been found to act as nanoreactors for the generation of reactive oxygen species (ROS) to resolve drug resistance in the tumor microenvironment (TME).					
37358033	1	78	theme	reactive	240:247	arg1	species					256:262	reactive oxygen species	240:262	reactive oxygen species (ROS)	240:268	Peroxidase (POD)-like nanozymes have been found to act as nanoreactors for the generation of reactive oxygen species (ROS) to resolve drug resistance in the tumor microenvironment (TME).					
37358033	10	79	theme	synergistic	1514:1524	arg1	generation					1526:1535	Such a synergistic generation	1507:1535	Such a synergistic generation of ROS	1507:1542	Such a synergistic generation of ROS strikingly amplified oxidative stress and induced severe apoptosis in tumor cells.					
37358033	6	80	theme	targeting	1074:1082	arg1	ability					1084:1090	the targeting ability	1070:1090	the targeting ability of tumor cells	1070:1105	Generally, the POD-like nanozyme PCN-224(Fe) was used as a platform to immobilize glucose oxidase (GOx), and further embedded with hyaluronic acid (HA) to enable the targeting ability of tumor cells.					
37358033	3	81	theme	oxidative	609:617	arg1	stress					619:624	cellular oxidative stress	600:624	cellular oxidative stress	600:624	However, the limited content of intracellular hydrogen peroxide (H2O2) extremely restricts the performance of POD-like nanozymes to amplify cellular oxidative stress.					
37358033	2	82	theme	oxidative	354:362	arg1	strategy					403:410	a drug-free strategy	391:410	a drug-free strategy to efficiently induce apoptosis in tumor cells	391:457	Amplifying cellular oxidative stress is considered to be a drug-free strategy to efficiently induce apoptosis in tumor cells.					
37358033	2	82	theme	oxidative	354:362	arg1	stress					364:369	Amplifying cellular oxidative stress	334:369	Amplifying cellular oxidative stress	334:369	Amplifying cellular oxidative stress is considered to be a drug-free strategy to efficiently induce apoptosis in tumor cells.					
37358033	1	83	theme	oxygen	249:254	arg1	ROS					265:267	ROS	265:267	ROS	265:267	Peroxidase (POD)-like nanozymes have been found to act as nanoreactors for the generation of reactive oxygen species (ROS) to resolve drug resistance in the tumor microenvironment (TME).					
37358033	1	83	theme	oxygen	249:254	arg1	species					256:262	reactive oxygen species	240:262	reactive oxygen species (ROS)	240:268	Peroxidase (POD)-like nanozymes have been found to act as nanoreactors for the generation of reactive oxygen species (ROS) to resolve drug resistance in the tumor microenvironment (TME).					
35130096	9	0	dep	significantly	1080:1092	arg1	p < 0.05					1095:1102	p < 0.05	1095:1102	p < 0.05	1095:1102	Both coagulant dosage and pH significantly (p < 0.05) affect the flocculation efficiency of Botryococcus sp.					
35130096	5	1	dep	alum	636:639	arg1	dosage					654:659	dosage	654:659	dosage	654:659	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	7	2	theme	phosphorus	883:892	arg1	removal					804:810	the highest removal	792:810	the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP)	792:897	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	13	3	theme	biomass	1548:1554	arg1	optimization					1567:1578	biomass harvesting optimization	1548:1578	biomass harvesting optimization using a flocculation technique	1548:1609	shows a great removal efficiency of FPW contamination, whereas RSM provides excellent analysis for biomass harvesting optimization using a flocculation technique.					
35130096	11	4	theme	chitosan	1361:1368	arg1	dosage					1370:1375	optimum chitosan dosage	1353:1375	optimum chitosan dosage	1353:1375	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	2	5	theme	Microalgae	168:177	arg1	method					222:227	a sustainable method	208:227	a sustainable method for eco-friendly wastewater phycoremediation and valuable biomass production	208:304	Microalgae cultivation is well known as a sustainable method for eco-friendly wastewater phycoremediation and valuable biomass production.					
35130096	2	5	theme	Microalgae	168:177	arg1	cultivation					179:189	Microalgae cultivation	168:189	Microalgae cultivation	168:189	Microalgae cultivation is well known as a sustainable method for eco-friendly wastewater phycoremediation and valuable biomass production.					
35130096	5	6	theme	sensitivity	668:678	arg1	effects					625:631	the effects	621:631	the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency	621:705	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	5	7	theme	central	564:570	arg1	FCCCD					590:594	FCCCD	590:594	FCCCD	590:594	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	5	7	theme	central	564:570	arg1	design					582:587	face-centered central composite design	550:587	face-centered central composite design (FCCCD)	550:595	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	5	8	theme	flocculation	683:694	arg1	efficiency					696:705	flocculation efficiency	683:705	flocculation efficiency	683:705	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	8	9	theme	second-order	977:988	arg1	function					1001:1008	A second-order polynomial function	975:1008	A second-order polynomial function	975:1008	A second-order polynomial function fits well with the experimental results.					
35130096	11	10	theme	highest	1191:1197	arg1	efficiency					1212:1221	The highest flocculation efficiency	1187:1221	The highest flocculation efficiency (92.4%)	1187:1229	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	11	10	theme	highest	1191:1197	arg1	%					1228:1228	92.4%	1224:1228	92.4%	1224:1228	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	7	11	theme	organic	851:857	arg1	TOC					867:869	TOC	867:869	TOC	867:869	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	7	11	theme	organic	851:857	arg1	carbon					859:864	total organic carbon	845:864	total organic carbon (TOC)	845:870	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	13	12	dep	shows	1449:1453	arg1	whereas					1504:1510	whereas	1504:1510	whereas	1504:1510	shows a great removal efficiency of FPW contamination, whereas RSM provides excellent analysis for biomass harvesting optimization using a flocculation technique.					
35130096	11	13	theme	flocculation	1311:1322	arg1	efficiency					1324:1333	94.9% flocculation efficiency	1305:1333	94.9% flocculation efficiency	1305:1333	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	13	14	theme	contamination	1489:1501	arg1	efficiency					1471:1480	a great removal efficiency	1455:1480	a great removal efficiency of FPW contamination	1455:1501	shows a great removal efficiency of FPW contamination, whereas RSM provides excellent analysis for biomass harvesting optimization using a flocculation technique.					
35130096	11	15	theme	94.9	1305:1308	arg1	%					1309:1309	%	1309:1309	%	1309:1309	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	2	16	theme	wastewater	246:255	arg1	phycoremediation					257:272	eco-friendly wastewater phycoremediation	233:272	eco-friendly wastewater phycoremediation	233:272	Microalgae cultivation is well known as a sustainable method for eco-friendly wastewater phycoremediation and valuable biomass production.					
35130096	13	17	theme	removal	1463:1469	arg1	efficiency					1471:1480	a great removal efficiency	1455:1480	a great removal efficiency of FPW contamination	1455:1501	shows a great removal efficiency of FPW contamination, whereas RSM provides excellent analysis for biomass harvesting optimization using a flocculation technique.					
35130096	5	18	from	effects	625:631	arg1	efficiency					696:705	flocculation efficiency	683:705	flocculation efficiency	683:705	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	0	19	theme	Botryococcus	75:86	arg1	sp					88:89	microalgae Botryococcus sp	64:89	microalgae Botryococcus sp	64:89	Outdoor phycoremediation and biomass harvesting optimization of microalgae Botryococcus sp.					
35130096	2	20	theme	biomass	287:293	arg1	production					295:304	valuable biomass production	278:304	valuable biomass production	278:304	Microalgae cultivation is well known as a sustainable method for eco-friendly wastewater phycoremediation and valuable biomass production.					
35130096	3	21	theme	organic	370:376	arg1	compounds					378:386	organic compounds	370:386	organic compounds	370:386	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	3	22	theme	processing	412:421	arg1	FPW					435:437	FPW	435:437	FPW	435:437	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	3	22	theme	processing	412:421	arg1	wastewater					423:432	food processing wastewater	407:432	food processing wastewater (FPW) using Botryococcus sp	407:460	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	11	23	theme	pH	1273:1274	arg1	dosage					1249:1254	a dosage	1247:1254	a dosage of 166 mg L-1and pH 12	1247:1277	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	9	24	theme	coagulant	1056:1064	arg1	dosage					1066:1071	coagulant dosage	1056:1071	coagulant dosage	1056:1071	Both coagulant dosage and pH significantly (p < 0.05) affect the flocculation efficiency of Botryococcus sp.					
35130096	7	25	theme	%	940:940	arg1	phycoremediation					916:931	phycoremediation	916:931	phycoremediation of 96.1%, 87.2%, and 35.4%, respectively	916:972	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	11	26	theme	166 mg	1259:1264	arg1	pH					1273:1274	166 mg L-1and pH 12	1259:1277	166 mg L-1and pH 12	1259:1277	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	6	27	theme	sp	748:749	arg1	rate					727:730	The maximum growth rate	708:730	The maximum growth rate of Botryococcus sp	708:749	The maximum growth rate of Botryococcus sp.					
35130096	0	28	theme	Outdoor	0:6	arg1	phycoremediation					8:23	Outdoor phycoremediation	0:23	Outdoor phycoremediation	0:23	Outdoor phycoremediation and biomass harvesting optimization of microalgae Botryococcus sp.					
35130096	1	29	theme	food	106:109	arg1	wastewater					122:131	food processing wastewater	106:131	food processing wastewater using an enclosed photobioreactor	106:165	cultivated in food processing wastewater using an enclosed photobioreactor.					
35130096	7	30	theme	total	877:881	arg1	TP					895:896	TP	895:896	TP	895:896	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	7	30	theme	total	877:881	arg1	phosphorus					883:892	total phosphorus	877:892	total phosphorus (TP)	877:897	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	11	31	theme	flocculation	1199:1210	arg1	efficiency					1212:1221	The highest flocculation efficiency	1187:1221	The highest flocculation efficiency (92.4%)	1187:1229	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	11	31	theme	flocculation	1199:1210	arg1	%					1228:1228	92.4%	1224:1228	92.4%	1224:1228	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	6	32	theme	growth	720:725	arg1	rate					727:730	The maximum growth rate	708:730	The maximum growth rate of Botryococcus sp	708:749	The maximum growth rate of Botryococcus sp.					
35130096	7	33	theme	demand	831:836	arg1	removal					804:810	the highest removal	792:810	the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP)	792:897	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	7	34	dep	removal	804:810	arg1	12 days					905:911	12 days	905:911	12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively	905:972	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	3	35	theme	Botryococcus	446:457	arg1	sp					459:460	Botryococcus sp	446:460	Botryococcus sp	446:460	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	7	36	theme	chemical	815:822	arg1	demand					831:836	chemical oxygen demand	815:836	chemical oxygen demand (COD)	815:842	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	7	36	theme	chemical	815:822	arg1	COD					839:841	COD	839:841	COD	839:841	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	13	37	theme	flocculation	1588:1599	arg1	technique					1601:1609	a flocculation technique	1586:1609	a flocculation technique	1586:1609	shows a great removal efficiency of FPW contamination, whereas RSM provides excellent analysis for biomass harvesting optimization using a flocculation technique.					
35130096	9	38	theme	flocculation	1116:1127	arg1	efficiency					1129:1138	the flocculation efficiency	1112:1138	the flocculation efficiency of Botryococcus sp	1112:1157	Both coagulant dosage and pH significantly (p < 0.05) affect the flocculation efficiency of Botryococcus sp.					
35130096	7	39	theme	phycoremediation	916:931	arg1	12 days					905:911	12 days	905:911	12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively	905:972	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	12	40	theme	Botryococcus	1432:1443	arg1	sp					1445:1446	Botryococcus sp	1432:1446	Botryococcus sp	1432:1446	In general, Botryococcus sp.					
35130096	2	41	theme	sustainable	210:220	arg1	method					222:227	a sustainable method	208:227	a sustainable method for eco-friendly wastewater phycoremediation and valuable biomass production	208:304	Microalgae cultivation is well known as a sustainable method for eco-friendly wastewater phycoremediation and valuable biomass production.					
35130096	2	41	theme	sustainable	210:220	arg1	cultivation					179:189	Microalgae cultivation	168:189	Microalgae cultivation	168:189	Microalgae cultivation is well known as a sustainable method for eco-friendly wastewater phycoremediation and valuable biomass production.					
35130096	5	42	theme	surface	520:526	arg1	RSM					541:543	RSM	541:543	RSM	541:543	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	5	42	theme	surface	520:526	arg1	methodology					528:538	response surface methodology	511:538	response surface methodology (RSM) via face-centered central composite design (FCCCD)	511:595	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	9	43	theme	sp	1156:1157	arg1	efficiency					1129:1138	the flocculation efficiency	1112:1138	the flocculation efficiency of Botryococcus sp	1112:1157	Both coagulant dosage and pH significantly (p < 0.05) affect the flocculation efficiency of Botryococcus sp.					
35130096	7	44	theme	1.83 mg	774:780	arg1	day-1with					782:790	1.83 mg day-1with	774:790	1.83 mg day-1with	774:790	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	11	45	theme	optimum	1353:1359	arg1	dosage					1370:1375	optimum chitosan dosage	1353:1375	optimum chitosan dosage	1353:1375	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	5	46	theme	pH	665:666	arg1	sensitivity					668:678	pH sensitivity	665:678	pH sensitivity	665:678	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	13	47	theme	harvesting	1556:1565	arg1	optimization					1567:1578	biomass harvesting optimization	1548:1578	biomass harvesting optimization using a flocculation technique	1548:1609	shows a great removal efficiency of FPW contamination, whereas RSM provides excellent analysis for biomass harvesting optimization using a flocculation technique.					
35130096	5	48	theme	face-centered	550:562	arg1	FCCCD					590:594	FCCCD	590:594	FCCCD	590:594	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	5	48	theme	face-centered	550:562	arg1	design					582:587	face-centered central composite design	550:587	face-centered central composite design (FCCCD)	550:595	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	11	49	theme	coagulant	1288:1296	arg1	alum					1283:1286	alum coagulant	1283:1296	alum coagulant	1283:1296	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	5	50	theme	composite	572:580	arg1	FCCCD					590:594	FCCCD	590:594	FCCCD	590:594	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	5	50	theme	composite	572:580	arg1	design					582:587	face-centered central composite design	550:587	face-centered central composite design (FCCCD)	550:595	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	8	51	theme	polynomial	990:999	arg1	function					1001:1008	A second-order polynomial function	975:1008	A second-order polynomial function	975:1008	A second-order polynomial function fits well with the experimental results.					
35130096	13	52	theme	excellent	1525:1533	arg1	analysis					1535:1542	excellent analysis	1525:1542	excellent analysis	1525:1542	shows a great removal efficiency of FPW contamination, whereas RSM provides excellent analysis for biomass harvesting optimization using a flocculation technique.					
35130096	11	53	theme	%	1309:1309	arg1	efficiency					1324:1333	94.9% flocculation efficiency	1305:1333	94.9% flocculation efficiency	1305:1333	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	2	54	theme	valuable	278:285	arg1	production					295:304	valuable biomass production	278:304	valuable biomass production	278:304	Microalgae cultivation is well known as a sustainable method for eco-friendly wastewater phycoremediation and valuable biomass production.					
35130096	7	55	theme	carbon	859:864	arg1	removal					804:810	the highest removal	792:810	the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP)	792:897	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	3	56	from	wastewater	423:432	arg1	feasibility					335:345	the feasibility	331:345	the feasibility	331:345	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	3	56	from	wastewater	423:432	arg1	removal					359:365	kinetic removal	351:365	kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp	351:460	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	4	57	theme	enclosed	469:476	arg1	photobioreactor					478:492	an enclosed photobioreactor	466:492	an enclosed photobioreactor	466:492	in an enclosed photobioreactor.					
35130096	6	58	theme	maximum	712:718	arg1	rate					727:730	The maximum growth rate	708:730	The maximum growth rate of Botryococcus sp	708:749	The maximum growth rate of Botryococcus sp.					
35130096	13	59	theme	great	1457:1461	arg1	efficiency					1471:1480	a great removal efficiency	1455:1480	a great removal efficiency of FPW contamination	1455:1501	shows a great removal efficiency of FPW contamination, whereas RSM provides excellent analysis for biomass harvesting optimization using a flocculation technique.					
35130096	1	60	theme	enclosed	142:149	arg1	photobioreactor					151:165	an enclosed photobioreactor	139:165	an enclosed photobioreactor	139:165	cultivated in food processing wastewater using an enclosed photobioreactor.					
35130096	3	61	theme	kinetic	351:357	arg1	removal					359:365	kinetic removal	351:365	kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp	351:460	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	0	62	theme	sp	88:89	arg1	optimization					48:59	optimization	48:59	optimization of microalgae Botryococcus sp	48:89	Outdoor phycoremediation and biomass harvesting optimization of microalgae Botryococcus sp.					
35130096	7	63	dep	cultivated	752:761	arg1	day-1with					782:790	1.83 mg day-1with	774:790	1.83 mg day-1with	774:790	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	3	64	theme	nutrients	392:400	arg1	feasibility					335:345	the feasibility	331:345	the feasibility	331:345	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	3	64	theme	nutrients	392:400	arg1	removal					359:365	kinetic removal	351:365	kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp	351:460	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	7	65	theme	%	958:958	arg1	phycoremediation					916:931	phycoremediation	916:931	phycoremediation of 96.1%, 87.2%, and 35.4%, respectively	916:972	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	3	66	theme	compounds	378:386	arg1	feasibility					335:345	the feasibility	331:345	the feasibility	331:345	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	3	66	theme	compounds	378:386	arg1	removal					359:365	kinetic removal	351:365	kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp	351:460	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	5	67	theme	chitosan	645:652	arg1	effects					625:631	the effects	621:631	the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency	621:705	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	11	68	theme	30 mg	1387:1391	arg1	L-1and					1393:1398	30 mg L-1and 5.54	1387:1403	30 mg L-1and 5.54	1387:1403	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	12	69	dep	sp	1445:1446	arg1	general					1423:1429	general	1423:1429	general	1423:1429	In general, Botryococcus sp.					
35130096	8	70	theme	experimental	1029:1040	arg1	results					1042:1048	the experimental results	1025:1048	the experimental results	1025:1048	A second-order polynomial function fits well with the experimental results.					
35130096	7	71	theme	%	947:947	arg1	phycoremediation					916:931	phycoremediation	916:931	phycoremediation of 96.1%, 87.2%, and 35.4%, respectively	916:972	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	3	72	theme	food	407:410	arg1	FPW					435:437	FPW	435:437	FPW	435:437	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	3	72	theme	food	407:410	arg1	wastewater					423:432	food processing wastewater	407:432	food processing wastewater (FPW) using Botryococcus sp	407:460	This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp.					
35130096	11	73	theme	L-1and	1266:1271	arg1	pH					1273:1274	166 mg L-1and pH 12	1259:1277	166 mg L-1and pH 12	1259:1277	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	6	74	theme	Botryococcus	735:746	arg1	sp					748:749	Botryococcus sp	735:749	Botryococcus sp	735:749	The maximum growth rate of Botryococcus sp.					
35130096	7	75	theme	total	845:849	arg1	TOC					867:869	TOC	867:869	TOC	867:869	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	7	75	theme	total	845:849	arg1	carbon					859:864	total organic carbon	845:864	total organic carbon (TOC)	845:870	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	5	76	theme	alum	636:639	arg1	effects					625:631	the effects	621:631	the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency	621:705	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	1	77	theme	processing	111:120	arg1	wastewater					122:131	food processing wastewater	106:131	food processing wastewater using an enclosed photobioreactor	106:165	cultivated in food processing wastewater using an enclosed photobioreactor.					
35130096	13	78	theme	FPW	1485:1487	arg1	contamination					1489:1501	FPW contamination	1485:1501	FPW contamination	1485:1501	shows a great removal efficiency of FPW contamination, whereas RSM provides excellent analysis for biomass harvesting optimization using a flocculation technique.					
35130096	11	79	theme	L-1and	1393:1398	arg1	pH					1381:1382	pH	1381:1382	pH	1381:1382	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	11	79	theme	L-1and	1393:1398	arg1	dosage					1370:1375	optimum chitosan dosage	1353:1375	optimum chitosan dosage	1353:1375	The highest flocculation efficiency (92.4%) was obtained at a dosage of 166 mg L-1and pH 12 for alum coagulant, while 94.9% flocculation efficiency was achieved with optimum chitosan dosage and pH of 30 mg L-1and 5.54, respectively.					
35130096	0	80	theme	microalgae	64:73	arg1	sp					88:89	microalgae Botryococcus sp	64:89	microalgae Botryococcus sp	64:89	Outdoor phycoremediation and biomass harvesting optimization of microalgae Botryococcus sp.					
35130096	2	81	theme	eco-friendly	233:244	arg1	phycoremediation					257:272	eco-friendly wastewater phycoremediation	233:272	eco-friendly wastewater phycoremediation	233:272	Microalgae cultivation is well known as a sustainable method for eco-friendly wastewater phycoremediation and valuable biomass production.					
35130096	7	82	theme	oxygen	824:829	arg1	demand					831:836	chemical oxygen demand	815:836	chemical oxygen demand (COD)	815:842	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	7	82	theme	oxygen	824:829	arg1	COD					839:841	COD	839:841	COD	839:841	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
35130096	5	83	theme	response	511:518	arg1	RSM					541:543	RSM	541:543	RSM	541:543	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	5	83	theme	response	511:518	arg1	methodology					528:538	response surface methodology	511:538	response surface methodology (RSM) via face-centered central composite design (FCCCD)	511:595	Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency.					
35130096	9	84	theme	Botryococcus	1143:1154	arg1	sp					1156:1157	Botryococcus sp	1143:1157	Botryococcus sp	1143:1157	Both coagulant dosage and pH significantly (p < 0.05) affect the flocculation efficiency of Botryococcus sp.					
35130096	7	85	theme	highest	796:802	arg1	removal					804:810	the highest removal	792:810	the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP)	792:897	cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively.					
37390719	1	0	theme	C	291:291	arg1	virus					293:297	anti-hepatitis C virus	276:297	the anti-hepatitis C virus (HCV) drug sofosbuvir	272:319	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	1	0	theme	C	291:291	arg1	HCV					300:302	HCV	300:302	HCV	300:302	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	4	1	dep	rate	693:696	arg1	Kb					698:699	Kb	698:699	the binding constant rate Kb	672:699	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	1	2	theme	virus	293:297	arg1	sofosbuvir					310:319	the anti-hepatitis C virus (HCV) drug sofosbuvir	272:319	the anti-hepatitis C virus (HCV) drug sofosbuvir	272:319	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	2	3	theme	different	521:529	arg1	techniques					531:540	several different techniques	513:540	several different techniques	513:540	The characterization procedure works to verify the creation of nanocomposite (NCP) using several different techniques.					
37390719	5	4	theme	release	789:795	arg1	rate					797:800	the release rate	785:800	the release rate	785:800	At pH 7.4, the release rate was 80.6% after two hours and 92% after 48 h, whereas at pH 6.8, it was 29% after two hours and 94% after 48 h.					
37390719	5	4	theme	release	789:795	arg1	%					810:810	80.6%	806:810	80.6% after two hours	806:826	At pH 7.4, the release rate was 80.6% after two hours and 92% after 48 h, whereas at pH 6.8, it was 29% after two hours and 94% after 48 h.					
37390719	7	5	theme	fast	1013:1016	arg1	screening					1018:1026	fast screening	1013:1026	fast screening	1013:1026	The SRB technique for fast screening is used for the cytotoxicity test, where the investigated composites show a safety status and high viability against the examined cell line.					
37390719	1	6	theme	novel	219:223	arg1	Pyc.SOF					250:256	Cs@Pyc.SOF	247:256	Cs@Pyc.SOF	247:256	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	1	6	theme	novel	219:223	arg1	nanocomposite					232:244	a novel hybrid nanocomposite	217:244	a novel hybrid nanocomposite (Cs@Pyc.SOF)	217:257	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	8	7	theme	mouse	1261:1265	arg1	cells					1280:1284	mouse normal liver cells	1261:1284	mouse normal liver cells (BNL)	1261:1290	The cytotoxicity assay of the SOF hybrid materials has been identified with cell lines like mouse normal liver cells (BNL).					
37390719	8	7	theme	mouse	1261:1265	arg1	BNL					1287:1289	BNL	1287:1289	BNL	1287:1289	The cytotoxicity assay of the SOF hybrid materials has been identified with cell lines like mouse normal liver cells (BNL).					
37390719	2	8	theme	several	513:519	arg1	techniques					531:540	several different techniques	513:540	several different techniques	513:540	The characterization procedure works to verify the creation of nanocomposite (NCP) using several different techniques.					
37390719	1	9	theme	nano	368:371	arg1	biomolecules					373:384	nano biomolecules	368:384	nano biomolecules like chitosan nanoparticles (Cs NPs)	368:421	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	1	10	theme	hybrid	225:230	arg1	Pyc.SOF					250:256	Cs@Pyc.SOF	247:256	Cs@Pyc.SOF	247:256	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	1	10	theme	hybrid	225:230	arg1	nanocomposite					232:244	a novel hybrid nanocomposite	217:244	a novel hybrid nanocomposite (Cs@Pyc.SOF)	217:257	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	9	11	theme	clinical	1396:1403	arg1	studies					1405:1411	clinical studies	1396:1411	clinical studies	1396:1411	So, Cs@Pyc.SOF was recommended as a substitute medication for the therapy of HCV, but the results need clinical studies.					
37390719	4	12	theme	constant	684:691	arg1	rate					693:696	the binding constant rate	672:696	the binding constant rate Kb	672:699	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	4	12	theme	constant	684:691	arg1	7.35 ± 0.95 min-1					724:740	7.35 ± 0.95 min-1	724:740	7.35 ± 0.95 min-1	724:740	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	5	13	from	pH	859:860	arg1	%					876:876	29%	874:876	29% after two hours	874:892	At pH 7.4, the release rate was 80.6% after two hours and 92% after 48 h, whereas at pH 6.8, it was 29% after two hours and 94% after 48 h.					
37390719	5	13	from	pH	859:860	arg1	%					900:900	94%	898:900	94% after 48 h	898:911	At pH 7.4, the release rate was 80.6% after two hours and 92% after 48 h, whereas at pH 6.8, it was 29% after two hours and 94% after 48 h.					
37390719	8	14	theme	liver	1274:1278	arg1	cells					1280:1284	mouse normal liver cells	1261:1284	mouse normal liver cells (BNL)	1261:1290	The cytotoxicity assay of the SOF hybrid materials has been identified with cell lines like mouse normal liver cells (BNL).					
37390719	8	14	theme	liver	1274:1278	arg1	BNL					1287:1289	BNL	1287:1289	BNL	1287:1289	The cytotoxicity assay of the SOF hybrid materials has been identified with cell lines like mouse normal liver cells (BNL).					
37390719	4	15	theme	%	752:752	arg1	efficiency					762:771	an 83% loading efficiency	747:771	an 83% loading efficiency	747:771	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	7	16	theme	safety	1104:1109	arg1	status					1111:1116	a safety status	1102:1116	a safety status	1102:1116	The SRB technique for fast screening is used for the cytotoxicity test, where the investigated composites show a safety status and high viability against the examined cell line.					
37390719	0	17	theme	delivery	83:90	arg1	system					92:97	a delivery system	81:97	a delivery system of the sofosbuvir drug	81:120	Spectroscopic study to verify the anti-hepatitis C virus (HCV) treatment through a delivery system of the sofosbuvir drug on chitosan and pycnogenol nanoparticles surface.					
37390719	1	18	theme	drug	305:308	arg1	sofosbuvir					310:319	the anti-hepatitis C virus (HCV) drug sofosbuvir	272:319	the anti-hepatitis C virus (HCV) drug sofosbuvir	272:319	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	4	19	theme	83	750:751	arg1	%					752:752	%	752:752	%	752:752	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	1	20	theme	chitosan	391:398	arg1	nanoparticles					400:412	chitosan nanoparticles	391:412	chitosan nanoparticles (Cs NPs)	391:421	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	1	20	theme	chitosan	391:398	arg1	NPs					418:420	Cs NPs	415:420	Cs NPs	415:420	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	8	21	theme	cytotoxicity	1173:1184	arg1	assay					1186:1190	The cytotoxicity assay	1169:1190	The cytotoxicity assay of the SOF hybrid materials	1169:1218	The cytotoxicity assay of the SOF hybrid materials has been identified with cell lines like mouse normal liver cells (BNL).					
37390719	9	22	theme	HCV	1370:1372	arg1	therapy					1359:1365	the therapy	1355:1365	the therapy of HCV	1355:1372	So, Cs@Pyc.SOF was recommended as a substitute medication for the therapy of HCV, but the results need clinical studies.					
37390719	9	23	theme	Cs	1297:1298	arg1	Pyc.SOF					1300:1306	Cs@Pyc.SOF	1297:1306	Cs@Pyc.SOF	1297:1306	So, Cs@Pyc.SOF was recommended as a substitute medication for the therapy of HCV, but the results need clinical studies.					
37390719	7	24	theme	investigated	1073:1084	arg1	composites					1086:1095	the investigated composites	1069:1095	the investigated composites	1069:1095	The SRB technique for fast screening is used for the cytotoxicity test, where the investigated composites show a safety status and high viability against the examined cell line.					
37390719	0	25	theme	Spectroscopic	0:12	arg1	study					14:18	Spectroscopic study	0:18	Spectroscopic study to verify the anti-hepatitis C virus (HCV) treatment through a delivery system of the sofosbuvir drug on chitosan and pycnogenol	0:147	Spectroscopic study to verify the anti-hepatitis C virus (HCV) treatment through a delivery system of the sofosbuvir drug on chitosan and pycnogenol nanoparticles surface.					
37390719	4	26	theme	loading	754:760	arg1	efficiency					762:771	an 83% loading efficiency	747:771	an 83% loading efficiency	747:771	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	9	27	theme	@	1299:1299	arg1	Pyc.SOF					1300:1306	Cs@Pyc.SOF	1297:1306	Cs@Pyc.SOF	1297:1306	So, Cs@Pyc.SOF was recommended as a substitute medication for the therapy of HCV, but the results need clinical studies.					
37390719	6	28	from	rate	944:947	arg1	water					952:956	water	952:956	water	952:956	After 2 and 48 h, the release rate in water was 38% and 77%, respectively.					
37390719	8	29	theme	SOF	1199:1201	arg1	materials					1210:1218	the SOF hybrid materials	1195:1218	the SOF hybrid materials	1195:1218	The cytotoxicity assay of the SOF hybrid materials has been identified with cell lines like mouse normal liver cells (BNL).					
37390719	8	30	theme	cell	1245:1248	arg1	lines					1250:1254	cell lines	1245:1254	cell lines like mouse normal liver cells (BNL)	1245:1290	The cytotoxicity assay of the SOF hybrid materials has been identified with cell lines like mouse normal liver cells (BNL).					
37390719	1	31	theme	Cs	247:248	arg1	Pyc.SOF					250:256	Cs@Pyc.SOF	247:256	Cs@Pyc.SOF	247:256	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	1	31	theme	Cs	247:248	arg1	nanocomposite					232:244	a novel hybrid nanocomposite	217:244	a novel hybrid nanocomposite (Cs@Pyc.SOF)	217:257	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	8	32	theme	materials	1210:1218	arg1	assay					1186:1190	The cytotoxicity assay	1169:1190	The cytotoxicity assay of the SOF hybrid materials	1169:1218	The cytotoxicity assay of the SOF hybrid materials has been identified with cell lines like mouse normal liver cells (BNL).					
37390719	1	33	theme	Cs	415:416	arg1	nanoparticles					400:412	chitosan nanoparticles	391:412	chitosan nanoparticles (Cs NPs)	391:421	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	1	33	theme	Cs	415:416	arg1	NPs					418:420	Cs NPs	415:420	Cs NPs	415:420	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	4	34	theme	binding	676:682	arg1	rate					693:696	the binding constant rate	672:696	the binding constant rate Kb	672:699	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	4	34	theme	binding	676:682	arg1	7.35 ± 0.95 min-1					724:740	7.35 ± 0.95 min-1	724:740	7.35 ± 0.95 min-1	724:740	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	1	35	theme	@	249:249	arg1	Pyc.SOF					250:256	Cs@Pyc.SOF	247:256	Cs@Pyc.SOF	247:256	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	1	35	theme	@	249:249	arg1	nanocomposite					232:244	a novel hybrid nanocomposite	217:244	a novel hybrid nanocomposite (Cs@Pyc.SOF)	217:257	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	8	36	theme	hybrid	1203:1208	arg1	materials					1210:1218	the SOF hybrid materials	1195:1218	the SOF hybrid materials	1195:1218	The cytotoxicity assay of the SOF hybrid materials has been identified with cell lines like mouse normal liver cells (BNL).					
37390719	6	37	theme	release	936:942	arg1	%					964:964	38%	962:964	38%	962:964	After 2 and 48 h, the release rate in water was 38% and 77%, respectively.					
37390719	6	37	theme	release	936:942	arg1	rate					944:947	the release rate	932:947	the release rate in water	932:956	After 2 and 48 h, the release rate in water was 38% and 77%, respectively.					
37390719	0	38	theme	anti-hepatitis	34:47	arg1	HCV					58:60	HCV	58:60	HCV	58:60	Spectroscopic study to verify the anti-hepatitis C virus (HCV) treatment through a delivery system of the sofosbuvir drug on chitosan and pycnogenol nanoparticles surface.					
37390719	0	38	theme	anti-hepatitis	34:47	arg1	virus					51:55	anti-hepatitis C virus	34:55	the anti-hepatitis C virus (HCV) treatment	30:71	Spectroscopic study to verify the anti-hepatitis C virus (HCV) treatment through a delivery system of the sofosbuvir drug on chitosan and pycnogenol nanoparticles surface.					
37390719	7	39	theme	cytotoxicity	1044:1055	arg1	test					1057:1060	the cytotoxicity test	1040:1060	the cytotoxicity test	1040:1060	The SRB technique for fast screening is used for the cytotoxicity test, where the investigated composites show a safety status and high viability against the examined cell line.					
37390719	0	40	theme	drug	117:120	arg1	system					92:97	a delivery system	81:97	a delivery system of the sofosbuvir drug	81:120	Spectroscopic study to verify the anti-hepatitis C virus (HCV) treatment through a delivery system of the sofosbuvir drug on chitosan and pycnogenol nanoparticles surface.					
37390719	3	41	theme	SOF	582:584	arg1	efficiency					594:603	SOF loading efficiency	582:603	SOF loading efficiency	582:603	UV-Vis spectroscopy is used to measure SOF loading efficiency.					
37390719	7	42	theme	cell	1158:1161	arg1	line					1163:1166	the examined cell line	1145:1166	the examined cell line	1145:1166	The SRB technique for fast screening is used for the cytotoxicity test, where the investigated composites show a safety status and high viability against the examined cell line.					
37390719	0	43	theme	sofosbuvir	106:115	arg1	drug					117:120	the sofosbuvir drug	102:120	the sofosbuvir drug	102:120	Spectroscopic study to verify the anti-hepatitis C virus (HCV) treatment through a delivery system of the sofosbuvir drug on chitosan and pycnogenol nanoparticles surface.					
37390719	1	44	theme	nano	330:333	arg1	Pyc					359:361	Pyc	359:361	Pyc	359:361	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	1	44	theme	nano	330:333	arg1	pycnogenol					347:356	the nano antioxidant pycnogenol	326:356	the nano antioxidant pycnogenol (Pyc)	326:362	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	7	45	used	used	1031:1034	arg2	technique					999:1007	The SRB technique	991:1007	The SRB technique for fast screening	991:1026	The SRB technique for fast screening is used for the cytotoxicity test, where the investigated composites show a safety status and high viability against the examined cell line.					
37390719	1	46	theme	current	190:196	arg1	study					198:202	the current study	186:202	the current study	186:202	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	0	47	theme	virus	51:55	arg1	treatment					63:71	the anti-hepatitis C virus (HCV) treatment	30:71	the anti-hepatitis C virus (HCV) treatment	30:71	Spectroscopic study to verify the anti-hepatitis C virus (HCV) treatment through a delivery system of the sofosbuvir drug on chitosan and pycnogenol nanoparticles surface.					
37390719	1	48	theme	antioxidant	335:345	arg1	Pyc					359:361	Pyc	359:361	Pyc	359:361	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	1	48	theme	antioxidant	335:345	arg1	pycnogenol					347:356	the nano antioxidant pycnogenol	326:356	the nano antioxidant pycnogenol (Pyc)	326:362	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	4	49	theme	drug	644:647	arg1	concentrations					618:631	The various concentrations	606:631	The various concentrations of the SOF drug	606:647	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	0	50	theme	C	49:49	arg1	HCV					58:60	HCV	58:60	HCV	58:60	Spectroscopic study to verify the anti-hepatitis C virus (HCV) treatment through a delivery system of the sofosbuvir drug on chitosan and pycnogenol nanoparticles surface.					
37390719	0	50	theme	C	49:49	arg1	virus					51:55	anti-hepatitis C virus	34:55	the anti-hepatitis C virus (HCV) treatment	30:71	Spectroscopic study to verify the anti-hepatitis C virus (HCV) treatment through a delivery system of the sofosbuvir drug on chitosan and pycnogenol nanoparticles surface.					
37390719	3	51	used	used	566:569	arg2	spectroscopy					550:561	UV-Vis spectroscopy	543:561	UV-Vis spectroscopy	543:561	UV-Vis spectroscopy is used to measure SOF loading efficiency.					
37390719	4	52	theme	SOF	640:642	arg1	drug					644:647	the SOF drug	636:647	the SOF drug	636:647	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	4	53	used	used	654:657	arg2	concentrations					618:631	The various concentrations	606:631	The various concentrations of the SOF drug	606:647	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	2	54	theme	characterization	428:443	arg1	procedure					445:453	The characterization procedure	424:453	The characterization procedure	424:453	The characterization procedure works to verify the creation of nanocomposite (NCP) using several different techniques.					
37390719	1	55	theme	study	198:202	arg1	target					176:181	The target	172:181	The target of the current study	172:202	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	2	56	theme	nanocomposite	487:499	arg1	creation					475:482	the creation	471:482	the creation of nanocomposite (NCP) using several different techniques	471:540	The characterization procedure works to verify the creation of nanocomposite (NCP) using several different techniques.					
37390719	3	57	theme	loading	586:592	arg1	efficiency					594:603	SOF loading efficiency	582:603	SOF loading efficiency	582:603	UV-Vis spectroscopy is used to measure SOF loading efficiency.					
37390719	7	58	theme	high	1122:1125	arg1	viability					1127:1135	high viability	1122:1135	high viability	1122:1135	The SRB technique for fast screening is used for the cytotoxicity test, where the investigated composites show a safety status and high viability against the examined cell line.					
37390719	5	59	from	pH	777:778	arg1	rate					797:800	the release rate	785:800	the release rate	785:800	At pH 7.4, the release rate was 80.6% after two hours and 92% after 48 h, whereas at pH 6.8, it was 29% after two hours and 94% after 48 h.					
37390719	5	59	from	pH	777:778	arg1	%					810:810	80.6%	806:810	80.6% after two hours	806:826	At pH 7.4, the release rate was 80.6% after two hours and 92% after 48 h, whereas at pH 6.8, it was 29% after two hours and 94% after 48 h.					
37390719	5	59	from	pH	777:778	arg1	%					834:834	92%	832:834	92%	832:834	At pH 7.4, the release rate was 80.6% after two hours and 92% after 48 h, whereas at pH 6.8, it was 29% after two hours and 94% after 48 h.					
37390719	7	60	theme	SRB	995:997	arg1	technique					999:1007	The SRB technique	991:1007	The SRB technique for fast screening	991:1026	The SRB technique for fast screening is used for the cytotoxicity test, where the investigated composites show a safety status and high viability against the examined cell line.					
37390719	0	61	dep	study	14:18	arg1	verify					23:28	verify	23:28	to verify the anti-hepatitis C virus (HCV) treatment through a delivery system of the sofosbuvir drug on chitosan and pycnogenol	20:147	Spectroscopic study to verify the anti-hepatitis C virus (HCV) treatment through a delivery system of the sofosbuvir drug on chitosan and pycnogenol nanoparticles surface.					
37390719	4	62	with	7.35 ± 0.95 min-1	724:740	arg1	efficiency					762:771	an 83% loading efficiency	747:771	an 83% loading efficiency	747:771	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	1	63	theme	anti-hepatitis	276:289	arg1	virus					293:297	anti-hepatitis C virus	276:297	the anti-hepatitis C virus (HCV) drug sofosbuvir	272:319	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	1	63	theme	anti-hepatitis	276:289	arg1	HCV					300:302	HCV	300:302	HCV	300:302	The target of the current study is to create a novel hybrid nanocomposite (Cs@Pyc.SOF) by combining the anti-hepatitis C virus (HCV) drug sofosbuvir with the nano antioxidant pycnogenol (Pyc) and nano biomolecules like chitosan nanoparticles (Cs NPs).					
37390719	3	64	theme	UV-Vis	543:548	arg1	spectroscopy					550:561	UV-Vis spectroscopy	543:561	UV-Vis spectroscopy	543:561	UV-Vis spectroscopy is used to measure SOF loading efficiency.					
37390719	4	65	theme	various	610:616	arg1	concentrations					618:631	The various concentrations	606:631	The various concentrations of the SOF drug	606:647	The various concentrations of the SOF drug were used to determine the binding constant rate Kb, which was found to be 7.35 ± 0.95 min-1 with an 83% loading efficiency.					
37390719	8	66	theme	normal	1267:1272	arg1	cells					1280:1284	mouse normal liver cells	1261:1284	mouse normal liver cells (BNL)	1261:1290	The cytotoxicity assay of the SOF hybrid materials has been identified with cell lines like mouse normal liver cells (BNL).					
37390719	8	66	theme	normal	1267:1272	arg1	BNL					1287:1289	BNL	1287:1289	BNL	1287:1289	The cytotoxicity assay of the SOF hybrid materials has been identified with cell lines like mouse normal liver cells (BNL).					
37390719	7	67	theme	examined	1149:1156	arg1	line					1163:1166	the examined cell line	1145:1166	the examined cell line	1145:1166	The SRB technique for fast screening is used for the cytotoxicity test, where the investigated composites show a safety status and high viability against the examined cell line.					
35787878	0	0	theme	sustainable	76:86	arg1	electrocatalyst					88:102	A sustainable electrocatalyst	74:102	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.	0:147	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.					
35787878	8	1	theme	11.64 μA μM-1	1337:1349	arg1	cm-2					1351:1354	11.64 μA μM-1 cm-2	1337:1354	11.64 μA μM-1 cm-2	1337:1354	The sensor also exhibited a high sensitivity of 11.64 μA μM-1 cm-2.					
35787878	1	2	theme	ill	153:155	arg1	rifampicin					215:224	rifampicin	215:224	rifampicin	215:224	The ill effects of prolonged use of rifamycin antibiotics such as rifampicin accentuates its need for detection in the environment as well as in biological fluids.					
35787878	1	2	theme	ill	153:155	arg1	effects					157:163	The ill effects	149:163	The ill effects of prolonged use of rifamycin antibiotics such as rifampicin	149:224	The ill effects of prolonged use of rifamycin antibiotics such as rifampicin accentuates its need for detection in the environment as well as in biological fluids.					
35787878	7	3	dep	374.5 μM	1279:1286	arg1	to					1276:1277	to	1276:1277	to	1276:1277	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	8	4	theme	cm-2	1351:1354	arg1	sensitivity					1322:1332	a high sensitivity	1315:1332	a high sensitivity of 11.64 μA μM-1 cm-2	1315:1354	The sensor also exhibited a high sensitivity of 11.64 μA μM-1 cm-2.					
35787878	0	5	from	orchestration	10:22	arg1	rGO					69:71	β-cd functionalized rGO	49:71	β-cd functionalized rGO	49:71	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.					
35787878	4	6	theme	composite	650:658	arg1	formation					630:638	The formation	626:638	The formation of hybrid composite	626:658	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	5	7	theme	microspheres	915:926	arg1	formation					902:910	The formation	898:910	The formation of microspheres	898:926	The formation of microspheres were observed with hexagonal crystal system and P63/mmc space group.					
35787878	4	8	theme	EDX	884:886	arg1	analysis					888:895	EDX analysis	884:895	EDX analysis	884:895	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	2	9	from	Antibiotics	313:323	arg1	water					328:332	water	328:332	water	328:332	Antibiotics in water and soil are long-lasting, bio-accumulative, and hazardous to aquatic species as well as human health.					
35787878	2	9	from	Antibiotics	313:323	arg1	soil					338:341	soil	338:341	soil	338:341	Antibiotics in water and soil are long-lasting, bio-accumulative, and hazardous to aquatic species as well as human health.					
35787878	4	10	theme	Raman	713:717	arg1	analysis					697:704	X-ray diffraction analysis	679:704	X-ray diffraction analysis (XRD)	679:710	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	10	theme	Raman	713:717	arg1	spectroscopy					719:730	Raman spectroscopy	713:730	Raman spectroscopy	713:730	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	9	11	theme	fish	1504:1507	arg1	samples					1509:1515	industrial water, and fish samples	1482:1515	samples	1509:1515	Additionally, the sensor showed appreciable recovery range when monitored in real-samples such as human serum and urine, and industrial water, and fish samples.					
35787878	6	12	theme	used	1111:1114	arg1	RIF					1140:1142	RIF	1140:1142	RIF	1140:1142	Furthermore, the composite was employed to fabricate an efficient electrochemical sensor for detecting the widely used antibiotic, rifampicin (RIF).					
35787878	6	12	theme	used	1111:1114	arg1	rifampicin					1128:1137	rifampicin	1128:1137	rifampicin (RIF)	1128:1143	Furthermore, the composite was employed to fabricate an efficient electrochemical sensor for detecting the widely used antibiotic, rifampicin (RIF).					
35787878	0	13	theme	Strategic	0:8	arg1	orchestration					10:22	Strategic orchestration	0:22	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.	0:147	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.					
35787878	6	14	theme	efficient	1053:1061	arg1	sensor					1079:1084	an efficient electrochemical sensor	1050:1084	an efficient electrochemical sensor for detecting the widely used antibiotic, rifampicin (RIF)	1050:1143	Furthermore, the composite was employed to fabricate an efficient electrochemical sensor for detecting the widely used antibiotic, rifampicin (RIF).					
35787878	4	15	theme	photoelectron	788:800	arg1	spectroscopy					802:813	X-ray photoelectron spectroscopy	782:813	X-ray photoelectron spectroscopy (XPS)	782:819	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	15	theme	photoelectron	788:800	arg1	XPS					816:818	XPS	816:818	XPS	816:818	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	7	16	theme	28 nM	1233:1237	arg1	LOD					1225:1227	LOD	1225:1227	LOD	1225:1227	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	7	16	theme	28 nM	1233:1237	arg1	detection					1214:1222	detection	1214:1222	detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM	1214:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	4	17	theme	X-ray	782:786	arg1	spectroscopy					802:813	X-ray photoelectron spectroscopy	782:813	X-ray photoelectron spectroscopy (XPS)	782:819	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	17	theme	X-ray	782:786	arg1	XPS					816:818	XPS	816:818	XPS	816:818	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	3	18	theme	Molybdenum	504:513	arg1	diselenide					515:524	Molybdenum diselenide	504:524	Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer	504:623	To address this issue, a sensing platform has been developed using Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer.					
35787878	3	18	theme	Molybdenum	504:513	arg1	MoSe2					527:531	MoSe2	527:531	MoSe2	527:531	To address this issue, a sensing platform has been developed using Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer.					
35787878	1	19	theme	prolonged	168:176	arg1	use					178:180	prolonged use	168:180	prolonged use of rifamycin antibiotics	168:205	The ill effects of prolonged use of rifamycin antibiotics such as rifampicin accentuates its need for detection in the environment as well as in biological fluids.					
35787878	7	20	from	limit	1205:1209	arg1	range					1259:1263	a linear working range	1242:1263	a linear working range from 0.019 to 374.5 μM	1242:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	3	21	theme	embedded	534:541	arg1	diselenide					515:524	Molybdenum diselenide	504:524	Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer	504:623	To address this issue, a sensing platform has been developed using Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer.					
35787878	3	21	theme	embedded	534:541	arg1	MoSe2					527:531	MoSe2	527:531	MoSe2	527:531	To address this issue, a sensing platform has been developed using Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer.					
35787878	1	22	theme	use	178:180	arg1	rifampicin					215:224	rifampicin	215:224	rifampicin	215:224	The ill effects of prolonged use of rifamycin antibiotics such as rifampicin accentuates its need for detection in the environment as well as in biological fluids.					
35787878	1	22	theme	use	178:180	arg1	effects					157:163	The ill effects	149:163	The ill effects of prolonged use of rifamycin antibiotics such as rifampicin	149:224	The ill effects of prolonged use of rifamycin antibiotics such as rifampicin accentuates its need for detection in the environment as well as in biological fluids.					
35787878	2	23	theme	human	423:427	arg1	health					429:434	human health	423:434	long-lasting, bio-accumulative, and hazardous to aquatic species as well as human health	347:434	Antibiotics in water and soil are long-lasting, bio-accumulative, and hazardous to aquatic species as well as human health.					
35787878	7	24	from	detection	1214:1222	arg1	374.5 μM					1279:1286	374.5 μM	1279:1286	374.5 μM	1279:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	7	24	from	detection	1214:1222	arg1	range					1259:1263	a linear working range	1242:1263	a linear working range from 0.019 to 374.5 μM	1242:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	4	25	dep	fourier	733:739	arg1	transform					741:749	transform	741:749	transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis	741:895	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	0	26	theme	MoSe2	27:31	arg1	microspheres					33:44	MoSe2 microspheres	27:44	MoSe2 microspheres	27:44	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.					
35787878	7	27	theme	working	1251:1257	arg1	range					1259:1263	a linear working range	1242:1263	a linear working range from 0.019 to 374.5 μM	1242:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	5	28	theme	P63/mmc	976:982	arg1	group					990:994	P63/mmc space group	976:994	P63/mmc space group	976:994	The formation of microspheres were observed with hexagonal crystal system and P63/mmc space group.					
35787878	9	29	theme	industrial	1482:1491	arg1	water					1493:1497	industrial water, and fish samples	1482:1515	water	1493:1497	Additionally, the sensor showed appreciable recovery range when monitored in real-samples such as human serum and urine, and industrial water, and fish samples.					
35787878	7	30	from	374.5 μM	1279:1286	arg1	LOD					1225:1227	LOD	1225:1227	LOD	1225:1227	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	7	30	from	374.5 μM	1279:1286	arg1	range					1259:1263	a linear working range	1242:1263	a linear working range from 0.019 to 374.5 μM	1242:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	7	30	from	374.5 μM	1279:1286	arg1	detection					1214:1222	detection	1214:1222	detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM	1214:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	1	31	theme	rifamycin	185:193	arg1	antibiotics					195:205	rifamycin antibiotics	185:205	rifamycin antibiotics	185:205	The ill effects of prolonged use of rifamycin antibiotics such as rifampicin accentuates its need for detection in the environment as well as in biological fluids.					
35787878	0	32	theme	rifampicin	121:130	arg1	detection					108:116	detection	108:116	detection of rifampicin in real samples	108:146	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.					
35787878	3	33	theme	β-cd	611:614	arg1	polymer					617:623	β-cyclodextrin (β-cd) polymer	595:623	β-cyclodextrin (β-cd) polymer	595:623	To address this issue, a sensing platform has been developed using Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer.					
35787878	1	34	theme	antibiotics	195:205	arg1	use					178:180	prolonged use	168:180	prolonged use of rifamycin antibiotics	168:205	The ill effects of prolonged use of rifamycin antibiotics such as rifampicin accentuates its need for detection in the environment as well as in biological fluids.					
35787878	7	35	theme	detection	1214:1222	arg1	limit					1205:1209	a limit	1203:1209	a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM	1203:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	7	36	from	range	1259:1263	arg1	limit					1205:1209	a limit	1203:1209	a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM	1203:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	7	37	theme	sensor	1191:1196	arg1	activity					1175:1182	excellent activity	1165:1182	excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM	1165:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	5	38	theme	crystal	957:963	arg1	system					965:970	hexagonal crystal system	947:970	hexagonal crystal system	947:970	The formation of microspheres were observed with hexagonal crystal system and P63/mmc space group.					
35787878	7	39	with	activity	1175:1182	arg1	limit					1205:1209	a limit	1203:1209	a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM	1203:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	2	40	theme	aquatic	396:402	arg1	species					404:410	aquatic species	396:410	aquatic species	396:410	Antibiotics in water and soil are long-lasting, bio-accumulative, and hazardous to aquatic species as well as human health.					
35787878	0	41	theme	microspheres	33:44	arg1	orchestration					10:22	Strategic orchestration	0:22	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.	0:147	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.					
35787878	0	42	theme	real	135:138	arg1	samples					140:146	real samples	135:146	real samples	135:146	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.					
35787878	3	43	theme	β-cyclodextrin	595:608	arg1	polymer					617:623	β-cyclodextrin (β-cd) polymer	595:623	β-cyclodextrin (β-cd) polymer	595:623	To address this issue, a sensing platform has been developed using Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer.					
35787878	9	44	theme	appreciable	1389:1399	arg1	range					1410:1414	appreciable recovery range	1389:1414	appreciable recovery range	1389:1414	Additionally, the sensor showed appreciable recovery range when monitored in real-samples such as human serum and urine, and industrial water, and fish samples.					
35787878	0	45	theme	functionalized	54:67	arg1	rGO					69:71	β-cd functionalized rGO	49:71	β-cd functionalized rGO	49:71	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.					
35787878	1	46	theme	biological	294:303	arg1	fluids					305:310	biological fluids	294:310	biological fluids	294:310	The ill effects of prolonged use of rifamycin antibiotics such as rifampicin accentuates its need for detection in the environment as well as in biological fluids.					
35787878	4	47	theme	emission	832:839	arg1	FE-SEM					871:876	FE-SEM	871:876	FE-SEM	871:876	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	47	theme	emission	832:839	arg1	microscopy					859:868	field emission scanning electron microscopy	826:868	field emission scanning electron microscopy (FE-SEM)	826:877	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	3	48	theme	reduced	546:552	arg1	oxide					563:567	reduced graphene oxide	546:567	reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer	546:623	To address this issue, a sensing platform has been developed using Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer.					
35787878	3	48	theme	reduced	546:552	arg1	rGO					570:572	rGO	570:572	rGO	570:572	To address this issue, a sensing platform has been developed using Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer.					
35787878	9	49	theme	recovery	1401:1408	arg1	range					1410:1414	appreciable recovery range	1389:1414	appreciable recovery range	1389:1414	Additionally, the sensor showed appreciable recovery range when monitored in real-samples such as human serum and urine, and industrial water, and fish samples.					
35787878	0	50	theme	β-cd	49:52	arg1	rGO					69:71	β-cd functionalized rGO	49:71	β-cd functionalized rGO	49:71	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.					
35787878	4	51	theme	field	826:830	arg1	FE-SEM					871:876	FE-SEM	871:876	FE-SEM	871:876	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	51	theme	field	826:830	arg1	microscopy					859:868	field emission scanning electron microscopy	826:868	field emission scanning electron microscopy (FE-SEM)	826:877	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	5	52	theme	space	984:988	arg1	group					990:994	P63/mmc space group	976:994	P63/mmc space group	976:994	The formation of microspheres were observed with hexagonal crystal system and P63/mmc space group.					
35787878	3	53	theme	graphene	554:561	arg1	oxide					563:567	reduced graphene oxide	546:567	reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer	546:623	To address this issue, a sensing platform has been developed using Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer.					
35787878	3	53	theme	graphene	554:561	arg1	rGO					570:572	rGO	570:572	rGO	570:572	To address this issue, a sensing platform has been developed using Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer.					
35787878	3	54	theme	sensing	462:468	arg1	platform					470:477	a sensing platform	460:477	a sensing platform	460:477	To address this issue, a sensing platform has been developed using Molybdenum diselenide (MoSe2) embedded on reduced graphene oxide (rGO) functionalized with β-cyclodextrin (β-cd) polymer.					
35787878	5	55	theme	hexagonal	947:955	arg1	system					965:970	hexagonal crystal system	947:970	hexagonal crystal system	947:970	The formation of microspheres were observed with hexagonal crystal system and P63/mmc space group.					
35787878	4	56	theme	electron	850:857	arg1	FE-SEM					871:876	FE-SEM	871:876	FE-SEM	871:876	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	56	theme	electron	850:857	arg1	microscopy					859:868	field emission scanning electron microscopy	826:868	field emission scanning electron microscopy (FE-SEM)	826:877	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	0	57	from	detection	108:116	arg1	samples					140:146	real samples	135:146	real samples	135:146	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.					
35787878	4	58	theme	diffraction	685:695	arg1	XRD					707:709	XRD	707:709	XRD	707:709	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	58	theme	diffraction	685:695	arg1	analysis					697:704	X-ray diffraction analysis	679:704	X-ray diffraction analysis (XRD)	679:710	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	58	theme	diffraction	685:695	arg1	spectroscopy					719:730	Raman spectroscopy	713:730	Raman spectroscopy	713:730	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	58	theme	diffraction	685:695	arg1	fourier					733:739	fourier	733:739	fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis	733:895	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	59	theme	scanning	841:848	arg1	FE-SEM					871:876	FE-SEM	871:876	FE-SEM	871:876	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	59	theme	scanning	841:848	arg1	microscopy					859:868	field emission scanning electron microscopy	826:868	field emission scanning electron microscopy (FE-SEM)	826:877	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	9	60	theme	human	1455:1459	arg1	serum					1461:1465	human serum	1455:1465	human serum	1455:1465	Additionally, the sensor showed appreciable recovery range when monitored in real-samples such as human serum and urine, and industrial water, and fish samples.					
35787878	8	61	theme	high	1317:1320	arg1	sensitivity					1322:1332	a high sensitivity	1315:1332	a high sensitivity of 11.64 μA μM-1 cm-2	1315:1354	The sensor also exhibited a high sensitivity of 11.64 μA μM-1 cm-2.					
35787878	4	62	theme	X-ray	679:683	arg1	XRD					707:709	XRD	707:709	XRD	707:709	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	62	theme	X-ray	679:683	arg1	analysis					697:704	X-ray diffraction analysis	679:704	X-ray diffraction analysis (XRD)	679:710	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	62	theme	X-ray	679:683	arg1	spectroscopy					719:730	Raman spectroscopy	713:730	Raman spectroscopy	713:730	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	62	theme	X-ray	679:683	arg1	fourier					733:739	fourier	733:739	fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis	733:895	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	6	63	theme	electrochemical	1063:1077	arg1	sensor					1079:1084	an efficient electrochemical sensor	1050:1084	an efficient electrochemical sensor for detecting the widely used antibiotic, rifampicin (RIF)	1050:1143	Furthermore, the composite was employed to fabricate an efficient electrochemical sensor for detecting the widely used antibiotic, rifampicin (RIF).					
35787878	7	64	theme	excellent	1165:1173	arg1	activity					1175:1182	excellent activity	1165:1182	excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM	1165:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	0	65	dep	orchestration	10:22	arg1	electrocatalyst					88:102	A sustainable electrocatalyst	74:102	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.	0:147	Strategic orchestration of MoSe2 microspheres on β-cd functionalized rGO: A sustainable electrocatalyst for detection of rifampicin in real samples.					
35787878	6	66	theme	antibiotic	1116:1125	arg1	RIF					1140:1142	RIF	1140:1142	RIF	1140:1142	Furthermore, the composite was employed to fabricate an efficient electrochemical sensor for detecting the widely used antibiotic, rifampicin (RIF).					
35787878	6	66	theme	antibiotic	1116:1125	arg1	rifampicin					1128:1137	rifampicin	1128:1137	rifampicin (RIF)	1128:1143	Furthermore, the composite was employed to fabricate an efficient electrochemical sensor for detecting the widely used antibiotic, rifampicin (RIF).					
35787878	4	67	theme	hybrid	643:648	arg1	composite					650:658	hybrid composite	643:658	hybrid composite	643:658	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	7	68	theme	linear	1244:1249	arg1	range					1259:1263	a linear working range	1242:1263	a linear working range from 0.019 to 374.5 μM	1242:1286	The results reveal excellent activity of the sensor with a limit of detection (LOD) of 28 nM in a linear working range from 0.019 to 374.5 μM.					
35787878	4	69	theme	infrared	751:758	arg1	FT-IR					774:778	FT-IR	774:778	FT-IR	774:778	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
35787878	4	69	theme	infrared	751:758	arg1	spectroscopy					760:771	infrared spectroscopy	751:771	infrared spectroscopy (FT-IR)	751:779	The formation of hybrid composite was validated with X-ray diffraction analysis (XRD), Raman spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM) with EDX analysis.					
36233049	6	0	from	thermodynamics	981:994	arg1	water					1072:1076	water	1072:1076	water	1072:1076	The thermodynamics of the interaction of acetylsalicylic acid (aspirin) with β-cyclodextrin in water and DMF is fully discussed, emphasizing the two different processes that take place in water at the two different pHs.					
36233049	6	0	from	thermodynamics	981:994	arg1	DMF					1082:1084	DMF	1082:1084	DMF	1082:1084	The thermodynamics of the interaction of acetylsalicylic acid (aspirin) with β-cyclodextrin in water and DMF is fully discussed, emphasizing the two different processes that take place in water at the two different pHs.					
36233049	1	1	theme	5-acetylsalicylic	259:275	arg1	acid					277:280	5-acetylsalicylic acid	259:280	5-acetylsalicylic acid	259:280	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	1	1	theme	5-acetylsalicylic	259:275	arg1	pharmaceuticals					194:208	pharmaceuticals	194:208	pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N	194:296	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	2	2	theme	electrolytes	546:557	arg1	energies					518:525	the solution Gibbs energies	499:525	the solution Gibbs energies of the dissociated electrolytes in this solvent	499:573	Taking into account the formation of ion pairs of these pharmaceuticals in water, the solution Gibbs energies of the dissociated electrolytes in this solvent were calculated.					
36233049	4	3	theme	saturated	770:778	arg1	atmosphere					780:789	a saturated atmosphere	768:789	a saturated atmosphere of DMF	768:796	These pharmaceuticals undergo solvation when exposed to a saturated atmosphere of DMF.					
36233049	2	4	from	solvent	567:573	arg1	energies					518:525	the solution Gibbs energies	499:525	the solution Gibbs energies of the dissociated electrolytes in this solvent	499:573	Taking into account the formation of ion pairs of these pharmaceuticals in water, the solution Gibbs energies of the dissociated electrolytes in this solvent were calculated.					
36233049	2	5	theme	Gibbs	512:516	arg1	energies					518:525	the solution Gibbs energies	499:525	the solution Gibbs energies of the dissociated electrolytes in this solvent	499:573	Taking into account the formation of ion pairs of these pharmaceuticals in water, the solution Gibbs energies of the dissociated electrolytes in this solvent were calculated.					
36233049	1	6	from	N	296:296	arg1	behavior					182:189	The solution behavior	169:189	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K	169:334	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	4	7	theme	DMF	794:796	arg1	atmosphere					780:789	a saturated atmosphere	768:789	a saturated atmosphere of DMF	768:796	These pharmaceuticals undergo solvation when exposed to a saturated atmosphere of DMF.					
36233049	3	8	from	thermodynamics	611:624	arg1	water					648:652	water	648:652	water	648:652	Thus, the solution thermodynamics of these compounds in water are reported using enthalpy data obtained by calorimetry.					
36233049	2	9	theme	solution	503:510	arg1	energies					518:525	the solution Gibbs energies	499:525	the solution Gibbs energies of the dissociated electrolytes in this solvent	499:573	Taking into account the formation of ion pairs of these pharmaceuticals in water, the solution Gibbs energies of the dissociated electrolytes in this solvent were calculated.					
36233049	2	10	theme	pharmaceuticals	473:487	arg1	pairs					458:462	ion pairs	454:462	ion pairs of these pharmaceuticals	454:487	Taking into account the formation of ion pairs of these pharmaceuticals in water, the solution Gibbs energies of the dissociated electrolytes in this solvent were calculated.					
36233049	5	11	theme	enthalpy	949:956	arg1	data					958:961	only enthalpy data	944:961	only enthalpy data	944:961	As the composition of the solid is not the same as that in solution, the Gibbs energy of the solutions of these compounds could not be obtained; only enthalpy data are reported.					
36233049	1	12	theme	solution	173:180	arg1	behavior					182:189	The solution behavior	169:189	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K	169:334	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	0	13	theme	Solution	5:12	arg1	Studies					14:20	Solution Studies	5:20	Solution Studies of Pharmaceuticals (Aspirin and Related Compounds) to the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N,N-Dimethylformamide	5:166	From Solution Studies of Pharmaceuticals (Aspirin and Related Compounds) to the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N,N-Dimethylformamide.					
36233049	1	14	from	water	286:290	arg1	behavior					182:189	The solution behavior	169:189	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K	169:334	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	5	15	theme	compounds	911:919	arg1	solutions					892:900	the solutions	888:900	the solutions of these compounds	888:919	As the composition of the solid is not the same as that in solution, the Gibbs energy of the solutions of these compounds could not be obtained; only enthalpy data are reported.					
36233049	5	16	theme	solid	825:829	arg1	same					842:845	same	842:845	same	842:845	As the composition of the solid is not the same as that in solution, the Gibbs energy of the solutions of these compounds could not be obtained; only enthalpy data are reported.					
36233049	5	16	theme	solid	825:829	arg1	composition					806:816	the composition	802:816	the composition of the solid	802:829	As the composition of the solid is not the same as that in solution, the Gibbs energy of the solutions of these compounds could not be obtained; only enthalpy data are reported.					
36233049	3	17	theme	solution	602:609	arg1	thermodynamics					611:624	the solution thermodynamics	598:624	the solution thermodynamics of these compounds in water	598:652	Thus, the solution thermodynamics of these compounds in water are reported using enthalpy data obtained by calorimetry.					
36233049	1	18	theme	pharmaceuticals	194:208	arg1	behavior					182:189	The solution behavior	169:189	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K	169:334	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	7	19	with	interaction	1379:1389	arg1	guest					1400:1404	the guest	1396:1404	the guest	1396:1404	In all cases, the favorable Gibbs energies for these processes are entropically controlled, mainly resulting from the higher dehydration/desolvation that the receptor undergoes upon interaction with the guest.					
36233049	0	20	theme	Interaction	121:131	arg1	Thermodynamics					80:93	the Thermodynamics	76:93	the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N	76:146	From Solution Studies of Pharmaceuticals (Aspirin and Related Compounds) to the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N,N-Dimethylformamide.					
36233049	0	20	theme	Interaction	121:131	arg1	N-Dimethylformamide					148:166	N-Dimethylformamide	148:166	N-Dimethylformamide	148:166	From Solution Studies of Pharmaceuticals (Aspirin and Related Compounds) to the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N,N-Dimethylformamide.					
36233049	6	21	theme	acetylsalicylic	1018:1032	arg1	aspirin					1040:1046	aspirin	1040:1046	aspirin	1040:1046	The thermodynamics of the interaction of acetylsalicylic acid (aspirin) with β-cyclodextrin in water and DMF is fully discussed, emphasizing the two different processes that take place in water at the two different pHs.					
36233049	6	21	theme	acetylsalicylic	1018:1032	arg1	acid					1034:1037	acetylsalicylic acid	1018:1037	acetylsalicylic acid (aspirin)	1018:1047	The thermodynamics of the interaction of acetylsalicylic acid (aspirin) with β-cyclodextrin in water and DMF is fully discussed, emphasizing the two different processes that take place in water at the two different pHs.					
36233049	1	22	from	K	334:334	arg1	DMF					319:321	DMF	319:321	DMF	319:321	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	1	22	from	K	334:334	arg1	pharmaceuticals					194:208	pharmaceuticals	194:208	pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N	194:296	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	1	22	from	K	334:334	arg1	N-Dimethylformamide					298:316	N-Dimethylformamide	298:316	N-Dimethylformamide (DMF) at 298.15 K	298:334	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	5	23	theme	solutions	892:900	arg1	energy					878:883	the Gibbs energy	868:883	the Gibbs energy of the solutions of these compounds	868:919	As the composition of the solid is not the same as that in solution, the Gibbs energy of the solutions of these compounds could not be obtained; only enthalpy data are reported.					
36233049	2	24	theme	dissociated	534:544	arg1	electrolytes					546:557	the dissociated electrolytes	530:557	the dissociated electrolytes in this solvent	530:573	Taking into account the formation of ion pairs of these pharmaceuticals in water, the solution Gibbs energies of the dissociated electrolytes in this solvent were calculated.					
36233049	0	25	theme	Aspirin-β-Cyclodextrin	98:119	arg1	Interaction					121:131	Aspirin-β-Cyclodextrin Interaction	98:131	Aspirin-β-Cyclodextrin Interaction	98:131	From Solution Studies of Pharmaceuticals (Aspirin and Related Compounds) to the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N,N-Dimethylformamide.					
36233049	1	26	theme	acetylsalicylic	211:225	arg1	pharmaceuticals					194:208	pharmaceuticals	194:208	pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N	194:296	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	1	26	theme	acetylsalicylic	211:225	arg1	acid					227:230	acetylsalicylic acid	211:230	acetylsalicylic acid	211:230	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	0	27	theme	Pharmaceuticals	25:39	arg1	Studies					14:20	Solution Studies	5:20	Solution Studies of Pharmaceuticals (Aspirin and Related Compounds) to the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N,N-Dimethylformamide	5:166	From Solution Studies of Pharmaceuticals (Aspirin and Related Compounds) to the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N,N-Dimethylformamide.					
36233049	1	28	theme	calorimetric	390:401	arg1	measurements					403:414	calorimetric measurements	390:414	calorimetric measurements	390:414	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	6	29	theme	different	1182:1190	arg1	pHs					1192:1194	the two different pHs	1174:1194	the two different pHs	1174:1194	The thermodynamics of the interaction of acetylsalicylic acid (aspirin) with β-cyclodextrin in water and DMF is fully discussed, emphasizing the two different processes that take place in water at the two different pHs.					
36233049	6	30	theme	interaction	1003:1013	arg1	thermodynamics					981:994	The thermodynamics	977:994	The thermodynamics of the interaction of acetylsalicylic acid (aspirin) with β-cyclodextrin in water and DMF	977:1084	The thermodynamics of the interaction of acetylsalicylic acid (aspirin) with β-cyclodextrin in water and DMF is fully discussed, emphasizing the two different processes that take place in water at the two different pHs.					
36233049	7	31	theme	higher	1315:1320	arg1	dehydration/desolvation					1322:1344	the higher dehydration/desolvation	1311:1344	the higher dehydration/desolvation that the receptor undergoes upon interaction with the guest	1311:1404	In all cases, the favorable Gibbs energies for these processes are entropically controlled, mainly resulting from the higher dehydration/desolvation that the receptor undergoes upon interaction with the guest.					
36233049	7	32	theme	favorable	1215:1223	arg1	energies					1231:1238	the favorable Gibbs energies	1211:1238	the favorable Gibbs energies for these processes	1211:1258	In all cases, the favorable Gibbs energies for these processes are entropically controlled, mainly resulting from the higher dehydration/desolvation that the receptor undergoes upon interaction with the guest.					
36233049	2	33	theme	pairs	458:462	arg1	formation					441:449	the formation	437:449	the formation of ion pairs of these pharmaceuticals	437:487	Taking into account the formation of ion pairs of these pharmaceuticals in water, the solution Gibbs energies of the dissociated electrolytes in this solvent were calculated.					
36233049	3	34	theme	enthalpy	673:680	arg1	data					682:685	enthalpy data	673:685	enthalpy data obtained by calorimetry	673:709	Thus, the solution thermodynamics of these compounds in water are reported using enthalpy data obtained by calorimetry.					
36233049	0	35	dep	Pharmaceuticals	25:39	arg1	Aspirin					42:48	Aspirin	42:48	Aspirin	42:48	From Solution Studies of Pharmaceuticals (Aspirin and Related Compounds) to the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N,N-Dimethylformamide.					
36233049	0	35	dep	Pharmaceuticals	25:39	arg1	Compounds					62:70	Related Compounds	54:70	Related Compounds	54:70	From Solution Studies of Pharmaceuticals (Aspirin and Related Compounds) to the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N,N-Dimethylformamide.					
36233049	1	36	from	pharmaceuticals	194:208	arg1	N					296:296	N	296:296	N	296:296	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	1	36	from	pharmaceuticals	194:208	arg1	water					286:290	water	286:290	water	286:290	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	3	37	theme	compounds	635:643	arg1	thermodynamics					611:624	the solution thermodynamics	598:624	the solution thermodynamics of these compounds in water	598:652	Thus, the solution thermodynamics of these compounds in water are reported using enthalpy data obtained by calorimetry.					
36233049	6	38	with	interaction	1003:1013	arg1	β-cyclodextrin					1054:1067	β-cyclodextrin	1054:1067	β-cyclodextrin	1054:1067	The thermodynamics of the interaction of acetylsalicylic acid (aspirin) with β-cyclodextrin in water and DMF is fully discussed, emphasizing the two different processes that take place in water at the two different pHs.					
36233049	6	39	theme	different	1126:1134	arg1	processes					1136:1144	the two different processes	1118:1144	the two different processes that take place in water at the two different pHs	1118:1194	The thermodynamics of the interaction of acetylsalicylic acid (aspirin) with β-cyclodextrin in water and DMF is fully discussed, emphasizing the two different processes that take place in water at the two different pHs.					
36233049	0	40	from	Thermodynamics	80:93	arg1	water					136:140	water	136:140	water	136:140	From Solution Studies of Pharmaceuticals (Aspirin and Related Compounds) to the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N,N-Dimethylformamide.					
36233049	0	40	from	Thermodynamics	80:93	arg1	N					146:146	N	146:146	N	146:146	From Solution Studies of Pharmaceuticals (Aspirin and Related Compounds) to the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N,N-Dimethylformamide.					
36233049	5	41	theme	only	944:947	arg1	data					958:961	only enthalpy data	944:961	only enthalpy data	944:961	As the composition of the solid is not the same as that in solution, the Gibbs energy of the solutions of these compounds could not be obtained; only enthalpy data are reported.					
36233049	1	42	from	behavior	182:189	arg1	N					296:296	N	296:296	N	296:296	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	1	42	from	behavior	182:189	arg1	water					286:290	water	286:290	water	286:290	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	5	43	theme	Gibbs	872:876	arg1	energy					878:883	the Gibbs energy	868:883	the Gibbs energy of the solutions of these compounds	868:919	As the composition of the solid is not the same as that in solution, the Gibbs energy of the solutions of these compounds could not be obtained; only enthalpy data are reported.					
36233049	6	44	from	pHs	1192:1194	arg1	water					1165:1169	water	1165:1169	water at the two different pHs	1165:1194	The thermodynamics of the interaction of acetylsalicylic acid (aspirin) with β-cyclodextrin in water and DMF is fully discussed, emphasizing the two different processes that take place in water at the two different pHs.					
36233049	1	45	theme	4-acetoxybenzoic	233:248	arg1	acid					250:253	4-acetoxybenzoic acid	233:253	4-acetoxybenzoic acid	233:253	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	1	45	theme	4-acetoxybenzoic	233:248	arg1	pharmaceuticals					194:208	pharmaceuticals	194:208	pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N	194:296	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	2	46	from	energies	518:525	arg1	solvent					567:573	this solvent	562:573	this solvent	562:573	Taking into account the formation of ion pairs of these pharmaceuticals in water, the solution Gibbs energies of the dissociated electrolytes in this solvent were calculated.					
36233049	1	47	dep	pharmaceuticals	194:208	arg1	acid					250:253	4-acetoxybenzoic acid	233:253	4-acetoxybenzoic acid	233:253	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	1	47	dep	pharmaceuticals	194:208	arg1	acid					277:280	5-acetylsalicylic acid	259:280	5-acetylsalicylic acid	259:280	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	1	47	dep	pharmaceuticals	194:208	arg1	pharmaceuticals					194:208	pharmaceuticals	194:208	pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N	194:296	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	1	47	dep	pharmaceuticals	194:208	arg1	acid					227:230	acetylsalicylic acid	211:230	acetylsalicylic acid	211:230	The solution behavior of pharmaceuticals (acetylsalicylic acid, 4-acetoxybenzoic acid and 5-acetylsalicylic acid) in water and N,N-Dimethylformamide (DMF) at 298.15 K were investigated through solubility, conductance and calorimetric measurements.					
36233049	6	48	theme	acid	1034:1037	arg1	interaction					1003:1013	the interaction	999:1013	the interaction of acetylsalicylic acid (aspirin) with β-cyclodextrin	999:1067	The thermodynamics of the interaction of acetylsalicylic acid (aspirin) with β-cyclodextrin in water and DMF is fully discussed, emphasizing the two different processes that take place in water at the two different pHs.					
36233049	0	49	theme	Related	54:60	arg1	Compounds					62:70	Related Compounds	54:70	Related Compounds	54:70	From Solution Studies of Pharmaceuticals (Aspirin and Related Compounds) to the Thermodynamics of Aspirin-β-Cyclodextrin Interaction in water and N,N-Dimethylformamide.					
36233049	7	50	theme	Gibbs	1225:1229	arg1	energies					1231:1238	the favorable Gibbs energies	1211:1238	the favorable Gibbs energies for these processes	1211:1258	In all cases, the favorable Gibbs energies for these processes are entropically controlled, mainly resulting from the higher dehydration/desolvation that the receptor undergoes upon interaction with the guest.					
36233049	2	51	theme	ion	454:456	arg1	pairs					458:462	ion pairs	454:462	ion pairs of these pharmaceuticals	454:487	Taking into account the formation of ion pairs of these pharmaceuticals in water, the solution Gibbs energies of the dissociated electrolytes in this solvent were calculated.					
36233049	2	52	from	electrolytes	546:557	arg1	solvent					567:573	this solvent	562:573	this solvent	562:573	Taking into account the formation of ion pairs of these pharmaceuticals in water, the solution Gibbs energies of the dissociated electrolytes in this solvent were calculated.					
37116844	4	0	theme	major	999:1003	arg1	forces					1005:1010	the major forces	995:1010	the major forces maintaining the internal bonding of the composite film	995:1065	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	4	0	theme	major	999:1003	arg1	bonding					1081:1087	hydrogen bonding	1072:1087	hydrogen bonding	1072:1087	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	3	1	theme	higher	778:783	arg1	strength					793:800	higher tensile strength	778:800	higher tensile strength (29.70 Mpa)	778:812	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	3	1	theme	higher	778:783	arg1	Mpa					809:811	29.70 Mpa	803:811	29.70 Mpa	803:811	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	1	2	theme	antibacterial	556:568	arg1	film					570:573	starch-based antibacterial film	543:573	starch-based antibacterial film	543:573	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	7	3	theme	high-performance	1472:1487	arg1	film					1489:1492	This high-performance film	1467:1492	This high-performance film	1467:1492	This high-performance film revealed their potential for packaging materials application.					
37116844	4	4	theme	docking	914:920	arg1	technique					922:930	molecular docking technique	904:930	molecular docking technique	904:930	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	6	5	theme	actual	1382:1387	arg1	food					1389:1392	the actual food	1378:1392	the actual food	1378:1392	Finally, the composite films were used for evaluating the feasibility in the actual food, which largely extended its shelf life compared to the negative control.					
37116844	6	6	theme	composite	1318:1326	arg1	films					1328:1332	the composite films	1314:1332	the composite films	1314:1332	Finally, the composite films were used for evaluating the feasibility in the actual food, which largely extended its shelf life compared to the negative control.					
37116844	2	7	theme	%	632:632	arg1	concentration					611:623	the template SiO2 nanoparticles concentration	579:623	the template SiO2 nanoparticles concentration of 0.1 %	579:632	At the template SiO2 nanoparticles concentration of 0.1 %, the 3D-MPS exhibited anticipated alveolate structure with internal aperture of approximately 10 μm confirmed by SEM.					
37116844	2	8	theme	internal	693:700	arg1	aperture					702:709	internal aperture	693:709	internal aperture of approximately 10 μm confirmed by SEM	693:749	At the template SiO2 nanoparticles concentration of 0.1 %, the 3D-MPS exhibited anticipated alveolate structure with internal aperture of approximately 10 μm confirmed by SEM.					
37116844	1	9	theme	treated	173:179	arg1	material					198:205	a non-enzymatically treated alternative wall material	153:205	a non-enzymatically treated alternative wall material with effective encapsulation properties	153:245	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	1	10	used	used	453:456	arg2	3D-MPS					353:358	3D-MPS	353:358	3D-MPS	353:358	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	1	10	used	used	453:456	arg2	starch					345:350	a novel 3D-micronetwork porous starch	314:350	a novel 3D-micronetwork porous starch (3D-MPS)	314:359	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	0	11	theme	starch-based	103:114	arg1	film					130:133	starch-based antibacterial film	103:133	starch-based antibacterial film	103:133	Green preparation of 3D micronetwork eugenol-encapsuled porous starch for improving the performance of starch-based antibacterial film.					
37116844	4	12	theme	hydrogen	1072:1079	arg1	forces					1005:1010	the major forces	995:1010	the major forces maintaining the internal bonding of the composite film	995:1065	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	4	12	theme	hydrogen	1072:1079	arg1	bonding					1081:1087	hydrogen bonding	1072:1087	hydrogen bonding	1072:1087	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	1	13	theme	conventional	273:284	arg1	plastics					304:311	conventional non-biodegradable plastics	273:311	conventional non-biodegradable plastics	273:311	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	4	14	theme	intermolecular	954:967	arg1	forces					969:974	the intermolecular forces	950:974	the intermolecular forces	950:974	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	6	15	from	feasibility	1363:1373	arg1	food					1389:1392	the actual food	1378:1392	the actual food	1378:1392	Finally, the composite films were used for evaluating the feasibility in the actual food, which largely extended its shelf life compared to the negative control.					
37116844	1	16	theme	film	570:573	arg1	performance					528:538	the performance	524:538	the performance of starch-based antibacterial film	524:573	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	1	17	theme	non-biodegradable	286:302	arg1	plastics					304:311	conventional non-biodegradable plastics	273:311	conventional non-biodegradable plastics	273:311	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	0	18	theme	film	130:133	arg1	performance					88:98	the performance	84:98	the performance of starch-based antibacterial film	84:133	Green preparation of 3D micronetwork eugenol-encapsuled porous starch for improving the performance of starch-based antibacterial film.					
37116844	3	19	theme	3D-EMPS	769:775	arg1	addition					757:764	addition	757:764	addition of 3D-EMPS	757:775	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	2	20	theme	anticipated	656:666	arg1	structure					678:686	anticipated alveolate structure	656:686	anticipated alveolate structure with internal aperture of approximately 10 μm confirmed by SEM	656:749	At the template SiO2 nanoparticles concentration of 0.1 %, the 3D-MPS exhibited anticipated alveolate structure with internal aperture of approximately 10 μm confirmed by SEM.					
37116844	2	21	theme	alveolate	668:676	arg1	structure					678:686	anticipated alveolate structure	656:686	anticipated alveolate structure with internal aperture of approximately 10 μm confirmed by SEM	656:749	At the template SiO2 nanoparticles concentration of 0.1 %, the 3D-MPS exhibited anticipated alveolate structure with internal aperture of approximately 10 μm confirmed by SEM.					
37116844	1	22	theme	alternative	181:191	arg1	material					198:205	a non-enzymatically treated alternative wall material	153:205	a non-enzymatically treated alternative wall material with effective encapsulation properties	153:245	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	0	23	theme	antibacterial	116:128	arg1	film					130:133	starch-based antibacterial film	103:133	starch-based antibacterial film	103:133	Green preparation of 3D micronetwork eugenol-encapsuled porous starch for improving the performance of starch-based antibacterial film.					
37116844	1	24	theme	modified	379:386	arg1	method					409:414	a modified sacrificial template method	377:414	a modified sacrificial template method	377:414	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	6	25	used	used	1339:1342	arg2	films					1328:1332	the composite films	1314:1332	the composite films	1314:1332	Finally, the composite films were used for evaluating the feasibility in the actual food, which largely extended its shelf life compared to the negative control.					
37116844	2	26	theme	10 μm	728:732	arg1	aperture					702:709	internal aperture	693:709	internal aperture of approximately 10 μm confirmed by SEM	693:749	At the template SiO2 nanoparticles concentration of 0.1 %, the 3D-MPS exhibited anticipated alveolate structure with internal aperture of approximately 10 μm confirmed by SEM.					
37116844	6	27	theme	shelf	1422:1426	arg1	life					1428:1431	its shelf life	1418:1431	its shelf life	1418:1431	Finally, the composite films were used for evaluating the feasibility in the actual food, which largely extended its shelf life compared to the negative control.					
37116844	1	28	theme	wall	193:196	arg1	material					198:205	a non-enzymatically treated alternative wall material	153:205	a non-enzymatically treated alternative wall material with effective encapsulation properties	153:245	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	1	29	theme	sacrificial	388:398	arg1	method					409:414	a modified sacrificial template method	377:414	a modified sacrificial template method	377:414	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	1	30	theme	TiO2-starch	478:488	arg1	film					490:493	TiO2-starch film	478:493	TiO2-starch film	478:493	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	4	31	theme	molecular	904:912	arg1	technique					922:930	molecular docking technique	904:930	molecular docking technique	904:930	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	0	32	theme	Green	0:4	arg1	preparation					6:16	Green preparation	0:16	Green preparation of 3D micronetwork	0:35	Green preparation of 3D micronetwork eugenol-encapsuled porous starch for improving the performance of starch-based antibacterial film.					
37116844	1	33	theme	plastics	304:311	arg1	use					266:268	the use	262:268	the use of conventional non-biodegradable plastics	262:311	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	3	34	theme	tensile	785:791	arg1	strength					793:800	higher tensile strength	778:800	higher tensile strength (29.70 Mpa)	778:812	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	3	34	theme	tensile	785:791	arg1	Mpa					809:811	29.70 Mpa	803:811	29.70 Mpa	803:811	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	0	35	theme	3D	21:22	arg1	micronetwork					24:35	3D micronetwork	21:35	3D micronetwork	21:35	Green preparation of 3D micronetwork eugenol-encapsuled porous starch for improving the performance of starch-based antibacterial film.					
37116844	1	36	theme	effective	212:220	arg1	properties					236:245	effective encapsulation properties	212:245	effective encapsulation properties	212:245	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	5	37	theme	expected	1204:1211	arg1	retardation					1221:1231	the expected eugenol retardation	1200:1231	the expected eugenol retardation	1200:1231	Meanwhile, the composite film demonstrated the expected eugenol retardation and antimicrobial capacity against S. aureus, E. coli, and B. subtilis.					
37116844	1	38	theme	template	400:407	arg1	method					409:414	a modified sacrificial template method	377:414	a modified sacrificial template method	377:414	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	1	39	theme	encapsulation	222:234	arg1	properties					236:245	effective encapsulation properties	212:245	effective encapsulation properties	212:245	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	5	40	theme	eugenol	1213:1219	arg1	retardation					1221:1231	the expected eugenol retardation	1200:1231	the expected eugenol retardation	1200:1231	Meanwhile, the composite film demonstrated the expected eugenol retardation and antimicrobial capacity against S. aureus, E. coli, and B. subtilis.					
37116844	1	41	theme	novel	316:320	arg1	3D-MPS					353:358	3D-MPS	353:358	3D-MPS	353:358	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	1	41	theme	novel	316:320	arg1	starch					345:350	a novel 3D-micronetwork porous starch	314:350	a novel 3D-micronetwork porous starch (3D-MPS)	314:359	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	4	42	theme	3D-MPS	1129:1134	arg1	skeleton					1136:1143	3D-MPS skeleton	1129:1143	3D-MPS skeleton	1129:1143	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	0	43	theme	micronetwork	24:35	arg1	preparation					6:16	Green preparation	0:16	Green preparation of 3D micronetwork	0:35	Green preparation of 3D micronetwork eugenol-encapsuled porous starch for improving the performance of starch-based antibacterial film.					
37116844	1	44	theme	3D-micronetwork	322:336	arg1	3D-MPS					353:358	3D-MPS	353:358	3D-MPS	353:358	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	1	44	theme	3D-micronetwork	322:336	arg1	starch					345:350	a novel 3D-micronetwork porous starch	314:350	a novel 3D-micronetwork porous starch (3D-MPS)	314:359	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	4	45	theme	composite	1052:1060	arg1	film					1062:1065	the composite film	1048:1065	the composite film	1048:1065	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	4	46	from	eugenol	1117:1123	arg1	3D-EMPS					1148:1154	3D-EMPS	1148:1154	3D-EMPS	1148:1154	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	4	47	theme	internal	1028:1035	arg1	bonding					1037:1043	the internal bonding	1024:1043	the internal bonding of the composite film	1024:1065	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	1	48	theme	porous	338:343	arg1	3D-MPS					353:358	3D-MPS	353:358	3D-MPS	353:358	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	1	48	theme	porous	338:343	arg1	starch					345:350	a novel 3D-micronetwork porous starch	314:350	a novel 3D-micronetwork porous starch (3D-MPS)	314:359	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	1	49	with	material	198:205	arg1	properties					236:245	effective encapsulation properties	212:245	effective encapsulation properties	212:245	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	5	50	theme	composite	1172:1180	arg1	film					1182:1185	the composite film	1168:1185	the composite film	1168:1185	Meanwhile, the composite film demonstrated the expected eugenol retardation and antimicrobial capacity against S. aureus, E. coli, and B. subtilis.					
37116844	5	50	theme	composite	1172:1180	arg1	Meanwhile					1157:1165	Meanwhile	1157:1165	Meanwhile	1157:1165	Meanwhile, the composite film demonstrated the expected eugenol retardation and antimicrobial capacity against S. aureus, E. coli, and B. subtilis.					
37116844	2	51	theme	nanoparticles	597:609	arg1	concentration					611:623	the template SiO2 nanoparticles concentration	579:623	the template SiO2 nanoparticles concentration of 0.1 %	579:632	At the template SiO2 nanoparticles concentration of 0.1 %, the 3D-MPS exhibited anticipated alveolate structure with internal aperture of approximately 10 μm confirmed by SEM.					
37116844	7	52	theme	packaging	1523:1531	arg1	application					1543:1553	packaging materials application	1523:1553	packaging materials application	1523:1553	This high-performance film revealed their potential for packaging materials application.					
37116844	3	53	theme	film	875:878	arg1	924 g/cm2·24 h					842:855	924 g/cm2·24 h	842:855	924 g/cm2·24 h	842:855	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	3	53	theme	film	875:878	arg1	strength					793:800	higher tensile strength	778:800	higher tensile strength (29.70 Mpa)	778:812	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	3	53	theme	film	875:878	arg1	Mpa					809:811	29.70 Mpa	803:811	29.70 Mpa	803:811	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	3	53	theme	film	875:878	arg1	property					832:839	water barrier property	818:839	water barrier property (924 g/cm2·24 h)	818:856	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	2	54	theme	SiO2	592:595	arg1	nanoparticles					597:609	template SiO2 nanoparticles	583:609	the template SiO2 nanoparticles concentration of 0.1 %	579:632	At the template SiO2 nanoparticles concentration of 0.1 %, the 3D-MPS exhibited anticipated alveolate structure with internal aperture of approximately 10 μm confirmed by SEM.					
37116844	7	55	theme	materials	1533:1541	arg1	application					1543:1553	packaging materials application	1523:1553	packaging materials application	1523:1553	This high-performance film revealed their potential for packaging materials application.					
37116844	3	56	theme	barrier	824:830	arg1	924 g/cm2·24 h					842:855	924 g/cm2·24 h	842:855	924 g/cm2·24 h	842:855	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	3	56	theme	barrier	824:830	arg1	property					832:839	water barrier property	818:839	water barrier property (924 g/cm2·24 h)	818:856	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	2	57	theme	template	583:590	arg1	nanoparticles					597:609	template SiO2 nanoparticles	583:609	the template SiO2 nanoparticles concentration of 0.1 %	579:632	At the template SiO2 nanoparticles concentration of 0.1 %, the 3D-MPS exhibited anticipated alveolate structure with internal aperture of approximately 10 μm confirmed by SEM.					
37116844	4	58	theme	film	1062:1065	arg1	bonding					1037:1043	the internal bonding	1024:1043	the internal bonding of the composite film	1024:1065	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	2	59	with	structure	678:686	arg1	aperture					702:709	internal aperture	693:709	internal aperture of approximately 10 μm confirmed by SEM	693:749	At the template SiO2 nanoparticles concentration of 0.1 %, the 3D-MPS exhibited anticipated alveolate structure with internal aperture of approximately 10 μm confirmed by SEM.					
37116844	6	60	theme	negative	1449:1456	arg1	control					1458:1464	the negative control	1445:1464	the negative control	1445:1464	Finally, the composite films were used for evaluating the feasibility in the actual food, which largely extended its shelf life compared to the negative control.					
37116844	4	61	from	skeleton	1136:1143	arg1	3D-EMPS					1148:1154	3D-EMPS	1148:1154	3D-EMPS	1148:1154	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	3	62	theme	composite	865:873	arg1	film					875:878	the composite film	861:878	the composite film	861:878	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	4	63	used	used	936:939	arg2	technique					922:930	molecular docking technique	904:930	molecular docking technique	904:930	Moreover, molecular docking technique was used to model the intermolecular forces, which showed that the major forces maintaining the internal bonding of the composite film were hydrogen bonding and the interaction between eugenol and 3D-MPS skeleton in 3D-EMPS.					
37116844	5	64	theme	antimicrobial	1237:1249	arg1	capacity					1251:1258	antimicrobial capacity	1237:1258	antimicrobial capacity	1237:1258	Meanwhile, the composite film demonstrated the expected eugenol retardation and antimicrobial capacity against S. aureus, E. coli, and B. subtilis.					
37116844	1	65	theme	starch-based	543:554	arg1	film					570:573	starch-based antibacterial film	543:573	starch-based antibacterial film	543:573	In order to find a non-enzymatically treated alternative wall material with effective encapsulation properties, and to reduce the use of conventional non-biodegradable plastics, a novel 3D-micronetwork porous starch (3D-MPS) was created via a modified sacrificial template method to encapsulate eugenol (3D-EMPS) and used to incorporate with TiO2-starch film, for significantly improving the performance of starch-based antibacterial film.					
37116844	3	66	theme	water	818:822	arg1	924 g/cm2·24 h					842:855	924 g/cm2·24 h	842:855	924 g/cm2·24 h	842:855	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
37116844	3	66	theme	water	818:822	arg1	property					832:839	water barrier property	818:839	water barrier property (924 g/cm2·24 h)	818:856	With addition of 3D-EMPS, higher tensile strength (29.70 Mpa) and water barrier property (924 g/cm2·24 h) of the composite film was obtained.					
35327586	13	0	theme	fluid-based	2280:2290	arg1	methods					2292:2298	supercritical fluid-based methods	2266:2298	supercritical fluid-based methods	2266:2298	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	7	1	theme	amounts	1273:1279	arg1	influence					1202:1210	The influence	1198:1210	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends	1198:1313	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	6	2	theme	support	1181:1187	arg1	polymer					1189:1195	a support polymer	1179:1195	a support polymer	1179:1195	The bioinks containing the extracted ECM were fabricated by combining them with alginate as a support polymer.					
35327586	6	2	theme	support	1181:1187	arg1	them					1157:1160	them	1157:1160	them	1157:1160	The bioinks containing the extracted ECM were fabricated by combining them with alginate as a support polymer.					
35327586	11	3	from	prominent	2001:2009	arg1	samples					2014:2020	samples	2014:2020	samples containing aECM	2014:2036	Furthermore, rheological analysis indicated an increase in viscosity with increasing ECM composition, an effect even more prominent in samples containing aECM.					
35327586	13	4	theme	bioinks	2364:2370	arg1	relevance					2339:2347	the relevance	2335:2347	the relevance of ECM-derived bioinks in the development of printed tissue-like constructs	2335:2423	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	4	5	theme	reactor	822:828	arg1	pressure					806:813	the pressure	802:813	the pressure of the reactor, presence, exposure time, and type of co-solvent	802:877	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	4	6	theme	time	850:853	arg1	pressure					806:813	the pressure	802:813	the pressure of the reactor, presence, exposure time, and type of co-solvent	802:877	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	12	7	theme	3D	2039:2040	arg1	printing					2042:2049	3D printing	2039:2049	3D printing of alginate/ECM constructs	2039:2076	3D printing of alginate/ECM constructs demonstrated that cell viability was only marginally affected by the extrusion process, and this effect was also dependent on the ECM source.					
35327586	2	8	theme	carbon	291:296	arg1	dioxide					298:304	carbon dioxide	291:304	supercritical carbon dioxide (scCO2) technology	277:323	Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks.					
35327586	2	8	theme	carbon	291:296	arg1	scCO2					307:311	scCO2	307:311	scCO2	307:311	Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks.					
35327586	13	9	from	bioinks	2364:2370	arg1	development					2379:2389	the development	2375:2389	the development of printed tissue-like constructs	2375:2423	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	2	10	theme	bioinks	511:517	arg1	preparation					496:506	the preparation	492:506	the preparation of bioinks	492:517	Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks.					
35327586	2	11	theme	3D	425:426	arg1	microenvironments					428:444	accurate 3D microenvironments	416:444	accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks	416:517	Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks.					
35327586	9	12	theme	optimised	1563:1571	arg1	protocol					1579:1586	The optimised scCO2 protocol	1559:1586	The optimised scCO2 protocol	1559:1586	The optimised scCO2 protocol allowed efficient removal of DNA while preserving a higher number of proteins and sGAGs than the standard methodologies.					
35327586	13	13	theme	ECM	2304:2306	arg1	extraction					2308:2317	ECM extraction	2304:2317	ECM extraction	2304:2317	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	5	14	theme	glycosaminoglycans	941:958	arg1	DNA					914:916	DNA	914:916	DNA	914:916	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	14	theme	glycosaminoglycans	941:958	arg1	sGAGs					961:965	sGAGs	961:965	sGAGs	961:965	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	14	theme	glycosaminoglycans	941:958	arg1	amount					904:909	the amount	900:909	the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs)	900:966	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	14	theme	glycosaminoglycans	941:958	arg1	glycosaminoglycans					941:958	sulfated glycosaminoglycans	932:958	sulfated glycosaminoglycans (sGAGs)	932:966	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	14	theme	glycosaminoglycans	941:958	arg1	protein					919:925	protein	919:925	protein	919:925	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	4	15	theme	dermal	678:683	arg1	hDFbs					698:702	hDFbs	698:702	hDFbs	698:702	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	4	15	theme	dermal	678:683	arg1	fibroblasts					685:695	human dermal fibroblasts	672:695	human dermal fibroblasts (hDFbs)	672:703	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	9	16	theme	efficient	1596:1604	arg1	removal					1606:1612	efficient removal	1596:1612	efficient removal of DNA	1596:1619	The optimised scCO2 protocol allowed efficient removal of DNA while preserving a higher number of proteins and sGAGs than the standard methodologies.					
35327586	7	17	theme	2	1233:1233	arg1	%					1234:1234	%	1234:1234	%	1234:1234	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	11	18	from	increase	1926:1933	arg1	viscosity					1938:1946	viscosity	1938:1946	viscosity	1938:1946	Furthermore, rheological analysis indicated an increase in viscosity with increasing ECM composition, an effect even more prominent in samples containing aECM.					
35327586	4	19	theme	Cell	657:660	arg1	sheets					662:667	Cell sheets	657:667	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs)	657:734	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	4	20	dep	reactor	822:828	arg1	the					818:820	the	818:820	the	818:820	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	13	21	theme	tissue-like	2402:2412	arg1	constructs					2414:2423	printed tissue-like constructs	2394:2423	printed tissue-like constructs	2394:2423	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	4	22	theme	adipose	709:715	arg1	hASCs					729:733	hASCs	729:733	hASCs	729:733	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	4	22	theme	adipose	709:715	arg1	cells					722:726	adipose stem cells	709:726	adipose stem cells (hASCs)	709:734	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	10	23	theme	extraction	1857:1866	arg1	protocols					1868:1876	the extraction protocols	1853:1876	the extraction protocols	1853:1876	The characterization of extract's composition also revealed that the ECM produced by hDFbs (fECM) and hASCs (aECM) is distinctively affected by the extraction protocols.					
35327586	13	24	theme	supercritical	2266:2278	arg1	methods					2292:2298	supercritical fluid-based methods	2266:2298	supercritical fluid-based methods	2266:2298	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	13	25	theme	methods	2292:2298	arg1	benefits					2254:2261	the benefits	2250:2261	the benefits of supercritical fluid-based methods for ECM extraction	2250:2317	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	4	26	theme	cells	722:726	arg1	sheets					662:667	Cell sheets	657:667	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs)	657:734	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	9	27	theme	sGAGs	1670:1674	arg1	number					1647:1652	a higher number	1638:1652	a higher number of proteins and sGAGs than the standard methodologies	1638:1706	The optimised scCO2 protocol allowed efficient removal of DNA while preserving a higher number of proteins and sGAGs than the standard methodologies.					
35327586	1	28	theme	matrix	89:94	arg1	bioinks					108:114	Extracellular matrix (ECM)-based bioinks	75:114	Extracellular matrix (ECM)-based bioinks	75:114	Extracellular matrix (ECM)-based bioinks have been steadily gaining interest in the field of bioprinting to develop biologically relevant and functional tissue constructs.					
35327586	11	29	theme	prominent	2001:2009	arg1	composition					1968:1978	ECM composition	1964:1978	ECM composition	1964:1978	Furthermore, rheological analysis indicated an increase in viscosity with increasing ECM composition, an effect even more prominent in samples containing aECM.					
35327586	11	29	theme	prominent	2001:2009	arg1	effect					1984:1989	an effect	1981:1989	an effect even more prominent in samples containing aECM	1981:2036	Furthermore, rheological analysis indicated an increase in viscosity with increasing ECM composition, an effect even more prominent in samples containing aECM.					
35327586	9	30	theme	proteins	1657:1664	arg1	number					1647:1652	a higher number	1638:1652	a higher number of proteins and sGAGs than the standard methodologies	1638:1706	The optimised scCO2 protocol allowed efficient removal of DNA while preserving a higher number of proteins and sGAGs than the standard methodologies.					
35327586	6	31	theme	extracted	1114:1122	arg1	ECM					1124:1126	the extracted ECM	1110:1126	the extracted ECM	1110:1126	The bioinks containing the extracted ECM were fabricated by combining them with alginate as a support polymer.					
35327586	12	32	theme	alginate/ECM	2054:2065	arg1	constructs					2067:2076	alginate/ECM constructs	2054:2076	alginate/ECM constructs	2054:2076	3D printing of alginate/ECM constructs demonstrated that cell viability was only marginally affected by the extrusion process, and this effect was also dependent on the ECM source.					
35327586	7	33	theme	suspensions	1375:1385	arg1	behaviour					1358:1366	the rheological behaviour	1342:1366	the rheological behaviour of the suspensions	1342:1385	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	1	34	theme	-based	101:106	arg1	bioinks					108:114	Extracellular matrix (ECM)-based bioinks	75:114	Extracellular matrix (ECM)-based bioinks	75:114	Extracellular matrix (ECM)-based bioinks have been steadily gaining interest in the field of bioprinting to develop biologically relevant and functional tissue constructs.					
35327586	7	35	theme	alginate	1219:1226	arg1	influence					1202:1210	The influence	1198:1210	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends	1198:1313	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	2	36	theme	own	469:471	arg1	ECM					473:475	the cell's own ECM	458:475	the cell's own ECM	458:475	Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks.					
35327586	8	37	theme	3D	1397:1398	arg1	constructs					1408:1417	3D printed constructs	1397:1417	3D printed constructs	1397:1417	Finally, 3D printed constructs were fabricated using an in-house built extrusion-based bioprinter, and the impact of the extrusion process on cell viability was assessed.					
35327586	11	38	contain	containing	2022:2031	arg2	aECM					2033:2036	aECM	2033:2036	aECM	2033:2036	Furthermore, rheological analysis indicated an increase in viscosity with increasing ECM composition, an effect even more prominent in samples containing aECM.					
35327586	11	38	contain	containing	2022:2031	arg1	samples					2014:2020	samples	2014:2020	samples containing aECM	2014:2036	Furthermore, rheological analysis indicated an increase in viscosity with increasing ECM composition, an effect even more prominent in samples containing aECM.					
35327586	7	39	from	influence	1202:1210	arg1	printability					1288:1299	the printability	1284:1299	the printability of the blends	1284:1313	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	3	40	theme	DNA	613:615	arg1	removal					617:623	efficient DNA removal	603:623	efficient DNA removal	603:623	The ECM extraction protocol best fitted for cell sheets was defined by considering efficient DNA removal with a minor effect on the ECM.					
35327586	4	41	theme	customised	759:768	arg1	system					784:789	a customised supercritical system	757:789	a customised supercritical system	757:789	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	5	42	theme	decellularization	1054:1070	arg1	methodologies					1072:1084	standard decellularization methodologies	1045:1084	standard decellularization methodologies	1045:1084	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	7	43	theme	rheological	1346:1356	arg1	behaviour					1358:1366	the rheological behaviour	1342:1366	the rheological behaviour of the suspensions	1342:1385	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	5	44	theme	sulfated	932:939	arg1	sGAGs					961:965	sGAGs	961:965	sGAGs	961:965	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	44	theme	sulfated	932:939	arg1	glycosaminoglycans					941:958	sulfated glycosaminoglycans	932:958	sulfated glycosaminoglycans (sGAGs)	932:966	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	2	45	theme	technology	314:323	arg1	use					270:272	the use	266:272	the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks	266:517	Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks.					
35327586	1	46	theme	functional	217:226	arg1	constructs					235:244	biologically relevant and functional tissue constructs	191:244	biologically relevant and functional tissue constructs	191:244	Extracellular matrix (ECM)-based bioinks have been steadily gaining interest in the field of bioprinting to develop biologically relevant and functional tissue constructs.					
35327586	9	47	theme	higher	1640:1645	arg1	number					1647:1652	a higher number	1638:1652	a higher number of proteins and sGAGs than the standard methodologies	1638:1706	The optimised scCO2 protocol allowed efficient removal of DNA while preserving a higher number of proteins and sGAGs than the standard methodologies.					
35327586	3	48	theme	minor	632:636	arg1	effect					638:643	a minor effect	630:643	a minor effect on the ECM	630:654	The ECM extraction protocol best fitted for cell sheets was defined by considering efficient DNA removal with a minor effect on the ECM.					
35327586	7	49	dep	alginate	1219:1226	arg1	w/vol					1236:1240	2% w/vol	1233:1240	2% w/vol	1233:1240	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	7	49	dep	alginate	1219:1226	arg1	%					1230:1230	1%	1229:1230	1%	1229:1230	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	8	50	theme	built	1453:1457	arg1	bioprinter					1475:1484	an in-house built extrusion-based bioprinter	1441:1484	an in-house built extrusion-based bioprinter	1441:1484	Finally, 3D printed constructs were fabricated using an in-house built extrusion-based bioprinter, and the impact of the extrusion process on cell viability was assessed.					
35327586	3	51	from	effect	638:643	arg1	ECM					652:654	the ECM	648:654	the ECM	648:654	The ECM extraction protocol best fitted for cell sheets was defined by considering efficient DNA removal with a minor effect on the ECM.					
35327586	2	52	theme	ECM	340:342	arg1	components					344:353	the ECM components	336:353	the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks	336:517	Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks.					
35327586	8	53	theme	process	1519:1525	arg1	impact					1495:1500	the impact	1491:1500	the impact of the extrusion process on cell viability	1491:1543	Finally, 3D printed constructs were fabricated using an in-house built extrusion-based bioprinter, and the impact of the extrusion process on cell viability was assessed.					
35327586	7	54	theme	blends	1308:1313	arg1	printability					1288:1299	the printability	1284:1299	the printability of the blends	1284:1313	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	11	55	theme	ECM	1964:1966	arg1	composition					1968:1978	ECM composition	1964:1978	ECM composition	1964:1978	Furthermore, rheological analysis indicated an increase in viscosity with increasing ECM composition, an effect even more prominent in samples containing aECM.					
35327586	11	55	theme	ECM	1964:1966	arg1	effect					1984:1989	an effect	1981:1989	an effect even more prominent in samples containing aECM	1981:2036	Furthermore, rheological analysis indicated an increase in viscosity with increasing ECM composition, an effect even more prominent in samples containing aECM.					
35327586	13	56	theme	printed	2394:2400	arg1	constructs					2414:2423	printed tissue-like constructs	2394:2423	printed tissue-like constructs	2394:2423	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	2	57	theme	supercritical	277:289	arg1	technology					314:323	supercritical carbon dioxide (scCO2) technology	277:323	supercritical carbon dioxide (scCO2) technology	277:323	Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks.					
35327586	8	58	theme	cell	1530:1533	arg1	viability					1535:1543	cell viability	1530:1543	cell viability	1530:1543	Finally, 3D printed constructs were fabricated using an in-house built extrusion-based bioprinter, and the impact of the extrusion process on cell viability was assessed.					
35327586	12	59	theme	ECM	2208:2210	arg1	source					2212:2217	the ECM source	2204:2217	the ECM source	2204:2217	3D printing of alginate/ECM constructs demonstrated that cell viability was only marginally affected by the extrusion process, and this effect was also dependent on the ECM source.					
35327586	13	60	theme	ECM-derived	2352:2362	arg1	bioinks					2364:2370	ECM-derived bioinks	2352:2370	ECM-derived bioinks in the development of printed tissue-like constructs	2352:2423	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	4	61	theme	supercritical	770:782	arg1	system					784:789	a customised supercritical system	757:789	a customised supercritical system	757:789	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	1	62	theme	bioprinting	168:178	arg1	field					159:163	the field	155:163	the field of bioprinting	155:178	Extracellular matrix (ECM)-based bioinks have been steadily gaining interest in the field of bioprinting to develop biologically relevant and functional tissue constructs.					
35327586	2	63	theme	dioxide	298:304	arg1	technology					314:323	supercritical carbon dioxide (scCO2) technology	277:323	supercritical carbon dioxide (scCO2) technology	277:323	Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks.					
35327586	4	64	theme	exposure	841:848	arg1	time					850:853	exposure time	841:853	exposure time	841:853	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	3	65	theme	cell	564:567	arg1	sheets					569:574	cell sheets	564:574	cell sheets	564:574	The ECM extraction protocol best fitted for cell sheets was defined by considering efficient DNA removal with a minor effect on the ECM.					
35327586	1	66	theme	relevant	204:211	arg1	constructs					235:244	biologically relevant and functional tissue constructs	191:244	biologically relevant and functional tissue constructs	191:244	Extracellular matrix (ECM)-based bioinks have been steadily gaining interest in the field of bioprinting to develop biologically relevant and functional tissue constructs.					
35327586	13	67	from	development	2379:2389	arg1	relevance					2339:2347	the relevance	2335:2347	the relevance of ECM-derived bioinks in the development of printed tissue-like constructs	2335:2423	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	9	68	theme	scCO2	1573:1577	arg1	protocol					1579:1586	The optimised scCO2 protocol	1559:1586	The optimised scCO2 protocol	1559:1586	The optimised scCO2 protocol allowed efficient removal of DNA while preserving a higher number of proteins and sGAGs than the standard methodologies.					
35327586	7	69	theme	ECM	1247:1249	arg1	amounts					1273:1279	the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts	1215:1279	amounts	1273:1279	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	7	70	dep	amounts	1273:1279	arg1	%					1255:1255	0.5%	1252:1255	0.5%	1252:1255	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	7	70	dep	amounts	1273:1279	arg1	%					1264:1264	1.5%	1261:1264	1.5%	1261:1264	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	3	71	theme	ECM	524:526	arg1	protocol					539:546	The ECM extraction protocol	520:546	The ECM extraction protocol best fitted for cell sheets	520:574	The ECM extraction protocol best fitted for cell sheets was defined by considering efficient DNA removal with a minor effect on the ECM.					
35327586	4	72	theme	presence	831:838	arg1	pressure					806:813	the pressure	802:813	the pressure of the reactor, presence, exposure time, and type of co-solvent	802:877	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	4	73	theme	human	672:676	arg1	hDFbs					698:702	hDFbs	698:702	hDFbs	698:702	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	4	73	theme	human	672:676	arg1	fibroblasts					685:695	human dermal fibroblasts	672:695	human dermal fibroblasts (hDFbs)	672:703	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	11	74	theme	rheological	1892:1902	arg1	analysis					1904:1911	rheological analysis	1892:1911	rheological analysis	1892:1911	Furthermore, rheological analysis indicated an increase in viscosity with increasing ECM composition, an effect even more prominent in samples containing aECM.					
35327586	1	75	theme	gaining	135:141	arg1	interest					143:150	steadily gaining interest	126:150	steadily gaining interest in the field of bioprinting	126:178	Extracellular matrix (ECM)-based bioinks have been steadily gaining interest in the field of bioprinting to develop biologically relevant and functional tissue constructs.					
35327586	7	76	dep	%	1255:1255	arg1	w/vol					1266:1270	w/vol	1266:1270	w/vol	1266:1270	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	7	77	theme	%	1234:1234	arg1	w/vol					1236:1240	2% w/vol	1233:1240	2% w/vol	1233:1240	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	7	77	theme	%	1234:1234	arg1	%					1230:1230	1%	1229:1230	1%	1229:1230	The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions.					
35327586	5	78	theme	extraction	1019:1028	arg1	efficiency					1001:1010	the efficiency	997:1010	the efficiency of the extraction in relation to standard decellularization methodologies	997:1084	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	13	79	from	relevance	2339:2347	arg1	development					2379:2389	the development	2375:2389	the development of printed tissue-like constructs	2375:2423	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	9	80	theme	DNA	1617:1619	arg1	removal					1606:1612	efficient removal	1596:1612	efficient removal of DNA	1596:1619	The optimised scCO2 protocol allowed efficient removal of DNA while preserving a higher number of proteins and sGAGs than the standard methodologies.					
35327586	12	81	theme	extrusion	2147:2155	arg1	process					2157:2163	the extrusion process	2143:2163	the extrusion process	2143:2163	3D printing of alginate/ECM constructs demonstrated that cell viability was only marginally affected by the extrusion process, and this effect was also dependent on the ECM source.					
35327586	3	82	theme	extraction	528:537	arg1	protocol					539:546	The ECM extraction protocol	520:546	The ECM extraction protocol best fitted for cell sheets	520:574	The ECM extraction protocol best fitted for cell sheets was defined by considering efficient DNA removal with a minor effect on the ECM.					
35327586	4	83	theme	type	860:863	arg1	pressure					806:813	the pressure	802:813	the pressure of the reactor, presence, exposure time, and type of co-solvent	802:877	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	12	84	theme	constructs	2067:2076	arg1	printing					2042:2049	3D printing	2039:2049	3D printing of alginate/ECM constructs	2039:2076	3D printing of alginate/ECM constructs demonstrated that cell viability was only marginally affected by the extrusion process, and this effect was also dependent on the ECM source.					
35327586	1	85	from	interest	143:150	arg1	field					159:163	the field	155:163	the field of bioprinting	155:178	Extracellular matrix (ECM)-based bioinks have been steadily gaining interest in the field of bioprinting to develop biologically relevant and functional tissue constructs.					
35327586	1	86	theme	Extracellular	75:87	arg1	ECM					97:99	ECM	97:99	ECM	97:99	Extracellular matrix (ECM)-based bioinks have been steadily gaining interest in the field of bioprinting to develop biologically relevant and functional tissue constructs.					
35327586	1	86	theme	Extracellular	75:87	arg1	matrix					89:94	Extracellular matrix	75:94	Extracellular matrix (ECM)-based bioinks	75:114	Extracellular matrix (ECM)-based bioinks have been steadily gaining interest in the field of bioprinting to develop biologically relevant and functional tissue constructs.					
35327586	4	87	theme	co-solvent	868:877	arg1	presence					831:838	presence	831:838	presence	831:838	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	4	87	theme	co-solvent	868:877	arg1	time					850:853	exposure time	841:853	exposure time	841:853	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	4	87	theme	co-solvent	868:877	arg1	reactor					822:828	reactor	822:828	reactor	822:828	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	4	87	theme	co-solvent	868:877	arg1	type					860:863	type	860:863	type	860:863	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	4	88	theme	stem	717:720	arg1	hASCs					729:733	hASCs	729:733	hASCs	729:733	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	4	88	theme	stem	717:720	arg1	cells					722:726	adipose stem cells	709:726	adipose stem cells (hASCs)	709:734	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	5	89	from	efficiency	1001:1010	arg1	relation					1033:1040	relation	1033:1040	relation to standard decellularization methodologies	1033:1084	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	13	90	link	ECM-derived	2352:2362	arg1	bioinks					2364:2370	ECM-derived bioinks	2352:2370	ECM-derived bioinks in the development of printed tissue-like constructs	2352:2423	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	4	91	theme	fibroblasts	685:695	arg1	sheets					662:667	Cell sheets	657:667	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs)	657:734	Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent.					
35327586	2	92	theme	cell-sheets	358:368	arg1	components					344:353	the ECM components	336:353	the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks	336:517	Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks.					
35327586	0	93	theme	ECM	22:24	arg1	Components					26:35	ECM Components	22:35	ECM Components Obtained by Supercritical Extraction	22:72	Bioinks Enriched with ECM Components Obtained by Supercritical Extraction.					
35327586	2	94	theme	accurate	416:423	arg1	microenvironments					428:444	accurate 3D microenvironments	416:444	accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks	416:517	Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks.					
35327586	8	95	theme	printed	1400:1406	arg1	constructs					1408:1417	3D printed constructs	1397:1417	3D printed constructs	1397:1417	Finally, 3D printed constructs were fabricated using an in-house built extrusion-based bioprinter, and the impact of the extrusion process on cell viability was assessed.					
35327586	12	96	theme	cell	2096:2099	arg1	viability					2101:2109	cell viability	2096:2109	cell viability	2096:2109	3D printing of alginate/ECM constructs demonstrated that cell viability was only marginally affected by the extrusion process, and this effect was also dependent on the ECM source.					
35327586	13	97	theme	constructs	2414:2423	arg1	development					2379:2389	the development	2375:2389	the development of printed tissue-like constructs	2375:2423	Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.					
35327586	5	98	theme	protein	919:925	arg1	DNA					914:916	DNA	914:916	DNA	914:916	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	98	theme	protein	919:925	arg1	sGAGs					961:965	sGAGs	961:965	sGAGs	961:965	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	98	theme	protein	919:925	arg1	amount					904:909	the amount	900:909	the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs)	900:966	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	98	theme	protein	919:925	arg1	glycosaminoglycans					941:958	sulfated glycosaminoglycans	932:958	sulfated glycosaminoglycans (sGAGs)	932:966	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	98	theme	protein	919:925	arg1	protein					919:925	protein	919:925	protein	919:925	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	3	99	theme	efficient	603:611	arg1	removal					617:623	efficient DNA removal	603:623	efficient DNA removal	603:623	The ECM extraction protocol best fitted for cell sheets was defined by considering efficient DNA removal with a minor effect on the ECM.					
35327586	2	100	theme	promising	386:394	arg1	results					396:402	promising results	386:402	promising results	386:402	Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell's own ECM, to be used in the preparation of bioinks.					
35327586	0	101	theme	Supercritical	49:61	arg1	Extraction					63:72	Supercritical Extraction	49:72	Supercritical Extraction	49:72	Bioinks Enriched with ECM Components Obtained by Supercritical Extraction.					
35327586	5	102	theme	standard	1045:1052	arg1	methodologies					1072:1084	standard decellularization methodologies	1045:1084	standard decellularization methodologies	1045:1084	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	9	103	theme	standard	1685:1692	arg1	methodologies					1694:1706	the standard methodologies	1681:1706	the standard methodologies	1681:1706	The optimised scCO2 protocol allowed efficient removal of DNA while preserving a higher number of proteins and sGAGs than the standard methodologies.					
35327586	10	104	theme	composition	1743:1753	arg1	characterization					1713:1728	The characterization	1709:1728	The characterization of extract's composition	1709:1753	The characterization of extract's composition also revealed that the ECM produced by hDFbs (fECM) and hASCs (aECM) is distinctively affected by the extraction protocols.					
35327586	5	105	theme	amount	904:909	arg1	quantification					882:895	A quantification	880:895	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs)	880:966	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	8	106	theme	in-house	1444:1451	arg1	bioprinter					1475:1484	an in-house built extrusion-based bioprinter	1441:1484	an in-house built extrusion-based bioprinter	1441:1484	Finally, 3D printed constructs were fabricated using an in-house built extrusion-based bioprinter, and the impact of the extrusion process on cell viability was assessed.					
35327586	8	107	from	impact	1495:1500	arg1	viability					1535:1543	cell viability	1530:1543	cell viability	1530:1543	Finally, 3D printed constructs were fabricated using an in-house built extrusion-based bioprinter, and the impact of the extrusion process on cell viability was assessed.					
35327586	8	108	theme	extrusion	1509:1517	arg1	process					1519:1525	the extrusion process	1505:1525	the extrusion process	1505:1525	Finally, 3D printed constructs were fabricated using an in-house built extrusion-based bioprinter, and the impact of the extrusion process on cell viability was assessed.					
35327586	1	109	theme	tissue	228:233	arg1	constructs					235:244	biologically relevant and functional tissue constructs	191:244	biologically relevant and functional tissue constructs	191:244	Extracellular matrix (ECM)-based bioinks have been steadily gaining interest in the field of bioprinting to develop biologically relevant and functional tissue constructs.					
35327586	5	110	theme	DNA	914:916	arg1	DNA					914:916	DNA	914:916	DNA	914:916	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	110	theme	DNA	914:916	arg1	sGAGs					961:965	sGAGs	961:965	sGAGs	961:965	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	110	theme	DNA	914:916	arg1	amount					904:909	the amount	900:909	the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs)	900:966	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	110	theme	DNA	914:916	arg1	glycosaminoglycans					941:958	sulfated glycosaminoglycans	932:958	sulfated glycosaminoglycans (sGAGs)	932:966	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	5	110	theme	DNA	914:916	arg1	protein					919:925	protein	919:925	protein	919:925	A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies.					
35327586	8	111	theme	extrusion-based	1459:1473	arg1	bioprinter					1475:1484	an in-house built extrusion-based bioprinter	1441:1484	an in-house built extrusion-based bioprinter	1441:1484	Finally, 3D printed constructs were fabricated using an in-house built extrusion-based bioprinter, and the impact of the extrusion process on cell viability was assessed.					
35327586	11	112	from	samples	2014:2020	arg1	prominent					2001:2009	prominent	2001:2009	prominent	2001:2009	Furthermore, rheological analysis indicated an increase in viscosity with increasing ECM composition, an effect even more prominent in samples containing aECM.					
35327586	6	113	contain	containing	1099:1108	arg1	bioinks					1091:1097	The bioinks	1087:1097	The bioinks containing the extracted ECM	1087:1126	The bioinks containing the extracted ECM were fabricated by combining them with alginate as a support polymer.					
35327586	6	113	contain	containing	1099:1108	arg2	ECM					1124:1126	the extracted ECM	1110:1126	the extracted ECM	1110:1126	The bioinks containing the extracted ECM were fabricated by combining them with alginate as a support polymer.					
34999048	0	0	theme	silver	91:96	arg1	nanoparticles					98:110	immobilized silver nanoparticles	79:110	immobilized silver nanoparticles	79:110	High antibacterial activity of chitosan films with covalent organic frameworks immobilized silver nanoparticles.					
34999048	2	1	theme	in-situ	359:365	arg1	synthesis					367:375	in-situ synthesis	359:375	in-situ synthesis of immobilizing AgNPs on COFs	359:405	COFs-AgNPs were fabricated via in-situ synthesis of immobilizing AgNPs on COFs.					
34999048	0	2	theme	immobilized	79:89	arg1	nanoparticles					98:110	immobilized silver nanoparticles	79:110	immobilized silver nanoparticles	79:110	High antibacterial activity of chitosan films with covalent organic frameworks immobilized silver nanoparticles.					
34999048	5	3	theme	COFs-AgNPs	939:948	arg1	content					950:956	the COFs-AgNPs content	935:956	the COFs-AgNPs content	935:956	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	6	4	theme	Carassius	1272:1280	arg1	carp					1266:1269	white crucian carp	1252:1269	white crucian carp (Carassius auratus)	1252:1289	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	6	4	theme	Carassius	1272:1280	arg1	auratus					1282:1288	Carassius auratus	1272:1288	Carassius auratus	1272:1288	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	4	5	theme	solution	794:801	arg1	method					811:816	a solution casting method	792:816	a solution casting method	792:816	Furthermore, the as-prepared COFs-AgNPs composite was further used to fabricate CS composite films (CS/COFs-AgNPs) by a solution casting method.					
34999048	4	6	theme	as-prepared	691:701	arg1	composite					714:722	the as-prepared COFs-AgNPs composite	687:722	the as-prepared COFs-AgNPs composite	687:722	Furthermore, the as-prepared COFs-AgNPs composite was further used to fabricate CS composite films (CS/COFs-AgNPs) by a solution casting method.					
34999048	1	7	contain	containing	147:156	arg1	film					142:145	chitosan (CS) film	128:145	chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs)	128:232	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	1	7	contain	containing	147:156	arg2	COFs					187:190	COFs	187:190	COFs	187:190	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	1	7	contain	containing	147:156	arg2	frameworks					175:184	covalent organic frameworks	158:184	covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs)	158:232	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	5	8	theme	water	1004:1008	arg1	swelling					1010:1017	water swelling	1004:1017	water swelling	1004:1017	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	3	9	theme	COFs-AgNPs	577:586	arg1	fabrication					562:572	the successful fabrication	547:572	the successful fabrication of COFs-AgNPs	547:586	Transmission electron microscope, Zeta potential, X-ray diffraction, element mapping and Fourier transform infrared spectroscopy confirmed the successful fabrication of COFs-AgNPs, and COFs-AgNPs showed superior antibacterial activity against S. aureus and E. coli.					
34999048	1	10	theme	food	253:256	arg1	packaging					258:266	food packaging	253:266	food packaging with improved antibacterial activities and film properties	253:325	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	5	11	theme	nanocomposite	872:884	arg1	films					886:890	the nanocomposite films	868:890	the nanocomposite films	868:890	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	5	12	theme	content	950:956	arg1	increase					923:930	the increase	919:930	the increase of the COFs-AgNPs content	919:956	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	3	13	theme	successful	551:560	arg1	fabrication					562:572	the successful fabrication	547:572	the successful fabrication of COFs-AgNPs	547:586	Transmission electron microscope, Zeta potential, X-ray diffraction, element mapping and Fourier transform infrared spectroscopy confirmed the successful fabrication of COFs-AgNPs, and COFs-AgNPs showed superior antibacterial activity against S. aureus and E. coli.					
34999048	7	14	theme	active	1363:1368	arg1	packaging					1375:1383	active food packaging	1363:1383	active food packaging	1363:1383	As a result, CS/COFs-AgNPs nanocomposite films show great potential in active food packaging.					
34999048	1	15	theme	covalent	158:165	arg1	frameworks					175:184	covalent organic frameworks	158:184	covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs)	158:232	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	1	15	theme	covalent	158:165	arg1	COFs					187:190	COFs	187:190	COFs	187:190	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	3	16	theme	electron	421:428	arg1	microscope					430:439	Transmission electron microscope	408:439	Transmission electron microscope	408:439	Transmission electron microscope, Zeta potential, X-ray diffraction, element mapping and Fourier transform infrared spectroscopy confirmed the successful fabrication of COFs-AgNPs, and COFs-AgNPs showed superior antibacterial activity against S. aureus and E. coli.					
34999048	4	17	used	used	736:739	arg2	composite					714:722	the as-prepared COFs-AgNPs composite	687:722	the as-prepared COFs-AgNPs composite	687:722	Furthermore, the as-prepared COFs-AgNPs composite was further used to fabricate CS composite films (CS/COFs-AgNPs) by a solution casting method.					
34999048	0	18	dep	nanoparticles	98:110	arg1	activity					19:26	High antibacterial activity	0:26	High antibacterial activity of chitosan films with covalent organic frameworks	0:77	High antibacterial activity of chitosan films with covalent organic frameworks immobilized silver nanoparticles.					
34999048	1	19	theme	organic	167:173	arg1	frameworks					175:184	covalent organic frameworks	158:184	covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs)	158:232	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	1	19	theme	organic	167:173	arg1	COFs					187:190	COFs	187:190	COFs	187:190	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	0	20	theme	antibacterial	5:17	arg1	activity					19:26	High antibacterial activity	0:26	High antibacterial activity of chitosan films with covalent organic frameworks	0:77	High antibacterial activity of chitosan films with covalent organic frameworks immobilized silver nanoparticles.					
34999048	5	21	theme	UV-visible	969:978	arg1	barrier					986:992	the UV-visible light barrier	965:992	the UV-visible light barrier property	965:1001	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	7	22	theme	CS/COFs-AgNPs	1305:1317	arg1	films					1333:1337	CS/COFs-AgNPs nanocomposite films	1305:1337	CS/COFs-AgNPs nanocomposite films	1305:1337	As a result, CS/COFs-AgNPs nanocomposite films show great potential in active food packaging.					
34999048	2	23	theme	AgNPs	393:397	arg1	synthesis					367:375	in-situ synthesis	359:375	in-situ synthesis of immobilizing AgNPs on COFs	359:405	COFs-AgNPs were fabricated via in-situ synthesis of immobilizing AgNPs on COFs.					
34999048	0	24	theme	High	0:3	arg1	activity					19:26	High antibacterial activity	0:26	High antibacterial activity of chitosan films with covalent organic frameworks	0:77	High antibacterial activity of chitosan films with covalent organic frameworks immobilized silver nanoparticles.					
34999048	1	25	with	packaging	258:266	arg1	activities					296:305	improved antibacterial activities	273:305	improved antibacterial activities	273:305	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	1	25	with	packaging	258:266	arg1	properties					316:325	film properties	311:325	film properties	311:325	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	5	26	theme	light	980:984	arg1	barrier					986:992	the UV-visible light barrier	965:992	the UV-visible light barrier property	965:1001	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	2	27	theme	immobilizing	380:391	arg1	AgNPs					393:397	immobilizing AgNPs	380:397	immobilizing AgNPs on COFs	380:405	COFs-AgNPs were fabricated via in-situ synthesis of immobilizing AgNPs on COFs.					
34999048	5	28	theme	tensile	848:854	arg1	strength					856:863	the tensile strength	844:863	the tensile strength of the nanocomposite films	844:890	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	2	29	from	synthesis	367:375	arg1	COFs					402:405	COFs	402:405	COFs	402:405	COFs-AgNPs were fabricated via in-situ synthesis of immobilizing AgNPs on COFs.					
34999048	5	30	theme	water	1050:1054	arg1	WVP					1076:1078	WVP	1076:1078	WVP	1076:1078	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	5	30	theme	water	1050:1054	arg1	permeability					1062:1073	water vapor permeability	1050:1073	water vapor permeability (WVP)	1050:1079	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	7	31	theme	great	1344:1348	arg1	potential					1350:1358	great potential	1344:1358	great potential	1344:1358	As a result, CS/COFs-AgNPs nanocomposite films show great potential in active food packaging.					
34999048	7	31	theme	great	1344:1348	arg1	result					1297:1302	a result	1295:1302	a result	1295:1302	As a result, CS/COFs-AgNPs nanocomposite films show great potential in active food packaging.					
34999048	1	32	theme	improved	273:280	arg1	activities					296:305	improved antibacterial activities	273:305	improved antibacterial activities	273:305	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	3	33	dep	Fourier	497:503	arg1	transform					505:513	transform	505:513	transform infrared spectroscopy	505:535	Transmission electron microscope, Zeta potential, X-ray diffraction, element mapping and Fourier transform infrared spectroscopy confirmed the successful fabrication of COFs-AgNPs, and COFs-AgNPs showed superior antibacterial activity against S. aureus and E. coli.					
34999048	6	34	theme	antibacterial	1183:1195	arg1	activity					1197:1204	outstanding antibacterial activity	1171:1204	outstanding antibacterial activity	1171:1204	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	3	35	theme	element	477:483	arg1	mapping					485:491	element mapping	477:491	element mapping	477:491	Transmission electron microscope, Zeta potential, X-ray diffraction, element mapping and Fourier transform infrared spectroscopy confirmed the successful fabrication of COFs-AgNPs, and COFs-AgNPs showed superior antibacterial activity against S. aureus and E. coli.					
34999048	0	36	theme	films	40:44	arg1	activity					19:26	High antibacterial activity	0:26	High antibacterial activity of chitosan films with covalent organic frameworks	0:77	High antibacterial activity of chitosan films with covalent organic frameworks immobilized silver nanoparticles.					
34999048	1	37	theme	antibacterial	282:294	arg1	activities					296:305	improved antibacterial activities	273:305	improved antibacterial activities	273:305	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	3	38	theme	X-ray	458:462	arg1	diffraction					464:474	X-ray diffraction	458:474	X-ray diffraction	458:474	Transmission electron microscope, Zeta potential, X-ray diffraction, element mapping and Fourier transform infrared spectroscopy confirmed the successful fabrication of COFs-AgNPs, and COFs-AgNPs showed superior antibacterial activity against S. aureus and E. coli.					
34999048	1	39	dep	frameworks	175:184	arg1	AgNPs					227:231	AgNPs	227:231	AgNPs	227:231	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	1	39	dep	frameworks	175:184	arg1	nanoparticles					212:224	immobilized silver nanoparticles	193:224	covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs)	158:232	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	3	40	theme	Zeta	442:445	arg1	potential					447:455	Zeta potential	442:455	Zeta potential	442:455	Transmission electron microscope, Zeta potential, X-ray diffraction, element mapping and Fourier transform infrared spectroscopy confirmed the successful fabrication of COFs-AgNPs, and COFs-AgNPs showed superior antibacterial activity against S. aureus and E. coli.					
34999048	1	41	theme	immobilized	193:203	arg1	AgNPs					227:231	AgNPs	227:231	AgNPs	227:231	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	1	41	theme	immobilized	193:203	arg1	nanoparticles					212:224	immobilized silver nanoparticles	193:224	covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs)	158:232	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	0	42	theme	chitosan	31:38	arg1	films					40:44	chitosan films	31:44	chitosan films	31:44	High antibacterial activity of chitosan films with covalent organic frameworks immobilized silver nanoparticles.					
34999048	6	43	theme	storage	1236:1242	arg1	time					1244:1247	the storage time	1232:1247	the storage time of white crucian carp (Carassius auratus)	1232:1289	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	5	44	theme	vapor	1056:1060	arg1	WVP					1076:1078	WVP	1076:1078	WVP	1076:1078	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	5	44	theme	vapor	1056:1060	arg1	permeability					1062:1073	water vapor permeability	1050:1073	water vapor permeability (WVP)	1050:1079	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	2	45	from	AgNPs	393:397	arg1	COFs					402:405	COFs	402:405	COFs	402:405	COFs-AgNPs were fabricated via in-situ synthesis of immobilizing AgNPs on COFs.					
34999048	1	46	theme	silver	205:210	arg1	AgNPs					227:231	AgNPs	227:231	AgNPs	227:231	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	1	46	theme	silver	205:210	arg1	nanoparticles					212:224	immobilized silver nanoparticles	193:224	covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs)	158:232	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	0	47	theme	covalent	51:58	arg1	frameworks					68:77	covalent organic frameworks	51:77	covalent organic frameworks	51:77	High antibacterial activity of chitosan films with covalent organic frameworks immobilized silver nanoparticles.					
34999048	3	48	theme	infrared	515:522	arg1	spectroscopy					524:535	infrared spectroscopy	515:535	infrared spectroscopy	515:535	Transmission electron microscope, Zeta potential, X-ray diffraction, element mapping and Fourier transform infrared spectroscopy confirmed the successful fabrication of COFs-AgNPs, and COFs-AgNPs showed superior antibacterial activity against S. aureus and E. coli.					
34999048	6	49	theme	nanocomposite	1139:1151	arg1	films					1153:1157	the CS/COFs-AgNPs nanocomposite films	1121:1157	the CS/COFs-AgNPs nanocomposite films	1121:1157	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	7	50	theme	nanocomposite	1319:1331	arg1	films					1333:1337	CS/COFs-AgNPs nanocomposite films	1305:1337	CS/COFs-AgNPs nanocomposite films	1305:1337	As a result, CS/COFs-AgNPs nanocomposite films show great potential in active food packaging.					
34999048	3	51	theme	superior	611:618	arg1	activity					634:641	superior antibacterial activity	611:641	superior antibacterial activity against S. aureus and E. coli	611:671	Transmission electron microscope, Zeta potential, X-ray diffraction, element mapping and Fourier transform infrared spectroscopy confirmed the successful fabrication of COFs-AgNPs, and COFs-AgNPs showed superior antibacterial activity against S. aureus and E. coli.					
34999048	0	52	with	activity	19:26	arg1	frameworks					68:77	covalent organic frameworks	51:77	covalent organic frameworks	51:77	High antibacterial activity of chitosan films with covalent organic frameworks immobilized silver nanoparticles.					
34999048	4	53	theme	CS	754:755	arg1	CS/COFs-AgNPs					774:786	CS/COFs-AgNPs	774:786	CS/COFs-AgNPs	774:786	Furthermore, the as-prepared COFs-AgNPs composite was further used to fabricate CS composite films (CS/COFs-AgNPs) by a solution casting method.					
34999048	4	53	theme	CS	754:755	arg1	films					767:771	CS composite films	754:771	CS composite films (CS/COFs-AgNPs)	754:787	Furthermore, the as-prepared COFs-AgNPs composite was further used to fabricate CS composite films (CS/COFs-AgNPs) by a solution casting method.					
34999048	3	54	theme	Transmission	408:419	arg1	microscope					430:439	Transmission electron microscope	408:439	Transmission electron microscope	408:439	Transmission electron microscope, Zeta potential, X-ray diffraction, element mapping and Fourier transform infrared spectroscopy confirmed the successful fabrication of COFs-AgNPs, and COFs-AgNPs showed superior antibacterial activity against S. aureus and E. coli.					
34999048	1	55	theme	film	311:314	arg1	properties					316:325	film properties	311:325	film properties	311:325	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	7	56	theme	food	1370:1373	arg1	packaging					1375:1383	active food packaging	1363:1383	active food packaging	1363:1383	As a result, CS/COFs-AgNPs nanocomposite films show great potential in active food packaging.					
34999048	6	57	dep	only	1110:1113	arg1	that					1115:1118	that	1115:1118	that	1115:1118	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	3	58	theme	antibacterial	620:632	arg1	activity					634:641	superior antibacterial activity	611:641	superior antibacterial activity against S. aureus and E. coli	611:671	Transmission electron microscope, Zeta potential, X-ray diffraction, element mapping and Fourier transform infrared spectroscopy confirmed the successful fabrication of COFs-AgNPs, and COFs-AgNPs showed superior antibacterial activity against S. aureus and E. coli.					
34999048	2	59	from	COFs	402:405	arg1	synthesis					367:375	in-situ synthesis	359:375	in-situ synthesis of immobilizing AgNPs on COFs	359:405	COFs-AgNPs were fabricated via in-situ synthesis of immobilizing AgNPs on COFs.					
34999048	4	60	theme	COFs-AgNPs	703:712	arg1	composite					714:722	the as-prepared COFs-AgNPs composite	687:722	the as-prepared COFs-AgNPs composite	687:722	Furthermore, the as-prepared COFs-AgNPs composite was further used to fabricate CS composite films (CS/COFs-AgNPs) by a solution casting method.					
34999048	6	61	theme	carp	1266:1269	arg1	time					1244:1247	the storage time	1232:1247	the storage time of white crucian carp (Carassius auratus)	1232:1289	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	5	62	theme	solubility	1023:1032	arg1	properties					1034:1043	solubility properties	1023:1043	solubility properties	1023:1043	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	1	63	theme	chitosan	128:135	arg1	film					142:145	chitosan (CS) film	128:145	chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs)	128:232	In this study, chitosan (CS) film containing covalent organic frameworks (COFs) immobilized silver nanoparticles (AgNPs) were developed for food packaging with improved antibacterial activities and film properties.					
34999048	4	64	theme	casting	803:809	arg1	method					811:816	a solution casting method	792:816	a solution casting method	792:816	Furthermore, the as-prepared COFs-AgNPs composite was further used to fabricate CS composite films (CS/COFs-AgNPs) by a solution casting method.					
34999048	6	65	theme	crucian	1258:1264	arg1	carp					1266:1269	white crucian carp	1252:1269	white crucian carp (Carassius auratus)	1252:1289	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	6	65	theme	crucian	1258:1264	arg1	auratus					1282:1288	Carassius auratus	1272:1288	Carassius auratus	1272:1288	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	4	66	theme	composite	757:765	arg1	CS/COFs-AgNPs					774:786	CS/COFs-AgNPs	774:786	CS/COFs-AgNPs	774:786	Furthermore, the as-prepared COFs-AgNPs composite was further used to fabricate CS composite films (CS/COFs-AgNPs) by a solution casting method.					
34999048	4	66	theme	composite	757:765	arg1	films					767:771	CS composite films	754:771	CS composite films (CS/COFs-AgNPs)	754:787	Furthermore, the as-prepared COFs-AgNPs composite was further used to fabricate CS composite films (CS/COFs-AgNPs) by a solution casting method.					
34999048	6	67	theme	outstanding	1171:1181	arg1	activity					1197:1204	outstanding antibacterial activity	1171:1204	outstanding antibacterial activity	1171:1204	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	5	68	theme	films	886:890	arg1	strength					856:863	the tensile strength	844:863	the tensile strength of the nanocomposite films	844:890	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	6	69	theme	white	1252:1256	arg1	carp					1266:1269	white crucian carp	1252:1269	white crucian carp (Carassius auratus)	1252:1289	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	6	69	theme	white	1252:1256	arg1	auratus					1282:1288	Carassius auratus	1272:1288	Carassius auratus	1272:1288	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	5	70	dep	barrier	986:992	arg1	property					994:1001	property	994:1001	the UV-visible light barrier property	965:1001	The findings showed that the tensile strength of the nanocomposite films enhanced dramatically with the increase of the COFs-AgNPs content, while the UV-visible light barrier property, water swelling and solubility properties, and water vapor permeability (WVP) decreased significantly.					
34999048	0	71	theme	organic	60:66	arg1	frameworks					68:77	covalent organic frameworks	51:77	covalent organic frameworks	51:77	High antibacterial activity of chitosan films with covalent organic frameworks immobilized silver nanoparticles.					
34999048	6	72	dep	showed	1164:1169	arg1	only					1110:1113	only	1110:1113	only	1110:1113	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
34999048	6	73	theme	CS/COFs-AgNPs	1125:1137	arg1	films					1153:1157	the CS/COFs-AgNPs nanocomposite films	1121:1157	the CS/COFs-AgNPs nanocomposite films	1121:1157	Not only that, the CS/COFs-AgNPs nanocomposite films also showed outstanding antibacterial activity and effectively prolonged the storage time of white crucian carp (Carassius auratus).					
35687285	8	0	theme	fluoride-adsorbed	1389:1405	arg1	species					1423:1429	The fluoride-adsorbed F-•••FI-TM-BWCC species	1385:1429	The fluoride-adsorbed F-•••FI-TM-BWCC species	1385:1429	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	6	1	theme	pore	1279:1282	arg1	radius					1284:1289	pore radius	1279:1289	pore radius: 23.64 Å, i.e., mesoporous in nature) to FI-TM-BWCC (216.95 m2/g, avg.	1279:1360	pore radius: 23.64 Å, i.e., mesoporous in nature) to FI-TM-BWCC (216.95 m2/g, avg.					
35687285	8	2	theme	reduced	1442:1448	arg1	area					1458:1461	a reduced surface area	1440:1461	a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake)	1440:1638	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	10	3	theme	natural	2016:2022	arg1	specimens					2036:2044	natural groundwater specimens	2016:2044	natural groundwater specimens	2016:2044	The usefulness of FI-TM-BWCC was diversified through field validation with natural groundwater specimens and proposition of a gravity-fed defluoridation unit.					
35687285	11	4	theme	flow	2104:2107	arg1	rate					2109:2112	The flow rate	2100:2112	The flow rate	2100:2112	The flow rate was documented to be ~11 liters per hour (LPH) by implementing viscous turbulence fluent model.					
35687285	11	4	theme	flow	2104:2107	arg1	liters					2139:2144	~11 liters	2135:2144	~11 liters per hour (LPH)	2135:2159	The flow rate was documented to be ~11 liters per hour (LPH) by implementing viscous turbulence fluent model.					
35687285	9	5	theme	pseudo	1870:1875	arg1	kinetics					1896:1903	pseudo second-order (PSO) kinetics	1870:1903	pseudo second-order (PSO) kinetics	1870:1903	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	1	6	theme	waste	326:330	arg1	terra-firma					341:351	excavation-squander (mine waste)-derived terra-firma	300:351	excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC)	300:391	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	4	7	theme	trace	1009:1013	arg1	amount					1015:1020	a trace amount	1007:1020	a trace amount of anatase	1007:1031	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	7	theme	trace	1009:1013	arg1	anatase					1025:1031	anatase	1025:1031	anatase	1025:1031	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	10	8	with	diversified	1974:1984	arg1	proposition					2050:2060	proposition	2050:2060	proposition of a gravity-fed defluoridation unit	2050:2097	The usefulness of FI-TM-BWCC was diversified through field validation with natural groundwater specimens and proposition of a gravity-fed defluoridation unit.					
35687285	10	8	with	diversified	1974:1984	arg1	specimens					2036:2044	natural groundwater specimens	2016:2044	natural groundwater specimens	2016:2044	The usefulness of FI-TM-BWCC was diversified through field validation with natural groundwater specimens and proposition of a gravity-fed defluoridation unit.					
35687285	8	9	theme	F-	1629:1630	arg1	uptake					1632:1637	F- uptake	1629:1637	F- uptake	1629:1637	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	1	10	theme	adsorbent	212:220	arg1	FI-TM-BWCC					223:232	An inimitable adsorbent "FI-TM-BWCC	198:232	An inimitable adsorbent "FI-TM-BWCC	198:232	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	3	11	theme	removal	938:944	arg1	TM-BWCC					929:935	TM-BWCC	929:935	TM-BWCC	929:935	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	11	theme	removal	938:944	arg1	efficiency					946:955	removal efficiency	938:955	removal efficiency	938:955	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	5	12	theme	BWCC	1255:1258	arg1	area					1247:1250	the BET surface area	1231:1250	the BET surface area of BWCC (~11.92 m2/g, avg	1231:1276	The thermochemical modulation pathway significantly escalated the BET surface area of BWCC (~11.92 m2/g, avg.					
35687285	6	13	dep	mesoporous	1307:1316	arg1	i.e.					1301:1304	i.e.	1301:1304	i.e.	1301:1304	pore radius: 23.64 Å, i.e., mesoporous in nature) to FI-TM-BWCC (216.95 m2/g, avg.					
35687285	3	14	theme	experimental	670:681	arg1	conditions					683:692	identical experimental conditions	660:692	identical experimental conditions	660:692	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	10	15	theme	defluoridation	2079:2092	arg1	unit					2094:2097	a gravity-fed defluoridation unit	2065:2097	a gravity-fed defluoridation unit	2065:2097	The usefulness of FI-TM-BWCC was diversified through field validation with natural groundwater specimens and proposition of a gravity-fed defluoridation unit.					
35687285	3	16	link	cross-linked	811:822	arg1	BWCC					824:827	glutaraldehyde cross-linked BWCC	796:827	glutaraldehyde cross-linked BWCC	796:827	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	16	link	cross-linked	811:822	arg1	adsorbents					756:765	two analogous adsorbents	742:765	two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%)	742:961	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	12	17	theme	experimental	2214:2225	arg1	findings					2227:2234	The experimental findings	2210:2234	The experimental findings	2210:2234	The experimental findings certainly followed the premise conventions of sustainability metrics upholding socio-economic equipoise.					
35687285	9	18	theme	Freundlich	1920:1929	arg1	isotherm					1931:1938	Freundlich isotherm	1920:1938	Freundlich isotherm	1920:1938	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	12	19	theme	premise	2259:2265	arg1	conventions					2267:2277	the premise conventions	2255:2277	the premise conventions of sustainability metrics upholding socio-economic equipoise	2255:2338	The experimental findings certainly followed the premise conventions of sustainability metrics upholding socio-economic equipoise.					
35687285	1	20	theme	present	413:419	arg1	work					421:424	the present work	409:424	the present work	409:424	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	8	21	theme	exchange	1517:1524	arg1	pathway					1526:1532	ion exchange pathway	1513:1532	ion exchange pathway	1513:1532	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	2	22	theme	adsorbate	570:578	arg1	dose					580:583	initial adsorbate dose	562:583	initial adsorbate dose	562:583	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	3	23	theme	F-	699:700	arg1	superior					728:735	superior	728:735	superior	728:735	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	23	theme	F-	699:700	arg1	phenomenon					713:722	the F- scavenging phenomenon	695:722	the F- scavenging phenomenon	695:722	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	24	dep	adsorbents	756:765	arg1	chitosan					783:790	(i) biopolymer chitosan	768:790	(i) biopolymer chitosan	768:790	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	24	dep	adsorbents	756:765	arg1	BWCC					923:926	meta-phase-selective thermally moduled BWCC	884:926	(ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%)	879:961	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	24	dep	adsorbents	756:765	arg1	BWCC					824:827	glutaraldehyde cross-linked BWCC	796:827	glutaraldehyde cross-linked BWCC	796:827	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	24	dep	adsorbents	756:765	arg1	adsorbents					756:765	two analogous adsorbents	742:765	two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%)	742:961	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	25	dep	BWCC	833:836	arg1	%					872:872	74%	870:872	74%	870:872	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	6	26	dep	m2/g	1351:1354	arg1	avg					1357:1359	avg	1357:1359	avg	1357:1359	pore radius: 23.64 Å, i.e., mesoporous in nature) to FI-TM-BWCC (216.95 m2/g, avg.					
35687285	9	27	theme	F1s	1793:1795	arg1	band					1797:1800	a F1s band	1791:1800	a F1s band	1791:1800	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	4	28	theme	41.71	1074:1078	arg1	%					1079:1079	%	1079:1079	%	1079:1079	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	2	29	theme	95	521:522	arg1	%					523:523	%	523:523	%	523:523	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	12	30	theme	sustainability	2282:2295	arg1	metrics					2297:2303	sustainability metrics	2282:2303	sustainability metrics upholding socio-economic equipoise	2282:2338	The experimental findings certainly followed the premise conventions of sustainability metrics upholding socio-economic equipoise.					
35687285	9	31	theme	ion	1746:1748	arg1	CCIE					1760:1763	CCIE	1760:1763	CCIE	1760:1763	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	9	31	theme	ion	1746:1748	arg1	exchange					1750:1757	chemisorption-controlled ion exchange	1721:1757	chemisorption-controlled ion exchange (CCIE) pathway	1721:1772	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	7	32	theme	pore	1362:1365	arg1	radius					1367:1372	pore radius	1362:1372	pore radius	1362:1372	pore radius: 31.41 Å).					
35687285	4	33	theme	prime	1044:1048	arg1	TiO2					1125:1128	3.93% TiO2	1119:1128	3.93% TiO2	1119:1128	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	33	theme	prime	1044:1048	arg1	Al2O3					1081:1085	41.71% Al2O3	1074:1085	41.71% Al2O3	1074:1085	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	33	theme	prime	1044:1048	arg1	Fe2O3					1108:1112	4.25% Fe2O3	1102:1112	4.25% Fe2O3	1102:1112	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	33	theme	prime	1044:1048	arg1	SiO2					1096:1099	49.80 % SiO2	1088:1099	49.80 % SiO2	1088:1099	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	33	theme	prime	1044:1048	arg1	compositions					1060:1071	prime elemental compositions	1044:1071	prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2	1044:1128	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	8	34	theme	surface	1571:1577	arg1	roughness/SA					1579:1590	surface roughness/SA	1571:1590	surface roughness/SA	1571:1590	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	3	35	theme	glutaraldehyde	796:809	arg1	BWCC					824:827	glutaraldehyde cross-linked BWCC	796:827	glutaraldehyde cross-linked BWCC	796:827	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	35	theme	glutaraldehyde	796:809	arg1	adsorbents					756:765	two analogous adsorbents	742:765	two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%)	742:961	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	10	36	theme	FI-TM-BWCC	1959:1968	arg1	usefulness					1945:1954	The usefulness	1941:1954	The usefulness of FI-TM-BWCC	1941:1968	The usefulness of FI-TM-BWCC was diversified through field validation with natural groundwater specimens and proposition of a gravity-fed defluoridation unit.					
35687285	10	36	theme	FI-TM-BWCC	1959:1968	arg1	diversified					1974:1984	diversified	1974:1984	diversified	1974:1984	The usefulness of FI-TM-BWCC was diversified through field validation with natural groundwater specimens and proposition of a gravity-fed defluoridation unit.					
35687285	0	37	theme	fluoride	129:136	arg1	adsorption					138:147	geogenic fluoride adsorption	120:147	geogenic fluoride adsorption	120:147	Strategic optimization of phase-selective thermochemically amended terra-firma originating from excavation-squander for geogenic fluoride adsorption: a combined experimental and in silico approach.					
35687285	3	38	dep	chitosan	783:790	arg1	BWCC					833:836	CG@BWCC	830:836	CG@BWCC	830:836	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	38	dep	chitosan	783:790	arg1	efficiency					858:867	wherein F- removal efficiency	839:867	wherein F- removal efficiency	839:867	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	38	dep	chitosan	783:790	arg1	i					769:769	i	769:769	i	769:769	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	2	39	theme	removal	525:531	arg1	capacity					533:540	95% removal capacity	521:540	95% removal capacity	521:540	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	1	40	dep	clay	375:378	arg1	i.e.					381:384	blackish white china clay, i.e., BWCC	354:390	i.e.	381:384	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	4	41	theme	4.25	1102:1105	arg1	%					1106:1106	%	1106:1106	%	1106:1106	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	8	42	theme	nm	1600:1601	arg1	roughness/SA					1579:1590	surface roughness/SA	1571:1590	surface roughness/SA	1571:1590	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	3	43	theme	CG	830:831	arg1	BWCC					833:836	CG@BWCC	830:836	CG@BWCC	830:836	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	43	theme	CG	830:831	arg1	efficiency					858:867	wherein F- removal efficiency	839:867	wherein F- removal efficiency	839:867	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	10	44	theme	field	1994:1998	arg1	validation					2000:2009	field validation	1994:2009	field validation	1994:2009	The usefulness of FI-TM-BWCC was diversified through field validation with natural groundwater specimens and proposition of a gravity-fed defluoridation unit.					
35687285	9	45	theme	second-order	1877:1888	arg1	kinetics					1896:1903	pseudo second-order (PSO) kinetics	1870:1903	pseudo second-order (PSO) kinetics	1870:1903	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	0	46	theme	Strategic	0:8	arg1	optimization					10:21	Strategic optimization	0:21	Strategic optimization of phase-selective thermochemically	0:57	Strategic optimization of phase-selective thermochemically amended terra-firma originating from excavation-squander for geogenic fluoride adsorption: a combined experimental and in silico approach.					
35687285	3	47	theme	removal	850:856	arg1	BWCC					833:836	CG@BWCC	830:836	CG@BWCC	830:836	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	47	theme	removal	850:856	arg1	efficiency					858:867	wherein F- removal efficiency	839:867	wherein F- removal efficiency	839:867	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	2	48	from	dose	580:583	arg1	Qe					546:547	Qe	546:547	Qe	546:547	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	2	48	from	dose	580:583	arg1	capacity					533:540	95% removal capacity	521:540	95% removal capacity	521:540	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	4	49	theme	49.80	1088:1092	arg1	%					1094:1094	%	1094:1094	%	1094:1094	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	1	50	theme	terra-firma	341:351	arg1	modulation					286:295	meta-phase-selective thermochemical modulation	250:295	meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC)	250:391	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	3	51	theme	wherein	839:845	arg1	BWCC					833:836	CG@BWCC	830:836	CG@BWCC	830:836	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	51	theme	wherein	839:845	arg1	efficiency					858:867	wherein F- removal efficiency	839:867	wherein F- removal efficiency	839:867	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	2	52	theme	excellent	488:496	arg1	efficiency					509:518	an excellent adsorption efficiency	485:518	an excellent adsorption efficiency	485:518	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	9	53	theme	spontaneous	1645:1655	arg1	kJ					1698:1699	ΔG0 = -6.25 kJ	1686:1699	ΔG0 = -6.25 kJ	1686:1699	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	9	53	theme	spontaneous	1645:1655	arg1	interaction					1673:1683	The spontaneous F-•••FI-TM-BWCC interaction	1641:1683	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ)	1641:1700	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	4	54	theme	%	1123:1123	arg1	TiO2					1125:1128	3.93% TiO2	1119:1128	3.93% TiO2	1119:1128	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	54	theme	%	1123:1123	arg1	compositions					1060:1071	prime elemental compositions	1044:1071	prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2	1044:1128	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	5	55	dep	m2/g	1268:1271	arg1	avg					1274:1276	avg	1274:1276	avg	1274:1276	The thermochemical modulation pathway significantly escalated the BET surface area of BWCC (~11.92 m2/g, avg.					
35687285	0	56	theme	thermochemically	42:57	arg1	optimization					10:21	Strategic optimization	0:21	Strategic optimization of phase-selective thermochemically	0:57	Strategic optimization of phase-selective thermochemically amended terra-firma originating from excavation-squander for geogenic fluoride adsorption: a combined experimental and in silico approach.					
35687285	1	57	theme	blackish	354:361	arg1	BWCC					387:390	BWCC	387:390	BWCC	387:390	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	1	57	theme	blackish	354:361	arg1	clay					375:378	blackish white china clay	354:378	blackish white china clay	354:378	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	2	58	dep	capacity	533:540	arg1	mg/L					589:592	10 mg/L	586:592	10 mg/L	586:592	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	2	58	dep	capacity	533:540	arg1	dosage					616:621	adsorbent dosage	606:621	adsorbent dosage	606:621	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	2	58	dep	capacity	533:540	arg1	time					642:645	exposure time	633:645	exposure time	633:645	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	2	58	dep	capacity	533:540	arg1	pH					595:596	pH	595:596	pH	595:596	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	2	58	dep	capacity	533:540	arg1	min					651:653	60 min	648:653	60 min	648:653	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	2	58	dep	capacity	533:540	arg1	7±0.5					599:603	7±0.5	599:603	7±0.5	599:603	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	2	58	dep	capacity	533:540	arg1	mg					629:630	~600 mg	624:630	~600 mg	624:630	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	2	58	dep	capacity	533:540	arg1	mg/g					553:556	99 mg/g	550:556	99 mg/g	550:556	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	0	59	dep	terra-firma	67:77	arg1	approach					188:195	a combined experimental and in silico approach	150:195	approach	188:195	Strategic optimization of phase-selective thermochemically amended terra-firma originating from excavation-squander for geogenic fluoride adsorption: a combined experimental and in silico approach.					
35687285	0	59	dep	terra-firma	67:77	arg1	experimental					161:172	experimental	161:172	experimental	161:172	Strategic optimization of phase-selective thermochemically amended terra-firma originating from excavation-squander for geogenic fluoride adsorption: a combined experimental and in silico approach.					
35687285	1	60	theme	china	369:373	arg1	BWCC					387:390	BWCC	387:390	BWCC	387:390	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	1	60	theme	china	369:373	arg1	clay					375:378	blackish white china clay	354:378	blackish white china clay	354:378	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	8	61	dep	surface	1562:1568	arg1	roughness/SA					1579:1590	surface roughness/SA	1571:1590	surface roughness/SA	1571:1590	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	5	62	theme	surface	1239:1245	arg1	area					1247:1250	the BET surface area	1231:1250	the BET surface area of BWCC (~11.92 m2/g, avg	1231:1276	The thermochemical modulation pathway significantly escalated the BET surface area of BWCC (~11.92 m2/g, avg.					
35687285	9	63	theme	exchange	1750:1757	arg1	pathway					1766:1772	chemisorption-controlled ion exchange (CCIE) pathway	1721:1772	chemisorption-controlled ion exchange (CCIE) pathway	1721:1772	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	1	64	theme	meta-phase-selective	250:269	arg1	modulation					286:295	meta-phase-selective thermochemical modulation	250:295	meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC)	250:391	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	6	65	dep	Å	1298:1298	arg1	m2/g					1351:1354	216.95 m2/g	1344:1354	216.95 m2/g	1344:1354	pore radius: 23.64 Å, i.e., mesoporous in nature) to FI-TM-BWCC (216.95 m2/g, avg.					
35687285	6	65	dep	Å	1298:1298	arg1	mesoporous					1307:1316	mesoporous	1307:1316	mesoporous in nature	1307:1326	pore radius: 23.64 Å, i.e., mesoporous in nature) to FI-TM-BWCC (216.95 m2/g, avg.					
35687285	9	66	theme	F-•••FI-TM-BWCC	1657:1671	arg1	kJ					1698:1699	ΔG0 = -6.25 kJ	1686:1699	ΔG0 = -6.25 kJ	1686:1699	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	9	66	theme	F-•••FI-TM-BWCC	1657:1671	arg1	interaction					1673:1683	The spontaneous F-•••FI-TM-BWCC interaction	1641:1683	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ)	1641:1700	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	9	67	theme	Si-F	1835:1838	arg1	formation					1845:1853	Si-F bond formation	1835:1853	Si-F bond formation	1835:1853	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	5	68	theme	modulation	1188:1197	arg1	pathway					1199:1205	The thermochemical modulation pathway	1169:1205	The thermochemical modulation pathway	1169:1205	The thermochemical modulation pathway significantly escalated the BET surface area of BWCC (~11.92 m2/g, avg.					
35687285	3	69	dep	BWCC	923:926	arg1	TM-BWCC					929:935	TM-BWCC	929:935	TM-BWCC	929:935	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	69	dep	BWCC	923:926	arg1	efficiency					946:955	removal efficiency	938:955	removal efficiency	938:955	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	69	dep	BWCC	923:926	arg1	ii					880:881	ii	880:881	ii	880:881	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	4	70	theme	XRF	1155:1157	arg1	analyses					1159:1166	XRF analyses	1155:1166	XRF analyses	1155:1166	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	1	71	link	-derived	332:339	arg1	terra-firma					341:351	excavation-squander (mine waste)-derived terra-firma	300:351	excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC)	300:391	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	3	72	dep	TM-BWCC	929:935	arg1	%					960:960	75%	958:960	75%	958:960	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	1	73	theme	excavation-squander	300:318	arg1	terra-firma					341:351	excavation-squander (mine waste)-derived terra-firma	300:351	excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC)	300:391	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	10	74	theme	groundwater	2024:2034	arg1	specimens					2036:2044	natural groundwater specimens	2016:2044	natural groundwater specimens	2016:2044	The usefulness of FI-TM-BWCC was diversified through field validation with natural groundwater specimens and proposition of a gravity-fed defluoridation unit.					
35687285	3	75	theme	moduled	915:921	arg1	BWCC					923:926	meta-phase-selective thermally moduled BWCC	884:926	(ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%)	879:961	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	75	theme	moduled	915:921	arg1	adsorbents					756:765	two analogous adsorbents	742:765	two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%)	742:961	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	12	76	theme	metrics	2297:2303	arg1	conventions					2267:2277	the premise conventions	2255:2277	the premise conventions of sustainability metrics upholding socio-economic equipoise	2255:2338	The experimental findings certainly followed the premise conventions of sustainability metrics upholding socio-economic equipoise.					
35687285	1	77	theme	adsorption	444:453	arg1	purpose					455:461	fluoride (F-) adsorption purpose	430:461	fluoride (F-) adsorption purpose	430:461	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	11	78	theme	turbulence	2185:2194	arg1	model					2203:2207	viscous turbulence fluent model	2177:2207	viscous turbulence fluent model	2177:2207	The flow rate was documented to be ~11 liters per hour (LPH) by implementing viscous turbulence fluent model.					
35687285	1	79	theme	mine	321:324	arg1	terra-firma					341:351	excavation-squander (mine waste)-derived terra-firma	300:351	excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC)	300:391	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	3	80	theme	meta-phase-selective	884:903	arg1	BWCC					923:926	meta-phase-selective thermally moduled BWCC	884:926	(ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%)	879:961	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	80	theme	meta-phase-selective	884:903	arg1	adsorbents					756:765	two analogous adsorbents	742:765	two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%)	742:961	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	8	81	theme	F-•••FI-TM-BWCC	1407:1421	arg1	species					1423:1429	The fluoride-adsorbed F-•••FI-TM-BWCC species	1385:1429	The fluoride-adsorbed F-•••FI-TM-BWCC species	1385:1429	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	3	82	theme	identical	660:668	arg1	conditions					683:692	identical experimental conditions	660:692	identical experimental conditions	660:692	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	1	83	theme	inimitable	201:210	arg1	FI-TM-BWCC					223:232	An inimitable adsorbent "FI-TM-BWCC	198:232	An inimitable adsorbent "FI-TM-BWCC	198:232	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	4	84	theme	anatase	1025:1031	arg1	amount					1015:1020	a trace amount	1007:1020	a trace amount of anatase	1007:1031	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	84	theme	anatase	1025:1031	arg1	kaolinite					993:1001	kaolinite	993:1001	kaolinite	993:1001	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	84	theme	anatase	1025:1031	arg1	anatase					1025:1031	anatase	1025:1031	anatase	1025:1031	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	11	85	theme	viscous	2177:2183	arg1	model					2203:2207	viscous turbulence fluent model	2177:2207	viscous turbulence fluent model	2177:2207	The flow rate was documented to be ~11 liters per hour (LPH) by implementing viscous turbulence fluent model.					
35687285	9	86	theme	PSO	1891:1893	arg1	kinetics					1896:1903	pseudo second-order (PSO) kinetics	1870:1903	pseudo second-order (PSO) kinetics	1870:1903	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	10	87	theme	unit	2094:2097	arg1	proposition					2050:2060	proposition	2050:2060	proposition of a gravity-fed defluoridation unit	2050:2097	The usefulness of FI-TM-BWCC was diversified through field validation with natural groundwater specimens and proposition of a gravity-fed defluoridation unit.					
35687285	10	87	theme	unit	2094:2097	arg1	specimens					2036:2044	natural groundwater specimens	2016:2044	natural groundwater specimens	2016:2044	The usefulness of FI-TM-BWCC was diversified through field validation with natural groundwater specimens and proposition of a gravity-fed defluoridation unit.					
35687285	1	88	dep	adsorption	444:453	arg1	fluoride					430:437	fluoride	430:437	fluoride	430:437	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	1	88	dep	adsorption	444:453	arg1	F-					440:441	F-	440:441	F-	440:441	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	9	89	theme	=	1690:1690	arg1	kJ					1698:1699	ΔG0 = -6.25 kJ	1686:1699	ΔG0 = -6.25 kJ	1686:1699	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	9	89	theme	=	1690:1690	arg1	interaction					1673:1683	The spontaneous F-•••FI-TM-BWCC interaction	1641:1683	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ)	1641:1700	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	0	90	theme	in	178:179	arg1	approach					188:195	a combined experimental and in silico approach	150:195	approach	188:195	Strategic optimization of phase-selective thermochemically amended terra-firma originating from excavation-squander for geogenic fluoride adsorption: a combined experimental and in silico approach.					
35687285	5	91	dep	area	1247:1250	arg1	m2/g					1268:1271	~11.92 m2/g	1261:1271	~11.92 m2/g	1261:1271	The thermochemical modulation pathway significantly escalated the BET surface area of BWCC (~11.92 m2/g, avg.					
35687285	4	92	dep	compositions	1060:1071	arg1	TiO2					1125:1128	3.93% TiO2	1119:1128	3.93% TiO2	1119:1128	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	92	dep	compositions	1060:1071	arg1	Al2O3					1081:1085	41.71% Al2O3	1074:1085	41.71% Al2O3	1074:1085	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	92	dep	compositions	1060:1071	arg1	compositions					1060:1071	prime elemental compositions	1044:1071	prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2	1044:1128	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	92	dep	compositions	1060:1071	arg1	Fe2O3					1108:1112	4.25% Fe2O3	1102:1112	4.25% Fe2O3	1102:1112	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	92	dep	compositions	1060:1071	arg1	SiO2					1096:1099	49.80 % SiO2	1088:1099	49.80 % SiO2	1088:1099	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	12	93	theme	socio-economic	2315:2328	arg1	equipoise					2330:2338	socio-economic equipoise	2315:2338	socio-economic equipoise	2315:2338	The experimental findings certainly followed the premise conventions of sustainability metrics upholding socio-economic equipoise.					
35687285	10	94	theme	gravity-fed	2067:2077	arg1	unit					2094:2097	a gravity-fed defluoridation unit	2065:2097	a gravity-fed defluoridation unit	2065:2097	The usefulness of FI-TM-BWCC was diversified through field validation with natural groundwater specimens and proposition of a gravity-fed defluoridation unit.					
35687285	8	95	theme	ion	1513:1515	arg1	pathway					1526:1532	ion exchange pathway	1513:1532	ion exchange pathway	1513:1532	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	7	96	theme	31.41	1375:1379	arg1	Å					1381:1381	31.41 Å)	1375:1382	31.41 Å)	1375:1382	pore radius: 31.41 Å).					
35687285	2	97	theme	initial	562:568	arg1	dose					580:583	initial adsorbate dose	562:583	initial adsorbate dose	562:583	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	4	98	theme	%	1079:1079	arg1	Al2O3					1081:1085	41.71% Al2O3	1074:1085	41.71% Al2O3	1074:1085	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	98	theme	%	1079:1079	arg1	compositions					1060:1071	prime elemental compositions	1044:1071	prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2	1044:1128	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	3	99	theme	scavenging	702:711	arg1	superior					728:735	superior	728:735	superior	728:735	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	99	theme	scavenging	702:711	arg1	phenomenon					713:722	the F- scavenging phenomenon	695:722	the F- scavenging phenomenon	695:722	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	8	100	theme	pathway	1526:1532	arg1	light					1504:1508	the light	1500:1508	the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake)	1500:1638	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	9	101	theme	chemisorption-controlled	1721:1744	arg1	CCIE					1760:1763	CCIE	1760:1763	CCIE	1760:1763	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	9	101	theme	chemisorption-controlled	1721:1744	arg1	exchange					1750:1757	chemisorption-controlled ion exchange	1721:1757	chemisorption-controlled ion exchange (CCIE) pathway	1721:1772	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	9	102	theme	ΔG0	1686:1688	arg1	kJ					1698:1699	ΔG0 = -6.25 kJ	1686:1699	ΔG0 = -6.25 kJ	1686:1699	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	9	102	theme	ΔG0	1686:1688	arg1	interaction					1673:1683	The spontaneous F-•••FI-TM-BWCC interaction	1641:1683	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ)	1641:1700	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	3	103	theme	analogous	746:754	arg1	chitosan					783:790	(i) biopolymer chitosan	768:790	(i) biopolymer chitosan	768:790	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	103	theme	analogous	746:754	arg1	BWCC					923:926	meta-phase-selective thermally moduled BWCC	884:926	(ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%)	879:961	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	103	theme	analogous	746:754	arg1	BWCC					824:827	glutaraldehyde cross-linked BWCC	796:827	glutaraldehyde cross-linked BWCC	796:827	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	103	theme	analogous	746:754	arg1	adsorbents					756:765	two analogous adsorbents	742:765	two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%)	742:961	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	0	104	dep	in	178:179	arg1	silico					181:186	silico	181:186	silico	181:186	Strategic optimization of phase-selective thermochemically amended terra-firma originating from excavation-squander for geogenic fluoride adsorption: a combined experimental and in silico approach.					
35687285	3	105	theme	biopolymer	772:781	arg1	chitosan					783:790	(i) biopolymer chitosan	768:790	(i) biopolymer chitosan	768:790	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	105	theme	biopolymer	772:781	arg1	adsorbents					756:765	two analogous adsorbents	742:765	two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%)	742:961	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	1	106	dep	terra-firma	341:351	arg1	BWCC					387:390	BWCC	387:390	BWCC	387:390	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	1	106	dep	terra-firma	341:351	arg1	clay					375:378	blackish white china clay	354:378	blackish white china clay	354:378	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	4	107	theme	elemental	1050:1058	arg1	TiO2					1125:1128	3.93% TiO2	1119:1128	3.93% TiO2	1119:1128	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	107	theme	elemental	1050:1058	arg1	Al2O3					1081:1085	41.71% Al2O3	1074:1085	41.71% Al2O3	1074:1085	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	107	theme	elemental	1050:1058	arg1	Fe2O3					1108:1112	4.25% Fe2O3	1102:1112	4.25% Fe2O3	1102:1112	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	107	theme	elemental	1050:1058	arg1	SiO2					1096:1099	49.80 % SiO2	1088:1099	49.80 % SiO2	1088:1099	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	107	theme	elemental	1050:1058	arg1	compositions					1060:1071	prime elemental compositions	1044:1071	prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2	1044:1128	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	9	108	from	eV	1811:1812	arg1	appearance					1777:1786	appearance	1777:1786	appearance of a F1s band at 685.5 eV	1777:1812	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	0	109	theme	geogenic	120:127	arg1	adsorption					138:147	geogenic fluoride adsorption	120:147	geogenic fluoride adsorption	120:147	Strategic optimization of phase-selective thermochemically amended terra-firma originating from excavation-squander for geogenic fluoride adsorption: a combined experimental and in silico approach.					
35687285	2	110	dep	portrayed	475:483	arg1	Qe					546:547	Qe	546:547	Qe	546:547	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	2	110	dep	portrayed	475:483	arg1	capacity					533:540	95% removal capacity	521:540	95% removal capacity	521:540	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	8	111	theme	non-uniform	1550:1560	arg1	surface					1562:1568	FI-TM-BWCC's non-uniform surface	1537:1568	FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake)	1537:1638	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	2	112	theme	adsorption	498:507	arg1	efficiency					509:518	an excellent adsorption efficiency	485:518	an excellent adsorption efficiency	485:518	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	2	113	theme	adsorbent	606:614	arg1	dosage					616:621	adsorbent dosage	606:621	adsorbent dosage	606:621	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	3	114	theme	cross-linked	811:822	arg1	BWCC					824:827	glutaraldehyde cross-linked BWCC	796:827	glutaraldehyde cross-linked BWCC	796:827	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	114	theme	cross-linked	811:822	arg1	adsorbents					756:765	two analogous adsorbents	742:765	two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%)	742:961	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	4	115	theme	%	1106:1106	arg1	Fe2O3					1108:1112	4.25% Fe2O3	1102:1112	4.25% Fe2O3	1102:1112	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	115	theme	%	1106:1106	arg1	compositions					1060:1071	prime elemental compositions	1044:1071	prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2	1044:1128	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	7	116	dep	Å	1381:1381	arg1	radius					1367:1372	pore radius	1362:1372	pore radius	1362:1372	pore radius: 31.41 Å).					
35687285	2	117	theme	%	523:523	arg1	capacity					533:540	95% removal capacity	521:540	95% removal capacity	521:540	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	3	118	from	conditions	683:692	arg1	superior					728:735	superior	728:735	superior	728:735	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	118	from	conditions	683:692	arg1	phenomenon					713:722	the F- scavenging phenomenon	695:722	the F- scavenging phenomenon	695:722	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	2	119	theme	exposure	633:640	arg1	time					642:645	exposure time	633:645	exposure time	633:645	FI-TM-BWCC portrayed an excellent adsorption efficiency (95% removal capacity and Qe: 99 mg/g, at initial adsorbate dose: 10 mg/L, pH: 7±0.5, adsorbent dosage: ~600 mg, exposure time: 60 min).					
35687285	6	120	from	mesoporous	1307:1316	arg1	nature					1321:1326	nature	1321:1326	nature	1321:1326	pore radius: 23.64 Å, i.e., mesoporous in nature) to FI-TM-BWCC (216.95 m2/g, avg.					
35687285	3	121	theme	@	832:832	arg1	BWCC					833:836	CG@BWCC	830:836	CG@BWCC	830:836	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	121	theme	@	832:832	arg1	efficiency					858:867	wherein F- removal efficiency	839:867	wherein F- removal efficiency	839:867	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	4	122	theme	%	1094:1094	arg1	compositions					1060:1071	prime elemental compositions	1044:1071	prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2	1044:1128	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	4	122	theme	%	1094:1094	arg1	SiO2					1096:1099	49.80 % SiO2	1088:1099	49.80 % SiO2	1088:1099	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	0	123	theme	phase-selective	26:40	arg1	thermochemically					42:57	phase-selective thermochemically	26:57	phase-selective thermochemically	26:57	Strategic optimization of phase-selective thermochemically amended terra-firma originating from excavation-squander for geogenic fluoride adsorption: a combined experimental and in silico approach.					
35687285	1	124	theme	-derived	332:339	arg1	terra-firma					341:351	excavation-squander (mine waste)-derived terra-firma	300:351	excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC)	300:391	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	9	125	theme	band	1797:1800	arg1	appearance					1777:1786	appearance	1777:1786	appearance of a F1s band at 685.5 eV	1777:1812	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
35687285	3	126	theme	F-	847:848	arg1	BWCC					833:836	CG@BWCC	830:836	CG@BWCC	830:836	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	3	126	theme	F-	847:848	arg1	efficiency					858:867	wherein F- removal efficiency	839:867	wherein F- removal efficiency	839:867	At identical experimental conditions, the F- scavenging phenomenon was superior than two analogous adsorbents: (i) biopolymer chitosan and glutaraldehyde cross-linked BWCC (CG@BWCC, wherein F- removal efficiency: 74%) and (ii) meta-phase-selective thermally moduled BWCC (TM-BWCC, removal efficiency: 75%).					
35687285	1	127	theme	white	363:367	arg1	BWCC					387:390	BWCC	387:390	BWCC	387:390	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	1	127	theme	white	363:367	arg1	clay					375:378	blackish white china clay	354:378	blackish white china clay	354:378	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	8	128	theme	m2/g	1471:1474	arg1	area					1458:1461	a reduced surface area	1440:1461	a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake)	1440:1638	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	5	129	theme	BET	1235:1237	arg1	area					1247:1250	the BET surface area	1231:1250	the BET surface area of BWCC (~11.92 m2/g, avg	1231:1276	The thermochemical modulation pathway significantly escalated the BET surface area of BWCC (~11.92 m2/g, avg.					
35687285	6	130	dep	radius	1284:1289	arg1	Å					1298:1298	23.64 Å	1292:1298	pore radius: 23.64 Å, i.e., mesoporous in nature) to FI-TM-BWCC (216.95 m2/g, avg.	1279:1360	pore radius: 23.64 Å, i.e., mesoporous in nature) to FI-TM-BWCC (216.95 m2/g, avg.					
35687285	4	131	theme	3.93	1119:1122	arg1	%					1123:1123	%	1123:1123	%	1123:1123	BWCC predominantly comprises kaolinite and a trace amount of anatase along with prime elemental compositions: 41.71% Al2O3, 49.80 % SiO2, 4.25% Fe2O3, and 3.93% TiO2, as revealed by PXRD and XRF analyses.					
35687285	5	132	theme	thermochemical	1173:1186	arg1	pathway					1199:1205	The thermochemical modulation pathway	1169:1205	The thermochemical modulation pathway	1169:1205	The thermochemical modulation pathway significantly escalated the BET surface area of BWCC (~11.92 m2/g, avg.					
35687285	8	133	theme	surface	1450:1456	arg1	area					1458:1461	a reduced surface area	1440:1461	a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake)	1440:1638	The fluoride-adsorbed F-•••FI-TM-BWCC species revealed a reduced surface area of 21.5 m2/g, which was explained in the light of ion exchange pathway on FI-TM-BWCC's non-uniform surface (surface roughness/SA of 1597 nm, reduced to 1179 nm after F- uptake).					
35687285	1	134	theme	thermochemical	271:284	arg1	modulation					286:295	meta-phase-selective thermochemical modulation	250:295	meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC)	250:391	An inimitable adsorbent "FI-TM-BWCC," emanated from meta-phase-selective thermochemical modulation of excavation-squander (mine waste)-derived terra-firma (blackish white china clay, i.e., BWCC), is explored in the present work for fluoride (F-) adsorption purpose.					
35687285	11	135	theme	fluent	2196:2201	arg1	model					2203:2207	viscous turbulence fluent model	2177:2207	viscous turbulence fluent model	2177:2207	The flow rate was documented to be ~11 liters per hour (LPH) by implementing viscous turbulence fluent model.					
35687285	9	136	theme	bond	1840:1843	arg1	formation					1845:1853	Si-F bond formation	1835:1853	Si-F bond formation	1835:1853	The spontaneous F-•••FI-TM-BWCC interaction (ΔG0 = -6.25 kJ) occurred following chemisorption-controlled ion exchange (CCIE) pathway as appearance of a F1s band at 685.5 eV was rationalized for Si-F bond formation; corroborating pseudo second-order (PSO) kinetics and resembling Freundlich isotherm.					
36144631	0	0	theme	Specific	116:123	arg1	Antigen					125:131	Prostate Specific Antigen	107:131	Prostate Specific Antigen	107:131	Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.					
36144631	4	1	theme	CS-rGO	999:1004	arg1	film					1016:1019	the CS-rGO composite film	995:1019	the CS-rGO composite film	995:1019	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	1	2	theme	antigen	199:205	arg1	determination					164:176	A facile and highly sensitive determination	134:176	A facile and highly sensitive determination of prostate-specific antigen (PSA)	134:211	A facile and highly sensitive determination of prostate-specific antigen (PSA) is of great significance for the early diagnosis, monitoring and prognosis of prostate cancer.					
36144631	6	3	theme	detection	1437:1445	arg1	limit					1428:1432	a low limit	1422:1432	a low limit of detection of 0.8 pg mL-1	1422:1460	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	7	4	theme	good	1529:1532	arg1	stability					1542:1550	good storage stability	1529:1550	good storage stability	1529:1550	This sensor also exhibited high selectivity, reproducibility, and good storage stability.					
36144631	0	5	theme	Prostate	107:114	arg1	Antigen					125:131	Prostate Specific Antigen	107:131	Prostate Specific Antigen	107:131	Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.					
36144631	4	6	theme	antibodies	875:884	arg1	immobilization					845:858	the immobilization	841:858	the immobilization of recognition antibodies and rGO	841:892	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	5	7	theme	polyclonal	1119:1128	arg1	Ab					1149:1150	Ab	1149:1150	Ab	1149:1150	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	5	7	theme	polyclonal	1119:1128	arg1	antibody					1139:1146	specific polyclonal anti-PSA antibody	1110:1146	a specific polyclonal anti-PSA antibody (Ab)	1108:1151	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	5	8	theme	cross-linking	1226:1238	arg1	method					1240:1245	a facile glutaraldehyde (GA) cross-linking method	1197:1245	a facile glutaraldehyde (GA) cross-linking method	1197:1245	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	4	9	theme	electronic	954:963	arg1	conductivity					965:976	the electronic conductivity	950:976	the electronic conductivity	950:976	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	5	10	theme	amino-enriched	1160:1173	arg1	surface					1185:1191	the amino-enriched electrode surface	1156:1191	the amino-enriched electrode surface	1156:1191	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	6	11	with	mL-1	1412:1415	arg1	limit					1428:1432	a low limit	1422:1432	a low limit of detection of 0.8 pg mL-1	1422:1460	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	4	12	theme	patterned	769:777	arg1	ITO					779:781	the patterned ITO	765:781	the patterned ITO	765:781	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	5	13	theme	bovine	1281:1286	arg1	albumin					1294:1300	bovine serum albumin	1281:1300	bovine serum albumin	1281:1300	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	6	14	theme	PSA	1377:1379	arg1	detection					1364:1372	the detection	1360:1372	the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1	1360:1460	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	5	15	theme	anti-PSA	1130:1137	arg1	Ab					1149:1150	Ab	1149:1150	Ab	1149:1150	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	5	15	theme	anti-PSA	1130:1137	arg1	antibody					1139:1146	specific polyclonal anti-PSA antibody	1110:1146	a specific polyclonal anti-PSA antibody (Ab)	1108:1151	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	4	16	theme	rGO	890:892	arg1	immobilization					845:858	the immobilization	841:858	the immobilization of recognition antibodies and rGO	841:892	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	1	17	theme	prostate	291:298	arg1	cancer					300:305	prostate cancer	291:305	prostate cancer	291:305	A facile and highly sensitive determination of prostate-specific antigen (PSA) is of great significance for the early diagnosis, monitoring and prognosis of prostate cancer.					
36144631	5	18	theme	antibody	1139:1146	arg1	immobilization					1090:1103	covalent immobilization	1081:1103	covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface	1081:1191	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	8	19	theme	human	1652:1656	arg1	samples					1664:1670	spiked human serum samples	1645:1670	spiked human serum samples	1645:1670	The application of the prepared immunosensor was successfully validated by measuring PSA in spiked human serum samples.					
36144631	3	20	theme	graphene	566:573	arg1	rGO					582:584	rGO	582:584	rGO	582:584	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	3	20	theme	graphene	566:573	arg1	oxide					575:579	graphene oxide	566:579	reduced graphene oxide (rGO) nanocomposite (CS-rGO)	558:608	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	8	21	theme	spiked	1645:1650	arg1	samples					1664:1670	spiked human serum samples	1645:1670	spiked human serum samples	1645:1670	The application of the prepared immunosensor was successfully validated by measuring PSA in spiked human serum samples.					
36144631	2	22	theme	tin	449:451	arg1	ITO					460:462	ITO	460:462	ITO	460:462	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	2	22	theme	tin	449:451	arg1	oxide					453:457	indium tin oxide	442:457	chitosan-graphene-modified indium tin oxide (ITO) electrode	415:473	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	2	23	theme	sensitive	490:498	arg1	determination					513:525	sensitive amperometric determination	490:525	sensitive amperometric determination of PSA	490:532	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	3	24	theme	biofunctionalizing	711:728	arg1	agent					730:734	a dispersant and biofunctionalizing agent	694:734	a dispersant and biofunctionalizing agent	694:734	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	3	24	theme	biofunctionalizing	711:728	arg1	CS					688:689	CS	688:689	CS	688:689	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	4	25	theme	film	1016:1019	arg1	conductivity					965:976	the electronic conductivity	950:976	the electronic conductivity	950:976	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	4	25	theme	film	1016:1019	arg1	stability					982:990	stability	982:990	stability	982:990	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	3	26	theme	oxide	575:579	arg1	nanocomposite					587:599	reduced graphene oxide (rGO) nanocomposite	558:599	reduced graphene oxide (rGO) nanocomposite (CS-rGO)	558:608	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	3	26	theme	oxide	575:579	arg1	CS-rGO					602:607	CS-rGO	602:607	CS-rGO	602:607	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	6	27	theme	ng	1409:1410	arg1	mL-1					1412:1415	1 to 5 ng mL-1	1402:1415	1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1	1402:1460	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	2	28	theme	indium	442:447	arg1	ITO					460:462	ITO	460:462	ITO	460:462	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	2	28	theme	indium	442:447	arg1	oxide					453:457	indium tin oxide	442:457	chitosan-graphene-modified indium tin oxide (ITO) electrode	415:473	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	0	29	theme	Amperometric	11:22	arg1	Immunosensor					35:46	Disposable Amperometric Label-Free Immunosensor	0:46	Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.	0:132	Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.					
36144631	2	30	theme	PSA	530:532	arg1	determination					513:525	sensitive amperometric determination	490:525	sensitive amperometric determination of PSA	490:532	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	4	31	theme	recognition	863:873	arg1	antibodies					875:884	recognition antibodies	863:884	recognition antibodies	863:884	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	5	32	theme	facile	1199:1204	arg1	method					1240:1245	a facile glutaraldehyde (GA) cross-linking method	1197:1245	a facile glutaraldehyde (GA) cross-linking method	1197:1245	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	2	33	theme	disposable	324:333	arg1	platform					380:387	a disposable and label-free electrochemical immunosensing platform	322:387	a disposable and label-free electrochemical immunosensing platform	322:387	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	6	34	from	detection	1364:1372	arg1	range					1391:1395	a wide range	1384:1395	a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1	1384:1460	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	6	34	from	detection	1364:1372	arg1	mL-1					1412:1415	1 to 5 ng mL-1	1402:1415	1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1	1402:1460	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	2	35	theme	chitosan-graphene-modified	415:440	arg1	electrode					465:473	chitosan-graphene-modified indium tin oxide (ITO) electrode	415:473	chitosan-graphene-modified indium tin oxide (ITO) electrode	415:473	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	0	36	theme	Disposable	0:9	arg1	Immunosensor					35:46	Disposable Amperometric Label-Free Immunosensor	0:46	Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.	0:132	Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.					
36144631	1	37	theme	cancer	300:305	arg1	prognosis					278:286	prognosis	278:286	prognosis	278:286	A facile and highly sensitive determination of prostate-specific antigen (PSA) is of great significance for the early diagnosis, monitoring and prognosis of prostate cancer.					
36144631	1	37	theme	cancer	300:305	arg1	monitoring					263:272	monitoring	263:272	monitoring	263:272	A facile and highly sensitive determination of prostate-specific antigen (PSA) is of great significance for the early diagnosis, monitoring and prognosis of prostate cancer.					
36144631	1	37	theme	cancer	300:305	arg1	diagnosis					252:260	the early diagnosis	242:260	the early diagnosis	242:260	A facile and highly sensitive determination of prostate-specific antigen (PSA) is of great significance for the early diagnosis, monitoring and prognosis of prostate cancer.					
36144631	8	38	theme	immunosensor	1585:1596	arg1	application					1557:1567	The application	1553:1567	The application of the prepared immunosensor	1553:1596	The application of the prepared immunosensor was successfully validated by measuring PSA in spiked human serum samples.					
36144631	5	39	theme	non-specific	1315:1326	arg1	sites					1328:1332	the non-specific sites	1311:1332	the non-specific sites	1311:1332	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	5	40	theme	affinity-based	1026:1039	arg1	interface					1052:1060	The affinity-based biosensing interface	1022:1060	The affinity-based biosensing interface	1022:1060	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	1	41	theme	great	219:223	arg1	significance					225:236	great significance	219:236	great significance for the early diagnosis, monitoring and prognosis of prostate cancer	219:305	A facile and highly sensitive determination of prostate-specific antigen (PSA) is of great significance for the early diagnosis, monitoring and prognosis of prostate cancer.					
36144631	0	42	theme	Label-Free	24:33	arg1	Immunosensor					35:46	Disposable Amperometric Label-Free Immunosensor	0:46	Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.	0:132	Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.					
36144631	7	43	theme	storage	1534:1540	arg1	stability					1542:1550	good storage stability	1529:1550	good storage stability	1529:1550	This sensor also exhibited high selectivity, reproducibility, and good storage stability.					
36144631	3	44	theme	oxide	671:675	arg1	reduction					649:657	the chemical reduction	636:657	the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent	636:734	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	1	45	theme	facile	136:141	arg1	determination					164:176	A facile and highly sensitive determination	134:176	A facile and highly sensitive determination of prostate-specific antigen (PSA)	134:211	A facile and highly sensitive determination of prostate-specific antigen (PSA) is of great significance for the early diagnosis, monitoring and prognosis of prostate cancer.					
36144631	0	46	theme	Chitosan-Graphene-Modified	51:76	arg1	Electrodes					92:101	Chitosan-Graphene-Modified Patterned ITO Electrodes	51:101	Chitosan-Graphene-Modified Patterned ITO Electrodes	51:101	Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.					
36144631	6	47	from	mL-1	1412:1415	arg1	detection					1364:1372	the detection	1360:1372	the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1	1360:1460	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	6	47	from	mL-1	1412:1415	arg1	range					1391:1395	a wide range	1384:1395	a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1	1384:1460	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	4	48	theme	reactive	821:828	arg1	element					918:924	a transduction element	903:924	a transduction element	903:924	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	4	48	theme	reactive	821:828	arg1	groups					830:835	reactive groups	821:835	reactive groups for the immobilization of recognition antibodies and rGO	821:892	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	4	49	theme	composite	1006:1014	arg1	film					1016:1019	the CS-rGO composite film	995:1019	the CS-rGO composite film	995:1019	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	5	50	theme	electrode	1175:1183	arg1	surface					1185:1191	the amino-enriched electrode surface	1156:1191	the amino-enriched electrode surface	1156:1191	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	6	51	dep	5	1407:1407	arg1	to					1404:1405	to	1404:1405	to	1404:1405	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	5	52	theme	specific	1110:1117	arg1	Ab					1149:1150	Ab	1149:1150	Ab	1149:1150	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	5	52	theme	specific	1110:1117	arg1	antibody					1139:1146	specific polyclonal anti-PSA antibody	1110:1146	a specific polyclonal anti-PSA antibody (Ab)	1108:1151	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	5	53	theme	biosensing	1041:1050	arg1	interface					1052:1060	The affinity-based biosensing interface	1022:1060	The affinity-based biosensing interface	1022:1060	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	2	54	theme	oxide	453:457	arg1	electrode					465:473	chitosan-graphene-modified indium tin oxide (ITO) electrode	415:473	chitosan-graphene-modified indium tin oxide (ITO) electrode	415:473	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	0	55	theme	ITO	88:90	arg1	Electrodes					92:101	Chitosan-Graphene-Modified Patterned ITO Electrodes	51:101	Chitosan-Graphene-Modified Patterned ITO Electrodes	51:101	Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.					
36144631	5	56	from	immobilization	1090:1103	arg1	surface					1185:1191	the amino-enriched electrode surface	1156:1191	the amino-enriched electrode surface	1156:1191	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	3	57	theme	graphene	662:669	arg1	oxide					671:675	graphene oxide	662:675	graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent	662:734	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	2	58	theme	immunosensing	366:378	arg1	platform					380:387	a disposable and label-free electrochemical immunosensing platform	322:387	a disposable and label-free electrochemical immunosensing platform	322:387	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	0	59	theme	Patterned	78:86	arg1	Electrodes					92:101	Chitosan-Graphene-Modified Patterned ITO Electrodes	51:101	Chitosan-Graphene-Modified Patterned ITO Electrodes	51:101	Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.					
36144631	3	60	theme	dispersant	696:705	arg1	agent					730:734	a dispersant and biofunctionalizing agent	694:734	a dispersant and biofunctionalizing agent	694:734	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	3	60	theme	dispersant	696:705	arg1	CS					688:689	CS	688:689	CS	688:689	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	2	61	theme	electrochemical	350:364	arg1	platform					380:387	a disposable and label-free electrochemical immunosensing platform	322:387	a disposable and label-free electrochemical immunosensing platform	322:387	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	5	62	theme	serum	1288:1292	arg1	albumin					1294:1300	bovine serum albumin	1281:1300	bovine serum albumin	1281:1300	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	6	63	theme	wide	1386:1389	arg1	range					1391:1395	a wide range	1384:1395	a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1	1384:1460	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	3	64	theme	chemical	640:647	arg1	reduction					649:657	the chemical reduction	636:657	the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent	636:734	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	6	65	theme	low	1424:1426	arg1	limit					1428:1432	a low limit	1422:1432	a low limit of detection of 0.8 pg mL-1	1422:1460	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	1	66	theme	sensitive	154:162	arg1	determination					164:176	A facile and highly sensitive determination	134:176	A facile and highly sensitive determination of prostate-specific antigen (PSA)	134:211	A facile and highly sensitive determination of prostate-specific antigen (PSA) is of great significance for the early diagnosis, monitoring and prognosis of prostate cancer.					
36144631	5	67	theme	albumin	1294:1300	arg1	use					1274:1276	the use	1270:1276	the use of bovine serum albumin to block the non-specific sites	1270:1332	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	1	68	theme	early	246:250	arg1	diagnosis					252:260	the early diagnosis	242:260	the early diagnosis	242:260	A facile and highly sensitive determination of prostate-specific antigen (PSA) is of great significance for the early diagnosis, monitoring and prognosis of prostate cancer.					
36144631	4	69	theme	transduction	905:916	arg1	element					918:924	a transduction element	903:924	a transduction element	903:924	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	4	69	theme	transduction	905:916	arg1	groups					830:835	reactive groups	821:835	reactive groups for the immobilization of recognition antibodies and rGO	821:892	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	5	70	theme	covalent	1081:1088	arg1	immobilization					1090:1103	covalent immobilization	1081:1103	covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface	1081:1191	The affinity-based biosensing interface was constructed by covalent immobilization of a specific polyclonal anti-PSA antibody (Ab) on the amino-enriched electrode surface via a facile glutaraldehyde (GA) cross-linking method, which was followed by the use of bovine serum albumin to block the non-specific sites.					
36144631	3	71	theme	reduced	558:564	arg1	nanocomposite					587:599	reduced graphene oxide (rGO) nanocomposite	558:599	reduced graphene oxide (rGO) nanocomposite (CS-rGO)	558:608	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	3	71	theme	reduced	558:564	arg1	CS-rGO					602:607	CS-rGO	602:607	CS-rGO	602:607	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	6	72	theme	pg	1454:1455	arg1	mL-1					1457:1460	0.8 pg mL-1	1450:1460	0.8 pg mL-1	1450:1460	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	2	73	theme	label-free	339:348	arg1	platform					380:387	a disposable and label-free electrochemical immunosensing platform	322:387	a disposable and label-free electrochemical immunosensing platform	322:387	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	6	74	theme	mL-1	1457:1460	arg1	detection					1437:1445	detection	1437:1445	detection of 0.8 pg mL-1	1437:1460	The immunosensor allowed the detection of PSA in a wide range from 1 to 5 ng mL-1 with a low limit of detection of 0.8 pg mL-1.					
36144631	3	75	dep	oxide	671:675	arg1	GO					678:679	GO	678:679	GO	678:679	Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.					
36144631	2	76	theme	amperometric	500:511	arg1	determination					513:525	sensitive amperometric determination	490:525	sensitive amperometric determination of PSA	490:532	In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA.					
36144631	0	77	from	Immunosensor	35:46	arg1	Electrodes					92:101	Chitosan-Graphene-Modified Patterned ITO Electrodes	51:101	Chitosan-Graphene-Modified Patterned ITO Electrodes	51:101	Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.					
36144631	4	78	theme	high	795:798	arg1	biocompatibility					800:815	high biocompatibility	795:815	high biocompatibility	795:815	When CS-rGO was modified on the patterned ITO, CS offered high biocompatibility and reactive groups for the immobilization of recognition antibodies and rGO acted as a transduction element and enhancer to improve the electronic conductivity and stability of the CS-rGO composite film.					
36144631	7	79	theme	high	1490:1493	arg1	selectivity					1495:1505	high selectivity	1490:1505	high selectivity	1490:1505	This sensor also exhibited high selectivity, reproducibility, and good storage stability.					
36144631	1	80	theme	prostate-specific	181:197	arg1	PSA					208:210	PSA	208:210	PSA	208:210	A facile and highly sensitive determination of prostate-specific antigen (PSA) is of great significance for the early diagnosis, monitoring and prognosis of prostate cancer.					
36144631	1	80	theme	prostate-specific	181:197	arg1	antigen					199:205	prostate-specific antigen	181:205	prostate-specific antigen (PSA)	181:211	A facile and highly sensitive determination of prostate-specific antigen (PSA) is of great significance for the early diagnosis, monitoring and prognosis of prostate cancer.					
36144631	8	81	theme	serum	1658:1662	arg1	samples					1664:1670	spiked human serum samples	1645:1670	spiked human serum samples	1645:1670	The application of the prepared immunosensor was successfully validated by measuring PSA in spiked human serum samples.					
36144631	8	82	theme	prepared	1576:1583	arg1	immunosensor					1585:1596	the prepared immunosensor	1572:1596	the prepared immunosensor	1572:1596	The application of the prepared immunosensor was successfully validated by measuring PSA in spiked human serum samples.					
35354766	0	0	theme	cibaria	84:90	arg1	Biomass					97:103	Probiotic Weissella cibaria JW15 Biomass	64:103	Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology	64:169	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.					
35354766	4	1	theme	medium	794:799	arg1	22.35					810:814	22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80	810:974	22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80	810:974	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	1	theme	medium	794:799	arg1	composition					767:777	The composition	763:777	The composition of the optimal medium (OM)	763:804	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	9	2	theme	pH	1584:1585	arg1	control					1587:1593	the pH control	1580:1593	the pH control	1580:1593	The biomass production of JW15 significantly improved (1.98 times higher) under the pH control, and the cost of OM was reduced by two-thirds compared to that in the MRS medium.					
35354766	1	3	theme	biomass	257:263	arg1	production					265:274	the biomass production	253:274	the biomass production of Weissella cibaria JW15 (JW15)	253:307	The objective of this study was to optimize industrial-grade media for improving the biomass production of Weissella cibaria JW15 (JW15) using a statistical approach.					
35354766	0	4	theme	Weissella	74:82	arg1	Biomass					97:103	Probiotic Weissella cibaria JW15 Biomass	64:103	Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology	64:169	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.					
35354766	6	5	theme	JW15	1127:1130	arg1	g/l					1141:1143	4.89 g/l	1136:1143	4.89 g/l	1136:1143	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	6	5	theme	JW15	1127:1130	arg1	weight					1117:1122	the dry cell weight	1104:1122	the dry cell weight of JW15	1104:1130	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	2	6	theme	L-cysteine	550:559	arg1	phosphate					561:569	L-cysteine phosphate	550:569	L-cysteine phosphate	550:569	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	6	7	dep	g/l	1141:1143	arg1	higher					1217:1222	higher	1217:1222	higher	1217:1222	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	6	7	dep	g/l	1141:1143	arg1	comparable					1156:1165	comparable	1156:1165	comparable	1156:1165	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	6	8	theme	cell	1112:1115	arg1	g/l					1141:1143	4.89 g/l	1136:1143	4.89 g/l	1136:1143	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	6	8	theme	cell	1112:1115	arg1	weight					1117:1122	the dry cell weight	1104:1122	the dry cell weight of JW15	1104:1130	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	7	9	from	production	1315:1324	arg1	OM					1359:1360	the OM	1355:1360	the OM	1355:1360	Correspondingly, JW15 showed a rapid and increased production of lactic and acetic acid in the OM.					
35354766	4	10	theme	g/l	919:921	arg1	MgSO4·7H2O					923:932	0.1 g/l MgSO4·7H2O	915:932	0.1 g/l MgSO4·7H2O	915:932	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	5	11	theme	established	1046:1056	arg1	MRS					1077:1079	MRS	1077:1079	MRS	1077:1079	The OM significantly improved the biomass production of JW15 over an established commercial medium (MRS).					
35354766	5	11	theme	established	1046:1056	arg1	medium					1069:1074	an established commercial medium	1043:1074	an established commercial medium (MRS)	1043:1080	The OM significantly improved the biomass production of JW15 over an established commercial medium (MRS).					
35354766	0	12	theme	JW15	92:95	arg1	Biomass					97:103	Probiotic Weissella cibaria JW15 Biomass	64:103	Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology	64:169	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.					
35354766	3	13	from	variables	714:722	arg1	RSM					757:759	RSM	757:759	RSM	757:759	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	3	13	from	variables	714:722	arg1	methodology					744:754	response surface methodology	727:754	response surface methodology (RSM)	727:760	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	5	14	theme	commercial	1058:1067	arg1	MRS					1077:1079	MRS	1077:1079	MRS	1077:1079	The OM significantly improved the biomass production of JW15 over an established commercial medium (MRS).					
35354766	5	14	theme	commercial	1058:1067	arg1	medium					1069:1074	an established commercial medium	1043:1074	an established commercial medium (MRS)	1043:1080	The OM significantly improved the biomass production of JW15 over an established commercial medium (MRS).					
35354766	2	15	dep	sources	514:520	arg1	K2HPO4					523:528	K2HPO4	523:528	K2HPO4	523:528	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	16	theme	mineral	506:512	arg1	sources					514:520	five mineral sources	501:520	five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4)	501:588	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	17	dep	sources	380:386	arg1	fructose					398:405	fructose	398:405	fructose	398:405	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	17	dep	sources	380:386	arg1	sucrose					412:418	sucrose	412:418	sucrose	412:418	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	17	dep	sources	380:386	arg1	glucose					389:395	glucose	389:395	glucose	389:395	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	17	dep	sources	380:386	arg1	sources					380:386	three carbon sources	367:386	three carbon sources (glucose, fructose, and sucrose)	367:419	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	8	18	theme	5-l	1434:1436	arg1	bioreactor					1438:1447	a 5-l bioreactor	1432:1447	a 5-l bioreactor	1432:1447	To perform a scale-up validation, batch fermentation was executed in a 5-l bioreactor at 37°C with or without a pH control at 6.0 ± 0.1.					
35354766	10	19	theme	mass	1715:1718	arg1	JW15					1730:1733	mass producing JW15	1715:1733	mass producing JW15 for industrial use	1715:1752	In conclusion, OM may be utilized for mass producing JW15 for industrial use.					
35354766	2	20	theme	soy	483:485	arg1	peptone					487:493	soy peptone	483:493	soy peptone	483:493	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	20	theme	soy	483:485	arg1	sources					437:443	three nitrogen sources	422:443	three nitrogen sources (protease peptone, yeast extract, and soy peptone)	422:494	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	6	21	theme	fermenting	1089:1098	arg1	OM					1100:1101	fermenting OM	1089:1101	fermenting OM	1089:1101	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	2	22	theme	Plackett-Burman	617:631	arg1	design					633:638	the Plackett-Burman design	613:638	the Plackett-Burman design	613:638	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	4	23	theme	5.0	891:893	arg1	g/l					895:897	g/l	895:897	g/l	895:897	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	24	theme	g/l	835:837	arg1	sucrose					839:845	15.57 g/l sucrose	829:845	15.57 g/l sucrose	829:845	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	25	theme	10.05	852:856	arg1	peptone					866:872	10.05 g/l soy peptone	852:872	10.05 g/l soy peptone	852:872	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	26	theme	g/l	895:897	arg1	acetate					906:912	5.0 g/l sodium acetate	891:912	5.0 g/l sodium acetate	891:912	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	2	27	theme	yeast	464:468	arg1	sources					437:443	three nitrogen sources	422:443	three nitrogen sources (protease peptone, yeast extract, and soy peptone)	422:494	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	27	theme	yeast	464:468	arg1	extract					470:476	yeast extract	464:476	yeast extract	464:476	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	28	dep	sources	437:443	arg1	peptone					487:493	soy peptone	483:493	soy peptone	483:493	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	28	dep	sources	437:443	arg1	sources					437:443	three nitrogen sources	422:443	three nitrogen sources (protease peptone, yeast extract, and soy peptone)	422:494	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	28	dep	sources	437:443	arg1	peptone					455:461	protease peptone	446:461	protease peptone	446:461	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	28	dep	sources	437:443	arg1	extract					470:476	yeast extract	464:476	yeast extract	464:476	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	29	dep	K2HPO4	523:528	arg1	MgSO4					572:576	MgSO4	572:576	MgSO4	572:576	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	29	dep	K2HPO4	523:528	arg1	citrate					541:547	potassium citrate	531:547	potassium citrate	531:547	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	29	dep	K2HPO4	523:528	arg1	phosphate					561:569	L-cysteine phosphate	550:569	L-cysteine phosphate	550:569	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	29	dep	K2HPO4	523:528	arg1	MnSO4					583:587	MnSO4	583:587	MnSO4	583:587	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	5	30	theme	biomass	1011:1017	arg1	production					1019:1028	the biomass production	1007:1028	the biomass production of JW15	1007:1036	The OM significantly improved the biomass production of JW15 over an established commercial medium (MRS).					
35354766	2	31	theme	nitrogen	428:435	arg1	peptone					487:493	soy peptone	483:493	soy peptone	483:493	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	31	theme	nitrogen	428:435	arg1	sources					437:443	three nitrogen sources	422:443	three nitrogen sources (protease peptone, yeast extract, and soy peptone)	422:494	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	31	theme	nitrogen	428:435	arg1	peptone					455:461	protease peptone	446:461	protease peptone	446:461	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	31	theme	nitrogen	428:435	arg1	extract					470:476	yeast extract	464:476	yeast extract	464:476	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	1	32	theme	cibaria	289:295	arg1	production					265:274	the biomass production	253:274	the biomass production of Weissella cibaria JW15 (JW15)	253:307	The objective of this study was to optimize industrial-grade media for improving the biomass production of Weissella cibaria JW15 (JW15) using a statistical approach.					
35354766	9	33	dep	improved	1545:1552	arg1	higher					1566:1571	higher	1566:1571	higher	1566:1571	The biomass production of JW15 significantly improved (1.98 times higher) under the pH control, and the cost of OM was reduced by two-thirds compared to that in the MRS medium.					
35354766	9	34	theme	OM	1612:1613	arg1	cost					1604:1607	the cost	1600:1607	the cost of OM	1600:1613	The biomass production of JW15 significantly improved (1.98 times higher) under the pH control, and the cost of OM was reduced by two-thirds compared to that in the MRS medium.					
35354766	1	35	theme	study	194:198	arg1	objective					176:184	The objective	172:184	The objective of this study	172:198	The objective of this study was to optimize industrial-grade media for improving the biomass production of Weissella cibaria JW15 (JW15) using a statistical approach.					
35354766	0	36	theme	Industrial	19:28	arg1	Medium					30:35	an Industrial Medium	16:35	an Industrial Medium	16:35	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.					
35354766	6	37	theme	dry	1108:1110	arg1	g/l					1141:1143	4.89 g/l	1136:1143	4.89 g/l	1136:1143	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	6	37	theme	dry	1108:1110	arg1	weight					1117:1122	the dry cell weight	1104:1122	the dry cell weight of JW15	1104:1130	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	4	38	theme	optimal	786:792	arg1	medium					794:799	the optimal medium	782:799	the optimal medium (OM)	782:804	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	38	theme	optimal	786:792	arg1	OM					802:803	OM	802:803	OM	802:803	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	7	39	theme	acetic	1340:1345	arg1	acid					1347:1350	lactic and acetic acid	1329:1350	lactic and acetic acid	1329:1350	Correspondingly, JW15 showed a rapid and increased production of lactic and acetic acid in the OM.					
35354766	4	40	theme	sodium	899:904	arg1	acetate					906:912	5.0 g/l sodium acetate	891:912	5.0 g/l sodium acetate	891:912	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	0	41	theme	Plackett-Burman	115:129	arg1	Design					131:136	the Plackett-Burman Design	111:136	the Plackett-Burman Design	111:136	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.					
35354766	4	42	theme	15.57	829:833	arg1	g/l					835:837	g/l	835:837	g/l	835:837	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	10	43	used	utilized	1702:1709	arg2	OM					1692:1693	OM	1692:1693	OM	1692:1693	In conclusion, OM may be utilized for mass producing JW15 for industrial use.					
35354766	4	44	theme	soy	862:864	arg1	peptone					866:872	10.05 g/l soy peptone	852:872	10.05 g/l soy peptone	852:872	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	45	theme	g/l	879:881	arg1	K2HPO4					883:888	2.0 g/l K2HPO4	875:888	2.0 g/l K2HPO4	875:888	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	3	46	theme	soy	677:679	arg1	peptone					681:687	soy peptone	677:687	soy peptone	677:687	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	3	46	theme	soy	677:679	arg1	sucrose					664:670	sucrose	664:670	sucrose	664:670	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	3	46	theme	soy	677:679	arg1	glucose					655:661	glucose	655:661	glucose	655:661	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	3	46	theme	soy	677:679	arg1	variables					714:722	significant variables	702:722	significant variables in response surface methodology (RSM)	702:760	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	0	47	theme	Response	142:149	arg1	Methodology					159:169	Response Surface Methodology	142:169	Response Surface Methodology	142:169	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.					
35354766	10	48	theme	industrial	1739:1748	arg1	use					1750:1752	industrial use	1739:1752	industrial use	1739:1752	In conclusion, OM may be utilized for mass producing JW15 for industrial use.					
35354766	8	49	theme	batch	1397:1401	arg1	fermentation					1403:1414	batch fermentation	1397:1414	batch fermentation	1397:1414	To perform a scale-up validation, batch fermentation was executed in a 5-l bioreactor at 37°C with or without a pH control at 6.0 ± 0.1.					
35354766	0	50	theme	Medium	30:35	arg1	Optimization					0:11	Optimization	0:11	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.	0:170	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.					
35354766	3	51	theme	significant	702:712	arg1	peptone					681:687	soy peptone	677:687	soy peptone	677:687	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	3	51	theme	significant	702:712	arg1	sucrose					664:670	sucrose	664:670	sucrose	664:670	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	3	51	theme	significant	702:712	arg1	glucose					655:661	glucose	655:661	glucose	655:661	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	3	51	theme	significant	702:712	arg1	variables					714:722	significant variables	702:722	significant variables in response surface methodology (RSM)	702:760	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	4	52	theme	2.0	875:877	arg1	g/l					879:881	g/l	879:881	g/l	879:881	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	53	dep	g/l	963:965	arg1	Tween					967:971	Tween	967:971	Tween	967:971	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	1	54	theme	industrial-grade	216:231	arg1	media					233:237	industrial-grade media	216:237	industrial-grade media for improving the biomass production of Weissella cibaria JW15 (JW15) using a statistical approach	216:336	The objective of this study was to optimize industrial-grade media for improving the biomass production of Weissella cibaria JW15 (JW15) using a statistical approach.					
35354766	0	55	theme	Conditions	49:58	arg1	Optimization					0:11	Optimization	0:11	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.	0:170	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.					
35354766	9	56	theme	JW15	1526:1529	arg1	production					1512:1521	The biomass production	1500:1521	The biomass production of JW15	1500:1529	The biomass production of JW15 significantly improved (1.98 times higher) under the pH control, and the cost of OM was reduced by two-thirds compared to that in the MRS medium.					
35354766	2	57	theme	potassium	531:539	arg1	citrate					541:547	potassium citrate	531:547	potassium citrate	531:547	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	4	58	theme	g/l	858:860	arg1	peptone					866:872	10.05 g/l soy peptone	852:872	10.05 g/l soy peptone	852:872	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	8	59	theme	scale-up	1376:1383	arg1	validation					1385:1394	a scale-up validation	1374:1394	a scale-up validation	1374:1394	To perform a scale-up validation, batch fermentation was executed in a 5-l bioreactor at 37°C with or without a pH control at 6.0 ± 0.1.					
35354766	8	60	theme	pH	1475:1476	arg1	control					1478:1484	a pH control	1473:1484	a pH control at 6.0 ± 0.1	1473:1497	To perform a scale-up validation, batch fermentation was executed in a 5-l bioreactor at 37°C with or without a pH control at 6.0 ± 0.1.					
35354766	0	61	theme	Culture	41:47	arg1	Conditions					49:58	Culture Conditions	41:58	Culture Conditions	41:58	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.					
35354766	5	62	theme	JW15	1033:1036	arg1	production					1019:1028	the biomass production	1007:1028	the biomass production of JW15	1007:1036	The OM significantly improved the biomass production of JW15 over an established commercial medium (MRS).					
35354766	0	63	theme	Surface	151:157	arg1	Methodology					159:169	Response Surface Methodology	142:169	Response Surface Methodology	142:169	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.					
35354766	4	64	theme	0.05	935:938	arg1	g/l					940:942	g/l	940:942	g/l	940:942	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	1	65	theme	statistical	317:327	arg1	approach					329:336	a statistical approach	315:336	a statistical approach	315:336	The objective of this study was to optimize industrial-grade media for improving the biomass production of Weissella cibaria JW15 (JW15) using a statistical approach.					
35354766	4	66	theme	g/l	816:818	arg1	glucose					820:826	g/l glucose	816:826	g/l glucose	816:826	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	3	67	used	used	694:697	arg2	variables					714:722	significant variables	702:722	significant variables in response surface methodology (RSM)	702:760	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	3	67	used	used	694:697	arg2	sucrose					664:670	sucrose	664:670	sucrose	664:670	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	3	67	used	used	694:697	arg2	glucose					655:661	glucose	655:661	glucose	655:661	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	3	67	used	used	694:697	arg2	peptone					681:687	soy peptone	677:687	soy peptone	677:687	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	10	68	theme	producing	1720:1728	arg1	JW15					1730:1733	mass producing JW15	1715:1733	mass producing JW15 for industrial use	1715:1752	In conclusion, OM may be utilized for mass producing JW15 for industrial use.					
35354766	3	69	theme	surface	736:742	arg1	RSM					757:759	RSM	757:759	RSM	757:759	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	3	69	theme	surface	736:742	arg1	methodology					744:754	response surface methodology	727:754	response surface methodology (RSM)	727:760	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	9	70	theme	biomass	1504:1510	arg1	production					1512:1521	The biomass production	1500:1521	The biomass production of JW15	1500:1529	The biomass production of JW15 significantly improved (1.98 times higher) under the pH control, and the cost of OM was reduced by two-thirds compared to that in the MRS medium.					
35354766	7	71	theme	acid	1347:1350	arg1	production					1315:1324	production	1315:1324	production of lactic and acetic acid in the OM	1315:1360	Correspondingly, JW15 showed a rapid and increased production of lactic and acetic acid in the OM.					
35354766	0	72	theme	Probiotic	64:72	arg1	Biomass					97:103	Probiotic Weissella cibaria JW15 Biomass	64:103	Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology	64:169	Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology.					
35354766	8	73	from	±	1493:1493	arg1	control					1478:1484	a pH control	1473:1484	a pH control at 6.0 ± 0.1	1473:1497	To perform a scale-up validation, batch fermentation was executed in a 5-l bioreactor at 37°C with or without a pH control at 6.0 ± 0.1.					
35354766	6	74	theme	predicted	1174:1182	arg1	value					1184:1188	the predicted value	1170:1188	the predicted value (4.77 g/l)	1170:1199	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	6	74	theme	predicted	1174:1182	arg1	g/l					1196:1198	4.77 g/l	1191:1198	4.77 g/l	1191:1198	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	6	75	theme	MRS	1241:1243	arg1	medium					1245:1250	the MRS medium	1237:1250	the MRS medium (3.02 g/l)	1237:1261	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	6	75	theme	MRS	1241:1243	arg1	g/l					1258:1260	3.02 g/l	1253:1260	3.02 g/l	1253:1260	After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l).					
35354766	4	76	theme	g/l	940:942	arg1	MnSO4·H2O					944:952	0.05 g/l MnSO4·H2O	935:952	0.05 g/l MnSO4·H2O	935:952	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	7	77	theme	lactic	1329:1334	arg1	acid					1347:1350	lactic and acetic acid	1329:1350	lactic and acetic acid	1329:1350	Correspondingly, JW15 showed a rapid and increased production of lactic and acetic acid in the OM.					
35354766	2	78	theme	protease	446:453	arg1	sources					437:443	three nitrogen sources	422:443	three nitrogen sources (protease peptone, yeast extract, and soy peptone)	422:494	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	78	theme	protease	446:453	arg1	peptone					455:461	protease peptone	446:461	protease peptone	446:461	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	4	79	dep	22.35	810:814	arg1	K2HPO4					883:888	2.0 g/l K2HPO4	875:888	2.0 g/l K2HPO4	875:888	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	79	dep	22.35	810:814	arg1	MgSO4·7H2O					923:932	0.1 g/l MgSO4·7H2O	915:932	0.1 g/l MgSO4·7H2O	915:932	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	79	dep	22.35	810:814	arg1	peptone					866:872	10.05 g/l soy peptone	852:872	10.05 g/l soy peptone	852:872	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	79	dep	22.35	810:814	arg1	sucrose					839:845	15.57 g/l sucrose	829:845	15.57 g/l sucrose	829:845	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	79	dep	22.35	810:814	arg1	g/l					963:965	1.0 g/l	959:965	1.0 g/l Tween 80	959:974	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	79	dep	22.35	810:814	arg1	acetate					906:912	5.0 g/l sodium acetate	891:912	5.0 g/l sodium acetate	891:912	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	79	dep	22.35	810:814	arg1	glucose					820:826	g/l glucose	816:826	g/l glucose	816:826	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	4	79	dep	22.35	810:814	arg1	MnSO4·H2O					944:952	0.05 g/l MnSO4·H2O	935:952	0.05 g/l MnSO4·H2O	935:952	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	9	80	theme	MRS	1665:1667	arg1	medium					1669:1674	the MRS medium	1661:1674	the MRS medium	1661:1674	The biomass production of JW15 significantly improved (1.98 times higher) under the pH control, and the cost of OM was reduced by two-thirds compared to that in the MRS medium.					
35354766	2	81	theme	carbon	373:378	arg1	fructose					398:405	fructose	398:405	fructose	398:405	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	81	theme	carbon	373:378	arg1	sucrose					412:418	sucrose	412:418	sucrose	412:418	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	81	theme	carbon	373:378	arg1	glucose					389:395	glucose	389:395	glucose	389:395	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	2	81	theme	carbon	373:378	arg1	sources					380:386	three carbon sources	367:386	three carbon sources (glucose, fructose, and sucrose)	367:419	Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, L-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design.					
35354766	4	82	theme	0.1	915:917	arg1	g/l					919:921	g/l	919:921	g/l	919:921	The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80.					
35354766	3	83	theme	response	727:734	arg1	RSM					757:759	RSM	757:759	RSM	757:759	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
35354766	3	83	theme	response	727:734	arg1	methodology					744:754	response surface methodology	727:754	response surface methodology (RSM)	727:760	Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM).					
36273496	0	0	theme	leptin	56:61	arg1	resistance					63:72	leptin resistance	56:72	leptin resistance	56:72	Sucrose solution, but not liquid sucrose diet, leads to leptin resistance irrespective of the time of day that sucrose is available.					
36273496	6	1	theme	resistance	1169:1178	arg1	development					1147:1157	development	1147:1157	development of leptin resistance	1147:1178	Because LiqS rats consume sucrose throughout the day and night we tested whether limiting sucrose solution access to either the light or dark period prevented development of leptin resistance.					
36273496	6	2	theme	sucrose	1078:1084	arg1	access					1095:1100	sucrose solution access	1078:1100	sucrose solution access to either the light or dark period	1078:1135	Because LiqS rats consume sucrose throughout the day and night we tested whether limiting sucrose solution access to either the light or dark period prevented development of leptin resistance.					
36273496	3	3	theme	Female	455:460	arg1	rats					477:480	Female Sprague Dawley rats	455:480	Female Sprague Dawley rats	455:480	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	3	4	theme	NS	593:594	arg1	NSLiq					617:621	NSLiq	617:621	NSLiq	617:621	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	3	4	theme	NS	593:594	arg1	diet					596:599	NS diet	593:599	NS diet in liquid form (NSLiq)	593:622	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	1	5	theme	composite	208:216	arg1	diet					218:221	a sucrose-free composite diet	193:221	a sucrose-free composite diet	193:221	Rats offered free access to sucrose solution in addition to a sucrose-free composite diet develop leptin resistance whereas those consuming a similar amount of sucrose from a dry diet remain leptin responsive.					
36273496	4	6	theme	much	785:788	arg1	sucrose					790:796	much sucrose	785:796	much sucrose	785:796	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	2	7	from	diet	405:408	arg1	form					420:423	liquid form	413:423	liquid form	413:423	Here we tested whether rats consuming a complete high sucrose diet in liquid form also became leptin resistant.					
36273496	6	8	dep	period	1130:1135	arg1	either					1105:1110	either	1105:1110	either	1105:1110	Because LiqS rats consume sucrose throughout the day and night we tested whether limiting sucrose solution access to either the light or dark period prevented development of leptin resistance.					
36273496	4	9	theme	body	853:856	arg1	fat					858:860	body fat	853:860	body fat	853:860	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	1	10	theme	dry	308:310	arg1	diet					312:315	a dry diet	306:315	a dry diet	306:315	Rats offered free access to sucrose solution in addition to a sucrose-free composite diet develop leptin resistance whereas those consuming a similar amount of sucrose from a dry diet remain leptin responsive.					
36273496	9	11	theme	serum	1605:1609	arg1	concentration					1618:1630	serum leptin concentration	1605:1630	serum leptin concentration	1605:1630	The results of this study show that leptin resistance of LiqS rats is independent of when the sucrose is consumed and is unrelated to total energy intake, body fat mass or serum leptin concentration.					
36273496	7	12	theme	free	1226:1229	arg1	access					1231:1236	free access	1226:1236	free access to sucrose	1226:1247	Leptin resistant LiqS rats were either given free access to sucrose, had access to sucrose only at night or had access only during the day.					
36273496	9	13	theme	leptin	1611:1616	arg1	concentration					1618:1630	serum leptin concentration	1605:1630	serum leptin concentration	1605:1630	The results of this study show that leptin resistance of LiqS rats is independent of when the sucrose is consumed and is unrelated to total energy intake, body fat mass or serum leptin concentration.					
36273496	8	14	theme	those	1393:1397	arg1	level					1384:1388	the level	1380:1388	the level of those with free access	1380:1414	The intake of rats with limited access was supplemented to the level of those with free access by tube-feeding.					
36273496	3	15	theme	sucrose	568:574	arg1	solution					576:583	30% sucrose solution	564:583	30% sucrose solution	564:583	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	9	16	theme	study	1453:1457	arg1	results					1437:1443	The results	1433:1443	The results of this study	1433:1457	The results of this study show that leptin resistance of LiqS rats is independent of when the sucrose is consumed and is unrelated to total energy intake, body fat mass or serum leptin concentration.					
36273496	3	17	theme	sucrose	497:503	arg1	diet					510:513	a sucrose free diet	495:513	a sucrose free diet (NS)	495:518	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	3	17	theme	sucrose	497:503	arg1	NS					516:517	NS	516:517	NS	516:517	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	1	18	theme	leptin	231:236	arg1	resistance					238:247	leptin resistance	231:247	leptin resistance	231:247	Rats offered free access to sucrose solution in addition to a sucrose-free composite diet develop leptin resistance whereas those consuming a similar amount of sucrose from a dry diet remain leptin responsive.					
36273496	4	19	theme	greatest	834:841	arg1	amount					843:848	the greatest amount	830:848	the greatest amount of body fat	830:860	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	4	19	theme	greatest	834:841	arg1	fat					858:860	body fat	853:860	body fat	853:860	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	7	20	contain	had	1289:1291	arg1	rats					1203:1206	Leptin resistant LiqS rats	1181:1206	Leptin resistant LiqS rats	1181:1206	Leptin resistant LiqS rats were either given free access to sucrose, had access to sucrose only at night or had access only during the day.					
36273496	7	20	contain	had	1289:1291	arg2	access					1293:1298	access	1293:1298	access	1293:1298	Leptin resistant LiqS rats were either given free access to sucrose, had access to sucrose only at night or had access only during the day.					
36273496	3	21	from	diet	596:599	arg1	form					611:614	liquid form	604:614	liquid form	604:614	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	5	22	theme	leptin	889:894	arg1	resistance					896:905	leptin resistance	889:905	leptin resistance	889:905	Therefore, development of leptin resistance is dependent upon the consumption of sucrose independent of any other nutrients.					
36273496	4	23	theme	LiqS	801:804	arg1	rats					806:809	LiqS rats	801:809	LiqS rats	801:809	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	4	24	contain	had	826:828	arg1	rats					768:771	HSLiq rats	762:771	HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats	762:824	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	4	24	contain	had	826:828	arg2	fat					858:860	body fat	853:860	body fat	853:860	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	4	24	contain	had	826:828	arg2	amount					843:848	the greatest amount	830:848	the greatest amount of body fat	830:860	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	0	25	theme	Sucrose	0:6	arg1	solution					8:15	Sucrose solution	0:15	Sucrose solution	0:15	Sucrose solution, but not liquid sucrose diet, leads to leptin resistance irrespective of the time of day that sucrose is available.					
36273496	3	26	from	diet	540:543	arg1	form					611:614	liquid form	604:614	liquid form	604:614	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	8	27	theme	limited	1345:1351	arg1	access					1353:1358	limited access	1345:1358	limited access	1345:1358	The intake of rats with limited access was supplemented to the level of those with free access by tube-feeding.					
36273496	5	28	theme	resistance	896:905	arg1	development					874:884	development	874:884	development of leptin resistance	874:905	Therefore, development of leptin resistance is dependent upon the consumption of sucrose independent of any other nutrients.					
36273496	4	29	theme	LiqS	673:676	arg1	rats					678:681	LiqS rats	673:681	LiqS rats	673:681	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	9	30	theme	LiqS	1490:1493	arg1	rats					1495:1498	LiqS rats	1490:1498	LiqS rats	1490:1498	The results of this study show that leptin resistance of LiqS rats is independent of when the sucrose is consumed and is unrelated to total energy intake, body fat mass or serum leptin concentration.					
36273496	5	31	theme	independent	952:962	arg1	consumption					929:939	the consumption	925:939	the consumption of sucrose independent of any other nutrients	925:985	Therefore, development of leptin resistance is dependent upon the consumption of sucrose independent of any other nutrients.					
36273496	1	32	theme	free	146:149	arg1	access					151:156	free access	146:156	free access to sucrose solution	146:176	Rats offered free access to sucrose solution in addition to a sucrose-free composite diet develop leptin resistance whereas those consuming a similar amount of sucrose from a dry diet remain leptin responsive.					
36273496	2	33	theme	sucrose	397:403	arg1	diet					405:408	a complete high sucrose diet	381:408	a complete high sucrose diet in liquid form	381:423	Here we tested whether rats consuming a complete high sucrose diet in liquid form also became leptin resistant.					
36273496	4	34	theme	NSLiq	815:819	arg1	rats					821:824	NSLiq rats	815:824	NSLiq rats	815:824	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	2	35	theme	high	392:395	arg1	diet					405:408	a complete high sucrose diet	381:408	a complete high sucrose diet in liquid form	381:423	Here we tested whether rats consuming a complete high sucrose diet in liquid form also became leptin resistant.					
36273496	8	36	with	rats	1335:1338	arg1	access					1353:1358	limited access	1345:1358	limited access	1345:1358	The intake of rats with limited access was supplemented to the level of those with free access by tube-feeding.					
36273496	6	37	theme	dark	1125:1128	arg1	period					1130:1135	either the light or dark period	1105:1135	period	1130:1135	Because LiqS rats consume sucrose throughout the day and night we tested whether limiting sucrose solution access to either the light or dark period prevented development of leptin resistance.					
36273496	3	38	theme	free	505:508	arg1	diet					510:513	a sucrose free diet	495:513	a sucrose free diet (NS)	495:518	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	3	38	theme	free	505:508	arg1	NS					516:517	NS	516:517	NS	516:517	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	9	39	theme	energy	1573:1578	arg1	intake					1580:1585	total energy intake	1567:1585	total energy intake	1567:1585	The results of this study show that leptin resistance of LiqS rats is independent of when the sucrose is consumed and is unrelated to total energy intake, body fat mass or serum leptin concentration.					
36273496	2	40	theme	complete	383:390	arg1	diet					405:408	a complete high sucrose diet	381:408	a complete high sucrose diet in liquid form	381:423	Here we tested whether rats consuming a complete high sucrose diet in liquid form also became leptin resistant.					
36273496	5	41	theme	other	971:975	arg1	nutrients					977:985	any other nutrients	967:985	any other nutrients	967:985	Therefore, development of leptin resistance is dependent upon the consumption of sucrose independent of any other nutrients.					
36273496	7	42	theme	LiqS	1198:1201	arg1	rats					1203:1206	Leptin resistant LiqS rats	1181:1206	Leptin resistant LiqS rats	1181:1206	Leptin resistant LiqS rats were either given free access to sucrose, had access to sucrose only at night or had access only during the day.					
36273496	1	43	theme	sucrose	161:167	arg1	solution					169:176	sucrose solution	161:176	sucrose solution	161:176	Rats offered free access to sucrose solution in addition to a sucrose-free composite diet develop leptin resistance whereas those consuming a similar amount of sucrose from a dry diet remain leptin responsive.					
36273496	0	44	theme	liquid	26:31	arg1	diet					41:44	liquid sucrose diet	26:44	liquid sucrose diet	26:44	Sucrose solution, but not liquid sucrose diet, leads to leptin resistance irrespective of the time of day that sucrose is available.					
36273496	3	45	theme	liquid	604:609	arg1	form					611:614	liquid form	604:614	liquid form	604:614	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	6	46	theme	light	1116:1120	arg1	period					1130:1135	either the light or dark period	1105:1135	period	1130:1135	Because LiqS rats consume sucrose throughout the day and night we tested whether limiting sucrose solution access to either the light or dark period prevented development of leptin resistance.					
36273496	3	47	theme	NS	551:552	arg1	LiqS					586:589	LiqS	586:589	LiqS	586:589	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	3	47	theme	NS	551:552	arg1	diet					554:557	NS diet	551:557	NS diet	551:557	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	7	48	theme	resistant	1188:1196	arg1	rats					1203:1206	Leptin resistant LiqS rats	1181:1206	Leptin resistant LiqS rats	1181:1206	Leptin resistant LiqS rats were either given free access to sucrose, had access to sucrose only at night or had access only during the day.					
36273496	9	49	theme	fat	1593:1595	arg1	mass					1597:1600	body fat mass	1588:1600	body fat mass	1588:1600	The results of this study show that leptin resistance of LiqS rats is independent of when the sucrose is consumed and is unrelated to total energy intake, body fat mass or serum leptin concentration.					
36273496	3	50	from	solution	576:583	arg1	form					611:614	liquid form	604:614	liquid form	604:614	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	8	51	theme	rats	1335:1338	arg1	intake					1325:1330	The intake	1321:1330	The intake of rats with limited access	1321:1358	The intake of rats with limited access was supplemented to the level of those with free access by tube-feeding.					
36273496	0	52	theme	day	102:104	arg1	time					94:97	the time	90:97	the time of day	90:104	Sucrose solution, but not liquid sucrose diet, leads to leptin resistance irrespective of the time of day that sucrose is available.					
36273496	3	53	theme	dry	523:525	arg1	HS					546:547	HS	546:547	HS	546:547	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	3	53	theme	dry	523:525	arg1	diet					540:543	a dry high sucrose diet	521:543	a dry high sucrose diet (HS)	521:548	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	5	54	theme	nutrients	977:985	arg1	independent					952:962	independent	952:962	independent	952:962	Therefore, development of leptin resistance is dependent upon the consumption of sucrose independent of any other nutrients.					
36273496	4	55	theme	HSLiq	762:766	arg1	rats					768:771	HSLiq rats	762:771	HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats	762:824	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	2	56	theme	liquid	413:418	arg1	form					420:423	liquid form	413:423	liquid form	413:423	Here we tested whether rats consuming a complete high sucrose diet in liquid form also became leptin resistant.					
36273496	1	57	theme	similar	275:281	arg1	sucrose					293:299	sucrose	293:299	sucrose	293:299	Rats offered free access to sucrose solution in addition to a sucrose-free composite diet develop leptin resistance whereas those consuming a similar amount of sucrose from a dry diet remain leptin responsive.					
36273496	1	57	theme	similar	275:281	arg1	amount					283:288	a similar amount	273:288	a similar amount of sucrose	273:299	Rats offered free access to sucrose solution in addition to a sucrose-free composite diet develop leptin resistance whereas those consuming a similar amount of sucrose from a dry diet remain leptin responsive.					
36273496	3	58	from	diet	630:633	arg1	form					611:614	liquid form	604:614	liquid form	604:614	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	9	59	theme	total	1567:1571	arg1	intake					1580:1585	total energy intake	1567:1585	total energy intake	1567:1585	The results of this study show that leptin resistance of LiqS rats is independent of when the sucrose is consumed and is unrelated to total energy intake, body fat mass or serum leptin concentration.					
36273496	0	60	theme	sucrose	33:39	arg1	diet					41:44	liquid sucrose diet	26:44	liquid sucrose diet	26:44	Sucrose solution, but not liquid sucrose diet, leads to leptin resistance irrespective of the time of day that sucrose is available.					
36273496	5	61	theme	sucrose	944:950	arg1	consumption					929:939	the consumption	925:939	the consumption of sucrose independent of any other nutrients	925:985	Therefore, development of leptin resistance is dependent upon the consumption of sucrose independent of any other nutrients.					
36273496	9	62	theme	leptin	1469:1474	arg1	resistance					1476:1485	leptin resistance	1469:1485	leptin resistance of LiqS rats	1469:1498	The results of this study show that leptin resistance of LiqS rats is independent of when the sucrose is consumed and is unrelated to total energy intake, body fat mass or serum leptin concentration.					
36273496	3	63	theme	30	564:565	arg1	%					566:566	%	566:566	%	566:566	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	8	64	theme	free	1404:1407	arg1	access					1409:1414	free access	1404:1414	free access	1404:1414	The intake of rats with limited access was supplemented to the level of those with free access by tube-feeding.					
36273496	3	65	theme	sucrose	532:538	arg1	HS					546:547	HS	546:547	HS	546:547	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	3	65	theme	sucrose	532:538	arg1	diet					540:543	a dry high sucrose diet	521:543	a dry high sucrose diet (HS)	521:548	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	4	66	theme	other	714:718	arg1	groups					720:725	all other groups	710:725	all other groups	710:725	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	3	67	theme	%	566:566	arg1	solution					576:583	30% sucrose solution	564:583	30% sucrose solution	564:583	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	7	68	theme	Leptin	1181:1186	arg1	rats					1203:1206	Leptin resistant LiqS rats	1181:1206	Leptin resistant LiqS rats	1181:1206	Leptin resistant LiqS rats were either given free access to sucrose, had access to sucrose only at night or had access only during the day.					
36273496	7	69	contain	had	1250:1252	arg1	rats					1203:1206	Leptin resistant LiqS rats	1181:1206	Leptin resistant LiqS rats	1181:1206	Leptin resistant LiqS rats were either given free access to sucrose, had access to sucrose only at night or had access only during the day.					
36273496	7	69	contain	had	1250:1252	arg2	access					1254:1259	access	1254:1259	access to sucrose	1254:1270	Leptin resistant LiqS rats were either given free access to sucrose, had access to sucrose only at night or had access only during the day.					
36273496	3	70	from	diet	510:513	arg1	form					611:614	liquid form	604:614	liquid form	604:614	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	3	71	theme	Liquid	638:643	arg1	HSLiq					651:655	HSLiq	651:655	HSLiq	651:655	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	3	71	theme	Liquid	638:643	arg1	form					645:648	Liquid form	638:648	Liquid form (HSLiq)	638:656	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	6	72	theme	solution	1086:1093	arg1	access					1095:1100	sucrose solution access	1078:1100	sucrose solution access to either the light or dark period	1078:1135	Because LiqS rats consume sucrose throughout the day and night we tested whether limiting sucrose solution access to either the light or dark period prevented development of leptin resistance.					
36273496	6	73	theme	LiqS	996:999	arg1	rats					1001:1004	LiqS rats	996:1004	LiqS rats	996:1004	Because LiqS rats consume sucrose throughout the day and night we tested whether limiting sucrose solution access to either the light or dark period prevented development of leptin resistance.					
36273496	9	74	theme	body	1588:1591	arg1	mass					1597:1600	body fat mass	1588:1600	body fat mass	1588:1600	The results of this study show that leptin resistance of LiqS rats is independent of when the sucrose is consumed and is unrelated to total energy intake, body fat mass or serum leptin concentration.					
36273496	3	75	from	diet	554:557	arg1	form					611:614	liquid form	604:614	liquid form	604:614	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	1	76	dep	diet	218:221	arg1	addition					181:188	addition	181:188	addition	181:188	Rats offered free access to sucrose solution in addition to a sucrose-free composite diet develop leptin resistance whereas those consuming a similar amount of sucrose from a dry diet remain leptin responsive.					
36273496	3	77	theme	high	527:530	arg1	HS					546:547	HS	546:547	HS	546:547	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	3	77	theme	high	527:530	arg1	diet					540:543	a dry high sucrose diet	521:543	a dry high sucrose diet (HS)	521:548	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	3	78	theme	Dawley	470:475	arg1	rats					477:480	Female Sprague Dawley rats	455:480	Female Sprague Dawley rats	455:480	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
36273496	4	79	theme	fat	858:860	arg1	amount					843:848	the greatest amount	830:848	the greatest amount of body fat	830:860	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	4	79	theme	fat	858:860	arg1	fat					858:860	body fat	853:860	body fat	853:860	After 30 days LiqS rats were leptin resistant, but all other groups were leptin responsive even though HSLiq rats consumed as much sucrose as LiqS rats and NSLiq rats had the greatest amount of body fat.					
36273496	9	80	theme	rats	1495:1498	arg1	resistance					1476:1485	leptin resistance	1469:1485	leptin resistance of LiqS rats	1469:1498	The results of this study show that leptin resistance of LiqS rats is independent of when the sucrose is consumed and is unrelated to total energy intake, body fat mass or serum leptin concentration.					
36273496	1	81	theme	sucrose	293:299	arg1	sucrose					293:299	sucrose	293:299	sucrose	293:299	Rats offered free access to sucrose solution in addition to a sucrose-free composite diet develop leptin resistance whereas those consuming a similar amount of sucrose from a dry diet remain leptin responsive.					
36273496	1	81	theme	sucrose	293:299	arg1	amount					283:288	a similar amount	273:288	a similar amount of sucrose	273:299	Rats offered free access to sucrose solution in addition to a sucrose-free composite diet develop leptin resistance whereas those consuming a similar amount of sucrose from a dry diet remain leptin responsive.					
36273496	1	82	theme	sucrose-free	195:206	arg1	diet					218:221	a sucrose-free composite diet	193:221	a sucrose-free composite diet	193:221	Rats offered free access to sucrose solution in addition to a sucrose-free composite diet develop leptin resistance whereas those consuming a similar amount of sucrose from a dry diet remain leptin responsive.					
36273496	6	83	theme	leptin	1162:1167	arg1	resistance					1169:1178	leptin resistance	1162:1178	leptin resistance	1162:1178	Because LiqS rats consume sucrose throughout the day and night we tested whether limiting sucrose solution access to either the light or dark period prevented development of leptin resistance.					
36273496	3	84	theme	HS	627:628	arg1	diet					630:633	HS diet	627:633	HS diet	627:633	Female Sprague Dawley rats were offered a sucrose free diet (NS), a dry high sucrose diet (HS), NS diet plus 30% sucrose solution (LiqS), NS diet in liquid form (NSLiq) or HS diet in Liquid form (HSLiq).					
35713835	6	0	theme	Adsorption	1009:1018	arg1	isotherm					1020:1027	Adsorption isotherm	1009:1027	Adsorption isotherm	1009:1027	Adsorption isotherm was studied using the Langmuir, Freundlich, and D-R models.					
35713835	9	1	theme	studies	1353:1359	arg1	data					1330:1333	the data	1326:1333	the data of thermodynamics studies	1326:1359	The sorption was endothermic reaction and spontaneous, as illustrated by the data of thermodynamics studies.					
35713835	3	2	theme	Distinct	587:594	arg1	experiments					596:606	Distinct experiments	587:606	Distinct experiments	587:606	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	1	3	from	solutions	356:364	arg1	removal					312:318	removal	312:318	removal	312:318	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	1	3	from	solutions	356:364	arg1	separation					297:306	selective separation	287:306	selective separation	287:306	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	1	4	theme	thermal	214:220	arg1	technique					232:240	the thermal annealing technique	210:240	the thermal annealing technique	210:240	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	0	5	theme	lanthanum	58:66	arg1	separation					15:24	separation	15:24	separation	15:24	Extraction and separation feasibility of cerium (III) and lanthanum (III) from aqueous solution using modified graphite adsorbent.					
35713835	0	5	theme	lanthanum	58:66	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and separation feasibility of cerium (III) and lanthanum (III) from aqueous solution using modified graphite adsorbent.					
35713835	8	6	theme	200	1220:1222	arg1	capacities					1206:1215	maximum monolayer capacities	1188:1215	maximum monolayer capacities of 200 and 83.3 mg/g, respectively	1188:1250	At 25 °C, Ce(III) and La(III) have maximum monolayer capacities of 200 and 83.3 mg/g, respectively.					
35713835	2	7	dep	infrared	385:392	arg1	FTIR					395:398	FTIR	395:398	FTIR	395:398	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	7	8	used	used	1112:1115	arg2	model					1102:1106	The Langmuir model	1089:1106	The Langmuir model	1089:1106	The Langmuir model was used to better fit the results obtained.					
35713835	1	9	theme	annealing	222:230	arg1	technique					232:240	the thermal annealing technique	210:240	the thermal annealing technique	210:240	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	5	10	theme	kinetic	884:890	arg1	model					892:896	The pseudo second-order kinetic model	860:896	The pseudo second-order kinetic model	860:896	The pseudo second-order kinetic model was chosen as one which best fits the experimental evidence and better reflects the chemical sorption process.					
35713835	5	10	theme	kinetic	884:890	arg1	one					912:914	one	912:914	one	912:914	The pseudo second-order kinetic model was chosen as one which best fits the experimental evidence and better reflects the chemical sorption process.					
35713835	9	11	theme	endothermic	1270:1280	arg1	sorption					1257:1264	The sorption	1253:1264	The sorption	1253:1264	The sorption was endothermic reaction and spontaneous, as illustrated by the data of thermodynamics studies.					
35713835	9	11	theme	endothermic	1270:1280	arg1	reaction					1282:1289	endothermic reaction	1270:1289	endothermic reaction	1270:1289	The sorption was endothermic reaction and spontaneous, as illustrated by the data of thermodynamics studies.					
35713835	8	12	theme	83.3 mg/g	1228:1236	arg1	capacities					1206:1215	maximum monolayer capacities	1188:1215	maximum monolayer capacities of 200 and 83.3 mg/g, respectively	1188:1250	At 25 °C, Ce(III) and La(III) have maximum monolayer capacities of 200 and 83.3 mg/g, respectively.					
35713835	2	13	theme	surface	469:475	arg1	area					477:480	surface area	469:480	surface area	469:480	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	3	14	dep	removal	677:683	arg1	III					672:674	III	672:674	III	672:674	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	10	15	theme	adsorbent	1415:1423	arg1	material					1425:1432	a novel lanthanide adsorbent material	1396:1432	a novel lanthanide adsorbent material	1396:1432	GRA has the ability to be used as a novel lanthanide adsorbent material, especially for selective separation between Ce(III) and La(III).					
35713835	3	16	theme	pH	714:715	arg1	range					803:807	pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range	714:807	pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range	714:807	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	0	17	theme	aqueous	79:85	arg1	solution					87:94	aqueous solution	79:94	aqueous solution	79:94	Extraction and separation feasibility of cerium (III) and lanthanum (III) from aqueous solution using modified graphite adsorbent.					
35713835	1	18	dep	separation	297:306	arg1	the					283:285	the	283:285	the	283:285	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	2	19	theme	electron	506:513	arg1	SEM					527:529	SEM	527:529	SEM	527:529	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	2	19	theme	electron	506:513	arg1	microscope					515:524	scanning electron microscope	497:524	scanning electron microscope (SEM)	497:530	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	3	20	theme	shaken	718:723	arg1	time					725:728	shaken time	718:728	shaken time	718:728	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	5	21	theme	pseudo	864:869	arg1	model					892:896	The pseudo second-order kinetic model	860:896	The pseudo second-order kinetic model	860:896	The pseudo second-order kinetic model was chosen as one which best fits the experimental evidence and better reflects the chemical sorption process.					
35713835	5	21	theme	pseudo	864:869	arg1	one					912:914	one	912:914	one	912:914	The pseudo second-order kinetic model was chosen as one which best fits the experimental evidence and better reflects the chemical sorption process.					
35713835	2	22	theme	scanning	497:504	arg1	SEM					527:529	SEM	527:529	SEM	527:529	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	2	22	theme	scanning	497:504	arg1	microscope					515:524	scanning electron microscope	497:524	scanning electron microscope (SEM)	497:530	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	3	23	theme	range	803:807	arg1	effect					704:709	the effect	700:709	the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range	700:807	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	7	24	theme	Langmuir	1093:1100	arg1	model					1102:1106	The Langmuir model	1089:1106	The Langmuir model	1089:1106	The Langmuir model was used to better fit the results obtained.					
35713835	10	25	contain	has	1366:1368	arg1	GRA					1362:1364	GRA	1362:1364	GRA	1362:1364	GRA has the ability to be used as a novel lanthanide adsorbent material, especially for selective separation between Ce(III) and La(III).					
35713835	10	25	contain	has	1366:1368	arg2	ability					1374:1380	the ability to be used as a novel lanthanide adsorbent material, especially for selective separation between Ce(III) and La(III)	1370:1497	the ability to be used as a novel lanthanide adsorbent material, especially for selective separation between Ce(III) and La(III)	1370:1497	GRA has the ability to be used as a novel lanthanide adsorbent material, especially for selective separation between Ce(III) and La(III).					
35713835	1	26	used	used	246:249	arg2	GR					141:142	GR	141:142	GR	141:142	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	1	26	used	used	246:249	arg2	material					270:277	a new adsorbent material	254:277	a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions	254:364	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	1	26	used	used	246:249	arg2	Graphite					131:138	Graphite	131:138	Graphite (GR)	131:143	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	1	27	dep	Graphite	131:138	arg1	composite					173:181	composite	173:181	composite	173:181	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	1	28	theme	La	323:324	arg1	removal					312:318	removal	312:318	removal	312:318	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	1	28	theme	La	323:324	arg1	separation					297:306	selective separation	287:306	selective separation	287:306	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	2	29	dep	Fourier	367:373	arg1	transform					375:383	transform	375:383	transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM)	375:530	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	3	30	theme	different	780:788	arg1	temperatures					790:801	different temperatures	780:801	different temperatures	780:801	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	3	31	theme	initial	731:737	arg1	concentration					739:751	initial concentration	731:751	initial concentration of La(III)	731:762	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	6	32	theme	Langmuir	1051:1058	arg1	models					1081:1086	the Langmuir, Freundlich, and D-R models	1047:1086	the Langmuir, Freundlich, and D-R models	1047:1086	Adsorption isotherm was studied using the Langmuir, Freundlich, and D-R models.					
35713835	2	33	dep	DTA	433:435	arg1	TGA					438:440	TGA	438:440	TGA	438:440	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	3	34	theme	concentration	739:751	arg1	range					803:807	pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range	714:807	pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range	714:807	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	5	35	theme	experimental	936:947	arg1	evidence					949:956	the experimental evidence	932:956	the experimental evidence	932:956	The pseudo second-order kinetic model was chosen as one which best fits the experimental evidence and better reflects the chemical sorption process.					
35713835	2	36	theme	X-ray	444:448	arg1	XRD					463:465	XRD	463:465	XRD	463:465	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	2	36	theme	X-ray	444:448	arg1	diffraction					450:460	X-ray diffraction	444:460	X-ray diffraction (XRD)	444:466	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	10	37	theme	lanthanide	1404:1413	arg1	material					1425:1432	a novel lanthanide adsorbent material	1396:1432	a novel lanthanide adsorbent material	1396:1432	GRA has the ability to be used as a novel lanthanide adsorbent material, especially for selective separation between Ce(III) and La(III).					
35713835	3	38	theme	time	725:728	arg1	range					803:807	pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range	714:807	pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range	714:807	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	0	39	theme	cerium	41:46	arg1	separation					15:24	separation	15:24	separation	15:24	Extraction and separation feasibility of cerium (III) and lanthanum (III) from aqueous solution using modified graphite adsorbent.					
35713835	0	39	theme	cerium	41:46	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and separation feasibility of cerium (III) and lanthanum (III) from aqueous solution using modified graphite adsorbent.					
35713835	1	40	theme	new	256:258	arg1	material					270:277	a new adsorbent material	254:277	a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions	254:364	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	1	40	theme	new	256:258	arg1	Graphite					131:138	Graphite	131:138	Graphite (GR)	131:143	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	1	41	theme	adsorbent	260:268	arg1	material					270:277	a new adsorbent material	254:277	a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions	254:364	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	1	41	theme	adsorbent	260:268	arg1	Graphite					131:138	Graphite	131:138	Graphite (GR)	131:143	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	1	42	theme	Ce	335:336	arg1	removal					312:318	removal	312:318	removal	312:318	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	1	42	theme	Ce	335:336	arg1	separation					297:306	selective separation	287:306	selective separation	287:306	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	3	43	theme	GRA	650:652	arg1	ability					635:641	the ability	631:641	the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range	631:807	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	6	44	theme	D-R	1077:1079	arg1	models					1081:1086	the Langmuir, Freundlich, and D-R models	1047:1086	the Langmuir, Freundlich, and D-R models	1047:1086	Adsorption isotherm was studied using the Langmuir, Freundlich, and D-R models.					
35713835	8	45	theme	monolayer	1196:1204	arg1	capacities					1206:1215	maximum monolayer capacities	1188:1215	maximum monolayer capacities of 200 and 83.3 mg/g, respectively	1188:1250	At 25 °C, Ce(III) and La(III) have maximum monolayer capacities of 200 and 83.3 mg/g, respectively.					
35713835	2	46	theme	generated	567:575	arg1	material					577:584	the generated material	563:584	the generated material	563:584	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	10	47	theme	novel	1398:1402	arg1	material					1425:1432	a novel lanthanide adsorbent material	1396:1432	a novel lanthanide adsorbent material	1396:1432	GRA has the ability to be used as a novel lanthanide adsorbent material, especially for selective separation between Ce(III) and La(III).					
35713835	2	48	theme	infrared	385:392	arg1	spectroscopy					401:412	infrared (FTIR) spectroscopy	385:412	infrared (FTIR) spectroscopy	385:412	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	5	49	theme	second-order	871:882	arg1	model					892:896	The pseudo second-order kinetic model	860:896	The pseudo second-order kinetic model	860:896	The pseudo second-order kinetic model was chosen as one which best fits the experimental evidence and better reflects the chemical sorption process.					
35713835	5	49	theme	second-order	871:882	arg1	one					912:914	one	912:914	one	912:914	The pseudo second-order kinetic model was chosen as one which best fits the experimental evidence and better reflects the chemical sorption process.					
35713835	3	50	from	temperatures	790:801	arg1	Ce					769:770	Ce(III)	769:775	Ce(III) at different temperatures	769:801	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	3	50	from	temperatures	790:801	arg1	pH					714:715	pH	714:715	pH	714:715	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	3	50	from	temperatures	790:801	arg1	time					725:728	shaken time	718:728	shaken time	718:728	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	3	50	from	temperatures	790:801	arg1	concentration					739:751	initial concentration	731:751	initial concentration of La(III)	731:762	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	8	51	contain	have	1183:1186	arg1	La					1175:1176	La(III)	1175:1181	La(III)	1175:1181	At 25 °C, Ce(III) and La(III) have maximum monolayer capacities of 200 and 83.3 mg/g, respectively.					
35713835	8	51	contain	have	1183:1186	arg1	Ce					1163:1164	Ce(III)	1163:1169	Ce(III)	1163:1169	At 25 °C, Ce(III) and La(III) have maximum monolayer capacities of 200 and 83.3 mg/g, respectively.					
35713835	8	51	contain	have	1183:1186	arg2	capacities					1206:1215	maximum monolayer capacities	1188:1215	maximum monolayer capacities of 200 and 83.3 mg/g, respectively	1188:1250	At 25 °C, Ce(III) and La(III) have maximum monolayer capacities of 200 and 83.3 mg/g, respectively.					
35713835	9	52	theme	thermodynamics	1338:1351	arg1	studies					1353:1359	thermodynamics studies	1338:1359	thermodynamics studies	1338:1359	The sorption was endothermic reaction and spontaneous, as illustrated by the data of thermodynamics studies.					
35713835	10	53	theme	selective	1450:1458	arg1	separation					1460:1469	selective separation	1450:1469	selective separation between Ce(III) and La(III)	1450:1497	GRA has the ability to be used as a novel lanthanide adsorbent material, especially for selective separation between Ce(III) and La(III).					
35713835	3	54	theme	La	756:757	arg1	Ce					769:770	Ce(III)	769:775	Ce(III) at different temperatures	769:801	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	3	54	theme	La	756:757	arg1	pH					714:715	pH	714:715	pH	714:715	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	3	54	theme	La	756:757	arg1	time					725:728	shaken time	718:728	shaken time	718:728	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	3	54	theme	La	756:757	arg1	concentration					739:751	initial concentration	731:751	initial concentration of La(III)	731:762	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	2	55	used	used	542:545	arg2	Fourier					367:373	Fourier	367:373	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM)	367:530	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	5	56	theme	chemical	982:989	arg1	process					1000:1006	the chemical sorption process	978:1006	the chemical sorption process	978:1006	The pseudo second-order kinetic model was chosen as one which best fits the experimental evidence and better reflects the chemical sorption process.					
35713835	6	57	theme	Freundlich	1061:1070	arg1	models					1081:1086	the Langmuir, Freundlich, and D-R models	1047:1086	the Langmuir, Freundlich, and D-R models	1047:1086	Adsorption isotherm was studied using the Langmuir, Freundlich, and D-R models.					
35713835	8	58	theme	maximum	1188:1194	arg1	capacities					1206:1215	maximum monolayer capacities	1188:1215	maximum monolayer capacities of 200 and 83.3 mg/g, respectively	1188:1250	At 25 °C, Ce(III) and La(III) have maximum monolayer capacities of 200 and 83.3 mg/g, respectively.					
35713835	3	59	theme	Ce	669:670	arg1	removal					677:683	Ce(III) removal	669:683	Ce(III) removal	669:683	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	5	60	theme	sorption	991:998	arg1	process					1000:1006	the chemical sorption process	978:1006	the chemical sorption process	978:1006	The pseudo second-order kinetic model was chosen as one which best fits the experimental evidence and better reflects the chemical sorption process.					
35713835	1	61	theme	aqueous	348:354	arg1	solutions					356:364	aqueous solutions	348:364	aqueous solutions	348:364	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	3	62	theme	Ce	769:770	arg1	range					803:807	pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range	714:807	pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range	714:807	Distinct experiments were performed to test the ability of the GRA to La(III) and Ce(III) removal, which include the effect of pH, shaken time, initial concentration of La(III), and Ce(III) at different temperatures range.					
35713835	2	63	theme	thermal	415:421	arg1	DTA					433:435	DTA	433:435	DTA	433:435	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	2	63	theme	thermal	415:421	arg1	analysis					423:430	thermal analysis	415:430	thermal analysis (DTA, TGA)	415:441	Fourier transform infrared (FTIR) spectroscopy, thermal analysis (DTA, TGA), X-ray diffraction (XRD), surface area, porosity, and scanning electron microscope (SEM) were also used to characterize the generated material.					
35713835	1	64	theme	selective	287:295	arg1	separation					297:306	selective separation	287:306	selective separation	287:306	Graphite (GR) and graphite/alginate (GRA) composite were synthesized utilizing the thermal annealing technique and used as a new adsorbent material for the selective separation and removal of La(III) and Ce(III) from aqueous solutions.					
35713835	0	65	dep	Extraction	0:9	arg1	feasibility					26:36	feasibility	26:36	feasibility	26:36	Extraction and separation feasibility of cerium (III) and lanthanum (III) from aqueous solution using modified graphite adsorbent.					
35713835	0	66	from	solution	87:94	arg1	separation					15:24	separation	15:24	separation	15:24	Extraction and separation feasibility of cerium (III) and lanthanum (III) from aqueous solution using modified graphite adsorbent.					
35713835	0	66	from	solution	87:94	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and separation feasibility of cerium (III) and lanthanum (III) from aqueous solution using modified graphite adsorbent.					
35419690	1	0	theme	@	308:308	arg1	SD					309:310	Alg-PANI@SD	300:310	Alg-PANI@SD	300:310	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	1	0	theme	@	308:308	arg1	bio-beads					289:297	calcium-alginate bio-beads	272:297	calcium-alginate bio-beads (Alg-PANI@SD)	272:311	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	4	1	theme	entire	797:802	arg1	data					817:820	the entire experimental data	793:820	the entire experimental data	793:820	Experimental results show that the kinetic model of pseudo-first-order (PFO) and Freundlich isotherm perfectly fit the entire experimental data.					
35419690	1	2	theme	aqueous	207:213	arg1	solutions					215:223	aqueous solutions	207:223	aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD)	207:311	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	3	3	theme	ions	638:641	arg1	function					578:585	a function	576:585	a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration	576:675	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	3	3	theme	ions	638:641	arg1	tests					552:556	Batch tests	546:556	Batch tests	546:556	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	3	4	theme	time	620:623	arg1	function					578:585	a function	576:585	a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration	576:675	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	3	4	theme	time	620:623	arg1	tests					552:556	Batch tests	546:556	Batch tests	546:556	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	0	5	theme	orange	88:93	arg1	dye					97:99	orange G dye	88:99	orange G dye	88:99	Application of calcium alginate-PANI@sawdust wood hydrogel bio-beads for the removal of orange G dye from aqueous solution.					
35419690	6	6	theme	aqueous	1096:1102	arg1	media					1104:1108	aqueous media	1096:1108	aqueous media	1096:1108	The results revealed that the as-prepared Alg-PANI@SD bio-beads have the potential to be applied as a low-cost adsorbent for the adsorption of OG dye from aqueous media.					
35419690	6	7	theme	@	991:991	arg1	SD bio-beads					992:1003	the as-prepared Alg-PANI@SD bio-beads	967:1003	the as-prepared Alg-PANI@SD bio-beads	967:1003	The results revealed that the as-prepared Alg-PANI@SD bio-beads have the potential to be applied as a low-cost adsorbent for the adsorption of OG dye from aqueous media.					
35419690	3	8	theme	initial	648:654	arg1	concentration					663:675	initial OG dye concentration	648:675	initial OG dye concentration	648:675	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	4	9	theme	experimental	804:815	arg1	data					817:820	the entire experimental data	793:820	the entire experimental data	793:820	Experimental results show that the kinetic model of pseudo-first-order (PFO) and Freundlich isotherm perfectly fit the entire experimental data.					
35419690	6	10	from	media	1104:1108	arg1	adsorption					1070:1079	the adsorption	1066:1079	the adsorption of OG dye from aqueous media	1066:1108	The results revealed that the as-prepared Alg-PANI@SD bio-beads have the potential to be applied as a low-cost adsorbent for the adsorption of OG dye from aqueous media.					
35419690	1	11	theme	PANI	235:238	arg1	biocomposite					248:259	PANI@sawdust biocomposite	235:259	PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD)	235:311	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	0	12	theme	dye	97:99	arg1	removal					77:83	the removal	73:83	the removal of orange G dye from aqueous solution	73:121	Application of calcium alginate-PANI@sawdust wood hydrogel bio-beads for the removal of orange G dye from aqueous solution.					
35419690	3	13	theme	OG	656:657	arg1	concentration					663:675	initial OG dye concentration	648:675	initial OG dye concentration	648:675	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	4	14	theme	isotherm	770:777	arg1	model					721:725	the kinetic model	709:725	the kinetic model of pseudo-first-order (PFO) and Freundlich isotherm	709:777	Experimental results show that the kinetic model of pseudo-first-order (PFO) and Freundlich isotherm perfectly fit the entire experimental data.					
35419690	5	15	theme	OG	925:926	arg1	removal					932:938	OG dye removal	925:938	OG dye removal	925:938	Additionally, the prepared composite exhibited an excellent regeneration capacity and reusability for OG dye removal.					
35419690	0	16	theme	G	95:95	arg1	dye					97:99	orange G dye	88:99	orange G dye	88:99	Application of calcium alginate-PANI@sawdust wood hydrogel bio-beads for the removal of orange G dye from aqueous solution.					
35419690	2	17	used	used	498:501	arg2	adsorbent					330:338	adsorbent	330:338	adsorbent	330:338	The as-prepared adsorbent was characterized by scanning-electron-microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and Fourier transforms infrared (FT-IR) spectroscopy and used to remove orange G dye from aqueous water.					
35419690	5	18	theme	dye	928:930	arg1	removal					932:938	OG dye removal	925:938	OG dye removal	925:938	Additionally, the prepared composite exhibited an excellent regeneration capacity and reusability for OG dye removal.					
35419690	6	19	contain	have	1005:1008	arg1	SD bio-beads					992:1003	the as-prepared Alg-PANI@SD bio-beads	967:1003	the as-prepared Alg-PANI@SD bio-beads	967:1003	The results revealed that the as-prepared Alg-PANI@SD bio-beads have the potential to be applied as a low-cost adsorbent for the adsorption of OG dye from aqueous media.					
35419690	6	19	contain	have	1005:1008	arg2	potential					1014:1022	the potential to be applied as a low-cost adsorbent for the adsorption of OG dye from aqueous media	1010:1108	the potential to be applied as a low-cost adsorbent for the adsorption of OG dye from aqueous media	1010:1108	The results revealed that the as-prepared Alg-PANI@SD bio-beads have the potential to be applied as a low-cost adsorbent for the adsorption of OG dye from aqueous media.					
35419690	5	20	theme	excellent	873:881	arg1	capacity					896:903	an excellent regeneration capacity	870:903	an excellent regeneration capacity	870:903	Additionally, the prepared composite exhibited an excellent regeneration capacity and reusability for OG dye removal.					
35419690	2	21	dep	Fourier	445:451	arg1	transforms					453:462	transforms	453:462	transforms infrared (FT-IR) spectroscopy	453:492	The as-prepared adsorbent was characterized by scanning-electron-microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and Fourier transforms infrared (FT-IR) spectroscopy and used to remove orange G dye from aqueous water.					
35419690	1	22	theme	adsorption	158:167	arg1	performance					169:179	the adsorption performance	154:179	the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD)	154:311	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	6	23	theme	as-prepared	971:981	arg1	SD bio-beads					992:1003	the as-prepared Alg-PANI@SD bio-beads	967:1003	the as-prepared Alg-PANI@SD bio-beads	967:1003	The results revealed that the as-prepared Alg-PANI@SD bio-beads have the potential to be applied as a low-cost adsorbent for the adsorption of OG dye from aqueous media.					
35419690	1	24	theme	@	239:239	arg1	biocomposite					248:259	PANI@sawdust biocomposite	235:259	PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD)	235:311	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	5	25	theme	regeneration	883:894	arg1	capacity					896:903	an excellent regeneration capacity	870:903	an excellent regeneration capacity	870:903	Additionally, the prepared composite exhibited an excellent regeneration capacity and reusability for OG dye removal.					
35419690	0	26	theme	alginate-PANI	23:35	arg1	hydrogel					50:57	calcium alginate-PANI@sawdust wood hydrogel	15:57	calcium alginate-PANI@sawdust wood hydrogel	15:57	Application of calcium alginate-PANI@sawdust wood hydrogel bio-beads for the removal of orange G dye from aqueous solution.					
35419690	1	27	theme	sawdust	240:246	arg1	biocomposite					248:259	PANI@sawdust biocomposite	235:259	PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD)	235:311	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	0	28	theme	aqueous	106:112	arg1	solution					114:121	aqueous solution	106:121	aqueous solution	106:121	Application of calcium alginate-PANI@sawdust wood hydrogel bio-beads for the removal of orange G dye from aqueous solution.					
35419690	4	29	theme	Experimental	678:689	arg1	results					691:697	Experimental results	678:697	Experimental results	678:697	Experimental results show that the kinetic model of pseudo-first-order (PFO) and Freundlich isotherm perfectly fit the entire experimental data.					
35419690	0	30	theme	calcium	15:21	arg1	hydrogel					50:57	calcium alginate-PANI@sawdust wood hydrogel	15:57	calcium alginate-PANI@sawdust wood hydrogel	15:57	Application of calcium alginate-PANI@sawdust wood hydrogel bio-beads for the removal of orange G dye from aqueous solution.					
35419690	3	31	theme	dye	659:661	arg1	concentration					663:675	initial OG dye concentration	648:675	initial OG dye concentration	648:675	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	3	32	theme	adsorbent	590:598	arg1	dosage					600:605	adsorbent dosage	590:605	adsorbent dosage	590:605	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	6	33	theme	Alg-PANI	983:990	arg1	SD bio-beads					992:1003	the as-prepared Alg-PANI@SD bio-beads	967:1003	the as-prepared Alg-PANI@SD bio-beads	967:1003	The results revealed that the as-prepared Alg-PANI@SD bio-beads have the potential to be applied as a low-cost adsorbent for the adsorption of OG dye from aqueous media.					
35419690	1	34	theme	orange	184:189	arg1	dye					198:200	orange G (OG) dye	184:200	orange G (OG) dye	184:200	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	0	35	theme	sawdust	37:43	arg1	hydrogel					50:57	calcium alginate-PANI@sawdust wood hydrogel	15:57	calcium alginate-PANI@sawdust wood hydrogel	15:57	Application of calcium alginate-PANI@sawdust wood hydrogel bio-beads for the removal of orange G dye from aqueous solution.					
35419690	3	36	theme	concentration	663:675	arg1	function					578:585	a function	576:585	a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration	576:675	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	3	36	theme	concentration	663:675	arg1	tests					552:556	Batch tests	546:556	Batch tests	546:556	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	3	37	theme	dosage	600:605	arg1	function					578:585	a function	576:585	a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration	576:675	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	3	37	theme	dosage	600:605	arg1	tests					552:556	Batch tests	546:556	Batch tests	546:556	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	3	38	theme	Batch	546:550	arg1	function					578:585	a function	576:585	a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration	576:675	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	3	38	theme	Batch	546:550	arg1	tests					552:556	Batch tests	546:556	Batch tests	546:556	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	1	39	theme	G	191:191	arg1	dye					198:200	orange G (OG) dye	184:200	orange G (OG) dye	184:200	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	0	40	theme	@	36:36	arg1	hydrogel					50:57	calcium alginate-PANI@sawdust wood hydrogel	15:57	calcium alginate-PANI@sawdust wood hydrogel	15:57	Application of calcium alginate-PANI@sawdust wood hydrogel bio-beads for the removal of orange G dye from aqueous solution.					
35419690	1	41	from	solutions	215:223	arg1	performance					169:179	the adsorption performance	154:179	the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD)	154:311	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	2	42	theme	energy-dispersive	403:419	arg1	spectroscopy					421:432	X-ray energy-dispersive spectroscopy	397:432	X-ray energy-dispersive spectroscopy (EDS)	397:438	The as-prepared adsorbent was characterized by scanning-electron-microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and Fourier transforms infrared (FT-IR) spectroscopy and used to remove orange G dye from aqueous water.					
35419690	2	42	theme	energy-dispersive	403:419	arg1	EDS					435:437	EDS	435:437	EDS	435:437	The as-prepared adsorbent was characterized by scanning-electron-microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and Fourier transforms infrared (FT-IR) spectroscopy and used to remove orange G dye from aqueous water.					
35419690	2	43	theme	FT-IR	474:478	arg1	spectroscopy					481:492	infrared (FT-IR) spectroscopy	464:492	infrared (FT-IR) spectroscopy	464:492	The as-prepared adsorbent was characterized by scanning-electron-microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and Fourier transforms infrared (FT-IR) spectroscopy and used to remove orange G dye from aqueous water.					
35419690	0	44	theme	hydrogel	50:57	arg1	Application					0:10	Application	0:10	Application of calcium alginate-PANI@sawdust wood hydrogel	0:57	Application of calcium alginate-PANI@sawdust wood hydrogel bio-beads for the removal of orange G dye from aqueous solution.					
35419690	1	45	theme	calcium-alginate	272:287	arg1	SD					309:310	Alg-PANI@SD	300:310	Alg-PANI@SD	300:310	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	1	45	theme	calcium-alginate	272:287	arg1	bio-beads					289:297	calcium-alginate bio-beads	272:297	calcium-alginate bio-beads (Alg-PANI@SD)	272:311	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	2	46	theme	aqueous	531:537	arg1	water					539:543	aqueous water	531:543	aqueous water	531:543	The as-prepared adsorbent was characterized by scanning-electron-microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and Fourier transforms infrared (FT-IR) spectroscopy and used to remove orange G dye from aqueous water.					
35419690	5	47	theme	prepared	841:848	arg1	composite					850:858	the prepared composite	837:858	the prepared composite	837:858	Additionally, the prepared composite exhibited an excellent regeneration capacity and reusability for OG dye removal.					
35419690	0	48	theme	wood	45:48	arg1	hydrogel					50:57	calcium alginate-PANI@sawdust wood hydrogel	15:57	calcium alginate-PANI@sawdust wood hydrogel	15:57	Application of calcium alginate-PANI@sawdust wood hydrogel bio-beads for the removal of orange G dye from aqueous solution.					
35419690	2	49	theme	orange	513:518	arg1	dye					522:524	orange G dye	513:524	orange G dye	513:524	The as-prepared adsorbent was characterized by scanning-electron-microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and Fourier transforms infrared (FT-IR) spectroscopy and used to remove orange G dye from aqueous water.					
35419690	4	50	theme	Freundlich	759:768	arg1	isotherm					770:777	Freundlich isotherm	759:777	Freundlich isotherm	759:777	Experimental results show that the kinetic model of pseudo-first-order (PFO) and Freundlich isotherm perfectly fit the entire experimental data.					
35419690	3	51	theme	interfering	626:636	arg1	ions					638:641	interfering ions	626:641	interfering ions	626:641	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	3	52	theme	contact	612:618	arg1	time					620:623	contact time	612:623	contact time	612:623	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	6	53	theme	OG	1084:1085	arg1	dye					1087:1089	OG dye	1084:1089	OG dye	1084:1089	The results revealed that the as-prepared Alg-PANI@SD bio-beads have the potential to be applied as a low-cost adsorbent for the adsorption of OG dye from aqueous media.					
35419690	1	54	theme	OG	194:195	arg1	dye					198:200	orange G (OG) dye	184:200	orange G (OG) dye	184:200	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	2	55	theme	G	520:520	arg1	dye					522:524	orange G dye	513:524	orange G dye	513:524	The as-prepared adsorbent was characterized by scanning-electron-microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and Fourier transforms infrared (FT-IR) spectroscopy and used to remove orange G dye from aqueous water.					
35419690	4	56	theme	kinetic	713:719	arg1	model					721:725	the kinetic model	709:725	the kinetic model of pseudo-first-order (PFO) and Freundlich isotherm	709:777	Experimental results show that the kinetic model of pseudo-first-order (PFO) and Freundlich isotherm perfectly fit the entire experimental data.					
35419690	0	57	from	solution	114:121	arg1	removal					77:83	the removal	73:83	the removal of orange G dye from aqueous solution	73:121	Application of calcium alginate-PANI@sawdust wood hydrogel bio-beads for the removal of orange G dye from aqueous solution.					
35419690	3	58	theme	pH	608:609	arg1	function					578:585	a function	576:585	a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration	576:675	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	3	58	theme	pH	608:609	arg1	tests					552:556	Batch tests	546:556	Batch tests	546:556	Batch tests were performed as a function of adsorbent dosage, pH, contact time, interfering ions, and initial OG dye concentration.					
35419690	4	59	theme	pseudo-first-order	730:747	arg1	model					721:725	the kinetic model	709:725	the kinetic model of pseudo-first-order (PFO) and Freundlich isotherm	709:777	Experimental results show that the kinetic model of pseudo-first-order (PFO) and Freundlich isotherm perfectly fit the entire experimental data.					
35419690	1	60	theme	dye	198:200	arg1	performance					169:179	the adsorption performance	154:179	the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD)	154:311	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	2	61	theme	infrared	464:471	arg1	spectroscopy					481:492	infrared (FT-IR) spectroscopy	464:492	infrared (FT-IR) spectroscopy	464:492	The as-prepared adsorbent was characterized by scanning-electron-microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and Fourier transforms infrared (FT-IR) spectroscopy and used to remove orange G dye from aqueous water.					
35419690	1	62	theme	Alg-PANI	300:307	arg1	SD					309:310	Alg-PANI@SD	300:310	Alg-PANI@SD	300:310	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	1	62	theme	Alg-PANI	300:307	arg1	bio-beads					289:297	calcium-alginate bio-beads	272:297	calcium-alginate bio-beads (Alg-PANI@SD)	272:311	This work aims to investigate the adsorption performance of orange G (OG) dye from aqueous solutions employing PANI@sawdust biocomposite enrobed by calcium-alginate bio-beads (Alg-PANI@SD).					
35419690	2	63	theme	X-ray	397:401	arg1	spectroscopy					421:432	X-ray energy-dispersive spectroscopy	397:432	X-ray energy-dispersive spectroscopy (EDS)	397:438	The as-prepared adsorbent was characterized by scanning-electron-microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and Fourier transforms infrared (FT-IR) spectroscopy and used to remove orange G dye from aqueous water.					
35419690	2	63	theme	X-ray	397:401	arg1	EDS					435:437	EDS	435:437	EDS	435:437	The as-prepared adsorbent was characterized by scanning-electron-microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and Fourier transforms infrared (FT-IR) spectroscopy and used to remove orange G dye from aqueous water.					
35419690	6	64	theme	dye	1087:1089	arg1	adsorption					1070:1079	the adsorption	1066:1079	the adsorption of OG dye from aqueous media	1066:1108	The results revealed that the as-prepared Alg-PANI@SD bio-beads have the potential to be applied as a low-cost adsorbent for the adsorption of OG dye from aqueous media.					
37295195	3	0	theme	study	558:562	arg1	purpose					542:548	The purpose	538:548	The purpose of this study	538:562	The purpose of this study was to construct two antibacterial coatings with 5 or 10 sodium alginate/chlorhexidine bilayers on titanium surfaces using layer-by-layer self-assembly technology to promote soft-tissue sealing.					
37295195	8	1	with	integration	1721:1731	arg1	devices					1746:1752	implant devices	1738:1752	implant devices	1738:1752	Therefore, this study demonstrated that the multilayered coating could prevent implant-related infections in the initial stage of implant surgery and then improve soft-tissue integration with implant devices.					
37295195	4	2	theme	titanium	969:976	arg1	surface					978:984	the porous titanium surface	958:984	the porous titanium surface	958:984	The corresponding chemical composition, surface topography, wettability and release behaviour were investigated to prove that the resultant coating of sodium alginate and chlorhexidine was coated on the porous titanium surface.					
37295195	5	3	theme	antibacterial	1008:1020	arg1	results					1022:1028	In-vitro and in-vivo antibacterial results	987:1028	In-vitro and in-vivo antibacterial results	987:1028	In-vitro and in-vivo antibacterial results showed that both prepared coatings inhibited or killed the bacteria on their surfaces and the surrounding areas to prevent plaque biofilm formation, especially the coating with 10 bilayers.					
37295195	2	4	theme	implant	394:400	arg1	surface					402:408	the implant surface	390:408	the implant surface	390:408	However, the colonization of oral pathogens on the implant surface and surrounding soft tissues can disturb the early establishment of soft-tissue sealing and even induce peri-implant infection.					
37295195	8	5	theme	soft-tissue	1709:1719	arg1	integration					1721:1731	soft-tissue integration	1709:1731	soft-tissue integration with implant devices	1709:1752	Therefore, this study demonstrated that the multilayered coating could prevent implant-related infections in the initial stage of implant surgery and then improve soft-tissue integration with implant devices.					
37295195	4	6	theme	resultant	889:897	arg1	coating					899:905	the resultant coating	885:905	the resultant coating of sodium alginate and chlorhexidine	885:942	The corresponding chemical composition, surface topography, wettability and release behaviour were investigated to prove that the resultant coating of sodium alginate and chlorhexidine was coated on the porous titanium surface.					
37295195	4	7	theme	chemical	777:784	arg1	composition					786:796	The corresponding chemical composition	759:796	The corresponding chemical composition	759:796	The corresponding chemical composition, surface topography, wettability and release behaviour were investigated to prove that the resultant coating of sodium alginate and chlorhexidine was coated on the porous titanium surface.					
37295195	5	8	theme	in-vivo	1000:1006	arg1	results					1022:1028	In-vitro and in-vivo antibacterial results	987:1028	In-vitro and in-vivo antibacterial results	987:1028	In-vitro and in-vivo antibacterial results showed that both prepared coatings inhibited or killed the bacteria on their surfaces and the surrounding areas to prevent plaque biofilm formation, especially the coating with 10 bilayers.					
37295195	2	9	from	colonization	356:367	arg1	surface					402:408	the implant surface	390:408	the implant surface	390:408	However, the colonization of oral pathogens on the implant surface and surrounding soft tissues can disturb the early establishment of soft-tissue sealing and even induce peri-implant infection.					
37295195	2	9	from	colonization	356:367	arg1	tissues					431:437	surrounding soft tissues	414:437	surrounding soft tissues	414:437	However, the colonization of oral pathogens on the implant surface and surrounding soft tissues can disturb the early establishment of soft-tissue sealing and even induce peri-implant infection.					
37295195	8	10	theme	implant	1738:1744	arg1	devices					1746:1752	implant devices	1738:1752	implant devices	1738:1752	Therefore, this study demonstrated that the multilayered coating could prevent implant-related infections in the initial stage of implant surgery and then improve soft-tissue integration with implant devices.					
37295195	4	11	theme	surface	799:805	arg1	topography					807:816	surface topography	799:816	surface topography	799:816	The corresponding chemical composition, surface topography, wettability and release behaviour were investigated to prove that the resultant coating of sodium alginate and chlorhexidine was coated on the porous titanium surface.					
37295195	5	12	theme	prepared	1047:1054	arg1	coatings					1056:1063	both prepared coatings	1042:1063	both prepared coatings	1042:1063	In-vitro and in-vivo antibacterial results showed that both prepared coatings inhibited or killed the bacteria on their surfaces and the surrounding areas to prevent plaque biofilm formation, especially the coating with 10 bilayers.					
37295195	4	13	theme	release	835:841	arg1	behaviour					843:851	release behaviour	835:851	release behaviour	835:851	The corresponding chemical composition, surface topography, wettability and release behaviour were investigated to prove that the resultant coating of sodium alginate and chlorhexidine was coated on the porous titanium surface.					
37295195	8	14	theme	implant	1676:1682	arg1	surgery					1684:1690	implant surgery	1676:1690	implant surgery	1676:1690	Therefore, this study demonstrated that the multilayered coating could prevent implant-related infections in the initial stage of implant surgery and then improve soft-tissue integration with implant devices.					
37295195	7	15	theme	inflammation	1524:1535	arg1	in-vivo					1537:1543	bacteria-induced subcutaneous inflammation in-vivo	1494:1543	bacteria-induced subcutaneous inflammation in-vivo	1494:1543	More importantly, both coatings achieved cell adhesion and proliferation in an in-vitro bacterial environment and effectively alleviated bacteria-induced subcutaneous inflammation in-vivo.					
37295195	7	16	theme	bacteria-induced	1494:1509	arg1	in-vivo					1537:1543	bacteria-induced subcutaneous inflammation in-vivo	1494:1543	bacteria-induced subcutaneous inflammation in-vivo	1494:1543	More importantly, both coatings achieved cell adhesion and proliferation in an in-vitro bacterial environment and effectively alleviated bacteria-induced subcutaneous inflammation in-vivo.					
37295195	3	17	theme	antibacterial	585:597	arg1	coatings					599:606	two antibacterial coatings	581:606	two antibacterial coatings with 5 or 10 sodium alginate/chlorhexidine bilayers	581:658	The purpose of this study was to construct two antibacterial coatings with 5 or 10 sodium alginate/chlorhexidine bilayers on titanium surfaces using layer-by-layer self-assembly technology to promote soft-tissue sealing.					
37295195	7	18	theme	subcutaneous	1511:1522	arg1	in-vivo					1537:1543	bacteria-induced subcutaneous inflammation in-vivo	1494:1543	bacteria-induced subcutaneous inflammation in-vivo	1494:1543	More importantly, both coatings achieved cell adhesion and proliferation in an in-vitro bacterial environment and effectively alleviated bacteria-induced subcutaneous inflammation in-vivo.					
37295195	0	19	theme	sodium	9:14	arg1	coating					50:56	Built-up sodium alginate/chlorhexidine multilayer coating	0:56	Built-up sodium alginate/chlorhexidine multilayer coating on dental implants with initiating anti-infection and cyto-compatibility	0:129	Built-up sodium alginate/chlorhexidine multilayer coating on dental implants with initiating anti-infection and cyto-compatibility sequentially for soft-tissue sealing.					
37295195	0	20	from	coating	50:56	arg1	implants					68:75	dental implants	61:75	dental implants	61:75	Built-up sodium alginate/chlorhexidine multilayer coating on dental implants with initiating anti-infection and cyto-compatibility sequentially for soft-tissue sealing.					
37295195	4	21	theme	porous	962:967	arg1	surface					978:984	the porous titanium surface	958:984	the porous titanium surface	958:984	The corresponding chemical composition, surface topography, wettability and release behaviour were investigated to prove that the resultant coating of sodium alginate and chlorhexidine was coated on the porous titanium surface.					
37295195	7	22	theme	cell	1398:1401	arg1	adhesion					1403:1410	cell adhesion	1398:1410	cell adhesion	1398:1410	More importantly, both coatings achieved cell adhesion and proliferation in an in-vitro bacterial environment and effectively alleviated bacteria-induced subcutaneous inflammation in-vivo.					
37295195	8	23	theme	implant-related	1625:1639	arg1	infections					1641:1650	implant-related infections	1625:1650	implant-related infections in the initial stage of implant surgery	1625:1690	Therefore, this study demonstrated that the multilayered coating could prevent implant-related infections in the initial stage of implant surgery and then improve soft-tissue integration with implant devices.					
37295195	4	24	theme	corresponding	763:775	arg1	composition					786:796	The corresponding chemical composition	759:796	The corresponding chemical composition	759:796	The corresponding chemical composition, surface topography, wettability and release behaviour were investigated to prove that the resultant coating of sodium alginate and chlorhexidine was coated on the porous titanium surface.					
37295195	0	25	theme	Built-up	0:7	arg1	coating					50:56	Built-up sodium alginate/chlorhexidine multilayer coating	0:56	Built-up sodium alginate/chlorhexidine multilayer coating on dental implants with initiating anti-infection and cyto-compatibility	0:129	Built-up sodium alginate/chlorhexidine multilayer coating on dental implants with initiating anti-infection and cyto-compatibility sequentially for soft-tissue sealing.					
37295195	1	26	theme	dental	326:331	arg1	implants					333:340	dental implants	326:340	dental implants	326:340	Soft-tissue sealing at transmucosal sites is very important for preventing the invasion of pathogens and maintaining the long-term stability and function of dental implants.					
37295195	8	27	theme	surgery	1684:1690	arg1	stage					1667:1671	the initial stage	1655:1671	the initial stage of implant surgery	1655:1690	Therefore, this study demonstrated that the multilayered coating could prevent implant-related infections in the initial stage of implant surgery and then improve soft-tissue integration with implant devices.					
37295195	3	28	theme	sodium	621:626	arg1	bilayers					651:658	5 or 10 sodium alginate/chlorhexidine bilayers	613:658	5 or 10 sodium alginate/chlorhexidine bilayers	613:658	The purpose of this study was to construct two antibacterial coatings with 5 or 10 sodium alginate/chlorhexidine bilayers on titanium surfaces using layer-by-layer self-assembly technology to promote soft-tissue sealing.					
37295195	8	29	from	infections	1641:1650	arg1	stage					1667:1671	the initial stage	1655:1671	the initial stage of implant surgery	1655:1690	Therefore, this study demonstrated that the multilayered coating could prevent implant-related infections in the initial stage of implant surgery and then improve soft-tissue integration with implant devices.					
37295195	2	30	theme	soft	426:429	arg1	tissues					431:437	surrounding soft tissues	414:437	surrounding soft tissues	414:437	However, the colonization of oral pathogens on the implant surface and surrounding soft tissues can disturb the early establishment of soft-tissue sealing and even induce peri-implant infection.					
37295195	0	31	theme	multilayer	39:48	arg1	coating					50:56	Built-up sodium alginate/chlorhexidine multilayer coating	0:56	Built-up sodium alginate/chlorhexidine multilayer coating on dental implants with initiating anti-infection and cyto-compatibility	0:129	Built-up sodium alginate/chlorhexidine multilayer coating on dental implants with initiating anti-infection and cyto-compatibility sequentially for soft-tissue sealing.					
37295195	1	32	theme	implants	333:340	arg1	function					314:321	function	314:321	function	314:321	Soft-tissue sealing at transmucosal sites is very important for preventing the invasion of pathogens and maintaining the long-term stability and function of dental implants.					
37295195	1	32	theme	implants	333:340	arg1	stability					300:308	long-term stability	290:308	long-term stability	290:308	Soft-tissue sealing at transmucosal sites is very important for preventing the invasion of pathogens and maintaining the long-term stability and function of dental implants.					
37295195	5	33	theme	plaque	1153:1158	arg1	formation					1168:1176	plaque biofilm formation	1153:1176	plaque biofilm formation	1153:1176	In-vitro and in-vivo antibacterial results showed that both prepared coatings inhibited or killed the bacteria on their surfaces and the surrounding areas to prevent plaque biofilm formation, especially the coating with 10 bilayers.					
37295195	2	34	theme	soft-tissue	478:488	arg1	establishment					461:473	the early establishment	451:473	the early establishment of soft-tissue sealing	451:496	However, the colonization of oral pathogens on the implant surface and surrounding soft tissues can disturb the early establishment of soft-tissue sealing and even induce peri-implant infection.					
37295195	5	35	with	coating	1194:1200	arg1	bilayers					1210:1217	10 bilayers	1207:1217	10 bilayers	1207:1217	In-vitro and in-vivo antibacterial results showed that both prepared coatings inhibited or killed the bacteria on their surfaces and the surrounding areas to prevent plaque biofilm formation, especially the coating with 10 bilayers.					
37295195	6	36	theme	fibroblasts	1277:1287	arg1	adhesion					1265:1272	the initial adhesion	1253:1272	the initial adhesion of fibroblasts	1253:1287	Although both coatings inhibited the initial adhesion of fibroblasts, the cytocompatibility gradually improved with coating degradation.					
37295195	7	37	theme	bacterial	1445:1453	arg1	environment					1455:1465	an in-vitro bacterial environment	1433:1465	an in-vitro bacterial environment	1433:1465	More importantly, both coatings achieved cell adhesion and proliferation in an in-vitro bacterial environment and effectively alleviated bacteria-induced subcutaneous inflammation in-vivo.					
37295195	7	38	theme	in-vitro	1436:1443	arg1	environment					1455:1465	an in-vitro bacterial environment	1433:1465	an in-vitro bacterial environment	1433:1465	More importantly, both coatings achieved cell adhesion and proliferation in an in-vitro bacterial environment and effectively alleviated bacteria-induced subcutaneous inflammation in-vivo.					
37295195	5	39	theme	In-vitro	987:994	arg1	results					1022:1028	In-vitro and in-vivo antibacterial results	987:1028	In-vitro and in-vivo antibacterial results	987:1028	In-vitro and in-vivo antibacterial results showed that both prepared coatings inhibited or killed the bacteria on their surfaces and the surrounding areas to prevent plaque biofilm formation, especially the coating with 10 bilayers.					
37295195	2	40	theme	early	455:459	arg1	establishment					461:473	the early establishment	451:473	the early establishment of soft-tissue sealing	451:496	However, the colonization of oral pathogens on the implant surface and surrounding soft tissues can disturb the early establishment of soft-tissue sealing and even induce peri-implant infection.					
37295195	8	41	theme	multilayered	1590:1601	arg1	coating					1603:1609	the multilayered coating	1586:1609	the multilayered coating	1586:1609	Therefore, this study demonstrated that the multilayered coating could prevent implant-related infections in the initial stage of implant surgery and then improve soft-tissue integration with implant devices.					
37295195	3	42	theme	titanium	663:670	arg1	surfaces					672:679	titanium surfaces	663:679	titanium surfaces using layer-by-layer self-assembly technology to promote soft-tissue sealing	663:756	The purpose of this study was to construct two antibacterial coatings with 5 or 10 sodium alginate/chlorhexidine bilayers on titanium surfaces using layer-by-layer self-assembly technology to promote soft-tissue sealing.					
37295195	1	43	theme	pathogens	260:268	arg1	invasion					248:255	the invasion	244:255	the invasion of pathogens	244:268	Soft-tissue sealing at transmucosal sites is very important for preventing the invasion of pathogens and maintaining the long-term stability and function of dental implants.					
37295195	2	44	theme	peri-implant	514:525	arg1	infection					527:535	peri-implant infection	514:535	peri-implant infection	514:535	However, the colonization of oral pathogens on the implant surface and surrounding soft tissues can disturb the early establishment of soft-tissue sealing and even induce peri-implant infection.					
37295195	8	45	theme	initial	1659:1665	arg1	stage					1667:1671	the initial stage	1655:1671	the initial stage of implant surgery	1655:1690	Therefore, this study demonstrated that the multilayered coating could prevent implant-related infections in the initial stage of implant surgery and then improve soft-tissue integration with implant devices.					
37295195	3	46	theme	alginate/chlorhexidine	628:649	arg1	bilayers					651:658	5 or 10 sodium alginate/chlorhexidine bilayers	613:658	5 or 10 sodium alginate/chlorhexidine bilayers	613:658	The purpose of this study was to construct two antibacterial coatings with 5 or 10 sodium alginate/chlorhexidine bilayers on titanium surfaces using layer-by-layer self-assembly technology to promote soft-tissue sealing.					
37295195	6	47	theme	initial	1257:1263	arg1	adhesion					1265:1272	the initial adhesion	1253:1272	the initial adhesion of fibroblasts	1253:1287	Although both coatings inhibited the initial adhesion of fibroblasts, the cytocompatibility gradually improved with coating degradation.					
37295195	0	48	theme	dental	61:66	arg1	implants					68:75	dental implants	61:75	dental implants	61:75	Built-up sodium alginate/chlorhexidine multilayer coating on dental implants with initiating anti-infection and cyto-compatibility sequentially for soft-tissue sealing.					
37295195	5	49	theme	biofilm	1160:1166	arg1	formation					1168:1176	plaque biofilm formation	1153:1176	plaque biofilm formation	1153:1176	In-vitro and in-vivo antibacterial results showed that both prepared coatings inhibited or killed the bacteria on their surfaces and the surrounding areas to prevent plaque biofilm formation, especially the coating with 10 bilayers.					
37295195	4	50	theme	chlorhexidine	930:942	arg1	coating					899:905	the resultant coating	885:905	the resultant coating of sodium alginate and chlorhexidine	885:942	The corresponding chemical composition, surface topography, wettability and release behaviour were investigated to prove that the resultant coating of sodium alginate and chlorhexidine was coated on the porous titanium surface.					
37295195	5	51	theme	surrounding	1124:1134	arg1	areas					1136:1140	the surrounding areas	1120:1140	the surrounding areas	1120:1140	In-vitro and in-vivo antibacterial results showed that both prepared coatings inhibited or killed the bacteria on their surfaces and the surrounding areas to prevent plaque biofilm formation, especially the coating with 10 bilayers.					
37295195	3	52	theme	layer-by-layer	687:700	arg1	technology					716:725	layer-by-layer self-assembly technology	687:725	layer-by-layer self-assembly technology	687:725	The purpose of this study was to construct two antibacterial coatings with 5 or 10 sodium alginate/chlorhexidine bilayers on titanium surfaces using layer-by-layer self-assembly technology to promote soft-tissue sealing.					
37295195	4	53	theme	alginate	917:924	arg1	coating					899:905	the resultant coating	885:905	the resultant coating of sodium alginate and chlorhexidine	885:942	The corresponding chemical composition, surface topography, wettability and release behaviour were investigated to prove that the resultant coating of sodium alginate and chlorhexidine was coated on the porous titanium surface.					
37295195	2	54	theme	pathogens	377:385	arg1	colonization					356:367	the colonization	352:367	the colonization of oral pathogens on the implant surface and surrounding soft tissues	352:437	However, the colonization of oral pathogens on the implant surface and surrounding soft tissues can disturb the early establishment of soft-tissue sealing and even induce peri-implant infection.					
37295195	1	55	dep	stability	300:308	arg1	the					286:288	the	286:288	the	286:288	Soft-tissue sealing at transmucosal sites is very important for preventing the invasion of pathogens and maintaining the long-term stability and function of dental implants.					
37295195	3	56	theme	self-assembly	702:714	arg1	technology					716:725	layer-by-layer self-assembly technology	687:725	layer-by-layer self-assembly technology	687:725	The purpose of this study was to construct two antibacterial coatings with 5 or 10 sodium alginate/chlorhexidine bilayers on titanium surfaces using layer-by-layer self-assembly technology to promote soft-tissue sealing.					
37295195	2	57	theme	oral	372:375	arg1	pathogens					377:385	oral pathogens	372:385	oral pathogens	372:385	However, the colonization of oral pathogens on the implant surface and surrounding soft tissues can disturb the early establishment of soft-tissue sealing and even induce peri-implant infection.					
37295195	5	58	from	bacteria	1089:1096	arg1	surfaces					1107:1114	their surfaces	1101:1114	their surfaces	1101:1114	In-vitro and in-vivo antibacterial results showed that both prepared coatings inhibited or killed the bacteria on their surfaces and the surrounding areas to prevent plaque biofilm formation, especially the coating with 10 bilayers.					
37295195	5	58	from	bacteria	1089:1096	arg1	areas					1136:1140	the surrounding areas	1120:1140	the surrounding areas	1120:1140	In-vitro and in-vivo antibacterial results showed that both prepared coatings inhibited or killed the bacteria on their surfaces and the surrounding areas to prevent plaque biofilm formation, especially the coating with 10 bilayers.					
37295195	4	59	theme	sodium	910:915	arg1	alginate					917:924	sodium alginate	910:924	sodium alginate	910:924	The corresponding chemical composition, surface topography, wettability and release behaviour were investigated to prove that the resultant coating of sodium alginate and chlorhexidine was coated on the porous titanium surface.					
37295195	2	60	theme	surrounding	414:424	arg1	tissues					431:437	surrounding soft tissues	414:437	surrounding soft tissues	414:437	However, the colonization of oral pathogens on the implant surface and surrounding soft tissues can disturb the early establishment of soft-tissue sealing and even induce peri-implant infection.					
37295195	3	61	with	coatings	599:606	arg1	bilayers					651:658	5 or 10 sodium alginate/chlorhexidine bilayers	613:658	5 or 10 sodium alginate/chlorhexidine bilayers	613:658	The purpose of this study was to construct two antibacterial coatings with 5 or 10 sodium alginate/chlorhexidine bilayers on titanium surfaces using layer-by-layer self-assembly technology to promote soft-tissue sealing.					
37295195	0	62	theme	alginate/chlorhexidine	16:37	arg1	coating					50:56	Built-up sodium alginate/chlorhexidine multilayer coating	0:56	Built-up sodium alginate/chlorhexidine multilayer coating on dental implants with initiating anti-infection and cyto-compatibility	0:129	Built-up sodium alginate/chlorhexidine multilayer coating on dental implants with initiating anti-infection and cyto-compatibility sequentially for soft-tissue sealing.					
37295195	1	63	theme	transmucosal	192:203	arg1	sites					205:209	transmucosal sites	192:209	transmucosal sites	192:209	Soft-tissue sealing at transmucosal sites is very important for preventing the invasion of pathogens and maintaining the long-term stability and function of dental implants.					
37295195	1	64	theme	long-term	290:298	arg1	stability					300:308	long-term stability	290:308	long-term stability	290:308	Soft-tissue sealing at transmucosal sites is very important for preventing the invasion of pathogens and maintaining the long-term stability and function of dental implants.					
35616239	11	0	theme	application	2117:2127	arg1	prospects					2129:2137	good application prospects	2112:2137	good application prospects	2112:2137	The results indicated that the material has good application prospects in the analysis of protein phosphorylation.					
35616239	12	1	theme	synthesis	2267:2275	arg1	process					2277:2283	its synthesis process	2263:2283	its synthesis process	2263:2283	Furthermore, TiO2-CM consists of green and cheap cellulose as the skeleton, and its synthesis process is environment-friendly, simple, and inexpensive.					
35616239	6	2	theme	peptides	1280:1287	arg1	loss					1272:1275	the loss	1268:1275	the loss of peptides and materials	1268:1301	The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials.					
35616239	7	3	theme	surface	1448:1454	arg1	area					1456:1459	a large surface area	1440:1459	a large surface area	1440:1459	Moreover, a large amount of TiO2 was introduced onto the surface of the CM material, and thus, the resultant TiO2-CM material exhibited a large surface area.					
35616239	6	4	theme	centrifugation	1170:1183	arg1	cycles					1185:1190	multiple centrifugation cycles	1161:1190	multiple centrifugation cycles	1161:1190	The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials.					
35616239	8	5	theme	BSA/β-casein	1608:1619	arg1	mixture					1641:1647	a BSA/β-casein (mass ratio, 500/1) mixture	1606:1647	a BSA/β-casein (mass ratio, 500/1) mixture	1606:1647	As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture.					
35616239	1	6	theme	affinity	106:113	arg1	chromatography					115:128	Metal oxide affinity chromatography	94:128	Metal oxide affinity chromatography (MOAC)	94:135	Metal oxide affinity chromatography (MOAC) is considered to be one of the most effective methods for phosphopeptide enrichment.					
35616239	1	6	theme	affinity	106:113	arg1	MOAC					131:134	MOAC	131:134	MOAC	131:134	Metal oxide affinity chromatography (MOAC) is considered to be one of the most effective methods for phosphopeptide enrichment.					
35616239	3	7	theme	materials	539:547	arg1	reusability					518:528	the reusability	514:528	the reusability of these materials to achieve sustainability	514:573	Moreover, the reusability of these materials to achieve sustainability was hardly investigated.					
35616239	1	8	theme	effective	173:181	arg1	methods					183:189	the most effective methods	164:189	the most effective methods for phosphopeptide enrichment	164:219	Metal oxide affinity chromatography (MOAC) is considered to be one of the most effective methods for phosphopeptide enrichment.					
35616239	6	9	theme	tedious	1135:1141	arg1	implementation					1143:1156	the tedious implementation	1131:1156	the tedious implementation of multiple centrifugation cycles	1131:1190	The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials.					
35616239	10	10	theme	Hela	1958:1961	arg1	exosomes					1968:1975	Hela cell exosomes	1958:1975	Hela cell exosomes	1958:1975	Furthermore, 9287 unique phosphopeptides derived from the 2661 phosphoproteins were successfully identified from two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material.					
35616239	7	11	theme	CM	1376:1377	arg1	material					1379:1386	the CM material	1372:1386	the CM material	1372:1386	Moreover, a large amount of TiO2 was introduced onto the surface of the CM material, and thus, the resultant TiO2-CM material exhibited a large surface area.					
35616239	10	12	theme	digests	1947:1953	arg1	milligrams					1925:1934	two milligrams	1921:1934	two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material	1921:2065	Furthermore, 9287 unique phosphopeptides derived from the 2661 phosphoproteins were successfully identified from two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material.					
35616239	2	13	theme	frequent	284:291	arg1	centrifugation					293:306	frequent centrifugation	284:306	frequent centrifugation	284:306	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	6	14	theme	experimental	1232:1243	arg1	procedure					1245:1253	the experimental procedure	1228:1253	the experimental procedure	1228:1253	The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials.					
35616239	7	15	theme	large	1316:1320	arg1	TiO2					1332:1335	TiO2	1332:1335	TiO2	1332:1335	Moreover, a large amount of TiO2 was introduced onto the surface of the CM material, and thus, the resultant TiO2-CM material exhibited a large surface area.					
35616239	7	15	theme	large	1316:1320	arg1	amount					1322:1327	a large amount	1314:1327	a large amount of TiO2	1314:1335	Moreover, a large amount of TiO2 was introduced onto the surface of the CM material, and thus, the resultant TiO2-CM material exhibited a large surface area.					
35616239	4	16	theme	titanium	649:656	arg1	TiO2					667:670	TiO2	667:670	TiO2	667:670	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	4	16	theme	titanium	649:656	arg1	dioxide					658:664	inorganic titanium dioxide	639:664	inorganic titanium dioxide (TiO2)	639:671	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	5	17	theme	organic-inorganic	825:841	arg1	material					858:865	an organic-inorganic hybrid TiO2-CM material	822:865	an organic-inorganic hybrid TiO2-CM material	822:865	This produced an organic-inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition.					
35616239	10	18	theme	exosomes	1968:1975	arg1	digests					1947:1953	tryptic digests	1939:1953	tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material	1939:2065	Furthermore, 9287 unique phosphopeptides derived from the 2661 phosphoproteins were successfully identified from two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material.					
35616239	5	19	theme	organic	901:907	arg1	substances					923:932	organic and inorganic substances	901:932	organic and inorganic substances	901:932	This produced an organic-inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition.					
35616239	6	20	theme	materials	1293:1301	arg1	loss					1272:1275	the loss	1268:1275	the loss of peptides and materials	1268:1301	The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials.					
35616239	5	21	theme	inorganic	913:921	arg1	substances					923:932	organic and inorganic substances	901:932	organic and inorganic substances	901:932	This produced an organic-inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition.					
35616239	6	22	theme	prepared	998:1005	arg1	material					1015:1022	The prepared TiO2-CM material	994:1022	The prepared TiO2-CM material as monolith	994:1034	The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials.					
35616239	9	23	theme	commercial	1698:1707	arg1	beads					1714:1718	commercial TiO2 beads	1698:1718	commercial TiO2 beads	1698:1718	The results were superior to those achieved for commercial TiO2 beads, confirming that TiO2-CM has excellent selectivity for phosphopeptides and reusability.					
35616239	7	24	theme	TiO2	1332:1335	arg1	TiO2					1332:1335	TiO2	1332:1335	TiO2	1332:1335	Moreover, a large amount of TiO2 was introduced onto the surface of the CM material, and thus, the resultant TiO2-CM material exhibited a large surface area.					
35616239	7	24	theme	TiO2	1332:1335	arg1	amount					1322:1327	a large amount	1314:1327	a large amount of TiO2	1314:1335	Moreover, a large amount of TiO2 was introduced onto the surface of the CM material, and thus, the resultant TiO2-CM material exhibited a large surface area.					
35616239	10	25	theme	independent	1999:2009	arg1	replications					2011:2022	five independent replications	1994:2022	five independent replications after enriching using the TiO2-CM material	1994:2065	Furthermore, 9287 unique phosphopeptides derived from the 2661 phosphoproteins were successfully identified from two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material.					
35616239	0	26	theme	Phosphopeptides	77:91	arg1	Enrichment					63:72	Enrichment	63:72	Enrichment of Phosphopeptides	63:91	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides.					
35616239	8	27	dep	BSA/β-casein	1608:1619	arg1	ratio					1627:1631	mass ratio	1622:1631	mass ratio	1622:1631	As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture.					
35616239	10	28	theme	TiO2-CM	2050:2056	arg1	material					2058:2065	the TiO2-CM material	2046:2065	the TiO2-CM material	2046:2065	Furthermore, 9287 unique phosphopeptides derived from the 2661 phosphoproteins were successfully identified from two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material.					
35616239	12	29	theme	cheap	2226:2230	arg1	cellulose					2232:2240	cheap cellulose	2226:2240	cheap cellulose	2226:2240	Furthermore, TiO2-CM consists of green and cheap cellulose as the skeleton, and its synthesis process is environment-friendly, simple, and inexpensive.					
35616239	6	30	theme	multiple	1161:1168	arg1	cycles					1185:1190	multiple centrifugation cycles	1161:1190	multiple centrifugation cycles	1161:1190	The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials.					
35616239	2	31	theme	peptides	386:393	arg1	loss					378:381	loss	378:381	loss of peptides and materials	378:407	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	2	31	theme	peptides	386:393	arg1	clogging					413:420	clogging	413:420	clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS)	413:495	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	1	32	theme	oxide	100:104	arg1	chromatography					115:128	Metal oxide affinity chromatography	94:128	Metal oxide affinity chromatography (MOAC)	94:135	Metal oxide affinity chromatography (MOAC) is considered to be one of the most effective methods for phosphopeptide enrichment.					
35616239	1	32	theme	oxide	100:104	arg1	MOAC					131:134	MOAC	131:134	MOAC	131:134	Metal oxide affinity chromatography (MOAC) is considered to be one of the most effective methods for phosphopeptide enrichment.					
35616239	9	33	theme	excellent	1749:1757	arg1	selectivity					1759:1769	excellent selectivity	1749:1769	excellent selectivity for phosphopeptides and reusability	1749:1805	The results were superior to those achieved for commercial TiO2 beads, confirming that TiO2-CM has excellent selectivity for phosphopeptides and reusability.					
35616239	8	34	theme	TiO2-CM	1490:1496	arg1	result					1467:1472	a result	1465:1472	a result	1465:1472	As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture.					
35616239	8	34	theme	TiO2-CM	1490:1496	arg1	material					1498:1505	the fabricated TiO2-CM material	1475:1505	the fabricated TiO2-CM material	1475:1505	As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture.					
35616239	4	35	theme	cellulose	725:733	arg1	material					749:756	the organic cellulose monolith (CM) material	713:756	the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method	713:805	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	2	36	theme	loss	378:381	arg1	risks					369:373	potential risks	359:373	potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS)	359:495	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	0	37	theme	Robust	0:5	arg1	Monolith					50:57	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith	0:57	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides.	0:92	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides.					
35616239	6	38	theme	excellent	1046:1054	arg1	strength					1067:1074	excellent mechanical strength	1046:1074	excellent mechanical strength	1046:1074	The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials.					
35616239	11	39	theme	protein	2158:2164	arg1	phosphorylation					2166:2180	protein phosphorylation	2158:2180	protein phosphorylation	2158:2180	The results indicated that the material has good application prospects in the analysis of protein phosphorylation.					
35616239	2	40	theme	clogging	413:420	arg1	risks					369:373	potential risks	359:373	potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS)	359:495	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	0	41	theme	Titanium	23:30	arg1	Monolith					50:57	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith	0:57	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides.	0:92	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides.					
35616239	10	42	theme	unique	1826:1831	arg1	phosphopeptides					1833:1847	9287 unique phosphopeptides	1821:1847	9287 unique phosphopeptides derived from the 2661 phosphoproteins	1821:1885	Furthermore, 9287 unique phosphopeptides derived from the 2661 phosphoproteins were successfully identified from two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material.					
35616239	4	43	theme	sol-gel	792:798	arg1	method					800:805	a sol-gel method	790:805	a sol-gel method	790:805	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	2	44	theme	materials	399:407	arg1	loss					378:381	loss	378:381	loss of peptides and materials	378:407	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	2	44	theme	materials	399:407	arg1	clogging					413:420	clogging	413:420	clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS)	413:495	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	2	45	theme	liquid	449:454	arg1	LC-MS					490:494	LC-MS	490:494	LC-MS	490:494	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	2	45	theme	liquid	449:454	arg1	spectrometry					476:487	liquid chromatography-mass spectrometry	449:487	liquid chromatography-mass spectrometry (LC-MS)	449:495	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	4	46	theme	coral-like	765:774	arg1	structure					776:784	a coral-like structure	763:784	a coral-like structure via a sol-gel method	763:805	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	5	47	theme	TiO2-CM	850:856	arg1	material					858:865	an organic-inorganic hybrid TiO2-CM material	822:865	an organic-inorganic hybrid TiO2-CM material	822:865	This produced an organic-inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition.					
35616239	5	48	theme	composition	981:991	arg1	terms					972:976	terms	972:976	terms of composition	972:991	This produced an organic-inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition.					
35616239	8	49	theme	500/1	1634:1638	arg1	ratio					1627:1631	mass ratio	1622:1631	mass ratio	1622:1631	As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture.					
35616239	7	50	theme	large	1442:1446	arg1	area					1456:1459	a large surface area	1440:1459	a large surface area	1440:1459	Moreover, a large amount of TiO2 was introduced onto the surface of the CM material, and thus, the resultant TiO2-CM material exhibited a large surface area.					
35616239	8	51	theme	mixture	1641:1647	arg1	digests					1595:1601	the tryptic digests	1583:1601	the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture	1583:1647	As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture.					
35616239	11	52	theme	good	2112:2115	arg1	prospects					2129:2137	good application prospects	2112:2137	good application prospects	2112:2137	The results indicated that the material has good application prospects in the analysis of protein phosphorylation.					
35616239	7	53	theme	resultant	1403:1411	arg1	material					1421:1428	the resultant TiO2-CM material	1399:1428	the resultant TiO2-CM material	1399:1428	Moreover, a large amount of TiO2 was introduced onto the surface of the CM material, and thus, the resultant TiO2-CM material exhibited a large surface area.					
35616239	5	54	theme	hybrid	843:848	arg1	material					858:865	an organic-inorganic hybrid TiO2-CM material	822:865	an organic-inorganic hybrid TiO2-CM material	822:865	This produced an organic-inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition.					
35616239	7	55	theme	TiO2-CM	1413:1419	arg1	material					1421:1428	the resultant TiO2-CM material	1399:1428	the resultant TiO2-CM material	1399:1428	Moreover, a large amount of TiO2 was introduced onto the surface of the CM material, and thus, the resultant TiO2-CM material exhibited a large surface area.					
35616239	9	56	contain	has	1745:1747	arg1	TiO2-CM					1737:1743	TiO2-CM	1737:1743	TiO2-CM	1737:1743	The results were superior to those achieved for commercial TiO2 beads, confirming that TiO2-CM has excellent selectivity for phosphopeptides and reusability.					
35616239	9	56	contain	has	1745:1747	arg2	selectivity					1759:1769	excellent selectivity	1749:1769	excellent selectivity for phosphopeptides and reusability	1749:1805	The results were superior to those achieved for commercial TiO2 beads, confirming that TiO2-CM has excellent selectivity for phosphopeptides and reusability.					
35616239	1	57	theme	methods	183:189	arg1	methods					183:189	the most effective methods	164:189	the most effective methods for phosphopeptide enrichment	164:219	Metal oxide affinity chromatography (MOAC) is considered to be one of the most effective methods for phosphopeptide enrichment.					
35616239	1	57	theme	methods	183:189	arg1	one					157:159	one	157:159	one	157:159	Metal oxide affinity chromatography (MOAC) is considered to be one of the most effective methods for phosphopeptide enrichment.					
35616239	4	58	with	material	749:756	arg1	structure					776:784	a coral-like structure	763:784	a coral-like structure via a sol-gel method	763:805	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	1	59	theme	phosphopeptide	195:208	arg1	enrichment					210:219	phosphopeptide enrichment	195:219	phosphopeptide enrichment	195:219	Metal oxide affinity chromatography (MOAC) is considered to be one of the most effective methods for phosphopeptide enrichment.					
35616239	7	60	theme	material	1379:1386	arg1	surface					1361:1367	the surface	1357:1367	the surface of the CM material	1357:1386	Moreover, a large amount of TiO2 was introduced onto the surface of the CM material, and thus, the resultant TiO2-CM material exhibited a large surface area.					
35616239	11	61	contain	has	2108:2110	arg2	prospects					2129:2137	good application prospects	2112:2137	good application prospects	2112:2137	The results indicated that the material has good application prospects in the analysis of protein phosphorylation.					
35616239	11	61	contain	has	2108:2110	arg1	material					2099:2106	the material	2095:2106	the material	2095:2106	The results indicated that the material has good application prospects in the analysis of protein phosphorylation.					
35616239	10	62	theme	tryptic	1939:1945	arg1	digests					1947:1953	tryptic digests	1939:1953	tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material	1939:2065	Furthermore, 9287 unique phosphopeptides derived from the 2661 phosphoproteins were successfully identified from two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material.					
35616239	4	63	theme	inorganic	639:647	arg1	TiO2					667:670	TiO2	667:670	TiO2	667:670	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	4	63	theme	inorganic	639:647	arg1	dioxide					658:664	inorganic titanium dioxide	639:664	inorganic titanium dioxide (TiO2)	639:671	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	4	64	theme	skeletal	693:700	arg1	surface					702:708	the skeletal surface	689:708	the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method	689:805	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	10	65	theme	cell	1963:1966	arg1	exosomes					1968:1975	Hela cell exosomes	1958:1975	Hela cell exosomes	1958:1975	Furthermore, 9287 unique phosphopeptides derived from the 2661 phosphoproteins were successfully identified from two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material.					
35616239	6	66	theme	TiO2-CM	1007:1013	arg1	material					1015:1022	The prepared TiO2-CM material	994:1022	The prepared TiO2-CM material as monolith	994:1034	The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials.					
35616239	10	67	attach	derived	1849:1855	arg1	phosphoproteins					1871:1885	the 2661 phosphoproteins	1862:1885	the 2661 phosphoproteins	1862:1885	Furthermore, 9287 unique phosphopeptides derived from the 2661 phosphoproteins were successfully identified from two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material.					
35616239	10	67	attach	derived	1849:1855	arg2	phosphopeptides					1833:1847	9287 unique phosphopeptides	1821:1847	9287 unique phosphopeptides derived from the 2661 phosphoproteins	1821:1885	Furthermore, 9287 unique phosphopeptides derived from the 2661 phosphoproteins were successfully identified from two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material.					
35616239	9	68	theme	TiO2	1709:1712	arg1	beads					1714:1718	commercial TiO2 beads	1698:1718	commercial TiO2 beads	1698:1718	The results were superior to those achieved for commercial TiO2 beads, confirming that TiO2-CM has excellent selectivity for phosphopeptides and reusability.					
35616239	6	69	theme	cycles	1185:1190	arg1	implementation					1143:1156	the tedious implementation	1131:1156	the tedious implementation of multiple centrifugation cycles	1131:1190	The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials.					
35616239	5	70	contain	contained	874:882	arg2	combination					886:896	a combination	884:896	a combination of organic and inorganic substances	884:932	This produced an organic-inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition.					
35616239	5	70	contain	contained	874:882	arg1	material					858:865	an organic-inorganic hybrid TiO2-CM material	822:865	an organic-inorganic hybrid TiO2-CM material	822:865	This produced an organic-inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition.					
35616239	5	71	dep	it	942:943	arg1	mimic					945:949	mimic	945:949	mimic the mollusk shell in terms of composition	945:991	This produced an organic-inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition.					
35616239	8	72	theme	fabricated	1479:1488	arg1	result					1467:1472	a result	1465:1472	a result	1465:1472	As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture.					
35616239	8	72	theme	fabricated	1479:1488	arg1	material					1498:1505	the fabricated TiO2-CM material	1475:1505	the fabricated TiO2-CM material	1475:1505	As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture.					
35616239	4	73	theme	CM	745:746	arg1	material					749:756	the organic cellulose monolith (CM) material	713:756	the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method	713:805	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	1	74	theme	Metal	94:98	arg1	chromatography					115:128	Metal oxide affinity chromatography	94:128	Metal oxide affinity chromatography (MOAC)	94:135	Metal oxide affinity chromatography (MOAC) is considered to be one of the most effective methods for phosphopeptide enrichment.					
35616239	1	74	theme	Metal	94:98	arg1	MOAC					131:134	MOAC	131:134	MOAC	131:134	Metal oxide affinity chromatography (MOAC) is considered to be one of the most effective methods for phosphopeptide enrichment.					
35616239	4	75	theme	material	749:756	arg1	surface					702:708	the skeletal surface	689:708	the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method	689:805	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	0	76	theme	Dual-Biomimetic	7:21	arg1	Monolith					50:57	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith	0:57	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides.	0:92	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides.					
35616239	5	77	theme	substances	923:932	arg1	combination					886:896	a combination	884:896	a combination of organic and inorganic substances	884:932	This produced an organic-inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition.					
35616239	6	78	theme	mechanical	1056:1065	arg1	strength					1067:1074	excellent mechanical strength	1046:1074	excellent mechanical strength	1046:1074	The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials.					
35616239	2	79	theme	column	429:434	arg1	loss					378:381	loss	378:381	loss of peptides and materials	378:407	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	2	79	theme	column	429:434	arg1	clogging					413:420	clogging	413:420	clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS)	413:495	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	4	80	theme	organic	717:723	arg1	material					749:756	the organic cellulose monolith (CM) material	713:756	the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method	713:805	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	0	81	theme	Dioxide-Cellulose	32:48	arg1	Monolith					50:57	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith	0:57	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides.	0:92	Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides.					
35616239	11	82	theme	phosphorylation	2166:2180	arg1	analysis					2146:2153	the analysis	2142:2153	the analysis of protein phosphorylation	2142:2180	The results indicated that the material has good application prospects in the analysis of protein phosphorylation.					
35616239	5	83	theme	mollusk	955:961	arg1	shell					963:967	the mollusk shell	951:967	the mollusk shell	951:967	This produced an organic-inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition.					
35616239	4	84	theme	monolith	735:742	arg1	material					749:756	the organic cellulose monolith (CM) material	713:756	the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method	713:805	To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol-gel method.					
35616239	2	85	theme	chromatography-mass	456:474	arg1	LC-MS					490:494	LC-MS	490:494	LC-MS	490:494	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	2	85	theme	chromatography-mass	456:474	arg1	spectrometry					476:487	liquid chromatography-mass spectrometry	449:487	liquid chromatography-mass spectrometry (LC-MS)	449:495	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	8	86	theme	phosphopeptides	1553:1567	arg1	enrichment					1539:1548	the enrichment	1535:1548	the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture	1535:1647	As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture.					
35616239	2	87	theme	enrichment	335:344	arg1	process					346:352	the enrichment process	331:352	the enrichment process	331:352	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
35616239	8	88	theme	mass	1622:1625	arg1	ratio					1627:1631	mass ratio	1622:1631	mass ratio	1622:1631	As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture.					
35616239	6	89	theme	enrichment	1105:1114	arg1	process					1116:1122	the enrichment process	1101:1122	the enrichment process	1101:1122	The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials.					
35616239	8	90	theme	tryptic	1587:1593	arg1	digests					1595:1601	the tryptic digests	1583:1601	the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture	1583:1647	As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture.					
35616239	5	91	dep	produced	813:820	arg1	making					935:940	making	935:940	making it mimic the mollusk shell in terms of composition	935:991	This produced an organic-inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition.					
35616239	2	92	theme	potential	359:367	arg1	risks					369:373	potential risks	359:373	potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS)	359:495	However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography-mass spectrometry (LC-MS) arise.					
36271212	0	0	theme	In	84:85	arg1	Evaluation					102:111	In Vitro, Ex Vivo Evaluation	84:111	In Vitro, Ex Vivo Evaluation	84:111	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.					
36271212	5	1	theme	in	654:655	arg1	gel					662:664	The developed in situ gel	640:664	The developed in situ gel	640:664	The developed in situ gel showed gelation temperatures (28-33°C), pH (4.5-6.5), rheological properties (pseudoplastic, shear thinning), and mucoadhesive strength (1755.78-2495.05 dyne/cm2).					
36271212	7	2	theme	release	965:971	arg1	kinetics					973:980	The release kinetics	961:980	The release kinetics	961:980	The release kinetics fit to the Korsmeyer-Peppas model, suggesting the non-Fickian diffusion type controlled release behavior.					
36271212	5	3	theme	shear	759:763	arg1	pseudoplastic					744:756	pseudoplastic	744:756	pseudoplastic	744:756	The developed in situ gel showed gelation temperatures (28-33°C), pH (4.5-6.5), rheological properties (pseudoplastic, shear thinning), and mucoadhesive strength (1755.78-2495.05 dyne/cm2).					
36271212	5	3	theme	shear	759:763	arg1	thinning					765:772	shear thinning	759:772	shear thinning	759:772	The developed in situ gel showed gelation temperatures (28-33°C), pH (4.5-6.5), rheological properties (pseudoplastic, shear thinning), and mucoadhesive strength (1755.78-2495.05 dyne/cm2).					
36271212	4	4	theme	thermoreversible	520:535	arg1	composition					541:551	the thermoreversible gel composition	516:551	the thermoreversible gel composition	516:551	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	6	5	contain	containing	910:919	arg2	retardation					933:943	significant retardation	921:943	significant retardation of CBZ release	921:958	The in vitro release study has shown sustained release behavior (24 h) for gel, containing significant retardation of CBZ release.					
36271212	6	5	contain	containing	910:919	arg1	gel					905:907	gel	905:907	gel	905:907	The in vitro release study has shown sustained release behavior (24 h) for gel, containing significant retardation of CBZ release.					
36271212	8	6	from	gel	1167:1169	arg1	release					1150:1156	sustained release	1140:1156	sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2)	1140:1248	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	8	6	from	gel	1167:1169	arg1	flux					1267:1270	a permeation flux	1254:1270	a permeation flux of 10.16 µg/cm2/h	1254:1288	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	1	7	theme	intranasal	136:145	arg1	delivery					152:159	intranasal (IN) delivery	136:159	intranasal (IN) delivery	136:159	Over the past decade, intranasal (IN) delivery has been gaining attention as an alternative approach to conventional drug delivery routes targeting the brain.					
36271212	1	7	theme	intranasal	136:145	arg1	IN					148:149	IN	148:149	IN	148:149	Over the past decade, intranasal (IN) delivery has been gaining attention as an alternative approach to conventional drug delivery routes targeting the brain.					
36271212	10	8	theme	nasal	1411:1415	arg1	mucosa					1417:1422	goat nasal mucosa	1406:1422	goat nasal mucosa using in situ gel	1406:1440	Permeation through goat nasal mucosa using in situ gel has demonstrated a harmless nasal delivery, which can provide a new dimension to deliver CBZ directly to the brain bypassing the blood-brain barrier.					
36271212	1	9	theme	conventional	218:229	arg1	routes					245:250	conventional drug delivery routes	218:250	conventional drug delivery routes targeting the brain	218:270	Over the past decade, intranasal (IN) delivery has been gaining attention as an alternative approach to conventional drug delivery routes targeting the brain.					
36271212	7	10	theme	type	1054:1057	arg1	behavior					1078:1085	the non-Fickian diffusion type controlled release behavior	1028:1085	the non-Fickian diffusion type controlled release behavior	1028:1085	The release kinetics fit to the Korsmeyer-Peppas model, suggesting the non-Fickian diffusion type controlled release behavior.					
36271212	9	11	theme	mucosa	1359:1364	arg1	function					1341:1348	the barrier function	1329:1348	the barrier function of nasal mucosa	1329:1364	After treatment with CBZ in situ gel, the barrier function of nasal mucosa remained unaffected.					
36271212	7	12	theme	release	1070:1076	arg1	behavior					1078:1085	the non-Fickian diffusion type controlled release behavior	1028:1085	the non-Fickian diffusion type controlled release behavior	1028:1085	The release kinetics fit to the Korsmeyer-Peppas model, suggesting the non-Fickian diffusion type controlled release behavior.					
36271212	1	13	theme	drug	231:234	arg1	routes					245:250	conventional drug delivery routes	218:250	conventional drug delivery routes targeting the brain	218:270	Over the past decade, intranasal (IN) delivery has been gaining attention as an alternative approach to conventional drug delivery routes targeting the brain.					
36271212	7	14	theme	controlled	1059:1068	arg1	behavior					1078:1085	the non-Fickian diffusion type controlled release behavior	1028:1085	the non-Fickian diffusion type controlled release behavior	1028:1085	The release kinetics fit to the Korsmeyer-Peppas model, suggesting the non-Fickian diffusion type controlled release behavior.					
36271212	10	15	theme	blood-brain	1571:1581	arg1	barrier					1583:1589	the blood-brain barrier	1567:1589	the blood-brain barrier	1567:1589	Permeation through goat nasal mucosa using in situ gel has demonstrated a harmless nasal delivery, which can provide a new dimension to deliver CBZ directly to the brain bypassing the blood-brain barrier.					
36271212	10	16	theme	harmless	1461:1468	arg1	delivery					1476:1483	a harmless nasal delivery	1459:1483	a harmless nasal delivery	1459:1483	Permeation through goat nasal mucosa using in situ gel has demonstrated a harmless nasal delivery, which can provide a new dimension to deliver CBZ directly to the brain bypassing the blood-brain barrier.					
36271212	0	17	dep	Ex	94:95	arg1	Vivo					97:100	Vivo	97:100	Vivo	97:100	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.					
36271212	1	18	theme	delivery	236:243	arg1	routes					245:250	conventional drug delivery routes	218:250	conventional drug delivery routes targeting the brain	218:270	Over the past decade, intranasal (IN) delivery has been gaining attention as an alternative approach to conventional drug delivery routes targeting the brain.					
36271212	7	19	theme	non-Fickian	1032:1042	arg1	behavior					1078:1085	the non-Fickian diffusion type controlled release behavior	1028:1085	the non-Fickian diffusion type controlled release behavior	1028:1085	The release kinetics fit to the Korsmeyer-Peppas model, suggesting the non-Fickian diffusion type controlled release behavior.					
36271212	8	20	theme	nasal	1120:1124	arg1	mucosa					1126:1131	goat nasal mucosa	1115:1131	goat nasal mucosa	1115:1131	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	8	21	theme	cumulative	1208:1217	arg1	drug					1219:1222	the highest cumulative drug	1196:1222	the highest cumulative drug permeated (243.94 µg/cm2)	1196:1248	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	8	21	theme	cumulative	1208:1217	arg1	243.94 µg/cm2					1235:1247	243.94 µg/cm2	1235:1247	243.94 µg/cm2	1235:1247	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	8	22	theme	goat	1115:1118	arg1	mucosa					1126:1131	goat nasal mucosa	1115:1131	goat nasal mucosa	1115:1131	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	4	23	theme	%	587:587	arg1	poloxamer					560:568	poloxamer 407	560:572	poloxamer 407 (P407) (15-20% w/v)	560:592	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	4	23	theme	%	587:587	arg1	w/v					589:591	15-20% w/v	582:591	15-20% w/v	582:591	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	4	24	theme	gel	537:539	arg1	composition					541:551	the thermoreversible gel composition	516:551	the thermoreversible gel composition	516:551	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	8	25	theme	18	1182:1183	arg1	%					1184:1184	%	1184:1184	%	1184:1184	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	4	26	used	used	488:491	arg2	method					459:464	A cold method	452:464	A cold method of synthesis	452:477	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	8	27	theme	highest	1200:1206	arg1	drug					1219:1222	the highest cumulative drug	1196:1222	the highest cumulative drug permeated (243.94 µg/cm2)	1196:1248	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	8	27	theme	highest	1200:1206	arg1	243.94 µg/cm2					1235:1247	243.94 µg/cm2	1235:1247	243.94 µg/cm2	1235:1247	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	3	28	theme	gelling	404:410	arg1	system					412:417	a thermoreversible in situ gelling system	377:417	a thermoreversible in situ gelling system for delivering CBZ via IN route	377:449	The present study aims to develop a thermoreversible in situ gelling system for delivering CBZ via IN route.					
36271212	0	29	theme	Carbamazepine	17:29	arg1	Gel					39:41	Thermoreversible Carbamazepine In Situ Gel	0:41	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.	0:112	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.					
36271212	0	30	dep	In	84:85	arg1	Ex					94:95	Ex	94:95	Ex	94:95	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.					
36271212	0	30	dep	In	84:85	arg1	Vitro					87:91	Vitro	87:91	Vitro	87:91	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.					
36271212	3	31	theme	thermoreversible	379:394	arg1	system					412:417	a thermoreversible in situ gelling system	377:417	a thermoreversible in situ gelling system for delivering CBZ via IN route	377:449	The present study aims to develop a thermoreversible in situ gelling system for delivering CBZ via IN route.					
36271212	0	32	theme	Thermoreversible	0:15	arg1	Gel					39:41	Thermoreversible Carbamazepine In Situ Gel	0:41	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.	0:112	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.					
36271212	6	33	theme	in	834:835	arg1	study					851:855	The in vitro release study	830:855	The in vitro release study	830:855	The in vitro release study has shown sustained release behavior (24 h) for gel, containing significant retardation of CBZ release.					
36271212	4	34	theme	%	632:632	arg1	carrageenan					603:613	iota carrageenan	598:613	iota carrageenan (ɩ-Cg) (0.15-0.25% w/v)	598:637	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	4	34	theme	%	632:632	arg1	w/v					634:636	0.15-0.25% w/v	623:636	0.15-0.25% w/v	623:636	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	3	35	theme	in	396:397	arg1	system					412:417	a thermoreversible in situ gelling system	377:417	a thermoreversible in situ gelling system for delivering CBZ via IN route	377:449	The present study aims to develop a thermoreversible in situ gelling system for delivering CBZ via IN route.					
36271212	2	36	theme	ingestible	319:328	arg1	formulation					330:340	an orally ingestible formulation	309:340	an orally ingestible formulation	309:340	Carbamazepine (CBZ) is available as an orally ingestible formulation.					
36271212	5	37	dep	in	654:655	arg1	situ					657:660	situ	657:660	situ	657:660	The developed in situ gel showed gelation temperatures (28-33°C), pH (4.5-6.5), rheological properties (pseudoplastic, shear thinning), and mucoadhesive strength (1755.78-2495.05 dyne/cm2).					
36271212	5	38	theme	mucoadhesive	780:791	arg1	strength					793:800	mucoadhesive strength	780:800	mucoadhesive strength (1755.78-2495.05 dyne/cm2)	780:827	The developed in situ gel showed gelation temperatures (28-33°C), pH (4.5-6.5), rheological properties (pseudoplastic, shear thinning), and mucoadhesive strength (1755.78-2495.05 dyne/cm2).					
36271212	5	38	theme	mucoadhesive	780:791	arg1	dyne/cm2					819:826	1755.78-2495.05 dyne/cm2	803:826	1755.78-2495.05 dyne/cm2	803:826	The developed in situ gel showed gelation temperatures (28-33°C), pH (4.5-6.5), rheological properties (pseudoplastic, shear thinning), and mucoadhesive strength (1755.78-2495.05 dyne/cm2).					
36271212	9	39	theme	in	1316:1317	arg1	gel					1324:1326	CBZ in situ gel	1312:1326	CBZ in situ gel	1312:1326	After treatment with CBZ in situ gel, the barrier function of nasal mucosa remained unaffected.					
36271212	0	40	theme	In	31:32	arg1	Gel					39:41	Thermoreversible Carbamazepine In Situ Gel	0:41	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.	0:112	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.					
36271212	6	41	theme	sustained	867:875	arg1	24 h					895:898	24 h	895:898	24 h	895:898	The in vitro release study has shown sustained release behavior (24 h) for gel, containing significant retardation of CBZ release.					
36271212	6	41	theme	sustained	867:875	arg1	behavior					885:892	sustained release behavior	867:892	sustained release behavior (24 h)	867:899	The in vitro release study has shown sustained release behavior (24 h) for gel, containing significant retardation of CBZ release.					
36271212	8	42	theme	%	1184:1184	arg1	P407					1186:1189	18% P407	1182:1189	18% P407 with the highest cumulative drug permeated (243.94 µg/cm2)	1182:1248	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	4	43	theme	iota	598:601	arg1	carrageenan					603:613	iota carrageenan	598:613	iota carrageenan (ɩ-Cg) (0.15-0.25% w/v)	598:637	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	4	43	theme	iota	598:601	arg1	w/v					634:636	0.15-0.25% w/v	623:636	0.15-0.25% w/v	623:636	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	4	43	theme	iota	598:601	arg1	ɩ-Cg					616:619	ɩ-Cg	616:619	ɩ-Cg	616:619	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	8	44	contain	containing	1171:1180	arg2	P407					1186:1189	18% P407	1182:1189	18% P407 with the highest cumulative drug permeated (243.94 µg/cm2)	1182:1248	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	8	44	contain	containing	1171:1180	arg1	gel					1167:1169	the gel	1163:1169	the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2)	1163:1248	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	10	45	theme	new	1506:1508	arg1	dimension					1510:1518	a new dimension	1504:1518	a new dimension to deliver CBZ directly to the brain bypassing the blood-brain barrier	1504:1589	Permeation through goat nasal mucosa using in situ gel has demonstrated a harmless nasal delivery, which can provide a new dimension to deliver CBZ directly to the brain bypassing the blood-brain barrier.					
36271212	5	46	dep	properties	732:741	arg1	pseudoplastic					744:756	pseudoplastic	744:756	pseudoplastic	744:756	The developed in situ gel showed gelation temperatures (28-33°C), pH (4.5-6.5), rheological properties (pseudoplastic, shear thinning), and mucoadhesive strength (1755.78-2495.05 dyne/cm2).					
36271212	5	46	dep	properties	732:741	arg1	thinning					765:772	shear thinning	759:772	shear thinning	759:772	The developed in situ gel showed gelation temperatures (28-33°C), pH (4.5-6.5), rheological properties (pseudoplastic, shear thinning), and mucoadhesive strength (1755.78-2495.05 dyne/cm2).					
36271212	9	47	theme	CBZ	1312:1314	arg1	gel					1324:1326	CBZ in situ gel	1312:1326	CBZ in situ gel	1312:1326	After treatment with CBZ in situ gel, the barrier function of nasal mucosa remained unaffected.					
36271212	8	48	theme	sustained	1140:1148	arg1	release					1150:1156	sustained release	1140:1156	sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2)	1140:1248	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	6	49	theme	significant	921:931	arg1	retardation					933:943	significant retardation	921:943	significant retardation of CBZ release	921:958	The in vitro release study has shown sustained release behavior (24 h) for gel, containing significant retardation of CBZ release.					
36271212	7	50	theme	Korsmeyer-Peppas	993:1008	arg1	model					1010:1014	the Korsmeyer-Peppas model	989:1014	the Korsmeyer-Peppas model	989:1014	The release kinetics fit to the Korsmeyer-Peppas model, suggesting the non-Fickian diffusion type controlled release behavior.					
36271212	3	51	dep	in	396:397	arg1	situ					399:402	situ	399:402	situ	399:402	The present study aims to develop a thermoreversible in situ gelling system for delivering CBZ via IN route.					
36271212	7	52	theme	diffusion	1044:1052	arg1	behavior					1078:1085	the non-Fickian diffusion type controlled release behavior	1028:1085	the non-Fickian diffusion type controlled release behavior	1028:1085	The release kinetics fit to the Korsmeyer-Peppas model, suggesting the non-Fickian diffusion type controlled release behavior.					
36271212	5	53	theme	gelation	673:680	arg1	28-33°C					696:702	28-33°C	696:702	28-33°C	696:702	The developed in situ gel showed gelation temperatures (28-33°C), pH (4.5-6.5), rheological properties (pseudoplastic, shear thinning), and mucoadhesive strength (1755.78-2495.05 dyne/cm2).					
36271212	5	53	theme	gelation	673:680	arg1	temperatures					682:693	gelation temperatures	673:693	gelation temperatures (28-33°C)	673:703	The developed in situ gel showed gelation temperatures (28-33°C), pH (4.5-6.5), rheological properties (pseudoplastic, shear thinning), and mucoadhesive strength (1755.78-2495.05 dyne/cm2).					
36271212	4	54	theme	synthesis	469:477	arg1	method					459:464	A cold method	452:464	A cold method of synthesis	452:477	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	10	55	theme	in	1430:1431	arg1	gel					1438:1440	in situ gel	1430:1440	in situ gel	1430:1440	Permeation through goat nasal mucosa using in situ gel has demonstrated a harmless nasal delivery, which can provide a new dimension to deliver CBZ directly to the brain bypassing the blood-brain barrier.					
36271212	8	56	theme	permeation	1256:1265	arg1	flux					1267:1270	a permeation flux	1254:1270	a permeation flux of 10.16 µg/cm2/h	1254:1288	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	6	57	theme	release	952:958	arg1	retardation					933:943	significant retardation	921:943	significant retardation of CBZ release	921:958	The in vitro release study has shown sustained release behavior (24 h) for gel, containing significant retardation of CBZ release.					
36271212	10	58	dep	in	1430:1431	arg1	situ					1433:1436	situ	1433:1436	situ	1433:1436	Permeation through goat nasal mucosa using in situ gel has demonstrated a harmless nasal delivery, which can provide a new dimension to deliver CBZ directly to the brain bypassing the blood-brain barrier.					
36271212	0	59	theme	Intranasal	47:56	arg1	Delivery					58:65	Intranasal Delivery	47:65	Intranasal Delivery	47:65	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.					
36271212	8	60	dep	Ex	1088:1089	arg1	vivo					1091:1094	vivo	1091:1094	vivo	1091:1094	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	8	61	with	P407	1186:1189	arg1	drug					1219:1222	the highest cumulative drug	1196:1222	the highest cumulative drug permeated (243.94 µg/cm2)	1196:1248	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	8	61	with	P407	1186:1189	arg1	243.94 µg/cm2					1235:1247	243.94 µg/cm2	1235:1247	243.94 µg/cm2	1235:1247	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	0	62	dep	Gel	39:41	arg1	Evaluation					102:111	In Vitro, Ex Vivo Evaluation	84:111	In Vitro, Ex Vivo Evaluation	84:111	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.					
36271212	0	62	dep	Gel	39:41	arg1	Development					68:78	Development	68:78	Development	68:78	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.					
36271212	9	63	theme	barrier	1333:1339	arg1	function					1341:1348	the barrier function	1329:1348	the barrier function of nasal mucosa	1329:1364	After treatment with CBZ in situ gel, the barrier function of nasal mucosa remained unaffected.					
36271212	4	64	theme	0.15-0.25	623:631	arg1	carrageenan					603:613	iota carrageenan	598:613	iota carrageenan (ɩ-Cg) (0.15-0.25% w/v)	598:637	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	4	64	theme	0.15-0.25	623:631	arg1	w/v					634:636	0.15-0.25% w/v	623:636	0.15-0.25% w/v	623:636	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	6	65	theme	release	843:849	arg1	study					851:855	The in vitro release study	830:855	The in vitro release study	830:855	The in vitro release study has shown sustained release behavior (24 h) for gel, containing significant retardation of CBZ release.					
36271212	9	66	with	treatment	1297:1305	arg1	gel					1324:1326	CBZ in situ gel	1312:1326	CBZ in situ gel	1312:1326	After treatment with CBZ in situ gel, the barrier function of nasal mucosa remained unaffected.					
36271212	6	67	dep	in	834:835	arg1	vitro					837:841	vitro	837:841	vitro	837:841	The in vitro release study has shown sustained release behavior (24 h) for gel, containing significant retardation of CBZ release.					
36271212	0	68	dep	In	31:32	arg1	Situ					34:37	Situ	34:37	Situ	34:37	Thermoreversible Carbamazepine In Situ Gel for Intranasal Delivery: Development and In Vitro, Ex Vivo Evaluation.					
36271212	3	69	theme	IN	442:443	arg1	route					445:449	IN route	442:449	IN route	442:449	The present study aims to develop a thermoreversible in situ gelling system for delivering CBZ via IN route.					
36271212	8	70	theme	Ex	1088:1089	arg1	permeation					1096:1105	Ex vivo permeation	1088:1105	Ex vivo permeation through goat nasal mucosa	1088:1131	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	6	71	theme	release	877:883	arg1	24 h					895:898	24 h	895:898	24 h	895:898	The in vitro release study has shown sustained release behavior (24 h) for gel, containing significant retardation of CBZ release.					
36271212	6	71	theme	release	877:883	arg1	behavior					885:892	sustained release behavior	867:892	sustained release behavior (24 h)	867:899	The in vitro release study has shown sustained release behavior (24 h) for gel, containing significant retardation of CBZ release.					
36271212	10	72	theme	goat	1406:1409	arg1	mucosa					1417:1422	goat nasal mucosa	1406:1422	goat nasal mucosa using in situ gel	1406:1440	Permeation through goat nasal mucosa using in situ gel has demonstrated a harmless nasal delivery, which can provide a new dimension to deliver CBZ directly to the brain bypassing the blood-brain barrier.					
36271212	6	73	theme	CBZ	948:950	arg1	release					952:958	CBZ release	948:958	CBZ release	948:958	The in vitro release study has shown sustained release behavior (24 h) for gel, containing significant retardation of CBZ release.					
36271212	9	74	dep	in	1316:1317	arg1	situ					1319:1322	situ	1319:1322	situ	1319:1322	After treatment with CBZ in situ gel, the barrier function of nasal mucosa remained unaffected.					
36271212	9	75	theme	nasal	1353:1357	arg1	mucosa					1359:1364	nasal mucosa	1353:1364	nasal mucosa	1353:1364	After treatment with CBZ in situ gel, the barrier function of nasal mucosa remained unaffected.					
36271212	1	76	theme	past	123:126	arg1	decade					128:133	the past decade	119:133	the past decade	119:133	Over the past decade, intranasal (IN) delivery has been gaining attention as an alternative approach to conventional drug delivery routes targeting the brain.					
36271212	5	77	theme	rheological	720:730	arg1	properties					732:741	rheological properties	720:741	rheological properties (pseudoplastic, shear thinning)	720:773	The developed in situ gel showed gelation temperatures (28-33°C), pH (4.5-6.5), rheological properties (pseudoplastic, shear thinning), and mucoadhesive strength (1755.78-2495.05 dyne/cm2).					
36271212	1	78	theme	alternative	194:204	arg1	approach					206:213	an alternative approach	191:213	an alternative approach to conventional drug delivery routes targeting the brain	191:270	Over the past decade, intranasal (IN) delivery has been gaining attention as an alternative approach to conventional drug delivery routes targeting the brain.					
36271212	1	78	theme	alternative	194:204	arg1	attention					178:186	attention	178:186	attention	178:186	Over the past decade, intranasal (IN) delivery has been gaining attention as an alternative approach to conventional drug delivery routes targeting the brain.					
36271212	5	79	theme	developed	644:652	arg1	gel					662:664	The developed in situ gel	640:664	The developed in situ gel	640:664	The developed in situ gel showed gelation temperatures (28-33°C), pH (4.5-6.5), rheological properties (pseudoplastic, shear thinning), and mucoadhesive strength (1755.78-2495.05 dyne/cm2).					
36271212	4	80	theme	cold	454:457	arg1	method					459:464	A cold method	452:464	A cold method of synthesis	452:477	A cold method of synthesis has been used to tailor and optimize the thermoreversible gel composition, using poloxamer 407 (P407) (15-20% w/v) and iota carrageenan (ɩ-Cg) (0.15-0.25% w/v).					
36271212	8	81	theme	10.16 µg/cm2/h	1275:1288	arg1	release					1150:1156	sustained release	1140:1156	sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2)	1140:1248	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	8	81	theme	10.16 µg/cm2/h	1275:1288	arg1	flux					1267:1270	a permeation flux	1254:1270	a permeation flux of 10.16 µg/cm2/h	1254:1288	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	3	82	theme	present	347:353	arg1	study					355:359	The present study	343:359	The present study	343:359	The present study aims to develop a thermoreversible in situ gelling system for delivering CBZ via IN route.					
36271212	10	83	theme	nasal	1470:1474	arg1	delivery					1476:1483	a harmless nasal delivery	1459:1483	a harmless nasal delivery	1459:1483	Permeation through goat nasal mucosa using in situ gel has demonstrated a harmless nasal delivery, which can provide a new dimension to deliver CBZ directly to the brain bypassing the blood-brain barrier.					
36271212	8	84	dep	permeated	1224:1232	arg1	drug					1219:1222	the highest cumulative drug	1196:1222	the highest cumulative drug permeated (243.94 µg/cm2)	1196:1248	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
36271212	8	84	dep	permeated	1224:1232	arg1	243.94 µg/cm2					1235:1247	243.94 µg/cm2	1235:1247	243.94 µg/cm2	1235:1247	Ex vivo permeation through goat nasal mucosa showed sustained release from the gel containing 18% P407 with the highest cumulative drug permeated (243.94 µg/cm2) and a permeation flux of 10.16 µg/cm2/h.					
35680754	10	0	theme	surface	1560:1566	arg1	mechanisms					1581:1590	surface complexation mechanisms	1560:1590	surface complexation mechanisms	1560:1590	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	0	1	from	study	14:18	arg1	Fe					44:45	Fe	44:45	Fe	44:45	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	0	1	from	study	14:18	arg1	III					26:28	III	26:28	III	26:28	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	0	1	from	study	14:18	arg1	III					47:49	III	47:49	III	47:49	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	0	1	from	study	14:18	arg1	Fe					23:24	Fe	23:24	Fe	23:24	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	5	2	dep	model	900:904	arg1	0.987					922:926	0.987	922:926	0.987	922:926	The adsorption kinetics was best described by the pseudo-second-order model (R2 = 0.998 and 0.992), whereas the adsorption isotherm was fitted well by the Freundlich model (R2 = 0.963 and 0.987).					
35680754	5	2	dep	model	900:904	arg1	R2 = 0.963					907:916	R2 = 0.963	907:916	R2 = 0.963	907:916	The adsorption kinetics was best described by the pseudo-second-order model (R2 = 0.998 and 0.992), whereas the adsorption isotherm was fitted well by the Freundlich model (R2 = 0.963 and 0.987).					
35680754	6	3	theme	Fe	1118:1119	arg1	-chitosan-CTAB					1125:1138	Fe(III)-chitosan-CTAB	1118:1138	Fe(III)-chitosan-CTAB	1118:1138	The presence of H2PO4- significantly inhibited the adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB, and humic acid also led to a slight decrease in As(V) adsorption by Fe(III)-chitosan-CTAB.					
35680754	1	4	theme	-chitosan	168:176	arg1	composites					204:213	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites	161:213	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites	161:213	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites were prepared using an ionotropic gelation method.					
35680754	1	5	theme	gelation	249:256	arg1	method					258:263	an ionotropic gelation method	235:263	an ionotropic gelation method	235:263	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites were prepared using an ionotropic gelation method.					
35680754	7	6	theme	Over	1141:1144	arg1	%					1148:1148	94%	1146:1148	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1	1141:1211	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	7	6	theme	Over	1141:1144	arg1	V					1156:1156	V	1156:1156	V	1156:1156	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	7	6	theme	Over	1141:1144	arg1	As					1153:1154	As	1153:1154	As	1153:1154	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	2	7	theme	Various	266:272	arg1	techniques					274:283	Various techniques	266:283	Various techniques	266:283	Various techniques were used to analyze the morphology, structure, and property of the adsorbents, including SEM, EDS, FT-IR, XPS, and zeta potential.					
35680754	2	8	theme	zeta	401:404	arg1	potential					406:414	zeta potential	401:414	zeta potential	401:414	Various techniques were used to analyze the morphology, structure, and property of the adsorbents, including SEM, EDS, FT-IR, XPS, and zeta potential.					
35680754	4	9	theme	As	562:563	arg1	adsorption					548:557	The adsorption	544:557	The adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB	544:614	The adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB could reach equilibrium in 20 min, and their maximum adsorption capacities were 33.85 and 31.69 mg g‒1, respectively.					
35680754	1	10	theme	Fe	182:183	arg1	composites					204:213	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites	161:213	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites	161:213	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites were prepared using an ionotropic gelation method.					
35680754	9	11	theme	Fe	1344:1345	arg1	-chitosan					1351:1359	Fe(III)-chitosan	1344:1359	Fe(III)-chitosan	1344:1359	Fe(III)-chitosan and Fe(III)-chitosan-CTAB still maintained their initial adsorption capacities after five adsorption-desorption cycles.					
35680754	4	12	dep	-chitosan-CTAB	601:614	arg1	III					597:599	III	597:599	III	597:599	The adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB could reach equilibrium in 20 min, and their maximum adsorption capacities were 33.85 and 31.69 mg g‒1, respectively.					
35680754	9	13	dep	-chitosan	1351:1359	arg1	-chitosan-CTAB					1372:1385	-chitosan-CTAB	1372:1385	-chitosan-CTAB	1372:1385	Fe(III)-chitosan and Fe(III)-chitosan-CTAB still maintained their initial adsorption capacities after five adsorption-desorption cycles.					
35680754	9	13	dep	-chitosan	1351:1359	arg1	III					1347:1349	III	1347:1349	III	1347:1349	Fe(III)-chitosan and Fe(III)-chitosan-CTAB still maintained their initial adsorption capacities after five adsorption-desorption cycles.					
35680754	10	14	from	adsorption	1624:1633	arg1	Fe					1638:1639	Fe	1638:1639	Fe	1638:1639	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	10	14	from	adsorption	1624:1633	arg1	III					1662:1664	III	1662:1664	III	1662:1664	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	10	14	from	adsorption	1624:1633	arg1	Fe					1659:1660	Fe	1659:1660	Fe	1659:1660	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	10	14	from	adsorption	1624:1633	arg1	III					1641:1643	III	1641:1643	III	1641:1643	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	8	15	theme	NaOH	1273:1276	arg1	solution					1278:1285	1% (w/v) NaOH solution	1264:1285	1% (w/v) NaOH solution	1264:1285	1% (w/v) NaOH solution was determined to be the most suitable desorption agent.					
35680754	8	15	theme	NaOH	1273:1276	arg1	agent					1337:1341	the most suitable desorption agent	1308:1341	the most suitable desorption agent	1308:1341	1% (w/v) NaOH solution was determined to be the most suitable desorption agent.					
35680754	3	16	dep	adsorption	500:509	arg1	V					497:497	V	497:497	V	497:497	Compared with Fe(III)-chitosan, Fe(III)-chitosan-CTAB was more effective for As(V) adsorption at a wide range of pH (3.0-8.0).					
35680754	5	17	theme	adsorption	846:855	arg1	isotherm					857:864	the adsorption isotherm	842:864	the adsorption isotherm	842:864	The adsorption kinetics was best described by the pseudo-second-order model (R2 = 0.998 and 0.992), whereas the adsorption isotherm was fitted well by the Freundlich model (R2 = 0.963 and 0.987).					
35680754	0	18	theme	reaction	141:148	arg1	mechanism					150:158	reaction mechanism	141:158	reaction mechanism	141:158	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	2	19	used	used	290:293	arg2	techniques					274:283	Various techniques	266:283	Various techniques	266:283	Various techniques were used to analyze the morphology, structure, and property of the adsorbents, including SEM, EDS, FT-IR, XPS, and zeta potential.					
35680754	3	20	dep	-chitosan	438:446	arg1	III					434:436	III	434:436	III	434:436	Compared with Fe(III)-chitosan, Fe(III)-chitosan-CTAB was more effective for As(V) adsorption at a wide range of pH (3.0-8.0).					
35680754	0	21	theme	-chitosan-CTAB	51:64	arg1	composites					66:75	-chitosan-CTAB composites	51:75	-chitosan-CTAB composites	51:75	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	10	22	theme	different	1490:1498	arg1	results					1517:1523	different characterization results	1490:1523	different characterization results	1490:1523	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	0	23	from	water	100:104	arg1	removal					87:93	As(V) removal	81:93	As(V) removal from water	81:104	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	0	23	from	water	100:104	arg1	V					84:84	V	84:84	V	84:84	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	7	24	theme	initial	1166:1172	arg1	concentration					1174:1186	the initial concentration	1162:1186	the initial concentration of no more than 5 mg L-1	1162:1211	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	7	25	theme	5 mg	1204:1207	arg1	L-1					1209:1211	5 mg L-1	1204:1211	5 mg L-1	1204:1211	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	0	26	theme	comparative	2:12	arg1	study					14:18	A comparative study	0:18	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.	0:159	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	3	27	theme	pH	530:531	arg1	range					521:525	a wide range	514:525	a wide range of pH (3.0-8.0)	514:541	Compared with Fe(III)-chitosan, Fe(III)-chitosan-CTAB was more effective for As(V) adsorption at a wide range of pH (3.0-8.0).					
35680754	6	28	theme	As	995:996	arg1	adsorption					981:990	the adsorption	977:990	the adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB	977:1047	The presence of H2PO4- significantly inhibited the adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB, and humic acid also led to a slight decrease in As(V) adsorption by Fe(III)-chitosan-CTAB.					
35680754	10	29	theme	characterization	1500:1515	arg1	results					1517:1523	different characterization results	1490:1523	different characterization results	1490:1523	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	5	30	dep	described	767:775	arg1	whereas					834:840	whereas	834:840	whereas	834:840	The adsorption kinetics was best described by the pseudo-second-order model (R2 = 0.998 and 0.992), whereas the adsorption isotherm was fitted well by the Freundlich model (R2 = 0.963 and 0.987).					
35680754	7	31	from	concentration	1174:1186	arg1	%					1148:1148	94%	1146:1148	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1	1141:1211	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	7	31	from	concentration	1174:1186	arg1	V					1156:1156	V	1156:1156	V	1156:1156	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	7	31	from	concentration	1174:1186	arg1	As					1153:1154	As	1153:1154	As	1153:1154	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	6	32	from	decrease	1086:1093	arg1	adsorption					1104:1113	As(V) adsorption	1098:1113	As(V) adsorption by Fe(III)-chitosan-CTAB	1098:1138	The presence of H2PO4- significantly inhibited the adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB, and humic acid also led to a slight decrease in As(V) adsorption by Fe(III)-chitosan-CTAB.					
35680754	6	32	from	decrease	1086:1093	arg1	V					1101:1101	V	1101:1101	V	1101:1101	The presence of H2PO4- significantly inhibited the adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB, and humic acid also led to a slight decrease in As(V) adsorption by Fe(III)-chitosan-CTAB.					
35680754	10	33	theme	electrostatic	1531:1543	arg1	attraction					1545:1554	electrostatic attraction	1531:1554	electrostatic attraction	1531:1554	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	0	34	theme	As	81:82	arg1	removal					87:93	As(V) removal	81:93	As(V) removal from water	81:104	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	0	34	theme	As	81:82	arg1	V					84:84	V	84:84	V	84:84	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	5	35	theme	pseudo-second-order	784:802	arg1	model					804:808	the pseudo-second-order model	780:808	the pseudo-second-order model (R2 = 0.998 and 0.992)	780:831	The adsorption kinetics was best described by the pseudo-second-order model (R2 = 0.998 and 0.992), whereas the adsorption isotherm was fitted well by the Freundlich model (R2 = 0.963 and 0.987).					
35680754	3	36	theme	Fe	431:432	arg1	-chitosan					438:446	Fe(III)-chitosan	431:446	Fe(III)-chitosan	431:446	Compared with Fe(III)-chitosan, Fe(III)-chitosan-CTAB was more effective for As(V) adsorption at a wide range of pH (3.0-8.0).					
35680754	7	37	theme	more	1194:1197	arg1	concentration					1174:1186	the initial concentration	1162:1186	the initial concentration of no more than 5 mg L-1	1162:1211	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	6	38	theme	As	1098:1099	arg1	adsorption					1104:1113	As(V) adsorption	1098:1113	As(V) adsorption by Fe(III)-chitosan-CTAB	1098:1138	The presence of H2PO4- significantly inhibited the adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB, and humic acid also led to a slight decrease in As(V) adsorption by Fe(III)-chitosan-CTAB.					
35680754	6	38	theme	As	1098:1099	arg1	V					1101:1101	V	1101:1101	V	1101:1101	The presence of H2PO4- significantly inhibited the adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB, and humic acid also led to a slight decrease in As(V) adsorption by Fe(III)-chitosan-CTAB.					
35680754	1	39	theme	-chitosan-CTAB	189:202	arg1	composites					204:213	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites	161:213	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites	161:213	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites were prepared using an ionotropic gelation method.					
35680754	8	40	theme	1	1264:1264	arg1	%					1265:1265	%	1265:1265	%	1265:1265	1% (w/v) NaOH solution was determined to be the most suitable desorption agent.					
35680754	4	41	dep	-chitosan	580:588	arg1	III					576:578	III	576:578	III	576:578	The adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB could reach equilibrium in 20 min, and their maximum adsorption capacities were 33.85 and 31.69 mg g‒1, respectively.					
35680754	7	42	theme	real	1230:1233	arg1	water					1235:1239	real water	1230:1239	real water	1230:1239	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	3	43	theme	As	494:495	arg1	adsorption					500:509	As(V) adsorption	494:509	As(V) adsorption	494:509	Compared with Fe(III)-chitosan, Fe(III)-chitosan-CTAB was more effective for As(V) adsorption at a wide range of pH (3.0-8.0).					
35680754	3	44	theme	Fe	449:450	arg1	-chitosan-CTAB					456:469	Fe(III)-chitosan-CTAB	449:469	Fe(III)-chitosan-CTAB	449:469	Compared with Fe(III)-chitosan, Fe(III)-chitosan-CTAB was more effective for As(V) adsorption at a wide range of pH (3.0-8.0).					
35680754	9	45	theme	adsorption-desorption	1451:1471	arg1	cycles					1473:1478	five adsorption-desorption cycles	1446:1478	five adsorption-desorption cycles	1446:1478	Fe(III)-chitosan and Fe(III)-chitosan-CTAB still maintained their initial adsorption capacities after five adsorption-desorption cycles.					
35680754	5	46	theme	Freundlich	889:898	arg1	model					900:904	the Freundlich model	885:904	the Freundlich model (R2 = 0.963 and 0.987)	885:927	The adsorption kinetics was best described by the pseudo-second-order model (R2 = 0.998 and 0.992), whereas the adsorption isotherm was fitted well by the Freundlich model (R2 = 0.963 and 0.987).					
35680754	4	47	theme	adsorption	669:678	arg1	capacities					680:689	their maximum adsorption capacities	655:689	their maximum adsorption capacities	655:689	The adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB could reach equilibrium in 20 min, and their maximum adsorption capacities were 33.85 and 31.69 mg g‒1, respectively.					
35680754	7	48	theme	As	1153:1154	arg1	%					1148:1148	94%	1146:1148	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1	1141:1211	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	7	48	theme	As	1153:1154	arg1	V					1156:1156	V	1156:1156	V	1156:1156	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	7	48	theme	As	1153:1154	arg1	As					1153:1154	As	1153:1154	As	1153:1154	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	8	49	theme	suitable	1317:1324	arg1	solution					1278:1285	1% (w/v) NaOH solution	1264:1285	1% (w/v) NaOH solution	1264:1285	1% (w/v) NaOH solution was determined to be the most suitable desorption agent.					
35680754	8	49	theme	suitable	1317:1324	arg1	agent					1337:1341	the most suitable desorption agent	1308:1341	the most suitable desorption agent	1308:1341	1% (w/v) NaOH solution was determined to be the most suitable desorption agent.					
35680754	6	50	dep	Fe	1006:1007	arg1	-chitosan-CTAB					1034:1047	-chitosan-CTAB	1034:1047	-chitosan-CTAB	1034:1047	The presence of H2PO4- significantly inhibited the adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB, and humic acid also led to a slight decrease in As(V) adsorption by Fe(III)-chitosan-CTAB.					
35680754	6	51	theme	slight	1079:1084	arg1	decrease					1086:1093	a slight decrease	1077:1093	a slight decrease in As(V) adsorption by Fe(III)-chitosan-CTAB	1077:1138	The presence of H2PO4- significantly inhibited the adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB, and humic acid also led to a slight decrease in As(V) adsorption by Fe(III)-chitosan-CTAB.					
35680754	6	52	theme	humic	1054:1058	arg1	acid					1060:1063	humic acid	1054:1063	humic acid	1054:1063	The presence of H2PO4- significantly inhibited the adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB, and humic acid also led to a slight decrease in As(V) adsorption by Fe(III)-chitosan-CTAB.					
35680754	7	53	attach	removed	1217:1223	arg2	V					1156:1156	V	1156:1156	V	1156:1156	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	7	53	attach	removed	1217:1223	arg1	water					1235:1239	real water	1230:1239	real water	1230:1239	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	7	53	attach	removed	1217:1223	arg2	As					1153:1154	As	1153:1154	As	1153:1154	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	7	53	attach	removed	1217:1223	arg2	%					1148:1148	94%	1146:1148	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1	1141:1211	Over 94% of As(V) at the initial concentration of no more than 5 mg L-1 was removed from real water by the two adsorbents.					
35680754	3	54	from	range	521:525	arg1	effective					480:488	effective	480:488	effective	480:488	Compared with Fe(III)-chitosan, Fe(III)-chitosan-CTAB was more effective for As(V) adsorption at a wide range of pH (3.0-8.0).					
35680754	4	55	theme	Fe	594:595	arg1	-chitosan-CTAB					601:614	Fe(III)-chitosan-CTAB	594:614	Fe(III)-chitosan-CTAB	594:614	The adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB could reach equilibrium in 20 min, and their maximum adsorption capacities were 33.85 and 31.69 mg g‒1, respectively.					
35680754	3	56	theme	wide	516:519	arg1	range					521:525	a wide range	514:525	a wide range of pH (3.0-8.0)	514:541	Compared with Fe(III)-chitosan, Fe(III)-chitosan-CTAB was more effective for As(V) adsorption at a wide range of pH (3.0-8.0).					
35680754	0	57	dep	Fe	23:24	arg1	composites					66:75	-chitosan-CTAB composites	51:75	-chitosan-CTAB composites	51:75	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	8	58	dep	%	1265:1265	arg1	w/v					1268:1270	w/v	1268:1270	w/v	1268:1270	1% (w/v) NaOH solution was determined to be the most suitable desorption agent.					
35680754	10	59	theme	As	1618:1619	arg1	adsorption					1624:1633	As(V) adsorption	1618:1633	As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB	1618:1679	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	10	59	theme	As	1618:1619	arg1	V					1621:1621	V	1621:1621	V	1621:1621	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	10	60	dep	Fe	1638:1639	arg1	-chitosan					1645:1653	-chitosan	1645:1653	-chitosan	1645:1653	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	6	61	theme	H2PO4-	946:951	arg1	presence					934:941	The presence	930:941	The presence of H2PO4-	930:951	The presence of H2PO4- significantly inhibited the adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB, and humic acid also led to a slight decrease in As(V) adsorption by Fe(III)-chitosan-CTAB.					
35680754	5	62	theme	adsorption	738:747	arg1	kinetics					749:756	The adsorption kinetics	734:756	The adsorption kinetics	734:756	The adsorption kinetics was best described by the pseudo-second-order model (R2 = 0.998 and 0.992), whereas the adsorption isotherm was fitted well by the Freundlich model (R2 = 0.963 and 0.987).					
35680754	8	63	theme	desorption	1326:1335	arg1	solution					1278:1285	1% (w/v) NaOH solution	1264:1285	1% (w/v) NaOH solution	1264:1285	1% (w/v) NaOH solution was determined to be the most suitable desorption agent.					
35680754	8	63	theme	desorption	1326:1335	arg1	agent					1337:1341	the most suitable desorption agent	1308:1341	the most suitable desorption agent	1308:1341	1% (w/v) NaOH solution was determined to be the most suitable desorption agent.					
35680754	1	64	theme	Fe	161:162	arg1	-chitosan					168:176	Fe(III)-chitosan	161:176	Fe(III)-chitosan	161:176	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites were prepared using an ionotropic gelation method.					
35680754	10	65	dep	Fe	1659:1660	arg1	-chitosan-CTAB					1666:1679	-chitosan-CTAB	1666:1679	Fe(III)-chitosan-CTAB	1659:1679	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	1	66	dep	-chitosan	168:176	arg1	III					164:166	III	164:166	III	164:166	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites were prepared using an ionotropic gelation method.					
35680754	0	67	dep	study	14:18	arg1	characterization					120:135	characterization	120:135	characterization	120:135	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	0	67	dep	study	14:18	arg1	mechanism					150:158	reaction mechanism	141:158	reaction mechanism	141:158	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	0	67	dep	study	14:18	arg1	preparation					107:117	preparation	107:117	preparation	107:117	A comparative study on Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites for As(V) removal from water: preparation, characterization and reaction mechanism.					
35680754	4	68	theme	maximum	661:667	arg1	capacities					680:689	their maximum adsorption capacities	655:689	their maximum adsorption capacities	655:689	The adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB could reach equilibrium in 20 min, and their maximum adsorption capacities were 33.85 and 31.69 mg g‒1, respectively.					
35680754	9	69	theme	adsorption	1418:1427	arg1	capacities					1429:1438	their initial adsorption capacities	1404:1438	their initial adsorption capacities	1404:1438	Fe(III)-chitosan and Fe(III)-chitosan-CTAB still maintained their initial adsorption capacities after five adsorption-desorption cycles.					
35680754	9	70	theme	initial	1410:1416	arg1	capacities					1429:1438	their initial adsorption capacities	1404:1438	their initial adsorption capacities	1404:1438	Fe(III)-chitosan and Fe(III)-chitosan-CTAB still maintained their initial adsorption capacities after five adsorption-desorption cycles.					
35680754	10	71	theme	important	1599:1607	arg1	roles					1609:1613	important roles	1599:1613	important roles	1599:1613	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35680754	5	72	dep	model	804:808	arg1	R2 = 0.998					811:820	R2 = 0.998	811:820	R2 = 0.998	811:820	The adsorption kinetics was best described by the pseudo-second-order model (R2 = 0.998 and 0.992), whereas the adsorption isotherm was fitted well by the Freundlich model (R2 = 0.963 and 0.987).					
35680754	5	72	dep	model	804:808	arg1	0.992					826:830	0.992	826:830	0.992	826:830	The adsorption kinetics was best described by the pseudo-second-order model (R2 = 0.998 and 0.992), whereas the adsorption isotherm was fitted well by the Freundlich model (R2 = 0.963 and 0.987).					
35680754	1	73	theme	ionotropic	238:247	arg1	method					258:263	an ionotropic gelation method	235:263	an ionotropic gelation method	235:263	Fe(III)-chitosan and Fe(III)-chitosan-CTAB composites were prepared using an ionotropic gelation method.					
35680754	4	74	theme	Fe	573:574	arg1	-chitosan					580:588	Fe(III)-chitosan	573:588	Fe(III)-chitosan	573:588	The adsorption of As(V) onto Fe(III)-chitosan and Fe(III)-chitosan-CTAB could reach equilibrium in 20 min, and their maximum adsorption capacities were 33.85 and 31.69 mg g‒1, respectively.					
35680754	2	75	theme	adsorbents	353:362	arg1	structure					322:330	structure	322:330	structure	322:330	Various techniques were used to analyze the morphology, structure, and property of the adsorbents, including SEM, EDS, FT-IR, XPS, and zeta potential.					
35680754	2	75	theme	adsorbents	353:362	arg1	property					337:344	property	337:344	property	337:344	Various techniques were used to analyze the morphology, structure, and property of the adsorbents, including SEM, EDS, FT-IR, XPS, and zeta potential.					
35680754	2	75	theme	adsorbents	353:362	arg1	morphology					310:319	morphology	310:319	morphology	310:319	Various techniques were used to analyze the morphology, structure, and property of the adsorbents, including SEM, EDS, FT-IR, XPS, and zeta potential.					
35680754	8	76	theme	%	1265:1265	arg1	solution					1278:1285	1% (w/v) NaOH solution	1264:1285	1% (w/v) NaOH solution	1264:1285	1% (w/v) NaOH solution was determined to be the most suitable desorption agent.					
35680754	8	76	theme	%	1265:1265	arg1	agent					1337:1341	the most suitable desorption agent	1308:1341	the most suitable desorption agent	1308:1341	1% (w/v) NaOH solution was determined to be the most suitable desorption agent.					
35680754	10	77	theme	complexation	1568:1579	arg1	mechanisms					1581:1590	surface complexation mechanisms	1560:1590	surface complexation mechanisms	1560:1590	Based on different characterization results, both electrostatic attraction and surface complexation mechanisms played important roles in As(V) adsorption on Fe(III)-chitosan and Fe(III)-chitosan-CTAB.					
35728476	3	0	theme	organic	658:664	arg1	pollutants					666:675	organic pollutants	658:675	organic pollutants	658:675	The performance of biomass and red mud based magnetic adsorbents is also evaluated, and their primary adsorption mechanisms for organic pollutants are revealed by using different organic model compounds.					
35728476	5	1	theme	pine	845:848	arg1	800 °C					858:863	pine wood at 800 °C	845:863	pine wood at 800 °C	845:863	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	7	2	with	co-pyrolysis	1174:1185	arg1	mud					1207:1209	red mud	1203:1209	red mud	1203:1209	The findings show that co-pyrolysis of biomass with red mud can reduce waste while also producing a flexible adsorbent that is magnetically separable and effective at absorbing different organic contaminants from water.					
35728476	0	3	theme	waste	69:73	arg1	co-pyrolysis					43:54	co-pyrolysis	43:54	co-pyrolysis of pine wood waste and red mud	43:85	Production of a bio-magnetic adsorbent via co-pyrolysis of pine wood waste and red mud.					
35728476	3	4	theme	adsorption	632:641	arg1	mechanisms					643:652	their primary adsorption mechanisms	618:652	their primary adsorption mechanisms for organic pollutants	618:675	The performance of biomass and red mud based magnetic adsorbents is also evaluated, and their primary adsorption mechanisms for organic pollutants are revealed by using different organic model compounds.					
35728476	2	5	theme	co-pyrolysis	331:342	arg1	mechanism					318:326	the reaction mechanism	305:326	the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles	305:527	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	0	6	theme	mud	83:85	arg1	co-pyrolysis					43:54	co-pyrolysis	43:54	co-pyrolysis of pine wood waste and red mud	43:85	Production of a bio-magnetic adsorbent via co-pyrolysis of pine wood waste and red mud.					
35728476	7	7	theme	different	1328:1336	arg1	contaminants					1346:1357	different organic contaminants	1328:1357	different organic contaminants from water	1328:1368	The findings show that co-pyrolysis of biomass with red mud can reduce waste while also producing a flexible adsorbent that is magnetically separable and effective at absorbing different organic contaminants from water.					
35728476	7	8	theme	organic	1338:1344	arg1	contaminants					1346:1357	different organic contaminants	1328:1357	different organic contaminants from water	1328:1368	The findings show that co-pyrolysis of biomass with red mud can reduce waste while also producing a flexible adsorbent that is magnetically separable and effective at absorbing different organic contaminants from water.					
35728476	5	9	theme	ibuprofen	907:915	arg1	capacity					895:902	the highest adsorption capacity	872:902	the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5	872:948	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	0	10	theme	red	79:81	arg1	mud					83:85	red mud	79:85	red mud	79:85	Production of a bio-magnetic adsorbent via co-pyrolysis of pine wood waste and red mud.					
35728476	7	11	theme	red	1203:1205	arg1	mud					1207:1209	red mud	1203:1209	red mud	1203:1209	The findings show that co-pyrolysis of biomass with red mud can reduce waste while also producing a flexible adsorbent that is magnetically separable and effective at absorbing different organic contaminants from water.					
35728476	5	12	from	∼pH	942:944	arg1	21.01 mg/g					928:937	21.01 mg/g	928:937	21.01 mg/g	928:937	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	5	13	theme	at	855:856	arg1	800 °C					858:863	pine wood at 800 °C	845:863	pine wood at 800 °C	845:863	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	6	14	theme	adsorption	1130:1139	arg1	efficacy					1141:1148	greater adsorption efficacy	1122:1148	greater adsorption efficacy	1122:1148	When compared to lignin-rich biomass, adsorbents composed of cellulose-rich biomass showed greater adsorption efficacy.					
35728476	1	15	theme	mud	149:151	arg1	difficult					218:226	difficult	218:226	difficult	218:226	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	1	15	theme	mud	149:151	arg1	reduction					102:110	The efficient reduction	88:110	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent	88:208	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	5	16	theme	wood	850:853	arg1	800 °C					858:863	pine wood at 800 °C	845:863	pine wood at 800 °C	845:863	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	6	17	theme	greater	1122:1128	arg1	efficacy					1141:1148	greater adsorption efficacy	1122:1148	greater adsorption efficacy	1122:1148	When compared to lignin-rich biomass, adsorbents composed of cellulose-rich biomass showed greater adsorption efficacy.					
35728476	2	18	theme	pine	378:381	arg1	wood					383:386	pine wood	378:386	pine wood	378:386	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	19	theme	red	417:419	arg1	mud					421:423	red mud	417:423	red mud at 500, 650, and 800 °C	417:447	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	20	from	500	428:430	arg1	mud					421:423	red mud	417:423	red mud at 500, 650, and 800 °C	417:447	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	7	21	theme	biomass	1190:1196	arg1	co-pyrolysis					1174:1185	co-pyrolysis	1174:1185	co-pyrolysis of biomass with red mud	1174:1209	The findings show that co-pyrolysis of biomass with red mud can reduce waste while also producing a flexible adsorbent that is magnetically separable and effective at absorbing different organic contaminants from water.					
35728476	5	22	theme	highest	876:882	arg1	capacity					895:902	the highest adsorption capacity	872:902	the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5	872:948	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	3	23	theme	organic	709:715	arg1	compounds					723:731	different organic model compounds	699:731	different organic model compounds	699:731	The performance of biomass and red mud based magnetic adsorbents is also evaluated, and their primary adsorption mechanisms for organic pollutants are revealed by using different organic model compounds.					
35728476	5	24	theme	adsorption	884:893	arg1	capacity					895:902	the highest adsorption capacity	872:902	the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5	872:948	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	5	25	theme	adsorption	1009:1018	arg1	mechanism					1020:1028	the dominant adsorption mechanism	996:1028	the dominant adsorption mechanism	996:1028	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	5	25	theme	adsorption	1009:1018	arg1	interactions					980:991	strong π stacking interactions	962:991	strong π stacking interactions	962:991	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	1	26	theme	sustainability	253:266	arg1	terms					244:248	terms	244:248	terms of sustainability	244:266	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	2	27	theme	produced	497:504	arg1	particles					519:527	the produced bio-magnetic particles	493:527	the produced bio-magnetic particles	493:527	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	5	28	theme	π	969:969	arg1	mechanism					1020:1028	the dominant adsorption mechanism	996:1028	the dominant adsorption mechanism	996:1028	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	5	28	theme	π	969:969	arg1	interactions					980:991	strong π stacking interactions	962:991	strong π stacking interactions	962:991	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	6	29	theme	cellulose-rich	1092:1105	arg1	biomass					1107:1113	cellulose-rich biomass	1092:1113	cellulose-rich biomass	1092:1113	When compared to lignin-rich biomass, adsorbents composed of cellulose-rich biomass showed greater adsorption efficacy.					
35728476	4	30	theme	best	776:779	arg1	performance					781:791	the best performance	772:791	the best performance	772:791	The samples produced at 800 °C showed the best performance.					
35728476	7	31	from	water	1364:1368	arg1	contaminants					1346:1357	different organic contaminants	1328:1357	different organic contaminants from water	1328:1368	The findings show that co-pyrolysis of biomass with red mud can reduce waste while also producing a flexible adsorbent that is magnetically separable and effective at absorbing different organic contaminants from water.					
35728476	1	32	from	terms	244:248	arg1	difficult					218:226	difficult	218:226	difficult	218:226	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	1	32	from	terms	244:248	arg1	reduction					102:110	The efficient reduction	88:110	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent	88:208	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	5	33	theme	dominant	1000:1007	arg1	mechanism					1020:1028	the dominant adsorption mechanism	996:1028	the dominant adsorption mechanism	996:1028	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	5	33	theme	dominant	1000:1007	arg1	interactions					980:991	strong π stacking interactions	962:991	strong π stacking interactions	962:991	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	1	34	theme	red	145:147	arg1	mud					149:151	red mud	145:151	red mud	145:151	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	1	35	theme	waste	127:131	arg1	biomass					133:139	accumulated waste biomass	115:139	accumulated waste biomass	115:139	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	3	36	theme	different	699:707	arg1	compounds					723:731	different organic model compounds	699:731	different organic model compounds	699:731	The performance of biomass and red mud based magnetic adsorbents is also evaluated, and their primary adsorption mechanisms for organic pollutants are revealed by using different organic model compounds.					
35728476	2	37	from	650	433:435	arg1	mud					421:423	red mud	417:423	red mud at 500, 650, and 800 °C	417:447	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	3	38	theme	biomass	549:555	arg1	adsorbents					584:593	biomass and red mud based magnetic adsorbents	549:593	biomass and red mud based magnetic adsorbents	549:593	The performance of biomass and red mud based magnetic adsorbents is also evaluated, and their primary adsorption mechanisms for organic pollutants are revealed by using different organic model compounds.					
35728476	5	39	theme	red	833:835	arg1	mud					837:839	red mud	833:839	red mud	833:839	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	0	40	theme	adsorbent	29:37	arg1	Production					0:9	Production	0:9	Production of a bio-magnetic adsorbent via co-pyrolysis of pine wood waste and red mud.	0:86	Production of a bio-magnetic adsorbent via co-pyrolysis of pine wood waste and red mud.					
35728476	2	41	theme	characterizations	472:488	arg1	co-pyrolysis					331:342	co-pyrolysis	331:342	co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles	331:527	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	42	from	800 °C	442:447	arg1	mud					421:423	red mud	417:423	red mud at 500, 650, and 800 °C	417:447	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	43	theme	comprehensive	458:470	arg1	cellulose					389:397	cellulose	389:397	cellulose	389:397	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	43	theme	comprehensive	458:470	arg1	characterizations					472:488	the comprehensive characterizations	454:488	the comprehensive characterizations of the produced bio-magnetic particles	454:527	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	43	theme	comprehensive	458:470	arg1	wood					383:386	pine wood	378:386	pine wood	378:386	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	43	theme	comprehensive	458:470	arg1	lignin					404:409	lignin	404:409	lignin	404:409	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	3	44	theme	model	717:721	arg1	compounds					723:731	different organic model compounds	699:731	different organic model compounds	699:731	The performance of biomass and red mud based magnetic adsorbents is also evaluated, and their primary adsorption mechanisms for organic pollutants are revealed by using different organic model compounds.					
35728476	5	45	theme	strong	962:967	arg1	mechanism					1020:1028	the dominant adsorption mechanism	996:1028	the dominant adsorption mechanism	996:1028	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	5	45	theme	strong	962:967	arg1	interactions					980:991	strong π stacking interactions	962:991	strong π stacking interactions	962:991	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	6	46	theme	lignin-rich	1048:1058	arg1	biomass					1060:1066	lignin-rich biomass	1048:1066	lignin-rich biomass	1048:1066	When compared to lignin-rich biomass, adsorbents composed of cellulose-rich biomass showed greater adsorption efficacy.					
35728476	1	47	theme	efficient	92:100	arg1	difficult					218:226	difficult	218:226	difficult	218:226	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	1	47	theme	efficient	92:100	arg1	reduction					102:110	The efficient reduction	88:110	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent	88:208	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	1	48	from	difficult	218:226	arg1	terms					244:248	terms	244:248	terms of sustainability	244:266	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	5	49	theme	stacking	971:978	arg1	mechanism					1020:1028	the dominant adsorption mechanism	996:1028	the dominant adsorption mechanism	996:1028	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	5	49	theme	stacking	971:978	arg1	interactions					980:991	strong π stacking interactions	962:991	strong π stacking interactions	962:991	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	1	50	theme	biomass	133:139	arg1	difficult					218:226	difficult	218:226	difficult	218:226	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	1	50	theme	biomass	133:139	arg1	reduction					102:110	The efficient reduction	88:110	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent	88:208	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	3	51	theme	adsorbents	584:593	arg1	performance					534:544	The performance	530:544	The performance of biomass and red mud based magnetic adsorbents	530:593	The performance of biomass and red mud based magnetic adsorbents is also evaluated, and their primary adsorption mechanisms for organic pollutants are revealed by using different organic model compounds.					
35728476	5	52	from	800 °C	858:863	arg1	pine					845:848	pine wood at 800 °C	845:863	pine wood at 800 °C	845:863	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	5	52	from	800 °C	858:863	arg1	wood					850:853	pine wood at 800 °C	845:863	pine wood at 800 °C	845:863	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	5	52	from	800 °C	858:863	arg1	at					855:856	pine wood at 800 °C	845:863	pine wood at 800 °C	845:863	For example, the sample prepared using red mud and pine wood at 800 °C showed the highest adsorption capacity of ibuprofen, which was 21.01 mg/g at ∼pH 4.5, indicating strong π stacking interactions as the dominant adsorption mechanism.					
35728476	2	53	theme	particles	519:527	arg1	cellulose					389:397	cellulose	389:397	cellulose	389:397	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	53	theme	particles	519:527	arg1	characterizations					472:488	the comprehensive characterizations	454:488	the comprehensive characterizations of the produced bio-magnetic particles	454:527	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	53	theme	particles	519:527	arg1	wood					383:386	pine wood	378:386	pine wood	378:386	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	53	theme	particles	519:527	arg1	lignin					404:409	lignin	404:409	lignin	404:409	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	53	theme	particles	519:527	arg1	biomasses					357:365	different biomasses	347:365	different biomasses	347:365	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	3	54	theme	primary	624:630	arg1	mechanisms					643:652	their primary adsorption mechanisms	618:652	their primary adsorption mechanisms for organic pollutants	618:675	The performance of biomass and red mud based magnetic adsorbents is also evaluated, and their primary adsorption mechanisms for organic pollutants are revealed by using different organic model compounds.					
35728476	3	55	theme	based	569:573	arg1	adsorbents					584:593	biomass and red mud based magnetic adsorbents	549:593	biomass and red mud based magnetic adsorbents	549:593	The performance of biomass and red mud based magnetic adsorbents is also evaluated, and their primary adsorption mechanisms for organic pollutants are revealed by using different organic model compounds.					
35728476	2	56	theme	reaction	309:316	arg1	mechanism					318:326	the reaction mechanism	305:326	the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles	305:527	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	57	with	characterizations	472:488	arg1	mud					421:423	red mud	417:423	red mud at 500, 650, and 800 °C	417:447	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	58	theme	bio-magnetic	506:517	arg1	particles					519:527	the produced bio-magnetic particles	493:527	the produced bio-magnetic particles	493:527	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	59	with	biomasses	357:365	arg1	mud					421:423	red mud	417:423	red mud at 500, 650, and 800 °C	417:447	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	3	60	theme	magnetic	575:582	arg1	adsorbents					584:593	biomass and red mud based magnetic adsorbents	549:593	biomass and red mud based magnetic adsorbents	549:593	The performance of biomass and red mud based magnetic adsorbents is also evaluated, and their primary adsorption mechanisms for organic pollutants are revealed by using different organic model compounds.					
35728476	1	61	theme	accumulated	115:125	arg1	biomass					133:139	accumulated waste biomass	115:139	accumulated waste biomass	115:139	The efficient reduction of accumulated waste biomass and red mud by converting them into a value-added magnetic adsorbent is both difficult and tempting in terms of sustainability.					
35728476	0	62	theme	wood	64:67	arg1	waste					69:73	pine wood waste	59:73	pine wood waste	59:73	Production of a bio-magnetic adsorbent via co-pyrolysis of pine wood waste and red mud.					
35728476	3	63	theme	red	561:563	arg1	mud					565:567	red mud	561:567	red mud	561:567	The performance of biomass and red mud based magnetic adsorbents is also evaluated, and their primary adsorption mechanisms for organic pollutants are revealed by using different organic model compounds.					
35728476	2	64	theme	biomasses	357:365	arg1	co-pyrolysis					331:342	co-pyrolysis	331:342	co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles	331:527	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	0	65	theme	pine	59:62	arg1	waste					69:73	pine wood waste	59:73	pine wood waste	59:73	Production of a bio-magnetic adsorbent via co-pyrolysis of pine wood waste and red mud.					
35728476	3	66	theme	mud	565:567	arg1	adsorbents					584:593	biomass and red mud based magnetic adsorbents	549:593	biomass and red mud based magnetic adsorbents	549:593	The performance of biomass and red mud based magnetic adsorbents is also evaluated, and their primary adsorption mechanisms for organic pollutants are revealed by using different organic model compounds.					
35728476	2	67	theme	different	347:355	arg1	cellulose					389:397	cellulose	389:397	cellulose	389:397	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	67	theme	different	347:355	arg1	lignin					404:409	lignin	404:409	lignin	404:409	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	67	theme	different	347:355	arg1	wood					383:386	pine wood	378:386	pine wood	378:386	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35728476	2	67	theme	different	347:355	arg1	biomasses					357:365	different biomasses	347:365	different biomasses	347:365	This study focused on investigating the reaction mechanism of co-pyrolysis of different biomasses, including pine wood, cellulose, and lignin, with red mud at 500, 650, and 800 °C, and the comprehensive characterizations of the produced bio-magnetic particles.					
35151706	0	0	theme	glycol	75:80	arg1	scaffolds					82:90	3D printed sodium alginate/polyethylene glycol scaffolds	35:90	3D printed sodium alginate/polyethylene glycol scaffolds	35:90	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	4	1	theme	kinetic	881:887	arg1	model					889:893	Hixson-Crowell kinetic model	866:893	Hixson-Crowell kinetic model	866:893	Furthermore, insulin-loaded 9SA/3PEG scaffolds showed ultrafast wetting (<1 s), disintegration (<6 s), and also dissolution (<30 s) according to Hixson-Crowell kinetic model.					
35151706	2	2	theme	sodium	378:383	arg1	PEG					420:422	PEG	420:422	PEG	420:422	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	2	2	theme	sodium	378:383	arg1	glycol					412:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol	356:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds	356:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	9	3	theme	delivery	1544:1551	arg1	strategy					1553:1560	a new promising delivery strategy	1528:1560	a new promising delivery strategy for administering injectable insulin through the sublingual route	1528:1626	The polymeric scaffold with a high safety and efficacy holds a new promising delivery strategy for administering injectable insulin through the sublingual route.					
35151706	0	4	theme	alginate/polyethylene	53:73	arg1	scaffolds					82:90	3D printed sodium alginate/polyethylene glycol scaffolds	35:90	3D printed sodium alginate/polyethylene glycol scaffolds	35:90	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	4	5	theme	Hixson-Crowell	866:879	arg1	model					889:893	Hixson-Crowell kinetic model	866:893	Hixson-Crowell kinetic model	866:893	Furthermore, insulin-loaded 9SA/3PEG scaffolds showed ultrafast wetting (<1 s), disintegration (<6 s), and also dissolution (<30 s) according to Hixson-Crowell kinetic model.					
35151706	5	6	from	viability	905:913	arg1	scaffolds					943:951	3D printed scaffolds	932:951	3D printed scaffolds	932:951	The cell viability of L929 cells on 3D printed scaffolds was examined and these scaffolds could be safely applied on animals.					
35151706	2	7	theme	sublingual	367:376	arg1	PEG					420:422	PEG	420:422	PEG	420:422	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	2	7	theme	sublingual	367:376	arg1	glycol					412:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol	356:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds	356:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	9	8	theme	high	1497:1500	arg1	safety					1502:1507	a high safety	1495:1507	a high safety	1495:1507	The polymeric scaffold with a high safety and efficacy holds a new promising delivery strategy for administering injectable insulin through the sublingual route.					
35151706	1	9	theme	3D	269:270	arg1	candidates					304:313	potential candidates	294:313	potential candidates as carriers	294:325	Delivery of therapeutic peptides via sublingual administration is extremely desired and 3D printed scaffolds are potential candidates as carriers to enhance insulin delivery.					
35151706	1	9	theme	3D	269:270	arg1	scaffolds					280:288	3D printed scaffolds	269:288	3D printed scaffolds	269:288	Delivery of therapeutic peptides via sublingual administration is extremely desired and 3D printed scaffolds are potential candidates as carriers to enhance insulin delivery.					
35151706	3	10	theme	insulin	712:718	arg1	structure					699:707	the crystalline structure	683:707	the crystalline structure of insulin	683:718	The tensile strength and flexibility of scaffolds increased after loading insulin due to the crystalline structure of insulin.					
35151706	5	11	theme	cell	900:903	arg1	viability					905:913	The cell viability	896:913	The cell viability of L929 cells on 3D printed scaffolds	896:951	The cell viability of L929 cells on 3D printed scaffolds was examined and these scaffolds could be safely applied on animals.					
35151706	1	12	theme	printed	272:278	arg1	candidates					304:313	potential candidates	294:313	potential candidates as carriers	294:325	Delivery of therapeutic peptides via sublingual administration is extremely desired and 3D printed scaffolds are potential candidates as carriers to enhance insulin delivery.					
35151706	1	12	theme	printed	272:278	arg1	scaffolds					280:288	3D printed scaffolds	269:288	3D printed scaffolds	269:288	Delivery of therapeutic peptides via sublingual administration is extremely desired and 3D printed scaffolds are potential candidates as carriers to enhance insulin delivery.					
35151706	0	13	theme	scaffolds	82:90	arg1	evaluation					21:30	In vitro and in vivo evaluation	0:30	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.	0:179	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	9	14	theme	new	1530:1532	arg1	strategy					1553:1560	a new promising delivery strategy	1528:1560	a new promising delivery strategy for administering injectable insulin through the sublingual route	1528:1626	The polymeric scaffold with a high safety and efficacy holds a new promising delivery strategy for administering injectable insulin through the sublingual route.					
35151706	9	15	theme	polymeric	1471:1479	arg1	scaffold					1481:1488	The polymeric scaffold	1467:1488	The polymeric scaffold with a high safety and efficacy	1467:1520	The polymeric scaffold with a high safety and efficacy holds a new promising delivery strategy for administering injectable insulin through the sublingual route.					
35151706	4	16	theme	9SA/3PEG	749:756	arg1	scaffolds					758:766	insulin-loaded 9SA/3PEG scaffolds	734:766	insulin-loaded 9SA/3PEG scaffolds	734:766	Furthermore, insulin-loaded 9SA/3PEG scaffolds showed ultrafast wetting (<1 s), disintegration (<6 s), and also dissolution (<30 s) according to Hixson-Crowell kinetic model.					
35151706	7	17	theme	hypoglycemic	1207:1218	arg1	effect					1220:1225	a longer hypoglycemic effect	1198:1225	a longer hypoglycemic effect	1198:1225	A single dose of scaffold presented a longer hypoglycemic effect, reducing ~60% of glycemia after 30 min and it lasted for 12 h by increasing the bioavailability of insulin.					
35151706	4	18	theme	insulin-loaded	734:747	arg1	scaffolds					758:766	insulin-loaded 9SA/3PEG scaffolds	734:766	insulin-loaded 9SA/3PEG scaffolds	734:766	Furthermore, insulin-loaded 9SA/3PEG scaffolds showed ultrafast wetting (<1 s), disintegration (<6 s), and also dissolution (<30 s) according to Hixson-Crowell kinetic model.					
35151706	6	19	theme	glucose	1059:1065	arg1	level					1067:1071	blood glucose level	1053:1071	blood glucose level	1053:1071	Pharmacokinetic parameters and blood glucose level were evaluated following sublingual administration of scaffolds to type-1 diabetic rats.					
35151706	7	20	theme	insulin	1327:1333	arg1	bioavailability					1308:1322	the bioavailability	1304:1322	the bioavailability of insulin	1304:1333	A single dose of scaffold presented a longer hypoglycemic effect, reducing ~60% of glycemia after 30 min and it lasted for 12 h by increasing the bioavailability of insulin.					
35151706	2	21	theme	glycol	412:417	arg1	scaffolds					435:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds	356:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds	356:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	0	22	theme	sublingual	96:105	arg1	delivery					107:114	sublingual delivery	96:114	sublingual delivery of insulin	96:125	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	5	23	theme	printed	935:941	arg1	scaffolds					943:951	3D printed scaffolds	932:951	3D printed scaffolds	932:951	The cell viability of L929 cells on 3D printed scaffolds was examined and these scaffolds could be safely applied on animals.					
35151706	2	24	theme	/polyethylene	398:410	arg1	PEG					420:422	PEG	420:422	PEG	420:422	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	2	24	theme	/polyethylene	398:410	arg1	glycol					412:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol	356:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds	356:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	4	25	theme	ultrafast	775:783	arg1	<1 s					794:797	ultrafast wetting (<1 s)	775:798	ultrafast wetting (<1 s)	775:798	Furthermore, insulin-loaded 9SA/3PEG scaffolds showed ultrafast wetting (<1 s), disintegration (<6 s), and also dissolution (<30 s) according to Hixson-Crowell kinetic model.					
35151706	3	26	dep	strength	606:613	arg1	The					594:596	The	594:596	The	594:596	The tensile strength and flexibility of scaffolds increased after loading insulin due to the crystalline structure of insulin.					
35151706	0	27	theme	In	0:1	arg1	evaluation					21:30	In vitro and in vivo evaluation	0:30	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.	0:179	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	9	28	theme	injectable	1580:1589	arg1	insulin					1591:1597	injectable insulin	1580:1597	injectable insulin	1580:1597	The polymeric scaffold with a high safety and efficacy holds a new promising delivery strategy for administering injectable insulin through the sublingual route.					
35151706	3	29	theme	crystalline	687:697	arg1	structure					699:707	the crystalline structure	683:707	the crystalline structure of insulin	683:718	The tensile strength and flexibility of scaffolds increased after loading insulin due to the crystalline structure of insulin.					
35151706	9	30	with	scaffold	1481:1488	arg1	efficacy					1513:1520	efficacy	1513:1520	efficacy	1513:1520	The polymeric scaffold with a high safety and efficacy holds a new promising delivery strategy for administering injectable insulin through the sublingual route.					
35151706	9	30	with	scaffold	1481:1488	arg1	safety					1502:1507	a high safety	1495:1507	a high safety	1495:1507	The polymeric scaffold with a high safety and efficacy holds a new promising delivery strategy for administering injectable insulin through the sublingual route.					
35151706	2	31	theme	SA	395:396	arg1	PEG					420:422	PEG	420:422	PEG	420:422	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	2	31	theme	SA	395:396	arg1	glycol					412:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol	356:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds	356:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	0	32	theme	in	13:14	arg1	evaluation					21:30	In vitro and in vivo evaluation	0:30	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.	0:179	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	1	33	theme	potential	294:302	arg1	candidates					304:313	potential candidates	294:313	potential candidates as carriers	294:325	Delivery of therapeutic peptides via sublingual administration is extremely desired and 3D printed scaffolds are potential candidates as carriers to enhance insulin delivery.					
35151706	1	33	theme	potential	294:302	arg1	scaffolds					280:288	3D printed scaffolds	269:288	3D printed scaffolds	269:288	Delivery of therapeutic peptides via sublingual administration is extremely desired and 3D printed scaffolds are potential candidates as carriers to enhance insulin delivery.					
35151706	0	34	theme	insulin	119:125	arg1	delivery					107:114	sublingual delivery	96:114	sublingual delivery of insulin	96:125	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	5	35	theme	3D	932:933	arg1	scaffolds					943:951	3D printed scaffolds	932:951	3D printed scaffolds	932:951	The cell viability of L929 cells on 3D printed scaffolds was examined and these scaffolds could be safely applied on animals.					
35151706	1	36	theme	therapeutic	193:203	arg1	peptides					205:212	therapeutic peptides	193:212	therapeutic peptides	193:212	Delivery of therapeutic peptides via sublingual administration is extremely desired and 3D printed scaffolds are potential candidates as carriers to enhance insulin delivery.					
35151706	6	37	theme	type-1	1140:1145	arg1	rats					1156:1159	type-1 diabetic rats	1140:1159	type-1 diabetic rats	1140:1159	Pharmacokinetic parameters and blood glucose level were evaluated following sublingual administration of scaffolds to type-1 diabetic rats.					
35151706	2	38	theme	cytotoxic	553:561	arg1	features					584:591	the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features	503:591	the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features	503:591	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	4	39	theme	wetting	785:791	arg1	<1 s					794:797	ultrafast wetting (<1 s)	775:798	ultrafast wetting (<1 s)	775:798	Furthermore, insulin-loaded 9SA/3PEG scaffolds showed ultrafast wetting (<1 s), disintegration (<6 s), and also dissolution (<30 s) according to Hixson-Crowell kinetic model.					
35151706	9	40	theme	promising	1534:1542	arg1	strategy					1553:1560	a new promising delivery strategy	1528:1560	a new promising delivery strategy for administering injectable insulin through the sublingual route	1528:1626	The polymeric scaffold with a high safety and efficacy holds a new promising delivery strategy for administering injectable insulin through the sublingual route.					
35151706	1	41	theme	peptides	205:212	arg1	Delivery					181:188	Delivery	181:188	Delivery of therapeutic peptides via sublingual administration	181:242	Delivery of therapeutic peptides via sublingual administration is extremely desired and 3D printed scaffolds are potential candidates as carriers to enhance insulin delivery.					
35151706	5	42	theme	cells	923:927	arg1	viability					905:913	The cell viability	896:913	The cell viability of L929 cells on 3D printed scaffolds	896:951	The cell viability of L929 cells on 3D printed scaffolds was examined and these scaffolds could be safely applied on animals.					
35151706	7	43	theme	longer	1200:1205	arg1	effect					1220:1225	a longer hypoglycemic effect	1198:1225	a longer hypoglycemic effect	1198:1225	A single dose of scaffold presented a longer hypoglycemic effect, reducing ~60% of glycemia after 30 min and it lasted for 12 h by increasing the bioavailability of insulin.					
35151706	9	44	theme	sublingual	1611:1620	arg1	route					1622:1626	the sublingual route	1607:1626	the sublingual route	1607:1626	The polymeric scaffold with a high safety and efficacy holds a new promising delivery strategy for administering injectable insulin through the sublingual route.					
35151706	6	45	theme	blood	1053:1057	arg1	level					1067:1071	blood glucose level	1053:1071	blood glucose level	1053:1071	Pharmacokinetic parameters and blood glucose level were evaluated following sublingual administration of scaffolds to type-1 diabetic rats.					
35151706	6	46	theme	sublingual	1098:1107	arg1	administration					1109:1122	sublingual administration	1098:1122	sublingual administration of scaffolds to type-1 diabetic rats	1098:1159	Pharmacokinetic parameters and blood glucose level were evaluated following sublingual administration of scaffolds to type-1 diabetic rats.					
35151706	2	47	theme	thermal	544:550	arg1	features					584:591	the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features	503:591	the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features	503:591	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	7	48	theme	scaffold	1179:1186	arg1	dose					1171:1174	A single dose	1162:1174	A single dose of scaffold	1162:1186	A single dose of scaffold presented a longer hypoglycemic effect, reducing ~60% of glycemia after 30 min and it lasted for 12 h by increasing the bioavailability of insulin.					
35151706	1	49	theme	sublingual	218:227	arg1	administration					229:242	sublingual administration	218:242	sublingual administration	218:242	Delivery of therapeutic peptides via sublingual administration is extremely desired and 3D printed scaffolds are potential candidates as carriers to enhance insulin delivery.					
35151706	2	50	theme	insulin	473:479	arg1	delivery					481:488	insulin delivery	473:488	insulin delivery	473:488	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	8	51	theme	insulin	1386:1392	arg1	levels					1394:1399	serum insulin levels	1380:1399	serum insulin levels	1380:1399	Scaffolds indicated a sustained profile for serum insulin levels, which continued to increase slightly after 3 h during the study.					
35151706	0	52	theme	printed	38:44	arg1	scaffolds					82:90	3D printed sodium alginate/polyethylene glycol scaffolds	35:90	3D printed sodium alginate/polyethylene glycol scaffolds	35:90	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	0	53	dep	evaluation	21:30	arg1	characterization					141:156	characterization	141:156	characterization	141:156	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	0	53	dep	evaluation	21:30	arg1	pharmacokinetics					163:178	pharmacokinetics	163:178	pharmacokinetics	163:178	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	0	53	dep	evaluation	21:30	arg1	Preparation					128:138	Preparation	128:138	Preparation	128:138	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	8	54	theme	serum	1380:1384	arg1	levels					1394:1399	serum insulin levels	1380:1399	serum insulin levels	1380:1399	Scaffolds indicated a sustained profile for serum insulin levels, which continued to increase slightly after 3 h during the study.					
35151706	2	55	theme	printed	359:365	arg1	PEG					420:422	PEG	420:422	PEG	420:422	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	2	55	theme	printed	359:365	arg1	glycol					412:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol	356:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds	356:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	7	56	theme	single	1164:1169	arg1	dose					1171:1174	A single dose	1162:1174	A single dose of scaffold	1162:1186	A single dose of scaffold presented a longer hypoglycemic effect, reducing ~60% of glycemia after 30 min and it lasted for 12 h by increasing the bioavailability of insulin.					
35151706	3	57	theme	loading	660:666	arg1	insulin					668:674	loading insulin	660:674	loading insulin	660:674	The tensile strength and flexibility of scaffolds increased after loading insulin due to the crystalline structure of insulin.					
35151706	3	58	theme	tensile	598:604	arg1	strength					606:613	tensile strength	598:613	tensile strength	598:613	The tensile strength and flexibility of scaffolds increased after loading insulin due to the crystalline structure of insulin.					
35151706	2	59	theme	3D	356:357	arg1	PEG					420:422	PEG	420:422	PEG	420:422	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	2	59	theme	3D	356:357	arg1	glycol					412:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol	356:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds	356:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	1	60	theme	insulin	338:344	arg1	delivery					346:353	insulin delivery	338:353	insulin delivery	338:353	Delivery of therapeutic peptides via sublingual administration is extremely desired and 3D printed scaffolds are potential candidates as carriers to enhance insulin delivery.					
35151706	2	61	theme	pharmacokinetic	568:582	arg1	features					584:591	the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features	503:591	the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features	503:591	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	3	62	theme	scaffolds	634:642	arg1	strength					606:613	tensile strength	598:613	tensile strength	598:613	The tensile strength and flexibility of scaffolds increased after loading insulin due to the crystalline structure of insulin.					
35151706	3	62	theme	scaffolds	634:642	arg1	flexibility					619:629	flexibility	619:629	flexibility	619:629	The tensile strength and flexibility of scaffolds increased after loading insulin due to the crystalline structure of insulin.					
35151706	0	63	dep	in	13:14	arg1	vivo					16:19	vivo	16:19	vivo	16:19	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	6	64	theme	diabetic	1147:1154	arg1	rats					1156:1159	type-1 diabetic rats	1140:1159	type-1 diabetic rats	1140:1159	Pharmacokinetic parameters and blood glucose level were evaluated following sublingual administration of scaffolds to type-1 diabetic rats.					
35151706	2	65	theme	morphological	517:529	arg1	features					584:591	the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features	503:591	the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features	503:591	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	5	66	theme	L929	918:921	arg1	cells					923:927	L929 cells	918:927	L929 cells	918:927	The cell viability of L929 cells on 3D printed scaffolds was examined and these scaffolds could be safely applied on animals.					
35151706	8	67	theme	sustained	1358:1366	arg1	profile					1368:1374	a sustained profile	1356:1374	a sustained profile for serum insulin levels, which continued to increase slightly after 3 h during the study	1356:1464	Scaffolds indicated a sustained profile for serum insulin levels, which continued to increase slightly after 3 h during the study.					
35151706	2	68	theme	chemical	507:514	arg1	features					584:591	the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features	503:591	the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features	503:591	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	6	69	theme	Pharmacokinetic	1022:1036	arg1	parameters					1038:1047	Pharmacokinetic parameters	1022:1047	Pharmacokinetic parameters	1022:1047	Pharmacokinetic parameters and blood glucose level were evaluated following sublingual administration of scaffolds to type-1 diabetic rats.					
35151706	0	70	theme	sodium	46:51	arg1	scaffolds					82:90	3D printed sodium alginate/polyethylene glycol scaffolds	35:90	3D printed sodium alginate/polyethylene glycol scaffolds	35:90	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	6	71	theme	scaffolds	1127:1135	arg1	administration					1109:1122	sublingual administration	1098:1122	sublingual administration of scaffolds to type-1 diabetic rats	1098:1159	Pharmacokinetic parameters and blood glucose level were evaluated following sublingual administration of scaffolds to type-1 diabetic rats.					
35151706	2	72	theme	composite	425:433	arg1	scaffolds					435:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds	356:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds	356:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	7	73	theme	glycemia	1245:1252	arg1	%					1240:1240	~60%	1237:1240	~60% of glycemia	1237:1252	A single dose of scaffold presented a longer hypoglycemic effect, reducing ~60% of glycemia after 30 min and it lasted for 12 h by increasing the bioavailability of insulin.					
35151706	7	73	theme	glycemia	1245:1252	arg1	glycemia					1245:1252	glycemia	1245:1252	glycemia	1245:1252	A single dose of scaffold presented a longer hypoglycemic effect, reducing ~60% of glycemia after 30 min and it lasted for 12 h by increasing the bioavailability of insulin.					
35151706	0	74	theme	3D	35:36	arg1	scaffolds					82:90	3D printed sodium alginate/polyethylene glycol scaffolds	35:90	3D printed sodium alginate/polyethylene glycol scaffolds	35:90	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	0	75	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro and in vivo evaluation of 3D printed sodium alginate/polyethylene glycol scaffolds for sublingual delivery of insulin: Preparation, characterization, and pharmacokinetics.					
35151706	2	76	theme	alginate	385:392	arg1	PEG					420:422	PEG	420:422	PEG	420:422	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	2	76	theme	alginate	385:392	arg1	glycol					412:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol	356:417	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds	356:443	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
35151706	2	77	theme	mechanical	532:541	arg1	features					584:591	the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features	503:591	the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features	503:591	3D printed sublingual sodium alginate (SA)/polyethylene glycol (PEG) composite scaffolds were produced for enhancing insulin delivery by examining the chemical, morphological, mechanical, thermal, cytotoxic, and pharmacokinetic features.					
36610565	11	0	theme	excellent	1294:1302	arg1	selectivity					1315:1325	excellent adsorption selectivity	1294:1325	excellent adsorption selectivity	1294:1325	SiO2-FP displays excellent adsorption selectivity and can 100 % adsorb Hg(II) with the coexisting of Ni(II), Zn(II), Pb(II), Mn(II), and Co(II).					
36610565	11	1	theme	%	1339:1339	arg1	II					1351:1352	II	1351:1352	II	1351:1352	SiO2-FP displays excellent adsorption selectivity and can 100 % adsorb Hg(II) with the coexisting of Ni(II), Zn(II), Pb(II), Mn(II), and Co(II).					
36610565	11	1	theme	%	1339:1339	arg1	Hg					1348:1349	% adsorb Hg	1339:1349	% adsorb Hg(II) with the coexisting of Ni(II), Zn(II), Pb(II), Mn(II), and Co(II)	1339:1419	SiO2-FP displays excellent adsorption selectivity and can 100 % adsorb Hg(II) with the coexisting of Ni(II), Zn(II), Pb(II), Mn(II), and Co(II).					
36610565	11	2	theme	adsorption	1304:1313	arg1	selectivity					1315:1325	excellent adsorption selectivity	1294:1325	excellent adsorption selectivity	1294:1325	SiO2-FP displays excellent adsorption selectivity and can 100 % adsorb Hg(II) with the coexisting of Ni(II), Zn(II), Pb(II), Mn(II), and Co(II).					
36610565	0	3	theme	Hg	101:102	arg1	adsorption					87:96	efficient adsorption	77:96	efficient adsorption of Hg(II)	77:106	Synthesis of hyperbranched polyamine dendrimer/chitosan/silica composite for efficient adsorption of Hg(II).					
36610565	6	4	theme	optimal	744:750	arg1	6					796:796	6	796:796	6	796:796	The optimal solution pH for the adsorption of Hg(II) is 6.					
36610565	6	4	theme	optimal	744:750	arg1	pH					761:762	The optimal solution pH	740:762	The optimal solution pH for the adsorption of Hg(II)	740:791	The optimal solution pH for the adsorption of Hg(II) is 6.					
36610565	7	5	theme	kinetic	810:816	arg1	Adsorption					799:808	Adsorption kinetic	799:816	Adsorption kinetic	799:816	Adsorption kinetic indicates the adsorption for Hg(II) can reach equilibrium at 250 min.					
36610565	10	6	theme	adsorption	1109:1118	arg1	isotherm					1120:1127	The adsorption isotherm	1105:1127	The adsorption isotherm	1105:1127	The adsorption isotherm indicates the adsorption process can be described by Langmuir model and the adsorption is a spontaneous, endothermic and entropy-increased process.					
36610565	3	7	theme	hyperbranched	307:319	arg1	SiO2-FP					368:374	SiO2-FP	368:374	SiO2-FP	368:374	Herein, hyperbranched polyamine dendrimer/chitosan/silica composite (SiO2-FP) was synthesized for the adsorption of aqueous Hg(II).					
36610565	3	7	theme	hyperbranched	307:319	arg1	composite					357:365	hyperbranched polyamine dendrimer/chitosan/silica composite	307:365	hyperbranched polyamine dendrimer/chitosan/silica composite (SiO2-FP)	307:375	Herein, hyperbranched polyamine dendrimer/chitosan/silica composite (SiO2-FP) was synthesized for the adsorption of aqueous Hg(II).					
36610565	6	8	theme	solution	752:759	arg1	6					796:796	6	796:796	6	796:796	The optimal solution pH for the adsorption of Hg(II) is 6.					
36610565	6	8	theme	solution	752:759	arg1	pH					761:762	The optimal solution pH	740:762	The optimal solution pH for the adsorption of Hg(II)	740:791	The optimal solution pH for the adsorption of Hg(II) is 6.					
36610565	5	9	theme	0.79 mmol·g-1	632:644	arg1	capacity					620:627	the adsorption capacity	605:627	the adsorption capacity of 0.79 mmol·g-1, which is higher than the corresponding chitosan functionalized silica (SiO2-CTS) by 46.30 %	605:737	SiO2-FP displays good adsorption performance for Hg(II) with the adsorption capacity of 0.79 mmol·g-1, which is higher than the corresponding chitosan functionalized silica (SiO2-CTS) by 46.30 %.					
36610565	8	10	theme	kinetic	899:905	arg1	process					907:913	Adsorption kinetic process	888:913	Adsorption kinetic process	888:913	Adsorption kinetic process can be well fitted by pseudo-second-order (PSO).					
36610565	3	11	theme	polyamine	321:329	arg1	SiO2-FP					368:374	SiO2-FP	368:374	SiO2-FP	368:374	Herein, hyperbranched polyamine dendrimer/chitosan/silica composite (SiO2-FP) was synthesized for the adsorption of aqueous Hg(II).					
36610565	3	11	theme	polyamine	321:329	arg1	composite					357:365	hyperbranched polyamine dendrimer/chitosan/silica composite	307:365	hyperbranched polyamine dendrimer/chitosan/silica composite (SiO2-FP)	307:375	Herein, hyperbranched polyamine dendrimer/chitosan/silica composite (SiO2-FP) was synthesized for the adsorption of aqueous Hg(II).					
36610565	9	12	theme	adsorption	1081:1090	arg1	temperature					1092:1102	adsorption temperature	1081:1102	adsorption temperature	1081:1102	Adsorption isotherm reveals the adsorption for Hg(II) can be promoted by increasing initial Hg(II) concentration and adsorption temperature.					
36610565	11	13	theme	Ni	1378:1379	arg1	coexisting					1364:1373	coexisting	1364:1373	coexisting	1364:1373	SiO2-FP displays excellent adsorption selectivity and can 100 % adsorb Hg(II) with the coexisting of Ni(II), Zn(II), Pb(II), Mn(II), and Co(II).					
36610565	2	14	theme	promising	240:248	arg1	Adsorption					207:216	Adsorption	207:216	Adsorption	207:216	Adsorption is considered to be a promising strategy to remove Hg(II) from aqueous solution.					
36610565	2	14	theme	promising	240:248	arg1	strategy					250:257	a promising strategy	238:257	a promising strategy to remove Hg(II) from aqueous solution	238:296	Adsorption is considered to be a promising strategy to remove Hg(II) from aqueous solution.					
36610565	13	15	theme	adsorption-desorption	1635:1655	arg1	cycles					1657:1662	five adsorption-desorption cycles	1630:1662	five adsorption-desorption cycles	1630:1662	SiO2-FP exhibits good regeneration property and the regeneration rate can maintain approximately 90 % after five adsorption-desorption cycles.					
36610565	3	16	theme	aqueous	415:421	arg1	II					426:427	II	426:427	II	426:427	Herein, hyperbranched polyamine dendrimer/chitosan/silica composite (SiO2-FP) was synthesized for the adsorption of aqueous Hg(II).					
36610565	3	16	theme	aqueous	415:421	arg1	Hg					423:424	aqueous Hg	415:424	aqueous Hg(II)	415:428	Herein, hyperbranched polyamine dendrimer/chitosan/silica composite (SiO2-FP) was synthesized for the adsorption of aqueous Hg(II).					
36610565	4	17	theme	adsorption	435:444	arg1	performance					446:456	The adsorption performance	431:456	The adsorption performance of SiO2-FP	431:467	The adsorption performance of SiO2-FP was comprehensively determined by considering various influencing factors.					
36610565	1	18	with	pollution	113:121	arg1	Hg					144:145	Hg	144:145	Hg(II)	144:149	The pollution of water system with Hg(II) exerts hazardous effect to ecosystem and public health.					
36610565	1	18	with	pollution	113:121	arg1	II					147:148	II	147:148	II	147:148	The pollution of water system with Hg(II) exerts hazardous effect to ecosystem and public health.					
36610565	5	19	theme	good	561:564	arg1	performance					577:587	good adsorption performance	561:587	good adsorption performance for Hg(II)	561:598	SiO2-FP displays good adsorption performance for Hg(II) with the adsorption capacity of 0.79 mmol·g-1, which is higher than the corresponding chitosan functionalized silica (SiO2-CTS) by 46.30 %.					
36610565	10	20	theme	Langmuir	1182:1189	arg1	model					1191:1195	Langmuir model	1182:1195	Langmuir model	1182:1195	The adsorption isotherm indicates the adsorption process can be described by Langmuir model and the adsorption is a spontaneous, endothermic and entropy-increased process.					
36610565	1	21	theme	hazardous	158:166	arg1	effect					168:173	hazardous effect	158:173	hazardous effect	158:173	The pollution of water system with Hg(II) exerts hazardous effect to ecosystem and public health.					
36610565	5	22	theme	adsorption	609:618	arg1	capacity					620:627	the adsorption capacity	605:627	the adsorption capacity of 0.79 mmol·g-1, which is higher than the corresponding chitosan functionalized silica (SiO2-CTS) by 46.30 %	605:737	SiO2-FP displays good adsorption performance for Hg(II) with the adsorption capacity of 0.79 mmol·g-1, which is higher than the corresponding chitosan functionalized silica (SiO2-CTS) by 46.30 %.					
36610565	5	23	theme	adsorption	566:575	arg1	performance					577:587	good adsorption performance	561:587	good adsorption performance for Hg(II)	561:598	SiO2-FP displays good adsorption performance for Hg(II) with the adsorption capacity of 0.79 mmol·g-1, which is higher than the corresponding chitosan functionalized silica (SiO2-CTS) by 46.30 %.					
36610565	10	24	theme	spontaneous	1221:1231	arg1	adsorption					1205:1214	the adsorption	1201:1214	the adsorption	1201:1214	The adsorption isotherm indicates the adsorption process can be described by Langmuir model and the adsorption is a spontaneous, endothermic and entropy-increased process.					
36610565	10	24	theme	spontaneous	1221:1231	arg1	process					1268:1274	a spontaneous, endothermic and entropy-increased process	1219:1274	a spontaneous, endothermic and entropy-increased process	1219:1274	The adsorption isotherm indicates the adsorption process can be described by Langmuir model and the adsorption is a spontaneous, endothermic and entropy-increased process.					
36610565	3	25	theme	dendrimer/chitosan/silica	331:355	arg1	SiO2-FP					368:374	SiO2-FP	368:374	SiO2-FP	368:374	Herein, hyperbranched polyamine dendrimer/chitosan/silica composite (SiO2-FP) was synthesized for the adsorption of aqueous Hg(II).					
36610565	3	25	theme	dendrimer/chitosan/silica	331:355	arg1	composite					357:365	hyperbranched polyamine dendrimer/chitosan/silica composite	307:365	hyperbranched polyamine dendrimer/chitosan/silica composite (SiO2-FP)	307:375	Herein, hyperbranched polyamine dendrimer/chitosan/silica composite (SiO2-FP) was synthesized for the adsorption of aqueous Hg(II).					
36610565	4	26	theme	influencing	523:533	arg1	factors					535:541	various influencing factors	515:541	various influencing factors	515:541	The adsorption performance of SiO2-FP was comprehensively determined by considering various influencing factors.					
36610565	0	27	theme	hyperbranched	13:25	arg1	composite					63:71	hyperbranched polyamine dendrimer/chitosan/silica composite	13:71	hyperbranched polyamine dendrimer/chitosan/silica composite	13:71	Synthesis of hyperbranched polyamine dendrimer/chitosan/silica composite for efficient adsorption of Hg(II).					
36610565	5	28	theme	corresponding	672:684	arg1	chitosan					686:693	the corresponding chitosan	668:693	the corresponding chitosan functionalized silica (SiO2-CTS) by 46.30 %	668:737	SiO2-FP displays good adsorption performance for Hg(II) with the adsorption capacity of 0.79 mmol·g-1, which is higher than the corresponding chitosan functionalized silica (SiO2-CTS) by 46.30 %.					
36610565	0	29	theme	composite	63:71	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of hyperbranched polyamine dendrimer/chitosan/silica composite for efficient adsorption of Hg(II).	0:107	Synthesis of hyperbranched polyamine dendrimer/chitosan/silica composite for efficient adsorption of Hg(II).					
36610565	12	30	theme	Adsorption	1422:1431	arg1	mechanism					1433:1441	Adsorption mechanism	1422:1441	Adsorption mechanism	1422:1441	Adsorption mechanism demonstrates -NH-, -NH2, CN, CONH, -OH, and CO participated in the adsorption.					
36610565	3	31	theme	Hg	423:424	arg1	adsorption					401:410	the adsorption	397:410	the adsorption of aqueous Hg(II)	397:428	Herein, hyperbranched polyamine dendrimer/chitosan/silica composite (SiO2-FP) was synthesized for the adsorption of aqueous Hg(II).					
36610565	0	32	theme	dendrimer/chitosan/silica	37:61	arg1	composite					63:71	hyperbranched polyamine dendrimer/chitosan/silica composite	13:71	hyperbranched polyamine dendrimer/chitosan/silica composite	13:71	Synthesis of hyperbranched polyamine dendrimer/chitosan/silica composite for efficient adsorption of Hg(II).					
36610565	0	33	theme	polyamine	27:35	arg1	composite					63:71	hyperbranched polyamine dendrimer/chitosan/silica composite	13:71	hyperbranched polyamine dendrimer/chitosan/silica composite	13:71	Synthesis of hyperbranched polyamine dendrimer/chitosan/silica composite for efficient adsorption of Hg(II).					
36610565	10	34	theme	entropy-increased	1250:1266	arg1	adsorption					1205:1214	the adsorption	1201:1214	the adsorption	1201:1214	The adsorption isotherm indicates the adsorption process can be described by Langmuir model and the adsorption is a spontaneous, endothermic and entropy-increased process.					
36610565	10	34	theme	entropy-increased	1250:1266	arg1	process					1268:1274	a spontaneous, endothermic and entropy-increased process	1219:1274	a spontaneous, endothermic and entropy-increased process	1219:1274	The adsorption isotherm indicates the adsorption process can be described by Langmuir model and the adsorption is a spontaneous, endothermic and entropy-increased process.					
36610565	1	35	theme	public	192:197	arg1	health					199:204	public health	192:204	public health	192:204	The pollution of water system with Hg(II) exerts hazardous effect to ecosystem and public health.					
36610565	0	36	theme	efficient	77:85	arg1	adsorption					87:96	efficient adsorption	77:96	efficient adsorption of Hg(II)	77:106	Synthesis of hyperbranched polyamine dendrimer/chitosan/silica composite for efficient adsorption of Hg(II).					
36610565	2	37	attach	remove	262:267	arg1	solution					289:296	aqueous solution	281:296	aqueous solution	281:296	Adsorption is considered to be a promising strategy to remove Hg(II) from aqueous solution.					
36610565	2	37	attach	remove	262:267	arg2	strategy					250:257	a promising strategy	238:257	a promising strategy to remove Hg(II) from aqueous solution	238:296	Adsorption is considered to be a promising strategy to remove Hg(II) from aqueous solution.					
36610565	2	37	attach	remove	262:267	arg2	Adsorption					207:216	Adsorption	207:216	Adsorption	207:216	Adsorption is considered to be a promising strategy to remove Hg(II) from aqueous solution.					
36610565	4	38	theme	various	515:521	arg1	factors					535:541	various influencing factors	515:541	various influencing factors	515:541	The adsorption performance of SiO2-FP was comprehensively determined by considering various influencing factors.					
36610565	11	39	with	Hg	1348:1349	arg1	coexisting					1364:1373	coexisting	1364:1373	coexisting	1364:1373	SiO2-FP displays excellent adsorption selectivity and can 100 % adsorb Hg(II) with the coexisting of Ni(II), Zn(II), Pb(II), Mn(II), and Co(II).					
36610565	10	40	theme	endothermic	1234:1244	arg1	adsorption					1205:1214	the adsorption	1201:1214	the adsorption	1201:1214	The adsorption isotherm indicates the adsorption process can be described by Langmuir model and the adsorption is a spontaneous, endothermic and entropy-increased process.					
36610565	10	40	theme	endothermic	1234:1244	arg1	process					1268:1274	a spontaneous, endothermic and entropy-increased process	1219:1274	a spontaneous, endothermic and entropy-increased process	1219:1274	The adsorption isotherm indicates the adsorption process can be described by Langmuir model and the adsorption is a spontaneous, endothermic and entropy-increased process.					
36610565	13	41	theme	good	1539:1542	arg1	property					1557:1564	good regeneration property	1539:1564	good regeneration property	1539:1564	SiO2-FP exhibits good regeneration property and the regeneration rate can maintain approximately 90 % after five adsorption-desorption cycles.					
36610565	5	42	dep	silica	710:715	arg1	%					737:737	46.30 %	731:737	46.30 %	731:737	SiO2-FP displays good adsorption performance for Hg(II) with the adsorption capacity of 0.79 mmol·g-1, which is higher than the corresponding chitosan functionalized silica (SiO2-CTS) by 46.30 %.					
36610565	9	43	theme	initial	1048:1054	arg1	concentration					1063:1075	initial Hg(II) concentration	1048:1075	initial Hg(II) concentration	1048:1075	Adsorption isotherm reveals the adsorption for Hg(II) can be promoted by increasing initial Hg(II) concentration and adsorption temperature.					
36610565	1	44	theme	water	126:130	arg1	system					132:137	water system	126:137	water system	126:137	The pollution of water system with Hg(II) exerts hazardous effect to ecosystem and public health.					
36610565	10	45	theme	adsorption	1143:1152	arg1	process					1154:1160	the adsorption process	1139:1160	the adsorption process	1139:1160	The adsorption isotherm indicates the adsorption process can be described by Langmuir model and the adsorption is a spontaneous, endothermic and entropy-increased process.					
36610565	11	46	dep	Hg	1348:1349	arg1	adsorb					1341:1346	adsorb	1341:1346	adsorb	1341:1346	SiO2-FP displays excellent adsorption selectivity and can 100 % adsorb Hg(II) with the coexisting of Ni(II), Zn(II), Pb(II), Mn(II), and Co(II).					
36610565	9	47	theme	Hg	1056:1057	arg1	concentration					1063:1075	initial Hg(II) concentration	1048:1075	initial Hg(II) concentration	1048:1075	Adsorption isotherm reveals the adsorption for Hg(II) can be promoted by increasing initial Hg(II) concentration and adsorption temperature.					
36610565	1	48	theme	system	132:137	arg1	pollution					113:121	The pollution	109:121	The pollution of water system with Hg(II)	109:149	The pollution of water system with Hg(II) exerts hazardous effect to ecosystem and public health.					
36610565	9	49	dep	concentration	1063:1075	arg1	II					1059:1060	II	1059:1060	II	1059:1060	Adsorption isotherm reveals the adsorption for Hg(II) can be promoted by increasing initial Hg(II) concentration and adsorption temperature.					
36610565	2	50	theme	aqueous	281:287	arg1	solution					289:296	aqueous solution	281:296	aqueous solution	281:296	Adsorption is considered to be a promising strategy to remove Hg(II) from aqueous solution.					
36610565	6	51	theme	Hg	786:787	arg1	adsorption					772:781	the adsorption	768:781	the adsorption of Hg(II)	768:791	The optimal solution pH for the adsorption of Hg(II) is 6.					
36610565	13	52	theme	regeneration	1574:1585	arg1	rate					1587:1590	the regeneration rate	1570:1590	the regeneration rate	1570:1590	SiO2-FP exhibits good regeneration property and the regeneration rate can maintain approximately 90 % after five adsorption-desorption cycles.					
36610565	4	53	theme	SiO2-FP	461:467	arg1	performance					446:456	The adsorption performance	431:456	The adsorption performance of SiO2-FP	431:467	The adsorption performance of SiO2-FP was comprehensively determined by considering various influencing factors.					
36610565	8	54	theme	Adsorption	888:897	arg1	process					907:913	Adsorption kinetic process	888:913	Adsorption kinetic process	888:913	Adsorption kinetic process can be well fitted by pseudo-second-order (PSO).					
36610565	9	55	theme	Adsorption	964:973	arg1	isotherm					975:982	Adsorption isotherm	964:982	Adsorption isotherm	964:982	Adsorption isotherm reveals the adsorption for Hg(II) can be promoted by increasing initial Hg(II) concentration and adsorption temperature.					
36610565	13	56	theme	regeneration	1544:1555	arg1	property					1557:1564	good regeneration property	1539:1564	good regeneration property	1539:1564	SiO2-FP exhibits good regeneration property and the regeneration rate can maintain approximately 90 % after five adsorption-desorption cycles.					
36152699	2	0	theme	pH	426:427	arg1	value					429:433	the pH value	422:433	the pH value	422:433	The turbidity results showed that the composite gel had the maximum polymer concentration when the mixing ratio was 2:1 and the pH value was 3.35.					
36152699	2	0	theme	pH	426:427	arg1	3.35					439:442	3.35	439:442	3.35	439:442	The turbidity results showed that the composite gel had the maximum polymer concentration when the mixing ratio was 2:1 and the pH value was 3.35.					
36152699	3	1	theme	hydrogel	616:623	arg1	properties					598:607	the properties	594:607	the properties of the hydrogel at this Ca2+ concentration	594:650	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	6	2	theme	delivery	1199:1206	arg1	system					1208:1213	a promising delivery system	1187:1213	a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components	1187:1343	To sum up, THIH was a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components.					
36152699	6	2	theme	delivery	1199:1206	arg1	THIH					1178:1181	THIH	1178:1181	THIH	1178:1181	To sum up, THIH was a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components.					
36152699	5	3	dep	in	1071:1072	arg1	vitro					1074:1078	vitro	1074:1078	vitro	1074:1078	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	2	4	theme	turbidity	302:310	arg1	results					312:318	The turbidity results	298:318	The turbidity results	298:318	The turbidity results showed that the composite gel had the maximum polymer concentration when the mixing ratio was 2:1 and the pH value was 3.35.					
36152699	0	5	theme	Tenebrio	71:78	arg1	Molitor					80:86	Tenebrio Molitor	71:86	Tenebrio Molitor	71:86	Encapsulation and sustained release of curcumin by hawthorn pectin and Tenebrio Molitor protein composite hydrogel.					
36152699	5	6	theme	gastrointestinal	1041:1056	arg1	environment					1058:1068	simulated gastrointestinal environment	1031:1068	simulated gastrointestinal environment	1031:1068	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	5	7	theme	storage	961:967	arg1	stability					969:977	storage stability	961:977	storage stability	961:977	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	1	8	theme	pH	146:147	arg1	value					149:153	pH value	146:153	pH value	146:153	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	1	9	theme	hawthorn	223:230	arg1	HP					240:241	HP	240:241	HP	240:241	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	1	9	theme	hawthorn	223:230	arg1	pectin					232:237	hawthorn pectin	223:237	hawthorn pectin (HP)	223:242	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	0	10	theme	composite	96:104	arg1	hydrogel					106:113	protein composite hydrogel	88:113	protein composite hydrogel	88:113	Encapsulation and sustained release of curcumin by hawthorn pectin and Tenebrio Molitor protein composite hydrogel.					
36152699	4	11	theme	scanning	700:707	arg1	microscope					718:727	scanning electron microscope	700:727	scanning electron microscope	700:727	The results of scanning electron microscope and pore size also proved that the network structure prepared under this condition was compact and uniform, the pore size was small, which was beneficial to the entrapment of active components.					
36152699	6	12	theme	active	1327:1332	arg1	components					1334:1343	active components	1327:1343	active components	1327:1343	To sum up, THIH was a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components.					
36152699	1	13	theme	pectin	232:237	arg1	gelation					211:218	the complex gelation	199:218	the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP)	199:277	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	0	14	theme	protein	88:94	arg1	hydrogel					106:113	protein composite hydrogel	88:113	protein composite hydrogel	88:113	Encapsulation and sustained release of curcumin by hawthorn pectin and Tenebrio Molitor protein composite hydrogel.					
36152699	3	15	theme	Ca2+	633:636	arg1	concentration					638:650	this Ca2+ concentration	628:650	this Ca2+ concentration	628:650	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	6	16	dep	application	1226:1236	arg1	prospects					1238:1246	prospects	1238:1246	prospects	1238:1246	To sum up, THIH was a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components.					
36152699	1	17	from	effects	135:141	arg1	gelation					211:218	the complex gelation	199:218	the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP)	199:277	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	3	18	theme	measurement	461:471	arg1	results					473:479	The rheological measurement results	445:479	The rheological measurement results	445:479	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	1	19	theme	value	149:153	arg1	effects					135:141	the effects	131:141	the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP)	131:277	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	5	20	from	stability	969:977	arg1	environment					1058:1068	simulated gastrointestinal environment	1031:1068	simulated gastrointestinal environment	1031:1068	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	5	21	theme	THIH-loaded	1007:1017	arg1	curcumin					1019:1026	THIH-loaded curcumin	1007:1026	THIH-loaded curcumin	1007:1026	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	3	22	from	properties	598:607	arg1	concentration					638:650	this Ca2+ concentration	628:650	this Ca2+ concentration	628:650	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	2	23	theme	composite	336:344	arg1	gel					346:348	the composite gel	332:348	the composite gel	332:348	The turbidity results showed that the composite gel had the maximum polymer concentration when the mixing ratio was 2:1 and the pH value was 3.35.					
36152699	2	24	theme	mixing	397:402	arg1	ratio					404:408	the mixing ratio	393:408	the mixing ratio	393:408	The turbidity results showed that the composite gel had the maximum polymer concentration when the mixing ratio was 2:1 and the pH value was 3.35.					
36152699	5	25	theme	curcumin	1019:1026	arg1	stability					969:977	storage stability	961:977	storage stability	961:977	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	5	25	theme	curcumin	1019:1026	arg1	activity					995:1002	antioxidant activity	983:1002	antioxidant activity	983:1002	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	3	26	theme	15 mmol/L	501:509	arg1	THIH					522:525	THIH	522:525	THIH	522:525	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	3	26	theme	15 mmol/L	501:509	arg1	hydrogel					512:519	TMP/HP (15 mmol/L) hydrogel	493:519	TMP/HP (15 mmol/L) hydrogel (THIH)	493:526	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	5	27	theme	simulated	1080:1088	arg1	experiment					1100:1109	in vitro simulated digestion experiment	1071:1109	in vitro simulated digestion experiment	1071:1109	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	1	28	theme	mixing	156:161	arg1	ratio					163:167	mixing ratio	156:167	mixing ratio	156:167	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	5	29	theme	digestion	1090:1098	arg1	experiment					1100:1109	in vitro simulated digestion experiment	1071:1109	in vitro simulated digestion experiment	1071:1109	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	4	30	theme	network	764:770	arg1	structure					772:780	the network structure	760:780	the network structure prepared under this condition	760:810	The results of scanning electron microscope and pore size also proved that the network structure prepared under this condition was compact and uniform, the pore size was small, which was beneficial to the entrapment of active components.					
36152699	4	30	theme	network	764:770	arg1	compact					816:822	compact	816:822	compact	816:822	The results of scanning electron microscope and pore size also proved that the network structure prepared under this condition was compact and uniform, the pore size was small, which was beneficial to the entrapment of active components.					
36152699	1	31	theme	ratio	163:167	arg1	effects					135:141	the effects	131:141	the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP)	131:277	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	5	32	from	activity	995:1002	arg1	environment					1058:1068	simulated gastrointestinal environment	1031:1068	simulated gastrointestinal environment	1031:1068	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	3	33	theme	TMP/HP	493:498	arg1	THIH					522:525	THIH	522:525	THIH	522:525	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	3	33	theme	TMP/HP	493:498	arg1	hydrogel					512:519	TMP/HP (15 mmol/L) hydrogel	493:519	TMP/HP (15 mmol/L) hydrogel (THIH)	493:526	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	6	34	with	system	1208:1213	arg1	application					1226:1236	broad application prospects	1220:1246	broad application prospects	1220:1246	To sum up, THIH was a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components.					
36152699	2	35	theme	polymer	366:372	arg1	concentration					374:386	the maximum polymer concentration	354:386	the maximum polymer concentration	354:386	The turbidity results showed that the composite gel had the maximum polymer concentration when the mixing ratio was 2:1 and the pH value was 3.35.					
36152699	0	36	theme	sustained	18:26	arg1	release					28:34	sustained release	18:34	sustained release	18:34	Encapsulation and sustained release of curcumin by hawthorn pectin and Tenebrio Molitor protein composite hydrogel.					
36152699	5	37	theme	simulated	1031:1039	arg1	environment					1058:1068	simulated gastrointestinal environment	1031:1068	simulated gastrointestinal environment	1031:1068	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	6	38	dep	entrapment	1300:1309	arg1	the					1296:1298	the	1296:1298	the	1296:1298	To sum up, THIH was a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components.					
36152699	3	39	theme	loss	564:567	arg1	modulus					569:575	loss modulus	564:575	loss modulus	564:575	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	0	40	dep	pectin	60:65	arg1	hydrogel					106:113	protein composite hydrogel	88:113	protein composite hydrogel	88:113	Encapsulation and sustained release of curcumin by hawthorn pectin and Tenebrio Molitor protein composite hydrogel.					
36152699	2	41	theme	maximum	358:364	arg1	concentration					374:386	the maximum polymer concentration	354:386	the maximum polymer concentration	354:386	The turbidity results showed that the composite gel had the maximum polymer concentration when the mixing ratio was 2:1 and the pH value was 3.35.					
36152699	0	42	theme	curcumin	39:46	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation	0:12	Encapsulation and sustained release of curcumin by hawthorn pectin and Tenebrio Molitor protein composite hydrogel.					
36152699	0	42	theme	curcumin	39:46	arg1	release					28:34	sustained release	18:34	sustained release	18:34	Encapsulation and sustained release of curcumin by hawthorn pectin and Tenebrio Molitor protein composite hydrogel.					
36152699	1	43	theme	Tenebrio	248:255	arg1	Molitor					257:263	Tenebrio Molitor	248:263	Tenebrio Molitor protein (TMP)	248:277	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	4	44	theme	size	738:741	arg1	results					689:695	The results	685:695	The results of scanning electron microscope and pore size	685:741	The results of scanning electron microscope and pore size also proved that the network structure prepared under this condition was compact and uniform, the pore size was small, which was beneficial to the entrapment of active components.					
36152699	1	45	theme	Ca2+	177:180	arg1	concentration					182:194	the Ca2+ concentration	173:194	the Ca2+ concentration	173:194	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	1	46	theme	Molitor	257:263	arg1	protein					265:271	Tenebrio Molitor protein	248:271	Tenebrio Molitor protein (TMP)	248:277	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	1	46	theme	Molitor	257:263	arg1	TMP					274:276	TMP	274:276	TMP	274:276	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	4	47	theme	pore	841:844	arg1	small					855:859	small	855:859	small	855:859	The results of scanning electron microscope and pore size also proved that the network structure prepared under this condition was compact and uniform, the pore size was small, which was beneficial to the entrapment of active components.					
36152699	4	47	theme	pore	841:844	arg1	size					846:849	the pore size	837:849	the pore size	837:849	The results of scanning electron microscope and pore size also proved that the network structure prepared under this condition was compact and uniform, the pore size was small, which was beneficial to the entrapment of active components.					
36152699	4	48	theme	pore	733:736	arg1	size					738:741	pore size	733:741	pore size	733:741	The results of scanning electron microscope and pore size also proved that the network structure prepared under this condition was compact and uniform, the pore size was small, which was beneficial to the entrapment of active components.					
36152699	1	49	theme	concentration	182:194	arg1	effects					135:141	the effects	131:141	the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP)	131:277	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	0	50	theme	hawthorn	51:58	arg1	pectin					60:65	hawthorn pectin	51:65	hawthorn pectin	51:65	Encapsulation and sustained release of curcumin by hawthorn pectin and Tenebrio Molitor protein composite hydrogel.					
36152699	1	51	theme	protein	265:271	arg1	gelation					211:218	the complex gelation	199:218	the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP)	199:277	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	5	52	theme	antioxidant	983:993	arg1	activity					995:1002	antioxidant activity	983:1002	antioxidant activity	983:1002	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	5	53	dep	carried	1115:1121	arg1	explore					949:955	explore	949:955	to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment	946:1068	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	4	54	theme	active	904:909	arg1	components					911:920	active components	904:920	active components	904:920	The results of scanning electron microscope and pore size also proved that the network structure prepared under this condition was compact and uniform, the pore size was small, which was beneficial to the entrapment of active components.					
36152699	6	55	theme	broad	1220:1224	arg1	application					1226:1236	broad application prospects	1220:1246	broad application prospects	1220:1246	To sum up, THIH was a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components.					
36152699	2	56	contain	had	350:352	arg2	concentration					374:386	the maximum polymer concentration	354:386	the maximum polymer concentration	354:386	The turbidity results showed that the composite gel had the maximum polymer concentration when the mixing ratio was 2:1 and the pH value was 3.35.					
36152699	2	56	contain	had	350:352	arg1	gel					346:348	the composite gel	332:348	the composite gel	332:348	The turbidity results showed that the composite gel had the maximum polymer concentration when the mixing ratio was 2:1 and the pH value was 3.35.					
36152699	4	57	theme	microscope	718:727	arg1	results					689:695	The results	685:695	The results of scanning electron microscope and pore size	685:741	The results of scanning electron microscope and pore size also proved that the network structure prepared under this condition was compact and uniform, the pore size was small, which was beneficial to the entrapment of active components.					
36152699	6	58	theme	promising	1189:1197	arg1	system					1208:1213	a promising delivery system	1187:1213	a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components	1187:1343	To sum up, THIH was a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components.					
36152699	6	58	theme	promising	1189:1197	arg1	THIH					1178:1181	THIH	1178:1181	THIH	1178:1181	To sum up, THIH was a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components.					
36152699	4	59	theme	electron	709:716	arg1	microscope					718:727	scanning electron microscope	700:727	scanning electron microscope	700:727	The results of scanning electron microscope and pore size also proved that the network structure prepared under this condition was compact and uniform, the pore size was small, which was beneficial to the entrapment of active components.					
36152699	3	60	theme	highest	536:542	arg1	modulus					552:558	storage modulus	544:558	storage modulus	544:558	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	3	61	theme	storage	544:550	arg1	modulus					552:558	storage modulus	544:558	storage modulus	544:558	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	3	62	contain	had	528:530	arg1	THIH					522:525	THIH	522:525	THIH	522:525	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	3	62	contain	had	528:530	arg2	modulus					552:558	storage modulus	544:558	storage modulus	544:558	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	3	62	contain	had	528:530	arg1	hydrogel					512:519	TMP/HP (15 mmol/L) hydrogel	493:519	TMP/HP (15 mmol/L) hydrogel (THIH)	493:526	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	3	62	contain	had	528:530	arg2	modulus					569:575	loss modulus	564:575	loss modulus	564:575	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	6	63	theme	components	1334:1343	arg1	delivery					1315:1322	delivery	1315:1322	delivery	1315:1322	To sum up, THIH was a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components.					
36152699	6	63	theme	components	1334:1343	arg1	entrapment					1300:1309	entrapment	1300:1309	entrapment	1300:1309	To sum up, THIH was a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components.					
36152699	6	64	theme	novel	1281:1285	arg1	idea					1287:1290	a novel idea	1279:1290	a novel idea for the entrapment and delivery of active components	1279:1343	To sum up, THIH was a promising delivery system with broad application prospects, which was expected to provide a novel idea for the entrapment and delivery of active components.					
36152699	1	65	theme	complex	203:209	arg1	gelation					211:218	the complex gelation	199:218	the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP)	199:277	In this study, the effects of pH value, mixing ratio and the Ca2+ concentration on the complex gelation of hawthorn pectin (HP) and Tenebrio Molitor protein (TMP) were investigated.					
36152699	4	66	theme	components	911:920	arg1	entrapment					890:899	the entrapment	886:899	the entrapment of active components	886:920	The results of scanning electron microscope and pore size also proved that the network structure prepared under this condition was compact and uniform, the pore size was small, which was beneficial to the entrapment of active components.					
36152699	3	67	from	concentration	638:650	arg1	properties					598:607	the properties	594:607	the properties of the hydrogel at this Ca2+ concentration	594:650	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	3	67	from	concentration	638:650	arg1	hydrogel					616:623	the hydrogel	612:623	the hydrogel at this Ca2+ concentration	612:650	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	5	68	theme	satisfactory	1131:1142	arg1	results					1144:1150	satisfactory results	1131:1150	satisfactory results	1131:1150	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	3	69	theme	rheological	449:459	arg1	results					473:479	The rheological measurement results	445:479	The rheological measurement results	445:479	The rheological measurement results showed that TMP/HP (15 mmol/L) hydrogel (THIH) had the highest storage modulus and loss modulus, indicating that the properties of the hydrogel at this Ca2+ concentration had been significantly improved.					
36152699	5	70	dep	stability	969:977	arg1	the					957:959	the	957:959	the	957:959	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36152699	5	71	theme	in	1071:1072	arg1	experiment					1100:1109	in vitro simulated digestion experiment	1071:1109	in vitro simulated digestion experiment	1071:1109	Subsequently, in order to explore the storage stability and antioxidant activity of THIH-loaded curcumin in simulated gastrointestinal environment, in vitro simulated digestion experiment was carried out and satisfactory results were obtained.					
36372032	10	0	theme	catalyst	1780:1787	arg1	incorporation					1721:1733	incorporation	1721:1733	incorporation of paper mill sludge and sulfonated graphene catalyst at dosage not exceeding 60 mgSGR/gVS	1721:1824	Finally maximizing the hydrogen productivity from sugarcane bagasse is feasible by incorporation of paper mill sludge and sulfonated graphene catalyst at dosage not exceeding 60 mgSGR/gVS.					
36372032	5	1	theme	bagasse	858:864	arg1	hydrogenation					831:843	The hydrogenation	827:843	The hydrogenation of sugarcane bagasse	827:864	The hydrogenation of sugarcane bagasse was maximized at a sulfonated graphene catalyst dosage of 60 mgSGR/gVS, providing the highest hydrogen harvesting of 4104 ± 321 mL.					
36372032	11	2	theme	associated	1942:1951	arg1	feasibility					1962:1972	the associated economic feasibility	1938:1972	the associated economic feasibility	1938:1972	However, investigating the recyclability and disposal of digestate containing sulfonated graphene catalyst and the associated economic feasibility needs more attention in the future.					
36372032	10	3	theme	paper	1738:1742	arg1	mill					1744:1747	paper mill sludge and sulfonated graphene catalyst	1738:1787	mill	1744:1747	Finally maximizing the hydrogen productivity from sugarcane bagasse is feasible by incorporation of paper mill sludge and sulfonated graphene catalyst at dosage not exceeding 60 mgSGR/gVS.					
36372032	1	4	theme	hydrogen	170:177	arg1	harvesting					179:188	hydrogen harvesting	170:188	hydrogen harvesting from fermentation of sugarcane bagasse (SCB)	170:233	In this study, hydrogen harvesting from fermentation of sugarcane bagasse (SCB) was promoted by maintaining synergism between sulfonated graphene (SGR) catalyst and paper mill sludge (PMS).					
36372032	9	5	theme	dosage	1406:1411	arg1	mgSGR/gVS					1423:1431	the catalyst dosage up to 120 mgSGR/gVS	1393:1431	the catalyst dosage up to 120 mgSGR/gVS	1393:1431	Nevertheless, elevating the catalyst dosage up to 120 mgSGR/gVS reduced the hydrogen harvesting (1190 ± 92 mL) due to the release of furfural (1.76 ± 0.02 g/L) in the fermentation cultures, deteriorating the microbes' internal composition and metabolism bioactivities.					
36372032	2	6	theme	biodegradable	496:508	arg1	sugars					510:515	readily biodegradable sugars	488:515	readily biodegradable sugars into the fermentation medium	488:544	The sulfonic acid (-SO3H) groups in the catalyst played a major role in destructing the β-1,4 glycosidic bonds of sugarcane bagasse, releasing readily biodegradable sugars into the fermentation medium.					
36372032	6	7	dep	%	1073:1073	arg1	up					1063:1064	up	1063:1064	up	1063:1064	This was associated with an increase of the Proteobacteria phyla up to 52.0%, Firmicutes phyla to 13.9%, and Acinetobacter sp.					
36372032	11	8	theme	graphene	1916:1923	arg1	catalyst					1925:1932	sulfonated graphene catalyst	1905:1932	sulfonated graphene catalyst	1905:1932	However, investigating the recyclability and disposal of digestate containing sulfonated graphene catalyst and the associated economic feasibility needs more attention in the future.					
36372032	8	9	theme	reaction	1300:1307	arg1	pathway					1309:1315	the acetate fermentation reaction pathway	1275:1315	the acetate fermentation reaction pathway	1275:1315	Moreover, sulfonated graphene catalyst supplementation promoted the acetate fermentation reaction pathway by increasing the acetate/butyrate ratio up to 4.1.					
36372032	0	10	theme	bio-hydrogen	89:100	arg1	harvesting					102:111	bio-hydrogen harvesting	89:111	bio-hydrogen harvesting	89:111	Synergistic effects of paper mill sludge and sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane bagasse de-polymerization.					
36372032	3	11	theme	20	673:674	arg1	mgSGR/gVS					676:684	mgSGR/gVS	676:684	mgSGR/gVS	676:684	The cellulose, hemicellulose, and lignin conversion efficiency were improved by 127.5%, 495.0%, and 109.2%, respectively with 20 mgSGR/gVS catalyst addition, compared with the control samples.					
36372032	8	12	theme	catalyst	1241:1248	arg1	supplementation					1250:1264	sulfonated graphene catalyst supplementation	1221:1264	sulfonated graphene catalyst supplementation	1221:1264	Moreover, sulfonated graphene catalyst supplementation promoted the acetate fermentation reaction pathway by increasing the acetate/butyrate ratio up to 4.1.					
36372032	5	13	theme	mgSGR/gVS	927:935	arg1	dosage					914:919	a sulfonated graphene catalyst dosage	883:919	a sulfonated graphene catalyst dosage of 60 mgSGR/gVS	883:935	The hydrogenation of sugarcane bagasse was maximized at a sulfonated graphene catalyst dosage of 60 mgSGR/gVS, providing the highest hydrogen harvesting of 4104 ± 321 mL.					
36372032	10	14	dep	mill	1744:1747	arg1	sludge					1749:1754	sludge	1749:1754	sludge	1749:1754	Finally maximizing the hydrogen productivity from sugarcane bagasse is feasible by incorporation of paper mill sludge and sulfonated graphene catalyst at dosage not exceeding 60 mgSGR/gVS.					
36372032	1	15	theme	sulfonated	281:290	arg1	catalyst					307:314	sulfonated graphene (SGR) catalyst	281:314	sulfonated graphene (SGR) catalyst	281:314	In this study, hydrogen harvesting from fermentation of sugarcane bagasse (SCB) was promoted by maintaining synergism between sulfonated graphene (SGR) catalyst and paper mill sludge (PMS).					
36372032	3	16	theme	mgSGR/gVS	676:684	arg1	addition					695:702	20 mgSGR/gVS catalyst addition	673:702	20 mgSGR/gVS catalyst addition	673:702	The cellulose, hemicellulose, and lignin conversion efficiency were improved by 127.5%, 495.0%, and 109.2%, respectively with 20 mgSGR/gVS catalyst addition, compared with the control samples.					
36372032	9	17	theme	internal	1587:1594	arg1	composition					1596:1606	the microbes' internal composition	1573:1606	the microbes' internal composition	1573:1606	Nevertheless, elevating the catalyst dosage up to 120 mgSGR/gVS reduced the hydrogen harvesting (1190 ± 92 mL) due to the release of furfural (1.76 ± 0.02 g/L) in the fermentation cultures, deteriorating the microbes' internal composition and metabolism bioactivities.					
36372032	9	18	theme	catalyst	1397:1404	arg1	mgSGR/gVS					1423:1431	the catalyst dosage up to 120 mgSGR/gVS	1393:1431	the catalyst dosage up to 120 mgSGR/gVS	1393:1431	Nevertheless, elevating the catalyst dosage up to 120 mgSGR/gVS reduced the hydrogen harvesting (1190 ± 92 mL) due to the release of furfural (1.76 ± 0.02 g/L) in the fermentation cultures, deteriorating the microbes' internal composition and metabolism bioactivities.					
36372032	6	19	theme	Acinetobacter	1107:1119	arg1	sp					1121:1122	Acinetobacter sp	1107:1122	Acinetobacter sp	1107:1122	This was associated with an increase of the Proteobacteria phyla up to 52.0%, Firmicutes phyla to 13.9%, and Acinetobacter sp.					
36372032	6	20	theme	Proteobacteria	1042:1055	arg1	phyla					1057:1061	the Proteobacteria phyla	1038:1061	the Proteobacteria phyla	1038:1061	This was associated with an increase of the Proteobacteria phyla up to 52.0%, Firmicutes phyla to 13.9%, and Acinetobacter sp.					
36372032	11	21	theme	economic	1953:1960	arg1	feasibility					1962:1972	the associated economic feasibility	1938:1972	the associated economic feasibility	1938:1972	However, investigating the recyclability and disposal of digestate containing sulfonated graphene catalyst and the associated economic feasibility needs more attention in the future.					
36372032	9	22	theme	fermentation	1536:1547	arg1	cultures					1549:1556	the fermentation cultures	1532:1556	the fermentation cultures	1532:1556	Nevertheless, elevating the catalyst dosage up to 120 mgSGR/gVS reduced the hydrogen harvesting (1190 ± 92 mL) due to the release of furfural (1.76 ± 0.02 g/L) in the fermentation cultures, deteriorating the microbes' internal composition and metabolism bioactivities.					
36372032	9	23	dep	120	1419:1421	arg1	to					1416:1417	to	1416:1417	to	1416:1417	Nevertheless, elevating the catalyst dosage up to 120 mgSGR/gVS reduced the hydrogen harvesting (1190 ± 92 mL) due to the release of furfural (1.76 ± 0.02 g/L) in the fermentation cultures, deteriorating the microbes' internal composition and metabolism bioactivities.					
36372032	11	24	theme	more	1980:1983	arg1	attention					1985:1993	more attention	1980:1993	more attention	1980:1993	However, investigating the recyclability and disposal of digestate containing sulfonated graphene catalyst and the associated economic feasibility needs more attention in the future.					
36372032	4	25	theme	graphene	808:815	arg1	catalyst					817:824	non-sulfonated graphene catalyst	793:824	non-sulfonated graphene catalyst	793:824	These values were also higher than those obtained by non-sulfonated graphene catalyst.					
36372032	0	26	theme	catalyst	65:72	arg1	effects					12:18	Synergistic effects	0:18	Synergistic effects of paper mill sludge and sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane	0:126	Synergistic effects of paper mill sludge and sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane bagasse de-polymerization.					
36372032	5	27	theme	graphene	896:903	arg1	dosage					914:919	a sulfonated graphene catalyst dosage	883:919	a sulfonated graphene catalyst dosage of 60 mgSGR/gVS	883:935	The hydrogenation of sugarcane bagasse was maximized at a sulfonated graphene catalyst dosage of 60 mgSGR/gVS, providing the highest hydrogen harvesting of 4104 ± 321 mL.					
36372032	0	28	theme	Synergistic	0:10	arg1	effects					12:18	Synergistic effects	0:18	Synergistic effects of paper mill sludge and sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane	0:126	Synergistic effects of paper mill sludge and sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane bagasse de-polymerization.					
36372032	1	29	theme	graphene	292:299	arg1	catalyst					307:314	sulfonated graphene (SGR) catalyst	281:314	sulfonated graphene (SGR) catalyst	281:314	In this study, hydrogen harvesting from fermentation of sugarcane bagasse (SCB) was promoted by maintaining synergism between sulfonated graphene (SGR) catalyst and paper mill sludge (PMS).					
36372032	4	30	theme	non-sulfonated	793:806	arg1	catalyst					817:824	non-sulfonated graphene catalyst	793:824	non-sulfonated graphene catalyst	793:824	These values were also higher than those obtained by non-sulfonated graphene catalyst.					
36372032	5	31	theme	catalyst	905:912	arg1	dosage					914:919	a sulfonated graphene catalyst dosage	883:919	a sulfonated graphene catalyst dosage of 60 mgSGR/gVS	883:935	The hydrogenation of sugarcane bagasse was maximized at a sulfonated graphene catalyst dosage of 60 mgSGR/gVS, providing the highest hydrogen harvesting of 4104 ± 321 mL.					
36372032	9	32	theme	hydrogen	1445:1452	arg1	1190 ± 92 mL					1466:1477	1190 ± 92 mL	1466:1477	1190 ± 92 mL	1466:1477	Nevertheless, elevating the catalyst dosage up to 120 mgSGR/gVS reduced the hydrogen harvesting (1190 ± 92 mL) due to the release of furfural (1.76 ± 0.02 g/L) in the fermentation cultures, deteriorating the microbes' internal composition and metabolism bioactivities.					
36372032	9	32	theme	hydrogen	1445:1452	arg1	harvesting					1454:1463	the hydrogen harvesting	1441:1463	the hydrogen harvesting (1190 ± 92 mL)	1441:1478	Nevertheless, elevating the catalyst dosage up to 120 mgSGR/gVS reduced the hydrogen harvesting (1190 ± 92 mL) due to the release of furfural (1.76 ± 0.02 g/L) in the fermentation cultures, deteriorating the microbes' internal composition and metabolism bioactivities.					
36372032	2	33	theme	major	403:407	arg1	role					409:412	a major role	401:412	a major role	401:412	The sulfonic acid (-SO3H) groups in the catalyst played a major role in destructing the β-1,4 glycosidic bonds of sugarcane bagasse, releasing readily biodegradable sugars into the fermentation medium.					
36372032	5	34	theme	hydrogen	960:967	arg1	harvesting					969:978	the highest hydrogen harvesting	948:978	the highest hydrogen harvesting of 4104 ± 321 mL	948:995	The hydrogenation of sugarcane bagasse was maximized at a sulfonated graphene catalyst dosage of 60 mgSGR/gVS, providing the highest hydrogen harvesting of 4104 ± 321 mL.					
36372032	2	35	theme	fermentation	526:537	arg1	medium					539:544	the fermentation medium	522:544	the fermentation medium	522:544	The sulfonic acid (-SO3H) groups in the catalyst played a major role in destructing the β-1,4 glycosidic bonds of sugarcane bagasse, releasing readily biodegradable sugars into the fermentation medium.					
36372032	5	36	theme	sulfonated	885:894	arg1	dosage					914:919	a sulfonated graphene catalyst dosage	883:919	a sulfonated graphene catalyst dosage of 60 mgSGR/gVS	883:935	The hydrogenation of sugarcane bagasse was maximized at a sulfonated graphene catalyst dosage of 60 mgSGR/gVS, providing the highest hydrogen harvesting of 4104 ± 321 mL.					
36372032	8	37	theme	sulfonated	1221:1230	arg1	supplementation					1250:1264	sulfonated graphene catalyst supplementation	1221:1264	sulfonated graphene catalyst supplementation	1221:1264	Moreover, sulfonated graphene catalyst supplementation promoted the acetate fermentation reaction pathway by increasing the acetate/butyrate ratio up to 4.1.					
36372032	10	38	theme	sulfonated	1760:1769	arg1	catalyst					1780:1787	sulfonated graphene catalyst	1760:1787	sulfonated graphene catalyst	1760:1787	Finally maximizing the hydrogen productivity from sugarcane bagasse is feasible by incorporation of paper mill sludge and sulfonated graphene catalyst at dosage not exceeding 60 mgSGR/gVS.					
36372032	1	39	theme	sugarcane	211:219	arg1	bagasse					221:227	sugarcane bagasse	211:227	sugarcane bagasse (SCB)	211:233	In this study, hydrogen harvesting from fermentation of sugarcane bagasse (SCB) was promoted by maintaining synergism between sulfonated graphene (SGR) catalyst and paper mill sludge (PMS).					
36372032	1	39	theme	sugarcane	211:219	arg1	SCB					230:232	SCB	230:232	SCB	230:232	In this study, hydrogen harvesting from fermentation of sugarcane bagasse (SCB) was promoted by maintaining synergism between sulfonated graphene (SGR) catalyst and paper mill sludge (PMS).					
36372032	8	40	theme	acetate	1279:1285	arg1	pathway					1309:1315	the acetate fermentation reaction pathway	1275:1315	the acetate fermentation reaction pathway	1275:1315	Moreover, sulfonated graphene catalyst supplementation promoted the acetate fermentation reaction pathway by increasing the acetate/butyrate ratio up to 4.1.					
36372032	3	41	theme	catalyst	686:693	arg1	addition					695:702	20 mgSGR/gVS catalyst addition	673:702	20 mgSGR/gVS catalyst addition	673:702	The cellulose, hemicellulose, and lignin conversion efficiency were improved by 127.5%, 495.0%, and 109.2%, respectively with 20 mgSGR/gVS catalyst addition, compared with the control samples.					
36372032	10	42	theme	graphene	1771:1778	arg1	catalyst					1780:1787	sulfonated graphene catalyst	1760:1787	sulfonated graphene catalyst	1760:1787	Finally maximizing the hydrogen productivity from sugarcane bagasse is feasible by incorporation of paper mill sludge and sulfonated graphene catalyst at dosage not exceeding 60 mgSGR/gVS.					
36372032	6	43	theme	phyla	1057:1061	arg1	sp					1121:1122	Acinetobacter sp	1107:1122	Acinetobacter sp	1107:1122	This was associated with an increase of the Proteobacteria phyla up to 52.0%, Firmicutes phyla to 13.9%, and Acinetobacter sp.					
36372032	6	43	theme	phyla	1057:1061	arg1	increase					1026:1033	an increase	1023:1033	an increase of the Proteobacteria phyla up to 52.0%	1023:1073	This was associated with an increase of the Proteobacteria phyla up to 52.0%, Firmicutes phyla to 13.9%, and Acinetobacter sp.					
36372032	6	43	theme	phyla	1057:1061	arg1	phyla					1087:1091	Firmicutes phyla	1076:1091	Firmicutes phyla to 13.9%	1076:1100	This was associated with an increase of the Proteobacteria phyla up to 52.0%, Firmicutes phyla to 13.9%, and Acinetobacter sp.					
36372032	10	44	from	dosage	1792:1797	arg1	incorporation					1721:1733	incorporation	1721:1733	incorporation of paper mill sludge and sulfonated graphene catalyst at dosage not exceeding 60 mgSGR/gVS	1721:1824	Finally maximizing the hydrogen productivity from sugarcane bagasse is feasible by incorporation of paper mill sludge and sulfonated graphene catalyst at dosage not exceeding 60 mgSGR/gVS.					
36372032	1	45	theme	bagasse	221:227	arg1	fermentation					195:206	fermentation	195:206	fermentation of sugarcane bagasse (SCB)	195:233	In this study, hydrogen harvesting from fermentation of sugarcane bagasse (SCB) was promoted by maintaining synergism between sulfonated graphene (SGR) catalyst and paper mill sludge (PMS).					
36372032	2	46	theme	glycosidic	439:448	arg1	bonds					450:454	the β-1,4 glycosidic bonds	429:454	the β-1,4 glycosidic bonds of sugarcane bagasse	429:475	The sulfonic acid (-SO3H) groups in the catalyst played a major role in destructing the β-1,4 glycosidic bonds of sugarcane bagasse, releasing readily biodegradable sugars into the fermentation medium.					
36372032	9	47	theme	metabolism	1612:1621	arg1	bioactivities					1623:1635	metabolism bioactivities	1612:1635	metabolism bioactivities	1612:1635	Nevertheless, elevating the catalyst dosage up to 120 mgSGR/gVS reduced the hydrogen harvesting (1190 ± 92 mL) due to the release of furfural (1.76 ± 0.02 g/L) in the fermentation cultures, deteriorating the microbes' internal composition and metabolism bioactivities.					
36372032	10	48	theme	mill	1744:1747	arg1	incorporation					1721:1733	incorporation	1721:1733	incorporation of paper mill sludge and sulfonated graphene catalyst at dosage not exceeding 60 mgSGR/gVS	1721:1824	Finally maximizing the hydrogen productivity from sugarcane bagasse is feasible by incorporation of paper mill sludge and sulfonated graphene catalyst at dosage not exceeding 60 mgSGR/gVS.					
36372032	7	49	theme	control	1183:1189	arg1	assay					1191:1195	the control assay	1179:1195	the control assay respectively	1179:1208	to 39.8% compared with only 37.0%, 11.3% and 11.1% in the control assay respectively.					
36372032	0	50	theme	mill	29:32	arg1	effects					12:18	Synergistic effects	0:18	Synergistic effects of paper mill sludge and sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane	0:126	Synergistic effects of paper mill sludge and sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane bagasse de-polymerization.					
36372032	3	51	theme	control	723:729	arg1	samples					731:737	the control samples	719:737	the control samples	719:737	The cellulose, hemicellulose, and lignin conversion efficiency were improved by 127.5%, 495.0%, and 109.2%, respectively with 20 mgSGR/gVS catalyst addition, compared with the control samples.					
36372032	2	52	theme	β-1,4	433:437	arg1	bonds					450:454	the β-1,4 glycosidic bonds	429:454	the β-1,4 glycosidic bonds of sugarcane bagasse	429:475	The sulfonic acid (-SO3H) groups in the catalyst played a major role in destructing the β-1,4 glycosidic bonds of sugarcane bagasse, releasing readily biodegradable sugars into the fermentation medium.					
36372032	0	53	theme	paper	23:27	arg1	mill					29:32	paper mill sludge and sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane	23:126	mill	29:32	Synergistic effects of paper mill sludge and sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane bagasse de-polymerization.					
36372032	11	54	theme	digestate	1884:1892	arg1	disposal					1872:1879	disposal	1872:1879	disposal	1872:1879	However, investigating the recyclability and disposal of digestate containing sulfonated graphene catalyst and the associated economic feasibility needs more attention in the future.					
36372032	11	54	theme	digestate	1884:1892	arg1	recyclability					1854:1866	recyclability	1854:1866	recyclability	1854:1866	However, investigating the recyclability and disposal of digestate containing sulfonated graphene catalyst and the associated economic feasibility needs more attention in the future.					
36372032	0	55	theme	sulfonated	45:54	arg1	catalyst					65:72	sulfonated graphene catalyst	45:72	sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane	45:126	Synergistic effects of paper mill sludge and sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane bagasse de-polymerization.					
36372032	8	56	theme	fermentation	1287:1298	arg1	pathway					1309:1315	the acetate fermentation reaction pathway	1275:1315	the acetate fermentation reaction pathway	1275:1315	Moreover, sulfonated graphene catalyst supplementation promoted the acetate fermentation reaction pathway by increasing the acetate/butyrate ratio up to 4.1.					
36372032	0	57	theme	graphene	56:63	arg1	catalyst					65:72	sulfonated graphene catalyst	45:72	sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane	45:126	Synergistic effects of paper mill sludge and sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane bagasse de-polymerization.					
36372032	8	58	theme	acetate/butyrate	1335:1350	arg1	ratio					1352:1356	the acetate/butyrate ratio up to 4.1	1331:1366	the acetate/butyrate ratio up to 4.1	1331:1366	Moreover, sulfonated graphene catalyst supplementation promoted the acetate fermentation reaction pathway by increasing the acetate/butyrate ratio up to 4.1.					
36372032	8	59	dep	4.1	1364:1366	arg1	to					1361:1362	to	1361:1362	to	1361:1362	Moreover, sulfonated graphene catalyst supplementation promoted the acetate fermentation reaction pathway by increasing the acetate/butyrate ratio up to 4.1.					
36372032	1	60	theme	paper	320:324	arg1	mill					326:329	paper mill	320:329	paper mill sludge (PMS)	320:342	In this study, hydrogen harvesting from fermentation of sugarcane bagasse (SCB) was promoted by maintaining synergism between sulfonated graphene (SGR) catalyst and paper mill sludge (PMS).					
36372032	0	61	dep	mill	29:32	arg1	sludge					34:39	sludge	34:39	sludge	34:39	Synergistic effects of paper mill sludge and sulfonated graphene catalyst for maximizing bio-hydrogen harvesting from sugarcane bagasse de-polymerization.					
36372032	2	62	from	groups	371:376	arg1	catalyst					385:392	the catalyst	381:392	the catalyst	381:392	The sulfonic acid (-SO3H) groups in the catalyst played a major role in destructing the β-1,4 glycosidic bonds of sugarcane bagasse, releasing readily biodegradable sugars into the fermentation medium.					
36372032	6	63	theme	Firmicutes	1076:1085	arg1	phyla					1087:1091	Firmicutes phyla	1076:1091	Firmicutes phyla to 13.9%	1076:1100	This was associated with an increase of the Proteobacteria phyla up to 52.0%, Firmicutes phyla to 13.9%, and Acinetobacter sp.					
36372032	1	64	theme	mill	326:329	arg1	PMS					339:341	PMS	339:341	PMS	339:341	In this study, hydrogen harvesting from fermentation of sugarcane bagasse (SCB) was promoted by maintaining synergism between sulfonated graphene (SGR) catalyst and paper mill sludge (PMS).					
36372032	1	64	theme	mill	326:329	arg1	sludge					331:336	paper mill sludge	320:336	paper mill sludge (PMS)	320:342	In this study, hydrogen harvesting from fermentation of sugarcane bagasse (SCB) was promoted by maintaining synergism between sulfonated graphene (SGR) catalyst and paper mill sludge (PMS).					
36372032	5	65	theme	4104 ± 321 mL	983:995	arg1	harvesting					969:978	the highest hydrogen harvesting	948:978	the highest hydrogen harvesting of 4104 ± 321 mL	948:995	The hydrogenation of sugarcane bagasse was maximized at a sulfonated graphene catalyst dosage of 60 mgSGR/gVS, providing the highest hydrogen harvesting of 4104 ± 321 mL.					
36372032	9	66	theme	furfural	1502:1509	arg1	release					1491:1497	the release	1487:1497	the release of furfural (1.76 ± 0.02 g/L) in the fermentation cultures	1487:1556	Nevertheless, elevating the catalyst dosage up to 120 mgSGR/gVS reduced the hydrogen harvesting (1190 ± 92 mL) due to the release of furfural (1.76 ± 0.02 g/L) in the fermentation cultures, deteriorating the microbes' internal composition and metabolism bioactivities.					
36372032	10	67	theme	hydrogen	1661:1668	arg1	productivity					1670:1681	the hydrogen productivity	1657:1681	the hydrogen productivity	1657:1681	Finally maximizing the hydrogen productivity from sugarcane bagasse is feasible by incorporation of paper mill sludge and sulfonated graphene catalyst at dosage not exceeding 60 mgSGR/gVS.					
36372032	10	68	theme	sugarcane	1688:1696	arg1	bagasse					1698:1704	sugarcane bagasse	1688:1704	sugarcane bagasse	1688:1704	Finally maximizing the hydrogen productivity from sugarcane bagasse is feasible by incorporation of paper mill sludge and sulfonated graphene catalyst at dosage not exceeding 60 mgSGR/gVS.					
36372032	11	69	dep	recyclability	1854:1866	arg1	the					1850:1852	the	1850:1852	the	1850:1852	However, investigating the recyclability and disposal of digestate containing sulfonated graphene catalyst and the associated economic feasibility needs more attention in the future.					
36372032	5	70	theme	highest	952:958	arg1	harvesting					969:978	the highest hydrogen harvesting	948:978	the highest hydrogen harvesting of 4104 ± 321 mL	948:995	The hydrogenation of sugarcane bagasse was maximized at a sulfonated graphene catalyst dosage of 60 mgSGR/gVS, providing the highest hydrogen harvesting of 4104 ± 321 mL.					
36372032	1	71	from	fermentation	195:206	arg1	harvesting					179:188	hydrogen harvesting	170:188	hydrogen harvesting from fermentation of sugarcane bagasse (SCB)	170:233	In this study, hydrogen harvesting from fermentation of sugarcane bagasse (SCB) was promoted by maintaining synergism between sulfonated graphene (SGR) catalyst and paper mill sludge (PMS).					
36372032	5	72	theme	sugarcane	848:856	arg1	bagasse					858:864	sugarcane bagasse	848:864	sugarcane bagasse	848:864	The hydrogenation of sugarcane bagasse was maximized at a sulfonated graphene catalyst dosage of 60 mgSGR/gVS, providing the highest hydrogen harvesting of 4104 ± 321 mL.					
36372032	8	73	theme	graphene	1232:1239	arg1	supplementation					1250:1264	sulfonated graphene catalyst supplementation	1221:1264	sulfonated graphene catalyst supplementation	1221:1264	Moreover, sulfonated graphene catalyst supplementation promoted the acetate fermentation reaction pathway by increasing the acetate/butyrate ratio up to 4.1.					
36372032	2	74	theme	bagasse	469:475	arg1	bonds					450:454	the β-1,4 glycosidic bonds	429:454	the β-1,4 glycosidic bonds of sugarcane bagasse	429:475	The sulfonic acid (-SO3H) groups in the catalyst played a major role in destructing the β-1,4 glycosidic bonds of sugarcane bagasse, releasing readily biodegradable sugars into the fermentation medium.					
36372032	11	75	dep	needs	1974:1978	arg1	investigating					1836:1848	investigating	1836:1848	investigating the recyclability and disposal of digestate containing sulfonated graphene catalyst	1836:1932	However, investigating the recyclability and disposal of digestate containing sulfonated graphene catalyst and the associated economic feasibility needs more attention in the future.					
36372032	3	76	theme	lignin	581:586	arg1	efficiency					599:608	lignin conversion efficiency	581:608	lignin conversion efficiency	581:608	The cellulose, hemicellulose, and lignin conversion efficiency were improved by 127.5%, 495.0%, and 109.2%, respectively with 20 mgSGR/gVS catalyst addition, compared with the control samples.					
36372032	2	77	theme	acid	358:361	arg1	groups					371:376	The sulfonic acid (-SO3H) groups	345:376	The sulfonic acid (-SO3H) groups in the catalyst	345:392	The sulfonic acid (-SO3H) groups in the catalyst played a major role in destructing the β-1,4 glycosidic bonds of sugarcane bagasse, releasing readily biodegradable sugars into the fermentation medium.					
36372032	9	78	from	release	1491:1497	arg1	cultures					1549:1556	the fermentation cultures	1532:1556	the fermentation cultures	1532:1556	Nevertheless, elevating the catalyst dosage up to 120 mgSGR/gVS reduced the hydrogen harvesting (1190 ± 92 mL) due to the release of furfural (1.76 ± 0.02 g/L) in the fermentation cultures, deteriorating the microbes' internal composition and metabolism bioactivities.					
36372032	2	79	theme	sugarcane	459:467	arg1	bagasse					469:475	sugarcane bagasse	459:475	sugarcane bagasse	459:475	The sulfonic acid (-SO3H) groups in the catalyst played a major role in destructing the β-1,4 glycosidic bonds of sugarcane bagasse, releasing readily biodegradable sugars into the fermentation medium.					
36372032	11	80	theme	sulfonated	1905:1914	arg1	catalyst					1925:1932	sulfonated graphene catalyst	1905:1932	sulfonated graphene catalyst	1905:1932	However, investigating the recyclability and disposal of digestate containing sulfonated graphene catalyst and the associated economic feasibility needs more attention in the future.					
36372032	3	81	theme	conversion	588:597	arg1	efficiency					599:608	lignin conversion efficiency	581:608	lignin conversion efficiency	581:608	The cellulose, hemicellulose, and lignin conversion efficiency were improved by 127.5%, 495.0%, and 109.2%, respectively with 20 mgSGR/gVS catalyst addition, compared with the control samples.					
36372032	11	82	contain	containing	1894:1903	arg1	digestate					1884:1892	digestate	1884:1892	digestate containing sulfonated graphene catalyst	1884:1932	However, investigating the recyclability and disposal of digestate containing sulfonated graphene catalyst and the associated economic feasibility needs more attention in the future.					
36372032	11	82	contain	containing	1894:1903	arg2	catalyst					1925:1932	sulfonated graphene catalyst	1905:1932	sulfonated graphene catalyst	1905:1932	However, investigating the recyclability and disposal of digestate containing sulfonated graphene catalyst and the associated economic feasibility needs more attention in the future.					
36372032	2	83	theme	sulfonic	349:356	arg1	-SO3H					364:368	-SO3H	364:368	-SO3H	364:368	The sulfonic acid (-SO3H) groups in the catalyst played a major role in destructing the β-1,4 glycosidic bonds of sugarcane bagasse, releasing readily biodegradable sugars into the fermentation medium.					
36372032	2	83	theme	sulfonic	349:356	arg1	acid					358:361	sulfonic acid	349:361	The sulfonic acid (-SO3H) groups in the catalyst	345:392	The sulfonic acid (-SO3H) groups in the catalyst played a major role in destructing the β-1,4 glycosidic bonds of sugarcane bagasse, releasing readily biodegradable sugars into the fermentation medium.					
35651090	0	0	theme	Aspergillus	88:98	arg1	whole-cells					106:116	Aspergillus niger whole-cells	88:116	Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids	88:184	Comparative transcriptomics to reveal the mechanism of enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids.					
35651090	11	1	theme	whole-cell	1673:1682	arg1	catalyst					1684:1691	whole-cell catalyst	1673:1691	whole-cell catalyst	1673:1691	This study gave a new approach to hydrolyze HES, and new perspectives to understand the mechanisms associated with the hydrolysis of whole-cell catalyst.					
35651090	10	2	theme	real-time	1415:1423	arg1	qRT-PCR					1452:1458	qRT-PCR	1452:1458	qRT-PCR	1452:1458	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the results of transcriptome sequencing were reliable.					
35651090	10	2	theme	real-time	1415:1423	arg1	reaction					1442:1449	real-time polymerase chain reaction	1415:1449	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis	1398:1468	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the results of transcriptome sequencing were reliable.					
35651090	7	3	dep	Ontology	975:982	arg1	GO					985:986	GO	985:986	GO	985:986	The Gene Ontology (GO) analysis showed that whether naringin or saccharose was added as an inducer would greatly affect the catalytic activity of cells.					
35651090	1	4	theme	hesperidin	274:283	arg1	hydrolysis					260:269	the cascade hydrolysis	248:269	the cascade hydrolysis of hesperidin (HES)	248:289	A new Aspergillus niger whole-cell catalyst was cultured for the cascade hydrolysis of hesperidin (HES) to produce high-value hesperetin-7-O-glucoside (HG) and hesperetin with high conversion (above 90%).					
35651090	0	5	theme	whole-cells	106:116	arg1	activities					74:83	enhanced catalytic activities	55:83	enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids	55:184	Comparative transcriptomics to reveal the mechanism of enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids.					
35651090	1	6	theme	high	363:366	arg1	%					388:388	above 90%	380:388	above 90%	380:388	A new Aspergillus niger whole-cell catalyst was cultured for the cascade hydrolysis of hesperidin (HES) to produce high-value hesperetin-7-O-glucoside (HG) and hesperetin with high conversion (above 90%).					
35651090	1	6	theme	high	363:366	arg1	conversion					368:377	high conversion	363:377	high conversion (above 90%)	363:389	A new Aspergillus niger whole-cell catalyst was cultured for the cascade hydrolysis of hesperidin (HES) to produce high-value hesperetin-7-O-glucoside (HG) and hesperetin with high conversion (above 90%).					
35651090	10	7	theme	quantitative	1402:1413	arg1	analysis					1461:1468	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis	1398:1468	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis	1398:1468	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the results of transcriptome sequencing were reliable.					
35651090	9	8	theme	glucosidase	1306:1316	arg1	activities					1318:1327	glucosidase activities	1306:1327	glucosidase activities	1306:1327	These genes were not only involved in rhamnosidase and glucosidase activities, but also spliceosome and the sucrose and starch metabolic pathways.					
35651090	0	9	theme	niger	100:104	arg1	whole-cells					106:116	Aspergillus niger whole-cells	88:116	Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids	88:184	Comparative transcriptomics to reveal the mechanism of enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids.					
35651090	9	10	theme	starch	1371:1376	arg1	pathways					1388:1395	the sucrose and starch metabolic pathways	1355:1395	pathways	1388:1395	These genes were not only involved in rhamnosidase and glucosidase activities, but also spliceosome and the sucrose and starch metabolic pathways.					
35651090	5	11	theme	saccharose-vs-blank	740:758	arg1	group					760:764	saccharose-vs-blank group	740:764	saccharose-vs-blank group	740:764	Transcriptome analysis suggested that both naringin-vs-blank group and saccharose-vs-blank group had obviously difference in gene expression.					
35651090	6	12	theme	naringin-vs-blank	815:831	arg1	group					833:837	The naringin-vs-blank group	811:837	The naringin-vs-blank group	811:837	The naringin-vs-blank group was mainly up-regulated differentially expressed genes (DEGs), while saccharose-vs-blank group was mainly down-regulated DEGs.					
35651090	4	13	theme	different	649:657	arg1	inducers					659:666	different inducers	649:666	different inducers	649:666	The product composition was also controllable by adjusting different inducers.					
35651090	6	14	theme	saccharose-vs-blank	908:926	arg1	group					928:932	saccharose-vs-blank group	908:932	saccharose-vs-blank group	908:932	The naringin-vs-blank group was mainly up-regulated differentially expressed genes (DEGs), while saccharose-vs-blank group was mainly down-regulated DEGs.					
35651090	8	15	theme	hydrolase	1220:1228	arg1	activity					1230:1237	hydrolase activity	1220:1237	hydrolase activity	1220:1237	Furthermore, 3 genes related to rhamnosidase, 14 genes related to glucosidase and 5 genes related to hydrolase activity were found.					
35651090	7	16	theme	Ontology	975:982	arg1	analysis					989:996	The Gene Ontology (GO) analysis	966:996	The Gene Ontology (GO) analysis	966:996	The Gene Ontology (GO) analysis showed that whether naringin or saccharose was added as an inducer would greatly affect the catalytic activity of cells.					
35651090	1	17	theme	new	189:191	arg1	niger					205:209	A new Aspergillus niger	187:209	A new Aspergillus niger whole-cell catalyst	187:229	A new Aspergillus niger whole-cell catalyst was cultured for the cascade hydrolysis of hesperidin (HES) to produce high-value hesperetin-7-O-glucoside (HG) and hesperetin with high conversion (above 90%).					
35651090	10	18	theme	reaction	1442:1449	arg1	analysis					1461:1468	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis	1398:1468	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis	1398:1468	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the results of transcriptome sequencing were reliable.					
35651090	3	19	theme	inducers	533:540	arg1	type					525:528	the type	521:528	the type of inducers	521:540	Remarkably, the type of inducers determined whether the cells can hydrolyze HES.					
35651090	1	20	theme	Aspergillus	193:203	arg1	niger					205:209	A new Aspergillus niger	187:209	A new Aspergillus niger whole-cell catalyst	187:229	A new Aspergillus niger whole-cell catalyst was cultured for the cascade hydrolysis of hesperidin (HES) to produce high-value hesperetin-7-O-glucoside (HG) and hesperetin with high conversion (above 90%).					
35651090	0	21	theme	Comparative	0:10	arg1	transcriptomics					12:26	Comparative transcriptomics	0:26	Comparative transcriptomics	0:26	Comparative transcriptomics to reveal the mechanism of enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids.					
35651090	7	22	theme	cells	1112:1116	arg1	activity					1100:1107	the catalytic activity	1086:1107	the catalytic activity of cells	1086:1116	The Gene Ontology (GO) analysis showed that whether naringin or saccharose was added as an inducer would greatly affect the catalytic activity of cells.					
35651090	10	23	theme	chain	1436:1440	arg1	qRT-PCR					1452:1458	qRT-PCR	1452:1458	qRT-PCR	1452:1458	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the results of transcriptome sequencing were reliable.					
35651090	10	23	theme	chain	1436:1440	arg1	reaction					1442:1449	real-time polymerase chain reaction	1415:1449	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis	1398:1468	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the results of transcriptome sequencing were reliable.					
35651090	1	24	theme	niger	205:209	arg1	catalyst					222:229	A new Aspergillus niger whole-cell catalyst	187:229	A new Aspergillus niger whole-cell catalyst	187:229	A new Aspergillus niger whole-cell catalyst was cultured for the cascade hydrolysis of hesperidin (HES) to produce high-value hesperetin-7-O-glucoside (HG) and hesperetin with high conversion (above 90%).					
35651090	8	25	theme	related	1209:1215	arg1	genes					1203:1207	5 genes	1201:1207	5 genes related to hydrolase activity	1201:1237	Furthermore, 3 genes related to rhamnosidase, 14 genes related to glucosidase and 5 genes related to hydrolase activity were found.					
35651090	10	26	theme	transcriptome	1500:1512	arg1	sequencing					1514:1523	transcriptome sequencing	1500:1523	transcriptome sequencing	1500:1523	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the results of transcriptome sequencing were reliable.					
35651090	7	27	theme	catalytic	1090:1098	arg1	activity					1100:1107	the catalytic activity	1086:1107	the catalytic activity of cells	1086:1116	The Gene Ontology (GO) analysis showed that whether naringin or saccharose was added as an inducer would greatly affect the catalytic activity of cells.					
35651090	7	28	theme	Gene	970:973	arg1	Ontology					975:982	Gene Ontology	970:982	The Gene Ontology (GO) analysis	966:996	The Gene Ontology (GO) analysis showed that whether naringin or saccharose was added as an inducer would greatly affect the catalytic activity of cells.					
35651090	1	29	theme	whole-cell	211:220	arg1	catalyst					222:229	A new Aspergillus niger whole-cell catalyst	187:229	A new Aspergillus niger whole-cell catalyst	187:229	A new Aspergillus niger whole-cell catalyst was cultured for the cascade hydrolysis of hesperidin (HES) to produce high-value hesperetin-7-O-glucoside (HG) and hesperetin with high conversion (above 90%).					
35651090	0	30	theme	different	132:140	arg1	inducers					142:149	different inducers	132:149	different inducers	132:149	Comparative transcriptomics to reveal the mechanism of enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids.					
35651090	4	31	theme	product	594:600	arg1	composition					602:612	The product composition	590:612	The product composition	590:612	The product composition was also controllable by adjusting different inducers.					
35651090	4	31	theme	product	594:600	arg1	controllable					623:634	controllable	623:634	controllable	623:634	The product composition was also controllable by adjusting different inducers.					
35651090	5	32	theme	gene	794:797	arg1	expression					799:808	gene expression	794:808	gene expression	794:808	Transcriptome analysis suggested that both naringin-vs-blank group and saccharose-vs-blank group had obviously difference in gene expression.					
35651090	1	33	theme	high-value	302:311	arg1	HG					339:340	HG	339:340	HG	339:340	A new Aspergillus niger whole-cell catalyst was cultured for the cascade hydrolysis of hesperidin (HES) to produce high-value hesperetin-7-O-glucoside (HG) and hesperetin with high conversion (above 90%).					
35651090	1	33	theme	high-value	302:311	arg1	hesperetin-7-O-glucoside					313:336	high-value hesperetin-7-O-glucoside	302:336	high-value hesperetin-7-O-glucoside (HG)	302:341	A new Aspergillus niger whole-cell catalyst was cultured for the cascade hydrolysis of hesperidin (HES) to produce high-value hesperetin-7-O-glucoside (HG) and hesperetin with high conversion (above 90%).					
35651090	10	34	theme	sequencing	1514:1523	arg1	reliable					1530:1537	reliable	1530:1537	reliable	1530:1537	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the results of transcriptome sequencing were reliable.					
35651090	10	34	theme	sequencing	1514:1523	arg1	results					1489:1495	the results	1485:1495	the results of transcriptome sequencing	1485:1523	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the results of transcriptome sequencing were reliable.					
35651090	2	35	theme	cell	448:451	arg1	growth					453:458	cell growth	448:458	cell growth	448:458	Moreover, the inducers used were shown to be useful for cell growth and to induce cells to produce specific enzymes.					
35651090	5	36	theme	naringin-vs-blank	712:728	arg1	group					730:734	naringin-vs-blank group	712:734	naringin-vs-blank group	712:734	Transcriptome analysis suggested that both naringin-vs-blank group and saccharose-vs-blank group had obviously difference in gene expression.					
35651090	0	37	theme	enhanced	55:62	arg1	activities					74:83	enhanced catalytic activities	55:83	enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids	55:184	Comparative transcriptomics to reveal the mechanism of enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids.					
35651090	8	38	theme	related	1174:1180	arg1	genes					1168:1172	14 genes	1165:1172	14 genes related to glucosidase	1165:1195	Furthermore, 3 genes related to rhamnosidase, 14 genes related to glucosidase and 5 genes related to hydrolase activity were found.					
35651090	0	39	theme	citrus	168:173	arg1	flavonoids					175:184	citrus flavonoids	168:184	citrus flavonoids	168:184	Comparative transcriptomics to reveal the mechanism of enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids.					
35651090	8	40	theme	related	1140:1146	arg1	genes					1134:1138	3 genes	1132:1138	3 genes related to rhamnosidase	1132:1162	Furthermore, 3 genes related to rhamnosidase, 14 genes related to glucosidase and 5 genes related to hydrolase activity were found.					
35651090	5	41	contain	had	766:768	arg1	group					730:734	naringin-vs-blank group	712:734	naringin-vs-blank group	712:734	Transcriptome analysis suggested that both naringin-vs-blank group and saccharose-vs-blank group had obviously difference in gene expression.					
35651090	5	41	contain	had	766:768	arg2	difference					780:789	difference	780:789	difference in gene expression	780:808	Transcriptome analysis suggested that both naringin-vs-blank group and saccharose-vs-blank group had obviously difference in gene expression.					
35651090	5	41	contain	had	766:768	arg1	group					760:764	saccharose-vs-blank group	740:764	saccharose-vs-blank group	740:764	Transcriptome analysis suggested that both naringin-vs-blank group and saccharose-vs-blank group had obviously difference in gene expression.					
35651090	9	42	theme	metabolic	1378:1386	arg1	pathways					1388:1395	the sucrose and starch metabolic pathways	1355:1395	pathways	1388:1395	These genes were not only involved in rhamnosidase and glucosidase activities, but also spliceosome and the sucrose and starch metabolic pathways.					
35651090	5	43	from	difference	780:789	arg1	expression					799:808	gene expression	794:808	gene expression	794:808	Transcriptome analysis suggested that both naringin-vs-blank group and saccharose-vs-blank group had obviously difference in gene expression.					
35651090	11	44	theme	new	1593:1595	arg1	perspectives					1597:1608	new perspectives	1593:1608	new perspectives	1593:1608	This study gave a new approach to hydrolyze HES, and new perspectives to understand the mechanisms associated with the hydrolysis of whole-cell catalyst.					
35651090	10	45	theme	polymerase	1425:1434	arg1	qRT-PCR					1452:1458	qRT-PCR	1452:1458	qRT-PCR	1452:1458	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the results of transcriptome sequencing were reliable.					
35651090	10	45	theme	polymerase	1425:1434	arg1	reaction					1442:1449	real-time polymerase chain reaction	1415:1449	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis	1398:1468	The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the results of transcriptome sequencing were reliable.					
35651090	5	46	theme	Transcriptome	669:681	arg1	analysis					683:690	Transcriptome analysis	669:690	Transcriptome analysis	669:690	Transcriptome analysis suggested that both naringin-vs-blank group and saccharose-vs-blank group had obviously difference in gene expression.					
35651090	6	47	theme	expressed	878:886	arg1	DEGs					895:898	DEGs	895:898	DEGs	895:898	The naringin-vs-blank group was mainly up-regulated differentially expressed genes (DEGs), while saccharose-vs-blank group was mainly down-regulated DEGs.					
35651090	6	47	theme	expressed	878:886	arg1	genes					888:892	differentially expressed genes	863:892	differentially expressed genes (DEGs)	863:899	The naringin-vs-blank group was mainly up-regulated differentially expressed genes (DEGs), while saccharose-vs-blank group was mainly down-regulated DEGs.					
35651090	11	48	theme	catalyst	1684:1691	arg1	hydrolysis					1659:1668	the hydrolysis	1655:1668	the hydrolysis of whole-cell catalyst	1655:1691	This study gave a new approach to hydrolyze HES, and new perspectives to understand the mechanisms associated with the hydrolysis of whole-cell catalyst.					
35651090	0	49	theme	flavonoids	175:184	arg1	hydrolysis					154:163	hydrolysis	154:163	hydrolysis of citrus flavonoids	154:184	Comparative transcriptomics to reveal the mechanism of enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids.					
35651090	11	50	theme	new	1558:1560	arg1	approach					1562:1569	a new approach	1556:1569	a new approach to hydrolyze HES, and new perspectives to understand the mechanisms associated with the hydrolysis of whole-cell catalyst	1556:1691	This study gave a new approach to hydrolyze HES, and new perspectives to understand the mechanisms associated with the hydrolysis of whole-cell catalyst.					
35651090	11	51	dep	approach	1562:1569	arg1	understand					1613:1622	understand	1613:1622	to understand the mechanisms associated with the hydrolysis of whole-cell catalyst	1610:1691	This study gave a new approach to hydrolyze HES, and new perspectives to understand the mechanisms associated with the hydrolysis of whole-cell catalyst.					
35651090	11	51	dep	approach	1562:1569	arg1	hydrolyze					1574:1582	hydrolyze	1574:1582	to hydrolyze HES	1571:1586	This study gave a new approach to hydrolyze HES, and new perspectives to understand the mechanisms associated with the hydrolysis of whole-cell catalyst.					
35651090	0	52	theme	activities	74:83	arg1	mechanism					42:50	the mechanism	38:50	the mechanism of enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids	38:184	Comparative transcriptomics to reveal the mechanism of enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids.					
35651090	1	53	theme	cascade	252:258	arg1	hydrolysis					260:269	the cascade hydrolysis	248:269	the cascade hydrolysis of hesperidin (HES)	248:289	A new Aspergillus niger whole-cell catalyst was cultured for the cascade hydrolysis of hesperidin (HES) to produce high-value hesperetin-7-O-glucoside (HG) and hesperetin with high conversion (above 90%).					
35651090	0	54	theme	catalytic	64:72	arg1	activities					74:83	enhanced catalytic activities	55:83	enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids	55:184	Comparative transcriptomics to reveal the mechanism of enhanced catalytic activities of Aspergillus niger whole-cells cultured with different inducers in hydrolysis of citrus flavonoids.					
35651090	2	55	theme	specific	491:498	arg1	enzymes					500:506	specific enzymes	491:506	specific enzymes	491:506	Moreover, the inducers used were shown to be useful for cell growth and to induce cells to produce specific enzymes.					
36371964	7	0	theme	chemometric	1314:1324	arg1	regression					1400:1409	partial least squares regression	1378:1409	partial least squares regression (PLSR)	1378:1416	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	7	0	theme	chemometric	1314:1324	arg1	analysis					1365:1372	Pearson correlation analysis	1345:1372	Pearson correlation analysis	1345:1372	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	7	0	theme	chemometric	1314:1324	arg1	methods					1326:1332	chemometric methods	1314:1332	chemometric methods	1314:1332	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	11	1	from	RND	2139:2141	arg1	RDN					2201:2203	RDN	2201:2203	RDN	2201:2203	The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.					
36371964	10	2	theme	IL-6	2028:2031	arg1	levels					2000:2005	the levels	1996:2005	the levels of TNF-α, IL-1β, and IL-6 in LPS-induced RAW264.7 cells	1996:2061	They also significantly reduced the levels of TNF-α, IL-1β, and IL-6 in LPS-induced RAW264.7 cells.					
36371964	1	3	theme	herbal	250:255	arg1	medicine					257:264	a widely used herbal medicine	236:264	a widely used herbal medicine	236:264	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	1	3	theme	herbal	250:255	arg1	rhizome					189:195	The rhizome	185:195	The rhizome of Dioscorea nipponica Makino (RDN)	185:231	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	6	4	theme	NO	1256:1257	arg1	production					1259:1268	NO production	1256:1268	NO production	1256:1268	The anti-inflammatory effects of RDN samples were then evaluated by inhibition of NO production in LPS-induced RAW264.7 cells.					
36371964	11	5	from	identification	2147:2160	arg1	RDN					2201:2203	RDN	2201:2203	RDN	2201:2203	The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.					
36371964	3	6	theme	mass	610:613	arg1	HPLC-Q/TOF-MS					629:641	HPLC-Q/TOF-MS	629:641	HPLC-Q/TOF-MS	629:641	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	3	6	theme	mass	610:613	arg1	spectrometry					615:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	545:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS)	545:642	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	3	7	theme	chromatography-quadrupole	569:593	arg1	HPLC-Q/TOF-MS					629:641	HPLC-Q/TOF-MS	629:641	HPLC-Q/TOF-MS	629:641	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	3	7	theme	chromatography-quadrupole	569:593	arg1	spectrometry					615:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	545:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS)	545:642	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	7	8	theme	bioactive	1570:1578	arg1	compounds					1580:1588	the potential anti-inflammatory bioactive compounds	1538:1588	the potential anti-inflammatory bioactive compounds of RDN	1538:1595	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	3	9	from	compounds	522:530	arg1	RDN					535:537	RDN	535:537	RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods	535:736	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	11	10	theme	components	2187:2196	arg1	identification					2147:2160	identification	2147:2160	identification	2147:2160	The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.					
36371964	11	10	theme	components	2187:2196	arg1	RND					2139:2141	RND	2139:2141	RND	2139:2141	The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.					
36371964	1	11	theme	Dioscorea	200:208	arg1	Makino					220:225	Dioscorea nipponica Makino	200:225	Dioscorea nipponica Makino (RDN)	200:231	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	1	11	theme	Dioscorea	200:208	arg1	RDN					228:230	RDN	228:230	RDN	228:230	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	9	12	theme	NO	1839:1840	arg1	production					1842:1851	NO production	1839:1851	NO production	1839:1851	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	0	13	theme	components	132:141	arg1	HPLC-MS/MS					86:95	HPLC-MS/MS	86:95	HPLC-MS/MS	86:95	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	0	13	theme	components	132:141	arg1	analysis					111:118	quantitative analysis	98:118	quantitative analysis of multiple components by single marker and chemometric methods	98:182	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	1	14	theme	Makino	220:225	arg1	medicine					257:264	a widely used herbal medicine	236:264	a widely used herbal medicine	236:264	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	1	14	theme	Makino	220:225	arg1	rhizome					189:195	The rhizome	185:195	The rhizome of Dioscorea nipponica Makino (RDN)	185:231	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	7	15	theme	chemical	1472:1479	arg1	components					1481:1490	chemical components	1472:1490	chemical components	1472:1490	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	0	16	theme	single	146:151	arg1	marker					153:158	single marker	146:158	single marker	146:158	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	3	17	theme	quantitative	645:656	arg1	analysis					658:665	quantitative analysis	645:665	quantitative analysis of multiple components by single marker (QAMS)	645:712	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	6	18	theme	RAW264.7	1285:1292	arg1	cells					1294:1298	LPS-induced RAW264.7 cells	1273:1298	LPS-induced RAW264.7 cells	1273:1298	The anti-inflammatory effects of RDN samples were then evaluated by inhibition of NO production in LPS-induced RAW264.7 cells.					
36371964	5	19	theme	RND	1122:1124	arg1	samples					1126:1132	RND samples	1122:1132	RND samples using dioscin as the reference analyte	1122:1171	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	3	20	theme	single	693:698	arg1	QAMS					708:711	single marker (QAMS)	693:712	single marker (QAMS)	693:712	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	5	21	theme	steroidal	976:984	arg1	pseudoprotodioscin					1057:1074	pseudoprotodioscin	1057:1074	pseudoprotodioscin	1057:1074	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	21	theme	steroidal	976:984	arg1	gracillin					1109:1117	gracillin	1109:1117	gracillin	1109:1117	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	21	theme	steroidal	976:984	arg1	protogracillin					1020:1033	protogracillin	1020:1033	protogracillin	1020:1033	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	21	theme	steroidal	976:984	arg1	pseudoprogracillin					1077:1094	pseudoprogracillin	1077:1094	pseudoprogracillin	1077:1094	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	21	theme	steroidal	976:984	arg1	saponins					986:993	seven bioactive steroidal saponins	960:993	seven bioactive steroidal saponins	960:993	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	21	theme	steroidal	976:984	arg1	dioscin					1097:1103	dioscin	1097:1103	dioscin	1097:1103	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	21	theme	steroidal	976:984	arg1	protodioscin					1043:1054	methyl protodioscin	1036:1054	methyl protodioscin	1036:1054	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	21	theme	steroidal	976:984	arg1	protodioscin					1006:1017	protodioscin	1006:1017	protodioscin	1006:1017	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	9	22	theme	maximal	1880:1886	arg1	concentration					1899:1911	the half maximal inhibitory concentration	1871:1911	the half maximal inhibitory concentration	1871:1911	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	9	22	theme	maximal	1880:1886	arg1	values					1863:1868	IC50 values	1858:1868	IC50 values (the half maximal inhibitory concentration)	1858:1912	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	3	23	theme	components	679:688	arg1	analysis					658:665	quantitative analysis	645:665	quantitative analysis of multiple components by single marker (QAMS)	645:712	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	3	23	theme	components	679:688	arg1	HPLC-Q/TOF-MS					629:641	HPLC-Q/TOF-MS	629:641	HPLC-Q/TOF-MS	629:641	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	3	23	theme	components	679:688	arg1	methods					730:736	chemometric methods	718:736	chemometric methods	718:736	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	3	23	theme	components	679:688	arg1	spectrometry					615:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	545:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS)	545:642	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	4	24	theme	HPLC-Q/TOF-MS	751:763	arg1	method					765:770	an HPLC-Q/TOF-MS method	748:770	an HPLC-Q/TOF-MS method	748:770	Firstly, an HPLC-Q/TOF-MS method was employed for identification of bioactive steroidal saponins in RND, and a total of twelve steroid saponins were identified.					
36371964	0	25	from	Identification	0:13	arg1	Makino					70:75	Makino	70:75	Makino	70:75	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	0	26	theme	quantitative	98:109	arg1	HPLC-MS/MS					86:95	HPLC-MS/MS	86:95	HPLC-MS/MS	86:95	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	0	26	theme	quantitative	98:109	arg1	analysis					111:118	quantitative analysis	98:118	quantitative analysis of multiple components by single marker and chemometric methods	98:182	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	5	27	theme	reference	1155:1163	arg1	dioscin					1140:1146	dioscin	1140:1146	dioscin	1140:1146	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	27	theme	reference	1155:1163	arg1	analyte					1165:1171	the reference analyte	1151:1171	the reference analyte	1151:1171	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	11	28	theme	identification	2147:2160	arg1	activity					2127:2134	the anti-inflammatory activity	2105:2134	the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN	2105:2203	The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.					
36371964	5	29	theme	methyl	1036:1041	arg1	protodioscin					1043:1054	methyl protodioscin	1036:1054	methyl protodioscin	1036:1054	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	4	30	theme	steroidal	817:825	arg1	saponins					827:834	bioactive steroidal saponins	807:834	bioactive steroidal saponins in RND	807:841	Firstly, an HPLC-Q/TOF-MS method was employed for identification of bioactive steroidal saponins in RND, and a total of twelve steroid saponins were identified.					
36371964	0	31	theme	anti-inflammatory	18:34	arg1	components					36:45	anti-inflammatory components	18:45	anti-inflammatory components in Dioscorea nipponica Makino	18:75	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	1	32	theme	significant	277:287	arg1	activities					307:316	significant anti-inflammatory activities	277:316	significant anti-inflammatory activities	277:316	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	3	33	theme	liquid	562:567	arg1	HPLC-Q/TOF-MS					629:641	HPLC-Q/TOF-MS	629:641	HPLC-Q/TOF-MS	629:641	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	3	33	theme	liquid	562:567	arg1	spectrometry					615:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	545:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS)	545:642	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	4	34	theme	steroid	866:872	arg1	saponins					874:881	twelve steroid saponins	859:881	twelve steroid saponins	859:881	Firstly, an HPLC-Q/TOF-MS method was employed for identification of bioactive steroidal saponins in RND, and a total of twelve steroid saponins were identified.					
36371964	2	35	theme	RDN	438:440	arg1	activity					426:433	the anti-inflammatory activity	404:433	the anti-inflammatory activity of RDN	404:440	However, the bioactive compositions responsible for the anti-inflammatory activity of RDN are still unknown.					
36371964	7	36	theme	least	1386:1390	arg1	squares					1392:1398	partial least squares	1378:1398	partial least squares regression (PLSR)	1378:1416	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	8	37	theme	anti-inflammatory	1688:1704	arg1	protodioscin					1625:1636	protodioscin	1625:1636	protodioscin	1625:1636	The results indicated that protodioscin, dioscin and gracillin were selected as the major anti-inflammatory compounds in RND.					
36371964	8	37	theme	anti-inflammatory	1688:1704	arg1	compounds					1706:1714	the major anti-inflammatory compounds	1678:1714	the major anti-inflammatory compounds in RND	1678:1721	The results indicated that protodioscin, dioscin and gracillin were selected as the major anti-inflammatory compounds in RND.					
36371964	8	37	theme	anti-inflammatory	1688:1704	arg1	gracillin					1651:1659	gracillin	1651:1659	gracillin	1651:1659	The results indicated that protodioscin, dioscin and gracillin were selected as the major anti-inflammatory compounds in RND.					
36371964	8	37	theme	anti-inflammatory	1688:1704	arg1	dioscin					1639:1645	dioscin	1639:1645	dioscin	1639:1645	The results indicated that protodioscin, dioscin and gracillin were selected as the major anti-inflammatory compounds in RND.					
36371964	1	38	theme	various	321:327	arg1	diseases					342:349	various inflammatory diseases	321:349	various inflammatory diseases	321:349	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	9	39	theme	verification	1736:1747	arg1	experiments					1749:1759	The further verification experiments	1724:1759	The further verification experiments	1724:1759	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	10	40	theme	IL-1β	2017:2021	arg1	levels					2000:2005	the levels	1996:2005	the levels of TNF-α, IL-1β, and IL-6 in LPS-induced RAW264.7 cells	1996:2061	They also significantly reduced the levels of TNF-α, IL-1β, and IL-6 in LPS-induced RAW264.7 cells.					
36371964	3	41	theme	anti-inflammatory	494:510	arg1	compounds					522:530	the anti-inflammatory bioactive compounds	490:530	the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods	490:736	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	11	42	theme	RND	2139:2141	arg1	activity					2127:2134	the anti-inflammatory activity	2105:2134	the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN	2105:2203	The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.					
36371964	7	43	theme	correlation	1353:1363	arg1	analysis					1365:1372	Pearson correlation analysis	1345:1372	Pearson correlation analysis	1345:1372	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	3	44	theme	chemometric	718:728	arg1	methods					730:736	chemometric methods	718:736	chemometric methods	718:736	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	3	45	theme	high	545:548	arg1	HPLC-Q/TOF-MS					629:641	HPLC-Q/TOF-MS	629:641	HPLC-Q/TOF-MS	629:641	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	3	45	theme	high	545:548	arg1	spectrometry					615:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	545:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS)	545:642	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	2	46	theme	bioactive	365:373	arg1	unknown					452:458	unknown	452:458	unknown	452:458	However, the bioactive compositions responsible for the anti-inflammatory activity of RDN are still unknown.					
36371964	2	46	theme	bioactive	365:373	arg1	compositions					375:386	the bioactive compositions	361:386	the bioactive compositions responsible for the anti-inflammatory activity of RDN	361:440	However, the bioactive compositions responsible for the anti-inflammatory activity of RDN are still unknown.					
36371964	1	47	theme	used	245:248	arg1	medicine					257:264	a widely used herbal medicine	236:264	a widely used herbal medicine	236:264	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	1	47	theme	used	245:248	arg1	rhizome					189:195	The rhizome	185:195	The rhizome of Dioscorea nipponica Makino (RDN)	185:231	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	10	48	from	levels	2000:2005	arg1	cells					2057:2061	LPS-induced RAW264.7 cells	2036:2061	LPS-induced RAW264.7 cells	2036:2061	They also significantly reduced the levels of TNF-α, IL-1β, and IL-6 in LPS-induced RAW264.7 cells.					
36371964	7	49	theme	RDN	1593:1595	arg1	compounds					1580:1588	the potential anti-inflammatory bioactive compounds	1538:1588	the potential anti-inflammatory bioactive compounds of RDN	1538:1595	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	3	50	theme	time-of-flight	595:608	arg1	HPLC-Q/TOF-MS					629:641	HPLC-Q/TOF-MS	629:641	HPLC-Q/TOF-MS	629:641	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	3	50	theme	time-of-flight	595:608	arg1	spectrometry					615:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	545:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS)	545:642	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	7	51	theme	potential	1542:1550	arg1	compounds					1580:1588	the potential anti-inflammatory bioactive compounds	1538:1588	the potential anti-inflammatory bioactive compounds of RDN	1538:1595	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	1	52	contain	has	273:275	arg1	medicine					257:264	a widely used herbal medicine	236:264	a widely used herbal medicine	236:264	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	1	52	contain	has	273:275	arg2	activities					307:316	significant anti-inflammatory activities	277:316	significant anti-inflammatory activities	277:316	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	1	52	contain	has	273:275	arg1	rhizome					189:195	The rhizome	185:195	The rhizome of Dioscorea nipponica Makino (RDN)	185:231	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	5	53	theme	QAMS	906:909	arg1	method					911:916	QAMS method	906:916	QAMS method	906:916	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	7	54	theme	anti-inflammatory	1552:1568	arg1	compounds					1580:1588	the potential anti-inflammatory bioactive compounds	1538:1588	the potential anti-inflammatory bioactive compounds of RDN	1538:1595	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	9	55	theme	great	1819:1823	arg1	inhibition					1825:1834	great inhibition	1819:1834	great inhibition on NO production	1819:1851	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	10	56	theme	RAW264.7	2048:2055	arg1	cells					2057:2061	LPS-induced RAW264.7 cells	2036:2061	LPS-induced RAW264.7 cells	2036:2061	They also significantly reduced the levels of TNF-α, IL-1β, and IL-6 in LPS-induced RAW264.7 cells.					
36371964	8	57	from	compounds	1706:1714	arg1	RND					1719:1721	RND	1719:1721	RND	1719:1721	The results indicated that protodioscin, dioscin and gracillin were selected as the major anti-inflammatory compounds in RND.					
36371964	11	58	theme	anti-inflammatory	2169:2185	arg1	components					2187:2196	the anti-inflammatory components	2165:2196	the anti-inflammatory components in RDN	2165:2203	The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.					
36371964	9	59	from	inhibition	1825:1834	arg1	production					1842:1851	NO production	1839:1851	NO production	1839:1851	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	1	60	theme	nipponica	210:218	arg1	Makino					220:225	Dioscorea nipponica Makino	200:225	Dioscorea nipponica Makino (RDN)	200:231	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	1	60	theme	nipponica	210:218	arg1	RDN					228:230	RDN	228:230	RDN	228:230	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	6	61	theme	samples	1211:1217	arg1	effects					1196:1202	The anti-inflammatory effects	1174:1202	The anti-inflammatory effects of RDN samples	1174:1217	The anti-inflammatory effects of RDN samples were then evaluated by inhibition of NO production in LPS-induced RAW264.7 cells.					
36371964	0	62	theme	marker	153:158	arg1	methods					176:182	single marker and chemometric methods	146:182	single marker and chemometric methods	146:182	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	9	63	theme	IC50	1858:1861	arg1	concentration					1899:1911	the half maximal inhibitory concentration	1871:1911	the half maximal inhibitory concentration	1871:1911	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	9	63	theme	IC50	1858:1861	arg1	values					1863:1868	IC50 values	1858:1868	IC50 values (the half maximal inhibitory concentration)	1858:1912	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	0	64	theme	chemometric	164:174	arg1	methods					176:182	single marker and chemometric methods	146:182	single marker and chemometric methods	146:182	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	11	65	theme	present	2068:2074	arg1	study					2076:2080	The present study	2064:2080	The present study	2064:2080	The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.					
36371964	11	66	theme	anti-inflammatory	2109:2125	arg1	activity					2127:2134	the anti-inflammatory activity	2105:2134	the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN	2105:2203	The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.					
36371964	4	67	from	RND	839:841	arg1	identification					789:802	identification	789:802	identification of bioactive steroidal saponins in RND	789:841	Firstly, an HPLC-Q/TOF-MS method was employed for identification of bioactive steroidal saponins in RND, and a total of twelve steroid saponins were identified.					
36371964	5	68	theme	bioactive	966:974	arg1	pseudoprotodioscin					1057:1074	pseudoprotodioscin	1057:1074	pseudoprotodioscin	1057:1074	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	68	theme	bioactive	966:974	arg1	gracillin					1109:1117	gracillin	1109:1117	gracillin	1109:1117	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	68	theme	bioactive	966:974	arg1	protogracillin					1020:1033	protogracillin	1020:1033	protogracillin	1020:1033	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	68	theme	bioactive	966:974	arg1	pseudoprogracillin					1077:1094	pseudoprogracillin	1077:1094	pseudoprogracillin	1077:1094	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	68	theme	bioactive	966:974	arg1	saponins					986:993	seven bioactive steroidal saponins	960:993	seven bioactive steroidal saponins	960:993	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	68	theme	bioactive	966:974	arg1	dioscin					1097:1103	dioscin	1097:1103	dioscin	1097:1103	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	68	theme	bioactive	966:974	arg1	protodioscin					1043:1054	methyl protodioscin	1036:1054	methyl protodioscin	1036:1054	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	5	68	theme	bioactive	966:974	arg1	protodioscin					1006:1017	protodioscin	1006:1017	protodioscin	1006:1017	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	6	69	theme	LPS-induced	1273:1283	arg1	cells					1294:1298	LPS-induced RAW264.7 cells	1273:1298	LPS-induced RAW264.7 cells	1273:1298	The anti-inflammatory effects of RDN samples were then evaluated by inhibition of NO production in LPS-induced RAW264.7 cells.					
36371964	3	70	theme	multiple	670:677	arg1	components					679:688	multiple components	670:688	multiple components	670:688	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	9	71	theme	inhibitory	1888:1897	arg1	concentration					1899:1911	the half maximal inhibitory concentration	1871:1911	the half maximal inhibitory concentration	1871:1911	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	9	71	theme	inhibitory	1888:1897	arg1	values					1863:1868	IC50 values	1858:1868	IC50 values (the half maximal inhibitory concentration)	1858:1912	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	0	72	from	components	36:45	arg1	Makino					70:75	Makino	70:75	Makino	70:75	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	6	73	theme	production	1259:1268	arg1	inhibition					1242:1251	inhibition	1242:1251	inhibition of NO production in LPS-induced RAW264.7 cells	1242:1298	The anti-inflammatory effects of RDN samples were then evaluated by inhibition of NO production in LPS-induced RAW264.7 cells.					
36371964	11	74	from	RDN	2201:2203	arg1	identification					2147:2160	identification	2147:2160	identification	2147:2160	The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.					
36371964	11	74	from	RDN	2201:2203	arg1	RND					2139:2141	RND	2139:2141	RND	2139:2141	The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.					
36371964	5	75	from	contents	948:955	arg1	samples					1126:1132	RND samples	1122:1132	RND samples using dioscin as the reference analyte	1122:1171	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	7	76	theme	anti-inflammatory	1496:1512	arg1	activities					1514:1523	anti-inflammatory activities	1496:1523	anti-inflammatory activities	1496:1523	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	9	77	theme	0.712 μM	1917:1924	arg1	concentration					1899:1911	the half maximal inhibitory concentration	1871:1911	the half maximal inhibitory concentration	1871:1911	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	9	77	theme	0.712 μM	1917:1924	arg1	values					1863:1868	IC50 values	1858:1868	IC50 values (the half maximal inhibitory concentration)	1858:1912	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	0	78	from	Makino	70:75	arg1	Identification					0:13	Identification	0:13	Identification of anti-inflammatory components in Dioscorea nipponica Makino	0:75	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	4	79	theme	saponins	827:834	arg1	identification					789:802	identification	789:802	identification of bioactive steroidal saponins in RND	789:841	Firstly, an HPLC-Q/TOF-MS method was employed for identification of bioactive steroidal saponins in RND, and a total of twelve steroid saponins were identified.					
36371964	6	80	from	inhibition	1242:1251	arg1	cells					1294:1298	LPS-induced RAW264.7 cells	1273:1298	LPS-induced RAW264.7 cells	1273:1298	The anti-inflammatory effects of RDN samples were then evaluated by inhibition of NO production in LPS-induced RAW264.7 cells.					
36371964	6	81	theme	anti-inflammatory	1178:1194	arg1	effects					1196:1202	The anti-inflammatory effects	1174:1202	The anti-inflammatory effects of RDN samples	1174:1217	The anti-inflammatory effects of RDN samples were then evaluated by inhibition of NO production in LPS-induced RAW264.7 cells.					
36371964	0	82	theme	multiple	123:130	arg1	components					132:141	multiple components	123:141	multiple components	123:141	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	4	83	from	saponins	827:834	arg1	RND					839:841	RND	839:841	RND	839:841	Firstly, an HPLC-Q/TOF-MS method was employed for identification of bioactive steroidal saponins in RND, and a total of twelve steroid saponins were identified.					
36371964	9	84	theme	0.469 μM	1927:1934	arg1	concentration					1899:1911	the half maximal inhibitory concentration	1871:1911	the half maximal inhibitory concentration	1871:1911	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	9	84	theme	0.469 μM	1927:1934	arg1	values					1863:1868	IC50 values	1858:1868	IC50 values (the half maximal inhibitory concentration)	1858:1912	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	2	85	theme	anti-inflammatory	408:424	arg1	activity					426:433	the anti-inflammatory activity	404:433	the anti-inflammatory activity of RDN	404:440	However, the bioactive compositions responsible for the anti-inflammatory activity of RDN are still unknown.					
36371964	5	86	theme	saponins	986:993	arg1	contents					948:955	the contents	944:955	the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte	944:1171	Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte.					
36371964	9	87	theme	0.815 μM	1940:1947	arg1	concentration					1899:1911	the half maximal inhibitory concentration	1871:1911	the half maximal inhibitory concentration	1871:1911	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	9	87	theme	0.815 μM	1940:1947	arg1	values					1863:1868	IC50 values	1858:1868	IC50 values (the half maximal inhibitory concentration)	1858:1912	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	10	88	theme	TNF-α	2010:2014	arg1	levels					2000:2005	the levels	1996:2005	the levels of TNF-α, IL-1β, and IL-6 in LPS-induced RAW264.7 cells	1996:2061	They also significantly reduced the levels of TNF-α, IL-1β, and IL-6 in LPS-induced RAW264.7 cells.					
36371964	0	89	theme	components	36:45	arg1	Identification					0:13	Identification	0:13	Identification of anti-inflammatory components in Dioscorea nipponica Makino	0:75	Identification of anti-inflammatory components in Dioscorea nipponica Makino based on HPLC-MS/MS, quantitative analysis of multiple components by single marker and chemometric methods.					
36371964	4	90	from	identification	789:802	arg1	RND					839:841	RND	839:841	RND	839:841	Firstly, an HPLC-Q/TOF-MS method was employed for identification of bioactive steroidal saponins in RND, and a total of twelve steroid saponins were identified.					
36371964	7	91	theme	squares	1392:1398	arg1	PLSR					1412:1415	PLSR	1412:1415	PLSR	1412:1415	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	7	91	theme	squares	1392:1398	arg1	regression					1400:1409	partial least squares regression	1378:1409	partial least squares regression (PLSR)	1378:1416	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	8	92	theme	major	1682:1686	arg1	protodioscin					1625:1636	protodioscin	1625:1636	protodioscin	1625:1636	The results indicated that protodioscin, dioscin and gracillin were selected as the major anti-inflammatory compounds in RND.					
36371964	8	92	theme	major	1682:1686	arg1	compounds					1706:1714	the major anti-inflammatory compounds	1678:1714	the major anti-inflammatory compounds in RND	1678:1721	The results indicated that protodioscin, dioscin and gracillin were selected as the major anti-inflammatory compounds in RND.					
36371964	8	92	theme	major	1682:1686	arg1	gracillin					1651:1659	gracillin	1651:1659	gracillin	1651:1659	The results indicated that protodioscin, dioscin and gracillin were selected as the major anti-inflammatory compounds in RND.					
36371964	8	92	theme	major	1682:1686	arg1	dioscin					1639:1645	dioscin	1639:1645	dioscin	1639:1645	The results indicated that protodioscin, dioscin and gracillin were selected as the major anti-inflammatory compounds in RND.					
36371964	4	93	theme	bioactive	807:815	arg1	saponins					827:834	bioactive steroidal saponins	807:834	bioactive steroidal saponins in RND	807:841	Firstly, an HPLC-Q/TOF-MS method was employed for identification of bioactive steroidal saponins in RND, and a total of twelve steroid saponins were identified.					
36371964	2	94	theme	responsible	388:398	arg1	unknown					452:458	unknown	452:458	unknown	452:458	However, the bioactive compositions responsible for the anti-inflammatory activity of RDN are still unknown.					
36371964	2	94	theme	responsible	388:398	arg1	compositions					375:386	the bioactive compositions	361:386	the bioactive compositions responsible for the anti-inflammatory activity of RDN	361:440	However, the bioactive compositions responsible for the anti-inflammatory activity of RDN are still unknown.					
36371964	1	95	theme	anti-inflammatory	289:305	arg1	activities					307:316	significant anti-inflammatory activities	277:316	significant anti-inflammatory activities	277:316	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	7	96	theme	partial	1378:1384	arg1	squares					1392:1398	partial least squares	1378:1398	partial least squares regression (PLSR)	1378:1416	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	3	97	theme	marker	700:705	arg1	QAMS					708:711	single marker (QAMS)	693:712	single marker (QAMS)	693:712	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	9	98	theme	further	1728:1734	arg1	experiments					1749:1759	The further verification experiments	1724:1759	The further verification experiments	1724:1759	The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively.					
36371964	6	99	theme	RDN	1207:1209	arg1	samples					1211:1217	RDN samples	1207:1217	RDN samples	1207:1217	The anti-inflammatory effects of RDN samples were then evaluated by inhibition of NO production in LPS-induced RAW264.7 cells.					
36371964	4	100	theme	saponins	874:881	arg1	total					850:854	a total	848:854	a total of twelve steroid saponins	848:881	Firstly, an HPLC-Q/TOF-MS method was employed for identification of bioactive steroidal saponins in RND, and a total of twelve steroid saponins were identified.					
36371964	1	101	theme	inflammatory	329:340	arg1	diseases					342:349	various inflammatory diseases	321:349	various inflammatory diseases	321:349	The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases.					
36371964	7	102	theme	Pearson	1345:1351	arg1	correlation					1353:1363	Pearson correlation	1345:1363	Pearson correlation analysis	1345:1372	Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN.					
36371964	3	103	theme	bioactive	512:520	arg1	compounds					522:530	the anti-inflammatory bioactive compounds	490:530	the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods	490:736	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	11	104	from	components	2187:2196	arg1	RDN					2201:2203	RDN	2201:2203	RDN	2201:2203	The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.					
36371964	10	105	theme	LPS-induced	2036:2046	arg1	cells					2057:2061	LPS-induced RAW264.7 cells	2036:2061	LPS-induced RAW264.7 cells	2036:2061	They also significantly reduced the levels of TNF-α, IL-1β, and IL-6 in LPS-induced RAW264.7 cells.					
36371964	3	106	theme	performance	550:560	arg1	HPLC-Q/TOF-MS					629:641	HPLC-Q/TOF-MS	629:641	HPLC-Q/TOF-MS	629:641	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
36371964	3	106	theme	performance	550:560	arg1	spectrometry					615:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	545:626	high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS)	545:642	This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods.					
37087226	5	0	theme	chitosan	1032:1039	arg1	coating					1041:1047	chitosan coating	1032:1047	chitosan coating	1032:1047	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	6	1	theme	primary	1145:1151	arg1	Pseudomonas					1216:1226	Pseudomonas	1216:1226	Pseudomonas	1216:1226	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	1	theme	primary	1145:1151	arg1	Brochothrix					1173:1183	Brochothrix	1173:1183	Brochothrix	1173:1183	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	1	theme	primary	1145:1151	arg1	organisms					1162:1170	the four primary spoilage organisms	1136:1170	the four primary spoilage organisms	1136:1170	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	1	theme	primary	1145:1151	arg1	Acinetobacter					1197:1209	Acinetobacter	1197:1209	Acinetobacter	1197:1209	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	2	theme	spoilage	1153:1160	arg1	Pseudomonas					1216:1226	Pseudomonas	1216:1226	Pseudomonas	1216:1226	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	2	theme	spoilage	1153:1160	arg1	Brochothrix					1173:1183	Brochothrix	1173:1183	Brochothrix	1173:1183	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	2	theme	spoilage	1153:1160	arg1	organisms					1162:1170	the four primary spoilage organisms	1136:1170	the four primary spoilage organisms	1136:1170	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	2	theme	spoilage	1153:1160	arg1	Acinetobacter					1197:1209	Acinetobacter	1197:1209	Acinetobacter	1197:1209	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	2	3	theme	preserved	538:546	arg1	treatments					548:557	the three preserved treatments	528:557	the three preserved treatments	528:557	The findings revealed that the three preserved treatments significantly increased tenderness from days 1 to 3.					
37087226	3	4	from	days	650:653	arg1	decrease					636:643	a substantial decrease	622:643	a substantial decrease from days 6 to 12	622:661	There was a substantial decrease from days 6 to 12 compared to the control, but no significant differences were observed on day 15.					
37087226	1	5	from	compounds	395:403	arg1	meat					425:428	braised duck leg meat	408:428	braised duck leg meat	408:428	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	4	6	theme	TBARS	810:814	arg1	values					816:821	TBARS values	810:821	TBARS values	810:821	Compared with the control, the three preserved treatments reduced TBARS values by 25.8%-78.6% (from days 6 to 12) and total sulfhydryl concentrations by 24.1%-75.7% (from days 3 to 9).					
37087226	1	7	theme	braised	408:414	arg1	meat					425:428	braised duck leg meat	408:428	braised duck leg meat	408:428	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	7	8	theme	most	1394:1397	arg1	compounds					1415:1423	the most volatile flavor compounds	1390:1423	the most volatile flavor compounds (38 compounds)	1390:1438	The combination treatment produced the most volatile flavor compounds (38 compounds) at the end of storage (15 days).					
37087226	7	8	theme	most	1394:1397	arg1	compounds					1429:1437	38 compounds	1426:1437	38 compounds	1426:1437	The combination treatment produced the most volatile flavor compounds (38 compounds) at the end of storage (15 days).					
37087226	9	9	theme	thermal	1650:1656	arg1	methods					1702:1708	synergistic methods	1690:1708	synergistic methods	1690:1708	Therefore, chitosan coating and thermal treatment could be developed as synergistic methods to preserve braised duck meat.					
37087226	9	9	theme	thermal	1650:1656	arg1	coating					1638:1644	chitosan coating	1629:1644	chitosan coating	1629:1644	Therefore, chitosan coating and thermal treatment could be developed as synergistic methods to preserve braised duck meat.					
37087226	9	9	theme	thermal	1650:1656	arg1	treatment					1658:1666	thermal treatment	1650:1666	thermal treatment	1650:1666	Therefore, chitosan coating and thermal treatment could be developed as synergistic methods to preserve braised duck meat.					
37087226	5	10	theme	combination	933:943	arg1	treatment					945:953	The combination treatment	929:953	The combination treatment	929:953	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	1	11	theme	lipid	325:329	arg1	oxidation					343:351	lipid and protein oxidation	325:351	oxidation	343:351	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	1	12	theme	duck	416:419	arg1	meat					425:428	braised duck leg meat	408:428	braised duck leg meat	408:428	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	8	13	theme	peak	1594:1597	arg1	area					1599:1602	the average relative peak area	1573:1602	the average relative peak area	1573:1602	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	0	14	theme	bacterial	91:99	arg1	diversity					101:109	bacterial diversity	91:109	bacterial diversity	91:109	Effects of chitosan coating combined with thermal treatment on physicochemical properties, bacterial diversity and volatile flavor of braised duck meat during refrigerated storage.					
37087226	5	15	theme	lowest	963:968	arg1	TVC					1002:1004	TVC	1002:1004	TVC	1002:1004	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	5	15	theme	lowest	963:968	arg1	values					970:975	the lowest values	959:975	the lowest values (carbonyl concentration, TVC, and TEC)	959:1014	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	5	15	theme	lowest	963:968	arg1	concentration					987:999	carbonyl concentration	978:999	carbonyl concentration	978:999	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	5	15	theme	lowest	963:968	arg1	TEC					1011:1013	TEC	1011:1013	TEC	1011:1013	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	9	16	theme	duck	1730:1733	arg1	meat					1735:1738	braised duck meat	1722:1738	braised duck meat	1722:1738	Therefore, chitosan coating and thermal treatment could be developed as synergistic methods to preserve braised duck meat.					
37087226	1	17	theme	leg	421:423	arg1	meat					425:428	braised duck leg meat	408:428	braised duck leg meat	408:428	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	3	18	dep	days	650:653	arg1	to					657:658	to	657:658	to	657:658	There was a substantial decrease from days 6 to 12 compared to the control, but no significant differences were observed on day 15.					
37087226	0	19	theme	volatile	115:122	arg1	flavor					124:129	volatile flavor	115:129	volatile flavor	115:129	Effects of chitosan coating combined with thermal treatment on physicochemical properties, bacterial diversity and volatile flavor of braised duck meat during refrigerated storage.					
37087226	1	20	theme	protein	335:341	arg1	oxidation					343:351	lipid and protein oxidation	325:351	oxidation	343:351	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	4	21	theme	preserved	781:789	arg1	treatments					791:800	the three preserved treatments	771:800	the three preserved treatments	771:800	Compared with the control, the three preserved treatments reduced TBARS values by 25.8%-78.6% (from days 6 to 12) and total sulfhydryl concentrations by 24.1%-75.7% (from days 3 to 9).					
37087226	8	22	theme	relative	1585:1592	arg1	area					1599:1602	the average relative peak area	1573:1602	the average relative peak area	1573:1602	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	9	23	theme	chitosan	1629:1636	arg1	methods					1702:1708	synergistic methods	1690:1708	synergistic methods	1690:1708	Therefore, chitosan coating and thermal treatment could be developed as synergistic methods to preserve braised duck meat.					
37087226	9	23	theme	chitosan	1629:1636	arg1	coating					1638:1644	chitosan coating	1629:1644	chitosan coating	1629:1644	Therefore, chitosan coating and thermal treatment could be developed as synergistic methods to preserve braised duck meat.					
37087226	9	23	theme	chitosan	1629:1636	arg1	treatment					1658:1666	thermal treatment	1650:1666	thermal treatment	1650:1666	Therefore, chitosan coating and thermal treatment could be developed as synergistic methods to preserve braised duck meat.					
37087226	1	24	from	tenderness	313:322	arg1	meat					425:428	braised duck leg meat	408:428	braised duck leg meat	408:428	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	5	25	contain	had	955:957	arg1	treatment					945:953	The combination treatment	929:953	The combination treatment	929:953	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	5	25	contain	had	955:957	arg2	TVC					1002:1004	TVC	1002:1004	TVC	1002:1004	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	5	25	contain	had	955:957	arg2	concentration					987:999	carbonyl concentration	978:999	carbonyl concentration	978:999	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	5	25	contain	had	955:957	arg2	values					970:975	the lowest values	959:975	the lowest values (carbonyl concentration, TVC, and TEC)	959:1014	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	5	25	contain	had	955:957	arg2	TEC					1011:1013	TEC	1011:1013	TEC	1011:1013	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	4	26	theme	total	862:866	arg1	concentrations					879:892	total sulfhydryl concentrations	862:892	total sulfhydryl concentrations	862:892	Compared with the control, the three preserved treatments reduced TBARS values by 25.8%-78.6% (from days 6 to 12) and total sulfhydryl concentrations by 24.1%-75.7% (from days 3 to 9).					
37087226	1	27	from	oxidation	343:351	arg1	meat					425:428	braised duck leg meat	408:428	braised duck leg meat	408:428	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	5	28	theme	carbonyl	978:985	arg1	values					970:975	the lowest values	959:975	the lowest values (carbonyl concentration, TVC, and TEC)	959:1014	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	5	28	theme	carbonyl	978:985	arg1	concentration					987:999	carbonyl concentration	978:999	carbonyl concentration	978:999	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	4	29	dep	days	915:918	arg1	to					922:923	to	922:923	to	922:923	Compared with the control, the three preserved treatments reduced TBARS values by 25.8%-78.6% (from days 6 to 12) and total sulfhydryl concentrations by 24.1%-75.7% (from days 3 to 9).					
37087226	1	30	theme	vacuum	436:441	arg1	packaging					443:451	vacuum packaging	436:451	vacuum packaging	436:451	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	1	31	dep	storage	473:479	arg1	4 °C					482:485	4 °C	482:485	4 °C for 15 days	482:497	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	1	32	theme	current	185:191	arg1	study					193:197	The current study	181:197	The current study	181:197	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	2	33	dep	days	599:602	arg1	to					606:607	to	606:607	to	606:607	The findings revealed that the three preserved treatments significantly increased tenderness from days 1 to 3.					
37087226	3	34	theme	significant	695:705	arg1	differences					707:717	no significant differences	692:717	no significant differences	692:717	There was a substantial decrease from days 6 to 12 compared to the control, but no significant differences were observed on day 15.					
37087226	6	35	from	%	1245:1245	arg1	control					1268:1274	control	1268:1274	control	1268:1274	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	35	from	%	1245:1245	arg1	coating					1286:1292	chitosan coating	1277:1292	chitosan coating	1277:1292	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	35	from	%	1245:1245	arg1	treatment					1303:1311	thermal treatment	1295:1311	thermal treatment	1295:1311	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	35	from	%	1245:1245	arg1	treatment					1330:1338	combination treatment	1318:1338	combination treatment	1318:1338	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	36	theme	combination	1318:1328	arg1	treatment					1330:1338	combination treatment	1318:1338	combination treatment	1318:1338	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	0	37	theme	coating	20:26	arg1	Effects					0:6	Effects	0:6	Effects of chitosan coating	0:26	Effects of chitosan coating combined with thermal treatment on physicochemical properties, bacterial diversity and volatile flavor of braised duck meat during refrigerated storage.					
37087226	1	38	theme	thermal	273:279	arg1	treatment					281:289	thermal treatment	273:289	thermal treatment (85 °C for 30 min)	273:308	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	3	39	located	observed	724:731	arg2	differences					707:717	no significant differences	692:717	no significant differences	692:717	There was a substantial decrease from days 6 to 12 compared to the control, but no significant differences were observed on day 15.					
37087226	3	39	located	observed	724:731	arg1	day					736:738	day 15	736:741	day 15	736:741	There was a substantial decrease from days 6 to 12 compared to the control, but no significant differences were observed on day 15.					
37087226	8	40	theme	average	1577:1583	arg1	area					1599:1602	the average relative peak area	1573:1602	the average relative peak area	1573:1602	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	0	41	theme	chitosan	11:18	arg1	coating					20:26	chitosan coating	11:26	chitosan coating	11:26	Effects of chitosan coating combined with thermal treatment on physicochemical properties, bacterial diversity and volatile flavor of braised duck meat during refrigerated storage.					
37087226	4	42	from	days	915:918	arg1	%					907:907	24.1%-75.7%	897:907	24.1%-75.7% (from days 3 to 9)	897:926	Compared with the control, the three preserved treatments reduced TBARS values by 25.8%-78.6% (from days 6 to 12) and total sulfhydryl concentrations by 24.1%-75.7% (from days 3 to 9).					
37087226	1	43	theme	bacterial	354:362	arg1	diversity					364:372	bacterial diversity	354:372	bacterial diversity	354:372	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	9	44	theme	synergistic	1690:1700	arg1	methods					1702:1708	synergistic methods	1690:1708	synergistic methods	1690:1708	Therefore, chitosan coating and thermal treatment could be developed as synergistic methods to preserve braised duck meat.					
37087226	9	44	theme	synergistic	1690:1700	arg1	coating					1638:1644	chitosan coating	1629:1644	chitosan coating	1629:1644	Therefore, chitosan coating and thermal treatment could be developed as synergistic methods to preserve braised duck meat.					
37087226	9	44	theme	synergistic	1690:1700	arg1	treatment					1658:1666	thermal treatment	1650:1666	thermal treatment	1650:1666	Therefore, chitosan coating and thermal treatment could be developed as synergistic methods to preserve braised duck meat.					
37087226	0	45	theme	duck	142:145	arg1	meat					147:150	braised duck meat	134:150	braised duck meat during refrigerated storage	134:178	Effects of chitosan coating combined with thermal treatment on physicochemical properties, bacterial diversity and volatile flavor of braised duck meat during refrigerated storage.					
37087226	6	46	from	%	1252:1252	arg1	control					1268:1274	control	1268:1274	control	1268:1274	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	46	from	%	1252:1252	arg1	coating					1286:1292	chitosan coating	1277:1292	chitosan coating	1277:1292	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	46	from	%	1252:1252	arg1	treatment					1303:1311	thermal treatment	1295:1311	thermal treatment	1295:1311	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	46	from	%	1252:1252	arg1	treatment					1330:1338	combination treatment	1318:1338	combination treatment	1318:1338	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	7	47	theme	storage	1454:1460	arg1	end					1447:1449	the end	1443:1449	the end of storage (15 days)	1443:1470	The combination treatment produced the most volatile flavor compounds (38 compounds) at the end of storage (15 days).					
37087226	5	48	theme	thermal	1053:1059	arg1	treatment					1061:1069	thermal treatment	1053:1069	thermal treatment	1053:1069	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	4	49	theme	%	901:901	arg1	%					907:907	24.1%-75.7%	897:907	24.1%-75.7% (from days 3 to 9)	897:926	Compared with the control, the three preserved treatments reduced TBARS values by 25.8%-78.6% (from days 6 to 12) and total sulfhydryl concentrations by 24.1%-75.7% (from days 3 to 9).					
37087226	0	50	theme	braised	134:140	arg1	meat					147:150	braised duck meat	134:150	braised duck meat during refrigerated storage	134:178	Effects of chitosan coating combined with thermal treatment on physicochemical properties, bacterial diversity and volatile flavor of braised duck meat during refrigerated storage.					
37087226	4	51	from	days	844:847	arg1	%					836:836	25.8%-78.6%	826:836	25.8%-78.6% (from days 6 to 12)	826:856	Compared with the control, the three preserved treatments reduced TBARS values by 25.8%-78.6% (from days 6 to 12) and total sulfhydryl concentrations by 24.1%-75.7% (from days 3 to 9).					
37087226	4	51	from	days	844:847	arg1	concentrations					879:892	total sulfhydryl concentrations	862:892	total sulfhydryl concentrations	862:892	Compared with the control, the three preserved treatments reduced TBARS values by 25.8%-78.6% (from days 6 to 12) and total sulfhydryl concentrations by 24.1%-75.7% (from days 3 to 9).					
37087226	4	52	dep	days	844:847	arg1	to					851:852	to	851:852	to	851:852	Compared with the control, the three preserved treatments reduced TBARS values by 25.8%-78.6% (from days 6 to 12) and total sulfhydryl concentrations by 24.1%-75.7% (from days 3 to 9).					
37087226	1	53	theme	refrigerated	460:471	arg1	storage					473:479	refrigerated storage	460:479	refrigerated storage (4 °C for 15 days)	460:498	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	1	54	from	diversity	364:372	arg1	meat					425:428	braised duck leg meat	408:428	braised duck leg meat	408:428	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	6	55	from	%	1238:1238	arg1	control					1268:1274	control	1268:1274	control	1268:1274	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	55	from	%	1238:1238	arg1	coating					1286:1292	chitosan coating	1277:1292	chitosan coating	1277:1292	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	55	from	%	1238:1238	arg1	treatment					1303:1311	thermal treatment	1295:1311	thermal treatment	1295:1311	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	55	from	%	1238:1238	arg1	treatment					1330:1338	combination treatment	1318:1338	combination treatment	1318:1338	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	5	56	dep	coating	1041:1047	arg1	the					1028:1030	the	1028:1030	the	1028:1030	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	6	57	theme	organisms	1162:1170	arg1	proportions					1121:1131	The proportions	1117:1131	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas,	1117:1227	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	57	theme	organisms	1162:1170	arg1	%					1238:1238	74.8%	1234:1238	74.8%	1234:1238	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	0	58	theme	thermal	42:48	arg1	treatment					50:58	thermal treatment	42:58	thermal treatment	42:58	Effects of chitosan coating combined with thermal treatment on physicochemical properties, bacterial diversity and volatile flavor of braised duck meat during refrigerated storage.					
37087226	0	59	theme	meat	147:150	arg1	properties					79:88	physicochemical properties	63:88	physicochemical properties	63:88	Effects of chitosan coating combined with thermal treatment on physicochemical properties, bacterial diversity and volatile flavor of braised duck meat during refrigerated storage.					
37087226	0	59	theme	meat	147:150	arg1	diversity					101:109	bacterial diversity	91:109	bacterial diversity	91:109	Effects of chitosan coating combined with thermal treatment on physicochemical properties, bacterial diversity and volatile flavor of braised duck meat during refrigerated storage.					
37087226	0	59	theme	meat	147:150	arg1	flavor					124:129	volatile flavor	115:129	volatile flavor	115:129	Effects of chitosan coating combined with thermal treatment on physicochemical properties, bacterial diversity and volatile flavor of braised duck meat during refrigerated storage.					
37087226	1	60	theme	volatile	379:386	arg1	compounds					395:403	volatile flavor compounds	379:403	volatile flavor compounds	379:403	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	8	61	theme	volatile	1542:1549	arg1	1-octen-3-ol					1493:1504	1-octen-3-ol	1493:1504	1-octen-3-ol	1493:1504	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	8	61	theme	volatile	1542:1549	arg1	compounds					1558:1566	the main volatile flavor compounds	1533:1566	the main volatile flavor compounds	1533:1566	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	8	61	theme	volatile	1542:1549	arg1	1-nonanal					1482:1490	1-nonanal	1482:1490	1-nonanal	1482:1490	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	8	61	theme	volatile	1542:1549	arg1	Hexanal					1473:1479	Hexanal	1473:1479	Hexanal	1473:1479	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	8	61	theme	volatile	1542:1549	arg1	2					1511:1511	2	1511:1511	2	1511:1511	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	6	62	theme	thermal	1295:1301	arg1	treatment					1303:1311	thermal treatment	1295:1311	thermal treatment	1295:1311	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	5	63	dep	values	970:975	arg1	TVC					1002:1004	TVC	1002:1004	TVC	1002:1004	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	5	63	dep	values	970:975	arg1	TEC					1011:1013	TEC	1011:1013	TEC	1011:1013	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	5	63	dep	values	970:975	arg1	concentration					987:999	carbonyl concentration	978:999	carbonyl concentration	978:999	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	5	63	dep	values	970:975	arg1	values					970:975	the lowest values	959:975	the lowest values (carbonyl concentration, TVC, and TEC)	959:1014	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	1	64	from	impact	219:224	arg1	oxidation					343:351	lipid and protein oxidation	325:351	oxidation	343:351	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	1	64	from	impact	219:224	arg1	compounds					395:403	volatile flavor compounds	379:403	volatile flavor compounds	379:403	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	1	64	from	impact	219:224	arg1	diversity					364:372	bacterial diversity	354:372	bacterial diversity	354:372	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	1	64	from	impact	219:224	arg1	tenderness					313:322	tenderness	313:322	tenderness	313:322	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	1	65	theme	flavor	388:393	arg1	compounds					395:403	volatile flavor compounds	379:403	volatile flavor compounds	379:403	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	8	66	theme	main	1537:1540	arg1	1-octen-3-ol					1493:1504	1-octen-3-ol	1493:1504	1-octen-3-ol	1493:1504	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	8	66	theme	main	1537:1540	arg1	compounds					1558:1566	the main volatile flavor compounds	1533:1566	the main volatile flavor compounds	1533:1566	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	8	66	theme	main	1537:1540	arg1	1-nonanal					1482:1490	1-nonanal	1482:1490	1-nonanal	1482:1490	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	8	66	theme	main	1537:1540	arg1	Hexanal					1473:1479	Hexanal	1473:1479	Hexanal	1473:1479	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	8	66	theme	main	1537:1540	arg1	2					1511:1511	2	1511:1511	2	1511:1511	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	7	67	theme	volatile	1399:1406	arg1	compounds					1415:1423	the most volatile flavor compounds	1390:1423	the most volatile flavor compounds (38 compounds)	1390:1438	The combination treatment produced the most volatile flavor compounds (38 compounds) at the end of storage (15 days).					
37087226	7	67	theme	volatile	1399:1406	arg1	compounds					1429:1437	38 compounds	1426:1437	38 compounds	1426:1437	The combination treatment produced the most volatile flavor compounds (38 compounds) at the end of storage (15 days).					
37087226	0	68	theme	refrigerated	159:170	arg1	storage					172:178	refrigerated storage	159:178	refrigerated storage	159:178	Effects of chitosan coating combined with thermal treatment on physicochemical properties, bacterial diversity and volatile flavor of braised duck meat during refrigerated storage.					
37087226	3	69	theme	substantial	624:634	arg1	decrease					636:643	a substantial decrease	622:643	a substantial decrease from days 6 to 12	622:661	There was a substantial decrease from days 6 to 12 compared to the control, but no significant differences were observed on day 15.					
37087226	6	70	from	%	1263:1263	arg1	control					1268:1274	control	1268:1274	control	1268:1274	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	70	from	%	1263:1263	arg1	coating					1286:1292	chitosan coating	1277:1292	chitosan coating	1277:1292	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	70	from	%	1263:1263	arg1	treatment					1303:1311	thermal treatment	1295:1311	thermal treatment	1295:1311	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	6	70	from	%	1263:1263	arg1	treatment					1330:1338	combination treatment	1318:1338	combination treatment	1318:1338	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	7	71	theme	flavor	1408:1413	arg1	compounds					1415:1423	the most volatile flavor compounds	1390:1423	the most volatile flavor compounds (38 compounds)	1390:1438	The combination treatment produced the most volatile flavor compounds (38 compounds) at the end of storage (15 days).					
37087226	7	71	theme	flavor	1408:1413	arg1	compounds					1429:1437	38 compounds	1426:1437	38 compounds	1426:1437	The combination treatment produced the most volatile flavor compounds (38 compounds) at the end of storage (15 days).					
37087226	0	72	theme	physicochemical	63:77	arg1	properties					79:88	physicochemical properties	63:88	physicochemical properties	63:88	Effects of chitosan coating combined with thermal treatment on physicochemical properties, bacterial diversity and volatile flavor of braised duck meat during refrigerated storage.					
37087226	7	73	theme	combination	1359:1369	arg1	treatment					1371:1379	The combination treatment	1355:1379	The combination treatment	1355:1379	The combination treatment produced the most volatile flavor compounds (38 compounds) at the end of storage (15 days).					
37087226	8	74	theme	flavor	1551:1556	arg1	1-octen-3-ol					1493:1504	1-octen-3-ol	1493:1504	1-octen-3-ol	1493:1504	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	8	74	theme	flavor	1551:1556	arg1	compounds					1558:1566	the main volatile flavor compounds	1533:1566	the main volatile flavor compounds	1533:1566	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	8	74	theme	flavor	1551:1556	arg1	1-nonanal					1482:1490	1-nonanal	1482:1490	1-nonanal	1482:1490	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	8	74	theme	flavor	1551:1556	arg1	Hexanal					1473:1479	Hexanal	1473:1479	Hexanal	1473:1479	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	8	74	theme	flavor	1551:1556	arg1	2					1511:1511	2	1511:1511	2	1511:1511	Hexanal, 1-nonanal, 1-octen-3-ol, and 2, 3-octanedione were the main volatile flavor compounds, and the average relative peak area was above 80.					
37087226	4	75	theme	%	830:830	arg1	%					836:836	25.8%-78.6%	826:836	25.8%-78.6% (from days 6 to 12)	826:856	Compared with the control, the three preserved treatments reduced TBARS values by 25.8%-78.6% (from days 6 to 12) and total sulfhydryl concentrations by 24.1%-75.7% (from days 3 to 9).					
37087226	6	76	theme	chitosan	1277:1284	arg1	coating					1286:1292	chitosan coating	1277:1292	chitosan coating	1277:1292	The proportions of the four primary spoilage organisms, Brochothrix, Weissella, Acinetobacter, and Pseudomonas, were 74.8%, 76.3%, 70.7%, and 49.7% in control, chitosan coating, thermal treatment, and combination treatment, respectively.					
37087226	1	77	theme	chitosan	229:236	arg1	0.005 g/mL					247:256	0.005 g/mL	247:256	0.005 g/mL	247:256	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	1	77	theme	chitosan	229:236	arg1	coating					238:244	chitosan coating	229:244	chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min)	229:308	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	4	78	theme	sulfhydryl	868:877	arg1	concentrations					879:892	total sulfhydryl concentrations	862:892	total sulfhydryl concentrations	862:892	Compared with the control, the three preserved treatments reduced TBARS values by 25.8%-78.6% (from days 6 to 12) and total sulfhydryl concentrations by 24.1%-75.7% (from days 3 to 9).					
37087226	1	79	dep	treatment	281:289	arg1	85 °C					292:296	85 °C	292:296	85 °C for 30 min	292:307	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
37087226	5	80	theme	synergistic	1097:1107	arg1	effect					1109:1114	a significant synergistic effect	1083:1114	a significant synergistic effect	1083:1114	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	9	81	theme	braised	1722:1728	arg1	meat					1735:1738	braised duck meat	1722:1738	braised duck meat	1722:1738	Therefore, chitosan coating and thermal treatment could be developed as synergistic methods to preserve braised duck meat.					
37087226	5	82	theme	significant	1085:1095	arg1	effect					1109:1114	a significant synergistic effect	1083:1114	a significant synergistic effect	1083:1114	The combination treatment had the lowest values (carbonyl concentration, TVC, and TEC) compared to the chitosan coating and thermal treatment, indicating a significant synergistic effect.					
37087226	1	83	theme	coating	238:244	arg1	impact					219:224	the impact	215:224	the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat	215:428	The current study aimed to assess the impact of chitosan coating (0.005 g/mL) combined with thermal treatment (85 °C for 30 min) on tenderness, lipid and protein oxidation, bacterial diversity, and volatile flavor compounds in braised duck leg meat under vacuum packaging during refrigerated storage (4 °C for 15 days).					
36604095	3	0	theme	excellent	749:757	arg1	compatibility					759:771	excellent compatibility	749:771	excellent compatibility	749:771	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	2	1	from	effect	323:328	arg1	stability					372:380	stability	372:380	stability	372:380	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	2	1	from	effect	323:328	arg1	size					363:366	particle size	354:366	particle size	354:366	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	3	2	theme	WPU	681:683	arg1	stability					668:676	the storage stability	656:676	the storage stability of WPU	656:683	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	3	3	theme	unique	523:528	arg1	structure					530:538	unique structure	523:538	unique structure	523:538	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	4	4	theme	strain	874:879	arg1	sensors					881:887	the flexible strain sensors	861:887	the flexible strain sensors	861:887	By drip-coating silver nanowires (AgNWs) on the surface of the composite films, the flexible strain sensors were fabricated, which showed excellent sensitivity in monitoring human movement.					
36604095	3	5	theme	TCNCs	558:562	arg1	properties					544:553	properties	544:553	properties	544:553	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	3	5	theme	TCNCs	558:562	arg1	structure					530:538	unique structure	523:538	unique structure	523:538	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	1	6	theme	simple	286:291	arg1	solution					293:300	a simple solution	284:300	a simple solution blending method	284:316	In this study, tunicate cellulose nanocrystals (TCNCs) were introduced into castor oil-based waterborne polyurethane (WPU) to prepare bio-based nanocomposites through a simple solution blending method.					
36604095	3	7	theme	high	573:576	arg1	crystallinity					578:590	high crystallinity	573:590	high crystallinity	573:590	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	3	8	theme	reinforcing/toughening	714:735	arg1	effects					737:743	significant reinforcing/toughening effects	702:743	significant reinforcing/toughening effects	702:743	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	4	9	theme	flexible	865:872	arg1	sensors					881:887	the flexible strain sensors	861:887	the flexible strain sensors	861:887	By drip-coating silver nanowires (AgNWs) on the surface of the composite films, the flexible strain sensors were fabricated, which showed excellent sensitivity in monitoring human movement.					
36604095	4	10	theme	drip-coating	784:795	arg1	AgNWs					815:819	AgNWs	815:819	AgNWs	815:819	By drip-coating silver nanowires (AgNWs) on the surface of the composite films, the flexible strain sensors were fabricated, which showed excellent sensitivity in monitoring human movement.					
36604095	4	10	theme	drip-coating	784:795	arg1	nanowires					804:812	drip-coating silver nanowires	784:812	drip-coating silver nanowires (AgNWs) on the surface of the composite films	784:858	By drip-coating silver nanowires (AgNWs) on the surface of the composite films, the flexible strain sensors were fabricated, which showed excellent sensitivity in monitoring human movement.					
36604095	2	11	theme	dispersions	399:409	arg1	stability					372:380	stability	372:380	stability	372:380	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	2	11	theme	dispersions	399:409	arg1	size					363:366	particle size	354:366	particle size	354:366	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	4	12	theme	human	955:959	arg1	movement					961:968	human movement	955:968	human movement	955:968	By drip-coating silver nanowires (AgNWs) on the surface of the composite films, the flexible strain sensors were fabricated, which showed excellent sensitivity in monitoring human movement.					
36604095	2	13	theme	properties	457:466	arg1	stability					372:380	stability	372:380	stability	372:380	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	2	13	theme	properties	457:466	arg1	size					363:366	particle size	354:366	particle size	354:366	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	1	14	theme	castor	193:198	arg1	WPU					235:237	WPU	235:237	WPU	235:237	In this study, tunicate cellulose nanocrystals (TCNCs) were introduced into castor oil-based waterborne polyurethane (WPU) to prepare bio-based nanocomposites through a simple solution blending method.					
36604095	1	14	theme	castor	193:198	arg1	polyurethane					221:232	castor oil-based waterborne polyurethane	193:232	castor oil-based waterborne polyurethane (WPU)	193:238	In this study, tunicate cellulose nanocrystals (TCNCs) were introduced into castor oil-based waterborne polyurethane (WPU) to prepare bio-based nanocomposites through a simple solution blending method.					
36604095	0	15	theme	oil-based	7:15	arg1	nanocrystals					60:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals	0:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals	0:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals nanocomposites for wearable strain sensors.					
36604095	3	16	theme	significant	702:712	arg1	effects					737:743	significant reinforcing/toughening effects	702:743	significant reinforcing/toughening effects	702:743	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	4	17	theme	films	854:858	arg1	surface					829:835	the surface	825:835	the surface of the composite films	825:858	By drip-coating silver nanowires (AgNWs) on the surface of the composite films, the flexible strain sensors were fabricated, which showed excellent sensitivity in monitoring human movement.					
36604095	2	18	theme	content	339:345	arg1	effect					323:328	The effect	319:328	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films	319:489	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	1	19	theme	oil-based	200:208	arg1	WPU					235:237	WPU	235:237	WPU	235:237	In this study, tunicate cellulose nanocrystals (TCNCs) were introduced into castor oil-based waterborne polyurethane (WPU) to prepare bio-based nanocomposites through a simple solution blending method.					
36604095	1	19	theme	oil-based	200:208	arg1	polyurethane					221:232	castor oil-based waterborne polyurethane	193:232	castor oil-based waterborne polyurethane (WPU)	193:238	In this study, tunicate cellulose nanocrystals (TCNCs) were introduced into castor oil-based waterborne polyurethane (WPU) to prepare bio-based nanocomposites through a simple solution blending method.					
36604095	0	20	theme	Castor	0:5	arg1	nanocrystals					60:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals	0:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals	0:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals nanocomposites for wearable strain sensors.					
36604095	4	21	theme	composite	844:852	arg1	films					854:858	the composite films	840:858	the composite films	840:858	By drip-coating silver nanowires (AgNWs) on the surface of the composite films, the flexible strain sensors were fabricated, which showed excellent sensitivity in monitoring human movement.					
36604095	2	22	theme	TCNCs	333:337	arg1	content					339:345	TCNCs content	333:345	TCNCs content	333:345	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	2	23	theme	composite	389:397	arg1	dispersions					399:409	the composite dispersions	385:409	the composite dispersions	385:409	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	0	24	theme	polyurethane/tunicate	28:48	arg1	nanocrystals					60:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals	0:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals	0:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals nanocomposites for wearable strain sensors.					
36604095	3	25	dep	structure	530:538	arg1	only					634:637	only	634:637	only	634:637	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	3	25	dep	structure	530:538	arg1	The					519:521	The	519:521	The	519:521	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	2	26	theme	thermophysical	427:440	arg1	properties					457:466	the thermophysical and mechanical properties	423:466	the thermophysical and mechanical properties of the composite films	423:489	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	0	27	theme	waterborne	17:26	arg1	nanocrystals					60:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals	0:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals	0:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals nanocomposites for wearable strain sensors.					
36604095	2	28	theme	particle	354:361	arg1	size					363:366	particle size	354:366	particle size	354:366	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	3	29	theme	aspect	599:604	arg1	ratio					606:610	large aspect ratio	593:610	large aspect ratio	593:610	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	1	30	theme	waterborne	210:219	arg1	WPU					235:237	WPU	235:237	WPU	235:237	In this study, tunicate cellulose nanocrystals (TCNCs) were introduced into castor oil-based waterborne polyurethane (WPU) to prepare bio-based nanocomposites through a simple solution blending method.					
36604095	1	30	theme	waterborne	210:219	arg1	polyurethane					221:232	castor oil-based waterborne polyurethane	193:232	castor oil-based waterborne polyurethane (WPU)	193:238	In this study, tunicate cellulose nanocrystals (TCNCs) were introduced into castor oil-based waterborne polyurethane (WPU) to prepare bio-based nanocomposites through a simple solution blending method.					
36604095	0	31	theme	cellulose	50:58	arg1	nanocrystals					60:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals	0:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals	0:71	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals nanocomposites for wearable strain sensors.					
36604095	2	32	theme	films	485:489	arg1	properties					457:466	the thermophysical and mechanical properties	423:466	the thermophysical and mechanical properties of the composite films	423:489	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	2	32	theme	films	485:489	arg1	dispersions					399:409	the composite dispersions	385:409	the composite dispersions	385:409	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	2	33	theme	mechanical	446:455	arg1	properties					457:466	the thermophysical and mechanical properties	423:466	the thermophysical and mechanical properties of the composite films	423:489	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	2	34	theme	composite	475:483	arg1	films					485:489	the composite films	471:489	the composite films	471:489	The effect of TCNCs content on the particle size and stability of the composite dispersions, as well as the thermophysical and mechanical properties of the composite films were studied and discussed.					
36604095	1	35	theme	tunicate	132:139	arg1	nanocrystals					151:162	tunicate cellulose nanocrystals	132:162	tunicate cellulose nanocrystals (TCNCs)	132:170	In this study, tunicate cellulose nanocrystals (TCNCs) were introduced into castor oil-based waterborne polyurethane (WPU) to prepare bio-based nanocomposites through a simple solution blending method.					
36604095	1	35	theme	tunicate	132:139	arg1	TCNCs					165:169	TCNCs	165:169	TCNCs	165:169	In this study, tunicate cellulose nanocrystals (TCNCs) were introduced into castor oil-based waterborne polyurethane (WPU) to prepare bio-based nanocomposites through a simple solution blending method.					
36604095	4	36	theme	excellent	919:927	arg1	sensitivity					929:939	excellent sensitivity	919:939	excellent sensitivity in monitoring human movement	919:968	By drip-coating silver nanowires (AgNWs) on the surface of the composite films, the flexible strain sensors were fabricated, which showed excellent sensitivity in monitoring human movement.					
36604095	3	37	theme	large	593:597	arg1	aspect					599:604	large aspect	593:604	large aspect ratio	593:610	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	1	38	theme	cellulose	141:149	arg1	nanocrystals					151:162	tunicate cellulose nanocrystals	132:162	tunicate cellulose nanocrystals (TCNCs)	132:170	In this study, tunicate cellulose nanocrystals (TCNCs) were introduced into castor oil-based waterborne polyurethane (WPU) to prepare bio-based nanocomposites through a simple solution blending method.					
36604095	1	38	theme	cellulose	141:149	arg1	TCNCs					165:169	TCNCs	165:169	TCNCs	165:169	In this study, tunicate cellulose nanocrystals (TCNCs) were introduced into castor oil-based waterborne polyurethane (WPU) to prepare bio-based nanocomposites through a simple solution blending method.					
36604095	4	39	theme	silver	797:802	arg1	AgNWs					815:819	AgNWs	815:819	AgNWs	815:819	By drip-coating silver nanowires (AgNWs) on the surface of the composite films, the flexible strain sensors were fabricated, which showed excellent sensitivity in monitoring human movement.					
36604095	4	39	theme	silver	797:802	arg1	nanowires					804:812	drip-coating silver nanowires	784:812	drip-coating silver nanowires (AgNWs) on the surface of the composite films	784:858	By drip-coating silver nanowires (AgNWs) on the surface of the composite films, the flexible strain sensors were fabricated, which showed excellent sensitivity in monitoring human movement.					
36604095	0	40	theme	strain	101:106	arg1	sensors					108:114	wearable strain sensors	92:114	wearable strain sensors	92:114	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals nanocomposites for wearable strain sensors.					
36604095	3	41	theme	storage	660:666	arg1	stability					668:676	the storage stability	656:676	the storage stability of WPU	656:683	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	1	42	theme	bio-based	251:259	arg1	nanocomposites					261:274	bio-based nanocomposites	251:274	bio-based nanocomposites	251:274	In this study, tunicate cellulose nanocrystals (TCNCs) were introduced into castor oil-based waterborne polyurethane (WPU) to prepare bio-based nanocomposites through a simple solution blending method.					
36604095	0	43	theme	wearable	92:99	arg1	sensors					108:114	wearable strain sensors	92:114	wearable strain sensors	92:114	Castor oil-based waterborne polyurethane/tunicate cellulose nanocrystals nanocomposites for wearable strain sensors.					
36604095	3	44	theme	high	616:619	arg1	modulus					621:627	high modulus	616:627	high modulus	616:627	The unique structure and properties of TCNCs, such as high crystallinity, large aspect ratio and high modulus, not only greatly improved the storage stability of WPU, but also showed significant reinforcing/toughening effects and excellent compatibility to WPU.					
36604095	4	45	from	nanowires	804:812	arg1	surface					829:835	the surface	825:835	the surface of the composite films	825:858	By drip-coating silver nanowires (AgNWs) on the surface of the composite films, the flexible strain sensors were fabricated, which showed excellent sensitivity in monitoring human movement.					
35760001	4	0	theme	daily	812:816	arg1	gain					825:828	daily weight gain	812:828	daily weight gain (DWG)	812:834	BW, daily feed intake (DFI), daily weight gain (DWG), feed conversion rate (FCR), and mortality were measured.					
35760001	4	0	theme	daily	812:816	arg1	DWG					831:833	DWG	831:833	DWG	831:833	BW, daily feed intake (DFI), daily weight gain (DWG), feed conversion rate (FCR), and mortality were measured.					
35760001	2	1	theme	egg	327:329	arg1	incubation					331:340	egg incubation	327:340	egg incubation	327:340	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	11	2	theme	Proximate	1520:1528	arg1	composition					1530:1540	C. Proximate composition	1517:1540	C. Proximate composition	1517:1540	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	5	3	theme	selected	922:929	arg1	conditions					961:970	15 randomly selected birds/treatment/environmental conditions	910:970	15 randomly selected birds/treatment/environmental conditions	910:970	At 50 d of age, 15 randomly selected birds/treatment/environmental conditions were slaughtered and the pectoral muscle (PM) was collected for analyses.					
35760001	7	4	theme	C.	1156:1157	arg1	BW					1165:1166	C. Final BW	1156:1166	C. Final BW	1156:1166	BW of the newly hatched chicks was lower (P < 0.01) in GOS compared to C. Final BW, DWG, DFI, and FCR were not affected (P > 0.05) by GOS.					
35760001	11	5	theme	fatty	1564:1568	arg1	profile					1575:1581	fatty acid profile	1564:1581	fatty acid profile	1564:1581	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	9	6	theme	finisher	1273:1280	arg1	phase					1282:1286	finisher phase	1273:1286	finisher phase	1273:1286	During finisher phase, DFI and DWG were lower (P < 0.001) and FCR was higher (P < 0.01) in HS compared to TN.					
35760001	11	7	theme	cholesterol	1543:1553	arg1	content					1555:1561	cholesterol content	1543:1561	cholesterol content	1543:1561	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	2	8	theme	physiological	481:493	arg1	saline					495:500	physiological saline	481:500	physiological saline (only to assess the hatchability rate)	481:539	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	4	9	theme	conversion	842:851	arg1	FCR					859:861	FCR	859:861	FCR	859:861	BW, daily feed intake (DFI), daily weight gain (DWG), feed conversion rate (FCR), and mortality were measured.					
35760001	4	9	theme	conversion	842:851	arg1	rate					853:856	feed conversion rate	837:856	feed conversion rate (FCR)	837:862	BW, daily feed intake (DFI), daily weight gain (DWG), feed conversion rate (FCR), and mortality were measured.					
35760001	0	10	theme	heat	123:126	arg1	stress					128:133	heat stress	123:133	heat stress	123:133	Performance and meat quality traits of slow-growing chickens stimulated in ovo with galactooligosaccharides and exposed to heat stress.					
35760001	1	11	theme	slow-growing	192:203	arg1	chickens					205:212	slow-growing chickens	192:212	slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress	192:298	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	12	12	theme	collagen	1709:1716	arg1	content					1693:1699	a lower (P < 0.05) content	1674:1699	a lower (P < 0.05) content of both collagen and monounsaturated fatty acids	1674:1748	The HS group showed a lower (P < 0.05) content of both collagen and monounsaturated fatty acids than TN group.					
35760001	7	13	theme	hatched	1101:1107	arg1	chicks					1109:1114	the newly hatched chicks	1091:1114	the newly hatched chicks	1091:1114	BW of the newly hatched chicks was lower (P < 0.01) in GOS compared to C. Final BW, DWG, DFI, and FCR were not affected (P > 0.05) by GOS.					
35760001	11	14	from	chickens	1443:1450	arg1	Meat					1429:1432	Meat	1429:1432	Meat from GOS chickens	1429:1450	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	12	15	theme	monounsaturated	1722:1736	arg1	acids					1744:1748	monounsaturated fatty acids	1722:1748	monounsaturated fatty acids	1722:1748	The HS group showed a lower (P < 0.05) content of both collagen and monounsaturated fatty acids than TN group.					
35760001	14	16	theme	mortality	1969:1977	arg1	rate					1979:1982	mortality rate	1969:1982	mortality rate	1969:1982	In conclusion, the differences in performance have had an impact on the responses to HS in Hubbard chickens, but not on mortality rate.					
35760001	1	17	theme	In	136:137	arg1	performance					144:154	In vivo performance	136:154	In vivo performance	136:154	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	9	18	theme	<	1315:1315	arg1	P					1313:1313	P < 0.001	1313:1321	P < 0.001	1313:1321	During finisher phase, DFI and DWG were lower (P < 0.001) and FCR was higher (P < 0.01) in HS compared to TN.					
35760001	3	19	theme	floor	655:659	arg1	pens					661:664	floor pens	655:664	floor pens (6 pens/treatment, 25 birds/pen)	655:697	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	7	20	dep	lower	1120:1124	arg1	<					1129:1129	P < 0.01	1127:1134	P < 0.01	1127:1134	BW of the newly hatched chicks was lower (P < 0.01) in GOS compared to C. Final BW, DWG, DFI, and FCR were not affected (P > 0.05) by GOS.					
35760001	2	21	theme	mg	438:439	arg1	GOS/egg					441:447	3.5 mg GOS/egg	434:447	3.5 mg GOS/egg	434:447	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	14	22	from	differences	1868:1878	arg1	performance					1883:1893	performance	1883:1893	performance	1883:1893	In conclusion, the differences in performance have had an impact on the responses to HS in Hubbard chickens, but not on mortality rate.					
35760001	11	23	dep	darker	1487:1492	arg1	<					1497:1497	P < 0.05	1495:1502	P < 0.05	1495:1502	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	3	24	theme	neutral	716:722	arg1	conditions					744:753	neutral (TN) or heat stress conditions	716:753	neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d)	716:780	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	14	25	theme	Hubbard	1940:1946	arg1	chickens					1948:1955	Hubbard chickens	1940:1955	Hubbard chickens	1940:1955	In conclusion, the differences in performance have had an impact on the responses to HS in Hubbard chickens, but not on mortality rate.					
35760001	15	26	theme	HS	2028:2029	arg1	effect					2018:2023	the negative effect	2005:2023	the negative effect of HS on chickens' performance	2005:2054	GOS did not relieve the negative effect of HS on chickens' performance.					
35760001	8	27	dep	BW	1241:1242	arg1	P					1254:1254	P	1254:1254	P	1254:1254	HS reduced final BW (-12.93%, P < 0.001).					
35760001	8	27	dep	BW	1241:1242	arg1	%					1251:1251	-12.93%	1245:1251	-12.93%	1245:1251	HS reduced final BW (-12.93%, P < 0.001).					
35760001	3	28	theme	stress	737:742	arg1	conditions					744:753	neutral (TN) or heat stress conditions	716:753	neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d)	716:780	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	7	29	theme	P	1206:1206	arg1	>					1208:1208	P > 0.05	1206:1213	P > 0.05	1206:1213	BW of the newly hatched chicks was lower (P < 0.01) in GOS compared to C. Final BW, DWG, DFI, and FCR were not affected (P > 0.05) by GOS.					
35760001	2	30	theme	control	559:565	arg1	C					574:574	C	574:574	C	574:574	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	2	30	theme	control	559:565	arg1	group					567:571	an uninjected control group	545:571	an uninjected control group (C)	545:575	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	12	31	theme	HS	1658:1659	arg1	group					1661:1665	The HS group	1654:1665	The HS group	1654:1665	The HS group showed a lower (P < 0.05) content of both collagen and monounsaturated fatty acids than TN group.					
35760001	9	32	dep	lower	1306:1310	arg1	P					1313:1313	P < 0.001	1313:1321	P < 0.001	1313:1321	During finisher phase, DFI and DWG were lower (P < 0.001) and FCR was higher (P < 0.01) in HS compared to TN.					
35760001	3	33	dep	neutral	716:722	arg1	TN					725:726	TN	725:726	TN	725:726	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	9	34	theme	P	1344:1344	arg1	<					1346:1346	P < 0.01	1344:1351	P < 0.01	1344:1351	During finisher phase, DFI and DWG were lower (P < 0.001) and FCR was higher (P < 0.01) in HS compared to TN.					
35760001	1	35	theme	heat	288:291	arg1	stress					293:298	heat stress	288:298	heat stress	288:298	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	12	36	dep	lower	1676:1680	arg1	<					1685:1685	P < 0.05	1683:1690	P < 0.05	1683:1690	The HS group showed a lower (P < 0.05) content of both collagen and monounsaturated fatty acids than TN group.					
35760001	2	37	theme	uninjected	548:557	arg1	C					574:574	C	574:574	C	574:574	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	2	37	theme	uninjected	548:557	arg1	group					567:571	an uninjected control group	545:571	an uninjected control group (C)	545:575	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	2	38	theme	prebiotic	398:406	arg1	group					408:412	prebiotic group	398:412	prebiotic group (GOS) injected with 3.5 mg GOS/egg	398:447	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	2	38	theme	prebiotic	398:406	arg1	GOS					415:417	GOS	415:417	GOS	415:417	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	0	39	theme	meat	16:19	arg1	traits					29:34	meat quality traits	16:34	meat quality traits	16:34	Performance and meat quality traits of slow-growing chickens stimulated in ovo with galactooligosaccharides and exposed to heat stress.					
35760001	4	40	theme	daily	787:791	arg1	DFI					806:808	DFI	806:808	DFI	806:808	BW, daily feed intake (DFI), daily weight gain (DWG), feed conversion rate (FCR), and mortality were measured.					
35760001	4	40	theme	daily	787:791	arg1	intake					798:803	daily feed intake	787:803	daily feed intake (DFI)	787:809	BW, daily feed intake (DFI), daily weight gain (DWG), feed conversion rate (FCR), and mortality were measured.					
35760001	13	41	theme	Significant	1765:1775	arg1	interactions					1777:1788	Significant interactions	1765:1788	Significant interactions between GOS and temperature	1765:1816	Significant interactions between GOS and temperature were found for FA composition.					
35760001	11	42	theme	collagen	1602:1609	arg1	properties					1611:1620	intramuscular collagen properties	1588:1620	intramuscular collagen properties	1588:1620	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	0	43	theme	slow-growing	39:50	arg1	chickens					52:59	slow-growing chickens	39:59	slow-growing chickens	39:59	Performance and meat quality traits of slow-growing chickens stimulated in ovo with galactooligosaccharides and exposed to heat stress.					
35760001	14	44	from	impact	1907:1912	arg1	responses					1921:1929	the responses	1917:1929	the responses to HS in Hubbard chickens	1917:1955	In conclusion, the differences in performance have had an impact on the responses to HS in Hubbard chickens, but not on mortality rate.					
35760001	14	44	from	impact	1907:1912	arg1	rate					1979:1982	mortality rate	1969:1982	mortality rate	1969:1982	In conclusion, the differences in performance have had an impact on the responses to HS in Hubbard chickens, but not on mortality rate.					
35760001	1	45	dep	chickens	205:212	arg1	exposed					277:283	exposed	277:283	exposed to heat stress	277:298	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	1	45	dep	chickens	205:212	arg1	stimulated					214:223	stimulated	214:223	stimulated in ovo with trans galactooligosaccharides (GOS)	214:271	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	5	46	theme	birds/treatment/environmental	931:959	arg1	conditions					961:970	15 randomly selected birds/treatment/environmental conditions	910:970	15 randomly selected birds/treatment/environmental conditions	910:970	At 50 d of age, 15 randomly selected birds/treatment/environmental conditions were slaughtered and the pectoral muscle (PM) was collected for analyses.					
35760001	4	47	theme	feed	837:840	arg1	FCR					859:861	FCR	859:861	FCR	859:861	BW, daily feed intake (DFI), daily weight gain (DWG), feed conversion rate (FCR), and mortality were measured.					
35760001	4	47	theme	feed	837:840	arg1	rate					853:856	feed conversion rate	837:856	feed conversion rate (FCR)	837:862	BW, daily feed intake (DFI), daily weight gain (DWG), feed conversion rate (FCR), and mortality were measured.					
35760001	2	48	theme	3,000	343:347	arg1	Hubbard					366:372	Hubbard	366:372	Hubbard	366:372	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	2	48	theme	3,000	343:347	arg1	eggs					360:363	3,000 fertilized eggs	343:363	3,000 fertilized eggs (Hubbard JA57)	343:378	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	2	49	dep	saline	495:500	arg1	assess					511:516	assess	511:516	to assess the hatchability rate	508:538	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	4	50	theme	weight	818:823	arg1	gain					825:828	daily weight gain	812:828	daily weight gain (DWG)	812:834	BW, daily feed intake (DFI), daily weight gain (DWG), feed conversion rate (FCR), and mortality were measured.					
35760001	4	50	theme	weight	818:823	arg1	DWG					831:833	DWG	831:833	DWG	831:833	BW, daily feed intake (DFI), daily weight gain (DWG), feed conversion rate (FCR), and mortality were measured.					
35760001	8	51	theme	final	1235:1239	arg1	BW					1241:1242	final BW	1235:1242	final BW (-12.93%, P < 0.001)	1235:1263	HS reduced final BW (-12.93%, P < 0.001).					
35760001	2	52	theme	incubation	331:340	arg1	d					319:319	d 12	319:322	d 12 of egg incubation	319:340	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	7	53	theme	Final	1159:1163	arg1	BW					1165:1166	C. Final BW	1156:1166	C. Final BW	1156:1166	BW of the newly hatched chicks was lower (P < 0.01) in GOS compared to C. Final BW, DWG, DFI, and FCR were not affected (P > 0.05) by GOS.					
35760001	2	54	theme	hatchability	522:533	arg1	rate					535:538	the hatchability rate	518:538	the hatchability rate	518:538	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	12	55	theme	acids	1744:1748	arg1	content					1693:1699	a lower (P < 0.05) content	1674:1699	a lower (P < 0.05) content of both collagen and monounsaturated fatty acids	1674:1748	The HS group showed a lower (P < 0.05) content of both collagen and monounsaturated fatty acids than TN group.					
35760001	0	56	with	ovo	75:77	arg1	galactooligosaccharides					84:106	galactooligosaccharides	84:106	galactooligosaccharides	84:106	Performance and meat quality traits of slow-growing chickens stimulated in ovo with galactooligosaccharides and exposed to heat stress.					
35760001	12	57	theme	TN	1755:1756	arg1	group					1758:1762	TN group	1755:1762	TN group	1755:1762	The HS group showed a lower (P < 0.05) content of both collagen and monounsaturated fatty acids than TN group.					
35760001	11	58	theme	P	1466:1466	arg1	<					1468:1468	P < 0.01	1466:1473	P < 0.01	1466:1473	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	11	59	theme	acid	1570:1573	arg1	profile					1575:1581	fatty acid profile	1564:1581	fatty acid profile	1564:1581	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	3	60	dep	50	776:777	arg1	to					773:774	to	773:774	to	773:774	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	1	61	theme	quality	174:180	arg1	traits					182:187	quality traits	174:187	quality traits	174:187	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	11	62	contain	had	1452:1454	arg1	Meat					1429:1432	Meat	1429:1432	Meat from GOS chickens	1429:1450	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	11	62	contain	had	1452:1454	arg2	pH					1476:1477	a higher (P < 0.01) pH	1456:1477	a higher (P < 0.01) pH	1456:1477	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	7	63	theme	P	1127:1127	arg1	<					1129:1129	P < 0.01	1127:1134	P < 0.01	1127:1134	BW of the newly hatched chicks was lower (P < 0.01) in GOS compared to C. Final BW, DWG, DFI, and FCR were not affected (P > 0.05) by GOS.					
35760001	3	64	dep	pens	661:664	arg1	pens/treatment					669:682	6 pens/treatment	667:682	6 pens/treatment	667:682	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	3	64	dep	pens	661:664	arg1	birds/pen					688:696	25 birds/pen	685:696	25 birds/pen	685:696	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	11	65	theme	GOS	1439:1441	arg1	chickens					1443:1450	GOS chickens	1439:1450	GOS chickens	1439:1450	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	1	66	theme	chickens	205:212	arg1	performance					144:154	In vivo performance	136:154	In vivo performance	136:154	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	1	66	theme	chickens	205:212	arg1	meat					169:172	meat	169:172	meat	169:172	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	1	66	theme	chickens	205:212	arg1	carcass					157:163	carcass	157:163	carcass	157:163	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	2	67	dep	assess	511:516	arg1	only					503:506	only	503:506	only	503:506	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	11	68	theme	P	1495:1495	arg1	<					1497:1497	P < 0.05	1495:1502	P < 0.05	1495:1502	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	12	69	theme	fatty	1738:1742	arg1	acids					1744:1748	monounsaturated fatty acids	1722:1748	monounsaturated fatty acids	1722:1748	The HS group showed a lower (P < 0.05) content of both collagen and monounsaturated fatty acids than TN group.					
35760001	7	70	theme	chicks	1109:1114	arg1	BW					1085:1086	BW	1085:1086	BW of the newly hatched chicks	1085:1114	BW of the newly hatched chicks was lower (P < 0.01) in GOS compared to C. Final BW, DWG, DFI, and FCR were not affected (P > 0.05) by GOS.					
35760001	9	71	dep	higher	1336:1341	arg1	<					1346:1346	P < 0.01	1344:1351	P < 0.01	1344:1351	During finisher phase, DFI and DWG were lower (P < 0.001) and FCR was higher (P < 0.01) in HS compared to TN.					
35760001	11	72	theme	C.	1517:1518	arg1	composition					1530:1540	C. Proximate composition	1517:1540	C. Proximate composition	1517:1540	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	11	73	theme	higher	1458:1463	arg1	pH					1476:1477	a higher (P < 0.01) pH	1456:1477	a higher (P < 0.01) pH	1456:1477	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	3	74	dep	conditions	744:753	arg1	HS					756:757	HS	756:757	HS	756:757	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	3	74	dep	conditions	744:753	arg1	30°C					760:763	30°C	760:763	30°C from 36 to 50 d	760:779	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	12	75	theme	P	1683:1683	arg1	<					1685:1685	P < 0.05	1683:1690	P < 0.05	1683:1690	The HS group showed a lower (P < 0.05) content of both collagen and monounsaturated fatty acids than TN group.					
35760001	8	76	dep	%	1251:1251	arg1	0.001					1258:1262	0.001	1258:1262	0.001	1258:1262	HS reduced final BW (-12.93%, P < 0.001).					
35760001	2	77	theme	3.5	434:436	arg1	mg					438:439	mg	438:439	mg	438:439	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	5	78	theme	pectoral	997:1004	arg1	PM					1014:1015	PM	1014:1015	PM	1014:1015	At 50 d of age, 15 randomly selected birds/treatment/environmental conditions were slaughtered and the pectoral muscle (PM) was collected for analyses.					
35760001	5	78	theme	pectoral	997:1004	arg1	muscle					1006:1011	the pectoral muscle	993:1011	the pectoral muscle (PM)	993:1016	At 50 d of age, 15 randomly selected birds/treatment/environmental conditions were slaughtered and the pectoral muscle (PM) was collected for analyses.					
35760001	1	79	dep	In	136:137	arg1	vivo					139:142	vivo	139:142	vivo	139:142	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	15	80	theme	negative	2009:2016	arg1	effect					2018:2023	the negative effect	2005:2023	the negative effect of HS on chickens' performance	2005:2054	GOS did not relieve the negative effect of HS on chickens' performance.					
35760001	13	81	theme	FA	1833:1834	arg1	composition					1836:1846	FA composition	1833:1846	FA composition	1833:1846	Significant interactions between GOS and temperature were found for FA composition.					
35760001	3	82	from	d	779:779	arg1	HS					756:757	HS	756:757	HS	756:757	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	3	82	from	d	779:779	arg1	30°C					760:763	30°C	760:763	30°C from 36 to 50 d	760:779	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	2	83	theme	saline	450:455	arg1	group					457:461	saline group	450:461	saline group (S) injected with physiological saline (only to assess the hatchability rate)	450:539	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	2	83	theme	saline	450:455	arg1	S					464:464	S	464:464	S	464:464	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	12	84	theme	lower	1676:1680	arg1	content					1693:1699	a lower (P < 0.05) content	1674:1699	a lower (P < 0.05) content of both collagen and monounsaturated fatty acids	1674:1748	The HS group showed a lower (P < 0.05) content of both collagen and monounsaturated fatty acids than TN group.					
35760001	3	85	theme	heat	732:735	arg1	conditions					744:753	neutral (TN) or heat stress conditions	716:753	neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d)	716:780	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	11	86	theme	intramuscular	1588:1600	arg1	properties					1611:1620	intramuscular collagen properties	1588:1620	intramuscular collagen properties	1588:1620	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	2	87	theme	fertilized	349:358	arg1	Hubbard					366:372	Hubbard	366:372	Hubbard	366:372	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	2	87	theme	fertilized	349:358	arg1	eggs					360:363	3,000 fertilized eggs	343:363	3,000 fertilized eggs (Hubbard JA57)	343:378	On d 12 of egg incubation, 3,000 fertilized eggs (Hubbard JA57) were divided into prebiotic group (GOS) injected with 3.5 mg GOS/egg, saline group (S) injected with physiological saline (only to assess the hatchability rate) and an uninjected control group (C).					
35760001	0	88	theme	quality	21:27	arg1	traits					29:34	meat quality traits	16:34	meat quality traits	16:34	Performance and meat quality traits of slow-growing chickens stimulated in ovo with galactooligosaccharides and exposed to heat stress.					
35760001	1	89	dep	performance	144:154	arg1	traits					182:187	quality traits	174:187	quality traits	174:187	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	5	90	theme	age	905:907	arg1	d					900:900	50 d	897:900	50 d of age	897:907	At 50 d of age, 15 randomly selected birds/treatment/environmental conditions were slaughtered and the pectoral muscle (PM) was collected for analyses.					
35760001	3	91	dep	chicks	603:608	arg1	300					611:613	300	611:613	300	611:613	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	3	91	dep	chicks	603:608	arg1	300					628:630	300	628:630	300	628:630	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	11	92	dep	higher	1458:1463	arg1	<					1468:1468	P < 0.01	1466:1473	P < 0.01	1466:1473	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	11	93	theme	PM	1625:1626	arg1	properties					1611:1620	intramuscular collagen properties	1588:1620	intramuscular collagen properties	1588:1620	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	11	93	theme	PM	1625:1626	arg1	profile					1575:1581	fatty acid profile	1564:1581	fatty acid profile	1564:1581	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	11	93	theme	PM	1625:1626	arg1	composition					1530:1540	C. Proximate composition	1517:1540	C. Proximate composition	1517:1540	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	11	93	theme	PM	1625:1626	arg1	content					1555:1561	cholesterol content	1543:1561	cholesterol content	1543:1561	Meat from GOS chickens had a higher (P < 0.01) pH and was darker (P < 0.05) compared to C. Proximate composition, cholesterol content, fatty acid profile, and intramuscular collagen properties of PM were not affected by GOS.					
35760001	14	94	from	responses	1921:1929	arg1	chickens					1948:1955	Hubbard chickens	1940:1955	Hubbard chickens	1940:1955	In conclusion, the differences in performance have had an impact on the responses to HS in Hubbard chickens, but not on mortality rate.					
35760001	0	95	theme	chickens	52:59	arg1	Performance					0:10	Performance	0:10	Performance	0:10	Performance and meat quality traits of slow-growing chickens stimulated in ovo with galactooligosaccharides and exposed to heat stress.					
35760001	0	95	theme	chickens	52:59	arg1	traits					29:34	meat quality traits	16:34	meat quality traits	16:34	Performance and meat quality traits of slow-growing chickens stimulated in ovo with galactooligosaccharides and exposed to heat stress.					
35760001	4	96	theme	feed	793:796	arg1	DFI					806:808	DFI	806:808	DFI	806:808	BW, daily feed intake (DFI), daily weight gain (DWG), feed conversion rate (FCR), and mortality were measured.					
35760001	4	96	theme	feed	793:796	arg1	intake					798:803	daily feed intake	787:803	daily feed intake (DFI)	787:809	BW, daily feed intake (DFI), daily weight gain (DWG), feed conversion rate (FCR), and mortality were measured.					
35760001	10	97	theme	P	1404:1404	arg1	>					1406:1406	P > 0.05	1404:1411	P > 0.05	1404:1411	Mortality was not affected (P > 0.05) by GOS and HS.					
35760001	10	98	dep	affected	1394:1401	arg1	>					1406:1406	P > 0.05	1404:1411	P > 0.05	1404:1411	Mortality was not affected (P > 0.05) by GOS and HS.					
35760001	3	99	theme	male	598:601	arg1	chicks					603:608	600 male chicks	594:608	600 male chicks (300 from GOS and 300 from C)	594:638	After hatching, 600 male chicks (300 from GOS and 300 from C) were housed on floor pens (6 pens/treatment, 25 birds/pen) and reared under neutral (TN) or heat stress conditions (HS, 30°C from 36 to 50 d).					
35760001	1	100	theme	trans	237:241	arg1	GOS					268:270	GOS	268:270	GOS	268:270	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	1	100	theme	trans	237:241	arg1	galactooligosaccharides					243:265	trans galactooligosaccharides	237:265	trans galactooligosaccharides (GOS)	237:271	In vivo performance, carcass and meat quality traits of slow-growing chickens stimulated in ovo with trans galactooligosaccharides (GOS) and exposed to heat stress were evaluated.					
35760001	7	101	dep	affected	1196:1203	arg1	>					1208:1208	P > 0.05	1206:1213	P > 0.05	1206:1213	BW of the newly hatched chicks was lower (P < 0.01) in GOS compared to C. Final BW, DWG, DFI, and FCR were not affected (P > 0.05) by GOS.					
36972825	0	0	theme	metal	116:120	arg1	capture					127:133	capture	127:133	capture	127:133	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	0	0	theme	metal	116:120	arg1	ions					122:125	enhanced heavy metal ions capture and immobilization	101:152	enhanced heavy metal ions capture and immobilization	101:152	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	0	0	theme	metal	116:120	arg1	immobilization					139:152	immobilization	139:152	immobilization	139:152	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	2	1	theme	layered-net	474:484	arg1	structure					486:494	a layered-net structure	472:494	a layered-net structure	472:494	A variety of characterizations show that cellulose nanofiber (CNF) formed a layered-net structure.					
36972825	4	2	from	dots	571:574	arg1	size					601:604	size	601:604	size	601:604	Carbon dots (CDs) around 10-20 nm in size were produced from CNF and distributed along CNF.					
36972825	1	3	theme	heavy	185:189	arg1	HMIs					203:206	HMIs	203:206	HMIs	203:206	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	1	3	theme	heavy	185:189	arg1	ions					197:200	heavy metal ions	185:200	heavy metal ions (HMIs)	185:207	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	11	4	theme	contamination	1281:1293	arg1	risk					1263:1266	the risk	1259:1266	the risk of secondary contamination	1259:1293	It achieves the facile separation and recovery of HMIs from the sewage, and more importantly, eliminates the risk of secondary contamination.					
36972825	1	5	theme	high-efficient-sewage	257:277	arg1	agent					289:293	a high-efficient-sewage treatment agent	255:293	a high-efficient-sewage treatment agent	255:293	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	1	5	theme	high-efficient-sewage	257:277	arg1	OH					331:332	carbon dots/cellulose nanofiber/Mg(OH)2	296:334	carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg)	296:341	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	0	6	theme	heavy	110:114	arg1	capture					127:133	capture	127:133	capture	127:133	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	0	6	theme	heavy	110:114	arg1	ions					122:125	enhanced heavy metal ions capture and immobilization	101:152	enhanced heavy metal ions capture and immobilization	101:152	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	0	6	theme	heavy	110:114	arg1	immobilization					139:152	immobilization	139:152	immobilization	139:152	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	8	7	theme	water	943:947	arg1	qualification					913:925	the qualification	909:925	the qualification of the drinking water	909:947	Notably, the qualification of the drinking water can be satisfied while applying CCMg to handle Cu2+ wastewater.					
36972825	1	8	theme	metal	191:195	arg1	HMIs					203:206	HMIs	203:206	HMIs	203:206	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	1	8	theme	metal	191:195	arg1	ions					197:200	heavy metal ions	185:200	heavy metal ions (HMIs)	185:207	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	1	9	theme	treatment	279:287	arg1	agent					289:293	a high-efficient-sewage treatment agent	255:293	a high-efficient-sewage treatment agent	255:293	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	1	9	theme	treatment	279:287	arg1	OH					331:332	carbon dots/cellulose nanofiber/Mg(OH)2	296:334	carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg)	296:341	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	5	10	theme	structural	674:683	arg1	feature					685:691	The extraordinary structural feature	656:691	The extraordinary structural feature	656:691	The extraordinary structural feature endows CCMg with high removal performance towards HMIs.					
36972825	7	11	contain	bears	849:853	arg1	composite					839:847	The composite	835:847	The composite	835:847	The composite bears excellent durability in treating wastewater.					
36972825	7	11	contain	bears	849:853	arg2	durability					865:874	excellent durability	855:874	excellent durability	855:874	The composite bears excellent durability in treating wastewater.					
36972825	8	12	theme	drinking	934:941	arg1	water					943:947	the drinking water	930:947	the drinking water	930:947	Notably, the qualification of the drinking water can be satisfied while applying CCMg to handle Cu2+ wastewater.					
36972825	3	13	attach	attached	547:554	arg3	CNF					559:561	CNF	559:561	CNF	559:561	Hexagonal Mg(OH)2 flakes of about 100 nm has been attached on CNF.					
36972825	3	13	attach	attached	547:554	arg2	flakes					515:520	Hexagonal Mg(OH)2 flakes	497:520	Hexagonal Mg(OH)2 flakes of about 100 nm	497:536	Hexagonal Mg(OH)2 flakes of about 100 nm has been attached on CNF.					
36972825	8	14	theme	Cu2+	996:999	arg1	wastewater					1001:1010	Cu2+ wastewater	996:1010	Cu2+ wastewater	996:1010	Notably, the qualification of the drinking water can be satisfied while applying CCMg to handle Cu2+ wastewater.					
36972825	3	15	theme	Hexagonal	497:505	arg1	Mg					507:508	Hexagonal Mg	497:508	Hexagonal Mg(OH)2 flakes of about 100 nm	497:536	Hexagonal Mg(OH)2 flakes of about 100 nm has been attached on CNF.					
36972825	3	15	theme	Hexagonal	497:505	arg1	OH					510:511	OH	510:511	OH	510:511	Hexagonal Mg(OH)2 flakes of about 100 nm has been attached on CNF.					
36972825	9	16	theme	removal	1030:1036	arg1	process					1038:1044	removal process	1030:1044	removal process	1030:1044	The mechanism of removal process has been proposed.					
36972825	6	17	theme	up-taken	753:760	arg1	capacities					762:771	The up-taken capacities	749:771	The up-taken capacities	749:771	The up-taken capacities reach 992.8 and 667.3 mg g-1 for Cd2+ and Cu2+, respectively.					
36972825	9	18	theme	process	1038:1044	arg1	mechanism					1017:1025	The mechanism	1013:1025	The mechanism of removal process	1013:1044	The mechanism of removal process has been proposed.					
36972825	1	19	theme	ions	197:200	arg1	problem					174:180	pollution problem	164:180	pollution problem of heavy metal ions (HMIs)	164:207	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	10	20	theme	confinement	1134:1144	arg1	effect					1146:1151	the space confinement effect	1124:1151	the space confinement effect	1124:1151	Practically, Cd2+/Cu2+ ions were immobilized by CNF due to the space confinement effect.					
36972825	0	21	dep	ions	122:125	arg1	capture					127:133	capture	127:133	capture	127:133	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	0	21	dep	ions	122:125	arg1	ions					122:125	enhanced heavy metal ions capture and immobilization	101:152	enhanced heavy metal ions capture and immobilization	101:152	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	0	21	dep	ions	122:125	arg1	immobilization					139:152	immobilization	139:152	immobilization	139:152	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	11	22	theme	secondary	1271:1279	arg1	contamination					1281:1293	secondary contamination	1271:1293	secondary contamination	1271:1293	It achieves the facile separation and recovery of HMIs from the sewage, and more importantly, eliminates the risk of secondary contamination.					
36972825	0	23	theme	Confinement	0:10	arg1	effect					12:17	Confinement effect	0:17	Confinement effect of network-structured carbon	0:46	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	1	24	theme	carbon	296:301	arg1	CCMg					337:340	CCMg	337:340	CCMg	337:340	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	1	24	theme	carbon	296:301	arg1	agent					289:293	a high-efficient-sewage treatment agent	255:293	a high-efficient-sewage treatment agent	255:293	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	1	24	theme	carbon	296:301	arg1	OH					331:332	carbon dots/cellulose nanofiber/Mg(OH)2	296:334	carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg)	296:341	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	0	25	theme	network-structured	22:39	arg1	carbon					41:46	network-structured carbon	22:46	network-structured carbon	22:46	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	1	26	theme	dots/cellulose	303:316	arg1	CCMg					337:340	CCMg	337:340	CCMg	337:340	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	1	26	theme	dots/cellulose	303:316	arg1	agent					289:293	a high-efficient-sewage treatment agent	255:293	a high-efficient-sewage treatment agent	255:293	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	1	26	theme	dots/cellulose	303:316	arg1	OH					331:332	carbon dots/cellulose nanofiber/Mg(OH)2	296:334	carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg)	296:341	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	5	27	theme	removal	715:721	arg1	performance					723:733	high removal performance	710:733	high removal performance towards HMIs	710:746	The extraordinary structural feature endows CCMg with high removal performance towards HMIs.					
36972825	2	28	theme	characterizations	411:427	arg1	variety					400:406	A variety	398:406	A variety of characterizations	398:427	A variety of characterizations show that cellulose nanofiber (CNF) formed a layered-net structure.					
36972825	2	28	theme	characterizations	411:427	arg1	characterizations					411:427	characterizations	411:427	characterizations	411:427	A variety of characterizations show that cellulose nanofiber (CNF) formed a layered-net structure.					
36972825	1	29	theme	nanofiber/Mg	318:329	arg1	CCMg					337:340	CCMg	337:340	CCMg	337:340	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	1	29	theme	nanofiber/Mg	318:329	arg1	agent					289:293	a high-efficient-sewage treatment agent	255:293	a high-efficient-sewage treatment agent	255:293	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	1	29	theme	nanofiber/Mg	318:329	arg1	OH					331:332	carbon dots/cellulose nanofiber/Mg(OH)2	296:334	carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg)	296:341	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	2	30	theme	cellulose	439:447	arg1	CNF					460:462	CNF	460:462	CNF	460:462	A variety of characterizations show that cellulose nanofiber (CNF) formed a layered-net structure.					
36972825	2	30	theme	cellulose	439:447	arg1	nanofiber					449:457	cellulose nanofiber	439:457	cellulose nanofiber (CNF)	439:463	A variety of characterizations show that cellulose nanofiber (CNF) formed a layered-net structure.					
36972825	1	31	theme	simple	370:375	arg1	method					390:395	a simple hydrothermal method	368:395	a simple hydrothermal method	368:395	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	3	32	theme	Mg	507:508	arg1	flakes					515:520	Hexagonal Mg(OH)2 flakes	497:520	Hexagonal Mg(OH)2 flakes of about 100 nm	497:536	Hexagonal Mg(OH)2 flakes of about 100 nm has been attached on CNF.					
36972825	10	33	theme	Cd2+/Cu2+	1078:1086	arg1	ions					1088:1091	Cd2+/Cu2+ ions	1078:1091	Cd2+/Cu2+ ions	1078:1091	Practically, Cd2+/Cu2+ ions were immobilized by CNF due to the space confinement effect.					
36972825	11	34	theme	facile	1170:1175	arg1	separation					1177:1186	facile separation	1170:1186	facile separation	1170:1186	It achieves the facile separation and recovery of HMIs from the sewage, and more importantly, eliminates the risk of secondary contamination.					
36972825	1	35	theme	hydrothermal	377:388	arg1	method					390:395	a simple hydrothermal method	368:395	a simple hydrothermal method	368:395	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	0	36	theme	carbon	41:46	arg1	effect					12:17	Confinement effect	0:17	Confinement effect of network-structured carbon	0:46	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	11	37	from	sewage	1218:1223	arg1	recovery					1192:1199	recovery	1192:1199	recovery	1192:1199	It achieves the facile separation and recovery of HMIs from the sewage, and more importantly, eliminates the risk of secondary contamination.					
36972825	11	37	from	sewage	1218:1223	arg1	separation					1177:1186	facile separation	1170:1186	facile separation	1170:1186	It achieves the facile separation and recovery of HMIs from the sewage, and more importantly, eliminates the risk of secondary contamination.					
36972825	5	38	theme	extraordinary	660:672	arg1	feature					685:691	The extraordinary structural feature	656:691	The extraordinary structural feature	656:691	The extraordinary structural feature endows CCMg with high removal performance towards HMIs.					
36972825	11	39	dep	separation	1177:1186	arg1	the					1166:1168	the	1166:1168	the	1166:1168	It achieves the facile separation and recovery of HMIs from the sewage, and more importantly, eliminates the risk of secondary contamination.					
36972825	0	40	theme	nanocellulose/magnesium	63:85	arg1	hydroxide					87:95	nanocellulose/magnesium hydroxide	63:95	nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization	63:152	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	1	41	theme	sustainable	230:240	arg1	development					242:252	sustainable development	230:252	sustainable development	230:252	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	11	42	theme	HMIs	1204:1207	arg1	recovery					1192:1199	recovery	1192:1199	recovery	1192:1199	It achieves the facile separation and recovery of HMIs from the sewage, and more importantly, eliminates the risk of secondary contamination.					
36972825	11	42	theme	HMIs	1204:1207	arg1	separation					1177:1186	facile separation	1170:1186	facile separation	1170:1186	It achieves the facile separation and recovery of HMIs from the sewage, and more importantly, eliminates the risk of secondary contamination.					
36972825	1	43	theme	pollution	164:172	arg1	problem					174:180	pollution problem	164:180	pollution problem of heavy metal ions (HMIs)	164:207	To solve pollution problem of heavy metal ions (HMIs) and recover them for sustainable development, a high-efficient-sewage treatment agent, carbon dots/cellulose nanofiber/Mg(OH)2 (CCMg), has been fabricated via a simple hydrothermal method.					
36972825	7	44	theme	excellent	855:863	arg1	durability					865:874	excellent durability	855:874	excellent durability	855:874	The composite bears excellent durability in treating wastewater.					
36972825	3	45	theme	100 nm	531:536	arg1	flakes					515:520	Hexagonal Mg(OH)2 flakes	497:520	Hexagonal Mg(OH)2 flakes of about 100 nm	497:536	Hexagonal Mg(OH)2 flakes of about 100 nm has been attached on CNF.					
36972825	0	46	theme	enhanced	101:108	arg1	capture					127:133	capture	127:133	capture	127:133	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	0	46	theme	enhanced	101:108	arg1	ions					122:125	enhanced heavy metal ions capture and immobilization	101:152	enhanced heavy metal ions capture and immobilization	101:152	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	0	46	theme	enhanced	101:108	arg1	immobilization					139:152	immobilization	139:152	immobilization	139:152	Confinement effect of network-structured carbon dots/cellulose nanocellulose/magnesium hydroxide for enhanced heavy metal ions capture and immobilization.					
36972825	10	47	theme	space	1128:1132	arg1	effect					1146:1151	the space confinement effect	1124:1151	the space confinement effect	1124:1151	Practically, Cd2+/Cu2+ ions were immobilized by CNF due to the space confinement effect.					
36972825	5	48	theme	high	710:713	arg1	performance					723:733	high removal performance	710:733	high removal performance towards HMIs	710:746	The extraordinary structural feature endows CCMg with high removal performance towards HMIs.					
36972825	4	49	theme	Carbon	564:569	arg1	dots					571:574	Carbon dots	564:574	Carbon dots (CDs) around 10-20 nm in size	564:604	Carbon dots (CDs) around 10-20 nm in size were produced from CNF and distributed along CNF.					
36972825	4	49	theme	Carbon	564:569	arg1	CDs					577:579	CDs	577:579	CDs	577:579	Carbon dots (CDs) around 10-20 nm in size were produced from CNF and distributed along CNF.					
35395257	5	0	theme	Porous	787:792	arg1	aerogels					798:805	Porous CNF aerogels	787:805	Porous CNF aerogels with homogenously distributed 20-40 nm Cu nanoparticles	787:861	Porous CNF aerogels with homogenously distributed 20-40 nm Cu nanoparticles were obtained by adsorbing Cu2+ ions and chemically reducing them to Cu metal.					
35395257	8	1	theme	reaction	1215:1222	arg1	kapp					1239:1242	kapp	1239:1242	kapp	1239:1242	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	8	1	theme	reaction	1215:1222	arg1	constant					1229:1236	reaction rate constant	1215:1236	reaction rate constant (kapp)	1215:1243	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	9	2	theme	economical	1623:1632	arg1	catalysts					1634:1642	practical and economical catalysts	1609:1642	practical and economical catalysts	1609:1642	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	9	2	theme	economical	1623:1632	arg1	potential					1596:1604	strong potential	1589:1604	strong potential	1589:1604	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	0	3	with	aerogels	32:39	arg1	loading					81:87	a controllable copper nanoparticle loading	46:87	a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction	46:162	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.					
35395257	5	4	theme	Cu	932:933	arg1	metal					935:939	Cu metal	932:939	Cu metal	932:939	Porous CNF aerogels with homogenously distributed 20-40 nm Cu nanoparticles were obtained by adsorbing Cu2+ ions and chemically reducing them to Cu metal.					
35395257	3	5	from	application	564:574	arg1	treatment					594:602	4NP wastewater treatment	579:602	4NP wastewater treatment	579:602	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	9	6	theme	corresponding	1453:1465	arg1	reactions					1467:1475	the corresponding reactions	1449:1475	the corresponding reactions involving noble-metal catalysts	1449:1507	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	5	7	theme	CNF	794:796	arg1	aerogels					798:805	Porous CNF aerogels	787:805	Porous CNF aerogels with homogenously distributed 20-40 nm Cu nanoparticles	787:861	Porous CNF aerogels with homogenously distributed 20-40 nm Cu nanoparticles were obtained by adsorbing Cu2+ ions and chemically reducing them to Cu metal.					
35395257	6	8	used	used	992:995	arg2	analysis					978:985	ICP-OES analysis	970:985	ICP-OES analysis	970:985	The FTIR, XRD, SEM, XPS and ICP-OES analysis were used to confirm the in-situ formation of Cu nanoparticles.					
35395257	6	8	used	used	992:995	arg2	XRD					952:954	XRD	952:954	XRD	952:954	The FTIR, XRD, SEM, XPS and ICP-OES analysis were used to confirm the in-situ formation of Cu nanoparticles.					
35395257	6	8	used	used	992:995	arg2	FTIR					946:949	The FTIR	942:949	The FTIR	942:949	The FTIR, XRD, SEM, XPS and ICP-OES analysis were used to confirm the in-situ formation of Cu nanoparticles.					
35395257	6	8	used	used	992:995	arg2	XPS					962:964	XPS	962:964	XPS	962:964	The FTIR, XRD, SEM, XPS and ICP-OES analysis were used to confirm the in-situ formation of Cu nanoparticles.					
35395257	6	8	used	used	992:995	arg2	SEM					957:959	SEM	957:959	SEM	957:959	The FTIR, XRD, SEM, XPS and ICP-OES analysis were used to confirm the in-situ formation of Cu nanoparticles.					
35395257	3	9	theme	appropriate	529:539	arg1	aerogel					515:521	CNF/PEI-Cu aerogel	504:521	CNF/PEI-Cu aerogel	504:521	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	3	9	theme	appropriate	529:539	arg1	catalyst					541:548	an appropriate catalyst	526:548	an appropriate catalyst for practical application in 4NP wastewater treatment	526:602	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	9	10	theme	noble-metal	1487:1497	arg1	catalysts					1499:1507	noble-metal catalysts	1487:1507	noble-metal catalysts	1487:1507	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	2	11	theme	nanofibril	311:320	arg1	catalyst					349:356	a catalyst	347:356	a catalyst for degrading 4-nitrophenol (4NP) in the wastewater	347:408	Herein, we investigated Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels as a catalyst for degrading 4-nitrophenol (4NP) in the wastewater.					
35395257	2	11	theme	nanofibril	311:320	arg1	aerogels					335:342	Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels	278:342	Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels	278:342	Herein, we investigated Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels as a catalyst for degrading 4-nitrophenol (4NP) in the wastewater.					
35395257	7	12	theme	nanoparticles	1169:1181	arg1	loss					1154:1157	loss	1154:1157	loss of the Cu nanoparticles	1154:1181	In the presence of the CNF/PEI-Cu aerogels, 4NP was effectively reduced to 4-aminophenol (4AP) without loss of the Cu nanoparticles.					
35395257	9	13	theme	reactions	1467:1475	arg1	values					1439:1444	the Ea values	1432:1444	the Ea values of the corresponding reactions involving noble-metal catalysts	1432:1507	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	3	14	theme	4NP	579:581	arg1	treatment					594:602	4NP wastewater treatment	579:602	4NP wastewater treatment	579:602	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	2	15	theme	cellulose	301:309	arg1	catalyst					349:356	a catalyst	347:356	a catalyst for degrading 4-nitrophenol (4NP) in the wastewater	347:408	Herein, we investigated Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels as a catalyst for degrading 4-nitrophenol (4NP) in the wastewater.					
35395257	2	15	theme	cellulose	301:309	arg1	aerogels					335:342	Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels	278:342	Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels	278:342	Herein, we investigated Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels as a catalyst for degrading 4-nitrophenol (4NP) in the wastewater.					
35395257	8	16	theme	catalytic	1252:1260	arg1	reaction					1276:1283	the catalytic 4NP reduction reaction	1248:1283	the catalytic 4NP reduction reaction	1248:1283	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	0	17	theme	non-noble-metal	111:125	arg1	catalyst					127:134	a highly efficient non-noble-metal catalyst	92:134	a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction	92:162	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.					
35395257	2	18	theme	nanoparticle-loaded	281:299	arg1	catalyst					349:356	a catalyst	347:356	a catalyst for degrading 4-nitrophenol (4NP) in the wastewater	347:408	Herein, we investigated Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels as a catalyst for degrading 4-nitrophenol (4NP) in the wastewater.					
35395257	2	18	theme	nanoparticle-loaded	281:299	arg1	aerogels					335:342	Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels	278:342	Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels	278:342	Herein, we investigated Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels as a catalyst for degrading 4-nitrophenol (4NP) in the wastewater.					
35395257	4	19	theme	CNF	717:719	arg1	aerogel					721:727	a CNF aerogel	715:727	a CNF aerogel	715:727	Our strategy to improve the loading amount of homogeneously distributed Cu nanoparticles was to functionalize a CNF aerogel using polyethylene imine (PEI), which can bind Cu2+ ions.					
35395257	0	20	theme	efficient	101:109	arg1	catalyst					127:134	a highly efficient non-noble-metal catalyst	92:134	a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction	92:162	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.					
35395257	2	21	theme	Cu	278:279	arg1	catalyst					349:356	a catalyst	347:356	a catalyst for degrading 4-nitrophenol (4NP) in the wastewater	347:408	Herein, we investigated Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels as a catalyst for degrading 4-nitrophenol (4NP) in the wastewater.					
35395257	2	21	theme	Cu	278:279	arg1	aerogels					335:342	Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels	278:342	Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels	278:342	Herein, we investigated Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels as a catalyst for degrading 4-nitrophenol (4NP) in the wastewater.					
35395257	7	22	theme	Cu	1166:1167	arg1	nanoparticles					1169:1181	the Cu nanoparticles	1162:1181	the Cu nanoparticles	1162:1181	In the presence of the CNF/PEI-Cu aerogels, 4NP was effectively reduced to 4-aminophenol (4AP) without loss of the Cu nanoparticles.					
35395257	0	23	theme	porous	4:9	arg1	aerogels					32:39	3-D porous cellulose nanofibril aerogels	0:39	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.	0:163	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.					
35395257	6	24	theme	Cu	1033:1034	arg1	nanoparticles					1036:1048	Cu nanoparticles	1033:1048	Cu nanoparticles	1033:1048	The FTIR, XRD, SEM, XPS and ICP-OES analysis were used to confirm the in-situ formation of Cu nanoparticles.					
35395257	7	25	theme	CNF/PEI-Cu	1074:1083	arg1	aerogels					1085:1092	the CNF/PEI-Cu aerogels	1070:1092	the CNF/PEI-Cu aerogels	1070:1092	In the presence of the CNF/PEI-Cu aerogels, 4NP was effectively reduced to 4-aminophenol (4AP) without loss of the Cu nanoparticles.					
35395257	4	26	theme	Cu2+	776:779	arg1	ions					781:784	Cu2+ ions	776:784	Cu2+ ions	776:784	Our strategy to improve the loading amount of homogeneously distributed Cu nanoparticles was to functionalize a CNF aerogel using polyethylene imine (PEI), which can bind Cu2+ ions.					
35395257	3	27	theme	low-cost	433:440	arg1	material					451:458	Non-noble metal based low-cost catalyst material	411:458	Non-noble metal based low-cost catalyst material	411:458	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	0	28	theme	3-D	0:2	arg1	aerogels					32:39	3-D porous cellulose nanofibril aerogels	0:39	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.	0:163	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.					
35395257	7	29	theme	aerogels	1085:1092	arg1	presence					1058:1065	the presence	1054:1065	the presence of the CNF/PEI-Cu aerogels	1054:1092	In the presence of the CNF/PEI-Cu aerogels, 4NP was effectively reduced to 4-aminophenol (4AP) without loss of the Cu nanoparticles.					
35395257	1	30	theme	toxic	194:198	arg1	Nitrophenols					165:176	Nitrophenols	165:176	Nitrophenols(NPs)	165:181	Nitrophenols(NPs) are highly toxic compounds that occur in various industrial effluents.					
35395257	1	30	theme	toxic	194:198	arg1	compounds					200:208	highly toxic compounds	187:208	highly toxic compounds that occur in various industrial effluents	187:251	Nitrophenols(NPs) are highly toxic compounds that occur in various industrial effluents.					
35395257	0	31	theme	nanofibril	21:30	arg1	aerogels					32:39	3-D porous cellulose nanofibril aerogels	0:39	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.	0:163	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.					
35395257	5	32	theme	Cu	846:847	arg1	nanoparticles					849:861	homogenously distributed 20-40 nm Cu nanoparticles	812:861	homogenously distributed 20-40 nm Cu nanoparticles	812:861	Porous CNF aerogels with homogenously distributed 20-40 nm Cu nanoparticles were obtained by adsorbing Cu2+ ions and chemically reducing them to Cu metal.					
35395257	3	33	theme	wastewater	583:592	arg1	treatment					594:602	4NP wastewater treatment	579:602	4NP wastewater treatment	579:602	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	3	34	theme	preparation	480:490	arg1	method					492:497	easily scalable preparation method	464:497	easily scalable preparation method	464:497	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	0	35	theme	cellulose	11:19	arg1	aerogels					32:39	3-D porous cellulose nanofibril aerogels	0:39	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.	0:163	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.					
35395257	3	36	theme	practical	554:562	arg1	application					564:574	practical application	554:574	practical application in 4NP wastewater treatment	554:602	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	4	37	theme	polyethylene	735:746	arg1	PEI					755:757	PEI	755:757	PEI	755:757	Our strategy to improve the loading amount of homogeneously distributed Cu nanoparticles was to functionalize a CNF aerogel using polyethylene imine (PEI), which can bind Cu2+ ions.					
35395257	4	37	theme	polyethylene	735:746	arg1	imine					748:752	polyethylene imine	735:752	polyethylene imine (PEI)	735:758	Our strategy to improve the loading amount of homogeneously distributed Cu nanoparticles was to functionalize a CNF aerogel using polyethylene imine (PEI), which can bind Cu2+ ions.					
35395257	4	38	theme	distributed	665:675	arg1	nanoparticles					680:692	homogeneously distributed Cu nanoparticles	651:692	homogeneously distributed Cu nanoparticles	651:692	Our strategy to improve the loading amount of homogeneously distributed Cu nanoparticles was to functionalize a CNF aerogel using polyethylene imine (PEI), which can bind Cu2+ ions.					
35395257	3	39	theme	metal	421:425	arg1	material					451:458	Non-noble metal based low-cost catalyst material	411:458	Non-noble metal based low-cost catalyst material	411:458	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	9	40	theme	Ea	1436:1437	arg1	values					1439:1444	the Ea values	1432:1444	the Ea values of the corresponding reactions involving noble-metal catalysts	1432:1507	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	9	41	theme	present	1570:1576	arg1	study					1578:1582	the present study	1566:1582	the present study	1566:1582	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	3	42	theme	catalyst	442:449	arg1	material					451:458	Non-noble metal based low-cost catalyst material	411:458	Non-noble metal based low-cost catalyst material	411:458	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	6	43	theme	nanoparticles	1036:1048	arg1	formation					1020:1028	the in-situ formation	1008:1028	the in-situ formation of Cu nanoparticles	1008:1048	The FTIR, XRD, SEM, XPS and ICP-OES analysis were used to confirm the in-situ formation of Cu nanoparticles.					
35395257	3	44	theme	based	427:431	arg1	material					451:458	Non-noble metal based low-cost catalyst material	411:458	Non-noble metal based low-cost catalyst material	411:458	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	0	45	theme	4-nitrophenol	140:152	arg1	reduction					154:162	4-nitrophenol reduction	140:162	4-nitrophenol reduction	140:162	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.					
35395257	8	46	theme	kapp = 0.770	1359:1370	arg1	min-1					1372:1376	kapp = 0.770 min-1	1359:1376	kapp = 0.770 min-1	1359:1376	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	5	47	theme	Cu2+	890:893	arg1	ions					895:898	Cu2+ ions	890:898	Cu2+ ions	890:898	Porous CNF aerogels with homogenously distributed 20-40 nm Cu nanoparticles were obtained by adsorbing Cu2+ ions and chemically reducing them to Cu metal.					
35395257	3	48	theme	scalable	471:478	arg1	method					492:497	easily scalable preparation method	464:497	easily scalable preparation method	464:497	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	0	49	theme	controllable	48:59	arg1	loading					81:87	a controllable copper nanoparticle loading	46:87	a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction	46:162	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.					
35395257	5	50	theme	distributed	825:835	arg1	nanoparticles					849:861	homogenously distributed 20-40 nm Cu nanoparticles	812:861	homogenously distributed 20-40 nm Cu nanoparticles	812:861	Porous CNF aerogels with homogenously distributed 20-40 nm Cu nanoparticles were obtained by adsorbing Cu2+ ions and chemically reducing them to Cu metal.					
35395257	6	51	theme	ICP-OES	970:976	arg1	analysis					978:985	ICP-OES analysis	970:985	ICP-OES analysis	970:985	The FTIR, XRD, SEM, XPS and ICP-OES analysis were used to confirm the in-situ formation of Cu nanoparticles.					
35395257	8	52	theme	reduction	1266:1274	arg1	reaction					1276:1283	the catalytic 4NP reduction reaction	1248:1283	the catalytic 4NP reduction reaction	1248:1283	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	3	53	theme	Non-noble	411:419	arg1	material					451:458	Non-noble metal based low-cost catalyst material	411:458	Non-noble metal based low-cost catalyst material	411:458	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	9	54	theme	strong	1589:1594	arg1	catalysts					1634:1642	practical and economical catalysts	1609:1642	practical and economical catalysts	1609:1642	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	9	54	theme	strong	1589:1594	arg1	potential					1596:1604	strong potential	1589:1604	strong potential	1589:1604	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	8	55	theme	activation	1188:1197	arg1	energy					1199:1204	The activation energy	1184:1204	The activation energy (Ea)	1184:1209	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	8	55	theme	activation	1188:1197	arg1	Ea = 39.56 kJ mol-1					1335:1353	Ea = 39.56 kJ mol-1	1335:1353	Ea = 39.56 kJ mol-1	1335:1353	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	8	55	theme	activation	1188:1197	arg1	Ea					1207:1208	Ea	1207:1208	Ea	1207:1208	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	9	56	contain	have	1584:1587	arg2	catalysts					1634:1642	practical and economical catalysts	1609:1642	practical and economical catalysts	1609:1642	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	9	56	contain	have	1584:1587	arg2	potential					1596:1604	strong potential	1589:1604	strong potential	1589:1604	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	9	56	contain	have	1584:1587	arg1	aerogels					1544:1551	the CNF/PEI-Cu aerogels	1529:1551	the CNF/PEI-Cu aerogels developed in the present study	1529:1582	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	8	57	theme	rate	1224:1227	arg1	kapp					1239:1242	kapp	1239:1242	kapp	1239:1242	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	8	57	theme	rate	1224:1227	arg1	constant					1229:1236	reaction rate constant	1215:1236	reaction rate constant (kapp)	1215:1243	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	8	58	theme	4NP	1262:1264	arg1	reaction					1276:1283	the catalytic 4NP reduction reaction	1248:1283	the catalytic 4NP reduction reaction	1248:1283	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	2	59	theme	CNF/PEI-Cu	323:332	arg1	catalyst					349:356	a catalyst	347:356	a catalyst for degrading 4-nitrophenol (4NP) in the wastewater	347:408	Herein, we investigated Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels as a catalyst for degrading 4-nitrophenol (4NP) in the wastewater.					
35395257	2	59	theme	CNF/PEI-Cu	323:332	arg1	aerogels					335:342	Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels	278:342	Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels	278:342	Herein, we investigated Cu nanoparticle-loaded cellulose nanofibril (CNF/PEI-Cu) aerogels as a catalyst for degrading 4-nitrophenol (4NP) in the wastewater.					
35395257	1	60	theme	various	224:230	arg1	effluents					243:251	various industrial effluents	224:251	various industrial effluents	224:251	Nitrophenols(NPs) are highly toxic compounds that occur in various industrial effluents.					
35395257	9	61	theme	CNF/PEI-Cu	1533:1542	arg1	aerogels					1544:1551	the CNF/PEI-Cu aerogels	1529:1551	the CNF/PEI-Cu aerogels developed in the present study	1529:1582	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	3	62	theme	CNF/PEI-Cu	504:513	arg1	aerogel					515:521	CNF/PEI-Cu aerogel	504:521	CNF/PEI-Cu aerogel	504:521	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	3	62	theme	CNF/PEI-Cu	504:513	arg1	catalyst					541:548	an appropriate catalyst	526:548	an appropriate catalyst for practical application in 4NP wastewater treatment	526:602	Non-noble metal based low-cost catalyst material and easily scalable preparation method make CNF/PEI-Cu aerogel as an appropriate catalyst for practical application in 4NP wastewater treatment.					
35395257	8	63	theme	CNF/PEI2-Cu	1292:1302	arg1	aerogels					1304:1311	the CNF/PEI2-Cu aerogels	1288:1311	the CNF/PEI2-Cu aerogels	1288:1311	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	1	64	theme	industrial	232:241	arg1	effluents					243:251	various industrial effluents	224:251	various industrial effluents	224:251	Nitrophenols(NPs) are highly toxic compounds that occur in various industrial effluents.					
35395257	0	65	theme	nanoparticle	68:79	arg1	loading					81:87	a controllable copper nanoparticle loading	46:87	a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction	46:162	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.					
35395257	4	66	theme	nanoparticles	680:692	arg1	nanoparticles					680:692	homogeneously distributed Cu nanoparticles	651:692	homogeneously distributed Cu nanoparticles	651:692	Our strategy to improve the loading amount of homogeneously distributed Cu nanoparticles was to functionalize a CNF aerogel using polyethylene imine (PEI), which can bind Cu2+ ions.					
35395257	4	66	theme	nanoparticles	680:692	arg1	amount					641:646	the loading amount	629:646	the loading amount of homogeneously distributed Cu nanoparticles	629:692	Our strategy to improve the loading amount of homogeneously distributed Cu nanoparticles was to functionalize a CNF aerogel using polyethylene imine (PEI), which can bind Cu2+ ions.					
35395257	9	67	theme	practical	1609:1617	arg1	catalysts					1634:1642	practical and economical catalysts	1609:1642	practical and economical catalysts	1609:1642	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	9	67	theme	practical	1609:1617	arg1	potential					1596:1604	strong potential	1589:1604	strong potential	1589:1604	The Ea is similar or even smaller than the Ea values of the corresponding reactions involving noble-metal catalysts, demonstrating that the CNF/PEI-Cu aerogels developed in the present study have strong potential as practical and economical catalysts.					
35395257	4	68	theme	Cu	677:678	arg1	nanoparticles					680:692	homogeneously distributed Cu nanoparticles	651:692	homogeneously distributed Cu nanoparticles	651:692	Our strategy to improve the loading amount of homogeneously distributed Cu nanoparticles was to functionalize a CNF aerogel using polyethylene imine (PEI), which can bind Cu2+ ions.					
35395257	8	69	theme	reaction	1276:1283	arg1	energy					1199:1204	The activation energy	1184:1204	The activation energy (Ea)	1184:1209	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	8	69	theme	reaction	1276:1283	arg1	Ea = 39.56 kJ mol-1					1335:1353	Ea = 39.56 kJ mol-1	1335:1353	Ea = 39.56 kJ mol-1	1335:1353	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	8	69	theme	reaction	1276:1283	arg1	kapp					1239:1242	kapp	1239:1242	kapp	1239:1242	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	8	69	theme	reaction	1276:1283	arg1	constant					1229:1236	reaction rate constant	1215:1236	reaction rate constant (kapp)	1215:1243	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	8	69	theme	reaction	1276:1283	arg1	Ea					1207:1208	Ea	1207:1208	Ea	1207:1208	The activation energy (Ea) and reaction rate constant (kapp) of the catalytic 4NP reduction reaction by the CNF/PEI2-Cu aerogels were calculated to be Ea = 39.56 kJ mol-1 and kapp = 0.770 min-1, respectively.					
35395257	5	70	with	aerogels	798:805	arg1	nanoparticles					849:861	homogenously distributed 20-40 nm Cu nanoparticles	812:861	homogenously distributed 20-40 nm Cu nanoparticles	812:861	Porous CNF aerogels with homogenously distributed 20-40 nm Cu nanoparticles were obtained by adsorbing Cu2+ ions and chemically reducing them to Cu metal.					
35395257	6	71	theme	in-situ	1012:1018	arg1	formation					1020:1028	the in-situ formation	1008:1028	the in-situ formation of Cu nanoparticles	1008:1048	The FTIR, XRD, SEM, XPS and ICP-OES analysis were used to confirm the in-situ formation of Cu nanoparticles.					
35395257	0	72	theme	copper	61:66	arg1	loading					81:87	a controllable copper nanoparticle loading	46:87	a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction	46:162	3-D porous cellulose nanofibril aerogels with a controllable copper nanoparticle loading as a highly efficient non-noble-metal catalyst for 4-nitrophenol reduction.					
35395257	4	73	theme	loading	633:639	arg1	nanoparticles					680:692	homogeneously distributed Cu nanoparticles	651:692	homogeneously distributed Cu nanoparticles	651:692	Our strategy to improve the loading amount of homogeneously distributed Cu nanoparticles was to functionalize a CNF aerogel using polyethylene imine (PEI), which can bind Cu2+ ions.					
35395257	4	73	theme	loading	633:639	arg1	amount					641:646	the loading amount	629:646	the loading amount of homogeneously distributed Cu nanoparticles	629:692	Our strategy to improve the loading amount of homogeneously distributed Cu nanoparticles was to functionalize a CNF aerogel using polyethylene imine (PEI), which can bind Cu2+ ions.					
35395257	5	74	theme	20-40 nm	837:844	arg1	nanoparticles					849:861	homogenously distributed 20-40 nm Cu nanoparticles	812:861	homogenously distributed 20-40 nm Cu nanoparticles	812:861	Porous CNF aerogels with homogenously distributed 20-40 nm Cu nanoparticles were obtained by adsorbing Cu2+ ions and chemically reducing them to Cu metal.					
36906381	3	0	with	media	613:617	arg1	concentrations					629:642	high concentrations	624:642	high concentrations of NaCl	624:650	Since glycerol and trehalose are synthesized in the cell from the same precursor (glucose), we hypothesised that, under heat shock conditions, xerophiles growing in media with high concentrations of glycerol may acquire greater thermotolerance than those grown in media with high concentrations of NaCl.					
36906381	7	1	with	medium	1509:1514	arg1	salt					1521:1524	salt	1521:1524	salt	1521:1524	However, after exposure to HS the fungus acquires greater thermotolerance in the medium with glycerol than in the medium with salt.					
36906381	4	2	theme	lipids	692:697	arg1	composition					668:678	the composition	664:678	the composition of membrane lipids and osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions	664:802	Therefore, the composition of membrane lipids and osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions was studied and the acquired thermotolerance was assessed.					
36906381	5	3	from	decrease	973:980	arg1	proportion					989:998	the proportion	985:998	the proportion of phosphatidylethanolamines	985:1027	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	0	4	theme	fungus	83:88	arg1	penicillioides					102:115	xerohalophilic fungus Aspergillus penicillioides	68:115	xerohalophilic fungus Aspergillus penicillioides	68:115	Acquired thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides under heat shock.					
36906381	8	5	theme	glycerol	1709:1716	arg1	interrelation					1558:1570	the interrelation	1554:1570	the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS	1554:1669	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	8	5	theme	glycerol	1709:1716	arg1	effect					1699:1704	the synergistic effect	1683:1704	the synergistic effect of glycerol and trehalose	1683:1730	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	4	6	theme	membrane	683:690	arg1	lipids					692:697	membrane lipids	683:697	membrane lipids	683:697	Therefore, the composition of membrane lipids and osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions was studied and the acquired thermotolerance was assessed.					
36906381	6	7	theme	dry	1383:1385	arg1	weight					1387:1392	dry weight	1383:1392	dry weight	1383:1392	In the mycelium trehalose level have increased in both media, but did not exceed 1% of dry weight.					
36906381	3	8	theme	high	525:528	arg1	concentrations					530:543	high concentrations	525:543	high concentrations of glycerol	525:555	Since glycerol and trehalose are synthesized in the cell from the same precursor (glucose), we hypothesised that, under heat shock conditions, xerophiles growing in media with high concentrations of glycerol may acquire greater thermotolerance than those grown in media with high concentrations of NaCl.					
36906381	3	9	theme	glycerol	548:555	arg1	concentrations					530:543	high concentrations	525:543	high concentrations of glycerol	525:555	Since glycerol and trehalose are synthesized in the cell from the same precursor (glucose), we hypothesised that, under heat shock conditions, xerophiles growing in media with high concentrations of glycerol may acquire greater thermotolerance than those grown in media with high concentrations of NaCl.					
36906381	0	10	theme	penicillioides	102:115	arg1	profiles					56:63	thermotolerance, membrane lipids and osmolytes profiles	9:63	thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides	9:115	Acquired thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides under heat shock.					
36906381	8	11	theme	trehalose	1722:1730	arg1	interrelation					1558:1570	the interrelation	1554:1570	the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS	1554:1669	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	8	11	theme	trehalose	1722:1730	arg1	effect					1699:1704	the synergistic effect	1683:1704	the synergistic effect of glycerol and trehalose	1683:1730	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	8	12	from	changes	1580:1586	arg1	lipids					1633:1638	membrane lipids	1624:1638	membrane lipids	1624:1638	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	8	12	from	changes	1580:1586	arg1	composition					1595:1605	the composition	1591:1605	the composition of osmolytes	1591:1618	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	8	13	theme	membrane	1624:1631	arg1	lipids					1633:1638	membrane lipids	1624:1638	membrane lipids	1624:1638	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	3	14	theme	heat	469:472	arg1	conditions					480:489	heat shock conditions	469:489	heat shock conditions	469:489	Since glycerol and trehalose are synthesized in the cell from the same precursor (glucose), we hypothesised that, under heat shock conditions, xerophiles growing in media with high concentrations of glycerol may acquire greater thermotolerance than those grown in media with high concentrations of NaCl.					
36906381	6	15	theme	mycelium	1303:1310	arg1	level					1322:1326	the mycelium trehalose level	1299:1326	the mycelium trehalose level	1299:1326	In the mycelium trehalose level have increased in both media, but did not exceed 1% of dry weight.					
36906381	4	16	theme	osmolytes	703:711	arg1	composition					668:678	the composition	664:678	the composition of membrane lipids and osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions	664:802	Therefore, the composition of membrane lipids and osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions was studied and the acquired thermotolerance was assessed.					
36906381	4	17	dep	fungus	720:725	arg1	Aspergillus					727:737	Aspergillus	727:737	Aspergillus	727:737	Therefore, the composition of membrane lipids and osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions was studied and the acquired thermotolerance was assessed.					
36906381	6	18	theme	weight	1387:1392	arg1	weight					1387:1392	dry weight	1383:1392	dry weight	1383:1392	In the mycelium trehalose level have increased in both media, but did not exceed 1% of dry weight.					
36906381	6	18	theme	weight	1387:1392	arg1	%					1378:1378	1%	1377:1378	1% of dry weight	1377:1392	In the mycelium trehalose level have increased in both media, but did not exceed 1% of dry weight.					
36906381	4	19	theme	different	768:776	arg1	media					778:782	2 different media	766:782	2 different media	766:782	Therefore, the composition of membrane lipids and osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions was studied and the acquired thermotolerance was assessed.					
36906381	1	20	theme	glycerol	181:188	arg1	glycerol					181:188	glycerol	181:188	glycerol	181:188	Xerophilic fungi accumulate a large amount of glycerol in the cytosol to counterbalance the external osmotic pressure.					
36906381	1	20	theme	glycerol	181:188	arg1	amount					171:176	a large amount	163:176	a large amount of glycerol	163:188	Xerophilic fungi accumulate a large amount of glycerol in the cytosol to counterbalance the external osmotic pressure.					
36906381	0	21	theme	heat	123:126	arg1	shock					128:132	heat shock	123:132	heat shock	123:132	Acquired thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides under heat shock.					
36906381	5	22	theme	glycerol	1097:1104	arg1	level					1088:1092	the level	1084:1092	the level of glycerol in the cytosol	1084:1119	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	3	23	with	media	514:518	arg1	concentrations					530:543	high concentrations	525:543	high concentrations of glycerol	525:555	Since glycerol and trehalose are synthesized in the cell from the same precursor (glucose), we hypothesised that, under heat shock conditions, xerophiles growing in media with high concentrations of glycerol may acquire greater thermotolerance than those grown in media with high concentrations of NaCl.					
36906381	5	24	from	increase	914:921	arg1	proportion					930:939	the proportion	926:939	the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines	926:1027	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	8	25	from	interrelation	1558:1570	arg1	response					1656:1663	the adaptive response	1643:1663	the adaptive response to HS	1643:1669	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	0	26	theme	thermotolerance	9:23	arg1	profiles					56:63	thermotolerance, membrane lipids and osmolytes profiles	9:63	thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides	9:115	Acquired thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides under heat shock.					
36906381	5	27	from	medium	1152:1157	arg1	insignificant					1224:1236	insignificant	1224:1236	insignificant	1224:1236	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	2	28	theme	thermoprotective	312:327	arg1	trehalose					338:346	a thermoprotective osmolyte trehalose	310:346	a thermoprotective osmolyte trehalose	310:346	But during heat shock (HS) majority of fungi accumulate a thermoprotective osmolyte trehalose.					
36906381	5	29	with	medium	1152:1157	arg1	glycerol					1164:1171	glycerol	1164:1171	glycerol	1164:1171	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	0	30	dep	fungus	83:88	arg1	Aspergillus					90:100	Aspergillus	90:100	Aspergillus	90:100	Acquired thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides under heat shock.					
36906381	8	31	theme	adaptive	1647:1654	arg1	response					1656:1663	the adaptive response	1643:1663	the adaptive response to HS	1643:1669	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	5	32	theme	salt-containing	888:902	arg1	medium					904:909	the salt-containing medium	884:909	the salt-containing medium	884:909	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	0	33	theme	membrane	26:33	arg1	lipids					35:40	membrane lipids	26:40	membrane lipids	26:40	Acquired thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides under heat shock.					
36906381	5	34	theme	acids	957:961	arg1	proportion					930:939	the proportion	926:939	the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines	926:1027	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	4	35	theme	fungus	720:725	arg1	penicillioides					739:752	the fungus Aspergillus penicillioides	716:752	the fungus Aspergillus penicillioides	716:752	Therefore, the composition of membrane lipids and osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions was studied and the acquired thermotolerance was assessed.					
36906381	5	36	from	insignificant	1224:1236	arg1	medium					1152:1157	the medium	1148:1157	the medium with glycerol	1148:1171	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	5	37	theme	lipids	1072:1077	arg1	composition					1048:1058	the composition	1044:1058	the composition of membrane lipids	1044:1077	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	2	38	theme	fungi	293:297	arg1	majority					281:288	heat shock (HS) majority	265:288	heat shock (HS) majority of fungi	265:297	But during heat shock (HS) majority of fungi accumulate a thermoprotective osmolyte trehalose.					
36906381	3	39	theme	same	415:418	arg1	glucose					431:437	glucose	431:437	glucose	431:437	Since glycerol and trehalose are synthesized in the cell from the same precursor (glucose), we hypothesised that, under heat shock conditions, xerophiles growing in media with high concentrations of glycerol may acquire greater thermotolerance than those grown in media with high concentrations of NaCl.					
36906381	3	39	theme	same	415:418	arg1	precursor					420:428	the same precursor	411:428	the same precursor (glucose)	411:438	Since glycerol and trehalose are synthesized in the cell from the same precursor (glucose), we hypothesised that, under heat shock conditions, xerophiles growing in media with high concentrations of glycerol may acquire greater thermotolerance than those grown in media with high concentrations of NaCl.					
36906381	5	40	from	changes	1174:1180	arg1	composition					1189:1199	the composition	1185:1199	the composition of membrane lipids	1185:1218	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	3	41	theme	NaCl	647:650	arg1	concentrations					629:642	high concentrations	624:642	high concentrations of NaCl	624:650	Since glycerol and trehalose are synthesized in the cell from the same precursor (glucose), we hypothesised that, under heat shock conditions, xerophiles growing in media with high concentrations of glycerol may acquire greater thermotolerance than those grown in media with high concentrations of NaCl.					
36906381	5	42	theme	phosphatidylethanolamines	1003:1027	arg1	proportion					989:998	the proportion	985:998	the proportion of phosphatidylethanolamines	985:1027	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	2	43	dep	But	254:256	arg1	accumulate					299:308	accumulate	299:308	accumulate a thermoprotective osmolyte trehalose	299:346	But during heat shock (HS) majority of fungi accumulate a thermoprotective osmolyte trehalose.					
36906381	0	44	theme	lipids	35:40	arg1	profiles					56:63	thermotolerance, membrane lipids and osmolytes profiles	9:63	thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides	9:115	Acquired thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides under heat shock.					
36906381	3	45	theme	high	624:627	arg1	concentrations					629:642	high concentrations	624:642	high concentrations of NaCl	624:650	Since glycerol and trehalose are synthesized in the cell from the same precursor (glucose), we hypothesised that, under heat shock conditions, xerophiles growing in media with high concentrations of glycerol may acquire greater thermotolerance than those grown in media with high concentrations of NaCl.					
36906381	5	46	from	level	1088:1092	arg1	cytosol					1113:1119	the cytosol	1109:1119	the cytosol	1109:1119	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	1	47	theme	external	227:234	arg1	pressure					244:251	the external osmotic pressure	223:251	the external osmotic pressure	223:251	Xerophilic fungi accumulate a large amount of glycerol in the cytosol to counterbalance the external osmotic pressure.					
36906381	7	48	theme	greater	1445:1451	arg1	thermotolerance					1453:1467	greater thermotolerance	1445:1467	greater thermotolerance	1445:1467	However, after exposure to HS the fungus acquires greater thermotolerance in the medium with glycerol than in the medium with salt.					
36906381	3	49	theme	greater	569:575	arg1	thermotolerance					577:591	greater thermotolerance	569:591	greater thermotolerance than those grown in media with high concentrations of NaCl	569:650	Since glycerol and trehalose are synthesized in the cell from the same precursor (glucose), we hypothesised that, under heat shock conditions, xerophiles growing in media with high concentrations of glycerol may acquire greater thermotolerance than those grown in media with high concentrations of NaCl.					
36906381	7	50	with	medium	1476:1481	arg1	glycerol					1488:1495	glycerol	1488:1495	glycerol	1488:1495	However, after exposure to HS the fungus acquires greater thermotolerance in the medium with glycerol than in the medium with salt.					
36906381	3	51	theme	shock	474:478	arg1	conditions					480:489	heat shock conditions	469:489	heat shock conditions	469:489	Since glycerol and trehalose are synthesized in the cell from the same precursor (glucose), we hypothesised that, under heat shock conditions, xerophiles growing in media with high concentrations of glycerol may acquire greater thermotolerance than those grown in media with high concentrations of NaCl.					
36906381	0	52	theme	osmolytes	46:54	arg1	profiles					56:63	thermotolerance, membrane lipids and osmolytes profiles	9:63	thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides	9:115	Acquired thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides under heat shock.					
36906381	8	53	theme	osmolytes	1610:1618	arg1	lipids					1633:1638	membrane lipids	1624:1638	membrane lipids	1624:1638	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	8	53	theme	osmolytes	1610:1618	arg1	composition					1595:1605	the composition	1591:1605	the composition of osmolytes	1591:1618	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	4	54	theme	acquired	824:831	arg1	thermotolerance					833:847	the acquired thermotolerance	820:847	the acquired thermotolerance	820:847	Therefore, the composition of membrane lipids and osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions was studied and the acquired thermotolerance was assessed.					
36906381	1	55	theme	Xerophilic	135:144	arg1	fungi					146:150	Xerophilic fungi	135:150	Xerophilic fungi	135:150	Xerophilic fungi accumulate a large amount of glycerol in the cytosol to counterbalance the external osmotic pressure.					
36906381	8	56	theme	synergistic	1687:1697	arg1	effect					1699:1704	the synergistic effect	1683:1704	the synergistic effect of glycerol and trehalose	1683:1730	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	5	57	theme	glycerol	1255:1262	arg1	level					1246:1250	the level	1242:1250	the level of glycerol	1242:1262	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	1	58	theme	osmotic	236:242	arg1	pressure					244:251	the external osmotic pressure	223:251	the external osmotic pressure	223:251	Xerophilic fungi accumulate a large amount of glycerol in the cytosol to counterbalance the external osmotic pressure.					
36906381	2	59	theme	shock	270:274	arg1	majority					281:288	heat shock (HS) majority	265:288	heat shock (HS) majority of fungi	265:297	But during heat shock (HS) majority of fungi accumulate a thermoprotective osmolyte trehalose.					
36906381	4	60	theme	penicillioides	739:752	arg1	osmolytes					703:711	osmolytes	703:711	osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions	703:802	Therefore, the composition of membrane lipids and osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions was studied and the acquired thermotolerance was assessed.					
36906381	4	60	theme	penicillioides	739:752	arg1	lipids					692:697	membrane lipids	683:697	membrane lipids	683:697	Therefore, the composition of membrane lipids and osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions was studied and the acquired thermotolerance was assessed.					
36906381	2	61	theme	osmolyte	329:336	arg1	trehalose					338:346	a thermoprotective osmolyte trehalose	310:346	a thermoprotective osmolyte trehalose	310:346	But during heat shock (HS) majority of fungi accumulate a thermoprotective osmolyte trehalose.					
36906381	6	62	theme	trehalose	1312:1320	arg1	level					1322:1326	the mycelium trehalose level	1299:1326	the mycelium trehalose level	1299:1326	In the mycelium trehalose level have increased in both media, but did not exceed 1% of dry weight.					
36906381	5	63	theme	membrane	1204:1211	arg1	lipids					1213:1218	membrane lipids	1204:1218	membrane lipids	1204:1218	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	0	64	theme	xerohalophilic	68:81	arg1	penicillioides					102:115	xerohalophilic fungus Aspergillus penicillioides	68:115	xerohalophilic fungus Aspergillus penicillioides	68:115	Acquired thermotolerance, membrane lipids and osmolytes profiles of xerohalophilic fungus Aspergillus penicillioides under heat shock.					
36906381	5	65	theme	membrane	1063:1070	arg1	lipids					1072:1077	membrane lipids	1063:1077	membrane lipids	1063:1077	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	2	66	theme	heat	265:268	arg1	HS					277:278	HS	277:278	HS	277:278	But during heat shock (HS) majority of fungi accumulate a thermoprotective osmolyte trehalose.					
36906381	2	66	theme	heat	265:268	arg1	shock					270:274	heat shock	265:274	heat shock (HS) majority of fungi	265:297	But during heat shock (HS) majority of fungi accumulate a thermoprotective osmolyte trehalose.					
36906381	8	67	from	effect	1699:1704	arg1	response					1656:1663	the adaptive response	1643:1663	the adaptive response to HS	1643:1669	The data obtained indicate the interrelation between changes in the composition of osmolytes and membrane lipids in the adaptive response to HS, as well as the synergistic effect of glycerol and trehalose.					
36906381	5	68	theme	phosphatidic	944:955	arg1	acids					957:961	phosphatidic acids	944:961	phosphatidic acids	944:961	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	5	69	theme	lipids	1213:1218	arg1	composition					1189:1199	the composition	1185:1199	the composition of membrane lipids	1185:1218	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	4	70	theme	HS	790:791	arg1	conditions					793:802	HS conditions	790:802	HS conditions	790:802	Therefore, the composition of membrane lipids and osmolytes of the fungus Aspergillus penicillioides, growing in 2 different media under HS conditions was studied and the acquired thermotolerance was assessed.					
36906381	1	71	theme	large	165:169	arg1	glycerol					181:188	glycerol	181:188	glycerol	181:188	Xerophilic fungi accumulate a large amount of glycerol in the cytosol to counterbalance the external osmotic pressure.					
36906381	1	71	theme	large	165:169	arg1	amount					171:176	a large amount	163:176	a large amount of glycerol	163:188	Xerophilic fungi accumulate a large amount of glycerol in the cytosol to counterbalance the external osmotic pressure.					
36906381	5	72	located	observed	1032:1039	arg2	increase					914:921	an increase	911:921	an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines	911:1027	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	5	72	located	observed	1032:1039	arg1	composition					1048:1058	the composition	1044:1058	the composition of membrane lipids	1044:1077	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
36906381	5	72	located	observed	1032:1039	arg1	medium					904:909	the salt-containing medium	884:909	the salt-containing medium	884:909	It was found that in the salt-containing medium an increase in the proportion of phosphatidic acids against a decrease in the proportion of phosphatidylethanolamines is observed in the composition of membrane lipids, and the level of glycerol in the cytosol decreases 6-fold, while in the medium with glycerol, changes in the composition of membrane lipids are insignificant and the level of glycerol is reduced by no more than 30%.					
35500534	10	0	theme	starch-degrading	1577:1592	arg1	subfamilies					1594:1604	starch-degrading subfamilies	1577:1604	starch-degrading subfamilies	1577:1604	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	11	1	theme	starch	2021:2026	arg1	metabolism					2028:2037	resistant starch metabolism	2011:2037	resistant starch metabolism	2011:2037	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	3	2	theme	metabolites	533:543	arg1	diversity					569:577	diversity	569:577	diversity	569:577	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	3	2	theme	metabolites	533:543	arg1	composition					553:563	composition	553:563	composition	553:563	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	3	2	theme	metabolites	533:543	arg1	abundances					478:487	relative abundances	469:487	relative abundances	469:487	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	3	2	theme	metabolites	533:543	arg1	scores					502:507	turnover scores	493:507	turnover scores	493:507	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	9	3	theme	MS	1282:1283	arg1	participants					1285:1296	MS participants	1282:1296	MS participants	1282:1296	MS participants had a greater potential to metabolize lipopolysaccharides than controls (score difference=1.6E-04, p = 0.034) but lower potential to metabolize peptidoglycan molecules and starch (score differences<2.2E-02, p<0.040).					
35500534	2	4	dep	METHODS	231:237	arg1	included					242:249	included	242:249	included 17 MS participants and 20 controls similar for sex, age, race, and stool consistency from the Canadian Paediatric Demyelinating Disease Network study	242:399	METHODS We included 17 MS participants and 20 controls similar for sex, age, race, and stool consistency from the Canadian Paediatric Demyelinating Disease Network study.					
35500534	3	5	theme	individual	512:521	arg1	metabolites					533:543	individual microbial metabolites	512:543	individual microbial metabolites	512:543	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	3	6	link	Stool-derived	402:414	arg1	abundances					436:445	Stool-derived gut metagenome gene abundances	402:445	Stool-derived gut metagenome gene abundances	402:445	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	11	7	dep	metabolism	2028:2037	arg1	lower					2005:2009	lower	2005:2009	lower	2005:2009	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	10	8	theme	relative	1664:1671	arg1	participants					1634:1645	MS participants versus controls	1631:1661	participants	1634:1645	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	10	8	theme	relative	1664:1671	arg1	differences					1683:1693	relative abundance differences >-0.34	1664:1700	relative abundance differences >-0.34	1664:1700	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	9	9	theme	greater	1304:1310	arg1	potential					1312:1320	a greater potential	1302:1320	a greater potential to metabolize lipopolysaccharides	1302:1354	MS participants had a greater potential to metabolize lipopolysaccharides than controls (score difference=1.6E-04, p = 0.034) but lower potential to metabolize peptidoglycan molecules and starch (score differences<2.2E-02, p<0.040).					
35500534	11	10	theme	lipopolysaccharide	1971:1988	arg1	molecules					1990:1998	lipopolysaccharide molecules	1971:1998	lipopolysaccharide molecules	1971:1998	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	2	11	theme	Paediatric	354:363	arg1	study					395:399	the Canadian Paediatric Demyelinating Disease Network study	341:399	the Canadian Paediatric Demyelinating Disease Network study	341:399	METHODS We included 17 MS participants and 20 controls similar for sex, age, race, and stool consistency from the Canadian Paediatric Demyelinating Disease Network study.					
35500534	5	12	theme	sample	877:882	arg1	IQR=2.0/1.4					913:923	IQR=2.0/1.4	913:923	IQR=2.0/1.4	913:923	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	5	12	theme	sample	877:882	arg1	years					906:910	stool sample procurement=16.9/15.8 years	871:910	stool sample procurement=16.9/15.8 years (IQR=2.0/1.4)	871:924	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	3	13	theme	enzymes	602:608	arg1	diversity					569:577	diversity	569:577	diversity	569:577	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	3	13	theme	enzymes	602:608	arg1	composition					553:563	composition	553:563	composition	553:563	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	3	13	theme	enzymes	602:608	arg1	abundances					478:487	relative abundances	469:487	relative abundances	469:487	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	3	13	theme	enzymes	602:608	arg1	scores					502:507	turnover scores	493:507	turnover scores	493:507	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	5	14	theme	symptom	806:812	arg1	years					825:829	MS symptom onset=16.1 years	803:829	MS symptom onset=16.1 years (interquartile range [IQR]=1.7)	803:861	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	5	14	theme	symptom	806:812	arg1	range					846:850	interquartile range [IQR]=1.7	832:860	interquartile range [IQR]=1.7	832:860	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	11	15	contain	had	1861:1863	arg1	participants					1848:1859	CONCLUSION Paediatric-onset MS participants	1817:1859	CONCLUSION Paediatric-onset MS participants	1817:1859	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	11	15	contain	had	1861:1863	arg2	potential					1909:1917	an altered gut microbiome-related metabolic potential	1865:1917	an altered gut microbiome-related metabolic potential	1865:1917	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	9	16	dep	potential	1418:1426	arg1	lower					1412:1416	lower	1412:1416	lower	1412:1416	MS participants had a greater potential to metabolize lipopolysaccharides than controls (score difference=1.6E-04, p = 0.034) but lower potential to metabolize peptidoglycan molecules and starch (score differences<2.2E-02, p<0.040).					
35500534	11	17	theme	MS	1845:1846	arg1	participants					1848:1859	CONCLUSION Paediatric-onset MS participants	1817:1859	CONCLUSION Paediatric-onset MS participants	1817:1859	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	4	18	theme	disease-modifying	710:726	arg1	DMD					734:736	DMD	734:736	DMD	734:736	MS participants and controls were compared using the Wilcoxon rank-sum test, as were the disease-modifying drug (DMD) exposed and naïve MS participants.					
35500534	4	18	theme	disease-modifying	710:726	arg1	drug					728:731	disease-modifying drug	710:731	disease-modifying drug (DMD)	710:737	MS participants and controls were compared using the Wilcoxon rank-sum test, as were the disease-modifying drug (DMD) exposed and naïve MS participants.					
35500534	11	19	theme	microbiome-related	1880:1897	arg1	potential					1909:1917	an altered gut microbiome-related metabolic potential	1865:1917	an altered gut microbiome-related metabolic potential	1865:1917	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	6	20	theme	MS	966:967	arg1	participants					981:992	Most MS and control participants	961:992	Most MS and control participants	961:992	Most MS and control participants were girls (80-82%).					
35500534	4	21	theme	rank-sum	683:690	arg1	test					692:695	the Wilcoxon rank-sum test	670:695	the Wilcoxon rank-sum test	670:695	MS participants and controls were compared using the Wilcoxon rank-sum test, as were the disease-modifying drug (DMD) exposed and naïve MS participants.					
35500534	0	22	theme	gut	67:69	arg1	potential					14:22	The metabolic potential	0:22	The metabolic potential of the paediatric-onset multiple sclerosis gut	0:69	The metabolic potential of the paediatric-onset multiple sclerosis gut microbiome.					
35500534	11	23	theme	altered	1868:1874	arg1	potential					1909:1917	an altered gut microbiome-related metabolic potential	1865:1917	an altered gut microbiome-related metabolic potential	1865:1917	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	1	24	theme	gut	135:137	arg1	potential					162:170	the gut microbiome's metabolic potential	131:170	the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years)	131:228	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	4	25	dep	exposed	739:745	arg1	DMD					734:736	DMD	734:736	DMD	734:736	MS participants and controls were compared using the Wilcoxon rank-sum test, as were the disease-modifying drug (DMD) exposed and naïve MS participants.					
35500534	4	25	dep	exposed	739:745	arg1	drug					728:731	disease-modifying drug	710:731	disease-modifying drug (DMD)	710:737	MS participants and controls were compared using the Wilcoxon rank-sum test, as were the disease-modifying drug (DMD) exposed and naïve MS participants.					
35500534	9	26	contain	had	1298:1300	arg1	participants					1285:1296	MS participants	1282:1296	MS participants	1282:1296	MS participants had a greater potential to metabolize lipopolysaccharides than controls (score difference=1.6E-04, p = 0.034) but lower potential to metabolize peptidoglycan molecules and starch (score differences<2.2E-02, p<0.040).					
35500534	9	26	contain	had	1298:1300	arg2	potential					1312:1320	a greater potential	1302:1320	a greater potential to metabolize lipopolysaccharides	1302:1354	MS participants had a greater potential to metabolize lipopolysaccharides than controls (score difference=1.6E-04, p = 0.034) but lower potential to metabolize peptidoglycan molecules and starch (score differences<2.2E-02, p<0.040).					
35500534	8	27	theme	metabolites	1196:1206	arg1	abundance					1183:1191	the relative abundance	1170:1191	the relative abundance of metabolites	1170:1206	While the relative abundance of metabolites did not differ between MS participants and controls, turnover scores did.					
35500534	7	28	dep	beta-interferon	1121:1135	arg1	to					1118:1119	to	1118:1119	to	1118:1119	Five (29%) of the MS participants had never been exposed to a DMD pre-stool sample and 12 (71%) had (7 to beta-interferon and 5 glatiramer acetate).					
35500534	11	29	theme	CONCLUSION	1817:1826	arg1	participants					1848:1859	CONCLUSION Paediatric-onset MS participants	1817:1859	CONCLUSION Paediatric-onset MS participants	1817:1859	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	4	30	theme	MS	757:758	arg1	participants					760:771	the disease-modifying drug (DMD) exposed and naïve MS participants	706:771	the disease-modifying drug (DMD) exposed and naïve MS participants	706:771	MS participants and controls were compared using the Wilcoxon rank-sum test, as were the disease-modifying drug (DMD) exposed and naïve MS participants.					
35500534	10	31	theme	CAZymes	1533:1539	arg1	diversity					1541:1549	CAZymes diversity	1533:1549	CAZymes diversity	1533:1549	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	7	32	theme	pre-stool	1081:1089	arg1	sample					1091:1096	a DMD pre-stool sample	1075:1096	a DMD pre-stool sample	1075:1096	Five (29%) of the MS participants had never been exposed to a DMD pre-stool sample and 12 (71%) had (7 to beta-interferon and 5 glatiramer acetate).					
35500534	9	33	theme	peptidoglycan	1442:1454	arg1	molecules					1456:1464	peptidoglycan molecules	1442:1464	peptidoglycan molecules	1442:1464	MS participants had a greater potential to metabolize lipopolysaccharides than controls (score difference=1.6E-04, p = 0.034) but lower potential to metabolize peptidoglycan molecules and starch (score differences<2.2E-02, p<0.040).					
35500534	2	34	theme	Network	387:393	arg1	study					395:399	the Canadian Paediatric Demyelinating Disease Network study	341:399	the Canadian Paediatric Demyelinating Disease Network study	341:399	METHODS We included 17 MS participants and 20 controls similar for sex, age, race, and stool consistency from the Canadian Paediatric Demyelinating Disease Network study.					
35500534	10	35	theme	MS	1631:1632	arg1	participants					1634:1645	MS participants versus controls	1631:1661	participants	1634:1645	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	10	35	theme	MS	1631:1632	arg1	differences					1683:1693	relative abundance differences >-0.34	1664:1700	relative abundance differences >-0.34	1664:1700	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	2	36	theme	Demyelinating	365:377	arg1	study					395:399	the Canadian Paediatric Demyelinating Disease Network study	341:399	the Canadian Paediatric Demyelinating Disease Network study	341:399	METHODS We included 17 MS participants and 20 controls similar for sex, age, race, and stool consistency from the Canadian Paediatric Demyelinating Disease Network study.					
35500534	3	37	theme	metagenome	420:429	arg1	abundances					436:445	Stool-derived gut metagenome gene abundances	402:445	Stool-derived gut metagenome gene abundances	402:445	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	6	38	theme	control	973:979	arg1	participants					981:992	Most MS and control participants	961:992	Most MS and control participants	961:992	Most MS and control participants were girls (80-82%).					
35500534	10	39	theme	DMD	1723:1725	arg1	verses					1735:1740	the DMD exposed verses	1719:1740	the DMD exposed verses DMD naïve MS participants	1719:1766	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	8	40	theme	turnover	1261:1268	arg1	scores					1270:1275	turnover scores	1261:1275	turnover scores	1261:1275	While the relative abundance of metabolites did not differ between MS participants and controls, turnover scores did.					
35500534	0	41	theme	multiple	48:55	arg1	sclerosis					57:65	the paediatric-onset multiple sclerosis	27:65	the paediatric-onset multiple sclerosis gut	27:69	The metabolic potential of the paediatric-onset multiple sclerosis gut microbiome.					
35500534	7	42	theme	5	1141:1141	arg1	glatiramer					1143:1152	glatiramer	1143:1152	glatiramer	1143:1152	Five (29%) of the MS participants had never been exposed to a DMD pre-stool sample and 12 (71%) had (7 to beta-interferon and 5 glatiramer acetate).					
35500534	5	43	from	years	906:910	arg1	RESULTS					774:780	RESULTS	774:780	RESULTS	774:780	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	1	44	theme	onset	213:217	arg1	years					223:227	symptom onset <18 years	205:227	symptom onset <18 years	205:227	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	1	44	theme	onset	213:217	arg1	patients					195:202	paediatric-onset MS patients	175:202	paediatric-onset MS patients (symptom onset <18 years)	175:228	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	10	45	theme	naïve	1746:1750	arg1	participants					1755:1766	naïve MS participants	1746:1766	naïve MS participants	1746:1766	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	9	46	dep	potential	1312:1320	arg1	metabolize					1325:1334	metabolize	1325:1334	to metabolize lipopolysaccharides	1322:1354	MS participants had a greater potential to metabolize lipopolysaccharides than controls (score difference=1.6E-04, p = 0.034) but lower potential to metabolize peptidoglycan molecules and starch (score differences<2.2E-02, p<0.040).					
35500534	3	47	theme	turnover	493:500	arg1	scores					502:507	turnover scores	493:507	turnover scores	493:507	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	0	48	theme	sclerosis	57:65	arg1	gut					67:69	the paediatric-onset multiple sclerosis gut	27:69	the paediatric-onset multiple sclerosis gut	27:69	The metabolic potential of the paediatric-onset multiple sclerosis gut microbiome.					
35500534	10	49	from	verses	1735:1740	arg1	underrepresented					1611:1626	underrepresented	1611:1626	underrepresented	1611:1626	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	10	50	theme	relative	1769:1776	arg1	abundance					1778:1786	relative abundance differences>-0.20	1769:1804	relative abundance differences>-0.20	1769:1804	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	10	51	dep	differ	1559:1564	arg1	p>0.050					1567:1573	p>0.050	1567:1573	p>0.050	1567:1573	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	3	52	theme	microbial	523:531	arg1	metabolites					533:543	individual microbial metabolites	512:543	individual microbial metabolites	512:543	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	5	53	from	years	825:829	arg1	RESULTS					774:780	RESULTS	774:780	RESULTS	774:780	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	11	54	theme	higher	1951:1956	arg1	breakdown					1958:1966	higher breakdown	1951:1966	higher breakdown of lipopolysaccharide molecules	1951:1998	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	2	55	theme	MS	254:255	arg1	participants					257:268	17 MS participants	251:268	17 MS participants	251:268	METHODS We included 17 MS participants and 20 controls similar for sex, age, race, and stool consistency from the Canadian Paediatric Demyelinating Disease Network study.					
35500534	1	56	theme	metabolic	152:160	arg1	potential					162:170	the gut microbiome's metabolic potential	131:170	the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years)	131:228	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	7	57	theme	participants	1036:1047	arg1	Five					1015:1018	Five	1015:1018	Five	1015:1018	Five (29%) of the MS participants had never been exposed to a DMD pre-stool sample and 12 (71%) had (7 to beta-interferon and 5 glatiramer acetate).					
35500534	7	57	theme	participants	1036:1047	arg1	%					1023:1023	29%	1021:1023	29%	1021:1023	Five (29%) of the MS participants had never been exposed to a DMD pre-stool sample and 12 (71%) had (7 to beta-interferon and 5 glatiramer acetate).					
35500534	7	57	theme	participants	1036:1047	arg1	participants					1036:1047	the MS participants	1029:1047	the MS participants	1029:1047	Five (29%) of the MS participants had never been exposed to a DMD pre-stool sample and 12 (71%) had (7 to beta-interferon and 5 glatiramer acetate).					
35500534	10	58	theme	abundance	1673:1681	arg1	participants					1634:1645	MS participants versus controls	1631:1661	participants	1634:1645	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	10	58	theme	abundance	1673:1681	arg1	differences					1683:1693	relative abundance differences >-0.34	1664:1700	relative abundance differences >-0.34	1664:1700	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	10	59	from	underrepresented	1611:1626	arg1	participants					1634:1645	MS participants versus controls	1631:1661	participants	1634:1645	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	10	59	from	underrepresented	1611:1626	arg1	verses					1735:1740	the DMD exposed verses	1719:1740	the DMD exposed verses DMD naïve MS participants	1719:1766	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	10	59	from	underrepresented	1611:1626	arg1	differences					1683:1693	relative abundance differences >-0.34	1664:1700	relative abundance differences >-0.34	1664:1700	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	1	60	theme	MS	192:193	arg1	years					223:227	symptom onset <18 years	205:227	symptom onset <18 years	205:227	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	1	60	theme	MS	192:193	arg1	patients					195:202	paediatric-onset MS patients	175:202	paediatric-onset MS patients (symptom onset <18 years)	175:228	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	2	61	theme	similar	286:292	arg1	participants					257:268	17 MS participants	251:268	17 MS participants	251:268	METHODS We included 17 MS participants and 20 controls similar for sex, age, race, and stool consistency from the Canadian Paediatric Demyelinating Disease Network study.					
35500534	11	62	theme	molecules	1990:1998	arg1	breakdown					1958:1966	higher breakdown	1951:1966	higher breakdown of lipopolysaccharide molecules	1951:1998	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	4	63	theme	MS	621:622	arg1	participants					624:635	MS participants	621:635	MS participants	621:635	MS participants and controls were compared using the Wilcoxon rank-sum test, as were the disease-modifying drug (DMD) exposed and naïve MS participants.					
35500534	3	64	theme	carbohydrate-active	582:600	arg1	CAZymes					611:617	CAZymes	611:617	CAZymes	611:617	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	3	64	theme	carbohydrate-active	582:600	arg1	enzymes					602:608	carbohydrate-active enzymes	582:608	carbohydrate-active enzymes (CAZymes)	582:618	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	1	65	theme	paediatric-onset	175:190	arg1	years					223:227	symptom onset <18 years	205:227	symptom onset <18 years	205:227	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	1	65	theme	paediatric-onset	175:190	arg1	patients					195:202	paediatric-onset MS patients	175:202	paediatric-onset MS patients (symptom onset <18 years)	175:228	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	5	66	theme	MS	803:804	arg1	years					825:829	MS symptom onset=16.1 years	803:829	MS symptom onset=16.1 years (interquartile range [IQR]=1.7)	803:861	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	5	66	theme	MS	803:804	arg1	range					846:850	interquartile range [IQR]=1.7	832:860	interquartile range [IQR]=1.7	832:860	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	11	67	theme	metabolic	1899:1907	arg1	potential					1909:1917	an altered gut microbiome-related metabolic potential	1865:1917	an altered gut microbiome-related metabolic potential	1865:1917	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	11	68	theme	Paediatric-onset	1828:1843	arg1	participants					1848:1859	CONCLUSION Paediatric-onset MS participants	1817:1859	CONCLUSION Paediatric-onset MS participants	1817:1859	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	1	69	theme	study	110:114	arg1	aim					98:100	The aim	94:100	The aim of this study	94:114	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	11	70	theme	resistant	2011:2019	arg1	metabolism					2028:2037	resistant starch metabolism	2011:2037	resistant starch metabolism	2011:2037	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	1	71	dep	BACKGROUND	83:92	arg1	was					116:118	was	116:118	was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years)	116:228	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	3	72	theme	Stool-derived	402:414	arg1	abundances					436:445	Stool-derived gut metagenome gene abundances	402:445	Stool-derived gut metagenome gene abundances	402:445	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	11	73	theme	gut	1876:1878	arg1	potential					1909:1917	an altered gut microbiome-related metabolic potential	1865:1917	an altered gut microbiome-related metabolic potential	1865:1917	CONCLUSION Paediatric-onset MS participants had an altered gut microbiome-related metabolic potential compared to controls, including higher breakdown of lipopolysaccharide molecules, but lower resistant starch metabolism.					
35500534	5	74	theme	interquartile	832:844	arg1	years					825:829	MS symptom onset=16.1 years	803:829	MS symptom onset=16.1 years (interquartile range [IQR]=1.7)	803:861	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	5	74	theme	interquartile	832:844	arg1	range					846:850	interquartile range [IQR]=1.7	832:860	interquartile range [IQR]=1.7	832:860	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	10	75	from	participants	1634:1645	arg1	underrepresented					1611:1626	underrepresented	1611:1626	underrepresented	1611:1626	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	6	76	theme	Most	961:964	arg1	participants					981:992	Most MS and control participants	961:992	Most MS and control participants	961:992	Most MS and control participants were girls (80-82%).					
35500534	4	77	theme	Wilcoxon	674:681	arg1	test					692:695	the Wilcoxon rank-sum test	670:695	the Wilcoxon rank-sum test	670:695	MS participants and controls were compared using the Wilcoxon rank-sum test, as were the disease-modifying drug (DMD) exposed and naïve MS participants.					
35500534	1	78	from	potential	162:170	arg1	years					223:227	symptom onset <18 years	205:227	symptom onset <18 years	205:227	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	1	78	from	potential	162:170	arg1	patients					195:202	paediatric-onset MS patients	175:202	paediatric-onset MS patients (symptom onset <18 years)	175:228	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	3	79	dep	composition	553:563	arg1	the					549:551	the	549:551	the	549:551	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	8	80	theme	relative	1174:1181	arg1	abundance					1183:1191	the relative abundance	1170:1191	the relative abundance of metabolites	1170:1206	While the relative abundance of metabolites did not differ between MS participants and controls, turnover scores did.					
35500534	4	81	theme	exposed	739:745	arg1	participants					760:771	the disease-modifying drug (DMD) exposed and naïve MS participants	706:771	the disease-modifying drug (DMD) exposed and naïve MS participants	706:771	MS participants and controls were compared using the Wilcoxon rank-sum test, as were the disease-modifying drug (DMD) exposed and naïve MS participants.					
35500534	7	82	theme	DMD	1077:1079	arg1	sample					1091:1096	a DMD pre-stool sample	1075:1096	a DMD pre-stool sample	1075:1096	Five (29%) of the MS participants had never been exposed to a DMD pre-stool sample and 12 (71%) had (7 to beta-interferon and 5 glatiramer acetate).					
35500534	10	83	dep	differences	1683:1693	arg1	p<0.040					1703:1709	p<0.040	1703:1709	p<0.040	1703:1709	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	10	83	dep	differences	1683:1693	arg1	>-0.34					1695:1700	>-0.34	1695:1700	>-0.34	1695:1700	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	4	84	theme	naïve	751:755	arg1	participants					760:771	the disease-modifying drug (DMD) exposed and naïve MS participants	706:771	the disease-modifying drug (DMD) exposed and naïve MS participants	706:771	MS participants and controls were compared using the Wilcoxon rank-sum test, as were the disease-modifying drug (DMD) exposed and naïve MS participants.					
35500534	3	85	theme	gut	416:418	arg1	abundances					436:445	Stool-derived gut metagenome gene abundances	402:445	Stool-derived gut metagenome gene abundances	402:445	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	5	86	theme	median	786:791	arg1	age					793:795	The median age	782:795	The median age(s)	782:798	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	5	86	theme	median	786:791	arg1	s					797:797	s	797:797	s	797:797	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	5	87	theme	stool	871:875	arg1	IQR=2.0/1.4					913:923	IQR=2.0/1.4	913:923	IQR=2.0/1.4	913:923	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	5	87	theme	stool	871:875	arg1	years					906:910	stool sample procurement=16.9/15.8 years	871:910	stool sample procurement=16.9/15.8 years (IQR=2.0/1.4)	871:924	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	2	88	dep	sex	298:300	arg1	consistency					324:334	consistency	324:334	consistency	324:334	METHODS We included 17 MS participants and 20 controls similar for sex, age, race, and stool consistency from the Canadian Paediatric Demyelinating Disease Network study.					
35500534	4	89	dep	participants	760:771	arg1	were					701:704	were	701:704	were	701:704	MS participants and controls were compared using the Wilcoxon rank-sum test, as were the disease-modifying drug (DMD) exposed and naïve MS participants.					
35500534	4	89	dep	participants	760:771	arg1	as					698:699	as	698:699	as	698:699	MS participants and controls were compared using the Wilcoxon rank-sum test, as were the disease-modifying drug (DMD) exposed and naïve MS participants.					
35500534	10	90	dep	underrepresented	1611:1626	arg1	abundance					1778:1786	relative abundance differences>-0.20	1769:1804	relative abundance differences>-0.20	1769:1804	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	8	91	theme	MS	1231:1232	arg1	participants					1234:1245	MS participants	1231:1245	MS participants	1231:1245	While the relative abundance of metabolites did not differ between MS participants and controls, turnover scores did.					
35500534	2	92	theme	Disease	379:385	arg1	study					395:399	the Canadian Paediatric Demyelinating Disease Network study	341:399	the Canadian Paediatric Demyelinating Disease Network study	341:399	METHODS We included 17 MS participants and 20 controls similar for sex, age, race, and stool consistency from the Canadian Paediatric Demyelinating Disease Network study.					
35500534	0	93	theme	paediatric-onset	31:46	arg1	sclerosis					57:65	the paediatric-onset multiple sclerosis	27:65	the paediatric-onset multiple sclerosis gut	27:69	The metabolic potential of the paediatric-onset multiple sclerosis gut microbiome.					
35500534	7	94	theme	glatiramer	1143:1152	arg1	acetate					1154:1160	5 glatiramer acetate	1141:1160	5 glatiramer acetate	1141:1160	Five (29%) of the MS participants had never been exposed to a DMD pre-stool sample and 12 (71%) had (7 to beta-interferon and 5 glatiramer acetate).					
35500534	2	95	theme	Canadian	345:352	arg1	study					395:399	the Canadian Paediatric Demyelinating Disease Network study	341:399	the Canadian Paediatric Demyelinating Disease Network study	341:399	METHODS We included 17 MS participants and 20 controls similar for sex, age, race, and stool consistency from the Canadian Paediatric Demyelinating Disease Network study.					
35500534	10	96	dep	abundance	1778:1786	arg1	p<0.049					1807:1813	p<0.049	1807:1813	p<0.049	1807:1813	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	10	97	theme	exposed	1727:1733	arg1	verses					1735:1740	the DMD exposed verses	1719:1740	the DMD exposed verses DMD naïve MS participants	1719:1766	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	3	98	used	used	452:455	arg2	abundances					436:445	Stool-derived gut metagenome gene abundances	402:445	Stool-derived gut metagenome gene abundances	402:445	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	3	99	theme	gene	431:434	arg1	abundances					436:445	Stool-derived gut metagenome gene abundances	402:445	Stool-derived gut metagenome gene abundances	402:445	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	7	100	theme	MS	1033:1034	arg1	participants					1036:1047	the MS participants	1029:1047	the MS participants	1029:1047	Five (29%) of the MS participants had never been exposed to a DMD pre-stool sample and 12 (71%) had (7 to beta-interferon and 5 glatiramer acetate).					
35500534	3	101	theme	relative	469:476	arg1	abundances					478:487	relative abundances	469:487	relative abundances	469:487	Stool-derived gut metagenome gene abundances were used to estimate relative abundances and turnover scores of individual microbial metabolites and the composition and diversity of carbohydrate-active enzymes (CAZymes).					
35500534	10	102	theme	MS	1752:1753	arg1	participants					1755:1766	naïve MS participants	1746:1766	naïve MS participants	1746:1766	Further, although CAZymes diversity did not differ (p>0.050), starch-degrading subfamilies were underrepresented in MS participants versus controls (relative abundance differences >-0.34, p<0.040) and in the DMD exposed verses DMD naïve MS participants (relative abundance differences>-0.20, p<0.049).					
35500534	1	103	theme	symptom	205:211	arg1	years					223:227	symptom onset <18 years	205:227	symptom onset <18 years	205:227	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	1	103	theme	symptom	205:211	arg1	patients					195:202	paediatric-onset MS patients	175:202	paediatric-onset MS patients (symptom onset <18 years)	175:228	BACKGROUND The aim of this study was to examine the gut microbiome's metabolic potential in paediatric-onset MS patients (symptom onset <18 years).					
35500534	5	104	theme	MS	935:936	arg1	participants/controls					938:958	the MS participants/controls	931:958	the MS participants/controls	931:958	RESULTS The median age(s) at MS symptom onset=16.1 years (interquartile range [IQR]=1.7), and at stool sample procurement=16.9/15.8 years (IQR=2.0/1.4), for the MS participants/controls.					
35500534	0	105	theme	metabolic	4:12	arg1	potential					14:22	The metabolic potential	0:22	The metabolic potential of the paediatric-onset multiple sclerosis gut	0:69	The metabolic potential of the paediatric-onset multiple sclerosis gut microbiome.					
35029088	8	0	theme	nanofiber	1118:1126	arg1	bundles					1128:1134	the CS/PEG nanofiber bundles	1107:1134	the CS/PEG nanofiber bundles	1107:1134	Transmission electron microscope (TEM) and Raman spectroscopy proved that the CNTs were well dispersed in the CS/PEG matrix and combined with the CS/PEG nanofiber bundles.					
35029088	12	1	theme	adhesion	1397:1404	arg1	rate					1406:1409	The electrical conductivity and cell adhesion rate	1360:1409	The electrical conductivity and cell adhesion rate of the scaffolds	1360:1426	The electrical conductivity and cell adhesion rate of the scaffolds increased with increasing MWCNT content.					
35029088	6	2	theme	physical	783:790	arg1	properties					805:814	Their physical and chemical properties	777:814	Their physical and chemical properties	777:814	Their physical and chemical properties and biocompatibility were evaluated.					
35029088	7	3	theme	Scanning	853:860	arg1	microscopy					871:880	Scanning electron microscopy	853:880	Scanning electron microscopy (SEM)	853:886	Scanning electron microscopy (SEM) showed that the composite scaffolds had a highly connected porous structure.					
35029088	7	3	theme	Scanning	853:860	arg1	SEM					883:885	SEM	883:885	SEM	883:885	Scanning electron microscopy (SEM) showed that the composite scaffolds had a highly connected porous structure.					
35029088	14	4	from	changes	1613:1619	arg1	expression					1628:1637	the expression	1624:1637	the expression of nerve cell markers	1624:1659	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	15	5	theme	good	1974:1977	arg1	biocompatibility					1979:1994	good biocompatibility	1974:1994	good biocompatibility	1974:1994	Preliminary research showed that the prepared CS/PEG/CNT scaffold has good biocompatibility and can be further applied to neural tissue engineering research.					
35029088	5	6	theme	CNT	700:702	arg1	content					704:710	CNT content	700:710	CNT content of 1%, 3%, and 5% (1%=0.01 g/mL)	700:743	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying.					
35029088	14	7	theme	polymerase	1687:1696	arg1	reaction					1704:1711	quantitative real-time polymerase chain reaction	1664:1711	quantitative real-time polymerase chain reaction (qRT-PCR)	1664:1721	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	14	7	theme	polymerase	1687:1696	arg1	qRT-PCR					1714:1720	qRT-PCR	1714:1720	qRT-PCR	1714:1720	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	12	8	theme	electrical	1364:1373	arg1	conductivity					1375:1386	electrical conductivity	1364:1386	electrical conductivity	1364:1386	The electrical conductivity and cell adhesion rate of the scaffolds increased with increasing MWCNT content.					
35029088	8	9	theme	Transmission	965:976	arg1	TEM					999:1001	TEM	999:1001	TEM	999:1001	Transmission electron microscope (TEM) and Raman spectroscopy proved that the CNTs were well dispersed in the CS/PEG matrix and combined with the CS/PEG nanofiber bundles.					
35029088	8	9	theme	Transmission	965:976	arg1	microscope					987:996	Transmission electron microscope	965:996	Transmission electron microscope (TEM)	965:1002	Transmission electron microscope (TEM) and Raman spectroscopy proved that the CNTs were well dispersed in the CS/PEG matrix and combined with the CS/PEG nanofiber bundles.					
35029088	1	10	theme	neural	196:201	arg1	engineering					210:220	neural tissue engineering	196:220	neural tissue engineering	196:220	Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings.					
35029088	5	11	theme	CS/PEG/CNT	664:673	arg1	scaffolds					685:693	CS/PEG/CNT composite scaffolds	664:693	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL)	664:743	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying.					
35029088	11	12	theme	swelling	1272:1279	arg1	state					1281:1285	a stable water swelling state	1257:1285	a stable water swelling state	1257:1285	They reached a stable water swelling state within 24 h, and swelling decreased with increasing MWCNT concentration.					
35029088	14	13	theme	real-time	1677:1685	arg1	reaction					1704:1711	quantitative real-time polymerase chain reaction	1664:1711	quantitative real-time polymerase chain reaction (qRT-PCR)	1664:1721	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	14	13	theme	real-time	1677:1685	arg1	qRT-PCR					1714:1720	qRT-PCR	1714:1720	qRT-PCR	1714:1720	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	14	14	theme	factor	1837:1842	arg1	NGFR					1854:1857	NGFR	1854:1857	NGFR	1854:1857	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	14	14	theme	factor	1837:1842	arg1	receptor					1844:1851	nerve growth factor receptor	1824:1851	nerve growth factor receptor (NGFR)	1824:1858	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	2	15	dep	excellent	264:272	arg1	mimics					274:279	mimics	274:279	mimics of extracellular matrix due to their hydrophilicity, viscosity, and biocompatibility	274:364	CNT scaffolds are excellent mimics of extracellular matrix due to their hydrophilicity, viscosity, and biocompatibility.					
35029088	13	16	theme	PC12	1522:1525	arg1	cells					1528:1532	rat pheochromocytoma (PC12) cells	1500:1532	rat pheochromocytoma (PC12) cells grown in the scaffolds	1500:1555	Immunofluorescence showed that rat pheochromocytoma (PC12) cells grown in the scaffolds had characteristics similar to nerve cells.					
35029088	14	17	theme	nerve	1824:1828	arg1	NGFR					1854:1857	NGFR	1854:1857	NGFR	1854:1857	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	14	17	theme	nerve	1824:1828	arg1	receptor					1844:1851	nerve growth factor receptor	1824:1851	nerve growth factor receptor (NGFR)	1824:1858	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	14	18	theme	cell	1648:1651	arg1	markers					1653:1659	nerve cell markers	1642:1659	nerve cell markers	1642:1659	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	2	19	theme	CNT	246:248	arg1	scaffolds					250:258	CNT scaffolds	246:258	CNT scaffolds	246:258	CNT scaffolds are excellent mimics of extracellular matrix due to their hydrophilicity, viscosity, and biocompatibility.					
35029088	1	20	theme	composite	145:153	arg1	materials					155:163	Carbon nanotube (CNT) composite materials	123:163	Carbon nanotube (CNT) composite materials	123:163	Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings.					
35029088	1	20	theme	composite	145:153	arg1	coatings					236:243	coatings	236:243	coatings	236:243	Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings.					
35029088	5	21	theme	%	716:716	arg1	content					704:710	CNT content	700:710	CNT content of 1%, 3%, and 5% (1%=0.01 g/mL)	700:743	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying.					
35029088	2	22	theme	extracellular	284:296	arg1	matrix					298:303	extracellular matrix	284:303	extracellular matrix	284:303	CNT scaffolds are excellent mimics of extracellular matrix due to their hydrophilicity, viscosity, and biocompatibility.					
35029088	5	23	theme	%	732:732	arg1	%					716:716	1%	715:716	1%	715:716	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying.					
35029088	5	23	theme	%	732:732	arg1	g/mL					739:742	1%=0.01 g/mL	731:742	1%=0.01 g/mL	731:742	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying.					
35029088	13	24	theme	pheochromocytoma	1504:1519	arg1	cells					1528:1532	rat pheochromocytoma (PC12) cells	1500:1532	rat pheochromocytoma (PC12) cells grown in the scaffolds	1500:1555	Immunofluorescence showed that rat pheochromocytoma (PC12) cells grown in the scaffolds had characteristics similar to nerve cells.					
35029088	10	25	theme	83	1232:1233	arg1	%					1234:1234	%	1234:1234	%	1234:1234	The porosity of the scaffolds ranged from 83% to 96%.					
35029088	7	26	theme	connected	937:945	arg1	structure					954:962	a highly connected porous structure	928:962	a highly connected porous structure	928:962	Scanning electron microscopy (SEM) showed that the composite scaffolds had a highly connected porous structure.					
35029088	10	27	dep	%	1234:1234	arg1	to					1236:1237	to	1236:1237	to	1236:1237	The porosity of the scaffolds ranged from 83% to 96%.					
35029088	3	28	theme	cell	477:480	arg1	behavior					482:489	neuronal cell behavior	468:489	neuronal cell behavior	468:489	CNTs can also impart conductivity to other insulating materials, improve mechanical stability, guide neuronal cell behavior, and trigger axon regeneration.					
35029088	0	29	theme	Biocompatible	0:12	arg1	composite					72:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite	0:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite	0:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering.					
35029088	1	30	theme	nanotube	130:137	arg1	materials					155:163	Carbon nanotube (CNT) composite materials	123:163	Carbon nanotube (CNT) composite materials	123:163	Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings.					
35029088	1	30	theme	nanotube	130:137	arg1	coatings					236:243	coatings	236:243	coatings	236:243	Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings.					
35029088	3	31	theme	other	404:408	arg1	materials					421:429	other insulating materials	404:429	other insulating materials	404:429	CNTs can also impart conductivity to other insulating materials, improve mechanical stability, guide neuronal cell behavior, and trigger axon regeneration.					
35029088	15	32	theme	prepared	1941:1948	arg1	scaffold					1961:1968	the prepared CS/PEG/CNT scaffold	1937:1968	the prepared CS/PEG/CNT scaffold	1937:1968	Preliminary research showed that the prepared CS/PEG/CNT scaffold has good biocompatibility and can be further applied to neural tissue engineering research.					
35029088	13	33	contain	had	1557:1559	arg2	characteristics					1561:1575	characteristics	1561:1575	characteristics similar to nerve cells	1561:1598	Immunofluorescence showed that rat pheochromocytoma (PC12) cells grown in the scaffolds had characteristics similar to nerve cells.					
35029088	13	33	contain	had	1557:1559	arg1	cells					1528:1532	rat pheochromocytoma (PC12) cells	1500:1532	rat pheochromocytoma (PC12) cells grown in the scaffolds	1500:1555	Immunofluorescence showed that rat pheochromocytoma (PC12) cells grown in the scaffolds had characteristics similar to nerve cells.					
35029088	15	34	theme	engineering	2040:2050	arg1	research					2052:2059	neural tissue engineering research	2026:2059	neural tissue engineering research	2026:2059	Preliminary research showed that the prepared CS/PEG/CNT scaffold has good biocompatibility and can be further applied to neural tissue engineering research.					
35029088	4	35	theme	composite	582:590	arg1	scaffolds					592:600	chitosan (CS)/polyethylene glycol (PEG) composite scaffolds	542:600	chitosan (CS)/polyethylene glycol (PEG) composite scaffolds	542:600	The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs).					
35029088	0	36	theme	nanotube	63:70	arg1	composite					72:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite	0:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite	0:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering.					
35029088	13	37	theme	nerve	1588:1592	arg1	cells					1594:1598	nerve cells	1588:1598	nerve cells	1588:1598	Immunofluorescence showed that rat pheochromocytoma (PC12) cells grown in the scaffolds had characteristics similar to nerve cells.					
35029088	0	38	theme	neural	96:101	arg1	engineering					110:120	neural tissue engineering	96:120	neural tissue engineering	96:120	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering.					
35029088	4	39	dep	chitosan	542:549	arg1	CS					552:553	CS	552:553	CS	552:553	The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs).					
35029088	8	40	theme	CS/PEG	1075:1080	arg1	matrix					1082:1087	the CS/PEG matrix	1071:1087	the CS/PEG matrix	1071:1087	Transmission electron microscope (TEM) and Raman spectroscopy proved that the CNTs were well dispersed in the CS/PEG matrix and combined with the CS/PEG nanofiber bundles.					
35029088	4	41	theme	glycol	569:574	arg1	scaffolds					592:600	chitosan (CS)/polyethylene glycol (PEG) composite scaffolds	542:600	chitosan (CS)/polyethylene glycol (PEG) composite scaffolds	542:600	The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs).					
35029088	6	42	theme	chemical	796:803	arg1	properties					805:814	Their physical and chemical properties	777:814	Their physical and chemical properties	777:814	Their physical and chemical properties and biocompatibility were evaluated.					
35029088	15	43	theme	tissue	2033:2038	arg1	research					2052:2059	neural tissue engineering research	2026:2059	neural tissue engineering research	2026:2059	Preliminary research showed that the prepared CS/PEG/CNT scaffold has good biocompatibility and can be further applied to neural tissue engineering research.					
35029088	5	44	theme	composite	675:683	arg1	scaffolds					685:693	CS/PEG/CNT composite scaffolds	664:693	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL)	664:743	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying.					
35029088	8	45	theme	CS/PEG	1111:1116	arg1	bundles					1128:1134	the CS/PEG nanofiber bundles	1107:1134	the CS/PEG nanofiber bundles	1107:1134	Transmission electron microscope (TEM) and Raman spectroscopy proved that the CNTs were well dispersed in the CS/PEG matrix and combined with the CS/PEG nanofiber bundles.					
35029088	5	46	with	scaffolds	685:693	arg1	content					704:710	CNT content	700:710	CNT content of 1%, 3%, and 5% (1%=0.01 g/mL)	700:743	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying.					
35029088	9	47	theme	elastic	1157:1163	arg1	modulus					1165:1171	the elastic modulus	1153:1171	the elastic modulus of the scaffold	1153:1187	MWCNTs enhanced the elastic modulus of the scaffold.					
35029088	7	48	theme	electron	862:869	arg1	microscopy					871:880	Scanning electron microscopy	853:880	Scanning electron microscopy (SEM)	853:886	Scanning electron microscopy (SEM) showed that the composite scaffolds had a highly connected porous structure.					
35029088	7	48	theme	electron	862:869	arg1	SEM					883:885	SEM	883:885	SEM	883:885	Scanning electron microscopy (SEM) showed that the composite scaffolds had a highly connected porous structure.					
35029088	14	49	theme	chain	1698:1702	arg1	reaction					1704:1711	quantitative real-time polymerase chain reaction	1664:1711	quantitative real-time polymerase chain reaction (qRT-PCR)	1664:1721	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	14	49	theme	chain	1698:1702	arg1	qRT-PCR					1714:1720	qRT-PCR	1714:1720	qRT-PCR	1714:1720	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	12	50	theme	conductivity	1375:1386	arg1	rate					1406:1409	The electrical conductivity and cell adhesion rate	1360:1409	The electrical conductivity and cell adhesion rate of the scaffolds	1360:1426	The electrical conductivity and cell adhesion rate of the scaffolds increased with increasing MWCNT content.					
35029088	8	51	theme	electron	978:985	arg1	TEM					999:1001	TEM	999:1001	TEM	999:1001	Transmission electron microscope (TEM) and Raman spectroscopy proved that the CNTs were well dispersed in the CS/PEG matrix and combined with the CS/PEG nanofiber bundles.					
35029088	8	51	theme	electron	978:985	arg1	microscope					987:996	Transmission electron microscope	965:996	Transmission electron microscope (TEM)	965:1002	Transmission electron microscope (TEM) and Raman spectroscopy proved that the CNTs were well dispersed in the CS/PEG matrix and combined with the CS/PEG nanofiber bundles.					
35029088	11	52	theme	MWCNT	1339:1343	arg1	concentration					1345:1357	MWCNT concentration	1339:1357	MWCNT concentration	1339:1357	They reached a stable water swelling state within 24 h, and swelling decreased with increasing MWCNT concentration.					
35029088	13	53	theme	similar	1577:1583	arg1	characteristics					1561:1575	characteristics	1561:1575	characteristics similar to nerve cells	1561:1598	Immunofluorescence showed that rat pheochromocytoma (PC12) cells grown in the scaffolds had characteristics similar to nerve cells.					
35029088	12	54	theme	cell	1392:1395	arg1	adhesion					1397:1404	cell adhesion	1392:1404	cell adhesion	1392:1404	The electrical conductivity and cell adhesion rate of the scaffolds increased with increasing MWCNT content.					
35029088	9	55	theme	scaffold	1180:1187	arg1	modulus					1165:1171	the elastic modulus	1153:1171	the elastic modulus of the scaffold	1153:1187	MWCNTs enhanced the elastic modulus of the scaffold.					
35029088	1	56	theme	tissue	203:208	arg1	engineering					210:220	neural tissue engineering	196:220	neural tissue engineering	196:220	Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings.					
35029088	15	57	contain	has	1970:1972	arg1	scaffold					1961:1968	the prepared CS/PEG/CNT scaffold	1937:1968	the prepared CS/PEG/CNT scaffold	1937:1968	Preliminary research showed that the prepared CS/PEG/CNT scaffold has good biocompatibility and can be further applied to neural tissue engineering research.					
35029088	15	57	contain	has	1970:1972	arg2	biocompatibility					1979:1994	good biocompatibility	1974:1994	good biocompatibility	1974:1994	Preliminary research showed that the prepared CS/PEG/CNT scaffold has good biocompatibility and can be further applied to neural tissue engineering research.					
35029088	11	58	theme	water	1266:1270	arg1	state					1281:1285	a stable water swelling state	1257:1285	a stable water swelling state	1257:1285	They reached a stable water swelling state within 24 h, and swelling decreased with increasing MWCNT concentration.					
35029088	5	59	theme	%	720:720	arg1	content					704:710	CNT content	700:710	CNT content of 1%, 3%, and 5% (1%=0.01 g/mL)	700:743	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying.					
35029088	14	60	theme	quantitative	1664:1675	arg1	reaction					1704:1711	quantitative real-time polymerase chain reaction	1664:1711	quantitative real-time polymerase chain reaction (qRT-PCR)	1664:1721	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	14	60	theme	quantitative	1664:1675	arg1	qRT-PCR					1714:1720	qRT-PCR	1714:1720	qRT-PCR	1714:1720	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	14	61	theme	growth	1830:1835	arg1	NGFR					1854:1857	NGFR	1854:1857	NGFR	1854:1857	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	14	61	theme	growth	1830:1835	arg1	receptor					1844:1851	nerve growth factor receptor	1824:1851	nerve growth factor receptor (NGFR)	1824:1858	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	7	62	contain	had	924:926	arg1	scaffolds					914:922	the composite scaffolds	900:922	the composite scaffolds	900:922	Scanning electron microscopy (SEM) showed that the composite scaffolds had a highly connected porous structure.					
35029088	7	62	contain	had	924:926	arg2	structure					954:962	a highly connected porous structure	928:962	a highly connected porous structure	928:962	Scanning electron microscopy (SEM) showed that the composite scaffolds had a highly connected porous structure.					
35029088	14	63	theme	markers	1653:1659	arg1	expression					1628:1637	the expression	1624:1637	the expression of nerve cell markers	1624:1659	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	10	64	theme	scaffolds	1210:1218	arg1	porosity					1194:1201	The porosity	1190:1201	The porosity of the scaffolds	1190:1218	The porosity of the scaffolds ranged from 83% to 96%.					
35029088	14	65	theme	nerve	1642:1646	arg1	markers					1653:1659	nerve cell markers	1642:1659	nerve cell markers	1642:1659	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	7	66	theme	porous	947:952	arg1	structure					954:962	a highly connected porous structure	928:962	a highly connected porous structure	928:962	Scanning electron microscopy (SEM) showed that the composite scaffolds had a highly connected porous structure.					
35029088	0	67	theme	tissue	103:108	arg1	engineering					110:120	neural tissue engineering	96:120	neural tissue engineering	96:120	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering.					
35029088	13	68	theme	rat	1500:1502	arg1	cells					1528:1532	rat pheochromocytoma (PC12) cells	1500:1532	rat pheochromocytoma (PC12) cells grown in the scaffolds	1500:1555	Immunofluorescence showed that rat pheochromocytoma (PC12) cells grown in the scaffolds had characteristics similar to nerve cells.					
35029088	4	69	theme	chitosan	542:549	arg1	glycol					569:574	chitosan (CS)/polyethylene glycol	542:574	chitosan (CS)/polyethylene glycol (PEG) composite scaffolds	542:600	The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs).					
35029088	4	69	theme	chitosan	542:549	arg1	PEG					577:579	PEG	577:579	PEG	577:579	The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs).					
35029088	5	70	theme	=0.01	733:737	arg1	%					716:716	1%	715:716	1%	715:716	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying.					
35029088	5	70	theme	=0.01	733:737	arg1	g/mL					739:742	1%=0.01 g/mL	731:742	1%=0.01 g/mL	731:742	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying.					
35029088	8	71	theme	Raman	1008:1012	arg1	spectroscopy					1014:1025	Raman spectroscopy	1008:1025	Raman spectroscopy	1008:1025	Transmission electron microscope (TEM) and Raman spectroscopy proved that the CNTs were well dispersed in the CS/PEG matrix and combined with the CS/PEG nanofiber bundles.					
35029088	14	72	theme	growth-associated	1786:1802	arg1	protein					1804:1810	growth-associated protein 43	1786:1813	growth-associated protein 43 (GAP43)	1786:1821	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	14	72	theme	growth-associated	1786:1802	arg1	GAP43					1816:1820	GAP43	1816:1820	GAP43	1816:1820	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	0	73	theme	glycol/multi-walled	36:54	arg1	composite					72:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite	0:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite	0:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering.					
35029088	1	74	from	use	189:191	arg1	engineering					210:220	neural tissue engineering	196:220	neural tissue engineering	196:220	Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings.					
35029088	5	75	theme	%	728:728	arg1	content					704:710	CNT content	700:710	CNT content of 1%, 3%, and 5% (1%=0.01 g/mL)	700:743	CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying.					
35029088	11	76	theme	stable	1259:1264	arg1	state					1281:1285	a stable water swelling state	1257:1285	a stable water swelling state	1257:1285	They reached a stable water swelling state within 24 h, and swelling decreased with increasing MWCNT concentration.					
35029088	10	77	theme	96	1239:1240	arg1	%					1234:1234	%	1234:1234	%	1234:1234	The porosity of the scaffolds ranged from 83% to 96%.					
35029088	0	78	theme	chitosan/polyethylene	14:34	arg1	composite					72:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite	0:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite	0:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering.					
35029088	1	79	theme	Carbon	123:128	arg1	nanotube					130:137	Carbon nanotube	123:137	Carbon nanotube (CNT) composite materials	123:163	Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings.					
35029088	1	79	theme	Carbon	123:128	arg1	CNT					140:142	CNT	140:142	CNT	140:142	Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings.					
35029088	3	80	theme	neuronal	468:475	arg1	behavior					482:489	neuronal cell behavior	468:489	neuronal cell behavior	468:489	CNTs can also impart conductivity to other insulating materials, improve mechanical stability, guide neuronal cell behavior, and trigger axon regeneration.					
35029088	0	81	theme	carbon	56:61	arg1	composite					72:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite	0:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite	0:80	Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering.					
35029088	7	82	theme	composite	904:912	arg1	scaffolds					914:922	the composite scaffolds	900:922	the composite scaffolds	900:922	Scanning electron microscopy (SEM) showed that the composite scaffolds had a highly connected porous structure.					
35029088	3	83	theme	insulating	410:419	arg1	materials					421:429	other insulating materials	404:429	other insulating materials	404:429	CNTs can also impart conductivity to other insulating materials, improve mechanical stability, guide neuronal cell behavior, and trigger axon regeneration.					
35029088	4	84	theme	scaffolds	592:600	arg1	performance					527:537	The performance	523:537	The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds	523:600	The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs).					
35029088	15	85	theme	CS/PEG/CNT	1950:1959	arg1	scaffold					1961:1968	the prepared CS/PEG/CNT scaffold	1937:1968	the prepared CS/PEG/CNT scaffold	1937:1968	Preliminary research showed that the prepared CS/PEG/CNT scaffold has good biocompatibility and can be further applied to neural tissue engineering research.					
35029088	1	86	dep	attractive	174:183	arg1	materials					155:163	Carbon nanotube (CNT) composite materials	123:163	Carbon nanotube (CNT) composite materials	123:163	Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings.					
35029088	1	86	dep	attractive	174:183	arg1	coatings					236:243	coatings	236:243	coatings	236:243	Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings.					
35029088	3	87	theme	axon	504:507	arg1	regeneration					509:520	axon regeneration	504:520	axon regeneration	504:520	CNTs can also impart conductivity to other insulating materials, improve mechanical stability, guide neuronal cell behavior, and trigger axon regeneration.					
35029088	12	88	theme	MWCNT	1454:1458	arg1	content					1460:1466	MWCNT content	1454:1466	MWCNT content	1454:1466	The electrical conductivity and cell adhesion rate of the scaffolds increased with increasing MWCNT content.					
35029088	4	89	theme	/polyethylene	555:567	arg1	glycol					569:574	chitosan (CS)/polyethylene glycol	542:574	chitosan (CS)/polyethylene glycol (PEG) composite scaffolds	542:600	The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs).					
35029088	4	89	theme	/polyethylene	555:567	arg1	PEG					577:579	PEG	577:579	PEG	577:579	The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs).					
35029088	14	90	theme	PC12	1739:1742	arg1	cells					1744:1748	PC12 cells	1739:1748	PC12 cells cultured in the scaffolds	1739:1774	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	14	91	theme	TUBB3	1887:1891	arg1	proteins					1894:1901	class III β‍-tubulin (TUBB3) proteins	1865:1901	class III β‍-tubulin (TUBB3) proteins	1865:1901	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	3	92	theme	mechanical	440:449	arg1	stability					451:459	mechanical stability	440:459	mechanical stability	440:459	CNTs can also impart conductivity to other insulating materials, improve mechanical stability, guide neuronal cell behavior, and trigger axon regeneration.					
35029088	12	93	theme	scaffolds	1418:1426	arg1	rate					1406:1409	The electrical conductivity and cell adhesion rate	1360:1409	The electrical conductivity and cell adhesion rate of the scaffolds	1360:1426	The electrical conductivity and cell adhesion rate of the scaffolds increased with increasing MWCNT content.					
35029088	14	94	theme	β‍-tubulin	1875:1884	arg1	proteins					1894:1901	class III β‍-tubulin (TUBB3) proteins	1865:1901	class III β‍-tubulin (TUBB3) proteins	1865:1901	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	4	95	theme	multi-walled	636:647	arg1	MWCNTs					655:660	MWCNTs	655:660	MWCNTs	655:660	The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs).					
35029088	4	95	theme	multi-walled	636:647	arg1	CNTs					649:652	multi-walled CNTs	636:652	multi-walled CNTs (MWCNTs)	636:661	The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs).					
35029088	15	96	theme	Preliminary	1904:1914	arg1	research					1916:1923	Preliminary research	1904:1923	Preliminary research	1904:1923	Preliminary research showed that the prepared CS/PEG/CNT scaffold has good biocompatibility and can be further applied to neural tissue engineering research.					
35029088	14	97	theme	class	1865:1869	arg1	proteins					1894:1901	class III β‍-tubulin (TUBB3) proteins	1865:1901	class III β‍-tubulin (TUBB3) proteins	1865:1901	We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins.					
35029088	15	98	theme	neural	2026:2031	arg1	research					2052:2059	neural tissue engineering research	2026:2059	neural tissue engineering research	2026:2059	Preliminary research showed that the prepared CS/PEG/CNT scaffold has good biocompatibility and can be further applied to neural tissue engineering research.					
37146305	4	0	theme	selective	921:929	arg1	separation					931:940	the selective separation	917:940	the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction	917:1146	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	4	1	theme	intact	1094:1099	arg1	glycopeptides					1101:1113	85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides	1007:1113	85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction	1007:1146	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	3	2	theme	simultaneous	717:728	arg1	enrichment					730:739	simultaneous enrichment	717:739	simultaneous enrichment	717:739	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	6	3	theme	types	1556:1560	arg1	co-regulation					1529:1541	potential co-regulation	1519:1541	potential co-regulation of different types of glycosylation	1519:1577	Interestingly, five glycoproteins containing both significant regulation of N- and O-glycosylation were observed, hinting potential co-regulation of different types of glycosylation during tumor progress.					
37146305	4	4	link	O-linked	952:959	arg1	glycopeptides					968:980	O-linked intact glycopeptides	952:980	O-linked intact glycopeptides	952:980	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	1	5	theme	N-/O-linked	279:289	arg1	glycans					305:311	N-/O-linked site-specific glycans	279:311	N-/O-linked site-specific glycans	279:311	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	5	6	gly	glycopeptides	1382:1394	arg2	glycopeptides					1382:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	1	7	theme	essential	192:200	arg1	roles					202:206	essential roles	192:206	essential roles	192:206	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	1	8	theme	site-specific	291:303	arg1	glycans					305:311	N-/O-linked site-specific glycans	279:311	N-/O-linked site-specific glycans	279:311	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	3	9	link	O-linked	772:779	arg1	glycopeptides					788:800	O-linked intact glycopeptides	772:800	O-linked intact glycopeptides	772:800	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	7	10	theme	useful	1742:1747	arg1	platform					1630:1637	this integrated platform	1614:1637	this integrated platform	1614:1637	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	7	10	theme	useful	1742:1747	arg1	tool					1749:1752	a useful tool	1740:1752	a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale	1740:1837	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	3	11	theme	intact	781:786	arg1	glycopeptides					788:800	O-linked intact glycopeptides	772:800	O-linked intact glycopeptides	772:800	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	0	12	theme	Glycopeptides	117:129	arg1	Characterization					74:89	Characterization	74:89	Characterization	74:89	Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides.					
37146305	0	12	theme	Glycopeptides	117:129	arg1	Enrichment					59:68	Simultaneous Enrichment	46:68	Simultaneous Enrichment	46:68	Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides.					
37146305	2	13	theme	glycopeptides	570:582	arg1	enrichment					540:549	the enrichment	536:549	the enrichment of O-linked intact glycopeptides	536:582	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	2	14	theme	glycosylation	464:476	arg1	procedures					521:530	the time-consuming and tedious procedures	490:530	the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides	490:582	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	2	14	theme	glycosylation	464:476	arg1	nature					442:447	the micro-heterogeneity and low abundance nature	400:447	the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation	400:476	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	3	15	theme	same	811:814	arg1	sample					822:827	the same serum sample	807:827	the same serum sample	807:827	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	7	16	theme	glycosylation	1709:1721	arg1	analysis					1689:1696	the global analysis	1678:1696	the global analysis of protein glycosylation	1678:1721	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	4	17	theme	intact	961:966	arg1	glycopeptides					968:980	O-linked intact glycopeptides	952:980	O-linked intact glycopeptides	952:980	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	5	18	link	O-linked	1353:1360	arg1	glycopeptides					1382:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	6	19	theme	potential	1519:1527	arg1	co-regulation					1529:1541	potential co-regulation	1519:1541	potential co-regulation of different types of glycosylation	1519:1577	Interestingly, five glycoproteins containing both significant regulation of N- and O-glycosylation were observed, hinting potential co-regulation of different types of glycosylation during tumor progress.					
37146305	1	20	theme	N-linked	137:144	arg1	glycosylation					146:158	N-linked glycosylation	137:158	N-linked glycosylation	137:158	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	4	21	theme	intact	1022:1027	arg1	glycopeptides					1029:1041	O-linked intact glycopeptides	1013:1041	85.1% O-linked intact glycopeptides presented in the first fraction	1007:1073	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	5	22	theme	O-linked	1353:1360	arg1	glycopeptides					1382:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	6	23	theme	N-	1473:1474	arg1	regulation					1459:1468	both significant regulation	1442:1468	both significant regulation of N- and O-glycosylation	1442:1494	Interestingly, five glycoproteins containing both significant regulation of N- and O-glycosylation were observed, hinting potential co-regulation of different types of glycosylation during tumor progress.					
37146305	7	24	theme	proteomics	1822:1831	arg1	scale					1833:1837	the proteomics scale	1818:1837	the proteomics scale	1818:1837	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	7	25	theme	global	1682:1687	arg1	analysis					1689:1696	the global analysis	1678:1696	the global analysis of protein glycosylation	1678:1721	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	2	26	theme	tedious	513:519	arg1	procedures					521:530	the time-consuming and tedious procedures	490:530	the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides	490:582	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	4	27	link	N-linked	1085:1092	arg1	glycopeptides					1101:1113	85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides	1007:1113	85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction	1007:1146	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	2	28	theme	great	590:594	arg1	challenges					596:605	great challenges	590:605	great challenges for their efficient and accurate characterization	590:655	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	7	29	theme	N-/O-linked	1782:1792	arg1	glycopeptides					1801:1813	N-/O-linked intact glycopeptides	1782:1813	N-/O-linked intact glycopeptides	1782:1813	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	2	30	gly	glycopeptides	570:582	arg2	glycopeptides					570:582	O-linked intact glycopeptides	554:582	O-linked intact glycopeptides	554:582	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	2	31	theme	O-linked	554:561	arg1	glycopeptides					570:582	O-linked intact glycopeptides	554:582	O-linked intact glycopeptides	554:582	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	5	32	link	N-linked	1366:1373	arg1	glycopeptides					1382:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	3	33	from	sample	822:827	arg1	characterization					745:760	characterization	745:760	characterization	745:760	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	3	33	from	sample	822:827	arg1	enrichment					730:739	simultaneous enrichment	717:739	simultaneous enrichment	717:739	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	2	34	link	N-/O-linked	452:462	arg1	glycosylation					464:476	N-/O-linked glycosylation	452:476	N-/O-linked glycosylation	452:476	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	0	35	theme	N-	94:95	arg1	Characterization					74:89	Characterization	74:89	Characterization	74:89	Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides.					
37146305	0	35	theme	N-	94:95	arg1	Enrichment					59:68	Simultaneous Enrichment	46:68	Simultaneous Enrichment	46:68	Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides.					
37146305	4	36	with	fractions	991:999	arg1	glycopeptides					1101:1113	85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides	1007:1113	85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction	1007:1146	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	7	37	theme	useful	1660:1665	arg1	avenue					1667:1672	a potentially useful avenue	1646:1672	a potentially useful avenue for the global analysis of protein glycosylation	1646:1721	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	5	38	theme	cancer	1278:1283	arg1	samples					1259:1265	serum samples	1253:1265	serum samples	1253:1265	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	5	39	theme	high	1165:1168	arg1	reproducibility					1170:1184	high reproducibility	1165:1184	high reproducibility	1165:1184	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	1	40	dep	onset	215:219	arg1	the					211:213	the	211:213	the	211:213	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	5	41	theme	differential	1228:1239	arg1	analysis					1241:1248	the differential analysis	1224:1248	the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides	1224:1394	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	7	42	theme	intact	1794:1799	arg1	glycopeptides					1801:1813	N-/O-linked intact glycopeptides	1782:1813	N-/O-linked intact glycopeptides	1782:1813	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	4	43	theme	N-linked	1085:1092	arg1	glycopeptides					1101:1113	85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides	1007:1113	85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction	1007:1146	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	1	44	link	O-linked	164:171	arg1	glycosylation					173:185	O-linked glycosylation	164:185	O-linked glycosylation	164:185	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	5	45	theme	N-linked	1366:1373	arg1	glycopeptides					1382:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	0	46	dep	Enrichment	59:68	arg1	the					42:44	the	42:44	the	42:44	Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides.					
37146305	2	47	theme	time-consuming	494:507	arg1	procedures					521:530	the time-consuming and tedious procedures	490:530	the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides	490:582	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	2	48	theme	abundance	432:440	arg1	nature					442:447	the micro-heterogeneity and low abundance nature	400:447	the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation	400:476	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	0	49	theme	Platform	29:36	arg1	Development					0:10	Development	0:10	Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides.	0:130	Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides.					
37146305	7	50	theme	integrated	1619:1628	arg1	platform					1630:1637	this integrated platform	1614:1637	this integrated platform	1614:1637	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	7	50	theme	integrated	1619:1628	arg1	tool					1749:1752	a useful tool	1740:1752	a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale	1740:1837	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	6	51	theme	O-glycosylation	1480:1494	arg1	regulation					1459:1468	both significant regulation	1442:1468	both significant regulation of N- and O-glycosylation	1442:1494	Interestingly, five glycoproteins containing both significant regulation of N- and O-glycosylation were observed, hinting potential co-regulation of different types of glycosylation during tumor progress.					
37146305	4	52	theme	second	1132:1137	arg1	fraction					1139:1146	the second fraction	1128:1146	the second fraction	1128:1146	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	2	53	theme	low	428:430	arg1	abundance					432:440	low abundance	428:440	low abundance	428:440	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	4	54	theme	experimental	849:860	arg1	conditions					862:871	the experimental conditions	845:871	the experimental conditions	845:871	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	1	55	theme	diseases	248:255	arg1	onset					215:219	onset	215:219	onset	215:219	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	1	55	theme	diseases	248:255	arg1	progression					225:235	progression	225:235	progression	225:235	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	5	56	theme	samples	1259:1265	arg1	analysis					1241:1248	the differential analysis	1224:1248	the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides	1224:1394	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	2	57	theme	accurate	631:638	arg1	characterization					640:655	their efficient and accurate characterization	611:655	their efficient and accurate characterization	611:655	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	6	58	contain	containing	1431:1440	arg1	glycoproteins					1417:1429	five glycoproteins	1412:1429	five glycoproteins containing both significant regulation of N- and O-glycosylation	1412:1494	Interestingly, five glycoproteins containing both significant regulation of N- and O-glycosylation were observed, hinting potential co-regulation of different types of glycosylation during tumor progress.					
37146305	6	58	contain	containing	1431:1440	arg2	regulation					1459:1468	both significant regulation	1442:1468	both significant regulation of N- and O-glycosylation	1442:1494	Interestingly, five glycoproteins containing both significant regulation of N- and O-glycosylation were observed, hinting potential co-regulation of different types of glycosylation during tumor progress.					
37146305	1	59	link	N-/O-linked	279:289	arg1	glycans					305:311	N-/O-linked site-specific glycans	279:311	N-/O-linked site-specific glycans	279:311	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	6	60	theme	different	1546:1554	arg1	types					1556:1560	different types	1546:1560	different types of glycosylation	1546:1577	Interestingly, five glycoproteins containing both significant regulation of N- and O-glycosylation were observed, hinting potential co-regulation of different types of glycosylation during tumor progress.					
37146305	5	61	theme	gastric	1270:1276	arg1	cancer					1278:1283	gastric cancer	1270:1283	gastric cancer	1270:1283	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	7	62	gly	glycopeptides	1801:1813	arg2	glycopeptides					1801:1813	N-/O-linked intact glycopeptides	1782:1813	N-/O-linked intact glycopeptides	1782:1813	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	2	63	theme	efficient	617:625	arg1	characterization					640:655	their efficient and accurate characterization	611:655	their efficient and accurate characterization	611:655	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	3	64	theme	O-linked	772:779	arg1	glycopeptides					788:800	O-linked intact glycopeptides	772:800	O-linked intact glycopeptides	772:800	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	5	65	dep	17	1320:1321	arg1	glycopeptides					1382:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	7	66	from	scale	1833:1837	arg1	characterization					1762:1777	the characterization	1758:1777	the characterization of N-/O-linked intact glycopeptides at the proteomics scale	1758:1837	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	3	67	theme	N-	765:766	arg1	characterization					745:760	characterization	745:760	characterization	745:760	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	3	67	theme	N-	765:766	arg1	enrichment					730:739	simultaneous enrichment	717:739	simultaneous enrichment	717:739	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	4	68	dep	%	1011:1011	arg1	glycopeptides					1029:1041	O-linked intact glycopeptides	1013:1041	85.1% O-linked intact glycopeptides presented in the first fraction	1007:1073	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	4	69	theme	glycopeptides	968:980	arg1	separation					931:940	the selective separation	917:940	the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction	917:1146	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	3	70	theme	glycopeptides	788:800	arg1	characterization					745:760	characterization	745:760	characterization	745:760	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	3	70	theme	glycopeptides	788:800	arg1	enrichment					730:739	simultaneous enrichment	717:739	simultaneous enrichment	717:739	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	2	71	theme	intact	563:568	arg1	glycopeptides					570:582	O-linked intact glycopeptides	554:582	O-linked intact glycopeptides	554:582	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	4	72	gly	glycopeptides	968:980	arg2	glycopeptides					968:980	O-linked intact glycopeptides	952:980	O-linked intact glycopeptides	952:980	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	7	73	theme	protein	1701:1707	arg1	glycosylation					1709:1721	protein glycosylation	1701:1721	protein glycosylation	1701:1721	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	4	74	theme	N-	945:946	arg1	separation					931:940	the selective separation	917:940	the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction	917:1146	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	3	75	theme	serum	816:820	arg1	sample					822:827	the same serum sample	807:827	the same serum sample	807:827	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	4	76	gly	glycopeptides	1101:1113	arg2	glycopeptides					1101:1113	85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides	1007:1113	85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction	1007:1146	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	4	77	theme	O-linked	952:959	arg1	glycopeptides					968:980	O-linked intact glycopeptides	952:980	O-linked intact glycopeptides	952:980	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	5	78	theme	changed	1345:1351	arg1	glycopeptides					1382:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	4	79	theme	O-linked	1013:1020	arg1	glycopeptides					1029:1041	O-linked intact glycopeptides	1013:1041	85.1% O-linked intact glycopeptides presented in the first fraction	1007:1073	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	2	80	link	O-linked	554:561	arg1	glycopeptides					570:582	O-linked intact glycopeptides	554:582	O-linked intact glycopeptides	554:582	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	1	81	theme	O-linked	164:171	arg1	glycosylation					173:185	O-linked glycosylation	164:185	O-linked glycosylation	164:185	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	6	82	theme	significant	1447:1457	arg1	regulation					1459:1468	both significant regulation	1442:1468	both significant regulation of N- and O-glycosylation	1442:1494	Interestingly, five glycoproteins containing both significant regulation of N- and O-glycosylation were observed, hinting potential co-regulation of different types of glycosylation during tumor progress.					
37146305	0	83	theme	Intact	110:115	arg1	Glycopeptides					117:129	O-Linked Intact Glycopeptides	101:129	O-Linked Intact Glycopeptides	101:129	Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides.					
37146305	1	84	theme	cancer	383:388	arg1	discrimination					365:378	the discrimination	361:378	the discrimination of cancer	361:388	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	4	85	gly	glycopeptides	1029:1041	arg2	glycopeptides					1029:1041	O-linked intact glycopeptides	1013:1041	85.1% O-linked intact glycopeptides presented in the first fraction	1007:1073	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	7	86	theme	glycopeptides	1801:1813	arg1	characterization					1762:1777	the characterization	1758:1777	the characterization of N-/O-linked intact glycopeptides at the proteomics scale	1758:1837	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	3	87	gly	glycopeptides	788:800	arg2	glycopeptides					788:800	O-linked intact glycopeptides	772:800	O-linked intact glycopeptides	772:800	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	1	88	theme	various	240:246	arg1	diseases					248:255	various diseases	240:255	various diseases including cancer	240:272	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	1	88	theme	various	240:246	arg1	cancer					267:272	cancer	267:272	cancer	267:272	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	2	89	theme	micro-heterogeneity	404:422	arg1	nature					442:447	the micro-heterogeneity and low abundance nature	400:447	the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation	400:476	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	7	90	link	N-/O-linked	1782:1792	arg1	glycopeptides					1801:1813	N-/O-linked intact glycopeptides	1782:1813	N-/O-linked intact glycopeptides	1782:1813	In summary, this integrated platform opened a potentially useful avenue for the global analysis of protein glycosylation and can serve as a useful tool for the characterization of N-/O-linked intact glycopeptides at the proteomics scale.					
37146305	0	91	theme	Integrated	18:27	arg1	Platform					29:36	an Integrated Platform	15:36	an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides	15:129	Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides.					
37146305	3	92	dep	enrichment	730:739	arg1	the					713:715	the	713:715	the	713:715	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	2	93	theme	N-/O-linked	452:462	arg1	glycosylation					464:476	N-/O-linked glycosylation	452:476	N-/O-linked glycosylation	452:476	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	5	94	theme	intact	1375:1380	arg1	glycopeptides					1382:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	significantly changed O-linked and N-linked intact glycopeptides	1331:1394	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	5	95	theme	serum	1253:1257	arg1	samples					1259:1265	serum samples	1253:1265	serum samples	1253:1265	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	4	96	link	O-linked	1013:1020	arg1	glycopeptides					1029:1041	O-linked intact glycopeptides	1013:1041	85.1% O-linked intact glycopeptides presented in the first fraction	1007:1073	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	5	97	theme	control	1296:1302	arg1	samples					1259:1265	serum samples	1253:1265	serum samples	1253:1265	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	0	98	theme	O-Linked	101:108	arg1	Glycopeptides					117:129	O-Linked Intact Glycopeptides	101:129	O-Linked Intact Glycopeptides	101:129	Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides.					
37146305	0	99	theme	Simultaneous	46:57	arg1	Enrichment					59:68	Simultaneous Enrichment	46:68	Simultaneous Enrichment	46:68	Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides.					
37146305	6	100	gly	glycoproteins	1417:1429	arg1	glycoproteins					1417:1429	five glycoproteins	1412:1429	five glycoproteins containing both significant regulation of N- and O-glycosylation	1412:1494	Interestingly, five glycoproteins containing both significant regulation of N- and O-glycosylation were observed, hinting potential co-regulation of different types of glycosylation during tumor progress.					
37146305	4	101	theme	first	1060:1064	arg1	fraction					1066:1073	the first fraction	1056:1073	the first fraction	1056:1073	By fine-tuning the experimental conditions, we demonstrated that this platform allowed the selective separation of N- and O-linked intact glycopeptides into two fractions, with 85.1% O-linked intact glycopeptides presented in the first fraction and 93.4% N-linked intact glycopeptides presented in the second fraction.					
37146305	1	102	link	N-linked	137:144	arg1	glycosylation					146:158	N-linked glycosylation	137:158	N-linked glycosylation	137:158	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	1	103	theme	promising	336:344	arg1	biomarkers					346:355	promising biomarkers	336:355	promising biomarkers for the discrimination of cancer	336:388	Both N-linked glycosylation and O-linked glycosylation play essential roles in the onset and progression of various diseases including cancer, and N-/O-linked site-specific glycans have been proven to be promising biomarkers for the discrimination of cancer.					
37146305	3	104	theme	integrated	689:698	arg1	platform					700:707	an integrated platform	686:707	an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample	686:827	In this study, we developed an integrated platform for the simultaneous enrichment and characterization of N- and O-linked intact glycopeptides from the same serum sample.					
37146305	6	105	theme	tumor	1586:1590	arg1	progress					1592:1599	tumor progress	1586:1599	tumor progress	1586:1599	Interestingly, five glycoproteins containing both significant regulation of N- and O-glycosylation were observed, hinting potential co-regulation of different types of glycosylation during tumor progress.					
37146305	5	106	theme	health	1289:1294	arg1	control					1296:1302	health control	1289:1302	health control	1289:1302	Determined with high reproducibility, this platform was further applied to the differential analysis of serum samples of gastric cancer and health control, which revealed 17 and 181 significantly changed O-linked and N-linked intact glycopeptides.					
37146305	2	107	dep	micro-heterogeneity	404:422	arg1	the					400:402	the	400:402	the	400:402	However, the micro-heterogeneity and low abundance nature of N-/O-linked glycosylation, as well as the time-consuming and tedious procedures for the enrichment of O-linked intact glycopeptides, pose great challenges for their efficient and accurate characterization.					
37146305	6	108	theme	glycosylation	1565:1577	arg1	types					1556:1560	different types	1546:1560	different types of glycosylation	1546:1577	Interestingly, five glycoproteins containing both significant regulation of N- and O-glycosylation were observed, hinting potential co-regulation of different types of glycosylation during tumor progress.					
35593380	0	0	theme	dual	72:75	arg1	release					91:97	dual growth factor release	72:97	dual growth factor release	72:97	Development of a system of heparin multilayers on titanium surfaces for dual growth factor release.					
35593380	1	1	theme	human	310:314	arg1	protein					333:339	recombinant human bone morphogenic protein 2	298:341	recombinant human bone morphogenic protein 2 (rhBMP2)	298:350	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	1	theme	human	310:314	arg1	rhBMP2					344:349	rhBMP2	344:349	rhBMP2	344:349	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	2	from	delivery	286:293	arg1	quantities					448:457	clinically relevant quantities	428:457	clinically relevant quantities	428:457	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	7	3	theme	individual	1243:1252	arg1	composition					1254:1264	individual composition	1243:1264	individual composition	1243:1264	In summary, the system provides a modular platform for growth factor delivery that allows for individual composition and accentuation of angiogenic and osteogenic surface properties.					
35593380	4	4	from	proliferation	757:769	arg1	hMSCs					747:751	hMSCs	747:751	hMSCs	747:751	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	1	5	theme	PEM	228:230	arg1	coatings					233:240	poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings	168:240	poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings	168:240	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	5	6	theme	PEMs	916:919	arg1	architecture					896:907	architecture	896:907	architecture	896:907	Osteogenic and angiogenic effects were modified by variation in cross-linking and architecture of the PEMs.					
35593380	5	6	theme	PEMs	916:919	arg1	cross-linking					878:890	cross-linking	878:890	cross-linking	878:890	Osteogenic and angiogenic effects were modified by variation in cross-linking and architecture of the PEMs.					
35593380	1	7	theme	bone	316:319	arg1	protein					333:339	recombinant human bone morphogenic protein 2	298:341	recombinant human bone morphogenic protein 2 (rhBMP2)	298:350	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	7	theme	bone	316:319	arg1	rhBMP2					344:349	rhBMP2	344:349	rhBMP2	344:349	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	3	8	theme	stem	667:670	arg1	cells					672:676	human mesenchymal stem cells	649:676	human mesenchymal stem cells (hMSCs)	649:684	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	3	8	theme	stem	667:670	arg1	hMSCs					679:683	hMSCs	679:683	hMSCs	679:683	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	2	9	theme	Release	460:466	arg1	characteristics					468:482	Release characteristics	460:482	Release characteristics for both growth factors	460:506	Release characteristics for both growth factors differed significantly depending on film architecture.					
35593380	6	10	theme	angiogenic	1098:1107	arg1	function					1123:1130	angiogenic or osteogenic function	1098:1130	angiogenic or osteogenic function	1098:1130	By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface-activity modified to enhance angiogenic or osteogenic function in various ways.					
35593380	0	11	theme	factor	84:89	arg1	release					91:97	dual growth factor release	72:97	dual growth factor release	72:97	Development of a system of heparin multilayers on titanium surfaces for dual growth factor release.					
35593380	1	12	theme	morphogenic	321:331	arg1	protein					333:339	recombinant human bone morphogenic protein 2	298:341	recombinant human bone morphogenic protein 2 (rhBMP2)	298:350	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	12	theme	morphogenic	321:331	arg1	rhBMP2					344:349	rhBMP2	344:349	rhBMP2	344:349	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	13	theme	coatings	233:240	arg1	platform					156:163	a modular platform	146:163	a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities	146:457	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	0	14	theme	growth	77:82	arg1	release					91:97	dual growth factor release	72:97	dual growth factor release	72:97	Development of a system of heparin multilayers on titanium surfaces for dual growth factor release.					
35593380	1	15	theme	protein	333:339	arg1	delivery					286:293	dual growth factor delivery	267:293	dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities	267:457	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	7	16	theme	angiogenic	1286:1295	arg1	properties					1320:1329	angiogenic and osteogenic surface properties	1286:1329	angiogenic and osteogenic surface properties	1286:1329	In summary, the system provides a modular platform for growth factor delivery that allows for individual composition and accentuation of angiogenic and osteogenic surface properties.					
35593380	2	17	theme	growth	493:498	arg1	factors					500:506	both growth factors	488:506	both growth factors	488:506	Release characteristics for both growth factors differed significantly depending on film architecture.					
35593380	0	18	from	multilayers	35:45	arg1	surfaces					59:66	titanium surfaces	50:66	titanium surfaces	50:66	Development of a system of heparin multilayers on titanium surfaces for dual growth factor release.					
35593380	5	19	theme	angiogenic	829:838	arg1	effects					840:846	Osteogenic and angiogenic effects	814:846	Osteogenic and angiogenic effects	814:846	Osteogenic and angiogenic effects were modified by variation in cross-linking and architecture of the PEMs.					
35593380	6	20	theme	various	1135:1141	arg1	ways					1143:1146	various ways	1135:1146	various ways	1135:1146	By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface-activity modified to enhance angiogenic or osteogenic function in various ways.					
35593380	7	21	theme	growth	1204:1209	arg1	factor					1211:1216	growth factor	1204:1216	growth factor delivery	1204:1225	In summary, the system provides a modular platform for growth factor delivery that allows for individual composition and accentuation of angiogenic and osteogenic surface properties.					
35593380	6	22	theme	factor	1026:1031	arg1	delivery					1033:1040	both growth factor delivery	1014:1040	both growth factor delivery	1014:1040	By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface-activity modified to enhance angiogenic or osteogenic function in various ways.					
35593380	6	23	theme	distinct	956:963	arg1	zones					965:969	distinct zones	956:969	distinct zones	956:969	By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface-activity modified to enhance angiogenic or osteogenic function in various ways.					
35593380	6	24	theme	release	972:978	arg1	characteristics					980:994	release characteristics	972:994	release characteristics	972:994	By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface-activity modified to enhance angiogenic or osteogenic function in various ways.					
35593380	1	25	theme	modular	148:154	arg1	platform					156:163	a modular platform	146:163	a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities	146:457	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	7	26	theme	modular	1183:1189	arg1	platform					1191:1198	a modular platform	1181:1198	a modular platform for growth factor delivery that allows for individual composition and accentuation of angiogenic and osteogenic surface properties	1181:1329	In summary, the system provides a modular platform for growth factor delivery that allows for individual composition and accentuation of angiogenic and osteogenic surface properties.					
35593380	1	27	theme	titanium	245:252	arg1	surfaces					254:261	titanium surfaces	245:261	titanium surfaces	245:261	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	3	28	theme	cells	672:676	arg1	activation					578:587	activation	578:587	activation of alkaline phosphatase in C2C12 cells	578:626	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	3	28	theme	cells	672:676	arg1	proliferation					632:644	proliferation	632:644	proliferation of human mesenchymal stem cells (hMSCs)	632:684	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	1	29	theme	relevant	439:446	arg1	quantities					448:457	clinically relevant quantities	428:457	clinically relevant quantities	428:457	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	4	30	theme	factor	731:736	arg1	induction					703:711	induction	703:711	induction of von Willebrand factor (vWF) in hMSCs	703:751	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	4	30	theme	factor	731:736	arg1	proliferation					757:769	proliferation	757:769	proliferation of human umbilical vein endothelial cells	757:811	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	3	31	from	activation	578:587	arg1	cells					622:626	C2C12 cells	616:626	C2C12 cells	616:626	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	5	32	mod	modified	853:860	arg1	effects					840:846	Osteogenic and angiogenic effects	814:846	Osteogenic and angiogenic effects	814:846	Osteogenic and angiogenic effects were modified by variation in cross-linking and architecture of the PEMs.					
35593380	5	32	mod	modified	853:860	arg3	variation					865:873	variation	865:873	variation in cross-linking and architecture of the PEMs	865:919	Osteogenic and angiogenic effects were modified by variation in cross-linking and architecture of the PEMs.					
35593380	0	33	theme	system	17:22	arg1	Development					0:10	Development	0:10	Development of a system of heparin multilayers on titanium surfaces for dual growth factor release.	0:98	Development of a system of heparin multilayers on titanium surfaces for dual growth factor release.					
35593380	2	34	theme	film	544:547	arg1	architecture					549:560	film architecture	544:560	film architecture	544:560	Release characteristics for both growth factors differed significantly depending on film architecture.					
35593380	4	35	theme	cells	807:811	arg1	induction					703:711	induction	703:711	induction of von Willebrand factor (vWF) in hMSCs	703:751	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	4	35	theme	cells	807:811	arg1	proliferation					757:769	proliferation	757:769	proliferation of human umbilical vein endothelial cells	757:811	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	5	36	theme	Osteogenic	814:823	arg1	effects					840:846	Osteogenic and angiogenic effects	814:846	Osteogenic and angiogenic effects	814:846	Osteogenic and angiogenic effects were modified by variation in cross-linking and architecture of the PEMs.					
35593380	6	37	theme	growth	1019:1024	arg1	delivery					1033:1040	both growth factor delivery	1014:1040	both growth factor delivery	1014:1040	By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface-activity modified to enhance angiogenic or osteogenic function in various ways.					
35593380	3	38	theme	human	649:653	arg1	cells					672:676	human mesenchymal stem cells	649:676	human mesenchymal stem cells (hMSCs)	649:684	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	3	38	theme	human	649:653	arg1	hMSCs					679:683	hMSCs	679:683	hMSCs	679:683	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	6	39	with	films	945:949	arg1	zones					965:969	distinct zones	956:969	distinct zones	956:969	By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface-activity modified to enhance angiogenic or osteogenic function in various ways.					
35593380	1	40	theme	poly-L-lysine-heparin	168:188	arg1	coatings					233:240	poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings	168:240	poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings	168:240	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	0	41	theme	heparin	27:33	arg1	multilayers					35:45	heparin multilayers	27:45	heparin multilayers on titanium surfaces	27:66	Development of a system of heparin multilayers on titanium surfaces for dual growth factor release.					
35593380	1	42	theme	dual	267:270	arg1	factor					279:284	dual growth factor	267:284	dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities	267:457	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	3	43	theme	mesenchymal	655:665	arg1	cells					672:676	human mesenchymal stem cells	649:676	human mesenchymal stem cells (hMSCs)	649:684	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	3	43	theme	mesenchymal	655:665	arg1	hMSCs					679:683	hMSCs	679:683	hMSCs	679:683	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	3	44	theme	alkaline	592:599	arg1	phosphatase					601:611	alkaline phosphatase	592:611	alkaline phosphatase	592:611	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	7	45	theme	properties	1320:1329	arg1	accentuation					1270:1281	accentuation	1270:1281	accentuation	1270:1281	In summary, the system provides a modular platform for growth factor delivery that allows for individual composition and accentuation of angiogenic and osteogenic surface properties.					
35593380	7	45	theme	properties	1320:1329	arg1	composition					1254:1264	individual composition	1243:1264	individual composition	1243:1264	In summary, the system provides a modular platform for growth factor delivery that allows for individual composition and accentuation of angiogenic and osteogenic surface properties.					
35593380	7	46	theme	surface	1312:1318	arg1	properties					1320:1329	angiogenic and osteogenic surface properties	1286:1329	angiogenic and osteogenic surface properties	1286:1329	In summary, the system provides a modular platform for growth factor delivery that allows for individual composition and accentuation of angiogenic and osteogenic surface properties.					
35593380	1	47	theme	growth	272:277	arg1	factor					279:284	dual growth factor	267:284	dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities	267:457	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	0	48	from	surfaces	59:66	arg1	system					17:22	a system	15:22	a system of heparin multilayers on titanium surfaces for dual growth factor release	15:97	Development of a system of heparin multilayers on titanium surfaces for dual growth factor release.					
35593380	3	49	theme	phosphatase	601:611	arg1	activation					578:587	activation	578:587	activation of alkaline phosphatase in C2C12 cells	578:626	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	3	49	theme	phosphatase	601:611	arg1	proliferation					632:644	proliferation	632:644	proliferation of human mesenchymal stem cells (hMSCs)	632:684	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	1	50	theme	PLL-Hep	191:197	arg1	coatings					233:240	poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings	168:240	poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings	168:240	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	51	theme	factor	279:284	arg1	delivery					286:293	dual growth factor delivery	267:293	dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities	267:457	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	52	theme	human	368:372	arg1	rhVEGF165					414:422	rhVEGF165	414:422	rhVEGF165	414:422	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	52	theme	human	368:372	arg1	factor					402:407	recombinant human vascular endothelial growth factor 165	356:411	recombinant human vascular endothelial growth factor 165 (rhVEGF165)	356:423	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	6	53	theme	osteogenic	1112:1121	arg1	function					1123:1130	angiogenic or osteogenic function	1098:1130	angiogenic or osteogenic function	1098:1130	By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface-activity modified to enhance angiogenic or osteogenic function in various ways.					
35593380	0	54	theme	multilayers	35:45	arg1	system					17:22	a system	15:22	a system of heparin multilayers on titanium surfaces for dual growth factor release	15:97	Development of a system of heparin multilayers on titanium surfaces for dual growth factor release.					
35593380	6	55	theme	multilayer	934:943	arg1	films					945:949	multilayer films	934:949	multilayer films with distinct zones	934:969	By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface-activity modified to enhance angiogenic or osteogenic function in various ways.					
35593380	1	56	theme	recombinant	298:308	arg1	protein					333:339	recombinant human bone morphogenic protein 2	298:341	recombinant human bone morphogenic protein 2 (rhBMP2)	298:350	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	56	theme	recombinant	298:308	arg1	rhBMP2					344:349	rhBMP2	344:349	rhBMP2	344:349	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	57	theme	vascular	374:381	arg1	rhVEGF165					414:422	rhVEGF165	414:422	rhVEGF165	414:422	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	57	theme	vascular	374:381	arg1	factor					402:407	recombinant human vascular endothelial growth factor 165	356:411	recombinant human vascular endothelial growth factor 165 (rhVEGF165)	356:423	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	58	theme	recombinant	356:366	arg1	rhVEGF165					414:422	rhVEGF165	414:422	rhVEGF165	414:422	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	58	theme	recombinant	356:366	arg1	factor					402:407	recombinant human vascular endothelial growth factor 165	356:411	recombinant human vascular endothelial growth factor 165 (rhVEGF165)	356:423	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	3	59	from	proliferation	632:644	arg1	cells					622:626	C2C12 cells	616:626	C2C12 cells	616:626	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	4	60	theme	umbilical	780:788	arg1	cells					807:811	human umbilical vein endothelial cells	774:811	human umbilical vein endothelial cells	774:811	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	1	61	theme	endothelial	383:393	arg1	rhVEGF165					414:422	rhVEGF165	414:422	rhVEGF165	414:422	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	61	theme	endothelial	383:393	arg1	factor					402:407	recombinant human vascular endothelial growth factor 165	356:411	recombinant human vascular endothelial growth factor 165 (rhVEGF165)	356:423	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	4	62	theme	human	774:778	arg1	cells					807:811	human umbilical vein endothelial cells	774:811	human umbilical vein endothelial cells	774:811	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	4	63	theme	endothelial	795:805	arg1	cells					807:811	human umbilical vein endothelial cells	774:811	human umbilical vein endothelial cells	774:811	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	4	64	theme	Willebrand	720:729	arg1	vWF					739:741	vWF	739:741	vWF	739:741	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	4	64	theme	Willebrand	720:729	arg1	factor					731:736	von Willebrand factor	716:736	von Willebrand factor (vWF)	716:742	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	6	65	theme	delivery	1033:1040	arg1	proportion					1000:1009	proportion	1000:1009	proportion	1000:1009	By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface-activity modified to enhance angiogenic or osteogenic function in various ways.					
35593380	6	65	theme	delivery	1033:1040	arg1	characteristics					980:994	release characteristics	972:994	release characteristics	972:994	By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface-activity modified to enhance angiogenic or osteogenic function in various ways.					
35593380	1	66	theme	growth	395:400	arg1	rhVEGF165					414:422	rhVEGF165	414:422	rhVEGF165	414:422	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	66	theme	growth	395:400	arg1	factor					402:407	recombinant human vascular endothelial growth factor 165	356:411	recombinant human vascular endothelial growth factor 165 (rhVEGF165)	356:423	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	5	67	from	variation	865:873	arg1	architecture					896:907	architecture	896:907	architecture	896:907	Osteogenic and angiogenic effects were modified by variation in cross-linking and architecture of the PEMs.					
35593380	5	67	from	variation	865:873	arg1	cross-linking					878:890	cross-linking	878:890	cross-linking	878:890	Osteogenic and angiogenic effects were modified by variation in cross-linking and architecture of the PEMs.					
35593380	4	68	theme	von	716:718	arg1	vWF					739:741	vWF	739:741	vWF	739:741	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	4	68	theme	von	716:718	arg1	factor					731:736	von Willebrand factor	716:736	von Willebrand factor (vWF)	716:742	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	1	69	theme	polyelectrolyte	200:214	arg1	coatings					233:240	poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings	168:240	poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings	168:240	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	70	theme	factor	402:407	arg1	delivery					286:293	dual growth factor delivery	267:293	dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities	267:457	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	4	71	theme	vein	790:793	arg1	cells					807:811	human umbilical vein endothelial cells	774:811	human umbilical vein endothelial cells	774:811	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	1	72	from	platform	156:163	arg1	surfaces					254:261	titanium surfaces	245:261	titanium surfaces	245:261	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	1	73	theme	present	115:121	arg1	study					123:127	the present study	111:127	the present study	111:127	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	4	74	from	induction	703:711	arg1	hMSCs					747:751	hMSCs	747:751	hMSCs	747:751	rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells.					
35593380	1	75	theme	multilayer	216:225	arg1	coatings					233:240	poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings	168:240	poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings	168:240	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	0	76	theme	titanium	50:57	arg1	surfaces					59:66	titanium surfaces	50:66	titanium surfaces	50:66	Development of a system of heparin multilayers on titanium surfaces for dual growth factor release.					
35593380	0	77	from	system	17:22	arg1	surfaces					59:66	titanium surfaces	50:66	titanium surfaces	50:66	Development of a system of heparin multilayers on titanium surfaces for dual growth factor release.					
35593380	3	78	theme	C2C12	616:620	arg1	cells					622:626	C2C12 cells	616:626	C2C12 cells	616:626	rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs).					
35593380	1	79	theme	study	123:127	arg1	aim					104:106	The aim	100:106	The aim of the present study	100:127	The aim of the present study was to establish a modular platform of poly-L-lysine-heparin (PLL-Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities.					
35593380	7	80	theme	osteogenic	1301:1310	arg1	properties					1320:1329	angiogenic and osteogenic surface properties	1286:1329	angiogenic and osteogenic surface properties	1286:1329	In summary, the system provides a modular platform for growth factor delivery that allows for individual composition and accentuation of angiogenic and osteogenic surface properties.					
35593380	7	81	theme	factor	1211:1216	arg1	delivery					1218:1225	growth factor delivery	1204:1225	growth factor delivery	1204:1225	In summary, the system provides a modular platform for growth factor delivery that allows for individual composition and accentuation of angiogenic and osteogenic surface properties.					
35301159	3	0	theme	pith	318:321	arg1	TPE					332:334	TPE	332:334	TPE	332:334	We hypothesized that tangerine pith extract (TPE) has prebiotic effects on gut dysbiosis in antibiotic-treated mice.					
35301159	3	0	theme	pith	318:321	arg1	extract					323:329	tangerine pith extract	308:329	tangerine pith extract (TPE)	308:335	We hypothesized that tangerine pith extract (TPE) has prebiotic effects on gut dysbiosis in antibiotic-treated mice.					
35301159	12	1	theme	gut	1485:1487	arg1	microbiota					1489:1498	the gut microbiota	1481:1498	the gut microbiota in mice	1481:1506	In conclusion, TPE is effective in restoring the gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.					
35301159	5	2	theme	antibiotic	536:545	arg1	cocktail					547:554	antibiotic cocktail	536:554	antibiotic cocktail	536:554	Gut dysbiosis was induced through treatment with antibiotic cocktail for 7 days in mice.					
35301159	11	3	theme	intestinal	1380:1389	arg1	occludin					1391:1398	intestinal occludin	1380:1398	intestinal occludin	1380:1398	Moreover, protein expression of intestinal occludin was significantly increased by TPE.					
35301159	9	4	theme	colonic	1141:1147	arg1	contents					1149:1156	the colonic contents	1137:1156	the colonic contents	1137:1156	Furthermore, the production of propionate and butyrate in the colonic contents were significantly enhanced in the TPE-treated mice.					
35301159	11	5	theme	occludin	1391:1398	arg1	expression					1366:1375	protein expression	1358:1375	protein expression of intestinal occludin	1358:1398	Moreover, protein expression of intestinal occludin was significantly increased by TPE.					
35301159	1	6	theme	other	141:145	arg1	functions					161:169	other physiological functions	141:169	other physiological functions	141:169	The gut microbiota can affect the intestinal barrier and other physiological functions.					
35301159	10	7	theme	treatment	1293:1301	arg1	group					1303:1307	the antibiotic treatment group	1278:1307	the antibiotic treatment group	1278:1307	Serum D-lactic acid and diamine oxidase activity were increased in the antibiotic treatment group and reversed in the TPE-treated group.					
35301159	10	8	theme	oxidase	1243:1249	arg1	activity					1251:1258	diamine oxidase activity	1235:1258	diamine oxidase activity	1235:1258	Serum D-lactic acid and diamine oxidase activity were increased in the antibiotic treatment group and reversed in the TPE-treated group.					
35301159	3	9	theme	prebiotic	341:349	arg1	effects					351:357	prebiotic effects	341:357	prebiotic effects	341:357	We hypothesized that tangerine pith extract (TPE) has prebiotic effects on gut dysbiosis in antibiotic-treated mice.					
35301159	10	10	theme	antibiotic	1282:1291	arg1	group					1303:1307	the antibiotic treatment group	1278:1307	the antibiotic treatment group	1278:1307	Serum D-lactic acid and diamine oxidase activity were increased in the antibiotic treatment group and reversed in the TPE-treated group.					
35301159	10	11	theme	diamine	1235:1241	arg1	oxidase					1243:1249	diamine oxidase	1235:1249	diamine oxidase activity	1235:1258	Serum D-lactic acid and diamine oxidase activity were increased in the antibiotic treatment group and reversed in the TPE-treated group.					
35301159	4	12	theme	ultrafiltration	463:477	arg1	method					479:484	an ultrafiltration method	460:484	an ultrafiltration method	460:484	TPE was prepared via acid hydrolysis and isolated using an ultrafiltration method.					
35301159	8	13	theme	TPE-supplemented	1056:1071	arg1	mice					1073:1076	TPE-supplemented mice	1056:1076	TPE-supplemented mice	1056:1076	The relative abundance of some probiotics such as [Ruminococcus]_torques_group, [Clostridium]_innocuum_group, and Enterorhabdus were significantly increased and harmful bacteria such as Alistipes were decreased in TPE-supplemented mice.					
35301159	5	14	theme	Gut	487:489	arg1	dysbiosis					491:499	Gut dysbiosis	487:499	Gut dysbiosis	487:499	Gut dysbiosis was induced through treatment with antibiotic cocktail for 7 days in mice.					
35301159	12	15	theme	combatting	1565:1574	arg1	dysbiosis					1580:1588	combatting gut dysbiosis	1565:1588	combatting gut dysbiosis	1565:1588	In conclusion, TPE is effective in restoring the gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.					
35301159	8	16	theme	probiotics	873:882	arg1	abundance					855:863	The relative abundance	842:863	The relative abundance of some probiotics such as [Ruminococcus]_torques_group, [Clostridium]_innocuum_group, and Enterorhabdus	842:968	The relative abundance of some probiotics such as [Ruminococcus]_torques_group, [Clostridium]_innocuum_group, and Enterorhabdus were significantly increased and harmful bacteria such as Alistipes were decreased in TPE-supplemented mice.					
35301159	10	17	theme	TPE-treated	1329:1339	arg1	group					1341:1345	the TPE-treated group	1325:1345	the TPE-treated group	1325:1345	Serum D-lactic acid and diamine oxidase activity were increased in the antibiotic treatment group and reversed in the TPE-treated group.					
35301159	5	18	from	days	562:565	arg1	mice					570:573	mice	570:573	mice	570:573	Gut dysbiosis was induced through treatment with antibiotic cocktail for 7 days in mice.					
35301159	2	19	theme	structure-dependent	259:277	arg1	manner					279:284	a structure-dependent manner	257:284	a structure-dependent manner	257:284	Tangerine pith contains various citrus pectins, which modulate the gut microbiota in a structure-dependent manner.					
35301159	6	20	theme	other	689:693	arg1	parameters					695:704	other parameters	689:704	other parameters	689:704	After spontaneous recovery or being supplemented with 0.5 mL TPE for 14 days, the gut microbiota composition and other parameters were examined.					
35301159	1	21	theme	physiological	147:159	arg1	functions					161:169	other physiological functions	141:169	other physiological functions	141:169	The gut microbiota can affect the intestinal barrier and other physiological functions.					
35301159	10	22	theme	D-lactic	1217:1224	arg1	acid					1226:1229	Serum D-lactic acid	1211:1229	Serum D-lactic acid	1211:1229	Serum D-lactic acid and diamine oxidase activity were increased in the antibiotic treatment group and reversed in the TPE-treated group.					
35301159	3	23	from	dysbiosis	366:374	arg1	mice					398:401	antibiotic-treated mice	379:401	antibiotic-treated mice	379:401	We hypothesized that tangerine pith extract (TPE) has prebiotic effects on gut dysbiosis in antibiotic-treated mice.					
35301159	7	24	theme	ribosomal	729:737	arg1	sequencing					759:768	The 16s ribosomal DNA high-throughput sequencing	721:768	The 16s ribosomal DNA high-throughput sequencing	721:768	The 16s ribosomal DNA high-throughput sequencing and analysis confirmed that TPE positively improved the gut microbiota.					
35301159	9	25	theme	propionate	1110:1119	arg1	production					1096:1105	the production	1092:1105	the production of propionate and butyrate in the colonic contents	1092:1156	Furthermore, the production of propionate and butyrate in the colonic contents were significantly enhanced in the TPE-treated mice.					
35301159	12	26	from	conclusion	1439:1448	arg1	effective					1458:1466	effective	1458:1466	effective	1458:1466	In conclusion, TPE is effective in restoring the gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.					
35301159	0	27	theme	Gut	0:2	arg1	dysbiosis					4:12	Gut dysbiosis	0:12	Gut dysbiosis induced by antibiotics	0:35	Gut dysbiosis induced by antibiotics is improved by tangerine pith extract in mice.					
35301159	12	28	from	prebiotic	1552:1560	arg1	dysbiosis					1580:1588	combatting gut dysbiosis	1565:1588	combatting gut dysbiosis	1565:1588	In conclusion, TPE is effective in restoring the gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.					
35301159	12	29	theme	antibiotic	1514:1523	arg1	treatment					1525:1533	antibiotic treatment	1514:1533	antibiotic treatment	1514:1533	In conclusion, TPE is effective in restoring the gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.					
35301159	6	30	theme	microbiota	662:671	arg1	composition					673:683	the gut microbiota composition	654:683	the gut microbiota composition	654:683	After spontaneous recovery or being supplemented with 0.5 mL TPE for 14 days, the gut microbiota composition and other parameters were examined.					
35301159	11	31	theme	protein	1358:1364	arg1	expression					1366:1375	protein expression	1358:1375	protein expression of intestinal occludin	1358:1398	Moreover, protein expression of intestinal occludin was significantly increased by TPE.					
35301159	9	32	from	production	1096:1105	arg1	contents					1149:1156	the colonic contents	1137:1156	the colonic contents	1137:1156	Furthermore, the production of propionate and butyrate in the colonic contents were significantly enhanced in the TPE-treated mice.					
35301159	8	33	theme	harmful	1003:1009	arg1	bacteria					1011:1018	harmful bacteria	1003:1018	harmful bacteria such as Alistipes	1003:1036	The relative abundance of some probiotics such as [Ruminococcus]_torques_group, [Clostridium]_innocuum_group, and Enterorhabdus were significantly increased and harmful bacteria such as Alistipes were decreased in TPE-supplemented mice.					
35301159	8	33	theme	harmful	1003:1009	arg1	Alistipes					1028:1036	Alistipes	1028:1036	Alistipes	1028:1036	The relative abundance of some probiotics such as [Ruminococcus]_torques_group, [Clostridium]_innocuum_group, and Enterorhabdus were significantly increased and harmful bacteria such as Alistipes were decreased in TPE-supplemented mice.					
35301159	6	34	theme	gut	658:660	arg1	composition					673:683	the gut microbiota composition	654:683	the gut microbiota composition	654:683	After spontaneous recovery or being supplemented with 0.5 mL TPE for 14 days, the gut microbiota composition and other parameters were examined.					
35301159	3	35	theme	gut	362:364	arg1	dysbiosis					366:374	gut dysbiosis	362:374	gut dysbiosis in antibiotic-treated mice	362:401	We hypothesized that tangerine pith extract (TPE) has prebiotic effects on gut dysbiosis in antibiotic-treated mice.					
35301159	2	36	theme	Tangerine	172:180	arg1	pith					182:185	Tangerine pith	172:185	Tangerine pith	172:185	Tangerine pith contains various citrus pectins, which modulate the gut microbiota in a structure-dependent manner.					
35301159	1	37	theme	gut	88:90	arg1	microbiota					92:101	The gut microbiota	84:101	The gut microbiota	84:101	The gut microbiota can affect the intestinal barrier and other physiological functions.					
35301159	2	38	contain	contains	187:194	arg2	pectins					211:217	various citrus pectins	196:217	various citrus pectins	196:217	Tangerine pith contains various citrus pectins, which modulate the gut microbiota in a structure-dependent manner.					
35301159	2	38	contain	contains	187:194	arg1	pith					182:185	Tangerine pith	172:185	Tangerine pith	172:185	Tangerine pith contains various citrus pectins, which modulate the gut microbiota in a structure-dependent manner.					
35301159	0	39	from	extract	67:73	arg1	mice					78:81	mice	78:81	mice	78:81	Gut dysbiosis induced by antibiotics is improved by tangerine pith extract in mice.					
35301159	7	40	theme	gut	826:828	arg1	microbiota					830:839	the gut microbiota	822:839	the gut microbiota	822:839	The 16s ribosomal DNA high-throughput sequencing and analysis confirmed that TPE positively improved the gut microbiota.					
35301159	10	41	theme	Serum	1211:1215	arg1	acid					1226:1229	Serum D-lactic acid	1211:1229	Serum D-lactic acid	1211:1229	Serum D-lactic acid and diamine oxidase activity were increased in the antibiotic treatment group and reversed in the TPE-treated group.					
35301159	3	42	contain	has	337:339	arg2	effects					351:357	prebiotic effects	341:357	prebiotic effects	341:357	We hypothesized that tangerine pith extract (TPE) has prebiotic effects on gut dysbiosis in antibiotic-treated mice.					
35301159	3	42	contain	has	337:339	arg1	TPE					332:334	TPE	332:334	TPE	332:334	We hypothesized that tangerine pith extract (TPE) has prebiotic effects on gut dysbiosis in antibiotic-treated mice.					
35301159	3	42	contain	has	337:339	arg1	extract					323:329	tangerine pith extract	308:329	tangerine pith extract (TPE)	308:335	We hypothesized that tangerine pith extract (TPE) has prebiotic effects on gut dysbiosis in antibiotic-treated mice.					
35301159	12	43	from	effective	1458:1466	arg1	conclusion					1439:1448	conclusion	1439:1448	conclusion	1439:1448	In conclusion, TPE is effective in restoring the gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.					
35301159	3	44	theme	antibiotic-treated	379:396	arg1	mice					398:401	antibiotic-treated mice	379:401	antibiotic-treated mice	379:401	We hypothesized that tangerine pith extract (TPE) has prebiotic effects on gut dysbiosis in antibiotic-treated mice.					
35301159	12	45	theme	gut	1576:1578	arg1	dysbiosis					1580:1588	combatting gut dysbiosis	1565:1588	combatting gut dysbiosis	1565:1588	In conclusion, TPE is effective in restoring the gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.					
35301159	3	46	theme	tangerine	308:316	arg1	TPE					332:334	TPE	332:334	TPE	332:334	We hypothesized that tangerine pith extract (TPE) has prebiotic effects on gut dysbiosis in antibiotic-treated mice.					
35301159	3	46	theme	tangerine	308:316	arg1	extract					323:329	tangerine pith extract	308:329	tangerine pith extract (TPE)	308:335	We hypothesized that tangerine pith extract (TPE) has prebiotic effects on gut dysbiosis in antibiotic-treated mice.					
35301159	2	47	theme	citrus	204:209	arg1	pectins					211:217	various citrus pectins	196:217	various citrus pectins	196:217	Tangerine pith contains various citrus pectins, which modulate the gut microbiota in a structure-dependent manner.					
35301159	12	48	from	microbiota	1489:1498	arg1	mice					1503:1506	mice	1503:1506	mice	1503:1506	In conclusion, TPE is effective in restoring the gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.					
35301159	2	49	theme	various	196:202	arg1	pectins					211:217	various citrus pectins	196:217	various citrus pectins	196:217	Tangerine pith contains various citrus pectins, which modulate the gut microbiota in a structure-dependent manner.					
35301159	9	50	theme	butyrate	1125:1132	arg1	production					1096:1105	the production	1092:1105	the production of propionate and butyrate in the colonic contents	1092:1156	Furthermore, the production of propionate and butyrate in the colonic contents were significantly enhanced in the TPE-treated mice.					
35301159	7	51	theme	DNA	739:741	arg1	sequencing					759:768	The 16s ribosomal DNA high-throughput sequencing	721:768	The 16s ribosomal DNA high-throughput sequencing	721:768	The 16s ribosomal DNA high-throughput sequencing and analysis confirmed that TPE positively improved the gut microbiota.					
35301159	4	52	theme	acid	425:428	arg1	hydrolysis					430:439	acid hydrolysis	425:439	acid hydrolysis	425:439	TPE was prepared via acid hydrolysis and isolated using an ultrafiltration method.					
35301159	7	53	theme	high-throughput	743:757	arg1	sequencing					759:768	The 16s ribosomal DNA high-throughput sequencing	721:768	The 16s ribosomal DNA high-throughput sequencing	721:768	The 16s ribosomal DNA high-throughput sequencing and analysis confirmed that TPE positively improved the gut microbiota.					
35301159	0	54	theme	pith	62:65	arg1	extract					67:73	tangerine pith extract	52:73	tangerine pith extract in mice	52:81	Gut dysbiosis induced by antibiotics is improved by tangerine pith extract in mice.					
35301159	2	55	theme	gut	239:241	arg1	microbiota					243:252	the gut microbiota	235:252	the gut microbiota	235:252	Tangerine pith contains various citrus pectins, which modulate the gut microbiota in a structure-dependent manner.					
35301159	1	56	theme	intestinal	118:127	arg1	barrier					129:135	the intestinal barrier	114:135	the intestinal barrier	114:135	The gut microbiota can affect the intestinal barrier and other physiological functions.					
35301159	6	57	theme	TPE	637:639	arg1	mL					634:635	0.5 mL TPE	630:639	0.5 mL TPE for 14 days	630:651	After spontaneous recovery or being supplemented with 0.5 mL TPE for 14 days, the gut microbiota composition and other parameters were examined.					
35301159	7	58	theme	16s	725:727	arg1	sequencing					759:768	The 16s ribosomal DNA high-throughput sequencing	721:768	The 16s ribosomal DNA high-throughput sequencing	721:768	The 16s ribosomal DNA high-throughput sequencing and analysis confirmed that TPE positively improved the gut microbiota.					
35301159	0	59	theme	tangerine	52:60	arg1	extract					67:73	tangerine pith extract	52:73	tangerine pith extract in mice	52:81	Gut dysbiosis induced by antibiotics is improved by tangerine pith extract in mice.					
35301159	6	60	theme	spontaneous	582:592	arg1	recovery					594:601	spontaneous recovery	582:601	spontaneous recovery	582:601	After spontaneous recovery or being supplemented with 0.5 mL TPE for 14 days, the gut microbiota composition and other parameters were examined.					
35301159	9	61	theme	TPE-treated	1193:1203	arg1	mice					1205:1208	the TPE-treated mice	1189:1208	the TPE-treated mice	1189:1208	Furthermore, the production of propionate and butyrate in the colonic contents were significantly enhanced in the TPE-treated mice.					
35301159	8	62	theme	relative	846:853	arg1	abundance					855:863	The relative abundance	842:863	The relative abundance of some probiotics such as [Ruminococcus]_torques_group, [Clostridium]_innocuum_group, and Enterorhabdus	842:968	The relative abundance of some probiotics such as [Ruminococcus]_torques_group, [Clostridium]_innocuum_group, and Enterorhabdus were significantly increased and harmful bacteria such as Alistipes were decreased in TPE-supplemented mice.					
35301159	5	63	with	treatment	521:529	arg1	cocktail					547:554	antibiotic cocktail	536:554	antibiotic cocktail	536:554	Gut dysbiosis was induced through treatment with antibiotic cocktail for 7 days in mice.					
36876765	0	0	from	enzymes	33:39	arg1	biosynthesis					60:71	heparan sulfate biosynthesis	44:71	heparan sulfate biosynthesis	44:71	Interacting polymer-modification enzymes in heparan sulfate biosynthesis.					
36876765	4	1	theme	IdoA	726:729	arg1	ratios					735:740	GlcA and IdoA D/H ratios	717:740	GlcA and IdoA D/H ratios related to product composition	717:771	GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions.					
36876765	2	2	theme	hexuronyl	474:482	arg1	Hs2st					506:510	Hs2st	506:510	Hs2st	506:510	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	2	2	theme	hexuronyl	474:482	arg1	2-O-sulfotransferase					484:503	hexuronyl 2-O-sulfotransferase	474:503	hexuronyl 2-O-sulfotransferase (Hs2st)	474:511	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	2	3	theme	recombinant	338:348	arg1	enzymes					350:356	recombinant enzymes	338:356	recombinant enzymes in a D2O/H2O medium	338:376	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	5	4	theme	Hsepi/Hs6st	934:944	arg1	complex					946:952	a functional Hsepi/Hs6st complex	921:952	a functional Hsepi/Hs6st complex	921:952	Evidence for a functional Hsepi/Hs6st complex was provided by selective incorporation of D atoms into GlcA units adjacent to 6-O-sulfated glucosamine residues.					
36876765	7	5	theme	heparan	1273:1279	arg1	biosynthesis					1289:1300	heparan sulfate biosynthesis	1273:1300	heparan sulfate biosynthesis	1273:1300	These findings provide novel insight into the roles of enzyme interactions in heparan sulfate biosynthesis.					
36876765	5	6	theme	glucosamine	1046:1056	arg1	residues					1058:1065	6-O-sulfated glucosamine residues	1033:1065	6-O-sulfated glucosamine residues	1033:1065	Evidence for a functional Hsepi/Hs6st complex was provided by selective incorporation of D atoms into GlcA units adjacent to 6-O-sulfated glucosamine residues.					
36876765	2	7	theme	polymer-modification	587:606	arg1	steps					608:612	the final polymer-modification steps	577:612	the final polymer-modification steps	577:612	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	1	8	theme	Glucuronyl	74:83	arg1	Hsepi					98:102	Hsepi	98:102	Hsepi	98:102	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
36876765	1	8	theme	Glucuronyl	74:83	arg1	5-epimerase					85:95	Glucuronyl 5-epimerase	74:95	Glucuronyl 5-epimerase (Hsepi)	74:103	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
36876765	2	9	theme	glucosaminyl	517:528	arg1	Hs6st					552:556	Hs6st	552:556	Hs6st	552:556	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	2	9	theme	glucosaminyl	517:528	arg1	6-O-sulfotransferase					530:549	glucosaminyl 6-O-sulfotransferase	517:549	glucosaminyl 6-O-sulfotransferase (Hs6st)	517:557	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	2	10	theme	final	581:585	arg1	steps					608:612	the final polymer-modification steps	577:612	the final polymer-modification steps	577:612	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	4	11	theme	isotope	790:796	arg1	effects					798:804	kinetic isotope effects	782:804	kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions	782:905	GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions.					
36876765	1	12	theme	hexuronic	248:256	arg1	residues					263:270	hexuronic acid residues	248:270	hexuronic acid residues	248:270	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
36876765	7	13	theme	interactions	1257:1268	arg1	roles					1241:1245	the roles	1237:1245	the roles of enzyme interactions in heparan sulfate biosynthesis	1237:1300	These findings provide novel insight into the roles of enzyme interactions in heparan sulfate biosynthesis.					
36876765	7	14	from	roles	1241:1245	arg1	biosynthesis					1289:1300	heparan sulfate biosynthesis	1273:1300	heparan sulfate biosynthesis	1273:1300	These findings provide novel insight into the roles of enzyme interactions in heparan sulfate biosynthesis.					
36876765	5	15	theme	adjacent	1021:1028	arg1	units					1015:1019	GlcA units	1010:1019	GlcA units adjacent to 6-O-sulfated glucosamine residues	1010:1065	Evidence for a functional Hsepi/Hs6st complex was provided by selective incorporation of D atoms into GlcA units adjacent to 6-O-sulfated glucosamine residues.					
36876765	7	16	theme	novel	1218:1222	arg1	insight					1224:1230	novel insight	1218:1230	novel insight into the roles of enzyme interactions in heparan sulfate biosynthesis	1218:1300	These findings provide novel insight into the roles of enzyme interactions in heparan sulfate biosynthesis.					
36876765	4	17	theme	epimerase	866:874	arg1	reactions					897:905	the coupled epimerase and sulfotransferase reactions	854:905	the coupled epimerase and sulfotransferase reactions	854:905	GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions.					
36876765	2	18	from	enzymes	350:356	arg1	medium					371:376	a D2O/H2O medium	361:376	a D2O/H2O medium	361:376	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	2	19	theme	functional	436:445	arg1	interactions					447:458	functional interactions	436:458	functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st)	436:557	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	6	20	theme	separated	1162:1170	arg1	reactions					1172:1180	topologically separated reactions	1148:1180	topologically separated reactions	1148:1180	The inability to achieve simultaneous 2-O- and 6-O-sulfation in vitro supported topologically separated reactions in the cell.					
36876765	0	21	theme	polymer-modification	12:31	arg1	enzymes					33:39	polymer-modification enzymes	12:39	polymer-modification enzymes in heparan sulfate biosynthesis	12:71	Interacting polymer-modification enzymes in heparan sulfate biosynthesis.					
36876765	4	22	theme	reactions	897:905	arg1	efficiency					840:849	efficiency	840:849	efficiency of the coupled epimerase and sulfotransferase reactions	840:905	GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions.					
36876765	5	23	theme	atoms	999:1003	arg1	incorporation					980:992	selective incorporation	970:992	selective incorporation of D atoms into GlcA units adjacent to 6-O-sulfated glucosamine residues	970:1065	Evidence for a functional Hsepi/Hs6st complex was provided by selective incorporation of D atoms into GlcA units adjacent to 6-O-sulfated glucosamine residues.					
36876765	7	24	theme	enzyme	1250:1255	arg1	interactions					1257:1268	enzyme interactions	1250:1268	enzyme interactions	1250:1268	These findings provide novel insight into the roles of enzyme interactions in heparan sulfate biosynthesis.					
36876765	1	25	theme	acid	258:261	arg1	residues					263:270	hexuronic acid residues	248:270	hexuronic acid residues	248:270	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
36876765	4	26	theme	related	742:748	arg1	ratios					735:740	GlcA and IdoA D/H ratios	717:740	GlcA and IdoA D/H ratios related to product composition	717:771	GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions.					
36876765	2	27	theme	[4GlcAβ1-4GlcNSO3α1-	290:309	arg1	substrate					323:331	a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate	288:331	a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate	288:331	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	2	28	theme	D2O/H2O	363:369	arg1	medium					371:376	a D2O/H2O medium	361:376	a D2O/H2O medium	361:376	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	1	29	theme	residues	263:270	arg1	C5					242:243	C5	242:243	C5 of hexuronic acid residues	242:270	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
36876765	1	30	theme	D-glucuronic	114:125	arg1	GlcA					133:136	GlcA	133:136	GlcA	133:136	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
36876765	1	30	theme	D-glucuronic	114:125	arg1	acid					127:130	D-glucuronic acid	114:130	D-glucuronic acid (GlcA)	114:137	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
36876765	4	31	theme	product	753:759	arg1	composition					761:771	product composition	753:771	product composition	753:771	GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions.					
36876765	5	32	theme	D	997:997	arg1	atoms					999:1003	D atoms	997:1003	D atoms	997:1003	Evidence for a functional Hsepi/Hs6st complex was provided by selective incorporation of D atoms into GlcA units adjacent to 6-O-sulfated glucosamine residues.					
36876765	2	33	theme	Hsepi	463:467	arg1	interactions					447:458	functional interactions	436:458	functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st)	436:557	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	4	34	theme	GlcA	717:720	arg1	ratios					735:740	GlcA and IdoA D/H ratios	717:740	GlcA and IdoA D/H ratios related to product composition	717:771	GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions.					
36876765	0	35	theme	sulfate	52:58	arg1	biosynthesis					60:71	heparan sulfate biosynthesis	44:71	heparan sulfate biosynthesis	44:71	Interacting polymer-modification enzymes in heparan sulfate biosynthesis.					
36876765	4	36	theme	D/H	731:733	arg1	ratios					735:740	GlcA and IdoA D/H ratios	717:740	GlcA and IdoA D/H ratios related to product composition	717:771	GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions.					
36876765	2	37	theme	exchange	397:404	arg1	approach					406:413	an isotope exchange approach	386:413	an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st)	386:557	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	0	38	theme	heparan	44:50	arg1	biosynthesis					60:71	heparan sulfate biosynthesis	44:71	heparan sulfate biosynthesis	44:71	Interacting polymer-modification enzymes in heparan sulfate biosynthesis.					
36876765	1	39	from	C5	242:243	arg1	abstraction					215:225	reversible abstraction	204:225	reversible abstraction of a proton at C5 of hexuronic acid residues	204:270	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
36876765	4	40	theme	efficiency	840:849	arg1	terms					831:835	terms	831:835	terms of efficiency of the coupled epimerase and sulfotransferase reactions	831:905	GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions.					
36876765	1	41	theme	reversible	204:213	arg1	abstraction					215:225	reversible abstraction	204:225	reversible abstraction of a proton at C5 of hexuronic acid residues	204:270	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
36876765	5	42	theme	functional	923:932	arg1	complex					946:952	a functional Hsepi/Hs6st complex	921:952	a functional Hsepi/Hs6st complex	921:952	Evidence for a functional Hsepi/Hs6st complex was provided by selective incorporation of D atoms into GlcA units adjacent to 6-O-sulfated glucosamine residues.					
36876765	6	43	theme	simultaneous	1093:1104	arg1	2-O-					1106:1109	simultaneous 2-O-	1093:1109	simultaneous 2-O-	1093:1109	The inability to achieve simultaneous 2-O- and 6-O-sulfation in vitro supported topologically separated reactions in the cell.					
36876765	3	44	theme	computational	650:662	arg1	modeling					664:671	computational modeling	650:671	computational modeling	650:671	Enzyme complexes were supported by computational modeling and homogeneous time resolved fluorescence.					
36876765	4	45	theme	coupled	858:864	arg1	reactions					897:905	the coupled epimerase and sulfotransferase reactions	854:905	the coupled epimerase and sulfotransferase reactions	854:905	GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions.					
36876765	4	46	theme	sulfotransferase	880:895	arg1	reactions					897:905	the coupled epimerase and sulfotransferase reactions	854:905	the coupled epimerase and sulfotransferase reactions	854:905	GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions.					
36876765	2	47	theme	interactions	447:458	arg1	assessment					422:431	the assessment	418:431	the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st)	418:557	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	7	48	theme	sulfate	1281:1287	arg1	biosynthesis					1289:1300	heparan sulfate biosynthesis	1273:1300	heparan sulfate biosynthesis	1273:1300	These findings provide novel insight into the roles of enzyme interactions in heparan sulfate biosynthesis.					
36876765	2	49	theme	substrate	323:331	arg1	Incubations					273:283	Incubations	273:283	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium	273:376	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	5	50	theme	6-O-sulfated	1033:1044	arg1	residues					1058:1065	6-O-sulfated glucosamine residues	1033:1065	6-O-sulfated glucosamine residues	1033:1065	Evidence for a functional Hsepi/Hs6st complex was provided by selective incorporation of D atoms into GlcA units adjacent to 6-O-sulfated glucosamine residues.					
36876765	2	51	theme	precursor	313:321	arg1	substrate					323:331	a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate	288:331	a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate	288:331	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	4	52	theme	kinetic	782:788	arg1	effects					798:804	kinetic isotope effects	782:804	kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions	782:905	GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions.					
36876765	3	53	theme	homogeneous	677:687	arg1	time					689:692	homogeneous time	677:692	homogeneous time resolved fluorescence	677:714	Enzyme complexes were supported by computational modeling and homogeneous time resolved fluorescence.					
36876765	5	54	theme	GlcA	1010:1013	arg1	units					1015:1019	GlcA units	1010:1019	GlcA units adjacent to 6-O-sulfated glucosamine residues	1010:1065	Evidence for a functional Hsepi/Hs6st complex was provided by selective incorporation of D atoms into GlcA units adjacent to 6-O-sulfated glucosamine residues.					
36876765	2	55	theme	n	311:311	arg1	substrate					323:331	a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate	288:331	a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate	288:331	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	5	56	theme	selective	970:978	arg1	incorporation					980:992	selective incorporation	970:992	selective incorporation of D atoms into GlcA units adjacent to 6-O-sulfated glucosamine residues	970:1065	Evidence for a functional Hsepi/Hs6st complex was provided by selective incorporation of D atoms into GlcA units adjacent to 6-O-sulfated glucosamine residues.					
36876765	2	57	theme	isotope	389:395	arg1	approach					406:413	an isotope exchange approach	386:413	an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st)	386:557	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	3	58	theme	Enzyme	615:620	arg1	complexes					622:630	Enzyme complexes	615:630	Enzyme complexes	615:630	Enzyme complexes were supported by computational modeling and homogeneous time resolved fluorescence.					
36876765	2	59	with	interactions	447:458	arg1	Hs2st					506:510	Hs2st	506:510	Hs2st	506:510	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	2	59	with	interactions	447:458	arg1	Hs6st					552:556	Hs6st	552:556	Hs6st	552:556	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	2	59	with	interactions	447:458	arg1	2-O-sulfotransferase					484:503	hexuronyl 2-O-sulfotransferase	474:503	hexuronyl 2-O-sulfotransferase (Hs2st)	474:511	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	2	59	with	interactions	447:458	arg1	6-O-sulfotransferase					530:549	glucosaminyl 6-O-sulfotransferase	517:549	glucosaminyl 6-O-sulfotransferase (Hs6st)	517:557	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	1	60	theme	L-iduronic	144:153	arg1	acid					155:158	L-iduronic acid	144:158	L-iduronic acid (IdoA) units	144:171	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
36876765	1	60	theme	L-iduronic	144:153	arg1	IdoA					161:164	IdoA	161:164	IdoA	161:164	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
36876765	2	61	with	Incubations	273:283	arg1	enzymes					350:356	recombinant enzymes	338:356	recombinant enzymes in a D2O/H2O medium	338:376	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	2	62	dep	enabled	378:384	arg1	both					560:563	both	560:563	both	560:563	Incubations of a [4GlcAβ1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps.					
36876765	1	63	theme	acid	155:158	arg1	units					167:171	L-iduronic acid (IdoA) units	144:171	L-iduronic acid (IdoA) units	144:171	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
36876765	1	64	theme	proton	232:237	arg1	abstraction					215:225	reversible abstraction	204:225	reversible abstraction of a proton at C5 of hexuronic acid residues	204:270	Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues.					
35651000	12	0	theme	outbreak	2646:2653	arg1	investigations					2655:2668	outbreak investigations	2646:2668	outbreak investigations	2646:2668	Investigating inter-strain interactions during growth in/on different substrates, which may have undergone temperature abuse during their transport along the supply chain or during storage in household refrigerators, could assist in explaining the mismatch between clinical and food samples during outbreak investigations.					
35651000	9	1	theme	in/on	1760:1764	arg1	Ricotta					1766:1772	more pronounced in/on Ricotta	1744:1772	more pronounced in/on Ricotta	1744:1772	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	9	1	theme	in/on	1760:1764	arg1	phenomenon					1729:1738	The phenomenon	1725:1738	The phenomenon	1725:1738	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	3	2	theme	availability	518:529	arg1	effect					501:506	the effect	497:506	the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems	497:676	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
35651000	7	3	dep	in/on	1403:1407	arg1	substrate					1418:1426	the same substrate	1409:1426	the pathogen in/on the same substrate	1390:1426	The simultaneously presence of different strains of the pathogen in/on the same substrate did not affect neither the duration of the lag phase nor the growth rate of the co-cultured strains.					
35651000	7	4	theme	strains	1379:1385	arg1	presence					1357:1364	The simultaneously presence	1338:1364	The simultaneously presence of different strains of the pathogen in/on the same substrate	1338:1426	The simultaneously presence of different strains of the pathogen in/on the same substrate did not affect neither the duration of the lag phase nor the growth rate of the co-cultured strains.					
35651000	12	5	theme	different	2408:2416	arg1	substrates					2418:2427	different substrates	2408:2427	different substrates	2408:2427	Investigating inter-strain interactions during growth in/on different substrates, which may have undergone temperature abuse during their transport along the supply chain or during storage in household refrigerators, could assist in explaining the mismatch between clinical and food samples during outbreak investigations.					
35651000	8	6	theme	population	1596:1605	arg1	reached/decrease					1607:1622	the final population reached/decrease	1586:1622	the final population reached/decrease during storage	1586:1637	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	12	7	theme	growth	2395:2400	arg1	in/on					2402:2406	growth in/on	2395:2406	growth in/on	2395:2406	Investigating inter-strain interactions during growth in/on different substrates, which may have undergone temperature abuse during their transport along the supply chain or during storage in household refrigerators, could assist in explaining the mismatch between clinical and food samples during outbreak investigations.					
35651000	9	8	theme	Camembert-based	1785:1799	arg1	substrates					1801:1810	in/on Camembert-based substrates	1779:1810	in/on Camembert-based substrates	1779:1810	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	4	9	theme	monocytogenes	682:694	arg1	strains					696:702	L. monocytogenes strains	679:702	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b)	679:750	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	9	theme	monocytogenes	682:694	arg1	ScottA					711:716	ScottA	711:716	ScottA	711:716	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	9	theme	monocytogenes	682:694	arg1	C5					704:705	C5	704:705	C5	704:705	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	12	10	dep	assist	2571:2576	arg1	Investigating					2348:2360	Investigating	2348:2360	Investigating inter-strain interactions during growth in/on	2348:2406	Investigating inter-strain interactions during growth in/on different substrates, which may have undergone temperature abuse during their transport along the supply chain or during storage in household refrigerators, could assist in explaining the mismatch between clinical and food samples during outbreak investigations.					
35651000	10	11	from	pronounced	2099:2108	arg1	broths					2125:2130	dairy-based broths	2113:2130	dairy-based broths	2113:2130	Under aerobic and hypoxic conditions, most of the observed interactions were more pronounced in dairy-based broths and were mitigated with the addition of agar.					
35651000	3	12	from	effect	501:506	arg1	interactions					568:579	inter-strain interactions	555:579	inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems	555:676	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
35651000	1	13	theme	ubiquitous	187:196	arg1	character					198:206	the ubiquitous character	183:206	the ubiquitous character of Listeria monocytogenes	183:232	Due to the ubiquitous character of Listeria monocytogenes multiple strains of the pathogen may end up co-existing in/on the same final products and could potentially cause infection during consumption.					
35651000	4	14	theme	dairy	1022:1026	arg1	broth					1028:1032	dairy broth	1022:1032	dairy broth supplemented with 0.6 and 1.4% agar	1022:1068	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	0	15	theme	Listeria	119:126	arg1	monocytogenes					128:140	Listeria monocytogenes	119:140	Listeria monocytogenes	119:140	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	7	16	theme	co-cultured	1508:1518	arg1	strains					1520:1526	the co-cultured strains	1504:1526	the co-cultured strains	1504:1526	The simultaneously presence of different strains of the pathogen in/on the same substrate did not affect neither the duration of the lag phase nor the growth rate of the co-cultured strains.					
35651000	8	17	dep	6.0	1699:1701	arg1	ca.					1695:1697	ca.	1695:1697	ca.	1695:1697	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	10	18	theme	agar	2172:2175	arg1	addition					2160:2167	the addition	2156:2167	the addition of agar	2156:2175	Under aerobic and hypoxic conditions, most of the observed interactions were more pronounced in dairy-based broths and were mitigated with the addition of agar.					
35651000	4	19	theme	Ringer	965:970	arg1	solution					972:979	2 ¼ Ringer solution	961:979	2 ¼ Ringer solution	961:979	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	1	20	theme	monocytogenes	220:232	arg1	character					198:206	the ubiquitous character	183:206	the ubiquitous character of Listeria monocytogenes	183:232	Due to the ubiquitous character of Listeria monocytogenes multiple strains of the pathogen may end up co-existing in/on the same final products and could potentially cause infection during consumption.					
35651000	4	21	theme	inoculated	870:879	arg1	media					1015:1019	inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media	870:1019	inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar)	870:1069	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	22	theme	single	1075:1080	arg1	ratio					1118:1122	1:1 strain ratio	1107:1122	1:1 strain ratio	1107:1122	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	22	theme	single	1075:1080	arg1	cultures					1097:1104	single and two-strain cultures	1075:1104	single and two-strain cultures (1:1 strain ratio)	1075:1123	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	0	23	theme	dairy	155:159	arg1	systems					167:173	different dairy model systems	145:173	different dairy model systems	145:173	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	8	24	theme	"	1671:1671	arg1	density					1684:1690	the "critical" population density	1658:1690	the "critical" population density of ca. 6.0 log CFU/mL	1658:1712	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	7	25	theme	growth	1489:1494	arg1	rate					1496:1499	the growth rate	1485:1499	the growth rate of the co-cultured strains	1485:1526	The simultaneously presence of different strains of the pathogen in/on the same substrate did not affect neither the duration of the lag phase nor the growth rate of the co-cultured strains.					
35651000	0	26	from	interactions	103:114	arg1	systems					167:173	different dairy model systems	145:173	different dairy model systems	145:173	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	4	27	theme	two-strain	1086:1095	arg1	ratio					1118:1122	1:1 strain ratio	1107:1122	1:1 strain ratio	1107:1122	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	27	theme	two-strain	1086:1095	arg1	cultures					1097:1104	single and two-strain cultures	1075:1104	single and two-strain cultures (1:1 strain ratio)	1075:1123	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	12	28	theme	household	2540:2548	arg1	refrigerators					2550:2562	household refrigerators	2540:2562	household refrigerators	2540:2562	Investigating inter-strain interactions during growth in/on different substrates, which may have undergone temperature abuse during their transport along the supply chain or during storage in household refrigerators, could assist in explaining the mismatch between clinical and food samples during outbreak investigations.					
35651000	3	29	theme	different	648:656	arg1	systems					670:676	different dairy model systems	648:676	different dairy model systems	648:676	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
35651000	4	30	dep	%	1063:1063	arg1	1.4					1060:1062	1.4	1060:1062	1.4	1060:1062	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	30	dep	%	1063:1063	arg1	0.6					1052:1054	0.6	1052:1054	0.6	1052:1054	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	12	31	from	storage	2529:2535	arg1	refrigerators					2550:2562	household refrigerators	2540:2562	household refrigerators	2540:2562	Investigating inter-strain interactions during growth in/on different substrates, which may have undergone temperature abuse during their transport along the supply chain or during storage in household refrigerators, could assist in explaining the mismatch between clinical and food samples during outbreak investigations.					
35651000	12	32	theme	supply	2506:2511	arg1	chain					2513:2517	the supply chain	2502:2517	the supply chain	2502:2517	Investigating inter-strain interactions during growth in/on different substrates, which may have undergone temperature abuse during their transport along the supply chain or during storage in household refrigerators, could assist in explaining the mismatch between clinical and food samples during outbreak investigations.					
35651000	0	33	theme	structure	54:62	arg1	effect					13:18	the effect	9:18	the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems	9:173	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	7	34	theme	lag	1471:1473	arg1	phase					1475:1479	the lag phase	1467:1479	the lag phase	1467:1479	The simultaneously presence of different strains of the pathogen in/on the same substrate did not affect neither the duration of the lag phase nor the growth rate of the co-cultured strains.					
35651000	0	35	from	effect	13:18	arg1	density					78:84	population density	67:84	population density	67:84	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	0	35	from	effect	13:18	arg1	interactions					103:114	inter-strain interactions	90:114	inter-strain interactions	90:114	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	0	36	theme	population	67:76	arg1	density					78:84	population density	67:84	population density	67:84	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	8	37	theme	log	1703:1705	arg1	CFU/mL					1707:1712	ca. 6.0 log CFU/mL	1695:1712	ca. 6.0 log CFU/mL	1695:1712	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	11	38	theme	microbial	2330:2338	arg1	counts					2340:2345	low microbial counts	2326:2345	low microbial counts	2326:2345	The elimination of oxygen resulted in a prolonged lag time, which lasted at least 5 days and no observed interactions by the end of storage, due to low microbial counts.					
35651000	10	39	theme	aerobic	2023:2029	arg1	conditions					2043:2052	aerobic and hypoxic conditions	2023:2052	aerobic and hypoxic conditions	2023:2052	Under aerobic and hypoxic conditions, most of the observed interactions were more pronounced in dairy-based broths and were mitigated with the addition of agar.					
35651000	6	40	theme	experimental	1232:1243	arg1	treatment					1245:1253	Every experimental treatment	1226:1253	Every experimental treatment	1226:1253	Every experimental treatment was tested with three biological replicates and two technical repeats (n = 3 × 2).					
35651000	4	41	from	enumeration	824:834	arg1	co-culture					854:863	co-culture	854:863	co-culture	854:863	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	2	42	theme	chain	462:466	arg1	stages					436:441	different stages	426:441	different stages of the food supply chain	426:466	Such multiple strain contamination may occur in different stages of the food supply chain.					
35651000	2	43	theme	food	450:453	arg1	chain					462:466	the food supply chain	446:466	the food supply chain	446:466	Such multiple strain contamination may occur in different stages of the food supply chain.					
35651000	4	44	theme	strain	1111:1116	arg1	ratio					1118:1122	1:1 strain ratio	1107:1122	1:1 strain ratio	1107:1122	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	44	theme	strain	1111:1116	arg1	cultures					1097:1104	single and two-strain cultures	1075:1104	single and two-strain cultures (1:1 strain ratio)	1075:1123	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	3	45	theme	present	473:479	arg1	study					481:485	The present study	469:485	The present study	469:485	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
35651000	9	46	dep	composition	1833:1843	arg1	the					1829:1831	the	1829:1831	the	1829:1831	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	11	47	theme	observed	2274:2281	arg1	interactions					2283:2294	no observed interactions	2271:2294	no observed interactions	2271:2294	The elimination of oxygen resulted in a prolonged lag time, which lasted at least 5 days and no observed interactions by the end of storage, due to low microbial counts.					
35651000	9	48	theme	final	1954:1958	arg1	level					1971:1975	the final population level	1950:1975	the final population level	1950:1975	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	2	49	theme	different	426:434	arg1	stages					436:441	different stages	426:441	different stages of the food supply chain	426:466	Such multiple strain contamination may occur in different stages of the food supply chain.					
35651000	0	50	theme	oxygen	23:28	arg1	availability					30:41	oxygen availability	23:41	oxygen availability	23:41	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	1	51	theme	final	305:309	arg1	products					311:318	in/on the same final products	290:318	in/on the same final products	290:318	Due to the ubiquitous character of Listeria monocytogenes multiple strains of the pathogen may end up co-existing in/on the same final products and could potentially cause infection during consumption.					
35651000	6	52	theme	technical	1307:1315	arg1	n = 3 × 2					1326:1334	n = 3 × 2	1326:1334	n = 3 × 2	1326:1334	Every experimental treatment was tested with three biological replicates and two technical repeats (n = 3 × 2).					
35651000	6	52	theme	technical	1307:1315	arg1	repeats					1317:1323	two technical repeats	1303:1323	two technical repeats (n = 3 × 2)	1303:1335	Every experimental treatment was tested with three biological replicates and two technical repeats (n = 3 × 2).					
35651000	3	53	theme	model	664:668	arg1	systems					670:676	different dairy model systems	648:676	different dairy model systems	648:676	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
35651000	12	54	theme	clinical	2613:2620	arg1	samples					2631:2637	clinical and food samples	2613:2637	clinical and food samples	2613:2637	Investigating inter-strain interactions during growth in/on different substrates, which may have undergone temperature abuse during their transport along the supply chain or during storage in household refrigerators, could assist in explaining the mismatch between clinical and food samples during outbreak investigations.					
35651000	9	55	theme	population	1960:1969	arg1	level					1971:1975	the final population level	1950:1975	the final population level	1950:1975	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	4	56	theme	structured	1004:1013	arg1	media					1015:1019	inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media	870:1019	inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar)	870:1069	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	8	57	with	related	1573:1579	arg1	reached/decrease					1607:1622	the final population reached/decrease	1586:1622	the final population reached/decrease during storage	1586:1637	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	11	58	theme	storage	2310:2316	arg1	end					2303:2305	the end	2299:2305	the end of storage	2299:2316	The elimination of oxygen resulted in a prolonged lag time, which lasted at least 5 days and no observed interactions by the end of storage, due to low microbial counts.					
35651000	1	59	theme	multiple	234:241	arg1	strains					243:249	Due to the ubiquitous character of Listeria monocytogenes multiple strains	176:249	Due to the ubiquitous character of Listeria monocytogenes multiple strains of the pathogen	176:265	Due to the ubiquitous character of Listeria monocytogenes multiple strains of the pathogen may end up co-existing in/on the same final products and could potentially cause infection during consumption.					
35651000	7	60	theme	pathogen	1394:1401	arg1	in/on					1403:1407	the pathogen in/on the same substrate	1390:1426	the pathogen in/on the same substrate	1390:1426	The simultaneously presence of different strains of the pathogen in/on the same substrate did not affect neither the duration of the lag phase nor the growth rate of the co-cultured strains.					
35651000	4	61	dep	strains	696:702	arg1	6179					724:727	6179	724:727	6179	724:727	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	61	dep	strains	696:702	arg1	ScottA					711:716	ScottA	711:716	ScottA	711:716	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	61	dep	strains	696:702	arg1	4b					719:720	4b	719:720	4b	719:720	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	61	dep	strains	696:702	arg1	PL25					740:743	PL25	740:743	PL25	740:743	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	61	dep	strains	696:702	arg1	strains					696:702	L. monocytogenes strains	679:702	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b)	679:750	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	61	dep	strains	696:702	arg1	C5					704:705	C5	704:705	C5	704:705	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	2	62	theme	multiple	383:390	arg1	contamination					399:411	Such multiple strain contamination	378:411	Such multiple strain contamination	378:411	Such multiple strain contamination may occur in different stages of the food supply chain.					
35651000	8	63	theme	inter-strain	1542:1553	arg1	related					1573:1579	related	1573:1579	related	1573:1579	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	8	63	theme	inter-strain	1542:1553	arg1	interactions					1555:1566	The observed inter-strain interactions	1529:1566	The observed inter-strain interactions	1529:1566	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	5	64	theme	Bacterial	1126:1134	arg1	growth					1136:1141	Bacterial growth	1126:1141	Bacterial growth	1126:1141	Bacterial growth was assessed during storage at 7 °C, under aerobic, hypoxic and anoxic conditions.					
35651000	9	65	theme	pronounced	1749:1758	arg1	Ricotta					1766:1772	more pronounced in/on Ricotta	1744:1772	more pronounced in/on Ricotta	1744:1772	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	9	65	theme	pronounced	1749:1758	arg1	phenomenon					1729:1738	The phenomenon	1725:1738	The phenomenon	1725:1738	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	4	66	dep	product	952:958	arg1	solution					972:979	2 ¼ Ringer solution	961:979	2 ¼ Ringer solution	961:979	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	3	67	theme	oxygen	511:516	arg1	availability					518:529	oxygen availability	511:529	oxygen availability	511:529	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
35651000	1	68	theme	pathogen	258:265	arg1	strains					243:249	Due to the ubiquitous character of Listeria monocytogenes multiple strains	176:249	Due to the ubiquitous character of Listeria monocytogenes multiple strains of the pathogen	176:265	Due to the ubiquitous character of Listeria monocytogenes multiple strains of the pathogen may end up co-existing in/on the same final products and could potentially cause infection during consumption.					
35651000	7	69	theme	different	1369:1377	arg1	strains					1379:1385	different strains	1369:1385	different strains of the pathogen in/on the same substrate	1369:1426	The simultaneously presence of different strains of the pathogen in/on the same substrate did not affect neither the duration of the lag phase nor the growth rate of the co-cultured strains.					
35651000	4	70	theme	strain	844:849	arg1	enumeration					824:834	selective enumeration	814:834	selective enumeration of each strain in co-culture	814:863	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	11	71	theme	lag	2228:2230	arg1	time					2232:2235	a prolonged lag time	2216:2235	a prolonged lag time	2216:2235	The elimination of oxygen resulted in a prolonged lag time, which lasted at least 5 days and no observed interactions by the end of storage, due to low microbial counts.					
35651000	3	72	theme	inter-strain	555:566	arg1	interactions					568:579	inter-strain interactions	555:579	inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems	555:676	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
35651000	9	73	theme	in/on	1779:1783	arg1	substrates					1801:1810	in/on Camembert-based substrates	1779:1810	in/on Camembert-based substrates	1779:1810	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	3	74	theme	structure	542:550	arg1	effect					501:506	the effect	497:506	the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems	497:676	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
35651000	8	75	theme	final	1590:1594	arg1	reached/decrease					1607:1622	the final population reached/decrease	1586:1622	the final population reached/decrease during storage	1586:1637	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	1	76	theme	Due	176:178	arg1	strains					243:249	Due to the ubiquitous character of Listeria monocytogenes multiple strains	176:249	Due to the ubiquitous character of Listeria monocytogenes multiple strains of the pathogen	176:265	Due to the ubiquitous character of Listeria monocytogenes multiple strains of the pathogen may end up co-existing in/on the same final products and could potentially cause infection during consumption.					
35651000	4	77	theme	selective	814:822	arg1	enumeration					824:834	selective enumeration	814:834	selective enumeration of each strain in co-culture	814:863	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	12	78	theme	temperature	2455:2465	arg1	abuse					2467:2471	temperature abuse	2455:2471	temperature abuse	2455:2471	Investigating inter-strain interactions during growth in/on different substrates, which may have undergone temperature abuse during their transport along the supply chain or during storage in household refrigerators, could assist in explaining the mismatch between clinical and food samples during outbreak investigations.					
35651000	3	79	theme	monocytogenes	587:599	arg1	interactions					568:579	inter-strain interactions	555:579	inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems	555:676	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
35651000	4	80	dep	agar	1065:1068	arg1	%					1063:1063	%	1063:1063	%	1063:1063	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	7	81	theme	strains	1520:1526	arg1	rate					1496:1499	the growth rate	1485:1499	the growth rate of the co-cultured strains	1485:1526	The simultaneously presence of different strains of the pathogen in/on the same substrate did not affect neither the duration of the lag phase nor the growth rate of the co-cultured strains.					
35651000	12	82	theme	food	2626:2629	arg1	samples					2631:2637	clinical and food samples	2613:2637	clinical and food samples	2613:2637	Investigating inter-strain interactions during growth in/on different substrates, which may have undergone temperature abuse during their transport along the supply chain or during storage in household refrigerators, could assist in explaining the mismatch between clinical and food samples during outbreak investigations.					
35651000	0	83	theme	monocytogenes	128:140	arg1	density					78:84	population density	67:84	population density	67:84	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	0	83	theme	monocytogenes	128:140	arg1	interactions					103:114	inter-strain interactions	90:114	inter-strain interactions	90:114	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	4	84	theme	log	890:892	arg1	CFU/mL					894:899	2.0-3.0 log CFU/mL	882:899	2.0-3.0 log CFU/mL	882:899	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	84	theme	log	890:892	arg1	cm2					907:909	cm2	907:909	cm2	907:909	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	84	theme	log	890:892	arg1	g					902:902	g	902:902	g	902:902	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	0	85	from	density	78:84	arg1	systems					167:173	different dairy model systems	145:173	different dairy model systems	145:173	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	10	86	theme	hypoxic	2035:2041	arg1	conditions					2043:2052	aerobic and hypoxic conditions	2023:2052	aerobic and hypoxic conditions	2023:2052	Under aerobic and hypoxic conditions, most of the observed interactions were more pronounced in dairy-based broths and were mitigated with the addition of agar.					
35651000	0	87	theme	different	145:153	arg1	systems					167:173	different dairy model systems	145:173	different dairy model systems	145:173	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	1	88	theme	Listeria	211:218	arg1	monocytogenes					220:232	Listeria monocytogenes	211:232	Listeria monocytogenes	211:232	Due to the ubiquitous character of Listeria monocytogenes multiple strains of the pathogen may end up co-existing in/on the same final products and could potentially cause infection during consumption.					
35651000	10	89	theme	observed	2067:2074	arg1	interactions					2076:2087	the observed interactions	2063:2087	the observed interactions	2063:2087	Under aerobic and hypoxic conditions, most of the observed interactions were more pronounced in dairy-based broths and were mitigated with the addition of agar.					
35651000	0	90	theme	model	161:165	arg1	systems					167:173	different dairy model systems	145:173	different dairy model systems	145:173	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	1	91	dep	end	271:273	arg1	up					275:276	up	275:276	up	275:276	Due to the ubiquitous character of Listeria monocytogenes multiple strains of the pathogen may end up co-existing in/on the same final products and could potentially cause infection during consumption.					
35651000	9	92	theme	available	1853:1861	arg1	nutrients					1863:1871	the available nutrients	1849:1871	the available nutrients	1849:1871	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	12	93	theme	inter-strain	2362:2373	arg1	interactions					2375:2386	inter-strain interactions	2362:2386	inter-strain interactions	2362:2386	Investigating inter-strain interactions during growth in/on different substrates, which may have undergone temperature abuse during their transport along the supply chain or during storage in household refrigerators, could assist in explaining the mismatch between clinical and food samples during outbreak investigations.					
35651000	8	94	theme	critical	1663:1670	arg1	density					1684:1690	the "critical" population density	1658:1690	the "critical" population density of ca. 6.0 log CFU/mL	1658:1712	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	7	95	theme	phase	1475:1479	arg1	duration					1455:1462	the duration	1451:1462	the duration of the lag phase	1451:1479	The simultaneously presence of different strains of the pathogen in/on the same substrate did not affect neither the duration of the lag phase nor the growth rate of the co-cultured strains.					
35651000	10	96	from	broths	2125:2130	arg1	pronounced					2099:2108	pronounced	2099:2108	pronounced	2099:2108	Under aerobic and hypoxic conditions, most of the observed interactions were more pronounced in dairy-based broths and were mitigated with the addition of agar.					
35651000	3	97	theme	population	624:633	arg1	levels					635:640	high population levels	619:640	high population levels	619:640	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
35651000	8	98	theme	population	1673:1682	arg1	density					1684:1690	the "critical" population density	1658:1690	the "critical" population density of ca. 6.0 log CFU/mL	1658:1712	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	11	99	theme	prolonged	2218:2226	arg1	time					2232:2235	a prolonged lag time	2216:2235	a prolonged lag time	2216:2235	The elimination of oxygen resulted in a prolonged lag time, which lasted at least 5 days and no observed interactions by the end of storage, due to low microbial counts.					
35651000	9	100	theme	substrate	1880:1888	arg1	nutrients					1863:1871	the available nutrients	1849:1871	the available nutrients	1849:1871	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	9	100	theme	substrate	1880:1888	arg1	composition					1833:1843	composition	1833:1843	composition	1833:1843	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	4	101	dep	inoculated	870:879	arg1	CFU/mL					894:899	2.0-3.0 log CFU/mL	882:899	2.0-3.0 log CFU/mL	882:899	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	101	dep	inoculated	870:879	arg1	product					952:958	1 dairy product	944:958	1 dairy product	944:958	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	101	dep	inoculated	870:879	arg1	cm2					907:909	cm2	907:909	cm2	907:909	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	101	dep	inoculated	870:879	arg1	g					902:902	g	902:902	g	902:902	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	11	102	theme	low	2326:2328	arg1	counts					2340:2345	low microbial counts	2326:2345	low microbial counts	2326:2345	The elimination of oxygen resulted in a prolonged lag time, which lasted at least 5 days and no observed interactions by the end of storage, due to low microbial counts.					
35651000	0	103	theme	inter-strain	90:101	arg1	interactions					103:114	inter-strain interactions	90:114	inter-strain interactions	90:114	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	10	104	theme	dairy-based	2113:2123	arg1	broths					2125:2130	dairy-based broths	2113:2130	dairy-based broths	2113:2130	Under aerobic and hypoxic conditions, most of the observed interactions were more pronounced in dairy-based broths and were mitigated with the addition of agar.					
35651000	5	105	theme	anoxic	1207:1212	arg1	conditions					1214:1223	aerobic, hypoxic and anoxic conditions	1186:1223	aerobic, hypoxic and anoxic conditions	1186:1223	Bacterial growth was assessed during storage at 7 °C, under aerobic, hypoxic and anoxic conditions.					
35651000	8	106	theme	CFU/mL	1707:1712	arg1	density					1684:1690	the "critical" population density	1658:1690	the "critical" population density of ca. 6.0 log CFU/mL	1658:1712	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	8	106	theme	CFU/mL	1707:1712	arg1	cm2					1720:1722	cm2	1720:1722	cm2	1720:1722	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	8	106	theme	CFU/mL	1707:1712	arg1	g					1715:1715	g	1715:1715	g	1715:1715	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	4	107	theme	1:1	1107:1109	arg1	ratio					1118:1122	1:1 strain ratio	1107:1122	1:1 strain ratio	1107:1122	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	107	theme	1:1	1107:1109	arg1	cultures					1097:1104	single and two-strain cultures	1075:1104	single and two-strain cultures (1:1 strain ratio)	1075:1123	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	4	108	dep	media	1015:1019	arg1	broth					1028:1032	dairy broth	1022:1032	dairy broth supplemented with 0.6 and 1.4% agar	1022:1068	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	2	109	theme	supply	455:460	arg1	chain					462:466	the food supply chain	446:466	the food supply chain	446:466	Such multiple strain contamination may occur in different stages of the food supply chain.					
35651000	5	110	theme	aerobic	1186:1192	arg1	conditions					1214:1223	aerobic, hypoxic and anoxic conditions	1186:1223	aerobic, hypoxic and anoxic conditions	1186:1223	Bacterial growth was assessed during storage at 7 °C, under aerobic, hypoxic and anoxic conditions.					
35651000	2	111	theme	strain	392:397	arg1	contamination					399:411	Such multiple strain contamination	378:411	Such multiple strain contamination	378:411	Such multiple strain contamination may occur in different stages of the food supply chain.					
35651000	11	112	theme	oxygen	2197:2202	arg1	elimination					2182:2192	The elimination	2178:2192	The elimination of oxygen	2178:2202	The elimination of oxygen resulted in a prolonged lag time, which lasted at least 5 days and no observed interactions by the end of storage, due to low microbial counts.					
35651000	4	113	theme	¼	963:963	arg1	solution					972:979	2 ¼ Ringer solution	961:979	2 ¼ Ringer solution	961:979	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	5	114	theme	hypoxic	1195:1201	arg1	conditions					1214:1223	aerobic, hypoxic and anoxic conditions	1186:1223	aerobic, hypoxic and anoxic conditions	1186:1223	Bacterial growth was assessed during storage at 7 °C, under aerobic, hypoxic and anoxic conditions.					
35651000	0	115	theme	availability	30:41	arg1	effect					13:18	the effect	9:18	the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems	9:173	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	1	116	theme	same	300:303	arg1	products					311:318	in/on the same final products	290:318	in/on the same final products	290:318	Due to the ubiquitous character of Listeria monocytogenes multiple strains of the pathogen may end up co-existing in/on the same final products and could potentially cause infection during consumption.					
35651000	4	117	dep	antibiotics	792:802	arg1	enabling					805:812	enabling	805:812	enabling selective enumeration of each strain in co-culture	805:863	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	3	118	theme	dairy	658:662	arg1	systems					670:676	different dairy model systems	648:676	different dairy model systems	648:676	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
35651000	4	119	theme	different	782:790	arg1	antibiotics					792:802	different antibiotics	782:802	different antibiotics (enabling selective enumeration of each strain in co-culture)	782:864	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	0	120	theme	matrix	47:52	arg1	structure					54:62	matrix structure	47:62	matrix structure	47:62	Studying the effect of oxygen availability and matrix structure on population density and inter-strain interactions of Listeria monocytogenes in different dairy model systems.					
35651000	9	121	from	difference	1936:1945	arg1	level					1971:1975	the final population level	1950:1975	the final population level	1950:1975	The phenomenon was more pronounced in/on Ricotta than in/on Camembert-based substrates, indicating that the composition and the available nutrients of the substrate may affect the interactions that expressed as difference in the final population level between singly and co-cultured strains.					
35651000	4	122	theme	dairy	946:950	arg1	product					952:958	1 dairy product	944:958	1 dairy product	944:958	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	8	123	theme	observed	1533:1540	arg1	related					1573:1579	related	1573:1579	related	1573:1579	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	8	123	theme	observed	1533:1540	arg1	interactions					1555:1566	The observed inter-strain interactions	1529:1566	The observed inter-strain interactions	1529:1566	The observed inter-strain interactions were related with the final population reached/decrease during storage and occurred after the "critical" population density of ca. 6.0 log CFU/mL, g or cm2.					
35651000	7	124	theme	same	1413:1416	arg1	substrate					1418:1426	the same substrate	1409:1426	the pathogen in/on the same substrate	1390:1426	The simultaneously presence of different strains of the pathogen in/on the same substrate did not affect neither the duration of the lag phase nor the growth rate of the co-cultured strains.					
35651000	3	125	theme	high	619:622	arg1	levels					635:640	high population levels	619:640	high population levels	619:640	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
35651000	4	126	theme	dairy-based	992:1002	arg1	media					1015:1019	inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media	870:1019	inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar)	870:1069	L. monocytogenes strains C5 and ScottA (4b), 6179 (1/2a) and PL25 (1/2b) were selected as resistant to different antibiotics (enabling selective enumeration of each strain in co-culture) and inoculated (2.0-3.0 log CFU/mL, g or cm2) in Ricotta and Camembert broth (1 dairy product: 2 ¼ Ringer solution) and in/on dairy-based structured media (dairy broth supplemented with 0.6 and 1.4% agar), in single and two-strain cultures (1:1 strain ratio).					
35651000	2	127	theme	Such	378:381	arg1	contamination					399:411	Such multiple strain contamination	378:411	Such multiple strain contamination	378:411	Such multiple strain contamination may occur in different stages of the food supply chain.					
35651000	7	128	theme	in/on	1403:1407	arg1	strains					1379:1385	different strains	1369:1385	different strains of the pathogen in/on the same substrate	1369:1426	The simultaneously presence of different strains of the pathogen in/on the same substrate did not affect neither the duration of the lag phase nor the growth rate of the co-cultured strains.					
35651000	3	129	theme	matrix	535:540	arg1	structure					542:550	matrix structure	535:550	matrix structure	535:550	The present study evaluated the effect of oxygen availability and matrix structure on inter-strain interactions of L. monocytogenes that may occur at high population levels in/on different dairy model systems.					
37038307	7	0	from	components	1486:1495	arg1	sector					1509:1514	the food sector	1500:1514	the food sector	1500:1514	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	8	1	theme	modified	1607:1614	arg1	IDF					1641:1643	IDF	1641:1643	IDF	1641:1643	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	8	1	theme	modified	1607:1614	arg1	fiber					1634:1638	modified insoluble dietary fiber	1607:1638	modified insoluble dietary fiber (IDF) with different purities in okara	1607:1677	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	6	2	theme	at	1273:1274	arg1	pH					1276:1277	10.94-19.37 mg/g at pH 2	1256:1279	14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2	1230:1279	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	7	3	theme	high-purity	1412:1422	arg1	T4					1445:1446	T4	1445:1446	T4	1445:1446	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	7	3	theme	high-purity	1412:1422	arg1	T3					1438:1439	T3	1438:1439	T3	1438:1439	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	7	3	theme	high-purity	1412:1422	arg1	modified-IDFs					1424:1436	the high-purity modified-IDFs T3 and T4	1408:1446	the high-purity modified-IDFs T3 and T4	1408:1446	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	8	4	theme	dietary	1626:1632	arg1	IDF					1641:1643	IDF	1641:1643	IDF	1641:1643	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	8	4	theme	dietary	1626:1632	arg1	fiber					1634:1638	modified insoluble dietary fiber	1607:1638	modified insoluble dietary fiber (IDF) with different purities in okara	1607:1677	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	6	5	from	pH	1250:1251	arg1	14.54-33.56 mg/g					1230:1245	14.54-33.56 mg/g	1230:1245	14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2	1230:1279	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	4	6	theme	cell	634:637	arg1	wall					639:642	the cell wall	630:642	the cell wall of okara	630:651	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	9	7	dep	in	1907:1908	arg1	vivo					1910:1913	vivo	1910:1913	vivo	1910:1913	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	2	8	from	okara	324:328	arg1	IDFs					314:317	IDFs	314:317	IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L	314:400	This study was intended to investigate the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L.					
37038307	2	8	from	okara	324:328	arg1	effect					273:278	the effect	269:278	the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L	269:400	This study was intended to investigate the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L.					
37038307	6	9	theme	improved	1152:1159	arg1	capacities					1172:1181	improved adsorption capacities	1152:1181	improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity	1152:1329	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	9	10	theme	illnesses	1869:1877	arg1	treatment					1851:1859	the treatment	1847:1859	the treatment of some illnesses due to its well-established in vivo function	1847:1922	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	2	11	theme	extraction	283:292	arg1	effect					273:278	the effect	269:278	the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L	269:400	This study was intended to investigate the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L.					
37038307	5	12	theme	relative	854:861	arg1	crystallinity					863:875	relative crystallinity	854:875	relative crystallinity	854:875	In accordance with the X-ray diffraction and Fourier-transform infrared spectrum investigations, relative crystallinity decreased and certain cellulose components were reallocated and changed into soluble dietary fiber.					
37038307	8	13	from	purities	1661:1668	arg1	okara					1673:1677	okara	1673:1677	okara	1673:1677	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	6	14	theme	better	1299:1304	arg1	capacity					1322:1329	better cation-exchange capacity	1299:1329	better cation-exchange capacity	1299:1329	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	1	15	theme	dietary	209:215	arg1	IDF					224:226	IDF	224:226	IDF	224:226	The endogenous components of okara can affect the extraction and modification of insoluble dietary fiber (IDF).					
37038307	1	15	theme	dietary	209:215	arg1	fiber					217:221	insoluble dietary fiber	199:221	insoluble dietary fiber (IDF)	199:227	The endogenous components of okara can affect the extraction and modification of insoluble dietary fiber (IDF).					
37038307	9	16	theme	well-established	1890:1905	arg1	function					1915:1922	its well-established in vivo function	1886:1922	its well-established in vivo function	1886:1922	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	6	17	theme	cholate	1287:1293	arg1	capacities					1172:1181	improved adsorption capacities	1152:1181	improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity	1152:1329	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	5	18	theme	infrared	820:827	arg1	spectrum					829:836	Fourier-transform infrared spectrum	802:836	Fourier-transform infrared spectrum	802:836	In accordance with the X-ray diffraction and Fourier-transform infrared spectrum investigations, relative crystallinity decreased and certain cellulose components were reallocated and changed into soluble dietary fiber.					
37038307	9	19	theme	high-purity	1693:1703	arg1	IDF					1714:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	9	19	theme	high-purity	1693:1703	arg1	material					1760:1767	a functional fiber raw material	1737:1767	a functional fiber raw material in the manufacture of functional foods	1737:1806	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	4	20	theme	specific	681:688	arg1	area					698:701	specific surface area	681:701	specific surface area of IDF and the formation of honeycomb-like structure	681:754	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	8	21	dep	APPLICATION	1527:1537	arg1	advances					1551:1558	advances	1551:1558	advances	1551:1558	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	6	22	theme	glucose	1186:1192	arg1	capacities					1172:1181	improved adsorption capacities	1152:1181	improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity	1152:1329	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	7	23	dep	modified-IDFs	1424:1436	arg1	T4					1445:1446	T4	1445:1446	T4	1445:1446	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	7	23	dep	modified-IDFs	1424:1436	arg1	T3					1438:1439	T3	1438:1439	T3	1438:1439	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	7	23	dep	modified-IDFs	1424:1436	arg1	modified-IDFs					1424:1436	the high-purity modified-IDFs T3 and T4	1408:1446	the high-purity modified-IDFs T3 and T4	1408:1446	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	6	24	theme	water-holding	1020:1032	arg1	10.73-11.59 g/g					1044:1058	10.73-11.59 g/g	1044:1058	10.73-11.59 g/g	1044:1058	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	6	24	theme	water-holding	1020:1032	arg1	capacity					1034:1041	improved water-holding capacity	1011:1041	improved water-holding capacity (10.73-11.59 g/g)	1011:1059	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	6	25	theme	maximum	1195:1201	arg1	39.60 mg/g					1204:1213	maximum: 39.60 mg/g	1195:1213	maximum: 39.60 mg/g	1195:1213	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	6	25	theme	maximum	1195:1201	arg1	glucose					1186:1192	glucose	1186:1192	glucose (maximum: 39.60 mg/g)	1186:1214	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	0	26	theme	insoluble	58:66	arg1	fiber					76:80	insoluble dietary fiber	58:80	insoluble dietary fiber	58:80	Structural, physicochemical, and functional properties of insoluble dietary fiber derived from okara by Viscozyme® L.					
37038307	5	27	theme	X-ray	780:784	arg1	diffraction					786:796	X-ray diffraction	780:796	X-ray diffraction	780:796	In accordance with the X-ray diffraction and Fourier-transform infrared spectrum investigations, relative crystallinity decreased and certain cellulose components were reallocated and changed into soluble dietary fiber.					
37038307	9	28	from	material	1760:1767	arg1	manufacture					1776:1786	the manufacture	1772:1786	the manufacture of functional foods	1772:1806	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	0	29	theme	fiber	76:80	arg1	properties					44:53	Structural, physicochemical, and functional properties	0:53	Structural, physicochemical, and functional properties of insoluble dietary fiber	0:80	Structural, physicochemical, and functional properties of insoluble dietary fiber derived from okara by Viscozyme® L.					
37038307	5	30	theme	certain	891:897	arg1	components					909:918	certain cellulose components	891:918	certain cellulose components	891:918	In accordance with the X-ray diffraction and Fourier-transform infrared spectrum investigations, relative crystallinity decreased and certain cellulose components were reallocated and changed into soluble dietary fiber.					
37038307	6	31	theme	modified	991:998	arg1	IDF					1000:1002	the modified IDF	987:1002	the modified IDF	987:1002	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	9	32	theme	functional	1739:1748	arg1	IDF					1714:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	9	32	theme	functional	1739:1748	arg1	material					1760:1767	a functional fiber raw material	1737:1767	a functional fiber raw material in the manufacture of functional foods	1737:1806	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	1	33	theme	endogenous	122:131	arg1	components					133:142	The endogenous components	118:142	The endogenous components of okara	118:151	The endogenous components of okara can affect the extraction and modification of insoluble dietary fiber (IDF).					
37038307	9	34	theme	raw	1756:1758	arg1	IDF					1714:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	9	34	theme	raw	1756:1758	arg1	material					1760:1767	a functional fiber raw material	1737:1767	a functional fiber raw material in the manufacture of functional foods	1737:1806	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	2	35	theme	composite	372:380	arg1	Viscozyme®					389:398	composite enzyme Viscozyme®	372:398	composite enzyme Viscozyme® L	372:400	This study was intended to investigate the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L.					
37038307	4	36	theme	deproteinizing	563:576	arg1	purification					535:546	the purification	531:546	the purification of degreasing, deproteinizing and destarching	531:592	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	0	37	theme	Structural	0:9	arg1	properties					44:53	Structural, physicochemical, and functional properties	0:53	Structural, physicochemical, and functional properties of insoluble dietary fiber	0:80	Structural, physicochemical, and functional properties of insoluble dietary fiber derived from okara by Viscozyme® L.					
37038307	6	38	theme	oil-holding	1062:1072	arg1	capacity					1074:1081	oil-holding capacity	1062:1081	oil-holding capacity (5.37-6.60 g/g)	1062:1097	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	6	38	theme	oil-holding	1062:1072	arg1	5.37-6.60 g/g					1084:1096	5.37-6.60 g/g	1084:1096	5.37-6.60 g/g	1084:1096	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	8	39	theme	PRACTICAL	1517:1525	arg1	APPLICATION					1527:1537	PRACTICAL APPLICATION	1517:1537	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.	1517:1678	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	4	40	theme	IDF	706:708	arg1	area					698:701	specific surface area	681:701	specific surface area of IDF and the formation of honeycomb-like structure	681:754	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	3	41	theme	functional	436:445	arg1	characteristics					463:477	the physicochemical, functional, and structural characteristics	415:477	the physicochemical, functional, and structural characteristics of IDFs	415:485	After that, the physicochemical, functional, and structural characteristics of IDFs were analyzed.					
37038307	0	42	theme	physicochemical	12:26	arg1	properties					44:53	Structural, physicochemical, and functional properties	0:53	Structural, physicochemical, and functional properties of insoluble dietary fiber	0:80	Structural, physicochemical, and functional properties of insoluble dietary fiber derived from okara by Viscozyme® L.					
37038307	5	43	theme	soluble	954:960	arg1	fiber					970:974	soluble dietary fiber	954:974	soluble dietary fiber	954:974	In accordance with the X-ray diffraction and Fourier-transform infrared spectrum investigations, relative crystallinity decreased and certain cellulose components were reallocated and changed into soluble dietary fiber.					
37038307	9	44	theme	functional	1791:1800	arg1	foods					1802:1806	functional foods	1791:1806	functional foods	1791:1806	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	2	45	dep	Viscozyme®	389:398	arg1	L					400:400	L	400:400	composite enzyme Viscozyme® L	372:400	This study was intended to investigate the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L.					
37038307	3	46	theme	physicochemical	419:433	arg1	characteristics					463:477	the physicochemical, functional, and structural characteristics	415:477	the physicochemical, functional, and structural characteristics of IDFs	415:485	After that, the physicochemical, functional, and structural characteristics of IDFs were analyzed.					
37038307	4	47	theme	structure	746:754	arg1	IDF					706:708	IDF	706:708	IDF	706:708	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	4	47	theme	structure	746:754	arg1	formation					718:726	the formation	714:726	the formation of honeycomb-like structure	714:754	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	8	48	theme	fiber	1634:1638	arg1	mechanism					1594:1602	the internal mechanism	1581:1602	the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara	1581:1677	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	1	49	theme	fiber	217:221	arg1	extraction					168:177	extraction	168:177	extraction	168:177	The endogenous components of okara can affect the extraction and modification of insoluble dietary fiber (IDF).					
37038307	1	49	theme	fiber	217:221	arg1	modification					183:194	modification	183:194	modification	183:194	The endogenous components of okara can affect the extraction and modification of insoluble dietary fiber (IDF).					
37038307	7	50	theme	functional	1469:1478	arg1	components					1486:1495	functional fiber components	1469:1495	functional fiber components in the food sector	1469:1514	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	4	51	theme	destarching	582:592	arg1	purification					535:546	the purification	531:546	the purification of degreasing, deproteinizing and destarching	531:592	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	6	52	from	pH	1276:1277	arg1	10.94-19.37 mg/g					1256:1271	10.94-19.37 mg/g at pH 2	1256:1279	14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2	1230:1279	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	6	52	from	pH	1276:1277	arg1	at					1273:1274	10.94-19.37 mg/g at pH 2	1256:1279	14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2	1230:1279	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	6	53	dep	14.54-33.56 mg/g	1230:1245	arg1	pH					1276:1277	10.94-19.37 mg/g at pH 2	1256:1279	14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2	1230:1279	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	8	54	theme	internal	1585:1592	arg1	mechanism					1594:1602	the internal mechanism	1581:1602	the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara	1581:1677	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	3	55	theme	IDFs	482:485	arg1	characteristics					463:477	the physicochemical, functional, and structural characteristics	415:477	the physicochemical, functional, and structural characteristics of IDFs	415:485	After that, the physicochemical, functional, and structural characteristics of IDFs were analyzed.					
37038307	4	56	theme	degreasing	551:560	arg1	purification					535:546	the purification	531:546	the purification of degreasing, deproteinizing and destarching	531:592	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	6	57	theme	10.94-19.37 mg/g	1256:1271	arg1	pH					1276:1277	10.94-19.37 mg/g at pH 2	1256:1279	14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2	1230:1279	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	7	58	dep	IDFs	1389:1392	arg1	T4					1445:1446	T4	1445:1446	T4	1445:1446	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	7	58	dep	IDFs	1389:1392	arg1	T3					1438:1439	T3	1438:1439	T3	1438:1439	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	7	58	dep	IDFs	1389:1392	arg1	modified-IDFs					1424:1436	the high-purity modified-IDFs T3 and T4	1408:1446	the high-purity modified-IDFs T3 and T4	1408:1446	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	8	59	theme	insoluble	1616:1624	arg1	IDF					1641:1643	IDF	1641:1643	IDF	1641:1643	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	8	59	theme	insoluble	1616:1624	arg1	fiber					1634:1638	modified insoluble dietary fiber	1607:1638	modified insoluble dietary fiber (IDF) with different purities in okara	1607:1677	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	5	60	theme	spectrum	829:836	arg1	investigations					838:851	the X-ray diffraction and Fourier-transform infrared spectrum investigations	776:851	the X-ray diffraction and Fourier-transform infrared spectrum investigations	776:851	In accordance with the X-ray diffraction and Fourier-transform infrared spectrum investigations, relative crystallinity decreased and certain cellulose components were reallocated and changed into soluble dietary fiber.					
37038307	6	61	theme	adsorption	1161:1170	arg1	capacities					1172:1181	improved adsorption capacities	1152:1181	improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity	1152:1329	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	6	62	theme	cation-exchange	1306:1320	arg1	capacity					1322:1329	better cation-exchange capacity	1299:1329	better cation-exchange capacity	1299:1329	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	5	63	theme	diffraction	786:796	arg1	investigations					838:851	the X-ray diffraction and Fourier-transform infrared spectrum investigations	776:851	the X-ray diffraction and Fourier-transform infrared spectrum investigations	776:851	In accordance with the X-ray diffraction and Fourier-transform infrared spectrum investigations, relative crystallinity decreased and certain cellulose components were reallocated and changed into soluble dietary fiber.					
37038307	7	64	theme	acquired	1380:1387	arg1	IDFs					1389:1392	the acquired IDFs	1376:1392	the acquired IDFs	1376:1392	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	5	65	theme	Fourier-transform	802:818	arg1	spectrum					829:836	Fourier-transform infrared spectrum	802:836	Fourier-transform infrared spectrum	802:836	In accordance with the X-ray diffraction and Fourier-transform infrared spectrum investigations, relative crystallinity decreased and certain cellulose components were reallocated and changed into soluble dietary fiber.					
37038307	9	66	theme	obtained	1684:1691	arg1	IDF					1714:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	9	66	theme	obtained	1684:1691	arg1	material					1760:1767	a functional fiber raw material	1737:1767	a functional fiber raw material in the manufacture of functional foods	1737:1806	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	6	67	theme	cholesterol	1217:1227	arg1	capacities					1172:1181	improved adsorption capacities	1152:1181	improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity	1152:1329	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	9	68	theme	in	1907:1908	arg1	function					1915:1922	its well-established in vivo function	1886:1922	its well-established in vivo function	1886:1922	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	5	69	theme	cellulose	899:907	arg1	components					909:918	certain cellulose components	891:918	certain cellulose components	891:918	In accordance with the X-ray diffraction and Fourier-transform infrared spectrum investigations, relative crystallinity decreased and certain cellulose components were reallocated and changed into soluble dietary fiber.					
37038307	9	70	theme	modified	1705:1712	arg1	IDF					1714:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	9	70	theme	modified	1705:1712	arg1	material					1760:1767	a functional fiber raw material	1737:1767	a functional fiber raw material in the manufacture of functional foods	1737:1806	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	0	71	theme	dietary	68:74	arg1	fiber					76:80	insoluble dietary fiber	58:80	insoluble dietary fiber	58:80	Structural, physicochemical, and functional properties of insoluble dietary fiber derived from okara by Viscozyme® L.					
37038307	6	72	theme	improved	1011:1018	arg1	10.73-11.59 g/g					1044:1058	10.73-11.59 g/g	1044:1058	10.73-11.59 g/g	1044:1058	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	6	72	theme	improved	1011:1018	arg1	capacity					1034:1041	improved water-holding capacity	1011:1041	improved water-holding capacity (10.73-11.59 g/g)	1011:1059	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	4	73	theme	okara	647:651	arg1	wall					639:642	the cell wall	630:642	the cell wall of okara	630:651	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	1	74	theme	okara	147:151	arg1	components					133:142	The endogenous components	118:142	The endogenous components of okara	118:151	The endogenous components of okara can affect the extraction and modification of insoluble dietary fiber (IDF).					
37038307	2	75	from	effect	273:278	arg1	okara					324:328	okara	324:328	okara affected by the endogenous components and composite enzyme Viscozyme® L	324:400	This study was intended to investigate the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L.					
37038307	6	76	dep	cholesterol	1217:1227	arg1	14.54-33.56 mg/g					1230:1245	14.54-33.56 mg/g	1230:1245	14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2	1230:1279	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	5	77	theme	dietary	962:968	arg1	fiber					970:974	soluble dietary fiber	954:974	soluble dietary fiber	954:974	In accordance with the X-ray diffraction and Fourier-transform infrared spectrum investigations, relative crystallinity decreased and certain cellulose components were reallocated and changed into soluble dietary fiber.					
37038307	9	78	theme	fiber	1750:1754	arg1	IDF					1714:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	9	78	theme	fiber	1750:1754	arg1	material					1760:1767	a functional fiber raw material	1737:1767	a functional fiber raw material in the manufacture of functional foods	1737:1806	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	3	79	theme	structural	452:461	arg1	characteristics					463:477	the physicochemical, functional, and structural characteristics	415:477	the physicochemical, functional, and structural characteristics of IDFs	415:485	After that, the physicochemical, functional, and structural characteristics of IDFs were analyzed.					
37038307	0	80	theme	Viscozyme®	104:113	arg1	L					115:115	Viscozyme® L	104:115	Viscozyme® L	104:115	Structural, physicochemical, and functional properties of insoluble dietary fiber derived from okara by Viscozyme® L.					
37038307	4	81	theme	formation	718:726	arg1	area					698:701	specific surface area	681:701	specific surface area of IDF and the formation of honeycomb-like structure	681:754	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	4	82	theme	surface	690:696	arg1	area					698:701	specific surface area	681:701	specific surface area of IDF and the formation of honeycomb-like structure	681:754	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	2	83	theme	endogenous	346:355	arg1	components					357:366	the endogenous components	342:366	the endogenous components	342:366	This study was intended to investigate the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L.					
37038307	9	84	used	utilized	1825:1832	arg2	material					1760:1767	a functional fiber raw material	1737:1767	a functional fiber raw material in the manufacture of functional foods	1737:1806	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	9	84	used	utilized	1825:1832	arg2	IDF					1714:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF	1680:1716	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	0	85	theme	functional	33:42	arg1	properties					44:53	Structural, physicochemical, and functional properties	0:53	Structural, physicochemical, and functional properties of insoluble dietary fiber	0:80	Structural, physicochemical, and functional properties of insoluble dietary fiber derived from okara by Viscozyme® L.					
37038307	1	86	theme	insoluble	199:207	arg1	IDF					224:226	IDF	224:226	IDF	224:226	The endogenous components of okara can affect the extraction and modification of insoluble dietary fiber (IDF).					
37038307	1	86	theme	insoluble	199:207	arg1	fiber					217:221	insoluble dietary fiber	199:221	insoluble dietary fiber (IDF)	199:227	The endogenous components of okara can affect the extraction and modification of insoluble dietary fiber (IDF).					
37038307	9	87	theme	foods	1802:1806	arg1	manufacture					1776:1786	the manufacture	1772:1786	the manufacture of functional foods	1772:1806	The obtained high-purity modified IDF can be employed as a functional fiber raw material in the manufacture of functional foods, and can also be utilized to assist in the treatment of some illnesses due to its well-established in vivo function.					
37038307	8	88	with	fiber	1634:1638	arg1	purities					1661:1668	different purities	1651:1668	different purities in okara	1651:1677	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	2	89	theme	enzyme	382:387	arg1	Viscozyme®					389:398	composite enzyme Viscozyme®	372:398	composite enzyme Viscozyme® L	372:400	This study was intended to investigate the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L.					
37038307	7	90	theme	food	1504:1507	arg1	sector					1509:1514	the food sector	1500:1514	the food sector	1500:1514	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	2	91	theme	IDFs	314:317	arg1	effect					273:278	the effect	269:278	the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L	269:400	This study was intended to investigate the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L.					
37038307	4	92	theme	honeycomb-like	731:744	arg1	structure					746:754	honeycomb-like structure	731:754	honeycomb-like structure	731:754	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	2	93	theme	purification	298:309	arg1	effect					273:278	the effect	269:278	the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L	269:400	This study was intended to investigate the effect of extraction and purification of IDFs from okara affected by the endogenous components and composite enzyme Viscozyme® L.					
37038307	4	94	from	increase	669:676	arg1	area					698:701	specific surface area	681:701	specific surface area of IDF and the formation of honeycomb-like structure	681:754	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	7	95	theme	fiber	1480:1484	arg1	components					1486:1495	functional fiber components	1469:1495	functional fiber components in the food sector	1469:1514	The outcomes demonstrated the potential for the acquired IDFs, particularly the high-purity modified-IDFs T3 and T4 , to be exploited as functional fiber components in the food sector.					
37038307	6	96	theme	swelling	1104:1111	arg1	8.99-12.37 mL/g					1123:1137	8.99-12.37 mL/g	1123:1137	8.99-12.37 mL/g	1123:1137	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	6	96	theme	swelling	1104:1111	arg1	capacity					1113:1120	swelling capacity	1104:1120	swelling capacity (8.99-12.37 mL/g)	1104:1138	Moreover, the modified IDF showed improved water-holding capacity (10.73-11.59 g/g), oil-holding capacity (5.37-6.60 g/g), and swelling capacity (8.99-12.37 mL/g), leading to improved adsorption capacities of glucose (maximum: 39.60 mg/g), cholesterol (14.54-33.56 mg/g at pH 7; 10.94-19.37 mg/g at pH 2), and cholate and better cation-exchange capacity.					
37038307	8	97	theme	mechanism	1594:1602	arg1	understanding					1564:1576	the understanding	1560:1576	the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara	1560:1677	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
37038307	4	98	theme	Viscozyme®	595:604	arg1	L					606:606	Viscozyme® L	595:606	Viscozyme® L	595:606	The results showed that with the purification of degreasing, deproteinizing and destarching, Viscozyme® L can accurately act on the cell wall of okara, leading to the increase in specific surface area of IDF and the formation of honeycomb-like structure.					
37038307	8	99	theme	different	1651:1659	arg1	purities					1661:1668	different purities	1651:1668	different purities in okara	1651:1677	PRACTICAL APPLICATION: This study advances the understanding of the internal mechanism of modified insoluble dietary fiber (IDF) with different purities in okara.					
36152593	3	0	theme	combined	361:368	arg1	pretreatment					370:381	A combined pretreatment	359:381	A combined pretreatment of NaOH and methyltrioctylammonium chloride	359:425	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	7	1	theme	purification	988:999	arg1	fold					1001:1004	purification fold	988:1004	purification fold	988:1004	The purification of EG by using DEAE column yielded specific activity and purification fold of 365.866 IU mg-1 and 4.264, respectively.					
36152593	10	2	theme	min-1	1393:1397	arg1	Vmax					1367:1370	Vmax	1367:1370	Vmax	1367:1370	The purified EG exhibited Vmax and Km of 294 U mL-1 min-1 and 36 µM, respectively, in the presence of 60 mM glucose.					
36152593	10	2	theme	min-1	1393:1397	arg1	Km					1376:1377	Km	1376:1377	Km	1376:1377	The purified EG exhibited Vmax and Km of 294 U mL-1 min-1 and 36 µM, respectively, in the presence of 60 mM glucose.					
36152593	7	3	theme	specific	966:973	arg1	activity					975:982	specific activity	966:982	specific activity	966:982	The purification of EG by using DEAE column yielded specific activity and purification fold of 365.866 IU mg-1 and 4.264, respectively.					
36152593	3	4	theme	chloride	418:425	arg1	pretreatment					370:381	A combined pretreatment	359:381	A combined pretreatment of NaOH and methyltrioctylammonium chloride	359:425	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	0	5	from	Characterization	0:15	arg1	sedimentimangrovi					93:109	sedimentimangrovi	93:109	sedimentimangrovi	93:109	Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25.					
36152593	10	6	theme	294 U mL-1	1382:1391	arg1	min-1					1393:1397	294 U mL-1 min-1	1382:1397	294 U mL-1 min-1	1382:1397	The purified EG exhibited Vmax and Km of 294 U mL-1 min-1 and 36 µM, respectively, in the presence of 60 mM glucose.					
36152593	10	7	theme	purified	1345:1352	arg1	EG					1354:1355	The purified EG	1341:1355	The purified EG	1341:1355	The purified EG exhibited Vmax and Km of 294 U mL-1 min-1 and 36 µM, respectively, in the presence of 60 mM glucose.					
36152593	2	8	theme	Neobacillus	323:333	arg1	UE25					353:356	Neobacillus sedimentimangrovi UE25	323:356	Neobacillus sedimentimangrovi UE25	323:356	This study was designed to characterize the cellulase obtained from a thermophilic bacterium, Neobacillus sedimentimangrovi UE25.					
36152593	2	8	theme	Neobacillus	323:333	arg1	bacterium					312:320	a thermophilic bacterium	297:320	a thermophilic bacterium	297:320	This study was designed to characterize the cellulase obtained from a thermophilic bacterium, Neobacillus sedimentimangrovi UE25.					
36152593	2	9	theme	sedimentimangrovi	335:351	arg1	UE25					353:356	Neobacillus sedimentimangrovi UE25	323:356	Neobacillus sedimentimangrovi UE25	323:356	This study was designed to characterize the cellulase obtained from a thermophilic bacterium, Neobacillus sedimentimangrovi UE25.					
36152593	2	9	theme	sedimentimangrovi	335:351	arg1	bacterium					312:320	a thermophilic bacterium	297:320	a thermophilic bacterium	297:320	This study was designed to characterize the cellulase obtained from a thermophilic bacterium, Neobacillus sedimentimangrovi UE25.					
36152593	4	10	theme	composite	645:653	arg1	design					655:660	central composite design	637:660	central composite design which has not been reported earlier for this purpose	637:713	The thermostable cellulase thus obtained was characterized by adopting central composite design which has not been reported earlier for this purpose.					
36152593	11	11	theme	industrial	1522:1531	arg1	sector					1533:1538	industrial sector	1522:1538	industrial sector	1522:1538	This novel thermostable cellulase can finds its applications in industrial sector.					
36152593	11	12	theme	novel	1463:1467	arg1	cellulase					1482:1490	This novel thermostable cellulase	1458:1490	This novel thermostable cellulase	1458:1490	This novel thermostable cellulase can finds its applications in industrial sector.					
36152593	4	13	theme	central	637:643	arg1	design					655:660	central composite design	637:660	central composite design which has not been reported earlier for this purpose	637:713	The thermostable cellulase thus obtained was characterized by adopting central composite design which has not been reported earlier for this purpose.					
36152593	6	14	from	80 ℃	908:911	arg1	stable					887:892	stable	887:892	stable	887:892	Endoglucanase (EG) was found to be stable for 30 min at 80 ℃.					
36152593	8	15	theme	purified	1054:1061	arg1	EG					1063:1064	The purified EG	1050:1064	The purified EG	1050:1064	The purified EG had a molecular weight of ∼45 kDa.					
36152593	9	16	theme	228.57	1165:1170	arg1	IU					1172:1173	IU	1172:1173	IU	1172:1173	End product tolerance of EG was also evident, as an activity of 228.57 IU mL-1 was observed in the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged.					
36152593	1	17	theme	thermostable	204:215	arg1	cellulases					217:226	cost effective and thermostable cellulases	185:226	cost effective and thermostable cellulases	185:226	Recent advancements in biorefinery processes necessitate search for cost effective and thermostable cellulases.					
36152593	1	18	theme	effective	190:198	arg1	cellulases					217:226	cost effective and thermostable cellulases	185:226	cost effective and thermostable cellulases	185:226	Recent advancements in biorefinery processes necessitate search for cost effective and thermostable cellulases.					
36152593	3	19	theme	methyltrioctylammonium	395:416	arg1	chloride					418:425	methyltrioctylammonium chloride	395:425	methyltrioctylammonium chloride	395:425	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	9	20	theme	mL-1	1175:1178	arg1	activity					1153:1160	an activity	1150:1160	an activity of 228.57 IU mL-1	1150:1178	End product tolerance of EG was also evident, as an activity of 228.57 IU mL-1 was observed in the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged.					
36152593	9	21	theme	IU	1172:1173	arg1	mL-1					1175:1178	228.57 IU mL-1	1165:1178	228.57 IU mL-1	1165:1178	End product tolerance of EG was also evident, as an activity of 228.57 IU mL-1 was observed in the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged.					
36152593	2	22	theme	thermophilic	299:310	arg1	UE25					353:356	Neobacillus sedimentimangrovi UE25	323:356	Neobacillus sedimentimangrovi UE25	323:356	This study was designed to characterize the cellulase obtained from a thermophilic bacterium, Neobacillus sedimentimangrovi UE25.					
36152593	2	22	theme	thermophilic	299:310	arg1	bacterium					312:320	a thermophilic bacterium	297:320	a thermophilic bacterium	297:320	This study was designed to characterize the cellulase obtained from a thermophilic bacterium, Neobacillus sedimentimangrovi UE25.					
36152593	8	23	contain	had	1066:1068	arg1	EG					1063:1064	The purified EG	1050:1064	The purified EG	1050:1064	The purified EG had a molecular weight of ∼45 kDa.					
36152593	8	23	contain	had	1066:1068	arg2	weight					1082:1087	a molecular weight	1070:1087	a molecular weight of ∼45 kDa	1070:1098	The purified EG had a molecular weight of ∼45 kDa.					
36152593	3	24	theme	sugarcane	440:448	arg1	SB					459:460	SB	459:460	SB	459:460	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	3	24	theme	sugarcane	440:448	arg1	bagasse					450:456	sugarcane bagasse	440:456	sugarcane bagasse (SB)	440:461	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	7	25	theme	DEAE	946:949	arg1	column					951:956	DEAE column	946:956	DEAE column	946:956	The purification of EG by using DEAE column yielded specific activity and purification fold of 365.866 IU mg-1 and 4.264, respectively.					
36152593	3	26	theme	lignin	467:472	arg1	removal					474:480	lignin removal	467:480	lignin removal	467:480	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	5	27	theme	detergents	840:849	arg1	presence					813:820	the presence	809:820	the presence of many salts and detergents	809:849	Cellulase showed its maximum activity at pH 7 and temperature 60 ℃ and it remained active in the presence of many salts and detergents.					
36152593	10	28	theme	36 µM	1403:1407	arg1	Vmax					1367:1370	Vmax	1367:1370	Vmax	1367:1370	The purified EG exhibited Vmax and Km of 294 U mL-1 min-1 and 36 µM, respectively, in the presence of 60 mM glucose.					
36152593	10	28	theme	36 µM	1403:1407	arg1	Km					1376:1377	Km	1376:1377	Km	1376:1377	The purified EG exhibited Vmax and Km of 294 U mL-1 min-1 and 36 µM, respectively, in the presence of 60 mM glucose.					
36152593	1	29	from	advancements	124:135	arg1	processes					152:160	biorefinery processes	140:160	biorefinery processes	140:160	Recent advancements in biorefinery processes necessitate search for cost effective and thermostable cellulases.					
36152593	0	30	theme	novel	22:26	arg1	product					32:38	a novel end product	20:38	a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25	20:114	Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25.					
36152593	7	31	theme	EG	934:935	arg1	purification					918:929	The purification	914:929	The purification of EG by using DEAE column	914:956	The purification of EG by using DEAE column yielded specific activity and purification fold of 365.866 IU mg-1 and 4.264, respectively.					
36152593	8	32	theme	molecular	1072:1080	arg1	weight					1082:1087	a molecular weight	1070:1087	a molecular weight of ∼45 kDa	1070:1098	The purified EG had a molecular weight of ∼45 kDa.					
36152593	11	33	theme	thermostable	1469:1480	arg1	cellulase					1482:1490	This novel thermostable cellulase	1458:1490	This novel thermostable cellulase	1458:1490	This novel thermostable cellulase can finds its applications in industrial sector.					
36152593	3	34	used	utilized	508:515	arg2	SB					501:502	the pretreated SB	486:502	the pretreated SB	486:502	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	3	34	used	utilized	508:515	arg2	source					529:534	a carbon source	520:534	a carbon source for the cellulase production	520:563	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	9	35	theme	end-product	1297:1307	arg1	accumulation					1281:1292	accumulation	1281:1292	accumulation of end-product	1281:1307	End product tolerance of EG was also evident, as an activity of 228.57 IU mL-1 was observed in the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged.					
36152593	9	36	theme	EG	1126:1127	arg1	tolerance					1113:1121	End product tolerance	1101:1121	End product tolerance of EG	1101:1127	End product tolerance of EG was also evident, as an activity of 228.57 IU mL-1 was observed in the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged.					
36152593	9	37	located	observed	1184:1191	arg1	presence					1200:1207	the presence	1196:1207	the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged	1196:1338	End product tolerance of EG was also evident, as an activity of 228.57 IU mL-1 was observed in the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged.					
36152593	9	37	located	observed	1184:1191	arg2	activity					1153:1160	an activity	1150:1160	an activity of 228.57 IU mL-1	1150:1178	End product tolerance of EG was also evident, as an activity of 228.57 IU mL-1 was observed in the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged.					
36152593	1	38	theme	Recent	117:122	arg1	advancements					124:135	Recent advancements	117:135	Recent advancements in biorefinery processes	117:160	Recent advancements in biorefinery processes necessitate search for cost effective and thermostable cellulases.					
36152593	9	39	theme	End	1101:1103	arg1	tolerance					1113:1121	End product tolerance	1101:1121	End product tolerance of EG	1101:1127	End product tolerance of EG was also evident, as an activity of 228.57 IU mL-1 was observed in the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged.					
36152593	0	40	theme	product	32:38	arg1	Characterization					0:15	Characterization	0:15	Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25.	0:115	Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25.					
36152593	9	41	theme	product	1105:1111	arg1	tolerance					1113:1121	End product tolerance	1101:1121	End product tolerance of EG	1101:1127	End product tolerance of EG was also evident, as an activity of 228.57 IU mL-1 was observed in the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged.					
36152593	3	42	theme	pretreated	490:499	arg1	SB					501:502	the pretreated SB	486:502	the pretreated SB	486:502	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	3	42	theme	pretreated	490:499	arg1	source					529:534	a carbon source	520:534	a carbon source for the cellulase production	520:563	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	0	43	theme	end	28:30	arg1	product					32:38	a novel end product	20:38	a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25	20:114	Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25.					
36152593	7	44	theme	mg-1	1020:1023	arg1	fold					1001:1004	purification fold	988:1004	purification fold	988:1004	The purification of EG by using DEAE column yielded specific activity and purification fold of 365.866 IU mg-1 and 4.264, respectively.					
36152593	7	44	theme	mg-1	1020:1023	arg1	activity					975:982	specific activity	966:982	specific activity	966:982	The purification of EG by using DEAE column yielded specific activity and purification fold of 365.866 IU mg-1 and 4.264, respectively.					
36152593	5	45	theme	many	825:828	arg1	salts					830:834	many salts	825:834	many salts	825:834	Cellulase showed its maximum activity at pH 7 and temperature 60 ℃ and it remained active in the presence of many salts and detergents.					
36152593	5	46	theme	salts	830:834	arg1	presence					813:820	the presence	809:820	the presence of many salts and detergents	809:849	Cellulase showed its maximum activity at pH 7 and temperature 60 ℃ and it remained active in the presence of many salts and detergents.					
36152593	0	47	theme	thermostable	53:64	arg1	cellulase					66:74	tolerant and thermostable cellulase	40:74	a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25	20:114	Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25.					
36152593	0	48	dep	product	32:38	arg1	cellulase					66:74	tolerant and thermostable cellulase	40:74	a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25	20:114	Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25.					
36152593	7	49	theme	4.264	1029:1033	arg1	fold					1001:1004	purification fold	988:1004	purification fold	988:1004	The purification of EG by using DEAE column yielded specific activity and purification fold of 365.866 IU mg-1 and 4.264, respectively.					
36152593	7	49	theme	4.264	1029:1033	arg1	activity					975:982	specific activity	966:982	specific activity	966:982	The purification of EG by using DEAE column yielded specific activity and purification fold of 365.866 IU mg-1 and 4.264, respectively.					
36152593	3	50	theme	carbon	522:527	arg1	SB					501:502	the pretreated SB	486:502	the pretreated SB	486:502	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	3	50	theme	carbon	522:527	arg1	source					529:534	a carbon source	520:534	a carbon source for the cellulase production	520:563	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	0	51	theme	tolerant	40:47	arg1	cellulase					66:74	tolerant and thermostable cellulase	40:74	a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25	20:114	Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25.					
36152593	8	52	theme	∼45 kDa	1092:1098	arg1	weight					1082:1087	a molecular weight	1070:1087	a molecular weight of ∼45 kDa	1070:1098	The purified EG had a molecular weight of ∼45 kDa.					
36152593	9	53	theme	60 mM	1212:1216	arg1	glucose					1218:1224	60 mM glucose	1212:1224	60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged	1212:1338	End product tolerance of EG was also evident, as an activity of 228.57 IU mL-1 was observed in the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged.					
36152593	7	54	theme	365.866	1009:1015	arg1	IU					1017:1018	IU	1017:1018	IU	1017:1018	The purification of EG by using DEAE column yielded specific activity and purification fold of 365.866 IU mg-1 and 4.264, respectively.					
36152593	4	55	theme	thermostable	570:581	arg1	cellulase					583:591	The thermostable cellulase	566:591	The thermostable cellulase thus obtained	566:605	The thermostable cellulase thus obtained was characterized by adopting central composite design which has not been reported earlier for this purpose.					
36152593	5	56	theme	maximum	737:743	arg1	activity					745:752	its maximum activity	733:752	its maximum activity	733:752	Cellulase showed its maximum activity at pH 7 and temperature 60 ℃ and it remained active in the presence of many salts and detergents.					
36152593	9	57	theme	glucose	1218:1224	arg1	presence					1200:1207	the presence	1196:1207	the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged	1196:1338	End product tolerance of EG was also evident, as an activity of 228.57 IU mL-1 was observed in the presence of 60 mM glucose which revealed that it does not lose its activity upon accumulation of end-product when the reaction is prolonged.					
36152593	7	58	theme	IU	1017:1018	arg1	mg-1					1020:1023	365.866 IU mg-1	1009:1023	365.866 IU mg-1	1009:1023	The purification of EG by using DEAE column yielded specific activity and purification fold of 365.866 IU mg-1 and 4.264, respectively.					
36152593	1	59	theme	biorefinery	140:150	arg1	processes					152:160	biorefinery processes	140:160	biorefinery processes	140:160	Recent advancements in biorefinery processes necessitate search for cost effective and thermostable cellulases.					
36152593	10	60	theme	60 mM	1443:1447	arg1	glucose					1449:1455	60 mM glucose	1443:1455	60 mM glucose	1443:1455	The purified EG exhibited Vmax and Km of 294 U mL-1 min-1 and 36 µM, respectively, in the presence of 60 mM glucose.					
36152593	0	61	from	sedimentimangrovi	93:109	arg1	product					32:38	a novel end product	20:38	a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25	20:114	Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25.					
36152593	0	61	from	sedimentimangrovi	93:109	arg1	Characterization					0:15	Characterization	0:15	Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25.	0:115	Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25.					
36152593	10	62	theme	glucose	1449:1455	arg1	presence					1431:1438	the presence	1427:1438	the presence of 60 mM glucose	1427:1455	The purified EG exhibited Vmax and Km of 294 U mL-1 min-1 and 36 µM, respectively, in the presence of 60 mM glucose.					
36152593	3	63	theme	cellulase	544:552	arg1	production					554:563	the cellulase production	540:563	the cellulase production	540:563	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
36152593	3	64	theme	NaOH	386:389	arg1	pretreatment					370:381	A combined pretreatment	359:381	A combined pretreatment of NaOH and methyltrioctylammonium chloride	359:425	A combined pretreatment of NaOH and methyltrioctylammonium chloride was given to sugarcane bagasse (SB) for lignin removal and the pretreated SB was utilized as a carbon source for the cellulase production.					
37083652	10	0	theme	LPS	2655:2657	arg1	levels					2683:2688	The LPS and inflammatory-factor levels	2651:2688	The LPS and inflammatory-factor levels	2651:2688	The LPS and inflammatory-factor levels were associated with an increased risk of poor functional outcomes at day 90.					
37083652	7	1	theme	good	1801:1804	arg1	group					1814:1818	the good outcome group	1797:1818	the good outcome group	1797:1818	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	2	theme	clinical	1405:1412	arg1	characteristics					1414:1428	the baseline demographic and clinical characteristics	1376:1428	characteristics	1414:1428	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	3	theme	moderate	1637:1644	arg1	stroke					1646:1651	a mild or moderate stroke	1627:1651	a mild or moderate stroke	1627:1651	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	3	theme	moderate	1637:1644	arg1	group					1729:1733	the severe group	1718:1733	the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group	1718:1888	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	4	theme	multiple	1352:1359	arg1	comparisons					1361:1371	multiple comparisons	1352:1371	multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group	1352:2021	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	5	theme	enzyme-linked	2244:2256	arg1	ELISA					2279:2283	ELISA	2279:2283	ELISA	2279:2283	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	8	5	theme	enzyme-linked	2244:2256	arg1	assay					2272:2276	an enzyme-linked immunosorbent assay	2241:2276	an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions	2241:2328	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	3	6	theme	gut	678:680	arg1	microbiota					682:691	different gut microbiota and inflammatory-factor levels	668:722	microbiota	682:691	Objective The study intended to determine if AIS patients would have different gut microbiota and inflammatory-factor levels than healthy individuals and if those levels would be associated with the stroke's severity and the patient's prognosis.					
37083652	7	7	theme	demographic	1389:1399	arg1	comparisons					1361:1371	multiple comparisons	1352:1371	multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group	1352:2021	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	2	8	theme	stroke	580:585	arg1	severities					587:596	different stroke severities	570:596	different stroke severities	570:596	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	7	9	theme	mild	1629:1632	arg1	stroke					1646:1651	a mild or moderate stroke	1627:1651	a mild or moderate stroke	1627:1651	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	9	theme	mild	1629:1632	arg1	group					1729:1733	the severe group	1718:1733	the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group	1718:1888	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	10	theme	stroke	2501:2506	arg1	severity					2489:2496	severity	2489:2496	severity of stroke and 90-day functional outcomes	2489:2537	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	8	11	theme	Outcome	2024:2030	arg1	Measures					2032:2039	Outcome Measures	2024:2039	Outcome Measures	2024:2039	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	8	12	theme	90-day	2512:2517	arg1	outcomes					2530:2537	90-day functional outcomes	2512:2537	90-day functional outcomes	2512:2537	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	7	13	theme	primary	1780:1786	arg1	outcome					1788:1794	a good primary outcome	1773:1794	a good primary outcome	1773:1794	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	11	14	theme	prognostic	2981:2990	arg1	markers					2992:2998	potential prognostic markers	2971:2998	potential prognostic markers	2971:2998	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	11	14	theme	prognostic	2981:2990	arg1	dysbiosis					2941:2949	dysbiosis	2941:2949	dysbiosis	2941:2949	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	7	15	contain	had	1697:1699	arg1	group					1729:1733	the severe group	1718:1733	the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group	1718:1888	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	15	contain	had	1697:1699	arg1	stroke					1646:1651	a mild or moderate stroke	1627:1651	a mild or moderate stroke	1627:1651	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	15	contain	had	1697:1699	arg2	stroke					1710:1715	a severe stroke	1701:1715	a severe stroke	1701:1715	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	15	contain	had	1697:1699	arg1	group					1672:1676	the mild-moderate group	1654:1676	the mild-moderate group	1654:1676	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	15	contain	had	1697:1699	arg1	those					1683:1687	those	1683:1687	those	1683:1687	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	16	theme	fecal	2455:2459	arg1	compositions					2471:2482	the fecal microbial compositions	2451:2482	the fecal microbial compositions	2451:2482	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	8	17	theme	fecal	2301:2305	arg1	compositions					2317:2328	the fecal microbial compositions	2297:2328	the fecal microbial compositions	2297:2328	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	1	18	theme	important	171:179	arg1	role					181:184	an important role	168:184	an important role	168:184	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	7	19	theme	AIS	1535:1537	arg1	group					1539:1543	the AIS group	1531:1543	the AIS group	1531:1543	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	19	theme	AIS	1535:1537	arg1	patients					1521:1528	AIS patients	1517:1528	AIS patients	1517:1528	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	10	20	theme	increased	2714:2722	arg1	risk					2724:2727	an increased risk	2711:2727	an increased risk of poor functional outcomes at day 90	2711:2765	The LPS and inflammatory-factor levels were associated with an increased risk of poor functional outcomes at day 90.					
37083652	11	21	theme	AIS	2860:2862	arg1	patients					2864:2871	AIS patients'	2860:2872	AIS patients' subsequent risks for poor functional outcomes	2860:2918	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	7	22	theme	outcome	1876:1882	arg1	group					1884:1888	the poor outcome group	1867:1888	the poor outcome group	1867:1888	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	22	theme	outcome	1876:1882	arg1	outcome					1858:1864	a poor primary outcome	1843:1864	a poor primary outcome	1843:1864	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	23	theme	compositions	2471:2482	arg1	associations					2435:2446	the associations	2431:2446	the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes	2431:2537	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	10	24	from	day	2760:2762	arg1	risk					2724:2727	an increased risk	2711:2727	an increased risk of poor functional outcomes at day 90	2711:2765	The LPS and inflammatory-factor levels were associated with an increased risk of poor functional outcomes at day 90.					
37083652	10	24	from	day	2760:2762	arg1	outcomes					2748:2755	poor functional outcomes	2732:2755	poor functional outcomes at day 90	2732:2765	The LPS and inflammatory-factor levels were associated with an increased risk of poor functional outcomes at day 90.					
37083652	5	25	theme	Electricity	1023:1033	arg1	Group					1035:1039	Electricity Group	1023:1039	Electricity Group	1023:1039	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	1	26	theme	ischemic	195:202	arg1	AIS					212:214	AIS	212:214	AIS	212:214	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	1	26	theme	ischemic	195:202	arg1	stroke					204:209	acute ischemic stroke	189:209	acute ischemic stroke (AIS)	189:215	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	7	27	theme	AIS	1517:1519	arg1	group					1539:1543	the AIS group	1531:1543	the AIS group	1531:1543	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	27	theme	AIS	1517:1519	arg1	group					1583:1587	the control group	1571:1587	the control group	1571:1587	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	27	theme	AIS	1517:1519	arg1	patients					1521:1528	AIS patients	1517:1528	AIS patients	1517:1528	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	2	28	theme	ischemic	542:549	arg1	AIS					559:561	AIS	559:561	AIS	559:561	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	2	28	theme	ischemic	542:549	arg1	stroke					551:556	acute ischemic stroke	536:556	acute ischemic stroke (AIS)	536:562	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	0	29	from	Changes	49:55	arg1	Outcomes					97:104	Functional Outcomes	86:104	Functional Outcomes	86:104	Ischemic Stroke and Dysbiosis of Gut Microbiota: Changes to LPS Levels and Effects on Functional Outcomes.					
37083652	7	30	contain	had	1839:1841	arg1	participants					1748:1759	AIS participants	1744:1759	AIS participants who had had a good primary outcome	1744:1794	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	30	contain	had	1839:1841	arg2	outcome					1858:1864	a poor primary outcome	1843:1864	a poor primary outcome	1843:1864	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	30	contain	had	1839:1841	arg2	group					1884:1888	the poor outcome group	1867:1888	the poor outcome group	1867:1888	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	30	contain	had	1839:1841	arg1	group					1814:1818	the good outcome group	1797:1818	the good outcome group	1797:1818	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	30	contain	had	1839:1841	arg1	those					1825:1829	those	1825:1829	those	1825:1829	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	3	31	theme	healthy	729:735	arg1	individuals					737:747	healthy individuals	729:747	healthy individuals	729:747	Objective The study intended to determine if AIS patients would have different gut microbiota and inflammatory-factor levels than healthy individuals and if those levels would be associated with the stroke's severity and the patient's prognosis.					
37083652	7	32	dep	factors	1478:1484	arg1	outcome					1970:1976	the good outcome	1961:1976	the good outcome	1961:1976	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	32	dep	factors	1478:1484	arg1	groups					1995:2000	poor outcome groups	1982:2000	poor outcome groups	1982:2000	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	11	33	theme	microbiota	2797:2806	arg1	levels					2840:2845	inflammatory-factor levels	2820:2845	inflammatory-factor levels	2820:2845	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	11	33	theme	microbiota	2797:2806	arg1	Dysbiosis					2780:2788	Conclusions Dysbiosis	2768:2788	Conclusions Dysbiosis of gut microbiota and LPS	2768:2814	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	8	34	theme	fecal	2087:2091	arg1	samples					2093:2099	participants' fecal samples	2073:2099	obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4)	2064:2419	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	7	35	theme	poor	1845:1848	arg1	group					1884:1888	the poor outcome group	1867:1888	the poor outcome group	1867:1888	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	35	theme	poor	1845:1848	arg1	outcome					1858:1864	a poor primary outcome	1843:1864	a poor primary outcome	1843:1864	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	5	36	theme	Coal	1014:1017	arg1	Hospital					1072:1079	the Third Affiliated Hospital	1051:1079	the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China	1051:1129	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	5	36	theme	Coal	1014:1017	arg1	Hospital					995:1002	the General Hospital	983:1002	the General Hospital	983:1002	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	5	37	dep	Setting	915:921	arg1	took					933:936	took	933:936	took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China	933:1129	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	8	38	theme	baseline	2145:2152	arg1	data					2154:2157	baseline data	2145:2157	(2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups	2131:2410	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	1	39	theme	vagal	296:300	arg1	pathways					321:328	immune, endocrine, vagal, and other humoral pathways	277:328	immune, endocrine, vagal, and other humoral pathways	277:328	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	7	40	dep	Groups	1317:1322	arg1	performed					1342:1350	performed	1342:1350	performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group	1342:2021	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	11	41	theme	inflammatory-factor	2820:2838	arg1	levels					2840:2845	inflammatory-factor levels	2820:2845	inflammatory-factor levels	2820:2845	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	7	42	theme	groups	1501:1506	arg1	number					1491:1496	a number	1489:1496	a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5)	1489:1959	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	2	43	theme	potential	450:458	arg1	associations					460:471	the potential associations	446:471	the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities	446:596	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	7	44	theme	control	2009:2015	arg1	group					2017:2021	the control group	2005:2021	the control group	2005:2021	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	0	45	theme	LPS	60:62	arg1	Levels					64:69	LPS Levels	60:69	LPS Levels	60:69	Ischemic Stroke and Dysbiosis of Gut Microbiota: Changes to LPS Levels and Effects on Functional Outcomes.					
37083652	5	46	theme	Bengbu	1084:1089	arg1	College					1099:1105	Bengbu Medical College	1084:1105	Bengbu Medical College	1084:1105	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	7	47	theme	AIS	1744:1746	arg1	participants					1748:1759	AIS participants	1744:1759	AIS participants who had had a good primary outcome	1744:1794	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	48	theme	control	1575:1581	arg1	group					1583:1587	the control group	1571:1587	the control group	1571:1587	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	48	theme	control	1575:1581	arg1	patients					1521:1528	AIS patients	1517:1528	AIS patients	1517:1528	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	49	theme	number	1491:1496	arg1	factors					1478:1484	inflammatory factors	1465:1484	inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups	1465:2000	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	2	50	theme	stroke	495:500	arg1	outcomes					502:509	stroke outcomes	495:509	stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities	495:596	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	8	51	theme	lipopolysaccharide	2331:2348	arg1	contents					2356:2363	lipopolysaccharide (LPS) contents	2331:2363	lipopolysaccharide (LPS) contents	2331:2363	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	1	52	dep	Context	107:113	arg1	microbiota					130:139	The intestinal microbiota	115:139	The intestinal microbiota	115:139	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	8	53	theme	included	2167:2174	arg1	patients					2180:2187	the included AIS patients	2163:2187	the included AIS patients	2163:2187	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	4	54	dep	prospective	882:892	arg1	observational					894:906	observational	894:906	observational	894:906	Design The research team performed a prospective observational study.					
37083652	1	55	theme	humoral	313:319	arg1	pathways					321:328	immune, endocrine, vagal, and other humoral pathways	277:328	immune, endocrine, vagal, and other humoral pathways	277:328	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	10	56	theme	functional	2737:2746	arg1	outcomes					2748:2755	poor functional outcomes	2732:2755	poor functional outcomes at day 90	2732:2765	The LPS and inflammatory-factor levels were associated with an increased risk of poor functional outcomes at day 90.					
37083652	2	57	theme	factors	482:488	arg1	associations					460:471	the potential associations	446:471	the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities	446:596	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	2	57	theme	factors	482:488	arg1	factors					435:441	inflammatory factors	422:441	inflammatory factors	422:441	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	7	58	theme	outcome	1987:1993	arg1	groups					1995:2000	poor outcome groups	1982:2000	poor outcome groups	1982:2000	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	59	theme	5	1958:1958	arg1	number					1491:1496	a number	1489:1496	a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5)	1489:1959	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	11	60	theme	LPS	2812:2814	arg1	levels					2840:2845	inflammatory-factor levels	2820:2845	inflammatory-factor levels	2820:2845	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	11	60	theme	LPS	2812:2814	arg1	Dysbiosis					2780:2788	Conclusions Dysbiosis	2768:2788	Conclusions Dysbiosis of gut microbiota and LPS	2768:2814	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	5	61	theme	Third	1055:1059	arg1	Hospital					1072:1079	the Third Affiliated Hospital	1051:1079	the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China	1051:1129	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	5	61	theme	Third	1055:1059	arg1	Hospital					995:1002	the General Hospital	983:1002	the General Hospital	983:1002	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	2	62	dep	microbiome	393:402	arg1	the					385:387	the	385:387	the	385:387	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	7	63	theme	healthy	1553:1559	arg1	controls					1561:1568	the healthy controls	1549:1568	the healthy controls	1549:1568	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	0	64	theme	Ischemic	0:7	arg1	Stroke					9:14	Ischemic Stroke	0:14	Ischemic Stroke	0:14	Ischemic Stroke and Dysbiosis of Gut Microbiota: Changes to LPS Levels and Effects on Functional Outcomes.					
37083652	8	65	with	associations	2435:2446	arg1	severity					2489:2496	severity	2489:2496	severity of stroke and 90-day functional outcomes	2489:2537	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	7	66	dep	participants	1748:1759	arg1	3					1737:1737	3	1737:1737	3	1737:1737	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	11	67	theme	Conclusions	2768:2778	arg1	Dysbiosis					2780:2788	Conclusions Dysbiosis	2768:2788	Conclusions Dysbiosis of gut microbiota and LPS	2768:2814	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	7	68	contain	had	1623:1625	arg2	those					1683:1687	those	1683:1687	those	1683:1687	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	68	contain	had	1623:1625	arg2	stroke					1646:1651	a mild or moderate stroke	1627:1651	a mild or moderate stroke	1627:1651	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	68	contain	had	1623:1625	arg1	participants					1602:1613	AIS participants	1598:1613	(2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4)	1590:1893	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	68	contain	had	1623:1625	arg2	group					1672:1676	the mild-moderate group	1654:1676	the mild-moderate group	1654:1676	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	68	contain	had	1623:1625	arg2	group					1729:1733	the severe group	1718:1733	the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group	1718:1888	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	2	69	theme	gut	389:391	arg1	microbiome					393:402	gut microbiome	389:402	gut microbiome	389:402	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	9	70	dep	Results	2574:2580	arg1	microflora					2590:2599	The gut microflora	2582:2599	Results The gut microflora	2574:2599	Results The gut microflora distinguished AIS patients from healthy controls.					
37083652	1	71	theme	immune	277:282	arg1	pathways					321:328	immune, endocrine, vagal, and other humoral pathways	277:328	immune, endocrine, vagal, and other humoral pathways	277:328	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	7	72	dep	group	1729:1733	arg1	participants					1748:1759	AIS participants	1744:1759	AIS participants who had had a good primary outcome	1744:1794	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	72	dep	group	1729:1733	arg1	group					1814:1818	the good outcome group	1797:1818	the good outcome group	1797:1818	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	72	dep	group	1729:1733	arg1	those					1825:1829	those	1825:1829	those	1825:1829	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	11	73	theme	therapeutic	3004:3014	arg1	targets					3016:3022	therapeutic targets	3004:3022	therapeutic targets for stroke	3004:3033	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	2	74	theme	inflammatory	422:433	arg1	factors					435:441	inflammatory factors	422:441	inflammatory factors	422:441	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	5	75	theme	College	1099:1105	arg1	Hospital					1072:1079	the Third Affiliated Hospital	1051:1079	the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China	1051:1129	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	5	75	theme	College	1099:1105	arg1	Hospital					995:1002	the General Hospital	983:1002	the General Hospital	983:1002	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	8	76	theme	16S	2212:2214	arg1	sequencing					2226:2235	16S rRNA gene sequencing	2212:2235	16S rRNA gene sequencing	2212:2235	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	0	77	theme	Microbiota	37:46	arg1	Dysbiosis					20:28	Dysbiosis	20:28	Dysbiosis	20:28	Ischemic Stroke and Dysbiosis of Gut Microbiota: Changes to LPS Levels and Effects on Functional Outcomes.					
37083652	0	77	theme	Microbiota	37:46	arg1	Stroke					9:14	Ischemic Stroke	0:14	Ischemic Stroke	0:14	Ischemic Stroke and Dysbiosis of Gut Microbiota: Changes to LPS Levels and Effects on Functional Outcomes.					
37083652	1	78	theme	endocrine	285:293	arg1	pathways					321:328	immune, endocrine, vagal, and other humoral pathways	277:328	immune, endocrine, vagal, and other humoral pathways	277:328	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	7	79	dep	patients	1521:1528	arg1	1					1510:1510	1	1510:1510	1	1510:1510	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	79	dep	patients	1521:1528	arg1	participants					1602:1613	AIS participants	1598:1613	(2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4)	1590:1893	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	79	dep	patients	1521:1528	arg1	groups					1924:1929	mild-moderate and severe groups	1899:1929	(1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group	1509:1950	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	80	theme	microbiota	1439:1448	arg1	comparisons					1361:1371	multiple comparisons	1352:1371	multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group	1352:2021	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	11	81	theme	poor	2895:2898	arg1	outcomes					2911:2918	poor functional outcomes	2895:2918	poor functional outcomes	2895:2918	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	8	82	theme	gene	2221:2224	arg1	sequencing					2226:2235	16S rRNA gene sequencing	2212:2235	16S rRNA gene sequencing	2212:2235	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	8	83	dep	data	2154:2157	arg1	used					2207:2210	used	2207:2210	used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups	2207:2410	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	8	83	dep	data	2154:2157	arg1	2					2132:2132	2	2132:2132	2	2132:2132	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	2	84	from	outcomes	502:509	arg1	patients					514:521	patients	514:521	patients who have had acute ischemic stroke (AIS), with different stroke severities	514:596	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	10	85	from	risk	2724:2727	arg1	day					2760:2762	day 90	2760:2765	day 90	2760:2765	The LPS and inflammatory-factor levels were associated with an increased risk of poor functional outcomes at day 90.					
37083652	3	86	dep	Objective	599:607	arg1	intended					619:626	intended	619:626	intended to determine if AIS patients would have different gut microbiota and inflammatory-factor levels than healthy individuals and if those levels would be associated with the stroke's severity and the patient's prognosis	619:842	Objective The study intended to determine if AIS patients would have different gut microbiota and inflammatory-factor levels than healthy individuals and if those levels would be associated with the stroke's severity and the patient's prognosis.					
37083652	7	87	theme	characteristics	1414:1428	arg1	comparisons					1361:1371	multiple comparisons	1352:1371	multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group	1352:2021	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	5	88	theme	Rehabilitation	965:978	arg1	Department					951:960	the Department	947:960	the Department of Rehabilitation	947:978	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	7	89	dep	participants	1602:1613	arg1	2					1591:1591	2	1591:1591	2	1591:1591	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	90	theme	admission	2120:2128	arg1	hours					2111:2115	72 hours	2108:2115	72 hours of admission	2108:2128	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	3	91	theme	different	668:676	arg1	microbiota					682:691	different gut microbiota and inflammatory-factor levels	668:722	microbiota	682:691	Objective The study intended to determine if AIS patients would have different gut microbiota and inflammatory-factor levels than healthy individuals and if those levels would be associated with the stroke's severity and the patient's prognosis.					
37083652	8	92	dep	used	2207:2210	arg1	3					2204:2204	3	2204:2204	3	2204:2204	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	3	93	theme	AIS	644:646	arg1	patients					648:655	AIS patients	644:655	AIS patients	644:655	Objective The study intended to determine if AIS patients would have different gut microbiota and inflammatory-factor levels than healthy individuals and if those levels would be associated with the stroke's severity and the patient's prognosis.					
37083652	6	94	theme	AIS	1221:1223	arg1	treatment					1208:1216	treatment	1208:1216	treatment	1208:1216	Participants Participants were 90 patients who had received a diagnosis and treatment of AIS within 48 hours of the stroke's onset at the hospital, between October 2021 and March 2022.					
37083652	6	94	theme	AIS	1221:1223	arg1	diagnosis					1194:1202	diagnosis	1194:1202	diagnosis	1194:1202	Participants Participants were 90 patients who had received a diagnosis and treatment of AIS within 48 hours of the stroke's onset at the hospital, between October 2021 and March 2022.					
37083652	1	95	theme	brain	230:234	arg1	functions					236:244	brain functions	230:244	brain functions	230:244	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	7	96	theme	outcome	1806:1812	arg1	group					1814:1818	the good outcome group	1797:1818	the good outcome group	1797:1818	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	97	theme	research	2045:2052	arg1	team					2054:2057	The research team	2041:2057	The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.	2041:2572	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	8	98	dep	hours	2111:2115	arg1	data					2154:2157	baseline data	2145:2157	(2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups	2131:2410	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	8	99	theme	functional	2519:2528	arg1	outcomes					2530:2537	90-day functional outcomes	2512:2537	90-day functional outcomes	2512:2537	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	11	100	theme	potential	2971:2979	arg1	markers					2992:2998	potential prognostic markers	2971:2998	potential prognostic markers	2971:2998	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	11	100	theme	potential	2971:2979	arg1	dysbiosis					2941:2949	dysbiosis	2941:2949	dysbiosis	2941:2949	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	2	101	theme	different	570:578	arg1	severities					587:596	different stroke severities	570:596	different stroke severities	570:596	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	10	102	theme	poor	2732:2735	arg1	outcomes					2748:2755	poor functional outcomes	2732:2755	poor functional outcomes at day 90	2732:2765	The LPS and inflammatory-factor levels were associated with an increased risk of poor functional outcomes at day 90.					
37083652	8	103	theme	microbial	2461:2469	arg1	compositions					2471:2482	the fecal microbial compositions	2451:2482	the fecal microbial compositions	2451:2482	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	4	104	dep	Design	845:850	arg1	team					865:868	The research team	852:868	Design The research team	845:868	Design The research team performed a prospective observational study.					
37083652	7	105	theme	research	1328:1335	arg1	team					1337:1340	The research team	1324:1340	The research team	1324:1340	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	106	theme	logistic-regression	2546:2564	arg1	models					2566:2571	logistic-regression models	2546:2571	logistic-regression models	2546:2571	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	7	107	theme	good	1775:1778	arg1	outcome					1788:1794	a good primary outcome	1773:1794	a good primary outcome	1773:1794	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	108	theme	obtained	2064:2071	arg1	samples					2093:2099	participants' fecal samples	2073:2099	obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4)	2064:2419	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	3	109	theme	inflammatory-factor	697:715	arg1	levels					717:722	different gut microbiota and inflammatory-factor levels	668:722	levels	717:722	Objective The study intended to determine if AIS patients would have different gut microbiota and inflammatory-factor levels than healthy individuals and if those levels would be associated with the stroke's severity and the patient's prognosis.					
37083652	6	110	theme	onset	1257:1261	arg1	hours					1235:1239	48 hours	1232:1239	48 hours of the stroke's onset at the hospital	1232:1277	Participants Participants were 90 patients who had received a diagnosis and treatment of AIS within 48 hours of the stroke's onset at the hospital, between October 2021 and March 2022.					
37083652	5	111	theme	Group	1035:1039	arg1	Hospital					1072:1079	the Third Affiliated Hospital	1051:1079	the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China	1051:1129	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	5	111	theme	Group	1035:1039	arg1	Hospital					995:1002	the General Hospital	983:1002	the General Hospital	983:1002	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	8	112	theme	outcomes	2530:2537	arg1	severity					2489:2496	severity	2489:2496	severity of stroke and 90-day functional outcomes	2489:2537	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	1	113	theme	acute	189:193	arg1	AIS					212:214	AIS	212:214	AIS	212:214	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	1	113	theme	acute	189:193	arg1	stroke					204:209	acute ischemic stroke	189:209	acute ischemic stroke (AIS)	189:215	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	4	114	theme	prospective	882:892	arg1	study					908:912	a prospective observational study	880:912	a prospective observational study	880:912	Design The research team performed a prospective observational study.					
37083652	7	115	theme	poor	1871:1874	arg1	group					1884:1888	the poor outcome group	1867:1888	the poor outcome group	1867:1888	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	115	theme	poor	1871:1874	arg1	outcome					1858:1864	a poor primary outcome	1843:1864	a poor primary outcome	1843:1864	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	5	116	theme	Medical	1091:1097	arg1	College					1099:1105	Bengbu Medical College	1084:1105	Bengbu Medical College	1084:1105	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	2	117	theme	acute	536:540	arg1	AIS					559:561	AIS	559:561	AIS	559:561	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	2	117	theme	acute	536:540	arg1	stroke					551:556	acute ischemic stroke	536:556	acute ischemic stroke (AIS)	536:562	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	3	118	contain	have	663:666	arg1	patients					648:655	AIS patients	644:655	AIS patients	644:655	Objective The study intended to determine if AIS patients would have different gut microbiota and inflammatory-factor levels than healthy individuals and if those levels would be associated with the stroke's severity and the patient's prognosis.					
37083652	3	118	contain	have	663:666	arg2	microbiota					682:691	different gut microbiota and inflammatory-factor levels	668:722	microbiota	682:691	Objective The study intended to determine if AIS patients would have different gut microbiota and inflammatory-factor levels than healthy individuals and if those levels would be associated with the stroke's severity and the patient's prognosis.					
37083652	3	118	contain	have	663:666	arg2	levels					717:722	different gut microbiota and inflammatory-factor levels	668:722	levels	717:722	Objective The study intended to determine if AIS patients would have different gut microbiota and inflammatory-factor levels than healthy individuals and if those levels would be associated with the stroke's severity and the patient's prognosis.					
37083652	8	119	theme	collected	2135:2143	arg1	data					2154:2157	baseline data	2145:2157	(2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups	2131:2410	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	11	120	theme	subsequent	2874:2883	arg1	risks					2885:2889	AIS patients' subsequent risks	2860:2889	AIS patients' subsequent risks for poor functional outcomes	2860:2918	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	5	121	theme	Wanbei	1007:1012	arg1	Coal					1014:1017	Wanbei Coal	1007:1017	Wanbei Coal	1007:1017	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	7	122	theme	AIS	1598:1600	arg1	participants					1602:1613	AIS participants	1598:1613	(2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4)	1590:1893	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	4	123	theme	research	856:863	arg1	team					865:868	The research team	852:868	Design The research team	845:868	Design The research team performed a prospective observational study.					
37083652	0	124	from	Effects	75:81	arg1	Outcomes					97:104	Functional Outcomes	86:104	Functional Outcomes	86:104	Ischemic Stroke and Dysbiosis of Gut Microbiota: Changes to LPS Levels and Effects on Functional Outcomes.					
37083652	7	125	theme	primary	1850:1856	arg1	group					1884:1888	the poor outcome group	1867:1888	the poor outcome group	1867:1888	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	125	theme	primary	1850:1856	arg1	outcome					1858:1864	a poor primary outcome	1843:1864	a poor primary outcome	1843:1864	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	126	dep	team	2054:2057	arg1	evaluated					2421:2429	evaluated	2421:2429	evaluated	2421:2429	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	8	126	dep	team	2054:2057	arg1	Measures					2032:2039	Outcome Measures	2024:2039	Outcome Measures	2024:2039	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	1	127	theme	intestinal	119:128	arg1	microbiota					130:139	The intestinal microbiota	115:139	The intestinal microbiota	115:139	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	11	128	theme	gut	2793:2795	arg1	microbiota					2797:2806	gut microbiota	2793:2806	gut microbiota	2793:2806	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	2	129	theme	associations	460:471	arg1	microbiome					393:402	gut microbiome	389:402	gut microbiome	389:402	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	2	129	theme	associations	460:471	arg1	levels					412:417	its levels	408:417	its levels	408:417	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	6	130	theme	Participants	1132:1143	arg1	Participants					1145:1156	Participants Participants	1132:1156	Participants Participants	1132:1156	Participants Participants were 90 patients who had received a diagnosis and treatment of AIS within 48 hours of the stroke's onset at the hospital, between October 2021 and March 2022.					
37083652	2	131	with	factors	435:441	arg1	outcomes					502:509	stroke outcomes	495:509	stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities	495:596	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	9	132	theme	gut	2586:2588	arg1	microflora					2590:2599	The gut microflora	2582:2599	Results The gut microflora	2574:2599	Results The gut microflora distinguished AIS patients from healthy controls.					
37083652	8	133	theme	microbial	2307:2315	arg1	compositions					2317:2328	the fecal microbial compositions	2297:2328	the fecal microbial compositions	2297:2328	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	8	134	theme	AIS	2176:2178	arg1	patients					2180:2187	the included AIS patients	2163:2187	the included AIS patients	2163:2187	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	1	135	theme	other	307:311	arg1	pathways					321:328	immune, endocrine, vagal, and other humoral pathways	277:328	immune, endocrine, vagal, and other humoral pathways	277:328	Context The intestinal microbiota and their metabolites play an important role in acute ischemic stroke (AIS) and modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways.					
37083652	10	136	theme	outcomes	2748:2755	arg1	risk					2724:2727	an increased risk	2711:2727	an increased risk of poor functional outcomes at day 90	2711:2765	The LPS and inflammatory-factor levels were associated with an increased risk of poor functional outcomes at day 90.					
37083652	7	137	theme	factors	1478:1484	arg1	demographic					1389:1399	demographic	1389:1399	demographic	1389:1399	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	138	link	enzyme-linked	2244:2256	arg1	ELISA					2279:2283	ELISA	2279:2283	ELISA	2279:2283	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	8	138	link	enzyme-linked	2244:2256	arg1	assay					2272:2276	an enzyme-linked immunosorbent assay	2241:2276	an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions	2241:2328	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	7	139	theme	poor	1982:1985	arg1	groups					1995:2000	poor outcome groups	1982:2000	poor outcome groups	1982:2000	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	140	contain	had	1769:1771	arg2	outcome					1788:1794	a good primary outcome	1773:1794	a good primary outcome	1773:1794	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	140	contain	had	1769:1771	arg1	participants					1748:1759	AIS participants	1744:1759	AIS participants who had had a good primary outcome	1744:1794	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	140	contain	had	1769:1771	arg1	group					1814:1818	the good outcome group	1797:1818	the good outcome group	1797:1818	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	140	contain	had	1769:1771	arg1	those					1825:1829	those	1825:1829	those	1825:1829	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	141	theme	good	1965:1968	arg1	outcome					1970:1976	the good outcome	1961:1976	the good outcome	1961:1976	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	0	142	theme	Functional	86:95	arg1	Outcomes					97:104	Functional Outcomes	86:104	Functional Outcomes	86:104	Ischemic Stroke and Dysbiosis of Gut Microbiota: Changes to LPS Levels and Effects on Functional Outcomes.					
37083652	6	143	from	hospital	1270:1277	arg1	onset					1257:1261	the stroke's onset	1244:1261	the stroke's onset at the hospital	1244:1277	Participants Participants were 90 patients who had received a diagnosis and treatment of AIS within 48 hours of the stroke's onset at the hospital, between October 2021 and March 2022.					
37083652	6	143	from	hospital	1270:1277	arg1	hours					1235:1239	48 hours	1232:1239	48 hours of the stroke's onset at the hospital	1232:1277	Participants Participants were 90 patients who had received a diagnosis and treatment of AIS within 48 hours of the stroke's onset at the hospital, between October 2021 and March 2022.					
37083652	8	144	dep	evaluated	2421:2429	arg1	1					2061:2061	1	2061:2061	1	2061:2061	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	2	145	with	associations	460:471	arg1	outcomes					502:509	stroke outcomes	495:509	stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities	495:596	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	2	146	theme	few	351:353	arg1	investigations					355:368	relatively few investigations	340:368	relatively few investigations	340:368	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	5	147	theme	Affiliated	1061:1070	arg1	Hospital					1072:1079	the Third Affiliated Hospital	1051:1079	the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China	1051:1129	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	5	147	theme	Affiliated	1061:1070	arg1	Hospital					995:1002	the General Hospital	983:1002	the General Hospital	983:1002	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	7	148	theme	severe	1722:1727	arg1	stroke					1646:1651	a mild or moderate stroke	1627:1651	a mild or moderate stroke	1627:1651	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	148	theme	severe	1722:1727	arg1	group					1729:1733	the severe group	1718:1733	the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group	1718:1888	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	9	149	theme	AIS	2615:2617	arg1	patients					2619:2626	AIS patients	2615:2626	AIS patients	2615:2626	Results The gut microflora distinguished AIS patients from healthy controls.					
37083652	9	150	theme	healthy	2633:2639	arg1	controls					2641:2648	healthy controls	2633:2648	healthy controls	2633:2648	Results The gut microflora distinguished AIS patients from healthy controls.					
37083652	7	151	dep	groups	1501:1506	arg1	group					1539:1543	the AIS group	1531:1543	the AIS group	1531:1543	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	151	dep	groups	1501:1506	arg1	group					1583:1587	the control group	1571:1587	the control group	1571:1587	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	151	dep	groups	1501:1506	arg1	patients					1521:1528	AIS patients	1517:1528	AIS patients	1517:1528	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	10	152	theme	inflammatory-factor	2663:2681	arg1	levels					2683:2688	The LPS and inflammatory-factor levels	2651:2688	The LPS and inflammatory-factor levels	2651:2688	The LPS and inflammatory-factor levels were associated with an increased risk of poor functional outcomes at day 90.					
37083652	0	153	theme	Gut	33:35	arg1	Microbiota					37:46	Gut Microbiota	33:46	Gut Microbiota	33:46	Ischemic Stroke and Dysbiosis of Gut Microbiota: Changes to LPS Levels and Effects on Functional Outcomes.					
37083652	7	154	theme	severe	1703:1708	arg1	stroke					1710:1715	a severe stroke	1701:1715	a severe stroke	1701:1715	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	155	theme	inflammatory	1465:1476	arg1	factors					1478:1484	inflammatory factors	1465:1484	inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups	1465:2000	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	156	dep	assay	2272:2276	arg1	compare					2289:2295	compare	2289:2295	to compare the fecal microbial compositions	2286:2328	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	2	157	theme	factors	435:441	arg1	microbiome					393:402	gut microbiome	389:402	gut microbiome	389:402	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	2	157	theme	factors	435:441	arg1	levels					412:417	its levels	408:417	its levels	408:417	However, relatively few investigations have evaluated the gut microbiome and its levels of inflammatory factors or the potential associations of those factors with stroke outcomes in patients who have had acute ischemic stroke (AIS), with different stroke severities.					
37083652	8	158	theme	inflammatory-factor	2370:2388	arg1	levels					2390:2395	inflammatory-factor levels	2370:2395	inflammatory-factor levels between groups	2370:2410	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	7	159	theme	control	1938:1944	arg1	group					1946:1950	the control group	1934:1950	the control group	1934:1950	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	160	theme	rRNA	2216:2219	arg1	sequencing					2226:2235	16S rRNA gene sequencing	2212:2235	16S rRNA gene sequencing	2212:2235	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	5	161	from	Hospital	1072:1079	arg1	China					1125:1129	China	1125:1129	China	1125:1129	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	5	161	from	Hospital	1072:1079	arg1	 Anhui					1117:1122	 Anhui	1117:1122	 Anhui	1117:1122	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	6	162	from	hours	1235:1239	arg1	hospital					1270:1277	the hospital	1266:1277	the hospital	1266:1277	Participants Participants were 90 patients who had received a diagnosis and treatment of AIS within 48 hours of the stroke's onset at the hospital, between October 2021 and March 2022.					
37083652	7	163	theme	levels	1455:1460	arg1	comparisons					1361:1371	multiple comparisons	1352:1371	multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group	1352:2021	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	5	164	theme	General	987:993	arg1	Hospital					1072:1079	the Third Affiliated Hospital	1051:1079	the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China	1051:1129	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	5	164	theme	General	987:993	arg1	Hospital					995:1002	the General Hospital	983:1002	the General Hospital	983:1002	Setting The study took place in the Department of Rehabilitation at the General Hospital of Wanbei Coal and Electricity Group, which is the Third Affiliated Hospital of Bengbu Medical College in Suzhou, Anhui, China.					
37083652	11	165	theme	functional	2900:2909	arg1	outcomes					2911:2918	poor functional outcomes	2895:2918	poor functional outcomes	2895:2918	Conclusions Dysbiosis of gut microbiota and LPS and inflammatory-factor levels can increase AIS patients' subsequent risks for poor functional outcomes, indicating that the dysbiosis and levels could be potential prognostic markers and therapeutic targets for stroke.					
37083652	7	166	theme	severe	1917:1922	arg1	groups					1924:1929	mild-moderate and severe groups	1899:1929	(1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group	1509:1950	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	6	167	dep	diagnosis	1194:1202	arg1	a					1192:1192	a	1192:1192	a	1192:1192	Participants Participants were 90 patients who had received a diagnosis and treatment of AIS within 48 hours of the stroke's onset at the hospital, between October 2021 and March 2022.					
37083652	7	168	theme	gut	1435:1437	arg1	microbiota					1439:1448	the gut microbiota	1431:1448	the gut microbiota	1431:1448	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	0	169	dep	Stroke	9:14	arg1	Effects					75:81	Effects	75:81	Effects on Functional Outcomes	75:104	Ischemic Stroke and Dysbiosis of Gut Microbiota: Changes to LPS Levels and Effects on Functional Outcomes.					
37083652	0	169	dep	Stroke	9:14	arg1	Changes					49:55	Changes	49:55	Changes to LPS Levels	49:69	Ischemic Stroke and Dysbiosis of Gut Microbiota: Changes to LPS Levels and Effects on Functional Outcomes.					
37083652	7	170	theme	mild-moderate	1658:1670	arg1	group					1672:1676	the mild-moderate group	1654:1676	the mild-moderate group	1654:1676	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	7	171	theme	mild-moderate	1899:1911	arg1	groups					1924:1929	mild-moderate and severe groups	1899:1929	(1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group	1509:1950	Groups The research team performed multiple comparisons of the baseline demographic and clinical characteristics, the gut microbiota, and levels of inflammatory factors of a number of groups: (1) the AIS patients, the AIS group, to the healthy controls, the control group; (2) the AIS participants who had had a mild or moderate stroke, the mild-moderate group, and those who had had a severe stroke, the severe group; (3) the AIS participants who had had a good primary outcome, the good outcome group, and those who had had a poor primary outcome, the poor outcome group; (4) the mild-moderate and severe groups to the control group; and (5) the good outcome and poor outcome groups to the control group.					
37083652	8	172	theme	immunosorbent	2258:2270	arg1	ELISA					2279:2283	ELISA	2279:2283	ELISA	2279:2283	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
37083652	8	172	theme	immunosorbent	2258:2270	arg1	assay					2272:2276	an enzyme-linked immunosorbent assay	2241:2276	an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions	2241:2328	Outcome Measures The research team: (1) obtained participants' fecal samples within 72 hours of admission; (2) collected baseline data for the included AIS patients and controls; (3) used 16S rRNA gene sequencing and an enzyme-linked immunosorbent assay (ELISA) to compare the fecal microbial compositions, lipopolysaccharide (LPS) contents, and inflammatory-factor levels between groups; and (4) evaluated the associations of the fecal microbial compositions with severity of stroke and 90-day functional outcomes, using logistic-regression models.					
36038978	2	0	theme	photocatalytic	470:483	arg1	removals					500:507	The photocatalytic and adsorptive removals	466:507	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite)	466:646	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	2	1	dep	mg	557:558	arg1	10					554:555	10	554:555	10	554:555	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	2	1	dep	mg	557:558	arg1	1					548:548	1	548:548	1	548:548	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	2	2	theme	composite	637:645	arg1	L-1					630:632	0.3 g L-1	624:632	0.3 g L-1 of composite	624:645	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	2	2	theme	composite	637:645	arg1	power					617:621	32 W UV-C power	607:621	32 W UV-C power	607:621	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	3	3	theme	adsorption	764:773	arg1	CEF					791:793	MNZ > TET > CEF	779:793	MNZ > TET > CEF	779:793	Antibiotics adsorption followed pseudo-second-order kinetics, and the order of adsorption was MNZ > TET > CEF.					
36038978	3	3	theme	adsorption	764:773	arg1	order					755:759	the order	751:759	the order of adsorption	751:773	Antibiotics adsorption followed pseudo-second-order kinetics, and the order of adsorption was MNZ > TET > CEF.					
36038978	4	4	theme	TET	887:889	arg1	drugs					891:895	hydrophobic CEF and TET drugs	867:895	hydrophobic CEF and TET drugs	867:895	On the other hand, the hydrophilic MNZ was degraded faster compared to hydrophobic CEF and TET drugs.					
36038978	8	5	theme	structural	1384:1393	arg1	stability					1395:1403	structural stability	1384:1403	structural stability	1384:1403	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	8	6	theme	consistent	1406:1415	arg1	performance					1417:1427	consistent performance	1406:1427	consistent performance	1406:1427	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	8	7	theme	metallic	1534:1541	arg1	strainer					1547:1554	metallic tea strainer	1534:1554	metallic tea strainer	1534:1554	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	6	8	dep	mg	1165:1166	arg1	L-1					1168:1170	L-1	1168:1170	668.6 mg L-1	1159:1170	Micro-toxicity of antibiotics was performed using bioluminescent bacterium Vibrio fischeri and EC50 of CEF, TET and MNZ were found to be 18.25 mg L-1, 173.8 mg L-1, and 668.6 mg L-1, respectively.					
36038978	7	9	theme	toxicity	1209:1216	arg1	levels					1218:1223	the relative toxicity levels	1196:1223	the relative toxicity levels of PVA-CS-TiO2 and treated effluent	1196:1259	However, the relative toxicity levels of PVA-CS-TiO2 and treated effluent were well with the limits as inferred from the microtoxicity analysis.					
36038978	7	10	theme	effluent	1252:1259	arg1	levels					1218:1223	the relative toxicity levels	1196:1223	the relative toxicity levels of PVA-CS-TiO2 and treated effluent	1196:1259	However, the relative toxicity levels of PVA-CS-TiO2 and treated effluent were well with the limits as inferred from the microtoxicity analysis.					
36038978	2	11	from	composite	572:580	arg1	system					599:604	an UV reactor system	585:604	an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite)	585:646	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	8	12	theme	tea	1543:1545	arg1	strainer					1547:1554	metallic tea strainer	1534:1554	metallic tea strainer	1534:1554	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	8	13	from	loading	1503:1509	arg1	antibiotics					1469:1479	all antibiotics	1465:1479	all antibiotics at a minimal catalyst loading	1465:1509	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	0	14	theme	single-	112:118	arg1	conditions					140:149	single- and multi-pollutant conditions	112:149	Photocatalytic and adsorptive performance of polyvinyl alcohol/chitosan/TiO2 composite for antibiotics removal: single- and multi-pollutant conditions.	0:150	Photocatalytic and adsorptive performance of polyvinyl alcohol/chitosan/TiO2 composite for antibiotics removal: single- and multi-pollutant conditions.					
36038978	2	15	theme	L-1	560:562	arg1	concentrations					525:538	concentrations	525:538	concentrations of 0.1, 1 and 10 mg L-1	525:562	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	1	16	theme	single	419:424	arg1	compound					426:433	single compound	419:433	single compound	419:433	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	1	16	theme	single	419:424	arg1	composite					173:181	A polymer-TiO2 macro composite	152:181	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2)	152:201	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	6	17	theme	MNZ	1106:1108	arg1	EC50					1085:1088	EC50	1085:1088	EC50 of CEF, TET and MNZ	1085:1108	Micro-toxicity of antibiotics was performed using bioluminescent bacterium Vibrio fischeri and EC50 of CEF, TET and MNZ were found to be 18.25 mg L-1, 173.8 mg L-1, and 668.6 mg L-1, respectively.					
36038978	6	18	theme	bacterium	1055:1063	arg1	fischeri					1072:1079	bioluminescent bacterium Vibrio fischeri	1040:1079	bioluminescent bacterium Vibrio fischeri	1040:1079	Micro-toxicity of antibiotics was performed using bioluminescent bacterium Vibrio fischeri and EC50 of CEF, TET and MNZ were found to be 18.25 mg L-1, 173.8 mg L-1, and 668.6 mg L-1, respectively.					
36038978	4	19	theme	hydrophobic	867:877	arg1	drugs					891:895	hydrophobic CEF and TET drugs	867:895	hydrophobic CEF and TET drugs	867:895	On the other hand, the hydrophilic MNZ was degraded faster compared to hydrophobic CEF and TET drugs.					
36038978	2	20	theme	UV	588:589	arg1	system					599:604	an UV reactor system	585:604	an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite)	585:646	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	2	21	theme	reactor	591:597	arg1	system					599:604	an UV reactor system	585:604	an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite)	585:646	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	8	22	theme	real-time	1607:1615	arg1	applications					1617:1628	real-time applications	1607:1628	real-time applications	1607:1628	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	6	23	theme	antibiotics	1008:1018	arg1	Micro-toxicity					990:1003	Micro-toxicity	990:1003	Micro-toxicity of antibiotics	990:1018	Micro-toxicity of antibiotics was performed using bioluminescent bacterium Vibrio fischeri and EC50 of CEF, TET and MNZ were found to be 18.25 mg L-1, 173.8 mg L-1, and 668.6 mg L-1, respectively.					
36038978	0	24	theme	multi-pollutant	124:138	arg1	conditions					140:149	single- and multi-pollutant conditions	112:149	Photocatalytic and adsorptive performance of polyvinyl alcohol/chitosan/TiO2 composite for antibiotics removal: single- and multi-pollutant conditions.	0:150	Photocatalytic and adsorptive performance of polyvinyl alcohol/chitosan/TiO2 composite for antibiotics removal: single- and multi-pollutant conditions.					
36038978	6	25	theme	Vibrio	1065:1070	arg1	fischeri					1072:1079	bioluminescent bacterium Vibrio fischeri	1040:1079	bioluminescent bacterium Vibrio fischeri	1040:1079	Micro-toxicity of antibiotics was performed using bioluminescent bacterium Vibrio fischeri and EC50 of CEF, TET and MNZ were found to be 18.25 mg L-1, 173.8 mg L-1, and 668.6 mg L-1, respectively.					
36038978	6	26	theme	TET	1098:1100	arg1	EC50					1085:1088	EC50	1085:1088	EC50 of CEF, TET and MNZ	1085:1108	Micro-toxicity of antibiotics was performed using bioluminescent bacterium Vibrio fischeri and EC50 of CEF, TET and MNZ were found to be 18.25 mg L-1, 173.8 mg L-1, and 668.6 mg L-1, respectively.					
36038978	2	27	theme	kinetic	669:675	arg1	models					677:682	kinetic models	669:682	kinetic models	669:682	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	8	28	theme	photocatalytic	1439:1452	arg1	cycles					1454:1459	three photocatalytic cycles	1433:1459	three photocatalytic cycles for all antibiotics at a minimal catalyst loading	1433:1509	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	1	29	theme	multi-compound	439:452	arg1	conditions					454:463	multi-compound conditions	439:463	multi-compound conditions	439:463	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	1	29	theme	multi-compound	439:452	arg1	composite					173:181	A polymer-TiO2 macro composite	152:181	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2)	152:201	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	7	30	theme	microtoxicity	1308:1320	arg1	analysis					1322:1329	the microtoxicity analysis	1304:1329	the microtoxicity analysis	1304:1329	However, the relative toxicity levels of PVA-CS-TiO2 and treated effluent were well with the limits as inferred from the microtoxicity analysis.					
36038978	2	31	theme	UV-C	612:615	arg1	L-1					630:632	0.3 g L-1	624:632	0.3 g L-1 of composite	624:645	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	2	31	theme	UV-C	612:615	arg1	power					617:621	32 W UV-C power	607:621	32 W UV-C power	607:621	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	6	32	theme	CEF	1093:1095	arg1	EC50					1085:1088	EC50	1085:1088	EC50 of CEF, TET and MNZ	1085:1108	Micro-toxicity of antibiotics was performed using bioluminescent bacterium Vibrio fischeri and EC50 of CEF, TET and MNZ were found to be 18.25 mg L-1, 173.8 mg L-1, and 668.6 mg L-1, respectively.					
36038978	3	33	theme	pseudo-second-order	717:735	arg1	kinetics					737:744	pseudo-second-order kinetics	717:744	pseudo-second-order kinetics	717:744	Antibiotics adsorption followed pseudo-second-order kinetics, and the order of adsorption was MNZ > TET > CEF.					
36038978	7	34	theme	relative	1200:1207	arg1	levels					1218:1223	the relative toxicity levels	1196:1223	the relative toxicity levels of PVA-CS-TiO2 and treated effluent	1196:1259	However, the relative toxicity levels of PVA-CS-TiO2 and treated effluent were well with the limits as inferred from the microtoxicity analysis.					
36038978	8	35	dep	exhibited	1374:1382	arg1	exhibit					1569:1575	exhibit	1569:1575	does not exhibit microtoxicity has a scope for real-time applications	1560:1628	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	8	35	dep	exhibited	1374:1382	arg1	retained					1519:1526	retained	1519:1526	easily retained using metallic tea strainer	1512:1554	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	2	36	theme	W	610:610	arg1	L-1					630:632	0.3 g L-1	624:632	0.3 g L-1 of composite	624:645	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	2	36	theme	W	610:610	arg1	power					617:621	32 W UV-C power	607:621	32 W UV-C power	607:621	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	0	37	theme	adsorptive	19:28	arg1	performance					30:40	adsorptive performance	19:40	adsorptive performance	19:40	Photocatalytic and adsorptive performance of polyvinyl alcohol/chitosan/TiO2 composite for antibiotics removal: single- and multi-pollutant conditions.					
36038978	4	38	theme	hydrophilic	819:829	arg1	MNZ					831:833	the hydrophilic MNZ	815:833	the hydrophilic MNZ	815:833	On the other hand, the hydrophilic MNZ was degraded faster compared to hydrophobic CEF and TET drugs.					
36038978	2	39	theme	antibiotics	512:522	arg1	removals					500:507	The photocatalytic and adsorptive removals	466:507	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite)	466:646	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	1	40	theme	chemical	223:230	arg1	precipitation					232:244	chemical precipitation	223:244	chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium	223:290	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	0	41	theme	polyvinyl	45:53	arg1	composite					77:85	polyvinyl alcohol/chitosan/TiO2 composite	45:85	polyvinyl alcohol/chitosan/TiO2 composite	45:85	Photocatalytic and adsorptive performance of polyvinyl alcohol/chitosan/TiO2 composite for antibiotics removal: single- and multi-pollutant conditions.					
36038978	1	42	dep	metronidazole	354:366	arg1	i.e.					348:351	i.e.	348:351	i.e.	348:351	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	5	43	theme	reactor	911:917	arg1	system					919:924	UV reactor system	908:924	UV reactor system	908:924	Moreover, UV reactor system exhibited antagonistic behavior under multi-compound condition.					
36038978	2	44	dep	L-1	560:562	arg1	mg					557:558	mg	557:558	mg	557:558	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	1	45	theme	model	329:333	arg1	antibiotics					335:345	three model antibiotics	323:345	three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET))	323:413	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	2	46	dep	antibiotics	512:522	arg1	concentrations					525:538	concentrations	525:538	concentrations of 0.1, 1 and 10 mg L-1	525:562	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	8	47	theme	synthesized	1338:1348	arg1	composite					1364:1372	synthesized biocompatible composite	1338:1372	synthesized biocompatible composite	1338:1372	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	1	48	from	precipitation	232:244	arg1	medium					285:290	alkali/solvent medium	270:290	alkali/solvent medium	270:290	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	8	49	theme	catalyst	1494:1501	arg1	loading					1503:1509	a minimal catalyst loading	1484:1509	a minimal catalyst loading	1484:1509	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	0	50	theme	composite	77:85	arg1	Photocatalytic					0:13	Photocatalytic	0:13	Photocatalytic	0:13	Photocatalytic and adsorptive performance of polyvinyl alcohol/chitosan/TiO2 composite for antibiotics removal: single- and multi-pollutant conditions.					
36038978	0	50	theme	composite	77:85	arg1	performance					30:40	adsorptive performance	19:40	adsorptive performance	19:40	Photocatalytic and adsorptive performance of polyvinyl alcohol/chitosan/TiO2 composite for antibiotics removal: single- and multi-pollutant conditions.					
36038978	1	51	theme	antibiotics	335:345	arg1	removal					312:318	the removal	308:318	the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET))	308:413	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	6	52	dep	mg	1147:1148	arg1	L-1					1150:1152	L-1	1150:1152	173.8 mg L-1	1141:1152	Micro-toxicity of antibiotics was performed using bioluminescent bacterium Vibrio fischeri and EC50 of CEF, TET and MNZ were found to be 18.25 mg L-1, 173.8 mg L-1, and 668.6 mg L-1, respectively.					
36038978	1	53	dep	PVA-CS-TiO2	190:200	arg1	i.e.					184:187	i.e.	184:187	i.e.	184:187	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	0	54	theme	alcohol/chitosan/TiO2	55:75	arg1	composite					77:85	polyvinyl alcohol/chitosan/TiO2 composite	45:85	polyvinyl alcohol/chitosan/TiO2 composite	45:85	Photocatalytic and adsorptive performance of polyvinyl alcohol/chitosan/TiO2 composite for antibiotics removal: single- and multi-pollutant conditions.					
36038978	3	55	theme	Antibiotics	685:695	arg1	adsorption					697:706	Antibiotics adsorption	685:706	Antibiotics adsorption	685:706	Antibiotics adsorption followed pseudo-second-order kinetics, and the order of adsorption was MNZ > TET > CEF.					
36038978	4	56	theme	other	803:807	arg1	hand					809:812	the other hand	799:812	the other hand	799:812	On the other hand, the hydrophilic MNZ was degraded faster compared to hydrophobic CEF and TET drugs.					
36038978	5	57	theme	antagonistic	936:947	arg1	behavior					949:956	antagonistic behavior	936:956	antagonistic behavior	936:956	Moreover, UV reactor system exhibited antagonistic behavior under multi-compound condition.					
36038978	2	58	theme	g	628:628	arg1	L-1					630:632	0.3 g L-1	624:632	0.3 g L-1 of composite	624:645	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	2	58	theme	g	628:628	arg1	power					617:621	32 W UV-C power	607:621	32 W UV-C power	607:621	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	3	59	theme	TET	785:787	arg1	order					755:759	the order	751:759	the order of adsorption	751:773	Antibiotics adsorption followed pseudo-second-order kinetics, and the order of adsorption was MNZ > TET > CEF.					
36038978	3	59	theme	TET	785:787	arg1	CEF					791:793	MNZ > TET > CEF	779:793	MNZ > TET > CEF	779:793	Antibiotics adsorption followed pseudo-second-order kinetics, and the order of adsorption was MNZ > TET > CEF.					
36038978	5	60	theme	UV	908:909	arg1	system					919:924	UV reactor system	908:924	UV reactor system	908:924	Moreover, UV reactor system exhibited antagonistic behavior under multi-compound condition.					
36038978	8	61	theme	biocompatible	1350:1362	arg1	composite					1364:1372	synthesized biocompatible composite	1338:1372	synthesized biocompatible composite	1338:1372	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	7	62	theme	PVA-CS-TiO2	1228:1238	arg1	levels					1218:1223	the relative toxicity levels	1196:1223	the relative toxicity levels of PVA-CS-TiO2 and treated effluent	1196:1259	However, the relative toxicity levels of PVA-CS-TiO2 and treated effluent were well with the limits as inferred from the microtoxicity analysis.					
36038978	1	63	theme	polymer-TiO2	154:165	arg1	PVA-CS-TiO2					190:200	PVA-CS-TiO2	190:200	PVA-CS-TiO2	190:200	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	1	63	theme	polymer-TiO2	154:165	arg1	compound					426:433	single compound	419:433	single compound	419:433	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	1	63	theme	polymer-TiO2	154:165	arg1	conditions					454:463	multi-compound conditions	439:463	multi-compound conditions	439:463	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	1	63	theme	polymer-TiO2	154:165	arg1	composite					173:181	A polymer-TiO2 macro composite	152:181	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2)	152:201	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	3	64	theme	>	789:789	arg1	order					755:759	the order	751:759	the order of adsorption	751:773	Antibiotics adsorption followed pseudo-second-order kinetics, and the order of adsorption was MNZ > TET > CEF.					
36038978	3	64	theme	>	789:789	arg1	CEF					791:793	MNZ > TET > CEF	779:793	MNZ > TET > CEF	779:793	Antibiotics adsorption followed pseudo-second-order kinetics, and the order of adsorption was MNZ > TET > CEF.					
36038978	1	65	theme	PVA-CS-TiO2	249:259	arg1	blend					261:265	PVA-CS-TiO2 blend	249:265	PVA-CS-TiO2 blend	249:265	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	5	66	theme	multi-compound	964:977	arg1	condition					979:987	multi-compound condition	964:987	multi-compound condition	964:987	Moreover, UV reactor system exhibited antagonistic behavior under multi-compound condition.					
36038978	2	67	theme	adsorptive	489:498	arg1	removals					500:507	The photocatalytic and adsorptive removals	466:507	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite)	466:646	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	8	68	dep	microtoxicity	1577:1589	arg1	has					1591:1593	has	1591:1593	has a scope for real-time applications	1591:1628	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	1	69	theme	macro	167:171	arg1	PVA-CS-TiO2					190:200	PVA-CS-TiO2	190:200	PVA-CS-TiO2	190:200	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	1	69	theme	macro	167:171	arg1	compound					426:433	single compound	419:433	single compound	419:433	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	1	69	theme	macro	167:171	arg1	conditions					454:463	multi-compound conditions	439:463	multi-compound conditions	439:463	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	1	69	theme	macro	167:171	arg1	composite					173:181	A polymer-TiO2 macro composite	152:181	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2)	152:201	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	3	70	theme	MNZ	779:781	arg1	order					755:759	the order	751:759	the order of adsorption	751:773	Antibiotics adsorption followed pseudo-second-order kinetics, and the order of adsorption was MNZ > TET > CEF.					
36038978	3	70	theme	MNZ	779:781	arg1	CEF					791:793	MNZ > TET > CEF	779:793	MNZ > TET > CEF	779:793	Antibiotics adsorption followed pseudo-second-order kinetics, and the order of adsorption was MNZ > TET > CEF.					
36038978	1	71	theme	blend	261:265	arg1	precipitation					232:244	chemical precipitation	223:244	chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium	223:290	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36038978	0	72	theme	antibiotics	91:101	arg1	removal					103:109	antibiotics removal	91:109	antibiotics removal	91:109	Photocatalytic and adsorptive performance of polyvinyl alcohol/chitosan/TiO2 composite for antibiotics removal: single- and multi-pollutant conditions.					
36038978	6	73	theme	bioluminescent	1040:1053	arg1	fischeri					1072:1079	bioluminescent bacterium Vibrio fischeri	1040:1079	bioluminescent bacterium Vibrio fischeri	1040:1079	Micro-toxicity of antibiotics was performed using bioluminescent bacterium Vibrio fischeri and EC50 of CEF, TET and MNZ were found to be 18.25 mg L-1, 173.8 mg L-1, and 668.6 mg L-1, respectively.					
36038978	3	74	theme	>	783:783	arg1	order					755:759	the order	751:759	the order of adsorption	751:773	Antibiotics adsorption followed pseudo-second-order kinetics, and the order of adsorption was MNZ > TET > CEF.					
36038978	3	74	theme	>	783:783	arg1	CEF					791:793	MNZ > TET > CEF	779:793	MNZ > TET > CEF	779:793	Antibiotics adsorption followed pseudo-second-order kinetics, and the order of adsorption was MNZ > TET > CEF.					
36038978	8	75	theme	minimal	1486:1492	arg1	loading					1503:1509	a minimal catalyst loading	1484:1509	a minimal catalyst loading	1484:1509	Thus, synthesized biocompatible composite exhibited structural stability, consistent performance for three photocatalytic cycles for all antibiotics at a minimal catalyst loading, easily retained using metallic tea strainer and does not exhibit microtoxicity has a scope for real-time applications.					
36038978	4	76	theme	CEF	879:881	arg1	drugs					891:895	hydrophobic CEF and TET drugs	867:895	hydrophobic CEF and TET drugs	867:895	On the other hand, the hydrophilic MNZ was degraded faster compared to hydrophobic CEF and TET drugs.					
36038978	7	77	theme	treated	1244:1250	arg1	effluent					1252:1259	treated effluent	1244:1259	treated effluent	1244:1259	However, the relative toxicity levels of PVA-CS-TiO2 and treated effluent were well with the limits as inferred from the microtoxicity analysis.					
36038978	0	78	dep	Photocatalytic	0:13	arg1	conditions					140:149	single- and multi-pollutant conditions	112:149	Photocatalytic and adsorptive performance of polyvinyl alcohol/chitosan/TiO2 composite for antibiotics removal: single- and multi-pollutant conditions.	0:150	Photocatalytic and adsorptive performance of polyvinyl alcohol/chitosan/TiO2 composite for antibiotics removal: single- and multi-pollutant conditions.					
36038978	6	79	dep	mg	1133:1134	arg1	L-1					1136:1138	L-1	1136:1138	18.25 mg L-1	1127:1138	Micro-toxicity of antibiotics was performed using bioluminescent bacterium Vibrio fischeri and EC50 of CEF, TET and MNZ were found to be 18.25 mg L-1, 173.8 mg L-1, and 668.6 mg L-1, respectively.					
36038978	2	80	dep	system	599:604	arg1	L-1					630:632	0.3 g L-1	624:632	0.3 g L-1 of composite	624:645	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	2	80	dep	system	599:604	arg1	power					617:621	32 W UV-C power	607:621	32 W UV-C power	607:621	The photocatalytic and adsorptive removals of antibiotics (concentrations of 0.1, 1 and 10 mg L-1) by the composite in an UV reactor system (32 W UV-C power, 0.3 g L-1 of composite) was assessed through kinetic models.					
36038978	1	81	theme	alkali/solvent	270:283	arg1	medium					285:290	alkali/solvent medium	270:290	alkali/solvent medium	270:290	A polymer-TiO2 macro composite (i.e., PVA-CS-TiO2) was synthesized via chemical precipitation of PVA-CS-TiO2 blend in alkali/solvent medium and applied for the removal of three model antibiotics (i.e., metronidazole (MNZ), ceftiofur (CEF) and tetracycline (TET)), as single compound and multi-compound conditions.					
36174859	7	0	theme	Ow	1155:1156	arg1	tracheids					1158:1166	Ow tracheids	1155:1166	Ow tracheids	1155:1166	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	5	1	theme	pretreatment	862:873	arg1	wall					847:850	secondary wall	837:850	secondary wall of Ow and pretreatment	837:873	Raman imaging further revealed that both lignin and carbohydrates were dissolved simultaneously within the middle lamella and secondary wall of Ow and pretreatment has little effects on Cw tracheids wall.					
36174859	7	2	theme	middle	1100:1105	arg1	%					1121:1121	52.3 %	1116:1121	52.3 %	1116:1121	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	7	2	theme	middle	1100:1105	arg1	lamella					1107:1113	cell corner middle lamella	1088:1113	cell corner middle lamella (52.3 %)	1088:1122	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	5	3	theme	middle	818:823	arg1	lamella					825:831	the middle lamella	814:831	the middle lamella	814:831	Raman imaging further revealed that both lignin and carbohydrates were dissolved simultaneously within the middle lamella and secondary wall of Ow and pretreatment has little effects on Cw tracheids wall.					
36174859	5	4	theme	little	879:884	arg1	effects					886:892	little effects	879:892	little effects	879:892	Raman imaging further revealed that both lignin and carbohydrates were dissolved simultaneously within the middle lamella and secondary wall of Ow and pretreatment has little effects on Cw tracheids wall.					
36174859	6	5	theme	key	978:980	arg1	composition					958:968	lignin composition	951:968	lignin composition	951:968	Moreover, it was demonstrated that lignin composition was the key factor to affect the composition dissolution.					
36174859	6	5	theme	key	978:980	arg1	factor					982:987	the key factor to affect the composition dissolution	974:1025	the key factor to affect the composition dissolution	974:1025	Moreover, it was demonstrated that lignin composition was the key factor to affect the composition dissolution.					
36174859	5	6	theme	Cw	897:898	arg1	wall					910:913	Cw tracheids wall	897:913	Cw tracheids wall	897:913	Raman imaging further revealed that both lignin and carbohydrates were dissolved simultaneously within the middle lamella and secondary wall of Ow and pretreatment has little effects on Cw tracheids wall.					
36174859	4	7	theme	ionic	490:494	arg1	treatment					504:512	The ionic liqiuds treatment	486:512	The ionic liqiuds treatment	486:512	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
36174859	4	8	theme	lignin	660:665	arg1	removal					685:691	the higher lignin and carbohydrates removal	649:691	the higher lignin and carbohydrates removal for Ow tracheids	649:708	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
36174859	1	9	theme	natural	242:248	arg1	lignin					150:155	lignin	150:155	lignin	150:155	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	1	9	theme	natural	242:248	arg1	recalcitrance					258:270	the natural biomass recalcitrance	238:270	the natural biomass recalcitrance	238:270	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	7	10	theme	cell	1088:1091	arg1	%					1121:1121	52.3 %	1116:1121	52.3 %	1116:1121	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	7	10	theme	cell	1088:1091	arg1	lamella					1107:1113	cell corner middle lamella	1088:1113	cell corner middle lamella (52.3 %)	1088:1122	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	6	11	theme	lignin	951:956	arg1	composition					958:968	lignin composition	951:968	lignin composition	951:968	Moreover, it was demonstrated that lignin composition was the key factor to affect the composition dissolution.					
36174859	6	11	theme	lignin	951:956	arg1	factor					982:987	the key factor to affect the composition dissolution	974:1025	the key factor to affect the composition dissolution	974:1025	Moreover, it was demonstrated that lignin composition was the key factor to affect the composition dissolution.					
36174859	1	12	theme	biomass	250:256	arg1	lignin					150:155	lignin	150:155	lignin	150:155	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	1	12	theme	biomass	250:256	arg1	recalcitrance					258:270	the natural biomass recalcitrance	238:270	the natural biomass recalcitrance	238:270	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	7	13	theme	corner	1093:1098	arg1	%					1121:1121	52.3 %	1116:1121	52.3 %	1116:1121	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	7	13	theme	corner	1093:1098	arg1	lamella					1107:1113	cell corner middle lamella	1088:1113	cell corner middle lamella (52.3 %)	1088:1122	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	4	14	theme	lignified	562:570	arg1	tracheids					575:583	highly lignified Cw tracheids	555:583	highly lignified Cw tracheids	555:583	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
36174859	5	15	theme	Raman	711:715	arg1	imaging					717:723	Raman imaging	711:723	Raman imaging	711:723	Raman imaging further revealed that both lignin and carbohydrates were dissolved simultaneously within the middle lamella and secondary wall of Ow and pretreatment has little effects on Cw tracheids wall.					
36174859	9	16	theme	wall	1427:1430	arg1	behavior					1383:1390	the dissolution behavior	1367:1390	the dissolution behavior of carbohydrate dominant tracheids wall	1367:1430	The findings confirmed the important role of lignin compostion in the dissolution behavior of carbohydrate dominant tracheids wall.					
36174859	2	17	theme	Pinus	396:400	arg1	Zucc					411:414	Pinus bungeana Zucc	396:414	Pinus bungeana Zucc	396:414	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	2	18	theme	Substantial	273:283	arg1	differences					285:295	Substantial differences	273:295	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.	273:415	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	5	19	theme	tracheids	900:908	arg1	wall					910:913	Cw tracheids wall	897:913	Cw tracheids wall	897:913	Raman imaging further revealed that both lignin and carbohydrates were dissolved simultaneously within the middle lamella and secondary wall of Ow and pretreatment has little effects on Cw tracheids wall.					
36174859	4	20	theme	spectra	622:628	arg1	analysis					630:637	dynamic Raman spectra analysis	608:637	dynamic Raman spectra analysis	608:637	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
36174859	9	21	theme	compostion	1353:1362	arg1	role					1338:1341	the important role	1324:1341	the important role of lignin compostion in the dissolution behavior of carbohydrate dominant tracheids wall	1324:1430	The findings confirmed the important role of lignin compostion in the dissolution behavior of carbohydrate dominant tracheids wall.					
36174859	2	22	theme	Cw	379:380	arg1	trachieds					383:391	opposite (Ow) and compression wood (Cw) trachieds	343:391	trachieds	383:391	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	0	23	from	dissolution	37:47	arg1	wood					79:82	reaction wood	70:82	reaction wood	70:82	In-situ visualizing selective lignin dissolution of tracheids wall in reaction wood.					
36174859	1	24	with	macromolecule	111:123	arg1	structure					139:147	aromatic structure	130:147	aromatic structure	130:147	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	2	25	from	Ow	353:354	arg1	Zucc					411:414	Pinus bungeana Zucc	396:414	Pinus bungeana Zucc	396:414	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	2	26	theme	lignin	314:319	arg1	topochemistry					321:333	the cell wall lignin topochemistry	300:333	the cell wall lignin topochemistry	300:333	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	5	27	theme	Ow	855:856	arg1	wall					847:850	secondary wall	837:850	secondary wall of Ow and pretreatment	837:873	Raman imaging further revealed that both lignin and carbohydrates were dissolved simultaneously within the middle lamella and secondary wall of Ow and pretreatment has little effects on Cw tracheids wall.					
36174859	4	28	theme	obvious	529:535	arg1	swelling					542:549	a more obvious wall swelling	522:549	a more obvious wall swelling for highly lignified Cw tracheids	522:583	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
36174859	9	29	theme	lignin	1346:1351	arg1	compostion					1353:1362	lignin compostion	1346:1362	lignin compostion	1346:1362	The findings confirmed the important role of lignin compostion in the dissolution behavior of carbohydrate dominant tracheids wall.					
36174859	2	30	theme	wall	309:312	arg1	topochemistry					321:333	the cell wall lignin topochemistry	300:333	the cell wall lignin topochemistry	300:333	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	2	31	theme	wood	373:376	arg1	trachieds					383:391	opposite (Ow) and compression wood (Cw) trachieds	343:391	trachieds	383:391	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	0	32	theme	lignin	30:35	arg1	dissolution					37:47	selective lignin dissolution	20:47	selective lignin dissolution of tracheids wall in reaction wood	20:82	In-situ visualizing selective lignin dissolution of tracheids wall in reaction wood.					
36174859	7	33	theme	lignin	1043:1048	arg1	G-units					1050:1056	lignin G-units	1043:1056	lignin G-units	1043:1056	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	2	34	theme	cell	304:307	arg1	topochemistry					321:333	the cell wall lignin topochemistry	300:333	the cell wall lignin topochemistry	300:333	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	9	35	theme	dissolution	1371:1381	arg1	behavior					1383:1390	the dissolution behavior	1367:1390	the dissolution behavior of carbohydrate dominant tracheids wall	1367:1430	The findings confirmed the important role of lignin compostion in the dissolution behavior of carbohydrate dominant tracheids wall.					
36174859	2	36	theme	bungeana	402:409	arg1	Zucc					411:414	Pinus bungeana Zucc	396:414	Pinus bungeana Zucc	396:414	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	0	37	theme	selective	20:28	arg1	dissolution					37:47	selective lignin dissolution	20:47	selective lignin dissolution of tracheids wall in reaction wood	20:82	In-situ visualizing selective lignin dissolution of tracheids wall in reaction wood.					
36174859	1	38	theme	biological	100:109	arg1	lignin					150:155	lignin	150:155	lignin	150:155	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	1	38	theme	biological	100:109	arg1	macromolecule					111:123	a renewable biological macromolecule	88:123	a renewable biological macromolecule with aromatic structure	88:147	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	4	39	theme	Raman	616:620	arg1	analysis					630:637	dynamic Raman spectra analysis	608:637	dynamic Raman spectra analysis	608:637	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
36174859	1	40	theme	cell	199:202	arg1	integrity					209:217	cell wall integrity	199:217	cell wall integrity	199:217	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	7	41	theme	tracheids	1158:1166	arg1	%					1121:1121	52.3 %	1116:1121	52.3 %	1116:1121	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	7	41	theme	tracheids	1158:1166	arg1	wall					1138:1141	secondary wall	1128:1141	secondary wall (62.0 %)	1128:1150	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	7	41	theme	tracheids	1158:1166	arg1	%					1149:1149	62.0 %	1144:1149	62.0 %	1144:1149	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	7	41	theme	tracheids	1158:1166	arg1	lamella					1107:1113	cell corner middle lamella	1088:1113	cell corner middle lamella (52.3 %)	1088:1122	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	3	42	theme	room	468:471	arg1	temperature					473:483	room temperature	468:483	room temperature	468:483	were visualized during [Emim][OAc] pretreatment at room temperature.					
36174859	9	43	from	role	1338:1341	arg1	behavior					1383:1390	the dissolution behavior	1367:1390	the dissolution behavior of carbohydrate dominant tracheids wall	1367:1430	The findings confirmed the important role of lignin compostion in the dissolution behavior of carbohydrate dominant tracheids wall.					
36174859	4	44	theme	dynamic	608:614	arg1	analysis					630:637	dynamic Raman spectra analysis	608:637	dynamic Raman spectra analysis	608:637	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
36174859	2	45	from	differences	285:295	arg1	topochemistry					321:333	the cell wall lignin topochemistry	300:333	the cell wall lignin topochemistry	300:333	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	1	46	theme	wall	204:207	arg1	integrity					209:217	cell wall integrity	199:217	cell wall integrity	199:217	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	4	47	theme	higher	653:658	arg1	removal					685:691	the higher lignin and carbohydrates removal	649:691	the higher lignin and carbohydrates removal for Ow tracheids	649:708	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
36174859	9	48	theme	carbohydrate	1395:1406	arg1	wall					1427:1430	carbohydrate dominant tracheids wall	1395:1430	carbohydrate dominant tracheids wall	1395:1430	The findings confirmed the important role of lignin compostion in the dissolution behavior of carbohydrate dominant tracheids wall.					
36174859	9	49	theme	tracheids	1417:1425	arg1	wall					1427:1430	carbohydrate dominant tracheids wall	1395:1430	carbohydrate dominant tracheids wall	1395:1430	The findings confirmed the important role of lignin compostion in the dissolution behavior of carbohydrate dominant tracheids wall.					
36174859	4	50	theme	wall	537:540	arg1	swelling					542:549	a more obvious wall swelling	522:549	a more obvious wall swelling for highly lignified Cw tracheids	522:583	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
36174859	5	51	theme	secondary	837:845	arg1	wall					847:850	secondary wall	837:850	secondary wall of Ow and pretreatment	837:873	Raman imaging further revealed that both lignin and carbohydrates were dissolved simultaneously within the middle lamella and secondary wall of Ow and pretreatment has little effects on Cw tracheids wall.					
36174859	0	52	theme	wall	62:65	arg1	dissolution					37:47	selective lignin dissolution	20:47	selective lignin dissolution of tracheids wall in reaction wood	20:82	In-situ visualizing selective lignin dissolution of tracheids wall in reaction wood.					
36174859	4	53	theme	liqiuds	496:502	arg1	treatment					504:512	The ionic liqiuds treatment	486:512	The ionic liqiuds treatment	486:512	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
36174859	9	54	theme	dominant	1408:1415	arg1	wall					1427:1430	carbohydrate dominant tracheids wall	1395:1430	carbohydrate dominant tracheids wall	1395:1430	The findings confirmed the important role of lignin compostion in the dissolution behavior of carbohydrate dominant tracheids wall.					
36174859	3	55	theme	[OAc	446:449	arg1	pretreatment					452:463	[Emim][OAc] pretreatment	440:463	[Emim][OAc] pretreatment	440:463	were visualized during [Emim][OAc] pretreatment at room temperature.					
36174859	0	56	theme	tracheids	52:60	arg1	wall					62:65	tracheids wall	52:65	tracheids wall	52:65	In-situ visualizing selective lignin dissolution of tracheids wall in reaction wood.					
36174859	8	57	dep	cellulose	1273:1281	arg1	to					1285:1286	to	1285:1286	to	1285:1286	When cotton fiber, as a reference was treated under the same conditions, lattice conversion moving from cellulose I to II occurred.					
36174859	2	58	theme	opposite	343:350	arg1	Ow					353:354	opposite (Ow) and compression wood (Cw) trachieds	343:391	Ow	353:354	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	7	59	attach	removed	1075:1081	arg2	G-units					1050:1056	lignin G-units	1043:1056	lignin G-units	1043:1056	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	7	59	attach	removed	1075:1081	arg1	%					1121:1121	52.3 %	1116:1121	52.3 %	1116:1121	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	7	59	attach	removed	1075:1081	arg1	wall					1138:1141	secondary wall	1128:1141	secondary wall (62.0 %)	1128:1150	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	7	59	attach	removed	1075:1081	arg1	%					1149:1149	62.0 %	1144:1149	62.0 %	1144:1149	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	7	59	attach	removed	1075:1081	arg1	lamella					1107:1113	cell corner middle lamella	1088:1113	cell corner middle lamella (52.3 %)	1088:1122	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	1	60	theme	aromatic	130:137	arg1	structure					139:147	aromatic structure	130:147	aromatic structure	130:147	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	1	61	theme	renewable	90:98	arg1	lignin					150:155	lignin	150:155	lignin	150:155	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	1	61	theme	renewable	90:98	arg1	macromolecule					111:123	a renewable biological macromolecule	88:123	a renewable biological macromolecule with aromatic structure	88:147	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	8	62	theme	lattice	1242:1248	arg1	conversion					1250:1259	lattice conversion	1242:1259	lattice conversion moving from cellulose I to II	1242:1289	When cotton fiber, as a reference was treated under the same conditions, lattice conversion moving from cellulose I to II occurred.					
36174859	8	63	theme	same	1225:1228	arg1	conditions					1230:1239	the same conditions	1221:1239	the same conditions	1221:1239	When cotton fiber, as a reference was treated under the same conditions, lattice conversion moving from cellulose I to II occurred.					
36174859	4	64	theme	Ow	697:698	arg1	tracheids					700:708	Ow tracheids	697:708	Ow tracheids	697:708	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
36174859	0	65	theme	reaction	70:77	arg1	wood					79:82	reaction wood	70:82	reaction wood	70:82	In-situ visualizing selective lignin dissolution of tracheids wall in reaction wood.					
36174859	6	66	theme	composition	1003:1013	arg1	dissolution					1015:1025	the composition dissolution	999:1025	the composition dissolution	999:1025	Moreover, it was demonstrated that lignin composition was the key factor to affect the composition dissolution.					
36174859	2	67	from	trachieds	383:391	arg1	Zucc					411:414	Pinus bungeana Zucc	396:414	Pinus bungeana Zucc	396:414	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	2	68	theme	compression	361:371	arg1	trachieds					383:391	opposite (Ow) and compression wood (Cw) trachieds	343:391	trachieds	383:391	Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc.					
36174859	1	69	theme	matrix	170:175	arg1	substance					177:185	matrix substance	170:185	matrix substance	170:185	As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance.					
36174859	4	70	theme	Cw	572:573	arg1	tracheids					575:583	highly lignified Cw tracheids	555:583	highly lignified Cw tracheids	555:583	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
36174859	8	71	theme	cotton	1174:1179	arg1	fiber					1181:1185	cotton fiber	1174:1185	cotton fiber	1174:1185	When cotton fiber, as a reference was treated under the same conditions, lattice conversion moving from cellulose I to II occurred.					
36174859	3	72	dep	[OAc	446:449	arg1	[Emim					440:444	[Emim	440:444	[Emim	440:444	were visualized during [Emim][OAc] pretreatment at room temperature.					
36174859	5	73	contain	has	875:877	arg2	effects					886:892	little effects	879:892	little effects	879:892	Raman imaging further revealed that both lignin and carbohydrates were dissolved simultaneously within the middle lamella and secondary wall of Ow and pretreatment has little effects on Cw tracheids wall.					
36174859	5	73	contain	has	875:877	arg1	wall					847:850	secondary wall	837:850	secondary wall of Ow and pretreatment	837:873	Raman imaging further revealed that both lignin and carbohydrates were dissolved simultaneously within the middle lamella and secondary wall of Ow and pretreatment has little effects on Cw tracheids wall.					
36174859	7	74	theme	secondary	1128:1136	arg1	wall					1138:1141	secondary wall	1128:1141	secondary wall (62.0 %)	1128:1150	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	7	74	theme	secondary	1128:1136	arg1	%					1149:1149	62.0 %	1144:1149	62.0 %	1144:1149	In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids.					
36174859	9	75	theme	important	1328:1336	arg1	role					1338:1341	the important role	1324:1341	the important role of lignin compostion in the dissolution behavior of carbohydrate dominant tracheids wall	1324:1430	The findings confirmed the important role of lignin compostion in the dissolution behavior of carbohydrate dominant tracheids wall.					
36174859	4	76	theme	carbohydrates	671:683	arg1	removal					685:691	the higher lignin and carbohydrates removal	649:691	the higher lignin and carbohydrates removal for Ow tracheids	649:708	The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids.					
37173019	0	0	theme	resistant	83:91	arg1	starch					93:98	resistant starch	83:98	resistant starch	83:98	Gut microbiota-metabolic axis insight into the hyperlipidemic effect of lotus seed resistant starch in hyperlipidemic mice.					
37173019	1	1	theme	hyperlipidemic	296:309	arg1	mice					311:314	hyperlipidemic mice	296:314	hyperlipidemic mice	296:314	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	1	2	theme	LRS	244:246	arg1	MLRS					264:267	low-, medium and high-dose LRS, named as LLRS, MLRS	217:267	low-, medium and high-dose LRS, named as LLRS, MLRS	217:267	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	3	3	theme	deoxycholic	665:675	arg1	acid					677:680	deoxycholic acid	665:680	deoxycholic acid	665:680	Moreover, supplementation of LRS promoted cholic acid (CA) production and inhibited deoxycholic acid compared to MC group.					
37173019	4	4	theme	Oleic	841:845	arg1	acid					847:850	Oleic acid	841:850	Oleic acid	841:850	Among, LLRS promoted formic acid, MLRS inhibited 20-Carboxy-leukotriene B4, while HLRS promoted 3, 4-Methyleneazelaic acid and inhibited Oleic acid and Malic acid.					
37173019	2	5	theme	LRS	453:455	arg1	groups					457:462	LRS groups	453:462	LRS groups	453:462	Allobaculum was significantly decreased in LRS groups compared to MC group, while MLRS promoted the abundance of norank_f_Muribaculaceae and norank_f_Erysipelotrichaceae.					
37173019	1	6	theme	different	169:177	arg1	doses					179:183	different doses	169:183	different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively)	169:291	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	1	7	dep	group	398:402	arg1	MC					405:406	MC	405:406	MC	405:406	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	4	8	theme	Malic	856:860	arg1	acid					862:865	Malic acid	856:865	Malic acid	856:865	Among, LLRS promoted formic acid, MLRS inhibited 20-Carboxy-leukotriene B4, while HLRS promoted 3, 4-Methyleneazelaic acid and inhibited Oleic acid and Malic acid.					
37173019	5	9	theme	cholesterol	933:943	arg1	catabolism					945:954	this promoted cholesterol catabolism	919:954	this promoted cholesterol catabolism	919:954	Finally, MLRS regulate microbiota composition, and this promoted cholesterol catabolism to form CA, which inhibited serum lipid index by gut microbiota-metabolic axis.					
37173019	1	10	theme	gut	322:324	arg1	axis					347:350	gut microbiota-metabolic axis	322:350	gut microbiota-metabolic axis	322:350	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	2	11	theme	norank_f_Erysipelotrichaceae	551:578	arg1	abundance					510:518	the abundance	506:518	the abundance of norank_f_Muribaculaceae and norank_f_Erysipelotrichaceae	506:578	Allobaculum was significantly decreased in LRS groups compared to MC group, while MLRS promoted the abundance of norank_f_Muribaculaceae and norank_f_Erysipelotrichaceae.					
37173019	2	12	theme	MC	476:477	arg1	group					479:483	MC group	476:483	MC group	476:483	Allobaculum was significantly decreased in LRS groups compared to MC group, while MLRS promoted the abundance of norank_f_Muribaculaceae and norank_f_Erysipelotrichaceae.					
37173019	1	13	theme	microbiota-metabolic	326:345	arg1	axis					347:350	gut microbiota-metabolic axis	322:350	gut microbiota-metabolic axis	322:350	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	1	14	from	effect	159:164	arg1	mice					311:314	hyperlipidemic mice	296:314	hyperlipidemic mice	296:314	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	1	15	theme	doses	179:183	arg1	effect					159:164	the hyperlipidemic effect	140:164	the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice	140:314	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	0	16	theme	hyperlipidemic	103:116	arg1	mice					118:121	hyperlipidemic mice	103:121	hyperlipidemic mice	103:121	Gut microbiota-metabolic axis insight into the hyperlipidemic effect of lotus seed resistant starch in hyperlipidemic mice.					
37173019	3	17	theme	MC	694:695	arg1	group					697:701	MC group	694:701	MC group	694:701	Moreover, supplementation of LRS promoted cholic acid (CA) production and inhibited deoxycholic acid compared to MC group.					
37173019	4	18	theme	4-Methyleneazelaic	803:820	arg1	acid					822:825	3, 4-Methyleneazelaic acid	800:825	acid	822:825	Among, LLRS promoted formic acid, MLRS inhibited 20-Carboxy-leukotriene B4, while HLRS promoted 3, 4-Methyleneazelaic acid and inhibited Oleic acid and Malic acid.					
37173019	0	19	theme	microbiota-metabolic	4:23	arg1	insight					30:36	Gut microbiota-metabolic axis insight	0:36	Gut microbiota-metabolic axis insight into the hyperlipidemic effect of lotus	0:76	Gut microbiota-metabolic axis insight into the hyperlipidemic effect of lotus seed resistant starch in hyperlipidemic mice.					
37173019	1	20	theme	lotus	188:192	arg1	starch					209:214	lotus seed resistant starch	188:214	lotus seed resistant starch	188:214	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	0	21	theme	Gut	0:2	arg1	insight					30:36	Gut microbiota-metabolic axis insight	0:36	Gut microbiota-metabolic axis insight into the hyperlipidemic effect of lotus	0:76	Gut microbiota-metabolic axis insight into the hyperlipidemic effect of lotus seed resistant starch in hyperlipidemic mice.					
37173019	1	22	theme	seed	194:197	arg1	starch					209:214	lotus seed resistant starch	188:214	lotus seed resistant starch	188:214	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	4	23	theme	formic	725:730	arg1	acid					732:735	formic acid	725:735	formic acid	725:735	Among, LLRS promoted formic acid, MLRS inhibited 20-Carboxy-leukotriene B4, while HLRS promoted 3, 4-Methyleneazelaic acid and inhibited Oleic acid and Malic acid.					
37173019	1	24	theme	resistant	199:207	arg1	starch					209:214	lotus seed resistant starch	188:214	lotus seed resistant starch	188:214	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	0	25	theme	axis	25:28	arg1	insight					30:36	Gut microbiota-metabolic axis insight	0:36	Gut microbiota-metabolic axis insight into the hyperlipidemic effect of lotus	0:76	Gut microbiota-metabolic axis insight into the hyperlipidemic effect of lotus seed resistant starch in hyperlipidemic mice.					
37173019	6	26	theme	best	1127:1130	arg1	role					1132:1135	the best role	1123:1135	the best role	1123:1135	In conclusion, MLRS can promote CA and inhibit medium chain fatty acids, so as to play the best role in lowering blood lipids in hyperlipidemia mice.					
37173019	5	27	theme	promoted	924:931	arg1	catabolism					945:954	this promoted cholesterol catabolism	919:954	this promoted cholesterol catabolism	919:954	Finally, MLRS regulate microbiota composition, and this promoted cholesterol catabolism to form CA, which inhibited serum lipid index by gut microbiota-metabolic axis.					
37173019	1	28	theme	starch	209:214	arg1	doses					179:183	different doses	169:183	different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively)	169:291	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	6	29	theme	chain	1090:1094	arg1	acids					1102:1106	medium chain fatty acids	1083:1106	medium chain fatty acids	1083:1106	In conclusion, MLRS can promote CA and inhibit medium chain fatty acids, so as to play the best role in lowering blood lipids in hyperlipidemia mice.					
37173019	1	30	theme	high-fat	364:371	arg1	mice					378:381	high-fat diet mice	364:381	high-fat diet mice (model control group, MC)	364:407	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	1	30	theme	high-fat	364:371	arg1	group					398:402	model control group	384:402	model control group	384:402	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	5	31	theme	serum	984:988	arg1	index					996:1000	serum lipid index	984:1000	serum lipid index	984:1000	Finally, MLRS regulate microbiota composition, and this promoted cholesterol catabolism to form CA, which inhibited serum lipid index by gut microbiota-metabolic axis.					
37173019	1	32	theme	diet	373:376	arg1	mice					378:381	high-fat diet mice	364:381	high-fat diet mice (model control group, MC)	364:407	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	1	32	theme	diet	373:376	arg1	group					398:402	model control group	384:402	model control group	384:402	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	5	33	theme	lipid	990:994	arg1	index					996:1000	serum lipid index	984:1000	serum lipid index	984:1000	Finally, MLRS regulate microbiota composition, and this promoted cholesterol catabolism to form CA, which inhibited serum lipid index by gut microbiota-metabolic axis.					
37173019	1	34	theme	low-	217:220	arg1	MLRS					264:267	low-, medium and high-dose LRS, named as LLRS, MLRS	217:267	low-, medium and high-dose LRS, named as LLRS, MLRS	217:267	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	3	35	theme	acid	630:633	arg1	production					640:649	cholic acid (CA) production	623:649	cholic acid (CA) production	623:649	Moreover, supplementation of LRS promoted cholic acid (CA) production and inhibited deoxycholic acid compared to MC group.					
37173019	1	36	dep	doses	179:183	arg1	MLRS					264:267	low-, medium and high-dose LRS, named as LLRS, MLRS	217:267	low-, medium and high-dose LRS, named as LLRS, MLRS	217:267	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	1	36	dep	doses	179:183	arg1	HLRS					273:276	HLRS	273:276	HLRS	273:276	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	6	37	theme	medium	1083:1088	arg1	acids					1102:1106	medium chain fatty acids	1083:1106	medium chain fatty acids	1083:1106	In conclusion, MLRS can promote CA and inhibit medium chain fatty acids, so as to play the best role in lowering blood lipids in hyperlipidemia mice.					
37173019	6	38	theme	fatty	1096:1100	arg1	acids					1102:1106	medium chain fatty acids	1083:1106	medium chain fatty acids	1083:1106	In conclusion, MLRS can promote CA and inhibit medium chain fatty acids, so as to play the best role in lowering blood lipids in hyperlipidemia mice.					
37173019	0	39	theme	hyperlipidemic	47:60	arg1	effect					62:67	the hyperlipidemic effect	43:67	the hyperlipidemic effect of lotus	43:76	Gut microbiota-metabolic axis insight into the hyperlipidemic effect of lotus seed resistant starch in hyperlipidemic mice.					
37173019	2	40	theme	norank_f_Muribaculaceae	523:545	arg1	abundance					510:518	the abundance	506:518	the abundance of norank_f_Muribaculaceae and norank_f_Erysipelotrichaceae	506:578	Allobaculum was significantly decreased in LRS groups compared to MC group, while MLRS promoted the abundance of norank_f_Muribaculaceae and norank_f_Erysipelotrichaceae.					
37173019	5	41	theme	gut	1005:1007	arg1	axis					1030:1033	gut microbiota-metabolic axis	1005:1033	gut microbiota-metabolic axis	1005:1033	Finally, MLRS regulate microbiota composition, and this promoted cholesterol catabolism to form CA, which inhibited serum lipid index by gut microbiota-metabolic axis.					
37173019	5	42	theme	microbiota-metabolic	1009:1028	arg1	axis					1030:1033	gut microbiota-metabolic axis	1005:1033	gut microbiota-metabolic axis	1005:1033	Finally, MLRS regulate microbiota composition, and this promoted cholesterol catabolism to form CA, which inhibited serum lipid index by gut microbiota-metabolic axis.					
37173019	3	43	theme	cholic	623:628	arg1	CA					636:637	CA	636:637	CA	636:637	Moreover, supplementation of LRS promoted cholic acid (CA) production and inhibited deoxycholic acid compared to MC group.					
37173019	3	43	theme	cholic	623:628	arg1	acid					630:633	cholic acid	623:633	cholic acid (CA) production	623:649	Moreover, supplementation of LRS promoted cholic acid (CA) production and inhibited deoxycholic acid compared to MC group.					
37173019	1	44	theme	model	384:388	arg1	mice					378:381	high-fat diet mice	364:381	high-fat diet mice (model control group, MC)	364:407	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	1	44	theme	model	384:388	arg1	group					398:402	model control group	384:402	model control group	384:402	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	3	45	theme	LRS	610:612	arg1	supplementation					591:605	supplementation	591:605	supplementation of LRS	591:612	Moreover, supplementation of LRS promoted cholic acid (CA) production and inhibited deoxycholic acid compared to MC group.					
37173019	6	46	theme	hyperlipidemia	1165:1178	arg1	mice					1180:1183	hyperlipidemia mice	1165:1183	hyperlipidemia mice	1165:1183	In conclusion, MLRS can promote CA and inhibit medium chain fatty acids, so as to play the best role in lowering blood lipids in hyperlipidemia mice.					
37173019	5	47	theme	microbiota	891:900	arg1	composition					902:912	microbiota composition	891:912	microbiota composition	891:912	Finally, MLRS regulate microbiota composition, and this promoted cholesterol catabolism to form CA, which inhibited serum lipid index by gut microbiota-metabolic axis.					
37173019	6	48	from	lipids	1155:1160	arg1	mice					1180:1183	hyperlipidemia mice	1165:1183	hyperlipidemia mice	1165:1183	In conclusion, MLRS can promote CA and inhibit medium chain fatty acids, so as to play the best role in lowering blood lipids in hyperlipidemia mice.					
37173019	1	49	theme	control	390:396	arg1	mice					378:381	high-fat diet mice	364:381	high-fat diet mice (model control group, MC)	364:407	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	1	49	theme	control	390:396	arg1	group					398:402	model control group	384:402	model control group	384:402	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	4	50	theme	20-Carboxy-leukotriene	753:774	arg1	B4					776:777	20-Carboxy-leukotriene B4	753:777	20-Carboxy-leukotriene B4	753:777	Among, LLRS promoted formic acid, MLRS inhibited 20-Carboxy-leukotriene B4, while HLRS promoted 3, 4-Methyleneazelaic acid and inhibited Oleic acid and Malic acid.					
37173019	1	51	theme	medium	223:228	arg1	MLRS					264:267	low-, medium and high-dose LRS, named as LLRS, MLRS	217:267	low-, medium and high-dose LRS, named as LLRS, MLRS	217:267	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	0	52	theme	lotus	72:76	arg1	effect					62:67	the hyperlipidemic effect	43:67	the hyperlipidemic effect of lotus	43:76	Gut microbiota-metabolic axis insight into the hyperlipidemic effect of lotus seed resistant starch in hyperlipidemic mice.					
37173019	1	53	theme	hyperlipidemic	144:157	arg1	effect					159:164	the hyperlipidemic effect	140:164	the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice	140:314	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
37173019	6	54	theme	blood	1149:1153	arg1	lipids					1155:1160	blood lipids	1149:1160	blood lipids in hyperlipidemia mice	1149:1183	In conclusion, MLRS can promote CA and inhibit medium chain fatty acids, so as to play the best role in lowering blood lipids in hyperlipidemia mice.					
37173019	1	55	theme	high-dose	234:242	arg1	LRS					244:246	high-dose LRS	234:246	high-dose LRS	234:246	We investigated the hyperlipidemic effect of different doses of lotus seed resistant starch (low-, medium and high-dose LRS, named as LLRS, MLRS and HLRS, respectively) in hyperlipidemic mice using gut microbiota-metabolic axis compared to high-fat diet mice (model control group, MC).					
36202660	6	0	theme	conversion	1573:1582	arg1	sorption					1550:1557	their water sorption	1538:1557	their water sorption	1538:1557	Adhesives filled with ChNC-C10 enhanced their flexural strength, microhardness, and thermal stability and decreased their water sorption and degree of conversion.					
36202660	6	0	theme	conversion	1573:1582	arg1	degree					1563:1568	degree	1563:1568	degree of conversion	1563:1582	Adhesives filled with ChNC-C10 enhanced their flexural strength, microhardness, and thermal stability and decreased their water sorption and degree of conversion.					
36202660	1	1	theme	photopolymerizable	258:275	arg1	adhesives					291:299	photopolymerizable dental filled adhesives	258:299	photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties	258:400	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	4	2	theme	shrimp	1142:1147	arg1	shells					1149:1154	shrimp shells	1142:1154	shrimp shells	1142:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	3	3	theme	NMR	812:814	arg1	spectroscopy					816:827	solid-state NMR spectroscopy	800:827	solid-state NMR spectroscopy	800:827	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	8	4	theme	physical	1833:1840	arg1	properties					1857:1866	the improved physical and mechanical properties	1820:1866	the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals	1820:1958	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	1	5	theme	filled	284:289	arg1	adhesives					291:299	photopolymerizable dental filled adhesives	258:299	photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties	258:400	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	8	6	theme	mechanical	1846:1855	arg1	properties					1857:1866	the improved physical and mechanical properties	1820:1866	the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals	1820:1958	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	8	7	theme	low/moderate	2042:2053	arg1	ChNC-C10					2066:2073	ChNC-C10	2066:2073	ChNC-C10	2066:2073	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	8	7	theme	low/moderate	2042:2053	arg1	amounts					2055:2061	low/moderate amounts	2042:2061	low/moderate amounts of ChNC-C10	2042:2073	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	1	8	theme	chitin	205:210	arg1	nanocrystals					212:223	pristine and functionalized chitin nanocrystals	177:223	pristine and functionalized chitin nanocrystals	177:223	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	7	9	theme	flexural	1754:1761	arg1	strength					1763:1770	their flexural strength	1748:1770	their flexural strength	1748:1770	Adhesives filled with ChNC-MA resulted in improvements in microhardness, in water sorption and degree of conversion, although they did not exhibit augmentation of their flexural strength and thermal stability.					
36202660	4	10	theme	surface	1200:1206	arg1	preparation					1064:1074	the successful preparation	1049:1074	the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells	1049:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	4	10	theme	surface	1200:1206	arg1	hydrophobization					1174:1189	the efficient hydrophobization	1160:1189	the efficient hydrophobization of their surface	1160:1206	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	3	11	theme	%	682:682	arg1	concentrations					654:667	concentrations	654:667	concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests	654:1002	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	8	12	theme	control	1888:1894	arg1	adhesives					1903:1911	the control, resin adhesives	1884:1911	the control, resin adhesives filled with anti-bacterial chitin nanocrystals	1884:1958	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	3	13	dep	materials	698:706	arg1	properties					733:742	physical and mechanical properties	709:742	the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests	694:1002	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	3	14	theme	scanning	875:882	arg1	SEM					905:907	SEM	905:907	SEM	905:907	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	3	14	theme	scanning	875:882	arg1	microscopy					893:902	scanning electron microscopy	875:902	scanning electron microscopy (SEM)	875:908	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	1	15	dep	OBJECTIVES	106:115	arg1	propose					134:140	propose	134:140	propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties	134:400	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	5	16	theme	layered	1318:1324	arg1	structures					1326:1335	layered structures	1318:1335	layered structures	1318:1335	Electron microscope images indicated that the increased hydrophobicity of ChNC-C10 promotes the formation of layered structures throughout the resin adhesive, while ChNC-MA tends to form aggregates in the matrix.					
36202660	5	17	theme	Electron	1209:1216	arg1	images					1229:1234	Electron microscope images	1209:1234	Electron microscope images	1209:1234	Electron microscope images indicated that the increased hydrophobicity of ChNC-C10 promotes the formation of layered structures throughout the resin adhesive, while ChNC-MA tends to form aggregates in the matrix.					
36202660	0	18	theme	mechanical	13:22	arg1	properties					24:33	Physical and mechanical properties	0:33	Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals	0:103	Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals.					
36202660	8	19	theme	anti-bacterial	1925:1938	arg1	nanocrystals					1947:1958	anti-bacterial chitin nanocrystals	1925:1958	anti-bacterial chitin nanocrystals	1925:1958	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	1	20	theme	natural	152:158	arg1	nanocrystals					212:223	pristine and functionalized chitin nanocrystals	177:223	pristine and functionalized chitin nanocrystals	177:223	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	1	20	theme	natural	152:158	arg1	fillers					160:166	embedding natural fillers	142:166	embedding natural fillers	142:166	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	4	21	theme	successful	1053:1062	arg1	preparation					1064:1074	the successful preparation	1049:1074	the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells	1049:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	0	22	theme	hydrophobic	73:83	arg1	nanocrystals					92:103	hydrophobic chitin nanocrystals	73:103	hydrophobic chitin nanocrystals	73:103	Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals.					
36202660	7	23	theme	conversion	1690:1699	arg1	degree					1680:1685	degree	1680:1685	degree of conversion	1680:1699	Adhesives filled with ChNC-MA resulted in improvements in microhardness, in water sorption and degree of conversion, although they did not exhibit augmentation of their flexural strength and thermal stability.					
36202660	7	23	theme	conversion	1690:1699	arg1	sorption					1667:1674	water sorption	1661:1674	water sorption	1661:1674	Adhesives filled with ChNC-MA resulted in improvements in microhardness, in water sorption and degree of conversion, although they did not exhibit augmentation of their flexural strength and thermal stability.					
36202660	8	24	from	SIGNIFICANCE	1795:1806	arg1	light					1811:1815	light	1811:1815	light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals	1811:1958	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	8	25	theme	promising	1964:1972	arg1	materials					1978:1986	promising new materials	1964:1986	promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10	1964:2073	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	8	25	theme	promising	1964:1972	arg1	SIGNIFICANCE					1795:1806	SIGNIFICANCE	1795:1806	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals	1795:1958	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	4	26	theme	analytical	1017:1026	arg1	techniques					1028:1037	The analytical techniques	1013:1037	The analytical techniques	1013:1037	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	5	27	theme	structures	1326:1335	arg1	formation					1305:1313	the formation	1301:1313	the formation of layered structures	1301:1335	Electron microscope images indicated that the increased hydrophobicity of ChNC-C10 promotes the formation of layered structures throughout the resin adhesive, while ChNC-MA tends to form aggregates in the matrix.					
36202660	4	28	theme	commercial	1104:1113	arg1	powder					1122:1127	commercial chitin powder	1104:1127	commercial chitin powder derived from shrimp shells	1104:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	0	29	theme	Physical	0:7	arg1	properties					24:33	Physical and mechanical properties	0:33	Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals	0:103	Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals.					
36202660	4	30	from	powder	1122:1127	arg1	preparation					1064:1074	the successful preparation	1049:1074	the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells	1049:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	4	30	from	powder	1122:1127	arg1	nanocrystals					1086:1097	chitin nanocrystals	1079:1097	chitin nanocrystals from commercial chitin powder derived from shrimp shells	1079:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	4	30	from	powder	1122:1127	arg1	hydrophobization					1174:1189	the efficient hydrophobization	1160:1189	the efficient hydrophobization of their surface	1160:1206	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	8	31	theme	dental	1992:1997	arg1	applications					1999:2010	dental applications	1992:2010	dental applications	1992:2010	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	8	31	theme	dental	1992:1997	arg1	those					2024:2028	those	2024:2028	those	2024:2028	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	3	32	theme	sorption	989:996	arg1	tests					998:1002	water sorption tests	983:1002	water sorption tests	983:1002	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	2	33	theme	decanoyl	463:470	arg1	chloride					472:479	decanoyl chloride	463:479	decanoyl chloride	463:479	METHODS Chitin nanocrystals (ChNC) were functionalized with decanoyl chloride and methacrylic anhydride to produce ChNC-C10 and ChNC-MA, respectively.					
36202660	6	34	theme	water	1544:1548	arg1	sorption					1550:1557	their water sorption	1538:1557	their water sorption	1538:1557	Adhesives filled with ChNC-C10 enhanced their flexural strength, microhardness, and thermal stability and decreased their water sorption and degree of conversion.					
36202660	1	35	theme	enhanced	306:313	arg1	biocompatibility					315:330	biocompatibility	315:330	biocompatibility	315:330	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	7	36	theme	water	1661:1665	arg1	sorption					1667:1674	water sorption	1661:1674	water sorption	1661:1674	Adhesives filled with ChNC-MA resulted in improvements in microhardness, in water sorption and degree of conversion, although they did not exhibit augmentation of their flexural strength and thermal stability.					
36202660	7	37	theme	stability	1784:1792	arg1	augmentation					1732:1743	augmentation	1732:1743	augmentation of their flexural strength and thermal stability	1732:1792	Adhesives filled with ChNC-MA resulted in improvements in microhardness, in water sorption and degree of conversion, although they did not exhibit augmentation of their flexural strength and thermal stability.					
36202660	7	38	theme	thermal	1776:1782	arg1	stability					1784:1792	thermal stability	1776:1792	thermal stability	1776:1792	Adhesives filled with ChNC-MA resulted in improvements in microhardness, in water sorption and degree of conversion, although they did not exhibit augmentation of their flexural strength and thermal stability.					
36202660	0	39	theme	adhesive	53:60	arg1	resin					47:51	a dental resin adhesive	38:60	a dental resin adhesive containing hydrophobic chitin nanocrystals	38:103	Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals.					
36202660	3	40	theme	mechanical	722:731	arg1	properties					733:742	physical and mechanical properties	709:742	the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests	694:1002	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	4	41	theme	nanocrystals	1086:1097	arg1	preparation					1064:1074	the successful preparation	1049:1074	the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells	1049:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	4	41	theme	nanocrystals	1086:1097	arg1	hydrophobization					1174:1189	the efficient hydrophobization	1160:1189	the efficient hydrophobization of their surface	1160:1206	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	3	42	theme	physical	709:716	arg1	properties					733:742	physical and mechanical properties	709:742	the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests	694:1002	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	0	43	theme	dental	40:45	arg1	resin					47:51	a dental resin adhesive	38:60	a dental resin adhesive containing hydrophobic chitin nanocrystals	38:103	Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals.					
36202660	5	44	theme	increased	1255:1263	arg1	hydrophobicity					1265:1278	the increased hydrophobicity	1251:1278	the increased hydrophobicity of ChNC-C10	1251:1290	Electron microscope images indicated that the increased hydrophobicity of ChNC-C10 promotes the formation of layered structures throughout the resin adhesive, while ChNC-MA tends to form aggregates in the matrix.					
36202660	3	45	theme	infrared	770:777	arg1	spectroscopy					786:797	Fourier-transform infrared (FTIR) spectroscopy	752:797	Fourier-transform infrared (FTIR) spectroscopy	752:797	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	3	45	theme	infrared	770:777	arg1	FTIR					780:783	FTIR	780:783	FTIR	780:783	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	3	46	theme	functionalized	576:589	arg1	nanocrystals					598:609	These hydrophobically functionalized chitin nanocrystals	554:609	These hydrophobically functionalized chitin nanocrystals	554:609	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	7	47	from	improvements	1627:1638	arg1	microhardness					1643:1655	microhardness	1643:1655	microhardness	1643:1655	Adhesives filled with ChNC-MA resulted in improvements in microhardness, in water sorption and degree of conversion, although they did not exhibit augmentation of their flexural strength and thermal stability.					
36202660	0	48	contain	containing	62:71	arg2	nanocrystals					92:103	hydrophobic chitin nanocrystals	73:103	hydrophobic chitin nanocrystals	73:103	Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals.					
36202660	0	48	contain	containing	62:71	arg1	resin					47:51	a dental resin adhesive	38:60	a dental resin adhesive containing hydrophobic chitin nanocrystals	38:103	Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals.					
36202660	8	49	dep	control	1888:1894	arg1	resin					1897:1901	resin	1897:1901	resin	1897:1901	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	1	50	theme	dental	277:282	arg1	adhesives					291:299	photopolymerizable dental filled adhesives	258:299	photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties	258:400	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	8	51	theme	improved	1824:1831	arg1	properties					1857:1866	the improved physical and mechanical properties	1820:1866	the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals	1820:1958	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	3	52	theme	solid-state	800:810	arg1	spectroscopy					816:827	solid-state NMR spectroscopy	800:827	solid-state NMR spectroscopy	800:827	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	3	53	theme	adhesive	642:649	arg1	resin					636:640	a resin	634:640	a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests	634:1002	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	1	54	theme	pristine	177:184	arg1	nanocrystals					212:223	pristine and functionalized chitin nanocrystals	177:223	pristine and functionalized chitin nanocrystals	177:223	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	7	55	theme	strength	1763:1770	arg1	augmentation					1732:1743	augmentation	1732:1743	augmentation of their flexural strength and thermal stability	1732:1792	Adhesives filled with ChNC-MA resulted in improvements in microhardness, in water sorption and degree of conversion, although they did not exhibit augmentation of their flexural strength and thermal stability.					
36202660	1	56	with	adhesives	291:299	arg1	hydrophobicity					333:346	hydrophobicity	333:346	hydrophobicity	333:346	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	1	56	with	adhesives	291:299	arg1	resistance					360:369	mechanical resistance	349:369	mechanical resistance	349:369	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	1	56	with	adhesives	291:299	arg1	biocompatibility					315:330	biocompatibility	315:330	biocompatibility	315:330	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	1	56	with	adhesives	291:299	arg1	properties					391:400	anti-bacterial properties	376:400	anti-bacterial properties	376:400	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	8	57	theme	ChNC-C10	2066:2073	arg1	ChNC-C10					2066:2073	ChNC-C10	2066:2073	ChNC-C10	2066:2073	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	8	57	theme	ChNC-C10	2066:2073	arg1	amounts					2055:2061	low/moderate amounts	2042:2061	low/moderate amounts of ChNC-C10	2042:2073	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	8	58	theme	properties	1857:1866	arg1	light					1811:1815	light	1811:1815	light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals	1811:1958	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	1	59	theme	functionalized	190:203	arg1	nanocrystals					212:223	pristine and functionalized chitin nanocrystals	177:223	pristine and functionalized chitin nanocrystals	177:223	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	3	60	theme	X-ray	830:834	arg1	diffraction					836:846	X-ray diffraction	830:846	X-ray diffraction (XRD)	830:852	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	3	60	theme	X-ray	830:834	arg1	XRD					849:851	XRD	849:851	XRD	849:851	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	4	61	dep	RESULTS	1005:1011	arg1	confirmed					1039:1047	confirmed	1039:1047	confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface	1039:1206	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	3	62	theme	0.5-3.0 wt	672:681	arg1	%					682:682	0.5-3.0 wt%	672:682	0.5-3.0 wt%	672:682	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	1	63	theme	mechanical	349:358	arg1	resistance					360:369	mechanical resistance	349:369	mechanical resistance	349:369	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	3	64	theme	elemental	855:863	arg1	analysis					865:872	elemental analysis	855:872	elemental analysis	855:872	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	4	65	from	hydrophobization	1174:1189	arg1	powder					1122:1127	commercial chitin powder	1104:1127	commercial chitin powder derived from shrimp shells	1104:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	3	66	theme	electron	884:891	arg1	SEM					905:907	SEM	905:907	SEM	905:907	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	3	66	theme	electron	884:891	arg1	microscopy					893:902	scanning electron microscopy	875:902	scanning electron microscopy (SEM)	875:908	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	6	67	theme	thermal	1506:1512	arg1	stability					1514:1522	thermal stability	1506:1522	thermal stability	1506:1522	Adhesives filled with ChNC-C10 enhanced their flexural strength, microhardness, and thermal stability and decreased their water sorption and degree of conversion.					
36202660	1	68	theme	embedding	142:150	arg1	nanocrystals					212:223	pristine and functionalized chitin nanocrystals	177:223	pristine and functionalized chitin nanocrystals	177:223	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	1	68	theme	embedding	142:150	arg1	fillers					160:166	embedding natural fillers	142:166	embedding natural fillers	142:166	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	3	69	theme	thermogravimetric	911:927	arg1	analysis					929:936	thermogravimetric analysis	911:936	thermogravimetric analysis (TGA)	911:942	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	3	69	theme	thermogravimetric	911:927	arg1	TGA					939:941	TGA	939:941	TGA	939:941	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	0	70	theme	chitin	85:90	arg1	nanocrystals					92:103	hydrophobic chitin nanocrystals	73:103	hydrophobic chitin nanocrystals	73:103	Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals.					
36202660	5	71	theme	microscope	1218:1227	arg1	images					1229:1234	Electron microscope images	1209:1234	Electron microscope images	1209:1234	Electron microscope images indicated that the increased hydrophobicity of ChNC-C10 promotes the formation of layered structures throughout the resin adhesive, while ChNC-MA tends to form aggregates in the matrix.					
36202660	3	72	theme	flexural	945:952	arg1	strength					954:961	flexural strength	945:961	flexural strength	945:961	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	1	73	theme	anti-bacterial	376:389	arg1	properties					391:400	anti-bacterial properties	376:400	anti-bacterial properties	376:400	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	8	74	theme	chitin	1940:1945	arg1	nanocrystals					1947:1958	anti-bacterial chitin nanocrystals	1925:1958	anti-bacterial chitin nanocrystals	1925:1958	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	6	75	theme	flexural	1468:1475	arg1	strength					1477:1484	their flexural strength	1462:1484	their flexural strength	1462:1484	Adhesives filled with ChNC-C10 enhanced their flexural strength, microhardness, and thermal stability and decreased their water sorption and degree of conversion.					
36202660	4	76	attach	derived	1129:1135	arg2	powder					1122:1127	commercial chitin powder	1104:1127	commercial chitin powder derived from shrimp shells	1104:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	4	76	attach	derived	1129:1135	arg1	shells					1149:1154	shrimp shells	1142:1154	shrimp shells	1142:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	8	77	theme	new	1974:1976	arg1	materials					1978:1986	promising new materials	1964:1986	promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10	1964:2073	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	8	77	theme	new	1974:1976	arg1	SIGNIFICANCE					1795:1806	SIGNIFICANCE	1795:1806	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals	1795:1958	SIGNIFICANCE In light of the improved physical and mechanical properties with respect to the control, resin adhesives filled with anti-bacterial chitin nanocrystals are promising new materials for dental applications, especially those filled with low/moderate amounts of ChNC-C10.					
36202660	2	78	theme	methacrylic	485:495	arg1	anhydride					497:505	methacrylic anhydride	485:505	methacrylic anhydride	485:505	METHODS Chitin nanocrystals (ChNC) were functionalized with decanoyl chloride and methacrylic anhydride to produce ChNC-C10 and ChNC-MA, respectively.					
36202660	3	79	from	concentrations	654:667	arg1	adhesive					642:649	adhesive	642:649	adhesive	642:649	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	5	80	theme	ChNC-C10	1283:1290	arg1	hydrophobicity					1265:1278	the increased hydrophobicity	1251:1278	the increased hydrophobicity of ChNC-C10	1251:1290	Electron microscope images indicated that the increased hydrophobicity of ChNC-C10 promotes the formation of layered structures throughout the resin adhesive, while ChNC-MA tends to form aggregates in the matrix.					
36202660	4	81	theme	chitin	1115:1120	arg1	powder					1122:1127	commercial chitin powder	1104:1127	commercial chitin powder derived from shrimp shells	1104:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	2	82	theme	Chitin	411:416	arg1	ChNC					432:435	ChNC	432:435	ChNC	432:435	METHODS Chitin nanocrystals (ChNC) were functionalized with decanoyl chloride and methacrylic anhydride to produce ChNC-C10 and ChNC-MA, respectively.					
36202660	2	82	theme	Chitin	411:416	arg1	nanocrystals					418:429	METHODS Chitin nanocrystals	403:429	METHODS Chitin nanocrystals (ChNC)	403:436	METHODS Chitin nanocrystals (ChNC) were functionalized with decanoyl chloride and methacrylic anhydride to produce ChNC-C10 and ChNC-MA, respectively.					
36202660	3	83	theme	water	983:987	arg1	tests					998:1002	water sorption tests	983:1002	water sorption tests	983:1002	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	0	84	theme	resin	47:51	arg1	properties					24:33	Physical and mechanical properties	0:33	Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals	0:103	Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals.					
36202660	4	85	theme	chitin	1079:1084	arg1	nanocrystals					1086:1097	chitin nanocrystals	1079:1097	chitin nanocrystals from commercial chitin powder derived from shrimp shells	1079:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	3	86	theme	Fourier-transform	752:768	arg1	spectroscopy					786:797	Fourier-transform infrared (FTIR) spectroscopy	752:797	Fourier-transform infrared (FTIR) spectroscopy	752:797	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	3	86	theme	Fourier-transform	752:768	arg1	FTIR					780:783	FTIR	780:783	FTIR	780:783	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36202660	1	87	from	OBJECTIVES	106:115	arg1	paper					125:129	this paper	120:129	this paper	120:129	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	4	88	from	preparation	1064:1074	arg1	powder					1122:1127	commercial chitin powder	1104:1127	commercial chitin powder derived from shrimp shells	1104:1154	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	2	89	theme	METHODS	403:409	arg1	ChNC					432:435	ChNC	432:435	ChNC	432:435	METHODS Chitin nanocrystals (ChNC) were functionalized with decanoyl chloride and methacrylic anhydride to produce ChNC-C10 and ChNC-MA, respectively.					
36202660	2	89	theme	METHODS	403:409	arg1	nanocrystals					418:429	METHODS Chitin nanocrystals	403:429	METHODS Chitin nanocrystals (ChNC)	403:436	METHODS Chitin nanocrystals (ChNC) were functionalized with decanoyl chloride and methacrylic anhydride to produce ChNC-C10 and ChNC-MA, respectively.					
36202660	1	90	theme	resin	231:235	arg1	adhesives					237:245	resin adhesives	231:245	resin adhesives	231:245	OBJECTIVES In this paper we propose embedding natural fillers, such as pristine and functionalized chitin nanocrystals, into resin adhesives to produce photopolymerizable dental filled adhesives with enhanced biocompatibility, hydrophobicity, mechanical resistance, and anti-bacterial properties.					
36202660	4	91	theme	efficient	1164:1172	arg1	hydrophobization					1174:1189	the efficient hydrophobization	1160:1189	the efficient hydrophobization of their surface	1160:1206	RESULTS The analytical techniques confirmed the successful preparation of chitin nanocrystals from commercial chitin powder derived from shrimp shells and the efficient hydrophobization of their surface.					
36202660	3	92	theme	chitin	591:596	arg1	nanocrystals					598:609	These hydrophobically functionalized chitin nanocrystals	554:609	These hydrophobically functionalized chitin nanocrystals	554:609	These hydrophobically functionalized chitin nanocrystals were incorporated into a resin adhesive at concentrations of 0.5-3.0 wt% to assess the materials' physical and mechanical properties through Fourier-transform infrared (FTIR) spectroscopy, solid-state NMR spectroscopy, X-ray diffraction (XRD), elemental analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexural strength, microhardness, and water sorption tests.					
36493486	0	0	theme	carboxylated	82:93	arg1	carbon					95:100	carboxylated carbon	82:100	carboxylated carbon	82:100	Preparation of an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon nano-tubes for extraction of pesticides from food and agricultural water samples before GC-FID analysis.					
36493486	4	1	theme	food	1283:1286	arg1	samples					1288:1294	various agricultural water and food samples	1252:1294	samples	1288:1294	Ultimately, the obtained results showed that the developed method could be applied to determine trace amounts of desired analytes in various agricultural water and food samples.					
36493486	0	2	theme	GC-FID	190:195	arg1	analysis					197:204	GC-FID analysis	190:204	GC-FID analysis	190:204	Preparation of an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon nano-tubes for extraction of pesticides from food and agricultural water samples before GC-FID analysis.					
36493486	2	3	theme	extraction	594:603	arg1	conditions					605:614	the optimized extraction conditions	580:614	the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride)	580:834	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	4	theme	w/v	815:817	arg1	chloride					826:833	5.0 % w/v sodium chloride	809:833	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	0	5	from	samples	175:181	arg1	extraction					117:126	extraction	117:126	extraction of pesticides from food and agricultural water samples before GC-FID analysis	117:204	Preparation of an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon nano-tubes for extraction of pesticides from food and agricultural water samples before GC-FID analysis.					
36493486	1	6	theme	@	357:357	arg1	CNT-COOH					358:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	6	theme	@	357:357	arg1	sorbent					383:389	an effectual sorbent	370:389	an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	370:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	4	7	theme	trace	1215:1219	arg1	amounts					1221:1227	trace amounts	1215:1227	trace amounts of desired analytes in various agricultural water and food samples	1215:1294	Ultimately, the obtained results showed that the developed method could be applied to determine trace amounts of desired analytes in various agricultural water and food samples.					
36493486	4	7	theme	trace	1215:1219	arg1	analytes					1240:1247	desired analytes	1232:1247	desired analytes	1232:1247	Ultimately, the obtained results showed that the developed method could be applied to determine trace amounts of desired analytes in various agricultural water and food samples.					
36493486	1	8	theme	herbicides	452:461	arg1	co-extraction					399:411	the co-extraction	395:411	the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	395:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	2	9	theme	%	813:813	arg1	chloride					826:833	5.0 % w/v sodium chloride	809:833	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	1	10	theme	functionalized	271:284	arg1	nano-composite					295:308	carboxamide functionalized magnetic nano-composite	259:308	carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	259:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	11	from	co-extraction	399:411	arg1	samples					496:502	vegetable, fruit, and water samples	468:502	samples	496:502	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	11	from	co-extraction	399:411	arg1	fruit					479:483	vegetable, fruit, and water samples	468:502	fruit	479:483	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	3	12	theme	acceptable	1015:1024	arg1	recoveries					1035:1044	acceptable relative recoveries	1015:1044	acceptable relative recoveries (87.6-103.8 %)	1015:1059	Extraction efficiencies were in the range 63.4-84.1 %, the limits of detection were 0.08-1.0 ng/mL, and acceptable relative recoveries (87.6-103.8 %), and precisions were also achieved (RSDs < 6.8 %, n = 3).					
36493486	3	12	theme	acceptable	1015:1024	arg1	%					1058:1058	87.6-103.8 %	1047:1058	87.6-103.8 %	1047:1058	Extraction efficiencies were in the range 63.4-84.1 %, the limits of detection were 0.08-1.0 ng/mL, and acceptable relative recoveries (87.6-103.8 %), and precisions were also achieved (RSDs < 6.8 %, n = 3).					
36493486	2	13	theme	desorption	668:677	arg1	volume					687:692	desorption solvent volume	668:692	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	14	theme	extraction	737:746	arg1	time					748:751	extraction time	737:751	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	1	15	theme	magnetic	286:293	arg1	nano-composite					295:308	carboxamide functionalized magnetic nano-composite	259:308	carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	259:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	4	16	theme	various	1252:1258	arg1	water					1273:1277	various agricultural water and food samples	1252:1294	water	1273:1277	Ultimately, the obtained results showed that the developed method could be applied to determine trace amounts of desired analytes in various agricultural water and food samples.					
36493486	3	17	theme	relative	1026:1033	arg1	recoveries					1035:1044	acceptable relative recoveries	1015:1044	acceptable relative recoveries (87.6-103.8 %)	1015:1059	Extraction efficiencies were in the range 63.4-84.1 %, the limits of detection were 0.08-1.0 ng/mL, and acceptable relative recoveries (87.6-103.8 %), and precisions were also achieved (RSDs < 6.8 %, n = 3).					
36493486	3	17	theme	relative	1026:1033	arg1	%					1058:1058	87.6-103.8 %	1047:1058	87.6-103.8 %	1047:1058	Extraction efficiencies were in the range 63.4-84.1 %, the limits of detection were 0.08-1.0 ng/mL, and acceptable relative recoveries (87.6-103.8 %), and precisions were also achieved (RSDs < 6.8 %, n = 3).					
36493486	1	18	theme	vegetable	468:476	arg1	fruit					479:483	vegetable, fruit, and water samples	468:502	fruit	479:483	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	2	19	theme	sample	769:774	arg1	solution					776:783	sample solution	769:783	sample solution	769:783	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	1	20	theme	nano-composite	295:308	arg1	approach					247:254	the direct synthesis approach	226:254	the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	226:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	2	21	theme	6.5 min	659:665	arg1	18.1 mg					633:639	18.1 mg	633:639	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	3	22	dep	achieved	1087:1094	arg1	n = 3					1111:1115	n = 3	1111:1115	n = 3	1111:1115	Extraction efficiencies were in the range 63.4-84.1 %, the limits of detection were 0.08-1.0 ng/mL, and acceptable relative recoveries (87.6-103.8 %), and precisions were also achieved (RSDs < 6.8 %, n = 3).					
36493486	3	22	dep	achieved	1087:1094	arg1	%					1108:1108	RSDs < 6.8 %	1097:1108	RSDs < 6.8 %	1097:1108	Extraction efficiencies were in the range 63.4-84.1 %, the limits of detection were 0.08-1.0 ng/mL, and acceptable relative recoveries (87.6-103.8 %), and precisions were also achieved (RSDs < 6.8 %, n = 3).					
36493486	1	23	theme	effectual	373:381	arg1	CNT-COOH					358:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	23	theme	effectual	373:381	arg1	sorbent					383:389	an effectual sorbent	370:389	an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	370:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	24	theme	carboxamide	259:269	arg1	nano-composite					295:308	carboxamide functionalized magnetic nano-composite	259:308	carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	259:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	2	25	theme	desorption	703:712	arg1	solvent					714:720	desorption solvent	703:720	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	26	theme	sorbent	617:623	arg1	amount					625:630	sorbent amount	617:630	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	4	27	theme	agricultural	1260:1271	arg1	water					1273:1277	various agricultural water and food samples	1252:1294	water	1273:1277	Ultimately, the obtained results showed that the developed method could be applied to determine trace amounts of desired analytes in various agricultural water and food samples.					
36493486	2	28	theme	time	653:656	arg1	18.1 mg					633:639	18.1 mg	633:639	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	29	theme	desorption	642:651	arg1	time					653:656	desorption time	642:656	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	30	theme	solvent	679:685	arg1	volume					687:692	desorption solvent volume	668:692	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	0	31	theme	efficient	18:26	arg1	nano-sorbent					37:48	an efficient magnetic nano-sorbent	15:48	an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon	15:100	Preparation of an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon nano-tubes for extraction of pesticides from food and agricultural water samples before GC-FID analysis.					
36493486	1	32	theme	Fe3O4	316:320	arg1	CNT-COOH					358:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	32	theme	Fe3O4	316:320	arg1	sorbent					383:389	an effectual sorbent	370:389	an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	370:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	0	33	theme	pesticides	131:140	arg1	extraction					117:126	extraction	117:126	extraction of pesticides from food and agricultural water samples before GC-FID analysis	117:204	Preparation of an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon nano-tubes for extraction of pesticides from food and agricultural water samples before GC-FID analysis.					
36493486	1	34	theme	water	490:494	arg1	samples					496:502	vegetable, fruit, and water samples	468:502	samples	496:502	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	2	35	theme	volume	687:692	arg1	18.1 mg					633:639	18.1 mg	633:639	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	1	36	theme	@	321:321	arg1	CNT-COOH					358:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	36	theme	@	321:321	arg1	sorbent					383:389	an effectual sorbent	370:389	an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	370:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	2	37	theme	range	863:867	arg1	R2 ≥ 0.998					885:894	R2 ≥ 0.998	885:894	R2 ≥ 0.998	885:894	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	37	theme	range	863:867	arg1	0.5-1200 ng/mL					869:882	the range 0.5-1200 ng/mL	859:882	the range 0.5-1200 ng/mL (R2 ≥ 0.998)	859:895	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	0	38	theme	nano-sorbent	37:48	arg1	Preparation					0:10	Preparation	0:10	Preparation of an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon	0:100	Preparation of an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon nano-tubes for extraction of pesticides from food and agricultural water samples before GC-FID analysis.					
36493486	1	39	theme	SiO2-NH2	322:329	arg1	CNT-COOH					358:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	39	theme	SiO2-NH2	322:329	arg1	sorbent					383:389	an effectual sorbent	370:389	an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	370:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	0	40	theme	food	147:150	arg1	samples					175:181	food and agricultural water samples	147:181	food and agricultural water samples before GC-FID analysis	147:204	Preparation of an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon nano-tubes for extraction of pesticides from food and agricultural water samples before GC-FID analysis.					
36493486	2	41	theme	solution	776:783	arg1	content					800:806	salt content	795:806	salt content	795:806	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	41	theme	solution	776:783	arg1	pH					763:764	pH	763:764	pH of sample solution	763:783	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	41	theme	solution	776:783	arg1	9.5 min					754:760	9.5 min	754:760	9.5 min	754:760	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	1	42	from	samples	496:502	arg1	co-extraction					399:411	the co-extraction	395:411	the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	395:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	42	from	samples	496:502	arg1	herbicides					452:461	herbicides	452:461	herbicides	452:461	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	42	from	samples	496:502	arg1	insecticides					435:446	seven agricultural insecticides	416:446	seven agricultural insecticides	416:446	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	0	43	theme	magnetic	28:35	arg1	nano-sorbent					37:48	an efficient magnetic nano-sorbent	15:48	an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon	15:100	Preparation of an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon nano-tubes for extraction of pesticides from food and agricultural water samples before GC-FID analysis.					
36493486	1	44	theme	@	330:330	arg1	CNT-COOH					358:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	44	theme	@	330:330	arg1	sorbent					383:389	an effectual sorbent	370:389	an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	370:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	2	45	dep	amount	625:630	arg1	18.1 mg					633:639	18.1 mg	633:639	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	45	dep	amount	625:630	arg1	content					800:806	salt content	795:806	salt content	795:806	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	45	dep	amount	625:630	arg1	chloride					826:833	5.0 % w/v sodium chloride	809:833	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	45	dep	amount	625:630	arg1	time					748:751	extraction time	737:751	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	45	dep	amount	625:630	arg1	solvent					714:720	desorption solvent	703:720	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	45	dep	amount	625:630	arg1	9.5 min					754:760	9.5 min	754:760	9.5 min	754:760	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	45	dep	amount	625:630	arg1	7.0					786:788	7.0	786:788	7.0	786:788	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	45	dep	amount	625:630	arg1	acetonitrile					723:734	acetonitrile	723:734	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	45	dep	amount	625:630	arg1	pH					763:764	pH	763:764	pH of sample solution	763:783	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	4	46	theme	analytes	1240:1247	arg1	amounts					1221:1227	trace amounts	1215:1227	trace amounts of desired analytes in various agricultural water and food samples	1215:1294	Ultimately, the obtained results showed that the developed method could be applied to determine trace amounts of desired analytes in various agricultural water and food samples.					
36493486	4	46	theme	analytes	1240:1247	arg1	analytes					1240:1247	desired analytes	1232:1247	desired analytes	1232:1247	Ultimately, the obtained results showed that the developed method could be applied to determine trace amounts of desired analytes in various agricultural water and food samples.					
36493486	1	47	theme	dialdehyde	331:340	arg1	CNT-COOH					358:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	47	theme	dialdehyde	331:340	arg1	sorbent					383:389	an effectual sorbent	370:389	an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	370:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	0	48	theme	agricultural	156:167	arg1	water					169:173	agricultural water	156:173	agricultural water	156:173	Preparation of an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon nano-tubes for extraction of pesticides from food and agricultural water samples before GC-FID analysis.					
36493486	4	49	theme	desired	1232:1238	arg1	analytes					1240:1247	desired analytes	1232:1247	desired analytes	1232:1247	Ultimately, the obtained results showed that the developed method could be applied to determine trace amounts of desired analytes in various agricultural water and food samples.					
36493486	4	50	from	amounts	1221:1227	arg1	water					1273:1277	various agricultural water and food samples	1252:1294	water	1273:1277	Ultimately, the obtained results showed that the developed method could be applied to determine trace amounts of desired analytes in various agricultural water and food samples.					
36493486	4	50	from	amounts	1221:1227	arg1	samples					1288:1294	various agricultural water and food samples	1252:1294	samples	1288:1294	Ultimately, the obtained results showed that the developed method could be applied to determine trace amounts of desired analytes in various agricultural water and food samples.					
36493486	1	51	theme	cellulose	342:350	arg1	CNT-COOH					358:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	51	theme	cellulose	342:350	arg1	sorbent					383:389	an effectual sorbent	370:389	an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	370:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	2	52	theme	optimized	584:592	arg1	conditions					605:614	the optimized extraction conditions	580:614	the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride)	580:834	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	4	53	theme	obtained	1135:1142	arg1	results					1144:1150	the obtained results	1131:1150	the obtained results	1131:1150	Ultimately, the obtained results showed that the developed method could be applied to determine trace amounts of desired analytes in various agricultural water and food samples.					
36493486	1	54	theme	agricultural	422:433	arg1	insecticides					435:446	seven agricultural insecticides	416:446	seven agricultural insecticides	416:446	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	55	theme	magnetic	514:521	arg1	procedure					563:571	the magnetic dispersive micro-solid-phase extraction procedure	510:571	the magnetic dispersive micro-solid-phase extraction procedure	510:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	56	from	fruit	479:483	arg1	co-extraction					399:411	the co-extraction	395:411	the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	395:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	56	from	fruit	479:483	arg1	herbicides					452:461	herbicides	452:461	herbicides	452:461	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	56	from	fruit	479:483	arg1	insecticides					435:446	seven agricultural insecticides	416:446	seven agricultural insecticides	416:446	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	3	57	theme	range	947:951	arg1	%					963:963	the range 63.4-84.1 %	943:963	the range 63.4-84.1 %	943:963	Extraction efficiencies were in the range 63.4-84.1 %, the limits of detection were 0.08-1.0 ng/mL, and acceptable relative recoveries (87.6-103.8 %), and precisions were also achieved (RSDs < 6.8 %, n = 3).					
36493486	1	58	theme	direct	230:235	arg1	approach					247:254	the direct synthesis approach	226:254	the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	226:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	0	59	theme	water	169:173	arg1	samples					175:181	food and agricultural water samples	147:181	food and agricultural water samples before GC-FID analysis	147:204	Preparation of an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon nano-tubes for extraction of pesticides from food and agricultural water samples before GC-FID analysis.					
36493486	1	60	theme	insecticides	435:446	arg1	co-extraction					399:411	the co-extraction	395:411	the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	395:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	61	theme	dispersive	523:532	arg1	procedure					563:571	the magnetic dispersive micro-solid-phase extraction procedure	510:571	the magnetic dispersive micro-solid-phase extraction procedure	510:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	3	62	theme	Extraction	911:920	arg1	efficiencies					922:933	Extraction efficiencies	911:933	Extraction efficiencies	911:933	Extraction efficiencies were in the range 63.4-84.1 %, the limits of detection were 0.08-1.0 ng/mL, and acceptable relative recoveries (87.6-103.8 %), and precisions were also achieved (RSDs < 6.8 %, n = 3).					
36493486	2	63	theme	salt	795:798	arg1	content					800:806	salt content	795:806	salt content	795:806	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	1	64	theme	synthesis	237:245	arg1	approach					247:254	the direct synthesis approach	226:254	the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	226:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	2	65	dep	conditions	605:614	arg1	amount					625:630	sorbent amount	617:630	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	3	66	theme	detection	980:988	arg1	0.08-1.0 ng/mL					995:1008	0.08-1.0 ng/mL	995:1008	0.08-1.0 ng/mL	995:1008	Extraction efficiencies were in the range 63.4-84.1 %, the limits of detection were 0.08-1.0 ng/mL, and acceptable relative recoveries (87.6-103.8 %), and precisions were also achieved (RSDs < 6.8 %, n = 3).					
36493486	3	66	theme	detection	980:988	arg1	limits					970:975	the limits	966:975	the limits of detection	966:988	Extraction efficiencies were in the range 63.4-84.1 %, the limits of detection were 0.08-1.0 ng/mL, and acceptable relative recoveries (87.6-103.8 %), and precisions were also achieved (RSDs < 6.8 %, n = 3).					
36493486	1	67	theme	micro-solid-phase	534:550	arg1	procedure					563:571	the magnetic dispersive micro-solid-phase extraction procedure	510:571	the magnetic dispersive micro-solid-phase extraction procedure	510:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	4	68	theme	developed	1168:1176	arg1	method					1178:1183	the developed method	1164:1183	the developed method	1164:1183	Ultimately, the obtained results showed that the developed method could be applied to determine trace amounts of desired analytes in various agricultural water and food samples.					
36493486	0	69	theme	modified	59:66	arg1	cellulose					68:76	modified cellulose	59:76	modified cellulose	59:76	Preparation of an efficient magnetic nano-sorbent based on modified cellulose and carboxylated carbon nano-tubes for extraction of pesticides from food and agricultural water samples before GC-FID analysis.					
36493486	1	70	theme	DAC	353:355	arg1	CNT-COOH					358:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH	316:365	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	70	theme	DAC	353:355	arg1	sorbent					383:389	an effectual sorbent	370:389	an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure	370:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	1	71	theme	extraction	552:561	arg1	procedure					563:571	the magnetic dispersive micro-solid-phase extraction procedure	510:571	the magnetic dispersive micro-solid-phase extraction procedure	510:571	This paper reports the direct synthesis approach of carboxamide functionalized magnetic nano-composite named Fe3O4@SiO2-NH2@dialdehyde cellulose (DAC)@CNT-COOH as an effectual sorbent for the co-extraction of seven agricultural insecticides and herbicides from vegetable, fruit, and water samples using the magnetic dispersive micro-solid-phase extraction procedure.					
36493486	2	72	theme	good	837:840	arg1	linearity					842:850	good linearity	837:850	good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998)	837:895	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36493486	2	73	theme	sodium	819:824	arg1	chloride					826:833	5.0 % w/v sodium chloride	809:833	sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride	617:833	Under the optimized extraction conditions (sorbent amount: 18.1 mg; desorption time: 6.5 min; desorption solvent volume: 185 μL; desorption solvent: acetonitrile; extraction time: 9.5 min; pH of sample solution: 7.0, and salt content: 5.0 % w/v sodium chloride), good linearity within the range 0.5-1200 ng/mL (R2 ≥ 0.998) was achieved.					
36181897	0	0	theme	alginate	81:88	arg1	microbeads					90:99	caged alginate microbeads	75:99	caged alginate microbeads	75:99	Removal of lead ions from wastewater using magnesium sulfide nanoparticles caged alginate microbeads.					
36181897	7	1	theme	adsorption	1060:1069	arg1	equilibrium					1071:1081	the adsorption equilibrium	1056:1081	the adsorption equilibrium of Alg@MgS	1056:1092	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	1	2	theme	alginate	138:145	arg1	nanoparticles					177:189	alginate (Alg) caged magnesium sulfide nanoparticles	138:189	alginate (Alg) caged magnesium sulfide nanoparticles (MgS)	138:195	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	1	2	theme	alginate	138:145	arg1	MgS					192:194	MgS	192:194	MgS	192:194	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	4	3	theme	adsorption	483:492	arg1	conditions					494:503	The adsorption conditions	479:503	The adsorption conditions	479:503	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	0	4	theme	caged	75:79	arg1	microbeads					90:99	caged alginate microbeads	75:99	caged alginate microbeads	75:99	Removal of lead ions from wastewater using magnesium sulfide nanoparticles caged alginate microbeads.					
36181897	5	5	theme	Pb2+	798:801	arg1	ions					803:806	Pb2+ ions	798:806	Pb2+ ions	798:806	This is accomplished by removing the maximum amount of Pb2+ ions.					
36181897	7	6	theme	sodium	964:969	arg1	alginate					971:978	sodium alginate	964:978	sodium alginate (SA) for excellent metal chelation	964:1013	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	7	6	theme	sodium	964:969	arg1	SA					981:982	SA	981:982	SA	981:982	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	4	7	theme	lead	645:648	arg1	ions					650:653	lead ions	645:653	lead ions	645:653	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	1	8	theme	lead	227:230	arg1	ions					243:246	Pb2+ ions	238:246	Pb2+ ions	238:246	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	1	8	theme	lead	227:230	arg1	ions					232:235	lead ions	227:235	lead ions (Pb2+ ions)	227:247	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	7	9	theme	alginate	971:978	arg1	ability					953:959	the ability	949:959	the ability of sodium alginate (SA) for excellent metal chelation	949:1013	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	7	9	theme	alginate	971:978	arg1	structure					1045:1053	controlled nanosized pore structure	1019:1053	controlled nanosized pore structure	1019:1053	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	3	10	theme	@	432:432	arg1	MgS					433:435	The newly fabricated Alg@MgS	408:435	The newly fabricated Alg@MgS	408:435	The newly fabricated Alg@MgS was characterized by XRD, SEM, and FT-IR.					
36181897	7	11	theme	pore	1040:1043	arg1	structure					1045:1053	controlled nanosized pore structure	1019:1053	controlled nanosized pore structure	1019:1053	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	3	12	theme	fabricated	418:427	arg1	MgS					433:435	The newly fabricated Alg@MgS	408:435	The newly fabricated Alg@MgS	408:435	The newly fabricated Alg@MgS was characterized by XRD, SEM, and FT-IR.					
36181897	7	13	theme	adsorption	1136:1145	arg1	84.7 mg/g					1169:1177	84.7 mg/g	1169:1177	84.7 mg/g	1169:1177	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	7	13	theme	adsorption	1136:1145	arg1	capacity					1147:1154	the highest adsorption capacity	1124:1154	the highest adsorption capacity for Pb2+	1124:1163	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	8	14	theme	sorption	1184:1191	arg1	mechanism					1193:1201	The sorption mechanism	1180:1201	The sorption mechanism	1180:1201	The sorption mechanism was explored by employing several isotherms.					
36181897	1	15	theme	caged	153:157	arg1	nanoparticles					177:189	alginate (Alg) caged magnesium sulfide nanoparticles	138:189	alginate (Alg) caged magnesium sulfide nanoparticles (MgS)	138:195	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	1	15	theme	caged	153:157	arg1	MgS					192:194	MgS	192:194	MgS	192:194	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	1	16	theme	Alg	148:150	arg1	nanoparticles					177:189	alginate (Alg) caged magnesium sulfide nanoparticles	138:189	alginate (Alg) caged magnesium sulfide nanoparticles (MgS)	138:195	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	1	16	theme	Alg	148:150	arg1	MgS					192:194	MgS	192:194	MgS	192:194	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	0	17	from	wastewater	26:35	arg1	Removal					0:6	Removal	0:6	Removal of lead ions from wastewater using magnesium sulfide	0:59	Removal of lead ions from wastewater using magnesium sulfide nanoparticles caged alginate microbeads.					
36181897	4	18	theme	co-ions	734:740	arg1	time					708:711	equilibration time	694:711	equilibration time	694:711	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	18	theme	co-ions	734:740	arg1	concentration					628:640	initial concentration	620:640	initial concentration of lead ions	620:653	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	18	theme	co-ions	734:740	arg1	dosage					664:669	Alg/MgS dosage	656:669	Alg/MgS dosage	656:669	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	18	theme	co-ions	734:740	arg1	temperature					681:691	reaction temperature	672:691	reaction temperature	672:691	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	18	theme	co-ions	734:740	arg1	pH					616:617	pH	616:617	pH	616:617	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	18	theme	co-ions	734:740	arg1	presence					722:729	the presence	718:729	the presence of co-ions	718:740	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	19	theme	reaction	672:679	arg1	temperature					681:691	reaction temperature	672:691	reaction temperature	672:691	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	2	20	theme	hydrogel	322:329	arg1	microbeads					331:340	Alg@MgS hydrogel microbeads	314:340	Alg@MgS hydrogel microbeads	314:340	The MgS nanoparticles were synthesized at low temperatures, and Alg@MgS hydrogel microbeads were made by the ion exchange process of the composite materials.					
36181897	7	21	theme	controlled	1019:1028	arg1	structure					1045:1053	controlled nanosized pore structure	1019:1053	controlled nanosized pore structure	1019:1053	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	10	22	theme	adsorption	1388:1397	arg1	kinetics					1399:1406	the adsorption kinetics	1384:1406	the adsorption kinetics	1384:1406	The pseudo-second-order model adequately described the adsorption kinetics.					
36181897	4	23	theme	ions	650:653	arg1	time					708:711	equilibration time	694:711	equilibration time	694:711	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	23	theme	ions	650:653	arg1	concentration					628:640	initial concentration	620:640	initial concentration of lead ions	620:653	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	23	theme	ions	650:653	arg1	dosage					664:669	Alg/MgS dosage	656:669	Alg/MgS dosage	656:669	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	23	theme	ions	650:653	arg1	temperature					681:691	reaction temperature	672:691	reaction temperature	672:691	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	23	theme	ions	650:653	arg1	pH					616:617	pH	616:617	pH	616:617	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	23	theme	ions	650:653	arg1	presence					722:729	the presence	718:729	the presence of co-ions	718:740	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	2	24	theme	MgS	318:320	arg1	microbeads					331:340	Alg@MgS hydrogel microbeads	314:340	Alg@MgS hydrogel microbeads	314:340	The MgS nanoparticles were synthesized at low temperatures, and Alg@MgS hydrogel microbeads were made by the ion exchange process of the composite materials.					
36181897	7	25	theme	nanosized	1030:1038	arg1	structure					1045:1053	controlled nanosized pore structure	1019:1053	controlled nanosized pore structure	1019:1053	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	2	26	theme	MgS	254:256	arg1	nanoparticles					258:270	The MgS nanoparticles	250:270	The MgS nanoparticles	250:270	The MgS nanoparticles were synthesized at low temperatures, and Alg@MgS hydrogel microbeads were made by the ion exchange process of the composite materials.					
36181897	1	27	theme	magnesium	159:167	arg1	nanoparticles					177:189	alginate (Alg) caged magnesium sulfide nanoparticles	138:189	alginate (Alg) caged magnesium sulfide nanoparticles (MgS)	138:195	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	1	27	theme	magnesium	159:167	arg1	MgS					192:194	MgS	192:194	MgS	192:194	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	2	28	theme	exchange	363:370	arg1	process					372:378	the ion exchange process	355:378	the ion exchange process of the composite materials	355:405	The MgS nanoparticles were synthesized at low temperatures, and Alg@MgS hydrogel microbeads were made by the ion exchange process of the composite materials.					
36181897	1	29	theme	Pb2+	238:241	arg1	ions					243:246	Pb2+ ions	238:246	Pb2+ ions	238:246	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	1	29	theme	Pb2+	238:241	arg1	ions					232:235	lead ions	227:235	lead ions (Pb2+ ions)	227:247	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	10	30	theme	pseudo-second-order	1337:1355	arg1	model					1357:1361	The pseudo-second-order model	1333:1361	The pseudo-second-order model	1333:1361	The pseudo-second-order model adequately described the adsorption kinetics.					
36181897	4	31	theme	Alg/MgS	656:662	arg1	dosage					664:669	Alg/MgS dosage	656:669	Alg/MgS dosage	656:669	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	1	32	theme	sulfide	169:175	arg1	nanoparticles					177:189	alginate (Alg) caged magnesium sulfide nanoparticles	138:189	alginate (Alg) caged magnesium sulfide nanoparticles (MgS)	138:195	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	1	32	theme	sulfide	169:175	arg1	MgS					192:194	MgS	192:194	MgS	192:194	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	2	33	theme	ion	359:361	arg1	process					372:378	the ion exchange process	355:378	the ion exchange process of the composite materials	355:405	The MgS nanoparticles were synthesized at low temperatures, and Alg@MgS hydrogel microbeads were made by the ion exchange process of the composite materials.					
36181897	7	34	theme	MgS	1090:1092	arg1	equilibrium					1071:1081	the adsorption equilibrium	1056:1081	the adsorption equilibrium of Alg@MgS	1056:1092	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	5	35	theme	maximum	780:786	arg1	amount					788:793	the maximum amount	776:793	the maximum amount of Pb2+ ions	776:806	This is accomplished by removing the maximum amount of Pb2+ ions.					
36181897	5	35	theme	maximum	780:786	arg1	ions					803:806	Pb2+ ions	798:806	Pb2+ ions	798:806	This is accomplished by removing the maximum amount of Pb2+ ions.					
36181897	7	36	theme	excellent	989:997	arg1	chelation					1005:1013	excellent metal chelation	989:1013	excellent metal chelation	989:1013	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	4	37	theme	physicochemical	578:592	arg1	time					708:711	equilibration time	694:711	equilibration time	694:711	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	37	theme	physicochemical	578:592	arg1	concentration					628:640	initial concentration	620:640	initial concentration of lead ions	620:653	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	37	theme	physicochemical	578:592	arg1	dosage					664:669	Alg/MgS dosage	656:669	Alg/MgS dosage	656:669	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	37	theme	physicochemical	578:592	arg1	parameters					594:603	several physicochemical parameters	570:603	several physicochemical parameters	570:603	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	37	theme	physicochemical	578:592	arg1	temperature					681:691	reaction temperature	672:691	reaction temperature	672:691	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	37	theme	physicochemical	578:592	arg1	pH					616:617	pH	616:617	pH	616:617	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	37	theme	physicochemical	578:592	arg1	presence					722:729	the presence	718:729	the presence of co-ions	718:740	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	6	38	used	utilized	833:840	arg2	adsorbent					823:831	adsorbent	823:831	adsorbent	823:831	Moreover, the adsorbent utilized more than six times with a substantial amount (not less than 60%) of Pb2+ ions was eliminated.					
36181897	8	39	theme	several	1229:1235	arg1	isotherms					1237:1245	several isotherms	1229:1245	several isotherms	1229:1245	The sorption mechanism was explored by employing several isotherms.					
36181897	2	40	theme	@	317:317	arg1	microbeads					331:340	Alg@MgS hydrogel microbeads	314:340	Alg@MgS hydrogel microbeads	314:340	The MgS nanoparticles were synthesized at low temperatures, and Alg@MgS hydrogel microbeads were made by the ion exchange process of the composite materials.					
36181897	4	41	theme	ions	552:555	arg1	removal					536:542	the maximum removal	524:542	the maximum removal of Pb2+ ions	524:555	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	2	42	theme	materials	397:405	arg1	process					372:378	the ion exchange process	355:378	the ion exchange process of the composite materials	355:405	The MgS nanoparticles were synthesized at low temperatures, and Alg@MgS hydrogel microbeads were made by the ion exchange process of the composite materials.					
36181897	7	43	theme	metal	999:1003	arg1	chelation					1005:1013	excellent metal chelation	989:1013	excellent metal chelation	989:1013	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	0	44	theme	ions	16:19	arg1	Removal					0:6	Removal	0:6	Removal of lead ions from wastewater using magnesium sulfide	0:59	Removal of lead ions from wastewater using magnesium sulfide nanoparticles caged alginate microbeads.					
36181897	4	45	theme	Pb2+	547:550	arg1	ions					552:555	Pb2+ ions	547:555	Pb2+ ions	547:555	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	0	46	theme	magnesium	43:51	arg1	sulfide					53:59	magnesium sulfide	43:59	magnesium sulfide	43:59	Removal of lead ions from wastewater using magnesium sulfide nanoparticles caged alginate microbeads.					
36181897	9	47	theme	Freundlich	1270:1279	arg1	model					1281:1285	the Freundlich model	1266:1285	the Freundlich model	1266:1285	It was found that the Freundlich model fits the adsorption process quite accurately.					
36181897	7	48	theme	Alg	1086:1088	arg1	MgS					1090:1092	Alg@MgS	1086:1092	Alg@MgS	1086:1092	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	2	49	theme	low	292:294	arg1	temperatures					296:307	low temperatures	292:307	low temperatures	292:307	The MgS nanoparticles were synthesized at low temperatures, and Alg@MgS hydrogel microbeads were made by the ion exchange process of the composite materials.					
36181897	1	50	used	used	213:216	arg2	microbeads					197:206	an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads	117:206	an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads	117:206	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	9	51	theme	adsorption	1296:1305	arg1	process					1307:1313	the adsorption process	1292:1313	the adsorption process	1292:1313	It was found that the Freundlich model fits the adsorption process quite accurately.					
36181897	3	52	theme	Alg	429:431	arg1	MgS					433:435	The newly fabricated Alg@MgS	408:435	The newly fabricated Alg@MgS	408:435	The newly fabricated Alg@MgS was characterized by XRD, SEM, and FT-IR.					
36181897	4	53	theme	initial	620:626	arg1	concentration					628:640	initial concentration	620:640	initial concentration of lead ions	620:653	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	54	theme	several	570:576	arg1	time					708:711	equilibration time	694:711	equilibration time	694:711	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	54	theme	several	570:576	arg1	concentration					628:640	initial concentration	620:640	initial concentration of lead ions	620:653	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	54	theme	several	570:576	arg1	dosage					664:669	Alg/MgS dosage	656:669	Alg/MgS dosage	656:669	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	54	theme	several	570:576	arg1	parameters					594:603	several physicochemical parameters	570:603	several physicochemical parameters	570:603	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	54	theme	several	570:576	arg1	temperature					681:691	reaction temperature	672:691	reaction temperature	672:691	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	54	theme	several	570:576	arg1	pH					616:617	pH	616:617	pH	616:617	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	54	theme	several	570:576	arg1	presence					722:729	the presence	718:729	the presence of co-ions	718:740	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	4	55	theme	equilibration	694:706	arg1	time					708:711	equilibration time	694:711	equilibration time	694:711	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	6	56	theme	substantial	869:879	arg1	amount					881:886	a substantial amount	867:886	a substantial amount (not less than 60%) of Pb2+ ions	867:919	Moreover, the adsorbent utilized more than six times with a substantial amount (not less than 60%) of Pb2+ ions was eliminated.					
36181897	6	56	theme	substantial	869:879	arg1	%					905:905	not less than 60%	889:905	not less than 60%	889:905	Moreover, the adsorbent utilized more than six times with a substantial amount (not less than 60%) of Pb2+ ions was eliminated.					
36181897	6	56	theme	substantial	869:879	arg1	ions					916:919	Pb2+ ions	911:919	Pb2+ ions	911:919	Moreover, the adsorbent utilized more than six times with a substantial amount (not less than 60%) of Pb2+ ions was eliminated.					
36181897	5	57	theme	ions	803:806	arg1	amount					788:793	the maximum amount	776:793	the maximum amount of Pb2+ ions	776:806	This is accomplished by removing the maximum amount of Pb2+ ions.					
36181897	5	57	theme	ions	803:806	arg1	ions					803:806	Pb2+ ions	798:806	Pb2+ ions	798:806	This is accomplished by removing the maximum amount of Pb2+ ions.					
36181897	6	58	theme	ions	916:919	arg1	amount					881:886	a substantial amount	867:886	a substantial amount (not less than 60%) of Pb2+ ions	867:919	Moreover, the adsorbent utilized more than six times with a substantial amount (not less than 60%) of Pb2+ ions was eliminated.					
36181897	6	58	theme	ions	916:919	arg1	%					905:905	not less than 60%	889:905	not less than 60%	889:905	Moreover, the adsorbent utilized more than six times with a substantial amount (not less than 60%) of Pb2+ ions was eliminated.					
36181897	6	58	theme	ions	916:919	arg1	ions					916:919	Pb2+ ions	911:919	Pb2+ ions	911:919	Moreover, the adsorbent utilized more than six times with a substantial amount (not less than 60%) of Pb2+ ions was eliminated.					
36181897	1	59	theme	adsorbent	120:128	arg1	microbeads					197:206	an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads	117:206	an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads	117:206	In this study, an adsorbent made of alginate (Alg) caged magnesium sulfide nanoparticles (MgS) microbeads were used to treat lead ions (Pb2+ ions).					
36181897	6	60	theme	Pb2+	911:914	arg1	ions					916:919	Pb2+ ions	911:919	Pb2+ ions	911:919	Moreover, the adsorbent utilized more than six times with a substantial amount (not less than 60%) of Pb2+ ions was eliminated.					
36181897	2	61	theme	Alg	314:316	arg1	microbeads					331:340	Alg@MgS hydrogel microbeads	314:340	Alg@MgS hydrogel microbeads	314:340	The MgS nanoparticles were synthesized at low temperatures, and Alg@MgS hydrogel microbeads were made by the ion exchange process of the composite materials.					
36181897	7	62	theme	highest	1128:1134	arg1	84.7 mg/g					1169:1177	84.7 mg/g	1169:1177	84.7 mg/g	1169:1177	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	7	62	theme	highest	1128:1134	arg1	capacity					1147:1154	the highest adsorption capacity	1124:1154	the highest adsorption capacity for Pb2+	1124:1163	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
36181897	4	63	theme	maximum	528:534	arg1	removal					536:542	the maximum removal	524:542	the maximum removal of Pb2+ ions	524:555	The adsorption conditions were optimized for the maximum removal of Pb2+ ions by adjusting several physicochemical parameters, including pH, initial concentration of lead ions, Alg/MgS dosage, reaction temperature, equilibration time, and the presence of co-ions.					
36181897	2	64	theme	composite	387:395	arg1	materials					397:405	the composite materials	383:405	the composite materials	383:405	The MgS nanoparticles were synthesized at low temperatures, and Alg@MgS hydrogel microbeads were made by the ion exchange process of the composite materials.					
36181897	0	65	theme	lead	11:14	arg1	ions					16:19	lead ions	11:19	lead ions	11:19	Removal of lead ions from wastewater using magnesium sulfide nanoparticles caged alginate microbeads.					
36181897	7	66	theme	@	1089:1089	arg1	MgS					1090:1092	Alg@MgS	1086:1092	Alg@MgS	1086:1092	Considering the ability of sodium alginate (SA) for excellent metal chelation and controlled nanosized pore structure, the adsorption equilibrium of Alg@MgS can be reached in 60 min, and the highest adsorption capacity for Pb2+ was 84.7 mg/g.					
35722898	11	0	theme	antioxidant	1828:1838	arg1	activity					1840:1847	the antioxidant activity	1824:1847	the antioxidant activity of breakfast cereals	1824:1868	Honey addition lowers the loss of phenolic content during extrusion and improves the antioxidant activity of breakfast cereals.					
35722898	10	1	theme	cereals	1612:1618	arg1	content					1591:1597	The total phenolic content	1572:1597	The total phenolic content of breakfast cereals	1572:1618	The total phenolic content of breakfast cereals decreased, and antioxidant activity and hydroxymethylfurfural content increased upon extrusion at the higher temperatures.					
35722898	5	2	from	CCRD	927:930	arg1	experiments					910:920	The selected experiments	897:920	The selected experiments from CCRD	897:930	The selected experiments from CCRD were considered for studying the bioactive properties, and the effect of ET and honey level on bioactive properties was determined.					
35722898	1	3	theme	whole-grain	295:305	arg1	cereals					328:334	whole-grain corn flour breakfast cereals	295:334	whole-grain corn flour breakfast cereals	295:334	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	9	4	theme	optimum	1514:1520	arg1	cereals					1540:1546	optimum quality breakfast cereals	1514:1546	optimum quality breakfast cereals	1514:1546	The whole-grain corn flour added with 10.22% honey and extruded at 16.06% FM, and 138.07°C ET produced optimum quality breakfast cereals with 0.70 desirability.					
35722898	6	5	theme	study	1083:1087	arg1	results					1068:1074	The results	1064:1074	The results of the study	1064:1087	The results of the study indicated that FM and honey adversely affected the SER, BD, and textural hardness, while ET augmented these properties of breakfast cereals.					
35722898	2	6	theme	absorption	569:578	arg1	WAI					587:589	WAI	587:589	WAI	587:589	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	6	theme	absorption	569:578	arg1	index					580:584	water absorption index	563:584	water absorption index (WAI)	563:590	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	7	theme	central	341:347	arg1	CCRD					377:380	CCRD	377:380	CCRD	377:380	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	7	theme	central	341:347	arg1	design					369:374	The central composite rotatable design	337:374	The central composite rotatable design (CCRD)	337:381	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	0	8	theme	cereals	129:135	arg1	properties					76:85	techno-functional, textural and bioactive properties	34:85	techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals	34:135	Effect of extrusion processing on techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals sweetened with honey.					
35722898	7	9	theme	honey	1268:1272	arg1	level					1274:1278	honey level	1268:1278	honey level	1268:1278	The WAI decreased with an increase in honey level and ET during extrusion.					
35722898	3	10	theme	whole-grain	698:708	arg1	flour					715:719	honey and whole-grain corn flour	688:719	flour	715:719	The feed containing honey and whole-grain corn flour was extruded through a twin-screw extruder according to CCRD.					
35722898	6	11	theme	breakfast	1211:1219	arg1	cereals					1221:1227	breakfast cereals	1211:1227	breakfast cereals	1211:1227	The results of the study indicated that FM and honey adversely affected the SER, BD, and textural hardness, while ET augmented these properties of breakfast cereals.					
35722898	11	12	theme	breakfast	1852:1860	arg1	cereals					1862:1868	breakfast cereals	1852:1868	breakfast cereals	1852:1868	Honey addition lowers the loss of phenolic content during extrusion and improves the antioxidant activity of breakfast cereals.					
35722898	1	13	theme	processing	224:233	arg1	parameters					235:244	honey and extrusion processing parameters	204:244	honey and extrusion processing parameters	204:244	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	4	14	theme	appropriate	808:818	arg1	models					831:836	the appropriate regression models	804:836	the appropriate regression models based on model significance and insignificant lack of fit	804:894	The data were fit to the appropriate regression models based on model significance and insignificant lack of fit.					
35722898	3	15	theme	honey	688:692	arg1	flour					715:719	honey and whole-grain corn flour	688:719	flour	715:719	The feed containing honey and whole-grain corn flour was extruded through a twin-screw extruder according to CCRD.					
35722898	5	16	theme	bioactive	965:973	arg1	properties					975:984	the bioactive properties	961:984	the bioactive properties	961:984	The selected experiments from CCRD were considered for studying the bioactive properties, and the effect of ET and honey level on bioactive properties was determined.					
35722898	1	17	from	effect	194:199	arg1	properties					281:290	techno-functional and bioactive properties	249:290	techno-functional and bioactive properties of whole-grain corn flour breakfast cereals	249:334	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	2	18	theme	response	648:655	arg1	level					480:484	honey level	474:484	honey level	474:484	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	18	theme	response	648:655	arg1	moisture					427:434	feed moisture	422:434	feed moisture (FM)	422:439	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	18	theme	response	648:655	arg1	temperature					452:462	extrusion temperature	442:462	extrusion temperature (ET)	442:467	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	18	theme	response	648:655	arg1	variables					657:665	response variables	648:665	response variables	648:665	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	5	19	theme	bioactive	1027:1035	arg1	properties					1037:1046	bioactive properties	1027:1046	bioactive properties	1027:1046	The selected experiments from CCRD were considered for studying the bioactive properties, and the effect of ET and honey level on bioactive properties was determined.					
35722898	0	20	theme	extrusion	10:18	arg1	processing					20:29	extrusion processing	10:29	extrusion processing	10:29	Effect of extrusion processing on techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals sweetened with honey.					
35722898	2	21	theme	honey	474:478	arg1	level					480:484	honey level	474:484	honey level	474:484	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	21	theme	honey	474:478	arg1	variables					497:505	process variables	489:505	process variables	489:505	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	21	theme	honey	474:478	arg1	temperature					452:462	extrusion temperature	442:462	extrusion temperature (ET)	442:467	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	21	theme	honey	474:478	arg1	moisture					427:434	feed moisture	422:434	feed moisture (FM)	422:439	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	21	theme	honey	474:478	arg1	variables					657:665	response variables	648:665	response variables	648:665	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	22	theme	sectional	511:519	arg1	SER					538:540	SER	538:540	SER	538:540	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	22	theme	sectional	511:519	arg1	ratio					531:535	sectional expansion ratio	511:535	sectional expansion ratio (SER)	511:541	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	0	23	from	Effect	0:5	arg1	properties					76:85	techno-functional, textural and bioactive properties	34:85	techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals	34:135	Effect of extrusion processing on techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals sweetened with honey.					
35722898	0	24	theme	whole-grain	90:100	arg1	cereals					129:135	whole-grain corn flour-based breakfast cereals	90:135	whole-grain corn flour-based breakfast cereals	90:135	Effect of extrusion processing on techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals sweetened with honey.					
35722898	9	25	theme	whole-grain	1415:1425	arg1	flour					1432:1436	The whole-grain corn flour	1411:1436	The whole-grain corn flour	1411:1436	The whole-grain corn flour added with 10.22% honey and extruded at 16.06% FM, and 138.07°C ET produced optimum quality breakfast cereals with 0.70 desirability.					
35722898	11	26	theme	content	1786:1792	arg1	loss					1769:1772	the loss	1765:1772	the loss of phenolic content during extrusion	1765:1809	Honey addition lowers the loss of phenolic content during extrusion and improves the antioxidant activity of breakfast cereals.					
35722898	0	27	theme	flour-based	107:117	arg1	cereals					129:135	whole-grain corn flour-based breakfast cereals	90:135	whole-grain corn flour-based breakfast cereals	90:135	Effect of extrusion processing on techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals sweetened with honey.					
35722898	5	28	theme	level	1018:1022	arg1	effect					995:1000	the effect	991:1000	the effect of ET and honey level on bioactive properties	991:1046	The selected experiments from CCRD were considered for studying the bioactive properties, and the effect of ET and honey level on bioactive properties was determined.					
35722898	5	29	theme	selected	901:908	arg1	experiments					910:920	The selected experiments	897:920	The selected experiments from CCRD	897:930	The selected experiments from CCRD were considered for studying the bioactive properties, and the effect of ET and honey level on bioactive properties was determined.					
35722898	8	30	from	temperature	1380:1390	arg1	addition					1321:1328	The incremental addition	1305:1328	The incremental addition of honey in the mix as well extrusion at elevated temperature	1305:1390	The incremental addition of honey in the mix as well extrusion at elevated temperature led to higher WSI.					
35722898	1	31	theme	corn	307:310	arg1	cereals					328:334	whole-grain corn flour breakfast cereals	295:334	whole-grain corn flour breakfast cereals	295:334	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	7	32	from	increase	1256:1263	arg1	level					1274:1278	honey level	1268:1278	honey level	1268:1278	The WAI decreased with an increase in honey level and ET during extrusion.					
35722898	2	33	theme	solubility	599:608	arg1	index					610:614	water solubility index	593:614	water solubility index (WSI)	593:620	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	33	theme	solubility	599:608	arg1	WSI					617:619	WSI	617:619	WSI	617:619	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	3	34	contain	containing	677:686	arg1	feed					672:675	The feed	668:675	The feed containing honey and whole-grain corn flour	668:719	The feed containing honey and whole-grain corn flour was extruded through a twin-screw extruder according to CCRD.					
35722898	3	34	contain	containing	677:686	arg2	flour					715:719	honey and whole-grain corn flour	688:719	flour	715:719	The feed containing honey and whole-grain corn flour was extruded through a twin-screw extruder according to CCRD.					
35722898	9	35	dep	138.07°C	1493:1500	arg1	ET					1502:1503	138.07°C ET	1493:1503	138.07°C ET	1493:1503	The whole-grain corn flour added with 10.22% honey and extruded at 16.06% FM, and 138.07°C ET produced optimum quality breakfast cereals with 0.70 desirability.					
35722898	1	36	theme	breakfast	318:326	arg1	cereals					328:334	whole-grain corn flour breakfast cereals	295:334	whole-grain corn flour breakfast cereals	295:334	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	9	37	theme	%	1454:1454	arg1	honey					1456:1460	10.22% honey	1449:1460	10.22% honey	1449:1460	The whole-grain corn flour added with 10.22% honey and extruded at 16.06% FM, and 138.07°C ET produced optimum quality breakfast cereals with 0.70 desirability.					
35722898	10	38	theme	higher	1722:1727	arg1	temperatures					1729:1740	the higher temperatures	1718:1740	the higher temperatures	1718:1740	The total phenolic content of breakfast cereals decreased, and antioxidant activity and hydroxymethylfurfural content increased upon extrusion at the higher temperatures.					
35722898	1	39	theme	parameters	235:244	arg1	effect					194:199	the effect	190:199	the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals	190:334	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	9	40	theme	%	1483:1483	arg1	FM					1485:1486	16.06% FM	1478:1486	16.06% FM	1478:1486	The whole-grain corn flour added with 10.22% honey and extruded at 16.06% FM, and 138.07°C ET produced optimum quality breakfast cereals with 0.70 desirability.					
35722898	2	41	theme	feed	422:425	arg1	variables					497:505	process variables	489:505	process variables	489:505	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	41	theme	feed	422:425	arg1	variables					657:665	response variables	648:665	response variables	648:665	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	41	theme	feed	422:425	arg1	temperature					452:462	extrusion temperature	442:462	extrusion temperature (ET)	442:467	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	41	theme	feed	422:425	arg1	level					480:484	honey level	474:484	honey level	474:484	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	41	theme	feed	422:425	arg1	moisture					427:434	feed moisture	422:434	feed moisture (FM)	422:439	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	41	theme	feed	422:425	arg1	FM					437:438	FM	437:438	FM	437:438	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	1	42	theme	techno-functional	249:265	arg1	properties					281:290	techno-functional and bioactive properties	249:290	techno-functional and bioactive properties of whole-grain corn flour breakfast cereals	249:334	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	10	43	theme	total	1576:1580	arg1	content					1591:1597	The total phenolic content	1572:1597	The total phenolic content of breakfast cereals	1572:1618	The total phenolic content of breakfast cereals decreased, and antioxidant activity and hydroxymethylfurfural content increased upon extrusion at the higher temperatures.					
35722898	1	44	theme	bioactive	271:279	arg1	properties					281:290	techno-functional and bioactive properties	249:290	techno-functional and bioactive properties of whole-grain corn flour breakfast cereals	249:334	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	9	45	theme	breakfast	1530:1538	arg1	cereals					1540:1546	optimum quality breakfast cereals	1514:1546	optimum quality breakfast cereals	1514:1546	The whole-grain corn flour added with 10.22% honey and extruded at 16.06% FM, and 138.07°C ET produced optimum quality breakfast cereals with 0.70 desirability.					
35722898	6	46	theme	textural	1153:1160	arg1	hardness					1162:1169	textural hardness	1153:1169	textural hardness	1153:1169	The results of the study indicated that FM and honey adversely affected the SER, BD, and textural hardness, while ET augmented these properties of breakfast cereals.					
35722898	10	47	theme	breakfast	1602:1610	arg1	cereals					1612:1618	breakfast cereals	1602:1618	breakfast cereals	1602:1618	The total phenolic content of breakfast cereals decreased, and antioxidant activity and hydroxymethylfurfural content increased upon extrusion at the higher temperatures.					
35722898	9	48	theme	quality	1522:1528	arg1	cereals					1540:1546	optimum quality breakfast cereals	1514:1546	optimum quality breakfast cereals	1514:1546	The whole-grain corn flour added with 10.22% honey and extruded at 16.06% FM, and 138.07°C ET produced optimum quality breakfast cereals with 0.70 desirability.					
35722898	2	49	theme	composite	349:357	arg1	CCRD					377:380	CCRD	377:380	CCRD	377:380	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	49	theme	composite	349:357	arg1	design					369:374	The central composite rotatable design	337:374	The central composite rotatable design (CCRD)	337:381	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	50	theme	water	563:567	arg1	WAI					587:589	WAI	587:589	WAI	587:589	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	50	theme	water	563:567	arg1	index					580:584	water absorption index	563:584	water absorption index (WAI)	563:590	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	1	51	theme	honey	204:208	arg1	parameters					235:244	honey and extrusion processing parameters	204:244	honey and extrusion processing parameters	204:244	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	10	52	theme	antioxidant	1635:1645	arg1	activity					1647:1654	antioxidant activity	1635:1654	antioxidant activity	1635:1654	The total phenolic content of breakfast cereals decreased, and antioxidant activity and hydroxymethylfurfural content increased upon extrusion at the higher temperatures.					
35722898	8	53	theme	honey	1333:1337	arg1	extrusion					1358:1366	honey in the mix as well extrusion	1333:1366	honey in the mix as well extrusion	1333:1366	The incremental addition of honey in the mix as well extrusion at elevated temperature led to higher WSI.					
35722898	6	54	theme	cereals	1221:1227	arg1	properties					1197:1206	these properties	1191:1206	these properties of breakfast cereals	1191:1227	The results of the study indicated that FM and honey adversely affected the SER, BD, and textural hardness, while ET augmented these properties of breakfast cereals.					
35722898	1	55	theme	extrusion	214:222	arg1	parameters					235:244	honey and extrusion processing parameters	204:244	honey and extrusion processing parameters	204:244	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	2	56	theme	expansion	521:529	arg1	SER					538:540	SER	538:540	SER	538:540	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	56	theme	expansion	521:529	arg1	ratio					531:535	sectional expansion ratio	511:535	sectional expansion ratio (SER)	511:541	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	57	theme	textural	627:634	arg1	hardness					636:643	textural hardness	627:643	textural hardness	627:643	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	4	58	theme	insignificant	870:882	arg1	lack					884:887	insignificant lack	870:887	insignificant lack	870:887	The data were fit to the appropriate regression models based on model significance and insignificant lack of fit.					
35722898	5	59	theme	ET	1005:1006	arg1	effect					995:1000	the effect	991:1000	the effect of ET and honey level on bioactive properties	991:1046	The selected experiments from CCRD were considered for studying the bioactive properties, and the effect of ET and honey level on bioactive properties was determined.					
35722898	10	60	theme	hydroxymethylfurfural	1660:1680	arg1	content					1682:1688	hydroxymethylfurfural content	1660:1688	hydroxymethylfurfural content	1660:1688	The total phenolic content of breakfast cereals decreased, and antioxidant activity and hydroxymethylfurfural content increased upon extrusion at the higher temperatures.					
35722898	2	61	theme	bulk	544:547	arg1	BD					558:559	BD	558:559	BD	558:559	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	61	theme	bulk	544:547	arg1	density					549:555	bulk density	544:555	bulk density (BD)	544:560	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	4	62	theme	regression	820:829	arg1	models					831:836	the appropriate regression models	804:836	the appropriate regression models based on model significance and insignificant lack of fit	804:894	The data were fit to the appropriate regression models based on model significance and insignificant lack of fit.					
35722898	8	63	theme	extrusion	1358:1366	arg1	addition					1321:1328	The incremental addition	1305:1328	The incremental addition of honey in the mix as well extrusion at elevated temperature	1305:1390	The incremental addition of honey in the mix as well extrusion at elevated temperature led to higher WSI.					
35722898	0	64	theme	bioactive	66:74	arg1	properties					76:85	techno-functional, textural and bioactive properties	34:85	techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals	34:135	Effect of extrusion processing on techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals sweetened with honey.					
35722898	11	65	theme	cereals	1862:1868	arg1	activity					1840:1847	the antioxidant activity	1824:1847	the antioxidant activity of breakfast cereals	1824:1868	Honey addition lowers the loss of phenolic content during extrusion and improves the antioxidant activity of breakfast cereals.					
35722898	4	66	theme	fit	892:894	arg1	lack					884:887	insignificant lack	870:887	insignificant lack	870:887	The data were fit to the appropriate regression models based on model significance and insignificant lack of fit.					
35722898	4	66	theme	fit	892:894	arg1	significance					853:864	model significance	847:864	model significance	847:864	The data were fit to the appropriate regression models based on model significance and insignificant lack of fit.					
35722898	8	67	theme	elevated	1371:1378	arg1	temperature					1380:1390	elevated temperature	1371:1390	elevated temperature	1371:1390	The incremental addition of honey in the mix as well extrusion at elevated temperature led to higher WSI.					
35722898	8	68	from	honey	1333:1337	arg1	mix					1346:1348	the mix	1342:1348	the mix	1342:1348	The incremental addition of honey in the mix as well extrusion at elevated temperature led to higher WSI.					
35722898	1	69	theme	present	163:169	arg1	study					171:175	The present study	159:175	The present study	159:175	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	2	70	theme	water	593:597	arg1	index					610:614	water solubility index	593:614	water solubility index (WSI)	593:620	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	70	theme	water	593:597	arg1	WSI					617:619	WSI	617:619	WSI	617:619	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	0	71	theme	corn	102:105	arg1	cereals					129:135	whole-grain corn flour-based breakfast cereals	90:135	whole-grain corn flour-based breakfast cereals	90:135	Effect of extrusion processing on techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals sweetened with honey.					
35722898	8	72	theme	higher	1399:1404	arg1	WSI					1406:1408	higher WSI	1399:1408	higher WSI	1399:1408	The incremental addition of honey in the mix as well extrusion at elevated temperature led to higher WSI.					
35722898	2	73	used	used	387:390	arg2	CCRD					377:380	CCRD	377:380	CCRD	377:380	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	73	used	used	387:390	arg2	design					369:374	The central composite rotatable design	337:374	The central composite rotatable design (CCRD)	337:381	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	3	74	theme	twin-screw	744:753	arg1	extruder					755:762	a twin-screw extruder	742:762	a twin-screw extruder according to CCRD	742:780	The feed containing honey and whole-grain corn flour was extruded through a twin-screw extruder according to CCRD.					
35722898	0	75	theme	breakfast	119:127	arg1	cereals					129:135	whole-grain corn flour-based breakfast cereals	90:135	whole-grain corn flour-based breakfast cereals	90:135	Effect of extrusion processing on techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals sweetened with honey.					
35722898	5	76	theme	honey	1012:1016	arg1	level					1018:1022	honey level	1012:1022	honey level	1012:1022	The selected experiments from CCRD were considered for studying the bioactive properties, and the effect of ET and honey level on bioactive properties was determined.					
35722898	9	77	theme	corn	1427:1430	arg1	flour					1432:1436	The whole-grain corn flour	1411:1436	The whole-grain corn flour	1411:1436	The whole-grain corn flour added with 10.22% honey and extruded at 16.06% FM, and 138.07°C ET produced optimum quality breakfast cereals with 0.70 desirability.					
35722898	2	78	theme	process	489:495	arg1	level					480:484	honey level	474:484	honey level	474:484	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	78	theme	process	489:495	arg1	variables					497:505	process variables	489:505	process variables	489:505	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	78	theme	process	489:495	arg1	temperature					452:462	extrusion temperature	442:462	extrusion temperature (ET)	442:467	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	78	theme	process	489:495	arg1	moisture					427:434	feed moisture	422:434	feed moisture (FM)	422:439	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	8	79	from	mix	1346:1348	arg1	honey					1333:1337	honey	1333:1337	honey	1333:1337	The incremental addition of honey in the mix as well extrusion at elevated temperature led to higher WSI.					
35722898	2	80	dep	used	387:390	arg1	using					416:420	using	416:420	using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables	416:665	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	81	dep	temperature	452:462	arg1	ET					465:466	ET	465:466	ET	465:466	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	82	theme	extrusion	442:450	arg1	level					480:484	honey level	474:484	honey level	474:484	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	82	theme	extrusion	442:450	arg1	variables					497:505	process variables	489:505	process variables	489:505	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	82	theme	extrusion	442:450	arg1	temperature					452:462	extrusion temperature	442:462	extrusion temperature (ET)	442:467	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	82	theme	extrusion	442:450	arg1	moisture					427:434	feed moisture	422:434	feed moisture (FM)	422:439	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	82	theme	extrusion	442:450	arg1	variables					657:665	response variables	648:665	response variables	648:665	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	11	83	theme	phenolic	1777:1784	arg1	content					1786:1792	phenolic content	1777:1792	phenolic content	1777:1792	Honey addition lowers the loss of phenolic content during extrusion and improves the antioxidant activity of breakfast cereals.					
35722898	0	84	theme	processing	20:29	arg1	Effect					0:5	Effect	0:5	Effect of extrusion processing on techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals	0:135	Effect of extrusion processing on techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals sweetened with honey.					
35722898	1	85	theme	flour	312:316	arg1	cereals					328:334	whole-grain corn flour breakfast cereals	295:334	whole-grain corn flour breakfast cereals	295:334	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	9	86	theme	10.22	1449:1453	arg1	%					1454:1454	%	1454:1454	%	1454:1454	The whole-grain corn flour added with 10.22% honey and extruded at 16.06% FM, and 138.07°C ET produced optimum quality breakfast cereals with 0.70 desirability.					
35722898	0	87	theme	techno-functional	34:50	arg1	properties					76:85	techno-functional, textural and bioactive properties	34:85	techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals	34:135	Effect of extrusion processing on techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals sweetened with honey.					
35722898	1	88	theme	cereals	328:334	arg1	properties					281:290	techno-functional and bioactive properties	249:290	techno-functional and bioactive properties of whole-grain corn flour breakfast cereals	249:334	The present study investigated the effect of honey and extrusion processing parameters on techno-functional and bioactive properties of whole-grain corn flour breakfast cereals.					
35722898	5	89	from	effect	995:1000	arg1	properties					1037:1046	bioactive properties	1027:1046	bioactive properties	1027:1046	The selected experiments from CCRD were considered for studying the bioactive properties, and the effect of ET and honey level on bioactive properties was determined.					
35722898	0	90	theme	textural	53:60	arg1	properties					76:85	techno-functional, textural and bioactive properties	34:85	techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals	34:135	Effect of extrusion processing on techno-functional, textural and bioactive properties of whole-grain corn flour-based breakfast cereals sweetened with honey.					
35722898	4	91	theme	model	847:851	arg1	significance					853:864	model significance	847:864	model significance	847:864	The data were fit to the appropriate regression models based on model significance and insignificant lack of fit.					
35722898	11	92	theme	Honey	1743:1747	arg1	addition					1749:1756	Honey addition	1743:1756	Honey addition	1743:1756	Honey addition lowers the loss of phenolic content during extrusion and improves the antioxidant activity of breakfast cereals.					
35722898	9	93	theme	16.06	1478:1482	arg1	%					1483:1483	%	1483:1483	%	1483:1483	The whole-grain corn flour added with 10.22% honey and extruded at 16.06% FM, and 138.07°C ET produced optimum quality breakfast cereals with 0.70 desirability.					
35722898	2	94	theme	rotatable	359:367	arg1	CCRD					377:380	CCRD	377:380	CCRD	377:380	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	2	94	theme	rotatable	359:367	arg1	design					369:374	The central composite rotatable design	337:374	The central composite rotatable design (CCRD)	337:381	The central composite rotatable design (CCRD) was used to plan the experiments using feed moisture (FM), extrusion temperature (ET), and honey level as process variables and sectional expansion ratio (SER), bulk density (BD), water absorption index (WAI), water solubility index (WSI), and textural hardness as response variables.					
35722898	8	95	theme	incremental	1309:1319	arg1	addition					1321:1328	The incremental addition	1305:1328	The incremental addition of honey in the mix as well extrusion at elevated temperature	1305:1390	The incremental addition of honey in the mix as well extrusion at elevated temperature led to higher WSI.					
35722898	10	96	theme	phenolic	1582:1589	arg1	content					1591:1597	The total phenolic content	1572:1597	The total phenolic content of breakfast cereals	1572:1618	The total phenolic content of breakfast cereals decreased, and antioxidant activity and hydroxymethylfurfural content increased upon extrusion at the higher temperatures.					
35722898	3	97	theme	corn	710:713	arg1	flour					715:719	honey and whole-grain corn flour	688:719	flour	715:719	The feed containing honey and whole-grain corn flour was extruded through a twin-screw extruder according to CCRD.					
35710050	0	0	theme	biochar	79:85	arg1	composite					87:95	biochar composite	79:95	biochar composite	79:95	Insights on ball milling enhanced iron magnesium layered double oxides bagasse biochar composite for ciprofloxacin adsorptive removal from water.					
35710050	7	1	theme	rational	1206:1213	arg1	placement					1215:1223	rational placement	1206:1223	rational placement of biowaste and high-efficiency synthesis of biochar	1206:1276	These superiorities illustrated that BM-LDOs-BC was a promising adsorbent and created a new avenue for rational placement of biowaste and high-efficiency synthesis of biochar for antibiotic removal.					
35710050	3	2	theme	BM-LDOs-BC	506:515	arg1	capacity					494:501	The maximum theoretical adsorption capacity	459:501	The maximum theoretical adsorption capacity of BM-LDOs-BC	459:515	The maximum theoretical adsorption capacity of BM-LDOs-BC reached up to 213.1 mg g-1 due to abundant adsorption sites provided by well-developed pores characteristics and enhanced functional groups.					
35710050	4	3	theme	π-π	816:818	arg1	filling					751:757	pore filling	746:757	pore filling	746:757	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	3	theme	π-π	816:818	arg1	mechanisms					845:854	the key mechanisms	837:854	the key mechanisms for CIP adsorptive removal	837:881	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	3	theme	π-π	816:818	arg1	complexation					799:810	complexation	799:810	complexation	799:810	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	3	theme	π-π	816:818	arg1	conjugation					820:830	π-π conjugation	816:830	π-π conjugation	816:830	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	3	theme	π-π	816:818	arg1	interactions					774:785	electrostatic interactions	760:785	electrostatic interactions	760:785	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	3	theme	π-π	816:818	arg1	H-bonding					788:796	H-bonding	788:796	H-bonding	788:796	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	2	4	theme	first	356:360	arg1	time					362:365	the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal	352:456	the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal	352:456	Here, an innovative technique combining Fe-Mg-layered double oxides and ball milling was presented for the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal.					
35710050	6	5	contain	possessed	1070:1078	arg1	BM-LDOs-BC					1059:1068	BM-LDOs-BC	1059:1068	BM-LDOs-BC	1059:1068	Moreover, BM-LDOs-BC possessed excellent reusability.					
35710050	6	5	contain	possessed	1070:1078	arg2	reusability					1090:1100	excellent reusability	1080:1100	excellent reusability	1080:1100	Moreover, BM-LDOs-BC possessed excellent reusability.					
35710050	0	6	from	Insights	0:7	arg1	ball					12:15	ball	12:15	ball milling enhanced iron magnesium layered double oxides	12:69	Insights on ball milling enhanced iron magnesium layered double oxides bagasse biochar composite for ciprofloxacin adsorptive removal from water.					
35710050	2	7	theme	double	303:308	arg1	oxides					310:315	Fe-Mg-layered double oxides	289:315	Fe-Mg-layered double oxides	289:315	Here, an innovative technique combining Fe-Mg-layered double oxides and ball milling was presented for the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal.					
35710050	4	8	theme	data	691:694	arg1	results					662:668	The results	658:668	The results of characterization, data fitting and environmental parameter	658:730	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	3	9	theme	abundant	551:558	arg1	sites					571:575	abundant adsorption sites	551:575	abundant adsorption sites provided by well-developed pores	551:608	The maximum theoretical adsorption capacity of BM-LDOs-BC reached up to 213.1 mg g-1 due to abundant adsorption sites provided by well-developed pores characteristics and enhanced functional groups.					
35710050	2	10	theme	Fe-Mg-layered	289:301	arg1	oxides					310:315	Fe-Mg-layered double oxides	289:315	Fe-Mg-layered double oxides	289:315	Here, an innovative technique combining Fe-Mg-layered double oxides and ball milling was presented for the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal.					
35710050	4	11	theme	characterization	673:688	arg1	results					662:668	The results	658:668	The results of characterization, data fitting and environmental parameter	658:730	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	0	12	theme	adsorptive	115:124	arg1	removal					126:132	ciprofloxacin adsorptive removal	101:132	ciprofloxacin adsorptive removal from water	101:143	Insights on ball milling enhanced iron magnesium layered double oxides bagasse biochar composite for ciprofloxacin adsorptive removal from water.					
35710050	5	13	theme	environmental	989:1001	arg1	situations					1003:1012	various environmental situations	981:1012	various environmental situations (pH, inorganic salts, humic acid)	981:1046	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	2	14	theme	new	399:401	arg1	biochar					403:409	a new biochar adsorbent	397:419	a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal	397:456	Here, an innovative technique combining Fe-Mg-layered double oxides and ball milling was presented for the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal.					
35710050	2	14	theme	new	399:401	arg1	BM-LDOs-BC					422:431	BM-LDOs-BC	422:431	BM-LDOs-BC	422:431	Here, an innovative technique combining Fe-Mg-layered double oxides and ball milling was presented for the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal.					
35710050	0	15	theme	ciprofloxacin	101:113	arg1	removal					126:132	ciprofloxacin adsorptive removal	101:132	ciprofloxacin adsorptive removal from water	101:143	Insights on ball milling enhanced iron magnesium layered double oxides bagasse biochar composite for ciprofloxacin adsorptive removal from water.					
35710050	5	16	dep	situations	1003:1012	arg1	salts					1029:1033	inorganic salts	1019:1033	inorganic salts	1019:1033	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	5	16	dep	situations	1003:1012	arg1	acid					1042:1045	humic acid	1036:1045	humic acid	1036:1045	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	5	16	dep	situations	1003:1012	arg1	pH					1015:1016	pH	1015:1016	pH	1015:1016	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	0	17	from	water	139:143	arg1	removal					126:132	ciprofloxacin adsorptive removal	101:132	ciprofloxacin adsorptive removal from water	101:143	Insights on ball milling enhanced iron magnesium layered double oxides bagasse biochar composite for ciprofloxacin adsorptive removal from water.					
35710050	7	18	theme	antibiotic	1282:1291	arg1	removal					1293:1299	antibiotic removal	1282:1299	antibiotic removal	1282:1299	These superiorities illustrated that BM-LDOs-BC was a promising adsorbent and created a new avenue for rational placement of biowaste and high-efficiency synthesis of biochar for antibiotic removal.					
35710050	2	19	theme	CIP	446:448	arg1	removal					450:456	aqueous CIP removal	438:456	aqueous CIP removal	438:456	Here, an innovative technique combining Fe-Mg-layered double oxides and ball milling was presented for the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal.					
35710050	7	20	theme	biochar	1270:1276	arg1	synthesis					1257:1265	biowaste and high-efficiency synthesis	1228:1265	biowaste and high-efficiency synthesis of biochar	1228:1276	These superiorities illustrated that BM-LDOs-BC was a promising adsorbent and created a new avenue for rational placement of biowaste and high-efficiency synthesis of biochar for antibiotic removal.					
35710050	1	21	theme	sugarcane	175:183	arg1	bagasse					185:191	sugarcane bagasse	175:191	sugarcane bagasse	175:191	Both ciprofloxacin (CIP) and sugarcane bagasse have brought enormous pressure on environmental safety.					
35710050	2	22	theme	aqueous	438:444	arg1	removal					450:456	aqueous CIP removal	438:456	aqueous CIP removal	438:456	Here, an innovative technique combining Fe-Mg-layered double oxides and ball milling was presented for the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal.					
35710050	5	23	theme	environmental	918:930	arg1	safety					932:937	satisfactory environmental safety	905:937	satisfactory environmental safety	905:937	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	2	24	theme	ball	321:324	arg1	milling					326:332	ball milling	321:332	ball milling	321:332	Here, an innovative technique combining Fe-Mg-layered double oxides and ball milling was presented for the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal.					
35710050	5	25	theme	outstanding	943:953	arg1	capacity					966:973	outstanding adsorption capacity	943:973	outstanding adsorption capacity	943:973	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	7	26	theme	synthesis	1257:1265	arg1	placement					1215:1223	rational placement	1206:1223	rational placement of biowaste and high-efficiency synthesis of biochar	1206:1276	These superiorities illustrated that BM-LDOs-BC was a promising adsorbent and created a new avenue for rational placement of biowaste and high-efficiency synthesis of biochar for antibiotic removal.					
35710050	3	27	theme	adsorption	560:569	arg1	sites					571:575	abundant adsorption sites	551:575	abundant adsorption sites provided by well-developed pores	551:608	The maximum theoretical adsorption capacity of BM-LDOs-BC reached up to 213.1 mg g-1 due to abundant adsorption sites provided by well-developed pores characteristics and enhanced functional groups.					
35710050	7	28	theme	new	1191:1193	arg1	avenue					1195:1200	a new avenue	1189:1200	a new avenue for rational placement of biowaste and high-efficiency synthesis of biochar for antibiotic removal	1189:1299	These superiorities illustrated that BM-LDOs-BC was a promising adsorbent and created a new avenue for rational placement of biowaste and high-efficiency synthesis of biochar for antibiotic removal.					
35710050	5	29	theme	humic	1036:1040	arg1	acid					1042:1045	humic acid	1036:1045	humic acid	1036:1045	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	5	29	theme	humic	1036:1040	arg1	pH					1015:1016	pH	1015:1016	pH	1015:1016	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	5	30	theme	inorganic	1019:1027	arg1	salts					1029:1033	inorganic salts	1019:1033	inorganic salts	1019:1033	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	5	30	theme	inorganic	1019:1027	arg1	pH					1015:1016	pH	1015:1016	pH	1015:1016	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	5	31	theme	various	981:987	arg1	situations					1003:1012	various environmental situations	981:1012	various environmental situations (pH, inorganic salts, humic acid)	981:1046	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	3	32	theme	maximum	463:469	arg1	capacity					494:501	The maximum theoretical adsorption capacity	459:501	The maximum theoretical adsorption capacity of BM-LDOs-BC	459:515	The maximum theoretical adsorption capacity of BM-LDOs-BC reached up to 213.1 mg g-1 due to abundant adsorption sites provided by well-developed pores characteristics and enhanced functional groups.					
35710050	0	33	theme	iron	34:37	arg1	oxides					64:69	enhanced iron magnesium layered double oxides	25:69	enhanced iron magnesium layered double oxides	25:69	Insights on ball milling enhanced iron magnesium layered double oxides bagasse biochar composite for ciprofloxacin adsorptive removal from water.					
35710050	1	34	theme	enormous	206:213	arg1	pressure					215:222	enormous pressure	206:222	enormous pressure	206:222	Both ciprofloxacin (CIP) and sugarcane bagasse have brought enormous pressure on environmental safety.					
35710050	4	35	theme	CIP	860:862	arg1	removal					875:881	CIP adsorptive removal	860:881	CIP adsorptive removal	860:881	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	2	36	theme	adsorbent	411:419	arg1	biochar					403:409	a new biochar adsorbent	397:419	a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal	397:456	Here, an innovative technique combining Fe-Mg-layered double oxides and ball milling was presented for the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal.					
35710050	2	36	theme	adsorbent	411:419	arg1	BM-LDOs-BC					422:431	BM-LDOs-BC	422:431	BM-LDOs-BC	422:431	Here, an innovative technique combining Fe-Mg-layered double oxides and ball milling was presented for the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal.					
35710050	0	37	theme	enhanced	25:32	arg1	oxides					64:69	enhanced iron magnesium layered double oxides	25:69	enhanced iron magnesium layered double oxides	25:69	Insights on ball milling enhanced iron magnesium layered double oxides bagasse biochar composite for ciprofloxacin adsorptive removal from water.					
35710050	7	38	theme	high-efficiency	1241:1255	arg1	synthesis					1257:1265	biowaste and high-efficiency synthesis	1228:1265	biowaste and high-efficiency synthesis of biochar	1228:1276	These superiorities illustrated that BM-LDOs-BC was a promising adsorbent and created a new avenue for rational placement of biowaste and high-efficiency synthesis of biochar for antibiotic removal.					
35710050	5	39	theme	satisfactory	905:916	arg1	safety					932:937	satisfactory environmental safety	905:937	satisfactory environmental safety	905:937	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	0	40	theme	layered	49:55	arg1	oxides					64:69	enhanced iron magnesium layered double oxides	25:69	enhanced iron magnesium layered double oxides	25:69	Insights on ball milling enhanced iron magnesium layered double oxides bagasse biochar composite for ciprofloxacin adsorptive removal from water.					
35710050	5	41	theme	adsorption	955:964	arg1	capacity					966:973	outstanding adsorption capacity	943:973	outstanding adsorption capacity	943:973	BM-LDOs-BC exhibited satisfactory environmental safety and outstanding adsorption capacity under various environmental situations (pH, inorganic salts, humic acid).					
35710050	3	42	theme	well-developed	589:602	arg1	pores					604:608	well-developed pores	589:608	well-developed pores	589:608	The maximum theoretical adsorption capacity of BM-LDOs-BC reached up to 213.1 mg g-1 due to abundant adsorption sites provided by well-developed pores characteristics and enhanced functional groups.					
35710050	3	43	dep	213.1 mg g-1	531:542	arg1	to					528:529	to	528:529	to	528:529	The maximum theoretical adsorption capacity of BM-LDOs-BC reached up to 213.1 mg g-1 due to abundant adsorption sites provided by well-developed pores characteristics and enhanced functional groups.					
35710050	0	44	theme	magnesium	39:47	arg1	oxides					64:69	enhanced iron magnesium layered double oxides	25:69	enhanced iron magnesium layered double oxides	25:69	Insights on ball milling enhanced iron magnesium layered double oxides bagasse biochar composite for ciprofloxacin adsorptive removal from water.					
35710050	4	45	theme	adsorptive	864:873	arg1	removal					875:881	CIP adsorptive removal	860:881	CIP adsorptive removal	860:881	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	46	theme	pore	746:749	arg1	filling					751:757	pore filling	746:757	pore filling	746:757	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	46	theme	pore	746:749	arg1	mechanisms					845:854	the key mechanisms	837:854	the key mechanisms for CIP adsorptive removal	837:881	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	46	theme	pore	746:749	arg1	complexation					799:810	complexation	799:810	complexation	799:810	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	46	theme	pore	746:749	arg1	conjugation					820:830	π-π conjugation	816:830	π-π conjugation	816:830	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	46	theme	pore	746:749	arg1	interactions					774:785	electrostatic interactions	760:785	electrostatic interactions	760:785	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	46	theme	pore	746:749	arg1	H-bonding					788:796	H-bonding	788:796	H-bonding	788:796	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	47	theme	key	841:843	arg1	filling					751:757	pore filling	746:757	pore filling	746:757	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	47	theme	key	841:843	arg1	mechanisms					845:854	the key mechanisms	837:854	the key mechanisms for CIP adsorptive removal	837:881	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	47	theme	key	841:843	arg1	complexation					799:810	complexation	799:810	complexation	799:810	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	47	theme	key	841:843	arg1	conjugation					820:830	π-π conjugation	816:830	π-π conjugation	816:830	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	47	theme	key	841:843	arg1	interactions					774:785	electrostatic interactions	760:785	electrostatic interactions	760:785	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	47	theme	key	841:843	arg1	H-bonding					788:796	H-bonding	788:796	H-bonding	788:796	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	7	48	theme	biowaste	1228:1235	arg1	synthesis					1257:1265	biowaste and high-efficiency synthesis	1228:1265	biowaste and high-efficiency synthesis of biochar	1228:1276	These superiorities illustrated that BM-LDOs-BC was a promising adsorbent and created a new avenue for rational placement of biowaste and high-efficiency synthesis of biochar for antibiotic removal.					
35710050	2	49	theme	innovative	258:267	arg1	technique					269:277	an innovative technique	255:277	an innovative technique combining Fe-Mg-layered double oxides and ball milling	255:332	Here, an innovative technique combining Fe-Mg-layered double oxides and ball milling was presented for the first time to convert bagasse-waste into a new biochar adsorbent (BM-LDOs-BC) for aqueous CIP removal.					
35710050	3	50	theme	theoretical	471:481	arg1	capacity					494:501	The maximum theoretical adsorption capacity	459:501	The maximum theoretical adsorption capacity of BM-LDOs-BC	459:515	The maximum theoretical adsorption capacity of BM-LDOs-BC reached up to 213.1 mg g-1 due to abundant adsorption sites provided by well-developed pores characteristics and enhanced functional groups.					
35710050	3	51	theme	due	544:546	arg1	characteristics					610:624	up to 213.1 mg g-1 due to abundant adsorption sites provided by well-developed pores characteristics	525:624	up to 213.1 mg g-1 due to abundant adsorption sites provided by well-developed pores characteristics	525:624	The maximum theoretical adsorption capacity of BM-LDOs-BC reached up to 213.1 mg g-1 due to abundant adsorption sites provided by well-developed pores characteristics and enhanced functional groups.					
35710050	4	52	theme	electrostatic	760:772	arg1	filling					751:757	pore filling	746:757	pore filling	746:757	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	52	theme	electrostatic	760:772	arg1	mechanisms					845:854	the key mechanisms	837:854	the key mechanisms for CIP adsorptive removal	837:881	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	52	theme	electrostatic	760:772	arg1	complexation					799:810	complexation	799:810	complexation	799:810	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	52	theme	electrostatic	760:772	arg1	conjugation					820:830	π-π conjugation	816:830	π-π conjugation	816:830	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	52	theme	electrostatic	760:772	arg1	interactions					774:785	electrostatic interactions	760:785	electrostatic interactions	760:785	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	4	52	theme	electrostatic	760:772	arg1	H-bonding					788:796	H-bonding	788:796	H-bonding	788:796	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	3	53	theme	adsorption	483:492	arg1	capacity					494:501	The maximum theoretical adsorption capacity	459:501	The maximum theoretical adsorption capacity of BM-LDOs-BC	459:515	The maximum theoretical adsorption capacity of BM-LDOs-BC reached up to 213.1 mg g-1 due to abundant adsorption sites provided by well-developed pores characteristics and enhanced functional groups.					
35710050	1	54	theme	environmental	227:239	arg1	safety					241:246	environmental safety	227:246	environmental safety	227:246	Both ciprofloxacin (CIP) and sugarcane bagasse have brought enormous pressure on environmental safety.					
35710050	4	55	theme	parameter	722:730	arg1	results					662:668	The results	658:668	The results of characterization, data fitting and environmental parameter	658:730	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	0	56	theme	double	57:62	arg1	oxides					64:69	enhanced iron magnesium layered double oxides	25:69	enhanced iron magnesium layered double oxides	25:69	Insights on ball milling enhanced iron magnesium layered double oxides bagasse biochar composite for ciprofloxacin adsorptive removal from water.					
35710050	3	57	theme	functional	639:648	arg1	groups					650:655	functional groups	639:655	functional groups	639:655	The maximum theoretical adsorption capacity of BM-LDOs-BC reached up to 213.1 mg g-1 due to abundant adsorption sites provided by well-developed pores characteristics and enhanced functional groups.					
35710050	4	58	theme	environmental	708:720	arg1	parameter					722:730	environmental parameter	708:730	environmental parameter	708:730	The results of characterization, data fitting and environmental parameter revealed that pore filling, electrostatic interactions, H-bonding, complexation and π-π conjugation were the key mechanisms for CIP adsorptive removal.					
35710050	6	59	theme	excellent	1080:1088	arg1	reusability					1090:1100	excellent reusability	1080:1100	excellent reusability	1080:1100	Moreover, BM-LDOs-BC possessed excellent reusability.					
35533377	2	0	theme	infected	346:353	arg1	wounds					355:360	infected wounds	346:360	infected wounds	346:360	It is well worth trying to study the potential of gallium-mediated hydrogel for treating infected wounds.					
35533377	1	1	theme	"	248:248	arg1	trick					250:254	a "Trojan horse" trick	233:254	a "Trojan horse" trick	233:254	The metal gallium has enormous promise in fighting infections by disrupting bacterial iron metabolism via a "Trojan horse" trick.					
35533377	8	2	theme	favorable	1318:1326	arg1	biocompatibility					1328:1343	its favorable biocompatibility	1314:1343	its favorable biocompatibility	1314:1343	Interestingly, in vitro and in vivo results also demonstrated its favorable biocompatibility, reduced bacterial growth, and accelerated infected wound healing, making the gallium-incorporated hydrogel an ideal antimicrobial dressing.					
35533377	8	3	dep	in	1280:1281	arg1	vivo					1283:1286	vivo	1283:1286	vivo	1283:1286	Interestingly, in vitro and in vivo results also demonstrated its favorable biocompatibility, reduced bacterial growth, and accelerated infected wound healing, making the gallium-incorporated hydrogel an ideal antimicrobial dressing.					
35533377	1	4	from	promise	158:164	arg1	infections					178:187	fighting infections	169:187	fighting infections	169:187	The metal gallium has enormous promise in fighting infections by disrupting bacterial iron metabolism via a "Trojan horse" trick.					
35533377	0	5	theme	Infected	103:110	arg1	Healing					118:124	Infected Wound Healing	103:124	Infected Wound Healing	103:124	Gallium(III)-Mediated Dual-Cross-Linked Alginate Hydrogels with Antibacterial Properties for Promoting Infected Wound Healing.					
35533377	6	6	theme	antimicrobial	891:903	arg1	activity					905:912	broad-spectrum antimicrobial activity	876:912	broad-spectrum antimicrobial activity	876:912	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	8	7	dep	in	1267:1268	arg1	vitro					1270:1274	vitro	1270:1274	vitro	1270:1274	Interestingly, in vitro and in vivo results also demonstrated its favorable biocompatibility, reduced bacterial growth, and accelerated infected wound healing, making the gallium-incorporated hydrogel an ideal antimicrobial dressing.					
35533377	6	8	theme	broad-spectrum	876:889	arg1	activity					905:912	broad-spectrum antimicrobial activity	876:912	broad-spectrum antimicrobial activity	876:912	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	6	9	theme	wounds	985:990	arg1	treatment					963:971	the treatment	959:971	the treatment of infected wounds	959:990	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	7	10	theme	mechanical	1164:1173	arg1	performance					1175:1185	the strengthened mechanical performance	1147:1185	the strengthened mechanical performance	1147:1185	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	4	11	theme	infected	684:691	arg1	wounds					693:698	infected wounds	684:698	infected wounds	684:698	It acts nor only as a cross-linking agent to form a hydrogel material but also as a therapeutic agent to slow-release and continuously treat infected wounds.					
35533377	6	12	link	photo-dual-cross-linked	828:850	arg1	hydrogel					861:868	a new gallium ionic- and photo-dual-cross-linked alginate hydrogel	803:868	a new gallium ionic- and photo-dual-cross-linked alginate hydrogel	803:868	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	5	13	theme	Further	701:707	arg1	photo-cross-linking					709:727	Further photo-cross-linking	701:727	Further photo-cross-linking	701:727	Further photo-cross-linking is introduced to improve the mechanical properties of the hydrogel.					
35533377	3	14	theme	metal	453:457	arg1	ions					459:462	metal ions	453:462	metal ions	453:462	Herein, on the basis of a conventional gelation strategy of sodium alginate combined with metal ions, Ga3+ has been innovatively given a dual role in a dual-cross-linked hydrogel.					
35533377	3	15	theme	dual	500:503	arg1	role					505:508	a dual role	498:508	a dual role	498:508	Herein, on the basis of a conventional gelation strategy of sodium alginate combined with metal ions, Ga3+ has been innovatively given a dual role in a dual-cross-linked hydrogel.					
35533377	8	16	theme	in	1267:1268	arg1	results					1288:1294	in vitro and in vivo results	1267:1294	in vitro and in vivo results	1267:1294	Interestingly, in vitro and in vivo results also demonstrated its favorable biocompatibility, reduced bacterial growth, and accelerated infected wound healing, making the gallium-incorporated hydrogel an ideal antimicrobial dressing.					
35533377	1	17	theme	fighting	169:176	arg1	infections					178:187	fighting infections	169:187	fighting infections	169:187	The metal gallium has enormous promise in fighting infections by disrupting bacterial iron metabolism via a "Trojan horse" trick.					
35533377	0	18	theme	Wound	112:116	arg1	Healing					118:124	Infected Wound Healing	103:124	Infected Wound Healing	103:124	Gallium(III)-Mediated Dual-Cross-Linked Alginate Hydrogels with Antibacterial Properties for Promoting Infected Wound Healing.					
35533377	6	19	with	hydrogel	861:868	arg1	activity					905:912	broad-spectrum antimicrobial activity	876:912	broad-spectrum antimicrobial activity	876:912	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	6	19	with	hydrogel	861:868	arg1	performance					942:952	strengthened mechanical performance	918:952	strengthened mechanical performance	918:952	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	3	20	theme	sodium	423:428	arg1	alginate					430:437	sodium alginate	423:437	sodium alginate combined with metal ions	423:462	Herein, on the basis of a conventional gelation strategy of sodium alginate combined with metal ions, Ga3+ has been innovatively given a dual role in a dual-cross-linked hydrogel.					
35533377	7	21	theme	hydrogel	1195:1202	arg1	homogeneous					1131:1141	homogeneous	1131:1141	homogeneous	1131:1141	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	1	22	contain	has	145:147	arg2	promise					158:164	enormous promise	149:164	enormous promise in fighting infections	149:187	The metal gallium has enormous promise in fighting infections by disrupting bacterial iron metabolism via a "Trojan horse" trick.					
35533377	1	22	contain	has	145:147	arg1	gallium					137:143	The metal gallium	127:143	The metal gallium	127:143	The metal gallium has enormous promise in fighting infections by disrupting bacterial iron metabolism via a "Trojan horse" trick.					
35533377	7	23	theme	gallium	1083:1089	arg1	kinetics					1099:1106	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics	1005:1106	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together	1005:1115	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	0	24	theme	Antibacterial	64:76	arg1	Properties					78:87	Antibacterial Properties	64:87	Antibacterial Properties	64:87	Gallium(III)-Mediated Dual-Cross-Linked Alginate Hydrogels with Antibacterial Properties for Promoting Infected Wound Healing.					
35533377	8	25	theme	infected	1388:1395	arg1	healing					1403:1409	infected wound healing	1388:1409	infected wound healing	1388:1409	Interestingly, in vitro and in vivo results also demonstrated its favorable biocompatibility, reduced bacterial growth, and accelerated infected wound healing, making the gallium-incorporated hydrogel an ideal antimicrobial dressing.					
35533377	3	26	theme	gelation	402:409	arg1	strategy					411:418	a conventional gelation strategy	387:418	a conventional gelation strategy of sodium alginate combined with metal ions	387:462	Herein, on the basis of a conventional gelation strategy of sodium alginate combined with metal ions, Ga3+ has been innovatively given a dual role in a dual-cross-linked hydrogel.					
35533377	5	27	theme	hydrogel	787:794	arg1	properties					769:778	the mechanical properties	754:778	the mechanical properties of the hydrogel	754:794	Further photo-cross-linking is introduced to improve the mechanical properties of the hydrogel.					
35533377	6	28	theme	infected	976:983	arg1	wounds					985:990	infected wounds	976:990	infected wounds	976:990	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	7	29	theme	release	1091:1097	arg1	kinetics					1099:1106	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics	1005:1106	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together	1005:1115	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	3	30	theme	dual-cross-linked	515:531	arg1	hydrogel					533:540	a dual-cross-linked hydrogel	513:540	a dual-cross-linked hydrogel	513:540	Herein, on the basis of a conventional gelation strategy of sodium alginate combined with metal ions, Ga3+ has been innovatively given a dual role in a dual-cross-linked hydrogel.					
35533377	1	31	theme	bacterial	203:211	arg1	metabolism					218:227	bacterial iron metabolism	203:227	bacterial iron metabolism	203:227	The metal gallium has enormous promise in fighting infections by disrupting bacterial iron metabolism via a "Trojan horse" trick.					
35533377	6	32	theme	alginate	852:859	arg1	hydrogel					861:868	a new gallium ionic- and photo-dual-cross-linked alginate hydrogel	803:868	a new gallium ionic- and photo-dual-cross-linked alginate hydrogel	803:868	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	8	33	theme	wound	1397:1401	arg1	healing					1403:1409	infected wound healing	1388:1409	infected wound healing	1388:1409	Interestingly, in vitro and in vivo results also demonstrated its favorable biocompatibility, reduced bacterial growth, and accelerated infected wound healing, making the gallium-incorporated hydrogel an ideal antimicrobial dressing.					
35533377	1	34	theme	iron	213:216	arg1	metabolism					218:227	bacterial iron metabolism	203:227	bacterial iron metabolism	203:227	The metal gallium has enormous promise in fighting infections by disrupting bacterial iron metabolism via a "Trojan horse" trick.					
35533377	0	35	theme	Dual-Cross-Linked	22:38	arg1	Hydrogels					49:57	Dual-Cross-Linked Alginate Hydrogels	22:57	Dual-Cross-Linked Alginate Hydrogels with Antibacterial Properties for Promoting Infected Wound Healing	22:124	Gallium(III)-Mediated Dual-Cross-Linked Alginate Hydrogels with Antibacterial Properties for Promoting Infected Wound Healing.					
35533377	7	36	theme	rheological	1055:1065	arg1	properties					1067:1076	rheological properties	1055:1076	rheological properties	1055:1076	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	6	37	theme	photo-dual-cross-linked	828:850	arg1	hydrogel					861:868	a new gallium ionic- and photo-dual-cross-linked alginate hydrogel	803:868	a new gallium ionic- and photo-dual-cross-linked alginate hydrogel	803:868	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	3	38	theme	alginate	430:437	arg1	strategy					411:418	a conventional gelation strategy	387:418	a conventional gelation strategy of sodium alginate combined with metal ions	387:462	Herein, on the basis of a conventional gelation strategy of sodium alginate combined with metal ions, Ga3+ has been innovatively given a dual role in a dual-cross-linked hydrogel.					
35533377	3	39	theme	conventional	389:400	arg1	strategy					411:418	a conventional gelation strategy	387:418	a conventional gelation strategy of sodium alginate combined with metal ions	387:462	Herein, on the basis of a conventional gelation strategy of sodium alginate combined with metal ions, Ga3+ has been innovatively given a dual role in a dual-cross-linked hydrogel.					
35533377	5	40	theme	mechanical	758:767	arg1	properties					769:778	the mechanical properties	754:778	the mechanical properties of the hydrogel	754:794	Further photo-cross-linking is introduced to improve the mechanical properties of the hydrogel.					
35533377	3	41	dep	strategy	411:418	arg1	the					374:376	the	374:376	the	374:376	Herein, on the basis of a conventional gelation strategy of sodium alginate combined with metal ions, Ga3+ has been innovatively given a dual role in a dual-cross-linked hydrogel.					
35533377	3	41	dep	strategy	411:418	arg1	basis					378:382	basis	378:382	basis	378:382	Herein, on the basis of a conventional gelation strategy of sodium alginate combined with metal ions, Ga3+ has been innovatively given a dual role in a dual-cross-linked hydrogel.					
35533377	4	42	theme	hydrogel	595:602	arg1	material					604:611	a hydrogel material	593:611	a hydrogel material	593:611	It acts nor only as a cross-linking agent to form a hydrogel material but also as a therapeutic agent to slow-release and continuously treat infected wounds.					
35533377	7	43	theme	behavior	1045:1052	arg1	kinetics					1099:1106	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics	1005:1106	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together	1005:1115	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	6	44	theme	ionic-	817:822	arg1	hydrogel					861:868	a new gallium ionic- and photo-dual-cross-linked alginate hydrogel	803:868	a new gallium ionic- and photo-dual-cross-linked alginate hydrogel	803:868	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	8	45	theme	gallium-incorporated	1423:1442	arg1	hydrogel					1444:1451	the gallium-incorporated hydrogel	1419:1451	the gallium-incorporated hydrogel	1419:1451	Interestingly, in vitro and in vivo results also demonstrated its favorable biocompatibility, reduced bacterial growth, and accelerated infected wound healing, making the gallium-incorporated hydrogel an ideal antimicrobial dressing.					
35533377	7	46	theme	degradability	1021:1033	arg1	kinetics					1099:1106	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics	1005:1106	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together	1005:1115	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	7	47	theme	properties	1067:1076	arg1	kinetics					1099:1106	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics	1005:1106	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together	1005:1115	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	0	48	theme	Alginate	40:47	arg1	Hydrogels					49:57	Dual-Cross-Linked Alginate Hydrogels	22:57	Dual-Cross-Linked Alginate Hydrogels with Antibacterial Properties for Promoting Infected Wound Healing	22:124	Gallium(III)-Mediated Dual-Cross-Linked Alginate Hydrogels with Antibacterial Properties for Promoting Infected Wound Healing.					
35533377	7	49	theme	gallium	1238:1244	arg1	ions					1246:1249	gallium ions	1238:1249	gallium ions	1238:1249	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	6	50	theme	gallium	809:815	arg1	hydrogel					861:868	a new gallium ionic- and photo-dual-cross-linked alginate hydrogel	803:868	a new gallium ionic- and photo-dual-cross-linked alginate hydrogel	803:868	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	2	51	theme	hydrogel	324:331	arg1	potential					294:302	the potential	290:302	the potential of gallium-mediated hydrogel for treating infected wounds	290:360	It is well worth trying to study the potential of gallium-mediated hydrogel for treating infected wounds.					
35533377	6	52	theme	mechanical	931:940	arg1	performance					942:952	strengthened mechanical performance	918:952	strengthened mechanical performance	918:952	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	6	53	theme	new	805:807	arg1	hydrogel					861:868	a new gallium ionic- and photo-dual-cross-linked alginate hydrogel	803:868	a new gallium ionic- and photo-dual-cross-linked alginate hydrogel	803:868	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	7	54	theme	strengthened	1151:1162	arg1	performance					1175:1185	the strengthened mechanical performance	1147:1185	the strengthened mechanical performance	1147:1185	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	7	55	theme	morphology	1009:1018	arg1	kinetics					1099:1106	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics	1005:1106	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together	1005:1115	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	1	56	theme	metal	131:135	arg1	gallium					137:143	The metal gallium	127:143	The metal gallium	127:143	The metal gallium has enormous promise in fighting infections by disrupting bacterial iron metabolism via a "Trojan horse" trick.					
35533377	4	57	theme	cross-linking	565:577	arg1	agent					579:583	a cross-linking agent	563:583	a cross-linking agent to form a hydrogel material	563:611	It acts nor only as a cross-linking agent to form a hydrogel material but also as a therapeutic agent to slow-release and continuously treat infected wounds.					
35533377	4	57	theme	cross-linking	565:577	arg1	It					543:544	It	543:544	It	543:544	It acts nor only as a cross-linking agent to form a hydrogel material but also as a therapeutic agent to slow-release and continuously treat infected wounds.					
35533377	7	58	theme	swelling	1036:1043	arg1	behavior					1045:1052	swelling behavior	1036:1052	swelling behavior	1036:1052	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	0	59	with	Hydrogels	49:57	arg1	Properties					78:87	Antibacterial Properties	64:87	Antibacterial Properties	64:87	Gallium(III)-Mediated Dual-Cross-Linked Alginate Hydrogels with Antibacterial Properties for Promoting Infected Wound Healing.					
35533377	4	60	theme	therapeutic	627:637	arg1	agent					639:643	a therapeutic agent	625:643	a therapeutic agent to slow-release and continuously treat infected wounds	625:698	It acts nor only as a cross-linking agent to form a hydrogel material but also as a therapeutic agent to slow-release and continuously treat infected wounds.					
35533377	4	60	theme	therapeutic	627:637	arg1	It					543:544	It	543:544	It	543:544	It acts nor only as a cross-linking agent to form a hydrogel material but also as a therapeutic agent to slow-release and continuously treat infected wounds.					
35533377	8	61	theme	bacterial	1354:1362	arg1	growth					1364:1369	bacterial growth	1354:1369	bacterial growth	1354:1369	Interestingly, in vitro and in vivo results also demonstrated its favorable biocompatibility, reduced bacterial growth, and accelerated infected wound healing, making the gallium-incorporated hydrogel an ideal antimicrobial dressing.					
35533377	2	62	theme	gallium-mediated	307:322	arg1	hydrogel					324:331	gallium-mediated hydrogel	307:331	gallium-mediated hydrogel	307:331	It is well worth trying to study the potential of gallium-mediated hydrogel for treating infected wounds.					
35533377	3	63	link	dual-cross-linked	515:531	arg1	hydrogel					533:540	a dual-cross-linked hydrogel	513:540	a dual-cross-linked hydrogel	513:540	Herein, on the basis of a conventional gelation strategy of sodium alginate combined with metal ions, Ga3+ has been innovatively given a dual role in a dual-cross-linked hydrogel.					
35533377	8	64	theme	in	1280:1281	arg1	results					1288:1294	in vitro and in vivo results	1267:1294	in vitro and in vivo results	1267:1294	Interestingly, in vitro and in vivo results also demonstrated its favorable biocompatibility, reduced bacterial growth, and accelerated infected wound healing, making the gallium-incorporated hydrogel an ideal antimicrobial dressing.					
35533377	8	65	theme	antimicrobial	1462:1474	arg1	dressing					1476:1483	an ideal antimicrobial dressing	1453:1483	an ideal antimicrobial dressing	1453:1483	Interestingly, in vitro and in vivo results also demonstrated its favorable biocompatibility, reduced bacterial growth, and accelerated infected wound healing, making the gallium-incorporated hydrogel an ideal antimicrobial dressing.					
35533377	1	66	theme	Trojan	236:241	arg1	trick					250:254	a "Trojan horse" trick	233:254	a "Trojan horse" trick	233:254	The metal gallium has enormous promise in fighting infections by disrupting bacterial iron metabolism via a "Trojan horse" trick.					
35533377	7	67	theme	ions	1246:1249	arg1	release					1227:1233	the release	1223:1233	the release of gallium ions	1223:1249	The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions.					
35533377	6	68	theme	strengthened	918:929	arg1	performance					942:952	strengthened mechanical performance	918:952	strengthened mechanical performance	918:952	Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported.					
35533377	8	69	theme	ideal	1456:1460	arg1	dressing					1476:1483	an ideal antimicrobial dressing	1453:1483	an ideal antimicrobial dressing	1453:1483	Interestingly, in vitro and in vivo results also demonstrated its favorable biocompatibility, reduced bacterial growth, and accelerated infected wound healing, making the gallium-incorporated hydrogel an ideal antimicrobial dressing.					
35533377	1	70	theme	enormous	149:156	arg1	promise					158:164	enormous promise	149:164	enormous promise in fighting infections	149:187	The metal gallium has enormous promise in fighting infections by disrupting bacterial iron metabolism via a "Trojan horse" trick.					
35533377	1	71	theme	horse	243:247	arg1	trick					250:254	a "Trojan horse" trick	233:254	a "Trojan horse" trick	233:254	The metal gallium has enormous promise in fighting infections by disrupting bacterial iron metabolism via a "Trojan horse" trick.					
35543856	5	0	theme	cross-linkers	873:885	arg1	types					864:868	different types	854:868	different types of cross-linkers	854:885	The scaffolds were prepared by freeze-drying method and cross-linked using different types of cross-linkers.					
35543856	10	1	theme	osteogenic-related	1259:1276	arg1	expression					1283:1292	osteogenic-related gene expression	1259:1292	osteogenic-related gene expression	1259:1292	The MC3T3-E1 proliferation, osteogenic-related gene expression, and matrix mineralization were better pronounced in collagen presence and triggered as collagen type I amount was increased.					
35543856	8	2	theme	prominent	1101:1109	arg1	effects					1111:1117	prominent effects	1101:1117	prominent effects	1101:1117	The cross-linker type and collagen content had prominent effects on mechanical strength.					
35543856	1	3	theme	striking	198:205	arg1	advantages					207:216	striking advantages	198:216	striking advantages	198:216	Bio-composite scaffolds mimicking the natural microenvironment of bone tissue offer striking advantages in material-guided bone regeneration.					
35543856	11	4	theme	%	1534:1534	arg1	nHA					1542:1544	1% (w/v) nHA	1533:1544	1% (w/v) nHA	1533:1544	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	11	5	dep	%	1534:1534	arg1	w/v					1537:1539	w/v	1537:1539	w/v	1537:1539	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	3	6	from	stimulation	573:583	arg1	terms					540:544	terms	540:544	terms of scaffold properties	540:567	Herein, a competitive approach was followed to point out an optimized bio-composite scaffold in terms of scaffold properties and stimulation of osteoblast differentiation.					
35543856	2	7	theme	bone	419:422	arg1	engineering					431:441	bone tissue engineering	419:441	bone tissue engineering	419:441	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	10	8	from	pronounced	1333:1342	arg1	presence					1356:1363	collagen presence	1347:1363	collagen presence	1347:1363	The MC3T3-E1 proliferation, osteogenic-related gene expression, and matrix mineralization were better pronounced in collagen presence and triggered as collagen type I amount was increased.					
35543856	7	9	theme	scaffolds	1008:1016	arg1	types					999:1003	All types	995:1003	All types of scaffolds	995:1016	All types of scaffolds displayed highly porous structures.					
35543856	10	10	theme	matrix	1299:1304	arg1	mineralization					1306:1319	matrix mineralization	1299:1319	matrix mineralization	1299:1319	The MC3T3-E1 proliferation, osteogenic-related gene expression, and matrix mineralization were better pronounced in collagen presence and triggered as collagen type I amount was increased.					
35543856	8	11	theme	cross-linker	1058:1069	arg1	type					1071:1074	The cross-linker type	1054:1074	The cross-linker type	1054:1074	The cross-linker type and collagen content had prominent effects on mechanical strength.					
35543856	6	12	theme	mechanical	921:930	arg1	characterization					932:947	the physicochemical and mechanical characterization	897:947	the physicochemical and mechanical characterization	897:947	Based on the physicochemical and mechanical characterization, the scaffolds were eliminated comparatively.					
35543856	2	13	theme	tissue	424:429	arg1	engineering					431:441	bone tissue engineering	419:441	bone tissue engineering	419:441	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	11	14	theme	1	1533:1533	arg1	%					1534:1534	%	1534:1534	%	1534:1534	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	3	15	theme	scaffold	549:556	arg1	properties					558:567	scaffold properties	549:567	scaffold properties	549:567	Herein, a competitive approach was followed to point out an optimized bio-composite scaffold in terms of scaffold properties and stimulation of osteoblast differentiation.					
35543856	3	16	theme	properties	558:567	arg1	terms					540:544	terms	540:544	terms of scaffold properties	540:567	Herein, a competitive approach was followed to point out an optimized bio-composite scaffold in terms of scaffold properties and stimulation of osteoblast differentiation.					
35543856	11	17	theme	Coll	1516:1519	arg1	amounts					1497:1503	equal amounts	1491:1503	equal amounts of Chi and Coll	1491:1519	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	11	17	theme	Coll	1516:1519	arg1	Coll					1516:1519	Coll	1516:1519	Coll	1516:1519	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	11	17	theme	Coll	1516:1519	arg1	Chi					1508:1510	Chi	1508:1510	Chi	1508:1510	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	2	18	from	success	408:414	arg1	engineering					431:441	bone tissue engineering	419:441	bone tissue engineering	419:441	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	2	19	theme	bio-mimicking	350:362	arg1	cues					364:367	potent bio-mimicking cues	343:367	potent bio-mimicking cues	343:367	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	11	20	contain	containing	1480:1489	arg2	Coll					1516:1519	Coll	1516:1519	Coll	1516:1519	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	11	20	contain	containing	1480:1489	arg2	amounts					1497:1503	equal amounts	1491:1503	equal amounts of Chi and Coll	1491:1519	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	11	20	contain	containing	1480:1489	arg2	Chi					1508:1510	Chi	1508:1510	Chi	1508:1510	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	11	20	contain	containing	1480:1489	arg1	scaffolds					1470:1478	glyoxal cross-linked scaffolds	1449:1478	glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll	1449:1519	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	5	21	theme	different	854:862	arg1	types					864:868	different types	854:868	different types of cross-linkers	854:885	The scaffolds were prepared by freeze-drying method and cross-linked using different types of cross-linkers.					
35543856	9	22	theme	Glyoxal	1143:1149	arg1	structures					1164:1173	Glyoxal cross-linked structures	1143:1173	Glyoxal cross-linked structures	1143:1173	Glyoxal cross-linked structures displayed optimum mechanical and structural properties.					
35543856	6	23	theme	physicochemical	901:915	arg1	characterization					932:947	the physicochemical and mechanical characterization	897:947	the physicochemical and mechanical characterization	897:947	Based on the physicochemical and mechanical characterization, the scaffolds were eliminated comparatively.					
35543856	2	24	theme	potent	343:348	arg1	cues					364:367	potent bio-mimicking cues	343:367	potent bio-mimicking cues	343:367	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	11	25	theme	matrix	1605:1610	arg1	mineralization					1612:1625	matrix mineralization	1605:1625	matrix mineralization	1605:1625	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	1	26	theme	material-guided	221:235	arg1	regeneration					242:253	material-guided bone regeneration	221:253	material-guided bone regeneration	221:253	Bio-composite scaffolds mimicking the natural microenvironment of bone tissue offer striking advantages in material-guided bone regeneration.					
35543856	0	27	theme	Chitosan/Collagen	11:27	arg1	Scaffolds					75:83	Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds	11:83	Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering	11:111	Optimizing Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering.					
35543856	8	28	contain	had	1097:1099	arg2	effects					1111:1117	prominent effects	1101:1117	prominent effects	1101:1117	The cross-linker type and collagen content had prominent effects on mechanical strength.					
35543856	8	28	contain	had	1097:1099	arg1	type					1071:1074	The cross-linker type	1054:1074	The cross-linker type	1054:1074	The cross-linker type and collagen content had prominent effects on mechanical strength.					
35543856	8	28	contain	had	1097:1099	arg1	content					1089:1095	collagen content	1080:1095	collagen content	1080:1095	The cross-linker type and collagen content had prominent effects on mechanical strength.					
35543856	11	29	theme	Chi	1508:1510	arg1	amounts					1497:1503	equal amounts	1491:1503	equal amounts of Chi and Coll	1491:1519	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	11	29	theme	Chi	1508:1510	arg1	Coll					1516:1519	Coll	1516:1519	Coll	1516:1519	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	11	29	theme	Chi	1508:1510	arg1	Chi					1508:1510	Chi	1508:1510	Chi	1508:1510	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	11	30	theme	best	1554:1557	arg1	results					1424:1430	The results	1420:1430	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA	1420:1544	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	11	30	theme	best	1554:1557	arg1	candidates					1559:1568	the best candidates	1550:1568	the best candidates for osteoblast differentiation and matrix mineralization	1550:1625	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	1	31	theme	Bio-composite	114:126	arg1	scaffolds					128:136	Bio-composite scaffolds	114:136	Bio-composite scaffolds mimicking the natural microenvironment of bone tissue	114:190	Bio-composite scaffolds mimicking the natural microenvironment of bone tissue offer striking advantages in material-guided bone regeneration.					
35543856	1	32	theme	bone	237:240	arg1	regeneration					242:253	material-guided bone regeneration	221:253	material-guided bone regeneration	221:253	Bio-composite scaffolds mimicking the natural microenvironment of bone tissue offer striking advantages in material-guided bone regeneration.					
35543856	3	33	theme	competitive	454:464	arg1	approach					466:473	a competitive approach	452:473	a competitive approach	452:473	Herein, a competitive approach was followed to point out an optimized bio-composite scaffold in terms of scaffold properties and stimulation of osteoblast differentiation.					
35543856	10	34	from	presence	1356:1363	arg1	proliferation					1244:1256	The MC3T3-E1 proliferation	1231:1256	The MC3T3-E1 proliferation	1231:1256	The MC3T3-E1 proliferation, osteogenic-related gene expression, and matrix mineralization were better pronounced in collagen presence and triggered as collagen type I amount was increased.					
35543856	10	34	from	presence	1356:1363	arg1	pronounced					1333:1342	pronounced	1333:1342	pronounced	1333:1342	The MC3T3-E1 proliferation, osteogenic-related gene expression, and matrix mineralization were better pronounced in collagen presence and triggered as collagen type I amount was increased.					
35543856	9	35	theme	structural	1208:1217	arg1	properties					1219:1228	optimum mechanical and structural properties	1185:1228	optimum mechanical and structural properties	1185:1228	Glyoxal cross-linked structures displayed optimum mechanical and structural properties.					
35543856	2	36	theme	active	381:386	arg1	combination					260:270	The combination	256:270	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues	256:367	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	2	36	theme	active	381:386	arg1	strategy					388:395	an active strategy	378:395	an active strategy to achieve success in bone tissue engineering	378:441	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	9	37	link	cross-linked	1151:1162	arg1	structures					1164:1173	Glyoxal cross-linked structures	1143:1173	Glyoxal cross-linked structures	1143:1173	Glyoxal cross-linked structures displayed optimum mechanical and structural properties.					
35543856	0	38	theme	I/Nanohydroxyapatite	34:53	arg1	Scaffolds					75:83	Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds	11:83	Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering	11:111	Optimizing Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering.					
35543856	0	39	link	Cross-linked	55:66	arg1	Scaffolds					75:83	Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds	11:83	Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering	11:111	Optimizing Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering.					
35543856	8	40	theme	mechanical	1122:1131	arg1	strength					1133:1140	mechanical strength	1122:1140	mechanical strength	1122:1140	The cross-linker type and collagen content had prominent effects on mechanical strength.					
35543856	0	41	theme	Type	29:32	arg1	Scaffolds					75:83	Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds	11:83	Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering	11:111	Optimizing Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering.					
35543856	4	42	theme	type	661:664	arg1	Chi/Coll/nHA					688:699	Chi/Coll/nHA	688:699	Chi/Coll/nHA	688:699	The scaffolds, composed of chitosan/collagen type I/nanohydroxyapatite (Chi/Coll/nHA) as the most attractive components in bone tissue engineering, were analyzed.					
35543856	4	42	theme	type	661:664	arg1	I/nanohydroxyapatite					666:685	chitosan/collagen type I/nanohydroxyapatite	643:685	chitosan/collagen type I/nanohydroxyapatite (Chi/Coll/nHA)	643:700	The scaffolds, composed of chitosan/collagen type I/nanohydroxyapatite (Chi/Coll/nHA) as the most attractive components in bone tissue engineering, were analyzed.					
35543856	11	43	theme	equal	1491:1495	arg1	amounts					1497:1503	equal amounts	1491:1503	equal amounts of Chi and Coll	1491:1519	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	11	43	theme	equal	1491:1495	arg1	Coll					1516:1519	Coll	1516:1519	Coll	1516:1519	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	11	43	theme	equal	1491:1495	arg1	Chi					1508:1510	Chi	1508:1510	Chi	1508:1510	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	3	44	theme	osteoblast	588:597	arg1	differentiation					599:613	osteoblast differentiation	588:613	osteoblast differentiation	588:613	Herein, a competitive approach was followed to point out an optimized bio-composite scaffold in terms of scaffold properties and stimulation of osteoblast differentiation.					
35543856	0	45	theme	Porous	68:73	arg1	Scaffolds					75:83	Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds	11:83	Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering	11:111	Optimizing Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering.					
35543856	4	46	theme	chitosan/collagen	643:659	arg1	Chi/Coll/nHA					688:699	Chi/Coll/nHA	688:699	Chi/Coll/nHA	688:699	The scaffolds, composed of chitosan/collagen type I/nanohydroxyapatite (Chi/Coll/nHA) as the most attractive components in bone tissue engineering, were analyzed.					
35543856	4	46	theme	chitosan/collagen	643:659	arg1	I/nanohydroxyapatite					666:685	chitosan/collagen type I/nanohydroxyapatite	643:685	chitosan/collagen type I/nanohydroxyapatite (Chi/Coll/nHA)	643:700	The scaffolds, composed of chitosan/collagen type I/nanohydroxyapatite (Chi/Coll/nHA) as the most attractive components in bone tissue engineering, were analyzed.					
35543856	5	47	theme	freeze-drying	810:822	arg1	method					824:829	freeze-drying method	810:829	freeze-drying method	810:829	The scaffolds were prepared by freeze-drying method and cross-linked using different types of cross-linkers.					
35543856	4	48	theme	tissue	744:749	arg1	engineering					751:761	bone tissue engineering	739:761	bone tissue engineering	739:761	The scaffolds, composed of chitosan/collagen type I/nanohydroxyapatite (Chi/Coll/nHA) as the most attractive components in bone tissue engineering, were analyzed.					
35543856	4	49	from	components	725:734	arg1	engineering					751:761	bone tissue engineering	739:761	bone tissue engineering	739:761	The scaffolds, composed of chitosan/collagen type I/nanohydroxyapatite (Chi/Coll/nHA) as the most attractive components in bone tissue engineering, were analyzed.					
35543856	3	50	theme	bio-composite	514:526	arg1	scaffold					528:535	an optimized bio-composite scaffold	501:535	an optimized bio-composite scaffold in terms of scaffold properties	501:567	Herein, a competitive approach was followed to point out an optimized bio-composite scaffold in terms of scaffold properties and stimulation of osteoblast differentiation.					
35543856	0	51	theme	Cross-linked	55:66	arg1	Scaffolds					75:83	Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds	11:83	Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering	11:111	Optimizing Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering.					
35543856	9	52	theme	cross-linked	1151:1162	arg1	structures					1164:1173	Glyoxal cross-linked structures	1143:1173	Glyoxal cross-linked structures	1143:1173	Glyoxal cross-linked structures displayed optimum mechanical and structural properties.					
35543856	2	53	theme	polymers	297:304	arg1	combination					260:270	The combination	256:270	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues	256:367	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	2	53	theme	polymers	297:304	arg1	strategy					388:395	an active strategy	378:395	an active strategy to achieve success in bone tissue engineering	378:441	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	4	54	theme	bone	739:742	arg1	engineering					751:761	bone tissue engineering	739:761	bone tissue engineering	739:761	The scaffolds, composed of chitosan/collagen type I/nanohydroxyapatite (Chi/Coll/nHA) as the most attractive components in bone tissue engineering, were analyzed.					
35543856	11	55	theme	cross-linked	1457:1468	arg1	scaffolds					1470:1478	glyoxal cross-linked scaffolds	1449:1478	glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll	1449:1519	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	2	56	theme	natural	289:295	arg1	polymers					297:304	biodegradable natural polymers	275:304	biodegradable natural polymers	275:304	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	3	57	from	scaffold	528:535	arg1	terms					540:544	terms	540:544	terms of scaffold properties	540:567	Herein, a competitive approach was followed to point out an optimized bio-composite scaffold in terms of scaffold properties and stimulation of osteoblast differentiation.					
35543856	1	58	theme	natural	152:158	arg1	microenvironment					160:175	the natural microenvironment	148:175	the natural microenvironment of bone tissue	148:190	Bio-composite scaffolds mimicking the natural microenvironment of bone tissue offer striking advantages in material-guided bone regeneration.					
35543856	10	59	theme	collagen	1347:1354	arg1	presence					1356:1363	collagen presence	1347:1363	collagen presence	1347:1363	The MC3T3-E1 proliferation, osteogenic-related gene expression, and matrix mineralization were better pronounced in collagen presence and triggered as collagen type I amount was increased.					
35543856	10	60	theme	MC3T3-E1	1235:1242	arg1	proliferation					1244:1256	The MC3T3-E1 proliferation	1231:1256	The MC3T3-E1 proliferation	1231:1256	The MC3T3-E1 proliferation, osteogenic-related gene expression, and matrix mineralization were better pronounced in collagen presence and triggered as collagen type I amount was increased.					
35543856	10	60	theme	MC3T3-E1	1235:1242	arg1	pronounced					1333:1342	pronounced	1333:1342	pronounced	1333:1342	The MC3T3-E1 proliferation, osteogenic-related gene expression, and matrix mineralization were better pronounced in collagen presence and triggered as collagen type I amount was increased.					
35543856	2	61	theme	biodegradable	275:287	arg1	polymers					297:304	biodegradable natural polymers	275:304	biodegradable natural polymers	275:304	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	8	62	theme	collagen	1080:1087	arg1	content					1089:1095	collagen content	1080:1095	collagen content	1080:1095	The cross-linker type and collagen content had prominent effects on mechanical strength.					
35543856	9	63	theme	optimum	1185:1191	arg1	properties					1219:1228	optimum mechanical and structural properties	1185:1228	optimum mechanical and structural properties	1185:1228	Glyoxal cross-linked structures displayed optimum mechanical and structural properties.					
35543856	11	64	link	cross-linked	1457:1468	arg1	scaffolds					1470:1478	glyoxal cross-linked scaffolds	1449:1478	glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll	1449:1519	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	9	65	theme	mechanical	1193:1202	arg1	properties					1219:1228	optimum mechanical and structural properties	1185:1228	optimum mechanical and structural properties	1185:1228	Glyoxal cross-linked structures displayed optimum mechanical and structural properties.					
35543856	10	66	theme	type	1391:1394	arg1	amount					1398:1403	collagen type I amount	1382:1403	collagen type I amount	1382:1403	The MC3T3-E1 proliferation, osteogenic-related gene expression, and matrix mineralization were better pronounced in collagen presence and triggered as collagen type I amount was increased.					
35543856	11	67	theme	glyoxal	1449:1455	arg1	scaffolds					1470:1478	glyoxal cross-linked scaffolds	1449:1478	glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll	1449:1519	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
35543856	1	68	theme	bone	180:183	arg1	tissue					185:190	bone tissue	180:190	bone tissue	180:190	Bio-composite scaffolds mimicking the natural microenvironment of bone tissue offer striking advantages in material-guided bone regeneration.					
35543856	7	69	theme	porous	1035:1040	arg1	structures					1042:1051	highly porous structures	1028:1051	highly porous structures	1028:1051	All types of scaffolds displayed highly porous structures.					
35543856	10	70	theme	collagen	1382:1389	arg1	type					1391:1394	collagen type I	1382:1396	collagen type I amount	1382:1403	The MC3T3-E1 proliferation, osteogenic-related gene expression, and matrix mineralization were better pronounced in collagen presence and triggered as collagen type I amount was increased.					
35543856	0	71	theme	Tissue	94:99	arg1	Engineering					101:111	Bone Tissue Engineering	89:111	Bone Tissue Engineering	89:111	Optimizing Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering.					
35543856	2	72	theme	ceramics	320:327	arg1	combination					260:270	The combination	256:270	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues	256:367	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	2	72	theme	ceramics	320:327	arg1	strategy					388:395	an active strategy	378:395	an active strategy to achieve success in bone tissue engineering	378:441	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	1	73	theme	tissue	185:190	arg1	microenvironment					160:175	the natural microenvironment	148:175	the natural microenvironment of bone tissue	148:190	Bio-composite scaffolds mimicking the natural microenvironment of bone tissue offer striking advantages in material-guided bone regeneration.					
35543856	3	74	theme	optimized	504:512	arg1	scaffold					528:535	an optimized bio-composite scaffold	501:535	an optimized bio-composite scaffold in terms of scaffold properties	501:567	Herein, a competitive approach was followed to point out an optimized bio-composite scaffold in terms of scaffold properties and stimulation of osteoblast differentiation.					
35543856	4	75	theme	attractive	714:723	arg1	components					725:734	the most attractive components	705:734	the most attractive components in bone tissue engineering	705:761	The scaffolds, composed of chitosan/collagen type I/nanohydroxyapatite (Chi/Coll/nHA) as the most attractive components in bone tissue engineering, were analyzed.					
35543856	0	76	theme	Bone	89:92	arg1	Engineering					101:111	Bone Tissue Engineering	89:111	Bone Tissue Engineering	89:111	Optimizing Chitosan/Collagen Type I/Nanohydroxyapatite Cross-linked Porous Scaffolds for Bone Tissue Engineering.					
35543856	10	77	theme	gene	1278:1281	arg1	expression					1283:1292	osteogenic-related gene expression	1259:1292	osteogenic-related gene expression	1259:1292	The MC3T3-E1 proliferation, osteogenic-related gene expression, and matrix mineralization were better pronounced in collagen presence and triggered as collagen type I amount was increased.					
35543856	2	78	theme	bioactive	310:318	arg1	ceramics					320:327	bioactive ceramics	310:327	bioactive ceramics	310:327	The combination of biodegradable natural polymers and bioactive ceramics that leverage potent bio-mimicking cues has been an active strategy to achieve success in bone tissue engineering.					
35543856	3	79	theme	differentiation	599:613	arg1	scaffold					528:535	an optimized bio-composite scaffold	501:535	an optimized bio-composite scaffold in terms of scaffold properties	501:567	Herein, a competitive approach was followed to point out an optimized bio-composite scaffold in terms of scaffold properties and stimulation of osteoblast differentiation.					
35543856	3	79	theme	differentiation	599:613	arg1	stimulation					573:583	stimulation	573:583	stimulation of osteoblast differentiation	573:613	Herein, a competitive approach was followed to point out an optimized bio-composite scaffold in terms of scaffold properties and stimulation of osteoblast differentiation.					
35543856	11	80	theme	osteoblast	1574:1583	arg1	differentiation					1585:1599	osteoblast differentiation	1574:1599	osteoblast differentiation	1574:1599	The results highlighted that glyoxal cross-linked scaffolds containing equal amounts of Chi and Coll by mass and 1% (w/v) nHA are the best candidates for osteoblast differentiation and matrix mineralization.					
36963550	6	0	theme	naked	1240:1244	arg1	eye					1246:1248	naked eye	1240:1248	naked eye	1240:1248	Given that PAI and CQDs could respond to different pH values (5-8), the real-time would pH information was provided by the visible light and fluorescent light dual monitoring system by naked eye.					
36963550	7	1	dep	pH	1366:1367	arg1	levels					1369:1374	levels	1369:1374	levels	1369:1374	Moreover, the visible-fluorescent images could be collected and transformed into RGB signals to quantify the would pH levels, avoiding secondary injuries caused by frequent dressing changes.					
36963550	4	2	theme	near	749:752	arg1	irradiation					775:785	near infrared (NIR) light irradiation	749:785	near infrared (NIR) light irradiation	749:785	The composite hydrogels exhibited the outstanding photothermal conversion efficiency with near infrared (NIR) light irradiation, and the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
36963550	0	3	theme	real-time	108:116	arg1	monitoring					118:127	pH real-time monitoring	105:127	pH real-time monitoring	105:127	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel dressings for pH real-time monitoring and accelerated wound healing.					
36963550	5	4	theme	elevated	913:920	arg1	temperature					922:932	the elevated temperature	909:932	the elevated temperature of the composite hydrogels up to ~45 °C	909:972	Meanwhile, the elevated temperature of the composite hydrogels up to ~45 °C was able to stimulate the migration of epidermal cell to accelerate skin repair.					
36963550	5	4	theme	elevated	913:920	arg1	able					978:981	able	978:981	able	978:981	Meanwhile, the elevated temperature of the composite hydrogels up to ~45 °C was able to stimulate the migration of epidermal cell to accelerate skin repair.					
36963550	7	5	theme	frequent	1415:1422	arg1	changes					1433:1439	frequent dressing changes	1415:1439	frequent dressing changes	1415:1439	Moreover, the visible-fluorescent images could be collected and transformed into RGB signals to quantify the would pH levels, avoiding secondary injuries caused by frequent dressing changes.					
36963550	8	6	from	effect	1500:1505	arg1	wounds					1518:1523	chronic wounds	1510:1523	chronic wounds	1510:1523	PAI/CMC/CQDs was demonstrated the significant therapeutic effect on chronic wounds by eliminating bacterial infections and promoting skin repair under the smart RGB monitoring system.					
36963550	0	7	theme	accelerated	133:143	arg1	healing					151:157	accelerated wound healing	133:157	accelerated wound healing	133:157	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel dressings for pH real-time monitoring and accelerated wound healing.					
36963550	8	8	theme	chronic	1510:1516	arg1	wounds					1518:1523	chronic wounds	1510:1523	chronic wounds	1510:1523	PAI/CMC/CQDs was demonstrated the significant therapeutic effect on chronic wounds by eliminating bacterial infections and promoting skin repair under the smart RGB monitoring system.					
36963550	5	9	theme	skin	1042:1045	arg1	repair					1047:1052	skin repair	1042:1052	skin repair	1042:1052	Meanwhile, the elevated temperature of the composite hydrogels up to ~45 °C was able to stimulate the migration of epidermal cell to accelerate skin repair.					
36963550	3	10	theme	hydrogen	542:549	arg1	bonds					551:555	hydrogen bonds	542:555	hydrogen bonds	542:555	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	3	11	theme	bonds	551:555	arg1	cross-linking					525:537	the cross-linking	521:537	the cross-linking	521:537	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	6	12	dep	light	1186:1190	arg1	system					1230:1235	dual monitoring system	1214:1235	dual monitoring system	1214:1235	Given that PAI and CQDs could respond to different pH values (5-8), the real-time would pH information was provided by the visible light and fluorescent light dual monitoring system by naked eye.					
36963550	6	12	dep	light	1186:1190	arg1	provided					1162:1169	provided	1162:1169	was provided by the visible light and fluorescent light dual monitoring system by naked eye	1158:1248	Given that PAI and CQDs could respond to different pH values (5-8), the real-time would pH information was provided by the visible light and fluorescent light dual monitoring system by naked eye.					
36963550	6	12	dep	light	1186:1190	arg1	the					1174:1176	the	1174:1176	the	1174:1176	Given that PAI and CQDs could respond to different pH values (5-8), the real-time would pH information was provided by the visible light and fluorescent light dual monitoring system by naked eye.					
36963550	2	13	theme	wound	344:348	arg1	healing					350:356	wound healing	344:356	wound healing	344:356	Recently, the hydrogel antibacterial materials have attracted extensive attention for preventing infection in wound healing.					
36963550	8	14	theme	smart	1597:1601	arg1	system					1618:1623	the smart RGB monitoring system	1593:1623	the smart RGB monitoring system	1593:1623	PAI/CMC/CQDs was demonstrated the significant therapeutic effect on chronic wounds by eliminating bacterial infections and promoting skin repair under the smart RGB monitoring system.					
36963550	4	15	theme	NIR	764:766	arg1	irradiation					775:785	near infrared (NIR) light irradiation	749:785	near infrared (NIR) light irradiation	749:785	The composite hydrogels exhibited the outstanding photothermal conversion efficiency with near infrared (NIR) light irradiation, and the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
36963550	6	16	theme	different	1096:1104	arg1	5-8					1117:1119	5-8	1117:1119	5-8	1117:1119	Given that PAI and CQDs could respond to different pH values (5-8), the real-time would pH information was provided by the visible light and fluorescent light dual monitoring system by naked eye.					
36963550	6	16	theme	different	1096:1104	arg1	values					1109:1114	different pH values	1096:1114	different pH values (5-8)	1096:1120	Given that PAI and CQDs could respond to different pH values (5-8), the real-time would pH information was provided by the visible light and fluorescent light dual monitoring system by naked eye.					
36963550	5	17	theme	composite	941:949	arg1	hydrogels					951:959	the composite hydrogels	937:959	the composite hydrogels up to ~45 °C	937:972	Meanwhile, the elevated temperature of the composite hydrogels up to ~45 °C was able to stimulate the migration of epidermal cell to accelerate skin repair.					
36963550	1	18	theme	major	208:212	arg1	challenge					223:231	a major clinical challenge	206:231	a major clinical challenge	206:231	Recurrent infection of chronic wounds remains a major clinical challenge.					
36963550	0	19	theme	wound	145:149	arg1	healing					151:157	accelerated wound healing	133:157	accelerated wound healing	133:157	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel dressings for pH real-time monitoring and accelerated wound healing.					
36963550	3	20	theme	sodium	434:439	arg1	CMC					466:468	CMC	466:468	CMC	466:468	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	3	20	theme	sodium	434:439	arg1	cellulose					455:463	sodium carboxymethyl cellulose	434:463	sodium carboxymethyl cellulose (CMC)	434:469	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	5	21	dep	~45 °C	967:972	arg1	up					961:962	up	961:962	up	961:962	Meanwhile, the elevated temperature of the composite hydrogels up to ~45 °C was able to stimulate the migration of epidermal cell to accelerate skin repair.					
36963550	4	22	theme	antibacterial	801:813	arg1	activity					815:822	the high antibacterial activity	792:822	the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)	792:895	The composite hydrogels exhibited the outstanding photothermal conversion efficiency with near infrared (NIR) light irradiation, and the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
36963550	1	23	theme	clinical	214:221	arg1	challenge					223:231	a major clinical challenge	206:231	a major clinical challenge	206:231	Recurrent infection of chronic wounds remains a major clinical challenge.					
36963550	0	24	theme	photo-inspired	11:24	arg1	hydrogel					82:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel	0:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel	0:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel dressings for pH real-time monitoring and accelerated wound healing.					
36963550	8	25	theme	skin	1575:1578	arg1	repair					1580:1585	skin repair	1575:1585	skin repair	1575:1585	PAI/CMC/CQDs was demonstrated the significant therapeutic effect on chronic wounds by eliminating bacterial infections and promoting skin repair under the smart RGB monitoring system.					
36963550	3	26	theme	hybrid	376:381	arg1	hydrogel					383:390	a hybrid hydrogel	374:390	a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs)	374:503	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	7	27	theme	secondary	1386:1394	arg1	injuries					1396:1403	secondary injuries	1386:1403	secondary injuries caused by frequent dressing changes	1386:1439	Moreover, the visible-fluorescent images could be collected and transformed into RGB signals to quantify the would pH levels, avoiding secondary injuries caused by frequent dressing changes.					
36963550	4	28	theme	photothermal	709:720	arg1	efficiency					733:742	the outstanding photothermal conversion efficiency	693:742	the outstanding photothermal conversion efficiency	693:742	The composite hydrogels exhibited the outstanding photothermal conversion efficiency with near infrared (NIR) light irradiation, and the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
36963550	0	29	theme	Integrated	0:9	arg1	hydrogel					82:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel	0:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel	0:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel dressings for pH real-time monitoring and accelerated wound healing.					
36963550	6	30	theme	visible	1178:1184	arg1	light					1186:1190	visible light	1178:1190	visible light	1178:1190	Given that PAI and CQDs could respond to different pH values (5-8), the real-time would pH information was provided by the visible light and fluorescent light dual monitoring system by naked eye.					
36963550	8	31	theme	significant	1476:1486	arg1	effect					1500:1505	the significant therapeutic effect	1472:1505	the significant therapeutic effect on chronic wounds	1472:1523	PAI/CMC/CQDs was demonstrated the significant therapeutic effect on chronic wounds by eliminating bacterial infections and promoting skin repair under the smart RGB monitoring system.					
36963550	3	32	theme	polyvinyl	567:575	arg1	PAI/CMC/CQDs					644:655	PAI/CMC/CQDs	644:655	PAI/CMC/CQDs	644:655	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	3	32	theme	polyvinyl	567:575	arg1	dots					638:641	polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots	567:641	polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs)	567:656	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	4	33	theme	outstanding	697:707	arg1	efficiency					733:742	the outstanding photothermal conversion efficiency	693:742	the outstanding photothermal conversion efficiency	693:742	The composite hydrogels exhibited the outstanding photothermal conversion efficiency with near infrared (NIR) light irradiation, and the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
36963550	0	34	theme	polyvinyl	40:48	arg1	hydrogel					82:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel	0:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel	0:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel dressings for pH real-time monitoring and accelerated wound healing.					
36963550	4	35	theme	light	769:773	arg1	irradiation					775:785	near infrared (NIR) light irradiation	749:785	near infrared (NIR) light irradiation	749:785	The composite hydrogels exhibited the outstanding photothermal conversion efficiency with near infrared (NIR) light irradiation, and the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
36963550	2	36	theme	antibacterial	257:269	arg1	materials					271:279	the hydrogel antibacterial materials	244:279	the hydrogel antibacterial materials	244:279	Recently, the hydrogel antibacterial materials have attracted extensive attention for preventing infection in wound healing.					
36963550	6	37	theme	monitoring	1219:1228	arg1	system					1230:1235	dual monitoring system	1214:1235	dual monitoring system	1214:1235	Given that PAI and CQDs could respond to different pH values (5-8), the real-time would pH information was provided by the visible light and fluorescent light dual monitoring system by naked eye.					
36963550	3	38	theme	carbamino	476:484	arg1	CQDs					499:502	carbamino quantum dot (CQDs)	476:503	carbamino quantum dot (CQDs)	476:503	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	0	39	theme	antibacterial	26:38	arg1	hydrogel					82:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel	0:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel	0:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel dressings for pH real-time monitoring and accelerated wound healing.					
36963550	3	40	theme	quantum	486:492	arg1	CQDs					499:502	carbamino quantum dot (CQDs)	476:503	carbamino quantum dot (CQDs)	476:503	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	0	41	theme	cellulose	72:80	arg1	hydrogel					82:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel	0:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel	0:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel dressings for pH real-time monitoring and accelerated wound healing.					
36963550	4	42	theme	infrared	754:761	arg1	irradiation					775:785	near infrared (NIR) light irradiation	749:785	near infrared (NIR) light irradiation	749:785	The composite hydrogels exhibited the outstanding photothermal conversion efficiency with near infrared (NIR) light irradiation, and the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
36963550	7	43	theme	visible-fluorescent	1265:1283	arg1	images					1285:1290	the visible-fluorescent images	1261:1290	the visible-fluorescent images	1261:1290	Moreover, the visible-fluorescent images could be collected and transformed into RGB signals to quantify the would pH levels, avoiding secondary injuries caused by frequent dressing changes.					
36963550	3	44	theme	cellulose/carbon	613:628	arg1	PAI/CMC/CQDs					644:655	PAI/CMC/CQDs	644:655	PAI/CMC/CQDs	644:655	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	3	44	theme	cellulose/carbon	613:628	arg1	dots					638:641	polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots	567:641	polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs)	567:656	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	7	45	theme	dressing	1424:1431	arg1	changes					1433:1439	frequent dressing changes	1415:1439	frequent dressing changes	1415:1439	Moreover, the visible-fluorescent images could be collected and transformed into RGB signals to quantify the would pH levels, avoiding secondary injuries caused by frequent dressing changes.					
36963550	0	46	theme	alcohol/carboxymethyl	50:70	arg1	hydrogel					82:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel	0:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel	0:89	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel dressings for pH real-time monitoring and accelerated wound healing.					
36963550	3	47	theme	carboxymethyl	441:453	arg1	CMC					466:468	CMC	466:468	CMC	466:468	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	3	47	theme	carboxymethyl	441:453	arg1	cellulose					455:463	sodium carboxymethyl cellulose	434:463	sodium carboxymethyl cellulose (CMC)	434:469	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	3	48	theme	quantum	630:636	arg1	PAI/CMC/CQDs					644:655	PAI/CMC/CQDs	644:655	PAI/CMC/CQDs	644:655	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	3	48	theme	quantum	630:636	arg1	dots					638:641	polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots	567:641	polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs)	567:656	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	4	49	theme	composite	663:671	arg1	hydrogels					673:681	The composite hydrogels	659:681	The composite hydrogels	659:681	The composite hydrogels exhibited the outstanding photothermal conversion efficiency with near infrared (NIR) light irradiation, and the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
36963550	3	50	theme	polyvinyl	400:408	arg1	PAI					428:430	PAI	428:430	PAI	428:430	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	3	50	theme	polyvinyl	400:408	arg1	alcohol					410:416	polyvinyl alcohol	400:416	polyvinyl alcohol	400:416	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	7	51	theme	RGB	1332:1334	arg1	signals					1336:1342	RGB signals	1332:1342	RGB signals	1332:1342	Moreover, the visible-fluorescent images could be collected and transformed into RGB signals to quantify the would pH levels, avoiding secondary injuries caused by frequent dressing changes.					
36963550	3	52	theme	alcohol‑iodine/sodium	577:597	arg1	PAI/CMC/CQDs					644:655	PAI/CMC/CQDs	644:655	PAI/CMC/CQDs	644:655	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	3	52	theme	alcohol‑iodine/sodium	577:597	arg1	dots					638:641	polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots	567:641	polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs)	567:656	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	8	53	theme	monitoring	1607:1616	arg1	system					1618:1623	the smart RGB monitoring system	1593:1623	the smart RGB monitoring system	1593:1623	PAI/CMC/CQDs was demonstrated the significant therapeutic effect on chronic wounds by eliminating bacterial infections and promoting skin repair under the smart RGB monitoring system.					
36963550	8	54	theme	RGB	1603:1605	arg1	system					1618:1623	the smart RGB monitoring system	1593:1623	the smart RGB monitoring system	1593:1623	PAI/CMC/CQDs was demonstrated the significant therapeutic effect on chronic wounds by eliminating bacterial infections and promoting skin repair under the smart RGB monitoring system.					
36963550	2	55	theme	hydrogel	248:255	arg1	materials					271:279	the hydrogel antibacterial materials	244:279	the hydrogel antibacterial materials	244:279	Recently, the hydrogel antibacterial materials have attracted extensive attention for preventing infection in wound healing.					
36963550	3	56	theme	carboxymethyl	599:611	arg1	PAI/CMC/CQDs					644:655	PAI/CMC/CQDs	644:655	PAI/CMC/CQDs	644:655	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	3	56	theme	carboxymethyl	599:611	arg1	dots					638:641	polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots	567:641	polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs)	567:656	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	1	57	theme	Recurrent	160:168	arg1	infection					170:178	Recurrent infection	160:178	Recurrent infection of chronic wounds	160:196	Recurrent infection of chronic wounds remains a major clinical challenge.					
36963550	6	58	theme	fluorescent	1196:1206	arg1	light					1208:1212	fluorescent light	1196:1212	fluorescent light	1196:1212	Given that PAI and CQDs could respond to different pH values (5-8), the real-time would pH information was provided by the visible light and fluorescent light dual monitoring system by naked eye.					
36963550	5	59	theme	cell	1023:1026	arg1	migration					1000:1008	the migration	996:1008	the migration of epidermal cell	996:1026	Meanwhile, the elevated temperature of the composite hydrogels up to ~45 °C was able to stimulate the migration of epidermal cell to accelerate skin repair.					
36963550	6	60	theme	dual	1214:1217	arg1	system					1230:1235	dual monitoring system	1214:1235	dual monitoring system	1214:1235	Given that PAI and CQDs could respond to different pH values (5-8), the real-time would pH information was provided by the visible light and fluorescent light dual monitoring system by naked eye.					
36963550	3	61	theme	dot	494:496	arg1	CQDs					499:502	carbamino quantum dot (CQDs)	476:503	carbamino quantum dot (CQDs)	476:503	In this study, a hybrid hydrogel made of polyvinyl alcohol - iodine (PAI), sodium carboxymethyl cellulose (CMC), and carbamino quantum dot (CQDs) was prepared by the cross-linking of hydrogen bonds, named as polyvinyl alcohol‑iodine/sodium carboxymethyl cellulose/carbon quantum dots (PAI/CMC/CQDs).					
36963550	2	62	from	infection	331:339	arg1	healing					350:356	wound healing	344:356	wound healing	344:356	Recently, the hydrogel antibacterial materials have attracted extensive attention for preventing infection in wound healing.					
36963550	8	63	theme	therapeutic	1488:1498	arg1	effect					1500:1505	the significant therapeutic effect	1472:1505	the significant therapeutic effect on chronic wounds	1472:1523	PAI/CMC/CQDs was demonstrated the significant therapeutic effect on chronic wounds by eliminating bacterial infections and promoting skin repair under the smart RGB monitoring system.					
36963550	4	64	theme	high	796:799	arg1	activity					815:822	the high antibacterial activity	792:822	the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)	792:895	The composite hydrogels exhibited the outstanding photothermal conversion efficiency with near infrared (NIR) light irradiation, and the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
36963550	4	65	theme	conversion	722:731	arg1	efficiency					733:742	the outstanding photothermal conversion efficiency	693:742	the outstanding photothermal conversion efficiency	693:742	The composite hydrogels exhibited the outstanding photothermal conversion efficiency with near infrared (NIR) light irradiation, and the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
36963550	5	66	theme	hydrogels	951:959	arg1	temperature					922:932	the elevated temperature	909:932	the elevated temperature of the composite hydrogels up to ~45 °C	909:972	Meanwhile, the elevated temperature of the composite hydrogels up to ~45 °C was able to stimulate the migration of epidermal cell to accelerate skin repair.					
36963550	5	66	theme	hydrogels	951:959	arg1	able					978:981	able	978:981	able	978:981	Meanwhile, the elevated temperature of the composite hydrogels up to ~45 °C was able to stimulate the migration of epidermal cell to accelerate skin repair.					
36963550	0	67	theme	pH	105:106	arg1	monitoring					118:127	pH real-time monitoring	105:127	pH real-time monitoring	105:127	Integrated photo-inspired antibacterial polyvinyl alcohol/carboxymethyl cellulose hydrogel dressings for pH real-time monitoring and accelerated wound healing.					
36963550	6	68	theme	pH	1106:1107	arg1	5-8					1117:1119	5-8	1117:1119	5-8	1117:1119	Given that PAI and CQDs could respond to different pH values (5-8), the real-time would pH information was provided by the visible light and fluorescent light dual monitoring system by naked eye.					
36963550	6	68	theme	pH	1106:1107	arg1	values					1109:1114	different pH values	1096:1114	different pH values (5-8)	1096:1120	Given that PAI and CQDs could respond to different pH values (5-8), the real-time would pH information was provided by the visible light and fluorescent light dual monitoring system by naked eye.					
36963550	5	69	theme	epidermal	1013:1021	arg1	cell					1023:1026	epidermal cell	1013:1026	epidermal cell	1013:1026	Meanwhile, the elevated temperature of the composite hydrogels up to ~45 °C was able to stimulate the migration of epidermal cell to accelerate skin repair.					
36963550	8	70	theme	bacterial	1540:1548	arg1	infections					1550:1559	bacterial infections	1540:1559	bacterial infections	1540:1559	PAI/CMC/CQDs was demonstrated the significant therapeutic effect on chronic wounds by eliminating bacterial infections and promoting skin repair under the smart RGB monitoring system.					
36963550	1	71	theme	chronic	183:189	arg1	wounds					191:196	chronic wounds	183:196	chronic wounds	183:196	Recurrent infection of chronic wounds remains a major clinical challenge.					
36963550	4	72	dep	aureus	847:852	arg1	aureus					858:863	S. aureus	855:863	S. aureus	855:863	The composite hydrogels exhibited the outstanding photothermal conversion efficiency with near infrared (NIR) light irradiation, and the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
36963550	4	72	dep	aureus	847:852	arg1	coli					891:894	E. coli	888:894	E. coli	888:894	The composite hydrogels exhibited the outstanding photothermal conversion efficiency with near infrared (NIR) light irradiation, and the high antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
36963550	2	73	theme	extensive	296:304	arg1	attention					306:314	extensive attention	296:314	extensive attention	296:314	Recently, the hydrogel antibacterial materials have attracted extensive attention for preventing infection in wound healing.					
36963550	1	74	theme	wounds	191:196	arg1	infection					170:178	Recurrent infection	160:178	Recurrent infection of chronic wounds	160:196	Recurrent infection of chronic wounds remains a major clinical challenge.					
35123751	4	0	theme	scaffold	714:721	arg1	rate					688:691	degradation rate	676:691	degradation rate	676:691	The addition of halloysite nanotubes enhanced the thermal stability, mechanical characteristics, water uptake, and degradation rate of the nanocomposite scaffold.					
35123751	4	0	theme	scaffold	714:721	arg1	stability					619:627	the thermal stability	607:627	the thermal stability	607:627	The addition of halloysite nanotubes enhanced the thermal stability, mechanical characteristics, water uptake, and degradation rate of the nanocomposite scaffold.					
35123751	4	0	theme	scaffold	714:721	arg1	characteristics					641:655	mechanical characteristics	630:655	mechanical characteristics	630:655	The addition of halloysite nanotubes enhanced the thermal stability, mechanical characteristics, water uptake, and degradation rate of the nanocomposite scaffold.					
35123751	4	0	theme	scaffold	714:721	arg1	uptake					664:669	water uptake	658:669	water uptake	658:669	The addition of halloysite nanotubes enhanced the thermal stability, mechanical characteristics, water uptake, and degradation rate of the nanocomposite scaffold.					
35123751	7	1	theme	10 wt	1058:1062	arg1	halloysite					1081:1090	10 wt% curcumin-loaded halloysite	1058:1090	10 wt% curcumin-loaded halloysite	1058:1090	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	2	2	theme	nanotubes	386:394	arg1	incorporation					358:370	incorporation	358:370	incorporation of halloysite nanotubes into it	358:402	The FTIR and XRD analyses revealed that formation of the network and incorporation of halloysite nanotubes into it were successful.					
35123751	2	2	theme	nanotubes	386:394	arg1	formation					329:337	formation	329:337	formation of the network	329:352	The FTIR and XRD analyses revealed that formation of the network and incorporation of halloysite nanotubes into it were successful.					
35123751	5	3	theme	acceptable	817:826	arg1	attachment					833:842	acceptable cell attachment	817:842	acceptable cell attachment	817:842	The nanocomposite scaffold represented good biomineralization, great cell proliferation, and acceptable cell attachment.					
35123751	1	4	from	nanotubes	177:185	arg1	presence					194:201	the presence	190:201	the presence of calcium cations	190:220	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations were used to fabricate a three-dimensional nanocomposite scaffold.					
35123751	2	5	theme	halloysite	375:384	arg1	nanotubes					386:394	halloysite nanotubes	375:394	halloysite nanotubes	375:394	The FTIR and XRD analyses revealed that formation of the network and incorporation of halloysite nanotubes into it were successful.					
35123751	7	6	theme	tissue	1240:1245	arg1	applications					1259:1270	bone tissue engineering applications	1235:1270	bone tissue engineering applications	1235:1270	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	6	7	theme	cumulative	966:975	arg1	release					977:983	cumulative release	966:983	cumulative release	966:983	Furthermore, the capability of the nanocomposite scaffold for curcumin delivery was approved through cell proliferation, cumulative release, and antibacterial studies.					
35123751	7	8	theme	nanocomposite	1039:1051	arg1	proliferation					1018:1030	Cell proliferation	1013:1030	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite	1013:1090	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	0	9	theme	curcumin	109:116	arg1	delivery					118:125	curcumin delivery	109:125	curcumin delivery	109:125	Development of a novel reinforced scaffold based on chitosan/cellulose nanocrystals/halloysite nanotubes for curcumin delivery.					
35123751	7	10	theme	great	1201:1205	arg1	potential					1207:1215	great potential	1201:1215	great potential for being used in bone tissue engineering applications	1201:1270	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	4	11	theme	thermal	611:617	arg1	stability					619:627	the thermal stability	607:627	the thermal stability	607:627	The addition of halloysite nanotubes enhanced the thermal stability, mechanical characteristics, water uptake, and degradation rate of the nanocomposite scaffold.					
35123751	5	12	theme	great	787:791	arg1	proliferation					798:810	great cell proliferation	787:810	great cell proliferation	787:810	The nanocomposite scaffold represented good biomineralization, great cell proliferation, and acceptable cell attachment.					
35123751	7	13	with	nanocomposite	1039:1051	arg1	halloysite					1081:1090	10 wt% curcumin-loaded halloysite	1058:1090	10 wt% curcumin-loaded halloysite	1058:1090	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	7	14	theme	nanocomposite	1172:1184	arg1	scaffold					1186:1193	the prepared nanocomposite scaffold	1159:1193	the prepared nanocomposite scaffold	1159:1193	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	2	15	theme	XRD	302:304	arg1	analyses					306:313	The FTIR and XRD analyses	289:313	analyses	306:313	The FTIR and XRD analyses revealed that formation of the network and incorporation of halloysite nanotubes into it were successful.					
35123751	7	16	dep	nanotubes	1092:1100	arg1	reached					1102:1108	reached	1102:1108	nanotubes reached around 175% after 72 h. Considering the results	1092:1156	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	3	17	theme	higher	462:467	arg1	amounts					469:475	higher amounts	462:475	higher amounts of halloysite nanotubes	462:499	FESEM images showed that the addition of higher amounts of halloysite nanotubes into the scaffold's matrix leads to more and smaller pores.					
35123751	3	17	theme	higher	462:467	arg1	nanotubes					491:499	halloysite nanotubes	480:499	halloysite nanotubes	480:499	FESEM images showed that the addition of higher amounts of halloysite nanotubes into the scaffold's matrix leads to more and smaller pores.					
35123751	5	18	theme	cell	793:796	arg1	proliferation					798:810	great cell proliferation	787:810	great cell proliferation	787:810	The nanocomposite scaffold represented good biomineralization, great cell proliferation, and acceptable cell attachment.					
35123751	6	19	theme	cell	946:949	arg1	proliferation					951:963	cell proliferation	946:963	cell proliferation	946:963	Furthermore, the capability of the nanocomposite scaffold for curcumin delivery was approved through cell proliferation, cumulative release, and antibacterial studies.					
35123751	5	20	theme	cell	828:831	arg1	attachment					833:842	acceptable cell attachment	817:842	acceptable cell attachment	817:842	The nanocomposite scaffold represented good biomineralization, great cell proliferation, and acceptable cell attachment.					
35123751	7	21	theme	curcumin-loaded	1065:1079	arg1	halloysite					1081:1090	10 wt% curcumin-loaded halloysite	1058:1090	10 wt% curcumin-loaded halloysite	1058:1090	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	3	22	theme	amounts	469:475	arg1	addition					450:457	the addition	446:457	the addition of higher amounts of halloysite nanotubes into the scaffold's matrix	446:526	FESEM images showed that the addition of higher amounts of halloysite nanotubes into the scaffold's matrix leads to more and smaller pores.					
35123751	7	23	theme	Cell	1013:1016	arg1	proliferation					1018:1030	Cell proliferation	1013:1030	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite	1013:1090	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	3	24	theme	FESEM	421:425	arg1	images					427:432	FESEM images	421:432	FESEM images	421:432	FESEM images showed that the addition of higher amounts of halloysite nanotubes into the scaffold's matrix leads to more and smaller pores.					
35123751	7	25	theme	bone	1235:1238	arg1	applications					1259:1270	bone tissue engineering applications	1235:1270	bone tissue engineering applications	1235:1270	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	7	26	theme	prepared	1163:1170	arg1	scaffold					1186:1193	the prepared nanocomposite scaffold	1159:1193	the prepared nanocomposite scaffold	1159:1193	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	1	27	theme	calcium	206:212	arg1	cations					214:220	calcium cations	206:220	calcium cations	206:220	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations were used to fabricate a three-dimensional nanocomposite scaffold.					
35123751	0	28	theme	novel	17:21	arg1	scaffold					34:41	a novel reinforced scaffold	15:41	a novel reinforced scaffold based on chitosan/cellulose	15:69	Development of a novel reinforced scaffold based on chitosan/cellulose nanocrystals/halloysite nanotubes for curcumin delivery.					
35123751	5	29	theme	nanocomposite	728:740	arg1	scaffold					742:749	The nanocomposite scaffold	724:749	The nanocomposite scaffold	724:749	The nanocomposite scaffold represented good biomineralization, great cell proliferation, and acceptable cell attachment.					
35123751	1	30	theme	cations	214:220	arg1	presence					194:201	the presence	190:201	the presence of calcium cations	190:220	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations were used to fabricate a three-dimensional nanocomposite scaffold.					
35123751	7	31	theme	engineering	1247:1257	arg1	applications					1259:1270	bone tissue engineering applications	1235:1270	bone tissue engineering applications	1235:1270	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	4	32	theme	nanotubes	588:596	arg1	addition					565:572	The addition	561:572	The addition of halloysite nanotubes	561:596	The addition of halloysite nanotubes enhanced the thermal stability, mechanical characteristics, water uptake, and degradation rate of the nanocomposite scaffold.					
35123751	3	33	theme	smaller	546:552	arg1	pores					554:558	more and smaller pores	537:558	more and smaller pores	537:558	FESEM images showed that the addition of higher amounts of halloysite nanotubes into the scaffold's matrix leads to more and smaller pores.					
35123751	1	34	theme	Chitosan	128:135	arg1	nanotubes					177:185	Chitosan, cellulose nanocrystals, and halloysite nanotubes	128:185	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations	128:220	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations were used to fabricate a three-dimensional nanocomposite scaffold.					
35123751	4	35	theme	water	658:662	arg1	uptake					664:669	water uptake	658:669	water uptake	658:669	The addition of halloysite nanotubes enhanced the thermal stability, mechanical characteristics, water uptake, and degradation rate of the nanocomposite scaffold.					
35123751	0	36	theme	scaffold	34:41	arg1	Development					0:10	Development	0:10	Development of a novel reinforced scaffold based on chitosan/cellulose	0:69	Development of a novel reinforced scaffold based on chitosan/cellulose nanocrystals/halloysite nanotubes for curcumin delivery.					
35123751	4	37	theme	halloysite	577:586	arg1	nanotubes					588:596	halloysite nanotubes	577:596	halloysite nanotubes	577:596	The addition of halloysite nanotubes enhanced the thermal stability, mechanical characteristics, water uptake, and degradation rate of the nanocomposite scaffold.					
35123751	0	38	theme	reinforced	23:32	arg1	scaffold					34:41	a novel reinforced scaffold	15:41	a novel reinforced scaffold based on chitosan/cellulose	15:69	Development of a novel reinforced scaffold based on chitosan/cellulose nanocrystals/halloysite nanotubes for curcumin delivery.					
35123751	5	39	theme	good	763:766	arg1	biomineralization					768:784	good biomineralization	763:784	good biomineralization	763:784	The nanocomposite scaffold represented good biomineralization, great cell proliferation, and acceptable cell attachment.					
35123751	1	40	used	used	227:230	arg2	nanotubes					177:185	Chitosan, cellulose nanocrystals, and halloysite nanotubes	128:185	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations	128:220	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations were used to fabricate a three-dimensional nanocomposite scaffold.					
35123751	3	41	theme	halloysite	480:489	arg1	nanotubes					491:499	halloysite nanotubes	480:499	halloysite nanotubes	480:499	FESEM images showed that the addition of higher amounts of halloysite nanotubes into the scaffold's matrix leads to more and smaller pores.					
35123751	6	42	theme	curcumin	907:914	arg1	delivery					916:923	curcumin delivery	907:923	curcumin delivery	907:923	Furthermore, the capability of the nanocomposite scaffold for curcumin delivery was approved through cell proliferation, cumulative release, and antibacterial studies.					
35123751	1	43	theme	cellulose	138:146	arg1	nanocrystals					148:159	cellulose nanocrystals	138:159	cellulose nanocrystals	138:159	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations were used to fabricate a three-dimensional nanocomposite scaffold.					
35123751	6	44	theme	antibacterial	990:1002	arg1	studies					1004:1010	antibacterial studies	990:1010	antibacterial studies	990:1010	Furthermore, the capability of the nanocomposite scaffold for curcumin delivery was approved through cell proliferation, cumulative release, and antibacterial studies.					
35123751	6	45	theme	scaffold	894:901	arg1	capability					862:871	the capability	858:871	the capability of the nanocomposite scaffold for curcumin delivery	858:923	Furthermore, the capability of the nanocomposite scaffold for curcumin delivery was approved through cell proliferation, cumulative release, and antibacterial studies.					
35123751	6	45	theme	scaffold	894:901	arg1	approved					929:936	approved	929:936	approved	929:936	Furthermore, the capability of the nanocomposite scaffold for curcumin delivery was approved through cell proliferation, cumulative release, and antibacterial studies.					
35123751	2	46	theme	FTIR	293:296	arg1	analyses					306:313	The FTIR and XRD analyses	289:313	analyses	306:313	The FTIR and XRD analyses revealed that formation of the network and incorporation of halloysite nanotubes into it were successful.					
35123751	4	47	theme	nanocomposite	700:712	arg1	scaffold					714:721	the nanocomposite scaffold	696:721	the nanocomposite scaffold	696:721	The addition of halloysite nanotubes enhanced the thermal stability, mechanical characteristics, water uptake, and degradation rate of the nanocomposite scaffold.					
35123751	1	48	theme	nanocrystals	148:159	arg1	nanotubes					177:185	Chitosan, cellulose nanocrystals, and halloysite nanotubes	128:185	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations	128:220	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations were used to fabricate a three-dimensional nanocomposite scaffold.					
35123751	4	49	theme	mechanical	630:639	arg1	characteristics					641:655	mechanical characteristics	630:655	mechanical characteristics	630:655	The addition of halloysite nanotubes enhanced the thermal stability, mechanical characteristics, water uptake, and degradation rate of the nanocomposite scaffold.					
35123751	6	50	theme	nanocomposite	880:892	arg1	scaffold					894:901	the nanocomposite scaffold	876:901	the nanocomposite scaffold for curcumin delivery	876:923	Furthermore, the capability of the nanocomposite scaffold for curcumin delivery was approved through cell proliferation, cumulative release, and antibacterial studies.					
35123751	7	51	theme	%	1063:1063	arg1	halloysite					1081:1090	10 wt% curcumin-loaded halloysite	1058:1090	10 wt% curcumin-loaded halloysite	1058:1090	Cell proliferation of the nanocomposite with 10 wt% curcumin-loaded halloysite nanotubes reached around 175% after 72 h. Considering the results, the prepared nanocomposite scaffold holds great potential for being used in bone tissue engineering applications.					
35123751	2	52	theme	network	346:352	arg1	incorporation					358:370	incorporation	358:370	incorporation of halloysite nanotubes into it	358:402	The FTIR and XRD analyses revealed that formation of the network and incorporation of halloysite nanotubes into it were successful.					
35123751	2	52	theme	network	346:352	arg1	formation					329:337	formation	329:337	formation of the network	329:352	The FTIR and XRD analyses revealed that formation of the network and incorporation of halloysite nanotubes into it were successful.					
35123751	3	53	theme	nanotubes	491:499	arg1	amounts					469:475	higher amounts	462:475	higher amounts of halloysite nanotubes	462:499	FESEM images showed that the addition of higher amounts of halloysite nanotubes into the scaffold's matrix leads to more and smaller pores.					
35123751	3	53	theme	nanotubes	491:499	arg1	nanotubes					491:499	halloysite nanotubes	480:499	halloysite nanotubes	480:499	FESEM images showed that the addition of higher amounts of halloysite nanotubes into the scaffold's matrix leads to more and smaller pores.					
35123751	3	54	theme	more	537:540	arg1	pores					554:558	more and smaller pores	537:558	more and smaller pores	537:558	FESEM images showed that the addition of higher amounts of halloysite nanotubes into the scaffold's matrix leads to more and smaller pores.					
35123751	4	55	theme	degradation	676:686	arg1	rate					688:691	degradation rate	676:691	degradation rate	676:691	The addition of halloysite nanotubes enhanced the thermal stability, mechanical characteristics, water uptake, and degradation rate of the nanocomposite scaffold.					
35123751	1	56	theme	three-dimensional	247:263	arg1	scaffold					279:286	a three-dimensional nanocomposite scaffold	245:286	a three-dimensional nanocomposite scaffold	245:286	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations were used to fabricate a three-dimensional nanocomposite scaffold.					
35123751	1	57	theme	halloysite	166:175	arg1	nanotubes					177:185	Chitosan, cellulose nanocrystals, and halloysite nanotubes	128:185	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations	128:220	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations were used to fabricate a three-dimensional nanocomposite scaffold.					
35123751	1	58	theme	nanocomposite	265:277	arg1	scaffold					279:286	a three-dimensional nanocomposite scaffold	245:286	a three-dimensional nanocomposite scaffold	245:286	Chitosan, cellulose nanocrystals, and halloysite nanotubes in the presence of calcium cations were used to fabricate a three-dimensional nanocomposite scaffold.					
35816988	9	0	theme	dietary	1106:1112	arg1	compounds					1123:1131	dietary phenolic compounds	1106:1131	dietary phenolic compounds	1106:1131	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	0	1	theme	mung	87:90	arg1	bean					92:95	mung bean	87:95	mung bean sprouts under sucrose treatment	87:127	Widely targeted metabolomics analysis characterizes the phenolic compounds profiles in mung bean sprouts under sucrose treatment.					
35816988	1	2	theme	wholesome	164:172	arg1	substances					174:183	the wholesome substances	160:183	the wholesome substances of mung bean sprouts	160:204	Phenolic compounds are one of the wholesome substances of mung bean sprouts, showing numerous health-promoting functions.					
35816988	6	3	theme	Correlation	637:647	arg1	analysis					649:656	Correlation analysis	637:656	Correlation analysis	637:656	Correlation analysis showed 21 phenolics were positively correlated with antioxidant capacity.					
35816988	7	4	theme	antioxidant	772:782	arg1	capacity					784:791	antioxidant capacity	772:791	antioxidant capacity	772:791	The changes in phenolic composition and antioxidant capacity after sucrose treatment were mainly due to the enrichment of phenolic biosynthesis pathways.					
35816988	8	5	theme	sucrose	1032:1038	arg1	treatment					1040:1048	sucrose treatment	1032:1048	sucrose treatment	1032:1048	Moreover, the gene expression and enzyme activity analysis of key phenolic biosynthetic genes contributed to elucidate the phenolic profile under sucrose treatment.					
35816988	9	6	theme	bean	1068:1071	arg1	sprouts					1073:1079	mung bean sprouts	1063:1079	mung bean sprouts	1063:1079	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	9	6	theme	bean	1068:1071	arg1	sources					1095:1101	promising sources	1085:1101	promising sources of dietary phenolic compounds	1085:1131	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	8	7	theme	gene	900:903	arg1	expression					905:914	gene expression	900:914	gene expression	900:914	Moreover, the gene expression and enzyme activity analysis of key phenolic biosynthetic genes contributed to elucidate the phenolic profile under sucrose treatment.					
35816988	7	8	theme	phenolic	747:754	arg1	composition					756:766	phenolic composition	747:766	phenolic composition	747:766	The changes in phenolic composition and antioxidant capacity after sucrose treatment were mainly due to the enrichment of phenolic biosynthesis pathways.					
35816988	0	9	dep	bean	92:95	arg1	sprouts					97:103	sprouts	97:103	sprouts under sucrose treatment	97:127	Widely targeted metabolomics analysis characterizes the phenolic compounds profiles in mung bean sprouts under sucrose treatment.					
35816988	4	10	theme	antioxidant	485:495	arg1	capacity					497:504	The antioxidant capacity	481:504	The antioxidant capacity	481:504	The antioxidant capacity was significantly improved by sucrose.					
35816988	8	11	theme	genes	974:978	arg1	activity					927:934	enzyme activity	920:934	enzyme activity	920:934	Moreover, the gene expression and enzyme activity analysis of key phenolic biosynthetic genes contributed to elucidate the phenolic profile under sucrose treatment.					
35816988	8	11	theme	genes	974:978	arg1	expression					905:914	gene expression	900:914	gene expression	900:914	Moreover, the gene expression and enzyme activity analysis of key phenolic biosynthetic genes contributed to elucidate the phenolic profile under sucrose treatment.					
35816988	5	12	theme	phenolic	617:624	arg1	compounds					626:634	phenolic compounds	617:634	phenolic compounds	617:634	Based on metabolomics, 251 metabolites were detected, of which 106 were phenolic compounds.					
35816988	2	13	theme	compounds	289:297	arg1	profiles					299:306	phenolic compounds profiles	280:306	phenolic compounds profiles of mung bean sprouts	280:327	Here, effects of sucrose on phenolic compounds profiles of mung bean sprouts were investigated.					
35816988	8	14	theme	enzyme	920:925	arg1	activity					927:934	enzyme activity	920:934	enzyme activity	920:934	Moreover, the gene expression and enzyme activity analysis of key phenolic biosynthetic genes contributed to elucidate the phenolic profile under sucrose treatment.					
35816988	7	15	from	changes	736:742	arg1	composition					756:766	phenolic composition	747:766	phenolic composition	747:766	The changes in phenolic composition and antioxidant capacity after sucrose treatment were mainly due to the enrichment of phenolic biosynthesis pathways.					
35816988	7	15	from	changes	736:742	arg1	capacity					784:791	antioxidant capacity	772:791	antioxidant capacity	772:791	The changes in phenolic composition and antioxidant capacity after sucrose treatment were mainly due to the enrichment of phenolic biosynthesis pathways.					
35816988	2	16	theme	phenolic	280:287	arg1	profiles					299:306	phenolic compounds profiles	280:306	phenolic compounds profiles of mung bean sprouts	280:327	Here, effects of sucrose on phenolic compounds profiles of mung bean sprouts were investigated.					
35816988	9	17	theme	sucrose	1137:1143	arg1	treatment					1145:1153	sucrose treatment	1137:1153	sucrose treatment	1137:1153	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	9	17	theme	sucrose	1137:1143	arg1	process					1165:1171	a good process	1158:1171	a good process to produce phenolic-rich mung bean sprouts	1158:1214	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	1	18	theme	substances	174:183	arg1	substances					174:183	the wholesome substances	160:183	the wholesome substances of mung bean sprouts	160:204	Phenolic compounds are one of the wholesome substances of mung bean sprouts, showing numerous health-promoting functions.					
35816988	1	18	theme	substances	174:183	arg1	one					153:155	one	153:155	one	153:155	Phenolic compounds are one of the wholesome substances of mung bean sprouts, showing numerous health-promoting functions.					
35816988	0	19	theme	sucrose	111:117	arg1	treatment					119:127	sucrose treatment	111:127	sucrose treatment	111:127	Widely targeted metabolomics analysis characterizes the phenolic compounds profiles in mung bean sprouts under sucrose treatment.					
35816988	9	20	theme	phenolic-rich	1184:1196	arg1	bean					1203:1206	phenolic-rich mung bean	1184:1206	phenolic-rich mung bean sprouts	1184:1214	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	3	21	theme	5‰	455:456	arg1	sucrose					458:464	5‰ sucrose	455:464	5‰ sucrose	455:464	Results showed that the content and composition of phenolic compounds were significantly altered by 1‰ and 5‰ sucrose, respectively.					
35816988	0	22	theme	targeted	7:14	arg1	analysis					29:36	Widely targeted metabolomics analysis	0:36	Widely targeted metabolomics analysis	0:36	Widely targeted metabolomics analysis characterizes the phenolic compounds profiles in mung bean sprouts under sucrose treatment.					
35816988	2	23	theme	sprouts	321:327	arg1	profiles					299:306	phenolic compounds profiles	280:306	phenolic compounds profiles of mung bean sprouts	280:327	Here, effects of sucrose on phenolic compounds profiles of mung bean sprouts were investigated.					
35816988	1	24	theme	mung	188:191	arg1	bean					193:196	mung bean	188:196	mung bean sprouts	188:204	Phenolic compounds are one of the wholesome substances of mung bean sprouts, showing numerous health-promoting functions.					
35816988	3	25	theme	phenolic	399:406	arg1	compounds					408:416	phenolic compounds	399:416	phenolic compounds	399:416	Results showed that the content and composition of phenolic compounds were significantly altered by 1‰ and 5‰ sucrose, respectively.					
35816988	2	26	theme	bean	316:319	arg1	sprouts					321:327	mung bean sprouts	311:327	mung bean sprouts	311:327	Here, effects of sucrose on phenolic compounds profiles of mung bean sprouts were investigated.					
35816988	2	27	from	effects	258:264	arg1	profiles					299:306	phenolic compounds profiles	280:306	phenolic compounds profiles of mung bean sprouts	280:327	Here, effects of sucrose on phenolic compounds profiles of mung bean sprouts were investigated.					
35816988	1	28	theme	bean	193:196	arg1	sprouts					198:204	mung bean sprouts	188:204	mung bean sprouts	188:204	Phenolic compounds are one of the wholesome substances of mung bean sprouts, showing numerous health-promoting functions.					
35816988	7	29	theme	phenolic	854:861	arg1	pathways					876:883	phenolic biosynthesis pathways	854:883	phenolic biosynthesis pathways	854:883	The changes in phenolic composition and antioxidant capacity after sucrose treatment were mainly due to the enrichment of phenolic biosynthesis pathways.					
35816988	8	30	theme	biosynthetic	961:972	arg1	genes					974:978	key phenolic biosynthetic genes	948:978	key phenolic biosynthetic genes	948:978	Moreover, the gene expression and enzyme activity analysis of key phenolic biosynthetic genes contributed to elucidate the phenolic profile under sucrose treatment.					
35816988	5	31	theme	which	602:606	arg1	compounds					626:634	phenolic compounds	617:634	phenolic compounds	617:634	Based on metabolomics, 251 metabolites were detected, of which 106 were phenolic compounds.					
35816988	2	32	theme	mung	311:314	arg1	bean					316:319	mung bean	311:319	mung bean sprouts	311:327	Here, effects of sucrose on phenolic compounds profiles of mung bean sprouts were investigated.					
35816988	6	33	theme	antioxidant	710:720	arg1	capacity					722:729	antioxidant capacity	710:729	antioxidant capacity	710:729	Correlation analysis showed 21 phenolics were positively correlated with antioxidant capacity.					
35816988	1	34	theme	sprouts	198:204	arg1	substances					174:183	the wholesome substances	160:183	the wholesome substances of mung bean sprouts	160:204	Phenolic compounds are one of the wholesome substances of mung bean sprouts, showing numerous health-promoting functions.					
35816988	0	35	theme	metabolomics	16:27	arg1	analysis					29:36	Widely targeted metabolomics analysis	0:36	Widely targeted metabolomics analysis	0:36	Widely targeted metabolomics analysis characterizes the phenolic compounds profiles in mung bean sprouts under sucrose treatment.					
35816988	9	36	theme	mung	1198:1201	arg1	bean					1203:1206	phenolic-rich mung bean	1184:1206	phenolic-rich mung bean sprouts	1184:1214	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	8	37	theme	phenolic	952:959	arg1	genes					974:978	key phenolic biosynthetic genes	948:978	key phenolic biosynthetic genes	948:978	Moreover, the gene expression and enzyme activity analysis of key phenolic biosynthetic genes contributed to elucidate the phenolic profile under sucrose treatment.					
35816988	7	38	theme	biosynthesis	863:874	arg1	pathways					876:883	phenolic biosynthesis pathways	854:883	phenolic biosynthesis pathways	854:883	The changes in phenolic composition and antioxidant capacity after sucrose treatment were mainly due to the enrichment of phenolic biosynthesis pathways.					
35816988	8	39	theme	key	948:950	arg1	genes					974:978	key phenolic biosynthetic genes	948:978	key phenolic biosynthetic genes	948:978	Moreover, the gene expression and enzyme activity analysis of key phenolic biosynthetic genes contributed to elucidate the phenolic profile under sucrose treatment.					
35816988	9	40	theme	phenolic	1114:1121	arg1	compounds					1123:1131	dietary phenolic compounds	1106:1131	dietary phenolic compounds	1106:1131	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	9	41	from	process	1165:1171	arg1	summary					1054:1060	summary	1054:1060	summary	1054:1060	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	7	42	theme	pathways	876:883	arg1	enrichment					840:849	the enrichment	836:849	the enrichment of phenolic biosynthesis pathways	836:883	The changes in phenolic composition and antioxidant capacity after sucrose treatment were mainly due to the enrichment of phenolic biosynthesis pathways.					
35816988	9	43	from	sources	1095:1101	arg1	summary					1054:1060	summary	1054:1060	summary	1054:1060	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	8	44	dep	expression	905:914	arg1	the					896:898	the	896:898	the	896:898	Moreover, the gene expression and enzyme activity analysis of key phenolic biosynthetic genes contributed to elucidate the phenolic profile under sucrose treatment.					
35816988	8	44	dep	expression	905:914	arg1	analysis					936:943	analysis	936:943	analysis	936:943	Moreover, the gene expression and enzyme activity analysis of key phenolic biosynthetic genes contributed to elucidate the phenolic profile under sucrose treatment.					
35816988	1	45	theme	numerous	215:222	arg1	functions					241:249	numerous health-promoting functions	215:249	numerous health-promoting functions	215:249	Phenolic compounds are one of the wholesome substances of mung bean sprouts, showing numerous health-promoting functions.					
35816988	0	46	theme	compounds	65:73	arg1	profiles					75:82	the phenolic compounds profiles	52:82	the phenolic compounds profiles in mung bean sprouts under sucrose treatment	52:127	Widely targeted metabolomics analysis characterizes the phenolic compounds profiles in mung bean sprouts under sucrose treatment.					
35816988	0	47	from	profiles	75:82	arg1	bean					92:95	mung bean	87:95	mung bean sprouts under sucrose treatment	87:127	Widely targeted metabolomics analysis characterizes the phenolic compounds profiles in mung bean sprouts under sucrose treatment.					
35816988	5	48	theme	251	568:570	arg1	metabolites					572:582	251 metabolites	568:582	251 metabolites	568:582	Based on metabolomics, 251 metabolites were detected, of which 106 were phenolic compounds.					
35816988	0	49	theme	phenolic	56:63	arg1	compounds					65:73	phenolic compounds	56:73	the phenolic compounds profiles in mung bean sprouts under sucrose treatment	52:127	Widely targeted metabolomics analysis characterizes the phenolic compounds profiles in mung bean sprouts under sucrose treatment.					
35816988	6	50	dep	showed	658:663	arg1	correlated					694:703	correlated	694:703	showed 21 phenolics were positively correlated with antioxidant capacity	658:729	Correlation analysis showed 21 phenolics were positively correlated with antioxidant capacity.					
35816988	1	51	theme	Phenolic	130:137	arg1	compounds					139:147	Phenolic compounds	130:147	Phenolic compounds	130:147	Phenolic compounds are one of the wholesome substances of mung bean sprouts, showing numerous health-promoting functions.					
35816988	2	52	theme	sucrose	269:275	arg1	effects					258:264	effects	258:264	effects of sucrose on phenolic compounds profiles of mung bean sprouts	258:327	Here, effects of sucrose on phenolic compounds profiles of mung bean sprouts were investigated.					
35816988	9	53	theme	bean	1203:1206	arg1	sprouts					1208:1214	phenolic-rich mung bean sprouts	1184:1214	phenolic-rich mung bean sprouts	1184:1214	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	1	54	theme	health-promoting	224:239	arg1	functions					241:249	numerous health-promoting functions	215:249	numerous health-promoting functions	215:249	Phenolic compounds are one of the wholesome substances of mung bean sprouts, showing numerous health-promoting functions.					
35816988	3	55	dep	content	372:378	arg1	the					368:370	the	368:370	the	368:370	Results showed that the content and composition of phenolic compounds were significantly altered by 1‰ and 5‰ sucrose, respectively.					
35816988	8	56	theme	phenolic	1009:1016	arg1	profile					1018:1024	the phenolic profile	1005:1024	the phenolic profile	1005:1024	Moreover, the gene expression and enzyme activity analysis of key phenolic biosynthetic genes contributed to elucidate the phenolic profile under sucrose treatment.					
35816988	9	57	theme	good	1160:1163	arg1	treatment					1145:1153	sucrose treatment	1137:1153	sucrose treatment	1137:1153	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	9	57	theme	good	1160:1163	arg1	process					1165:1171	a good process	1158:1171	a good process to produce phenolic-rich mung bean sprouts	1158:1214	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	9	58	theme	promising	1085:1093	arg1	sprouts					1073:1079	mung bean sprouts	1063:1079	mung bean sprouts	1063:1079	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	9	58	theme	promising	1085:1093	arg1	sources					1095:1101	promising sources	1085:1101	promising sources of dietary phenolic compounds	1085:1131	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	9	59	theme	compounds	1123:1131	arg1	treatment					1145:1153	sucrose treatment	1137:1153	sucrose treatment	1137:1153	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	9	59	theme	compounds	1123:1131	arg1	sprouts					1073:1079	mung bean sprouts	1063:1079	mung bean sprouts	1063:1079	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	9	59	theme	compounds	1123:1131	arg1	sources					1095:1101	promising sources	1085:1101	promising sources of dietary phenolic compounds	1085:1131	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	9	59	theme	compounds	1123:1131	arg1	process					1165:1171	a good process	1158:1171	a good process to produce phenolic-rich mung bean sprouts	1158:1214	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	9	60	theme	mung	1063:1066	arg1	bean					1068:1071	mung bean	1063:1071	mung bean sprouts	1063:1079	In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.					
35816988	3	61	theme	compounds	408:416	arg1	composition					384:394	composition	384:394	composition	384:394	Results showed that the content and composition of phenolic compounds were significantly altered by 1‰ and 5‰ sucrose, respectively.					
35816988	3	61	theme	compounds	408:416	arg1	content					372:378	content	372:378	content	372:378	Results showed that the content and composition of phenolic compounds were significantly altered by 1‰ and 5‰ sucrose, respectively.					
35816988	7	62	theme	sucrose	799:805	arg1	treatment					807:815	sucrose treatment	799:815	sucrose treatment	799:815	The changes in phenolic composition and antioxidant capacity after sucrose treatment were mainly due to the enrichment of phenolic biosynthesis pathways.					
36871687	5	0	from	solubility	1216:1225	arg1	water					1100:1104	water	1100:1104	water	1100:1104	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	0	from	solubility	1216:1225	arg1	acid					1230:1233	acid	1230:1233	acid	1230:1233	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	0	from	solubility	1216:1225	arg1	*					1240:1240	a*	1239:1240	a*	1239:1240	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	4	1	theme	tensile	919:925	arg1	strength					927:934	tensile strength	919:934	tensile strength	919:934	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	3	2	theme	Numerical	578:586	arg1	optimization					588:599	Numerical optimization	578:599	Numerical optimization of method variables	578:619	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	5	3	theme	potato	1025:1030	arg1	starch					1032:1037	the potato starch and gellan gum levels	1021:1059	starch	1032:1037	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	3	4	from	permeability	756:767	arg1	acid					727:730	acid	727:730	acid	727:730	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	8	5	theme	gum	1852:1854	arg1	film					1866:1869	a quince seed gum/potato starch/gellan gum composite film	1813:1869	a quince seed gum/potato starch/gellan gum composite film	1813:1869	The results of this study, thus, showed that there was no statistically significant difference between the predicted and laboratory results (p < 0.05), indicating the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film.					
36871687	6	6	theme	biodegradable	1306:1318	arg1	film					1327:1330	the biodegradable edible film	1302:1330	the biodegradable edible film	1302:1330	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	1	7	theme	quince	208:213	arg1	biopolymers					187:197	three different biopolymers	171:197	three different biopolymers	171:197	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	7	theme	quince	208:213	arg1	gum					220:222	quince seed gum	208:222	quince seed gum	208:222	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	4	8	theme	a	982:982	arg1	*					983:983	a*	982:983	a*	982:983	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	3	9	theme	variables	611:619	arg1	optimization					588:599	Numerical optimization	578:599	Numerical optimization of method variables	578:619	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	2	10	theme	acid	506:509	arg1	properties					394:403	the textural properties	381:403	the textural properties of the films	381:416	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	10	theme	acid	506:509	arg1	solubility					511:520	acid solubility	506:520	acid solubility	506:520	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	3	11	theme	maximum	648:654	arg1	solubility					684:693	the maximum Young's modulus and minimum solubility	644:693	the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design	644:783	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	7	12	theme	electron	1477:1484	arg1	microscopy					1486:1495	scanning electron microscopy	1468:1495	scanning electron microscopy	1468:1495	The results of scanning electron microscopy showed that the film had more uniformity, coherence and smoothness, as compared to other films studied.					
36871687	2	13	theme	vapor	425:429	arg1	permeability					431:442	water vapor permeability	419:442	water vapor permeability	419:442	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	13	theme	vapor	425:429	arg1	properties					394:403	the textural properties	381:403	the textural properties of the films	381:416	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	6	14	theme	starch	1416:1421	arg1	levels					1353:1358	the levels	1349:1358	the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively	1349:1450	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	8	15	theme	study	1621:1625	arg1	results					1605:1611	The results	1601:1611	The results of this study	1601:1625	The results of this study, thus, showed that there was no statistically significant difference between the predicted and laboratory results (p < 0.05), indicating the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film.					
36871687	0	16	theme	resulting	108:116	arg1	film					118:121	the resulting film	104:121	the resulting film by Mixture Design	104:139	Investigation of the mixing ratio of quince seed gum, potato starch and gellan gum on the properties of the resulting film by Mixture Design.					
36871687	3	17	theme	water	744:748	arg1	permeability					756:767	minimum water vapor permeability	736:767	minimum water vapor permeability by mixed design	736:783	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	5	18	from	values	1249:1254	arg1	water					1100:1104	water	1100:1104	water	1100:1104	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	18	from	values	1249:1254	arg1	acid					1230:1233	acid	1230:1233	acid	1230:1233	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	18	from	values	1249:1254	arg1	*					1240:1240	a*	1239:1240	a*	1239:1240	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	6	19	theme	seed	1399:1402	arg1	gum					1404:1406	quince seed gum	1392:1406	quince seed gum	1392:1406	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	4	20	theme	*	990:990	arg1	values					992:997	a* and b* values	982:997	a* and b* values	982:997	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	5	21	theme	water	1107:1111	arg1	permeability					1119:1130	water vapor permeability	1107:1130	water vapor permeability	1107:1130	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	6	22	theme	film	1327:1330	arg1	production					1288:1297	the production	1284:1297	the production of the biodegradable edible film	1284:1330	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	3	23	theme	mixed	772:776	arg1	design					778:783	mixed design	772:783	mixed design	772:783	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	4	24	theme	seed	871:874	arg1	gum					876:878	the quince seed gum	860:878	the quince seed gum	860:878	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	5	25	theme	*	1247:1247	arg1	values					1249:1254	b* values	1246:1254	b* values	1246:1254	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	0	26	theme	potato	54:59	arg1	starch					61:66	potato starch	54:66	potato starch	54:66	Investigation of the mixing ratio of quince seed gum, potato starch and gellan gum on the properties of the resulting film by Mixture Design.					
36871687	3	27	dep	maximum	648:654	arg1	modulus					664:670	Young's modulus	656:670	Young's modulus	656:670	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	6	28	theme	%	1386:1386	arg1	levels					1353:1358	the levels	1349:1358	the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively	1349:1450	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	3	29	theme	minimum	705:711	arg1	solubility					713:722	minimum solubility	705:722	minimum solubility in acid	705:730	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	8	30	theme	significant	1673:1683	arg1	difference					1685:1694	no statistically significant difference	1656:1694	no statistically significant difference between the predicted and laboratory results (p < 0.05)	1656:1750	The results of this study, thus, showed that there was no statistically significant difference between the predicted and laboratory results (p < 0.05), indicating the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film.					
36871687	8	31	theme	composite	1856:1864	arg1	film					1866:1869	a quince seed gum/potato starch/gellan gum composite film	1813:1869	a quince seed gum/potato starch/gellan gum composite film	1813:1869	The results of this study, thus, showed that there was no statistically significant difference between the predicted and laboratory results (p < 0.05), indicating the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film.					
36871687	5	32	theme	a	1239:1239	arg1	*					1240:1240	a*	1239:1240	a*	1239:1240	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	33	theme	L	1147:1147	arg1	value					1150:1154	L* value	1147:1154	L* value	1147:1154	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	1	34	theme	three	276:280	arg1	levels					258:263	levels	258:263	levels of zero to three	258:280	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	6	35	theme	gellan	1427:1432	arg1	gum					1434:1436	gellan gum	1427:1436	gellan gum	1427:1436	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	1	36	theme	seed	215:218	arg1	biopolymers					187:197	three different biopolymers	171:197	three different biopolymers	171:197	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	36	theme	seed	215:218	arg1	gum					220:222	quince seed gum	208:222	quince seed gum	208:222	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	6	37	theme	%	1369:1369	arg1	levels					1353:1358	the levels	1349:1358	the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively	1349:1450	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	8	38	theme	good	1768:1771	arg1	fit					1773:1775	the good fit	1764:1775	the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film	1764:1869	The results of this study, thus, showed that there was no statistically significant difference between the predicted and laboratory results (p < 0.05), indicating the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film.					
36871687	2	39	theme	films	553:557	arg1	microstructure					526:539	microstructure	526:539	microstructure	526:539	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	39	theme	films	553:557	arg1	parameters					494:503	color parameters	488:503	color parameters	488:503	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	39	theme	films	553:557	arg1	water-solubility					445:460	water-solubility	445:460	water-solubility	445:460	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	39	theme	films	553:557	arg1	transparency					463:474	transparency	463:474	transparency	463:474	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	39	theme	films	553:557	arg1	properties					394:403	the textural properties	381:403	the textural properties of the films	381:416	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	39	theme	films	553:557	arg1	solubility					511:520	acid solubility	506:520	acid solubility	506:520	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	39	theme	films	553:557	arg1	permeability					431:442	water vapor permeability	419:442	water vapor permeability	419:442	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	39	theme	films	553:557	arg1	thickness					477:485	thickness	477:485	thickness	477:485	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	8	40	theme	laboratory	1722:1731	arg1	results					1733:1739	laboratory results	1722:1739	laboratory results	1722:1739	The results of this study, thus, showed that there was no statistically significant difference between the predicted and laboratory results (p < 0.05), indicating the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film.					
36871687	0	41	theme	ratio	28:32	arg1	Investigation					0:12	Investigation	0:12	Investigation of the mixing ratio of quince	0:42	Investigation of the mixing ratio of quince seed gum, potato starch and gellan gum on the properties of the resulting film by Mixture Design.					
36871687	4	42	theme	gum	876:878	arg1	increase					848:855	the increase	844:855	the increase of the quince seed gum	844:878	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	8	43	theme	model	1784:1788	arg1	fit					1773:1775	the good fit	1764:1775	the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film	1764:1869	The results of this study, thus, showed that there was no statistically significant difference between the predicted and laboratory results (p < 0.05), indicating the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film.					
36871687	2	44	theme	mixed	362:366	arg1	film					375:378	the mixed edible film	358:378	the mixed edible film	358:378	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	3	45	from	solubility	684:693	arg1	water					698:702	water	698:702	water	698:702	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	3	45	from	solubility	684:693	arg1	solubility					713:722	minimum solubility	705:722	minimum solubility in acid	705:730	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	3	45	from	solubility	684:693	arg1	permeability					756:767	minimum water vapor permeability	736:767	minimum water vapor permeability by mixed design	736:783	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	5	46	theme	tensile	1177:1183	arg1	strength					1185:1192	tensile strength	1177:1192	tensile strength	1177:1192	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	0	47	theme	quince	37:42	arg1	ratio					28:32	the mixing ratio	17:32	the mixing ratio of quince	17:42	Investigation of the mixing ratio of quince seed gum, potato starch and gellan gum on the properties of the resulting film by Mixture Design.					
36871687	1	48	theme	gellan	243:248	arg1	biopolymers					187:197	three different biopolymers	171:197	three different biopolymers	171:197	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	48	theme	gellan	243:248	arg1	gum					250:252	gellan gum	243:252	gellan gum	243:252	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	3	49	from	water	698:702	arg1	acid					727:730	acid	727:730	acid	727:730	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	5	50	theme	gellan	1043:1048	arg1	levels					1054:1059	the potato starch and gellan gum levels	1021:1059	levels	1054:1059	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	51	theme	Young	1160:1164	arg1	modulus					1168:1174	Young's modulus	1160:1174	Young's modulus	1160:1174	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	52	theme	levels	1054:1059	arg1	rise					1013:1016	the rise	1009:1016	the rise of the potato starch and gellan gum levels	1009:1059	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	7	53	contain	had	1518:1520	arg2	coherence					1539:1547	coherence	1539:1547	coherence	1539:1547	The results of scanning electron microscopy showed that the film had more uniformity, coherence and smoothness, as compared to other films studied.					
36871687	7	53	contain	had	1518:1520	arg2	smoothness					1553:1562	smoothness	1553:1562	smoothness	1553:1562	The results of scanning electron microscopy showed that the film had more uniformity, coherence and smoothness, as compared to other films studied.					
36871687	7	53	contain	had	1518:1520	arg1	film					1513:1516	the film	1509:1516	the film	1509:1516	The results of scanning electron microscopy showed that the film had more uniformity, coherence and smoothness, as compared to other films studied.					
36871687	7	53	contain	had	1518:1520	arg2	uniformity					1527:1536	uniformity	1527:1536	uniformity	1527:1536	The results of scanning electron microscopy showed that the film had more uniformity, coherence and smoothness, as compared to other films studied.					
36871687	8	54	theme	seed	1822:1825	arg1	film					1866:1869	a quince seed gum/potato starch/gellan gum composite film	1813:1869	a quince seed gum/potato starch/gellan gum composite film	1813:1869	The results of this study, thus, showed that there was no statistically significant difference between the predicted and laboratory results (p < 0.05), indicating the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film.					
36871687	3	55	theme	method	604:609	arg1	variables					611:619	method variables	604:619	method variables	604:619	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	2	56	theme	color	488:492	arg1	parameters					494:503	color parameters	488:503	color parameters	488:503	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	56	theme	color	488:492	arg1	properties					394:403	the textural properties	381:403	the textural properties of the films	381:416	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	8	57	theme	starch/gellan	1838:1850	arg1	film					1866:1869	a quince seed gum/potato starch/gellan gum composite film	1813:1869	a quince seed gum/potato starch/gellan gum composite film	1813:1869	The results of this study, thus, showed that there was no statistically significant difference between the predicted and laboratory results (p < 0.05), indicating the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film.					
36871687	5	58	theme	starch	1032:1037	arg1	rise					1013:1016	the rise	1009:1016	the rise of the potato starch and gellan gum levels	1009:1059	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	59	from	solubility	1086:1095	arg1	water					1100:1104	water	1100:1104	water	1100:1104	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	59	from	solubility	1086:1095	arg1	acid					1230:1233	acid	1230:1233	acid	1230:1233	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	59	from	solubility	1086:1095	arg1	*					1240:1240	a*	1239:1240	a*	1239:1240	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	4	60	theme	*	983:983	arg1	values					992:997	a* and b* values	982:997	a* and b* values	982:997	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	7	61	theme	microscopy	1486:1495	arg1	results					1457:1463	The results	1453:1463	The results of scanning electron microscopy	1453:1495	The results of scanning electron microscopy showed that the film had more uniformity, coherence and smoothness, as compared to other films studied.					
36871687	5	62	from	thickness	1075:1083	arg1	water					1100:1104	water	1100:1104	water	1100:1104	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	62	from	thickness	1075:1083	arg1	acid					1230:1233	acid	1230:1233	acid	1230:1233	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	62	from	thickness	1075:1083	arg1	*					1240:1240	a*	1239:1240	a*	1239:1240	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	4	63	theme	b	989:989	arg1	*					990:990	b*	989:990	b*	989:990	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	7	64	theme	scanning	1468:1475	arg1	microscopy					1486:1495	scanning electron microscopy	1468:1495	scanning electron microscopy	1468:1495	The results of scanning electron microscopy showed that the film had more uniformity, coherence and smoothness, as compared to other films studied.					
36871687	3	65	theme	Young	656:660	arg1	modulus					664:670	Young's modulus	656:670	Young's modulus	656:670	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	6	66	theme	optimal	1261:1267	arg1	conditions					1269:1278	The optimal conditions	1257:1278	The optimal conditions for the production of the biodegradable edible film	1257:1330	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	2	67	theme	water	419:423	arg1	permeability					431:442	water vapor permeability	419:442	water vapor permeability	419:442	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	67	theme	water	419:423	arg1	properties					394:403	the textural properties	381:403	the textural properties of the films	381:416	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	6	68	theme	potato	1409:1414	arg1	starch					1416:1421	potato starch	1409:1421	potato starch	1409:1421	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	3	69	theme	minimum	676:682	arg1	solubility					684:693	the maximum Young's modulus and minimum solubility	644:693	the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design	644:783	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	0	70	theme	film	118:121	arg1	properties					90:99	the properties	86:99	the properties of the resulting film by Mixture Design	86:139	Investigation of the mixing ratio of quince seed gum, potato starch and gellan gum on the properties of the resulting film by Mixture Design.					
36871687	1	71	theme	biodegradable	301:313	arg1	film					315:318	the biodegradable film	297:318	the biodegradable film	297:318	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	72	from	impact	161:166	arg1	levels					258:263	levels	258:263	levels of zero to three	258:280	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	4	73	from	solubility	958:967	arg1	acid					972:975	acid	972:975	acid	972:975	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	0	74	theme	Mixture	126:132	arg1	Design					134:139	Mixture Design	126:139	Mixture Design	126:139	Investigation of the mixing ratio of quince seed gum, potato starch and gellan gum on the properties of the resulting film by Mixture Design.					
36871687	3	75	theme	vapor	750:754	arg1	permeability					756:767	minimum water vapor permeability	736:767	minimum water vapor permeability by mixed design	736:783	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	6	76	theme	quince	1392:1397	arg1	gum					1404:1406	quince seed gum	1392:1406	quince seed gum	1392:1406	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	8	77	theme	quince	1815:1820	arg1	film					1866:1869	a quince seed gum/potato starch/gellan gum composite film	1813:1869	a quince seed gum/potato starch/gellan gum composite film	1813:1869	The results of this study, thus, showed that there was no statistically significant difference between the predicted and laboratory results (p < 0.05), indicating the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film.					
36871687	4	78	from	elongation	937:946	arg1	acid					972:975	acid	972:975	acid	972:975	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	3	79	theme	minimum	736:742	arg1	permeability					756:767	minimum water vapor permeability	736:767	minimum water vapor permeability by mixed design	736:783	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	2	80	theme	textural	385:392	arg1	microstructure					526:539	microstructure	526:539	microstructure	526:539	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	80	theme	textural	385:392	arg1	parameters					494:503	color parameters	488:503	color parameters	488:503	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	80	theme	textural	385:392	arg1	water-solubility					445:460	water-solubility	445:460	water-solubility	445:460	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	80	theme	textural	385:392	arg1	transparency					463:474	transparency	463:474	transparency	463:474	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	80	theme	textural	385:392	arg1	properties					394:403	the textural properties	381:403	the textural properties of the films	381:416	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	80	theme	textural	385:392	arg1	solubility					511:520	acid solubility	506:520	acid solubility	506:520	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	80	theme	textural	385:392	arg1	permeability					431:442	water vapor permeability	419:442	water vapor permeability	419:442	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	80	theme	textural	385:392	arg1	thickness					477:485	thickness	477:485	thickness	477:485	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	6	81	theme	edible	1320:1325	arg1	film					1327:1330	the biodegradable edible film	1302:1330	the biodegradable edible film	1302:1330	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	4	82	from	strength	927:934	arg1	acid					972:975	acid	972:975	acid	972:975	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	4	83	theme	quince	864:869	arg1	gum					876:878	the quince seed gum	860:878	the quince seed gum	860:878	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	2	84	theme	made	548:551	arg1	films					553:557	the made films	544:557	the made films	544:557	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	0	85	theme	gellan	72:77	arg1	gum					79:81	gellan gum	72:81	gellan gum	72:81	Investigation of the mixing ratio of quince seed gum, potato starch and gellan gum on the properties of the resulting film by Mixture Design.					
36871687	2	86	theme	films	412:416	arg1	microstructure					526:539	microstructure	526:539	microstructure	526:539	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	86	theme	films	412:416	arg1	parameters					494:503	color parameters	488:503	color parameters	488:503	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	86	theme	films	412:416	arg1	water-solubility					445:460	water-solubility	445:460	water-solubility	445:460	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	86	theme	films	412:416	arg1	transparency					463:474	transparency	463:474	transparency	463:474	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	86	theme	films	412:416	arg1	properties					394:403	the textural properties	381:403	the textural properties of the films	381:416	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	86	theme	films	412:416	arg1	solubility					511:520	acid solubility	506:520	acid solubility	506:520	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	86	theme	films	412:416	arg1	permeability					431:442	water vapor permeability	419:442	water vapor permeability	419:442	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	86	theme	films	412:416	arg1	thickness					477:485	thickness	477:485	thickness	477:485	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	2	87	dep	order	341:345	arg1	prepare					350:356	prepare	350:356	to prepare the mixed edible film	347:378	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	6	88	theme	%	1378:1378	arg1	levels					1353:1358	the levels	1349:1358	the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively	1349:1450	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	3	89	theme	Design-Expert	796:808	arg1	software					810:817	the Design-Expert software	792:817	the Design-Expert software	792:817	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	6	90	theme	gum	1434:1436	arg1	levels					1353:1358	the levels	1349:1358	the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively	1349:1450	The optimal conditions for the production of the biodegradable edible film were selected at the levels of 1.623 %, 1.637 % and 0 % for quince seed gum, potato starch and gellan gum, respectively.					
36871687	5	91	theme	*	1148:1148	arg1	value					1150:1154	L* value	1147:1154	L* value	1147:1154	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	4	92	theme	Young	902:906	arg1	modulus					910:916	the Young's modulus	898:916	the Young's modulus	898:916	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	5	93	theme	b	1246:1246	arg1	values					1249:1254	b* values	1246:1254	b* values	1246:1254	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	94	theme	vapor	1113:1117	arg1	permeability					1119:1130	water vapor permeability	1107:1130	water vapor permeability	1107:1130	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	0	95	theme	mixing	21:26	arg1	ratio					28:32	the mixing ratio	17:32	the mixing ratio of quince	17:42	Investigation of the mixing ratio of quince seed gum, potato starch and gellan gum on the properties of the resulting film by Mixture Design.					
36871687	4	96	from	modulus	910:916	arg1	acid					972:975	acid	972:975	acid	972:975	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	5	97	from	strength	1185:1192	arg1	water					1100:1104	water	1100:1104	water	1100:1104	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	97	from	strength	1185:1192	arg1	acid					1230:1233	acid	1230:1233	acid	1230:1233	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	97	from	strength	1185:1192	arg1	*					1240:1240	a*	1239:1240	a*	1239:1240	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	3	98	from	solubility	713:722	arg1	acid					727:730	acid	727:730	acid	727:730	Numerical optimization of method variables was performed based on the maximum Young's modulus and minimum solubility in water, minimum solubility in acid and minimum water vapor permeability by mixed design, using the Design-Expert software.					
36871687	1	99	from	levels	258:263	arg1	gum					250:252	gellan gum	243:252	gellan gum	243:252	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	99	from	levels	258:263	arg1	biopolymers					187:197	three different biopolymers	171:197	three different biopolymers	171:197	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	99	from	levels	258:263	arg1	gum					220:222	quince seed gum	208:222	quince seed gum	208:222	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	99	from	levels	258:263	arg1	starch					232:237	potato starch	225:237	potato starch	225:237	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	99	from	levels	258:263	arg1	impact					161:166	the impact	157:166	the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film	157:318	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	100	theme	potato	225:230	arg1	biopolymers					187:197	three different biopolymers	171:197	three different biopolymers	171:197	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	100	theme	potato	225:230	arg1	starch					232:237	potato starch	225:237	potato starch	225:237	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	2	101	theme	edible	368:373	arg1	film					375:378	the mixed edible film	358:378	the mixed edible film	358:378	In order to prepare the mixed edible film, the textural properties of the films, water vapor permeability, water-solubility, transparency, thickness, color parameters, acid solubility and microstructure of the made films were investigated.					
36871687	1	102	dep	three	276:280	arg1	to					273:274	to	273:274	to	273:274	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	103	theme	different	177:185	arg1	biopolymers					187:197	three different biopolymers	171:197	three different biopolymers	171:197	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	103	theme	different	177:185	arg1	gum					220:222	quince seed gum	208:222	quince seed gum	208:222	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	103	theme	different	177:185	arg1	starch					232:237	potato starch	225:237	potato starch	225:237	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	1	103	theme	different	177:185	arg1	gum					250:252	gellan gum	243:252	gellan gum	243:252	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36871687	7	104	theme	other	1580:1584	arg1	films					1586:1590	other films	1580:1590	other films studied	1580:1598	The results of scanning electron microscopy showed that the film had more uniformity, coherence and smoothness, as compared to other films studied.					
36871687	5	105	from	elongation	1195:1204	arg1	water					1100:1104	water	1100:1104	water	1100:1104	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	105	from	elongation	1195:1204	arg1	acid					1230:1233	acid	1230:1233	acid	1230:1233	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	105	from	elongation	1195:1204	arg1	*					1240:1240	a*	1239:1240	a*	1239:1240	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	5	106	theme	gum	1050:1052	arg1	levels					1054:1059	the potato starch and gellan gum levels	1021:1059	levels	1054:1059	However, the rise of the potato starch and gellan gum levels increased the thickness, solubility in water, water vapor permeability, transparency, L* value and Young's modulus, tensile strength, elongation to break, solubility in acid and a* and b* values.					
36871687	4	107	from	values	992:997	arg1	acid					972:975	acid	972:975	acid	972:975	The results showed that the increase of the quince seed gum directly affected the Young's modulus, tensile strength, elongation to break, solubility in acid, and a* and b* values.					
36871687	8	108	theme	gum/potato	1827:1836	arg1	film					1866:1869	a quince seed gum/potato starch/gellan gum composite film	1813:1869	a quince seed gum/potato starch/gellan gum composite film	1813:1869	The results of this study, thus, showed that there was no statistically significant difference between the predicted and laboratory results (p < 0.05), indicating the good fit of the model designed for producing a quince seed gum/potato starch/gellan gum composite film.					
36871687	1	109	theme	biopolymers	187:197	arg1	impact					161:166	the impact	157:166	the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film	157:318	In this study, the impact of three different biopolymers, namely, quince seed gum, potato starch and gellan gum, at levels of zero to three, on optimizing the biodegradable film was investigated.					
36332282	1	0	theme	nuclear	144:150	arg1	industry					152:159	nuclear industry	144:159	nuclear industry	144:159	The capture of radioactive iodine is an inevitable requirement in nuclear industry for environmental protection.					
36332282	7	1	theme	adsorption	1076:1085	arg1	capacity					1087:1094	The adsorption capacity	1072:1094	The adsorption capacity of UiO-66-NH2 @WCA	1072:1113	The adsorption capacity of UiO-66-NH2 @WCA was highest in solution when the pH was 6, while the ionic strength had little effect.					
36332282	7	1	theme	adsorption	1076:1085	arg1	highest					1119:1125	highest	1119:1125	highest	1119:1125	The adsorption capacity of UiO-66-NH2 @WCA was highest in solution when the pH was 6, while the ionic strength had little effect.					
36332282	5	2	theme	high	840:843	arg1	capacity					856:863	a high adsorption capacity	838:863	a high adsorption capacity of 704 mg/g for iodine vapor	838:892	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	3	3	theme	lower	470:474	arg1	loading					476:482	the lower loading	466:482	the lower loading	466:482	Although the loading of MOFs on wood can avoid the drawbacks of the powder form of MOFs in implementation, the dense structure of wood results in the lower loading, even after delignification, which limits the adsorption capacity.					
36332282	6	4	theme	adsorption	940:949	arg1	behavior					951:958	The adsorption behavior	936:958	The adsorption behavior in iodine aqueous solution	936:985	The adsorption behavior in iodine aqueous solution was well predicted by the Freundlich isotherm and pseudo-second-order kinetic model.					
36332282	6	5	theme	kinetic	1057:1063	arg1	model					1065:1069	pseudo-second-order kinetic model	1037:1069	pseudo-second-order kinetic model	1037:1069	The adsorption behavior in iodine aqueous solution was well predicted by the Freundlich isotherm and pseudo-second-order kinetic model.					
36332282	2	6	theme	iodine	288:293	arg1	adsorption					295:304	iodine adsorption	288:304	iodine adsorption	288:304	Metal-organic frameworks (MOFs) are a new generation of sorbents that have wide applications for iodine adsorption and recovery.					
36332282	9	7	theme	packed	1344:1349	arg1	system					1358:1363	a packed column system	1342:1363	a packed column system	1342:1363	Furthermore, a packed column system was applied to demonstrate the excellent recyclability and potential for practical application.					
36332282	5	8	theme	iodine	881:886	arg1	vapor					888:892	iodine vapor	881:892	iodine vapor	881:892	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	9	9	theme	practical	1438:1446	arg1	application					1448:1458	practical application	1438:1458	practical application	1438:1458	Furthermore, a packed column system was applied to demonstrate the excellent recyclability and potential for practical application.					
36332282	5	10	theme	iodine	911:916	arg1	solution					926:933	iodine aqueous solution	911:933	iodine aqueous solution	911:933	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	1	11	from	requirement	129:139	arg1	industry					152:159	nuclear industry	144:159	nuclear industry	144:159	The capture of radioactive iodine is an inevitable requirement in nuclear industry for environmental protection.					
36332282	4	12	theme	wood-derived	662:673	arg1	aerogel					685:691	wood-derived cellulose aerogel	662:691	wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood	662:758	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	4	12	theme	wood-derived	662:673	arg1	WCA					694:696	WCA	694:696	WCA	694:696	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	4	13	from	synthesis	635:643	arg1	aerogel					685:691	wood-derived cellulose aerogel	662:691	wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood	662:758	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	4	13	from	synthesis	635:643	arg1	WCA					694:696	WCA	694:696	WCA	694:696	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	3	14	theme	wood	450:453	arg1	structure					437:445	the dense structure	427:445	the dense structure of wood	427:453	Although the loading of MOFs on wood can avoid the drawbacks of the powder form of MOFs in implementation, the dense structure of wood results in the lower loading, even after delignification, which limits the adsorption capacity.					
36332282	2	15	theme	wide	266:269	arg1	applications					271:282	wide applications	266:282	wide applications for iodine adsorption and recovery	266:317	Metal-organic frameworks (MOFs) are a new generation of sorbents that have wide applications for iodine adsorption and recovery.					
36332282	6	16	theme	Freundlich	1013:1022	arg1	isotherm					1024:1031	the Freundlich isotherm	1009:1031	the Freundlich isotherm	1009:1031	The adsorption behavior in iodine aqueous solution was well predicted by the Freundlich isotherm and pseudo-second-order kinetic model.					
36332282	8	17	theme	transfer	1253:1260	arg1	effect					1262:1267	a charge transfer effect	1244:1267	a charge transfer effect	1244:1267	The hydroxyl groups on the WCA matrix had a charge transfer effect with iodine, providing additional sites for iodine capture.					
36332282	3	18	theme	MOFs	344:347	arg1	loading					333:339	the loading	329:339	the loading of MOFs on wood	329:355	Although the loading of MOFs on wood can avoid the drawbacks of the powder form of MOFs in implementation, the dense structure of wood results in the lower loading, even after delignification, which limits the adsorption capacity.					
36332282	5	19	theme	adsorption	845:854	arg1	capacity					856:863	a high adsorption capacity	838:863	a high adsorption capacity of 704 mg/g for iodine vapor	838:892	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	7	20	theme	WCA	1111:1113	arg1	capacity					1087:1094	The adsorption capacity	1072:1094	The adsorption capacity of UiO-66-NH2 @WCA	1072:1113	The adsorption capacity of UiO-66-NH2 @WCA was highest in solution when the pH was 6, while the ionic strength had little effect.					
36332282	7	20	theme	WCA	1111:1113	arg1	highest					1119:1125	highest	1119:1125	highest	1119:1125	The adsorption capacity of UiO-66-NH2 @WCA was highest in solution when the pH was 6, while the ionic strength had little effect.					
36332282	6	21	theme	pseudo-second-order	1037:1055	arg1	model					1065:1069	pseudo-second-order kinetic model	1037:1069	pseudo-second-order kinetic model	1037:1069	The adsorption behavior in iodine aqueous solution was well predicted by the Freundlich isotherm and pseudo-second-order kinetic model.					
36332282	1	22	theme	environmental	165:177	arg1	protection					179:188	environmental protection	165:188	environmental protection	165:188	The capture of radioactive iodine is an inevitable requirement in nuclear industry for environmental protection.					
36332282	3	23	from	drawbacks	371:379	arg1	implementation					411:424	implementation	411:424	implementation	411:424	Although the loading of MOFs on wood can avoid the drawbacks of the powder form of MOFs in implementation, the dense structure of wood results in the lower loading, even after delignification, which limits the adsorption capacity.					
36332282	6	24	from	behavior	951:958	arg1	solution					978:985	iodine aqueous solution	963:985	iodine aqueous solution	963:985	The adsorption behavior in iodine aqueous solution was well predicted by the Freundlich isotherm and pseudo-second-order kinetic model.					
36332282	4	25	attach	removed	716:722	arg2	WCA					694:696	WCA	694:696	WCA	694:696	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	4	25	attach	removed	716:722	arg2	aerogel					685:691	wood-derived cellulose aerogel	662:691	wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood	662:758	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	4	25	attach	removed	716:722	arg1	wood					755:758	delignified wood	743:758	delignified wood	743:758	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	4	26	link	wood-derived	662:673	arg1	aerogel					685:691	wood-derived cellulose aerogel	662:691	wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood	662:758	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	4	26	link	wood-derived	662:673	arg1	WCA					694:696	WCA	694:696	WCA	694:696	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	5	27	theme	UiO-66-NH2	814:823	arg1	crystals					825:832	UiO-66-NH2 crystals	814:832	UiO-66-NH2 crystals	814:832	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	0	28	theme	In	0:1	arg1	growth					8:13	In situ growth	0:13	In situ growth of UiO-66-NH2 in	0:30	In situ growth of UiO-66-NH2 in wood-derived cellulose for iodine adsorption.					
36332282	2	29	contain	have	261:264	arg1	sorbents					247:254	sorbents	247:254	sorbents that have wide applications for iodine adsorption and recovery	247:317	Metal-organic frameworks (MOFs) are a new generation of sorbents that have wide applications for iodine adsorption and recovery.					
36332282	2	29	contain	have	261:264	arg2	applications					271:282	wide applications	266:282	wide applications for iodine adsorption and recovery	266:317	Metal-organic frameworks (MOFs) are a new generation of sorbents that have wide applications for iodine adsorption and recovery.					
36332282	3	30	from	loading	333:339	arg1	wood					352:355	wood	352:355	wood	352:355	Although the loading of MOFs on wood can avoid the drawbacks of the powder form of MOFs in implementation, the dense structure of wood results in the lower loading, even after delignification, which limits the adsorption capacity.					
36332282	3	31	theme	adsorption	530:539	arg1	capacity					541:548	the adsorption capacity	526:548	the adsorption capacity	526:548	Although the loading of MOFs on wood can avoid the drawbacks of the powder form of MOFs in implementation, the dense structure of wood results in the lower loading, even after delignification, which limits the adsorption capacity.					
36332282	3	32	theme	MOFs	403:406	arg1	form					395:398	the powder form	384:398	the powder form of MOFs	384:406	Although the loading of MOFs on wood can avoid the drawbacks of the powder form of MOFs in implementation, the dense structure of wood results in the lower loading, even after delignification, which limits the adsorption capacity.					
36332282	8	33	theme	WCA	1229:1231	arg1	matrix					1233:1238	the WCA matrix	1225:1238	the WCA matrix	1225:1238	The hydroxyl groups on the WCA matrix had a charge transfer effect with iodine, providing additional sites for iodine capture.					
36332282	8	34	theme	charge	1246:1251	arg1	effect					1262:1267	a charge transfer effect	1244:1267	a charge transfer effect	1244:1267	The hydroxyl groups on the WCA matrix had a charge transfer effect with iodine, providing additional sites for iodine capture.					
36332282	3	35	theme	dense	431:435	arg1	structure					437:445	the dense structure	427:445	the dense structure of wood	427:453	Although the loading of MOFs on wood can avoid the drawbacks of the powder form of MOFs in implementation, the dense structure of wood results in the lower loading, even after delignification, which limits the adsorption capacity.					
36332282	5	36	theme	high	789:792	arg1	loading					794:800	a high loading	787:800	a high loading (36 wt%) of UiO-66-NH2 crystals	787:832	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	5	36	theme	high	789:792	arg1	%					808:808	36 wt%	803:808	36 wt%	803:808	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	4	37	theme	delignified	743:753	arg1	wood					755:758	delignified wood	743:758	delignified wood	743:758	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	1	38	theme	radioactive	93:103	arg1	iodine					105:110	radioactive iodine	93:110	radioactive iodine	93:110	The capture of radioactive iodine is an inevitable requirement in nuclear industry for environmental protection.					
36332282	9	39	theme	column	1351:1356	arg1	system					1358:1363	a packed column system	1342:1363	a packed column system	1342:1363	Furthermore, a packed column system was applied to demonstrate the excellent recyclability and potential for practical application.					
36332282	4	40	theme	@	594:594	arg1	composite					599:607	a hierarchically porous UiO-66-NH2 @WCA composite	559:607	a hierarchically porous UiO-66-NH2 @WCA composite	559:607	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	3	41	theme	powder	388:393	arg1	form					395:398	the powder form	384:398	the powder form of MOFs	384:406	Although the loading of MOFs on wood can avoid the drawbacks of the powder form of MOFs in implementation, the dense structure of wood results in the lower loading, even after delignification, which limits the adsorption capacity.					
36332282	8	42	contain	had	1240:1242	arg2	effect					1262:1267	a charge transfer effect	1244:1267	a charge transfer effect	1244:1267	The hydroxyl groups on the WCA matrix had a charge transfer effect with iodine, providing additional sites for iodine capture.					
36332282	8	42	contain	had	1240:1242	arg1	groups					1215:1220	The hydroxyl groups	1202:1220	The hydroxyl groups on the WCA matrix	1202:1238	The hydroxyl groups on the WCA matrix had a charge transfer effect with iodine, providing additional sites for iodine capture.					
36332282	7	43	theme	little	1187:1192	arg1	effect					1194:1199	little effect	1187:1199	little effect	1187:1199	The adsorption capacity of UiO-66-NH2 @WCA was highest in solution when the pH was 6, while the ionic strength had little effect.					
36332282	2	44	theme	new	229:231	arg1	frameworks					205:214	Metal-organic frameworks	191:214	Metal-organic frameworks (MOFs)	191:221	Metal-organic frameworks (MOFs) are a new generation of sorbents that have wide applications for iodine adsorption and recovery.					
36332282	2	44	theme	new	229:231	arg1	generation					233:242	a new generation	227:242	a new generation of sorbents that have wide applications for iodine adsorption and recovery	227:317	Metal-organic frameworks (MOFs) are a new generation of sorbents that have wide applications for iodine adsorption and recovery.					
36332282	4	45	theme	in-situ	627:633	arg1	synthesis					635:643	in-situ synthesis	627:643	in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood	627:758	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	9	46	theme	excellent	1396:1404	arg1	recyclability					1406:1418	the excellent recyclability and potential	1392:1432	recyclability	1406:1418	Furthermore, a packed column system was applied to demonstrate the excellent recyclability and potential for practical application.					
36332282	0	47	theme	UiO-66-NH2	18:27	arg1	growth					8:13	In situ growth	0:13	In situ growth of UiO-66-NH2 in	0:30	In situ growth of UiO-66-NH2 in wood-derived cellulose for iodine adsorption.					
36332282	3	48	theme	form	395:398	arg1	drawbacks					371:379	the drawbacks	367:379	the drawbacks of the powder form of MOFs in implementation	367:424	Although the loading of MOFs on wood can avoid the drawbacks of the powder form of MOFs in implementation, the dense structure of wood results in the lower loading, even after delignification, which limits the adsorption capacity.					
36332282	6	49	theme	iodine	963:968	arg1	solution					978:985	iodine aqueous solution	963:985	iodine aqueous solution	963:985	The adsorption behavior in iodine aqueous solution was well predicted by the Freundlich isotherm and pseudo-second-order kinetic model.					
36332282	8	50	theme	hydroxyl	1206:1213	arg1	groups					1215:1220	The hydroxyl groups	1202:1220	The hydroxyl groups on the WCA matrix	1202:1238	The hydroxyl groups on the WCA matrix had a charge transfer effect with iodine, providing additional sites for iodine capture.					
36332282	5	51	theme	UiO-66-NH2	761:770	arg1	WCA					773:775	UiO-66-NH2 @WCA	761:775	UiO-66-NH2 @WCA	761:775	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	8	52	theme	additional	1292:1301	arg1	sites					1303:1307	additional sites	1292:1307	additional sites for iodine capture	1292:1326	The hydroxyl groups on the WCA matrix had a charge transfer effect with iodine, providing additional sites for iodine capture.					
36332282	5	53	theme	aqueous	918:924	arg1	solution					926:933	iodine aqueous solution	911:933	iodine aqueous solution	911:933	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	1	54	theme	iodine	105:110	arg1	capture					82:88	The capture	78:88	The capture of radioactive iodine	78:110	The capture of radioactive iodine is an inevitable requirement in nuclear industry for environmental protection.					
36332282	1	54	theme	iodine	105:110	arg1	requirement					129:139	an inevitable requirement	115:139	an inevitable requirement in nuclear industry for environmental protection	115:188	The capture of radioactive iodine is an inevitable requirement in nuclear industry for environmental protection.					
36332282	5	55	theme	@	772:772	arg1	WCA					773:775	UiO-66-NH2 @WCA	761:775	UiO-66-NH2 @WCA	761:775	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	4	56	theme	cellulose	675:683	arg1	aerogel					685:691	wood-derived cellulose aerogel	662:691	wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood	662:758	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	4	56	theme	cellulose	675:683	arg1	WCA					694:696	WCA	694:696	WCA	694:696	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	7	57	theme	@	1110:1110	arg1	WCA					1111:1113	UiO-66-NH2 @WCA	1099:1113	UiO-66-NH2 @WCA	1099:1113	The adsorption capacity of UiO-66-NH2 @WCA was highest in solution when the pH was 6, while the ionic strength had little effect.					
36332282	8	58	theme	iodine	1313:1318	arg1	capture					1320:1326	iodine capture	1313:1326	iodine capture	1313:1326	The hydroxyl groups on the WCA matrix had a charge transfer effect with iodine, providing additional sites for iodine capture.					
36332282	5	59	theme	704 mg/g	868:875	arg1	%					808:808	36 wt%	803:808	36 wt%	803:808	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	5	59	theme	704 mg/g	868:875	arg1	loading					794:800	a high loading	787:800	a high loading (36 wt%) of UiO-66-NH2 crystals	787:832	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	5	59	theme	704 mg/g	868:875	arg1	248 mg/g					898:905	248 mg/g	898:905	248 mg/g	898:905	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	5	59	theme	704 mg/g	868:875	arg1	capacity					856:863	a high adsorption capacity	838:863	a high adsorption capacity of 704 mg/g for iodine vapor	838:892	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	5	60	theme	crystals	825:832	arg1	%					808:808	36 wt%	803:808	36 wt%	803:808	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	5	60	theme	crystals	825:832	arg1	loading					794:800	a high loading	787:800	a high loading (36 wt%) of UiO-66-NH2 crystals	787:832	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	5	60	theme	crystals	825:832	arg1	248 mg/g					898:905	248 mg/g	898:905	248 mg/g	898:905	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	5	60	theme	crystals	825:832	arg1	capacity					856:863	a high adsorption capacity	838:863	a high adsorption capacity of 704 mg/g for iodine vapor	838:892	UiO-66-NH2 @WCA exhibited a high loading (36 wt%) of UiO-66-NH2 crystals and a high adsorption capacity of 704 mg/g for iodine vapor and 248 mg/g for iodine aqueous solution.					
36332282	2	61	theme	sorbents	247:254	arg1	frameworks					205:214	Metal-organic frameworks	191:214	Metal-organic frameworks (MOFs)	191:221	Metal-organic frameworks (MOFs) are a new generation of sorbents that have wide applications for iodine adsorption and recovery.					
36332282	2	61	theme	sorbents	247:254	arg1	generation					233:242	a new generation	227:242	a new generation of sorbents that have wide applications for iodine adsorption and recovery	227:317	Metal-organic frameworks (MOFs) are a new generation of sorbents that have wide applications for iodine adsorption and recovery.					
36332282	8	62	from	groups	1215:1220	arg1	matrix					1233:1238	the WCA matrix	1225:1238	the WCA matrix	1225:1238	The hydroxyl groups on the WCA matrix had a charge transfer effect with iodine, providing additional sites for iodine capture.					
36332282	1	63	theme	inevitable	118:127	arg1	capture					82:88	The capture	78:88	The capture of radioactive iodine	78:110	The capture of radioactive iodine is an inevitable requirement in nuclear industry for environmental protection.					
36332282	1	63	theme	inevitable	118:127	arg1	requirement					129:139	an inevitable requirement	115:139	an inevitable requirement in nuclear industry for environmental protection	115:188	The capture of radioactive iodine is an inevitable requirement in nuclear industry for environmental protection.					
36332282	6	64	theme	aqueous	970:976	arg1	solution					978:985	iodine aqueous solution	963:985	iodine aqueous solution	963:985	The adsorption behavior in iodine aqueous solution was well predicted by the Freundlich isotherm and pseudo-second-order kinetic model.					
36332282	0	65	theme	iodine	59:64	arg1	adsorption					66:75	iodine adsorption	59:75	iodine adsorption	59:75	In situ growth of UiO-66-NH2 in wood-derived cellulose for iodine adsorption.					
36332282	2	66	theme	Metal-organic	191:203	arg1	frameworks					205:214	Metal-organic frameworks	191:214	Metal-organic frameworks (MOFs)	191:221	Metal-organic frameworks (MOFs) are a new generation of sorbents that have wide applications for iodine adsorption and recovery.					
36332282	2	66	theme	Metal-organic	191:203	arg1	generation					233:242	a new generation	227:242	a new generation of sorbents that have wide applications for iodine adsorption and recovery	227:317	Metal-organic frameworks (MOFs) are a new generation of sorbents that have wide applications for iodine adsorption and recovery.					
36332282	2	66	theme	Metal-organic	191:203	arg1	MOFs					217:220	MOFs	217:220	MOFs	217:220	Metal-organic frameworks (MOFs) are a new generation of sorbents that have wide applications for iodine adsorption and recovery.					
36332282	4	67	theme	WCA	595:597	arg1	composite					599:607	a hierarchically porous UiO-66-NH2 @WCA composite	559:607	a hierarchically porous UiO-66-NH2 @WCA composite	559:607	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	4	68	theme	UiO-66-NH2	648:657	arg1	synthesis					635:643	in-situ synthesis	627:643	in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood	627:758	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	7	69	theme	ionic	1168:1172	arg1	strength					1174:1181	the ionic strength	1164:1181	the ionic strength	1164:1181	The adsorption capacity of UiO-66-NH2 @WCA was highest in solution when the pH was 6, while the ionic strength had little effect.					
36332282	4	70	theme	UiO-66-NH2	583:592	arg1	composite					599:607	a hierarchically porous UiO-66-NH2 @WCA composite	559:607	a hierarchically porous UiO-66-NH2 @WCA composite	559:607	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
36332282	7	71	theme	UiO-66-NH2	1099:1108	arg1	WCA					1111:1113	UiO-66-NH2 @WCA	1099:1113	UiO-66-NH2 @WCA	1099:1113	The adsorption capacity of UiO-66-NH2 @WCA was highest in solution when the pH was 6, while the ionic strength had little effect.					
36332282	7	72	contain	had	1183:1185	arg1	strength					1174:1181	the ionic strength	1164:1181	the ionic strength	1164:1181	The adsorption capacity of UiO-66-NH2 @WCA was highest in solution when the pH was 6, while the ionic strength had little effect.					
36332282	7	72	contain	had	1183:1185	arg2	effect					1194:1199	little effect	1187:1199	little effect	1187:1199	The adsorption capacity of UiO-66-NH2 @WCA was highest in solution when the pH was 6, while the ionic strength had little effect.					
36332282	0	73	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ growth of UiO-66-NH2 in wood-derived cellulose for iodine adsorption.					
36332282	4	74	theme	porous	576:581	arg1	composite					599:607	a hierarchically porous UiO-66-NH2 @WCA composite	559:607	a hierarchically porous UiO-66-NH2 @WCA composite	559:607	Herein, a hierarchically porous UiO-66-NH2 @WCA composite was fabricated by in-situ synthesis of UiO-66-NH2 in wood-derived cellulose aerogel (WCA) that was further removed hemicellulose from delignified wood.					
35853338	3	0	theme	adsorption	679:688	arg1	high					796:799	high	796:799	high	796:799	Interestingly, the adsorption efficiency of PMAL-CTS/MMT nanocomposite towards both dyes in the single and binary systems was extremely high due to plenty of functional groups.					
35853338	3	0	theme	adsorption	679:688	arg1	efficiency					690:699	the adsorption efficiency	675:699	the adsorption efficiency of PMAL-CTS/MMT nanocomposite towards both dyes in the single and binary systems	675:780	Interestingly, the adsorption efficiency of PMAL-CTS/MMT nanocomposite towards both dyes in the single and binary systems was extremely high due to plenty of functional groups.					
35853338	2	1	theme	surface	581:587	arg1	groups					600:605	surface functional groups	581:605	surface functional groups introduced by poly(maleic acid) and montmorillonite	581:657	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG) was compared to PMAL-CTS, CTS/MMT, and MMT to emphasize the role of surface functional groups introduced by poly(maleic acid) and montmorillonite.					
35853338	4	2	theme	anionic	887:893	arg1	dyes					895:898	cationic and anionic dyes	874:898	cationic and anionic dyes	874:898	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	2	3	theme	brilliant	492:500	arg1	green					502:506	anionic acid yellow-17 (AY17) and cationic brilliant green	449:506	green	502:506	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG) was compared to PMAL-CTS, CTS/MMT, and MMT to emphasize the role of surface functional groups introduced by poly(maleic acid) and montmorillonite.					
35853338	1	4	theme	facile	353:358	arg1	approach					360:367	a facile approach	351:367	a facile approach for adsorption of organic dyes	351:398	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	0	5	theme	single	180:185	arg1	systems					198:204	single and binary systems	180:204	single and binary systems	180:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	4	6	theme	PMAL-CTS/MMT	853:864	arg1	affinity					841:848	The affinity	837:848	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes	837:898	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	0	7	from	dyes	172:175	arg1	systems					198:204	single and binary systems	180:204	single and binary systems	180:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	1	8	link	cross-linked	255:266	arg1	nanospheres					303:313	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres	229:313	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT)	229:328	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	1	8	link	cross-linked	255:266	arg1	PMAL-CTS/MMT					316:327	PMAL-CTS/MMT	316:327	PMAL-CTS/MMT	316:327	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	7	9	contain	has	1660:1662	arg2	potential					1668:1676	the potential to treat concomitant organic dyes effectively	1664:1722	the potential to treat concomitant organic dyes effectively	1664:1722	Collectively, the simple fabrication and superior adsorption performance reveal that PMAL-CTS/MMT has the potential to treat concomitant organic dyes effectively.					
35853338	7	9	contain	has	1660:1662	arg1	PMAL-CTS/MMT					1647:1658	PMAL-CTS/MMT	1647:1658	PMAL-CTS/MMT	1647:1658	Collectively, the simple fabrication and superior adsorption performance reveal that PMAL-CTS/MMT has the potential to treat concomitant organic dyes effectively.					
35853338	4	10	theme	adsorption	1046:1055	arg1	capacity					1057:1064	a maximum adsorption capacity	1036:1064	a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG	1036:1103	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	1	11	theme	-grafted	246:253	arg1	nanospheres					303:313	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres	229:313	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT)	229:328	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	1	11	theme	-grafted	246:253	arg1	PMAL-CTS/MMT					316:327	PMAL-CTS/MMT	316:327	PMAL-CTS/MMT	316:327	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	0	12	theme	ultra-high	95:104	arg1	adsorption					106:115	ultra-high adsorption	95:115	ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems	95:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	3	13	theme	PMAL-CTS/MMT	704:715	arg1	nanocomposite					717:729	PMAL-CTS/MMT nanocomposite	704:729	PMAL-CTS/MMT nanocomposite	704:729	Interestingly, the adsorption efficiency of PMAL-CTS/MMT nanocomposite towards both dyes in the single and binary systems was extremely high due to plenty of functional groups.					
35853338	0	14	theme	binary	191:196	arg1	systems					198:204	single and binary systems	180:204	single and binary systems	180:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	1	15	theme	cross-linked	255:266	arg1	nanospheres					303:313	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres	229:313	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT)	229:328	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	1	15	theme	cross-linked	255:266	arg1	PMAL-CTS/MMT					316:327	PMAL-CTS/MMT	316:327	PMAL-CTS/MMT	316:327	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	0	16	theme	anionic	120:126	arg1	acid					128:131	anionic acid yellow-17	120:141	anionic acid yellow-17	120:141	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	3	17	theme	nanocomposite	717:729	arg1	high					796:799	high	796:799	high	796:799	Interestingly, the adsorption efficiency of PMAL-CTS/MMT nanocomposite towards both dyes in the single and binary systems was extremely high due to plenty of functional groups.					
35853338	3	17	theme	nanocomposite	717:729	arg1	efficiency					690:699	the adsorption efficiency	675:699	the adsorption efficiency of PMAL-CTS/MMT nanocomposite towards both dyes in the single and binary systems	675:780	Interestingly, the adsorption efficiency of PMAL-CTS/MMT nanocomposite towards both dyes in the single and binary systems was extremely high due to plenty of functional groups.					
35853338	4	18	dep	exhibited	1026:1034	arg1	higher					1129:1134	higher	1129:1134	higher	1129:1134	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	4	19	theme	1910 mg g-1	1077:1087	arg1	capacity					1057:1064	a maximum adsorption capacity	1036:1064	a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG	1036:1103	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	2	20	theme	adsorption	405:414	arg1	capacity					416:423	The adsorption capacity	401:423	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG)	401:511	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG) was compared to PMAL-CTS, CTS/MMT, and MMT to emphasize the role of surface functional groups introduced by poly(maleic acid) and montmorillonite.					
35853338	4	21	dep	nanocomposite	1012:1024	arg1	pH.					991:993	pH.	991:993	pH.	991:993	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	4	22	theme	recent	1176:1181	arg1	studies					1194:1200	recent literature studies	1176:1200	recent literature studies	1176:1200	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	6	23	from	environments	1548:1559	arg1	separate					1495:1502	separate	1495:1502	separate	1495:1502	More importantly, the PMAL-CTS/MMT nanocomposite was successfully applied to separate the AY17 and BG dyes from real-life aquatic environments.					
35853338	6	24	theme	aquatic	1540:1546	arg1	environments					1548:1559	real-life aquatic environments	1530:1559	real-life aquatic environments	1530:1559	More importantly, the PMAL-CTS/MMT nanocomposite was successfully applied to separate the AY17 and BG dyes from real-life aquatic environments.					
35853338	4	25	theme	surface	945:951	arg1	charges					953:959	the surface charges	941:959	the surface charges	941:959	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	4	26	theme	PMAL-CTS/MMT	999:1010	arg1	nanocomposite					1012:1024	The PMAL-CTS/MMT nanocomposite	995:1024	The PMAL-CTS/MMT nanocomposite	995:1024	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	1	27	theme	organic	387:393	arg1	dyes					395:398	organic dyes	387:398	organic dyes	387:398	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	2	28	theme	groups	600:605	arg1	role					573:576	the role	569:576	the role of surface functional groups introduced by poly(maleic acid) and montmorillonite	569:657	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG) was compared to PMAL-CTS, CTS/MMT, and MMT to emphasize the role of surface functional groups introduced by poly(maleic acid) and montmorillonite.					
35853338	1	29	theme	chitosan/montmorillonite	268:291	arg1	nanospheres					303:313	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres	229:313	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT)	229:328	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	1	29	theme	chitosan/montmorillonite	268:291	arg1	PMAL-CTS/MMT					316:327	PMAL-CTS/MMT	316:327	PMAL-CTS/MMT	316:327	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	4	30	theme	cationic	874:881	arg1	dyes					895:898	cationic and anionic dyes	874:898	cationic and anionic dyes	874:898	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	6	31	theme	real-life	1530:1538	arg1	environments					1548:1559	real-life aquatic environments	1530:1559	real-life aquatic environments	1530:1559	More importantly, the PMAL-CTS/MMT nanocomposite was successfully applied to separate the AY17 and BG dyes from real-life aquatic environments.					
35853338	4	32	theme	feasible	918:925	arg1	modulation					927:936	the feasible modulation	914:936	the feasible modulation of the surface charges	914:959	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	0	33	from	systems	198:204	arg1	adsorption					106:115	ultra-high adsorption	95:115	ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems	95:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	2	34	theme	functional	589:598	arg1	groups					600:605	surface functional groups	581:605	surface functional groups introduced by poly(maleic acid) and montmorillonite	581:657	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG) was compared to PMAL-CTS, CTS/MMT, and MMT to emphasize the role of surface functional groups introduced by poly(maleic acid) and montmorillonite.					
35853338	2	35	theme	maleic	626:631	arg1	poly					621:624	poly	621:624	poly(maleic acid)	621:637	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG) was compared to PMAL-CTS, CTS/MMT, and MMT to emphasize the role of surface functional groups introduced by poly(maleic acid) and montmorillonite.					
35853338	2	35	theme	maleic	626:631	arg1	acid					633:636	maleic acid	626:636	maleic acid	626:636	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG) was compared to PMAL-CTS, CTS/MMT, and MMT to emphasize the role of surface functional groups introduced by poly(maleic acid) and montmorillonite.					
35853338	7	36	theme	organic	1699:1705	arg1	dyes					1707:1710	concomitant organic dyes	1687:1710	concomitant organic dyes	1687:1710	Collectively, the simple fabrication and superior adsorption performance reveal that PMAL-CTS/MMT has the potential to treat concomitant organic dyes effectively.					
35853338	1	37	theme	composite	293:301	arg1	nanospheres					303:313	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres	229:313	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT)	229:328	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	1	37	theme	composite	293:301	arg1	PMAL-CTS/MMT					316:327	PMAL-CTS/MMT	316:327	PMAL-CTS/MMT	316:327	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	4	38	theme	literature	1183:1192	arg1	studies					1194:1200	recent literature studies	1176:1200	recent literature studies	1176:1200	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	0	39	theme	poly	15:18	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of poly(maleic acid)	0:31	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	0	40	theme	acid	128:131	arg1	adsorption					106:115	ultra-high adsorption	95:115	ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems	95:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	1	41	theme	dyes	395:398	arg1	adsorption					373:382	adsorption	373:382	adsorption of organic dyes	373:398	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	6	42	theme	BG	1517:1518	arg1	dyes					1520:1523	the AY17 and BG dyes	1504:1523	the AY17 and BG dyes	1504:1523	More importantly, the PMAL-CTS/MMT nanocomposite was successfully applied to separate the AY17 and BG dyes from real-life aquatic environments.					
35853338	2	43	theme	PMAL-CTS/MMT	428:439	arg1	capacity					416:423	The adsorption capacity	401:423	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG)	401:511	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG) was compared to PMAL-CTS, CTS/MMT, and MMT to emphasize the role of surface functional groups introduced by poly(maleic acid) and montmorillonite.					
35853338	2	44	theme	cationic	483:490	arg1	green					502:506	anionic acid yellow-17 (AY17) and cationic brilliant green	449:506	green	502:506	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG) was compared to PMAL-CTS, CTS/MMT, and MMT to emphasize the role of surface functional groups introduced by poly(maleic acid) and montmorillonite.					
35853338	5	45	theme	electrostatic	1349:1361	arg1	interaction					1363:1373	electrostatic interaction	1349:1373	electrostatic interaction	1349:1373	The proposed mechanism based on the characterization of PMAL-CTS/MMT after the adsorption highlighted that the adsorption is mainly controlled by electrostatic interaction, π - π interactions, and hydrogen bonding.					
35853338	0	46	theme	cationic	147:154	arg1	dyes					172:175	cationic brilliant green dyes	147:175	cationic brilliant green dyes in single and binary systems	147:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	7	47	theme	concomitant	1687:1697	arg1	dyes					1707:1710	concomitant organic dyes	1687:1710	concomitant organic dyes	1687:1710	Collectively, the simple fabrication and superior adsorption performance reveal that PMAL-CTS/MMT has the potential to treat concomitant organic dyes effectively.					
35853338	0	48	theme	maleic	20:25	arg1	poly					15:18	poly	15:18	poly(maleic acid)	15:31	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	0	48	theme	maleic	20:25	arg1	acid					27:30	maleic acid	20:30	maleic acid	20:30	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	7	49	theme	simple	1580:1585	arg1	fabrication					1587:1597	the simple fabrication	1576:1597	the simple fabrication	1576:1597	Collectively, the simple fabrication and superior adsorption performance reveal that PMAL-CTS/MMT has the potential to treat concomitant organic dyes effectively.					
35853338	5	50	theme	π - π	1376:1380	arg1	interactions					1382:1393	π - π interactions	1376:1393	π - π interactions	1376:1393	The proposed mechanism based on the characterization of PMAL-CTS/MMT after the adsorption highlighted that the adsorption is mainly controlled by electrostatic interaction, π - π interactions, and hydrogen bonding.					
35853338	7	51	theme	adsorption	1612:1621	arg1	performance					1623:1633	superior adsorption performance	1603:1633	superior adsorption performance	1603:1633	Collectively, the simple fabrication and superior adsorption performance reveal that PMAL-CTS/MMT has the potential to treat concomitant organic dyes effectively.					
35853338	3	52	theme	binary	767:772	arg1	systems					774:780	the single and binary systems	752:780	the single and binary systems	752:780	Interestingly, the adsorption efficiency of PMAL-CTS/MMT nanocomposite towards both dyes in the single and binary systems was extremely high due to plenty of functional groups.					
35853338	0	53	theme	green	166:170	arg1	dyes					172:175	cationic brilliant green dyes	147:175	cationic brilliant green dyes in single and binary systems	147:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	4	54	theme	charges	953:959	arg1	modulation					927:936	the feasible modulation	914:936	the feasible modulation of the surface charges	914:959	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	4	55	theme	solution	982:989	arg1	function					966:973	a function	964:973	a function of the solution pH. The PMAL-CTS/MMT nanocomposite	964:1024	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	0	56	theme	brilliant	156:164	arg1	dyes					172:175	cationic brilliant green dyes	147:175	cationic brilliant green dyes in single and binary systems	147:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	4	57	theme	518	1069:1071	arg1	capacity					1057:1064	a maximum adsorption capacity	1036:1064	a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG	1036:1103	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	3	58	theme	single	756:761	arg1	systems					774:780	the single and binary systems	752:780	the single and binary systems	752:780	Interestingly, the adsorption efficiency of PMAL-CTS/MMT nanocomposite towards both dyes in the single and binary systems was extremely high due to plenty of functional groups.					
35853338	5	59	theme	hydrogen	1400:1407	arg1	bonding					1409:1415	hydrogen bonding	1400:1415	hydrogen bonding	1400:1415	The proposed mechanism based on the characterization of PMAL-CTS/MMT after the adsorption highlighted that the adsorption is mainly controlled by electrostatic interaction, π - π interactions, and hydrogen bonding.					
35853338	0	60	link	cross-linked	41:52	arg1	nanospheres					79:89	cross-linked chitosan/montmorillonite nanospheres	41:89	cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems	41:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	5	61	theme	PMAL-CTS/MMT	1259:1270	arg1	characterization					1239:1254	the characterization	1235:1254	the characterization of PMAL-CTS/MMT after the adsorption highlighted that the adsorption	1235:1323	The proposed mechanism based on the characterization of PMAL-CTS/MMT after the adsorption highlighted that the adsorption is mainly controlled by electrostatic interaction, π - π interactions, and hydrogen bonding.					
35853338	7	62	theme	superior	1603:1610	arg1	performance					1623:1633	superior adsorption performance	1603:1633	superior adsorption performance	1603:1633	Collectively, the simple fabrication and superior adsorption performance reveal that PMAL-CTS/MMT has the potential to treat concomitant organic dyes effectively.					
35853338	0	63	theme	dyes	172:175	arg1	adsorption					106:115	ultra-high adsorption	95:115	ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems	95:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	0	64	from	adsorption	106:115	arg1	systems					198:204	single and binary systems	180:204	single and binary systems	180:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	3	65	theme	functional	818:827	arg1	groups					829:834	functional groups	818:834	functional groups	818:834	Interestingly, the adsorption efficiency of PMAL-CTS/MMT nanocomposite towards both dyes in the single and binary systems was extremely high due to plenty of functional groups.					
35853338	1	66	theme	poly	229:232	arg1	nanospheres					303:313	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres	229:313	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT)	229:328	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	1	66	theme	poly	229:232	arg1	PMAL-CTS/MMT					316:327	PMAL-CTS/MMT	316:327	PMAL-CTS/MMT	316:327	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	0	67	theme	chitosan/montmorillonite	54:77	arg1	nanospheres					79:89	cross-linked chitosan/montmorillonite nanospheres	41:89	cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems	41:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	3	68	from	dyes	744:747	arg1	systems					774:780	the single and binary systems	752:780	the single and binary systems	752:780	Interestingly, the adsorption efficiency of PMAL-CTS/MMT nanocomposite towards both dyes in the single and binary systems was extremely high due to plenty of functional groups.					
35853338	2	69	theme	anionic	449:455	arg1	BG					509:510	BG	509:510	BG	509:510	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG) was compared to PMAL-CTS, CTS/MMT, and MMT to emphasize the role of surface functional groups introduced by poly(maleic acid) and montmorillonite.					
35853338	2	69	theme	anionic	449:455	arg1	acid					457:460	anionic acid yellow-17 (AY17) and cationic brilliant green	449:506	acid	457:460	The adsorption capacity of PMAL-CTS/MMT towards anionic acid yellow-17 (AY17) and cationic brilliant green (BG) was compared to PMAL-CTS, CTS/MMT, and MMT to emphasize the role of surface functional groups introduced by poly(maleic acid) and montmorillonite.					
35853338	3	70	theme	groups	829:834	arg1	plenty					808:813	plenty	808:813	plenty of functional groups	808:834	Interestingly, the adsorption efficiency of PMAL-CTS/MMT nanocomposite towards both dyes in the single and binary systems was extremely high due to plenty of functional groups.					
35853338	5	71	theme	proposed	1207:1214	arg1	mechanism					1216:1224	The proposed mechanism	1203:1224	The proposed mechanism based on the characterization of PMAL-CTS/MMT after the adsorption highlighted that the adsorption	1203:1323	The proposed mechanism based on the characterization of PMAL-CTS/MMT after the adsorption highlighted that the adsorption is mainly controlled by electrostatic interaction, π - π interactions, and hydrogen bonding.					
35853338	0	72	theme	cross-linked	41:52	arg1	nanospheres					79:89	cross-linked chitosan/montmorillonite nanospheres	41:89	cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems	41:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
35853338	6	73	theme	AY17	1508:1511	arg1	dyes					1520:1523	the AY17 and BG dyes	1504:1523	the AY17 and BG dyes	1504:1523	More importantly, the PMAL-CTS/MMT nanocomposite was successfully applied to separate the AY17 and BG dyes from real-life aquatic environments.					
35853338	1	74	theme	maleic	234:239	arg1	poly					229:232	poly	229:232	poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT)	229:328	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	1	74	theme	maleic	234:239	arg1	acid					241:244	maleic acid	234:244	maleic acid	234:244	In this contribution, poly(maleic acid)-grafted cross-linked chitosan/montmorillonite composite nanospheres (PMAL-CTS/MMT) were synthesized via a facile approach for adsorption of organic dyes.					
35853338	6	75	theme	PMAL-CTS/MMT	1440:1451	arg1	nanocomposite					1453:1465	the PMAL-CTS/MMT nanocomposite	1436:1465	the PMAL-CTS/MMT nanocomposite	1436:1465	More importantly, the PMAL-CTS/MMT nanocomposite was successfully applied to separate the AY17 and BG dyes from real-life aquatic environments.					
35853338	4	76	theme	maximum	1038:1044	arg1	capacity					1057:1064	a maximum adsorption capacity	1036:1064	a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG	1036:1103	The affinity of PMAL-CTS/MMT towards cationic and anionic dyes resulted from the feasible modulation of the surface charges as a function of the solution pH. The PMAL-CTS/MMT nanocomposite exhibited a maximum adsorption capacity of 518 and 1910 mg g-1 for AY17 and BG, respectively, which is higher than most of the adsorbents reported in recent literature studies.					
35853338	0	77	from	acid	128:131	arg1	systems					198:204	single and binary systems	180:204	single and binary systems	180:204	Fabrication of poly(maleic acid)-grafted cross-linked chitosan/montmorillonite nanospheres for ultra-high adsorption of anionic acid yellow-17 and cationic brilliant green dyes in single and binary systems.					
36493927	5	0	theme	composite	920:928	arg1	film					930:933	the composite film	916:933	the composite film	916:933	More importantly, in addition to extending the shelf life of cherry tomatoes, the composite film is also biodegradable to some degree.					
36493927	5	0	theme	composite	920:928	arg1	biodegradable					943:955	biodegradable	943:955	biodegradable	943:955	More importantly, in addition to extending the shelf life of cherry tomatoes, the composite film is also biodegradable to some degree.					
36493927	6	1	theme	packaging	1097:1105	arg1	applications					1107:1118	food packaging applications	1092:1118	food packaging applications	1092:1118	Therefore, polyvinyl alcohol films based on ACS and ACS-ZnO NPs added as antimicrobial agents have great potential for food packaging applications.					
36493927	4	2	theme	excellent	772:780	arg1	activities					796:805	excellent antibacterial activities	772:805	excellent antibacterial activities against S. aureus and E. coli	772:835	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	3	3	theme	NPs	568:570	arg1	experiments					537:547	The cytotoxicity experiments	520:547	The cytotoxicity experiments of ACS and ACS-ZnO NPs on A549 cells	520:584	The cytotoxicity experiments of ACS and ACS-ZnO NPs on A549 cells showed that both had good cytocompatibility.					
36493927	1	4	theme	soft	140:143	arg1	membrane					151:158	a soft brain membrane and chelating agent	138:178	membrane	151:158	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	1	4	theme	soft	140:143	arg1	chitosan					111:118	arginineated chitosan	98:118	arginineated chitosan (ACS)	98:124	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	1	5	theme	ACS-ZnO	224:230	arg1	NPs					232:234	ACS-ZnO NPs	224:234	ACS-ZnO NPs	224:234	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	1	6	theme	antimicrobial	289:301	arg1	agent					303:307	an antimicrobial agent	286:307	an antimicrobial agent to form films by casting	286:332	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	4	7	theme	oxygen	744:749	arg1	properties					713:722	the composite film's mechanical properties	681:722	the composite film's mechanical properties	681:722	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	4	7	theme	oxygen	744:749	arg1	barrier					751:757	oxygen barrier	744:757	oxygen barrier	744:757	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	6	8	theme	ACS-ZnO	1025:1031	arg1	NPs					1033:1035	ACS-ZnO NPs	1025:1035	ACS-ZnO NPs	1025:1035	Therefore, polyvinyl alcohol films based on ACS and ACS-ZnO NPs added as antimicrobial agents have great potential for food packaging applications.					
36493927	1	9	theme	brain	145:149	arg1	membrane					151:158	a soft brain membrane and chelating agent	138:178	membrane	151:158	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	1	9	theme	brain	145:149	arg1	chitosan					111:118	arginineated chitosan	98:118	arginineated chitosan (ACS)	98:124	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	2	10	theme	1H	434:435	arg1	NMR					437:439	1H NMR	434:439	1H NMR	434:439	The formation and structural morphology of ACS and ACS-ZnO NPs were investigated by applying FTIR, 1H NMR, XRD, EDS, SEM, and TEM techniques, and ACS has shown better water solubility.					
36493927	2	11	theme	water	502:506	arg1	solubility					508:517	better water solubility	495:517	better water solubility	495:517	The formation and structural morphology of ACS and ACS-ZnO NPs were investigated by applying FTIR, 1H NMR, XRD, EDS, SEM, and TEM techniques, and ACS has shown better water solubility.					
36493927	1	12	used	used	130:133	arg2	chitosan					111:118	arginineated chitosan	98:118	arginineated chitosan (ACS)	98:124	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	1	12	used	used	130:133	arg2	ACS					121:123	ACS	121:123	ACS	121:123	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	1	12	used	used	130:133	arg2	agent					174:178	a soft brain membrane and chelating agent	138:178	agent	174:178	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	1	12	used	used	130:133	arg2	membrane					151:158	a soft brain membrane and chelating agent	138:178	membrane	151:158	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	2	13	theme	better	495:500	arg1	solubility					508:517	better water solubility	495:517	better water solubility	495:517	The formation and structural morphology of ACS and ACS-ZnO NPs were investigated by applying FTIR, 1H NMR, XRD, EDS, SEM, and TEM techniques, and ACS has shown better water solubility.					
36493927	2	14	theme	ACS	378:380	arg1	formation					339:347	formation	339:347	formation	339:347	The formation and structural morphology of ACS and ACS-ZnO NPs were investigated by applying FTIR, 1H NMR, XRD, EDS, SEM, and TEM techniques, and ACS has shown better water solubility.					
36493927	2	14	theme	ACS	378:380	arg1	morphology					364:373	structural morphology	353:373	structural morphology	353:373	The formation and structural morphology of ACS and ACS-ZnO NPs were investigated by applying FTIR, 1H NMR, XRD, EDS, SEM, and TEM techniques, and ACS has shown better water solubility.					
36493927	4	15	theme	water	725:729	arg1	properties					713:722	the composite film's mechanical properties	681:722	the composite film's mechanical properties	681:722	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	4	15	theme	water	725:729	arg1	barrier					731:737	water barrier	725:737	water barrier	725:737	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	1	16	theme	chelating	164:172	arg1	agent					174:178	a soft brain membrane and chelating agent	138:178	agent	174:178	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	1	16	theme	chelating	164:172	arg1	chitosan					111:118	arginineated chitosan	98:118	arginineated chitosan (ACS)	98:124	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	6	17	theme	great	1072:1076	arg1	potential					1078:1086	great potential	1072:1086	great potential for food packaging applications	1072:1118	Therefore, polyvinyl alcohol films based on ACS and ACS-ZnO NPs added as antimicrobial agents have great potential for food packaging applications.					
36493927	1	18	theme	arginineated	98:109	arg1	ACS					121:123	ACS	121:123	ACS	121:123	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	1	18	theme	arginineated	98:109	arg1	membrane					151:158	a soft brain membrane and chelating agent	138:178	membrane	151:158	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	1	18	theme	arginineated	98:109	arg1	agent					174:178	a soft brain membrane and chelating agent	138:178	agent	174:178	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	1	18	theme	arginineated	98:109	arg1	chitosan					111:118	arginineated chitosan	98:118	arginineated chitosan (ACS)	98:124	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	5	19	theme	shelf	885:889	arg1	life					891:894	the shelf life	881:894	the shelf life of cherry tomatoes	881:913	More importantly, in addition to extending the shelf life of cherry tomatoes, the composite film is also biodegradable to some degree.					
36493927	2	20	theme	structural	353:362	arg1	morphology					364:373	structural morphology	353:373	structural morphology	353:373	The formation and structural morphology of ACS and ACS-ZnO NPs were investigated by applying FTIR, 1H NMR, XRD, EDS, SEM, and TEM techniques, and ACS has shown better water solubility.					
36493927	3	21	theme	good	607:610	arg1	cytocompatibility					612:628	good cytocompatibility	607:628	good cytocompatibility	607:628	The cytotoxicity experiments of ACS and ACS-ZnO NPs on A549 cells showed that both had good cytocompatibility.					
36493927	3	22	theme	cytotoxicity	524:535	arg1	experiments					537:547	The cytotoxicity experiments	520:547	The cytotoxicity experiments of ACS and ACS-ZnO NPs on A549 cells	520:584	The cytotoxicity experiments of ACS and ACS-ZnO NPs on A549 cells showed that both had good cytocompatibility.					
36493927	0	23	theme	chitosan/ZnO	49:60	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of PVA/arginine chitosan/ZnO NPs composite films.					
36493927	0	23	theme	chitosan/ZnO	49:60	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of PVA/arginine chitosan/ZnO NPs composite films.					
36493927	2	24	dep	formation	339:347	arg1	The					335:337	The	335:337	The	335:337	The formation and structural morphology of ACS and ACS-ZnO NPs were investigated by applying FTIR, 1H NMR, XRD, EDS, SEM, and TEM techniques, and ACS has shown better water solubility.					
36493927	6	25	theme	food	1092:1095	arg1	applications					1107:1118	food packaging applications	1092:1118	food packaging applications	1092:1118	Therefore, polyvinyl alcohol films based on ACS and ACS-ZnO NPs added as antimicrobial agents have great potential for food packaging applications.					
36493927	0	26	theme	PVA/arginine	36:47	arg1	chitosan/ZnO					49:60	PVA/arginine chitosan/ZnO	36:60	PVA/arginine chitosan/ZnO	36:60	Preparation and characterization of PVA/arginine chitosan/ZnO NPs composite films.					
36493927	1	27	theme	polyvinyl	252:260	arg1	alcohol					262:268	polyvinyl alcohol	252:268	a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting	250:332	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	1	27	theme	polyvinyl	252:260	arg1	PVA					271:273	PVA	271:273	PVA	271:273	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	4	28	theme	composite	685:693	arg1	film					695:698	the composite film's	681:700	the composite film's mechanical properties	681:722	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	1	29	theme	ACS-ZnO	194:200	arg1	NPs					202:204	ACS-ZnO NPs	194:204	ACS-ZnO NPs	194:204	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	2	30	theme	NPs	394:396	arg1	formation					339:347	formation	339:347	formation	339:347	The formation and structural morphology of ACS and ACS-ZnO NPs were investigated by applying FTIR, 1H NMR, XRD, EDS, SEM, and TEM techniques, and ACS has shown better water solubility.					
36493927	2	30	theme	NPs	394:396	arg1	morphology					364:373	structural morphology	353:373	structural morphology	353:373	The formation and structural morphology of ACS and ACS-ZnO NPs were investigated by applying FTIR, 1H NMR, XRD, EDS, SEM, and TEM techniques, and ACS has shown better water solubility.					
36493927	1	31	theme	alcohol	262:268	arg1	matrix					276:281	a polyvinyl alcohol (PVA) matrix	250:281	a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting	250:332	In this study, arginineated chitosan (ACS) was used as a soft brain membrane and chelating agent to synthesize ACS-ZnO NPs, and then ACS and ACS-ZnO NPs were added to a polyvinyl alcohol (PVA) matrix as an antimicrobial agent to form films by casting.					
36493927	4	32	theme	mechanical	702:711	arg1	properties					713:722	the composite film's mechanical properties	681:722	the composite film's mechanical properties	681:722	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	4	32	theme	mechanical	702:711	arg1	barrier					731:737	water barrier	725:737	water barrier	725:737	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	4	32	theme	mechanical	702:711	arg1	barrier					751:757	oxygen barrier	744:757	oxygen barrier	744:757	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	6	33	theme	alcohol	994:1000	arg1	films					1002:1006	polyvinyl alcohol films	984:1006	polyvinyl alcohol films based on ACS and ACS-ZnO NPs added as antimicrobial agents	984:1065	Therefore, polyvinyl alcohol films based on ACS and ACS-ZnO NPs added as antimicrobial agents have great potential for food packaging applications.					
36493927	2	34	theme	ACS-ZnO	386:392	arg1	NPs					394:396	ACS-ZnO NPs	386:396	ACS-ZnO NPs	386:396	The formation and structural morphology of ACS and ACS-ZnO NPs were investigated by applying FTIR, 1H NMR, XRD, EDS, SEM, and TEM techniques, and ACS has shown better water solubility.					
36493927	2	35	theme	TEM	461:463	arg1	techniques					465:474	TEM techniques	461:474	TEM techniques	461:474	The formation and structural morphology of ACS and ACS-ZnO NPs were investigated by applying FTIR, 1H NMR, XRD, EDS, SEM, and TEM techniques, and ACS has shown better water solubility.					
36493927	5	36	theme	tomatoes	906:913	arg1	life					891:894	the shelf life	881:894	the shelf life of cherry tomatoes	881:913	More importantly, in addition to extending the shelf life of cherry tomatoes, the composite film is also biodegradable to some degree.					
36493927	5	37	theme	cherry	899:904	arg1	tomatoes					906:913	cherry tomatoes	899:913	cherry tomatoes	899:913	More importantly, in addition to extending the shelf life of cherry tomatoes, the composite film is also biodegradable to some degree.					
36493927	6	38	theme	polyvinyl	984:992	arg1	alcohol					994:1000	polyvinyl alcohol	984:1000	polyvinyl alcohol films based on ACS and ACS-ZnO NPs added as antimicrobial agents	984:1065	Therefore, polyvinyl alcohol films based on ACS and ACS-ZnO NPs added as antimicrobial agents have great potential for food packaging applications.					
36493927	3	39	from	experiments	537:547	arg1	cells					580:584	A549 cells	575:584	A549 cells	575:584	The cytotoxicity experiments of ACS and ACS-ZnO NPs on A549 cells showed that both had good cytocompatibility.					
36493927	6	40	contain	have	1067:1070	arg1	films					1002:1006	polyvinyl alcohol films	984:1006	polyvinyl alcohol films based on ACS and ACS-ZnO NPs added as antimicrobial agents	984:1065	Therefore, polyvinyl alcohol films based on ACS and ACS-ZnO NPs added as antimicrobial agents have great potential for food packaging applications.					
36493927	6	40	contain	have	1067:1070	arg2	potential					1078:1086	great potential	1072:1086	great potential for food packaging applications	1072:1118	Therefore, polyvinyl alcohol films based on ACS and ACS-ZnO NPs added as antimicrobial agents have great potential for food packaging applications.					
36493927	4	41	theme	antibacterial	782:794	arg1	activities					796:805	excellent antibacterial activities	772:805	excellent antibacterial activities against S. aureus and E. coli	772:835	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	3	42	theme	ACS-ZnO	560:566	arg1	NPs					568:570	ACS-ZnO NPs	560:570	ACS-ZnO NPs	560:570	The cytotoxicity experiments of ACS and ACS-ZnO NPs on A549 cells showed that both had good cytocompatibility.					
36493927	4	43	theme	NPs	668:670	arg1	incorporation					635:647	The incorporation	631:647	The incorporation of ACS and ACS-ZnO NPs	631:670	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	3	44	contain	had	603:605	arg1	both					598:601	both	598:601	both	598:601	The cytotoxicity experiments of ACS and ACS-ZnO NPs on A549 cells showed that both had good cytocompatibility.					
36493927	3	44	contain	had	603:605	arg2	cytocompatibility					612:628	good cytocompatibility	607:628	good cytocompatibility	607:628	The cytotoxicity experiments of ACS and ACS-ZnO NPs on A549 cells showed that both had good cytocompatibility.					
36493927	4	45	theme	ACS	652:654	arg1	NPs					668:670	ACS and ACS-ZnO NPs	652:670	ACS and ACS-ZnO NPs	652:670	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	4	46	theme	ACS-ZnO	660:666	arg1	NPs					668:670	ACS and ACS-ZnO NPs	652:670	ACS and ACS-ZnO NPs	652:670	The incorporation of ACS and ACS-ZnO NPs improves the composite film's mechanical properties, water barrier, and oxygen barrier and exhibits excellent antibacterial activities against S. aureus and E. coli.					
36493927	3	47	theme	ACS	552:554	arg1	experiments					537:547	The cytotoxicity experiments	520:547	The cytotoxicity experiments of ACS and ACS-ZnO NPs on A549 cells	520:584	The cytotoxicity experiments of ACS and ACS-ZnO NPs on A549 cells showed that both had good cytocompatibility.					
36493927	6	48	theme	antimicrobial	1046:1058	arg1	agents					1060:1065	antimicrobial agents	1046:1065	antimicrobial agents	1046:1065	Therefore, polyvinyl alcohol films based on ACS and ACS-ZnO NPs added as antimicrobial agents have great potential for food packaging applications.					
36493927	5	49	dep	extending	871:879	arg1	addition					859:866	addition	859:866	addition	859:866	More importantly, in addition to extending the shelf life of cherry tomatoes, the composite film is also biodegradable to some degree.					
36493927	0	50	theme	composite	66:74	arg1	films					76:80	composite films	66:80	composite films	66:80	Preparation and characterization of PVA/arginine chitosan/ZnO NPs composite films.					
36493927	3	51	theme	A549	575:578	arg1	cells					580:584	A549 cells	575:584	A549 cells	575:584	The cytotoxicity experiments of ACS and ACS-ZnO NPs on A549 cells showed that both had good cytocompatibility.					
36906363	0	0	theme	fruit	92:96	arg1	drop					98:101	citrus physiological premature fruit drop	61:101	citrus physiological premature fruit drop	61:101	Structural characteristics and gel properties of pectin from citrus physiological premature fruit drop.					
36906363	4	1	theme	composition	465:475	arg1	results					497:503	The monosaccharide composition and molar mass test results	446:503	The monosaccharide composition and molar mass test results	446:503	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	2	dep	showed	505:510	arg1	chain					688:692	galactose side chain	673:692	galactose side chain (32.02 %)	673:702	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	2	dep	showed	505:510	arg1	%					701:701	32.02 %	695:701	32.02 %	695:701	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	2	dep	showed	505:510	arg1	β					570:570	β	570:570	β	570:570	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	2	dep	showed	505:510	arg1	polysaccharide					554:567	a highly branched macromolecular polysaccharide	521:567	a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose	521:667	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	0	3	theme	premature	82:90	arg1	drop					98:101	citrus physiological premature fruit drop	61:101	citrus physiological premature fruit drop	61:101	Structural characteristics and gel properties of pectin from citrus physiological premature fruit drop.					
36906363	4	4	theme	mass	487:490	arg1	results					497:503	The monosaccharide composition and molar mass test results	446:503	The monosaccharide composition and molar mass test results	446:503	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	5	theme	macromolecular	539:552	arg1	β					570:570	β	570:570	β	570:570	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	5	theme	macromolecular	539:552	arg1	polysaccharide					554:567	a highly branched macromolecular polysaccharide	521:567	a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose	521:667	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	3	6	theme	premature	349:357	arg1	CPDP					378:381	CPDP	378:381	CPDP	378:381	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	3	6	theme	premature	349:357	arg1	pectin					370:375	citrus physiological premature fruit drop pectin	328:375	citrus physiological premature fruit drop pectin (CPDP)	328:382	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	4	7	theme	molar	481:485	arg1	mass					487:490	molar mass	481:490	molar mass	481:490	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	5	8	used	used	750:753	arg2	Ca2+					741:744	Ca2+	741:744	Ca2+	741:744	Based on the fact that CPDP is LMP, Ca2+ was used to induce CPDP to form gels.					
36906363	3	9	theme	fruit	359:363	arg1	CPDP					378:381	CPDP	378:381	CPDP	378:381	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	3	9	theme	fruit	359:363	arg1	pectin					370:375	citrus physiological premature fruit drop pectin	328:375	citrus physiological premature fruit drop pectin (CPDP)	328:382	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	6	10	theme	Textural	784:791	arg1	tests					809:813	Textural and rheological tests	784:813	Textural and rheological tests	784:813	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	6	11	theme	lower	949:953	arg1	DM					955:956	the lower DM	945:956	the lower DM	945:956	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	6	12	theme	CPDP	867:870	arg1	strength					835:842	gel strength	831:842	gel strength	831:842	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	6	12	theme	CPDP	867:870	arg1	modulus					856:862	storage modulus	848:862	storage modulus	848:862	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	3	13	theme	citrus	328:333	arg1	CPDP					378:381	CPDP	378:381	CPDP	378:381	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	3	13	theme	citrus	328:333	arg1	pectin					370:375	citrus physiological premature fruit drop pectin	328:375	citrus physiological premature fruit drop pectin (CPDP)	328:382	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	6	14	theme	side	981:984	arg1	chains					986:991	rich neutral sugar side chains	962:991	rich neutral sugar side chains of CPDP	962:999	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	2	15	theme	hydrolysis	264:273	arg1	method					275:280	acid hydrolysis method	259:280	acid hydrolysis method	259:280	The extraction yield of pectin reached 4.4 % by acid hydrolysis method.					
36906363	4	16	theme	rhamnogalacturonan	612:629	arg1	%					647:647	50.40 %	641:647	50.40 %	641:647	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	16	theme	rhamnogalacturonan	612:629	arg1	domain					633:638	rich rhamnogalacturonan I domain	607:638	rich rhamnogalacturonan I domain (50.40 %)	607:648	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	3	17	theme	physiological	335:347	arg1	CPDP					378:381	CPDP	378:381	CPDP	378:381	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	3	17	theme	physiological	335:347	arg1	pectin					370:375	citrus physiological premature fruit drop pectin	328:375	citrus physiological premature fruit drop pectin (CPDP)	328:382	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	4	18	theme	monosaccharide	450:463	arg1	composition					465:475	monosaccharide composition	450:475	monosaccharide composition	450:475	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	2	19	theme	acid	259:262	arg1	method					275:280	acid hydrolysis method	259:280	acid hydrolysis method	259:280	The extraction yield of pectin reached 4.4 % by acid hydrolysis method.					
36906363	1	20	theme	citrus	168:173	arg1	drop					205:208	citrus physiological premature fruit drop	168:208	citrus physiological premature fruit drop	168:208	This study is the first to extract and characterize pectin from citrus physiological premature fruit drop.					
36906363	7	21	theme	microscope	1020:1029	arg1	results					1037:1043	Scanning electron microscope (SEM) results	1002:1043	Scanning electron microscope (SEM) results	1002:1043	Scanning electron microscope (SEM) results showed CPDP had stable gel network structure.					
36906363	7	22	theme	stable	1061:1066	arg1	structure					1080:1088	stable gel network structure	1061:1088	stable gel network structure	1061:1088	Scanning electron microscope (SEM) results showed CPDP had stable gel network structure.					
36906363	4	23	theme	side	683:686	arg1	chain					688:692	galactose side chain	673:692	galactose side chain (32.02 %)	673:702	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	23	theme	side	683:686	arg1	%					701:701	32.02 %	695:701	32.02 %	695:701	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	1	24	theme	physiological	175:187	arg1	drop					205:208	citrus physiological premature fruit drop	168:208	citrus physiological premature fruit drop	168:208	This study is the first to extract and characterize pectin from citrus physiological premature fruit drop.					
36906363	0	25	theme	Structural	0:9	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics and gel properties of pectin from citrus physiological premature fruit drop.					
36906363	4	26	theme	galactose	673:681	arg1	chain					688:692	galactose side chain	673:692	galactose side chain (32.02 %)	673:702	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	26	theme	galactose	673:681	arg1	%					701:701	32.02 %	695:701	32.02 %	695:701	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	1	27	theme	premature	189:197	arg1	drop					205:208	citrus physiological premature fruit drop	168:208	citrus physiological premature fruit drop	168:208	This study is the first to extract and characterize pectin from citrus physiological premature fruit drop.					
36906363	3	28	theme	drop	365:368	arg1	CPDP					378:381	CPDP	378:381	CPDP	378:381	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	3	28	theme	drop	365:368	arg1	pectin					370:375	citrus physiological premature fruit drop pectin	328:375	citrus physiological premature fruit drop pectin (CPDP)	328:382	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	6	29	theme	storage	848:854	arg1	modulus					856:862	storage modulus	848:862	storage modulus	848:862	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	1	30	theme	fruit	199:203	arg1	drop					205:208	citrus physiological premature fruit drop	168:208	citrus physiological premature fruit drop	168:208	This study is the first to extract and characterize pectin from citrus physiological premature fruit drop.					
36906363	3	31	theme	pectin	370:375	arg1	DM					321:322	DM	321:322	DM	321:322	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	3	31	theme	pectin	370:375	arg1	methoxy-esterification					297:318	methoxy-esterification	297:318	methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP)	297:382	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	4	32	theme	branched	530:537	arg1	β					570:570	β	570:570	β	570:570	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	32	theme	branched	530:537	arg1	polysaccharide					554:567	a highly branched macromolecular polysaccharide	521:567	a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose	521:667	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	33	dep	β	570:570	arg1	0.02					573:576	0.02	573:576	0.02	573:576	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	33	dep	β	570:570	arg1	2.006 × 105 g/mol					583:599	2.006 × 105 g/mol	583:599	2.006 × 105 g/mol	583:599	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	33	dep	β	570:570	arg1	Mw					579:580	Mw	579:580	Mw	579:580	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	7	34	theme	network	1072:1078	arg1	structure					1080:1088	stable gel network structure	1061:1088	stable gel network structure	1061:1088	Scanning electron microscope (SEM) results showed CPDP had stable gel network structure.					
36906363	6	35	theme	CPDP	996:999	arg1	chains					986:991	rich neutral sugar side chains	962:991	rich neutral sugar side chains of CPDP	962:999	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	6	35	theme	CPDP	996:999	arg1	pectin					907:912	commercial citrus pectin	889:912	commercial citrus pectin (CP) used in this paper due to the lower DM	889:956	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	6	35	theme	CPDP	996:999	arg1	CP					915:916	CP	915:916	CP	915:916	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	2	36	theme	pectin	235:240	arg1	yield					226:230	The extraction yield	211:230	The extraction yield of pectin	211:240	The extraction yield of pectin reached 4.4 % by acid hydrolysis method.					
36906363	4	37	theme	I	631:631	arg1	%					647:647	50.40 %	641:647	50.40 %	641:647	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	37	theme	I	631:631	arg1	domain					633:638	rich rhamnogalacturonan I domain	607:638	rich rhamnogalacturonan I domain (50.40 %)	607:648	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	3	38	theme	low-methoxylated	415:430	arg1	pectin					432:437	low-methoxylated pectin	415:437	low-methoxylated pectin (LMP)	415:443	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	3	38	theme	low-methoxylated	415:430	arg1	LMP					440:442	LMP	440:442	LMP	440:442	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	6	39	dep	strength	835:842	arg1	the					827:829	the	827:829	the	827:829	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	0	40	theme	gel	31:33	arg1	properties					35:44	gel properties	31:44	gel properties	31:44	Structural characteristics and gel properties of pectin from citrus physiological premature fruit drop.					
36906363	7	41	theme	Scanning	1002:1009	arg1	SEM					1032:1034	SEM	1032:1034	SEM	1032:1034	Scanning electron microscope (SEM) results showed CPDP had stable gel network structure.					
36906363	7	41	theme	Scanning	1002:1009	arg1	microscope					1020:1029	Scanning electron microscope	1002:1029	Scanning electron microscope (SEM) results	1002:1043	Scanning electron microscope (SEM) results showed CPDP had stable gel network structure.					
36906363	4	42	theme	test	492:495	arg1	results					497:503	The monosaccharide composition and molar mass test results	446:503	The monosaccharide composition and molar mass test results	446:503	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	7	43	theme	gel	1068:1070	arg1	structure					1080:1088	stable gel network structure	1061:1088	stable gel network structure	1061:1088	Scanning electron microscope (SEM) results showed CPDP had stable gel network structure.					
36906363	4	44	theme	long	654:657	arg1	arabinose					659:667	long arabinose	654:667	long arabinose	654:667	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	0	45	theme	pectin	49:54	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics and gel properties of pectin from citrus physiological premature fruit drop.					
36906363	0	45	theme	pectin	49:54	arg1	properties					35:44	gel properties	31:44	gel properties	31:44	Structural characteristics and gel properties of pectin from citrus physiological premature fruit drop.					
36906363	3	46	theme	methoxy-esterification	297:318	arg1	degree					287:292	The degree	283:292	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP)	283:382	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	3	46	theme	methoxy-esterification	297:318	arg1	%					394:394	15.27 %	388:394	15.27 %	388:394	The degree of methoxy-esterification (DM) of citrus physiological premature fruit drop pectin (CPDP) was 15.27 %, indicating it was low-methoxylated pectin (LMP).					
36906363	6	47	theme	rheological	797:807	arg1	tests					809:813	Textural and rheological tests	784:813	Textural and rheological tests	784:813	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	2	48	theme	extraction	215:224	arg1	yield					226:230	The extraction yield	211:230	The extraction yield of pectin	211:240	The extraction yield of pectin reached 4.4 % by acid hydrolysis method.					
36906363	6	49	theme	sugar	975:979	arg1	chains					986:991	rich neutral sugar side chains	962:991	rich neutral sugar side chains of CPDP	962:999	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	4	50	dep	composition	465:475	arg1	The					446:448	The	446:448	The	446:448	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	1	51	from	drop	205:208	arg1	pectin					156:161	pectin	156:161	pectin from citrus physiological premature fruit drop	156:208	This study is the first to extract and characterize pectin from citrus physiological premature fruit drop.					
36906363	6	52	theme	neutral	967:973	arg1	chains					986:991	rich neutral sugar side chains	962:991	rich neutral sugar side chains of CPDP	962:999	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	7	53	dep	showed	1045:1050	arg1	had					1057:1059	had	1057:1059	showed CPDP had stable gel network structure	1045:1088	Scanning electron microscope (SEM) results showed CPDP had stable gel network structure.					
36906363	6	54	theme	rich	962:965	arg1	chains					986:991	rich neutral sugar side chains	962:991	rich neutral sugar side chains of CPDP	962:999	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	0	55	theme	physiological	68:80	arg1	drop					98:101	citrus physiological premature fruit drop	61:101	citrus physiological premature fruit drop	61:101	Structural characteristics and gel properties of pectin from citrus physiological premature fruit drop.					
36906363	0	56	from	drop	98:101	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics and gel properties of pectin from citrus physiological premature fruit drop.					
36906363	0	56	from	drop	98:101	arg1	properties					35:44	gel properties	31:44	gel properties	31:44	Structural characteristics and gel properties of pectin from citrus physiological premature fruit drop.					
36906363	4	57	with	polysaccharide	554:567	arg1	%					647:647	50.40 %	641:647	50.40 %	641:647	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	57	with	polysaccharide	554:567	arg1	arabinose					659:667	long arabinose	654:667	long arabinose	654:667	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	57	with	polysaccharide	554:567	arg1	domain					633:638	rich rhamnogalacturonan I domain	607:638	rich rhamnogalacturonan I domain (50.40 %)	607:648	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	58	theme	rich	607:610	arg1	%					647:647	50.40 %	641:647	50.40 %	641:647	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	58	theme	rich	607:610	arg1	domain					633:638	rich rhamnogalacturonan I domain	607:638	rich rhamnogalacturonan I domain (50.40 %)	607:648	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	6	59	theme	citrus	900:905	arg1	CP					915:916	CP	915:916	CP	915:916	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	6	59	theme	citrus	900:905	arg1	pectin					907:912	commercial citrus pectin	889:912	commercial citrus pectin (CP) used in this paper due to the lower DM	889:956	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	0	60	theme	citrus	61:66	arg1	drop					98:101	citrus physiological premature fruit drop	61:101	citrus physiological premature fruit drop	61:101	Structural characteristics and gel properties of pectin from citrus physiological premature fruit drop.					
36906363	6	61	theme	commercial	889:898	arg1	CP					915:916	CP	915:916	CP	915:916	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	6	61	theme	commercial	889:898	arg1	pectin					907:912	commercial citrus pectin	889:912	commercial citrus pectin (CP) used in this paper due to the lower DM	889:956	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	7	62	theme	electron	1011:1018	arg1	SEM					1032:1034	SEM	1032:1034	SEM	1032:1034	Scanning electron microscope (SEM) results showed CPDP had stable gel network structure.					
36906363	7	62	theme	electron	1011:1018	arg1	microscope					1020:1029	Scanning electron microscope	1002:1029	Scanning electron microscope (SEM) results	1002:1043	Scanning electron microscope (SEM) results showed CPDP had stable gel network structure.					
36906363	6	63	theme	gel	831:833	arg1	strength					835:842	gel strength	831:842	gel strength	831:842	Textural and rheological tests showed that the gel strength and storage modulus of CPDP were higher than commercial citrus pectin (CP) used in this paper due to the lower DM and rich neutral sugar side chains of CPDP.					
36906363	4	64	with	chain	688:692	arg1	%					647:647	50.40 %	641:647	50.40 %	641:647	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	64	with	chain	688:692	arg1	arabinose					659:667	long arabinose	654:667	long arabinose	654:667	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36906363	4	64	with	chain	688:692	arg1	domain					633:638	rich rhamnogalacturonan I domain	607:638	rich rhamnogalacturonan I domain (50.40 %)	607:648	The monosaccharide composition and molar mass test results showed CPDP was a highly branched macromolecular polysaccharide (β: 0.02, Mw: 2.006 × 105 g/mol) with rich rhamnogalacturonan I domain (50.40 %) and long arabinose and galactose side chain (32.02 %).					
36267836	5	0	theme	ascorbic	711:718	arg1	carbohydrates					672:684	carbohydrates	672:684	carbohydrates	672:684	Protein, carbohydrates, lipids, dietary fibers, ascorbic acid, tannins, anthocyanins, chlorophyll, lycopene, and β-carotene contents were assessed.					
36267836	5	0	theme	ascorbic	711:718	arg1	acid					720:723	ascorbic acid	711:723	ascorbic acid	711:723	Protein, carbohydrates, lipids, dietary fibers, ascorbic acid, tannins, anthocyanins, chlorophyll, lycopene, and β-carotene contents were assessed.					
36267836	11	1	theme	food	1436:1439	arg1	valorization					1456:1467	their food and economical valorization	1430:1467	their food and economical valorization of cashew fruits	1430:1484	These results obtained provide a scientific basis for their food and economical valorization of cashew fruits.					
36267836	11	2	theme	cashew	1472:1477	arg1	fruits					1479:1484	cashew fruits	1472:1484	cashew fruits	1472:1484	These results obtained provide a scientific basis for their food and economical valorization of cashew fruits.					
36267836	2	3	theme	compositions	324:335	arg1	comparison					282:291	a comparison	280:291	a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso	280:439	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	8	4	from	suitable	1188:1195	arg1	summary					1164:1170	summary	1164:1170	summary	1164:1170	In summary, almonds may be suitable as a source of lipids and related products.					
36267836	6	5	dep	proteins	881:888	arg1	-1.8 g/100 gDW					899:912	-1.8 g/100 gDW	899:912	-1.8 g/100 gDW	899:912	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
36267836	6	5	dep	proteins	881:888	arg1	22.32					891:895	22.32	891:895	22.32	891:895	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
36267836	0	6	theme	Apples	74:79	arg1	Comparison					0:9	Comparison	0:9	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.	0:130	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	2	7	from	areas	419:423	arg1	compositions					324:335	proximate and phytonutrient compositions	296:335	proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso	296:439	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	2	7	from	areas	419:423	arg1	apples					384:389	apples	384:389	apples from different geographical areas of Burkina Faso	384:439	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	2	7	from	areas	419:423	arg1	nuts					375:378	cashew (Anacardium occidentale L.) nuts	340:378	cashew (Anacardium occidentale L.) nuts	340:378	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	2	8	theme	phytonutrient	310:322	arg1	compositions					324:335	proximate and phytonutrient compositions	296:335	proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso	296:439	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	10	9	theme	by-product	1353:1362	arg1	processing					1364:1373	cashew by-product processing	1346:1373	cashew by-product processing	1346:1373	All of these data are important clues for cashew by-product processing.					
36267836	1	10	theme	cashew	223:228	arg1	types					190:194	two types	186:194	two types of fruits: the nut and the cashew apple	186:234	The cashew plant is an allogamous plant that produces two types of fruits: the nut and the cashew apple.					
36267836	1	10	theme	cashew	223:228	arg1	apple					230:234	the cashew apple	219:234	the cashew apple	219:234	The cashew plant is an allogamous plant that produces two types of fruits: the nut and the cashew apple.					
36267836	0	11	theme	Geographical	96:107	arg1	Areas					109:113	Different Geographical Areas	86:113	Different Geographical Areas of Burkina Faso	86:129	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	0	12	from	Comparison	0:9	arg1	Areas					109:113	Different Geographical Areas	86:113	Different Geographical Areas of Burkina Faso	86:129	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	8	13	theme	lipids	1212:1217	arg1	source					1202:1207	a source	1200:1207	a source of lipids and related products	1200:1238	In summary, almonds may be suitable as a source of lipids and related products.					
36267836	7	14	dep	pigments	1098:1105	arg1	β-carotene					1131:1140	β-carotene	1131:1140	β-carotene	1131:1140	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	14	dep	pigments	1098:1105	arg1	chlorophyll					1147:1157	chlorophyll	1147:1157	chlorophyll	1147:1157	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	14	dep	pigments	1098:1105	arg1	anthocyanin					1118:1128	anthocyanin	1118:1128	anthocyanin	1118:1128	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	14	dep	pigments	1098:1105	arg1	lycopene					1108:1115	lycopene	1108:1115	lycopene	1108:1115	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	14	dep	pigments	1098:1105	arg1	pigments					1098:1105	pigments	1098:1105	pigments (lycopene, anthocyanin, β-carotene, and chlorophyll)	1098:1158	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	15	from	pigments	1098:1105	arg1	rich					996:999	rich	996:999	rich	996:999	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	16	from	Apples	965:970	arg1	hand					986:989	the other hand	976:989	the other hand	976:989	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	0	17	theme	Different	86:94	arg1	Areas					109:113	Different Geographical Areas	86:113	Different Geographical Areas of Burkina Faso	86:129	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	11	18	theme	fruits	1479:1484	arg1	valorization					1456:1467	their food and economical valorization	1430:1467	their food and economical valorization of cashew fruits	1430:1484	These results obtained provide a scientific basis for their food and economical valorization of cashew fruits.					
36267836	5	19	theme	β-carotene	776:785	arg1	contents					787:794	β-carotene contents	776:794	β-carotene contents	776:794	Protein, carbohydrates, lipids, dietary fibers, ascorbic acid, tannins, anthocyanins, chlorophyll, lycopene, and β-carotene contents were assessed.					
36267836	6	20	from	contents	837:844	arg1	almonds					956:962	almonds	956:962	almonds	956:962	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
36267836	6	21	theme	high	832:835	arg1	contents					837:844	high contents	832:844	high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds	832:962	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
36267836	7	22	theme	other	980:984	arg1	hand					986:989	the other hand	976:989	the other hand	976:989	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	4	23	theme	cashew	583:588	arg1	nuts					590:593	cashew nuts	583:593	cashew nuts	583:593	The nutritional potential of cashew nuts and apples produced was evaluated to enhance their food processing.					
36267836	2	24	theme	present	241:247	arg1	study					249:253	The present study	237:253	The present study	237:253	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	1	25	theme	allogamous	155:164	arg1	plant					143:147	The cashew plant	132:147	The cashew plant	132:147	The cashew plant is an allogamous plant that produces two types of fruits: the nut and the cashew apple.					
36267836	1	25	theme	allogamous	155:164	arg1	plant					166:170	an allogamous plant	152:170	an allogamous plant that produces two types of fruits: the nut and the cashew apple	152:234	The cashew plant is an allogamous plant that produces two types of fruits: the nut and the cashew apple.					
36267836	5	26	theme	dietary	695:701	arg1	fibers					703:708	dietary fibers	695:708	dietary fibers	695:708	Protein, carbohydrates, lipids, dietary fibers, ascorbic acid, tannins, anthocyanins, chlorophyll, lycopene, and β-carotene contents were assessed.					
36267836	5	26	theme	dietary	695:701	arg1	carbohydrates					672:684	carbohydrates	672:684	carbohydrates	672:684	Protein, carbohydrates, lipids, dietary fibers, ascorbic acid, tannins, anthocyanins, chlorophyll, lycopene, and β-carotene contents were assessed.					
36267836	7	27	theme	ascorbic	1012:1019	arg1	acid					1021:1024	ascorbic acid	1012:1024	ascorbic acid (387.45 ± 17.4 mg/100 g)	1012:1049	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	27	theme	ascorbic	1012:1019	arg1	17.4 mg/100 g					1036:1048	387.45 ± 17.4 mg/100 g	1027:1048	387.45 ± 17.4 mg/100 g	1027:1048	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	2	28	from	compositions	324:335	arg1	areas					419:423	different geographical areas	396:423	different geographical areas of Burkina Faso	396:439	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	3	29	theme	kernels	485:491	arg1	samples					463:469	60 samples	460:469	60 samples of apples and kernels	460:491	For this purpose, 60 samples of apples and kernels were collected from the three main cashew cultivation areas.					
36267836	7	30	theme	±	1034:1034	arg1	acid					1021:1024	ascorbic acid	1012:1024	ascorbic acid (387.45 ± 17.4 mg/100 g)	1012:1049	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	30	theme	±	1034:1034	arg1	17.4 mg/100 g					1036:1048	387.45 ± 17.4 mg/100 g	1027:1048	387.45 ± 17.4 mg/100 g	1027:1048	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	10	31	theme	important	1326:1334	arg1	All					1304:1306	All	1304:1306	All	1304:1306	All of these data are important clues for cashew by-product processing.					
36267836	10	31	theme	important	1326:1334	arg1	data					1317:1320	these data	1311:1320	these data	1311:1320	All of these data are important clues for cashew by-product processing.					
36267836	10	31	theme	important	1326:1334	arg1	clues					1336:1340	important clues	1326:1340	important clues for cashew by-product processing	1326:1373	All of these data are important clues for cashew by-product processing.					
36267836	10	32	theme	cashew	1346:1351	arg1	processing					1364:1373	cashew by-product processing	1346:1373	cashew by-product processing	1346:1373	All of these data are important clues for cashew by-product processing.					
36267836	6	33	theme	±	934:934	arg1	DW					949:950	12.05 ± 1.27 g/100 g DW	928:950	12.05 ± 1.27 g/100 g DW	928:950	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
36267836	6	33	theme	±	934:934	arg1	starch					920:925	starch	920:925	starch	920:925	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
36267836	9	34	theme	natural	1263:1269	arg1	Apples					1241:1246	Apples	1241:1246	Apples	1241:1246	Apples can be used as natural antioxidants and produce juices.					
36267836	9	34	theme	natural	1263:1269	arg1	antioxidants					1271:1282	natural antioxidants	1263:1282	natural antioxidants	1263:1282	Apples can be used as natural antioxidants and produce juices.					
36267836	2	35	theme	geographical	406:417	arg1	areas					419:423	different geographical areas	396:423	different geographical areas of Burkina Faso	396:439	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	3	36	theme	main	523:526	arg1	areas					547:551	the three main cashew cultivation areas	513:551	the three main cashew cultivation areas	513:551	For this purpose, 60 samples of apples and kernels were collected from the three main cashew cultivation areas.					
36267836	9	37	used	used	1255:1258	arg2	Apples					1241:1246	Apples	1241:1246	Apples	1241:1246	Apples can be used as natural antioxidants and produce juices.					
36267836	9	37	used	used	1255:1258	arg2	antioxidants					1271:1282	natural antioxidants	1263:1282	natural antioxidants	1263:1282	Apples can be used as natural antioxidants and produce juices.					
36267836	9	37	used	used	1255:1258	arg2	juices					1296:1301	produce juices	1288:1301	produce juices	1288:1301	Apples can be used as natural antioxidants and produce juices.					
36267836	2	38	theme	different	396:404	arg1	areas					419:423	different geographical areas	396:423	different geographical areas of Burkina Faso	396:439	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	0	39	theme	Faso	126:129	arg1	Areas					109:113	Different Geographical Areas	86:113	Different Geographical Areas of Burkina Faso	86:129	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	2	40	theme	apples	384:389	arg1	compositions					324:335	proximate and phytonutrient compositions	296:335	proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso	296:439	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	0	41	theme	Proximate	14:22	arg1	Compositions					42:53	Proximate and Phytonutrient Compositions	14:53	Proximate and Phytonutrient Compositions of Cashew Nuts	14:68	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	4	42	theme	food	646:649	arg1	processing					651:660	their food processing	640:660	their food processing	640:660	The nutritional potential of cashew nuts and apples produced was evaluated to enhance their food processing.					
36267836	1	43	theme	cashew	136:141	arg1	plant					143:147	The cashew plant	132:147	The cashew plant	132:147	The cashew plant is an allogamous plant that produces two types of fruits: the nut and the cashew apple.					
36267836	1	43	theme	cashew	136:141	arg1	plant					166:170	an allogamous plant	152:170	an allogamous plant that produces two types of fruits: the nut and the cashew apple	152:234	The cashew plant is an allogamous plant that produces two types of fruits: the nut and the cashew apple.					
36267836	0	44	theme	Compositions	42:53	arg1	Comparison					0:9	Comparison	0:9	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.	0:130	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	6	45	theme	proteins	881:888	arg1	contents					837:844	high contents	832:844	high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds	832:962	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
36267836	4	46	theme	apples	599:604	arg1	potential					570:578	The nutritional potential	554:578	The nutritional potential of cashew nuts and apples produced	554:613	The nutritional potential of cashew nuts and apples produced was evaluated to enhance their food processing.					
36267836	2	47	theme	nuts	375:378	arg1	compositions					324:335	proximate and phytonutrient compositions	296:335	proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso	296:439	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	2	48	theme	Faso	436:439	arg1	areas					419:423	different geographical areas	396:423	different geographical areas of Burkina Faso	396:439	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	0	49	theme	Phytonutrient	28:40	arg1	Compositions					42:53	Proximate and Phytonutrient Compositions	14:53	Proximate and Phytonutrient Compositions of Cashew Nuts	14:68	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	1	50	theme	fruits	199:204	arg1	nut					211:213	the nut	207:213	the nut	207:213	The cashew plant is an allogamous plant that produces two types of fruits: the nut and the cashew apple.					
36267836	1	50	theme	fruits	199:204	arg1	types					190:194	two types	186:194	two types of fruits: the nut and the cashew apple	186:234	The cashew plant is an allogamous plant that produces two types of fruits: the nut and the cashew apple.					
36267836	1	50	theme	fruits	199:204	arg1	apple					230:234	the cashew apple	219:234	the cashew apple	219:234	The cashew plant is an allogamous plant that produces two types of fruits: the nut and the cashew apple.					
36267836	7	51	theme	soluble	1052:1058	arg1	sugars					1060:1065	soluble sugars	1052:1065	soluble sugars (387.45 ± 17.4 mg/100 g,)	1052:1091	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	51	theme	soluble	1052:1058	arg1	17.4 mg/100 g					1077:1089	387.45 ± 17.4 mg/100 g,	1068:1090	17.4 mg/100 g	1077:1089	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	52	from	sugars	1060:1065	arg1	rich					996:999	rich	996:999	rich	996:999	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	3	53	theme	cashew	528:533	arg1	areas					547:551	the three main cashew cultivation areas	513:551	the three main cashew cultivation areas	513:551	For this purpose, 60 samples of apples and kernels were collected from the three main cashew cultivation areas.					
36267836	7	54	from	acid	1021:1024	arg1	rich					996:999	rich	996:999	rich	996:999	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	55	from	rich	996:999	arg1	chlorophyll					1147:1157	chlorophyll	1147:1157	chlorophyll	1147:1157	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	55	from	rich	996:999	arg1	sugars					1060:1065	soluble sugars	1052:1065	soluble sugars (387.45 ± 17.4 mg/100 g,)	1052:1091	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	55	from	rich	996:999	arg1	anthocyanin					1118:1128	anthocyanin	1118:1128	anthocyanin	1118:1128	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	55	from	rich	996:999	arg1	17.4 mg/100 g					1036:1048	387.45 ± 17.4 mg/100 g	1027:1048	387.45 ± 17.4 mg/100 g	1027:1048	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	55	from	rich	996:999	arg1	β-carotene					1131:1140	β-carotene	1131:1140	β-carotene	1131:1140	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	55	from	rich	996:999	arg1	lipids					1004:1009	lipids	1004:1009	lipids	1004:1009	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	55	from	rich	996:999	arg1	pigments					1098:1105	pigments	1098:1105	pigments (lycopene, anthocyanin, β-carotene, and chlorophyll)	1098:1158	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	55	from	rich	996:999	arg1	17.4 mg/100 g					1077:1089	387.45 ± 17.4 mg/100 g,	1068:1090	17.4 mg/100 g	1077:1089	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	55	from	rich	996:999	arg1	acid					1021:1024	ascorbic acid	1012:1024	ascorbic acid (387.45 ± 17.4 mg/100 g)	1012:1049	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	55	from	rich	996:999	arg1	lycopene					1108:1115	lycopene	1108:1115	lycopene	1108:1115	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	11	56	theme	economical	1445:1454	arg1	valorization					1456:1467	their food and economical valorization	1430:1467	their food and economical valorization of cashew fruits	1430:1484	These results obtained provide a scientific basis for their food and economical valorization of cashew fruits.					
36267836	6	57	theme	lipids	849:854	arg1	contents					837:844	high contents	832:844	high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds	832:962	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
36267836	3	58	theme	cultivation	535:545	arg1	areas					547:551	the three main cashew cultivation areas	513:551	the three main cashew cultivation areas	513:551	For this purpose, 60 samples of apples and kernels were collected from the three main cashew cultivation areas.					
36267836	2	59	theme	proximate	296:304	arg1	compositions					324:335	proximate and phytonutrient compositions	296:335	proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso	296:439	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	6	60	theme	±	863:863	arg1	lipids					849:854	lipids	849:854	lipids (50.42 ± 2.3 g/100 gDW)	849:878	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
36267836	6	60	theme	±	863:863	arg1	2.3 g/100 gDW					865:877	50.42 ± 2.3 g/100 gDW	857:877	50.42 ± 2.3 g/100 gDW	857:877	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
36267836	0	61	from	Areas	109:113	arg1	Apples					74:79	Apples	74:79	Apples from Different Geographical Areas of Burkina Faso	74:129	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	0	61	from	Areas	109:113	arg1	Compositions					42:53	Proximate and Phytonutrient Compositions	14:53	Proximate and Phytonutrient Compositions of Cashew Nuts	14:68	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	0	61	from	Areas	109:113	arg1	Comparison					0:9	Comparison	0:9	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.	0:130	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	8	62	theme	related	1223:1229	arg1	products					1231:1238	related products	1223:1238	related products	1223:1238	In summary, almonds may be suitable as a source of lipids and related products.					
36267836	4	63	theme	nutritional	558:568	arg1	potential					570:578	The nutritional potential	554:578	The nutritional potential of cashew nuts and apples produced	554:613	The nutritional potential of cashew nuts and apples produced was evaluated to enhance their food processing.					
36267836	9	64	dep	juices	1296:1301	arg1	produce					1288:1294	produce	1288:1294	produce	1288:1294	Apples can be used as natural antioxidants and produce juices.					
36267836	8	65	theme	products	1231:1238	arg1	source					1202:1207	a source	1200:1207	a source of lipids and related products	1200:1238	In summary, almonds may be suitable as a source of lipids and related products.					
36267836	8	66	from	summary	1164:1170	arg1	suitable					1188:1195	suitable	1188:1195	suitable	1188:1195	In summary, almonds may be suitable as a source of lipids and related products.					
36267836	7	67	theme	±	1075:1075	arg1	sugars					1060:1065	soluble sugars	1052:1065	soluble sugars (387.45 ± 17.4 mg/100 g,)	1052:1091	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	7	67	theme	±	1075:1075	arg1	17.4 mg/100 g					1077:1089	387.45 ± 17.4 mg/100 g,	1068:1090	17.4 mg/100 g	1077:1089	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	6	68	theme	1.27 g/100 g	936:947	arg1	DW					949:950	12.05 ± 1.27 g/100 g DW	928:950	12.05 ± 1.27 g/100 g DW	928:950	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
36267836	6	68	theme	1.27 g/100 g	936:947	arg1	starch					920:925	starch	920:925	starch	920:925	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
36267836	4	69	theme	nuts	590:593	arg1	potential					570:578	The nutritional potential	554:578	The nutritional potential of cashew nuts and apples produced	554:613	The nutritional potential of cashew nuts and apples produced was evaluated to enhance their food processing.					
36267836	2	70	theme	cashew	340:345	arg1	nuts					375:378	cashew (Anacardium occidentale L.) nuts	340:378	cashew (Anacardium occidentale L.) nuts	340:378	The present study was conducted to perform a comparison of proximate and phytonutrient compositions of cashew (Anacardium occidentale L.) nuts and apples from different geographical areas of Burkina Faso.					
36267836	0	71	theme	Nuts	65:68	arg1	Apples					74:79	Apples	74:79	Apples from Different Geographical Areas of Burkina Faso	74:129	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	0	71	theme	Nuts	65:68	arg1	Compositions					42:53	Proximate and Phytonutrient Compositions	14:53	Proximate and Phytonutrient Compositions of Cashew Nuts	14:68	Comparison of Proximate and Phytonutrient Compositions of Cashew Nuts and Apples from Different Geographical Areas of Burkina Faso.					
36267836	7	72	from	lipids	1004:1009	arg1	rich					996:999	rich	996:999	rich	996:999	Apples, on the other hand, are rich in lipids, ascorbic acid (387.45 ± 17.4 mg/100 g), soluble sugars (387.45 ± 17.4 mg/100 g,), and pigments (lycopene, anthocyanin, β-carotene, and chlorophyll).					
36267836	1	73	dep	types	190:194	arg1	nut					211:213	the nut	207:213	the nut	207:213	The cashew plant is an allogamous plant that produces two types of fruits: the nut and the cashew apple.					
36267836	1	73	dep	types	190:194	arg1	types					190:194	two types	186:194	two types of fruits: the nut and the cashew apple	186:234	The cashew plant is an allogamous plant that produces two types of fruits: the nut and the cashew apple.					
36267836	1	73	dep	types	190:194	arg1	apple					230:234	the cashew apple	219:234	the cashew apple	219:234	The cashew plant is an allogamous plant that produces two types of fruits: the nut and the cashew apple.					
36267836	11	74	theme	scientific	1409:1418	arg1	basis					1420:1424	a scientific basis	1407:1424	a scientific basis for their food and economical valorization of cashew fruits	1407:1484	These results obtained provide a scientific basis for their food and economical valorization of cashew fruits.					
36267836	3	75	theme	apples	474:479	arg1	samples					463:469	60 samples	460:469	60 samples of apples and kernels	460:491	For this purpose, 60 samples of apples and kernels were collected from the three main cashew cultivation areas.					
36267836	6	76	theme	starch	920:925	arg1	contents					837:844	high contents	832:844	high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds	832:962	The results revealed high contents of lipids (50.42 ± 2.3 g/100 gDW), proteins (22.32 + -1.8 g/100 gDW), and starch (12.05 ± 1.27 g/100 g DW) in almonds.					
35714865	8	0	theme	coli	1036:1039	arg1	wall					1025:1028	the cell wall	1016:1028	the cell wall of E. coli	1016:1039	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	9	1	theme	other	1134:1138	arg1	hand					1140:1143	the other hand	1130:1143	the other hand	1130:1143	On the other hand, the release of Ag+ during cell co-culture was studied and the results showed that most of the Ag+ released was taken up by E. coli.					
35714865	2	2	theme	stable	352:357	arg1	HP-AgNPs					384:391	HP-AgNPs	384:391	HP-AgNPs	384:391	In this study, we used hesperidin and pectin as reductants and stabilizers, and prepared uniform and stable Hesperidin-Pectin AgNPs (HP-AgNPs) by a simple microwave-assisted process.					
35714865	2	2	theme	stable	352:357	arg1	AgNPs					377:381	stable Hesperidin-Pectin AgNPs	352:381	stable Hesperidin-Pectin AgNPs (HP-AgNPs)	352:392	In this study, we used hesperidin and pectin as reductants and stabilizers, and prepared uniform and stable Hesperidin-Pectin AgNPs (HP-AgNPs) by a simple microwave-assisted process.					
35714865	11	3	theme	antibacterial	1460:1472	arg1	activity					1474:1481	good antibacterial activity	1455:1481	good antibacterial activity	1455:1481	These preliminary data suggest that HP-AgNPs has good antibacterial activity and may be developed as an effective antibacterial nanomaterial.					
35714865	10	4	theme	antibacterial	1371:1383	arg1	properties					1385:1394	the antibacterial properties	1367:1394	the antibacterial properties of AgNPs	1367:1403	The synergistic effect of hesperidin and pectin resulted in a significant enhancement of the antibacterial properties of AgNPs.					
35714865	6	5	theme	66.7 μg/mL	824:833	arg1	values					814:819	the MIC values	806:819	the MIC values of 66.7 μg/mL	806:833	Antibacterial tests showed that HP-AgNPs2 showed the MIC values of 66.7 μg/mL against E. coli.					
35714865	3	6	theme	H-AgNPs	509:515	arg1	proportion					449:458	the proportion	445:458	Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs	434:515	Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs were obtained respectively.					
35714865	10	7	theme	properties	1385:1394	arg1	enhancement					1352:1362	a significant enhancement	1338:1362	a significant enhancement of the antibacterial properties of AgNPs	1338:1403	The synergistic effect of hesperidin and pectin resulted in a significant enhancement of the antibacterial properties of AgNPs.					
35714865	0	8	theme	antibacterial	100:112	arg1	mechanism					114:122	their synergistic antibacterial mechanism	82:122	their synergistic antibacterial mechanism	82:122	Biosynthesis of silver nanoparticle composites based on hesperidin and pectin and their synergistic antibacterial mechanism.					
35714865	10	9	theme	significant	1340:1350	arg1	enhancement					1352:1362	a significant enhancement	1338:1362	a significant enhancement of the antibacterial properties of AgNPs	1338:1403	The synergistic effect of hesperidin and pectin resulted in a significant enhancement of the antibacterial properties of AgNPs.					
35714865	5	10	theme	spectroscopy	674:685	arg1	analysis					694:701	spectroscopy (FTIR) analysis	674:701	spectroscopy (FTIR) analysis	674:701	Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag+ was reduced by hesperidin and pectin.					
35714865	10	11	theme	pectin	1319:1324	arg1	effect					1294:1299	The synergistic effect	1278:1299	The synergistic effect of hesperidin and pectin	1278:1324	The synergistic effect of hesperidin and pectin resulted in a significant enhancement of the antibacterial properties of AgNPs.					
35714865	1	12	used	used	166:169	arg2	AgNPs					147:151	AgNPs	147:151	AgNPs	147:151	Silver nanoparticles (AgNPs) were widely used in the antibacterial field because of their excellent antibacterial properties.					
35714865	1	12	used	used	166:169	arg2	nanoparticles					132:144	Silver nanoparticles	125:144	Silver nanoparticles (AgNPs)	125:152	Silver nanoparticles (AgNPs) were widely used in the antibacterial field because of their excellent antibacterial properties.					
35714865	4	13	theme	particle	593:600	arg1	size					602:605	the mean particle size	584:605	the mean particle size	584:605	With the increase of hesperidin ratio, the mean particle size and zeta potential increased gradually.					
35714865	0	14	theme	synergistic	88:98	arg1	mechanism					114:122	their synergistic antibacterial mechanism	82:122	their synergistic antibacterial mechanism	82:122	Biosynthesis of silver nanoparticle composites based on hesperidin and pectin and their synergistic antibacterial mechanism.					
35714865	8	15	from	damage	1006:1011	arg1	ROS					1121:1123	ROS	1121:1123	ROS	1121:1123	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	8	15	from	damage	1006:1011	arg1	species					1112:1118	reactive oxygen species	1096:1118	reactive oxygen species (ROS)	1096:1124	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	4	16	theme	mean	588:591	arg1	size					602:605	the mean particle size	584:605	the mean particle size	584:605	With the increase of hesperidin ratio, the mean particle size and zeta potential increased gradually.					
35714865	3	17	theme	P-AgNPs	475:481	arg1	proportion					449:458	the proportion	445:458	Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs	434:515	Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs were obtained respectively.					
35714865	8	18	from	increase	1084:1091	arg1	ROS					1121:1123	ROS	1121:1123	ROS	1121:1123	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	8	18	from	increase	1084:1091	arg1	species					1112:1118	reactive oxygen species	1096:1118	reactive oxygen species (ROS)	1096:1124	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	8	19	theme	cell	1020:1023	arg1	wall					1025:1028	the cell wall	1016:1028	the cell wall of E. coli	1016:1039	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	10	20	theme	AgNPs	1399:1403	arg1	properties					1385:1394	the antibacterial properties	1367:1394	the antibacterial properties of AgNPs	1367:1403	The synergistic effect of hesperidin and pectin resulted in a significant enhancement of the antibacterial properties of AgNPs.					
35714865	9	21	theme	Ag+	1161:1163	arg1	release					1150:1156	the release	1146:1156	the release of Ag+ during cell co-culture	1146:1186	On the other hand, the release of Ag+ during cell co-culture was studied and the results showed that most of the Ag+ released was taken up by E. coli.					
35714865	1	22	theme	antibacterial	178:190	arg1	field					192:196	the antibacterial field	174:196	the antibacterial field	174:196	Silver nanoparticles (AgNPs) were widely used in the antibacterial field because of their excellent antibacterial properties.					
35714865	11	23	theme	effective	1510:1518	arg1	nanomaterial					1534:1545	an effective antibacterial nanomaterial	1507:1545	an effective antibacterial nanomaterial	1507:1545	These preliminary data suggest that HP-AgNPs has good antibacterial activity and may be developed as an effective antibacterial nanomaterial.					
35714865	11	23	theme	effective	1510:1518	arg1	HP-AgNPs					1442:1449	HP-AgNPs	1442:1449	HP-AgNPs	1442:1449	These preliminary data suggest that HP-AgNPs has good antibacterial activity and may be developed as an effective antibacterial nanomaterial.					
35714865	3	24	theme	Increasing	434:443	arg1	proportion					449:458	the proportion	445:458	Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs	434:515	Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs were obtained respectively.					
35714865	0	25	theme	nanoparticle	23:34	arg1	composites					36:45	silver nanoparticle composites	16:45	silver nanoparticle composites	16:45	Biosynthesis of silver nanoparticle composites based on hesperidin and pectin and their synergistic antibacterial mechanism.					
35714865	8	26	theme	contents	1068:1075	arg1	damage					1006:1011	damage	1006:1011	damage to the cell wall of E. coli	1006:1039	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	8	26	theme	contents	1068:1075	arg1	leakage					1053:1059	leakage	1053:1059	leakage of its contents	1053:1075	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	8	26	theme	contents	1068:1075	arg1	increase					1084:1091	an increase	1081:1091	an increase in reactive oxygen species (ROS)	1081:1124	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	6	27	theme	MIC	810:812	arg1	values					814:819	the MIC values	806:819	the MIC values of 66.7 μg/mL	806:833	Antibacterial tests showed that HP-AgNPs2 showed the MIC values of 66.7 μg/mL against E. coli.					
35714865	0	28	theme	silver	16:21	arg1	composites					36:45	silver nanoparticle composites	16:45	silver nanoparticle composites	16:45	Biosynthesis of silver nanoparticle composites based on hesperidin and pectin and their synergistic antibacterial mechanism.					
35714865	4	29	theme	ratio	577:581	arg1	increase					554:561	the increase	550:561	the increase of hesperidin ratio	550:581	With the increase of hesperidin ratio, the mean particle size and zeta potential increased gradually.					
35714865	8	30	theme	Morphological	944:956	arg1	experiments					958:968	Morphological experiments	944:968	Morphological experiments	944:968	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	11	31	contain	has	1451:1453	arg2	activity					1474:1481	good antibacterial activity	1455:1481	good antibacterial activity	1455:1481	These preliminary data suggest that HP-AgNPs has good antibacterial activity and may be developed as an effective antibacterial nanomaterial.					
35714865	11	31	contain	has	1451:1453	arg1	nanomaterial					1534:1545	an effective antibacterial nanomaterial	1507:1545	an effective antibacterial nanomaterial	1507:1545	These preliminary data suggest that HP-AgNPs has good antibacterial activity and may be developed as an effective antibacterial nanomaterial.					
35714865	11	31	contain	has	1451:1453	arg1	HP-AgNPs					1442:1449	HP-AgNPs	1442:1449	HP-AgNPs	1442:1449	These preliminary data suggest that HP-AgNPs has good antibacterial activity and may be developed as an effective antibacterial nanomaterial.					
35714865	2	32	theme	microwave-assisted	406:423	arg1	process					425:431	a simple microwave-assisted process	397:431	a simple microwave-assisted process	397:431	In this study, we used hesperidin and pectin as reductants and stabilizers, and prepared uniform and stable Hesperidin-Pectin AgNPs (HP-AgNPs) by a simple microwave-assisted process.					
35714865	4	33	theme	hesperidin	566:575	arg1	ratio					577:581	hesperidin ratio	566:581	hesperidin ratio	566:581	With the increase of hesperidin ratio, the mean particle size and zeta potential increased gradually.					
35714865	2	34	theme	simple	399:404	arg1	process					425:431	a simple microwave-assisted process	397:431	a simple microwave-assisted process	397:431	In this study, we used hesperidin and pectin as reductants and stabilizers, and prepared uniform and stable Hesperidin-Pectin AgNPs (HP-AgNPs) by a simple microwave-assisted process.					
35714865	0	35	theme	composites	36:45	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis of silver nanoparticle composites	0:45	Biosynthesis of silver nanoparticle composites based on hesperidin and pectin and their synergistic antibacterial mechanism.					
35714865	5	36	dep	Fourier	647:653	arg1	transform					655:663	transform	655:663	transform infrared spectroscopy (FTIR) analysis	655:701	Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag+ was reduced by hesperidin and pectin.					
35714865	5	37	dep	transform	655:663	arg1	infrared					665:672	infrared	665:672	transform infrared spectroscopy (FTIR) analysis	655:701	Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag+ was reduced by hesperidin and pectin.					
35714865	1	38	theme	excellent	215:223	arg1	properties					239:248	their excellent antibacterial properties	209:248	their excellent antibacterial properties	209:248	Silver nanoparticles (AgNPs) were widely used in the antibacterial field because of their excellent antibacterial properties.					
35714865	8	39	from	leakage	1053:1059	arg1	ROS					1121:1123	ROS	1121:1123	ROS	1121:1123	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	8	39	from	leakage	1053:1059	arg1	species					1112:1118	reactive oxygen species	1096:1118	reactive oxygen species (ROS)	1096:1124	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	1	40	theme	Silver	125:130	arg1	AgNPs					147:151	AgNPs	147:151	AgNPs	147:151	Silver nanoparticles (AgNPs) were widely used in the antibacterial field because of their excellent antibacterial properties.					
35714865	1	40	theme	Silver	125:130	arg1	nanoparticles					132:144	Silver nanoparticles	125:144	Silver nanoparticles (AgNPs)	125:152	Silver nanoparticles (AgNPs) were widely used in the antibacterial field because of their excellent antibacterial properties.					
35714865	6	41	theme	Antibacterial	757:769	arg1	tests					771:775	Antibacterial tests	757:775	Antibacterial tests	757:775	Antibacterial tests showed that HP-AgNPs2 showed the MIC values of 66.7 μg/mL against E. coli.					
35714865	3	42	theme	HP-AgNPs2	495:503	arg1	proportion					449:458	the proportion	445:458	Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs	434:515	Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs were obtained respectively.					
35714865	9	43	theme	cell	1172:1175	arg1	co-culture					1177:1186	cell co-culture	1172:1186	cell co-culture	1172:1186	On the other hand, the release of Ag+ during cell co-culture was studied and the results showed that most of the Ag+ released was taken up by E. coli.					
35714865	8	44	theme	oxygen	1105:1110	arg1	ROS					1121:1123	ROS	1121:1123	ROS	1121:1123	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	8	44	theme	oxygen	1105:1110	arg1	species					1112:1118	reactive oxygen species	1096:1118	reactive oxygen species (ROS)	1096:1124	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	11	45	theme	antibacterial	1520:1532	arg1	nanomaterial					1534:1545	an effective antibacterial nanomaterial	1507:1545	an effective antibacterial nanomaterial	1507:1545	These preliminary data suggest that HP-AgNPs has good antibacterial activity and may be developed as an effective antibacterial nanomaterial.					
35714865	11	45	theme	antibacterial	1520:1532	arg1	HP-AgNPs					1442:1449	HP-AgNPs	1442:1449	HP-AgNPs	1442:1449	These preliminary data suggest that HP-AgNPs has good antibacterial activity and may be developed as an effective antibacterial nanomaterial.					
35714865	10	46	theme	synergistic	1282:1292	arg1	effect					1294:1299	The synergistic effect	1278:1299	The synergistic effect of hesperidin and pectin	1278:1324	The synergistic effect of hesperidin and pectin resulted in a significant enhancement of the antibacterial properties of AgNPs.					
35714865	1	47	theme	antibacterial	225:237	arg1	properties					239:248	their excellent antibacterial properties	209:248	their excellent antibacterial properties	209:248	Silver nanoparticles (AgNPs) were widely used in the antibacterial field because of their excellent antibacterial properties.					
35714865	8	48	theme	reactive	1096:1103	arg1	ROS					1121:1123	ROS	1121:1123	ROS	1121:1123	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	8	48	theme	reactive	1096:1103	arg1	species					1112:1118	reactive oxygen species	1096:1118	reactive oxygen species (ROS)	1096:1124	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	3	49	theme	HP-AgNPs1	484:492	arg1	proportion					449:458	the proportion	445:458	Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs	434:515	Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs were obtained respectively.					
35714865	11	50	theme	preliminary	1412:1422	arg1	data					1424:1427	These preliminary data	1406:1427	These preliminary data	1406:1427	These preliminary data suggest that HP-AgNPs has good antibacterial activity and may be developed as an effective antibacterial nanomaterial.					
35714865	3	51	theme	hesperidin	463:472	arg1	proportion					449:458	the proportion	445:458	Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs	434:515	Increasing the proportion of hesperidin, P-AgNPs, HP-AgNPs1, HP-AgNPs2 and H-AgNPs were obtained respectively.					
35714865	11	52	theme	good	1455:1458	arg1	activity					1474:1481	good antibacterial activity	1455:1481	good antibacterial activity	1455:1481	These preliminary data suggest that HP-AgNPs has good antibacterial activity and may be developed as an effective antibacterial nanomaterial.					
35714865	7	53	theme	antibacterial	903:915	arg1	mechanism					917:925	its antibacterial mechanism	899:925	its antibacterial mechanism against E. coli	899:941	In addition, HP-AgNPs2 was selected to clarify its antibacterial mechanism against E. coli.					
35714865	10	54	theme	hesperidin	1304:1313	arg1	effect					1294:1299	The synergistic effect	1278:1299	The synergistic effect of hesperidin and pectin	1278:1324	The synergistic effect of hesperidin and pectin resulted in a significant enhancement of the antibacterial properties of AgNPs.					
35714865	8	55	theme	HP-AgNPs2	982:990	arg1	stress					992:997	HP-AgNPs2 stress	982:997	HP-AgNPs2 stress	982:997	Morphological experiments showed that HP-AgNPs2 stress caused damage to the cell wall of E. coli, as well as leakage of its contents and an increase in reactive oxygen species (ROS).					
35714865	2	56	theme	Hesperidin-Pectin	359:375	arg1	HP-AgNPs					384:391	HP-AgNPs	384:391	HP-AgNPs	384:391	In this study, we used hesperidin and pectin as reductants and stabilizers, and prepared uniform and stable Hesperidin-Pectin AgNPs (HP-AgNPs) by a simple microwave-assisted process.					
35714865	2	56	theme	Hesperidin-Pectin	359:375	arg1	AgNPs					377:381	stable Hesperidin-Pectin AgNPs	352:381	stable Hesperidin-Pectin AgNPs (HP-AgNPs)	352:392	In this study, we used hesperidin and pectin as reductants and stabilizers, and prepared uniform and stable Hesperidin-Pectin AgNPs (HP-AgNPs) by a simple microwave-assisted process.					
35714865	2	57	used	used	269:272	arg2	we					266:267	we	266:267	we	266:267	In this study, we used hesperidin and pectin as reductants and stabilizers, and prepared uniform and stable Hesperidin-Pectin AgNPs (HP-AgNPs) by a simple microwave-assisted process.					
35714865	4	58	theme	zeta	611:614	arg1	potential					616:624	zeta potential	611:624	zeta potential	611:624	With the increase of hesperidin ratio, the mean particle size and zeta potential increased gradually.					
35472838	4	0	theme	pig	768:770	arg1	slurry					772:777	pig slurry	768:777	pig slurry	768:777	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	14	1	theme	maize	1946:1950	arg1	silage					1952:1957	maize silage	1946:1957	maize silage	1946:1957	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	6	2	theme	terrestrial	1132:1142	arg1	TAP					1159:1161	TAP	1159:1161	TAP	1159:1161	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	2	theme	terrestrial	1132:1142	arg1	categories					1020:1029	The impact categories	1009:1029	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential	1009:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	2	theme	terrestrial	1132:1142	arg1	acidification					1144:1156	terrestrial acidification	1132:1156	terrestrial acidification (TAP)	1132:1162	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	7	3	theme	lower	1316:1320	arg1	impacts					1322:1328	at least 45% lower impacts	1303:1328	at least 45% lower impacts	1303:1328	Results show that the manure scenario has at least 45% lower impacts in all assessed categories.					
35472838	7	4	theme	manure	1283:1288	arg1	scenario					1290:1297	the manure scenario	1279:1297	the manure scenario	1279:1297	Results show that the manure scenario has at least 45% lower impacts in all assessed categories.					
35472838	18	5	theme	subsequent	2419:2428	arg1	use					2430:2432	subsequent use	2419:2432	subsequent use	2419:2432	It also highlights the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry.					
35472838	2	6	theme	NFC	266:268	arg1	production					270:279	NFC production	266:279	NFC production	266:279	Nevertheless, literature assessing environmental impacts of NFC production is scarce, especially for using other starting materials than wood pulp.					
35472838	6	7	theme	global	1031:1036	arg1	GWP					1057:1059	GWP	1057:1059	GWP	1057:1059	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	7	theme	global	1031:1036	arg1	categories					1020:1029	The impact categories	1009:1029	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential	1009:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	7	theme	global	1031:1036	arg1	potential					1046:1054	global warming potential	1031:1054	global warming potential (GWP)	1031:1060	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	10	8	theme	chips	1441:1445	arg1	scenario					1447:1454	the wood chips scenario	1432:1454	the wood chips scenario	1432:1454	/kg NFC in the wood chips scenario.					
35472838	14	9	theme	waste	1866:1870	arg1	manure					1846:1851	manure	1846:1851	manure	1846:1851	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	14	9	theme	waste	1866:1870	arg1	stream					1872:1877	a waste stream	1864:1877	a waste stream	1864:1877	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	13	10	theme	main	1717:1720	arg1	difference					1722:1731	the main difference	1713:1731	the main difference	1713:1731	In the latter the main difference can be found in the substrate production.					
35472838	6	11	theme	impact	1013:1018	arg1	potential					1192:1200	terrestrial ecotoxicity potential	1168:1200	terrestrial ecotoxicity potential	1168:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	11	theme	impact	1013:1018	arg1	eutrophication					1100:1113	freshwater eutrophication	1089:1113	freshwater eutrophication	1089:1113	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	11	theme	impact	1013:1018	arg1	categories					1020:1029	The impact categories	1009:1029	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential	1009:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	11	theme	impact	1013:1018	arg1	potential					1046:1054	global warming potential	1031:1054	global warming potential (GWP)	1031:1060	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	11	theme	impact	1013:1018	arg1	scarcity					1079:1086	fossil resource scarcity	1063:1086	fossil resource scarcity	1063:1086	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	11	theme	impact	1013:1018	arg1	toxicity					1122:1129	human toxicity	1116:1129	human toxicity	1116:1129	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	11	theme	impact	1013:1018	arg1	acidification					1144:1156	terrestrial acidification	1132:1156	terrestrial acidification (TAP)	1132:1162	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	19	12	theme	Kraft	2581:2585	arg1	pulp					2587:2590	hardwood Kraft pulp	2572:2590	hardwood Kraft pulp	2572:2590	This LCA demonstrates that NFC production from manure is a sustainable alternative to the production from hardwood Kraft pulp.					
35472838	6	13	theme	freshwater	1089:1098	arg1	eutrophication					1100:1113	freshwater eutrophication	1089:1113	freshwater eutrophication	1089:1113	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	13	theme	freshwater	1089:1098	arg1	categories					1020:1029	The impact categories	1009:1029	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential	1009:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	1	14	theme	popular	163:169	arg1	cellulose					136:144	nano fibrillated cellulose	119:144	nano fibrillated cellulose (NFC)	119:150	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	1	14	theme	popular	163:169	arg1	topic					171:175	a popular topic	161:175	a popular topic of research in recent years	161:203	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	14	15	theme	upstream	1887:1894	arg1	impacts					1910:1916	no upstream environmental impacts	1884:1916	no upstream environmental impacts attached	1884:1925	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	3	16	theme	manure	511:516	arg1	production					499:508	NFC production	495:508	NFC production (manure scenario)	495:526	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	3	16	theme	manure	511:516	arg1	scenario					518:525	manure scenario	511:525	manure scenario	511:525	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	4	17	theme	chips	821:825	arg1	scenario					827:834	the wood chips scenario	812:834	the wood chips scenario	812:834	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	0	18	from	manure	81:86	arg1	assessment					25:34	Environmental life cycle assessment	0:34	Environmental life cycle assessment of nano-cellulose and biogas production from manure.	0:87	Environmental life cycle assessment of nano-cellulose and biogas production from manure.					
35472838	4	19	with	outputs	671:677	arg1	silage					757:762	maize silage	751:762	maize silage	751:762	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	4	19	with	outputs	671:677	arg1	slurry					772:777	pig slurry	768:777	pig slurry	768:777	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	6	20	theme	terrestrial	1168:1178	arg1	potential					1192:1200	terrestrial ecotoxicity potential	1168:1200	terrestrial ecotoxicity potential	1168:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	20	theme	terrestrial	1168:1178	arg1	categories					1020:1029	The impact categories	1009:1029	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential	1009:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	5	21	theme	pulp	948:951	arg1	step					960:963	the pulp to NFC step	944:963	the pulp to NFC step	944:963	A proxy approach is used to upscale the manure scenario from laboratory to an industrial scale (except for the pulp to NFC step) to ensure comparability of both scenarios.					
35472838	6	22	theme	resource	1070:1077	arg1	scarcity					1079:1086	fossil resource scarcity	1063:1086	fossil resource scarcity	1063:1086	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	22	theme	resource	1070:1077	arg1	categories					1020:1029	The impact categories	1009:1029	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential	1009:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	10	23	theme	/kg	1421:1423	arg1	NFC					1425:1427	/kg NFC	1421:1427	/kg NFC	1421:1427	/kg NFC in the wood chips scenario.					
35472838	5	24	theme	industrial	915:924	arg1	scale					926:930	an industrial scale	912:930	an industrial scale	912:930	A proxy approach is used to upscale the manure scenario from laboratory to an industrial scale (except for the pulp to NFC step) to ensure comparability of both scenarios.					
35472838	19	25	from	manure	2513:2518	arg1	alternative					2537:2547	a sustainable alternative	2523:2547	a sustainable alternative to the production from hardwood Kraft pulp	2523:2590	This LCA demonstrates that NFC production from manure is a sustainable alternative to the production from hardwood Kraft pulp.					
35472838	19	25	from	manure	2513:2518	arg1	production					2497:2506	NFC production	2493:2506	NFC production from manure	2493:2518	This LCA demonstrates that NFC production from manure is a sustainable alternative to the production from hardwood Kraft pulp.					
35472838	1	26	from	topic	171:175	arg1	years					199:203	recent years	192:203	recent years	192:203	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	7	27	contain	has	1299:1301	arg2	impacts					1322:1328	at least 45% lower impacts	1303:1328	at least 45% lower impacts	1303:1328	Results show that the manure scenario has at least 45% lower impacts in all assessed categories.					
35472838	7	27	contain	has	1299:1301	arg1	scenario					1290:1297	the manure scenario	1279:1297	the manure scenario	1279:1297	Results show that the manure scenario has at least 45% lower impacts in all assessed categories.					
35472838	18	28	dep	pulp	2441:2444	arg1	industry					2456:2463	industry	2456:2463	industry	2456:2463	It also highlights the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry.					
35472838	14	29	dep	8	1839:1839	arg1	to					1836:1837	to	1836:1837	to	1836:1837	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	2	30	theme	environmental	241:253	arg1	impacts					255:261	environmental impacts	241:261	environmental impacts of NFC production	241:279	Nevertheless, literature assessing environmental impacts of NFC production is scarce, especially for using other starting materials than wood pulp.					
35472838	17	31	theme	biogas	2282:2287	arg1	plants					2289:2294	biogas plants	2282:2294	biogas plants	2282:2294	This underlines the issue of using energy crops as substrate in biogas plants.					
35472838	18	32	from	production	2404:2413	arg1	pulp					2441:2444	pulp	2441:2444	pulp	2441:2444	It also highlights the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry.					
35472838	18	32	from	production	2404:2413	arg1	paper					2450:2454	paper	2450:2454	paper	2450:2454	It also highlights the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry.					
35472838	3	33	theme	NFC	495:497	arg1	production					499:508	NFC production	495:508	NFC production (manure scenario)	495:526	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	3	33	theme	NFC	495:497	arg1	scenario					518:525	manure scenario	511:525	manure scenario	511:525	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	15	34	theme	eq	2154:2155	arg1	eq					2154:2155	the 0.06 kg SO2 eq	2138:2155	the 0.06 kg SO2 eq	2138:2155	This difference is especially prominent in TAP, where the substrate production is responsible for 91% of the 0.06 kg SO2 eq.					
35472838	15	34	theme	eq	2154:2155	arg1	%					2133:2133	91%	2131:2133	91% of the 0.06 kg SO2 eq	2131:2155	This difference is especially prominent in TAP, where the substrate production is responsible for 91% of the 0.06 kg SO2 eq.					
35472838	1	35	theme	fibrillated	124:134	arg1	NFC					147:149	NFC	147:149	NFC	147:149	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	1	35	theme	fibrillated	124:134	arg1	topic					171:175	a popular topic	161:175	a popular topic of research in recent years	161:203	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	1	35	theme	fibrillated	124:134	arg1	cellulose					136:144	nano fibrillated cellulose	119:144	nano fibrillated cellulose (NFC)	119:150	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	13	36	located	found	1740:1744	arg1	production					1763:1772	the substrate production	1749:1772	the substrate production	1749:1772	In the latter the main difference can be found in the substrate production.					
35472838	13	36	located	found	1740:1744	arg1	latter					1706:1711	latter	1706:1711	latter	1706:1711	In the latter the main difference can be found in the substrate production.					
35472838	13	36	located	found	1740:1744	arg2	difference					1722:1731	the main difference	1713:1731	the main difference	1713:1731	In the latter the main difference can be found in the substrate production.					
35472838	18	37	theme	waste	2360:2364	arg1	inputs					2377:2382	inputs	2377:2382	inputs for the electricity production and subsequent use in the pulp and paper industry	2377:2463	It also highlights the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry.					
35472838	18	37	theme	waste	2360:2364	arg1	streams					2366:2372	waste streams	2360:2372	waste streams	2360:2372	It also highlights the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry.					
35472838	19	38	from	pulp	2587:2590	arg1	production					2556:2565	the production	2552:2565	the production from hardwood Kraft pulp	2552:2590	This LCA demonstrates that NFC production from manure is a sustainable alternative to the production from hardwood Kraft pulp.					
35472838	15	39	theme	0.06 kg	2142:2148	arg1	eq					2154:2155	the 0.06 kg SO2 eq	2138:2155	the 0.06 kg SO2 eq	2138:2155	This difference is especially prominent in TAP, where the substrate production is responsible for 91% of the 0.06 kg SO2 eq.					
35472838	14	40	theme	chips	2017:2021	arg1	scenario					2023:2030	the wood chips scenario	2008:2030	the wood chips scenario	2008:2030	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	6	41	theme	functional	1232:1241	arg1	unit					1243:1246	the functional unit	1228:1246	the functional unit of 1 kg NFC	1228:1258	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	11	42	from	pulp	1486:1489	arg1	step					1476:1479	The transformation step	1457:1479	The transformation step from pulp to NFC	1457:1496	The transformation step from pulp to NFC is identified as environmental hotspot due to the high electricity demand in both scenarios.					
35472838	11	42	from	pulp	1486:1489	arg1	hotspot					1529:1535	environmental hotspot	1515:1535	environmental hotspot due to the high electricity demand in both scenarios	1515:1588	The transformation step from pulp to NFC is identified as environmental hotspot due to the high electricity demand in both scenarios.					
35472838	5	43	theme	proxy	839:843	arg1	approach					845:852	A proxy approach	837:852	A proxy approach	837:852	A proxy approach is used to upscale the manure scenario from laboratory to an industrial scale (except for the pulp to NFC step) to ensure comparability of both scenarios.					
35472838	11	44	theme	electricity	1553:1563	arg1	demand					1565:1570	the high electricity demand	1544:1570	the high electricity demand in both scenarios	1544:1588	The transformation step from pulp to NFC is identified as environmental hotspot due to the high electricity demand in both scenarios.					
35472838	5	45	theme	NFC	956:958	arg1	step					960:963	the pulp to NFC step	944:963	the pulp to NFC step	944:963	A proxy approach is used to upscale the manure scenario from laboratory to an industrial scale (except for the pulp to NFC step) to ensure comparability of both scenarios.					
35472838	14	46	theme	subordinate	1792:1802	arg1	role					1804:1807	a subordinate role	1790:1807	a subordinate role	1790:1807	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	18	47	from	use	2430:2432	arg1	pulp					2441:2444	pulp	2441:2444	pulp	2441:2444	It also highlights the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry.					
35472838	18	47	from	use	2430:2432	arg1	paper					2450:2454	paper	2450:2454	paper	2450:2454	It also highlights the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry.					
35472838	18	48	theme	electricity	2392:2402	arg1	production					2404:2413	electricity production	2392:2413	electricity production	2392:2413	It also highlights the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry.					
35472838	12	49	theme	pulp	1681:1684	arg1	production					1686:1695	pulp production	1681:1695	pulp production	1681:1695	Results are additionally assessed only for the industrial scale part (includes biogas and pulp production).					
35472838	4	50	from	outputs	671:677	arg1	scenarios					704:712	both scenarios a typical Austrian biogas plant	699:744	both scenarios a typical Austrian biogas plant	699:744	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	19	51	theme	sustainable	2525:2535	arg1	alternative					2537:2547	a sustainable alternative	2523:2547	a sustainable alternative to the production from hardwood Kraft pulp	2523:2590	This LCA demonstrates that NFC production from manure is a sustainable alternative to the production from hardwood Kraft pulp.					
35472838	19	51	theme	sustainable	2525:2535	arg1	production					2497:2506	NFC production	2493:2506	NFC production from manure	2493:2518	This LCA demonstrates that NFC production from manure is a sustainable alternative to the production from hardwood Kraft pulp.					
35472838	8	52	theme	4.41 kg	1365:1371	arg1	eq					1377:1378	4.41 kg CO2 eq	1365:1378	4.41 kg CO2 eq	1365:1378	GWP is 4.41 kg CO2 eq.					
35472838	3	53	from	pulp	569:572	arg1	production					547:556	the production	543:556	the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA)	543:646	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	3	54	theme	new	378:380	arg1	approach					382:389	a new approach	376:389	a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario)	376:526	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	0	55	theme	Environmental	0:12	arg1	assessment					25:34	Environmental life cycle assessment	0:34	Environmental life cycle assessment of nano-cellulose and biogas production from manure.	0:87	Environmental life cycle assessment of nano-cellulose and biogas production from manure.					
35472838	14	56	from	part	2000:2003	arg1	scenario					2023:2030	the wood chips scenario	2008:2030	the wood chips scenario	2008:2030	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	14	57	theme	hotspots	1973:1980	arg1	hotspots					1973:1980	the hotspots	1969:1980	the hotspots in the industrial part in the wood chips scenario	1969:2030	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	14	57	theme	hotspots	1973:1980	arg1	production					1932:1941	the production	1928:1941	the production of maize silage	1928:1957	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	14	57	theme	hotspots	1973:1980	arg1	one					1962:1964	one	1962:1964	one	1962:1964	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	3	58	theme	manure	410:415	arg1	use					403:405	cascaded use	394:405	cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario)	394:526	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	0	59	theme	cycle	19:23	arg1	assessment					25:34	Environmental life cycle assessment	0:34	Environmental life cycle assessment of nano-cellulose and biogas production from manure.	0:87	Environmental life cycle assessment of nano-cellulose and biogas production from manure.					
35472838	1	60	theme	unique	100:105	arg1	properties					107:116	its unique properties	96:116	its unique properties	96:116	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	17	61	from	substrate	2269:2277	arg1	plants					2289:2294	biogas plants	2282:2294	biogas plants	2282:2294	This underlines the issue of using energy crops as substrate in biogas plants.					
35472838	4	62	theme	typical	716:722	arg1	plant					740:744	a typical Austrian biogas plant	714:744	both scenarios a typical Austrian biogas plant	699:744	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	18	63	theme	further	2334:2340	arg1	research					2342:2349	further research	2334:2349	further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry	2334:2463	It also highlights the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry.					
35472838	3	64	theme	use	403:405	arg1	approach					382:389	a new approach	376:389	a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario)	376:526	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	5	65	theme	scenarios	998:1006	arg1	comparability					976:988	comparability	976:988	comparability of both scenarios	976:1006	A proxy approach is used to upscale the manure scenario from laboratory to an industrial scale (except for the pulp to NFC step) to ensure comparability of both scenarios.					
35472838	9	66	theme	CO2	1414:1416	arg1	eq					1418:1419	the manure and 9.74 kg CO2 eq	1391:1419	the manure and 9.74 kg CO2 eq	1391:1419	/kg NFC in the manure and 9.74 kg CO2 eq.					
35472838	14	67	from	hotspots	1973:1980	arg1	part					2000:2003	the industrial part	1985:2003	the industrial part in the wood chips scenario	1985:2030	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	3	68	theme	hardwood	579:586	arg1	chips					588:592	hardwood chips	579:592	hardwood chips (wood chips scenario)	579:614	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	3	68	theme	hardwood	579:586	arg1	scenario					606:613	wood chips scenario	595:613	wood chips scenario	595:613	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	4	69	theme	biogas	733:738	arg1	plant					740:744	a typical Austrian biogas plant	714:744	both scenarios a typical Austrian biogas plant	699:744	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	2	70	theme	starting	319:326	arg1	materials					328:336	other starting materials	313:336	other starting materials than wood pulp	313:351	Nevertheless, literature assessing environmental impacts of NFC production is scarce, especially for using other starting materials than wood pulp.					
35472838	6	71	theme	NFC	1256:1258	arg1	unit					1243:1246	the functional unit	1228:1246	the functional unit of 1 kg NFC	1228:1258	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	16	72	theme	manure	2201:2206	arg1	scenario					2208:2215	the manure scenario	2197:2215	the manure scenario	2197:2215	impact, which is tenfold the impact of the manure scenario.					
35472838	5	73	dep	step	960:963	arg1	to					953:954	to	953:954	to	953:954	A proxy approach is used to upscale the manure scenario from laboratory to an industrial scale (except for the pulp to NFC step) to ensure comparability of both scenarios.					
35472838	1	74	theme	recent	192:197	arg1	years					199:203	recent years	192:203	recent years	192:203	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	12	75	theme	scale	1649:1653	arg1	part					1655:1658	the industrial scale part	1634:1658	the industrial scale part (includes biogas and pulp production)	1634:1696	Results are additionally assessed only for the industrial scale part (includes biogas and pulp production).					
35472838	12	76	dep	part	1655:1658	arg1	includes					1661:1668	includes	1661:1668	includes biogas and pulp production	1661:1695	Results are additionally assessed only for the industrial scale part (includes biogas and pulp production).					
35472838	3	77	theme	chips	600:604	arg1	chips					588:592	hardwood chips	579:592	hardwood chips (wood chips scenario)	579:614	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	3	77	theme	chips	600:604	arg1	scenario					606:613	wood chips scenario	595:613	wood chips scenario	595:613	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	14	78	theme	silage	1952:1957	arg1	production					1932:1941	the production	1928:1941	the production of maize silage	1928:1957	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	14	78	theme	silage	1952:1957	arg1	hotspots					1973:1980	the hotspots	1969:1980	the hotspots in the industrial part in the wood chips scenario	1969:2030	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	14	78	theme	silage	1952:1957	arg1	one					1962:1964	one	1962:1964	one	1962:1964	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	10	79	dep	NFC	1425:1427	arg1	scenario					1447:1454	the wood chips scenario	1432:1454	the wood chips scenario	1432:1454	/kg NFC in the wood chips scenario.					
35472838	3	80	theme	life	620:623	arg1	LCA					643:645	LCA	643:645	LCA	643:645	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	3	80	theme	life	620:623	arg1	assessment					631:640	life cycle assessment	620:640	life cycle assessment (LCA)	620:646	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	2	81	theme	production	270:279	arg1	impacts					255:261	environmental impacts	241:261	environmental impacts of NFC production	241:279	Nevertheless, literature assessing environmental impacts of NFC production is scarce, especially for using other starting materials than wood pulp.					
35472838	18	82	dep	production	2404:2413	arg1	the					2388:2390	the	2388:2390	the	2388:2390	It also highlights the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry.					
35472838	6	83	theme	warming	1038:1044	arg1	GWP					1057:1059	GWP	1057:1059	GWP	1057:1059	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	83	theme	warming	1038:1044	arg1	categories					1020:1029	The impact categories	1009:1029	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential	1009:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	83	theme	warming	1038:1044	arg1	potential					1046:1054	global warming potential	1031:1054	global warming potential (GWP)	1031:1060	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	3	84	from	chips	588:592	arg1	pulp					569:572	Kraft pulp	563:572	Kraft pulp from hardwood chips (wood chips scenario)	563:614	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	6	85	theme	human	1116:1120	arg1	categories					1020:1029	The impact categories	1009:1029	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential	1009:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	85	theme	human	1116:1120	arg1	toxicity					1122:1129	human toxicity	1116:1129	human toxicity	1116:1129	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	13	86	theme	substrate	1753:1761	arg1	production					1763:1772	the substrate production	1749:1772	the substrate production	1749:1772	In the latter the main difference can be found in the substrate production.					
35472838	4	87	dep	scenarios	704:712	arg1	plant					740:744	a typical Austrian biogas plant	714:744	both scenarios a typical Austrian biogas plant	699:744	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	19	88	theme	hardwood	2572:2579	arg1	pulp					2587:2590	hardwood Kraft pulp	2572:2590	hardwood Kraft pulp	2572:2590	This LCA demonstrates that NFC production from manure is a sustainable alternative to the production from hardwood Kraft pulp.					
35472838	11	89	theme	transformation	1461:1474	arg1	step					1476:1479	The transformation step	1457:1479	The transformation step from pulp to NFC	1457:1496	The transformation step from pulp to NFC is identified as environmental hotspot due to the high electricity demand in both scenarios.					
35472838	11	89	theme	transformation	1461:1474	arg1	hotspot					1529:1535	environmental hotspot	1515:1535	environmental hotspot due to the high electricity demand in both scenarios	1515:1588	The transformation step from pulp to NFC is identified as environmental hotspot due to the high electricity demand in both scenarios.					
35472838	4	90	theme	maize	751:755	arg1	silage					757:762	maize silage	751:762	maize silage	751:762	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	14	91	theme	environmental	1896:1908	arg1	impacts					1910:1916	no upstream environmental impacts	1884:1916	no upstream environmental impacts attached	1884:1925	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	3	92	dep	production	547:556	arg1	is					528:529	is	528:529	is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA)	528:646	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	11	93	from	demand	1565:1570	arg1	scenarios					1580:1588	both scenarios	1575:1588	both scenarios	1575:1588	The transformation step from pulp to NFC is identified as environmental hotspot due to the high electricity demand in both scenarios.					
35472838	6	94	dep	categories	1020:1029	arg1	potential					1192:1200	terrestrial ecotoxicity potential	1168:1200	terrestrial ecotoxicity potential	1168:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	94	dep	categories	1020:1029	arg1	eutrophication					1100:1113	freshwater eutrophication	1089:1113	freshwater eutrophication	1089:1113	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	94	dep	categories	1020:1029	arg1	categories					1020:1029	The impact categories	1009:1029	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential	1009:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	94	dep	categories	1020:1029	arg1	TAP					1159:1161	TAP	1159:1161	TAP	1159:1161	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	94	dep	categories	1020:1029	arg1	potential					1046:1054	global warming potential	1031:1054	global warming potential (GWP)	1031:1060	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	94	dep	categories	1020:1029	arg1	scarcity					1079:1086	fossil resource scarcity	1063:1086	fossil resource scarcity	1063:1086	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	94	dep	categories	1020:1029	arg1	toxicity					1122:1129	human toxicity	1116:1129	human toxicity	1116:1129	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	94	dep	categories	1020:1029	arg1	GWP					1057:1059	GWP	1057:1059	GWP	1057:1059	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	94	dep	categories	1020:1029	arg1	acidification					1144:1156	terrestrial acidification	1132:1156	terrestrial acidification (TAP)	1132:1162	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	95	theme	ecotoxicity	1180:1190	arg1	potential					1192:1200	terrestrial ecotoxicity potential	1168:1200	terrestrial ecotoxicity potential	1168:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	95	theme	ecotoxicity	1180:1190	arg1	categories					1020:1029	The impact categories	1009:1029	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential	1009:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	15	96	theme	substrate	2091:2099	arg1	responsible					2115:2125	responsible	2115:2125	responsible	2115:2125	This difference is especially prominent in TAP, where the substrate production is responsible for 91% of the 0.06 kg SO2 eq.					
35472838	15	96	theme	substrate	2091:2099	arg1	production					2101:2110	the substrate production	2087:2110	the substrate production	2087:2110	This difference is especially prominent in TAP, where the substrate production is responsible for 91% of the 0.06 kg SO2 eq.					
35472838	5	97	from	laboratory	898:907	arg1	scenario					884:891	the manure scenario	873:891	the manure scenario from laboratory to an industrial scale	873:930	A proxy approach is used to upscale the manure scenario from laboratory to an industrial scale (except for the pulp to NFC step) to ensure comparability of both scenarios.					
35472838	1	98	theme	research	180:187	arg1	cellulose					136:144	nano fibrillated cellulose	119:144	nano fibrillated cellulose (NFC)	119:150	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	1	98	theme	research	180:187	arg1	topic					171:175	a popular topic	161:175	a popular topic of research in recent years	161:203	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	4	99	theme	wood	816:819	arg1	scenario					827:834	the wood chips scenario	812:834	the wood chips scenario	812:834	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	4	100	dep	outputs	671:677	arg1	NFC					680:682	NFC	680:682	NFC	680:682	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	4	100	dep	outputs	671:677	arg1	outputs					671:677	comparable outputs	660:677	comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry	660:777	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	4	100	dep	outputs	671:677	arg1	biogas					688:693	biogas	688:693	biogas	688:693	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	4	101	theme	input	782:786	arg1	material					788:795	input material	782:795	input material	782:795	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	6	102	theme	fossil	1063:1068	arg1	scarcity					1079:1086	fossil resource scarcity	1063:1086	fossil resource scarcity	1063:1086	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	6	102	theme	fossil	1063:1068	arg1	categories					1020:1029	The impact categories	1009:1029	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential	1009:1200	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	16	103	dep	impact	2187:2192	arg1	impact					2158:2163	impact	2158:2163	impact	2158:2163	impact, which is tenfold the impact of the manure scenario.					
35472838	3	104	theme	Kraft	563:567	arg1	pulp					569:572	Kraft pulp	563:572	Kraft pulp from hardwood chips (wood chips scenario)	563:614	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	1	105	theme	nano	119:122	arg1	NFC					147:149	NFC	147:149	NFC	147:149	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	1	105	theme	nano	119:122	arg1	topic					171:175	a popular topic	161:175	a popular topic of research in recent years	161:203	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	1	105	theme	nano	119:122	arg1	cellulose					136:144	nano fibrillated cellulose	119:144	nano fibrillated cellulose (NFC)	119:150	Due to its unique properties, nano fibrillated cellulose (NFC) has been a popular topic of research in recent years.					
35472838	11	106	theme	high	1548:1551	arg1	demand					1565:1570	the high electricity demand	1544:1570	the high electricity demand in both scenarios	1544:1588	The transformation step from pulp to NFC is identified as environmental hotspot due to the high electricity demand in both scenarios.					
35472838	6	107	theme	1 kg	1251:1254	arg1	NFC					1256:1258	1 kg NFC	1251:1258	1 kg NFC	1251:1258	The impact categories global warming potential (GWP), fossil resource scarcity, freshwater eutrophication, human toxicity, terrestrial acidification (TAP) and terrestrial ecotoxicity potential are analysed referring to the functional unit of 1 kg NFC.					
35472838	19	108	theme	NFC	2493:2495	arg1	alternative					2537:2547	a sustainable alternative	2523:2547	a sustainable alternative to the production from hardwood Kraft pulp	2523:2590	This LCA demonstrates that NFC production from manure is a sustainable alternative to the production from hardwood Kraft pulp.					
35472838	19	108	theme	NFC	2493:2495	arg1	production					2497:2506	NFC production	2493:2506	NFC production from manure	2493:2518	This LCA demonstrates that NFC production from manure is a sustainable alternative to the production from hardwood Kraft pulp.					
35472838	15	109	from	prominent	2063:2071	arg1	TAP					2076:2078	TAP	2076:2078	TAP	2076:2078	This difference is especially prominent in TAP, where the substrate production is responsible for 91% of the 0.06 kg SO2 eq.					
35472838	15	110	theme	SO2	2150:2152	arg1	eq					2154:2155	the 0.06 kg SO2 eq	2138:2155	the 0.06 kg SO2 eq	2138:2155	This difference is especially prominent in TAP, where the substrate production is responsible for 91% of the 0.06 kg SO2 eq.					
35472838	9	111	from	NFC	1384:1386	arg1	eq					1418:1419	the manure and 9.74 kg CO2 eq	1391:1419	the manure and 9.74 kg CO2 eq	1391:1419	/kg NFC in the manure and 9.74 kg CO2 eq.					
35472838	15	112	from	TAP	2076:2078	arg1	prominent					2063:2071	prominent	2063:2071	prominent	2063:2071	This difference is especially prominent in TAP, where the substrate production is responsible for 91% of the 0.06 kg SO2 eq.					
35472838	3	113	contain	containing	470:479	arg2	digestate					481:489	digestate	481:489	digestate for NFC production (manure scenario)	481:526	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	3	113	contain	containing	470:479	arg1	cellulose					460:468	the cellulose	456:468	the cellulose containing digestate for NFC production (manure scenario)	456:526	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	14	114	theme	wood	2012:2015	arg1	scenario					2023:2030	the wood chips scenario	2008:2030	the wood chips scenario	2008:2030	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	3	115	from	production	547:556	arg1	study					369:373	this study	364:373	this study	364:373	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	9	116	theme	/kg	1380:1382	arg1	NFC					1384:1386	/kg NFC	1380:1386	/kg NFC in the manure and 9.74 kg CO2 eq.	1380:1420	/kg NFC in the manure and 9.74 kg CO2 eq.					
35472838	8	117	theme	CO2	1373:1375	arg1	eq					1377:1378	4.41 kg CO2 eq	1365:1378	4.41 kg CO2 eq	1365:1378	GWP is 4.41 kg CO2 eq.					
35472838	14	118	theme	industrial	1989:1998	arg1	part					2000:2003	the industrial part	1985:2003	the industrial part in the wood chips scenario	1985:2030	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	3	119	theme	cascaded	394:401	arg1	use					403:405	cascaded use	394:405	cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario)	394:526	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	0	120	theme	life	14:17	arg1	assessment					25:34	Environmental life cycle assessment	0:34	Environmental life cycle assessment of nano-cellulose and biogas production from manure.	0:87	Environmental life cycle assessment of nano-cellulose and biogas production from manure.					
35472838	16	121	theme	scenario	2208:2215	arg1	impact					2187:2192	the impact	2183:2192	the impact of the manure scenario	2183:2215	impact, which is tenfold the impact of the manure scenario.					
35472838	17	122	theme	energy	2253:2258	arg1	crops					2260:2264	energy crops	2253:2264	energy crops	2253:2264	This underlines the issue of using energy crops as substrate in biogas plants.					
35472838	10	123	theme	wood	1436:1439	arg1	scenario					1447:1454	the wood chips scenario	1432:1454	the wood chips scenario	1432:1454	/kg NFC in the wood chips scenario.					
35472838	9	124	theme	manure	1395:1400	arg1	eq					1418:1419	the manure and 9.74 kg CO2 eq	1391:1419	the manure and 9.74 kg CO2 eq	1391:1419	/kg NFC in the manure and 9.74 kg CO2 eq.					
35472838	4	125	theme	comparable	660:669	arg1	NFC					680:682	NFC	680:682	NFC	680:682	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	4	125	theme	comparable	660:669	arg1	outputs					671:677	comparable outputs	660:677	comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry	660:777	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	4	125	theme	comparable	660:669	arg1	biogas					688:693	biogas	688:693	biogas	688:693	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	18	126	theme	research	2342:2349	arg1	importance					2320:2329	the importance	2316:2329	the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry	2316:2463	It also highlights the importance of further research of using waste streams as inputs for the electricity production and subsequent use in the pulp and paper industry.					
35472838	5	127	used	used	857:860	arg2	approach					845:852	A proxy approach	837:852	A proxy approach	837:852	A proxy approach is used to upscale the manure scenario from laboratory to an industrial scale (except for the pulp to NFC step) to ensure comparability of both scenarios.					
35472838	0	128	theme	nano-cellulose	39:52	arg1	assessment					25:34	Environmental life cycle assessment	0:34	Environmental life cycle assessment of nano-cellulose and biogas production from manure.	0:87	Environmental life cycle assessment of nano-cellulose and biogas production from manure.					
35472838	9	129	theme	9.74 kg	1406:1412	arg1	eq					1418:1419	the manure and 9.74 kg CO2 eq	1391:1419	the manure and 9.74 kg CO2 eq	1391:1419	/kg NFC in the manure and 9.74 kg CO2 eq.					
35472838	0	130	theme	biogas	58:63	arg1	assessment					25:34	Environmental life cycle assessment	0:34	Environmental life cycle assessment of nano-cellulose and biogas production from manure.	0:87	Environmental life cycle assessment of nano-cellulose and biogas production from manure.					
35472838	12	131	theme	industrial	1638:1647	arg1	part					1655:1658	the industrial scale part	1634:1658	the industrial scale part (includes biogas and pulp production)	1634:1696	Results are additionally assessed only for the industrial scale part (includes biogas and pulp production).					
35472838	0	132	dep	nano-cellulose	39:52	arg1	production					65:74	production	65:74	production	65:74	Environmental life cycle assessment of nano-cellulose and biogas production from manure.					
35472838	14	133	theme	manure	1816:1821	arg1	%					1840:1840	up to 8%	1833:1840	up to 8%	1833:1840	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	14	133	theme	manure	1816:1821	arg1	scenario					1823:1830	the manure scenario	1812:1830	the manure scenario (up to 8%)	1812:1841	While it plays a subordinate role in the manure scenario (up to 8%) as manure is seen as a waste stream with no upstream environmental impacts attached, the production of maize silage is one of the hotspots in the industrial part in the wood chips scenario.					
35472838	11	134	theme	environmental	1515:1527	arg1	step					1476:1479	The transformation step	1457:1479	The transformation step from pulp to NFC	1457:1496	The transformation step from pulp to NFC is identified as environmental hotspot due to the high electricity demand in both scenarios.					
35472838	11	134	theme	environmental	1515:1527	arg1	hotspot					1529:1535	environmental hotspot	1515:1535	environmental hotspot due to the high electricity demand in both scenarios	1515:1588	The transformation step from pulp to NFC is identified as environmental hotspot due to the high electricity demand in both scenarios.					
35472838	4	135	theme	Austrian	724:731	arg1	plant					740:744	a typical Austrian biogas plant	714:744	both scenarios a typical Austrian biogas plant	699:744	To produce comparable outputs (NFC and biogas) in both scenarios a typical Austrian biogas plant with maize silage and pig slurry as input material is included in the wood chips scenario.					
35472838	2	136	theme	other	313:317	arg1	materials					328:336	other starting materials	313:336	other starting materials than wood pulp	313:351	Nevertheless, literature assessing environmental impacts of NFC production is scarce, especially for using other starting materials than wood pulp.					
35472838	3	137	theme	wood	595:598	arg1	chips					588:592	hardwood chips	579:592	hardwood chips (wood chips scenario)	579:614	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	3	137	theme	wood	595:598	arg1	scenario					606:613	wood chips scenario	595:613	wood chips scenario	595:613	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	7	138	theme	assessed	1337:1344	arg1	categories					1346:1355	all assessed categories	1333:1355	all assessed categories	1333:1355	Results show that the manure scenario has at least 45% lower impacts in all assessed categories.					
35472838	5	139	theme	manure	877:882	arg1	scenario					884:891	the manure scenario	873:891	the manure scenario from laboratory to an industrial scale	873:930	A proxy approach is used to upscale the manure scenario from laboratory to an industrial scale (except for the pulp to NFC step) to ensure comparability of both scenarios.					
35472838	3	140	theme	cycle	625:629	arg1	LCA					643:645	LCA	643:645	LCA	643:645	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	3	140	theme	cycle	625:629	arg1	assessment					631:640	life cycle assessment	620:640	life cycle assessment (LCA)	620:646	Hence, in this study, a new approach of cascaded use of manure to produce biogas and subsequently use the cellulose containing digestate for NFC production (manure scenario) is compared to the production from Kraft pulp from hardwood chips (wood chips scenario) via life cycle assessment (LCA).					
35472838	2	141	theme	wood	343:346	arg1	pulp					348:351	wood pulp	343:351	wood pulp	343:351	Nevertheless, literature assessing environmental impacts of NFC production is scarce, especially for using other starting materials than wood pulp.					
35980327	6	0	theme	intestinal	939:948	arg1	microflora					950:959	intestinal microflora	939:959	intestinal microflora	939:959	The 16s rDNA sequencing revealed that BBH was able to modulate the composition of intestinal microflora, especially the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas.					
35980327	7	1	theme	therapeutic	1147:1157	arg1	effects					1159:1165	the therapeutic effects	1143:1165	the therapeutic effects of BBH	1143:1172	The results of PICRUSt indicated that the therapeutic effects of BBH were tightly connected with DNA synthesis, replication and repair of gut microbiota.					
35980327	0	2	theme	Sodium-Induced	82:95	arg1	Colitis					97:103	Dextran Sulfate Sodium-Induced Colitis	66:103	Dextran Sulfate Sodium-Induced Colitis	66:103	A Combination of Baicalin and Berberine Hydrochloride Ameliorates Dextran Sulfate Sodium-Induced Colitis by Modulating Colon Gut Microbiota.					
35980327	4	3	theme	pharmacological	524:538	arg1	evaluations					540:550	pharmacological evaluations	524:550	pharmacological evaluations	524:550	The results of pharmacological evaluations demonstrated the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS).					
35980327	2	4	theme	compounded	364:373	arg1	formulations					375:386	compounded formulations	364:386	compounded formulations	364:386	S. Radix and C. Rhizoma are two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis.					
35980327	2	5	theme	traditional	303:313	arg1	herbs					323:327	two traditional Chinese herbs	299:327	two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis	299:403	S. Radix and C. Rhizoma are two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis.					
35980327	2	5	theme	traditional	303:313	arg1	Rhizoma					287:293	Rhizoma	287:293	Rhizoma	287:293	S. Radix and C. Rhizoma are two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis.					
35980327	2	5	theme	traditional	303:313	arg1	Radix					274:278	Radix	274:278	Radix	274:278	S. Radix and C. Rhizoma are two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis.					
35980327	5	6	theme	morphological	679:691	arg1	condition					693:701	the morphological condition	675:701	the morphological condition of colitis in mice	675:720	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	0	7	theme	Gut	125:127	arg1	Microbiota					129:138	Colon Gut Microbiota	119:138	Colon Gut Microbiota	119:138	A Combination of Baicalin and Berberine Hydrochloride Ameliorates Dextran Sulfate Sodium-Induced Colitis by Modulating Colon Gut Microbiota.					
35980327	7	8	theme	PICRUSt	1120:1126	arg1	results					1109:1115	The results	1105:1115	The results of PICRUSt	1105:1126	The results of PICRUSt indicated that the therapeutic effects of BBH were tightly connected with DNA synthesis, replication and repair of gut microbiota.					
35980327	6	9	theme	Christensenellaceae_R-7_group	1058:1086	arg1	abundances					977:986	the abundances	973:986	the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas	973:1102	The 16s rDNA sequencing revealed that BBH was able to modulate the composition of intestinal microflora, especially the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas.					
35980327	1	10	theme	main	186:189	arg1	compositions					200:211	the main chemical compositions	182:211	the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma	182:254	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	1	10	theme	main	186:189	arg1	Baicalin					141:148	Baicalin	141:148	Baicalin	141:148	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	1	10	theme	main	186:189	arg1	hydrochloride					164:176	berberine hydrochloride	154:176	berberine hydrochloride	154:176	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	0	11	theme	Colon	119:123	arg1	Microbiota					129:138	Colon Gut Microbiota	119:138	Colon Gut Microbiota	119:138	A Combination of Baicalin and Berberine Hydrochloride Ameliorates Dextran Sulfate Sodium-Induced Colitis by Modulating Colon Gut Microbiota.					
35980327	6	12	theme	rDNA	865:868	arg1	sequencing					870:879	The 16s rDNA sequencing	857:879	The 16s rDNA sequencing	857:879	The 16s rDNA sequencing revealed that BBH was able to modulate the composition of intestinal microflora, especially the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas.					
35980327	6	13	theme	Holdemania	1017:1026	arg1	abundances					977:986	the abundances	973:986	the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas	973:1102	The 16s rDNA sequencing revealed that BBH was able to modulate the composition of intestinal microflora, especially the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas.					
35980327	1	14	theme	chemical	191:198	arg1	compositions					200:211	the main chemical compositions	182:211	the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma	182:254	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	1	14	theme	chemical	191:198	arg1	Baicalin					141:148	Baicalin	141:148	Baicalin	141:148	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	1	14	theme	chemical	191:198	arg1	hydrochloride					164:176	berberine hydrochloride	154:176	berberine hydrochloride	154:176	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	7	15	theme	DNA	1202:1204	arg1	synthesis					1206:1214	DNA synthesis	1202:1214	DNA synthesis	1202:1214	The results of PICRUSt indicated that the therapeutic effects of BBH were tightly connected with DNA synthesis, replication and repair of gut microbiota.					
35980327	6	16	theme	16s	861:863	arg1	sequencing					870:879	The 16s rDNA sequencing	857:879	The 16s rDNA sequencing	857:879	The 16s rDNA sequencing revealed that BBH was able to modulate the composition of intestinal microflora, especially the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas.					
35980327	7	17	theme	gut	1243:1245	arg1	microbiota					1247:1256	gut microbiota	1243:1256	gut microbiota	1243:1256	The results of PICRUSt indicated that the therapeutic effects of BBH were tightly connected with DNA synthesis, replication and repair of gut microbiota.					
35980327	2	18	used	used	347:350	arg2	Rhizoma					287:293	Rhizoma	287:293	Rhizoma	287:293	S. Radix and C. Rhizoma are two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis.					
35980327	2	18	used	used	347:350	arg2	herbs					323:327	two traditional Chinese herbs	299:327	two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis	299:403	S. Radix and C. Rhizoma are two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis.					
35980327	2	18	used	used	347:350	arg2	Radix					274:278	Radix	274:278	Radix	274:278	S. Radix and C. Rhizoma are two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis.					
35980327	6	19	theme	Sellimonas	1093:1102	arg1	abundances					977:986	the abundances	973:986	the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas	973:1102	The 16s rDNA sequencing revealed that BBH was able to modulate the composition of intestinal microflora, especially the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas.					
35980327	3	20	theme	berberine	449:457	arg1	hydrochloride					459:471	berberine hydrochloride	449:471	berberine hydrochloride (BBH)	449:477	Therefore, the combination of Baicalin and berberine hydrochloride (BBH) to treat colitis was studied.					
35980327	3	20	theme	berberine	449:457	arg1	BBH					474:476	BBH	474:476	BBH	474:476	Therefore, the combination of Baicalin and berberine hydrochloride (BBH) to treat colitis was studied.					
35980327	0	21	theme	Baicalin	17:24	arg1	Combination					2:12	A Combination	0:12	A Combination of Baicalin and Berberine Hydrochloride	0:52	A Combination of Baicalin and Berberine Hydrochloride Ameliorates Dextran Sulfate Sodium-Induced Colitis by Modulating Colon Gut Microbiota.					
35980327	4	22	theme	protective	579:588	arg1	effects					590:596	the excellent protective effects	565:596	the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS)	565:654	The results of pharmacological evaluations demonstrated the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS).					
35980327	8	23	theme	gut	1401:1403	arg1	microbiota					1405:1414	gut microbiota	1401:1414	gut microbiota	1401:1414	In summary, it was concluded that BBH could protect mice against DSS-induced colitis, and the protective effects were tightly correlated with gut microbiota.					
35980327	5	24	theme	cytokines	829:837	arg1	balance					739:745	the balance	735:745	the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10)	735:854	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	25	theme	anti-inflammation	811:827	arg1	IL-4					840:843	IL-4	840:843	IL-4	840:843	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	25	theme	anti-inflammation	811:827	arg1	cytokines					829:837	anti-inflammation cytokines	811:837	anti-inflammation cytokines (IL-4 and IL-10)	811:854	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	25	theme	anti-inflammation	811:827	arg1	IL-10					849:853	IL-10	849:853	IL-10	849:853	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	4	26	theme	BBH	601:603	arg1	effects					590:596	the excellent protective effects	565:596	the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS)	565:654	The results of pharmacological evaluations demonstrated the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS).					
35980327	2	27	theme	Chinese	315:321	arg1	herbs					323:327	two traditional Chinese herbs	299:327	two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis	299:403	S. Radix and C. Rhizoma are two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis.					
35980327	2	27	theme	Chinese	315:321	arg1	Rhizoma					287:293	Rhizoma	287:293	Rhizoma	287:293	S. Radix and C. Rhizoma are two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis.					
35980327	2	27	theme	Chinese	315:321	arg1	Radix					274:278	Radix	274:278	Radix	274:278	S. Radix and C. Rhizoma are two traditional Chinese herbs that are commonly used together in compounded formulations to treat colitis.					
35980327	1	28	theme	Radix	229:233	arg1	compositions					200:211	the main chemical compositions	182:211	the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma	182:254	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	1	28	theme	Radix	229:233	arg1	Baicalin					141:148	Baicalin	141:148	Baicalin	141:148	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	1	28	theme	Radix	229:233	arg1	hydrochloride					164:176	berberine hydrochloride	154:176	berberine hydrochloride	154:176	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	0	29	theme	Berberine	30:38	arg1	Hydrochloride					40:52	Berberine Hydrochloride	30:52	Berberine Hydrochloride	30:52	A Combination of Baicalin and Berberine Hydrochloride Ameliorates Dextran Sulfate Sodium-Induced Colitis by Modulating Colon Gut Microbiota.					
35980327	3	30	theme	hydrochloride	459:471	arg1	combination					421:431	the combination	417:431	the combination of Baicalin and berberine hydrochloride (BBH) to treat colitis	417:494	Therefore, the combination of Baicalin and berberine hydrochloride (BBH) to treat colitis was studied.					
35980327	3	31	theme	Baicalin	436:443	arg1	combination					421:431	the combination	417:431	the combination of Baicalin and berberine hydrochloride (BBH) to treat colitis	417:494	Therefore, the combination of Baicalin and berberine hydrochloride (BBH) to treat colitis was studied.					
35980327	4	32	from	effects	590:596	arg1	colitis					608:614	colitis	608:614	colitis induced by dextran sulfate sodium (DSS)	608:654	The results of pharmacological evaluations demonstrated the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS).					
35980327	5	33	theme	cytokines	766:774	arg1	balance					739:745	the balance	735:745	the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10)	735:854	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	4	34	theme	evaluations	540:550	arg1	results					513:519	The results	509:519	The results of pharmacological evaluations	509:550	The results of pharmacological evaluations demonstrated the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS).					
35980327	6	35	theme	microflora	950:959	arg1	composition					924:934	the composition	920:934	the composition of intestinal microflora	920:959	The 16s rDNA sequencing revealed that BBH was able to modulate the composition of intestinal microflora, especially the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas.					
35980327	0	36	theme	Hydrochloride	40:52	arg1	Combination					2:12	A Combination	0:12	A Combination of Baicalin and Berberine Hydrochloride	0:52	A Combination of Baicalin and Berberine Hydrochloride Ameliorates Dextran Sulfate Sodium-Induced Colitis by Modulating Colon Gut Microbiota.					
35980327	4	37	theme	excellent	569:577	arg1	effects					590:596	the excellent protective effects	565:596	the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS)	565:654	The results of pharmacological evaluations demonstrated the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS).					
35980327	5	38	dep	cytokines	766:774	arg1	IL-6					777:780	IL-6	777:780	IL-6	777:780	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	38	dep	cytokines	766:774	arg1	cytokines					766:774	proinflammation cytokines	750:774	proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α)	750:805	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	38	dep	cytokines	766:774	arg1	IL-1β					789:793	IL-1β	789:793	IL-1β	789:793	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	38	dep	cytokines	766:774	arg1	TNF-α					800:804	TNF-α	800:804	TNF-α	800:804	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	38	dep	cytokines	766:774	arg1	IL-8					783:786	IL-8	783:786	IL-8	783:786	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	7	39	theme	microbiota	1247:1256	arg1	repair					1233:1238	repair	1233:1238	repair	1233:1238	The results of PICRUSt indicated that the therapeutic effects of BBH were tightly connected with DNA synthesis, replication and repair of gut microbiota.					
35980327	7	39	theme	microbiota	1247:1256	arg1	replication					1217:1227	replication	1217:1227	replication	1217:1227	The results of PICRUSt indicated that the therapeutic effects of BBH were tightly connected with DNA synthesis, replication and repair of gut microbiota.					
35980327	7	39	theme	microbiota	1247:1256	arg1	synthesis					1206:1214	DNA synthesis	1202:1214	DNA synthesis	1202:1214	The results of PICRUSt indicated that the therapeutic effects of BBH were tightly connected with DNA synthesis, replication and repair of gut microbiota.					
35980327	5	40	theme	colitis	706:712	arg1	condition					693:701	the morphological condition	675:701	the morphological condition of colitis in mice	675:720	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	7	41	theme	BBH	1170:1172	arg1	effects					1159:1165	the therapeutic effects	1143:1165	the therapeutic effects of BBH	1143:1172	The results of PICRUSt indicated that the therapeutic effects of BBH were tightly connected with DNA synthesis, replication and repair of gut microbiota.					
35980327	1	42	theme	Rhizoma	248:254	arg1	compositions					200:211	the main chemical compositions	182:211	the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma	182:254	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	1	42	theme	Rhizoma	248:254	arg1	Baicalin					141:148	Baicalin	141:148	Baicalin	141:148	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	1	42	theme	Rhizoma	248:254	arg1	hydrochloride					164:176	berberine hydrochloride	154:176	berberine hydrochloride	154:176	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	8	43	theme	protective	1353:1362	arg1	effects					1364:1370	the protective effects	1349:1370	the protective effects	1349:1370	In summary, it was concluded that BBH could protect mice against DSS-induced colitis, and the protective effects were tightly correlated with gut microbiota.					
35980327	8	44	theme	DSS-induced	1324:1334	arg1	colitis					1336:1342	DSS-induced colitis	1324:1342	DSS-induced colitis	1324:1342	In summary, it was concluded that BBH could protect mice against DSS-induced colitis, and the protective effects were tightly correlated with gut microbiota.					
35980327	4	45	theme	dextran	627:633	arg1	DSS					651:653	DSS	651:653	DSS	651:653	The results of pharmacological evaluations demonstrated the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS).					
35980327	4	45	theme	dextran	627:633	arg1	sodium					643:648	dextran sulfate sodium	627:648	dextran sulfate sodium (DSS)	627:654	The results of pharmacological evaluations demonstrated the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS).					
35980327	0	46	theme	Sulfate	74:80	arg1	Colitis					97:103	Dextran Sulfate Sodium-Induced Colitis	66:103	Dextran Sulfate Sodium-Induced Colitis	66:103	A Combination of Baicalin and Berberine Hydrochloride Ameliorates Dextran Sulfate Sodium-Induced Colitis by Modulating Colon Gut Microbiota.					
35980327	6	47	theme	Erysipelotrichaceae_UCG_003	1029:1055	arg1	abundances					977:986	the abundances	973:986	the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas	973:1102	The 16s rDNA sequencing revealed that BBH was able to modulate the composition of intestinal microflora, especially the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas.					
35980327	5	48	dep	cytokines	829:837	arg1	IL-4					840:843	IL-4	840:843	IL-4	840:843	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	48	dep	cytokines	829:837	arg1	cytokines					829:837	anti-inflammation cytokines	811:837	anti-inflammation cytokines (IL-4 and IL-10)	811:854	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	48	dep	cytokines	829:837	arg1	IL-10					849:853	IL-10	849:853	IL-10	849:853	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	1	49	theme	berberine	154:162	arg1	compositions					200:211	the main chemical compositions	182:211	the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma	182:254	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	1	49	theme	berberine	154:162	arg1	hydrochloride					164:176	berberine hydrochloride	154:176	berberine hydrochloride	154:176	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	1	49	theme	berberine	154:162	arg1	Baicalin					141:148	Baicalin	141:148	Baicalin	141:148	Baicalin and berberine hydrochloride are the main chemical compositions of Scutellariae Radix and Coptidis Rhizoma, respectively.					
35980327	5	50	from	condition	693:701	arg1	mice					717:720	mice	717:720	mice	717:720	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	0	51	theme	Dextran	66:72	arg1	Sulfate					74:80	Dextran Sulfate	66:80	Dextran Sulfate Sodium-Induced Colitis	66:103	A Combination of Baicalin and Berberine Hydrochloride Ameliorates Dextran Sulfate Sodium-Induced Colitis by Modulating Colon Gut Microbiota.					
35980327	6	52	theme	Eubacterium_brachy_group	991:1014	arg1	abundances					977:986	the abundances	973:986	the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas	973:1102	The 16s rDNA sequencing revealed that BBH was able to modulate the composition of intestinal microflora, especially the abundances of Eubacterium_brachy_group, Holdemania, Erysipelotrichaceae_UCG_003, Christensenellaceae_R-7_group, and Sellimonas.					
35980327	4	53	theme	sulfate	635:641	arg1	DSS					651:653	DSS	651:653	DSS	651:653	The results of pharmacological evaluations demonstrated the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS).					
35980327	4	53	theme	sulfate	635:641	arg1	sodium					643:648	dextran sulfate sodium	627:648	dextran sulfate sodium (DSS)	627:654	The results of pharmacological evaluations demonstrated the excellent protective effects of BBH on colitis induced by dextran sulfate sodium (DSS).					
35980327	5	54	theme	proinflammation	750:764	arg1	IL-6					777:780	IL-6	777:780	IL-6	777:780	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	54	theme	proinflammation	750:764	arg1	cytokines					766:774	proinflammation cytokines	750:774	proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α)	750:805	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	54	theme	proinflammation	750:764	arg1	IL-1β					789:793	IL-1β	789:793	IL-1β	789:793	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	54	theme	proinflammation	750:764	arg1	TNF-α					800:804	TNF-α	800:804	TNF-α	800:804	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
35980327	5	54	theme	proinflammation	750:764	arg1	IL-8					783:786	IL-8	783:786	IL-8	783:786	BBH could improve the morphological condition of colitis in mice and maintain the balance of proinflammation cytokines (IL-6, IL-8, IL-1β, and TNF-α) and anti-inflammation cytokines (IL-4 and IL-10).					
37189390	0	0	theme	Solvent	88:94	arg1	Solutions					104:112	Deep Eutectic Solvent Aqueous Solutions	74:112	Deep Eutectic Solvent Aqueous Solutions	74:112	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.					
37189390	2	1	theme	cross-linked	495:506	arg1	CLEAs					527:531	CLEAs	527:531	CLEAs	527:531	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	2	1	theme	cross-linked	495:506	arg1	aggregates					515:524	cross-linked enzyme aggregates	495:524	both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs)	464:532	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	4	2	with	comparison	957:966	arg1	up					1000:1001	the non-immobilized enzyme up	973:1001	the non-immobilized enzyme up to 3.6-fold	973:1013	The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold.					
37189390	0	3	theme	Eutectic	79:86	arg1	Solutions					104:112	Deep Eutectic Solvent Aqueous Solutions	74:112	Deep Eutectic Solvent Aqueous Solutions	74:112	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.					
37189390	3	4	theme	numerous	697:704	arg1	solutions					718:726	numerous DES aqueous solutions	697:726	numerous DES aqueous solutions	697:726	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	1	5	from	importance	323:332	arg1	field					122:126	the field	118:126	the field of biocatalysis	118:142	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	4	6	theme	enzyme	993:998	arg1	up					1000:1001	the non-immobilized enzyme up	973:1001	the non-immobilized enzyme up to 3.6-fold	973:1013	The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold.					
37189390	4	7	theme	DESs	793:796	arg1	nature					757:762	nature	757:762	nature	757:762	The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold.					
37189390	4	7	theme	DESs	793:796	arg1	concentration					772:784	the concentration	768:784	the concentration	768:784	The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold.					
37189390	7	8	theme	great	1316:1320	arg1	ability					1322:1328	great ability	1316:1328	great ability	1316:1328	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	7	9	theme	films	1475:1479	arg1	activity					1459:1466	the antioxidant activity	1443:1466	the antioxidant activity of the films	1443:1479	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	3	10	theme	DES	706:708	arg1	solutions					718:726	numerous DES aqueous solutions	697:726	numerous DES aqueous solutions	697:726	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	7	11	theme	chitosan	1358:1365	arg1	functionalization					1337:1353	the functionalization	1333:1353	the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)]	1333:1430	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	6	12	theme	Tyrosinase	1162:1171	arg1	mCLEAs					1173:1178	Tyrosinase mCLEAs	1162:1178	Tyrosinase mCLEAs	1162:1178	Tyrosinase mCLEAs were further applied in the homogeneous modification of chitosan with caffeic acid in the presence of DES.					
37189390	1	13	theme	Deep	276:279	arg1	DESs					300:303	DESs	300:303	DESs	300:303	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	1	13	theme	Deep	276:279	arg1	Solvents					290:297	Deep Eutectic Solvents	276:297	Deep Eutectic Solvents (DESs)	276:304	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	0	14	theme	Aqueous	96:102	arg1	Solutions					104:112	Deep Eutectic Solvent Aqueous Solutions	74:112	Deep Eutectic Solvent Aqueous Solutions	74:112	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.					
37189390	0	15	from	Study	65:69	arg1	Solutions					104:112	Deep Eutectic Solvent Aqueous Solutions	74:112	Deep Eutectic Solvent Aqueous Solutions	74:112	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.					
37189390	7	16	theme	DES	1412:1414	arg1	[Bet					1416:1419	10% v/v DES [Bet	1404:1419	10% v/v DES [Bet:Gly (1:3)]	1404:1430	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	1	17	theme	Eutectic	281:288	arg1	DESs					300:303	DESs	300:303	DESs	300:303	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	1	17	theme	Eutectic	281:288	arg1	Solvents					290:297	Deep Eutectic Solvents	276:297	Deep Eutectic Solvents (DESs)	276:304	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	4	18	theme	non-immobilized	977:991	arg1	up					1000:1001	the non-immobilized enzyme up	973:1001	the non-immobilized enzyme up to 3.6-fold	973:1013	The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold.					
37189390	6	19	theme	caffeic	1250:1256	arg1	acid					1258:1261	caffeic acid	1250:1261	caffeic acid in the presence of DES	1250:1284	Tyrosinase mCLEAs were further applied in the homogeneous modification of chitosan with caffeic acid in the presence of DES.					
37189390	7	20	theme	10	1404:1405	arg1	%					1406:1406	%	1406:1406	%	1406:1406	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	1	21	theme	sustainable	167:177	arg1	immobilization					204:217	enzyme immobilization	197:217	enzyme immobilization	197:217	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	1	21	theme	sustainable	167:177	arg1	processes					179:187	sustainable processes	167:187	sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs)	167:304	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	1	21	theme	sustainable	167:177	arg1	employment					222:231	employment	222:231	employment	222:231	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	7	22	theme	[Bet	1416:1419	arg1	presence					1392:1399	the presence	1388:1399	the presence of 10% v/v DES [Bet:Gly (1:3)]	1388:1430	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	3	23	theme	prepared	539:546	arg1	biocatalyst					548:558	The prepared biocatalyst	535:558	The prepared biocatalyst	535:558	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	4	24	dep	activity	855:862	arg1	the					841:843	the	841:843	the	841:843	The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold.					
37189390	1	25	theme	processes	179:187	arg1	implementation					149:162	the implementation	145:162	the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs)	145:304	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	0	26	theme	Magnetic	11:18	arg1	Aggregates					40:49	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates	0:49	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.	0:113	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.					
37189390	2	27	theme	magnetic	486:493	arg1	CLEAs					527:531	CLEAs	527:531	CLEAs	527:531	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	2	27	theme	magnetic	486:493	arg1	aggregates					515:524	cross-linked enzyme aggregates	495:524	both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs)	464:532	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	6	28	theme	chitosan	1236:1243	arg1	modification					1220:1231	the homogeneous modification	1204:1231	the homogeneous modification of chitosan with caffeic acid in the presence of DES	1204:1284	Tyrosinase mCLEAs were further applied in the homogeneous modification of chitosan with caffeic acid in the presence of DES.					
37189390	2	29	theme	aggregates	515:524	arg1	preparation					449:459	the preparation	445:459	the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs)	445:532	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	2	30	theme	fresh	379:383	arg1	mushrooms					385:393	fresh mushrooms	379:393	fresh mushrooms	379:393	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	0	31	theme	Tyrosinase	0:9	arg1	Aggregates					40:49	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates	0:49	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.	0:113	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.					
37189390	5	32	theme	repeated	1145:1152	arg1	cycles					1154:1159	5 repeated cycles	1143:1159	5 repeated cycles	1143:1159	The biocatalyst retained the 100% of its initial activity after storage at -20 °C for 1 year and the 90% of its activity after 5 repeated cycles.					
37189390	3	33	theme	biocatalytic	586:597	arg1	traits					614:619	the biocatalytic and structural traits	582:619	the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs)	582:677	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	4	34	theme	tyrosinase	881:890	arg1	stability					868:876	stability	868:876	stability	868:876	The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold.					
37189390	4	34	theme	tyrosinase	881:890	arg1	activity					855:862	catalytic activity	845:862	catalytic activity	845:862	The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold.					
37189390	0	35	theme	Enzyme	33:38	arg1	Aggregates					40:49	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates	0:49	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.	0:113	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.					
37189390	3	36	theme	structural	603:612	arg1	traits					614:619	the biocatalytic and structural traits	582:619	the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs)	582:677	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	1	37	theme	enzyme	197:202	arg1	immobilization					204:217	enzyme immobilization	197:217	enzyme immobilization	197:217	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	0	38	theme	Cross-Linked	20:31	arg1	Aggregates					40:49	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates	0:49	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.	0:113	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.					
37189390	4	39	theme	enzyme	947:952	arg1	activity					931:938	the activity	927:938	the activity of the enzyme	927:952	The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold.					
37189390	6	40	from	acid	1258:1261	arg1	presence					1270:1277	the presence	1266:1277	the presence of DES	1266:1284	Tyrosinase mCLEAs were further applied in the homogeneous modification of chitosan with caffeic acid in the presence of DES.					
37189390	7	41	theme	caffeic	1372:1378	arg1	acid					1380:1383	caffeic acid	1372:1383	caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)]	1372:1430	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	7	42	from	acid	1380:1383	arg1	presence					1392:1399	the presence	1388:1399	the presence of 10% v/v DES [Bet:Gly (1:3)]	1388:1430	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	6	43	with	modification	1220:1231	arg1	acid					1258:1261	caffeic acid	1250:1261	caffeic acid in the presence of DES	1250:1284	Tyrosinase mCLEAs were further applied in the homogeneous modification of chitosan with caffeic acid in the presence of DES.					
37189390	2	44	theme	non-magnetic	469:480	arg1	CLEAs					527:531	CLEAs	527:531	CLEAs	527:531	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	2	44	theme	non-magnetic	469:480	arg1	aggregates					515:524	cross-linked enzyme aggregates	495:524	both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs)	464:532	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	7	45	dep	[Bet	1416:1419	arg1	Gly					1421:1423	Gly	1421:1423	Gly	1421:1423	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	7	45	dep	[Bet	1416:1419	arg1	1:3					1426:1428	1:3	1426:1428	1:3	1426:1428	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	3	46	theme	tyrosinase	644:653	arg1	mCLEAs					671:676	mCLEAs	671:676	mCLEAs	671:676	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	3	46	theme	tyrosinase	644:653	arg1	CLEAs					664:668	tyrosinase magnetic CLEAs	644:668	tyrosinase magnetic CLEAs (mCLEAs)	644:677	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	3	47	theme	magnetic	655:662	arg1	mCLEAs					671:676	mCLEAs	671:676	mCLEAs	671:676	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	3	47	theme	magnetic	655:662	arg1	CLEAs					664:668	tyrosinase magnetic CLEAs	644:668	tyrosinase magnetic CLEAs (mCLEAs)	644:677	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	6	48	theme	homogeneous	1208:1218	arg1	modification					1220:1231	the homogeneous modification	1204:1231	the homogeneous modification of chitosan with caffeic acid in the presence of DES	1204:1284	Tyrosinase mCLEAs were further applied in the homogeneous modification of chitosan with caffeic acid in the presence of DES.					
37189390	2	49	link	cross-linked	495:506	arg1	CLEAs					527:531	CLEAs	527:531	CLEAs	527:531	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	2	49	link	cross-linked	495:506	arg1	aggregates					515:524	cross-linked enzyme aggregates	495:524	both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs)	464:532	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	7	50	with	functionalization	1337:1353	arg1	acid					1380:1383	caffeic acid	1372:1383	caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)]	1372:1430	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	5	51	theme	activity	1128:1135	arg1	activity					1065:1072	its initial activity	1053:1072	its initial activity after storage at -20 °C for 1 year	1053:1107	The biocatalyst retained the 100% of its initial activity after storage at -20 °C for 1 year and the 90% of its activity after 5 repeated cycles.					
37189390	5	51	theme	activity	1128:1135	arg1	%					1119:1119	the 90%	1113:1119	the 90% of its activity	1113:1135	The biocatalyst retained the 100% of its initial activity after storage at -20 °C for 1 year and the 90% of its activity after 5 repeated cycles.					
37189390	5	51	theme	activity	1128:1135	arg1	%					1048:1048	the 100%	1041:1048	the 100% of its initial activity after storage at -20 °C for 1 year	1041:1107	The biocatalyst retained the 100% of its initial activity after storage at -20 °C for 1 year and the 90% of its activity after 5 repeated cycles.					
37189390	5	51	theme	activity	1128:1135	arg1	activity					1128:1135	its activity	1124:1135	its activity	1124:1135	The biocatalyst retained the 100% of its initial activity after storage at -20 °C for 1 year and the 90% of its activity after 5 repeated cycles.					
37189390	3	52	theme	CLEAs	664:668	arg1	traits					614:619	the biocatalytic and structural traits	582:619	the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs)	582:677	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	4	53	theme	catalytic	845:853	arg1	activity					855:862	catalytic activity	845:862	catalytic activity	845:862	The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold.					
37189390	0	54	theme	Biocatalytic	52:63	arg1	Study					65:69	Biocatalytic Study	52:69	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.	0:113	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.					
37189390	1	55	theme	paramount	313:321	arg1	importance					323:332	paramount importance	313:332	paramount importance	313:332	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	7	56	theme	antioxidant	1447:1457	arg1	activity					1459:1466	the antioxidant activity	1443:1466	the antioxidant activity of the films	1443:1479	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	0	57	dep	Aggregates	40:49	arg1	Study					65:69	Biocatalytic Study	52:69	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.	0:113	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.					
37189390	4	58	dep	nature	757:762	arg1	the					753:755	the	753:755	the	753:755	The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold.					
37189390	7	59	theme	v/v	1408:1410	arg1	[Bet					1416:1419	10% v/v DES [Bet	1404:1419	10% v/v DES [Bet:Gly (1:3)]	1404:1430	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	6	60	theme	DES	1282:1284	arg1	presence					1270:1277	the presence	1266:1277	the presence of DES	1266:1284	Tyrosinase mCLEAs were further applied in the homogeneous modification of chitosan with caffeic acid in the presence of DES.					
37189390	5	61	theme	initial	1057:1063	arg1	activity					1065:1072	its initial activity	1053:1072	its initial activity after storage at -20 °C for 1 year	1053:1107	The biocatalyst retained the 100% of its initial activity after storage at -20 °C for 1 year and the 90% of its activity after 5 repeated cycles.					
37189390	7	62	theme	%	1406:1406	arg1	v/v					1408:1410	10% v/v	1404:1410	10% v/v DES [Bet:Gly (1:3)]	1404:1430	The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.					
37189390	5	63	theme	activity	1065:1072	arg1	activity					1065:1072	its initial activity	1053:1072	its initial activity after storage at -20 °C for 1 year	1053:1107	The biocatalyst retained the 100% of its initial activity after storage at -20 °C for 1 year and the 90% of its activity after 5 repeated cycles.					
37189390	5	63	theme	activity	1065:1072	arg1	%					1119:1119	the 90%	1113:1119	the 90% of its activity	1113:1135	The biocatalyst retained the 100% of its initial activity after storage at -20 °C for 1 year and the 90% of its activity after 5 repeated cycles.					
37189390	5	63	theme	activity	1065:1072	arg1	%					1048:1048	the 100%	1041:1048	the 100% of its initial activity after storage at -20 °C for 1 year	1041:1107	The biocatalyst retained the 100% of its initial activity after storage at -20 °C for 1 year and the 90% of its activity after 5 repeated cycles.					
37189390	5	63	theme	activity	1065:1072	arg1	activity					1128:1135	its activity	1124:1135	its activity	1124:1135	The biocatalyst retained the 100% of its initial activity after storage at -20 °C for 1 year and the 90% of its activity after 5 repeated cycles.					
37189390	2	64	used	used	399:402	arg2	tyrosinase					349:358	tyrosinase	349:358	tyrosinase	349:358	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	1	65	theme	biocatalysis	131:142	arg1	field					122:126	the field	118:126	the field of biocatalysis	118:142	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	5	66	from	°C	1095:1096	arg1	storage					1080:1086	storage	1080:1086	storage at -20 °C for 1 year	1080:1107	The biocatalyst retained the 100% of its initial activity after storage at -20 °C for 1 year and the 90% of its activity after 5 repeated cycles.					
37189390	1	67	theme	environmentally	236:250	arg1	solvents					261:268	environmentally friendly solvents	236:268	environmentally friendly solvents	236:268	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	0	68	theme	Deep	74:77	arg1	Solutions					104:112	Deep Eutectic Solvent Aqueous Solutions	74:112	Deep Eutectic Solvent Aqueous Solutions	74:112	Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.					
37189390	3	69	theme	free	624:627	arg1	tyrosinase					629:638	free tyrosinase	624:638	free tyrosinase	624:638	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	2	70	theme	carrier-free	409:420	arg1	immobilization					422:435	a carrier-free immobilization	407:435	a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs)	407:532	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	1	71	theme	friendly	252:259	arg1	solvents					261:268	environmentally friendly solvents	236:268	environmentally friendly solvents	236:268	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	2	72	theme	enzyme	508:513	arg1	CLEAs					527:531	CLEAs	527:531	CLEAs	527:531	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	2	72	theme	enzyme	508:513	arg1	aggregates					515:524	cross-linked enzyme aggregates	495:524	both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs)	464:532	In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs).					
37189390	3	73	theme	aqueous	710:716	arg1	solutions					718:726	numerous DES aqueous solutions	697:726	numerous DES aqueous solutions	697:726	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	3	74	theme	tyrosinase	629:638	arg1	traits					614:619	the biocatalytic and structural traits	582:619	the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs)	582:677	The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions.					
37189390	1	75	theme	solvents	261:268	arg1	immobilization					204:217	enzyme immobilization	197:217	enzyme immobilization	197:217	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
37189390	1	75	theme	solvents	261:268	arg1	employment					222:231	employment	222:231	employment	222:231	In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance.					
35997573	9	0	theme	complexes	1704:1712	arg1	formation					1681:1689	the formation	1677:1689	the formation of polymeric complexes in the presence of chitin	1677:1738	Interestingly, the intermolecular LysM dimerization allows for the formation of polymeric complexes in the presence of chitin.					
35997573	5	1	dep	Verticillium	1048:1059	arg1	dahliae					1061:1067	dahliae	1061:1067	dahliae	1061:1067	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	4	2	theme	composite	868:876	arg1	groove					878:883	a composite groove	866:883	a composite groove with ultra-high (pM) chitin-binding affinity	866:928	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	5	3	contain	contain	987:993	arg2	ChElp2					1121:1126	ChElp2	1121:1126	ChElp2	1121:1126	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	5	3	contain	contain	987:993	arg2	Vd2LysM					1069:1075	Verticillium dahliae Vd2LysM	1048:1075	Verticillium dahliae Vd2LysM	1048:1075	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	5	3	contain	contain	987:993	arg1	effectors					977:985	most functionally characterized LysM effectors	940:985	most functionally characterized LysM effectors	940:985	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	5	3	contain	contain	987:993	arg2	LysMs					1004:1008	only two LysMs	995:1008	only two LysMs	995:1008	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	5	3	contain	contain	987:993	arg2	MoSlp1					1040:1045	Magnaporthe oryzae MoSlp1	1021:1045	Magnaporthe oryzae MoSlp1	1021:1045	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	5	3	contain	contain	987:993	arg2	ChElp1					1110:1115	Colletotrichum higginsianum ChElp1	1082:1115	Colletotrichum higginsianum ChElp1	1082:1115	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	6	4	theme	affinity	1289:1296	arg1	grooves					1298:1304	ultra-high chitin-binding affinity grooves	1263:1304	ultra-high chitin-binding affinity grooves	1263:1304	Here, we performed modeling, structural, and functional analyses to investigate whether such dual-domain LysM effectors can also form ultra-high chitin-binding affinity grooves through intramolecular LysM dimerization.					
35997573	10	5	theme	chitin	1813:1818	arg1	oligomers					1820:1828	chitin oligomers	1813:1828	chitin oligomers	1813:1828	Possibly, such polymers may precipitate at infection sites to eliminate chitin oligomers, and thus suppress the activation of chitin-induced plant immunity.					
35997573	4	6	theme	LysM	835:838	arg1	domains					840:846	three LysM domains	829:846	its three LysM domains	825:846	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	6	7	theme	ultra-high	1263:1272	arg1	grooves					1298:1304	ultra-high chitin-binding affinity grooves	1263:1304	ultra-high chitin-binding affinity grooves	1263:1304	Here, we performed modeling, structural, and functional analyses to investigate whether such dual-domain LysM effectors can also form ultra-high chitin-binding affinity grooves through intramolecular LysM dimerization.					
35997573	3	8	theme	chitin-induced	501:514	arg1	immunity					522:529	chitin-induced plant immunity	501:529	chitin-induced plant immunity	501:529	To deregulate chitin-induced plant immunity and successfully establish infection, many fungal pathogens secrete LysM domain-containing effector proteins during host colonization.					
35997573	6	9	theme	structural	1158:1167	arg1	analyses					1185:1192	modeling, structural, and functional analyses	1148:1192	modeling, structural, and functional analyses	1148:1192	Here, we performed modeling, structural, and functional analyses to investigate whether such dual-domain LysM effectors can also form ultra-high chitin-binding affinity grooves through intramolecular LysM dimerization.					
35997573	4	10	theme	effector	675:682	arg1	Ecp6					684:687	The LysM effector Ecp6	666:687	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum	666:763	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	2	11	theme	molecular	287:295	arg1	chitin					251:256	chitin	251:256	chitin	251:256	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	2	11	theme	molecular	287:295	arg1	MAMP					306:309	MAMP	306:309	MAMP	306:309	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	2	11	theme	molecular	287:295	arg1	pattern					297:303	a microbe-associated molecular pattern	266:303	a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses	266:484	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	6	12	theme	modeling	1148:1155	arg1	analyses					1185:1192	modeling, structural, and functional analyses	1148:1192	modeling, structural, and functional analyses	1148:1192	Here, we performed modeling, structural, and functional analyses to investigate whether such dual-domain LysM effectors can also form ultra-high chitin-binding affinity grooves through intramolecular LysM dimerization.					
35997573	8	13	theme	intermolecular	1473:1486	arg1	dimerization					1493:1504	intermolecular LysM dimerization	1473:1504	intermolecular LysM dimerization	1473:1504	Rather, our data support the occurrence of intermolecular LysM dimerization for these effectors, associated with a substantially lower chitin binding affinity than monitored for Ecp6.					
35997573	1	14	theme	structural	197:206	arg1	component					208:216	a major structural component	189:216	a major structural component of fungal cell walls	189:237	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	8	15	theme	dimerization	1493:1504	arg1	occurrence					1459:1468	the occurrence	1455:1468	the occurrence of intermolecular LysM dimerization for these effectors	1455:1524	Rather, our data support the occurrence of intermolecular LysM dimerization for these effectors, associated with a substantially lower chitin binding affinity than monitored for Ecp6.					
35997573	6	16	theme	LysM	1234:1237	arg1	effectors					1239:1247	such dual-domain LysM effectors	1217:1247	such dual-domain LysM effectors	1217:1247	Here, we performed modeling, structural, and functional analyses to investigate whether such dual-domain LysM effectors can also form ultra-high chitin-binding affinity grooves through intramolecular LysM dimerization.					
35997573	3	17	theme	many	569:572	arg1	pathogens					581:589	many fungal pathogens	569:589	many fungal pathogens	569:589	To deregulate chitin-induced plant immunity and successfully establish infection, many fungal pathogens secrete LysM domain-containing effector proteins during host colonization.					
35997573	4	18	dep	fungus	738:743	arg1	lycopersicum					714:725	Solanum lycopersicum	706:725	Solanum lycopersicum	706:725	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	7	19	theme	LysM	1396:1399	arg1	dimerization					1401:1412	intramolecular LysM dimerization	1381:1412	intramolecular LysM dimerization	1381:1412	However, our study suggests that intramolecular LysM dimerization does not occur.					
35997573	6	20	theme	such	1217:1220	arg1	effectors					1239:1247	such dual-domain LysM effectors	1217:1247	such dual-domain LysM effectors	1217:1247	Here, we performed modeling, structural, and functional analyses to investigate whether such dual-domain LysM effectors can also form ultra-high chitin-binding affinity grooves through intramolecular LysM dimerization.					
35997573	6	21	theme	LysM	1329:1332	arg1	dimerization					1334:1345	intramolecular LysM dimerization	1314:1345	intramolecular LysM dimerization	1314:1345	Here, we performed modeling, structural, and functional analyses to investigate whether such dual-domain LysM effectors can also form ultra-high chitin-binding affinity grooves through intramolecular LysM dimerization.					
35997573	5	22	theme	Verticillium	1048:1059	arg1	Vd2LysM					1069:1075	Verticillium dahliae Vd2LysM	1048:1075	Verticillium dahliae Vd2LysM	1048:1075	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	3	23	theme	effector	622:629	arg1	proteins					631:638	LysM domain-containing effector proteins	599:638	LysM domain-containing effector proteins	599:638	To deregulate chitin-induced plant immunity and successfully establish infection, many fungal pathogens secrete LysM domain-containing effector proteins during host colonization.					
35997573	2	24	theme	downstream	458:467	arg1	responses					476:484	downstream immune responses	458:484	downstream immune responses	458:484	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	10	25	theme	plant	1882:1886	arg1	immunity					1888:1895	chitin-induced plant immunity	1867:1895	chitin-induced plant immunity	1867:1895	Possibly, such polymers may precipitate at infection sites to eliminate chitin oligomers, and thus suppress the activation of chitin-induced plant immunity.					
35997573	4	26	theme	tomato	698:703	arg1	fungus					738:743	the tomato (Solanum lycopersicum) leaf mold fungus	694:743	the tomato (Solanum lycopersicum) leaf mold fungus	694:743	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	0	27	theme	intramolecular	81:94	arg1	dimerization					97:108	chitin-induced intermolecular, and not intramolecular, dimerization	42:108	chitin-induced intermolecular, and not intramolecular, dimerization	42:108	Fungal dual-domain LysM effectors undergo chitin-induced intermolecular, and not intramolecular, dimerization.					
35997573	1	28	theme	-linked	145:151	arg1	GlcNAc					177:182	GlcNAc	177:182	GlcNAc	177:182	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	1	28	theme	-linked	145:151	arg1	N-acetyl-D-glucosamine					153:174	β-(1,4)-linked N-acetyl-D-glucosamine	138:174	β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc)	138:183	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	5	29	theme	characterized	958:970	arg1	effectors					977:985	most functionally characterized LysM effectors	940:985	most functionally characterized LysM effectors	940:985	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	2	30	theme	responses	476:484	arg1	plethora					446:453	a plethora	444:453	a plethora of downstream immune responses	444:484	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	8	31	theme	chitin	1565:1570	arg1	affinity					1580:1587	a substantially lower chitin binding affinity	1543:1587	a substantially lower chitin binding affinity than monitored for Ecp6	1543:1611	Rather, our data support the occurrence of intermolecular LysM dimerization for these effectors, associated with a substantially lower chitin binding affinity than monitored for Ecp6.					
35997573	4	32	theme	plant	780:784	arg1	PRRs					786:789	plant PRRs	780:789	plant PRRs for chitin binding	780:808	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	4	33	theme	mold	733:736	arg1	fungus					738:743	the tomato (Solanum lycopersicum) leaf mold fungus	694:743	the tomato (Solanum lycopersicum) leaf mold fungus	694:743	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	0	34	theme	Fungal	0:5	arg1	effectors					24:32	Fungal dual-domain LysM effectors	0:32	Fungal dual-domain LysM effectors	0:32	Fungal dual-domain LysM effectors undergo chitin-induced intermolecular, and not intramolecular, dimerization.					
35997573	2	35	theme	recognition	401:411	arg1	receptors					413:421	lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors	334:421	lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses	334:484	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	2	35	theme	recognition	401:411	arg1	PRRs					424:427	PRRs	424:427	PRRs	424:427	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	2	36	theme	surface-localized	375:391	arg1	receptors					413:421	lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors	334:421	lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses	334:484	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	2	36	theme	surface-localized	375:391	arg1	PRRs					424:427	PRRs	424:427	PRRs	424:427	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	0	37	theme	LysM	19:22	arg1	effectors					24:32	Fungal dual-domain LysM effectors	0:32	Fungal dual-domain LysM effectors	0:32	Fungal dual-domain LysM effectors undergo chitin-induced intermolecular, and not intramolecular, dimerization.					
35997573	9	38	theme	intermolecular	1633:1646	arg1	dimerization					1653:1664	the intermolecular LysM dimerization	1629:1664	the intermolecular LysM dimerization	1629:1664	Interestingly, the intermolecular LysM dimerization allows for the formation of polymeric complexes in the presence of chitin.					
35997573	2	39	theme	plant	364:368	arg1	receptors					413:421	lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors	334:421	lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses	334:484	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	2	39	theme	plant	364:368	arg1	PRRs					424:427	PRRs	424:427	PRRs	424:427	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	0	40	theme	intermolecular	57:70	arg1	dimerization					97:108	chitin-induced intermolecular, and not intramolecular, dimerization	42:108	chitin-induced intermolecular, and not intramolecular, dimerization	42:108	Fungal dual-domain LysM effectors undergo chitin-induced intermolecular, and not intramolecular, dimerization.					
35997573	5	41	theme	oryzae	1033:1038	arg1	MoSlp1					1040:1045	Magnaporthe oryzae MoSlp1	1021:1045	Magnaporthe oryzae MoSlp1	1021:1045	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	3	42	theme	LysM	599:602	arg1	proteins					631:638	LysM domain-containing effector proteins	599:638	LysM domain-containing effector proteins	599:638	To deregulate chitin-induced plant immunity and successfully establish infection, many fungal pathogens secrete LysM domain-containing effector proteins during host colonization.					
35997573	10	43	theme	such	1751:1754	arg1	polymers					1756:1763	such polymers	1751:1763	such polymers	1751:1763	Possibly, such polymers may precipitate at infection sites to eliminate chitin oligomers, and thus suppress the activation of chitin-induced plant immunity.					
35997573	5	44	dep	Colletotrichum	1082:1095	arg1	higginsianum					1097:1108	higginsianum	1097:1108	higginsianum	1097:1108	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	1	45	theme	fungal	221:226	arg1	walls					233:237	fungal cell walls	221:237	fungal cell walls	221:237	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	9	46	theme	polymeric	1694:1702	arg1	complexes					1704:1712	polymeric complexes	1694:1712	polymeric complexes	1694:1712	Interestingly, the intermolecular LysM dimerization allows for the formation of polymeric complexes in the presence of chitin.					
35997573	6	47	theme	intramolecular	1314:1327	arg1	dimerization					1334:1345	intramolecular LysM dimerization	1314:1345	intramolecular LysM dimerization	1314:1345	Here, we performed modeling, structural, and functional analyses to investigate whether such dual-domain LysM effectors can also form ultra-high chitin-binding affinity grooves through intramolecular LysM dimerization.					
35997573	1	48	theme	walls	233:237	arg1	component					208:216	a major structural component	189:216	a major structural component of fungal cell walls	189:237	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	1	48	theme	walls	233:237	arg1	Chitin					111:116	Chitin	111:116	Chitin	111:116	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	1	48	theme	walls	233:237	arg1	homopolymer					123:133	a homopolymer	121:133	a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc)	121:183	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	3	49	theme	plant	516:520	arg1	immunity					522:529	chitin-induced plant immunity	501:529	chitin-induced plant immunity	501:529	To deregulate chitin-induced plant immunity and successfully establish infection, many fungal pathogens secrete LysM domain-containing effector proteins during host colonization.					
35997573	6	50	theme	functional	1174:1183	arg1	analyses					1185:1192	modeling, structural, and functional analyses	1148:1192	modeling, structural, and functional analyses	1148:1192	Here, we performed modeling, structural, and functional analyses to investigate whether such dual-domain LysM effectors can also form ultra-high chitin-binding affinity grooves through intramolecular LysM dimerization.					
35997573	6	51	theme	chitin-binding	1274:1287	arg1	grooves					1298:1304	ultra-high chitin-binding affinity grooves	1263:1304	ultra-high chitin-binding affinity grooves	1263:1304	Here, we performed modeling, structural, and functional analyses to investigate whether such dual-domain LysM effectors can also form ultra-high chitin-binding affinity grooves through intramolecular LysM dimerization.					
35997573	1	52	theme	N-acetyl-D-glucosamine	153:174	arg1	component					208:216	a major structural component	189:216	a major structural component of fungal cell walls	189:237	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	1	52	theme	N-acetyl-D-glucosamine	153:174	arg1	Chitin					111:116	Chitin	111:116	Chitin	111:116	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	1	52	theme	N-acetyl-D-glucosamine	153:174	arg1	homopolymer					123:133	a homopolymer	121:133	a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc)	121:183	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	9	53	theme	chitin	1733:1738	arg1	presence					1721:1728	the presence	1717:1728	the presence of chitin	1717:1738	Interestingly, the intermolecular LysM dimerization allows for the formation of polymeric complexes in the presence of chitin.					
35997573	4	54	theme	domains	840:846	arg1	domains					840:846	three LysM domains	829:846	its three LysM domains	825:846	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	4	54	theme	domains	840:846	arg1	two					818:820	two	818:820	two	818:820	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	4	55	theme	LysM	670:673	arg1	Ecp6					684:687	The LysM effector Ecp6	666:687	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum	666:763	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	2	56	theme	microbe-associated	268:285	arg1	chitin					251:256	chitin	251:256	chitin	251:256	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	2	56	theme	microbe-associated	268:285	arg1	MAMP					306:309	MAMP	306:309	MAMP	306:309	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	2	56	theme	microbe-associated	268:285	arg1	pattern					297:303	a microbe-associated molecular pattern	266:303	a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses	266:484	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	8	57	theme	LysM	1488:1491	arg1	dimerization					1493:1504	intermolecular LysM dimerization	1473:1504	intermolecular LysM dimerization	1473:1504	Rather, our data support the occurrence of intermolecular LysM dimerization for these effectors, associated with a substantially lower chitin binding affinity than monitored for Ecp6.					
35997573	3	58	theme	fungal	574:579	arg1	pathogens					581:589	many fungal pathogens	569:589	many fungal pathogens	569:589	To deregulate chitin-induced plant immunity and successfully establish infection, many fungal pathogens secrete LysM domain-containing effector proteins during host colonization.					
35997573	1	59	theme	major	191:195	arg1	component					208:216	a major structural component	189:216	a major structural component of fungal cell walls	189:237	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	4	60	theme	chitin-binding	906:919	arg1	affinity					921:928	ultra-high (pM) chitin-binding affinity	890:928	ultra-high (pM) chitin-binding affinity	890:928	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	5	61	theme	Colletotrichum	1082:1095	arg1	ChElp1					1110:1115	Colletotrichum higginsianum ChElp1	1082:1115	Colletotrichum higginsianum ChElp1	1082:1115	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	6	62	theme	dual-domain	1222:1232	arg1	effectors					1239:1247	such dual-domain LysM effectors	1217:1247	such dual-domain LysM effectors	1217:1247	Here, we performed modeling, structural, and functional analyses to investigate whether such dual-domain LysM effectors can also form ultra-high chitin-binding affinity grooves through intramolecular LysM dimerization.					
35997573	3	63	theme	domain-containing	604:620	arg1	proteins					631:638	LysM domain-containing effector proteins	599:638	LysM domain-containing effector proteins	599:638	To deregulate chitin-induced plant immunity and successfully establish infection, many fungal pathogens secrete LysM domain-containing effector proteins during host colonization.					
35997573	10	64	theme	immunity	1888:1895	arg1	activation					1853:1862	the activation	1849:1862	the activation of chitin-induced plant immunity	1849:1895	Possibly, such polymers may precipitate at infection sites to eliminate chitin oligomers, and thus suppress the activation of chitin-induced plant immunity.					
35997573	7	65	theme	intramolecular	1381:1394	arg1	dimerization					1401:1412	intramolecular LysM dimerization	1381:1412	intramolecular LysM dimerization	1381:1412	However, our study suggests that intramolecular LysM dimerization does not occur.					
35997573	8	66	theme	lower	1559:1563	arg1	affinity					1580:1587	a substantially lower chitin binding affinity	1543:1587	a substantially lower chitin binding affinity than monitored for Ecp6	1543:1611	Rather, our data support the occurrence of intermolecular LysM dimerization for these effectors, associated with a substantially lower chitin binding affinity than monitored for Ecp6.					
35997573	8	67	theme	binding	1572:1578	arg1	affinity					1580:1587	a substantially lower chitin binding affinity	1543:1587	a substantially lower chitin binding affinity than monitored for Ecp6	1543:1611	Rather, our data support the occurrence of intermolecular LysM dimerization for these effectors, associated with a substantially lower chitin binding affinity than monitored for Ecp6.					
35997573	10	68	dep	precipitate	1769:1779	arg1	suppress					1840:1847	suppress	1840:1847	suppress the activation of chitin-induced plant immunity	1840:1895	Possibly, such polymers may precipitate at infection sites to eliminate chitin oligomers, and thus suppress the activation of chitin-induced plant immunity.					
35997573	5	69	theme	LysM	972:975	arg1	effectors					977:985	most functionally characterized LysM effectors	940:985	most functionally characterized LysM effectors	940:985	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	2	70	theme	immune	469:474	arg1	responses					476:484	downstream immune responses	458:484	downstream immune responses	458:484	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	0	71	theme	dual-domain	7:17	arg1	effectors					24:32	Fungal dual-domain LysM effectors	0:32	Fungal dual-domain LysM effectors	0:32	Fungal dual-domain LysM effectors undergo chitin-induced intermolecular, and not intramolecular, dimerization.					
35997573	4	72	from	fungus	738:743	arg1	Ecp6					684:687	The LysM effector Ecp6	666:687	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum	666:763	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	9	73	from	formation	1681:1689	arg1	presence					1721:1728	the presence	1717:1728	the presence of chitin	1717:1738	Interestingly, the intermolecular LysM dimerization allows for the formation of polymeric complexes in the presence of chitin.					
35997573	4	74	theme	leaf	728:731	arg1	fungus					738:743	the tomato (Solanum lycopersicum) leaf mold fungus	694:743	the tomato (Solanum lycopersicum) leaf mold fungus	694:743	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	10	75	theme	chitin-induced	1867:1880	arg1	immunity					1888:1895	chitin-induced plant immunity	1867:1895	chitin-induced plant immunity	1867:1895	Possibly, such polymers may precipitate at infection sites to eliminate chitin oligomers, and thus suppress the activation of chitin-induced plant immunity.					
35997573	2	76	theme	pattern	393:399	arg1	receptors					413:421	lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors	334:421	lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses	334:484	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	2	76	theme	pattern	393:399	arg1	PRRs					424:427	PRRs	424:427	PRRs	424:427	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	1	77	link	-linked	145:151	arg1	GlcNAc					177:182	GlcNAc	177:182	GlcNAc	177:182	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	1	77	link	-linked	145:151	arg1	N-acetyl-D-glucosamine					153:174	β-(1,4)-linked N-acetyl-D-glucosamine	138:174	β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc)	138:183	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
35997573	3	78	theme	host	647:650	arg1	colonization					652:663	host colonization	647:663	host colonization	647:663	To deregulate chitin-induced plant immunity and successfully establish infection, many fungal pathogens secrete LysM domain-containing effector proteins during host colonization.					
35997573	2	79	theme	cell	370:373	arg1	receptors					413:421	lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors	334:421	lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses	334:484	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	2	79	theme	cell	370:373	arg1	PRRs					424:427	PRRs	424:427	PRRs	424:427	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	0	80	theme	chitin-induced	42:55	arg1	dimerization					97:108	chitin-induced intermolecular, and not intramolecular, dimerization	42:108	chitin-induced intermolecular, and not intramolecular, dimerization	42:108	Fungal dual-domain LysM effectors undergo chitin-induced intermolecular, and not intramolecular, dimerization.					
35997573	9	81	theme	LysM	1648:1651	arg1	dimerization					1653:1664	the intermolecular LysM dimerization	1629:1664	the intermolecular LysM dimerization	1629:1664	Interestingly, the intermolecular LysM dimerization allows for the formation of polymeric complexes in the presence of chitin.					
35997573	4	82	with	groove	878:883	arg1	affinity					921:928	ultra-high (pM) chitin-binding affinity	890:928	ultra-high (pM) chitin-binding affinity	890:928	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	2	83	theme	-containing	352:362	arg1	receptors					413:421	lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors	334:421	lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses	334:484	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	2	83	theme	-containing	352:362	arg1	PRRs					424:427	PRRs	424:427	PRRs	424:427	In plants, chitin acts as a microbe-associated molecular pattern (MAMP) that is recognized by lysin motif (LysM)-containing plant cell surface-localized pattern recognition receptors (PRRs) that activate a plethora of downstream immune responses.					
35997573	5	84	theme	Magnaporthe	1021:1031	arg1	MoSlp1					1040:1045	Magnaporthe oryzae MoSlp1	1021:1045	Magnaporthe oryzae MoSlp1	1021:1045	However, most functionally characterized LysM effectors contain only two LysMs, including Magnaporthe oryzae MoSlp1, Verticillium dahliae Vd2LysM, and Colletotrichum higginsianum ChElp1 and ChElp2.					
35997573	8	85	dep	affinity	1580:1587	arg1	monitored					1594:1602	monitored	1594:1602	monitored for Ecp6	1594:1611	Rather, our data support the occurrence of intermolecular LysM dimerization for these effectors, associated with a substantially lower chitin binding affinity than monitored for Ecp6.					
35997573	4	86	theme	chitin	795:800	arg1	binding					802:808	chitin binding	795:808	chitin binding	795:808	The LysM effector Ecp6 from the tomato (Solanum lycopersicum) leaf mold fungus Cladosporium fulvum can outcompete plant PRRs for chitin binding because two of its three LysM domains cooperate to form a composite groove with ultra-high (pM) chitin-binding affinity.					
35997573	10	87	theme	infection	1784:1792	arg1	sites					1794:1798	infection sites	1784:1798	infection sites	1784:1798	Possibly, such polymers may precipitate at infection sites to eliminate chitin oligomers, and thus suppress the activation of chitin-induced plant immunity.					
35997573	1	88	theme	cell	228:231	arg1	walls					233:237	fungal cell walls	221:237	fungal cell walls	221:237	Chitin is a homopolymer of β-(1,4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major structural component of fungal cell walls.					
36405758	9	0	theme	related	1091:1097	arg1	genes					1209:1213	type I IFN genes	1198:1213	type I IFN genes (ifnφ1, ifnφ2, ifnφ3)	1198:1235	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	9	0	theme	related	1091:1097	arg1	mavs					1355:1358	mavs	1355:1358	mavs	1355:1358	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	9	0	theme	related	1091:1097	arg1	genes					1085:1089	genes	1085:1089	genes	1085:1089	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	9	0	theme	related	1091:1097	arg1	rig1					1349:1352	rig1	1349:1352	rig1	1349:1352	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	9	0	theme	related	1091:1097	arg1	tlr7					1343:1346	tlr7	1343:1346	tlr7	1343:1346	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	9	0	theme	related	1091:1097	arg1	irf3					1361:1364	irf3	1361:1364	irf3	1361:1364	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	9	0	theme	related	1091:1097	arg1	irf7					1370:1373	irf7	1370:1373	irf7	1370:1373	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	16	1	theme	zebrafish	2224:2232	arg1	resistance					2210:2219	resistance	2210:2219	resistance of zebrafish against SVCV	2210:2245	To sum up, our results indicate that the β-glucan enhanced resistance of zebrafish against SVCV and the mechanism involved stimulation of type I IFN antiviral immune response of fish after viral infection.					
36405758	9	2	theme	type	1102:1105	arg1	IFN					1109:1111	type I IFN	1102:1111	type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection	1102:1185	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	10	3	theme	immune	1577:1582	arg1	response					1584:1591	IFN immune response	1573:1591	IFN immune response	1573:1591	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	8	4	theme	0.025	898:902	arg1	%					903:903	%	903:903	%	903:903	Results showed that dietary supplementation of 0.025% β-glucan significantly increased survival rate of zebrafish compared with control group after SVCV challenge (P < 0.05).					
36405758	9	5	theme	IFN	1109:1111	arg1	pathway					1130:1136	type I IFN antiviral immune pathway	1102:1136	type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection	1102:1185	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	9	6	theme	other	1282:1286	arg1	genes					1288:1292	other genes	1282:1292	other genes involved in the IFN signaling pathway	1282:1330	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	8	7	theme	β-glucan	905:912	arg1	supplementation					879:893	dietary supplementation	871:893	dietary supplementation of 0.025% β-glucan	871:912	Results showed that dietary supplementation of 0.025% β-glucan significantly increased survival rate of zebrafish compared with control group after SVCV challenge (P < 0.05).					
36405758	10	8	theme	IFN	1407:1409	arg1	receptors					1411:1419	type I IFN receptors	1400:1419	type I IFN receptors	1400:1419	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	9	9	theme	immune	1123:1128	arg1	pathway					1130:1136	type I IFN antiviral immune pathway	1102:1136	type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection	1102:1185	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	10	10	theme	type	1400:1403	arg1	IFN					1407:1409	type I IFN	1400:1409	type I IFN receptors	1400:1419	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	10	11	theme	antiviral	1434:1442	arg1	effect					1444:1449	the antiviral effect	1430:1449	the antiviral effect of β-glucan in zebrafish larvae	1430:1481	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	0	12	theme	virus	122:126	arg1	infection					128:136	carp virus infection	117:136	carp virus infection	117:136	The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection.					
36405758	3	13	from	effects	315:321	arg1	resistance					342:351	the resistance	338:351	the resistance of zebrafish against spring viraemia of carp virus (SVCV)	338:409	In this study, we investigated the effects of β-glucan on the resistance of zebrafish against spring viraemia of carp virus (SVCV) and elucidated the underlying mechanisms.					
36405758	9	14	theme	signaling	1314:1322	arg1	pathway					1324:1330	the IFN signaling pathway	1306:1330	the IFN signaling pathway	1306:1330	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	12	15	theme	antiviral	1754:1762	arg1	independent					1794:1804	independent	1794:1804	independent	1794:1804	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	12	15	theme	antiviral	1754:1762	arg1	effect					1764:1769	the antiviral effect	1750:1769	the antiviral effect of β-glucan in ZF4	1750:1788	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	14	16	from	effect	1939:1944	arg1	cells					1965:1969	ZF4 cells	1961:1969	ZF4 cells	1961:1969	Inhibition of autophagy blocked the antiviral effect of β-glucan in ZF4 cells.					
36405758	9	17	dep	mxb	1260:1262	arg1	mxc					1265:1267	mxc	1265:1267	mxc	1265:1267	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	5	18	theme	embryonic	596:604	arg1	cells					623:627	Zebrafish embryonic fibroblast (ZF4) cells	586:627	Zebrafish embryonic fibroblast (ZF4) cells	586:627	Zebrafish embryonic fibroblast (ZF4) cells were treated with 5 μg/mL β-glucan and were infected by SVCV.					
36405758	9	19	theme	zebrafish	1155:1163	arg1	spleen					1145:1150	the spleen	1141:1150	the spleen of zebrafish after viral infection	1141:1185	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	15	20	theme	intestinal	2024:2033	arg1	microbiota					2035:2044	intestinal microbiota	2024:2044	intestinal microbiota	2024:2044	Lastly, dietary β-glucan changed the composition of intestinal microbiota in zebrafish, with reduced abundance of Proteobacteria and an enrichment of Fusobacteria and Firmicutes.					
36405758	9	21	theme	viral	1171:1175	arg1	infection					1177:1185	viral infection	1171:1185	viral infection	1171:1185	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	2	22	theme	viral	232:236	arg1	infections					238:247	viral infections	232:247	viral infections	232:247	However, the effect of yeast β-glucan on viral infections has been less studied in fish.					
36405758	16	23	theme	viral	2340:2344	arg1	infection					2346:2354	viral infection	2340:2354	viral infection	2340:2354	To sum up, our results indicate that the β-glucan enhanced resistance of zebrafish against SVCV and the mechanism involved stimulation of type I IFN antiviral immune response of fish after viral infection.					
36405758	13	24	theme	ZF4	1861:1863	arg1	cells					1865:1869	ZF4 cells	1861:1869	ZF4 cells	1861:1869	Interestingly, β-glucan induced autophagy in ZF4 cells after SVCV infection.					
36405758	2	25	theme	β-glucan	220:227	arg1	effect					204:209	the effect	200:209	the effect of yeast β-glucan on viral infections	200:247	However, the effect of yeast β-glucan on viral infections has been less studied in fish.					
36405758	12	26	from	response	1723:1730	arg1	cells					1739:1743	ZF4 cells	1735:1743	ZF4 cells	1735:1743	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	14	27	theme	antiviral	1929:1937	arg1	effect					1939:1944	the antiviral effect	1925:1944	the antiviral effect of β-glucan in ZF4 cells	1925:1969	Inhibition of autophagy blocked the antiviral effect of β-glucan in ZF4 cells.					
36405758	9	28	theme	type	1198:1201	arg1	IFN					1205:1207	type I IFN	1198:1207	type I IFN genes (ifnφ1, ifnφ2, ifnφ3)	1198:1235	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	15	29	theme	dietary	1980:1986	arg1	β-glucan					1988:1995	dietary β-glucan	1980:1995	dietary β-glucan	1980:1995	Lastly, dietary β-glucan changed the composition of intestinal microbiota in zebrafish, with reduced abundance of Proteobacteria and an enrichment of Fusobacteria and Firmicutes.					
36405758	0	30	theme	zebrafish	81:89	arg1	resistance					67:76	antiviral resistance	57:76	antiviral resistance of zebrafish against spring viremia of carp virus infection	57:136	The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection.					
36405758	9	31	theme	IFN	1205:1207	arg1	genes					1209:1213	type I IFN genes	1198:1213	type I IFN genes (ifnφ1, ifnφ2, ifnφ3)	1198:1235	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	12	32	theme	ZF4	1735:1737	arg1	cells					1739:1743	ZF4 cells	1735:1743	ZF4 cells	1735:1743	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	2	33	from	effect	204:209	arg1	infections					238:247	viral infections	232:247	viral infections	232:247	However, the effect of yeast β-glucan on viral infections has been less studied in fish.					
36405758	10	34	theme	zebrafish	1466:1474	arg1	larvae					1476:1481	zebrafish larvae	1466:1481	zebrafish larvae	1466:1481	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	0	35	theme	spring	99:104	arg1	viremia					106:112	spring viremia	99:112	spring viremia of carp virus infection	99:136	The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection.					
36405758	12	36	theme	type	1702:1705	arg1	IFN					1709:1711	type I IFN	1702:1711	type I IFN antiviral response in ZF4 cells	1702:1743	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	15	37	from	composition	2009:2019	arg1	zebrafish					2049:2057	zebrafish	2049:2057	zebrafish	2049:2057	Lastly, dietary β-glucan changed the composition of intestinal microbiota in zebrafish, with reduced abundance of Proteobacteria and an enrichment of Fusobacteria and Firmicutes.					
36405758	10	38	theme	β-glucan	1454:1461	arg1	effect					1444:1449	the antiviral effect	1430:1449	the antiviral effect of β-glucan in zebrafish larvae	1430:1481	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	12	39	theme	IFN	1709:1711	arg1	response					1723:1730	type I IFN antiviral response	1702:1730	type I IFN antiviral response in ZF4 cells	1702:1743	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	7	40	theme	rRNA	825:828	arg1	pyrosequencing					835:848	rRNA gene pyrosequencing	825:848	rRNA gene pyrosequencing	825:848	The intestinal microbiota was evaluated by 16S rRNA gene pyrosequencing.					
36405758	16	41	theme	I	2294:2294	arg1	IFN					2296:2298	type I IFN	2289:2298	type I IFN antiviral immune response of fish after viral infection	2289:2354	To sum up, our results indicate that the β-glucan enhanced resistance of zebrafish against SVCV and the mechanism involved stimulation of type I IFN antiviral immune response of fish after viral infection.					
36405758	3	42	theme	β-glucan	326:333	arg1	effects					315:321	the effects	311:321	the effects of β-glucan on the resistance of zebrafish against spring viraemia of carp virus (SVCV)	311:409	In this study, we investigated the effects of β-glucan on the resistance of zebrafish against spring viraemia of carp virus (SVCV) and elucidated the underlying mechanisms.					
36405758	8	43	theme	SVCV	999:1002	arg1	challenge					1004:1012	SVCV challenge	999:1012	SVCV challenge (P < 0.05)	999:1023	Results showed that dietary supplementation of 0.025% β-glucan significantly increased survival rate of zebrafish compared with control group after SVCV challenge (P < 0.05).					
36405758	8	43	theme	SVCV	999:1002	arg1	<					1017:1017	P < 0.05	1015:1022	P < 0.05	1015:1022	Results showed that dietary supplementation of 0.025% β-glucan significantly increased survival rate of zebrafish compared with control group after SVCV challenge (P < 0.05).					
36405758	16	44	theme	IFN	2296:2298	arg1	response					2317:2324	type I IFN antiviral immune response	2289:2324	type I IFN antiviral immune response of fish after viral infection	2289:2354	To sum up, our results indicate that the β-glucan enhanced resistance of zebrafish against SVCV and the mechanism involved stimulation of type I IFN antiviral immune response of fish after viral infection.					
36405758	3	45	theme	spring	374:379	arg1	viraemia					381:388	spring viraemia	374:388	spring viraemia of carp virus (SVCV)	374:409	In this study, we investigated the effects of β-glucan on the resistance of zebrafish against spring viraemia of carp virus (SVCV) and elucidated the underlying mechanisms.					
36405758	16	46	theme	immune	2310:2315	arg1	response					2317:2324	type I IFN antiviral immune response	2289:2324	type I IFN antiviral immune response of fish after viral infection	2289:2354	To sum up, our results indicate that the β-glucan enhanced resistance of zebrafish against SVCV and the mechanism involved stimulation of type I IFN antiviral immune response of fish after viral infection.					
36405758	16	47	theme	type	2289:2292	arg1	IFN					2296:2298	type I IFN	2289:2298	type I IFN antiviral immune response of fish after viral infection	2289:2354	To sum up, our results indicate that the β-glucan enhanced resistance of zebrafish against SVCV and the mechanism involved stimulation of type I IFN antiviral immune response of fish after viral infection.					
36405758	14	48	theme	ZF4	1961:1963	arg1	cells					1965:1969	ZF4 cells	1961:1969	ZF4 cells	1961:1969	Inhibition of autophagy blocked the antiviral effect of β-glucan in ZF4 cells.					
36405758	3	49	theme	underlying	430:439	arg1	mechanisms					441:450	the underlying mechanisms	426:450	the underlying mechanisms	426:450	In this study, we investigated the effects of β-glucan on the resistance of zebrafish against spring viraemia of carp virus (SVCV) and elucidated the underlying mechanisms.					
36405758	0	50	theme	yeast	39:43	arg1	effect					4:9	The effect	0:9	The effect	0:9	The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection.					
36405758	0	50	theme	yeast	39:43	arg1	mechanism					26:34	underlying mechanism	15:34	underlying mechanism of yeast	15:43	The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection.					
36405758	9	51	theme	IFN-stimulated	1238:1251	arg1	mxb					1260:1262	mxb	1260:1262	mxb	1260:1262	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	9	51	theme	IFN-stimulated	1238:1251	arg1	genes					1253:1257	IFN-stimulated genes	1238:1257	IFN-stimulated genes (mxb, mxc)	1238:1268	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	6	52	theme	autophagy	741:749	arg1	level					751:755	autophagy level	741:755	autophagy level post SVCV infection	741:775	We further investigated the effect of β-glucan on autophagy level post SVCV infection.					
36405758	9	53	dep	genes	1209:1213	arg1	ifnφ3					1230:1234	ifnφ3	1230:1234	ifnφ3	1230:1234	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	9	53	dep	genes	1209:1213	arg1	ifnφ2					1223:1227	ifnφ2	1223:1227	ifnφ2	1223:1227	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	9	53	dep	genes	1209:1213	arg1	ifnφ1					1216:1220	ifnφ1	1216:1220	ifnφ1	1216:1220	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	10	54	theme	antiviral	1518:1526	arg1	function					1528:1535	β-glucan-mediated antiviral function	1500:1535	β-glucan-mediated antiviral function	1500:1535	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	6	55	theme	β-glucan	729:736	arg1	effect					719:724	the effect	715:724	the effect of β-glucan on autophagy level post SVCV infection	715:775	We further investigated the effect of β-glucan on autophagy level post SVCV infection.					
36405758	12	56	theme	Myd88	1809:1813	arg1	independent					1794:1804	independent	1794:1804	independent	1794:1804	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	12	56	theme	Myd88	1809:1813	arg1	effect					1764:1769	the antiviral effect	1750:1769	the antiviral effect of β-glucan in ZF4	1750:1788	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	8	57	theme	dietary	871:877	arg1	supplementation					879:893	dietary supplementation	871:893	dietary supplementation of 0.025% β-glucan	871:912	Results showed that dietary supplementation of 0.025% β-glucan significantly increased survival rate of zebrafish compared with control group after SVCV challenge (P < 0.05).					
36405758	9	58	theme	genes	1085:1089	arg1	expression					1071:1080	the expression	1067:1080	the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7	1067:1373	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	16	59	theme	antiviral	2300:2308	arg1	response					2317:2324	type I IFN antiviral immune response	2289:2324	type I IFN antiviral immune response of fish after viral infection	2289:2354	To sum up, our results indicate that the β-glucan enhanced resistance of zebrafish against SVCV and the mechanism involved stimulation of type I IFN antiviral immune response of fish after viral infection.					
36405758	15	60	theme	Proteobacteria	2086:2099	arg1	enrichment					2108:2117	an enrichment	2105:2117	an enrichment of Fusobacteria and Firmicutes	2105:2148	Lastly, dietary β-glucan changed the composition of intestinal microbiota in zebrafish, with reduced abundance of Proteobacteria and an enrichment of Fusobacteria and Firmicutes.					
36405758	15	60	theme	Proteobacteria	2086:2099	arg1	abundance					2073:2081	reduced abundance	2065:2081	reduced abundance of Proteobacteria	2065:2099	Lastly, dietary β-glucan changed the composition of intestinal microbiota in zebrafish, with reduced abundance of Proteobacteria and an enrichment of Fusobacteria and Firmicutes.					
36405758	8	61	theme	%	903:903	arg1	β-glucan					905:912	0.025% β-glucan	898:912	0.025% β-glucan	898:912	Results showed that dietary supplementation of 0.025% β-glucan significantly increased survival rate of zebrafish compared with control group after SVCV challenge (P < 0.05).					
36405758	10	62	theme	Morpholino	1376:1385	arg1	knockdown					1387:1395	Morpholino knockdown	1376:1395	Morpholino knockdown of type I IFN receptors	1376:1419	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	10	63	theme	IFN	1573:1575	arg1	response					1584:1591	IFN immune response	1573:1591	IFN immune response	1573:1591	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	10	64	from	effect	1444:1449	arg1	larvae					1476:1481	zebrafish larvae	1466:1481	zebrafish larvae	1466:1481	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	15	65	theme	microbiota	2035:2044	arg1	composition					2009:2019	the composition	2005:2019	the composition of intestinal microbiota in zebrafish	2005:2057	Lastly, dietary β-glucan changed the composition of intestinal microbiota in zebrafish, with reduced abundance of Proteobacteria and an enrichment of Fusobacteria and Firmicutes.					
36405758	10	66	theme	receptors	1411:1419	arg1	knockdown					1387:1395	Morpholino knockdown	1376:1395	Morpholino knockdown of type I IFN receptors	1376:1419	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	9	67	theme	antiviral	1113:1121	arg1	pathway					1130:1136	type I IFN antiviral immune pathway	1102:1136	type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection	1102:1185	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	11	68	from	replication	1632:1642	arg1	cells					1659:1663	ZF4 cells	1655:1663	ZF4 cells	1655:1663	Furthermore, β-glucan can inhibit the replication of SVCV in ZF4 cells.					
36405758	8	69	theme	survival	938:945	arg1	rate					947:950	survival rate	938:950	survival rate of zebrafish	938:963	Results showed that dietary supplementation of 0.025% β-glucan significantly increased survival rate of zebrafish compared with control group after SVCV challenge (P < 0.05).					
36405758	10	70	theme	I	1405:1405	arg1	IFN					1407:1409	type I IFN	1400:1409	type I IFN receptors	1400:1419	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	0	71	theme	carp	117:120	arg1	infection					128:136	carp virus infection	117:136	carp virus infection	117:136	The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection.					
36405758	12	72	theme	β-glucan	1774:1781	arg1	independent					1794:1804	independent	1794:1804	independent	1794:1804	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	12	72	theme	β-glucan	1774:1781	arg1	effect					1764:1769	the antiviral effect	1750:1769	the antiviral effect of β-glucan in ZF4	1750:1788	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	0	73	theme	infection	128:136	arg1	viremia					106:112	spring viremia	99:112	spring viremia of carp virus infection	99:136	The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection.					
36405758	2	74	theme	yeast	214:218	arg1	β-glucan					220:227	yeast β-glucan	214:227	yeast β-glucan	214:227	However, the effect of yeast β-glucan on viral infections has been less studied in fish.					
36405758	9	75	theme	IFN	1310:1312	arg1	pathway					1324:1330	the IFN signaling pathway	1306:1330	the IFN signaling pathway	1306:1330	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	4	76	theme	control	479:485	arg1	diet					487:490	a control diet	477:490	a control diet	477:490	Zebrafish were fed with a control diet or diet supplemented with 0.01% and 0.025% β-glucan for 2 weeks, and were challenged by SVCV.					
36405758	11	77	theme	ZF4	1655:1657	arg1	cells					1659:1663	ZF4 cells	1655:1663	ZF4 cells	1655:1663	Furthermore, β-glucan can inhibit the replication of SVCV in ZF4 cells.					
36405758	5	78	theme	Zebrafish	586:594	arg1	cells					623:627	Zebrafish embryonic fibroblast (ZF4) cells	586:627	Zebrafish embryonic fibroblast (ZF4) cells	586:627	Zebrafish embryonic fibroblast (ZF4) cells were treated with 5 μg/mL β-glucan and were infected by SVCV.					
36405758	5	79	theme	ZF4	618:620	arg1	cells					623:627	Zebrafish embryonic fibroblast (ZF4) cells	586:627	Zebrafish embryonic fibroblast (ZF4) cells	586:627	Zebrafish embryonic fibroblast (ZF4) cells were treated with 5 μg/mL β-glucan and were infected by SVCV.					
36405758	9	80	from	pathway	1130:1136	arg1	spleen					1145:1150	the spleen	1141:1150	the spleen of zebrafish after viral infection	1141:1185	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	11	81	theme	SVCV	1647:1650	arg1	replication					1632:1642	the replication	1628:1642	the replication of SVCV in ZF4 cells	1628:1663	Furthermore, β-glucan can inhibit the replication of SVCV in ZF4 cells.					
36405758	5	82	theme	fibroblast	606:615	arg1	cells					623:627	Zebrafish embryonic fibroblast (ZF4) cells	586:627	Zebrafish embryonic fibroblast (ZF4) cells	586:627	Zebrafish embryonic fibroblast (ZF4) cells were treated with 5 μg/mL β-glucan and were infected by SVCV.					
36405758	0	83	theme	antiviral	57:65	arg1	resistance					67:76	antiviral resistance	57:76	antiviral resistance of zebrafish against spring viremia of carp virus infection	57:136	The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection.					
36405758	1	84	used	used	157:160	arg2	β-glucan					139:146	β-glucan	139:146	β-glucan	139:146	β-glucan has been used as immunostimulant for fish.					
36405758	1	84	used	used	157:160	arg2	immunostimulant					165:179	immunostimulant	165:179	immunostimulant for fish	165:188	β-glucan has been used as immunostimulant for fish.					
36405758	8	85	theme	zebrafish	955:963	arg1	rate					947:950	survival rate	938:950	survival rate of zebrafish	938:963	Results showed that dietary supplementation of 0.025% β-glucan significantly increased survival rate of zebrafish compared with control group after SVCV challenge (P < 0.05).					
36405758	13	86	theme	SVCV	1877:1880	arg1	infection					1882:1890	SVCV infection	1877:1890	SVCV infection	1877:1890	Interestingly, β-glucan induced autophagy in ZF4 cells after SVCV infection.					
36405758	14	87	theme	autophagy	1907:1915	arg1	Inhibition					1893:1902	Inhibition	1893:1902	Inhibition of autophagy	1893:1915	Inhibition of autophagy blocked the antiviral effect of β-glucan in ZF4 cells.					
36405758	12	88	theme	I	1707:1707	arg1	IFN					1709:1711	type I IFN	1702:1711	type I IFN antiviral response in ZF4 cells	1702:1743	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	3	89	theme	virus	398:402	arg1	viraemia					381:388	spring viraemia	374:388	spring viraemia of carp virus (SVCV)	374:409	In this study, we investigated the effects of β-glucan on the resistance of zebrafish against spring viraemia of carp virus (SVCV) and elucidated the underlying mechanisms.					
36405758	12	90	theme	antiviral	1713:1721	arg1	response					1723:1730	type I IFN antiviral response	1702:1730	type I IFN antiviral response in ZF4 cells	1702:1743	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	6	91	from	effect	719:724	arg1	level					751:755	autophagy level	741:755	autophagy level post SVCV infection	741:775	We further investigated the effect of β-glucan on autophagy level post SVCV infection.					
36405758	8	92	theme	control	979:985	arg1	group					987:991	control group	979:991	control group	979:991	Results showed that dietary supplementation of 0.025% β-glucan significantly increased survival rate of zebrafish compared with control group after SVCV challenge (P < 0.05).					
36405758	0	93	theme	underlying	15:24	arg1	mechanism					26:34	underlying mechanism	15:34	underlying mechanism of yeast	15:43	The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection.					
36405758	7	94	theme	gene	830:833	arg1	pyrosequencing					835:848	rRNA gene pyrosequencing	825:848	rRNA gene pyrosequencing	825:848	The intestinal microbiota was evaluated by 16S rRNA gene pyrosequencing.					
36405758	15	95	theme	reduced	2065:2071	arg1	abundance					2073:2081	reduced abundance	2065:2081	reduced abundance of Proteobacteria	2065:2099	Lastly, dietary β-glucan changed the composition of intestinal microbiota in zebrafish, with reduced abundance of Proteobacteria and an enrichment of Fusobacteria and Firmicutes.					
36405758	8	96	theme	P	1015:1015	arg1	challenge					1004:1012	SVCV challenge	999:1012	SVCV challenge (P < 0.05)	999:1023	Results showed that dietary supplementation of 0.025% β-glucan significantly increased survival rate of zebrafish compared with control group after SVCV challenge (P < 0.05).					
36405758	8	96	theme	P	1015:1015	arg1	<					1017:1017	P < 0.05	1015:1022	P < 0.05	1015:1022	Results showed that dietary supplementation of 0.025% β-glucan significantly increased survival rate of zebrafish compared with control group after SVCV challenge (P < 0.05).					
36405758	6	97	theme	SVCV	762:765	arg1	infection					767:775	SVCV infection	762:775	SVCV infection	762:775	We further investigated the effect of β-glucan on autophagy level post SVCV infection.					
36405758	3	98	theme	carp	393:396	arg1	SVCV					405:408	SVCV	405:408	SVCV	405:408	In this study, we investigated the effects of β-glucan on the resistance of zebrafish against spring viraemia of carp virus (SVCV) and elucidated the underlying mechanisms.					
36405758	3	98	theme	carp	393:396	arg1	virus					398:402	carp virus	393:402	carp virus (SVCV)	393:409	In this study, we investigated the effects of β-glucan on the resistance of zebrafish against spring viraemia of carp virus (SVCV) and elucidated the underlying mechanisms.					
36405758	14	99	theme	β-glucan	1949:1956	arg1	effect					1939:1944	the antiviral effect	1925:1944	the antiviral effect of β-glucan in ZF4 cells	1925:1969	Inhibition of autophagy blocked the antiviral effect of β-glucan in ZF4 cells.					
36405758	9	100	theme	Dietary	1026:1032	arg1	β-glucan					1034:1041	Dietary β-glucan	1026:1041	Dietary β-glucan	1026:1041	Dietary β-glucan significantly increased the expression of genes related to type I IFN antiviral immune pathway in the spleen of zebrafish after viral infection, including type I IFN genes (ifnφ1, ifnφ2, ifnφ3), IFN-stimulated genes (mxb, mxc), as well as other genes involved in the IFN signaling pathway, including tlr7, rig1, mavs, irf3 and irf7.					
36405758	5	101	theme	μg/mL	649:653	arg1	β-glucan					655:662	5 μg/mL β-glucan	647:662	5 μg/mL β-glucan	647:662	Zebrafish embryonic fibroblast (ZF4) cells were treated with 5 μg/mL β-glucan and were infected by SVCV.					
36405758	12	102	from	effect	1764:1769	arg1	ZF4					1786:1788	ZF4	1786:1788	ZF4	1786:1788	However, β-glucan did not stimulate type I IFN antiviral response in ZF4 cells, and the antiviral effect of β-glucan in ZF4 was independent of Myd88.					
36405758	3	103	theme	zebrafish	356:364	arg1	resistance					342:351	the resistance	338:351	the resistance of zebrafish against spring viraemia of carp virus (SVCV)	338:409	In this study, we investigated the effects of β-glucan on the resistance of zebrafish against spring viraemia of carp virus (SVCV) and elucidated the underlying mechanisms.					
36405758	16	104	theme	response	2317:2324	arg1	stimulation					2274:2284	stimulation	2274:2284	stimulation of type I IFN antiviral immune response of fish after viral infection	2274:2354	To sum up, our results indicate that the β-glucan enhanced resistance of zebrafish against SVCV and the mechanism involved stimulation of type I IFN antiviral immune response of fish after viral infection.					
36405758	4	105	theme	β-glucan	535:542	arg1	%					522:522	0.01%	518:522	0.01%	518:522	Zebrafish were fed with a control diet or diet supplemented with 0.01% and 0.025% β-glucan for 2 weeks, and were challenged by SVCV.					
36405758	10	106	theme	β-glucan-mediated	1500:1516	arg1	function					1528:1535	β-glucan-mediated antiviral function	1500:1535	β-glucan-mediated antiviral function	1500:1535	Morpholino knockdown of type I IFN receptors dampened the antiviral effect of β-glucan in zebrafish larvae, indicating that β-glucan-mediated antiviral function was at least partially dependent on IFN immune response.					
36405758	15	107	theme	Firmicutes	2139:2148	arg1	enrichment					2108:2117	an enrichment	2105:2117	an enrichment of Fusobacteria and Firmicutes	2105:2148	Lastly, dietary β-glucan changed the composition of intestinal microbiota in zebrafish, with reduced abundance of Proteobacteria and an enrichment of Fusobacteria and Firmicutes.					
36405758	15	107	theme	Firmicutes	2139:2148	arg1	abundance					2073:2081	reduced abundance	2065:2081	reduced abundance of Proteobacteria	2065:2099	Lastly, dietary β-glucan changed the composition of intestinal microbiota in zebrafish, with reduced abundance of Proteobacteria and an enrichment of Fusobacteria and Firmicutes.					
36405758	16	108	theme	fish	2329:2332	arg1	response					2317:2324	type I IFN antiviral immune response	2289:2324	type I IFN antiviral immune response of fish after viral infection	2289:2354	To sum up, our results indicate that the β-glucan enhanced resistance of zebrafish against SVCV and the mechanism involved stimulation of type I IFN antiviral immune response of fish after viral infection.					
36405758	7	109	theme	intestinal	782:791	arg1	microbiota					793:802	The intestinal microbiota	778:802	The intestinal microbiota	778:802	The intestinal microbiota was evaluated by 16S rRNA gene pyrosequencing.					
36405758	15	110	theme	Fusobacteria	2122:2133	arg1	enrichment					2108:2117	an enrichment	2105:2117	an enrichment of Fusobacteria and Firmicutes	2105:2148	Lastly, dietary β-glucan changed the composition of intestinal microbiota in zebrafish, with reduced abundance of Proteobacteria and an enrichment of Fusobacteria and Firmicutes.					
36405758	15	110	theme	Fusobacteria	2122:2133	arg1	abundance					2073:2081	reduced abundance	2065:2081	reduced abundance of Proteobacteria	2065:2099	Lastly, dietary β-glucan changed the composition of intestinal microbiota in zebrafish, with reduced abundance of Proteobacteria and an enrichment of Fusobacteria and Firmicutes.					
35697833	8	0	theme	treatment	1153:1161	arg1	process					1163:1169	treatment process	1153:1169	treatment process	1153:1169	The optimal decolorization was 94.7% at 1.5 g/l after 90 min of treatment process.					
35697833	11	1	theme	hazardous	1520:1528	arg1	pollutants					1536:1545	hazardous toxic pollutants	1520:1545	hazardous toxic pollutants	1520:1545	The sorption trials marked Fe3O4-NPs-based biopolymer as efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate.					
35697833	8	2	from	1.5 g/l	1129:1135	arg1	decolorization					1101:1114	The optimal decolorization	1089:1114	The optimal decolorization	1089:1114	The optimal decolorization was 94.7% at 1.5 g/l after 90 min of treatment process.					
35697833	8	2	from	1.5 g/l	1129:1135	arg1	%					1124:1124	94.7%	1120:1124	94.7%	1120:1124	The optimal decolorization was 94.7% at 1.5 g/l after 90 min of treatment process.					
35697833	0	3	theme	sustainable	76:86	arg1	remediation					88:98	sustainable remediation	76:98	sustainable remediation of wastewater contaminants	76:125	Aspergillus tamarii mediated green synthesis of magnetic chitosan beads for sustainable remediation of wastewater contaminants.					
35697833	4	4	theme	variables	543:551	arg1	effect					511:516	The effect	507:516	The effect of different independent variables on the Fe3O4-NPs production	507:579	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	11	5	theme	sustainable	1474:1484	arg1	biosorbent					1486:1495	efficient and sustainable biosorbent	1460:1495	efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate	1460:1580	The sorption trials marked Fe3O4-NPs-based biopolymer as efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate.					
35697833	0	6	theme	wastewater	103:112	arg1	contaminants					114:125	wastewater contaminants	103:125	wastewater contaminants	103:125	Aspergillus tamarii mediated green synthesis of magnetic chitosan beads for sustainable remediation of wastewater contaminants.					
35697833	4	7	theme	independent	531:541	arg1	variables					543:551	different independent variables	521:551	different independent variables	521:551	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	5	8	theme	optimum	697:703	arg1	production					715:724	The optimum Fe3O4-NPs production	693:724	The optimum Fe3O4-NPs production	693:724	The optimum Fe3O4-NPs production was determined using incubation period (24 h), temperature (30 °C), pH (12), stirring speed (100 rpm) and stirring time (1 h).					
35697833	7	9	theme	modified	957:964	arg1	nanocomposite					966:978	The modified nanocomposite	953:978	The modified nanocomposite	953:978	The modified nanocomposite exhibited remarkable removal capability with improved stability and regeneration, compared to control beads.					
35697833	2	10	from	potentiality	314:325	arg1	bioremediation					369:382	sustainable bioremediation	357:382	sustainable bioremediation of toxic substances from contaminated water	357:426	This study focuses on the potentiality of Fe3O4-NPs-based polymer in sustainable bioremediation of toxic substances from contaminated water.					
35697833	1	11	theme	recent	272:277	arg1	decades					279:285	recent decades	272:285	recent decades	272:285	The release of different hazardous substances into the water bodies during the industrial and textile processing stages is a serious problem in recent decades.					
35697833	8	12	theme	process	1163:1169	arg1	90 min					1143:1148	90 min	1143:1148	90 min of treatment process	1143:1169	The optimal decolorization was 94.7% at 1.5 g/l after 90 min of treatment process.					
35697833	6	13	theme	Fe3O4-NPs	874:882	arg1	incorporation					857:869	The incorporation	853:869	The incorporation of Fe3O4-NPs into chitosan beads	853:902	The incorporation of Fe3O4-NPs into chitosan beads was successfully performed using sol-gel method.					
35697833	5	14	theme	Fe3O4-NPs	705:713	arg1	production					715:724	The optimum Fe3O4-NPs production	693:724	The optimum Fe3O4-NPs production	693:724	The optimum Fe3O4-NPs production was determined using incubation period (24 h), temperature (30 °C), pH (12), stirring speed (100 rpm) and stirring time (1 h).					
35697833	9	15	theme	beads	1202:1206	arg1	reusability					1176:1186	The reusability	1172:1186	The reusability of biosorbent beads	1172:1206	The reusability of biosorbent beads displayed 75.35% decolorization after the 7th cycle.					
35697833	11	16	theme	Fe3O4-NPs-based	1430:1444	arg1	biopolymer					1446:1455	marked Fe3O4-NPs-based biopolymer	1423:1455	marked Fe3O4-NPs-based biopolymer	1423:1455	The sorption trials marked Fe3O4-NPs-based biopolymer as efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate.					
35697833	2	17	theme	Fe3O4-NPs-based	330:344	arg1	polymer					346:352	Fe3O4-NPs-based polymer	330:352	Fe3O4-NPs-based polymer	330:352	This study focuses on the potentiality of Fe3O4-NPs-based polymer in sustainable bioremediation of toxic substances from contaminated water.					
35697833	5	18	theme	stirring	832:839	arg1	1 h					847:849	1 h	847:849	1 h	847:849	The optimum Fe3O4-NPs production was determined using incubation period (24 h), temperature (30 °C), pH (12), stirring speed (100 rpm) and stirring time (1 h).					
35697833	5	18	theme	stirring	832:839	arg1	time					841:844	stirring time	832:844	stirring time (1 h)	832:850	The optimum Fe3O4-NPs production was determined using incubation period (24 h), temperature (30 °C), pH (12), stirring speed (100 rpm) and stirring time (1 h).					
35697833	10	19	theme	wastewater	1391:1400	arg1	PO4					1370:1372	PO4	1370:1372	PO4	1370:1372	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	19	theme	wastewater	1391:1400	arg1	parameters					1331:1340	physico-chemical parameters	1314:1340	physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater	1314:1400	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	19	theme	wastewater	1391:1400	arg1	pH					1343:1344	pH	1343:1344	pH	1343:1344	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	19	theme	wastewater	1391:1400	arg1	COD					1357:1359	COD	1357:1359	COD	1357:1359	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	19	theme	wastewater	1391:1400	arg1	EC					1362:1363	EC	1362:1363	EC	1362:1363	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	19	theme	wastewater	1391:1400	arg1	TDS					1347:1349	TDS	1347:1349	TDS	1347:1349	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	19	theme	wastewater	1391:1400	arg1	TSS					1352:1354	TSS	1352:1354	TSS	1352:1354	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	4	20	from	effect	511:516	arg1	production					570:579	the Fe3O4-NPs production	556:579	the Fe3O4-NPs production	556:579	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	3	21	theme	first	495:499	arg1	time					501:504	the first time	491:504	the first time	491:504	The biosynthesis of Fe3O4-NPs by A. tamarii was performed for the first time.					
35697833	11	22	theme	marked	1423:1428	arg1	biopolymer					1446:1455	marked Fe3O4-NPs-based biopolymer	1423:1455	marked Fe3O4-NPs-based biopolymer	1423:1455	The sorption trials marked Fe3O4-NPs-based biopolymer as efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate.					
35697833	1	23	theme	water	183:187	arg1	bodies					189:194	the water bodies	179:194	the water bodies during the industrial and textile processing stages	179:246	The release of different hazardous substances into the water bodies during the industrial and textile processing stages is a serious problem in recent decades.					
35697833	0	24	theme	contaminants	114:125	arg1	remediation					88:98	sustainable remediation	76:98	sustainable remediation of wastewater contaminants	76:125	Aspergillus tamarii mediated green synthesis of magnetic chitosan beads for sustainable remediation of wastewater contaminants.					
35697833	9	25	theme	%	1223:1223	arg1	decolorization					1225:1238	75.35% decolorization	1218:1238	75.35% decolorization	1218:1238	The reusability of biosorbent beads displayed 75.35% decolorization after the 7th cycle.					
35697833	9	26	theme	75.35	1218:1222	arg1	%					1223:1223	%	1223:1223	%	1223:1223	The reusability of biosorbent beads displayed 75.35% decolorization after the 7th cycle.					
35697833	10	27	theme	significant	1289:1299	arg1	reduction					1301:1309	a highly significant reduction	1280:1309	a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater	1280:1400	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	5	28	theme	stirring	803:810	arg1	speed					812:816	stirring speed	803:816	stirring speed (100 rpm)	803:826	The optimum Fe3O4-NPs production was determined using incubation period (24 h), temperature (30 °C), pH (12), stirring speed (100 rpm) and stirring time (1 h).					
35697833	5	28	theme	stirring	803:810	arg1	100 rpm					819:825	100 rpm	819:825	100 rpm	819:825	The optimum Fe3O4-NPs production was determined using incubation period (24 h), temperature (30 °C), pH (12), stirring speed (100 rpm) and stirring time (1 h).					
35697833	11	29	from	elimination	1505:1515	arg1	rate					1577:1580	a high-speed rate	1564:1580	a high-speed rate	1564:1580	The sorption trials marked Fe3O4-NPs-based biopolymer as efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate.					
35697833	2	30	from	bioremediation	369:382	arg1	water					422:426	contaminated water	409:426	contaminated water	409:426	This study focuses on the potentiality of Fe3O4-NPs-based polymer in sustainable bioremediation of toxic substances from contaminated water.					
35697833	6	31	theme	chitosan	889:896	arg1	beads					898:902	chitosan beads	889:902	chitosan beads	889:902	The incorporation of Fe3O4-NPs into chitosan beads was successfully performed using sol-gel method.					
35697833	2	32	theme	contaminated	409:420	arg1	water					422:426	contaminated water	409:426	contaminated water	409:426	This study focuses on the potentiality of Fe3O4-NPs-based polymer in sustainable bioremediation of toxic substances from contaminated water.					
35697833	0	33	theme	Aspergillus	0:10	arg1	tamarii					12:18	Aspergillus tamarii	0:18	Aspergillus tamarii	0:18	Aspergillus tamarii mediated green synthesis of magnetic chitosan beads for sustainable remediation of wastewater contaminants.					
35697833	10	34	theme	contaminated	1378:1389	arg1	wastewater					1391:1400	contaminated wastewater	1378:1400	contaminated wastewater	1378:1400	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	9	35	theme	7th	1250:1252	arg1	cycle					1254:1258	the 7th cycle	1246:1258	the 7th cycle	1246:1258	The reusability of biosorbent beads displayed 75.35% decolorization after the 7th cycle.					
35697833	0	36	theme	green	29:33	arg1	synthesis					35:43	green synthesis	29:43	green synthesis of magnetic chitosan beads for sustainable remediation of wastewater contaminants	29:125	Aspergillus tamarii mediated green synthesis of magnetic chitosan beads for sustainable remediation of wastewater contaminants.					
35697833	5	37	theme	incubation	747:756	arg1	period					758:763	incubation period	747:763	incubation period (24 h)	747:770	The optimum Fe3O4-NPs production was determined using incubation period (24 h), temperature (30 °C), pH (12), stirring speed (100 rpm) and stirring time (1 h).					
35697833	5	37	theme	incubation	747:756	arg1	24 h					766:769	24 h	766:769	24 h	766:769	The optimum Fe3O4-NPs production was determined using incubation period (24 h), temperature (30 °C), pH (12), stirring speed (100 rpm) and stirring time (1 h).					
35697833	11	38	theme	pollutants	1536:1545	arg1	elimination					1505:1515	the elimination	1501:1515	the elimination of hazardous toxic pollutants of wastewater in a high-speed rate	1501:1580	The sorption trials marked Fe3O4-NPs-based biopolymer as efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate.					
35697833	9	39	theme	biosorbent	1191:1200	arg1	beads					1202:1206	biosorbent beads	1191:1206	biosorbent beads	1191:1206	The reusability of biosorbent beads displayed 75.35% decolorization after the 7th cycle.					
35697833	11	40	theme	efficient	1460:1468	arg1	biosorbent					1486:1495	efficient and sustainable biosorbent	1460:1495	efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate	1460:1580	The sorption trials marked Fe3O4-NPs-based biopolymer as efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate.					
35697833	1	41	theme	industrial	207:216	arg1	stages					241:246	the industrial and textile processing stages	203:246	the industrial and textile processing stages	203:246	The release of different hazardous substances into the water bodies during the industrial and textile processing stages is a serious problem in recent decades.					
35697833	7	42	theme	removal	1001:1007	arg1	capability					1009:1018	remarkable removal capability	990:1018	remarkable removal capability	990:1018	The modified nanocomposite exhibited remarkable removal capability with improved stability and regeneration, compared to control beads.					
35697833	4	43	theme	Plackett-Burman	602:616	arg1	design					618:623	Plackett-Burman design	602:623	Plackett-Burman design	602:623	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	4	43	theme	Plackett-Burman	602:616	arg1	CCD					655:657	CCD	655:657	CCD	655:657	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	11	44	from	wastewater	1550:1559	arg1	rate					1577:1580	a high-speed rate	1564:1580	a high-speed rate	1564:1580	The sorption trials marked Fe3O4-NPs-based biopolymer as efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate.					
35697833	10	45	theme	parameters	1331:1340	arg1	reduction					1301:1309	a highly significant reduction	1280:1309	a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater	1280:1400	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	1	46	theme	hazardous	153:161	arg1	substances					163:172	different hazardous substances	143:172	different hazardous substances	143:172	The release of different hazardous substances into the water bodies during the industrial and textile processing stages is a serious problem in recent decades.					
35697833	1	47	from	problem	261:267	arg1	decades					279:285	recent decades	272:285	recent decades	272:285	The release of different hazardous substances into the water bodies during the industrial and textile processing stages is a serious problem in recent decades.					
35697833	4	48	theme	Methodology	680:690	arg1	design					618:623	Plackett-Burman design	602:623	Plackett-Burman design	602:623	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	4	48	theme	Methodology	680:690	arg1	CCD					655:657	CCD	655:657	CCD	655:657	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	4	48	theme	Methodology	680:690	arg1	design					647:652	central composite design	629:652	central composite design	629:652	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	10	49	theme	physico-chemical	1314:1329	arg1	PO4					1370:1372	PO4	1370:1372	PO4	1370:1372	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	49	theme	physico-chemical	1314:1329	arg1	parameters					1331:1340	physico-chemical parameters	1314:1340	physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater	1314:1400	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	49	theme	physico-chemical	1314:1329	arg1	pH					1343:1344	pH	1343:1344	pH	1343:1344	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	49	theme	physico-chemical	1314:1329	arg1	COD					1357:1359	COD	1357:1359	COD	1357:1359	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	49	theme	physico-chemical	1314:1329	arg1	EC					1362:1363	EC	1362:1363	EC	1362:1363	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	49	theme	physico-chemical	1314:1329	arg1	TDS					1347:1349	TDS	1347:1349	TDS	1347:1349	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	49	theme	physico-chemical	1314:1329	arg1	TSS					1352:1354	TSS	1352:1354	TSS	1352:1354	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	1	50	theme	textile	222:228	arg1	stages					241:246	the industrial and textile processing stages	203:246	the industrial and textile processing stages	203:246	The release of different hazardous substances into the water bodies during the industrial and textile processing stages is a serious problem in recent decades.					
35697833	3	51	theme	Fe3O4-NPs	449:457	arg1	biosynthesis					433:444	The biosynthesis	429:444	The biosynthesis of Fe3O4-NPs by A. tamarii	429:471	The biosynthesis of Fe3O4-NPs by A. tamarii was performed for the first time.					
35697833	6	52	theme	sol-gel	937:943	arg1	method					945:950	sol-gel method	937:950	sol-gel method	937:950	The incorporation of Fe3O4-NPs into chitosan beads was successfully performed using sol-gel method.					
35697833	7	53	theme	control	1074:1080	arg1	beads					1082:1086	control beads	1074:1086	control beads	1074:1086	The modified nanocomposite exhibited remarkable removal capability with improved stability and regeneration, compared to control beads.					
35697833	7	54	theme	improved	1025:1032	arg1	stability					1034:1042	improved stability	1025:1042	improved stability	1025:1042	The modified nanocomposite exhibited remarkable removal capability with improved stability and regeneration, compared to control beads.					
35697833	1	55	theme	processing	230:239	arg1	stages					241:246	the industrial and textile processing stages	203:246	the industrial and textile processing stages	203:246	The release of different hazardous substances into the water bodies during the industrial and textile processing stages is a serious problem in recent decades.					
35697833	0	56	theme	chitosan	57:64	arg1	beads					66:70	magnetic chitosan beads	48:70	magnetic chitosan beads	48:70	Aspergillus tamarii mediated green synthesis of magnetic chitosan beads for sustainable remediation of wastewater contaminants.					
35697833	2	57	theme	sustainable	357:367	arg1	bioremediation					369:382	sustainable bioremediation	357:382	sustainable bioremediation of toxic substances from contaminated water	357:426	This study focuses on the potentiality of Fe3O4-NPs-based polymer in sustainable bioremediation of toxic substances from contaminated water.					
35697833	0	58	theme	magnetic	48:55	arg1	beads					66:70	magnetic chitosan beads	48:70	magnetic chitosan beads	48:70	Aspergillus tamarii mediated green synthesis of magnetic chitosan beads for sustainable remediation of wastewater contaminants.					
35697833	8	59	theme	optimal	1093:1099	arg1	decolorization					1101:1114	The optimal decolorization	1089:1114	The optimal decolorization	1089:1114	The optimal decolorization was 94.7% at 1.5 g/l after 90 min of treatment process.					
35697833	8	59	theme	optimal	1093:1099	arg1	%					1124:1124	94.7%	1120:1124	94.7%	1120:1124	The optimal decolorization was 94.7% at 1.5 g/l after 90 min of treatment process.					
35697833	4	60	theme	composite	637:645	arg1	design					647:652	central composite design	629:652	central composite design	629:652	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	1	61	theme	substances	163:172	arg1	release					132:138	The release	128:138	The release of different hazardous substances into the water bodies during the industrial and textile processing stages	128:246	The release of different hazardous substances into the water bodies during the industrial and textile processing stages is a serious problem in recent decades.					
35697833	1	61	theme	substances	163:172	arg1	problem					261:267	a serious problem	251:267	a serious problem in recent decades	251:285	The release of different hazardous substances into the water bodies during the industrial and textile processing stages is a serious problem in recent decades.					
35697833	4	62	theme	Fe3O4-NPs	560:568	arg1	production					570:579	the Fe3O4-NPs production	556:579	the Fe3O4-NPs production	556:579	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	11	63	theme	high-speed	1566:1575	arg1	rate					1577:1580	a high-speed rate	1564:1580	a high-speed rate	1564:1580	The sorption trials marked Fe3O4-NPs-based biopolymer as efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate.					
35697833	11	64	theme	toxic	1530:1534	arg1	pollutants					1536:1545	hazardous toxic pollutants	1520:1545	hazardous toxic pollutants	1520:1545	The sorption trials marked Fe3O4-NPs-based biopolymer as efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate.					
35697833	2	65	from	water	422:426	arg1	bioremediation					369:382	sustainable bioremediation	357:382	sustainable bioremediation of toxic substances from contaminated water	357:426	This study focuses on the potentiality of Fe3O4-NPs-based polymer in sustainable bioremediation of toxic substances from contaminated water.					
35697833	2	65	from	water	422:426	arg1	substances					393:402	toxic substances	387:402	toxic substances from contaminated water	387:426	This study focuses on the potentiality of Fe3O4-NPs-based polymer in sustainable bioremediation of toxic substances from contaminated water.					
35697833	2	66	theme	polymer	346:352	arg1	potentiality					314:325	the potentiality	310:325	the potentiality of Fe3O4-NPs-based polymer in sustainable bioremediation of toxic substances from contaminated water	310:426	This study focuses on the potentiality of Fe3O4-NPs-based polymer in sustainable bioremediation of toxic substances from contaminated water.					
35697833	11	67	from	rate	1577:1580	arg1	elimination					1505:1515	the elimination	1501:1515	the elimination of hazardous toxic pollutants of wastewater in a high-speed rate	1501:1580	The sorption trials marked Fe3O4-NPs-based biopolymer as efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate.					
35697833	10	68	dep	parameters	1331:1340	arg1	PO4					1370:1372	PO4	1370:1372	PO4	1370:1372	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	68	dep	parameters	1331:1340	arg1	parameters					1331:1340	physico-chemical parameters	1314:1340	physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater	1314:1400	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	68	dep	parameters	1331:1340	arg1	pH					1343:1344	pH	1343:1344	pH	1343:1344	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	68	dep	parameters	1331:1340	arg1	COD					1357:1359	COD	1357:1359	COD	1357:1359	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	68	dep	parameters	1331:1340	arg1	EC					1362:1363	EC	1362:1363	EC	1362:1363	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	68	dep	parameters	1331:1340	arg1	TDS					1347:1349	TDS	1347:1349	TDS	1347:1349	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	10	68	dep	parameters	1331:1340	arg1	TSS					1352:1354	TSS	1352:1354	TSS	1352:1354	The results showed a highly significant reduction of physico-chemical parameters (pH, TDS, TSS, COD, EC, and PO4) of contaminated wastewater.					
35697833	7	69	theme	remarkable	990:999	arg1	capability					1009:1018	remarkable removal capability	990:1018	remarkable removal capability	990:1018	The modified nanocomposite exhibited remarkable removal capability with improved stability and regeneration, compared to control beads.					
35697833	1	70	theme	different	143:151	arg1	substances					163:172	different hazardous substances	143:172	different hazardous substances	143:172	The release of different hazardous substances into the water bodies during the industrial and textile processing stages is a serious problem in recent decades.					
35697833	4	71	theme	Surface	672:678	arg1	Methodology					680:690	Response Surface Methodology	663:690	Response Surface Methodology	663:690	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	4	72	theme	Response	663:670	arg1	Methodology					680:690	Response Surface Methodology	663:690	Response Surface Methodology	663:690	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	2	73	theme	substances	393:402	arg1	bioremediation					369:382	sustainable bioremediation	357:382	sustainable bioremediation of toxic substances from contaminated water	357:426	This study focuses on the potentiality of Fe3O4-NPs-based polymer in sustainable bioremediation of toxic substances from contaminated water.					
35697833	11	74	theme	wastewater	1550:1559	arg1	elimination					1505:1515	the elimination	1501:1515	the elimination of hazardous toxic pollutants of wastewater in a high-speed rate	1501:1580	The sorption trials marked Fe3O4-NPs-based biopolymer as efficient and sustainable biosorbent for the elimination of hazardous toxic pollutants of wastewater in a high-speed rate.					
35697833	4	75	theme	central	629:635	arg1	design					647:652	central composite design	629:652	central composite design	629:652	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	0	76	theme	beads	66:70	arg1	synthesis					35:43	green synthesis	29:43	green synthesis of magnetic chitosan beads for sustainable remediation of wastewater contaminants	29:125	Aspergillus tamarii mediated green synthesis of magnetic chitosan beads for sustainable remediation of wastewater contaminants.					
35697833	4	77	theme	different	521:529	arg1	variables					543:551	different independent variables	521:551	different independent variables	521:551	The effect of different independent variables on the Fe3O4-NPs production were optimized using Plackett-Burman design and central composite design (CCD) of Response Surface Methodology.					
35697833	2	78	theme	toxic	387:391	arg1	substances					393:402	toxic substances	387:402	toxic substances from contaminated water	387:426	This study focuses on the potentiality of Fe3O4-NPs-based polymer in sustainable bioremediation of toxic substances from contaminated water.					
35697833	1	79	theme	serious	253:259	arg1	release					132:138	The release	128:138	The release of different hazardous substances into the water bodies during the industrial and textile processing stages	128:246	The release of different hazardous substances into the water bodies during the industrial and textile processing stages is a serious problem in recent decades.					
35697833	1	79	theme	serious	253:259	arg1	problem					261:267	a serious problem	251:267	a serious problem in recent decades	251:285	The release of different hazardous substances into the water bodies during the industrial and textile processing stages is a serious problem in recent decades.					
36283551	2	0	theme	primary	508:514	arg1	evaluation					413:422	evaluation	413:422	evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs	413:497	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	2	0	theme	primary	508:514	arg1	Degradation					346:356	Degradation	346:356	Degradation of the potentially harmful methylene blue (MB) dye	346:407	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	2	0	theme	primary	508:514	arg1	objectives					516:525	the primary objectives	504:525	the primary objectives of this study	504:539	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	5	1	theme	E.	1233:1234	arg1	pathogens					1241:1249	E. coli pathogens	1233:1249	E. coli pathogens	1233:1249	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs explored effective degradation and antibacterial results as 89.39 % in neutral medium and 7.85 mm against E. coli pathogens, respectively.					
36283551	1	2	theme	0.02 wt	223:229	arg1	%					230:230	0.02 wt%	223:230	0.02 wt%	223:230	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	0	3	theme	dye	75:77	arg1	degrader					79:86	dye degrader	75:86	dye degrader	75:86	Chitosan and carbon nitride doped barium hydroxide nanoparticles served as dye degrader and bactericidal potential: A molecular docking study.					
36283551	2	4	dep	blue	395:398	arg1	MB					401:402	MB	401:402	MB	401:402	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	5	5	theme	neutral	1198:1204	arg1	medium					1206:1211	neutral medium	1198:1211	neutral medium	1198:1211	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs explored effective degradation and antibacterial results as 89.39 % in neutral medium and 7.85 mm against E. coli pathogens, respectively.					
36283551	4	6	dep	coli	906:909	arg1	coli					915:918	E. coli	912:918	E. coli	912:918	The photocatalytic activity was assessed against the degradation of MB by varying pH, whereas Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens were utilized to determine bactericidal potential in terms of inhibition zone (mm) measured through Vernier caliper.					
36283551	3	7	theme	surface	741:747	arg1	morphology					749:758	surface morphology	741:758	surface morphology	741:758	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	7	theme	surface	741:747	arg1	features					663:670	optical features	655:670	optical features	655:670	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	0	8	theme	bactericidal	92:103	arg1	potential					105:113	bactericidal potential	92:113	bactericidal potential	92:113	Chitosan and carbon nitride doped barium hydroxide nanoparticles served as dye degrader and bactericidal potential: A molecular docking study.					
36283551	4	9	used	utilized	974:981	arg2	pathogens					959:967	Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens	894:967	Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens	894:967	The photocatalytic activity was assessed against the degradation of MB by varying pH, whereas Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens were utilized to determine bactericidal potential in terms of inhibition zone (mm) measured through Vernier caliper.					
36283551	1	10	dep	Chitosan	143:150	arg1	NPs					294:296	NPs	294:296	NPs	294:296	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	1	10	dep	Chitosan	143:150	arg1	nanoparticles					279:291	doped barium hydroxide (Ba(OH)2) nanoparticles	246:291	doped barium hydroxide (Ba(OH)2) nanoparticles (NPs)	246:297	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	3	11	theme	groups	724:729	arg1	presence					731:738	functional groups presence	713:738	functional groups presence	713:738	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	11	theme	groups	724:729	arg1	features					663:670	optical features	655:670	optical features	655:670	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	2	12	theme	potential	445:453	arg1	evaluation					413:422	evaluation	413:422	evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs	413:497	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	2	12	theme	potential	445:453	arg1	Degradation					346:356	Degradation	346:356	Degradation of the potentially harmful methylene blue (MB) dye	346:407	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	2	12	theme	potential	445:453	arg1	objectives					516:525	the primary objectives	504:525	the primary objectives of this study	504:539	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	3	13	theme	morphological	618:630	arg1	techniques					632:641	structural, optical and morphological techniques	594:641	structural, optical and morphological techniques	594:641	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	2	14	theme	NPs	495:497	arg1	potential					445:453	the antibacterial potential	427:453	the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs	427:497	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	4	15	theme	coli	906:909	arg1	pathogens					959:967	Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens	894:967	Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens	894:967	The photocatalytic activity was assessed against the degradation of MB by varying pH, whereas Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens were utilized to determine bactericidal potential in terms of inhibition zone (mm) measured through Vernier caliper.					
36283551	1	16	theme	0.04 wt	236:242	arg1	%					243:243	0.04 wt%	236:243	0.04 wt%	236:243	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	2	17	theme	antibacterial	431:443	arg1	potential					445:453	the antibacterial potential	427:453	the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs	427:497	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	6	18	theme	rationale	1395:1403	arg1	mechanism					1405:1413	rationale mechanism	1395:1413	rationale mechanism governing the anti-bacterial potential of these synthesized NPs	1395:1477	In silico, molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus were performed to rationale mechanism governing the anti-bacterial potential of these synthesized NPs.					
36283551	2	19	theme	study	535:539	arg1	evaluation					413:422	evaluation	413:422	evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs	413:497	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	2	19	theme	study	535:539	arg1	Degradation					346:356	Degradation	346:356	Degradation of the potentially harmful methylene blue (MB) dye	346:407	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	2	19	theme	study	535:539	arg1	objectives					516:525	the primary objectives	504:525	the primary objectives of this study	504:539	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	6	20	from	coli	1358:1361	arg1	gyrase					1315:1320	DNA gyrase	1311:1320	DNA gyrase	1311:1320	In silico, molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus were performed to rationale mechanism governing the anti-bacterial potential of these synthesized NPs.					
36283551	6	20	from	coli	1358:1361	arg1	enzyme					1338:1343	β-lactamase enzyme	1326:1343	β-lactamase enzyme	1326:1343	In silico, molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus were performed to rationale mechanism governing the anti-bacterial potential of these synthesized NPs.					
36283551	2	21	theme	blue	395:398	arg1	dye					405:407	the potentially harmful methylene blue (MB) dye	361:407	the potentially harmful methylene blue (MB) dye	361:407	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	3	22	theme	optical	606:612	arg1	techniques					632:641	structural, optical and morphological techniques	594:641	structural, optical and morphological techniques	594:641	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	2	23	theme	harmful	377:383	arg1	dye					405:407	the potentially harmful methylene blue (MB) dye	361:407	the potentially harmful methylene blue (MB) dye	361:407	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	4	24	theme	zone	1042:1045	arg1	terms					1022:1026	terms	1022:1026	terms of inhibition zone (mm) measured through Vernier caliper	1022:1083	The photocatalytic activity was assessed against the degradation of MB by varying pH, whereas Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens were utilized to determine bactericidal potential in terms of inhibition zone (mm) measured through Vernier caliper.					
36283551	2	25	theme	dye	405:407	arg1	evaluation					413:422	evaluation	413:422	evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs	413:497	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	2	25	theme	dye	405:407	arg1	Degradation					346:356	Degradation	346:356	Degradation of the potentially harmful methylene blue (MB) dye	346:407	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	2	25	theme	dye	405:407	arg1	objectives					516:525	the primary objectives	504:525	the primary objectives of this study	504:539	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	4	26	theme	inhibition	1031:1040	arg1	mm					1048:1049	mm	1048:1049	mm	1048:1049	The photocatalytic activity was assessed against the degradation of MB by varying pH, whereas Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens were utilized to determine bactericidal potential in terms of inhibition zone (mm) measured through Vernier caliper.					
36283551	4	26	theme	inhibition	1031:1040	arg1	zone					1042:1045	inhibition zone	1031:1045	inhibition zone (mm) measured through Vernier caliper	1031:1083	The photocatalytic activity was assessed against the degradation of MB by varying pH, whereas Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens were utilized to determine bactericidal potential in terms of inhibition zone (mm) measured through Vernier caliper.					
36283551	1	27	theme	different	161:169	arg1	concentration					171:183	different concentration	161:183	different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%)	161:244	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	1	27	theme	different	161:169	arg1	g-C3N4					214:219	g-C3N4	214:219	g-C3N4	214:219	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	0	28	theme	Chitosan	0:7	arg1	nanoparticles					51:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles	0:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles	0:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles served as dye degrader and bactericidal potential: A molecular docking study.					
36283551	3	29	theme	optical	655:661	arg1	morphology					749:758	surface morphology	741:758	surface morphology	741:758	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	29	theme	optical	655:661	arg1	crystallinity					761:773	crystallinity	761:773	crystallinity	761:773	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	29	theme	optical	655:661	arg1	formation					679:687	phase formation	673:687	phase formation	673:687	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	29	theme	optical	655:661	arg1	presence					731:738	functional groups presence	713:738	functional groups presence	713:738	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	29	theme	optical	655:661	arg1	features					663:670	optical features	655:670	optical features	655:670	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	29	theme	optical	655:661	arg1	spacing					791:797	interlayer spacing	780:797	interlayer spacing	780:797	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	29	theme	optical	655:661	arg1	composition					700:710	elemental composition	690:710	elemental composition	690:710	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	30	theme	produced	559:566	arg1	NPs					568:570	the produced NPs	555:570	the produced NPs	555:570	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	4	31	dep	aureus	940:945	arg1	aureus					951:956	S. aureus	948:956	S. aureus	948:956	The photocatalytic activity was assessed against the degradation of MB by varying pH, whereas Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens were utilized to determine bactericidal potential in terms of inhibition zone (mm) measured through Vernier caliper.					
36283551	4	32	theme	bactericidal	996:1007	arg1	potential					1009:1017	bactericidal potential	996:1017	bactericidal potential	996:1017	The photocatalytic activity was assessed against the degradation of MB by varying pH, whereas Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens were utilized to determine bactericidal potential in terms of inhibition zone (mm) measured through Vernier caliper.					
36283551	5	33	from	7.85 mm	1217:1223	arg1	medium					1206:1211	neutral medium	1198:1211	neutral medium	1198:1211	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs explored effective degradation and antibacterial results as 89.39 % in neutral medium and 7.85 mm against E. coli pathogens, respectively.					
36283551	0	34	theme	nitride	20:26	arg1	nanoparticles					51:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles	0:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles	0:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles served as dye degrader and bactericidal potential: A molecular docking study.					
36283551	0	35	theme	molecular	118:126	arg1	study					136:140	A molecular docking study	116:140	A molecular docking study	116:140	Chitosan and carbon nitride doped barium hydroxide nanoparticles served as dye degrader and bactericidal potential: A molecular docking study.					
36283551	6	36	theme	NPs	1475:1477	arg1	potential					1444:1452	the anti-bacterial potential	1425:1452	the anti-bacterial potential of these synthesized NPs	1425:1477	In silico, molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus were performed to rationale mechanism governing the anti-bacterial potential of these synthesized NPs.					
36283551	6	37	from	aureus	1370:1375	arg1	gyrase					1315:1320	DNA gyrase	1311:1320	DNA gyrase	1311:1320	In silico, molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus were performed to rationale mechanism governing the anti-bacterial potential of these synthesized NPs.					
36283551	6	37	from	aureus	1370:1375	arg1	enzyme					1338:1343	β-lactamase enzyme	1326:1343	β-lactamase enzyme	1326:1343	In silico, molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus were performed to rationale mechanism governing the anti-bacterial potential of these synthesized NPs.					
36283551	5	38	dep	E.	1233:1234	arg1	coli					1236:1239	coli	1236:1239	coli	1236:1239	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs explored effective degradation and antibacterial results as 89.39 % in neutral medium and 7.85 mm against E. coli pathogens, respectively.					
36283551	0	39	theme	carbon	13:18	arg1	nitride					20:26	carbon nitride	13:26	carbon nitride	13:26	Chitosan and carbon nitride doped barium hydroxide nanoparticles served as dye degrader and bactericidal potential: A molecular docking study.					
36283551	1	40	theme	doped	246:250	arg1	NPs					294:296	NPs	294:296	NPs	294:296	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	1	40	theme	doped	246:250	arg1	nanoparticles					279:291	doped barium hydroxide (Ba(OH)2) nanoparticles	246:291	doped barium hydroxide (Ba(OH)2) nanoparticles (NPs)	246:297	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	2	41	theme	CS/g-C3N4-doped	471:485	arg1	OH					490:491	OH	490:491	OH	490:491	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	2	41	theme	CS/g-C3N4-doped	471:485	arg1	Ba					487:488	CS/g-C3N4-doped Ba	471:488	the produced CS/g-C3N4-doped Ba(OH)2 NPs	458:497	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	0	42	dep	served	65:70	arg1	study					136:140	A molecular docking study	116:140	A molecular docking study	116:140	Chitosan and carbon nitride doped barium hydroxide nanoparticles served as dye degrader and bactericidal potential: A molecular docking study.					
36283551	1	43	theme	graphitic	188:196	arg1	nitride					205:211	graphitic carbon nitride	188:211	graphitic carbon nitride	188:211	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	0	44	theme	barium	34:39	arg1	nanoparticles					51:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles	0:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles	0:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles served as dye degrader and bactericidal potential: A molecular docking study.					
36283551	1	45	theme	barium	252:257	arg1	Ba					270:271	Ba(OH)2	270:276	Ba(OH)2	270:276	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	1	45	theme	barium	252:257	arg1	hydroxide					259:267	barium hydroxide	252:267	doped barium hydroxide (Ba(OH)2) nanoparticles (NPs)	246:297	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	2	46	theme	produced	462:469	arg1	NPs					495:497	the produced CS/g-C3N4-doped Ba(OH)2 NPs	458:497	the produced CS/g-C3N4-doped Ba(OH)2 NPs	458:497	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	4	47	theme	aureus	940:945	arg1	pathogens					959:967	Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens	894:967	Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens	894:967	The photocatalytic activity was assessed against the degradation of MB by varying pH, whereas Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens were utilized to determine bactericidal potential in terms of inhibition zone (mm) measured through Vernier caliper.					
36283551	1	48	theme	carbon	198:203	arg1	nitride					205:211	graphitic carbon nitride	188:211	graphitic carbon nitride	188:211	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	0	49	theme	doped	28:32	arg1	nanoparticles					51:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles	0:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles	0:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles served as dye degrader and bactericidal potential: A molecular docking study.					
36283551	1	50	theme	hydroxide	259:267	arg1	NPs					294:296	NPs	294:296	NPs	294:296	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	1	50	theme	hydroxide	259:267	arg1	nanoparticles					279:291	doped barium hydroxide (Ba(OH)2) nanoparticles	246:291	doped barium hydroxide (Ba(OH)2) nanoparticles (NPs)	246:297	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	0	51	theme	docking	128:134	arg1	study					136:140	A molecular docking study	116:140	A molecular docking study	116:140	Chitosan and carbon nitride doped barium hydroxide nanoparticles served as dye degrader and bactericidal potential: A molecular docking study.					
36283551	5	52	theme	antibacterial	1162:1174	arg1	results					1176:1182	effective degradation and antibacterial results	1136:1182	results	1176:1182	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs explored effective degradation and antibacterial results as 89.39 % in neutral medium and 7.85 mm against E. coli pathogens, respectively.					
36283551	1	53	dep	concentration	171:183	arg1	%					230:230	0.02 wt%	223:230	0.02 wt%	223:230	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	1	53	dep	concentration	171:183	arg1	%					243:243	0.04 wt%	236:243	0.04 wt%	236:243	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	4	54	theme	Vernier	1069:1075	arg1	caliper					1077:1083	Vernier caliper	1069:1083	Vernier caliper	1069:1083	The photocatalytic activity was assessed against the degradation of MB by varying pH, whereas Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens were utilized to determine bactericidal potential in terms of inhibition zone (mm) measured through Vernier caliper.					
36283551	1	55	theme	nitride	205:211	arg1	Chitosan					143:150	Chitosan	143:150	Chitosan (CS)	143:155	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	1	55	theme	nitride	205:211	arg1	concentration					171:183	different concentration	161:183	different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%)	161:244	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	1	55	theme	nitride	205:211	arg1	g-C3N4					214:219	g-C3N4	214:219	g-C3N4	214:219	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	1	55	theme	nitride	205:211	arg1	CS					153:154	CS	153:154	CS	153:154	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	4	56	theme	MB	868:869	arg1	degradation					853:863	the degradation	849:863	the degradation of MB	849:869	The photocatalytic activity was assessed against the degradation of MB by varying pH, whereas Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens were utilized to determine bactericidal potential in terms of inhibition zone (mm) measured through Vernier caliper.					
36283551	4	57	theme	photocatalytic	804:817	arg1	activity					819:826	The photocatalytic activity	800:826	The photocatalytic activity	800:826	The photocatalytic activity was assessed against the degradation of MB by varying pH, whereas Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens were utilized to determine bactericidal potential in terms of inhibition zone (mm) measured through Vernier caliper.					
36283551	1	58	theme	co-precipitation	322:337	arg1	route					339:343	co-precipitation route	322:343	co-precipitation route	322:343	Chitosan (CS) and different concentration of graphitic carbon nitride (g-C3N4) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)2) nanoparticles (NPs) were harvested through co-precipitation route.					
36283551	0	59	theme	hydroxide	41:49	arg1	nanoparticles					51:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles	0:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles	0:63	Chitosan and carbon nitride doped barium hydroxide nanoparticles served as dye degrader and bactericidal potential: A molecular docking study.					
36283551	2	60	theme	Ba	487:488	arg1	NPs					495:497	the produced CS/g-C3N4-doped Ba(OH)2 NPs	458:497	the produced CS/g-C3N4-doped Ba(OH)2 NPs	458:497	Degradation of the potentially harmful methylene blue (MB) dye and evaluation of the antibacterial potential of the produced CS/g-C3N4-doped Ba(OH)2 NPs were the primary objectives of this study.					
36283551	5	61	dep	CS/g-C3N4-doped	1099:1113	arg1	%					1096:1096	4 %	1094:1096	4 %	1094:1096	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs explored effective degradation and antibacterial results as 89.39 % in neutral medium and 7.85 mm against E. coli pathogens, respectively.					
36283551	6	62	theme	DNA	1311:1313	arg1	gyrase					1315:1320	DNA gyrase	1311:1320	DNA gyrase	1311:1320	In silico, molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus were performed to rationale mechanism governing the anti-bacterial potential of these synthesized NPs.					
36283551	3	63	theme	phase	673:677	arg1	features					663:670	optical features	655:670	optical features	655:670	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	63	theme	phase	673:677	arg1	formation					679:687	phase formation	673:687	phase formation	673:687	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	6	64	theme	molecular	1277:1285	arg1	studies					1295:1301	molecular docking studies	1277:1301	molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus	1277:1375	In silico, molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus were performed to rationale mechanism governing the anti-bacterial potential of these synthesized NPs.					
36283551	5	65	theme	effective	1136:1144	arg1	degradation					1146:1156	effective degradation and antibacterial results	1136:1182	degradation	1146:1156	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs explored effective degradation and antibacterial results as 89.39 % in neutral medium and 7.85 mm against E. coli pathogens, respectively.					
36283551	6	66	theme	docking	1287:1293	arg1	studies					1295:1301	molecular docking studies	1277:1301	molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus	1277:1375	In silico, molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus were performed to rationale mechanism governing the anti-bacterial potential of these synthesized NPs.					
36283551	6	67	theme	synthesized	1463:1473	arg1	NPs					1475:1477	these synthesized NPs	1457:1477	these synthesized NPs	1457:1477	In silico, molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus were performed to rationale mechanism governing the anti-bacterial potential of these synthesized NPs.					
36283551	6	68	theme	anti-bacterial	1429:1442	arg1	potential					1444:1452	the anti-bacterial potential	1425:1452	the anti-bacterial potential of these synthesized NPs	1425:1477	In silico, molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus were performed to rationale mechanism governing the anti-bacterial potential of these synthesized NPs.					
36283551	3	69	theme	functional	713:722	arg1	presence					731:738	functional groups presence	713:738	functional groups presence	713:738	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	69	theme	functional	713:722	arg1	features					663:670	optical features	655:670	optical features	655:670	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	70	theme	interlayer	780:789	arg1	features					663:670	optical features	655:670	optical features	655:670	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	70	theme	interlayer	780:789	arg1	spacing					791:797	interlayer spacing	780:797	interlayer spacing	780:797	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	5	71	from	%	1193:1193	arg1	medium					1206:1211	neutral medium	1198:1211	neutral medium	1198:1211	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs explored effective degradation and antibacterial results as 89.39 % in neutral medium and 7.85 mm against E. coli pathogens, respectively.					
36283551	6	72	theme	β-lactamase	1326:1336	arg1	enzyme					1338:1343	β-lactamase enzyme	1326:1343	β-lactamase enzyme	1326:1343	In silico, molecular docking studies against DNA gyrase and β-lactamase enzyme from both E. coli and S. aureus were performed to rationale mechanism governing the anti-bacterial potential of these synthesized NPs.					
36283551	3	73	theme	elemental	690:698	arg1	features					663:670	optical features	655:670	optical features	655:670	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	73	theme	elemental	690:698	arg1	composition					700:710	elemental composition	690:710	elemental composition	690:710	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	3	74	theme	structural	594:603	arg1	techniques					632:641	structural, optical and morphological techniques	594:641	structural, optical and morphological techniques	594:641	In addition, the produced NPs were analyzed through structural, optical and morphological techniques to evaluate optical features, phase formation, elemental composition, functional groups presence, surface morphology, crystallinity, and interlayer spacing.					
36283551	5	75	theme	CS/g-C3N4-doped	1099:1113	arg1	NPs					1123:1125	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs	1086:1125	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs	1086:1125	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs explored effective degradation and antibacterial results as 89.39 % in neutral medium and 7.85 mm against E. coli pathogens, respectively.					
36283551	5	76	theme	Ba	1115:1116	arg1	NPs					1123:1125	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs	1086:1125	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs	1086:1125	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs explored effective degradation and antibacterial results as 89.39 % in neutral medium and 7.85 mm against E. coli pathogens, respectively.					
36283551	5	77	theme	OH	1118:1119	arg1	NPs					1123:1125	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs	1086:1125	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs	1086:1125	Highly (4 %) CS/g-C3N4-doped Ba(OH)2 NPs explored effective degradation and antibacterial results as 89.39 % in neutral medium and 7.85 mm against E. coli pathogens, respectively.					
35908106	0	0	theme	fermentation	78:89	arg1	strategy					91:98	an efficient fermentation strategy	65:98	an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production	65:164	Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production.					
35908106	1	1	theme	Bacillus	309:316	arg1	bacteria					327:334	Bacillus subtilis bacteria	309:334	Bacillus subtilis bacteria	309:334	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	4	2	theme	nutritional	820:830	arg1	results					851:857	The nutritional and abiotic factor results	816:857	The nutritional and abiotic factor results	816:857	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	2	3	from	levels	455:460	arg1	bioreactor					510:519	a 5 L bioreactor	504:519	a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources	504:605	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	6	4	theme	silent	1364:1369	arg1	clusters					1376:1383	silent gene clusters	1364:1383	silent gene clusters	1364:1383	More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria.					
35908106	2	5	from	bioreactor	510:519	arg1	%					719:719	14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%	669:719	14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%	669:719	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	5	from	bioreactor	510:519	arg1	levels					455:460	The maximum levels	443:460	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes	443:662	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	0	6	theme	efficient	68:76	arg1	strategy					91:98	an efficient fermentation strategy	65:98	an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production	65:164	Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production.					
35908106	5	7	theme	GLA	1055:1057	arg1	higher					1113:1118	higher	1113:1118	higher	1113:1118	Interestingly, the GLA concentration of cocultures (20.0 ± 0.5%) was twofold higher than that of monocultures (8.27 ± 0.11%).					
35908106	5	7	theme	GLA	1055:1057	arg1	concentration					1059:1071	the GLA concentration	1051:1071	the GLA concentration of cocultures (20.0 ± 0.5%)	1051:1099	Interestingly, the GLA concentration of cocultures (20.0 ± 0.5%) was twofold higher than that of monocultures (8.27 ± 0.11%).					
35908106	4	8	dep	%	950:950	arg1	v/v					953:955	v/v	953:955	v/v	953:955	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	7	9	theme	prospective	1617:1627	arg1	biosynthesis					1629:1640	prospective biosynthesis	1617:1640	prospective biosynthesis of new products	1617:1656	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35908106	6	10	from	monocultures	1325:1336	arg1	absent					1315:1320	absent	1315:1320	absent	1315:1320	More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria.					
35908106	6	11	theme	decanoic	1278:1285	arg1	acid					1287:1290	decanoic acid	1278:1290	decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria	1278:1415	More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria.					
35908106	6	11	theme	decanoic	1278:1285	arg1	%					1304:1304	5.32 ± 0.20%	1293:1304	5.32 ± 0.20%	1293:1304	More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria.					
35908106	2	12	theme	ammonium	547:554	arg1	sulfate					556:562	ammonium sulfate	547:562	ammonium sulfate	547:562	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	12	theme	ammonium	547:554	arg1	carbon					579:584	the optimal carbon and nitrogen sources	567:605	carbon	579:584	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	12	theme	ammonium	547:554	arg1	sources					599:605	the optimal carbon and nitrogen sources	567:605	sources	599:605	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	12	theme	ammonium	547:554	arg1	cellobiose					532:541	cellobiose	532:541	cellobiose	532:541	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	1	13	theme	batch	423:427	arg1	fermentation					429:440	batch fermentation	423:440	batch fermentation	423:440	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	4	14	theme	agitation	988:996	arg1	speed					998:1002	200 rpm agitation speed	980:1002	200 rpm agitation speed	980:1002	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	4	15	theme	30 °C	929:933	arg1	temperature					935:945	30 °C temperature	929:945	30 °C temperature	929:945	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	6	16	with	cocultivation	1389:1401	arg1	bacteria					1408:1415	bacteria	1408:1415	bacteria	1408:1415	More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria.					
35908106	7	17	theme	lipids	1606:1611	arg1	production					1573:1582	the production	1569:1582	the production of GLA-rich microbial lipids and prospective biosynthesis of new products	1569:1656	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35908106	6	18	theme	additional	1245:1254	arg1	peak					1256:1259	one additional peak	1241:1259	one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria	1241:1415	More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria.					
35908106	0	19	theme	fungal	111:116	arg1	lipid					118:122	fungal lipid	111:122	fungal lipid	111:122	Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production.					
35908106	2	20	theme	nitrogen	590:597	arg1	sulfate					556:562	ammonium sulfate	547:562	ammonium sulfate	547:562	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	20	theme	nitrogen	590:597	arg1	sources					599:605	the optimal carbon and nitrogen sources	567:605	sources	599:605	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	20	theme	nitrogen	590:597	arg1	cellobiose					532:541	cellobiose	532:541	cellobiose	532:541	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	7	21	theme	products	1649:1656	arg1	lipids					1606:1611	GLA-rich microbial lipids	1587:1611	GLA-rich microbial lipids	1587:1611	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35908106	7	21	theme	products	1649:1656	arg1	biosynthesis					1629:1640	prospective biosynthesis	1617:1640	prospective biosynthesis of new products	1617:1656	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35908106	2	22	theme	fatty	481:485	arg1	acid					487:490	fatty acid	481:490	fatty acid	481:490	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	23	theme	biomass	465:471	arg1	%					719:719	14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%	669:719	14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%	669:719	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	23	theme	biomass	465:471	arg1	levels					455:460	The maximum levels	443:460	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes	443:662	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	4	24	theme	10	948:949	arg1	%					950:950	%	950:950	%	950:950	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	7	25	with	strategy	1523:1530	arg1	potential					1555:1563	industrialisation potential	1537:1563	industrialisation potential	1537:1563	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35908106	2	26	from	GLA	497:499	arg1	bioreactor					510:519	a 5 L bioreactor	504:519	a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources	504:605	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	7	27	theme	GLA-rich	1587:1594	arg1	lipids					1606:1611	GLA-rich microbial lipids	1587:1611	GLA-rich microbial lipids	1587:1611	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35908106	2	28	theme	acid	487:490	arg1	%					719:719	14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%	669:719	14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%	669:719	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	28	theme	acid	487:490	arg1	levels					455:460	The maximum levels	443:460	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes	443:662	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	1	29	dep	Bacillus	309:316	arg1	subtilis					318:325	subtilis	318:325	subtilis	318:325	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	2	30	theme	optimal	571:577	arg1	sulfate					556:562	ammonium sulfate	547:562	ammonium sulfate	547:562	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	30	theme	optimal	571:577	arg1	carbon					579:584	the optimal carbon and nitrogen sources	567:605	carbon	579:584	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	30	theme	optimal	571:577	arg1	cellobiose					532:541	cellobiose	532:541	cellobiose	532:541	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	7	31	theme	industrialisation	1537:1553	arg1	potential					1555:1563	industrialisation potential	1537:1563	industrialisation potential	1537:1563	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35908106	7	32	theme	microbial	1596:1604	arg1	lipids					1606:1611	GLA-rich microbial lipids	1587:1611	GLA-rich microbial lipids	1587:1611	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35908106	1	33	theme	oleaginous	249:258	arg1	fungus					260:265	an oleaginous fungus	246:265	an oleaginous fungus	246:265	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	1	33	theme	oleaginous	249:258	arg1	plumbeus					274:281	Mucor plumbeus	268:281	Mucor plumbeus	268:281	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	2	34	from	biomass	465:471	arg1	bioreactor					510:519	a 5 L bioreactor	504:519	a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources	504:605	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	1	35	theme	fungus	260:265	arg1	production					232:241	lipid and gamma-linolenic acid (GLA) production	195:241	lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus,	195:282	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	4	36	theme	highest	876:882	arg1	efficiency					895:904	the highest production efficiency	872:904	the highest production efficiency	872:904	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	4	37	theme	incubation	1017:1026	arg1	period					1028:1033	a 5-day incubation period	1009:1033	a 5-day incubation period	1009:1033	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	0	38	theme	Mucor	15:19	arg1	plumbeus					21:28	Mucor plumbeus	15:28	Mucor plumbeus	15:28	Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production.					
35908106	4	39	theme	production	884:893	arg1	efficiency					895:904	the highest production efficiency	872:904	the highest production efficiency	872:904	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	2	40	contain	containing	521:530	arg2	cellobiose					532:541	cellobiose	532:541	cellobiose	532:541	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	40	contain	containing	521:530	arg1	bioreactor					510:519	a 5 L bioreactor	504:519	a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources	504:605	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	40	contain	containing	521:530	arg2	carbon					579:584	the optimal carbon and nitrogen sources	567:605	carbon	579:584	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	40	contain	containing	521:530	arg2	sources					599:605	the optimal carbon and nitrogen sources	567:605	sources	599:605	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	40	contain	containing	521:530	arg2	sulfate					556:562	ammonium sulfate	547:562	ammonium sulfate	547:562	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	0	41	theme	Bacillus	34:41	arg1	bacterium					52:60	Bacillus subtilis bacterium	34:60	Bacillus subtilis bacterium	34:60	Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production.					
35908106	1	42	theme	Mucor	268:272	arg1	fungus					260:265	an oleaginous fungus	246:265	an oleaginous fungus	246:265	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	1	42	theme	Mucor	268:272	arg1	plumbeus					274:281	Mucor plumbeus	268:281	Mucor plumbeus	268:281	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	0	43	theme	acid	144:147	arg1	production					155:164	gamma-linolenic acid (GLA) production	128:164	gamma-linolenic acid (GLA) production	128:164	Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production.					
35908106	1	44	theme	nutritional	370:380	arg1	conditions					390:399	the environmental and nutritional culture conditions	348:399	conditions	390:399	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	6	45	theme	clusters	1376:1383	arg1	activation					1350:1359	activation	1350:1359	activation of silent gene clusters	1350:1383	More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria.					
35908106	1	46	theme	lipid	195:199	arg1	production					232:241	lipid and gamma-linolenic acid (GLA) production	195:241	lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus,	195:282	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	4	47	theme	%	950:950	arg1	composition					967:977	10% (v/v) inoculum composition	948:977	10% (v/v) inoculum composition	948:977	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	0	48	theme	gamma-linolenic	128:142	arg1	GLA					150:152	GLA	150:152	GLA	150:152	Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production.					
35908106	0	48	theme	gamma-linolenic	128:142	arg1	acid					144:147	gamma-linolenic acid	128:147	gamma-linolenic acid (GLA) production	128:164	Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production.					
35908106	1	49	theme	culture	382:388	arg1	conditions					390:399	the environmental and nutritional culture conditions	348:399	conditions	390:399	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	6	50	from	absent	1315:1320	arg1	monocultures					1325:1336	monocultures	1325:1336	monocultures	1325:1336	More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria.					
35908106	6	51	theme	peak	1256:1259	arg1	presence					1229:1236	the presence	1225:1236	the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria	1225:1415	More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria.					
35908106	2	52	theme	maximum	447:453	arg1	%					719:719	14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%	669:719	14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%	669:719	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	52	theme	maximum	447:453	arg1	levels					455:460	The maximum levels	443:460	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes	443:662	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	5	53	theme	cocultures	1076:1085	arg1	higher					1113:1118	higher	1113:1118	higher	1113:1118	Interestingly, the GLA concentration of cocultures (20.0 ± 0.5%) was twofold higher than that of monocultures (8.27 ± 0.11%).					
35908106	5	53	theme	cocultures	1076:1085	arg1	concentration					1059:1071	the GLA concentration	1051:1071	the GLA concentration of cocultures (20.0 ± 0.5%)	1051:1099	Interestingly, the GLA concentration of cocultures (20.0 ± 0.5%) was twofold higher than that of monocultures (8.27 ± 0.11%).					
35908106	2	54	from	lipid	474:478	arg1	bioreactor					510:519	a 5 L bioreactor	504:519	a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources	504:605	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	0	55	dep	Bacillus	34:41	arg1	subtilis					43:50	subtilis	43:50	subtilis	43:50	Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production.					
35908106	6	56	theme	cocultures	1204:1213	arg1	chromatograms					1187:1199	the GC chromatograms	1180:1199	the GC chromatograms of cocultures	1180:1213	More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria.					
35908106	2	57	theme	GLA	497:499	arg1	%					719:719	14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%	669:719	14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%	669:719	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	57	theme	GLA	497:499	arg1	levels					455:460	The maximum levels	443:460	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes	443:662	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	4	58	theme	factor	844:849	arg1	results					851:857	The nutritional and abiotic factor results	816:857	The nutritional and abiotic factor results	816:857	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	2	59	from	acid	487:490	arg1	bioreactor					510:519	a 5 L bioreactor	504:519	a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources	504:605	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	4	60	theme	abiotic	836:842	arg1	results					851:857	The nutritional and abiotic factor results	816:857	The nutritional and abiotic factor results	816:857	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	6	61	theme	GC	1184:1185	arg1	chromatograms					1187:1199	the GC chromatograms	1180:1199	the GC chromatograms of cocultures	1180:1213	More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria.					
35908106	2	62	theme	coculturing	642:652	arg1	processes					654:662	the coculturing processes	638:662	the coculturing processes	638:662	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	7	63	theme	new	1645:1647	arg1	products					1649:1656	new products	1645:1656	new products	1645:1656	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35908106	1	64	theme	gamma-linolenic	205:219	arg1	GLA					227:229	GLA	227:229	GLA	227:229	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	1	64	theme	gamma-linolenic	205:219	arg1	acid					221:224	gamma-linolenic acid	205:224	gamma-linolenic acid (GLA)	205:230	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	2	65	theme	L	508:508	arg1	bioreactor					510:519	a 5 L bioreactor	504:519	a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources	504:605	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	5	66	dep	higher	1113:1118	arg1	%					1158:1158	8.27 ± 0.11%	1147:1158	8.27 ± 0.11%	1147:1158	Interestingly, the GLA concentration of cocultures (20.0 ± 0.5%) was twofold higher than that of monocultures (8.27 ± 0.11%).					
35908106	2	67	theme	24 ± 0.8	697:704	arg1	14.5 ± 0.4 g/L					669:682	14.5 ± 0.4 g/L	669:682	14.5 ± 0.4 g/L	669:682	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	4	68	theme	5-day	1011:1015	arg1	period					1028:1033	a 5-day incubation period	1009:1033	a 5-day incubation period	1009:1033	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	1	69	theme	acid	221:224	arg1	production					232:241	lipid and gamma-linolenic acid (GLA) production	195:241	lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus,	195:282	This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation.					
35908106	7	70	theme	biosynthesis	1629:1640	arg1	production					1573:1582	the production	1569:1582	the production of GLA-rich microbial lipids and prospective biosynthesis of new products	1569:1656	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35908106	6	71	theme	gene	1371:1374	arg1	clusters					1376:1383	silent gene clusters	1364:1383	silent gene clusters	1364:1383	More importantly, the GC chromatograms of cocultures indicated the presence of one additional peak corresponding to decanoic acid (5.32 ± 0.20%) that is absent in monocultures, indicating activation of silent gene clusters via cocultivation with bacteria.					
35908106	3	72	theme	production	798:807	arg1	costs					809:813	production costs	798:813	production costs	798:813	This strategy uses cellobiose in place of glucose, decreasing production costs.					
35908106	7	73	theme	Mucor	1470:1474	arg1	plumbeus					1476:1483	Mucor plumbeus	1470:1483	Mucor plumbeus	1470:1483	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35908106	2	74	theme	lipid	474:478	arg1	%					719:719	14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%	669:719	14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%	669:719	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	2	74	theme	lipid	474:478	arg1	levels					455:460	The maximum levels	443:460	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes	443:662	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	4	75	theme	inoculum	958:965	arg1	composition					967:977	10% (v/v) inoculum composition	948:977	10% (v/v) inoculum composition	948:977	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	2	76	theme	41.5 ± 1.3	685:694	arg1	14.5 ± 0.4 g/L					669:682	14.5 ± 0.4 g/L	669:682	14.5 ± 0.4 g/L	669:682	The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/L, 41.5 ± 1.3, 24 ± 0.8, and 20 ± 0.5%, respectively.					
35908106	4	77	theme	200 rpm	980:986	arg1	speed					998:1002	200 rpm agitation speed	980:1002	200 rpm agitation speed	980:1002	The nutritional and abiotic factor results suggest that the highest production efficiency is achieved at 6.5 pH, 30 °C temperature, 10% (v/v) inoculum composition, 200 rpm agitation speed, and a 5-day incubation period.					
35908106	7	78	theme	promising	1513:1521	arg1	coculturing					1455:1465	coculturing	1455:1465	coculturing	1455:1465	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35908106	7	78	theme	promising	1513:1521	arg1	strategy					1523:1530	a promising strategy	1511:1530	a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products	1511:1656	This study is the first to show that coculturing of Mucor plumbeus with Bacillus subtilis is a promising strategy with industrialisation potential for the production of GLA-rich microbial lipids and prospective biosynthesis of new products.					
35322921	5	0	theme	inclusion	1015:1023	arg1	complexation					1025:1036	inclusion complexation	1015:1036	inclusion complexation	1015:1036	Two retention models were employed individually for evaluation of inclusion complexation between five racemates and sulfobutylether-β-cyclodextrin.					
35322921	2	1	theme	column	539:544	arg1	temperature					546:556	column temperature	539:556	column temperature	539:556	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	1	2	theme	N-alkyl	180:186	arg1	hydrochloride					244:256	venlafaxine hydrochloride	232:256	venlafaxine hydrochloride	232:256	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	2	theme	N-alkyl	180:186	arg1	hydrochloride					206:218	fluoxetine hydrochloride	195:218	fluoxetine hydrochloride	195:218	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	2	theme	N-alkyl	180:186	arg1	trans-paroxol					259:271	trans-paroxol	259:271	trans-paroxol	259:271	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	2	theme	N-alkyl	180:186	arg1	labetalol					221:229	labetalol	221:229	labetalol	221:229	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	2	theme	N-alkyl	180:186	arg1	drugs					188:192	five N-alkyl drugs	175:192	five N-alkyl drugs	175:192	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	2	theme	N-alkyl	180:186	arg1	sulfate					287:293	atropine sulfate	278:293	atropine sulfate	278:293	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	3	theme	atropine	278:285	arg1	drugs					188:192	five N-alkyl drugs	175:192	five N-alkyl drugs	175:192	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	3	theme	atropine	278:285	arg1	sulfate					287:293	atropine sulfate	278:293	atropine sulfate	278:293	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	4	theme	chiral	409:414	arg1	phase					423:427	chiral mobile phase additive	409:436	chiral mobile phase additive	409:436	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	4	theme	chiral	409:414	arg1	enantioseparations					153:170	Analytical enantioseparations	142:170	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate,	142:294	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	5	with	chromatography	355:368	arg1	sulfobutylether-β-cyclodextrin					375:404	sulfobutylether-β-cyclodextrin	375:404	sulfobutylether-β-cyclodextrin	375:404	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	3	6	theme	formation	617:625	arg1	constant					627:634	Apparent formation constant	608:634	Apparent formation constant between methanol, acetonitrile, and sulfobutylether-β-cyclodextrin	608:701	Apparent formation constant between methanol, acetonitrile, and sulfobutylether-β-cyclodextrin were determined to be 2.90 × 10-3 and 1.00 × 10-4 L mmol-1 under 25°C using UV-spectrophotometry.					
35322921	1	7	theme	mobile	416:421	arg1	phase					423:427	chiral mobile phase additive	409:436	chiral mobile phase additive	409:436	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	7	theme	mobile	416:421	arg1	enantioseparations					153:170	Analytical enantioseparations	142:170	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate,	142:294	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	6	8	with	model	1108:1112	arg1	adsorption					1127:1136	complex adsorption	1119:1136	complex adsorption	1119:1136	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	7	9	theme	stoichiometric	1405:1418	arg1	ratio					1420:1424	stoichiometric ratio	1405:1424	stoichiometric ratio for both of inclusion complex	1405:1454	In the selected mobile phase, stoichiometric ratio for both of inclusion complex was found to be 1:1.					
35322921	6	10	theme	trans-paroxol	1339:1351	arg1	behaviors					1326:1334	the retention behaviors	1312:1334	the retention behaviors of trans-paroxol and atropine sulfate	1312:1372	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	6	11	theme	first	1275:1279	arg1	model					1281:1285	the first model	1271:1285	the first model	1271:1285	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	6	11	theme	first	1275:1279	arg1	consistent					1296:1305	consistent	1296:1305	consistent	1296:1305	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	0	12	theme	mobile	119:124	arg1	phase					126:130	a chiral mobile phase additive	110:139	a chiral mobile phase additive	110:139	Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive.					
35322921	0	12	theme	mobile	119:124	arg1	sulfobutylether-β-cyclodextrin					76:105	sulfobutylether-β-cyclodextrin	76:105	sulfobutylether-β-cyclodextrin	76:105	Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive.					
35322921	7	13	theme	inclusion	1438:1446	arg1	complex					1448:1454	inclusion complex	1438:1454	inclusion complex	1438:1454	In the selected mobile phase, stoichiometric ratio for both of inclusion complex was found to be 1:1.					
35322921	6	14	theme	hydrochloride	1238:1250	arg1	enantiomers					1252:1262	venlafaxine hydrochloride enantiomers	1226:1262	venlafaxine hydrochloride enantiomers	1226:1262	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	1	15	theme	drugs	188:192	arg1	phase					423:427	chiral mobile phase additive	409:436	chiral mobile phase additive	409:436	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	15	theme	drugs	188:192	arg1	enantioseparations					153:170	Analytical enantioseparations	142:170	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate,	142:294	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	0	16	theme	chiral	112:117	arg1	phase					126:130	a chiral mobile phase additive	110:139	a chiral mobile phase additive	110:139	Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive.					
35322921	0	16	theme	chiral	112:117	arg1	sulfobutylether-β-cyclodextrin					76:105	sulfobutylether-β-cyclodextrin	76:105	sulfobutylether-β-cyclodextrin	76:105	Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive.					
35322921	1	17	theme	additive	429:436	arg1	phase					423:427	chiral mobile phase additive	409:436	chiral mobile phase additive	409:436	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	17	theme	additive	429:436	arg1	enantioseparations					153:170	Analytical enantioseparations	142:170	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate,	142:294	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	3	18	theme	Apparent	608:615	arg1	constant					627:634	Apparent formation constant	608:634	Apparent formation constant between methanol, acetonitrile, and sulfobutylether-β-cyclodextrin	608:701	Apparent formation constant between methanol, acetonitrile, and sulfobutylether-β-cyclodextrin were determined to be 2.90 × 10-3 and 1.00 × 10-4 L mmol-1 under 25°C using UV-spectrophotometry.					
35322921	6	19	theme	enantiomers	1252:1262	arg1	behavior					1173:1180	the retention behavior	1159:1180	the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers	1159:1262	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	2	20	theme	various	450:456	arg1	concentration					503:515	concentration	503:515	concentration of cyclodextrins	503:532	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	2	20	theme	various	450:456	arg1	factors					458:464	various factors	450:464	various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature	450:556	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	2	20	theme	various	450:456	arg1	composition					474:484	composition	474:484	composition of mobile phase	474:500	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	2	20	theme	various	450:456	arg1	temperature					546:556	column temperature	539:556	column temperature	539:556	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	7	21	theme	selected	1382:1389	arg1	phase					1398:1402	the selected mobile phase	1378:1402	the selected mobile phase	1378:1402	In the selected mobile phase, stoichiometric ratio for both of inclusion complex was found to be 1:1.					
35322921	6	22	theme	retention	1316:1324	arg1	behaviors					1326:1334	the retention behaviors	1312:1334	the retention behaviors of trans-paroxol and atropine sulfate	1312:1372	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	1	23	theme	fluoxetine	195:204	arg1	drugs					188:192	five N-alkyl drugs	175:192	five N-alkyl drugs	175:192	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	23	theme	fluoxetine	195:204	arg1	hydrochloride					206:218	fluoxetine hydrochloride	195:218	fluoxetine hydrochloride	195:218	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	4	24	theme	enantiomers-stationary	872:893	arg1	interaction					901:911	enantiomers-stationary phase interaction	872:911	enantiomers-stationary phase interaction	872:911	Van't Hoff plots were used to investigate thermodynamic parameters for enantiomers-stationary phase interaction and formation of inclusion complex.					
35322921	2	25	theme	factors	458:464	arg1	Effects					439:445	Effects	439:445	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity	439:592	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	0	26	theme	racemic	26:32	arg1	drugs					42:46	five racemic N-alkyl drugs	21:46	five racemic N-alkyl drugs	21:46	Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive.					
35322921	6	27	theme	complex	1119:1125	arg1	adsorption					1127:1136	complex adsorption	1119:1136	complex adsorption	1119:1136	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	0	28	theme	additive	132:139	arg1	phase					126:130	a chiral mobile phase additive	110:139	a chiral mobile phase additive	110:139	Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive.					
35322921	0	28	theme	additive	132:139	arg1	sulfobutylether-β-cyclodextrin					76:105	sulfobutylether-β-cyclodextrin	76:105	sulfobutylether-β-cyclodextrin	76:105	Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive.					
35322921	6	29	theme	labetalol	1211:1219	arg1	behavior					1173:1180	the retention behavior	1159:1180	the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers	1159:1262	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	2	30	theme	mobile	489:494	arg1	phase					496:500	mobile phase	489:500	mobile phase	489:500	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	5	31	theme	complexation	1025:1036	arg1	evaluation					1001:1010	evaluation	1001:1010	evaluation of inclusion complexation between five racemates and sulfobutylether-β-cyclodextrin	1001:1094	Two retention models were employed individually for evaluation of inclusion complexation between five racemates and sulfobutylether-β-cyclodextrin.					
35322921	2	32	theme	cyclodextrins	520:532	arg1	concentration					503:515	concentration	503:515	concentration of cyclodextrins	503:532	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	2	32	theme	cyclodextrins	520:532	arg1	composition					474:484	composition	474:484	composition of mobile phase	474:500	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	2	32	theme	cyclodextrins	520:532	arg1	temperature					546:556	column temperature	539:556	column temperature	539:556	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	0	33	theme	drugs	42:46	arg1	Enantioseparation					0:16	Enantioseparation	0:16	Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive.	0:140	Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive.					
35322921	1	34	theme	reverse	317:323	arg1	chromatography					355:368	reverse phase high-performance liquid chromatography	317:368	reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin	317:404	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	6	35	theme	hydrochloride	1196:1208	arg1	behavior					1173:1180	the retention behavior	1159:1180	the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers	1159:1262	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	5	36	theme	retention	953:961	arg1	models					963:968	Two retention models	949:968	Two retention models	949:968	Two retention models were employed individually for evaluation of inclusion complexation between five racemates and sulfobutylether-β-cyclodextrin.					
35322921	0	37	theme	N-alkyl	34:40	arg1	drugs					42:46	five racemic N-alkyl drugs	21:46	five racemic N-alkyl drugs	21:46	Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive.					
35322921	1	38	theme	phase	325:329	arg1	chromatography					355:368	reverse phase high-performance liquid chromatography	317:368	reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin	317:404	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	7	39	located	found	1460:1464	arg2	ratio					1420:1424	stoichiometric ratio	1405:1424	stoichiometric ratio for both of inclusion complex	1405:1454	In the selected mobile phase, stoichiometric ratio for both of inclusion complex was found to be 1:1.					
35322921	7	39	located	found	1460:1464	arg1	phase					1398:1402	the selected mobile phase	1378:1402	the selected mobile phase	1378:1402	In the selected mobile phase, stoichiometric ratio for both of inclusion complex was found to be 1:1.					
35322921	2	40	theme	phase	496:500	arg1	concentration					503:515	concentration	503:515	concentration of cyclodextrins	503:532	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	2	40	theme	phase	496:500	arg1	composition					474:484	composition	474:484	composition of mobile phase	474:500	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	2	40	theme	phase	496:500	arg1	temperature					546:556	column temperature	539:556	column temperature	539:556	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	1	41	theme	venlafaxine	232:242	arg1	hydrochloride					244:256	venlafaxine hydrochloride	232:256	venlafaxine hydrochloride	232:256	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	41	theme	venlafaxine	232:242	arg1	drugs					188:192	five N-alkyl drugs	175:192	five N-alkyl drugs	175:192	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	0	42	theme	reverse	51:57	arg1	HPLC					65:68	reverse phase HPLC	51:68	reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive	51:139	Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive.					
35322921	1	43	theme	high-performance	331:346	arg1	chromatography					355:368	reverse phase high-performance liquid chromatography	317:368	reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin	317:404	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	4	44	theme	complex	940:946	arg1	interaction					901:911	enantiomers-stationary phase interaction	872:911	enantiomers-stationary phase interaction	872:911	Van't Hoff plots were used to investigate thermodynamic parameters for enantiomers-stationary phase interaction and formation of inclusion complex.					
35322921	4	44	theme	complex	940:946	arg1	formation					917:925	formation	917:925	formation of inclusion complex	917:946	Van't Hoff plots were used to investigate thermodynamic parameters for enantiomers-stationary phase interaction and formation of inclusion complex.					
35322921	2	45	from	Effects	439:445	arg1	enantioselectivity					575:592	enantioselectivity	575:592	enantioselectivity	575:592	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	2	45	from	Effects	439:445	arg1	retention					561:569	retention	561:569	retention	561:569	Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied.					
35322921	4	46	theme	Va	801:802	arg1	plots					812:816	Van't Hoff plots	801:816	Van't Hoff plots	801:816	Van't Hoff plots were used to investigate thermodynamic parameters for enantiomers-stationary phase interaction and formation of inclusion complex.					
35322921	1	47	theme	liquid	348:353	arg1	chromatography					355:368	reverse phase high-performance liquid chromatography	317:368	reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin	317:404	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	3	48	theme	L	753:753	arg1	mmol-1					755:760	2.90 × 10-3 and 1.00 × 10-4 L mmol-1	725:760	mmol-1	755:760	Apparent formation constant between methanol, acetonitrile, and sulfobutylether-β-cyclodextrin were determined to be 2.90 × 10-3 and 1.00 × 10-4 L mmol-1 under 25°C using UV-spectrophotometry.					
35322921	4	49	theme	inclusion	930:938	arg1	complex					940:946	inclusion complex	930:946	inclusion complex	930:946	Van't Hoff plots were used to investigate thermodynamic parameters for enantiomers-stationary phase interaction and formation of inclusion complex.					
35322921	6	50	theme	second	1101:1106	arg1	model					1108:1112	The second model	1097:1112	The second model with complex adsorption	1097:1136	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	4	51	theme	phase	895:899	arg1	interaction					901:911	enantiomers-stationary phase interaction	872:911	enantiomers-stationary phase interaction	872:911	Van't Hoff plots were used to investigate thermodynamic parameters for enantiomers-stationary phase interaction and formation of inclusion complex.					
35322921	4	52	theme	Hoff	807:810	arg1	plots					812:816	Van't Hoff plots	801:816	Van't Hoff plots	801:816	Van't Hoff plots were used to investigate thermodynamic parameters for enantiomers-stationary phase interaction and formation of inclusion complex.					
35322921	3	53	theme	×	746:746	arg1	mmol-1					755:760	2.90 × 10-3 and 1.00 × 10-4 L mmol-1	725:760	mmol-1	755:760	Apparent formation constant between methanol, acetonitrile, and sulfobutylether-β-cyclodextrin were determined to be 2.90 × 10-3 and 1.00 × 10-4 L mmol-1 under 25°C using UV-spectrophotometry.					
35322921	4	54	theme	thermodynamic	843:855	arg1	parameters					857:866	thermodynamic parameters	843:866	thermodynamic parameters for enantiomers-stationary phase interaction and formation of inclusion complex	843:946	Van't Hoff plots were used to investigate thermodynamic parameters for enantiomers-stationary phase interaction and formation of inclusion complex.					
35322921	1	55	theme	Analytical	142:151	arg1	phase					423:427	chiral mobile phase additive	409:436	chiral mobile phase additive	409:436	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	1	55	theme	Analytical	142:151	arg1	enantioseparations					153:170	Analytical enantioseparations	142:170	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate,	142:294	Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans-paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive.					
35322921	7	56	theme	mobile	1391:1396	arg1	phase					1398:1402	the selected mobile phase	1378:1402	the selected mobile phase	1378:1402	In the selected mobile phase, stoichiometric ratio for both of inclusion complex was found to be 1:1.					
35322921	6	57	theme	fluoxetine	1185:1194	arg1	hydrochloride					1196:1208	fluoxetine hydrochloride	1185:1208	fluoxetine hydrochloride	1185:1208	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	6	58	theme	sulfate	1366:1372	arg1	behaviors					1326:1334	the retention behaviors	1312:1334	the retention behaviors of trans-paroxol and atropine sulfate	1312:1372	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	6	59	theme	venlafaxine	1226:1236	arg1	enantiomers					1252:1262	venlafaxine hydrochloride enantiomers	1226:1262	venlafaxine hydrochloride enantiomers	1226:1262	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	6	60	theme	retention	1163:1171	arg1	behavior					1173:1180	the retention behavior	1159:1180	the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers	1159:1262	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	0	61	theme	phase	59:63	arg1	HPLC					65:68	reverse phase HPLC	51:68	reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive	51:139	Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive.					
35322921	6	62	theme	atropine	1357:1364	arg1	sulfate					1366:1372	atropine sulfate	1357:1372	atropine sulfate	1357:1372	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35322921	4	63	used	used	823:826	arg2	plots					812:816	Van't Hoff plots	801:816	Van't Hoff plots	801:816	Van't Hoff plots were used to investigate thermodynamic parameters for enantiomers-stationary phase interaction and formation of inclusion complex.					
35322921	6	64	with	consistent	1296:1305	arg1	behaviors					1326:1334	the retention behaviors	1312:1334	the retention behaviors of trans-paroxol and atropine sulfate	1312:1372	The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans-paroxol and atropine sulfate.					
35736247	3	0	theme	potential	506:514	arg1	glycobiomarkers					516:530	potential glycobiomarkers	506:530	potential glycobiomarkers of disease	506:541	Here, we discuss about the importance of glycobiology in modern medicine, taking into account the impact of altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease, as well as on cancer development and progression.					
35736247	3	1	from	importance	370:379	arg1	medicine					407:414	modern medicine	400:414	modern medicine	400:414	Here, we discuss about the importance of glycobiology in modern medicine, taking into account the impact of altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease, as well as on cancer development and progression.					
35736247	1	2	theme	Cancer	47:52	arg1	development					54:64	Cancer development	47:64	Cancer development	47:64	Cancer development and progression is associated with aberrant changes in cellular glycosylation.					
35736247	3	3	theme	cancer	490:495	arg1	cells					497:501	cancer cells	490:501	cancer cells	490:501	Here, we discuss about the importance of glycobiology in modern medicine, taking into account the impact of altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease, as well as on cancer development and progression.					
35736247	2	4	theme	processes	278:286	arg1	induction					247:255	the induction	243:255	the induction of inhibitory immune processes which subsequently promote tumor growth and spreading	243:340	Cells expressing altered glycan-structures are recognized by cells of the immune system, favoring the induction of inhibitory immune processes which subsequently promote tumor growth and spreading.					
35736247	2	5	theme	immune	271:276	arg1	processes					278:286	inhibitory immune processes	260:286	inhibitory immune processes which subsequently promote tumor growth and spreading	260:340	Cells expressing altered glycan-structures are recognized by cells of the immune system, favoring the induction of inhibitory immune processes which subsequently promote tumor growth and spreading.					
35736247	3	6	theme	glycobiology	384:395	arg1	importance					370:379	the importance	366:379	the importance of glycobiology in modern medicine	366:414	Here, we discuss about the importance of glycobiology in modern medicine, taking into account the impact of altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease, as well as on cancer development and progression.					
35736247	3	7	theme	cancer	558:563	arg1	development					565:575	cancer development	558:575	cancer development	558:575	Here, we discuss about the importance of glycobiology in modern medicine, taking into account the impact of altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease, as well as on cancer development and progression.					
35736247	1	8	from	changes	110:116	arg1	glycosylation					130:142	cellular glycosylation	121:142	cellular glycosylation	121:142	Cancer development and progression is associated with aberrant changes in cellular glycosylation.					
35736247	2	9	theme	tumor	315:319	arg1	growth					321:326	tumor growth	315:326	tumor growth	315:326	Cells expressing altered glycan-structures are recognized by cells of the immune system, favoring the induction of inhibitory immune processes which subsequently promote tumor growth and spreading.					
35736247	3	10	theme	glycan	459:464	arg1	structures					466:475	altered glycan structures	451:475	altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease	451:541	Here, we discuss about the importance of glycobiology in modern medicine, taking into account the impact of altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease, as well as on cancer development and progression.					
35736247	0	11	theme	Cancer	16:21	arg1	Sugar					24:28	Cancer: Sugar	16:28	Cancer: Sugar	16:28	Glycobiology of Cancer: Sugar Drives the Show.					
35736247	3	12	theme	disease	535:541	arg1	glycobiomarkers					516:530	potential glycobiomarkers	506:530	potential glycobiomarkers of disease	506:541	Here, we discuss about the importance of glycobiology in modern medicine, taking into account the impact of altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease, as well as on cancer development and progression.					
35736247	3	13	theme	structures	466:475	arg1	impact					441:446	the impact	437:446	the impact of altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease	437:541	Here, we discuss about the importance of glycobiology in modern medicine, taking into account the impact of altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease, as well as on cancer development and progression.					
35736247	0	14	theme	Sugar	24:28	arg1	Glycobiology					0:11	Glycobiology	0:11	Glycobiology of Cancer: Sugar	0:28	Glycobiology of Cancer: Sugar Drives the Show.					
35736247	0	15	dep	the	37:39	arg1	Show					41:44	Show	41:44	Show	41:44	Glycobiology of Cancer: Sugar Drives the Show.					
35736247	3	16	theme	altered	451:457	arg1	structures					466:475	altered glycan structures	451:475	altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease	451:541	Here, we discuss about the importance of glycobiology in modern medicine, taking into account the impact of altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease, as well as on cancer development and progression.					
35736247	3	17	theme	modern	400:405	arg1	medicine					407:414	modern medicine	400:414	modern medicine	400:414	Here, we discuss about the importance of glycobiology in modern medicine, taking into account the impact of altered glycan structures expressed in cancer cells as potential glycobiomarkers of disease, as well as on cancer development and progression.					
35736247	2	18	theme	altered	162:168	arg1	glycan-structures					170:186	altered glycan-structures	162:186	altered glycan-structures	162:186	Cells expressing altered glycan-structures are recognized by cells of the immune system, favoring the induction of inhibitory immune processes which subsequently promote tumor growth and spreading.					
35736247	2	19	theme	system	226:231	arg1	cells					206:210	cells	206:210	cells of the immune system	206:231	Cells expressing altered glycan-structures are recognized by cells of the immune system, favoring the induction of inhibitory immune processes which subsequently promote tumor growth and spreading.					
35736247	1	20	theme	aberrant	101:108	arg1	changes					110:116	aberrant changes	101:116	aberrant changes in cellular glycosylation	101:142	Cancer development and progression is associated with aberrant changes in cellular glycosylation.					
35736247	2	21	theme	immune	219:224	arg1	system					226:231	the immune system	215:231	the immune system	215:231	Cells expressing altered glycan-structures are recognized by cells of the immune system, favoring the induction of inhibitory immune processes which subsequently promote tumor growth and spreading.					
35736247	2	22	theme	inhibitory	260:269	arg1	processes					278:286	inhibitory immune processes	260:286	inhibitory immune processes which subsequently promote tumor growth and spreading	260:340	Cells expressing altered glycan-structures are recognized by cells of the immune system, favoring the induction of inhibitory immune processes which subsequently promote tumor growth and spreading.					
35736247	1	23	theme	cellular	121:128	arg1	glycosylation					130:142	cellular glycosylation	121:142	cellular glycosylation	121:142	Cancer development and progression is associated with aberrant changes in cellular glycosylation.					
37365203	0	0	theme	structural	73:82	arg1	motifs					84:89	common structural motifs	66:89	common structural motifs	66:89	Creation of a milk oligosaccharide database, MilkOligoDB, reveals common structural motifs and extensive diversity across mammals.					
37365203	5	1	theme	unique	947:952	arg1	structures					970:979	783 unique oligosaccharide structures	943:979	783 unique oligosaccharide structures from the milk of 77 different species harvested from 113 publications	943:1049	The resulting database, MilkOligoDB, includes 3193 entries for 783 unique oligosaccharide structures from the milk of 77 different species harvested from 113 publications.					
37365203	1	2	theme	structures	246:255	arg1	range					237:241	a range	235:241	a range of structures and monosaccharide compositions	235:287	The carbohydrate fraction of most mammalian milks contains a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions.					
37365203	0	3	theme	common	66:71	arg1	motifs					84:89	common structural motifs	66:89	common structural motifs	66:89	Creation of a milk oligosaccharide database, MilkOligoDB, reveals common structural motifs and extensive diversity across mammals.					
37365203	1	4	theme	most	160:163	arg1	milks					175:179	most mammalian milks	160:179	most mammalian milks	160:179	The carbohydrate fraction of most mammalian milks contains a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions.					
37365203	5	5	from	milk	990:993	arg1	structures					970:979	783 unique oligosaccharide structures	943:979	783 unique oligosaccharide structures from the milk of 77 different species harvested from 113 publications	943:1049	The resulting database, MilkOligoDB, includes 3193 entries for 783 unique oligosaccharide structures from the milk of 77 different species harvested from 113 publications.					
37365203	8	6	theme	diverse	1451:1457	arg1	oligosaccharides					1459:1474	diverse oligosaccharides	1451:1474	diverse oligosaccharides that may be valuable for human supplementation	1451:1521	However, agriculturally important species do produce diverse oligosaccharides that may be valuable for human supplementation.					
37365203	5	7	theme	species	1011:1017	arg1	milk					990:993	the milk	986:993	the milk of 77 different species harvested from 113 publications	986:1049	The resulting database, MilkOligoDB, includes 3193 entries for 783 unique oligosaccharide structures from the milk of 77 different species harvested from 113 publications.					
37365203	1	8	theme	monosaccharide	261:274	arg1	compositions					276:287	monosaccharide compositions	261:287	monosaccharide compositions	261:287	The carbohydrate fraction of most mammalian milks contains a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions.					
37365203	7	9	theme	fucosylation	1295:1306	arg1	combination					1280:1290	the specific combination	1267:1290	the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides	1267:1395	Of the species studied, only chimpanzees, bonobos, and Asian elephants share the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides.					
37365203	4	10	theme	comprehensive	788:800	arg1	database					820:827	a comprehensive, machine-readable database	786:827	a comprehensive, machine-readable database of milk oligosaccharides across mammalian species	786:877	In the present study, publications on milk oligosaccharide profiles were identified and harmonized into a standardized format to create a comprehensive, machine-readable database of milk oligosaccharides across mammalian species.					
37365203	7	11	theme	structures	1331:1340	arg1	combination					1280:1290	the specific combination	1267:1290	the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides	1267:1395	Of the species studied, only chimpanzees, bonobos, and Asian elephants share the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides.					
37365203	2	12	theme	milk	296:299	arg1	oligosaccharides					301:316	Human milk oligosaccharides	290:316	Human milk oligosaccharides	290:316	Human milk oligosaccharides have received considerable attention due to their biological roles in neonatal gut microbiota, immunomodulation, and brain development.					
37365203	9	13	theme	milk	1608:1611	arg1	profiles					1629:1636	milk oligosaccharide profiles	1608:1636	milk oligosaccharide profiles	1608:1636	Overall, MilkOligoDB facilitates cross-species and cross-publication comparisons of milk oligosaccharide profiles and the generation of new data-driven hypotheses for future research.					
37365203	1	14	theme	compositions	276:287	arg1	range					237:241	a range	235:241	a range of structures and monosaccharide compositions	235:287	The carbohydrate fraction of most mammalian milks contains a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions.					
37365203	7	15	theme	specific	1271:1278	arg1	combination					1280:1290	the specific combination	1267:1290	the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides	1267:1395	Of the species studied, only chimpanzees, bonobos, and Asian elephants share the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides.					
37365203	3	16	theme	milk	513:516	arg1	oligosaccharides					518:533	milk oligosaccharides	513:533	milk oligosaccharides	513:533	However, a major challenge in understanding the biology of milk oligosaccharides across other mammals is that reports span more than 5 decades of publications with varying data reporting methods.					
37365203	6	17	theme	common	1140:1145	arg1	motifs					1158:1163	common structural motifs	1140:1163	common structural motifs within mammalian orders	1140:1187	Cross-species and cross-publication comparisons of milk oligosaccharide profiles reveal common structural motifs within mammalian orders.					
37365203	2	18	theme	Human	290:294	arg1	oligosaccharides					301:316	Human milk oligosaccharides	290:316	Human milk oligosaccharides	290:316	Human milk oligosaccharides have received considerable attention due to their biological roles in neonatal gut microbiota, immunomodulation, and brain development.					
37365203	1	19	theme	mammalian	165:173	arg1	milks					175:179	most mammalian milks	160:179	most mammalian milks	160:179	The carbohydrate fraction of most mammalian milks contains a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions.					
37365203	0	20	theme	extensive	95:103	arg1	diversity					105:113	extensive diversity	95:113	extensive diversity across mammals	95:128	Creation of a milk oligosaccharide database, MilkOligoDB, reveals common structural motifs and extensive diversity across mammals.					
37365203	5	21	theme	oligosaccharide	954:968	arg1	structures					970:979	783 unique oligosaccharide structures	943:979	783 unique oligosaccharide structures from the milk of 77 different species harvested from 113 publications	943:1049	The resulting database, MilkOligoDB, includes 3193 entries for 783 unique oligosaccharide structures from the milk of 77 different species harvested from 113 publications.					
37365203	6	22	theme	cross-publication	1070:1086	arg1	comparisons					1088:1098	Cross-species and cross-publication comparisons	1052:1098	comparisons	1088:1098	Cross-species and cross-publication comparisons of milk oligosaccharide profiles reveal common structural motifs within mammalian orders.					
37365203	9	23	theme	data-driven	1664:1674	arg1	hypotheses					1676:1685	new data-driven hypotheses	1660:1685	new data-driven hypotheses for future research	1660:1705	Overall, MilkOligoDB facilitates cross-species and cross-publication comparisons of milk oligosaccharide profiles and the generation of new data-driven hypotheses for future research.					
37365203	1	24	theme	oligosaccharides	203:218	arg1	oligosaccharides					203:218	oligosaccharides	203:218	oligosaccharides that encompass a range of structures and monosaccharide compositions	203:287	The carbohydrate fraction of most mammalian milks contains a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions.					
37365203	1	24	theme	oligosaccharides	203:218	arg1	variety					192:198	a variety	190:198	a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions	190:287	The carbohydrate fraction of most mammalian milks contains a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions.					
37365203	1	25	theme	milks	175:179	arg1	fraction					148:155	The carbohydrate fraction	131:155	The carbohydrate fraction of most mammalian milks	131:179	The carbohydrate fraction of most mammalian milks contains a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions.					
37365203	7	26	theme	sialylation	1309:1319	arg1	combination					1280:1290	the specific combination	1267:1290	the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides	1267:1395	Of the species studied, only chimpanzees, bonobos, and Asian elephants share the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides.					
37365203	7	27	theme	oligosaccharides	1380:1395	arg1	characteristic					1351:1364	characteristic	1351:1364	characteristic	1351:1364	Of the species studied, only chimpanzees, bonobos, and Asian elephants share the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides.					
37365203	2	28	from	roles	379:383	arg1	immunomodulation					413:428	immunomodulation	413:428	immunomodulation	413:428	Human milk oligosaccharides have received considerable attention due to their biological roles in neonatal gut microbiota, immunomodulation, and brain development.					
37365203	2	28	from	roles	379:383	arg1	microbiota					401:410	neonatal gut microbiota	388:410	neonatal gut microbiota	388:410	Human milk oligosaccharides have received considerable attention due to their biological roles in neonatal gut microbiota, immunomodulation, and brain development.					
37365203	2	28	from	roles	379:383	arg1	development					441:451	brain development	435:451	brain development	435:451	Human milk oligosaccharides have received considerable attention due to their biological roles in neonatal gut microbiota, immunomodulation, and brain development.					
37365203	4	29	from	publications	672:683	arg1	profiles					709:716	milk oligosaccharide profiles	688:716	milk oligosaccharide profiles	688:716	In the present study, publications on milk oligosaccharide profiles were identified and harmonized into a standardized format to create a comprehensive, machine-readable database of milk oligosaccharides across mammalian species.					
37365203	4	30	theme	oligosaccharide	693:707	arg1	profiles					709:716	milk oligosaccharide profiles	688:716	milk oligosaccharide profiles	688:716	In the present study, publications on milk oligosaccharide profiles were identified and harmonized into a standardized format to create a comprehensive, machine-readable database of milk oligosaccharides across mammalian species.					
37365203	2	31	theme	considerable	332:343	arg1	attention					345:353	considerable attention	332:353	considerable attention	332:353	Human milk oligosaccharides have received considerable attention due to their biological roles in neonatal gut microbiota, immunomodulation, and brain development.					
37365203	4	32	theme	milk	688:691	arg1	profiles					709:716	milk oligosaccharide profiles	688:716	milk oligosaccharide profiles	688:716	In the present study, publications on milk oligosaccharide profiles were identified and harmonized into a standardized format to create a comprehensive, machine-readable database of milk oligosaccharides across mammalian species.					
37365203	0	33	theme	milk	14:17	arg1	database					35:42	a milk oligosaccharide database	12:42	a milk oligosaccharide database	12:42	Creation of a milk oligosaccharide database, MilkOligoDB, reveals common structural motifs and extensive diversity across mammals.					
37365203	0	33	theme	milk	14:17	arg1	MilkOligoDB					45:55	MilkOligoDB	45:55	MilkOligoDB	45:55	Creation of a milk oligosaccharide database, MilkOligoDB, reveals common structural motifs and extensive diversity across mammals.					
37365203	6	34	theme	mammalian	1172:1180	arg1	orders					1182:1187	mammalian orders	1172:1187	mammalian orders	1172:1187	Cross-species and cross-publication comparisons of milk oligosaccharide profiles reveal common structural motifs within mammalian orders.					
37365203	5	35	theme	different	1001:1009	arg1	species					1011:1017	77 different species	998:1017	77 different species harvested from 113 publications	998:1049	The resulting database, MilkOligoDB, includes 3193 entries for 783 unique oligosaccharide structures from the milk of 77 different species harvested from 113 publications.					
37365203	4	36	theme	present	657:663	arg1	study					665:669	the present study	653:669	the present study	653:669	In the present study, publications on milk oligosaccharide profiles were identified and harmonized into a standardized format to create a comprehensive, machine-readable database of milk oligosaccharides across mammalian species.					
37365203	5	37	theme	77	998:999	arg1	species					1011:1017	77 different species	998:1017	77 different species harvested from 113 publications	998:1049	The resulting database, MilkOligoDB, includes 3193 entries for 783 unique oligosaccharide structures from the milk of 77 different species harvested from 113 publications.					
37365203	9	38	theme	profiles	1629:1636	arg1	comparisons					1593:1603	cross-species and cross-publication comparisons	1557:1603	comparisons	1593:1603	Overall, MilkOligoDB facilitates cross-species and cross-publication comparisons of milk oligosaccharide profiles and the generation of new data-driven hypotheses for future research.					
37365203	9	38	theme	profiles	1629:1636	arg1	generation					1646:1655	the generation	1642:1655	the generation of new data-driven hypotheses for future research	1642:1705	Overall, MilkOligoDB facilitates cross-species and cross-publication comparisons of milk oligosaccharide profiles and the generation of new data-driven hypotheses for future research.					
37365203	8	39	theme	human	1501:1505	arg1	supplementation					1507:1521	human supplementation	1501:1521	human supplementation	1501:1521	However, agriculturally important species do produce diverse oligosaccharides that may be valuable for human supplementation.					
37365203	3	40	theme	other	542:546	arg1	mammals					548:554	other mammals	542:554	other mammals	542:554	However, a major challenge in understanding the biology of milk oligosaccharides across other mammals is that reports span more than 5 decades of publications with varying data reporting methods.					
37365203	9	41	theme	cross-species	1557:1569	arg1	comparisons					1593:1603	cross-species and cross-publication comparisons	1557:1603	comparisons	1593:1603	Overall, MilkOligoDB facilitates cross-species and cross-publication comparisons of milk oligosaccharide profiles and the generation of new data-driven hypotheses for future research.					
37365203	1	42	theme	carbohydrate	135:146	arg1	fraction					148:155	The carbohydrate fraction	131:155	The carbohydrate fraction of most mammalian milks	131:179	The carbohydrate fraction of most mammalian milks contains a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions.					
37365203	0	43	theme	database	35:42	arg1	Creation					0:7	Creation	0:7	Creation of a milk oligosaccharide database, MilkOligoDB,	0:56	Creation of a milk oligosaccharide database, MilkOligoDB, reveals common structural motifs and extensive diversity across mammals.					
37365203	7	44	theme	milk	1375:1378	arg1	oligosaccharides					1380:1395	human milk oligosaccharides	1369:1395	human milk oligosaccharides	1369:1395	Of the species studied, only chimpanzees, bonobos, and Asian elephants share the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides.					
37365203	1	45	contain	contains	181:188	arg1	fraction					148:155	The carbohydrate fraction	131:155	The carbohydrate fraction of most mammalian milks	131:179	The carbohydrate fraction of most mammalian milks contains a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions.					
37365203	1	45	contain	contains	181:188	arg2	oligosaccharides					203:218	oligosaccharides	203:218	oligosaccharides that encompass a range of structures and monosaccharide compositions	203:287	The carbohydrate fraction of most mammalian milks contains a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions.					
37365203	1	45	contain	contains	181:188	arg2	variety					192:198	a variety	190:198	a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions	190:287	The carbohydrate fraction of most mammalian milks contains a variety of oligosaccharides that encompass a range of structures and monosaccharide compositions.					
37365203	8	46	theme	important	1422:1430	arg1	species					1432:1438	agriculturally important species	1407:1438	agriculturally important species	1407:1438	However, agriculturally important species do produce diverse oligosaccharides that may be valuable for human supplementation.					
37365203	2	47	theme	brain	435:439	arg1	development					441:451	brain development	435:451	brain development	435:451	Human milk oligosaccharides have received considerable attention due to their biological roles in neonatal gut microbiota, immunomodulation, and brain development.					
37365203	0	48	theme	oligosaccharide	19:33	arg1	database					35:42	a milk oligosaccharide database	12:42	a milk oligosaccharide database	12:42	Creation of a milk oligosaccharide database, MilkOligoDB, reveals common structural motifs and extensive diversity across mammals.					
37365203	0	48	theme	oligosaccharide	19:33	arg1	MilkOligoDB					45:55	MilkOligoDB	45:55	MilkOligoDB	45:55	Creation of a milk oligosaccharide database, MilkOligoDB, reveals common structural motifs and extensive diversity across mammals.					
37365203	9	49	theme	oligosaccharide	1613:1627	arg1	profiles					1629:1636	milk oligosaccharide profiles	1608:1636	milk oligosaccharide profiles	1608:1636	Overall, MilkOligoDB facilitates cross-species and cross-publication comparisons of milk oligosaccharide profiles and the generation of new data-driven hypotheses for future research.					
37365203	3	50	theme	data	626:629	arg1	methods					641:647	data reporting methods	626:647	data reporting methods	626:647	However, a major challenge in understanding the biology of milk oligosaccharides across other mammals is that reports span more than 5 decades of publications with varying data reporting methods.					
37365203	6	51	theme	profiles	1124:1131	arg1	comparisons					1088:1098	Cross-species and cross-publication comparisons	1052:1098	comparisons	1088:1098	Cross-species and cross-publication comparisons of milk oligosaccharide profiles reveal common structural motifs within mammalian orders.					
37365203	9	52	theme	cross-publication	1575:1591	arg1	comparisons					1593:1603	cross-species and cross-publication comparisons	1557:1603	comparisons	1593:1603	Overall, MilkOligoDB facilitates cross-species and cross-publication comparisons of milk oligosaccharide profiles and the generation of new data-driven hypotheses for future research.					
37365203	3	53	theme	publications	600:611	arg1	decades					589:595	more than 5 decades	577:595	more than 5 decades of publications	577:611	However, a major challenge in understanding the biology of milk oligosaccharides across other mammals is that reports span more than 5 decades of publications with varying data reporting methods.					
37365203	2	54	theme	biological	368:377	arg1	roles					379:383	their biological roles	362:383	their biological roles in neonatal gut microbiota, immunomodulation, and brain development	362:451	Human milk oligosaccharides have received considerable attention due to their biological roles in neonatal gut microbiota, immunomodulation, and brain development.					
37365203	9	55	theme	new	1660:1662	arg1	hypotheses					1676:1685	new data-driven hypotheses	1660:1685	new data-driven hypotheses for future research	1660:1705	Overall, MilkOligoDB facilitates cross-species and cross-publication comparisons of milk oligosaccharide profiles and the generation of new data-driven hypotheses for future research.					
37365203	9	56	theme	future	1691:1696	arg1	research					1698:1705	future research	1691:1705	future research	1691:1705	Overall, MilkOligoDB facilitates cross-species and cross-publication comparisons of milk oligosaccharide profiles and the generation of new data-driven hypotheses for future research.					
37365203	7	57	theme	human	1369:1373	arg1	oligosaccharides					1380:1395	human milk oligosaccharides	1369:1395	human milk oligosaccharides	1369:1395	Of the species studied, only chimpanzees, bonobos, and Asian elephants share the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides.					
37365203	3	58	theme	oligosaccharides	518:533	arg1	biology					502:508	the biology	498:508	the biology of milk oligosaccharides across other mammals	498:554	However, a major challenge in understanding the biology of milk oligosaccharides across other mammals is that reports span more than 5 decades of publications with varying data reporting methods.					
37365203	7	59	theme	Asian	1245:1249	arg1	elephants					1251:1259	Asian elephants	1245:1259	Asian elephants	1245:1259	Of the species studied, only chimpanzees, bonobos, and Asian elephants share the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides.					
37365203	4	60	dep	comprehensive	788:800	arg1	machine-readable					803:818	machine-readable	803:818	machine-readable	803:818	In the present study, publications on milk oligosaccharide profiles were identified and harmonized into a standardized format to create a comprehensive, machine-readable database of milk oligosaccharides across mammalian species.					
37365203	4	61	theme	milk	832:835	arg1	oligosaccharides					837:852	milk oligosaccharides	832:852	milk oligosaccharides	832:852	In the present study, publications on milk oligosaccharide profiles were identified and harmonized into a standardized format to create a comprehensive, machine-readable database of milk oligosaccharides across mammalian species.					
37365203	4	62	theme	mammalian	861:869	arg1	species					871:877	mammalian species	861:877	mammalian species	861:877	In the present study, publications on milk oligosaccharide profiles were identified and harmonized into a standardized format to create a comprehensive, machine-readable database of milk oligosaccharides across mammalian species.					
37365203	4	63	theme	standardized	756:767	arg1	format					769:774	a standardized format	754:774	a standardized format to create a comprehensive, machine-readable database of milk oligosaccharides across mammalian species	754:877	In the present study, publications on milk oligosaccharide profiles were identified and harmonized into a standardized format to create a comprehensive, machine-readable database of milk oligosaccharides across mammalian species.					
37365203	6	64	theme	oligosaccharide	1108:1122	arg1	profiles					1124:1131	milk oligosaccharide profiles	1103:1131	milk oligosaccharide profiles	1103:1131	Cross-species and cross-publication comparisons of milk oligosaccharide profiles reveal common structural motifs within mammalian orders.					
37365203	6	65	theme	structural	1147:1156	arg1	motifs					1158:1163	common structural motifs	1140:1163	common structural motifs within mammalian orders	1140:1187	Cross-species and cross-publication comparisons of milk oligosaccharide profiles reveal common structural motifs within mammalian orders.					
37365203	9	66	theme	hypotheses	1676:1685	arg1	comparisons					1593:1603	cross-species and cross-publication comparisons	1557:1603	comparisons	1593:1603	Overall, MilkOligoDB facilitates cross-species and cross-publication comparisons of milk oligosaccharide profiles and the generation of new data-driven hypotheses for future research.					
37365203	9	66	theme	hypotheses	1676:1685	arg1	generation					1646:1655	the generation	1642:1655	the generation of new data-driven hypotheses for future research	1642:1705	Overall, MilkOligoDB facilitates cross-species and cross-publication comparisons of milk oligosaccharide profiles and the generation of new data-driven hypotheses for future research.					
37365203	6	67	theme	milk	1103:1106	arg1	profiles					1124:1131	milk oligosaccharide profiles	1103:1131	milk oligosaccharide profiles	1103:1131	Cross-species and cross-publication comparisons of milk oligosaccharide profiles reveal common structural motifs within mammalian orders.					
37365203	3	68	theme	reporting	631:639	arg1	methods					641:647	data reporting methods	626:647	data reporting methods	626:647	However, a major challenge in understanding the biology of milk oligosaccharides across other mammals is that reports span more than 5 decades of publications with varying data reporting methods.					
37365203	5	69	theme	resulting	884:892	arg1	MilkOligoDB					904:914	MilkOligoDB	904:914	MilkOligoDB	904:914	The resulting database, MilkOligoDB, includes 3193 entries for 783 unique oligosaccharide structures from the milk of 77 different species harvested from 113 publications.					
37365203	5	69	theme	resulting	884:892	arg1	database					894:901	The resulting database	880:901	The resulting database	880:901	The resulting database, MilkOligoDB, includes 3193 entries for 783 unique oligosaccharide structures from the milk of 77 different species harvested from 113 publications.					
37365203	2	70	theme	gut	397:399	arg1	microbiota					401:410	neonatal gut microbiota	388:410	neonatal gut microbiota	388:410	Human milk oligosaccharides have received considerable attention due to their biological roles in neonatal gut microbiota, immunomodulation, and brain development.					
37365203	3	71	theme	major	465:469	arg1	challenge					471:479	a major challenge	463:479	a major challenge in understanding the biology of milk oligosaccharides across other mammals	463:554	However, a major challenge in understanding the biology of milk oligosaccharides across other mammals is that reports span more than 5 decades of publications with varying data reporting methods.					
37365203	6	72	theme	Cross-species	1052:1064	arg1	comparisons					1088:1098	Cross-species and cross-publication comparisons	1052:1098	comparisons	1088:1098	Cross-species and cross-publication comparisons of milk oligosaccharide profiles reveal common structural motifs within mammalian orders.					
37365203	4	73	theme	oligosaccharides	837:852	arg1	database					820:827	a comprehensive, machine-readable database	786:827	a comprehensive, machine-readable database of milk oligosaccharides across mammalian species	786:877	In the present study, publications on milk oligosaccharide profiles were identified and harmonized into a standardized format to create a comprehensive, machine-readable database of milk oligosaccharides across mammalian species.					
37365203	2	74	theme	neonatal	388:395	arg1	microbiota					401:410	neonatal gut microbiota	388:410	neonatal gut microbiota	388:410	Human milk oligosaccharides have received considerable attention due to their biological roles in neonatal gut microbiota, immunomodulation, and brain development.					
37365203	7	75	theme	core	1326:1329	arg1	structures					1331:1340	core structures	1326:1340	core structures that are characteristic of human milk oligosaccharides	1326:1395	Of the species studied, only chimpanzees, bonobos, and Asian elephants share the specific combination of fucosylation, sialylation, and core structures that are characteristic of human milk oligosaccharides.					
35839954	6	0	theme	hemostatic	963:972	arg1	experiment					974:983	the hemostatic experiment	959:983	the hemostatic experiment	959:983	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	3	1	theme	rapid	566:570	arg1	hemorrhage					588:597	rapid noncompressible hemorrhage	566:597	rapid noncompressible hemorrhage	566:597	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	2	theme	wound	617:621	arg1	infection					623:631	wound infection	617:631	wound infection	617:631	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	0	3	theme	Injectable	0:9	arg1	isolate					59:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate	0:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate	0:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	2	4	theme	hemostatic	354:363	arg1	materials					365:373	traditional hemostatic materials	342:373	traditional hemostatic materials	342:373	However, traditional hemostatic materials are ineffective for extreme bleeding and subsequent wound infection.					
35839954	1	5	theme	wound	231:235	arg1	hemorrhage					205:214	Uncontrollable hemorrhage	190:214	Uncontrollable hemorrhage	190:214	Uncontrollable hemorrhage and subsequent wound infection are severe threats to life, especially for the deep noncompressible massive bleeding.					
35839954	1	5	theme	wound	231:235	arg1	infection					237:245	subsequent wound infection	220:245	subsequent wound infection	220:245	Uncontrollable hemorrhage and subsequent wound infection are severe threats to life, especially for the deep noncompressible massive bleeding.					
35839954	1	5	theme	wound	231:235	arg1	threats					258:264	severe threats	251:264	severe threats to life	251:272	Uncontrollable hemorrhage and subsequent wound infection are severe threats to life, especially for the deep noncompressible massive bleeding.					
35839954	6	6	theme	Ag	1015:1016	arg1	sponge					1018:1023	EHP@Ag sponge	1011:1023	EHP@Ag sponge	1011:1023	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	4	7	theme	EHP	686:688	arg1	Ag					690:691	EHP@Ag	686:691	EHP@Ag	686:691	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	4	7	theme	EHP	686:688	arg1	sponge					678:683	The nano silver (AgNPs)-loaded shape memory sponge	634:683	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag)	634:692	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	9	8	theme	memory	1498:1503	arg1	sponges					1505:1511	the shape memory sponges	1488:1511	the shape memory sponges	1488:1511	In summary, the shape memory sponges can quickly control bleeding and avoid bacterial infection, which shows great potential for clinical application as a multifunctional hemostatic agent.					
35839954	6	9	theme	EHP	1011:1013	arg1	sponge					1018:1023	EHP@Ag sponge	1011:1023	EHP@Ag sponge	1011:1023	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	5	10	theme	shape	856:860	arg1	recovery					862:869	rapid blood-triggered shape recovery	834:869	rapid blood-triggered shape recovery	834:869	The EHP@Ag sponges showed rapid blood-triggered shape recovery speed, which is beneficial for administering noncompressible hemorrhage.					
35839954	0	11	theme	wound	173:177	arg1	infection					179:187	wound infection	173:187	wound infection	173:187	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	4	12	theme	reducing	767:774	arg1	AgNPs					793:797	then reducing and immobilizing AgNPs	762:797	then reducing and immobilizing AgNPs in situ	762:805	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	4	12	theme	reducing	767:774	arg1	sponge					754:759	EHSS sponge	749:759	EHSS sponge	749:759	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	6	13	theme	hemostasis	1066:1075	arg1	effect					1058:1063	a desirable hemostasis effect	1035:1063	a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg)	1035:1128	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	6	13	theme	hemostasis	1066:1075	arg1	time					1077:1080	hemostasis time	1066:1080	hemostasis time	1066:1080	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	7	14	theme	Ag	1257:1258	arg1	Meanwhile					1238:1246	Meanwhile	1238:1246	Meanwhile	1238:1246	Meanwhile, the EHP@Ag sponge has an efficient antibacterial property.					
35839954	7	14	theme	Ag	1257:1258	arg1	sponge					1260:1265	the EHP@Ag sponge	1249:1265	the EHP@Ag sponge	1249:1265	Meanwhile, the EHP@Ag sponge has an efficient antibacterial property.					
35839954	7	15	contain	has	1267:1269	arg1	Meanwhile					1238:1246	Meanwhile	1238:1246	Meanwhile	1238:1246	Meanwhile, the EHP@Ag sponge has an efficient antibacterial property.					
35839954	7	15	contain	has	1267:1269	arg2	property					1298:1305	an efficient antibacterial property	1271:1305	an efficient antibacterial property	1271:1305	Meanwhile, the EHP@Ag sponge has an efficient antibacterial property.					
35839954	7	15	contain	has	1267:1269	arg1	sponge					1260:1265	the EHP@Ag sponge	1249:1265	the EHP@Ag sponge	1249:1265	Meanwhile, the EHP@Ag sponge has an efficient antibacterial property.					
35839954	9	16	theme	bacterial	1552:1560	arg1	infection					1562:1570	bacterial infection	1552:1570	bacterial infection	1552:1570	In summary, the shape memory sponges can quickly control bleeding and avoid bacterial infection, which shows great potential for clinical application as a multifunctional hemostatic agent.					
35839954	4	17	theme	EHSS	749:752	arg1	AgNPs					793:797	then reducing and immobilizing AgNPs	762:797	then reducing and immobilizing AgNPs in situ	762:805	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	4	17	theme	EHSS	749:752	arg1	sponge					754:759	EHSS sponge	749:759	EHSS sponge	749:759	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	5	18	theme	EHP	812:814	arg1	sponges					819:825	The EHP@Ag sponges	808:825	The EHP@Ag sponges	808:825	The EHP@Ag sponges showed rapid blood-triggered shape recovery speed, which is beneficial for administering noncompressible hemorrhage.					
35839954	1	19	theme	Uncontrollable	190:203	arg1	hemorrhage					205:214	Uncontrollable hemorrhage	190:214	Uncontrollable hemorrhage	190:214	Uncontrollable hemorrhage and subsequent wound infection are severe threats to life, especially for the deep noncompressible massive bleeding.					
35839954	1	19	theme	Uncontrollable	190:203	arg1	infection					237:245	subsequent wound infection	220:245	subsequent wound infection	220:245	Uncontrollable hemorrhage and subsequent wound infection are severe threats to life, especially for the deep noncompressible massive bleeding.					
35839954	1	19	theme	Uncontrollable	190:203	arg1	threats					258:264	severe threats	251:264	severe threats to life	251:272	Uncontrollable hemorrhage and subsequent wound infection are severe threats to life, especially for the deep noncompressible massive bleeding.					
35839954	6	20	theme	desirable	1037:1045	arg1	effect					1058:1063	a desirable hemostasis effect	1035:1063	a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg)	1035:1128	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	6	20	theme	desirable	1037:1045	arg1	time					1077:1080	hemostasis time	1066:1080	hemostasis time	1066:1080	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	8	21	theme	EHP	1374:1376	arg1	sponges					1381:1387	the EHP@Ag sponges	1370:1387	the EHP@Ag sponges	1370:1387	Furthermore, the antibacterial experiment in vivo showed that the EHP@Ag sponges could kill bacteria effectively and reduce the bacteria-induced inflammatory response.					
35839954	8	22	theme	Ag	1378:1379	arg1	sponges					1381:1387	the EHP@Ag sponges	1370:1387	the EHP@Ag sponges	1370:1387	Furthermore, the antibacterial experiment in vivo showed that the EHP@Ag sponges could kill bacteria effectively and reduce the bacteria-induced inflammatory response.					
35839954	3	23	theme	hydroxyethyl	489:500	arg1	EHSS					556:559	EHSS	556:559	EHSS	556:559	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	23	theme	hydroxyethyl	489:500	arg1	sponge					548:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge	462:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection	462:631	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	0	24	theme	rapid	122:126	arg1	hemorrhage					144:153	rapid noncompressible hemorrhage	122:153	rapid noncompressible hemorrhage	122:153	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	0	25	theme	protein	51:57	arg1	isolate					59:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate	0:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate	0:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	4	26	theme	immobilizing	780:791	arg1	AgNPs					793:797	then reducing and immobilizing AgNPs	762:797	then reducing and immobilizing AgNPs in situ	762:805	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	4	26	theme	immobilizing	780:791	arg1	sponge					754:759	EHSS sponge	749:759	EHSS sponge	749:759	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	3	27	theme	shape	476:480	arg1	EHSS					556:559	EHSS	556:559	EHSS	556:559	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	27	theme	shape	476:480	arg1	sponge					548:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge	462:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection	462:631	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	5	28	theme	blood-triggered	840:854	arg1	recovery					862:869	rapid blood-triggered shape recovery	834:869	rapid blood-triggered shape recovery	834:869	The EHP@Ag sponges showed rapid blood-triggered shape recovery speed, which is beneficial for administering noncompressible hemorrhage.					
35839954	9	29	theme	clinical	1605:1612	arg1	application					1614:1624	clinical application	1605:1624	clinical application as a multifunctional hemostatic agent	1605:1662	In summary, the shape memory sponges can quickly control bleeding and avoid bacterial infection, which shows great potential for clinical application as a multifunctional hemostatic agent.					
35839954	4	30	theme	in	799:800	arg1	AgNPs					793:797	then reducing and immobilizing AgNPs	762:797	then reducing and immobilizing AgNPs in situ	762:805	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	4	30	theme	in	799:800	arg1	sponge					754:759	EHSS sponge	749:759	EHSS sponge	749:759	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	3	31	theme	based	532:536	arg1	EHSS					556:559	EHSS	556:559	EHSS	556:559	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	31	theme	based	532:536	arg1	sponge					548:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge	462:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection	462:631	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	0	32	theme	memory	17:22	arg1	isolate					59:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate	0:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate	0:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	33	theme	protein	516:522	arg1	EHSS					556:559	EHSS	556:559	EHSS	556:559	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	33	theme	protein	516:522	arg1	sponge					548:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge	462:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection	462:631	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	0	34	theme	cellulose/soy	37:49	arg1	isolate					59:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate	0:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate	0:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	5	35	theme	Ag	816:817	arg1	sponges					819:825	The EHP@Ag sponges	808:825	The EHP@Ag sponges	808:825	The EHP@Ag sponges showed rapid blood-triggered shape recovery speed, which is beneficial for administering noncompressible hemorrhage.					
35839954	9	36	theme	multifunctional	1631:1645	arg1	agent					1658:1662	a multifunctional hemostatic agent	1629:1662	a multifunctional hemostatic agent	1629:1662	In summary, the shape memory sponges can quickly control bleeding and avoid bacterial infection, which shows great potential for clinical application as a multifunctional hemostatic agent.					
35839954	0	37	theme	composite	73:81	arg1	sponge					83:88	composite sponge	73:88	composite sponge	73:88	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	8	38	theme	inflammatory	1453:1464	arg1	response					1466:1473	the bacteria-induced inflammatory response	1432:1473	the bacteria-induced inflammatory response	1432:1473	Furthermore, the antibacterial experiment in vivo showed that the EHP@Ag sponges could kill bacteria effectively and reduce the bacteria-induced inflammatory response.					
35839954	6	39	theme	gelatin	1157:1163	arg1	sponge					1165:1170	the commercial gelatin sponge	1142:1170	the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg)	1142:1235	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	6	39	theme	gelatin	1157:1163	arg1	time					1184:1187	hemostasis time	1173:1187	hemostasis time	1173:1187	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	4	40	theme	-loaded	657:663	arg1	Ag					690:691	EHP@Ag	686:691	EHP@Ag	686:691	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	4	40	theme	-loaded	657:663	arg1	sponge					678:683	The nano silver (AgNPs)-loaded shape memory sponge	634:683	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag)	634:692	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	3	41	theme	noncompressible	572:586	arg1	hemorrhage					588:597	rapid noncompressible hemorrhage	566:597	rapid noncompressible hemorrhage	566:597	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	6	42	theme	experiment	974:983	arg1	results					948:954	The results	944:954	The results of the hemostatic experiment in vivo	944:991	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	7	43	theme	efficient	1274:1282	arg1	property					1298:1305	an efficient antibacterial property	1271:1305	an efficient antibacterial property	1271:1305	Meanwhile, the EHP@Ag sponge has an efficient antibacterial property.					
35839954	4	44	theme	memory	671:676	arg1	Ag					690:691	EHP@Ag	686:691	EHP@Ag	686:691	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	4	44	theme	memory	671:676	arg1	sponge					678:683	The nano silver (AgNPs)-loaded shape memory sponge	634:683	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag)	634:692	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	6	45	from	results	948:954	arg1	vivo					988:991	vivo	988:991	vivo	988:991	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	6	46	theme	blood	1206:1210	arg1	loss					1212:1215	blood loss	1206:1215	blood loss	1206:1215	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	6	47	dep	time	1184:1187	arg1	49.25 ± 3.30 s					1190:1203	49.25 ± 3.30 s	1190:1203	49.25 ± 3.30 s	1190:1203	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	6	47	dep	time	1184:1187	arg1	loss					1212:1215	blood loss	1206:1215	blood loss	1206:1215	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	6	47	dep	time	1184:1187	arg1	755.50 ± 24.45 mg					1218:1234	755.50 ± 24.45 mg	1218:1234	755.50 ± 24.45 mg	1218:1234	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	7	48	theme	EHP	1253:1255	arg1	Meanwhile					1238:1246	Meanwhile	1238:1246	Meanwhile	1238:1246	Meanwhile, the EHP@Ag sponge has an efficient antibacterial property.					
35839954	7	48	theme	EHP	1253:1255	arg1	sponge					1260:1265	the EHP@Ag sponge	1249:1265	the EHP@Ag sponge	1249:1265	Meanwhile, the EHP@Ag sponge has an efficient antibacterial property.					
35839954	1	49	theme	subsequent	220:229	arg1	hemorrhage					205:214	Uncontrollable hemorrhage	190:214	Uncontrollable hemorrhage	190:214	Uncontrollable hemorrhage and subsequent wound infection are severe threats to life, especially for the deep noncompressible massive bleeding.					
35839954	1	49	theme	subsequent	220:229	arg1	infection					237:245	subsequent wound infection	220:245	subsequent wound infection	220:245	Uncontrollable hemorrhage and subsequent wound infection are severe threats to life, especially for the deep noncompressible massive bleeding.					
35839954	1	49	theme	subsequent	220:229	arg1	threats					258:264	severe threats	251:264	severe threats to life	251:272	Uncontrollable hemorrhage and subsequent wound infection are severe threats to life, especially for the deep noncompressible massive bleeding.					
35839954	2	50	theme	traditional	342:352	arg1	materials					365:373	traditional hemostatic materials	342:373	traditional hemostatic materials	342:373	However, traditional hemostatic materials are ineffective for extreme bleeding and subsequent wound infection.					
35839954	9	51	theme	shape	1492:1496	arg1	sponges					1505:1511	the shape memory sponges	1488:1511	the shape memory sponges	1488:1511	In summary, the shape memory sponges can quickly control bleeding and avoid bacterial infection, which shows great potential for clinical application as a multifunctional hemostatic agent.					
35839954	6	52	theme	@	1014:1014	arg1	sponge					1018:1023	EHP@Ag sponge	1011:1023	EHP@Ag sponge	1011:1023	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	2	53	theme	wound	427:431	arg1	infection					433:441	subsequent wound infection	416:441	subsequent wound infection	416:441	However, traditional hemostatic materials are ineffective for extreme bleeding and subsequent wound infection.					
35839954	5	54	theme	noncompressible	916:930	arg1	hemorrhage					932:941	noncompressible hemorrhage	916:941	noncompressible hemorrhage	916:941	The EHP@Ag sponges showed rapid blood-triggered shape recovery speed, which is beneficial for administering noncompressible hemorrhage.					
35839954	1	55	theme	severe	251:256	arg1	hemorrhage					205:214	Uncontrollable hemorrhage	190:214	Uncontrollable hemorrhage	190:214	Uncontrollable hemorrhage and subsequent wound infection are severe threats to life, especially for the deep noncompressible massive bleeding.					
35839954	1	55	theme	severe	251:256	arg1	infection					237:245	subsequent wound infection	220:245	subsequent wound infection	220:245	Uncontrollable hemorrhage and subsequent wound infection are severe threats to life, especially for the deep noncompressible massive bleeding.					
35839954	1	55	theme	severe	251:256	arg1	threats					258:264	severe threats	251:264	severe threats to life	251:272	Uncontrollable hemorrhage and subsequent wound infection are severe threats to life, especially for the deep noncompressible massive bleeding.					
35839954	4	56	theme	@	689:689	arg1	Ag					690:691	EHP@Ag	686:691	EHP@Ag	686:691	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	4	56	theme	@	689:689	arg1	sponge					678:683	The nano silver (AgNPs)-loaded shape memory sponge	634:683	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag)	634:692	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	0	57	theme	infection	179:187	arg1	prevention					159:168	prevention	159:168	prevention of wound infection	159:187	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	0	57	theme	infection	179:187	arg1	hemorrhage					144:153	rapid noncompressible hemorrhage	122:153	rapid noncompressible hemorrhage	122:153	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	4	58	dep	in	799:800	arg1	situ					802:805	situ	802:805	situ	802:805	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	3	59	theme	infection	623:631	arg1	prevention					603:612	prevention	603:612	prevention of wound infection	603:631	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	59	theme	infection	623:631	arg1	hemorrhage					588:597	rapid noncompressible hemorrhage	566:597	rapid noncompressible hemorrhage	566:597	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	8	60	theme	antibacterial	1325:1337	arg1	experiment					1339:1348	the antibacterial experiment	1321:1348	the antibacterial experiment in vivo	1321:1356	Furthermore, the antibacterial experiment in vivo showed that the EHP@Ag sponges could kill bacteria effectively and reduce the bacteria-induced inflammatory response.					
35839954	7	61	theme	@	1256:1256	arg1	Meanwhile					1238:1246	Meanwhile	1238:1246	Meanwhile	1238:1246	Meanwhile, the EHP@Ag sponge has an efficient antibacterial property.					
35839954	7	61	theme	@	1256:1256	arg1	sponge					1260:1265	the EHP@Ag sponge	1249:1265	the EHP@Ag sponge	1249:1265	Meanwhile, the EHP@Ag sponge has an efficient antibacterial property.					
35839954	4	62	theme	mussel-inspired	712:726	arg1	polydopamine					728:739	mussel-inspired polydopamine	712:739	mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ	712:805	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	0	63	theme	antibacterial	95:107	arg1	property					109:116	antibacterial property	95:116	antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection	95:187	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	6	64	theme	hemostasis	1047:1056	arg1	effect					1058:1063	a desirable hemostasis effect	1035:1063	a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg)	1035:1128	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	6	64	theme	hemostasis	1047:1056	arg1	time					1077:1080	hemostasis time	1066:1080	hemostasis time	1066:1080	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	0	65	theme	noncompressible	128:142	arg1	hemorrhage					144:153	rapid noncompressible hemorrhage	122:153	rapid noncompressible hemorrhage	122:153	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	9	66	theme	great	1585:1589	arg1	potential					1591:1599	great potential	1585:1599	great potential for clinical application as a multifunctional hemostatic agent	1585:1662	In summary, the shape memory sponges can quickly control bleeding and avoid bacterial infection, which shows great potential for clinical application as a multifunctional hemostatic agent.					
35839954	6	67	dep	time	1077:1080	arg1	285.25 ± 24.93 mg					1111:1127	285.25 ± 24.93 mg	1111:1127	285.25 ± 24.93 mg	1111:1127	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	6	67	dep	time	1077:1080	arg1	22.75 ± 3.86 s					1083:1096	22.75 ± 3.86 s	1083:1096	22.75 ± 3.86 s	1083:1096	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	6	67	dep	time	1077:1080	arg1	loss					1105:1108	blood loss	1099:1108	blood loss	1099:1108	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	8	68	theme	@	1377:1377	arg1	sponges					1381:1387	the EHP@Ag sponges	1370:1387	the EHP@Ag sponges	1370:1387	Furthermore, the antibacterial experiment in vivo showed that the EHP@Ag sponges could kill bacteria effectively and reduce the bacteria-induced inflammatory response.					
35839954	3	69	theme	cellulose/soy	502:514	arg1	EHSS					556:559	EHSS	556:559	EHSS	556:559	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	69	theme	cellulose/soy	502:514	arg1	sponge					548:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge	462:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection	462:631	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	5	70	theme	rapid	834:838	arg1	recovery					862:869	rapid blood-triggered shape recovery	834:869	rapid blood-triggered shape recovery	834:869	The EHP@Ag sponges showed rapid blood-triggered shape recovery speed, which is beneficial for administering noncompressible hemorrhage.					
35839954	3	71	theme	memory	482:487	arg1	EHSS					556:559	EHSS	556:559	EHSS	556:559	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	71	theme	memory	482:487	arg1	sponge					548:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge	462:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection	462:631	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	0	72	theme	hydroxyethyl	24:35	arg1	isolate					59:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate	0:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate	0:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	6	73	theme	blood	1099:1103	arg1	loss					1105:1108	blood loss	1099:1108	blood loss	1099:1108	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	3	74	theme	composite	538:546	arg1	EHSS					556:559	EHSS	556:559	EHSS	556:559	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	74	theme	composite	538:546	arg1	sponge					548:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge	462:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection	462:631	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	5	75	theme	@	815:815	arg1	sponges					819:825	The EHP@Ag sponges	808:825	The EHP@Ag sponges	808:825	The EHP@Ag sponges showed rapid blood-triggered shape recovery speed, which is beneficial for administering noncompressible hemorrhage.					
35839954	3	76	theme	injectable	465:474	arg1	EHSS					556:559	EHSS	556:559	EHSS	556:559	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	76	theme	injectable	465:474	arg1	sponge					548:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge	462:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection	462:631	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	77	theme	isolate	524:530	arg1	EHSS					556:559	EHSS	556:559	EHSS	556:559	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	3	77	theme	isolate	524:530	arg1	sponge					548:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge	462:553	an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection	462:631	Here, we prepared an injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge (EHSS) for rapid noncompressible hemorrhage and prevention of wound infection.					
35839954	9	78	theme	hemostatic	1647:1656	arg1	agent					1658:1662	a multifunctional hemostatic agent	1629:1662	a multifunctional hemostatic agent	1629:1662	In summary, the shape memory sponges can quickly control bleeding and avoid bacterial infection, which shows great potential for clinical application as a multifunctional hemostatic agent.					
35839954	6	79	theme	hemostasis	1173:1182	arg1	sponge					1165:1170	the commercial gelatin sponge	1142:1170	the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg)	1142:1235	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	6	79	theme	hemostasis	1173:1182	arg1	time					1184:1187	hemostasis time	1173:1187	hemostasis time	1173:1187	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	7	80	theme	antibacterial	1284:1296	arg1	property					1298:1305	an efficient antibacterial property	1271:1305	an efficient antibacterial property	1271:1305	Meanwhile, the EHP@Ag sponge has an efficient antibacterial property.					
35839954	4	81	theme	shape	665:669	arg1	Ag					690:691	EHP@Ag	686:691	EHP@Ag	686:691	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	4	81	theme	shape	665:669	arg1	sponge					678:683	The nano silver (AgNPs)-loaded shape memory sponge	634:683	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag)	634:692	The nano silver (AgNPs)-loaded shape memory sponge (EHP@Ag) was fabricated by mussel-inspired polydopamine coating EHSS sponge, then reducing and immobilizing AgNPs in situ.					
35839954	8	82	theme	bacteria-induced	1436:1451	arg1	response					1466:1473	the bacteria-induced inflammatory response	1432:1473	the bacteria-induced inflammatory response	1432:1473	Furthermore, the antibacterial experiment in vivo showed that the EHP@Ag sponges could kill bacteria effectively and reduce the bacteria-induced inflammatory response.					
35839954	2	83	theme	subsequent	416:425	arg1	infection					433:441	subsequent wound infection	416:441	subsequent wound infection	416:441	However, traditional hemostatic materials are ineffective for extreme bleeding and subsequent wound infection.					
35839954	6	84	theme	commercial	1146:1155	arg1	sponge					1165:1170	the commercial gelatin sponge	1142:1170	the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg)	1142:1235	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	6	84	theme	commercial	1146:1155	arg1	time					1184:1187	hemostasis time	1173:1187	hemostasis time	1173:1187	The results of the hemostatic experiment in vivo demonstrated that EHP@Ag sponge exhibited a desirable hemostasis effect (hemostasis time: 22.75 ± 3.86 s, blood loss: 285.25 ± 24.93 mg) compared to the commercial gelatin sponge (hemostasis time: 49.25 ± 3.30 s, blood loss: 755.50 ± 24.45 mg).					
35839954	0	85	theme	shape	11:15	arg1	isolate					59:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate	0:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate	0:65	Injectable shape memory hydroxyethyl cellulose/soy protein isolate based composite sponge with antibacterial property for rapid noncompressible hemorrhage and prevention of wound infection.					
37000307	2	0	theme	chemical	484:491	arg1	coagulants					505:514	chemical and natural coagulants	484:514	chemical and natural coagulants	484:514	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	0	1	from	optimization	13:24	arg1	water					92:96	water	92:96	water	92:96	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	0	1	from	optimization	13:24	arg1	removal					79:85	turbidity removal	69:85	turbidity removal from water	69:96	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	6	2	theme	proposed	1150:1157	arg1	model					1159:1163	the proposed model	1146:1163	the proposed model (quadratic model)	1146:1181	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	6	2	theme	proposed	1150:1157	arg1	model					1176:1180	quadratic model	1166:1180	quadratic model	1166:1180	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	6	3	theme	model	1159:1163	arg1	validity					1121:1128	validity	1121:1128	validity	1121:1128	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	6	3	theme	model	1159:1163	arg1	adequacy					1134:1141	adequacy	1134:1141	adequacy	1134:1141	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	4	4	theme	coagulant	844:852	arg1	dose					854:857	coagulant dose	844:857	coagulant dose (0-10 mg/L)	844:869	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	4	theme	coagulant	844:852	arg1	0-10 mg/L					860:868	0-10 mg/L	860:868	0-10 mg/L	860:868	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	4	theme	coagulant	844:852	arg1	factors					835:841	the four main factors	821:841	the four main factors	821:841	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	2	5	from	effects	554:560	arg1	water					588:592	water	588:592	water	588:592	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	3	6	theme	chemical	663:670	arg1	coagulant					672:680	a chemical coagulant and rice starch	661:696	coagulant	672:680	In this study, the application of poly aluminum chloride (PAC) as a chemical coagulant and rice starch as a natural coagulant aid to remove turbidity from aqueous solutions was investigated.					
37000307	2	7	from	coagulants	333:342	arg1	water					347:351	water	347:351	water	347:351	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	4	8	theme	main	830:833	arg1	turbidity					920:928	turbidity	920:928	turbidity (NTU 0-50)	920:939	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	8	theme	main	830:833	arg1	pH					910:911	pH	910:911	pH (5-9)	910:917	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	8	theme	main	830:833	arg1	dose					854:857	coagulant dose	844:857	coagulant dose (0-10 mg/L)	844:869	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	8	theme	main	830:833	arg1	dose					891:894	coagulant adjuvant dose	872:894	coagulant adjuvant dose (0-0.1 mg/L)	872:907	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	8	theme	main	830:833	arg1	levels					956:961	each five levels	946:961	each five levels	946:961	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	8	theme	main	830:833	arg1	factors					835:841	the four main factors	821:841	the four main factors	821:841	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	6	9	theme	fit	1286:1288	arg1	p-values					1254:1261	p-values	1254:1261	p-values of 0.0001	1254:1271	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	6	9	theme	fit	1286:1288	arg1	F-value					1237:1243	F-value	1237:1243	F-value of 23.3	1237:1251	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	6	9	theme	fit	1286:1288	arg1	lack					1278:1281	lack	1278:1281	lack of fit of 0.877 for the model	1278:1311	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	0	10	theme	poly	104:107	arg1	starch					136:141	rice starch	131:141	rice starch	131:141	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	0	10	theme	poly	104:107	arg1	coagulant					156:164	coagulant	156:164	coagulant	156:164	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	0	10	theme	poly	104:107	arg1	chloride					118:125	poly aluminum chloride	104:125	poly aluminum chloride	104:125	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	0	11	from	Modeling	0:7	arg1	water					92:96	water	92:96	water	92:96	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	0	11	from	Modeling	0:7	arg1	removal					79:85	turbidity removal	69:85	turbidity removal from water	69:96	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	3	12	dep	coagulant	711:719	arg1	remove					728:733	remove	728:733	to remove turbidity from aqueous solutions	725:766	In this study, the application of poly aluminum chloride (PAC) as a chemical coagulant and rice starch as a natural coagulant aid to remove turbidity from aqueous solutions was investigated.					
37000307	3	12	dep	coagulant	711:719	arg1	aid					721:723	aid	721:723	aid	721:723	In this study, the application of poly aluminum chloride (PAC) as a chemical coagulant and rice starch as a natural coagulant aid to remove turbidity from aqueous solutions was investigated.					
37000307	4	13	theme	adjuvant	882:889	arg1	0-0.1 mg/L					897:906	0-0.1 mg/L	897:906	0-0.1 mg/L	897:906	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	13	theme	adjuvant	882:889	arg1	dose					891:894	coagulant adjuvant dose	872:894	coagulant adjuvant dose (0-0.1 mg/L)	872:907	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	13	theme	adjuvant	882:889	arg1	factors					835:841	the four main factors	821:841	the four main factors	821:841	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	3	14	theme	rice	686:689	arg1	starch					691:696	a chemical coagulant and rice starch	661:696	starch	691:696	In this study, the application of poly aluminum chloride (PAC) as a chemical coagulant and rice starch as a natural coagulant aid to remove turbidity from aqueous solutions was investigated.					
37000307	2	15	theme	coagulants	574:583	arg1	effects					554:560	the harmful effects	542:560	the harmful effects of chemical coagulants in water	542:592	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	6	16	theme	corresponding	1205:1217	arg1	statistics					1219:1228	the corresponding statistics	1201:1228	the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj	1201:1343	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	4	17	theme	above	801:805	arg1	coagulants					807:816	the above coagulants	797:816	the above coagulants	797:816	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	2	18	theme	natural	390:396	arg1	materials					398:406	natural materials	390:406	natural materials	390:406	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	0	19	theme	aluminum	109:116	arg1	starch					136:141	rice starch	131:141	rice starch	131:141	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	0	19	theme	aluminum	109:116	arg1	coagulant					156:164	coagulant	156:164	coagulant	156:164	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	0	19	theme	aluminum	109:116	arg1	chloride					118:125	poly aluminum chloride	104:125	poly aluminum chloride	104:125	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	5	20	theme	elimination	1071:1081	arg1	efficiency					1083:1092	the maximum turbidity elimination efficiency	1049:1092	the maximum turbidity elimination efficiency	1049:1092	Under the optimized conditions, the maximum turbidity elimination efficiency was found to be 96.6%.					
37000307	5	20	theme	elimination	1071:1081	arg1	%					1114:1114	96.6%	1110:1114	96.6%	1110:1114	Under the optimized conditions, the maximum turbidity elimination efficiency was found to be 96.6%.					
37000307	6	21	dep	validity	1121:1128	arg1	The					1117:1119	The	1117:1119	The	1117:1119	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	4	22	from	Effects	786:792	arg1	turbidity					920:928	turbidity	920:928	turbidity (NTU 0-50)	920:939	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	22	from	Effects	786:792	arg1	pH					910:911	pH	910:911	pH (5-9)	910:917	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	22	from	Effects	786:792	arg1	dose					854:857	coagulant dose	844:857	coagulant dose (0-10 mg/L)	844:869	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	22	from	Effects	786:792	arg1	dose					891:894	coagulant adjuvant dose	872:894	coagulant adjuvant dose (0-0.1 mg/L)	872:907	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	22	from	Effects	786:792	arg1	levels					956:961	each five levels	946:961	each five levels	946:961	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	22	from	Effects	786:792	arg1	factors					835:841	the four main factors	821:841	the four main factors	821:841	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	2	23	theme	effect	380:385	arg1	lack					361:364	the lack	357:364	the lack of sufficient effect of natural materials alone in removing turbidity for proper performance	357:457	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	2	23	theme	effect	380:385	arg1	coagulants					333:342	chemical coagulants	324:342	chemical coagulants in water	324:351	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	2	24	theme	proper	440:445	arg1	performance					447:457	proper performance	440:457	proper performance	440:457	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	3	25	theme	chloride	643:650	arg1	application					614:624	the application	610:624	the application of poly aluminum chloride (PAC) as a chemical coagulant and rice starch as a natural coagulant aid to remove turbidity from aqueous solutions	610:766	In this study, the application of poly aluminum chloride (PAC) as a chemical coagulant and rice starch as a natural coagulant aid to remove turbidity from aqueous solutions was investigated.					
37000307	4	26	theme	central	985:991	arg1	CCD					1011:1013	CCD	1011:1013	CCD	1011:1013	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	26	theme	central	985:991	arg1	design					1003:1008	a central composite design	983:1008	a central composite design (CCD)	983:1014	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	2	27	theme	sufficient	369:378	arg1	effect					380:385	sufficient effect	369:385	sufficient effect of natural materials alone	369:412	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	0	28	theme	rice	131:134	arg1	starch					136:141	rice starch	131:141	rice starch	131:141	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	0	28	theme	rice	131:134	arg1	coagulant					156:164	coagulant	156:164	coagulant	156:164	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	0	28	theme	rice	131:134	arg1	chloride					118:125	poly aluminum chloride	104:125	poly aluminum chloride	104:125	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	2	29	from	lack	361:364	arg1	water					347:351	water	347:351	water	347:351	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	6	30	theme	0.0001	1266:1271	arg1	p-values					1254:1261	p-values	1254:1261	p-values of 0.0001	1254:1271	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	6	30	theme	0.0001	1266:1271	arg1	F-value					1237:1243	F-value	1237:1243	F-value of 23.3	1237:1251	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	6	30	theme	0.0001	1266:1271	arg1	lack					1278:1281	lack	1278:1281	lack of fit of 0.877 for the model	1278:1311	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	5	31	theme	maximum	1053:1059	arg1	efficiency					1083:1092	the maximum turbidity elimination efficiency	1049:1092	the maximum turbidity elimination efficiency	1049:1092	Under the optimized conditions, the maximum turbidity elimination efficiency was found to be 96.6%.					
37000307	5	31	theme	maximum	1053:1059	arg1	%					1114:1114	96.6%	1110:1114	96.6%	1110:1114	Under the optimized conditions, the maximum turbidity elimination efficiency was found to be 96.6%.					
37000307	0	32	from	water	92:96	arg1	optimization					13:24	optimization	13:24	optimization	13:24	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	0	32	from	water	92:96	arg1	Modeling					0:7	Modeling	0:7	Modeling	0:7	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	0	32	from	water	92:96	arg1	removal					79:85	turbidity removal	69:85	turbidity removal from water	69:96	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	1	33	theme	coagulation/flocculation	194:217	arg1	process					219:225	the coagulation/flocculation process	190:225	the coagulation/flocculation process	190:225	The application of the coagulation/flocculation process is very important due to its simplicity in removing turbidity.					
37000307	0	34	theme	coagulation/flocculation	33:56	arg1	process					58:64	the coagulation/flocculation process	29:64	the coagulation/flocculation process	29:64	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	5	35	theme	turbidity	1061:1069	arg1	efficiency					1083:1092	the maximum turbidity elimination efficiency	1049:1092	the maximum turbidity elimination efficiency	1049:1092	Under the optimized conditions, the maximum turbidity elimination efficiency was found to be 96.6%.					
37000307	5	35	theme	turbidity	1061:1069	arg1	%					1114:1114	96.6%	1110:1114	96.6%	1110:1114	Under the optimized conditions, the maximum turbidity elimination efficiency was found to be 96.6%.					
37000307	4	36	theme	coagulant	872:880	arg1	0-0.1 mg/L					897:906	0-0.1 mg/L	897:906	0-0.1 mg/L	897:906	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	36	theme	coagulant	872:880	arg1	dose					891:894	coagulant adjuvant dose	872:894	coagulant adjuvant dose (0-0.1 mg/L)	872:907	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	36	theme	coagulant	872:880	arg1	factors					835:841	the four main factors	821:841	the four main factors	821:841	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	3	37	theme	poly	629:632	arg1	chloride					643:650	poly aluminum chloride	629:650	poly aluminum chloride (PAC)	629:656	In this study, the application of poly aluminum chloride (PAC) as a chemical coagulant and rice starch as a natural coagulant aid to remove turbidity from aqueous solutions was investigated.					
37000307	3	37	theme	poly	629:632	arg1	PAC					653:655	PAC	653:655	PAC	653:655	In this study, the application of poly aluminum chloride (PAC) as a chemical coagulant and rice starch as a natural coagulant aid to remove turbidity from aqueous solutions was investigated.					
37000307	4	38	theme	composite	993:1001	arg1	CCD					1011:1013	CCD	1011:1013	CCD	1011:1013	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	4	38	theme	composite	993:1001	arg1	design					1003:1008	a central composite design	983:1008	a central composite design (CCD)	983:1014	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	1	39	theme	process	219:225	arg1	application					175:185	The application	171:185	The application of the coagulation/flocculation process	171:225	The application of the coagulation/flocculation process is very important due to its simplicity in removing turbidity.					
37000307	1	39	theme	process	219:225	arg1	important					235:243	important	235:243	important	235:243	The application of the coagulation/flocculation process is very important due to its simplicity in removing turbidity.					
37000307	2	40	theme	simultaneous	464:475	arg1	way					528:530	the best way	519:530	the best way to reduce the harmful effects of chemical coagulants in water	519:592	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	2	40	theme	simultaneous	464:475	arg1	use					477:479	the simultaneous use	460:479	the simultaneous use of chemical and natural coagulants	460:514	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	2	41	theme	coagulants	505:514	arg1	way					528:530	the best way	519:530	the best way to reduce the harmful effects of chemical coagulants in water	519:592	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	2	41	theme	coagulants	505:514	arg1	use					477:479	the simultaneous use	460:479	the simultaneous use of chemical and natural coagulants	460:514	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	2	42	theme	best	523:526	arg1	way					528:530	the best way	519:530	the best way to reduce the harmful effects of chemical coagulants in water	519:592	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	2	42	theme	best	523:526	arg1	use					477:479	the simultaneous use	460:479	the simultaneous use of chemical and natural coagulants	460:514	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	2	43	theme	chemical	565:572	arg1	coagulants					574:583	chemical coagulants	565:583	chemical coagulants	565:583	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	4	44	theme	coagulants	807:816	arg1	Effects					786:792	Effects	786:792	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels	786:961	Effects of the above coagulants on the four main factors, coagulant dose (0-10 mg/L), coagulant adjuvant dose (0-0.1 mg/L), pH (5-9), turbidity (NTU 0-50), and each five levels were assessed using a central composite design (CCD).					
37000307	0	45	theme	process	58:64	arg1	optimization					13:24	optimization	13:24	optimization	13:24	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	0	45	theme	process	58:64	arg1	Modeling					0:7	Modeling	0:7	Modeling	0:7	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	5	46	theme	optimized	1027:1035	arg1	conditions					1037:1046	the optimized conditions	1023:1046	the optimized conditions	1023:1046	Under the optimized conditions, the maximum turbidity elimination efficiency was found to be 96.6%.					
37000307	6	47	theme	quadratic	1166:1174	arg1	model					1159:1163	the proposed model	1146:1163	the proposed model (quadratic model)	1146:1181	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	6	47	theme	quadratic	1166:1174	arg1	model					1176:1180	quadratic model	1166:1180	quadratic model	1166:1180	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	0	48	dep	coagulant	156:164	arg1	aid					166:168	aid	166:168	aid	166:168	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	6	49	dep	F-value	1237:1243	arg1	i.e.					1231:1234	i.e.	1231:1234	i.e.	1231:1234	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	2	50	theme	harmful	546:552	arg1	effects					554:560	the harmful effects	542:560	the harmful effects of chemical coagulants in water	542:592	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	3	51	theme	aluminum	634:641	arg1	chloride					643:650	poly aluminum chloride	629:650	poly aluminum chloride (PAC)	629:656	In this study, the application of poly aluminum chloride (PAC) as a chemical coagulant and rice starch as a natural coagulant aid to remove turbidity from aqueous solutions was investigated.					
37000307	3	51	theme	aluminum	634:641	arg1	PAC					653:655	PAC	653:655	PAC	653:655	In this study, the application of poly aluminum chloride (PAC) as a chemical coagulant and rice starch as a natural coagulant aid to remove turbidity from aqueous solutions was investigated.					
37000307	2	52	theme	chemical	324:331	arg1	coagulants					333:342	chemical coagulants	324:342	chemical coagulants in water	324:351	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	2	53	theme	natural	497:503	arg1	coagulants					505:514	chemical and natural coagulants	484:514	chemical and natural coagulants	484:514	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	2	54	theme	materials	398:406	arg1	effect					380:385	sufficient effect	369:385	sufficient effect of natural materials alone	369:412	Due to the disadvantages of using chemical coagulants in water and the lack of sufficient effect of natural materials alone in removing turbidity for proper performance, the simultaneous use of chemical and natural coagulants is the best way to reduce the harmful effects of chemical coagulants in water.					
37000307	6	55	theme	23.3	1248:1251	arg1	p-values					1254:1261	p-values	1254:1261	p-values of 0.0001	1254:1271	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	6	55	theme	23.3	1248:1251	arg1	F-value					1237:1243	F-value	1237:1243	F-value of 23.3	1237:1251	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	6	55	theme	23.3	1248:1251	arg1	lack					1278:1281	lack	1278:1281	lack of fit of 0.877 for the model	1278:1311	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	7	56	dep	= 0.84	1346:1351	arg1	AP = 22.04					1370:1379	AP = 22.04	1370:1379	AP = 22.04	1370:1379	= 0.84, R2 pred = 0.79, AP = 22.04).					
37000307	0	57	theme	turbidity	69:77	arg1	removal					79:85	turbidity removal	69:85	turbidity removal from water	69:96	Modeling and optimization of the coagulation/flocculation process in turbidity removal from water using poly aluminum chloride and rice starch as a natural coagulant aid.					
37000307	6	58	theme	0.877	1293:1297	arg1	p-values					1254:1261	p-values	1254:1261	p-values of 0.0001	1254:1271	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	6	58	theme	0.877	1293:1297	arg1	F-value					1237:1243	F-value	1237:1243	F-value of 23.3	1237:1251	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	6	58	theme	0.877	1293:1297	arg1	lack					1278:1281	lack	1278:1281	lack of fit of 0.877 for the model	1278:1311	The validity and adequacy of the proposed model (quadratic model) were confirmed by the corresponding statistics (i.e., F-value of 23.3, p-values of 0.0001, and lack of fit of 0.877 for the model, respectively, R2 = 0.88, R2adj.					
37000307	3	59	theme	aqueous	750:756	arg1	solutions					758:766	aqueous solutions	750:766	aqueous solutions	750:766	In this study, the application of poly aluminum chloride (PAC) as a chemical coagulant and rice starch as a natural coagulant aid to remove turbidity from aqueous solutions was investigated.					
36662189	6	0	theme	chronic	1057:1063	arg1	colitis					1065:1071	DSS-induced chronic colitis	1045:1071	DSS-induced chronic colitis in mice	1045:1079	In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice.					
36662189	8	1	theme	IBD	1593:1595	arg1	treatment					1597:1605	IBD treatment	1593:1605	IBD treatment	1593:1605	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	7	2	from	phosphorylation	1200:1214	arg1	tissues					1229:1235	the colon tissues	1219:1235	the colon tissues	1219:1235	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	2	3	dep	METHODS	314:320	arg1	polysaccharide					336:349	A polysaccharide	334:349	METHODS AND RESULTS A polysaccharide named Fuc-S	314:361	METHODS AND RESULTS A polysaccharide named Fuc-S, with a molecular weight of 156 kDa, was prepared by the ultrasonic degradation of fucoidan.					
36662189	1	4	theme	pathogenic	248:257	arg1	role					259:262	an important pathogenic role	235:262	an important pathogenic role	235:262	SCOPE The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease.					
36662189	7	5	from	tissues	1229:1235	arg1	inhibition					1162:1171	the inhibition	1158:1171	the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis	1158:1385	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	6	6	dep	In	873:874	arg1	vivo					876:879	vivo	876:879	vivo	876:879	In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice.					
36662189	7	7	theme	host-microbe	1319:1330	arg1	metabolism					1343:1352	the host-microbe tryptophan metabolism	1315:1352	the host-microbe tryptophan metabolism of the mice with chronic colitis	1315:1385	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	5	8	theme	longitudinal	791:802	arg1	alterations					815:825	The longitudinal microbiome alterations	787:825	The longitudinal microbiome alterations	787:825	The longitudinal microbiome alterations were evaluated using 16S amplicon sequencing.					
36662189	7	9	from	Akt	1176:1178	arg1	tissues					1229:1235	the colon tissues	1219:1235	the colon tissues	1219:1235	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	5	10	theme	amplicon	852:859	arg1	sequencing					861:870	16S amplicon sequencing	848:870	16S amplicon sequencing	848:870	The longitudinal microbiome alterations were evaluated using 16S amplicon sequencing.					
36662189	6	11	theme	In	873:874	arg1	assays					881:886	In vivo assays	873:886	In vivo assays	873:886	In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice.					
36662189	7	12	from	p-38	1181:1184	arg1	tissues					1229:1235	the colon tissues	1219:1235	the colon tissues	1219:1235	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	1	13	theme	The	188:190	arg1	dysbiosis					192:200	SCOPE The dysbiosis	182:200	SCOPE The dysbiosis of intestinal microecology	182:227	SCOPE The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease.					
36662189	6	14	theme	colon	972:976	arg1	injury					978:983	colon injury	972:983	colon injury	972:983	In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice.					
36662189	7	15	from	ERK	1187:1189	arg1	tissues					1229:1235	the colon tissues	1219:1235	the colon tissues	1219:1235	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	7	16	theme	gut	1284:1286	arg1	microbiota					1288:1297	the gut microbiota	1280:1297	the gut microbiota	1280:1297	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	4	17	theme	C57BL/6	659:665	arg1	mice					667:670	male C57BL/6 mice	654:670	male C57BL/6 mice	654:670	Moreover, male C57BL/6 mice were fed three cycles of 1.8% dextran sulfate sodium (DSS) for 5 days and then water for 7 days to induce colitis.					
36662189	7	18	theme	ERK	1187:1189	arg1	inhibition					1162:1171	the inhibition	1158:1171	the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis	1158:1385	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	0	19	theme	Ultrasonic	12:21	arg1	α-l-Fucooligosaccharide-Alleviates					41:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates	0:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates	0:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host-Microbe Tryptophan Metabolism.					
36662189	0	20	theme	Gut	116:118	arg1	Microbiota					120:129	Gut Microbiota	116:129	Gut Microbiota	116:129	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host-Microbe Tryptophan Metabolism.					
36662189	1	21	theme	microecology	216:227	arg1	dysbiosis					192:200	SCOPE The dysbiosis	182:200	SCOPE The dysbiosis of intestinal microecology	182:227	SCOPE The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease.					
36662189	7	22	theme	Akt	1176:1178	arg1	inhibition					1162:1171	the inhibition	1158:1171	the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis	1158:1385	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	6	23	theme	clinical	929:936	arg1	manifestations					938:951	clinical manifestations	929:951	clinical manifestations	929:951	In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice.					
36662189	8	24	theme	fucoidan	1461:1468	arg1	backbone					1449:1456	the backbone	1445:1456	the backbone of fucoidan	1445:1468	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	0	25	theme	Host-Microbe	146:157	arg1	Metabolism					170:179	Host-Microbe Tryptophan Metabolism	146:179	Host-Microbe Tryptophan Metabolism	146:179	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host-Microbe Tryptophan Metabolism.					
36662189	7	26	dep	tissues	1229:1235	arg1	regulating					1238:1247	regulating	1238:1247	regulating the structure and abundance of the gut microbiota	1238:1297	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	7	26	dep	tissues	1229:1235	arg1	modulating					1304:1313	modulating	1304:1313	modulating the host-microbe tryptophan metabolism of the mice with chronic colitis	1304:1385	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	4	27	theme	%	700:700	arg1	DSS					726:728	1.8% dextran sulfate sodium (DSS)	697:729	1.8% dextran sulfate sodium (DSS)	697:729	Moreover, male C57BL/6 mice were fed three cycles of 1.8% dextran sulfate sodium (DSS) for 5 days and then water for 7 days to induce colitis.					
36662189	2	28	theme	ultrasonic	420:429	arg1	degradation					431:441	the ultrasonic degradation	416:441	the ultrasonic degradation of fucoidan	416:453	METHODS AND RESULTS A polysaccharide named Fuc-S, with a molecular weight of 156 kDa, was prepared by the ultrasonic degradation of fucoidan.					
36662189	0	29	theme	Fuc-S-A	0:6	arg1	α-l-Fucooligosaccharide-Alleviates					41:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates	0:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates	0:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host-Microbe Tryptophan Metabolism.					
36662189	6	30	theme	cell	1011:1014	arg1	infiltration					1016:1027	colonic inflammatory cell infiltration	990:1027	colonic inflammatory cell infiltration	990:1027	In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice.					
36662189	4	31	theme	sulfate	710:716	arg1	DSS					726:728	1.8% dextran sulfate sodium (DSS)	697:729	1.8% dextran sulfate sodium (DSS)	697:729	Moreover, male C57BL/6 mice were fed three cycles of 1.8% dextran sulfate sodium (DSS) for 5 days and then water for 7 days to induce colitis.					
36662189	3	32	theme	NMR	507:509	arg1	analysis					520:527	NMR spectral analysis	507:527	NMR spectral analysis	507:527	Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→.					
36662189	7	33	with	mice	1361:1364	arg1	colitis					1379:1385	chronic colitis	1371:1385	chronic colitis	1371:1385	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	6	34	theme	colonic	990:996	arg1	infiltration					1016:1027	colonic inflammatory cell infiltration	990:1027	colonic inflammatory cell infiltration	990:1027	In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice.					
36662189	3	35	theme	Monosaccharide	456:469	arg1	composition					471:481	Monosaccharide composition	456:481	Monosaccharide composition	456:481	Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→.					
36662189	7	36	theme	beneficial	1118:1127	arg1	effects					1129:1135	these beneficial effects	1112:1135	these beneficial effects	1112:1135	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	0	37	theme	Sulfated	32:39	arg1	α-l-Fucooligosaccharide-Alleviates					41:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates	0:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates	0:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host-Microbe Tryptophan Metabolism.					
36662189	2	38	theme	kDa	395:397	arg1	weight					381:386	a molecular weight	369:386	a molecular weight of 156 kDa	369:397	METHODS AND RESULTS A polysaccharide named Fuc-S, with a molecular weight of 156 kDa, was prepared by the ultrasonic degradation of fucoidan.					
36662189	8	39	theme	effective	1534:1542	arg1	Fuc-S					1507:1511	Fuc-S	1507:1511	Fuc-S	1507:1511	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	8	39	theme	effective	1534:1542	arg1	candidate					1579:1587	an effective functional food and pharmaceutical candidate	1531:1587	an effective functional food and pharmaceutical candidate for IBD treatment	1531:1605	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	1	40	theme	bowel	299:303	arg1	disease					305:311	inflammatory bowel disease	286:311	inflammatory bowel disease	286:311	SCOPE The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease.					
36662189	7	41	dep	structure	1253:1261	arg1	the					1249:1251	the	1249:1251	the	1249:1251	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	7	42	theme	phosphorylation	1200:1214	arg1	inhibition					1162:1171	the inhibition	1158:1171	the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis	1158:1385	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	8	43	theme	food	1555:1558	arg1	Fuc-S					1507:1511	Fuc-S	1507:1511	Fuc-S	1507:1511	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	8	43	theme	food	1555:1558	arg1	candidate					1579:1587	an effective functional food and pharmaceutical candidate	1531:1587	an effective functional food and pharmaceutical candidate for IBD treatment	1531:1605	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	7	44	theme	Further	1082:1088	arg1	studies					1090:1096	Further studies	1082:1096	Further studies	1082:1096	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	6	45	from	colitis	1065:1071	arg1	mice					1076:1079	mice	1076:1079	mice	1076:1079	In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice.					
36662189	8	46	attach	presence	1422:1429	arg2	glucose					1434:1440	glucose	1434:1440	glucose	1434:1440	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	8	46	attach	presence	1422:1429	arg1	backbone					1449:1456	the backbone	1445:1456	the backbone of fucoidan	1445:1468	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	6	47	theme	DSS-induced	1045:1055	arg1	colitis					1065:1071	DSS-induced chronic colitis	1045:1071	DSS-induced chronic colitis in mice	1045:1079	In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice.					
36662189	8	48	theme	pharmaceutical	1564:1577	arg1	Fuc-S					1507:1511	Fuc-S	1507:1511	Fuc-S	1507:1511	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	8	48	theme	pharmaceutical	1564:1577	arg1	candidate					1579:1587	an effective functional food and pharmaceutical candidate	1531:1587	an effective functional food and pharmaceutical candidate for IBD treatment	1531:1605	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	2	49	theme	molecular	371:379	arg1	weight					381:386	a molecular weight	369:386	a molecular weight of 156 kDa	369:397	METHODS AND RESULTS A polysaccharide named Fuc-S, with a molecular weight of 156 kDa, was prepared by the ultrasonic degradation of fucoidan.					
36662189	1	50	theme	important	238:246	arg1	role					259:262	an important pathogenic role	235:262	an important pathogenic role	235:262	SCOPE The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease.					
36662189	7	51	theme	chronic	1371:1377	arg1	colitis					1379:1385	chronic colitis	1371:1385	chronic colitis	1371:1385	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	7	52	theme	p-38	1181:1184	arg1	inhibition					1162:1171	the inhibition	1158:1171	the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis	1158:1385	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	3	53	theme	1→	598:599	arg1	-α-L-Fucp-					605:614	→3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→	584:641	-α-L-Fucp-	605:614	Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→.					
36662189	3	54	dep	-α-L-Fucp-	605:614	arg1	→4					602:603	→3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→	584:641	→4	602:603	Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→.					
36662189	7	55	theme	mice	1361:1364	arg1	metabolism					1343:1352	the host-microbe tryptophan metabolism	1315:1352	the host-microbe tryptophan metabolism of the mice with chronic colitis	1315:1385	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	3	56	theme	-α-L-Fucp-	587:596	arg1	-α-L-Fucp-					605:614	→3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→	584:641	-α-L-Fucp-	605:614	Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→.					
36662189	5	57	theme	microbiome	804:813	arg1	alterations					815:825	The longitudinal microbiome alterations	787:825	The longitudinal microbiome alterations	787:825	The longitudinal microbiome alterations were evaluated using 16S amplicon sequencing.					
36662189	2	58	with	METHODS	314:320	arg1	weight					381:386	a molecular weight	369:386	a molecular weight of 156 kDa	369:397	METHODS AND RESULTS A polysaccharide named Fuc-S, with a molecular weight of 156 kDa, was prepared by the ultrasonic degradation of fucoidan.					
36662189	7	59	theme	tryptophan	1332:1341	arg1	metabolism					1343:1352	the host-microbe tryptophan metabolism	1315:1352	the host-microbe tryptophan metabolism of the mice with chronic colitis	1315:1385	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	5	60	theme	16S	848:850	arg1	sequencing					861:870	16S amplicon sequencing	848:870	16S amplicon sequencing	848:870	The longitudinal microbiome alterations were evaluated using 16S amplicon sequencing.					
36662189	2	61	with	RESULTS	326:332	arg1	weight					381:386	a molecular weight	369:386	a molecular weight of 156 kDa	369:397	METHODS AND RESULTS A polysaccharide named Fuc-S, with a molecular weight of 156 kDa, was prepared by the ultrasonic degradation of fucoidan.					
36662189	1	62	theme	SCOPE	182:186	arg1	dysbiosis					192:200	SCOPE The dysbiosis	182:200	SCOPE The dysbiosis of intestinal microecology	182:227	SCOPE The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease.					
36662189	8	63	theme	glucose	1434:1440	arg1	presence					1422:1429	the presence	1418:1429	the presence of glucose in the backbone of fucoidan	1418:1468	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	3	64	contain	have	554:557	arg2	backbone					561:568	a backbone	559:568	a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→	559:641	Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→.					
36662189	3	64	contain	have	554:557	arg1	Fuc-S					544:548	Fuc-S	544:548	Fuc-S	544:548	Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→.					
36662189	7	65	theme	microbiota	1288:1297	arg1	abundance					1267:1275	abundance	1267:1275	abundance	1267:1275	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	7	65	theme	microbiota	1288:1297	arg1	structure					1253:1261	structure	1253:1261	structure	1253:1261	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	4	66	theme	male	654:657	arg1	mice					667:670	male C57BL/6 mice	654:670	male C57BL/6 mice	654:670	Moreover, male C57BL/6 mice were fed three cycles of 1.8% dextran sulfate sodium (DSS) for 5 days and then water for 7 days to induce colitis.					
36662189	1	67	theme	intestinal	205:214	arg1	microecology					216:227	intestinal microecology	205:227	intestinal microecology	205:227	SCOPE The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease.					
36662189	6	68	theme	colon	954:958	arg1	shortening					960:969	colon shortening	954:969	colon shortening	954:969	In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice.					
36662189	4	69	theme	sodium	718:723	arg1	DSS					726:728	1.8% dextran sulfate sodium (DSS)	697:729	1.8% dextran sulfate sodium (DSS)	697:729	Moreover, male C57BL/6 mice were fed three cycles of 1.8% dextran sulfate sodium (DSS) for 5 days and then water for 7 days to induce colitis.					
36662189	0	70	theme	Tryptophan	159:168	arg1	Metabolism					170:179	Host-Microbe Tryptophan Metabolism	146:179	Host-Microbe Tryptophan Metabolism	146:179	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host-Microbe Tryptophan Metabolism.					
36662189	8	71	theme	useful	1483:1488	arg1	information					1490:1500	useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment	1483:1605	useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment	1483:1605	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	4	72	theme	DSS	726:728	arg1	cycles					687:692	three cycles	681:692	three cycles of 1.8% dextran sulfate sodium (DSS)	681:729	Moreover, male C57BL/6 mice were fed three cycles of 1.8% dextran sulfate sodium (DSS) for 5 days and then water for 7 days to induce colitis.					
36662189	8	73	from	presence	1422:1429	arg1	backbone					1449:1456	the backbone	1445:1456	the backbone of fucoidan	1445:1468	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	0	74	theme	New	8:10	arg1	α-l-Fucooligosaccharide-Alleviates					41:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates	0:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates	0:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host-Microbe Tryptophan Metabolism.					
36662189	4	75	theme	1.8	697:699	arg1	%					700:700	%	700:700	%	700:700	Moreover, male C57BL/6 mice were fed three cycles of 1.8% dextran sulfate sodium (DSS) for 5 days and then water for 7 days to induce colitis.					
36662189	7	76	from	inhibition	1162:1171	arg1	tissues					1229:1235	the colon tissues	1219:1235	the colon tissues	1219:1235	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	0	77	theme	Degraded	23:30	arg1	α-l-Fucooligosaccharide-Alleviates					41:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates	0:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates	0:74	Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host-Microbe Tryptophan Metabolism.					
36662189	6	78	theme	inflammatory	998:1009	arg1	infiltration					1016:1027	colonic inflammatory cell infiltration	990:1027	colonic inflammatory cell infiltration	990:1027	In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice.					
36662189	3	79	theme	-α-D-Glcp-	629:638	arg1	1→					640:641	→3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→	584:641	1→	640:641	Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→.					
36662189	4	80	theme	dextran	702:708	arg1	DSS					726:728	1.8% dextran sulfate sodium (DSS)	697:729	1.8% dextran sulfate sodium (DSS)	697:729	Moreover, male C57BL/6 mice were fed three cycles of 1.8% dextran sulfate sodium (DSS) for 5 days and then water for 7 days to induce colitis.					
36662189	3	81	theme	spectral	511:518	arg1	analysis					520:527	NMR spectral analysis	507:527	NMR spectral analysis	507:527	Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→.					
36662189	2	82	theme	fucoidan	446:453	arg1	degradation					431:441	the ultrasonic degradation	416:441	the ultrasonic degradation of fucoidan	416:453	METHODS AND RESULTS A polysaccharide named Fuc-S, with a molecular weight of 156 kDa, was prepared by the ultrasonic degradation of fucoidan.					
36662189	7	83	theme	colon	1223:1227	arg1	tissues					1229:1235	the colon tissues	1219:1235	the colon tissues	1219:1235	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	8	84	dep	CONCLUSION	1388:1397	arg1	confirmed					1408:1416	confirmed	1408:1416	confirmed the presence of glucose in the backbone of fucoidan	1408:1468	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	8	84	dep	CONCLUSION	1388:1397	arg1	provided					1474:1481	provided	1474:1481	provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment	1474:1605	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	8	85	theme	functional	1544:1553	arg1	Fuc-S					1507:1511	Fuc-S	1507:1511	Fuc-S	1507:1511	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	8	85	theme	functional	1544:1553	arg1	candidate					1579:1587	an effective functional food and pharmaceutical candidate	1531:1587	an effective functional food and pharmaceutical candidate for IBD treatment	1531:1605	CONCLUSION Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.					
36662189	1	86	theme	inflammatory	286:297	arg1	disease					305:311	inflammatory bowel disease	286:311	inflammatory bowel disease	286:311	SCOPE The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease.					
36662189	7	87	theme	JNK	1196:1198	arg1	phosphorylation					1200:1214	JNK phosphorylation	1196:1214	JNK phosphorylation	1196:1214	Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.					
36662189	1	88	theme	disease	305:311	arg1	development					271:281	the development	267:281	the development of inflammatory bowel disease	267:311	SCOPE The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease.					
36696220	2	0	theme	various	346:352	arg1	constituents					354:365	various constituents	346:365	various constituents of venous thrombi	346:383	Further, although various constituents of venous thrombi have been identified, their localizations and cellular and molecular interactions are yet to be combined in a single, multiplexed analysis.					
36696220	8	1	located	localized	1438:1446	arg1	thrombus					1461:1468	the white thrombus	1451:1468	the white thrombus regardless of endotoxemia	1451:1494	Our detailed structural analysis also showed that most thrombus components are localized in the white thrombus regardless of endotoxemia.					
36696220	8	1	located	localized	1438:1446	arg2	components					1423:1432	most thrombus components	1409:1432	most thrombus components	1409:1432	Our detailed structural analysis also showed that most thrombus components are localized in the white thrombus regardless of endotoxemia.					
36696220	3	2	dep	OBJECTIVES	525:534	arg1	was					564:566	was	564:566	was to investigate the role of the von Willebrand factor (VWF) in inflammation-associated venous thrombosis	564:670	OBJECTIVES The objective of this study was to investigate the role of the von Willebrand factor (VWF) in inflammation-associated venous thrombosis.					
36696220	7	3	contain	had	1215:1217	arg2	incidences					1240:1249	significantly higher incidences	1219:1249	significantly higher incidences of venous thrombosis	1219:1270	RESULTS We found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies.					
36696220	7	3	contain	had	1215:1217	arg2	effect					1276:1281	an effect	1273:1281	an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies	1273:1356	RESULTS We found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies.					
36696220	7	3	contain	had	1215:1217	arg1	mice					1210:1213	lipopolysaccharide-treated mice	1183:1213	lipopolysaccharide-treated mice	1183:1213	RESULTS We found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies.					
36696220	10	4	theme	thrombosis	1833:1842	arg1	pathogenesis					1786:1797	the pathogenesis	1782:1797	the pathogenesis of inflammation-associated venous thrombosis	1782:1842	CONCLUSIONS We observe a key role for VWF in the pathogenesis of inflammation-associated venous thrombosis while providing a more comprehensive insight into the molecular interactions that constitute the architecture of venous thrombi.					
36696220	5	5	theme	murine	882:887	arg1	model					917:921	the murine inferior vena cava stenosis model	878:921	the murine inferior vena cava stenosis model of deep vein thrombosis	878:945	METHODS We combined the murine inferior vena cava stenosis model of deep vein thrombosis with the lipopolysaccharide model of endotoxemia.					
36696220	4	6	dep	localizations	791:803	arg1	the					787:789	the	787:789	the	787:789	We also performed a proof-of-concept study of imaging mass cytometry to quantitatively and simultaneously analyze the localizations and interactions of 10 venous thrombus constituents.					
36696220	7	7	dep	RESULTS	1161:1167	arg1	found					1172:1176	found	1172:1176	found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies	1172:1356	RESULTS We found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies.					
36696220	3	8	theme	study	558:562	arg1	objective					540:548	The objective	536:548	The objective of this study	536:562	OBJECTIVES The objective of this study was to investigate the role of the von Willebrand factor (VWF) in inflammation-associated venous thrombosis.					
36696220	9	9	theme	relative	1542:1549	arg1	representation					1551:1564	the relative representation	1538:1564	the relative representation	1538:1564	Moreover, although endotoxemia modulated the relative representation and interactions of VWF with other thrombus constituents, the scaffolding network, comprised VWF, fibrin, and neutrophil extracellular traps, remained largely unaffected.					
36696220	5	10	theme	vein	931:934	arg1	thrombosis					936:945	deep vein thrombosis	926:945	deep vein thrombosis	926:945	METHODS We combined the murine inferior vena cava stenosis model of deep vein thrombosis with the lipopolysaccharide model of endotoxemia.					
36696220	4	11	theme	imaging	719:725	arg1	cytometry					732:740	imaging mass cytometry	719:740	imaging mass cytometry	719:740	We also performed a proof-of-concept study of imaging mass cytometry to quantitatively and simultaneously analyze the localizations and interactions of 10 venous thrombus constituents.					
36696220	1	12	from	role	231:234	arg1	pathogenesis					259:270	the pathogenesis	255:270	the pathogenesis of deep vein thrombosis	255:294	BACKGROUND Although the concept of immunothrombosis has established a link between inflammation and thrombosis, the role of inflammation in the pathogenesis of deep vein thrombosis remains to be fully elucidated.					
36696220	3	13	theme	factor	614:619	arg1	role					587:590	the role	583:590	the role of the von Willebrand factor (VWF) in inflammation-associated venous thrombosis	583:670	OBJECTIVES The objective of this study was to investigate the role of the von Willebrand factor (VWF) in inflammation-associated venous thrombosis.					
36696220	6	14	theme	mass	1051:1054	arg1	cytometry					1056:1064	imaging mass cytometry	1043:1064	imaging mass cytometry	1043:1064	We also performed a proof-of-concept study of imaging mass cytometry to assess the feasibility of this approach in analyzing the structural composition of thrombi.					
36696220	2	15	theme	single	495:500	arg1	analysis					515:522	a single, multiplexed analysis	493:522	analysis	515:522	Further, although various constituents of venous thrombi have been identified, their localizations and cellular and molecular interactions are yet to be combined in a single, multiplexed analysis.					
36696220	8	16	theme	structural	1372:1381	arg1	analysis					1383:1390	Our detailed structural analysis	1359:1390	Our detailed structural analysis	1359:1390	Our detailed structural analysis also showed that most thrombus components are localized in the white thrombus regardless of endotoxemia.					
36696220	0	17	theme	factor	54:59	arg1	role					31:34	The mechanistic and structural role	0:34	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.	0:113	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.					
36696220	3	18	theme	von	599:601	arg1	VWF					622:624	VWF	622:624	VWF	622:624	OBJECTIVES The objective of this study was to investigate the role of the von Willebrand factor (VWF) in inflammation-associated venous thrombosis.					
36696220	3	18	theme	von	599:601	arg1	factor					614:619	von Willebrand factor	599:619	the von Willebrand factor (VWF)	595:625	OBJECTIVES The objective of this study was to investigate the role of the von Willebrand factor (VWF) in inflammation-associated venous thrombosis.					
36696220	9	19	theme	thrombus	1601:1608	arg1	constituents					1610:1621	other thrombus constituents	1595:1621	other thrombus constituents	1595:1621	Moreover, although endotoxemia modulated the relative representation and interactions of VWF with other thrombus constituents, the scaffolding network, comprised VWF, fibrin, and neutrophil extracellular traps, remained largely unaffected.					
36696220	0	20	theme	endotoxemia-enhanced	64:83	arg1	thrombosis					95:104	endotoxemia-enhanced deep vein thrombosis	64:104	endotoxemia-enhanced deep vein thrombosis in mice	64:112	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.					
36696220	6	21	theme	proof-of-concept	1017:1032	arg1	study					1034:1038	a proof-of-concept study	1015:1038	a proof-of-concept study of imaging mass cytometry	1015:1064	We also performed a proof-of-concept study of imaging mass cytometry to assess the feasibility of this approach in analyzing the structural composition of thrombi.					
36696220	0	22	theme	vein	90:93	arg1	thrombosis					95:104	endotoxemia-enhanced deep vein thrombosis	64:104	endotoxemia-enhanced deep vein thrombosis in mice	64:112	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.					
36696220	9	23	theme	scaffolding	1628:1638	arg1	network					1640:1646	the scaffolding network	1624:1646	the scaffolding network	1624:1646	Moreover, although endotoxemia modulated the relative representation and interactions of VWF with other thrombus constituents, the scaffolding network, comprised VWF, fibrin, and neutrophil extracellular traps, remained largely unaffected.					
36696220	2	24	theme	multiplexed	503:513	arg1	analysis					515:522	a single, multiplexed analysis	493:522	analysis	515:522	Further, although various constituents of venous thrombi have been identified, their localizations and cellular and molecular interactions are yet to be combined in a single, multiplexed analysis.					
36696220	8	25	theme	thrombus	1414:1421	arg1	components					1423:1432	most thrombus components	1409:1432	most thrombus components	1409:1432	Our detailed structural analysis also showed that most thrombus components are localized in the white thrombus regardless of endotoxemia.					
36696220	6	26	theme	approach	1100:1107	arg1	feasibility					1080:1090	the feasibility	1076:1090	the feasibility of this approach in analyzing the structural composition of thrombi	1076:1158	We also performed a proof-of-concept study of imaging mass cytometry to assess the feasibility of this approach in analyzing the structural composition of thrombi.					
36696220	0	27	from	role	31:34	arg1	thrombosis					95:104	endotoxemia-enhanced deep vein thrombosis	64:104	endotoxemia-enhanced deep vein thrombosis in mice	64:112	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.					
36696220	9	28	with	interactions	1570:1581	arg1	constituents					1610:1621	other thrombus constituents	1595:1621	other thrombus constituents	1595:1621	Moreover, although endotoxemia modulated the relative representation and interactions of VWF with other thrombus constituents, the scaffolding network, comprised VWF, fibrin, and neutrophil extracellular traps, remained largely unaffected.					
36696220	4	29	theme	cytometry	732:740	arg1	study					710:714	a proof-of-concept study	691:714	a proof-of-concept study of imaging mass cytometry to quantitatively and simultaneously analyze the localizations and interactions of 10 venous thrombus constituents	691:855	We also performed a proof-of-concept study of imaging mass cytometry to quantitatively and simultaneously analyze the localizations and interactions of 10 venous thrombus constituents.					
36696220	7	30	theme	higher	1233:1238	arg1	incidences					1240:1249	significantly higher incidences	1219:1249	significantly higher incidences of venous thrombosis	1219:1270	RESULTS We found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies.					
36696220	7	30	theme	higher	1233:1238	arg1	effect					1276:1281	an effect	1273:1281	an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies	1273:1356	RESULTS We found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies.					
36696220	1	31	theme	deep	275:278	arg1	thrombosis					285:294	deep vein thrombosis	275:294	deep vein thrombosis	275:294	BACKGROUND Although the concept of immunothrombosis has established a link between inflammation and thrombosis, the role of inflammation in the pathogenesis of deep vein thrombosis remains to be fully elucidated.					
36696220	5	32	theme	lipopolysaccharide	956:973	arg1	model					975:979	the lipopolysaccharide model	952:979	the lipopolysaccharide model of endotoxemia	952:994	METHODS We combined the murine inferior vena cava stenosis model of deep vein thrombosis with the lipopolysaccharide model of endotoxemia.					
36696220	9	33	with	representation	1551:1564	arg1	constituents					1610:1621	other thrombus constituents	1595:1621	other thrombus constituents	1595:1621	Moreover, although endotoxemia modulated the relative representation and interactions of VWF with other thrombus constituents, the scaffolding network, comprised VWF, fibrin, and neutrophil extracellular traps, remained largely unaffected.					
36696220	4	34	theme	venous	828:833	arg1	constituents					844:855	10 venous thrombus constituents	825:855	10 venous thrombus constituents	825:855	We also performed a proof-of-concept study of imaging mass cytometry to quantitatively and simultaneously analyze the localizations and interactions of 10 venous thrombus constituents.					
36696220	1	35	theme	thrombosis	285:294	arg1	pathogenesis					259:270	the pathogenesis	255:270	the pathogenesis of deep vein thrombosis	255:294	BACKGROUND Although the concept of immunothrombosis has established a link between inflammation and thrombosis, the role of inflammation in the pathogenesis of deep vein thrombosis remains to be fully elucidated.					
36696220	8	36	theme	white	1455:1459	arg1	thrombus					1461:1468	the white thrombus	1451:1468	the white thrombus regardless of endotoxemia	1451:1494	Our detailed structural analysis also showed that most thrombus components are localized in the white thrombus regardless of endotoxemia.					
36696220	7	37	theme	lipopolysaccharide-treated	1183:1208	arg1	mice					1210:1213	lipopolysaccharide-treated mice	1183:1213	lipopolysaccharide-treated mice	1183:1213	RESULTS We found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies.					
36696220	10	38	theme	venous	1957:1962	arg1	thrombi					1964:1970	venous thrombi	1957:1970	venous thrombi	1957:1970	CONCLUSIONS We observe a key role for VWF in the pathogenesis of inflammation-associated venous thrombosis while providing a more comprehensive insight into the molecular interactions that constitute the architecture of venous thrombi.					
36696220	4	39	theme	constituents	844:855	arg1	interactions					809:820	interactions	809:820	interactions	809:820	We also performed a proof-of-concept study of imaging mass cytometry to quantitatively and simultaneously analyze the localizations and interactions of 10 venous thrombus constituents.					
36696220	4	39	theme	constituents	844:855	arg1	localizations					791:803	localizations	791:803	localizations	791:803	We also performed a proof-of-concept study of imaging mass cytometry to quantitatively and simultaneously analyze the localizations and interactions of 10 venous thrombus constituents.					
36696220	0	40	theme	von	39:41	arg1	factor					54:59	von Willebrand factor	39:59	von Willebrand factor	39:59	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.					
36696220	9	41	theme	extracellular	1687:1699	arg1	neutrophil					1676:1685	neutrophil extracellular	1676:1699	neutrophil extracellular	1676:1699	Moreover, although endotoxemia modulated the relative representation and interactions of VWF with other thrombus constituents, the scaffolding network, comprised VWF, fibrin, and neutrophil extracellular traps, remained largely unaffected.					
36696220	7	42	theme	inhibitory	1331:1340	arg1	antibodies					1347:1356	inhibitory αVWF antibodies	1331:1356	inhibitory αVWF antibodies	1331:1356	RESULTS We found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies.					
36696220	5	43	theme	stenosis	908:915	arg1	model					917:921	the murine inferior vena cava stenosis model	878:921	the murine inferior vena cava stenosis model of deep vein thrombosis	878:945	METHODS We combined the murine inferior vena cava stenosis model of deep vein thrombosis with the lipopolysaccharide model of endotoxemia.					
36696220	3	44	theme	venous	654:659	arg1	thrombosis					661:670	inflammation-associated venous thrombosis	630:670	inflammation-associated venous thrombosis	630:670	OBJECTIVES The objective of this study was to investigate the role of the von Willebrand factor (VWF) in inflammation-associated venous thrombosis.					
36696220	10	45	theme	venous	1826:1831	arg1	thrombosis					1833:1842	inflammation-associated venous thrombosis	1802:1842	inflammation-associated venous thrombosis	1802:1842	CONCLUSIONS We observe a key role for VWF in the pathogenesis of inflammation-associated venous thrombosis while providing a more comprehensive insight into the molecular interactions that constitute the architecture of venous thrombi.					
36696220	10	46	from	role	1766:1769	arg1	pathogenesis					1786:1797	the pathogenesis	1782:1797	the pathogenesis of inflammation-associated venous thrombosis	1782:1842	CONCLUSIONS We observe a key role for VWF in the pathogenesis of inflammation-associated venous thrombosis while providing a more comprehensive insight into the molecular interactions that constitute the architecture of venous thrombi.					
36696220	6	47	theme	structural	1126:1135	arg1	composition					1137:1147	the structural composition	1122:1147	the structural composition of thrombi	1122:1158	We also performed a proof-of-concept study of imaging mass cytometry to assess the feasibility of this approach in analyzing the structural composition of thrombi.					
36696220	5	48	theme	inferior	889:896	arg1	model					917:921	the murine inferior vena cava stenosis model	878:921	the murine inferior vena cava stenosis model of deep vein thrombosis	878:945	METHODS We combined the murine inferior vena cava stenosis model of deep vein thrombosis with the lipopolysaccharide model of endotoxemia.					
36696220	10	49	theme	comprehensive	1867:1879	arg1	insight					1881:1887	a more comprehensive insight	1860:1887	a more comprehensive insight into the molecular interactions that constitute the architecture of venous thrombi	1860:1970	CONCLUSIONS We observe a key role for VWF in the pathogenesis of inflammation-associated venous thrombosis while providing a more comprehensive insight into the molecular interactions that constitute the architecture of venous thrombi.					
36696220	2	50	theme	venous	370:375	arg1	thrombi					377:383	venous thrombi	370:383	venous thrombi	370:383	Further, although various constituents of venous thrombi have been identified, their localizations and cellular and molecular interactions are yet to be combined in a single, multiplexed analysis.					
36696220	5	51	theme	vena	898:901	arg1	model					917:921	the murine inferior vena cava stenosis model	878:921	the murine inferior vena cava stenosis model of deep vein thrombosis	878:945	METHODS We combined the murine inferior vena cava stenosis model of deep vein thrombosis with the lipopolysaccharide model of endotoxemia.					
36696220	10	52	theme	molecular	1898:1906	arg1	interactions					1908:1919	the molecular interactions	1894:1919	the molecular interactions that constitute the architecture of venous thrombi	1894:1970	CONCLUSIONS We observe a key role for VWF in the pathogenesis of inflammation-associated venous thrombosis while providing a more comprehensive insight into the molecular interactions that constitute the architecture of venous thrombi.					
36696220	5	53	theme	deep	926:929	arg1	thrombosis					936:945	deep vein thrombosis	926:945	deep vein thrombosis	926:945	METHODS We combined the murine inferior vena cava stenosis model of deep vein thrombosis with the lipopolysaccharide model of endotoxemia.					
36696220	4	54	theme	proof-of-concept	693:708	arg1	study					710:714	a proof-of-concept study	691:714	a proof-of-concept study of imaging mass cytometry to quantitatively and simultaneously analyze the localizations and interactions of 10 venous thrombus constituents	691:855	We also performed a proof-of-concept study of imaging mass cytometry to quantitatively and simultaneously analyze the localizations and interactions of 10 venous thrombus constituents.					
36696220	3	55	theme	Willebrand	603:612	arg1	VWF					622:624	VWF	622:624	VWF	622:624	OBJECTIVES The objective of this study was to investigate the role of the von Willebrand factor (VWF) in inflammation-associated venous thrombosis.					
36696220	3	55	theme	Willebrand	603:612	arg1	factor					614:619	von Willebrand factor	599:619	the von Willebrand factor (VWF)	595:625	OBJECTIVES The objective of this study was to investigate the role of the von Willebrand factor (VWF) in inflammation-associated venous thrombosis.					
36696220	9	56	theme	VWF	1586:1588	arg1	interactions					1570:1581	interactions	1570:1581	interactions of VWF with other thrombus constituents	1570:1621	Moreover, although endotoxemia modulated the relative representation and interactions of VWF with other thrombus constituents, the scaffolding network, comprised VWF, fibrin, and neutrophil extracellular traps, remained largely unaffected.					
36696220	9	56	theme	VWF	1586:1588	arg1	representation					1551:1564	the relative representation	1538:1564	the relative representation	1538:1564	Moreover, although endotoxemia modulated the relative representation and interactions of VWF with other thrombus constituents, the scaffolding network, comprised VWF, fibrin, and neutrophil extracellular traps, remained largely unaffected.					
36696220	6	57	theme	cytometry	1056:1064	arg1	study					1034:1038	a proof-of-concept study	1015:1038	a proof-of-concept study of imaging mass cytometry	1015:1064	We also performed a proof-of-concept study of imaging mass cytometry to assess the feasibility of this approach in analyzing the structural composition of thrombi.					
36696220	0	58	from	thrombosis	95:104	arg1	mice					109:112	mice	109:112	mice	109:112	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.					
36696220	9	59	theme	other	1595:1599	arg1	constituents					1610:1621	other thrombus constituents	1595:1621	other thrombus constituents	1595:1621	Moreover, although endotoxemia modulated the relative representation and interactions of VWF with other thrombus constituents, the scaffolding network, comprised VWF, fibrin, and neutrophil extracellular traps, remained largely unaffected.					
36696220	6	60	theme	imaging	1043:1049	arg1	cytometry					1056:1064	imaging mass cytometry	1043:1064	imaging mass cytometry	1043:1064	We also performed a proof-of-concept study of imaging mass cytometry to assess the feasibility of this approach in analyzing the structural composition of thrombi.					
36696220	8	61	theme	detailed	1363:1370	arg1	analysis					1383:1390	Our detailed structural analysis	1359:1390	Our detailed structural analysis	1359:1390	Our detailed structural analysis also showed that most thrombus components are localized in the white thrombus regardless of endotoxemia.					
36696220	7	62	theme	thrombosis	1261:1270	arg1	incidences					1240:1249	significantly higher incidences	1219:1249	significantly higher incidences of venous thrombosis	1219:1270	RESULTS We found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies.					
36696220	7	62	theme	thrombosis	1261:1270	arg1	effect					1276:1281	an effect	1273:1281	an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies	1273:1356	RESULTS We found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies.					
36696220	0	63	theme	deep	85:88	arg1	thrombosis					95:104	endotoxemia-enhanced deep vein thrombosis	64:104	endotoxemia-enhanced deep vein thrombosis in mice	64:112	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.					
36696220	1	64	theme	immunothrombosis	150:165	arg1	concept					139:145	the concept	135:145	the concept of immunothrombosis	135:165	BACKGROUND Although the concept of immunothrombosis has established a link between inflammation and thrombosis, the role of inflammation in the pathogenesis of deep vein thrombosis remains to be fully elucidated.					
36696220	7	65	theme	venous	1254:1259	arg1	thrombosis					1261:1270	venous thrombosis	1254:1270	venous thrombosis	1254:1270	RESULTS We found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies.					
36696220	5	66	theme	endotoxemia	984:994	arg1	model					975:979	the lipopolysaccharide model	952:979	the lipopolysaccharide model of endotoxemia	952:994	METHODS We combined the murine inferior vena cava stenosis model of deep vein thrombosis with the lipopolysaccharide model of endotoxemia.					
36696220	5	67	theme	thrombosis	936:945	arg1	model					917:921	the murine inferior vena cava stenosis model	878:921	the murine inferior vena cava stenosis model of deep vein thrombosis	878:945	METHODS We combined the murine inferior vena cava stenosis model of deep vein thrombosis with the lipopolysaccharide model of endotoxemia.					
36696220	8	68	theme	most	1409:1412	arg1	components					1423:1432	most thrombus components	1409:1432	most thrombus components	1409:1432	Our detailed structural analysis also showed that most thrombus components are localized in the white thrombus regardless of endotoxemia.					
36696220	0	69	theme	mechanistic	4:14	arg1	role					31:34	The mechanistic and structural role	0:34	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.	0:113	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.					
36696220	2	70	theme	molecular	444:452	arg1	interactions					454:465	cellular and molecular interactions	431:465	cellular and molecular interactions	431:465	Further, although various constituents of venous thrombi have been identified, their localizations and cellular and molecular interactions are yet to be combined in a single, multiplexed analysis.					
36696220	4	71	theme	mass	727:730	arg1	cytometry					732:740	imaging mass cytometry	719:740	imaging mass cytometry	719:740	We also performed a proof-of-concept study of imaging mass cytometry to quantitatively and simultaneously analyze the localizations and interactions of 10 venous thrombus constituents.					
36696220	2	72	theme	thrombi	377:383	arg1	constituents					354:365	various constituents	346:365	various constituents of venous thrombi	346:383	Further, although various constituents of venous thrombi have been identified, their localizations and cellular and molecular interactions are yet to be combined in a single, multiplexed analysis.					
36696220	0	73	theme	structural	20:29	arg1	role					31:34	The mechanistic and structural role	0:34	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.	0:113	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.					
36696220	1	74	theme	vein	280:283	arg1	thrombosis					285:294	deep vein thrombosis	275:294	deep vein thrombosis	275:294	BACKGROUND Although the concept of immunothrombosis has established a link between inflammation and thrombosis, the role of inflammation in the pathogenesis of deep vein thrombosis remains to be fully elucidated.					
36696220	2	75	theme	cellular	431:438	arg1	interactions					454:465	cellular and molecular interactions	431:465	cellular and molecular interactions	431:465	Further, although various constituents of venous thrombi have been identified, their localizations and cellular and molecular interactions are yet to be combined in a single, multiplexed analysis.					
36696220	3	76	from	role	587:590	arg1	thrombosis					661:670	inflammation-associated venous thrombosis	630:670	inflammation-associated venous thrombosis	630:670	OBJECTIVES The objective of this study was to investigate the role of the von Willebrand factor (VWF) in inflammation-associated venous thrombosis.					
36696220	10	77	theme	key	1762:1764	arg1	role					1766:1769	a key role	1760:1769	a key role for VWF in the pathogenesis of inflammation-associated venous thrombosis	1760:1842	CONCLUSIONS We observe a key role for VWF in the pathogenesis of inflammation-associated venous thrombosis while providing a more comprehensive insight into the molecular interactions that constitute the architecture of venous thrombi.					
36696220	7	78	theme	αVWF	1342:1345	arg1	antibodies					1347:1356	inhibitory αVWF antibodies	1331:1356	inhibitory αVWF antibodies	1331:1356	RESULTS We found that lipopolysaccharide-treated mice had significantly higher incidences of venous thrombosis, an effect that was mitigated when VWF was inhibited using inhibitory αVWF antibodies.					
36696220	10	79	theme	thrombi	1964:1970	arg1	architecture					1941:1952	the architecture	1937:1952	the architecture of venous thrombi	1937:1970	CONCLUSIONS We observe a key role for VWF in the pathogenesis of inflammation-associated venous thrombosis while providing a more comprehensive insight into the molecular interactions that constitute the architecture of venous thrombi.					
36696220	5	80	dep	vena	898:901	arg1	cava					903:906	cava	903:906	cava	903:906	METHODS We combined the murine inferior vena cava stenosis model of deep vein thrombosis with the lipopolysaccharide model of endotoxemia.					
36696220	0	81	theme	Willebrand	43:52	arg1	factor					54:59	von Willebrand factor	39:59	von Willebrand factor	39:59	The mechanistic and structural role of von Willebrand factor in endotoxemia-enhanced deep vein thrombosis in mice.					
36696220	4	82	theme	thrombus	835:842	arg1	constituents					844:855	10 venous thrombus constituents	825:855	10 venous thrombus constituents	825:855	We also performed a proof-of-concept study of imaging mass cytometry to quantitatively and simultaneously analyze the localizations and interactions of 10 venous thrombus constituents.					
36696220	10	83	dep	CONCLUSIONS	1737:1747	arg1	observe					1752:1758	observe	1752:1758	observe a key role for VWF in the pathogenesis of inflammation-associated venous thrombosis while providing a more comprehensive insight into the molecular interactions that constitute the architecture of venous thrombi	1752:1970	CONCLUSIONS We observe a key role for VWF in the pathogenesis of inflammation-associated venous thrombosis while providing a more comprehensive insight into the molecular interactions that constitute the architecture of venous thrombi.					
36696220	6	84	theme	thrombi	1152:1158	arg1	composition					1137:1147	the structural composition	1122:1147	the structural composition of thrombi	1122:1158	We also performed a proof-of-concept study of imaging mass cytometry to assess the feasibility of this approach in analyzing the structural composition of thrombi.					
36696220	3	85	theme	inflammation-associated	630:652	arg1	thrombosis					661:670	inflammation-associated venous thrombosis	630:670	inflammation-associated venous thrombosis	630:670	OBJECTIVES The objective of this study was to investigate the role of the von Willebrand factor (VWF) in inflammation-associated venous thrombosis.					
36696220	5	86	dep	METHODS	858:864	arg1	combined					869:876	combined	869:876	combined the murine inferior vena cava stenosis model of deep vein thrombosis with the lipopolysaccharide model of endotoxemia	869:994	METHODS We combined the murine inferior vena cava stenosis model of deep vein thrombosis with the lipopolysaccharide model of endotoxemia.					
36696220	10	87	theme	inflammation-associated	1802:1824	arg1	thrombosis					1833:1842	inflammation-associated venous thrombosis	1802:1842	inflammation-associated venous thrombosis	1802:1842	CONCLUSIONS We observe a key role for VWF in the pathogenesis of inflammation-associated venous thrombosis while providing a more comprehensive insight into the molecular interactions that constitute the architecture of venous thrombi.					
36696220	1	88	theme	inflammation	239:250	arg1	role					231:234	the role	227:234	the role of inflammation in the pathogenesis of deep vein thrombosis	227:294	BACKGROUND Although the concept of immunothrombosis has established a link between inflammation and thrombosis, the role of inflammation in the pathogenesis of deep vein thrombosis remains to be fully elucidated.					
36677963	2	0	theme	resonance	466:474	arg1	peak					482:485	a UV-Vis surface plasmon resonance (SPR) peak	441:485	a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm	441:496	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	3	1	dep	reduction	615:623	arg1	the					611:613	the	611:613	the	611:613	The FTIR spectra showed that the abundance of functional groups present in the carrageenan were responsible for the reduction and stabilisation of the c-AgNPs.					
36677963	2	2	theme	plasmon	458:464	arg1	SPR					477:479	SPR	477:479	SPR	477:479	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	2	2	theme	plasmon	458:464	arg1	resonance					466:474	a UV-Vis surface plasmon resonance	441:474	a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm	441:496	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	4	3	theme	composition	810:820	arg1	presence					791:798	the presence	787:798	the presence of a high composition of elemental silver (85.87 wt%)	787:852	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	1	4	theme	flower-like	174:184	arg1	c-AgNPs					220:226	c-AgNPs	220:226	c-AgNPs	220:226	Herein, we report the green synthesis of flower-like carrageenan-silver nanoparticles (c-AgNPs) through a facile hydrothermal reaction at 90 °C for 2 h.					
36677963	1	4	theme	flower-like	174:184	arg1	nanoparticles					205:217	flower-like carrageenan-silver nanoparticles	174:217	flower-like carrageenan-silver nanoparticles (c-AgNPs)	174:227	Herein, we report the green synthesis of flower-like carrageenan-silver nanoparticles (c-AgNPs) through a facile hydrothermal reaction at 90 °C for 2 h.					
36677963	0	5	theme	Properties	121:130	arg1	Synthesis					6:14	Green Synthesis	0:14	Green Synthesis of Flower-Like Carrageenan-Silver Nanoparticles	0:62	Green Synthesis of Flower-Like Carrageenan-Silver Nanoparticles and Elucidation of Its Physicochemical and Antibacterial Properties.					
36677963	0	5	theme	Properties	121:130	arg1	Elucidation					68:78	Elucidation	68:78	Elucidation of Its Physicochemical and Antibacterial Properties	68:130	Green Synthesis of Flower-Like Carrageenan-Silver Nanoparticles and Elucidation of Its Physicochemical and Antibacterial Properties.					
36677963	3	6	theme	c-AgNPs	650:656	arg1	stabilisation					629:641	stabilisation	629:641	stabilisation	629:641	The FTIR spectra showed that the abundance of functional groups present in the carrageenan were responsible for the reduction and stabilisation of the c-AgNPs.					
36677963	3	6	theme	c-AgNPs	650:656	arg1	reduction					615:623	reduction	615:623	reduction	615:623	The FTIR spectra showed that the abundance of functional groups present in the carrageenan were responsible for the reduction and stabilisation of the c-AgNPs.					
36677963	4	7	theme	silver	835:840	arg1	composition					810:820	a high composition	803:820	a high composition of elemental silver (85.87 wt%)	803:852	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	6	8	theme	c-AgNPs	1095:1101	arg1	mechanism					1070:1078	The growth mechanism	1059:1078	The growth mechanism of flower-like c-AgNPs	1059:1101	The growth mechanism of flower-like c-AgNPs were elucidated based on the TEM and AFM analyses.					
36677963	0	9	theme	Antibacterial	107:119	arg1	Properties					121:130	Its Physicochemical and Antibacterial Properties	83:130	Its Physicochemical and Antibacterial Properties	83:130	Green Synthesis of Flower-Like Carrageenan-Silver Nanoparticles and Elucidation of Its Physicochemical and Antibacterial Properties.					
36677963	4	10	theme	cubic	725:729	arg1	structure					731:739	face-centred cubic structure	712:739	face-centred cubic structure	712:739	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	3	11	theme	FTIR	503:506	arg1	spectra					508:514	The FTIR spectra	499:514	The FTIR spectra	499:514	The FTIR spectra showed that the abundance of functional groups present in the carrageenan were responsible for the reduction and stabilisation of the c-AgNPs.					
36677963	3	12	from	present	563:569	arg1	carrageenan					578:588	the carrageenan	574:588	the carrageenan	574:588	The FTIR spectra showed that the abundance of functional groups present in the carrageenan were responsible for the reduction and stabilisation of the c-AgNPs.					
36677963	7	13	theme	inhibition	1256:1265	arg1	zones					1247:1251	zones	1247:1251	zones of inhibition ranging from 8.0 ± 0.0 to 11.7 ± 0.6 mm and 7.3 ± 0.6 to 9.7 ± 0.6 mm	1247:1335	The c-AgNPs displayed promising antibacterial properties against E. coli and S. aureus, with zones of inhibition ranging from 8.0 ± 0.0 to 11.7 ± 0.6 mm and 7.3 ± 0.6 to 9.7 ± 0.6 mm, respectively, as the concentration of c-AgNPs increased from 0.1 to 4 mg/mL.					
36677963	7	14	theme	±	1328:1328	arg1	mm					1334:1335	7.3 ± 0.6 to 9.7 ± 0.6 mm	1311:1335	7.3 ± 0.6 to 9.7 ± 0.6 mm	1311:1335	The c-AgNPs displayed promising antibacterial properties against E. coli and S. aureus, with zones of inhibition ranging from 8.0 ± 0.0 to 11.7 ± 0.6 mm and 7.3 ± 0.6 to 9.7 ± 0.6 mm, respectively, as the concentration of c-AgNPs increased from 0.1 to 4 mg/mL.					
36677963	1	15	theme	carrageenan-silver	186:203	arg1	c-AgNPs					220:226	c-AgNPs	220:226	c-AgNPs	220:226	Herein, we report the green synthesis of flower-like carrageenan-silver nanoparticles (c-AgNPs) through a facile hydrothermal reaction at 90 °C for 2 h.					
36677963	1	15	theme	carrageenan-silver	186:203	arg1	nanoparticles					205:217	flower-like carrageenan-silver nanoparticles	174:217	flower-like carrageenan-silver nanoparticles (c-AgNPs)	174:227	Herein, we report the green synthesis of flower-like carrageenan-silver nanoparticles (c-AgNPs) through a facile hydrothermal reaction at 90 °C for 2 h.					
36677963	0	16	theme	Nanoparticles	50:62	arg1	Synthesis					6:14	Green Synthesis	0:14	Green Synthesis of Flower-Like Carrageenan-Silver Nanoparticles	0:62	Green Synthesis of Flower-Like Carrageenan-Silver Nanoparticles and Elucidation of Its Physicochemical and Antibacterial Properties.					
36677963	0	16	theme	Nanoparticles	50:62	arg1	Elucidation					68:78	Elucidation	68:78	Elucidation of Its Physicochemical and Antibacterial Properties	68:130	Green Synthesis of Flower-Like Carrageenan-Silver Nanoparticles and Elucidation of Its Physicochemical and Antibacterial Properties.					
36677963	7	17	theme	±	1298:1298	arg1	mm					1304:1305	8.0 ± 0.0 to 11.7 ± 0.6 mm	1280:1305	8.0 ± 0.0 to 11.7 ± 0.6 mm	1280:1305	The c-AgNPs displayed promising antibacterial properties against E. coli and S. aureus, with zones of inhibition ranging from 8.0 ± 0.0 to 11.7 ± 0.6 mm and 7.3 ± 0.6 to 9.7 ± 0.6 mm, respectively, as the concentration of c-AgNPs increased from 0.1 to 4 mg/mL.					
36677963	1	18	theme	nanoparticles	205:217	arg1	synthesis					161:169	the green synthesis	151:169	the green synthesis of flower-like carrageenan-silver nanoparticles (c-AgNPs)	151:227	Herein, we report the green synthesis of flower-like carrageenan-silver nanoparticles (c-AgNPs) through a facile hydrothermal reaction at 90 °C for 2 h.					
36677963	0	19	theme	Physicochemical	87:101	arg1	Properties					121:130	Its Physicochemical and Antibacterial Properties	83:130	Its Physicochemical and Antibacterial Properties	83:130	Green Synthesis of Flower-Like Carrageenan-Silver Nanoparticles and Elucidation of Its Physicochemical and Antibacterial Properties.					
36677963	4	20	theme	high	805:808	arg1	composition					810:820	a high composition	803:820	a high composition of elemental silver (85.87 wt%)	803:852	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	3	21	from	carrageenan	578:588	arg1	present					563:569	present	563:569	present	563:569	The FTIR spectra showed that the abundance of functional groups present in the carrageenan were responsible for the reduction and stabilisation of the c-AgNPs.					
36677963	0	22	theme	Green	0:4	arg1	Synthesis					6:14	Green Synthesis	0:14	Green Synthesis of Flower-Like Carrageenan-Silver Nanoparticles	0:62	Green Synthesis of Flower-Like Carrageenan-Silver Nanoparticles and Elucidation of Its Physicochemical and Antibacterial Properties.					
36677963	4	23	theme	crystalline	689:699	arg1	nature					701:706	crystalline nature	689:706	crystalline nature	689:706	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	0	24	theme	Flower-Like	19:29	arg1	Nanoparticles					50:62	Flower-Like Carrageenan-Silver Nanoparticles	19:62	Flower-Like Carrageenan-Silver Nanoparticles	19:62	Green Synthesis of Flower-Like Carrageenan-Silver Nanoparticles and Elucidation of Its Physicochemical and Antibacterial Properties.					
36677963	2	25	theme	nitrate	310:316	arg1	reduction					290:298	The reduction	286:298	The reduction of silver nitrate (AgNO3) to c-AgNPs	286:335	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	2	25	theme	nitrate	310:316	arg1	evident					341:347	evident	341:347	evident	341:347	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	5	26	theme	c-AgNPs	962:968	arg1	formation					937:945	the formation	933:945	the formation of flower-like c-AgNPs composed of intercrossed and random lamellar petals of approximately 50 nm in thickness	933:1056	Interestingly, the morphological characterisations by SEM and FE-SEM revealed the formation of flower-like c-AgNPs composed of intercrossed and random lamellar petals of approximately 50 nm in thickness.					
36677963	2	27	theme	dark	405:408	arg1	brown					410:414	dark brown	405:414	dark brown	405:414	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	5	28	theme	morphological	874:886	arg1	characterisations					888:904	the morphological characterisations	870:904	the morphological characterisations by SEM and FE-SEM	870:922	Interestingly, the morphological characterisations by SEM and FE-SEM revealed the formation of flower-like c-AgNPs composed of intercrossed and random lamellar petals of approximately 50 nm in thickness.					
36677963	2	29	theme	silver	303:308	arg1	nitrate					310:316	silver nitrate	303:316	silver nitrate (AgNO3)	303:324	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	2	29	theme	silver	303:308	arg1	AgNO3					319:323	AgNO3	319:323	AgNO3	319:323	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	1	30	theme	green	155:159	arg1	synthesis					161:169	the green synthesis	151:169	the green synthesis of flower-like carrageenan-silver nanoparticles (c-AgNPs)	151:227	Herein, we report the green synthesis of flower-like carrageenan-silver nanoparticles (c-AgNPs) through a facile hydrothermal reaction at 90 °C for 2 h.					
36677963	2	31	theme	surface	450:456	arg1	SPR					477:479	SPR	477:479	SPR	477:479	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	2	31	theme	surface	450:456	arg1	resonance					466:474	a UV-Vis surface plasmon resonance	441:474	a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm	441:496	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	1	32	from	°C	274:275	arg1	reaction					259:266	a facile hydrothermal reaction	237:266	a facile hydrothermal reaction at 90 °C for 2 h	237:283	Herein, we report the green synthesis of flower-like carrageenan-silver nanoparticles (c-AgNPs) through a facile hydrothermal reaction at 90 °C for 2 h.					
36677963	3	33	theme	functional	545:554	arg1	groups					556:561	functional groups	545:561	functional groups present in the carrageenan	545:588	The FTIR spectra showed that the abundance of functional groups present in the carrageenan were responsible for the reduction and stabilisation of the c-AgNPs.					
36677963	7	34	theme	±	1315:1315	arg1	mm					1334:1335	7.3 ± 0.6 to 9.7 ± 0.6 mm	1311:1335	7.3 ± 0.6 to 9.7 ± 0.6 mm	1311:1335	The c-AgNPs displayed promising antibacterial properties against E. coli and S. aureus, with zones of inhibition ranging from 8.0 ± 0.0 to 11.7 ± 0.6 mm and 7.3 ± 0.6 to 9.7 ± 0.6 mm, respectively, as the concentration of c-AgNPs increased from 0.1 to 4 mg/mL.					
36677963	7	35	dep	11.7	1293:1296	arg1	to					1290:1291	to	1290:1291	to	1290:1291	The c-AgNPs displayed promising antibacterial properties against E. coli and S. aureus, with zones of inhibition ranging from 8.0 ± 0.0 to 11.7 ± 0.6 mm and 7.3 ± 0.6 to 9.7 ± 0.6 mm, respectively, as the concentration of c-AgNPs increased from 0.1 to 4 mg/mL.					
36677963	0	36	theme	Carrageenan-Silver	31:48	arg1	Nanoparticles					50:62	Flower-Like Carrageenan-Silver Nanoparticles	19:62	Flower-Like Carrageenan-Silver Nanoparticles	19:62	Green Synthesis of Flower-Like Carrageenan-Silver Nanoparticles and Elucidation of Its Physicochemical and Antibacterial Properties.					
36677963	2	37	theme	UV-Vis	443:448	arg1	SPR					477:479	SPR	477:479	SPR	477:479	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	2	37	theme	UV-Vis	443:448	arg1	resonance					466:474	a UV-Vis surface plasmon resonance	441:474	a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm	441:496	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	6	38	theme	TEM	1132:1134	arg1	analyses					1144:1151	the TEM and AFM analyses	1128:1151	the TEM and AFM analyses	1128:1151	The growth mechanism of flower-like c-AgNPs were elucidated based on the TEM and AFM analyses.					
36677963	3	39	theme	groups	556:561	arg1	abundance					532:540	the abundance	528:540	the abundance of functional groups present in the carrageenan	528:588	The FTIR spectra showed that the abundance of functional groups present in the carrageenan were responsible for the reduction and stabilisation of the c-AgNPs.					
36677963	3	39	theme	groups	556:561	arg1	responsible					595:605	responsible	595:605	responsible	595:605	The FTIR spectra showed that the abundance of functional groups present in the carrageenan were responsible for the reduction and stabilisation of the c-AgNPs.					
36677963	1	40	theme	facile	239:244	arg1	reaction					259:266	a facile hydrothermal reaction	237:266	a facile hydrothermal reaction at 90 °C for 2 h	237:283	Herein, we report the green synthesis of flower-like carrageenan-silver nanoparticles (c-AgNPs) through a facile hydrothermal reaction at 90 °C for 2 h.					
36677963	6	41	theme	flower-like	1083:1093	arg1	c-AgNPs					1095:1101	flower-like c-AgNPs	1083:1101	flower-like c-AgNPs	1083:1101	The growth mechanism of flower-like c-AgNPs were elucidated based on the TEM and AFM analyses.					
36677963	2	42	theme	solution	377:384	arg1	change					363:368	the colour change	352:368	the colour change of the solution	352:384	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	2	43	dep	brown	410:414	arg1	colourless					391:400	colourless	391:400	colourless	391:400	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	7	44	theme	±	1284:1284	arg1	mm					1304:1305	8.0 ± 0.0 to 11.7 ± 0.6 mm	1280:1305	8.0 ± 0.0 to 11.7 ± 0.6 mm	1280:1305	The c-AgNPs displayed promising antibacterial properties against E. coli and S. aureus, with zones of inhibition ranging from 8.0 ± 0.0 to 11.7 ± 0.6 mm and 7.3 ± 0.6 to 9.7 ± 0.6 mm, respectively, as the concentration of c-AgNPs increased from 0.1 to 4 mg/mL.					
36677963	4	45	theme	XRD	663:665	arg1	pattern					667:673	The XRD pattern	659:673	The XRD pattern	659:673	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	6	46	theme	AFM	1140:1142	arg1	analyses					1144:1151	the TEM and AFM analyses	1128:1151	the TEM and AFM analyses	1128:1151	The growth mechanism of flower-like c-AgNPs were elucidated based on the TEM and AFM analyses.					
36677963	5	47	theme	random	999:1004	arg1	petals					1015:1020	random lamellar petals	999:1020	random lamellar petals	999:1020	Interestingly, the morphological characterisations by SEM and FE-SEM revealed the formation of flower-like c-AgNPs composed of intercrossed and random lamellar petals of approximately 50 nm in thickness.					
36677963	7	48	dep	9.7	1324:1326	arg1	to					1321:1322	to	1321:1322	to	1321:1322	The c-AgNPs displayed promising antibacterial properties against E. coli and S. aureus, with zones of inhibition ranging from 8.0 ± 0.0 to 11.7 ± 0.6 mm and 7.3 ± 0.6 to 9.7 ± 0.6 mm, respectively, as the concentration of c-AgNPs increased from 0.1 to 4 mg/mL.					
36677963	4	49	theme	EDX	767:769	arg1	analysis					771:778	the EDX analysis	763:778	the EDX analysis	763:778	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	4	50	dep	nature	701:706	arg1	the					685:687	the	685:687	the	685:687	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	4	51	theme	face-centred	712:723	arg1	structure					731:739	face-centred cubic structure	712:739	face-centred cubic structure	712:739	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	1	52	theme	hydrothermal	246:257	arg1	reaction					259:266	a facile hydrothermal reaction	237:266	a facile hydrothermal reaction at 90 °C for 2 h	237:283	Herein, we report the green synthesis of flower-like carrageenan-silver nanoparticles (c-AgNPs) through a facile hydrothermal reaction at 90 °C for 2 h.					
36677963	4	53	theme	wt	849:850	arg1	%					851:851	85.87 wt%	843:851	85.87 wt%	843:851	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	4	53	theme	wt	849:850	arg1	silver					835:840	elemental silver	825:840	elemental silver (85.87 wt%)	825:852	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	5	54	theme	lamellar	1006:1013	arg1	petals					1015:1020	random lamellar petals	999:1020	random lamellar petals	999:1020	Interestingly, the morphological characterisations by SEM and FE-SEM revealed the formation of flower-like c-AgNPs composed of intercrossed and random lamellar petals of approximately 50 nm in thickness.					
36677963	4	55	theme	c-AgNPs	748:754	arg1	structure					731:739	face-centred cubic structure	712:739	face-centred cubic structure	712:739	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	4	55	theme	c-AgNPs	748:754	arg1	nature					701:706	crystalline nature	689:706	crystalline nature	689:706	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	6	56	theme	growth	1063:1068	arg1	mechanism					1070:1078	The growth mechanism	1059:1078	The growth mechanism of flower-like c-AgNPs	1059:1101	The growth mechanism of flower-like c-AgNPs were elucidated based on the TEM and AFM analyses.					
36677963	4	57	theme	elemental	825:833	arg1	%					851:851	85.87 wt%	843:851	85.87 wt%	843:851	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	4	57	theme	elemental	825:833	arg1	silver					835:840	elemental silver	825:840	elemental silver (85.87 wt%)	825:852	The XRD pattern confirmed the crystalline nature and face-centred cubic structure of the c-AgNPs, while the EDX analysis showed the presence of a high composition of elemental silver (85.87 wt%).					
36677963	2	58	from	nm	495:496	arg1	peak					482:485	a UV-Vis surface plasmon resonance (SPR) peak	441:485	a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm	441:496	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	7	59	theme	promising	1176:1184	arg1	properties					1200:1209	promising antibacterial properties	1176:1209	promising antibacterial properties	1176:1209	The c-AgNPs displayed promising antibacterial properties against E. coli and S. aureus, with zones of inhibition ranging from 8.0 ± 0.0 to 11.7 ± 0.6 mm and 7.3 ± 0.6 to 9.7 ± 0.6 mm, respectively, as the concentration of c-AgNPs increased from 0.1 to 4 mg/mL.					
36677963	3	60	theme	present	563:569	arg1	groups					556:561	functional groups	545:561	functional groups present in the carrageenan	545:588	The FTIR spectra showed that the abundance of functional groups present in the carrageenan were responsible for the reduction and stabilisation of the c-AgNPs.					
36677963	2	61	theme	colour	356:361	arg1	change					363:368	the colour change	352:368	the colour change of the solution	352:384	The reduction of silver nitrate (AgNO3) to c-AgNPs was evident by the colour change of the solution from colourless to dark brown and further confirmed by a UV-Vis surface plasmon resonance (SPR) peak at ~420 nm.					
36677963	3	62	attach	present	563:569	arg2	groups					556:561	functional groups	545:561	functional groups present in the carrageenan	545:588	The FTIR spectra showed that the abundance of functional groups present in the carrageenan were responsible for the reduction and stabilisation of the c-AgNPs.					
36677963	3	62	attach	present	563:569	arg1	carrageenan					578:588	the carrageenan	574:588	the carrageenan	574:588	The FTIR spectra showed that the abundance of functional groups present in the carrageenan were responsible for the reduction and stabilisation of the c-AgNPs.					
36677963	7	63	theme	antibacterial	1186:1198	arg1	properties					1200:1209	promising antibacterial properties	1176:1209	promising antibacterial properties	1176:1209	The c-AgNPs displayed promising antibacterial properties against E. coli and S. aureus, with zones of inhibition ranging from 8.0 ± 0.0 to 11.7 ± 0.6 mm and 7.3 ± 0.6 to 9.7 ± 0.6 mm, respectively, as the concentration of c-AgNPs increased from 0.1 to 4 mg/mL.					
36677963	7	64	theme	c-AgNPs	1376:1382	arg1	concentration					1359:1371	the concentration	1355:1371	the concentration of c-AgNPs	1355:1382	The c-AgNPs displayed promising antibacterial properties against E. coli and S. aureus, with zones of inhibition ranging from 8.0 ± 0.0 to 11.7 ± 0.6 mm and 7.3 ± 0.6 to 9.7 ± 0.6 mm, respectively, as the concentration of c-AgNPs increased from 0.1 to 4 mg/mL.					
36677963	5	65	theme	flower-like	950:960	arg1	c-AgNPs					962:968	flower-like c-AgNPs	950:968	flower-like c-AgNPs composed of intercrossed and random lamellar petals of approximately 50 nm in thickness	950:1056	Interestingly, the morphological characterisations by SEM and FE-SEM revealed the formation of flower-like c-AgNPs composed of intercrossed and random lamellar petals of approximately 50 nm in thickness.					
36677963	7	66	dep	mg/mL	1408:1412	arg1	to					1403:1404	to	1403:1404	to	1403:1404	The c-AgNPs displayed promising antibacterial properties against E. coli and S. aureus, with zones of inhibition ranging from 8.0 ± 0.0 to 11.7 ± 0.6 mm and 7.3 ± 0.6 to 9.7 ± 0.6 mm, respectively, as the concentration of c-AgNPs increased from 0.1 to 4 mg/mL.					
36358088	0	0	theme	maleic	92:97	arg1	hydrolysis					104:113	ultrasound-assisted maleic acid hydrolysis	72:113	ultrasound-assisted maleic acid hydrolysis	72:113	Preparation of cellulose nanocrystals from purple sweet potato peels by ultrasound-assisted maleic acid hydrolysis.					
36358088	2	1	theme	antioxidant	345:355	arg1	activity					357:364	antioxidant activity	345:364	antioxidant activity	345:364	Firstly, ultrasonic assisted ethanol method was used to extract anthocyanins from PSP peels, and the individual anthocyanin composition and antioxidant activity were analyzed.					
36358088	0	2	theme	ultrasound-assisted	72:90	arg1	hydrolysis					104:113	ultrasound-assisted maleic acid hydrolysis	72:113	ultrasound-assisted maleic acid hydrolysis	72:113	Preparation of cellulose nanocrystals from purple sweet potato peels by ultrasound-assisted maleic acid hydrolysis.					
36358088	4	3	theme	peel	710:713	arg1	residues					715:722	PSP peel residues	706:722	PSP peel residues	706:722	Results showed the optimal technological parameters were as follows: the ratio between PSP peel residues and 75 wt% maleic acid was 1:10 (g/mL, W/W), and ultrasonic-assisted hydrolysis was carried out at 60℃ for 1 h, followed by 120℃ for 2.5 h.					
36358088	7	4	contain	has	1096:1098	arg1	hydrolysis					1085:1094	ultrasound-assisted maleic acid hydrolysis	1053:1094	ultrasound-assisted maleic acid hydrolysis	1053:1094	In conclusion, ultrasound-assisted maleic acid hydrolysis has great potential to realize the industrialization of CNCs.					
36358088	7	4	contain	has	1096:1098	arg2	potential					1106:1114	great potential	1100:1114	great potential	1100:1114	In conclusion, ultrasound-assisted maleic acid hydrolysis has great potential to realize the industrialization of CNCs.					
36358088	6	5	dep	structure	954:962	arg1	The					941:943	The	941:943	The	941:943	The chemical structure and physical properties of CNCs were similar to those of commercial CNCs.					
36358088	7	6	theme	maleic	1073:1078	arg1	hydrolysis					1085:1094	ultrasound-assisted maleic acid hydrolysis	1053:1094	ultrasound-assisted maleic acid hydrolysis	1053:1094	In conclusion, ultrasound-assisted maleic acid hydrolysis has great potential to realize the industrialization of CNCs.					
36358088	6	7	theme	chemical	945:952	arg1	structure					954:962	chemical structure	945:962	chemical structure	945:962	The chemical structure and physical properties of CNCs were similar to those of commercial CNCs.					
36358088	0	8	theme	acid	99:102	arg1	hydrolysis					104:113	ultrasound-assisted maleic acid hydrolysis	72:113	ultrasound-assisted maleic acid hydrolysis	72:113	Preparation of cellulose nanocrystals from purple sweet potato peels by ultrasound-assisted maleic acid hydrolysis.					
36358088	0	9	from	potato	56:61	arg1	Preparation					0:10	Preparation	0:10	Preparation of cellulose nanocrystals from purple sweet potato	0:61	Preparation of cellulose nanocrystals from purple sweet potato peels by ultrasound-assisted maleic acid hydrolysis.					
36358088	7	10	theme	acid	1080:1083	arg1	hydrolysis					1085:1094	ultrasound-assisted maleic acid hydrolysis	1053:1094	ultrasound-assisted maleic acid hydrolysis	1053:1094	In conclusion, ultrasound-assisted maleic acid hydrolysis has great potential to realize the industrialization of CNCs.					
36358088	3	11	theme	thermal	560:566	arg1	stability					568:576	thermal stability	560:576	thermal stability	560:576	Then the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis were optimized, and Zeta-potential, chemical structure, thermal stability and crystallinity of CNCs were analyzed.					
36358088	5	12	theme	CNCs	896:899	arg1	Zeta-potential					878:891	Zeta-potential	878:891	Zeta-potential	878:891	The yield and Zeta-potential of CNCs were 8.17 % and -57.7 mV, respectively.					
36358088	5	12	theme	CNCs	896:899	arg1	yield					868:872	yield	868:872	yield	868:872	The yield and Zeta-potential of CNCs were 8.17 % and -57.7 mV, respectively.					
36358088	4	13	theme	technological	646:658	arg1	parameters					660:669	the optimal technological parameters	634:669	the optimal technological parameters	634:669	Results showed the optimal technological parameters were as follows: the ratio between PSP peel residues and 75 wt% maleic acid was 1:10 (g/mL, W/W), and ultrasonic-assisted hydrolysis was carried out at 60℃ for 1 h, followed by 120℃ for 2.5 h.					
36358088	2	14	theme	extract	261:267	arg1	anthocyanins					269:280	extract anthocyanins	261:280	extract anthocyanins from PSP peels	261:295	Firstly, ultrasonic assisted ethanol method was used to extract anthocyanins from PSP peels, and the individual anthocyanin composition and antioxidant activity were analyzed.					
36358088	3	15	theme	cellulose	428:436	arg1	CNCs					452:455	CNCs	452:455	CNCs	452:455	Then the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis were optimized, and Zeta-potential, chemical structure, thermal stability and crystallinity of CNCs were analyzed.					
36358088	3	15	theme	cellulose	428:436	arg1	nanocrystals					438:449	cellulose nanocrystals	428:449	cellulose nanocrystals (CNCs)	428:456	Then the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis were optimized, and Zeta-potential, chemical structure, thermal stability and crystallinity of CNCs were analyzed.					
36358088	1	16	theme	purple	172:177	arg1	peels					198:202	purple sweet potato (PSP) peels	172:202	purple sweet potato (PSP) peels	172:202	This study aimed at realizing high value utilization of purple sweet potato (PSP) peels.					
36358088	7	17	theme	ultrasound-assisted	1053:1071	arg1	hydrolysis					1085:1094	ultrasound-assisted maleic acid hydrolysis	1053:1094	ultrasound-assisted maleic acid hydrolysis	1053:1094	In conclusion, ultrasound-assisted maleic acid hydrolysis has great potential to realize the industrialization of CNCs.					
36358088	1	18	theme	sweet	179:183	arg1	peels					198:202	purple sweet potato (PSP) peels	172:202	purple sweet potato (PSP) peels	172:202	This study aimed at realizing high value utilization of purple sweet potato (PSP) peels.					
36358088	2	19	used	used	253:256	arg2	method					242:247	ultrasonic assisted ethanol method	214:247	ultrasonic assisted ethanol method	214:247	Firstly, ultrasonic assisted ethanol method was used to extract anthocyanins from PSP peels, and the individual anthocyanin composition and antioxidant activity were analyzed.					
36358088	1	20	theme	potato	185:190	arg1	peels					198:202	purple sweet potato (PSP) peels	172:202	purple sweet potato (PSP) peels	172:202	This study aimed at realizing high value utilization of purple sweet potato (PSP) peels.					
36358088	7	21	theme	CNCs	1152:1155	arg1	industrialization					1131:1147	the industrialization	1127:1147	the industrialization of CNCs	1127:1155	In conclusion, ultrasound-assisted maleic acid hydrolysis has great potential to realize the industrialization of CNCs.					
36358088	5	22	dep	yield	868:872	arg1	The					864:866	The	864:866	The	864:866	The yield and Zeta-potential of CNCs were 8.17 % and -57.7 mV, respectively.					
36358088	0	23	theme	nanocrystals	25:36	arg1	Preparation					0:10	Preparation	0:10	Preparation of cellulose nanocrystals from purple sweet potato	0:61	Preparation of cellulose nanocrystals from purple sweet potato peels by ultrasound-assisted maleic acid hydrolysis.					
36358088	1	24	theme	PSP	193:195	arg1	peels					198:202	purple sweet potato (PSP) peels	172:202	purple sweet potato (PSP) peels	172:202	This study aimed at realizing high value utilization of purple sweet potato (PSP) peels.					
36358088	0	25	theme	cellulose	15:23	arg1	nanocrystals					25:36	cellulose nanocrystals	15:36	cellulose nanocrystals	15:36	Preparation of cellulose nanocrystals from purple sweet potato peels by ultrasound-assisted maleic acid hydrolysis.					
36358088	3	26	theme	chemical	540:547	arg1	structure					549:557	chemical structure	540:557	chemical structure	540:557	Then the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis were optimized, and Zeta-potential, chemical structure, thermal stability and crystallinity of CNCs were analyzed.					
36358088	2	27	theme	PSP	287:289	arg1	peels					291:295	PSP peels	287:295	PSP peels	287:295	Firstly, ultrasonic assisted ethanol method was used to extract anthocyanins from PSP peels, and the individual anthocyanin composition and antioxidant activity were analyzed.					
36358088	3	28	theme	maleic	481:486	arg1	hydrolysis					493:502	ultrasonic-assisted maleic acid hydrolysis	461:502	ultrasonic-assisted maleic acid hydrolysis	461:502	Then the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis were optimized, and Zeta-potential, chemical structure, thermal stability and crystallinity of CNCs were analyzed.					
36358088	0	29	theme	purple	43:48	arg1	potato					56:61	purple sweet potato	43:61	purple sweet potato	43:61	Preparation of cellulose nanocrystals from purple sweet potato peels by ultrasound-assisted maleic acid hydrolysis.					
36358088	4	30	dep	1:10	751:754	arg1	W/W					763:765	W/W	763:765	W/W	763:765	Results showed the optimal technological parameters were as follows: the ratio between PSP peel residues and 75 wt% maleic acid was 1:10 (g/mL, W/W), and ultrasonic-assisted hydrolysis was carried out at 60℃ for 1 h, followed by 120℃ for 2.5 h.					
36358088	4	30	dep	1:10	751:754	arg1	g/mL					757:760	g/mL	757:760	g/mL	757:760	Results showed the optimal technological parameters were as follows: the ratio between PSP peel residues and 75 wt% maleic acid was 1:10 (g/mL, W/W), and ultrasonic-assisted hydrolysis was carried out at 60℃ for 1 h, followed by 120℃ for 2.5 h.					
36358088	4	31	dep	carried	808:814	arg1	followed					836:843	followed	836:843	followed by 120℃ for 2.5 h	836:861	Results showed the optimal technological parameters were as follows: the ratio between PSP peel residues and 75 wt% maleic acid was 1:10 (g/mL, W/W), and ultrasonic-assisted hydrolysis was carried out at 60℃ for 1 h, followed by 120℃ for 2.5 h.					
36358088	2	32	theme	assisted	225:232	arg1	method					242:247	ultrasonic assisted ethanol method	214:247	ultrasonic assisted ethanol method	214:247	Firstly, ultrasonic assisted ethanol method was used to extract anthocyanins from PSP peels, and the individual anthocyanin composition and antioxidant activity were analyzed.					
36358088	1	33	theme	peels	198:202	arg1	utilization					157:167	high value utilization	146:167	high value utilization of purple sweet potato (PSP) peels	146:202	This study aimed at realizing high value utilization of purple sweet potato (PSP) peels.					
36358088	4	34	theme	PSP	706:708	arg1	residues					715:722	PSP peel residues	706:722	PSP peel residues	706:722	Results showed the optimal technological parameters were as follows: the ratio between PSP peel residues and 75 wt% maleic acid was 1:10 (g/mL, W/W), and ultrasonic-assisted hydrolysis was carried out at 60℃ for 1 h, followed by 120℃ for 2.5 h.					
36358088	2	35	theme	ultrasonic	214:223	arg1	method					242:247	ultrasonic assisted ethanol method	214:247	ultrasonic assisted ethanol method	214:247	Firstly, ultrasonic assisted ethanol method was used to extract anthocyanins from PSP peels, and the individual anthocyanin composition and antioxidant activity were analyzed.					
36358088	6	36	theme	commercial	1021:1030	arg1	CNCs					1032:1035	commercial CNCs	1021:1035	commercial CNCs	1021:1035	The chemical structure and physical properties of CNCs were similar to those of commercial CNCs.					
36358088	3	37	theme	technological	390:402	arg1	parameters					404:413	the technological parameters	386:413	the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis	386:502	Then the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis were optimized, and Zeta-potential, chemical structure, thermal stability and crystallinity of CNCs were analyzed.					
36358088	3	38	theme	CNCs	599:602	arg1	crystallinity					582:594	crystallinity	582:594	crystallinity	582:594	Then the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis were optimized, and Zeta-potential, chemical structure, thermal stability and crystallinity of CNCs were analyzed.					
36358088	3	38	theme	CNCs	599:602	arg1	Zeta-potential					524:537	Zeta-potential	524:537	Zeta-potential	524:537	Then the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis were optimized, and Zeta-potential, chemical structure, thermal stability and crystallinity of CNCs were analyzed.					
36358088	3	38	theme	CNCs	599:602	arg1	structure					549:557	chemical structure	540:557	chemical structure	540:557	Then the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis were optimized, and Zeta-potential, chemical structure, thermal stability and crystallinity of CNCs were analyzed.					
36358088	3	38	theme	CNCs	599:602	arg1	stability					568:576	thermal stability	560:576	thermal stability	560:576	Then the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis were optimized, and Zeta-potential, chemical structure, thermal stability and crystallinity of CNCs were analyzed.					
36358088	4	39	theme	75 wt	728:732	arg1	%					733:733	%	733:733	%	733:733	Results showed the optimal technological parameters were as follows: the ratio between PSP peel residues and 75 wt% maleic acid was 1:10 (g/mL, W/W), and ultrasonic-assisted hydrolysis was carried out at 60℃ for 1 h, followed by 120℃ for 2.5 h.					
36358088	0	40	theme	sweet	50:54	arg1	potato					56:61	purple sweet potato	43:61	purple sweet potato	43:61	Preparation of cellulose nanocrystals from purple sweet potato peels by ultrasound-assisted maleic acid hydrolysis.					
36358088	2	41	theme	anthocyanin	317:327	arg1	composition					329:339	the individual anthocyanin composition	302:339	the individual anthocyanin composition	302:339	Firstly, ultrasonic assisted ethanol method was used to extract anthocyanins from PSP peels, and the individual anthocyanin composition and antioxidant activity were analyzed.					
36358088	4	42	theme	ultrasonic-assisted	773:791	arg1	hydrolysis					793:802	ultrasonic-assisted hydrolysis	773:802	ultrasonic-assisted hydrolysis	773:802	Results showed the optimal technological parameters were as follows: the ratio between PSP peel residues and 75 wt% maleic acid was 1:10 (g/mL, W/W), and ultrasonic-assisted hydrolysis was carried out at 60℃ for 1 h, followed by 120℃ for 2.5 h.					
36358088	2	43	theme	individual	306:315	arg1	composition					329:339	the individual anthocyanin composition	302:339	the individual anthocyanin composition	302:339	Firstly, ultrasonic assisted ethanol method was used to extract anthocyanins from PSP peels, and the individual anthocyanin composition and antioxidant activity were analyzed.					
36358088	6	44	theme	CNCs	991:994	arg1	structure					954:962	chemical structure	945:962	chemical structure	945:962	The chemical structure and physical properties of CNCs were similar to those of commercial CNCs.					
36358088	6	44	theme	CNCs	991:994	arg1	properties					977:986	physical properties	968:986	physical properties	968:986	The chemical structure and physical properties of CNCs were similar to those of commercial CNCs.					
36358088	4	45	dep	showed	627:632	arg1	were					671:674	were	671:674	showed the optimal technological parameters were as follows	627:685	Results showed the optimal technological parameters were as follows: the ratio between PSP peel residues and 75 wt% maleic acid was 1:10 (g/mL, W/W), and ultrasonic-assisted hydrolysis was carried out at 60℃ for 1 h, followed by 120℃ for 2.5 h.					
36358088	4	46	theme	maleic	735:740	arg1	acid					742:745	75 wt% maleic acid	728:745	75 wt% maleic acid	728:745	Results showed the optimal technological parameters were as follows: the ratio between PSP peel residues and 75 wt% maleic acid was 1:10 (g/mL, W/W), and ultrasonic-assisted hydrolysis was carried out at 60℃ for 1 h, followed by 120℃ for 2.5 h.					
36358088	4	47	theme	%	733:733	arg1	acid					742:745	75 wt% maleic acid	728:745	75 wt% maleic acid	728:745	Results showed the optimal technological parameters were as follows: the ratio between PSP peel residues and 75 wt% maleic acid was 1:10 (g/mL, W/W), and ultrasonic-assisted hydrolysis was carried out at 60℃ for 1 h, followed by 120℃ for 2.5 h.					
36358088	3	48	theme	acid	488:491	arg1	hydrolysis					493:502	ultrasonic-assisted maleic acid hydrolysis	461:502	ultrasonic-assisted maleic acid hydrolysis	461:502	Then the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis were optimized, and Zeta-potential, chemical structure, thermal stability and crystallinity of CNCs were analyzed.					
36358088	3	49	theme	ultrasonic-assisted	461:479	arg1	hydrolysis					493:502	ultrasonic-assisted maleic acid hydrolysis	461:502	ultrasonic-assisted maleic acid hydrolysis	461:502	Then the technological parameters of preparing cellulose nanocrystals (CNCs) by ultrasonic-assisted maleic acid hydrolysis were optimized, and Zeta-potential, chemical structure, thermal stability and crystallinity of CNCs were analyzed.					
36358088	1	50	theme	high	146:149	arg1	utilization					157:167	high value utilization	146:167	high value utilization of purple sweet potato (PSP) peels	146:202	This study aimed at realizing high value utilization of purple sweet potato (PSP) peels.					
36358088	4	51	theme	optimal	638:644	arg1	parameters					660:669	the optimal technological parameters	634:669	the optimal technological parameters	634:669	Results showed the optimal technological parameters were as follows: the ratio between PSP peel residues and 75 wt% maleic acid was 1:10 (g/mL, W/W), and ultrasonic-assisted hydrolysis was carried out at 60℃ for 1 h, followed by 120℃ for 2.5 h.					
36358088	7	52	theme	great	1100:1104	arg1	potential					1106:1114	great potential	1100:1114	great potential	1100:1114	In conclusion, ultrasound-assisted maleic acid hydrolysis has great potential to realize the industrialization of CNCs.					
36358088	2	53	theme	ethanol	234:240	arg1	method					242:247	ultrasonic assisted ethanol method	214:247	ultrasonic assisted ethanol method	214:247	Firstly, ultrasonic assisted ethanol method was used to extract anthocyanins from PSP peels, and the individual anthocyanin composition and antioxidant activity were analyzed.					
36358088	1	54	theme	value	151:155	arg1	utilization					157:167	high value utilization	146:167	high value utilization of purple sweet potato (PSP) peels	146:202	This study aimed at realizing high value utilization of purple sweet potato (PSP) peels.					
36358088	2	55	from	peels	291:295	arg1	anthocyanins					269:280	extract anthocyanins	261:280	extract anthocyanins from PSP peels	261:295	Firstly, ultrasonic assisted ethanol method was used to extract anthocyanins from PSP peels, and the individual anthocyanin composition and antioxidant activity were analyzed.					
36358088	6	56	theme	physical	968:975	arg1	properties					977:986	physical properties	968:986	physical properties	968:986	The chemical structure and physical properties of CNCs were similar to those of commercial CNCs.					
36935187	4	0	theme	gel	1161:1163	arg1	networks					1165:1172	reversible gel networks	1150:1172	reversible gel networks	1150:1172	Chemical cross-linking is commonly employed to fabricate irreversible permanent chitosan gels, and physical cross-linking enables the formation of reversible gel networks.					
36935187	8	1	theme	combined	1780:1787	arg1	application					1789:1799	the combined application	1776:1799	the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies	1776:1914	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	2	theme	factors	1856:1862	arg1	biotechnologies					1900:1914	state-of-the-art biotechnologies	1883:1914	state-of-the-art biotechnologies	1883:1914	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	2	theme	factors	1856:1862	arg1	biomaterials					1865:1876	biomaterials	1865:1876	biomaterials	1865:1876	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	2	theme	factors	1856:1862	arg1	hydrogels					1819:1827	chitosan-based hydrogels	1804:1827	chitosan-based hydrogels	1804:1827	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	2	theme	factors	1856:1862	arg1	variety					1835:1841	a variety	1833:1841	a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies	1833:1914	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	2	theme	factors	1856:1862	arg1	factors					1856:1862	bioactive factors	1846:1862	bioactive factors	1846:1862	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	7	3	theme	multiple	1630:1637	arg1	challenges					1639:1648	multiple challenges	1630:1648	multiple challenges	1630:1648	However, the clinical translation of chitosan-based hydrogels still encounters multiple challenges.					
36935187	7	4	theme	clinical	1564:1571	arg1	translation					1573:1583	the clinical translation	1560:1583	the clinical translation of chitosan-based hydrogels	1560:1611	However, the clinical translation of chitosan-based hydrogels still encounters multiple challenges.					
36935187	6	5	theme	remarkable	1392:1401	arg1	advances					1403:1410	remarkable advances	1392:1410	remarkable advances	1392:1410	Currently, remarkable advances have been made in promoting the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage.					
36935187	8	6	theme	biomaterials	1865:1876	arg1	biotechnologies					1900:1914	state-of-the-art biotechnologies	1883:1914	state-of-the-art biotechnologies	1883:1914	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	6	theme	biomaterials	1865:1876	arg1	biomaterials					1865:1876	biomaterials	1865:1876	biomaterials	1865:1876	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	6	theme	biomaterials	1865:1876	arg1	hydrogels					1819:1827	chitosan-based hydrogels	1804:1827	chitosan-based hydrogels	1804:1827	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	6	theme	biomaterials	1865:1876	arg1	variety					1835:1841	a variety	1833:1841	a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies	1833:1914	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	6	theme	biomaterials	1865:1876	arg1	factors					1856:1862	bioactive factors	1846:1862	bioactive factors	1846:1862	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	3	7	theme	composite	774:782	arg1	systems					784:790	hydrogel composite systems	765:790	hydrogel composite systems	765:790	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	1	8	theme	maxillofacial	164:176	arg1	diseases					178:185	common oral and maxillofacial diseases	148:185	common oral and maxillofacial diseases in clinic	148:195	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	8	theme	maxillofacial	164:176	arg1	periodontitis					77:89	periodontitis	77:89	periodontitis	77:89	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	8	theme	maxillofacial	164:176	arg1	Pulpitis					67:74	Pulpitis	67:74	Pulpitis	67:74	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	8	theme	maxillofacial	164:176	arg1	damage					137:142	temporomandibular joint damage	113:142	temporomandibular joint damage	113:142	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	8	theme	maxillofacial	164:176	arg1	defect					101:106	jaw bone defect	92:106	jaw bone defect	92:106	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	7	9	theme	chitosan-based	1588:1601	arg1	hydrogels					1603:1611	chitosan-based hydrogels	1588:1611	chitosan-based hydrogels	1588:1611	However, the clinical translation of chitosan-based hydrogels still encounters multiple challenges.					
36935187	8	10	theme	biotechnologies	1900:1914	arg1	biotechnologies					1900:1914	state-of-the-art biotechnologies	1883:1914	state-of-the-art biotechnologies	1883:1914	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	10	theme	biotechnologies	1900:1914	arg1	biomaterials					1865:1876	biomaterials	1865:1876	biomaterials	1865:1876	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	10	theme	biotechnologies	1900:1914	arg1	hydrogels					1819:1827	chitosan-based hydrogels	1804:1827	chitosan-based hydrogels	1804:1827	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	10	theme	biotechnologies	1900:1914	arg1	variety					1835:1841	a variety	1833:1841	a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies	1833:1914	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	10	theme	biotechnologies	1900:1914	arg1	factors					1856:1862	bioactive factors	1846:1862	bioactive factors	1846:1862	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	3	11	theme	porous	805:810	arg1	microspheres					812:823	carrying porous microspheres	796:823	carrying porous microspheres	796:823	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	6	12	theme	pulp-dentin	1460:1470	arg1	complex					1472:1478	pulp-dentin complex	1460:1478	pulp-dentin complex	1460:1478	Currently, remarkable advances have been made in promoting the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage.					
36935187	5	13	theme	suitable	1188:1195	arg1	biomaterials					1206:1217	suitable scaffold biomaterials	1188:1217	suitable scaffold biomaterials	1188:1217	Representing suitable scaffold biomaterials, several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes have been used in an attempt to regenerate oral soft and hard tissues.					
36935187	8	14	theme	multifaceted	1951:1962	arg1	regeneration					1973:1984	multifaceted complete regeneration	1951:1984	multifaceted complete regeneration of oral tissue	1951:1999	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	3	15	theme	local	854:858	arg1	delivery					871:878	local sequential delivery	854:878	local sequential delivery of diverse drugs or bioactive factors	854:916	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	2	16	theme	chitosan-based	443:456	arg1	hydrogels					458:466	chitosan-based hydrogels	443:466	chitosan-based hydrogels	443:466	Due to their good biocompatibility, biodegradability, antioxidant effect, anti-inflammatory activity, and broad-spectrum antimicrobial property, chitosan-based hydrogels have shown broad applicable prospects in the field of oral tissue engineering.					
36935187	1	17	theme	traditional	202:212	arg1	treatments					214:223	traditional treatments	202:223	traditional treatments	202:223	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	3	18	theme	modification	619:630	arg1	strategies					632:641	chemical modification strategies	610:641	chemical modification strategies	610:641	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	3	19	theme	solid	928:932	arg1	foundation					934:943	a solid foundation	926:943	a solid foundation for the well-organized regeneration of defective tissues	926:1000	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	3	20	theme	diverse	883:889	arg1	drugs					891:895	diverse drugs	883:895	diverse drugs	883:895	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	1	21	from	diseases	178:185	arg1	clinic					190:195	clinic	190:195	clinic	190:195	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	22	theme	joint	131:135	arg1	diseases					178:185	common oral and maxillofacial diseases	148:185	common oral and maxillofacial diseases in clinic	148:195	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	22	theme	joint	131:135	arg1	periodontitis					77:89	periodontitis	77:89	periodontitis	77:89	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	22	theme	joint	131:135	arg1	Pulpitis					67:74	Pulpitis	67:74	Pulpitis	67:74	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	22	theme	joint	131:135	arg1	damage					137:142	temporomandibular joint damage	113:142	temporomandibular joint damage	113:142	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	22	theme	joint	131:135	arg1	defect					101:106	jaw bone defect	92:106	jaw bone defect	92:106	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	4	23	theme	chitosan	1083:1090	arg1	gels					1092:1095	irreversible permanent chitosan gels	1060:1095	irreversible permanent chitosan gels	1060:1095	Chemical cross-linking is commonly employed to fabricate irreversible permanent chitosan gels, and physical cross-linking enables the formation of reversible gel networks.					
36935187	6	24	theme	bone	1530:1533	arg1	regeneration					1444:1455	the regeneration	1440:1455	the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage	1440:1548	Currently, remarkable advances have been made in promoting the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage.					
36935187	3	25	theme	well-organized	953:966	arg1	regeneration					968:979	the well-organized regeneration	949:979	the well-organized regeneration of defective tissues	949:1000	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	3	26	theme	factors	910:916	arg1	delivery					871:878	local sequential delivery	854:878	local sequential delivery of diverse drugs or bioactive factors	854:916	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	8	27	theme	oral	1989:1992	arg1	tissue					1994:1999	oral tissue	1989:1999	oral tissue	1989:1999	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	1	28	theme	oral	155:158	arg1	diseases					178:185	common oral and maxillofacial diseases	148:185	common oral and maxillofacial diseases in clinic	148:195	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	28	theme	oral	155:158	arg1	periodontitis					77:89	periodontitis	77:89	periodontitis	77:89	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	28	theme	oral	155:158	arg1	Pulpitis					67:74	Pulpitis	67:74	Pulpitis	67:74	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	28	theme	oral	155:158	arg1	damage					137:142	temporomandibular joint damage	113:142	temporomandibular joint damage	113:142	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	28	theme	oral	155:158	arg1	defect					101:106	jaw bone defect	92:106	jaw bone defect	92:106	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	0	29	from	Applicatoin	0:10	arg1	engineering					54:64	oral tissue engineering	42:64	oral tissue engineering	42:64	Applicatoin of chitosan-based hydrogel in oral tissue engineering.					
36935187	6	30	theme	bone	1512:1515	arg1	complex					1517:1523	cementum-periodontium-alveolar bone complex	1481:1523	cementum-periodontium-alveolar bone complex	1481:1523	Currently, remarkable advances have been made in promoting the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage.					
36935187	5	31	theme	several	1220:1226	arg1	hydrogels					1243:1251	several chitosan-based hydrogels	1220:1251	several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes	1220:1308	Representing suitable scaffold biomaterials, several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes have been used in an attempt to regenerate oral soft and hard tissues.					
36935187	3	32	theme	tissues	994:1000	arg1	regeneration					968:979	the well-organized regeneration	949:979	the well-organized regeneration of defective tissues	949:1000	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	2	33	theme	tissue	527:532	arg1	engineering					534:544	oral tissue engineering	522:544	oral tissue engineering	522:544	Due to their good biocompatibility, biodegradability, antioxidant effect, anti-inflammatory activity, and broad-spectrum antimicrobial property, chitosan-based hydrogels have shown broad applicable prospects in the field of oral tissue engineering.					
36935187	8	34	theme	in	1667:1668	arg1	exploration					1684:1694	in vivo clinical exploration	1667:1694	more in vivo clinical exploration under the conditions of oral complex microenvironments	1662:1749	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	35	theme	clinical	1675:1682	arg1	exploration					1684:1694	in vivo clinical exploration	1667:1694	more in vivo clinical exploration under the conditions of oral complex microenvironments	1662:1749	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	2	36	theme	anti-inflammatory	372:388	arg1	activity					390:397	anti-inflammatory activity	372:397	anti-inflammatory activity	372:397	Due to their good biocompatibility, biodegradability, antioxidant effect, anti-inflammatory activity, and broad-spectrum antimicrobial property, chitosan-based hydrogels have shown broad applicable prospects in the field of oral tissue engineering.					
36935187	1	37	theme	jaw	92:94	arg1	diseases					178:185	common oral and maxillofacial diseases	148:185	common oral and maxillofacial diseases in clinic	148:195	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	37	theme	jaw	92:94	arg1	periodontitis					77:89	periodontitis	77:89	periodontitis	77:89	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	37	theme	jaw	92:94	arg1	Pulpitis					67:74	Pulpitis	67:74	Pulpitis	67:74	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	37	theme	jaw	92:94	arg1	damage					137:142	temporomandibular joint damage	113:142	temporomandibular joint damage	113:142	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	37	theme	jaw	92:94	arg1	defect					101:106	jaw bone defect	92:106	jaw bone defect	92:106	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	4	38	theme	reversible	1150:1159	arg1	networks					1165:1172	reversible gel networks	1150:1172	reversible gel networks	1150:1172	Chemical cross-linking is commonly employed to fabricate irreversible permanent chitosan gels, and physical cross-linking enables the formation of reversible gel networks.					
36935187	0	39	theme	chitosan-based	15:28	arg1	hydrogel					30:37	chitosan-based hydrogel	15:37	chitosan-based hydrogel	15:37	Applicatoin of chitosan-based hydrogel in oral tissue engineering.					
36935187	4	40	theme	physical	1102:1109	arg1	cross-linking					1111:1123	physical cross-linking	1102:1123	physical cross-linking	1102:1123	Chemical cross-linking is commonly employed to fabricate irreversible permanent chitosan gels, and physical cross-linking enables the formation of reversible gel networks.					
36935187	2	41	theme	broad-spectrum	404:417	arg1	property					433:440	broad-spectrum antimicrobial property	404:440	broad-spectrum antimicrobial property	404:440	Due to their good biocompatibility, biodegradability, antioxidant effect, anti-inflammatory activity, and broad-spectrum antimicrobial property, chitosan-based hydrogels have shown broad applicable prospects in the field of oral tissue engineering.					
36935187	1	42	theme	tissues	289:295	arg1	function					265:272	function	265:272	function	265:272	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	42	theme	tissues	289:295	arg1	structure					251:259	structure	251:259	structure	251:259	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	2	43	theme	broad	479:483	arg1	prospects					496:504	broad applicable prospects	479:504	broad applicable prospects	479:504	Due to their good biocompatibility, biodegradability, antioxidant effect, anti-inflammatory activity, and broad-spectrum antimicrobial property, chitosan-based hydrogels have shown broad applicable prospects in the field of oral tissue engineering.					
36935187	5	44	theme	growth	1283:1288	arg1	factors					1290:1296	growth factors	1283:1296	growth factors	1283:1296	Representing suitable scaffold biomaterials, several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes have been used in an attempt to regenerate oral soft and hard tissues.					
36935187	3	45	theme	biological	689:698	arg1	functions					700:708	biological functions	689:708	biological functions	689:708	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	0	46	theme	tissue	47:52	arg1	engineering					54:64	oral tissue engineering	42:64	oral tissue engineering	42:64	Applicatoin of chitosan-based hydrogel in oral tissue engineering.					
36935187	8	47	theme	microenvironments	1733:1749	arg1	conditions					1706:1715	the conditions	1702:1715	the conditions of oral complex microenvironments	1702:1749	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	5	48	used	used	1320:1323	arg2	hydrogels					1243:1251	several chitosan-based hydrogels	1220:1251	several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes	1220:1308	Representing suitable scaffold biomaterials, several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes have been used in an attempt to regenerate oral soft and hard tissues.					
36935187	2	49	theme	good	311:314	arg1	biocompatibility					316:331	their good biocompatibility	305:331	their good biocompatibility	305:331	Due to their good biocompatibility, biodegradability, antioxidant effect, anti-inflammatory activity, and broad-spectrum antimicrobial property, chitosan-based hydrogels have shown broad applicable prospects in the field of oral tissue engineering.					
36935187	8	50	theme	chitosan-based	1804:1817	arg1	hydrogels					1819:1827	chitosan-based hydrogels	1804:1827	chitosan-based hydrogels	1804:1827	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	5	51	theme	oral	1353:1356	arg1	tissues					1372:1378	oral soft and hard tissues	1353:1378	oral soft and hard tissues	1353:1378	Representing suitable scaffold biomaterials, several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes have been used in an attempt to regenerate oral soft and hard tissues.					
36935187	3	52	theme	hydrogels	728:736	arg1	functions					700:708	biological functions	689:708	biological functions	689:708	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	3	52	theme	hydrogels	728:736	arg1	properties					674:683	physicochemical properties	658:683	physicochemical properties	658:683	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	4	53	theme	networks	1165:1172	arg1	formation					1137:1145	the formation	1133:1145	the formation of reversible gel networks	1133:1172	Chemical cross-linking is commonly employed to fabricate irreversible permanent chitosan gels, and physical cross-linking enables the formation of reversible gel networks.					
36935187	8	54	theme	oral	1720:1723	arg1	microenvironments					1733:1749	oral complex microenvironments	1720:1749	oral complex microenvironments	1720:1749	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	3	55	theme	hydrogel	765:772	arg1	systems					784:790	hydrogel composite systems	765:790	hydrogel composite systems	765:790	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	8	56	theme	variety	1835:1841	arg1	application					1789:1799	the combined application	1776:1799	the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies	1776:1914	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	57	dep	more	1662:1665	arg1	exploration					1684:1694	in vivo clinical exploration	1667:1694	more in vivo clinical exploration under the conditions of oral complex microenvironments	1662:1749	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	8	58	theme	bioactive	1846:1854	arg1	factors					1856:1862	bioactive factors	1846:1862	bioactive factors	1846:1862	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	7	59	theme	hydrogels	1603:1611	arg1	translation					1573:1583	the clinical translation	1560:1583	the clinical translation of chitosan-based hydrogels	1560:1611	However, the clinical translation of chitosan-based hydrogels still encounters multiple challenges.					
36935187	3	60	theme	carrying	796:803	arg1	microspheres					812:823	carrying porous microspheres	796:823	carrying porous microspheres	796:823	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	3	61	theme	systems	784:790	arg1	construction					749:760	the construction	745:760	the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles	745:840	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	6	62	theme	complex	1472:1478	arg1	regeneration					1444:1455	the regeneration	1440:1455	the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage	1440:1548	Currently, remarkable advances have been made in promoting the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage.					
36935187	8	63	theme	state-of-the-art	1883:1898	arg1	biotechnologies					1900:1914	state-of-the-art biotechnologies	1883:1914	state-of-the-art biotechnologies	1883:1914	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	4	64	theme	Chemical	1003:1010	arg1	cross-linking					1012:1024	Chemical cross-linking	1003:1024	Chemical cross-linking	1003:1024	Chemical cross-linking is commonly employed to fabricate irreversible permanent chitosan gels, and physical cross-linking enables the formation of reversible gel networks.					
36935187	3	65	dep	common	603:608	arg1	improve					646:652	improve	646:652	to improve the physicochemical properties and biological functions of chitosan-based hydrogels	643:736	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	5	66	theme	scaffold	1197:1204	arg1	biomaterials					1206:1217	suitable scaffold biomaterials	1188:1217	suitable scaffold biomaterials	1188:1217	Representing suitable scaffold biomaterials, several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes have been used in an attempt to regenerate oral soft and hard tissues.					
36935187	3	67	theme	sequential	860:869	arg1	delivery					871:878	local sequential delivery	854:878	local sequential delivery of diverse drugs or bioactive factors	854:916	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	3	68	theme	chemical	610:617	arg1	strategies					632:641	chemical modification strategies	610:641	chemical modification strategies	610:641	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	1	69	dep	structure	251:259	arg1	the					247:249	the	247:249	the	247:249	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	3	70	theme	physicochemical	658:672	arg1	properties					674:683	physicochemical properties	658:683	physicochemical properties	658:683	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	1	71	theme	temporomandibular	113:129	arg1	diseases					178:185	common oral and maxillofacial diseases	148:185	common oral and maxillofacial diseases in clinic	148:195	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	71	theme	temporomandibular	113:129	arg1	periodontitis					77:89	periodontitis	77:89	periodontitis	77:89	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	71	theme	temporomandibular	113:129	arg1	Pulpitis					67:74	Pulpitis	67:74	Pulpitis	67:74	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	71	theme	temporomandibular	113:129	arg1	damage					137:142	temporomandibular joint damage	113:142	temporomandibular joint damage	113:142	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	71	theme	temporomandibular	113:129	arg1	defect					101:106	jaw bone defect	92:106	jaw bone defect	92:106	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	3	72	theme	drugs	891:895	arg1	delivery					871:878	local sequential delivery	854:878	local sequential delivery of diverse drugs or bioactive factors	854:916	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	4	73	theme	permanent	1073:1081	arg1	gels					1092:1095	irreversible permanent chitosan gels	1060:1095	irreversible permanent chitosan gels	1060:1095	Chemical cross-linking is commonly employed to fabricate irreversible permanent chitosan gels, and physical cross-linking enables the formation of reversible gel networks.					
36935187	6	74	theme	jaw	1526:1528	arg1	bone					1530:1533	jaw bone	1526:1533	jaw bone	1526:1533	Currently, remarkable advances have been made in promoting the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage.					
36935187	5	75	theme	stem	1271:1274	arg1	cells					1276:1280	stem cells	1271:1280	stem cells	1271:1280	Representing suitable scaffold biomaterials, several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes have been used in an attempt to regenerate oral soft and hard tissues.					
36935187	8	76	theme	complete	1964:1971	arg1	regeneration					1973:1984	multifaceted complete regeneration	1951:1984	multifaceted complete regeneration of oral tissue	1951:1999	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	1	77	theme	common	148:153	arg1	diseases					178:185	common oral and maxillofacial diseases	148:185	common oral and maxillofacial diseases in clinic	148:195	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	77	theme	common	148:153	arg1	periodontitis					77:89	periodontitis	77:89	periodontitis	77:89	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	77	theme	common	148:153	arg1	Pulpitis					67:74	Pulpitis	67:74	Pulpitis	67:74	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	77	theme	common	148:153	arg1	damage					137:142	temporomandibular joint damage	113:142	temporomandibular joint damage	113:142	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	77	theme	common	148:153	arg1	defect					101:106	jaw bone defect	92:106	jaw bone defect	92:106	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	6	78	theme	complex	1517:1523	arg1	regeneration					1444:1455	the regeneration	1440:1455	the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage	1440:1548	Currently, remarkable advances have been made in promoting the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage.					
36935187	2	79	theme	engineering	534:544	arg1	field					513:517	the field	509:517	the field of oral tissue engineering	509:544	Due to their good biocompatibility, biodegradability, antioxidant effect, anti-inflammatory activity, and broad-spectrum antimicrobial property, chitosan-based hydrogels have shown broad applicable prospects in the field of oral tissue engineering.					
36935187	3	80	theme	bioactive	900:908	arg1	factors					910:916	bioactive factors	900:916	bioactive factors	900:916	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	6	81	theme	cementum-periodontium-alveolar	1481:1510	arg1	complex					1517:1523	cementum-periodontium-alveolar bone complex	1481:1523	cementum-periodontium-alveolar bone complex	1481:1523	Currently, remarkable advances have been made in promoting the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage.					
36935187	5	82	theme	chitosan-based	1228:1241	arg1	hydrogels					1243:1251	several chitosan-based hydrogels	1220:1251	several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes	1220:1308	Representing suitable scaffold biomaterials, several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes have been used in an attempt to regenerate oral soft and hard tissues.					
36935187	8	83	theme	tissue	1994:1999	arg1	regeneration					1973:1984	multifaceted complete regeneration	1951:1984	multifaceted complete regeneration of oral tissue	1951:1999	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	2	84	theme	oral	522:525	arg1	engineering					534:544	oral tissue engineering	522:544	oral tissue engineering	522:544	Due to their good biocompatibility, biodegradability, antioxidant effect, anti-inflammatory activity, and broad-spectrum antimicrobial property, chitosan-based hydrogels have shown broad applicable prospects in the field of oral tissue engineering.					
36935187	4	85	theme	irreversible	1060:1071	arg1	gels					1092:1095	irreversible permanent chitosan gels	1060:1095	irreversible permanent chitosan gels	1060:1095	Chemical cross-linking is commonly employed to fabricate irreversible permanent chitosan gels, and physical cross-linking enables the formation of reversible gel networks.					
36935187	3	86	theme	defective	984:992	arg1	tissues					994:1000	defective tissues	984:1000	defective tissues	984:1000	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	0	87	theme	hydrogel	30:37	arg1	Applicatoin					0:10	Applicatoin	0:10	Applicatoin of chitosan-based hydrogel in oral tissue engineering.	0:65	Applicatoin of chitosan-based hydrogel in oral tissue engineering.					
36935187	1	88	theme	bone	96:99	arg1	diseases					178:185	common oral and maxillofacial diseases	148:185	common oral and maxillofacial diseases in clinic	148:195	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	88	theme	bone	96:99	arg1	periodontitis					77:89	periodontitis	77:89	periodontitis	77:89	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	88	theme	bone	96:99	arg1	Pulpitis					67:74	Pulpitis	67:74	Pulpitis	67:74	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	88	theme	bone	96:99	arg1	damage					137:142	temporomandibular joint damage	113:142	temporomandibular joint damage	113:142	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	1	88	theme	bone	96:99	arg1	defect					101:106	jaw bone defect	92:106	jaw bone defect	92:106	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	2	89	theme	antimicrobial	419:431	arg1	property					433:440	broad-spectrum antimicrobial property	404:440	broad-spectrum antimicrobial property	404:440	Due to their good biocompatibility, biodegradability, antioxidant effect, anti-inflammatory activity, and broad-spectrum antimicrobial property, chitosan-based hydrogels have shown broad applicable prospects in the field of oral tissue engineering.					
36935187	0	90	theme	oral	42:45	arg1	engineering					54:64	oral tissue engineering	42:64	oral tissue engineering	42:64	Applicatoin of chitosan-based hydrogel in oral tissue engineering.					
36935187	1	91	theme	injured	281:287	arg1	tissues					289:295	the injured tissues	277:295	the injured tissues	277:295	Pulpitis, periodontitis, jaw bone defect, and temporomandibular joint damage are common oral and maxillofacial diseases in clinic, but traditional treatments are unable to restore the structure and function of the injured tissues.					
36935187	2	92	theme	applicable	485:494	arg1	prospects					496:504	broad applicable prospects	479:504	broad applicable prospects	479:504	Due to their good biocompatibility, biodegradability, antioxidant effect, anti-inflammatory activity, and broad-spectrum antimicrobial property, chitosan-based hydrogels have shown broad applicable prospects in the field of oral tissue engineering.					
36935187	8	93	theme	complex	1725:1731	arg1	microenvironments					1733:1749	oral complex microenvironments	1720:1749	oral complex microenvironments	1720:1749	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	6	94	theme	cartilage	1540:1548	arg1	regeneration					1444:1455	the regeneration	1440:1455	the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage	1440:1548	Currently, remarkable advances have been made in promoting the regeneration of pulp-dentin complex, cementum-periodontium-alveolar bone complex, jaw bone, and cartilage.					
36935187	8	95	theme	hydrogels	1819:1827	arg1	application					1789:1799	the combined application	1776:1799	the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies	1776:1914	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	3	96	theme	chitosan-based	713:726	arg1	hydrogels					728:736	chitosan-based hydrogels	713:736	chitosan-based hydrogels	713:736	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	5	97	theme	soft	1358:1361	arg1	tissues					1372:1378	oral soft and hard tissues	1353:1378	oral soft and hard tissues	1353:1378	Representing suitable scaffold biomaterials, several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes have been used in an attempt to regenerate oral soft and hard tissues.					
36935187	3	98	dep	properties	674:683	arg1	the					654:656	the	654:656	the	654:656	Quaternization, carboxymethylation, and sulfonation are common chemical modification strategies to improve the physicochemical properties and biological functions of chitosan-based hydrogels, while the construction of hydrogel composite systems via carrying porous microspheres or nanoparticles can achieve local sequential delivery of diverse drugs or bioactive factors, laying a solid foundation for the well-organized regeneration of defective tissues.					
36935187	8	99	dep	in	1667:1668	arg1	vivo					1670:1673	vivo	1670:1673	vivo	1670:1673	In future, more in vivo clinical exploration under the conditions of oral complex microenvironments should be performed, and the combined application of chitosan-based hydrogels and a variety of bioactive factors, biomaterials, and state-of-the-art biotechnologies can be pursued in order to realize multifaceted complete regeneration of oral tissue.					
36935187	2	100	theme	antioxidant	352:362	arg1	effect					364:369	antioxidant effect	352:369	antioxidant effect	352:369	Due to their good biocompatibility, biodegradability, antioxidant effect, anti-inflammatory activity, and broad-spectrum antimicrobial property, chitosan-based hydrogels have shown broad applicable prospects in the field of oral tissue engineering.					
36935187	5	101	theme	hard	1367:1370	arg1	tissues					1372:1378	oral soft and hard tissues	1353:1378	oral soft and hard tissues	1353:1378	Representing suitable scaffold biomaterials, several chitosan-based hydrogels transplanted with stem cells, growth factors or exosomes have been used in an attempt to regenerate oral soft and hard tissues.					
35700848	0	0	theme	synergistic	111:121	arg1	effect					137:142	synergistic antibacterial effect	111:142	synergistic antibacterial effect	111:142	Curcumin-loaded HKUST-1@ carboxymethyl starch-based composites with moisture-responsive release properties and synergistic antibacterial effect for perishable fruits.					
35700848	3	1	theme	carboxymethyl	515:527	arg1	alcohol					552:558	carboxymethyl starch (CMS)/polyvinyl alcohol	515:558	carboxymethyl starch (CMS)/polyvinyl alcohol (PVA)	515:564	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	3	1	theme	carboxymethyl	515:527	arg1	PVA					561:563	PVA	561:563	PVA	561:563	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	9	2	theme	active	1413:1418	arg1	CMS/PVA					1390:1396	Cur-HKUST-1@CMS/PVA	1378:1396	Cur-HKUST-1@CMS/PVA	1378:1396	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	9	2	theme	active	1413:1418	arg1	material					1430:1437	a promising active packaging material	1401:1437	a promising active packaging material for improve the shelf life of perishable fruits	1401:1485	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	6	3	theme	58.32 	1051:1056	arg1	%					1046:1046	%	1046:1046	%	1046:1046	XRD and SEM demonstrated that moisture disrupts the structure of HKUST-1 and releases curcumin and the results showed that the release of curcumin increased from 25.11 % to 58.32 % after moisture stimulation.					
35700848	7	4	theme	@	1111:1111	arg1	CMS/PVA					1112:1118	Cur-HKUST-1@CMS/PVA	1100:1118	Cur-HKUST-1@CMS/PVA	1100:1118	In addition, Cur-HKUST-1@CMS/PVA had excellent antibacterial activity and antioxidant ability.					
35700848	8	5	theme	pitaya	1215:1220	arg1	freshness					1234:1242	pitaya and avocado freshness	1215:1242	pitaya and avocado freshness at least 4 days longer than the control	1215:1282	As validation, the film can keep pitaya and avocado freshness at least 4 days longer than the control, confirming the effectiveness of Cur-HKUST-1@CMS/PVA in preventing fruit decay.					
35700848	3	6	theme	starch	529:534	arg1	alcohol					552:558	carboxymethyl starch (CMS)/polyvinyl alcohol	515:558	carboxymethyl starch (CMS)/polyvinyl alcohol (PVA)	515:564	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	3	6	theme	starch	529:534	arg1	PVA					561:563	PVA	561:563	PVA	561:563	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	7	7	contain	had	1120:1122	arg2	ability					1173:1179	antioxidant ability	1161:1179	antioxidant ability	1161:1179	In addition, Cur-HKUST-1@CMS/PVA had excellent antibacterial activity and antioxidant ability.					
35700848	7	7	contain	had	1120:1122	arg1	CMS/PVA					1112:1118	Cur-HKUST-1@CMS/PVA	1100:1118	Cur-HKUST-1@CMS/PVA	1100:1118	In addition, Cur-HKUST-1@CMS/PVA had excellent antibacterial activity and antioxidant ability.					
35700848	7	7	contain	had	1120:1122	arg2	activity					1148:1155	excellent antibacterial activity	1124:1155	excellent antibacterial activity	1124:1155	In addition, Cur-HKUST-1@CMS/PVA had excellent antibacterial activity and antioxidant ability.					
35700848	9	8	theme	promising	1403:1411	arg1	CMS/PVA					1390:1396	Cur-HKUST-1@CMS/PVA	1378:1396	Cur-HKUST-1@CMS/PVA	1378:1396	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	9	8	theme	promising	1403:1411	arg1	material					1430:1437	a promising active packaging material	1401:1437	a promising active packaging material for improve the shelf life of perishable fruits	1401:1485	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	7	9	theme	Cur-HKUST-1	1100:1110	arg1	CMS/PVA					1112:1118	Cur-HKUST-1@CMS/PVA	1100:1118	Cur-HKUST-1@CMS/PVA	1100:1118	In addition, Cur-HKUST-1@CMS/PVA had excellent antibacterial activity and antioxidant ability.					
35700848	9	10	dep	material	1430:1437	arg1	improve					1443:1449	improve	1443:1449	improve the shelf life of perishable fruits	1443:1485	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	6	11	dep	%	1046:1046	arg1	to					1048:1049	to	1048:1049	to	1048:1049	XRD and SEM demonstrated that moisture disrupts the structure of HKUST-1 and releases curcumin and the results showed that the release of curcumin increased from 25.11 % to 58.32 % after moisture stimulation.					
35700848	3	12	theme	Cur-HKUST-1	406:416	arg1	film					400:403	a biological multifunctional film	371:403	a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment	371:482	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	3	12	theme	Cur-HKUST-1	406:416	arg1	CMS/PVA					418:424	Cur-HKUST-1@CMS/PVA	406:424	Cur-HKUST-1@CMS/PVA	406:424	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	0	13	theme	antibacterial	123:135	arg1	effect					137:142	synergistic antibacterial effect	111:142	synergistic antibacterial effect	111:142	Curcumin-loaded HKUST-1@ carboxymethyl starch-based composites with moisture-responsive release properties and synergistic antibacterial effect for perishable fruits.					
35700848	5	14	with	antibacterial	793:805	arg1	curcumin					812:819	curcumin	812:819	curcumin	812:819	HKUST-1 not only acts as curcumin carriers but also forms synergistic antibacterial with curcumin to improve the antibacterial activity of the composites.					
35700848	8	15	theme	CMS/PVA	1329:1335	arg1	effectiveness					1300:1312	the effectiveness	1296:1312	the effectiveness of Cur-HKUST-1@CMS/PVA in preventing fruit decay	1296:1361	As validation, the film can keep pitaya and avocado freshness at least 4 days longer than the control, confirming the effectiveness of Cur-HKUST-1@CMS/PVA in preventing fruit decay.					
35700848	4	16	theme	curcumin	692:699	arg1	release					701:707	curcumin release	692:707	curcumin release from HKUST-1	692:720	The hydrophilic CMS facilitates water adsorption and moisture can stimulate curcumin release from HKUST-1.					
35700848	9	17	theme	shelf	1455:1459	arg1	life					1461:1464	the shelf life	1451:1464	the shelf life of perishable fruits	1451:1485	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	9	18	theme	packaging	1420:1428	arg1	CMS/PVA					1390:1396	Cur-HKUST-1@CMS/PVA	1378:1396	Cur-HKUST-1@CMS/PVA	1378:1396	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	9	18	theme	packaging	1420:1428	arg1	material					1430:1437	a promising active packaging material	1401:1437	a promising active packaging material for improve the shelf life of perishable fruits	1401:1485	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	0	19	theme	perishable	148:157	arg1	fruits					159:164	perishable fruits	148:164	perishable fruits	148:164	Curcumin-loaded HKUST-1@ carboxymethyl starch-based composites with moisture-responsive release properties and synergistic antibacterial effect for perishable fruits.					
35700848	9	20	theme	fruits	1480:1485	arg1	life					1461:1464	the shelf life	1451:1464	the shelf life of perishable fruits	1451:1485	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	5	21	theme	antibacterial	836:848	arg1	activity					850:857	the antibacterial activity	832:857	the antibacterial activity of the composites	832:875	HKUST-1 not only acts as curcumin carriers but also forms synergistic antibacterial with curcumin to improve the antibacterial activity of the composites.					
35700848	2	22	theme	bacterial	291:299	arg1	reproduction					301:312	bacterial reproduction	291:312	bacterial reproduction	291:312	High humidity by fresh fruit respiration leads to bacterial reproduction, which is the key factor of products corruption.					
35700848	2	22	theme	bacterial	291:299	arg1	factor					332:337	the key factor	324:337	the key factor of products corruption	324:360	High humidity by fresh fruit respiration leads to bacterial reproduction, which is the key factor of products corruption.					
35700848	4	23	theme	water	648:652	arg1	adsorption					654:663	water adsorption	648:663	water adsorption	648:663	The hydrophilic CMS facilitates water adsorption and moisture can stimulate curcumin release from HKUST-1.					
35700848	7	24	theme	excellent	1124:1132	arg1	activity					1148:1155	excellent antibacterial activity	1124:1155	excellent antibacterial activity	1124:1155	In addition, Cur-HKUST-1@CMS/PVA had excellent antibacterial activity and antioxidant ability.					
35700848	3	25	theme	/polyvinyl	541:550	arg1	alcohol					552:558	carboxymethyl starch (CMS)/polyvinyl alcohol	515:558	carboxymethyl starch (CMS)/polyvinyl alcohol (PVA)	515:564	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	3	25	theme	/polyvinyl	541:550	arg1	PVA					561:563	PVA	561:563	PVA	561:563	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	5	26	theme	composites	866:875	arg1	activity					850:857	the antibacterial activity	832:857	the antibacterial activity of the composites	832:875	HKUST-1 not only acts as curcumin carriers but also forms synergistic antibacterial with curcumin to improve the antibacterial activity of the composites.					
35700848	0	27	theme	HKUST-1	16:22	arg1	carboxymethyl					25:37	Curcumin-loaded HKUST-1@ carboxymethyl	0:37	Curcumin-loaded HKUST-1@ carboxymethyl	0:37	Curcumin-loaded HKUST-1@ carboxymethyl starch-based composites with moisture-responsive release properties and synergistic antibacterial effect for perishable fruits.					
35700848	2	28	theme	key	328:330	arg1	reproduction					301:312	bacterial reproduction	291:312	bacterial reproduction	291:312	High humidity by fresh fruit respiration leads to bacterial reproduction, which is the key factor of products corruption.					
35700848	2	28	theme	key	328:330	arg1	factor					332:337	the key factor	324:337	the key factor of products corruption	324:360	High humidity by fresh fruit respiration leads to bacterial reproduction, which is the key factor of products corruption.					
35700848	0	29	theme	Curcumin-loaded	0:14	arg1	carboxymethyl					25:37	Curcumin-loaded HKUST-1@ carboxymethyl	0:37	Curcumin-loaded HKUST-1@ carboxymethyl	0:37	Curcumin-loaded HKUST-1@ carboxymethyl starch-based composites with moisture-responsive release properties and synergistic antibacterial effect for perishable fruits.					
35700848	8	30	dep	freshness	1234:1242	arg1	4 days					1253:1258	4 days	1253:1258	4 days	1253:1258	As validation, the film can keep pitaya and avocado freshness at least 4 days longer than the control, confirming the effectiveness of Cur-HKUST-1@CMS/PVA in preventing fruit decay.					
35700848	1	31	theme	important	208:216	arg1	causes					218:223	the most important causes	199:223	the most important causes of fruit waste	199:238	The spoilage of fruit is one of the most important causes of fruit waste.					
35700848	3	32	theme	biological	373:382	arg1	film					400:403	a biological multifunctional film	371:403	a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment	371:482	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	3	32	theme	biological	373:382	arg1	CMS/PVA					418:424	Cur-HKUST-1@CMS/PVA	406:424	Cur-HKUST-1@CMS/PVA	406:424	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	5	33	theme	synergistic	781:791	arg1	HKUST-1					723:729	HKUST-1	723:729	HKUST-1	723:729	HKUST-1 not only acts as curcumin carriers but also forms synergistic antibacterial with curcumin to improve the antibacterial activity of the composites.					
35700848	5	33	theme	synergistic	781:791	arg1	antibacterial					793:805	synergistic antibacterial	781:805	synergistic antibacterial with curcumin	781:819	HKUST-1 not only acts as curcumin carriers but also forms synergistic antibacterial with curcumin to improve the antibacterial activity of the composites.					
35700848	5	33	theme	synergistic	781:791	arg1	carriers					757:764	curcumin carriers	748:764	curcumin carriers	748:764	HKUST-1 not only acts as curcumin carriers but also forms synergistic antibacterial with curcumin to improve the antibacterial activity of the composites.					
35700848	1	34	theme	causes	218:223	arg1	spoilage					171:178	The spoilage	167:178	The spoilage of fruit	167:187	The spoilage of fruit is one of the most important causes of fruit waste.					
35700848	1	34	theme	causes	218:223	arg1	causes					218:223	the most important causes	199:223	the most important causes of fruit waste	199:238	The spoilage of fruit is one of the most important causes of fruit waste.					
35700848	1	34	theme	causes	218:223	arg1	one					192:194	one	192:194	one	192:194	The spoilage of fruit is one of the most important causes of fruit waste.					
35700848	0	35	theme	@	23:23	arg1	carboxymethyl					25:37	Curcumin-loaded HKUST-1@ carboxymethyl	0:37	Curcumin-loaded HKUST-1@ carboxymethyl	0:37	Curcumin-loaded HKUST-1@ carboxymethyl starch-based composites with moisture-responsive release properties and synergistic antibacterial effect for perishable fruits.					
35700848	8	36	theme	fruit	1351:1355	arg1	decay					1357:1361	fruit decay	1351:1361	fruit decay	1351:1361	As validation, the film can keep pitaya and avocado freshness at least 4 days longer than the control, confirming the effectiveness of Cur-HKUST-1@CMS/PVA in preventing fruit decay.					
35700848	8	37	theme	Cur-HKUST-1	1317:1327	arg1	CMS/PVA					1329:1335	Cur-HKUST-1@CMS/PVA	1317:1335	Cur-HKUST-1@CMS/PVA	1317:1335	As validation, the film can keep pitaya and avocado freshness at least 4 days longer than the control, confirming the effectiveness of Cur-HKUST-1@CMS/PVA in preventing fruit decay.					
35700848	3	38	theme	multifunctional	384:398	arg1	film					400:403	a biological multifunctional film	371:403	a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment	371:482	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	3	38	theme	multifunctional	384:398	arg1	CMS/PVA					418:424	Cur-HKUST-1@CMS/PVA	406:424	Cur-HKUST-1@CMS/PVA	406:424	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	4	39	theme	hydrophilic	620:630	arg1	CMS					632:634	The hydrophilic CMS	616:634	The hydrophilic CMS	616:634	The hydrophilic CMS facilitates water adsorption and moisture can stimulate curcumin release from HKUST-1.					
35700848	3	40	theme	fruits	431:436	arg1	preservation					438:449	fruits preservation	431:449	fruits preservation with a high moisture environment	431:482	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	1	41	theme	fruit	228:232	arg1	waste					234:238	fruit waste	228:238	fruit waste	228:238	The spoilage of fruit is one of the most important causes of fruit waste.					
35700848	8	42	theme	avocado	1226:1232	arg1	freshness					1234:1242	pitaya and avocado freshness	1215:1242	pitaya and avocado freshness at least 4 days longer than the control	1215:1282	As validation, the film can keep pitaya and avocado freshness at least 4 days longer than the control, confirming the effectiveness of Cur-HKUST-1@CMS/PVA in preventing fruit decay.					
35700848	7	43	theme	antioxidant	1161:1171	arg1	ability					1173:1179	antioxidant ability	1161:1179	antioxidant ability	1161:1179	In addition, Cur-HKUST-1@CMS/PVA had excellent antibacterial activity and antioxidant ability.					
35700848	1	44	theme	waste	234:238	arg1	causes					218:223	the most important causes	199:223	the most important causes of fruit waste	199:238	The spoilage of fruit is one of the most important causes of fruit waste.					
35700848	0	45	theme	moisture-responsive	68:86	arg1	properties					96:105	moisture-responsive release properties	68:105	moisture-responsive release properties	68:105	Curcumin-loaded HKUST-1@ carboxymethyl starch-based composites with moisture-responsive release properties and synergistic antibacterial effect for perishable fruits.					
35700848	6	46	theme	25.11 	1040:1045	arg1	%					1046:1046	%	1046:1046	%	1046:1046	XRD and SEM demonstrated that moisture disrupts the structure of HKUST-1 and releases curcumin and the results showed that the release of curcumin increased from 25.11 % to 58.32 % after moisture stimulation.					
35700848	5	47	theme	curcumin	748:755	arg1	HKUST-1					723:729	HKUST-1	723:729	HKUST-1	723:729	HKUST-1 not only acts as curcumin carriers but also forms synergistic antibacterial with curcumin to improve the antibacterial activity of the composites.					
35700848	5	47	theme	curcumin	748:755	arg1	antibacterial					793:805	synergistic antibacterial	781:805	synergistic antibacterial with curcumin	781:819	HKUST-1 not only acts as curcumin carriers but also forms synergistic antibacterial with curcumin to improve the antibacterial activity of the composites.					
35700848	5	47	theme	curcumin	748:755	arg1	carriers					757:764	curcumin carriers	748:764	curcumin carriers	748:764	HKUST-1 not only acts as curcumin carriers but also forms synergistic antibacterial with curcumin to improve the antibacterial activity of the composites.					
35700848	2	48	theme	High	241:244	arg1	humidity					246:253	High humidity	241:253	High humidity by fresh fruit respiration	241:280	High humidity by fresh fruit respiration leads to bacterial reproduction, which is the key factor of products corruption.					
35700848	2	49	theme	corruption	351:360	arg1	reproduction					301:312	bacterial reproduction	291:312	bacterial reproduction	291:312	High humidity by fresh fruit respiration leads to bacterial reproduction, which is the key factor of products corruption.					
35700848	2	49	theme	corruption	351:360	arg1	factor					332:337	the key factor	324:337	the key factor of products corruption	324:360	High humidity by fresh fruit respiration leads to bacterial reproduction, which is the key factor of products corruption.					
35700848	3	50	theme	@	417:417	arg1	film					400:403	a biological multifunctional film	371:403	a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment	371:482	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	3	50	theme	@	417:417	arg1	CMS/PVA					418:424	Cur-HKUST-1@CMS/PVA	406:424	Cur-HKUST-1@CMS/PVA	406:424	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	6	51	theme	HKUST-1	943:949	arg1	structure					930:938	the structure	926:938	the structure of HKUST-1	926:949	XRD and SEM demonstrated that moisture disrupts the structure of HKUST-1 and releases curcumin and the results showed that the release of curcumin increased from 25.11 % to 58.32 % after moisture stimulation.					
35700848	2	52	theme	products	342:349	arg1	corruption					351:360	products corruption	342:360	products corruption	342:360	High humidity by fresh fruit respiration leads to bacterial reproduction, which is the key factor of products corruption.					
35700848	3	53	with	preservation	438:449	arg1	environment					472:482	a high moisture environment	456:482	a high moisture environment	456:482	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	7	54	theme	antibacterial	1134:1146	arg1	activity					1148:1155	excellent antibacterial activity	1124:1155	excellent antibacterial activity	1124:1155	In addition, Cur-HKUST-1@CMS/PVA had excellent antibacterial activity and antioxidant ability.					
35700848	4	55	from	HKUST-1	714:720	arg1	release					701:707	curcumin release	692:707	curcumin release from HKUST-1	692:720	The hydrophilic CMS facilitates water adsorption and moisture can stimulate curcumin release from HKUST-1.					
35700848	9	56	theme	Cur-HKUST-1	1378:1388	arg1	CMS/PVA					1390:1396	Cur-HKUST-1@CMS/PVA	1378:1396	Cur-HKUST-1@CMS/PVA	1378:1396	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	9	56	theme	Cur-HKUST-1	1378:1388	arg1	material					1430:1437	a promising active packaging material	1401:1437	a promising active packaging material for improve the shelf life of perishable fruits	1401:1485	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	8	57	dep	4 days	1253:1258	arg1	longer					1260:1265	longer	1260:1265	longer	1260:1265	As validation, the film can keep pitaya and avocado freshness at least 4 days longer than the control, confirming the effectiveness of Cur-HKUST-1@CMS/PVA in preventing fruit decay.					
35700848	3	58	theme	CMS	537:539	arg1	alcohol					552:558	carboxymethyl starch (CMS)/polyvinyl alcohol	515:558	carboxymethyl starch (CMS)/polyvinyl alcohol (PVA)	515:564	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	3	58	theme	CMS	537:539	arg1	PVA					561:563	PVA	561:563	PVA	561:563	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	8	59	theme	@	1328:1328	arg1	CMS/PVA					1329:1335	Cur-HKUST-1@CMS/PVA	1317:1335	Cur-HKUST-1@CMS/PVA	1317:1335	As validation, the film can keep pitaya and avocado freshness at least 4 days longer than the control, confirming the effectiveness of Cur-HKUST-1@CMS/PVA in preventing fruit decay.					
35700848	9	60	theme	@	1389:1389	arg1	CMS/PVA					1390:1396	Cur-HKUST-1@CMS/PVA	1378:1396	Cur-HKUST-1@CMS/PVA	1378:1396	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	9	60	theme	@	1389:1389	arg1	material					1430:1437	a promising active packaging material	1401:1437	a promising active packaging material for improve the shelf life of perishable fruits	1401:1485	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	9	61	theme	perishable	1469:1478	arg1	fruits					1480:1485	perishable fruits	1469:1485	perishable fruits	1469:1485	Consequently, Cur-HKUST-1@CMS/PVA is a promising active packaging material for improve the shelf life of perishable fruits.					
35700848	3	62	theme	high	458:461	arg1	environment					472:482	a high moisture environment	456:482	a high moisture environment	456:482	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	2	63	theme	fruit	264:268	arg1	respiration					270:280	fresh fruit respiration	258:280	fresh fruit respiration	258:280	High humidity by fresh fruit respiration leads to bacterial reproduction, which is the key factor of products corruption.					
35700848	3	64	theme	moisture	463:470	arg1	environment					472:482	a high moisture environment	456:482	a high moisture environment	456:482	Herein, a biological multifunctional film (Cur-HKUST-1@CMS/PVA) for fruits preservation with a high moisture environment was developed by cross-linking carboxymethyl starch (CMS)/polyvinyl alcohol (PVA) with MOF-199 (HKUST-1), and loaded with curcumin.					
35700848	6	65	theme	moisture	1065:1072	arg1	stimulation					1074:1084	moisture stimulation	1065:1084	moisture stimulation	1065:1084	XRD and SEM demonstrated that moisture disrupts the structure of HKUST-1 and releases curcumin and the results showed that the release of curcumin increased from 25.11 % to 58.32 % after moisture stimulation.					
35700848	6	66	theme	curcumin	1016:1023	arg1	release					1005:1011	the release	1001:1011	the release of curcumin	1001:1023	XRD and SEM demonstrated that moisture disrupts the structure of HKUST-1 and releases curcumin and the results showed that the release of curcumin increased from 25.11 % to 58.32 % after moisture stimulation.					
35700848	0	67	theme	release	88:94	arg1	properties					96:105	moisture-responsive release properties	68:105	moisture-responsive release properties	68:105	Curcumin-loaded HKUST-1@ carboxymethyl starch-based composites with moisture-responsive release properties and synergistic antibacterial effect for perishable fruits.					
35700848	2	68	theme	fresh	258:262	arg1	respiration					270:280	fresh fruit respiration	258:280	fresh fruit respiration	258:280	High humidity by fresh fruit respiration leads to bacterial reproduction, which is the key factor of products corruption.					
35700848	0	69	with	composites	52:61	arg1	effect					137:142	synergistic antibacterial effect	111:142	synergistic antibacterial effect	111:142	Curcumin-loaded HKUST-1@ carboxymethyl starch-based composites with moisture-responsive release properties and synergistic antibacterial effect for perishable fruits.					
35700848	0	69	with	composites	52:61	arg1	properties					96:105	moisture-responsive release properties	68:105	moisture-responsive release properties	68:105	Curcumin-loaded HKUST-1@ carboxymethyl starch-based composites with moisture-responsive release properties and synergistic antibacterial effect for perishable fruits.					
35700848	1	70	theme	fruit	183:187	arg1	spoilage					171:178	The spoilage	167:178	The spoilage of fruit	167:187	The spoilage of fruit is one of the most important causes of fruit waste.					
35700848	1	70	theme	fruit	183:187	arg1	causes					218:223	the most important causes	199:223	the most important causes of fruit waste	199:238	The spoilage of fruit is one of the most important causes of fruit waste.					
35700848	1	70	theme	fruit	183:187	arg1	one					192:194	one	192:194	one	192:194	The spoilage of fruit is one of the most important causes of fruit waste.					
36904149	7	0	theme	caspase-3	1227:1235	arg1	levels					1237:1242	cleaved caspase-3 levels	1219:1242	cleaved caspase-3 levels	1219:1242	Moreover, FMT gavage ameliorated LPS/D-gal induced liver apoptosis and markedly reduced cleaved caspase-3 levels, and improved histopathological features of the liver.					
36904149	1	1	theme	necrosis	266:273	arg1	occurrence					233:242	the occurrence	229:242	the occurrence of massive hepatocyte necrosis in a short time	229:289	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	5	2	theme	mouse	782:786	arg1	model					788:792	a mouse model	780:792	a mouse model of lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF	780:856	Here, we established a mouse model of lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF to explore the preventive and therapeutic effects of FMT, and its mechanism of action.					
36904149	3	3	theme	intestinal	543:552	arg1	modulation					565:574	intestinal microbiota modulation	543:574	intestinal microbiota modulation	543:574	There exists a relationship between the human intestinal microbiota and liver, so intestinal microbiota modulation may be a strategy for therapy of hepatic diseases.					
36904149	3	3	theme	intestinal	543:552	arg1	strategy					585:592	a strategy	583:592	a strategy for therapy of hepatic diseases	583:624	There exists a relationship between the human intestinal microbiota and liver, so intestinal microbiota modulation may be a strategy for therapy of hepatic diseases.					
36904149	1	4	theme	liver	199:203	arg1	failure					205:211	Acute liver failure	193:211	Acute liver failure (ALF)	193:217	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	1	4	theme	liver	199:203	arg1	ALF					214:216	ALF	214:216	ALF	214:216	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	9	5	theme	Metabolomics	1655:1666	arg1	analysis					1668:1675	Metabolomics analysis	1655:1675	Metabolomics analysis	1655:1675	Metabolomics analysis revealed that FMT significantly altered LPS/D-gal induced disordered liver metabolites.					
36904149	1	6	from	time	286:289	arg1	occurrence					233:242	the occurrence	229:242	the occurrence of massive hepatocyte necrosis in a short time	229:289	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	8	7	dep	modifying	1369:1377	arg1	improving					1414:1422	improving	1414:1422	improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001)	1414:1557	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	1	8	theme	organ	391:395	arg1	failure					397:403	multiple organ failure	382:403	multiple organ failure	382:403	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	4	9	theme	microbiota	654:663	arg1	FMT					682:684	FMT	682:684	FMT	682:684	In previous studies, fecal microbiota transplantation (FMT) from fit donors has been used to modulate intestinal microbiota widely.					
36904149	4	9	theme	microbiota	654:663	arg1	transplantation					665:679	fecal microbiota transplantation	648:679	fecal microbiota transplantation (FMT) from fit donors	648:701	In previous studies, fecal microbiota transplantation (FMT) from fit donors has been used to modulate intestinal microbiota widely.					
36904149	1	10	from	necrosis	266:273	arg1	time					286:289	a short time	278:289	a short time	278:289	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	3	11	theme	hepatic	609:615	arg1	diseases					617:624	hepatic diseases	609:624	hepatic diseases	609:624	There exists a relationship between the human intestinal microbiota and liver, so intestinal microbiota modulation may be a strategy for therapy of hepatic diseases.					
36904149	8	12	theme	FMT	1299:1301	arg1	gavage					1303:1308	FMT gavage	1299:1308	FMT gavage	1299:1308	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	1	13	from	occurrence	233:242	arg1	time					286:289	a short time	278:289	a short time	278:289	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	11	14	theme	therapeutic	2009:2019	arg1	strategy					2021:2028	a potential preventive and therapeutic strategy	1982:2028	a potential preventive and therapeutic strategy for ALF	1982:2036	Our findings suggest that FMT ameliorate ALF by modulating gut microbiota and liver metabolism, and can used as a potential preventive and therapeutic strategy for ALF.					
36904149	9	15	theme	liver	1746:1750	arg1	metabolites					1752:1762	liver metabolites	1746:1762	liver metabolites	1746:1762	Metabolomics analysis revealed that FMT significantly altered LPS/D-gal induced disordered liver metabolites.					
36904149	4	16	theme	fit	692:694	arg1	donors					696:701	fit donors	692:701	fit donors	692:701	In previous studies, fecal microbiota transplantation (FMT) from fit donors has been used to modulate intestinal microbiota widely.					
36904149	7	17	theme	cleaved	1219:1225	arg1	levels					1237:1242	cleaved caspase-3 levels	1219:1242	cleaved caspase-3 levels	1219:1242	Moreover, FMT gavage ameliorated LPS/D-gal induced liver apoptosis and markedly reduced cleaved caspase-3 levels, and improved histopathological features of the liver.					
36904149	1	18	theme	inflammatory	331:342	arg1	response					344:351	inflammatory response	331:351	inflammatory response	331:351	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	11	19	theme	preventive	1994:2003	arg1	strategy					2021:2028	a potential preventive and therapeutic strategy	1982:2028	a potential preventive and therapeutic strategy for ALF	1982:2036	Our findings suggest that FMT ameliorate ALF by modulating gut microbiota and liver metabolism, and can used as a potential preventive and therapeutic strategy for ALF.					
36904149	5	20	theme	preventive	873:882	arg1	effects					900:906	the preventive and therapeutic effects	869:906	the preventive and therapeutic effects of FMT	869:913	Here, we established a mouse model of lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF to explore the preventive and therapeutic effects of FMT, and its mechanism of action.					
36904149	6	21	theme	aminotransferase	981:996	arg1	activity					998:1005	hepatic aminotransferase activity	973:1005	hepatic aminotransferase activity	973:1005	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	8	22	theme	LPS/D-gal-evoked	1324:1339	arg1	dysbiosis					1356:1364	LPS/D-gal-evoked gut microbiota dysbiosis	1324:1364	LPS/D-gal-evoked gut microbiota dysbiosis	1324:1364	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	11	23	theme	potential	1984:1992	arg1	strategy					2021:2028	a potential preventive and therapeutic strategy	1982:2028	a potential preventive and therapeutic strategy for ALF	1982:2036	Our findings suggest that FMT ameliorate ALF by modulating gut microbiota and liver metabolism, and can used as a potential preventive and therapeutic strategy for ALF.					
36904149	5	24	theme	therapeutic	888:898	arg1	effects					900:906	the preventive and therapeutic effects	869:906	the preventive and therapeutic effects of FMT	869:913	Here, we established a mouse model of lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF to explore the preventive and therapeutic effects of FMT, and its mechanism of action.					
36904149	8	25	theme	microbiota	1345:1354	arg1	dysbiosis					1356:1364	LPS/D-gal-evoked gut microbiota dysbiosis	1324:1364	LPS/D-gal-evoked gut microbiota dysbiosis	1324:1364	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	11	26	used	used	1974:1977	arg2	FMT					1896:1898	FMT	1896:1898	FMT	1896:1898	Our findings suggest that FMT ameliorate ALF by modulating gut microbiota and liver metabolism, and can used as a potential preventive and therapeutic strategy for ALF.					
36904149	7	27	theme	induced	1174:1180	arg1	apoptosis					1188:1196	LPS/D-gal induced liver apoptosis	1164:1196	LPS/D-gal induced liver apoptosis	1164:1196	Moreover, FMT gavage ameliorated LPS/D-gal induced liver apoptosis and markedly reduced cleaved caspase-3 levels, and improved histopathological features of the liver.					
36904149	6	28	theme	hepatic	1055:1061	arg1	cytokines					1080:1088	hepatic pro-inflammatory cytokines	1055:1088	hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05)	1055:1128	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	1	29	theme	short	280:284	arg1	time					286:289	a short time	278:289	a short time	278:289	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	3	30	theme	diseases	617:624	arg1	therapy					598:604	therapy	598:604	therapy of hepatic diseases	598:624	There exists a relationship between the human intestinal microbiota and liver, so intestinal microbiota modulation may be a strategy for therapy of hepatic diseases.					
36904149	8	31	theme	microbial	1391:1399	arg1	composition					1401:1411	the colonic microbial composition	1379:1411	the colonic microbial composition	1379:1411	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	7	32	theme	FMT	1141:1143	arg1	gavage					1145:1150	FMT gavage	1141:1150	FMT gavage	1141:1150	Moreover, FMT gavage ameliorated LPS/D-gal induced liver apoptosis and markedly reduced cleaved caspase-3 levels, and improved histopathological features of the liver.					
36904149	11	33	theme	liver	1948:1952	arg1	metabolism					1954:1963	liver metabolism	1948:1963	liver metabolism	1948:1963	Our findings suggest that FMT ameliorate ALF by modulating gut microbiota and liver metabolism, and can used as a potential preventive and therapeutic strategy for ALF.					
36904149	2	34	theme	effective	420:428	arg1	therapies					430:438	effective therapies	420:438	effective therapies for ALF	420:446	Additionally, effective therapies for ALF are lacking.					
36904149	5	35	theme	induced	846:852	arg1	ALF					854:856	lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF	797:856	lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF	797:856	Here, we established a mouse model of lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF to explore the preventive and therapeutic effects of FMT, and its mechanism of action.					
36904149	1	36	theme	multiple	297:304	arg1	complications					306:318	multiple complications	297:318	multiple complications	297:318	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	1	36	theme	multiple	297:304	arg1	failure					397:403	multiple organ failure	382:403	multiple organ failure	382:403	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	1	36	theme	multiple	297:304	arg1	encephalopathy					362:375	hepatic encephalopathy	354:375	hepatic encephalopathy	354:375	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	1	36	theme	multiple	297:304	arg1	response					344:351	inflammatory response	331:351	inflammatory response	331:351	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	0	37	theme	Microbiome	27:36	arg1	Analysis					11:18	Analysis	11:18	Analysis of Gut Microbiome and Liver Metabolome	11:57	Integrated Analysis of Gut Microbiome and Liver Metabolome to Evaluate the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice.					
36904149	7	38	theme	liver	1292:1296	arg1	features					1276:1283	histopathological features	1258:1283	histopathological features of the liver	1258:1296	Moreover, FMT gavage ameliorated LPS/D-gal induced liver apoptosis and markedly reduced cleaved caspase-3 levels, and improved histopathological features of the liver.					
36904149	3	39	theme	human	501:505	arg1	microbiota					518:527	the human intestinal microbiota	497:527	the human intestinal microbiota	497:527	There exists a relationship between the human intestinal microbiota and liver, so intestinal microbiota modulation may be a strategy for therapy of hepatic diseases.					
36904149	0	40	theme	Liver	42:46	arg1	Metabolome					48:57	Liver Metabolome	42:57	Liver Metabolome	42:57	Integrated Analysis of Gut Microbiome and Liver Metabolome to Evaluate the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice.					
36904149	6	41	theme	p	1120:1120	arg1	mice					1114:1117	LPS/D-gal challenged mice	1093:1117	LPS/D-gal challenged mice (p < 0.05)	1093:1128	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	6	41	theme	p	1120:1120	arg1	<					1122:1122	p < 0.05	1120:1127	p < 0.05	1120:1127	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	7	42	theme	histopathological	1258:1274	arg1	features					1276:1283	histopathological features	1258:1283	histopathological features of the liver	1258:1296	Moreover, FMT gavage ameliorated LPS/D-gal induced liver apoptosis and markedly reduced cleaved caspase-3 levels, and improved histopathological features of the liver.					
36904149	6	43	theme	total	1017:1021	arg1	levels					1033:1038	serum total bilirubin levels	1011:1038	serum total bilirubin levels	1011:1038	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	6	44	from	cytokines	1080:1088	arg1	mice					1114:1117	LPS/D-gal challenged mice	1093:1117	LPS/D-gal challenged mice (p < 0.05)	1093:1128	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	6	44	from	cytokines	1080:1088	arg1	<					1122:1122	p < 0.05	1120:1127	p < 0.05	1120:1127	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	5	45	theme	ALF	854:856	arg1	model					788:792	a mouse model	780:792	a mouse model of lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF	780:856	Here, we established a mouse model of lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF to explore the preventive and therapeutic effects of FMT, and its mechanism of action.					
36904149	3	46	dep	relationship	476:487	arg1	modulation					565:574	intestinal microbiota modulation	543:574	intestinal microbiota modulation	543:574	There exists a relationship between the human intestinal microbiota and liver, so intestinal microbiota modulation may be a strategy for therapy of hepatic diseases.					
36904149	3	46	dep	relationship	476:487	arg1	strategy					585:592	a strategy	583:592	a strategy for therapy of hepatic diseases	583:624	There exists a relationship between the human intestinal microbiota and liver, so intestinal microbiota modulation may be a strategy for therapy of hepatic diseases.					
36904149	6	47	theme	LPS/D-gal	1093:1101	arg1	mice					1114:1117	LPS/D-gal challenged mice	1093:1117	LPS/D-gal challenged mice (p < 0.05)	1093:1128	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	6	47	theme	LPS/D-gal	1093:1101	arg1	<					1122:1122	p < 0.05	1120:1127	p < 0.05	1120:1127	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	8	48	theme	p	1598:1598	arg1	Lactobacillus					1583:1595	Lactobacillus	1583:1595	Lactobacillus (p < 0.05)	1583:1606	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	8	48	theme	p	1598:1598	arg1	<					1600:1600	p < 0.05	1598:1605	p < 0.05	1598:1605	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	4	49	from	donors	696:701	arg1	FMT					682:684	FMT	682:684	FMT	682:684	In previous studies, fecal microbiota transplantation (FMT) from fit donors has been used to modulate intestinal microbiota widely.					
36904149	4	49	from	donors	696:701	arg1	transplantation					665:679	fecal microbiota transplantation	648:679	fecal microbiota transplantation (FMT) from fit donors	648:701	In previous studies, fecal microbiota transplantation (FMT) from fit donors has been used to modulate intestinal microbiota widely.					
36904149	1	50	theme	hepatocyte	255:264	arg1	necrosis					266:273	massive hepatocyte necrosis	247:273	massive hepatocyte necrosis in a short time	247:289	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	3	51	theme	microbiota	554:563	arg1	modulation					565:574	intestinal microbiota modulation	543:574	intestinal microbiota modulation	543:574	There exists a relationship between the human intestinal microbiota and liver, so intestinal microbiota modulation may be a strategy for therapy of hepatic diseases.					
36904149	3	51	theme	microbiota	554:563	arg1	strategy					585:592	a strategy	583:592	a strategy for therapy of hepatic diseases	583:624	There exists a relationship between the human intestinal microbiota and liver, so intestinal microbiota modulation may be a strategy for therapy of hepatic diseases.					
36904149	0	52	from	Injury	177:182	arg1	Mice					187:190	Mice	187:190	Mice	187:190	Integrated Analysis of Gut Microbiome and Liver Metabolome to Evaluate the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice.					
36904149	1	53	theme	Acute	193:197	arg1	failure					205:211	Acute liver failure	193:211	Acute liver failure (ALF)	193:217	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	1	53	theme	Acute	193:197	arg1	ALF					214:216	ALF	214:216	ALF	214:216	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	10	54	theme	strong	1796:1801	arg1	correlations					1803:1814	strong correlations	1796:1814	strong correlations between microbiota composition and liver metabolites	1796:1867	Pearson's correlation revealed strong correlations between microbiota composition and liver metabolites.					
36904149	0	55	theme	Lipopolysaccharide/D-galactosamine-Induced	122:163	arg1	Injury					177:182	Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury	122:182	Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice	122:190	Integrated Analysis of Gut Microbiome and Liver Metabolome to Evaluate the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice.					
36904149	0	56	from	Effects	75:81	arg1	Injury					177:182	Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury	122:182	Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice	122:190	Integrated Analysis of Gut Microbiome and Liver Metabolome to Evaluate the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice.					
36904149	0	57	theme	Liver	171:175	arg1	Injury					177:182	Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury	122:182	Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice	122:190	Integrated Analysis of Gut Microbiome and Liver Metabolome to Evaluate the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice.					
36904149	4	58	theme	fecal	648:652	arg1	FMT					682:684	FMT	682:684	FMT	682:684	In previous studies, fecal microbiota transplantation (FMT) from fit donors has been used to modulate intestinal microbiota widely.					
36904149	4	58	theme	fecal	648:652	arg1	transplantation					665:679	fecal microbiota transplantation	648:679	fecal microbiota transplantation (FMT) from fit donors	648:701	In previous studies, fecal microbiota transplantation (FMT) from fit donors has been used to modulate intestinal microbiota widely.					
36904149	7	59	theme	liver	1182:1186	arg1	apoptosis					1188:1196	LPS/D-gal induced liver apoptosis	1164:1196	LPS/D-gal induced liver apoptosis	1164:1196	Moreover, FMT gavage ameliorated LPS/D-gal induced liver apoptosis and markedly reduced cleaved caspase-3 levels, and improved histopathological features of the liver.					
36904149	5	60	theme	FMT	911:913	arg1	mechanism					924:932	its mechanism	920:932	its mechanism of action	920:942	Here, we established a mouse model of lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF to explore the preventive and therapeutic effects of FMT, and its mechanism of action.					
36904149	5	60	theme	FMT	911:913	arg1	effects					900:906	the preventive and therapeutic effects	869:906	the preventive and therapeutic effects of FMT	869:913	Here, we established a mouse model of lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF to explore the preventive and therapeutic effects of FMT, and its mechanism of action.					
36904149	6	61	theme	serum	1011:1015	arg1	levels					1033:1038	serum total bilirubin levels	1011:1038	serum total bilirubin levels	1011:1038	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	8	62	theme	p	1508:1508	arg1	norank_f_Muribaculaceae					1483:1505	norank_f_Muribaculaceae	1483:1505	norank_f_Muribaculaceae (p < 0.001)	1483:1517	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	8	62	theme	p	1508:1508	arg1	<					1510:1510	p < 0.001	1508:1516	p < 0.001	1508:1516	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	10	63	theme	liver	1851:1855	arg1	metabolites					1857:1867	liver metabolites	1851:1867	liver metabolites	1851:1867	Pearson's correlation revealed strong correlations between microbiota composition and liver metabolites.					
36904149	6	64	theme	hepatic	973:979	arg1	activity					998:1005	hepatic aminotransferase activity	973:1005	hepatic aminotransferase activity	973:1005	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	8	65	theme	<	1473:1473	arg1	p					1471:1471	p < 0.001	1471:1479	p < 0.001	1471:1479	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	8	65	theme	<	1473:1473	arg1	unclassified_o_Bacteroidales					1441:1468	unclassified_o_Bacteroidales	1441:1468	unclassified_o_Bacteroidales (p < 0.001)	1441:1480	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	0	66	theme	Fecal	86:90	arg1	Transplantation					103:117	Fecal Microbiota Transplantation	86:117	Fecal Microbiota Transplantation	86:117	Integrated Analysis of Gut Microbiome and Liver Metabolome to Evaluate the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice.					
36904149	1	67	theme	hepatic	354:360	arg1	encephalopathy					362:375	hepatic encephalopathy	354:375	hepatic encephalopathy	354:375	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	5	68	theme	action	937:942	arg1	mechanism					924:932	its mechanism	920:932	its mechanism of action	920:942	Here, we established a mouse model of lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF to explore the preventive and therapeutic effects of FMT, and its mechanism of action.					
36904149	5	68	theme	action	937:942	arg1	effects					900:906	the preventive and therapeutic effects	869:906	the preventive and therapeutic effects of FMT	869:913	Here, we established a mouse model of lipopolysaccharide (LPS)/D-galactosamine (D-gal) induced ALF to explore the preventive and therapeutic effects of FMT, and its mechanism of action.					
36904149	8	69	theme	gut	1341:1343	arg1	dysbiosis					1356:1364	LPS/D-gal-evoked gut microbiota dysbiosis	1324:1364	LPS/D-gal-evoked gut microbiota dysbiosis	1324:1364	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	7	70	theme	LPS/D-gal	1164:1172	arg1	apoptosis					1188:1196	LPS/D-gal induced liver apoptosis	1164:1196	LPS/D-gal induced liver apoptosis	1164:1196	Moreover, FMT gavage ameliorated LPS/D-gal induced liver apoptosis and markedly reduced cleaved caspase-3 levels, and improved histopathological features of the liver.					
36904149	4	71	used	used	712:715	arg2	FMT					682:684	FMT	682:684	FMT	682:684	In previous studies, fecal microbiota transplantation (FMT) from fit donors has been used to modulate intestinal microbiota widely.					
36904149	4	71	used	used	712:715	arg2	transplantation					665:679	fecal microbiota transplantation	648:679	fecal microbiota transplantation (FMT) from fit donors	648:701	In previous studies, fecal microbiota transplantation (FMT) from fit donors has been used to modulate intestinal microbiota widely.					
36904149	6	72	theme	pro-inflammatory	1063:1078	arg1	cytokines					1080:1088	hepatic pro-inflammatory cytokines	1055:1088	hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05)	1055:1128	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	8	73	theme	p	1548:1548	arg1	Prevotellaceae_UCG-001					1524:1545	Prevotellaceae_UCG-001	1524:1545	Prevotellaceae_UCG-001 (p < 0.001)	1524:1557	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	8	73	theme	p	1548:1548	arg1	<					1550:1550	p < 0.001	1548:1556	p < 0.001	1548:1556	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	0	74	theme	Transplantation	103:117	arg1	Effects					75:81	the Effects	71:81	the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice	71:190	Integrated Analysis of Gut Microbiome and Liver Metabolome to Evaluate the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice.					
36904149	1	75	theme	multiple	382:389	arg1	failure					397:403	multiple organ failure	382:403	multiple organ failure	382:403	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
36904149	4	76	theme	intestinal	729:738	arg1	microbiota					740:749	intestinal microbiota	729:749	intestinal microbiota	729:749	In previous studies, fecal microbiota transplantation (FMT) from fit donors has been used to modulate intestinal microbiota widely.					
36904149	11	77	theme	gut	1929:1931	arg1	microbiota					1933:1942	gut microbiota	1929:1942	gut microbiota	1929:1942	Our findings suggest that FMT ameliorate ALF by modulating gut microbiota and liver metabolism, and can used as a potential preventive and therapeutic strategy for ALF.					
36904149	0	78	theme	Gut	23:25	arg1	Microbiome					27:36	Gut Microbiome	23:36	Gut Microbiome	23:36	Integrated Analysis of Gut Microbiome and Liver Metabolome to Evaluate the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice.					
36904149	8	79	theme	colonic	1383:1389	arg1	composition					1401:1411	the colonic microbial composition	1379:1411	the colonic microbial composition	1379:1411	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	6	80	theme	bilirubin	1023:1031	arg1	levels					1033:1038	serum total bilirubin levels	1011:1038	serum total bilirubin levels	1011:1038	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	0	81	theme	Microbiota	92:101	arg1	Transplantation					103:117	Fecal Microbiota Transplantation	86:117	Fecal Microbiota Transplantation	86:117	Integrated Analysis of Gut Microbiome and Liver Metabolome to Evaluate the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice.					
36904149	0	82	theme	Metabolome	48:57	arg1	Analysis					11:18	Analysis	11:18	Analysis of Gut Microbiome and Liver Metabolome	11:57	Integrated Analysis of Gut Microbiome and Liver Metabolome to Evaluate the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice.					
36904149	0	83	theme	Acute	165:169	arg1	Injury					177:182	Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury	122:182	Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice	122:190	Integrated Analysis of Gut Microbiome and Liver Metabolome to Evaluate the Effects of Fecal Microbiota Transplantation on Lipopolysaccharide/D-galactosamine-Induced Acute Liver Injury in Mice.					
36904149	3	84	theme	intestinal	507:516	arg1	microbiota					518:527	the human intestinal microbiota	497:527	the human intestinal microbiota	497:527	There exists a relationship between the human intestinal microbiota and liver, so intestinal microbiota modulation may be a strategy for therapy of hepatic diseases.					
36904149	8	85	theme	p	1644:1644	arg1	<					1646:1646	p < 0.05	1644:1651	p < 0.05	1644:1651	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	8	85	theme	p	1644:1644	arg1	unclassified_f_Lachnospiraceae					1612:1641	unclassified_f_Lachnospiraceae	1612:1641	unclassified_f_Lachnospiraceae (p < 0.05)	1612:1652	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	10	86	theme	microbiota	1824:1833	arg1	composition					1835:1845	microbiota composition	1824:1845	microbiota composition	1824:1845	Pearson's correlation revealed strong correlations between microbiota composition and liver metabolites.					
36904149	6	87	theme	challenged	1103:1112	arg1	mice					1114:1117	LPS/D-gal challenged mice	1093:1117	LPS/D-gal challenged mice (p < 0.05)	1093:1128	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	6	87	theme	challenged	1103:1112	arg1	<					1122:1122	p < 0.05	1120:1127	p < 0.05	1120:1127	We found that FMT decreased hepatic aminotransferase activity and serum total bilirubin levels, and decreased hepatic pro-inflammatory cytokines in LPS/D-gal challenged mice (p < 0.05).					
36904149	4	88	theme	previous	630:637	arg1	studies					639:645	previous studies	630:645	previous studies	630:645	In previous studies, fecal microbiota transplantation (FMT) from fit donors has been used to modulate intestinal microbiota widely.					
36904149	8	89	theme	unclassified_o_Bacteroidales	1441:1468	arg1	norank_f_Muribaculaceae					1483:1505	norank_f_Muribaculaceae	1483:1505	norank_f_Muribaculaceae (p < 0.001)	1483:1517	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	8	89	theme	unclassified_o_Bacteroidales	1441:1468	arg1	<					1550:1550	p < 0.001	1548:1556	p < 0.001	1548:1556	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	8	89	theme	unclassified_o_Bacteroidales	1441:1468	arg1	<					1510:1510	p < 0.001	1508:1516	p < 0.001	1508:1516	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	8	89	theme	unclassified_o_Bacteroidales	1441:1468	arg1	abundance					1428:1436	the abundance	1424:1436	the abundance of unclassified_o_Bacteroidales (p < 0.001)	1424:1480	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	8	89	theme	unclassified_o_Bacteroidales	1441:1468	arg1	Prevotellaceae_UCG-001					1524:1545	Prevotellaceae_UCG-001	1524:1545	Prevotellaceae_UCG-001 (p < 0.001)	1524:1557	FMT gavage also restored LPS/D-gal-evoked gut microbiota dysbiosis by modifying the colonic microbial composition, improving the abundance of unclassified_o_Bacteroidales (p < 0.001), norank_f_Muribaculaceae (p < 0.001), and Prevotellaceae_UCG-001 (p < 0.001), while reducing that of Lactobacillus (p < 0.05) and unclassified_f_Lachnospiraceae (p < 0.05).					
36904149	1	90	theme	massive	247:253	arg1	necrosis					266:273	massive hepatocyte necrosis	247:273	massive hepatocyte necrosis in a short time	247:289	Acute liver failure (ALF) refers to the occurrence of massive hepatocyte necrosis in a short time, with multiple complications, including inflammatory response, hepatic encephalopathy, and multiple organ failure.					
35790006	7	0	theme	Popping	983:989	arg1	ratio					1032:1036	volume expansion ratio	1015:1036	volume expansion ratio	1015:1036	Popping characteristics such as volume expansion ratio, popping yield, and sensory properties of popped grains were studied.					
35790006	7	0	theme	Popping	983:989	arg1	characteristics					991:1005	Popping characteristics	983:1005	Popping characteristics such as volume expansion ratio	983:1036	Popping characteristics such as volume expansion ratio, popping yield, and sensory properties of popped grains were studied.					
35790006	4	1	theme	pericarp	512:519	arg1	thickness					521:529	pericarp thickness	512:529	pericarp thickness	512:529	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	9	2	theme	true	1411:1414	arg1	density					1416:1422	true density	1411:1422	true density (1.41 g cm-3 )	1411:1437	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	2	theme	true	1411:1414	arg1	1.41 g cm-3					1425:1435	1.41 g cm-3	1425:1435	1.41 g cm-3	1425:1435	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	10	3	theme	popping	1570:1576	arg1	yield					1578:1582	the highest popping yield	1558:1582	the highest popping yield (72.83%)	1558:1591	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	10	3	theme	popping	1570:1576	arg1	%					1590:1590	72.83%	1585:1590	72.83%	1585:1590	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	9	4	theme	suitable	1294:1301	arg1	87.5 g kg-1					1321:1331	87.5 g kg-1	1321:1331	87.5 g kg-1	1321:1331	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	4	theme	suitable	1294:1301	arg1	content					1312:1318	suitable moisture content	1294:1318	suitable moisture content (87.5 g kg-1 )	1294:1333	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	10	5	located	observed	1624:1631	arg2	yield					1578:1582	the highest popping yield	1558:1582	the highest popping yield (72.83%)	1558:1591	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	10	5	located	observed	1624:1631	arg2	%					1590:1590	72.83%	1585:1590	72.83%	1585:1590	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	10	5	located	observed	1624:1631	arg1	variety					1664:1670	the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics	1636:1769	the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics	1636:1769	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	10	5	located	observed	1624:1631	arg2	6.15					1614:1617	6.15	1614:1617	6.15	1614:1617	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	10	5	located	observed	1624:1631	arg2	ratio					1607:1611	expansion ratio	1597:1611	expansion ratio (6.15)	1597:1618	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	11	6	theme	desirable	1913:1921	arg1	characteristics					1931:1945	more desirable popping characteristics	1908:1945	more desirable popping characteristics	1908:1945	Pearl millet variety AIMP-92 901 developed by VNMKV (Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani), Parbhani was found to have more desirable popping characteristics (in terms of all the parameters explained in results).					
35790006	0	7	theme	pearl	76:80	arg1	varieties					89:97	selected pearl millet varieties	67:97	selected pearl millet varieties	67:97	Effect of physicochemical properties on popping characteristics of selected pearl millet varieties.					
35790006	4	8	theme	expansion	590:598	arg1	ratio					600:604	the volume expansion ratio and sensory properties	579:627	ratio	600:604	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	5	9	theme	millet	788:793	arg1	varieties					795:803	five pearl millet varieties	777:803	five pearl millet varieties	777:803	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	9	10	theme	other	1516:1520	arg1	varieties					1522:1530	the other varieties	1512:1530	the other varieties that were studied	1512:1548	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	10	11	theme	best	1742:1745	arg1	characteristics					1755:1769	the best popping characteristics	1738:1769	the best popping characteristics	1738:1769	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	7	12	theme	expansion	1022:1030	arg1	ratio					1032:1036	volume expansion ratio	1015:1036	volume expansion ratio	1015:1036	Popping characteristics such as volume expansion ratio, popping yield, and sensory properties of popped grains were studied.					
35790006	9	13	theme	equivalent	1343:1352	arg1	2.07 mm					1364:1370	2.07 mm	1364:1370	2.07 mm	1364:1370	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	13	theme	equivalent	1343:1352	arg1	diameter					1354:1361	lowest equivalent diameter	1336:1361	lowest equivalent diameter (2.07 mm)	1336:1371	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	4	14	theme	popped	632:637	arg1	millet					645:650	popped pearl millet	632:650	popped pearl millet	632:650	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	4	15	theme	millet	645:650	arg1	ratio					600:604	the volume expansion ratio and sensory properties	579:627	ratio	600:604	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	4	15	theme	millet	645:650	arg1	properties					618:627	the volume expansion ratio and sensory properties	579:627	properties	618:627	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	8	16	theme	amylose	1160:1166	arg1	content					1168:1174	amylose content	1160:1174	amylose content	1160:1174	RESULTS It was observed that pericarp thickness and amylose content were positively correlated with the popping qualities of grains.					
35790006	2	17	used	used	321:324	arg2	Popping					266:272	Popping	266:272	Popping	266:272	Popping, a traditional method of millet processing, is used in making ready-to-eat snacks.					
35790006	2	17	used	used	321:324	arg2	method					289:294	a traditional method	275:294	a traditional method of millet processing	275:315	Popping, a traditional method of millet processing, is used in making ready-to-eat snacks.					
35790006	7	18	theme	grains	1087:1092	arg1	ratio					1032:1036	volume expansion ratio	1015:1036	volume expansion ratio	1015:1036	Popping characteristics such as volume expansion ratio, popping yield, and sensory properties of popped grains were studied.					
35790006	7	18	theme	grains	1087:1092	arg1	characteristics					991:1005	Popping characteristics	983:1005	Popping characteristics such as volume expansion ratio	983:1036	Popping characteristics such as volume expansion ratio, popping yield, and sensory properties of popped grains were studied.					
35790006	7	18	theme	grains	1087:1092	arg1	yield					1047:1051	popping yield	1039:1051	popping yield	1039:1051	Popping characteristics such as volume expansion ratio, popping yield, and sensory properties of popped grains were studied.					
35790006	7	18	theme	grains	1087:1092	arg1	properties					1066:1075	sensory properties	1058:1075	sensory properties	1058:1075	Popping characteristics such as volume expansion ratio, popping yield, and sensory properties of popped grains were studied.					
35790006	11	19	theme	Pearl	1772:1776	arg1	variety					1785:1791	Pearl millet variety AIMP-92 901	1772:1803	Pearl millet variety AIMP-92 901 developed by VNMKV (Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani), Parbhani	1772:1888	Pearl millet variety AIMP-92 901 developed by VNMKV (Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani), Parbhani was found to have more desirable popping characteristics (in terms of all the parameters explained in results).					
35790006	9	20	theme	highest	1374:1380	arg1	0.84 g cm-3					1396:1406	0.84 g cm-3	1396:1406	0.84 g cm-3	1396:1406	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	20	theme	highest	1374:1380	arg1	density					1387:1393	highest bulk density	1374:1393	highest bulk density (0.84 g cm-3 )	1374:1408	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	0	21	theme	millet	82:87	arg1	varieties					89:97	selected pearl millet varieties	67:97	selected pearl millet varieties	67:97	Effect of physicochemical properties on popping characteristics of selected pearl millet varieties.					
35790006	0	22	from	Effect	0:5	arg1	characteristics					48:62	popping characteristics	40:62	popping characteristics of selected pearl millet varieties	40:97	Effect of physicochemical properties on popping characteristics of selected pearl millet varieties.					
35790006	7	23	theme	sensory	1058:1064	arg1	ratio					1032:1036	volume expansion ratio	1015:1036	volume expansion ratio	1015:1036	Popping characteristics such as volume expansion ratio, popping yield, and sensory properties of popped grains were studied.					
35790006	7	23	theme	sensory	1058:1064	arg1	properties					1066:1075	sensory properties	1058:1075	sensory properties	1058:1075	Popping characteristics such as volume expansion ratio, popping yield, and sensory properties of popped grains were studied.					
35790006	12	24	theme	Chemical	2020:2027	arg1	Industry					2029:2036	Chemical Industry	2020:2036	Chemical Industry	2020:2036	© 2022 Society of Chemical Industry.					
35790006	5	25	theme	conventional	667:678	arg1	technique					693:701	A conventional salt-popping technique	665:701	METHODOLOGY A conventional salt-popping technique	653:701	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	6	26	theme	pericarp	922:929	arg1	thickness					931:939	pericarp thickness	922:939	pericarp thickness	922:939	Parameters such as color, diameter, density, amylose content, pericarp thickness, and proximate composition were analyzed.					
35790006	7	27	theme	popped	1080:1085	arg1	grains					1087:1092	popped grains	1080:1092	popped grains	1080:1092	Popping characteristics such as volume expansion ratio, popping yield, and sensory properties of popped grains were studied.					
35790006	8	28	theme	grains	1233:1238	arg1	qualities					1220:1228	the popping qualities	1208:1228	the popping qualities of grains	1208:1238	RESULTS It was observed that pericarp thickness and amylose content were positively correlated with the popping qualities of grains.					
35790006	7	29	theme	popping	1039:1045	arg1	yield					1047:1051	popping yield	1039:1051	popping yield	1039:1051	Popping characteristics such as volume expansion ratio, popping yield, and sensory properties of popped grains were studied.					
35790006	1	30	theme	BACKGROUND	100:109	arg1	nutrigrain					157:166	a nutrigrain	155:166	a nutrigrain	155:166	BACKGROUND Pearl millet, commonly known as 'Bajra', is a nutrigrain, mostly used in pulverized form to make unleavened pancakes, dumplings, porridge, etc., in India.					
35790006	1	30	theme	BACKGROUND	100:109	arg1	millet					117:122	BACKGROUND Pearl millet	100:122	BACKGROUND Pearl millet	100:122	BACKGROUND Pearl millet, commonly known as 'Bajra', is a nutrigrain, mostly used in pulverized form to make unleavened pancakes, dumplings, porridge, etc., in India.					
35790006	0	31	theme	physicochemical	10:24	arg1	properties					26:35	physicochemical properties	10:35	physicochemical properties	10:35	Effect of physicochemical properties on popping characteristics of selected pearl millet varieties.					
35790006	10	32	theme	AIMP	1640:1643	arg1	variety					1664:1670	the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics	1636:1769	the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics	1636:1769	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	7	33	theme	volume	1015:1020	arg1	ratio					1032:1036	volume expansion ratio	1015:1036	volume expansion ratio	1015:1036	Popping characteristics such as volume expansion ratio, popping yield, and sensory properties of popped grains were studied.					
35790006	4	34	theme	parameters	446:455	arg1	effect					432:437	the effect	428:437	the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet	428:650	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	0	35	theme	selected	67:74	arg1	varieties					89:97	selected pearl millet varieties	67:97	selected pearl millet varieties	67:97	Effect of physicochemical properties on popping characteristics of selected pearl millet varieties.					
35790006	4	36	theme	pearl	460:464	arg1	millet					466:471	pearl millet	460:471	pearl millet	460:471	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	10	37	theme	pearl	1651:1655	arg1	variety					1664:1670	the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics	1636:1769	the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics	1636:1769	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	6	38	theme	proximate	946:954	arg1	composition					956:966	proximate composition	946:966	proximate composition	946:966	Parameters such as color, diameter, density, amylose content, pericarp thickness, and proximate composition were analyzed.					
35790006	4	39	theme	amylose	532:538	arg1	content					540:546	amylose content	532:546	amylose content	532:546	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	10	40	theme	Conventional	1694:1705	arg1	popping					1712:1718	Conventional salt popping	1694:1718	Conventional salt popping at 260 °C	1694:1728	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	2	41	theme	millet	299:304	arg1	processing					306:315	millet processing	299:315	millet processing	299:315	Popping, a traditional method of millet processing, is used in making ready-to-eat snacks.					
35790006	9	42	theme	amylose	1475:1481	arg1	content					1483:1489	amylose content	1475:1489	amylose content (19.75 g kg-1 )	1475:1505	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	42	theme	amylose	1475:1481	arg1	19.75 g kg-1					1492:1503	19.75 g kg-1	1492:1503	19.75 g kg-1	1492:1503	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	4	43	theme	chemical	490:497	arg1	composition					499:509	chemical composition	490:509	chemical composition	490:509	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	10	44	theme	260 °C.	1675:1681	arg1	CONCLUSION					1683:1692	260 °C. CONCLUSION	1675:1692	260 °C. CONCLUSION	1675:1692	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	4	45	from	effect	432:437	arg1	ratio					600:604	the volume expansion ratio and sensory properties	579:627	ratio	600:604	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	4	45	from	effect	432:437	arg1	properties					618:627	the volume expansion ratio and sensory properties	579:627	properties	618:627	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	9	46	theme	bulk	1382:1385	arg1	0.84 g cm-3					1396:1406	0.84 g cm-3	1396:1406	0.84 g cm-3	1396:1406	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	46	theme	bulk	1382:1385	arg1	density					1387:1393	highest bulk density	1374:1393	highest bulk density (0.84 g cm-3 )	1374:1408	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	10	47	theme	highest	1562:1568	arg1	yield					1578:1582	the highest popping yield	1558:1582	the highest popping yield (72.83%)	1558:1591	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	10	47	theme	highest	1562:1568	arg1	%					1590:1590	72.83%	1585:1590	72.83%	1585:1590	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	9	48	theme	moisture	1303:1310	arg1	87.5 g kg-1					1321:1331	87.5 g kg-1	1321:1331	87.5 g kg-1	1321:1331	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	48	theme	moisture	1303:1310	arg1	content					1312:1318	suitable moisture content	1294:1318	suitable moisture content (87.5 g kg-1 )	1294:1333	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	5	49	dep	temperatures	731:742	arg1	260 °C					765:770	260 °C	765:770	260 °C	765:770	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	5	49	dep	temperatures	731:742	arg1	220 °C					745:750	220 °C	745:750	220 °C	745:750	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	5	49	dep	temperatures	731:742	arg1	temperatures					731:742	three different temperatures	715:742	three different temperatures (220 °C, 240 °C, and 260 °C)	715:771	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	5	49	dep	temperatures	731:742	arg1	240 °C					753:758	240 °C	753:758	240 °C	753:758	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	5	50	theme	pearl	782:786	arg1	varieties					795:803	five pearl millet varieties	777:803	five pearl millet varieties	777:803	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	11	51	theme	popping	1923:1929	arg1	characteristics					1931:1945	more desirable popping characteristics	1908:1945	more desirable popping characteristics	1908:1945	Pearl millet variety AIMP-92 901 developed by VNMKV (Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani), Parbhani was found to have more desirable popping characteristics (in terms of all the parameters explained in results).					
35790006	4	52	theme	volume	583:588	arg1	ratio					600:604	the volume expansion ratio and sensory properties	579:627	ratio	600:604	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	11	53	dep	have	1903:1906	arg1	terms					1951:1955	terms	1951:1955	terms of all the parameters explained in results	1951:1998	Pearl millet variety AIMP-92 901 developed by VNMKV (Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani), Parbhani was found to have more desirable popping characteristics (in terms of all the parameters explained in results).					
35790006	10	54	theme	popping	1747:1753	arg1	characteristics					1755:1769	the best popping characteristics	1738:1769	the best popping characteristics	1738:1769	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	5	55	dep	used	707:710	arg1	4-3					811:813	ABPC 4-3	806:813	ABPC 4-3	806:813	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	1	56	from	pancakes	219:226	arg1	India					259:263	India	259:263	India	259:263	BACKGROUND Pearl millet, commonly known as 'Bajra', is a nutrigrain, mostly used in pulverized form to make unleavened pancakes, dumplings, porridge, etc., in India.					
35790006	9	57	theme	lowest	1336:1341	arg1	2.07 mm					1364:1370	2.07 mm	1364:1370	2.07 mm	1364:1370	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	57	theme	lowest	1336:1341	arg1	diameter					1354:1361	lowest equivalent diameter	1336:1361	lowest equivalent diameter (2.07 mm)	1336:1371	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	10	58	dep	variety	1664:1670	arg1	yielded					1730:1736	yielded	1730:1736	yielded the best popping characteristics	1730:1769	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	4	59	theme	present	401:407	arg1	work					409:412	The present work	397:412	The present work	397:412	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	4	60	theme	processing	553:562	arg1	temperature					564:574	processing temperature	553:574	processing temperature	553:574	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	10	61	theme	expansion	1597:1605	arg1	ratio					1607:1611	expansion ratio	1597:1611	expansion ratio (6.15)	1597:1618	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	10	61	theme	expansion	1597:1605	arg1	6.15					1614:1617	6.15	1614:1617	6.15	1614:1617	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	10	62	from	260 °C	1723:1728	arg1	popping					1712:1718	Conventional salt popping	1694:1718	Conventional salt popping at 260 °C	1694:1728	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	8	63	theme	pericarp	1137:1144	arg1	thickness					1146:1154	pericarp thickness	1137:1154	pericarp thickness	1137:1154	RESULTS It was observed that pericarp thickness and amylose content were positively correlated with the popping qualities of grains.					
35790006	11	64	theme	millet	1778:1783	arg1	variety					1785:1791	Pearl millet variety AIMP-92 901	1772:1803	Pearl millet variety AIMP-92 901 developed by VNMKV (Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani), Parbhani	1772:1888	Pearl millet variety AIMP-92 901 developed by VNMKV (Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani), Parbhani was found to have more desirable popping characteristics (in terms of all the parameters explained in results).					
35790006	5	65	dep	METHODOLOGY	653:663	arg1	technique					693:701	A conventional salt-popping technique	665:701	METHODOLOGY A conventional salt-popping technique	653:701	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	4	66	theme	pearl	639:643	arg1	millet					645:650	popped pearl millet	632:650	popped pearl millet	632:650	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	5	67	theme	salt-popping	680:691	arg1	technique					693:701	A conventional salt-popping technique	665:701	METHODOLOGY A conventional salt-popping technique	653:701	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	0	68	theme	varieties	89:97	arg1	characteristics					48:62	popping characteristics	40:62	popping characteristics of selected pearl millet varieties	40:97	Effect of physicochemical properties on popping characteristics of selected pearl millet varieties.					
35790006	5	69	theme	ABPC	806:809	arg1	4-3					811:813	ABPC 4-3	806:813	ABPC 4-3	806:813	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	4	70	theme	sensory	610:616	arg1	properties					618:627	the volume expansion ratio and sensory properties	579:627	properties	618:627	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	5	71	used	used	707:710	arg2	METHODOLOGY					653:663	METHODOLOGY	653:663	METHODOLOGY A conventional salt-popping technique	653:701	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	8	72	theme	popping	1212:1218	arg1	qualities					1220:1228	the popping qualities	1208:1228	the popping qualities of grains	1208:1238	RESULTS It was observed that pericarp thickness and amylose content were positively correlated with the popping qualities of grains.					
35790006	8	73	dep	RESULTS	1108:1114	arg1	observed					1123:1130	observed	1123:1130	was observed that pericarp thickness and amylose content were positively correlated with the popping qualities of grains	1119:1238	RESULTS It was observed that pericarp thickness and amylose content were positively correlated with the popping qualities of grains.					
35790006	5	74	dep	4-3	811:813	arg1	AHB					816:818	AHB	816:818	AHB	816:818	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	5	74	dep	4-3	811:813	arg1	AHB					826:828	AHB	826:828	AHB	826:828	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	5	74	dep	4-3	811:813	arg1	AIMP					836:839	AIMP	836:839	AIMP	836:839	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	5	74	dep	4-3	811:813	arg1	PPC-6					852:856	PPC-6	852:856	PPC-6	852:856	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	0	75	theme	properties	26:35	arg1	Effect					0:5	Effect	0:5	Effect of physicochemical properties on popping characteristics of selected pearl millet varieties.	0:98	Effect of physicochemical properties on popping characteristics of selected pearl millet varieties.					
35790006	1	76	theme	Pearl	111:115	arg1	nutrigrain					157:166	a nutrigrain	155:166	a nutrigrain	155:166	BACKGROUND Pearl millet, commonly known as 'Bajra', is a nutrigrain, mostly used in pulverized form to make unleavened pancakes, dumplings, porridge, etc., in India.					
35790006	1	76	theme	Pearl	111:115	arg1	millet					117:122	BACKGROUND Pearl millet	100:122	BACKGROUND Pearl millet	100:122	BACKGROUND Pearl millet, commonly known as 'Bajra', is a nutrigrain, mostly used in pulverized form to make unleavened pancakes, dumplings, porridge, etc., in India.					
35790006	6	77	theme	amylose	905:911	arg1	content					913:919	amylose content	905:919	amylose content	905:919	Parameters such as color, diameter, density, amylose content, pericarp thickness, and proximate composition were analyzed.					
35790006	10	78	theme	92901	1645:1649	arg1	variety					1664:1670	the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics	1636:1769	the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics	1636:1769	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	0	79	theme	popping	40:46	arg1	characteristics					48:62	popping characteristics	40:62	popping characteristics of selected pearl millet varieties	40:97	Effect of physicochemical properties on popping characteristics of selected pearl millet varieties.					
35790006	3	80	theme	Pearl	357:361	arg1	millet					363:368	Pearl millet	357:368	Pearl millet	357:368	Pearl millet is underutilized in India.					
35790006	2	81	theme	ready-to-eat	336:347	arg1	snacks					349:354	ready-to-eat snacks	336:354	ready-to-eat snacks	336:354	Popping, a traditional method of millet processing, is used in making ready-to-eat snacks.					
35790006	5	82	theme	different	721:729	arg1	260 °C					765:770	260 °C	765:770	260 °C	765:770	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	5	82	theme	different	721:729	arg1	220 °C					745:750	220 °C	745:750	220 °C	745:750	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	5	82	theme	different	721:729	arg1	temperatures					731:742	three different temperatures	715:742	three different temperatures (220 °C, 240 °C, and 260 °C)	715:771	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	5	82	theme	different	721:729	arg1	240 °C					753:758	240 °C	753:758	240 °C	753:758	METHODOLOGY A conventional salt-popping technique was used at three different temperatures (220 °C, 240 °C, and 260 °C) for five pearl millet varieties (ABPC 4-3, AHB 1269, AHB 1666, AIMP 92901, and PPC-6).					
35790006	9	83	theme	pericarp	1440:1447	arg1	30.82 μm					1460:1467	30.82 μm	1460:1467	30.82 μm	1460:1467	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	83	theme	pericarp	1440:1447	arg1	thickness					1449:1457	pericarp thickness	1440:1457	pericarp thickness (30.82 μm)	1440:1468	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	11	84	theme	parameters	1968:1977	arg1	terms					1951:1955	terms	1951:1955	terms of all the parameters explained in results	1951:1998	Pearl millet variety AIMP-92 901 developed by VNMKV (Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani), Parbhani was found to have more desirable popping characteristics (in terms of all the parameters explained in results).					
35790006	4	85	theme	millet	466:471	arg1	temperature					564:574	processing temperature	553:574	processing temperature	553:574	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	4	85	theme	millet	466:471	arg1	variety					481:487	variety	481:487	variety	481:487	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	4	85	theme	millet	466:471	arg1	thickness					521:529	pericarp thickness	512:529	pericarp thickness	512:529	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	4	85	theme	millet	466:471	arg1	parameters					446:455	the parameters	442:455	the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature	442:574	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	4	85	theme	millet	466:471	arg1	content					540:546	amylose content	532:546	amylose content	532:546	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	4	85	theme	millet	466:471	arg1	composition					499:509	chemical composition	490:509	chemical composition	490:509	The present work aims to study the effect of the parameters of pearl millet such as variety, chemical composition, pericarp thickness, amylose content, and processing temperature on the volume expansion ratio and sensory properties of popped pearl millet.					
35790006	2	86	theme	traditional	277:287	arg1	method					289:294	a traditional method	275:294	a traditional method of millet processing	275:315	Popping, a traditional method of millet processing, is used in making ready-to-eat snacks.					
35790006	2	86	theme	traditional	277:287	arg1	Popping					266:272	Popping	266:272	Popping	266:272	Popping, a traditional method of millet processing, is used in making ready-to-eat snacks.					
35790006	9	87	theme	AIMP	1241:1244	arg1	92901					1246:1250	AIMP 92901	1241:1250	AIMP 92901	1241:1250	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	10	88	theme	millet	1657:1662	arg1	variety					1664:1670	the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics	1636:1769	the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics	1636:1769	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	1	89	theme	pulverized	184:193	arg1	form					195:198	pulverized form	184:198	pulverized form	184:198	BACKGROUND Pearl millet, commonly known as 'Bajra', is a nutrigrain, mostly used in pulverized form to make unleavened pancakes, dumplings, porridge, etc., in India.					
35790006	11	90	contain	have	1903:1906	arg2	characteristics					1931:1945	more desirable popping characteristics	1908:1945	more desirable popping characteristics	1908:1945	Pearl millet variety AIMP-92 901 developed by VNMKV (Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani), Parbhani was found to have more desirable popping characteristics (in terms of all the parameters explained in results).					
35790006	11	90	contain	have	1903:1906	arg1	variety					1785:1791	Pearl millet variety AIMP-92 901	1772:1803	Pearl millet variety AIMP-92 901 developed by VNMKV (Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani), Parbhani	1772:1888	Pearl millet variety AIMP-92 901 developed by VNMKV (Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani), Parbhani was found to have more desirable popping characteristics (in terms of all the parameters explained in results).					
35790006	10	91	theme	salt	1707:1710	arg1	popping					1712:1718	Conventional salt popping	1694:1718	Conventional salt popping at 260 °C	1694:1728	Hence, the highest popping yield (72.83%) and expansion ratio (6.15) was observed in the AIMP 92901 pearl millet variety at 260 °C. CONCLUSION Conventional salt popping at 260 °C yielded the best popping characteristics.					
35790006	9	92	theme	desirable	1265:1273	arg1	thickness					1449:1457	pericarp thickness	1440:1457	pericarp thickness (30.82 μm)	1440:1468	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	92	theme	desirable	1265:1273	arg1	content					1312:1318	suitable moisture content	1294:1318	suitable moisture content (87.5 g kg-1 )	1294:1333	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	92	theme	desirable	1265:1273	arg1	density					1387:1393	highest bulk density	1374:1393	highest bulk density (0.84 g cm-3 )	1374:1408	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	92	theme	desirable	1265:1273	arg1	content					1483:1489	amylose content	1475:1489	amylose content (19.75 g kg-1 )	1475:1505	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	92	theme	desirable	1265:1273	arg1	density					1416:1422	true density	1411:1422	true density (1.41 g cm-3 )	1411:1437	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	92	theme	desirable	1265:1273	arg1	diameter					1354:1361	lowest equivalent diameter	1336:1361	lowest equivalent diameter (2.07 mm)	1336:1371	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	9	92	theme	desirable	1265:1273	arg1	properties					1275:1284	more desirable properties	1260:1284	more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 )	1260:1505	AIMP 92901 offered more desirable properties such as suitable moisture content (87.5 g kg-1 ), lowest equivalent diameter (2.07 mm), highest bulk density (0.84 g cm-3 ), true density (1.41 g cm-3 ), pericarp thickness (30.82 μm), and amylose content (19.75 g kg-1 ) than the other varieties that were studied.					
35790006	2	93	theme	processing	306:315	arg1	method					289:294	a traditional method	275:294	a traditional method of millet processing	275:315	Popping, a traditional method of millet processing, is used in making ready-to-eat snacks.					
35790006	2	93	theme	processing	306:315	arg1	Popping					266:272	Popping	266:272	Popping	266:272	Popping, a traditional method of millet processing, is used in making ready-to-eat snacks.					
35790006	1	94	theme	unleavened	208:217	arg1	etc.					250:253	etc.	250:253	etc.	250:253	BACKGROUND Pearl millet, commonly known as 'Bajra', is a nutrigrain, mostly used in pulverized form to make unleavened pancakes, dumplings, porridge, etc., in India.					
35790006	1	94	theme	unleavened	208:217	arg1	porridge					240:247	porridge	240:247	porridge	240:247	BACKGROUND Pearl millet, commonly known as 'Bajra', is a nutrigrain, mostly used in pulverized form to make unleavened pancakes, dumplings, porridge, etc., in India.					
35790006	1	94	theme	unleavened	208:217	arg1	dumplings					229:237	dumplings	229:237	dumplings	229:237	BACKGROUND Pearl millet, commonly known as 'Bajra', is a nutrigrain, mostly used in pulverized form to make unleavened pancakes, dumplings, porridge, etc., in India.					
35790006	1	94	theme	unleavened	208:217	arg1	pancakes					219:226	unleavened pancakes	208:226	unleavened pancakes	208:226	BACKGROUND Pearl millet, commonly known as 'Bajra', is a nutrigrain, mostly used in pulverized form to make unleavened pancakes, dumplings, porridge, etc., in India.					
37249403	7	0	theme	%	1295:1295	arg1	swelling					1280:1287	a maximum swelling	1270:1287	a maximum swelling of 432%	1270:1295	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	3	1	theme	hydrogel	691:698	arg1	beads					700:704	ammonium metavanadate (AMV)-biopolymer composite hydrogel beads	642:704	ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters	642:764	In this communication, we report a simple approach to create ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters.					
37249403	0	2	theme	ascorbic	178:185	arg1	sensor					192:197	efficient dye adsorbent and ascorbic acid sensor	150:197	efficient dye adsorbent and ascorbic acid sensor	150:197	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	0	2	theme	ascorbic	178:185	arg1	agent					143:147	an antimicrobial agent	126:147	an antimicrobial agent	126:147	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	7	3	contain	possessed	1193:1201	arg2	behaviour					1254:1262	good swelling behaviour	1240:1262	good swelling behaviour (with a maximum swelling of 432%)	1240:1296	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	7	3	contain	possessed	1193:1201	arg1	blend					1187:1191	the fabricated hydrogel blend	1163:1191	the fabricated hydrogel blend	1163:1191	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	7	3	contain	possessed	1193:1201	arg2	properties					1364:1373	remarkable biomedical properties	1342:1373	remarkable biomedical properties	1342:1373	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	7	3	contain	possessed	1193:1201	arg2	properties					1228:1237	tuneable physicochemical properties	1203:1237	tuneable physicochemical properties	1203:1237	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	7	3	contain	possessed	1193:1201	arg2	luminescence					1309:1320	excellent luminescence	1299:1320	excellent luminescence	1299:1320	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	7	4	theme	fabricated	1167:1176	arg1	blend					1187:1191	the fabricated hydrogel blend	1163:1191	the fabricated hydrogel blend	1163:1191	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	7	5	theme	swelling	1245:1252	arg1	behaviour					1254:1262	good swelling behaviour	1240:1262	good swelling behaviour (with a maximum swelling of 432%)	1240:1296	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	7	6	with	properties	1228:1237	arg1	swelling					1280:1287	a maximum swelling	1270:1287	a maximum swelling of 432%	1270:1295	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	3	7	theme	clusters	757:764	arg1	advantages					723:732	the advantages	719:732	the advantages of biopolymers and POM clusters	719:764	In this communication, we report a simple approach to create ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters.					
37249403	2	8	contain	have	489:492	arg1	class					408:412	a class	406:412	a class of metal-oxygen anionic and nanosized clusters of early transition metals	406:486	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	2	8	contain	have	489:492	arg1	POMs					399:402	POMs	399:402	POMs	399:402	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	2	8	contain	have	489:492	arg2	range					501:505	a wide range	494:505	a wide range of attractive functions	494:529	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	2	8	contain	have	489:492	arg1	polyoxometalates					381:396	inorganic polyoxometalates	371:396	inorganic polyoxometalates (POMs)	371:403	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	7	9	theme	hydrogel	1178:1185	arg1	blend					1187:1191	the fabricated hydrogel blend	1163:1191	the fabricated hydrogel blend	1163:1191	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	2	10	theme	industrial	562:571	arg1	fields					573:578	biomedical and industrial fields	547:578	fields	573:578	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	2	11	theme	biomedical	547:556	arg1	fields					573:578	biomedical and industrial fields	547:578	fields	573:578	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	7	12	with	behaviour	1254:1262	arg1	swelling					1280:1287	a maximum swelling	1270:1287	a maximum swelling of 432%	1270:1295	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	7	13	with	properties	1364:1373	arg1	swelling					1280:1287	a maximum swelling	1270:1287	a maximum swelling of 432%	1270:1295	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	11	14	theme	kidney	2058:2063	arg1	line					2070:2073	embryonic kidney cell line	2048:2073	the embryonic kidney cell line (HEK)	2044:2079	The cytotoxicity results showed that the embedded POMs exhibited dose-dependent cytotoxicity against the embryonic kidney cell line (HEK).					
37249403	11	14	theme	kidney	2058:2063	arg1	HEK					2076:2078	HEK	2076:2078	HEK	2076:2078	The cytotoxicity results showed that the embedded POMs exhibited dose-dependent cytotoxicity against the embryonic kidney cell line (HEK).					
37249403	1	15	theme	widespread	226:235	arg1	attention					237:245	widespread attention	226:245	widespread attention due to their beneficial characteristics, such as like easy processing, biodegradability and biocompatibility	226:354	Biopolymers have received widespread attention due to their beneficial characteristics, such as like easy processing, biodegradability and biocompatibility.					
37249403	8	16	theme	red	1511:1513	arg1	dye					1515:1517	Congo red dye	1505:1517	Congo red dye	1505:1517	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	0	17	theme	efficient	150:158	arg1	sensor					192:197	efficient dye adsorbent and ascorbic acid sensor	150:197	efficient dye adsorbent and ascorbic acid sensor	150:197	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	0	17	theme	efficient	150:158	arg1	agent					143:147	an antimicrobial agent	126:147	an antimicrobial agent	126:147	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	9	18	theme	luminescence	1625:1636	arg1	properties					1638:1647	their luminescence properties	1619:1647	their luminescence properties	1619:1647	Due to their luminescence properties these hydrogel beads showed excellent selective sensing behaviour toward ascorbic acid with a LOD of 1.06 μM.					
37249403	0	19	theme	adsorbent	164:172	arg1	sensor					192:197	efficient dye adsorbent and ascorbic acid sensor	150:197	efficient dye adsorbent and ascorbic acid sensor	150:197	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	0	19	theme	adsorbent	164:172	arg1	agent					143:147	an antimicrobial agent	126:147	an antimicrobial agent	126:147	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	2	20	theme	attractive	510:519	arg1	functions					521:529	attractive functions	510:529	attractive functions	510:529	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	11	21	theme	dose-dependent	2008:2021	arg1	cytotoxicity					2023:2034	dose-dependent cytotoxicity	2008:2034	dose-dependent cytotoxicity against the embryonic kidney cell line (HEK)	2008:2079	The cytotoxicity results showed that the embedded POMs exhibited dose-dependent cytotoxicity against the embryonic kidney cell line (HEK).					
37249403	8	22	theme	Batch	1376:1380	arg1	experiments					1393:1403	Batch adsorption experiments	1376:1403	Batch adsorption experiments	1376:1403	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	0	23	theme	ammonium	65:72	arg1	metavanadate					74:85	ammonium metavanadate	65:85	ammonium metavanadate	65:85	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	3	24	theme	simple	616:621	arg1	approach					623:630	a simple approach	614:630	a simple approach to create ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters	614:764	In this communication, we report a simple approach to create ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters.					
37249403	5	25	theme	hydrogel	932:939	arg1	beads					941:945	The as-prepared hydrogel beads	916:945	The as-prepared hydrogel beads	916:945	The as-prepared hydrogel beads were yellow in colour and exhibited a high mechanical strength.					
37249403	5	25	theme	hydrogel	932:939	arg1	yellow					952:957	yellow	952:957	yellow in colour	952:967	The as-prepared hydrogel beads were yellow in colour and exhibited a high mechanical strength.					
37249403	8	26	contain	had	1433:1435	arg2	capacity					1463:1470	an equilibrium adsorption capacity	1437:1470	an equilibrium adsorption capacity	1437:1470	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	8	26	contain	had	1433:1435	arg1	beads					1427:1431	the beads	1423:1431	the beads	1423:1431	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	8	26	contain	had	1433:1435	arg2	efficient					1558:1566	efficient	1558:1566	efficient	1558:1566	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	6	27	theme	FT-IR	1041:1045	arg1	spectroscopy					1047:1058	FT-IR spectroscopy	1041:1058	FT-IR spectroscopy	1041:1058	They were characterized using FT-IR spectroscopy and SEM, to confirm hydrogel formation and evaluate their surface morphology.					
37249403	8	28	from	solutions	1532:1540	arg1	removal					1494:1500	the removal	1490:1500	the removal of Congo red dye from aqueous solutions	1490:1540	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	0	29	theme	hydrogel	47:54	arg1	beads					56:60	Luminescent and self-healing hybrid ionotropic hydrogel beads	0:60	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.	0:198	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	6	30	theme	surface	1118:1124	arg1	morphology					1126:1135	their surface morphology	1112:1135	their surface morphology	1112:1135	They were characterized using FT-IR spectroscopy and SEM, to confirm hydrogel formation and evaluate their surface morphology.					
37249403	2	31	theme	transition	470:479	arg1	metals					481:486	early transition metals	464:486	early transition metals	464:486	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	8	32	theme	most	1577:1580	arg1	biosorbents					1599:1609	the most reported natural biosorbents	1573:1609	the most reported natural biosorbents	1573:1609	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	0	33	theme	Luminescent	0:10	arg1	beads					56:60	Luminescent and self-healing hybrid ionotropic hydrogel beads	0:60	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.	0:198	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	5	34	from	yellow	952:957	arg1	colour					962:967	colour	962:967	colour	962:967	The as-prepared hydrogel beads were yellow in colour and exhibited a high mechanical strength.					
37249403	8	35	theme	natural	1591:1597	arg1	biosorbents					1599:1609	the most reported natural biosorbents	1573:1609	the most reported natural biosorbents	1573:1609	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	8	36	theme	adsorption	1452:1461	arg1	capacity					1463:1470	an equilibrium adsorption capacity	1437:1470	an equilibrium adsorption capacity	1437:1470	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	8	36	theme	adsorption	1452:1461	arg1	efficient					1558:1566	efficient	1558:1566	efficient	1558:1566	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	2	37	theme	nanosized	442:450	arg1	clusters					452:459	metal-oxygen anionic and nanosized clusters	417:459	metal-oxygen anionic and nanosized clusters of early transition metals	417:486	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	0	38	theme	self-healing	16:27	arg1	beads					56:60	Luminescent and self-healing hybrid ionotropic hydrogel beads	0:60	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.	0:198	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	1	39	theme	easy	301:304	arg1	processing					306:315	easy processing	301:315	easy processing	301:315	Biopolymers have received widespread attention due to their beneficial characteristics, such as like easy processing, biodegradability and biocompatibility.					
37249403	5	40	theme	mechanical	990:999	arg1	strength					1001:1008	a high mechanical strength	983:1008	a high mechanical strength	983:1008	The as-prepared hydrogel beads were yellow in colour and exhibited a high mechanical strength.					
37249403	2	41	theme	anionic	430:436	arg1	clusters					452:459	metal-oxygen anionic and nanosized clusters	417:459	metal-oxygen anionic and nanosized clusters of early transition metals	417:486	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	0	42	theme	ionotropic	36:45	arg1	beads					56:60	Luminescent and self-healing hybrid ionotropic hydrogel beads	0:60	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.	0:198	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	7	43	theme	physicochemical	1212:1226	arg1	properties					1228:1237	tuneable physicochemical properties	1203:1237	tuneable physicochemical properties	1203:1237	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	0	44	dep	beads	56:60	arg1	biomaterial					111:121	biomaterial	111:121	biomaterial	111:121	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	7	45	theme	remarkable	1342:1351	arg1	properties					1364:1373	remarkable biomedical properties	1342:1373	remarkable biomedical properties	1342:1373	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	3	46	theme	ammonium	642:649	arg1	metavanadate					651:662	ammonium metavanadate	642:662	ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters	642:764	In this communication, we report a simple approach to create ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters.					
37249403	3	46	theme	ammonium	642:649	arg1	AMV					665:667	AMV	665:667	AMV	665:667	In this communication, we report a simple approach to create ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters.					
37249403	8	47	theme	mg	1479:1480	arg1	g-1					1482:1484	539 mg g-1	1475:1484	539 mg g-1 for the removal of Congo red dye from aqueous solutions	1475:1540	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	2	48	theme	inorganic	371:379	arg1	class					408:412	a class	406:412	a class of metal-oxygen anionic and nanosized clusters of early transition metals	406:486	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	2	48	theme	inorganic	371:379	arg1	POMs					399:402	POMs	399:402	POMs	399:402	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	2	48	theme	inorganic	371:379	arg1	polyoxometalates					381:396	inorganic polyoxometalates	371:396	inorganic polyoxometalates (POMs)	371:403	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	3	49	theme	composite	681:689	arg1	beads					700:704	ammonium metavanadate (AMV)-biopolymer composite hydrogel beads	642:704	ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters	642:764	In this communication, we report a simple approach to create ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters.					
37249403	9	50	theme	hydrogel	1655:1662	arg1	beads					1664:1668	these hydrogel beads	1649:1668	these hydrogel beads	1649:1668	Due to their luminescence properties these hydrogel beads showed excellent selective sensing behaviour toward ascorbic acid with a LOD of 1.06 μM.					
37249403	9	51	theme	selective	1687:1695	arg1	behaviour					1705:1713	excellent selective sensing behaviour	1677:1713	excellent selective sensing behaviour	1677:1713	Due to their luminescence properties these hydrogel beads showed excellent selective sensing behaviour toward ascorbic acid with a LOD of 1.06 μM.					
37249403	4	52	theme	cationic	836:843	arg1	chitosan					845:852	chitosan	845:852	chitosan	845:852	Crosslinking was achieved through electrostatic interactions between cationic chitosan, chitosan/gelatin, chitosan/methylcellulose and AMV (NH4VO3).					
37249403	11	53	theme	cytotoxicity	1947:1958	arg1	results					1960:1966	The cytotoxicity results	1943:1966	The cytotoxicity results	1943:1966	The cytotoxicity results showed that the embedded POMs exhibited dose-dependent cytotoxicity against the embryonic kidney cell line (HEK).					
37249403	7	54	theme	excellent	1299:1307	arg1	luminescence					1309:1320	excellent luminescence	1299:1320	excellent luminescence	1299:1320	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	7	55	theme	maximum	1272:1278	arg1	swelling					1280:1287	a maximum swelling	1270:1287	a maximum swelling of 432%	1270:1295	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	0	56	theme	acid	187:190	arg1	sensor					192:197	efficient dye adsorbent and ascorbic acid sensor	150:197	efficient dye adsorbent and ascorbic acid sensor	150:197	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	0	56	theme	acid	187:190	arg1	agent					143:147	an antimicrobial agent	126:147	an antimicrobial agent	126:147	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	1	57	theme	beneficial	260:269	arg1	characteristics					271:285	their beneficial characteristics	254:285	their beneficial characteristics	254:285	Biopolymers have received widespread attention due to their beneficial characteristics, such as like easy processing, biodegradability and biocompatibility.					
37249403	1	57	theme	beneficial	260:269	arg1	processing					306:315	easy processing	301:315	easy processing	301:315	Biopolymers have received widespread attention due to their beneficial characteristics, such as like easy processing, biodegradability and biocompatibility.					
37249403	1	57	theme	beneficial	260:269	arg1	biocompatibility					339:354	biocompatibility	339:354	biocompatibility	339:354	Biopolymers have received widespread attention due to their beneficial characteristics, such as like easy processing, biodegradability and biocompatibility.					
37249403	1	57	theme	beneficial	260:269	arg1	biodegradability					318:333	biodegradability	318:333	biodegradability	318:333	Biopolymers have received widespread attention due to their beneficial characteristics, such as like easy processing, biodegradability and biocompatibility.					
37249403	9	58	theme	ascorbic	1722:1729	arg1	acid					1731:1734	ascorbic acid	1722:1734	ascorbic acid	1722:1734	Due to their luminescence properties these hydrogel beads showed excellent selective sensing behaviour toward ascorbic acid with a LOD of 1.06 μM.					
37249403	2	59	used	used	539:542	arg2	POMs					399:402	POMs	399:402	POMs	399:402	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	2	59	used	used	539:542	arg2	polyoxometalates					381:396	inorganic polyoxometalates	371:396	inorganic polyoxometalates (POMs)	371:403	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	2	59	used	used	539:542	arg2	class					408:412	a class	406:412	a class of metal-oxygen anionic and nanosized clusters of early transition metals	406:486	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	3	60	theme	biopolymers	737:747	arg1	advantages					723:732	the advantages	719:732	the advantages of biopolymers and POM clusters	719:764	In this communication, we report a simple approach to create ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters.					
37249403	7	61	with	luminescence	1309:1320	arg1	swelling					1280:1287	a maximum swelling	1270:1287	a maximum swelling of 432%	1270:1295	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	10	62	theme	antibacterial	1802:1814	arg1	activity					1816:1823	their antibacterial activity	1796:1823	their antibacterial activity	1796:1823	The hydrogels were also assessed for their antibacterial activity, and were tested against Staphylococcus aureus, Escherichia coli, Streptococcus anginosus, and Klebsiella pneumoniae.					
37249403	7	63	with	adsorption	1326:1335	arg1	swelling					1280:1287	a maximum swelling	1270:1287	a maximum swelling of 432%	1270:1295	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	0	64	theme	antimicrobial	129:141	arg1	agent					143:147	an antimicrobial agent	126:147	an antimicrobial agent	126:147	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	0	64	theme	antimicrobial	129:141	arg1	sensor					192:197	efficient dye adsorbent and ascorbic acid sensor	150:197	efficient dye adsorbent and ascorbic acid sensor	150:197	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	3	65	theme	POM	753:755	arg1	clusters					757:764	POM clusters	753:764	POM clusters	753:764	In this communication, we report a simple approach to create ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters.					
37249403	11	66	theme	embryonic	2048:2056	arg1	line					2070:2073	embryonic kidney cell line	2048:2073	the embryonic kidney cell line (HEK)	2044:2079	The cytotoxicity results showed that the embedded POMs exhibited dose-dependent cytotoxicity against the embryonic kidney cell line (HEK).					
37249403	11	66	theme	embryonic	2048:2056	arg1	HEK					2076:2078	HEK	2076:2078	HEK	2076:2078	The cytotoxicity results showed that the embedded POMs exhibited dose-dependent cytotoxicity against the embryonic kidney cell line (HEK).					
37249403	8	67	theme	dye	1515:1517	arg1	removal					1494:1500	the removal	1490:1500	the removal of Congo red dye from aqueous solutions	1490:1540	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	0	68	theme	dye	160:162	arg1	sensor					192:197	efficient dye adsorbent and ascorbic acid sensor	150:197	efficient dye adsorbent and ascorbic acid sensor	150:197	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	0	68	theme	dye	160:162	arg1	agent					143:147	an antimicrobial agent	126:147	an antimicrobial agent	126:147	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	9	69	theme	μM	1755:1756	arg1	LOD					1743:1745	a LOD	1741:1745	a LOD of 1.06 μM	1741:1756	Due to their luminescence properties these hydrogel beads showed excellent selective sensing behaviour toward ascorbic acid with a LOD of 1.06 μM.					
37249403	8	70	theme	aqueous	1524:1530	arg1	solutions					1532:1540	aqueous solutions	1524:1540	aqueous solutions	1524:1540	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	0	71	theme	metavanadate	74:85	arg1	beads					56:60	Luminescent and self-healing hybrid ionotropic hydrogel beads	0:60	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.	0:198	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	11	72	theme	cell	2065:2068	arg1	line					2070:2073	embryonic kidney cell line	2048:2073	the embryonic kidney cell line (HEK)	2044:2079	The cytotoxicity results showed that the embedded POMs exhibited dose-dependent cytotoxicity against the embryonic kidney cell line (HEK).					
37249403	11	72	theme	cell	2065:2068	arg1	HEK					2076:2078	HEK	2076:2078	HEK	2076:2078	The cytotoxicity results showed that the embedded POMs exhibited dose-dependent cytotoxicity against the embryonic kidney cell line (HEK).					
37249403	5	73	theme	as-prepared	920:930	arg1	beads					941:945	The as-prepared hydrogel beads	916:945	The as-prepared hydrogel beads	916:945	The as-prepared hydrogel beads were yellow in colour and exhibited a high mechanical strength.					
37249403	5	73	theme	as-prepared	920:930	arg1	yellow					952:957	yellow	952:957	yellow in colour	952:967	The as-prepared hydrogel beads were yellow in colour and exhibited a high mechanical strength.					
37249403	0	74	theme	chitosan	91:98	arg1	beads					56:60	Luminescent and self-healing hybrid ionotropic hydrogel beads	0:60	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.	0:198	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	2	75	theme	wide	496:499	arg1	range					501:505	a wide range	494:505	a wide range of attractive functions	494:529	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	8	76	theme	adsorption	1382:1391	arg1	experiments					1393:1403	Batch adsorption experiments	1376:1403	Batch adsorption experiments	1376:1403	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	4	77	theme	electrostatic	801:813	arg1	interactions					815:826	electrostatic interactions	801:826	electrostatic interactions between cationic chitosan, chitosan/gelatin, chitosan/methylcellulose and AMV (NH4VO3)	801:913	Crosslinking was achieved through electrostatic interactions between cationic chitosan, chitosan/gelatin, chitosan/methylcellulose and AMV (NH4VO3).					
37249403	2	78	theme	functions	521:529	arg1	range					501:505	a wide range	494:505	a wide range of attractive functions	494:529	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	8	79	theme	reported	1582:1589	arg1	biosorbents					1599:1609	the most reported natural biosorbents	1573:1609	the most reported natural biosorbents	1573:1609	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	11	80	theme	embedded	1984:1991	arg1	POMs					1993:1996	the embedded POMs	1980:1996	the embedded POMs	1980:1996	The cytotoxicity results showed that the embedded POMs exhibited dose-dependent cytotoxicity against the embryonic kidney cell line (HEK).					
37249403	7	81	theme	good	1240:1243	arg1	behaviour					1254:1262	good swelling behaviour	1240:1262	good swelling behaviour (with a maximum swelling of 432%)	1240:1296	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	2	82	theme	early	464:468	arg1	metals					481:486	early transition metals	464:486	early transition metals	464:486	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	0	83	theme	hybrid	29:34	arg1	beads					56:60	Luminescent and self-healing hybrid ionotropic hydrogel beads	0:60	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.	0:198	Luminescent and self-healing hybrid ionotropic hydrogel beads of ammonium metavanadate and chitosan: promising biomaterial as an antimicrobial agent, efficient dye adsorbent and ascorbic acid sensor.					
37249403	5	84	theme	high	985:988	arg1	strength					1001:1008	a high mechanical strength	983:1008	a high mechanical strength	983:1008	The as-prepared hydrogel beads were yellow in colour and exhibited a high mechanical strength.					
37249403	7	85	theme	biomedical	1353:1362	arg1	properties					1364:1373	remarkable biomedical properties	1342:1373	remarkable biomedical properties	1342:1373	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	2	86	theme	clusters	452:459	arg1	class					408:412	a class	406:412	a class of metal-oxygen anionic and nanosized clusters of early transition metals	406:486	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	2	86	theme	clusters	452:459	arg1	polyoxometalates					381:396	inorganic polyoxometalates	371:396	inorganic polyoxometalates (POMs)	371:403	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	3	87	theme	metavanadate	651:662	arg1	beads					700:704	ammonium metavanadate (AMV)-biopolymer composite hydrogel beads	642:704	ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters	642:764	In this communication, we report a simple approach to create ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters.					
37249403	8	88	theme	equilibrium	1440:1450	arg1	capacity					1463:1470	an equilibrium adsorption capacity	1437:1470	an equilibrium adsorption capacity	1437:1470	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	8	88	theme	equilibrium	1440:1450	arg1	efficient					1558:1566	efficient	1558:1566	efficient	1558:1566	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	7	89	theme	tuneable	1203:1210	arg1	properties					1228:1237	tuneable physicochemical properties	1203:1237	tuneable physicochemical properties	1203:1237	It was demonstrated that the fabricated hydrogel blend possessed tuneable physicochemical properties, good swelling behaviour (with a maximum swelling of 432%), excellent luminescence and adsorption, and remarkable biomedical properties.					
37249403	6	90	theme	hydrogel	1080:1087	arg1	formation					1089:1097	hydrogel formation	1080:1097	hydrogel formation	1080:1097	They were characterized using FT-IR spectroscopy and SEM, to confirm hydrogel formation and evaluate their surface morphology.					
37249403	2	91	theme	metal-oxygen	417:428	arg1	clusters					452:459	metal-oxygen anionic and nanosized clusters	417:459	metal-oxygen anionic and nanosized clusters of early transition metals	417:486	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	2	92	theme	metals	481:486	arg1	clusters					452:459	metal-oxygen anionic and nanosized clusters	417:459	metal-oxygen anionic and nanosized clusters of early transition metals	417:486	Concurrently, inorganic polyoxometalates (POMs), a class of metal-oxygen anionic and nanosized clusters of early transition metals, have a wide range of attractive functions and are used in biomedical and industrial fields.					
37249403	3	93	theme	-biopolymer	669:679	arg1	beads					700:704	ammonium metavanadate (AMV)-biopolymer composite hydrogel beads	642:704	ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters	642:764	In this communication, we report a simple approach to create ammonium metavanadate (AMV)-biopolymer composite hydrogel beads that combine the advantages of biopolymers and POM clusters.					
37249403	8	94	theme	539	1475:1477	arg1	mg					1479:1480	mg	1479:1480	mg	1479:1480	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	9	95	theme	excellent	1677:1685	arg1	behaviour					1705:1713	excellent selective sensing behaviour	1677:1713	excellent selective sensing behaviour	1677:1713	Due to their luminescence properties these hydrogel beads showed excellent selective sensing behaviour toward ascorbic acid with a LOD of 1.06 μM.					
37249403	8	96	theme	g-1	1482:1484	arg1	capacity					1463:1470	an equilibrium adsorption capacity	1437:1470	an equilibrium adsorption capacity	1437:1470	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	8	96	theme	g-1	1482:1484	arg1	efficient					1558:1566	efficient	1558:1566	efficient	1558:1566	Batch adsorption experiments demonstrated that the beads had an equilibrium adsorption capacity of 539 mg g-1 for the removal of Congo red dye from aqueous solutions, which was more efficient than the most reported natural biosorbents.					
37249403	1	97	theme	due	247:249	arg1	attention					237:245	widespread attention	226:245	widespread attention due to their beneficial characteristics, such as like easy processing, biodegradability and biocompatibility	226:354	Biopolymers have received widespread attention due to their beneficial characteristics, such as like easy processing, biodegradability and biocompatibility.					
37249403	9	98	theme	sensing	1697:1703	arg1	behaviour					1705:1713	excellent selective sensing behaviour	1677:1713	excellent selective sensing behaviour	1677:1713	Due to their luminescence properties these hydrogel beads showed excellent selective sensing behaviour toward ascorbic acid with a LOD of 1.06 μM.					
36079803	4	0	theme	bypass	801:806	arg1	surgery					815:821	Roux-en-Y gastric bypass (RYGB) surgery	783:821	Roux-en-Y gastric bypass (RYGB) surgery	783:821	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	4	1	theme	Roux-en-Y	783:791	arg1	surgery					815:821	Roux-en-Y gastric bypass (RYGB) surgery	783:821	Roux-en-Y gastric bypass (RYGB) surgery	783:821	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	10	2	theme	lipid	1867:1871	arg1	homeostasis					1873:1883	a healthy lipid homeostasis	1857:1883	a healthy lipid homeostasis	1857:1883	Our results suggest that SCFA-producing bacteria promote a healthy lipid homeostasis, while the presence of LPS-producing bacteria such Escherichia-Shigella may contribute to the development of atherogenic dyslipidemia.					
36079803	7	3	theme	LDA	1036:1038	arg1	score					1040:1044	LDA score &gt	1036:1048	LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella)	1036:1105	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	3	4	theme	composition	494:504	arg1	effect					469:474	the effect	465:474	the effect of gut microbiota composition on lipid profiles	465:522	Moreover, because obesity is among the main causes of secondary AD, it is important to analyze the effect of gut microbiota composition on lipid profiles after a weight loss intervention.					
36079803	9	5	theme	PUFA	1592:1595	arg1	intake					1597:1602	PUFA intake	1592:1602	PUFA intake	1592:1602	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	7	6	theme	genera	1024:1029	arg1	abundance					1007:1015	relative abundance	998:1015	relative abundance	998:1015	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	3	7	theme	gut	479:481	arg1	composition					494:504	gut microbiota composition	479:504	gut microbiota composition	479:504	Moreover, because obesity is among the main causes of secondary AD, it is important to analyze the effect of gut microbiota composition on lipid profiles after a weight loss intervention.					
36079803	4	8	theme	weight	760:765	arg1	loss					767:770	weight loss	760:770	weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery	760:821	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	4	9	with	correlations	682:693	arg1	levels					732:737	serum lipid levels	720:737	serum lipid levels in 20 patients	720:752	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	7	10	theme	eligens	1306:1312	arg1	group					1314:1318	Akkermansia and [Eubacterium] eligens group	1276:1318	group	1314:1318	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	7	11	theme	relative	998:1005	arg1	abundance					1007:1015	relative abundance	998:1015	relative abundance	998:1015	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	3	12	theme	lipid	509:513	arg1	profiles					515:522	lipid profiles	509:522	lipid profiles	509:522	Moreover, because obesity is among the main causes of secondary AD, it is important to analyze the effect of gut microbiota composition on lipid profiles after a weight loss intervention.					
36079803	0	13	from	Impact	69:74	arg1	Levels					91:96	Serum Lipid Levels	79:96	Serum Lipid Levels	79:96	Association of Gut Microbiota with Atherogenic Dyslipidemia, and Its Impact on Serum Lipid Levels after Bariatric Surgery.					
36079803	7	14	theme	[Eubacterium	1292:1303	arg1	group					1314:1318	Akkermansia and [Eubacterium] eligens group	1276:1318	group	1314:1318	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	4	15	theme	taxonomic	598:606	arg1	composition					608:618	taxonomic composition	598:618	taxonomic composition	598:618	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	10	16	theme	bacteria	1922:1929	arg1	presence					1896:1903	the presence	1892:1903	the presence of LPS-producing bacteria such Escherichia-Shigella	1892:1955	Our results suggest that SCFA-producing bacteria promote a healthy lipid homeostasis, while the presence of LPS-producing bacteria such Escherichia-Shigella may contribute to the development of atherogenic dyslipidemia.					
36079803	9	17	theme	AD	1636:1637	arg1	subjects					1639:1646	AD subjects	1636:1646	AD subjects	1636:1646	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	7	18	theme	Akkermansia	1276:1286	arg1	group					1314:1318	Akkermansia and [Eubacterium] eligens group	1276:1318	group	1314:1318	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	3	19	theme	weight	532:537	arg1	loss					539:542	a weight loss	530:542	a weight loss intervention	530:555	Moreover, because obesity is among the main causes of secondary AD, it is important to analyze the effect of gut microbiota composition on lipid profiles after a weight loss intervention.					
36079803	2	20	theme	atherogenic	246:256	arg1	AD					272:273	AD	272:273	AD	272:273	However, the relationship between the gut microbiota and atherogenic dyslipidemia (AD), defined as the presence of both low HDL-C and hypertriglyceridemia, is not fully understood.					
36079803	2	20	theme	atherogenic	246:256	arg1	dyslipidemia					258:269	atherogenic dyslipidemia	246:269	atherogenic dyslipidemia (AD)	246:274	However, the relationship between the gut microbiota and atherogenic dyslipidemia (AD), defined as the presence of both low HDL-C and hypertriglyceridemia, is not fully understood.					
36079803	5	21	theme	Gut	824:826	arg1	composition					839:849	Gut microbiota composition	824:849	Gut microbiota composition	824:849	Gut microbiota composition was profiled using next-generation sequencing of 16S rRNA.					
36079803	8	22	theme	group	1390:1394	arg1	abundance					1396:1404	[Eubacterium] eligens group abundance	1368:1404	[Eubacterium] eligens group abundance	1368:1404	Notably, [Eubacterium] eligens group abundance was also significantly associated with higher HDL-C levels in RYGB patients one year after surgery.					
36079803	6	23	theme	microbiota	914:923	arg1	diversity					925:933	Gut microbiota diversity	910:933	Gut microbiota diversity	910:933	Gut microbiota diversity was significantly lower in atherogenic dyslipidemia.					
36079803	4	24	theme	=	643:643	arg1	n					641:641	n = 41	641:646	n = 41	641:646	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	4	24	theme	=	643:643	arg1	patients					623:630	patients	623:630	patients with AD (n = 41)	623:647	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	7	25	theme	AD	1136:1137	arg1	subjects					1139:1146	AD subjects	1136:1146	AD subjects	1136:1146	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	0	26	from	Association	0:10	arg1	Levels					91:96	Serum Lipid Levels	79:96	Serum Lipid Levels	79:96	Association of Gut Microbiota with Atherogenic Dyslipidemia, and Its Impact on Serum Lipid Levels after Bariatric Surgery.					
36079803	0	27	theme	Serum	79:83	arg1	Levels					91:96	Serum Lipid Levels	79:96	Serum Lipid Levels	79:96	Association of Gut Microbiota with Atherogenic Dyslipidemia, and Its Impact on Serum Lipid Levels after Bariatric Surgery.					
36079803	5	28	theme	next-generation	870:884	arg1	sequencing					886:895	next-generation sequencing	870:895	next-generation sequencing of 16S rRNA	870:907	Gut microbiota composition was profiled using next-generation sequencing of 16S rRNA.					
36079803	1	29	theme	Gut	123:125	arg1	microbiota					127:136	Gut microbiota	123:136	Gut microbiota	123:136	Gut microbiota has been suggested to modulate circulating lipids.					
36079803	0	30	theme	Atherogenic	35:45	arg1	Dyslipidemia					47:58	Atherogenic Dyslipidemia	35:58	Atherogenic Dyslipidemia	35:58	Association of Gut Microbiota with Atherogenic Dyslipidemia, and Its Impact on Serum Lipid Levels after Bariatric Surgery.					
36079803	8	31	theme	HDL-C	1452:1456	arg1	levels					1458:1463	higher HDL-C levels	1445:1463	higher HDL-C levels in RYGB patients one year after surgery	1445:1503	Notably, [Eubacterium] eligens group abundance was also significantly associated with higher HDL-C levels in RYGB patients one year after surgery.					
36079803	9	32	theme	Akkermansia	1711:1721	arg1	abundance					1723:1731	only Akkermansia abundance	1706:1731	only Akkermansia abundance	1706:1731	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	3	33	theme	secondary	424:432	arg1	AD					434:435	secondary AD	424:435	secondary AD	424:435	Moreover, because obesity is among the main causes of secondary AD, it is important to analyze the effect of gut microbiota composition on lipid profiles after a weight loss intervention.					
36079803	0	34	theme	Gut	15:17	arg1	Microbiota					19:28	Gut Microbiota	15:28	Gut Microbiota	15:28	Association of Gut Microbiota with Atherogenic Dyslipidemia, and Its Impact on Serum Lipid Levels after Bariatric Surgery.					
36079803	6	35	theme	atherogenic	962:972	arg1	dyslipidemia					974:985	atherogenic dyslipidemia	962:985	atherogenic dyslipidemia	962:985	Gut microbiota diversity was significantly lower in atherogenic dyslipidemia.					
36079803	4	36	with	patients	623:630	arg1	AD					637:638	AD	637:638	AD	637:638	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	4	37	theme	lipid	726:730	arg1	levels					732:737	serum lipid levels	720:737	serum lipid levels in 20 patients	720:752	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	9	38	theme	dietary	1515:1521	arg1	PUFA/SFA					1572:1579	PUFA/SFA	1572:1579	PUFA/SFA	1572:1579	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	9	38	theme	dietary	1515:1521	arg1	acid					1566:1569	dietary polyunsaturated fatty acid/saturated fatty acid	1515:1569	dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio	1515:1586	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	9	39	theme	positive	1742:1749	arg1	correlation					1767:1777	a positive and significant correlation	1740:1777	a positive and significant correlation with PUFA/SFA ratio	1740:1797	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	2	40	theme	HDL-C	313:317	arg1	presence					292:299	the presence	288:299	the presence of both low HDL-C and hypertriglyceridemia	288:342	However, the relationship between the gut microbiota and atherogenic dyslipidemia (AD), defined as the presence of both low HDL-C and hypertriglyceridemia, is not fully understood.					
36079803	9	41	theme	fatty	1539:1543	arg1	PUFA/SFA					1572:1579	PUFA/SFA	1572:1579	PUFA/SFA	1572:1579	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	9	41	theme	fatty	1539:1543	arg1	acid					1566:1569	dietary polyunsaturated fatty acid/saturated fatty acid	1515:1569	dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio	1515:1586	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	7	42	theme	fatty	1189:1193	arg1	SCFA					1202:1205	SCFA	1202:1205	SCFA	1202:1205	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	7	42	theme	fatty	1189:1193	arg1	acids					1195:1199	four short chain fatty acids	1172:1199	four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group)	1172:1319	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	9	43	theme	significant	1755:1765	arg1	correlation					1767:1777	a positive and significant correlation	1740:1777	a positive and significant correlation with PUFA/SFA ratio	1740:1797	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	4	44	theme	genera	698:703	arg1	abundance					705:713	genera abundance	698:713	genera abundance	698:713	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	0	45	with	Association	0:10	arg1	Dyslipidemia					47:58	Atherogenic Dyslipidemia	35:58	Atherogenic Dyslipidemia	35:58	Association of Gut Microbiota with Atherogenic Dyslipidemia, and Its Impact on Serum Lipid Levels after Bariatric Surgery.					
36079803	5	46	theme	rRNA	904:907	arg1	sequencing					886:895	next-generation sequencing	870:895	next-generation sequencing of 16S rRNA	870:907	Gut microbiota composition was profiled using next-generation sequencing of 16S rRNA.					
36079803	7	47	with	genera	1024:1029	arg1	score					1040:1044	LDA score &gt	1036:1048	LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella)	1036:1105	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	10	48	theme	dyslipidemia	2006:2017	arg1	development					1979:1989	the development	1975:1989	the development of atherogenic dyslipidemia	1975:2017	Our results suggest that SCFA-producing bacteria promote a healthy lipid homeostasis, while the presence of LPS-producing bacteria such Escherichia-Shigella may contribute to the development of atherogenic dyslipidemia.					
36079803	10	49	theme	SCFA-producing	1825:1838	arg1	bacteria					1840:1847	SCFA-producing bacteria	1825:1847	SCFA-producing bacteria	1825:1847	Our results suggest that SCFA-producing bacteria promote a healthy lipid homeostasis, while the presence of LPS-producing bacteria such Escherichia-Shigella may contribute to the development of atherogenic dyslipidemia.					
36079803	2	50	theme	hypertriglyceridemia	323:342	arg1	presence					292:299	the presence	288:299	the presence of both low HDL-C and hypertriglyceridemia	288:342	However, the relationship between the gut microbiota and atherogenic dyslipidemia (AD), defined as the presence of both low HDL-C and hypertriglyceridemia, is not fully understood.					
36079803	0	51	with	Impact	69:74	arg1	Dyslipidemia					47:58	Atherogenic Dyslipidemia	35:58	Atherogenic Dyslipidemia	35:58	Association of Gut Microbiota with Atherogenic Dyslipidemia, and Its Impact on Serum Lipid Levels after Bariatric Surgery.					
36079803	3	52	theme	microbiota	483:492	arg1	composition					494:504	gut microbiota composition	479:504	gut microbiota composition	479:504	Moreover, because obesity is among the main causes of secondary AD, it is important to analyze the effect of gut microbiota composition on lipid profiles after a weight loss intervention.					
36079803	10	53	theme	healthy	1859:1865	arg1	homeostasis					1873:1883	a healthy lipid homeostasis	1857:1883	a healthy lipid homeostasis	1857:1883	Our results suggest that SCFA-producing bacteria promote a healthy lipid homeostasis, while the presence of LPS-producing bacteria such Escherichia-Shigella may contribute to the development of atherogenic dyslipidemia.					
36079803	3	54	from	effect	469:474	arg1	profiles					515:522	lipid profiles	509:522	lipid profiles	509:522	Moreover, because obesity is among the main causes of secondary AD, it is important to analyze the effect of gut microbiota composition on lipid profiles after a weight loss intervention.					
36079803	9	55	with	correlation	1767:1777	arg1	ratio					1793:1797	PUFA/SFA ratio	1784:1797	PUFA/SFA ratio	1784:1797	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	1	56	theme	circulating	169:179	arg1	lipids					181:186	circulating lipids	169:186	circulating lipids	169:186	Gut microbiota has been suggested to modulate circulating lipids.					
36079803	7	57	dep	score	1040:1044	arg1	3.5					1050:1052	3.5	1050:1052	3.5	1050:1052	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	4	58	theme	microbial	574:582	arg1	diversity					584:592	the microbial diversity	570:592	the microbial diversity	570:592	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	3	59	theme	loss	539:542	arg1	intervention					544:555	a weight loss intervention	530:555	a weight loss intervention	530:555	Moreover, because obesity is among the main causes of secondary AD, it is important to analyze the effect of gut microbiota composition on lipid profiles after a weight loss intervention.					
36079803	8	60	theme	[Eubacterium	1368:1379	arg1	abundance					1396:1404	[Eubacterium] eligens group abundance	1368:1404	[Eubacterium] eligens group abundance	1368:1404	Notably, [Eubacterium] eligens group abundance was also significantly associated with higher HDL-C levels in RYGB patients one year after surgery.					
36079803	10	61	theme	LPS-producing	1908:1920	arg1	bacteria					1922:1929	LPS-producing bacteria	1908:1929	LPS-producing bacteria	1908:1929	Our results suggest that SCFA-producing bacteria promote a healthy lipid homeostasis, while the presence of LPS-producing bacteria such Escherichia-Shigella may contribute to the development of atherogenic dyslipidemia.					
36079803	8	62	dep	year	1486:1489	arg1	surgery					1497:1503	surgery	1497:1503	surgery	1497:1503	Notably, [Eubacterium] eligens group abundance was also significantly associated with higher HDL-C levels in RYGB patients one year after surgery.					
36079803	4	63	from	levels	732:737	arg1	patients					745:752	20 patients	742:752	20 patients	742:752	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	4	64	theme	gastric	793:799	arg1	surgery					815:821	Roux-en-Y gastric bypass (RYGB) surgery	783:821	Roux-en-Y gastric bypass (RYGB) surgery	783:821	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	8	65	theme	eligens	1382:1388	arg1	abundance					1396:1404	[Eubacterium] eligens group abundance	1368:1404	[Eubacterium] eligens group abundance	1368:1404	Notably, [Eubacterium] eligens group abundance was also significantly associated with higher HDL-C levels in RYGB patients one year after surgery.					
36079803	9	66	theme	acid/saturated	1545:1558	arg1	PUFA/SFA					1572:1579	PUFA/SFA	1572:1579	PUFA/SFA	1572:1579	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	9	66	theme	acid/saturated	1545:1558	arg1	acid					1566:1569	dietary polyunsaturated fatty acid/saturated fatty acid	1515:1569	dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio	1515:1586	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	6	67	theme	Gut	910:912	arg1	diversity					925:933	Gut microbiota diversity	910:933	Gut microbiota diversity	910:933	Gut microbiota diversity was significantly lower in atherogenic dyslipidemia.					
36079803	0	68	theme	Bariatric	104:112	arg1	Surgery					114:120	Bariatric Surgery	104:120	Bariatric Surgery	104:120	Association of Gut Microbiota with Atherogenic Dyslipidemia, and Its Impact on Serum Lipid Levels after Bariatric Surgery.					
36079803	0	69	theme	Lipid	85:89	arg1	Levels					91:96	Serum Lipid Levels	79:96	Serum Lipid Levels	79:96	Association of Gut Microbiota with Atherogenic Dyslipidemia, and Its Impact on Serum Lipid Levels after Bariatric Surgery.					
36079803	9	70	theme	fatty	1560:1564	arg1	PUFA/SFA					1572:1579	PUFA/SFA	1572:1579	PUFA/SFA	1572:1579	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	9	70	theme	fatty	1560:1564	arg1	acid					1566:1569	dietary polyunsaturated fatty acid/saturated fatty acid	1515:1569	dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio	1515:1586	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	8	71	theme	higher	1445:1450	arg1	levels					1458:1463	higher HDL-C levels	1445:1463	higher HDL-C levels in RYGB patients one year after surgery	1445:1503	Notably, [Eubacterium] eligens group abundance was also significantly associated with higher HDL-C levels in RYGB patients one year after surgery.					
36079803	7	72	theme	short	1177:1181	arg1	SCFA					1202:1205	SCFA	1202:1205	SCFA	1202:1205	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	7	72	theme	short	1177:1181	arg1	acids					1195:1199	four short chain fatty acids	1172:1199	four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group)	1172:1319	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	7	73	dep	3.5	1050:1052	arg1	Megasphaera					1055:1065	Megasphaera	1055:1065	Megasphaera	1055:1065	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	7	73	dep	3.5	1050:1052	arg1	Escherichia-Shigella					1085:1104	Escherichia-Shigella	1085:1104	Escherichia-Shigella	1085:1104	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	3	74	theme	main	409:412	arg1	causes					414:419	the main causes	405:419	the main causes of secondary AD	405:435	Moreover, because obesity is among the main causes of secondary AD, it is important to analyze the effect of gut microbiota composition on lipid profiles after a weight loss intervention.					
36079803	5	75	theme	microbiota	828:837	arg1	composition					839:849	Gut microbiota composition	824:849	Gut microbiota composition	824:849	Gut microbiota composition was profiled using next-generation sequencing of 16S rRNA.					
36079803	7	76	theme	producing-genera	1208:1223	arg1	abundance					1159:1167	the abundance	1155:1167	the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group)	1155:1319	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	7	76	theme	producing-genera	1208:1223	arg1	higher					1339:1344	higher	1339:1344	higher	1339:1344	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	8	77	theme	RYGB	1468:1471	arg1	patients					1473:1480	RYGB patients	1468:1480	RYGB patients one year after surgery	1468:1503	Notably, [Eubacterium] eligens group abundance was also significantly associated with higher HDL-C levels in RYGB patients one year after surgery.					
36079803	0	78	theme	Microbiota	19:28	arg1	Impact					69:74	Its Impact	65:74	Its Impact on Serum Lipid Levels after Bariatric Surgery	65:120	Association of Gut Microbiota with Atherogenic Dyslipidemia, and Its Impact on Serum Lipid Levels after Bariatric Surgery.					
36079803	0	78	theme	Microbiota	19:28	arg1	Association					0:10	Association	0:10	Association of Gut Microbiota with Atherogenic Dyslipidemia	0:58	Association of Gut Microbiota with Atherogenic Dyslipidemia, and Its Impact on Serum Lipid Levels after Bariatric Surgery.					
36079803	2	79	theme	gut	227:229	arg1	microbiota					231:240	the gut microbiota	223:240	the gut microbiota	223:240	However, the relationship between the gut microbiota and atherogenic dyslipidemia (AD), defined as the presence of both low HDL-C and hypertriglyceridemia, is not fully understood.					
36079803	4	80	theme	=	665:665	arg1	n					663:663	n = 38	663:668	n = 38	663:668	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	4	80	theme	=	665:665	arg1	controls					653:660	controls	653:660	controls (n = 38)	653:669	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	3	81	theme	AD	434:435	arg1	causes					414:419	the main causes	405:419	the main causes of secondary AD	405:435	Moreover, because obesity is among the main causes of secondary AD, it is important to analyze the effect of gut microbiota composition on lipid profiles after a weight loss intervention.					
36079803	8	82	from	levels	1458:1463	arg1	patients					1473:1480	RYGB patients	1468:1480	RYGB patients one year after surgery	1468:1503	Notably, [Eubacterium] eligens group abundance was also significantly associated with higher HDL-C levels in RYGB patients one year after surgery.					
36079803	10	83	theme	atherogenic	1994:2004	arg1	dyslipidemia					2006:2017	atherogenic dyslipidemia	1994:2017	atherogenic dyslipidemia	1994:2017	Our results suggest that SCFA-producing bacteria promote a healthy lipid homeostasis, while the presence of LPS-producing bacteria such Escherichia-Shigella may contribute to the development of atherogenic dyslipidemia.					
36079803	7	84	theme	acids	1195:1199	arg1	Christensenellaceae					1226:1244	Christensenellaceae	1226:1244	Christensenellaceae	1226:1244	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	7	84	theme	acids	1195:1199	arg1	producing-genera					1208:1223	four short chain fatty acids (SCFA) producing-genera	1172:1223	four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group)	1172:1319	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	4	85	theme	serum	720:724	arg1	levels					732:737	serum lipid levels	720:737	serum lipid levels in 20 patients	720:752	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	7	86	dep	Christensenellaceae	1226:1244	arg1	group					1314:1318	Akkermansia and [Eubacterium] eligens group	1276:1318	group	1314:1318	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	7	87	theme	chain	1183:1187	arg1	SCFA					1202:1205	SCFA	1202:1205	SCFA	1202:1205	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	7	87	theme	chain	1183:1187	arg1	acids					1195:1199	four short chain fatty acids	1172:1199	four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group)	1172:1319	Moreover, relative abundance of two genera with LDA score &gt;3.5 (Megasphaera and LPS-producing Escherichia-Shigella), was significantly higher in AD subjects, while the abundance of four short chain fatty acids (SCFA) producing-genera (Christensenellaceae R-7, Ruminococcaceae UCG-014; Akkermansia and [Eubacterium] eligens group) was significantly higher in controls.					
36079803	2	88	theme	low	309:311	arg1	HDL-C					313:317	low HDL-C	309:317	low HDL-C	309:317	However, the relationship between the gut microbiota and atherogenic dyslipidemia (AD), defined as the presence of both low HDL-C and hypertriglyceridemia, is not fully understood.					
36079803	9	89	theme	polyunsaturated	1523:1537	arg1	PUFA/SFA					1572:1579	PUFA/SFA	1572:1579	PUFA/SFA	1572:1579	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	9	89	theme	polyunsaturated	1523:1537	arg1	acid					1566:1569	dietary polyunsaturated fatty acid/saturated fatty acid	1515:1569	dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio	1515:1586	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	5	90	theme	16S	900:902	arg1	rRNA					904:907	16S rRNA	900:907	16S rRNA	900:907	Gut microbiota composition was profiled using next-generation sequencing of 16S rRNA.					
36079803	9	91	theme	acid	1566:1569	arg1	ratio					1582:1586	dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio	1515:1586	dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio	1515:1586	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	9	92	theme	PUFA/SFA	1784:1791	arg1	ratio					1793:1797	PUFA/SFA ratio	1784:1797	PUFA/SFA ratio	1784:1797	Although dietary polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio and PUFA intake were higher in controls than in AD subjects, of the four genera differentiated in cases and controls, only Akkermansia abundance showed a positive and significant correlation with PUFA/SFA ratio.					
36079803	4	93	with	controls	653:660	arg1	AD					637:638	AD	637:638	AD	637:638	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
36079803	4	94	theme	abundance	705:713	arg1	correlations					682:693	correlations	682:693	correlations of genera abundance with serum lipid levels in 20 patients	682:752	We compared the microbial diversity and taxonomic composition in patients with AD (n = 41) and controls (n = 38) and sought correlations of genera abundance with serum lipid levels in 20 patients after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery.					
37004759	3	0	theme	self-administration	514:532	arg1	model					534:538	METH self-administration model	509:538	METH self-administration model	509:538	METHODS 16S rRNA gene sequencing was performed to assess the richness and diversity of gut microbiota in METH self-administration model.					
37004759	7	1	theme	receptor	946:953	arg1	levels					927:932	transcript levels	916:932	transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor	916:1029	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
37004759	8	2	theme	gut	1073:1075	arg1	dysbiosis					1088:1096	gut microbiota dysbiosis	1073:1096	gut microbiota dysbiosis	1073:1096	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	4	3	theme	intestinal	615:624	arg1	barrier					626:632	intestinal barrier	615:632	intestinal barrier	615:632	Hematoxylin and eosin staining was performed to evaluate the integrity of intestinal barrier.					
37004759	5	4	theme	morphologic	720:730	arg1	changes					732:738	the morphologic changes	716:738	the morphologic changes of microglia	716:751	Immunofluorescence and three-dimensional reconstruction were performed to assess the morphologic changes of microglia.					
37004759	13	5	theme	METH	2152:2155	arg1	craving					2157:2163	METH craving	2152:2163	METH craving	2152:2163	CONCLUSIONS These data suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse.					
37004759	13	6	theme	gram-negative	2042:2054	arg1	bacteria					2056:2063	gut gram-negative bacteria	2038:2063	gut gram-negative bacteria	2038:2063	CONCLUSIONS These data suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse.					
37004759	8	7	theme	dysbiosis	1088:1096	arg1	activation					1139:1148	gut microbiota dysbiosis, intestinal barrier damage and microglia activation	1073:1148	gut microbiota dysbiosis, intestinal barrier damage and microglia activation	1073:1148	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	10	8	theme	METH	1526:1529	arg1	craving					1531:1537	METH craving	1526:1537	METH craving	1526:1537	Depleting the gut microbiota also prevented the incubation of METH craving and increased the population of Klebsiella oxytoca.					
37004759	7	9	theme	transcript	916:925	arg1	levels					927:932	transcript levels	916:932	transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor	916:1029	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
37004759	1	10	theme	disorders	207:215	arg1	pathophysiology					171:185	the pathophysiology	167:185	the pathophysiology of neuropsychiatric disorders	167:215	BACKGROUND The microbiota-gut-brain axis plays a critical role in the pathophysiology of neuropsychiatric disorders, and the compositions of gut microbiota are altered by addictive drugs.					
37004759	8	11	theme	intestinal	1099:1108	arg1	damage					1118:1123	intestinal barrier damage	1099:1123	intestinal barrier damage	1099:1123	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	9	12	theme	marked	1327:1332	arg1	change					1334:1339	a marked change	1325:1339	a marked change in the microglial morphology in the NAcc	1325:1380	Microbiota depletion via antibiotic treatment increased LPS levels and induced a marked change in the microglial morphology in the NAcc, as indicated by the decreases in the lengths and numbers of microglial branches.					
37004759	9	13	from	decreases	1403:1411	arg1	numbers					1432:1438	the lengths and numbers	1416:1438	numbers	1432:1438	Microbiota depletion via antibiotic treatment increased LPS levels and induced a marked change in the microglial morphology in the NAcc, as indicated by the decreases in the lengths and numbers of microglial branches.					
37004759	9	13	from	decreases	1403:1411	arg1	lengths					1420:1426	the lengths and numbers	1416:1438	lengths	1420:1426	Microbiota depletion via antibiotic treatment increased LPS levels and induced a marked change in the microglial morphology in the NAcc, as indicated by the decreases in the lengths and numbers of microglial branches.					
37004759	8	14	theme	damage	1118:1123	arg1	activation					1139:1148	gut microbiota dysbiosis, intestinal barrier damage and microglia activation	1073:1148	gut microbiota dysbiosis, intestinal barrier damage and microglia activation	1073:1148	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	12	15	theme	bacterial	1914:1922	arg1	LPS					1924:1926	gut-derived bacterial LPS	1902:1926	gut-derived bacterial LPS	1902:1926	Both treatments and NAcc microinjections of gut-derived bacterial LPS significantly decreased METH craving after prolonged withdrawal.					
37004759	2	16	theme	METH	363:366	arg1	craving					369:375	methamphetamine (METH) craving	346:375	methamphetamine (METH) craving	346:375	However, the role of gut microbiota in the incubation of methamphetamine (METH) craving remains poorly understood.					
37004759	9	17	theme	microglial	1348:1357	arg1	morphology					1359:1368	the microglial morphology	1344:1368	the microglial morphology	1344:1368	Microbiota depletion via antibiotic treatment increased LPS levels and induced a marked change in the microglial morphology in the NAcc, as indicated by the decreases in the lengths and numbers of microglial branches.					
37004759	1	18	theme	gut	242:244	arg1	microbiota					246:255	gut microbiota	242:255	gut microbiota	242:255	BACKGROUND The microbiota-gut-brain axis plays a critical role in the pathophysiology of neuropsychiatric disorders, and the compositions of gut microbiota are altered by addictive drugs.					
37004759	11	19	theme	microglial	1764:1773	arg1	changes					1789:1795	microglial morphological changes	1764:1795	microglial morphological changes	1764:1795	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	11	20	theme	gram-negative	1668:1680	arg1	LPS					1712:1714	the gram-negative bacterial cell wall component LPS	1664:1714	the gram-negative bacterial cell wall component LPS	1664:1714	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	7	21	link	brain-derived	997:1009	arg1	factor					1024:1029	brain-derived neurotrophic factor	997:1029	brain-derived neurotrophic factor	997:1029	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
37004759	12	22	theme	gut-derived	1902:1912	arg1	LPS					1924:1926	gut-derived bacterial LPS	1902:1926	gut-derived bacterial LPS	1902:1926	Both treatments and NAcc microinjections of gut-derived bacterial LPS significantly decreased METH craving after prolonged withdrawal.					
37004759	1	23	dep	BACKGROUND	101:110	arg1	altered					261:267	altered	261:267	are altered by addictive drugs	257:286	BACKGROUND The microbiota-gut-brain axis plays a critical role in the pathophysiology of neuropsychiatric disorders, and the compositions of gut microbiota are altered by addictive drugs.					
37004759	1	23	dep	BACKGROUND	101:110	arg1	plays					142:146	plays	142:146	plays a critical role in the pathophysiology of neuropsychiatric disorders	142:215	BACKGROUND The microbiota-gut-brain axis plays a critical role in the pathophysiology of neuropsychiatric disorders, and the compositions of gut microbiota are altered by addictive drugs.					
37004759	11	24	theme	exogenous	1636:1644	arg1	administration					1646:1659	exogenous administration	1636:1659	exogenous administration of the gram-negative bacterial cell wall component LPS	1636:1714	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	12	25	link	gut-derived	1902:1912	arg1	LPS					1924:1926	gut-derived bacterial LPS	1902:1926	gut-derived bacterial LPS	1902:1926	Both treatments and NAcc microinjections of gut-derived bacterial LPS significantly decreased METH craving after prolonged withdrawal.					
37004759	8	26	theme	microglia	1129:1137	arg1	activation					1139:1148	gut microbiota dysbiosis, intestinal barrier damage and microglia activation	1073:1148	gut microbiota dysbiosis, intestinal barrier damage and microglia activation	1073:1148	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	13	27	theme	circulating	2075:2085	arg1	blood					2087:2091	circulating blood	2075:2091	circulating blood	2075:2091	CONCLUSIONS These data suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse.					
37004759	10	28	theme	oxytoca	1582:1588	arg1	population					1557:1566	the population	1553:1566	the population of Klebsiella oxytoca	1553:1588	Depleting the gut microbiota also prevented the incubation of METH craving and increased the population of Klebsiella oxytoca.					
37004759	8	29	theme	nucleus	1157:1163	arg1	accumbens					1165:1173	nucleus accumbens	1157:1173	the nucleus accumbens core (NAcc)	1153:1185	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	7	30	theme	factor	1024:1029	arg1	levels					927:932	transcript levels	916:932	transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor	916:1029	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
37004759	7	31	theme	Quantitative	865:876	arg1	PCR					888:890	Quantitative real-time PCR	865:890	Quantitative real-time PCR	865:890	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
37004759	6	32	theme	Serum	754:758	arg1	levels					760:765	Serum levels	754:765	Serum levels of lipopolysaccharide (LPS)	754:793	Serum levels of lipopolysaccharide (LPS) were determined using the rat enzyme-linked immunosorbent assay kits.					
37004759	0	33	theme	Gut-derived	0:10	arg1	LPS					22:24	Gut-derived bacterial LPS	0:24	Gut-derived bacterial LPS	0:24	Gut-derived bacterial LPS attenuates incubation of methamphetamine craving via modulating microglia.					
37004759	7	34	theme	real-time	878:886	arg1	PCR					888:890	Quantitative real-time PCR	865:890	Quantitative real-time PCR	865:890	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
37004759	7	35	theme	brain-derived	997:1009	arg1	factor					1024:1029	brain-derived neurotrophic factor	997:1029	brain-derived neurotrophic factor	997:1029	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
37004759	13	36	from	microglia	2103:2111	arg1	brain					2120:2124	the brain	2116:2124	the brain	2116:2124	CONCLUSIONS These data suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse.					
37004759	0	37	link	Gut-derived	0:10	arg1	LPS					22:24	Gut-derived bacterial LPS	0:24	Gut-derived bacterial LPS	0:24	Gut-derived bacterial LPS attenuates incubation of methamphetamine craving via modulating microglia.					
37004759	13	38	dep	CONCLUSIONS	1993:2003	arg1	suggest					2016:2022	suggest	2016:2022	suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse	2016:2278	CONCLUSIONS These data suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse.					
37004759	3	39	theme	gene	421:424	arg1	sequencing					426:435	rRNA gene sequencing	416:435	rRNA gene sequencing	416:435	METHODS 16S rRNA gene sequencing was performed to assess the richness and diversity of gut microbiota in METH self-administration model.					
37004759	9	40	theme	branches	1454:1461	arg1	numbers					1432:1438	the lengths and numbers	1416:1438	numbers	1432:1438	Microbiota depletion via antibiotic treatment increased LPS levels and induced a marked change in the microglial morphology in the NAcc, as indicated by the decreases in the lengths and numbers of microglial branches.					
37004759	9	40	theme	branches	1454:1461	arg1	lengths					1420:1426	the lengths and numbers	1416:1438	lengths	1420:1426	Microbiota depletion via antibiotic treatment increased LPS levels and induced a marked change in the microglial morphology in the NAcc, as indicated by the decreases in the lengths and numbers of microglial branches.					
37004759	4	41	theme	Hematoxylin	541:551	arg1	staining					563:570	Hematoxylin and eosin staining	541:570	Hematoxylin and eosin staining	541:570	Hematoxylin and eosin staining was performed to evaluate the integrity of intestinal barrier.					
37004759	3	42	from	richness	465:472	arg1	model					534:538	METH self-administration model	509:538	METH self-administration model	509:538	METHODS 16S rRNA gene sequencing was performed to assess the richness and diversity of gut microbiota in METH self-administration model.					
37004759	3	43	theme	microbiota	495:504	arg1	richness					465:472	richness	465:472	richness	465:472	METHODS 16S rRNA gene sequencing was performed to assess the richness and diversity of gut microbiota in METH self-administration model.					
37004759	3	43	theme	microbiota	495:504	arg1	diversity					478:486	diversity	478:486	diversity	478:486	METHODS 16S rRNA gene sequencing was performed to assess the richness and diversity of gut microbiota in METH self-administration model.					
37004759	0	44	theme	methamphetamine	51:65	arg1	craving					67:73	methamphetamine craving	51:73	methamphetamine craving	51:73	Gut-derived bacterial LPS attenuates incubation of methamphetamine craving via modulating microglia.					
37004759	4	45	theme	eosin	557:561	arg1	staining					563:570	Hematoxylin and eosin staining	541:570	Hematoxylin and eosin staining	541:570	Hematoxylin and eosin staining was performed to evaluate the integrity of intestinal barrier.					
37004759	11	46	theme	wall	1697:1700	arg1	LPS					1712:1714	the gram-negative bacterial cell wall component LPS	1664:1714	the gram-negative bacterial cell wall component LPS	1664:1714	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	3	47	from	diversity	478:486	arg1	model					534:538	METH self-administration model	509:538	METH self-administration model	509:538	METHODS 16S rRNA gene sequencing was performed to assess the richness and diversity of gut microbiota in METH self-administration model.					
37004759	1	48	theme	microbiota	246:255	arg1	compositions					226:237	the compositions	222:237	the compositions of gut microbiota	222:255	BACKGROUND The microbiota-gut-brain axis plays a critical role in the pathophysiology of neuropsychiatric disorders, and the compositions of gut microbiota are altered by addictive drugs.					
37004759	8	49	theme	METH	1040:1043	arg1	self-administration					1045:1063	RESULTS METH self-administration	1032:1063	RESULTS METH self-administration	1032:1063	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	4	50	theme	barrier	626:632	arg1	integrity					602:610	the integrity	598:610	the integrity of intestinal barrier	598:632	Hematoxylin and eosin staining was performed to evaluate the integrity of intestinal barrier.					
37004759	7	51	theme	receptor	982:989	arg1	levels					927:932	transcript levels	916:932	transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor	916:1029	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
37004759	8	52	theme	prolonged	1224:1232	arg1	withdrawal					1234:1243	prolonged withdrawal	1224:1243	prolonged withdrawal	1224:1243	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	11	53	theme	LPS	1744:1746	arg1	levels					1748:1753	central LPS levels	1736:1753	central LPS levels	1736:1753	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	3	54	theme	METH	509:512	arg1	model					534:538	METH self-administration model	509:538	METH self-administration model	509:538	METHODS 16S rRNA gene sequencing was performed to assess the richness and diversity of gut microbiota in METH self-administration model.					
37004759	7	55	theme	ionotropic	966:975	arg1	receptor					982:989	glutamate ionotropic AMPA receptor 3	956:991	glutamate ionotropic AMPA receptor 3	956:991	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
37004759	1	56	theme	addictive	272:280	arg1	drugs					282:286	addictive drugs	272:286	addictive drugs	272:286	BACKGROUND The microbiota-gut-brain axis plays a critical role in the pathophysiology of neuropsychiatric disorders, and the compositions of gut microbiota are altered by addictive drugs.					
37004759	8	57	theme	microbiota	1077:1086	arg1	dysbiosis					1088:1096	gut microbiota dysbiosis	1073:1096	gut microbiota dysbiosis	1073:1096	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	11	58	theme	component	1702:1710	arg1	LPS					1712:1714	the gram-negative bacterial cell wall component LPS	1664:1714	the gram-negative bacterial cell wall component LPS	1664:1714	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	9	59	theme	LPS	1302:1304	arg1	levels					1306:1311	LPS levels	1302:1311	LPS levels	1302:1311	Microbiota depletion via antibiotic treatment increased LPS levels and induced a marked change in the microglial morphology in the NAcc, as indicated by the decreases in the lengths and numbers of microglial branches.					
37004759	6	60	link	enzyme-linked	825:837	arg1	assay					853:857	the rat enzyme-linked immunosorbent assay	817:857	the rat enzyme-linked immunosorbent assay kits	817:862	Serum levels of lipopolysaccharide (LPS) were determined using the rat enzyme-linked immunosorbent assay kits.					
37004759	11	61	theme	dopamine	1813:1820	arg1	receptor					1822:1829	the dopamine receptor	1809:1829	the dopamine receptor transcription in the NAcc	1809:1855	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	5	62	theme	three-dimensional	658:674	arg1	reconstruction					676:689	three-dimensional reconstruction	658:689	three-dimensional reconstruction	658:689	Immunofluorescence and three-dimensional reconstruction were performed to assess the morphologic changes of microglia.					
37004759	10	63	theme	gut	1478:1480	arg1	microbiota					1482:1491	the gut microbiota	1474:1491	the gut microbiota	1474:1491	Depleting the gut microbiota also prevented the incubation of METH craving and increased the population of Klebsiella oxytoca.					
37004759	7	64	theme	dopamine	937:944	arg1	receptor					946:953	dopamine receptor	937:953	dopamine receptor	937:953	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
37004759	1	65	theme	neuropsychiatric	190:205	arg1	disorders					207:215	neuropsychiatric disorders	190:215	neuropsychiatric disorders	190:215	BACKGROUND The microbiota-gut-brain axis plays a critical role in the pathophysiology of neuropsychiatric disorders, and the compositions of gut microbiota are altered by addictive drugs.					
37004759	8	66	theme	barrier	1110:1116	arg1	damage					1118:1123	intestinal barrier damage	1099:1123	intestinal barrier damage	1099:1123	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	6	67	theme	assay	853:857	arg1	kits					859:862	the rat enzyme-linked immunosorbent assay kits	817:862	the rat enzyme-linked immunosorbent assay kits	817:862	Serum levels of lipopolysaccharide (LPS) were determined using the rat enzyme-linked immunosorbent assay kits.					
37004759	6	68	theme	immunosorbent	839:851	arg1	assay					853:857	the rat enzyme-linked immunosorbent assay	817:857	the rat enzyme-linked immunosorbent assay kits	817:862	Serum levels of lipopolysaccharide (LPS) were determined using the rat enzyme-linked immunosorbent assay kits.					
37004759	6	69	theme	enzyme-linked	825:837	arg1	assay					853:857	the rat enzyme-linked immunosorbent assay	817:857	the rat enzyme-linked immunosorbent assay kits	817:862	Serum levels of lipopolysaccharide (LPS) were determined using the rat enzyme-linked immunosorbent assay kits.					
37004759	2	70	theme	microbiota	314:323	arg1	role					302:305	the role	298:305	the role of gut microbiota in the incubation of methamphetamine (METH) craving	298:375	However, the role of gut microbiota in the incubation of methamphetamine (METH) craving remains poorly understood.					
37004759	13	71	theme	important	2198:2206	arg1	implications					2208:2219	important implications	2198:2219	important implications for novel strategies to prevent METH addiction and relapse	2198:2278	CONCLUSIONS These data suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse.					
37004759	11	72	theme	bacterial	1682:1690	arg1	LPS					1712:1714	the gram-negative bacterial cell wall component LPS	1664:1714	the gram-negative bacterial cell wall component LPS	1664:1714	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	9	73	from	change	1334:1339	arg1	morphology					1359:1368	the microglial morphology	1344:1368	the microglial morphology	1344:1368	Microbiota depletion via antibiotic treatment increased LPS levels and induced a marked change in the microglial morphology in the NAcc, as indicated by the decreases in the lengths and numbers of microglial branches.					
37004759	9	73	from	change	1334:1339	arg1	NAcc					1377:1380	the NAcc	1373:1380	the NAcc	1373:1380	Microbiota depletion via antibiotic treatment increased LPS levels and induced a marked change in the microglial morphology in the NAcc, as indicated by the decreases in the lengths and numbers of microglial branches.					
37004759	11	74	theme	cell	1692:1695	arg1	LPS					1712:1714	the gram-negative bacterial cell wall component LPS	1664:1714	the gram-negative bacterial cell wall component LPS	1664:1714	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	5	75	theme	microglia	743:751	arg1	changes					732:738	the morphologic changes	716:738	the morphologic changes of microglia	716:751	Immunofluorescence and three-dimensional reconstruction were performed to assess the morphologic changes of microglia.					
37004759	1	76	theme	critical	150:157	arg1	role					159:162	a critical role	148:162	a critical role	148:162	BACKGROUND The microbiota-gut-brain axis plays a critical role in the pathophysiology of neuropsychiatric disorders, and the compositions of gut microbiota are altered by addictive drugs.					
37004759	9	77	theme	Microbiota	1246:1255	arg1	depletion					1257:1265	Microbiota depletion	1246:1265	Microbiota depletion via antibiotic treatment	1246:1290	Microbiota depletion via antibiotic treatment increased LPS levels and induced a marked change in the microglial morphology in the NAcc, as indicated by the decreases in the lengths and numbers of microglial branches.					
37004759	12	78	theme	NAcc	1878:1881	arg1	microinjections					1883:1897	NAcc microinjections	1878:1897	NAcc microinjections	1878:1897	Both treatments and NAcc microinjections of gut-derived bacterial LPS significantly decreased METH craving after prolonged withdrawal.					
37004759	12	79	theme	METH	1952:1955	arg1	craving					1957:1963	METH craving	1952:1963	METH craving	1952:1963	Both treatments and NAcc microinjections of gut-derived bacterial LPS significantly decreased METH craving after prolonged withdrawal.					
37004759	2	80	from	role	302:305	arg1	incubation					332:341	the incubation	328:341	the incubation of methamphetamine (METH) craving	328:375	However, the role of gut microbiota in the incubation of methamphetamine (METH) craving remains poorly understood.					
37004759	13	81	theme	METH	2253:2256	arg1	addiction					2258:2266	METH addiction	2253:2266	METH addiction	2253:2266	CONCLUSIONS These data suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse.					
37004759	11	82	theme	oxytoca	1615:1621	arg1	treatment					1623:1631	Klebsiella oxytoca treatment	1604:1631	Klebsiella oxytoca treatment	1604:1631	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	2	83	theme	gut	310:312	arg1	microbiota					314:323	gut microbiota	310:323	gut microbiota	310:323	However, the role of gut microbiota in the incubation of methamphetamine (METH) craving remains poorly understood.					
37004759	11	84	from	transcription	1831:1843	arg1	NAcc					1852:1855	the NAcc	1848:1855	the NAcc	1848:1855	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	10	85	theme	craving	1531:1537	arg1	incubation					1512:1521	the incubation	1508:1521	the incubation of METH craving	1508:1537	Depleting the gut microbiota also prevented the incubation of METH craving and increased the population of Klebsiella oxytoca.					
37004759	6	86	theme	lipopolysaccharide	770:787	arg1	levels					760:765	Serum levels	754:765	Serum levels of lipopolysaccharide (LPS)	754:793	Serum levels of lipopolysaccharide (LPS) were determined using the rat enzyme-linked immunosorbent assay kits.					
37004759	13	87	theme	novel	2225:2229	arg1	strategies					2231:2240	novel strategies to prevent METH addiction and relapse	2225:2278	novel strategies to prevent METH addiction and relapse	2225:2278	CONCLUSIONS These data suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse.					
37004759	8	88	theme	accumbens	1165:1173	arg1	NAcc					1181:1184	NAcc	1181:1184	NAcc	1181:1184	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	8	88	theme	accumbens	1165:1173	arg1	core					1175:1178	the nucleus accumbens core	1153:1178	the nucleus accumbens core (NAcc)	1153:1185	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	13	89	from	bacteria	2056:2063	arg1	LPS					2029:2031	LPS	2029:2031	LPS from gut gram-negative bacteria	2029:2063	CONCLUSIONS These data suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse.					
37004759	10	90	theme	Klebsiella	1571:1580	arg1	oxytoca					1582:1588	Klebsiella oxytoca	1571:1588	Klebsiella oxytoca	1571:1588	Depleting the gut microbiota also prevented the incubation of METH craving and increased the population of Klebsiella oxytoca.					
37004759	12	91	theme	LPS	1924:1926	arg1	microinjections					1883:1897	NAcc microinjections	1878:1897	NAcc microinjections	1878:1897	Both treatments and NAcc microinjections of gut-derived bacterial LPS significantly decreased METH craving after prolonged withdrawal.					
37004759	12	91	theme	LPS	1924:1926	arg1	treatments					1863:1872	treatments	1863:1872	treatments	1863:1872	Both treatments and NAcc microinjections of gut-derived bacterial LPS significantly decreased METH craving after prolonged withdrawal.					
37004759	0	92	theme	bacterial	12:20	arg1	LPS					22:24	Gut-derived bacterial LPS	0:24	Gut-derived bacterial LPS	0:24	Gut-derived bacterial LPS attenuates incubation of methamphetamine craving via modulating microglia.					
37004759	7	93	theme	neurotrophic	1011:1022	arg1	factor					1024:1029	brain-derived neurotrophic factor	997:1029	brain-derived neurotrophic factor	997:1029	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
37004759	1	94	theme	microbiota-gut-brain	116:135	arg1	axis					137:140	The microbiota-gut-brain axis	112:140	The microbiota-gut-brain axis	112:140	BACKGROUND The microbiota-gut-brain axis plays a critical role in the pathophysiology of neuropsychiatric disorders, and the compositions of gut microbiota are altered by addictive drugs.					
37004759	9	95	theme	microglial	1443:1452	arg1	branches					1454:1461	microglial branches	1443:1461	microglial branches	1443:1461	Microbiota depletion via antibiotic treatment increased LPS levels and induced a marked change in the microglial morphology in the NAcc, as indicated by the decreases in the lengths and numbers of microglial branches.					
37004759	11	96	theme	receptor	1822:1829	arg1	transcription					1831:1843	the dopamine receptor transcription	1809:1843	the dopamine receptor transcription in the NAcc	1809:1855	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	6	97	theme	rat	821:823	arg1	assay					853:857	the rat enzyme-linked immunosorbent assay	817:857	the rat enzyme-linked immunosorbent assay kits	817:862	Serum levels of lipopolysaccharide (LPS) were determined using the rat enzyme-linked immunosorbent assay kits.					
37004759	0	98	theme	craving	67:73	arg1	incubation					37:46	incubation	37:46	incubation of methamphetamine craving	37:73	Gut-derived bacterial LPS attenuates incubation of methamphetamine craving via modulating microglia.					
37004759	12	99	theme	prolonged	1971:1979	arg1	withdrawal					1981:1990	prolonged withdrawal	1971:1990	prolonged withdrawal	1971:1990	Both treatments and NAcc microinjections of gut-derived bacterial LPS significantly decreased METH craving after prolonged withdrawal.					
37004759	3	100	theme	rRNA	416:419	arg1	sequencing					426:435	rRNA gene sequencing	416:435	rRNA gene sequencing	416:435	METHODS 16S rRNA gene sequencing was performed to assess the richness and diversity of gut microbiota in METH self-administration model.					
37004759	11	101	theme	LPS	1712:1714	arg1	treatment					1623:1631	Klebsiella oxytoca treatment	1604:1631	Klebsiella oxytoca treatment	1604:1631	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	11	101	theme	LPS	1712:1714	arg1	administration					1646:1659	exogenous administration	1636:1659	exogenous administration of the gram-negative bacterial cell wall component LPS	1636:1714	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	2	102	theme	methamphetamine	346:360	arg1	craving					369:375	methamphetamine (METH) craving	346:375	methamphetamine (METH) craving	346:375	However, the role of gut microbiota in the incubation of methamphetamine (METH) craving remains poorly understood.					
37004759	11	103	theme	central	1736:1742	arg1	levels					1748:1753	central LPS levels	1736:1753	central LPS levels	1736:1753	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	3	104	theme	gut	491:493	arg1	microbiota					495:504	gut microbiota	491:504	gut microbiota	491:504	METHODS 16S rRNA gene sequencing was performed to assess the richness and diversity of gut microbiota in METH self-administration model.					
37004759	7	105	theme	AMPA	977:980	arg1	receptor					982:989	glutamate ionotropic AMPA receptor 3	956:991	glutamate ionotropic AMPA receptor 3	956:991	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
37004759	13	106	theme	gut	2038:2040	arg1	bacteria					2056:2063	gut gram-negative bacteria	2038:2063	gut gram-negative bacteria	2038:2063	CONCLUSIONS These data suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse.					
37004759	8	107	theme	RESULTS	1032:1038	arg1	self-administration					1045:1063	RESULTS METH self-administration	1032:1063	RESULTS METH self-administration	1032:1063	RESULTS METH self-administration induced gut microbiota dysbiosis, intestinal barrier damage and microglia activation in the nucleus accumbens core (NAcc), which was partially recovered after prolonged withdrawal.					
37004759	13	108	contain	have	2193:2196	arg2	implications					2208:2219	important implications	2198:2219	important implications for novel strategies to prevent METH addiction and relapse	2198:2278	CONCLUSIONS These data suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse.					
37004759	13	108	contain	have	2193:2196	arg1	withdrawal					2171:2180	withdrawal	2171:2180	withdrawal	2171:2180	CONCLUSIONS These data suggest that LPS from gut gram-negative bacteria may enter circulating blood, activate microglia in the brain and consequently decrease METH craving after withdrawal, which may have important implications for novel strategies to prevent METH addiction and relapse.					
37004759	11	109	theme	morphological	1775:1787	arg1	changes					1789:1795	microglial morphological changes	1764:1795	microglial morphological changes	1764:1795	Furthermore, Klebsiella oxytoca treatment or exogenous administration of the gram-negative bacterial cell wall component LPS increased serum and central LPS levels, induced microglial morphological changes and reduced the dopamine receptor transcription in the NAcc.					
37004759	9	110	theme	antibiotic	1271:1280	arg1	treatment					1282:1290	antibiotic treatment	1271:1290	antibiotic treatment	1271:1290	Microbiota depletion via antibiotic treatment increased LPS levels and induced a marked change in the microglial morphology in the NAcc, as indicated by the decreases in the lengths and numbers of microglial branches.					
37004759	2	111	theme	craving	369:375	arg1	incubation					332:341	the incubation	328:341	the incubation of methamphetamine (METH) craving	328:375	However, the role of gut microbiota in the incubation of methamphetamine (METH) craving remains poorly understood.					
37004759	7	112	theme	glutamate	956:964	arg1	receptor					982:989	glutamate ionotropic AMPA receptor 3	956:991	glutamate ionotropic AMPA receptor 3	956:991	Quantitative real-time PCR was performed to assess transcript levels of dopamine receptor, glutamate ionotropic AMPA receptor 3 and brain-derived neurotrophic factor.					
36813813	3	0	from	effect	366:371	arg1	interaction					415:425	the interaction	411:425	the interaction between CNTs and glycoproteins	411:456	We examine the effect of carbon nanotube (CNT) chirality on the interaction between CNTs and glycoproteins.					
36813813	1	1	link	N-linked	182:189	arg1	glycoproteins					221:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	5	2	theme	N-linked	699:706	arg1	presence					687:694	the presence	683:694	the presence of N-linked	683:706	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	7	3	theme	spike	1032:1036	arg1	protein					1038:1044	the spike protein	1028:1044	the spike protein	1028:1044	Thereby, we predict CNBs and chiral CNTs have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein.					
36813813	7	4	theme	N-	995:996	arg1	analysis					983:990	sequential analysis	972:990	sequential analysis of N- and O-linked glycosylation of the spike protein	972:1044	Thereby, we predict CNBs and chiral CNTs have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein.					
36813813	7	5	theme	protein	1038:1044	arg1	N-					995:996	N-	995:996	N-	995:996	Thereby, we predict CNBs and chiral CNTs have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein.					
36813813	7	5	theme	protein	1038:1044	arg1	glycosylation					1011:1023	O-linked glycosylation	1002:1023	O-linked glycosylation	1002:1023	Thereby, we predict CNBs and chiral CNTs have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein.					
36813813	5	6	theme	CNTs	665:668	arg1	gaps					675:678	the CNTs band gaps	661:678	the CNTs band gaps in the presence of N-linked	661:706	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	7	7	theme	glycosylation	1011:1023	arg1	analysis					983:990	sequential analysis	972:990	sequential analysis of N- and O-linked glycosylation of the spike protein	972:1044	Thereby, we predict CNBs and chiral CNTs have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein.					
36813813	1	8	theme	ab	242:243	arg1	initio					245:250	ab initio	242:250	ab initio quantum mechanical approach	242:278	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	5	9	gly	glycoproteins	848:860	arg1	glycoproteins					848:860	the glycoproteins	844:860	the glycoproteins	844:860	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	5	10	gly	glycoprotein	784:795	arg1	glycoprotein					784:795	the O-linked glycoprotein	771:795	the O-linked glycoprotein	771:795	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	1	11	gly	glycoproteins	221:233	arg1	glycoproteins					221:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	1	12	theme	initio	245:250	arg1	approach					271:278	ab initio quantum mechanical approach	242:278	ab initio quantum mechanical approach	242:278	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	4	13	theme	electron	606:613	arg1	density					615:621	electron density	606:621	electron density of states (DOS)	606:637	Results indicate that the chiral semiconductor CNTs clearly response to the presence of the glycoproteins by changing the electronic band gaps and electron density of states (DOS).					
36813813	5	14	theme	glycoprotein	784:795	arg1	presence					759:766	the presence	755:766	the presence of the O-linked glycoprotein	755:795	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	1	15	theme	carbon	157:162	arg1	CNB					174:176	CNB	174:176	CNB	174:176	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	1	15	theme	carbon	157:162	arg1	nanobelt					164:171	a carbon nanobelt	155:171	a carbon nanobelt (CNB)	155:177	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	5	16	from	changes	650:656	arg1	gaps					675:678	the CNTs band gaps	661:678	the CNTs band gaps in the presence of N-linked	661:706	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	5	17	dep	times	722:726	arg1	larger					728:733	larger	728:733	larger	728:733	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	5	18	theme	glycoproteins	848:860	arg1	types					835:839	different types	825:839	different types of the glycoproteins	825:860	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	7	19	dep	CNBs	924:927	arg1	have					945:948	have	945:948	have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein	945:1044	Thereby, we predict CNBs and chiral CNTs have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein.					
36813813	1	20	theme	nanobelt	164:171	arg1	response					99:106	the electronic response	84:106	the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins	84:233	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	3	21	gly	glycoproteins	444:456	arg1	glycoproteins					444:456	glycoproteins	444:456	glycoproteins	444:456	We examine the effect of carbon nanotube (CNT) chirality on the interaction between CNTs and glycoproteins.					
36813813	5	22	from	changes	744:750	arg1	presence					759:766	the presence	755:766	the presence of the O-linked glycoprotein	755:795	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	3	23	theme	nanotube	383:390	arg1	chirality					398:406	carbon nanotube (CNT) chirality	376:406	carbon nanotube (CNT) chirality	376:406	We examine the effect of carbon nanotube (CNT) chirality on the interaction between CNTs and glycoproteins.					
36813813	1	24	theme	quantum	252:258	arg1	approach					271:278	ab initio quantum mechanical approach	242:278	ab initio quantum mechanical approach	242:278	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	5	25	link	O-linked	775:782	arg1	glycoprotein					784:795	the O-linked glycoprotein	771:795	the O-linked glycoprotein	771:795	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	1	26	link	O-linked	195:202	arg1	glycoproteins					221:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	1	27	theme	electronic	88:97	arg1	response					99:106	the electronic response	84:106	the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins	84:233	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	4	28	theme	states	626:631	arg1	gaps					597:600	the electronic band gaps	577:600	the electronic band gaps	577:600	Results indicate that the chiral semiconductor CNTs clearly response to the presence of the glycoproteins by changing the electronic band gaps and electron density of states (DOS).					
36813813	4	28	theme	states	626:631	arg1	density					615:621	electron density	606:621	electron density of states (DOS)	606:637	Results indicate that the chiral semiconductor CNTs clearly response to the presence of the glycoproteins by changing the electronic band gaps and electron density of states (DOS).					
36813813	1	29	theme	mechanical	260:269	arg1	approach					271:278	ab initio quantum mechanical approach	242:278	ab initio quantum mechanical approach	242:278	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	7	30	theme	sequential	972:981	arg1	analysis					983:990	sequential analysis	972:990	sequential analysis of N- and O-linked glycosylation of the spike protein	972:1044	Thereby, we predict CNBs and chiral CNTs have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein.					
36813813	4	31	gly	glycoproteins	551:563	arg1	glycoproteins					551:563	the glycoproteins	547:563	the glycoproteins	547:563	Results indicate that the chiral semiconductor CNTs clearly response to the presence of the glycoproteins by changing the electronic band gaps and electron density of states (DOS).					
36813813	5	32	from	gaps	675:678	arg1	presence					687:694	the presence	683:694	the presence of N-linked	683:706	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	4	33	theme	band	592:595	arg1	gaps					597:600	the electronic band gaps	577:600	the electronic band gaps	577:600	Results indicate that the chiral semiconductor CNTs clearly response to the presence of the glycoproteins by changing the electronic band gaps and electron density of states (DOS).					
36813813	0	34	theme	potential	34:42	arg1	materials					44:52	potential materials	34:52	potential materials for biosensor	34:66	Carbon nanotubes and nanobelts as potential materials for biosensor.					
36813813	3	35	theme	chirality	398:406	arg1	effect					366:371	the effect	362:371	the effect of carbon nanotube (CNT) chirality on the interaction between CNTs and glycoproteins	362:456	We examine the effect of carbon nanotube (CNT) chirality on the interaction between CNTs and glycoproteins.					
36813813	7	36	link	O-linked	1002:1009	arg1	glycosylation					1011:1023	O-linked glycosylation	1002:1023	O-linked glycosylation	1002:1023	Thereby, we predict CNBs and chiral CNTs have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein.					
36813813	1	37	theme	N-linked	182:189	arg1	glycoproteins					221:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	3	38	theme	carbon	376:381	arg1	CNT					393:395	CNT	393:395	CNT	393:395	We examine the effect of carbon nanotube (CNT) chirality on the interaction between CNTs and glycoproteins.					
36813813	3	38	theme	carbon	376:381	arg1	nanotube					383:390	carbon nanotube	376:390	carbon nanotube (CNT) chirality	376:406	We examine the effect of carbon nanotube (CNT) chirality on the interaction between CNTs and glycoproteins.					
36813813	4	39	theme	chiral	485:490	arg1	CNTs					506:509	the chiral semiconductor CNTs	481:509	the chiral semiconductor CNTs	481:509	Results indicate that the chiral semiconductor CNTs clearly response to the presence of the glycoproteins by changing the electronic band gaps and electron density of states (DOS).					
36813813	4	40	theme	electronic	581:590	arg1	gaps					597:600	the electronic band gaps	577:600	the electronic band gaps	577:600	Results indicate that the chiral semiconductor CNTs clearly response to the presence of the glycoproteins by changing the electronic band gaps and electron density of states (DOS).					
36813813	7	41	gly	glycosylation	1011:1023	arg1	protein					1038:1044	the spike protein	1028:1044	the spike protein	1028:1044	Thereby, we predict CNBs and chiral CNTs have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein.					
36813813	5	42	theme	two	718:720	arg1	changes					650:656	the changes	646:656	the changes in the CNTs band gaps in the presence of N-linked	646:706	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	5	42	theme	two	718:720	arg1	times					722:726	times	722:726	times	722:726	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	5	43	theme	chiral	798:803	arg1	CNT					805:807	chiral CNT	798:807	chiral CNT	798:807	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	7	44	theme	chiral	933:938	arg1	CNTs					940:943	chiral CNTs	933:943	chiral CNTs	933:943	Thereby, we predict CNBs and chiral CNTs have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein.					
36813813	1	45	theme	single-walled	111:123	arg1	nanotubes					132:140	single-walled carbon nanotubes	111:140	single-walled carbon nanotubes (SWCNTs)	111:149	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	1	45	theme	single-walled	111:123	arg1	SWCNTs					143:148	SWCNTs	143:148	SWCNTs	143:148	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	7	46	theme	suitable	950:957	arg1	potential					959:967	suitable potential	950:967	suitable potential	950:967	Thereby, we predict CNBs and chiral CNTs have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein.					
36813813	7	47	theme	O-linked	1002:1009	arg1	glycosylation					1011:1023	O-linked glycosylation	1002:1023	O-linked glycosylation	1002:1023	Thereby, we predict CNBs and chiral CNTs have suitable potential in sequential analysis of N- and O-linked glycosylation of the spike protein.					
36813813	1	48	theme	carbon	125:130	arg1	nanotubes					132:140	single-walled carbon nanotubes	111:140	single-walled carbon nanotubes (SWCNTs)	111:149	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	1	48	theme	carbon	125:130	arg1	SWCNTs					143:148	SWCNTs	143:148	SWCNTs	143:148	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	4	49	theme	semiconductor	492:504	arg1	CNTs					506:509	the chiral semiconductor CNTs	481:509	the chiral semiconductor CNTs	481:509	Results indicate that the chiral semiconductor CNTs clearly response to the presence of the glycoproteins by changing the electronic band gaps and electron density of states (DOS).					
36813813	6	50	theme	same	867:870	arg1	results					872:878	The same results	863:878	The same results	863:878	The same results are obtained from CNBs.					
36813813	1	51	theme	nanotubes	132:140	arg1	response					99:106	the electronic response	84:106	the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins	84:233	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	1	52	theme	O-linked	195:202	arg1	glycoproteins					221:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	5	53	theme	different	825:833	arg1	types					835:839	different types	825:839	different types of the glycoproteins	825:860	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	1	54	theme	SARS-CoV-2	204:213	arg1	glycoproteins					221:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	2	55	theme	chiral	336:341	arg1	groups					343:348	chiral groups	336:348	chiral groups	336:348	The CNTs are selected from three zigzag, armchair, and chiral groups.					
36813813	5	56	theme	O-linked	775:782	arg1	glycoprotein					784:795	the O-linked glycoprotein	771:795	the O-linked glycoprotein	771:795	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
36813813	1	57	theme	spike	215:219	arg1	glycoproteins					221:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	N-linked and O-linked SARS-CoV-2 spike glycoproteins	182:233	We investigate the electronic response of single-walled carbon nanotubes (SWCNTs) and a carbon nanobelt (CNB) to N-linked and O-linked SARS-CoV-2 spike glycoproteins, using ab initio quantum mechanical approach.					
36813813	4	58	theme	glycoproteins	551:563	arg1	presence					535:542	the presence	531:542	the presence of the glycoproteins	531:563	Results indicate that the chiral semiconductor CNTs clearly response to the presence of the glycoproteins by changing the electronic band gaps and electron density of states (DOS).					
36813813	5	59	theme	band	670:673	arg1	gaps					675:678	the CNTs band gaps	661:678	the CNTs band gaps in the presence of N-linked	661:706	Since the changes in the CNTs band gaps in the presence of N-linked are about two times larger than the changes in the presence of the O-linked glycoprotein, chiral CNT may distinguish different types of the glycoproteins.					
35995297	0	0	theme	oral	73:76	arg1	delivery					78:85	in vitro and oral delivery	60:85	in vitro and oral delivery of siRNA	60:94	Spheroplexes: Hybrid PLGA-cationic lipid nanoparticles, for in vitro and oral delivery of siRNA.					
35995297	5	1	theme	lipid	719:723	arg1	envelope					725:732	a lipid envelope	717:732	a lipid envelope adopting a lamellar structure, in which the siRNA is complexed, and they retain surface characteristics identical to the starting nanoparticles, i.e. lipoplexes siRNA	717:899	We elucidated their structure using several physico-chemical techniques and showed that they are composed of a hydrophobic PLGA matrix, surrounded by a lipid envelope adopting a lamellar structure, in which the siRNA is complexed, and they retain surface characteristics identical to the starting nanoparticles, i.e. lipoplexes siRNA.					
35995297	3	2	dep	lipoplexes	345:354	arg1	complexes					357:365	complexes	357:365	complexes of cationic lipid and siRNAs	357:394	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	3	from	poly	402:405	arg1	lipoplexes					345:354	lipoplexes	345:354	lipoplexes (complexes of cationic lipid and siRNAs)	345:395	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	8	4	theme	propria	1620:1626	arg1	level					1604:1608	the level	1600:1608	the level of lamina propria of the colon	1600:1639	We administered spheroplexes contain siRNAs targeting TNF-α to mice with ulcerative colitis induced by dextran sulfate and our results indicate a disease regression effect with a response probably mediated by their uptake by macrophages / monocytes at the level of lamina propria of the colon.					
35995297	5	5	theme	physico-chemical	611:626	arg1	techniques					628:637	several physico-chemical techniques	603:637	several physico-chemical techniques	603:637	We elucidated their structure using several physico-chemical techniques and showed that they are composed of a hydrophobic PLGA matrix, surrounded by a lipid envelope adopting a lamellar structure, in which the siRNA is complexed, and they retain surface characteristics identical to the starting nanoparticles, i.e. lipoplexes siRNA.					
35995297	9	6	theme	specific	1769:1776	arg1	siRNA					1778:1782	the specific siRNA	1765:1782	the specific siRNA	1765:1782	The efficacy of decreased level of TNF-α in vivo seemed to be an association of spheroplexes polymer-lipid composition and the specific siRNA.					
35995297	5	7	dep	siRNA	895:899	arg1	i.e.					879:882	i.e.	879:882	i.e.	879:882	We elucidated their structure using several physico-chemical techniques and showed that they are composed of a hydrophobic PLGA matrix, surrounded by a lipid envelope adopting a lamellar structure, in which the siRNA is complexed, and they retain surface characteristics identical to the starting nanoparticles, i.e. lipoplexes siRNA.					
35995297	8	8	theme	colon	1635:1639	arg1	propria					1620:1626	lamina propria	1613:1626	lamina propria of the colon	1613:1639	We administered spheroplexes contain siRNAs targeting TNF-α to mice with ulcerative colitis induced by dextran sulfate and our results indicate a disease regression effect with a response probably mediated by their uptake by macrophages / monocytes at the level of lamina propria of the colon.					
35995297	5	9	theme	several	603:609	arg1	techniques					628:637	several physico-chemical techniques	603:637	several physico-chemical techniques	603:637	We elucidated their structure using several physico-chemical techniques and showed that they are composed of a hydrophobic PLGA matrix, surrounded by a lipid envelope adopting a lamellar structure, in which the siRNA is complexed, and they retain surface characteristics identical to the starting nanoparticles, i.e. lipoplexes siRNA.					
35995297	5	10	theme	PLGA	690:693	arg1	matrix					695:700	a hydrophobic PLGA matrix	676:700	a hydrophobic PLGA matrix	676:700	We elucidated their structure using several physico-chemical techniques and showed that they are composed of a hydrophobic PLGA matrix, surrounded by a lipid envelope adopting a lamellar structure, in which the siRNA is complexed, and they retain surface characteristics identical to the starting nanoparticles, i.e. lipoplexes siRNA.					
35995297	8	11	dep	macrophages	1573:1583	arg1	monocytes					1587:1595	monocytes	1587:1595	macrophages / monocytes at the level of lamina propria of the colon	1573:1639	We administered spheroplexes contain siRNAs targeting TNF-α to mice with ulcerative colitis induced by dextran sulfate and our results indicate a disease regression effect with a response probably mediated by their uptake by macrophages / monocytes at the level of lamina propria of the colon.					
35995297	9	12	theme	composition	1749:1759	arg1	efficacy					1646:1653	The efficacy	1642:1653	The efficacy of decreased level of TNF-α in vivo	1642:1689	The efficacy of decreased level of TNF-α in vivo seemed to be an association of spheroplexes polymer-lipid composition and the specific siRNA.					
35995297	9	12	theme	composition	1749:1759	arg1	association					1707:1717	an association	1704:1717	an association of spheroplexes polymer-lipid composition and the specific siRNA	1704:1782	The efficacy of decreased level of TNF-α in vivo seemed to be an association of spheroplexes polymer-lipid composition and the specific siRNA.					
35995297	6	13	theme	flow	1129:1132	arg1	cytometry					1134:1142	flow cytometry	1129:1142	flow cytometry	1129:1142	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	7	14	theme	cultured	1332:1339	arg1	cells					1341:1345	cultured cells	1332:1345	cultured cells	1332:1345	Finally, we showed that spheroplexes are very stable particles and more efficient than siRNA lipoplexes for the delivery of siRNA to cultured cells.					
35995297	4	15	theme	hybrid	532:537	arg1	nanoparticles					539:551	these hybrid nanoparticles	526:551	these hybrid nanoparticles	526:551	Due to their morphology, we called these hybrid nanoparticles Spheroplexes.					
35995297	3	16	from	lipoplexes	345:354	arg1	objective					265:273	Our objective	261:273	Our objective	261:273	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	16	from	lipoplexes	345:354	arg1	nanoparticles					325:337	novel lipid-polymer hybrid nanoparticles	298:337	novel lipid-polymer hybrid nanoparticles	298:337	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	16	from	lipoplexes	345:354	arg1	poly					402:405	poly	402:405	poly (lactic-co-glycolic acid) (PLGA)	402:438	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	16	from	lipoplexes	345:354	arg1	PLGA					434:437	PLGA	434:437	PLGA	434:437	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	16	from	lipoplexes	345:354	arg1	development					283:293	the development	279:293	the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs),	279:396	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	16	from	lipoplexes	345:354	arg1	acid					427:430	lactic-co-glycolic acid	408:430	lactic-co-glycolic acid	408:430	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	10	17	theme	siRNA	1890:1894	arg1	delivery					1878:1885	the oral delivery	1869:1885	the oral delivery of siRNA to the colon	1869:1907	These results demonstrate that spheroplexes are a promising hybrid nanoparticle for the oral delivery of siRNA to the colon.					
35995297	3	18	theme	hybrid	318:323	arg1	nanoparticles					325:337	novel lipid-polymer hybrid nanoparticles	298:337	novel lipid-polymer hybrid nanoparticles	298:337	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	5	19	theme	starting	855:862	arg1	nanoparticles					864:876	the starting nanoparticles	851:876	the starting nanoparticles	851:876	We elucidated their structure using several physico-chemical techniques and showed that they are composed of a hydrophobic PLGA matrix, surrounded by a lipid envelope adopting a lamellar structure, in which the siRNA is complexed, and they retain surface characteristics identical to the starting nanoparticles, i.e. lipoplexes siRNA.					
35995297	3	20	theme	nanoprecipitation	465:481	arg1	method					483:488	a simple modified nanoprecipitation method	447:488	a simple modified nanoprecipitation method	447:488	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	7	21	theme	efficient	1271:1279	arg1	lipoplexes					1292:1301	more efficient than siRNA lipoplexes	1266:1301	more efficient than siRNA lipoplexes	1266:1301	Finally, we showed that spheroplexes are very stable particles and more efficient than siRNA lipoplexes for the delivery of siRNA to cultured cells.					
35995297	0	22	theme	siRNA	90:94	arg1	delivery					78:85	in vitro and oral delivery	60:85	in vitro and oral delivery of siRNA	60:94	Spheroplexes: Hybrid PLGA-cationic lipid nanoparticles, for in vitro and oral delivery of siRNA.					
35995297	9	23	theme	siRNA	1778:1782	arg1	efficacy					1646:1653	The efficacy	1642:1653	The efficacy of decreased level of TNF-α in vivo	1642:1689	The efficacy of decreased level of TNF-α in vivo seemed to be an association of spheroplexes polymer-lipid composition and the specific siRNA.					
35995297	9	23	theme	siRNA	1778:1782	arg1	association					1707:1717	an association	1704:1717	an association of spheroplexes polymer-lipid composition and the specific siRNA	1704:1782	The efficacy of decreased level of TNF-α in vivo seemed to be an association of spheroplexes polymer-lipid composition and the specific siRNA.					
35995297	6	24	theme	final	976:980	arg1	%					1041:1041	80 to 85%	1033:1041	80 to 85%	1033:1041	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	6	24	theme	final	976:980	arg1	percentage					982:991	the final percentage	972:991	the final percentage of spheroplexes within this population	972:1030	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	10	25	theme	promising	1835:1843	arg1	nanoparticle					1852:1863	a promising hybrid nanoparticle	1833:1863	a promising hybrid nanoparticle for the oral delivery of siRNA to the colon	1833:1907	These results demonstrate that spheroplexes are a promising hybrid nanoparticle for the oral delivery of siRNA to the colon.					
35995297	10	25	theme	promising	1835:1843	arg1	spheroplexes					1816:1827	spheroplexes	1816:1827	spheroplexes	1816:1827	These results demonstrate that spheroplexes are a promising hybrid nanoparticle for the oral delivery of siRNA to the colon.					
35995297	6	26	dep	particles	1165:1173	arg1	200 nm					1190:1195	200 nm	1190:1195	200 nm	1190:1195	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	9	27	theme	spheroplexes	1722:1733	arg1	composition					1749:1759	spheroplexes polymer-lipid composition	1722:1759	spheroplexes polymer-lipid composition	1722:1759	The efficacy of decreased level of TNF-α in vivo seemed to be an association of spheroplexes polymer-lipid composition and the specific siRNA.					
35995297	10	28	theme	oral	1873:1876	arg1	delivery					1878:1885	the oral delivery	1869:1885	the oral delivery of siRNA to the colon	1869:1907	These results demonstrate that spheroplexes are a promising hybrid nanoparticle for the oral delivery of siRNA to the colon.					
35995297	5	29	theme	lipoplexes	884:893	arg1	siRNA					895:899	lipoplexes siRNA	884:899	lipoplexes siRNA	884:899	We elucidated their structure using several physico-chemical techniques and showed that they are composed of a hydrophobic PLGA matrix, surrounded by a lipid envelope adopting a lamellar structure, in which the siRNA is complexed, and they retain surface characteristics identical to the starting nanoparticles, i.e. lipoplexes siRNA.					
35995297	9	30	theme	polymer-lipid	1735:1747	arg1	composition					1749:1759	spheroplexes polymer-lipid composition	1722:1759	spheroplexes polymer-lipid composition	1722:1759	The efficacy of decreased level of TNF-α in vivo seemed to be an association of spheroplexes polymer-lipid composition and the specific siRNA.					
35995297	8	31	theme	regression	1502:1511	arg1	effect					1513:1518	a disease regression effect	1492:1518	a disease regression effect	1492:1518	We administered spheroplexes contain siRNAs targeting TNF-α to mice with ulcerative colitis induced by dextran sulfate and our results indicate a disease regression effect with a response probably mediated by their uptake by macrophages / monocytes at the level of lamina propria of the colon.					
35995297	8	32	theme	ulcerative	1421:1430	arg1	colitis					1432:1438	ulcerative colitis	1421:1438	ulcerative colitis induced by dextran sulfate	1421:1465	We administered spheroplexes contain siRNAs targeting TNF-α to mice with ulcerative colitis induced by dextran sulfate and our results indicate a disease regression effect with a response probably mediated by their uptake by macrophages / monocytes at the level of lamina propria of the colon.					
35995297	5	33	theme	surface	814:820	arg1	characteristics					822:836	surface characteristics	814:836	surface characteristics	814:836	We elucidated their structure using several physico-chemical techniques and showed that they are composed of a hydrophobic PLGA matrix, surrounded by a lipid envelope adopting a lamellar structure, in which the siRNA is complexed, and they retain surface characteristics identical to the starting nanoparticles, i.e. lipoplexes siRNA.					
35995297	0	34	theme	PLGA-cationic	21:33	arg1	nanoparticles					41:53	Hybrid PLGA-cationic lipid nanoparticles	14:53	Hybrid PLGA-cationic lipid nanoparticles	14:53	Spheroplexes: Hybrid PLGA-cationic lipid nanoparticles, for in vitro and oral delivery of siRNA.					
35995297	1	35	used	used	151:154	arg2	siRNAs					132:137	siRNAs	132:137	siRNAs	132:137	Vectorized small interfering RNAs (siRNAs) are widely used to induce gene silencing.					
35995297	1	35	used	used	151:154	arg2	RNAs					126:129	Vectorized small interfering RNAs	97:129	Vectorized small interfering RNAs (siRNAs)	97:138	Vectorized small interfering RNAs (siRNAs) are widely used to induce gene silencing.					
35995297	8	36	theme	disease	1494:1500	arg1	effect					1513:1518	a disease regression effect	1492:1518	a disease regression effect	1492:1518	We administered spheroplexes contain siRNAs targeting TNF-α to mice with ulcerative colitis induced by dextran sulfate and our results indicate a disease regression effect with a response probably mediated by their uptake by macrophages / monocytes at the level of lamina propria of the colon.					
35995297	5	37	theme	lamellar	745:752	arg1	structure					754:762	a lamellar structure	743:762	a lamellar structure	743:762	We elucidated their structure using several physico-chemical techniques and showed that they are composed of a hydrophobic PLGA matrix, surrounded by a lipid envelope adopting a lamellar structure, in which the siRNA is complexed, and they retain surface characteristics identical to the starting nanoparticles, i.e. lipoplexes siRNA.					
35995297	7	38	theme	siRNA	1323:1327	arg1	delivery					1311:1318	the delivery	1307:1318	the delivery of siRNA to cultured cells	1307:1345	Finally, we showed that spheroplexes are very stable particles and more efficient than siRNA lipoplexes for the delivery of siRNA to cultured cells.					
35995297	1	39	theme	gene	166:169	arg1	silencing					171:179	gene silencing	166:179	gene silencing	166:179	Vectorized small interfering RNAs (siRNAs) are widely used to induce gene silencing.					
35995297	0	40	theme	Hybrid	14:19	arg1	nanoparticles					41:53	Hybrid PLGA-cationic lipid nanoparticles	14:53	Hybrid PLGA-cationic lipid nanoparticles	14:53	Spheroplexes: Hybrid PLGA-cationic lipid nanoparticles, for in vitro and oral delivery of siRNA.					
35995297	8	41	with	mice	1411:1414	arg1	colitis					1432:1438	ulcerative colitis	1421:1438	ulcerative colitis induced by dextran sulfate	1421:1465	We administered spheroplexes contain siRNAs targeting TNF-α to mice with ulcerative colitis induced by dextran sulfate and our results indicate a disease regression effect with a response probably mediated by their uptake by macrophages / monocytes at the level of lamina propria of the colon.					
35995297	8	42	from	level	1604:1608	arg1	monocytes					1587:1595	monocytes	1587:1595	macrophages / monocytes at the level of lamina propria of the colon	1573:1639	We administered spheroplexes contain siRNAs targeting TNF-α to mice with ulcerative colitis induced by dextran sulfate and our results indicate a disease regression effect with a response probably mediated by their uptake by macrophages / monocytes at the level of lamina propria of the colon.					
35995297	0	43	dep	in	60:61	arg1	vitro					63:67	vitro	63:67	vitro	63:67	Spheroplexes: Hybrid PLGA-cationic lipid nanoparticles, for in vitro and oral delivery of siRNA.					
35995297	8	44	dep	We	1348:1349	arg1	administered					1351:1362	administered	1351:1362	administered	1351:1362	We administered spheroplexes contain siRNAs targeting TNF-α to mice with ulcerative colitis induced by dextran sulfate and our results indicate a disease regression effect with a response probably mediated by their uptake by macrophages / monocytes at the level of lamina propria of the colon.					
35995297	5	45	theme	hydrophobic	678:688	arg1	matrix					695:700	a hydrophobic PLGA matrix	676:700	a hydrophobic PLGA matrix	676:700	We elucidated their structure using several physico-chemical techniques and showed that they are composed of a hydrophobic PLGA matrix, surrounded by a lipid envelope adopting a lamellar structure, in which the siRNA is complexed, and they retain surface characteristics identical to the starting nanoparticles, i.e. lipoplexes siRNA.					
35995297	6	46	theme	spheroplexes	996:1007	arg1	%					1041:1041	80 to 85%	1033:1041	80 to 85%	1033:1041	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	6	46	theme	spheroplexes	996:1007	arg1	percentage					982:991	the final percentage	972:991	the final percentage of spheroplexes within this population	972:1030	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	0	47	theme	lipid	35:39	arg1	nanoparticles					41:53	Hybrid PLGA-cationic lipid nanoparticles	14:53	Hybrid PLGA-cationic lipid nanoparticles	14:53	Spheroplexes: Hybrid PLGA-cationic lipid nanoparticles, for in vitro and oral delivery of siRNA.					
35995297	3	48	theme	siRNAs	389:394	arg1	complexes					357:365	complexes	357:365	complexes of cationic lipid and siRNAs	357:394	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	6	49	theme	population	946:955	arg1	composition					918:928	the composition	914:928	the composition of the particle population	914:955	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	6	50	dep	85	1039:1040	arg1	to					1036:1037	to	1036:1037	to	1036:1037	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	3	51	theme	nanoparticles	325:337	arg1	objective					265:273	Our objective	261:273	Our objective	261:273	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	51	theme	nanoparticles	325:337	arg1	development					283:293	the development	279:293	the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs),	279:396	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	51	theme	nanoparticles	325:337	arg1	poly					402:405	poly	402:405	poly (lactic-co-glycolic acid) (PLGA)	402:438	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	51	theme	nanoparticles	325:337	arg1	acid					427:430	lactic-co-glycolic acid	408:430	lactic-co-glycolic acid	408:430	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	51	theme	nanoparticles	325:337	arg1	PLGA					434:437	PLGA	434:437	PLGA	434:437	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	52	theme	cationic	370:377	arg1	lipid					379:383	cationic lipid	370:383	cationic lipid	370:383	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	9	53	theme	decreased	1658:1666	arg1	level					1668:1672	decreased level	1658:1672	decreased level of TNF-α in vivo	1658:1689	The efficacy of decreased level of TNF-α in vivo seemed to be an association of spheroplexes polymer-lipid composition and the specific siRNA.					
35995297	0	54	theme	in	60:61	arg1	delivery					78:85	in vitro and oral delivery	60:85	in vitro and oral delivery of siRNA	60:94	Spheroplexes: Hybrid PLGA-cationic lipid nanoparticles, for in vitro and oral delivery of siRNA.					
35995297	2	55	theme	delivery	192:199	arg1	systems					201:207	the delivery systems	188:207	the delivery systems used	188:212	Among the delivery systems used, lipid-based particles are the most effective.					
35995297	1	56	theme	Vectorized	97:106	arg1	siRNAs					132:137	siRNAs	132:137	siRNAs	132:137	Vectorized small interfering RNAs (siRNAs) are widely used to induce gene silencing.					
35995297	1	56	theme	Vectorized	97:106	arg1	RNAs					126:129	Vectorized small interfering RNAs	97:129	Vectorized small interfering RNAs (siRNAs)	97:138	Vectorized small interfering RNAs (siRNAs) are widely used to induce gene silencing.					
35995297	3	57	theme	lipid	379:383	arg1	complexes					357:365	complexes	357:365	complexes of cationic lipid and siRNAs	357:394	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	9	58	theme	level	1668:1672	arg1	efficacy					1646:1653	The efficacy	1642:1653	The efficacy of decreased level of TNF-α in vivo	1642:1689	The efficacy of decreased level of TNF-α in vivo seemed to be an association of spheroplexes polymer-lipid composition and the specific siRNA.					
35995297	9	58	theme	level	1668:1672	arg1	association					1707:1717	an association	1704:1717	an association of spheroplexes polymer-lipid composition and the specific siRNA	1704:1782	The efficacy of decreased level of TNF-α in vivo seemed to be an association of spheroplexes polymer-lipid composition and the specific siRNA.					
35995297	3	59	from	development	283:293	arg1	lipoplexes					345:354	lipoplexes	345:354	lipoplexes (complexes of cationic lipid and siRNAs)	345:395	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	10	60	theme	hybrid	1845:1850	arg1	nanoparticle					1852:1863	a promising hybrid nanoparticle	1833:1863	a promising hybrid nanoparticle for the oral delivery of siRNA to the colon	1833:1907	These results demonstrate that spheroplexes are a promising hybrid nanoparticle for the oral delivery of siRNA to the colon.					
35995297	10	60	theme	hybrid	1845:1850	arg1	spheroplexes					1816:1827	spheroplexes	1816:1827	spheroplexes	1816:1827	These results demonstrate that spheroplexes are a promising hybrid nanoparticle for the oral delivery of siRNA to the colon.					
35995297	1	61	theme	small	108:112	arg1	siRNAs					132:137	siRNAs	132:137	siRNAs	132:137	Vectorized small interfering RNAs (siRNAs) are widely used to induce gene silencing.					
35995297	1	61	theme	small	108:112	arg1	RNAs					126:129	Vectorized small interfering RNAs	97:129	Vectorized small interfering RNAs (siRNAs)	97:138	Vectorized small interfering RNAs (siRNAs) are widely used to induce gene silencing.					
35995297	6	62	theme	cytometry	1134:1142	arg1	markers					1102:1108	fluorescent markers	1090:1108	fluorescent markers	1090:1108	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	6	62	theme	cytometry	1134:1142	arg1	ability					1118:1124	the ability	1114:1124	the ability of flow cytometry to detect nanometric particles (approximately 200 nm)	1114:1196	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	6	63	theme	nanometric	1154:1163	arg1	particles					1165:1173	nanometric particles	1154:1173	nanometric particles (approximately 200 nm)	1154:1196	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	1	64	theme	interfering	114:124	arg1	siRNAs					132:137	siRNAs	132:137	siRNAs	132:137	Vectorized small interfering RNAs (siRNAs) are widely used to induce gene silencing.					
35995297	1	64	theme	interfering	114:124	arg1	RNAs					126:129	Vectorized small interfering RNAs	97:129	Vectorized small interfering RNAs (siRNAs)	97:138	Vectorized small interfering RNAs (siRNAs) are widely used to induce gene silencing.					
35995297	6	65	theme	fluorescent	1090:1100	arg1	markers					1102:1108	fluorescent markers	1090:1108	fluorescent markers	1090:1108	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	6	66	theme	particle	937:944	arg1	population					946:955	the particle population	933:955	the particle population	933:955	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	9	67	theme	TNF-α	1677:1681	arg1	level					1668:1672	decreased level	1658:1672	decreased level of TNF-α in vivo	1658:1689	The efficacy of decreased level of TNF-α in vivo seemed to be an association of spheroplexes polymer-lipid composition and the specific siRNA.					
35995297	5	68	theme	identical	838:846	arg1	characteristics					822:836	surface characteristics	814:836	surface characteristics	814:836	We elucidated their structure using several physico-chemical techniques and showed that they are composed of a hydrophobic PLGA matrix, surrounded by a lipid envelope adopting a lamellar structure, in which the siRNA is complexed, and they retain surface characteristics identical to the starting nanoparticles, i.e. lipoplexes siRNA.					
35995297	8	69	theme	lamina	1613:1618	arg1	propria					1620:1626	lamina propria	1613:1626	lamina propria of the colon	1613:1639	We administered spheroplexes contain siRNAs targeting TNF-α to mice with ulcerative colitis induced by dextran sulfate and our results indicate a disease regression effect with a response probably mediated by their uptake by macrophages / monocytes at the level of lamina propria of the colon.					
35995297	6	70	theme	preparation	1060:1070	arg1	conditions					1072:1081	the preparation conditions	1056:1081	the preparation conditions	1056:1081	We analyzed the composition of the particle population and determined the final percentage of spheroplexes within this population, 80 to 85% depending on the preparation conditions, using fluorescent markers and the ability of flow cytometry to detect nanometric particles (approximately 200 nm).					
35995297	3	71	theme	novel	298:302	arg1	nanoparticles					325:337	novel lipid-polymer hybrid nanoparticles	298:337	novel lipid-polymer hybrid nanoparticles	298:337	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	72	theme	lactic-co-glycolic	408:425	arg1	poly					402:405	poly	402:405	poly (lactic-co-glycolic acid) (PLGA)	402:438	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	72	theme	lactic-co-glycolic	408:425	arg1	acid					427:430	lactic-co-glycolic acid	408:430	lactic-co-glycolic acid	408:430	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	2	73	theme	lipid-based	215:225	arg1	effective					250:258	effective	250:258	effective	250:258	Among the delivery systems used, lipid-based particles are the most effective.					
35995297	2	73	theme	lipid-based	215:225	arg1	particles					227:235	lipid-based particles	215:235	lipid-based particles	215:235	Among the delivery systems used, lipid-based particles are the most effective.					
35995297	7	74	theme	stable	1245:1250	arg1	particles					1252:1260	very stable particles	1240:1260	very stable particles	1240:1260	Finally, we showed that spheroplexes are very stable particles and more efficient than siRNA lipoplexes for the delivery of siRNA to cultured cells.					
35995297	7	74	theme	stable	1245:1250	arg1	spheroplexes					1223:1234	spheroplexes	1223:1234	spheroplexes	1223:1234	Finally, we showed that spheroplexes are very stable particles and more efficient than siRNA lipoplexes for the delivery of siRNA to cultured cells.					
35995297	3	75	theme	simple	449:454	arg1	method					483:488	a simple modified nanoprecipitation method	447:488	a simple modified nanoprecipitation method	447:488	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	3	76	theme	lipid-polymer	304:316	arg1	nanoparticles					325:337	novel lipid-polymer hybrid nanoparticles	298:337	novel lipid-polymer hybrid nanoparticles	298:337	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35995297	8	77	theme	dextran	1451:1457	arg1	sulfate					1459:1465	dextran sulfate	1451:1465	dextran sulfate	1451:1465	We administered spheroplexes contain siRNAs targeting TNF-α to mice with ulcerative colitis induced by dextran sulfate and our results indicate a disease regression effect with a response probably mediated by their uptake by macrophages / monocytes at the level of lamina propria of the colon.					
35995297	0	78	dep	Spheroplexes	0:11	arg1	nanoparticles					41:53	Hybrid PLGA-cationic lipid nanoparticles	14:53	Hybrid PLGA-cationic lipid nanoparticles	14:53	Spheroplexes: Hybrid PLGA-cationic lipid nanoparticles, for in vitro and oral delivery of siRNA.					
35995297	3	79	theme	modified	456:463	arg1	method					483:488	a simple modified nanoprecipitation method	447:488	a simple modified nanoprecipitation method	447:488	Our objective was the development of novel lipid-polymer hybrid nanoparticles, from lipoplexes (complexes of cationic lipid and siRNAs), and poly (lactic-co-glycolic acid) (PLGA), using a simple modified nanoprecipitation method.					
35667460	0	0	theme	insulin	80:86	arg1	sensitivity					88:98	insulin sensitivity	80:98	insulin sensitivity	80:98	Chondroitin sulfate stimulates the secretion of H2S by Desulfovibrio to improve insulin sensitivity in NAFLD mice.					
35667460	4	1	theme	insulin	589:595	arg1	resistance					597:606	diet-induced insulin resistance	576:606	diet-induced insulin resistance	576:606	CS could ameliorate diet-induced insulin resistance and improve insulin sensitivity via the AKT pathway, and modulate gut microbiota composition, especially increased the abundance of Desulfovibrio and elevated levels of hydrogen sulfide (H2S).					
35667460	3	2	theme	body	479:482	arg1	gain					491:494	body weight gain	479:494	body weight gain	479:494	A 7-week CS supplementation had beneficial effects on body weight gain, liver function, hepatic histology, and serum lipid levels.					
35667460	5	3	theme	metabolic	923:931	arg1	H2S					933:935	the metabolic H2S	919:935	the metabolic H2S	919:935	Collectively, these findings suggested that CS treatment was positively correlated with Desulfovibrio in the gut, and the metabolic H2S flowed into the liver via the gut-liver axis, thereby triggering the AKT signaling pathway and improving insulin resistance.					
35667460	6	4	theme	gut	1098:1100	arg1	microbiota					1102:1111	the gut microbiota	1094:1111	the gut microbiota	1094:1111	Thus, CS-induced alterations in the gut microbiota seem a promising for ameliorating NAFLD.					
35667460	4	5	dep	composition	689:699	arg1	increased					713:721	increased	713:721	increased the abundance of Desulfovibrio	713:752	CS could ameliorate diet-induced insulin resistance and improve insulin sensitivity via the AKT pathway, and modulate gut microbiota composition, especially increased the abundance of Desulfovibrio and elevated levels of hydrogen sulfide (H2S).					
35667460	4	5	dep	composition	689:699	arg1	elevated					758:765	elevated	758:765	elevated levels of hydrogen sulfide (H2S)	758:798	CS could ameliorate diet-induced insulin resistance and improve insulin sensitivity via the AKT pathway, and modulate gut microbiota composition, especially increased the abundance of Desulfovibrio and elevated levels of hydrogen sulfide (H2S).					
35667460	3	6	contain	had	453:455	arg1	supplementation					437:451	A 7-week CS supplementation	425:451	A 7-week CS supplementation	425:451	A 7-week CS supplementation had beneficial effects on body weight gain, liver function, hepatic histology, and serum lipid levels.					
35667460	3	6	contain	had	453:455	arg2	effects					468:474	beneficial effects	457:474	beneficial effects	457:474	A 7-week CS supplementation had beneficial effects on body weight gain, liver function, hepatic histology, and serum lipid levels.					
35667460	4	7	theme	microbiota	678:687	arg1	composition					689:699	gut microbiota composition	674:699	gut microbiota composition	674:699	CS could ameliorate diet-induced insulin resistance and improve insulin sensitivity via the AKT pathway, and modulate gut microbiota composition, especially increased the abundance of Desulfovibrio and elevated levels of hydrogen sulfide (H2S).					
35667460	4	8	theme	gut	674:676	arg1	composition					689:699	gut microbiota composition	674:699	gut microbiota composition	674:699	CS could ameliorate diet-induced insulin resistance and improve insulin sensitivity via the AKT pathway, and modulate gut microbiota composition, especially increased the abundance of Desulfovibrio and elevated levels of hydrogen sulfide (H2S).					
35667460	3	9	theme	CS	434:435	arg1	supplementation					437:451	A 7-week CS supplementation	425:451	A 7-week CS supplementation	425:451	A 7-week CS supplementation had beneficial effects on body weight gain, liver function, hepatic histology, and serum lipid levels.					
35667460	2	10	theme	present	257:263	arg1	study					265:269	The present study	253:269	The present study	253:269	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	1	11	theme	bioactive	143:151	arg1	sulfide					124:130	Hydrogen sulfide	115:130	Hydrogen sulfide (H2S)	115:136	Hydrogen sulfide (H2S) is a bioactive gas regulating insulin secretion and sensitivity, produced by sulfate-reducing bacteria in the gut.					
35667460	1	11	theme	bioactive	143:151	arg1	gas					153:155	a bioactive gas	141:155	a bioactive gas	141:155	Hydrogen sulfide (H2S) is a bioactive gas regulating insulin secretion and sensitivity, produced by sulfate-reducing bacteria in the gut.					
35667460	0	12	theme	NAFLD	103:107	arg1	mice					109:112	NAFLD mice	103:112	NAFLD mice	103:112	Chondroitin sulfate stimulates the secretion of H2S by Desulfovibrio to improve insulin sensitivity in NAFLD mice.					
35667460	3	13	theme	liver	497:501	arg1	function					503:510	liver function	497:510	liver function	497:510	A 7-week CS supplementation had beneficial effects on body weight gain, liver function, hepatic histology, and serum lipid levels.					
35667460	2	14	theme	H2S	365:367	arg1	production					369:378	the H2S production	361:378	the H2S production on nonalcoholic fatty liver disease (NAFLD)	361:422	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	2	15	theme	chondroitin	298:308	arg1	CS					319:320	CS	319:320	CS	319:320	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	2	15	theme	chondroitin	298:308	arg1	sulfate					310:316	chondroitin sulfate	298:316	chondroitin sulfate (CS) treatment	298:331	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	0	16	theme	Chondroitin	0:10	arg1	sulfate					12:18	Chondroitin sulfate	0:18	Chondroitin sulfate	0:18	Chondroitin sulfate stimulates the secretion of H2S by Desulfovibrio to improve insulin sensitivity in NAFLD mice.					
35667460	1	17	theme	insulin	168:174	arg1	secretion					176:184	insulin secretion	168:184	insulin secretion	168:184	Hydrogen sulfide (H2S) is a bioactive gas regulating insulin secretion and sensitivity, produced by sulfate-reducing bacteria in the gut.					
35667460	5	18	from	Desulfovibrio	889:901	arg1	gut					910:912	the gut	906:912	the gut	906:912	Collectively, these findings suggested that CS treatment was positively correlated with Desulfovibrio in the gut, and the metabolic H2S flowed into the liver via the gut-liver axis, thereby triggering the AKT signaling pathway and improving insulin resistance.					
35667460	3	19	theme	hepatic	513:519	arg1	histology					521:529	hepatic histology	513:529	hepatic histology	513:529	A 7-week CS supplementation had beneficial effects on body weight gain, liver function, hepatic histology, and serum lipid levels.					
35667460	2	20	theme	liver	402:406	arg1	NAFLD					417:421	NAFLD	417:421	NAFLD	417:421	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	2	20	theme	liver	402:406	arg1	disease					408:414	nonalcoholic fatty liver disease	383:414	nonalcoholic fatty liver disease (NAFLD)	383:422	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	6	21	theme	CS-induced	1068:1077	arg1	alterations					1079:1089	CS-induced alterations	1068:1089	CS-induced alterations in the gut microbiota	1068:1111	Thus, CS-induced alterations in the gut microbiota seem a promising for ameliorating NAFLD.					
35667460	2	22	theme	treatment	323:331	arg1	effect					288:293	the effect	284:293	the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD)	284:422	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	2	23	theme	fatty	396:400	arg1	NAFLD					417:421	NAFLD	417:421	NAFLD	417:421	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	2	23	theme	fatty	396:400	arg1	disease					408:414	nonalcoholic fatty liver disease	383:414	nonalcoholic fatty liver disease (NAFLD)	383:422	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	4	24	theme	diet-induced	576:587	arg1	resistance					597:606	diet-induced insulin resistance	576:606	diet-induced insulin resistance	576:606	CS could ameliorate diet-induced insulin resistance and improve insulin sensitivity via the AKT pathway, and modulate gut microbiota composition, especially increased the abundance of Desulfovibrio and elevated levels of hydrogen sulfide (H2S).					
35667460	5	25	theme	gut-liver	967:975	arg1	axis					977:980	the gut-liver axis	963:980	the gut-liver axis	963:980	Collectively, these findings suggested that CS treatment was positively correlated with Desulfovibrio in the gut, and the metabolic H2S flowed into the liver via the gut-liver axis, thereby triggering the AKT signaling pathway and improving insulin resistance.					
35667460	5	26	theme	CS	845:846	arg1	treatment					848:856	CS treatment	845:856	CS treatment	845:856	Collectively, these findings suggested that CS treatment was positively correlated with Desulfovibrio in the gut, and the metabolic H2S flowed into the liver via the gut-liver axis, thereby triggering the AKT signaling pathway and improving insulin resistance.					
35667460	2	27	theme	nonalcoholic	383:394	arg1	NAFLD					417:421	NAFLD	417:421	NAFLD	417:421	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	2	27	theme	nonalcoholic	383:394	arg1	disease					408:414	nonalcoholic fatty liver disease	383:414	nonalcoholic fatty liver disease (NAFLD)	383:422	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	4	28	theme	sulfide	786:792	arg1	levels					767:772	levels	767:772	levels of hydrogen sulfide (H2S)	767:798	CS could ameliorate diet-induced insulin resistance and improve insulin sensitivity via the AKT pathway, and modulate gut microbiota composition, especially increased the abundance of Desulfovibrio and elevated levels of hydrogen sulfide (H2S).					
35667460	6	29	from	alterations	1079:1089	arg1	microbiota					1102:1111	the gut microbiota	1094:1111	the gut microbiota	1094:1111	Thus, CS-induced alterations in the gut microbiota seem a promising for ameliorating NAFLD.					
35667460	5	30	theme	insulin	1042:1048	arg1	resistance					1050:1059	insulin resistance	1042:1059	insulin resistance	1042:1059	Collectively, these findings suggested that CS treatment was positively correlated with Desulfovibrio in the gut, and the metabolic H2S flowed into the liver via the gut-liver axis, thereby triggering the AKT signaling pathway and improving insulin resistance.					
35667460	3	31	theme	serum	536:540	arg1	levels					548:553	serum lipid levels	536:553	serum lipid levels	536:553	A 7-week CS supplementation had beneficial effects on body weight gain, liver function, hepatic histology, and serum lipid levels.					
35667460	3	32	theme	beneficial	457:466	arg1	effects					468:474	beneficial effects	457:474	beneficial effects	457:474	A 7-week CS supplementation had beneficial effects on body weight gain, liver function, hepatic histology, and serum lipid levels.					
35667460	0	33	theme	H2S	48:50	arg1	secretion					35:43	the secretion	31:43	the secretion of H2S by Desulfovibrio	31:67	Chondroitin sulfate stimulates the secretion of H2S by Desulfovibrio to improve insulin sensitivity in NAFLD mice.					
35667460	1	34	from	bacteria	232:239	arg1	gut					248:250	the gut	244:250	the gut	244:250	Hydrogen sulfide (H2S) is a bioactive gas regulating insulin secretion and sensitivity, produced by sulfate-reducing bacteria in the gut.					
35667460	3	35	theme	lipid	542:546	arg1	levels					548:553	serum lipid levels	536:553	serum lipid levels	536:553	A 7-week CS supplementation had beneficial effects on body weight gain, liver function, hepatic histology, and serum lipid levels.					
35667460	2	36	from	production	369:378	arg1	NAFLD					417:421	NAFLD	417:421	NAFLD	417:421	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	2	36	from	production	369:378	arg1	disease					408:414	nonalcoholic fatty liver disease	383:414	nonalcoholic fatty liver disease (NAFLD)	383:422	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	1	37	theme	Hydrogen	115:122	arg1	sulfide					124:130	Hydrogen sulfide	115:130	Hydrogen sulfide (H2S)	115:136	Hydrogen sulfide (H2S) is a bioactive gas regulating insulin secretion and sensitivity, produced by sulfate-reducing bacteria in the gut.					
35667460	1	37	theme	Hydrogen	115:122	arg1	gas					153:155	a bioactive gas	141:155	a bioactive gas	141:155	Hydrogen sulfide (H2S) is a bioactive gas regulating insulin secretion and sensitivity, produced by sulfate-reducing bacteria in the gut.					
35667460	1	37	theme	Hydrogen	115:122	arg1	H2S					133:135	H2S	133:135	H2S	133:135	Hydrogen sulfide (H2S) is a bioactive gas regulating insulin secretion and sensitivity, produced by sulfate-reducing bacteria in the gut.					
35667460	4	38	theme	Desulfovibrio	740:752	arg1	abundance					727:735	the abundance	723:735	the abundance of Desulfovibrio	723:752	CS could ameliorate diet-induced insulin resistance and improve insulin sensitivity via the AKT pathway, and modulate gut microbiota composition, especially increased the abundance of Desulfovibrio and elevated levels of hydrogen sulfide (H2S).					
35667460	5	39	theme	AKT	1006:1008	arg1	pathway					1020:1026	the AKT signaling pathway	1002:1026	the AKT signaling pathway	1002:1026	Collectively, these findings suggested that CS treatment was positively correlated with Desulfovibrio in the gut, and the metabolic H2S flowed into the liver via the gut-liver axis, thereby triggering the AKT signaling pathway and improving insulin resistance.					
35667460	2	40	theme	sulfate	310:316	arg1	treatment					323:331	chondroitin sulfate (CS) treatment	298:331	chondroitin sulfate (CS) treatment	298:331	The present study investigated the effect of chondroitin sulfate (CS) treatment, which indirectly increased the H2S production on nonalcoholic fatty liver disease (NAFLD).					
35667460	5	41	theme	signaling	1010:1018	arg1	pathway					1020:1026	the AKT signaling pathway	1002:1026	the AKT signaling pathway	1002:1026	Collectively, these findings suggested that CS treatment was positively correlated with Desulfovibrio in the gut, and the metabolic H2S flowed into the liver via the gut-liver axis, thereby triggering the AKT signaling pathway and improving insulin resistance.					
35667460	4	42	theme	hydrogen	777:784	arg1	H2S					795:797	H2S	795:797	H2S	795:797	CS could ameliorate diet-induced insulin resistance and improve insulin sensitivity via the AKT pathway, and modulate gut microbiota composition, especially increased the abundance of Desulfovibrio and elevated levels of hydrogen sulfide (H2S).					
35667460	4	42	theme	hydrogen	777:784	arg1	sulfide					786:792	hydrogen sulfide	777:792	hydrogen sulfide (H2S)	777:798	CS could ameliorate diet-induced insulin resistance and improve insulin sensitivity via the AKT pathway, and modulate gut microbiota composition, especially increased the abundance of Desulfovibrio and elevated levels of hydrogen sulfide (H2S).					
35667460	3	43	theme	7-week	427:432	arg1	supplementation					437:451	A 7-week CS supplementation	425:451	A 7-week CS supplementation	425:451	A 7-week CS supplementation had beneficial effects on body weight gain, liver function, hepatic histology, and serum lipid levels.					
35667460	3	44	theme	weight	484:489	arg1	gain					491:494	body weight gain	479:494	body weight gain	479:494	A 7-week CS supplementation had beneficial effects on body weight gain, liver function, hepatic histology, and serum lipid levels.					
35667460	4	45	theme	insulin	620:626	arg1	sensitivity					628:638	insulin sensitivity	620:638	insulin sensitivity	620:638	CS could ameliorate diet-induced insulin resistance and improve insulin sensitivity via the AKT pathway, and modulate gut microbiota composition, especially increased the abundance of Desulfovibrio and elevated levels of hydrogen sulfide (H2S).					
35667460	1	46	theme	sulfate-reducing	215:230	arg1	bacteria					232:239	sulfate-reducing bacteria	215:239	sulfate-reducing bacteria in the gut	215:250	Hydrogen sulfide (H2S) is a bioactive gas regulating insulin secretion and sensitivity, produced by sulfate-reducing bacteria in the gut.					
35667460	4	47	theme	AKT	648:650	arg1	pathway					652:658	the AKT pathway	644:658	the AKT pathway	644:658	CS could ameliorate diet-induced insulin resistance and improve insulin sensitivity via the AKT pathway, and modulate gut microbiota composition, especially increased the abundance of Desulfovibrio and elevated levels of hydrogen sulfide (H2S).					
36374637	0	0	theme	mulch	104:108	arg1	films					110:114	agricultural mulch films	91:114	agricultural mulch films for green farming	91:132	All-biodegradable soy protein isolate/lignin composite cross-linked by oxidized sucrose as agricultural mulch films for green farming.					
36374637	4	1	theme	SPI/OS/Lignin	947:959	arg1	film					961:964	SPI/OS/Lignin film	947:964	SPI/OS/Lignin film	947:964	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	1	2	theme	traditional	177:187	arg1	film					210:213	the discarded traditional non-degradable mulch film	163:213	the discarded traditional non-degradable mulch film	163:213	Microplastics produced from the discarded traditional non-degradable mulch film deteriorate the ecological environment and pose a great threat to human health.					
36374637	1	3	theme	human	281:285	arg1	health					287:292	human health	281:292	human health	281:292	Microplastics produced from the discarded traditional non-degradable mulch film deteriorate the ecological environment and pose a great threat to human health.					
36374637	0	4	theme	agricultural	91:102	arg1	films					110:114	agricultural mulch films	91:114	agricultural mulch films for green farming	91:132	All-biodegradable soy protein isolate/lignin composite cross-linked by oxidized sucrose as agricultural mulch films for green farming.					
36374637	6	5	theme	seed	1209:1212	arg1	rate					1193:1196	higher germination rate	1174:1196	higher germination rate of cabbage seed	1174:1212	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	1	6	theme	non-degradable	189:202	arg1	film					210:213	the discarded traditional non-degradable mulch film	163:213	the discarded traditional non-degradable mulch film	163:213	Microplastics produced from the discarded traditional non-degradable mulch film deteriorate the ecological environment and pose a great threat to human health.					
36374637	6	7	theme	cabbage	1201:1207	arg1	seed					1209:1212	cabbage seed	1201:1212	cabbage seed	1201:1212	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	6	8	theme	traditional	1308:1318	arg1	film					1335:1338	traditional low-density PE film	1308:1338	traditional low-density PE film	1308:1338	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	7	9	theme	crops	1484:1488	arg1	growth					1490:1495	the crops growth	1480:1495	the crops growth	1480:1495	Our findings paved a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth, boosting the development of green farming.					
36374637	4	10	theme	pristine	914:921	arg1	film					927:930	pristine SPI film	914:930	pristine SPI film	914:930	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	11	theme	intermolecular	841:854	arg1	bonds					781:785	sacrificial bonds	769:785	sacrificial bonds for energy dissipation	769:808	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	11	theme	intermolecular	841:854	arg1	strength					888:895	tensile strength	880:895	the intermolecular interactions as well as tensile strength	837:895	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	11	theme	intermolecular	841:854	arg1	interactions					856:867	the intermolecular interactions	837:867	the intermolecular interactions as well as tensile strength	837:895	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	11	theme	intermolecular	841:854	arg1	effect					716:721	The cross-linking effect and hydrogen bonds	698:740	effect	716:721	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	11	theme	intermolecular	841:854	arg1	bonds					736:740	The cross-linking effect and hydrogen bonds	698:740	bonds	736:740	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	5	12	theme	excellent	1021:1029	arg1	properties					1117:1126	excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties	1021:1126	excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties	1021:1126	Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties.					
36374637	4	13	theme	film	961:964	arg1	8.45 MPa					935:942	8.45 MPa	935:942	8.45 MPa of SPI/OS/Lignin film	935:964	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	3	14	theme	OS	550:551	arg1	film					567:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	3	15	theme	/oxidized	531:539	arg1	film					567:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	5	16	theme	mulch	995:999	arg1	film					1001:1004	the SPI/OS/Lignin mulch film	977:1004	the SPI/OS/Lignin mulch film	977:1004	Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties.					
36374637	4	17	theme	film	927:930	arg1	6.67 MPa					902:909	6.67 MPa	902:909	6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film	902:964	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	6	18	theme	above-mentioned	1150:1164	arg1	merits					1166:1171	the above-mentioned merits	1146:1171	the above-mentioned merits	1146:1171	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	3	19	theme	film	567:570	arg1	category					459:466	a category	457:466	a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	457:570	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	3	20	theme	eco-friendly	471:482	arg1	film					567:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	1	21	theme	mulch	204:208	arg1	film					210:213	the discarded traditional non-degradable mulch film	163:213	the discarded traditional non-degradable mulch film	163:213	Microplastics produced from the discarded traditional non-degradable mulch film deteriorate the ecological environment and pose a great threat to human health.					
36374637	0	22	theme	green	120:124	arg1	farming					126:132	green farming	120:132	green farming	120:132	All-biodegradable soy protein isolate/lignin composite cross-linked by oxidized sucrose as agricultural mulch films for green farming.					
36374637	5	23	theme	SPI/OS/Lignin	981:993	arg1	film					1001:1004	the SPI/OS/Lignin mulch film	977:1004	the SPI/OS/Lignin mulch film	977:1004	Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties.					
36374637	4	24	theme	SPI	923:925	arg1	film					927:930	pristine SPI film	914:930	pristine SPI film	914:930	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	7	25	from	way	1368:1370	arg1	fabricating					1398:1408	fabricating	1398:1408	fabricating	1398:1408	Our findings paved a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth, boosting the development of green farming.					
36374637	7	25	from	way	1368:1370	arg1	designing					1384:1392	designing	1384:1392	designing	1384:1392	Our findings paved a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth, boosting the development of green farming.					
36374637	5	26	theme	preservation	1070:1081	arg1	effect					1083:1088	heat preservation effect	1065:1088	heat preservation effect	1065:1088	Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties.					
36374637	3	27	theme	soy	506:508	arg1	film					567:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	0	28	theme	soy	18:20	arg1	composite					45:53	All-biodegradable soy protein isolate/lignin composite	0:53	All-biodegradable soy protein isolate/lignin composite	0:53	All-biodegradable soy protein isolate/lignin composite cross-linked by oxidized sucrose as agricultural mulch films for green farming.					
36374637	3	29	theme	mulch	561:565	arg1	film					567:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	7	30	theme	solid	1362:1366	arg1	way					1368:1370	a solid way	1360:1370	a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth	1360:1495	Our findings paved a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth, boosting the development of green farming.					
36374637	2	31	theme	plastic	373:379	arg1	film					387:390	traditional plastic mulch film	361:390	traditional plastic mulch film highly contributed to the progress of green agriculture	361:446	Developing eco-friendly and biodegradable materials to substitute traditional plastic mulch film highly contributed to the progress of green agriculture.					
36374637	6	32	theme	higher	1174:1179	arg1	rate					1193:1196	higher germination rate	1174:1196	higher germination rate of cabbage seed	1174:1212	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	2	33	theme	agriculture	436:446	arg1	progress					418:425	the progress	414:425	the progress of green agriculture	414:446	Developing eco-friendly and biodegradable materials to substitute traditional plastic mulch film highly contributed to the progress of green agriculture.					
36374637	0	34	theme	All-biodegradable	0:16	arg1	composite					45:53	All-biodegradable soy protein isolate/lignin composite	0:53	All-biodegradable soy protein isolate/lignin composite	0:53	All-biodegradable soy protein isolate/lignin composite cross-linked by oxidized sucrose as agricultural mulch films for green farming.					
36374637	7	35	theme	low-cost	1424:1431	arg1	film					1461:1464	eco-friendly, low-cost and all-biodegradable mulch film	1410:1464	eco-friendly, low-cost and all-biodegradable mulch film	1410:1464	Our findings paved a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth, boosting the development of green farming.					
36374637	6	36	from	merits	1166:1171	arg1	Benefitting					1129:1139	Benefitting	1129:1139	Benefitting	1129:1139	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	2	37	theme	traditional	361:371	arg1	film					387:390	traditional plastic mulch film	361:390	traditional plastic mulch film highly contributed to the progress of green agriculture	361:446	Developing eco-friendly and biodegradable materials to substitute traditional plastic mulch film highly contributed to the progress of green agriculture.					
36374637	2	38	theme	green	430:434	arg1	agriculture					436:446	green agriculture	430:446	green agriculture	430:446	Developing eco-friendly and biodegradable materials to substitute traditional plastic mulch film highly contributed to the progress of green agriculture.					
36374637	0	39	theme	isolate/lignin	30:43	arg1	composite					45:53	All-biodegradable soy protein isolate/lignin composite	0:53	All-biodegradable soy protein isolate/lignin composite	0:53	All-biodegradable soy protein isolate/lignin composite cross-linked by oxidized sucrose as agricultural mulch films for green farming.					
36374637	7	40	theme	green	1526:1530	arg1	farming					1532:1538	green farming	1526:1538	green farming	1526:1538	Our findings paved a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth, boosting the development of green farming.					
36374637	6	41	theme	PE	1332:1333	arg1	film					1335:1338	traditional low-density PE film	1308:1338	traditional low-density PE film	1308:1338	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	1	42	theme	ecological	231:240	arg1	environment					242:252	the ecological environment	227:252	the ecological environment	227:252	Microplastics produced from the discarded traditional non-degradable mulch film deteriorate the ecological environment and pose a great threat to human health.					
36374637	0	43	theme	protein	22:28	arg1	composite					45:53	All-biodegradable soy protein isolate/lignin composite	0:53	All-biodegradable soy protein isolate/lignin composite	0:53	All-biodegradable soy protein isolate/lignin composite cross-linked by oxidized sucrose as agricultural mulch films for green farming.					
36374637	5	44	theme	UV-shielding	1031:1042	arg1	properties					1117:1126	excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties	1021:1126	excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties	1021:1126	Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties.					
36374637	5	45	theme	moisture	1045:1052	arg1	retention					1054:1062	moisture retention	1045:1062	moisture retention	1045:1062	Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties.					
36374637	3	46	theme	SPI	527:529	arg1	film					567:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	5	47	theme	retention	1054:1062	arg1	properties					1117:1126	excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties	1021:1126	excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties	1021:1126	Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties.					
36374637	4	48	theme	cross-linking	702:714	arg1	bonds					781:785	sacrificial bonds	769:785	sacrificial bonds for energy dissipation	769:808	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	48	theme	cross-linking	702:714	arg1	strength					888:895	tensile strength	880:895	the intermolecular interactions as well as tensile strength	837:895	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	48	theme	cross-linking	702:714	arg1	interactions					856:867	the intermolecular interactions	837:867	the intermolecular interactions as well as tensile strength	837:895	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	48	theme	cross-linking	702:714	arg1	effect					716:721	The cross-linking effect and hydrogen bonds	698:740	effect	716:721	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	48	theme	cross-linking	702:714	arg1	bonds					736:740	The cross-linking effect and hydrogen bonds	698:740	bonds	736:740	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	5	49	theme	release	1109:1115	arg1	properties					1117:1126	excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties	1021:1126	excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties	1021:1126	Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties.					
36374637	4	50	theme	hydrogen	727:734	arg1	bonds					781:785	sacrificial bonds	769:785	sacrificial bonds for energy dissipation	769:808	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	50	theme	hydrogen	727:734	arg1	strength					888:895	tensile strength	880:895	the intermolecular interactions as well as tensile strength	837:895	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	50	theme	hydrogen	727:734	arg1	interactions					856:867	the intermolecular interactions	837:867	the intermolecular interactions as well as tensile strength	837:895	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	50	theme	hydrogen	727:734	arg1	effect					716:721	The cross-linking effect and hydrogen bonds	698:740	effect	716:721	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	50	theme	hydrogen	727:734	arg1	bonds					736:740	The cross-linking effect and hydrogen bonds	698:740	bonds	736:740	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	3	51	theme	protein	510:516	arg1	film					567:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	3	52	theme	all-biodegradable	488:504	arg1	film					567:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	7	53	theme	all-biodegradable	1437:1453	arg1	film					1461:1464	eco-friendly, low-cost and all-biodegradable mulch film	1410:1464	eco-friendly, low-cost and all-biodegradable mulch film	1410:1464	Our findings paved a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth, boosting the development of green farming.					
36374637	3	54	theme	isolate	518:524	arg1	film					567:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	0	55	theme	oxidized	71:78	arg1	sucrose					80:86	oxidized sucrose	71:86	oxidized sucrose as agricultural mulch films for green farming	71:132	All-biodegradable soy protein isolate/lignin composite cross-linked by oxidized sucrose as agricultural mulch films for green farming.					
36374637	3	56	theme	green	617:621	arg1	cross-linker					623:634	green cross-linker	617:634	green cross-linker	617:634	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	3	57	theme	sucrose	541:547	arg1	film					567:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	6	58	theme	mulch	1285:1289	arg1	film					1291:1294	such multifunctional mulch film	1264:1294	such multifunctional mulch film compared to traditional low-density PE film	1264:1338	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	7	59	dep	designing	1384:1392	arg1	film					1461:1464	eco-friendly, low-cost and all-biodegradable mulch film	1410:1464	eco-friendly, low-cost and all-biodegradable mulch film	1410:1464	Our findings paved a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth, boosting the development of green farming.					
36374637	1	60	theme	discarded	167:175	arg1	film					210:213	the discarded traditional non-degradable mulch film	163:213	the discarded traditional non-degradable mulch film	163:213	Microplastics produced from the discarded traditional non-degradable mulch film deteriorate the ecological environment and pose a great threat to human health.					
36374637	7	61	theme	mulch	1455:1459	arg1	film					1461:1464	eco-friendly, low-cost and all-biodegradable mulch film	1410:1464	eco-friendly, low-cost and all-biodegradable mulch film	1410:1464	Our findings paved a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth, boosting the development of green farming.					
36374637	4	62	theme	sacrificial	769:779	arg1	bonds					781:785	sacrificial bonds	769:785	sacrificial bonds for energy dissipation	769:808	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	62	theme	sacrificial	769:779	arg1	strength					888:895	tensile strength	880:895	the intermolecular interactions as well as tensile strength	837:895	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	62	theme	sacrificial	769:779	arg1	interactions					856:867	the intermolecular interactions	837:867	the intermolecular interactions as well as tensile strength	837:895	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	62	theme	sacrificial	769:779	arg1	effect					716:721	The cross-linking effect and hydrogen bonds	698:740	effect	716:721	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	62	theme	sacrificial	769:779	arg1	bonds					736:740	The cross-linking effect and hydrogen bonds	698:740	bonds	736:740	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	5	63	theme	urea	1104:1107	arg1	release					1109:1115	sustained urea release	1094:1115	sustained urea release	1094:1115	Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties.					
36374637	6	64	theme	multifunctional	1269:1283	arg1	film					1291:1294	such multifunctional mulch film	1264:1294	such multifunctional mulch film compared to traditional low-density PE film	1264:1338	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	5	65	theme	effect	1083:1088	arg1	properties					1117:1126	excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties	1021:1126	excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties	1021:1126	Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties.					
36374637	7	66	theme	farming	1532:1538	arg1	development					1511:1521	the development	1507:1521	the development of green farming	1507:1538	Our findings paved a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth, boosting the development of green farming.					
36374637	4	67	theme	energy	791:796	arg1	dissipation					798:808	energy dissipation	791:808	energy dissipation	791:808	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	6	68	theme	such	1264:1267	arg1	film					1291:1294	such multifunctional mulch film	1264:1294	such multifunctional mulch film compared to traditional low-density PE film	1264:1338	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	6	69	theme	low-density	1320:1330	arg1	film					1335:1338	traditional low-density PE film	1308:1338	traditional low-density PE film	1308:1338	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	3	70	theme	chemical/physical	667:683	arg1	interaction					685:695	chemical/physical interaction	667:695	chemical/physical interaction	667:695	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	2	71	theme	mulch	381:385	arg1	film					387:390	traditional plastic mulch film	361:390	traditional plastic mulch film highly contributed to the progress of green agriculture	361:446	Developing eco-friendly and biodegradable materials to substitute traditional plastic mulch film highly contributed to the progress of green agriculture.					
36374637	6	72	theme	natural	1236:1242	arg1	soil					1244:1247	the natural soil	1232:1247	the natural soil	1232:1247	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	1	73	theme	great	265:269	arg1	threat					271:276	a great threat	263:276	a great threat to human health	263:292	Microplastics produced from the discarded traditional non-degradable mulch film deteriorate the ecological environment and pose a great threat to human health.					
36374637	5	74	theme	heat	1065:1068	arg1	effect					1083:1088	heat preservation effect	1065:1088	heat preservation effect	1065:1088	Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties.					
36374637	5	75	theme	sustained	1094:1102	arg1	release					1109:1115	sustained urea release	1094:1115	sustained urea release	1094:1115	Moreover, the SPI/OS/Lignin mulch film also presented excellent UV-shielding, moisture retention, heat preservation effect and sustained urea release properties.					
36374637	7	76	theme	eco-friendly	1410:1421	arg1	film					1461:1464	eco-friendly, low-cost and all-biodegradable mulch film	1410:1464	eco-friendly, low-cost and all-biodegradable mulch film	1410:1464	Our findings paved a solid way in rational designing and fabricating eco-friendly, low-cost and all-biodegradable mulch film to facilitate the crops growth, boosting the development of green farming.					
36374637	3	77	theme	/Lignin	553:559	arg1	film					567:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film	471:570	Herein, a category of eco-friendly and all-biodegradable soy protein isolate (SPI)/oxidized sucrose (OS)/Lignin mulch film was innovatively proposed by employing OS as green cross-linker and lignin as nanofiller under chemical/physical interaction.					
36374637	6	78	theme	germination	1181:1191	arg1	rate					1193:1196	higher germination rate	1174:1196	higher germination rate of cabbage seed	1174:1212	Benefitting from the above-mentioned merits, higher germination rate of cabbage seed was achieved when the natural soil was covered by such multifunctional mulch film compared to traditional low-density PE film.					
36374637	4	79	theme	tensile	880:886	arg1	bonds					781:785	sacrificial bonds	769:785	sacrificial bonds for energy dissipation	769:808	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	79	theme	tensile	880:886	arg1	strength					888:895	tensile strength	880:895	the intermolecular interactions as well as tensile strength	837:895	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	79	theme	tensile	880:886	arg1	interactions					856:867	the intermolecular interactions	837:867	the intermolecular interactions as well as tensile strength	837:895	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	79	theme	tensile	880:886	arg1	effect					716:721	The cross-linking effect and hydrogen bonds	698:740	effect	716:721	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	4	79	theme	tensile	880:886	arg1	bonds					736:740	The cross-linking effect and hydrogen bonds	698:740	bonds	736:740	The cross-linking effect and hydrogen bonds between biopolymers act as sacrificial bonds for energy dissipation and effectively reinforced the intermolecular interactions as well as tensile strength from 6.67 MPa of pristine SPI film to 8.45 MPa of SPI/OS/Lignin film.					
36374637	2	80	dep	eco-friendly	306:317	arg1	materials					337:345	materials	337:345	materials	337:345	Developing eco-friendly and biodegradable materials to substitute traditional plastic mulch film highly contributed to the progress of green agriculture.					
36087275	3	0	theme	revision	548:555	arg1	surgery					557:563	revision surgery	548:563	revision surgery	548:563	The difficulty and high cost of curing periprosthetic joint infection (PJI) and revision surgery limit the further clinical application of tantalum.					
36087275	1	1	theme	osteoinductive	292:305	arg1	ability					307:313	good osteoinductive ability	287:313	good osteoinductive ability	287:313	3D-printed porous tantalum scaffold has been increasingly used in arthroplasty due to its bone-matching elastic modulus and good osteoinductive ability.					
36087275	4	2	theme	vancomycin-loaded	642:658	arg1	scaffolds					676:684	vancomycin-loaded porous tantalum scaffolds	642:684	vancomycin-loaded porous tantalum scaffolds	642:684	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	7	3	from	control	1383:1389	arg1	tissue					1399:1404	soft tissue	1394:1404	soft tissue	1394:1404	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	6	4	from	generation	1131:1140	arg1	surface					1238:1244	the surface	1234:1244	the surface of scaffolds	1234:1257	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	3	5	theme	further	575:581	arg1	application					592:602	the further clinical application	571:602	the further clinical application of tantalum	571:614	The difficulty and high cost of curing periprosthetic joint infection (PJI) and revision surgery limit the further clinical application of tantalum.					
36087275	2	6	theme	ability	351:357	arg1	lack					329:332	the lack	325:332	the lack of antibacterial ability	325:357	However, the lack of antibacterial ability makes it difficult for tantalum to prevent the occurrence and development of periprosthetic joint infection.					
36087275	5	7	theme	adherent	939:946	arg1	bacteria					948:955	initially adherent bacteria	929:955	initially adherent bacteria	929:955	Our in vitro experiments show that the scaffold achieves rapid killing of initially adherent bacteria and effectively prevents biofilm formation.					
36087275	6	8	theme	original	1045:1052	arg1	structure					1064:1072	original excellent structure	1045:1072	original excellent structure	1045:1072	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	6	9	theme	scaffolds	1249:1257	arg1	surface					1238:1244	the surface	1234:1244	the surface of scaffolds	1234:1257	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	0	10	theme	Antibiofilm	139:149	arg1	Properties					151:160	Antibiofilm Properties	139:160	Antibiofilm Properties	139:160	Biofunctionalization of 3D Printed Porous Tantalum Using a Vancomycin-Carboxymethyl Chitosan Composite Coating to Improve Osteogenesis and Antibiofilm Properties.					
36087275	3	11	theme	tantalum	607:614	arg1	application					592:602	the further clinical application	571:602	the further clinical application of tantalum	571:614	The difficulty and high cost of curing periprosthetic joint infection (PJI) and revision surgery limit the further clinical application of tantalum.					
36087275	5	12	theme	rapid	912:916	arg1	killing					918:924	rapid killing	912:924	rapid killing of initially adherent bacteria	912:955	Our in vitro experiments show that the scaffold achieves rapid killing of initially adherent bacteria and effectively prevents biofilm formation.					
36087275	6	13	theme	stem	1220:1223	arg1	cells					1225:1229	mesenchymal stem cells	1208:1229	mesenchymal stem cells	1208:1229	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	8	14	theme	clinical	1592:1599	arg1	potential					1613:1621	great clinical application potential	1586:1621	great clinical application potential	1586:1621	Combined with the economic friendliness and practicality of its preparation, this scaffold has great clinical application potential in the treatment of periprosthetic joint infection.					
36087275	7	15	theme	early	1478:1482	arg1	stage					1484:1488	an early stage	1475:1488	an early stage	1475:1488	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	1	16	theme	3D-printed	163:172	arg1	scaffold					190:197	3D-printed porous tantalum scaffold	163:197	3D-printed porous tantalum scaffold	163:197	3D-printed porous tantalum scaffold has been increasingly used in arthroplasty due to its bone-matching elastic modulus and good osteoinductive ability.					
36087275	6	17	theme	mineralized	1145:1155	arg1	matrix					1157:1162	mineralized matrix	1145:1162	mineralized matrix	1145:1162	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	7	18	theme	bacterial	1346:1354	arg1	clearance					1356:1364	efficient bacterial clearance	1336:1364	efficient bacterial clearance	1336:1364	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	7	19	theme	rat	1270:1272	arg1	model					1297:1301	a rat subcutaneous infection model	1268:1301	a rat subcutaneous infection model	1268:1301	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	1	20	theme	tantalum	181:188	arg1	scaffold					190:197	3D-printed porous tantalum scaffold	163:197	3D-printed porous tantalum scaffold	163:197	3D-printed porous tantalum scaffold has been increasingly used in arthroplasty due to its bone-matching elastic modulus and good osteoinductive ability.					
36087275	6	21	theme	porous	1098:1103	arg1	tantalum					1105:1112	porous tantalum	1098:1112	porous tantalum	1098:1112	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	6	22	dep	structure	1064:1072	arg1	the					1041:1043	the	1041:1043	the	1041:1043	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	0	23	theme	Composite	93:101	arg1	Coating					103:109	a Vancomycin-Carboxymethyl Chitosan Composite Coating	57:109	a Vancomycin-Carboxymethyl Chitosan Composite Coating to Improve Osteogenesis and Antibiofilm Properties	57:160	Biofunctionalization of 3D Printed Porous Tantalum Using a Vancomycin-Carboxymethyl Chitosan Composite Coating to Improve Osteogenesis and Antibiofilm Properties.					
36087275	2	24	dep	prevent	394:400	arg1	tantalum					382:389	tantalum	382:389	tantalum	382:389	However, the lack of antibacterial ability makes it difficult for tantalum to prevent the occurrence and development of periprosthetic joint infection.					
36087275	2	24	dep	prevent	394:400	arg1	difficult					368:376	difficult	368:376	difficult	368:376	However, the lack of antibacterial ability makes it difficult for tantalum to prevent the occurrence and development of periprosthetic joint infection.					
36087275	7	25	theme	composite	1308:1316	arg1	bioscaffold					1318:1328	the composite bioscaffold	1304:1328	the composite bioscaffold	1304:1328	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	8	26	theme	joint	1658:1662	arg1	infection					1664:1672	periprosthetic joint infection	1643:1672	periprosthetic joint infection	1643:1672	Combined with the economic friendliness and practicality of its preparation, this scaffold has great clinical application potential in the treatment of periprosthetic joint infection.					
36087275	2	27	dep	occurrence	406:415	arg1	the					402:404	the	402:404	the	402:404	However, the lack of antibacterial ability makes it difficult for tantalum to prevent the occurrence and development of periprosthetic joint infection.					
36087275	2	28	theme	periprosthetic	436:449	arg1	infection					457:465	periprosthetic joint infection	436:465	periprosthetic joint infection	436:465	However, the lack of antibacterial ability makes it difficult for tantalum to prevent the occurrence and development of periprosthetic joint infection.					
36087275	6	29	theme	osteogenesis-related	1168:1187	arg1	expression					1194:1203	osteogenesis-related gene expression	1168:1203	osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds	1168:1257	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	0	30	theme	Chitosan	84:91	arg1	Coating					103:109	a Vancomycin-Carboxymethyl Chitosan Composite Coating	57:109	a Vancomycin-Carboxymethyl Chitosan Composite Coating to Improve Osteogenesis and Antibiofilm Properties	57:160	Biofunctionalization of 3D Printed Porous Tantalum Using a Vancomycin-Carboxymethyl Chitosan Composite Coating to Improve Osteogenesis and Antibiofilm Properties.					
36087275	4	31	theme	carboxymethyl	797:809	arg1	chitosan					811:818	carboxymethyl chitosan	797:818	carboxymethyl chitosan	797:818	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	7	32	theme	infection	1287:1295	arg1	model					1297:1301	a rat subcutaneous infection model	1268:1301	a rat subcutaneous infection model	1268:1301	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	6	33	theme	matrix	1157:1162	arg1	expression					1194:1203	osteogenesis-related gene expression	1168:1203	osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds	1168:1257	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	6	33	theme	matrix	1157:1162	arg1	generation					1131:1140	the generation	1127:1140	the generation of mineralized matrix	1127:1162	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	3	34	theme	periprosthetic	507:520	arg1	PJI					539:541	PJI	539:541	PJI	539:541	The difficulty and high cost of curing periprosthetic joint infection (PJI) and revision surgery limit the further clinical application of tantalum.					
36087275	3	34	theme	periprosthetic	507:520	arg1	infection					528:536	periprosthetic joint infection	507:536	periprosthetic joint infection (PJI)	507:542	The difficulty and high cost of curing periprosthetic joint infection (PJI) and revision surgery limit the further clinical application of tantalum.					
36087275	0	35	theme	3D	24:25	arg1	Tantalum					42:49	3D Printed Porous Tantalum	24:49	3D Printed Porous Tantalum	24:49	Biofunctionalization of 3D Printed Porous Tantalum Using a Vancomycin-Carboxymethyl Chitosan Composite Coating to Improve Osteogenesis and Antibiofilm Properties.					
36087275	1	36	theme	porous	174:179	arg1	scaffold					190:197	3D-printed porous tantalum scaffold	163:197	3D-printed porous tantalum scaffold	163:197	3D-printed porous tantalum scaffold has been increasingly used in arthroplasty due to its bone-matching elastic modulus and good osteoinductive ability.					
36087275	0	37	theme	Vancomycin-Carboxymethyl	59:82	arg1	Coating					103:109	a Vancomycin-Carboxymethyl Chitosan Composite Coating	57:109	a Vancomycin-Carboxymethyl Chitosan Composite Coating to Improve Osteogenesis and Antibiofilm Properties	57:160	Biofunctionalization of 3D Printed Porous Tantalum Using a Vancomycin-Carboxymethyl Chitosan Composite Coating to Improve Osteogenesis and Antibiofilm Properties.					
36087275	2	38	theme	infection	457:465	arg1	development					421:431	development	421:431	development	421:431	However, the lack of antibacterial ability makes it difficult for tantalum to prevent the occurrence and development of periprosthetic joint infection.					
36087275	2	38	theme	infection	457:465	arg1	occurrence					406:415	occurrence	406:415	occurrence	406:415	However, the lack of antibacterial ability makes it difficult for tantalum to prevent the occurrence and development of periprosthetic joint infection.					
36087275	0	39	theme	Porous	35:40	arg1	Tantalum					42:49	3D Printed Porous Tantalum	24:49	3D Printed Porous Tantalum	24:49	Biofunctionalization of 3D Printed Porous Tantalum Using a Vancomycin-Carboxymethyl Chitosan Composite Coating to Improve Osteogenesis and Antibiofilm Properties.					
36087275	3	40	theme	high	487:490	arg1	cost					492:495	high cost	487:495	high cost	487:495	The difficulty and high cost of curing periprosthetic joint infection (PJI) and revision surgery limit the further clinical application of tantalum.					
36087275	5	41	theme	in	859:860	arg1	experiments					868:878	Our in vitro experiments	855:878	Our in vitro experiments	855:878	Our in vitro experiments show that the scaffold achieves rapid killing of initially adherent bacteria and effectively prevents biofilm formation.					
36087275	1	42	theme	bone-matching	253:265	arg1	modulus					275:281	its bone-matching elastic modulus	249:281	its bone-matching elastic modulus	249:281	3D-printed porous tantalum scaffold has been increasingly used in arthroplasty due to its bone-matching elastic modulus and good osteoinductive ability.					
36087275	7	43	theme	soft	1394:1397	arg1	tissue					1399:1404	soft tissue	1394:1404	soft tissue	1394:1404	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	4	44	theme	porous	660:665	arg1	scaffolds					676:684	vancomycin-loaded porous tantalum scaffolds	642:684	vancomycin-loaded porous tantalum scaffolds	642:684	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	6	45	from	expression	1194:1203	arg1	surface					1238:1244	the surface	1234:1244	the surface of scaffolds	1234:1257	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	6	46	theme	mesenchymal	1208:1218	arg1	cells					1225:1229	mesenchymal stem cells	1208:1229	mesenchymal stem cells	1208:1229	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	8	47	contain	has	1582:1584	arg2	potential					1613:1621	great clinical application potential	1586:1621	great clinical application potential	1586:1621	Combined with the economic friendliness and practicality of its preparation, this scaffold has great clinical application potential in the treatment of periprosthetic joint infection.					
36087275	8	47	contain	has	1582:1584	arg1	scaffold					1573:1580	this scaffold	1568:1580	this scaffold	1568:1580	Combined with the economic friendliness and practicality of its preparation, this scaffold has great clinical application potential in the treatment of periprosthetic joint infection.					
36087275	1	48	theme	good	287:290	arg1	ability					307:313	good osteoinductive ability	287:313	good osteoinductive ability	287:313	3D-printed porous tantalum scaffold has been increasingly used in arthroplasty due to its bone-matching elastic modulus and good osteoinductive ability.					
36087275	7	49	from	clearance	1356:1364	arg1	tissue					1399:1404	soft tissue	1394:1404	soft tissue	1394:1404	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	3	50	theme	clinical	583:590	arg1	application					592:602	the further clinical application	571:602	the further clinical application of tantalum	571:614	The difficulty and high cost of curing periprosthetic joint infection (PJI) and revision surgery limit the further clinical application of tantalum.					
36087275	5	51	dep	in	859:860	arg1	vitro					862:866	vitro	862:866	vitro	862:866	Our in vitro experiments show that the scaffold achieves rapid killing of initially adherent bacteria and effectively prevents biofilm formation.					
36087275	6	52	theme	excellent	1054:1062	arg1	structure					1064:1072	original excellent structure	1045:1072	original excellent structure	1045:1072	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	4	53	theme	3-aminopropyl	725:737	arg1	APTES					756:760	APTES	756:760	APTES	756:760	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	4	53	theme	3-aminopropyl	725:737	arg1	triethoxysilane					739:753	(3-aminopropyl)triethoxysilane	724:753	(3-aminopropyl)triethoxysilane (APTES)	724:761	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	2	54	theme	antibacterial	337:349	arg1	ability					351:357	antibacterial ability	337:357	antibacterial ability	337:357	However, the lack of antibacterial ability makes it difficult for tantalum to prevent the occurrence and development of periprosthetic joint infection.					
36087275	8	55	theme	great	1586:1590	arg1	potential					1613:1621	great clinical application potential	1586:1621	great clinical application potential	1586:1621	Combined with the economic friendliness and practicality of its preparation, this scaffold has great clinical application potential in the treatment of periprosthetic joint infection.					
36087275	4	56	theme	chemical	703:710	arg1	grafting					712:719	the chemical grafting	699:719	the chemical grafting of (3-aminopropyl)triethoxysilane (APTES)	699:761	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	5	57	theme	biofilm	982:988	arg1	formation					990:998	biofilm formation	982:998	biofilm formation	982:998	Our in vitro experiments show that the scaffold achieves rapid killing of initially adherent bacteria and effectively prevents biofilm formation.					
36087275	8	58	theme	application	1601:1611	arg1	potential					1613:1621	great clinical application potential	1586:1621	great clinical application potential	1586:1621	Combined with the economic friendliness and practicality of its preparation, this scaffold has great clinical application potential in the treatment of periprosthetic joint infection.					
36087275	1	59	used	used	221:224	arg2	scaffold					190:197	3D-printed porous tantalum scaffold	163:197	3D-printed porous tantalum scaffold	163:197	3D-printed porous tantalum scaffold has been increasingly used in arthroplasty due to its bone-matching elastic modulus and good osteoinductive ability.					
36087275	4	60	theme	electrostatic	771:783	arg1	assembly					785:792	the electrostatic assembly	767:792	the electrostatic assembly of carboxymethyl chitosan and vancomycin	767:833	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	7	61	theme	efficient	1336:1344	arg1	clearance					1356:1364	efficient bacterial clearance	1336:1364	efficient bacterial clearance	1336:1364	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	5	62	theme	bacteria	948:955	arg1	killing					918:924	rapid killing	912:924	rapid killing of initially adherent bacteria	912:955	Our in vitro experiments show that the scaffold achieves rapid killing of initially adherent bacteria and effectively prevents biofilm formation.					
36087275	6	63	theme	tantalum	1105:1112	arg1	biocompatibility					1078:1093	biocompatibility	1078:1093	biocompatibility	1078:1093	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	6	63	theme	tantalum	1105:1112	arg1	structure					1064:1072	original excellent structure	1045:1072	original excellent structure	1045:1072	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	4	64	theme	triethoxysilane	739:753	arg1	grafting					712:719	the chemical grafting	699:719	the chemical grafting of (3-aminopropyl)triethoxysilane (APTES)	699:761	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	4	64	theme	triethoxysilane	739:753	arg1	assembly					785:792	the electrostatic assembly	767:792	the electrostatic assembly of carboxymethyl chitosan and vancomycin	767:833	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	8	65	dep	friendliness	1518:1529	arg1	the					1505:1507	the	1505:1507	the	1505:1507	Combined with the economic friendliness and practicality of its preparation, this scaffold has great clinical application potential in the treatment of periprosthetic joint infection.					
36087275	8	66	theme	periprosthetic	1643:1656	arg1	infection					1664:1672	periprosthetic joint infection	1643:1672	periprosthetic joint infection	1643:1672	Combined with the economic friendliness and practicality of its preparation, this scaffold has great clinical application potential in the treatment of periprosthetic joint infection.					
36087275	8	67	theme	infection	1664:1672	arg1	treatment					1630:1638	the treatment	1626:1638	the treatment of periprosthetic joint infection	1626:1672	Combined with the economic friendliness and practicality of its preparation, this scaffold has great clinical application potential in the treatment of periprosthetic joint infection.					
36087275	4	68	theme	vancomycin	824:833	arg1	grafting					712:719	the chemical grafting	699:719	the chemical grafting of (3-aminopropyl)triethoxysilane (APTES)	699:761	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	4	68	theme	vancomycin	824:833	arg1	assembly					785:792	the electrostatic assembly	767:792	the electrostatic assembly of carboxymethyl chitosan and vancomycin	767:833	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	7	69	theme	tissue	1458:1463	arg1	repair					1465:1470	tissue repair	1458:1470	tissue repair at an early stage	1458:1488	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	7	70	theme	subcutaneous	1274:1285	arg1	model					1297:1301	a rat subcutaneous infection model	1268:1301	a rat subcutaneous infection model	1268:1301	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	7	71	from	stage	1484:1488	arg1	repair					1465:1470	tissue repair	1458:1470	tissue repair at an early stage	1458:1488	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	7	72	theme	suitable	1445:1452	arg1	microenvironment					1428:1443	an immune microenvironment	1418:1443	an immune microenvironment suitable for tissue repair at an early stage	1418:1488	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	0	73	theme	Printed	27:33	arg1	Tantalum					42:49	3D Printed Porous Tantalum	24:49	3D Printed Porous Tantalum	24:49	Biofunctionalization of 3D Printed Porous Tantalum Using a Vancomycin-Carboxymethyl Chitosan Composite Coating to Improve Osteogenesis and Antibiofilm Properties.					
36087275	7	74	theme	immune	1421:1426	arg1	microenvironment					1428:1443	an immune microenvironment	1418:1443	an immune microenvironment suitable for tissue repair at an early stage	1418:1488	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	4	75	theme	first	843:847	arg1	time					849:852	the first time	839:852	the first time	839:852	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	4	76	theme	chitosan	811:818	arg1	grafting					712:719	the chemical grafting	699:719	the chemical grafting of (3-aminopropyl)triethoxysilane (APTES)	699:761	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	4	76	theme	chitosan	811:818	arg1	assembly					785:792	the electrostatic assembly	767:792	the electrostatic assembly of carboxymethyl chitosan and vancomycin	767:833	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	3	77	theme	joint	522:526	arg1	PJI					539:541	PJI	539:541	PJI	539:541	The difficulty and high cost of curing periprosthetic joint infection (PJI) and revision surgery limit the further clinical application of tantalum.					
36087275	3	77	theme	joint	522:526	arg1	infection					528:536	periprosthetic joint infection	507:536	periprosthetic joint infection (PJI)	507:542	The difficulty and high cost of curing periprosthetic joint infection (PJI) and revision surgery limit the further clinical application of tantalum.					
36087275	0	78	theme	Tantalum	42:49	arg1	Biofunctionalization					0:19	Biofunctionalization	0:19	Biofunctionalization of 3D Printed Porous Tantalum	0:49	Biofunctionalization of 3D Printed Porous Tantalum Using a Vancomycin-Carboxymethyl Chitosan Composite Coating to Improve Osteogenesis and Antibiofilm Properties.					
36087275	3	79	dep	difficulty	472:481	arg1	The					468:470	The	468:470	The	468:470	The difficulty and high cost of curing periprosthetic joint infection (PJI) and revision surgery limit the further clinical application of tantalum.					
36087275	6	80	theme	gene	1189:1192	arg1	expression					1194:1203	osteogenesis-related gene expression	1168:1203	osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds	1168:1257	In addition, our modification preserves the original excellent structure and biocompatibility of porous tantalum and promotes the generation of mineralized matrix and osteogenesis-related gene expression by mesenchymal stem cells on the surface of scaffolds.					
36087275	8	81	theme	economic	1509:1516	arg1	friendliness					1518:1529	economic friendliness	1509:1529	economic friendliness	1509:1529	Combined with the economic friendliness and practicality of its preparation, this scaffold has great clinical application potential in the treatment of periprosthetic joint infection.					
36087275	2	82	theme	joint	451:455	arg1	infection					457:465	periprosthetic joint infection	436:465	periprosthetic joint infection	436:465	However, the lack of antibacterial ability makes it difficult for tantalum to prevent the occurrence and development of periprosthetic joint infection.					
36087275	4	83	theme	tantalum	667:674	arg1	scaffolds					676:684	vancomycin-loaded porous tantalum scaffolds	642:684	vancomycin-loaded porous tantalum scaffolds	642:684	Therefore, we fabricated vancomycin-loaded porous tantalum scaffolds by combining the chemical grafting of (3-aminopropyl)triethoxysilane (APTES) and the electrostatic assembly of carboxymethyl chitosan and vancomycin for the first time.					
36087275	1	84	theme	elastic	267:273	arg1	modulus					275:281	its bone-matching elastic modulus	249:281	its bone-matching elastic modulus	249:281	3D-printed porous tantalum scaffold has been increasingly used in arthroplasty due to its bone-matching elastic modulus and good osteoinductive ability.					
36087275	7	85	theme	inflammation	1370:1381	arg1	control					1383:1389	inflammation control	1370:1389	inflammation control	1370:1389	Through a rat subcutaneous infection model, the composite bioscaffold shows efficient bacterial clearance and inflammation control in soft tissue and creates an immune microenvironment suitable for tissue repair at an early stage.					
36087275	8	86	theme	preparation	1555:1565	arg1	practicality					1535:1546	practicality	1535:1546	practicality	1535:1546	Combined with the economic friendliness and practicality of its preparation, this scaffold has great clinical application potential in the treatment of periprosthetic joint infection.					
36087275	8	86	theme	preparation	1555:1565	arg1	friendliness					1518:1529	economic friendliness	1509:1529	economic friendliness	1509:1529	Combined with the economic friendliness and practicality of its preparation, this scaffold has great clinical application potential in the treatment of periprosthetic joint infection.					
36596207	2	0	theme	hepatic	384:390	arg1	steatosis					392:400	high-sucrose diet-induced hepatic steatosis	358:400	high-sucrose diet-induced hepatic steatosis	358:400	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	6	1	theme	tryptophan	1379:1388	arg1	metabolism					1390:1399	tryptophan metabolism	1379:1399	tryptophan metabolism	1379:1399	Functional prediction showed that tryptophan metabolism was one of the key metabolic pathways affected by gut microbiota changes.					
36596207	8	2	theme	barrier	1911:1917	arg1	integrity					1919:1927	the intestinal barrier integrity	1896:1927	the intestinal barrier integrity	1896:1927	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	3	3	theme	intestinal	654:663	arg1	integrity					673:681	the intestinal barrier integrity	650:681	the intestinal barrier integrity	650:681	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	1	4	theme	action	273:278	arg1	mechanisms					280:289	its action mechanisms	269:289	its action mechanisms	269:289	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	6	5	theme	gut	1451:1453	arg1	changes					1466:1472	gut microbiota changes	1451:1472	gut microbiota changes	1451:1472	Functional prediction showed that tryptophan metabolism was one of the key metabolic pathways affected by gut microbiota changes.					
36596207	1	6	theme	prebiotic	193:201	arg1	inulin					203:208	prebiotic inulin	193:208	prebiotic inulin	193:208	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	7	7	theme	5-hydoxyindoleacetic	1693:1712	arg1	acid					1714:1717	5-hydoxyindoleacetic acid	1693:1717	5-hydoxyindoleacetic acid	1693:1717	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	6	8	theme	Functional	1345:1354	arg1	prediction					1356:1365	Functional prediction	1345:1365	Functional prediction	1345:1365	Functional prediction showed that tryptophan metabolism was one of the key metabolic pathways affected by gut microbiota changes.					
36596207	2	9	theme	inulin	315:320	arg1	consumption					322:332	inulin consumption	315:332	inulin consumption	315:332	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	7	10	theme	kynurenic	1629:1637	arg1	acid					1639:1642	kynurenic acid	1629:1642	kynurenic acid	1629:1642	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	5	11	theme	faecal	1250:1255	arg1	levels					1265:1270	faecal acetate levels	1250:1270	faecal acetate levels	1250:1270	SCFA quantitative analysis showed that dietary inulin suppressed faecal acetate levels, but improved propionate and butyrate concentrations in rats with NAFLD.					
36596207	1	12	theme	fatty	226:230	arg1	NAFLD					247:251	NAFLD	247:251	NAFLD	247:251	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	1	12	theme	fatty	226:230	arg1	disease					238:244	nonalcoholic fatty liver disease	213:244	nonalcoholic fatty liver disease (NAFLD)	213:252	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	6	13	theme	pathways	1430:1437	arg1	one					1405:1407	one	1405:1407	one	1405:1407	Functional prediction showed that tryptophan metabolism was one of the key metabolic pathways affected by gut microbiota changes.					
36596207	6	13	theme	pathways	1430:1437	arg1	pathways					1430:1437	the key metabolic pathways	1412:1437	the key metabolic pathways affected by gut microbiota changes	1412:1472	Functional prediction showed that tryptophan metabolism was one of the key metabolic pathways affected by gut microbiota changes.					
36596207	6	14	theme	key	1416:1418	arg1	pathways					1430:1437	the key metabolic pathways	1412:1437	the key metabolic pathways affected by gut microbiota changes	1412:1472	Functional prediction showed that tryptophan metabolism was one of the key metabolic pathways affected by gut microbiota changes.					
36596207	1	15	theme	Increasing	132:141	arg1	evidence					143:150	Increasing evidence	132:150	Increasing evidence	132:150	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	4	16	theme	inulin-treated	1065:1078	arg1	rats					1080:1083	the inulin-treated rats	1061:1083	the inulin-treated rats	1061:1083	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	7	17	theme	faecal	1585:1590	arg1	contents					1592:1599	faecal contents	1585:1599	faecal contents of indole-3-acetic acid and kynurenic acid	1585:1642	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	5	18	theme	butyrate	1301:1308	arg1	concentrations					1310:1323	propionate and butyrate concentrations	1286:1323	propionate and butyrate concentrations in rats with NAFLD	1286:1342	SCFA quantitative analysis showed that dietary inulin suppressed faecal acetate levels, but improved propionate and butyrate concentrations in rats with NAFLD.					
36596207	3	19	theme	junction	734:741	arg1	occludin					787:794	occludin	787:794	occludin	787:794	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	3	19	theme	junction	734:741	arg1	claudin-1					773:781	claudin-1	773:781	claudin-1	773:781	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	3	19	theme	junction	734:741	arg1	proteins					743:750	tight junction proteins	728:750	tight junction proteins (zonula occludens-1, claudin-1 and occludin)	728:795	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	3	19	theme	junction	734:741	arg1	occludens-1					760:770	zonula occludens-1	753:770	zonula occludens-1	753:770	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	2	20	theme	proliferator-activated	566:587	arg1	receptor					589:596	peroxisome proliferator-activated receptor	555:596	peroxisome proliferator-activated receptor alpha	555:602	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	5	21	with	rats	1328:1331	arg1	NAFLD					1338:1342	NAFLD	1338:1342	NAFLD	1338:1342	SCFA quantitative analysis showed that dietary inulin suppressed faecal acetate levels, but improved propionate and butyrate concentrations in rats with NAFLD.					
36596207	4	22	theme	short-chain	928:938	arg1	SCFA					952:955	SCFA	952:955	SCFA	952:955	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	4	22	theme	short-chain	928:938	arg1	acid					946:949	short-chain fatty acid	928:949	short-chain fatty acid (SCFA)-producers	928:966	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	2	23	theme	liver	438:442	arg1	protein					516:522	carbohydrate response element-binding protein	478:522	carbohydrate response element-binding protein	478:522	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	2	23	theme	liver	438:442	arg1	regulators					456:465	rehabilitated liver lipogenesis regulators	424:465	rehabilitated liver lipogenesis regulators	424:465	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	2	23	theme	liver	438:442	arg1	alpha					598:602	peroxisome proliferator-activated receptor alpha	555:602	peroxisome proliferator-activated receptor alpha	555:602	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	2	23	theme	liver	438:442	arg1	desaturase-1					538:549	stearoyl-CoA desaturase-1	525:549	stearoyl-CoA desaturase-1	525:549	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	0	24	theme	barrier	114:120	arg1	function					122:129	intestinal barrier function	103:129	intestinal barrier function	103:129	Inulin intervention attenuates hepatic steatosis in rats via modulating gut microbiota and maintaining intestinal barrier function.					
36596207	5	25	theme	propionate	1286:1295	arg1	concentrations					1310:1323	propionate and butyrate concentrations	1286:1323	propionate and butyrate concentrations in rats with NAFLD	1286:1342	SCFA quantitative analysis showed that dietary inulin suppressed faecal acetate levels, but improved propionate and butyrate concentrations in rats with NAFLD.					
36596207	7	26	theme	inulin	1552:1557	arg1	intervention					1559:1570	inulin intervention	1552:1570	inulin intervention	1552:1570	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	0	27	from	steatosis	39:47	arg1	rats					52:55	rats	52:55	rats	52:55	Inulin intervention attenuates hepatic steatosis in rats via modulating gut microbiota and maintaining intestinal barrier function.					
36596207	8	28	dep	prevention	1971:1980	arg1	the					1967:1969	the	1967:1969	the	1967:1969	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	4	29	theme	microbiota	883:892	arg1	composition					894:904	the gut microbiota composition	875:904	the gut microbiota composition	875:904	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	0	30	theme	Inulin	0:5	arg1	intervention					7:18	Inulin intervention	0:18	Inulin intervention	0:18	Inulin intervention attenuates hepatic steatosis in rats via modulating gut microbiota and maintaining intestinal barrier function.					
36596207	2	31	theme	response	491:498	arg1	protein					516:522	carbohydrate response element-binding protein	478:522	carbohydrate response element-binding protein	478:522	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	8	32	theme	hepatic	1799:1805	arg1	steatosis					1807:1815	hepatic steatosis	1799:1815	hepatic steatosis	1799:1815	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	7	33	theme	tryptophan	1512:1521	arg1	metabolism					1523:1532	tryptophan metabolism	1512:1532	tryptophan metabolism	1512:1532	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	4	34	theme	-producers	957:966	arg1	abundance					915:923	wherein abundance	907:923	wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia,	907:1029	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	5	35	theme	quantitative	1190:1201	arg1	analysis					1203:1210	SCFA quantitative analysis	1185:1210	SCFA quantitative analysis	1185:1210	SCFA quantitative analysis showed that dietary inulin suppressed faecal acetate levels, but improved propionate and butyrate concentrations in rats with NAFLD.					
36596207	2	36	theme	stearoyl-CoA	525:536	arg1	desaturase-1					538:549	stearoyl-CoA desaturase-1	525:549	stearoyl-CoA desaturase-1	525:549	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	2	37	theme	diet-induced	371:382	arg1	steatosis					392:400	high-sucrose diet-induced hepatic steatosis	358:400	high-sucrose diet-induced hepatic steatosis	358:400	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	5	38	from	concentrations	1310:1323	arg1	rats					1328:1331	rats	1328:1331	rats with NAFLD	1328:1342	SCFA quantitative analysis showed that dietary inulin suppressed faecal acetate levels, but improved propionate and butyrate concentrations in rats with NAFLD.					
36596207	7	39	theme	targeted	1477:1484	arg1	profiling					1499:1507	A targeted metabolomics profiling	1475:1507	A targeted metabolomics profiling of tryptophan metabolism	1475:1532	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	8	40	theme	gut	1842:1844	arg1	composition					1857:1867	the gut microbiota composition	1838:1867	the gut microbiota composition	1838:1867	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	4	41	theme	acid	946:949	arg1	Phascolarctobacterium					996:1016	Phascolarctobacterium	996:1016	Phascolarctobacterium	996:1016	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	4	41	theme	acid	946:949	arg1	-producers					957:966	short-chain fatty acid (SCFA)-producers	928:966	short-chain fatty acid (SCFA)-producers	928:966	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	4	41	theme	acid	946:949	arg1	Bifidobacterium					979:993	Bifidobacterium	979:993	Bifidobacterium	979:993	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	4	41	theme	acid	946:949	arg1	Blautia					1022:1028	Blautia	1022:1028	Blautia	1022:1028	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	8	42	dep	alleviated	1788:1797	arg1	provide					1940:1946	provide	1940:1946	may provide a novel notion for the prevention and treatment of NAFLD in future	1936:2013	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	7	43	theme	NAFLD	1722:1726	arg1	rats					1728:1731	NAFLD rats	1722:1731	NAFLD rats	1722:1731	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	8	44	theme	novel	1950:1954	arg1	notion					1956:1961	a novel notion	1948:1961	a novel notion for the prevention and treatment of NAFLD in future	1948:2013	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	7	45	theme	acid	1714:1717	arg1	levels					1668:1673	levels	1668:1673	levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats	1668:1731	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	7	46	theme	kynurenine	1678:1687	arg1	levels					1668:1673	levels	1668:1673	levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats	1668:1731	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	8	47	theme	intestinal	1900:1909	arg1	integrity					1919:1927	the intestinal barrier integrity	1896:1927	the intestinal barrier integrity	1896:1927	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	6	48	theme	microbiota	1455:1464	arg1	changes					1466:1472	gut microbiota changes	1451:1472	gut microbiota changes	1451:1472	Functional prediction showed that tryptophan metabolism was one of the key metabolic pathways affected by gut microbiota changes.					
36596207	1	49	theme	inulin	203:208	arg1	efficacy					181:188	the mitigatory efficacy	166:188	the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD)	166:252	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	3	50	theme	barrier	665:671	arg1	integrity					673:681	the intestinal barrier integrity	650:681	the intestinal barrier integrity	650:681	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	1	51	theme	nonalcoholic	213:224	arg1	NAFLD					247:251	NAFLD	247:251	NAFLD	247:251	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	1	51	theme	nonalcoholic	213:224	arg1	disease					238:244	nonalcoholic fatty liver disease	213:244	nonalcoholic fatty liver disease (NAFLD)	213:252	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	7	52	theme	acid	1639:1642	arg1	contents					1592:1599	faecal contents	1585:1599	faecal contents of indole-3-acetic acid and kynurenic acid	1585:1642	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	5	53	theme	acetate	1257:1263	arg1	levels					1265:1270	faecal acetate levels	1250:1270	faecal acetate levels	1250:1270	SCFA quantitative analysis showed that dietary inulin suppressed faecal acetate levels, but improved propionate and butyrate concentrations in rats with NAFLD.					
36596207	8	54	theme	NAFLD	1999:2003	arg1	prevention					1971:1980	prevention	1971:1980	prevention	1971:1980	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	8	54	theme	NAFLD	1999:2003	arg1	treatment					1986:1994	treatment	1986:1994	treatment	1986:1994	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	3	55	dep	proteins	743:750	arg1	occludin					787:794	occludin	787:794	occludin	787:794	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	3	55	dep	proteins	743:750	arg1	claudin-1					773:781	claudin-1	773:781	claudin-1	773:781	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	3	55	dep	proteins	743:750	arg1	proteins					743:750	tight junction proteins	728:750	tight junction proteins (zonula occludens-1, claudin-1 and occludin)	728:795	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	3	55	dep	proteins	743:750	arg1	occludens-1					760:770	zonula occludens-1	753:770	zonula occludens-1	753:770	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	6	56	theme	metabolic	1420:1428	arg1	pathways					1430:1437	the key metabolic pathways	1412:1437	the key metabolic pathways affected by gut microbiota changes	1412:1472	Functional prediction showed that tryptophan metabolism was one of the key metabolic pathways affected by gut microbiota changes.					
36596207	7	57	theme	indole-3-acetic	1604:1618	arg1	acid					1620:1623	indole-3-acetic acid	1604:1623	indole-3-acetic acid	1604:1623	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	4	58	theme	inulin	843:848	arg1	administration					850:863	inulin administration	843:863	inulin administration	843:863	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	5	59	theme	dietary	1224:1230	arg1	inulin					1232:1237	dietary inulin	1224:1237	dietary inulin	1224:1237	SCFA quantitative analysis showed that dietary inulin suppressed faecal acetate levels, but improved propionate and butyrate concentrations in rats with NAFLD.					
36596207	3	60	theme	proteins	743:750	arg1	expressions					713:723	expressions	713:723	expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin)	713:795	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	0	61	theme	gut	72:74	arg1	microbiota					76:85	gut microbiota	72:85	gut microbiota	72:85	Inulin intervention attenuates hepatic steatosis in rats via modulating gut microbiota and maintaining intestinal barrier function.					
36596207	3	62	theme	tight	728:732	arg1	occludin					787:794	occludin	787:794	occludin	787:794	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	3	62	theme	tight	728:732	arg1	claudin-1					773:781	claudin-1	773:781	claudin-1	773:781	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	3	62	theme	tight	728:732	arg1	proteins					743:750	tight junction proteins	728:750	tight junction proteins (zonula occludens-1, claudin-1 and occludin)	728:795	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	3	62	theme	tight	728:732	arg1	occludens-1					760:770	zonula occludens-1	753:770	zonula occludens-1	753:770	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	2	63	theme	receptor	589:596	arg1	alpha					598:602	peroxisome proliferator-activated receptor alpha	555:602	peroxisome proliferator-activated receptor alpha	555:602	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	4	64	theme	High-throughput	798:812	arg1	sequencing					814:823	High-throughput sequencing	798:823	High-throughput sequencing	798:823	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	1	65	theme	mitigatory	170:179	arg1	efficacy					181:188	the mitigatory efficacy	166:188	the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD)	166:252	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	7	66	from	levels	1668:1673	arg1	rats					1728:1731	NAFLD rats	1722:1731	NAFLD rats	1722:1731	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	2	67	theme	peroxisome	555:564	arg1	receptor					589:596	peroxisome proliferator-activated receptor	555:596	peroxisome proliferator-activated receptor alpha	555:602	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	0	68	theme	intestinal	103:112	arg1	function					122:129	intestinal barrier function	103:129	intestinal barrier function	103:129	Inulin intervention attenuates hepatic steatosis in rats via modulating gut microbiota and maintaining intestinal barrier function.					
36596207	1	69	from	efficacy	181:188	arg1	NAFLD					247:251	NAFLD	247:251	NAFLD	247:251	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	1	69	from	efficacy	181:188	arg1	disease					238:244	nonalcoholic fatty liver disease	213:244	nonalcoholic fatty liver disease (NAFLD)	213:252	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	4	70	theme	opportunistic	1098:1110	arg1	Acinetobacter					1131:1143	Acinetobacter	1131:1143	Acinetobacter	1131:1143	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	4	70	theme	opportunistic	1098:1110	arg1	pathogens					1112:1120	opportunistic pathogens	1098:1120	opportunistic pathogens	1098:1120	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	4	70	theme	opportunistic	1098:1110	arg1	Corynebacterium_1					1149:1165	Corynebacterium_1	1149:1165	Corynebacterium_1	1149:1165	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	8	71	theme	inulin	1774:1779	arg1	intake					1781:1786	inulin intake	1774:1786	inulin intake	1774:1786	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	4	72	theme	wherein	907:913	arg1	abundance					915:923	wherein abundance	907:923	wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia,	907:1029	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	2	73	theme	rehabilitated	424:436	arg1	protein					516:522	carbohydrate response element-binding protein	478:522	carbohydrate response element-binding protein	478:522	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	2	73	theme	rehabilitated	424:436	arg1	regulators					456:465	rehabilitated liver lipogenesis regulators	424:465	rehabilitated liver lipogenesis regulators	424:465	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	2	73	theme	rehabilitated	424:436	arg1	alpha					598:602	peroxisome proliferator-activated receptor alpha	555:602	peroxisome proliferator-activated receptor alpha	555:602	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	2	73	theme	rehabilitated	424:436	arg1	desaturase-1					538:549	stearoyl-CoA desaturase-1	525:549	stearoyl-CoA desaturase-1	525:549	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	7	74	theme	metabolism	1523:1532	arg1	profiling					1499:1507	A targeted metabolomics profiling	1475:1507	A targeted metabolomics profiling of tryptophan metabolism	1475:1532	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	2	75	theme	element-binding	500:514	arg1	protein					516:522	carbohydrate response element-binding protein	478:522	carbohydrate response element-binding protein	478:522	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	4	76	theme	gut	879:881	arg1	composition					894:904	the gut microbiota composition	875:904	the gut microbiota composition	875:904	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	0	77	theme	hepatic	31:37	arg1	steatosis					39:47	hepatic steatosis	31:47	hepatic steatosis in rats	31:55	Inulin intervention attenuates hepatic steatosis in rats via modulating gut microbiota and maintaining intestinal barrier function.					
36596207	7	78	theme	acid	1620:1623	arg1	contents					1592:1599	faecal contents	1585:1599	faecal contents of indole-3-acetic acid and kynurenic acid	1585:1642	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	2	79	theme	carbohydrate	478:489	arg1	protein					516:522	carbohydrate response element-binding protein	478:522	carbohydrate response element-binding protein	478:522	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	1	80	theme	liver	232:236	arg1	NAFLD					247:251	NAFLD	247:251	NAFLD	247:251	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	1	80	theme	liver	232:236	arg1	disease					238:244	nonalcoholic fatty liver disease	213:244	nonalcoholic fatty liver disease (NAFLD)	213:252	Increasing evidence has suggested the mitigatory efficacy of prebiotic inulin on nonalcoholic fatty liver disease (NAFLD), nevertheless, its action mechanisms remain elusive.					
36596207	5	81	theme	SCFA	1185:1188	arg1	analysis					1203:1210	SCFA quantitative analysis	1185:1210	SCFA quantitative analysis	1185:1210	SCFA quantitative analysis showed that dietary inulin suppressed faecal acetate levels, but improved propionate and butyrate concentrations in rats with NAFLD.					
36596207	7	82	theme	metabolomics	1486:1497	arg1	profiling					1499:1507	A targeted metabolomics profiling	1475:1507	A targeted metabolomics profiling of tryptophan metabolism	1475:1532	A targeted metabolomics profiling of tryptophan metabolism demonstrated that inulin intervention up-regulated faecal contents of indole-3-acetic acid and kynurenic acid, whereas down-regulated levels of kynurenine and 5-hydoxyindoleacetic acid in NAFLD rats.					
36596207	3	83	theme	zonula	753:758	arg1	proteins					743:750	tight junction proteins	728:750	tight junction proteins (zonula occludens-1, claudin-1 and occludin)	728:795	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	3	83	theme	zonula	753:758	arg1	occludens-1					760:770	zonula occludens-1	753:770	zonula occludens-1	753:770	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
36596207	8	84	from	prevention	1971:1980	arg1	future					2008:2013	future	2008:2013	future	2008:2013	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	2	85	theme	lipogenesis	444:454	arg1	protein					516:522	carbohydrate response element-binding protein	478:522	carbohydrate response element-binding protein	478:522	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	2	85	theme	lipogenesis	444:454	arg1	regulators					456:465	rehabilitated liver lipogenesis regulators	424:465	rehabilitated liver lipogenesis regulators	424:465	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	2	85	theme	lipogenesis	444:454	arg1	alpha					598:602	peroxisome proliferator-activated receptor alpha	555:602	peroxisome proliferator-activated receptor alpha	555:602	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	2	85	theme	lipogenesis	444:454	arg1	desaturase-1					538:549	stearoyl-CoA desaturase-1	525:549	stearoyl-CoA desaturase-1	525:549	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	8	86	theme	microbiota	1846:1855	arg1	composition					1857:1867	the gut microbiota composition	1838:1867	the gut microbiota composition	1838:1867	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	2	87	theme	high-sucrose	358:369	arg1	steatosis					392:400	high-sucrose diet-induced hepatic steatosis	358:400	high-sucrose diet-induced hepatic steatosis	358:400	Herein, inulin consumption effectively ameliorated high-sucrose diet-induced hepatic steatosis and inflammation, and rehabilitated liver lipogenesis regulators, including carbohydrate response element-binding protein, stearoyl-CoA desaturase-1 and peroxisome proliferator-activated receptor alpha.					
36596207	4	88	theme	fatty	940:944	arg1	SCFA					952:955	SCFA	952:955	SCFA	952:955	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	4	88	theme	fatty	940:944	arg1	acid					946:949	short-chain fatty acid	928:949	short-chain fatty acid (SCFA)-producers	928:966	High-throughput sequencing demonstrated that inulin administration regulated the gut microbiota composition, wherein abundance of short-chain fatty acid (SCFA)-producers, including Bifidobacterium, Phascolarctobacterium and Blautia, was significantly enhanced in the inulin-treated rats, conversely, opportunistic pathogens, such as Acinetobacter and Corynebacterium_1, were suppressed.					
36596207	8	89	from	treatment	1986:1994	arg1	future					2008:2013	future	2008:2013	future	2008:2013	Therefore, this study demonstrated that inulin intake alleviated hepatic steatosis likely by regulating the gut microbiota composition and function and restoring the intestinal barrier integrity, which may provide a novel notion for the prevention and treatment of NAFLD in future.					
36596207	3	90	theme	inulin	618:623	arg1	supplementation					625:639	inulin supplementation	618:639	inulin supplementation	618:639	Furthermore, inulin supplementation restored the intestinal barrier integrity and function by up-regulating expressions of tight junction proteins (zonula occludens-1, claudin-1 and occludin).					
35259427	11	0	theme	recipient	1549:1557	arg1	mice					1559:1562	donor and recipient mice	1539:1562	donor and recipient mice	1539:1562	Upon characterization of the microbiota in donor and recipient mice, specific taxa were found to be associated with behavioural changes, notably members of the Lachnospiraceae family.					
35259427	5	1	from	behaviour	753:761	arg1	mice					855:858	male mice	850:858	male mice	850:858	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	8	2	theme	colonic	1148:1154	arg1	markers					1169:1175	colonic inflammatory markers	1148:1175	colonic inflammatory markers	1148:1175	DSS colitis was characterized by a significant reduction in body weight and an increase in colonic inflammatory markers.					
35259427	11	3	theme	behavioural	1612:1622	arg1	changes					1624:1630	behavioural changes	1612:1630	behavioural changes	1612:1630	Upon characterization of the microbiota in donor and recipient mice, specific taxa were found to be associated with behavioural changes, notably members of the Lachnospiraceae family.					
35259427	11	3	theme	behavioural	1612:1622	arg1	members					1641:1647	members	1641:1647	members of the Lachnospiraceae family	1641:1677	Upon characterization of the microbiota in donor and recipient mice, specific taxa were found to be associated with behavioural changes, notably members of the Lachnospiraceae family.					
35259427	4	4	theme	behavioural	626:636	arg1	abnormalities					638:650	behavioural abnormalities	626:650	behavioural abnormalities	626:650	As the intestinal microbiota profoundly influences host behaviour, we sought to determine whether the altered gut microbiota associated with intestinal inflammation contributes to the development of behavioural abnormalities.					
35259427	11	5	theme	donor	1539:1543	arg1	mice					1559:1562	donor and recipient mice	1539:1562	donor and recipient mice	1539:1562	Upon characterization of the microbiota in donor and recipient mice, specific taxa were found to be associated with behavioural changes, notably members of the Lachnospiraceae family.					
35259427	2	6	theme	emotional	276:284	arg1	depression					323:332	depression	323:332	depression	323:332	IBD are associated with a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression.					
35259427	2	6	theme	emotional	276:284	arg1	comorbidities					286:298	cognitive, behavioural and emotional comorbidities	249:298	cognitive, behavioural and emotional comorbidities	249:298	IBD are associated with a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression.					
35259427	2	6	theme	emotional	276:284	arg1	anxiety					311:317	anxiety	311:317	anxiety	311:317	IBD are associated with a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression.					
35259427	5	7	from	inflammation	739:750	arg1	mice					855:858	male mice	850:858	male mice	850:858	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	11	8	theme	Lachnospiraceae	1656:1670	arg1	family					1672:1677	the Lachnospiraceae family	1652:1677	the Lachnospiraceae family	1652:1677	Upon characterization of the microbiota in donor and recipient mice, specific taxa were found to be associated with behavioural changes, notably members of the Lachnospiraceae family.					
35259427	5	9	theme	sulphate	671:678	arg1	sodium					680:685	dextran sulphate sodium	663:685	the dextran sulphate sodium (DSS) model of colitis	659:708	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	9	theme	sulphate	671:678	arg1	DSS					688:690	DSS	688:690	DSS	688:690	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	10	10	theme	recipient	1433:1441	arg1	mice					1443:1446	both GF and Abx-treated recipient mice	1409:1446	mice	1443:1446	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
35259427	2	11	theme	behavioural	260:270	arg1	depression					323:332	depression	323:332	depression	323:332	IBD are associated with a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression.					
35259427	2	11	theme	behavioural	260:270	arg1	comorbidities					286:298	cognitive, behavioural and emotional comorbidities	249:298	cognitive, behavioural and emotional comorbidities	249:298	IBD are associated with a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression.					
35259427	2	11	theme	behavioural	260:270	arg1	anxiety					311:317	anxiety	311:317	anxiety	311:317	IBD are associated with a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression.					
35259427	9	12	theme	increased	1212:1220	arg1	behaviour					1235:1243	increased anxiety-like behaviour	1212:1243	increased anxiety-like behaviour	1212:1243	These changes were accompanied by increased anxiety-like behaviour, an altered gut microbiota composition, and increased central Tnf expression.					
35259427	1	13	theme	gastrointestinal	180:195	arg1	tract					197:201	the gastrointestinal tract	176:201	the gastrointestinal tract	176:201	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	12	14	from	changes	1902:1908	arg1	colitis					1913:1919	colitis	1913:1919	colitis	1913:1919	Behavioural abnormalities associated with intestinal inflammation are transmissible via transfer of cecal matter, suggesting that alterations in the composition of the gut microbiota play a key role in driving behavioural changes in colitis.					
35259427	12	15	theme	cecal	1780:1784	arg1	matter					1786:1791	cecal matter	1780:1791	cecal matter	1780:1791	Behavioural abnormalities associated with intestinal inflammation are transmissible via transfer of cecal matter, suggesting that alterations in the composition of the gut microbiota play a key role in driving behavioural changes in colitis.					
35259427	6	16	theme	-treated	950:957	arg1	mice					959:962	germ-free (GF) or antibiotic (Abx)-treated mice	916:962	mice	959:962	Cecal contents from colitic mice were transferred into germ-free (GF) or antibiotic (Abx)-treated mice, and behaviour was characterized in recipient mice.					
35259427	6	17	theme	Abx	946:948	arg1	mice					959:962	germ-free (GF) or antibiotic (Abx)-treated mice	916:962	mice	959:962	Cecal contents from colitic mice were transferred into germ-free (GF) or antibiotic (Abx)-treated mice, and behaviour was characterized in recipient mice.					
35259427	4	18	theme	host	478:481	arg1	behaviour					483:491	host behaviour	478:491	host behaviour	478:491	As the intestinal microbiota profoundly influences host behaviour, we sought to determine whether the altered gut microbiota associated with intestinal inflammation contributes to the development of behavioural abnormalities.					
35259427	9	19	theme	gut	1257:1259	arg1	composition					1272:1282	an altered gut microbiota composition	1246:1282	an altered gut microbiota composition	1246:1282	These changes were accompanied by increased anxiety-like behaviour, an altered gut microbiota composition, and increased central Tnf expression.					
35259427	6	20	theme	antibiotic	934:943	arg1	mice					959:962	germ-free (GF) or antibiotic (Abx)-treated mice	916:962	mice	959:962	Cecal contents from colitic mice were transferred into germ-free (GF) or antibiotic (Abx)-treated mice, and behaviour was characterized in recipient mice.					
35259427	2	21	theme	cognitive	249:257	arg1	depression					323:332	depression	323:332	depression	323:332	IBD are associated with a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression.					
35259427	2	21	theme	cognitive	249:257	arg1	comorbidities					286:298	cognitive, behavioural and emotional comorbidities	249:298	cognitive, behavioural and emotional comorbidities	249:298	IBD are associated with a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression.					
35259427	2	21	theme	cognitive	249:257	arg1	anxiety					311:317	anxiety	311:317	anxiety	311:317	IBD are associated with a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression.					
35259427	4	22	theme	intestinal	434:443	arg1	microbiota					445:454	the intestinal microbiota	430:454	the intestinal microbiota	430:454	As the intestinal microbiota profoundly influences host behaviour, we sought to determine whether the altered gut microbiota associated with intestinal inflammation contributes to the development of behavioural abnormalities.					
35259427	5	23	theme	tail	787:790	arg1	suspension					792:801	tail suspension	787:801	tail suspension	787:801	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	6	24	theme	recipient	1000:1008	arg1	mice					1010:1013	recipient mice	1000:1013	recipient mice	1000:1013	Cecal contents from colitic mice were transferred into germ-free (GF) or antibiotic (Abx)-treated mice, and behaviour was characterized in recipient mice.					
35259427	9	25	theme	central	1299:1305	arg1	expression					1311:1320	increased central Tnf expression	1289:1320	increased central Tnf expression	1289:1320	These changes were accompanied by increased anxiety-like behaviour, an altered gut microbiota composition, and increased central Tnf expression.					
35259427	1	26	theme	inflammatory	149:160	arg1	conditions					162:171	chronic inflammatory conditions	141:171	chronic inflammatory conditions of the gastrointestinal tract	141:201	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	1	26	theme	inflammatory	149:160	arg1	diseases					122:129	Inflammatory bowel diseases	103:129	Inflammatory bowel diseases (IBD)	103:135	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	12	27	theme	intestinal	1722:1731	arg1	inflammation					1733:1744	intestinal inflammation	1722:1744	intestinal inflammation	1722:1744	Behavioural abnormalities associated with intestinal inflammation are transmissible via transfer of cecal matter, suggesting that alterations in the composition of the gut microbiota play a key role in driving behavioural changes in colitis.					
35259427	11	28	from	characterization	1501:1516	arg1	mice					1559:1562	donor and recipient mice	1539:1562	donor and recipient mice	1539:1562	Upon characterization of the microbiota in donor and recipient mice, specific taxa were found to be associated with behavioural changes, notably members of the Lachnospiraceae family.					
35259427	5	29	theme	elevated	764:771	arg1	behaviour					753:761	behaviour	753:761	behaviour (elevated plus maze and tail suspension test)	753:807	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	29	theme	elevated	764:771	arg1	test					803:806	elevated plus maze and tail suspension test	764:806	elevated plus maze and tail suspension test	764:806	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	30	from	microbiota	836:845	arg1	mice					855:858	male mice	850:858	male mice	850:858	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	10	31	theme	or	1474:1475	arg1	neuroinflammation					1477:1493	colonic or neuroinflammation	1466:1493	colonic or neuroinflammation	1466:1493	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
35259427	5	32	theme	maze	778:781	arg1	behaviour					753:761	behaviour	753:761	behaviour (elevated plus maze and tail suspension test)	753:807	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	32	theme	maze	778:781	arg1	test					803:806	elevated plus maze and tail suspension test	764:806	elevated plus maze and tail suspension test	764:806	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	12	33	theme	Behavioural	1680:1690	arg1	abnormalities					1692:1704	Behavioural abnormalities	1680:1704	Behavioural abnormalities associated with intestinal inflammation	1680:1744	Behavioural abnormalities associated with intestinal inflammation are transmissible via transfer of cecal matter, suggesting that alterations in the composition of the gut microbiota play a key role in driving behavioural changes in colitis.					
35259427	3	34	theme	behavioural	379:389	arg1	comorbidities					391:403	behavioural comorbidities	379:403	behavioural comorbidities	379:403	The link between IBD and the development of behavioural comorbidities is poorly understood.					
35259427	0	35	theme	Colitis-associated	0:17	arg1	microbiota					19:28	Colitis-associated microbiota	0:28	Colitis-associated microbiota	0:28	Colitis-associated microbiota drives changes in behaviour in male mice in the absence of inflammation.					
35259427	8	36	theme	DSS	1057:1059	arg1	colitis					1061:1067	DSS colitis	1057:1067	DSS colitis	1057:1067	DSS colitis was characterized by a significant reduction in body weight and an increase in colonic inflammatory markers.					
35259427	4	37	theme	intestinal	568:577	arg1	inflammation					579:590	intestinal inflammation	568:590	intestinal inflammation	568:590	As the intestinal microbiota profoundly influences host behaviour, we sought to determine whether the altered gut microbiota associated with intestinal inflammation contributes to the development of behavioural abnormalities.					
35259427	8	38	from	increase	1136:1143	arg1	weight					1122:1127	body weight	1117:1127	body weight	1117:1127	DSS colitis was characterized by a significant reduction in body weight and an increase in colonic inflammatory markers.					
35259427	8	38	from	increase	1136:1143	arg1	markers					1169:1175	colonic inflammatory markers	1148:1175	colonic inflammatory markers	1148:1175	DSS colitis was characterized by a significant reduction in body weight and an increase in colonic inflammatory markers.					
35259427	1	39	theme	Inflammatory	103:114	arg1	conditions					162:171	chronic inflammatory conditions	141:171	chronic inflammatory conditions of the gastrointestinal tract	141:201	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	1	39	theme	Inflammatory	103:114	arg1	IBD					132:134	IBD	132:134	IBD	132:134	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	1	39	theme	Inflammatory	103:114	arg1	diseases					122:129	Inflammatory bowel diseases	103:129	Inflammatory bowel diseases (IBD)	103:135	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	12	40	theme	gut	1848:1850	arg1	microbiota					1852:1861	the gut microbiota	1844:1861	the gut microbiota	1844:1861	Behavioural abnormalities associated with intestinal inflammation are transmissible via transfer of cecal matter, suggesting that alterations in the composition of the gut microbiota play a key role in driving behavioural changes in colitis.					
35259427	4	41	theme	gut	537:539	arg1	microbiota					541:550	the altered gut microbiota	525:550	the altered gut microbiota associated with intestinal inflammation	525:590	As the intestinal microbiota profoundly influences host behaviour, we sought to determine whether the altered gut microbiota associated with intestinal inflammation contributes to the development of behavioural abnormalities.					
35259427	10	42	theme	matter	1345:1350	arg1	Transfer					1323:1330	Transfer	1323:1330	Transfer of the cecal matter from colitic mice	1323:1368	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
35259427	8	43	theme	significant	1092:1102	arg1	reduction					1104:1112	a significant reduction	1090:1112	a significant reduction in body weight	1090:1127	DSS colitis was characterized by a significant reduction in body weight and an increase in colonic inflammatory markers.					
35259427	6	44	theme	Cecal	861:865	arg1	contents					867:874	Cecal contents	861:874	Cecal contents from colitic mice	861:892	Cecal contents from colitic mice were transferred into germ-free (GF) or antibiotic (Abx)-treated mice, and behaviour was characterized in recipient mice.					
35259427	4	45	theme	abnormalities	638:650	arg1	development					611:621	the development	607:621	the development of behavioural abnormalities	607:650	As the intestinal microbiota profoundly influences host behaviour, we sought to determine whether the altered gut microbiota associated with intestinal inflammation contributes to the development of behavioural abnormalities.					
35259427	10	46	theme	colitic	1357:1363	arg1	mice					1365:1368	colitic mice	1357:1368	colitic mice	1357:1368	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
35259427	9	47	theme	increased	1289:1297	arg1	expression					1311:1320	increased central Tnf expression	1289:1320	increased central Tnf expression	1289:1320	These changes were accompanied by increased anxiety-like behaviour, an altered gut microbiota composition, and increased central Tnf expression.					
35259427	2	48	theme	comorbidities	286:298	arg1	prevalence					235:244	a high prevalence	228:244	a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression	228:332	IBD are associated with a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression.					
35259427	6	49	theme	germ-free	916:924	arg1	GF					927:928	germ-free (GF) or antibiotic (Abx)-treated mice	916:962	GF	927:928	Cecal contents from colitic mice were transferred into germ-free (GF) or antibiotic (Abx)-treated mice, and behaviour was characterized in recipient mice.					
35259427	5	50	theme	dextran	663:669	arg1	sodium					680:685	dextran sulphate sodium	663:685	the dextran sulphate sodium (DSS) model of colitis	659:708	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	50	theme	dextran	663:669	arg1	DSS					688:690	DSS	688:690	DSS	688:690	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	10	51	theme	behavioural	1386:1396	arg1	changes					1398:1404	similar behavioural changes	1378:1404	similar behavioural changes in both GF and Abx-treated recipient mice	1378:1446	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
35259427	8	52	theme	inflammatory	1156:1167	arg1	markers					1169:1175	colonic inflammatory markers	1148:1175	colonic inflammatory markers	1148:1175	DSS colitis was characterized by a significant reduction in body weight and an increase in colonic inflammatory markers.					
35259427	11	53	theme	family	1672:1677	arg1	changes					1624:1630	behavioural changes	1612:1630	behavioural changes	1612:1630	Upon characterization of the microbiota in donor and recipient mice, specific taxa were found to be associated with behavioural changes, notably members of the Lachnospiraceae family.					
35259427	11	53	theme	family	1672:1677	arg1	members					1641:1647	members	1641:1647	members of the Lachnospiraceae family	1641:1677	Upon characterization of the microbiota in donor and recipient mice, specific taxa were found to be associated with behavioural changes, notably members of the Lachnospiraceae family.					
35259427	5	54	theme	sodium	680:685	arg1	model					693:697	the dextran sulphate sodium (DSS) model	659:697	the dextran sulphate sodium (DSS) model of colitis	659:708	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	10	55	theme	Abx-treated	1421:1431	arg1	mice					1443:1446	both GF and Abx-treated recipient mice	1409:1446	mice	1443:1446	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
35259427	12	56	theme	matter	1786:1791	arg1	transfer					1768:1775	transfer	1768:1775	transfer of cecal matter	1768:1791	Behavioural abnormalities associated with intestinal inflammation are transmissible via transfer of cecal matter, suggesting that alterations in the composition of the gut microbiota play a key role in driving behavioural changes in colitis.					
35259427	6	57	from	mice	889:892	arg1	contents					867:874	Cecal contents	861:874	Cecal contents from colitic mice	861:892	Cecal contents from colitic mice were transferred into germ-free (GF) or antibiotic (Abx)-treated mice, and behaviour was characterized in recipient mice.					
35259427	5	58	theme	intestinal	728:737	arg1	inflammation					739:750	intestinal inflammation	728:750	intestinal inflammation	728:750	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	9	59	theme	anxiety-like	1222:1233	arg1	behaviour					1235:1243	increased anxiety-like behaviour	1212:1243	increased anxiety-like behaviour	1212:1243	These changes were accompanied by increased anxiety-like behaviour, an altered gut microbiota composition, and increased central Tnf expression.					
35259427	1	60	theme	tract	197:201	arg1	conditions					162:171	chronic inflammatory conditions	141:171	chronic inflammatory conditions of the gastrointestinal tract	141:201	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	1	60	theme	tract	197:201	arg1	diseases					122:129	Inflammatory bowel diseases	103:129	Inflammatory bowel diseases (IBD)	103:135	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	11	61	from	mice	1559:1562	arg1	characterization					1501:1516	characterization	1501:1516	characterization of the microbiota in donor and recipient mice	1501:1562	Upon characterization of the microbiota in donor and recipient mice, specific taxa were found to be associated with behavioural changes, notably members of the Lachnospiraceae family.					
35259427	2	62	theme	high	230:233	arg1	prevalence					235:244	a high prevalence	228:244	a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression	228:332	IBD are associated with a high prevalence of cognitive, behavioural and emotional comorbidities, including anxiety and depression.					
35259427	5	63	theme	colitis	702:708	arg1	model					693:697	the dextran sulphate sodium (DSS) model	659:697	the dextran sulphate sodium (DSS) model of colitis	659:708	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	9	64	theme	altered	1249:1255	arg1	composition					1272:1282	an altered gut microbiota composition	1246:1282	an altered gut microbiota composition	1246:1282	These changes were accompanied by increased anxiety-like behaviour, an altered gut microbiota composition, and increased central Tnf expression.					
35259427	7	65	theme	Gene	1016:1019	arg1	expression					1021:1030	Gene expression	1016:1030	Gene expression	1016:1030	Gene expression was measured using qPCR.					
35259427	1	66	theme	bowel	116:120	arg1	conditions					162:171	chronic inflammatory conditions	141:171	chronic inflammatory conditions of the gastrointestinal tract	141:201	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	1	66	theme	bowel	116:120	arg1	IBD					132:134	IBD	132:134	IBD	132:134	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	1	66	theme	bowel	116:120	arg1	diseases					122:129	Inflammatory bowel diseases	103:129	Inflammatory bowel diseases (IBD)	103:135	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	11	67	theme	specific	1565:1572	arg1	taxa					1574:1577	specific taxa	1565:1577	specific taxa	1565:1577	Upon characterization of the microbiota in donor and recipient mice, specific taxa were found to be associated with behavioural changes, notably members of the Lachnospiraceae family.					
35259427	9	68	theme	microbiota	1261:1270	arg1	composition					1272:1282	an altered gut microbiota composition	1246:1282	an altered gut microbiota composition	1246:1282	These changes were accompanied by increased anxiety-like behaviour, an altered gut microbiota composition, and increased central Tnf expression.					
35259427	5	69	from	mice	855:858	arg1	inflammation					739:750	intestinal inflammation	728:750	intestinal inflammation	728:750	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	69	from	mice	855:858	arg1	test					803:806	elevated plus maze and tail suspension test	764:806	elevated plus maze and tail suspension test	764:806	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	69	from	mice	855:858	arg1	composition					817:827	the composition	813:827	the composition of the microbiota in male mice	813:858	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	69	from	mice	855:858	arg1	behaviour					753:761	behaviour	753:761	behaviour (elevated plus maze and tail suspension test)	753:807	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	70	theme	male	850:853	arg1	mice					855:858	male mice	850:858	male mice	850:858	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	0	71	from	changes	37:43	arg1	behaviour					48:56	behaviour	48:56	behaviour	48:56	Colitis-associated microbiota drives changes in behaviour in male mice in the absence of inflammation.					
35259427	0	71	from	changes	37:43	arg1	mice					66:69	male mice	61:69	male mice	61:69	Colitis-associated microbiota drives changes in behaviour in male mice in the absence of inflammation.					
35259427	11	72	theme	microbiota	1525:1534	arg1	characterization					1501:1516	characterization	1501:1516	characterization of the microbiota in donor and recipient mice	1501:1562	Upon characterization of the microbiota in donor and recipient mice, specific taxa were found to be associated with behavioural changes, notably members of the Lachnospiraceae family.					
35259427	5	73	theme	suspension	792:801	arg1	behaviour					753:761	behaviour	753:761	behaviour (elevated plus maze and tail suspension test)	753:807	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	73	theme	suspension	792:801	arg1	test					803:806	elevated plus maze and tail suspension test	764:806	elevated plus maze and tail suspension test	764:806	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	74	from	composition	817:827	arg1	mice					855:858	male mice	850:858	male mice	850:858	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	1	75	theme	chronic	141:147	arg1	conditions					162:171	chronic inflammatory conditions	141:171	chronic inflammatory conditions of the gastrointestinal tract	141:201	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	1	75	theme	chronic	141:147	arg1	diseases					122:129	Inflammatory bowel diseases	103:129	Inflammatory bowel diseases (IBD)	103:135	Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract.					
35259427	4	76	theme	altered	529:535	arg1	microbiota					541:550	the altered gut microbiota	525:550	the altered gut microbiota associated with intestinal inflammation	525:590	As the intestinal microbiota profoundly influences host behaviour, we sought to determine whether the altered gut microbiota associated with intestinal inflammation contributes to the development of behavioural abnormalities.					
35259427	0	77	theme	inflammation	89:100	arg1	absence					78:84	the absence	74:84	the absence of inflammation	74:100	Colitis-associated microbiota drives changes in behaviour in male mice in the absence of inflammation.					
35259427	9	78	theme	Tnf	1307:1309	arg1	expression					1311:1320	increased central Tnf expression	1289:1320	increased central Tnf expression	1289:1320	These changes were accompanied by increased anxiety-like behaviour, an altered gut microbiota composition, and increased central Tnf expression.					
35259427	8	79	from	reduction	1104:1112	arg1	weight					1122:1127	body weight	1117:1127	body weight	1117:1127	DSS colitis was characterized by a significant reduction in body weight and an increase in colonic inflammatory markers.					
35259427	8	79	from	reduction	1104:1112	arg1	markers					1169:1175	colonic inflammatory markers	1148:1175	colonic inflammatory markers	1148:1175	DSS colitis was characterized by a significant reduction in body weight and an increase in colonic inflammatory markers.					
35259427	11	80	from	microbiota	1525:1534	arg1	mice					1559:1562	donor and recipient mice	1539:1562	donor and recipient mice	1539:1562	Upon characterization of the microbiota in donor and recipient mice, specific taxa were found to be associated with behavioural changes, notably members of the Lachnospiraceae family.					
35259427	12	81	from	alterations	1810:1820	arg1	composition					1829:1839	the composition	1825:1839	the composition of the gut microbiota	1825:1861	Behavioural abnormalities associated with intestinal inflammation are transmissible via transfer of cecal matter, suggesting that alterations in the composition of the gut microbiota play a key role in driving behavioural changes in colitis.					
35259427	10	82	theme	colonic	1466:1472	arg1	neuroinflammation					1477:1493	colonic or neuroinflammation	1466:1493	colonic or neuroinflammation	1466:1493	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
35259427	3	83	theme	comorbidities	391:403	arg1	IBD					352:354	IBD	352:354	IBD	352:354	The link between IBD and the development of behavioural comorbidities is poorly understood.					
35259427	3	83	theme	comorbidities	391:403	arg1	development					364:374	the development	360:374	the development of behavioural comorbidities	360:403	The link between IBD and the development of behavioural comorbidities is poorly understood.					
35259427	5	84	theme	microbiota	836:845	arg1	inflammation					739:750	intestinal inflammation	728:750	intestinal inflammation	728:750	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	84	theme	microbiota	836:845	arg1	test					803:806	elevated plus maze and tail suspension test	764:806	elevated plus maze and tail suspension test	764:806	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	84	theme	microbiota	836:845	arg1	composition					817:827	the composition	813:827	the composition of the microbiota in male mice	813:858	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	5	84	theme	microbiota	836:845	arg1	behaviour					753:761	behaviour	753:761	behaviour (elevated plus maze and tail suspension test)	753:807	Using the dextran sulphate sodium (DSS) model of colitis, we characterized intestinal inflammation, behaviour (elevated plus maze and tail suspension test) and the composition of the microbiota in male mice.					
35259427	12	85	theme	behavioural	1890:1900	arg1	changes					1902:1908	behavioural changes	1890:1908	behavioural changes in colitis	1890:1919	Behavioural abnormalities associated with intestinal inflammation are transmissible via transfer of cecal matter, suggesting that alterations in the composition of the gut microbiota play a key role in driving behavioural changes in colitis.					
35259427	10	86	from	mice	1365:1368	arg1	Transfer					1323:1330	Transfer	1323:1330	Transfer of the cecal matter from colitic mice	1323:1368	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
35259427	6	87	theme	colitic	881:887	arg1	mice					889:892	colitic mice	881:892	colitic mice	881:892	Cecal contents from colitic mice were transferred into germ-free (GF) or antibiotic (Abx)-treated mice, and behaviour was characterized in recipient mice.					
35259427	10	88	theme	neuroinflammation	1477:1493	arg1	signs					1457:1461	no signs	1454:1461	no signs of colonic or neuroinflammation	1454:1493	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
35259427	0	89	theme	male	61:64	arg1	mice					66:69	male mice	61:69	male mice	61:69	Colitis-associated microbiota drives changes in behaviour in male mice in the absence of inflammation.					
35259427	12	90	theme	key	1870:1872	arg1	role					1874:1877	a key role	1868:1877	a key role	1868:1877	Behavioural abnormalities associated with intestinal inflammation are transmissible via transfer of cecal matter, suggesting that alterations in the composition of the gut microbiota play a key role in driving behavioural changes in colitis.					
35259427	10	91	from	changes	1398:1404	arg1	mice					1443:1446	both GF and Abx-treated recipient mice	1409:1446	mice	1443:1446	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
35259427	10	91	from	changes	1398:1404	arg1	GF					1414:1415	both GF and Abx-treated recipient mice	1409:1446	GF	1414:1415	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
35259427	10	92	theme	cecal	1339:1343	arg1	matter					1345:1350	the cecal matter	1335:1350	the cecal matter	1335:1350	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
35259427	8	93	theme	body	1117:1120	arg1	weight					1122:1127	body weight	1117:1127	body weight	1117:1127	DSS colitis was characterized by a significant reduction in body weight and an increase in colonic inflammatory markers.					
35259427	12	94	theme	microbiota	1852:1861	arg1	composition					1829:1839	the composition	1825:1839	the composition of the gut microbiota	1825:1861	Behavioural abnormalities associated with intestinal inflammation are transmissible via transfer of cecal matter, suggesting that alterations in the composition of the gut microbiota play a key role in driving behavioural changes in colitis.					
35259427	10	95	theme	similar	1378:1384	arg1	changes					1398:1404	similar behavioural changes	1378:1404	similar behavioural changes in both GF and Abx-treated recipient mice	1378:1446	Transfer of the cecal matter from colitic mice induced similar behavioural changes in both GF and Abx-treated recipient mice, with no signs of colonic or neuroinflammation.					
37364963	1	0	theme	mechanism	147:155	arg1	effect					119:124	The effect	115:124	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes	115:232	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37364963	2	1	theme	heated-induced	366:379	arg1	cross-linking					381:393	heated-induced cross-linking	366:393	heated-induced cross-linking	366:393	The results showed that the binding effecting and spatial network structure of 11S-PS complexes via heated-induced cross-linking were adjusted by biopolymer ratios.					
37364963	3	2	contain	had	499:501	arg2	interaction					530:540	a strongest intermolecular interaction	503:540	a strongest intermolecular interaction through hydrogen bonds	503:563	In particular, 11S-PS complexes with the biopolymer ratios of 2:15, had a strongest intermolecular interaction through hydrogen bonds and hydrophobic force.					
37364963	3	2	contain	had	499:501	arg2	force					581:585	hydrophobic force	569:585	hydrophobic force	569:585	In particular, 11S-PS complexes with the biopolymer ratios of 2:15, had a strongest intermolecular interaction through hydrogen bonds and hydrophobic force.					
37364963	3	2	contain	had	499:501	arg1	complexes					453:461	11S-PS complexes	446:461	11S-PS complexes	446:461	In particular, 11S-PS complexes with the biopolymer ratios of 2:15, had a strongest intermolecular interaction through hydrogen bonds and hydrophobic force.					
37364963	4	3	used	used	713:716	arg2	solution					734:741	film-forming solution	721:741	film-forming solution	721:741	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	4	3	used	used	713:716	arg2	structure					692:700	a finer three-dimensional network structure	658:700	a finer three-dimensional network structure	658:700	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	2	4	theme	spatial	316:322	arg1	structure					332:340	spatial network structure	316:340	spatial network structure	316:340	The results showed that the binding effecting and spatial network structure of 11S-PS complexes via heated-induced cross-linking were adjusted by biopolymer ratios.					
37364963	1	5	theme	complexes	224:232	arg1	mechanism					147:155	cross-linking mechanism	133:155	cross-linking mechanism	133:155	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37364963	1	5	theme	complexes	224:232	arg1	properties					172:181	functional properties	161:181	functional properties	161:181	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37364963	3	6	theme	intermolecular	515:528	arg1	interaction					530:540	a strongest intermolecular interaction	503:540	a strongest intermolecular interaction through hydrogen bonds	503:563	In particular, 11S-PS complexes with the biopolymer ratios of 2:15, had a strongest intermolecular interaction through hydrogen bonds and hydrophobic force.					
37364963	3	7	theme	hydrogen	550:557	arg1	bonds					559:563	hydrogen bonds	550:563	hydrogen bonds	550:563	In particular, 11S-PS complexes with the biopolymer ratios of 2:15, had a strongest intermolecular interaction through hydrogen bonds and hydrophobic force.					
37364963	6	8	theme	food	1170:1173	arg1	preservation					1175:1186	food preservation	1170:1186	food preservation	1170:1186	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	5	9	theme	truss	957:961	arg1	experiments					983:993	truss tomato preservation experiments	957:993	truss tomato preservation experiments	957:993	In addition, the 11S-PS complexes coating was effective in moderating the loss of nutrients, thereby extending their storage life in truss tomato preservation experiments.					
37364963	2	10	theme	network	324:330	arg1	structure					332:340	spatial network structure	316:340	spatial network structure	316:340	The results showed that the binding effecting and spatial network structure of 11S-PS complexes via heated-induced cross-linking were adjusted by biopolymer ratios.					
37364963	5	11	theme	complexes	848:856	arg1	coating					858:864	the 11S-PS complexes coating	837:864	the 11S-PS complexes coating	837:864	In addition, the 11S-PS complexes coating was effective in moderating the loss of nutrients, thereby extending their storage life in truss tomato preservation experiments.					
37364963	5	11	theme	complexes	848:856	arg1	effective					870:878	effective	870:878	effective	870:878	In addition, the 11S-PS complexes coating was effective in moderating the loss of nutrients, thereby extending their storage life in truss tomato preservation experiments.					
37364963	6	12	theme	composite	1148:1156	arg1	coatings					1158:1165	food-grade biopolymer composite coatings	1126:1165	food-grade biopolymer composite coatings	1126:1165	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	6	13	theme	complexes	1083:1091	arg1	mechanism					1059:1067	the cross-linking mechanism	1041:1067	the cross-linking mechanism of the 11S-PS complexes	1041:1091	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	6	13	theme	complexes	1083:1091	arg1	application					1111:1121	the potential application	1097:1121	the potential application of food-grade biopolymer composite coatings in food preservation	1097:1186	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	5	14	theme	tomato	963:968	arg1	experiments					983:993	truss tomato preservation experiments	957:993	truss tomato preservation experiments	957:993	In addition, the 11S-PS complexes coating was effective in moderating the loss of nutrients, thereby extending their storage life in truss tomato preservation experiments.					
37364963	4	15	theme	finer	660:664	arg1	structure					692:700	a finer three-dimensional network structure	658:700	a finer three-dimensional network structure	658:700	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	4	15	theme	finer	660:664	arg1	solution					734:741	film-forming solution	721:741	film-forming solution	721:741	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	1	16	theme	functional	161:170	arg1	properties					172:181	functional properties	161:181	functional properties	161:181	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37364963	5	17	from	effective	870:878	arg1	addition					827:834	addition	827:834	addition	827:834	In addition, the 11S-PS complexes coating was effective in moderating the loss of nutrients, thereby extending their storage life in truss tomato preservation experiments.					
37364963	3	18	theme	strongest	505:513	arg1	interaction					530:540	a strongest intermolecular interaction	503:540	a strongest intermolecular interaction through hydrogen bonds	503:563	In particular, 11S-PS complexes with the biopolymer ratios of 2:15, had a strongest intermolecular interaction through hydrogen bonds and hydrophobic force.					
37364963	1	19	theme	properties	172:181	arg1	effect					119:124	The effect	115:124	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes	115:232	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37364963	1	20	dep	mechanism	147:155	arg1	the					129:131	the	129:131	the	129:131	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37364963	2	21	theme	complexes	352:360	arg1	effecting					302:310	binding effecting	294:310	binding effecting	294:310	The results showed that the binding effecting and spatial network structure of 11S-PS complexes via heated-induced cross-linking were adjusted by biopolymer ratios.					
37364963	2	21	theme	complexes	352:360	arg1	structure					332:340	spatial network structure	316:340	spatial network structure	316:340	The results showed that the binding effecting and spatial network structure of 11S-PS complexes via heated-induced cross-linking were adjusted by biopolymer ratios.					
37364963	0	22	theme	Biomacromolecule	0:15	arg1	assembly					17:24	Biomacromolecule assembly	0:24	Biomacromolecule assembly of soy glycinin-potato starch complexes: Focus on structure, function, and applications.	0:113	Biomacromolecule assembly of soy glycinin-potato starch complexes: Focus on structure, function, and applications.					
37364963	6	23	from	mechanism	1059:1067	arg1	preservation					1175:1186	food preservation	1170:1186	food preservation	1170:1186	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	4	24	theme	film-forming	721:732	arg1	structure					692:700	a finer three-dimensional network structure	658:700	a finer three-dimensional network structure	658:700	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	4	24	theme	film-forming	721:732	arg1	solution					734:741	film-forming solution	721:741	film-forming solution	721:741	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	1	25	theme	soy	186:188	arg1	complexes					224:232	soy glycinin (11S)-potato starch (PS) complexes	186:232	soy glycinin (11S)-potato starch (PS) complexes	186:232	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37364963	0	26	theme	soy	29:31	arg1	complexes					56:64	soy glycinin-potato starch complexes	29:64	soy glycinin-potato starch complexes	29:64	Biomacromolecule assembly of soy glycinin-potato starch complexes: Focus on structure, function, and applications.					
37364963	6	27	theme	11S-PS	1076:1081	arg1	complexes					1083:1091	the 11S-PS complexes	1072:1091	the 11S-PS complexes	1072:1091	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	5	28	theme	storage	941:947	arg1	life					949:952	their storage life	935:952	their storage life	935:952	In addition, the 11S-PS complexes coating was effective in moderating the loss of nutrients, thereby extending their storage life in truss tomato preservation experiments.					
37364963	4	29	from	ratios	633:638	arg1	complexes					605:613	11S-PS complexes	598:613	11S-PS complexes at the biopolymer ratios of 2:15	598:646	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	1	30	theme	glycinin	190:197	arg1	complexes					224:232	soy glycinin (11S)-potato starch (PS) complexes	186:232	soy glycinin (11S)-potato starch (PS) complexes	186:232	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37364963	2	31	theme	biopolymer	412:421	arg1	ratios					423:428	biopolymer ratios	412:428	biopolymer ratios	412:428	The results showed that the binding effecting and spatial network structure of 11S-PS complexes via heated-induced cross-linking were adjusted by biopolymer ratios.					
37364963	4	32	theme	11S-PS	598:603	arg1	complexes					605:613	11S-PS complexes	598:613	11S-PS complexes at the biopolymer ratios of 2:15	598:646	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	2	33	dep	effecting	302:310	arg1	the					290:292	the	290:292	the	290:292	The results showed that the binding effecting and spatial network structure of 11S-PS complexes via heated-induced cross-linking were adjusted by biopolymer ratios.					
37364963	6	34	theme	cross-linking	1045:1057	arg1	mechanism					1059:1067	the cross-linking mechanism	1041:1067	the cross-linking mechanism of the 11S-PS complexes	1041:1091	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	0	35	theme	starch	49:54	arg1	complexes					56:64	soy glycinin-potato starch complexes	29:64	soy glycinin-potato starch complexes	29:64	Biomacromolecule assembly of soy glycinin-potato starch complexes: Focus on structure, function, and applications.					
37364963	6	36	theme	biopolymer	1137:1146	arg1	coatings					1158:1165	food-grade biopolymer composite coatings	1126:1165	food-grade biopolymer composite coatings	1126:1165	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	2	37	theme	11S-PS	345:350	arg1	complexes					352:360	11S-PS complexes	345:360	11S-PS complexes	345:360	The results showed that the binding effecting and spatial network structure of 11S-PS complexes via heated-induced cross-linking were adjusted by biopolymer ratios.					
37364963	1	38	theme	11S	200:202	arg1	complexes					224:232	soy glycinin (11S)-potato starch (PS) complexes	186:232	soy glycinin (11S)-potato starch (PS) complexes	186:232	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37364963	0	39	theme	glycinin-potato	33:47	arg1	complexes					56:64	soy glycinin-potato starch complexes	29:64	soy glycinin-potato starch complexes	29:64	Biomacromolecule assembly of soy glycinin-potato starch complexes: Focus on structure, function, and applications.					
37364963	3	40	with	complexes	453:461	arg1	ratios					483:488	the biopolymer ratios	468:488	the biopolymer ratios of 2:15	468:496	In particular, 11S-PS complexes with the biopolymer ratios of 2:15, had a strongest intermolecular interaction through hydrogen bonds and hydrophobic force.					
37364963	5	41	theme	11S-PS	841:846	arg1	coating					858:864	the 11S-PS complexes coating	837:864	the 11S-PS complexes coating	837:864	In addition, the 11S-PS complexes coating was effective in moderating the loss of nutrients, thereby extending their storage life in truss tomato preservation experiments.					
37364963	5	41	theme	11S-PS	841:846	arg1	effective					870:878	effective	870:878	effective	870:878	In addition, the 11S-PS complexes coating was effective in moderating the loss of nutrients, thereby extending their storage life in truss tomato preservation experiments.					
37364963	0	42	from	Focus	67:71	arg1	structure					76:84	structure	76:84	structure	76:84	Biomacromolecule assembly of soy glycinin-potato starch complexes: Focus on structure, function, and applications.					
37364963	0	42	from	Focus	67:71	arg1	function					87:94	function	87:94	function	87:94	Biomacromolecule assembly of soy glycinin-potato starch complexes: Focus on structure, function, and applications.					
37364963	0	42	from	Focus	67:71	arg1	applications					101:112	applications	101:112	applications	101:112	Biomacromolecule assembly of soy glycinin-potato starch complexes: Focus on structure, function, and applications.					
37364963	3	43	theme	hydrophobic	569:579	arg1	force					581:585	hydrophobic force	569:585	hydrophobic force	569:585	In particular, 11S-PS complexes with the biopolymer ratios of 2:15, had a strongest intermolecular interaction through hydrogen bonds and hydrophobic force.					
37364963	6	44	theme	food-grade	1126:1135	arg1	coatings					1158:1165	food-grade biopolymer composite coatings	1126:1165	food-grade biopolymer composite coatings	1126:1165	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	3	45	theme	biopolymer	472:481	arg1	ratios					483:488	the biopolymer ratios	468:488	the biopolymer ratios of 2:15	468:496	In particular, 11S-PS complexes with the biopolymer ratios of 2:15, had a strongest intermolecular interaction through hydrogen bonds and hydrophobic force.					
37364963	0	46	theme	complexes	56:64	arg1	assembly					17:24	Biomacromolecule assembly	0:24	Biomacromolecule assembly of soy glycinin-potato starch complexes: Focus on structure, function, and applications.	0:113	Biomacromolecule assembly of soy glycinin-potato starch complexes: Focus on structure, function, and applications.					
37364963	3	47	theme	2:15	493:496	arg1	ratios					483:488	the biopolymer ratios	468:488	the biopolymer ratios of 2:15	468:496	In particular, 11S-PS complexes with the biopolymer ratios of 2:15, had a strongest intermolecular interaction through hydrogen bonds and hydrophobic force.					
37364963	5	48	theme	nutrients	906:914	arg1	loss					898:901	the loss	894:901	the loss of nutrients	894:914	In addition, the 11S-PS complexes coating was effective in moderating the loss of nutrients, thereby extending their storage life in truss tomato preservation experiments.					
37364963	2	49	theme	binding	294:300	arg1	effecting					302:310	binding effecting	294:310	binding effecting	294:310	The results showed that the binding effecting and spatial network structure of 11S-PS complexes via heated-induced cross-linking were adjusted by biopolymer ratios.					
37364963	5	50	from	addition	827:834	arg1	coating					858:864	the 11S-PS complexes coating	837:864	the 11S-PS complexes coating	837:864	In addition, the 11S-PS complexes coating was effective in moderating the loss of nutrients, thereby extending their storage life in truss tomato preservation experiments.					
37364963	5	50	from	addition	827:834	arg1	effective					870:878	effective	870:878	effective	870:878	In addition, the 11S-PS complexes coating was effective in moderating the loss of nutrients, thereby extending their storage life in truss tomato preservation experiments.					
37364963	1	51	theme	-potato	204:210	arg1	complexes					224:232	soy glycinin (11S)-potato starch (PS) complexes	186:232	soy glycinin (11S)-potato starch (PS) complexes	186:232	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37364963	5	52	theme	preservation	970:981	arg1	experiments					983:993	truss tomato preservation experiments	957:993	truss tomato preservation experiments	957:993	In addition, the 11S-PS complexes coating was effective in moderating the loss of nutrients, thereby extending their storage life in truss tomato preservation experiments.					
37364963	4	53	theme	barrier	758:764	arg1	performance					766:776	the barrier performance	754:776	the barrier performance	754:776	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	4	54	theme	three-dimensional	666:682	arg1	structure					692:700	a finer three-dimensional network structure	658:700	a finer three-dimensional network structure	658:700	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	4	54	theme	three-dimensional	666:682	arg1	solution					734:741	film-forming solution	721:741	film-forming solution	721:741	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	1	55	theme	starch	212:217	arg1	complexes					224:232	soy glycinin (11S)-potato starch (PS) complexes	186:232	soy glycinin (11S)-potato starch (PS) complexes	186:232	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37364963	6	56	theme	potential	1101:1109	arg1	application					1111:1121	the potential application	1097:1121	the potential application of food-grade biopolymer composite coatings in food preservation	1097:1186	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	0	57	dep	assembly	17:24	arg1	Focus					67:71	Focus	67:71	Biomacromolecule assembly of soy glycinin-potato starch complexes: Focus on structure, function, and applications.	0:113	Biomacromolecule assembly of soy glycinin-potato starch complexes: Focus on structure, function, and applications.					
37364963	3	58	theme	11S-PS	446:451	arg1	complexes					453:461	11S-PS complexes	446:461	11S-PS complexes	446:461	In particular, 11S-PS complexes with the biopolymer ratios of 2:15, had a strongest intermolecular interaction through hydrogen bonds and hydrophobic force.					
37364963	4	59	theme	network	684:690	arg1	structure					692:700	a finer three-dimensional network structure	658:700	a finer three-dimensional network structure	658:700	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	4	59	theme	network	684:690	arg1	solution					734:741	film-forming solution	721:741	film-forming solution	721:741	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	4	60	theme	2:15	643:646	arg1	ratios					633:638	the biopolymer ratios	618:638	the biopolymer ratios of 2:15	618:646	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	6	61	theme	coatings	1158:1165	arg1	mechanism					1059:1067	the cross-linking mechanism	1041:1067	the cross-linking mechanism of the 11S-PS complexes	1041:1091	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	6	61	theme	coatings	1158:1165	arg1	application					1111:1121	the potential application	1097:1121	the potential application of food-grade biopolymer composite coatings in food preservation	1097:1186	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	4	62	theme	biopolymer	622:631	arg1	ratios					633:638	the biopolymer ratios	618:638	the biopolymer ratios of 2:15	618:646	Moreover, 11S-PS complexes at the biopolymer ratios of 2:15 exhibited a finer three-dimensional network structure, which was used as film-forming solution to enhance the barrier performance and mitigate the exposure to the environment.					
37364963	6	63	from	application	1111:1121	arg1	preservation					1175:1186	food preservation	1170:1186	food preservation	1170:1186	This study provides helpful to insights into the cross-linking mechanism of the 11S-PS complexes and the potential application of food-grade biopolymer composite coatings in food preservation.					
37364963	1	64	theme	cross-linking	133:145	arg1	mechanism					147:155	cross-linking mechanism	133:155	cross-linking mechanism	133:155	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37364963	1	65	theme	PS	220:221	arg1	complexes					224:232	soy glycinin (11S)-potato starch (PS) complexes	186:232	soy glycinin (11S)-potato starch (PS) complexes	186:232	The effect of the cross-linking mechanism and functional properties of soy glycinin (11S)-potato starch (PS) complexes was investigated in this study.					
37162339	6	0	theme	abundant	931:938	arg1	genus					950:954	the most abundant bacterial genus	922:954	the most abundant bacterial genus	922:954	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	6	0	theme	abundant	931:938	arg1	Leuconostoc					899:909	Leuconostoc	899:909	Leuconostoc	899:909	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	9	1	theme	sugarcane	1702:1710	arg1	degradation					1718:1728	sugarcane juice degradation	1702:1728	sugarcane juice degradation	1702:1728	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	6	2	theme	microbial	1090:1098	arg1	diversity					1100:1108	the microbial diversity	1086:1108	the microbial diversity present in sugarcane factories	1086:1139	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	8	3	used	utilized	1451:1458	arg2	IMPORTANCE					1410:1419	IMPORTANCE Amplicon-based sequencing	1410:1445	IMPORTANCE Amplicon-based sequencing	1410:1445	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	7	4	theme	consumption	1251:1261	arg1	rate					1235:1238	the rate	1231:1238	the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition	1231:1407	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	4	5	theme	raw	688:690	arg1	factories					698:706	Louisiana raw sugar factories	678:706	Louisiana raw sugar factories	678:706	In the present study, high-throughput amplicon-based sequence profiling was applied to gain a more comprehensive view of the microbial community in Louisiana raw sugar factories.					
37162339	0	6	theme	Microbiome	0:9	arg1	Analysis					11:18	Microbiome Analysis	0:18	Microbiome Analysis of Sugarcane	0:31	Microbiome Analysis of Sugarcane Juices and Biofilms from Louisiana Raw Sugar Factories.					
37162339	8	7	attach	present	1550:1556	arg1	streams					1572:1578	processing streams	1561:1578	processing streams at Louisiana sugarcane raw sugar factories	1561:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	8	7	attach	present	1550:1556	arg2	microbes					1541:1548	microbes	1541:1548	microbes present in processing streams at Louisiana sugarcane raw sugar factories	1541:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	5	8	theme	fungal	880:885	arg1	ASVs					887:890	793 fungal ASVs	876:890	793 fungal ASVs	876:890	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	8	9	theme	raw	1603:1605	arg1	factories					1613:1621	Louisiana sugarcane raw sugar factories	1583:1621	Louisiana sugarcane raw sugar factories	1583:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	6	10	theme	16S	974:976	arg1	sequences					978:986	16S sequences	974:986	16S sequences	974:986	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	9	11	theme	contamination	1763:1775	arg1	problems					1777:1784	microbial contamination problems	1753:1784	microbial contamination problems faced by raw sugar factories	1753:1813	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	4	12	theme	microbial	655:663	arg1	community					665:673	the microbial community	651:673	the microbial community	651:673	In the present study, high-throughput amplicon-based sequence profiling was applied to gain a more comprehensive view of the microbial community in Louisiana raw sugar factories.					
37162339	5	13	theme	bacterial	736:744	arg1	microbiomes					757:767	the bacterial and fungal microbiomes	732:767	the bacterial and fungal microbiomes	732:767	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	2	14	theme	bacterium	301:309	arg1	mesenteroides					323:335	the bacterium Leuconostoc mesenteroides	297:335	the bacterium Leuconostoc mesenteroides as the main contributor to both processes	297:377	Historically, culture-based approaches have focused on the bacterium Leuconostoc mesenteroides as the main contributor to both processes.					
37162339	1	15	theme	undesirable	194:204	arg1	production					230:239	undesirable exopolysaccharide (EPS) production	194:239	undesirable exopolysaccharide (EPS) production	194:239	During postharvest processing of sugarcane for raw sugar, microbial activity results in sucrose loss and undesirable exopolysaccharide (EPS) production.					
37162339	8	16	theme	long-ignored	1471:1482	arg1	gaps					1484:1487	long-ignored gaps	1471:1487	long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories	1471:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	7	17	theme	microbial	1387:1395	arg1	composition					1397:1407	microbial composition	1387:1407	microbial composition	1387:1407	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	5	18	theme	fungal	750:755	arg1	microbiomes					757:767	the bacterial and fungal microbiomes	732:767	the bacterial and fungal microbiomes	732:767	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	5	19	theme	sequence	847:854	arg1	ASVs					866:869	ASVs	866:869	ASVs	866:869	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	5	19	theme	sequence	847:854	arg1	variants					856:863	417 bacterial amplicon sequence variants	824:863	417 bacterial amplicon sequence variants (ASVs)	824:870	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	9	20	theme	raw	1795:1797	arg1	factories					1805:1813	raw sugar factories	1795:1813	raw sugar factories	1795:1813	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	4	21	theme	amplicon-based	568:581	arg1	profiling					592:600	high-throughput amplicon-based sequence profiling	552:600	high-throughput amplicon-based sequence profiling	552:600	In the present study, high-throughput amplicon-based sequence profiling was applied to gain a more comprehensive view of the microbial community in Louisiana raw sugar factories.					
37162339	5	22	theme	Microbial	709:717	arg1	profiling					719:727	Microbial profiling	709:727	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences	709:796	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	1	23	theme	sugarcane	122:130	arg1	processing					108:117	postharvest processing	96:117	postharvest processing of sugarcane for raw sugar	96:144	During postharvest processing of sugarcane for raw sugar, microbial activity results in sucrose loss and undesirable exopolysaccharide (EPS) production.					
37162339	7	24	from	rate	1235:1238	arg1	differences					1372:1382	the observed differences	1359:1382	the observed differences in microbial composition	1359:1407	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	2	25	theme	culture-based	256:268	arg1	approaches					270:279	culture-based approaches	256:279	culture-based approaches	256:279	Historically, culture-based approaches have focused on the bacterium Leuconostoc mesenteroides as the main contributor to both processes.					
37162339	1	26	theme	raw	136:138	arg1	sugar					140:144	raw sugar	136:144	raw sugar	136:144	During postharvest processing of sugarcane for raw sugar, microbial activity results in sucrose loss and undesirable exopolysaccharide (EPS) production.					
37162339	7	27	theme	result	1347:1352	arg1	rate					1235:1238	the rate	1231:1238	the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition	1231:1407	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	5	28	theme	ITS1	783:786	arg1	sequences					788:796	16S V4 and ITS1 sequences	772:796	16S V4 and ITS1 sequences	772:796	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	0	29	theme	Sugar	72:76	arg1	Factories					78:86	Louisiana Raw Sugar Factories	58:86	Louisiana Raw Sugar Factories	58:86	Microbiome Analysis of Sugarcane Juices and Biofilms from Louisiana Raw Sugar Factories.					
37162339	6	30	theme	multiple	990:997	arg1	samples					999:1005	multiple samples	990:1005	multiple samples	990:1005	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	8	31	theme	present	1550:1556	arg1	microbes					1541:1548	microbes	1541:1548	microbes present in processing streams at Louisiana sugarcane raw sugar factories	1541:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	9	32	theme	biocontrol	1849:1858	arg1	measures					1860:1867	biocontrol measures	1849:1867	biocontrol measures	1849:1867	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	8	33	theme	processing	1561:1570	arg1	streams					1572:1578	processing streams	1561:1578	processing streams at Louisiana sugarcane raw sugar factories	1561:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	5	34	theme	V4	776:777	arg1	sequences					788:796	16S V4 and ITS1 sequences	772:796	16S V4 and ITS1 sequences	772:796	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	7	35	from	differences	1216:1226	arg1	rate					1235:1238	the rate	1231:1238	the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition	1231:1407	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	5	36	theme	amplicon	838:845	arg1	ASVs					866:869	ASVs	866:869	ASVs	866:869	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	5	36	theme	amplicon	838:845	arg1	variants					856:863	417 bacterial amplicon sequence variants	824:863	417 bacterial amplicon sequence variants (ASVs)	824:870	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	3	37	located	present	441:447	arg2	microbes					428:435	diverse microbes	420:435	diverse microbes	420:435	However, recent studies have shown that diverse microbes are present in sugarcane factories and may also contribute to sugarcane juice deterioration.					
37162339	3	37	located	present	441:447	arg1	factories					462:470	sugarcane factories	452:470	sugarcane factories	452:470	However, recent studies have shown that diverse microbes are present in sugarcane factories and may also contribute to sugarcane juice deterioration.					
37162339	7	38	from	differences	1372:1382	arg1	rate					1235:1238	the rate	1231:1238	the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition	1231:1407	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	7	38	from	differences	1372:1382	arg1	composition					1397:1407	microbial composition	1387:1407	microbial composition	1387:1407	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	9	39	theme	sucrose	1881:1887	arg1	losses					1889:1894	sucrose losses	1881:1894	sucrose losses	1881:1894	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	8	40	theme	sugarcane	1593:1601	arg1	factories					1613:1621	Louisiana sugarcane raw sugar factories	1583:1621	Louisiana sugarcane raw sugar factories	1583:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	7	41	theme	organic	1322:1328	arg1	acids					1330:1334	other organic acids	1316:1334	other organic acids	1316:1334	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	3	42	theme	diverse	420:426	arg1	microbes					428:435	diverse microbes	420:435	diverse microbes	420:435	However, recent studies have shown that diverse microbes are present in sugarcane factories and may also contribute to sugarcane juice deterioration.					
37162339	8	43	theme	sugar	1607:1611	arg1	factories					1613:1621	Louisiana sugarcane raw sugar factories	1583:1621	Louisiana sugarcane raw sugar factories	1583:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	6	44	attach	present	1110:1116	arg1	factories					1131:1139	sugarcane factories	1121:1139	sugarcane factories	1121:1139	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	6	44	attach	present	1110:1116	arg2	diversity					1100:1108	the microbial diversity	1086:1108	the microbial diversity present in sugarcane factories	1086:1139	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	9	45	from	studies	1838:1844	arg1	measures					1860:1867	biocontrol measures	1849:1867	biocontrol measures	1849:1867	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	9	46	theme	emerging	1649:1656	arg1	model					1658:1662	an emerging model	1646:1662	an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production	1646:1957	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	4	47	theme	present	537:543	arg1	study					545:549	the present study	533:549	the present study	533:549	In the present study, high-throughput amplicon-based sequence profiling was applied to gain a more comprehensive view of the microbial community in Louisiana raw sugar factories.					
37162339	7	48	theme	sucrose	1243:1249	arg1	consumption					1251:1261	sucrose consumption	1243:1261	sucrose consumption	1243:1261	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	8	49	from	present	1550:1556	arg1	streams					1572:1578	processing streams	1561:1578	processing streams at Louisiana sugarcane raw sugar factories	1561:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	3	50	theme	sugarcane	499:507	arg1	deterioration					515:527	sugarcane juice deterioration	499:527	sugarcane juice deterioration	499:527	However, recent studies have shown that diverse microbes are present in sugarcane factories and may also contribute to sugarcane juice deterioration.					
37162339	6	51	from	factories	1131:1139	arg1	present					1110:1116	present	1110:1116	present	1110:1116	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	2	52	theme	main	344:347	arg1	contributor					349:359	the main contributor	340:359	the main contributor to both processes	340:377	Historically, culture-based approaches have focused on the bacterium Leuconostoc mesenteroides as the main contributor to both processes.					
37162339	3	53	from	present	441:447	arg1	factories					462:470	sugarcane factories	452:470	sugarcane factories	452:470	However, recent studies have shown that diverse microbes are present in sugarcane factories and may also contribute to sugarcane juice deterioration.					
37162339	6	54	theme	bacterial	940:948	arg1	genus					950:954	the most abundant bacterial genus	922:954	the most abundant bacterial genus	922:954	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	6	54	theme	bacterial	940:948	arg1	Leuconostoc					899:909	Leuconostoc	899:909	Leuconostoc	899:909	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	8	55	from	factories	1613:1621	arg1	streams					1572:1578	processing streams	1561:1578	processing streams at Louisiana sugarcane raw sugar factories	1561:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	9	56	theme	juice	1712:1716	arg1	degradation					1718:1728	sugarcane juice degradation	1702:1728	sugarcane juice degradation	1702:1728	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	8	57	dep	IMPORTANCE	1410:1419	arg1	sequencing					1436:1445	Amplicon-based sequencing	1421:1445	IMPORTANCE Amplicon-based sequencing	1410:1445	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	4	58	theme	Louisiana	678:686	arg1	factories					698:706	Louisiana raw sugar factories	678:706	Louisiana raw sugar factories	678:706	In the present study, high-throughput amplicon-based sequence profiling was applied to gain a more comprehensive view of the microbial community in Louisiana raw sugar factories.					
37162339	1	59	theme	sucrose	177:183	arg1	loss					185:188	sucrose loss	177:188	sucrose loss	177:188	During postharvest processing of sugarcane for raw sugar, microbial activity results in sucrose loss and undesirable exopolysaccharide (EPS) production.					
37162339	6	60	theme	sequences	978:986	arg1	%					969:969	40.9%	965:969	40.9% of 16S sequences	965:986	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	6	60	theme	sequences	978:986	arg1	sequences					978:986	16S sequences	974:986	16S sequences	974:986	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	9	61	theme	microbial	1753:1761	arg1	problems					1777:1784	microbial contamination problems	1753:1784	microbial contamination problems faced by raw sugar factories	1753:1813	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	3	62	attach	present	441:447	arg2	microbes					428:435	diverse microbes	420:435	diverse microbes	420:435	However, recent studies have shown that diverse microbes are present in sugarcane factories and may also contribute to sugarcane juice deterioration.					
37162339	3	62	attach	present	441:447	arg1	factories					462:470	sugarcane factories	452:470	sugarcane factories	452:470	However, recent studies have shown that diverse microbes are present in sugarcane factories and may also contribute to sugarcane juice deterioration.					
37162339	4	63	theme	sugar	692:696	arg1	factories					698:706	Louisiana raw sugar factories	678:706	Louisiana raw sugar factories	678:706	In the present study, high-throughput amplicon-based sequence profiling was applied to gain a more comprehensive view of the microbial community in Louisiana raw sugar factories.					
37162339	2	64	theme	Leuconostoc	311:321	arg1	mesenteroides					323:335	the bacterium Leuconostoc mesenteroides	297:335	the bacterium Leuconostoc mesenteroides as the main contributor to both processes	297:377	Historically, culture-based approaches have focused on the bacterium Leuconostoc mesenteroides as the main contributor to both processes.					
37162339	6	65	dep	overall	956:962	arg1	%					969:969	40.9%	965:969	40.9% of 16S sequences	965:986	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	6	65	dep	overall	956:962	arg1	sequences					978:986	16S sequences	974:986	16S sequences	974:986	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	1	66	theme	exopolysaccharide	206:222	arg1	production					230:239	undesirable exopolysaccharide (EPS) production	194:239	undesirable exopolysaccharide (EPS) production	194:239	During postharvest processing of sugarcane for raw sugar, microbial activity results in sucrose loss and undesirable exopolysaccharide (EPS) production.					
37162339	8	67	theme	microbiological	1492:1506	arg1	knowledge					1508:1516	microbiological knowledge	1492:1516	microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories	1492:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	4	68	theme	community	665:673	arg1	view					643:646	a more comprehensive view	622:646	a more comprehensive view of the microbial community in Louisiana raw sugar factories	622:706	In the present study, high-throughput amplicon-based sequence profiling was applied to gain a more comprehensive view of the microbial community in Louisiana raw sugar factories.					
37162339	9	69	theme	sugar	1799:1803	arg1	factories					1805:1813	raw sugar factories	1795:1813	raw sugar factories	1795:1813	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	6	70	theme	other	1025:1029	arg1	Lactobacillus					1058:1070	Lactobacillus	1058:1070	Lactobacillus	1058:1070	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	6	70	theme	other	1025:1029	arg1	taxa					1031:1034	other taxa	1025:1034	other taxa such as Weissella and Lactobacillus	1025:1070	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	6	70	theme	other	1025:1029	arg1	Weissella					1044:1052	Weissella	1044:1052	Weissella	1044:1052	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	7	71	theme	observed	1363:1370	arg1	differences					1372:1382	the observed differences	1359:1382	the observed differences in microbial composition	1359:1407	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	0	72	theme	Raw	68:70	arg1	Factories					78:86	Louisiana Raw Sugar Factories	58:86	Louisiana Raw Sugar Factories	58:86	Microbiome Analysis of Sugarcane Juices and Biofilms from Louisiana Raw Sugar Factories.					
37162339	8	73	theme	microbes	1541:1548	arg1	diversity					1528:1536	the diversity	1524:1536	the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories	1524:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	8	74	from	gaps	1484:1487	arg1	knowledge					1508:1516	microbiological knowledge	1492:1516	microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories	1492:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	1	75	theme	microbial	147:155	arg1	activity					157:164	microbial activity	147:164	microbial activity	147:164	During postharvest processing of sugarcane for raw sugar, microbial activity results in sucrose loss and undesirable exopolysaccharide (EPS) production.					
37162339	9	76	theme	future	1831:1836	arg1	studies					1838:1844	future studies	1831:1844	future studies on biocontrol measures	1831:1867	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	0	77	from	Factories	78:86	arg1	Biofilms					44:51	and Biofilms	40:51	Biofilms	44:51	Microbiome Analysis of Sugarcane Juices and Biofilms from Louisiana Raw Sugar Factories.					
37162339	5	78	theme	microbiomes	757:767	arg1	profiling					719:727	Microbial profiling	709:727	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences	709:796	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	5	79	theme	16S	772:774	arg1	sequences					788:796	16S V4 and ITS1 sequences	772:796	16S V4 and ITS1 sequences	772:796	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	8	80	theme	Louisiana	1583:1591	arg1	factories					1613:1621	Louisiana sugarcane raw sugar factories	1583:1621	Louisiana sugarcane raw sugar factories	1583:1621	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	3	81	theme	sugarcane	452:460	arg1	factories					462:470	sugarcane factories	452:470	sugarcane factories	452:470	However, recent studies have shown that diverse microbes are present in sugarcane factories and may also contribute to sugarcane juice deterioration.					
37162339	5	82	theme	bacterial	828:836	arg1	ASVs					866:869	ASVs	866:869	ASVs	866:869	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	5	82	theme	bacterial	828:836	arg1	variants					856:863	417 bacterial amplicon sequence variants	824:863	417 bacterial amplicon sequence variants (ASVs)	824:870	Microbial profiling of the bacterial and fungal microbiomes by 16S V4 and ITS1 sequences, respectively, identified 417 bacterial amplicon sequence variants (ASVs) and 793 fungal ASVs.					
37162339	7	83	theme	acids	1330:1334	arg1	consumption					1251:1261	sucrose consumption	1243:1261	sucrose consumption	1243:1261	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	7	83	theme	acids	1330:1334	arg1	production					1279:1288	the production	1275:1288	the production of exopolysaccharides and other organic acids	1275:1334	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	4	84	theme	sequence	583:590	arg1	profiling					592:600	high-throughput amplicon-based sequence profiling	552:600	high-throughput amplicon-based sequence profiling	552:600	In the present study, high-throughput amplicon-based sequence profiling was applied to gain a more comprehensive view of the microbial community in Louisiana raw sugar factories.					
37162339	0	85	theme	Sugarcane	23:31	arg1	Analysis					11:18	Microbiome Analysis	0:18	Microbiome Analysis of Sugarcane	0:31	Microbiome Analysis of Sugarcane Juices and Biofilms from Louisiana Raw Sugar Factories.					
37162339	1	86	theme	postharvest	96:106	arg1	processing					108:117	postharvest processing	96:117	postharvest processing of sugarcane for raw sugar	96:144	During postharvest processing of sugarcane for raw sugar, microbial activity results in sucrose loss and undesirable exopolysaccharide (EPS) production.					
37162339	7	87	theme	other	1316:1320	arg1	acids					1330:1334	other organic acids	1316:1334	other organic acids	1316:1334	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	3	88	theme	recent	389:394	arg1	studies					396:402	recent studies	389:402	recent studies	389:402	However, recent studies have shown that diverse microbes are present in sugarcane factories and may also contribute to sugarcane juice deterioration.					
37162339	7	89	theme	flask	1155:1159	arg1	cultures					1161:1168	flask cultures	1155:1168	flask cultures inoculated with the same samples	1155:1201	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	7	90	theme	exopolysaccharides	1293:1310	arg1	consumption					1251:1261	sucrose consumption	1243:1261	sucrose consumption	1243:1261	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	7	90	theme	exopolysaccharides	1293:1310	arg1	production					1279:1288	the production	1275:1288	the production of exopolysaccharides and other organic acids	1275:1334	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	9	91	theme	operational	1900:1910	arg1	challenges					1912:1921	operational challenges	1900:1921	operational challenges	1900:1921	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	3	92	from	factories	462:470	arg1	present					441:447	present	441:447	present	441:447	However, recent studies have shown that diverse microbes are present in sugarcane factories and may also contribute to sugarcane juice deterioration.					
37162339	8	93	from	streams	1572:1578	arg1	present					1550:1556	present	1550:1556	present	1550:1556	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	0	94	theme	Louisiana	58:66	arg1	Factories					78:86	Louisiana Raw Sugar Factories	58:86	Louisiana Raw Sugar Factories	58:86	Microbiome Analysis of Sugarcane Juices and Biofilms from Louisiana Raw Sugar Factories.					
37162339	7	95	theme	production	1279:1288	arg1	rate					1235:1238	the rate	1231:1238	the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition	1231:1407	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
37162339	9	96	theme	due	1923:1925	arg1	losses					1889:1894	sucrose losses	1881:1894	sucrose losses	1881:1894	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	4	97	theme	high-throughput	552:566	arg1	profiling					592:600	high-throughput amplicon-based sequence profiling	552:600	high-throughput amplicon-based sequence profiling	552:600	In the present study, high-throughput amplicon-based sequence profiling was applied to gain a more comprehensive view of the microbial community in Louisiana raw sugar factories.					
37162339	8	98	theme	Amplicon-based	1421:1434	arg1	sequencing					1436:1445	Amplicon-based sequencing	1421:1445	IMPORTANCE Amplicon-based sequencing	1410:1445	IMPORTANCE Amplicon-based sequencing was utilized to address long-ignored gaps in microbiological knowledge about the diversity of microbes present in processing streams at Louisiana sugarcane raw sugar factories.					
37162339	3	99	theme	juice	509:513	arg1	deterioration					515:527	sugarcane juice deterioration	499:527	sugarcane juice deterioration	499:527	However, recent studies have shown that diverse microbes are present in sugarcane factories and may also contribute to sugarcane juice deterioration.					
37162339	9	100	theme	diverse	1670:1676	arg1	organisms					1678:1686	diverse organisms	1670:1686	diverse organisms	1670:1686	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	4	101	from	view	643:646	arg1	factories					698:706	Louisiana raw sugar factories	678:706	Louisiana raw sugar factories	678:706	In the present study, high-throughput amplicon-based sequence profiling was applied to gain a more comprehensive view of the microbial community in Louisiana raw sugar factories.					
37162339	6	102	theme	sugarcane	1121:1129	arg1	factories					1131:1139	sugarcane factories	1121:1139	sugarcane factories	1121:1139	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	9	103	theme	exopolysaccharide	1930:1946	arg1	production					1948:1957	exopolysaccharide production	1930:1957	exopolysaccharide production	1930:1957	These results support an emerging model where diverse organisms contribute to sugarcane juice degradation, help to contextualize microbial contamination problems faced by raw sugar factories, and will guide future studies on biocontrol measures to mitigate sucrose losses and operational challenges due to exopolysaccharide production.					
37162339	4	104	theme	comprehensive	629:641	arg1	view					643:646	a more comprehensive view	622:646	a more comprehensive view of the microbial community in Louisiana raw sugar factories	622:706	In the present study, high-throughput amplicon-based sequence profiling was applied to gain a more comprehensive view of the microbial community in Louisiana raw sugar factories.					
37162339	6	105	theme	present	1110:1116	arg1	diversity					1100:1108	the microbial diversity	1086:1108	the microbial diversity present in sugarcane factories	1086:1139	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	6	106	from	present	1110:1116	arg1	factories					1131:1139	sugarcane factories	1121:1139	sugarcane factories	1121:1139	While Leuconostoc was indeed the most abundant bacterial genus overall (40.9% of 16S sequences), multiple samples were dominated by other taxa such as Weissella and Lactobacillus, underscoring the microbial diversity present in sugarcane factories.					
37162339	7	107	theme	same	1190:1193	arg1	samples					1195:1201	the same samples	1186:1201	the same samples	1186:1201	Furthermore, flask cultures inoculated with the same samples demonstrated differences in the rate of sucrose consumption, as well as the production of exopolysaccharides and other organic acids, which may result from the observed differences in microbial composition.					
34998558	10	0	from	cheeses	1756:1762	arg1	predominant					1737:1747	predominant	1737:1747	predominant	1737:1747	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	7	1	theme	composition	1181:1191	arg1	analysis					1146:1153	quantitative analysis	1133:1153	quantitative analysis of chemical and microbial composition	1133:1191	Analysis for each sample were performed for quantitative analysis of chemical and microbial composition.					
34998558	10	2	theme	aging	1958:1962	arg1	d					1953:1953	90 d	1950:1953	90 d of aging	1950:1962	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	9	3	theme	similar	1532:1538	arg1	properties					1549:1558	similar chemical properties	1532:1558	similar chemical properties	1532:1558	Fructooligosaccharides supplemented cheeses showed similar chemical properties to the control cheese, whereas inulin-supplemented cheeses exhibited a significantly higher moisture content than FOS and the control groups.					
34998558	10	4	theme	acid	1861:1864	arg1	bacteria					1866:1873	nonstarter lactic acid bacteria	1843:1873	nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging	1843:1962	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	8	5	dep	±	1291:1291	arg1	deviation					1326:1334	± standard deviation	1315:1334	± standard deviation	1315:1334	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	10	6	contain	possessed	1810:1818	arg2	amounts					1832:1838	significant amounts	1820:1838	significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging	1820:1962	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	10	6	contain	possessed	1810:1818	arg2	bacteria					1866:1873	nonstarter lactic acid bacteria	1843:1873	nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging	1843:1962	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	10	6	contain	possessed	1810:1818	arg1	cheeses					1802:1808	2% inulin and 2% FOS-supplemented cheeses	1768:1808	2% inulin and 2% FOS-supplemented cheeses	1768:1808	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	8	7	theme	lactic	1212:1217	arg1	cfu/g					1238:1242	log cfu/g	1234:1242	log cfu/g	1234:1242	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	8	7	theme	lactic	1212:1217	arg1	bacteria					1224:1231	lactic acid bacteria	1212:1231	lactic acid bacteria (log cfu/g)	1212:1243	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	10	8	theme	nonstarter	1843:1852	arg1	bacteria					1866:1873	nonstarter lactic acid bacteria	1843:1873	nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging	1843:1962	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	2	9	theme	water	283:287	arg1	matrix					289:294	this fat, protein, and water matrix	260:294	this fat, protein, and water matrix	260:294	Microorganisms within this fat, protein, and water matrix are largely responsible for physiochemical characteristics and associated quality.					
34998558	8	10	theme	bacteria	1224:1231	arg1	prevalence					1198:1207	The prevalence	1194:1207	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00)	1194:1375	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	8	10	theme	bacteria	1224:1231	arg1	higher					1395:1400	higher	1395:1400	higher	1395:1400	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	8	11	from	prevalence	1198:1207	arg1	cheese					1256:1261	Cheddar cheese	1248:1261	Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00)	1248:1375	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	8	12	theme	log	1234:1236	arg1	cfu/g					1238:1242	log cfu/g	1234:1242	log cfu/g	1234:1242	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	8	12	theme	log	1234:1236	arg1	bacteria					1224:1231	lactic acid bacteria	1212:1231	lactic acid bacteria (log cfu/g)	1212:1243	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	0	13	from	milk	99:102	arg1	prebiotics					71:80	prebiotics	71:80	prebiotics from pasteurized milk to aging	71:111	Microbial and chemical composition of Cheddar cheese supplemented with prebiotics from pasteurized milk to aging.					
34998558	8	14	theme	Cheddar	1248:1254	arg1	cheese					1256:1261	Cheddar cheese	1248:1261	Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00)	1248:1375	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	1	15	from	industry	182:189	arg1	crucial					161:167	crucial	161:167	crucial	161:167	Microbial and chemical properties of cheese is crucial in the dairy industry to understand their effects on cheese quality.					
34998558	10	16	theme	unidentified	1890:1901	arg1	group					1903:1907	an unidentified group	1887:1907	an unidentified group of Lactobacillaceae, which emerged after 90 d of aging	1887:1962	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	10	16	theme	unidentified	1890:1901	arg1	Lactobacillaceae					1912:1927	Lactobacillaceae	1912:1927	Lactobacillaceae	1912:1927	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	7	17	theme	microbial	1171:1179	arg1	composition					1181:1191	chemical and microbial composition	1158:1191	chemical and microbial composition	1158:1191	Analysis for each sample were performed for quantitative analysis of chemical and microbial composition.					
34998558	1	18	theme	cheese	151:156	arg1	properties					137:146	Microbial and chemical properties	114:146	Microbial and chemical properties of cheese	114:156	Microbial and chemical properties of cheese is crucial in the dairy industry to understand their effects on cheese quality.					
34998558	9	19	theme	higher	1645:1650	arg1	content					1661:1667	a significantly higher moisture content	1629:1667	a significantly higher moisture content	1629:1667	Fructooligosaccharides supplemented cheeses showed similar chemical properties to the control cheese, whereas inulin-supplemented cheeses exhibited a significantly higher moisture content than FOS and the control groups.					
34998558	5	20	theme	Laboratory-scale	778:793	arg1	cheese					803:808	Laboratory-scale Cheddar cheese	778:808	Laboratory-scale Cheddar cheese produced in 2	778:822	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	3	21	theme	acid	433:436	arg1	bacteria					438:445	lactic acid bacteria	426:445	lactic acid bacteria in cheese	426:455	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	0	22	theme	Cheddar	38:44	arg1	cheese					46:51	Cheddar cheese	38:51	Cheddar cheese	38:51	Microbial and chemical composition of Cheddar cheese supplemented with prebiotics from pasteurized milk to aging.					
34998558	4	23	theme	microbial	727:735	arg1	changes					769:775	microbial and physicochemical composition changes	727:775	microbial and physicochemical composition changes	727:775	This research focuses on the addition of fructooligosaccharides (FOS) or inulin to the Cheddar cheese-making process to evaluate the effects on microbial and physicochemical composition changes.					
34998558	0	24	theme	Microbial	0:8	arg1	composition					23:33	Microbial and chemical composition	0:33	composition	23:33	Microbial and chemical composition of Cheddar cheese supplemented with prebiotics from pasteurized milk to aging.					
34998558	5	25	theme	FOS	900:902	arg1	inulin					907:912	inulin	907:912	inulin	907:912	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	5	25	theme	FOS	900:902	arg1	wt/wt					890:894	wt/wt	890:894	wt/wt	890:894	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	5	25	theme	FOS	900:902	arg1	FOS					900:902	FOS	900:902	FOS	900:902	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	5	25	theme	FOS	900:902	arg1	%					887:887	0.5, 1.0, and 2.0%	870:887	0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin	870:912	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	5	25	theme	FOS	900:902	arg1	0					857:857	0	857:857	0	857:857	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	11	26	theme	Cheddar	2038:2044	arg1	cheese					2046:2051	Cheddar cheese	2038:2051	Cheddar cheese	2038:2051	In conclusion, this study demonstrates that prebiotic supplementation of Cheddar cheese results in differing microbial and chemical characteristics.					
34998558	4	27	theme	physicochemical	741:755	arg1	changes					769:775	microbial and physicochemical composition changes	727:775	microbial and physicochemical composition changes	727:775	This research focuses on the addition of fructooligosaccharides (FOS) or inulin to the Cheddar cheese-making process to evaluate the effects on microbial and physicochemical composition changes.					
34998558	0	28	theme	chemical	14:21	arg1	composition					23:33	Microbial and chemical composition	0:33	composition	23:33	Microbial and chemical composition of Cheddar cheese supplemented with prebiotics from pasteurized milk to aging.					
34998558	3	29	theme	value-added	523:533	arg1	foods					535:539	value-added foods	523:539	value-added foods	523:539	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	5	30	theme	inulin	907:912	arg1	inulin					907:912	inulin	907:912	inulin	907:912	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	5	30	theme	inulin	907:912	arg1	wt/wt					890:894	wt/wt	890:894	wt/wt	890:894	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	5	30	theme	inulin	907:912	arg1	FOS					900:902	FOS	900:902	FOS	900:902	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	5	30	theme	inulin	907:912	arg1	%					887:887	0.5, 1.0, and 2.0%	870:887	0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin	870:912	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	5	30	theme	inulin	907:912	arg1	0					857:857	0	857:857	0	857:857	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	4	31	theme	Cheddar	670:676	arg1	process					692:698	the Cheddar cheese-making process	666:698	the Cheddar cheese-making process	666:698	This research focuses on the addition of fructooligosaccharides (FOS) or inulin to the Cheddar cheese-making process to evaluate the effects on microbial and physicochemical composition changes.					
34998558	3	32	theme	energy	408:413	arg1	Prebiotics					379:388	Prebiotics	379:388	Prebiotics	379:388	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	3	32	theme	energy	408:413	arg1	potential					509:517	the potential	505:517	the potential for value-added foods, with a more stable probiotic population	505:580	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	3	32	theme	energy	408:413	arg1	source					415:420	an energy source	405:420	an energy source for lactic acid bacteria in cheese	405:455	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	3	33	with	foods	535:539	arg1	population					571:580	a more stable probiotic population	547:580	a more stable probiotic population	547:580	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	6	34	theme	samples	974:980	arg1	total					961:965	A total	959:965	A total of 210 samples (15 samples per replicate of each treatment)	959:1025	A total of 210 samples (15 samples per replicate of each treatment) were collected from cheese-making procedure and aging period.					
34998558	8	35	theme	±	1315:1315	arg1	deviation					1326:1334	± standard deviation	1315:1334	± standard deviation	1315:1334	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	0	36	theme	cheese	46:51	arg1	composition					23:33	Microbial and chemical composition	0:33	composition	23:33	Microbial and chemical composition of Cheddar cheese supplemented with prebiotics from pasteurized milk to aging.					
34998558	10	37	theme	%	1769:1769	arg1	cheeses					1802:1808	2% inulin and 2% FOS-supplemented cheeses	1768:1808	2% inulin and 2% FOS-supplemented cheeses	1768:1808	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	8	38	dep	control	1411:1417	arg1	±					1425:1425	5.84 ± 0.27	1420:1430	5.84 ± 0.27	1420:1430	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	8	38	dep	control	1411:1417	arg1	±					1441:1441	8.48 ± 0.06	1436:1446	8.48 ± 0.06	1436:1446	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	5	39	theme	pasteurized	941:951	arg1	milk					953:956	commercially pasteurized milk	928:956	commercially pasteurized milk	928:956	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	10	40	from	predominant	1737:1747	arg1	cheeses					1756:1762	all cheeses	1752:1762	all cheeses	1752:1762	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	10	41	theme	%	1783:1783	arg1	cheeses					1802:1808	2% inulin and 2% FOS-supplemented cheeses	1768:1808	2% inulin and 2% FOS-supplemented cheeses	1768:1808	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	3	42	from	bacteria	438:445	arg1	cheese					450:455	cheese	450:455	cheese	450:455	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	2	43	theme	associated	359:368	arg1	quality					370:376	associated quality	359:376	associated quality	359:376	Microorganisms within this fat, protein, and water matrix are largely responsible for physiochemical characteristics and associated quality.					
34998558	10	44	theme	significant	1820:1830	arg1	bacteria					1866:1873	nonstarter lactic acid bacteria	1843:1873	nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging	1843:1962	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	10	44	theme	significant	1820:1830	arg1	amounts					1832:1838	significant amounts	1820:1838	significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging	1820:1962	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	2	45	theme	protein	270:276	arg1	matrix					289:294	this fat, protein, and water matrix	260:294	this fat, protein, and water matrix	260:294	Microorganisms within this fat, protein, and water matrix are largely responsible for physiochemical characteristics and associated quality.					
34998558	9	46	theme	chemical	1540:1547	arg1	properties					1549:1558	similar chemical properties	1532:1558	similar chemical properties	1532:1558	Fructooligosaccharides supplemented cheeses showed similar chemical properties to the control cheese, whereas inulin-supplemented cheeses exhibited a significantly higher moisture content than FOS and the control groups.					
34998558	7	47	theme	chemical	1158:1165	arg1	composition					1181:1191	chemical and microbial composition	1158:1191	chemical and microbial composition	1158:1191	Analysis for each sample were performed for quantitative analysis of chemical and microbial composition.					
34998558	3	48	theme	stable	554:559	arg1	population					571:580	a more stable probiotic population	547:580	a more stable probiotic population	547:580	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	1	49	theme	dairy	176:180	arg1	industry					182:189	the dairy industry	172:189	the dairy industry	172:189	Microbial and chemical properties of cheese is crucial in the dairy industry to understand their effects on cheese quality.					
34998558	11	50	theme	prebiotic	2009:2017	arg1	supplementation					2019:2033	prebiotic supplementation	2009:2033	prebiotic supplementation of Cheddar cheese	2009:2051	In conclusion, this study demonstrates that prebiotic supplementation of Cheddar cheese results in differing microbial and chemical characteristics.					
34998558	4	51	theme	inulin	656:661	arg1	addition					612:619	the addition	608:619	the addition of fructooligosaccharides (FOS) or inulin to the Cheddar cheese-making process	608:698	This research focuses on the addition of fructooligosaccharides (FOS) or inulin to the Cheddar cheese-making process to evaluate the effects on microbial and physicochemical composition changes.					
34998558	10	52	theme	lactic	1854:1859	arg1	bacteria					1866:1873	nonstarter lactic acid bacteria	1843:1873	nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging	1843:1962	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	9	53	theme	control	1567:1573	arg1	cheese					1575:1580	the control cheese	1563:1580	the control cheese	1563:1580	Fructooligosaccharides supplemented cheeses showed similar chemical properties to the control cheese, whereas inulin-supplemented cheeses exhibited a significantly higher moisture content than FOS and the control groups.					
34998558	6	54	theme	aging	1075:1079	arg1	period					1081:1086	aging period	1075:1086	aging period	1075:1086	A total of 210 samples (15 samples per replicate of each treatment) were collected from cheese-making procedure and aging period.					
34998558	8	55	theme	acid	1219:1222	arg1	cfu/g					1238:1242	log cfu/g	1234:1242	log cfu/g	1234:1242	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	8	55	theme	acid	1219:1222	arg1	bacteria					1224:1231	lactic acid bacteria	1212:1231	lactic acid bacteria (log cfu/g)	1212:1243	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	1	56	from	effects	211:217	arg1	quality					229:235	cheese quality	222:235	cheese quality	222:235	Microbial and chemical properties of cheese is crucial in the dairy industry to understand their effects on cheese quality.					
34998558	1	57	theme	Microbial	114:122	arg1	properties					137:146	Microbial and chemical properties	114:146	Microbial and chemical properties of cheese	114:156	Microbial and chemical properties of cheese is crucial in the dairy industry to understand their effects on cheese quality.					
34998558	9	58	theme	inulin-supplemented	1591:1609	arg1	cheeses					1611:1617	inulin-supplemented cheeses	1591:1617	inulin-supplemented cheeses	1591:1617	Fructooligosaccharides supplemented cheeses showed similar chemical properties to the control cheese, whereas inulin-supplemented cheeses exhibited a significantly higher moisture content than FOS and the control groups.					
34998558	10	59	theme	bacteria	1866:1873	arg1	bacteria					1866:1873	nonstarter lactic acid bacteria	1843:1873	nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging	1843:1962	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	10	59	theme	bacteria	1866:1873	arg1	amounts					1832:1838	significant amounts	1820:1838	significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging	1820:1962	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	2	60	theme	fat	265:267	arg1	matrix					289:294	this fat, protein, and water matrix	260:294	this fat, protein, and water matrix	260:294	Microorganisms within this fat, protein, and water matrix are largely responsible for physiochemical characteristics and associated quality.					
34998558	1	61	theme	chemical	128:135	arg1	properties					137:146	Microbial and chemical properties	114:146	Microbial and chemical properties of cheese	114:156	Microbial and chemical properties of cheese is crucial in the dairy industry to understand their effects on cheese quality.					
34998558	4	62	theme	fructooligosaccharides	624:645	arg1	addition					612:619	the addition	608:619	the addition of fructooligosaccharides (FOS) or inulin to the Cheddar cheese-making process	608:698	This research focuses on the addition of fructooligosaccharides (FOS) or inulin to the Cheddar cheese-making process to evaluate the effects on microbial and physicochemical composition changes.					
34998558	0	63	theme	pasteurized	87:97	arg1	milk					99:102	pasteurized milk	87:102	pasteurized milk to aging	87:111	Microbial and chemical composition of Cheddar cheese supplemented with prebiotics from pasteurized milk to aging.					
34998558	3	64	dep	stable	554:559	arg1	probiotic					561:569	probiotic	561:569	probiotic	561:569	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	9	65	theme	moisture	1652:1659	arg1	content					1661:1667	a significantly higher moisture content	1629:1667	a significantly higher moisture content	1629:1667	Fructooligosaccharides supplemented cheeses showed similar chemical properties to the control cheese, whereas inulin-supplemented cheeses exhibited a significantly higher moisture content than FOS and the control groups.					
34998558	4	66	theme	cheese-making	678:690	arg1	process					692:698	the Cheddar cheese-making process	666:698	the Cheddar cheese-making process	666:698	This research focuses on the addition of fructooligosaccharides (FOS) or inulin to the Cheddar cheese-making process to evaluate the effects on microbial and physicochemical composition changes.					
34998558	10	67	attach	predominant	1737:1747	arg2	Lactococcus					1720:1730	Lactococcus	1720:1730	Lactococcus	1720:1730	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	10	67	attach	predominant	1737:1747	arg1	cheeses					1756:1762	all cheeses	1752:1762	all cheeses	1752:1762	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	10	67	attach	predominant	1737:1747	arg2	Streptococcus					1702:1714	Streptococcus	1702:1714	Streptococcus	1702:1714	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	7	68	theme	quantitative	1133:1144	arg1	analysis					1146:1153	quantitative analysis	1133:1153	quantitative analysis of chemical and microbial composition	1133:1191	Analysis for each sample were performed for quantitative analysis of chemical and microbial composition.					
34998558	1	69	from	crucial	161:167	arg1	industry					182:189	the dairy industry	172:189	the dairy industry	172:189	Microbial and chemical properties of cheese is crucial in the dairy industry to understand their effects on cheese quality.					
34998558	6	70	dep	samples	974:980	arg1	samples					986:992	15 samples	983:992	15 samples per replicate of each treatment	983:1024	A total of 210 samples (15 samples per replicate of each treatment) were collected from cheese-making procedure and aging period.					
34998558	4	71	theme	composition	757:767	arg1	changes					769:775	microbial and physicochemical composition changes	727:775	microbial and physicochemical composition changes	727:775	This research focuses on the addition of fructooligosaccharides (FOS) or inulin to the Cheddar cheese-making process to evaluate the effects on microbial and physicochemical composition changes.					
34998558	10	72	theme	Lactobacillaceae	1912:1927	arg1	group					1903:1907	an unidentified group	1887:1907	an unidentified group of Lactobacillaceae, which emerged after 90 d of aging	1887:1962	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	10	72	theme	Lactobacillaceae	1912:1927	arg1	Lactobacillaceae					1912:1927	Lactobacillaceae	1912:1927	Lactobacillaceae	1912:1927	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	9	73	theme	control	1686:1692	arg1	groups					1694:1699	the control groups	1682:1699	the control groups	1682:1699	Fructooligosaccharides supplemented cheeses showed similar chemical properties to the control cheese, whereas inulin-supplemented cheeses exhibited a significantly higher moisture content than FOS and the control groups.					
34998558	3	74	theme	lactic	426:431	arg1	bacteria					438:445	lactic acid bacteria	426:445	lactic acid bacteria in cheese	426:455	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	11	75	theme	cheese	2046:2051	arg1	supplementation					2019:2033	prebiotic supplementation	2009:2033	prebiotic supplementation of Cheddar cheese	2009:2051	In conclusion, this study demonstrates that prebiotic supplementation of Cheddar cheese results in differing microbial and chemical characteristics.					
34998558	5	76	theme	Cheddar	795:801	arg1	cheese					803:808	Laboratory-scale Cheddar cheese	778:808	Laboratory-scale Cheddar cheese produced in 2	778:822	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	3	77	used	used	397:400	arg2	source					415:420	an energy source	405:420	an energy source for lactic acid bacteria in cheese	405:455	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	3	77	used	used	397:400	arg2	Prebiotics					379:388	Prebiotics	379:388	Prebiotics	379:388	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	3	77	used	used	397:400	arg2	potential					509:517	the potential	505:517	the potential for value-added foods, with a more stable probiotic population	505:580	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	11	78	theme	chemical	2088:2095	arg1	characteristics					2097:2111	microbial and chemical characteristics	2074:2111	microbial and chemical characteristics	2074:2111	In conclusion, this study demonstrates that prebiotic supplementation of Cheddar cheese results in differing microbial and chemical characteristics.					
34998558	8	79	theme	8.48	1436:1439	arg1	±					1441:1441	8.48 ± 0.06	1436:1446	8.48 ± 0.06	1436:1446	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	11	80	theme	microbial	2074:2082	arg1	characteristics					2097:2111	microbial and chemical characteristics	2074:2111	microbial and chemical characteristics	2074:2111	In conclusion, this study demonstrates that prebiotic supplementation of Cheddar cheese results in differing microbial and chemical characteristics.					
34998558	10	81	theme	inulin	1771:1776	arg1	%					1769:1769	2% inulin	1768:1776	2% inulin	1768:1776	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
34998558	2	82	theme	physiochemical	324:337	arg1	characteristics					339:353	physiochemical characteristics	324:353	physiochemical characteristics	324:353	Microorganisms within this fat, protein, and water matrix are largely responsible for physiochemical characteristics and associated quality.					
34998558	4	83	from	effects	716:722	arg1	changes					769:775	microbial and physicochemical composition changes	727:775	microbial and physicochemical composition changes	727:775	This research focuses on the addition of fructooligosaccharides (FOS) or inulin to the Cheddar cheese-making process to evaluate the effects on microbial and physicochemical composition changes.					
34998558	6	84	theme	cheese-making	1047:1059	arg1	procedure					1061:1069	cheese-making procedure	1047:1069	cheese-making procedure	1047:1069	A total of 210 samples (15 samples per replicate of each treatment) were collected from cheese-making procedure and aging period.					
34998558	8	85	theme	standard	1317:1324	arg1	deviation					1326:1334	± standard deviation	1315:1334	± standard deviation	1315:1334	The prevalence of lactic acid bacteria (log cfu/g) in Cheddar cheese supplemented with FOS (6.34 ± 0.11 and 8.99 ± 0.46; ± standard deviation) or inulin (6.02 ± 0.79 and 9.08 ± 1.00) was significantly higher than the control (5.84 ± 0.27 and 8.48 ± 0.06) in whey and curd, respectively.					
34998558	3	86	theme	microbial	473:481	arg1	community					483:491	the microbial community	469:491	the microbial community	469:491	Prebiotics can be used as an energy source for lactic acid bacteria in cheese by altering the microbial community and provide the potential for value-added foods, with a more stable probiotic population.					
34998558	1	87	theme	cheese	222:227	arg1	quality					229:235	cheese quality	222:235	cheese quality	222:235	Microbial and chemical properties of cheese is crucial in the dairy industry to understand their effects on cheese quality.					
34998558	5	88	theme	milk	953:956	arg1	L					923:923	18 L	920:923	18 L of commercially pasteurized milk	920:956	Laboratory-scale Cheddar cheese produced in 2 replicates was supplemented with 0 (control), 0.5, 1.0, and 2.0% (wt/wt) of FOS or inulin using 18 L of commercially pasteurized milk.					
34998558	10	89	theme	FOS-supplemented	1785:1800	arg1	cheeses					1802:1808	2% inulin and 2% FOS-supplemented cheeses	1768:1808	2% inulin and 2% FOS-supplemented cheeses	1768:1808	Streptococcus and Lactococcus were predominant in all cheeses and 2% inulin and 2% FOS-supplemented cheeses possessed significant amounts of nonstarter lactic acid bacteria found to be an unidentified group of Lactobacillaceae, which emerged after 90 d of aging.					
35063480	1	0	theme	ulcerative	294:303	arg1	colitis					305:311	ulcerative colitis	294:311	dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice	257:321	Chitin derivatives (CDs), including chitosan (CS), chitooligosaccharides (COS), and glucosamine (GlcN), were administrated in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice.					
35063480	1	0	theme	ulcerative	294:303	arg1	UC					314:315	UC	314:315	UC	314:315	Chitin derivatives (CDs), including chitosan (CS), chitooligosaccharides (COS), and glucosamine (GlcN), were administrated in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice.					
35063480	6	1	theme	relative	999:1006	arg1	abundance					1008:1016	relative abundance	999:1016	relative abundance of gut microbiota	999:1034	CD treatments increased relative abundance of gut microbiota, modulated its composition, and increased the concentrations of SCFAs.					
35063480	1	2	theme	colitis	305:311	arg1	mice					318:321	dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice	257:321	dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice	257:321	Chitin derivatives (CDs), including chitosan (CS), chitooligosaccharides (COS), and glucosamine (GlcN), were administrated in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice.					
35063480	4	3	theme	proteins	740:747	arg1	expression					711:720	expression	711:720	expression of tight junction proteins	711:747	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
35063480	4	4	theme	IL-10	593:597	arg1	level					599:603	IL-10 level	593:603	IL-10 level	593:603	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
35063480	0	5	theme	barrier	113:119	arg1	function					121:128	intestinal barrier function	102:128	intestinal barrier function	102:128	Chitin derivatives ameliorate DSS-induced ulcerative colitis by changing gut microbiota and restoring intestinal barrier function.					
35063480	5	6	theme	TLR-4	873:877	arg1	level					898:902	TLR-4 mRNA transcription level	873:902	TLR-4 mRNA transcription level	873:902	CDs (except GlcNL) significantly upregulated IκB-α level, and downregulated p65 and p38 phosphory lation and TLR-4 mRNA transcription level, indicating inhibition of TRL-4/NF-κB/MAPK signaling pathway activity.					
35063480	0	7	theme	intestinal	102:111	arg1	function					121:128	intestinal barrier function	102:128	intestinal barrier function	102:128	Chitin derivatives ameliorate DSS-induced ulcerative colitis by changing gut microbiota and restoring intestinal barrier function.					
35063480	5	8	theme	p38	848:850	arg1	lation					862:867	p38 phosphory lation	848:867	p38 phosphory lation	848:867	CDs (except GlcNL) significantly upregulated IκB-α level, and downregulated p65 and p38 phosphory lation and TLR-4 mRNA transcription level, indicating inhibition of TRL-4/NF-κB/MAPK signaling pathway activity.					
35063480	7	9	theme	ameliorative	1147:1158	arg1	effect					1160:1165	an ameliorative effect	1144:1165	an ameliorative effect	1144:1165	Our findings indicate that CDs exert an ameliorative effect on UC by change of gut microbiota composition and restoration of intestinal barrier function.					
35063480	4	10	theme	TNF-α	637:641	arg1	levels					614:619	levels	614:619	levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase	614:695	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
35063480	2	11	theme	GlcNL	442:446	arg1	group					448:452	GlcNL group	442:452	GlcNL group	442:452	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	5	12	theme	phosphory	852:860	arg1	lation					862:867	p38 phosphory lation	848:867	p38 phosphory lation	848:867	CDs (except GlcNL) significantly upregulated IκB-α level, and downregulated p65 and p38 phosphory lation and TLR-4 mRNA transcription level, indicating inhibition of TRL-4/NF-κB/MAPK signaling pathway activity.					
35063480	7	13	theme	function	1251:1258	arg1	change					1176:1181	change	1176:1181	change of gut microbiota composition	1176:1211	Our findings indicate that CDs exert an ameliorative effect on UC by change of gut microbiota composition and restoration of intestinal barrier function.					
35063480	7	13	theme	function	1251:1258	arg1	restoration					1217:1227	restoration	1217:1227	restoration of intestinal barrier function	1217:1258	Our findings indicate that CDs exert an ameliorative effect on UC by change of gut microbiota composition and restoration of intestinal barrier function.					
35063480	7	14	theme	intestinal	1232:1241	arg1	function					1251:1258	intestinal barrier function	1232:1258	intestinal barrier function	1232:1258	Our findings indicate that CDs exert an ameliorative effect on UC by change of gut microbiota composition and restoration of intestinal barrier function.					
35063480	4	15	theme	myeloperoxidase	644:658	arg1	levels					614:619	levels	614:619	levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase	614:695	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
35063480	6	16	theme	CD	975:976	arg1	treatments					978:987	CD treatments	975:987	CD treatments	975:987	CD treatments increased relative abundance of gut microbiota, modulated its composition, and increased the concentrations of SCFAs.					
35063480	4	17	theme	junction	731:738	arg1	proteins					740:747	tight junction proteins	725:747	tight junction proteins	725:747	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
35063480	5	18	theme	mRNA	879:882	arg1	level					898:902	TLR-4 mRNA transcription level	873:902	TLR-4 mRNA transcription level	873:902	CDs (except GlcNL) significantly upregulated IκB-α level, and downregulated p65 and p38 phosphory lation and TLR-4 mRNA transcription level, indicating inhibition of TRL-4/NF-κB/MAPK signaling pathway activity.					
35063480	0	19	theme	Chitin	0:5	arg1	derivatives					7:17	Chitin derivatives	0:17	Chitin derivatives	0:17	Chitin derivatives ameliorate DSS-induced ulcerative colitis by changing gut microbiota and restoring intestinal barrier function.					
35063480	4	20	theme	tight	725:729	arg1	proteins					740:747	tight junction proteins	725:747	tight junction proteins	725:747	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
35063480	4	21	theme	IL-1β	624:628	arg1	levels					614:619	levels	614:619	levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase	614:695	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
35063480	2	22	theme	UC	324:325	arg1	intake					375:380	reduced food intake	362:380	reduced food intake	362:380	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	2	22	theme	UC	324:325	arg1	index					414:418	increased disease activity index	387:418	increased disease activity index	387:418	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	2	22	theme	UC	324:325	arg1	loss					356:359	body weight loss	344:359	body weight loss	344:359	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	2	22	theme	UC	324:325	arg1	symptoms					327:334	UC symptoms	324:334	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index	324:418	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	0	23	theme	DSS-induced	30:40	arg1	colitis					53:59	DSS-induced ulcerative colitis	30:59	DSS-induced ulcerative colitis	30:59	Chitin derivatives ameliorate DSS-induced ulcerative colitis by changing gut microbiota and restoring intestinal barrier function.					
35063480	4	24	theme	oxide	682:686	arg1	synthase					688:695	inducible nitric oxide synthase	665:695	inducible nitric oxide synthase	665:695	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
35063480	7	25	theme	composition	1201:1211	arg1	change					1176:1181	change	1176:1181	change of gut microbiota composition	1176:1211	Our findings indicate that CDs exert an ameliorative effect on UC by change of gut microbiota composition and restoration of intestinal barrier function.					
35063480	7	25	theme	composition	1201:1211	arg1	restoration					1217:1227	restoration	1217:1227	restoration of intestinal barrier function	1217:1258	Our findings indicate that CDs exert an ameliorative effect on UC by change of gut microbiota composition and restoration of intestinal barrier function.					
35063480	2	26	theme	reduced	362:368	arg1	intake					375:380	reduced food intake	362:380	reduced food intake	362:380	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	5	27	theme	activity	965:972	arg1	inhibition					916:925	inhibition	916:925	inhibition of TRL-4/NF-κB/MAPK signaling pathway activity	916:972	CDs (except GlcNL) significantly upregulated IκB-α level, and downregulated p65 and p38 phosphory lation and TLR-4 mRNA transcription level, indicating inhibition of TRL-4/NF-κB/MAPK signaling pathway activity.					
35063480	4	28	theme	IL-6	631:634	arg1	levels					614:619	levels	614:619	levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase	614:695	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
35063480	2	29	theme	activity	405:412	arg1	index					414:418	increased disease activity index	387:418	increased disease activity index	387:418	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	7	30	theme	microbiota	1190:1199	arg1	composition					1201:1211	gut microbiota composition	1186:1211	gut microbiota composition	1186:1211	Our findings indicate that CDs exert an ameliorative effect on UC by change of gut microbiota composition and restoration of intestinal barrier function.					
35063480	7	31	theme	gut	1186:1188	arg1	composition					1201:1211	gut microbiota composition	1186:1211	gut microbiota composition	1186:1211	Our findings indicate that CDs exert an ameliorative effect on UC by change of gut microbiota composition and restoration of intestinal barrier function.					
35063480	6	32	theme	SCFAs	1100:1104	arg1	concentrations					1082:1095	the concentrations	1078:1095	the concentrations of SCFAs	1078:1104	CD treatments increased relative abundance of gut microbiota, modulated its composition, and increased the concentrations of SCFAs.					
35063480	2	33	theme	weight	349:354	arg1	loss					356:359	body weight loss	344:359	body weight loss	344:359	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	0	34	theme	ulcerative	42:51	arg1	colitis					53:59	DSS-induced ulcerative colitis	30:59	DSS-induced ulcerative colitis	30:59	Chitin derivatives ameliorate DSS-induced ulcerative colitis by changing gut microbiota and restoring intestinal barrier function.					
35063480	2	35	theme	disease	397:403	arg1	index					414:418	increased disease activity index	387:418	increased disease activity index	387:418	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	2	36	theme	food	370:373	arg1	intake					375:380	reduced food intake	362:380	reduced food intake	362:380	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	2	37	theme	body	344:347	arg1	loss					356:359	body weight loss	344:359	body weight loss	344:359	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	2	38	theme	increased	387:395	arg1	index					414:418	increased disease activity index	387:418	increased disease activity index	387:418	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	5	39	theme	TRL-4/NF-κB/MAPK	930:945	arg1	activity					965:972	TRL-4/NF-κB/MAPK signaling pathway activity	930:972	TRL-4/NF-κB/MAPK signaling pathway activity	930:972	CDs (except GlcNL) significantly upregulated IκB-α level, and downregulated p65 and p38 phosphory lation and TLR-4 mRNA transcription level, indicating inhibition of TRL-4/NF-κB/MAPK signaling pathway activity.					
35063480	3	40	theme	strong	485:490	arg1	effect					503:508	a strong protective effect	483:508	a strong protective effect	483:508	CDs (except GlcNL) exerted a strong protective effect on colon length and colonic structure.					
35063480	1	41	theme	Chitin	131:136	arg1	glucosamine					215:225	glucosamine	215:225	glucosamine (GlcN)	215:232	Chitin derivatives (CDs), including chitosan (CS), chitooligosaccharides (COS), and glucosamine (GlcN), were administrated in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice.					
35063480	1	41	theme	Chitin	131:136	arg1	CDs					151:153	CDs	151:153	CDs	151:153	Chitin derivatives (CDs), including chitosan (CS), chitooligosaccharides (COS), and glucosamine (GlcN), were administrated in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice.					
35063480	1	41	theme	Chitin	131:136	arg1	derivatives					138:148	Chitin derivatives	131:148	Chitin derivatives (CDs)	131:154	Chitin derivatives (CDs), including chitosan (CS), chitooligosaccharides (COS), and glucosamine (GlcN), were administrated in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice.					
35063480	1	41	theme	Chitin	131:136	arg1	chitooligosaccharides					182:202	chitooligosaccharides	182:202	chitooligosaccharides (COS)	182:208	Chitin derivatives (CDs), including chitosan (CS), chitooligosaccharides (COS), and glucosamine (GlcN), were administrated in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice.					
35063480	1	41	theme	Chitin	131:136	arg1	chitosan					167:174	chitosan	167:174	chitosan (CS)	167:179	Chitin derivatives (CDs), including chitosan (CS), chitooligosaccharides (COS), and glucosamine (GlcN), were administrated in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice.					
35063480	3	42	theme	protective	492:501	arg1	effect					503:508	a strong protective effect	483:508	a strong protective effect	483:508	CDs (except GlcNL) exerted a strong protective effect on colon length and colonic structure.					
35063480	5	43	theme	transcription	884:896	arg1	level					898:902	TLR-4 mRNA transcription level	873:902	TLR-4 mRNA transcription level	873:902	CDs (except GlcNL) significantly upregulated IκB-α level, and downregulated p65 and p38 phosphory lation and TLR-4 mRNA transcription level, indicating inhibition of TRL-4/NF-κB/MAPK signaling pathway activity.					
35063480	4	44	with	Treatment	549:557	arg1	CDs					564:566	CDs	564:566	CDs	564:566	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
35063480	4	45	theme	inducible	665:673	arg1	synthase					688:695	inducible nitric oxide synthase	665:695	inducible nitric oxide synthase	665:695	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
35063480	4	46	theme	synthase	688:695	arg1	levels					614:619	levels	614:619	levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase	614:695	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
35063480	5	47	theme	IκB-α	809:813	arg1	level					815:819	IκB-α level	809:819	IκB-α level	809:819	CDs (except GlcNL) significantly upregulated IκB-α level, and downregulated p65 and p38 phosphory lation and TLR-4 mRNA transcription level, indicating inhibition of TRL-4/NF-κB/MAPK signaling pathway activity.					
35063480	2	48	dep	relieved	425:432	arg1	group					448:452	GlcNL group	442:452	GlcNL group	442:452	UC symptoms such as body weight loss, reduced food intake, and increased disease activity index were relieved (except GlcNL group).					
35063480	5	49	theme	signaling	947:955	arg1	activity					965:972	TRL-4/NF-κB/MAPK signaling pathway activity	930:972	TRL-4/NF-κB/MAPK signaling pathway activity	930:972	CDs (except GlcNL) significantly upregulated IκB-α level, and downregulated p65 and p38 phosphory lation and TLR-4 mRNA transcription level, indicating inhibition of TRL-4/NF-κB/MAPK signaling pathway activity.					
35063480	3	50	theme	colonic	530:536	arg1	structure					538:546	colonic structure	530:546	colonic structure	530:546	CDs (except GlcNL) exerted a strong protective effect on colon length and colonic structure.					
35063480	6	51	theme	gut	1021:1023	arg1	microbiota					1025:1034	gut microbiota	1021:1034	gut microbiota	1021:1034	CD treatments increased relative abundance of gut microbiota, modulated its composition, and increased the concentrations of SCFAs.					
35063480	6	52	theme	microbiota	1025:1034	arg1	abundance					1008:1016	relative abundance	999:1016	relative abundance of gut microbiota	999:1034	CD treatments increased relative abundance of gut microbiota, modulated its composition, and increased the concentrations of SCFAs.					
35063480	5	53	theme	pathway	957:963	arg1	activity					965:972	TRL-4/NF-κB/MAPK signaling pathway activity	930:972	TRL-4/NF-κB/MAPK signaling pathway activity	930:972	CDs (except GlcNL) significantly upregulated IκB-α level, and downregulated p65 and p38 phosphory lation and TLR-4 mRNA transcription level, indicating inhibition of TRL-4/NF-κB/MAPK signaling pathway activity.					
35063480	3	54	theme	colon	513:517	arg1	length					519:524	colon length	513:524	colon length	513:524	CDs (except GlcNL) exerted a strong protective effect on colon length and colonic structure.					
35063480	7	55	theme	barrier	1243:1249	arg1	function					1251:1258	intestinal barrier function	1232:1258	intestinal barrier function	1232:1258	Our findings indicate that CDs exert an ameliorative effect on UC by change of gut microbiota composition and restoration of intestinal barrier function.					
35063480	0	56	theme	gut	73:75	arg1	microbiota					77:86	gut microbiota	73:86	gut microbiota	73:86	Chitin derivatives ameliorate DSS-induced ulcerative colitis by changing gut microbiota and restoring intestinal barrier function.					
35063480	1	57	theme	-induced	285:292	arg1	mice					318:321	dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice	257:321	dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice	257:321	Chitin derivatives (CDs), including chitosan (CS), chitooligosaccharides (COS), and glucosamine (GlcN), were administrated in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice.					
35063480	4	58	theme	nitric	675:680	arg1	synthase					688:695	inducible nitric oxide synthase	665:695	inducible nitric oxide synthase	665:695	Treatment with CDs (except GlcNL) increased IL-10 level, reduced levels of IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase, and enhanced expression of tight junction proteins significantly.					
37330844	2	0	theme	fermentation	763:774	arg1	48 h					738:741	48 h	738:741	48 h of in vitro colonic fermentation	738:774	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	0	1	from	activity	146:153	arg1	groups					125:130	target bacterial groups	108:130	target bacterial groups	108:130	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	11	2	theme	short-chain	1566:1576	arg1	acid					1584:1587	short-chain fatty acid	1566:1587	short-chain fatty acid production	1566:1598	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	1	3	theme	metabolic	393:401	arg1	activity					403:410	metabolic activity	393:410	metabolic activity	393:410	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	2	4	theme	bacterial	552:560	arg1	groups					562:567	different selected bacterial groups	533:567	different selected bacterial groups found as part of human intestinal microbiota	533:612	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	0	5	theme	colonic	164:170	arg1	microbiota					172:181	human colonic microbiota	158:181	human colonic microbiota	158:181	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	10	6	theme	prebiotic	1304:1312	arg1	indexes					1314:1320	high positive prebiotic indexes	1290:1320	high positive prebiotic indexes (>3.61)	1290:1328	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	10	6	theme	prebiotic	1304:1312	arg1	>3.61					1323:1327	>3.61	1323:1327	>3.61	1323:1327	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	12	7	theme	novel	1964:1968	arg1	ingredients					1996:2006	novel and sustainable prebiotic ingredients	1964:2006	novel and sustainable prebiotic ingredients	1964:2006	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	2	8	theme	human	586:590	arg1	microbiota					603:612	human intestinal microbiota	586:612	human intestinal microbiota	586:612	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	11	9	theme	microbiota	1508:1517	arg1	activity					1482:1489	the metabolic activity	1468:1489	the metabolic activity of human colonic microbiota	1468:1517	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	11	10	from	alterations	1601:1611	arg1	contents					1634:1641	phenolic compound contents	1616:1641	phenolic compound contents	1616:1641	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	2	11	theme	phenolic	671:678	arg1	compounds					680:688	phenolic compounds	671:688	phenolic compounds	671:688	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	0	12	theme	novel	194:198	arg1	ingredients					226:236	novel and sustainable prebiotic ingredients	194:236	novel and sustainable prebiotic ingredients	194:236	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	11	13	theme	human	1494:1498	arg1	microbiota					1508:1517	human colonic microbiota	1494:1517	human colonic microbiota	1494:1517	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	12	14	theme	unconventional	1902:1915	arg1	parts					1933:1937	conventional and unconventional red beet edible parts	1885:1937	conventional and unconventional red beet edible parts	1885:1937	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	12	14	theme	unconventional	1902:1915	arg1	candidates					1943:1952	candidates	1943:1952	candidates to use as novel and sustainable prebiotic ingredients	1943:2006	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	2	15	theme	antioxidant	695:705	arg1	capacity					707:714	antioxidant capacity	695:714	antioxidant capacity	695:714	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	0	16	theme	target	108:113	arg1	groups					125:130	target bacterial groups	108:130	target bacterial groups	108:130	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	11	17	from	pH	1543:1544	arg1	contents					1634:1641	phenolic compound contents	1616:1641	phenolic compound contents	1616:1641	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	12	18	theme	beet	1921:1924	arg1	parts					1933:1937	conventional and unconventional red beet edible parts	1885:1937	conventional and unconventional red beet edible parts	1885:1937	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	12	18	theme	beet	1921:1924	arg1	candidates					1943:1952	candidates	1943:1952	candidates to use as novel and sustainable prebiotic ingredients	1943:2006	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	2	19	theme	different	533:541	arg1	groups					562:567	different selected bacterial groups	533:567	different selected bacterial groups found as part of human intestinal microbiota	533:612	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	1	20	theme	target	365:370	arg1	microbiota					429:438	human colonic microbiota	415:438	human colonic microbiota	415:438	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	20	theme	target	365:370	arg1	groups					382:387	target bacterial groups	365:387	target bacterial groups	365:387	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	3	21	theme	gastrointestinal	820:835	arg1	digestion					837:845	simulated gastrointestinal digestion	810:845	simulated gastrointestinal digestion	810:845	FDBR and FDBSL were submitted to simulated gastrointestinal digestion and freeze-dried prior to use in colonic fermentation.					
37330844	11	22	theme	colonic	1696:1702	arg1	fermentation					1704:1715	colonic fermentation	1696:1715	colonic fermentation	1696:1715	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	0	23	theme	metabolic	136:144	arg1	activity					146:153	metabolic activity	136:153	metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients	136:236	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	11	24	from	maintenance	1648:1658	arg1	contents					1634:1641	phenolic compound contents	1616:1641	phenolic compound contents	1616:1641	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	11	25	theme	capacity	1680:1687	arg1	alterations					1601:1611	alterations	1601:1611	alterations in phenolic compound contents	1601:1641	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	11	25	theme	capacity	1680:1687	arg1	pH					1543:1544	decreased pH	1533:1544	decreased pH	1533:1544	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	11	25	theme	capacity	1680:1687	arg1	consumption					1553:1563	sugar consumption	1547:1563	sugar consumption	1547:1563	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	11	25	theme	capacity	1680:1687	arg1	maintenance					1648:1658	maintenance	1648:1658	maintenance of high antioxidant capacity during colonic fermentation	1648:1715	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	11	25	theme	capacity	1680:1687	arg1	production					1589:1598	short-chain fatty acid production	1566:1598	short-chain fatty acid production	1566:1598	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	0	26	theme	parts	68:72	arg1	effects					18:24	the effects	14:24	the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups	14:130	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	0	26	theme	parts	68:72	arg1	activity					146:153	metabolic activity	136:153	metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients	136:236	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	3	27	theme	simulated	810:818	arg1	digestion					837:845	simulated gastrointestinal digestion	810:845	simulated gastrointestinal digestion	810:845	FDBR and FDBSL were submitted to simulated gastrointestinal digestion and freeze-dried prior to use in colonic fermentation.					
37330844	0	28	from	effects	18:24	arg1	groups					125:130	target bacterial groups	108:130	target bacterial groups	108:130	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	10	29	theme	selective	1370:1378	arg1	effects					1392:1398	selective stimulatory effects	1370:1398	selective stimulatory effects on beneficial intestinal bacterial groups	1370:1440	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	2	30	theme	short-chain	647:657	arg1	acid					665:668	short-chain fatty acid	647:668	short-chain fatty acid	647:668	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	0	31	theme	red	77:79	arg1	beet					81:84	red beet	77:84	red beet (Beta vulgaris L.)	77:103	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	0	31	theme	red	77:79	arg1	L.					101:102	Beta vulgaris L.	87:102	Beta vulgaris L.	87:102	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	1	32	theme	freeze-dried	316:327	arg1	stem					338:341	freeze-dried red beet stem and leaves	316:352	stem	338:341	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	32	theme	freeze-dried	316:327	arg1	FDBSL					355:359	FDBSL	355:359	FDBSL	355:359	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	0	33	theme	prebiotic	216:224	arg1	ingredients					226:236	novel and sustainable prebiotic ingredients	194:236	novel and sustainable prebiotic ingredients	194:236	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	1	34	theme	beet	333:336	arg1	stem					338:341	freeze-dried red beet stem and leaves	316:352	stem	338:341	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	34	theme	beet	333:336	arg1	FDBSL					355:359	FDBSL	355:359	FDBSL	355:359	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	10	35	theme	beneficial	1403:1412	arg1	groups					1435:1440	beneficial intestinal bacterial groups	1403:1440	beneficial intestinal bacterial groups	1403:1440	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	2	36	theme	intestinal	592:601	arg1	microbiota					603:612	human intestinal microbiota	586:612	human intestinal microbiota	586:612	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	6	37	dep	%	1008:1008	arg1	Bifidobacterium					1015:1029	Bifidobacterium	1015:1029	Bifidobacterium	1015:1029	(3.64-7.60%) and Bifidobacterium spp.					
37330844	0	38	theme	vulgaris	92:99	arg1	beet					81:84	red beet	77:84	red beet (Beta vulgaris L.)	77:103	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	0	38	theme	vulgaris	92:99	arg1	L.					101:102	Beta vulgaris L.	87:102	Beta vulgaris L.	87:102	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	12	39	theme	intestinal	1846:1855	arg1	microbiota					1857:1866	human intestinal microbiota	1840:1866	human intestinal microbiota	1840:1866	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	9	40	theme	colonic	1249:1255	arg1	fermentation					1257:1268	colonic fermentation	1249:1268	colonic fermentation	1249:1268	(9.56-4.18%), Clostridium histolyticum (1.62-1.15%), and Eubacterium rectale/Clostridium coccoides (2.33-1.49%) during 48 h of colonic fermentation.					
37330844	10	41	theme	positive	1295:1302	arg1	indexes					1314:1320	high positive prebiotic indexes	1290:1320	high positive prebiotic indexes (>3.61)	1290:1328	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	10	41	theme	positive	1295:1302	arg1	>3.61					1323:1327	>3.61	1323:1327	>3.61	1323:1327	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	1	42	theme	freeze-dried	278:289	arg1	root					300:303	freeze-dried red beet root	278:303	freeze-dried red beet root (FDBR)	278:310	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	42	theme	freeze-dried	278:289	arg1	FDBR					306:309	FDBR	306:309	FDBR	306:309	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	12	43	theme	metabolic	1818:1826	arg1	activity					1828:1835	metabolic activity	1818:1835	metabolic activity	1818:1835	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	10	44	theme	bacterial	1425:1433	arg1	groups					1435:1440	beneficial intestinal bacterial groups	1403:1440	beneficial intestinal bacterial groups	1403:1440	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	7	45	theme	Bacteroides	1089:1099	arg1	spp					1101:1103	Bacteroides spp	1089:1103	Bacteroides spp	1089:1103	(2.76-5.78%) and decreased the relative abundance of Bacteroides spp.					
37330844	1	46	theme	beet	295:298	arg1	root					300:303	freeze-dried red beet root	278:303	freeze-dried red beet root (FDBR)	278:310	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	46	theme	beet	295:298	arg1	FDBR					306:309	FDBR	306:309	FDBR	306:309	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	0	47	theme	conventional	29:40	arg1	parts					68:72	conventional and unconventional edible parts	29:72	conventional and unconventional edible parts of red beet (Beta vulgaris L.)	29:103	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	4	48	theme	Lactobacillus	961:973	arg1	spp					975:977	Lactobacillus spp	961:977	Lactobacillus spp	961:977	FDBR and FDBSL overall increased the relative abundance of Lactobacillus spp.					
37330844	0	49	theme	unconventional	46:59	arg1	parts					68:72	conventional and unconventional edible parts	29:72	conventional and unconventional edible parts of red beet (Beta vulgaris L.)	29:103	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	10	50	contain	had	1286:1288	arg1	FDBSL					1280:1284	FDBSL	1280:1284	FDBSL	1280:1284	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	10	50	contain	had	1286:1288	arg1	FDBR					1271:1274	FDBR	1271:1274	FDBR	1271:1274	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	10	50	contain	had	1286:1288	arg2	>3.61					1323:1327	>3.61	1323:1327	>3.61	1323:1327	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	10	50	contain	had	1286:1288	arg2	indexes					1314:1320	high positive prebiotic indexes	1290:1320	high positive prebiotic indexes (>3.61)	1290:1328	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	2	51	theme	in	746:747	arg1	fermentation					763:774	in vitro colonic fermentation	746:774	in vitro colonic fermentation	746:774	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	1	52	theme	colonic	421:427	arg1	microbiota					429:438	human colonic microbiota	415:438	human colonic microbiota	415:438	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	0	53	theme	edible	61:66	arg1	parts					68:72	conventional and unconventional edible parts	29:72	conventional and unconventional edible parts of red beet (Beta vulgaris L.)	29:103	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	0	54	theme	human	158:162	arg1	microbiota					172:181	human colonic microbiota	158:181	human colonic microbiota	158:181	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	11	55	theme	sugar	1547:1551	arg1	consumption					1553:1563	sugar consumption	1547:1563	sugar consumption	1547:1563	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	12	56	theme	prebiotic	1986:1994	arg1	ingredients					1996:2006	novel and sustainable prebiotic ingredients	1964:2006	novel and sustainable prebiotic ingredients	1964:2006	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	2	57	theme	groups	562:567	arg1	abundance					520:528	the relative abundance	507:528	the relative abundance of different selected bacterial groups found as part of human intestinal microbiota	507:612	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	2	58	theme	colonic	755:761	arg1	fermentation					763:774	in vitro colonic fermentation	746:774	in vitro colonic fermentation	746:774	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	11	59	theme	fatty	1578:1582	arg1	acid					1584:1587	short-chain fatty acid	1566:1587	short-chain fatty acid production	1566:1598	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	0	60	theme	microbiota	172:181	arg1	effects					18:24	the effects	14:24	the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups	14:130	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	0	60	theme	microbiota	172:181	arg1	activity					146:153	metabolic activity	136:153	metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients	136:236	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	2	61	theme	selected	543:550	arg1	groups					562:567	different selected bacterial groups	533:567	different selected bacterial groups found as part of human intestinal microbiota	533:612	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	11	62	theme	phenolic	1616:1623	arg1	contents					1634:1641	phenolic compound contents	1616:1641	phenolic compound contents	1616:1641	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	1	63	theme	human	415:419	arg1	microbiota					429:438	human colonic microbiota	415:438	human colonic microbiota	415:438	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	10	64	theme	colonic	1337:1343	arg1	fermentation					1345:1356	colonic fermentation	1337:1356	colonic fermentation	1337:1356	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	1	65	from	effects	267:273	arg1	microbiota					429:438	human colonic microbiota	415:438	human colonic microbiota	415:438	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	65	from	effects	267:273	arg1	groups					382:387	target bacterial groups	365:387	target bacterial groups	365:387	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	65	from	effects	267:273	arg1	activity					403:410	metabolic activity	393:410	metabolic activity	393:410	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	66	theme	microbiota	429:438	arg1	microbiota					429:438	human colonic microbiota	415:438	human colonic microbiota	415:438	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	66	theme	microbiota	429:438	arg1	groups					382:387	target bacterial groups	365:387	target bacterial groups	365:387	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	66	theme	microbiota	429:438	arg1	activity					403:410	metabolic activity	393:410	metabolic activity	393:410	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	11	67	from	consumption	1553:1563	arg1	contents					1634:1641	phenolic compound contents	1616:1641	phenolic compound contents	1616:1641	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	12	68	theme	edible	1926:1931	arg1	parts					1933:1937	conventional and unconventional red beet edible parts	1885:1937	conventional and unconventional red beet edible parts	1885:1937	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	12	68	theme	edible	1926:1931	arg1	candidates					1943:1952	candidates	1943:1952	candidates to use as novel and sustainable prebiotic ingredients	1943:2006	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	2	69	dep	in	746:747	arg1	vitro					749:753	vitro	749:753	vitro	749:753	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	7	70	dep	decreased	1053:1061	arg1	%					1046:1046	2.76-5.78%	1037:1046	2.76-5.78%	1037:1046	(2.76-5.78%) and decreased the relative abundance of Bacteroides spp.					
37330844	11	71	theme	colonic	1500:1506	arg1	microbiota					1508:1517	human colonic microbiota	1494:1517	human colonic microbiota	1494:1517	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	0	72	theme	bacterial	115:123	arg1	groups					125:130	target bacterial groups	108:130	target bacterial groups	108:130	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	10	73	from	effects	1392:1398	arg1	groups					1435:1440	beneficial intestinal bacterial groups	1403:1440	beneficial intestinal bacterial groups	1403:1440	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	3	74	from	use	873:875	arg1	fermentation					888:899	colonic fermentation	880:899	colonic fermentation	880:899	FDBR and FDBSL were submitted to simulated gastrointestinal digestion and freeze-dried prior to use in colonic fermentation.					
37330844	12	75	theme	red	1917:1919	arg1	parts					1933:1937	conventional and unconventional red beet edible parts	1885:1937	conventional and unconventional red beet edible parts	1885:1937	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	12	75	theme	red	1917:1919	arg1	candidates					1943:1952	candidates	1943:1952	candidates to use as novel and sustainable prebiotic ingredients	1943:2006	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	11	76	theme	antioxidant	1668:1678	arg1	capacity					1680:1687	high antioxidant capacity	1663:1687	high antioxidant capacity	1663:1687	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	1	77	theme	bacterial	372:380	arg1	microbiota					429:438	human colonic microbiota	415:438	human colonic microbiota	415:438	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	77	theme	bacterial	372:380	arg1	groups					382:387	target bacterial groups	365:387	target bacterial groups	365:387	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	2	78	theme	relative	511:518	arg1	abundance					520:528	the relative abundance	507:528	the relative abundance of different selected bacterial groups found as part of human intestinal microbiota	507:612	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	2	79	theme	fatty	659:663	arg1	acid					665:668	short-chain fatty acid	647:668	short-chain fatty acid	647:668	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	0	80	theme	beet	81:84	arg1	parts					68:72	conventional and unconventional edible parts	29:72	conventional and unconventional edible parts of red beet (Beta vulgaris L.)	29:103	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	9	81	dep	histolyticum	1148:1159	arg1	%					1132:1132	9.56-4.18%	1123:1132	9.56-4.18%	1123:1132	(9.56-4.18%), Clostridium histolyticum (1.62-1.15%), and Eubacterium rectale/Clostridium coccoides (2.33-1.49%) during 48 h of colonic fermentation.					
37330844	0	82	theme	Beta	87:90	arg1	beet					81:84	red beet	77:84	red beet (Beta vulgaris L.)	77:103	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	0	82	theme	Beta	87:90	arg1	L.					101:102	Beta vulgaris L.	87:102	Beta vulgaris L.	87:102	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	1	83	theme	red	329:331	arg1	stem					338:341	freeze-dried red beet stem and leaves	316:352	stem	338:341	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	83	theme	red	329:331	arg1	FDBSL					355:359	FDBSL	355:359	FDBSL	355:359	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	12	84	theme	conventional	1885:1896	arg1	parts					1933:1937	conventional and unconventional red beet edible parts	1885:1937	conventional and unconventional red beet edible parts	1885:1937	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	12	84	theme	conventional	1885:1896	arg1	candidates					1943:1952	candidates	1943:1952	candidates to use as novel and sustainable prebiotic ingredients	1943:2006	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	0	85	theme	sustainable	204:214	arg1	ingredients					226:236	novel and sustainable prebiotic ingredients	194:236	novel and sustainable prebiotic ingredients	194:236	Investigating the effects of conventional and unconventional edible parts of red beet (Beta vulgaris L.) on target bacterial groups and metabolic activity of human colonic microbiota to produce novel and sustainable prebiotic ingredients.					
37330844	2	86	theme	FDBSL	477:481	arg1	capability					454:463	The capability	450:463	The capability of FDBR and FDBSL	450:481	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	2	87	theme	microbiota	603:612	arg1	part					578:581	part	578:581	part of human intestinal microbiota	578:612	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	1	88	theme	stem	338:341	arg1	effects					267:273	the effects	263:273	the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota	263:438	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	12	89	theme	microbiota	1857:1866	arg1	composition					1802:1812	composition	1802:1812	composition	1802:1812	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	12	89	theme	microbiota	1857:1866	arg1	activity					1828:1835	metabolic activity	1818:1835	metabolic activity	1818:1835	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	12	89	theme	microbiota	1857:1866	arg1	parts					1933:1937	conventional and unconventional red beet edible parts	1885:1937	conventional and unconventional red beet edible parts	1885:1937	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	12	89	theme	microbiota	1857:1866	arg1	candidates					1943:1952	candidates	1943:1952	candidates to use as novel and sustainable prebiotic ingredients	1943:2006	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	2	90	theme	FDBR	468:471	arg1	capability					454:463	The capability	450:463	The capability of FDBR and FDBSL	450:481	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	2	91	theme	pH	629:630	arg1	values					632:637	pH values	629:637	pH values	629:637	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	4	92	theme	spp	975:977	arg1	abundance					948:956	the relative abundance	935:956	the relative abundance of Lactobacillus spp	935:977	FDBR and FDBSL overall increased the relative abundance of Lactobacillus spp.					
37330844	10	93	theme	stimulatory	1380:1390	arg1	effects					1392:1398	selective stimulatory effects	1370:1398	selective stimulatory effects on beneficial intestinal bacterial groups	1370:1440	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	7	94	theme	spp	1101:1103	arg1	abundance					1076:1084	the relative abundance	1063:1084	the relative abundance of Bacteroides spp	1063:1103	(2.76-5.78%) and decreased the relative abundance of Bacteroides spp.					
37330844	9	95	theme	fermentation	1257:1268	arg1	48 h					1241:1244	48 h	1241:1244	48 h of colonic fermentation	1241:1268	(9.56-4.18%), Clostridium histolyticum (1.62-1.15%), and Eubacterium rectale/Clostridium coccoides (2.33-1.49%) during 48 h of colonic fermentation.					
37330844	3	96	theme	colonic	880:886	arg1	fermentation					888:899	colonic fermentation	880:899	colonic fermentation	880:899	FDBR and FDBSL were submitted to simulated gastrointestinal digestion and freeze-dried prior to use in colonic fermentation.					
37330844	1	97	theme	red	291:293	arg1	root					300:303	freeze-dried red beet root	278:303	freeze-dried red beet root (FDBR)	278:310	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	1	97	theme	red	291:293	arg1	FDBR					306:309	FDBR	306:309	FDBR	306:309	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	12	98	theme	human	1840:1844	arg1	microbiota					1857:1866	human intestinal microbiota	1840:1866	human intestinal microbiota	1840:1866	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	4	99	theme	relative	939:946	arg1	abundance					948:956	the relative abundance	935:956	the relative abundance of Lactobacillus spp	935:977	FDBR and FDBSL overall increased the relative abundance of Lactobacillus spp.					
37330844	10	100	theme	intestinal	1414:1423	arg1	groups					1435:1440	beneficial intestinal bacterial groups	1403:1440	beneficial intestinal bacterial groups	1403:1440	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	11	101	theme	compound	1625:1632	arg1	contents					1634:1641	phenolic compound contents	1616:1641	phenolic compound contents	1616:1641	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	11	102	theme	decreased	1533:1541	arg1	pH					1543:1544	decreased pH	1533:1544	decreased pH	1533:1544	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	7	103	theme	relative	1067:1074	arg1	abundance					1076:1084	the relative abundance	1063:1084	the relative abundance of Bacteroides spp	1063:1103	(2.76-5.78%) and decreased the relative abundance of Bacteroides spp.					
37330844	1	104	theme	root	300:303	arg1	effects					267:273	the effects	263:273	the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota	263:438	This study investigated the effects of freeze-dried red beet root (FDBR) and freeze-dried red beet stem and leaves (FDBSL) on target bacterial groups and metabolic activity of human colonic microbiota in vitro.					
37330844	11	105	from	production	1589:1598	arg1	contents					1634:1641	phenolic compound contents	1616:1641	phenolic compound contents	1616:1641	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	11	106	theme	high	1663:1666	arg1	capacity					1680:1687	high antioxidant capacity	1663:1687	high antioxidant capacity	1663:1687	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	12	107	theme	beneficial	1772:1781	arg1	alterations					1783:1793	beneficial alterations	1772:1793	beneficial alterations	1772:1793	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	2	108	from	alterations	492:502	arg1	abundance					520:528	the relative abundance	507:528	the relative abundance of different selected bacterial groups found as part of human intestinal microbiota	507:612	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	2	108	from	alterations	492:502	arg1	values					632:637	pH values	629:637	pH values	629:637	The capability of FDBR and FDBSL to cause alterations in the relative abundance of different selected bacterial groups found as part of human intestinal microbiota, as well as in pH values, sugar, short-chain fatty acid, phenolic compounds, and antioxidant capacity were evaluated during 48 h of in vitro colonic fermentation.					
37330844	10	109	theme	high	1290:1293	arg1	indexes					1314:1320	high positive prebiotic indexes	1290:1320	high positive prebiotic indexes (>3.61)	1290:1328	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	10	109	theme	high	1290:1293	arg1	>3.61					1323:1327	>3.61	1323:1327	>3.61	1323:1327	FDBR and FDBSL had high positive prebiotic indexes (>3.61) during colonic fermentation, indicating selective stimulatory effects on beneficial intestinal bacterial groups.					
37330844	12	110	theme	sustainable	1974:1984	arg1	ingredients					1996:2006	novel and sustainable prebiotic ingredients	1964:2006	novel and sustainable prebiotic ingredients	1964:2006	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	12	111	dep	composition	1802:1812	arg1	the					1798:1800	the	1798:1800	the	1798:1800	The results indicate that FDBR and FDBSL could induce beneficial alterations in the composition and metabolic activity of human intestinal microbiota, as well as that conventional and unconventional red beet edible parts are candidates to use as novel and sustainable prebiotic ingredients.					
37330844	11	112	theme	acid	1584:1587	arg1	production					1589:1598	short-chain fatty acid production	1566:1598	short-chain fatty acid production	1566:1598	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
37330844	11	113	theme	metabolic	1472:1480	arg1	activity					1482:1489	the metabolic activity	1468:1489	the metabolic activity of human colonic microbiota	1468:1517	FDBR and FDBSL increased the metabolic activity of human colonic microbiota, evidenced by decreased pH, sugar consumption, short-chain fatty acid production, alterations in phenolic compound contents, and maintenance of high antioxidant capacity during colonic fermentation.					
35223213	3	0	theme	pure	563:566	arg1	%					561:561	99.9% pure	557:566	99.9% pure	557:566	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	3	1	dep	hours	769:773	arg1	cm					797:798	5 × 10 cm	790:798	5 × 10 cm	790:798	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	2	2	theme	dry	508:510	arg1	matter					512:517	dry matter	508:517	dry matter (DM) of substrates	508:536	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	2	2	theme	dry	508:510	arg1	DM					520:521	DM	520:521	DM	520:521	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	5	3	theme	guideline	1209:1217	arg1	requirements					1180:1191	national research council (NRC) nutrient requirements	1139:1191	national research council (NRC) nutrient requirements of dairy cattle guideline	1139:1217	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	3	4	theme	serum	590:594	arg1	bottles					596:602	the serum bottles	586:602	the serum bottles	586:602	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	5	5	theme	SSB	1096:1098	arg1	treatments					1044:1053	The treatments	1040:1053	The treatments	1040:1053	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	5	5	theme	SSB	1096:1098	arg1	diet					1066:1069	basal diet	1060:1069	basal diet (control)	1060:1079	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	5	5	theme	SSB	1096:1098	arg1	DM					1090:1091	3.0% DM	1085:1091	3.0% DM of SSB	1085:1098	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	5	5	theme	SSB	1096:1098	arg1	control					1072:1078	control	1072:1078	control	1072:1078	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	1	6	theme	sugar	231:235	arg1	feeding					254:260	starch sugar by-product (SSB) feeding	224:260	starch sugar by-product (SSB) feeding	224:260	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	3	7	theme	Holstein	865:872	arg1	cows					874:877	two cannulated Holstein cows	850:877	two cannulated Holstein cows	850:877	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	2	8	theme	substrates	527:536	arg1	matter					512:517	dry matter	508:517	dry matter (DM) of substrates	508:536	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	2	8	theme	substrates	527:536	arg1	DM					520:521	DM	520:521	DM	520:521	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	7	9	dep	%	1499:1499	arg1	DM					1500:1501	DM	1500:1501	26.38 and 56.47%DM	1484:1501	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	5	10	theme	3.0	1085:1087	arg1	%					1088:1088	%	1088:1088	%	1088:1088	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	12	11	theme	3.0	1935:1937	arg1	%					1938:1938	%	1938:1938	%	1938:1938	CONCLUSION A diet containing 3.0% SSB could be fed to ruminants without adverse effects on rumen fermentation.					
35223213	2	12	theme	125	468:470	arg1	mL					472:473	mL	472:473	mL	472:473	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	4	13	dep	cows	918:921	arg1	months					936:941	60.5 ± 20.4 months	924:941	60.5 ± 20.4 months old	924:945	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	4	13	dep	cows	918:921	arg1	wieght					976:981	706.8 ± 3.4 kg initial body wieght	948:981	706.8 ± 3.4 kg initial body wieght	948:981	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	1	14	theme	dairy	341:345	arg1	cow					347:349	lactating dairy cow	331:349	lactating dairy cow	331:349	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	8	15	theme	total	1532:1536	arg1	ration					1544:1549	total mixed ration	1532:1549	total mixed ration (TMR)	1532:1555	As an increase SSB level in total mixed ration (TMR), there was a decrease in gas production at 0, 16, and 48 h (p < 0.05).					
35223213	8	15	theme	total	1532:1536	arg1	TMR					1552:1554	TMR	1552:1554	TMR	1552:1554	As an increase SSB level in total mixed ration (TMR), there was a decrease in gas production at 0, 16, and 48 h (p < 0.05).					
35223213	1	16	theme	study	186:190	arg1	purpose					163:169	The purpose	159:169	The purpose of the present study	159:190	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	12	17	theme	adverse	1978:1984	arg1	effects					1986:1992	adverse effects	1978:1992	adverse effects on rumen fermentation	1978:2014	CONCLUSION A diet containing 3.0% SSB could be fed to ruminants without adverse effects on rumen fermentation.					
35223213	3	18	dep	rate	714:717	arg1	incubated					728:736	incubated	728:736	was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size	724:814	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	7	19	theme	crude	1363:1367	arg1	CP					1378:1379	CP	1378:1379	CP	1378:1379	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	7	19	theme	crude	1363:1367	arg1	protein					1369:1375	crude protein	1363:1375	crude protein (CP)	1363:1380	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	9	20	theme	SSB	1643:1645	arg1	level					1647:1651	an increase SSB level	1631:1651	an increase SSB level in TMR	1631:1658	As an increase SSB level in TMR, there was a decrease in acetate to propionate ratio at 8, 16, 24, and 48 h (p < 0.05).					
35223213	0	21	theme	dairy	138:142	arg1	cow					144:146	the lactating dairy cow	124:146	the lactating dairy cow	124:146	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	7	22	theme	DM	1356:1357	arg1	fraction					1331:1338	RESULTS Soluble fraction	1315:1338	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP)	1315:1380	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	7	22	theme	DM	1356:1357	arg1	a					1350:1350	fraction a	1341:1350	fraction a	1341:1350	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	2	23	theme	fluid	433:437	arg1	mixture					439:445	the buffer-rumen fluid mixture	416:445	the buffer-rumen fluid mixture	416:445	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	1	24	theme	in-vitro	275:282	arg1	digestibility					284:296	the rumen in-vitro digestibility	265:296	the rumen in-vitro digestibility	265:296	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	9	25	from	decrease	1673:1680	arg1	ratio					1707:1711	acetate to propionate ratio	1685:1711	acetate to propionate ratio	1685:1711	As an increase SSB level in TMR, there was a decrease in acetate to propionate ratio at 8, 16, 24, and 48 h (p < 0.05).					
35223213	9	26	from	level	1647:1651	arg1	TMR					1656:1658	TMR	1656:1658	TMR	1656:1658	As an increase SSB level in TMR, there was a decrease in acetate to propionate ratio at 8, 16, 24, and 48 h (p < 0.05).					
35223213	7	27	theme	Soluble	1323:1329	arg1	fraction					1331:1338	RESULTS Soluble fraction	1315:1338	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP)	1315:1380	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	7	27	theme	Soluble	1323:1329	arg1	a					1350:1350	fraction a	1341:1350	fraction a	1341:1350	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	7	28	theme	fraction	1341:1348	arg1	fraction					1331:1338	RESULTS Soluble fraction	1315:1338	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP)	1315:1380	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	7	28	theme	fraction	1341:1348	arg1	a					1350:1350	fraction a	1341:1350	fraction a	1341:1350	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	0	29	from	Effects	0:6	arg1	productivity					108:119	milking productivity	100:119	milking productivity of the lactating dairy cow	100:146	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	0	29	from	Effects	0:6	arg1	rate					90:93	in situ disappearance rate	68:93	in situ disappearance rate	68:93	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	0	29	from	Effects	0:6	arg1	digestibility					53:65	rumen in vitro digestibility	38:65	rumen in vitro digestibility	38:65	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	5	30	theme	national	1139:1146	arg1	NRC					1166:1168	NRC	1166:1168	NRC	1166:1168	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	5	30	theme	national	1139:1146	arg1	council					1157:1163	national research council	1139:1163	national research council (NRC) nutrient requirements of dairy cattle guideline	1139:1217	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	4	31	theme	Friesian	909:916	arg1	cows					918:921	sixteen Holstein Friesian cows	892:921	sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght)	892:982	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	7	32	theme	CP	1477:1478	arg1	value					1461:1465	c value	1459:1465	c value of DM and CP	1459:1478	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	10	33	theme	matter	1752:1757	arg1	intake					1759:1764	Dry matter intake	1748:1764	Dry matter intake	1748:1764	Dry matter intake, milk production, and milk composition did not differ between the treatments.					
35223213	11	34	theme	profile	1854:1860	arg1	contents					1862:1869	All blood profile contents	1844:1869	All blood profile contents	1844:1869	All blood profile contents did not differ between treatments.					
35223213	8	35	theme	mixed	1538:1542	arg1	ration					1544:1549	total mixed ration	1532:1549	total mixed ration (TMR)	1532:1555	As an increase SSB level in total mixed ration (TMR), there was a decrease in gas production at 0, 16, and 48 h (p < 0.05).					
35223213	8	35	theme	mixed	1538:1542	arg1	TMR					1552:1554	TMR	1552:1554	TMR	1552:1554	As an increase SSB level in total mixed ration (TMR), there was a decrease in gas production at 0, 16, and 48 h (p < 0.05).					
35223213	5	36	with	diet	1066:1069	arg1	diet					1110:1113	the diet	1106:1113	the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline	1106:1217	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	3	37	theme	*	803:803	arg1	size					811:814	45*m pore size	801:814	45*m pore size	801:814	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	3	37	theme	*	803:803	arg1	hours					769:773	0, 2, 4, 8, 16, 24, and 48 hours	742:773	0, 2, 4, 8, 16, 24, and 48 hours in nylon bags	742:787	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	12	38	dep	CONCLUSION	1906:1915	arg1	fed					1953:1955	fed	1953:1955	could be fed to ruminants without adverse effects on rumen fermentation	1944:2014	CONCLUSION A diet containing 3.0% SSB could be fed to ruminants without adverse effects on rumen fermentation.					
35223213	3	39	theme	in-situ	692:698	arg1	rate					714:717	in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size)	692:815	the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows	688:877	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	7	40	dep	%	1449:1449	arg1	DM					1451:1452	DM	1451:1452	44.63 and 31.61% DM	1434:1452	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	9	41	theme	acetate	1685:1691	arg1	ratio					1707:1711	acetate to propionate ratio	1685:1711	acetate to propionate ratio	1685:1711	As an increase SSB level in TMR, there was a decrease in acetate to propionate ratio at 8, 16, 24, and 48 h (p < 0.05).					
35223213	8	42	theme	SSB	1519:1521	arg1	level					1523:1527	an increase SSB level	1507:1527	an increase SSB level in total mixed ration (TMR)	1507:1555	As an increase SSB level in total mixed ration (TMR), there was a decrease in gas production at 0, 16, and 48 h (p < 0.05).					
35223213	1	43	theme	by-product	237:246	arg1	feeding					254:260	starch sugar by-product (SSB) feeding	224:260	starch sugar by-product (SSB) feeding	224:260	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	2	44	dep	in	383:384	arg1	vitro					386:390	vitro	386:390	vitro	386:390	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	6	45	theme	randomized	1255:1264	arg1	design					1272:1277	a randomized block design	1253:1277	a randomized block design for six weeks based on body weight	1253:1312	An experiment was conducted with a randomized block design for six weeks based on body weight.					
35223213	2	46	theme	rumen	377:381	arg1	digestibility					392:404	the rumen in vitro digestibility	373:404	the rumen in vitro digestibility	373:404	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	1	47	dep	in	299:300	arg1	situ					302:305	situ	302:305	situ	302:305	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	5	48	theme	nutrient	1171:1178	arg1	requirements					1180:1191	national research council (NRC) nutrient requirements	1139:1191	national research council (NRC) nutrient requirements of dairy cattle guideline	1139:1217	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	2	49	contain	containing	488:497	arg2	g					503:503	0.5 g	499:503	0.5 g of dry matter (DM) of substrates	499:536	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	2	49	contain	containing	488:497	arg1	serum					475:479	a 125 mL serum bottle	466:486	a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates	466:536	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	9	50	theme	propionate	1696:1705	arg1	ratio					1707:1711	acetate to propionate ratio	1685:1711	acetate to propionate ratio	1685:1711	As an increase SSB level in TMR, there was a decrease in acetate to propionate ratio at 8, 16, 24, and 48 h (p < 0.05).					
35223213	0	51	theme	starch	11:16	arg1	by-product					24:33	starch sugar by-product	11:33	starch sugar by-product	11:33	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	1	52	theme	SSB	249:251	arg1	feeding					254:260	starch sugar by-product (SSB) feeding	224:260	starch sugar by-product (SSB) feeding	224:260	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	4	53	theme	kg	960:961	arg1	months					936:941	60.5 ± 20.4 months	924:941	60.5 ± 20.4 months old	924:945	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	4	53	theme	kg	960:961	arg1	wieght					976:981	706.8 ± 3.4 kg initial body wieght	948:981	706.8 ± 3.4 kg initial body wieght	948:981	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	0	54	theme	by-product	24:33	arg1	Effects					0:6	Effects	0:6	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.	0:147	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	1	55	theme	feeding	254:260	arg1	effects					213:219	the effects	209:219	the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow	209:349	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	3	56	theme	pore	806:809	arg1	size					811:814	45*m pore size	801:814	45*m pore size	801:814	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	3	56	theme	pore	806:809	arg1	hours					769:773	0, 2, 4, 8, 16, 24, and 48 hours	742:773	0, 2, 4, 8, 16, 24, and 48 hours in nylon bags	742:787	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	5	57	theme	council	1157:1163	arg1	requirements					1180:1191	national research council (NRC) nutrient requirements	1139:1191	national research council (NRC) nutrient requirements of dairy cattle guideline	1139:1217	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	4	58	theme	body	971:974	arg1	months					936:941	60.5 ± 20.4 months	924:941	60.5 ± 20.4 months old	924:945	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	4	58	theme	body	971:974	arg1	wieght					976:981	706.8 ± 3.4 kg initial body wieght	948:981	706.8 ± 3.4 kg initial body wieght	948:981	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	0	59	theme	rumen	38:42	arg1	digestibility					53:65	rumen in vitro digestibility	38:65	rumen in vitro digestibility	38:65	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	5	60	with	DM	1090:1091	arg1	diet					1110:1113	the diet	1106:1113	the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline	1106:1217	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	3	61	theme	ventral	835:841	arg1	sac					843:845	the ventral sac	831:845	the ventral sac of two cannulated Holstein cows	831:877	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	7	62	dep	28.99	1386:1390	arg1	DM					1402:1403	DM	1402:1403	DM	1402:1403	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	7	63	theme	fraction	1406:1413	arg1	b					1415:1415	fraction b	1406:1415	fraction b of DM and CP	1406:1428	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	3	64	theme	cannulated	854:863	arg1	cows					874:877	two cannulated Holstein cows	850:877	two cannulated Holstein cows	850:877	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	8	65	from	decrease	1570:1577	arg1	production					1586:1595	gas production	1582:1595	gas production	1582:1595	As an increase SSB level in total mixed ration (TMR), there was a decrease in gas production at 0, 16, and 48 h (p < 0.05).					
35223213	3	66	dep	N2	553:554	arg1	%					561:561	99.9% pure	557:566	99.9% pure	557:566	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	2	67	theme	matter	512:517	arg1	g					503:503	0.5 g	499:503	0.5 g of dry matter (DM) of substrates	499:536	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	3	68	theme	Nitrogen	539:546	arg1	gas					548:550	Nitrogen gas	539:550	Nitrogen gas (N2, 99.9% pure)	539:567	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	3	68	theme	Nitrogen	539:546	arg1	N2					553:554	N2	553:554	N2	553:554	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	5	69	theme	cattle	1202:1207	arg1	guideline					1209:1217	dairy cattle guideline	1196:1217	dairy cattle guideline	1196:1217	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	4	70	theme	experimental	1018:1029	arg1	periods					1031:1037	the experimental periods	1014:1037	the experimental periods	1014:1037	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	1	71	theme	starch	224:229	arg1	feeding					254:260	starch sugar by-product (SSB) feeding	224:260	starch sugar by-product (SSB) feeding	224:260	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	3	72	theme	cows	874:877	arg1	sac					843:845	the ventral sac	831:845	the ventral sac of two cannulated Holstein cows	831:877	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	4	73	theme	experimental	988:999	arg1	diets					1001:1005	experimental diets	988:1005	experimental diets	988:1005	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	0	74	theme	milking	100:106	arg1	productivity					108:119	milking productivity	100:119	milking productivity of the lactating dairy cow	100:146	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	5	75	theme	%	1088:1088	arg1	DM					1090:1091	3.0% DM	1085:1091	3.0% DM of SSB	1085:1098	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	2	76	theme	mL	472:473	arg1	serum					475:479	a 125 mL serum bottle	466:486	a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates	466:536	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	1	77	from	effects	213:219	arg1	cow					347:349	lactating dairy cow	331:349	lactating dairy cow	331:349	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	1	77	from	effects	213:219	arg1	rate					321:324	in situ disappearance rate	299:324	in situ disappearance rate	299:324	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	1	77	from	effects	213:219	arg1	digestibility					284:296	the rumen in-vitro digestibility	265:296	the rumen in-vitro digestibility	265:296	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	1	78	theme	lactating	331:339	arg1	cow					347:349	lactating dairy cow	331:349	lactating dairy cow	331:349	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	1	79	theme	present	178:184	arg1	study					186:190	the present study	174:190	the present study	174:190	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	0	80	theme	lactating	128:136	arg1	cow					144:146	the lactating dairy cow	124:146	the lactating dairy cow	124:146	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	12	81	theme	rumen	1997:2001	arg1	fermentation					2003:2014	rumen fermentation	1997:2014	rumen fermentation	1997:2014	CONCLUSION A diet containing 3.0% SSB could be fed to ruminants without adverse effects on rumen fermentation.					
35223213	9	82	theme	increase	1634:1641	arg1	level					1647:1651	an increase SSB level	1631:1651	an increase SSB level in TMR	1631:1658	As an increase SSB level in TMR, there was a decrease in acetate to propionate ratio at 8, 16, 24, and 48 h (p < 0.05).					
35223213	0	83	theme	cow	144:146	arg1	productivity					108:119	milking productivity	100:119	milking productivity of the lactating dairy cow	100:146	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	0	83	theme	cow	144:146	arg1	rate					90:93	in situ disappearance rate	68:93	in situ disappearance rate	68:93	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	0	83	theme	cow	144:146	arg1	digestibility					53:65	rumen in vitro digestibility	38:65	rumen in vitro digestibility	38:65	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	12	84	theme	%	1938:1938	arg1	SSB					1940:1942	3.0% SSB	1935:1942	3.0% SSB	1935:1942	CONCLUSION A diet containing 3.0% SSB could be fed to ruminants without adverse effects on rumen fermentation.					
35223213	12	85	from	effects	1986:1992	arg1	fermentation					2003:2014	rumen fermentation	1997:2014	rumen fermentation	1997:2014	CONCLUSION A diet containing 3.0% SSB could be fed to ruminants without adverse effects on rumen fermentation.					
35223213	7	86	theme	protein	1369:1375	arg1	fraction					1331:1338	RESULTS Soluble fraction	1315:1338	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP)	1315:1380	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	7	86	theme	protein	1369:1375	arg1	a					1350:1350	fraction a	1341:1350	fraction a	1341:1350	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	2	87	theme	bottle	481:486	arg1	serum					475:479	a 125 mL serum bottle	466:486	a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates	466:536	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	6	88	theme	body	1302:1305	arg1	weight					1307:1312	body weight	1302:1312	body weight	1302:1312	An experiment was conducted with a randomized block design for six weeks based on body weight.					
35223213	2	89	theme	buffer-rumen	420:431	arg1	mixture					439:445	the buffer-rumen fluid mixture	416:445	the buffer-rumen fluid mixture	416:445	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	9	90	dep	ratio	1707:1711	arg1	to					1693:1694	to	1693:1694	to	1693:1694	As an increase SSB level in TMR, there was a decrease in acetate to propionate ratio at 8, 16, 24, and 48 h (p < 0.05).					
35223213	0	91	theme	in	68:69	arg1	rate					90:93	in situ disappearance rate	68:93	in situ disappearance rate	68:93	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	8	92	from	level	1523:1527	arg1	ration					1544:1549	total mixed ration	1532:1549	total mixed ration (TMR)	1532:1555	As an increase SSB level in total mixed ration (TMR), there was a decrease in gas production at 0, 16, and 48 h (p < 0.05).					
35223213	8	92	from	level	1523:1527	arg1	TMR					1552:1554	TMR	1552:1554	TMR	1552:1554	As an increase SSB level in total mixed ration (TMR), there was a decrease in gas production at 0, 16, and 48 h (p < 0.05).					
35223213	4	93	theme	cows	918:921	arg1	total					883:887	A total	881:887	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght)	881:982	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	0	94	theme	disappearance	76:88	arg1	rate					90:93	in situ disappearance rate	68:93	in situ disappearance rate	68:93	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	1	95	theme	in	299:300	arg1	rate					321:324	in situ disappearance rate	299:324	in situ disappearance rate	299:324	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	3	96	theme	nylon	778:782	arg1	bags					784:787	nylon bags	778:787	nylon bags	778:787	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	11	97	theme	blood	1848:1852	arg1	contents					1862:1869	All blood profile contents	1844:1869	All blood profile contents	1844:1869	All blood profile contents did not differ between treatments.					
35223213	5	98	theme	research	1148:1155	arg1	NRC					1166:1168	NRC	1166:1168	NRC	1166:1168	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	5	98	theme	research	1148:1155	arg1	council					1157:1163	national research council	1139:1163	national research council (NRC) nutrient requirements of dairy cattle guideline	1139:1217	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	8	99	theme	increase	1510:1517	arg1	level					1523:1527	an increase SSB level	1507:1527	an increase SSB level in total mixed ration (TMR)	1507:1555	As an increase SSB level in total mixed ration (TMR), there was a decrease in gas production at 0, 16, and 48 h (p < 0.05).					
35223213	1	100	theme	disappearance	307:319	arg1	rate					321:324	in situ disappearance rate	299:324	in situ disappearance rate	299:324	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	7	101	theme	DM	1470:1471	arg1	value					1461:1465	c value	1459:1465	c value of DM and CP	1459:1478	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	2	102	theme	mixture	439:445	arg1	mL					410:411	50 mL	407:411	50 mL of the buffer-rumen fluid mixture	407:445	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	7	103	theme	c	1459:1459	arg1	value					1461:1465	c value	1459:1465	c value of DM and CP	1459:1478	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	6	104	theme	block	1266:1270	arg1	design					1272:1277	a randomized block design	1253:1277	a randomized block design for six weeks based on body weight	1253:1312	An experiment was conducted with a randomized block design for six weeks based on body weight.					
35223213	0	105	dep	in	68:69	arg1	situ					71:74	situ	71:74	situ	71:74	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	3	106	theme	disappearance	700:712	arg1	rate					714:717	in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size)	692:815	the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows	688:877	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	2	107	theme	in	383:384	arg1	digestibility					392:404	the rumen in vitro digestibility	373:404	the rumen in vitro digestibility	373:404	METHODS To determine the rumen in vitro digestibility, 50 mL of the buffer-rumen fluid mixture was dispensed into a 125 mL serum bottle containing 0.5 g of dry matter (DM) of substrates.					
35223213	4	108	theme	Holstein	900:907	arg1	cows					918:921	sixteen Holstein Friesian cows	892:921	sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght)	892:982	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	0	109	theme	sugar	18:22	arg1	by-product					24:33	starch sugar by-product	11:33	starch sugar by-product	11:33	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	10	110	theme	milk	1767:1770	arg1	production					1772:1781	milk production	1767:1781	milk production	1767:1781	Dry matter intake, milk production, and milk composition did not differ between the treatments.					
35223213	4	111	theme	initial	963:969	arg1	months					936:941	60.5 ± 20.4 months	924:941	60.5 ± 20.4 months old	924:945	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	4	111	theme	initial	963:969	arg1	wieght					976:981	706.8 ± 3.4 kg initial body wieght	948:981	706.8 ± 3.4 kg initial body wieght	948:981	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	3	112	theme	m	804:804	arg1	size					811:814	45*m pore size	801:814	45*m pore size	801:814	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	3	112	theme	m	804:804	arg1	hours					769:773	0, 2, 4, 8, 16, 24, and 48 hours	742:773	0, 2, 4, 8, 16, 24, and 48 hours in nylon bags	742:787	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	0	113	dep	in	44:45	arg1	vitro					47:51	vitro	47:51	vitro	47:51	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	8	114	theme	gas	1582:1584	arg1	production					1586:1595	gas production	1582:1595	gas production	1582:1595	As an increase SSB level in total mixed ration (TMR), there was a decrease in gas production at 0, 16, and 48 h (p < 0.05).					
35223213	5	115	theme	dairy	1196:1200	arg1	guideline					1209:1217	dairy cattle guideline	1196:1217	dairy cattle guideline	1196:1217	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	5	116	theme	basal	1060:1064	arg1	treatments					1044:1053	The treatments	1040:1053	The treatments	1040:1053	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	5	116	theme	basal	1060:1064	arg1	diet					1066:1069	basal diet	1060:1069	basal diet (control)	1060:1079	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	5	116	theme	basal	1060:1064	arg1	control					1072:1078	control	1072:1078	control	1072:1078	The treatments were basal diet (control) and 3.0% DM of SSB, with the diet formulated according to national research council (NRC) nutrient requirements of dairy cattle guideline.					
35223213	10	117	theme	Dry	1748:1750	arg1	intake					1759:1764	Dry matter intake	1748:1764	Dry matter intake	1748:1764	Dry matter intake, milk production, and milk composition did not differ between the treatments.					
35223213	0	118	theme	in	44:45	arg1	digestibility					53:65	rumen in vitro digestibility	38:65	rumen in vitro digestibility	38:65	Effects of starch sugar by-product on rumen in vitro digestibility, in situ disappearance rate, and milking productivity of the lactating dairy cow.					
35223213	7	119	theme	CP	1427:1428	arg1	b					1415:1415	fraction b	1406:1415	fraction b of DM and CP	1406:1428	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	10	120	theme	milk	1788:1791	arg1	composition					1793:1803	milk composition	1788:1803	milk composition	1788:1803	Dry matter intake, milk production, and milk composition did not differ between the treatments.					
35223213	4	121	theme	old	943:945	arg1	months					936:941	60.5 ± 20.4 months	924:941	60.5 ± 20.4 months old	924:945	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	4	121	theme	old	943:945	arg1	wieght					976:981	706.8 ± 3.4 kg initial body wieght	948:981	706.8 ± 3.4 kg initial body wieght	948:981	A total of sixteen Holstein Friesian cows (60.5 ± 20.4 months old, 706.8 ± 3.4 kg initial body wieght) fed experimental diets during the experimental periods.					
35223213	1	122	dep	OBJECTIVE	149:157	arg1	was					192:194	was	192:194	was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow	192:349	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	1	123	theme	rumen	269:273	arg1	digestibility					284:296	the rumen in-vitro digestibility	265:296	the rumen in-vitro digestibility	265:296	OBJECTIVE The purpose of the present study was to determine the effects of starch sugar by-product (SSB) feeding on the rumen in-vitro digestibility, in situ disappearance rate, and lactating dairy cow.					
35223213	7	124	theme	DM	1420:1421	arg1	b					1415:1415	fraction b	1406:1415	fraction b of DM and CP	1406:1428	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	3	125	from	hours	769:773	arg1	bags					784:787	nylon bags	778:787	nylon bags	778:787	Nitrogen gas (N2, 99.9% pure) was flushed into the serum bottles and three replications were incubated at 0, 2, 4, 8, 16, 24, and 48 h. To determine the in-situ disappearance rate, SSB was incubated for 0, 2, 4, 8, 16, 24, and 48 hours in nylon bags (5 × 10 cm, 45*m pore size) placed within the ventral sac of two cannulated Holstein cows.					
35223213	12	126	contain	containing	1924:1933	arg2	SSB					1940:1942	3.0% SSB	1935:1942	3.0% SSB	1935:1942	CONCLUSION A diet containing 3.0% SSB could be fed to ruminants without adverse effects on rumen fermentation.					
35223213	12	126	contain	containing	1924:1933	arg1	diet					1919:1922	A diet	1917:1922	A diet containing 3.0% SSB	1917:1942	CONCLUSION A diet containing 3.0% SSB could be fed to ruminants without adverse effects on rumen fermentation.					
35223213	7	127	theme	RESULTS	1315:1321	arg1	fraction					1331:1338	RESULTS Soluble fraction	1315:1338	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP)	1315:1380	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35223213	7	127	theme	RESULTS	1315:1321	arg1	a					1350:1350	fraction a	1341:1350	fraction a	1341:1350	RESULTS Soluble fraction (fraction a) of DM and crude protein (CP) was 28.99 and 11.92%DM, fraction b of DM and CP was 44.63 and 31.61% DM, and c value of DM and CP was 26.38 and 56.47%DM.					
35714870	7	0	theme	cells	1114:1118	arg1	assays					1057:1062	The biological assays both MTT assay and cell attachment	1042:1097	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers	1042:1168	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	7	0	theme	cells	1114:1118	arg1	assay					1073:1077	MTT assay	1069:1077	MTT assay	1069:1077	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	7	0	theme	cells	1114:1118	arg1	attachment					1088:1097	cell attachment	1083:1097	cell attachment	1083:1097	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	3	1	theme	long-term	576:584	arg1	applications					586:597	long-term applications	576:597	long-term applications like cartilage regeneration	576:625	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration.					
35714870	7	2	theme	biomedical	1268:1277	arg1	applications					1279:1290	biomedical applications	1268:1290	biomedical applications	1268:1290	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	3	3	theme	hydrophobic	363:373	arg1	polyhydroxybutyrate					375:393	hydrophobic polyhydroxybutyrate	363:393	hydrophobic polyhydroxybutyrate (PHB)	363:399	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration.					
35714870	3	3	theme	hydrophobic	363:373	arg1	PHB					396:398	PHB	396:398	PHB	396:398	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration.					
35714870	1	4	theme	regeneration	182:193	arg1	rate					174:177	a low rate	168:177	a low rate of regeneration due to slow metabolism	168:216	Articular cartilage is a connective load-bearing tissue with a low rate of regeneration due to slow metabolism.					
35714870	5	5	dep	incorporation	906:918	arg1	support					920:926	support	920:926	support chondrocyte cell growth	920:950	Moreover, the tensile strength was improved up to 4.21 ± 0.31 MPa after HNTs incorporation support chondrocyte cell growth.					
35714870	3	6	theme	halloysite	441:450	arg1	HNTs					463:466	HNTs	463:466	HNTs	463:466	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration.					
35714870	3	6	theme	halloysite	441:450	arg1	nanotubes					452:460	halloysite nanotubes	441:460	halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties	441:527	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration.					
35714870	3	7	with	nanotubes	452:460	arg1	physico-chemical					486:501	physico-chemical	486:501	physico-chemical	486:501	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration.					
35714870	3	7	with	nanotubes	452:460	arg1	properties					518:527	appropriate physico-chemical and biological properties	474:527	properties	518:527	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration.					
35714870	4	8	theme	incorporating	694:706	arg1	HNTs					708:711	incorporating HNTs	694:711	incorporating HNTs up to 2 wt%	694:723	Textural properties were analyzed through SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage.					
35714870	8	9	theme	obtained	1310:1317	arg1	results					1319:1325	the obtained results	1306:1325	the obtained results	1306:1325	To conclude, the obtained results demonstrated PHB-starch/HNTs fibrous scaffold could be potential for further experimental studies for tissue engineering applications like cartilage.					
35714870	4	10	theme	porosity	808:815	arg1	percentage					817:826	appropriate porosity percentage	796:826	appropriate porosity percentage	796:826	Textural properties were analyzed through SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage.					
35714870	7	11	theme	cell	1083:1086	arg1	assays					1057:1062	The biological assays both MTT assay and cell attachment	1042:1097	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers	1042:1168	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	7	11	theme	cell	1083:1086	arg1	attachment					1088:1097	cell attachment	1083:1097	cell attachment	1083:1097	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	8	12	theme	further	1396:1402	arg1	studies					1417:1423	further experimental studies	1396:1423	further experimental studies	1396:1423	To conclude, the obtained results demonstrated PHB-starch/HNTs fibrous scaffold could be potential for further experimental studies for tissue engineering applications like cartilage.					
35714870	5	13	theme	tensile	843:849	arg1	strength					851:858	the tensile strength	839:858	the tensile strength	839:858	Moreover, the tensile strength was improved up to 4.21 ± 0.31 MPa after HNTs incorporation support chondrocyte cell growth.					
35714870	7	14	theme	MTT	1069:1071	arg1	assays					1057:1062	The biological assays both MTT assay and cell attachment	1042:1097	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers	1042:1168	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	7	14	theme	MTT	1069:1071	arg1	assay					1073:1077	MTT assay	1069:1077	MTT assay	1069:1077	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	1	15	theme	Articular	107:115	arg1	tissue					156:161	a connective load-bearing tissue	130:161	a connective load-bearing tissue with a low rate of regeneration due to slow metabolism	130:216	Articular cartilage is a connective load-bearing tissue with a low rate of regeneration due to slow metabolism.					
35714870	1	15	theme	Articular	107:115	arg1	cartilage					117:125	Articular cartilage	107:125	Articular cartilage	107:125	Articular cartilage is a connective load-bearing tissue with a low rate of regeneration due to slow metabolism.					
35714870	0	16	theme	halloysite	12:21	arg1	nanotube					23:30	Electrospun halloysite nanotube	0:30	Electrospun halloysite nanotube	0:30	Electrospun halloysite nanotube loaded polyhydroxybutyrate-starch fibers for cartilage tissue engineering.					
35714870	7	17	theme	cell	1216:1219	arg1	growth					1221:1226	cell growth	1216:1226	cell growth	1216:1226	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	6	18	theme	in	1020:1021	arg1	degradation					1029:1039	in vitro degradation	1020:1039	in vitro degradation	1020:1039	Furthermore, incorporating HNTs induced surface hydrophilicity and in vitro degradation.					
35714870	1	19	theme	slow	202:205	arg1	metabolism					207:216	slow metabolism	202:216	slow metabolism	202:216	Articular cartilage is a connective load-bearing tissue with a low rate of regeneration due to slow metabolism.					
35714870	0	20	theme	Electrospun	0:10	arg1	nanotube					23:30	Electrospun halloysite nanotube	0:30	Electrospun halloysite nanotube	0:30	Electrospun halloysite nanotube loaded polyhydroxybutyrate-starch fibers for cartilage tissue engineering.					
35714870	1	21	with	tissue	156:161	arg1	rate					174:177	a low rate	168:177	a low rate of regeneration due to slow metabolism	168:216	Articular cartilage is a connective load-bearing tissue with a low rate of regeneration due to slow metabolism.					
35714870	4	22	dep	showed	687:692	arg1	decreased					725:733	decreased	725:733	showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage	687:826	Textural properties were analyzed through SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage.					
35714870	3	23	theme	starch	417:422	arg1	composition					348:358	the composition	344:358	the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties	344:527	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration.					
35714870	3	24	theme	Fibrous	317:323	arg1	scaffolds					325:333	Fibrous scaffolds	317:333	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties	317:527	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration.					
35714870	8	25	theme	fibrous	1356:1362	arg1	scaffold					1364:1371	PHB-starch/HNTs fibrous scaffold	1340:1371	PHB-starch/HNTs fibrous scaffold	1340:1371	To conclude, the obtained results demonstrated PHB-starch/HNTs fibrous scaffold could be potential for further experimental studies for tissue engineering applications like cartilage.					
35714870	8	26	theme	tissue	1429:1434	arg1	applications					1448:1459	tissue engineering applications	1429:1459	tissue engineering applications like cartilage	1429:1474	To conclude, the obtained results demonstrated PHB-starch/HNTs fibrous scaffold could be potential for further experimental studies for tissue engineering applications like cartilage.					
35714870	5	27	theme	cell	940:943	arg1	growth					945:950	chondrocyte cell growth	928:950	chondrocyte cell growth	928:950	Moreover, the tensile strength was improved up to 4.21 ± 0.31 MPa after HNTs incorporation support chondrocyte cell growth.					
35714870	4	28	theme	SEM	670:672	arg1	imaging					674:680	SEM imaging	670:680	SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage	670:826	Textural properties were analyzed through SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage.					
35714870	6	29	theme	surface	993:999	arg1	hydrophilicity					1001:1014	surface hydrophilicity	993:1014	surface hydrophilicity	993:1014	Furthermore, incorporating HNTs induced surface hydrophilicity and in vitro degradation.					
35714870	1	30	theme	connective	132:141	arg1	tissue					156:161	a connective load-bearing tissue	130:161	a connective load-bearing tissue with a low rate of regeneration due to slow metabolism	130:216	Articular cartilage is a connective load-bearing tissue with a low rate of regeneration due to slow metabolism.					
35714870	1	30	theme	connective	132:141	arg1	cartilage					117:125	Articular cartilage	107:125	Articular cartilage	107:125	Articular cartilage is a connective load-bearing tissue with a low rate of regeneration due to slow metabolism.					
35714870	4	31	theme	pore	782:785	arg1	size					787:790	larger pore size	775:790	larger pore size	775:790	Textural properties were analyzed through SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage.					
35714870	7	32	theme	HNTs	1185:1188	arg1	incorporation					1190:1202	HNTs incorporation	1185:1202	HNTs incorporation	1185:1202	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	4	33	theme	fiber	740:744	arg1	diameter					746:753	mean fiber diameter	735:753	mean fiber diameter to 158 ± 48 nm	735:768	Textural properties were analyzed through SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage.					
35714870	2	34	theme	natural	271:277	arg1	features					279:286	natural features	271:286	natural features	271:286	Fabricating tissue-like structure modified based on natural features can improve healing process.					
35714870	8	35	theme	experimental	1404:1415	arg1	studies					1417:1423	further experimental studies	1396:1423	further experimental studies	1396:1423	To conclude, the obtained results demonstrated PHB-starch/HNTs fibrous scaffold could be potential for further experimental studies for tissue engineering applications like cartilage.					
35714870	3	36	theme	biological	507:516	arg1	properties					518:527	appropriate physico-chemical and biological properties	474:527	properties	518:527	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration.					
35714870	0	37	theme	polyhydroxybutyrate-starch	39:64	arg1	fibers					66:71	polyhydroxybutyrate-starch fibers	39:71	polyhydroxybutyrate-starch fibers	39:71	Electrospun halloysite nanotube loaded polyhydroxybutyrate-starch fibers for cartilage tissue engineering.					
35714870	5	38	theme	chondrocyte	928:938	arg1	growth					945:950	chondrocyte cell growth	928:950	chondrocyte cell growth	928:950	Moreover, the tensile strength was improved up to 4.21 ± 0.31 MPa after HNTs incorporation support chondrocyte cell growth.					
35714870	7	39	from	assays	1057:1062	arg1	HNTs					1129:1132	2 wt% HNTs	1123:1132	2 wt% HNTs incorporated into PHB-starch fibers	1123:1168	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	4	40	theme	mean	735:738	arg1	diameter					746:753	mean fiber diameter	735:753	mean fiber diameter to 158 ± 48 nm	735:768	Textural properties were analyzed through SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage.					
35714870	0	41	theme	cartilage	77:85	arg1	engineering					94:104	cartilage tissue engineering	77:104	cartilage tissue engineering	77:104	Electrospun halloysite nanotube loaded polyhydroxybutyrate-starch fibers for cartilage tissue engineering.					
35714870	6	42	theme	incorporating	966:978	arg1	HNTs					980:983	incorporating HNTs	966:983	incorporating HNTs	966:983	Furthermore, incorporating HNTs induced surface hydrophilicity and in vitro degradation.					
35714870	1	43	theme	load-bearing	143:154	arg1	tissue					156:161	a connective load-bearing tissue	130:161	a connective load-bearing tissue with a low rate of regeneration due to slow metabolism	130:216	Articular cartilage is a connective load-bearing tissue with a low rate of regeneration due to slow metabolism.					
35714870	1	43	theme	load-bearing	143:154	arg1	cartilage					117:125	Articular cartilage	107:125	Articular cartilage	107:125	Articular cartilage is a connective load-bearing tissue with a low rate of regeneration due to slow metabolism.					
35714870	5	44	theme	HNTs	901:904	arg1	incorporation					906:918	HNTs incorporation	901:918	HNTs incorporation support chondrocyte cell growth	901:950	Moreover, the tensile strength was improved up to 4.21 ± 0.31 MPa after HNTs incorporation support chondrocyte cell growth.					
35714870	4	45	dep	%	723:723	arg1	up					713:714	up	713:714	up	713:714	Textural properties were analyzed through SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage.					
35714870	7	46	theme	%	1127:1127	arg1	HNTs					1129:1132	2 wt% HNTs	1123:1132	2 wt% HNTs incorporated into PHB-starch fibers	1123:1168	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	4	47	theme	appropriate	796:806	arg1	percentage					817:826	appropriate porosity percentage	796:826	appropriate porosity percentage	796:826	Textural properties were analyzed through SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage.					
35714870	4	48	theme	Textural	628:635	arg1	properties					637:646	Textural properties	628:646	Textural properties	628:646	Textural properties were analyzed through SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage.					
35714870	7	49	theme	PHB-starch	1152:1161	arg1	fibers					1163:1168	PHB-starch fibers	1152:1168	PHB-starch fibers	1152:1168	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	2	50	theme	tissue-like	231:241	arg1	structure					243:251	Fabricating tissue-like structure	219:251	Fabricating tissue-like structure modified based on natural features	219:286	Fabricating tissue-like structure modified based on natural features can improve healing process.					
35714870	2	51	theme	healing	300:306	arg1	process					308:314	healing process	300:314	healing process	300:314	Fabricating tissue-like structure modified based on natural features can improve healing process.					
35714870	7	52	theme	biological	1046:1055	arg1	assays					1057:1062	The biological assays both MTT assay and cell attachment	1042:1097	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers	1042:1168	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	7	52	theme	biological	1046:1055	arg1	assay					1073:1077	MTT assay	1069:1077	MTT assay	1069:1077	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	7	52	theme	biological	1046:1055	arg1	attachment					1088:1097	cell attachment	1083:1097	cell attachment	1083:1097	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	2	53	theme	Fabricating	219:229	arg1	structure					243:251	Fabricating tissue-like structure	219:251	Fabricating tissue-like structure modified based on natural features	219:286	Fabricating tissue-like structure modified based on natural features can improve healing process.					
35714870	4	54	theme	larger	775:780	arg1	size					787:790	larger pore size	775:790	larger pore size	775:790	Textural properties were analyzed through SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage.					
35714870	3	55	theme	polyhydroxybutyrate	375:393	arg1	composition					348:358	the composition	344:358	the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties	344:527	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration.					
35714870	8	56	theme	engineering	1436:1446	arg1	applications					1448:1459	tissue engineering applications	1429:1459	tissue engineering applications like cartilage	1429:1474	To conclude, the obtained results demonstrated PHB-starch/HNTs fibrous scaffold could be potential for further experimental studies for tissue engineering applications like cartilage.					
35714870	7	57	theme	2 wt	1123:1126	arg1	HNTs					1129:1132	2 wt% HNTs	1123:1132	2 wt% HNTs incorporated into PHB-starch fibers	1123:1168	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	1	58	theme	low	170:172	arg1	rate					174:177	a low rate	168:177	a low rate of regeneration due to slow metabolism	168:216	Articular cartilage is a connective load-bearing tissue with a low rate of regeneration due to slow metabolism.					
35714870	0	59	theme	tissue	87:92	arg1	engineering					94:104	cartilage tissue engineering	77:104	cartilage tissue engineering	77:104	Electrospun halloysite nanotube loaded polyhydroxybutyrate-starch fibers for cartilage tissue engineering.					
35714870	8	60	theme	PHB-starch/HNTs	1340:1354	arg1	scaffold					1364:1371	PHB-starch/HNTs fibrous scaffold	1340:1371	PHB-starch/HNTs fibrous scaffold	1340:1371	To conclude, the obtained results demonstrated PHB-starch/HNTs fibrous scaffold could be potential for further experimental studies for tissue engineering applications like cartilage.					
35714870	7	61	theme	chondrocyte	1102:1112	arg1	cells					1114:1118	chondrocyte cells	1102:1118	chondrocyte cells	1102:1118	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	3	62	theme	cartilage	604:612	arg1	regeneration					614:625	cartilage regeneration	604:625	cartilage regeneration	604:625	Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration.					
35714870	7	63	dep	assays	1057:1062	arg1	assays					1057:1062	The biological assays both MTT assay and cell attachment	1042:1097	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers	1042:1168	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	7	63	dep	assays	1057:1062	arg1	assay					1073:1077	MTT assay	1069:1077	MTT assay	1069:1077	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	7	63	dep	assays	1057:1062	arg1	attachment					1088:1097	cell attachment	1083:1097	cell attachment	1083:1097	The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications.					
35714870	6	64	dep	in	1020:1021	arg1	vitro					1023:1027	vitro	1023:1027	vitro	1023:1027	Furthermore, incorporating HNTs induced surface hydrophilicity and in vitro degradation.					
36629825	0	0	theme	long-term	60:68	arg1	storage					70:76	long-term storage	60:76	long-term storage of human platelet concentrates	60:107	The use of pectins as part of a cryoprotective solution for long-term storage of human platelet concentrates.					
36629825	4	1	theme	basic	673:677	arg1	cryoprotectants					679:693	the basic cryoprotectants	669:693	the basic cryoprotectants	669:693	RESULT The study showed that of the basic cryoprotectants, the best effect in the preservation of PC was with dimethylacetamide (DMAC).					
36629825	7	2	theme	pectin	1122:1127	arg1	structure					1109:1117	the chemical structure	1096:1117	the chemical structure of pectin	1096:1127	Moreover, the chemical structure of pectin determines the level of its cryoprotective action in relation to the preservation of PC.					
36629825	7	3	from	level	1144:1148	arg1	relation					1182:1189	relation	1182:1189	relation to the preservation of PC	1182:1215	Moreover, the chemical structure of pectin determines the level of its cryoprotective action in relation to the preservation of PC.					
36629825	7	4	theme	chemical	1100:1107	arg1	structure					1109:1117	the chemical structure	1096:1117	the chemical structure of pectin	1096:1127	Moreover, the chemical structure of pectin determines the level of its cryoprotective action in relation to the preservation of PC.					
36629825	3	5	dep	MATERIALS	453:461	arg1	Samples					475:481	Samples	475:481	Samples	475:481	MATERIALS AND METHODS Samples of platelet concentrates (PC) were frozen under the protection of complex solutions and stored in an electric freezer at -80 degree C for 1 and 6 months.					
36629825	3	6	theme	complex	549:555	arg1	solutions					557:565	complex solutions	549:565	complex solutions	549:565	MATERIALS AND METHODS Samples of platelet concentrates (PC) were frozen under the protection of complex solutions and stored in an electric freezer at -80 degree C for 1 and 6 months.					
36629825	5	7	theme	low	900:902	arg1	C					929:929	-80 degree C	918:929	-80 degree C	918:929	The use of pectins as an additive to the base solution of DMAC statistically improves the preservation of PC after exposure to low temperatures (-80 degree C) for 30 and 180 days.					
36629825	5	7	theme	low	900:902	arg1	temperatures					904:915	low temperatures	900:915	low temperatures (-80 degree C)	900:930	The use of pectins as an additive to the base solution of DMAC statistically improves the preservation of PC after exposure to low temperatures (-80 degree C) for 30 and 180 days.					
36629825	7	8	theme	action	1172:1177	arg1	level					1144:1148	the level	1140:1148	the level of its cryoprotective action in relation to the preservation of PC	1140:1215	Moreover, the chemical structure of pectin determines the level of its cryoprotective action in relation to the preservation of PC.					
36629825	6	9	dep	CONCLUSIONS	953:963	arg1	conclude					968:975	conclude	968:975	conclude that DMAC is more promising as a basis for the development of a new combined cryoprotectant for PC freezing	968:1083	CONCLUSIONS We conclude that DMAC is more promising as a basis for the development of a new combined cryoprotectant for PC freezing.					
36629825	3	10	theme	solutions	557:565	arg1	protection					535:544	the protection	531:544	the protection of complex solutions	531:565	MATERIALS AND METHODS Samples of platelet concentrates (PC) were frozen under the protection of complex solutions and stored in an electric freezer at -80 degree C for 1 and 6 months.					
36629825	5	11	theme	PC	879:880	arg1	preservation					863:874	the preservation	859:874	the preservation of PC	859:880	The use of pectins as an additive to the base solution of DMAC statistically improves the preservation of PC after exposure to low temperatures (-80 degree C) for 30 and 180 days.					
36629825	0	12	theme	platelet	87:94	arg1	concentrates					96:107	human platelet concentrates	81:107	human platelet concentrates	81:107	The use of pectins as part of a cryoprotective solution for long-term storage of human platelet concentrates.					
36629825	3	13	theme	platelet	486:493	arg1	PC					509:510	PC	509:510	PC	509:510	MATERIALS AND METHODS Samples of platelet concentrates (PC) were frozen under the protection of complex solutions and stored in an electric freezer at -80 degree C for 1 and 6 months.					
36629825	3	13	theme	platelet	486:493	arg1	concentrates					495:506	platelet concentrates	486:506	platelet concentrates (PC)	486:511	MATERIALS AND METHODS Samples of platelet concentrates (PC) were frozen under the protection of complex solutions and stored in an electric freezer at -80 degree C for 1 and 6 months.					
36629825	1	14	theme	great	156:160	arg1	potential					162:170	great potential	156:170	great potential	156:170	BACKGROUND Pectins have unique properties and great potential to become an indispensable component of cryoprotective environment for platelet freezing.					
36629825	0	15	theme	human	81:85	arg1	concentrates					96:107	human platelet concentrates	81:107	human platelet concentrates	81:107	The use of pectins as part of a cryoprotective solution for long-term storage of human platelet concentrates.					
36629825	7	16	theme	PC	1214:1215	arg1	preservation					1198:1209	the preservation	1194:1209	the preservation of PC	1194:1215	Moreover, the chemical structure of pectin determines the level of its cryoprotective action in relation to the preservation of PC.					
36629825	5	17	theme	degree	922:927	arg1	C					929:929	-80 degree C	918:929	-80 degree C	918:929	The use of pectins as an additive to the base solution of DMAC statistically improves the preservation of PC after exposure to low temperatures (-80 degree C) for 30 and 180 days.					
36629825	5	17	theme	degree	922:927	arg1	temperatures					904:915	low temperatures	900:915	low temperatures (-80 degree C)	900:930	The use of pectins as an additive to the base solution of DMAC statistically improves the preservation of PC after exposure to low temperatures (-80 degree C) for 30 and 180 days.					
36629825	2	18	theme	cryoprotective	383:396	arg1	solution					398:405	a cryoprotective solution	381:405	a cryoprotective solution for platelets during low-temperature storage	381:450	OBJECTIVE To investigate the possibility of including pectins (apple pectin AU-701, tanacetan) into the composition of a cryoprotective solution for platelets during low-temperature storage.					
36629825	1	19	contain	have	129:132	arg2	properties					141:150	unique properties	134:150	unique properties	134:150	BACKGROUND Pectins have unique properties and great potential to become an indispensable component of cryoprotective environment for platelet freezing.					
36629825	1	19	contain	have	129:132	arg1	Pectins					121:127	BACKGROUND Pectins	110:127	BACKGROUND Pectins	110:127	BACKGROUND Pectins have unique properties and great potential to become an indispensable component of cryoprotective environment for platelet freezing.					
36629825	1	19	contain	have	129:132	arg2	potential					162:170	great potential	156:170	great potential	156:170	BACKGROUND Pectins have unique properties and great potential to become an indispensable component of cryoprotective environment for platelet freezing.					
36629825	2	20	theme	apple	325:329	arg1	tanacetan					346:354	tanacetan	346:354	tanacetan	346:354	OBJECTIVE To investigate the possibility of including pectins (apple pectin AU-701, tanacetan) into the composition of a cryoprotective solution for platelets during low-temperature storage.					
36629825	2	20	theme	apple	325:329	arg1	AU-701					338:343	apple pectin AU-701	325:343	apple pectin AU-701	325:343	OBJECTIVE To investigate the possibility of including pectins (apple pectin AU-701, tanacetan) into the composition of a cryoprotective solution for platelets during low-temperature storage.					
36629825	5	21	theme	base	814:817	arg1	solution					819:826	the base solution	810:826	the base solution of DMAC	810:834	The use of pectins as an additive to the base solution of DMAC statistically improves the preservation of PC after exposure to low temperatures (-80 degree C) for 30 and 180 days.					
36629825	6	22	theme	cryoprotectant	1054:1067	arg1	development					1024:1034	the development	1020:1034	the development of a new combined cryoprotectant for PC freezing	1020:1083	CONCLUSIONS We conclude that DMAC is more promising as a basis for the development of a new combined cryoprotectant for PC freezing.					
36629825	0	23	theme	pectins	11:17	arg1	use					4:6	The use	0:6	The use of pectins as part of a cryoprotective solution for long-term storage of human platelet concentrates.	0:108	The use of pectins as part of a cryoprotective solution for long-term storage of human platelet concentrates.					
36629825	4	24	from	effect	705:710	arg1	preservation					719:730	the preservation	715:730	the preservation of PC	715:736	RESULT The study showed that of the basic cryoprotectants, the best effect in the preservation of PC was with dimethylacetamide (DMAC).					
36629825	2	25	theme	pectins	316:322	arg1	possibility					291:301	the possibility	287:301	the possibility of including pectins (apple pectin AU-701, tanacetan)	287:355	OBJECTIVE To investigate the possibility of including pectins (apple pectin AU-701, tanacetan) into the composition of a cryoprotective solution for platelets during low-temperature storage.					
36629825	2	26	theme	low-temperature	428:442	arg1	storage					444:450	low-temperature storage	428:450	low-temperature storage	428:450	OBJECTIVE To investigate the possibility of including pectins (apple pectin AU-701, tanacetan) into the composition of a cryoprotective solution for platelets during low-temperature storage.					
36629825	0	27	theme	concentrates	96:107	arg1	storage					70:76	long-term storage	60:76	long-term storage of human platelet concentrates	60:107	The use of pectins as part of a cryoprotective solution for long-term storage of human platelet concentrates.					
36629825	2	28	theme	including	306:314	arg1	pectins					316:322	including pectins	306:322	including pectins (apple pectin AU-701, tanacetan)	306:355	OBJECTIVE To investigate the possibility of including pectins (apple pectin AU-701, tanacetan) into the composition of a cryoprotective solution for platelets during low-temperature storage.					
36629825	1	29	theme	indispensable	185:197	arg1	component					199:207	an indispensable component	182:207	an indispensable component of cryoprotective environment for platelet freezing	182:259	BACKGROUND Pectins have unique properties and great potential to become an indispensable component of cryoprotective environment for platelet freezing.					
36629825	2	30	theme	solution	398:405	arg1	composition					366:376	the composition	362:376	the composition of a cryoprotective solution for platelets during low-temperature storage	362:450	OBJECTIVE To investigate the possibility of including pectins (apple pectin AU-701, tanacetan) into the composition of a cryoprotective solution for platelets during low-temperature storage.					
36629825	3	31	theme	electric	584:591	arg1	freezer					593:599	an electric freezer	581:599	an electric freezer	581:599	MATERIALS AND METHODS Samples of platelet concentrates (PC) were frozen under the protection of complex solutions and stored in an electric freezer at -80 degree C for 1 and 6 months.					
36629825	2	32	dep	pectins	316:322	arg1	tanacetan					346:354	tanacetan	346:354	tanacetan	346:354	OBJECTIVE To investigate the possibility of including pectins (apple pectin AU-701, tanacetan) into the composition of a cryoprotective solution for platelets during low-temperature storage.					
36629825	2	32	dep	pectins	316:322	arg1	AU-701					338:343	apple pectin AU-701	325:343	apple pectin AU-701	325:343	OBJECTIVE To investigate the possibility of including pectins (apple pectin AU-701, tanacetan) into the composition of a cryoprotective solution for platelets during low-temperature storage.					
36629825	3	33	theme	concentrates	495:506	arg1	MATERIALS					453:461	MATERIALS	453:461	MATERIALS	453:461	MATERIALS AND METHODS Samples of platelet concentrates (PC) were frozen under the protection of complex solutions and stored in an electric freezer at -80 degree C for 1 and 6 months.					
36629825	3	33	theme	concentrates	495:506	arg1	METHODS					467:473	METHODS	467:473	METHODS	467:473	MATERIALS AND METHODS Samples of platelet concentrates (PC) were frozen under the protection of complex solutions and stored in an electric freezer at -80 degree C for 1 and 6 months.					
36629825	4	34	theme	cryoprotectants	679:693	arg1	DMAC					766:769	DMAC	766:769	DMAC	766:769	RESULT The study showed that of the basic cryoprotectants, the best effect in the preservation of PC was with dimethylacetamide (DMAC).					
36629825	4	34	theme	cryoprotectants	679:693	arg1	dimethylacetamide					747:763	dimethylacetamide	747:763	dimethylacetamide (DMAC)	747:770	RESULT The study showed that of the basic cryoprotectants, the best effect in the preservation of PC was with dimethylacetamide (DMAC).					
36629825	4	35	theme	best	700:703	arg1	effect					705:710	the best effect	696:710	the best effect in the preservation of PC	696:736	RESULT The study showed that of the basic cryoprotectants, the best effect in the preservation of PC was with dimethylacetamide (DMAC).					
36629825	7	36	theme	cryoprotective	1157:1170	arg1	action					1172:1177	its cryoprotective action	1153:1177	its cryoprotective action	1153:1177	Moreover, the chemical structure of pectin determines the level of its cryoprotective action in relation to the preservation of PC.					
36629825	1	37	theme	BACKGROUND	110:119	arg1	Pectins					121:127	BACKGROUND Pectins	110:127	BACKGROUND Pectins	110:127	BACKGROUND Pectins have unique properties and great potential to become an indispensable component of cryoprotective environment for platelet freezing.					
36629825	6	38	theme	combined	1045:1052	arg1	cryoprotectant					1054:1067	a new combined cryoprotectant	1039:1067	a new combined cryoprotectant for PC freezing	1039:1083	CONCLUSIONS We conclude that DMAC is more promising as a basis for the development of a new combined cryoprotectant for PC freezing.					
36629825	1	39	theme	cryoprotective	212:225	arg1	environment					227:237	cryoprotective environment	212:237	cryoprotective environment	212:237	BACKGROUND Pectins have unique properties and great potential to become an indispensable component of cryoprotective environment for platelet freezing.					
36629825	6	40	theme	PC	1073:1074	arg1	freezing					1076:1083	PC freezing	1073:1083	PC freezing	1073:1083	CONCLUSIONS We conclude that DMAC is more promising as a basis for the development of a new combined cryoprotectant for PC freezing.					
36629825	4	41	theme	PC	735:736	arg1	preservation					719:730	the preservation	715:730	the preservation of PC	715:736	RESULT The study showed that of the basic cryoprotectants, the best effect in the preservation of PC was with dimethylacetamide (DMAC).					
36629825	2	42	theme	pectin	331:336	arg1	tanacetan					346:354	tanacetan	346:354	tanacetan	346:354	OBJECTIVE To investigate the possibility of including pectins (apple pectin AU-701, tanacetan) into the composition of a cryoprotective solution for platelets during low-temperature storage.					
36629825	2	42	theme	pectin	331:336	arg1	AU-701					338:343	apple pectin AU-701	325:343	apple pectin AU-701	325:343	OBJECTIVE To investigate the possibility of including pectins (apple pectin AU-701, tanacetan) into the composition of a cryoprotective solution for platelets during low-temperature storage.					
36629825	1	43	theme	environment	227:237	arg1	component					199:207	an indispensable component	182:207	an indispensable component of cryoprotective environment for platelet freezing	182:259	BACKGROUND Pectins have unique properties and great potential to become an indispensable component of cryoprotective environment for platelet freezing.					
36629825	0	44	theme	solution	47:54	arg1	part					22:25	part	22:25	part of a cryoprotective solution for long-term storage of human platelet concentrates	22:107	The use of pectins as part of a cryoprotective solution for long-term storage of human platelet concentrates.					
36629825	3	45	theme	degree	608:613	arg1	C					615:615	-80 degree C	604:615	-80 degree C	604:615	MATERIALS AND METHODS Samples of platelet concentrates (PC) were frozen under the protection of complex solutions and stored in an electric freezer at -80 degree C for 1 and 6 months.					
36629825	5	46	theme	pectins	784:790	arg1	use					777:779	The use	773:779	The use of pectins as an additive to the base solution of DMAC	773:834	The use of pectins as an additive to the base solution of DMAC statistically improves the preservation of PC after exposure to low temperatures (-80 degree C) for 30 and 180 days.					
36629825	5	47	theme	DMAC	831:834	arg1	solution					819:826	the base solution	810:826	the base solution of DMAC	810:834	The use of pectins as an additive to the base solution of DMAC statistically improves the preservation of PC after exposure to low temperatures (-80 degree C) for 30 and 180 days.					
36629825	0	48	theme	cryoprotective	32:45	arg1	solution					47:54	a cryoprotective solution	30:54	a cryoprotective solution for long-term storage of human platelet concentrates	30:107	The use of pectins as part of a cryoprotective solution for long-term storage of human platelet concentrates.					
36629825	6	49	theme	new	1041:1043	arg1	cryoprotectant					1054:1067	a new combined cryoprotectant	1039:1067	a new combined cryoprotectant for PC freezing	1039:1083	CONCLUSIONS We conclude that DMAC is more promising as a basis for the development of a new combined cryoprotectant for PC freezing.					
36629825	4	50	dep	RESULT	637:642	arg1	showed					654:659	showed	654:659	showed that of the basic cryoprotectants, the best effect in the preservation of PC was with dimethylacetamide (DMAC)	654:770	RESULT The study showed that of the basic cryoprotectants, the best effect in the preservation of PC was with dimethylacetamide (DMAC).					
36629825	1	51	theme	unique	134:139	arg1	properties					141:150	unique properties	134:150	unique properties	134:150	BACKGROUND Pectins have unique properties and great potential to become an indispensable component of cryoprotective environment for platelet freezing.					
36629825	1	52	theme	platelet	243:250	arg1	freezing					252:259	platelet freezing	243:259	platelet freezing	243:259	BACKGROUND Pectins have unique properties and great potential to become an indispensable component of cryoprotective environment for platelet freezing.					
35689117	0	0	theme	supramolecular	103:116	arg1	assembly					118:125	hexadecyl trimethyl ammonium bromide-induced supramolecular assembly	58:125	hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles	58:169	Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles.					
35689117	1	1	theme	nanoparticles	398:410	arg1	assembly					359:366	hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly	292:366	hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs)	292:423	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	3	2	theme	host-guest	735:744	arg1	reaction					756:763	the host-guest chemistry reaction	731:763	the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change	731:868	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35689117	1	3	theme	hexadecyl	292:300	arg1	bromide					321:327	hexadecyl trimethyl ammonium bromide	292:327	hexadecyl trimethyl ammonium bromide	292:327	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	0	4	theme	bromide-induced	87:101	arg1	assembly					118:125	hexadecyl trimethyl ammonium bromide-induced supramolecular assembly	58:125	hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles	58:169	Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles.					
35689117	4	5	from	650	898:900	arg1	absorption					884:893	absorption	884:893	absorption at 650 and 520 nm (A650nm/A520nm)	884:927	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	4	5	from	650	898:900	arg1	ratio					875:879	The ratio	871:879	The ratio of absorption at 650 and 520 nm (A650nm/A520nm)	871:927	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	6	6	theme	potential	1226:1234	arg1	application					1236:1246	its potential application	1222:1246	its potential application in real samples	1222:1262	In milk samples, the recovery was 93.55-111.32%, which suggested its potential application in real samples.					
35689117	1	7	theme	specific	202:209	arg1	strategy					224:231	an effective and specific colorimetric strategy	185:231	an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs)	185:423	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	1	8	theme	trimethyl	302:310	arg1	bromide					321:327	hexadecyl trimethyl ammonium bromide	292:327	hexadecyl trimethyl ammonium bromide	292:327	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	4	9	theme	log	961:963	arg1	scale					965:969	the log scale	957:969	the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL	957:1080	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	5	10	theme	good	1120:1123	arg1	selectivity					1125:1135	good selectivity	1120:1135	good selectivity	1120:1135	In addition, this optical sensor has good selectivity and practicability.					
35689117	1	11	theme	ammonium	312:319	arg1	bromide					321:327	hexadecyl trimethyl ammonium bromide	292:327	hexadecyl trimethyl ammonium bromide	292:327	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	3	12	theme	chemistry	746:754	arg1	reaction					756:763	the host-guest chemistry reaction	731:763	the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change	731:868	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35689117	6	13	theme	real	1251:1254	arg1	samples					1256:1262	real samples	1251:1262	real samples	1251:1262	In milk samples, the recovery was 93.55-111.32%, which suggested its potential application in real samples.					
35689117	4	14	theme	bacteria	999:1006	arg1	concentration					978:990	the concentration	974:990	the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL	974:1080	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	3	15	theme	color	857:861	arg1	change					863:868	an obvious color change	846:868	an obvious color change	846:868	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35689117	0	16	theme	gold	152:155	arg1	nanoparticles					157:169	β-cyclodextrin-capped gold nanoparticles	130:169	β-cyclodextrin-capped gold nanoparticles	130:169	Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles.					
35689117	6	17	theme	milk	1160:1163	arg1	samples					1165:1171	milk samples	1160:1171	milk samples	1160:1171	In milk samples, the recovery was 93.55-111.32%, which suggested its potential application in real samples.					
35689117	4	18	contain	has	929:931	arg2	correlation					942:952	a linear correlation	933:952	a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL	933:1080	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	4	18	contain	has	929:931	arg1	ratio					875:879	The ratio	871:879	The ratio of absorption at 650 and 520 nm (A650nm/A520nm)	871:927	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	1	19	theme	colorimetric	211:222	arg1	strategy					224:231	an effective and specific colorimetric strategy	185:231	an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs)	185:423	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	0	20	theme	β-cyclodextrin-capped	130:150	arg1	nanoparticles					157:169	β-cyclodextrin-capped gold nanoparticles	130:169	β-cyclodextrin-capped gold nanoparticles	130:169	Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles.					
35689117	4	21	from	ratio	875:879	arg1	650					898:900	650	898:900	650	898:900	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	4	21	from	ratio	875:879	arg1	A650nm/A520nm					914:926	A650nm/A520nm	914:926	A650nm/A520nm	914:926	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	4	21	from	ratio	875:879	arg1	520 nm					906:911	520 nm	906:911	520 nm	906:911	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	5	22	theme	optical	1101:1107	arg1	sensor					1109:1114	this optical sensor	1096:1114	this optical sensor	1096:1114	In addition, this optical sensor has good selectivity and practicability.					
35689117	1	23	dep	-induced	335:342	arg1	bromide					321:327	hexadecyl trimethyl ammonium bromide	292:327	hexadecyl trimethyl ammonium bromide	292:327	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	2	24	theme	ssDNA	441:445	arg1	aptamer					447:453	ssDNA aptamer	441:453	ssDNA aptamer of S. typhimurium	441:471	In this study, ssDNA aptamer of S. typhimurium could combine with CTAB to form the supramolecular ssDNA-CTAB composite, so the ssDNA aptamer was applied to control the concentration of CTAB.					
35689117	3	25	theme	CTAB	768:771	arg1	reaction					756:763	the host-guest chemistry reaction	731:763	the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change	731:868	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35689117	4	26	theme	absorption	884:893	arg1	ratio					875:879	The ratio	871:879	The ratio of absorption at 650 and 520 nm (A650nm/A520nm)	871:927	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	4	27	theme	concentration	978:990	arg1	scale					965:969	the log scale	957:969	the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL	957:1080	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	0	28	theme	Colorimetric	0:11	arg1	detection					13:21	Colorimetric detection	0:21	Colorimetric detection of Salmonella typhimurium	0:47	Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles.					
35689117	3	29	theme	β-CD	777:780	arg1	reaction					756:763	the host-guest chemistry reaction	731:763	the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change	731:868	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35689117	4	30	theme	linear	935:940	arg1	correlation					942:952	a linear correlation	933:952	a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL	933:1080	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	4	31	with	bacteria	999:1006	arg1	limit					1044:1048	a low limit	1038:1048	a low limit of detection (LOD) of 13 CFU/mL	1038:1080	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	3	32	theme	obvious	849:855	arg1	change					863:868	an obvious color change	846:868	an obvious color change	846:868	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35689117	3	33	dep	typhimurium	639:649	arg1	the					620:622	the	620:622	the	620:622	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35689117	3	33	dep	typhimurium	639:649	arg1	presence					624:631	presence	624:631	presence	624:631	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35689117	4	34	theme	low	1040:1042	arg1	limit					1044:1048	a low limit	1038:1048	a low limit of detection (LOD) of 13 CFU/mL	1038:1080	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	0	35	theme	nanoparticles	157:169	arg1	assembly					118:125	hexadecyl trimethyl ammonium bromide-induced supramolecular assembly	58:125	hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles	58:169	Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles.					
35689117	2	36	theme	typhimurium	461:471	arg1	aptamer					447:453	ssDNA aptamer	441:453	ssDNA aptamer of S. typhimurium	441:471	In this study, ssDNA aptamer of S. typhimurium could combine with CTAB to form the supramolecular ssDNA-CTAB composite, so the ssDNA aptamer was applied to control the concentration of CTAB.					
35689117	2	37	theme	ssDNA-CTAB	524:533	arg1	composite					535:543	the supramolecular ssDNA-CTAB composite	505:543	the supramolecular ssDNA-CTAB composite	505:543	In this study, ssDNA aptamer of S. typhimurium could combine with CTAB to form the supramolecular ssDNA-CTAB composite, so the ssDNA aptamer was applied to control the concentration of CTAB.					
35689117	1	38	theme	-induced	335:342	arg1	assembly					359:366	hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly	292:366	hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs)	292:423	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	4	39	theme	13 CFU/mL	1072:1080	arg1	LOD					1064:1066	LOD	1064:1066	LOD	1064:1066	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	4	39	theme	13 CFU/mL	1072:1080	arg1	detection					1053:1061	detection	1053:1061	detection (LOD) of 13 CFU/mL	1053:1080	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	0	40	theme	typhimurium	37:47	arg1	detection					13:21	Colorimetric detection	0:21	Colorimetric detection of Salmonella typhimurium	0:47	Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles.					
35689117	1	41	theme	supramolecular	344:357	arg1	assembly					359:366	hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly	292:366	hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs)	292:423	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	2	42	theme	supramolecular	509:522	arg1	composite					535:543	the supramolecular ssDNA-CTAB composite	505:543	the supramolecular ssDNA-CTAB composite	505:543	In this study, ssDNA aptamer of S. typhimurium could combine with CTAB to form the supramolecular ssDNA-CTAB composite, so the ssDNA aptamer was applied to control the concentration of CTAB.					
35689117	2	43	theme	ssDNA	553:557	arg1	aptamer					559:565	the ssDNA aptamer	549:565	the ssDNA aptamer	549:565	In this study, ssDNA aptamer of S. typhimurium could combine with CTAB to form the supramolecular ssDNA-CTAB composite, so the ssDNA aptamer was applied to control the concentration of CTAB.					
35689117	0	44	theme	hexadecyl	58:66	arg1	assembly					118:125	hexadecyl trimethyl ammonium bromide-induced supramolecular assembly	58:125	hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles	58:169	Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles.					
35689117	3	45	theme	β-CD-AuNP	795:803	arg1	aggregation					829:839	β-CD-AuNP supramolecular assembly aggregation	795:839	β-CD-AuNP supramolecular assembly aggregation	795:839	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35689117	5	46	contain	has	1116:1118	arg1	sensor					1109:1114	this optical sensor	1096:1114	this optical sensor	1096:1114	In addition, this optical sensor has good selectivity and practicability.					
35689117	5	46	contain	has	1116:1118	arg2	practicability					1141:1154	practicability	1141:1154	practicability	1141:1154	In addition, this optical sensor has good selectivity and practicability.					
35689117	5	46	contain	has	1116:1118	arg2	selectivity					1125:1135	good selectivity	1120:1135	good selectivity	1120:1135	In addition, this optical sensor has good selectivity and practicability.					
35689117	4	47	from	520 nm	906:911	arg1	absorption					884:893	absorption	884:893	absorption at 650 and 520 nm (A650nm/A520nm)	884:927	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	4	47	from	520 nm	906:911	arg1	ratio					875:879	The ratio	871:879	The ratio of absorption at 650 and 520 nm (A650nm/A520nm)	871:927	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	6	48	from	application	1236:1246	arg1	samples					1256:1262	real samples	1251:1262	real samples	1251:1262	In milk samples, the recovery was 93.55-111.32%, which suggested its potential application in real samples.					
35689117	1	49	theme	β-cyclodextrin-capped	371:391	arg1	β-CD-AuNPs					413:422	β-CD-AuNPs	413:422	β-CD-AuNPs	413:422	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	1	49	theme	β-cyclodextrin-capped	371:391	arg1	nanoparticles					398:410	β-cyclodextrin-capped gold nanoparticles	371:410	β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs)	371:423	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	2	50	theme	CTAB	611:614	arg1	concentration					594:606	the concentration	590:606	the concentration of CTAB	590:614	In this study, ssDNA aptamer of S. typhimurium could combine with CTAB to form the supramolecular ssDNA-CTAB composite, so the ssDNA aptamer was applied to control the concentration of CTAB.					
35689117	3	51	theme	ssDNA	652:656	arg1	aptamers					658:665	ssDNA aptamers	652:665	ssDNA aptamers	652:665	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35689117	6	52	from	%	1203:1203	arg1	samples					1165:1171	milk samples	1160:1171	milk samples	1160:1171	In milk samples, the recovery was 93.55-111.32%, which suggested its potential application in real samples.					
35689117	0	53	theme	ammonium	78:85	arg1	assembly					118:125	hexadecyl trimethyl ammonium bromide-induced supramolecular assembly	58:125	hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles	58:169	Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles.					
35689117	3	54	with	reaction	756:763	arg1	change					863:868	an obvious color change	846:868	an obvious color change	846:868	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35689117	3	55	theme	assembly	820:827	arg1	aggregation					829:839	β-CD-AuNP supramolecular assembly aggregation	795:839	β-CD-AuNP supramolecular assembly aggregation	795:839	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35689117	0	56	theme	trimethyl	68:76	arg1	assembly					118:125	hexadecyl trimethyl ammonium bromide-induced supramolecular assembly	58:125	hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles	58:169	Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles.					
35689117	1	57	theme	gold	393:396	arg1	β-CD-AuNPs					413:422	β-CD-AuNPs	413:422	β-CD-AuNPs	413:422	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	1	57	theme	gold	393:396	arg1	nanoparticles					398:410	β-cyclodextrin-capped gold nanoparticles	371:410	β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs)	371:423	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	1	58	dep	typhimurium	254:264	arg1	typhimurium					270:280	S. typhimurium	267:280	S. typhimurium	267:280	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	1	59	theme	effective	188:196	arg1	strategy					224:231	an effective and specific colorimetric strategy	185:231	an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs)	185:423	We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs).					
35689117	4	60	theme	detection	1053:1061	arg1	limit					1044:1048	a low limit	1038:1048	a low limit of detection (LOD) of 13 CFU/mL	1038:1080	The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL.					
35689117	3	61	theme	supramolecular	805:818	arg1	aggregation					829:839	β-CD-AuNP supramolecular assembly aggregation	795:839	β-CD-AuNP supramolecular assembly aggregation	795:839	In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change.					
35581061	0	0	theme	macrophage	100:109	arg1	polarization					111:122	macrophage polarization	100:122	macrophage polarization: An in vivo study	100:140	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries enhances macrophage polarization: An in vivo study.					
35581061	6	1	theme	surface	1315:1321	arg1	regularity					1323:1332	the significant surface regularity	1299:1332	the significant surface regularity	1299:1332	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	6	2	with	hydrogel	1431:1438	arg1	healing					1452:1458	tissue healing	1445:1458	tissue healing at the end of 12 weeks	1445:1481	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	1	3	theme	cartilage	256:264	arg1	layer					266:270	the cartilage layer	252:270	the cartilage layer	252:270	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	6	4	theme	tissue	1445:1450	arg1	healing					1452:1458	tissue healing	1445:1458	tissue healing at the end of 12 weeks	1445:1481	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	2	5	theme	±	596:596	arg1	kPa					602:604	469.7 ± 6.1 kPa	590:604	469.7 ± 6.1 kPa	590:604	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	2	5	theme	±	596:596	arg1	hydrogel					580:587	XLS/Alg-PAAm hydrogel	567:587	XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa)	567:605	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	1	6	dep	in	436:437	arg1	vivo					439:442	vivo	439:442	vivo	439:442	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	2	7	theme	±	543:543	arg1	hydrogels					556:564	Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels	494:564	Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels	494:564	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	2	7	theme	±	543:543	arg1	kPa					551:553	317.5 ± 21.72 kPa	537:553	317.5 ± 21.72 kPa	537:553	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	1	8	theme	in	423:424	arg1	experiments					444:454	in vitro and in vivo experiments	423:454	in vitro and in vivo experiments	423:454	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	0	9	dep	in	128:129	arg1	vivo					131:134	vivo	131:134	vivo	131:134	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries enhances macrophage polarization: An in vivo study.					
35581061	5	10	theme	cell	1192:1195	arg1	viability					1197:1205	a better cell viability	1183:1205	a better cell viability as well as repairment thorough the XLS contents	1183:1253	Moreover, bilayer structure exhibited a better cell viability as well as repairment thorough the XLS contents.					
35581061	3	11	theme	polarization	770:781	arg1	transition					745:754	transition	745:754	transition	745:754	The addition of TGF-β3 accelerated transition of macrophage polarization, especially between day 4 and 7.					
35581061	7	12	theme	durable	1499:1505	arg1	hydrogel					1543:1550	A mechanically durable, biocompatible and immunocompatible hydrogel	1484:1550	A mechanically durable, biocompatible and immunocompatible hydrogel	1484:1550	A mechanically durable, biocompatible and immunocompatible hydrogel was formulated to be utilized in bone-cartilage engineering applications.					
35581061	6	13	theme	formation	1358:1366	arg1	employment					1368:1377	tissue formation employment	1351:1377	tissue formation employment	1351:1377	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	4	14	theme	expression	820:829	arg1	levels					831:836	The expression levels	816:836	The expression levels of M1-related genes such as CD80, iNOS and TNF-α	816:885	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	7	15	theme	mechanically	1486:1497	arg1	hydrogel					1543:1550	A mechanically durable, biocompatible and immunocompatible hydrogel	1484:1550	A mechanically durable, biocompatible and immunocompatible hydrogel	1484:1550	A mechanically durable, biocompatible and immunocompatible hydrogel was formulated to be utilized in bone-cartilage engineering applications.					
35581061	0	16	theme	in	128:129	arg1	study					136:140	An in vivo study	125:140	macrophage polarization: An in vivo study	100:140	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries enhances macrophage polarization: An in vivo study.					
35581061	2	17	theme	XLS/Alg-PAAm	567:578	arg1	kPa					602:604	469.7 ± 6.1 kPa	590:604	469.7 ± 6.1 kPa	590:604	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	2	17	theme	XLS/Alg-PAAm	567:578	arg1	hydrogel					580:587	XLS/Alg-PAAm hydrogel	567:587	XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa)	567:605	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	2	18	theme	factors	701:707	arg1	expression					669:678	the expression	665:678	the expression of anti-inflammatory factors	665:707	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	6	19	theme	histological	1264:1275	arg1	examinations					1277:1288	In vivo histological examinations	1256:1288	In vivo histological examinations	1256:1288	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	4	20	theme	lowest	929:934	arg1	values					936:941	lowest values	929:941	lowest values	929:941	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	1	21	theme	in	436:437	arg1	experiments					444:454	in vitro and in vivo experiments	423:454	in vitro and in vivo experiments	423:454	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	0	22	dep	polarization	111:122	arg1	study					136:140	An in vivo study	125:140	macrophage polarization: An in vivo study	100:140	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries enhances macrophage polarization: An in vivo study.					
35581061	2	23	theme	anti-inflammatory	683:699	arg1	factors					701:707	anti-inflammatory factors	683:707	anti-inflammatory factors	683:707	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	2	24	theme	Alg-PAAm	494:501	arg1	hydrogels					556:564	Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels	494:564	Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels	494:564	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	2	24	theme	Alg-PAAm	494:501	arg1	kPa					551:553	317.5 ± 21.72 kPa	537:553	317.5 ± 21.72 kPa	537:553	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	0	25	theme	laponite/alginate-poly	10:31	arg1	hydrogel					55:62	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel	0:62	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries	0:89	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries enhances macrophage polarization: An in vivo study.					
35581061	1	26	theme	TGF-β3	356:361	arg1	addition					344:351	the addition	340:351	the addition of TGF-β3 and bone layer	340:376	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	2	27	theme	hydrogels	556:564	arg1	strength					482:489	the mechanical strength	467:489	the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels	467:564	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	4	28	theme	accelerated	1120:1130	arg1	repair					1137:1142	accelerated bone repair	1120:1142	accelerated bone repair	1120:1142	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	6	29	theme	degradation	1404:1414	arg1	profile					1416:1422	synchronized degradation profile	1391:1422	synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks	1391:1481	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	1	30	theme	current	194:200	arg1	study					202:206	current study	194:206	current study	194:206	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	0	31	theme	Bilayered	0:8	arg1	hydrogel					55:62	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel	0:62	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries	0:89	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries enhances macrophage polarization: An in vivo study.					
35581061	7	32	theme	bone-cartilage	1585:1598	arg1	applications					1612:1623	bone-cartilage engineering applications	1585:1623	bone-cartilage engineering applications	1585:1623	A mechanically durable, biocompatible and immunocompatible hydrogel was formulated to be utilized in bone-cartilage engineering applications.					
35581061	5	33	theme	repairment	1218:1227	arg1	contents					1246:1253	the XLS contents	1238:1253	a better cell viability as well as repairment thorough the XLS contents	1183:1253	Moreover, bilayer structure exhibited a better cell viability as well as repairment thorough the XLS contents.					
35581061	1	34	theme	study	202:206	arg1	objective					181:189	the objective	177:189	the objective of current study	177:206	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	0	35	theme	acrylamide	33:42	arg1	hydrogel					55:62	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel	0:62	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries	0:89	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries enhances macrophage polarization: An in vivo study.					
35581061	7	36	theme	engineering	1600:1610	arg1	applications					1612:1623	bone-cartilage engineering applications	1585:1623	bone-cartilage engineering applications	1585:1623	A mechanically durable, biocompatible and immunocompatible hydrogel was formulated to be utilized in bone-cartilage engineering applications.					
35581061	2	37	dep	Alg-PAAm	494:501	arg1	kPa					517:519	32.95 ± 1.23 kPa	504:519	32.95 ± 1.23 kPa	504:519	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	6	38	from	end	1467:1469	arg1	healing					1452:1458	tissue healing	1445:1458	tissue healing at the end of 12 weeks	1445:1481	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	6	39	theme	significant	1303:1313	arg1	regularity					1323:1332	the significant surface regularity	1299:1332	the significant surface regularity	1299:1332	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	5	40	theme	thorough	1229:1236	arg1	contents					1246:1253	the XLS contents	1238:1253	a better cell viability as well as repairment thorough the XLS contents	1183:1253	Moreover, bilayer structure exhibited a better cell viability as well as repairment thorough the XLS contents.					
35581061	4	41	dep	decreased	887:895	arg1	whereas					954:960	whereas	954:960	whereas	954:960	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	5	42	theme	better	1185:1190	arg1	viability					1197:1205	a better cell viability	1183:1205	a better cell viability as well as repairment thorough the XLS contents	1183:1253	Moreover, bilayer structure exhibited a better cell viability as well as repairment thorough the XLS contents.					
35581061	0	43	theme	composite	45:53	arg1	hydrogel					55:62	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel	0:62	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries	0:89	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries enhances macrophage polarization: An in vivo study.					
35581061	6	44	theme	weeks	1477:1481	arg1	end					1467:1469	the end	1463:1469	the end of 12 weeks	1463:1481	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	1	45	theme	bone	367:370	arg1	layer					372:376	bone layer	367:376	bone layer	367:376	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	5	46	theme	XLS	1242:1244	arg1	contents					1246:1253	the XLS contents	1238:1253	a better cell viability as well as repairment thorough the XLS contents	1183:1253	Moreover, bilayer structure exhibited a better cell viability as well as repairment thorough the XLS contents.					
35581061	6	47	theme	tissue	1351:1356	arg1	employment					1368:1377	tissue formation employment	1351:1377	tissue formation employment	1351:1377	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	2	48	theme	±	510:510	arg1	kPa					517:519	32.95 ± 1.23 kPa	504:519	32.95 ± 1.23 kPa	504:519	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	1	49	theme	layer	372:376	arg1	addition					344:351	the addition	340:351	the addition of TGF-β3 and bone layer	340:376	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	7	50	theme	biocompatible	1508:1520	arg1	hydrogel					1543:1550	A mechanically durable, biocompatible and immunocompatible hydrogel	1484:1550	A mechanically durable, biocompatible and immunocompatible hydrogel	1484:1550	A mechanically durable, biocompatible and immunocompatible hydrogel was formulated to be utilized in bone-cartilage engineering applications.					
35581061	1	51	theme	bilayered	226:234	arg1	hydrogel					236:243	bilayered hydrogel	226:243	bilayered hydrogel	226:243	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	1	52	theme	-polyacrylamide	300:314	arg1	Alg					296:298	Alg	296:298	Alg	296:298	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	1	52	theme	-polyacrylamide	300:314	arg1	alginate					286:293	alginate (Alg)-polyacrylamide (PAAm)	286:321	alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer	286:376	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	1	52	theme	-polyacrylamide	300:314	arg1	PAAm					317:320	PAAm	317:320	PAAm	317:320	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	4	53	theme	M1-related	841:850	arg1	genes					852:856	M1-related genes	841:856	M1-related genes such as CD80, iNOS and TNF-α	841:885	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	4	53	theme	M1-related	841:850	arg1	CD80					866:869	CD80	866:869	CD80	866:869	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	4	53	theme	M1-related	841:850	arg1	TNF-α					881:885	TNF-α	881:885	TNF-α	881:885	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	4	53	theme	M1-related	841:850	arg1	iNOS					872:875	iNOS	872:875	iNOS	872:875	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	2	54	theme	mechanical	471:480	arg1	strength					482:489	the mechanical strength	467:489	the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels	467:564	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	7	55	used	utilized	1573:1580	arg2	hydrogel					1543:1550	A mechanically durable, biocompatible and immunocompatible hydrogel	1484:1550	A mechanically durable, biocompatible and immunocompatible hydrogel	1484:1550	A mechanically durable, biocompatible and immunocompatible hydrogel was formulated to be utilized in bone-cartilage engineering applications.					
35581061	2	56	theme	M2	637:638	arg1	phenotype					640:648	M2 phenotype	637:648	M2 phenotype	637:648	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	6	57	dep	In	1256:1257	arg1	vivo					1259:1262	vivo	1259:1262	vivo	1259:1262	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	2	58	theme	based	530:534	arg1	hydrogels					556:564	Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels	494:564	Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels	494:564	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	2	58	theme	based	530:534	arg1	kPa					551:553	317.5 ± 21.72 kPa	537:553	317.5 ± 21.72 kPa	537:553	Exceeding the mechanical strength of Alg-PAAm (32.95 ± 1.23 kPa) and XLS based (317.5 ± 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 ± 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors.					
35581061	1	59	theme	laponite	381:388	arg1	XLS/Alg-PAAm					390:401	laponite XLS/Alg-PAAm	381:401	laponite XLS/Alg-PAAm	381:401	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	4	60	theme	genes	998:1002	arg1	levels					977:982	the expression levels	962:982	the expression levels of M2-related genes, CD206, Arg1 and STAT6	962:1025	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	4	61	theme	bone	1132:1135	arg1	repair					1137:1142	accelerated bone repair	1120:1142	accelerated bone repair	1120:1142	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	4	62	theme	M2-related	987:996	arg1	genes					998:1002	M2-related genes	987:1002	M2-related genes	987:1002	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	4	62	theme	M2-related	987:996	arg1	STAT6					1021:1025	STAT6	1021:1025	STAT6	1021:1025	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	4	62	theme	M2-related	987:996	arg1	Arg1					1012:1015	Arg1	1012:1015	Arg1	1012:1015	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	4	62	theme	M2-related	987:996	arg1	CD206					1005:1009	CD206	1005:1009	CD206	1005:1009	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	7	63	theme	immunocompatible	1526:1541	arg1	hydrogel					1543:1550	A mechanically durable, biocompatible and immunocompatible hydrogel	1484:1550	A mechanically durable, biocompatible and immunocompatible hydrogel	1484:1550	A mechanically durable, biocompatible and immunocompatible hydrogel was formulated to be utilized in bone-cartilage engineering applications.					
35581061	4	64	theme	genes	852:856	arg1	levels					831:836	The expression levels	816:836	The expression levels of M1-related genes such as CD80, iNOS and TNF-α	816:885	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	6	65	theme	In	1256:1257	arg1	examinations					1277:1288	In vivo histological examinations	1256:1288	In vivo histological examinations	1256:1288	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	4	66	theme	M2	1061:1062	arg1	polarization					1075:1086	M2 macrophage polarization	1061:1086	M2 macrophage polarization	1061:1086	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	6	67	theme	hydrogel	1431:1438	arg1	profile					1416:1422	synchronized degradation profile	1391:1422	synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks	1391:1481	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	3	68	theme	macrophage	759:768	arg1	polarization					770:781	macrophage polarization	759:781	macrophage polarization	759:781	The addition of TGF-β3 accelerated transition of macrophage polarization, especially between day 4 and 7.					
35581061	6	69	theme	synchronized	1391:1402	arg1	profile					1416:1422	synchronized degradation profile	1391:1422	synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks	1391:1481	In vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks.					
35581061	4	70	theme	macrophage	1064:1073	arg1	polarization					1075:1086	M2 macrophage polarization	1061:1086	M2 macrophage polarization	1061:1086	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	1	71	theme	osteochondral	154:166	arg1	defects					168:174	osteochondral defects	154:174	osteochondral defects	154:174	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	1	72	dep	in	423:424	arg1	vitro					426:430	vitro	426:430	vitro	426:430	Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments.					
35581061	5	73	theme	bilayer	1155:1161	arg1	structure					1163:1171	bilayer structure	1155:1171	bilayer structure	1155:1171	Moreover, bilayer structure exhibited a better cell viability as well as repairment thorough the XLS contents.					
35581061	0	74	theme	osteochondral	68:80	arg1	injuries					82:89	osteochondral injuries	68:89	osteochondral injuries	68:89	Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries enhances macrophage polarization: An in vivo study.					
35581061	4	75	theme	expression	966:975	arg1	levels					977:982	the expression levels	962:982	the expression levels of M2-related genes, CD206, Arg1 and STAT6	962:1025	The expression levels of M1-related genes such as CD80, iNOS and TNF-α decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Arg1 and STAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair.					
35581061	3	76	theme	TGF-β3	726:731	arg1	addition					714:721	The addition	710:721	The addition of TGF-β3	710:731	The addition of TGF-β3 accelerated transition of macrophage polarization, especially between day 4 and 7.					
35726799	2	0	theme	old	472:474	arg1	layers					488:493	140 four weeks old Hisex Brown layers	457:493	140 four weeks old Hisex Brown layers	457:493	For the experimental feeding period of 8 weeks, 140 four weeks old Hisex Brown layers were divided into 10 treatment groups, comprising 14 birds each.					
35726799	1	1	theme	laying	320:325	arg1	performance					327:337	laying performance	320:337	laying performance	320:337	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	9	2	theme	high	1297:1300	arg1	chitosan					1315:1322	high deacetylated chitosan	1297:1322	high deacetylated chitosan by increasing chitosan level	1297:1351	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	4	3	theme	egg	720:722	arg1	quality					724:730	egg quality	720:730	egg quality	720:730	Performance was assessed by recording feed intake, egg weight, daily egg production, egg quality and egg biochemistry.					
35726799	13	4	theme	chitosan	1892:1899	arg1	supplementation					1901:1915	chitosan supplementation	1892:1915	chitosan supplementation	1892:1915	However, feed conversion ratio and faeces viscosity were impaired by increasing levels of chitosan supplementation, and lightness of yolk was increased by supplementation of chitosan with a high degree of deacetylation.					
35726799	12	5	theme	dietary	1676:1682	arg1	level					1684:1688	dietary level	1676:1688	dietary level	1676:1688	In conclusion, dietary level or different degrees of deacetylated chitosan may reduce yolk cholesterol and improve serum antioxidant status.					
35726799	2	6	theme	Brown	482:486	arg1	layers					488:493	140 four weeks old Hisex Brown layers	457:493	140 four weeks old Hisex Brown layers	457:493	For the experimental feeding period of 8 weeks, 140 four weeks old Hisex Brown layers were divided into 10 treatment groups, comprising 14 birds each.					
35726799	10	7	theme	deacetylation	1486:1498	arg1	degree					1476:1481	degree	1476:1481	degree of deacetylation and chitosan level	1476:1517	A significant interaction between degree of deacetylation and chitosan level was determined for serum glucose and calcium concentration.					
35726799	10	8	dep	deacetylation	1486:1498	arg1	level					1513:1517	level	1513:1517	level	1513:1517	A significant interaction between degree of deacetylation and chitosan level was determined for serum glucose and calcium concentration.					
35726799	1	9	theme	egg	358:360	arg1	quality					362:368	egg quality	358:368	egg quality	358:368	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	2	10	theme	feeding	430:436	arg1	period					438:443	the experimental feeding period	413:443	the experimental feeding period of 8 weeks	413:454	For the experimental feeding period of 8 weeks, 140 four weeks old Hisex Brown layers were divided into 10 treatment groups, comprising 14 birds each.					
35726799	3	11	theme	individual	585:594	arg1	cages					596:600	individual cages	585:600	individual cages	585:600	The birds were housed in individual cages in a complete randomised design.					
35726799	0	12	theme	serum	100:104	arg1	biochemistry					106:117	serum biochemistry	100:117	serum biochemistry	100:117	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	6	13	theme	faeces	920:925	arg1	viscosity					927:935	faeces viscosity	920:935	faeces viscosity	920:935	Feed conversion ratio and faeces viscosity were deteriorated by increased level of chitosan.					
35726799	5	14	theme	Serum	754:758	arg1	parameters					773:782	Serum biochemistry parameters	754:782	Serum biochemistry parameters	754:782	Serum biochemistry parameters were determined at the beginning and end of the experiment and faeces viscosity at the end of the experiment.					
35726799	8	15	theme	egg	1152:1154	arg1	yolk					1156:1159	egg yolk	1152:1159	egg yolk	1152:1159	Yellowness of egg yolk was affected by interaction of deacetylation degree and level of chitosan.					
35726799	7	16	theme	egg	1000:1002	arg1	yolk					1004:1007	egg yolk	1000:1007	egg yolk	1000:1007	Lightness of egg yolk was significantly increased in animals receiving high-degree deacetylated chitosan compared to low-degree deacetylated chitosan.					
35726799	1	17	theme	deacetylation	191:203	arg1	degree					205:210	the deacetylation degree	187:210	the deacetylation degree of chitosan (low: 70% vs. high: 90%)	187:247	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	10	18	theme	chitosan	1504:1511	arg1	degree					1476:1481	degree	1476:1481	degree of deacetylation and chitosan level	1476:1517	A significant interaction between degree of deacetylation and chitosan level was determined for serum glucose and calcium concentration.					
35726799	3	19	theme	complete	607:614	arg1	design					627:632	a complete randomised design	605:632	a complete randomised design	605:632	The birds were housed in individual cages in a complete randomised design.					
35726799	0	20	theme	hens	129:132	arg1	performance					72:82	performance	72:82	performance	72:82	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	0	20	theme	hens	129:132	arg1	traits					89:94	egg traits	85:94	egg traits	85:94	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	0	20	theme	hens	129:132	arg1	biochemistry					106:117	serum biochemistry	100:117	serum biochemistry	100:117	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	13	21	theme	deacetylation	2007:2019	arg1	degree					1997:2002	a high degree	1990:2002	a high degree of deacetylation	1990:2019	However, feed conversion ratio and faeces viscosity were impaired by increasing levels of chitosan supplementation, and lightness of yolk was increased by supplementation of chitosan with a high degree of deacetylation.					
35726799	9	22	theme	low	1376:1378	arg1	chitosan					1393:1400	low deacetylated chitosan	1376:1400	low deacetylated chitosan	1376:1400	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	1	23	dep	%	232:232	arg1	%					246:246	90%	244:246	70% vs. high: 90%	230:246	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	5	24	dep	beginning	807:815	arg1	the					803:805	the	803:805	the	803:805	Serum biochemistry parameters were determined at the beginning and end of the experiment and faeces viscosity at the end of the experiment.					
35726799	5	25	theme	experiment	882:891	arg1	end					871:873	the end	867:873	the end of the experiment	867:891	Serum biochemistry parameters were determined at the beginning and end of the experiment and faeces viscosity at the end of the experiment.					
35726799	13	26	with	supplementation	1957:1971	arg1	degree					1997:2002	a high degree	1990:2002	a high degree of deacetylation	1990:2019	However, feed conversion ratio and faeces viscosity were impaired by increasing levels of chitosan supplementation, and lightness of yolk was increased by supplementation of chitosan with a high degree of deacetylation.					
35726799	5	27	theme	biochemistry	760:771	arg1	parameters					773:782	Serum biochemistry parameters	754:782	Serum biochemistry parameters	754:782	Serum biochemistry parameters were determined at the beginning and end of the experiment and faeces viscosity at the end of the experiment.					
35726799	11	28	theme	dietary	1643:1649	arg1	chitosan					1651:1658	dietary chitosan	1643:1658	dietary chitosan	1643:1658	Serum total antioxidant content increased with higher levels of dietary chitosan.					
35726799	1	29	theme	dietary	257:263	arg1	level					265:269	its dietary level	253:269	its dietary level (0, 200, 400, 800, 1600 mg/kg diet)	253:305	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	1	29	theme	dietary	257:263	arg1	diet					301:304	0, 200, 400, 800, 1600 mg/kg diet	272:304	0, 200, 400, 800, 1600 mg/kg diet	272:304	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	7	30	theme	yolk	1004:1007	arg1	Lightness					987:995	Lightness	987:995	Lightness of egg yolk	987:1007	Lightness of egg yolk was significantly increased in animals receiving high-degree deacetylated chitosan compared to low-degree deacetylated chitosan.					
35726799	8	31	theme	degree	1206:1211	arg1	level					1217:1221	level	1217:1221	level of chitosan	1217:1233	Yellowness of egg yolk was affected by interaction of deacetylation degree and level of chitosan.					
35726799	8	31	theme	degree	1206:1211	arg1	interaction					1177:1187	interaction	1177:1187	interaction of deacetylation degree	1177:1211	Yellowness of egg yolk was affected by interaction of deacetylation degree and level of chitosan.					
35726799	7	32	theme	deacetylated	1070:1081	arg1	chitosan					1083:1090	high-degree deacetylated chitosan	1058:1090	high-degree deacetylated chitosan	1058:1090	Lightness of egg yolk was significantly increased in animals receiving high-degree deacetylated chitosan compared to low-degree deacetylated chitosan.					
35726799	1	33	theme	present	139:145	arg1	study					147:151	The present study	135:151	The present study	135:151	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	0	34	from	Effects	0:6	arg1	performance					72:82	performance	72:82	performance	72:82	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	0	34	from	Effects	0:6	arg1	traits					89:94	egg traits	85:94	egg traits	85:94	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	0	34	from	Effects	0:6	arg1	biochemistry					106:117	serum biochemistry	100:117	serum biochemistry	100:117	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	13	35	theme	conversion	1816:1825	arg1	ratio					1827:1831	feed conversion ratio	1811:1831	feed conversion ratio	1811:1831	However, feed conversion ratio and faeces viscosity were impaired by increasing levels of chitosan supplementation, and lightness of yolk was increased by supplementation of chitosan with a high degree of deacetylation.					
35726799	9	36	theme	yolk	1424:1427	arg1	cholesterol					1429:1439	yolk cholesterol	1424:1439	yolk cholesterol	1424:1439	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	8	37	theme	chitosan	1226:1233	arg1	level					1217:1221	level	1217:1221	level of chitosan	1217:1233	Yellowness of egg yolk was affected by interaction of deacetylation degree and level of chitosan.					
35726799	8	37	theme	chitosan	1226:1233	arg1	interaction					1177:1187	interaction	1177:1187	interaction of deacetylation degree	1177:1211	Yellowness of egg yolk was affected by interaction of deacetylation degree and level of chitosan.					
35726799	13	38	theme	chitosan	1976:1983	arg1	supplementation					1957:1971	supplementation	1957:1971	supplementation of chitosan with a high degree of deacetylation	1957:2019	However, feed conversion ratio and faeces viscosity were impaired by increasing levels of chitosan supplementation, and lightness of yolk was increased by supplementation of chitosan with a high degree of deacetylation.					
35726799	10	39	theme	serum	1538:1542	arg1	glucose					1544:1550	serum glucose	1538:1550	serum glucose	1538:1550	A significant interaction between degree of deacetylation and chitosan level was determined for serum glucose and calcium concentration.					
35726799	6	40	theme	Feed	894:897	arg1	ratio					910:914	Feed conversion ratio	894:914	Feed conversion ratio	894:914	Feed conversion ratio and faeces viscosity were deteriorated by increased level of chitosan.					
35726799	0	41	theme	different	11:19	arg1	degrees					21:27	different degrees	11:27	different degrees of deacetylation	11:44	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	12	42	theme	serum	1776:1780	arg1	status					1794:1799	serum antioxidant status	1776:1799	serum antioxidant status	1776:1799	In conclusion, dietary level or different degrees of deacetylated chitosan may reduce yolk cholesterol and improve serum antioxidant status.					
35726799	4	43	theme	feed	673:676	arg1	intake					678:683	feed intake	673:683	feed intake	673:683	Performance was assessed by recording feed intake, egg weight, daily egg production, egg quality and egg biochemistry.					
35726799	12	44	theme	chitosan	1727:1734	arg1	degrees					1703:1709	different degrees	1693:1709	different degrees	1693:1709	In conclusion, dietary level or different degrees of deacetylated chitosan may reduce yolk cholesterol and improve serum antioxidant status.					
35726799	12	44	theme	chitosan	1727:1734	arg1	level					1684:1688	dietary level	1676:1688	dietary level	1676:1688	In conclusion, dietary level or different degrees of deacetylated chitosan may reduce yolk cholesterol and improve serum antioxidant status.					
35726799	7	45	dep	increased	1027:1035	arg1	compared					1092:1099	compared	1092:1099	compared to low-degree deacetylated chitosan	1092:1135	Lightness of egg yolk was significantly increased in animals receiving high-degree deacetylated chitosan compared to low-degree deacetylated chitosan.					
35726799	11	46	theme	antioxidant	1591:1601	arg1	content					1603:1609	Serum total antioxidant content	1579:1609	Serum total antioxidant content	1579:1609	Serum total antioxidant content increased with higher levels of dietary chitosan.					
35726799	9	47	theme	cholesterol	1241:1251	arg1	concentration					1253:1265	Yolk cholesterol concentration	1236:1265	Yolk cholesterol concentration	1236:1265	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	12	48	theme	yolk	1747:1750	arg1	cholesterol					1752:1762	yolk cholesterol	1747:1762	yolk cholesterol	1747:1762	In conclusion, dietary level or different degrees of deacetylated chitosan may reduce yolk cholesterol and improve serum antioxidant status.					
35726799	1	49	theme	serum	379:383	arg1	biochemistry					385:396	serum biochemistry	379:396	serum biochemistry	379:396	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	5	50	theme	experiment	832:841	arg1	viscosity					854:862	the experiment and faeces viscosity	828:862	the experiment and faeces viscosity	828:862	Serum biochemistry parameters were determined at the beginning and end of the experiment and faeces viscosity at the end of the experiment.					
35726799	12	51	theme	different	1693:1701	arg1	degrees					1703:1709	different degrees	1693:1709	different degrees	1693:1709	In conclusion, dietary level or different degrees of deacetylated chitosan may reduce yolk cholesterol and improve serum antioxidant status.					
35726799	11	52	theme	higher	1626:1631	arg1	levels					1633:1638	higher levels	1626:1638	higher levels of dietary chitosan	1626:1658	Serum total antioxidant content increased with higher levels of dietary chitosan.					
35726799	1	53	theme	chitosan	215:222	arg1	degree					205:210	the deacetylation degree	187:210	the deacetylation degree of chitosan (low: 70% vs. high: 90%)	187:247	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	1	53	theme	chitosan	215:222	arg1	level					265:269	its dietary level	253:269	its dietary level (0, 200, 400, 800, 1600 mg/kg diet)	253:305	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	1	53	theme	chitosan	215:222	arg1	diet					301:304	0, 200, 400, 800, 1600 mg/kg diet	272:304	0, 200, 400, 800, 1600 mg/kg diet	272:304	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	5	54	theme	faeces	847:852	arg1	viscosity					854:862	the experiment and faeces viscosity	828:862	the experiment and faeces viscosity	828:862	Serum biochemistry parameters were determined at the beginning and end of the experiment and faeces viscosity at the end of the experiment.					
35726799	4	55	theme	egg	704:706	arg1	production					708:717	daily egg production	698:717	daily egg production	698:717	Performance was assessed by recording feed intake, egg weight, daily egg production, egg quality and egg biochemistry.					
35726799	7	56	theme	low-degree	1104:1113	arg1	chitosan					1128:1135	low-degree deacetylated chitosan	1104:1135	low-degree deacetylated chitosan	1104:1135	Lightness of egg yolk was significantly increased in animals receiving high-degree deacetylated chitosan compared to low-degree deacetylated chitosan.					
35726799	1	57	dep	affect	313:318	arg1	faeces					340:345	faeces	340:345	faeces viscosity, egg quality, egg and serum biochemistry of layers	340:406	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	9	58	theme	deacetylated	1302:1313	arg1	chitosan					1315:1322	high deacetylated chitosan	1297:1322	high deacetylated chitosan by increasing chitosan level	1297:1351	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	10	59	theme	significant	1444:1454	arg1	interaction					1456:1466	A significant interaction	1442:1466	A significant interaction between degree of deacetylation and chitosan level	1442:1517	A significant interaction between degree of deacetylation and chitosan level was determined for serum glucose and calcium concentration.					
35726799	11	60	theme	Serum	1579:1583	arg1	content					1603:1609	Serum total antioxidant content	1579:1609	Serum total antioxidant content	1579:1609	Serum total antioxidant content increased with higher levels of dietary chitosan.					
35726799	13	61	theme	supplementation	1901:1915	arg1	levels					1882:1887	levels	1882:1887	levels of chitosan supplementation	1882:1915	However, feed conversion ratio and faeces viscosity were impaired by increasing levels of chitosan supplementation, and lightness of yolk was increased by supplementation of chitosan with a high degree of deacetylation.					
35726799	4	62	theme	egg	686:688	arg1	weight					690:695	egg weight	686:695	egg weight	686:695	Performance was assessed by recording feed intake, egg weight, daily egg production, egg quality and egg biochemistry.					
35726799	9	63	theme	chitosan	1338:1345	arg1	level					1347:1351	chitosan level	1338:1351	chitosan level	1338:1351	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	2	64	theme	Hisex	476:480	arg1	layers					488:493	140 four weeks old Hisex Brown layers	457:493	140 four weeks old Hisex Brown layers	457:493	For the experimental feeding period of 8 weeks, 140 four weeks old Hisex Brown layers were divided into 10 treatment groups, comprising 14 birds each.					
35726799	4	65	theme	daily	698:702	arg1	production					708:717	daily egg production	698:717	daily egg production	698:717	Performance was assessed by recording feed intake, egg weight, daily egg production, egg quality and egg biochemistry.					
35726799	2	66	theme	experimental	417:428	arg1	period					438:443	the experimental feeding period	413:443	the experimental feeding period of 8 weeks	413:454	For the experimental feeding period of 8 weeks, 140 four weeks old Hisex Brown layers were divided into 10 treatment groups, comprising 14 birds each.					
35726799	6	67	theme	chitosan	977:984	arg1	level					968:972	increased level	958:972	increased level of chitosan	958:984	Feed conversion ratio and faeces viscosity were deteriorated by increased level of chitosan.					
35726799	0	68	theme	laying	122:127	arg1	hens					129:132	laying hens	122:132	laying hens	122:132	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	9	69	theme	laying	1360:1365	arg1	hens					1367:1370	laying hens	1360:1370	laying hens fed low deacetylated chitosan	1360:1400	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	1	70	dep	degree	205:210	arg1	low					225:227	low	225:227	low	225:227	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	2	71	theme	8 weeks	448:454	arg1	period					438:443	the experimental feeding period	413:443	the experimental feeding period of 8 weeks	413:454	For the experimental feeding period of 8 weeks, 140 four weeks old Hisex Brown layers were divided into 10 treatment groups, comprising 14 birds each.					
35726799	8	72	theme	yolk	1156:1159	arg1	Yellowness					1138:1147	Yellowness	1138:1147	Yellowness of egg yolk	1138:1159	Yellowness of egg yolk was affected by interaction of deacetylation degree and level of chitosan.					
35726799	3	73	theme	randomised	616:625	arg1	design					627:632	a complete randomised design	605:632	a complete randomised design	605:632	The birds were housed in individual cages in a complete randomised design.					
35726799	9	74	theme	deacetylated	1380:1391	arg1	chitosan					1393:1400	low deacetylated chitosan	1376:1400	low deacetylated chitosan	1376:1400	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	4	75	theme	egg	736:738	arg1	biochemistry					740:751	egg biochemistry	736:751	egg biochemistry	736:751	Performance was assessed by recording feed intake, egg weight, daily egg production, egg quality and egg biochemistry.					
35726799	1	76	dep	low	225:227	arg1	high					238:241	high	238:241	high	238:241	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	1	76	dep	low	225:227	arg1	%					232:232	70%	230:232	70%	230:232	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	13	77	theme	faeces	1837:1842	arg1	viscosity					1844:1852	faeces viscosity	1837:1852	faeces viscosity	1837:1852	However, feed conversion ratio and faeces viscosity were impaired by increasing levels of chitosan supplementation, and lightness of yolk was increased by supplementation of chitosan with a high degree of deacetylation.					
35726799	0	78	theme	chitosan	60:67	arg1	Effects					0:6	Effects	0:6	Effects of different degrees of deacetylation	0:44	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	0	78	theme	chitosan	60:67	arg1	levels					50:55	levels	50:55	levels	50:55	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	11	79	theme	chitosan	1651:1658	arg1	levels					1633:1638	higher levels	1626:1638	higher levels of dietary chitosan	1626:1658	Serum total antioxidant content increased with higher levels of dietary chitosan.					
35726799	7	80	theme	high-degree	1058:1068	arg1	chitosan					1083:1090	high-degree deacetylated chitosan	1058:1090	high-degree deacetylated chitosan	1058:1090	Lightness of egg yolk was significantly increased in animals receiving high-degree deacetylated chitosan compared to low-degree deacetylated chitosan.					
35726799	13	81	theme	high	1992:1995	arg1	degree					1997:2002	a high degree	1990:2002	a high degree of deacetylation	1990:2019	However, feed conversion ratio and faeces viscosity were impaired by increasing levels of chitosan supplementation, and lightness of yolk was increased by supplementation of chitosan with a high degree of deacetylation.					
35726799	9	82	theme	higher	1408:1413	arg1	level					1415:1419	a higher level	1406:1419	a higher level of yolk cholesterol	1406:1439	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	2	83	theme	treatment	516:524	arg1	groups					526:531	10 treatment groups	513:531	10 treatment groups	513:531	For the experimental feeding period of 8 weeks, 140 four weeks old Hisex Brown layers were divided into 10 treatment groups, comprising 14 birds each.					
35726799	8	84	theme	deacetylation	1192:1204	arg1	degree					1206:1211	deacetylation degree	1192:1211	deacetylation degree	1192:1211	Yellowness of egg yolk was affected by interaction of deacetylation degree and level of chitosan.					
35726799	13	85	theme	feed	1811:1814	arg1	ratio					1827:1831	feed conversion ratio	1811:1831	feed conversion ratio	1811:1831	However, feed conversion ratio and faeces viscosity were impaired by increasing levels of chitosan supplementation, and lightness of yolk was increased by supplementation of chitosan with a high degree of deacetylation.					
35726799	0	86	theme	egg	85:87	arg1	traits					89:94	egg traits	85:94	egg traits	85:94	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	0	87	from	levels	50:55	arg1	performance					72:82	performance	72:82	performance	72:82	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	0	87	from	levels	50:55	arg1	traits					89:94	egg traits	85:94	egg traits	85:94	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	0	87	from	levels	50:55	arg1	biochemistry					106:117	serum biochemistry	100:117	serum biochemistry	100:117	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	9	88	theme	cholesterol	1429:1439	arg1	level					1415:1419	a higher level	1406:1419	a higher level of yolk cholesterol	1406:1439	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	6	89	theme	conversion	899:908	arg1	ratio					910:914	Feed conversion ratio	894:914	Feed conversion ratio	894:914	Feed conversion ratio and faeces viscosity were deteriorated by increased level of chitosan.					
35726799	0	90	theme	degrees	21:27	arg1	Effects					0:6	Effects	0:6	Effects of different degrees of deacetylation	0:44	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	0	90	theme	degrees	21:27	arg1	levels					50:55	levels	50:55	levels	50:55	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	12	91	theme	antioxidant	1782:1792	arg1	status					1794:1799	serum antioxidant status	1776:1799	serum antioxidant status	1776:1799	In conclusion, dietary level or different degrees of deacetylated chitosan may reduce yolk cholesterol and improve serum antioxidant status.					
35726799	2	92	dep	birds	548:552	arg1	each					554:557	each	554:557	each	554:557	For the experimental feeding period of 8 weeks, 140 four weeks old Hisex Brown layers were divided into 10 treatment groups, comprising 14 birds each.					
35726799	0	93	theme	deacetylation	32:44	arg1	degrees					21:27	different degrees	11:27	different degrees of deacetylation	11:44	Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.					
35726799	5	94	theme	viscosity	854:862	arg1	end					821:823	end	821:823	end	821:823	Serum biochemistry parameters were determined at the beginning and end of the experiment and faeces viscosity at the end of the experiment.					
35726799	5	94	theme	viscosity	854:862	arg1	beginning					807:815	beginning	807:815	beginning	807:815	Serum biochemistry parameters were determined at the beginning and end of the experiment and faeces viscosity at the end of the experiment.					
35726799	11	95	theme	total	1585:1589	arg1	content					1603:1609	Serum total antioxidant content	1579:1609	Serum total antioxidant content	1579:1609	Serum total antioxidant content increased with higher levels of dietary chitosan.					
35726799	13	96	theme	yolk	1935:1938	arg1	lightness					1922:1930	lightness	1922:1930	lightness of yolk	1922:1938	However, feed conversion ratio and faeces viscosity were impaired by increasing levels of chitosan supplementation, and lightness of yolk was increased by supplementation of chitosan with a high degree of deacetylation.					
35726799	10	97	theme	calcium	1556:1562	arg1	concentration					1564:1576	calcium concentration	1556:1576	calcium concentration	1556:1576	A significant interaction between degree of deacetylation and chitosan level was determined for serum glucose and calcium concentration.					
35726799	9	98	theme	Yolk	1236:1239	arg1	concentration					1253:1265	Yolk cholesterol concentration	1236:1265	Yolk cholesterol concentration	1236:1265	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	12	99	theme	deacetylated	1714:1725	arg1	chitosan					1727:1734	deacetylated chitosan	1714:1734	deacetylated chitosan	1714:1734	In conclusion, dietary level or different degrees of deacetylated chitosan may reduce yolk cholesterol and improve serum antioxidant status.					
35726799	6	100	theme	increased	958:966	arg1	level					968:972	increased level	958:972	increased level of chitosan	958:984	Feed conversion ratio and faeces viscosity were deteriorated by increased level of chitosan.					
35726799	9	101	contain	had	1402:1404	arg2	level					1415:1419	a higher level	1406:1419	a higher level of yolk cholesterol	1406:1439	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	9	101	contain	had	1402:1404	arg1	hens					1367:1370	laying hens	1360:1370	laying hens fed low deacetylated chitosan	1360:1400	Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol.					
35726799	7	102	theme	deacetylated	1115:1126	arg1	chitosan					1128:1135	low-degree deacetylated chitosan	1104:1135	low-degree deacetylated chitosan	1104:1135	Lightness of egg yolk was significantly increased in animals receiving high-degree deacetylated chitosan compared to low-degree deacetylated chitosan.					
35726799	1	103	theme	layers	401:406	arg1	quality					362:368	egg quality	358:368	egg quality	358:368	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	1	103	theme	layers	401:406	arg1	biochemistry					385:396	serum biochemistry	379:396	serum biochemistry	379:396	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	1	103	theme	layers	401:406	arg1	viscosity					347:355	viscosity	347:355	viscosity	347:355	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
35726799	1	103	theme	layers	401:406	arg1	egg					371:373	egg	371:373	egg	371:373	The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers.					
36215007	4	0	theme	adsorbent	781:789	arg1	ZIF-67					774:779	The SA-ME@ZIF-67 adsorbent	764:789	The SA-ME@ZIF-67 adsorbent	764:789	The SA-ME@ZIF-67 adsorbent was used to effectively adsorb Pb(II) from aqueous solutions.					
36215007	1	1	from	removal	308:314	arg1	wastewater					332:341	wastewater	332:341	wastewater	332:341	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	6	2	theme	isotherm	1066:1073	arg1	model					1075:1079	Langmuir isotherm model	1057:1079	Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively)	1057:1183	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	6	3	theme	288.15 K	1137:1144	arg1	temperatures					1121:1132	the temperatures	1117:1132	the temperatures of 288.15 K, 298.15 K, and 308.15 K	1117:1168	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	1	4	theme	chemical	263:270	arg1	grafting					272:279	chemical grafting	263:279	chemical grafting	263:279	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	2	5	theme	hydrogel	544:551	arg1	bead					553:556	the SA-ME hydrogel bead	534:556	the SA-ME hydrogel bead	534:556	Firstly, melamine (ME) was grafted onto sodium alginate (SA) by amide reaction, and then SA-ME was dropped into a solution of calcium chloride to form hydrogel bead, and ZIF-67 was grown on the SA-ME hydrogel bead by the in situ growth method.					
36215007	3	6	theme	adsorbent	605:613	arg1	ZIF-67					598:603	The SA-ME@ZIF-67 adsorbent	588:613	The SA-ME@ZIF-67 adsorbent	588:613	The SA-ME@ZIF-67 adsorbent was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction.					
36215007	1	7	theme	alginate-melamine	168:184	arg1	ZIF-67					227:232	SA-ME@ZIF-67	221:232	SA-ME@ZIF-67	221:232	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	1	7	theme	alginate-melamine	168:184	arg1	imidazolate					195:205	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67	123:218	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent	123:243	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	4	8	theme	@	773:773	arg1	ZIF-67					774:779	The SA-ME@ZIF-67 adsorbent	764:789	The SA-ME@ZIF-67 adsorbent	764:789	The SA-ME@ZIF-67 adsorbent was used to effectively adsorb Pb(II) from aqueous solutions.					
36215007	1	9	theme	zeolitic	186:193	arg1	ZIF-67					227:232	SA-ME@ZIF-67	221:232	SA-ME@ZIF-67	221:232	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	1	9	theme	zeolitic	186:193	arg1	imidazolate					195:205	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67	123:218	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent	123:243	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	6	10	theme	pseudo-second-order	1189:1207	arg1	R2 = 0.9901					1224:1234	R2 = 0.9901	1224:1234	R2 = 0.9901	1224:1234	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	6	10	theme	pseudo-second-order	1189:1207	arg1	model					1217:1221	pseudo-second-order kinetic model	1189:1221	pseudo-second-order kinetic model (R2 = 0.9901)	1189:1235	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	9	11	from	Pb	1527:1528	arg1	samples					1553:1559	real wastewater samples	1537:1559	real wastewater samples	1537:1559	In addition, the Pb(II) in real wastewater samples has been efficiently removed using the fabricated hydrogel.					
36215007	9	12	theme	real	1537:1540	arg1	samples					1553:1559	real wastewater samples	1537:1559	real wastewater samples	1537:1559	In addition, the Pb(II) in real wastewater samples has been efficiently removed using the fabricated hydrogel.					
36215007	3	13	theme	electron	686:693	arg1	microscopy					695:704	scanning electron microscopy	677:704	scanning electron microscopy	677:704	The SA-ME@ZIF-67 adsorbent was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction.					
36215007	1	14	from	wastewater	332:341	arg1	removal					308:314	the removal	304:314	the removal of lead ions in wastewater	304:341	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	1	15	theme	SA-ME	221:225	arg1	ZIF-67					227:232	SA-ME@ZIF-67	221:232	SA-ME@ZIF-67	221:232	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	1	15	theme	SA-ME	221:225	arg1	imidazolate					195:205	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67	123:218	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent	123:243	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	10	16	theme	easy	1726:1729	arg1	recyclability					1731:1743	easy recyclability	1726:1743	easy recyclability	1726:1743	The results showed that the SA-ME@ZIF-67 adsorbent had high adsorption capacity, removal efficiency, and easy recyclability for Pb(II).					
36215007	5	17	theme	lead	924:927	arg1	solution					933:940	lead ion solution	924:940	lead ion solution	924:940	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	2	18	theme	hydrogel	495:502	arg1	bead					504:507	hydrogel bead	495:507	hydrogel bead	495:507	Firstly, melamine (ME) was grafted onto sodium alginate (SA) by amide reaction, and then SA-ME was dropped into a solution of calcium chloride to form hydrogel bead, and ZIF-67 was grown on the SA-ME hydrogel bead by the in situ growth method.					
36215007	3	19	theme	photoelectron	713:725	arg1	spectroscopy					727:738	X-ray photoelectron spectroscopy	707:738	X-ray photoelectron spectroscopy	707:738	The SA-ME@ZIF-67 adsorbent was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction.					
36215007	5	20	theme	solution	933:940	arg1	time					971:974	adsorption time	960:974	adsorption time	960:974	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	5	20	theme	solution	933:940	arg1	dose					904:907	adsorbent dose	894:907	adsorbent dose	894:907	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	5	20	theme	solution	933:940	arg1	pH					918:919	initial pH	910:919	initial pH of lead ion solution	910:940	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	5	20	theme	solution	933:940	arg1	ions					888:891	lead ions	883:891	lead ions	883:891	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	5	20	theme	solution	933:940	arg1	temperature					943:953	temperature	943:953	temperature	943:953	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	10	21	theme	adsorbent	1662:1670	arg1	ZIF-67					1655:1660	the SA-ME@ZIF-67 adsorbent	1645:1670	the SA-ME@ZIF-67 adsorbent	1645:1670	The results showed that the SA-ME@ZIF-67 adsorbent had high adsorption capacity, removal efficiency, and easy recyclability for Pb(II).					
36215007	3	22	dep	Fourier	636:642	arg1	transform					644:652	transform	644:652	transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction	644:761	The SA-ME@ZIF-67 adsorbent was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction.					
36215007	10	23	theme	@	1654:1654	arg1	ZIF-67					1655:1660	the SA-ME@ZIF-67 adsorbent	1645:1670	the SA-ME@ZIF-67 adsorbent	1645:1670	The results showed that the SA-ME@ZIF-67 adsorbent had high adsorption capacity, removal efficiency, and easy recyclability for Pb(II).					
36215007	1	24	theme	low-cost	125:132	arg1	ZIF-67					227:232	SA-ME@ZIF-67	221:232	SA-ME@ZIF-67	221:232	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	1	24	theme	low-cost	125:132	arg1	imidazolate					195:205	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67	123:218	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent	123:243	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	7	25	from	308.15 K	1286:1293	arg1	model					1277:1281	the Langmuir model	1264:1281	the Langmuir model at 308.15 K	1264:1293	According to the Langmuir model at 308.15 K, the maximum adsorption capacity of the adsorbent for Pb(II) was 634.99 mg/g.					
36215007	3	26	theme	X-ray	745:749	arg1	diffraction					751:761	X-ray diffraction	745:761	X-ray diffraction	745:761	The SA-ME@ZIF-67 adsorbent was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction.					
36215007	0	27	theme	adsorption	74:83	arg1	application					85:95	its adsorption application	70:95	its adsorption application for Pb(II)	70:106	Fabrication of sodium alginate-melamine@ZIF-67 composite hydrogel and its adsorption application for Pb(II) in wastewater.					
36215007	1	28	theme	ions	324:327	arg1	removal					308:314	the removal	304:314	the removal of lead ions in wastewater	304:341	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	1	29	theme	environmental-friendly	138:159	arg1	ZIF-67					227:232	SA-ME@ZIF-67	221:232	SA-ME@ZIF-67	221:232	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	1	29	theme	environmental-friendly	138:159	arg1	imidazolate					195:205	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67	123:218	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent	123:243	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	2	30	dep	in	565:566	arg1	situ					568:571	situ	568:571	situ	568:571	Firstly, melamine (ME) was grafted onto sodium alginate (SA) by amide reaction, and then SA-ME was dropped into a solution of calcium chloride to form hydrogel bead, and ZIF-67 was grown on the SA-ME hydrogel bead by the in situ growth method.					
36215007	0	31	from	Fabrication	0:10	arg1	wastewater					111:120	wastewater	111:120	wastewater	111:120	Fabrication of sodium alginate-melamine@ZIF-67 composite hydrogel and its adsorption application for Pb(II) in wastewater.					
36215007	7	32	theme	maximum	1300:1306	arg1	634.99 mg/g					1360:1370	634.99 mg/g	1360:1370	634.99 mg/g	1360:1370	According to the Langmuir model at 308.15 K, the maximum adsorption capacity of the adsorbent for Pb(II) was 634.99 mg/g.					
36215007	7	32	theme	maximum	1300:1306	arg1	capacity					1319:1326	the maximum adsorption capacity	1296:1326	the maximum adsorption capacity of the adsorbent for Pb(II)	1296:1354	According to the Langmuir model at 308.15 K, the maximum adsorption capacity of the adsorbent for Pb(II) was 634.99 mg/g.					
36215007	6	33	from	temperatures	1121:1132	arg1	0.9623					1107:1112	0.9623	1107:1112	0.9623	1107:1112	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	0	34	theme	ZIF-67	40:45	arg1	hydrogel					57:64	sodium alginate-melamine@ZIF-67 composite hydrogel	15:64	sodium alginate-melamine@ZIF-67 composite hydrogel	15:64	Fabrication of sodium alginate-melamine@ZIF-67 composite hydrogel and its adsorption application for Pb(II) in wastewater.					
36215007	5	35	theme	lead	883:886	arg1	ions					888:891	lead ions	883:891	lead ions	883:891	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	5	35	theme	lead	883:886	arg1	dose					904:907	adsorbent dose	894:907	adsorbent dose	894:907	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	5	35	theme	lead	883:886	arg1	pH					918:919	initial pH	910:919	initial pH of lead ion solution	910:940	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	5	35	theme	lead	883:886	arg1	time					971:974	adsorption time	960:974	adsorption time	960:974	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	5	35	theme	lead	883:886	arg1	temperature					943:953	temperature	943:953	temperature	943:953	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	8	36	theme	recycling	1377:1385	arg1	possible					1420:1427	possible	1420:1427	possible	1420:1427	The recycling application of the adsorbent was possible as it was easily collected and reused after five adsorption-regeneration cycles.					
36215007	8	36	theme	recycling	1377:1385	arg1	application					1387:1397	The recycling application	1373:1397	The recycling application of the adsorbent	1373:1414	The recycling application of the adsorbent was possible as it was easily collected and reused after five adsorption-regeneration cycles.					
36215007	2	37	theme	sodium	384:389	arg1	SA					401:402	SA	401:402	SA	401:402	Firstly, melamine (ME) was grafted onto sodium alginate (SA) by amide reaction, and then SA-ME was dropped into a solution of calcium chloride to form hydrogel bead, and ZIF-67 was grown on the SA-ME hydrogel bead by the in situ growth method.					
36215007	2	37	theme	sodium	384:389	arg1	alginate					391:398	sodium alginate	384:398	sodium alginate (SA)	384:403	Firstly, melamine (ME) was grafted onto sodium alginate (SA) by amide reaction, and then SA-ME was dropped into a solution of calcium chloride to form hydrogel bead, and ZIF-67 was grown on the SA-ME hydrogel bead by the in situ growth method.					
36215007	0	38	theme	sodium	15:20	arg1	hydrogel					57:64	sodium alginate-melamine@ZIF-67 composite hydrogel	15:64	sodium alginate-melamine@ZIF-67 composite hydrogel	15:64	Fabrication of sodium alginate-melamine@ZIF-67 composite hydrogel and its adsorption application for Pb(II) in wastewater.					
36215007	1	39	theme	in	285:286	arg1	growth					293:298	in situ growth	285:298	in situ growth	285:298	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	8	40	theme	adsorbent	1406:1414	arg1	possible					1420:1427	possible	1420:1427	possible	1420:1427	The recycling application of the adsorbent was possible as it was easily collected and reused after five adsorption-regeneration cycles.					
36215007	8	40	theme	adsorbent	1406:1414	arg1	application					1387:1397	The recycling application	1373:1397	The recycling application of the adsorbent	1373:1414	The recycling application of the adsorbent was possible as it was easily collected and reused after five adsorption-regeneration cycles.					
36215007	0	41	theme	@	39:39	arg1	hydrogel					57:64	sodium alginate-melamine@ZIF-67 composite hydrogel	15:64	sodium alginate-melamine@ZIF-67 composite hydrogel	15:64	Fabrication of sodium alginate-melamine@ZIF-67 composite hydrogel and its adsorption application for Pb(II) in wastewater.					
36215007	5	42	theme	initial	910:916	arg1	ions					888:891	lead ions	883:891	lead ions	883:891	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	5	42	theme	initial	910:916	arg1	pH					918:919	initial pH	910:919	initial pH of lead ion solution	910:940	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	2	43	theme	amide	408:412	arg1	reaction					414:421	amide reaction	408:421	amide reaction	408:421	Firstly, melamine (ME) was grafted onto sodium alginate (SA) by amide reaction, and then SA-ME was dropped into a solution of calcium chloride to form hydrogel bead, and ZIF-67 was grown on the SA-ME hydrogel bead by the in situ growth method.					
36215007	10	44	theme	high	1676:1679	arg1	capacity					1692:1699	high adsorption capacity	1676:1699	high adsorption capacity	1676:1699	The results showed that the SA-ME@ZIF-67 adsorbent had high adsorption capacity, removal efficiency, and easy recyclability for Pb(II).					
36215007	0	45	theme	composite	47:55	arg1	hydrogel					57:64	sodium alginate-melamine@ZIF-67 composite hydrogel	15:64	sodium alginate-melamine@ZIF-67 composite hydrogel	15:64	Fabrication of sodium alginate-melamine@ZIF-67 composite hydrogel and its adsorption application for Pb(II) in wastewater.					
36215007	1	46	theme	@	226:226	arg1	ZIF-67					227:232	SA-ME@ZIF-67	221:232	SA-ME@ZIF-67	221:232	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	1	46	theme	@	226:226	arg1	imidazolate					195:205	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67	123:218	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent	123:243	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	10	47	theme	removal	1702:1708	arg1	efficiency					1710:1719	removal efficiency	1702:1719	removal efficiency	1702:1719	The results showed that the SA-ME@ZIF-67 adsorbent had high adsorption capacity, removal efficiency, and easy recyclability for Pb(II).					
36215007	3	48	dep	transform	644:652	arg1	infrared					654:661	infrared	654:661	transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction	644:761	The SA-ME@ZIF-67 adsorbent was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction.					
36215007	7	49	theme	adsorbent	1335:1343	arg1	634.99 mg/g					1360:1370	634.99 mg/g	1360:1370	634.99 mg/g	1360:1370	According to the Langmuir model at 308.15 K, the maximum adsorption capacity of the adsorbent for Pb(II) was 634.99 mg/g.					
36215007	7	49	theme	adsorbent	1335:1343	arg1	capacity					1319:1326	the maximum adsorption capacity	1296:1326	the maximum adsorption capacity of the adsorbent for Pb(II)	1296:1354	According to the Langmuir model at 308.15 K, the maximum adsorption capacity of the adsorbent for Pb(II) was 634.99 mg/g.					
36215007	3	50	theme	SA-ME	592:596	arg1	ZIF-67					598:603	The SA-ME@ZIF-67 adsorbent	588:613	The SA-ME@ZIF-67 adsorbent	588:613	The SA-ME@ZIF-67 adsorbent was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction.					
36215007	6	51	theme	Langmuir	1057:1064	arg1	model					1075:1079	Langmuir isotherm model	1057:1079	Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively)	1057:1183	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	8	52	theme	adsorption-regeneration	1478:1500	arg1	cycles					1502:1507	five adsorption-regeneration cycles	1473:1507	five adsorption-regeneration cycles	1473:1507	The recycling application of the adsorbent was possible as it was easily collected and reused after five adsorption-regeneration cycles.					
36215007	1	53	theme	sodium	161:166	arg1	ZIF-67					227:232	SA-ME@ZIF-67	221:232	SA-ME@ZIF-67	221:232	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	1	53	theme	sodium	161:166	arg1	imidazolate					195:205	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67	123:218	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent	123:243	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	7	54	theme	adsorption	1308:1317	arg1	634.99 mg/g					1360:1370	634.99 mg/g	1360:1370	634.99 mg/g	1360:1370	According to the Langmuir model at 308.15 K, the maximum adsorption capacity of the adsorbent for Pb(II) was 634.99 mg/g.					
36215007	7	54	theme	adsorption	1308:1317	arg1	capacity					1319:1326	the maximum adsorption capacity	1296:1326	the maximum adsorption capacity of the adsorbent for Pb(II)	1296:1354	According to the Langmuir model at 308.15 K, the maximum adsorption capacity of the adsorbent for Pb(II) was 634.99 mg/g.					
36215007	2	55	theme	SA-ME	538:542	arg1	bead					553:556	the SA-ME hydrogel bead	534:556	the SA-ME hydrogel bead	534:556	Firstly, melamine (ME) was grafted onto sodium alginate (SA) by amide reaction, and then SA-ME was dropped into a solution of calcium chloride to form hydrogel bead, and ZIF-67 was grown on the SA-ME hydrogel bead by the in situ growth method.					
36215007	1	56	theme	@	185:185	arg1	ZIF-67					227:232	SA-ME@ZIF-67	221:232	SA-ME@ZIF-67	221:232	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	1	56	theme	@	185:185	arg1	imidazolate					195:205	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67	123:218	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent	123:243	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	4	57	theme	SA-ME	768:772	arg1	ZIF-67					774:779	The SA-ME@ZIF-67 adsorbent	764:789	The SA-ME@ZIF-67 adsorbent	764:789	The SA-ME@ZIF-67 adsorbent was used to effectively adsorb Pb(II) from aqueous solutions.					
36215007	6	58	theme	kinetic	1209:1215	arg1	R2 = 0.9901					1224:1234	R2 = 0.9901	1224:1234	R2 = 0.9901	1224:1234	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	6	58	theme	kinetic	1209:1215	arg1	model					1217:1221	pseudo-second-order kinetic model	1189:1221	pseudo-second-order kinetic model (R2 = 0.9901)	1189:1235	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	2	59	theme	chloride	478:485	arg1	solution					458:465	a solution	456:465	a solution of calcium chloride	456:485	Firstly, melamine (ME) was grafted onto sodium alginate (SA) by amide reaction, and then SA-ME was dropped into a solution of calcium chloride to form hydrogel bead, and ZIF-67 was grown on the SA-ME hydrogel bead by the in situ growth method.					
36215007	7	60	theme	Langmuir	1268:1275	arg1	model					1277:1281	the Langmuir model	1264:1281	the Langmuir model at 308.15 K	1264:1293	According to the Langmuir model at 308.15 K, the maximum adsorption capacity of the adsorbent for Pb(II) was 634.99 mg/g.					
36215007	10	61	theme	SA-ME	1649:1653	arg1	ZIF-67					1655:1660	the SA-ME@ZIF-67 adsorbent	1645:1670	the SA-ME@ZIF-67 adsorbent	1645:1670	The results showed that the SA-ME@ZIF-67 adsorbent had high adsorption capacity, removal efficiency, and easy recyclability for Pb(II).					
36215007	9	62	theme	wastewater	1542:1551	arg1	samples					1553:1559	real wastewater samples	1537:1559	real wastewater samples	1537:1559	In addition, the Pb(II) in real wastewater samples has been efficiently removed using the fabricated hydrogel.					
36215007	5	63	theme	adsorption	960:969	arg1	time					971:974	adsorption time	960:974	adsorption time	960:974	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	5	63	theme	adsorption	960:969	arg1	ions					888:891	lead ions	883:891	lead ions	883:891	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	6	64	dep	model	1075:1079	arg1	R2 = 0.9281					1082:1092	R2 = 0.9281	1082:1092	R2 = 0.9281	1082:1092	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	6	64	dep	model	1075:1079	arg1	0.9420					1095:1100	0.9420	1095:1100	0.9420	1095:1100	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	6	64	dep	model	1075:1079	arg1	0.9623					1107:1112	0.9623	1107:1112	0.9623	1107:1112	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	3	65	theme	scanning	677:684	arg1	microscopy					695:704	scanning electron microscopy	677:704	scanning electron microscopy	677:704	The SA-ME@ZIF-67 adsorbent was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction.					
36215007	6	66	theme	308.15 K	1161:1168	arg1	temperatures					1121:1132	the temperatures	1117:1132	the temperatures of 288.15 K, 298.15 K, and 308.15 K	1117:1168	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	5	67	theme	ion	929:931	arg1	solution					933:940	lead ion solution	924:940	lead ion solution	924:940	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	1	68	dep	in	285:286	arg1	situ					288:291	situ	288:291	situ	288:291	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	3	69	theme	X-ray	707:711	arg1	spectroscopy					727:738	X-ray photoelectron spectroscopy	707:738	X-ray photoelectron spectroscopy	707:738	The SA-ME@ZIF-67 adsorbent was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction.					
36215007	0	70	theme	application	85:95	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of sodium alginate-melamine@ZIF-67 composite hydrogel and its adsorption application for Pb(II) in wastewater.	0:121	Fabrication of sodium alginate-melamine@ZIF-67 composite hydrogel and its adsorption application for Pb(II) in wastewater.					
36215007	1	71	theme	lead	319:322	arg1	ions					324:327	lead ions	319:327	lead ions in wastewater	319:341	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	9	72	theme	fabricated	1600:1609	arg1	hydrogel					1611:1618	the fabricated hydrogel	1596:1618	the fabricated hydrogel	1596:1618	In addition, the Pb(II) in real wastewater samples has been efficiently removed using the fabricated hydrogel.					
36215007	2	73	theme	in	565:566	arg1	method					580:585	the in situ growth method	561:585	the in situ growth method	561:585	Firstly, melamine (ME) was grafted onto sodium alginate (SA) by amide reaction, and then SA-ME was dropped into a solution of calcium chloride to form hydrogel bead, and ZIF-67 was grown on the SA-ME hydrogel bead by the in situ growth method.					
36215007	5	74	theme	initial	857:863	arg1	concentrations					865:878	The initial concentrations	853:878	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material	853:991	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	6	75	theme	adsorption	1013:1022	arg1	isotherms					1024:1032	The adsorption isotherms	1009:1032	The adsorption isotherms	1009:1032	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	4	76	theme	aqueous	834:840	arg1	solutions					842:850	aqueous solutions	834:850	aqueous solutions	834:850	The SA-ME@ZIF-67 adsorbent was used to effectively adsorb Pb(II) from aqueous solutions.					
36215007	2	77	theme	calcium	470:476	arg1	chloride					478:485	calcium chloride	470:485	calcium chloride	470:485	Firstly, melamine (ME) was grafted onto sodium alginate (SA) by amide reaction, and then SA-ME was dropped into a solution of calcium chloride to form hydrogel bead, and ZIF-67 was grown on the SA-ME hydrogel bead by the in situ growth method.					
36215007	2	78	theme	growth	573:578	arg1	method					580:585	the in situ growth method	561:585	the in situ growth method	561:585	Firstly, melamine (ME) was grafted onto sodium alginate (SA) by amide reaction, and then SA-ME was dropped into a solution of calcium chloride to form hydrogel bead, and ZIF-67 was grown on the SA-ME hydrogel bead by the in situ growth method.					
36215007	0	79	theme	alginate-melamine	22:38	arg1	hydrogel					57:64	sodium alginate-melamine@ZIF-67 composite hydrogel	15:64	sodium alginate-melamine@ZIF-67 composite hydrogel	15:64	Fabrication of sodium alginate-melamine@ZIF-67 composite hydrogel and its adsorption application for Pb(II) in wastewater.					
36215007	4	80	used	used	795:798	arg2	ZIF-67					774:779	The SA-ME@ZIF-67 adsorbent	764:789	The SA-ME@ZIF-67 adsorbent	764:789	The SA-ME@ZIF-67 adsorbent was used to effectively adsorb Pb(II) from aqueous solutions.					
36215007	1	81	from	ions	324:327	arg1	wastewater					332:341	wastewater	332:341	wastewater	332:341	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	10	82	theme	adsorption	1681:1690	arg1	capacity					1692:1699	high adsorption capacity	1676:1699	high adsorption capacity	1676:1699	The results showed that the SA-ME@ZIF-67 adsorbent had high adsorption capacity, removal efficiency, and easy recyclability for Pb(II).					
36215007	0	83	theme	hydrogel	57:64	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of sodium alginate-melamine@ZIF-67 composite hydrogel and its adsorption application for Pb(II) in wastewater.	0:121	Fabrication of sodium alginate-melamine@ZIF-67 composite hydrogel and its adsorption application for Pb(II) in wastewater.					
36215007	10	84	contain	had	1672:1674	arg1	ZIF-67					1655:1660	the SA-ME@ZIF-67 adsorbent	1645:1670	the SA-ME@ZIF-67 adsorbent	1645:1670	The results showed that the SA-ME@ZIF-67 adsorbent had high adsorption capacity, removal efficiency, and easy recyclability for Pb(II).					
36215007	10	84	contain	had	1672:1674	arg2	efficiency					1710:1719	removal efficiency	1702:1719	removal efficiency	1702:1719	The results showed that the SA-ME@ZIF-67 adsorbent had high adsorption capacity, removal efficiency, and easy recyclability for Pb(II).					
36215007	10	84	contain	had	1672:1674	arg2	recyclability					1731:1743	easy recyclability	1726:1743	easy recyclability	1726:1743	The results showed that the SA-ME@ZIF-67 adsorbent had high adsorption capacity, removal efficiency, and easy recyclability for Pb(II).					
36215007	10	84	contain	had	1672:1674	arg2	capacity					1692:1699	high adsorption capacity	1676:1699	high adsorption capacity	1676:1699	The results showed that the SA-ME@ZIF-67 adsorbent had high adsorption capacity, removal efficiency, and easy recyclability for Pb(II).					
36215007	6	85	theme	298.15 K	1147:1154	arg1	temperatures					1121:1132	the temperatures	1117:1132	the temperatures of 288.15 K, 298.15 K, and 308.15 K	1117:1168	The adsorption isotherms and kinetics fitted to Langmuir isotherm model (R2 = 0.9281, 0.9420, and 0.9623 at the temperatures of 288.15 K, 298.15 K, and 308.15 K, respectively) and pseudo-second-order kinetic model (R2 = 0.9901) respectively.					
36215007	5	86	theme	ions	888:891	arg1	concentrations					865:878	The initial concentrations	853:878	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material	853:991	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	5	87	theme	adsorbent	894:902	arg1	ions					888:891	lead ions	883:891	lead ions	883:891	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	5	87	theme	adsorbent	894:902	arg1	dose					904:907	adsorbent dose	894:907	adsorbent dose	894:907	The initial concentrations of lead ions, adsorbent dose, initial pH of lead ion solution, temperature, and adsorption time for the material were optimized.					
36215007	3	88	theme	@	597:597	arg1	ZIF-67					598:603	The SA-ME@ZIF-67 adsorbent	588:613	The SA-ME@ZIF-67 adsorbent	588:613	The SA-ME@ZIF-67 adsorbent was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction.					
36215007	1	89	theme	adsorbent	235:243	arg1	ZIF-67					227:232	SA-ME@ZIF-67	221:232	SA-ME@ZIF-67	221:232	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
36215007	1	89	theme	adsorbent	235:243	arg1	imidazolate					195:205	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67	123:218	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent	123:243	A low-cost and environmental-friendly sodium alginate-melamine@zeolitic imidazolate framework-67 (SA-ME@ZIF-67) adsorbent was fabricated by chemical grafting and in situ growth for the removal of lead ions in wastewater.					
35916402	2	0	from	transitions	411:421	arg1	species					441:447	many bacterial species	426:447	many bacterial species	426:447	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	7	1	theme	proper	1276:1281	arg1	regulation					1292:1301	proper c-di-GMP regulation	1276:1301	proper c-di-GMP regulation during beneficial animal colonization	1276:1339	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	8	2	theme	biofilm	1701:1707	arg1	formation					1709:1717	biofilm formation	1701:1717	biofilm formation	1701:1717	IMPORTANCE There is substantial interest in studying cyclic diguanylate (c-di-GMP) in pathogenic and environmental bacteria, which has led to an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility.					
35916402	6	3	theme	other	1214:1218	arg1	polysaccharide					1220:1233	the other polysaccharide	1210:1233	the other polysaccharide	1210:1233	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	11	4	theme	animal	2115:2120	arg1	host					2122:2125	the animal host	2111:2125	the animal host	2111:2125	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	10	5	from	bacterium	1979:1987	arg1	advantage					1935:1943	advantage	1935:1943	advantage of recent genetic advances in the bacterium	1935:1987	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	4	6	theme	high	663:666	arg1	levels					668:673	either low or high levels	649:673	either low or high levels of c-di-GMP	649:685	We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired.					
35916402	11	7	from	studies	2280:2286	arg1	interaction					2165:2175	a c-di-GMP-dependent genetic interaction	2136:2175	a c-di-GMP-dependent genetic interaction	2136:2175	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	11	7	from	studies	2280:2286	arg1	evident					2255:2261	evident	2255:2261	evident	2255:2261	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	5	8	theme	phosphodiesterase	868:884	arg1	Expression					830:839	Expression	830:839	Expression of a heterologous c-di-GMP phosphodiesterase	830:884	Expression of a heterologous c-di-GMP phosphodiesterase restored colonization, demonstrating that the effect is due to high c-di-GMP levels.					
35916402	9	9	theme	less	1762:1765	arg1	focus					1767:1771	considerably less focus	1749:1771	considerably less focus	1749:1771	However, considerably less focus has been placed on understanding how this compound contributes to beneficial colonization.					
35916402	1	10	from	state	274:278	arg1	mucus					288:292	host mucus	283:292	host mucus	283:292	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	3	11	theme	host	573:576	arg1	colonization					578:589	host colonization	573:589	host colonization	573:589	V. fischeri encodes 50 proteins predicted to synthesize and/or degrade c-di-GMP, but a role for c-di-GMP regulation during host colonization has not been investigated.					
35916402	6	12	theme	constitutive	978:989	arg1	state					1005:1009	the constitutive high-c-di-GMP state	974:1009	the constitutive high-c-di-GMP state	974:1009	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	7	13	theme	exopolysaccharide	1443:1459	arg1	systems					1461:1467	multiple exopolysaccharide systems	1434:1467	multiple exopolysaccharide systems in host-associated aggregates	1434:1497	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	11	14	theme	cellulose	2417:2425	arg1	synthesis					2427:2435	increased cellulose synthesis	2407:2435	increased cellulose synthesis	2407:2435	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	10	15	theme	c-di-GMP	2052:2059	arg1	phenotypes					2061:2070	track c-di-GMP phenotypes	2046:2070	track c-di-GMP phenotypes	2046:2070	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	6	16	theme	regulatory	1107:1116	arg1	interaction					1118:1128	a regulatory interaction	1105:1128	a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide	1105:1233	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	1	17	theme	squid	128:132	arg1	colonization					91:102	colonization	91:102	colonization of the Hawaiian bobtail squid (Euprymna scolopes)	91:152	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	11	18	theme	genetic	2157:2163	arg1	interaction					2165:2175	a c-di-GMP-dependent genetic interaction	2136:2175	a c-di-GMP-dependent genetic interaction	2136:2175	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	11	18	theme	genetic	2157:2163	arg1	evident					2255:2261	evident	2255:2261	evident	2255:2261	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	4	19	theme	low	656:658	arg1	levels					668:673	either low or high levels	649:673	either low or high levels of c-di-GMP	649:685	We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired.					
35916402	10	20	theme	squid	1907:1911	arg1	system					1919:1924	the Vibrio fischeri-Hawaiian bobtail squid study system	1870:1924	the Vibrio fischeri-Hawaiian bobtail squid study system	1870:1924	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	5	21	theme	heterologous	846:857	arg1	phosphodiesterase					868:884	a heterologous c-di-GMP phosphodiesterase	844:884	a heterologous c-di-GMP phosphodiesterase	844:884	Expression of a heterologous c-di-GMP phosphodiesterase restored colonization, demonstrating that the effect is due to high c-di-GMP levels.					
35916402	10	22	theme	symbiotic	2077:2085	arg1	interaction					2087:2097	a symbiotic interaction	2075:2097	a symbiotic interaction	2075:2097	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	10	23	theme	fischeri-Hawaiian	1881:1897	arg1	system					1919:1924	the Vibrio fischeri-Hawaiian bobtail squid study system	1870:1924	the Vibrio fischeri-Hawaiian bobtail squid study system	1870:1924	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	8	24	dep	IMPORTANCE	1500:1509	arg1	There					1511:1515	There	1511:1515	There	1511:1515	IMPORTANCE There is substantial interest in studying cyclic diguanylate (c-di-GMP) in pathogenic and environmental bacteria, which has led to an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility.					
35916402	11	25	theme	distinct	2189:2196	arg1	cellulose					2231:2239	cellulose	2231:2239	cellulose	2231:2239	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	11	25	theme	distinct	2189:2196	arg1	Syp					2223:2225	Syp	2223:2225	Syp	2223:2225	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	11	25	theme	distinct	2189:2196	arg1	polysaccharides					2206:2220	two distinct biofilm polysaccharides	2185:2220	two distinct biofilm polysaccharides	2185:2220	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	1	26	theme	Euprymna	135:142	arg1	squid					128:132	the Hawaiian bobtail squid	107:132	the Hawaiian bobtail squid (Euprymna scolopes)	107:152	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	1	26	theme	Euprymna	135:142	arg1	scolopes					144:151	Euprymna scolopes	135:151	Euprymna scolopes	135:151	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	6	27	theme	reduced	1045:1051	arg1	motility					1053:1060	reduced motility	1045:1060	reduced motility	1045:1060	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	0	28	theme	Bacterial	60:68	arg1	Colonization					70:81	Beneficial Bacterial Colonization	49:81	Beneficial Bacterial Colonization	49:81	High Levels of Cyclic Diguanylate Interfere with Beneficial Bacterial Colonization.					
35916402	11	29	theme	culture-based	2266:2278	arg1	studies					2280:2286	culture-based studies	2266:2286	culture-based studies	2266:2286	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	7	30	from	aggregates	1488:1497	arg1	interplay					1421:1429	an interplay	1418:1429	an interplay of multiple exopolysaccharide systems in host-associated aggregates	1418:1497	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	9	31	theme	beneficial	1839:1848	arg1	colonization					1850:1861	beneficial colonization	1839:1861	beneficial colonization	1839:1861	However, considerably less focus has been placed on understanding how this compound contributes to beneficial colonization.					
35916402	1	32	theme	Vibrio	155:160	arg1	fischeri					162:169	Vibrio fischeri	155:169	Vibrio fischeri bacteria	155:178	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	6	33	theme	colonizing	1012:1021	arg1	fischeri					1026:1033	colonizing V. fischeri	1012:1033	colonizing V. fischeri	1012:1033	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	4	34	theme	colonization	763:774	arg1	defect					776:781	no colonization defect	760:781	no colonization defect	760:781	We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired.					
35916402	8	35	from	diguanylate	1560:1570	arg1	bacteria					1615:1622	pathogenic and environmental bacteria	1586:1622	pathogenic and environmental bacteria	1586:1622	IMPORTANCE There is substantial interest in studying cyclic diguanylate (c-di-GMP) in pathogenic and environmental bacteria, which has led to an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility.					
35916402	4	36	theme	low-c-di-GMP	736:747	arg1	strain					749:754	a low-c-di-GMP strain	734:754	a low-c-di-GMP strain	734:754	We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired.					
35916402	0	37	theme	High	0:3	arg1	Levels					5:10	High Levels	0:10	High Levels of Cyclic Diguanylate	0:32	High Levels of Cyclic Diguanylate Interfere with Beneficial Bacterial Colonization.					
35916402	1	38	theme	biofilm	266:272	arg1	state					274:278	a biofilm state	264:278	a biofilm state in host mucus	264:292	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	5	39	theme	c-di-GMP	954:961	arg1	levels					963:968	high c-di-GMP levels	949:968	high c-di-GMP levels	949:968	Expression of a heterologous c-di-GMP phosphodiesterase restored colonization, demonstrating that the effect is due to high c-di-GMP levels.					
35916402	10	40	theme	recent	1948:1953	arg1	advances					1963:1970	recent genetic advances	1948:1970	recent genetic advances in the bacterium	1948:1987	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	2	41	theme	bacterial	431:439	arg1	species					441:447	many bacterial species	426:447	many bacterial species	426:447	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	11	42	dep	composition	2319:2329	arg1	the					2315:2317	the	2315:2317	the	2315:2317	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	1	43	theme	Hawaiian	111:118	arg1	squid					128:132	the Hawaiian bobtail squid	107:132	the Hawaiian bobtail squid (Euprymna scolopes)	107:152	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	1	43	theme	Hawaiian	111:118	arg1	scolopes					144:151	Euprymna scolopes	135:151	Euprymna scolopes	135:151	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	6	44	theme	biofilm	1071:1077	arg1	morphology					1089:1098	altered biofilm aggregate morphology	1063:1098	altered biofilm aggregate morphology	1063:1098	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	12	45	theme	squid	2614:2618	arg1	host					2620:2623	the squid host	2610:2623	the squid host	2610:2623	This study reveals important parallels between pathogenic and beneficial colonization and additionally identifies c-di-GMP-dependent regulation that occurs specifically in the squid host.					
35916402	2	46	theme	motility-biofilm	394:409	arg1	transitions					411:421	motility-biofilm transitions	394:421	motility-biofilm transitions in many bacterial species	394:447	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	3	47	theme	V.	450:451	arg1	fischeri					453:460	V. fischeri	450:460	V. fischeri	450:460	V. fischeri encodes 50 proteins predicted to synthesize and/or degrade c-di-GMP, but a role for c-di-GMP regulation during host colonization has not been investigated.					
35916402	3	47	theme	V.	450:451	arg1	proteins					473:480	50 proteins	470:480	50 proteins predicted to synthesize and/or degrade c-di-GMP	470:528	V. fischeri encodes 50 proteins predicted to synthesize and/or degrade c-di-GMP, but a role for c-di-GMP regulation during host colonization has not been investigated.					
35916402	5	48	theme	c-di-GMP	859:866	arg1	phosphodiesterase					868:884	a heterologous c-di-GMP phosphodiesterase	844:884	a heterologous c-di-GMP phosphodiesterase	844:884	Expression of a heterologous c-di-GMP phosphodiesterase restored colonization, demonstrating that the effect is due to high c-di-GMP levels.					
35916402	0	49	theme	Diguanylate	22:32	arg1	Levels					5:10	High Levels	0:10	High Levels of Cyclic Diguanylate	0:32	High Levels of Cyclic Diguanylate Interfere with Beneficial Bacterial Colonization.					
35916402	7	50	theme	multiple	1353:1360	arg1	pathways					1362:1369	multiple pathways	1353:1369	multiple pathways regulated by c-di-GMP in the host	1353:1403	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	11	51	theme	elevated	2289:2296	arg1	c-di-GMP					2298:2305	elevated c-di-GMP	2289:2305	elevated c-di-GMP	2289:2305	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	6	52	theme	polysaccharide	1157:1170	arg1	locus					1172:1176	one polysaccharide locus	1153:1176	one polysaccharide locus	1153:1176	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	4	53	theme	high	786:789	arg1	strain					800:805	a high c-di-GMP strain	784:805	a high c-di-GMP strain	784:805	We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired.					
35916402	4	53	theme	high	786:789	arg1	impaired					820:827	impaired	820:827	impaired	820:827	We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired.					
35916402	1	54	theme	planktonic	218:227	arg1	state					236:240	a planktonic motile state	216:240	a planktonic motile state in the environment	216:259	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	7	55	theme	animal	1321:1326	arg1	colonization					1328:1339	beneficial animal colonization	1310:1339	beneficial animal colonization	1310:1339	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	10	56	theme	advances	1963:1970	arg1	advantage					1935:1943	advantage	1935:1943	advantage of recent genetic advances in the bacterium	1935:1987	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	10	57	from	advantage	1935:1943	arg1	bacterium					1979:1987	the bacterium	1975:1987	the bacterium	1975:1987	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	11	58	from	Studies	2100:2106	arg1	host					2122:2125	the animal host	2111:2125	the animal host	2111:2125	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	8	59	theme	c-di-GMP	1677:1684	arg1	levels					1686:1691	high c-di-GMP levels	1672:1691	high c-di-GMP levels	1672:1691	IMPORTANCE There is substantial interest in studying cyclic diguanylate (c-di-GMP) in pathogenic and environmental bacteria, which has led to an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility.					
35916402	10	60	theme	c-di-GMP	2001:2008	arg1	levels					2010:2015	c-di-GMP levels	2001:2015	c-di-GMP levels	2001:2015	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	2	61	theme	cytoplasmic	330:340	arg1	important					369:377	important	369:377	important	369:377	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	2	61	theme	cytoplasmic	330:340	arg1	diguanylate					302:312	Cyclic diguanylate	295:312	Cyclic diguanylate (c-di-GMP)	295:323	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	2	61	theme	cytoplasmic	330:340	arg1	molecule					352:359	a cytoplasmic signaling molecule	328:359	a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species	328:447	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	7	62	theme	c-di-GMP	1283:1290	arg1	regulation					1292:1301	proper c-di-GMP regulation	1276:1301	proper c-di-GMP regulation during beneficial animal colonization	1276:1339	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	12	63	theme	pathogenic	2485:2494	arg1	colonization					2511:2522	pathogenic and beneficial colonization	2485:2522	pathogenic and beneficial colonization	2485:2522	This study reveals important parallels between pathogenic and beneficial colonization and additionally identifies c-di-GMP-dependent regulation that occurs specifically in the squid host.					
35916402	10	64	from	advances	1963:1970	arg1	bacterium					1979:1987	the bacterium	1975:1987	the bacterium	1975:1987	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	11	65	theme	syp	2382:2384	arg1	transcription					2386:2398	syp transcription	2382:2398	syp transcription	2382:2398	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	12	66	theme	beneficial	2500:2509	arg1	colonization					2511:2522	pathogenic and beneficial colonization	2485:2522	pathogenic and beneficial colonization	2485:2522	This study reveals important parallels between pathogenic and beneficial colonization and additionally identifies c-di-GMP-dependent regulation that occurs specifically in the squid host.					
35916402	11	67	theme	c-di-GMP-dependent	2138:2155	arg1	interaction					2165:2175	a c-di-GMP-dependent genetic interaction	2136:2175	a c-di-GMP-dependent genetic interaction	2136:2175	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	11	67	theme	c-di-GMP-dependent	2138:2155	arg1	evident					2255:2261	evident	2255:2261	evident	2255:2261	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	1	68	theme	fischeri	162:169	arg1	bacteria					171:178	Vibrio fischeri bacteria	155:178	Vibrio fischeri bacteria	155:178	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	10	69	theme	Vibrio	1874:1879	arg1	system					1919:1924	the Vibrio fischeri-Hawaiian bobtail squid study system	1870:1924	the Vibrio fischeri-Hawaiian bobtail squid study system	1870:1924	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	6	70	theme	high-c-di-GMP	991:1003	arg1	state					1005:1009	the constitutive high-c-di-GMP state	974:1009	the constitutive high-c-di-GMP state	974:1009	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	7	71	theme	host-associated	1472:1486	arg1	aggregates					1488:1497	host-associated aggregates	1472:1497	host-associated aggregates	1472:1497	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	11	72	theme	increased	2407:2415	arg1	synthesis					2427:2435	increased cellulose synthesis	2407:2435	increased cellulose synthesis	2407:2435	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	4	73	contain	had	756:758	arg1	strain					749:754	a low-c-di-GMP strain	734:754	a low-c-di-GMP strain	734:754	We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired.					
35916402	4	73	contain	had	756:758	arg2	defect					776:781	no colonization defect	760:781	no colonization defect	760:781	We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired.					
35916402	3	74	theme	c-di-GMP	546:553	arg1	regulation					555:564	c-di-GMP regulation	546:564	c-di-GMP regulation	546:564	V. fischeri encodes 50 proteins predicted to synthesize and/or degrade c-di-GMP, but a role for c-di-GMP regulation during host colonization has not been investigated.					
35916402	1	75	theme	lifestyle	190:198	arg1	transition					200:209	a lifestyle transition	188:209	a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus	188:292	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	2	76	theme	Cyclic	295:300	arg1	important					369:377	important	369:377	important	369:377	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	2	76	theme	Cyclic	295:300	arg1	molecule					352:359	a cytoplasmic signaling molecule	328:359	a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species	328:447	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	2	76	theme	Cyclic	295:300	arg1	c-di-GMP					315:322	c-di-GMP	315:322	c-di-GMP	315:322	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	2	76	theme	Cyclic	295:300	arg1	diguanylate					302:312	Cyclic diguanylate	295:312	Cyclic diguanylate (c-di-GMP)	295:323	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	8	77	theme	substantial	1520:1530	arg1	interest					1532:1539	substantial interest	1520:1539	substantial interest in studying cyclic diguanylate (c-di-GMP) in pathogenic and environmental bacteria, which has led to an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility	1520:1737	IMPORTANCE There is substantial interest in studying cyclic diguanylate (c-di-GMP) in pathogenic and environmental bacteria, which has led to an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility.					
35916402	7	78	theme	systems	1461:1467	arg1	interplay					1421:1429	an interplay	1418:1429	an interplay of multiple exopolysaccharide systems in host-associated aggregates	1418:1497	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	10	79	theme	track	2046:2050	arg1	phenotypes					2061:2070	track c-di-GMP phenotypes	2046:2070	track c-di-GMP phenotypes	2046:2070	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	10	80	theme	study	1913:1917	arg1	system					1919:1924	the Vibrio fischeri-Hawaiian bobtail squid study system	1870:1924	the Vibrio fischeri-Hawaiian bobtail squid study system	1870:1924	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	7	81	theme	multiple	1434:1441	arg1	systems					1461:1467	multiple exopolysaccharide systems	1434:1467	multiple exopolysaccharide systems in host-associated aggregates	1434:1497	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	1	82	from	state	236:240	arg1	transition					200:209	a lifestyle transition	188:209	a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus	188:292	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	1	82	from	state	236:240	arg1	environment					249:259	the environment	245:259	the environment	245:259	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	10	83	theme	bobtail	1899:1905	arg1	system					1919:1924	the Vibrio fischeri-Hawaiian bobtail squid study system	1870:1924	the Vibrio fischeri-Hawaiian bobtail squid study system	1870:1924	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	1	84	theme	bobtail	120:126	arg1	squid					128:132	the Hawaiian bobtail squid	107:132	the Hawaiian bobtail squid (Euprymna scolopes)	107:152	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	1	84	theme	bobtail	120:126	arg1	scolopes					144:151	Euprymna scolopes	135:151	Euprymna scolopes	135:151	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	8	85	theme	cyclic	1553:1558	arg1	c-di-GMP					1573:1580	c-di-GMP	1573:1580	c-di-GMP	1573:1580	IMPORTANCE There is substantial interest in studying cyclic diguanylate (c-di-GMP) in pathogenic and environmental bacteria, which has led to an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility.					
35916402	8	85	theme	cyclic	1553:1558	arg1	diguanylate					1560:1570	cyclic diguanylate	1553:1570	cyclic diguanylate (c-di-GMP)	1553:1581	IMPORTANCE There is substantial interest in studying cyclic diguanylate (c-di-GMP) in pathogenic and environmental bacteria, which has led to an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility.					
35916402	7	86	from	systems	1461:1467	arg1	aggregates					1488:1497	host-associated aggregates	1472:1497	host-associated aggregates	1472:1497	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	11	87	theme	biofilm	2198:2204	arg1	cellulose					2231:2239	cellulose	2231:2239	cellulose	2231:2239	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	11	87	theme	biofilm	2198:2204	arg1	Syp					2223:2225	Syp	2223:2225	Syp	2223:2225	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	11	87	theme	biofilm	2198:2204	arg1	polysaccharides					2206:2220	two distinct biofilm polysaccharides	2185:2220	two distinct biofilm polysaccharides	2185:2220	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	6	88	theme	polysaccharide	1220:1233	arg1	presence					1198:1205	the presence	1194:1205	the presence of the other polysaccharide	1194:1233	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	4	89	theme	c-di-GMP	678:685	arg1	levels					668:673	either low or high levels	649:673	either low or high levels of c-di-GMP	649:685	We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired.					
35916402	6	90	theme	V.	1023:1024	arg1	fischeri					1026:1033	colonizing V. fischeri	1012:1033	colonizing V. fischeri	1012:1033	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	8	91	theme	pathogenic	1586:1595	arg1	bacteria					1615:1622	pathogenic and environmental bacteria	1586:1622	pathogenic and environmental bacteria	1586:1622	IMPORTANCE There is substantial interest in studying cyclic diguanylate (c-di-GMP) in pathogenic and environmental bacteria, which has led to an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility.					
35916402	4	92	theme	squid	694:698	arg1	colonization					700:711	squid colonization	694:711	squid colonization	694:711	We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired.					
35916402	12	93	theme	important	2457:2465	arg1	parallels					2467:2475	important parallels	2457:2475	important parallels between pathogenic and beneficial colonization	2457:2522	This study reveals important parallels between pathogenic and beneficial colonization and additionally identifies c-di-GMP-dependent regulation that occurs specifically in the squid host.					
35916402	11	94	theme	biofilm	2360:2366	arg1	abundance					2335:2343	abundance	2335:2343	abundance	2335:2343	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	11	94	theme	biofilm	2360:2366	arg1	composition					2319:2329	composition	2319:2329	composition	2319:2329	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	8	95	theme	environmental	1601:1613	arg1	bacteria					1615:1622	pathogenic and environmental bacteria	1586:1622	pathogenic and environmental bacteria	1586:1622	IMPORTANCE There is substantial interest in studying cyclic diguanylate (c-di-GMP) in pathogenic and environmental bacteria, which has led to an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility.					
35916402	11	96	theme	in	2352:2353	arg1	biofilm					2360:2366	the in vivo biofilm	2348:2366	the in vivo biofilm	2348:2366	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	11	97	from	evident	2255:2261	arg1	studies					2280:2286	culture-based studies	2266:2286	culture-based studies	2266:2286	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	5	98	theme	high	949:952	arg1	levels					963:968	high c-di-GMP levels	949:968	high c-di-GMP levels	949:968	Expression of a heterologous c-di-GMP phosphodiesterase restored colonization, demonstrating that the effect is due to high c-di-GMP levels.					
35916402	0	99	theme	Cyclic	15:20	arg1	Diguanylate					22:32	Cyclic Diguanylate	15:32	Cyclic Diguanylate	15:32	High Levels of Cyclic Diguanylate Interfere with Beneficial Bacterial Colonization.					
35916402	7	100	from	interplay	1421:1429	arg1	aggregates					1488:1497	host-associated aggregates	1472:1497	host-associated aggregates	1472:1497	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	6	101	theme	aggregate	1079:1087	arg1	morphology					1089:1098	altered biofilm aggregate morphology	1063:1098	altered biofilm aggregate morphology	1063:1098	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	2	102	theme	many	426:429	arg1	species					441:447	many bacterial species	426:447	many bacterial species	426:447	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	10	103	theme	genetic	1955:1961	arg1	advances					1963:1970	recent genetic advances	1948:1970	recent genetic advances in the bacterium	1948:1987	Using the Vibrio fischeri-Hawaiian bobtail squid study system, we took advantage of recent genetic advances in the bacterium to modulate c-di-GMP levels and measure colonization and track c-di-GMP phenotypes in a symbiotic interaction.					
35916402	1	104	theme	host	283:286	arg1	mucus					288:292	host mucus	283:292	host mucus	283:292	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	6	105	theme	altered	1063:1069	arg1	morphology					1089:1098	altered biofilm aggregate morphology	1063:1098	altered biofilm aggregate morphology	1063:1098	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	8	106	theme	accepted	1645:1652	arg1	paradigm					1654:1661	an accepted paradigm	1642:1661	an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility	1642:1737	IMPORTANCE There is substantial interest in studying cyclic diguanylate (c-di-GMP) in pathogenic and environmental bacteria, which has led to an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility.					
35916402	0	107	theme	Beneficial	49:58	arg1	Colonization					70:81	Beneficial Bacterial Colonization	49:81	Beneficial Bacterial Colonization	49:81	High Levels of Cyclic Diguanylate Interfere with Beneficial Bacterial Colonization.					
35916402	6	108	theme	locus	1172:1176	arg1	transcription					1136:1148	transcription	1136:1148	transcription of one polysaccharide locus	1136:1176	In the constitutive high-c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a regulatory interaction where transcription of one polysaccharide locus is inhibited by the presence of the other polysaccharide.					
35916402	11	109	dep	in	2352:2353	arg1	vivo					2355:2358	vivo	2355:2358	vivo	2355:2358	Studies in the animal host revealed a c-di-GMP-dependent genetic interaction between two distinct biofilm polysaccharides, Syp and cellulose, that was not evident in culture-based studies: elevated c-di-GMP altered the composition and abundance of the in vivo biofilm by decreasing syp transcription due to increased cellulose synthesis.					
35916402	7	110	theme	beneficial	1310:1319	arg1	colonization					1328:1339	beneficial animal colonization	1310:1339	beneficial animal colonization	1310:1339	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	8	111	theme	high	1672:1675	arg1	levels					1686:1691	high c-di-GMP levels	1672:1691	high c-di-GMP levels	1672:1691	IMPORTANCE There is substantial interest in studying cyclic diguanylate (c-di-GMP) in pathogenic and environmental bacteria, which has led to an accepted paradigm in which high c-di-GMP levels promote biofilm formation and reduce motility.					
35916402	1	112	theme	motile	229:234	arg1	state					236:240	a planktonic motile state	216:240	a planktonic motile state in the environment	216:259	During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus.					
35916402	7	113	from	c-di-GMP	1384:1391	arg1	host					1400:1403	the host	1396:1403	the host	1396:1403	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	7	114	theme	regulation	1292:1301	arg1	importance					1262:1271	the importance	1258:1271	the importance of proper c-di-GMP regulation during beneficial animal colonization	1258:1339	Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.					
35916402	12	115	theme	c-di-GMP-dependent	2552:2569	arg1	regulation					2571:2580	c-di-GMP-dependent regulation	2552:2580	c-di-GMP-dependent regulation that occurs specifically in the squid host	2552:2623	This study reveals important parallels between pathogenic and beneficial colonization and additionally identifies c-di-GMP-dependent regulation that occurs specifically in the squid host.					
35916402	4	116	theme	c-di-GMP	791:798	arg1	strain					800:805	a high c-di-GMP strain	784:805	a high c-di-GMP strain	784:805	We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired.					
35916402	4	116	theme	c-di-GMP	791:798	arg1	impaired					820:827	impaired	820:827	impaired	820:827	We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low-c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired.					
35916402	2	117	theme	signaling	342:350	arg1	important					369:377	important	369:377	important	369:377	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	2	117	theme	signaling	342:350	arg1	diguanylate					302:312	Cyclic diguanylate	295:312	Cyclic diguanylate (c-di-GMP)	295:323	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
35916402	2	117	theme	signaling	342:350	arg1	molecule					352:359	a cytoplasmic signaling molecule	328:359	a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species	328:447	Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species.					
37097067	4	0	theme	thermal	816:822	arg1	difference					849:858	∼5°C temperature difference	832:858	∼5°C temperature difference	832:858	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	4	0	theme	thermal	816:822	arg1	damage					824:829	thermal damage	816:829	thermal damage (∼5°C temperature difference)	816:859	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	2	1	theme	shape	349:353	arg1	recovery					355:362	shape recovery	349:362	shape recovery	349:362	Herein, AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery are prepared to minimize blood loss, kill bacteria, and promote wound healing.					
37097067	6	2	with	AgZ-CB	1134:1139	arg1	biocompatibility					1156:1171	excellent biocompatibility	1146:1171	excellent biocompatibility	1146:1171	The in vivo wound healing and biodegradation experiment indicates that AgZ-CB with excellent biocompatibility can close wounds efficiently.					
37097067	5	3	dep	less	896:899	arg1	loss					907:910	blood loss	901:910	blood loss (26 mg)	901:918	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	5	3	dep	less	896:899	arg1	s					957:957	42 s	954:957	42 s	954:957	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	5	3	dep	less	896:899	arg1	time					934:937	a shorter time	924:937	a shorter time to hemostasis (42 s)	924:958	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	5	3	dep	less	896:899	arg1	26 mg					913:917	26 mg	913:917	26 mg	913:917	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	5	4	theme	polyvinyl	980:988	arg1	s					1018:1018	84 mg and 76 s	1005:1018	84 mg and 76 s	1005:1018	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	5	4	theme	polyvinyl	980:988	arg1	sponge					997:1002	the commercial polyvinyl formal sponge	965:1002	the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model	965:1060	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	5	5	theme	shorter	926:932	arg1	s					957:957	42 s	954:957	42 s	954:957	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	5	5	theme	shorter	926:932	arg1	time					934:937	a shorter time	924:937	a shorter time to hemostasis (42 s)	924:958	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	4	6	dep	exhibits	619:626	arg1	rate					676:679	∼100% bactericidal rate	657:679	∼100% bactericidal rate	657:679	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	5	7	theme	blood	901:905	arg1	loss					907:910	blood loss	901:910	blood loss (26 mg)	901:918	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	5	7	theme	blood	901:905	arg1	26 mg					913:917	26 mg	913:917	26 mg	913:917	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	0	8	theme	Heat	111:114	arg1	Injury					116:121	Heat Injury	111:121	Heat Injury	111:121	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.					
37097067	6	9	theme	healing	1081:1087	arg1	experiment					1108:1117	The in vivo wound healing and biodegradation experiment	1063:1117	The in vivo wound healing and biodegradation experiment	1063:1117	The in vivo wound healing and biodegradation experiment indicates that AgZ-CB with excellent biocompatibility can close wounds efficiently.					
37097067	2	10	theme	AgZ-CB	318:323	arg1	sponges					336:342	AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges	290:342	AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery	290:362	Herein, AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery are prepared to minimize blood loss, kill bacteria, and promote wound healing.					
37097067	6	11	dep	in	1067:1068	arg1	vivo					1070:1073	vivo	1070:1073	vivo	1070:1073	The in vivo wound healing and biodegradation experiment indicates that AgZ-CB with excellent biocompatibility can close wounds efficiently.					
37097067	7	12	theme	AgZ-CB	1216:1221	arg1	sponge					1223:1228	the AgZ-CB sponge	1212:1228	the AgZ-CB sponge	1212:1228	Overall, the AgZ-CB sponge has great potential in combating a series of obstacles in wound healing.					
37097067	2	13	theme	blood	389:393	arg1	loss					395:398	blood loss	389:398	blood loss	389:398	Herein, AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery are prepared to minimize blood loss, kill bacteria, and promote wound healing.					
37097067	6	14	theme	excellent	1146:1154	arg1	biocompatibility					1156:1171	excellent biocompatibility	1146:1171	excellent biocompatibility	1146:1171	The in vivo wound healing and biodegradation experiment indicates that AgZ-CB with excellent biocompatibility can close wounds efficiently.					
37097067	4	15	theme	AgZ-CB	605:610	arg1	sponge					612:617	The fabricated AgZ-CB sponge	590:617	The fabricated AgZ-CB sponge	590:617	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	0	16	theme	Antibacterial	16:28	arg1	Sponge					78:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge	0:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.	0:122	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.					
37097067	3	17	theme	first	486:490	arg1	time					492:495	the first time	482:495	the first time	482:495	Notably, the bamboo powder is used for the first time to remarkably enhance the softness of the composite sponge (volumetric expansion ratio > 5).					
37097067	5	18	theme	AgZ-CB	876:881	arg1	sponge					883:888	the AgZ-CB sponge	872:888	the AgZ-CB sponge	872:888	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	1	19	theme	many	185:188	arg1	deficiencies					190:201	many deficiencies	185:201	many deficiencies in its ability to effectively stop bleeding, prevent infection and fit wounds	185:279	Chitin is a popular hemostatic material, but there are still many deficiencies in its ability to effectively stop bleeding, prevent infection and fit wounds.					
37097067	0	20	theme	High-Efficiency	0:14	arg1	Sponge					78:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge	0:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.	0:122	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.					
37097067	6	21	theme	wound	1075:1079	arg1	healing					1081:1087	in vivo wound healing	1067:1087	in vivo wound healing	1067:1087	The in vivo wound healing and biodegradation experiment indicates that AgZ-CB with excellent biocompatibility can close wounds efficiently.					
37097067	4	22	theme	bactericidal	663:674	arg1	rate					676:679	∼100% bactericidal rate	657:679	∼100% bactericidal rate	657:679	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	2	23	theme	zeolite/chitin/bamboo	295:315	arg1	sponges					336:342	AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges	290:342	AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery	290:362	Herein, AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery are prepared to minimize blood loss, kill bacteria, and promote wound healing.					
37097067	7	24	from	series	1265:1270	arg1	healing					1294:1300	wound healing	1288:1300	wound healing	1288:1300	Overall, the AgZ-CB sponge has great potential in combating a series of obstacles in wound healing.					
37097067	0	25	theme	AgNP	41:44	arg1	Sponge					78:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge	0:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.	0:122	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.					
37097067	4	26	theme	internal	732:739	arg1	pathways					766:773	internal and external coagulation pathways	732:773	internal and external coagulation pathways	732:773	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	4	27	theme	coagulation	754:764	arg1	pathways					766:773	internal and external coagulation pathways	732:773	internal and external coagulation pathways	732:773	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	2	28	theme	@	294:294	arg1	sponges					336:342	AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges	290:342	AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery	290:362	Herein, AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery are prepared to minimize blood loss, kill bacteria, and promote wound healing.					
37097067	7	29	theme	obstacles	1275:1283	arg1	series					1265:1270	a series	1263:1270	a series of obstacles in wound healing	1263:1300	Overall, the AgZ-CB sponge has great potential in combating a series of obstacles in wound healing.					
37097067	0	30	theme	Hemostatic	30:39	arg1	Sponge					78:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge	0:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.	0:122	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.					
37097067	6	31	theme	in	1067:1068	arg1	healing					1081:1087	in vivo wound healing	1067:1087	in vivo wound healing	1067:1087	The in vivo wound healing and biodegradation experiment indicates that AgZ-CB with excellent biocompatibility can close wounds efficiently.					
37097067	1	32	dep	bleeding	238:245	arg1	to					218:219	to	218:219	to	218:219	Chitin is a popular hemostatic material, but there are still many deficiencies in its ability to effectively stop bleeding, prevent infection and fit wounds.					
37097067	2	33	theme	AgNP	290:293	arg1	sponges					336:342	AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges	290:342	AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery	290:362	Herein, AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery are prepared to minimize blood loss, kill bacteria, and promote wound healing.					
37097067	7	34	from	obstacles	1275:1283	arg1	healing					1294:1300	wound healing	1288:1300	wound healing	1288:1300	Overall, the AgZ-CB sponge has great potential in combating a series of obstacles in wound healing.					
37097067	0	35	theme	Zeolite/Chitin/Bamboo	46:66	arg1	Sponge					78:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge	0:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.	0:122	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.					
37097067	4	36	theme	∼100	657:660	arg1	%					661:661	%	661:661	%	661:661	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	5	37	theme	commercial	969:978	arg1	s					1018:1018	84 mg and 76 s	1005:1018	84 mg and 76 s	1005:1018	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	5	37	theme	commercial	969:978	arg1	sponge					997:1002	the commercial polyvinyl formal sponge	965:1002	the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model	965:1060	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	4	38	theme	external	745:752	arg1	pathways					766:773	internal and external coagulation pathways	732:773	internal and external coagulation pathways	732:773	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	4	39	theme	fabricated	594:603	arg1	sponge					612:617	The fabricated AgZ-CB sponge	590:617	The fabricated AgZ-CB sponge	590:617	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	0	40	theme	@	45:45	arg1	Sponge					78:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge	0:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.	0:122	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.					
37097067	7	41	from	healing	1294:1300	arg1	series					1265:1270	a series	1263:1270	a series of obstacles in wound healing	1263:1300	Overall, the AgZ-CB sponge has great potential in combating a series of obstacles in wound healing.					
37097067	2	42	with	sponges	336:342	arg1	recovery					355:362	shape recovery	349:362	shape recovery	349:362	Herein, AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery are prepared to minimize blood loss, kill bacteria, and promote wound healing.					
37097067	3	43	theme	volumetric	557:566	arg1	>					584:584	volumetric expansion ratio > 5	557:586	volumetric expansion ratio > 5	557:586	Notably, the bamboo powder is used for the first time to remarkably enhance the softness of the composite sponge (volumetric expansion ratio > 5).					
37097067	3	43	theme	volumetric	557:566	arg1	sponge					549:554	the composite sponge	535:554	the composite sponge (volumetric expansion ratio > 5)	535:587	Notably, the bamboo powder is used for the first time to remarkably enhance the softness of the composite sponge (volumetric expansion ratio > 5).					
37097067	5	44	theme	full-thickness	1028:1041	arg1	injury					1049:1054	the full-thickness liver injury	1024:1054	the full-thickness liver injury model	1024:1060	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	4	45	theme	temperature	837:847	arg1	difference					849:858	∼5°C temperature difference	832:858	∼5°C temperature difference	832:858	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	4	45	theme	temperature	837:847	arg1	damage					824:829	thermal damage	816:829	thermal damage (∼5°C temperature difference)	816:859	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	5	46	theme	formal	990:995	arg1	s					1018:1018	84 mg and 76 s	1005:1018	84 mg and 76 s	1005:1018	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	5	46	theme	formal	990:995	arg1	sponge					997:1002	the commercial polyvinyl formal sponge	965:1002	the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model	965:1060	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	3	47	theme	composite	539:547	arg1	>					584:584	volumetric expansion ratio > 5	557:586	volumetric expansion ratio > 5	557:586	Notably, the bamboo powder is used for the first time to remarkably enhance the softness of the composite sponge (volumetric expansion ratio > 5).					
37097067	3	47	theme	composite	539:547	arg1	sponge					549:554	the composite sponge	535:554	the composite sponge (volumetric expansion ratio > 5)	535:587	Notably, the bamboo powder is used for the first time to remarkably enhance the softness of the composite sponge (volumetric expansion ratio > 5).					
37097067	4	48	theme	%	661:661	arg1	rate					676:679	∼100% bactericidal rate	657:679	∼100% bactericidal rate	657:679	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	0	49	theme	Composite	68:76	arg1	Sponge					78:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge	0:83	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.	0:122	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.					
37097067	5	50	theme	liver	1043:1047	arg1	injury					1049:1054	the full-thickness liver injury	1024:1054	the full-thickness liver injury model	1024:1060	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	4	51	theme	∼5°C	832:835	arg1	difference					849:858	∼5°C temperature difference	832:858	∼5°C temperature difference	832:858	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	4	51	theme	∼5°C	832:835	arg1	damage					824:829	thermal damage	816:829	thermal damage (∼5°C temperature difference)	816:859	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	3	52	theme	sponge	549:554	arg1	softness					523:530	the softness	519:530	the softness of the composite sponge (volumetric expansion ratio > 5)	519:587	Notably, the bamboo powder is used for the first time to remarkably enhance the softness of the composite sponge (volumetric expansion ratio > 5).					
37097067	7	53	theme	great	1234:1238	arg1	potential					1240:1248	great potential	1234:1248	great potential	1234:1248	Overall, the AgZ-CB sponge has great potential in combating a series of obstacles in wound healing.					
37097067	2	54	theme	composite	326:334	arg1	sponges					336:342	AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges	290:342	AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery	290:362	Herein, AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery are prepared to minimize blood loss, kill bacteria, and promote wound healing.					
37097067	6	55	theme	biodegradation	1093:1106	arg1	experiment					1108:1117	The in vivo wound healing and biodegradation experiment	1063:1117	The in vivo wound healing and biodegradation experiment	1063:1117	The in vivo wound healing and biodegradation experiment indicates that AgZ-CB with excellent biocompatibility can close wounds efficiently.					
37097067	5	56	theme	injury	1049:1054	arg1	model					1056:1060	the full-thickness liver injury model	1024:1060	the full-thickness liver injury model	1024:1060	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	4	57	theme	excellent	631:639	arg1	effect					649:654	an excellent killing effect	628:654	an excellent killing effect	628:654	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	1	58	theme	popular	136:142	arg1	material					155:162	a popular hemostatic material	134:162	a popular hemostatic material	134:162	Chitin is a popular hemostatic material, but there are still many deficiencies in its ability to effectively stop bleeding, prevent infection and fit wounds.					
37097067	1	58	theme	popular	136:142	arg1	Chitin					124:129	Chitin	124:129	Chitin	124:129	Chitin is a popular hemostatic material, but there are still many deficiencies in its ability to effectively stop bleeding, prevent infection and fit wounds.					
37097067	7	59	theme	wound	1288:1292	arg1	healing					1294:1300	wound healing	1288:1300	wound healing	1288:1300	Overall, the AgZ-CB sponge has great potential in combating a series of obstacles in wound healing.					
37097067	3	60	used	used	473:476	arg2	powder					463:468	the bamboo powder	452:468	the bamboo powder	452:468	Notably, the bamboo powder is used for the first time to remarkably enhance the softness of the composite sponge (volumetric expansion ratio > 5).					
37097067	1	61	theme	hemostatic	144:153	arg1	material					155:162	a popular hemostatic material	134:162	a popular hemostatic material	134:162	Chitin is a popular hemostatic material, but there are still many deficiencies in its ability to effectively stop bleeding, prevent infection and fit wounds.					
37097067	1	61	theme	hemostatic	144:153	arg1	Chitin					124:129	Chitin	124:129	Chitin	124:129	Chitin is a popular hemostatic material, but there are still many deficiencies in its ability to effectively stop bleeding, prevent infection and fit wounds.					
37097067	0	62	theme	Wound	89:93	arg1	Healing					95:101	Wound Healing	89:101	Wound Healing	89:101	High-Efficiency Antibacterial Hemostatic AgNP@Zeolite/Chitin/Bamboo Composite Sponge for Wound Healing without Heat Injury.					
37097067	5	63	from	sponge	997:1002	arg1	model					1056:1060	the full-thickness liver injury model	1024:1060	the full-thickness liver injury model	1024:1060	Moreover, the AgZ-CB sponge shows less blood loss (26 mg) and a shorter time to hemostasis (42 s) than the commercial polyvinyl formal sponge (84 mg and 76 s) in the full-thickness liver injury model.					
37097067	3	64	theme	expansion	568:576	arg1	>					584:584	volumetric expansion ratio > 5	557:586	volumetric expansion ratio > 5	557:586	Notably, the bamboo powder is used for the first time to remarkably enhance the softness of the composite sponge (volumetric expansion ratio > 5).					
37097067	3	64	theme	expansion	568:576	arg1	sponge					549:554	the composite sponge	535:554	the composite sponge (volumetric expansion ratio > 5)	535:587	Notably, the bamboo powder is used for the first time to remarkably enhance the softness of the composite sponge (volumetric expansion ratio > 5).					
37097067	1	65	from	deficiencies	190:201	arg1	ability					210:216	its ability to effectively stop bleeding, prevent infection and fit wounds	206:279	its ability to effectively stop bleeding, prevent infection and fit wounds	206:279	Chitin is a popular hemostatic material, but there are still many deficiencies in its ability to effectively stop bleeding, prevent infection and fit wounds.					
37097067	4	66	theme	killing	641:647	arg1	effect					649:654	an excellent killing effect	628:654	an excellent killing effect	628:654	The fabricated AgZ-CB sponge exhibits an excellent killing effect (∼100% bactericidal rate) against both E. coli and S. aureus, and activates internal and external coagulation pathways to accelerate hemostasis without causing thermal damage (∼5°C temperature difference).					
37097067	3	67	theme	bamboo	456:461	arg1	powder					463:468	the bamboo powder	452:468	the bamboo powder	452:468	Notably, the bamboo powder is used for the first time to remarkably enhance the softness of the composite sponge (volumetric expansion ratio > 5).					
37097067	3	68	theme	ratio	578:582	arg1	>					584:584	volumetric expansion ratio > 5	557:586	volumetric expansion ratio > 5	557:586	Notably, the bamboo powder is used for the first time to remarkably enhance the softness of the composite sponge (volumetric expansion ratio > 5).					
37097067	3	68	theme	ratio	578:582	arg1	sponge					549:554	the composite sponge	535:554	the composite sponge (volumetric expansion ratio > 5)	535:587	Notably, the bamboo powder is used for the first time to remarkably enhance the softness of the composite sponge (volumetric expansion ratio > 5).					
37097067	2	69	theme	wound	428:432	arg1	healing					434:440	wound healing	428:440	wound healing	428:440	Herein, AgNP@zeolite/chitin/bamboo (AgZ-CB) composite sponges with shape recovery are prepared to minimize blood loss, kill bacteria, and promote wound healing.					
37097067	7	70	contain	has	1230:1232	arg1	sponge					1223:1228	the AgZ-CB sponge	1212:1228	the AgZ-CB sponge	1212:1228	Overall, the AgZ-CB sponge has great potential in combating a series of obstacles in wound healing.					
37097067	7	70	contain	has	1230:1232	arg2	potential					1240:1248	great potential	1234:1248	great potential	1234:1248	Overall, the AgZ-CB sponge has great potential in combating a series of obstacles in wound healing.					
35413312	0	0	theme	content	89:95	arg1	production					70:79	optimized production	60:79	optimized production of high content	60:95	Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.					
35413312	5	1	used	used	1046:1049	arg2	aspect					934:939	visual aspect	927:939	visual aspect	927:939	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	5	1	used	used	1046:1049	arg2	properties					915:924	The morphological and chemical properties	884:924	The morphological and chemical properties	884:924	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	5	1	used	used	1046:1049	arg2	stability					945:953	stability	945:953	stability	945:953	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	4	2	theme	lignin	860:865	arg1	LCNF					867:870	high content lignin LCNF	847:870	high content lignin LCNF production	847:881	This study aimed to evaluate the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production.					
35413312	3	3	theme	negative	527:534	arg1	aspects					536:542	Some negative aspects	522:542	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils	522:613	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	1	4	theme	nanomaterial	257:268	arg1	composition					270:280	the nanomaterial composition	253:280	the nanomaterial composition	253:280	Lignin-cellulose nanofibrils (LCNF) are of attracting an increasing interest due to the benefits of maintaining the lignin in the nanomaterial composition.					
35413312	2	5	theme	environmentally	496:510	arg1	friendly					512:519	more environmentally friendly	491:519	more environmentally friendly	491:519	The production of LCNF requires considerable energy consumption, which has been suppressed employing pretreatment of biomass, in which it highlights those that employ enzymes that have the advantage of being more environmentally friendly.					
35413312	4	6	theme	content	852:858	arg1	LCNF					867:870	high content lignin LCNF	847:870	high content lignin LCNF production	847:881	This study aimed to evaluate the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production.					
35413312	5	7	theme	morphological	888:900	arg1	stability					945:953	stability	945:953	stability	945:953	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	5	7	theme	morphological	888:900	arg1	properties					915:924	The morphological and chemical properties	884:924	The morphological and chemical properties	884:924	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	5	7	theme	morphological	888:900	arg1	aspect					934:939	visual aspect	927:939	visual aspect	927:939	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	6	8	theme	laccase	1081:1087	arg1	loading					1089:1095	The laccase loading	1077:1095	The laccase loading used	1077:1100	The laccase loading used was efficient in modifying the lignin to facilitate the action of the endoglucanase on cellulose without causing the removal of this macromolecule.					
35413312	6	8	theme	laccase	1081:1087	arg1	efficient					1106:1114	efficient	1106:1114	efficient	1106:1114	The laccase loading used was efficient in modifying the lignin to facilitate the action of the endoglucanase on cellulose without causing the removal of this macromolecule.					
35413312	4	9	theme	laccase	817:823	arg1	pretreatment					801:812	a combined enzymatic pretreatment	780:812	a combined enzymatic pretreatment of laccase and endoglucanase	780:841	This study aimed to evaluate the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production.					
35413312	3	10	theme	physical	684:691	arg1	barrier					693:699	a physical barrier	682:699	a physical barrier to the action of cellulase enzymes	682:734	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	3	10	theme	physical	684:691	arg1	it					623:624	it	623:624	it	623:624	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	3	11	from	aspects	536:542	arg1	fiber					577:581	the fiber to obtain cellulose nanofibrils	573:613	the fiber to obtain cellulose nanofibrils	573:613	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	3	12	theme	lignin	563:568	arg1	presence					551:558	the presence	547:558	the presence of lignin in the fiber to obtain cellulose nanofibrils	547:613	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	2	13	theme	considerable	315:326	arg1	consumption					335:345	considerable energy consumption	315:345	considerable energy consumption	315:345	The production of LCNF requires considerable energy consumption, which has been suppressed employing pretreatment of biomass, in which it highlights those that employ enzymes that have the advantage of being more environmentally friendly.					
35413312	4	14	theme	LCNF	867:870	arg1	production					872:881	high content lignin LCNF production	847:881	high content lignin LCNF production	847:881	This study aimed to evaluate the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production.					
35413312	2	15	contain	have	463:466	arg1	enzymes					450:456	enzymes	450:456	enzymes that have the advantage of being more environmentally friendly	450:519	The production of LCNF requires considerable energy consumption, which has been suppressed employing pretreatment of biomass, in which it highlights those that employ enzymes that have the advantage of being more environmentally friendly.					
35413312	2	15	contain	have	463:466	arg2	advantage					472:480	the advantage	468:480	the advantage of being more environmentally friendly	468:519	The production of LCNF requires considerable energy consumption, which has been suppressed employing pretreatment of biomass, in which it highlights those that employ enzymes that have the advantage of being more environmentally friendly.					
35413312	2	16	theme	LCNF	301:304	arg1	production					287:296	The production	283:296	The production of LCNF	283:304	The production of LCNF requires considerable energy consumption, which has been suppressed employing pretreatment of biomass, in which it highlights those that employ enzymes that have the advantage of being more environmentally friendly.					
35413312	3	17	theme	cellulase	718:726	arg1	enzymes					728:734	cellulase enzymes	718:734	cellulase enzymes	718:734	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	4	18	theme	endoglucanase	829:841	arg1	pretreatment					801:812	a combined enzymatic pretreatment	780:812	a combined enzymatic pretreatment of laccase and endoglucanase	780:841	This study aimed to evaluate the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production.					
35413312	3	19	theme	presence	551:558	arg1	aspects					536:542	Some negative aspects	522:542	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils	522:613	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	6	20	from	action	1158:1163	arg1	cellulose					1189:1197	cellulose	1189:1197	cellulose	1189:1197	The laccase loading used was efficient in modifying the lignin to facilitate the action of the endoglucanase on cellulose without causing the removal of this macromolecule.					
35413312	1	21	theme	increasing	184:193	arg1	interest					195:202	an increasing interest	181:202	an increasing interest due to the benefits of maintaining the lignin in the nanomaterial composition	181:280	Lignin-cellulose nanofibrils (LCNF) are of attracting an increasing interest due to the benefits of maintaining the lignin in the nanomaterial composition.					
35413312	7	22	theme	industrial	1408:1417	arg1	production					1419:1428	industrial production	1408:1428	industrial production	1408:1428	This pretreatment improved the quality of LCNF (61 ± 3 to 71 ± 2 points) with an energy saving of 42% and, therefore, this pretreatment could be suitable for industrial production for a variety of applications.					
35413312	0	23	theme	high	84:87	arg1	content					89:95	high content	84:95	high content	84:95	Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.					
35413312	4	24	theme	high	847:850	arg1	LCNF					867:870	high content lignin LCNF	847:870	high content lignin LCNF production	847:881	This study aimed to evaluate the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production.					
35413312	1	25	theme	due	204:206	arg1	interest					195:202	an increasing interest	181:202	an increasing interest due to the benefits of maintaining the lignin in the nanomaterial composition	181:280	Lignin-cellulose nanofibrils (LCNF) are of attracting an increasing interest due to the benefits of maintaining the lignin in the nanomaterial composition.					
35413312	0	26	theme	Eco-friendly	0:11	arg1	laccase					13:19	Eco-friendly laccase	0:19	Eco-friendly laccase	0:19	Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.					
35413312	6	27	theme	macromolecule	1235:1247	arg1	removal					1219:1225	the removal	1215:1225	the removal of this macromolecule	1215:1247	The laccase loading used was efficient in modifying the lignin to facilitate the action of the endoglucanase on cellulose without causing the removal of this macromolecule.					
35413312	0	28	theme	cellulase	25:33	arg1	enzymes					35:41	cellulase enzymes	25:41	cellulase enzymes	25:41	Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.					
35413312	4	29	theme	combined	782:789	arg1	pretreatment					801:812	a combined enzymatic pretreatment	780:812	a combined enzymatic pretreatment of laccase and endoglucanase	780:841	This study aimed to evaluate the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production.					
35413312	2	30	theme	energy	328:333	arg1	consumption					335:345	considerable energy consumption	315:345	considerable energy consumption	315:345	The production of LCNF requires considerable energy consumption, which has been suppressed employing pretreatment of biomass, in which it highlights those that employ enzymes that have the advantage of being more environmentally friendly.					
35413312	7	31	dep	71 ± 2	1308:1313	arg1	to					1305:1306	to	1305:1306	to	1305:1306	This pretreatment improved the quality of LCNF (61 ± 3 to 71 ± 2 points) with an energy saving of 42% and, therefore, this pretreatment could be suitable for industrial production for a variety of applications.					
35413312	3	32	theme	enzymes	728:734	arg1	action					708:713	the action	704:713	the action of cellulase enzymes	704:734	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	5	33	theme	mechanical	971:980	arg1	properties					982:991	mechanical properties	971:991	mechanical properties	971:991	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	1	34	theme	Lignin-cellulose	127:142	arg1	LCNF					157:160	LCNF	157:160	LCNF	157:160	Lignin-cellulose nanofibrils (LCNF) are of attracting an increasing interest due to the benefits of maintaining the lignin in the nanomaterial composition.					
35413312	1	34	theme	Lignin-cellulose	127:142	arg1	nanofibrils					144:154	Lignin-cellulose nanofibrils	127:154	Lignin-cellulose nanofibrils (LCNF)	127:161	Lignin-cellulose nanofibrils (LCNF) are of attracting an increasing interest due to the benefits of maintaining the lignin in the nanomaterial composition.					
35413312	5	35	theme	quality	1027:1033	arg1	index					1035:1039	quality index	1027:1039	quality index	1027:1039	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	3	36	theme	cell	661:664	arg1	wall					666:669	the cell wall	657:669	the cell wall	657:669	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	4	37	theme	pretreatment	801:812	arg1	impact					770:775	the impact	766:775	the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production	766:881	This study aimed to evaluate the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production.					
35413312	3	38	attach	presence	551:558	arg1	fiber					577:581	the fiber to obtain cellulose nanofibrils	573:613	the fiber to obtain cellulose nanofibrils	573:613	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	3	38	attach	presence	551:558	arg2	lignin					563:568	lignin	563:568	lignin	563:568	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	7	39	theme	LCNF	1292:1295	arg1	quality					1281:1287	the quality	1277:1287	the quality of LCNF (61 ± 3 to 71 ± 2 points)	1277:1321	This pretreatment improved the quality of LCNF (61 ± 3 to 71 ± 2 points) with an energy saving of 42% and, therefore, this pretreatment could be suitable for industrial production for a variety of applications.					
35413312	4	40	theme	enzymatic	791:799	arg1	pretreatment					801:812	a combined enzymatic pretreatment	780:812	a combined enzymatic pretreatment of laccase and endoglucanase	780:841	This study aimed to evaluate the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production.					
35413312	0	41	theme	optimized	60:68	arg1	production					70:79	optimized production	60:79	optimized production of high content	60:95	Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.					
35413312	3	42	theme	cellulose	593:601	arg1	nanofibrils					603:613	cellulose nanofibrils	593:613	cellulose nanofibrils	593:613	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	5	43	theme	chemical	906:913	arg1	stability					945:953	stability	945:953	stability	945:953	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	5	43	theme	chemical	906:913	arg1	properties					915:924	The morphological and chemical properties	884:924	The morphological and chemical properties	884:924	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	5	43	theme	chemical	906:913	arg1	aspect					934:939	visual aspect	927:939	visual aspect	927:939	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	7	44	theme	%	1350:1350	arg1	saving					1338:1343	an energy saving	1328:1343	an energy saving of 42%	1328:1350	This pretreatment improved the quality of LCNF (61 ± 3 to 71 ± 2 points) with an energy saving of 42% and, therefore, this pretreatment could be suitable for industrial production for a variety of applications.					
35413312	7	45	theme	energy	1331:1336	arg1	saving					1338:1343	an energy saving	1328:1343	an energy saving of 42%	1328:1350	This pretreatment improved the quality of LCNF (61 ± 3 to 71 ± 2 points) with an energy saving of 42% and, therefore, this pretreatment could be suitable for industrial production for a variety of applications.					
35413312	3	46	from	fiber	577:581	arg1	aspects					536:542	Some negative aspects	522:542	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils	522:613	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	1	47	from	lignin	243:248	arg1	composition					270:280	the nanomaterial composition	253:280	the nanomaterial composition	253:280	Lignin-cellulose nanofibrils (LCNF) are of attracting an increasing interest due to the benefits of maintaining the lignin in the nanomaterial composition.					
35413312	3	48	theme	wall	666:669	arg1	delamination					641:652	the delamination	637:652	the delamination of the cell wall	637:669	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
35413312	6	49	theme	endoglucanase	1172:1184	arg1	action					1158:1163	the action	1154:1163	the action of the endoglucanase on cellulose	1154:1197	The laccase loading used was efficient in modifying the lignin to facilitate the action of the endoglucanase on cellulose without causing the removal of this macromolecule.					
35413312	5	50	theme	visual	927:932	arg1	properties					915:924	The morphological and chemical properties	884:924	The morphological and chemical properties	884:924	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	5	50	theme	visual	927:932	arg1	aspect					934:939	visual aspect	927:939	visual aspect	927:939	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	0	51	dep	laccase	13:19	arg1	pretreatment					43:54	pretreatment	43:54	pretreatment for optimized production of high content	43:95	Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.					
35413312	2	52	theme	biomass	400:406	arg1	pretreatment					384:395	pretreatment	384:395	pretreatment	384:395	The production of LCNF requires considerable energy consumption, which has been suppressed employing pretreatment of biomass, in which it highlights those that employ enzymes that have the advantage of being more environmentally friendly.					
35413312	7	53	theme	applications	1447:1458	arg1	applications					1447:1458	applications	1447:1458	applications	1447:1458	This pretreatment improved the quality of LCNF (61 ± 3 to 71 ± 2 points) with an energy saving of 42% and, therefore, this pretreatment could be suitable for industrial production for a variety of applications.					
35413312	7	53	theme	applications	1447:1458	arg1	variety					1436:1442	a variety	1434:1442	a variety of applications	1434:1458	This pretreatment improved the quality of LCNF (61 ± 3 to 71 ± 2 points) with an energy saving of 42% and, therefore, this pretreatment could be suitable for industrial production for a variety of applications.					
35413312	5	54	theme	barrier	1004:1010	arg1	properties					1012:1021	barrier properties	1004:1021	barrier properties	1004:1021	The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF.					
35413312	3	55	from	presence	551:558	arg1	fiber					577:581	the fiber to obtain cellulose nanofibrils	573:613	the fiber to obtain cellulose nanofibrils	573:613	Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes.					
37349573	4	0	from	cellulose	607:615	arg1	rich					599:602	rich	599:602	rich	599:602	In our study, we extracted parent cellulose from Ulva lactuca where consecutive treatments extracted cellulose to obtain an insoluble fraction rich in cellulose.					
37349573	3	1	from	environment	406:416	arg1	abundant					390:397	abundant	390:397	abundant	390:397	Algae are abundant in the environment and have a high content of cellulose.					
37349573	5	2	theme	same	650:653	arg1	results					655:661	the same results	646:661	the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks	646:808	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	5	3	dep	Fourier	733:739	arg1	transform					741:749	transform	741:749	transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks	741:808	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	2	4	theme	nanocellulose	352:364	arg1	biosynthesis					366:377	nanocellulose biosynthesis	352:377	nanocellulose biosynthesis	352:377	The macroalgal biomass was used for nanocellulose biosynthesis.					
37349573	5	5	theme	infrared	751:758	arg1	peaks					804:808	infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks	751:808	infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks	751:808	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	1	6	theme	natural	241:247	arg1	reductions					249:258	its natural reductions	237:258	its natural reductions with low toxicity and avoidance of injurious chemicals	237:313	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	4	7	theme	parent	483:488	arg1	cellulose					490:498	parent cellulose	483:498	parent cellulose	483:498	In our study, we extracted parent cellulose from Ulva lactuca where consecutive treatments extracted cellulose to obtain an insoluble fraction rich in cellulose.					
37349573	12	8	theme	important	1915:1923	arg1	material					1933:1940	a very important medical material	1908:1940	a very important medical material that is compatible with sustainable development	1908:1988	These results demonstrate that nanocellulose could be developed as an excellent solution to these challenges, making nanocellulose extracted from natural algae a very important medical material that is compatible with sustainable development.					
37349573	12	8	theme	important	1915:1923	arg1	compatible					1950:1959	compatible	1950:1959	compatible	1950:1959	These results demonstrate that nanocellulose could be developed as an excellent solution to these challenges, making nanocellulose extracted from natural algae a very important medical material that is compatible with sustainable development.					
37349573	5	9	contain	has	642:644	arg2	results					655:661	the same results	646:661	the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks	646:808	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	5	9	contain	has	642:644	arg1	cellulose					632:640	The extracted cellulose	618:640	The extracted cellulose	618:640	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	4	10	from	rich	599:602	arg1	cellulose					607:615	cellulose	607:615	cellulose	607:615	In our study, we extracted parent cellulose from Ulva lactuca where consecutive treatments extracted cellulose to obtain an insoluble fraction rich in cellulose.					
37349573	12	11	theme	medical	1925:1931	arg1	material					1933:1940	a very important medical material	1908:1940	a very important medical material that is compatible with sustainable development	1908:1988	These results demonstrate that nanocellulose could be developed as an excellent solution to these challenges, making nanocellulose extracted from natural algae a very important medical material that is compatible with sustainable development.					
37349573	12	11	theme	medical	1925:1931	arg1	compatible					1950:1959	compatible	1950:1959	compatible	1950:1959	These results demonstrate that nanocellulose could be developed as an excellent solution to these challenges, making nanocellulose extracted from natural algae a very important medical material that is compatible with sustainable development.					
37349573	9	12	theme	Antibacterial	1154:1166	arg1	examination					1168:1178	Antibacterial examination	1154:1178	Antibacterial examination of nanocellulose	1154:1195	Antibacterial examination of nanocellulose was tested against Gram+ bacteria like Staphylococcus aureus (ATCC6538), Klebsiella pneumonia (ST627), and Gram-negative bacteria such as Escherichia coli (ATCC25922), and coagulase-negative Staphylococci (CoNS) to give 4.06, 4.66, 4.93 and 4.43 cm as respectively.					
37349573	10	13	theme	Inhibitory	1560:1569	arg1	MIC					1586:1588	MIC	1586:1588	MIC	1586:1588	Comparing the antibacterial effect of nanocellulose with some antibiotics and estimating minimal Inhibitory Concentration (MIC) of nanocellulose.					
37349573	10	13	theme	Inhibitory	1560:1569	arg1	Concentration					1571:1583	minimal Inhibitory Concentration	1552:1583	minimal Inhibitory Concentration (MIC) of nanocellulose	1552:1606	Comparing the antibacterial effect of nanocellulose with some antibiotics and estimating minimal Inhibitory Concentration (MIC) of nanocellulose.					
37349573	7	14	theme	slab-like	976:984	arg1	region					986:991	a slab-like region	974:991	a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX)	974:1036	Nanocellulose was examined by Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition.					
37349573	4	15	theme	rich	599:602	arg1	fraction					590:597	an insoluble fraction	577:597	an insoluble fraction rich in cellulose	577:615	In our study, we extracted parent cellulose from Ulva lactuca where consecutive treatments extracted cellulose to obtain an insoluble fraction rich in cellulose.					
37349573	10	16	theme	minimal	1552:1558	arg1	MIC					1586:1588	MIC	1586:1588	MIC	1586:1588	Comparing the antibacterial effect of nanocellulose with some antibiotics and estimating minimal Inhibitory Concentration (MIC) of nanocellulose.					
37349573	10	16	theme	minimal	1552:1558	arg1	Concentration					1571:1583	minimal Inhibitory Concentration	1552:1583	minimal Inhibitory Concentration (MIC) of nanocellulose	1552:1606	Comparing the antibacterial effect of nanocellulose with some antibiotics and estimating minimal Inhibitory Concentration (MIC) of nanocellulose.					
37349573	11	17	theme	nanocellulose	1650:1662	arg1	influence					1623:1631	the influence	1619:1631	the influence of cellulose and nanocellulose on some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis	1619:1745	We tested the influence of cellulose and nanocellulose on some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis.					
37349573	7	18	theme	dispersive	1015:1024	arg1	EDX					1033:1035	EDX	1033:1035	EDX	1033:1035	Nanocellulose was examined by Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition.					
37349573	7	18	theme	dispersive	1015:1024	arg1	X-ray					1026:1030	Energy dispersive X-ray	1008:1030	Energy dispersive X-ray (EDX)	1008:1036	Nanocellulose was examined by Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition.					
37349573	3	19	contain	have	422:425	arg1	Algae					380:384	Algae	380:384	Algae	380:384	Algae are abundant in the environment and have a high content of cellulose.					
37349573	3	19	contain	have	422:425	arg2	content					434:440	a high content	427:440	a high content of cellulose	427:453	Algae are abundant in the environment and have a high content of cellulose.					
37349573	1	20	theme	low	265:267	arg1	toxicity					269:276	low toxicity	265:276	low toxicity	265:276	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	5	21	theme	extracted	622:630	arg1	cellulose					632:640	The extracted cellulose	618:640	The extracted cellulose	618:640	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	11	22	theme	cellulose	1636:1644	arg1	influence					1623:1631	the influence	1619:1631	the influence of cellulose and nanocellulose on some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis	1619:1745	We tested the influence of cellulose and nanocellulose on some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis.					
37349573	0	23	theme	antimicrobial	97:109	arg1	polymer					111:117	antimicrobial polymer	97:117	antimicrobial polymer	97:117	Biosynthesis of cellulose from Ulva lactuca, manufacture of nanocellulose and its application as antimicrobial polymer.					
37349573	0	24	from	lactuca	36:42	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis of cellulose from Ulva lactuca	0:42	Biosynthesis of cellulose from Ulva lactuca, manufacture of nanocellulose and its application as antimicrobial polymer.					
37349573	0	24	from	lactuca	36:42	arg1	application					82:92	its application	78:92	its application as antimicrobial polymer	78:117	Biosynthesis of cellulose from Ulva lactuca, manufacture of nanocellulose and its application as antimicrobial polymer.					
37349573	0	24	from	lactuca	36:42	arg1	manufacture					45:55	manufacture	45:55	manufacture of nanocellulose	45:72	Biosynthesis of cellulose from Ulva lactuca, manufacture of nanocellulose and its application as antimicrobial polymer.					
37349573	4	25	theme	insoluble	580:588	arg1	fraction					590:597	an insoluble fraction	577:597	an insoluble fraction rich in cellulose	577:615	In our study, we extracted parent cellulose from Ulva lactuca where consecutive treatments extracted cellulose to obtain an insoluble fraction rich in cellulose.					
37349573	9	26	theme	nanocellulose	1183:1195	arg1	examination					1168:1178	Antibacterial examination	1154:1178	Antibacterial examination of nanocellulose	1154:1195	Antibacterial examination of nanocellulose was tested against Gram+ bacteria like Staphylococcus aureus (ATCC6538), Klebsiella pneumonia (ST627), and Gram-negative bacteria such as Escherichia coli (ATCC25922), and coagulase-negative Staphylococci (CoNS) to give 4.06, 4.66, 4.93 and 4.43 cm as respectively.					
37349573	11	27	theme	Candida	1728:1734	arg1	tropicalis					1736:1745	Candida tropicalis	1728:1745	Candida tropicalis	1728:1745	We tested the influence of cellulose and nanocellulose on some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis.					
37349573	12	28	theme	sustainable	1966:1976	arg1	development					1978:1988	sustainable development	1966:1988	sustainable development	1966:1988	These results demonstrate that nanocellulose could be developed as an excellent solution to these challenges, making nanocellulose extracted from natural algae a very important medical material that is compatible with sustainable development.					
37349573	8	29	theme	50 nm	1117:1121	arg1	range					1108:1112	the range	1104:1112	the range of 50 nm	1104:1121	The size of nanocellulose in the range of 50 nm is calculated by XRD analysis.					
37349573	7	30	theme	electron	939:946	arg1	microscope					948:957	Scanning electron microscope	930:957	Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition	930:1072	Nanocellulose was examined by Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition.					
37349573	7	30	theme	electron	939:946	arg1	SEM					960:962	SEM	960:962	SEM	960:962	Nanocellulose was examined by Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition.					
37349573	12	31	theme	excellent	1818:1826	arg1	nanocellulose					1779:1791	nanocellulose	1779:1791	nanocellulose	1779:1791	These results demonstrate that nanocellulose could be developed as an excellent solution to these challenges, making nanocellulose extracted from natural algae a very important medical material that is compatible with sustainable development.					
37349573	12	31	theme	excellent	1818:1826	arg1	solution					1828:1835	an excellent solution	1815:1835	an excellent solution to these challenges	1815:1855	These results demonstrate that nanocellulose could be developed as an excellent solution to these challenges, making nanocellulose extracted from natural algae a very important medical material that is compatible with sustainable development.					
37349573	1	32	theme	proper	180:185	arg1	nanotechnology					126:139	Green nanotechnology	120:139	Green nanotechnology	120:139	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	1	32	theme	proper	180:185	arg1	tool					197:200	a more proper and safer tool	173:200	a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals	173:313	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	5	33	theme	analysis	795:802	arg1	peaks					804:808	infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks	751:808	infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks	751:808	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	12	34	theme	natural	1894:1900	arg1	algae					1902:1906	natural algae	1894:1906	natural algae	1894:1906	These results demonstrate that nanocellulose could be developed as an excellent solution to these challenges, making nanocellulose extracted from natural algae a very important medical material that is compatible with sustainable development.					
37349573	7	35	theme	Scanning	930:937	arg1	microscope					948:957	Scanning electron microscope	930:957	Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition	930:1072	Nanocellulose was examined by Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition.					
37349573	7	35	theme	Scanning	930:937	arg1	SEM					960:962	SEM	960:962	SEM	960:962	Nanocellulose was examined by Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition.					
37349573	5	36	theme	same	728:731	arg1	Fourier					733:739	the same Fourier	724:739	the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks	724:808	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	5	36	theme	same	728:731	arg1	cellulose					702:710	reference cellulose	692:710	reference cellulose	692:710	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	3	37	theme	high	429:432	arg1	content					434:440	a high content	427:440	a high content of cellulose	427:453	Algae are abundant in the environment and have a high content of cellulose.					
37349573	8	38	theme	XRD	1140:1142	arg1	analysis					1144:1151	XRD analysis	1140:1151	XRD analysis	1140:1151	The size of nanocellulose in the range of 50 nm is calculated by XRD analysis.					
37349573	2	39	used	used	343:346	arg2	biomass					331:337	The macroalgal biomass	316:337	The macroalgal biomass	316:337	The macroalgal biomass was used for nanocellulose biosynthesis.					
37349573	1	40	theme	safer	191:195	arg1	nanotechnology					126:139	Green nanotechnology	120:139	Green nanotechnology	120:139	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	1	40	theme	safer	191:195	arg1	tool					197:200	a more proper and safer tool	173:200	a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals	173:313	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	0	41	theme	cellulose	16:24	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis of cellulose from Ulva lactuca	0:42	Biosynthesis of cellulose from Ulva lactuca, manufacture of nanocellulose and its application as antimicrobial polymer.					
37349573	0	41	theme	cellulose	16:24	arg1	application					82:92	its application	78:92	its application as antimicrobial polymer	78:117	Biosynthesis of cellulose from Ulva lactuca, manufacture of nanocellulose and its application as antimicrobial polymer.					
37349573	0	41	theme	cellulose	16:24	arg1	manufacture					45:55	manufacture	45:55	manufacture of nanocellulose	45:72	Biosynthesis of cellulose from Ulva lactuca, manufacture of nanocellulose and its application as antimicrobial polymer.					
37349573	9	42	dep	Klebsiella	1270:1279	arg1	pneumonia					1281:1289	Klebsiella pneumonia (ST627)	1270:1297	Klebsiella pneumonia (ST627)	1270:1297	Antibacterial examination of nanocellulose was tested against Gram+ bacteria like Staphylococcus aureus (ATCC6538), Klebsiella pneumonia (ST627), and Gram-negative bacteria such as Escherichia coli (ATCC25922), and coagulase-negative Staphylococci (CoNS) to give 4.06, 4.66, 4.93 and 4.43 cm as respectively.					
37349573	7	43	theme	chemical	1053:1060	arg1	composition					1062:1072	the chemical composition	1049:1072	the chemical composition	1049:1072	Nanocellulose was examined by Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition.					
37349573	5	44	theme	X-Ray	771:775	arg1	XRD					790:792	XRD	790:792	XRD	790:792	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	5	44	theme	X-Ray	771:775	arg1	diffraction					777:787	X-Ray diffraction	771:787	X-Ray diffraction (XRD)	771:793	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	6	45	theme	sulfuric	885:892	arg1	acid					894:897	sulfuric acid	885:897	sulfuric acid	885:897	Nanocellulose was synthesized from extracted cellulose with hydrolysis by sulfuric acid.					
37349573	3	46	theme	cellulose	445:453	arg1	content					434:440	a high content	427:440	a high content of cellulose	427:453	Algae are abundant in the environment and have a high content of cellulose.					
37349573	5	47	theme	diffraction	777:787	arg1	peaks					804:808	infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks	751:808	infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks	751:808	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	1	48	theme	injurious	295:303	arg1	chemicals					305:313	injurious chemicals	295:313	injurious chemicals	295:313	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	7	49	theme	Energy	1008:1013	arg1	EDX					1033:1035	EDX	1033:1035	EDX	1033:1035	Nanocellulose was examined by Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition.					
37349573	7	49	theme	Energy	1008:1013	arg1	X-ray					1026:1030	Energy dispersive X-ray	1008:1030	Energy dispersive X-ray (EDX)	1008:1036	Nanocellulose was examined by Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition.					
37349573	1	50	theme	medical	206:212	arg1	applications					214:225	medical applications	206:225	medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals	206:313	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	10	51	theme	nanocellulose	1594:1606	arg1	MIC					1586:1588	MIC	1586:1588	MIC	1586:1588	Comparing the antibacterial effect of nanocellulose with some antibiotics and estimating minimal Inhibitory Concentration (MIC) of nanocellulose.					
37349573	10	51	theme	nanocellulose	1594:1606	arg1	Concentration					1571:1583	minimal Inhibitory Concentration	1552:1583	minimal Inhibitory Concentration (MIC) of nanocellulose	1552:1606	Comparing the antibacterial effect of nanocellulose with some antibiotics and estimating minimal Inhibitory Concentration (MIC) of nanocellulose.					
37349573	1	52	theme	chemicals	305:313	arg1	avoidance					282:290	avoidance	282:290	avoidance of injurious chemicals	282:313	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	1	52	theme	chemicals	305:313	arg1	toxicity					269:276	low toxicity	265:276	low toxicity	265:276	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	8	53	theme	nanocellulose	1087:1099	arg1	size					1079:1082	The size	1075:1082	The size of nanocellulose in the range of 50 nm	1075:1121	The size of nanocellulose in the range of 50 nm is calculated by XRD analysis.					
37349573	6	54	theme	extracted	846:854	arg1	cellulose					856:864	extracted cellulose	846:864	extracted cellulose	846:864	Nanocellulose was synthesized from extracted cellulose with hydrolysis by sulfuric acid.					
37349573	3	55	from	abundant	390:397	arg1	environment					406:416	the environment	402:416	the environment	402:416	Algae are abundant in the environment and have a high content of cellulose.					
37349573	5	56	dep	infrared	751:758	arg1	FTIR					761:764	FTIR	761:764	FTIR	761:764	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	12	57	with	compatible	1950:1959	arg1	development					1978:1988	sustainable development	1966:1988	sustainable development	1966:1988	These results demonstrate that nanocellulose could be developed as an excellent solution to these challenges, making nanocellulose extracted from natural algae a very important medical material that is compatible with sustainable development.					
37349573	1	58	theme	Green	120:124	arg1	nanotechnology					126:139	Green nanotechnology	120:139	Green nanotechnology	120:139	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	1	58	theme	Green	120:124	arg1	tool					197:200	a more proper and safer tool	173:200	a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals	173:313	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	4	59	theme	consecutive	524:534	arg1	treatments					536:545	consecutive treatments	524:545	consecutive treatments	524:545	In our study, we extracted parent cellulose from Ulva lactuca where consecutive treatments extracted cellulose to obtain an insoluble fraction rich in cellulose.					
37349573	10	60	theme	antibacterial	1477:1489	arg1	effect					1491:1496	the antibacterial effect	1473:1496	the antibacterial effect of nanocellulose with some antibiotics	1473:1535	Comparing the antibacterial effect of nanocellulose with some antibiotics and estimating minimal Inhibitory Concentration (MIC) of nanocellulose.					
37349573	11	61	theme	Candida	1706:1712	arg1	albicans					1714:1721	Candida albicans	1706:1721	Candida albicans	1706:1721	We tested the influence of cellulose and nanocellulose on some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis.					
37349573	1	62	with	reductions	249:258	arg1	avoidance					282:290	avoidance	282:290	avoidance of injurious chemicals	282:313	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	1	62	with	reductions	249:258	arg1	toxicity					269:276	low toxicity	265:276	low toxicity	265:276	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	8	63	from	size	1079:1082	arg1	range					1108:1112	the range	1104:1112	the range of 50 nm	1104:1121	The size of nanocellulose in the range of 50 nm is calculated by XRD analysis.					
37349573	5	64	theme	reference	692:700	arg1	Fourier					733:739	the same Fourier	724:739	the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks	724:808	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	5	64	theme	reference	692:700	arg1	cellulose					702:710	reference cellulose	692:710	reference cellulose	692:710	The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks.					
37349573	11	65	theme	Aspergillus	1686:1696	arg1	flavus					1698:1703	Aspergillus flavus	1686:1703	Aspergillus flavus	1686:1703	We tested the influence of cellulose and nanocellulose on some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis.					
37349573	2	66	theme	macroalgal	320:329	arg1	biomass					331:337	The macroalgal biomass	316:337	The macroalgal biomass	316:337	The macroalgal biomass was used for nanocellulose biosynthesis.					
37349573	1	67	theme	thanks	227:232	arg1	applications					214:225	medical applications	206:225	medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals	206:313	Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals.					
37349573	0	68	theme	nanocellulose	60:72	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis of cellulose from Ulva lactuca	0:42	Biosynthesis of cellulose from Ulva lactuca, manufacture of nanocellulose and its application as antimicrobial polymer.					
37349573	0	68	theme	nanocellulose	60:72	arg1	application					82:92	its application	78:92	its application as antimicrobial polymer	78:117	Biosynthesis of cellulose from Ulva lactuca, manufacture of nanocellulose and its application as antimicrobial polymer.					
37349573	0	68	theme	nanocellulose	60:72	arg1	manufacture					45:55	manufacture	45:55	manufacture of nanocellulose	45:72	Biosynthesis of cellulose from Ulva lactuca, manufacture of nanocellulose and its application as antimicrobial polymer.					
37349573	10	69	with	effect	1491:1496	arg1	antibiotics					1525:1535	some antibiotics	1520:1535	some antibiotics	1520:1535	Comparing the antibacterial effect of nanocellulose with some antibiotics and estimating minimal Inhibitory Concentration (MIC) of nanocellulose.					
37349573	10	70	theme	nanocellulose	1501:1513	arg1	effect					1491:1496	the antibacterial effect	1473:1496	the antibacterial effect of nanocellulose with some antibiotics	1473:1535	Comparing the antibacterial effect of nanocellulose with some antibiotics and estimating minimal Inhibitory Concentration (MIC) of nanocellulose.					
37349573	9	71	theme	coagulase-negative	1369:1386	arg1	CoNS					1403:1406	CoNS	1403:1406	CoNS	1403:1406	Antibacterial examination of nanocellulose was tested against Gram+ bacteria like Staphylococcus aureus (ATCC6538), Klebsiella pneumonia (ST627), and Gram-negative bacteria such as Escherichia coli (ATCC25922), and coagulase-negative Staphylococci (CoNS) to give 4.06, 4.66, 4.93 and 4.43 cm as respectively.					
37349573	9	71	theme	coagulase-negative	1369:1386	arg1	Staphylococci					1388:1400	coagulase-negative Staphylococci	1369:1400	coagulase-negative Staphylococci (CoNS)	1369:1407	Antibacterial examination of nanocellulose was tested against Gram+ bacteria like Staphylococcus aureus (ATCC6538), Klebsiella pneumonia (ST627), and Gram-negative bacteria such as Escherichia coli (ATCC25922), and coagulase-negative Staphylococci (CoNS) to give 4.06, 4.66, 4.93 and 4.43 cm as respectively.					
37349573	11	72	from	influence	1623:1631	arg1	flavus					1698:1703	Aspergillus flavus	1686:1703	Aspergillus flavus	1686:1703	We tested the influence of cellulose and nanocellulose on some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis.					
37349573	11	72	from	influence	1623:1631	arg1	albicans					1714:1721	Candida albicans	1706:1721	Candida albicans	1706:1721	We tested the influence of cellulose and nanocellulose on some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis.					
37349573	11	72	from	influence	1623:1631	arg1	fungi					1672:1676	some fungi	1667:1676	some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis	1667:1745	We tested the influence of cellulose and nanocellulose on some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis.					
37349573	11	72	from	influence	1623:1631	arg1	tropicalis					1736:1745	Candida tropicalis	1728:1745	Candida tropicalis	1728:1745	We tested the influence of cellulose and nanocellulose on some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis.					
37349573	9	73	theme	Gram-negative	1304:1316	arg1	bacteria					1318:1325	Gram-negative bacteria	1304:1325	Gram-negative bacteria such as Escherichia coli (ATCC25922)	1304:1362	Antibacterial examination of nanocellulose was tested against Gram+ bacteria like Staphylococcus aureus (ATCC6538), Klebsiella pneumonia (ST627), and Gram-negative bacteria such as Escherichia coli (ATCC25922), and coagulase-negative Staphylococci (CoNS) to give 4.06, 4.66, 4.93 and 4.43 cm as respectively.					
37349573	9	73	theme	Gram-negative	1304:1316	arg1	ATCC25922					1353:1361	ATCC25922	1353:1361	ATCC25922	1353:1361	Antibacterial examination of nanocellulose was tested against Gram+ bacteria like Staphylococcus aureus (ATCC6538), Klebsiella pneumonia (ST627), and Gram-negative bacteria such as Escherichia coli (ATCC25922), and coagulase-negative Staphylococci (CoNS) to give 4.06, 4.66, 4.93 and 4.43 cm as respectively.					
37349573	9	73	theme	Gram-negative	1304:1316	arg1	coli					1347:1350	Escherichia coli	1335:1350	Escherichia coli	1335:1350	Antibacterial examination of nanocellulose was tested against Gram+ bacteria like Staphylococcus aureus (ATCC6538), Klebsiella pneumonia (ST627), and Gram-negative bacteria such as Escherichia coli (ATCC25922), and coagulase-negative Staphylococci (CoNS) to give 4.06, 4.66, 4.93 and 4.43 cm as respectively.					
35346739	6	0	theme	non-linear	895:904	arg1	relationship					906:917	a non-linear relationship	893:917	a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L)	893:977	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	7	1	theme	substrate	1086:1094	arg1	inoculum					1114:1121	7% inoculum	1111:1121	7% inoculum	1111:1121	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	1	theme	substrate	1086:1094	arg1	concentration					1096:1108	38.8% substrate concentration	1080:1108	38.8% substrate concentration	1080:1108	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	1	theme	substrate	1086:1094	arg1	pH					1124:1125	pH 5.45	1124:1130	pH 5.45	1124:1130	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	2	theme	peel	1394:1397	arg1	"					1404:1404	zero-value waste "Potato peel waste"	1369:1404	zero-value waste "Potato peel waste" through the WtE approach	1369:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	3	theme	38.8	1080:1083	arg1	%					1084:1084	%	1084:1084	%	1084:1084	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	4	theme	WtE	1418:1420	arg1	approach					1422:1429	the WtE approach	1414:1429	the WtE approach	1414:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	2	5	theme	isolated	279:286	arg1	amyloliquefaciens					369:385	B. amyloliquefaciens	366:385	B. amyloliquefaciens	366:385	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	5	theme	isolated	279:286	arg1	capable					478:484	capable	478:484	capable	478:484	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	5	theme	isolated	279:286	arg1	strain					358:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain	269:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain	269:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	1	6	theme	wastes	174:179	arg1	wastes					174:179	the zero-value wastes	159:179	the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach	159:266	Potato peel waste is one of the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach.					
35346739	1	6	theme	wastes	174:179	arg1	one					152:154	one	152:154	one	152:154	Potato peel waste is one of the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach.					
35346739	7	7	theme	zero-value	1369:1378	arg1	"					1404:1404	zero-value waste "Potato peel waste"	1369:1404	zero-value waste "Potato peel waste" through the WtE approach	1369:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	8	theme	Optimal	1046:1052	arg1	variables					1067:1075	Optimal fermentation variables	1046:1075	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45	1046:1130	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	6	9	theme	R2	1035:1036	arg1	values					1038:1043	97.50 adj. R2 values	1024:1043	97.50 adj. R2 values	1024:1043	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	7	10	from	production	1166:1175	arg1	55.27 h					1180:1186	55.27 h	1180:1186	55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1180:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	10	from	production	1166:1175	arg1	27 °C.					1191:1196	27 °C.	1191:1196	27 °C.	1191:1196	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	11	theme	sustainable	1330:1340	arg1	production					1353:1362	sustainable bioethanol production	1330:1362	sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1330:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	4	12	theme	composite	692:700	arg1	design					702:707	the central composite design	680:707	the central composite design of response surface methodology	680:739	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	8	13	with	strains	1521:1527	arg1	capabilities					1574:1585	the starch-hydrolyzing and fermentation capabilities	1534:1585	capabilities	1574:1585	Moreover, the present study introduces the promising, unexplored extremophilic microbial strains with the starch-hydrolyzing and fermentation capabilities to bioethanol biorefinery.					
35346739	6	14	theme	variables	935:943	arg1	relationship					906:917	a non-linear relationship	893:917	a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L)	893:977	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	7	15	theme	region	1320:1325	arg1	microbes					1294:1301	newly isolated, indigenous extremophilic microbes	1253:1301	newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1253:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	4	16	dep	isolated	578:585	arg1	characterized					603:615	characterized	603:615	characterized	603:615	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	1	17	theme	Potato	131:136	arg1	waste					143:147	Potato peel waste	131:147	Potato peel waste	131:147	Potato peel waste is one of the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach.					
35346739	7	18	from	27 °C.	1191:1196	arg1	55.27 h					1180:1186	55.27 h	1180:1186	55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1180:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	18	from	27 °C.	1191:1196	arg1	production					1166:1175	bioethanol production	1155:1175	bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1155:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	19	theme	isolated	1259:1266	arg1	microbes					1294:1301	newly isolated, indigenous extremophilic microbes	1253:1301	newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1253:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	0	20	theme	bioethanol	55:64	arg1	production					66:75	bioethanol production	55:75	bioethanol production using indigenous microbes of Himachal Pradesh, India	55:128	A sustainable approach of turning potato waste towards bioethanol production using indigenous microbes of Himachal Pradesh, India.					
35346739	6	21	theme	desirability	993:1004	arg1	function					1006:1013	a 99.11% desirability function	984:1013	a 99.11% desirability function (R2)	984:1018	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	6	21	theme	desirability	993:1004	arg1	R2					1016:1017	R2	1016:1017	R2	1016:1017	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	7	22	from	"	1404:1404	arg1	region					1320:1325	The Himalayan region	1306:1325	The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1306:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	22	from	"	1404:1404	arg1	production					1353:1362	sustainable bioethanol production	1330:1362	sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1330:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	6	23	theme	99.11	986:990	arg1	%					991:991	%	991:991	%	991:991	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	2	24	dep	identified	333:342	arg1	molecular					323:331	molecular	323:331	molecular	323:331	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	7	25	used	utilized	1142:1149	arg2	variables					1067:1075	Optimal fermentation variables	1046:1075	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45	1046:1130	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	26	from	region	1320:1325	arg1	"					1404:1404	zero-value waste "Potato peel waste"	1369:1404	zero-value waste "Potato peel waste" through the WtE approach	1369:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	26	from	region	1320:1325	arg1	production					1353:1362	sustainable bioethanol production	1330:1362	sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1330:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	8	27	theme	extremophilic	1497:1509	arg1	strains					1521:1527	the promising, unexplored extremophilic microbial strains	1471:1527	the promising, unexplored extremophilic microbial strains with the starch-hydrolyzing and fermentation capabilities to bioethanol biorefinery	1471:1611	Moreover, the present study introduces the promising, unexplored extremophilic microbial strains with the starch-hydrolyzing and fermentation capabilities to bioethanol biorefinery.					
35346739	4	28	theme	surface	721:727	arg1	methodology					729:739	response surface methodology	712:739	response surface methodology	712:739	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	1	29	theme	WtE	254:256	arg1	approach					259:266	the Waste to Energy (WtE) approach	233:266	the Waste to Energy (WtE) approach	233:266	Potato peel waste is one of the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach.					
35346739	7	30	theme	extremophilic	1280:1292	arg1	microbes					1294:1301	newly isolated, indigenous extremophilic microbes	1253:1301	newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1253:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	2	31	theme	12.06 g/L	423:431	arg1	sugars					442:447	12.06 g/L reducing sugars	423:447	12.06 g/L reducing sugars	423:447	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	31	theme	12.06 g/L	423:431	arg1	hydrolysis					411:420	promising starch hydrolysis	394:420	promising starch hydrolysis (12.06 g/L reducing sugars)	394:448	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	7	32	from	production	1353:1362	arg1	microbes					1294:1301	newly isolated, indigenous extremophilic microbes	1253:1301	newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1253:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	6	33	theme	97.50	1024:1028	arg1	values					1038:1043	97.50 adj. R2 values	1024:1043	97.50 adj. R2 values	1024:1043	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	2	34	theme	promising	394:402	arg1	sugars					442:447	12.06 g/L reducing sugars	423:447	12.06 g/L reducing sugars	423:447	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	34	theme	promising	394:402	arg1	hydrolysis					411:420	promising starch hydrolysis	394:420	promising starch hydrolysis (12.06 g/L reducing sugars)	394:448	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	7	35	theme	%	1112:1112	arg1	inoculum					1114:1121	7% inoculum	1111:1121	7% inoculum	1111:1121	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	35	theme	%	1112:1112	arg1	concentration					1096:1108	38.8% substrate concentration	1080:1108	38.8% substrate concentration	1080:1108	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	1	36	with	wastes	174:179	arg1	potential					190:198	the potential	186:198	the potential of bioethanol production through the Waste to Energy (WtE) approach	186:266	Potato peel waste is one of the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach.					
35346739	4	37	theme	input	781:785	arg1	variables					787:795	input variables	781:795	input variables	781:795	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	2	38	theme	characterized	304:316	arg1	amyloliquefaciens					369:385	B. amyloliquefaciens	366:385	B. amyloliquefaciens	366:385	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	38	theme	characterized	304:316	arg1	capable					478:484	capable	478:484	capable	478:484	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	38	theme	characterized	304:316	arg1	strain					358:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain	269:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain	269:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	1	39	theme	bioethanol	203:212	arg1	production					214:223	bioethanol production	203:223	bioethanol production through the Waste to Energy (WtE) approach	203:266	Potato peel waste is one of the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach.					
35346739	8	40	theme	bioethanol	1590:1599	arg1	biorefinery					1601:1611	bioethanol biorefinery	1590:1611	bioethanol biorefinery	1590:1611	Moreover, the present study introduces the promising, unexplored extremophilic microbial strains with the starch-hydrolyzing and fermentation capabilities to bioethanol biorefinery.					
35346739	6	41	theme	fermentation	922:933	arg1	variables					935:943	fermentation variables	922:943	fermentation variables	922:943	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	7	42	from	microbes	1294:1301	arg1	production					1353:1362	sustainable bioethanol production	1330:1362	sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1330:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	43	theme	present	1211:1217	arg1	study					1219:1223	the present study	1207:1223	the present study	1207:1223	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	44	theme	concentration	1096:1108	arg1	variables					1067:1075	Optimal fermentation variables	1046:1075	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45	1046:1130	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	2	45	theme	identified	333:342	arg1	amyloliquefaciens					369:385	B. amyloliquefaciens	366:385	B. amyloliquefaciens	366:385	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	45	theme	identified	333:342	arg1	capable					478:484	capable	478:484	capable	478:484	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	45	theme	identified	333:342	arg1	strain					358:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain	269:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain	269:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	1	46	theme	Waste	237:241	arg1	approach					259:266	the Waste to Energy (WtE) approach	233:266	the Waste to Energy (WtE) approach	233:266	Potato peel waste is one of the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach.					
35346739	7	47	theme	%	1084:1084	arg1	inoculum					1114:1121	7% inoculum	1111:1121	7% inoculum	1111:1121	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	47	theme	%	1084:1084	arg1	concentration					1096:1108	38.8% substrate concentration	1080:1108	38.8% substrate concentration	1080:1108	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	47	theme	%	1084:1084	arg1	pH					1124:1125	pH 5.45	1124:1130	pH 5.45	1124:1130	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	1	48	theme	Energy	246:251	arg1	approach					259:266	the Waste to Energy (WtE) approach	233:266	the Waste to Energy (WtE) approach	233:266	Potato peel waste is one of the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach.					
35346739	5	49	theme	ethanol	843:849	arg1	production					851:860	The ethanol production	839:860	The ethanol production by Acinetobacter sp	839:880	The ethanol production by Acinetobacter sp.					
35346739	4	50	theme	output	822:827	arg1	variable					829:836	the output variable	818:836	the output variable	818:836	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	4	50	theme	output	822:827	arg1	variables					768:776	the fermentation variables	751:776	the fermentation variables as input variables and ethanol yield	751:813	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	7	51	theme	waste	1399:1403	arg1	"					1404:1404	zero-value waste "Potato peel waste"	1369:1404	zero-value waste "Potato peel waste" through the WtE approach	1369:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	52	theme	fermentation	1054:1065	arg1	variables					1067:1075	Optimal fermentation variables	1046:1075	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45	1046:1130	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	1	53	theme	zero-value	163:172	arg1	wastes					174:179	the zero-value wastes	159:179	the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach	159:266	Potato peel waste is one of the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach.					
35346739	7	54	theme	Potato	1387:1392	arg1	"					1404:1404	zero-value waste "Potato peel waste"	1369:1404	zero-value waste "Potato peel waste" through the WtE approach	1369:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	0	55	theme	India	124:128	arg1	microbes					94:101	indigenous microbes	83:101	indigenous microbes of Himachal Pradesh, India	83:128	A sustainable approach of turning potato waste towards bioethanol production using indigenous microbes of Himachal Pradesh, India.					
35346739	3	56	theme	ethanol	529:535	arg1	production					537:546	The ethanol production	525:546	The ethanol production by Acinetobacter sp	525:566	The ethanol production by Acinetobacter sp.					
35346739	3	57	theme	Acinetobacter	551:563	arg1	sp					565:566	Acinetobacter sp	551:566	Acinetobacter sp	551:566	The ethanol production by Acinetobacter sp.					
35346739	7	58	theme	waste	1380:1384	arg1	"					1404:1404	zero-value waste "Potato peel waste"	1369:1404	zero-value waste "Potato peel waste" through the WtE approach	1369:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	5	59	theme	Acinetobacter	865:877	arg1	sp					879:880	Acinetobacter sp	865:880	Acinetobacter sp	865:880	The ethanol production by Acinetobacter sp.					
35346739	6	60	theme	ethanol	954:960	arg1	5.83 g/L					969:976	5.83 g/L	969:976	5.83 g/L	969:976	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	6	60	theme	ethanol	954:960	arg1	yield					962:966	the ethanol yield	950:966	the ethanol yield (5.83 g/L)	950:977	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	7	61	theme	bioethanol	1342:1351	arg1	production					1353:1362	sustainable bioethanol production	1330:1362	sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1330:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	4	62	theme	central	684:690	arg1	design					702:707	the central composite design	680:707	the central composite design of response surface methodology	680:739	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	8	63	theme	present	1446:1452	arg1	study					1454:1458	the present study	1442:1458	the present study	1442:1458	Moreover, the present study introduces the promising, unexplored extremophilic microbial strains with the starch-hydrolyzing and fermentation capabilities to bioethanol biorefinery.					
35346739	1	64	theme	peel	138:141	arg1	waste					143:147	Potato peel waste	131:147	Potato peel waste	131:147	Potato peel waste is one of the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach.					
35346739	1	65	dep	approach	259:266	arg1	to					243:244	to	243:244	to	243:244	Potato peel waste is one of the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach.					
35346739	7	66	theme	Himalayan	1310:1318	arg1	region					1320:1325	The Himalayan region	1306:1325	The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1306:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	0	67	theme	indigenous	83:92	arg1	microbes					94:101	indigenous microbes	83:101	indigenous microbes of Himachal Pradesh, India	83:128	A sustainable approach of turning potato waste towards bioethanol production using indigenous microbes of Himachal Pradesh, India.					
35346739	6	68	theme	%	991:991	arg1	function					1006:1013	a 99.11% desirability function	984:1013	a 99.11% desirability function (R2)	984:1018	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	6	68	theme	%	991:991	arg1	R2					1016:1017	R2	1016:1017	R2	1016:1017	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	8	69	theme	promising	1475:1483	arg1	strains					1521:1527	the promising, unexplored extremophilic microbial strains	1471:1527	the promising, unexplored extremophilic microbial strains with the starch-hydrolyzing and fermentation capabilities to bioethanol biorefinery	1471:1611	Moreover, the present study introduces the promising, unexplored extremophilic microbial strains with the starch-hydrolyzing and fermentation capabilities to bioethanol biorefinery.					
35346739	7	70	theme	bioethanol	1155:1164	arg1	production					1166:1175	bioethanol production	1155:1175	bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1155:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	7	71	theme	microbes	1294:1301	arg1	efficiency					1239:1248	the efficiency	1235:1248	the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1235:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	2	72	theme	working	489:495	arg1	capable					478:484	capable	478:484	capable	478:484	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	72	theme	working	489:495	arg1	strain					358:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain	269:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain	269:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	8	73	theme	microbial	1511:1519	arg1	strains					1521:1527	the promising, unexplored extremophilic microbial strains	1471:1527	the promising, unexplored extremophilic microbial strains with the starch-hydrolyzing and fermentation capabilities to bioethanol biorefinery	1471:1611	Moreover, the present study introduces the promising, unexplored extremophilic microbial strains with the starch-hydrolyzing and fermentation capabilities to bioethanol biorefinery.					
35346739	4	74	theme	fermentation	755:766	arg1	variable					829:836	the output variable	818:836	the output variable	818:836	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	4	74	theme	fermentation	755:766	arg1	variables					768:776	the fermentation variables	751:776	the fermentation variables as input variables and ethanol yield	751:813	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	0	75	theme	sustainable	2:12	arg1	approach					14:21	A sustainable approach	0:21	A sustainable approach of turning potato waste towards bioethanol production using indigenous microbes of Himachal Pradesh, India.	0:129	A sustainable approach of turning potato waste towards bioethanol production using indigenous microbes of Himachal Pradesh, India.					
35346739	2	76	theme	reducing	433:440	arg1	sugars					442:447	12.06 g/L reducing sugars	423:447	12.06 g/L reducing sugars	423:447	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	76	theme	reducing	433:440	arg1	hydrolysis					411:420	promising starch hydrolysis	394:420	promising starch hydrolysis (12.06 g/L reducing sugars)	394:448	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	4	77	theme	response	712:719	arg1	methodology					729:739	response surface methodology	712:739	response surface methodology	712:739	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	6	78	theme	adj.	1030:1033	arg1	values					1038:1043	97.50 adj. R2 values	1024:1043	97.50 adj. R2 values	1024:1043	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	8	79	theme	starch-hydrolyzing	1538:1555	arg1	capabilities					1574:1585	the starch-hydrolyzing and fermentation capabilities	1534:1585	capabilities	1574:1585	Moreover, the present study introduces the promising, unexplored extremophilic microbial strains with the starch-hydrolyzing and fermentation capabilities to bioethanol biorefinery.					
35346739	4	80	theme	methodology	729:739	arg1	design					702:707	the central composite design	680:707	the central composite design of response surface methodology	680:739	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	2	81	theme	starch	404:409	arg1	sugars					442:447	12.06 g/L reducing sugars	423:447	12.06 g/L reducing sugars	423:447	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	81	theme	starch	404:409	arg1	hydrolysis					411:420	promising starch hydrolysis	394:420	promising starch hydrolysis (12.06 g/L reducing sugars)	394:448	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	0	82	theme	potato	34:39	arg1	waste					41:45	potato waste	34:45	potato waste	34:45	A sustainable approach of turning potato waste towards bioethanol production using indigenous microbes of Himachal Pradesh, India.					
35346739	2	83	theme	acid	455:458	arg1	hydrolysis					460:469	acid hydrolysis	455:469	acid hydrolysis	455:469	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	8	84	theme	fermentation	1561:1572	arg1	capabilities					1574:1585	the starch-hydrolyzing and fermentation capabilities	1534:1585	capabilities	1574:1585	Moreover, the present study introduces the promising, unexplored extremophilic microbial strains with the starch-hydrolyzing and fermentation capabilities to bioethanol biorefinery.					
35346739	7	85	dep	27 °C.	1191:1196	arg1	evaluated					1225:1233	evaluated	1225:1233	evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach	1225:1429	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	4	86	theme	ethanol	801:807	arg1	yield					809:813	ethanol yield	801:813	ethanol yield	801:813	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	7	87	theme	7	1111:1111	arg1	%					1112:1112	%	1112:1112	%	1112:1112	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	6	88	with	relationship	906:917	arg1	5.83 g/L					969:976	5.83 g/L	969:976	5.83 g/L	969:976	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	6	88	with	relationship	906:917	arg1	yield					962:966	the ethanol yield	950:966	the ethanol yield (5.83 g/L)	950:977	showcased a non-linear relationship of fermentation variables with the ethanol yield (5.83 g/L) with a 99.11% desirability function (R2) and 97.50 adj. R2 values.					
35346739	8	89	dep	promising	1475:1483	arg1	unexplored					1486:1495	unexplored	1486:1495	unexplored	1486:1495	Moreover, the present study introduces the promising, unexplored extremophilic microbial strains with the starch-hydrolyzing and fermentation capabilities to bioethanol biorefinery.					
35346739	4	90	dep	modelled	649:656	arg1	molecular					618:626	a newly isolated, phenotypically characterized, molecular	570:626	a newly isolated, phenotypically characterized, molecular identified	570:637	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	4	91	theme	isolated	578:585	arg1	molecular					618:626	a newly isolated, phenotypically characterized, molecular	570:626	a newly isolated, phenotypically characterized, molecular identified	570:637	(a newly isolated, phenotypically characterized, molecular identified) has been modelled and optimized through the central composite design of response surface methodology by taking the fermentation variables as input variables and ethanol yield as the output variable.					
35346739	1	92	theme	production	214:223	arg1	potential					190:198	the potential	186:198	the potential of bioethanol production through the Waste to Energy (WtE) approach	186:266	Potato peel waste is one of the zero-value wastes with the potential of bioethanol production through the Waste to Energy (WtE) approach.					
35346739	7	93	dep	isolated	1259:1266	arg1	indigenous					1269:1278	indigenous	1269:1278	indigenous	1269:1278	Optimal fermentation variables of 38.8% substrate concentration, 7% inoculum, pH 5.45 have been utilized for bioethanol production in 55.27 h at 27 °C. Overall, the present study evaluated the efficiency of newly isolated, indigenous extremophilic microbes of The Himalayan region in sustainable bioethanol production from zero-value waste "Potato peel waste" through the WtE approach.					
35346739	2	94	theme	high-altitude	344:356	arg1	amyloliquefaciens					369:385	B. amyloliquefaciens	366:385	B. amyloliquefaciens	366:385	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	94	theme	high-altitude	344:356	arg1	capable					478:484	capable	478:484	capable	478:484	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
35346739	2	94	theme	high-altitude	344:356	arg1	strain					358:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain	269:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain	269:363	The newly isolated, phenotypically characterized, and molecular identified high-altitude strain, B. amyloliquefaciens, shown promising starch hydrolysis (12.06 g/L reducing sugars) over acid hydrolysis and is capable of working at 30-50 °C and pH 6.0-8.0.					
36298752	16	0	theme	genus	2435:2439	arg1	Dorea					2441:2445	the genus Dorea	2431:2445	the genus Dorea	2431:2445	On the other hand, Bello, the only mosquito population classified as susceptible to lambda-cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females.					
36298752	18	1	theme	alginate	2912:2919	arg1	synthesis					2921:2929	alginate synthesis	2912:2929	alginate synthesis	2912:2929	Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis.					
36298752	11	2	theme	infection	1572:1580	arg1	rates					1582:1586	high ZIKV infection rates	1562:1586	high ZIKV infection rates	1562:1586	RESULTS We found high ZIKV infection rates in Ae.					
36298752	2	3	from	outbreak	287:294	arg1	America					305:311	America	305:311	America	305:311	In 2015-16, Zika virus (ZIKV) had an outbreak in South America associated with prenatal microcephaly and Guillain-Barré syndrome.					
36298752	18	4	theme	insecticides	2801:2812	arg1	degradation					2786:2796	the degradation	2782:2796	the degradation of insecticides	2782:2812	Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis.					
36298752	17	5	theme	related	2552:2558	arg1	functions					2542:2550	functions	2542:2550	functions related to phenazine production, potentially associated with infection control,	2542:2630	The Serratia genus presented significantly decreased functions related to phenazine production, potentially associated with infection control, and control mechanism functions for host defense and quorum sensing.					
36298752	16	6	theme	other	2202:2206	arg1	hand					2208:2211	the other hand	2198:2211	the other hand	2198:2211	On the other hand, Bello, the only mosquito population classified as susceptible to lambda-cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females.					
36298752	11	7	theme	high	1562:1565	arg1	rates					1582:1586	high ZIKV infection rates	1562:1586	high ZIKV infection rates	1562:1586	RESULTS We found high ZIKV infection rates in Ae.					
36298752	20	8	theme	Colombian	3044:3052	arg1	populations					3054:3064	aegypti Colombian populations	3036:3064	aegypti Colombian populations	3036:3064	aegypti Colombian populations.					
36298752	8	9	theme	ZIKV	1247:1250	arg1	infection					1252:1260	natural ZIKV infection	1239:1260	natural ZIKV infection	1239:1260	These analyses were done under the condition of natural ZIKV infection and resistance to lambda-cyhalothrin, alone and in combination.					
36298752	17	10	theme	infection	2613:2621	arg1	control					2623:2629	infection control	2613:2629	infection control	2613:2629	The Serratia genus presented significantly decreased functions related to phenazine production, potentially associated with infection control, and control mechanism functions for host defense and quorum sensing.					
36298752	15	11	theme	intestinal	2172:2181	arg1	infections					2183:2192	human intestinal infections	2166:2192	human intestinal infections	2166:2192	We hypothesize that it is a vehicle for virus entry, as it is in human intestinal infections.					
36298752	4	12	theme	COVID-19	666:673	arg1	pandemic					675:682	the COVID-19 pandemic	662:682	the COVID-19 pandemic	662:682	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	10	13	theme	pyrethroid	1437:1446	arg1	lambda-cyhalothrin					1448:1465	the pyrethroid lambda-cyhalothrin	1433:1465	the pyrethroid lambda-cyhalothrin	1433:1465	We also measured the susceptibility to the pyrethroid lambda-cyhalothrin and evaluated the presence of the V1016I mutation in the sodium channel gene.					
36298752	1	14	theme	several	191:197	arg1	Zika					227:230	Zika	227:230	Zika	227:230	INTRODUCTION Aedes aegypti is the vector of several arboviruses such as dengue, Zika, and chikungunya.					
36298752	1	14	theme	several	191:197	arg1	chikungunya					237:247	chikungunya	237:247	chikungunya	237:247	INTRODUCTION Aedes aegypti is the vector of several arboviruses such as dengue, Zika, and chikungunya.					
36298752	1	14	theme	several	191:197	arg1	arboviruses					199:209	several arboviruses	191:209	several arboviruses such as dengue, Zika, and chikungunya	191:247	INTRODUCTION Aedes aegypti is the vector of several arboviruses such as dengue, Zika, and chikungunya.					
36298752	1	14	theme	several	191:197	arg1	dengue					219:224	dengue	219:224	dengue	219:224	INTRODUCTION Aedes aegypti is the vector of several arboviruses such as dengue, Zika, and chikungunya.					
36298752	16	15	theme	mucin	2339:2343	arg1	functions					2357:2365	mucin degradation functions	2339:2365	mucin degradation functions in the intestine	2339:2382	On the other hand, Bello, the only mosquito population classified as susceptible to lambda-cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females.					
36298752	7	16	theme	aegypti	1076:1082	arg1	populations					1084:1094	aegypti populations	1076:1094	aegypti populations	1076:1094	aegypti populations were analyzed using DADA2 Pipeline, and their functions were predicted with PICRUSt2 analysis.					
36298752	4	17	from	limitations	622:632	arg1	control					583:589	vector control	576:589	vector control	576:589	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	17	from	limitations	622:632	arg1	management					644:653	dengue management	637:653	dengue management during the COVID-19 pandemic	637:682	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	10	18	from	presence	1485:1492	arg1	gene					1539:1542	the sodium channel gene	1520:1542	the sodium channel gene	1520:1542	We also measured the susceptibility to the pyrethroid lambda-cyhalothrin and evaluated the presence of the V1016I mutation in the sodium channel gene.					
36298752	17	19	theme	quorum	2685:2690	arg1	sensing					2692:2698	quorum sensing	2685:2698	quorum sensing	2685:2698	The Serratia genus presented significantly decreased functions related to phenazine production, potentially associated with infection control, and control mechanism functions for host defense and quorum sensing.					
36298752	0	20	from	Infection	70:78	arg1	Populations					134:144	Colombian Aedes aegypti Populations	110:144	Colombian Aedes aegypti Populations	110:144	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	20	21	theme	aegypti	3036:3042	arg1	populations					3054:3064	aegypti Colombian populations	3036:3064	aegypti Colombian populations	3036:3064	aegypti Colombian populations.					
36298752	4	22	theme	dengue	637:642	arg1	management					644:653	dengue management	637:653	dengue management during the COVID-19 pandemic	637:682	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	3	23	with	diversity	457:465	arg1	vector					497:502	the vector	493:502	the vector	493:502	This mosquito's viral transmission is influenced by microbiota abundance and diversity and its interactions with the vector.					
36298752	14	24	theme	infection	1890:1898	arg1	state					1881:1885	a state	1879:1885	a state of infection with ZIKV and lambda-cyhalothrin resistance	1879:1942	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	4	25	theme	effective	697:705	arg1	treatment					707:715	effective treatment	697:715	effective treatment	697:715	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	12	26	theme	Colombian	1616:1624	arg1	Honda					1684:1688	Honda	1684:1688	Honda	1684:1688	aegypti females from Colombian rural municipalities with deficient water supply, such as Honda with 63.6%.					
36298752	12	26	theme	Colombian	1616:1624	arg1	municipalities					1632:1645	Colombian rural municipalities	1616:1645	Colombian rural municipalities	1616:1645	aegypti females from Colombian rural municipalities with deficient water supply, such as Honda with 63.6%.					
36298752	17	27	theme	control	2636:2642	arg1	functions					2654:2662	control mechanism functions	2636:2662	control mechanism functions	2636:2662	The Serratia genus presented significantly decreased functions related to phenazine production, potentially associated with infection control, and control mechanism functions for host defense and quorum sensing.					
36298752	5	28	theme	transmission	933:944	arg1	surveillance					917:928	surveillance	917:928	surveillance of transmission and the risk of outbreaks of these diseases at the local level	917:1007	Its study is fundamental because it generates knowledge for surveillance of transmission and the risk of outbreaks of these diseases at the local level.					
36298752	6	29	theme	Midgut	1018:1023	arg1	compositions					1035:1046	Midgut bacterial compositions	1018:1046	Midgut bacterial compositions of females of Colombian Ae	1018:1073	METHODS Midgut bacterial compositions of females of Colombian Ae.					
36298752	18	30	with	synthesis	2921:2929	arg1	system					2886:2891	a two-component system	2870:2891	a two-component system	2870:2891	Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis.					
36298752	13	31	with	infection	1725:1733	arg1	arbovirus					1743:1751	arbovirus	1743:1751	arbovirus	1743:1751	In the face of natural infection with an arbovirus such as Zika, the diversity between an infective and non-infective form was significantly different.					
36298752	0	32	theme	Midgut	15:20	arg1	Bacteria					22:29	Midgut Bacteria	15:29	Midgut Bacteria	15:29	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	1	33	theme	Aedes	160:164	arg1	vector					181:186	the vector	177:186	the vector of several arboviruses such as dengue, Zika, and chikungunya	177:247	INTRODUCTION Aedes aegypti is the vector of several arboviruses such as dengue, Zika, and chikungunya.					
36298752	1	33	theme	Aedes	160:164	arg1	INTRODUCTION					147:158	INTRODUCTION	147:158	INTRODUCTION Aedes aegypti	147:172	INTRODUCTION Aedes aegypti is the vector of several arboviruses such as dengue, Zika, and chikungunya.					
36298752	17	34	theme	host	2668:2671	arg1	defense					2673:2679	host defense	2668:2679	host defense	2668:2679	The Serratia genus presented significantly decreased functions related to phenazine production, potentially associated with infection control, and control mechanism functions for host defense and quorum sensing.					
36298752	0	35	dep	Aedes	120:124	arg1	aegypti					126:132	aegypti	126:132	aegypti	126:132	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	13	36	theme	infection	1725:1733	arg1	face					1709:1712	the face	1705:1712	the face of natural infection with an arbovirus such as Zika	1705:1764	In the face of natural infection with an arbovirus such as Zika, the diversity between an infective and non-infective form was significantly different.					
36298752	17	37	theme	phenazine	2563:2571	arg1	production					2573:2582	phenazine production	2563:2582	phenazine production	2563:2582	The Serratia genus presented significantly decreased functions related to phenazine production, potentially associated with infection control, and control mechanism functions for host defense and quorum sensing.					
36298752	0	38	theme	Insecticide	84:94	arg1	Resistance					96:105	Insecticide Resistance	84:105	Insecticide Resistance	84:105	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	18	39	with	metabolism	2836:2845	arg1	system					2886:2891	a two-component system	2870:2891	a two-component system	2870:2891	Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis.					
36298752	18	40	with	pumps	2901:2905	arg1	system					2886:2891	a two-component system	2870:2891	a two-component system	2870:2891	Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis.					
36298752	4	41	from	failure	565:571	arg1	control					583:589	vector control	576:589	vector control	576:589	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	41	from	failure	565:571	arg1	management					644:653	dengue management	637:653	dengue management during the COVID-19 pandemic	637:682	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	5	42	theme	diseases	981:988	arg1	outbreaks					962:970	outbreaks	962:970	outbreaks of these diseases	962:988	Its study is fundamental because it generates knowledge for surveillance of transmission and the risk of outbreaks of these diseases at the local level.					
36298752	10	43	theme	V1016I	1501:1506	arg1	mutation					1508:1515	the V1016I mutation	1497:1515	the V1016I mutation	1497:1515	We also measured the susceptibility to the pyrethroid lambda-cyhalothrin and evaluated the presence of the V1016I mutation in the sodium channel gene.					
36298752	11	44	dep	RESULTS	1545:1551	arg1	found					1556:1560	found	1556:1560	found high ZIKV infection rates in Ae	1556:1592	RESULTS We found high ZIKV infection rates in Ae.					
36298752	16	45	theme	only	2225:2228	arg1	population					2239:2248	the only mosquito population	2221:2248	the only mosquito population classified as susceptible to lambda-cyhalothrin	2221:2296	On the other hand, Bello, the only mosquito population classified as susceptible to lambda-cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females.					
36298752	16	45	theme	only	2225:2228	arg1	Bello					2214:2218	Bello	2214:2218	Bello	2214:2218	On the other hand, Bello, the only mosquito population classified as susceptible to lambda-cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females.					
36298752	2	46	theme	Guillain-Barré	355:368	arg1	syndrome					370:377	Guillain-Barré syndrome	355:377	Guillain-Barré syndrome	355:377	In 2015-16, Zika virus (ZIKV) had an outbreak in South America associated with prenatal microcephaly and Guillain-Barré syndrome.					
36298752	14	47	theme	insecticides	2087:2098	arg1	pathogenicity					2024:2036	pathogenicity	2024:2036	pathogenicity	2024:2036	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	14	47	theme	insecticides	2087:2098	arg1	resistance					2053:2062	antimicrobial resistance	2039:2062	antimicrobial resistance	2039:2062	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	14	47	theme	insecticides	2087:2098	arg1	bioremediation					2069:2082	bioremediation	2069:2082	bioremediation of insecticides	2069:2098	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	6	48	theme	Colombian	1062:1070	arg1	Ae					1072:1073	Colombian Ae	1062:1073	Colombian Ae	1062:1073	METHODS Midgut bacterial compositions of females of Colombian Ae.					
36298752	16	49	theme	degradation	2345:2355	arg1	functions					2357:2365	mucin degradation functions	2339:2365	mucin degradation functions in the intestine	2339:2382	On the other hand, Bello, the only mosquito population classified as susceptible to lambda-cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females.					
36298752	10	50	attach	presence	1485:1492	arg1	gene					1539:1542	the sodium channel gene	1520:1542	the sodium channel gene	1520:1542	We also measured the susceptibility to the pyrethroid lambda-cyhalothrin and evaluated the presence of the V1016I mutation in the sodium channel gene.					
36298752	10	50	attach	presence	1485:1492	arg2	mutation					1508:1515	the V1016I mutation	1497:1515	the V1016I mutation	1497:1515	We also measured the susceptibility to the pyrethroid lambda-cyhalothrin and evaluated the presence of the V1016I mutation in the sodium channel gene.					
36298752	7	51	theme	PICRUSt2	1172:1179	arg1	analysis					1181:1188	PICRUSt2 analysis	1172:1188	PICRUSt2 analysis	1172:1188	aegypti populations were analyzed using DADA2 Pipeline, and their functions were predicted with PICRUSt2 analysis.					
36298752	21	52	theme	dysbiosis	3148:3156	arg1	phenome					3137:3143	a phenome	3135:3143	a phenome of dysbiosis in field Ae	3135:3168	The condition of resistance to lambda-cyhalothrin could be inducing a phenome of dysbiosis in field Ae.					
36298752	6	53	theme	females	1051:1057	arg1	compositions					1035:1046	Midgut bacterial compositions	1018:1046	Midgut bacterial compositions of females of Colombian Ae	1018:1073	METHODS Midgut bacterial compositions of females of Colombian Ae.					
36298752	4	54	theme	arboviruses	552:562	arg1	cocirculation					523:535	cocirculation	523:535	cocirculation of these three arboviruses	523:562	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	10	55	theme	sodium	1524:1529	arg1	gene					1539:1542	the sodium channel gene	1520:1542	the sodium channel gene	1520:1542	We also measured the susceptibility to the pyrethroid lambda-cyhalothrin and evaluated the presence of the V1016I mutation in the sodium channel gene.					
36298752	9	56	theme	females	1385:1391	arg1	percentage					1357:1366	the percentage	1353:1366	the percentage of ZIKV-infected females	1353:1391	One-step RT-PCR determined the percentage of ZIKV-infected females.					
36298752	12	57	from	municipalities	1632:1645	arg1	females					1603:1609	aegypti females	1595:1609	aegypti females from Colombian rural municipalities with deficient water supply, such as Honda with 63.6%.	1595:1700	aegypti females from Colombian rural municipalities with deficient water supply, such as Honda with 63.6%.					
36298752	15	58	theme	virus	2141:2145	arg1	entry					2147:2151	virus entry	2141:2151	virus entry	2141:2151	We hypothesize that it is a vehicle for virus entry, as it is in human intestinal infections.					
36298752	14	59	theme	bioremediation	2069:2082	arg1	functions					2011:2019	functions	2011:2019	functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides	2011:2098	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	4	60	from	conditions	509:518	arg1	control					583:589	vector control	576:589	vector control	576:589	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	60	from	conditions	509:518	arg1	management					644:653	dengue management	637:653	dengue management during the COVID-19 pandemic	637:682	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	2	61	contain	had	280:282	arg1	virus					267:271	Zika virus	262:271	Zika virus (ZIKV)	262:278	In 2015-16, Zika virus (ZIKV) had an outbreak in South America associated with prenatal microcephaly and Guillain-Barré syndrome.					
36298752	2	61	contain	had	280:282	arg2	outbreak					287:294	an outbreak	284:294	an outbreak in South America associated with prenatal microcephaly and Guillain-Barré syndrome	284:377	In 2015-16, Zika virus (ZIKV) had an outbreak in South America associated with prenatal microcephaly and Guillain-Barré syndrome.					
36298752	2	61	contain	had	280:282	arg1	ZIKV					274:277	ZIKV	274:277	ZIKV	274:277	In 2015-16, Zika virus (ZIKV) had an outbreak in South America associated with prenatal microcephaly and Guillain-Barré syndrome.					
36298752	14	62	theme	genus	1971:1975	arg1	Bacteroides					1977:1987	the genus Bacteroides	1967:1987	the genus Bacteroides	1967:1987	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	14	62	theme	genus	1971:1975	arg1	related					2000:2006	related	2000:2006	related	2000:2006	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	4	63	theme	cocirculation	523:535	arg1	conditions					509:518	The conditions	505:518	The conditions of cocirculation of these three arboviruses	505:562	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	63	theme	cocirculation	523:535	arg1	lack					689:692	lack	689:692	lack of effective treatment or vaccines	689:727	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	63	theme	cocirculation	523:535	arg1	failure					565:571	failure	565:571	failure in vector control due to insecticide resistance	565:619	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	63	theme	cocirculation	523:535	arg1	limitations					622:632	limitations	622:632	limitations in dengue management during the COVID-19 pandemic	622:682	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	64	theme	midgut	779:784	arg1	composition					796:806	mosquito midgut bacterial composition	770:806	mosquito midgut bacterial composition	770:806	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	0	65	with	Association	0:10	arg1	Infection					70:78	Zika Infection	65:78	Zika Infection	65:78	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	0	65	with	Association	0:10	arg1	Resistance					96:105	Insecticide Resistance	84:105	Insecticide Resistance	84:105	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	13	66	theme	non-infective	1806:1818	arg1	form					1820:1823	an infective and non-infective form	1789:1823	an infective and non-infective form	1789:1823	In the face of natural infection with an arbovirus such as Zika, the diversity between an infective and non-infective form was significantly different.					
36298752	4	67	theme	insecticide	598:608	arg1	resistance					610:619	insecticide resistance	598:619	insecticide resistance	598:619	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	21	68	theme	resistance	3084:3093	arg1	condition					3071:3079	The condition	3067:3079	The condition of resistance to lambda-cyhalothrin	3067:3115	The condition of resistance to lambda-cyhalothrin could be inducing a phenome of dysbiosis in field Ae.					
36298752	19	69	theme	ZIKV	2987:2990	arg1	infection					2992:3000	ZIKV infection	2987:3000	ZIKV infection	2987:3000	CONCLUSIONS Microbiota composition may be modulated by ZIKV infection and insecticide resistance in Ae.					
36298752	21	70	from	phenome	3137:3143	arg1	Ae					3167:3168	field Ae	3161:3168	field Ae	3161:3168	The condition of resistance to lambda-cyhalothrin could be inducing a phenome of dysbiosis in field Ae.					
36298752	7	71	theme	DADA2	1116:1120	arg1	Pipeline					1122:1129	DADA2 Pipeline	1116:1129	DADA2 Pipeline	1116:1129	aegypti populations were analyzed using DADA2 Pipeline, and their functions were predicted with PICRUSt2 analysis.					
36298752	17	72	dep	presented	2508:2516	arg1	decreased					2532:2540	decreased	2532:2540	presented significantly decreased functions related to phenazine production, potentially associated with infection control, and control mechanism functions for host defense and quorum sensing	2508:2698	The Serratia genus presented significantly decreased functions related to phenazine production, potentially associated with infection control, and control mechanism functions for host defense and quorum sensing.					
36298752	16	73	theme	ZIKV-infected	2466:2478	arg1	females					2480:2486	ZIKV-infected females	2466:2486	ZIKV-infected females	2466:2486	On the other hand, Bello, the only mosquito population classified as susceptible to lambda-cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females.					
36298752	12	74	with	municipalities	1632:1645	arg1	supply					1668:1673	deficient water supply	1652:1673	deficient water supply	1652:1673	aegypti females from Colombian rural municipalities with deficient water supply, such as Honda with 63.6%.					
36298752	4	75	theme	vector	576:581	arg1	control					583:589	vector control	576:589	vector control	576:589	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	13	76	theme	infective	1792:1800	arg1	form					1820:1823	an infective and non-infective form	1789:1823	an infective and non-infective form	1789:1823	In the face of natural infection with an arbovirus such as Zika, the diversity between an infective and non-infective form was significantly different.					
36298752	11	77	theme	ZIKV	1567:1570	arg1	rates					1582:1586	high ZIKV infection rates	1562:1586	high ZIKV infection rates	1562:1586	RESULTS We found high ZIKV infection rates in Ae.					
36298752	2	78	theme	Zika	262:265	arg1	virus					267:271	Zika virus	262:271	Zika virus (ZIKV)	262:278	In 2015-16, Zika virus (ZIKV) had an outbreak in South America associated with prenatal microcephaly and Guillain-Barré syndrome.					
36298752	2	78	theme	Zika	262:265	arg1	ZIKV					274:277	ZIKV	274:277	ZIKV	274:277	In 2015-16, Zika virus (ZIKV) had an outbreak in South America associated with prenatal microcephaly and Guillain-Barré syndrome.					
36298752	5	79	from	level	1003:1007	arg1	risk					954:957	the risk	950:957	the risk of outbreaks of these diseases at the local level	950:1007	Its study is fundamental because it generates knowledge for surveillance of transmission and the risk of outbreaks of these diseases at the local level.					
36298752	5	79	from	level	1003:1007	arg1	surveillance					917:928	surveillance	917:928	surveillance of transmission and the risk of outbreaks of these diseases at the local level	917:1007	Its study is fundamental because it generates knowledge for surveillance of transmission and the risk of outbreaks of these diseases at the local level.					
36298752	5	79	from	level	1003:1007	arg1	transmission					933:944	transmission	933:944	transmission	933:944	Its study is fundamental because it generates knowledge for surveillance of transmission and the risk of outbreaks of these diseases at the local level.					
36298752	15	80	theme	human	2166:2170	arg1	infections					2183:2192	human intestinal infections	2166:2192	human intestinal infections	2166:2192	We hypothesize that it is a vehicle for virus entry, as it is in human intestinal infections.					
36298752	0	81	theme	Metabolic	41:49	arg1	Pathways					51:58	Their Metabolic Pathways	35:58	Their Metabolic Pathways	35:58	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	8	82	theme	natural	1239:1245	arg1	infection					1252:1260	natural ZIKV infection	1239:1260	natural ZIKV infection	1239:1260	These analyses were done under the condition of natural ZIKV infection and resistance to lambda-cyhalothrin, alone and in combination.					
36298752	3	83	with	interactions	475:486	arg1	vector					497:502	the vector	493:502	the vector	493:502	This mosquito's viral transmission is influenced by microbiota abundance and diversity and its interactions with the vector.					
36298752	18	84	theme	tryptophan	2825:2834	arg1	metabolism					2836:2845	tryptophan metabolism	2825:2845	tryptophan metabolism	2825:2845	Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis.					
36298752	22	85	theme	arboviruses	3209:3219	arg1	transmission					3193:3204	the transmission	3189:3204	the transmission of arboviruses	3189:3219	aegypti affecting the transmission of arboviruses.					
36298752	8	86	theme	infection	1252:1260	arg1	condition					1226:1234	the condition	1222:1234	the condition of natural ZIKV infection and resistance to lambda-cyhalothrin	1222:1297	These analyses were done under the condition of natural ZIKV infection and resistance to lambda-cyhalothrin, alone and in combination.					
36298752	19	87	theme	Microbiota	2944:2953	arg1	composition					2955:2965	Microbiota composition	2944:2965	Microbiota composition	2944:2965	CONCLUSIONS Microbiota composition may be modulated by ZIKV infection and insecticide resistance in Ae.					
36298752	3	88	with	microbiota	432:441	arg1	vector					497:502	the vector	493:502	the vector	493:502	This mosquito's viral transmission is influenced by microbiota abundance and diversity and its interactions with the vector.					
36298752	18	89	theme	related	2814:2820	arg1	functions					2769:2777	functions	2769:2777	functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis	2769:2929	Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis.					
36298752	1	90	theme	arboviruses	199:209	arg1	vector					181:186	the vector	177:186	the vector of several arboviruses such as dengue, Zika, and chikungunya	177:247	INTRODUCTION Aedes aegypti is the vector of several arboviruses such as dengue, Zika, and chikungunya.					
36298752	1	90	theme	arboviruses	199:209	arg1	INTRODUCTION					147:158	INTRODUCTION	147:158	INTRODUCTION Aedes aegypti	147:172	INTRODUCTION Aedes aegypti is the vector of several arboviruses such as dengue, Zika, and chikungunya.					
36298752	12	91	theme	water	1662:1666	arg1	supply					1668:1673	deficient water supply	1652:1673	deficient water supply	1652:1673	aegypti females from Colombian rural municipalities with deficient water supply, such as Honda with 63.6%.					
36298752	18	92	theme	ABC	2848:2850	arg1	transporters					2852:2863	ABC transporters	2848:2863	ABC transporters with a two-component system	2848:2891	Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis.					
36298752	8	93	theme	resistance	1266:1275	arg1	condition					1226:1234	the condition	1222:1234	the condition of natural ZIKV infection and resistance to lambda-cyhalothrin	1222:1297	These analyses were done under the condition of natural ZIKV infection and resistance to lambda-cyhalothrin, alone and in combination.					
36298752	18	94	theme	degradation	2786:2796	arg1	functions					2769:2777	functions	2769:2777	functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis	2769:2929	Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis.					
36298752	14	95	theme	lambda-cyhalothrin	1914:1931	arg1	resistance					1933:1942	lambda-cyhalothrin resistance	1914:1942	lambda-cyhalothrin resistance	1914:1942	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	16	96	from	functions	2357:2365	arg1	intestine					2374:2382	the intestine	2370:2382	the intestine	2370:2382	On the other hand, Bello, the only mosquito population classified as susceptible to lambda-cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females.					
36298752	12	97	theme	rural	1626:1630	arg1	Honda					1684:1688	Honda	1684:1688	Honda	1684:1688	aegypti females from Colombian rural municipalities with deficient water supply, such as Honda with 63.6%.					
36298752	12	97	theme	rural	1626:1630	arg1	municipalities					1632:1645	Colombian rural municipalities	1616:1645	Colombian rural municipalities	1616:1645	aegypti females from Colombian rural municipalities with deficient water supply, such as Honda with 63.6%.					
36298752	21	98	theme	field	3161:3165	arg1	Ae					3167:3168	field Ae	3161:3168	field Ae	3161:3168	The condition of resistance to lambda-cyhalothrin could be inducing a phenome of dysbiosis in field Ae.					
36298752	6	99	theme	bacterial	1025:1033	arg1	compositions					1035:1046	Midgut bacterial compositions	1018:1046	Midgut bacterial compositions of females of Colombian Ae	1018:1073	METHODS Midgut bacterial compositions of females of Colombian Ae.					
36298752	19	100	dep	CONCLUSIONS	2932:2942	arg1	modulated					2974:2982	modulated	2974:2982	may be modulated by ZIKV infection and insecticide resistance in Ae	2967:3033	CONCLUSIONS Microbiota composition may be modulated by ZIKV infection and insecticide resistance in Ae.					
36298752	0	101	from	Resistance	96:105	arg1	Populations					134:144	Colombian Aedes aegypti Populations	110:144	Colombian Aedes aegypti Populations	110:144	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	14	102	theme	antimicrobial	2039:2051	arg1	resistance					2053:2062	antimicrobial resistance	2039:2062	antimicrobial resistance	2039:2062	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	17	103	theme	mechanism	2644:2652	arg1	functions					2654:2662	control mechanism functions	2636:2662	control mechanism functions	2636:2662	The Serratia genus presented significantly decreased functions related to phenazine production, potentially associated with infection control, and control mechanism functions for host defense and quorum sensing.					
36298752	4	104	theme	treatment	707:715	arg1	conditions					509:518	The conditions	505:518	The conditions of cocirculation of these three arboviruses	505:562	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	104	theme	treatment	707:715	arg1	lack					689:692	lack	689:692	lack of effective treatment or vaccines	689:727	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	104	theme	treatment	707:715	arg1	failure					565:571	failure	565:571	failure in vector control due to insecticide resistance	565:619	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	104	theme	treatment	707:715	arg1	limitations					622:632	limitations	622:632	limitations in dengue management during the COVID-19 pandemic	622:682	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	0	105	theme	Zika	65:68	arg1	Infection					70:78	Zika Infection	65:78	Zika Infection	65:78	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	12	106	theme	aegypti	1595:1601	arg1	females					1603:1609	aegypti females	1595:1609	aegypti females from Colombian rural municipalities with deficient water supply, such as Honda with 63.6%.	1595:1700	aegypti females from Colombian rural municipalities with deficient water supply, such as Honda with 63.6%.					
36298752	0	107	theme	Aedes	120:124	arg1	Populations					134:144	Colombian Aedes aegypti Populations	110:144	Colombian Aedes aegypti Populations	110:144	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	13	108	theme	natural	1717:1723	arg1	infection					1725:1733	natural infection	1717:1733	natural infection with an arbovirus such as Zika	1717:1764	In the face of natural infection with an arbovirus such as Zika, the diversity between an infective and non-infective form was significantly different.					
36298752	16	109	theme	related	2328:2334	arg1	bacteria					2319:2326	bacteria	2319:2326	bacteria	2319:2326	On the other hand, Bello, the only mosquito population classified as susceptible to lambda-cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females.					
36298752	18	110	theme	efflux	2894:2899	arg1	pumps					2901:2905	efflux pumps	2894:2905	efflux pumps	2894:2905	Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis.					
36298752	4	111	from	changes	759:765	arg1	composition					796:806	mosquito midgut bacterial composition	770:806	mosquito midgut bacterial composition	770:806	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	5	112	theme	outbreaks	962:970	arg1	risk					954:957	the risk	950:957	the risk of outbreaks of these diseases at the local level	950:1007	Its study is fundamental because it generates knowledge for surveillance of transmission and the risk of outbreaks of these diseases at the local level.					
36298752	5	112	theme	outbreaks	962:970	arg1	transmission					933:944	transmission	933:944	transmission	933:944	Its study is fundamental because it generates knowledge for surveillance of transmission and the risk of outbreaks of these diseases at the local level.					
36298752	19	113	theme	insecticide	3006:3016	arg1	resistance					3018:3027	insecticide resistance	3006:3027	insecticide resistance	3006:3027	CONCLUSIONS Microbiota composition may be modulated by ZIKV infection and insecticide resistance in Ae.					
36298752	0	114	theme	Colombian	110:118	arg1	Populations					134:144	Colombian Aedes aegypti Populations	110:144	Colombian Aedes aegypti Populations	110:144	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	9	115	theme	One-step	1326:1333	arg1	RT-PCR					1335:1340	One-step RT-PCR	1326:1340	One-step RT-PCR	1326:1340	One-step RT-PCR determined the percentage of ZIKV-infected females.					
36298752	14	116	theme	pathogenicity	2024:2036	arg1	functions					2011:2019	functions	2011:2019	functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides	2011:2098	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	10	117	theme	mutation	1508:1515	arg1	presence					1485:1492	the presence	1481:1492	the presence of the V1016I mutation in the sodium channel gene	1481:1542	We also measured the susceptibility to the pyrethroid lambda-cyhalothrin and evaluated the presence of the V1016I mutation in the sodium channel gene.					
36298752	16	118	theme	mosquito	2230:2237	arg1	population					2239:2248	the only mosquito population	2221:2248	the only mosquito population classified as susceptible to lambda-cyhalothrin	2221:2296	On the other hand, Bello, the only mosquito population classified as susceptible to lambda-cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females.					
36298752	16	118	theme	mosquito	2230:2237	arg1	Bello					2214:2218	Bello	2214:2218	Bello	2214:2218	On the other hand, Bello, the only mosquito population classified as susceptible to lambda-cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females.					
36298752	12	119	theme	deficient	1652:1660	arg1	supply					1668:1673	deficient water supply	1652:1673	deficient water supply	1652:1673	aegypti females from Colombian rural municipalities with deficient water supply, such as Honda with 63.6%.					
36298752	6	120	theme	Ae	1072:1073	arg1	females					1051:1057	females	1051:1057	females of Colombian Ae	1051:1073	METHODS Midgut bacterial compositions of females of Colombian Ae.					
36298752	4	121	theme	vaccines	720:727	arg1	conditions					509:518	The conditions	505:518	The conditions of cocirculation of these three arboviruses	505:562	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	121	theme	vaccines	720:727	arg1	lack					689:692	lack	689:692	lack of effective treatment or vaccines	689:727	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	121	theme	vaccines	720:727	arg1	failure					565:571	failure	565:571	failure in vector control due to insecticide resistance	565:619	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	121	theme	vaccines	720:727	arg1	limitations					622:632	limitations	622:632	limitations in dengue management during the COVID-19 pandemic	622:682	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	0	122	theme	Bacteria	22:29	arg1	Association					0:10	Association	0:10	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.	0:145	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	13	123	from	face	1709:1712	arg1	different					1843:1851	different	1843:1851	different	1843:1851	In the face of natural infection with an arbovirus such as Zika, the diversity between an infective and non-infective form was significantly different.					
36298752	13	123	from	face	1709:1712	arg1	diversity					1771:1779	the diversity	1767:1779	the diversity between an infective and non-infective form	1767:1823	In the face of natural infection with an arbovirus such as Zika, the diversity between an infective and non-infective form was significantly different.					
36298752	5	124	from	surveillance	917:928	arg1	level					1003:1007	the local level	993:1007	the local level	993:1007	Its study is fundamental because it generates knowledge for surveillance of transmission and the risk of outbreaks of these diseases at the local level.					
36298752	5	125	theme	risk	954:957	arg1	surveillance					917:928	surveillance	917:928	surveillance of transmission and the risk of outbreaks of these diseases at the local level	917:1007	Its study is fundamental because it generates knowledge for surveillance of transmission and the risk of outbreaks of these diseases at the local level.					
36298752	18	126	with	transporters	2852:2863	arg1	system					2886:2891	a two-component system	2870:2891	a two-component system	2870:2891	Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis.					
36298752	10	127	theme	channel	1531:1537	arg1	gene					1539:1542	the sodium channel gene	1520:1542	the sodium channel gene	1520:1542	We also measured the susceptibility to the pyrethroid lambda-cyhalothrin and evaluated the presence of the V1016I mutation in the sodium channel gene.					
36298752	1	128	dep	Aedes	160:164	arg1	aegypti					166:172	aegypti	166:172	aegypti	166:172	INTRODUCTION Aedes aegypti is the vector of several arboviruses such as dengue, Zika, and chikungunya.					
36298752	9	129	theme	ZIKV-infected	1371:1383	arg1	females					1385:1391	ZIKV-infected females	1371:1391	ZIKV-infected females	1371:1391	One-step RT-PCR determined the percentage of ZIKV-infected females.					
36298752	3	130	theme	viral	396:400	arg1	transmission					402:413	This mosquito's viral transmission	380:413	This mosquito's viral transmission	380:413	This mosquito's viral transmission is influenced by microbiota abundance and diversity and its interactions with the vector.					
36298752	0	131	theme	Pathways	51:58	arg1	Association					0:10	Association	0:10	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.	0:145	Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations.					
36298752	14	132	with	infection	1890:1898	arg1	ZIKV					1905:1908	ZIKV	1905:1908	ZIKV	1905:1908	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	14	132	with	infection	1890:1898	arg1	resistance					1933:1942	lambda-cyhalothrin resistance	1914:1942	lambda-cyhalothrin resistance	1914:1942	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	13	133	from	different	1843:1851	arg1	face					1709:1712	the face	1705:1712	the face of natural infection with an arbovirus such as Zika	1705:1764	In the face of natural infection with an arbovirus such as Zika, the diversity between an infective and non-infective form was significantly different.					
36298752	16	134	theme	Lachnospiraceae	2402:2416	arg1	family					2418:2423	the Lachnospiraceae family	2398:2423	the Lachnospiraceae family	2398:2423	On the other hand, Bello, the only mosquito population classified as susceptible to lambda-cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females.					
36298752	3	135	dep	microbiota	432:441	arg1	abundance					443:451	abundance	443:451	abundance	443:451	This mosquito's viral transmission is influenced by microbiota abundance and diversity and its interactions with the vector.					
36298752	17	136	theme	Serratia	2493:2500	arg1	genus					2502:2506	The Serratia genus	2489:2506	The Serratia genus	2489:2506	The Serratia genus presented significantly decreased functions related to phenazine production, potentially associated with infection control, and control mechanism functions for host defense and quorum sensing.					
36298752	14	137	theme	resistance	2053:2062	arg1	functions					2011:2019	functions	2011:2019	functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides	2011:2098	Bacteria associated with a state of infection with ZIKV and lambda-cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides.					
36298752	4	138	theme	mosquito	770:777	arg1	composition					796:806	mosquito midgut bacterial composition	770:806	mosquito midgut bacterial composition	770:806	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	5	139	theme	local	997:1001	arg1	level					1003:1007	the local level	993:1007	the local level	993:1007	Its study is fundamental because it generates knowledge for surveillance of transmission and the risk of outbreaks of these diseases at the local level.					
36298752	4	140	theme	bacterial	786:794	arg1	composition					796:806	mosquito midgut bacterial composition	770:806	mosquito midgut bacterial composition	770:806	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	18	141	theme	two-component	2872:2884	arg1	system					2886:2891	a two-component system	2870:2891	a two-component system	2870:2891	Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis.					
36298752	2	142	theme	prenatal	329:336	arg1	microcephaly					338:349	prenatal microcephaly	329:349	prenatal microcephaly	329:349	In 2015-16, Zika virus (ZIKV) had an outbreak in South America associated with prenatal microcephaly and Guillain-Barré syndrome.					
36298752	4	143	from	lack	689:692	arg1	control					583:589	vector control	576:589	vector control	576:589	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36298752	4	143	from	lack	689:692	arg1	management					644:653	dengue management	637:653	dengue management during the COVID-19 pandemic	637:682	The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection.					
36574802	8	0	with	times	1678:1682	arg1	loss					1692:1695	no loss	1689:1695	no loss of the initial shape, size, and morphology	1689:1738	Moreover, Pd-CS-CAC was reused for 5 consecutive times with no loss of the initial shape, size, and morphology, confirming that it was a sustainable and robust nanocatalyst.					
36574802	6	1	theme	easy	1321:1324	arg1	separation					1326:1335	easy separation	1321:1335	easy separation	1321:1335	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	1	2	theme	biodegradable	282:294	arg1	support					342:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support	263:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	263:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	1	3	from	nanocatalyst	247:258	arg1	support					342:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support	263:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	263:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	1	4	from	support	342:348	arg1	fabrication					194:204	the fabrication	190:204	the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	190:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	4	5	theme	blue	977:980	arg1	MB					983:984	MB	983:984	MB	983:984	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	5	theme	blue	977:980	arg1	orange					954:959	methyl orange	947:959	methyl orange (MO)	947:964	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	5	theme	blue	977:980	arg1	reduction/decolorization					755:778	the reduction/decolorization	751:778	the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes	751:927	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	6	6	theme	due	1387:1389	arg1	catalyst					1378:1385	the catalyst	1374:1385	the catalyst due to its heterogeneity	1374:1410	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	7	7	theme	reduction/decolorization	1500:1523	arg1	reactions					1525:1533	reduction/decolorization reactions	1500:1533	reduction/decolorization reactions	1500:1533	The results of the tests showed that reduction/decolorization reactions were successfully carried out within 10-140 s due to the good catalytic ability of Pd-CS-CAC.					
36574802	2	8	theme	EDS	578:580	arg1	techniques					582:591	FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques	545:591	FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques	545:591	The physicochemical properties of the microporous Pd-CS-CAC nanocatalyst developed were successfully determined by FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques.					
36574802	4	9	theme	rhodamine	1008:1016	arg1	RhB					1021:1023	RhB	1021:1023	RhB	1021:1023	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	9	theme	rhodamine	1008:1016	arg1	B					1018:1018	rhodamine B	1008:1018	rhodamine B (RhB))	1008:1025	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	1	10	theme	heterogeneous	223:235	arg1	nanocatalyst					247:258	a microporous heterogeneous palladium nanocatalyst	209:258	a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	209:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	4	11	theme	methyl	930:935	arg1	MR					942:943	methyl red (MR)	930:944	methyl red (MR)	930:944	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	3	12	theme	average	622:628	arg1	size					639:642	the average particle size	618:642	the average particle size of the synthesized Pd NPs	618:668	TEM studies showed that the average particle size of the synthesized Pd NPs was about 30 nm.					
36574802	1	13	theme	nanocatalyst	247:258	arg1	fabrication					194:204	the fabrication	190:204	the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	190:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	7	14	theme	Pd-CS-CAC	1618:1626	arg1	ability					1607:1613	the good catalytic ability	1588:1613	the good catalytic ability of Pd-CS-CAC	1588:1626	The results of the tests showed that reduction/decolorization reactions were successfully carried out within 10-140 s due to the good catalytic ability of Pd-CS-CAC.					
36574802	5	15	from	intervals	1223:1231	arg1	spectroscopy					1175:1186	UV-Vis absorption spectroscopy	1157:1186	UV-Vis absorption spectroscopy of the supernatant at regular time intervals	1157:1231	The catalytic activities were studied by UV-Vis absorption spectroscopy of the supernatant at regular time intervals.					
36574802	4	16	theme	NaBH4	1067:1071	arg1	presence					1055:1062	the presence	1051:1062	the presence of NaBH4	1051:1071	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	5	17	theme	UV-Vis	1157:1162	arg1	spectroscopy					1175:1186	UV-Vis absorption spectroscopy	1157:1186	UV-Vis absorption spectroscopy of the supernatant at regular time intervals	1157:1231	The catalytic activities were studied by UV-Vis absorption spectroscopy of the supernatant at regular time intervals.					
36574802	4	18	theme	methyl	947:952	arg1	MO					962:963	MO	962:963	MO	962:963	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	18	theme	methyl	947:952	arg1	orange					954:959	methyl orange	947:959	methyl orange (MO)	947:964	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	18	theme	methyl	947:952	arg1	reduction/decolorization					755:778	the reduction/decolorization	751:778	the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes	751:927	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	19	theme	aqueous	1033:1039	arg1	medium					1041:1046	an aqueous medium	1030:1046	an aqueous medium	1030:1046	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	6	20	theme	reaction	1265:1272	arg1	times					1253:1257	The short reaction times	1234:1257	The short reaction times	1234:1257	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	20	theme	reaction	1265:1272	arg1	conditions					1274:1283	mild reaction conditions	1260:1283	mild reaction conditions	1260:1283	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	21	theme	100	1303:1305	arg1	%					1306:1306	%	1306:1306	%	1306:1306	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	8	22	theme	consecutive	1666:1676	arg1	times					1678:1682	5 consecutive times	1664:1682	5 consecutive times with no loss of the initial shape, size, and morphology	1664:1738	Moreover, Pd-CS-CAC was reused for 5 consecutive times with no loss of the initial shape, size, and morphology, confirming that it was a sustainable and robust nanocatalyst.					
36574802	1	23	theme	versatile	168:176	arg1	process					178:184	a versatile process	166:184	a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	166:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	7	24	theme	good	1592:1595	arg1	ability					1607:1613	the good catalytic ability	1588:1613	the good catalytic ability of Pd-CS-CAC	1588:1626	The results of the tests showed that reduction/decolorization reactions were successfully carried out within 10-140 s due to the good catalytic ability of Pd-CS-CAC.					
36574802	4	25	theme	microporous	712:722	arg1	Pd-CS-CAC					724:732	microporous Pd-CS-CAC	712:732	microporous Pd-CS-CAC	712:732	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	26	dep	nitroarenes	791:801	arg1	4-nitrophenol					850:862	4-nitrophenol	850:862	4-nitrophenol (4-NP)	850:869	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	26	dep	nitroarenes	791:801	arg1	4-nitroaniline					827:840	4-nitroaniline	827:840	4-nitroaniline (4-NA)	827:847	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	26	dep	nitroarenes	791:801	arg1	nitroarenes					791:801	various nitroarenes	783:801	various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD))	783:910	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	26	dep	nitroarenes	791:801	arg1	4-NP					865:868	4-NP	865:868	4-NP	865:868	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	26	dep	nitroarenes	791:801	arg1	4-NPD					904:908	4-NPD	904:908	4-NPD	904:908	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	26	dep	nitroarenes	791:801	arg1	2-nitroaniline					804:817	2-nitroaniline	804:817	2-nitroaniline (2-NA)	804:824	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	26	dep	nitroarenes	791:801	arg1	4-NA					843:846	4-NA	843:846	4-NA	843:846	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	26	dep	nitroarenes	791:801	arg1	2-NA					820:823	2-NA	820:823	2-NA	820:823	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	26	dep	nitroarenes	791:801	arg1	4-nitro-o-phenylenediamine					876:901	4-nitro-o-phenylenediamine	876:901	4-nitro-o-phenylenediamine	876:901	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	27	theme	room	1098:1101	arg1	temperature					1103:1113	room temperature	1098:1113	room temperature	1098:1113	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	6	28	theme	reaction	1244:1251	arg1	efficiency					1291:1300	high efficiency	1286:1300	high efficiency (100% conversion)	1286:1318	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	28	theme	reaction	1244:1251	arg1	reusability					1416:1426	reusability	1416:1426	reusability	1416:1426	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	28	theme	reaction	1244:1251	arg1	times					1253:1257	The short reaction times	1234:1257	The short reaction times	1234:1257	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	28	theme	reaction	1244:1251	arg1	advantages					1436:1445	the advantages	1432:1445	the advantages of this method	1432:1460	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	28	theme	reaction	1244:1251	arg1	conditions					1274:1283	mild reaction conditions	1260:1283	mild reaction conditions	1260:1283	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	1	29	theme	waste-derived	392:404	arg1	CAC					424:426	CAC	424:426	CAC	424:426	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	1	29	theme	waste-derived	392:404	arg1	carbon					416:421	cigarette waste-derived activated carbon	382:421	cigarette waste-derived activated carbon (CAC)	382:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	5	30	theme	time	1218:1221	arg1	intervals					1223:1231	regular time intervals	1210:1231	regular time intervals	1210:1231	The catalytic activities were studied by UV-Vis absorption spectroscopy of the supernatant at regular time intervals.					
36574802	0	31	theme	environmental	119:131	arg1	contaminants					133:144	environmental contaminants	119:144	environmental contaminants	119:144	Nano-sized and microporous palladium catalyst supported on modified chitosan/cigarette butt composite for treatment of environmental contaminants.					
36574802	0	32	theme	Nano-sized	0:9	arg1	catalyst					37:44	Nano-sized and microporous palladium catalyst	0:44	Nano-sized and microporous palladium catalyst	0:44	Nano-sized and microporous palladium catalyst supported on modified chitosan/cigarette butt composite for treatment of environmental contaminants.					
36574802	8	33	theme	shape	1712:1716	arg1	loss					1692:1695	no loss	1689:1695	no loss of the initial shape, size, and morphology	1689:1738	Moreover, Pd-CS-CAC was reused for 5 consecutive times with no loss of the initial shape, size, and morphology, confirming that it was a sustainable and robust nanocatalyst.					
36574802	2	34	theme	Pd-CS-CAC	480:488	arg1	nanocatalyst					490:501	the microporous Pd-CS-CAC nanocatalyst	464:501	the microporous Pd-CS-CAC nanocatalyst developed	464:511	The physicochemical properties of the microporous Pd-CS-CAC nanocatalyst developed were successfully determined by FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques.					
36574802	4	35	theme	various	783:789	arg1	4-nitro-o-phenylenediamine					876:901	4-nitro-o-phenylenediamine	876:901	4-nitro-o-phenylenediamine	876:901	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	35	theme	various	783:789	arg1	4-nitrophenol					850:862	4-nitrophenol	850:862	4-nitrophenol (4-NP)	850:869	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	35	theme	various	783:789	arg1	4-nitroaniline					827:840	4-nitroaniline	827:840	4-nitroaniline (4-NA)	827:847	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	35	theme	various	783:789	arg1	2-nitroaniline					804:817	2-nitroaniline	804:817	2-nitroaniline (2-NA)	804:824	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	35	theme	various	783:789	arg1	nitroarenes					791:801	various nitroarenes	783:801	various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD))	783:910	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	0	36	theme	microporous	15:25	arg1	catalyst					37:44	Nano-sized and microporous palladium catalyst	0:44	Nano-sized and microporous palladium catalyst	0:44	Nano-sized and microporous palladium catalyst supported on modified chitosan/cigarette butt composite for treatment of environmental contaminants.					
36574802	1	37	theme	catalyst	333:340	arg1	support					342:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support	263:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	263:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	0	38	theme	chitosan/cigarette	68:85	arg1	composite					92:100	modified chitosan/cigarette butt composite	59:100	modified chitosan/cigarette butt composite for treatment of environmental contaminants	59:144	Nano-sized and microporous palladium catalyst supported on modified chitosan/cigarette butt composite for treatment of environmental contaminants.					
36574802	3	39	theme	Pd	663:664	arg1	NPs					666:668	the synthesized Pd NPs	647:668	the synthesized Pd NPs	647:668	TEM studies showed that the average particle size of the synthesized Pd NPs was about 30 nm.					
36574802	8	40	theme	morphology	1729:1738	arg1	loss					1692:1695	no loss	1689:1695	no loss of the initial shape, size, and morphology	1689:1738	Moreover, Pd-CS-CAC was reused for 5 consecutive times with no loss of the initial shape, size, and morphology, confirming that it was a sustainable and robust nanocatalyst.					
36574802	6	41	theme	chemical	1352:1359	arg1	stability					1361:1369	excellent chemical stability	1342:1369	excellent chemical stability	1342:1369	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	42	theme	%	1306:1306	arg1	efficiency					1291:1300	high efficiency	1286:1300	high efficiency (100% conversion)	1286:1318	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	42	theme	%	1306:1306	arg1	conversion					1308:1317	100% conversion	1303:1317	100% conversion	1303:1317	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	1	43	theme	novel	265:269	arg1	support					342:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support	263:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	263:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	6	44	theme	high	1286:1289	arg1	efficiency					1291:1300	high efficiency	1286:1300	high efficiency (100% conversion)	1286:1318	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	44	theme	high	1286:1289	arg1	times					1253:1257	The short reaction times	1234:1257	The short reaction times	1234:1257	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	44	theme	high	1286:1289	arg1	conversion					1308:1317	100% conversion	1303:1317	100% conversion	1303:1317	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	8	45	theme	size	1719:1722	arg1	loss					1692:1695	no loss	1689:1695	no loss of the initial shape, size, and morphology	1689:1738	Moreover, Pd-CS-CAC was reused for 5 consecutive times with no loss of the initial shape, size, and morphology, confirming that it was a sustainable and robust nanocatalyst.					
36574802	3	46	theme	TEM	594:596	arg1	studies					598:604	TEM studies	594:604	TEM studies	594:604	TEM studies showed that the average particle size of the synthesized Pd NPs was about 30 nm.					
36574802	6	47	theme	catalyst	1378:1385	arg1	efficiency					1291:1300	high efficiency	1286:1300	high efficiency (100% conversion)	1286:1318	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	47	theme	catalyst	1378:1385	arg1	separation					1326:1335	easy separation	1321:1335	easy separation	1321:1335	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	47	theme	catalyst	1378:1385	arg1	conditions					1274:1283	mild reaction conditions	1260:1283	mild reaction conditions	1260:1283	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	47	theme	catalyst	1378:1385	arg1	reusability					1416:1426	reusability	1416:1426	reusability	1416:1426	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	47	theme	catalyst	1378:1385	arg1	conversion					1308:1317	100% conversion	1303:1317	100% conversion	1303:1317	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	47	theme	catalyst	1378:1385	arg1	times					1253:1257	The short reaction times	1234:1257	The short reaction times	1234:1257	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	47	theme	catalyst	1378:1385	arg1	stability					1361:1369	excellent chemical stability	1342:1369	excellent chemical stability	1342:1369	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	1	48	theme	microporous	211:221	arg1	nanocatalyst					247:258	a microporous heterogeneous palladium nanocatalyst	209:258	a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	209:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	7	49	theme	tests	1482:1486	arg1	results					1467:1473	The results	1463:1473	The results of the tests	1463:1486	The results of the tests showed that reduction/decolorization reactions were successfully carried out within 10-140 s due to the good catalytic ability of Pd-CS-CAC.					
36574802	4	50	theme	organic	916:922	arg1	dyes					924:927	organic dyes	916:927	organic dyes	916:927	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	8	51	theme	sustainable	1766:1776	arg1	it					1757:1758	it	1757:1758	it	1757:1758	Moreover, Pd-CS-CAC was reused for 5 consecutive times with no loss of the initial shape, size, and morphology, confirming that it was a sustainable and robust nanocatalyst.					
36574802	8	51	theme	sustainable	1766:1776	arg1	nanocatalyst					1789:1800	a sustainable and robust nanocatalyst	1764:1800	a sustainable and robust nanocatalyst	1764:1800	Moreover, Pd-CS-CAC was reused for 5 consecutive times with no loss of the initial shape, size, and morphology, confirming that it was a sustainable and robust nanocatalyst.					
36574802	1	52	theme	palladium	237:245	arg1	nanocatalyst					247:258	a microporous heterogeneous palladium nanocatalyst	209:258	a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	209:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	2	53	theme	BET	569:571	arg1	techniques					582:591	FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques	545:591	FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques	545:591	The physicochemical properties of the microporous Pd-CS-CAC nanocatalyst developed were successfully determined by FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques.					
36574802	8	54	theme	robust	1782:1787	arg1	it					1757:1758	it	1757:1758	it	1757:1758	Moreover, Pd-CS-CAC was reused for 5 consecutive times with no loss of the initial shape, size, and morphology, confirming that it was a sustainable and robust nanocatalyst.					
36574802	8	54	theme	robust	1782:1787	arg1	nanocatalyst					1789:1800	a sustainable and robust nanocatalyst	1764:1800	a sustainable and robust nanocatalyst	1764:1800	Moreover, Pd-CS-CAC was reused for 5 consecutive times with no loss of the initial shape, size, and morphology, confirming that it was a sustainable and robust nanocatalyst.					
36574802	6	55	theme	method	1455:1460	arg1	advantages					1436:1445	the advantages	1432:1445	the advantages of this method	1432:1460	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	55	theme	method	1455:1460	arg1	times					1253:1257	The short reaction times	1234:1257	The short reaction times	1234:1257	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	4	56	theme	dyes	924:927	arg1	MB					983:984	MB	983:984	MB	983:984	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	56	theme	dyes	924:927	arg1	RhB					1021:1023	RhB	1021:1023	RhB	1021:1023	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	56	theme	dyes	924:927	arg1	orange					954:959	methyl orange	947:959	methyl orange (MO)	947:964	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	56	theme	dyes	924:927	arg1	reduction/decolorization					755:778	the reduction/decolorization	751:778	the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes	751:927	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	56	theme	dyes	924:927	arg1	B					1018:1018	rhodamine B	1008:1018	rhodamine B (RhB))	1008:1025	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	57	theme	Pd-CS-CAC	724:732	arg1	agent					1089:1093	the reducing agent	1076:1093	the reducing agent at room temperature	1076:1113	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	57	theme	Pd-CS-CAC	724:732	arg1	prowess					701:707	The catalytic prowess	687:707	The catalytic prowess of microporous Pd-CS-CAC	687:732	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	58	dep	reduction/decolorization	755:778	arg1	MR					942:943	methyl red (MR)	930:944	methyl red (MR)	930:944	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	5	59	theme	absorption	1164:1173	arg1	spectroscopy					1175:1186	UV-Vis absorption spectroscopy	1157:1186	UV-Vis absorption spectroscopy of the supernatant at regular time intervals	1157:1231	The catalytic activities were studied by UV-Vis absorption spectroscopy of the supernatant at regular time intervals.					
36574802	0	60	theme	butt	87:90	arg1	composite					92:100	modified chitosan/cigarette butt composite	59:100	modified chitosan/cigarette butt composite for treatment of environmental contaminants	59:144	Nano-sized and microporous palladium catalyst supported on modified chitosan/cigarette butt composite for treatment of environmental contaminants.					
36574802	3	61	theme	particle	630:637	arg1	size					639:642	the average particle size	618:642	the average particle size of the synthesized Pd NPs	618:668	TEM studies showed that the average particle size of the synthesized Pd NPs was about 30 nm.					
36574802	2	62	theme	XRD	551:553	arg1	techniques					582:591	FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques	545:591	FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques	545:591	The physicochemical properties of the microporous Pd-CS-CAC nanocatalyst developed were successfully determined by FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques.					
36574802	4	63	theme	catalytic	691:699	arg1	agent					1089:1093	the reducing agent	1076:1093	the reducing agent at room temperature	1076:1113	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	63	theme	catalytic	691:699	arg1	prowess					701:707	The catalytic prowess	687:707	The catalytic prowess of microporous Pd-CS-CAC	687:732	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	5	64	theme	catalytic	1120:1128	arg1	activities					1130:1139	The catalytic activities	1116:1139	The catalytic activities	1116:1139	The catalytic activities were studied by UV-Vis absorption spectroscopy of the supernatant at regular time intervals.					
36574802	4	65	theme	red	937:939	arg1	MR					942:943	methyl red (MR)	930:944	methyl red (MR)	930:944	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	6	66	theme	mild	1260:1263	arg1	times					1253:1257	The short reaction times	1234:1257	The short reaction times	1234:1257	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	66	theme	mild	1260:1263	arg1	conditions					1274:1283	mild reaction conditions	1260:1283	mild reaction conditions	1260:1283	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	1	67	from	fabrication	194:204	arg1	support					342:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support	263:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	263:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	7	68	theme	catalytic	1597:1605	arg1	ability					1607:1613	the good catalytic ability	1588:1613	the good catalytic ability of Pd-CS-CAC	1588:1626	The results of the tests showed that reduction/decolorization reactions were successfully carried out within 10-140 s due to the good catalytic ability of Pd-CS-CAC.					
36574802	2	69	theme	FTIR	545:548	arg1	techniques					582:591	FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques	545:591	FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques	545:591	The physicochemical properties of the microporous Pd-CS-CAC nanocatalyst developed were successfully determined by FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques.					
36574802	1	70	theme	cigarette	382:390	arg1	CAC					424:426	CAC	424:426	CAC	424:426	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	1	70	theme	cigarette	382:390	arg1	carbon					416:421	cigarette waste-derived activated carbon	382:421	cigarette waste-derived activated carbon (CAC)	382:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	2	71	theme	TEM	564:566	arg1	techniques					582:591	FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques	545:591	FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques	545:591	The physicochemical properties of the microporous Pd-CS-CAC nanocatalyst developed were successfully determined by FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques.					
36574802	4	72	theme	nitroarenes	791:801	arg1	MB					983:984	MB	983:984	MB	983:984	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	72	theme	nitroarenes	791:801	arg1	RhB					1021:1023	RhB	1021:1023	RhB	1021:1023	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	72	theme	nitroarenes	791:801	arg1	orange					954:959	methyl orange	947:959	methyl orange (MO)	947:964	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	72	theme	nitroarenes	791:801	arg1	reduction/decolorization					755:778	the reduction/decolorization	751:778	the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes	751:927	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	72	theme	nitroarenes	791:801	arg1	B					1018:1018	rhodamine B	1008:1018	rhodamine B (RhB))	1008:1025	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	5	73	theme	regular	1210:1216	arg1	intervals					1223:1231	regular time intervals	1210:1231	regular time intervals	1210:1231	The catalytic activities were studied by UV-Vis absorption spectroscopy of the supernatant at regular time intervals.					
36574802	0	74	theme	contaminants	133:144	arg1	treatment					106:114	treatment	106:114	treatment of environmental contaminants	106:144	Nano-sized and microporous palladium catalyst supported on modified chitosan/cigarette butt composite for treatment of environmental contaminants.					
36574802	1	75	theme	activated	406:414	arg1	CAC					424:426	CAC	424:426	CAC	424:426	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	1	75	theme	activated	406:414	arg1	carbon					416:421	cigarette waste-derived activated carbon	382:421	cigarette waste-derived activated carbon (CAC)	382:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	4	76	from	temperature	1103:1113	arg1	agent					1089:1093	the reducing agent	1076:1093	the reducing agent at room temperature	1076:1113	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	76	from	temperature	1103:1113	arg1	prowess					701:707	The catalytic prowess	687:707	The catalytic prowess of microporous Pd-CS-CAC	687:732	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	2	77	theme	FE-SEM	556:561	arg1	techniques					582:591	FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques	545:591	FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques	545:591	The physicochemical properties of the microporous Pd-CS-CAC nanocatalyst developed were successfully determined by FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques.					
36574802	4	78	theme	reducing	1080:1087	arg1	agent					1089:1093	the reducing agent	1076:1093	the reducing agent at room temperature	1076:1113	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	4	78	theme	reducing	1080:1087	arg1	prowess					701:707	The catalytic prowess	687:707	The catalytic prowess of microporous Pd-CS-CAC	687:732	The catalytic prowess of microporous Pd-CS-CAC was evaluated in the reduction/decolorization of various nitroarenes (2-nitroaniline (2-NA), 4-nitroaniline (4-NA), 4-nitrophenol (4-NP), and 4-nitro-o-phenylenediamine (4-NPD)) and organic dyes (methyl red (MR), methyl orange (MO), methylene blue (MB), congo red (CR), and rhodamine B (RhB)) in an aqueous medium in the presence of NaBH4 as the reducing agent at room temperature.					
36574802	1	79	link	waste-derived	392:404	arg1	CAC					424:426	CAC	424:426	CAC	424:426	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	1	79	link	waste-derived	392:404	arg1	carbon					416:421	cigarette waste-derived activated carbon	382:421	cigarette waste-derived activated carbon (CAC)	382:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	6	80	theme	short	1238:1242	arg1	efficiency					1291:1300	high efficiency	1286:1300	high efficiency (100% conversion)	1286:1318	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	80	theme	short	1238:1242	arg1	reusability					1416:1426	reusability	1416:1426	reusability	1416:1426	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	80	theme	short	1238:1242	arg1	times					1253:1257	The short reaction times	1234:1257	The short reaction times	1234:1257	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	80	theme	short	1238:1242	arg1	advantages					1436:1445	the advantages	1432:1445	the advantages of this method	1432:1460	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	6	80	theme	short	1238:1242	arg1	conditions					1274:1283	mild reaction conditions	1260:1283	mild reaction conditions	1260:1283	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	2	81	theme	nanocatalyst	490:501	arg1	properties					450:459	The physicochemical properties	430:459	The physicochemical properties of the microporous Pd-CS-CAC nanocatalyst developed	430:511	The physicochemical properties of the microporous Pd-CS-CAC nanocatalyst developed were successfully determined by FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques.					
36574802	0	82	theme	palladium	27:35	arg1	catalyst					37:44	Nano-sized and microporous palladium catalyst	0:44	Nano-sized and microporous palladium catalyst	0:44	Nano-sized and microporous palladium catalyst supported on modified chitosan/cigarette butt composite for treatment of environmental contaminants.					
36574802	1	83	theme	resistant	323:331	arg1	support					342:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support	263:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	263:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
36574802	8	84	theme	initial	1704:1710	arg1	shape					1712:1716	the initial shape	1700:1716	the initial shape	1700:1716	Moreover, Pd-CS-CAC was reused for 5 consecutive times with no loss of the initial shape, size, and morphology, confirming that it was a sustainable and robust nanocatalyst.					
36574802	2	85	theme	microporous	468:478	arg1	nanocatalyst					490:501	the microporous Pd-CS-CAC nanocatalyst	464:501	the microporous Pd-CS-CAC nanocatalyst developed	464:511	The physicochemical properties of the microporous Pd-CS-CAC nanocatalyst developed were successfully determined by FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques.					
36574802	3	86	theme	NPs	666:668	arg1	size					639:642	the average particle size	618:642	the average particle size of the synthesized Pd NPs	618:668	TEM studies showed that the average particle size of the synthesized Pd NPs was about 30 nm.					
36574802	5	87	theme	supernatant	1195:1205	arg1	spectroscopy					1175:1186	UV-Vis absorption spectroscopy	1157:1186	UV-Vis absorption spectroscopy of the supernatant at regular time intervals	1157:1231	The catalytic activities were studied by UV-Vis absorption spectroscopy of the supernatant at regular time intervals.					
36574802	3	88	theme	synthesized	651:661	arg1	NPs					666:668	the synthesized Pd NPs	647:668	the synthesized Pd NPs	647:668	TEM studies showed that the average particle size of the synthesized Pd NPs was about 30 nm.					
36574802	2	89	theme	physicochemical	434:448	arg1	properties					450:459	The physicochemical properties	430:459	The physicochemical properties of the microporous Pd-CS-CAC nanocatalyst developed	430:511	The physicochemical properties of the microporous Pd-CS-CAC nanocatalyst developed were successfully determined by FTIR, XRD, FE-SEM, TEM, BET, and EDS techniques.					
36574802	0	90	theme	modified	59:66	arg1	composite					92:100	modified chitosan/cigarette butt composite	59:100	modified chitosan/cigarette butt composite for treatment of environmental contaminants	59:144	Nano-sized and microporous palladium catalyst supported on modified chitosan/cigarette butt composite for treatment of environmental contaminants.					
36574802	6	91	theme	excellent	1342:1350	arg1	stability					1361:1369	excellent chemical stability	1342:1369	excellent chemical stability	1342:1369	The short reaction times, mild reaction conditions, high efficiency (100% conversion), easy separation, and excellent chemical stability of the catalyst due to its heterogeneity and reusability are the advantages of this method.					
36574802	1	92	theme	spherical	271:279	arg1	support					342:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support	263:348	a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC)	263:427	This study reports a versatile process for the fabrication of a microporous heterogeneous palladium nanocatalyst on a novel spherical, biodegradable, and chemically/physically resistant catalyst support consisting of chitosan (CS) and cigarette waste-derived activated carbon (CAC).					
35429671	9	0	theme	silver-loaded	2575:2587	arg1	microparticles					2589:2602	silver-loaded microparticles	2575:2602	silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size)	2575:2673	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	8	1	theme	bone	2173:2176	arg1	cement					2178:2183	bone cement	2173:2183	bone cement	2173:2183	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	4	2	theme	main	1051:1054	arg1	species					1056:1062	the main species	1047:1062	the main species involved in bone-implant infection (S. aureus and S. epidermidis)	1047:1128	A dose-dependent efficiency of silver was demonstrated on the main species involved in bone-implant infection (S. aureus and S. epidermidis).					
35429671	3	3	theme	step-by-step	679:690	arg1	methodology					692:702	an original step-by-step methodology	667:702	an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium	667:846	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	9	4	theme	encapsulation	2640:2652	arg1	requirements					2626:2637	fulfill requirements	2618:2637	fulfill requirements (encapsulation efficiency and size)	2618:2673	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	9	4	theme	encapsulation	2640:2652	arg1	efficiency					2654:2663	encapsulation efficiency	2640:2663	encapsulation efficiency	2640:2663	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	2	5	theme	calcium	621:627	arg1	cement					644:649	a self-setting calcium phosphate bone cement	606:649	a self-setting calcium phosphate bone cement	606:649	In view of controlling its delivery in situ at the desired dose, we investigated its encapsulation in carboxymethyl cellulose (CMC) microparticles by spray-drying and included the latter in the formulation of a self-setting calcium phosphate bone cement.					
35429671	7	6	theme	versatile	1792:1800	arg1	material					1818:1825	a versatile bone substitute material	1790:1825	a versatile bone substitute material with tunable drug delivery properties	1790:1863	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	9	7	theme	biological	2399:2408	arg1	tests					2410:2414	(micro)biological tests	2392:2414	(micro)biological tests on two silver salts with different solubilities	2392:2462	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	6	8	theme	CMC	1525:1527	arg1	microspheres					1529:1540	about 10% (w/w) silver-loaded CMC microspheres	1495:1540	about 10% (w/w) silver-loaded CMC microspheres	1495:1540	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	6	9	dep	silver-loaded	1511:1523	arg1	%					1503:1503	about 10%	1495:1503	about 10%	1495:1503	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	3	10	theme	antibacterial	744:756	arg1	properties					758:767	antibacterial properties	744:767	antibacterial properties	744:767	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	4	11	dep	infection	1089:1097	arg1	aureus					1103:1108	S. aureus	1100:1108	S. aureus	1100:1108	A dose-dependent efficiency of silver was demonstrated on the main species involved in bone-implant infection (S. aureus and S. epidermidis).					
35429671	4	11	dep	infection	1089:1097	arg1	epidermidis					1117:1127	S. epidermidis	1114:1127	S. epidermidis	1114:1127	A dose-dependent efficiency of silver was demonstrated on the main species involved in bone-implant infection (S. aureus and S. epidermidis).					
35429671	7	12	theme	cement	1759:1764	arg1	formulation					1766:1776	This injectable silver-loaded biomimetic composite cement formulation	1708:1776	This injectable silver-loaded biomimetic composite cement formulation	1708:1776	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	8	13	theme	release	2217:2223	arg1	properties					2225:2234	release properties	2217:2234	release properties	2217:2234	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	0	14	theme	infection	135:143	arg1	risk					145:148	implant-associated infection risk	116:148	implant-associated infection risk	116:148	Injectable bone cement containing carboxymethyl cellulose microparticles as a silver delivery system able to reduce implant-associated infection risk.					
35429671	1	15	theme	multiresistant	372:385	arg1	bacteria					387:394	planktonic, sessile as well as multiresistant bacteria	341:394	bacteria	387:394	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	9	16	theme	silver	2423:2428	arg1	salts					2430:2434	two silver salts	2419:2434	two silver salts with different solubilities	2419:2462	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	8	17	theme	SIGNIFICANCE	1936:1947	arg1	STATEMENT					1923:1931	STATEMENT	1923:1931	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose	1923:2144	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	1	18	theme	implant-associated	213:230	arg1	infections					232:241	implant-associated infections	213:241	implant-associated infections	213:241	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	8	19	dep	Choice	2036:2041	arg1	i					2033:2033	i	2033:2033	i	2033:2033	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	9	20	dep	requirements	2626:2637	arg1	requirements					2626:2637	fulfill requirements	2618:2637	fulfill requirements (encapsulation efficiency and size)	2618:2673	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	9	20	dep	requirements	2626:2637	arg1	efficiency					2654:2663	encapsulation efficiency	2640:2663	encapsulation efficiency	2640:2663	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	9	20	dep	requirements	2626:2637	arg1	size					2669:2672	size	2669:2672	size	2669:2672	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	7	21	theme	silver-loaded	1724:1736	arg1	formulation					1766:1776	This injectable silver-loaded biomimetic composite cement formulation	1708:1776	This injectable silver-loaded biomimetic composite cement formulation	1708:1776	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	8	22	theme	innovative	1977:1986	arg1	aspects					1999:2005	two innovative scientific aspects	1973:2005	two innovative scientific aspects regarding the literature	1973:2030	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	10	23	theme	active	2747:2752	arg1	dose					2754:2757	active dose	2747:2757	active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections	2747:2852	The obtained fully injectable composite controls the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections.					
35429671	3	24	theme	silver	793:798	arg1	salts					800:804	two silver salts	789:804	two silver salts of different solubility in aqueous medium	789:846	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	6	25	theme	cement	1549:1554	arg1	formulation					1556:1566	the cement formulation	1545:1566	the cement formulation	1545:1566	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	1	26	theme	challenging	158:168	arg1	quest					170:174	the challenging quest	154:174	the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery	154:270	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	3	27	theme	aqueous	833:839	arg1	medium					841:846	aqueous medium	833:846	aqueous medium	833:846	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	0	28	theme	delivery	85:92	arg1	system					94:99	a silver delivery system	76:99	a silver delivery system able to reduce implant-associated infection risk	76:148	Injectable bone cement containing carboxymethyl cellulose microparticles as a silver delivery system able to reduce implant-associated infection risk.					
35429671	1	29	theme	planktonic	341:350	arg1	bacteria					387:394	planktonic, sessile as well as multiresistant bacteria	341:394	bacteria	387:394	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	9	30	theme	silver	2487:2492	arg1	dose					2494:2497	silver dose	2487:2497	silver dose	2487:2497	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	1	31	from	quest	170:174	arg1	promising					299:307	promising	299:307	promising	299:307	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	1	31	from	quest	170:174	arg1	use					277:279	the use	273:279	the use of silver ions	273:294	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	10	32	from	dose	2754:2757	arg1	3 h					2775:2777	3 h	2775:2777	3 h	2775:2777	The obtained fully injectable composite controls the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections.					
35429671	10	33	theme	early	2729:2733	arg1	delivery					2735:2742	the early delivery	2725:2742	the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections	2725:2852	The obtained fully injectable composite controls the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections.					
35429671	2	34	theme	cement	644:649	arg1	formulation					591:601	the formulation	587:601	the formulation of a self-setting calcium phosphate bone cement	587:649	In view of controlling its delivery in situ at the desired dose, we investigated its encapsulation in carboxymethyl cellulose (CMC) microparticles by spray-drying and included the latter in the formulation of a self-setting calcium phosphate bone cement.					
35429671	3	35	theme	solubility	819:828	arg1	salts					800:804	two silver salts	789:804	two silver salts of different solubility in aqueous medium	789:846	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	0	36	theme	able	101:104	arg1	system					94:99	a silver delivery system	76:99	a silver delivery system able to reduce implant-associated infection risk	76:148	Injectable bone cement containing carboxymethyl cellulose microparticles as a silver delivery system able to reduce implant-associated infection risk.					
35429671	3	37	theme	silver	897:902	arg1	loading					904:910	silver loading	897:910	silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial	897:986	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	2	38	theme	carboxymethyl	499:511	arg1	microparticles					529:542	carboxymethyl cellulose (CMC) microparticles	499:542	carboxymethyl cellulose (CMC) microparticles	499:542	In view of controlling its delivery in situ at the desired dose, we investigated its encapsulation in carboxymethyl cellulose (CMC) microparticles by spray-drying and included the latter in the formulation of a self-setting calcium phosphate bone cement.					
35429671	2	39	theme	phosphate	629:637	arg1	cement					644:649	a self-setting calcium phosphate bone cement	606:649	a self-setting calcium phosphate bone cement	606:649	In view of controlling its delivery in situ at the desired dose, we investigated its encapsulation in carboxymethyl cellulose (CMC) microparticles by spray-drying and included the latter in the formulation of a self-setting calcium phosphate bone cement.					
35429671	9	40	theme	antibacterial	2269:2281	arg1	cement					2303:2308	an antibacterial and injectable bone cement	2266:2308	an antibacterial and injectable bone cement	2266:2308	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	8	41	theme	antibacterial	2061:2073	arg1	agent					2075:2079	antibacterial agent	2061:2079	antibacterial agent combined with their way of incorporation	2061:2120	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	9	42	theme	injectable	2287:2296	arg1	cement					2303:2308	an antibacterial and injectable bone cement	2266:2308	an antibacterial and injectable bone cement	2266:2308	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	0	43	theme	Injectable	0:9	arg1	cement					16:21	Injectable bone cement	0:21	Injectable bone cement containing carboxymethyl cellulose	0:56	Injectable bone cement containing carboxymethyl cellulose microparticles as a silver delivery system able to reduce implant-associated infection risk.					
35429671	6	44	theme	injectable	1604:1613	arg1	cement					1639:1644	a fully injectable and highly porous (77%) cement	1596:1644	a fully injectable and highly porous (77%) cement	1596:1644	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	3	45	from	solubility	819:828	arg1	medium					841:846	aqueous medium	833:846	aqueous medium	833:846	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	5	46	theme	silver	1313:1318	arg1	release					1302:1308	a faster release	1293:1308	a faster release	1293:1308	Loading silver in microspheres instead of loading it directly inside the cement permitted to avoid undesired silver-cement interactions during setting and led to a faster release of silver, i.e. to a higher dose released within the first days combining anti-biofilm activity and preserved cytocompatibility.					
35429671	1	47	theme	ions	291:294	arg1	promising					299:307	promising	299:307	promising	299:307	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	1	47	theme	ions	291:294	arg1	use					277:279	the use	273:279	the use of silver ions	273:294	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	3	48	theme	able	912:915	arg1	loading					904:910	silver loading	897:910	silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial	897:986	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	10	49	theme	able	2797:2800	arg1	silver					2762:2767	silver	2762:2767	silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections	2762:2852	The obtained fully injectable composite controls the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections.					
35429671	10	50	theme	obtained	2680:2687	arg1	composite					2706:2714	The obtained fully injectable composite	2676:2714	The obtained fully injectable composite	2676:2714	The obtained fully injectable composite controls the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections.					
35429671	9	51	theme	original	2311:2318	arg1	methodology					2355:2365	original and multidisciplinary step-by-step methodology	2311:2365	methodology	2355:2365	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	0	52	theme	carboxymethyl	34:46	arg1	cellulose					48:56	carboxymethyl cellulose	34:56	carboxymethyl cellulose	34:56	Injectable bone cement containing carboxymethyl cellulose microparticles as a silver delivery system able to reduce implant-associated infection risk.					
35429671	5	53	from	silver	1139:1144	arg1	microspheres					1149:1160	microspheres	1149:1160	microspheres	1149:1160	Loading silver in microspheres instead of loading it directly inside the cement permitted to avoid undesired silver-cement interactions during setting and led to a faster release of silver, i.e. to a higher dose released within the first days combining anti-biofilm activity and preserved cytocompatibility.					
35429671	4	54	theme	silver	1020:1025	arg1	efficiency					1006:1015	A dose-dependent efficiency	989:1015	A dose-dependent efficiency of silver	989:1025	A dose-dependent efficiency of silver was demonstrated on the main species involved in bone-implant infection (S. aureus and S. epidermidis).					
35429671	9	55	theme	multidisciplinary	2324:2340	arg1	methodology					2355:2365	original and multidisciplinary step-by-step methodology	2311:2365	methodology	2355:2365	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	7	56	theme	associated	1901:1910	arg1	infection					1912:1920	bone implant associated infection	1888:1920	bone implant associated infection	1888:1920	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	7	57	theme	bone	1888:1891	arg1	infection					1912:1920	bone implant associated infection	1888:1920	bone implant associated infection	1888:1920	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	7	58	theme	delivery	1845:1852	arg1	properties					1854:1863	tunable drug delivery properties	1832:1863	tunable drug delivery properties	1832:1863	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	10	59	theme	bone	2819:2822	arg1	infections					2843:2852	bone implant-associated infections	2819:2852	bone implant-associated infections	2819:2852	The obtained fully injectable composite controls the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections.					
35429671	3	60	theme	original	670:677	arg1	methodology					692:702	an original step-by-step methodology	667:702	an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium	667:846	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	1	61	theme	infections	232:241	arg1	risk					205:208	the risk	201:208	the risk of implant-associated infections in bone substitution surgery	201:270	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	7	62	theme	tunable	1832:1838	arg1	properties					1854:1863	tunable drug delivery properties	1832:1863	tunable drug delivery properties	1832:1863	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	10	63	theme	injectable	2695:2704	arg1	composite					2706:2714	The obtained fully injectable composite	2676:2714	The obtained fully injectable composite	2676:2714	The obtained fully injectable composite controls the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections.					
35429671	6	64	theme	introduction	1479:1490	arg1	interest					1463:1470	a combined interest	1452:1470	a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation	1452:1566	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	5	65	theme	silver-cement	1240:1252	arg1	interactions					1254:1265	undesired silver-cement interactions	1230:1265	undesired silver-cement interactions	1230:1265	Loading silver in microspheres instead of loading it directly inside the cement permitted to avoid undesired silver-cement interactions during setting and led to a faster release of silver, i.e. to a higher dose released within the first days combining anti-biofilm activity and preserved cytocompatibility.					
35429671	2	66	theme	desired	448:454	arg1	dose					456:459	the desired dose	444:459	the desired dose	444:459	In view of controlling its delivery in situ at the desired dose, we investigated its encapsulation in carboxymethyl cellulose (CMC) microparticles by spray-drying and included the latter in the formulation of a self-setting calcium phosphate bone cement.					
35429671	5	67	theme	first	1363:1367	arg1	days					1369:1372	the first days	1359:1372	the first days combining anti-biofilm activity and preserved cytocompatibility	1359:1436	Loading silver in microspheres instead of loading it directly inside the cement permitted to avoid undesired silver-cement interactions during setting and led to a faster release of silver, i.e. to a higher dose released within the first days combining anti-biofilm activity and preserved cytocompatibility.					
35429671	7	68	theme	bone	1802:1805	arg1	material					1818:1825	a versatile bone substitute material	1790:1825	a versatile bone substitute material with tunable drug delivery properties	1790:1863	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	9	69	dep	microparticles	2589:2602	arg1	processing					2604:2613	processing	2604:2613	processing	2604:2613	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	0	70	contain	containing	23:32	arg1	cement					16:21	Injectable bone cement	0:21	Injectable bone cement containing carboxymethyl cellulose	0:56	Injectable bone cement containing carboxymethyl cellulose microparticles as a silver delivery system able to reduce implant-associated infection risk.					
35429671	0	70	contain	containing	23:32	arg2	cellulose					48:56	carboxymethyl cellulose	34:56	carboxymethyl cellulose	34:56	Injectable bone cement containing carboxymethyl cellulose microparticles as a silver delivery system able to reduce implant-associated infection risk.					
35429671	1	71	from	promising	299:307	arg1	quest					170:174	the challenging quest	154:174	the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery	154:270	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	6	72	theme	combined	1454:1461	arg1	interest					1463:1470	a combined interest	1452:1470	a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation	1452:1566	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	6	73	theme	microspheres	1529:1540	arg1	introduction					1479:1490	the introduction	1475:1490	the introduction of about 10% (w/w) silver-loaded CMC microspheres	1475:1540	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	8	74	theme	ions	2053:2056	arg1	Choice					2036:2041	Choice	2036:2041	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose	1923:2144	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	2	75	theme	self-setting	608:619	arg1	cement					644:649	a self-setting calcium phosphate bone cement	606:649	a self-setting calcium phosphate bone cement	606:649	In view of controlling its delivery in situ at the desired dose, we investigated its encapsulation in carboxymethyl cellulose (CMC) microparticles by spray-drying and included the latter in the formulation of a self-setting calcium phosphate bone cement.					
35429671	3	76	theme	in	722:723	arg1	study					731:735	the in vitro study	718:735	the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium	718:846	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	5	77	theme	preserved	1410:1418	arg1	cytocompatibility					1420:1436	preserved cytocompatibility	1410:1436	preserved cytocompatibility	1410:1436	Loading silver in microspheres instead of loading it directly inside the cement permitted to avoid undesired silver-cement interactions during setting and led to a faster release of silver, i.e. to a higher dose released within the first days combining anti-biofilm activity and preserved cytocompatibility.					
35429671	6	78	theme	silver-loaded	1511:1523	arg1	microspheres					1529:1540	about 10% (w/w) silver-loaded CMC microspheres	1495:1540	about 10% (w/w) silver-loaded CMC microspheres	1495:1540	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	5	79	theme	Loading	1131:1137	arg1	silver					1139:1144	Loading silver	1131:1144	Loading silver in microspheres instead of loading it	1131:1182	Loading silver in microspheres instead of loading it directly inside the cement permitted to avoid undesired silver-cement interactions during setting and led to a faster release of silver, i.e. to a higher dose released within the first days combining anti-biofilm activity and preserved cytocompatibility.					
35429671	7	80	theme	composite	1749:1757	arg1	formulation					1766:1776	This injectable silver-loaded biomimetic composite cement formulation	1708:1776	This injectable silver-loaded biomimetic composite cement formulation	1708:1776	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	8	81	theme	drug	2200:2203	arg1	loading					2205:2211	its drug loading	2196:2211	its drug loading	2196:2211	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	7	82	dep	material	1818:1825	arg1	substitute					1807:1816	substitute	1807:1816	substitute	1807:1816	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	3	83	theme	properties	758:767	arg1	study					731:735	the in vitro study	718:735	the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium	718:846	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	6	84	theme	analogous	1678:1686	arg1	strength					1669:1676	a compressive strength	1655:1676	a compressive strength analogous to cancellous bone	1655:1705	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	9	85	theme	step-by-step	2342:2353	arg1	methodology					2355:2365	original and multidisciplinary step-by-step methodology	2311:2365	methodology	2355:2365	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	2	86	theme	CMC	524:526	arg1	microparticles					529:542	carboxymethyl cellulose (CMC) microparticles	499:542	carboxymethyl cellulose (CMC) microparticles	499:542	In view of controlling its delivery in situ at the desired dose, we investigated its encapsulation in carboxymethyl cellulose (CMC) microparticles by spray-drying and included the latter in the formulation of a self-setting calcium phosphate bone cement.					
35429671	2	87	from	encapsulation	482:494	arg1	microparticles					529:542	carboxymethyl cellulose (CMC) microparticles	499:542	carboxymethyl cellulose (CMC) microparticles	499:542	In view of controlling its delivery in situ at the desired dose, we investigated its encapsulation in carboxymethyl cellulose (CMC) microparticles by spray-drying and included the latter in the formulation of a self-setting calcium phosphate bone cement.					
35429671	9	88	dep	include	2502:2508	arg1	optimization					2559:2570	optimization	2559:2570	optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size)	2559:2673	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	9	89	theme	different	2441:2449	arg1	solubilities					2451:2462	different solubilities	2441:2462	different solubilities	2441:2462	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	10	90	theme	silver	2762:2767	arg1	dose					2754:2757	active dose	2747:2757	active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections	2747:2852	The obtained fully injectable composite controls the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections.					
35429671	3	91	theme	cytotoxicity	773:784	arg1	study					731:735	the in vitro study	718:735	the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium	718:846	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	1	92	from	risk	205:208	arg1	surgery					264:270	bone substitution surgery	246:270	bone substitution surgery	246:270	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	7	93	theme	biomimetic	1738:1747	arg1	formulation					1766:1776	This injectable silver-loaded biomimetic composite cement formulation	1708:1776	This injectable silver-loaded biomimetic composite cement formulation	1708:1776	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	10	94	theme	dose	2754:2757	arg1	delivery					2735:2742	the early delivery	2725:2742	the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections	2725:2852	The obtained fully injectable composite controls the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections.					
35429671	5	95	theme	faster	1295:1300	arg1	release					1302:1308	a faster release	1293:1308	a faster release	1293:1308	Loading silver in microspheres instead of loading it directly inside the cement permitted to avoid undesired silver-cement interactions during setting and led to a faster release of silver, i.e. to a higher dose released within the first days combining anti-biofilm activity and preserved cytocompatibility.					
35429671	3	96	theme	different	809:817	arg1	solubility					819:828	different solubility	809:828	different solubility in aqueous medium	809:846	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	0	97	theme	silver	78:83	arg1	system					94:99	a silver delivery system	76:99	a silver delivery system able to reduce implant-associated infection risk	76:148	Injectable bone cement containing carboxymethyl cellulose microparticles as a silver delivery system able to reduce implant-associated infection risk.					
35429671	9	98	theme	targeted	2469:2476	arg1	range					2478:2482	the targeted range	2465:2482	the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size)	2465:2673	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	7	99	theme	injectable	1713:1722	arg1	formulation					1766:1776	This injectable silver-loaded biomimetic composite cement formulation	1708:1776	This injectable silver-loaded biomimetic composite cement formulation	1708:1776	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	3	100	theme	salts	800:804	arg1	cytotoxicity					773:784	cytotoxicity	773:784	cytotoxicity	773:784	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	3	100	theme	salts	800:804	arg1	properties					758:767	antibacterial properties	744:767	antibacterial properties	744:767	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	8	101	theme	scientific	1988:1997	arg1	aspects					1999:2005	two innovative scientific aspects	1973:2005	two innovative scientific aspects regarding the literature	1973:2030	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	5	102	dep	loading	1173:1179	arg1	it					1181:1182	it	1181:1182	it	1181:1182	Loading silver in microspheres instead of loading it directly inside the cement permitted to avoid undesired silver-cement interactions during setting and led to a faster release of silver, i.e. to a higher dose released within the first days combining anti-biofilm activity and preserved cytocompatibility.					
35429671	3	103	dep	properties	758:767	arg1	the					740:742	the	740:742	the	740:742	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	9	104	theme	dose	2494:2497	arg1	range					2478:2482	the targeted range	2465:2482	the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size)	2465:2673	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	2	105	theme	cellulose	513:521	arg1	microparticles					529:542	carboxymethyl cellulose (CMC) microparticles	499:542	carboxymethyl cellulose (CMC) microparticles	499:542	In view of controlling its delivery in situ at the desired dose, we investigated its encapsulation in carboxymethyl cellulose (CMC) microparticles by spray-drying and included the latter in the formulation of a self-setting calcium phosphate bone cement.					
35429671	2	106	theme	bone	639:642	arg1	cement					644:649	a self-setting calcium phosphate bone cement	606:649	a self-setting calcium phosphate bone cement	606:649	In view of controlling its delivery in situ at the desired dose, we investigated its encapsulation in carboxymethyl cellulose (CMC) microparticles by spray-drying and included the latter in the formulation of a self-setting calcium phosphate bone cement.					
35429671	0	107	theme	implant-associated	116:133	arg1	risk					145:148	implant-associated infection risk	116:148	implant-associated infection risk	116:148	Injectable bone cement containing carboxymethyl cellulose microparticles as a silver delivery system able to reduce implant-associated infection risk.					
35429671	6	108	theme	porous	1626:1631	arg1	cement					1639:1644	a fully injectable and highly porous (77%) cement	1596:1644	a fully injectable and highly porous (77%) cement	1596:1644	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	3	109	theme	loading	904:910	arg1	range					888:892	the range	884:892	the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial	884:986	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	6	110	theme	cancellous	1691:1700	arg1	bone					1702:1705	cancellous bone	1691:1705	cancellous bone	1691:1705	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	5	111	theme	higher	1331:1336	arg1	dose					1338:1341	a higher dose	1329:1341	a higher dose released within the first days combining anti-biofilm activity and preserved cytocompatibility	1329:1436	Loading silver in microspheres instead of loading it directly inside the cement permitted to avoid undesired silver-cement interactions during setting and led to a faster release of silver, i.e. to a higher dose released within the first days combining anti-biofilm activity and preserved cytocompatibility.					
35429671	9	112	with	salts	2430:2434	arg1	solubilities					2451:2462	different solubilities	2441:2462	different solubilities	2441:2462	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	0	113	theme	bone	11:14	arg1	cement					16:21	Injectable bone cement	0:21	Injectable bone cement containing carboxymethyl cellulose	0:56	Injectable bone cement containing carboxymethyl cellulose microparticles as a silver delivery system able to reduce implant-associated infection risk.					
35429671	9	114	theme	carboxymethylcellulose	2513:2534	arg1	microspheres					2536:2547	carboxymethylcellulose microspheres	2513:2547	carboxymethylcellulose microspheres	2513:2547	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	9	115	theme	bone	2298:2301	arg1	cement					2303:2308	an antibacterial and injectable bone cement	2266:2308	an antibacterial and injectable bone cement	2266:2308	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	1	116	theme	silver	284:289	arg1	ions					291:294	silver ions	284:294	silver ions	284:294	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	6	117	dep	porous	1626:1631	arg1	%					1636:1636	77%	1634:1636	77%	1634:1636	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	10	118	from	3 h	2775:2777	arg1	dose					2754:2757	active dose	2747:2757	active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections	2747:2852	The obtained fully injectable composite controls the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections.					
35429671	10	118	from	3 h	2775:2777	arg1	silver					2762:2767	silver	2762:2767	silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections	2762:2852	The obtained fully injectable composite controls the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections.					
35429671	8	119	theme	silver	2046:2051	arg1	ions					2053:2056	silver ions	2046:2056	silver ions as antibacterial agent combined with their way of incorporation	2046:2120	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	7	120	theme	implant	1893:1899	arg1	infection					1912:1920	bone implant associated infection	1888:1920	bone implant associated infection	1888:1920	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	8	121	theme	incorporation	2108:2120	arg1	way					2101:2103	their way	2095:2103	their way of incorporation	2095:2120	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	4	122	theme	dose-dependent	991:1004	arg1	efficiency					1006:1015	A dose-dependent efficiency	989:1015	A dose-dependent efficiency of silver	989:1025	A dose-dependent efficiency of silver was demonstrated on the main species involved in bone-implant infection (S. aureus and S. epidermidis).					
35429671	3	123	from	medium	841:846	arg1	salts					800:804	two silver salts	789:804	two silver salts of different solubility in aqueous medium	789:846	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	1	124	theme	bone	246:249	arg1	surgery					264:270	bone substitution surgery	246:270	bone substitution surgery	246:270	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	1	125	from	spectrum	329:336	arg1	bacteria					387:394	planktonic, sessile as well as multiresistant bacteria	341:394	bacteria	387:394	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	1	125	from	spectrum	329:336	arg1	sessile					353:359	planktonic, sessile as well as multiresistant bacteria	341:394	sessile	353:359	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	3	126	dep	in	722:723	arg1	vitro					725:729	vitro	725:729	vitro	725:729	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	9	127	theme	fulfill	2618:2624	arg1	requirements					2626:2637	fulfill requirements	2618:2637	fulfill requirements (encapsulation efficiency and size)	2618:2673	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	9	127	theme	fulfill	2618:2624	arg1	efficiency					2654:2663	encapsulation efficiency	2640:2663	encapsulation efficiency	2640:2663	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	9	127	theme	fulfill	2618:2624	arg1	size					2669:2672	size	2669:2672	size	2669:2672	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	8	128	dep	STATEMENT	1923:1931	arg1	Choice					2036:2041	Choice	2036:2041	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose	1923:2144	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	8	128	dep	STATEMENT	1923:1931	arg1	Carboxymethylcellulose					2123:2144	Carboxymethylcellulose	2123:2144	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose	1923:2144	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	8	128	dep	STATEMENT	1923:1931	arg1	based					1964:1968	based	1964:1968	is based on two innovative scientific aspects regarding the literature	1961:2030	STATEMENT OF SIGNIFICANCE: This study is based on two innovative scientific aspects regarding the literature: i) Choice of silver ions as antibacterial agent combined with their way of incorporation: Carboxymethylcellulose has never been tested into bone cement to control its drug loading and release properties.					
35429671	10	129	theme	implant-associated	2824:2841	arg1	infections					2843:2852	bone implant-associated infections	2819:2852	bone implant-associated infections	2819:2852	The obtained fully injectable composite controls the early delivery of active dose of silver (from 3 h and over 2 weeks) able to fight against bone implant-associated infections.					
35429671	3	130	from	salts	800:804	arg1	medium					841:846	aqueous medium	833:846	aqueous medium	833:846	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	1	131	dep	solution	182:189	arg1	reduce					194:199	reduce	194:199	to reduce the risk of implant-associated infections in bone substitution surgery	191:270	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	5	132	theme	anti-biofilm	1384:1395	arg1	activity					1397:1404	anti-biofilm activity	1384:1404	anti-biofilm activity	1384:1404	Loading silver in microspheres instead of loading it directly inside the cement permitted to avoid undesired silver-cement interactions during setting and led to a faster release of silver, i.e. to a higher dose released within the first days combining anti-biofilm activity and preserved cytocompatibility.					
35429671	6	133	theme	compressive	1657:1667	arg1	strength					1669:1676	a compressive strength	1655:1676	a compressive strength analogous to cancellous bone	1655:1705	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	1	134	theme	broad	323:327	arg1	spectrum					329:336	its broad spectrum	319:336	its broad spectrum on planktonic, sessile as well as multiresistant bacteria	319:394	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
35429671	9	135	theme	microparticles	2589:2602	arg1	optimization					2559:2570	optimization	2559:2570	optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size)	2559:2673	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	6	136	from	interest	1463:1470	arg1	formulation					1556:1566	the cement formulation	1545:1566	the cement formulation	1545:1566	In addition, a combined interest of the introduction of about 10% (w/w) silver-loaded CMC microspheres in the cement formulation was demonstrated leading to a fully injectable and highly porous (77%) cement, showing a compressive strength analogous to cancellous bone.					
35429671	3	137	theme	non-cytotoxic	944:956	arg1	properties					958:967	non-cytotoxic properties	944:967	non-cytotoxic properties	944:967	We implemented an original step-by-step methodology starting from the in vitro study of the antibacterial properties and cytotoxicity of two silver salts of different solubility in aqueous medium and then in the cement to determine the range of silver loading able to confer anti-biofilm and non-cytotoxic properties to the biomaterial.					
35429671	4	138	theme	bone-implant	1076:1087	arg1	infection					1089:1097	bone-implant infection	1076:1097	bone-implant infection (S. aureus and S. epidermidis)	1076:1128	A dose-dependent efficiency of silver was demonstrated on the main species involved in bone-implant infection (S. aureus and S. epidermidis).					
35429671	7	139	with	material	1818:1825	arg1	properties					1854:1863	tunable drug delivery properties	1832:1863	tunable drug delivery properties	1832:1863	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	9	140	from	tests	2410:2414	arg1	salts					2430:2434	two silver salts	2419:2434	two silver salts with different solubilities	2419:2462	ii) Methodology to formulate an antibacterial and injectable bone cement: original and multidisciplinary step-by-step methodology to first define, through (micro)biological tests on two silver salts with different solubilities, the targeted range of silver dose to include in carboxymethylcellulose microspheres and, then optimization of silver-loaded microparticles processing to fulfill requirements (encapsulation efficiency and size).					
35429671	5	141	theme	undesired	1230:1238	arg1	interactions					1254:1265	undesired silver-cement interactions	1230:1265	undesired silver-cement interactions	1230:1265	Loading silver in microspheres instead of loading it directly inside the cement permitted to avoid undesired silver-cement interactions during setting and led to a faster release of silver, i.e. to a higher dose released within the first days combining anti-biofilm activity and preserved cytocompatibility.					
35429671	7	142	theme	drug	1840:1843	arg1	properties					1854:1863	tunable drug delivery properties	1832:1863	tunable drug delivery properties	1832:1863	This injectable silver-loaded biomimetic composite cement formulation constitutes a versatile bone substitute material with tunable drug delivery properties, able to fight against bone implant associated infection.					
35429671	1	143	theme	substitution	251:262	arg1	surgery					264:270	bone substitution surgery	246:270	bone substitution surgery	246:270	In the challenging quest for a solution to reduce the risk of implant-associated infections in bone substitution surgery, the use of silver ions is promising regarding its broad spectrum on planktonic, sessile as well as multiresistant bacteria.					
37170624	7	0	theme	gut	955:957	arg1	microbiota					959:968	the gut microbiota	951:968	the gut microbiota	951:968	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	2	1	theme	simulated	364:372	arg1	digestion					374:382	the simulated digestion	360:382	the simulated digestion under saliva, gastric, and small intestinal conditions	360:437	The results indicated that the simulated digestion under saliva, gastric, and small intestinal conditions had no effect on GL1-E1 and GL1-E2.					
37170624	7	2	theme	Bacteroides	1012:1022	arg1	abundances					998:1007	the relative abundances	985:1007	the relative abundances of Bacteroides and Phascolarctobacterium	985:1048	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	0	3	theme	Lacticaseibacillus	79:96	arg1	GL1					108:110	Lacticaseibacillus paracasei GL1	79:110	Lacticaseibacillus paracasei GL1	79:110	In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1.					
37170624	2	4	contain	had	439:441	arg1	digestion					374:382	the simulated digestion	360:382	the simulated digestion under saliva, gastric, and small intestinal conditions	360:437	The results indicated that the simulated digestion under saliva, gastric, and small intestinal conditions had no effect on GL1-E1 and GL1-E2.					
37170624	2	4	contain	had	439:441	arg2	effect					446:451	no effect	443:451	no effect	443:451	The results indicated that the simulated digestion under saliva, gastric, and small intestinal conditions had no effect on GL1-E1 and GL1-E2.					
37170624	3	5	theme	Lactococcus	555:565	arg1	growth					567:572	Lactococcus growth	555:572	Lactococcus growth	555:572	Additionally, GL1-E1 and GL1-E2 can be used as substrates for Lactobacillus and Lactococcus growth.					
37170624	1	6	theme	in	277:278	arg1	fermentation					286:297	in vitro fermentation	277:297	in vitro fermentation of pure and human fecal cultures	277:330	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	7	7	theme	microbiota	959:968	arg1	composition					936:946	the composition	932:946	the composition of the gut microbiota	932:968	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	6	8	theme	acid	866:869	arg1	SCFA					831:834	the major SCFA	821:834	the major SCFA of formic, lactic, and acetic acid	821:869	Additionally, the total short-chain fatty acid (SCFA) production significantly increased, especially the major SCFA of formic, lactic, and acetic acid.					
37170624	7	9	theme	relative	989:996	arg1	abundances					998:1007	the relative abundances	985:1007	the relative abundances of Bacteroides and Phascolarctobacterium	985:1048	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	1	10	from	fractions	154:162	arg1	GL1					247:249	Lacticaseibacillus paracasei GL1	218:249	Lacticaseibacillus paracasei GL1	218:249	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	6	11	theme	acetic	859:864	arg1	acid					866:869	formic, lactic, and acetic acid	839:869	acid	866:869	Additionally, the total short-chain fatty acid (SCFA) production significantly increased, especially the major SCFA of formic, lactic, and acetic acid.					
37170624	4	12	theme	gut	647:649	arg1	microbiota					651:660	the gut microbiota	643:660	the gut microbiota	643:660	It was also found that both were gradually degraded and utilized by the gut microbiota.					
37170624	2	13	theme	gastric	398:404	arg1	saliva					390:395	saliva	390:395	saliva	390:395	The results indicated that the simulated digestion under saliva, gastric, and small intestinal conditions had no effect on GL1-E1 and GL1-E2.					
37170624	0	14	theme	In	0:1	arg1	digestion					19:27	In vitro simulated digestion	0:27	In vitro simulated digestion	0:27	In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1.					
37170624	7	15	theme	pathogenic	1070:1079	arg1	bacteria					1081:1088	the pathogenic bacteria	1066:1088	the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium	1066:1140	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	7	15	theme	pathogenic	1070:1079	arg1	Klebsiella					1112:1121	Klebsiella	1112:1121	Klebsiella	1112:1121	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	7	15	theme	pathogenic	1070:1079	arg1	Fusobacterium					1128:1140	Fusobacterium	1128:1140	Fusobacterium	1128:1140	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	7	15	theme	pathogenic	1070:1079	arg1	Escherichia-Shigella					1090:1109	Escherichia-Shigella	1090:1109	Escherichia-Shigella	1090:1109	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	0	16	dep	Lacticaseibacillus	79:96	arg1	paracasei					98:106	paracasei	98:106	paracasei	98:106	In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1.					
37170624	1	17	theme	exopolysaccharides	187:204	arg1	GL1-E2					176:181	GL1-E2	176:181	GL1-E2	176:181	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	17	theme	exopolysaccharides	187:204	arg1	GL1-E1					165:170	GL1-E1	165:170	GL1-E1	165:170	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	17	theme	exopolysaccharides	187:204	arg1	fractions					154:162	two purified fractions	141:162	two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1	141:249	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	6	18	theme	acid	762:765	arg1	production					774:783	the total short-chain fatty acid (SCFA) production	734:783	the total short-chain fatty acid (SCFA) production	734:783	Additionally, the total short-chain fatty acid (SCFA) production significantly increased, especially the major SCFA of formic, lactic, and acetic acid.					
37170624	1	19	dep	fractions	154:162	arg1	GL1-E2					176:181	GL1-E2	176:181	GL1-E2	176:181	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	19	dep	fractions	154:162	arg1	GL1-E1					165:170	GL1-E1	165:170	GL1-E1	165:170	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	19	dep	fractions	154:162	arg1	fractions					154:162	two purified fractions	141:162	two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1	141:249	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	0	20	theme	simulated	9:17	arg1	digestion					19:27	In vitro simulated digestion	0:27	In vitro simulated digestion	0:27	In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1.					
37170624	1	21	theme	pure	302:305	arg1	cultures					323:330	pure and human fecal cultures	302:330	cultures	323:330	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	6	22	theme	lactic	847:852	arg1	SCFA					831:834	the major SCFA	821:834	the major SCFA of formic, lactic, and acetic acid	821:869	Additionally, the total short-chain fatty acid (SCFA) production significantly increased, especially the major SCFA of formic, lactic, and acetic acid.					
37170624	1	23	theme	prebiotic	117:125	arg1	properties					127:136	The prebiotic properties	113:136	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1	113:249	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	2	24	theme	intestinal	417:426	arg1	conditions					428:437	saliva, gastric, and small intestinal conditions	390:437	saliva, gastric, and small intestinal conditions	390:437	The results indicated that the simulated digestion under saliva, gastric, and small intestinal conditions had no effect on GL1-E1 and GL1-E2.					
37170624	0	25	theme	fecal	33:37	arg1	fermentation					39:50	fecal fermentation	33:50	fecal fermentation	33:50	In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1.					
37170624	7	26	theme	Phascolarctobacterium	1028:1048	arg1	abundances					998:1007	the relative abundances	985:1007	the relative abundances of Bacteroides and Phascolarctobacterium	985:1048	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	2	27	theme	saliva	390:395	arg1	conditions					428:437	saliva, gastric, and small intestinal conditions	390:437	saliva, gastric, and small intestinal conditions	390:437	The results indicated that the simulated digestion under saliva, gastric, and small intestinal conditions had no effect on GL1-E1 and GL1-E2.					
37170624	1	28	theme	human	311:315	arg1	cultures					323:330	pure and human fecal cultures	302:330	cultures	323:330	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	29	dep	Lacticaseibacillus	218:235	arg1	paracasei					237:245	paracasei	237:245	paracasei	237:245	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	6	30	theme	fatty	756:760	arg1	SCFA					768:771	SCFA	768:771	SCFA	768:771	Additionally, the total short-chain fatty acid (SCFA) production significantly increased, especially the major SCFA of formic, lactic, and acetic acid.					
37170624	6	30	theme	fatty	756:760	arg1	acid					762:765	short-chain fatty acid	744:765	the total short-chain fatty acid (SCFA) production	734:783	Additionally, the total short-chain fatty acid (SCFA) production significantly increased, especially the major SCFA of formic, lactic, and acetic acid.					
37170624	7	31	dep	bacteria	1081:1088	arg1	bacteria					1081:1088	the pathogenic bacteria	1066:1088	the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium	1066:1140	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	7	31	dep	bacteria	1081:1088	arg1	Klebsiella					1112:1121	Klebsiella	1112:1121	Klebsiella	1112:1121	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	7	31	dep	bacteria	1081:1088	arg1	Fusobacterium					1128:1140	Fusobacterium	1128:1140	Fusobacterium	1128:1140	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	7	31	dep	bacteria	1081:1088	arg1	Escherichia-Shigella					1090:1109	Escherichia-Shigella	1090:1109	Escherichia-Shigella	1090:1109	Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium.					
37170624	1	32	theme	fecal	317:321	arg1	cultures					323:330	pure and human fecal cultures	302:330	cultures	323:330	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	33	from	GL1	247:249	arg1	EPSs					207:210	EPSs	207:210	EPSs	207:210	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	33	from	GL1	247:249	arg1	GL1-E2					176:181	GL1-E2	176:181	GL1-E2	176:181	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	33	from	GL1	247:249	arg1	exopolysaccharides					187:204	exopolysaccharides	187:204	exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1	187:249	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	33	from	GL1	247:249	arg1	GL1-E1					165:170	GL1-E1	165:170	GL1-E1	165:170	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	33	from	GL1	247:249	arg1	fractions					154:162	two purified fractions	141:162	two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1	141:249	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	6	34	theme	short-chain	744:754	arg1	SCFA					768:771	SCFA	768:771	SCFA	768:771	Additionally, the total short-chain fatty acid (SCFA) production significantly increased, especially the major SCFA of formic, lactic, and acetic acid.					
37170624	6	34	theme	short-chain	744:754	arg1	acid					762:765	short-chain fatty acid	744:765	the total short-chain fatty acid (SCFA) production	734:783	Additionally, the total short-chain fatty acid (SCFA) production significantly increased, especially the major SCFA of formic, lactic, and acetic acid.					
37170624	1	35	theme	cultures	323:330	arg1	fermentation					286:297	in vitro fermentation	277:297	in vitro fermentation of pure and human fecal cultures	277:330	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	36	dep	in	277:278	arg1	vitro					280:284	vitro	280:284	vitro	280:284	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	6	37	theme	total	738:742	arg1	production					774:783	the total short-chain fatty acid (SCFA) production	734:783	the total short-chain fatty acid (SCFA) production	734:783	Additionally, the total short-chain fatty acid (SCFA) production significantly increased, especially the major SCFA of formic, lactic, and acetic acid.					
37170624	8	38	contain	have	1188:1191	arg2	potential					1197:1205	the potential to be developed as a prebiotic	1193:1236	the potential to be developed as a prebiotic	1193:1236	These results suggest that GL1-E1 and GL1-E2 have the potential to be developed as a prebiotic.					
37170624	8	38	contain	have	1188:1191	arg1	GL1-E2					1181:1186	GL1-E2	1181:1186	GL1-E2	1181:1186	These results suggest that GL1-E1 and GL1-E2 have the potential to be developed as a prebiotic.					
37170624	8	38	contain	have	1188:1191	arg1	GL1-E1					1170:1175	GL1-E1	1170:1175	GL1-E1	1170:1175	These results suggest that GL1-E1 and GL1-E2 have the potential to be developed as a prebiotic.					
37170624	6	39	theme	major	825:829	arg1	SCFA					831:834	the major SCFA	821:834	the major SCFA of formic, lactic, and acetic acid	821:869	Additionally, the total short-chain fatty acid (SCFA) production significantly increased, especially the major SCFA of formic, lactic, and acetic acid.					
37170624	1	40	theme	Lacticaseibacillus	218:235	arg1	GL1					247:249	Lacticaseibacillus paracasei GL1	218:249	Lacticaseibacillus paracasei GL1	218:249	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	4	41	used	utilized	631:638	arg2	both					598:601	both	598:601	both	598:601	It was also found that both were gradually degraded and utilized by the gut microbiota.					
37170624	1	42	theme	purified	145:152	arg1	GL1-E2					176:181	GL1-E2	176:181	GL1-E2	176:181	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	42	theme	purified	145:152	arg1	GL1-E1					165:170	GL1-E1	165:170	GL1-E1	165:170	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	1	42	theme	purified	145:152	arg1	fractions					154:162	two purified fractions	141:162	two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1	141:249	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	0	43	from	GL1	108:110	arg1	digestion					19:27	In vitro simulated digestion	0:27	In vitro simulated digestion	0:27	In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1.					
37170624	0	43	from	GL1	108:110	arg1	fermentation					39:50	fecal fermentation	33:50	fecal fermentation	33:50	In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1.					
37170624	3	44	used	used	514:517	arg2	GL1-E2					500:505	GL1-E2	500:505	GL1-E2	500:505	Additionally, GL1-E1 and GL1-E2 can be used as substrates for Lactobacillus and Lactococcus growth.					
37170624	3	44	used	used	514:517	arg2	substrates					522:531	substrates	522:531	substrates for Lactobacillus and Lactococcus growth	522:572	Additionally, GL1-E1 and GL1-E2 can be used as substrates for Lactobacillus and Lactococcus growth.					
37170624	3	44	used	used	514:517	arg2	GL1-E1					489:494	GL1-E1	489:494	GL1-E1	489:494	Additionally, GL1-E1 and GL1-E2 can be used as substrates for Lactobacillus and Lactococcus growth.					
37170624	1	45	theme	fractions	154:162	arg1	properties					127:136	The prebiotic properties	113:136	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1	113:249	The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures.					
37170624	0	46	theme	exopolysaccharides	55:72	arg1	digestion					19:27	In vitro simulated digestion	0:27	In vitro simulated digestion	0:27	In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1.					
37170624	0	46	theme	exopolysaccharides	55:72	arg1	fermentation					39:50	fecal fermentation	33:50	fecal fermentation	33:50	In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1.					
37170624	0	47	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1.					
37031863	7	0	theme	chemical	639:646	arg1	compatibility					666:678	both chemical and physiological compatibility	634:678	both chemical and physiological compatibility	634:678	It showed both chemical and physiological compatibility.					
37031863	3	1	dep	in	354:355	arg1	situ					357:360	situ	357:360	situ	357:360	To achieve this and address the issues of ocular retention and controlled release an in situ gel of metformin was developed.					
37031863	1	2	theme	anti-diabetic	134:146	arg1	action					148:153	its anti-diabetic action	130:153	its anti-diabetic action	130:153	Metformin is known to lower inflammation, independent of its anti-diabetic action.					
37031863	11	3	theme	ex	910:911	arg1	study					929:933	an ex vivo permeation study	907:933	an ex vivo permeation study	907:933	Supported by an ex vivo permeation study, it showed potential for prolonged action.					
37031863	9	4	theme	zero-order	794:803	arg1	kinetics					805:812	zero-order kinetics	794:812	zero-order kinetics	794:812	MF5 exhibited sustained release of metformin for 8h that fitted best with zero-order kinetics.					
37031863	1	5	theme	action	148:153	arg1	independent					115:125	independent	115:125	independent	115:125	Metformin is known to lower inflammation, independent of its anti-diabetic action.					
37031863	6	6	theme	optimized	601:609	arg1	formulation					611:621	the optimized formulation	597:621	the optimized formulation	597:621	MF5 was selected as the optimized formulation.					
37031863	6	6	theme	optimized	601:609	arg1	MF5					577:579	MF5	577:579	MF5	577:579	MF5 was selected as the optimized formulation.					
37031863	4	7	theme	sodium	431:436	arg1	hyaluronate					438:448	sodium hyaluronate	431:448	sodium hyaluronate	431:448	The formulations were prepared using sodium hyaluronate, hypromellose, and gellan gum.					
37031863	3	8	theme	release	343:349	arg1	issues					301:306	the issues	297:306	the issues of ocular retention and controlled release	297:349	To achieve this and address the issues of ocular retention and controlled release an in situ gel of metformin was developed.					
37031863	13	9	theme	translational	1095:1107	arg1	alternative					1129:1139	a safe alternative	1122:1139	a safe alternative to steroids for managing ocular inflammation	1122:1184	MF5 shows translational potential as a safe alternative to steroids for managing ocular inflammation.					
37031863	13	9	theme	translational	1095:1107	arg1	potential					1109:1117	translational potential	1095:1117	translational potential	1095:1117	MF5 shows translational potential as a safe alternative to steroids for managing ocular inflammation.					
37031863	9	10	theme	sustained	734:742	arg1	release					744:750	sustained release	734:750	sustained release of metformin for 8h that fitted best with zero-order kinetics	734:812	MF5 exhibited sustained release of metformin for 8h that fitted best with zero-order kinetics.					
37031863	13	11	theme	ocular	1166:1171	arg1	inflammation					1173:1184	ocular inflammation	1166:1184	ocular inflammation	1166:1184	MF5 shows translational potential as a safe alternative to steroids for managing ocular inflammation.					
37031863	12	12	theme	standard	1070:1077	arg1	drug					1079:1082	the standard drug	1066:1082	the standard drug	1066:1082	It showed a significant reduction in ocular inflammation that was comparable to that of the standard drug.					
37031863	3	13	theme	controlled	332:341	arg1	release					343:349	controlled release	332:349	controlled release	332:349	To achieve this and address the issues of ocular retention and controlled release an in situ gel of metformin was developed.					
37031863	4	14	theme	gellan	469:474	arg1	gum					476:478	gellan gum	469:478	gellan gum	469:478	The formulations were prepared using sodium hyaluronate, hypromellose, and gellan gum.					
37031863	12	15	theme	ocular	1015:1020	arg1	inflammation					1022:1033	ocular inflammation	1015:1033	ocular inflammation	1015:1033	It showed a significant reduction in ocular inflammation that was comparable to that of the standard drug.					
37031863	1	16	dep	inflammation	101:112	arg1	lower					95:99	lower	95:99	lower	95:99	Metformin is known to lower inflammation, independent of its anti-diabetic action.					
37031863	3	17	dep	developed	383:391	arg1	achieve					272:278	achieve	272:278	achieve this	272:283	To achieve this and address the issues of ocular retention and controlled release an in situ gel of metformin was developed.					
37031863	3	17	dep	developed	383:391	arg1	address					289:295	address	289:295	address the issues of ocular retention and controlled release	289:349	To achieve this and address the issues of ocular retention and controlled release an in situ gel of metformin was developed.					
37031863	0	18	theme	Ocular	0:5	arg1	Delivery					7:14	Ocular Delivery	0:14	Ocular Delivery of Metformin for Sustained Release and In Vivo Efficacy.	0:71	Ocular Delivery of Metformin for Sustained Release and In Vivo Efficacy.					
37031863	3	19	theme	in	354:355	arg1	gel					362:364	an in situ gel	351:364	an in situ gel of metformin	351:377	To achieve this and address the issues of ocular retention and controlled release an in situ gel of metformin was developed.					
37031863	12	20	from	reduction	1002:1010	arg1	inflammation					1022:1033	ocular inflammation	1015:1033	ocular inflammation	1015:1033	It showed a significant reduction in ocular inflammation that was comparable to that of the standard drug.					
37031863	12	21	theme	significant	990:1000	arg1	reduction					1002:1010	a significant reduction	988:1010	a significant reduction in ocular inflammation that was comparable to that of the standard drug	988:1082	It showed a significant reduction in ocular inflammation that was comparable to that of the standard drug.					
37031863	12	21	theme	significant	990:1000	arg1	comparable					1044:1053	comparable	1044:1053	comparable	1044:1053	It showed a significant reduction in ocular inflammation that was comparable to that of the standard drug.					
37031863	10	22	theme	release	828:834	arg1	mode					836:839	the release mode	824:839	the release mode	824:839	Further, the release mode was found to be close to the Korsmeyer-Peppas model.					
37031863	10	22	theme	release	828:834	arg1	model					887:891	the Korsmeyer-Peppas model	866:891	the Korsmeyer-Peppas model	866:891	Further, the release mode was found to be close to the Korsmeyer-Peppas model.					
37031863	11	23	theme	permeation	918:927	arg1	study					929:933	an ex vivo permeation study	907:933	an ex vivo permeation study	907:933	Supported by an ex vivo permeation study, it showed potential for prolonged action.					
37031863	2	24	theme	topical	162:168	arg1	strategy					201:208	a therapeutic strategy	187:208	a therapeutic strategy for managing ocular inflammation associated with diabetes	187:266	Thus, topical metformin can be a therapeutic strategy for managing ocular inflammation associated with diabetes.					
37031863	2	24	theme	topical	162:168	arg1	metformin					170:178	topical metformin	162:178	topical metformin	162:178	Thus, topical metformin can be a therapeutic strategy for managing ocular inflammation associated with diabetes.					
37031863	0	25	dep	In	55:56	arg1	Vivo					58:61	Vivo	58:61	Vivo	58:61	Ocular Delivery of Metformin for Sustained Release and In Vivo Efficacy.					
37031863	0	26	theme	Sustained	33:41	arg1	Release					43:49	Sustained Release	33:49	Sustained Release	33:49	Ocular Delivery of Metformin for Sustained Release and In Vivo Efficacy.					
37031863	0	27	theme	Metformin	19:27	arg1	Delivery					7:14	Ocular Delivery	0:14	Ocular Delivery of Metformin for Sustained Release and In Vivo Efficacy.	0:71	Ocular Delivery of Metformin for Sustained Release and In Vivo Efficacy.					
37031863	5	28	theme	gelling	525:531	arg1	time/capacity					533:545	gelling time/capacity	525:545	gelling time/capacity	525:545	The composition was optimized by monitoring gelling time/capacity, viscosity, and mucoadhesion.					
37031863	3	29	theme	metformin	369:377	arg1	gel					362:364	an in situ gel	351:364	an in situ gel of metformin	351:377	To achieve this and address the issues of ocular retention and controlled release an in situ gel of metformin was developed.					
37031863	11	30	theme	prolonged	960:968	arg1	action					970:975	prolonged action	960:975	prolonged action	960:975	Supported by an ex vivo permeation study, it showed potential for prolonged action.					
37031863	0	31	theme	In	55:56	arg1	Efficacy					63:70	In Vivo Efficacy	55:70	In Vivo Efficacy	55:70	Ocular Delivery of Metformin for Sustained Release and In Vivo Efficacy.					
37031863	9	32	theme	metformin	755:763	arg1	release					744:750	sustained release	734:750	sustained release of metformin for 8h that fitted best with zero-order kinetics	734:812	MF5 exhibited sustained release of metformin for 8h that fitted best with zero-order kinetics.					
37031863	2	33	theme	therapeutic	189:199	arg1	strategy					201:208	a therapeutic strategy	187:208	a therapeutic strategy for managing ocular inflammation associated with diabetes	187:266	Thus, topical metformin can be a therapeutic strategy for managing ocular inflammation associated with diabetes.					
37031863	2	33	theme	therapeutic	189:199	arg1	metformin					170:178	topical metformin	162:178	topical metformin	162:178	Thus, topical metformin can be a therapeutic strategy for managing ocular inflammation associated with diabetes.					
37031863	10	34	theme	Korsmeyer-Peppas	870:885	arg1	mode					836:839	the release mode	824:839	the release mode	824:839	Further, the release mode was found to be close to the Korsmeyer-Peppas model.					
37031863	10	34	theme	Korsmeyer-Peppas	870:885	arg1	model					887:891	the Korsmeyer-Peppas model	866:891	the Korsmeyer-Peppas model	866:891	Further, the release mode was found to be close to the Korsmeyer-Peppas model.					
37031863	11	35	dep	ex	910:911	arg1	vivo					913:916	vivo	913:916	vivo	913:916	Supported by an ex vivo permeation study, it showed potential for prolonged action.					
37031863	1	36	theme	independent	115:125	arg1	inflammation					101:112	inflammation	101:112	inflammation	101:112	Metformin is known to lower inflammation, independent of its anti-diabetic action.					
37031863	3	37	theme	ocular	311:316	arg1	retention					318:326	ocular retention	311:326	ocular retention	311:326	To achieve this and address the issues of ocular retention and controlled release an in situ gel of metformin was developed.					
37031863	7	38	theme	physiological	652:664	arg1	compatibility					666:678	both chemical and physiological compatibility	634:678	both chemical and physiological compatibility	634:678	It showed both chemical and physiological compatibility.					
37031863	3	39	theme	retention	318:326	arg1	issues					301:306	the issues	297:306	the issues of ocular retention and controlled release	297:349	To achieve this and address the issues of ocular retention and controlled release an in situ gel of metformin was developed.					
37031863	2	40	theme	ocular	223:228	arg1	inflammation					230:241	ocular inflammation	223:241	ocular inflammation associated with diabetes	223:266	Thus, topical metformin can be a therapeutic strategy for managing ocular inflammation associated with diabetes.					
37031863	13	41	theme	safe	1124:1127	arg1	alternative					1129:1139	a safe alternative	1122:1139	a safe alternative to steroids for managing ocular inflammation	1122:1184	MF5 shows translational potential as a safe alternative to steroids for managing ocular inflammation.					
37031863	13	41	theme	safe	1124:1127	arg1	potential					1109:1117	translational potential	1095:1117	translational potential	1095:1117	MF5 shows translational potential as a safe alternative to steroids for managing ocular inflammation.					
36901883	0	0	theme	Controlled	94:103	arg1	Release					105:111	Controlled Release	94:111	Controlled Release of Plant-Derived Polyphenols	94:140	Chitosan Sponges with Instantaneous Shape Recovery and Multistrain Antibacterial Activity for Controlled Release of Plant-Derived Polyphenols.					
36901883	8	1	theme	release	1622:1628	arg1	mechanism					1630:1638	a pseudo-Fickian diffusion release mechanism	1595:1638	a pseudo-Fickian diffusion release mechanism	1595:1638	By linearly fitting the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models, a pseudo-Fickian diffusion release mechanism was predicted.					
36901883	8	2	with	sponges	1545:1551	arg1	models					1587:1592	the Korsmeyer-Peppas kinetic models	1558:1592	the Korsmeyer-Peppas kinetic models	1558:1592	By linearly fitting the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models, a pseudo-Fickian diffusion release mechanism was predicted.					
36901883	8	3	theme	CS	1542:1543	arg1	sponges					1545:1551	the CS sponges	1538:1551	the CS sponges with the Korsmeyer-Peppas kinetic models	1538:1592	By linearly fitting the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models, a pseudo-Fickian diffusion release mechanism was predicted.					
36901883	7	4	theme	preparation	1451:1461	arg1	strategy					1463:1470	the preparation strategy	1447:1470	the preparation strategy	1447:1470	It was found that CCM release was dependent on the composition and the preparation strategy of sponges.					
36901883	8	5	theme	CCM	1508:1510	arg1	data					1528:1531	the CCM kinetic release data	1504:1531	the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models	1504:1592	By linearly fitting the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models, a pseudo-Fickian diffusion release mechanism was predicted.					
36901883	5	6	dep	Gram-positive	1008:1020	arg1	monocytogenes					1067:1079	Listeria monocytogenes	1058:1079	Listeria monocytogenes (L. monocytogenes)	1058:1098	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	5	6	dep	Gram-positive	1008:1020	arg1	aureus					1038:1043	Staphylococcus aureus	1023:1043	Staphylococcus aureus (S. aureus)	1023:1055	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	7	7	theme	CCM	1398:1400	arg1	release					1402:1408	CCM release	1398:1408	CCM release	1398:1408	It was found that CCM release was dependent on the composition and the preparation strategy of sponges.					
36901883	2	8	link	plant-derived	465:477	arg1	polyphenols					479:489	plant-derived polyphenols	465:489	plant-derived polyphenols	465:489	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	4	9	theme	gelation	818:825	arg1	conditions					827:836	gelation conditions	818:836	gelation conditions (either cryogelation or room-temperature gelation)	818:887	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	4	9	theme	gelation	818:825	arg1	cryogelation					846:857	cryogelation	846:857	cryogelation	846:857	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	4	9	theme	gelation	818:825	arg1	gelation					879:886	room-temperature gelation	862:886	room-temperature gelation	862:886	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	5	10	theme	water-triggered	914:928	arg1	recovery					936:943	complete water-triggered shape recovery	905:943	complete water-triggered shape recovery	905:943	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	5	11	dep	coli	1132:1135	arg1	typhimurium					1175:1185	S. typhimurium	1172:1185	S. typhimurium	1172:1185	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	5	11	dep	coli	1132:1135	arg1	coli					1141:1144	E. coli	1138:1144	E. coli	1138:1144	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	4	12	theme	cross-linking	793:805	arg1	ratio					807:811	cross-linking ratio	793:811	cross-linking ratio	793:811	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	5	13	dep	aureus	1038:1043	arg1	aureus					1049:1054	S. aureus	1046:1054	S. aureus	1046:1054	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	5	14	theme	Listeria	1058:1065	arg1	L.					1082:1083	L.	1082:1083	L.	1082:1083	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	5	14	theme	Listeria	1058:1065	arg1	monocytogenes					1067:1079	Listeria monocytogenes	1058:1079	Listeria monocytogenes (L. monocytogenes)	1058:1098	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	5	14	theme	Listeria	1058:1065	arg1	aureus					1038:1043	Staphylococcus aureus	1023:1043	Staphylococcus aureus (S. aureus)	1023:1055	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	3	15	theme	electron	636:643	arg1	SEM					657:659	SEM	657:659	SEM	657:659	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	3	15	theme	electron	636:643	arg1	microscopy					645:654	scanning electron microscopy	627:654	scanning electron microscopy (SEM)	627:660	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	0	16	theme	Polyphenols	130:140	arg1	Release					105:111	Controlled Release	94:111	Controlled Release of Plant-Derived Polyphenols	94:140	Chitosan Sponges with Instantaneous Shape Recovery and Multistrain Antibacterial Activity for Controlled Release of Plant-Derived Polyphenols.					
36901883	1	17	with	materials	159:167	arg1	features					183:190	multiple features	174:190	multiple features	174:190	Biomass-derived materials with multiple features are seldom reported so far.					
36901883	5	18	theme	complete	905:912	arg1	recovery					936:943	complete water-triggered shape recovery	905:943	complete water-triggered shape recovery	905:943	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	2	19	theme	healthcare	300:309	arg1	applications					311:322	point-of-use healthcare applications	287:322	point-of-use healthcare applications	287:322	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	4	20	dep	cryogelation	846:857	arg1	either					839:844	either	839:844	either	839:844	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	4	21	theme	main	720:723	arg1	features					725:732	The main features	716:732	The main features of sponges	716:743	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	0	22	theme	Plant-Derived	116:128	arg1	Polyphenols					130:140	Plant-Derived Polyphenols	116:140	Plant-Derived Polyphenols	116:140	Chitosan Sponges with Instantaneous Shape Recovery and Multistrain Antibacterial Activity for Controlled Release of Plant-Derived Polyphenols.					
36901883	8	23	theme	Korsmeyer-Peppas	1562:1577	arg1	models					1587:1592	the Korsmeyer-Peppas kinetic models	1558:1592	the Korsmeyer-Peppas kinetic models	1558:1592	By linearly fitting the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models, a pseudo-Fickian diffusion release mechanism was predicted.					
36901883	2	24	theme	point-of-use	287:298	arg1	applications					311:322	point-of-use healthcare applications	287:322	point-of-use healthcare applications	287:322	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	8	25	theme	kinetic	1579:1585	arg1	models					1587:1592	the Korsmeyer-Peppas kinetic models	1558:1592	the Korsmeyer-Peppas kinetic models	1558:1592	By linearly fitting the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models, a pseudo-Fickian diffusion release mechanism was predicted.					
36901883	5	26	theme	Gram-negative	1105:1117	arg1	strains					1189:1195	Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains	1008:1195	strains	1189:1195	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	3	27	theme	Fourier-transform	579:595	arg1	FTIR					607:610	FTIR	607:610	FTIR	607:610	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	3	27	theme	Fourier-transform	579:595	arg1	spectroscopy					613:624	Fourier-transform infrared (FTIR) spectroscopy	579:624	Fourier-transform infrared (FTIR) spectroscopy	579:624	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	3	28	theme	scanning	627:634	arg1	SEM					657:659	SEM	657:659	SEM	657:659	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	3	28	theme	scanning	627:634	arg1	microscopy					645:654	scanning electron microscopy	627:654	scanning electron microscopy (SEM)	627:660	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	4	29	dep	conditions	827:836	arg1	conditions					827:836	gelation conditions	818:836	gelation conditions (either cryogelation or room-temperature gelation)	818:887	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	4	29	dep	conditions	827:836	arg1	cryogelation					846:857	cryogelation	846:857	cryogelation	846:857	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	4	29	dep	conditions	827:836	arg1	gelation					879:886	room-temperature gelation	862:886	room-temperature gelation	862:886	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	2	30	theme	antioxidant	414:424	arg1	properties					426:435	antioxidant properties	414:435	antioxidant properties	414:435	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	5	31	theme	scavenging	1222:1231	arg1	activity					1233:1240	good radical scavenging activity	1209:1240	good radical scavenging activity	1209:1240	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	4	32	theme	CS	775:776	arg1	concentration					778:790	the CS concentration	771:790	the CS concentration	771:790	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	2	33	theme	glutaraldehyde	341:354	arg1	cross-linking					361:373	glutaraldehyde (GA) cross-linking	341:373	glutaraldehyde (GA) cross-linking	341:373	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	7	34	theme	sponges	1475:1481	arg1	strategy					1463:1470	the preparation strategy	1447:1470	the preparation strategy	1447:1470	It was found that CCM release was dependent on the composition and the preparation strategy of sponges.					
36901883	7	34	theme	sponges	1475:1481	arg1	composition					1431:1441	composition	1431:1441	composition	1431:1441	It was found that CCM release was dependent on the composition and the preparation strategy of sponges.					
36901883	0	35	theme	Instantaneous	22:34	arg1	Recovery					42:49	Instantaneous Shape Recovery	22:49	Instantaneous Shape Recovery	22:49	Chitosan Sponges with Instantaneous Shape Recovery and Multistrain Antibacterial Activity for Controlled Release of Plant-Derived Polyphenols.					
36901883	4	36	theme	room-temperature	862:877	arg1	conditions					827:836	gelation conditions	818:836	gelation conditions (either cryogelation or room-temperature gelation)	818:887	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	4	36	theme	room-temperature	862:877	arg1	gelation					879:886	room-temperature gelation	862:886	room-temperature gelation	862:886	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	3	37	theme	morphological	510:522	arg1	properties					540:549	Their structural, morphological, and mechanical properties	492:549	Their structural, morphological, and mechanical properties	492:549	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	2	38	theme	plant-derived	465:477	arg1	polyphenols					479:489	plant-derived polyphenols	465:489	plant-derived polyphenols	465:489	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	2	39	with	sponges	246:252	arg1	functions					273:281	complementary functions	259:281	complementary functions for point-of-use healthcare applications	259:322	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	3	40	theme	infrared	597:604	arg1	FTIR					607:610	FTIR	607:610	FTIR	607:610	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	3	40	theme	infrared	597:604	arg1	spectroscopy					613:624	Fourier-transform infrared (FTIR) spectroscopy	579:624	Fourier-transform infrared (FTIR) spectroscopy	579:624	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	8	41	theme	kinetic	1512:1518	arg1	data					1528:1531	the CCM kinetic release data	1504:1531	the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models	1504:1592	By linearly fitting the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models, a pseudo-Fickian diffusion release mechanism was predicted.					
36901883	5	42	theme	remarkable	964:973	arg1	properties					989:998	remarkable antibacterial properties	964:998	remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains	964:1195	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	5	43	dep	strains	1189:1195	arg1	coli					1132:1135	Escherichia coli	1120:1135	Escherichia coli (E. coli)	1120:1145	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	5	43	dep	strains	1189:1195	arg1	typhimurium					1159:1169	Salmonella typhimurium	1148:1169	Salmonella typhimurium	1148:1169	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	3	44	theme	structural	498:507	arg1	properties					540:549	Their structural, morphological, and mechanical properties	492:549	Their structural, morphological, and mechanical properties	492:549	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	2	45	theme	controlled	442:451	arg1	delivery					453:460	controlled delivery	442:460	controlled delivery of plant-derived polyphenols	442:489	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	0	46	theme	Shape	36:40	arg1	Recovery					42:49	Instantaneous Shape Recovery	22:49	Instantaneous Shape Recovery	22:49	Chitosan Sponges with Instantaneous Shape Recovery and Multistrain Antibacterial Activity for Controlled Release of Plant-Derived Polyphenols.					
36901883	8	47	theme	diffusion	1612:1620	arg1	mechanism					1630:1638	a pseudo-Fickian diffusion release mechanism	1595:1638	a pseudo-Fickian diffusion release mechanism	1595:1638	By linearly fitting the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models, a pseudo-Fickian diffusion release mechanism was predicted.					
36901883	6	48	theme	gastrointestinal	1356:1371	arg1	media					1373:1377	simulated gastrointestinal media	1346:1377	simulated gastrointestinal media	1346:1377	The release profile of a plant-derived polyphenol, namely curcumin (CCM), was investigated at 37 °C in simulated gastrointestinal media.					
36901883	5	49	dep	L.	1082:1083	arg1	monocytogenes					1085:1097	L. monocytogenes	1082:1097	L. monocytogenes	1082:1097	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	2	50	theme	polyphenols	479:489	arg1	properties					426:435	antioxidant properties	414:435	antioxidant properties	414:435	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	2	50	theme	polyphenols	479:489	arg1	delivery					453:460	controlled delivery	442:460	controlled delivery of plant-derived polyphenols	442:489	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	2	50	theme	polyphenols	479:489	arg1	activity					404:411	antibacterial activity	390:411	antibacterial activity	390:411	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	5	51	theme	shape	930:934	arg1	recovery					936:943	complete water-triggered shape recovery	905:943	complete water-triggered shape recovery	905:943	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	0	52	theme	Multistrain	55:65	arg1	Activity					81:88	Multistrain Antibacterial Activity	55:88	Multistrain Antibacterial Activity	55:88	Chitosan Sponges with Instantaneous Shape Recovery and Multistrain Antibacterial Activity for Controlled Release of Plant-Derived Polyphenols.					
36901883	8	53	theme	pseudo-Fickian	1597:1610	arg1	mechanism					1630:1638	a pseudo-Fickian diffusion release mechanism	1595:1638	a pseudo-Fickian diffusion release mechanism	1595:1638	By linearly fitting the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models, a pseudo-Fickian diffusion release mechanism was predicted.					
36901883	6	54	theme	simulated	1346:1354	arg1	media					1373:1377	simulated gastrointestinal media	1346:1377	simulated gastrointestinal media	1346:1377	The release profile of a plant-derived polyphenol, namely curcumin (CCM), was investigated at 37 °C in simulated gastrointestinal media.					
36901883	6	55	link	plant-derived	1268:1280	arg1	polyphenol					1282:1291	a plant-derived polyphenol	1266:1291	a plant-derived polyphenol	1266:1291	The release profile of a plant-derived polyphenol, namely curcumin (CCM), was investigated at 37 °C in simulated gastrointestinal media.					
36901883	6	55	link	plant-derived	1268:1280	arg1	curcumin					1301:1308	curcumin	1301:1308	namely curcumin (CCM)	1294:1314	The release profile of a plant-derived polyphenol, namely curcumin (CCM), was investigated at 37 °C in simulated gastrointestinal media.					
36901883	6	56	theme	polyphenol	1282:1291	arg1	profile					1255:1261	The release profile	1243:1261	The release profile of a plant-derived polyphenol, namely curcumin (CCM),	1243:1315	The release profile of a plant-derived polyphenol, namely curcumin (CCM), was investigated at 37 °C in simulated gastrointestinal media.					
36901883	8	57	from	sponges	1545:1551	arg1	data					1528:1531	the CCM kinetic release data	1504:1531	the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models	1504:1592	By linearly fitting the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models, a pseudo-Fickian diffusion release mechanism was predicted.					
36901883	5	58	theme	good	1209:1212	arg1	activity					1233:1240	good radical scavenging activity	1209:1240	good radical scavenging activity	1209:1240	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	7	59	dep	composition	1431:1441	arg1	the					1427:1429	the	1427:1429	the	1427:1429	It was found that CCM release was dependent on the composition and the preparation strategy of sponges.					
36901883	2	60	theme	chitosan	232:239	arg1	sponges					246:252	new chitosan (CS) sponges	228:252	new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications	228:322	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	6	61	theme	plant-derived	1268:1280	arg1	polyphenol					1282:1291	a plant-derived polyphenol	1266:1291	a plant-derived polyphenol	1266:1291	The release profile of a plant-derived polyphenol, namely curcumin (CCM), was investigated at 37 °C in simulated gastrointestinal media.					
36901883	6	61	theme	plant-derived	1268:1280	arg1	curcumin					1301:1308	curcumin	1301:1308	namely curcumin (CCM)	1294:1314	The release profile of a plant-derived polyphenol, namely curcumin (CCM), was investigated at 37 °C in simulated gastrointestinal media.					
36901883	0	62	with	Sponges	9:15	arg1	Recovery					42:49	Instantaneous Shape Recovery	22:49	Instantaneous Shape Recovery	22:49	Chitosan Sponges with Instantaneous Shape Recovery and Multistrain Antibacterial Activity for Controlled Release of Plant-Derived Polyphenols.					
36901883	0	62	with	Sponges	9:15	arg1	Activity					81:88	Multistrain Antibacterial Activity	55:88	Multistrain Antibacterial Activity	55:88	Chitosan Sponges with Instantaneous Shape Recovery and Multistrain Antibacterial Activity for Controlled Release of Plant-Derived Polyphenols.					
36901883	1	63	theme	Biomass-derived	143:157	arg1	materials					159:167	Biomass-derived materials	143:167	Biomass-derived materials with multiple features	143:190	Biomass-derived materials with multiple features are seldom reported so far.					
36901883	5	64	theme	radical	1214:1220	arg1	activity					1233:1240	good radical scavenging activity	1209:1240	good radical scavenging activity	1209:1240	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	2	65	theme	new	228:230	arg1	CS					242:243	CS	242:243	CS	242:243	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	2	65	theme	new	228:230	arg1	chitosan					232:239	new chitosan	228:239	new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications	228:322	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	3	66	theme	uniaxial	667:674	arg1	measurements					688:699	uniaxial compression measurements	667:699	uniaxial compression measurements	667:699	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	4	67	theme	sponges	737:743	arg1	features					725:732	The main features	716:732	The main features of sponges	716:743	The main features of sponges were modulated by varying the CS concentration, cross-linking ratio, and gelation conditions (either cryogelation or room-temperature gelation).					
36901883	1	68	link	Biomass-derived	143:157	arg1	materials					159:167	Biomass-derived materials	143:167	Biomass-derived materials with multiple features	143:190	Biomass-derived materials with multiple features are seldom reported so far.					
36901883	8	69	theme	release	1520:1526	arg1	data					1528:1531	the CCM kinetic release data	1504:1531	the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models	1504:1592	By linearly fitting the CCM kinetic release data from the CS sponges with the Korsmeyer-Peppas kinetic models, a pseudo-Fickian diffusion release mechanism was predicted.					
36901883	3	70	theme	compression	676:686	arg1	measurements					688:699	uniaxial compression measurements	667:699	uniaxial compression measurements	667:699	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	2	71	theme	GA	357:358	arg1	cross-linking					361:373	glutaraldehyde (GA) cross-linking	341:373	glutaraldehyde (GA) cross-linking	341:373	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	5	72	theme	antibacterial	975:987	arg1	properties					989:998	remarkable antibacterial properties	964:998	remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains	964:1195	They exhibited complete water-triggered shape recovery after compression, remarkable antibacterial properties against Gram-positive (Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes)) and Gram-negative (Escherichia coli (E. coli), Salmonella typhimurium (S. typhimurium)) strains, as well as good radical scavenging activity.					
36901883	2	73	theme	complementary	259:271	arg1	functions					273:281	complementary functions	259:281	complementary functions for point-of-use healthcare applications	259:322	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36901883	1	74	theme	multiple	174:181	arg1	features					183:190	multiple features	174:190	multiple features	174:190	Biomass-derived materials with multiple features are seldom reported so far.					
36901883	3	75	theme	mechanical	529:538	arg1	properties					540:549	Their structural, morphological, and mechanical properties	492:549	Their structural, morphological, and mechanical properties	492:549	Their structural, morphological, and mechanical properties were thoroughly assessed by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and uniaxial compression measurements, respectively.					
36901883	0	76	theme	Antibacterial	67:79	arg1	Activity					81:88	Multistrain Antibacterial Activity	55:88	Multistrain Antibacterial Activity	55:88	Chitosan Sponges with Instantaneous Shape Recovery and Multistrain Antibacterial Activity for Controlled Release of Plant-Derived Polyphenols.					
36901883	6	77	theme	release	1247:1253	arg1	profile					1255:1261	The release profile	1243:1261	The release profile of a plant-derived polyphenol, namely curcumin (CCM),	1243:1315	The release profile of a plant-derived polyphenol, namely curcumin (CCM), was investigated at 37 °C in simulated gastrointestinal media.					
36901883	2	78	theme	antibacterial	390:402	arg1	activity					404:411	antibacterial activity	390:411	antibacterial activity	390:411	Herein, new chitosan (CS) sponges with complementary functions for point-of-use healthcare applications were prepared by glutaraldehyde (GA) cross-linking and tested for antibacterial activity, antioxidant properties, and controlled delivery of plant-derived polyphenols.					
36564693	7	0	theme	weight	1429:1434	arg1	loss					1436:1439	relatively low weight loss	1414:1439	relatively low weight loss	1414:1439	The sonicated scoured and bleached fabric's whiteness index was found to be acceptable, with relatively low weight loss; however, the bursting strength was found to be increased.					
36564693	10	1	theme	chemical	1888:1895	arg1	composition					1897:1907	the chemical composition	1884:1907	the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication	1884:2004	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	6	2	theme	dyed	1220:1223	arg1	fabric					1225:1230	reactive dyed fabric	1211:1230	reactive dyed fabric for ultrasonic pretreated fabric	1211:1263	The color matching of reactive dyed fabric for ultrasonic pretreated fabric with and without reusing bath chemicals was determined.					
36564693	3	3	theme	waste	506:510	arg1	waste					506:510	waste	506:510	waste	506:510	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	3	3	theme	waste	506:510	arg1	lot					499:501	a lot	497:501	a lot	497:501	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	2	4	theme	technologies	248:259	arg1	quest					235:239	The quest	231:239	The quest of new technologies for efficient water and energy use in cotton knit dyeing	231:316	The quest of new technologies for efficient water and energy use in cotton knit dyeing could result in significant water savings and improve environmental sustainability.					
36564693	8	5	theme	pretreated	1653:1662	arg1	fabric					1697:1702	pretreated and reuse-1 pretreated dyed knit fabric	1653:1702	pretreated and reuse-1 pretreated dyed knit fabric	1653:1702	Color fastness to light, washing, perspiration, and rubbing were found to be comparable to the conventional technique for low temperature ultrasonicated pretreated and reuse-1 pretreated dyed knit fabric.					
36564693	7	6	theme	whiteness	1365:1373	arg1	index					1375:1379	The sonicated scoured and bleached fabric's whiteness index	1321:1379	The sonicated scoured and bleached fabric's whiteness index	1321:1379	The sonicated scoured and bleached fabric's whiteness index was found to be acceptable, with relatively low weight loss; however, the bursting strength was found to be increased.					
36564693	3	7	theme	chemical	528:535	arg1	consumption					537:547	chemical consumption	528:547	chemical consumption	528:547	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	8	8	theme	reuse-1	1668:1674	arg1	fabric					1697:1702	pretreated and reuse-1 pretreated dyed knit fabric	1653:1702	pretreated and reuse-1 pretreated dyed knit fabric	1653:1702	Color fastness to light, washing, perspiration, and rubbing were found to be comparable to the conventional technique for low temperature ultrasonicated pretreated and reuse-1 pretreated dyed knit fabric.					
36564693	6	9	theme	bath	1290:1293	arg1	chemicals					1295:1303	bath chemicals	1290:1303	bath chemicals	1290:1303	The color matching of reactive dyed fabric for ultrasonic pretreated fabric with and without reusing bath chemicals was determined.					
36564693	8	10	theme	dyed	1687:1690	arg1	fabric					1697:1702	pretreated and reuse-1 pretreated dyed knit fabric	1653:1702	pretreated and reuse-1 pretreated dyed knit fabric	1653:1702	Color fastness to light, washing, perspiration, and rubbing were found to be comparable to the conventional technique for low temperature ultrasonicated pretreated and reuse-1 pretreated dyed knit fabric.					
36564693	3	11	theme	management	562:571	arg1	costs					573:577	effluent management costs	553:577	effluent management costs	553:577	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	7	12	theme	sonicated	1325:1333	arg1	fabric					1356:1361	The sonicated scoured and bleached fabric's	1321:1363	The sonicated scoured and bleached fabric's whiteness index	1321:1379	The sonicated scoured and bleached fabric's whiteness index was found to be acceptable, with relatively low weight loss; however, the bursting strength was found to be increased.					
36564693	10	13	theme	fabric	1929:1934	arg1	shape					1936:1940	the fabric shape	1925:1940	the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication	1925:2004	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	5	14	theme	color	995:999	arg1	fastness					1001:1008	color fastness	995:1008	color fastness to light	995:1017	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	4	15	from	processing	791:800	arg1	industry					817:824	the textile industry	805:824	the textile industry	805:824	The cotton knit fabric used in this study was pretreated and dyed utilizing ultrasonication at a lower temperature than conventional pretreatment and dyeing techniques in an attempt to establish ecofriendly wet processing in the textile industry.					
36564693	10	16	theme	cotton	1965:1970	arg1	fabric					1977:1982	pretreated and dyed cotton knit fabric	1945:1982	pretreated and dyed cotton knit fabric after ultrasonication	1945:2004	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	6	17	theme	pretreated	1247:1256	arg1	fabric					1258:1263	ultrasonic pretreated fabric	1236:1263	ultrasonic pretreated fabric	1236:1263	The color matching of reactive dyed fabric for ultrasonic pretreated fabric with and without reusing bath chemicals was determined.					
36564693	5	18	theme	conventional	891:902	arg1	techniques					904:913	conventional techniques	891:913	conventional techniques	891:913	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	0	19	theme	reusing	66:72	arg1	chemicals					79:87	ultrasonication and reusing bath chemicals	46:87	ultrasonication and reusing bath chemicals	46:87	Ecofriendly pretreatment of cotton fabrics by ultrasonication and reusing bath chemicals.					
36564693	10	20	theme	fabric	1977:1982	arg1	cellulose					1912:1920	cellulose	1912:1920	cellulose	1912:1920	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	10	20	theme	fabric	1977:1982	arg1	shape					1936:1940	the fabric shape	1925:1940	the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication	1925:2004	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	2	21	theme	efficient	265:273	arg1	water					275:279	efficient water	265:279	efficient water	265:279	The quest of new technologies for efficient water and energy use in cotton knit dyeing could result in significant water savings and improve environmental sustainability.					
36564693	2	22	theme	environmental	372:384	arg1	sustainability					386:399	environmental sustainability	372:399	environmental sustainability	372:399	The quest of new technologies for efficient water and energy use in cotton knit dyeing could result in significant water savings and improve environmental sustainability.					
36564693	5	23	theme	whiteness	946:954	arg1	index					956:960	whiteness index	946:960	whiteness index	946:960	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	4	24	theme	conventional	700:711	arg1	pretreatment					713:724	conventional pretreatment and dyeing techniques	700:746	pretreatment	713:724	The cotton knit fabric used in this study was pretreated and dyed utilizing ultrasonication at a lower temperature than conventional pretreatment and dyeing techniques in an attempt to establish ecofriendly wet processing in the textile industry.					
36564693	0	25	theme	Ecofriendly	0:10	arg1	pretreatment					12:23	Ecofriendly pretreatment	0:23	Ecofriendly pretreatment of cotton fabrics by ultrasonication and reusing bath chemicals.	0:88	Ecofriendly pretreatment of cotton fabrics by ultrasonication and reusing bath chemicals.					
36564693	1	26	theme	textile	94:100	arg1	industries					102:111	The textile industries	90:111	The textile industries of Bangladesh	90:125	The textile industries of Bangladesh contribute significantly to the country's economy, accounting for more than 40% of total annual export.					
36564693	2	27	from	quest	235:239	arg1	dyeing					311:316	cotton knit dyeing	299:316	cotton knit dyeing	299:316	The quest of new technologies for efficient water and energy use in cotton knit dyeing could result in significant water savings and improve environmental sustainability.					
36564693	5	28	theme	fabric	920:925	arg1	loss					970:973	weight loss	963:973	weight loss	963:973	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	5	28	theme	fabric	920:925	arg1	fastness					1001:1008	color fastness	995:1008	color fastness to light	995:1017	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	5	28	theme	fabric	920:925	arg1	properties					927:936	fabric properties	920:936	fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability	920:1106	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	5	28	theme	fabric	920:925	arg1	rubbing					1043:1049	rubbing	1043:1049	rubbing	1043:1049	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	5	28	theme	fabric	920:925	arg1	strength					985:992	bursting strength	976:992	bursting strength	976:992	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	5	28	theme	fabric	920:925	arg1	stability					1098:1106	dimensional stability	1086:1106	dimensional stability	1086:1106	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	5	28	theme	fabric	920:925	arg1	washing					1020:1026	washing	1020:1026	washing	1020:1026	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	5	28	theme	fabric	920:925	arg1	index					956:960	whiteness index	946:960	whiteness index	946:960	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	5	28	theme	fabric	920:925	arg1	strength					1058:1065	color strength	1052:1065	color strength	1052:1065	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	5	28	theme	fabric	920:925	arg1	perspiration					1029:1040	perspiration	1029:1040	perspiration	1029:1040	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	3	29	theme	Textile	402:408	arg1	processing					414:423	Textile wet processing	402:423	Textile wet processing	402:423	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	0	30	theme	fabrics	35:41	arg1	pretreatment					12:23	Ecofriendly pretreatment	0:23	Ecofriendly pretreatment of cotton fabrics by ultrasonication and reusing bath chemicals.	0:88	Ecofriendly pretreatment of cotton fabrics by ultrasonication and reusing bath chemicals.					
36564693	5	31	theme	bursting	976:983	arg1	strength					985:992	bursting strength	976:992	bursting strength	976:992	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	10	32	theme	FT-IR	1791:1795	arg1	spectroscopy					1797:1808	FT-IR spectroscopy	1791:1808	FT-IR spectroscopy	1791:1808	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	0	33	theme	ultrasonication	46:60	arg1	chemicals					79:87	ultrasonication and reusing bath chemicals	46:87	ultrasonication and reusing bath chemicals	46:87	Ecofriendly pretreatment of cotton fabrics by ultrasonication and reusing bath chemicals.					
36564693	2	34	theme	knit	306:309	arg1	dyeing					311:316	cotton knit dyeing	299:316	cotton knit dyeing	299:316	The quest of new technologies for efficient water and energy use in cotton knit dyeing could result in significant water savings and improve environmental sustainability.					
36564693	5	35	theme	conventional	1164:1175	arg1	techniques					1177:1186	conventional techniques	1164:1186	conventional techniques	1164:1186	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	3	36	dep	utilities	443:451	arg1	utilities					443:451	utilities	443:451	utilities (water and energy)	443:470	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	3	36	dep	utilities	443:451	arg1	energy					464:469	energy	464:469	energy	464:469	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	3	36	dep	utilities	443:451	arg1	water					454:458	water	454:458	water	454:458	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	10	37	theme	electron	1823:1830	arg1	microscopy					1832:1841	scanning electron microscopy	1814:1841	scanning electron microscopy (SEM)	1814:1847	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	10	37	theme	electron	1823:1830	arg1	SEM					1844:1846	SEM	1844:1846	SEM	1844:1846	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	2	38	theme	water	346:350	arg1	savings					352:358	significant water savings	334:358	significant water savings	334:358	The quest of new technologies for efficient water and energy use in cotton knit dyeing could result in significant water savings and improve environmental sustainability.					
36564693	1	39	theme	export	223:228	arg1	export					223:228	total annual export	210:228	total annual export	210:228	The textile industries of Bangladesh contribute significantly to the country's economy, accounting for more than 40% of total annual export.					
36564693	1	39	theme	export	223:228	arg1	%					205:205	more than 40%	193:205	more than 40% of total annual export	193:228	The textile industries of Bangladesh contribute significantly to the country's economy, accounting for more than 40% of total annual export.					
36564693	6	40	dep	color	1193:1197	arg1	with					1265:1268	with	1265:1268	with	1265:1268	The color matching of reactive dyed fabric for ultrasonic pretreated fabric with and without reusing bath chemicals was determined.					
36564693	6	40	dep	color	1193:1197	arg1	reusing					1282:1288	reusing	1282:1288	reusing bath chemicals	1282:1303	The color matching of reactive dyed fabric for ultrasonic pretreated fabric with and without reusing bath chemicals was determined.					
36564693	6	41	theme	ultrasonic	1236:1245	arg1	fabric					1258:1263	ultrasonic pretreated fabric	1236:1263	ultrasonic pretreated fabric	1236:1263	The color matching of reactive dyed fabric for ultrasonic pretreated fabric with and without reusing bath chemicals was determined.					
36564693	7	42	theme	low	1425:1427	arg1	loss					1436:1439	relatively low weight loss	1414:1439	relatively low weight loss	1414:1439	The sonicated scoured and bleached fabric's whiteness index was found to be acceptable, with relatively low weight loss; however, the bursting strength was found to be increased.					
36564693	6	43	theme	fabric	1225:1230	arg1	color					1193:1197	The color matching	1189:1206	The color matching of reactive dyed fabric for ultrasonic pretreated fabric with and without reusing bath chemicals	1189:1303	The color matching of reactive dyed fabric for ultrasonic pretreated fabric with and without reusing bath chemicals was determined.					
36564693	6	44	theme	reactive	1211:1218	arg1	fabric					1225:1230	reactive dyed fabric	1211:1230	reactive dyed fabric for ultrasonic pretreated fabric	1211:1263	The color matching of reactive dyed fabric for ultrasonic pretreated fabric with and without reusing bath chemicals was determined.					
36564693	8	45	theme	low	1622:1624	arg1	temperature					1626:1636	low temperature	1622:1636	low temperature ultrasonicated pretreated and reuse-1 pretreated dyed knit fabric	1622:1702	Color fastness to light, washing, perspiration, and rubbing were found to be comparable to the conventional technique for low temperature ultrasonicated pretreated and reuse-1 pretreated dyed knit fabric.					
36564693	5	46	theme	bath	831:834	arg1	chemicals					836:844	The bath chemicals	827:844	The bath chemicals	827:844	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	4	47	theme	textile	809:815	arg1	industry					817:824	the textile industry	805:824	the textile industry	805:824	The cotton knit fabric used in this study was pretreated and dyed utilizing ultrasonication at a lower temperature than conventional pretreatment and dyeing techniques in an attempt to establish ecofriendly wet processing in the textile industry.					
36564693	6	48	theme	matching	1199:1206	arg1	color					1193:1197	The color matching	1189:1206	The color matching of reactive dyed fabric for ultrasonic pretreated fabric with and without reusing bath chemicals	1189:1303	The color matching of reactive dyed fabric for ultrasonic pretreated fabric with and without reusing bath chemicals was determined.					
36564693	10	49	theme	cellulose	1912:1920	arg1	composition					1897:1907	the chemical composition	1884:1907	the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication	1884:2004	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	2	50	theme	energy	285:290	arg1	use					292:294	energy use	285:294	energy use	285:294	The quest of new technologies for efficient water and energy use in cotton knit dyeing could result in significant water savings and improve environmental sustainability.					
36564693	4	51	theme	knit	591:594	arg1	fabric					596:601	The cotton knit fabric	580:601	The cotton knit fabric used in this study	580:620	The cotton knit fabric used in this study was pretreated and dyed utilizing ultrasonication at a lower temperature than conventional pretreatment and dyeing techniques in an attempt to establish ecofriendly wet processing in the textile industry.					
36564693	3	52	theme	effluent	553:560	arg1	costs					573:577	effluent management costs	553:577	effluent management costs	553:577	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	8	53	theme	pretreated	1676:1685	arg1	fabric					1697:1702	pretreated and reuse-1 pretreated dyed knit fabric	1653:1702	pretreated and reuse-1 pretreated dyed knit fabric	1653:1702	Color fastness to light, washing, perspiration, and rubbing were found to be comparable to the conventional technique for low temperature ultrasonicated pretreated and reuse-1 pretreated dyed knit fabric.					
36564693	4	54	from	temperature	683:693	arg1	attempt					754:760	an attempt to establish ecofriendly wet processing in the textile industry	751:824	an attempt to establish ecofriendly wet processing in the textile industry	751:824	The cotton knit fabric used in this study was pretreated and dyed utilizing ultrasonication at a lower temperature than conventional pretreatment and dyeing techniques in an attempt to establish ecofriendly wet processing in the textile industry.					
36564693	7	55	theme	bleached	1347:1354	arg1	fabric					1356:1361	The sonicated scoured and bleached fabric's	1321:1363	The sonicated scoured and bleached fabric's whiteness index	1321:1379	The sonicated scoured and bleached fabric's whiteness index was found to be acceptable, with relatively low weight loss; however, the bursting strength was found to be increased.					
36564693	10	56	theme	shape	1936:1940	arg1	composition					1897:1907	the chemical composition	1884:1907	the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication	1884:2004	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	8	57	theme	knit	1692:1695	arg1	fabric					1697:1702	pretreated and reuse-1 pretreated dyed knit fabric	1653:1702	pretreated and reuse-1 pretreated dyed knit fabric	1653:1702	Color fastness to light, washing, perspiration, and rubbing were found to be comparable to the conventional technique for low temperature ultrasonicated pretreated and reuse-1 pretreated dyed knit fabric.					
36564693	1	58	theme	Bangladesh	116:125	arg1	industries					102:111	The textile industries	90:111	The textile industries of Bangladesh	90:125	The textile industries of Bangladesh contribute significantly to the country's economy, accounting for more than 40% of total annual export.					
36564693	7	59	theme	scoured	1335:1341	arg1	fabric					1356:1361	The sonicated scoured and bleached fabric's	1321:1363	The sonicated scoured and bleached fabric's whiteness index	1321:1379	The sonicated scoured and bleached fabric's whiteness index was found to be acceptable, with relatively low weight loss; however, the bursting strength was found to be increased.					
36564693	2	60	theme	new	244:246	arg1	technologies					248:259	new technologies	244:259	new technologies for efficient water and energy use	244:294	The quest of new technologies for efficient water and energy use in cotton knit dyeing could result in significant water savings and improve environmental sustainability.					
36564693	4	61	theme	lower	677:681	arg1	temperature					683:693	a lower temperature	675:693	a lower temperature than conventional pretreatment and dyeing techniques in an attempt to establish ecofriendly wet processing in the textile industry	675:824	The cotton knit fabric used in this study was pretreated and dyed utilizing ultrasonication at a lower temperature than conventional pretreatment and dyeing techniques in an attempt to establish ecofriendly wet processing in the textile industry.					
36564693	10	62	theme	dyed	1960:1963	arg1	fabric					1977:1982	pretreated and dyed cotton knit fabric	1945:1982	pretreated and dyed cotton knit fabric after ultrasonication	1945:2004	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	0	63	theme	bath	74:77	arg1	chemicals					79:87	ultrasonication and reusing bath chemicals	46:87	ultrasonication and reusing bath chemicals	46:87	Ecofriendly pretreatment of cotton fabrics by ultrasonication and reusing bath chemicals.					
36564693	1	64	theme	total	210:214	arg1	export					223:228	total annual export	210:228	total annual export	210:228	The textile industries of Bangladesh contribute significantly to the country's economy, accounting for more than 40% of total annual export.					
36564693	10	65	theme	pretreated	1945:1954	arg1	fabric					1977:1982	pretreated and dyed cotton knit fabric	1945:1982	pretreated and dyed cotton knit fabric after ultrasonication	1945:2004	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	4	66	theme	dyeing	730:735	arg1	techniques					737:746	conventional pretreatment and dyeing techniques	700:746	techniques	737:746	The cotton knit fabric used in this study was pretreated and dyed utilizing ultrasonication at a lower temperature than conventional pretreatment and dyeing techniques in an attempt to establish ecofriendly wet processing in the textile industry.					
36564693	10	67	theme	knit	1972:1975	arg1	fabric					1977:1982	pretreated and dyed cotton knit fabric	1945:1982	pretreated and dyed cotton knit fabric after ultrasonication	1945:2004	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	5	68	theme	color	1052:1056	arg1	strength					1058:1065	color strength	1052:1065	color strength	1052:1065	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	0	69	theme	cotton	28:33	arg1	fabrics					35:41	cotton fabrics	28:41	cotton fabrics	28:41	Ecofriendly pretreatment of cotton fabrics by ultrasonication and reusing bath chemicals.					
36564693	9	70	theme	color	1749:1753	arg1	degradation					1755:1765	no color degradation	1746:1765	no color degradation	1746:1765	The results also revealed that there was no color degradation during ultrasonication.					
36564693	8	71	theme	Color	1500:1504	arg1	fastness					1506:1513	Color fastness	1500:1513	Color fastness to light, washing, perspiration, and rubbing	1500:1558	Color fastness to light, washing, perspiration, and rubbing were found to be comparable to the conventional technique for low temperature ultrasonicated pretreated and reuse-1 pretreated dyed knit fabric.					
36564693	10	72	theme	scanning	1814:1821	arg1	microscopy					1832:1841	scanning electron microscopy	1814:1841	scanning electron microscopy (SEM)	1814:1847	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	10	72	theme	scanning	1814:1821	arg1	SEM					1844:1846	SEM	1844:1846	SEM	1844:1846	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	3	73	theme	wet	410:412	arg1	processing					414:423	Textile wet processing	402:423	Textile wet processing	402:423	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	10	74	from	changes	1873:1879	arg1	composition					1897:1907	the chemical composition	1884:1907	the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication	1884:2004	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	4	75	theme	wet	787:789	arg1	processing					791:800	wet processing	787:800	wet processing in the textile industry	787:824	The cotton knit fabric used in this study was pretreated and dyed utilizing ultrasonication at a lower temperature than conventional pretreatment and dyeing techniques in an attempt to establish ecofriendly wet processing in the textile industry.					
36564693	3	76	theme	utilities	443:451	arg1	utilities					443:451	utilities	443:451	utilities (water and energy)	443:470	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	3	76	theme	utilities	443:451	arg1	lot					436:438	a lot	434:438	a lot of utilities (water and energy)	434:470	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	3	76	theme	utilities	443:451	arg1	energy					464:469	energy	464:469	energy	464:469	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	3	76	theme	utilities	443:451	arg1	water					454:458	water	454:458	water	454:458	Textile wet processing consumes a lot of utilities (water and energy), and the water generates a lot of waste, which enhances chemical consumption and effluent management costs.					
36564693	5	77	theme	dimensional	1086:1096	arg1	stability					1098:1106	dimensional stability	1086:1106	dimensional stability	1086:1106	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	7	78	with	acceptable	1397:1406	arg1	loss					1436:1439	relatively low weight loss	1414:1439	relatively low weight loss	1414:1439	The sonicated scoured and bleached fabric's whiteness index was found to be acceptable, with relatively low weight loss; however, the bursting strength was found to be increased.					
36564693	7	79	theme	bursting	1455:1462	arg1	strength					1464:1471	the bursting strength	1451:1471	the bursting strength	1451:1471	The sonicated scoured and bleached fabric's whiteness index was found to be acceptable, with relatively low weight loss; however, the bursting strength was found to be increased.					
36564693	2	80	theme	cotton	299:304	arg1	dyeing					311:316	cotton knit dyeing	299:316	cotton knit dyeing	299:316	The quest of new technologies for efficient water and energy use in cotton knit dyeing could result in significant water savings and improve environmental sustainability.					
36564693	1	81	theme	annual	216:221	arg1	export					223:228	total annual export	210:228	total annual export	210:228	The textile industries of Bangladesh contribute significantly to the country's economy, accounting for more than 40% of total annual export.					
36564693	5	82	theme	weight	963:968	arg1	loss					970:973	weight loss	963:973	weight loss	963:973	The bath chemicals were reused up to two times before dyeing in conventional techniques, and fabric properties such as whiteness index, weight loss, bursting strength, color fastness to light, washing, perspiration, rubbing, color strength and durability, or dimensional stability were evaluated and compared with the values obtained by conventional techniques.					
36564693	4	83	theme	cotton	584:589	arg1	fabric					596:601	The cotton knit fabric	580:601	The cotton knit fabric used in this study	580:620	The cotton knit fabric used in this study was pretreated and dyed utilizing ultrasonication at a lower temperature than conventional pretreatment and dyeing techniques in an attempt to establish ecofriendly wet processing in the textile industry.					
36564693	10	84	theme	significant	1861:1871	arg1	changes					1873:1879	no significant changes	1858:1879	no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication	1858:2004	FT-IR spectroscopy and scanning electron microscopy (SEM) revealed no significant changes in the chemical composition of cellulose or the fabric shape of pretreated and dyed cotton knit fabric after ultrasonication.					
36564693	2	85	theme	significant	334:344	arg1	savings					352:358	significant water savings	334:358	significant water savings	334:358	The quest of new technologies for efficient water and energy use in cotton knit dyeing could result in significant water savings and improve environmental sustainability.					
36564693	8	86	theme	conventional	1595:1606	arg1	technique					1608:1616	the conventional technique	1591:1616	the conventional technique for low temperature ultrasonicated pretreated and reuse-1 pretreated dyed knit fabric	1591:1702	Color fastness to light, washing, perspiration, and rubbing were found to be comparable to the conventional technique for low temperature ultrasonicated pretreated and reuse-1 pretreated dyed knit fabric.					
36446650	8	0	theme	cell	1231:1234	arg1	toxicity					1236:1243	cell toxicity	1231:1243	cell toxicity	1231:1243	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.					
36446650	5	1	theme	dentin	767:772	arg1	properties					741:750	mechanical properties	730:750	mechanical properties	730:750	The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized.					
36446650	5	1	theme	dentin	767:772	arg1	composition					713:723	composition	713:723	composition	713:723	The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized.					
36446650	5	1	theme	dentin	767:772	arg1	morphology					701:710	morphology	701:710	morphology	701:710	The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized.					
36446650	5	2	theme	conditioned	755:765	arg1	dentin					767:772	conditioned dentin	755:772	conditioned dentin	755:772	The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized.					
36446650	6	3	theme	adhesive	901:908	arg1	restoration					910:920	adhesive restoration	901:920	adhesive restoration using the dry-bonding technique	901:952	To evaluate dentin bonding performance, SCMC- and PA- conditioned dentin were applied with adhesive restoration using the dry-bonding technique.					
36446650	2	4	theme	demineralization	342:357	arg1	conditioner					359:369	The SCMC-based extrafibrillar demineralization conditioner	312:369	The SCMC-based extrafibrillar demineralization conditioner	312:369	METHODS The SCMC-based extrafibrillar demineralization conditioner was facilely developed by dissolving SCMC into deionized water at an appropriate concentration.					
36446650	7	5	theme	bond	972:975	arg1	MTBS					987:990	MTBS	987:990	MTBS	987:990	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	7	5	theme	bond	972:975	arg1	strength					977:984	The microtensile bond strength	955:984	The microtensile bond strength (MTBS)	955:991	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	14	6	theme	minimal	1874:1880	arg1	intervention					1882:1893	minimal intervention	1874:1893	minimal intervention	1874:1893	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	2	7	theme	SCMC-based	316:325	arg1	conditioner					359:369	The SCMC-based extrafibrillar demineralization conditioner	312:369	The SCMC-based extrafibrillar demineralization conditioner	312:369	METHODS The SCMC-based extrafibrillar demineralization conditioner was facilely developed by dissolving SCMC into deionized water at an appropriate concentration.					
36446650	1	8	theme	extrafibrillar	194:207	arg1	conditioner					226:236	a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner	148:236	a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner	148:236	OBJECTIVE To investigate the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability and explore the possible mechanisms.					
36446650	1	9	theme	conditioner	226:236	arg1	effects					137:143	the effects	133:143	the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability	133:265	OBJECTIVE To investigate the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability and explore the possible mechanisms.					
36446650	1	10	theme	dentin	241:246	arg1	durability					256:265	dentin bonding durability	241:265	dentin bonding durability	241:265	OBJECTIVE To investigate the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability and explore the possible mechanisms.					
36446650	14	11	from	perspective	1859:1869	arg1	SIGNIFICANCE					1837:1848	SIGNIFICANCE	1837:1848	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner	1837:1953	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	9	12	theme	minerals	1342:1349	arg1	retention					1355:1363	retention	1355:1363	retention of intrafibrillar minerals	1355:1390	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	9	12	theme	minerals	1342:1349	arg1	demineralization					1307:1322	demineralization	1307:1322	demineralization of extrafibrillar minerals	1307:1349	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	14	13	theme	adhesive	2058:2065	arg1	restorations					2067:2078	adhesive restorations	2058:2078	adhesive restorations	2058:2078	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	7	14	theme	aging	1132:1136	arg1	month					1111:1115	one month	1107:1115	one month of collagenase aging	1107:1136	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	7	14	theme	aging	1132:1136	arg1	24 h					1060:1063	24 h	1060:1063	24 h of water storage	1060:1080	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	7	14	theme	aging	1132:1136	arg1	thermocycles					1090:1101	10,000 thermocycles	1083:1101	10,000 thermocycles	1083:1101	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	11	15	theme	nanoleakage	1623:1633	arg1	expression					1635:1644	less nanoleakage expression	1618:1644	less nanoleakage expression	1618:1644	Compared with PA, SCMC conditioning produced greater MTBS values and less nanoleakage expression after aging.					
36446650	9	16	theme	intrafibrillar	1368:1381	arg1	minerals					1383:1390	intrafibrillar minerals	1368:1390	intrafibrillar minerals	1368:1390	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	0	17	theme	bonding	88:94	arg1	durability					96:105	dentin bonding durability	81:105	dentin bonding durability	81:105	Sodium carboxymethyl cellulose-based extrafibrillar demineralization to optimize dentin bonding durability.					
36446650	7	18	theme	water	1068:1072	arg1	storage					1074:1080	water storage	1068:1080	water storage	1068:1080	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	12	19	theme	SCMC-conditioned	1711:1726	arg1	dentin					1728:1733	SCMC-conditioned dentin	1711:1733	SCMC-conditioned dentin	1711:1733	Endogenous gelatinolytic activity was suppressed in SCMC-conditioned dentin.					
36446650	4	20	theme	human	617:621	arg1	molars					629:634	human third molars	617:634	human third molars	617:634	Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA).					
36446650	14	21	theme	promising	2009:2017	arg1	strategy					2019:2026	a promising strategy	2007:2026	a promising strategy to extend the service life of adhesive restorations	2007:2078	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	12	22	theme	Endogenous	1659:1668	arg1	activity					1684:1691	Endogenous gelatinolytic activity	1659:1691	Endogenous gelatinolytic activity	1659:1691	Endogenous gelatinolytic activity was suppressed in SCMC-conditioned dentin.					
36446650	4	23	theme	molars	629:634	arg1	surfaces					605:612	Dentin surfaces	598:612	Dentin surfaces of human third molars	598:634	Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA).					
36446650	10	24	theme	SCMC-conditioned	1501:1516	arg1	dentin					1518:1523	SCMC-conditioned dentin	1501:1523	SCMC-conditioned dentin	1501:1523	The mechanical properties of SCMC-conditioned dentin were largely preserved.					
36446650	9	25	theme	single	1413:1418	arg1	model					1435:1439	the single layer collagen model	1409:1439	the single layer collagen model	1409:1439	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	11	26	theme	SCMC	1567:1570	arg1	conditioning					1572:1583	SCMC conditioning	1567:1583	SCMC conditioning	1567:1583	Compared with PA, SCMC conditioning produced greater MTBS values and less nanoleakage expression after aging.					
36446650	9	27	theme	collagen	1426:1433	arg1	model					1435:1439	the single layer collagen model	1409:1439	the single layer collagen model	1409:1439	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	0	28	theme	Sodium	0:5	arg1	carboxymethyl					7:19	Sodium carboxymethyl	0:19	Sodium carboxymethyl	0:19	Sodium carboxymethyl cellulose-based extrafibrillar demineralization to optimize dentin bonding durability.					
36446650	8	29	theme	inhibitory	1143:1152	arg1	effect					1154:1159	The inhibitory effect	1139:1159	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity	1139:1243	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.					
36446650	7	30	theme	in	1021:1022	arg1	zymography					1029:1038	in situ zymography	1021:1038	in situ zymography	1021:1038	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	11	31	theme	MTBS	1602:1605	arg1	values					1607:1612	greater MTBS values	1594:1612	greater MTBS values	1594:1612	Compared with PA, SCMC conditioning produced greater MTBS values and less nanoleakage expression after aging.					
36446650	9	32	theme	dentin	1449:1454	arg1	ultrastructure					1456:1469	the dentin ultrastructure	1445:1469	the dentin ultrastructure	1445:1469	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	3	33	theme	mineralization/demineralization	491:521	arg1	model					523:527	A single layer collagen mineralization/demineralization model	467:527	A single layer collagen mineralization/demineralization model	467:527	A single layer collagen mineralization/demineralization model was designed to visualize extrafibrillar demineralization in detail.					
36446650	4	34	theme	37	670:671	arg1	%					673:673	%	673:673	%	673:673	Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA).					
36446650	3	35	theme	extrafibrillar	555:568	arg1	demineralization					570:585	extrafibrillar demineralization	555:585	extrafibrillar demineralization	555:585	A single layer collagen mineralization/demineralization model was designed to visualize extrafibrillar demineralization in detail.					
36446650	14	36	theme	bonding	1976:1982	arg1	durability					1984:1993	dentin bonding durability	1969:1993	dentin bonding durability	1969:1993	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	3	37	theme	layer	476:480	arg1	model					523:527	A single layer collagen mineralization/demineralization model	467:527	A single layer collagen mineralization/demineralization model	467:527	A single layer collagen mineralization/demineralization model was designed to visualize extrafibrillar demineralization in detail.					
36446650	2	38	theme	deionized	418:426	arg1	water					428:432	deionized water	418:432	deionized water at an appropriate concentration	418:464	METHODS The SCMC-based extrafibrillar demineralization conditioner was facilely developed by dissolving SCMC into deionized water at an appropriate concentration.					
36446650	8	39	theme	human	1184:1188	arg1	rhMMP-2					1218:1224	rhMMP-2	1218:1224	rhMMP-2	1218:1224	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.					
36446650	8	39	theme	human	1184:1188	arg1	metalloproteinase-2					1197:1215	recombinant human matrix metalloproteinase-2	1172:1215	recombinant human matrix metalloproteinase-2 (rhMMP-2)	1172:1225	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.					
36446650	4	40	theme	3	658:658	arg1	%					660:660	%	660:660	%	660:660	Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA).					
36446650	13	41	theme	rhMMP-2	1785:1791	arg1	inhibition					1771:1780	the inhibition	1767:1780	the inhibition of rhMMP-2 by SCMC	1767:1799	In addition to being nontoxic, the inhibition of rhMMP-2 by SCMC was confirmed to be dose-dependent.					
36446650	13	41	theme	rhMMP-2	1785:1791	arg1	dose-dependent					1821:1834	dose-dependent	1821:1834	dose-dependent	1821:1834	In addition to being nontoxic, the inhibition of rhMMP-2 by SCMC was confirmed to be dose-dependent.					
36446650	1	42	from	effects	137:143	arg1	durability					256:265	dentin bonding durability	241:265	dentin bonding durability	241:265	OBJECTIVE To investigate the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability and explore the possible mechanisms.					
36446650	6	43	theme	dry-bonding	932:942	arg1	technique					944:952	the dry-bonding technique	928:952	the dry-bonding technique	928:952	To evaluate dentin bonding performance, SCMC- and PA- conditioned dentin were applied with adhesive restoration using the dry-bonding technique.					
36446650	13	44	dep	nontoxic	1757:1764	arg1	addition					1739:1746	addition	1739:1746	addition	1739:1746	In addition to being nontoxic, the inhibition of rhMMP-2 by SCMC was confirmed to be dose-dependent.					
36446650	10	45	theme	mechanical	1476:1485	arg1	properties					1487:1496	The mechanical properties	1472:1496	The mechanical properties of SCMC-conditioned dentin	1472:1523	The mechanical properties of SCMC-conditioned dentin were largely preserved.					
36446650	2	46	theme	appropriate	440:450	arg1	concentration					452:464	an appropriate concentration	437:464	an appropriate concentration	437:464	METHODS The SCMC-based extrafibrillar demineralization conditioner was facilely developed by dissolving SCMC into deionized water at an appropriate concentration.					
36446650	14	47	theme	extrafibrillar	1911:1924	arg1	perspective					1859:1869	the perspective	1855:1869	the perspective of minimal intervention	1855:1893	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	14	47	theme	extrafibrillar	1911:1924	arg1	conditioner					1943:1953	the SCMC-based extrafibrillar demineralization conditioner	1896:1953	the SCMC-based extrafibrillar demineralization conditioner	1896:1953	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	5	48	theme	mechanical	730:739	arg1	properties					741:750	mechanical properties	730:750	mechanical properties	730:750	The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized.					
36446650	4	49	theme	phosphoric	675:684	arg1	PA					692:693	PA	692:693	PA	692:693	Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA).					
36446650	4	49	theme	phosphoric	675:684	arg1	acid					686:689	37 % phosphoric acid	670:689	37 % phosphoric acid (PA)	670:694	Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA).					
36446650	10	50	theme	dentin	1518:1523	arg1	properties					1487:1496	The mechanical properties	1472:1496	The mechanical properties of SCMC-conditioned dentin	1472:1523	The mechanical properties of SCMC-conditioned dentin were largely preserved.					
36446650	14	51	theme	intervention	1882:1893	arg1	perspective					1859:1869	the perspective	1855:1869	the perspective of minimal intervention	1855:1893	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	14	51	theme	intervention	1882:1893	arg1	conditioner					1943:1953	the SCMC-based extrafibrillar demineralization conditioner	1896:1953	the SCMC-based extrafibrillar demineralization conditioner	1896:1953	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	9	52	located	observed	1397:1404	arg2	demineralization					1307:1322	demineralization	1307:1322	demineralization of extrafibrillar minerals	1307:1349	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	9	52	located	observed	1397:1404	arg1	ultrastructure					1456:1469	the dentin ultrastructure	1445:1469	the dentin ultrastructure	1445:1469	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	9	52	located	observed	1397:1404	arg2	retention					1355:1363	retention	1355:1363	retention of intrafibrillar minerals	1355:1390	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	9	52	located	observed	1397:1404	arg1	model					1435:1439	the single layer collagen model	1409:1439	the single layer collagen model	1409:1439	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	9	53	theme	SCMC	1283:1286	arg1	conditioning					1288:1299	SCMC conditioning	1283:1299	SCMC conditioning	1283:1299	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	2	54	theme	extrafibrillar	327:340	arg1	conditioner					359:369	The SCMC-based extrafibrillar demineralization conditioner	312:369	The SCMC-based extrafibrillar demineralization conditioner	312:369	METHODS The SCMC-based extrafibrillar demineralization conditioner was facilely developed by dissolving SCMC into deionized water at an appropriate concentration.					
36446650	1	55	theme	-based	187:192	arg1	conditioner					226:236	a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner	148:236	a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner	148:236	OBJECTIVE To investigate the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability and explore the possible mechanisms.					
36446650	7	56	theme	microtensile	959:970	arg1	MTBS					987:990	MTBS	987:990	MTBS	987:990	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	7	56	theme	microtensile	959:970	arg1	strength					977:984	The microtensile bond strength	955:984	The microtensile bond strength (MTBS)	955:991	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	1	57	theme	demineralization	209:224	arg1	conditioner					226:236	a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner	148:236	a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner	148:236	OBJECTIVE To investigate the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability and explore the possible mechanisms.					
36446650	2	58	dep	METHODS	304:310	arg1	developed					384:392	developed	384:392	was facilely developed by dissolving SCMC into deionized water at an appropriate concentration	371:464	METHODS The SCMC-based extrafibrillar demineralization conditioner was facilely developed by dissolving SCMC into deionized water at an appropriate concentration.					
36446650	6	59	theme	conditioned	864:874	arg1	dentin					876:881	SCMC- and PA- conditioned dentin	850:881	SCMC- and PA- conditioned dentin	850:881	To evaluate dentin bonding performance, SCMC- and PA- conditioned dentin were applied with adhesive restoration using the dry-bonding technique.					
36446650	9	60	theme	extrafibrillar	1327:1340	arg1	minerals					1342:1349	extrafibrillar minerals	1327:1349	extrafibrillar minerals	1327:1349	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	1	61	theme	bonding	248:254	arg1	durability					256:265	dentin bonding durability	241:265	dentin bonding durability	241:265	OBJECTIVE To investigate the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability and explore the possible mechanisms.					
36446650	8	62	from	effect	1154:1159	arg1	rhMMP-2					1218:1224	rhMMP-2	1218:1224	rhMMP-2	1218:1224	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.					
36446650	8	62	from	effect	1154:1159	arg1	metalloproteinase-2					1197:1215	recombinant human matrix metalloproteinase-2	1172:1215	recombinant human matrix metalloproteinase-2 (rhMMP-2)	1172:1225	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.					
36446650	8	62	from	effect	1154:1159	arg1	toxicity					1236:1243	cell toxicity	1231:1243	cell toxicity	1231:1243	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.					
36446650	7	63	theme	collagenase	1120:1130	arg1	aging					1132:1136	collagenase aging	1120:1136	collagenase aging	1120:1136	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	14	64	theme	service	2042:2048	arg1	life					2050:2053	the service life	2038:2053	the service life of adhesive restorations	2038:2078	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	9	65	theme	minerals	1383:1390	arg1	retention					1355:1363	retention	1355:1363	retention of intrafibrillar minerals	1355:1390	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	9	65	theme	minerals	1383:1390	arg1	demineralization					1307:1322	demineralization	1307:1322	demineralization of extrafibrillar minerals	1307:1349	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	9	66	dep	RESULTS	1269:1275	arg1	conditioning					1288:1299	SCMC conditioning	1283:1299	SCMC conditioning	1283:1299	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	4	67	theme	third	623:627	arg1	molars					629:634	human third molars	617:634	human third molars	617:634	Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA).					
36446650	7	68	dep	in	1021:1022	arg1	situ					1024:1027	situ	1024:1027	situ	1024:1027	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	7	69	theme	storage	1074:1080	arg1	month					1111:1115	one month	1107:1115	one month of collagenase aging	1107:1136	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	7	69	theme	storage	1074:1080	arg1	24 h					1060:1063	24 h	1060:1063	24 h of water storage	1060:1080	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	7	69	theme	storage	1074:1080	arg1	thermocycles					1090:1101	10,000 thermocycles	1083:1101	10,000 thermocycles	1083:1101	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	12	70	theme	gelatinolytic	1670:1682	arg1	activity					1684:1691	Endogenous gelatinolytic activity	1659:1691	Endogenous gelatinolytic activity	1659:1691	Endogenous gelatinolytic activity was suppressed in SCMC-conditioned dentin.					
36446650	4	71	theme	Dentin	598:603	arg1	surfaces					605:612	Dentin surfaces	598:612	Dentin surfaces of human third molars	598:634	Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA).					
36446650	9	72	theme	layer	1420:1424	arg1	model					1435:1439	the single layer collagen model	1409:1439	the single layer collagen model	1409:1439	RESULTS After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure.					
36446650	14	73	theme	demineralization	1926:1941	arg1	perspective					1859:1869	the perspective	1855:1869	the perspective of minimal intervention	1855:1893	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	14	73	theme	demineralization	1926:1941	arg1	conditioner					1943:1953	the SCMC-based extrafibrillar demineralization conditioner	1896:1953	the SCMC-based extrafibrillar demineralization conditioner	1896:1953	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	3	74	theme	single	469:474	arg1	model					523:527	A single layer collagen mineralization/demineralization model	467:527	A single layer collagen mineralization/demineralization model	467:527	A single layer collagen mineralization/demineralization model was designed to visualize extrafibrillar demineralization in detail.					
36446650	11	75	theme	greater	1594:1600	arg1	values					1607:1612	greater MTBS values	1594:1612	greater MTBS values	1594:1612	Compared with PA, SCMC conditioning produced greater MTBS values and less nanoleakage expression after aging.					
36446650	0	76	theme	extrafibrillar	37:50	arg1	demineralization					52:67	extrafibrillar demineralization	37:67	extrafibrillar demineralization	37:67	Sodium carboxymethyl cellulose-based extrafibrillar demineralization to optimize dentin bonding durability.					
36446650	8	77	theme	SCMC	1164:1167	arg1	effect					1154:1159	The inhibitory effect	1139:1159	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity	1139:1243	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.					
36446650	6	78	theme	bonding	829:835	arg1	performance					837:847	dentin bonding performance	822:847	dentin bonding performance	822:847	To evaluate dentin bonding performance, SCMC- and PA- conditioned dentin were applied with adhesive restoration using the dry-bonding technique.					
36446650	4	79	theme	%	673:673	arg1	PA					692:693	PA	692:693	PA	692:693	Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA).					
36446650	4	79	theme	%	673:673	arg1	acid					686:689	37 % phosphoric acid	670:689	37 % phosphoric acid (PA)	670:694	Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA).					
36446650	8	80	theme	recombinant	1172:1182	arg1	rhMMP-2					1218:1224	rhMMP-2	1218:1224	rhMMP-2	1218:1224	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.					
36446650	8	80	theme	recombinant	1172:1182	arg1	metalloproteinase-2					1197:1215	recombinant human matrix metalloproteinase-2	1172:1215	recombinant human matrix metalloproteinase-2 (rhMMP-2)	1172:1225	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.					
36446650	14	81	theme	restorations	2067:2078	arg1	life					2050:2053	the service life	2038:2053	the service life of adhesive restorations	2038:2078	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	0	82	theme	dentin	81:86	arg1	durability					96:105	dentin bonding durability	81:105	dentin bonding durability	81:105	Sodium carboxymethyl cellulose-based extrafibrillar demineralization to optimize dentin bonding durability.					
36446650	14	83	theme	dentin	1969:1974	arg1	durability					1984:1993	dentin bonding durability	1969:1993	dentin bonding durability	1969:1993	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	8	84	theme	matrix	1190:1195	arg1	rhMMP-2					1218:1224	rhMMP-2	1218:1224	rhMMP-2	1218:1224	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.					
36446650	8	84	theme	matrix	1190:1195	arg1	metalloproteinase-2					1197:1215	recombinant human matrix metalloproteinase-2	1172:1215	recombinant human matrix metalloproteinase-2 (rhMMP-2)	1172:1225	The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.					
36446650	2	85	from	concentration	452:464	arg1	water					428:432	deionized water	418:432	deionized water at an appropriate concentration	418:464	METHODS The SCMC-based extrafibrillar demineralization conditioner was facilely developed by dissolving SCMC into deionized water at an appropriate concentration.					
36446650	3	86	theme	collagen	482:489	arg1	model					523:527	A single layer collagen mineralization/demineralization model	467:527	A single layer collagen mineralization/demineralization model	467:527	A single layer collagen mineralization/demineralization model was designed to visualize extrafibrillar demineralization in detail.					
36446650	4	87	theme	%	660:660	arg1	SCMC					662:665	3 % SCMC	658:665	3 % SCMC	658:665	Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA).					
36446650	6	88	theme	SCMC-	850:854	arg1	dentin					876:881	SCMC- and PA- conditioned dentin	850:881	SCMC- and PA- conditioned dentin	850:881	To evaluate dentin bonding performance, SCMC- and PA- conditioned dentin were applied with adhesive restoration using the dry-bonding technique.					
36446650	5	89	dep	morphology	701:710	arg1	The					697:699	The	697:699	The	697:699	The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized.					
36446650	7	90	theme	interface	994:1002	arg1	nanoleakage					1004:1014	interface nanoleakage	994:1014	interface nanoleakage	994:1014	The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging.					
36446650	5	91	from	group	784:788	arg1	properties					741:750	mechanical properties	730:750	mechanical properties	730:750	The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized.					
36446650	5	91	from	group	784:788	arg1	composition					713:723	composition	713:723	composition	713:723	The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized.					
36446650	5	91	from	group	784:788	arg1	morphology					701:710	morphology	701:710	morphology	701:710	The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized.					
36446650	14	92	theme	SCMC-based	1900:1909	arg1	perspective					1859:1869	the perspective	1855:1869	the perspective of minimal intervention	1855:1893	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	14	92	theme	SCMC-based	1900:1909	arg1	conditioner					1943:1953	the SCMC-based extrafibrillar demineralization conditioner	1896:1953	the SCMC-based extrafibrillar demineralization conditioner	1896:1953	SIGNIFICANCE From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.					
36446650	6	93	theme	dentin	822:827	arg1	performance					837:847	dentin bonding performance	822:847	dentin bonding performance	822:847	To evaluate dentin bonding performance, SCMC- and PA- conditioned dentin were applied with adhesive restoration using the dry-bonding technique.					
36446650	1	94	theme	possible	283:290	arg1	mechanisms					292:301	the possible mechanisms	279:301	the possible mechanisms	279:301	OBJECTIVE To investigate the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability and explore the possible mechanisms.					
36174982	11	0	from	effects	1832:1838	arg1	liver					1864:1868	liver	1864:1868	liver	1864:1868	Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver.					
36174982	11	0	from	effects	1832:1838	arg1	tissue					1853:1858	the renal tissue	1843:1858	the renal tissue	1843:1858	Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver.					
36174982	7	1	from	h	1257:1257	arg1	release					1227:1233	the sustained release	1213:1233	the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline	1213:1293	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	7	1	from	h	1257:1257	arg1	pH					1262:1263	pH 5.5 phosphate-buffered saline	1262:1293	pH 5.5 phosphate-buffered saline	1262:1293	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	7	1	from	h	1257:1257	arg1	%					1249:1249	72.2% ± 1.8%	1238:1249	72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline	1238:1293	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	2	2	theme	-sodium	286:292	arg1	nanogels					327:334	florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels	254:334	florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels	254:334	OBJECTIVES In this study, florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels were prepared to allow targeted delivery to SCV infected sites.					
36174982	5	3	theme	±	855:855	arg1	%					860:860	5.8% ± 1.4%	850:860	5.8% ± 1.4%	850:860	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	6	4	theme	polymeric	1085:1093	arg1	networks					1095:1102	cross-linked polymeric networks	1072:1102	cross-linked polymeric networks	1072:1102	Optical and scanning electron microscopy showed that spherical particles with a relatively uniform distribution and drugs might be incorporated in cross-linked polymeric networks.					
36174982	5	5	theme	loading	718:724	arg1	capacity					726:733	loading capacity	718:733	loading capacity	718:733	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	6	6	theme	uniform	1016:1022	arg1	distribution					1024:1035	a relatively uniform distribution	1003:1035	a relatively uniform distribution	1003:1035	Optical and scanning electron microscopy showed that spherical particles with a relatively uniform distribution and drugs might be incorporated in cross-linked polymeric networks.					
36174982	7	7	dep	pH	1262:1263	arg1	saline					1288:1293	phosphate-buffered saline	1269:1293	pH 5.5 phosphate-buffered saline	1262:1293	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	8	8	theme	composite	1385:1393	arg1	nanogels					1395:1402	commercial florfenicol solution and florfenicol composite nanogels	1337:1402	nanogels	1395:1402	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 µg/mL, respectively.					
36174982	5	9	theme	1.5	871:873	arg1	±					869:869	±	869:869	±	869:869	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	3	10	theme	nanogels	563:570	arg1	METHODS					400:406	METHODS	400:406	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels	400:570	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	10	theme	nanogels	563:570	arg1	biosafety					524:532	biosafety	524:532	biosafety	524:532	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	10	theme	nanogels	563:570	arg1	efficacy					510:517	therapeutic efficacy	498:517	therapeutic efficacy	498:517	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	10	theme	nanogels	563:570	arg1	screening					424:432	The formulation screening	408:432	The formulation screening	408:432	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	10	theme	nanogels	563:570	arg1	release					465:471	in vitro release	456:471	in vitro release	456:471	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	10	theme	nanogels	563:570	arg1	activity					488:495	antibacterial activity	474:495	antibacterial activity	474:495	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	6	11	theme	scanning	937:944	arg1	microscopy					955:964	Optical and scanning electron microscopy	925:964	microscopy	955:964	Optical and scanning electron microscopy showed that spherical particles with a relatively uniform distribution and drugs might be incorporated in cross-linked polymeric networks.					
36174982	7	12	theme	±	1244:1244	arg1	%					1249:1249	72.2% ± 1.8%	1238:1249	72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline	1238:1293	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	11	13	theme	florfenicol	1737:1747	arg1	nanogels					1759:1766	the florfenicol composite nanogels	1733:1766	the florfenicol composite nanogels	1733:1766	Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver.					
36174982	11	13	theme	florfenicol	1737:1747	arg1	preparation					1793:1803	a biocompatible preparation	1777:1803	a biocompatible preparation	1777:1803	Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver.					
36174982	0	14	theme	aureus	80:85	arg1	variants					100:107	Staphylococcus aureus small colony variants	65:107	Staphylococcus aureus small colony variants	65:107	Antibacterial activity of florfenicol composite nanogels against Staphylococcus aureus small colony variants.					
36174982	2	15	theme	chitosan	273:280	arg1	nanogels					327:334	florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels	254:334	florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels	254:334	OBJECTIVES In this study, florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels were prepared to allow targeted delivery to SCV infected sites.					
36174982	9	16	theme	time-killing	1458:1469	arg1	curves					1471:1476	The time-killing curves	1454:1476	The time-killing curves	1454:1476	The time-killing curves and live-dead bacterial staining showed that the florfenicol composite nanogels were concentration-dependent.					
36174982	6	17	theme	Optical	925:931	arg1	microscopy					955:964	Optical and scanning electron microscopy	925:964	microscopy	955:964	Optical and scanning electron microscopy showed that spherical particles with a relatively uniform distribution and drugs might be incorporated in cross-linked polymeric networks.					
36174982	11	18	theme	biocompatible	1779:1791	arg1	nanogels					1759:1766	the florfenicol composite nanogels	1733:1766	the florfenicol composite nanogels	1733:1766	Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver.					
36174982	11	18	theme	biocompatible	1779:1791	arg1	preparation					1793:1803	a biocompatible preparation	1777:1803	a biocompatible preparation	1777:1803	Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver.					
36174982	0	19	theme	colony	93:98	arg1	variants					100:107	Staphylococcus aureus small colony variants	65:107	Staphylococcus aureus small colony variants	65:107	Antibacterial activity of florfenicol composite nanogels against Staphylococcus aureus small colony variants.					
36174982	1	20	theme	colony	195:200	arg1	SCVs					212:215	SCVs	212:215	SCVs	212:215	BACKGROUND Florfenicol might be ineffective for treating Staphylococcus aureus small colony variants (SCVs) mastitis.					
36174982	1	20	theme	colony	195:200	arg1	variants					202:209	Staphylococcus aureus small colony variants	167:209	Staphylococcus aureus small colony variants (SCVs) mastitis	167:225	BACKGROUND Florfenicol might be ineffective for treating Staphylococcus aureus small colony variants (SCVs) mastitis.					
36174982	3	21	dep	METHODS	400:406	arg1	METHODS					400:406	METHODS	400:406	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels	400:570	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	21	dep	METHODS	400:406	arg1	biosafety					524:532	biosafety	524:532	biosafety	524:532	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	21	dep	METHODS	400:406	arg1	characteristics					439:453	the characteristics	435:453	the characteristics	435:453	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	21	dep	METHODS	400:406	arg1	efficacy					510:517	therapeutic efficacy	498:517	therapeutic efficacy	498:517	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	21	dep	METHODS	400:406	arg1	screening					424:432	The formulation screening	408:432	The formulation screening	408:432	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	21	dep	METHODS	400:406	arg1	release					465:471	in vitro release	456:471	in vitro release	456:471	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	21	dep	METHODS	400:406	arg1	activity					488:495	antibacterial activity	474:495	antibacterial activity	474:495	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	12	22	theme	infections	1949:1958	arg1	treatment					1932:1940	the treatment	1928:1940	the treatment of SCV infections	1928:1958	CONCLUSIONS Florfenicol composite nanogels might improve the treatment of SCV infections.					
36174982	9	23	theme	bacterial	1492:1500	arg1	staining					1502:1509	live-dead bacterial staining	1482:1509	live-dead bacterial staining	1482:1509	The time-killing curves and live-dead bacterial staining showed that the florfenicol composite nanogels were concentration-dependent.					
36174982	3	24	theme	in	456:457	arg1	METHODS					400:406	METHODS	400:406	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels	400:570	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	24	theme	in	456:457	arg1	release					465:471	in vitro release	456:471	in vitro release	456:471	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	5	25	theme	280.3	863:867	arg1	±					869:869	±	869:869	±	869:869	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	0	26	theme	Antibacterial	0:12	arg1	activity					14:21	Antibacterial activity	0:21	Antibacterial activity of florfenicol composite	0:46	Antibacterial activity of florfenicol composite nanogels against Staphylococcus aureus small colony variants.					
36174982	1	27	theme	BACKGROUND	110:119	arg1	Florfenicol					121:131	BACKGROUND Florfenicol	110:131	BACKGROUND Florfenicol	110:131	BACKGROUND Florfenicol might be ineffective for treating Staphylococcus aureus small colony variants (SCVs) mastitis.					
36174982	11	28	theme	safety	1706:1711	arg1	studies					1713:1719	Biological safety studies	1695:1719	Biological safety studies	1695:1719	Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver.					
36174982	8	29	theme	minimal	1300:1306	arg1	µg/mL					1433:1437	1 and 0.25 µg/mL	1422:1437	1 and 0.25 µg/mL	1422:1437	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 µg/mL, respectively.					
36174982	8	29	theme	minimal	1300:1306	arg1	concentrations					1319:1332	The minimal inhibitory concentrations	1296:1332	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs	1296:1415	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 µg/mL, respectively.					
36174982	3	30	theme	antibacterial	474:486	arg1	METHODS					400:406	METHODS	400:406	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels	400:570	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	30	theme	antibacterial	474:486	arg1	activity					488:495	antibacterial activity	474:495	antibacterial activity	474:495	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	7	31	theme	in	1109:1110	arg1	study					1126:1130	The in vitro release study	1105:1130	The in vitro release study	1105:1130	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	3	32	theme	florfenicol	541:551	arg1	nanogels					563:570	the florfenicol composite nanogels	537:570	the florfenicol composite nanogels	537:570	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	9	33	theme	composite	1539:1547	arg1	concentration-dependent					1563:1585	concentration-dependent	1563:1585	concentration-dependent	1563:1585	The time-killing curves and live-dead bacterial staining showed that the florfenicol composite nanogels were concentration-dependent.					
36174982	9	33	theme	composite	1539:1547	arg1	nanogels					1549:1556	the florfenicol composite nanogels	1523:1556	the florfenicol composite nanogels	1523:1556	The time-killing curves and live-dead bacterial staining showed that the florfenicol composite nanogels were concentration-dependent.					
36174982	7	34	theme	release	1118:1124	arg1	study					1126:1130	The in vitro release study	1105:1130	The in vitro release study	1105:1130	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	0	35	theme	composite	38:46	arg1	activity					14:21	Antibacterial activity	0:21	Antibacterial activity of florfenicol composite	0:46	Antibacterial activity of florfenicol composite nanogels against Staphylococcus aureus small colony variants.					
36174982	12	36	theme	composite	1895:1903	arg1	nanogels					1905:1912	CONCLUSIONS Florfenicol composite nanogels	1871:1912	CONCLUSIONS Florfenicol composite nanogels	1871:1912	CONCLUSIONS Florfenicol composite nanogels might improve the treatment of SCV infections.					
36174982	7	37	theme	%	1242:1242	arg1	%					1249:1249	72.2% ± 1.8%	1238:1249	72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline	1238:1293	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	3	38	theme	formulation	412:422	arg1	METHODS					400:406	METHODS	400:406	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels	400:570	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	38	theme	formulation	412:422	arg1	screening					424:432	The formulation screening	408:432	The formulation screening	408:432	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	8	39	theme	commercial	1337:1346	arg1	solution					1360:1367	commercial florfenicol solution and florfenicol composite nanogels	1337:1402	solution	1360:1367	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 µg/mL, respectively.					
36174982	2	40	theme	targeted	359:366	arg1	delivery					368:375	targeted delivery	359:375	targeted delivery to SCV infected sites	359:397	OBJECTIVES In this study, florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels were prepared to allow targeted delivery to SCV infected sites.					
36174982	5	41	theme	florfenicol	800:810	arg1	nanogels					822:829	the optimized florfenicol composite nanogels	786:829	the optimized florfenicol composite nanogels	786:829	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	8	42	theme	solution	1360:1367	arg1	µg/mL					1433:1437	1 and 0.25 µg/mL	1422:1437	1 and 0.25 µg/mL	1422:1437	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 µg/mL, respectively.					
36174982	8	42	theme	solution	1360:1367	arg1	concentrations					1319:1332	The minimal inhibitory concentrations	1296:1332	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs	1296:1415	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 µg/mL, respectively.					
36174982	3	43	theme	composite	553:561	arg1	nanogels					563:570	the florfenicol composite nanogels	537:570	the florfenicol composite nanogels	537:570	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	7	44	theme	sustained	1217:1225	arg1	release					1227:1233	the sustained release	1213:1233	the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline	1213:1293	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	8	45	theme	florfenicol	1373:1383	arg1	nanogels					1395:1402	commercial florfenicol solution and florfenicol composite nanogels	1337:1402	nanogels	1395:1402	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 µg/mL, respectively.					
36174982	2	46	theme	infected	384:391	arg1	sites					393:397	SCV infected sites	380:397	SCV infected sites	380:397	OBJECTIVES In this study, florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels were prepared to allow targeted delivery to SCV infected sites.					
36174982	7	47	theme	florfenicol	1148:1158	arg1	nanogels					1170:1177	the florfenicol composite nanogels	1144:1177	the florfenicol composite nanogels	1144:1177	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	7	48	theme	biphasic	1191:1198	arg1	pattern					1200:1206	a biphasic pattern	1189:1206	a biphasic pattern	1189:1206	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	4	49	theme	optimized	598:606	arg1	formulation					608:618	The optimized formulation	594:618	The optimized formulation	594:618	RESULTS The optimized formulation was obtained when the CS and TPP were 10 and 5 mg/mL, respectively.					
36174982	2	50	theme	TPP	312:314	arg1	nanogels					327:334	florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels	254:334	florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels	254:334	OBJECTIVES In this study, florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels were prepared to allow targeted delivery to SCV infected sites.					
36174982	2	51	theme	tripolyphosphate	294:309	arg1	nanogels					327:334	florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels	254:334	florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels	254:334	OBJECTIVES In this study, florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels were prepared to allow targeted delivery to SCV infected sites.					
36174982	1	52	theme	variants	202:209	arg1	mastitis					218:225	Staphylococcus aureus small colony variants (SCVs) mastitis	167:225	Staphylococcus aureus small colony variants (SCVs) mastitis	167:225	BACKGROUND Florfenicol might be ineffective for treating Staphylococcus aureus small colony variants (SCVs) mastitis.					
36174982	4	53	theme	5	665:665	arg1	mg/mL					667:671	10 and 5 mg/mL	658:671	mg/mL	667:671	RESULTS The optimized formulation was obtained when the CS and TPP were 10 and 5 mg/mL, respectively.					
36174982	7	54	theme	composite	1160:1168	arg1	nanogels					1170:1177	the florfenicol composite nanogels	1144:1177	the florfenicol composite nanogels	1144:1177	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	5	55	theme	%	853:853	arg1	%					860:860	5.8% ± 1.4%	850:860	5.8% ± 1.4%	850:860	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	7	56	from	release	1227:1233	arg1	h					1257:1257	48 h	1254:1257	48 h in pH 5.5 phosphate-buffered saline	1254:1293	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	10	57	theme	SCVs	1680:1683	arg1	mastitis					1685:1692	SCVs mastitis	1680:1692	SCVs mastitis	1680:1692	Furthermore, the florfenicol composite nanogels displayed good therapeutic efficacy against SCVs mastitis.					
36174982	2	58	theme	composite	317:325	arg1	nanogels					327:334	florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels	254:334	florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels	254:334	OBJECTIVES In this study, florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels were prepared to allow targeted delivery to SCV infected sites.					
36174982	5	59	theme	%	840:840	arg1	%					847:847	87.3% ± 2.7%	836:847	87.3% ± 2.7%	836:847	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	5	59	theme	%	840:840	arg1	efficiency					706:715	The encapsulation efficiency	688:715	The encapsulation efficiency	688:715	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	8	60	theme	nanogels	1395:1402	arg1	µg/mL					1433:1437	1 and 0.25 µg/mL	1422:1437	1 and 0.25 µg/mL	1422:1437	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 µg/mL, respectively.					
36174982	8	60	theme	nanogels	1395:1402	arg1	concentrations					1319:1332	The minimal inhibitory concentrations	1296:1332	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs	1296:1415	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 µg/mL, respectively.					
36174982	6	61	theme	cross-linked	1072:1083	arg1	networks					1095:1102	cross-linked polymeric networks	1072:1102	cross-linked polymeric networks	1072:1102	Optical and scanning electron microscopy showed that spherical particles with a relatively uniform distribution and drugs might be incorporated in cross-linked polymeric networks.					
36174982	10	62	theme	florfenicol	1605:1615	arg1	nanogels					1627:1634	the florfenicol composite nanogels	1601:1634	the florfenicol composite nanogels	1601:1634	Furthermore, the florfenicol composite nanogels displayed good therapeutic efficacy against SCVs mastitis.					
36174982	2	63	theme	florfenicol-loaded	254:271	arg1	CS					283:284	CS	283:284	CS	283:284	OBJECTIVES In this study, florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels were prepared to allow targeted delivery to SCV infected sites.					
36174982	2	63	theme	florfenicol-loaded	254:271	arg1	chitosan					273:280	florfenicol-loaded chitosan	254:280	florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels	254:334	OBJECTIVES In this study, florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels were prepared to allow targeted delivery to SCV infected sites.					
36174982	4	64	theme	10	658:659	arg1	mg/mL					667:671	10 and 5 mg/mL	658:671	mg/mL	667:671	RESULTS The optimized formulation was obtained when the CS and TPP were 10 and 5 mg/mL, respectively.					
36174982	11	65	theme	renal	1847:1851	arg1	tissue					1853:1858	the renal tissue	1843:1858	the renal tissue	1843:1858	Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver.					
36174982	11	66	theme	non-toxic	1822:1830	arg1	effects					1832:1838	their non-toxic effects	1816:1838	their non-toxic effects on the renal tissue and liver	1816:1868	Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver.					
36174982	5	67	theme	encapsulation	692:704	arg1	%					847:847	87.3% ± 2.7%	836:847	87.3% ± 2.7%	836:847	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	5	67	theme	encapsulation	692:704	arg1	efficiency					706:715	The encapsulation efficiency	688:715	The encapsulation efficiency	688:715	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	4	68	dep	CS	642:643	arg1	the					638:640	the	638:640	the	638:640	RESULTS The optimized formulation was obtained when the CS and TPP were 10 and 5 mg/mL, respectively.					
36174982	6	69	theme	electron	946:953	arg1	microscopy					955:964	Optical and scanning electron microscopy	925:964	microscopy	955:964	Optical and scanning electron microscopy showed that spherical particles with a relatively uniform distribution and drugs might be incorporated in cross-linked polymeric networks.					
36174982	7	70	theme	phosphate-buffered	1269:1286	arg1	saline					1288:1293	phosphate-buffered saline	1269:1293	pH 5.5 phosphate-buffered saline	1262:1293	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	0	71	theme	small	87:91	arg1	variants					100:107	Staphylococcus aureus small colony variants	65:107	Staphylococcus aureus small colony variants	65:107	Antibacterial activity of florfenicol composite nanogels against Staphylococcus aureus small colony variants.					
36174982	11	72	theme	composite	1749:1757	arg1	nanogels					1759:1766	the florfenicol composite nanogels	1733:1766	the florfenicol composite nanogels	1733:1766	Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver.					
36174982	11	72	theme	composite	1749:1757	arg1	preparation					1793:1803	a biocompatible preparation	1777:1803	a biocompatible preparation	1777:1803	Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver.					
36174982	5	73	theme	zeta	768:771	arg1	potential					773:781	zeta potential	768:781	zeta potential	768:781	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	12	74	theme	SCV	1945:1947	arg1	infections					1949:1958	SCV infections	1945:1958	SCV infections	1945:1958	CONCLUSIONS Florfenicol composite nanogels might improve the treatment of SCV infections.					
36174982	1	75	theme	small	189:193	arg1	SCVs					212:215	SCVs	212:215	SCVs	212:215	BACKGROUND Florfenicol might be ineffective for treating Staphylococcus aureus small colony variants (SCVs) mastitis.					
36174982	1	75	theme	small	189:193	arg1	variants					202:209	Staphylococcus aureus small colony variants	167:209	Staphylococcus aureus small colony variants (SCVs) mastitis	167:225	BACKGROUND Florfenicol might be ineffective for treating Staphylococcus aureus small colony variants (SCVs) mastitis.					
36174982	7	76	dep	in	1109:1110	arg1	vitro					1112:1116	vitro	1112:1116	vitro	1112:1116	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	12	77	theme	CONCLUSIONS	1871:1881	arg1	nanogels					1905:1912	CONCLUSIONS Florfenicol composite nanogels	1871:1912	CONCLUSIONS Florfenicol composite nanogels	1871:1912	CONCLUSIONS Florfenicol composite nanogels might improve the treatment of SCV infections.					
36174982	6	78	link	cross-linked	1072:1083	arg1	networks					1095:1102	cross-linked polymeric networks	1072:1102	cross-linked polymeric networks	1072:1102	Optical and scanning electron microscopy showed that spherical particles with a relatively uniform distribution and drugs might be incorporated in cross-linked polymeric networks.					
36174982	9	79	theme	live-dead	1482:1490	arg1	staining					1502:1509	live-dead bacterial staining	1482:1509	live-dead bacterial staining	1482:1509	The time-killing curves and live-dead bacterial staining showed that the florfenicol composite nanogels were concentration-dependent.					
36174982	5	80	theme	polydispersity	742:755	arg1	index					757:761	polydispersity index	742:761	polydispersity index	742:761	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	4	81	dep	RESULTS	586:592	arg1	obtained					624:631	obtained	624:631	was obtained when the CS and TPP were 10 and 5 mg/mL, respectively	620:685	RESULTS The optimized formulation was obtained when the CS and TPP were 10 and 5 mg/mL, respectively.					
36174982	5	82	theme	±	842:842	arg1	%					847:847	87.3% ± 2.7%	836:847	87.3% ± 2.7%	836:847	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	5	82	theme	±	842:842	arg1	efficiency					706:715	The encapsulation efficiency	688:715	The encapsulation efficiency	688:715	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	5	83	theme	±	901:901	arg1	mv					907:908	36.3 ± 1.4 mv	896:908	36.3 ± 1.4 mv	896:908	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	5	84	theme	composite	812:820	arg1	nanogels					822:829	the optimized florfenicol composite nanogels	786:829	the optimized florfenicol composite nanogels	786:829	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	5	85	theme	0.15	879:882	arg1	±					884:884	0.15 ± 0.03	879:889	0.15 ± 0.03	879:889	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	7	86	theme	%	1249:1249	arg1	release					1227:1233	the sustained release	1213:1233	the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline	1213:1293	The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline.					
36174982	10	87	theme	composite	1617:1625	arg1	nanogels					1627:1634	the florfenicol composite nanogels	1601:1634	the florfenicol composite nanogels	1601:1634	Furthermore, the florfenicol composite nanogels displayed good therapeutic efficacy against SCVs mastitis.					
36174982	0	88	theme	florfenicol	26:36	arg1	composite					38:46	florfenicol composite	26:46	florfenicol composite	26:46	Antibacterial activity of florfenicol composite nanogels against Staphylococcus aureus small colony variants.					
36174982	5	89	theme	nanogels	822:829	arg1	capacity					726:733	loading capacity	718:733	loading capacity	718:733	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	5	89	theme	nanogels	822:829	arg1	%					847:847	87.3% ± 2.7%	836:847	87.3% ± 2.7%	836:847	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	5	89	theme	nanogels	822:829	arg1	potential					773:781	zeta potential	768:781	zeta potential	768:781	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	5	89	theme	nanogels	822:829	arg1	size					736:739	size	736:739	size	736:739	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	5	89	theme	nanogels	822:829	arg1	efficiency					706:715	The encapsulation efficiency	688:715	The encapsulation efficiency	688:715	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	5	89	theme	nanogels	822:829	arg1	index					757:761	polydispersity index	742:761	polydispersity index	742:761	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	12	90	theme	Florfenicol	1883:1893	arg1	nanogels					1905:1912	CONCLUSIONS Florfenicol composite nanogels	1871:1912	CONCLUSIONS Florfenicol composite nanogels	1871:1912	CONCLUSIONS Florfenicol composite nanogels might improve the treatment of SCV infections.					
36174982	9	91	theme	florfenicol	1527:1537	arg1	concentration-dependent					1563:1585	concentration-dependent	1563:1585	concentration-dependent	1563:1585	The time-killing curves and live-dead bacterial staining showed that the florfenicol composite nanogels were concentration-dependent.					
36174982	9	91	theme	florfenicol	1527:1537	arg1	nanogels					1549:1556	the florfenicol composite nanogels	1523:1556	the florfenicol composite nanogels	1523:1556	The time-killing curves and live-dead bacterial staining showed that the florfenicol composite nanogels were concentration-dependent.					
36174982	6	92	theme	spherical	978:986	arg1	particles					988:996	spherical particles	978:996	spherical particles with a relatively uniform distribution and drugs	978:1045	Optical and scanning electron microscopy showed that spherical particles with a relatively uniform distribution and drugs might be incorporated in cross-linked polymeric networks.					
36174982	11	93	theme	Biological	1695:1704	arg1	studies					1713:1719	Biological safety studies	1695:1719	Biological safety studies	1695:1719	Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver.					
36174982	6	94	with	particles	988:996	arg1	drugs					1041:1045	drugs	1041:1045	drugs	1041:1045	Optical and scanning electron microscopy showed that spherical particles with a relatively uniform distribution and drugs might be incorporated in cross-linked polymeric networks.					
36174982	6	94	with	particles	988:996	arg1	distribution					1024:1035	a relatively uniform distribution	1003:1035	a relatively uniform distribution	1003:1035	Optical and scanning electron microscopy showed that spherical particles with a relatively uniform distribution and drugs might be incorporated in cross-linked polymeric networks.					
36174982	10	95	theme	good	1646:1649	arg1	efficacy					1663:1670	good therapeutic efficacy	1646:1670	good therapeutic efficacy against SCVs mastitis	1646:1692	Furthermore, the florfenicol composite nanogels displayed good therapeutic efficacy against SCVs mastitis.					
36174982	8	96	theme	inhibitory	1308:1317	arg1	µg/mL					1433:1437	1 and 0.25 µg/mL	1422:1437	1 and 0.25 µg/mL	1422:1437	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 µg/mL, respectively.					
36174982	8	96	theme	inhibitory	1308:1317	arg1	concentrations					1319:1332	The minimal inhibitory concentrations	1296:1332	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs	1296:1415	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 µg/mL, respectively.					
36174982	10	97	theme	therapeutic	1651:1661	arg1	efficacy					1663:1670	good therapeutic efficacy	1646:1670	good therapeutic efficacy against SCVs mastitis	1646:1692	Furthermore, the florfenicol composite nanogels displayed good therapeutic efficacy against SCVs mastitis.					
36174982	5	98	theme	optimized	790:798	arg1	nanogels					822:829	the optimized florfenicol composite nanogels	786:829	the optimized florfenicol composite nanogels	786:829	The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively.					
36174982	3	99	dep	in	456:457	arg1	vitro					459:463	vitro	459:463	vitro	459:463	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	1	100	theme	aureus	182:187	arg1	SCVs					212:215	SCVs	212:215	SCVs	212:215	BACKGROUND Florfenicol might be ineffective for treating Staphylococcus aureus small colony variants (SCVs) mastitis.					
36174982	1	100	theme	aureus	182:187	arg1	variants					202:209	Staphylococcus aureus small colony variants	167:209	Staphylococcus aureus small colony variants (SCVs) mastitis	167:225	BACKGROUND Florfenicol might be ineffective for treating Staphylococcus aureus small colony variants (SCVs) mastitis.					
36174982	8	101	theme	florfenicol	1348:1358	arg1	solution					1360:1367	commercial florfenicol solution and florfenicol composite nanogels	1337:1402	solution	1360:1367	The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 µg/mL, respectively.					
36174982	3	102	theme	therapeutic	498:508	arg1	efficacy					510:517	therapeutic efficacy	498:517	therapeutic efficacy	498:517	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	3	102	theme	therapeutic	498:508	arg1	METHODS					400:406	METHODS	400:406	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels	400:570	METHODS The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied.					
36174982	2	103	theme	SCV	380:382	arg1	sites					393:397	SCV infected sites	380:397	SCV infected sites	380:397	OBJECTIVES In this study, florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels were prepared to allow targeted delivery to SCV infected sites.					
35240714	5	0	theme	further	644:650	arg1	investigations					668:681	further pharmacological investigations	644:681	further pharmacological investigations	644:681	Prior to further pharmacological investigations, the extracts (100 - 6.25 µg/mL) and compounds 1: - 12: (50 - 5 µM) were tested for their influence on viability of a murine macrophage cell line (J774A.1).					
35240714	8	1	theme	minor	1376:1380	arg1	samaras					1382:1388	U. minor samaras	1373:1388	U. minor samaras supporting their possible alimentary use	1373:1429	These data highlight the anti-inflammatory properties of several isolated compounds from U. minor samaras supporting their possible alimentary use.					
35240714	3	2	gly	glycosylated	452:463	arg1	flavonoids					465:474	two glycosylated flavonoids	448:474	two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature	448:536	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	3	2	gly	glycosylated	452:463	arg1	- 7					480:482	- 7	480:482	- 7	480:482	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	5	3	theme	pharmacological	652:666	arg1	investigations					668:681	further pharmacological investigations	644:681	further pharmacological investigations	644:681	Prior to further pharmacological investigations, the extracts (100 - 6.25 µg/mL) and compounds 1: - 12: (50 - 5 µM) were tested for their influence on viability of a murine macrophage cell line (J774A.1).					
35240714	8	4	theme	compounds	1358:1366	arg1	properties					1327:1336	the anti-inflammatory properties	1305:1336	the anti-inflammatory properties of several isolated compounds from U. minor samaras supporting their possible alimentary use	1305:1429	These data highlight the anti-inflammatory properties of several isolated compounds from U. minor samaras supporting their possible alimentary use.					
35240714	6	5	theme	inhibitory	931:940	arg1	effect					942:947	their inhibitory effect	925:947	their inhibitory effect on some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS)	925:1059	Subsequently, extracts and compounds that did not impede viability, were studied for their inhibitory effect on some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS).					
35240714	7	6	from	expression	1257:1266	arg1	macrophages					1271:1281	macrophages	1271:1281	macrophages	1271:1281	The NO release and the expression of iNOS and COX-2 were then evaluated and both extracts (50 - 6.25 µg/mL) and compounds (20 - 5 µM) significantly inhibited NO release as well as iNOS and COX-2 expression in macrophages.					
35240714	8	7	theme	isolated	1349:1356	arg1	compounds					1358:1366	several isolated compounds	1341:1366	several isolated compounds	1341:1366	These data highlight the anti-inflammatory properties of several isolated compounds from U. minor samaras supporting their possible alimentary use.					
35240714	5	8	theme	line	824:827	arg1	viability					786:794	viability	786:794	viability of a murine macrophage cell line (J774A.1)	786:837	Prior to further pharmacological investigations, the extracts (100 - 6.25 µg/mL) and compounds 1: - 12: (50 - 5 µM) were tested for their influence on viability of a murine macrophage cell line (J774A.1).					
35240714	5	9	theme	cell	819:822	arg1	J774A.1					830:836	J774A.1	830:836	J774A.1	830:836	Prior to further pharmacological investigations, the extracts (100 - 6.25 µg/mL) and compounds 1: - 12: (50 - 5 µM) were tested for their influence on viability of a murine macrophage cell line (J774A.1).					
35240714	5	9	theme	cell	819:822	arg1	line					824:827	murine macrophage cell line	801:827	a murine macrophage cell line (J774A.1)	799:837	Prior to further pharmacological investigations, the extracts (100 - 6.25 µg/mL) and compounds 1: - 12: (50 - 5 µM) were tested for their influence on viability of a murine macrophage cell line (J774A.1).					
35240714	8	10	from	samaras	1382:1388	arg1	properties					1327:1336	the anti-inflammatory properties	1305:1336	the anti-inflammatory properties of several isolated compounds from U. minor samaras supporting their possible alimentary use	1305:1429	These data highlight the anti-inflammatory properties of several isolated compounds from U. minor samaras supporting their possible alimentary use.					
35240714	3	11	from	literature	527:536	arg1	available					510:518	available	510:518	available	510:518	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	8	12	theme	U.	1373:1374	arg1	samaras					1382:1388	U. minor samaras	1373:1388	U. minor samaras supporting their possible alimentary use	1373:1429	These data highlight the anti-inflammatory properties of several isolated compounds from U. minor samaras supporting their possible alimentary use.					
35240714	3	13	theme	n-BuOH	229:234	arg1	extracts					236:243	n-BuOH extracts	229:243	n-BuOH extracts	229:243	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	3	14	dep	acid	432:435	arg1	2					438:438	2	438:438	2	438:438	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	7	15	theme	COX-2	1108:1112	arg1	release					1069:1075	The NO release	1062:1075	The NO release	1062:1075	The NO release and the expression of iNOS and COX-2 were then evaluated and both extracts (50 - 6.25 µg/mL) and compounds (20 - 5 µM) significantly inhibited NO release as well as iNOS and COX-2 expression in macrophages.					
35240714	7	15	theme	COX-2	1108:1112	arg1	expression					1085:1094	the expression	1081:1094	the expression of iNOS and COX-2	1081:1112	The NO release and the expression of iNOS and COX-2 were then evaluated and both extracts (50 - 6.25 µg/mL) and compounds (20 - 5 µM) significantly inhibited NO release as well as iNOS and COX-2 expression in macrophages.					
35240714	2	16	theme	phytochemical	155:167	arg1	investigation					169:181	The phytochemical investigation	151:181	The phytochemical investigation of Ulmus minor subsp	151:202	The phytochemical investigation of Ulmus minor subsp.					
35240714	3	17	theme	compounds	348:356	arg1	characterization					321:336	characterization	321:336	characterization	321:336	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	3	17	theme	compounds	348:356	arg1	isolation					307:315	isolation	307:315	isolation	307:315	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	0	18	theme	subsp	37:41	arg1	Constituents					9:20	Chemical Constituents	0:20	Chemical Constituents of Ulmus minor subsp	0:41	Chemical Constituents of Ulmus minor subsp.					
35240714	3	19	theme	samaras	211:217	arg1	EtOAc					219:223	minor samaras EtOAc	205:223	minor samaras EtOAc	205:223	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	3	20	theme	glycosylated	452:463	arg1	flavonoids					465:474	two glycosylated flavonoids	448:474	two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature	448:536	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	3	20	theme	glycosylated	452:463	arg1	- 7					480:482	- 7	480:482	- 7	480:482	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	3	21	from	available	510:518	arg1	literature					527:536	the literature	523:536	the literature	523:536	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	7	22	theme	NO	1066:1067	arg1	release					1069:1075	The NO release	1062:1075	The NO release	1062:1075	The NO release and the expression of iNOS and COX-2 were then evaluated and both extracts (50 - 6.25 µg/mL) and compounds (20 - 5 µM) significantly inhibited NO release as well as iNOS and COX-2 expression in macrophages.					
35240714	4	23	theme	HRESIMS	621:627	arg1	data					629:632	NMR and HRESIMS data	613:632	NMR and HRESIMS data	613:632	Structure elucidation of the isolated compounds was obtained by 1D and 2D NMR and HRESIMS data.					
35240714	4	24	theme	isolated	568:575	arg1	compounds					577:585	the isolated compounds	564:585	the isolated compounds	564:585	Structure elucidation of the isolated compounds was obtained by 1D and 2D NMR and HRESIMS data.					
35240714	0	25	theme	Chemical	0:7	arg1	Constituents					9:20	Chemical Constituents	0:20	Chemical Constituents of Ulmus minor subsp	0:41	Chemical Constituents of Ulmus minor subsp.					
35240714	3	26	dep	flavan-3-ol	388:398	arg1	1					401:401	1	401:401	1	401:401	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	5	27	from	influence	773:781	arg1	viability					786:794	viability	786:794	viability of a murine macrophage cell line (J774A.1)	786:837	Prior to further pharmacological investigations, the extracts (100 - 6.25 µg/mL) and compounds 1: - 12: (50 - 5 µM) were tested for their influence on viability of a murine macrophage cell line (J774A.1).					
35240714	5	28	theme	murine	801:806	arg1	J774A.1					830:836	J774A.1	830:836	J774A.1	830:836	Prior to further pharmacological investigations, the extracts (100 - 6.25 µg/mL) and compounds 1: - 12: (50 - 5 µM) were tested for their influence on viability of a murine macrophage cell line (J774A.1).					
35240714	5	28	theme	murine	801:806	arg1	line					824:827	murine macrophage cell line	801:827	a murine macrophage cell line (J774A.1)	799:837	Prior to further pharmacological investigations, the extracts (100 - 6.25 µg/mL) and compounds 1: - 12: (50 - 5 µM) were tested for their influence on viability of a murine macrophage cell line (J774A.1).					
35240714	6	29	from	cells	994:998	arg1	mediators					957:965	some mediators	952:965	some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS)	952:1059	Subsequently, extracts and compounds that did not impede viability, were studied for their inhibitory effect on some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS).					
35240714	0	30	theme	Ulmus	25:29	arg1	subsp					37:41	Ulmus minor subsp	25:41	Ulmus minor subsp	25:41	Chemical Constituents of Ulmus minor subsp.					
35240714	3	31	theme	first	278:282	arg1	time					284:287	the first time	274:287	the first time	274:287	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	3	32	theme	minor	205:209	arg1	EtOAc					219:223	minor samaras EtOAc	205:223	minor samaras EtOAc	205:223	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	5	33	theme	macrophage	808:817	arg1	J774A.1					830:836	J774A.1	830:836	J774A.1	830:836	Prior to further pharmacological investigations, the extracts (100 - 6.25 µg/mL) and compounds 1: - 12: (50 - 5 µM) were tested for their influence on viability of a murine macrophage cell line (J774A.1).					
35240714	5	33	theme	macrophage	808:817	arg1	line					824:827	murine macrophage cell line	801:827	a murine macrophage cell line (J774A.1)	799:837	Prior to further pharmacological investigations, the extracts (100 - 6.25 µg/mL) and compounds 1: - 12: (50 - 5 µM) were tested for their influence on viability of a murine macrophage cell line (J774A.1).					
35240714	7	34	theme	NO	1220:1221	arg1	release					1223:1229	NO release	1220:1229	NO release as well as iNOS and COX-2 expression in macrophages	1220:1281	The NO release and the expression of iNOS and COX-2 were then evaluated and both extracts (50 - 6.25 µg/mL) and compounds (20 - 5 µM) significantly inhibited NO release as well as iNOS and COX-2 expression in macrophages.					
35240714	3	35	theme	new	411:413	arg1	acid					432:435	one new trihydroxy fatty acid	407:435	one new trihydroxy fatty acid (2: )	407:441	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	7	36	theme	iNOS	1242:1245	arg1	expression					1257:1266	iNOS and COX-2 expression	1242:1266	NO release as well as iNOS and COX-2 expression in macrophages	1220:1281	The NO release and the expression of iNOS and COX-2 were then evaluated and both extracts (50 - 6.25 µg/mL) and compounds (20 - 5 µM) significantly inhibited NO release as well as iNOS and COX-2 expression in macrophages.					
35240714	8	37	theme	alimentary	1416:1425	arg1	use					1427:1429	their possible alimentary use	1401:1429	their possible alimentary use	1401:1429	These data highlight the anti-inflammatory properties of several isolated compounds from U. minor samaras supporting their possible alimentary use.					
35240714	2	38	theme	subsp	198:202	arg1	investigation					169:181	The phytochemical investigation	151:181	The phytochemical investigation of Ulmus minor subsp	151:202	The phytochemical investigation of Ulmus minor subsp.					
35240714	4	39	dep	1D	603:604	arg1	data					629:632	NMR and HRESIMS data	613:632	NMR and HRESIMS data	613:632	Structure elucidation of the isolated compounds was obtained by 1D and 2D NMR and HRESIMS data.					
35240714	3	40	theme	trihydroxy	415:424	arg1	acid					432:435	one new trihydroxy fatty acid	407:435	one new trihydroxy fatty acid (2: )	407:441	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	3	41	theme	new	384:386	arg1	flavan-3-ol					388:398	one new flavan-3-ol	380:398	one new flavan-3-ol (1: )	380:404	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	6	42	theme	inflammation	970:981	arg1	mediators					957:965	some mediators	952:965	some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS)	952:1059	Subsequently, extracts and compounds that did not impede viability, were studied for their inhibitory effect on some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS).					
35240714	2	43	theme	minor	192:196	arg1	subsp					198:202	Ulmus minor subsp	186:202	Ulmus minor subsp	186:202	The phytochemical investigation of Ulmus minor subsp.					
35240714	3	44	theme	NMR	493:495	arg1	data					497:500	data	497:500	data	497:500	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	7	45	from	release	1223:1229	arg1	macrophages					1271:1281	macrophages	1271:1281	macrophages	1271:1281	The NO release and the expression of iNOS and COX-2 were then evaluated and both extracts (50 - 6.25 µg/mL) and compounds (20 - 5 µM) significantly inhibited NO release as well as iNOS and COX-2 expression in macrophages.					
35240714	4	46	theme	Structure	539:547	arg1	elucidation					549:559	Structure elucidation	539:559	Structure elucidation of the isolated compounds	539:585	Structure elucidation of the isolated compounds was obtained by 1D and 2D NMR and HRESIMS data.					
35240714	0	47	theme	minor	31:35	arg1	subsp					37:41	Ulmus minor subsp	25:41	Ulmus minor subsp	25:41	Chemical Constituents of Ulmus minor subsp.					
35240714	2	48	theme	Ulmus	186:190	arg1	subsp					198:202	Ulmus minor subsp	186:202	Ulmus minor subsp	186:202	The phytochemical investigation of Ulmus minor subsp.					
35240714	6	49	from	mediators	957:965	arg1	cells					994:998	J774A.1 cells	986:998	J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS)	986:1059	Subsequently, extracts and compounds that did not impede viability, were studied for their inhibitory effect on some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS).					
35240714	8	50	theme	several	1341:1347	arg1	compounds					1358:1366	several isolated compounds	1341:1366	several isolated compounds	1341:1366	These data highlight the anti-inflammatory properties of several isolated compounds from U. minor samaras supporting their possible alimentary use.					
35240714	8	51	theme	possible	1407:1414	arg1	use					1427:1429	their possible alimentary use	1401:1429	their possible alimentary use	1401:1429	These data highlight the anti-inflammatory properties of several isolated compounds from U. minor samaras supporting their possible alimentary use.					
35240714	1	52	theme	Anti-inflammatory	112:128	arg1	Activity					130:137	Their Anti-inflammatory Activity	106:137	Their Anti-inflammatory Activity	106:137	minor Fruits Used in the Italian Phytoalimurgic Tradition and Their Anti-inflammatory Activity Evaluation.					
35240714	6	53	from	inflammation	970:981	arg1	cells					994:998	J774A.1 cells	986:998	J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS)	986:1059	Subsequently, extracts and compounds that did not impede viability, were studied for their inhibitory effect on some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS).					
35240714	3	54	theme	fatty	426:430	arg1	acid					432:435	one new trihydroxy fatty acid	407:435	one new trihydroxy fatty acid (2: )	407:441	minor samaras EtOAc and n-BuOH extracts is reported in this work for the first time, resulting in the isolation and characterization of twenty compounds (1: - 20: ) including one new flavan-3-ol (1: ), one new trihydroxy fatty acid (2: ), and two glycosylated flavonoids (6: - 7: ) whose NMR data are not available in the literature.					
35240714	7	55	theme	COX-2	1251:1255	arg1	expression					1257:1266	iNOS and COX-2 expression	1242:1266	NO release as well as iNOS and COX-2 expression in macrophages	1220:1281	The NO release and the expression of iNOS and COX-2 were then evaluated and both extracts (50 - 6.25 µg/mL) and compounds (20 - 5 µM) significantly inhibited NO release as well as iNOS and COX-2 expression in macrophages.					
35240714	6	56	theme	coli	1050:1053	arg1	LPS					1056:1058	LPS	1056:1058	LPS	1056:1058	Subsequently, extracts and compounds that did not impede viability, were studied for their inhibitory effect on some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS).					
35240714	6	56	theme	coli	1050:1053	arg1	lipopolysaccharide					1016:1033	lipopolysaccharide	1016:1033	lipopolysaccharide of Escherichia coli (LPS)	1016:1059	Subsequently, extracts and compounds that did not impede viability, were studied for their inhibitory effect on some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS).					
35240714	6	57	from	effect	942:947	arg1	mediators					957:965	some mediators	952:965	some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS)	952:1059	Subsequently, extracts and compounds that did not impede viability, were studied for their inhibitory effect on some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS).					
35240714	7	58	theme	iNOS	1099:1102	arg1	release					1069:1075	The NO release	1062:1075	The NO release	1062:1075	The NO release and the expression of iNOS and COX-2 were then evaluated and both extracts (50 - 6.25 µg/mL) and compounds (20 - 5 µM) significantly inhibited NO release as well as iNOS and COX-2 expression in macrophages.					
35240714	7	58	theme	iNOS	1099:1102	arg1	expression					1085:1094	the expression	1081:1094	the expression of iNOS and COX-2	1081:1112	The NO release and the expression of iNOS and COX-2 were then evaluated and both extracts (50 - 6.25 µg/mL) and compounds (20 - 5 µM) significantly inhibited NO release as well as iNOS and COX-2 expression in macrophages.					
35240714	4	59	theme	NMR	613:615	arg1	data					629:632	NMR and HRESIMS data	613:632	NMR and HRESIMS data	613:632	Structure elucidation of the isolated compounds was obtained by 1D and 2D NMR and HRESIMS data.					
35240714	8	60	theme	anti-inflammatory	1309:1325	arg1	properties					1327:1336	the anti-inflammatory properties	1305:1336	the anti-inflammatory properties of several isolated compounds from U. minor samaras supporting their possible alimentary use	1305:1429	These data highlight the anti-inflammatory properties of several isolated compounds from U. minor samaras supporting their possible alimentary use.					
35240714	5	61	dep	compounds	720:728	arg1	- 12					733:736	- 12	733:736	- 12	733:736	Prior to further pharmacological investigations, the extracts (100 - 6.25 µg/mL) and compounds 1: - 12: (50 - 5 µM) were tested for their influence on viability of a murine macrophage cell line (J774A.1).					
35240714	5	61	dep	compounds	720:728	arg1	1					730:730	1	730:730	1	730:730	Prior to further pharmacological investigations, the extracts (100 - 6.25 µg/mL) and compounds 1: - 12: (50 - 5 µM) were tested for their influence on viability of a murine macrophage cell line (J774A.1).					
35240714	1	62	dep	Tradition	92:100	arg1	the					65:67	the	65:67	the	65:67	minor Fruits Used in the Italian Phytoalimurgic Tradition and Their Anti-inflammatory Activity Evaluation.					
35240714	1	62	dep	Tradition	92:100	arg1	Evaluation					139:148	Evaluation	139:148	Evaluation	139:148	minor Fruits Used in the Italian Phytoalimurgic Tradition and Their Anti-inflammatory Activity Evaluation.					
35240714	6	63	theme	J774A.1	986:992	arg1	cells					994:998	J774A.1 cells	986:998	J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS)	986:1059	Subsequently, extracts and compounds that did not impede viability, were studied for their inhibitory effect on some mediators of inflammation in J774A.1 cells stimulated with lipopolysaccharide of Escherichia coli (LPS).					
35240714	1	64	theme	minor	44:48	arg1	Fruits					50:55	minor Fruits	44:55	minor Fruits	44:55	minor Fruits Used in the Italian Phytoalimurgic Tradition and Their Anti-inflammatory Activity Evaluation.					
35240714	4	65	theme	compounds	577:585	arg1	elucidation					549:559	Structure elucidation	539:559	Structure elucidation of the isolated compounds	539:585	Structure elucidation of the isolated compounds was obtained by 1D and 2D NMR and HRESIMS data.					
35537603	8	0	theme	human	1167:1171	arg1	model					1185:1189	model	1185:1189	model	1185:1189	The anti-inflammatory activity was tested using the LPS-stimulated human neutrophils model and ELISA test.					
35537603	8	0	theme	human	1167:1171	arg1	test					1201:1204	ELISA test	1195:1204	ELISA test	1195:1204	The anti-inflammatory activity was tested using the LPS-stimulated human neutrophils model and ELISA test.					
35537603	8	0	theme	human	1167:1171	arg1	neutrophils					1173:1183	the LPS-stimulated human neutrophils	1148:1183	the LPS-stimulated human neutrophils model and ELISA test	1148:1204	The anti-inflammatory activity was tested using the LPS-stimulated human neutrophils model and ELISA test.					
35537603	6	1	theme	UHPLC-DAD-MSn	887:899	arg1	analysis					901:908	The UHPLC-DAD-MSn analysis	883:908	The UHPLC-DAD-MSn analysis	883:908	The UHPLC-DAD-MSn analysis determined the composition of raw extract and analysis of microbial metabolites.					
35537603	13	2	theme	anti-inflammatory	1868:1884	arg1	effect					1886:1891	The anti-inflammatory effect	1864:1891	The anti-inflammatory effect	1864:1891	The anti-inflammatory effect might be linked to both microbiota composition changes and direct activity of bioavailable metabolites.					
35537603	11	3	theme	production	1659:1668	arg1	reduction					1640:1648	the reduction	1636:1648	the reduction of TNF-α production	1636:1668	Raw extract and their metabolites exhibit different levels of inhibition of cytokines production by LPS-stimulated neutrophils, but the reduction of TNF-α production was observed.					
35537603	4	4	dep	aimed	539:543	arg1	AIM					512:514	AIM	512:514	AIM OF THE STUDY	512:527	AIM OF THE STUDY The study aimed to determine the interaction between human gut microbiota and the linden flower extracts, resulting in the biotransformation of the extract's constituents and changes in the microbiota composition.					
35537603	1	5	theme	cold	236:239	arg1	disturbances					289:300	the common cold, throat irritation, and upper respiratory tract disturbances	225:300	the common cold, throat irritation, and upper respiratory tract disturbances	225:300	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	7	6	from	changes	1065:1071	arg1	composition					1087:1097	microbiota composition	1076:1097	microbiota composition	1076:1097	The intestinal microbiota isolation and sequencing were used to determine changes in microbiota composition.					
35537603	15	7	theme	compounds	2126:2134	arg1	concentration					2100:2112	A low concentration	2094:2112	A low concentration of phenolic compounds in metabolized linden flower extract	2094:2171	A low concentration of phenolic compounds in metabolized linden flower extract and responsible for anti-inflammatory properties, and the multitude of biological and chemical particles and their interactions may weaken these properties.					
35537603	10	8	theme	microbiota	1404:1413	arg1	composition					1415:1425	human gut microbiota composition	1394:1425	human gut microbiota composition	1394:1425	The extract changed human gut microbiota composition but did not cause dysbiosis (change in the abundance of forty-three genera).					
35537603	1	9	theme	throat	242:247	arg1	irritation					249:258	throat irritation	242:258	throat irritation	242:258	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	8	10	theme	ELISA	1195:1199	arg1	test					1201:1204	ELISA test	1195:1204	ELISA test	1195:1204	The anti-inflammatory activity was tested using the LPS-stimulated human neutrophils model and ELISA test.					
35537603	8	10	theme	ELISA	1195:1199	arg1	neutrophils					1173:1183	the LPS-stimulated human neutrophils	1148:1183	the LPS-stimulated human neutrophils model and ELISA test	1148:1204	The anti-inflammatory activity was tested using the LPS-stimulated human neutrophils model and ELISA test.					
35537603	11	11	theme	production	1590:1599	arg1	inhibition					1566:1575	inhibition	1566:1575	inhibition of cytokines production by LPS-stimulated neutrophils	1566:1629	Raw extract and their metabolites exhibit different levels of inhibition of cytokines production by LPS-stimulated neutrophils, but the reduction of TNF-α production was observed.					
35537603	5	12	theme	human	838:842	arg1	slurries					851:858	human faecal slurries	838:858	human faecal slurries from 5 healthy donors	838:880	MATERIAL AND METHODS The linden flower metabolites were obtained by incubation of extract with human faecal slurries from 5 healthy donors.					
35537603	9	13	theme	linden	1235:1240	arg1	extract					1249:1255	linden flower extract	1235:1255	linden flower extract	1235:1255	RESULTS After incubation of linden flower extract with human gut microbiota, twenty metabolites were detected and characterized, and three among them were identified.					
35537603	15	14	theme	particles	2268:2276	arg1	multitude					2231:2239	the multitude	2227:2239	the multitude of biological and chemical particles and their interactions	2227:2299	A low concentration of phenolic compounds in metabolized linden flower extract and responsible for anti-inflammatory properties, and the multitude of biological and chemical particles and their interactions may weaken these properties.					
35537603	9	15	theme	extract	1249:1255	arg1	incubation					1221:1230	incubation	1221:1230	incubation of linden flower extract with human gut microbiota	1221:1281	RESULTS After incubation of linden flower extract with human gut microbiota, twenty metabolites were detected and characterized, and three among them were identified.					
35537603	6	16	theme	raw	940:942	arg1	extract					944:950	raw extract	940:950	raw extract	940:950	The UHPLC-DAD-MSn analysis determined the composition of raw extract and analysis of microbial metabolites.					
35537603	4	17	theme	linden	611:616	arg1	extracts					625:632	the linden flower extracts	607:632	the linden flower extracts	607:632	AIM OF THE STUDY The study aimed to determine the interaction between human gut microbiota and the linden flower extracts, resulting in the biotransformation of the extract's constituents and changes in the microbiota composition.					
35537603	9	18	theme	human	1262:1266	arg1	microbiota					1272:1281	human gut microbiota	1262:1281	human gut microbiota	1262:1281	RESULTS After incubation of linden flower extract with human gut microbiota, twenty metabolites were detected and characterized, and three among them were identified.					
35537603	5	19	theme	extract	825:831	arg1	incubation					811:820	incubation	811:820	incubation of extract with human faecal slurries from 5 healthy donors	811:880	MATERIAL AND METHODS The linden flower metabolites were obtained by incubation of extract with human faecal slurries from 5 healthy donors.					
35537603	7	20	used	used	1047:1050	arg2	microbiota					1006:1015	The intestinal microbiota isolation and sequencing	991:1040	The intestinal microbiota isolation and sequencing	991:1040	The intestinal microbiota isolation and sequencing were used to determine changes in microbiota composition.					
35537603	4	21	theme	constituents	687:698	arg1	biotransformation					652:668	the biotransformation	648:668	the biotransformation of the extract's constituents	648:698	AIM OF THE STUDY The study aimed to determine the interaction between human gut microbiota and the linden flower extracts, resulting in the biotransformation of the extract's constituents and changes in the microbiota composition.					
35537603	4	21	theme	constituents	687:698	arg1	changes					704:710	changes	704:710	changes in the microbiota composition	704:740	AIM OF THE STUDY The study aimed to determine the interaction between human gut microbiota and the linden flower extracts, resulting in the biotransformation of the extract's constituents and changes in the microbiota composition.					
35537603	13	22	theme	bioavailable	1971:1982	arg1	metabolites					1984:1994	bioavailable metabolites	1971:1994	bioavailable metabolites	1971:1994	The anti-inflammatory effect might be linked to both microbiota composition changes and direct activity of bioavailable metabolites.					
35537603	12	23	theme	gut	1758:1760	arg1	microbiota					1762:1771	human gut microbiota	1752:1771	human gut microbiota	1752:1771	CONCLUSIONS The linden flower extract has a beneficial influence on human gut microbiota because it promotes increasing the abundance of bacteria responsible for SCFAs production.					
35537603	0	24	theme	human	58:62	arg1	biodiversity					79:90	human gut microbiota biodiversity	58:90	human gut microbiota biodiversity	58:90	Tiliae flos metabolites and their beneficial influence on human gut microbiota biodiversity ex vivo.					
35537603	0	25	from	metabolites	12:22	arg1	biodiversity					79:90	human gut microbiota biodiversity	58:90	human gut microbiota biodiversity	58:90	Tiliae flos metabolites and their beneficial influence on human gut microbiota biodiversity ex vivo.					
35537603	0	26	theme	microbiota	68:77	arg1	biodiversity					79:90	human gut microbiota biodiversity	58:90	human gut microbiota biodiversity	58:90	Tiliae flos metabolites and their beneficial influence on human gut microbiota biodiversity ex vivo.					
35537603	12	27	theme	beneficial	1728:1737	arg1	influence					1739:1747	a beneficial influence	1726:1747	a beneficial influence	1726:1747	CONCLUSIONS The linden flower extract has a beneficial influence on human gut microbiota because it promotes increasing the abundance of bacteria responsible for SCFAs production.					
35537603	6	28	theme	metabolites	978:988	arg1	composition					925:935	the composition	921:935	the composition of raw extract	921:950	The UHPLC-DAD-MSn analysis determined the composition of raw extract and analysis of microbial metabolites.					
35537603	6	28	theme	metabolites	978:988	arg1	analysis					956:963	analysis	956:963	analysis of microbial metabolites	956:988	The UHPLC-DAD-MSn analysis determined the composition of raw extract and analysis of microbial metabolites.					
35537603	11	29	theme	different	1546:1554	arg1	levels					1556:1561	different levels	1546:1561	different levels of inhibition of cytokines production by LPS-stimulated neutrophils	1546:1629	Raw extract and their metabolites exhibit different levels of inhibition of cytokines production by LPS-stimulated neutrophils, but the reduction of TNF-α production was observed.					
35537603	15	30	theme	phenolic	2117:2124	arg1	compounds					2126:2134	phenolic compounds	2117:2134	phenolic compounds	2117:2134	A low concentration of phenolic compounds in metabolized linden flower extract and responsible for anti-inflammatory properties, and the multitude of biological and chemical particles and their interactions may weaken these properties.					
35537603	0	31	from	influence	45:53	arg1	biodiversity					79:90	human gut microbiota biodiversity	58:90	human gut microbiota biodiversity	58:90	Tiliae flos metabolites and their beneficial influence on human gut microbiota biodiversity ex vivo.					
35537603	10	32	theme	genera	1495:1500	arg1	abundance					1470:1478	the abundance	1466:1478	the abundance of forty-three genera	1466:1500	The extract changed human gut microbiota composition but did not cause dysbiosis (change in the abundance of forty-three genera).					
35537603	1	33	dep	RELEVANCE	122:130	arg1	flower					143:148	The linden flower	132:148	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos)	101:162	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	1	33	dep	RELEVANCE	122:130	arg1	flos					158:161	Tiliae flos	151:161	Tiliae flos	151:161	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	3	34	theme	pharmacopeial	412:424	arg1	material					432:439	pharmacopeial plant material	412:439	pharmacopeial plant material	412:439	Although it is pharmacopeial plant material, there are no reports about its interaction with human gut microbiota.					
35537603	13	35	attach	linked	1902:1907	arg2	effect					1886:1891	The anti-inflammatory effect	1864:1891	The anti-inflammatory effect	1864:1891	The anti-inflammatory effect might be linked to both microbiota composition changes and direct activity of bioavailable metabolites.					
35537603	13	35	attach	linked	1902:1907	arg1	changes					1940:1946	microbiota composition changes	1917:1946	microbiota composition changes	1917:1946	The anti-inflammatory effect might be linked to both microbiota composition changes and direct activity of bioavailable metabolites.					
35537603	13	35	attach	linked	1902:1907	arg1	activity					1959:1966	direct activity	1952:1966	direct activity of bioavailable metabolites	1952:1994	The anti-inflammatory effect might be linked to both microbiota composition changes and direct activity of bioavailable metabolites.					
35537603	15	36	theme	linden	2151:2156	arg1	extract					2165:2171	metabolized linden flower extract	2139:2171	metabolized linden flower extract	2139:2171	A low concentration of phenolic compounds in metabolized linden flower extract and responsible for anti-inflammatory properties, and the multitude of biological and chemical particles and their interactions may weaken these properties.					
35537603	12	37	theme	flower	1707:1712	arg1	extract					1714:1720	The linden flower extract	1696:1720	The linden flower extract	1696:1720	CONCLUSIONS The linden flower extract has a beneficial influence on human gut microbiota because it promotes increasing the abundance of bacteria responsible for SCFAs production.					
35537603	13	38	theme	composition	1928:1938	arg1	changes					1940:1946	microbiota composition changes	1917:1946	microbiota composition changes	1917:1946	The anti-inflammatory effect might be linked to both microbiota composition changes and direct activity of bioavailable metabolites.					
35537603	0	39	theme	Tiliae	0:5	arg1	metabolites					12:22	Tiliae flos metabolites	0:22	Tiliae flos metabolites	0:22	Tiliae flos metabolites and their beneficial influence on human gut microbiota biodiversity ex vivo.					
35537603	1	40	theme	irritation	249:258	arg1	disturbances					289:300	the common cold, throat irritation, and upper respiratory tract disturbances	225:300	the common cold, throat irritation, and upper respiratory tract disturbances	225:300	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	4	41	from	changes	704:710	arg1	composition					730:740	the microbiota composition	715:740	the microbiota composition	715:740	AIM OF THE STUDY The study aimed to determine the interaction between human gut microbiota and the linden flower extracts, resulting in the biotransformation of the extract's constituents and changes in the microbiota composition.					
35537603	14	42	theme	pro-inflammatory	2066:2081	arg1	cytokines					2083:2091	pro-inflammatory cytokines	2066:2091	pro-inflammatory cytokines	2066:2091	Increased abundance of SCFAs producers may inhibit the production of pro-inflammatory cytokines.					
35537603	15	43	theme	low	2096:2098	arg1	concentration					2100:2112	A low concentration	2094:2112	A low concentration of phenolic compounds in metabolized linden flower extract	2094:2171	A low concentration of phenolic compounds in metabolized linden flower extract and responsible for anti-inflammatory properties, and the multitude of biological and chemical particles and their interactions may weaken these properties.					
35537603	9	44	with	incubation	1221:1230	arg1	microbiota					1272:1281	human gut microbiota	1262:1281	human gut microbiota	1262:1281	RESULTS After incubation of linden flower extract with human gut microbiota, twenty metabolites were detected and characterized, and three among them were identified.					
35537603	1	45	theme	ETHNOPHARMACOLOGICAL	101:120	arg1	RELEVANCE					122:130	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower	101:148	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos)	101:162	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	1	46	theme	respiratory	271:281	arg1	tract					283:287	upper respiratory tract	265:287	upper respiratory tract	265:287	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	12	47	contain	has	1722:1724	arg1	extract					1714:1720	The linden flower extract	1696:1720	The linden flower extract	1696:1720	CONCLUSIONS The linden flower extract has a beneficial influence on human gut microbiota because it promotes increasing the abundance of bacteria responsible for SCFAs production.					
35537603	12	47	contain	has	1722:1724	arg2	influence					1739:1747	a beneficial influence	1726:1747	a beneficial influence	1726:1747	CONCLUSIONS The linden flower extract has a beneficial influence on human gut microbiota because it promotes increasing the abundance of bacteria responsible for SCFAs production.					
35537603	5	48	theme	linden	768:773	arg1	metabolites					782:792	The linden flower metabolites	764:792	The linden flower metabolites	764:792	MATERIAL AND METHODS The linden flower metabolites were obtained by incubation of extract with human faecal slurries from 5 healthy donors.					
35537603	1	49	theme	disturbances	289:300	arg1	symptoms					213:220	symptoms	213:220	symptoms of the common cold, throat irritation, and upper respiratory tract disturbances	213:300	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	8	50	theme	anti-inflammatory	1104:1120	arg1	activity					1122:1129	The anti-inflammatory activity	1100:1129	The anti-inflammatory activity	1100:1129	The anti-inflammatory activity was tested using the LPS-stimulated human neutrophils model and ELISA test.					
35537603	14	51	theme	producers	2026:2034	arg1	abundance					2007:2015	Increased abundance	1997:2015	Increased abundance of SCFAs producers	1997:2034	Increased abundance of SCFAs producers may inhibit the production of pro-inflammatory cytokines.					
35537603	15	52	theme	anti-inflammatory	2193:2209	arg1	properties					2211:2220	anti-inflammatory properties	2193:2220	anti-inflammatory properties	2193:2220	A low concentration of phenolic compounds in metabolized linden flower extract and responsible for anti-inflammatory properties, and the multitude of biological and chemical particles and their interactions may weaken these properties.					
35537603	12	53	theme	responsible	1830:1840	arg1	bacteria					1821:1828	bacteria	1821:1828	bacteria responsible for SCFAs production	1821:1861	CONCLUSIONS The linden flower extract has a beneficial influence on human gut microbiota because it promotes increasing the abundance of bacteria responsible for SCFAs production.					
35537603	11	54	theme	TNF-α	1653:1657	arg1	production					1659:1668	TNF-α production	1653:1668	TNF-α production	1653:1668	Raw extract and their metabolites exhibit different levels of inhibition of cytokines production by LPS-stimulated neutrophils, but the reduction of TNF-α production was observed.					
35537603	3	55	theme	human	490:494	arg1	microbiota					500:509	human gut microbiota	490:509	human gut microbiota	490:509	Although it is pharmacopeial plant material, there are no reports about its interaction with human gut microbiota.					
35537603	0	56	dep	Tiliae	0:5	arg1	flos					7:10	flos	7:10	flos	7:10	Tiliae flos metabolites and their beneficial influence on human gut microbiota biodiversity ex vivo.					
35537603	12	57	theme	SCFAs	1846:1850	arg1	production					1852:1861	SCFAs production	1846:1861	SCFAs production	1846:1861	CONCLUSIONS The linden flower extract has a beneficial influence on human gut microbiota because it promotes increasing the abundance of bacteria responsible for SCFAs production.					
35537603	4	58	theme	human	582:586	arg1	microbiota					592:601	human gut microbiota	582:601	human gut microbiota	582:601	AIM OF THE STUDY The study aimed to determine the interaction between human gut microbiota and the linden flower extracts, resulting in the biotransformation of the extract's constituents and changes in the microbiota composition.					
35537603	3	59	with	interaction	473:483	arg1	microbiota					500:509	human gut microbiota	490:509	human gut microbiota	490:509	Although it is pharmacopeial plant material, there are no reports about its interaction with human gut microbiota.					
35537603	12	60	dep	CONCLUSIONS	1684:1694	arg1	has					1722:1724	has	1722:1724	has a beneficial influence on human gut microbiota because it promotes increasing the abundance of bacteria responsible for SCFAs production	1722:1861	CONCLUSIONS The linden flower extract has a beneficial influence on human gut microbiota because it promotes increasing the abundance of bacteria responsible for SCFAs production.					
35537603	4	61	from	biotransformation	652:668	arg1	composition					730:740	the microbiota composition	715:740	the microbiota composition	715:740	AIM OF THE STUDY The study aimed to determine the interaction between human gut microbiota and the linden flower extracts, resulting in the biotransformation of the extract's constituents and changes in the microbiota composition.					
35537603	1	62	theme	common	229:234	arg1	cold					236:239	common cold	229:239	common cold	229:239	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	8	63	theme	LPS-stimulated	1152:1165	arg1	model					1185:1189	model	1185:1189	model	1185:1189	The anti-inflammatory activity was tested using the LPS-stimulated human neutrophils model and ELISA test.					
35537603	8	63	theme	LPS-stimulated	1152:1165	arg1	test					1201:1204	ELISA test	1195:1204	ELISA test	1195:1204	The anti-inflammatory activity was tested using the LPS-stimulated human neutrophils model and ELISA test.					
35537603	8	63	theme	LPS-stimulated	1152:1165	arg1	neutrophils					1173:1183	the LPS-stimulated human neutrophils	1148:1183	the LPS-stimulated human neutrophils model and ELISA test	1148:1204	The anti-inflammatory activity was tested using the LPS-stimulated human neutrophils model and ELISA test.					
35537603	11	64	theme	inhibition	1566:1575	arg1	levels					1556:1561	different levels	1546:1561	different levels of inhibition of cytokines production by LPS-stimulated neutrophils	1546:1629	Raw extract and their metabolites exhibit different levels of inhibition of cytokines production by LPS-stimulated neutrophils, but the reduction of TNF-α production was observed.					
35537603	15	65	theme	interactions	2288:2299	arg1	multitude					2231:2239	the multitude	2227:2239	the multitude of biological and chemical particles and their interactions	2227:2299	A low concentration of phenolic compounds in metabolized linden flower extract and responsible for anti-inflammatory properties, and the multitude of biological and chemical particles and their interactions may weaken these properties.					
35537603	10	66	theme	gut	1400:1402	arg1	composition					1415:1425	human gut microbiota composition	1394:1425	human gut microbiota composition	1394:1425	The extract changed human gut microbiota composition but did not cause dysbiosis (change in the abundance of forty-three genera).					
35537603	15	67	theme	chemical	2259:2266	arg1	particles					2268:2276	biological and chemical particles	2244:2276	biological and chemical particles	2244:2276	A low concentration of phenolic compounds in metabolized linden flower extract and responsible for anti-inflammatory properties, and the multitude of biological and chemical particles and their interactions may weaken these properties.					
35537603	4	68	theme	flower	618:623	arg1	extracts					625:632	the linden flower extracts	607:632	the linden flower extracts	607:632	AIM OF THE STUDY The study aimed to determine the interaction between human gut microbiota and the linden flower extracts, resulting in the biotransformation of the extract's constituents and changes in the microbiota composition.					
35537603	7	69	theme	microbiota	1076:1085	arg1	composition					1087:1097	microbiota composition	1076:1097	microbiota composition	1076:1097	The intestinal microbiota isolation and sequencing were used to determine changes in microbiota composition.					
35537603	5	70	theme	faecal	844:849	arg1	slurries					851:858	human faecal slurries	838:858	human faecal slurries from 5 healthy donors	838:880	MATERIAL AND METHODS The linden flower metabolites were obtained by incubation of extract with human faecal slurries from 5 healthy donors.					
35537603	10	71	from	change	1456:1461	arg1	abundance					1470:1478	the abundance	1466:1478	the abundance of forty-three genera	1466:1500	The extract changed human gut microbiota composition but did not cause dysbiosis (change in the abundance of forty-three genera).					
35537603	9	72	theme	flower	1242:1247	arg1	extract					1249:1255	linden flower extract	1235:1255	linden flower extract	1235:1255	RESULTS After incubation of linden flower extract with human gut microbiota, twenty metabolites were detected and characterized, and three among them were identified.					
35537603	15	73	theme	biological	2244:2253	arg1	particles					2268:2276	biological and chemical particles	2244:2276	biological and chemical particles	2244:2276	A low concentration of phenolic compounds in metabolized linden flower extract and responsible for anti-inflammatory properties, and the multitude of biological and chemical particles and their interactions may weaken these properties.					
35537603	6	74	theme	extract	944:950	arg1	composition					925:935	the composition	921:935	the composition of raw extract	921:950	The UHPLC-DAD-MSn analysis determined the composition of raw extract and analysis of microbial metabolites.					
35537603	6	74	theme	extract	944:950	arg1	analysis					956:963	analysis	956:963	analysis of microbial metabolites	956:988	The UHPLC-DAD-MSn analysis determined the composition of raw extract and analysis of microbial metabolites.					
35537603	5	75	dep	MATERIAL	743:750	arg1	metabolites					782:792	The linden flower metabolites	764:792	The linden flower metabolites	764:792	MATERIAL AND METHODS The linden flower metabolites were obtained by incubation of extract with human faecal slurries from 5 healthy donors.					
35537603	9	76	theme	gut	1268:1270	arg1	microbiota					1272:1281	human gut microbiota	1262:1281	human gut microbiota	1262:1281	RESULTS After incubation of linden flower extract with human gut microbiota, twenty metabolites were detected and characterized, and three among them were identified.					
35537603	11	77	theme	Raw	1504:1506	arg1	extract					1508:1514	Raw extract	1504:1514	Raw extract	1504:1514	Raw extract and their metabolites exhibit different levels of inhibition of cytokines production by LPS-stimulated neutrophils, but the reduction of TNF-α production was observed.					
35537603	11	78	theme	cytokines	1580:1588	arg1	production					1590:1599	cytokines production	1580:1599	cytokines production	1580:1599	Raw extract and their metabolites exhibit different levels of inhibition of cytokines production by LPS-stimulated neutrophils, but the reduction of TNF-α production was observed.					
35537603	7	79	theme	intestinal	995:1004	arg1	microbiota					1006:1015	The intestinal microbiota isolation and sequencing	991:1040	The intestinal microbiota isolation and sequencing	991:1040	The intestinal microbiota isolation and sequencing were used to determine changes in microbiota composition.					
35537603	1	80	theme	linden	136:141	arg1	flower					143:148	The linden flower	132:148	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos)	101:162	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	1	81	used	used	173:176	arg2	RELEVANCE					122:130	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower	101:148	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos)	101:162	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	13	82	theme	metabolites	1984:1994	arg1	changes					1940:1946	microbiota composition changes	1917:1946	microbiota composition changes	1917:1946	The anti-inflammatory effect might be linked to both microbiota composition changes and direct activity of bioavailable metabolites.					
35537603	13	82	theme	metabolites	1984:1994	arg1	activity					1959:1966	direct activity	1952:1966	direct activity of bioavailable metabolites	1952:1994	The anti-inflammatory effect might be linked to both microbiota composition changes and direct activity of bioavailable metabolites.					
35537603	0	83	theme	gut	64:66	arg1	biodiversity					79:90	human gut microbiota biodiversity	58:90	human gut microbiota biodiversity	58:90	Tiliae flos metabolites and their beneficial influence on human gut microbiota biodiversity ex vivo.					
35537603	12	84	theme	human	1752:1756	arg1	microbiota					1762:1771	human gut microbiota	1752:1771	human gut microbiota	1752:1771	CONCLUSIONS The linden flower extract has a beneficial influence on human gut microbiota because it promotes increasing the abundance of bacteria responsible for SCFAs production.					
35537603	5	85	theme	healthy	867:873	arg1	donors					875:880	5 healthy donors	865:880	5 healthy donors	865:880	MATERIAL AND METHODS The linden flower metabolites were obtained by incubation of extract with human faecal slurries from 5 healthy donors.					
35537603	13	86	theme	direct	1952:1957	arg1	activity					1959:1966	direct activity	1952:1966	direct activity of bioavailable metabolites	1952:1994	The anti-inflammatory effect might be linked to both microbiota composition changes and direct activity of bioavailable metabolites.					
35537603	5	87	from	donors	875:880	arg1	slurries					851:858	human faecal slurries	838:858	human faecal slurries from 5 healthy donors	838:880	MATERIAL AND METHODS The linden flower metabolites were obtained by incubation of extract with human faecal slurries from 5 healthy donors.					
35537603	15	88	theme	flower	2158:2163	arg1	extract					2165:2171	metabolized linden flower extract	2139:2171	metabolized linden flower extract	2139:2171	A low concentration of phenolic compounds in metabolized linden flower extract and responsible for anti-inflammatory properties, and the multitude of biological and chemical particles and their interactions may weaken these properties.					
35537603	6	89	theme	microbial	968:976	arg1	metabolites					978:988	microbial metabolites	968:988	microbial metabolites	968:988	The UHPLC-DAD-MSn analysis determined the composition of raw extract and analysis of microbial metabolites.					
35537603	8	90	dep	neutrophils	1173:1183	arg1	model					1185:1189	model	1185:1189	model	1185:1189	The anti-inflammatory activity was tested using the LPS-stimulated human neutrophils model and ELISA test.					
35537603	8	90	dep	neutrophils	1173:1183	arg1	test					1201:1204	ELISA test	1195:1204	ELISA test	1195:1204	The anti-inflammatory activity was tested using the LPS-stimulated human neutrophils model and ELISA test.					
35537603	8	90	dep	neutrophils	1173:1183	arg1	neutrophils					1173:1183	the LPS-stimulated human neutrophils	1148:1183	the LPS-stimulated human neutrophils model and ELISA test	1148:1204	The anti-inflammatory activity was tested using the LPS-stimulated human neutrophils model and ELISA test.					
35537603	4	91	theme	microbiota	719:728	arg1	composition					730:740	the microbiota composition	715:740	the microbiota composition	715:740	AIM OF THE STUDY The study aimed to determine the interaction between human gut microbiota and the linden flower extracts, resulting in the biotransformation of the extract's constituents and changes in the microbiota composition.					
35537603	15	92	from	concentration	2100:2112	arg1	extract					2165:2171	metabolized linden flower extract	2139:2171	metabolized linden flower extract	2139:2171	A low concentration of phenolic compounds in metabolized linden flower extract and responsible for anti-inflammatory properties, and the multitude of biological and chemical particles and their interactions may weaken these properties.					
35537603	15	93	theme	metabolized	2139:2149	arg1	extract					2165:2171	metabolized linden flower extract	2139:2171	metabolized linden flower extract	2139:2171	A low concentration of phenolic compounds in metabolized linden flower extract and responsible for anti-inflammatory properties, and the multitude of biological and chemical particles and their interactions may weaken these properties.					
35537603	7	94	dep	microbiota	1006:1015	arg1	sequencing					1031:1040	sequencing	1031:1040	sequencing	1031:1040	The intestinal microbiota isolation and sequencing were used to determine changes in microbiota composition.					
35537603	7	94	dep	microbiota	1006:1015	arg1	isolation					1017:1025	isolation	1017:1025	isolation	1017:1025	The intestinal microbiota isolation and sequencing were used to determine changes in microbiota composition.					
35537603	3	95	theme	plant	426:430	arg1	material					432:439	pharmacopeial plant material	412:439	pharmacopeial plant material	412:439	Although it is pharmacopeial plant material, there are no reports about its interaction with human gut microbiota.					
35537603	5	96	theme	flower	775:780	arg1	metabolites					782:792	The linden flower metabolites	764:792	The linden flower metabolites	764:792	MATERIAL AND METHODS The linden flower metabolites were obtained by incubation of extract with human faecal slurries from 5 healthy donors.					
35537603	14	97	theme	cytokines	2083:2091	arg1	production					2052:2061	the production	2048:2061	the production of pro-inflammatory cytokines	2048:2091	Increased abundance of SCFAs producers may inhibit the production of pro-inflammatory cytokines.					
35537603	12	98	theme	linden	1700:1705	arg1	extract					1714:1720	The linden flower extract	1696:1720	The linden flower extract	1696:1720	CONCLUSIONS The linden flower extract has a beneficial influence on human gut microbiota because it promotes increasing the abundance of bacteria responsible for SCFAs production.					
35537603	4	99	theme	STUDY	523:527	arg1	AIM					512:514	AIM	512:514	AIM OF THE STUDY	512:527	AIM OF THE STUDY The study aimed to determine the interaction between human gut microbiota and the linden flower extracts, resulting in the biotransformation of the extract's constituents and changes in the microbiota composition.					
35537603	0	100	theme	beneficial	34:43	arg1	influence					45:53	their beneficial influence	28:53	their beneficial influence on human gut microbiota biodiversity ex vivo	28:98	Tiliae flos metabolites and their beneficial influence on human gut microbiota biodiversity ex vivo.					
35537603	1	101	theme	upper	265:269	arg1	tract					283:287	upper respiratory tract	265:287	upper respiratory tract	265:287	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	5	102	with	incubation	811:820	arg1	slurries					851:858	human faecal slurries	838:858	human faecal slurries from 5 healthy donors	838:880	MATERIAL AND METHODS The linden flower metabolites were obtained by incubation of extract with human faecal slurries from 5 healthy donors.					
35537603	2	103	theme	intestinal	374:383	arg1	metabolism					385:394	intestinal metabolism	374:394	intestinal metabolism	374:394	Traditionally, this herb is administered orally, and thus it undergoes intestinal metabolism.					
35537603	1	104	theme	tract	283:287	arg1	disturbances					289:300	the common cold, throat irritation, and upper respiratory tract disturbances	225:300	the common cold, throat irritation, and upper respiratory tract disturbances	225:300	ETHNOPHARMACOLOGICAL RELEVANCE The linden flower (Tiliae flos) has been used for centuries to treat and relieve symptoms of the common cold, throat irritation, and upper respiratory tract disturbances.					
35537603	11	105	theme	LPS-stimulated	1604:1617	arg1	neutrophils					1619:1629	LPS-stimulated neutrophils	1604:1629	LPS-stimulated neutrophils	1604:1629	Raw extract and their metabolites exhibit different levels of inhibition of cytokines production by LPS-stimulated neutrophils, but the reduction of TNF-α production was observed.					
35537603	14	106	theme	Increased	1997:2005	arg1	abundance					2007:2015	Increased abundance	1997:2015	Increased abundance of SCFAs producers	1997:2034	Increased abundance of SCFAs producers may inhibit the production of pro-inflammatory cytokines.					
35537603	14	107	theme	SCFAs	2020:2024	arg1	producers					2026:2034	SCFAs producers	2020:2034	SCFAs producers	2020:2034	Increased abundance of SCFAs producers may inhibit the production of pro-inflammatory cytokines.					
35537603	10	108	theme	human	1394:1398	arg1	composition					1415:1425	human gut microbiota composition	1394:1425	human gut microbiota composition	1394:1425	The extract changed human gut microbiota composition but did not cause dysbiosis (change in the abundance of forty-three genera).					
35537603	10	109	dep	dysbiosis	1445:1453	arg1	change					1456:1461	change	1456:1461	change in the abundance of forty-three genera	1456:1500	The extract changed human gut microbiota composition but did not cause dysbiosis (change in the abundance of forty-three genera).					
35537603	13	110	theme	microbiota	1917:1926	arg1	changes					1940:1946	microbiota composition changes	1917:1946	microbiota composition changes	1917:1946	The anti-inflammatory effect might be linked to both microbiota composition changes and direct activity of bioavailable metabolites.					
35537603	12	111	theme	bacteria	1821:1828	arg1	abundance					1808:1816	the abundance	1804:1816	the abundance of bacteria responsible for SCFAs production	1804:1861	CONCLUSIONS The linden flower extract has a beneficial influence on human gut microbiota because it promotes increasing the abundance of bacteria responsible for SCFAs production.					
35537603	4	112	theme	gut	588:590	arg1	microbiota					592:601	human gut microbiota	582:601	human gut microbiota	582:601	AIM OF THE STUDY The study aimed to determine the interaction between human gut microbiota and the linden flower extracts, resulting in the biotransformation of the extract's constituents and changes in the microbiota composition.					
35537603	3	113	theme	gut	496:498	arg1	microbiota					500:509	human gut microbiota	490:509	human gut microbiota	490:509	Although it is pharmacopeial plant material, there are no reports about its interaction with human gut microbiota.					
36689102	2	0	theme	key	470:472	arg1	factors					474:480	key factors	470:480	key factors that caused the differences	470:508	We aim to demonstrate that agar and gellan gum differently affect the change in root elongation in Arabidopsis thaliana by polyploidization and examined the physico-chemical parameters in each gelling agent to elucidate key factors that caused the differences.					
36689102	10	1	theme	root	1281:1284	arg1	elongation					1286:1295	the root elongation	1277:1295	the root elongation in tetraploids and octoploids	1277:1325	Subsequently, the polyploids were grown on agar media with additional aluminum, on which the root elongation in tetraploids and octoploids was significantly suppressed.					
36689102	1	2	theme	polyploidy-dependent	211:230	arg1	growth					232:237	polyploidy-dependent growth	211:237	polyploidy-dependent growth in plants	211:247	Agar and gellan gum have been considered to have different effects on polyploidy-dependent growth in plants.					
36689102	0	3	theme	root	54:57	arg1	elongation					59:68	root elongation	54:68	root elongation in Arabidopsis thaliana by polyploidization	54:112	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.					
36689102	0	4	from	effects	10:16	arg1	change					44:49	change	44:49	change in root elongation in Arabidopsis thaliana by polyploidization	44:112	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.					
36689102	7	5	theme	medium	913:918	arg1	potential					878:886	Water potential	872:886	Water potential	872:886	Water potential and gel hardness of agar medium were significantly higher than those of gellan gum medium.					
36689102	7	5	theme	medium	913:918	arg1	hardness					896:903	gel hardness	892:903	gel hardness	892:903	Water potential and gel hardness of agar medium were significantly higher than those of gellan gum medium.					
36689102	6	6	theme	trace	810:814	arg1	elements					816:823	trace elements	810:823	trace elements	810:823	Then water potential, gel hardness, and trace elements of each medium were quantified in each medium.					
36689102	11	7	theme	root	1436:1439	arg1	elongation					1441:1450	root elongation	1436:1450	root elongation	1436:1450	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	8	8	from	change	1072:1077	arg1	growth					1103:1108	polyploidy-dependent growth	1082:1108	polyploidy-dependent growth	1082:1108	The decrease in water potential and gel hardness in agar medium, however, did not affect the change in polyploidy-dependent growth.					
36689102	0	9	theme	Arabidopsis	73:83	arg1	thaliana					85:92	Arabidopsis thaliana	73:92	Arabidopsis thaliana	73:92	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.					
36689102	2	10	from	parameters	424:433	arg1	agent					451:455	each gelling agent	438:455	each gelling agent	438:455	We aim to demonstrate that agar and gellan gum differently affect the change in root elongation in Arabidopsis thaliana by polyploidization and examined the physico-chemical parameters in each gelling agent to elucidate key factors that caused the differences.					
36689102	11	11	theme	elongation	1441:1450	arg1	growth					1426:1431	growth	1426:1431	growth of root elongation	1426:1450	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	11	12	theme	gellan	1611:1616	arg1	due					1636:1638	due	1636:1638	due	1636:1638	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	11	12	theme	gellan	1611:1616	arg1	gum					1618:1620	the gellan gum	1607:1620	the gellan gum	1607:1620	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	6	13	theme	water	775:779	arg1	potential					781:789	water potential	775:789	water potential	775:789	Then water potential, gel hardness, and trace elements of each medium were quantified in each medium.					
36689102	11	14	theme	polyploidy-dependent	1544:1563	arg1	growth					1565:1570	polyploidy-dependent growth	1544:1570	polyploidy-dependent growth	1544:1570	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	10	15	theme	agar	1231:1234	arg1	media					1236:1240	agar media	1231:1240	agar media	1231:1240	Subsequently, the polyploids were grown on agar media with additional aluminum, on which the root elongation in tetraploids and octoploids was significantly suppressed.					
36689102	5	16	theme	root	714:717	arg1	elongation					719:728	root elongation	714:728	root elongation of polyploids	714:742	As a result, agar promoted root elongation of polyploids more than the gellan gum.					
36689102	5	16	theme	root	714:717	arg1	result					692:697	a result	690:697	a result	690:697	As a result, agar promoted root elongation of polyploids more than the gellan gum.					
36689102	7	17	theme	agar	908:911	arg1	medium					913:918	agar medium	908:918	agar medium	908:918	Water potential and gel hardness of agar medium were significantly higher than those of gellan gum medium.					
36689102	7	18	theme	gellan	960:965	arg1	medium					971:976	gellan gum medium	960:976	gellan gum medium	960:976	Water potential and gel hardness of agar medium were significantly higher than those of gellan gum medium.					
36689102	10	19	theme	additional	1247:1256	arg1	aluminum					1258:1265	additional aluminum	1247:1265	additional aluminum	1247:1265	Subsequently, the polyploids were grown on agar media with additional aluminum, on which the root elongation in tetraploids and octoploids was significantly suppressed.					
36689102	11	20	from	aluminum	1595:1602	arg1	due					1636:1638	due	1636:1638	due	1636:1638	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	11	20	from	aluminum	1595:1602	arg1	gum					1618:1620	the gellan gum	1607:1620	the gellan gum	1607:1620	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	8	21	theme	water	995:999	arg1	potential					1001:1009	water potential	995:1009	water potential	995:1009	The decrease in water potential and gel hardness in agar medium, however, did not affect the change in polyploidy-dependent growth.					
36689102	2	22	from	change	320:325	arg1	elongation					335:344	root elongation	330:344	root elongation	330:344	We aim to demonstrate that agar and gellan gum differently affect the change in root elongation in Arabidopsis thaliana by polyploidization and examined the physico-chemical parameters in each gelling agent to elucidate key factors that caused the differences.					
36689102	2	22	from	change	320:325	arg1	thaliana					361:368	Arabidopsis thaliana	349:368	Arabidopsis thaliana	349:368	We aim to demonstrate that agar and gellan gum differently affect the change in root elongation in Arabidopsis thaliana by polyploidization and examined the physico-chemical parameters in each gelling agent to elucidate key factors that caused the differences.					
36689102	3	23	theme	polyploid	516:524	arg1	strain					526:531	Each polyploid strain	511:531	Each polyploid strain	511:531	Each polyploid strain was cultured vertically on agar and gellan gum solidified medium under fixed conditions.					
36689102	2	24	theme	Arabidopsis	349:359	arg1	thaliana					361:368	Arabidopsis thaliana	349:368	Arabidopsis thaliana	349:368	We aim to demonstrate that agar and gellan gum differently affect the change in root elongation in Arabidopsis thaliana by polyploidization and examined the physico-chemical parameters in each gelling agent to elucidate key factors that caused the differences.					
36689102	6	25	theme	medium	833:838	arg1	elements					816:823	trace elements	810:823	trace elements	810:823	Then water potential, gel hardness, and trace elements of each medium were quantified in each medium.					
36689102	6	25	theme	medium	833:838	arg1	potential					781:789	water potential	775:789	water potential	775:789	Then water potential, gel hardness, and trace elements of each medium were quantified in each medium.					
36689102	6	25	theme	medium	833:838	arg1	hardness					796:803	gel hardness	792:803	gel hardness	792:803	Then water potential, gel hardness, and trace elements of each medium were quantified in each medium.					
36689102	8	26	theme	gel	1015:1017	arg1	hardness					1019:1026	gel hardness	1015:1026	gel hardness	1015:1026	The decrease in water potential and gel hardness in agar medium, however, did not affect the change in polyploidy-dependent growth.					
36689102	11	27	from	change	1416:1421	arg1	growth					1426:1431	growth	1426:1431	growth of root elongation	1426:1450	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	11	27	from	change	1416:1421	arg1	thaliana					1458:1465	A. thaliana	1455:1465	A. thaliana	1455:1465	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	7	28	theme	gel	892:894	arg1	hardness					896:903	gel hardness	892:903	gel hardness	892:903	Water potential and gel hardness of agar medium were significantly higher than those of gellan gum medium.					
36689102	0	29	theme	Different	0:8	arg1	effects					10:16	Different effects	0:16	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.	0:139	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.					
36689102	11	30	from	change	1534:1539	arg1	growth					1565:1570	polyploidy-dependent growth	1544:1570	polyploidy-dependent growth	1544:1570	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	11	31	theme	wall	1648:1651	arg1	composition					1653:1663	cell wall composition	1643:1663	cell wall composition of polyploids	1643:1677	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	11	32	theme	different	1490:1498	arg1	ways					1500:1503	different ways	1490:1503	different ways	1490:1503	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	0	33	theme	gellan	21:26	arg1	effects					10:16	Different effects	0:16	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.	0:139	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.					
36689102	8	34	from	decrease	983:990	arg1	potential					1001:1009	water potential	995:1009	water potential	995:1009	The decrease in water potential and gel hardness in agar medium, however, did not affect the change in polyploidy-dependent growth.					
36689102	8	34	from	decrease	983:990	arg1	hardness					1019:1026	gel hardness	1015:1026	gel hardness	1015:1026	The decrease in water potential and gel hardness in agar medium, however, did not affect the change in polyploidy-dependent growth.					
36689102	8	34	from	decrease	983:990	arg1	medium					1036:1041	agar medium	1031:1041	agar medium	1031:1041	The decrease in water potential and gel hardness in agar medium, however, did not affect the change in polyploidy-dependent growth.					
36689102	3	35	theme	gellan	569:574	arg1	medium					591:596	gellan gum solidified medium	569:596	gellan gum solidified medium	569:596	Each polyploid strain was cultured vertically on agar and gellan gum solidified medium under fixed conditions.					
36689102	9	36	theme	Elemental	1111:1119	arg1	analysis					1121:1128	Elemental analysis	1111:1128	Elemental analysis	1111:1128	Elemental analysis showed that gellan gum contained more aluminum than agar.					
36689102	4	37	theme	Root	622:625	arg1	rate					638:641	Root elongation rate	622:641	Root elongation rate	622:641	Root elongation rate was measured during 4-10 days after sowing.					
36689102	0	38	from	elongation	59:68	arg1	thaliana					85:92	Arabidopsis thaliana	73:92	Arabidopsis thaliana	73:92	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.					
36689102	11	39	theme	gellan	1394:1399	arg1	gum					1401:1403	gellan gum	1394:1403	gellan gum	1394:1403	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	11	40	from	effects	1523:1529	arg1	change					1534:1539	change	1534:1539	change in polyploidy-dependent growth	1534:1570	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	0	41	theme	key	119:121	arg1	role					123:126	the key role	115:126	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.	0:139	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.					
36689102	3	42	theme	gum	576:578	arg1	medium					591:596	gellan gum solidified medium	569:596	gellan gum solidified medium	569:596	Each polyploid strain was cultured vertically on agar and gellan gum solidified medium under fixed conditions.					
36689102	5	43	theme	gellan	758:763	arg1	gum					765:767	the gellan gum	754:767	the gellan gum	754:767	As a result, agar promoted root elongation of polyploids more than the gellan gum.					
36689102	1	44	from	growth	232:237	arg1	plants					242:247	plants	242:247	plants	242:247	Agar and gellan gum have been considered to have different effects on polyploidy-dependent growth in plants.					
36689102	2	45	theme	physico-chemical	407:422	arg1	parameters					424:433	the physico-chemical parameters	403:433	the physico-chemical parameters in each gelling agent	403:455	We aim to demonstrate that agar and gellan gum differently affect the change in root elongation in Arabidopsis thaliana by polyploidization and examined the physico-chemical parameters in each gelling agent to elucidate key factors that caused the differences.					
36689102	7	46	theme	gum	967:969	arg1	medium					971:976	gellan gum medium	960:976	gellan gum medium	960:976	Water potential and gel hardness of agar medium were significantly higher than those of gellan gum medium.					
36689102	4	47	theme	elongation	627:636	arg1	rate					638:641	Root elongation rate	622:641	Root elongation rate	622:641	Root elongation rate was measured during 4-10 days after sowing.					
36689102	2	48	theme	gelling	443:449	arg1	agent					451:455	each gelling agent	438:455	each gelling agent	438:455	We aim to demonstrate that agar and gellan gum differently affect the change in root elongation in Arabidopsis thaliana by polyploidization and examined the physico-chemical parameters in each gelling agent to elucidate key factors that caused the differences.					
36689102	8	49	theme	polyploidy-dependent	1082:1101	arg1	growth					1103:1108	polyploidy-dependent growth	1082:1108	polyploidy-dependent growth	1082:1108	The decrease in water potential and gel hardness in agar medium, however, did not affect the change in polyploidy-dependent growth.					
36689102	0	50	from	change	44:49	arg1	elongation					59:68	root elongation	54:68	root elongation in Arabidopsis thaliana by polyploidization	54:112	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.					
36689102	1	51	dep	Agar	141:144	arg1	gum					157:159	gum	157:159	gum	157:159	Agar and gellan gum have been considered to have different effects on polyploidy-dependent growth in plants.					
36689102	0	52	theme	agar	36:39	arg1	effects					10:16	Different effects	0:16	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.	0:139	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.					
36689102	9	53	contain	contained	1153:1161	arg1	gum					1149:1151	gellan gum	1142:1151	gellan gum	1142:1151	Elemental analysis showed that gellan gum contained more aluminum than agar.					
36689102	9	53	contain	contained	1153:1161	arg2	aluminum					1168:1175	more aluminum	1163:1175	more aluminum	1163:1175	Elemental analysis showed that gellan gum contained more aluminum than agar.					
36689102	11	54	theme	cell	1643:1646	arg1	composition					1653:1663	cell wall composition	1643:1663	cell wall composition of polyploids	1643:1677	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	1	55	contain	have	185:188	arg1	gellan					150:155	gellan	150:155	gellan	150:155	Agar and gellan gum have been considered to have different effects on polyploidy-dependent growth in plants.					
36689102	1	55	contain	have	185:188	arg1	Agar					141:144	Agar	141:144	Agar	141:144	Agar and gellan gum have been considered to have different effects on polyploidy-dependent growth in plants.					
36689102	1	55	contain	have	185:188	arg2	effects					200:206	different effects	190:206	different effects	190:206	Agar and gellan gum have been considered to have different effects on polyploidy-dependent growth in plants.					
36689102	11	56	theme	different	1513:1521	arg1	effects					1523:1529	the different effects	1509:1529	the different effects on change in polyploidy-dependent growth	1509:1570	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	10	57	with	media	1236:1240	arg1	aluminum					1258:1265	additional aluminum	1247:1265	additional aluminum	1247:1265	Subsequently, the polyploids were grown on agar media with additional aluminum, on which the root elongation in tetraploids and octoploids was significantly suppressed.					
36689102	9	58	theme	more	1163:1166	arg1	aluminum					1168:1175	more aluminum	1163:1175	more aluminum	1163:1175	Elemental analysis showed that gellan gum contained more aluminum than agar.					
36689102	3	59	theme	fixed	604:608	arg1	conditions					610:619	fixed conditions	604:619	fixed conditions	604:619	Each polyploid strain was cultured vertically on agar and gellan gum solidified medium under fixed conditions.					
36689102	0	60	dep	gellan	21:26	arg1	gum					28:30	gum	28:30	gum	28:30	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.					
36689102	3	61	theme	solidified	580:589	arg1	medium					591:596	gellan gum solidified medium	569:596	gellan gum solidified medium	569:596	Each polyploid strain was cultured vertically on agar and gellan gum solidified medium under fixed conditions.					
36689102	9	62	theme	gellan	1142:1147	arg1	gum					1149:1151	gellan gum	1142:1151	gellan gum	1142:1151	Elemental analysis showed that gellan gum contained more aluminum than agar.					
36689102	7	63	theme	Water	872:876	arg1	potential					878:886	Water potential	872:886	Water potential	872:886	Water potential and gel hardness of agar medium were significantly higher than those of gellan gum medium.					
36689102	11	64	theme	polyploids	1668:1677	arg1	composition					1653:1663	cell wall composition	1643:1663	cell wall composition of polyploids	1643:1677	These results revealed that agar and gellan gum affect the change in growth of root elongation in A. thaliana by polyploidization in different ways and the different effects on change in polyploidy-dependent growth is partially caused by aluminum in the gellan gum, which may be due to cell wall composition of polyploids.					
36689102	0	65	theme	aluminum	131:138	arg1	role					123:126	the key role	115:126	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.	0:139	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.					
36689102	2	66	theme	gellan	286:291	arg1	gum					293:295	gellan gum	286:295	gellan gum	286:295	We aim to demonstrate that agar and gellan gum differently affect the change in root elongation in Arabidopsis thaliana by polyploidization and examined the physico-chemical parameters in each gelling agent to elucidate key factors that caused the differences.					
36689102	1	67	theme	different	190:198	arg1	effects					200:206	different effects	190:206	different effects	190:206	Agar and gellan gum have been considered to have different effects on polyploidy-dependent growth in plants.					
36689102	6	68	theme	gel	792:794	arg1	hardness					796:803	gel hardness	792:803	gel hardness	792:803	Then water potential, gel hardness, and trace elements of each medium were quantified in each medium.					
36689102	0	69	dep	effects	10:16	arg1	role					123:126	the key role	115:126	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.	0:139	Different effects of gellan gum and agar on change in root elongation in Arabidopsis thaliana by polyploidization: the key role of aluminum.					
36689102	10	70	from	elongation	1286:1295	arg1	octoploids					1316:1325	octoploids	1316:1325	octoploids	1316:1325	Subsequently, the polyploids were grown on agar media with additional aluminum, on which the root elongation in tetraploids and octoploids was significantly suppressed.					
36689102	10	70	from	elongation	1286:1295	arg1	tetraploids					1300:1310	tetraploids	1300:1310	tetraploids	1300:1310	Subsequently, the polyploids were grown on agar media with additional aluminum, on which the root elongation in tetraploids and octoploids was significantly suppressed.					
36689102	2	71	theme	root	330:333	arg1	elongation					335:344	root elongation	330:344	root elongation	330:344	We aim to demonstrate that agar and gellan gum differently affect the change in root elongation in Arabidopsis thaliana by polyploidization and examined the physico-chemical parameters in each gelling agent to elucidate key factors that caused the differences.					
36689102	5	72	theme	polyploids	733:742	arg1	elongation					719:728	root elongation	714:728	root elongation of polyploids	714:742	As a result, agar promoted root elongation of polyploids more than the gellan gum.					
36689102	5	72	theme	polyploids	733:742	arg1	result					692:697	a result	690:697	a result	690:697	As a result, agar promoted root elongation of polyploids more than the gellan gum.					
36689102	8	73	theme	agar	1031:1034	arg1	medium					1036:1041	agar medium	1031:1041	agar medium	1031:1041	The decrease in water potential and gel hardness in agar medium, however, did not affect the change in polyploidy-dependent growth.					
35868757	0	0	theme	temperature-sensing	88:106	arg1	properties					108:117	temperature-sensing properties	88:117	temperature-sensing properties	88:117	Multifunctional cellulose fibers: Intense red upconversion under 1532 nm excitation and temperature-sensing properties.					
35868757	1	1	from	applications	206:217	arg1	materials					231:239	photonic materials	222:239	photonic materials	222:239	The unique properties of upconverting nanoparticles are responsible for their various applications in photonic materials, medicine, analytics, or energy conversion.					
35868757	3	2	from	Er3+	531:534	arg1	structure					545:553	their structure	539:553	their structure	539:553	Nanoparticles showed intense upconversion under 1532 nm excitation wavelength due to Er3+ in their structure.					
35868757	6	3	theme	Tm3+	901:904	arg1	NPs					912:914	NaErF4:Tm3+@NaYF4 NPs	894:914	NaErF4:Tm3+@NaYF4 NPs	894:914	The article demonstrates that cellulose fibers are suitable carriers of NaErF4:Tm3+@NaYF4 NPs.					
35868757	5	4	dep	products	724:731	arg1	products					724:731	The products' composition, morphology, and structure	720:771	The products' composition, morphology, and structure parameters	720:782	The products' composition, morphology, and structure parameters confirmed their requested properties.					
35868757	5	4	dep	products	724:731	arg1	morphology					747:756	morphology	747:756	morphology	747:756	The products' composition, morphology, and structure parameters confirmed their requested properties.					
35868757	5	4	dep	products	724:731	arg1	structure					763:771	structure	763:771	structure	763:771	The products' composition, morphology, and structure parameters confirmed their requested properties.					
35868757	5	4	dep	products	724:731	arg1	composition					734:744	composition	734:744	composition	734:744	The products' composition, morphology, and structure parameters confirmed their requested properties.					
35868757	2	5	theme	NaErF4	303:308	arg1	nanoparticles					332:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	In this work, the NaErF4:Tm3+@NaYF4 core@shell nanoparticles were synthesized by reaction in high-boiling point solvents and incorporated into cellulose fibers.					
35868757	7	6	theme	cellulose	1033:1041	arg1	fibers					1043:1048	temperature-sensing cellulose fibers	1013:1048	temperature-sensing cellulose fibers	1013:1048	We also show that the temperature-dependent emission of Er3+ ions allows for the preparation of temperature-sensing cellulose fibers.					
35868757	4	7	theme	typical	616:622	arg1	luminescence					630:641	the typical green luminescence	612:641	the typical green luminescence of Er3+ ions	612:654	Additional co-doping with Tm3+ ions allowed to shift of the typical green luminescence of Er3+ ions to red especially demanded in anti-counterfeiting applications.					
35868757	2	8	theme	core	321:324	arg1	nanoparticles					332:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	In this work, the NaErF4:Tm3+@NaYF4 core@shell nanoparticles were synthesized by reaction in high-boiling point solvents and incorporated into cellulose fibers.					
35868757	4	9	theme	ions	651:654	arg1	luminescence					630:641	the typical green luminescence	612:641	the typical green luminescence of Er3+ ions	612:654	Additional co-doping with Tm3+ ions allowed to shift of the typical green luminescence of Er3+ ions to red especially demanded in anti-counterfeiting applications.					
35868757	1	10	theme	various	198:204	arg1	applications					206:217	their various applications	192:217	their various applications in photonic materials	192:239	The unique properties of upconverting nanoparticles are responsible for their various applications in photonic materials, medicine, analytics, or energy conversion.					
35868757	7	11	theme	ions	978:981	arg1	emission					961:968	the temperature-dependent emission	935:968	the temperature-dependent emission of Er3+ ions	935:981	We also show that the temperature-dependent emission of Er3+ ions allows for the preparation of temperature-sensing cellulose fibers.					
35868757	4	12	theme	green	624:628	arg1	luminescence					630:641	the typical green luminescence	612:641	the typical green luminescence of Er3+ ions	612:654	Additional co-doping with Tm3+ ions allowed to shift of the typical green luminescence of Er3+ ions to red especially demanded in anti-counterfeiting applications.					
35868757	2	13	theme	point	391:395	arg1	solvents					397:404	high-boiling point solvents	378:404	high-boiling point solvents	378:404	In this work, the NaErF4:Tm3+@NaYF4 core@shell nanoparticles were synthesized by reaction in high-boiling point solvents and incorporated into cellulose fibers.					
35868757	0	14	theme	cellulose	16:24	arg1	fibers					26:31	Multifunctional cellulose fibers	0:31	Multifunctional cellulose fibers: Intense red upconversion under 1532 nm excitation and temperature-sensing properties.	0:118	Multifunctional cellulose fibers: Intense red upconversion under 1532 nm excitation and temperature-sensing properties.					
35868757	2	15	theme	NaYF4	315:319	arg1	nanoparticles					332:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	In this work, the NaErF4:Tm3+@NaYF4 core@shell nanoparticles were synthesized by reaction in high-boiling point solvents and incorporated into cellulose fibers.					
35868757	2	16	theme	high-boiling	378:389	arg1	solvents					397:404	high-boiling point solvents	378:404	high-boiling point solvents	378:404	In this work, the NaErF4:Tm3+@NaYF4 core@shell nanoparticles were synthesized by reaction in high-boiling point solvents and incorporated into cellulose fibers.					
35868757	0	17	theme	Multifunctional	0:14	arg1	fibers					26:31	Multifunctional cellulose fibers	0:31	Multifunctional cellulose fibers: Intense red upconversion under 1532 nm excitation and temperature-sensing properties.	0:118	Multifunctional cellulose fibers: Intense red upconversion under 1532 nm excitation and temperature-sensing properties.					
35868757	2	18	from	reaction	366:373	arg1	solvents					397:404	high-boiling point solvents	378:404	high-boiling point solvents	378:404	In this work, the NaErF4:Tm3+@NaYF4 core@shell nanoparticles were synthesized by reaction in high-boiling point solvents and incorporated into cellulose fibers.					
35868757	2	19	theme	@	314:314	arg1	nanoparticles					332:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	In this work, the NaErF4:Tm3+@NaYF4 core@shell nanoparticles were synthesized by reaction in high-boiling point solvents and incorporated into cellulose fibers.					
35868757	1	20	theme	photonic	222:229	arg1	materials					231:239	photonic materials	222:239	photonic materials	222:239	The unique properties of upconverting nanoparticles are responsible for their various applications in photonic materials, medicine, analytics, or energy conversion.					
35868757	4	21	theme	Tm3+	582:585	arg1	ions					587:590	Tm3+ ions	582:590	Tm3+ ions allowed to shift of the typical green luminescence of Er3+ ions to red	582:661	Additional co-doping with Tm3+ ions allowed to shift of the typical green luminescence of Er3+ ions to red especially demanded in anti-counterfeiting applications.					
35868757	6	22	theme	cellulose	852:860	arg1	fibers					862:867	cellulose fibers	852:867	cellulose fibers	852:867	The article demonstrates that cellulose fibers are suitable carriers of NaErF4:Tm3+@NaYF4 NPs.					
35868757	6	22	theme	cellulose	852:860	arg1	carriers					882:889	suitable carriers	873:889	suitable carriers of NaErF4:Tm3+@NaYF4 NPs	873:914	The article demonstrates that cellulose fibers are suitable carriers of NaErF4:Tm3+@NaYF4 NPs.					
35868757	2	23	theme	Tm3+	310:313	arg1	nanoparticles					332:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	In this work, the NaErF4:Tm3+@NaYF4 core@shell nanoparticles were synthesized by reaction in high-boiling point solvents and incorporated into cellulose fibers.					
35868757	6	24	theme	suitable	873:880	arg1	fibers					862:867	cellulose fibers	852:867	cellulose fibers	852:867	The article demonstrates that cellulose fibers are suitable carriers of NaErF4:Tm3+@NaYF4 NPs.					
35868757	6	24	theme	suitable	873:880	arg1	carriers					882:889	suitable carriers	873:889	suitable carriers of NaErF4:Tm3+@NaYF4 NPs	873:914	The article demonstrates that cellulose fibers are suitable carriers of NaErF4:Tm3+@NaYF4 NPs.					
35868757	2	25	theme	cellulose	428:436	arg1	fibers					438:443	cellulose fibers	428:443	cellulose fibers	428:443	In this work, the NaErF4:Tm3+@NaYF4 core@shell nanoparticles were synthesized by reaction in high-boiling point solvents and incorporated into cellulose fibers.					
35868757	4	26	with	co-doping	567:575	arg1	ions					587:590	Tm3+ ions	582:590	Tm3+ ions allowed to shift of the typical green luminescence of Er3+ ions to red	582:661	Additional co-doping with Tm3+ ions allowed to shift of the typical green luminescence of Er3+ ions to red especially demanded in anti-counterfeiting applications.					
35868757	6	27	theme	NPs	912:914	arg1	fibers					862:867	cellulose fibers	852:867	cellulose fibers	852:867	The article demonstrates that cellulose fibers are suitable carriers of NaErF4:Tm3+@NaYF4 NPs.					
35868757	6	27	theme	NPs	912:914	arg1	carriers					882:889	suitable carriers	873:889	suitable carriers of NaErF4:Tm3+@NaYF4 NPs	873:914	The article demonstrates that cellulose fibers are suitable carriers of NaErF4:Tm3+@NaYF4 NPs.					
35868757	1	28	theme	unique	124:129	arg1	responsible					176:186	responsible	176:186	responsible	176:186	The unique properties of upconverting nanoparticles are responsible for their various applications in photonic materials, medicine, analytics, or energy conversion.					
35868757	1	28	theme	unique	124:129	arg1	properties					131:140	The unique properties	120:140	The unique properties of upconverting nanoparticles	120:170	The unique properties of upconverting nanoparticles are responsible for their various applications in photonic materials, medicine, analytics, or energy conversion.					
35868757	3	29	theme	1532 nm	494:500	arg1	wavelength					513:522	1532 nm excitation wavelength	494:522	1532 nm excitation wavelength due to Er3+ in their structure	494:553	Nanoparticles showed intense upconversion under 1532 nm excitation wavelength due to Er3+ in their structure.					
35868757	0	30	theme	red	42:44	arg1	upconversion					46:57	Intense red upconversion	34:57	Multifunctional cellulose fibers: Intense red upconversion under 1532 nm excitation and temperature-sensing properties.	0:118	Multifunctional cellulose fibers: Intense red upconversion under 1532 nm excitation and temperature-sensing properties.					
35868757	1	31	from	conversion	273:282	arg1	materials					231:239	photonic materials	222:239	photonic materials	222:239	The unique properties of upconverting nanoparticles are responsible for their various applications in photonic materials, medicine, analytics, or energy conversion.					
35868757	6	32	theme	NaYF4	906:910	arg1	NPs					912:914	NaErF4:Tm3+@NaYF4 NPs	894:914	NaErF4:Tm3+@NaYF4 NPs	894:914	The article demonstrates that cellulose fibers are suitable carriers of NaErF4:Tm3+@NaYF4 NPs.					
35868757	4	33	theme	anti-counterfeiting	686:704	arg1	applications					706:717	anti-counterfeiting applications	686:717	anti-counterfeiting applications	686:717	Additional co-doping with Tm3+ ions allowed to shift of the typical green luminescence of Er3+ ions to red especially demanded in anti-counterfeiting applications.					
35868757	3	34	theme	intense	467:473	arg1	upconversion					475:486	intense upconversion	467:486	intense upconversion	467:486	Nanoparticles showed intense upconversion under 1532 nm excitation wavelength due to Er3+ in their structure.					
35868757	0	35	theme	Intense	34:40	arg1	upconversion					46:57	Intense red upconversion	34:57	Multifunctional cellulose fibers: Intense red upconversion under 1532 nm excitation and temperature-sensing properties.	0:118	Multifunctional cellulose fibers: Intense red upconversion under 1532 nm excitation and temperature-sensing properties.					
35868757	4	36	theme	Additional	556:565	arg1	co-doping					567:575	Additional co-doping	556:575	Additional co-doping with Tm3+ ions allowed to shift of the typical green luminescence of Er3+ ions to red	556:661	Additional co-doping with Tm3+ ions allowed to shift of the typical green luminescence of Er3+ ions to red especially demanded in anti-counterfeiting applications.					
35868757	6	37	theme	@	905:905	arg1	NPs					912:914	NaErF4:Tm3+@NaYF4 NPs	894:914	NaErF4:Tm3+@NaYF4 NPs	894:914	The article demonstrates that cellulose fibers are suitable carriers of NaErF4:Tm3+@NaYF4 NPs.					
35868757	1	38	from	medicine	242:249	arg1	materials					231:239	photonic materials	222:239	photonic materials	222:239	The unique properties of upconverting nanoparticles are responsible for their various applications in photonic materials, medicine, analytics, or energy conversion.					
35868757	5	39	theme	requested	800:808	arg1	properties					810:819	their requested properties	794:819	their requested properties	794:819	The products' composition, morphology, and structure parameters confirmed their requested properties.					
35868757	5	40	theme	products	724:731	arg1	parameters					773:782	The products' composition, morphology, and structure parameters	720:782	The products' composition, morphology, and structure parameters	720:782	The products' composition, morphology, and structure parameters confirmed their requested properties.					
35868757	3	41	theme	excitation	502:511	arg1	wavelength					513:522	1532 nm excitation wavelength	494:522	1532 nm excitation wavelength due to Er3+ in their structure	494:553	Nanoparticles showed intense upconversion under 1532 nm excitation wavelength due to Er3+ in their structure.					
35868757	2	42	theme	shell	326:330	arg1	nanoparticles					332:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	In this work, the NaErF4:Tm3+@NaYF4 core@shell nanoparticles were synthesized by reaction in high-boiling point solvents and incorporated into cellulose fibers.					
35868757	7	43	theme	temperature-dependent	939:959	arg1	emission					961:968	the temperature-dependent emission	935:968	the temperature-dependent emission of Er3+ ions	935:981	We also show that the temperature-dependent emission of Er3+ ions allows for the preparation of temperature-sensing cellulose fibers.					
35868757	7	44	theme	Er3+	973:976	arg1	ions					978:981	Er3+ ions	973:981	Er3+ ions	973:981	We also show that the temperature-dependent emission of Er3+ ions allows for the preparation of temperature-sensing cellulose fibers.					
35868757	1	45	from	analytics	252:260	arg1	materials					231:239	photonic materials	222:239	photonic materials	222:239	The unique properties of upconverting nanoparticles are responsible for their various applications in photonic materials, medicine, analytics, or energy conversion.					
35868757	6	46	theme	NaErF4	894:899	arg1	NPs					912:914	NaErF4:Tm3+@NaYF4 NPs	894:914	NaErF4:Tm3+@NaYF4 NPs	894:914	The article demonstrates that cellulose fibers are suitable carriers of NaErF4:Tm3+@NaYF4 NPs.					
35868757	1	47	theme	upconverting	145:156	arg1	nanoparticles					158:170	upconverting nanoparticles	145:170	upconverting nanoparticles	145:170	The unique properties of upconverting nanoparticles are responsible for their various applications in photonic materials, medicine, analytics, or energy conversion.					
35868757	4	48	theme	luminescence	630:641	arg1	shift					603:607	shift	603:607	shift of the typical green luminescence of Er3+ ions to red	603:661	Additional co-doping with Tm3+ ions allowed to shift of the typical green luminescence of Er3+ ions to red especially demanded in anti-counterfeiting applications.					
35868757	0	49	dep	fibers	26:31	arg1	upconversion					46:57	Intense red upconversion	34:57	Multifunctional cellulose fibers: Intense red upconversion under 1532 nm excitation and temperature-sensing properties.	0:118	Multifunctional cellulose fibers: Intense red upconversion under 1532 nm excitation and temperature-sensing properties.					
35868757	7	50	theme	fibers	1043:1048	arg1	preparation					998:1008	the preparation	994:1008	the preparation of temperature-sensing cellulose fibers	994:1048	We also show that the temperature-dependent emission of Er3+ ions allows for the preparation of temperature-sensing cellulose fibers.					
35868757	1	51	theme	nanoparticles	158:170	arg1	responsible					176:186	responsible	176:186	responsible	176:186	The unique properties of upconverting nanoparticles are responsible for their various applications in photonic materials, medicine, analytics, or energy conversion.					
35868757	1	51	theme	nanoparticles	158:170	arg1	properties					131:140	The unique properties	120:140	The unique properties of upconverting nanoparticles	120:170	The unique properties of upconverting nanoparticles are responsible for their various applications in photonic materials, medicine, analytics, or energy conversion.					
35868757	2	52	theme	@	325:325	arg1	nanoparticles					332:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	the NaErF4:Tm3+@NaYF4 core@shell nanoparticles	299:344	In this work, the NaErF4:Tm3+@NaYF4 core@shell nanoparticles were synthesized by reaction in high-boiling point solvents and incorporated into cellulose fibers.					
35868757	4	53	theme	Er3+	646:649	arg1	ions					651:654	Er3+ ions	646:654	Er3+ ions	646:654	Additional co-doping with Tm3+ ions allowed to shift of the typical green luminescence of Er3+ ions to red especially demanded in anti-counterfeiting applications.					
35868757	0	54	theme	1532 nm	65:71	arg1	excitation					73:82	1532 nm excitation	65:82	1532 nm excitation	65:82	Multifunctional cellulose fibers: Intense red upconversion under 1532 nm excitation and temperature-sensing properties.					
35868757	7	55	theme	temperature-sensing	1013:1031	arg1	fibers					1043:1048	temperature-sensing cellulose fibers	1013:1048	temperature-sensing cellulose fibers	1013:1048	We also show that the temperature-dependent emission of Er3+ ions allows for the preparation of temperature-sensing cellulose fibers.					
35868757	1	56	theme	energy	266:271	arg1	conversion					273:282	energy conversion	266:282	energy conversion	266:282	The unique properties of upconverting nanoparticles are responsible for their various applications in photonic materials, medicine, analytics, or energy conversion.					
36355295	2	0	theme	cartilage	522:530	arg1	functions					446:454	their functions	440:454	their functions in normal healthy cartilage as well as pathological conditions of cartilage	440:530	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	2	0	theme	cartilage	522:530	arg1	conditions					508:517	pathological conditions	495:517	their functions in normal healthy cartilage as well as pathological conditions of cartilage	440:530	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	2	1	from	functions	446:454	arg1	cartilage					474:482	normal healthy cartilage	459:482	normal healthy cartilage	459:482	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	2	2	theme	multimeric	376:385	arg1	complexes					387:395	multimeric complexes	376:395	multimeric complexes	376:395	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	2	3	from	cartilage	315:323	arg1	purification					285:296	purification	285:296	purification	285:296	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	2	3	from	cartilage	315:323	arg1	isolation					271:279	isolation	271:279	isolation	271:279	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	3	4	theme	engineering	550:560	arg1	efforts					562:568	Cartilage tissue engineering efforts	533:568	Cartilage tissue engineering efforts	533:568	Cartilage tissue engineering efforts rely on the comprehensive understanding of the composition of cartilage and the function of each of the protein constituents.					
36355295	3	5	theme	comprehensive	582:594	arg1	understanding					596:608	the comprehensive understanding	578:608	the comprehensive understanding of the composition of cartilage	578:640	Cartilage tissue engineering efforts rely on the comprehensive understanding of the composition of cartilage and the function of each of the protein constituents.					
36355295	0	6	from	Cartilage	52:60	arg1	Isolation					17:25	Isolation	17:25	Isolation	17:25	Purification and Isolation of Proteins from Hyaline Cartilage.					
36355295	0	6	from	Cartilage	52:60	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and Isolation of Proteins from Hyaline Cartilage.					
36355295	2	7	theme	proteins	301:308	arg1	purification					285:296	purification	285:296	purification	285:296	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	2	7	theme	proteins	301:308	arg1	isolation					271:279	isolation	271:279	isolation	271:279	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	1	8	theme	steps	175:179	arg1	series					145:150	a series	143:150	a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting	143:264	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	1	9	theme	chemical	155:162	arg1	steps					175:179	chemical extraction steps	155:179	chemical extraction steps	155:179	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	1	9	theme	chemical	155:162	arg1	immunoblotting					251:264	immunoblotting	251:264	immunoblotting	251:264	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	1	9	theme	chemical	155:162	arg1	spectrometry					234:245	mass spectrometry	229:245	mass spectrometry	229:245	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	2	10	from	conditions	508:517	arg1	cartilage					474:482	normal healthy cartilage	459:482	normal healthy cartilage	459:482	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	1	11	theme	hyaline	77:83	arg1	cartilage					98:106	hyaline or articular cartilage	77:106	cartilage	98:106	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	1	12	theme	extraction	164:173	arg1	steps					175:179	chemical extraction steps	155:179	chemical extraction steps	155:179	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	1	12	theme	extraction	164:173	arg1	immunoblotting					251:264	immunoblotting	251:264	immunoblotting	251:264	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	1	12	theme	extraction	164:173	arg1	spectrometry					234:245	mass spectrometry	229:245	mass spectrometry	229:245	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	3	13	theme	composition	617:627	arg1	function					650:657	the function	646:657	the function of each of the protein constituents	646:693	Cartilage tissue engineering efforts rely on the comprehensive understanding of the composition of cartilage and the function of each of the protein constituents.					
36355295	3	13	theme	composition	617:627	arg1	understanding					596:608	the comprehensive understanding	578:608	the comprehensive understanding of the composition of cartilage	578:640	Cartilage tissue engineering efforts rely on the comprehensive understanding of the composition of cartilage and the function of each of the protein constituents.					
36355295	2	14	theme	proteins	410:417	arg1	proteins					363:370	specific proteins	354:370	specific proteins	354:370	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	2	14	theme	proteins	410:417	arg1	complexes					387:395	multimeric complexes	376:395	multimeric complexes	376:395	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	1	15	theme	articular	88:96	arg1	cartilage					98:106	hyaline or articular cartilage	77:106	cartilage	98:106	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	2	16	theme	cartilage	400:408	arg1	proteins					410:417	cartilage proteins	400:417	cartilage proteins	400:417	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	2	17	theme	healthy	466:472	arg1	cartilage					474:482	normal healthy cartilage	459:482	normal healthy cartilage	459:482	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	1	18	theme	various	185:191	arg1	immunoblotting					251:264	immunoblotting	251:264	immunoblotting	251:264	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	1	18	theme	various	185:191	arg1	techniques					208:217	various identification techniques	185:217	various identification techniques	185:217	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	1	18	theme	various	185:191	arg1	spectrometry					234:245	mass spectrometry	229:245	mass spectrometry	229:245	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	2	19	theme	normal	459:464	arg1	cartilage					474:482	normal healthy cartilage	459:482	normal healthy cartilage	459:482	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	1	20	theme	identification	193:206	arg1	immunoblotting					251:264	immunoblotting	251:264	immunoblotting	251:264	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	1	20	theme	identification	193:206	arg1	techniques					208:217	various identification techniques	185:217	various identification techniques	185:217	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	1	20	theme	identification	193:206	arg1	spectrometry					234:245	mass spectrometry	229:245	mass spectrometry	229:245	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	3	21	theme	tissue	543:548	arg1	efforts					562:568	Cartilage tissue engineering efforts	533:568	Cartilage tissue engineering efforts	533:568	Cartilage tissue engineering efforts rely on the comprehensive understanding of the composition of cartilage and the function of each of the protein constituents.					
36355295	3	22	theme	Cartilage	533:541	arg1	efforts					562:568	Cartilage tissue engineering efforts	533:568	Cartilage tissue engineering efforts	533:568	Cartilage tissue engineering efforts rely on the comprehensive understanding of the composition of cartilage and the function of each of the protein constituents.					
36355295	2	23	dep	isolation	271:279	arg1	The					267:269	The	267:269	The	267:269	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	1	24	theme	techniques	208:217	arg1	series					145:150	a series	143:150	a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting	143:264	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	0	25	theme	Proteins	30:37	arg1	Isolation					17:25	Isolation	17:25	Isolation	17:25	Purification and Isolation of Proteins from Hyaline Cartilage.					
36355295	0	25	theme	Proteins	30:37	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and Isolation of Proteins from Hyaline Cartilage.					
36355295	2	26	theme	pathological	495:506	arg1	conditions					508:517	pathological conditions	495:517	their functions in normal healthy cartilage as well as pathological conditions of cartilage	440:530	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	3	27	theme	protein	674:680	arg1	constituents					682:693	the protein constituents	670:693	the protein constituents	670:693	Cartilage tissue engineering efforts rely on the comprehensive understanding of the composition of cartilage and the function of each of the protein constituents.					
36355295	3	28	theme	cartilage	632:640	arg1	composition					617:627	the composition	613:627	the composition of cartilage	613:640	Cartilage tissue engineering efforts rely on the comprehensive understanding of the composition of cartilage and the function of each of the protein constituents.					
36355295	2	29	theme	proteins	363:370	arg1	study					345:349	the study	341:349	the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage	341:530	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	0	30	theme	Hyaline	44:50	arg1	Cartilage					52:60	Hyaline Cartilage	44:60	Hyaline Cartilage	44:60	Purification and Isolation of Proteins from Hyaline Cartilage.					
36355295	2	31	theme	specific	354:361	arg1	proteins					363:370	specific proteins	354:370	specific proteins	354:370	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36355295	1	32	from	cartilage	98:106	arg1	Proteins					63:70	Proteins	63:70	Proteins from hyaline or articular cartilage	63:106	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	1	33	theme	mass	229:232	arg1	spectrometry					234:245	mass spectrometry	229:245	mass spectrometry	229:245	Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting.					
36355295	3	34	theme	each	662:665	arg1	function					650:657	the function	646:657	the function of each of the protein constituents	646:693	Cartilage tissue engineering efforts rely on the comprehensive understanding of the composition of cartilage and the function of each of the protein constituents.					
36355295	3	34	theme	each	662:665	arg1	understanding					596:608	the comprehensive understanding	578:608	the comprehensive understanding of the composition of cartilage	578:640	Cartilage tissue engineering efforts rely on the comprehensive understanding of the composition of cartilage and the function of each of the protein constituents.					
36355295	2	35	theme	complexes	387:395	arg1	study					345:349	the study	341:349	the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage	341:530	The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage.					
36306917	13	0	theme	adsorption	1881:1890	arg1	process					1892:1898	the adsorption process	1877:1898	the adsorption process of the system	1877:1912	In this study, a novel and effective adsorbent for phosphorus removal was produced in contaminated water, and the analysis of the adsorption process of the system provides new insights for related research.					
36306917	1	1	with	composite	188:196	arg1	adsorption					213:222	efficient adsorption	203:222	efficient adsorption of phosphate in water	203:244	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite with efficient adsorption of phosphate in water was prepared and characterized.					
36306917	1	2	from	adsorption	213:222	arg1	water					240:244	water	240:244	water	240:244	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite with efficient adsorption of phosphate in water was prepared and characterized.					
36306917	10	3	theme	dynamic	1322:1328	arg1	experiments					1341:1351	The dynamic adsorption experiments	1318:1351	The dynamic adsorption experiments	1318:1351	The dynamic adsorption experiments showed that the faster the flow rate, the shorter the time for La-CTS-ATP to get saturation, and the maximum adsorption capacity was slightly reduced.					
36306917	13	4	theme	process	1892:1898	arg1	analysis					1865:1872	the analysis	1861:1872	the analysis of the adsorption process of the system	1861:1912	In this study, a novel and effective adsorbent for phosphorus removal was produced in contaminated water, and the analysis of the adsorption process of the system provides new insights for related research.					
36306917	9	5	theme	removal	1244:1250	arg1	rate					1252:1255	the phosphate removal rate	1230:1255	the phosphate removal rate	1230:1255	The excellent reusability of La-CTS-ATP was investigated, after five cycles, the phosphate removal rate and desorption rates were 72.93 % and 77.44 %, respectively.					
36306917	5	6	theme	4-10	697:700	arg1	range					688:692	pH range	685:692	pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-)	685:772	The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-).					
36306917	11	7	theme	ligand	1561:1566	arg1	exchange					1568:1575	ligand exchange	1561:1575	ligand exchange	1561:1575	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	11	7	theme	ligand	1561:1566	arg1	precipitation					1516:1528	The surface precipitation	1504:1528	The surface precipitation	1504:1528	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	11	7	theme	ligand	1561:1566	arg1	mechanisms					1591:1600	the main mechanisms	1582:1600	the main mechanisms affecting its adsorption behavior	1582:1634	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	11	7	theme	ligand	1561:1566	arg1	attraction					1545:1554	electrostatic attraction	1531:1554	electrostatic attraction	1531:1554	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	10	8	theme	adsorption	1462:1471	arg1	capacity					1473:1480	the maximum adsorption capacity	1450:1480	the maximum adsorption capacity	1450:1480	The dynamic adsorption experiments showed that the faster the flow rate, the shorter the time for La-CTS-ATP to get saturation, and the maximum adsorption capacity was slightly reduced.					
36306917	11	9	theme	main	1586:1589	arg1	exchange					1568:1575	ligand exchange	1561:1575	ligand exchange	1561:1575	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	11	9	theme	main	1586:1589	arg1	precipitation					1516:1528	The surface precipitation	1504:1528	The surface precipitation	1504:1528	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	11	9	theme	main	1586:1589	arg1	mechanisms					1591:1600	the main mechanisms	1582:1600	the main mechanisms affecting its adsorption behavior	1582:1634	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	11	9	theme	main	1586:1589	arg1	attraction					1545:1554	electrostatic attraction	1531:1554	electrostatic attraction	1531:1554	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	6	10	theme	main	800:803	arg1	role					805:808	the main role	796:808	the main role	796:808	Chemisorption played the main role in phosphate adsorption from kinetic studies.					
36306917	6	11	theme	phosphate	813:821	arg1	adsorption					823:832	phosphate adsorption	813:832	phosphate adsorption from kinetic studies	813:853	Chemisorption played the main role in phosphate adsorption from kinetic studies.					
36306917	10	12	dep	faster	1369:1374	arg1	rate					1385:1388	the flow rate	1376:1388	the faster the flow rate	1365:1388	The dynamic adsorption experiments showed that the faster the flow rate, the shorter the time for La-CTS-ATP to get saturation, and the maximum adsorption capacity was slightly reduced.					
36306917	9	13	theme	desorption	1261:1270	arg1	rates					1272:1276	desorption rates	1261:1276	desorption rates	1261:1276	The excellent reusability of La-CTS-ATP was investigated, after five cycles, the phosphate removal rate and desorption rates were 72.93 % and 77.44 %, respectively.					
36306917	13	14	theme	system	1907:1912	arg1	process					1892:1898	the adsorption process	1877:1898	the adsorption process of the system	1877:1912	In this study, a novel and effective adsorbent for phosphorus removal was produced in contaminated water, and the analysis of the adsorption process of the system provides new insights for related research.					
36306917	7	15	theme	maximum	944:950	arg1	102.90 mg/g					976:986	102.90 mg/g	976:986	102.90 mg/g	976:986	Isotherm studies illustrated monolayer uptake of adsorbents onto the adsorbent, and the maximum adsorption capacity was 102.90 mg/g, much higher than other La-based adsorbents.					
36306917	7	15	theme	maximum	944:950	arg1	capacity					963:970	the maximum adsorption capacity	940:970	the maximum adsorption capacity	940:970	Isotherm studies illustrated monolayer uptake of adsorbents onto the adsorbent, and the maximum adsorption capacity was 102.90 mg/g, much higher than other La-based adsorbents.					
36306917	13	16	theme	related	1940:1946	arg1	research					1948:1955	related research	1940:1955	related research	1940:1955	In this study, a novel and effective adsorbent for phosphorus removal was produced in contaminated water, and the analysis of the adsorption process of the system provides new insights for related research.					
36306917	8	17	dep	disordered	1104:1113	arg1	ΔS > 0					1116:1121	ΔS > 0	1116:1121	ΔS > 0	1116:1121	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	12	18	dep	wastewater	1693:1702	arg1	wastewater					1714:1723	breeding wastewater	1705:1723	breeding wastewater	1705:1723	The adsorbent exhibited excellent effectiveness in real wastewater (breeding wastewater and domestic wastewater).					
36306917	12	18	dep	wastewater	1693:1702	arg1	wastewater					1738:1747	domestic wastewater	1729:1747	domestic wastewater	1729:1747	The adsorbent exhibited excellent effectiveness in real wastewater (breeding wastewater and domestic wastewater).					
36306917	5	19	theme	disturbance	710:720	arg1	range					688:692	pH range	685:692	pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-)	685:772	The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-).					
36306917	2	20	theme	adsorption	325:334	arg1	kinetics					336:343	adsorption kinetics	325:343	adsorption kinetics	325:343	The adsorption properties were investigated by adsorption kinetics, adsorption isotherms and thermodynamics.					
36306917	7	21	theme	adsorption	952:961	arg1	102.90 mg/g					976:986	102.90 mg/g	976:986	102.90 mg/g	976:986	Isotherm studies illustrated monolayer uptake of adsorbents onto the adsorbent, and the maximum adsorption capacity was 102.90 mg/g, much higher than other La-based adsorbents.					
36306917	7	21	theme	adsorption	952:961	arg1	capacity					963:970	the maximum adsorption capacity	940:970	the maximum adsorption capacity	940:970	Isotherm studies illustrated monolayer uptake of adsorbents onto the adsorbent, and the maximum adsorption capacity was 102.90 mg/g, much higher than other La-based adsorbents.					
36306917	1	22	theme	efficient	203:211	arg1	adsorption					213:222	efficient adsorption	203:222	efficient adsorption of phosphate in water	203:244	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite with efficient adsorption of phosphate in water was prepared and characterized.					
36306917	0	23	theme	phosphate	54:62	arg1	removal					64:70	phosphate removal	54:70	phosphate removal from water	54:81	Lanthanum modified chitosan-attapulgite composite for phosphate removal from water: Performance, mechanisms and applicability.					
36306917	9	24	theme	excellent	1157:1165	arg1	reusability					1167:1177	The excellent reusability	1153:1177	The excellent reusability of La-CTS-ATP	1153:1191	The excellent reusability of La-CTS-ATP was investigated, after five cycles, the phosphate removal rate and desorption rates were 72.93 % and 77.44 %, respectively.					
36306917	3	25	theme	optimal	437:443	arg1	conditions					457:466	the optimal preparation conditions	433:466	the optimal preparation conditions of La-CTS-ATP	433:480	An orthogonal design test was used to explore the optimal preparation conditions of La-CTS-ATP.					
36306917	4	26	theme	effective	568:576	arg1	adsorption					578:587	the effective adsorption	564:587	the effective adsorption of phosphate	564:600	Various characterizations exhibited the successful modification of lanthanum and the effective adsorption of phosphate.					
36306917	5	27	theme	substances	737:746	arg1	4-10					697:700	4-10	697:700	4-10	697:700	The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-).					
36306917	5	27	theme	substances	737:746	arg1	disturbance					710:720	the disturbance	706:720	the disturbance of co-existing substances	706:746	The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-).					
36306917	5	28	theme	pH	685:686	arg1	range					688:692	pH range	685:692	pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-)	685:772	The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-).					
36306917	10	29	theme	maximum	1454:1460	arg1	capacity					1473:1480	the maximum adsorption capacity	1450:1480	the maximum adsorption capacity	1450:1480	The dynamic adsorption experiments showed that the faster the flow rate, the shorter the time for La-CTS-ATP to get saturation, and the maximum adsorption capacity was slightly reduced.					
36306917	8	30	dep	exothermic	1080:1089	arg1	ΔH < 0					1092:1097	ΔH < 0	1092:1097	ΔH < 0	1092:1097	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	3	31	theme	preparation	445:455	arg1	conditions					457:466	the optimal preparation conditions	433:466	the optimal preparation conditions of La-CTS-ATP	433:480	An orthogonal design test was used to explore the optimal preparation conditions of La-CTS-ATP.					
36306917	11	32	theme	surface	1508:1514	arg1	exchange					1568:1575	ligand exchange	1561:1575	ligand exchange	1561:1575	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	11	32	theme	surface	1508:1514	arg1	precipitation					1516:1528	The surface precipitation	1504:1528	The surface precipitation	1504:1528	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	11	32	theme	surface	1508:1514	arg1	mechanisms					1591:1600	the main mechanisms	1582:1600	the main mechanisms affecting its adsorption behavior	1582:1634	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	11	32	theme	surface	1508:1514	arg1	attraction					1545:1554	electrostatic attraction	1531:1554	electrostatic attraction	1531:1554	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	3	33	used	used	417:420	arg2	test					408:411	An orthogonal design test	387:411	An orthogonal design test	387:411	An orthogonal design test was used to explore the optimal preparation conditions of La-CTS-ATP.					
36306917	7	34	theme	La-based	1012:1019	arg1	adsorbents					1021:1030	other La-based adsorbents	1006:1030	other La-based adsorbents	1006:1030	Isotherm studies illustrated monolayer uptake of adsorbents onto the adsorbent, and the maximum adsorption capacity was 102.90 mg/g, much higher than other La-based adsorbents.					
36306917	1	35	theme	phosphate	227:235	arg1	adsorption					213:222	efficient adsorption	203:222	efficient adsorption of phosphate in water	203:244	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite with efficient adsorption of phosphate in water was prepared and characterized.					
36306917	13	36	theme	contaminated	1837:1848	arg1	water					1850:1854	contaminated water	1837:1854	contaminated water	1837:1854	In this study, a novel and effective adsorbent for phosphorus removal was produced in contaminated water, and the analysis of the adsorption process of the system provides new insights for related research.					
36306917	7	37	theme	adsorbents	905:914	arg1	uptake					895:900	monolayer uptake	885:900	monolayer uptake of adsorbents onto the adsorbent	885:933	Isotherm studies illustrated monolayer uptake of adsorbents onto the adsorbent, and the maximum adsorption capacity was 102.90 mg/g, much higher than other La-based adsorbents.					
36306917	9	38	theme	La-CTS-ATP	1182:1191	arg1	reusability					1167:1177	The excellent reusability	1153:1177	The excellent reusability of La-CTS-ATP	1153:1191	The excellent reusability of La-CTS-ATP was investigated, after five cycles, the phosphate removal rate and desorption rates were 72.93 % and 77.44 %, respectively.					
36306917	0	39	theme	chitosan-attapulgite	19:38	arg1	composite					40:48	chitosan-attapulgite composite	19:48	chitosan-attapulgite composite	19:48	Lanthanum modified chitosan-attapulgite composite for phosphate removal from water: Performance, mechanisms and applicability.					
36306917	7	40	theme	monolayer	885:893	arg1	uptake					895:900	monolayer uptake	885:900	monolayer uptake of adsorbents onto the adsorbent	885:933	Isotherm studies illustrated monolayer uptake of adsorbents onto the adsorbent, and the maximum adsorption capacity was 102.90 mg/g, much higher than other La-based adsorbents.					
36306917	2	41	theme	adsorption	346:355	arg1	isotherms					357:365	adsorption isotherms	346:365	adsorption isotherms	346:365	The adsorption properties were investigated by adsorption kinetics, adsorption isotherms and thermodynamics.					
36306917	12	42	theme	domestic	1729:1736	arg1	wastewater					1738:1747	domestic wastewater	1729:1747	domestic wastewater	1729:1747	The adsorbent exhibited excellent effectiveness in real wastewater (breeding wastewater and domestic wastewater).					
36306917	3	43	theme	La-CTS-ATP	471:480	arg1	conditions					457:466	the optimal preparation conditions	433:466	the optimal preparation conditions of La-CTS-ATP	433:480	An orthogonal design test was used to explore the optimal preparation conditions of La-CTS-ATP.					
36306917	4	44	theme	successful	523:532	arg1	modification					534:545	the successful modification	519:545	the successful modification of lanthanum	519:558	Various characterizations exhibited the successful modification of lanthanum and the effective adsorption of phosphate.					
36306917	10	45	dep	shorter	1395:1401	arg1	time					1407:1410	the time	1403:1410	the shorter the time for La-CTS-ATP to get saturation	1391:1443	The dynamic adsorption experiments showed that the faster the flow rate, the shorter the time for La-CTS-ATP to get saturation, and the maximum adsorption capacity was slightly reduced.					
36306917	13	46	theme	phosphorus	1802:1811	arg1	removal					1813:1819	phosphorus removal	1802:1819	phosphorus removal	1802:1819	In this study, a novel and effective adsorbent for phosphorus removal was produced in contaminated water, and the analysis of the adsorption process of the system provides new insights for related research.					
36306917	8	47	from	analysis	1143:1150	arg1	process					1046:1052	The reaction process	1033:1052	The reaction process	1033:1052	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	8	47	from	analysis	1143:1150	arg1	disordered					1104:1113	disordered	1104:1113	disordered	1104:1113	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	8	47	from	analysis	1143:1150	arg1	ΔG < 0					1071:1076	ΔG < 0	1071:1076	ΔG < 0	1071:1076	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	8	47	from	analysis	1143:1150	arg1	exothermic					1080:1089	exothermic	1080:1089	exothermic	1080:1089	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	8	47	from	analysis	1143:1150	arg1	spontaneous					1058:1068	spontaneous	1058:1068	spontaneous	1058:1068	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	11	48	theme	adsorption	1616:1625	arg1	behavior					1627:1634	its adsorption behavior	1612:1634	its adsorption behavior	1612:1634	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	4	49	theme	lanthanum	550:558	arg1	modification					534:545	the successful modification	519:545	the successful modification of lanthanum	519:558	Various characterizations exhibited the successful modification of lanthanum and the effective adsorption of phosphate.					
36306917	4	49	theme	lanthanum	550:558	arg1	adsorption					578:587	the effective adsorption	564:587	the effective adsorption of phosphate	564:600	Various characterizations exhibited the successful modification of lanthanum and the effective adsorption of phosphate.					
36306917	13	50	theme	new	1923:1925	arg1	insights					1927:1934	new insights	1923:1934	new insights for related research	1923:1955	In this study, a novel and effective adsorbent for phosphorus removal was produced in contaminated water, and the analysis of the adsorption process of the system provides new insights for related research.					
36306917	12	51	theme	excellent	1661:1669	arg1	effectiveness					1671:1683	excellent effectiveness	1661:1683	excellent effectiveness	1661:1683	The adsorbent exhibited excellent effectiveness in real wastewater (breeding wastewater and domestic wastewater).					
36306917	0	52	from	water	77:81	arg1	removal					64:70	phosphate removal	54:70	phosphate removal from water	54:81	Lanthanum modified chitosan-attapulgite composite for phosphate removal from water: Performance, mechanisms and applicability.					
36306917	6	53	theme	kinetic	839:845	arg1	studies					847:853	kinetic studies	839:853	kinetic studies	839:853	Chemisorption played the main role in phosphate adsorption from kinetic studies.					
36306917	11	54	theme	electrostatic	1531:1543	arg1	exchange					1568:1575	ligand exchange	1561:1575	ligand exchange	1561:1575	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	11	54	theme	electrostatic	1531:1543	arg1	precipitation					1516:1528	The surface precipitation	1504:1528	The surface precipitation	1504:1528	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	11	54	theme	electrostatic	1531:1543	arg1	attraction					1545:1554	electrostatic attraction	1531:1554	electrostatic attraction	1531:1554	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	11	54	theme	electrostatic	1531:1543	arg1	mechanisms					1591:1600	the main mechanisms	1582:1600	the main mechanisms affecting its adsorption behavior	1582:1634	The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior.					
36306917	5	55	theme	co-existing	725:735	arg1	substances					737:746	co-existing substances	725:746	co-existing substances	725:746	The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-).					
36306917	5	56	theme	excellent	658:666	arg1	stability					668:676	excellent stability	658:676	excellent stability	658:676	The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-).					
36306917	1	57	theme	novel	129:133	arg1	composite					188:196	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite	127:196	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite with efficient adsorption of phosphate in water	127:244	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite with efficient adsorption of phosphate in water was prepared and characterized.					
36306917	8	58	dep	spontaneous	1058:1068	arg1	process					1046:1052	The reaction process	1033:1052	The reaction process	1033:1052	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	8	58	dep	spontaneous	1058:1068	arg1	spontaneous					1058:1068	spontaneous	1058:1068	spontaneous	1058:1068	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	8	58	dep	spontaneous	1058:1068	arg1	ΔG < 0					1071:1076	ΔG < 0	1071:1076	ΔG < 0	1071:1076	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	8	58	dep	spontaneous	1058:1068	arg1	exothermic					1080:1089	exothermic	1080:1089	exothermic	1080:1089	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	8	58	dep	spontaneous	1058:1068	arg1	disordered					1104:1113	disordered	1104:1113	disordered	1104:1113	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	7	59	theme	Isotherm	856:863	arg1	studies					865:871	Isotherm studies	856:871	Isotherm studies	856:871	Isotherm studies illustrated monolayer uptake of adsorbents onto the adsorbent, and the maximum adsorption capacity was 102.90 mg/g, much higher than other La-based adsorbents.					
36306917	4	60	theme	Various	483:489	arg1	characterizations					491:507	Various characterizations	483:507	Various characterizations	483:507	Various characterizations exhibited the successful modification of lanthanum and the effective adsorption of phosphate.					
36306917	12	61	theme	breeding	1705:1712	arg1	wastewater					1714:1723	breeding wastewater	1705:1723	breeding wastewater	1705:1723	The adsorbent exhibited excellent effectiveness in real wastewater (breeding wastewater and domestic wastewater).					
36306917	1	62	theme	lanthanum-modified-chitosan-attapulgite	135:173	arg1	composite					188:196	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite	127:196	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite with efficient adsorption of phosphate in water	127:244	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite with efficient adsorption of phosphate in water was prepared and characterized.					
36306917	7	63	theme	other	1006:1010	arg1	adsorbents					1021:1030	other La-based adsorbents	1006:1030	other La-based adsorbents	1006:1030	Isotherm studies illustrated monolayer uptake of adsorbents onto the adsorbent, and the maximum adsorption capacity was 102.90 mg/g, much higher than other La-based adsorbents.					
36306917	12	64	theme	real	1688:1691	arg1	wastewater					1693:1702	real wastewater	1688:1702	real wastewater (breeding wastewater and domestic wastewater)	1688:1748	The adsorbent exhibited excellent effectiveness in real wastewater (breeding wastewater and domestic wastewater).					
36306917	5	65	theme	batch	607:611	arg1	experiments					613:623	The batch experiments	603:623	The batch experiments	603:623	The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-).					
36306917	10	66	theme	flow	1380:1383	arg1	rate					1385:1388	the flow rate	1376:1388	the faster the flow rate	1365:1388	The dynamic adsorption experiments showed that the faster the flow rate, the shorter the time for La-CTS-ATP to get saturation, and the maximum adsorption capacity was slightly reduced.					
36306917	5	67	dep	4-10	697:700	arg1	SO42-					767:771	SO42-	767:771	SO42-	767:771	The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-).					
36306917	5	67	dep	4-10	697:700	arg1	HCO3-					760:764	HCO3-	760:764	HCO3-	760:764	The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-).					
36306917	5	67	dep	4-10	697:700	arg1	NO3-					754:757	NO3-	754:757	NO3-	754:757	The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-).					
36306917	5	67	dep	4-10	697:700	arg1	Cl-					749:751	Cl-	749:751	Cl-	749:751	The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-).					
36306917	6	68	from	studies	847:853	arg1	adsorption					823:832	phosphate adsorption	813:832	phosphate adsorption from kinetic studies	813:853	Chemisorption played the main role in phosphate adsorption from kinetic studies.					
36306917	3	69	theme	orthogonal	390:399	arg1	test					408:411	An orthogonal design test	387:411	An orthogonal design test	387:411	An orthogonal design test was used to explore the optimal preparation conditions of La-CTS-ATP.					
36306917	8	70	theme	thermodynamic	1129:1141	arg1	analysis					1143:1150	thermodynamic analysis	1129:1150	thermodynamic analysis	1129:1150	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	0	71	dep	modified	10:17	arg1	Performance					84:94	Performance	84:94	Performance	84:94	Lanthanum modified chitosan-attapulgite composite for phosphate removal from water: Performance, mechanisms and applicability.					
36306917	0	71	dep	modified	10:17	arg1	applicability					112:124	applicability	112:124	applicability	112:124	Lanthanum modified chitosan-attapulgite composite for phosphate removal from water: Performance, mechanisms and applicability.					
36306917	0	71	dep	modified	10:17	arg1	mechanisms					97:106	mechanisms	97:106	mechanisms	97:106	Lanthanum modified chitosan-attapulgite composite for phosphate removal from water: Performance, mechanisms and applicability.					
36306917	8	72	theme	reaction	1037:1044	arg1	process					1046:1052	The reaction process	1033:1052	The reaction process	1033:1052	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	8	72	theme	reaction	1037:1044	arg1	disordered					1104:1113	disordered	1104:1113	disordered	1104:1113	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	8	72	theme	reaction	1037:1044	arg1	ΔG < 0					1071:1076	ΔG < 0	1071:1076	ΔG < 0	1071:1076	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	8	72	theme	reaction	1037:1044	arg1	exothermic					1080:1089	exothermic	1080:1089	exothermic	1080:1089	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	8	72	theme	reaction	1037:1044	arg1	spontaneous					1058:1068	spontaneous	1058:1068	spontaneous	1058:1068	The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis.					
36306917	1	73	theme	La-CTS-ATP	176:185	arg1	composite					188:196	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite	127:196	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite with efficient adsorption of phosphate in water	127:244	A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite with efficient adsorption of phosphate in water was prepared and characterized.					
36306917	7	74	theme	higher	994:999	arg1	102.90 mg/g					976:986	102.90 mg/g	976:986	102.90 mg/g	976:986	Isotherm studies illustrated monolayer uptake of adsorbents onto the adsorbent, and the maximum adsorption capacity was 102.90 mg/g, much higher than other La-based adsorbents.					
36306917	7	74	theme	higher	994:999	arg1	capacity					963:970	the maximum adsorption capacity	940:970	the maximum adsorption capacity	940:970	Isotherm studies illustrated monolayer uptake of adsorbents onto the adsorbent, and the maximum adsorption capacity was 102.90 mg/g, much higher than other La-based adsorbents.					
36306917	3	75	theme	design	401:406	arg1	test					408:411	An orthogonal design test	387:411	An orthogonal design test	387:411	An orthogonal design test was used to explore the optimal preparation conditions of La-CTS-ATP.					
36306917	4	76	theme	phosphate	592:600	arg1	modification					534:545	the successful modification	519:545	the successful modification of lanthanum	519:558	Various characterizations exhibited the successful modification of lanthanum and the effective adsorption of phosphate.					
36306917	4	76	theme	phosphate	592:600	arg1	adsorption					578:587	the effective adsorption	564:587	the effective adsorption of phosphate	564:600	Various characterizations exhibited the successful modification of lanthanum and the effective adsorption of phosphate.					
36306917	2	77	theme	adsorption	282:291	arg1	properties					293:302	The adsorption properties	278:302	The adsorption properties	278:302	The adsorption properties were investigated by adsorption kinetics, adsorption isotherms and thermodynamics.					
36306917	9	78	theme	phosphate	1234:1242	arg1	rate					1252:1255	the phosphate removal rate	1230:1255	the phosphate removal rate	1230:1255	The excellent reusability of La-CTS-ATP was investigated, after five cycles, the phosphate removal rate and desorption rates were 72.93 % and 77.44 %, respectively.					
36306917	10	79	theme	adsorption	1330:1339	arg1	experiments					1341:1351	The dynamic adsorption experiments	1318:1351	The dynamic adsorption experiments	1318:1351	The dynamic adsorption experiments showed that the faster the flow rate, the shorter the time for La-CTS-ATP to get saturation, and the maximum adsorption capacity was slightly reduced.					
36529878	10	0	dep	fermented	1654:1662	arg1	must					1649:1652	must	1649:1652	must	1649:1652	PRACTICAL APPLICATION: We found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY) modified the chemical composition of the final young wine.					
36529878	1	1	theme	yeasts	174:179	arg1	use					121:123	use	121:123	use	121:123	Recently, the use and commercial availability of non-Saccharomyces yeasts (NSY) in winemaking to reduce alcohol content have increased.					
36529878	1	1	theme	yeasts	174:179	arg1	availability					140:151	commercial availability	129:151	commercial availability	129:151	Recently, the use and commercial availability of non-Saccharomyces yeasts (NSY) in winemaking to reduce alcohol content have increased.					
36529878	0	2	theme	non-Saccharomyces	81:97	arg1	yeasts					99:104	pectinase-producing non-Saccharomyces yeasts	61:104	pectinase-producing non-Saccharomyces yeasts	61:104	Sensory properties of 6- and 18-month-stored wines made with pectinase-producing non-Saccharomyces yeasts.					
36529878	5	3	theme	higher	799:804	arg1	concentrations					806:819	higher concentrations	799:819	higher concentrations of d-galacturonic acid and glycerol concentration	799:869	Addition of NSY with added pectin resulted in higher concentrations of d-galacturonic acid and glycerol concentration in the wines after 6 months of aging.					
36529878	6	4	theme	evaluation	1015:1024	arg1	panel					1026:1030	a sensory evaluation panel	1005:1030	a sensory evaluation panel	1005:1030	However, mouthfeel (viscosity or weight) of wines with or without added pectin as determined by a sensory evaluation panel was not altered by the presence of these yeasts.					
36529878	7	5	theme	Significant	1081:1091	arg1	interactions					1093:1104	Significant interactions	1081:1104	Significant interactions among the yeast utilized, pectin addition, and 6-month aging	1081:1165	Significant interactions among the yeast utilized, pectin addition, and 6-month aging affected some flavors (solvent) of Merlot, while addition of NSY increased other attributes (cherry) during aging.					
36529878	7	6	theme	NSY	1228:1230	arg1	addition					1216:1223	addition	1216:1223	addition of NSY	1216:1230	Significant interactions among the yeast utilized, pectin addition, and 6-month aging affected some flavors (solvent) of Merlot, while addition of NSY increased other attributes (cherry) during aging.					
36529878	4	7	theme	species	690:696	arg1	mixture					698:704	a three species mixture	682:704	(b) a three species mixture of NSY	678:711	Apple pectin (0 or 0.5 g/L) was added to Merlot and Chardonnay grape musts after inoculation with (a) only Saccharomyces cerevisiae or (b) a three species mixture of NSY; after 3 days, S. cerevisiae was added.					
36529878	8	8	theme	sensory	1285:1291	arg1	differences					1293:1303	No sensory differences	1282:1303	No sensory differences	1282:1303	No sensory differences were perceived among Chardonnay samples due to NSY; however, aging from 6 to 18 months increased the intensity of 40 sensory attributes.					
36529878	11	9	theme	Merlot	1860:1865	arg1	wines					1867:1871	Merlot wines	1860:1871	Merlot wines made with NSY, which may increase perceived quality	1860:1923	After one additional year of aging, an increase in cherry flavor was observed in Merlot wines made with NSY, which may increase perceived quality.					
36529878	8	10	from	months	1385:1390	arg1	aging					1366:1370	aging	1366:1370	aging from 6 to 18 months	1366:1390	No sensory differences were perceived among Chardonnay samples due to NSY; however, aging from 6 to 18 months increased the intensity of 40 sensory attributes.					
36529878	2	11	theme	sensory	288:294	arg1	quality					296:302	sensory quality	288:302	sensory quality of the wine	288:314	However, research exploring the influence on sensory quality of the wine, particularly during storage, is limited.					
36529878	7	12	theme	6-month	1153:1159	arg1	aging					1161:1165	6-month aging	1153:1165	6-month aging	1153:1165	Significant interactions among the yeast utilized, pectin addition, and 6-month aging affected some flavors (solvent) of Merlot, while addition of NSY increased other attributes (cherry) during aging.					
36529878	6	13	dep	mouthfeel	918:926	arg1	weight					942:947	weight	942:947	weight	942:947	However, mouthfeel (viscosity or weight) of wines with or without added pectin as determined by a sensory evaluation panel was not altered by the presence of these yeasts.					
36529878	6	13	dep	mouthfeel	918:926	arg1	viscosity					929:937	viscosity	929:937	viscosity	929:937	However, mouthfeel (viscosity or weight) of wines with or without added pectin as determined by a sensory evaluation panel was not altered by the presence of these yeasts.					
36529878	6	14	theme	added	975:979	arg1	pectin					981:986	added pectin	975:986	added pectin as determined by a sensory evaluation panel	975:1030	However, mouthfeel (viscosity or weight) of wines with or without added pectin as determined by a sensory evaluation panel was not altered by the presence of these yeasts.					
36529878	1	15	dep	use	121:123	arg1	the					117:119	the	117:119	the	117:119	Recently, the use and commercial availability of non-Saccharomyces yeasts (NSY) in winemaking to reduce alcohol content have increased.					
36529878	5	16	theme	acid	839:842	arg1	concentrations					806:819	higher concentrations	799:819	higher concentrations of d-galacturonic acid and glycerol concentration	799:869	Addition of NSY with added pectin resulted in higher concentrations of d-galacturonic acid and glycerol concentration in the wines after 6 months of aging.					
36529878	3	17	theme	sensory	421:427	arg1	profiles					429:436	the sensory profiles	417:436	the sensory profiles of Merlot and Chardonnay wines made with pectinase-producing NSY	417:501	Therefore, the objective of this study was to characterize the sensory profiles of Merlot and Chardonnay wines made with pectinase-producing NSY, with added substrate, that is, pectin.					
36529878	0	18	theme	wines	45:49	arg1	properties					8:17	Sensory properties	0:17	Sensory properties of 6- and 18-month-stored wines	0:49	Sensory properties of 6- and 18-month-stored wines made with pectinase-producing non-Saccharomyces yeasts.					
36529878	10	19	theme	wine	1773:1776	arg1	composition					1742:1752	the chemical composition	1729:1752	the chemical composition of the final young wine	1729:1776	PRACTICAL APPLICATION: We found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY) modified the chemical composition of the final young wine.					
36529878	6	20	theme	sensory	1007:1013	arg1	panel					1026:1030	a sensory evaluation panel	1005:1030	a sensory evaluation panel	1005:1030	However, mouthfeel (viscosity or weight) of wines with or without added pectin as determined by a sensory evaluation panel was not altered by the presence of these yeasts.					
36529878	11	21	theme	cherry	1830:1835	arg1	flavor					1837:1842	cherry flavor	1830:1842	cherry flavor	1830:1842	After one additional year of aging, an increase in cherry flavor was observed in Merlot wines made with NSY, which may increase perceived quality.					
36529878	10	22	theme	chemical	1733:1740	arg1	composition					1742:1752	the chemical composition	1729:1752	the chemical composition of the final young wine	1729:1776	PRACTICAL APPLICATION: We found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY) modified the chemical composition of the final young wine.					
36529878	4	23	theme	grape	606:610	arg1	musts					612:616	grape musts	606:616	grape musts	606:616	Apple pectin (0 or 0.5 g/L) was added to Merlot and Chardonnay grape musts after inoculation with (a) only Saccharomyces cerevisiae or (b) a three species mixture of NSY; after 3 days, S. cerevisiae was added.					
36529878	10	24	theme	young	1767:1771	arg1	wine					1773:1776	the final young wine	1757:1776	the final young wine	1757:1776	PRACTICAL APPLICATION: We found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY) modified the chemical composition of the final young wine.					
36529878	10	25	theme	PRACTICAL	1612:1620	arg1	APPLICATION					1622:1632	PRACTICAL APPLICATION	1612:1632	PRACTICAL APPLICATION: We found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY)	1612:1718	PRACTICAL APPLICATION: We found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY) modified the chemical composition of the final young wine.					
36529878	5	26	theme	glycerol	848:855	arg1	concentration					857:869	glycerol concentration	848:869	glycerol concentration	848:869	Addition of NSY with added pectin resulted in higher concentrations of d-galacturonic acid and glycerol concentration in the wines after 6 months of aging.					
36529878	4	27	dep	mixture	698:704	arg1	b					679:679	b	679:679	b	679:679	Apple pectin (0 or 0.5 g/L) was added to Merlot and Chardonnay grape musts after inoculation with (a) only Saccharomyces cerevisiae or (b) a three species mixture of NSY; after 3 days, S. cerevisiae was added.					
36529878	5	28	theme	added	774:778	arg1	pectin					780:785	added pectin	774:785	added pectin	774:785	Addition of NSY with added pectin resulted in higher concentrations of d-galacturonic acid and glycerol concentration in the wines after 6 months of aging.					
36529878	0	29	theme	Sensory	0:6	arg1	properties					8:17	Sensory properties	0:17	Sensory properties of 6- and 18-month-stored wines	0:49	Sensory properties of 6- and 18-month-stored wines made with pectinase-producing non-Saccharomyces yeasts.					
36529878	9	30	theme	useful	1522:1527	arg1	tool					1529:1532	a useful tool	1520:1532	a useful tool to alter wine quality in Merlot by increasing specific aromas during storage	1520:1609	Though mouthfeel was not specifically affected, the utilization of NSY may be a useful tool to alter wine quality in Merlot by increasing specific aromas during storage.					
36529878	9	30	theme	useful	1522:1527	arg1	utilization					1494:1504	the utilization	1490:1504	the utilization of NSY	1490:1511	Though mouthfeel was not specifically affected, the utilization of NSY may be a useful tool to alter wine quality in Merlot by increasing specific aromas during storage.					
36529878	2	31	theme	wine	311:314	arg1	quality					296:302	sensory quality	288:302	sensory quality of the wine	288:314	However, research exploring the influence on sensory quality of the wine, particularly during storage, is limited.					
36529878	8	32	theme	Chardonnay	1326:1335	arg1	samples					1337:1343	Chardonnay samples	1326:1343	Chardonnay samples due to NSY	1326:1354	No sensory differences were perceived among Chardonnay samples due to NSY; however, aging from 6 to 18 months increased the intensity of 40 sensory attributes.					
36529878	11	33	from	increase	1818:1825	arg1	flavor					1837:1842	cherry flavor	1830:1842	cherry flavor	1830:1842	After one additional year of aging, an increase in cherry flavor was observed in Merlot wines made with NSY, which may increase perceived quality.					
36529878	5	34	with	Addition	753:760	arg1	pectin					780:785	added pectin	774:785	added pectin	774:785	Addition of NSY with added pectin resulted in higher concentrations of d-galacturonic acid and glycerol concentration in the wines after 6 months of aging.					
36529878	11	35	theme	additional	1789:1798	arg1	year					1800:1803	one additional year	1785:1803	one additional year of aging	1785:1812	After one additional year of aging, an increase in cherry flavor was observed in Merlot wines made with NSY, which may increase perceived quality.					
36529878	6	36	theme	wines	953:957	arg1	mouthfeel					918:926	mouthfeel	918:926	mouthfeel (viscosity or weight) of wines with or without added pectin as determined by a sensory evaluation panel	918:1030	However, mouthfeel (viscosity or weight) of wines with or without added pectin as determined by a sensory evaluation panel was not altered by the presence of these yeasts.					
36529878	4	37	dep	cerevisiae	664:673	arg1	a					642:642	a	642:642	a	642:642	Apple pectin (0 or 0.5 g/L) was added to Merlot and Chardonnay grape musts after inoculation with (a) only Saccharomyces cerevisiae or (b) a three species mixture of NSY; after 3 days, S. cerevisiae was added.					
36529878	4	38	theme	Apple	543:547	arg1	pectin					549:554	Apple pectin	543:554	Apple pectin (0 or 0.5 g/L)	543:569	Apple pectin (0 or 0.5 g/L) was added to Merlot and Chardonnay grape musts after inoculation with (a) only Saccharomyces cerevisiae or (b) a three species mixture of NSY; after 3 days, S. cerevisiae was added.					
36529878	5	39	theme	concentration	857:869	arg1	concentrations					806:819	higher concentrations	799:819	higher concentrations of d-galacturonic acid and glycerol concentration	799:869	Addition of NSY with added pectin resulted in higher concentrations of d-galacturonic acid and glycerol concentration in the wines after 6 months of aging.					
36529878	4	40	with	inoculation	624:634	arg1	cerevisiae					664:673	Saccharomyces cerevisiae	650:673	(a) only Saccharomyces cerevisiae	641:673	Apple pectin (0 or 0.5 g/L) was added to Merlot and Chardonnay grape musts after inoculation with (a) only Saccharomyces cerevisiae or (b) a three species mixture of NSY; after 3 days, S. cerevisiae was added.					
36529878	4	40	with	inoculation	624:634	arg1	mixture					698:704	a three species mixture	682:704	(b) a three species mixture of NSY	678:711	Apple pectin (0 or 0.5 g/L) was added to Merlot and Chardonnay grape musts after inoculation with (a) only Saccharomyces cerevisiae or (b) a three species mixture of NSY; after 3 days, S. cerevisiae was added.					
36529878	7	41	theme	pectin	1132:1137	arg1	addition					1139:1146	pectin addition	1132:1146	pectin addition	1132:1146	Significant interactions among the yeast utilized, pectin addition, and 6-month aging affected some flavors (solvent) of Merlot, while addition of NSY increased other attributes (cherry) during aging.					
36529878	12	42	theme	NSY	1969:1971	arg1	use					1936:1938	the use	1932:1938	the use of these pectinase-producing NSY	1932:1971	Thus, the use of these pectinase-producing NSY may be a useful tool for winemakers.					
36529878	12	42	theme	NSY	1969:1971	arg1	tool					1989:1992	a useful tool	1980:1992	a useful tool for winemakers	1980:2007	Thus, the use of these pectinase-producing NSY may be a useful tool for winemakers.					
36529878	3	43	theme	Chardonnay	452:461	arg1	wines					463:467	Merlot and Chardonnay wines	441:467	Merlot and Chardonnay wines made with pectinase-producing NSY	441:501	Therefore, the objective of this study was to characterize the sensory profiles of Merlot and Chardonnay wines made with pectinase-producing NSY, with added substrate, that is, pectin.					
36529878	5	44	theme	d-galacturonic	824:837	arg1	acid					839:842	d-galacturonic acid	824:842	d-galacturonic acid	824:842	Addition of NSY with added pectin resulted in higher concentrations of d-galacturonic acid and glycerol concentration in the wines after 6 months of aging.					
36529878	12	45	theme	useful	1982:1987	arg1	use					1936:1938	the use	1932:1938	the use of these pectinase-producing NSY	1932:1971	Thus, the use of these pectinase-producing NSY may be a useful tool for winemakers.					
36529878	12	45	theme	useful	1982:1987	arg1	tool					1989:1992	a useful tool	1980:1992	a useful tool for winemakers	1980:2007	Thus, the use of these pectinase-producing NSY may be a useful tool for winemakers.					
36529878	1	46	theme	alcohol	211:217	arg1	content					219:225	alcohol content	211:225	alcohol content	211:225	Recently, the use and commercial availability of non-Saccharomyces yeasts (NSY) in winemaking to reduce alcohol content have increased.					
36529878	5	47	theme	NSY	765:767	arg1	Addition					753:760	Addition	753:760	Addition of NSY with added pectin	753:785	Addition of NSY with added pectin resulted in higher concentrations of d-galacturonic acid and glycerol concentration in the wines after 6 months of aging.					
36529878	9	48	theme	wine	1543:1546	arg1	quality					1548:1554	wine quality	1543:1554	wine quality in Merlot	1543:1564	Though mouthfeel was not specifically affected, the utilization of NSY may be a useful tool to alter wine quality in Merlot by increasing specific aromas during storage.					
36529878	7	49	theme	Merlot	1202:1207	arg1	solvent					1190:1196	solvent	1190:1196	solvent	1190:1196	Significant interactions among the yeast utilized, pectin addition, and 6-month aging affected some flavors (solvent) of Merlot, while addition of NSY increased other attributes (cherry) during aging.					
36529878	7	49	theme	Merlot	1202:1207	arg1	flavors					1181:1187	some flavors	1176:1187	some flavors (solvent) of Merlot	1176:1207	Significant interactions among the yeast utilized, pectin addition, and 6-month aging affected some flavors (solvent) of Merlot, while addition of NSY increased other attributes (cherry) during aging.					
36529878	6	50	dep	pectin	981:986	arg1	determined					991:1000	determined	991:1000	determined by a sensory evaluation panel	991:1030	However, mouthfeel (viscosity or weight) of wines with or without added pectin as determined by a sensory evaluation panel was not altered by the presence of these yeasts.					
36529878	3	51	theme	Merlot	441:446	arg1	wines					463:467	Merlot and Chardonnay wines	441:467	Merlot and Chardonnay wines made with pectinase-producing NSY	441:501	Therefore, the objective of this study was to characterize the sensory profiles of Merlot and Chardonnay wines made with pectinase-producing NSY, with added substrate, that is, pectin.					
36529878	11	52	located	observed	1848:1855	arg2	increase					1818:1825	an increase	1815:1825	an increase in cherry flavor	1815:1842	After one additional year of aging, an increase in cherry flavor was observed in Merlot wines made with NSY, which may increase perceived quality.					
36529878	11	52	located	observed	1848:1855	arg1	wines					1867:1871	Merlot wines	1860:1871	Merlot wines made with NSY, which may increase perceived quality	1860:1923	After one additional year of aging, an increase in cherry flavor was observed in Merlot wines made with NSY, which may increase perceived quality.					
36529878	4	53	dep	pectin	549:554	arg1	0					557:557	0	557:557	0	557:557	Apple pectin (0 or 0.5 g/L) was added to Merlot and Chardonnay grape musts after inoculation with (a) only Saccharomyces cerevisiae or (b) a three species mixture of NSY; after 3 days, S. cerevisiae was added.					
36529878	4	53	dep	pectin	549:554	arg1	0.5 g/L					562:568	0.5 g/L	562:568	0.5 g/L	562:568	Apple pectin (0 or 0.5 g/L) was added to Merlot and Chardonnay grape musts after inoculation with (a) only Saccharomyces cerevisiae or (b) a three species mixture of NSY; after 3 days, S. cerevisiae was added.					
36529878	4	54	dep	Merlot	584:589	arg1	musts					612:616	grape musts	606:616	grape musts	606:616	Apple pectin (0 or 0.5 g/L) was added to Merlot and Chardonnay grape musts after inoculation with (a) only Saccharomyces cerevisiae or (b) a three species mixture of NSY; after 3 days, S. cerevisiae was added.					
36529878	10	55	dep	APPLICATION	1622:1632	arg1	found					1638:1642	found	1638:1642	found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY)	1638:1718	PRACTICAL APPLICATION: We found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY) modified the chemical composition of the final young wine.					
36529878	3	56	theme	study	391:395	arg1	objective					373:381	the objective	369:381	the objective of this study	369:395	Therefore, the objective of this study was to characterize the sensory profiles of Merlot and Chardonnay wines made with pectinase-producing NSY, with added substrate, that is, pectin.					
36529878	8	57	theme	attributes	1430:1439	arg1	intensity					1406:1414	the intensity	1402:1414	the intensity of 40 sensory attributes	1402:1439	No sensory differences were perceived among Chardonnay samples due to NSY; however, aging from 6 to 18 months increased the intensity of 40 sensory attributes.					
36529878	10	58	theme	final	1761:1765	arg1	wine					1773:1776	the final young wine	1757:1776	the final young wine	1757:1776	PRACTICAL APPLICATION: We found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY) modified the chemical composition of the final young wine.					
36529878	8	59	theme	due	1345:1347	arg1	samples					1337:1343	Chardonnay samples	1326:1343	Chardonnay samples due to NSY	1326:1354	No sensory differences were perceived among Chardonnay samples due to NSY; however, aging from 6 to 18 months increased the intensity of 40 sensory attributes.					
36529878	7	60	theme	other	1242:1246	arg1	cherry					1260:1265	cherry	1260:1265	cherry	1260:1265	Significant interactions among the yeast utilized, pectin addition, and 6-month aging affected some flavors (solvent) of Merlot, while addition of NSY increased other attributes (cherry) during aging.					
36529878	7	60	theme	other	1242:1246	arg1	attributes					1248:1257	other attributes	1242:1257	other attributes (cherry)	1242:1266	Significant interactions among the yeast utilized, pectin addition, and 6-month aging affected some flavors (solvent) of Merlot, while addition of NSY increased other attributes (cherry) during aging.					
36529878	9	61	theme	NSY	1509:1511	arg1	tool					1529:1532	a useful tool	1520:1532	a useful tool to alter wine quality in Merlot by increasing specific aromas during storage	1520:1609	Though mouthfeel was not specifically affected, the utilization of NSY may be a useful tool to alter wine quality in Merlot by increasing specific aromas during storage.					
36529878	9	61	theme	NSY	1509:1511	arg1	utilization					1494:1504	the utilization	1490:1504	the utilization of NSY	1490:1511	Though mouthfeel was not specifically affected, the utilization of NSY may be a useful tool to alter wine quality in Merlot by increasing specific aromas during storage.					
36529878	3	62	theme	pectinase-producing	479:497	arg1	NSY					499:501	pectinase-producing NSY	479:501	pectinase-producing NSY	479:501	Therefore, the objective of this study was to characterize the sensory profiles of Merlot and Chardonnay wines made with pectinase-producing NSY, with added substrate, that is, pectin.					
36529878	8	63	theme	sensory	1422:1428	arg1	attributes					1430:1439	40 sensory attributes	1419:1439	40 sensory attributes	1419:1439	No sensory differences were perceived among Chardonnay samples due to NSY; however, aging from 6 to 18 months increased the intensity of 40 sensory attributes.					
36529878	10	64	theme	pectinase-producing	1669:1687	arg1	NSY					1715:1717	NSY	1715:1717	NSY	1715:1717	PRACTICAL APPLICATION: We found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY) modified the chemical composition of the final young wine.					
36529878	10	64	theme	pectinase-producing	1669:1687	arg1	yeasts					1707:1712	pectinase-producing non-Saccharomyces yeasts	1669:1712	pectinase-producing non-Saccharomyces yeasts (NSY)	1669:1718	PRACTICAL APPLICATION: We found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY) modified the chemical composition of the final young wine.					
36529878	5	65	theme	aging	902:906	arg1	6 months					890:897	6 months	890:897	6 months of aging	890:906	Addition of NSY with added pectin resulted in higher concentrations of d-galacturonic acid and glycerol concentration in the wines after 6 months of aging.					
36529878	1	66	theme	commercial	129:138	arg1	availability					140:151	commercial availability	129:151	commercial availability	129:151	Recently, the use and commercial availability of non-Saccharomyces yeasts (NSY) in winemaking to reduce alcohol content have increased.					
36529878	2	67	from	influence	275:283	arg1	quality					296:302	sensory quality	288:302	sensory quality of the wine	288:314	However, research exploring the influence on sensory quality of the wine, particularly during storage, is limited.					
36529878	8	68	dep	18	1382:1383	arg1	to					1379:1380	to	1379:1380	to	1379:1380	No sensory differences were perceived among Chardonnay samples due to NSY; however, aging from 6 to 18 months increased the intensity of 40 sensory attributes.					
36529878	12	69	theme	pectinase-producing	1949:1967	arg1	NSY					1969:1971	these pectinase-producing NSY	1943:1971	these pectinase-producing NSY	1943:1971	Thus, the use of these pectinase-producing NSY may be a useful tool for winemakers.					
36529878	4	70	theme	NSY	709:711	arg1	mixture					698:704	a three species mixture	682:704	(b) a three species mixture of NSY	678:711	Apple pectin (0 or 0.5 g/L) was added to Merlot and Chardonnay grape musts after inoculation with (a) only Saccharomyces cerevisiae or (b) a three species mixture of NSY; after 3 days, S. cerevisiae was added.					
36529878	1	71	theme	non-Saccharomyces	156:172	arg1	NSY					182:184	NSY	182:184	NSY	182:184	Recently, the use and commercial availability of non-Saccharomyces yeasts (NSY) in winemaking to reduce alcohol content have increased.					
36529878	1	71	theme	non-Saccharomyces	156:172	arg1	yeasts					174:179	non-Saccharomyces yeasts	156:179	non-Saccharomyces yeasts (NSY)	156:185	Recently, the use and commercial availability of non-Saccharomyces yeasts (NSY) in winemaking to reduce alcohol content have increased.					
36529878	3	72	theme	wines	463:467	arg1	profiles					429:436	the sensory profiles	417:436	the sensory profiles of Merlot and Chardonnay wines made with pectinase-producing NSY	417:501	Therefore, the objective of this study was to characterize the sensory profiles of Merlot and Chardonnay wines made with pectinase-producing NSY, with added substrate, that is, pectin.					
36529878	0	73	theme	pectinase-producing	61:79	arg1	yeasts					99:104	pectinase-producing non-Saccharomyces yeasts	61:104	pectinase-producing non-Saccharomyces yeasts	61:104	Sensory properties of 6- and 18-month-stored wines made with pectinase-producing non-Saccharomyces yeasts.					
36529878	11	74	theme	aging	1808:1812	arg1	year					1800:1803	one additional year	1785:1803	one additional year of aging	1785:1812	After one additional year of aging, an increase in cherry flavor was observed in Merlot wines made with NSY, which may increase perceived quality.					
36529878	11	75	theme	perceived	1907:1915	arg1	quality					1917:1923	perceived quality	1907:1923	perceived quality	1907:1923	After one additional year of aging, an increase in cherry flavor was observed in Merlot wines made with NSY, which may increase perceived quality.					
36529878	9	76	from	quality	1548:1554	arg1	Merlot					1559:1564	Merlot	1559:1564	Merlot	1559:1564	Though mouthfeel was not specifically affected, the utilization of NSY may be a useful tool to alter wine quality in Merlot by increasing specific aromas during storage.					
36529878	9	77	theme	specific	1580:1587	arg1	aromas					1589:1594	specific aromas	1580:1594	specific aromas	1580:1594	Though mouthfeel was not specifically affected, the utilization of NSY may be a useful tool to alter wine quality in Merlot by increasing specific aromas during storage.					
36529878	10	78	theme	non-Saccharomyces	1689:1705	arg1	NSY					1715:1717	NSY	1715:1717	NSY	1715:1717	PRACTICAL APPLICATION: We found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY) modified the chemical composition of the final young wine.					
36529878	10	78	theme	non-Saccharomyces	1689:1705	arg1	yeasts					1707:1712	pectinase-producing non-Saccharomyces yeasts	1669:1712	pectinase-producing non-Saccharomyces yeasts (NSY)	1669:1718	PRACTICAL APPLICATION: We found that must fermented with pectinase-producing non-Saccharomyces yeasts (NSY) modified the chemical composition of the final young wine.					
36529878	6	79	theme	yeasts	1073:1078	arg1	presence					1055:1062	the presence	1051:1062	the presence of these yeasts	1051:1078	However, mouthfeel (viscosity or weight) of wines with or without added pectin as determined by a sensory evaluation panel was not altered by the presence of these yeasts.					
36529878	3	80	theme	added	509:513	arg1	substrate					515:523	added substrate	509:523	added substrate	509:523	Therefore, the objective of this study was to characterize the sensory profiles of Merlot and Chardonnay wines made with pectinase-producing NSY, with added substrate, that is, pectin.					
35714730	3	0	theme	distinct	449:456	arg1	effects					479:485	distinct from its weight loss effects	449:485	distinct from its weight loss effects	449:485	Previous work has shown that 10,12 CLA is atheroprotective in mice by a mechanism that may be distinct from its weight loss effects, but this exact mechanism is unclear.					
35714730	3	0	theme	distinct	449:456	arg1	mechanism					427:435	a mechanism	425:435	a mechanism that may be distinct from its weight loss effects	425:485	Previous work has shown that 10,12 CLA is atheroprotective in mice by a mechanism that may be distinct from its weight loss effects, but this exact mechanism is unclear.					
35714730	2	1	theme	10,12-conjugated	262:277	arg1	foods					255:259	ruminant animal foods	239:259	ruminant animal foods	239:259	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	2	1	theme	10,12-conjugated	262:277	arg1	acid					288:291	10,12-conjugated linoleic acid	262:291	10,12-conjugated linoleic acid (10,12 CLA)	262:303	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	2	1	theme	10,12-conjugated	262:277	arg1	CLA					300:302	10,12 CLA	294:302	10,12 CLA	294:302	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	5	2	from	high-sucrose-HDL	906:921	arg1	adipocytes					935:944	cultured adipocytes	926:944	cultured adipocytes	926:944	We show that 10,12 CLA-HDL exerted a stronger anti-inflammatory effect than CR- or high-fat high-sucrose-HDL in cultured adipocytes.					
35714730	2	3	theme	foods	255:259	arg1	component					226:234	One dietary component	214:234	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA),	214:304	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	4	4	dep	CR	710:711	arg1	group					737:741	weight-matched control group	714:741	CR; weight-matched control group	710:741	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	4	5	theme	obese	754:758	arg1	group					768:772	an obese control group	751:772	an obese control group consuming a high-fat high-sucrose diet	751:811	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	4	6	theme	LDL	597:599	arg1	receptor					601:608	LDL receptor	597:608	obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group)	591:742	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	9	7	theme	antiatherosclerotic	1644:1662	arg1	protein					1679:1685	an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein	1610:1685	an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein	1610:1685	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	9	7	theme	antiatherosclerotic	1644:1662	arg1	alpha-1-antitrypsin					1589:1607	alpha-1-antitrypsin	1589:1607	alpha-1-antitrypsin	1589:1607	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	6	8	theme	large-sized	1052:1062	arg1	HDL-Ps					1064:1069	more medium- and large-sized HDL-Ps	1035:1069	more medium- and large-sized HDL-Ps	1035:1069	Furthermore, the 10,12 CLA-HDL particle (HDL-P) concentration was higher, attributed to more medium- and large-sized HDL-Ps.					
35714730	4	9	theme	HDL	559:561	arg1	composition					563:573	HDL composition	559:573	HDL composition	559:573	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	9	10	with	consistent	1502:1511	arg1	concentration					1536:1548	a higher particle concentration	1518:1548	a higher particle concentration	1518:1548	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	10	11	theme	its atheroprotective	1735:1754	arg1	effects					1756:1762	its atheroprotective effects	1735:1762	its atheroprotective effects	1735:1762	We conclude that 10,12 CLA may therefore exert its atheroprotective effects by increasing HDL-P concentration, HDL anti-inflammatory potential, and promoting beneficial effects on cholesterol efflux.					
35714730	1	12	theme	risk	192:195	arg1	inflammation					121:132	inflammation	121:132	inflammation	121:132	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	1	12	theme	risk	192:195	arg1	resistance					143:152	insulin resistance	135:152	insulin resistance	135:152	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	1	12	theme	risk	192:195	arg1	factors					197:203	major risk factors	186:203	major risk factors for CVD	186:211	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	1	12	theme	risk	192:195	arg1	diabetes					166:173	type 2 diabetes	159:173	type 2 diabetes	159:173	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	4	13	theme	high-sucrose	795:806	arg1	diet					808:811	a high-fat high-sucrose diet	784:811	a high-fat high-sucrose diet	784:811	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	8	14	theme	cholesterol	1375:1385	arg1	efflux					1387:1392	cholesterol efflux	1375:1392	increased cholesterol efflux potential of these cells	1365:1417	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	10	15	theme	HDL-P	1778:1782	arg1	concentration					1784:1796	HDL-P concentration	1778:1796	HDL-P concentration	1778:1796	We conclude that 10,12 CLA may therefore exert its atheroprotective effects by increasing HDL-P concentration, HDL anti-inflammatory potential, and promoting beneficial effects on cholesterol efflux.					
35714730	4	16	theme	losing	635:640	arg1	weight					642:647	losing weight	635:647	losing weight	635:647	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	8	17	theme	increased	1288:1296	arg1	expression					1298:1307	increased expression	1288:1307	increased expression of cholesterol transporters Abca1 and Abcg1	1288:1351	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	2	18	theme	ruminant	239:246	arg1	foods					255:259	ruminant animal foods	239:259	ruminant animal foods	239:259	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	2	18	theme	ruminant	239:246	arg1	acid					288:291	10,12-conjugated linoleic acid	262:291	10,12-conjugated linoleic acid (10,12 CLA)	262:303	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	5	19	theme	10,12	827:831	arg1	CLA-HDL					833:839	10,12 CLA-HDL	827:839	10,12 CLA-HDL	827:839	We show that 10,12 CLA-HDL exerted a stronger anti-inflammatory effect than CR- or high-fat high-sucrose-HDL in cultured adipocytes.					
35714730	7	20	theme	cholesterol	1080:1090	arg1	efflux					1092:1097	Passive cholesterol efflux	1072:1097	Passive cholesterol efflux capacity of 10,12 CLA-HDL	1072:1123	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	8	21	theme	Murine	1227:1232	arg1	macrophages					1234:1244	Murine macrophages	1227:1244	Murine macrophages treated with 10,12 CLA in vitro	1227:1276	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	3	22	from	loss	474:477	arg1	distinct					449:456	distinct	449:456	distinct	449:456	Previous work has shown that 10,12 CLA is atheroprotective in mice by a mechanism that may be distinct from its weight loss effects, but this exact mechanism is unclear.					
35714730	9	23	theme	Apoa1	1467:1471	arg1	content					1473:1479	elevated Apoa1 content	1458:1479	elevated Apoa1 content in 10,12 CLA-HDL-Ps	1458:1499	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	9	24	from	content	1473:1479	arg1	CLA-HDL-Ps					1490:1499	10,12 CLA-HDL-Ps	1484:1499	10,12 CLA-HDL-Ps	1484:1499	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	10	25	from	concentration	1784:1796	arg1	efflux					1880:1885	cholesterol efflux	1868:1885	cholesterol efflux	1868:1885	We conclude that 10,12 CLA may therefore exert its atheroprotective effects by increasing HDL-P concentration, HDL anti-inflammatory potential, and promoting beneficial effects on cholesterol efflux.					
35714730	9	26	theme	proteomics	1429:1438	arg1	analysis					1440:1447	proteomics analysis	1429:1447	proteomics analysis	1429:1447	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	0	27	from	composition	60:70	arg1	mice					88:91	mice	88:91	mice	88:91	10,12-Conjugated linoleic acid supplementation improves HDL composition and function in mice.					
35714730	8	28	theme	10,12	1259:1263	arg1	in vitro					1269:1276	10,12 CLA in vitro	1259:1276	10,12 CLA in vitro	1259:1276	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	7	29	theme	10,12	1111:1115	arg1	CLA-HDL					1117:1123	10,12 CLA-HDL	1111:1123	10,12 CLA-HDL	1111:1123	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	5	30	theme	CR-	890:892	arg1	high-sucrose-HDL					906:921	CR- or high-fat high-sucrose-HDL	890:921	CR- or high-fat high-sucrose-HDL in cultured adipocytes	890:944	We show that 10,12 CLA-HDL exerted a stronger anti-inflammatory effect than CR- or high-fat high-sucrose-HDL in cultured adipocytes.					
35714730	9	31	theme	anti-inflammatory	1622:1638	arg1	protein					1679:1685	an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein	1610:1685	an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein	1610:1685	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	9	31	theme	anti-inflammatory	1622:1638	arg1	alpha-1-antitrypsin					1589:1607	alpha-1-antitrypsin	1589:1607	alpha-1-antitrypsin	1589:1607	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	0	32	theme	10,12-Conjugated	0:15	arg1	supplementation					31:45	10,12-Conjugated linoleic acid supplementation	0:45	10,12-Conjugated linoleic acid supplementation	0:45	10,12-Conjugated linoleic acid supplementation improves HDL composition and function in mice.					
35714730	10	33	from	potential	1821:1829	arg1	efflux					1880:1885	cholesterol efflux	1868:1885	cholesterol efflux	1868:1885	We conclude that 10,12 CLA may therefore exert its atheroprotective effects by increasing HDL-P concentration, HDL anti-inflammatory potential, and promoting beneficial effects on cholesterol efflux.					
35714730	6	34	theme	particle	978:985	arg1	higher					1013:1018	higher	1013:1018	higher	1013:1018	Furthermore, the 10,12 CLA-HDL particle (HDL-P) concentration was higher, attributed to more medium- and large-sized HDL-Ps.					
35714730	6	34	theme	particle	978:985	arg1	concentration					995:1007	the 10,12 CLA-HDL particle (HDL-P) concentration	960:1007	the 10,12 CLA-HDL particle (HDL-P) concentration	960:1007	Furthermore, the 10,12 CLA-HDL particle (HDL-P) concentration was higher, attributed to more medium- and large-sized HDL-Ps.					
35714730	4	35	theme	10,12	660:664	arg1	supplementation					670:684	10,12 CLA supplementation	660:684	10,12 CLA supplementation	660:684	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	9	36	theme	higher	1520:1525	arg1	concentration					1536:1548	a higher particle concentration	1518:1548	a higher particle concentration	1518:1548	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	0	37	theme	acid	26:29	arg1	supplementation					31:45	10,12-Conjugated linoleic acid supplementation	0:45	10,12-Conjugated linoleic acid supplementation	0:45	10,12-Conjugated linoleic acid supplementation improves HDL composition and function in mice.					
35714730	5	38	theme	stronger	851:858	arg1	effect					878:883	a stronger anti-inflammatory effect	849:883	a stronger anti-inflammatory effect	849:883	We show that 10,12 CLA-HDL exerted a stronger anti-inflammatory effect than CR- or high-fat high-sucrose-HDL in cultured adipocytes.					
35714730	6	39	theme	10,12	964:968	arg1	HDL-P					988:992	HDL-P	988:992	HDL-P	988:992	Furthermore, the 10,12 CLA-HDL particle (HDL-P) concentration was higher, attributed to more medium- and large-sized HDL-Ps.					
35714730	6	39	theme	10,12	964:968	arg1	particle					978:985	10,12 CLA-HDL particle	964:985	the 10,12 CLA-HDL particle (HDL-P) concentration	960:1007	Furthermore, the 10,12 CLA-HDL particle (HDL-P) concentration was higher, attributed to more medium- and large-sized HDL-Ps.					
35714730	4	40	theme	control	729:735	arg1	group					737:741	weight-matched control group	714:741	CR; weight-matched control group	710:741	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	9	41	theme	particle	1527:1534	arg1	concentration					1536:1548	a higher particle concentration	1518:1548	a higher particle concentration	1518:1548	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	7	42	from	type	1200:1203	arg1	arch					1221:1224	the aortic arch	1210:1224	the aortic arch	1210:1224	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	3	43	theme	Previous	355:362	arg1	work					364:367	Previous work	355:367	Previous work	355:367	Previous work has shown that 10,12 CLA is atheroprotective in mice by a mechanism that may be distinct from its weight loss effects, but this exact mechanism is unclear.					
35714730	10	44	theme	promoting	1836:1844	arg1	effects					1857:1863	promoting beneficial effects	1836:1863	promoting beneficial effects on cholesterol efflux	1836:1885	We conclude that 10,12 CLA may therefore exert its atheroprotective effects by increasing HDL-P concentration, HDL anti-inflammatory potential, and promoting beneficial effects on cholesterol efflux.					
35714730	8	45	theme	transporters	1324:1335	arg1	expression					1298:1307	increased expression	1288:1307	increased expression of cholesterol transporters Abca1 and Abcg1	1288:1351	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	7	46	theme	B	1198:1198	arg1	type					1200:1203	HDL receptor scavenger receptor class B type 1	1160:1205	HDL receptor scavenger receptor class B type 1 in the aortic arch	1160:1224	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	10	47	theme	anti-inflammatory	1803:1819	arg1	potential					1821:1829	HDL anti-inflammatory potential	1799:1829	HDL anti-inflammatory potential	1799:1829	We conclude that 10,12 CLA may therefore exert its atheroprotective effects by increasing HDL-P concentration, HDL anti-inflammatory potential, and promoting beneficial effects on cholesterol efflux.					
35714730	5	48	theme	high-fat	897:904	arg1	high-sucrose-HDL					906:921	CR- or high-fat high-sucrose-HDL	890:921	CR- or high-fat high-sucrose-HDL in cultured adipocytes	890:944	We show that 10,12 CLA-HDL exerted a stronger anti-inflammatory effect than CR- or high-fat high-sucrose-HDL in cultured adipocytes.					
35714730	7	49	theme	receptor	1183:1190	arg1	type					1200:1203	HDL receptor scavenger receptor class B type 1	1160:1205	HDL receptor scavenger receptor class B type 1 in the aortic arch	1160:1224	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	10	50	theme	cholesterol	1868:1878	arg1	efflux					1880:1885	cholesterol efflux	1868:1885	cholesterol efflux	1868:1885	We conclude that 10,12 CLA may therefore exert its atheroprotective effects by increasing HDL-P concentration, HDL anti-inflammatory potential, and promoting beneficial effects on cholesterol efflux.					
35714730	2	51	theme	weight	332:337	arg1	loss					339:342	weight loss	332:342	weight loss in humans	332:352	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	7	52	theme	receptor	1164:1171	arg1	type					1200:1203	HDL receptor scavenger receptor class B type 1	1160:1205	HDL receptor scavenger receptor class B type 1 in the aortic arch	1160:1224	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	10	53	from	effects	1857:1863	arg1	efflux					1880:1885	cholesterol efflux	1868:1885	cholesterol efflux	1868:1885	We conclude that 10,12 CLA may therefore exert its atheroprotective effects by increasing HDL-P concentration, HDL anti-inflammatory potential, and promoting beneficial effects on cholesterol efflux.					
35714730	4	54	theme	control	760:766	arg1	group					768:772	an obese control group	751:772	an obese control group consuming a high-fat high-sucrose diet	751:811	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	7	55	from	expression	1146:1155	arg1	arch					1221:1224	the aortic arch	1210:1224	the aortic arch	1210:1224	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	2	56	theme	linoleic	279:286	arg1	foods					255:259	ruminant animal foods	239:259	ruminant animal foods	239:259	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	2	56	theme	linoleic	279:286	arg1	acid					288:291	10,12-conjugated linoleic acid	262:291	10,12-conjugated linoleic acid (10,12 CLA)	262:303	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	2	56	theme	linoleic	279:286	arg1	CLA					300:302	10,12 CLA	294:302	10,12 CLA	294:302	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	9	57	theme	elevated	1458:1465	arg1	content					1473:1479	elevated Apoa1 content	1458:1479	elevated Apoa1 content in 10,12 CLA-HDL-Ps	1458:1499	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	6	58	theme	medium-	1040:1046	arg1	HDL-Ps					1064:1069	more medium- and large-sized HDL-Ps	1035:1069	more medium- and large-sized HDL-Ps	1035:1069	Furthermore, the 10,12 CLA-HDL particle (HDL-P) concentration was higher, attributed to more medium- and large-sized HDL-Ps.					
35714730	3	59	theme	weight	467:472	arg1	loss					474:477	its weight loss	463:477	its weight loss	463:477	Previous work has shown that 10,12 CLA is atheroprotective in mice by a mechanism that may be distinct from its weight loss effects, but this exact mechanism is unclear.					
35714730	9	60	theme	emerging	1613:1620	arg1	protein					1679:1685	an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein	1610:1685	an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein	1610:1685	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	9	60	theme	emerging	1613:1620	arg1	alpha-1-antitrypsin					1589:1607	alpha-1-antitrypsin	1589:1607	alpha-1-antitrypsin	1589:1607	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	0	61	from	function	76:83	arg1	mice					88:91	mice	88:91	mice	88:91	10,12-Conjugated linoleic acid supplementation improves HDL composition and function in mice.					
35714730	3	62	theme	exact	497:501	arg1	mechanism					503:511	this exact mechanism	492:511	this exact mechanism	492:511	Previous work has shown that 10,12 CLA is atheroprotective in mice by a mechanism that may be distinct from its weight loss effects, but this exact mechanism is unclear.					
35714730	4	63	theme	obese	591:595	arg1	mice					620:623	obese LDL receptor (Ldlr-/-) mice	591:623	obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group)	591:742	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	6	64	dep	higher	1013:1018	arg1	attributed					1021:1030	attributed	1021:1030	attributed to more medium- and large-sized HDL-Ps	1021:1069	Furthermore, the 10,12 CLA-HDL particle (HDL-P) concentration was higher, attributed to more medium- and large-sized HDL-Ps.					
35714730	1	65	theme	major	186:190	arg1	inflammation					121:132	inflammation	121:132	inflammation	121:132	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	1	65	theme	major	186:190	arg1	resistance					143:152	insulin resistance	135:152	insulin resistance	135:152	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	1	65	theme	major	186:190	arg1	factors					197:203	major risk factors	186:203	major risk factors for CVD	186:211	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	1	65	theme	major	186:190	arg1	diabetes					166:173	type 2 diabetes	159:173	type 2 diabetes	159:173	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	9	66	theme	HDL-associated	1664:1677	arg1	protein					1679:1685	an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein	1610:1685	an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein	1610:1685	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	9	66	theme	HDL-associated	1664:1677	arg1	alpha-1-antitrypsin					1589:1607	alpha-1-antitrypsin	1589:1607	alpha-1-antitrypsin	1589:1607	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	8	67	theme	increased	1365:1373	arg1	potential					1394:1402	increased cholesterol efflux potential	1365:1402	increased cholesterol efflux potential of these cells	1365:1417	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	2	68	theme	dietary	218:224	arg1	component					226:234	One dietary component	214:234	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA),	214:304	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	8	69	theme	efflux	1387:1392	arg1	potential					1394:1402	increased cholesterol efflux potential	1365:1402	increased cholesterol efflux potential of these cells	1365:1417	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	4	70	theme	high-fat	786:793	arg1	diet					808:811	a high-fat high-sucrose diet	784:811	a high-fat high-sucrose diet	784:811	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	2	71	theme	animal	248:253	arg1	foods					255:259	ruminant animal foods	239:259	ruminant animal foods	239:259	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	2	71	theme	animal	248:253	arg1	acid					288:291	10,12-conjugated linoleic acid	262:291	10,12-conjugated linoleic acid (10,12 CLA)	262:303	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	1	72	theme	insulin	135:141	arg1	inflammation					121:132	inflammation	121:132	inflammation	121:132	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	1	72	theme	insulin	135:141	arg1	resistance					143:152	insulin resistance	135:152	insulin resistance	135:152	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	1	72	theme	insulin	135:141	arg1	factors					197:203	major risk factors	186:203	major risk factors for CVD	186:211	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	1	72	theme	insulin	135:141	arg1	diabetes					166:173	type 2 diabetes	159:173	type 2 diabetes	159:173	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	8	73	theme	cells	1413:1417	arg1	potential					1394:1402	increased cholesterol efflux potential	1365:1402	increased cholesterol efflux potential of these cells	1365:1417	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	8	74	dep	transporters	1324:1335	arg1	transporters					1324:1335	cholesterol transporters Abca1 and Abcg1	1312:1351	cholesterol transporters Abca1 and Abcg1	1312:1351	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	8	74	dep	transporters	1324:1335	arg1	Abcg1					1347:1351	Abcg1	1347:1351	Abcg1	1347:1351	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	8	74	dep	transporters	1324:1335	arg1	Abca1					1337:1341	Abca1	1337:1341	Abca1	1337:1341	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	4	75	theme	receptor	601:608	arg1	mice					620:623	obese LDL receptor (Ldlr-/-) mice	591:623	obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group)	591:742	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	1	76	theme	type	159:162	arg1	diabetes					166:173	type 2 diabetes	159:173	type 2 diabetes	159:173	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	1	76	theme	type	159:162	arg1	resistance					143:152	insulin resistance	135:152	insulin resistance	135:152	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	1	76	theme	type	159:162	arg1	factors					197:203	major risk factors	186:203	major risk factors for CVD	186:211	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	1	76	theme	type	159:162	arg1	inflammation					121:132	inflammation	121:132	inflammation	121:132	Obesity is associated with inflammation, insulin resistance, and type 2 diabetes, which are major risk factors for CVD.					
35714730	7	77	theme	CLA-HDL	1117:1123	arg1	capacity					1099:1106	Passive cholesterol efflux capacity	1072:1106	Passive cholesterol efflux capacity of 10,12 CLA-HDL	1072:1123	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	5	78	theme	anti-inflammatory	860:876	arg1	effect					878:883	a stronger anti-inflammatory effect	849:883	a stronger anti-inflammatory effect	849:883	We show that 10,12 CLA-HDL exerted a stronger anti-inflammatory effect than CR- or high-fat high-sucrose-HDL in cultured adipocytes.					
35714730	4	79	theme	CLA	666:668	arg1	supplementation					670:684	10,12 CLA supplementation	660:684	10,12 CLA supplementation	660:684	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	9	80	theme	10,12	1484:1488	arg1	CLA-HDL-Ps					1490:1499	10,12 CLA-HDL-Ps	1484:1499	10,12 CLA-HDL-Ps	1484:1499	Finally, proteomics analysis revealed elevated Apoa1 content in 10,12 CLA-HDL-Ps, consistent with a higher particle concentration, and particles were also enriched with alpha-1-antitrypsin, an emerging anti-inflammatory and antiatherosclerotic HDL-associated protein.					
35714730	0	81	theme	linoleic	17:24	arg1	supplementation					31:45	10,12-Conjugated linoleic acid supplementation	0:45	10,12-Conjugated linoleic acid supplementation	0:45	10,12-Conjugated linoleic acid supplementation improves HDL composition and function in mice.					
35714730	7	82	dep	expression	1146:1155	arg1	as					1139:1140	as	1139:1140	as	1139:1140	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	7	82	dep	expression	1146:1155	arg1	was					1142:1144	was	1142:1144	was	1142:1144	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	7	83	theme	aortic	1214:1219	arg1	arch					1221:1224	the aortic arch	1210:1224	the aortic arch	1210:1224	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	8	84	theme	CLA	1265:1267	arg1	in vitro					1269:1276	10,12 CLA in vitro	1259:1276	10,12 CLA in vitro	1259:1276	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	3	85	theme	10,12	384:388	arg1	CLA					390:392	10,12 CLA	384:392	10,12 CLA	384:392	Previous work has shown that 10,12 CLA is atheroprotective in mice by a mechanism that may be distinct from its weight loss effects, but this exact mechanism is unclear.					
35714730	7	86	theme	Passive	1072:1078	arg1	efflux					1092:1097	Passive cholesterol efflux	1072:1097	Passive cholesterol efflux capacity of 10,12 CLA-HDL	1072:1123	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	10	87	theme	HDL	1799:1801	arg1	potential					1821:1829	HDL anti-inflammatory potential	1799:1829	HDL anti-inflammatory potential	1799:1829	We conclude that 10,12 CLA may therefore exert its atheroprotective effects by increasing HDL-P concentration, HDL anti-inflammatory potential, and promoting beneficial effects on cholesterol efflux.					
35714730	6	88	theme	CLA-HDL	970:976	arg1	HDL-P					988:992	HDL-P	988:992	HDL-P	988:992	Furthermore, the 10,12 CLA-HDL particle (HDL-P) concentration was higher, attributed to more medium- and large-sized HDL-Ps.					
35714730	6	88	theme	CLA-HDL	970:976	arg1	particle					978:985	10,12 CLA-HDL particle	964:985	the 10,12 CLA-HDL particle (HDL-P) concentration	960:1007	Furthermore, the 10,12 CLA-HDL particle (HDL-P) concentration was higher, attributed to more medium- and large-sized HDL-Ps.					
35714730	4	89	theme	caloric	689:695	arg1	CR					710:711	CR	710:711	CR; weight-matched control group	710:741	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	4	89	theme	caloric	689:695	arg1	restriction					697:707	caloric restriction	689:707	caloric restriction (CR; weight-matched control group)	689:742	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	10	90	theme	10,12	1705:1709	arg1	CLA					1711:1713	10,12 CLA	1705:1713	10,12 CLA	1705:1713	We conclude that 10,12 CLA may therefore exert its atheroprotective effects by increasing HDL-P concentration, HDL anti-inflammatory potential, and promoting beneficial effects on cholesterol efflux.					
35714730	0	91	theme	HDL	56:58	arg1	composition					60:70	HDL composition	56:70	HDL composition	56:70	10,12-Conjugated linoleic acid supplementation improves HDL composition and function in mice.					
35714730	5	92	theme	cultured	926:933	arg1	adipocytes					935:944	cultured adipocytes	926:944	cultured adipocytes	926:944	We show that 10,12 CLA-HDL exerted a stronger anti-inflammatory effect than CR- or high-fat high-sucrose-HDL in cultured adipocytes.					
35714730	7	93	theme	type	1200:1203	arg1	expression					1146:1155	expression	1146:1155	expression of HDL receptor scavenger receptor class B type 1 in the aortic arch	1146:1224	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	10	94	theme	beneficial	1846:1855	arg1	effects					1857:1863	promoting beneficial effects	1836:1863	promoting beneficial effects on cholesterol efflux	1836:1885	We conclude that 10,12 CLA may therefore exert its atheroprotective effects by increasing HDL-P concentration, HDL anti-inflammatory potential, and promoting beneficial effects on cholesterol efflux.					
35714730	4	95	theme	weight-matched	714:727	arg1	group					737:741	weight-matched control group	714:741	CR; weight-matched control group	710:741	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	3	96	from	atheroprotective	397:412	arg1	mice					417:420	mice	417:420	mice	417:420	Previous work has shown that 10,12 CLA is atheroprotective in mice by a mechanism that may be distinct from its weight loss effects, but this exact mechanism is unclear.					
35714730	2	97	from	loss	339:342	arg1	humans					347:352	humans	347:352	humans	347:352	One dietary component of ruminant animal foods, 10,12-conjugated linoleic acid (10,12 CLA), has been shown to promote weight loss in humans.					
35714730	7	98	theme	class	1192:1196	arg1	type					1200:1203	HDL receptor scavenger receptor class B type 1	1160:1205	HDL receptor scavenger receptor class B type 1 in the aortic arch	1160:1224	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	8	99	theme	cholesterol	1312:1322	arg1	transporters					1324:1335	cholesterol transporters Abca1 and Abcg1	1312:1351	cholesterol transporters Abca1 and Abcg1	1312:1351	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	8	99	theme	cholesterol	1312:1322	arg1	Abcg1					1347:1351	Abcg1	1347:1351	Abcg1	1347:1351	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	8	99	theme	cholesterol	1312:1322	arg1	Abca1					1337:1341	Abca1	1337:1341	Abca1	1337:1341	Murine macrophages treated with 10,12 CLA in vitro exhibited increased expression of cholesterol transporters Abca1 and Abcg1, suggesting increased cholesterol efflux potential of these cells.					
35714730	3	100	from	mice	417:420	arg1	atheroprotective					397:412	atheroprotective	397:412	atheroprotective	397:412	Previous work has shown that 10,12 CLA is atheroprotective in mice by a mechanism that may be distinct from its weight loss effects, but this exact mechanism is unclear.					
35714730	7	101	theme	scavenger	1173:1181	arg1	type					1200:1203	HDL receptor scavenger receptor class B type 1	1160:1205	HDL receptor scavenger receptor class B type 1 in the aortic arch	1160:1224	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	4	102	dep	receptor	601:608	arg1	Ldlr-/-					611:617	Ldlr-/-	611:617	Ldlr-/-	611:617	To investigate this, we evaluated HDL composition and function in obese LDL receptor (Ldlr-/-) mice that were losing weight because of 10,12 CLA supplementation or caloric restriction (CR; weight-matched control group) and in an obese control group consuming a high-fat high-sucrose diet.					
35714730	7	103	from	arch	1221:1224	arg1	expression					1146:1155	expression	1146:1155	expression of HDL receptor scavenger receptor class B type 1 in the aortic arch	1146:1224	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	7	104	theme	HDL	1160:1162	arg1	type					1200:1203	HDL receptor scavenger receptor class B type 1	1160:1205	HDL receptor scavenger receptor class B type 1 in the aortic arch	1160:1224	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35714730	7	105	theme	efflux	1092:1097	arg1	capacity					1099:1106	Passive cholesterol efflux capacity	1072:1106	Passive cholesterol efflux capacity of 10,12 CLA-HDL	1072:1123	Passive cholesterol efflux capacity of 10,12 CLA-HDL was elevated, as was expression of HDL receptor scavenger receptor class B type 1 in the aortic arch.					
35569536	14	0	theme	different	2644:2652	arg1	periods					2665:2671	different incubation periods	2644:2671	different incubation periods	2644:2671	No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety.					
35569536	4	1	theme	synthesised	935:945	arg1	nanocomposite					947:959	the synthesised nanocomposite	931:959	the synthesised nanocomposite	931:959	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	1	2	dep	in	421:422	arg1	vitro					424:428	vitro	424:428	vitro	424:428	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	13	3	dep	spectroscopy	2333:2344	arg1	studies					2392:2398	studies	2392:2398	studies	2392:2398	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies reveal that the nanocomposite was effectively coated on the packaging material without changing size, shape, and functional groups.					
35569536	7	4	theme	treated	1553:1559	arg1	strain					1568:1573	the nanocomposite treated fungal strain	1535:1573	the nanocomposite treated fungal strain	1535:1573	Among the various treatment, a notable reduction in all the tested marker genes expression was recorded in the nanocomposite treated fungal strain.					
35569536	2	5	theme	red	636:638	arg1	dye					640:642	congo red dye	630:642	congo red dye	630:642	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	7	6	theme	genes	1502:1506	arg1	expression					1508:1517	all the tested marker genes expression	1480:1517	all the tested marker genes expression	1480:1517	Among the various treatment, a notable reduction in all the tested marker genes expression was recorded in the nanocomposite treated fungal strain.					
35569536	14	7	theme	bread	2614:2618	arg1	samples					2620:2626	the packaged bread samples	2601:2626	the packaged bread samples incubated under different incubation periods	2601:2671	No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety.					
35569536	4	8	theme	antifungal	908:917	arg1	activity					919:926	the antifungal activity	904:926	the antifungal activity of the synthesised nanocomposite	904:959	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	7	9	theme	tested	1488:1493	arg1	genes					1502:1506	all the tested marker genes	1480:1506	all the tested marker genes expression	1480:1517	Among the various treatment, a notable reduction in all the tested marker genes expression was recorded in the nanocomposite treated fungal strain.					
35569536	12	10	theme	incubation	2274:2283	arg1	period					2285:2290	the incubation period	2270:2290	the incubation period	2270:2290	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	0	11	theme	Congo	205:209	arg1	efficacy					230:237	its marked Congo red dye adsorption efficacy	194:237	its marked Congo red dye adsorption efficacy	194:237	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	3	12	attach	isolated	744:751	arg2	strain					705:710	the fungal strain	694:710	the fungal strain Aspergillus ochraceopealiformis isolated from spoiled, expired bread	694:779	The antifungal activity was investigated against the fungal strain Aspergillus ochraceopealiformis isolated from spoiled, expired bread.					
35569536	3	12	attach	isolated	744:751	arg1	bread					775:779	spoiled, expired bread	758:779	spoiled, expired bread	758:779	The antifungal activity was investigated against the fungal strain Aspergillus ochraceopealiformis isolated from spoiled, expired bread.					
35569536	12	13	from	effect	2139:2144	arg1	status					2211:2216	antioxidative enzymes status	2189:2216	antioxidative enzymes status	2189:2216	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	12	13	from	effect	2139:2144	arg1	composition					2176:2186	the proximate composition	2162:2186	the proximate composition	2162:2186	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	12	13	from	effect	2139:2144	arg1	growth					2230:2235	fungal growth	2223:2235	fungal growth	2223:2235	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	6	14	theme	marker	1269:1274	arg1	genes					1276:1280	oxidative stress marker genes	1252:1280	oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR)	1252:1425	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	0	15	theme	spoilage	135:142	arg1	strain					151:156	food spoilage fungal strain Aspergillus ochraceopealiformis	130:188	food spoilage fungal strain Aspergillus ochraceopealiformis	130:188	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	0	16	theme	dye	215:217	arg1	efficacy					230:237	its marked Congo red dye adsorption efficacy	194:237	its marked Congo red dye adsorption efficacy	194:237	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	9	17	theme	animal	1799:1804	arg1	model					1806:1810	the Wistar animal model	1788:1810	the Wistar animal model	1788:1810	The synthesised nanocomposite's high safety or biocompatibility was evaluated with the Wistar animal model by determining notable changes in behavioural, biochemical, haematological and histopathological parameters.					
35569536	6	18	theme	oxidative	1252:1260	arg1	genes					1276:1280	oxidative stress marker genes	1252:1280	oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR)	1252:1425	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	12	19	theme	samples	2246:2252	arg1	status					2211:2216	antioxidative enzymes status	2189:2216	antioxidative enzymes status	2189:2216	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	12	19	theme	samples	2246:2252	arg1	growth					2230:2235	fungal growth	2223:2235	fungal growth	2223:2235	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	12	19	theme	samples	2246:2252	arg1	composition					2176:2186	the proximate composition	2162:2186	the proximate composition	2162:2186	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	16	20	theme	food	3022:3025	arg1	agent					3040:3044	an effective, safe food preservative agent	3003:3044	an effective, safe food preservative agent	3003:3044	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	16	20	theme	food	3022:3025	arg1	nanocomposite					2974:2986	the synthesised nanocomposite	2958:2986	the synthesised nanocomposite	2958:2986	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	5	21	theme	fungal	1165:1170	arg1	strain					1172:1177	the tested fungal strain	1154:1177	the tested fungal strain	1154:1177	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	2	22	theme	marked	571:576	arg1	activity					589:596	marked antifungal activity	571:596	marked antifungal activity	571:596	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	16	23	theme	chemicals	3069:3077	arg1	adsorbent					3050:3058	adsorbent	3050:3058	adsorbent	3050:3058	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	16	23	theme	chemicals	3069:3077	arg1	nanocomposite					2974:2986	the synthesised nanocomposite	2958:2986	the synthesised nanocomposite	2958:2986	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	16	24	theme	present	2929:2935	arg1	finding					2937:2943	The present finding	2925:2943	The present finding	2925:2943	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	1	25	theme	green	390:394	arg1	principles					404:413	green science principles	390:413	green science principles	390:413	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	11	26	theme	bread	2109:2113	arg1	material					2125:2132	the bread packaging material	2105:2132	the bread packaging material	2105:2132	The nanocomposite was coated on the bread packaging material.					
35569536	9	27	theme	notable	1827:1833	arg1	changes					1835:1841	notable changes	1827:1841	notable changes in behavioural, biochemical, haematological and histopathological parameters	1827:1918	The synthesised nanocomposite's high safety or biocompatibility was evaluated with the Wistar animal model by determining notable changes in behavioural, biochemical, haematological and histopathological parameters.					
35569536	3	28	dep	spoiled	758:764	arg1	expired					767:773	expired	767:773	expired	767:773	The antifungal activity was investigated against the fungal strain Aspergillus ochraceopealiformis isolated from spoiled, expired bread.					
35569536	0	29	theme	Aspergillus	158:168	arg1	strain					151:156	food spoilage fungal strain Aspergillus ochraceopealiformis	130:188	food spoilage fungal strain Aspergillus ochraceopealiformis	130:188	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	12	30	theme	fungal	2223:2228	arg1	growth					2230:2235	fungal growth	2223:2235	fungal growth	2223:2235	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	10	31	theme	marked	2035:2040	arg1	biosafety					2042:2050	the marked biosafety	2031:2050	the marked biosafety	2031:2050	The synthesised nanocomposite did not exhibit any undesirable changes in all the tested parameters confirming the marked biosafety or biocompatibility.					
35569536	6	32	theme	chain	1401:1405	arg1	reaction					1407:1414	quantitative real-time polymerase chain reaction	1367:1414	quantitative real-time polymerase chain reaction (q RT-PCR)	1367:1425	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	6	32	theme	chain	1401:1405	arg1	RT-PCR					1419:1424	q RT-PCR	1417:1424	q RT-PCR	1417:1424	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	5	33	theme	inhibition	1135:1144	arg1	zone					1052:1055	zone	1052:1055	zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition	1052:1144	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	5	34	theme	synthesised	999:1009	arg1	nanocomposite					1011:1023	the synthesised nanocomposite	995:1023	the synthesised nanocomposite	995:1023	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	7	35	from	reduction	1467:1475	arg1	expression					1508:1517	all the tested marker genes expression	1480:1517	all the tested marker genes expression	1480:1517	Among the various treatment, a notable reduction in all the tested marker genes expression was recorded in the nanocomposite treated fungal strain.					
35569536	2	36	theme	adsorption	602:611	arg1	efficacy					613:620	adsorption efficacy	602:620	adsorption efficacy	602:620	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	13	37	dep	transform	2314:2322	arg1	infrared					2324:2331	infrared	2324:2331	transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies	2314:2398	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies reveal that the nanocomposite was effectively coated on the packaging material without changing size, shape, and functional groups.					
35569536	6	38	theme	superoxide	1303:1312	arg1	catalase					1287:1294	catalase	1287:1294	catalase (CAT)	1287:1300	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	6	38	theme	superoxide	1303:1312	arg1	SOD					1325:1327	SOD	1325:1327	SOD	1325:1327	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	6	38	theme	superoxide	1303:1312	arg1	dismutase					1314:1322	superoxide dismutase	1303:1322	superoxide dismutase (SOD)	1303:1328	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	4	39	theme	well	786:789	arg1	assay					801:805	The well diffusion assay	782:805	The well diffusion assay	782:805	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	6	40	theme	real-time	1380:1388	arg1	reaction					1407:1414	quantitative real-time polymerase chain reaction	1367:1414	quantitative real-time polymerase chain reaction (q RT-PCR)	1367:1425	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	6	40	theme	real-time	1380:1388	arg1	RT-PCR					1419:1424	q RT-PCR	1417:1424	q RT-PCR	1417:1424	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	1	41	theme	in	421:422	arg1	conditions					430:439	in vitro conditions	421:439	in vitro conditions	421:439	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	16	42	dep	revealed	2839:2846	arg1	implies					2945:2951	implies	2945:2951	implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals	2945:3077	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	4	43	dep	assay	801:805	arg1	assay					875:879	inhibition assay	864:879	inhibition assay	864:879	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	12	44	theme	enzymes	2203:2209	arg1	status					2211:2216	antioxidative enzymes status	2189:2216	antioxidative enzymes status	2189:2216	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	0	45	theme	fungal	53:58	arg1	acacia-gold					71:81	anti fungal active gum acacia-gold	48:81	anti fungal active gum acacia-gold	48:81	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	1	46	theme	food	326:329	arg1	GA-AuNC-NT					361:370	GA-AuNC-NT	361:370	GA-AuNC-NT	361:370	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	1	46	theme	food	326:329	arg1	natamycin					350:358	food preservative agent natamycin	326:358	food preservative agent natamycin (GA-AuNC-NT)	326:371	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	3	47	theme	antifungal	649:658	arg1	activity					660:667	The antifungal activity	645:667	The antifungal activity	645:667	The antifungal activity was investigated against the fungal strain Aspergillus ochraceopealiformis isolated from spoiled, expired bread.					
35569536	5	48	theme	antifungal	972:981	arg1	activity					983:990	Potential antifungal activity	962:990	Potential antifungal activity of the synthesised nanocomposite	962:1023	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	9	49	from	changes	1835:1841	arg1	parameters					1909:1918	behavioural, biochemical, haematological and histopathological parameters	1846:1918	behavioural, biochemical, haematological and histopathological parameters	1846:1918	The synthesised nanocomposite's high safety or biocompatibility was evaluated with the Wistar animal model by determining notable changes in behavioural, biochemical, haematological and histopathological parameters.					
35569536	16	50	theme	toxic	3063:3067	arg1	chemicals					3069:3077	toxic chemicals	3063:3077	toxic chemicals	3063:3077	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	0	51	theme	gum	67:69	arg1	acacia-gold					71:81	anti fungal active gum acacia-gold	48:81	anti fungal active gum acacia-gold	48:81	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	1	52	theme	agent	344:348	arg1	GA-AuNC-NT					361:370	GA-AuNC-NT	361:370	GA-AuNC-NT	361:370	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	1	52	theme	agent	344:348	arg1	natamycin					350:358	food preservative agent natamycin	326:358	food preservative agent natamycin (GA-AuNC-NT)	326:371	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	4	53	theme	nanocomposite	947:959	arg1	activity					919:926	the antifungal activity	904:926	the antifungal activity of the synthesised nanocomposite	904:959	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	2	54	theme	nanocomposite	552:564	arg1	properties					522:531	highly stable structural, functional properties	485:531	highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye	485:642	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	8	55	theme	profile	1584:1590	arg1	studies					1592:1598	Release profile studies	1576:1598	Release profile studies using different solvents	1576:1623	Release profile studies using different solvents reveals sustained or controlled release of natamycin at the increasing periods.					
35569536	0	56	theme	food	130:133	arg1	spoilage					135:142	food spoilage	130:142	food spoilage fungal strain Aspergillus ochraceopealiformis	130:188	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	4	57	theme	fragmentation	822:834	arg1	assay					836:840	fungal hyphae fragmentation assay	808:840	fungal hyphae fragmentation assay	808:840	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	5	58	theme	inhibition	1060:1069	arg1	zone					1052:1055	zone	1052:1055	zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition	1052:1144	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	5	59	theme	high	1072:1075	arg1	rate					1077:1080	high rate	1072:1080	high rate of hyphae fragmentation	1072:1104	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	16	60	theme	Langmuir	2867:2874	arg1	mechanism					2876:2884	Langmuir mechanism	2867:2884	Langmuir mechanism	2867:2884	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	2	61	theme	characterisation	450:465	arg1	techniques					467:476	Various characterisation techniques	442:476	Various characterisation techniques	442:476	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	0	62	theme	Green	0:4	arg1	principles					16:25	Green chemistry principles	0:25	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold	0:81	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	2	63	with	nanocomposite	552:564	arg1	activity					589:596	marked antifungal activity	571:596	marked antifungal activity	571:596	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	2	63	with	nanocomposite	552:564	arg1	efficacy					613:620	adsorption efficacy	602:620	adsorption efficacy	602:620	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	8	64	theme	sustained	1633:1641	arg1	release					1657:1663	sustained or controlled release	1633:1663	sustained or controlled release of natamycin	1633:1676	Release profile studies using different solvents reveals sustained or controlled release of natamycin at the increasing periods.					
35569536	4	65	theme	fungal	808:813	arg1	assay					836:840	fungal hyphae fragmentation assay	808:840	fungal hyphae fragmentation assay	808:840	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	1	66	theme	gum	279:281	arg1	nanocomposite					296:308	a highly stable gum acacia -gold nanocomposite	263:308	a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT)	263:371	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	14	67	from	changes	2535:2541	arg1	composition					2560:2570	proximate composition	2550:2570	proximate composition	2550:2570	No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety.					
35569536	14	67	from	changes	2535:2541	arg1	enzymes					2590:2596	antioxidative enzymes	2576:2596	antioxidative enzymes	2576:2596	No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety.					
35569536	2	68	theme	stable	492:497	arg1	properties					522:531	highly stable structural, functional properties	485:531	highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye	485:642	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	8	69	theme	controlled	1646:1655	arg1	release					1657:1663	sustained or controlled release	1633:1663	sustained or controlled release of natamycin	1633:1676	Release profile studies using different solvents reveals sustained or controlled release of natamycin at the increasing periods.					
35569536	3	70	theme	fungal	698:703	arg1	strain					705:710	the fungal strain	694:710	the fungal strain Aspergillus ochraceopealiformis isolated from spoiled, expired bread	694:779	The antifungal activity was investigated against the fungal strain Aspergillus ochraceopealiformis isolated from spoiled, expired bread.					
35569536	1	71	theme	-gold	290:294	arg1	nanocomposite					296:308	a highly stable gum acacia -gold nanocomposite	263:308	a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT)	263:371	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	14	72	theme	marked	2700:2705	arg1	safety					2707:2712	the nanocomposite's marked safety	2680:2712	the nanocomposite's marked safety	2680:2712	No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety.					
35569536	5	73	theme	marked	1110:1115	arg1	germination					1123:1133	marked spore germination	1110:1133	marked spore germination inhibition	1110:1144	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	16	74	theme	model	2906:2910	arg1	utilisation					2852:2862	the utilisation	2848:2862	the utilisation of Langmuir mechanism and pseudo II order model	2848:2910	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	13	75	theme	scanning	2357:2364	arg1	SEM					2387:2389	SEM	2387:2389	SEM	2387:2389	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies reveal that the nanocomposite was effectively coated on the packaging material without changing size, shape, and functional groups.					
35569536	13	75	theme	scanning	2357:2364	arg1	microscopy					2375:2384	scanning electron microscopy	2357:2384	scanning electron microscopy (SEM)	2357:2390	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies reveal that the nanocomposite was effectively coated on the packaging material without changing size, shape, and functional groups.					
35569536	2	76	dep	structural	499:508	arg1	functional					511:520	functional	511:520	functional	511:520	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	5	77	theme	germination	1123:1133	arg1	inhibition					1135:1144	marked spore germination inhibition	1110:1144	marked spore germination inhibition	1110:1144	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	6	78	theme	activity	1218:1225	arg1	mechanism					1194:1202	The molecular mechanism	1180:1202	The molecular mechanism of antifungal activity	1180:1225	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	8	79	theme	increasing	1685:1694	arg1	periods					1696:1702	the increasing periods	1681:1702	the increasing periods	1681:1702	Release profile studies using different solvents reveals sustained or controlled release of natamycin at the increasing periods.					
35569536	10	80	theme	tested	2002:2007	arg1	parameters					2009:2018	all the tested parameters	1994:2018	all the tested parameters confirming the marked biosafety or biocompatibility	1994:2070	The synthesised nanocomposite did not exhibit any undesirable changes in all the tested parameters confirming the marked biosafety or biocompatibility.					
35569536	14	81	theme	proximate	2550:2558	arg1	composition					2560:2570	proximate composition	2550:2570	proximate composition	2550:2570	No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety.					
35569536	15	82	theme	fungal	2743:2748	arg1	growth					2750:2755	the fungal growth	2739:2755	the fungal growth	2739:2755	The complete absence of the fungal growth also indicates the uniqueness of the nanocomposite.					
35569536	10	83	from	changes	1983:1989	arg1	parameters					2009:2018	all the tested parameters	1994:2018	all the tested parameters confirming the marked biosafety or biocompatibility	1994:2070	The synthesised nanocomposite did not exhibit any undesirable changes in all the tested parameters confirming the marked biosafety or biocompatibility.					
35569536	7	84	theme	fungal	1561:1566	arg1	strain					1568:1573	the nanocomposite treated fungal strain	1535:1573	the nanocomposite treated fungal strain	1535:1573	Among the various treatment, a notable reduction in all the tested marker genes expression was recorded in the nanocomposite treated fungal strain.					
35569536	5	85	theme	fragmentation	1092:1104	arg1	inhibition					1060:1069	inhibition	1060:1069	inhibition	1060:1069	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	5	85	theme	fragmentation	1092:1104	arg1	inhibition					1135:1144	marked spore germination inhibition	1110:1144	marked spore germination inhibition	1110:1144	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	5	85	theme	fragmentation	1092:1104	arg1	rate					1077:1080	high rate	1072:1080	high rate of hyphae fragmentation	1072:1104	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	6	86	theme	quantitative	1367:1378	arg1	reaction					1407:1414	quantitative real-time polymerase chain reaction	1367:1414	quantitative real-time polymerase chain reaction (q RT-PCR)	1367:1425	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	6	86	theme	quantitative	1367:1378	arg1	RT-PCR					1419:1424	q RT-PCR	1417:1424	q RT-PCR	1417:1424	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	1	87	theme	present	248:254	arg1	study					256:260	this present study	243:260	this present study	243:260	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	7	88	theme	nanocomposite	1539:1551	arg1	strain					1568:1573	the nanocomposite treated fungal strain	1535:1573	the nanocomposite treated fungal strain	1535:1573	Among the various treatment, a notable reduction in all the tested marker genes expression was recorded in the nanocomposite treated fungal strain.					
35569536	6	89	theme	molecular	1184:1192	arg1	mechanism					1194:1202	The molecular mechanism	1180:1202	The molecular mechanism of antifungal activity	1180:1225	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	3	90	theme	spoiled	758:764	arg1	bread					775:779	spoiled, expired bread	758:779	spoiled, expired bread	758:779	The antifungal activity was investigated against the fungal strain Aspergillus ochraceopealiformis isolated from spoiled, expired bread.					
35569536	0	91	theme	marked	198:203	arg1	efficacy					230:237	its marked Congo red dye adsorption efficacy	194:237	its marked Congo red dye adsorption efficacy	194:237	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	15	92	theme	complete	2719:2726	arg1	absence					2728:2734	The complete absence	2715:2734	The complete absence of the fungal growth	2715:2755	The complete absence of the fungal growth also indicates the uniqueness of the nanocomposite.					
35569536	16	93	theme	effective	3006:3014	arg1	agent					3040:3044	an effective, safe food preservative agent	3003:3044	an effective, safe food preservative agent	3003:3044	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	16	93	theme	effective	3006:3014	arg1	nanocomposite					2974:2986	the synthesised nanocomposite	2958:2986	the synthesised nanocomposite	2958:2986	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	14	94	theme	samples	2620:2626	arg1	composition					2560:2570	proximate composition	2550:2570	proximate composition	2550:2570	No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety.					
35569536	14	94	theme	samples	2620:2626	arg1	enzymes					2590:2596	antioxidative enzymes	2576:2596	antioxidative enzymes	2576:2596	No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety.					
35569536	2	95	theme	congo	630:634	arg1	dye					640:642	congo red dye	630:642	congo red dye	630:642	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	6	96	theme	catalase	1287:1294	arg1	induction					1348:1356	catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction	1287:1356	catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR)	1287:1425	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	15	97	theme	nanocomposite	2794:2806	arg1	uniqueness					2776:2785	the uniqueness	2772:2785	the uniqueness of the nanocomposite	2772:2806	The complete absence of the fungal growth also indicates the uniqueness of the nanocomposite.					
35569536	0	98	theme	red	211:213	arg1	efficacy					230:237	its marked Congo red dye adsorption efficacy	194:237	its marked Congo red dye adsorption efficacy	194:237	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	12	99	theme	packaging	2149:2157	arg1	effect					2139:2144	The effect	2135:2144	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period	2135:2290	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	14	100	theme	packaged	2605:2612	arg1	samples					2620:2626	the packaged bread samples	2601:2626	the packaged bread samples incubated under different incubation periods	2601:2671	No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety.					
35569536	7	101	theme	marker	1495:1500	arg1	genes					1502:1506	all the tested marker genes	1480:1506	all the tested marker genes expression	1480:1517	Among the various treatment, a notable reduction in all the tested marker genes expression was recorded in the nanocomposite treated fungal strain.					
35569536	9	102	theme	haematological	1872:1885	arg1	parameters					1909:1918	behavioural, biochemical, haematological and histopathological parameters	1846:1918	behavioural, biochemical, haematological and histopathological parameters	1846:1918	The synthesised nanocomposite's high safety or biocompatibility was evaluated with the Wistar animal model by determining notable changes in behavioural, biochemical, haematological and histopathological parameters.					
35569536	0	103	theme	adsorption	219:228	arg1	efficacy					230:237	its marked Congo red dye adsorption efficacy	194:237	its marked Congo red dye adsorption efficacy	194:237	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	4	104	used	used	886:889	arg2	assay					836:840	fungal hyphae fragmentation assay	808:840	fungal hyphae fragmentation assay	808:840	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	4	104	used	used	886:889	arg2	germination					852:862	spore germination	846:862	spore germination	846:862	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	4	104	used	used	886:889	arg2	assay					801:805	The well diffusion assay	782:805	The well diffusion assay	782:805	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	9	105	theme	Wistar	1792:1797	arg1	model					1806:1810	the Wistar animal model	1788:1810	the Wistar animal model	1788:1810	The synthesised nanocomposite's high safety or biocompatibility was evaluated with the Wistar animal model by determining notable changes in behavioural, biochemical, haematological and histopathological parameters.					
35569536	6	106	theme	stress	1262:1267	arg1	genes					1276:1280	oxidative stress marker genes	1252:1280	oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR)	1252:1425	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	16	107	theme	preservative	3027:3038	arg1	agent					3040:3044	an effective, safe food preservative agent	3003:3044	an effective, safe food preservative agent	3003:3044	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	16	107	theme	preservative	3027:3038	arg1	nanocomposite					2974:2986	the synthesised nanocomposite	2958:2986	the synthesised nanocomposite	2958:2986	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	2	108	theme	antifungal	578:587	arg1	activity					589:596	marked antifungal activity	571:596	marked antifungal activity	571:596	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	12	109	theme	bread	2240:2244	arg1	samples					2246:2252	bread samples	2240:2252	bread samples incubated under the incubation period	2240:2290	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	6	110	theme	q	1417:1417	arg1	reaction					1407:1414	quantitative real-time polymerase chain reaction	1367:1414	quantitative real-time polymerase chain reaction (q RT-PCR)	1367:1425	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	6	110	theme	q	1417:1417	arg1	RT-PCR					1419:1424	q RT-PCR	1417:1424	q RT-PCR	1417:1424	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	13	111	theme	packaging	2460:2468	arg1	material					2470:2477	the packaging material	2456:2477	the packaging material	2456:2477	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies reveal that the nanocomposite was effectively coated on the packaging material without changing size, shape, and functional groups.					
35569536	7	112	theme	notable	1459:1465	arg1	reduction					1467:1475	a notable reduction	1457:1475	a notable reduction in all the tested marker genes expression	1457:1517	Among the various treatment, a notable reduction in all the tested marker genes expression was recorded in the nanocomposite treated fungal strain.					
35569536	13	113	theme	electron	2366:2373	arg1	SEM					2387:2389	SEM	2387:2389	SEM	2387:2389	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies reveal that the nanocomposite was effectively coated on the packaging material without changing size, shape, and functional groups.					
35569536	13	113	theme	electron	2366:2373	arg1	microscopy					2375:2384	scanning electron microscopy	2357:2384	scanning electron microscopy (SEM)	2357:2390	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies reveal that the nanocomposite was effectively coated on the packaging material without changing size, shape, and functional groups.					
35569536	0	114	theme	fungal	144:149	arg1	strain					151:156	food spoilage fungal strain Aspergillus ochraceopealiformis	130:188	food spoilage fungal strain Aspergillus ochraceopealiformis	130:188	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	1	115	theme	science	396:402	arg1	principles					404:413	green science principles	390:413	green science principles	390:413	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	10	116	theme	synthesised	1925:1935	arg1	nanocomposite					1937:1949	The synthesised nanocomposite	1921:1949	The synthesised nanocomposite	1921:1949	The synthesised nanocomposite did not exhibit any undesirable changes in all the tested parameters confirming the marked biosafety or biocompatibility.					
35569536	0	117	dep	Aspergillus	158:168	arg1	ochraceopealiformis					170:188	ochraceopealiformis	170:188	ochraceopealiformis	170:188	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	13	118	dep	Fourier	2306:2312	arg1	transform					2314:2322	transform	2314:2322	transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies	2314:2398	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies reveal that the nanocomposite was effectively coated on the packaging material without changing size, shape, and functional groups.					
35569536	14	119	dep	composition	2560:2570	arg1	the					2546:2548	the	2546:2548	the	2546:2548	No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety.					
35569536	16	120	used	used	2995:2998	arg2	nanocomposite					2974:2986	the synthesised nanocomposite	2958:2986	the synthesised nanocomposite	2958:2986	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	16	120	used	used	2995:2998	arg2	adsorbent					3050:3058	adsorbent	3050:3058	adsorbent	3050:3058	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	16	120	used	used	2995:2998	arg2	agent					3040:3044	an effective, safe food preservative agent	3003:3044	an effective, safe food preservative agent	3003:3044	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	6	121	theme	polymerase	1390:1399	arg1	reaction					1407:1414	quantitative real-time polymerase chain reaction	1367:1414	quantitative real-time polymerase chain reaction (q RT-PCR)	1367:1425	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	6	121	theme	polymerase	1390:1399	arg1	RT-PCR					1419:1424	q RT-PCR	1417:1424	q RT-PCR	1417:1424	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	9	122	theme	synthesised	1709:1719	arg1	safety					1742:1747	nanocomposite's high safety	1721:1747	nanocomposite's high safety	1721:1747	The synthesised nanocomposite's high safety or biocompatibility was evaluated with the Wistar animal model by determining notable changes in behavioural, biochemical, haematological and histopathological parameters.					
35569536	10	123	theme	undesirable	1971:1981	arg1	changes					1983:1989	any undesirable changes	1967:1989	any undesirable changes in all the tested parameters confirming the marked biosafety or biocompatibility	1967:2070	The synthesised nanocomposite did not exhibit any undesirable changes in all the tested parameters confirming the marked biosafety or biocompatibility.					
35569536	5	124	theme	nanocomposite	1011:1023	arg1	activity					983:990	Potential antifungal activity	962:990	Potential antifungal activity of the synthesised nanocomposite	962:1023	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	9	125	theme	behavioural	1846:1856	arg1	parameters					1909:1918	behavioural, biochemical, haematological and histopathological parameters	1846:1918	behavioural, biochemical, haematological and histopathological parameters	1846:1918	The synthesised nanocomposite's high safety or biocompatibility was evaluated with the Wistar animal model by determining notable changes in behavioural, biochemical, haematological and histopathological parameters.					
35569536	7	126	theme	various	1438:1444	arg1	treatment					1446:1454	the various treatment	1434:1454	the various treatment	1434:1454	Among the various treatment, a notable reduction in all the tested marker genes expression was recorded in the nanocomposite treated fungal strain.					
35569536	0	127	theme	active	60:65	arg1	acacia-gold					71:81	anti fungal active gum acacia-gold	48:81	anti fungal active gum acacia-gold	48:81	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	5	128	theme	tested	1158:1163	arg1	strain					1172:1177	the tested fungal strain	1154:1177	the tested fungal strain	1154:1177	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	11	129	theme	packaging	2115:2123	arg1	material					2125:2132	the bread packaging material	2105:2132	the bread packaging material	2105:2132	The nanocomposite was coated on the bread packaging material.					
35569536	5	130	theme	Potential	962:970	arg1	activity					983:990	Potential antifungal activity	962:990	Potential antifungal activity of the synthesised nanocomposite	962:1023	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	9	131	theme	biochemical	1859:1869	arg1	parameters					1909:1918	behavioural, biochemical, haematological and histopathological parameters	1846:1918	behavioural, biochemical, haematological and histopathological parameters	1846:1918	The synthesised nanocomposite's high safety or biocompatibility was evaluated with the Wistar animal model by determining notable changes in behavioural, biochemical, haematological and histopathological parameters.					
35569536	8	132	theme	Release	1576:1582	arg1	studies					1592:1598	Release profile studies	1576:1598	Release profile studies using different solvents	1576:1623	Release profile studies using different solvents reveals sustained or controlled release of natamycin at the increasing periods.					
35569536	16	133	theme	synthesised	2962:2972	arg1	agent					3040:3044	an effective, safe food preservative agent	3003:3044	an effective, safe food preservative agent	3003:3044	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	16	133	theme	synthesised	2962:2972	arg1	adsorbent					3050:3058	adsorbent	3050:3058	adsorbent	3050:3058	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	16	133	theme	synthesised	2962:2972	arg1	nanocomposite					2974:2986	the synthesised nanocomposite	2958:2986	the synthesised nanocomposite	2958:2986	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	12	134	theme	antioxidative	2189:2201	arg1	status					2211:2216	antioxidative enzymes status	2189:2216	antioxidative enzymes status	2189:2216	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	9	135	theme	histopathological	1891:1907	arg1	parameters					1909:1918	behavioural, biochemical, haematological and histopathological parameters	1846:1918	behavioural, biochemical, haematological and histopathological parameters	1846:1918	The synthesised nanocomposite's high safety or biocompatibility was evaluated with the Wistar animal model by determining notable changes in behavioural, biochemical, haematological and histopathological parameters.					
35569536	0	136	theme	acacia-gold	71:81	arg1	synthesis					35:43	the synthesis	31:43	the synthesis of anti fungal active gum acacia-gold	31:81	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	1	137	theme	preservative	331:342	arg1	GA-AuNC-NT					361:370	GA-AuNC-NT	361:370	GA-AuNC-NT	361:370	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	1	137	theme	preservative	331:342	arg1	natamycin					350:358	food preservative agent natamycin	326:358	food preservative agent natamycin (GA-AuNC-NT)	326:371	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	2	138	theme	structural	499:508	arg1	properties					522:531	highly stable structural, functional properties	485:531	highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye	485:642	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	2	139	theme	synthesised	540:550	arg1	nanocomposite					552:564	the synthesised nanocomposite	536:564	the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye	536:642	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	8	140	theme	different	1606:1614	arg1	solvents					1616:1623	different solvents	1606:1623	different solvents	1606:1623	Release profile studies using different solvents reveals sustained or controlled release of natamycin at the increasing periods.					
35569536	4	141	theme	hyphae	815:820	arg1	assay					836:840	fungal hyphae fragmentation assay	808:840	fungal hyphae fragmentation assay	808:840	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	16	142	theme	sorption	2822:2829	arg1	studies					2831:2837	the sorption studies	2818:2837	the sorption studies	2818:2837	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	16	143	dep	effective	3006:3014	arg1	safe					3017:3020	safe	3017:3020	safe	3017:3020	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	16	144	theme	mechanism	2876:2884	arg1	utilisation					2852:2862	the utilisation	2848:2862	the utilisation of Langmuir mechanism and pseudo II order model	2848:2910	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	0	145	theme	chemistry	6:14	arg1	principles					16:25	Green chemistry principles	0:25	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold	0:81	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	16	146	theme	pseudo	2890:2895	arg1	model					2906:2910	pseudo II order model	2890:2910	pseudo II order model	2890:2910	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	2	147	theme	Various	442:448	arg1	techniques					467:476	Various characterisation techniques	442:476	Various characterisation techniques	442:476	Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye.					
35569536	4	148	theme	diffusion	791:799	arg1	assay					801:805	The well diffusion assay	782:805	The well diffusion assay	782:805	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	1	149	theme	stable	272:277	arg1	nanocomposite					296:308	a highly stable gum acacia -gold nanocomposite	263:308	a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT)	263:371	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	16	150	theme	order	2900:2904	arg1	model					2906:2910	pseudo II order model	2890:2910	pseudo II order model	2890:2910	Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.					
35569536	1	151	theme	acacia	283:288	arg1	nanocomposite					296:308	a highly stable gum acacia -gold nanocomposite	263:308	a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT)	263:371	In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions.					
35569536	8	152	theme	natamycin	1668:1676	arg1	release					1657:1663	sustained or controlled release	1633:1663	sustained or controlled release of natamycin	1633:1676	Release profile studies using different solvents reveals sustained or controlled release of natamycin at the increasing periods.					
35569536	0	153	theme	anti	48:51	arg1	acacia-gold					71:81	anti fungal active gum acacia-gold	48:81	anti fungal active gum acacia-gold	48:81	Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.					
35569536	13	154	theme	functional	2513:2522	arg1	groups					2524:2529	functional groups	2513:2529	functional groups	2513:2529	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies reveal that the nanocomposite was effectively coated on the packaging material without changing size, shape, and functional groups.					
35569536	5	155	theme	spore	1117:1121	arg1	germination					1123:1133	marked spore germination	1110:1133	marked spore germination inhibition	1110:1144	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	14	156	theme	incubation	2654:2663	arg1	periods					2665:2671	different incubation periods	2644:2671	different incubation periods	2644:2671	No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety.					
35569536	14	157	theme	antioxidative	2576:2588	arg1	enzymes					2590:2596	antioxidative enzymes	2576:2596	antioxidative enzymes	2576:2596	No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety.					
35569536	15	158	theme	growth	2750:2755	arg1	absence					2728:2734	The complete absence	2715:2734	The complete absence of the fungal growth	2715:2755	The complete absence of the fungal growth also indicates the uniqueness of the nanocomposite.					
35569536	9	159	theme	high	1737:1740	arg1	safety					1742:1747	nanocomposite's high safety	1721:1747	nanocomposite's high safety	1721:1747	The synthesised nanocomposite's high safety or biocompatibility was evaluated with the Wistar animal model by determining notable changes in behavioural, biochemical, haematological and histopathological parameters.					
35569536	5	160	theme	rate	1077:1080	arg1	zone					1052:1055	zone	1052:1055	zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition	1052:1144	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	4	161	theme	spore	846:850	arg1	germination					852:862	spore germination	846:862	spore germination	846:862	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
35569536	12	162	theme	proximate	2166:2174	arg1	composition					2176:2186	the proximate composition	2162:2186	the proximate composition	2162:2186	The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied.					
35569536	5	163	theme	hyphae	1085:1090	arg1	fragmentation					1092:1104	hyphae fragmentation	1085:1104	hyphae fragmentation	1085:1104	Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain.					
35569536	6	164	theme	antifungal	1207:1216	arg1	activity					1218:1225	antifungal activity	1207:1225	antifungal activity	1207:1225	The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR).					
35569536	4	165	theme	inhibition	864:873	arg1	assay					875:879	inhibition assay	864:879	inhibition assay	864:879	The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite.					
36017665	0	0	theme	LPS-stimulated	102:115	arg1	macrophages					124:134	LPS-stimulated murine macrophages	102:134	LPS-stimulated murine macrophages	102:134	Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages.					
36017665	8	1	dep	CONCLUSIONS	1160:1170	arg1	suggest					1185:1191	suggest	1185:1191	suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders	1185:1382	CONCLUSIONS Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.					
36017665	5	2	theme	oxygen	714:719	arg1	species					721:727	Reactive oxygen species	705:727	Reactive oxygen species (ROS) generation	705:744	Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry.					
36017665	5	2	theme	oxygen	714:719	arg1	ROS					730:732	ROS	730:732	ROS	730:732	Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry.					
36017665	8	3	theme	macrophage	1221:1230	arg1	activity					1245:1252	macrophage inflammatory activity	1221:1252	macrophage inflammatory activity	1221:1252	CONCLUSIONS Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.					
36017665	1	4	theme	choleretic	199:208	arg1	properties					210:219	hepatoprotective and choleretic properties	178:219	hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants	178:281	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36017665	5	5	theme	ROS-sensitive	765:777	arg1	H2DCFDA					803:809	H2DCFDA	803:809	H2DCFDA	803:809	Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry.					
36017665	5	5	theme	ROS-sensitive	765:777	arg1	indicator					792:800	ROS-sensitive fluorescence indicator	765:800	ROS-sensitive fluorescence indicator	765:800	Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry.					
36017665	0	6	from	effect	18:23	arg1	macrophages					124:134	LPS-stimulated murine macrophages	102:134	LPS-stimulated murine macrophages	102:134	Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages.					
36017665	5	7	theme	species	721:727	arg1	generation					735:744	Reactive oxygen species (ROS) generation	705:744	Reactive oxygen species (ROS) generation	705:744	Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry.					
36017665	7	8	theme	LPS-stimulated	967:980	arg1	macrophages					982:992	LPS-stimulated macrophages	967:992	LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation	967:1057	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	8	9	theme	typical	1349:1355	arg1	inflammation					1336:1347	chronic inflammation	1328:1347	chronic inflammation typical of hepatobiliary disorders	1328:1382	CONCLUSIONS Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.					
36017665	8	10	theme	important	1271:1279	arg1	rationale					1281:1289	an important rationale	1268:1289	an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders	1268:1382	CONCLUSIONS Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.					
36017665	7	11	dep	RESULTS	886:892	arg1	exerted					929:935	exerted	929:935	exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release	929:1157	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	7	12	theme	arginine	1075:1082	arg1	metabolism					1084:1093	arginine metabolism	1075:1093	arginine metabolism	1075:1093	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	0	13	theme	murine	117:122	arg1	macrophages					124:134	LPS-stimulated murine macrophages	102:134	LPS-stimulated murine macrophages	102:134	Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages.					
36017665	3	14	theme	color	573:577	arg1	reactions					579:587	specific color reactions	564:587	specific color reactions	564:587	METHODS The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC-MS).					
36017665	1	15	with	formulation	161:171	arg1	properties					210:219	hepatoprotective and choleretic properties	178:219	hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants	178:281	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36017665	4	16	theme	Griess	688:693	arg1	reaction					695:702	the Griess reaction	684:702	the Griess reaction	684:702	Nitric oxide (NO) assay was determined using the Griess reaction.					
36017665	3	17	theme	chromatography-mass	597:615	arg1	GC-MS					631:635	GC-MS	631:635	GC-MS	631:635	METHODS The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC-MS).					
36017665	3	17	theme	chromatography-mass	597:615	arg1	spectrometry					617:628	gas chromatography-mass spectrometry	593:628	gas chromatography-mass spectrometry (GC-MS)	593:636	METHODS The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC-MS).					
36017665	8	18	theme	chronic	1328:1334	arg1	inflammation					1336:1347	chronic inflammation	1328:1347	chronic inflammation typical of hepatobiliary disorders	1328:1382	CONCLUSIONS Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.					
36017665	7	19	theme	activity	1123:1130	arg1	decrease					1136:1143	decrease	1136:1143	decrease of NO release	1136:1157	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	7	19	theme	activity	1123:1130	arg1	increase					1102:1109	the increase	1098:1109	the increase of arginase activity	1098:1130	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	7	20	theme	generation	1048:1057	arg1	decrease					1032:1039	dose-dependent decrease	1017:1039	dose-dependent decrease of ROS generation	1017:1057	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	1	21	theme	pharmacological	231:245	arg1	potential					247:255	pharmacological potential	231:255	pharmacological potential of eight medicinal plants	231:281	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36017665	3	22	theme	gas	593:595	arg1	GC-MS					631:635	GC-MS	631:635	GC-MS	631:635	METHODS The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC-MS).					
36017665	3	22	theme	gas	593:595	arg1	spectrometry					617:628	gas chromatography-mass spectrometry	593:628	gas chromatography-mass spectrometry (GC-MS)	593:636	METHODS The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC-MS).					
36017665	2	23	from	effect	389:394	arg1	metabolism					429:438	the metabolism	425:438	the metabolism of LPS-stimulated macrophages in vitro	425:477	To explore the effect of polyherbal composition on the metabolism of LPS-stimulated macrophages in vitro.					
36017665	7	24	theme	anti-inflammatory	937:953	arg1	activity					955:962	anti-inflammatory activity	937:962	anti-inflammatory activity	937:962	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	8	25	theme	disorders	1374:1382	arg1	typical					1349:1355	typical	1349:1355	typical	1349:1355	CONCLUSIONS Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.					
36017665	0	26	theme	Anti-inflammatory	0:16	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages.	0:135	Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages.					
36017665	1	27	theme	Azerbaijan	335:344	arg1	Republic					323:330	Republic	323:330	Republic of Azerbaijan	323:344	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36017665	5	28	theme	Reactive	705:712	arg1	species					721:727	Reactive oxygen species	705:727	Reactive oxygen species (ROS) generation	705:744	Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry.					
36017665	5	28	theme	Reactive	705:712	arg1	ROS					730:732	ROS	730:732	ROS	730:732	Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry.					
36017665	7	29	theme	polyherbal	906:915	arg1	formulation					917:927	The studied polyherbal formulation	894:927	The studied polyherbal formulation	894:927	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	8	30	theme	hepatobiliary	1360:1372	arg1	disorders					1374:1382	hepatobiliary disorders	1360:1382	hepatobiliary disorders	1360:1382	CONCLUSIONS Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.					
36017665	0	31	theme	polyherbal	28:37	arg1	composition					39:49	polyherbal composition	28:49	polyherbal composition	28:49	Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages.					
36017665	6	32	theme	colorimetric	865:876	arg1	method					878:883	colorimetric method	865:883	colorimetric method	865:883	Arginase activity was examined by colorimetric method.					
36017665	3	33	theme	phytochemical	521:533	arg1	analysis					535:542	The qualitative and quantitative phytochemical analysis	488:542	METHODS The qualitative and quantitative phytochemical analysis	480:542	METHODS The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC-MS).					
36017665	4	34	theme	oxide	646:650	arg1	assay					657:661	Nitric oxide (NO) assay	639:661	Nitric oxide (NO) assay	639:661	Nitric oxide (NO) assay was determined using the Griess reaction.					
36017665	2	35	theme	composition	410:420	arg1	effect					389:394	the effect	385:394	the effect of polyherbal composition on the metabolism of LPS-stimulated macrophages in vitro	385:477	To explore the effect of polyherbal composition on the metabolism of LPS-stimulated macrophages in vitro.					
36017665	1	36	theme	medicinal	266:274	arg1	plants					276:281	eight medicinal plants	260:281	eight medicinal plants	260:281	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36017665	3	37	theme	specific	564:571	arg1	reactions					579:587	specific color reactions	564:587	specific color reactions	564:587	METHODS The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC-MS).					
36017665	5	38	theme	fluorescence	779:790	arg1	H2DCFDA					803:809	H2DCFDA	803:809	H2DCFDA	803:809	Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry.					
36017665	5	38	theme	fluorescence	779:790	arg1	indicator					792:800	ROS-sensitive fluorescence indicator	765:800	ROS-sensitive fluorescence indicator	765:800	Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry.					
36017665	2	39	theme	macrophages	458:468	arg1	metabolism					429:438	the metabolism	425:438	the metabolism of LPS-stimulated macrophages in vitro	425:477	To explore the effect of polyherbal composition on the metabolism of LPS-stimulated macrophages in vitro.					
36017665	7	40	theme	studied	898:904	arg1	formulation					917:927	The studied polyherbal formulation	894:927	The studied polyherbal formulation	894:927	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	1	41	theme	plants	276:281	arg1	potential					247:255	pharmacological potential	231:255	pharmacological potential of eight medicinal plants	231:281	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36017665	0	42	theme	composition	39:49	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages.	0:135	Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages.					
36017665	3	43	dep	METHODS	480:486	arg1	analysis					535:542	The qualitative and quantitative phytochemical analysis	488:542	METHODS The qualitative and quantitative phytochemical analysis	480:542	METHODS The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC-MS).					
36017665	4	44	theme	Nitric	639:644	arg1	NO					653:654	NO	653:654	NO	653:654	Nitric oxide (NO) assay was determined using the Griess reaction.					
36017665	4	44	theme	Nitric	639:644	arg1	oxide					646:650	Nitric oxide	639:650	Nitric oxide (NO) assay	639:661	Nitric oxide (NO) assay was determined using the Griess reaction.					
36017665	2	45	theme	LPS-stimulated	443:456	arg1	macrophages					458:468	LPS-stimulated macrophages	443:468	LPS-stimulated macrophages	443:468	To explore the effect of polyherbal composition on the metabolism of LPS-stimulated macrophages in vitro.					
36017665	8	46	theme	inflammation	1336:1347	arg1	attenuation					1313:1323	the attenuation	1309:1323	the attenuation of chronic inflammation typical of hepatobiliary disorders	1309:1382	CONCLUSIONS Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.					
36017665	7	47	theme	NO	1148:1149	arg1	release					1151:1157	NO release	1148:1157	NO release	1148:1157	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	8	48	theme	inflammatory	1232:1243	arg1	activity					1245:1252	macrophage inflammatory activity	1221:1252	macrophage inflammatory activity	1221:1252	CONCLUSIONS Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.					
36017665	0	49	theme	hepatoprotective	56:71	arg1	properties					88:97	hepatoprotective and choleretic properties	56:97	hepatoprotective and choleretic properties	56:97	Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages.					
36017665	1	50	theme	herbal	362:367	arg1	tea					369:371	herbal tea	362:371	herbal tea	362:371	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36017665	7	51	theme	release	1151:1157	arg1	decrease					1136:1143	decrease	1136:1143	decrease of NO release	1136:1157	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	7	51	theme	release	1151:1157	arg1	increase					1102:1109	the increase	1098:1109	the increase of arginase activity	1098:1130	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	0	52	with	effect	18:23	arg1	properties					88:97	hepatoprotective and choleretic properties	56:97	hepatoprotective and choleretic properties	56:97	Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages.					
36017665	1	53	theme	polyherbal	150:159	arg1	formulation					161:171	A polyherbal formulation	148:171	A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants	148:281	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36017665	7	54	theme	dose-dependent	1017:1030	arg1	decrease					1032:1039	dose-dependent decrease	1017:1039	dose-dependent decrease of ROS generation	1017:1057	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	7	55	theme	ROS	1044:1046	arg1	generation					1048:1057	ROS generation	1044:1057	ROS generation	1044:1057	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	8	56	theme	herbal	1202:1207	arg1	tea					1209:1211	the herbal tea	1198:1211	the herbal tea	1198:1211	CONCLUSIONS Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.					
36017665	2	57	from	metabolism	429:438	arg1	vitro					473:477	vitro	473:477	vitro	473:477	To explore the effect of polyherbal composition on the metabolism of LPS-stimulated macrophages in vitro.					
36017665	7	58	theme	arginase	1114:1121	arg1	activity					1123:1130	arginase activity	1114:1130	arginase activity	1114:1130	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	1	59	dep	center	315:320	arg1	Republic					323:330	Republic	323:330	Republic of Azerbaijan	323:344	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36017665	6	60	theme	Arginase	831:838	arg1	activity					840:847	Arginase activity	831:847	Arginase activity	831:847	Arginase activity was examined by colorimetric method.					
36017665	2	61	theme	polyherbal	399:408	arg1	composition					410:420	polyherbal composition	399:420	polyherbal composition	399:420	To explore the effect of polyherbal composition on the metabolism of LPS-stimulated macrophages in vitro.					
36017665	5	62	theme	flow	815:818	arg1	cytometry					820:828	flow cytometry	815:828	flow cytometry	815:828	Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry.					
36017665	3	63	theme	quantitative	508:519	arg1	analysis					535:542	The qualitative and quantitative phytochemical analysis	488:542	METHODS The qualitative and quantitative phytochemical analysis	480:542	METHODS The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC-MS).					
36017665	1	64	theme	hepatoprotective	178:193	arg1	properties					210:219	hepatoprotective and choleretic properties	178:219	hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants	178:281	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36017665	3	65	theme	qualitative	492:502	arg1	analysis					535:542	The qualitative and quantitative phytochemical analysis	488:542	METHODS The qualitative and quantitative phytochemical analysis	480:542	METHODS The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC-MS).					
36017665	1	66	theme	Nargiz	300:305	arg1	center					315:320	Nargiz Medical center	300:320	Nargiz Medical center (Republic of Azerbaijan)	300:345	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36017665	0	67	theme	choleretic	77:86	arg1	properties					88:97	hepatoprotective and choleretic properties	56:97	hepatoprotective and choleretic properties	56:97	Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages.					
36017665	7	68	theme	metabolism	1084:1093	arg1	shift					1066:1070	shift	1066:1070	shift of arginine metabolism to the increase of arginase activity and decrease of NO release	1066:1157	RESULTS The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.					
36017665	1	69	dep	OBJECTIVES	137:146	arg1	developed					287:295	developed	287:295	was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea	283:371	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36017665	1	70	theme	Medical	307:313	arg1	center					315:320	Nargiz Medical center	300:320	Nargiz Medical center (Republic of Azerbaijan)	300:345	OBJECTIVES A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea.					
36332213	10	0	from	agent	1291:1295	arg1	groups					1304:1309	all groups	1300:1309	all groups	1300:1309	The highest bond strength values were obtained after applying the chitosan-containing agent in all groups.					
36332213	3	1	theme	disinfectant	506:517	arg1	application					525:535	disinfectant agent application	506:535	disinfectant agent application (CHX, chitosan, control)	506:560	The teeth were randomly divided into two main groups, treated and/or non-treated with radiotherapy, then separated into three subgroups for disinfectant agent application (CHX, chitosan, control).					
36332213	2	2	used	used	360:363	arg2	teeth					349:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth	281:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth	281:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth were used.					
36332213	3	3	theme	agent	519:523	arg1	application					525:535	disinfectant agent application	506:535	disinfectant agent application (CHX, chitosan, control)	506:560	The teeth were randomly divided into two main groups, treated and/or non-treated with radiotherapy, then separated into three subgroups for disinfectant agent application (CHX, chitosan, control).					
36332213	11	4	theme	positive	1415:1422	arg1	effect					1424:1429	a positive effect	1413:1429	a positive effect	1413:1429	CONCLUSIONS Radiotherapy application had a negative effect, while the use of disinfectant agents had a positive effect on the bond strength.					
36332213	1	5	theme	chitosan-containing	253:271	arg1	gluconate					240:248	Chlorhexidine gluconate	226:248	Chlorhexidine gluconate	226:248	INTRODUCTION This study aimed to compare the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent).					
36332213	1	5	theme	chitosan-containing	253:271	arg1	agent					273:277	a chitosan-containing agent	251:277	a chitosan-containing agent	251:277	INTRODUCTION This study aimed to compare the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent).					
36332213	0	6	from	Effect	0:5	arg1	strength					72:79	dentin bonding strength	57:79	dentin bonding strength after radiotherapy	57:98	Effect of the cavity disinfectant containing chitosan on dentin bonding strength after radiotherapy.					
36332213	8	7	theme	no	970:971	arg1	application					986:996	no radiotherapy application	970:996	no radiotherapy application	970:996	RESULTS Among all groups, Group CH, with no radiotherapy application and containing a chitosan-disinfectant agent, showed the highest bond strength (44.7 ± 8.2).					
36332213	6	8	theme	strength	780:787	arg1	values					789:794	All bond strength values	771:794	All bond strength values from sticks	771:806	All bond strength values from sticks were measured using a universal testing machine.					
36332213	4	9	theme	radiation	625:633	arg1	accelerator					635:645	a linear radiation accelerator	616:645	a linear radiation accelerator for radiotherapy	616:662	A total dose of 70.2 Gy was given over 39 days using a linear radiation accelerator for radiotherapy.					
36332213	9	10	theme	bond	1171:1174	arg1	value					1185:1189	the lowest bond strength value	1160:1189	the lowest bond strength value (29.1 ± 3.5)	1160:1202	In contrast, Group RC with radiotherapy and disinfectant-free showed the lowest bond strength value (29.1 ± 3.5).					
36332213	9	10	theme	bond	1171:1174	arg1	±					1197:1197	29.1 ± 3.5	1192:1201	29.1 ± 3.5	1192:1201	In contrast, Group RC with radiotherapy and disinfectant-free showed the lowest bond strength value (29.1 ± 3.5).					
36332213	6	11	theme	bond	775:778	arg1	values					789:794	All bond strength values	771:794	All bond strength values from sticks	771:806	All bond strength values from sticks were measured using a universal testing machine.					
36332213	11	12	theme	negative	1355:1362	arg1	effect					1364:1369	a negative effect	1353:1369	a negative effect	1353:1369	CONCLUSIONS Radiotherapy application had a negative effect, while the use of disinfectant agents had a positive effect on the bond strength.					
36332213	11	13	theme	CONCLUSIONS	1312:1322	arg1	application					1337:1347	CONCLUSIONS Radiotherapy application	1312:1347	CONCLUSIONS Radiotherapy application	1312:1347	CONCLUSIONS Radiotherapy application had a negative effect, while the use of disinfectant agents had a positive effect on the bond strength.					
36332213	7	14	theme	p	916:916	arg1	&lt					918:920	a p &lt	914:920	a p &lt; 0.05	914:926	The data were analyzed with ANOVA and Chi-square test at a p &lt; 0.05.					
36332213	2	15	theme	METHODOLOGY	281:291	arg1	teeth					349:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth	281:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth	281:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth were used.					
36332213	10	16	theme	bond	1217:1220	arg1	values					1231:1236	The highest bond strength values	1205:1236	The highest bond strength values	1205:1236	The highest bond strength values were obtained after applying the chitosan-containing agent in all groups.					
36332213	1	17	theme	teeth	163:167	arg1	strength					151:158	the bond strength	142:158	the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent)	142:278	INTRODUCTION This study aimed to compare the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent).					
36332213	3	18	theme	main	407:410	arg1	groups					412:417	two main groups	403:417	two main groups	403:417	The teeth were randomly divided into two main groups, treated and/or non-treated with radiotherapy, then separated into three subgroups for disinfectant agent application (CHX, chitosan, control).					
36332213	6	19	from	sticks	801:806	arg1	values					789:794	All bond strength values	771:794	All bond strength values from sticks	771:806	All bond strength values from sticks were measured using a universal testing machine.					
36332213	4	20	theme	Gy	584:585	arg1	dose					571:574	A total dose	563:574	A total dose of 70.2 Gy	563:585	A total dose of 70.2 Gy was given over 39 days using a linear radiation accelerator for radiotherapy.					
36332213	4	21	theme	linear	618:623	arg1	accelerator					635:645	a linear radiation accelerator	616:645	a linear radiation accelerator for radiotherapy	616:662	A total dose of 70.2 Gy was given over 39 days using a linear radiation accelerator for radiotherapy.					
36332213	2	22	theme	human	331:335	arg1	teeth					349:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth	281:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth	281:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth were used.					
36332213	8	23	with	CH	961:962	arg1	application					986:996	no radiotherapy application	970:996	no radiotherapy application	970:996	RESULTS Among all groups, Group CH, with no radiotherapy application and containing a chitosan-disinfectant agent, showed the highest bond strength (44.7 ± 8.2).					
36332213	3	24	theme	treated	420:426	arg1	groups					412:417	two main groups	403:417	two main groups	403:417	The teeth were randomly divided into two main groups, treated and/or non-treated with radiotherapy, then separated into three subgroups for disinfectant agent application (CHX, chitosan, control).					
36332213	8	25	theme	Group	955:959	arg1	CH					961:962	Group CH	955:962	Group CH	955:962	RESULTS Among all groups, Group CH, with no radiotherapy application and containing a chitosan-disinfectant agent, showed the highest bond strength (44.7 ± 8.2).					
36332213	10	26	theme	strength	1222:1229	arg1	values					1231:1236	The highest bond strength values	1205:1236	The highest bond strength values	1205:1236	The highest bond strength values were obtained after applying the chitosan-containing agent in all groups.					
36332213	0	27	theme	cavity	14:19	arg1	chitosan					45:52	the cavity disinfectant containing chitosan	10:52	the cavity disinfectant containing chitosan	10:52	Effect of the cavity disinfectant containing chitosan on dentin bonding strength after radiotherapy.					
36332213	8	28	theme	chitosan-disinfectant	1015:1035	arg1	agent					1037:1041	a chitosan-disinfectant agent	1013:1041	a chitosan-disinfectant agent	1013:1041	RESULTS Among all groups, Group CH, with no radiotherapy application and containing a chitosan-disinfectant agent, showed the highest bond strength (44.7 ± 8.2).					
36332213	7	29	theme	Chi-square	895:904	arg1	test					906:909	Chi-square test	895:909	Chi-square test	895:909	The data were analyzed with ANOVA and Chi-square test at a p &lt; 0.05.					
36332213	3	30	dep	application	525:535	arg1	chitosan					543:550	chitosan	543:550	chitosan	543:550	The teeth were randomly divided into two main groups, treated and/or non-treated with radiotherapy, then separated into three subgroups for disinfectant agent application (CHX, chitosan, control).					
36332213	3	30	dep	application	525:535	arg1	CHX					538:540	CHX	538:540	CHX	538:540	The teeth were randomly divided into two main groups, treated and/or non-treated with radiotherapy, then separated into three subgroups for disinfectant agent application (CHX, chitosan, control).					
36332213	3	30	dep	application	525:535	arg1	control					553:559	control	553:559	control	553:559	The teeth were randomly divided into two main groups, treated and/or non-treated with radiotherapy, then separated into three subgroups for disinfectant agent application (CHX, chitosan, control).					
36332213	8	31	dep	RESULTS	929:935	arg1	showed					1044:1049	showed	1044:1049	showed the highest bond strength (44.7 ± 8.2)	1044:1088	RESULTS Among all groups, Group CH, with no radiotherapy application and containing a chitosan-disinfectant agent, showed the highest bond strength (44.7 ± 8.2).					
36332213	5	32	theme	cavity	684:689	arg1	disinfectant					691:702	the cavity disinfectant	680:702	the cavity disinfectant	680:702	After applying the cavity disinfectant, the teeth were restored with composite resin to obtain the stick.					
36332213	6	33	theme	testing	840:846	arg1	machine					848:854	a universal testing machine	828:854	a universal testing machine	828:854	All bond strength values from sticks were measured using a universal testing machine.					
36332213	2	34	theme	extracted	308:316	arg1	teeth					349:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth	281:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth	281:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth were used.					
36332213	10	35	theme	highest	1209:1215	arg1	values					1231:1236	The highest bond strength values	1205:1236	The highest bond strength values	1205:1236	The highest bond strength values were obtained after applying the chitosan-containing agent in all groups.					
36332213	0	36	theme	containing	34:43	arg1	chitosan					45:52	the cavity disinfectant containing chitosan	10:52	the cavity disinfectant containing chitosan	10:52	Effect of the cavity disinfectant containing chitosan on dentin bonding strength after radiotherapy.					
36332213	6	37	theme	universal	830:838	arg1	machine					848:854	a universal testing machine	828:854	a universal testing machine	828:854	All bond strength values from sticks were measured using a universal testing machine.					
36332213	11	38	theme	agents	1402:1407	arg1	use					1382:1384	the use	1378:1384	the use of disinfectant agents	1378:1407	CONCLUSIONS Radiotherapy application had a negative effect, while the use of disinfectant agents had a positive effect on the bond strength.					
36332213	1	39	theme	cavity	204:209	arg1	disinfectants					211:223	two cavity disinfectants	200:223	two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent)	200:278	INTRODUCTION This study aimed to compare the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent).					
36332213	0	40	theme	disinfectant	21:32	arg1	chitosan					45:52	the cavity disinfectant containing chitosan	10:52	the cavity disinfectant containing chitosan	10:52	Effect of the cavity disinfectant containing chitosan on dentin bonding strength after radiotherapy.					
36332213	4	41	theme	total	565:569	arg1	dose					571:574	A total dose	563:574	A total dose of 70.2 Gy	563:585	A total dose of 70.2 Gy was given over 39 days using a linear radiation accelerator for radiotherapy.					
36332213	9	42	theme	lowest	1164:1169	arg1	value					1185:1189	the lowest bond strength value	1160:1189	the lowest bond strength value (29.1 ± 3.5)	1160:1202	In contrast, Group RC with radiotherapy and disinfectant-free showed the lowest bond strength value (29.1 ± 3.5).					
36332213	9	42	theme	lowest	1164:1169	arg1	±					1197:1197	29.1 ± 3.5	1192:1201	29.1 ± 3.5	1192:1201	In contrast, Group RC with radiotherapy and disinfectant-free showed the lowest bond strength value (29.1 ± 3.5).					
36332213	11	43	theme	Radiotherapy	1324:1335	arg1	application					1337:1347	CONCLUSIONS Radiotherapy application	1312:1347	CONCLUSIONS Radiotherapy application	1312:1347	CONCLUSIONS Radiotherapy application had a negative effect, while the use of disinfectant agents had a positive effect on the bond strength.					
36332213	9	44	with	RC	1110:1111	arg1	radiotherapy					1118:1129	radiotherapy	1118:1129	radiotherapy	1118:1129	In contrast, Group RC with radiotherapy and disinfectant-free showed the lowest bond strength value (29.1 ± 3.5).					
36332213	9	44	with	RC	1110:1111	arg1	disinfectant-free					1135:1151	disinfectant-free	1135:1151	disinfectant-free	1135:1151	In contrast, Group RC with radiotherapy and disinfectant-free showed the lowest bond strength value (29.1 ± 3.5).					
36332213	9	45	theme	Group	1104:1108	arg1	RC					1110:1111	Group RC	1104:1111	Group RC with radiotherapy and disinfectant-free	1104:1151	In contrast, Group RC with radiotherapy and disinfectant-free showed the lowest bond strength value (29.1 ± 3.5).					
36332213	3	46	theme	non-treated	435:445	arg1	groups					412:417	two main groups	403:417	two main groups	403:417	The teeth were randomly divided into two main groups, treated and/or non-treated with radiotherapy, then separated into three subgroups for disinfectant agent application (CHX, chitosan, control).					
36332213	8	47	theme	bond	1063:1066	arg1	strength					1068:1075	the highest bond strength	1051:1075	the highest bond strength (44.7 ± 8.2)	1051:1088	RESULTS Among all groups, Group CH, with no radiotherapy application and containing a chitosan-disinfectant agent, showed the highest bond strength (44.7 ± 8.2).					
36332213	8	47	theme	bond	1063:1066	arg1	±					1083:1083	44.7 ± 8.2	1078:1087	44.7 ± 8.2	1078:1087	RESULTS Among all groups, Group CH, with no radiotherapy application and containing a chitosan-disinfectant agent, showed the highest bond strength (44.7 ± 8.2).					
36332213	0	48	theme	chitosan	45:52	arg1	Effect					0:5	Effect	0:5	Effect of the cavity disinfectant containing chitosan on dentin bonding strength after radiotherapy.	0:99	Effect of the cavity disinfectant containing chitosan on dentin bonding strength after radiotherapy.					
36332213	11	49	theme	disinfectant	1389:1400	arg1	agents					1402:1407	disinfectant agents	1389:1407	disinfectant agents	1389:1407	CONCLUSIONS Radiotherapy application had a negative effect, while the use of disinfectant agents had a positive effect on the bond strength.					
36332213	9	50	theme	strength	1176:1183	arg1	value					1185:1189	the lowest bond strength value	1160:1189	the lowest bond strength value (29.1 ± 3.5)	1160:1202	In contrast, Group RC with radiotherapy and disinfectant-free showed the lowest bond strength value (29.1 ± 3.5).					
36332213	9	50	theme	strength	1176:1183	arg1	±					1197:1197	29.1 ± 3.5	1192:1201	29.1 ± 3.5	1192:1201	In contrast, Group RC with radiotherapy and disinfectant-free showed the lowest bond strength value (29.1 ± 3.5).					
36332213	8	51	theme	radiotherapy	973:984	arg1	application					986:996	no radiotherapy application	970:996	no radiotherapy application	970:996	RESULTS Among all groups, Group CH, with no radiotherapy application and containing a chitosan-disinfectant agent, showed the highest bond strength (44.7 ± 8.2).					
36332213	2	52	dep	extracted	308:316	arg1	non-carious					319:329	non-carious	319:329	non-carious	319:329	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth were used.					
36332213	3	53	with	non-treated	435:445	arg1	radiotherapy					452:463	radiotherapy	452:463	radiotherapy	452:463	The teeth were randomly divided into two main groups, treated and/or non-treated with radiotherapy, then separated into three subgroups for disinfectant agent application (CHX, chitosan, control).					
36332213	11	54	theme	bond	1438:1441	arg1	strength					1443:1450	the bond strength	1434:1450	the bond strength	1434:1450	CONCLUSIONS Radiotherapy application had a negative effect, while the use of disinfectant agents had a positive effect on the bond strength.					
36332213	5	55	theme	composite	734:742	arg1	resin					744:748	composite resin	734:748	composite resin	734:748	After applying the cavity disinfectant, the teeth were restored with composite resin to obtain the stick.					
36332213	2	56	theme	molar	343:347	arg1	teeth					349:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth	281:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth	281:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth were used.					
36332213	8	57	theme	highest	1055:1061	arg1	strength					1068:1075	the highest bond strength	1051:1075	the highest bond strength (44.7 ± 8.2)	1051:1088	RESULTS Among all groups, Group CH, with no radiotherapy application and containing a chitosan-disinfectant agent, showed the highest bond strength (44.7 ± 8.2).					
36332213	8	57	theme	highest	1055:1061	arg1	±					1083:1083	44.7 ± 8.2	1078:1087	44.7 ± 8.2	1078:1087	RESULTS Among all groups, Group CH, with no radiotherapy application and containing a chitosan-disinfectant agent, showed the highest bond strength (44.7 ± 8.2).					
36332213	1	58	dep	disinfectants	211:223	arg1	gluconate					240:248	Chlorhexidine gluconate	226:248	Chlorhexidine gluconate	226:248	INTRODUCTION This study aimed to compare the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent).					
36332213	1	58	dep	disinfectants	211:223	arg1	agent					273:277	a chitosan-containing agent	251:277	a chitosan-containing agent	251:277	INTRODUCTION This study aimed to compare the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent).					
36332213	2	59	theme	third	337:341	arg1	teeth					349:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth	281:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth	281:353	METHODOLOGY Eighteen newly extracted, non-carious human third molar teeth were used.					
36332213	7	60	dep	&lt	918:920	arg1	0.05					923:926	0.05	923:926	0.05	923:926	The data were analyzed with ANOVA and Chi-square test at a p &lt; 0.05.					
36332213	1	61	theme	Chlorhexidine	226:238	arg1	gluconate					240:248	Chlorhexidine gluconate	226:248	Chlorhexidine gluconate	226:248	INTRODUCTION This study aimed to compare the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent).					
36332213	1	61	theme	Chlorhexidine	226:238	arg1	agent					273:277	a chitosan-containing agent	251:277	a chitosan-containing agent	251:277	INTRODUCTION This study aimed to compare the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent).					
36332213	0	62	theme	bonding	64:70	arg1	strength					72:79	dentin bonding strength	57:79	dentin bonding strength after radiotherapy	57:98	Effect of the cavity disinfectant containing chitosan on dentin bonding strength after radiotherapy.					
36332213	11	63	contain	had	1409:1411	arg2	effect					1424:1429	a positive effect	1413:1429	a positive effect	1413:1429	CONCLUSIONS Radiotherapy application had a negative effect, while the use of disinfectant agents had a positive effect on the bond strength.					
36332213	11	63	contain	had	1409:1411	arg1	use					1382:1384	the use	1378:1384	the use of disinfectant agents	1378:1407	CONCLUSIONS Radiotherapy application had a negative effect, while the use of disinfectant agents had a positive effect on the bond strength.					
36332213	10	64	theme	chitosan-containing	1271:1289	arg1	agent					1291:1295	the chitosan-containing agent	1267:1295	the chitosan-containing agent in all groups	1267:1309	The highest bond strength values were obtained after applying the chitosan-containing agent in all groups.					
36332213	1	65	dep	INTRODUCTION	101:112	arg1	aimed					125:129	aimed	125:129	aimed to compare the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent)	125:278	INTRODUCTION This study aimed to compare the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent).					
36332213	11	66	contain	had	1349:1351	arg1	application					1337:1347	CONCLUSIONS Radiotherapy application	1312:1347	CONCLUSIONS Radiotherapy application	1312:1347	CONCLUSIONS Radiotherapy application had a negative effect, while the use of disinfectant agents had a positive effect on the bond strength.					
36332213	11	66	contain	had	1349:1351	arg2	effect					1364:1369	a negative effect	1353:1369	a negative effect	1353:1369	CONCLUSIONS Radiotherapy application had a negative effect, while the use of disinfectant agents had a positive effect on the bond strength.					
36332213	0	67	theme	dentin	57:62	arg1	strength					72:79	dentin bonding strength	57:79	dentin bonding strength after radiotherapy	57:98	Effect of the cavity disinfectant containing chitosan on dentin bonding strength after radiotherapy.					
36332213	1	68	theme	bond	146:149	arg1	strength					151:158	the bond strength	142:158	the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent)	142:278	INTRODUCTION This study aimed to compare the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent).					
36096003	2	0	theme	antioxidant	547:557	arg1	substance					559:567	a new antioxidant substance	541:567	a new antioxidant substance	541:567	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	2	0	theme	antioxidant	547:557	arg1	Q10					579:581	coenzyme Q10	570:581	coenzyme Q10	570:581	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	1	1	theme	pumpkin	156:162	arg1	PJ					171:172	PJ	171:172	PJ	171:172	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	1	1	theme	pumpkin	156:162	arg1	juice					164:168	pumpkin juice	156:168	pumpkin juice (PJ) rich in coenzyme Q10	156:194	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	2	2	theme	antioxidant	769:779	arg1	activity					787:794	antioxidant power activity	769:794	antioxidant power activity	769:794	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	1	3	theme	chemical	221:228	arg1	composition					230:240	the chemical composition	217:240	the chemical composition	217:240	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	0	4	theme	microbiota	66:75	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of chemical composition, antioxidant activity, and gut microbiota	0:75	Evaluation of chemical composition, antioxidant activity, and gut microbiota associated with pumpkin juice fermented by Rhodobacter sphaeroides.					
36096003	1	5	theme	Rhodobacter	314:324	arg1	RPJ					339:341	RPJ	339:341	RPJ	339:341	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	1	5	theme	Rhodobacter	314:324	arg1	sphaeroides					326:336	Rhodobacter sphaeroides	314:336	Rhodobacter sphaeroides (RPJ)	314:342	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	2	6	theme	new	543:545	arg1	substance					559:567	a new antioxidant substance	541:567	a new antioxidant substance	541:567	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	2	6	theme	new	543:545	arg1	Q10					579:581	coenzyme Q10	570:581	coenzyme Q10	570:581	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	4	7	theme	bioactive	1034:1042	arg1	substances					1044:1053	bioactive substances	1034:1053	bioactive substances with antioxidant capacity	1034:1079	Pumpkin juice fermented by Rhodobacter sphaeroides not only produces bioactive substances with antioxidant capacity but can also regulate the gut microbiota of mice.					
36096003	0	8	theme	pumpkin	93:99	arg1	juice					101:105	pumpkin juice	93:105	pumpkin juice fermented by Rhodobacter sphaeroides	93:142	Evaluation of chemical composition, antioxidant activity, and gut microbiota associated with pumpkin juice fermented by Rhodobacter sphaeroides.					
36096003	4	9	theme	mice	1125:1128	arg1	microbiota					1111:1120	the gut microbiota	1103:1120	the gut microbiota of mice	1103:1128	Pumpkin juice fermented by Rhodobacter sphaeroides not only produces bioactive substances with antioxidant capacity but can also regulate the gut microbiota of mice.					
36096003	4	10	dep	produces	1025:1032	arg1	only					1020:1023	only	1020:1023	only	1020:1023	Pumpkin juice fermented by Rhodobacter sphaeroides not only produces bioactive substances with antioxidant capacity but can also regulate the gut microbiota of mice.					
36096003	2	11	theme	total	480:484	arg1	content					492:498	the total sugar content	476:498	the total sugar content	476:498	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	4	12	with	substances	1044:1053	arg1	capacity					1072:1079	antioxidant capacity	1060:1079	antioxidant capacity	1060:1079	Pumpkin juice fermented by Rhodobacter sphaeroides not only produces bioactive substances with antioxidant capacity but can also regulate the gut microbiota of mice.					
36096003	3	13	theme	fermentation	951:962	arg1	48 h					943:946	48 h	943:946	48 h of fermentation	943:962	The gut microbiota of mice shifted by increasing Lactobacillus and Bifidobacterium and protected the gut barrier by reducing Proteobacteria after 48 h of fermentation.					
36096003	4	14	theme	gut	1107:1109	arg1	microbiota					1111:1120	the gut microbiota	1103:1120	the gut microbiota of mice	1103:1128	Pumpkin juice fermented by Rhodobacter sphaeroides not only produces bioactive substances with antioxidant capacity but can also regulate the gut microbiota of mice.					
36096003	1	15	theme	antioxidant	243:253	arg1	activity					255:262	antioxidant activity	243:262	antioxidant activity	243:262	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	2	16	theme	viable	359:364	arg1	bacteria					366:373	viable bacteria	359:373	viable bacteria in the juice	359:386	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	4	17	theme	Pumpkin	965:971	arg1	juice					973:977	Pumpkin juice fermented	965:987	Pumpkin juice fermented by Rhodobacter sphaeroides	965:1014	Pumpkin juice fermented by Rhodobacter sphaeroides not only produces bioactive substances with antioxidant capacity but can also regulate the gut microbiota of mice.					
36096003	2	18	theme	coenzyme	570:577	arg1	substance					559:567	a new antioxidant substance	541:567	a new antioxidant substance	541:567	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	2	18	theme	coenzyme	570:577	arg1	Q10					579:581	coenzyme Q10	570:581	coenzyme Q10	570:581	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	2	19	theme	12.345 mg/L	609:619	arg1	content					598:604	a content	596:604	a content of 12.345 mg/L	596:619	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	2	20	theme	ketones	663:669	arg1	levels					622:627	levels	622:627	levels of volatile alcohols, esters, and ketones	622:669	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	4	21	theme	fermented	979:987	arg1	juice					973:977	Pumpkin juice fermented	965:987	Pumpkin juice fermented by Rhodobacter sphaeroides	965:1014	Pumpkin juice fermented by Rhodobacter sphaeroides not only produces bioactive substances with antioxidant capacity but can also regulate the gut microbiota of mice.					
36096003	1	22	theme	rich	175:178	arg1	PJ					171:172	PJ	171:172	PJ	171:172	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	1	22	theme	rich	175:178	arg1	juice					164:168	pumpkin juice	156:168	pumpkin juice (PJ) rich in coenzyme Q10	156:194	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	0	23	theme	composition	23:33	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of chemical composition, antioxidant activity, and gut microbiota	0:75	Evaluation of chemical composition, antioxidant activity, and gut microbiota associated with pumpkin juice fermented by Rhodobacter sphaeroides.					
36096003	0	24	theme	Rhodobacter	120:130	arg1	sphaeroides					132:142	Rhodobacter sphaeroides	120:142	Rhodobacter sphaeroides	120:142	Evaluation of chemical composition, antioxidant activity, and gut microbiota associated with pumpkin juice fermented by Rhodobacter sphaeroides.					
36096003	0	25	theme	chemical	14:21	arg1	composition					23:33	chemical composition	14:33	chemical composition	14:33	Evaluation of chemical composition, antioxidant activity, and gut microbiota associated with pumpkin juice fermented by Rhodobacter sphaeroides.					
36096003	3	26	theme	gut	898:900	arg1	barrier					902:908	the gut barrier	894:908	the gut barrier	894:908	The gut microbiota of mice shifted by increasing Lactobacillus and Bifidobacterium and protected the gut barrier by reducing Proteobacteria after 48 h of fermentation.					
36096003	2	27	theme	antioxidation	700:712	arg1	ability					714:720	antioxidation ability	700:720	antioxidation ability	700:720	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	1	28	theme	coenzyme	183:190	arg1	Q10					192:194	coenzyme Q10	183:194	coenzyme Q10	183:194	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	0	29	theme	antioxidant	36:46	arg1	activity					48:55	antioxidant activity	36:55	antioxidant activity	36:55	Evaluation of chemical composition, antioxidant activity, and gut microbiota associated with pumpkin juice fermented by Rhodobacter sphaeroides.					
36096003	1	30	theme	gut	269:271	arg1	microbiota					273:282	gut microbiota	269:282	gut microbiota	269:282	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	2	31	from	number	349:354	arg1	juice					382:386	the juice	378:386	the juice	378:386	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	2	32	from	bacteria	366:373	arg1	juice					382:386	the juice	378:386	the juice	378:386	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	3	33	theme	mice	819:822	arg1	microbiota					805:814	The gut microbiota	797:814	The gut microbiota of mice	797:822	The gut microbiota of mice shifted by increasing Lactobacillus and Bifidobacterium and protected the gut barrier by reducing Proteobacteria after 48 h of fermentation.					
36096003	1	34	from	Q10	192:194	arg1	rich					175:178	rich	175:178	rich	175:178	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	2	35	theme	bacteria	366:373	arg1	number					349:354	The number	345:354	The number of viable bacteria in the juice	345:386	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	2	36	theme	fermentation	418:429	arg1	process					431:437	the fermentation process	414:437	the fermentation process	414:437	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	2	37	theme	sugar	486:490	arg1	content					492:498	the total sugar content	476:498	the total sugar content	476:498	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	2	38	theme	volatile	632:639	arg1	alcohols					641:648	volatile alcohols	632:648	volatile alcohols	632:648	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	0	39	theme	activity	48:55	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of chemical composition, antioxidant activity, and gut microbiota	0:75	Evaluation of chemical composition, antioxidant activity, and gut microbiota associated with pumpkin juice fermented by Rhodobacter sphaeroides.					
36096003	1	40	theme	pumpkin	287:293	arg1	juice					295:299	pumpkin juice fermented	287:309	pumpkin juice fermented	287:309	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	2	41	dep	improved	722:729	arg1	reducing					760:767	reducing	760:767	reducing antioxidant power activity	760:794	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	4	42	theme	antioxidant	1060:1070	arg1	capacity					1072:1079	antioxidant capacity	1060:1079	antioxidant capacity	1060:1079	Pumpkin juice fermented by Rhodobacter sphaeroides not only produces bioactive substances with antioxidant capacity but can also regulate the gut microbiota of mice.					
36096003	2	43	theme	ferric	749:754	arg1	ion					756:758	particularly the ferric ion	732:758	particularly the ferric ion	732:758	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	1	44	theme	juice	295:299	arg1	composition					230:240	the chemical composition	217:240	the chemical composition	217:240	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	1	44	theme	juice	295:299	arg1	activity					255:262	antioxidant activity	243:262	antioxidant activity	243:262	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	1	44	theme	juice	295:299	arg1	microbiota					273:282	gut microbiota	269:282	gut microbiota	269:282	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	2	45	from	juice	382:386	arg1	number					349:354	The number	345:354	The number of viable bacteria in the juice	345:386	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	2	46	theme	alcohols	641:648	arg1	levels					622:627	levels	622:627	levels of volatile alcohols, esters, and ketones	622:669	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	0	47	theme	gut	62:64	arg1	microbiota					66:75	gut microbiota	62:75	gut microbiota	62:75	Evaluation of chemical composition, antioxidant activity, and gut microbiota associated with pumpkin juice fermented by Rhodobacter sphaeroides.					
36096003	1	48	from	rich	175:178	arg1	Q10					192:194	coenzyme Q10	183:194	coenzyme Q10	183:194	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	3	49	theme	gut	801:803	arg1	microbiota					805:814	The gut microbiota	797:814	The gut microbiota of mice	797:822	The gut microbiota of mice shifted by increasing Lactobacillus and Bifidobacterium and protected the gut barrier by reducing Proteobacteria after 48 h of fermentation.					
36096003	1	50	theme	fermented	301:309	arg1	juice					295:299	pumpkin juice fermented	287:309	pumpkin juice fermented	287:309	To prepare pumpkin juice (PJ) rich in coenzyme Q10, this study analyzed the chemical composition, antioxidant activity, and gut microbiota of pumpkin juice fermented by Rhodobacter sphaeroides (RPJ).					
36096003	4	51	theme	Rhodobacter	992:1002	arg1	sphaeroides					1004:1014	Rhodobacter sphaeroides	992:1014	Rhodobacter sphaeroides	992:1014	Pumpkin juice fermented by Rhodobacter sphaeroides not only produces bioactive substances with antioxidant capacity but can also regulate the gut microbiota of mice.					
36096003	2	52	theme	esters	651:656	arg1	levels					622:627	levels	622:627	levels of volatile alcohols, esters, and ketones	622:669	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
36096003	2	53	theme	power	781:785	arg1	activity					787:794	antioxidant power activity	769:794	antioxidant power activity	769:794	The number of viable bacteria in the juice reached 2.8 × 108 CFU/mL; the fermentation process mainly consumed glucose and sucrose; the total sugar content was reduced, β-carotene was reduced, and a new antioxidant substance, coenzyme Q10, appeared at a content of 12.345 mg/L; levels of volatile alcohols, esters, and ketones significantly increased; and antioxidation ability improved, particularly the ferric ion reducing antioxidant power activity.					
35240215	8	0	theme	developed	1377:1385	arg1	scaffolds					1394:1402	these recently developed hybrid scaffolds	1362:1402	these recently developed hybrid scaffolds	1362:1402	Notably, these recently developed hybrid scaffolds could be considered for peripheral nerve injury (PNI) regeneration.					
35240215	7	1	theme	nestin	1249:1254	arg1	overexpression					1231:1244	overexpression	1231:1244	overexpression of nestin	1231:1254	Additionally, evaluated of the maturation and neurogenic differentiation of PC12 cells emphasized overexpression of nestin and β-tubulin by Immunocytochemistry staining onto PCL/CA/GNR in comparison to PCL/CA composite.					
35240215	7	1	theme	nestin	1249:1254	arg1	β-tubulin					1260:1268	β-tubulin	1260:1268	β-tubulin	1260:1268	Additionally, evaluated of the maturation and neurogenic differentiation of PC12 cells emphasized overexpression of nestin and β-tubulin by Immunocytochemistry staining onto PCL/CA/GNR in comparison to PCL/CA composite.					
35240215	3	2	theme	scanning	679:686	arg1	microscopy					697:706	scanning electron microscopy	679:706	scanning electron microscopy (SEM)	679:712	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	3	2	theme	scanning	679:686	arg1	SEM					709:711	SEM	709:711	SEM	709:711	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	3	3	theme	electron	688:695	arg1	microscopy					697:706	scanning electron microscopy	679:706	scanning electron microscopy (SEM)	679:712	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	3	3	theme	electron	688:695	arg1	SEM					709:711	SEM	709:711	SEM	709:711	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	4	4	theme	PCL/CA/GNR	844:853	arg1	conductivity					828:839	electrical conductivity	817:839	electrical conductivity of PCL/CA/GNR	817:853	Because of gold nanorods incorporation, electrical conductivity of PCL/CA/GNR increased ~21%.					
35240215	3	5	theme	GNR	559:561	arg1	distribution					563:574	the GNR distribution	555:574	the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio)	555:614	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	8	6	theme	peripheral	1428:1437	arg1	PNI					1453:1455	PNI	1453:1455	PNI	1453:1455	Notably, these recently developed hybrid scaffolds could be considered for peripheral nerve injury (PNI) regeneration.					
35240215	8	6	theme	peripheral	1428:1437	arg1	injury					1445:1450	peripheral nerve injury	1428:1450	peripheral nerve injury (PNI) regeneration	1428:1469	Notably, these recently developed hybrid scaffolds could be considered for peripheral nerve injury (PNI) regeneration.					
35240215	5	7	dep	surface	918:924	arg1	wettable					934:941	wettable	934:941	wettable	934:941	Water contact angle data emphasized PCL/CA/GNR surface is more wettable that PCL/CA (<90° at 62 s).					
35240215	8	8	theme	hybrid	1387:1392	arg1	scaffolds					1394:1402	these recently developed hybrid scaffolds	1362:1402	these recently developed hybrid scaffolds	1362:1402	Notably, these recently developed hybrid scaffolds could be considered for peripheral nerve injury (PNI) regeneration.					
35240215	5	9	dep	PCL/CA	948:953	arg1	62 s					964:967	62 s	964:967	62 s	964:967	Water contact angle data emphasized PCL/CA/GNR surface is more wettable that PCL/CA (<90° at 62 s).					
35240215	3	10	dep	distribution	563:574	arg1	ratio					609:613	3/1 Aspect ratio	598:613	3/1 Aspect ratio	598:613	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	3	10	dep	distribution	563:574	arg1	length					587:592	23 ± 2 nm length	577:592	23 ± 2 nm length	577:592	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	7	11	theme	PC12	1209:1212	arg1	cells					1214:1218	PC12 cells	1209:1218	PC12 cells	1209:1218	Additionally, evaluated of the maturation and neurogenic differentiation of PC12 cells emphasized overexpression of nestin and β-tubulin by Immunocytochemistry staining onto PCL/CA/GNR in comparison to PCL/CA composite.					
35240215	3	12	theme	block-free	726:735	arg1	fibers					748:753	block-free and smooth fibers	726:753	block-free and smooth fibers without perturbation	726:774	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	2	13	dep	nanocomposite	378:390	arg1	PCL/CA/GNR					393:402	PCL/CA/GNR	393:402	PCL/CA/GNR	393:402	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR) were fabricated via electrospinning to improve PC12 cells attachment and growth or scaffold cues.					
35240215	1	14	theme	exclusive	193:201	arg1	potential					203:211	the exclusive potential	189:211	the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs)	189:298	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	7	15	theme	cells	1214:1218	arg1	maturation					1164:1173	maturation	1164:1173	maturation	1164:1173	Additionally, evaluated of the maturation and neurogenic differentiation of PC12 cells emphasized overexpression of nestin and β-tubulin by Immunocytochemistry staining onto PCL/CA/GNR in comparison to PCL/CA composite.					
35240215	7	15	theme	cells	1214:1218	arg1	differentiation					1190:1204	neurogenic differentiation	1179:1204	neurogenic differentiation	1179:1204	Additionally, evaluated of the maturation and neurogenic differentiation of PC12 cells emphasized overexpression of nestin and β-tubulin by Immunocytochemistry staining onto PCL/CA/GNR in comparison to PCL/CA composite.					
35240215	2	16	theme	scaffold	488:495	arg1	cues					497:500	scaffold cues	488:500	scaffold cues	488:500	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR) were fabricated via electrospinning to improve PC12 cells attachment and growth or scaffold cues.					
35240215	7	17	from	PCL/CA/GNR	1307:1316	arg1	comparison					1321:1330	comparison	1321:1330	comparison to PCL/CA composite	1321:1350	Additionally, evaluated of the maturation and neurogenic differentiation of PC12 cells emphasized overexpression of nestin and β-tubulin by Immunocytochemistry staining onto PCL/CA/GNR in comparison to PCL/CA composite.					
35240215	2	18	theme	Gold	301:304	arg1	GNRs					316:319	GNRs	316:319	GNRs	316:319	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR) were fabricated via electrospinning to improve PC12 cells attachment and growth or scaffold cues.					
35240215	2	18	theme	Gold	301:304	arg1	nanorods					306:313	Gold nanorods	301:313	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR)	301:403	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR) were fabricated via electrospinning to improve PC12 cells attachment and growth or scaffold cues.					
35240215	5	19	theme	contact	877:883	arg1	data					891:894	Water contact angle data	871:894	Water contact angle data	871:894	Water contact angle data emphasized PCL/CA/GNR surface is more wettable that PCL/CA (<90° at 62 s).					
35240215	7	20	theme	PCL/CA	1335:1340	arg1	composite					1342:1350	PCL/CA composite	1335:1350	PCL/CA composite	1335:1350	Additionally, evaluated of the maturation and neurogenic differentiation of PC12 cells emphasized overexpression of nestin and β-tubulin by Immunocytochemistry staining onto PCL/CA/GNR in comparison to PCL/CA composite.					
35240215	1	21	theme	Synthetic	105:113	arg1	polymers					127:134	Synthetic and natural polymers	105:134	Synthetic and natural polymers	105:134	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	5	22	theme	angle	885:889	arg1	data					891:894	Water contact angle data	871:894	Water contact angle data	871:894	Water contact angle data emphasized PCL/CA/GNR surface is more wettable that PCL/CA (<90° at 62 s).					
35240215	0	23	theme	Gold	0:3	arg1	nanorods					5:12	Gold nanorods	0:12	Gold nanorods	0:12	Gold nanorods decorated polycaprolactone/cellulose acetate hybrid scaffold for PC12 cells proliferation.					
35240215	6	24	theme	PCR	981:983	arg1	RT-PCR					996:1001	RT-PCR	996:1001	RT-PCR	996:1001	Real-time PCR technique (RT-PCR) demonstrated overexpression of β-tubulin and microtubule-associated protein 2 (MAP2) on PCL/CA/GNR compared to PCL/CA composite.					
35240215	6	24	theme	PCR	981:983	arg1	technique					985:993	Real-time PCR technique	971:993	Real-time PCR technique (RT-PCR)	971:1002	Real-time PCR technique (RT-PCR) demonstrated overexpression of β-tubulin and microtubule-associated protein 2 (MAP2) on PCL/CA/GNR compared to PCL/CA composite.					
35240215	2	25	dep	cells	457:461	arg1	cells					457:461	PC12 cells attachment and growth	452:483	PC12 cells attachment and growth	452:483	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR) were fabricated via electrospinning to improve PC12 cells attachment and growth or scaffold cues.					
35240215	2	25	dep	cells	457:461	arg1	growth					478:483	growth	478:483	growth	478:483	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR) were fabricated via electrospinning to improve PC12 cells attachment and growth or scaffold cues.					
35240215	2	25	dep	cells	457:461	arg1	attachment					463:472	attachment	463:472	attachment	463:472	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR) were fabricated via electrospinning to improve PC12 cells attachment and growth or scaffold cues.					
35240215	5	26	theme	Water	871:875	arg1	data					891:894	Water contact angle data	871:894	Water contact angle data	871:894	Water contact angle data emphasized PCL/CA/GNR surface is more wettable that PCL/CA (<90° at 62 s).					
35240215	0	27	theme	polycaprolactone/cellulose	24:49	arg1	scaffold					66:73	polycaprolactone/cellulose acetate hybrid scaffold	24:73	polycaprolactone/cellulose acetate hybrid scaffold for PC12 cells proliferation	24:102	Gold nanorods decorated polycaprolactone/cellulose acetate hybrid scaffold for PC12 cells proliferation.					
35240215	6	28	theme	Real-time	971:979	arg1	RT-PCR					996:1001	RT-PCR	996:1001	RT-PCR	996:1001	Real-time PCR technique (RT-PCR) demonstrated overexpression of β-tubulin and microtubule-associated protein 2 (MAP2) on PCL/CA/GNR compared to PCL/CA composite.					
35240215	6	28	theme	Real-time	971:979	arg1	technique					985:993	Real-time PCR technique	971:993	Real-time PCR technique (RT-PCR)	971:1002	Real-time PCR technique (RT-PCR) demonstrated overexpression of β-tubulin and microtubule-associated protein 2 (MAP2) on PCL/CA/GNR compared to PCL/CA composite.					
35240215	1	29	theme	natural	119:125	arg1	polymers					127:134	Synthetic and natural polymers	105:134	Synthetic and natural polymers	105:134	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	7	30	theme	neurogenic	1179:1188	arg1	differentiation					1190:1204	neurogenic differentiation	1179:1204	neurogenic differentiation	1179:1204	Additionally, evaluated of the maturation and neurogenic differentiation of PC12 cells emphasized overexpression of nestin and β-tubulin by Immunocytochemistry staining onto PCL/CA/GNR in comparison to PCL/CA composite.					
35240215	8	31	theme	injury	1445:1450	arg1	regeneration					1458:1469	peripheral nerve injury (PNI) regeneration	1428:1469	peripheral nerve injury (PNI) regeneration	1428:1469	Notably, these recently developed hybrid scaffolds could be considered for peripheral nerve injury (PNI) regeneration.					
35240215	3	32	theme	23 ± 2 nm	577:585	arg1	length					587:592	23 ± 2 nm length	577:592	23 ± 2 nm length	577:592	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	1	33	theme	regenerative	232:243	arg1	processes					254:262	the regenerative cellular processes	228:262	the regenerative cellular processes in peripheral nerve injuries (PNIs)	228:298	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	0	34	theme	hybrid	59:64	arg1	scaffold					66:73	polycaprolactone/cellulose acetate hybrid scaffold	24:73	polycaprolactone/cellulose acetate hybrid scaffold for PC12 cells proliferation	24:102	Gold nanorods decorated polycaprolactone/cellulose acetate hybrid scaffold for PC12 cells proliferation.					
35240215	3	35	theme	smooth	741:746	arg1	fibers					748:753	block-free and smooth fibers	726:753	block-free and smooth fibers without perturbation	726:774	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	6	36	theme	microtubule-associated	1049:1070	arg1	protein					1072:1078	microtubule-associated protein 2	1049:1080	microtubule-associated protein 2 (MAP2)	1049:1087	Real-time PCR technique (RT-PCR) demonstrated overexpression of β-tubulin and microtubule-associated protein 2 (MAP2) on PCL/CA/GNR compared to PCL/CA composite.					
35240215	6	36	theme	microtubule-associated	1049:1070	arg1	MAP2					1083:1086	MAP2	1083:1086	MAP2	1083:1086	Real-time PCR technique (RT-PCR) demonstrated overexpression of β-tubulin and microtubule-associated protein 2 (MAP2) on PCL/CA/GNR compared to PCL/CA composite.					
35240215	1	37	theme	cellular	245:252	arg1	processes					254:262	the regenerative cellular processes	228:262	the regenerative cellular processes in peripheral nerve injuries (PNIs)	228:298	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	0	38	theme	acetate	51:57	arg1	scaffold					66:73	polycaprolactone/cellulose acetate hybrid scaffold	24:73	polycaprolactone/cellulose acetate hybrid scaffold for PC12 cells proliferation	24:102	Gold nanorods decorated polycaprolactone/cellulose acetate hybrid scaffold for PC12 cells proliferation.					
35240215	2	39	theme	PC12	452:455	arg1	cells					457:461	PC12 cells attachment and growth	452:483	PC12 cells attachment and growth	452:483	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR) were fabricated via electrospinning to improve PC12 cells attachment and growth or scaffold cues.					
35240215	2	39	theme	PC12	452:455	arg1	growth					478:483	growth	478:483	growth	478:483	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR) were fabricated via electrospinning to improve PC12 cells attachment and growth or scaffold cues.					
35240215	2	39	theme	PC12	452:455	arg1	attachment					463:472	attachment	463:472	attachment	463:472	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR) were fabricated via electrospinning to improve PC12 cells attachment and growth or scaffold cues.					
35240215	6	40	theme	PCL/CA	1115:1120	arg1	composite					1122:1130	PCL/CA composite	1115:1130	PCL/CA composite	1115:1130	Real-time PCR technique (RT-PCR) demonstrated overexpression of β-tubulin and microtubule-associated protein 2 (MAP2) on PCL/CA/GNR compared to PCL/CA composite.					
35240215	3	41	theme	800 nm	650:655	arg1	distribution					563:574	the GNR distribution	555:574	the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio)	555:614	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	3	41	theme	800 nm	650:655	arg1	dimension					637:645	suitable average dimension	620:645	suitable average dimension	620:645	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	3	42	theme	3/1	598:600	arg1	ratio					609:613	3/1 Aspect ratio	598:613	3/1 Aspect ratio	598:613	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	1	43	from	processes	254:262	arg1	PNIs					294:297	PNIs	294:297	PNIs	294:297	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	1	43	from	processes	254:262	arg1	injuries					284:291	peripheral nerve injuries	267:291	peripheral nerve injuries (PNIs)	267:298	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	3	44	theme	Transmission	503:514	arg1	TEM					537:539	TEM	537:539	TEM	537:539	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	3	44	theme	Transmission	503:514	arg1	microscopy					525:534	Transmission electron microscopy	503:534	Transmission electron microscopy (TEM)	503:540	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	3	45	theme	Aspect	602:607	arg1	ratio					609:613	3/1 Aspect ratio	598:613	3/1 Aspect ratio	598:613	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	3	46	theme	electron	516:523	arg1	TEM					537:539	TEM	537:539	TEM	537:539	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	3	46	theme	electron	516:523	arg1	microscopy					525:534	Transmission electron microscopy	503:534	Transmission electron microscopy (TEM)	503:540	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	4	47	theme	nanorods	793:800	arg1	incorporation					802:814	gold nanorods incorporation	788:814	gold nanorods incorporation	788:814	Because of gold nanorods incorporation, electrical conductivity of PCL/CA/GNR increased ~21%.					
35240215	3	48	theme	average	629:635	arg1	dimension					637:645	suitable average dimension	620:645	suitable average dimension	620:645	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	4	49	theme	electrical	817:826	arg1	conductivity					828:839	electrical conductivity	817:839	electrical conductivity of PCL/CA/GNR	817:853	Because of gold nanorods incorporation, electrical conductivity of PCL/CA/GNR increased ~21%.					
35240215	8	50	theme	nerve	1439:1443	arg1	PNI					1453:1455	PNI	1453:1455	PNI	1453:1455	Notably, these recently developed hybrid scaffolds could be considered for peripheral nerve injury (PNI) regeneration.					
35240215	8	50	theme	nerve	1439:1443	arg1	injury					1445:1450	peripheral nerve injury	1428:1450	peripheral nerve injury (PNI) regeneration	1428:1469	Notably, these recently developed hybrid scaffolds could be considered for peripheral nerve injury (PNI) regeneration.					
35240215	6	51	theme	β-tubulin	1035:1043	arg1	protein					1072:1078	microtubule-associated protein 2	1049:1080	microtubule-associated protein 2 (MAP2)	1049:1087	Real-time PCR technique (RT-PCR) demonstrated overexpression of β-tubulin and microtubule-associated protein 2 (MAP2) on PCL/CA/GNR compared to PCL/CA composite.					
35240215	6	51	theme	β-tubulin	1035:1043	arg1	overexpression					1017:1030	overexpression	1017:1030	overexpression of β-tubulin	1017:1043	Real-time PCR technique (RT-PCR) demonstrated overexpression of β-tubulin and microtubule-associated protein 2 (MAP2) on PCL/CA/GNR compared to PCL/CA composite.					
35240215	6	51	theme	β-tubulin	1035:1043	arg1	MAP2					1083:1086	MAP2	1083:1086	MAP2	1083:1086	Real-time PCR technique (RT-PCR) demonstrated overexpression of β-tubulin and microtubule-associated protein 2 (MAP2) on PCL/CA/GNR compared to PCL/CA composite.					
35240215	4	52	theme	gold	788:791	arg1	incorporation					802:814	gold nanorods incorporation	788:814	gold nanorods incorporation	788:814	Because of gold nanorods incorporation, electrical conductivity of PCL/CA/GNR increased ~21%.					
35240215	1	53	theme	considerable	159:170	arg1	attention					172:180	considerable attention	159:180	considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs)	159:298	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	1	54	theme	peripheral	267:276	arg1	PNIs					294:297	PNIs	294:297	PNIs	294:297	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	1	54	theme	peripheral	267:276	arg1	injuries					284:291	peripheral nerve injuries	267:291	peripheral nerve injuries (PNIs)	267:298	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	0	55	theme	cells	84:88	arg1	proliferation					90:102	PC12 cells proliferation	79:102	PC12 cells proliferation	79:102	Gold nanorods decorated polycaprolactone/cellulose acetate hybrid scaffold for PC12 cells proliferation.					
35240215	5	56	theme	PCL/CA/GNR	907:916	arg1	surface					918:924	PCL/CA/GNR surface	907:924	PCL/CA/GNR surface is more wettable that PCL/CA (<90° at 62 s)	907:968	Water contact angle data emphasized PCL/CA/GNR surface is more wettable that PCL/CA (<90° at 62 s).					
35240215	2	57	theme	nanocomposite	378:390	arg1	GNRs					316:319	GNRs	316:319	GNRs	316:319	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR) were fabricated via electrospinning to improve PC12 cells attachment and growth or scaffold cues.					
35240215	2	57	theme	nanocomposite	378:390	arg1	nanorods					306:313	Gold nanorods	301:313	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR)	301:403	Gold nanorods (GNRs) decorated polycaprolactone (PCL)/cellulose acetate (CA) nanocomposite (PCL/CA/GNR) were fabricated via electrospinning to improve PC12 cells attachment and growth or scaffold cues.					
35240215	1	58	theme	nerve	278:282	arg1	PNIs					294:297	PNIs	294:297	PNIs	294:297	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	1	58	theme	nerve	278:282	arg1	injuries					284:291	peripheral nerve injuries	267:291	peripheral nerve injuries (PNIs)	267:298	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	0	59	theme	PC12	79:82	arg1	cells					84:88	PC12 cells	79:88	PC12 cells proliferation	79:102	Gold nanorods decorated polycaprolactone/cellulose acetate hybrid scaffold for PC12 cells proliferation.					
35240215	3	60	theme	suitable	620:627	arg1	dimension					637:645	suitable average dimension	620:645	suitable average dimension	620:645	Transmission electron microscopy (TEM) corroborated the GNR distribution (23 ± 2 nm length and 3/1 Aspect ratio) and suitable average dimension of 800 nm for the fibers; also, scanning electron microscopy (SEM) represented block-free and smooth fibers without perturbation.					
35240215	1	61	theme	due	182:184	arg1	attention					172:180	considerable attention	159:180	considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs)	159:298	Synthetic and natural polymers have recently received considerable attention due to the exclusive potential for supporting the regenerative cellular processes in peripheral nerve injuries (PNIs).					
35240215	7	62	dep	maturation	1164:1173	arg1	the					1160:1162	the	1160:1162	the	1160:1162	Additionally, evaluated of the maturation and neurogenic differentiation of PC12 cells emphasized overexpression of nestin and β-tubulin by Immunocytochemistry staining onto PCL/CA/GNR in comparison to PCL/CA composite.					
35483003	0	0	with	Gel	28:30	arg1	Self-Healability					72:87	Self-Healability	72:87	Self-Healability	72:87	A Chewing Gum Residue-Based Gel with Superior Mechanical Properties and Self-Healability for Flexible Wearable Sensor.					
35483003	0	0	with	Gel	28:30	arg1	Properties					57:66	Superior Mechanical Properties	37:66	Superior Mechanical Properties	37:66	A Chewing Gum Residue-Based Gel with Superior Mechanical Properties and Self-Healability for Flexible Wearable Sensor.					
35483003	6	1	theme	human	943:947	arg1	motion					949:954	human motion	943:954	human motion	943:954	In particular, the gel with a fast signal response is successfully applied as a wearable strain sensor to monitor different types of human motion.					
35483003	5	2	from	break	697:701	arg1	performance					771:781	excellent self-healing performance	748:781	excellent self-healing performance	748:781	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	5	2	from	break	697:701	arg1	elongation					683:692	high elongation	678:692	high elongation at break of 610% and strength of 0.11 MPa	678:734	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	5	2	from	break	697:701	arg1	properties					798:807	recyclable properties	787:807	recyclable properties	787:807	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	10	3	theme	obtained	1333:1340	arg1	candidate					1365:1373	a promising candidate	1353:1373	a promising candidate for the fabrication of flexible wearable sensor	1353:1421	The obtained gel may be a promising candidate for the fabrication of flexible wearable sensor.					
35483003	10	3	theme	obtained	1333:1340	arg1	gel					1342:1344	The obtained gel	1329:1344	The obtained gel	1329:1344	The obtained gel may be a promising candidate for the fabrication of flexible wearable sensor.					
35483003	2	4	with	gel	266:268	arg1	properties					295:304	enhanced mechanical properties	275:304	enhanced mechanical properties	275:304	In this paper, a chewing gum gel with enhanced mechanical properties and self-healing properties is prepared by using polyvinyl alcohol (PVA) as the backbone in chewing gum residue.					
35483003	2	4	with	gel	266:268	arg1	properties					323:332	self-healing properties	310:332	self-healing properties	310:332	In this paper, a chewing gum gel with enhanced mechanical properties and self-healing properties is prepared by using polyvinyl alcohol (PVA) as the backbone in chewing gum residue.					
35483003	0	5	theme	Wearable	102:109	arg1	Sensor					111:116	Flexible Wearable Sensor	93:116	Flexible Wearable Sensor	93:116	A Chewing Gum Residue-Based Gel with Superior Mechanical Properties and Self-Healability for Flexible Wearable Sensor.					
35483003	7	6	theme	linear	1070:1075	arg1	sensitivity					1077:1087	wide linear sensitivity	1065:1087	wide linear sensitivity	1065:1087	The gel as a sensor exhibits self-healing properties suggesting superior safety and stability, and displays wide linear sensitivity (the gauge factor is 0.417 and 0.170).					
35483003	2	7	theme	gum	262:264	arg1	gel					266:268	a chewing gum gel	252:268	a chewing gum gel with enhanced mechanical properties and self-healing properties	252:332	In this paper, a chewing gum gel with enhanced mechanical properties and self-healing properties is prepared by using polyvinyl alcohol (PVA) as the backbone in chewing gum residue.					
35483003	6	8	theme	motion	949:954	arg1	types					934:938	different types	924:938	different types of human motion	924:954	In particular, the gel with a fast signal response is successfully applied as a wearable strain sensor to monitor different types of human motion.					
35483003	0	9	theme	Flexible	93:100	arg1	Sensor					111:116	Flexible Wearable Sensor	93:116	Flexible Wearable Sensor	93:116	A Chewing Gum Residue-Based Gel with Superior Mechanical Properties and Self-Healability for Flexible Wearable Sensor.					
35483003	10	10	theme	promising	1355:1363	arg1	candidate					1365:1373	a promising candidate	1353:1373	a promising candidate for the fabrication of flexible wearable sensor	1353:1421	The obtained gel may be a promising candidate for the fabrication of flexible wearable sensor.					
35483003	10	10	theme	promising	1355:1363	arg1	gel					1342:1344	The obtained gel	1329:1344	The obtained gel	1329:1344	The obtained gel may be a promising candidate for the fabrication of flexible wearable sensor.					
35483003	2	11	theme	chewing	254:260	arg1	gel					266:268	a chewing gum gel	252:268	a chewing gum gel with enhanced mechanical properties and self-healing properties	252:332	In this paper, a chewing gum gel with enhanced mechanical properties and self-healing properties is prepared by using polyvinyl alcohol (PVA) as the backbone in chewing gum residue.					
35483003	4	12	theme	physical	552:559	arg1	crosslinking					561:572	The physical crosslinking	548:572	The physical crosslinking	548:572	The physical crosslinking is realized by further freeze-thaw treatment to improve its mechanical properties.					
35483003	3	13	theme	borax	445:449	arg1	bond					457:460	the borax ester bond	441:460	the borax ester bond	441:460	The hydrogen bond and the borax ester bond are employed to construct reversible interaction to enhance the self-healing ability.					
35483003	10	14	theme	wearable	1407:1414	arg1	sensor					1416:1421	flexible wearable sensor	1398:1421	flexible wearable sensor	1398:1421	The obtained gel may be a promising candidate for the fabrication of flexible wearable sensor.					
35483003	4	15	theme	mechanical	634:643	arg1	properties					645:654	its mechanical properties	630:654	its mechanical properties	630:654	The physical crosslinking is realized by further freeze-thaw treatment to improve its mechanical properties.					
35483003	5	16	theme	strength	715:722	arg1	performance					771:781	excellent self-healing performance	748:781	excellent self-healing performance	748:781	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	5	16	theme	strength	715:722	arg1	elongation					683:692	high elongation	678:692	high elongation at break of 610% and strength of 0.11 MPa	678:734	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	5	16	theme	strength	715:722	arg1	properties					798:807	recyclable properties	787:807	recyclable properties	787:807	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	6	17	theme	signal	845:850	arg1	response					852:859	a fast signal response	838:859	a fast signal response	838:859	In particular, the gel with a fast signal response is successfully applied as a wearable strain sensor to monitor different types of human motion.					
35483003	7	18	theme	wide	1065:1068	arg1	sensitivity					1077:1087	wide linear sensitivity	1065:1087	wide linear sensitivity	1065:1087	The gel as a sensor exhibits self-healing properties suggesting superior safety and stability, and displays wide linear sensitivity (the gauge factor is 0.417 and 0.170).					
35483003	10	19	theme	sensor	1416:1421	arg1	fabrication					1383:1393	the fabrication	1379:1393	the fabrication of flexible wearable sensor	1379:1421	The obtained gel may be a promising candidate for the fabrication of flexible wearable sensor.					
35483003	3	20	theme	reversible	488:497	arg1	interaction					499:509	reversible interaction	488:509	reversible interaction	488:509	The hydrogen bond and the borax ester bond are employed to construct reversible interaction to enhance the self-healing ability.					
35483003	8	21	theme	temperature	1169:1179	arg1	changes					1181:1187	temperature changes	1169:1187	temperature changes	1169:1187	The gel can be further served to explore temperature changes, implying the application in temperature monitoring.					
35483003	0	22	theme	Chewing	2:8	arg1	Gel					28:30	A Chewing Gum Residue-Based Gel	0:30	A Chewing Gum Residue-Based Gel with Superior Mechanical Properties and Self-Healability for Flexible Wearable Sensor.	0:117	A Chewing Gum Residue-Based Gel with Superior Mechanical Properties and Self-Healability for Flexible Wearable Sensor.					
35483003	6	23	theme	fast	840:843	arg1	response					852:859	a fast signal response	838:859	a fast signal response	838:859	In particular, the gel with a fast signal response is successfully applied as a wearable strain sensor to monitor different types of human motion.					
35483003	2	24	theme	mechanical	284:293	arg1	properties					295:304	enhanced mechanical properties	275:304	enhanced mechanical properties	275:304	In this paper, a chewing gum gel with enhanced mechanical properties and self-healing properties is prepared by using polyvinyl alcohol (PVA) as the backbone in chewing gum residue.					
35483003	2	25	theme	gum	406:408	arg1	residue					410:416	gum residue	406:416	gum residue	406:416	In this paper, a chewing gum gel with enhanced mechanical properties and self-healing properties is prepared by using polyvinyl alcohol (PVA) as the backbone in chewing gum residue.					
35483003	9	26	theme	chewing	1308:1314	arg1	residue					1320:1326	chewing gum residue	1308:1326	chewing gum residue	1308:1326	This study develops a novel approach for the recycle and reuse of chewing gum residue.					
35483003	2	27	theme	enhanced	275:282	arg1	properties					295:304	enhanced mechanical properties	275:304	enhanced mechanical properties	275:304	In this paper, a chewing gum gel with enhanced mechanical properties and self-healing properties is prepared by using polyvinyl alcohol (PVA) as the backbone in chewing gum residue.					
35483003	2	28	theme	polyvinyl	355:363	arg1	PVA					374:376	PVA	374:376	PVA	374:376	In this paper, a chewing gum gel with enhanced mechanical properties and self-healing properties is prepared by using polyvinyl alcohol (PVA) as the backbone in chewing gum residue.					
35483003	2	28	theme	polyvinyl	355:363	arg1	alcohol					365:371	polyvinyl alcohol	355:371	polyvinyl alcohol (PVA)	355:377	In this paper, a chewing gum gel with enhanced mechanical properties and self-healing properties is prepared by using polyvinyl alcohol (PVA) as the backbone in chewing gum residue.					
35483003	2	28	theme	polyvinyl	355:363	arg1	backbone					386:393	the backbone	382:393	the backbone in chewing gum residue	382:416	In this paper, a chewing gum gel with enhanced mechanical properties and self-healing properties is prepared by using polyvinyl alcohol (PVA) as the backbone in chewing gum residue.					
35483003	0	29	theme	Residue-Based	14:26	arg1	Gel					28:30	A Chewing Gum Residue-Based Gel	0:30	A Chewing Gum Residue-Based Gel with Superior Mechanical Properties and Self-Healability for Flexible Wearable Sensor.	0:117	A Chewing Gum Residue-Based Gel with Superior Mechanical Properties and Self-Healability for Flexible Wearable Sensor.					
35483003	5	30	theme	excellent	748:756	arg1	performance					771:781	excellent self-healing performance	748:781	excellent self-healing performance	748:781	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	3	31	theme	hydrogen	423:430	arg1	bond					432:435	The hydrogen bond	419:435	The hydrogen bond	419:435	The hydrogen bond and the borax ester bond are employed to construct reversible interaction to enhance the self-healing ability.					
35483003	0	32	theme	Gum	10:12	arg1	Gel					28:30	A Chewing Gum Residue-Based Gel	0:30	A Chewing Gum Residue-Based Gel with Superior Mechanical Properties and Self-Healability for Flexible Wearable Sensor.	0:117	A Chewing Gum Residue-Based Gel with Superior Mechanical Properties and Self-Healability for Flexible Wearable Sensor.					
35483003	6	33	theme	different	924:932	arg1	types					934:938	different types	924:938	different types of human motion	924:954	In particular, the gel with a fast signal response is successfully applied as a wearable strain sensor to monitor different types of human motion.					
35483003	7	34	theme	self-healing	986:997	arg1	properties					999:1008	self-healing properties	986:1008	self-healing properties suggesting superior safety and stability	986:1049	The gel as a sensor exhibits self-healing properties suggesting superior safety and stability, and displays wide linear sensitivity (the gauge factor is 0.417 and 0.170).					
35483003	4	35	theme	freeze-thaw	597:607	arg1	treatment					609:617	further freeze-thaw treatment	589:617	further freeze-thaw treatment	589:617	The physical crosslinking is realized by further freeze-thaw treatment to improve its mechanical properties.					
35483003	1	36	theme	gum	127:129	arg1	residue					131:137	Chewing gum residue	119:137	Chewing gum residue	119:137	Chewing gum residue is hard to decompose and easy to cause pollution, which is highly desirable to realize recycling.					
35483003	7	37	theme	superior	1021:1028	arg1	safety					1030:1035	superior safety	1021:1035	superior safety	1021:1035	The gel as a sensor exhibits self-healing properties suggesting superior safety and stability, and displays wide linear sensitivity (the gauge factor is 0.417 and 0.170).					
35483003	8	38	theme	temperature	1218:1228	arg1	monitoring					1230:1239	temperature monitoring	1218:1239	temperature monitoring	1218:1239	The gel can be further served to explore temperature changes, implying the application in temperature monitoring.					
35483003	7	39	theme	gauge	1094:1098	arg1	0.417					1110:1114	0.417	1110:1114	0.417	1110:1114	The gel as a sensor exhibits self-healing properties suggesting superior safety and stability, and displays wide linear sensitivity (the gauge factor is 0.417 and 0.170).					
35483003	7	39	theme	gauge	1094:1098	arg1	factor					1100:1105	the gauge factor	1090:1105	the gauge factor	1090:1105	The gel as a sensor exhibits self-healing properties suggesting superior safety and stability, and displays wide linear sensitivity (the gauge factor is 0.417 and 0.170).					
35483003	6	40	with	gel	829:831	arg1	response					852:859	a fast signal response	838:859	a fast signal response	838:859	In particular, the gel with a fast signal response is successfully applied as a wearable strain sensor to monitor different types of human motion.					
35483003	9	41	theme	gum	1316:1318	arg1	residue					1320:1326	chewing gum residue	1308:1326	chewing gum residue	1308:1326	This study develops a novel approach for the recycle and reuse of chewing gum residue.					
35483003	5	42	theme	recyclable	787:796	arg1	properties					798:807	recyclable properties	787:807	recyclable properties	787:807	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	6	43	theme	wearable	890:897	arg1	sensor					906:911	a wearable strain sensor	888:911	a wearable strain sensor	888:911	In particular, the gel with a fast signal response is successfully applied as a wearable strain sensor to monitor different types of human motion.					
35483003	6	43	theme	wearable	890:897	arg1	gel					829:831	the gel	825:831	the gel with a fast signal response	825:859	In particular, the gel with a fast signal response is successfully applied as a wearable strain sensor to monitor different types of human motion.					
35483003	0	44	theme	Mechanical	46:55	arg1	Properties					57:66	Superior Mechanical Properties	37:66	Superior Mechanical Properties	37:66	A Chewing Gum Residue-Based Gel with Superior Mechanical Properties and Self-Healability for Flexible Wearable Sensor.					
35483003	5	45	theme	self-healing	758:769	arg1	performance					771:781	excellent self-healing performance	748:781	excellent self-healing performance	748:781	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	9	46	theme	novel	1264:1268	arg1	approach					1270:1277	a novel approach	1262:1277	a novel approach for the recycle and reuse of chewing gum residue	1262:1326	This study develops a novel approach for the recycle and reuse of chewing gum residue.					
35483003	1	47	theme	Chewing	119:125	arg1	residue					131:137	Chewing gum residue	119:137	Chewing gum residue	119:137	Chewing gum residue is hard to decompose and easy to cause pollution, which is highly desirable to realize recycling.					
35483003	0	48	theme	Superior	37:44	arg1	Properties					57:66	Superior Mechanical Properties	37:66	Superior Mechanical Properties	37:66	A Chewing Gum Residue-Based Gel with Superior Mechanical Properties and Self-Healability for Flexible Wearable Sensor.					
35483003	6	49	theme	strain	899:904	arg1	sensor					906:911	a wearable strain sensor	888:911	a wearable strain sensor	888:911	In particular, the gel with a fast signal response is successfully applied as a wearable strain sensor to monitor different types of human motion.					
35483003	6	49	theme	strain	899:904	arg1	gel					829:831	the gel	825:831	the gel with a fast signal response	825:859	In particular, the gel with a fast signal response is successfully applied as a wearable strain sensor to monitor different types of human motion.					
35483003	10	50	theme	flexible	1398:1405	arg1	sensor					1416:1421	flexible wearable sensor	1398:1421	flexible wearable sensor	1398:1421	The obtained gel may be a promising candidate for the fabrication of flexible wearable sensor.					
35483003	3	51	theme	ester	451:455	arg1	bond					457:460	the borax ester bond	441:460	the borax ester bond	441:460	The hydrogen bond and the borax ester bond are employed to construct reversible interaction to enhance the self-healing ability.					
35483003	3	52	theme	self-healing	526:537	arg1	ability					539:545	the self-healing ability	522:545	the self-healing ability	522:545	The hydrogen bond and the borax ester bond are employed to construct reversible interaction to enhance the self-healing ability.					
35483003	9	53	theme	residue	1320:1326	arg1	recycle					1287:1293	recycle	1287:1293	recycle	1287:1293	This study develops a novel approach for the recycle and reuse of chewing gum residue.					
35483003	9	53	theme	residue	1320:1326	arg1	reuse					1299:1303	reuse	1299:1303	reuse	1299:1303	This study develops a novel approach for the recycle and reuse of chewing gum residue.					
35483003	5	54	theme	high	678:681	arg1	elongation					683:692	high elongation	678:692	high elongation at break of 610% and strength of 0.11 MPa	678:734	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	2	55	theme	self-healing	310:321	arg1	properties					323:332	self-healing properties	310:332	self-healing properties	310:332	In this paper, a chewing gum gel with enhanced mechanical properties and self-healing properties is prepared by using polyvinyl alcohol (PVA) as the backbone in chewing gum residue.					
35483003	4	56	theme	further	589:595	arg1	treatment					609:617	further freeze-thaw treatment	589:617	further freeze-thaw treatment	589:617	The physical crosslinking is realized by further freeze-thaw treatment to improve its mechanical properties.					
35483003	5	57	theme	0.11 MPa	727:734	arg1	0.11 MPa					727:734	0.11 MPa	727:734	0.11 MPa	727:734	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	5	57	theme	0.11 MPa	727:734	arg1	strength					715:722	strength	715:722	strength	715:722	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	5	57	theme	0.11 MPa	727:734	arg1	%					709:709	610%	706:709	610%	706:709	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	9	58	dep	recycle	1287:1293	arg1	the					1283:1285	the	1283:1285	the	1283:1285	This study develops a novel approach for the recycle and reuse of chewing gum residue.					
35483003	8	59	from	application	1203:1213	arg1	monitoring					1230:1239	temperature monitoring	1218:1239	temperature monitoring	1218:1239	The gel can be further served to explore temperature changes, implying the application in temperature monitoring.					
35483003	5	60	theme	%	709:709	arg1	performance					771:781	excellent self-healing performance	748:781	excellent self-healing performance	748:781	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	5	60	theme	%	709:709	arg1	elongation					683:692	high elongation	678:692	high elongation at break of 610% and strength of 0.11 MPa	678:734	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
35483003	5	60	theme	%	709:709	arg1	properties					798:807	recyclable properties	787:807	recyclable properties	787:807	The gel demonstrates high elongation at break of 610% and strength of 0.11 MPa, as well as excellent self-healing performance and recyclable properties.					
36961880	7	0	theme	colorimetric	1041:1052	arg1	analysis					1054:1061	colorimetric analysis	1041:1061	colorimetric analysis	1041:1061	Sub-samples were submitted for FTIR analysis and frozen for colorimetric analysis.					
36961880	3	1	theme	quarter	629:635	arg1	volume					600:605	volume	600:605	volume	600:605	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	3	1	theme	quarter	629:635	arg1	weight					589:594	1, colostral weight	576:594	weight	589:594	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	1	2	from	quarter	304:310	arg1	yield					266:270	yield	266:270	yield of colostrum from a single rear quarter	266:310	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	11	3	theme	milk	2327:2330	arg1	analysis					2341:2348	milk nutrient analysis	2327:2348	milk nutrient analysis	2327:2348	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	1	4	theme	colorimetric	419:430	arg1	methods					432:438	our developed colorimetric methods	405:438	our developed colorimetric methods	405:438	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	8	5	theme	milk	1432:1435	arg1	N					1442:1442	milk urea N	1432:1442	milk urea N	1432:1442	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	10	6	theme	N.	1876:1877	arg1	composition					1893:1903	urea N. Milk nutrient composition	1871:1903	urea N. Milk nutrient composition	1871:1903	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	9	7	theme	FTIR	1590:1593	arg1	Coefficients					1527:1538	Coefficients	1527:1538	Coefficients of determination between colorimetric methods and FTIR	1527:1593	Coefficients of determination between colorimetric methods and FTIR were determined for colostrum, d 35 milk, and d 60 milk.					
36961880	11	8	theme	complete	2194:2201	arg1	collection					2203:2212	complete collection	2194:2212	complete collection of colostrum for beef cows	2194:2239	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	3	9	theme	complete	655:662	arg1	collection					664:673	complete collection	655:673	complete collection of colostrum from post-calving, unsuckled beef heifers	655:728	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	8	10	theme	Colorimetric	1064:1075	arg1	analyses					1077:1084	Colorimetric analyses	1064:1084	Colorimetric analyses	1064:1084	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	3	11	from	collection	664:673	arg1	heifers					722:728	post-calving, unsuckled beef heifers	693:728	post-calving, unsuckled beef heifers	693:728	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	6	12	from	d	886:886	arg1	colostrum					837:845	colostrum	837:845	colostrum (n = 35)	837:854	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	12	from	d	886:886	arg1	2					834:834	2	834:834	2	834:834	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	12	from	d	886:886	arg1	milk					860:863	milk	860:863	milk at d 35 (n = 42) and d 60 (n = 38) of lactation	860:911	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	12	from	d	886:886	arg1	n					848:848	n = 35	848:853	n = 35	848:853	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	11	13	theme	nutrient	2332:2339	arg1	analysis					2341:2348	milk nutrient analysis	2327:2348	milk nutrient analysis	2327:2348	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	1	14	theme	beef	369:372	arg1	cows					374:377	beef cows	369:377	beef cows	369:377	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	8	15	theme	serum	1462:1466	arg1	N					1473:1473	serum urea N	1462:1473	serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001)	1462:1524	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	10	16	theme	nutrient	1884:1891	arg1	composition					1893:1903	urea N. Milk nutrient composition	1871:1903	urea N. Milk nutrient composition	1871:1903	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	0	17	from	composition	76:86	arg1	milk					114:117	milk	114:117	milk	114:117	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	0	17	from	composition	76:86	arg1	colostrum					100:108	beef cow colostrum	91:108	beef cow colostrum	91:108	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	8	18	dep	r	1503:1503	arg1	P					1515:1515	P < 0.001	1515:1523	P < 0.001	1515:1523	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	8	18	dep	r	1503:1503	arg1	0.72					1509:1512	0.72	1509:1512	0.72	1509:1512	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	10	19	theme	determination	2019:2031	arg1	coefficients					2003:2014	coefficients	2003:2014	coefficients of determination strengthened for all nutrients	2003:2062	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	1	20	from	collection	342:351	arg1	cows					374:377	beef cows	369:377	beef cows	369:377	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	1	21	theme	beef	221:224	arg1	colostrum					230:238	beef cow colostrum	221:238	beef cow colostrum	221:238	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	13	22	theme	cow	2480:2482	arg1	milk					2484:2487	beef cow milk	2475:2487	beef cow milk	2475:2487	Accuracy of FTIR for beef cow milk varies with component and may be affected by day of lactation.					
36961880	11	23	theme	single	2150:2155	arg1	quarter					2162:2168	a single rear quarter	2148:2168	a single rear quarter	2148:2168	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	3	24	theme	beef	717:720	arg1	heifers					722:728	post-calving, unsuckled beef heifers	693:728	post-calving, unsuckled beef heifers	693:728	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	8	25	theme	average	1248:1254	arg1	lactose					1233:1239	lactose	1233:1239	lactose (96.8% average)	1233:1255	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	8	25	theme	average	1248:1254	arg1	%					1246:1246	96.8% average	1242:1254	96.8% average	1242:1254	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	0	26	from	colostrum	100:108	arg1	determination					50:62	accurate determination	41:62	accurate determination of nutrient composition in beef cow colostrum and milk	41:117	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	3	27	theme	colostral	579:587	arg1	weight					589:594	1, colostral weight	576:594	weight	589:594	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	8	28	theme	sampling	1489:1496	arg1	r					1503:1503	r 2	1503:1505	r 2	1503:1505	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	8	28	theme	sampling	1489:1496	arg1	day					1498:1500	the same sampling day	1480:1500	the same sampling day (r 2 ≥ 0.72, P < 0.001)	1480:1524	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	10	29	theme	lactose	1662:1668	arg1	concentration					1670:1682	Colostral lactose concentration	1652:1682	Colostral lactose concentration from FTIR	1652:1692	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	11	30	contain	have	2277:2280	arg2	accuracy					2291:2298	adequate accuracy	2282:2298	adequate accuracy for beef cow colostral and milk nutrient analysis	2282:2348	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	11	30	contain	have	2277:2280	arg1	methods					2259:2265	colorimetric methods	2246:2265	colorimetric methods developed	2246:2275	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	11	31	theme	quarter	2162:2168	arg1	yield					2139:2143	colostrum yield	2129:2143	colostrum yield of a single rear quarter	2129:2168	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	0	32	theme	composition	76:86	arg1	determination					50:62	accurate determination	41:62	accurate determination of nutrient composition in beef cow colostrum and milk	41:117	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	1	33	theme	colorimetric	151:162	arg1	methods					164:170	colorimetric methods	151:170	colorimetric methods	151:170	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	10	34	theme	≥	1816:1816	arg1	P					1814:1814	P ≥ 0.14	1814:1821	P ≥ 0.14	1814:1821	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	10	34	theme	≥	1816:1816	arg1	r					1802:1802	r 2 ≤ 0.09	1802:1811	r	1802:1802	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	11	35	theme	beef	2304:2307	arg1	colostral					2313:2321	beef cow colostral	2304:2321	beef cow colostral	2304:2321	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	0	36	theme	beef	91:94	arg1	colostrum					100:108	beef cow colostrum	91:108	beef cow colostrum	91:108	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	1	37	theme	collection	342:351	arg1	representative					315:328	representative	315:328	representative	315:328	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	8	38	theme	average	1282:1288	arg1	protein					1266:1272	milk protein	1261:1272	milk protein (100.1% average)	1261:1289	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	8	38	theme	average	1282:1288	arg1	%					1280:1280	100.1% average	1275:1288	100.1% average	1275:1288	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	10	39	theme	=	2086:2086	arg1	d					2076:2076	d 60	2076:2079	d 60 (r 2 = 0.38 to 0.82, P < 0.001)	2076:2111	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	10	39	theme	=	2086:2086	arg1	r					2082:2082	r 2	2082:2084	r 2 = 0.38 to 0.82	2082:2099	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	11	40	theme	colorimetric	2246:2257	arg1	methods					2259:2265	colorimetric methods	2246:2265	colorimetric methods developed	2246:2275	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	1	41	from	cows	374:377	arg1	collection					342:351	complete collection	333:351	complete collection of colostrum in beef cows	333:377	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	1	42	theme	colostrum	356:364	arg1	collection					342:351	complete collection	333:351	complete collection of colostrum in beef cows	333:377	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	10	43	dep	=	1971:1971	arg1	0.58					1981:1984	0.58	1981:1984	0.58	1981:1984	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	6	44	from	d	868:868	arg1	colostrum					837:845	colostrum	837:845	colostrum (n = 35)	837:854	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	44	from	d	868:868	arg1	2					834:834	2	834:834	2	834:834	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	44	from	d	868:868	arg1	milk					860:863	milk	860:863	milk at d 35 (n = 42) and d 60 (n = 38) of lactation	860:911	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	44	from	d	868:868	arg1	n					848:848	n = 35	848:853	n = 35	848:853	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	10	45	theme	<	2104:2104	arg1	P					2102:2102	P < 0.001	2102:2110	P < 0.001	2102:2110	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	0	46	theme	Technical	0:8	arg1	Note					10:13	Technical Note	0:13	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.	0:118	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	1	47	theme	cow	544:546	arg1	colostrum					548:556	beef cow colostrum	539:556	beef cow colostrum	539:556	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	1	48	theme	colostrum	275:283	arg1	yield					266:270	yield	266:270	yield of colostrum from a single rear quarter	266:310	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	12	49	theme	cow	2403:2405	arg1	colostrum					2407:2415	beef cow colostrum	2398:2415	beef cow colostrum	2398:2415	Based on our analyses, nutrient composition of beef cow colostrum was not accurately analyzed by FTIR.					
36961880	10	50	dep	r	1967:1967	arg1	P					1987:1987	P < 0.001	1987:1995	P < 0.001	1987:1995	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	8	51	theme	fat	1337:1339	arg1	concentration					1341:1353	fat concentration	1337:1353	fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001)	1337:1412	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	8	52	theme	spike	1196:1200	arg1	recoveries					1202:1211	spike recoveries	1196:1211	spike recoveries	1196:1211	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	0	53	theme	accurate	41:48	arg1	determination					50:62	accurate determination	41:62	accurate determination of nutrient composition in beef cow colostrum and milk	41:117	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	1	54	theme	rear	299:302	arg1	quarter					304:310	a single rear quarter	290:310	a single rear quarter	290:310	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	10	55	from	FTIR	1689:1692	arg1	concentration					1670:1682	Colostral lactose concentration	1652:1682	Colostral lactose concentration from FTIR	1652:1692	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	13	56	theme	lactation	2541:2549	arg1	day					2534:2536	day	2534:2536	day of lactation	2534:2549	Accuracy of FTIR for beef cow milk varies with component and may be affected by day of lactation.					
36961880	10	57	theme	=	1725:1725	arg1	r					1721:1721	r 2	1721:1723	r 2 = 0.24	1721:1730	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	3	58	theme	rear	624:627	arg1	quarter					629:635	the most full rear quarter	610:635	the most full rear quarter	610:635	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	8	59	theme	method	1179:1184	arg1	accuracy					1186:1193	method accuracy	1179:1193	method accuracy	1179:1193	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	12	60	theme	nutrient	2374:2381	arg1	composition					2383:2393	nutrient composition	2374:2393	nutrient composition of beef cow colostrum	2374:2415	Based on our analyses, nutrient composition of beef cow colostrum was not accurately analyzed by FTIR.					
36961880	1	61	theme	cow	226:228	arg1	colostrum					230:238	beef cow colostrum	221:238	beef cow colostrum	221:238	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	8	62	dep	triglycerides	1114:1126	arg1	measure					1129:1135	measure	1129:1135	measure of fat	1129:1142	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	6	63	dep	multiparous	933:943	arg1	crossbred					960:968	crossbred	960:968	crossbred	960:968	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	63	dep	multiparous	933:943	arg1	fall-calving					946:957	fall-calving	946:957	fall-calving	946:957	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	10	64	dep	=	2086:2086	arg1	0.82					2096:2099	0.82	2096:2099	0.82	2096:2099	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	8	65	theme	<	1405:1405	arg1	P					1403:1403	P < 0.001	1403:1411	P < 0.001	1403:1411	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	10	66	theme	Colostral	1652:1660	arg1	concentration					1670:1682	Colostral lactose concentration	1652:1682	Colostral lactose concentration from FTIR	1652:1692	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	1	67	from	methods	432:438	arg1	data					395:398	data	395:398	data from our developed colorimetric methods	395:438	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	9	68	theme	determination	1543:1555	arg1	Coefficients					1527:1538	Coefficients	1527:1538	Coefficients of determination between colorimetric methods and FTIR	1527:1593	Coefficients of determination between colorimetric methods and FTIR were determined for colostrum, d 35 milk, and d 60 milk.					
36961880	0	69	from	milk	114:117	arg1	determination					50:62	accurate determination	41:62	accurate determination of nutrient composition in beef cow colostrum and milk	41:117	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	9	70	theme	colorimetric	1565:1576	arg1	methods					1578:1584	colorimetric methods	1565:1584	colorimetric methods	1565:1584	Coefficients of determination between colorimetric methods and FTIR were determined for colostrum, d 35 milk, and d 60 milk.					
36961880	1	71	theme	developed	409:417	arg1	methods					432:438	our developed colorimetric methods	405:438	our developed colorimetric methods	405:438	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	4	72	theme	single	789:794	arg1	quarter					796:802	single quarter	789:802	single quarter	789:802	Both volume and weight had r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields.					
36961880	10	73	theme	colorimetric	1748:1759	arg1	analysis					1761:1768	colorimetric analysis	1748:1768	colorimetric analysis	1748:1768	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	3	74	theme	colostrum	678:686	arg1	collection					664:673	complete collection	655:673	complete collection of colostrum from post-calving, unsuckled beef heifers	655:728	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	10	75	dep	r	2082:2082	arg1	P					2102:2102	P < 0.001	2102:2110	P < 0.001	2102:2110	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	10	76	theme	urea	1871:1874	arg1	composition					1893:1903	urea N. Milk nutrient composition	1871:1903	urea N. Milk nutrient composition	1871:1903	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	10	77	dep	r	1721:1721	arg1	P					1733:1733	P = 0.01	1733:1740	P = 0.01	1733:1740	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	6	78	theme	beef	970:973	arg1	cows					975:978	multiparous, fall-calving, crossbred beef cows	933:978	multiparous, fall-calving, crossbred beef cows	933:978	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	8	79	theme	urea	1437:1440	arg1	N					1442:1442	milk urea N	1432:1442	milk urea N	1432:1442	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	4	80	theme	<	772:772	arg1	P					770:770	P < 0.001	770:778	P < 0.001	770:778	Both volume and weight had r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields.					
36961880	4	80	theme	<	772:772	arg1	0.85					764:767	r 2 = 0.85	758:767	r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields	758:823	Both volume and weight had r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields.					
36961880	10	81	dep	=	1725:1725	arg1	0.24					1727:1730	0.24	1727:1730	0.24	1727:1730	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	1	82	theme	infrared	463:470	arg1	FTIR					486:489	FTIR	486:489	FTIR	486:489	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	1	82	theme	infrared	463:470	arg1	spectroscopy					472:483	infrared spectroscopy	463:483	infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk	463:565	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	1	83	theme	nutrient	194:201	arg1	concentrations					203:216	nutrient concentrations	194:216	nutrient concentrations of beef cow colostrum and milk	194:247	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	6	84	theme	=	850:850	arg1	colostrum					837:845	colostrum	837:845	colostrum (n = 35)	837:854	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	84	theme	=	850:850	arg1	n					848:848	n = 35	848:853	n = 35	848:853	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	8	85	from	day	1498:1500	arg1	N					1473:1473	serum urea N	1462:1473	serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001)	1462:1524	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	3	86	theme	post-calving	693:704	arg1	heifers					722:728	post-calving, unsuckled beef heifers	693:728	post-calving, unsuckled beef heifers	693:728	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	13	87	theme	beef	2475:2478	arg1	milk					2484:2487	beef cow milk	2475:2487	beef cow milk	2475:2487	Accuracy of FTIR for beef cow milk varies with component and may be affected by day of lactation.					
36961880	10	88	theme	Milk	1879:1882	arg1	composition					1893:1903	urea N. Milk nutrient composition	1871:1903	urea N. Milk nutrient composition	1871:1903	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	10	89	dep	associated	1709:1718	arg1	r					1721:1721	r 2	1721:1723	r 2 = 0.24	1721:1730	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	11	90	theme	colostrum	2217:2225	arg1	collection					2203:2212	complete collection	2194:2212	complete collection of colostrum for beef cows	2194:2239	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	8	91	theme	urea	1468:1471	arg1	N					1473:1473	serum urea N	1462:1473	serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001)	1462:1524	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	8	92	dep	r	1391:1391	arg1	P					1403:1403	P < 0.001	1403:1411	P < 0.001	1403:1411	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	8	92	dep	r	1391:1391	arg1	0.91					1397:1400	0.91	1397:1400	0.91	1397:1400	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	11	93	theme	rear	2157:2160	arg1	quarter					2162:2168	a single rear quarter	2148:2168	a single rear quarter	2148:2168	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	6	94	theme	=	876:876	arg1	d					868:868	d 35	868:871	d 35 (n = 42)	868:880	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	94	theme	=	876:876	arg1	n					874:874	n = 42	874:879	n = 42	874:879	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	4	95	theme	quarter	810:816	arg1	yields					818:823	4 quarter yields	808:823	4 quarter yields	808:823	Both volume and weight had r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields.					
36961880	8	96	theme	milk	1261:1264	arg1	protein					1266:1272	milk protein	1261:1272	milk protein (100.1% average)	1261:1289	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	8	96	theme	milk	1261:1264	arg1	%					1280:1280	100.1% average	1275:1288	100.1% average	1275:1288	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	9	97	theme	d	1641:1641	arg1	milk					1646:1649	d 60 milk	1641:1649	d 60 milk	1641:1649	Coefficients of determination between colorimetric methods and FTIR were determined for colostrum, d 35 milk, and d 60 milk.					
36961880	4	98	contain	had	754:756	arg2	0.85					764:767	r 2 = 0.85	758:767	r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields	758:823	Both volume and weight had r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields.					
36961880	4	98	contain	had	754:756	arg1	weight					747:752	weight	747:752	weight	747:752	Both volume and weight had r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields.					
36961880	4	98	contain	had	754:756	arg1	volume					736:741	volume	736:741	volume	736:741	Both volume and weight had r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields.					
36961880	4	98	contain	had	754:756	arg2	P					770:770	P < 0.001	770:778	P < 0.001	770:778	Both volume and weight had r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields.					
36961880	0	99	theme	nutrient	67:74	arg1	composition					76:86	nutrient composition	67:86	nutrient composition in beef cow colostrum and milk	67:117	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	8	100	theme	same	1484:1487	arg1	r					1503:1503	r 2	1503:1505	r 2	1503:1505	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	8	100	theme	same	1484:1487	arg1	day					1498:1500	the same sampling day	1480:1500	the same sampling day (r 2 ≥ 0.72, P < 0.001)	1480:1524	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	13	101	theme	FTIR	2466:2469	arg1	Accuracy					2454:2461	Accuracy	2454:2461	Accuracy of FTIR for beef cow milk	2454:2487	Accuracy of FTIR for beef cow milk varies with component and may be affected by day of lactation.					
36961880	7	102	theme	FTIR	1012:1015	arg1	analysis					1017:1024	FTIR analysis	1012:1024	FTIR analysis	1012:1024	Sub-samples were submitted for FTIR analysis and frozen for colorimetric analysis.					
36961880	10	103	theme	colostral	1844:1852	arg1	fat					1854:1856	colostral fat	1844:1856	colostral fat	1844:1856	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	3	104	dep	post-calving	693:704	arg1	unsuckled					707:715	unsuckled	707:715	unsuckled	707:715	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	3	105	from	heifers	722:728	arg1	collection					664:673	complete collection	655:673	complete collection of colostrum from post-calving, unsuckled beef heifers	655:728	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	3	105	from	heifers	722:728	arg1	colostrum					678:686	colostrum	678:686	colostrum from post-calving, unsuckled beef heifers	678:728	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	8	106	theme	fat	1140:1142	arg1	measure					1129:1135	measure	1129:1135	measure of fat	1129:1142	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	0	107	theme	cow	96:98	arg1	colostrum					100:108	beef cow colostrum	91:108	beef cow colostrum	91:108	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	1	108	theme	complete	333:340	arg1	collection					342:351	complete collection	333:351	complete collection of colostrum in beef cows	333:377	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	1	109	dep	Fourier	445:451	arg1	transform					453:461	transform	453:461	transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk	453:565	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	0	110	from	determination	50:62	arg1	milk					114:117	milk	114:117	milk	114:117	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	0	110	from	determination	50:62	arg1	colostrum					100:108	beef cow colostrum	91:108	beef cow colostrum	91:108	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	11	111	theme	cow	2309:2311	arg1	colostral					2313:2321	beef cow colostral	2304:2321	beef cow colostral	2304:2321	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	11	112	theme	beef	2231:2234	arg1	cows					2236:2239	beef cows	2231:2239	beef cows	2231:2239	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	6	113	theme	=	894:894	arg1	d					886:886	d 60	886:889	d 60 (n = 38)	886:898	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	113	theme	=	894:894	arg1	n					892:892	n = 38	892:897	n = 38	892:897	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	8	114	theme	triglyceride	1292:1303	arg1	concentration					1305:1317	triglyceride concentration	1292:1317	triglyceride concentration	1292:1317	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	1	115	theme	FTIR	530:533	arg1	accuracy					518:525	the accuracy	514:525	the accuracy of FTIR for beef cow colostrum and milk	514:565	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	12	116	theme	colostrum	2407:2415	arg1	composition					2383:2393	nutrient composition	2374:2393	nutrient composition of beef cow colostrum	2374:2415	Based on our analyses, nutrient composition of beef cow colostrum was not accurately analyzed by FTIR.					
36961880	1	117	from	colostrum	356:364	arg1	cows					374:377	beef cows	369:377	beef cows	369:377	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	10	118	dep	0.58	1981:1984	arg1	to					1978:1979	to	1978:1979	to	1978:1979	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	11	119	used	used	2177:2180	arg2	yield					2139:2143	colostrum yield	2129:2143	colostrum yield of a single rear quarter	2129:2168	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	8	120	theme	urea	1159:1162	arg1	N.					1164:1165	urea N.	1159:1165	urea N.	1159:1165	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	1	121	theme	beef	539:542	arg1	colostrum					548:556	beef cow colostrum	539:556	beef cow colostrum	539:556	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	0	122	theme	Colorimetric	16:27	arg1	methods					29:35	Colorimetric methods	16:35	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.	0:118	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	8	123	theme	<	1517:1517	arg1	P					1515:1515	P < 0.001	1515:1523	P < 0.001	1515:1523	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	10	124	theme	=	1971:1971	arg1	r					1967:1967	r 2	1967:1969	r 2 = 0.28 to 0.58	1967:1984	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	9	125	theme	d	1626:1626	arg1	milk					1631:1634	d 35 milk	1626:1634	d 35 milk	1626:1634	Coefficients of determination between colorimetric methods and FTIR were determined for colostrum, d 35 milk, and d 60 milk.					
36961880	10	126	dep	relationship	1788:1799	arg1	P					1814:1814	P ≥ 0.14	1814:1821	P ≥ 0.14	1814:1821	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	10	126	dep	relationship	1788:1799	arg1	r					1802:1802	r 2 ≤ 0.09	1802:1811	r	1802:1802	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	10	126	dep	relationship	1788:1799	arg1	0.09					1808:1811	0.09	1808:1811	0.09	1808:1811	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	10	127	theme	<	1989:1989	arg1	P					1987:1987	P < 0.001	1987:1995	P < 0.001	1987:1995	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	11	128	theme	colostrum	2129:2137	arg1	yield					2139:2143	colostrum yield	2129:2143	colostrum yield of a single rear quarter	2129:2168	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	4	129	theme	r	758:758	arg1	P					770:770	P < 0.001	770:778	P < 0.001	770:778	Both volume and weight had r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields.					
36961880	4	129	theme	r	758:758	arg1	0.85					764:767	r 2 = 0.85	758:767	r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields	758:823	Both volume and weight had r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields.					
36961880	12	130	theme	beef	2398:2401	arg1	colostrum					2407:2415	beef cow colostrum	2398:2415	beef cow colostrum	2398:2415	Based on our analyses, nutrient composition of beef cow colostrum was not accurately analyzed by FTIR.					
36961880	1	131	theme	single	292:297	arg1	quarter					304:310	a single rear quarter	290:310	a single rear quarter	290:310	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	6	132	theme	multiparous	933:943	arg1	cows					975:978	multiparous, fall-calving, crossbred beef cows	933:978	multiparous, fall-calving, crossbred beef cows	933:978	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	3	133	theme	full	619:622	arg1	quarter					629:635	the most full rear quarter	610:635	the most full rear quarter	610:635	1, colostral weight and volume of the most full rear quarter was compared with complete collection of colostrum from post-calving, unsuckled beef heifers.					
36961880	4	134	theme	=	762:762	arg1	P					770:770	P < 0.001	770:778	P < 0.001	770:778	Both volume and weight had r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields.					
36961880	4	134	theme	=	762:762	arg1	0.85					764:767	r 2 = 0.85	758:767	r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields	758:823	Both volume and weight had r 2 = 0.85 (P < 0.001) between single quarter and 4 quarter yields.					
36961880	10	135	dep	0.82	2096:2099	arg1	to					2093:2094	to	2093:2094	to	2093:2094	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	8	136	theme	Mojonnier	1373:1381	arg1	method					1383:1388	the Mojonnier method	1369:1388	the Mojonnier method (r 2 ≥ 0.91, P < 0.001)	1369:1412	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	8	136	theme	Mojonnier	1373:1381	arg1	r					1391:1391	r 2	1391:1393	r 2	1391:1393	Colorimetric analyses were developed for lactose, triglycerides (measure of fat), protein, and urea N. To validate method accuracy, spike recoveries were determined for lactose (96.8% average) and milk protein (100.1% average), triglyceride concentration was compared with fat concentration determined by the Mojonnier method (r 2 ≥ 0.91, P < 0.001), and colostral or milk urea N was compared with serum urea N from the same sampling day (r 2 ≥ 0.72, P < 0.001).					
36961880	11	137	theme	adequate	2282:2289	arg1	accuracy					2291:2298	adequate accuracy	2282:2298	adequate accuracy for beef cow colostral and milk nutrient analysis	2282:2348	In conclusion, colostrum yield of a single rear quarter can be used to indicate complete collection of colostrum for beef cows, and colorimetric methods developed have adequate accuracy for beef cow colostral and milk nutrient analysis.					
36961880	1	138	theme	colostrum	230:238	arg1	concentrations					203:216	nutrient concentrations	194:216	nutrient concentrations of beef cow colostrum and milk	194:247	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36961880	10	139	dep	associated	1920:1929	arg1	r					1967:1967	r 2	1967:1969	r 2 = 0.28 to 0.58	1967:1984	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	0	140	dep	Note	10:13	arg1	methods					29:35	Colorimetric methods	16:35	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.	0:118	Technical Note: Colorimetric methods for accurate determination of nutrient composition in beef cow colostrum and milk.					
36961880	10	141	theme	=	1735:1735	arg1	P					1733:1733	P = 0.01	1733:1740	P = 0.01	1733:1740	Colostral lactose concentration from FTIR was positively associated (r 2 = 0.24, P = 0.01) with colorimetric analysis, but there was no relationship (r 2 ≤ 0.09, P ≥ 0.14) between methods for colostral fat, protein, or urea N. Milk nutrient composition was positively associated for all nutrients measured at d 35 (r 2 = 0.28 to 0.58, P < 0.001), and coefficients of determination strengthened for all nutrients measured at d 60 (r 2 = 0.38 to 0.82, P < 0.001).					
36961880	6	142	theme	lactation	903:911	arg1	colostrum					837:845	colostrum	837:845	colostrum (n = 35)	837:854	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	142	theme	lactation	903:911	arg1	2					834:834	2	834:834	2	834:834	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	142	theme	lactation	903:911	arg1	milk					860:863	milk	860:863	milk at d 35 (n = 42) and d 60 (n = 38) of lactation	860:911	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	6	142	theme	lactation	903:911	arg1	n					848:848	n = 35	848:853	n = 35	848:853	2, colostrum (n = 35) and milk at d 35 (n = 42) and d 60 (n = 38) of lactation were collected from multiparous, fall-calving, crossbred beef cows.					
36961880	1	143	theme	milk	244:247	arg1	concentrations					203:216	nutrient concentrations	194:216	nutrient concentrations of beef cow colostrum and milk	194:247	Our objectives were to develop colorimetric methods to accurately measure nutrient concentrations of beef cow colostrum and milk, to determine if yield of colostrum from a single rear quarter is representative of complete collection of colostrum in beef cows, and to compare data from our developed colorimetric methods with Fourier transform infrared spectroscopy (FTIR) analysis to determine the accuracy of FTIR for beef cow colostrum and milk.					
36929926	1	0	from	component	128:136	arg1	milk					147:150	human milk	141:150	human milk	141:150	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	6	1	theme	oligosaccharides	1019:1034	arg1	effect					999:1004	the effect	995:1004	the effect of non-human oligosaccharides to HMOs	995:1042	Because of limited data comparing the effect of non-human oligosaccharides to HMOs, it is not known if HMOs offer an additional clinical benefit over non-human oligosaccharides.					
36929926	5	2	theme	infection	939:947	arg1	prevention					949:958	infection prevention	939:958	infection prevention	939:958	Supplementing infant formula with HMOs is safe and promotes a healthy development of the infant revealing benefits for microbiota composition and infection prevention.					
36929926	5	3	theme	microbiota	912:921	arg1	composition					923:933	microbiota composition	912:933	microbiota composition	912:933	Supplementing infant formula with HMOs is safe and promotes a healthy development of the infant revealing benefits for microbiota composition and infection prevention.					
36929926	4	4	theme	immune	735:740	arg1	system					742:747	the immune system	731:747	the immune system	731:747	HMOs fulfil a variety of physiologic functions including potential support to the immune system, brain development, and cognitive function.					
36929926	4	5	theme	functions	690:698	arg1	variety					667:673	a variety	665:673	a variety of physiologic functions including potential support to the immune system, brain development, and cognitive function	665:790	HMOs fulfil a variety of physiologic functions including potential support to the immune system, brain development, and cognitive function.					
36929926	4	5	theme	functions	690:698	arg1	functions					690:698	physiologic functions	678:698	physiologic functions including potential support to the immune system, brain development, and cognitive function	678:790	HMOs fulfil a variety of physiologic functions including potential support to the immune system, brain development, and cognitive function.					
36929926	4	5	theme	functions	690:698	arg1	support					720:726	potential support	710:726	potential support to the immune system, brain development, and cognitive function	710:790	HMOs fulfil a variety of physiologic functions including potential support to the immune system, brain development, and cognitive function.					
36929926	7	6	theme	HMO	1183:1185	arg1	composition					1187:1197	HMO composition	1183:1197	HMO composition	1183:1197	Better knowledge of the factors influencing HMO composition and their functions will help to understand their short- and long-term benefits.					
36929926	5	7	theme	infant	882:887	arg1	development					863:873	a healthy development	853:873	a healthy development of the infant revealing benefits for microbiota composition and infection prevention	853:958	Supplementing infant formula with HMOs is safe and promotes a healthy development of the infant revealing benefits for microbiota composition and infection prevention.					
36929926	1	8	theme	Human	58:62	arg1	component					128:136	the third most important solid component	97:136	the third most important solid component in human milk	97:150	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	1	8	theme	Human	58:62	arg1	HMOs					87:90	HMOs	87:90	HMOs	87:90	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	1	8	theme	Human	58:62	arg1	oligosaccharides					69:84	Human milk oligosaccharides	58:84	Human milk oligosaccharides (HMOs)	58:91	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	1	9	theme	human	141:145	arg1	milk					147:150	human milk	141:150	human milk	141:150	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	6	10	theme	limited	972:978	arg1	data					980:983	limited data	972:983	limited data comparing the effect of non-human oligosaccharides to HMOs	972:1042	Because of limited data comparing the effect of non-human oligosaccharides to HMOs, it is not known if HMOs offer an additional clinical benefit over non-human oligosaccharides.					
36929926	7	11	theme	Better	1139:1144	arg1	knowledge					1146:1154	Better knowledge	1139:1154	Better knowledge of the factors influencing HMO composition and their functions	1139:1217	Better knowledge of the factors influencing HMO composition and their functions will help to understand their short- and long-term benefits.					
36929926	1	12	theme	milk	64:67	arg1	component					128:136	the third most important solid component	97:136	the third most important solid component in human milk	97:150	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	1	12	theme	milk	64:67	arg1	HMOs					87:90	HMOs	87:90	HMOs	87:90	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	1	12	theme	milk	64:67	arg1	oligosaccharides					69:84	Human milk oligosaccharides	58:84	Human milk oligosaccharides (HMOs)	58:91	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	2	13	theme	environmental	344:356	arg1	conditions					358:367	environmental conditions	344:367	environmental conditions	344:367	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	7	14	theme	factors	1163:1169	arg1	knowledge					1146:1154	Better knowledge	1139:1154	Better knowledge of the factors influencing HMO composition and their functions	1139:1217	Better knowledge of the factors influencing HMO composition and their functions will help to understand their short- and long-term benefits.					
36929926	3	15	theme	gut	525:527	arg1	microbiome					529:538	a bifidobacterium-rich gut microbiome	502:538	a bifidobacterium-rich gut microbiome	502:538	HMOs improve the gastrointestinal barrier and also promote a bifidobacterium-rich gut microbiome, which protects against infection, strengthens the epithelial barrier, and creates immunomodulatory metabolites.					
36929926	0	16	theme	Functional	0:9	arg1	effects					11:17	Functional effects	0:17	Functional effects of human milk oligosaccharides (HMOs)	0:55	Functional effects of human milk oligosaccharides (HMOs).					
36929926	4	17	theme	potential	710:718	arg1	support					720:726	potential support	710:726	potential support to the immune system, brain development, and cognitive function	710:790	HMOs fulfil a variety of physiologic functions including potential support to the immune system, brain development, and cognitive function.					
36929926	0	18	theme	human	22:26	arg1	oligosaccharides					33:48	human milk oligosaccharides	22:48	human milk oligosaccharides (HMOs)	22:55	Functional effects of human milk oligosaccharides (HMOs).					
36929926	0	18	theme	human	22:26	arg1	HMOs					51:54	HMOs	51:54	HMOs	51:54	Functional effects of human milk oligosaccharides (HMOs).					
36929926	2	19	theme	multiple	274:281	arg1	diet					387:390	maternal diet	378:390	maternal diet	378:390	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	19	theme	multiple	274:281	arg1	conditions					358:367	environmental conditions	344:367	environmental conditions	344:367	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	19	theme	multiple	274:281	arg1	variables					283:291	multiple variables	274:291	multiple variables	274:291	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	19	theme	multiple	274:281	arg1	race					319:322	race	319:322	race	319:322	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	19	theme	multiple	274:281	arg1	mode					425:428	mode	425:428	mode of delivery	425:440	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	19	theme	multiple	274:281	arg1	weight					397:402	weight	397:402	weight	397:402	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	19	theme	multiple	274:281	arg1	season					370:375	season	370:375	season	370:375	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	19	theme	multiple	274:281	arg1	region					336:341	geographic region	325:341	geographic region	325:341	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	19	theme	multiple	274:281	arg1	status					311:316	secretor status	302:316	secretor status	302:316	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	19	theme	multiple	274:281	arg1	age					417:419	gestational age	405:419	gestational age	405:419	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	20	theme	maternal	378:385	arg1	diet					387:390	maternal diet	378:390	maternal diet	378:390	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	5	21	theme	infant	807:812	arg1	formula					814:820	infant formula	807:820	infant formula	807:820	Supplementing infant formula with HMOs is safe and promotes a healthy development of the infant revealing benefits for microbiota composition and infection prevention.					
36929926	4	22	theme	brain	750:754	arg1	development					756:766	brain development	750:766	brain development	750:766	HMOs fulfil a variety of physiologic functions including potential support to the immune system, brain development, and cognitive function.					
36929926	3	23	theme	bifidobacterium-rich	504:523	arg1	microbiome					529:538	a bifidobacterium-rich gut microbiome	502:538	a bifidobacterium-rich gut microbiome	502:538	HMOs improve the gastrointestinal barrier and also promote a bifidobacterium-rich gut microbiome, which protects against infection, strengthens the epithelial barrier, and creates immunomodulatory metabolites.					
36929926	1	24	theme	important	112:120	arg1	component					128:136	the third most important solid component	97:136	the third most important solid component in human milk	97:150	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	1	24	theme	important	112:120	arg1	oligosaccharides					69:84	Human milk oligosaccharides	58:84	Human milk oligosaccharides (HMOs)	58:91	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	0	25	theme	oligosaccharides	33:48	arg1	effects					11:17	Functional effects	0:17	Functional effects of human milk oligosaccharides (HMOs)	0:55	Functional effects of human milk oligosaccharides (HMOs).					
36929926	7	26	theme	short-	1249:1254	arg1	benefits					1270:1277	their short- and long-term benefits	1243:1277	their short- and long-term benefits	1243:1277	Better knowledge of the factors influencing HMO composition and their functions will help to understand their short- and long-term benefits.					
36929926	2	27	theme	Individual	203:212	arg1	levels					218:223	Individual HMO levels	203:223	Individual HMO levels	203:223	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	0	28	theme	milk	28:31	arg1	oligosaccharides					33:48	human milk oligosaccharides	22:48	human milk oligosaccharides (HMOs)	22:55	Functional effects of human milk oligosaccharides (HMOs).					
36929926	0	28	theme	milk	28:31	arg1	HMOs					51:54	HMOs	51:54	HMOs	51:54	Functional effects of human milk oligosaccharides (HMOs).					
36929926	1	29	with	tandem	163:168	arg1	components					191:200	other bioactive components	175:200	other bioactive components	175:200	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	3	30	theme	epithelial	591:600	arg1	barrier					602:608	the epithelial barrier	587:608	the epithelial barrier	587:608	HMOs improve the gastrointestinal barrier and also promote a bifidobacterium-rich gut microbiome, which protects against infection, strengthens the epithelial barrier, and creates immunomodulatory metabolites.					
36929926	1	31	theme	other	175:179	arg1	components					191:200	other bioactive components	175:200	other bioactive components	175:200	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	2	32	theme	gestational	405:415	arg1	age					417:419	gestational age	405:419	gestational age	405:419	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	5	33	theme	healthy	855:861	arg1	development					863:873	a healthy development	853:873	a healthy development of the infant revealing benefits for microbiota composition and infection prevention	853:958	Supplementing infant formula with HMOs is safe and promotes a healthy development of the infant revealing benefits for microbiota composition and infection prevention.					
36929926	6	34	theme	additional	1078:1087	arg1	benefit					1098:1104	an additional clinical benefit	1075:1104	an additional clinical benefit over non-human oligosaccharides	1075:1136	Because of limited data comparing the effect of non-human oligosaccharides to HMOs, it is not known if HMOs offer an additional clinical benefit over non-human oligosaccharides.					
36929926	4	35	theme	physiologic	678:688	arg1	functions					690:698	physiologic functions	678:698	physiologic functions including potential support to the immune system, brain development, and cognitive function	678:790	HMOs fulfil a variety of physiologic functions including potential support to the immune system, brain development, and cognitive function.					
36929926	4	35	theme	physiologic	678:688	arg1	support					720:726	potential support	710:726	potential support to the immune system, brain development, and cognitive function	710:790	HMOs fulfil a variety of physiologic functions including potential support to the immune system, brain development, and cognitive function.					
36929926	2	36	theme	secretor	302:309	arg1	status					311:316	secretor status	302:316	secretor status	302:316	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	1	37	theme	third	101:105	arg1	component					128:136	the third most important solid component	97:136	the third most important solid component in human milk	97:150	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	1	37	theme	third	101:105	arg1	oligosaccharides					69:84	Human milk oligosaccharides	58:84	Human milk oligosaccharides (HMOs)	58:91	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	6	38	theme	clinical	1089:1096	arg1	benefit					1098:1104	an additional clinical benefit	1075:1104	an additional clinical benefit over non-human oligosaccharides	1075:1136	Because of limited data comparing the effect of non-human oligosaccharides to HMOs, it is not known if HMOs offer an additional clinical benefit over non-human oligosaccharides.					
36929926	1	39	theme	bioactive	181:189	arg1	components					191:200	other bioactive components	175:200	other bioactive components	175:200	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	3	40	theme	gastrointestinal	460:475	arg1	barrier					477:483	the gastrointestinal barrier	456:483	the gastrointestinal barrier	456:483	HMOs improve the gastrointestinal barrier and also promote a bifidobacterium-rich gut microbiome, which protects against infection, strengthens the epithelial barrier, and creates immunomodulatory metabolites.					
36929926	6	41	theme	non-human	1111:1119	arg1	oligosaccharides					1121:1136	non-human oligosaccharides	1111:1136	non-human oligosaccharides	1111:1136	Because of limited data comparing the effect of non-human oligosaccharides to HMOs, it is not known if HMOs offer an additional clinical benefit over non-human oligosaccharides.					
36929926	6	42	theme	non-human	1009:1017	arg1	oligosaccharides					1019:1034	non-human oligosaccharides	1009:1034	non-human oligosaccharides	1009:1034	Because of limited data comparing the effect of non-human oligosaccharides to HMOs, it is not known if HMOs offer an additional clinical benefit over non-human oligosaccharides.					
36929926	3	43	theme	immunomodulatory	623:638	arg1	metabolites					640:650	immunomodulatory metabolites	623:650	immunomodulatory metabolites	623:650	HMOs improve the gastrointestinal barrier and also promote a bifidobacterium-rich gut microbiome, which protects against infection, strengthens the epithelial barrier, and creates immunomodulatory metabolites.					
36929926	4	44	theme	cognitive	773:781	arg1	function					783:790	cognitive function	773:790	cognitive function	773:790	HMOs fulfil a variety of physiologic functions including potential support to the immune system, brain development, and cognitive function.					
36929926	2	45	theme	geographic	325:334	arg1	region					336:341	geographic region	325:341	geographic region	325:341	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	46	theme	delivery	433:440	arg1	diet					387:390	maternal diet	378:390	maternal diet	378:390	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	46	theme	delivery	433:440	arg1	conditions					358:367	environmental conditions	344:367	environmental conditions	344:367	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	46	theme	delivery	433:440	arg1	race					319:322	race	319:322	race	319:322	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	46	theme	delivery	433:440	arg1	mode					425:428	mode	425:428	mode of delivery	425:440	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	46	theme	delivery	433:440	arg1	weight					397:402	weight	397:402	weight	397:402	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	46	theme	delivery	433:440	arg1	season					370:375	season	370:375	season	370:375	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	46	theme	delivery	433:440	arg1	region					336:341	geographic region	325:341	geographic region	325:341	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	46	theme	delivery	433:440	arg1	status					311:316	secretor status	302:316	secretor status	302:316	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	46	theme	delivery	433:440	arg1	age					417:419	gestational age	405:419	gestational age	405:419	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	2	47	theme	HMO	214:216	arg1	levels					218:223	Individual HMO levels	203:223	Individual HMO levels	203:223	Individual HMO levels and distribution vary greatly between mothers by multiple variables, such as secretor status, race, geographic region, environmental conditions, season, maternal diet, and weight, gestational age and mode of delivery.					
36929926	7	48	theme	long-term	1260:1268	arg1	benefits					1270:1277	their short- and long-term benefits	1243:1277	their short- and long-term benefits	1243:1277	Better knowledge of the factors influencing HMO composition and their functions will help to understand their short- and long-term benefits.					
36929926	1	49	theme	solid	122:126	arg1	component					128:136	the third most important solid component	97:136	the third most important solid component in human milk	97:150	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
36929926	1	49	theme	solid	122:126	arg1	oligosaccharides					69:84	Human milk oligosaccharides	58:84	Human milk oligosaccharides (HMOs)	58:91	Human milk oligosaccharides (HMOs) are the third most important solid component in human milk and act in tandem with other bioactive components.					
35983045	8	0	theme	cells	1195:1199	arg1	adiposity					1221:1229	bone marrow adiposity	1209:1229	bone marrow adiposity	1209:1229	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	8	0	theme	cells	1195:1199	arg1	expression					1236:1245	expression	1236:1245	expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice	1236:1331	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	8	0	theme	cells	1195:1199	arg1	expansion					1165:1173	increased expansion	1155:1173	increased expansion of mesenchymal stem cells (MSCs)	1155:1206	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	6	1	theme	bone	916:919	arg1	marrow					921:926	the bone marrow	912:926	the bone marrow of malnourished mice	912:947	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	9	2	from	osteoblasts	1473:1483	arg1	host					1505:1508	the malnourished host	1488:1508	the malnourished host	1488:1508	This suggests that inflammatory challenge promotes differentiation of BM MSCs toward the adipocyte lineage rather than toward bone-forming osteoblasts in the malnourished host.					
35983045	4	3	theme	significant	590:600	arg1	morbidity					602:610	morbidity	602:610	morbidity	602:610	Both of these infections cause significant morbidity and mortality in malnourished children.					
35983045	8	4	from	adiposity	1221:1229	arg1	mice					1328:1331	malnourished mice	1315:1331	malnourished mice	1315:1331	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	15	5	theme	dietary	2397:2403	arg1	means					2444:2448	a means	2442:2448	a means to reduce inflammation and improve bone health in malnourished children	2442:2520	Increasing dietary intake of omega-3 fatty acids may be a means to reduce inflammation and improve bone health in malnourished children.					
35983045	15	5	theme	dietary	2397:2403	arg1	intake					2405:2410	Increasing dietary intake	2386:2410	Increasing dietary intake of omega-3 fatty acids	2386:2433	Increasing dietary intake of omega-3 fatty acids may be a means to reduce inflammation and improve bone health in malnourished children.					
35983045	2	6	from	effect	195:200	arg1	composition					260:270	bone marrow composition	248:270	bone marrow composition	248:270	We investigated the effect of malnutrition and inflammatory challenge on bone marrow composition and bone health.					
35983045	2	6	from	effect	195:200	arg1	health					281:286	bone health	276:286	bone health	276:286	We investigated the effect of malnutrition and inflammatory challenge on bone marrow composition and bone health.					
35983045	10	7	theme	osteoclast	1625:1634	arg1	activity					1636:1643	enhanced osteoclast activity	1616:1643	enhanced osteoclast activity	1616:1643	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	13	8	theme	bone	2245:2248	arg1	marrow					2250:2255	the bone marrow	2241:2255	the bone marrow	2241:2255	LPS-challenged malnourished mice that received dietary fish oil showed decreased expression of inflammatory cytokines and Rankl and reduced osteoclast differentiation and activation in the bone marrow.					
35983045	6	9	theme	monocyte	887:894	arg1	accumulation					896:907	inflammatory monocyte accumulation	874:907	inflammatory monocyte accumulation in the bone marrow of malnourished mice	874:947	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	2	10	theme	bone	276:279	arg1	health					281:286	bone health	276:286	bone health	276:286	We investigated the effect of malnutrition and inflammatory challenge on bone marrow composition and bone health.					
35983045	8	11	theme	marrow	1214:1219	arg1	adiposity					1221:1229	bone marrow adiposity	1209:1229	bone marrow adiposity	1209:1229	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	12	12	theme	fish	2014:2017	arg1	acids					2007:2011	omega-3 fatty acids	1993:2011	omega-3 fatty acids (fish oil) in the nutrient-deficient diet	1993:2053	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	12	12	theme	fish	2014:2017	arg1	oil					2019:2021	fish oil	2014:2021	fish oil	2014:2021	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	6	13	theme	inflammatory	790:801	arg1	expression					812:821	increased inflammatory cytokine expression	780:821	increased inflammatory cytokine expression (Il1b, Il6, and Tnf)	780:842	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	9	14	theme	bone-forming	1460:1471	arg1	osteoblasts					1473:1483	bone-forming osteoblasts	1460:1483	bone-forming osteoblasts in the malnourished host	1460:1508	This suggests that inflammatory challenge promotes differentiation of BM MSCs toward the adipocyte lineage rather than toward bone-forming osteoblasts in the malnourished host.					
35983045	6	15	from	activation	858:867	arg1	marrow					921:926	the bone marrow	912:926	the bone marrow of malnourished mice	912:947	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	15	16	theme	omega-3	2415:2421	arg1	acids					2429:2433	omega-3 fatty acids	2415:2433	omega-3 fatty acids	2415:2433	Increasing dietary intake of omega-3 fatty acids may be a means to reduce inflammation and improve bone health in malnourished children.					
35983045	7	17	theme	LPS	1101:1103	arg1	challenge					1105:1113	LPS challenge	1101:1113	LPS challenge	1101:1113	Depletion of inflammatory monocytes in Csfr1-LysMcre-DT malnourished mice significantly reduced the inflammasome activation and IL1-ß production after LPS challenge.					
35983045	2	18	theme	challenge	235:243	arg1	effect					195:200	the effect	191:200	the effect of malnutrition and inflammatory challenge on bone marrow composition and bone health	191:286	We investigated the effect of malnutrition and inflammatory challenge on bone marrow composition and bone health.					
35983045	8	19	theme	genes	1250:1254	arg1	adiposity					1221:1229	bone marrow adiposity	1209:1229	bone marrow adiposity	1209:1229	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	8	19	theme	genes	1250:1254	arg1	expression					1236:1245	expression	1236:1245	expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice	1236:1331	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	8	19	theme	genes	1250:1254	arg1	expansion					1165:1173	increased expansion	1155:1173	increased expansion of mesenchymal stem cells (MSCs)	1155:1206	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	6	20	theme	mice	944:947	arg1	marrow					921:926	the bone marrow	912:926	the bone marrow of malnourished mice	912:947	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	3	21	theme	established	303:313	arg1	model					322:326	an established murine model	300:326	an established murine model of moderate acute malnutrition	300:357	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	10	22	theme	genes	1675:1679	arg1	increase					1576:1583	an increase	1573:1583	an increase in bone-resorbing osteoclasts	1573:1613	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	10	22	theme	genes	1675:1679	arg1	activity					1636:1643	enhanced osteoclast activity	1616:1643	enhanced osteoclast activity	1616:1643	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	10	22	theme	genes	1675:1679	arg1	upregulation					1646:1657	upregulation	1646:1657	upregulation of inflammatory genes	1646:1679	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	10	22	theme	genes	1675:1679	arg1	IL-1B					1686:1690	IL-1B	1686:1690	IL-1B involved in osteoclast differentiation and activation	1686:1744	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	5	23	theme	pronounced	686:695	arg1	changes					709:715	more pronounced bone marrow changes	681:715	more pronounced bone marrow changes that were amplified in malnourished mice	681:756	Of the 2 stimuli, LPS caused more pronounced bone marrow changes that were amplified in malnourished mice.					
35983045	13	24	theme	cytokines	2164:2172	arg1	Rankl					2178:2182	Rankl	2178:2182	Rankl	2178:2182	LPS-challenged malnourished mice that received dietary fish oil showed decreased expression of inflammatory cytokines and Rankl and reduced osteoclast differentiation and activation in the bone marrow.					
35983045	13	24	theme	cytokines	2164:2172	arg1	expression					2137:2146	decreased expression	2127:2146	decreased expression of inflammatory cytokines	2127:2172	LPS-challenged malnourished mice that received dietary fish oil showed decreased expression of inflammatory cytokines and Rankl and reduced osteoclast differentiation and activation in the bone marrow.					
35983045	5	25	theme	marrow	702:707	arg1	changes					709:715	more pronounced bone marrow changes	681:715	more pronounced bone marrow changes that were amplified in malnourished mice	681:756	Of the 2 stimuli, LPS caused more pronounced bone marrow changes that were amplified in malnourished mice.					
35983045	15	26	theme	acids	2429:2433	arg1	means					2444:2448	a means	2442:2448	a means to reduce inflammation and improve bone health in malnourished children	2442:2520	Increasing dietary intake of omega-3 fatty acids may be a means to reduce inflammation and improve bone health in malnourished children.					
35983045	15	26	theme	acids	2429:2433	arg1	intake					2405:2410	Increasing dietary intake	2386:2410	Increasing dietary intake of omega-3 fatty acids	2386:2433	Increasing dietary intake of omega-3 fatty acids may be a means to reduce inflammation and improve bone health in malnourished children.					
35983045	7	27	theme	malnourished	1006:1017	arg1	mice					1019:1022	Csfr1-LysMcre-DT malnourished mice	989:1022	Csfr1-LysMcre-DT malnourished mice	989:1022	Depletion of inflammatory monocytes in Csfr1-LysMcre-DT malnourished mice significantly reduced the inflammasome activation and IL1-ß production after LPS challenge.					
35983045	14	28	from	effect	2299:2304	arg1	marrow					2340:2345	bone marrow	2335:2345	bone marrow	2335:2345	This work demonstrates that the negative effect of inflammatory challenge on bone marrow is amplified in the malnourished host.					
35983045	3	29	theme	moderate	331:338	arg1	malnutrition					346:357	moderate acute malnutrition	331:357	moderate acute malnutrition	331:357	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	7	30	from	Depletion	950:958	arg1	mice					1019:1022	Csfr1-LysMcre-DT malnourished mice	989:1022	Csfr1-LysMcre-DT malnourished mice	989:1022	Depletion of inflammatory monocytes in Csfr1-LysMcre-DT malnourished mice significantly reduced the inflammasome activation and IL1-ß production after LPS challenge.					
35983045	0	31	theme	bone	66:69	arg1	health					71:76	bone health	66:76	bone health	66:76	Inflammatory stimuli alter bone marrow composition and compromise bone health in the malnourished host.					
35983045	13	32	theme	decreased	2127:2135	arg1	expression					2137:2146	decreased expression	2127:2146	decreased expression of inflammatory cytokines	2127:2172	LPS-challenged malnourished mice that received dietary fish oil showed decreased expression of inflammatory cytokines and Rankl and reduced osteoclast differentiation and activation in the bone marrow.					
35983045	2	33	theme	bone	248:251	arg1	composition					260:270	bone marrow composition	248:270	bone marrow composition	248:270	We investigated the effect of malnutrition and inflammatory challenge on bone marrow composition and bone health.					
35983045	4	34	from	mortality	616:624	arg1	children					642:649	malnourished children	629:649	malnourished children	629:649	Both of these infections cause significant morbidity and mortality in malnourished children.					
35983045	7	35	theme	inflammatory	963:974	arg1	monocytes					976:984	inflammatory monocytes	963:984	inflammatory monocytes	963:984	Depletion of inflammatory monocytes in Csfr1-LysMcre-DT malnourished mice significantly reduced the inflammasome activation and IL1-ß production after LPS challenge.					
35983045	1	36	theme	childhood	151:159	arg1	malnutrition					161:172	childhood malnutrition	151:172	childhood malnutrition	151:172	Inflammation has a role in the pathogenesis of childhood malnutrition.					
35983045	11	37	theme	bone	1839:1842	arg1	fragility					1844:1852	the bone fragility	1835:1852	the bone fragility associated with malnutrition	1835:1881	The resulting weakened bone formation and increased bone resorption would contribute to the bone fragility associated with malnutrition.					
35983045	0	38	theme	malnourished	85:96	arg1	host					98:101	the malnourished host	81:101	the malnourished host	81:101	Inflammatory stimuli alter bone marrow composition and compromise bone health in the malnourished host.					
35983045	13	39	theme	dietary	2103:2109	arg1	oil					2116:2118	dietary fish oil	2103:2118	dietary fish oil	2103:2118	LPS-challenged malnourished mice that received dietary fish oil showed decreased expression of inflammatory cytokines and Rankl and reduced osteoclast differentiation and activation in the bone marrow.					
35983045	3	40	theme	malnutrition	346:357	arg1	model					322:326	an established murine model	300:326	an established murine model of moderate acute malnutrition	300:357	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	14	41	theme	negative	2290:2297	arg1	effect					2299:2304	the negative effect	2286:2304	the negative effect of inflammatory challenge on bone marrow	2286:2345	This work demonstrates that the negative effect of inflammatory challenge on bone marrow is amplified in the malnourished host.					
35983045	10	42	theme	enhanced	1616:1623	arg1	activity					1636:1643	enhanced osteoclast activity	1616:1643	enhanced osteoclast activity	1616:1643	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	3	43	theme	inflammatory	387:398	arg1	challenge					400:408	acute inflammatory challenge	381:408	acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis	381:556	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	0	44	theme	Inflammatory	0:11	arg1	stimuli					13:19	Inflammatory stimuli	0:19	Inflammatory stimuli	0:19	Inflammatory stimuli alter bone marrow composition and compromise bone health in the malnourished host.					
35983045	15	45	from	health	2490:2495	arg1	children					2513:2520	malnourished children	2500:2520	malnourished children	2500:2520	Increasing dietary intake of omega-3 fatty acids may be a means to reduce inflammation and improve bone health in malnourished children.					
35983045	10	46	theme	osteoblastic	1540:1551	arg1	potential					1553:1561	this reduced osteoblastic potential	1527:1561	this reduced osteoblastic potential	1527:1561	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	9	47	theme	inflammatory	1353:1364	arg1	challenge					1366:1374	inflammatory challenge	1353:1374	inflammatory challenge	1353:1374	This suggests that inflammatory challenge promotes differentiation of BM MSCs toward the adipocyte lineage rather than toward bone-forming osteoblasts in the malnourished host.					
35983045	10	48	with	Concurrent	1511:1520	arg1	potential					1553:1561	this reduced osteoblastic potential	1527:1561	this reduced osteoblastic potential	1527:1561	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	13	49	from	activation	2227:2236	arg1	marrow					2250:2255	the bone marrow	2241:2255	the bone marrow	2241:2255	LPS-challenged malnourished mice that received dietary fish oil showed decreased expression of inflammatory cytokines and Rankl and reduced osteoclast differentiation and activation in the bone marrow.					
35983045	0	50	theme	marrow	32:37	arg1	composition					39:49	bone marrow composition	27:49	bone marrow composition	27:49	Inflammatory stimuli alter bone marrow composition and compromise bone health in the malnourished host.					
35983045	12	51	theme	fatty	1951:1955	arg1	oil					1969:1971	corn oil	1964:1971	corn oil	1964:1971	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	12	51	theme	fatty	1951:1955	arg1	acids					1957:1961	omega-6 fatty acids	1943:1961	omega-6 fatty acids (corn oil)	1943:1972	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	6	52	theme	inflammasome	845:856	arg1	activation					858:867	inflammasome activation	845:867	inflammasome activation	845:867	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	10	53	from	IL-1B	1686:1690	arg1	osteoclasts					1603:1613	bone-resorbing osteoclasts	1588:1613	bone-resorbing osteoclasts	1588:1613	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	8	54	from	expression	1236:1245	arg1	mice					1328:1331	malnourished mice	1315:1331	malnourished mice	1315:1331	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	6	55	theme	LPS	759:761	arg1	challenge					763:771	LPS challenge	759:771	LPS challenge	759:771	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	3	56	theme	sepsis	490:495	arg1	LPS					445:447	LPS	445:447	LPS	445:447	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	3	56	theme	sepsis	490:495	arg1	lipopolysaccharide					425:442	bacterial lipopolysaccharide	415:442	bacterial lipopolysaccharide (LPS)	415:448	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	3	56	theme	sepsis	490:495	arg1	surrogate					453:461	a surrogate	451:461	a surrogate of Gram-negative bacterial sepsis	451:495	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	9	57	theme	MSCs	1407:1410	arg1	differentiation					1385:1399	differentiation	1385:1399	differentiation of BM MSCs toward the adipocyte lineage rather than toward bone-forming osteoblasts in the malnourished host	1385:1508	This suggests that inflammatory challenge promotes differentiation of BM MSCs toward the adipocyte lineage rather than toward bone-forming osteoblasts in the malnourished host.					
35983045	3	58	theme	visceral	535:542	arg1	leishmaniasis					544:556	visceral leishmaniasis	535:556	visceral leishmaniasis	535:556	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	10	59	from	activity	1636:1643	arg1	osteoclasts					1603:1613	bone-resorbing osteoclasts	1588:1613	bone-resorbing osteoclasts	1588:1613	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	14	60	theme	challenge	2322:2330	arg1	effect					2299:2304	the negative effect	2286:2304	the negative effect of inflammatory challenge on bone marrow	2286:2345	This work demonstrates that the negative effect of inflammatory challenge on bone marrow is amplified in the malnourished host.					
35983045	11	61	theme	increased	1789:1797	arg1	resorption					1804:1813	increased bone resorption	1789:1813	increased bone resorption	1789:1813	The resulting weakened bone formation and increased bone resorption would contribute to the bone fragility associated with malnutrition.					
35983045	10	62	from	upregulation	1646:1657	arg1	osteoclasts					1603:1613	bone-resorbing osteoclasts	1588:1613	bone-resorbing osteoclasts	1588:1613	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	4	63	theme	malnourished	629:640	arg1	children					642:649	malnourished children	629:649	malnourished children	629:649	Both of these infections cause significant morbidity and mortality in malnourished children.					
35983045	13	64	theme	LPS-challenged	2056:2069	arg1	mice					2084:2087	LPS-challenged malnourished mice	2056:2087	LPS-challenged malnourished mice that received dietary fish oil	2056:2118	LPS-challenged malnourished mice that received dietary fish oil showed decreased expression of inflammatory cytokines and Rankl and reduced osteoclast differentiation and activation in the bone marrow.					
35983045	15	65	theme	Increasing	2386:2395	arg1	means					2444:2448	a means	2442:2448	a means to reduce inflammation and improve bone health in malnourished children	2442:2520	Increasing dietary intake of omega-3 fatty acids may be a means to reduce inflammation and improve bone health in malnourished children.					
35983045	15	65	theme	Increasing	2386:2395	arg1	intake					2405:2410	Increasing dietary intake	2386:2410	Increasing dietary intake of omega-3 fatty acids	2386:2433	Increasing dietary intake of omega-3 fatty acids may be a means to reduce inflammation and improve bone health in malnourished children.					
35983045	8	66	theme	stem	1190:1193	arg1	MSCs					1202:1205	MSCs	1202:1205	MSCs	1202:1205	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	8	66	theme	stem	1190:1193	arg1	cells					1195:1199	mesenchymal stem cells	1178:1199	mesenchymal stem cells (MSCs)	1178:1206	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	11	67	theme	weakened	1761:1768	arg1	formation					1775:1783	weakened bone formation	1761:1783	weakened bone formation	1761:1783	The resulting weakened bone formation and increased bone resorption would contribute to the bone fragility associated with malnutrition.					
35983045	13	68	from	differentiation	2207:2221	arg1	marrow					2250:2255	the bone marrow	2241:2255	the bone marrow	2241:2255	LPS-challenged malnourished mice that received dietary fish oil showed decreased expression of inflammatory cytokines and Rankl and reduced osteoclast differentiation and activation in the bone marrow.					
35983045	1	69	contain	has	117:119	arg2	role					123:126	a role	121:126	a role	121:126	Inflammation has a role in the pathogenesis of childhood malnutrition.					
35983045	1	69	contain	has	117:119	arg1	Inflammation					104:115	Inflammation	104:115	Inflammation	104:115	Inflammation has a role in the pathogenesis of childhood malnutrition.					
35983045	3	70	theme	leishmaniasis	544:556	arg1	donovani					512:519	Leishmania donovani	501:519	Leishmania donovani	501:519	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	3	70	theme	leishmaniasis	544:556	arg1	cause					526:530	the cause	522:530	the cause of visceral leishmaniasis	522:556	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	12	71	theme	nutrient-deficient	2031:2048	arg1	diet					2050:2053	the nutrient-deficient diet	2027:2053	the nutrient-deficient diet	2027:2053	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	6	72	from	accumulation	896:907	arg1	marrow					921:926	the bone marrow	912:926	the bone marrow of malnourished mice	912:947	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	1	73	theme	malnutrition	161:172	arg1	pathogenesis					135:146	the pathogenesis	131:146	the pathogenesis of childhood malnutrition	131:172	Inflammation has a role in the pathogenesis of childhood malnutrition.					
35983045	6	74	theme	inflammatory	874:885	arg1	accumulation					896:907	inflammatory monocyte accumulation	874:907	inflammatory monocyte accumulation in the bone marrow of malnourished mice	874:947	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	6	75	dep	expression	812:821	arg1	Tnf					839:841	Tnf	839:841	Tnf	839:841	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	6	75	dep	expression	812:821	arg1	Il1b					824:827	Il1b	824:827	Il1b	824:827	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	6	75	dep	expression	812:821	arg1	Il6					830:832	Il6	830:832	Il6	830:832	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	10	76	theme	inflammatory	1662:1673	arg1	genes					1675:1679	inflammatory genes	1662:1679	inflammatory genes	1662:1679	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	6	77	theme	cytokine	803:810	arg1	expression					812:821	increased inflammatory cytokine expression	780:821	increased inflammatory cytokine expression (Il1b, Il6, and Tnf)	780:842	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	8	78	theme	bone	1209:1212	arg1	adiposity					1221:1229	bone marrow adiposity	1209:1229	bone marrow adiposity	1209:1229	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	12	79	from	acids	1957:1961	arg1	rich					1935:1938	rich	1935:1938	rich	1935:1938	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	11	80	theme	bone	1799:1802	arg1	resorption					1804:1813	increased bone resorption	1789:1813	increased bone resorption	1789:1813	The resulting weakened bone formation and increased bone resorption would contribute to the bone fragility associated with malnutrition.					
35983045	6	81	theme	increased	780:788	arg1	expression					812:821	increased inflammatory cytokine expression	780:821	increased inflammatory cytokine expression (Il1b, Il6, and Tnf)	780:842	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	9	82	theme	malnourished	1492:1503	arg1	host					1505:1508	the malnourished host	1488:1508	the malnourished host	1488:1508	This suggests that inflammatory challenge promotes differentiation of BM MSCs toward the adipocyte lineage rather than toward bone-forming osteoblasts in the malnourished host.					
35983045	3	83	with	challenge	400:408	arg1	LPS					445:447	LPS	445:447	LPS	445:447	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	3	83	with	challenge	400:408	arg1	lipopolysaccharide					425:442	bacterial lipopolysaccharide	415:442	bacterial lipopolysaccharide (LPS)	415:448	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	3	83	with	challenge	400:408	arg1	surrogate					453:461	a surrogate	451:461	a surrogate of Gram-negative bacterial sepsis	451:495	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	3	83	with	challenge	400:408	arg1	donovani					512:519	Leishmania donovani	501:519	Leishmania donovani	501:519	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	3	83	with	challenge	400:408	arg1	cause					526:530	the cause	522:530	the cause of visceral leishmaniasis	522:556	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	8	84	dep	genes	1250:1254	arg1	Adipoq					1264:1269	Adipoq	1264:1269	Adipoq	1264:1269	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	8	84	dep	genes	1250:1254	arg1	Srbp1					1276:1280	Srbp1	1276:1280	Srbp1	1276:1280	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	8	84	dep	genes	1250:1254	arg1	Pparg					1257:1261	Pparg	1257:1261	Pparg	1257:1261	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	8	84	dep	genes	1250:1254	arg1	genes					1250:1254	genes	1250:1254	genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis	1250:1310	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	13	85	theme	osteoclast	2196:2205	arg1	differentiation					2207:2221	osteoclast differentiation	2196:2221	osteoclast differentiation	2196:2221	LPS-challenged malnourished mice that received dietary fish oil showed decreased expression of inflammatory cytokines and Rankl and reduced osteoclast differentiation and activation in the bone marrow.					
35983045	12	86	theme	fatty	2001:2005	arg1	acids					2007:2011	omega-3 fatty acids	1993:2011	omega-3 fatty acids (fish oil) in the nutrient-deficient diet	1993:2053	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	12	86	theme	fatty	2001:2005	arg1	oil					2019:2021	fish oil	2014:2021	fish oil	2014:2021	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	12	87	from	rich	1935:1938	arg1	oil					1969:1971	corn oil	1964:1971	corn oil	1964:1971	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	12	87	from	rich	1935:1938	arg1	acids					1957:1961	omega-6 fatty acids	1943:1961	omega-6 fatty acids (corn oil)	1943:1972	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	5	88	theme	malnourished	740:751	arg1	mice					753:756	malnourished mice	740:756	malnourished mice	740:756	Of the 2 stimuli, LPS caused more pronounced bone marrow changes that were amplified in malnourished mice.					
35983045	7	89	theme	IL1-ß	1078:1082	arg1	production					1084:1093	IL1-ß production	1078:1093	IL1-ß production	1078:1093	Depletion of inflammatory monocytes in Csfr1-LysMcre-DT malnourished mice significantly reduced the inflammasome activation and IL1-ß production after LPS challenge.					
35983045	2	90	theme	inflammatory	222:233	arg1	challenge					235:243	inflammatory challenge	222:243	inflammatory challenge	222:243	We investigated the effect of malnutrition and inflammatory challenge on bone marrow composition and bone health.					
35983045	7	91	theme	inflammasome	1050:1061	arg1	activation					1063:1072	the inflammasome activation	1046:1072	the inflammasome activation	1046:1072	Depletion of inflammatory monocytes in Csfr1-LysMcre-DT malnourished mice significantly reduced the inflammasome activation and IL1-ß production after LPS challenge.					
35983045	15	92	theme	malnourished	2500:2511	arg1	children					2513:2520	malnourished children	2500:2520	malnourished children	2500:2520	Increasing dietary intake of omega-3 fatty acids may be a means to reduce inflammation and improve bone health in malnourished children.					
35983045	6	93	theme	malnourished	931:942	arg1	mice					944:947	malnourished mice	931:947	malnourished mice	931:947	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	3	94	theme	murine	315:320	arg1	model					322:326	an established murine model	300:326	an established murine model of moderate acute malnutrition	300:357	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	5	95	theme	bone	697:700	arg1	changes					709:715	more pronounced bone marrow changes	681:715	more pronounced bone marrow changes that were amplified in malnourished mice	681:756	Of the 2 stimuli, LPS caused more pronounced bone marrow changes that were amplified in malnourished mice.					
35983045	2	96	theme	marrow	253:258	arg1	composition					260:270	bone marrow composition	248:270	bone marrow composition	248:270	We investigated the effect of malnutrition and inflammatory challenge on bone marrow composition and bone health.					
35983045	12	97	from	lipid-rich	1979:1988	arg1	acids					2007:2011	omega-3 fatty acids	1993:2011	omega-3 fatty acids (fish oil) in the nutrient-deficient diet	1993:2053	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	12	97	from	lipid-rich	1979:1988	arg1	oil					2019:2021	fish oil	2014:2021	fish oil	2014:2021	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	3	98	theme	acute	340:344	arg1	malnutrition					346:357	moderate acute malnutrition	331:357	moderate acute malnutrition	331:357	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	8	99	theme	malnourished	1315:1326	arg1	mice					1328:1331	malnourished mice	1315:1331	malnourished mice	1315:1331	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	4	100	from	morbidity	602:610	arg1	children					642:649	malnourished children	629:649	malnourished children	629:649	Both of these infections cause significant morbidity and mortality in malnourished children.					
35983045	7	101	theme	Csfr1-LysMcre-DT	989:1004	arg1	mice					1019:1022	Csfr1-LysMcre-DT malnourished mice	989:1022	Csfr1-LysMcre-DT malnourished mice	989:1022	Depletion of inflammatory monocytes in Csfr1-LysMcre-DT malnourished mice significantly reduced the inflammasome activation and IL1-ß production after LPS challenge.					
35983045	15	102	theme	bone	2485:2488	arg1	health					2490:2495	bone health	2485:2495	bone health in malnourished children	2485:2520	Increasing dietary intake of omega-3 fatty acids may be a means to reduce inflammation and improve bone health in malnourished children.					
35983045	8	103	from	expansion	1165:1173	arg1	mice					1328:1331	malnourished mice	1315:1331	malnourished mice	1315:1331	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	12	104	from	acids	2007:2011	arg1	lipid-rich					1979:1988	lipid-rich	1979:1988	lipid-rich	1979:1988	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	12	104	from	acids	2007:2011	arg1	diet					2050:2053	the nutrient-deficient diet	2027:2053	the nutrient-deficient diet	2027:2053	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	13	105	theme	inflammatory	2151:2162	arg1	cytokines					2164:2172	inflammatory cytokines	2151:2172	inflammatory cytokines	2151:2172	LPS-challenged malnourished mice that received dietary fish oil showed decreased expression of inflammatory cytokines and Rankl and reduced osteoclast differentiation and activation in the bone marrow.					
35983045	12	106	theme	omega-3	1993:1999	arg1	acids					2007:2011	omega-3 fatty acids	1993:2011	omega-3 fatty acids (fish oil) in the nutrient-deficient diet	1993:2053	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	12	106	theme	omega-3	1993:1999	arg1	oil					2019:2021	fish oil	2014:2021	fish oil	2014:2021	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	7	107	theme	monocytes	976:984	arg1	Depletion					950:958	Depletion	950:958	Depletion of inflammatory monocytes in Csfr1-LysMcre-DT malnourished mice	950:1022	Depletion of inflammatory monocytes in Csfr1-LysMcre-DT malnourished mice significantly reduced the inflammasome activation and IL1-ß production after LPS challenge.					
35983045	14	108	theme	malnourished	2367:2378	arg1	host					2380:2383	the malnourished host	2363:2383	the malnourished host	2363:2383	This work demonstrates that the negative effect of inflammatory challenge on bone marrow is amplified in the malnourished host.					
35983045	13	109	theme	fish	2111:2114	arg1	oil					2116:2118	dietary fish oil	2103:2118	dietary fish oil	2103:2118	LPS-challenged malnourished mice that received dietary fish oil showed decreased expression of inflammatory cytokines and Rankl and reduced osteoclast differentiation and activation in the bone marrow.					
35983045	12	110	theme	corn	1964:1967	arg1	oil					1969:1971	corn oil	1964:1971	corn oil	1964:1971	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	12	110	theme	corn	1964:1967	arg1	acids					1957:1961	omega-6 fatty acids	1943:1961	omega-6 fatty acids (corn oil)	1943:1972	Lastly, we evaluated the effect of replacing lipid rich in omega-6 fatty acids (corn oil) with lipid-rich in omega-3 fatty acids (fish oil) in the nutrient-deficient diet.					
35983045	10	111	theme	osteoclast	1704:1713	arg1	differentiation					1715:1729	osteoclast differentiation	1704:1729	osteoclast differentiation	1704:1729	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	0	112	theme	bone	27:30	arg1	composition					39:49	bone marrow composition	27:49	bone marrow composition	27:49	Inflammatory stimuli alter bone marrow composition and compromise bone health in the malnourished host.					
35983045	8	113	theme	inflammatory	1120:1131	arg1	challenge					1133:1141	The inflammatory challenge	1116:1141	The inflammatory challenge	1116:1141	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	3	114	theme	acute	381:385	arg1	challenge					400:408	acute inflammatory challenge	381:408	acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis	381:556	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	10	115	theme	reduced	1532:1538	arg1	potential					1553:1561	this reduced osteoblastic potential	1527:1561	this reduced osteoblastic potential	1527:1561	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	11	116	theme	bone	1770:1773	arg1	formation					1775:1783	weakened bone formation	1761:1783	weakened bone formation	1761:1783	The resulting weakened bone formation and increased bone resorption would contribute to the bone fragility associated with malnutrition.					
35983045	14	117	theme	bone	2335:2338	arg1	marrow					2340:2345	bone marrow	2335:2345	bone marrow	2335:2345	This work demonstrates that the negative effect of inflammatory challenge on bone marrow is amplified in the malnourished host.					
35983045	3	118	theme	bacterial	480:488	arg1	sepsis					490:495	Gram-negative bacterial sepsis	466:495	Gram-negative bacterial sepsis	466:495	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	10	119	from	increase	1576:1583	arg1	osteoclasts					1603:1613	bone-resorbing osteoclasts	1588:1613	bone-resorbing osteoclasts	1588:1613	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	6	120	from	expression	812:821	arg1	marrow					921:926	the bone marrow	912:926	the bone marrow of malnourished mice	912:947	LPS challenge led to increased inflammatory cytokine expression (Il1b, Il6, and Tnf), inflammasome activation, and inflammatory monocyte accumulation in the bone marrow of malnourished mice.					
35983045	11	121	theme	resulting	1751:1759	arg1	formation					1775:1783	weakened bone formation	1761:1783	weakened bone formation	1761:1783	The resulting weakened bone formation and increased bone resorption would contribute to the bone fragility associated with malnutrition.					
35983045	13	122	theme	malnourished	2071:2082	arg1	mice					2084:2087	LPS-challenged malnourished mice	2056:2087	LPS-challenged malnourished mice that received dietary fish oil	2056:2118	LPS-challenged malnourished mice that received dietary fish oil showed decreased expression of inflammatory cytokines and Rankl and reduced osteoclast differentiation and activation in the bone marrow.					
35983045	14	123	theme	inflammatory	2309:2320	arg1	challenge					2322:2330	inflammatory challenge	2309:2330	inflammatory challenge	2309:2330	This work demonstrates that the negative effect of inflammatory challenge on bone marrow is amplified in the malnourished host.					
35983045	3	124	theme	bacterial	415:423	arg1	LPS					445:447	LPS	445:447	LPS	445:447	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	3	124	theme	bacterial	415:423	arg1	lipopolysaccharide					425:442	bacterial lipopolysaccharide	415:442	bacterial lipopolysaccharide (LPS)	415:448	We studied an established murine model of moderate acute malnutrition at baseline and after acute inflammatory challenge with bacterial lipopolysaccharide (LPS), a surrogate of Gram-negative bacterial sepsis, or Leishmania donovani, the cause of visceral leishmaniasis.					
35983045	2	125	theme	malnutrition	205:216	arg1	effect					195:200	the effect	191:200	the effect of malnutrition and inflammatory challenge on bone marrow composition and bone health	191:286	We investigated the effect of malnutrition and inflammatory challenge on bone marrow composition and bone health.					
35983045	9	126	theme	BM	1404:1405	arg1	MSCs					1407:1410	BM MSCs	1404:1410	BM MSCs	1404:1410	This suggests that inflammatory challenge promotes differentiation of BM MSCs toward the adipocyte lineage rather than toward bone-forming osteoblasts in the malnourished host.					
35983045	15	127	theme	fatty	2423:2427	arg1	acids					2429:2433	omega-3 fatty acids	2415:2433	omega-3 fatty acids	2415:2433	Increasing dietary intake of omega-3 fatty acids may be a means to reduce inflammation and improve bone health in malnourished children.					
35983045	8	128	theme	mesenchymal	1178:1188	arg1	MSCs					1202:1205	MSCs	1202:1205	MSCs	1202:1205	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	8	128	theme	mesenchymal	1178:1188	arg1	cells					1195:1199	mesenchymal stem cells	1178:1199	mesenchymal stem cells (MSCs)	1178:1206	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	10	129	theme	bone-resorbing	1588:1601	arg1	osteoclasts					1603:1613	bone-resorbing osteoclasts	1588:1613	bone-resorbing osteoclasts	1588:1613	Concurrent with this reduced osteoblastic potential there was an increase in bone-resorbing osteoclasts, enhanced osteoclast activity, upregulation of inflammatory genes, and IL-1B involved in osteoclast differentiation and activation.					
35983045	8	130	theme	increased	1155:1163	arg1	expansion					1165:1173	increased expansion	1155:1173	increased expansion of mesenchymal stem cells (MSCs)	1155:1206	The inflammatory challenge also led to increased expansion of mesenchymal stem cells (MSCs), bone marrow adiposity, and expression of genes (Pparg, Adipoq, and Srbp1) associated with adipogenesis in malnourished mice.					
35983045	9	131	theme	adipocyte	1423:1431	arg1	lineage					1433:1439	the adipocyte lineage	1419:1439	the adipocyte lineage	1419:1439	This suggests that inflammatory challenge promotes differentiation of BM MSCs toward the adipocyte lineage rather than toward bone-forming osteoblasts in the malnourished host.					
35882325	2	0	from	treatment	395:403	arg1	attempt					361:367	a worthy attempt	352:367	a worthy attempt at aquaculture wastewater treatment using a self-made FO system	352:431	In this work, a worthy attempt at aquaculture wastewater treatment using a self-made FO system was conducted, confirming it was a promising approach to treating aquaculture wastewater.					
35882325	4	1	theme	cost	1015:1018	arg1	consideration					968:980	consideration	968:980	consideration of FO performance and economical cost	968:1018	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	6	2	theme	operating	1269:1277	arg1	conditions					1279:1288	optimal operating conditions	1261:1288	optimal operating conditions	1261:1288	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	9	3	theme	wastewater	2018:2027	arg1	volume					2029:2034	wastewater volume	2018:2034	wastewater volume	2018:2034	This investigation showed that employing sucrose as DS was effective for reducing wastewater volume, and it provided an alternative choice and a sustainable way for the separation of organic pollutants from water resources.					
35882325	5	4	theme	interception	1194:1205	arg1	performance					1207:1217	a great interception performance	1186:1217	a great interception performance of organic contamination	1186:1242	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination.					
35882325	6	5	dep	conditions	1279:1288	arg1	the					1248:1250	the	1248:1250	the	1248:1250	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	6	5	dep	conditions	1279:1288	arg1	basis					1252:1256	basis	1252:1256	basis	1252:1256	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	7	6	theme	time	1703:1706	arg1	component					1655:1663	the component	1651:1663	the component of the feed solution (FS) and working time	1651:1706	The results demonstrated that a layer of cake was coated on the surface of the membrane, and the main elements in the fouling cake included C, O, Na, and S, which were highly determined by the component of the feed solution (FS) and working time.					
35882325	0	7	with	osmosis	130:136	arg1	sucrose					143:149	sucrose	143:149	sucrose	143:149	Investigation on operational parameters and membrane fouling performance in treating synthetic aquaculture wastewater via forward osmosis with sucrose as draw solution.					
35882325	2	8	theme	FO	423:424	arg1	system					426:431	a self-made FO system	411:431	a self-made FO system	411:431	In this work, a worthy attempt at aquaculture wastewater treatment using a self-made FO system was conducted, confirming it was a promising approach to treating aquaculture wastewater.					
35882325	3	9	theme	running	712:718	arg1	efficiency					720:729	the running efficiency	708:729	the running efficiency	708:729	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	6	10	theme	running	1335:1341	arg1	time					1343:1346	different running time	1325:1346	different running time	1325:1346	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	5	11	theme	linear	1034:1039	arg1	relationship					1041:1052	An excellent linear relationship	1021:1052	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters	1021:1135	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination.					
35882325	3	12	theme	parameters	551:560	arg1	Optimization					523:534	Optimization	523:534	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature,	523:663	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	6	13	with	experiments	1308:1318	arg1	time					1343:1346	different running time	1325:1346	different running time	1325:1346	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	3	14	dep	solution	595:602	arg1	draw					590:593	draw	590:593	draw	590:593	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	4	15	theme	economical	1004:1013	arg1	cost					1015:1018	economical cost	1004:1018	economical cost	1004:1018	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	6	16	theme	fouling	1300:1306	arg1	experiments					1308:1318	membrane fouling experiments	1291:1318	membrane fouling experiments with different running time	1291:1346	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	3	17	theme	system	572:577	arg1	parameters					551:560	operational parameters	539:560	operational parameters	539:560	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	3	17	theme	system	572:577	arg1	temperature					652:662	DS temperature	649:662	DS temperature	649:662	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	3	17	theme	system	572:577	arg1	concentration					609:621	draw solution (DS) concentration	590:621	draw solution (DS) concentration	590:621	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	3	17	theme	system	572:577	arg1	velocity					635:642	cross-flow velocity	624:642	cross-flow velocity	624:642	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	6	18	theme	optimal	1261:1267	arg1	conditions					1279:1288	optimal operating conditions	1261:1288	optimal operating conditions	1261:1288	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	7	19	theme	feed	1672:1675	arg1	FS					1687:1688	FS	1687:1688	FS	1687:1688	The results demonstrated that a layer of cake was coated on the surface of the membrane, and the main elements in the fouling cake included C, O, Na, and S, which were highly determined by the component of the feed solution (FS) and working time.					
35882325	7	19	theme	feed	1672:1675	arg1	solution					1677:1684	the feed solution	1668:1684	the feed solution (FS)	1668:1689	The results demonstrated that a layer of cake was coated on the surface of the membrane, and the main elements in the fouling cake included C, O, Na, and S, which were highly determined by the component of the feed solution (FS) and working time.					
35882325	7	20	theme	fouling	1580:1586	arg1	cake					1588:1591	the fouling cake	1576:1591	the fouling cake	1576:1591	The results demonstrated that a layer of cake was coated on the surface of the membrane, and the main elements in the fouling cake included C, O, Na, and S, which were highly determined by the component of the feed solution (FS) and working time.					
35882325	0	21	dep	solution	159:166	arg1	draw					154:157	draw	154:157	draw	154:157	Investigation on operational parameters and membrane fouling performance in treating synthetic aquaculture wastewater via forward osmosis with sucrose as draw solution.					
35882325	2	22	theme	promising	468:476	arg1	it					459:460	it	459:460	it	459:460	In this work, a worthy attempt at aquaculture wastewater treatment using a self-made FO system was conducted, confirming it was a promising approach to treating aquaculture wastewater.					
35882325	2	22	theme	promising	468:476	arg1	approach					478:485	a promising approach	466:485	a promising approach to treating aquaculture wastewater	466:520	In this work, a worthy attempt at aquaculture wastewater treatment using a self-made FO system was conducted, confirming it was a promising approach to treating aquaculture wastewater.					
35882325	0	23	theme	operational	17:27	arg1	parameters					29:38	operational parameters	17:38	operational parameters	17:38	Investigation on operational parameters and membrane fouling performance in treating synthetic aquaculture wastewater via forward osmosis with sucrose as draw solution.					
35882325	7	24	theme	main	1559:1562	arg1	elements					1564:1571	the main elements	1555:1571	the main elements in the fouling cake	1555:1591	The results demonstrated that a layer of cake was coated on the surface of the membrane, and the main elements in the fouling cake included C, O, Na, and S, which were highly determined by the component of the feed solution (FS) and working time.					
35882325	0	25	theme	synthetic	85:93	arg1	wastewater					107:116	synthetic aquaculture wastewater	85:116	synthetic aquaculture wastewater	85:116	Investigation on operational parameters and membrane fouling performance in treating synthetic aquaculture wastewater via forward osmosis with sucrose as draw solution.					
35882325	1	26	theme	treatment	327:335	arg1	aspect					306:311	the aspect	302:311	the aspect of wastewater treatment	302:335	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	2	27	theme	aquaculture	499:509	arg1	wastewater					511:520	aquaculture wastewater	499:520	aquaculture wastewater	499:520	In this work, a worthy attempt at aquaculture wastewater treatment using a self-made FO system was conducted, confirming it was a promising approach to treating aquaculture wastewater.					
35882325	0	28	from	Investigation	0:12	arg1	parameters					29:38	operational parameters	17:38	operational parameters	17:38	Investigation on operational parameters and membrane fouling performance in treating synthetic aquaculture wastewater via forward osmosis with sucrose as draw solution.					
35882325	0	28	from	Investigation	0:12	arg1	performance					61:71	membrane fouling performance	44:71	membrane fouling performance	44:71	Investigation on operational parameters and membrane fouling performance in treating synthetic aquaculture wastewater via forward osmosis with sucrose as draw solution.					
35882325	9	29	theme	pollutants	2127:2136	arg1	separation					2105:2114	the separation	2101:2114	the separation of organic pollutants from water resources	2101:2157	This investigation showed that employing sucrose as DS was effective for reducing wastewater volume, and it provided an alternative choice and a sustainable way for the separation of organic pollutants from water resources.					
35882325	5	30	theme	oxygen	1071:1076	arg1	COD					1086:1088	COD	1086:1088	COD	1086:1088	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination.					
35882325	5	30	theme	oxygen	1071:1076	arg1	demand					1078:1083	chemical oxygen demand	1062:1083	chemical oxygen demand (COD) changing multiples and operational parameters	1062:1135	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination.					
35882325	8	31	theme	FO	1729:1730	arg1	membrane					1739:1746	the 60-h FO fouled membrane	1720:1746	the 60-h FO fouled membrane	1720:1746	Afterward, the 60-h FO fouled membrane was cleaned under the method which combined hydraulic power and chemical agents, and the water flux recovered to 12.79 Lm-2 h-1, proving a good performance for the recovery of water flux.					
35882325	4	32	with	velocity	917:924	arg1	consideration					968:980	consideration	968:980	consideration of FO performance and economical cost	968:1018	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	7	33	theme	membrane	1541:1548	arg1	surface					1526:1532	the surface	1522:1532	the surface of the membrane	1522:1548	The results demonstrated that a layer of cake was coated on the surface of the membrane, and the main elements in the fouling cake included C, O, Na, and S, which were highly determined by the component of the feed solution (FS) and working time.					
35882325	9	34	theme	water	2143:2147	arg1	resources					2149:2157	water resources	2143:2157	water resources	2143:2157	This investigation showed that employing sucrose as DS was effective for reducing wastewater volume, and it provided an alternative choice and a sustainable way for the separation of organic pollutants from water resources.					
35882325	0	35	theme	forward	122:128	arg1	osmosis					130:136	forward osmosis	122:136	forward osmosis with sucrose as draw solution	122:166	Investigation on operational parameters and membrane fouling performance in treating synthetic aquaculture wastewater via forward osmosis with sucrose as draw solution.					
35882325	8	36	theme	water	1924:1928	arg1	flux					1930:1933	water flux	1924:1933	water flux	1924:1933	Afterward, the 60-h FO fouled membrane was cleaned under the method which combined hydraulic power and chemical agents, and the water flux recovered to 12.79 Lm-2 h-1, proving a good performance for the recovery of water flux.					
35882325	4	37	theme	performance	988:998	arg1	consideration					968:980	consideration	968:980	consideration of FO performance and economical cost	968:1018	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	2	38	theme	worthy	354:359	arg1	attempt					361:367	a worthy attempt	352:367	a worthy attempt at aquaculture wastewater treatment using a self-made FO system	352:431	In this work, a worthy attempt at aquaculture wastewater treatment using a self-made FO system was conducted, confirming it was a promising approach to treating aquaculture wastewater.					
35882325	6	39	dep	morphology	1384:1393	arg1	the					1368:1370	the	1368:1370	the	1368:1370	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	2	40	theme	self-made	413:421	arg1	system					426:431	a self-made FO system	411:431	a self-made FO system	411:431	In this work, a worthy attempt at aquaculture wastewater treatment using a self-made FO system was conducted, confirming it was a promising approach to treating aquaculture wastewater.					
35882325	4	41	theme	Different	732:740	arg1	parameters					751:760	Different selected parameters	732:760	Different selected parameters	732:760	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	6	42	theme	fouled	1426:1431	arg1	membrane					1433:1440	the fouled membrane	1422:1440	the fouled membrane	1422:1440	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	4	43	with	DS	893:894	arg1	consideration					968:980	consideration	968:980	consideration of FO performance and economical cost	968:1018	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	3	44	theme	cross-flow	624:633	arg1	velocity					635:642	cross-flow velocity	624:642	cross-flow velocity	624:642	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	4	45	theme	single-factor	806:818	arg1	experiments					820:830	the single-factor experiments	802:830	the single-factor experiments	802:830	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	2	46	theme	wastewater	384:393	arg1	treatment					395:403	aquaculture wastewater treatment	372:403	aquaculture wastewater treatment using a self-made FO system	372:431	In this work, a worthy attempt at aquaculture wastewater treatment using a self-made FO system was conducted, confirming it was a promising approach to treating aquaculture wastewater.					
35882325	5	47	theme	operational	1114:1124	arg1	parameters					1126:1135	operational parameters	1114:1135	operational parameters	1114:1135	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination.					
35882325	0	48	theme	fouling	53:59	arg1	performance					61:71	membrane fouling performance	44:71	membrane fouling performance	44:71	Investigation on operational parameters and membrane fouling performance in treating synthetic aquaculture wastewater via forward osmosis with sucrose as draw solution.					
35882325	4	49	with	temperature	942:952	arg1	consideration					968:980	consideration	968:980	consideration of FO performance and economical cost	968:1018	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	3	50	theme	solution	595:602	arg1	concentration					609:621	draw solution (DS) concentration	590:621	draw solution (DS) concentration	590:621	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	3	51	theme	DS	649:650	arg1	temperature					652:662	DS temperature	649:662	DS temperature	649:662	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	4	52	theme	water	784:788	arg1	flux					790:793	the water flux	780:793	the water flux during the single-factor experiments	780:830	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	5	53	theme	organic	1222:1228	arg1	contamination					1230:1242	organic contamination	1222:1242	organic contamination	1222:1242	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination.					
35882325	6	54	theme	microscopic	1372:1382	arg1	morphology					1384:1393	microscopic morphology	1372:1393	microscopic morphology	1372:1393	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	1	55	theme	concentration	241:253	arg1	gradient					255:262	a natural concentration gradient	231:262	a natural concentration gradient	231:262	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	1	56	from	strategy	290:297	arg1	aspect					306:311	the aspect	302:311	the aspect of wastewater treatment	302:335	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	5	57	theme	great	1188:1192	arg1	performance					1207:1217	a great interception performance	1186:1217	a great interception performance of organic contamination	1186:1242	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination.					
35882325	4	58	theme	optimal	869:875	arg1	DS					893:894	DS	893:894	DS of 1.5 M	893:903	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	4	58	theme	optimal	869:875	arg1	conditions					877:886	the optimal conditions	865:886	the optimal conditions	865:886	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	7	59	theme	working	1695:1701	arg1	time					1703:1706	working time	1695:1706	working time	1695:1706	The results demonstrated that a layer of cake was coated on the surface of the membrane, and the main elements in the fouling cake included C, O, Na, and S, which were highly determined by the component of the feed solution (FS) and working time.					
35882325	8	60	theme	hydraulic	1792:1800	arg1	power					1802:1806	hydraulic power	1792:1806	hydraulic power	1792:1806	Afterward, the 60-h FO fouled membrane was cleaned under the method which combined hydraulic power and chemical agents, and the water flux recovered to 12.79 Lm-2 h-1, proving a good performance for the recovery of water flux.					
35882325	6	61	theme	element	1399:1405	arg1	composition					1407:1417	element composition	1399:1417	element composition	1399:1417	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	8	62	theme	Lm-2	1867:1870	arg1	h-1					1872:1874	12.79 Lm-2 h-1	1861:1874	12.79 Lm-2 h-1	1861:1874	Afterward, the 60-h FO fouled membrane was cleaned under the method which combined hydraulic power and chemical agents, and the water flux recovered to 12.79 Lm-2 h-1, proving a good performance for the recovery of water flux.					
35882325	3	63	theme	operational	539:549	arg1	parameters					551:560	operational parameters	539:560	operational parameters	539:560	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	3	63	theme	operational	539:549	arg1	temperature					652:662	DS temperature	649:662	DS temperature	649:662	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	3	63	theme	operational	539:549	arg1	concentration					609:621	draw solution (DS) concentration	590:621	draw solution (DS) concentration	590:621	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	3	63	theme	operational	539:549	arg1	velocity					635:642	cross-flow velocity	624:642	cross-flow velocity	624:642	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	4	64	theme	M	903:903	arg1	temperature					942:952	temperature	942:952	temperature of 32 °C	942:961	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	4	64	theme	M	903:903	arg1	conditions					877:886	the optimal conditions	865:886	the optimal conditions	865:886	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	4	64	theme	M	903:903	arg1	DS					893:894	DS	893:894	DS of 1.5 M	893:903	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	4	64	theme	M	903:903	arg1	velocity					917:924	cross-flow velocity	906:924	cross-flow velocity of 15 cm/s	906:935	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	9	65	theme	alternative	2056:2066	arg1	choice					2068:2073	an alternative choice	2053:2073	an alternative choice	2053:2073	This investigation showed that employing sucrose as DS was effective for reducing wastewater volume, and it provided an alternative choice and a sustainable way for the separation of organic pollutants from water resources.					
35882325	6	66	theme	different	1325:1333	arg1	time					1343:1346	different running time	1325:1346	different running time	1325:1346	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	1	67	theme	separation	202:211	arg1	osmosis					177:183	Forward osmosis	169:183	Forward osmosis (FO)	169:188	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	1	67	theme	separation	202:211	arg1	process					213:219	a membrane separation process	191:219	a membrane separation process driven by a natural concentration gradient	191:262	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	4	68	theme	cross-flow	906:915	arg1	velocity					917:924	cross-flow velocity	906:924	cross-flow velocity of 15 cm/s	906:935	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	8	69	theme	water	1837:1841	arg1	flux					1843:1846	the water flux	1833:1846	the water flux	1833:1846	Afterward, the 60-h FO fouled membrane was cleaned under the method which combined hydraulic power and chemical agents, and the water flux recovered to 12.79 Lm-2 h-1, proving a good performance for the recovery of water flux.					
35882325	5	70	theme	chemical	1062:1069	arg1	COD					1086:1088	COD	1086:1088	COD	1086:1088	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination.					
35882325	5	70	theme	chemical	1062:1069	arg1	demand					1078:1083	chemical oxygen demand	1062:1083	chemical oxygen demand (COD) changing multiples and operational parameters	1062:1135	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination.					
35882325	3	71	theme	FO	569:570	arg1	system					572:577	the FO system	565:577	the FO system	565:577	Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency.					
35882325	9	72	theme	sustainable	2081:2091	arg1	way					2093:2095	a sustainable way	2079:2095	a sustainable way for the separation of organic pollutants from water resources	2079:2157	This investigation showed that employing sucrose as DS was effective for reducing wastewater volume, and it provided an alternative choice and a sustainable way for the separation of organic pollutants from water resources.					
35882325	6	73	theme	membrane	1291:1298	arg1	experiments					1308:1318	membrane fouling experiments	1291:1318	membrane fouling experiments with different running time	1291:1346	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	7	74	theme	solution	1677:1684	arg1	component					1655:1663	the component	1651:1663	the component of the feed solution (FS) and working time	1651:1706	The results demonstrated that a layer of cake was coated on the surface of the membrane, and the main elements in the fouling cake included C, O, Na, and S, which were highly determined by the component of the feed solution (FS) and working time.					
35882325	8	75	theme	12.79	1861:1865	arg1	Lm-2					1867:1870	Lm-2	1867:1870	Lm-2	1867:1870	Afterward, the 60-h FO fouled membrane was cleaned under the method which combined hydraulic power and chemical agents, and the water flux recovered to 12.79 Lm-2 h-1, proving a good performance for the recovery of water flux.					
35882325	5	76	theme	excellent	1024:1032	arg1	relationship					1041:1052	An excellent linear relationship	1021:1052	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters	1021:1135	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination.					
35882325	0	77	theme	aquaculture	95:105	arg1	wastewater					107:116	synthetic aquaculture wastewater	85:116	synthetic aquaculture wastewater	85:116	Investigation on operational parameters and membrane fouling performance in treating synthetic aquaculture wastewater via forward osmosis with sucrose as draw solution.					
35882325	1	78	theme	wastewater	316:325	arg1	treatment					327:335	wastewater treatment	316:335	wastewater treatment	316:335	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	9	79	from	resources	2149:2157	arg1	separation					2105:2114	the separation	2101:2114	the separation of organic pollutants from water resources	2101:2157	This investigation showed that employing sucrose as DS was effective for reducing wastewater volume, and it provided an alternative choice and a sustainable way for the separation of organic pollutants from water resources.					
35882325	1	80	theme	Forward	169:175	arg1	process					213:219	a membrane separation process	191:219	a membrane separation process driven by a natural concentration gradient	191:262	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	1	80	theme	Forward	169:175	arg1	FO					186:187	FO	186:187	FO	186:187	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	1	80	theme	Forward	169:175	arg1	osmosis					177:183	Forward osmosis	169:183	Forward osmosis (FO)	169:188	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	8	81	theme	chemical	1812:1819	arg1	agents					1821:1826	chemical agents	1812:1826	chemical agents	1812:1826	Afterward, the 60-h FO fouled membrane was cleaned under the method which combined hydraulic power and chemical agents, and the water flux recovered to 12.79 Lm-2 h-1, proving a good performance for the recovery of water flux.					
35882325	9	82	theme	organic	2119:2125	arg1	pollutants					2127:2136	organic pollutants	2119:2136	organic pollutants	2119:2136	This investigation showed that employing sucrose as DS was effective for reducing wastewater volume, and it provided an alternative choice and a sustainable way for the separation of organic pollutants from water resources.					
35882325	4	83	theme	selected	742:749	arg1	parameters					751:760	Different selected parameters	732:760	Different selected parameters	732:760	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	8	84	theme	good	1887:1890	arg1	performance					1892:1902	a good performance	1885:1902	a good performance	1885:1902	Afterward, the 60-h FO fouled membrane was cleaned under the method which combined hydraulic power and chemical agents, and the water flux recovered to 12.79 Lm-2 h-1, proving a good performance for the recovery of water flux.					
35882325	1	85	theme	membrane	193:200	arg1	osmosis					177:183	Forward osmosis	169:183	Forward osmosis (FO)	169:188	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	1	85	theme	membrane	193:200	arg1	process					213:219	a membrane separation process	191:219	a membrane separation process driven by a natural concentration gradient	191:262	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	4	86	theme	cm/s	932:935	arg1	temperature					942:952	temperature	942:952	temperature of 32 °C	942:961	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	4	86	theme	cm/s	932:935	arg1	conditions					877:886	the optimal conditions	865:886	the optimal conditions	865:886	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	4	86	theme	cm/s	932:935	arg1	DS					893:894	DS	893:894	DS of 1.5 M	893:903	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	4	86	theme	cm/s	932:935	arg1	velocity					917:924	cross-flow velocity	906:924	cross-flow velocity of 15 cm/s	906:935	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	8	87	theme	flux	1930:1933	arg1	recovery					1912:1919	the recovery	1908:1919	the recovery of water flux	1908:1933	Afterward, the 60-h FO fouled membrane was cleaned under the method which combined hydraulic power and chemical agents, and the water flux recovered to 12.79 Lm-2 h-1, proving a good performance for the recovery of water flux.					
35882325	8	88	theme	fouled	1732:1737	arg1	membrane					1739:1746	the 60-h FO fouled membrane	1720:1746	the 60-h FO fouled membrane	1720:1746	Afterward, the 60-h FO fouled membrane was cleaned under the method which combined hydraulic power and chemical agents, and the water flux recovered to 12.79 Lm-2 h-1, proving a good performance for the recovery of water flux.					
35882325	4	89	theme	FO	985:986	arg1	performance					988:998	FO performance	985:998	FO performance	985:998	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	6	90	theme	membrane	1433:1440	arg1	morphology					1384:1393	microscopic morphology	1372:1393	microscopic morphology	1372:1393	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	6	90	theme	membrane	1433:1440	arg1	composition					1407:1417	element composition	1399:1417	element composition	1399:1417	On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed.					
35882325	5	91	theme	experimental	1155:1166	arg1	results					1168:1174	experimental results	1155:1174	experimental results	1155:1174	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination.					
35882325	8	92	theme	60-h	1724:1727	arg1	membrane					1739:1746	the 60-h FO fouled membrane	1720:1746	the 60-h FO fouled membrane	1720:1746	Afterward, the 60-h FO fouled membrane was cleaned under the method which combined hydraulic power and chemical agents, and the water flux recovered to 12.79 Lm-2 h-1, proving a good performance for the recovery of water flux.					
35882325	0	93	theme	membrane	44:51	arg1	performance					61:71	membrane fouling performance	44:71	membrane fouling performance	44:71	Investigation on operational parameters and membrane fouling performance in treating synthetic aquaculture wastewater via forward osmosis with sucrose as draw solution.					
35882325	1	94	theme	potential	280:288	arg1	strategy					290:297	a potential strategy	278:297	a potential strategy in the aspect of wastewater treatment	278:335	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	4	95	theme	°C	960:961	arg1	temperature					942:952	temperature	942:952	temperature of 32 °C	942:961	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	4	95	theme	°C	960:961	arg1	conditions					877:886	the optimal conditions	865:886	the optimal conditions	865:886	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	4	95	theme	°C	960:961	arg1	DS					893:894	DS	893:894	DS of 1.5 M	893:903	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	4	95	theme	°C	960:961	arg1	velocity					917:924	cross-flow velocity	906:924	cross-flow velocity of 15 cm/s	906:935	Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost.					
35882325	2	96	theme	aquaculture	372:382	arg1	treatment					395:403	aquaculture wastewater treatment	372:403	aquaculture wastewater treatment using a self-made FO system	372:431	In this work, a worthy attempt at aquaculture wastewater treatment using a self-made FO system was conducted, confirming it was a promising approach to treating aquaculture wastewater.					
35882325	7	97	from	elements	1564:1571	arg1	cake					1588:1591	the fouling cake	1576:1591	the fouling cake	1576:1591	The results demonstrated that a layer of cake was coated on the surface of the membrane, and the main elements in the fouling cake included C, O, Na, and S, which were highly determined by the component of the feed solution (FS) and working time.					
35882325	7	98	theme	cake	1503:1506	arg1	layer					1494:1498	a layer	1492:1498	a layer of cake	1492:1506	The results demonstrated that a layer of cake was coated on the surface of the membrane, and the main elements in the fouling cake included C, O, Na, and S, which were highly determined by the component of the feed solution (FS) and working time.					
35882325	1	99	theme	natural	233:239	arg1	gradient					255:262	a natural concentration gradient	231:262	a natural concentration gradient	231:262	Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment.					
35882325	5	100	theme	contamination	1230:1242	arg1	performance					1207:1217	a great interception performance	1186:1217	a great interception performance of organic contamination	1186:1242	An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination.					
36162531	7	0	theme	granulation	1604:1614	arg1	formation					1623:1631	granulation tissue formation	1604:1631	granulation tissue formation	1604:1631	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	5	1	dep	in	1119:1120	arg1	vivo					1122:1125	vivo	1122:1125	vivo	1122:1125	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	3	2	theme	proton	719:724	arg1	resonance					743:751	proton nuclear magnetic resonance	719:751	proton nuclear magnetic resonance spectroscopy	719:764	The synthesis of COS-SA and HAMA was confirmed by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and polyphenol assay.					
36162531	7	3	theme	re-epithelization	1585:1601	arg1	promotion					1534:1542	the significant promotion	1518:1542	the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application	1518:1790	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	0	4	link	cross-linked	21:32	arg1	poly					34:37	Multifunctional dual cross-linked poly	0:37	Multifunctional dual cross-linked poly (vinyl alcohol)	0:53	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	0	4	link	cross-linked	21:32	arg1	alcohol					46:52	vinyl alcohol	40:52	vinyl alcohol	40:52	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	6	5	theme	uniform	1261:1267	arg1	architecture					1276:1287	a uniform porous architecture	1259:1287	a uniform porous architecture	1259:1287	The results showed that the fabricated composite hydrogel had a uniform porous architecture, excellent fluid absorbability, and appropriate mechanical stability.					
36162531	3	6	theme	ultraviolet	767:777	arg1	spectroscopy					779:790	ultraviolet spectroscopy	767:790	ultraviolet spectroscopy	767:790	The synthesis of COS-SA and HAMA was confirmed by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and polyphenol assay.					
36162531	1	7	theme	great	321:325	arg1	interest					327:334	great interest	321:334	great interest in wound healing application	321:363	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	7	8	theme	closure	1576:1582	arg1	promotion					1534:1542	the significant promotion	1518:1542	the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application	1518:1790	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	4	9	theme	composite	889:897	arg1	hydrogel					899:906	duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel	842:906	duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application	842:1021	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	7	10	theme	significant	1522:1532	arg1	promotion					1534:1542	the significant promotion	1518:1542	the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application	1518:1790	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	7	11	theme	full-thickness	1555:1568	arg1	closure					1576:1582	in vivo full-thickness wound closure	1547:1582	in vivo full-thickness wound closure	1547:1582	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	6	12	theme	composite	1236:1244	arg1	hydrogel					1246:1253	the fabricated composite hydrogel	1221:1253	the fabricated composite hydrogel	1221:1253	The results showed that the fabricated composite hydrogel had a uniform porous architecture, excellent fluid absorbability, and appropriate mechanical stability.					
36162531	3	13	theme	polyphenol	797:806	arg1	assay					808:812	polyphenol assay	797:812	polyphenol assay	797:812	The synthesis of COS-SA and HAMA was confirmed by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and polyphenol assay.					
36162531	6	14	theme	appropriate	1325:1335	arg1	stability					1348:1356	appropriate mechanical stability	1325:1356	appropriate mechanical stability	1325:1356	The results showed that the fabricated composite hydrogel had a uniform porous architecture, excellent fluid absorbability, and appropriate mechanical stability.					
36162531	4	15	theme	full-thickness	982:995	arg1	healing					1003:1009	full-thickness wound healing	982:1009	full-thickness wound healing application	982:1021	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	5	16	theme	wound	1127:1131	arg1	healing					1133:1139	in vivo wound healing	1119:1139	in vivo wound healing	1119:1139	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	1	17	theme	appropriate	255:265	arg1	properties					304:313	appropriate mechanical, biological, and physical properties	255:313	appropriate mechanical, biological, and physical properties	255:313	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	4	18	theme	healing	1003:1009	arg1	application					1011:1021	full-thickness wound healing application	982:1021	full-thickness wound healing application	982:1021	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	7	19	theme	wound	1766:1770	arg1	healing					1772:1778	chronic wound healing	1758:1778	chronic wound healing application	1758:1790	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	7	20	dep	incorporated	1682:1693	arg1	COSs-SA					1674:1680	COSs-SA	1674:1680	COSs-SA	1674:1680	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	1	21	theme	dressing	149:156	arg1	hydrogel					158:165	Wound dressing hydrogel	143:165	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties	143:313	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	6	22	theme	excellent	1290:1298	arg1	absorbability					1306:1318	excellent fluid absorbability	1290:1318	excellent fluid absorbability	1290:1318	The results showed that the fabricated composite hydrogel had a uniform porous architecture, excellent fluid absorbability, and appropriate mechanical stability.					
36162531	5	23	theme	in	1119:1120	arg1	healing					1133:1139	in vivo wound healing	1119:1139	in vivo wound healing	1119:1139	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	7	24	dep	in	1547:1548	arg1	vivo					1550:1553	vivo	1550:1553	vivo	1550:1553	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	7	25	theme	significant	1732:1742	arg1	potential					1744:1752	significant potential	1732:1752	significant potential for chronic wound healing application	1732:1790	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	2	26	theme	grafting	517:524	arg1	polymerization					526:539	H2O2-induced grafting polymerization	504:539	H2O2-induced grafting polymerization	504:539	In the present study, chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA) was synthesized using H2O2-induced grafting polymerization, and photo cross-linkable hyaluronic acid was synthesized using methacrilation (HAMA).					
36162531	0	27	theme	acid/chitooligosaccharide-sinapic	79:111	arg1	hydrogel					133:140	hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel	68:140	hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel	68:140	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	7	28	theme	hydrogel	1704:1711	arg1	dressing					1719:1726	COSs-SA incorporated PVA/HAMA hydrogel wound dressing	1674:1726	COSs-SA incorporated PVA/HAMA hydrogel wound dressing	1674:1726	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	2	29	theme	photo	546:550	arg1	acid					578:581	photo cross-linkable hyaluronic acid	546:581	photo cross-linkable hyaluronic acid	546:581	In the present study, chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA) was synthesized using H2O2-induced grafting polymerization, and photo cross-linkable hyaluronic acid was synthesized using methacrilation (HAMA).					
36162531	0	30	theme	wound	118:122	arg1	hydrogel					133:140	hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel	68:140	hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel	68:140	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	5	31	theme	hydrogels	1155:1163	arg1	properties					1141:1150	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties	1024:1150	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels	1024:1163	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	7	32	theme	incorporated	1682:1693	arg1	dressing					1719:1726	COSs-SA incorporated PVA/HAMA hydrogel wound dressing	1674:1726	COSs-SA incorporated PVA/HAMA hydrogel wound dressing	1674:1726	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	0	33	theme	vinyl	40:44	arg1	poly					34:37	Multifunctional dual cross-linked poly	0:37	Multifunctional dual cross-linked poly (vinyl alcohol)	0:53	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	0	33	theme	vinyl	40:44	arg1	alcohol					46:52	vinyl alcohol	40:52	vinyl alcohol	40:52	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	2	34	theme	chitooligosaccharides	416:436	arg1	COS-SA					474:479	COS-SA	474:479	COS-SA	474:479	In the present study, chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA) was synthesized using H2O2-induced grafting polymerization, and photo cross-linkable hyaluronic acid was synthesized using methacrilation (HAMA).					
36162531	2	34	theme	chitooligosaccharides	416:436	arg1	conjugate					463:471	chitooligosaccharides (COS)/ sinapic acid (SA) conjugate	416:471	chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA)	416:480	In the present study, chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA) was synthesized using H2O2-induced grafting polymerization, and photo cross-linkable hyaluronic acid was synthesized using methacrilation (HAMA).					
36162531	0	35	theme	Multifunctional	0:14	arg1	poly					34:37	Multifunctional dual cross-linked poly	0:37	Multifunctional dual cross-linked poly (vinyl alcohol)	0:53	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	0	35	theme	Multifunctional	0:14	arg1	alcohol					46:52	vinyl alcohol	40:52	vinyl alcohol	40:52	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	0	36	theme	cross-linked	21:32	arg1	poly					34:37	Multifunctional dual cross-linked poly	0:37	Multifunctional dual cross-linked poly (vinyl alcohol)	0:53	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	0	36	theme	cross-linked	21:32	arg1	alcohol					46:52	vinyl alcohol	40:52	vinyl alcohol	40:52	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	1	37	theme	wound	339:343	arg1	healing					345:351	wound healing	339:351	wound healing application	339:363	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	5	38	theme	chemical	1028:1035	arg1	antimicrobial					1073:1085	chemical, physical, mechanical, antioxidant, antimicrobial	1028:1085	chemical, physical, mechanical, antioxidant, antimicrobial	1028:1085	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	4	39	link	cross-linked	847:858	arg1	PVA					879:881	PVA	879:881	PVA	879:881	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	4	39	link	cross-linked	847:858	arg1	alcohol					870:876	cross-linked polyvinyl alcohol	847:876	duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application	842:1021	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	7	40	theme	collagen	1638:1645	arg1	deposition					1647:1656	collagen deposition	1638:1656	collagen deposition	1638:1656	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	5	41	theme	in	1088:1089	arg1	biocompatibility					1097:1112	in vitro biocompatibility	1088:1112	in vitro biocompatibility	1088:1112	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	5	42	theme	biocompatibility	1097:1112	arg1	properties					1141:1150	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties	1024:1150	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels	1024:1163	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	7	43	theme	COSs-SA	1379:1385	arg1	conjugate					1387:1395	COSs-SA conjugate	1379:1395	COSs-SA conjugate	1379:1395	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	2	44	dep	chitooligosaccharides	416:436	arg1	SA					459:460	SA	459:460	SA	459:460	In the present study, chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA) was synthesized using H2O2-induced grafting polymerization, and photo cross-linkable hyaluronic acid was synthesized using methacrilation (HAMA).					
36162531	2	44	dep	chitooligosaccharides	416:436	arg1	acid					453:456	sinapic acid	445:456	chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA)	416:480	In the present study, chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA) was synthesized using H2O2-induced grafting polymerization, and photo cross-linkable hyaluronic acid was synthesized using methacrilation (HAMA).					
36162531	1	45	theme	biological	279:288	arg1	properties					304:313	appropriate mechanical, biological, and physical properties	255:313	appropriate mechanical, biological, and physical properties	255:313	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	7	46	theme	tissue	1616:1621	arg1	formation					1623:1631	granulation tissue formation	1604:1631	granulation tissue formation	1604:1631	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	6	47	contain	had	1255:1257	arg2	absorbability					1306:1318	excellent fluid absorbability	1290:1318	excellent fluid absorbability	1290:1318	The results showed that the fabricated composite hydrogel had a uniform porous architecture, excellent fluid absorbability, and appropriate mechanical stability.					
36162531	6	47	contain	had	1255:1257	arg2	architecture					1276:1287	a uniform porous architecture	1259:1287	a uniform porous architecture	1259:1287	The results showed that the fabricated composite hydrogel had a uniform porous architecture, excellent fluid absorbability, and appropriate mechanical stability.					
36162531	6	47	contain	had	1255:1257	arg1	hydrogel					1246:1253	the fabricated composite hydrogel	1221:1253	the fabricated composite hydrogel	1221:1253	The results showed that the fabricated composite hydrogel had a uniform porous architecture, excellent fluid absorbability, and appropriate mechanical stability.					
36162531	6	47	contain	had	1255:1257	arg2	stability					1348:1356	appropriate mechanical stability	1325:1356	appropriate mechanical stability	1325:1356	The results showed that the fabricated composite hydrogel had a uniform porous architecture, excellent fluid absorbability, and appropriate mechanical stability.					
36162531	3	48	theme	infrared	696:703	arg1	spectroscopy					705:716	Fourier-transform infrared spectroscopy	678:716	Fourier-transform infrared spectroscopy	678:716	The synthesis of COS-SA and HAMA was confirmed by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and polyphenol assay.					
36162531	4	49	theme	polyvinyl	860:868	arg1	PVA					879:881	PVA	879:881	PVA	879:881	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	4	49	theme	polyvinyl	860:868	arg1	alcohol					870:876	cross-linked polyvinyl alcohol	847:876	duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application	842:1021	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	7	50	contain	has	1728:1730	arg1	dressing					1719:1726	COSs-SA incorporated PVA/HAMA hydrogel wound dressing	1674:1726	COSs-SA incorporated PVA/HAMA hydrogel wound dressing	1674:1726	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	7	50	contain	has	1728:1730	arg2	potential					1744:1752	significant potential	1732:1752	significant potential for chronic wound healing application	1732:1790	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	3	51	theme	resonance	743:751	arg1	spectroscopy					753:764	proton nuclear magnetic resonance spectroscopy	719:764	proton nuclear magnetic resonance spectroscopy	719:764	The synthesis of COS-SA and HAMA was confirmed by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and polyphenol assay.					
36162531	6	52	theme	mechanical	1337:1346	arg1	stability					1348:1356	appropriate mechanical stability	1325:1356	appropriate mechanical stability	1325:1356	The results showed that the fabricated composite hydrogel had a uniform porous architecture, excellent fluid absorbability, and appropriate mechanical stability.					
36162531	3	53	theme	nuclear	726:732	arg1	resonance					743:751	proton nuclear magnetic resonance	719:751	proton nuclear magnetic resonance spectroscopy	719:764	The synthesis of COS-SA and HAMA was confirmed by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and polyphenol assay.					
36162531	7	54	theme	wound	1570:1574	arg1	closure					1576:1582	in vivo full-thickness wound closure	1547:1582	in vivo full-thickness wound closure	1547:1582	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	7	55	theme	in	1547:1548	arg1	closure					1576:1582	in vivo full-thickness wound closure	1547:1582	in vivo full-thickness wound closure	1547:1582	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	1	56	theme	antioxidant	210:220	arg1	properties					240:249	antioxidant and antimicrobial properties	210:249	antioxidant and antimicrobial properties	210:249	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	4	57	theme	/HAMA	883:887	arg1	hydrogel					899:906	duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel	842:906	duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application	842:1021	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	1	58	from	interest	327:334	arg1	application					353:363	wound healing application	339:363	wound healing application	339:363	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	1	59	theme	antimicrobial	226:238	arg1	properties					240:249	antioxidant and antimicrobial properties	210:249	antioxidant and antimicrobial properties	210:249	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	6	60	theme	fabricated	1225:1234	arg1	hydrogel					1246:1253	the fabricated composite hydrogel	1221:1253	the fabricated composite hydrogel	1221:1253	The results showed that the fabricated composite hydrogel had a uniform porous architecture, excellent fluid absorbability, and appropriate mechanical stability.					
36162531	5	61	dep	in	1088:1089	arg1	vitro					1091:1095	vitro	1091:1095	vitro	1091:1095	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	7	62	theme	healing	1772:1778	arg1	application					1780:1790	chronic wound healing application	1758:1790	chronic wound healing application	1758:1790	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	5	63	theme	healing	1133:1139	arg1	properties					1141:1150	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties	1024:1150	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels	1024:1163	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	1	64	theme	mechanical	267:276	arg1	properties					304:313	appropriate mechanical, biological, and physical properties	255:313	appropriate mechanical, biological, and physical properties	255:313	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	7	65	theme	chronic	1758:1764	arg1	healing					1772:1778	chronic wound healing	1758:1778	chronic wound healing application	1758:1790	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	2	66	theme	cross-linkable	552:565	arg1	acid					578:581	photo cross-linkable hyaluronic acid	546:581	photo cross-linkable hyaluronic acid	546:581	In the present study, chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA) was synthesized using H2O2-induced grafting polymerization, and photo cross-linkable hyaluronic acid was synthesized using methacrilation (HAMA).					
36162531	7	67	theme	hydrogel	1497:1504	arg1	antioxidant					1448:1458	antioxidant	1448:1458	antioxidant	1448:1458	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	7	67	theme	hydrogel	1497:1504	arg1	biocompatibility					1430:1445	the in vitro biocompatibility	1417:1445	the in vitro biocompatibility	1417:1445	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	7	67	theme	hydrogel	1497:1504	arg1	properties					1479:1488	antimicrobial properties	1465:1488	antimicrobial properties	1465:1488	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	1	68	theme	Wound	143:147	arg1	hydrogel					158:165	Wound dressing hydrogel	143:165	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties	143:313	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	6	69	theme	fluid	1300:1304	arg1	absorbability					1306:1318	excellent fluid absorbability	1290:1318	excellent fluid absorbability	1290:1318	The results showed that the fabricated composite hydrogel had a uniform porous architecture, excellent fluid absorbability, and appropriate mechanical stability.					
36162531	4	70	theme	duel	842:845	arg1	hydrogel					899:906	duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel	842:906	duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application	842:1021	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	3	71	theme	COS-SA	645:650	arg1	synthesis					632:640	The synthesis	628:640	The synthesis of COS-SA and HAMA	628:659	The synthesis of COS-SA and HAMA was confirmed by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and polyphenol assay.					
36162531	4	72	theme	antimicrobial	955:967	arg1	dressing					969:976	antimicrobial dressing	955:976	antimicrobial dressing for full-thickness wound healing application	955:1021	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	7	73	theme	antimicrobial	1465:1477	arg1	properties					1479:1488	antimicrobial properties	1465:1488	antimicrobial properties	1465:1488	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	0	74	theme	hyaluronic	68:77	arg1	hydrogel					133:140	hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel	68:140	hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel	68:140	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	1	75	theme	multifunctional	172:186	arg1	properties					188:197	multifunctional properties	172:197	multifunctional properties	172:197	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	1	75	theme	multifunctional	172:186	arg1	properties					240:249	antioxidant and antimicrobial properties	210:249	antioxidant and antimicrobial properties	210:249	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	1	75	theme	multifunctional	172:186	arg1	properties					304:313	appropriate mechanical, biological, and physical properties	255:313	appropriate mechanical, biological, and physical properties	255:313	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	7	76	theme	wound	1713:1717	arg1	dressing					1719:1726	COSs-SA incorporated PVA/HAMA hydrogel wound dressing	1674:1726	COSs-SA incorporated PVA/HAMA hydrogel wound dressing	1674:1726	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	2	77	theme	H2O2-induced	504:515	arg1	polymerization					526:539	H2O2-induced grafting polymerization	504:539	H2O2-induced grafting polymerization	504:539	In the present study, chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA) was synthesized using H2O2-induced grafting polymerization, and photo cross-linkable hyaluronic acid was synthesized using methacrilation (HAMA).					
36162531	6	78	theme	porous	1269:1274	arg1	architecture					1276:1287	a uniform porous architecture	1259:1287	a uniform porous architecture	1259:1287	The results showed that the fabricated composite hydrogel had a uniform porous architecture, excellent fluid absorbability, and appropriate mechanical stability.					
36162531	5	79	dep	chemical	1028:1035	arg1	physical					1038:1045	physical	1038:1045	physical	1038:1045	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	5	79	dep	chemical	1028:1035	arg1	antioxidant					1060:1070	antioxidant	1060:1070	antioxidant	1060:1070	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	5	79	dep	chemical	1028:1035	arg1	mechanical					1048:1057	mechanical	1048:1057	mechanical	1048:1057	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	0	80	theme	acid	113:116	arg1	hydrogel					133:140	hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel	68:140	hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel	68:140	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	7	81	theme	PVA/HAMA	1695:1702	arg1	dressing					1719:1726	COSs-SA incorporated PVA/HAMA hydrogel wound dressing	1674:1726	COSs-SA incorporated PVA/HAMA hydrogel wound dressing	1674:1726	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	0	82	theme	dressing	124:131	arg1	hydrogel					133:140	hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel	68:140	hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel	68:140	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	1	83	with	hydrogel	158:165	arg1	properties					188:197	multifunctional properties	172:197	multifunctional properties	172:197	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	1	83	with	hydrogel	158:165	arg1	properties					240:249	antioxidant and antimicrobial properties	210:249	antioxidant and antimicrobial properties	210:249	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	1	83	with	hydrogel	158:165	arg1	properties					304:313	appropriate mechanical, biological, and physical properties	255:313	appropriate mechanical, biological, and physical properties	255:313	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	0	84	theme	dual	16:19	arg1	poly					34:37	Multifunctional dual cross-linked poly	0:37	Multifunctional dual cross-linked poly (vinyl alcohol)	0:53	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	0	84	theme	dual	16:19	arg1	alcohol					46:52	vinyl alcohol	40:52	vinyl alcohol	40:52	Multifunctional dual cross-linked poly (vinyl alcohol)/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid wound dressing hydrogel.					
36162531	7	85	theme	in	1421:1422	arg1	biocompatibility					1430:1445	the in vitro biocompatibility	1417:1445	the in vitro biocompatibility	1417:1445	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	2	86	theme	hyaluronic	567:576	arg1	acid					578:581	photo cross-linkable hyaluronic acid	546:581	photo cross-linkable hyaluronic acid	546:581	In the present study, chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA) was synthesized using H2O2-induced grafting polymerization, and photo cross-linkable hyaluronic acid was synthesized using methacrilation (HAMA).					
36162531	2	87	theme	sinapic	445:451	arg1	SA					459:460	SA	459:460	SA	459:460	In the present study, chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA) was synthesized using H2O2-induced grafting polymerization, and photo cross-linkable hyaluronic acid was synthesized using methacrilation (HAMA).					
36162531	2	87	theme	sinapic	445:451	arg1	acid					453:456	sinapic acid	445:456	chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA)	416:480	In the present study, chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA) was synthesized using H2O2-induced grafting polymerization, and photo cross-linkable hyaluronic acid was synthesized using methacrilation (HAMA).					
36162531	1	88	from	challenge	383:391	arg1	application					353:363	wound healing application	339:363	wound healing application	339:363	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	1	89	theme	healing	345:351	arg1	application					353:363	wound healing application	339:363	wound healing application	339:363	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
36162531	7	90	dep	in	1421:1422	arg1	vitro					1424:1428	vitro	1424:1428	vitro	1424:1428	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	7	91	theme	conjugate	1387:1395	arg1	introduction					1363:1374	The introduction	1359:1374	The introduction of COSs-SA conjugate	1359:1395	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	3	92	theme	HAMA	656:659	arg1	synthesis					632:640	The synthesis	628:640	The synthesis of COS-SA and HAMA	628:659	The synthesis of COS-SA and HAMA was confirmed by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and polyphenol assay.					
36162531	4	93	theme	alcohol	870:876	arg1	hydrogel					899:906	duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel	842:906	duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application	842:1021	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	7	94	theme	deposition	1647:1656	arg1	promotion					1534:1542	the significant promotion	1518:1542	the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application	1518:1790	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	4	95	theme	wound	997:1001	arg1	healing					1003:1009	full-thickness wound healing	982:1009	full-thickness wound healing application	982:1021	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	5	96	theme	antimicrobial	1073:1085	arg1	properties					1141:1150	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties	1024:1150	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels	1024:1163	The chemical, physical, mechanical, antioxidant, antimicrobial, in vitro biocompatibility, and in vivo wound healing properties of hydrogels were subsequently investigated.					
36162531	3	97	theme	Fourier-transform	678:694	arg1	spectroscopy					705:716	Fourier-transform infrared spectroscopy	678:716	Fourier-transform infrared spectroscopy	678:716	The synthesis of COS-SA and HAMA was confirmed by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and polyphenol assay.					
36162531	7	98	theme	formation	1623:1631	arg1	promotion					1534:1542	the significant promotion	1518:1542	the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application	1518:1790	The introduction of COSs-SA conjugate remarkably enhanced the in vitro biocompatibility, antioxidant, and antimicrobial properties of the hydrogel, leading to the significant promotion of in vivo full-thickness wound closure, re-epithelization, granulation tissue formation, and collagen deposition indicating that COSs-SA incorporated PVA/HAMA hydrogel wound dressing has significant potential for chronic wound healing application.					
36162531	3	99	theme	magnetic	734:741	arg1	resonance					743:751	proton nuclear magnetic resonance	719:751	proton nuclear magnetic resonance spectroscopy	719:764	The synthesis of COS-SA and HAMA was confirmed by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and polyphenol assay.					
36162531	4	100	theme	cross-linked	847:858	arg1	PVA					879:881	PVA	879:881	PVA	879:881	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	4	100	theme	cross-linked	847:858	arg1	alcohol					870:876	cross-linked polyvinyl alcohol	847:876	duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application	842:1021	Subsequently, we developed duel cross-linked polyvinyl alcohol (PVA)/HAMA composite hydrogel encapsulated with COS-SA as an antioxidant and antimicrobial dressing for full-thickness wound healing application.					
36162531	2	101	theme	present	401:407	arg1	study					409:413	the present study	397:413	the present study	397:413	In the present study, chitooligosaccharides (COS)/ sinapic acid (SA) conjugate (COS-SA) was synthesized using H2O2-induced grafting polymerization, and photo cross-linkable hyaluronic acid was synthesized using methacrilation (HAMA).					
36162531	1	102	theme	physical	295:302	arg1	properties					304:313	appropriate mechanical, biological, and physical properties	255:313	appropriate mechanical, biological, and physical properties	255:313	Wound dressing hydrogel with multifunctional properties, including antioxidant and antimicrobial properties and appropriate mechanical, biological, and physical properties is of great interest in wound healing application and it is still a challenge.					
37230566	0	0	theme	hydrolysate	31:41	arg1	profiles					43:50	protein hydrolysate profiles	23:50	protein hydrolysate profiles	23:50	Strategies for mapping protein hydrolysate profiles and pharmacokinetics based on non-targeted proteomics combining skyline-aided quantitative techniques.					
37230566	4	1	from	matrix	836:841	arg1	LP					817:818	LP	817:818	LP from biological matrix	817:841	First, solvents with polarity gradients were used to globally extract peptides of LP from biological matrix.					
37230566	4	1	from	matrix	836:841	arg1	peptides					805:812	extract peptides	797:812	extract peptides of LP from biological matrix	797:841	First, solvents with polarity gradients were used to globally extract peptides of LP from biological matrix.					
37230566	7	2	theme	LPs	1259:1261	arg1	parameters					1245:1254	the LC-MS/MS detection parameters	1222:1254	the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay	1222:1349	In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay.					
37230566	7	3	theme	quantitative	1140:1151	arg1	workflow					1162:1169	the quantitative analysis workflow	1136:1169	the quantitative analysis workflow	1136:1169	In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay.					
37230566	1	4	theme	authentic	452:460	arg1	standards					462:470	authentic standards	452:470	authentic standards	452:470	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	1	5	theme	drug	272:275	arg1	formulations					277:288	food or drug formulations	264:288	food or drug formulations	264:288	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	10	6	theme	established	1617:1627	arg1	assays					1658:1663	The established qualitative and quantitative assays	1613:1663	The established qualitative and quantitative assays	1613:1663	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	10	6	theme	established	1617:1627	arg1	conductive					1762:1771	conductive	1762:1771	conductive	1762:1771	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	10	7	theme	various	1843:1849	arg1	PHs					1851:1853	various PHs	1843:1853	various PHs	1843:1853	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	1	8	theme	hydrolysates	220:231	arg1	bioactivities					195:207	the bioactivities	191:207	the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations	191:288	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	10	9	theme	peptides	1831:1838	arg1	pharmacokinetics					1811:1826	pharmacokinetics	1811:1826	pharmacokinetics	1811:1826	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	10	9	theme	peptides	1831:1838	arg1	profile					1799:1805	profile	1799:1805	profile	1799:1805	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	2	10	theme	sample	576:581	arg1	preparation					583:593	sample preparation	576:593	sample preparation	576:593	The present study aims to develop systematic analytical strategy and technical platform with optimized sample preparation, separation and detection protocols for PHs.					
37230566	3	11	theme	Lineal	640:645	arg1	cases					728:732	cases	728:732	cases	728:732	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	3	11	theme	Lineal	640:645	arg1	LPs					657:659	LPs	657:659	LPs	657:659	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	3	11	theme	Lineal	640:645	arg1	extraction					663:672	extraction	663:672	extraction of the spleen of healthy pigs or calves	663:712	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	3	11	theme	Lineal	640:645	arg1	peptides					647:654	Lineal peptides	640:654	Lineal peptides (LPs)	640:660	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	8	12	theme	authentic	1487:1495	arg1	standards					1497:1505	authentic standards	1487:1505	authentic standards	1487:1505	Note worthily, we innovatively prepared calibration curves by sequential dilution of LP solution to overcome the bottleneck of lacking authentic standards and complex PH composition.					
37230566	1	13	theme	Numerous	155:162	arg1	works					164:168	Numerous works	155:168	Numerous works	155:168	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	1	14	from	application	249:259	arg1	formulations					277:288	food or drug formulations	264:288	food or drug formulations	264:288	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	4	15	from	peptides	805:812	arg1	matrix					836:841	biological matrix	825:841	biological matrix	825:841	First, solvents with polarity gradients were used to globally extract peptides of LP from biological matrix.					
37230566	5	16	theme	reliable	929:936	arg1	analysis					950:957	a reliable qualitative analysis	927:957	a reliable qualitative analysis workflow for PHs	927:974	Non-targeted proteomics based on a high-resolution MS system was used to establish a reliable qualitative analysis workflow for PHs.					
37230566	8	17	theme	PH	1519:1520	arg1	composition					1522:1532	complex PH composition	1511:1532	complex PH composition	1511:1532	Note worthily, we innovatively prepared calibration curves by sequential dilution of LP solution to overcome the bottleneck of lacking authentic standards and complex PH composition.					
37230566	1	18	from	bioactivities	195:207	arg1	formulations					277:288	food or drug formulations	264:288	food or drug formulations	264:288	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	9	19	theme	good	1562:1565	arg1	linearity					1567:1575	linearity	1567:1575	linearity	1567:1575	All the peptides exhibited good linearity and precision in biological matrix.					
37230566	2	20	theme	systematic	507:516	arg1	strategy					529:536	systematic analytical strategy	507:536	systematic analytical strategy	507:536	The present study aims to develop systematic analytical strategy and technical platform with optimized sample preparation, separation and detection protocols for PHs.					
37230566	0	21	theme	non-targeted	82:93	arg1	proteomics					95:104	non-targeted proteomics	82:104	non-targeted proteomics combining skyline-aided quantitative techniques	82:152	Strategies for mapping protein hydrolysate profiles and pharmacokinetics based on non-targeted proteomics combining skyline-aided quantitative techniques.					
37230566	10	22	from	PHs	1851:1853	arg1	pharmacokinetics					1811:1826	pharmacokinetics	1811:1826	pharmacokinetics	1811:1826	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	10	22	from	PHs	1851:1853	arg1	profile					1799:1805	profile	1799:1805	profile	1799:1805	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	2	23	with	strategy	529:536	arg1	protocols					621:629	optimized sample preparation, separation and detection protocols	566:629	optimized sample preparation, separation and detection protocols	566:629	The present study aims to develop systematic analytical strategy and technical platform with optimized sample preparation, separation and detection protocols for PHs.					
37230566	7	24	dep	linearity	1293:1301	arg1	the					1289:1291	the	1289:1291	the	1289:1291	In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay.					
37230566	2	25	theme	technical	542:550	arg1	platform					552:559	technical platform	542:559	technical platform	542:559	The present study aims to develop systematic analytical strategy and technical platform with optimized sample preparation, separation and detection protocols for PHs.					
37230566	0	26	theme	quantitative	130:141	arg1	techniques					143:152	skyline-aided quantitative techniques	116:152	skyline-aided quantitative techniques	116:152	Strategies for mapping protein hydrolysate profiles and pharmacokinetics based on non-targeted proteomics combining skyline-aided quantitative techniques.					
37230566	10	27	theme	distribution	1704:1715	arg1	characteristics					1717:1731	the distribution characteristics	1700:1731	the distribution characteristics of LPs	1700:1738	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	8	28	theme	sequential	1414:1423	arg1	dilution					1425:1432	sequential dilution	1414:1432	sequential dilution of LP solution	1414:1447	Note worthily, we innovatively prepared calibration curves by sequential dilution of LP solution to overcome the bottleneck of lacking authentic standards and complex PH composition.					
37230566	7	29	theme	analytical	1334:1343	arg1	assay					1345:1349	the developed analytical assay	1320:1349	the developed analytical assay	1320:1349	In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay.					
37230566	4	30	theme	LP	817:818	arg1	peptides					805:812	extract peptides	797:812	extract peptides of LP from biological matrix	797:841	First, solvents with polarity gradients were used to globally extract peptides of LP from biological matrix.					
37230566	7	31	theme	Skyline	1172:1178	arg1	software					1180:1187	Skyline software	1172:1187	Skyline software	1172:1187	In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay.					
37230566	7	32	used	used	1193:1196	arg2	software					1180:1187	Skyline software	1172:1187	Skyline software	1172:1187	In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay.					
37230566	4	33	theme	biological	825:834	arg1	matrix					836:841	biological matrix	825:841	biological matrix	825:841	First, solvents with polarity gradients were used to globally extract peptides of LP from biological matrix.					
37230566	3	34	theme	pigs	699:702	arg1	spleen					681:686	the spleen	677:686	the spleen of healthy pigs or calves	677:712	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	4	35	used	used	780:783	arg2	solvents					742:749	solvents	742:749	solvents with polarity gradients	742:773	First, solvents with polarity gradients were used to globally extract peptides of LP from biological matrix.					
37230566	6	36	theme	MicroLC-Q-TOF/MS	1108:1123	arg1	system					1125:1130	the MicroLC-Q-TOF/MS system	1104:1130	the MicroLC-Q-TOF/MS system	1104:1130	Based on the developed approach, 247 unique peptides were identified using NanoLC-Orbitrap-MS/MS, and then further verified on the MicroLC-Q-TOF/MS system.					
37230566	3	37	used	used	720:723	arg2	cases					728:732	cases	728:732	cases	728:732	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	3	37	used	used	720:723	arg2	LPs					657:659	LPs	657:659	LPs	657:659	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	3	37	used	used	720:723	arg2	peptides					647:654	Lineal peptides	640:654	Lineal peptides (LPs)	640:660	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	3	37	used	used	720:723	arg2	extraction					663:672	extraction	663:672	extraction of the spleen of healthy pigs or calves	663:712	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	10	38	theme	qualitative	1629:1639	arg1	assays					1658:1663	The established qualitative and quantitative assays	1613:1663	The established qualitative and quantitative assays	1613:1663	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	10	38	theme	qualitative	1629:1639	arg1	conductive					1762:1771	conductive	1762:1771	conductive	1762:1771	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	3	39	theme	calves	707:712	arg1	spleen					681:686	the spleen	677:686	the spleen of healthy pigs or calves	677:712	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	1	40	theme	standards	462:470	arg1	half-life					400:408	short half-life	394:408	short half-life	394:408	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	1	40	theme	standards	462:470	arg1	lack					444:447	lack	444:447	lack of authentic standards	444:470	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	1	40	theme	standards	462:470	arg1	complex					373:379	complex	373:379	complex	373:379	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	1	40	theme	standards	462:470	arg1	concentrations					425:438	extremely low concentrations	411:438	extremely low concentrations	411:438	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	7	41	theme	detection	1235:1243	arg1	parameters					1245:1254	the LC-MS/MS detection parameters	1222:1254	the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay	1222:1349	In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay.					
37230566	1	42	theme	protein	212:218	arg1	PHs					234:236	PHs	234:236	PHs	234:236	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	1	42	theme	protein	212:218	arg1	hydrolysates					220:231	protein hydrolysates	212:231	protein hydrolysates (PHs)	212:237	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	10	43	theme	quantitative	1645:1656	arg1	assays					1658:1663	The established qualitative and quantitative assays	1613:1663	The established qualitative and quantitative assays	1613:1663	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	10	43	theme	quantitative	1645:1656	arg1	conductive					1762:1771	conductive	1762:1771	conductive	1762:1771	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	1	44	from	formulations	277:288	arg1	bioactivities					195:207	the bioactivities	191:207	the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations	191:288	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	2	45	theme	preparation	583:593	arg1	protocols					621:629	optimized sample preparation, separation and detection protocols	566:629	optimized sample preparation, separation and detection protocols	566:629	The present study aims to develop systematic analytical strategy and technical platform with optimized sample preparation, separation and detection protocols for PHs.					
37230566	8	46	theme	lacking	1479:1485	arg1	standards					1497:1505	authentic standards	1487:1505	authentic standards	1487:1505	Note worthily, we innovatively prepared calibration curves by sequential dilution of LP solution to overcome the bottleneck of lacking authentic standards and complex PH composition.					
37230566	5	47	used	used	909:912	arg2	proteomics					857:866	Non-targeted proteomics	844:866	Non-targeted proteomics based on a high-resolution MS system	844:903	Non-targeted proteomics based on a high-resolution MS system was used to establish a reliable qualitative analysis workflow for PHs.					
37230566	5	48	theme	high-resolution	879:893	arg1	system					898:903	a high-resolution MS system	877:903	a high-resolution MS system	877:903	Non-targeted proteomics based on a high-resolution MS system was used to establish a reliable qualitative analysis workflow for PHs.					
37230566	5	49	theme	Non-targeted	844:855	arg1	proteomics					857:866	Non-targeted proteomics	844:866	Non-targeted proteomics based on a high-resolution MS system	844:903	Non-targeted proteomics based on a high-resolution MS system was used to establish a reliable qualitative analysis workflow for PHs.					
37230566	2	50	theme	optimized	566:574	arg1	protocols					621:629	optimized sample preparation, separation and detection protocols	566:629	optimized sample preparation, separation and detection protocols	566:629	The present study aims to develop systematic analytical strategy and technical platform with optimized sample preparation, separation and detection protocols for PHs.					
37230566	8	51	theme	standards	1497:1505	arg1	bottleneck					1465:1474	the bottleneck	1461:1474	the bottleneck of lacking authentic standards and complex PH composition	1461:1532	Note worthily, we innovatively prepared calibration curves by sequential dilution of LP solution to overcome the bottleneck of lacking authentic standards and complex PH composition.					
37230566	4	52	with	solvents	742:749	arg1	gradients					765:773	polarity gradients	756:773	polarity gradients	756:773	First, solvents with polarity gradients were used to globally extract peptides of LP from biological matrix.					
37230566	1	53	theme	low	421:423	arg1	complex					373:379	complex	373:379	complex	373:379	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	1	53	theme	low	421:423	arg1	concentrations					425:438	extremely low concentrations	411:438	extremely low concentrations	411:438	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	8	54	theme	solution	1440:1447	arg1	dilution					1425:1432	sequential dilution	1414:1432	sequential dilution of LP solution	1414:1447	Note worthily, we innovatively prepared calibration curves by sequential dilution of LP solution to overcome the bottleneck of lacking authentic standards and complex PH composition.					
37230566	2	55	theme	detection	611:619	arg1	protocols					621:629	optimized sample preparation, separation and detection protocols	566:629	optimized sample preparation, separation and detection protocols	566:629	The present study aims to develop systematic analytical strategy and technical platform with optimized sample preparation, separation and detection protocols for PHs.					
37230566	8	56	theme	complex	1511:1517	arg1	composition					1522:1532	complex PH composition	1511:1532	complex PH composition	1511:1532	Note worthily, we innovatively prepared calibration curves by sequential dilution of LP solution to overcome the bottleneck of lacking authentic standards and complex PH composition.					
37230566	10	57	from	profile	1799:1805	arg1	PHs					1851:1853	various PHs	1843:1853	various PHs	1843:1853	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	2	58	theme	separation	596:605	arg1	protocols					621:629	optimized sample preparation, separation and detection protocols	566:629	optimized sample preparation, separation and detection protocols	566:629	The present study aims to develop systematic analytical strategy and technical platform with optimized sample preparation, separation and detection protocols for PHs.					
37230566	8	59	theme	composition	1522:1532	arg1	bottleneck					1465:1474	the bottleneck	1461:1474	the bottleneck of lacking authentic standards and complex PH composition	1461:1532	Note worthily, we innovatively prepared calibration curves by sequential dilution of LP solution to overcome the bottleneck of lacking authentic standards and complex PH composition.					
37230566	1	60	from	hydrolysates	220:231	arg1	formulations					277:288	food or drug formulations	264:288	food or drug formulations	264:288	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	5	61	theme	MS	895:896	arg1	system					898:903	a high-resolution MS system	877:903	a high-resolution MS system	877:903	Non-targeted proteomics based on a high-resolution MS system was used to establish a reliable qualitative analysis workflow for PHs.					
37230566	10	62	from	pharmacokinetics	1811:1826	arg1	PHs					1851:1853	various PHs	1843:1853	various PHs	1843:1853	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	6	63	theme	unique	1014:1019	arg1	peptides					1021:1028	247 unique peptides	1010:1028	247 unique peptides	1010:1028	Based on the developed approach, 247 unique peptides were identified using NanoLC-Orbitrap-MS/MS, and then further verified on the MicroLC-Q-TOF/MS system.					
37230566	2	64	theme	analytical	518:527	arg1	strategy					529:536	systematic analytical strategy	507:536	systematic analytical strategy	507:536	The present study aims to develop systematic analytical strategy and technical platform with optimized sample preparation, separation and detection protocols for PHs.					
37230566	10	65	dep	profile	1799:1805	arg1	the					1795:1797	the	1795:1797	the	1795:1797	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	7	66	theme	LC-MS/MS	1226:1233	arg1	parameters					1245:1254	the LC-MS/MS detection parameters	1222:1254	the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay	1222:1349	In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay.					
37230566	10	67	from	peptides	1831:1838	arg1	PHs					1851:1853	various PHs	1843:1853	various PHs	1843:1853	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	0	68	theme	skyline-aided	116:128	arg1	techniques					143:152	skyline-aided quantitative techniques	116:152	skyline-aided quantitative techniques	116:152	Strategies for mapping protein hydrolysate profiles and pharmacokinetics based on non-targeted proteomics combining skyline-aided quantitative techniques.					
37230566	6	69	theme	developed	990:998	arg1	approach					1000:1007	the developed approach	986:1007	the developed approach	986:1007	Based on the developed approach, 247 unique peptides were identified using NanoLC-Orbitrap-MS/MS, and then further verified on the MicroLC-Q-TOF/MS system.					
37230566	4	70	theme	polarity	756:763	arg1	gradients					765:773	polarity gradients	756:773	polarity gradients	756:773	First, solvents with polarity gradients were used to globally extract peptides of LP from biological matrix.					
37230566	6	71	dep	identified	1035:1044	arg1	verified					1092:1099	verified	1092:1099	verified on the MicroLC-Q-TOF/MS system	1092:1130	Based on the developed approach, 247 unique peptides were identified using NanoLC-Orbitrap-MS/MS, and then further verified on the MicroLC-Q-TOF/MS system.					
37230566	9	72	theme	biological	1594:1603	arg1	matrix					1605:1610	biological matrix	1594:1610	biological matrix	1594:1610	All the peptides exhibited good linearity and precision in biological matrix.					
37230566	1	73	theme	short	394:398	arg1	half-life					400:408	short half-life	394:408	short half-life	394:408	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	1	73	theme	short	394:398	arg1	complex					373:379	complex	373:379	complex	373:379	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	5	74	theme	qualitative	938:948	arg1	analysis					950:957	a reliable qualitative analysis	927:957	a reliable qualitative analysis workflow for PHs	927:974	Non-targeted proteomics based on a high-resolution MS system was used to establish a reliable qualitative analysis workflow for PHs.					
37230566	7	75	theme	assay	1345:1349	arg1	linearity					1293:1301	linearity	1293:1301	linearity	1293:1301	In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay.					
37230566	7	75	theme	assay	1345:1349	arg1	precision					1307:1315	precision	1307:1315	precision	1307:1315	In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay.					
37230566	8	76	theme	calibration	1392:1402	arg1	curves					1404:1409	calibration curves	1392:1409	calibration curves	1392:1409	Note worthily, we innovatively prepared calibration curves by sequential dilution of LP solution to overcome the bottleneck of lacking authentic standards and complex PH composition.					
37230566	0	77	theme	protein	23:29	arg1	profiles					43:50	protein hydrolysate profiles	23:50	protein hydrolysate profiles	23:50	Strategies for mapping protein hydrolysate profiles and pharmacokinetics based on non-targeted proteomics combining skyline-aided quantitative techniques.					
37230566	2	78	with	platform	552:559	arg1	protocols					621:629	optimized sample preparation, separation and detection protocols	566:629	optimized sample preparation, separation and detection protocols	566:629	The present study aims to develop systematic analytical strategy and technical platform with optimized sample preparation, separation and detection protocols for PHs.					
37230566	5	79	theme	workflow	959:966	arg1	analysis					950:957	a reliable qualitative analysis	927:957	a reliable qualitative analysis workflow for PHs	927:974	Non-targeted proteomics based on a high-resolution MS system was used to establish a reliable qualitative analysis workflow for PHs.					
37230566	7	80	theme	developed	1324:1332	arg1	assay					1345:1349	the developed analytical assay	1320:1349	the developed analytical assay	1320:1349	In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay.					
37230566	8	81	dep	worthily	1357:1364	arg1	Note					1352:1355	Note	1352:1355	Note	1352:1355	Note worthily, we innovatively prepared calibration curves by sequential dilution of LP solution to overcome the bottleneck of lacking authentic standards and complex PH composition.					
37230566	10	82	theme	LPs	1736:1738	arg1	characteristics					1717:1731	the distribution characteristics	1700:1731	the distribution characteristics of LPs	1700:1738	The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro.					
37230566	7	83	theme	analysis	1153:1160	arg1	workflow					1162:1169	the quantitative analysis workflow	1136:1169	the quantitative analysis workflow	1136:1169	In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay.					
37230566	2	84	theme	present	477:483	arg1	study					485:489	The present study	473:489	The present study	473:489	The present study aims to develop systematic analytical strategy and technical platform with optimized sample preparation, separation and detection protocols for PHs.					
37230566	1	85	dep	complex	373:379	arg1	constitutes					381:391	constitutes	381:391	constitutes	381:391	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	3	86	theme	healthy	691:697	arg1	pigs					699:702	healthy pigs	691:702	healthy pigs	691:702	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	8	87	theme	LP	1437:1438	arg1	solution					1440:1447	LP solution	1437:1447	LP solution	1437:1447	Note worthily, we innovatively prepared calibration curves by sequential dilution of LP solution to overcome the bottleneck of lacking authentic standards and complex PH composition.					
37230566	3	88	theme	spleen	681:686	arg1	extraction					663:672	extraction	663:672	extraction of the spleen of healthy pigs or calves	663:712	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	3	88	theme	spleen	681:686	arg1	peptides					647:654	Lineal peptides	640:654	Lineal peptides (LPs)	640:660	Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases.					
37230566	1	89	theme	application	249:259	arg1	bioactivities					195:207	the bioactivities	191:207	the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations	191:288	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
37230566	4	90	theme	extract	797:803	arg1	peptides					805:812	extract peptides	797:812	extract peptides of LP from biological matrix	797:841	First, solvents with polarity gradients were used to globally extract peptides of LP from biological matrix.					
37230566	1	91	theme	food	264:267	arg1	formulations					277:288	food or drug formulations	264:288	food or drug formulations	264:288	Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards.					
35537582	6	0	theme	thermal	674:680	arg1	stability					682:690	the thermal stability	670:690	the thermal stability	670:690	DSC confirmed MW did affect the thermal stability.					
35537582	10	1	theme	molecular	967:975	arg1	weight					977:982	chitosan molecular weight	958:982	chitosan molecular weight	958:982	Hence, chitosan molecular weight must be chosen carefully according to its application and incorporation of protein hydrolysate into film would be an efficient strategy to modify film performance.					
35537582	9	2	theme	antibacterial	892:904	arg1	activity					922:929	both antibacterial and antioxidant activity	887:929	both antibacterial and antioxidant activity of the CH/RH films	887:948	Curcumin effectively improved both antibacterial and antioxidant activity of the CH/RH films.					
35537582	8	3	theme	antibacterial	798:810	arg1	activity					812:819	higher antibacterial activity	791:819	higher antibacterial activity	791:819	The 30 kDa CH/RH film exhibited higher antibacterial activity against both E. coli and S. aureus.					
35537582	6	4	dep	confirmed	646:654	arg1	affect					663:668	affect	663:668	confirmed MW did affect the thermal stability	646:690	DSC confirmed MW did affect the thermal stability.					
35537582	9	5	theme	antioxidant	910:920	arg1	activity					922:929	both antibacterial and antioxidant activity	887:929	both antibacterial and antioxidant activity of the CH/RH films	887:948	Curcumin effectively improved both antibacterial and antioxidant activity of the CH/RH films.					
35537582	10	6	theme	hydrolysate	1067:1077	arg1	strategy					1111:1118	an efficient strategy	1098:1118	an efficient strategy to modify film performance	1098:1145	Hence, chitosan molecular weight must be chosen carefully according to its application and incorporation of protein hydrolysate into film would be an efficient strategy to modify film performance.					
35537582	10	6	theme	hydrolysate	1067:1077	arg1	incorporation					1042:1054	incorporation	1042:1054	incorporation of protein hydrolysate into film	1042:1087	Hence, chitosan molecular weight must be chosen carefully according to its application and incorporation of protein hydrolysate into film would be an efficient strategy to modify film performance.					
35537582	5	7	theme	extraordinary	581:593	arg1	extensibility					595:607	extraordinary extensibility	581:607	extraordinary extensibility ranging from 180.26% to 204.08%	581:639	All films showed extraordinary extensibility ranging from 180.26% to 204.08%.					
35537582	3	8	theme	tensile	471:477	arg1	strength					479:486	tensile strength	471:486	tensile strength	471:486	With increasing MW (<100 kDa), moisture, solubility and water vapor transmission marginally decreased while tensile strength increased.					
35537582	7	9	dep	activity	730:737	arg1	MW					703:704	The lower MW	693:704	The lower MW	693:704	The lower MW, the higher antioxidant activity of the CH/RH films.					
35537582	9	10	theme	films	944:948	arg1	activity					922:929	both antibacterial and antioxidant activity	887:929	both antibacterial and antioxidant activity of the CH/RH films	887:948	Curcumin effectively improved both antibacterial and antioxidant activity of the CH/RH films.					
35537582	0	11	theme	hydrolysate/curcumin	14:33	arg1	film					45:48	Chitosan/rice hydrolysate/curcumin composite film	0:48	Chitosan/rice hydrolysate/curcumin composite film: Effect of chitosan molecular weight.	0:86	Chitosan/rice hydrolysate/curcumin composite film: Effect of chitosan molecular weight.					
35537582	1	12	theme	composite	92:100	arg1	films					102:106	The composite films	88:106	The composite films	88:106	The composite films were prepared by adding rice protein hydrolysate (RH) into chitosan (CH).					
35537582	0	13	theme	Chitosan/rice	0:12	arg1	film					45:48	Chitosan/rice hydrolysate/curcumin composite film	0:48	Chitosan/rice hydrolysate/curcumin composite film: Effect of chitosan molecular weight.	0:86	Chitosan/rice hydrolysate/curcumin composite film: Effect of chitosan molecular weight.					
35537582	7	14	theme	films	752:756	arg1	activity					730:737	the higher antioxidant activity	707:737	the higher antioxidant activity of the CH/RH films	707:756	The lower MW, the higher antioxidant activity of the CH/RH films.					
35537582	2	15	dep	MW	235:236	arg1	300 kDa					258:264	300 kDa	258:264	300 kDa	258:264	Effect of four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa) was evaluated and curcumin was further incorporated into the film to enhance its bioactivities.					
35537582	9	16	theme	CH/RH	938:942	arg1	films					944:948	the CH/RH films	934:948	the CH/RH films	934:948	Curcumin effectively improved both antibacterial and antioxidant activity of the CH/RH films.					
35537582	4	17	theme	Rice	499:502	arg1	hydrolysate					504:514	Rice hydrolysate	499:514	Rice hydrolysate	499:514	Rice hydrolysate improved the film solubility and extensibility.					
35537582	0	18	theme	composite	35:43	arg1	film					45:48	Chitosan/rice hydrolysate/curcumin composite film	0:48	Chitosan/rice hydrolysate/curcumin composite film: Effect of chitosan molecular weight.	0:86	Chitosan/rice hydrolysate/curcumin composite film: Effect of chitosan molecular weight.					
35537582	7	19	theme	CH/RH	746:750	arg1	films					752:756	the CH/RH films	742:756	the CH/RH films	742:756	The lower MW, the higher antioxidant activity of the CH/RH films.					
35537582	2	20	theme	molecular	216:224	arg1	MW					235:236	MW	235:236	MW	235:236	Effect of four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa) was evaluated and curcumin was further incorporated into the film to enhance its bioactivities.					
35537582	2	20	theme	molecular	216:224	arg1	weights					226:232	four different chitosan molecular weights	192:232	four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa)	192:265	Effect of four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa) was evaluated and curcumin was further incorporated into the film to enhance its bioactivities.					
35537582	2	21	theme	chitosan	207:214	arg1	MW					235:236	MW	235:236	MW	235:236	Effect of four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa) was evaluated and curcumin was further incorporated into the film to enhance its bioactivities.					
35537582	2	21	theme	chitosan	207:214	arg1	weights					226:232	four different chitosan molecular weights	192:232	four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa)	192:265	Effect of four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa) was evaluated and curcumin was further incorporated into the film to enhance its bioactivities.					
35537582	10	22	theme	efficient	1101:1109	arg1	strategy					1111:1118	an efficient strategy	1098:1118	an efficient strategy to modify film performance	1098:1145	Hence, chitosan molecular weight must be chosen carefully according to its application and incorporation of protein hydrolysate into film would be an efficient strategy to modify film performance.					
35537582	10	22	theme	efficient	1101:1109	arg1	incorporation					1042:1054	incorporation	1042:1054	incorporation of protein hydrolysate into film	1042:1087	Hence, chitosan molecular weight must be chosen carefully according to its application and incorporation of protein hydrolysate into film would be an efficient strategy to modify film performance.					
35537582	0	23	theme	chitosan	61:68	arg1	weight					80:85	chitosan molecular weight	61:85	chitosan molecular weight	61:85	Chitosan/rice hydrolysate/curcumin composite film: Effect of chitosan molecular weight.					
35537582	4	24	dep	film	529:532	arg1	extensibility					549:561	extensibility	549:561	extensibility	549:561	Rice hydrolysate improved the film solubility and extensibility.					
35537582	4	24	dep	film	529:532	arg1	solubility					534:543	solubility	534:543	solubility	534:543	Rice hydrolysate improved the film solubility and extensibility.					
35537582	2	25	theme	different	197:205	arg1	MW					235:236	MW	235:236	MW	235:236	Effect of four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa) was evaluated and curcumin was further incorporated into the film to enhance its bioactivities.					
35537582	2	25	theme	different	197:205	arg1	weights					226:232	four different chitosan molecular weights	192:232	four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa)	192:265	Effect of four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa) was evaluated and curcumin was further incorporated into the film to enhance its bioactivities.					
35537582	10	26	theme	protein	1059:1065	arg1	hydrolysate					1067:1077	protein hydrolysate	1059:1077	protein hydrolysate	1059:1077	Hence, chitosan molecular weight must be chosen carefully according to its application and incorporation of protein hydrolysate into film would be an efficient strategy to modify film performance.					
35537582	3	27	theme	water	419:423	arg1	vapor					425:429	water vapor	419:429	water vapor transmission	419:442	With increasing MW (<100 kDa), moisture, solubility and water vapor transmission marginally decreased while tensile strength increased.					
35537582	7	28	theme	higher	711:716	arg1	activity					730:737	the higher antioxidant activity	707:737	the higher antioxidant activity of the CH/RH films	707:756	The lower MW, the higher antioxidant activity of the CH/RH films.					
35537582	3	29	theme	vapor	425:429	arg1	transmission					431:442	water vapor transmission	419:442	water vapor transmission	419:442	With increasing MW (<100 kDa), moisture, solubility and water vapor transmission marginally decreased while tensile strength increased.					
35537582	8	30	theme	30 kDa	763:768	arg1	film					776:779	The 30 kDa CH/RH film	759:779	The 30 kDa CH/RH film	759:779	The 30 kDa CH/RH film exhibited higher antibacterial activity against both E. coli and S. aureus.					
35537582	7	31	theme	antioxidant	718:728	arg1	activity					730:737	the higher antioxidant activity	707:737	the higher antioxidant activity of the CH/RH films	707:756	The lower MW, the higher antioxidant activity of the CH/RH films.					
35537582	10	32	theme	chitosan	958:965	arg1	weight					977:982	chitosan molecular weight	958:982	chitosan molecular weight	958:982	Hence, chitosan molecular weight must be chosen carefully according to its application and incorporation of protein hydrolysate into film would be an efficient strategy to modify film performance.					
35537582	0	33	dep	film	45:48	arg1	Effect					51:56	Effect	51:56	Chitosan/rice hydrolysate/curcumin composite film: Effect of chitosan molecular weight.	0:86	Chitosan/rice hydrolysate/curcumin composite film: Effect of chitosan molecular weight.					
35537582	1	34	theme	rice	132:135	arg1	RH					158:159	RH	158:159	RH	158:159	The composite films were prepared by adding rice protein hydrolysate (RH) into chitosan (CH).					
35537582	1	34	theme	rice	132:135	arg1	hydrolysate					145:155	rice protein hydrolysate	132:155	rice protein hydrolysate (RH)	132:160	The composite films were prepared by adding rice protein hydrolysate (RH) into chitosan (CH).					
35537582	1	35	theme	protein	137:143	arg1	RH					158:159	RH	158:159	RH	158:159	The composite films were prepared by adding rice protein hydrolysate (RH) into chitosan (CH).					
35537582	1	35	theme	protein	137:143	arg1	hydrolysate					145:155	rice protein hydrolysate	132:155	rice protein hydrolysate (RH)	132:160	The composite films were prepared by adding rice protein hydrolysate (RH) into chitosan (CH).					
35537582	0	36	theme	weight	80:85	arg1	Effect					51:56	Effect	51:56	Chitosan/rice hydrolysate/curcumin composite film: Effect of chitosan molecular weight.	0:86	Chitosan/rice hydrolysate/curcumin composite film: Effect of chitosan molecular weight.					
35537582	8	37	theme	CH/RH	770:774	arg1	film					776:779	The 30 kDa CH/RH film	759:779	The 30 kDa CH/RH film	759:779	The 30 kDa CH/RH film exhibited higher antibacterial activity against both E. coli and S. aureus.					
35537582	7	38	theme	lower	697:701	arg1	MW					703:704	The lower MW	693:704	The lower MW	693:704	The lower MW, the higher antioxidant activity of the CH/RH films.					
35537582	0	39	theme	molecular	70:78	arg1	weight					80:85	chitosan molecular weight	61:85	chitosan molecular weight	61:85	Chitosan/rice hydrolysate/curcumin composite film: Effect of chitosan molecular weight.					
35537582	2	40	theme	weights	226:232	arg1	Effect					182:187	Effect	182:187	Effect of four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa)	182:265	Effect of four different chitosan molecular weights (MW, 0.8- 1.0, 30, 100, 300 kDa) was evaluated and curcumin was further incorporated into the film to enhance its bioactivities.					
35537582	8	41	theme	higher	791:796	arg1	activity					812:819	higher antibacterial activity	791:819	higher antibacterial activity	791:819	The 30 kDa CH/RH film exhibited higher antibacterial activity against both E. coli and S. aureus.					
35537582	10	42	theme	film	1130:1133	arg1	performance					1135:1145	film performance	1130:1145	film performance	1130:1145	Hence, chitosan molecular weight must be chosen carefully according to its application and incorporation of protein hydrolysate into film would be an efficient strategy to modify film performance.					
36398565	0	0	theme	angiogenic	97:106	arg1	property					108:115	angiogenic property	97:115	angiogenic property	97:115	Chitosan-placental ECM composite thermos-responsive hydrogel as a biomimetic wound dressing with angiogenic property.					
36398565	2	1	theme	smart	448:452	arg1	hydrogel					469:476	a smart wound dressing hydrogel	446:476	a smart wound dressing hydrogel with improved biological behavior	446:510	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	6	2	with	hydrogels	975:983	arg1	concentration					999:1011	a higher concentration	990:1011	a higher concentration of ECM	990:1018	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	1	3	theme	wound	162:166	arg1	dressing					168:175	an ideal wound dressing	153:175	an ideal wound dressing with excellent biomechanical and biological properties	153:230	Attempts are being made to develop an ideal wound dressing with excellent biomechanical and biological properties.					
36398565	8	4	theme	improved	1357:1364	arg1	properties					1377:1386	improved angiogenic properties	1357:1386	improved angiogenic properties	1357:1386	The data obtained from the current study suggest the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations.					
36398565	1	5	with	dressing	168:175	arg1	properties					221:230	excellent biomechanical and biological properties	182:230	excellent biomechanical and biological properties	182:230	Attempts are being made to develop an ideal wound dressing with excellent biomechanical and biological properties.					
36398565	3	6	theme	uniform	595:601	arg1	morphology					603:612	uniform morphology	595:612	uniform morphology	595:612	The thermo-responsive CTS (TCTS) alone or loaded with ECMs (ECM-TCTS) demonstrate uniform morphology using SEM.					
36398565	2	7	theme	biological	492:501	arg1	behavior					503:510	improved biological behavior	483:510	improved biological behavior	483:510	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	5	8	theme	ECM	851:853	arg1	content					855:861	the ECM content	847:861	the ECM content of the hydrogel increase	847:886	The degradation and swelling ratios increase as the ECM content of the hydrogel increase.					
36398565	6	9	theme	ECM	1016:1018	arg1	concentration					999:1011	a higher concentration	990:1011	a higher concentration of ECM	990:1018	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	2	10	theme	improved	483:490	arg1	behavior					503:510	improved biological behavior	483:510	improved biological behavior	483:510	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	4	11	theme	5 min	686:690	arg1	time					678:681	a gelation time	667:681	a gelation time of 5 min	667:690	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	0	12	with	dressing	83:90	arg1	property					108:115	angiogenic property	97:115	angiogenic property	97:115	Chitosan-placental ECM composite thermos-responsive hydrogel as a biomimetic wound dressing with angiogenic property.					
36398565	3	13	theme	thermo-responsive	517:533	arg1	TCTS					540:543	TCTS	540:543	TCTS	540:543	The thermo-responsive CTS (TCTS) alone or loaded with ECMs (ECM-TCTS) demonstrate uniform morphology using SEM.					
36398565	3	13	theme	thermo-responsive	517:533	arg1	CTS					535:537	The thermo-responsive CTS	513:537	The thermo-responsive CTS (TCTS) alone or loaded with ECMs (ECM-TCTS)	513:581	The thermo-responsive CTS (TCTS) alone or loaded with ECMs (ECM-TCTS) demonstrate uniform morphology using SEM.					
36398565	6	14	from	HUVEC	1140:1144	arg1	expression					1085:1094	expression	1085:1094	expression of angiogenic factors (VEGF and VEGFR) from HUVEC	1085:1144	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	4	15	theme	-TCTS	639:643	arg1	-TCTS					656:660	TCTS and ECM1%-TCTS and ECM2.5%-TCTS	625:660	TCTS and ECM1%-TCTS and ECM2.5%-TCTS	625:660	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	4	16	theme	25°C.	748:752	arg1	-TCTS					759:763	25°C. ECM5%-TCTS	748:763	25°C. ECM5%-TCTS	748:763	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	8	17	theme	smart	1320:1324	arg1	dressing					1343:1350	an optimized smart biomimetic wound dressing	1307:1350	an optimized smart biomimetic wound dressing with improved angiogenic properties	1307:1386	The data obtained from the current study suggest the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations.					
36398565	7	18	theme	antibacterial	1171:1183	arg1	activity					1185:1192	antibacterial activity	1171:1192	antibacterial activity against Acinetobacter baumannii ATCC 19606	1171:1235	Only the ECM5%-TCTS has antibacterial activity against Acinetobacter baumannii ATCC 19606.					
36398565	2	19	theme	solubilized	358:368	arg1	ECM					402:404	ECM	402:404	ECM	402:404	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	2	19	theme	solubilized	358:368	arg1	matrix					394:399	solubilized placental extracellular matrix	358:399	solubilized placental extracellular matrix (ECM)	358:405	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	6	20	theme	higher	992:997	arg1	concentration					999:1011	a higher concentration	990:1011	a higher concentration of ECM	990:1018	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	5	21	theme	swelling	819:826	arg1	ratios					828:833	The degradation and swelling ratios	799:833	The degradation and swelling ratios	799:833	The degradation and swelling ratios increase as the ECM content of the hydrogel increase.					
36398565	8	22	theme	biomimetic	1326:1335	arg1	dressing					1343:1350	an optimized smart biomimetic wound dressing	1307:1350	an optimized smart biomimetic wound dressing with improved angiogenic properties	1307:1386	The data obtained from the current study suggest the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations.					
36398565	1	23	theme	excellent	182:190	arg1	properties					221:230	excellent biomechanical and biological properties	182:230	excellent biomechanical and biological properties	182:230	Attempts are being made to develop an ideal wound dressing with excellent biomechanical and biological properties.					
36398565	2	24	theme	20	411:412	arg1	%					413:413	%	413:413	%	413:413	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	6	25	theme	cell	1039:1042	arg1	adhesion					1044:1051	better cell adhesion	1032:1051	better cell adhesion for fibroblast cells	1032:1072	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	4	26	theme	%	758:758	arg1	-TCTS					759:763	25°C. ECM5%-TCTS	748:763	25°C. ECM5%-TCTS	748:763	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	2	27	with	hydrogel	469:476	arg1	behavior					503:510	improved biological behavior	483:510	improved biological behavior	483:510	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	4	28	theme	gelation	669:676	arg1	time					678:681	a gelation time	667:681	a gelation time of 5 min	667:690	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	1	29	theme	biomechanical	192:204	arg1	properties					221:230	excellent biomechanical and biological properties	182:230	excellent biomechanical and biological properties	182:230	Attempts are being made to develop an ideal wound dressing with excellent biomechanical and biological properties.					
36398565	0	30	theme	Chitosan-placental	0:17	arg1	hydrogel					52:59	Chitosan-placental ECM composite thermos-responsive hydrogel	0:59	Chitosan-placental ECM composite thermos-responsive hydrogel	0:59	Chitosan-placental ECM composite thermos-responsive hydrogel as a biomimetic wound dressing with angiogenic property.					
36398565	4	31	theme	ECM5	754:757	arg1	-TCTS					759:763	25°C. ECM5%-TCTS	748:763	25°C. ECM5%-TCTS	748:763	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	6	32	theme	better	1032:1037	arg1	adhesion					1044:1051	better cell adhesion	1032:1051	better cell adhesion for fibroblast cells	1032:1072	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	4	33	theme	TCTS	625:628	arg1	-TCTS					656:660	TCTS and ECM1%-TCTS and ECM2.5%-TCTS	625:660	TCTS and ECM1%-TCTS and ECM2.5%-TCTS	625:660	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	0	34	theme	thermos-responsive	33:50	arg1	hydrogel					52:59	Chitosan-placental ECM composite thermos-responsive hydrogel	0:59	Chitosan-placental ECM composite thermos-responsive hydrogel	0:59	Chitosan-placental ECM composite thermos-responsive hydrogel as a biomimetic wound dressing with angiogenic property.					
36398565	8	35	theme	pre-clinical	1417:1428	arg1	investigations					1443:1456	pre-clinical and clinical investigations	1417:1456	pre-clinical and clinical investigations	1417:1456	The data obtained from the current study suggest the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations.					
36398565	8	36	theme	optimized	1310:1318	arg1	dressing					1343:1350	an optimized smart biomimetic wound dressing	1307:1350	an optimized smart biomimetic wound dressing with improved angiogenic properties	1307:1386	The data obtained from the current study suggest the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations.					
36398565	6	37	theme	angiogenic	1099:1108	arg1	factors					1110:1116	angiogenic factors	1099:1116	angiogenic factors (VEGF and VEGFR)	1099:1133	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	6	37	theme	angiogenic	1099:1108	arg1	VEGFR					1128:1132	VEGFR	1128:1132	VEGFR	1128:1132	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	6	37	theme	angiogenic	1099:1108	arg1	VEGF					1119:1122	VEGF	1119:1122	VEGF	1119:1122	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	6	38	dep	factors	1110:1116	arg1	factors					1110:1116	angiogenic factors	1099:1116	angiogenic factors (VEGF and VEGFR)	1099:1133	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	6	38	dep	factors	1110:1116	arg1	VEGFR					1128:1132	VEGFR	1128:1132	VEGFR	1128:1132	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	6	38	dep	factors	1110:1116	arg1	VEGF					1119:1122	VEGF	1119:1122	VEGF	1119:1122	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	1	39	theme	biological	210:219	arg1	properties					221:230	excellent biomechanical and biological properties	182:230	excellent biomechanical and biological properties	182:230	Attempts are being made to develop an ideal wound dressing with excellent biomechanical and biological properties.					
36398565	0	40	theme	composite	23:31	arg1	hydrogel					52:59	Chitosan-placental ECM composite thermos-responsive hydrogel	0:59	Chitosan-placental ECM composite thermos-responsive hydrogel	0:59	Chitosan-placental ECM composite thermos-responsive hydrogel as a biomimetic wound dressing with angiogenic property.					
36398565	2	41	theme	various	309:315	arg1	concentrations					317:330	various concentrations	309:330	various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM)	309:405	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	2	42	theme	matrix	394:399	arg1	concentrations					317:330	various concentrations	309:330	various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM)	309:405	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	8	43	theme	clinical	1434:1441	arg1	investigations					1443:1456	pre-clinical and clinical investigations	1417:1456	pre-clinical and clinical investigations	1417:1456	The data obtained from the current study suggest the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations.					
36398565	7	44	theme	ECM5	1156:1159	arg1	-TCTS					1161:1165	Only the ECM5%-TCTS	1147:1165	Only the ECM5%-TCTS	1147:1165	Only the ECM5%-TCTS has antibacterial activity against Acinetobacter baumannii ATCC 19606.					
36398565	8	45	theme	current	1265:1271	arg1	study					1273:1277	the current study	1261:1277	the current study	1261:1277	The data obtained from the current study suggest the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations.					
36398565	2	46	theme	extracellular	380:392	arg1	ECM					402:404	ECM	402:404	ECM	402:404	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	2	46	theme	extracellular	380:392	arg1	matrix					394:399	solubilized placental extracellular matrix	358:399	solubilized placental extracellular matrix (ECM)	358:405	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	4	47	theme	%	638:638	arg1	-TCTS					639:643	ECM1%-TCTS and ECM2.5%	634:655	-TCTS	639:643	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	6	48	theme	fibroblast	1057:1066	arg1	cells					1068:1072	fibroblast cells	1057:1072	fibroblast cells	1057:1072	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	2	49	theme	thermos-responsive	241:258	arg1	hydrogel					260:267	a thermos-responsive hydrogel	239:267	a thermos-responsive hydrogel	239:267	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	2	50	theme	placental	370:378	arg1	ECM					402:404	ECM	402:404	ECM	402:404	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	2	50	theme	placental	370:378	arg1	matrix					394:399	solubilized placental extracellular matrix	358:399	solubilized placental extracellular matrix (ECM)	358:405	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	0	51	theme	biomimetic	66:75	arg1	dressing					83:90	a biomimetic wound dressing	64:90	a biomimetic wound dressing with angiogenic property	64:115	Chitosan-placental ECM composite thermos-responsive hydrogel as a biomimetic wound dressing with angiogenic property.					
36398565	0	52	theme	wound	77:81	arg1	dressing					83:90	a biomimetic wound dressing	64:90	a biomimetic wound dressing with angiogenic property	64:115	Chitosan-placental ECM composite thermos-responsive hydrogel as a biomimetic wound dressing with angiogenic property.					
36398565	6	53	theme	factors	1110:1116	arg1	expression					1085:1094	expression	1085:1094	expression of angiogenic factors (VEGF and VEGFR) from HUVEC	1085:1144	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	2	54	dep	concentrations	317:330	arg1	%					334:334	1%	333:334	1%	333:334	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	2	54	dep	concentrations	317:330	arg1	%					340:340	2.5%	337:340	2.5%	337:340	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	2	54	dep	concentrations	317:330	arg1	w/v					350:352	5% w/v	347:352	5% w/v	347:352	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	2	55	theme	%	413:413	arg1	β-glycerophosphate					415:432	20% β-glycerophosphate	411:432	20% β-glycerophosphate	411:432	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	2	56	with	chitosan	289:296	arg1	concentrations					317:330	various concentrations	309:330	various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM)	309:405	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	8	57	theme	wound	1337:1341	arg1	dressing					1343:1350	an optimized smart biomimetic wound dressing	1307:1350	an optimized smart biomimetic wound dressing with improved angiogenic properties	1307:1386	The data obtained from the current study suggest the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations.					
36398565	0	58	dep	hydrogel	52:59	arg1	dressing					83:90	a biomimetic wound dressing	64:90	a biomimetic wound dressing with angiogenic property	64:115	Chitosan-placental ECM composite thermos-responsive hydrogel as a biomimetic wound dressing with angiogenic property.					
36398565	7	59	contain	has	1167:1169	arg1	-TCTS					1161:1165	Only the ECM5%-TCTS	1147:1165	Only the ECM5%-TCTS	1147:1165	Only the ECM5%-TCTS has antibacterial activity against Acinetobacter baumannii ATCC 19606.					
36398565	7	59	contain	has	1167:1169	arg2	activity					1185:1192	antibacterial activity	1171:1192	antibacterial activity against Acinetobacter baumannii ATCC 19606	1171:1235	Only the ECM5%-TCTS has antibacterial activity against Acinetobacter baumannii ATCC 19606.					
36398565	8	60	theme	%	1297:1297	arg1	-TCTS					1298:1302	the ECM2.5%-TCTS	1287:1302	the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations	1287:1456	The data obtained from the current study suggest the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations.					
36398565	8	61	theme	ECM2.5	1291:1296	arg1	%					1297:1297	%	1297:1297	%	1297:1297	The data obtained from the current study suggest the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations.					
36398565	5	62	theme	hydrogel	870:877	arg1	increase					879:886	the hydrogel increase	866:886	the hydrogel increase	866:886	The degradation and swelling ratios increase as the ECM content of the hydrogel increase.					
36398565	4	63	theme	ECM1	634:637	arg1	-TCTS					639:643	ECM1%-TCTS and ECM2.5%	634:655	-TCTS	639:643	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	5	64	theme	degradation	803:813	arg1	ratios					828:833	The degradation and swelling ratios	799:833	The degradation and swelling ratios	799:833	The degradation and swelling ratios increase as the ECM content of the hydrogel increase.					
36398565	8	65	with	dressing	1343:1350	arg1	properties					1377:1386	improved angiogenic properties	1357:1386	improved angiogenic properties	1357:1386	The data obtained from the current study suggest the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations.					
36398565	6	66	theme	excellent	913:921	arg1	biocompatibility					923:938	excellent biocompatibility	913:938	excellent biocompatibility	913:938	All the constructs show excellent biocompatibility in vitro and in vivo, however, the hydrogels with a higher concentration of ECM demonstrate better cell adhesion for fibroblast cells and induce expression of angiogenic factors (VEGF and VEGFR) from HUVEC.					
36398565	5	67	theme	increase	879:886	arg1	content					855:861	the ECM content	847:861	the ECM content of the hydrogel increase	847:886	The degradation and swelling ratios increase as the ECM content of the hydrogel increase.					
36398565	2	68	with	β-glycerophosphate	415:432	arg1	concentrations					317:330	various concentrations	309:330	various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM)	309:405	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	1	69	theme	ideal	156:160	arg1	dressing					168:175	an ideal wound dressing	153:175	an ideal wound dressing with excellent biomechanical and biological properties	153:230	Attempts are being made to develop an ideal wound dressing with excellent biomechanical and biological properties.					
36398565	4	70	located	observed	728:735	arg1	gel					772:774	gel	772:774	gel	772:774	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	4	70	located	observed	728:735	arg2	formation					714:722	no gel formation	707:722	no gel formation	707:722	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	4	70	located	observed	728:735	arg1	-TCTS					759:763	25°C. ECM5%-TCTS	748:763	25°C. ECM5%-TCTS	748:763	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	4	70	located	observed	728:735	arg1	4°C					740:742	4°C	740:742	4°C	740:742	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	2	71	theme	dressing	460:467	arg1	hydrogel					469:476	a smart wound dressing hydrogel	446:476	a smart wound dressing hydrogel with improved biological behavior	446:510	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	7	72	theme	%	1160:1160	arg1	-TCTS					1161:1165	Only the ECM5%-TCTS	1147:1165	Only the ECM5%-TCTS	1147:1165	Only the ECM5%-TCTS has antibacterial activity against Acinetobacter baumannii ATCC 19606.					
36398565	7	73	theme	baumannii	1216:1224	arg1	ATCC					1226:1229	Acinetobacter baumannii ATCC 19606	1202:1235	Acinetobacter baumannii ATCC 19606	1202:1235	Only the ECM5%-TCTS has antibacterial activity against Acinetobacter baumannii ATCC 19606.					
36398565	2	74	theme	%	348:348	arg1	w/v					350:352	5% w/v	347:352	5% w/v	347:352	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	7	75	theme	Acinetobacter	1202:1214	arg1	ATCC					1226:1229	Acinetobacter baumannii ATCC 19606	1202:1235	Acinetobacter baumannii ATCC 19606	1202:1235	Only the ECM5%-TCTS has antibacterial activity against Acinetobacter baumannii ATCC 19606.					
36398565	2	76	theme	wound	454:458	arg1	hydrogel					469:476	a smart wound dressing hydrogel	446:476	a smart wound dressing hydrogel with improved biological behavior	446:510	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	8	77	theme	angiogenic	1366:1375	arg1	properties					1377:1386	improved angiogenic properties	1357:1386	improved angiogenic properties	1357:1386	The data obtained from the current study suggest the ECM2.5%-TCTS as an optimized smart biomimetic wound dressing with improved angiogenic properties now promises to proceed with pre-clinical and clinical investigations.					
36398565	4	78	theme	gel	710:712	arg1	formation					714:722	no gel formation	707:722	no gel formation	707:722	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	4	79	dep	formed	765:770	arg1	gel					772:774	gel	772:774	gel	772:774	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	4	79	dep	formed	765:770	arg1	4°C					740:742	4°C	740:742	4°C	740:742	TCTS and ECM1%-TCTS and ECM2.5%-TCTS show a gelation time of 5 min at 37°C, while no gel formation was observed at 4°C and 25°C. ECM5%-TCTS formed gel at both 25°C and 37°C.					
36398565	2	80	theme	5	347:347	arg1	%					348:348	%	348:348	%	348:348	Here, a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1%, 2.5%, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogel with improved biological behavior.					
36398565	0	81	theme	ECM	19:21	arg1	hydrogel					52:59	Chitosan-placental ECM composite thermos-responsive hydrogel	0:59	Chitosan-placental ECM composite thermos-responsive hydrogel	0:59	Chitosan-placental ECM composite thermos-responsive hydrogel as a biomimetic wound dressing with angiogenic property.					
37200222	7	0	theme	pH	1301:1302	arg1	levels					1304:1309	lower pH levels	1295:1309	lower pH levels	1295:1309	The swelling and bioresorbability ratios of these hydrogels are favorably adjusted at lower pH levels, which correspond to the ones in inflamed tissues.					
37200222	9	1	theme	AuNPs	1547:1551	arg1	presence					1535:1542	the presence	1531:1542	the presence of AuNPs	1531:1551	Additionally, the presence of AuNPs allowed the hydrogels to be monitorable through computer tomography.					
37200222	4	2	theme	proteic	629:635	arg1	nanostructures					637:650	proteic nanostructures	629:650	proteic nanostructures	629:650	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	4	2	theme	proteic	629:635	arg1	nanofibrils					605:615	lysozyme nanofibrils	596:615	lysozyme nanofibrils (LNFs)	596:622	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	4	2	theme	proteic	629:635	arg1	nanofibrils					569:579	Protein nanofibrils	561:579	Protein nanofibrils (NFs)	561:585	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	0	3	theme	Nanofibrils-Gold	94:109	arg1	Nanoparticles					111:123	Hybrid Lysozyme Nanofibrils-Gold Nanoparticles	78:123	Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium	78:166	Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid Reinforced with Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium.					
37200222	1	4	theme	promising	207:215	arg1	hydrogels					193:201	Biopolymeric injectable hydrogels	169:201	Biopolymeric injectable hydrogels	169:201	Biopolymeric injectable hydrogels are promising biomaterials for myocardial regeneration applications.					
37200222	1	4	theme	promising	207:215	arg1	biomaterials					217:228	promising biomaterials	207:228	promising biomaterials for myocardial regeneration applications	207:269	Biopolymeric injectable hydrogels are promising biomaterials for myocardial regeneration applications.					
37200222	7	5	theme	inflamed	1344:1351	arg1	tissues					1353:1359	inflamed tissues	1344:1359	inflamed tissues	1344:1359	The swelling and bioresorbability ratios of these hydrogels are favorably adjusted at lower pH levels, which correspond to the ones in inflamed tissues.					
37200222	6	6	theme	antioxidant	1106:1116	arg1	activity					1118:1125	antioxidant activity	1106:1125	antioxidant activity	1106:1125	The resulting nanocomposite hydrogels showed improved rheological properties, mechanical resilience, antioxidant activity, and electrical conductivity, especially for the hydrogels containing AuNPs@LNFs.					
37200222	4	7	dep	such	588:591	arg1	as					593:594	as	593:594	as	593:594	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	10	8	theme	@	1664:1664	arg1	LNFs					1665:1668	AuNPs@LNFs	1659:1668	AuNPs@LNFs	1659:1668	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	10	8	theme	@	1664:1664	arg1	LNFs					1650:1653	LNFs	1650:1653	LNFs	1650:1653	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	10	8	theme	@	1664:1664	arg1	nanostructures					1695:1708	excellent functional nanostructures	1674:1708	excellent functional nanostructures	1674:1708	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	6	9	theme	rheological	1059:1069	arg1	properties					1071:1080	improved rheological properties	1050:1080	improved rheological properties	1050:1080	The resulting nanocomposite hydrogels showed improved rheological properties, mechanical resilience, antioxidant activity, and electrical conductivity, especially for the hydrogels containing AuNPs@LNFs.					
37200222	3	10	theme	desirable	433:441	arg1	activity					480:487	antioxidant activity	468:487	antioxidant activity	468:487	However, due to their nature, biopolymeric hydrogels usually lack desirable functionalities, such as antioxidant activity and electrical conductivity, and in some cases, mechanical performance.					
37200222	3	10	theme	desirable	433:441	arg1	functionalities					443:457	desirable functionalities	433:457	desirable functionalities	433:457	However, due to their nature, biopolymeric hydrogels usually lack desirable functionalities, such as antioxidant activity and electrical conductivity, and in some cases, mechanical performance.					
37200222	3	10	theme	desirable	433:441	arg1	conductivity					504:515	electrical conductivity	493:515	electrical conductivity	493:515	However, due to their nature, biopolymeric hydrogels usually lack desirable functionalities, such as antioxidant activity and electrical conductivity, and in some cases, mechanical performance.					
37200222	7	11	theme	bioresorbability	1226:1241	arg1	ratios					1243:1248	The swelling and bioresorbability ratios	1209:1248	The swelling and bioresorbability ratios of these hydrogels	1209:1267	The swelling and bioresorbability ratios of these hydrogels are favorably adjusted at lower pH levels, which correspond to the ones in inflamed tissues.					
37200222	5	12	theme	myocardial	967:976	arg1	applications					991:1002	myocardial regeneration applications	967:1002	myocardial regeneration applications	967:1002	Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications.					
37200222	5	13	theme	hybrid	883:888	arg1	LNFs					896:899	the obtained hybrid AuNPs@LNFs	870:899	the obtained hybrid AuNPs@LNFs	870:899	Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications.					
37200222	4	14	theme	antioxidant	694:704	arg1	activity					706:713	antioxidant activity	694:713	antioxidant activity	694:713	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	2	15	theme	surrounding	347:357	arg1	tissue					359:364	the surrounding tissue	343:364	the surrounding tissue	343:364	Besides being biocompatible, they adjust themselves, perfectly fitting the surrounding tissue.					
37200222	10	16	theme	nanocomposite	1747:1759	arg1	hydrogels					1761:1769	injectable biopolymeric nanocomposite hydrogels	1723:1769	injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications	1723:1810	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	6	17	theme	improved	1050:1057	arg1	properties					1071:1080	improved rheological properties	1050:1080	improved rheological properties	1050:1080	The resulting nanocomposite hydrogels showed improved rheological properties, mechanical resilience, antioxidant activity, and electrical conductivity, especially for the hydrogels containing AuNPs@LNFs.					
37200222	5	18	theme	LNFs	860:863	arg1	presence					848:855	the presence	844:855	the presence of LNFs	844:863	Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications.					
37200222	7	19	theme	swelling	1213:1220	arg1	ratios					1243:1248	The swelling and bioresorbability ratios	1209:1248	The swelling and bioresorbability ratios of these hydrogels	1209:1267	The swelling and bioresorbability ratios of these hydrogels are favorably adjusted at lower pH levels, which correspond to the ones in inflamed tissues.					
37200222	10	20	theme	excellent	1674:1682	arg1	LNFs					1665:1668	AuNPs@LNFs	1659:1668	AuNPs@LNFs	1659:1668	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	10	20	theme	excellent	1674:1682	arg1	LNFs					1650:1653	LNFs	1650:1653	LNFs	1650:1653	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	10	20	theme	excellent	1674:1682	arg1	nanostructures					1695:1708	excellent functional nanostructures	1674:1708	excellent functional nanostructures	1674:1708	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	0	21	theme	Nanocomposite	11:23	arg1	Hydrogels					25:33	Injectable Nanocomposite Hydrogels	0:33	Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid	0:60	Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid Reinforced with Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium.					
37200222	4	22	theme	Protein	561:567	arg1	nanostructures					637:650	proteic nanostructures	629:650	proteic nanostructures	629:650	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	4	22	theme	Protein	561:567	arg1	NFs					582:584	NFs	582:584	NFs	582:584	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	4	22	theme	Protein	561:567	arg1	nanofibrils					569:579	Protein nanofibrils	561:579	Protein nanofibrils (NFs)	561:585	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	4	22	theme	Protein	561:567	arg1	nanofibrils					605:615	lysozyme nanofibrils	596:615	lysozyme nanofibrils (LNFs)	596:622	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	10	23	theme	AuNPs	1659:1663	arg1	LNFs					1665:1668	AuNPs@LNFs	1659:1668	AuNPs@LNFs	1659:1668	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	10	23	theme	AuNPs	1659:1663	arg1	LNFs					1650:1653	LNFs	1650:1653	LNFs	1650:1653	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	10	23	theme	AuNPs	1659:1663	arg1	nanostructures					1695:1708	excellent functional nanostructures	1674:1708	excellent functional nanostructures	1674:1708	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	5	24	theme	regeneration	978:989	arg1	applications					991:1002	myocardial regeneration applications	967:1002	myocardial regeneration applications	967:1002	Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications.					
37200222	7	25	theme	hydrogels	1259:1267	arg1	ratios					1243:1248	The swelling and bioresorbability ratios	1209:1248	The swelling and bioresorbability ratios of these hydrogels	1209:1267	The swelling and bioresorbability ratios of these hydrogels are favorably adjusted at lower pH levels, which correspond to the ones in inflamed tissues.					
37200222	1	26	theme	myocardial	234:243	arg1	applications					258:269	myocardial regeneration applications	234:269	myocardial regeneration applications	234:269	Biopolymeric injectable hydrogels are promising biomaterials for myocardial regeneration applications.					
37200222	0	27	theme	Injectable	0:9	arg1	Hydrogels					25:33	Injectable Nanocomposite Hydrogels	0:33	Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid	0:60	Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid Reinforced with Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium.					
37200222	1	28	theme	regeneration	245:256	arg1	applications					258:269	myocardial regeneration applications	234:269	myocardial regeneration applications	234:269	Biopolymeric injectable hydrogels are promising biomaterials for myocardial regeneration applications.					
37200222	0	29	theme	Myocardium	157:166	arg1	Regeneration					133:144	the Regeneration	129:144	the Regeneration of Damaged Myocardium	129:166	Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid Reinforced with Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium.					
37200222	7	30	from	ones	1336:1339	arg1	tissues					1353:1359	inflamed tissues	1344:1359	inflamed tissues	1344:1359	The swelling and bioresorbability ratios of these hydrogels are favorably adjusted at lower pH levels, which correspond to the ones in inflamed tissues.					
37200222	8	31	theme	important	1413:1421	arg1	properties					1423:1432	important properties	1413:1432	important properties	1413:1432	These improvements were observed while maintaining important properties, namely, injectability, biocompatibility, and the ability to release a model drug.					
37200222	5	32	theme	obtained	874:881	arg1	LNFs					896:899	the obtained hybrid AuNPs@LNFs	870:899	the obtained hybrid AuNPs@LNFs	870:899	Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications.					
37200222	6	33	theme	@	1202:1202	arg1	LNFs					1203:1206	AuNPs@LNFs	1197:1206	AuNPs@LNFs	1197:1206	The resulting nanocomposite hydrogels showed improved rheological properties, mechanical resilience, antioxidant activity, and electrical conductivity, especially for the hydrogels containing AuNPs@LNFs.					
37200222	6	34	theme	nanocomposite	1019:1031	arg1	hydrogels					1033:1041	The resulting nanocomposite hydrogels	1005:1041	The resulting nanocomposite hydrogels	1005:1041	The resulting nanocomposite hydrogels showed improved rheological properties, mechanical resilience, antioxidant activity, and electrical conductivity, especially for the hydrogels containing AuNPs@LNFs.					
37200222	0	35	theme	Damaged	149:155	arg1	Myocardium					157:166	Damaged Myocardium	149:166	Damaged Myocardium	149:166	Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid Reinforced with Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium.					
37200222	4	36	theme	metallic	759:766	arg1	nanoparticles					768:780	metallic nanoparticles	759:780	metallic nanoparticles	759:780	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	3	37	theme	mechanical	537:546	arg1	performance					548:558	mechanical performance	537:558	mechanical performance	537:558	However, due to their nature, biopolymeric hydrogels usually lack desirable functionalities, such as antioxidant activity and electrical conductivity, and in some cases, mechanical performance.					
37200222	0	38	theme	Acid	57:60	arg1	Hydrogels					25:33	Injectable Nanocomposite Hydrogels	0:33	Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid	0:60	Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid Reinforced with Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium.					
37200222	6	39	theme	resulting	1009:1017	arg1	hydrogels					1033:1041	The resulting nanocomposite hydrogels	1005:1041	The resulting nanocomposite hydrogels	1005:1041	The resulting nanocomposite hydrogels showed improved rheological properties, mechanical resilience, antioxidant activity, and electrical conductivity, especially for the hydrogels containing AuNPs@LNFs.					
37200222	10	40	theme	regeneration	1786:1797	arg1	applications					1799:1810	myocardial regeneration applications	1775:1810	myocardial regeneration applications	1775:1810	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	10	41	theme	biopolymeric	1734:1745	arg1	hydrogels					1761:1769	injectable biopolymeric nanocomposite hydrogels	1723:1769	injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications	1723:1810	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	5	42	theme	AuNPs	890:894	arg1	LNFs					896:899	the obtained hybrid AuNPs@LNFs	870:899	the obtained hybrid AuNPs@LNFs	870:899	Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications.					
37200222	3	43	theme	electrical	493:502	arg1	conductivity					504:515	electrical conductivity	493:515	electrical conductivity	493:515	However, due to their nature, biopolymeric hydrogels usually lack desirable functionalities, such as antioxidant activity and electrical conductivity, and in some cases, mechanical performance.					
37200222	0	44	theme	Gelatin-Hyaluronic	38:55	arg1	Acid					57:60	Gelatin-Hyaluronic Acid	38:60	Gelatin-Hyaluronic Acid	38:60	Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid Reinforced with Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium.					
37200222	5	45	theme	@	895:895	arg1	LNFs					896:899	the obtained hybrid AuNPs@LNFs	870:899	the obtained hybrid AuNPs@LNFs	870:899	Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications.					
37200222	3	46	theme	antioxidant	468:478	arg1	activity					480:487	antioxidant activity	468:487	antioxidant activity	468:487	However, due to their nature, biopolymeric hydrogels usually lack desirable functionalities, such as antioxidant activity and electrical conductivity, and in some cases, mechanical performance.					
37200222	5	47	theme	gelatin-hyaluronic	924:941	arg1	HA					949:950	HA	949:950	HA	949:950	Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications.					
37200222	5	47	theme	gelatin-hyaluronic	924:941	arg1	acid					943:946	gelatin-hyaluronic acid	924:946	gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications	924:1002	Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications.					
37200222	3	48	theme	biopolymeric	397:408	arg1	hydrogels					410:418	biopolymeric hydrogels	397:418	biopolymeric hydrogels	397:418	However, due to their nature, biopolymeric hydrogels usually lack desirable functionalities, such as antioxidant activity and electrical conductivity, and in some cases, mechanical performance.					
37200222	6	49	theme	AuNPs	1197:1201	arg1	LNFs					1203:1206	AuNPs@LNFs	1197:1206	AuNPs@LNFs	1197:1206	The resulting nanocomposite hydrogels showed improved rheological properties, mechanical resilience, antioxidant activity, and electrical conductivity, especially for the hydrogels containing AuNPs@LNFs.					
37200222	5	50	theme	gold	789:792	arg1	AuNPs					809:813	AuNPs	809:813	AuNPs	809:813	Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications.					
37200222	5	50	theme	gold	789:792	arg1	nanoparticles					794:806	gold nanoparticles	789:806	gold nanoparticles (AuNPs)	789:814	Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications.					
37200222	7	51	theme	lower	1295:1299	arg1	levels					1304:1309	lower pH levels	1295:1309	lower pH levels	1295:1309	The swelling and bioresorbability ratios of these hydrogels are favorably adjusted at lower pH levels, which correspond to the ones in inflamed tissues.					
37200222	4	52	theme	lysozyme	596:603	arg1	nanostructures					637:650	proteic nanostructures	629:650	proteic nanostructures	629:650	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	4	52	theme	lysozyme	596:603	arg1	LNFs					618:621	LNFs	618:621	LNFs	618:621	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	4	52	theme	lysozyme	596:603	arg1	nanofibrils					605:615	lysozyme nanofibrils	596:615	lysozyme nanofibrils (LNFs)	596:622	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	4	52	theme	lysozyme	596:603	arg1	nanofibrils					569:579	Protein nanofibrils	561:579	Protein nanofibrils (NFs)	561:585	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	10	53	theme	injectable	1723:1732	arg1	hydrogels					1761:1769	injectable biopolymeric nanocomposite hydrogels	1723:1769	injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications	1723:1810	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	8	54	theme	model	1505:1509	arg1	drug					1511:1514	a model drug	1503:1514	a model drug	1503:1514	These improvements were observed while maintaining important properties, namely, injectability, biocompatibility, and the ability to release a model drug.					
37200222	9	55	theme	computer	1601:1608	arg1	tomography					1610:1619	computer tomography	1601:1619	computer tomography	1601:1619	Additionally, the presence of AuNPs allowed the hydrogels to be monitorable through computer tomography.					
37200222	4	56	theme	mechanical	667:676	arg1	performance					678:688	excellent mechanical performance	657:688	excellent mechanical performance	657:688	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	6	57	contain	containing	1186:1195	arg2	LNFs					1203:1206	AuNPs@LNFs	1197:1206	AuNPs@LNFs	1197:1206	The resulting nanocomposite hydrogels showed improved rheological properties, mechanical resilience, antioxidant activity, and electrical conductivity, especially for the hydrogels containing AuNPs@LNFs.					
37200222	6	57	contain	containing	1186:1195	arg1	hydrogels					1176:1184	the hydrogels	1172:1184	the hydrogels containing AuNPs@LNFs	1172:1206	The resulting nanocomposite hydrogels showed improved rheological properties, mechanical resilience, antioxidant activity, and electrical conductivity, especially for the hydrogels containing AuNPs@LNFs.					
37200222	4	58	with	nanostructures	637:650	arg1	performance					678:688	excellent mechanical performance	657:688	excellent mechanical performance	657:688	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	4	58	with	nanostructures	637:650	arg1	activity					706:713	antioxidant activity	694:713	antioxidant activity	694:713	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	1	59	theme	Biopolymeric	169:180	arg1	hydrogels					193:201	Biopolymeric injectable hydrogels	169:201	Biopolymeric injectable hydrogels	169:201	Biopolymeric injectable hydrogels are promising biomaterials for myocardial regeneration applications.					
37200222	1	59	theme	Biopolymeric	169:180	arg1	biomaterials					217:228	promising biomaterials	207:228	promising biomaterials for myocardial regeneration applications	207:269	Biopolymeric injectable hydrogels are promising biomaterials for myocardial regeneration applications.					
37200222	4	60	theme	excellent	657:665	arg1	performance					678:688	excellent mechanical performance	657:688	excellent mechanical performance	657:688	Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles.					
37200222	0	61	theme	Lysozyme	85:92	arg1	Nanoparticles					111:123	Hybrid Lysozyme Nanofibrils-Gold Nanoparticles	78:123	Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium	78:166	Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid Reinforced with Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium.					
37200222	10	62	theme	myocardial	1775:1784	arg1	applications					1799:1810	myocardial regeneration applications	1775:1810	myocardial regeneration applications	1775:1810	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	6	63	theme	electrical	1132:1141	arg1	conductivity					1143:1154	electrical conductivity	1132:1154	electrical conductivity	1132:1154	The resulting nanocomposite hydrogels showed improved rheological properties, mechanical resilience, antioxidant activity, and electrical conductivity, especially for the hydrogels containing AuNPs@LNFs.					
37200222	1	64	theme	injectable	182:191	arg1	hydrogels					193:201	Biopolymeric injectable hydrogels	169:201	Biopolymeric injectable hydrogels	169:201	Biopolymeric injectable hydrogels are promising biomaterials for myocardial regeneration applications.					
37200222	1	64	theme	injectable	182:191	arg1	biomaterials					217:228	promising biomaterials	207:228	promising biomaterials for myocardial regeneration applications	207:269	Biopolymeric injectable hydrogels are promising biomaterials for myocardial regeneration applications.					
37200222	0	65	theme	Hybrid	78:83	arg1	Nanoparticles					111:123	Hybrid Lysozyme Nanofibrils-Gold Nanoparticles	78:123	Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium	78:166	Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid Reinforced with Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium.					
37200222	10	66	theme	functional	1684:1693	arg1	LNFs					1665:1668	AuNPs@LNFs	1659:1668	AuNPs@LNFs	1659:1668	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	10	66	theme	functional	1684:1693	arg1	LNFs					1650:1653	LNFs	1650:1653	LNFs	1650:1653	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	10	66	theme	functional	1684:1693	arg1	nanostructures					1695:1708	excellent functional nanostructures	1674:1708	excellent functional nanostructures	1674:1708	This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.					
37200222	5	67	theme	acid	943:946	arg1	hydrogels					953:961	gelatin-hyaluronic acid (HA) hydrogels	924:961	gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications	924:1002	Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications.					
37200222	6	68	theme	mechanical	1083:1092	arg1	resilience					1094:1103	mechanical resilience	1083:1103	mechanical resilience	1083:1103	The resulting nanocomposite hydrogels showed improved rheological properties, mechanical resilience, antioxidant activity, and electrical conductivity, especially for the hydrogels containing AuNPs@LNFs.					
36374979	4	0	theme	highest	930:936	arg1	content					948:954	the highest β-glucans content	926:954	the highest β-glucans content	926:954	Treatments 4 (with shells, rice bran, and manioc flour) and 6 (with shells, sorghum flour, and manioc flour) presented the highest β-glucans content.					
36374979	2	1	theme	sorghum	592:598	arg1	supplements					550:560	supplements	550:560	supplements (rice bran, manioc flour, and sorghum flour)	550:605	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	2	1	theme	sorghum	592:598	arg1	flour					600:604	sorghum flour	592:604	sorghum flour	592:604	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	8	2	theme	dietary	1360:1366	arg1	supplement					1368:1377	a gluten-free and lactose-free dietary supplement	1329:1377	a gluten-free and lactose-free dietary supplement that is both nutritive and bioactive, enhancing human health and well-being as well as environmental sustainability	1329:1493	It could generate a gluten-free and lactose-free dietary supplement that is both nutritive and bioactive, enhancing human health and well-being as well as environmental sustainability.					
36374979	8	2	theme	dietary	1360:1366	arg1	nutritive					1392:1400	nutritive	1392:1400	nutritive	1392:1400	It could generate a gluten-free and lactose-free dietary supplement that is both nutritive and bioactive, enhancing human health and well-being as well as environmental sustainability.					
36374979	1	3	theme	Lentinula	258:266	arg1	edodes					268:273	culinary-medicinal mushroom Lentinula edodes	230:273	culinary-medicinal mushroom Lentinula edodes	230:273	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	1	4	theme	dietary	359:365	arg1	fiber					367:371	dietary fiber	359:371	dietary fiber	359:371	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	5	5	theme	protein	1006:1012	arg1	content					1014:1020	high dietary fiber and protein content	983:1020	content	1014:1020	A flour was obtained with high dietary fiber and protein content, and low lipids and carbohydrates content, and low caloric value.					
36374979	4	6	theme	manioc	849:854	arg1	flour					856:860	manioc flour	849:860	manioc flour	849:860	Treatments 4 (with shells, rice bran, and manioc flour) and 6 (with shells, sorghum flour, and manioc flour) presented the highest β-glucans content.					
36374979	2	7	theme	palm	534:537	arg1	shells					539:544	palm shells	534:544	palm shells	534:544	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	1	8	theme	fiber	367:371	arg1	contents					347:354	high contents	342:354	high contents of dietary fiber, protein, and β-glucans	342:395	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	0	9	dep	Products	119:126	arg1	Application					128:138	Application	128:138	Application	128:138	Peach Palm By-Product Bioconversion by Shiitake Culinary-Medicinal Mushroom Lentinula edodes (Agaricomycetes) for Food Products Application in Brazil.					
36374979	5	10	theme	low	1027:1029	arg1	lipids					1031:1036	low lipids	1027:1036	low lipids	1027:1036	A flour was obtained with high dietary fiber and protein content, and low lipids and carbohydrates content, and low caloric value.					
36374979	6	11	theme	curve	1201:1205	arg1	elevation					1175:1183	a lower elevation	1167:1183	a lower elevation of the glycemic curve	1167:1205	The in vivo biological activity demonstrated high protein quality and promoted a lower elevation of the glycemic curve.					
36374979	2	12	theme	mixing	631:636	arg1	plan					638:641	an experimental mixing plan	615:641	an experimental mixing plan	615:641	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	6	13	theme	biological	1100:1109	arg1	activity					1111:1118	The in vivo biological activity	1088:1118	The in vivo biological activity	1088:1118	The in vivo biological activity demonstrated high protein quality and promoted a lower elevation of the glycemic curve.					
36374979	2	14	theme	heart	525:529	arg1	influence					508:516	the influence	504:516	the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour)	504:605	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	1	15	theme	protein	374:380	arg1	contents					347:354	high contents	342:354	high contents of dietary fiber, protein, and β-glucans	342:395	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	4	16	theme	rice	834:837	arg1	bran					839:842	rice bran	834:842	rice bran	834:842	Treatments 4 (with shells, rice bran, and manioc flour) and 6 (with shells, sorghum flour, and manioc flour) presented the highest β-glucans content.					
36374979	2	17	theme	experimental	618:629	arg1	plan					638:641	an experimental mixing plan	615:641	an experimental mixing plan	615:641	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	2	18	theme	β-glucans	402:410	arg1	production					412:421	The β-glucans production	398:421	The β-glucans production by L. edodes mycelium	398:443	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	0	19	theme	Food	114:117	arg1	Products					119:126	Food Products	114:126	Food Products Application in Brazil	114:148	Peach Palm By-Product Bioconversion by Shiitake Culinary-Medicinal Mushroom Lentinula edodes (Agaricomycetes) for Food Products Application in Brazil.					
36374979	3	20	dep	in	778:779	arg1	vivo					781:784	vivo	781:784	vivo	781:784	The cultivation treatment that presented the highest tendency for β-glucans production was analyzed by the centesimal composition and in vivo biological activity.					
36374979	6	21	theme	in	1092:1093	arg1	activity					1111:1118	The in vivo biological activity	1088:1118	The in vivo biological activity	1088:1118	The in vivo biological activity demonstrated high protein quality and promoted a lower elevation of the glycemic curve.					
36374979	7	22	theme	shells	1262:1267	arg1	transformation					1233:1246	the transformation	1229:1246	the transformation of peach palm shells into a food ingredient	1229:1290	Thus, technology for the transformation of peach palm shells into a food ingredient was made feasible.					
36374979	0	23	theme	Palm	6:9	arg1	Bioconversion					22:34	Peach Palm By-Product Bioconversion	0:34	Peach Palm By-Product Bioconversion by Shiitake Culinary-Medicinal Mushroom Lentinula edodes (Agaricomycetes) for Food Products Application in Brazil.	0:149	Peach Palm By-Product Bioconversion by Shiitake Culinary-Medicinal Mushroom Lentinula edodes (Agaricomycetes) for Food Products Application in Brazil.					
36374979	3	24	theme	β-glucans	710:718	arg1	production					720:729	β-glucans production	710:729	β-glucans production	710:729	The cultivation treatment that presented the highest tendency for β-glucans production was analyzed by the centesimal composition and in vivo biological activity.					
36374979	6	25	dep	in	1092:1093	arg1	vivo					1095:1098	vivo	1095:1098	vivo	1095:1098	The in vivo biological activity demonstrated high protein quality and promoted a lower elevation of the glycemic curve.					
36374979	1	26	theme	peach	182:186	arg1	bioconversion					213:225	the peach palm by-product (shells) bioconversion	178:225	the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans	178:395	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	0	27	theme	Peach	0:4	arg1	Bioconversion					22:34	Peach Palm By-Product Bioconversion	0:34	Peach Palm By-Product Bioconversion by Shiitake Culinary-Medicinal Mushroom Lentinula edodes (Agaricomycetes) for Food Products Application in Brazil.	0:149	Peach Palm By-Product Bioconversion by Shiitake Culinary-Medicinal Mushroom Lentinula edodes (Agaricomycetes) for Food Products Application in Brazil.					
36374979	6	28	theme	glycemic	1192:1199	arg1	curve					1201:1205	the glycemic curve	1188:1205	the glycemic curve	1188:1205	The in vivo biological activity demonstrated high protein quality and promoted a lower elevation of the glycemic curve.					
36374979	1	29	contain	containing	331:340	arg1	supplementation					315:329	dietary supplementation	307:329	dietary supplementation containing high contents of dietary fiber, protein, and β-glucans	307:395	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	1	29	contain	containing	331:340	arg2	contents					347:354	high contents	342:354	high contents of dietary fiber, protein, and β-glucans	342:395	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	1	30	theme	palm	188:191	arg1	bioconversion					213:225	the peach palm by-product (shells) bioconversion	178:225	the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans	178:395	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	1	31	theme	food	287:290	arg1	ingredient					292:301	a food ingredient	285:301	a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans	285:395	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	8	32	theme	environmental	1466:1478	arg1	sustainability					1480:1493	environmental sustainability	1466:1493	human health and well-being as well as environmental sustainability	1427:1493	It could generate a gluten-free and lactose-free dietary supplement that is both nutritive and bioactive, enhancing human health and well-being as well as environmental sustainability.					
36374979	1	33	theme	by-product	193:202	arg1	bioconversion					213:225	the peach palm by-product (shells) bioconversion	178:225	the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans	178:395	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	0	34	theme	By-Product	11:20	arg1	Bioconversion					22:34	Peach Palm By-Product Bioconversion	0:34	Peach Palm By-Product Bioconversion by Shiitake Culinary-Medicinal Mushroom Lentinula edodes (Agaricomycetes) for Food Products Application in Brazil.	0:149	Peach Palm By-Product Bioconversion by Shiitake Culinary-Medicinal Mushroom Lentinula edodes (Agaricomycetes) for Food Products Application in Brazil.					
36374979	2	35	theme	supplements	550:560	arg1	influence					508:516	the influence	504:516	the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour)	504:605	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	1	36	theme	β-glucans	387:395	arg1	contents					347:354	high contents	342:354	high contents of dietary fiber, protein, and β-glucans	342:395	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	6	37	theme	high	1133:1136	arg1	quality					1146:1152	high protein quality	1133:1152	high protein quality	1133:1152	The in vivo biological activity demonstrated high protein quality and promoted a lower elevation of the glycemic curve.					
36374979	2	38	theme	L.	426:427	arg1	mycelium					436:443	L. edodes mycelium	426:443	L. edodes mycelium	426:443	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	5	39	theme	low	1069:1071	arg1	value					1081:1085	low caloric value	1069:1085	low caloric value	1069:1085	A flour was obtained with high dietary fiber and protein content, and low lipids and carbohydrates content, and low caloric value.					
36374979	5	40	dep	lipids	1031:1036	arg1	content					1056:1062	content	1056:1062	content	1056:1062	A flour was obtained with high dietary fiber and protein content, and low lipids and carbohydrates content, and low caloric value.					
36374979	6	41	theme	lower	1169:1173	arg1	elevation					1175:1183	a lower elevation	1167:1183	a lower elevation of the glycemic curve	1167:1205	The in vivo biological activity demonstrated high protein quality and promoted a lower elevation of the glycemic curve.					
36374979	7	42	theme	peach	1251:1255	arg1	shells					1262:1267	peach palm shells	1251:1267	peach palm shells	1251:1267	Thus, technology for the transformation of peach palm shells into a food ingredient was made feasible.					
36374979	1	43	theme	shells	205:210	arg1	bioconversion					213:225	the peach palm by-product (shells) bioconversion	178:225	the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans	178:395	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	4	44	theme	β-glucans	938:946	arg1	content					948:954	the highest β-glucans content	926:954	the highest β-glucans content	926:954	Treatments 4 (with shells, rice bran, and manioc flour) and 6 (with shells, sorghum flour, and manioc flour) presented the highest β-glucans content.					
36374979	1	45	theme	dietary	307:313	arg1	supplementation					315:329	dietary supplementation	307:329	dietary supplementation containing high contents of dietary fiber, protein, and β-glucans	307:395	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	2	46	theme	shells	539:544	arg1	influence					508:516	the influence	504:516	the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour)	504:605	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	7	47	theme	palm	1257:1260	arg1	shells					1262:1267	peach palm shells	1251:1267	peach palm shells	1251:1267	Thus, technology for the transformation of peach palm shells into a food ingredient was made feasible.					
36374979	3	48	theme	biological	786:795	arg1	activity					797:804	in vivo biological activity	778:804	in vivo biological activity	778:804	The cultivation treatment that presented the highest tendency for β-glucans production was analyzed by the centesimal composition and in vivo biological activity.					
36374979	2	49	dep	supplements	550:560	arg1	supplements					550:560	supplements	550:560	supplements (rice bran, manioc flour, and sorghum flour)	550:605	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	2	49	dep	supplements	550:560	arg1	bran					568:571	rice bran	563:571	rice bran	563:571	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	2	49	dep	supplements	550:560	arg1	flour					581:585	manioc flour	574:585	manioc flour	574:585	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	2	49	dep	supplements	550:560	arg1	flour					600:604	sorghum flour	592:604	sorghum flour	592:604	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	2	50	theme	solid-state	469:479	arg1	fermentation					481:492	a solid-state fermentation	467:492	a solid-state fermentation	467:492	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	3	51	theme	in	778:779	arg1	activity					797:804	in vivo biological activity	778:804	in vivo biological activity	778:804	The cultivation treatment that presented the highest tendency for β-glucans production was analyzed by the centesimal composition and in vivo biological activity.					
36374979	2	52	theme	manioc	574:579	arg1	supplements					550:560	supplements	550:560	supplements (rice bran, manioc flour, and sorghum flour)	550:605	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	2	52	theme	manioc	574:579	arg1	flour					581:585	manioc flour	574:585	manioc flour	574:585	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	3	53	theme	centesimal	751:760	arg1	composition					762:772	the centesimal composition	747:772	the centesimal composition	747:772	The cultivation treatment that presented the highest tendency for β-glucans production was analyzed by the centesimal composition and in vivo biological activity.					
36374979	4	54	theme	sorghum	883:889	arg1	flour					891:895	sorghum flour	883:895	sorghum flour	883:895	Treatments 4 (with shells, rice bran, and manioc flour) and 6 (with shells, sorghum flour, and manioc flour) presented the highest β-glucans content.					
36374979	8	55	theme	human	1427:1431	arg1	health					1433:1438	human health	1427:1438	human health	1427:1438	It could generate a gluten-free and lactose-free dietary supplement that is both nutritive and bioactive, enhancing human health and well-being as well as environmental sustainability.					
36374979	5	56	theme	high	983:986	arg1	fiber					996:1000	high dietary fiber and protein content	983:1020	fiber	996:1000	A flour was obtained with high dietary fiber and protein content, and low lipids and carbohydrates content, and low caloric value.					
36374979	8	57	theme	lactose-free	1347:1358	arg1	supplement					1368:1377	a gluten-free and lactose-free dietary supplement	1329:1377	a gluten-free and lactose-free dietary supplement that is both nutritive and bioactive, enhancing human health and well-being as well as environmental sustainability	1329:1493	It could generate a gluten-free and lactose-free dietary supplement that is both nutritive and bioactive, enhancing human health and well-being as well as environmental sustainability.					
36374979	8	57	theme	lactose-free	1347:1358	arg1	nutritive					1392:1400	nutritive	1392:1400	nutritive	1392:1400	It could generate a gluten-free and lactose-free dietary supplement that is both nutritive and bioactive, enhancing human health and well-being as well as environmental sustainability.					
36374979	5	58	theme	caloric	1073:1079	arg1	value					1081:1085	low caloric value	1069:1085	low caloric value	1069:1085	A flour was obtained with high dietary fiber and protein content, and low lipids and carbohydrates content, and low caloric value.					
36374979	1	59	theme	high	342:345	arg1	contents					347:354	high contents	342:354	high contents of dietary fiber, protein, and β-glucans	342:395	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	5	60	theme	dietary	988:994	arg1	fiber					996:1000	high dietary fiber and protein content	983:1020	fiber	996:1000	A flour was obtained with high dietary fiber and protein content, and low lipids and carbohydrates content, and low caloric value.					
36374979	7	61	theme	food	1276:1279	arg1	ingredient					1281:1290	a food ingredient	1274:1290	a food ingredient	1274:1290	Thus, technology for the transformation of peach palm shells into a food ingredient was made feasible.					
36374979	2	62	theme	rice	563:566	arg1	supplements					550:560	supplements	550:560	supplements (rice bran, manioc flour, and sorghum flour)	550:605	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	2	62	theme	rice	563:566	arg1	bran					568:571	rice bran	563:571	rice bran	563:571	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	6	63	theme	protein	1138:1144	arg1	quality					1146:1152	high protein quality	1133:1152	high protein quality	1133:1152	The in vivo biological activity demonstrated high protein quality and promoted a lower elevation of the glycemic curve.					
36374979	8	64	theme	gluten-free	1331:1341	arg1	supplement					1368:1377	a gluten-free and lactose-free dietary supplement	1329:1377	a gluten-free and lactose-free dietary supplement that is both nutritive and bioactive, enhancing human health and well-being as well as environmental sustainability	1329:1493	It could generate a gluten-free and lactose-free dietary supplement that is both nutritive and bioactive, enhancing human health and well-being as well as environmental sustainability.					
36374979	8	64	theme	gluten-free	1331:1341	arg1	nutritive					1392:1400	nutritive	1392:1400	nutritive	1392:1400	It could generate a gluten-free and lactose-free dietary supplement that is both nutritive and bioactive, enhancing human health and well-being as well as environmental sustainability.					
36374979	3	65	theme	cultivation	648:658	arg1	treatment					660:668	The cultivation treatment	644:668	The cultivation treatment that presented the highest tendency for β-glucans production	644:729	The cultivation treatment that presented the highest tendency for β-glucans production was analyzed by the centesimal composition and in vivo biological activity.					
36374979	4	66	dep	Treatments	807:816	arg1	6					867:867	6	867:867	6	867:867	Treatments 4 (with shells, rice bran, and manioc flour) and 6 (with shells, sorghum flour, and manioc flour) presented the highest β-glucans content.					
36374979	4	66	dep	Treatments	807:816	arg1	4					818:818	4	818:818	4	818:818	Treatments 4 (with shells, rice bran, and manioc flour) and 6 (with shells, sorghum flour, and manioc flour) presented the highest β-glucans content.					
36374979	2	67	theme	edodes	429:434	arg1	mycelium					436:443	L. edodes mycelium	426:443	L. edodes mycelium	426:443	The β-glucans production by L. edodes mycelium was optimized through a solid-state fermentation, checking the influence of the heart of palm shells and supplements (rice bran, manioc flour, and sorghum flour) through an experimental mixing plan.					
36374979	1	68	theme	culinary-medicinal	230:247	arg1	edodes					268:273	culinary-medicinal mushroom Lentinula edodes	230:273	culinary-medicinal mushroom Lentinula edodes	230:273	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
36374979	3	69	theme	highest	689:695	arg1	tendency					697:704	the highest tendency	685:704	the highest tendency for β-glucans production	685:729	The cultivation treatment that presented the highest tendency for β-glucans production was analyzed by the centesimal composition and in vivo biological activity.					
36374979	0	70	from	Application	128:138	arg1	Brazil					143:148	Brazil	143:148	Brazil	143:148	Peach Palm By-Product Bioconversion by Shiitake Culinary-Medicinal Mushroom Lentinula edodes (Agaricomycetes) for Food Products Application in Brazil.					
36374979	4	71	theme	manioc	902:907	arg1	flour					909:913	manioc flour	902:913	manioc flour	902:913	Treatments 4 (with shells, rice bran, and manioc flour) and 6 (with shells, sorghum flour, and manioc flour) presented the highest β-glucans content.					
36374979	1	72	theme	mushroom	249:256	arg1	edodes					268:273	culinary-medicinal mushroom Lentinula edodes	230:273	culinary-medicinal mushroom Lentinula edodes	230:273	This study aims to analyze the peach palm by-product (shells) bioconversion by culinary-medicinal mushroom Lentinula edodes to obtain a food ingredient for dietary supplementation containing high contents of dietary fiber, protein, and β-glucans.					
35253986	7	0	theme	bacterial	987:995	arg1	suspension					997:1006	the bacterial suspension	983:1006	the bacterial suspension	983:1006	The bacterial environment model was established by injection of the bacterial suspension.					
35253986	8	1	from	morphology	1013:1022	arg1	surface					1053:1059	the surface	1049:1059	the surface of catheters and the surrounding tissues	1049:1100	The morphology and bacterial adhered on the surface of catheters and the surrounding tissues were analyzed after 5 days of implantation.					
35253986	9	2	theme	in	1149:1150	arg1	results					1158:1164	in vitro results	1149:1164	in vitro results	1149:1164	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	9	2	theme	in	1149:1150	arg1	number					1171:1176	the number	1167:1176	the number of adhered bacterial on the surface of the silicon rubber surface	1167:1242	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	1	3	theme	rubber	233:238	arg1	surface					240:246	the silicone rubber surface	220:246	the silicone rubber surface	220:246	Copper ions (Cu) grafted chitosan coating was prepared using the pneumatic spraying method on the silicone rubber surface.					
35253986	8	4	theme	surrounding	1082:1092	arg1	tissues					1094:1100	the surrounding tissues	1078:1100	the surrounding tissues	1078:1100	The morphology and bacterial adhered on the surface of catheters and the surrounding tissues were analyzed after 5 days of implantation.					
35253986	12	5	theme	antibacterial	1800:1812	arg1	activity					1836:1843	excellent antibacterial and anti-inflammatory activity	1790:1843	excellent antibacterial and anti-inflammatory activity	1790:1843	Results indicate that the Cu-modified chitosan coating can confer excellent antibacterial and anti-inflammatory activity as applied on medical catheters.					
35253986	11	6	theme	injected	1662:1669	arg1	samples					1671:1677	other injected samples	1656:1677	other injected samples	1656:1677	In the injected chitosan/Cu coated group; the inflammation, the number of the adhered bacteria were observed less than other injected samples without Cu; no inflammation were noticeable.					
35253986	1	7	theme	grafted	143:149	arg1	coating					160:166	Copper ions (Cu) grafted chitosan coating	126:166	Copper ions (Cu) grafted chitosan coating	126:166	Copper ions (Cu) grafted chitosan coating was prepared using the pneumatic spraying method on the silicone rubber surface.					
35253986	10	8	theme	surrounding	1455:1465	arg1	tissues					1467:1473	the surrounding tissues	1451:1473	the surrounding tissues of all catheters in all without the S. aureus injected group	1451:1534	As for in vivo results, after 5 days of implantation, there was no evident inflammation in the surrounding tissues of all catheters in all without the S. aureus injected group.					
35253986	9	9	theme	silicon	1221:1227	arg1	rubber					1229:1234	silicon rubber	1221:1234	the silicon rubber surface	1217:1242	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	10	10	theme	in	1367:1368	arg1	results					1375:1381	in vivo results	1367:1381	in vivo results	1367:1381	As for in vivo results, after 5 days of implantation, there was no evident inflammation in the surrounding tissues of all catheters in all without the S. aureus injected group.					
35253986	6	11	dep	activity	770:777	arg1	the					748:750	the	748:750	the	748:750	To evaluate the anti-inflammatory activity and biosafety of the coating in vivo, the optimized coating samples and control groups were implanted subcutaneously into the back of mice.					
35253986	10	12	from	all	1495:1497	arg1	tissues					1467:1473	the surrounding tissues	1451:1473	the surrounding tissues of all catheters in all without the S. aureus injected group	1451:1534	As for in vivo results, after 5 days of implantation, there was no evident inflammation in the surrounding tissues of all catheters in all without the S. aureus injected group.					
35253986	11	13	theme	adhered	1615:1621	arg1	bacteria					1623:1630	the adhered bacteria	1611:1630	the adhered bacteria	1611:1630	In the injected chitosan/Cu coated group; the inflammation, the number of the adhered bacteria were observed less than other injected samples without Cu; no inflammation were noticeable.					
35253986	11	14	theme	bacteria	1623:1630	arg1	number					1601:1606	the number	1597:1606	the number of the adhered bacteria	1597:1630	In the injected chitosan/Cu coated group; the inflammation, the number of the adhered bacteria were observed less than other injected samples without Cu; no inflammation were noticeable.					
35253986	9	15	from	coating	1351:1357	arg1	intensify					1311:1319	the intensify	1307:1319	the intensify of the Cu content in chitosan coating	1307:1357	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	9	16	theme	bacterial	1189:1197	arg1	results					1158:1164	in vitro results	1149:1164	in vitro results	1149:1164	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	9	16	theme	bacterial	1189:1197	arg1	number					1171:1176	the number	1167:1176	the number of adhered bacterial on the surface of the silicon rubber surface	1167:1242	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	9	17	dep	in	1149:1150	arg1	vitro					1152:1156	vitro	1152:1156	vitro	1152:1156	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	3	18	theme	electron	485:492	arg1	microscopy					494:503	scanning electron microscopy	476:503	scanning electron microscopy (SEM)	476:509	Surface properties, composition, and morphology were investigated by scanning electron microscopy (SEM) and contact angle measurements.					
35253986	3	18	theme	electron	485:492	arg1	SEM					506:508	SEM	506:508	SEM	506:508	Surface properties, composition, and morphology were investigated by scanning electron microscopy (SEM) and contact angle measurements.					
35253986	12	19	theme	Cu-modified	1750:1760	arg1	coating					1771:1777	the Cu-modified chitosan coating	1746:1777	the Cu-modified chitosan coating	1746:1777	Results indicate that the Cu-modified chitosan coating can confer excellent antibacterial and anti-inflammatory activity as applied on medical catheters.					
35253986	8	20	theme	catheters	1064:1072	arg1	surface					1053:1059	the surface	1049:1059	the surface of catheters and the surrounding tissues	1049:1100	The morphology and bacterial adhered on the surface of catheters and the surrounding tissues were analyzed after 5 days of implantation.					
35253986	9	21	from	content	1331:1337	arg1	coating					1351:1357	chitosan coating	1342:1357	chitosan coating	1342:1357	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	2	22	theme	surface	259:265	arg1	composition					291:301	composition	291:301	composition	291:301	Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed.					
35253986	2	22	theme	surface	259:265	arg1	antibacterial					327:339	antibacterial	327:339	antibacterial	327:339	Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed.					
35253986	2	22	theme	surface	259:265	arg1	properties					267:276	Coating's surface properties	249:276	Coating's surface properties	249:276	Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed.					
35253986	2	22	theme	surface	259:265	arg1	behavior					317:324	Cu releasing behavior	304:324	Cu releasing behavior	304:324	Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed.					
35253986	2	22	theme	surface	259:265	arg1	activities					364:373	anti-inflammatory activities	346:373	anti-inflammatory activities	346:373	Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed.					
35253986	2	22	theme	surface	259:265	arg1	morphology					279:288	morphology	279:288	morphology	279:288	Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed.					
35253986	11	23	theme	coated	1565:1570	arg1	group					1572:1576	the injected chitosan/Cu coated group	1540:1576	the injected chitosan/Cu coated group; the inflammation	1540:1594	In the injected chitosan/Cu coated group; the inflammation, the number of the adhered bacteria were observed less than other injected samples without Cu; no inflammation were noticeable.					
35253986	1	24	theme	chitosan	151:158	arg1	coating					160:166	Copper ions (Cu) grafted chitosan coating	126:166	Copper ions (Cu) grafted chitosan coating	126:166	Copper ions (Cu) grafted chitosan coating was prepared using the pneumatic spraying method on the silicone rubber surface.					
35253986	10	25	theme	injected	1521:1528	arg1	group					1530:1534	the S. aureus injected group	1507:1534	the S. aureus injected group	1507:1534	As for in vivo results, after 5 days of implantation, there was no evident inflammation in the surrounding tissues of all catheters in all without the S. aureus injected group.					
35253986	12	26	theme	medical	1859:1865	arg1	catheters					1867:1875	medical catheters	1859:1875	medical catheters	1859:1875	Results indicate that the Cu-modified chitosan coating can confer excellent antibacterial and anti-inflammatory activity as applied on medical catheters.					
35253986	3	27	theme	Surface	407:413	arg1	properties					415:424	Surface properties	407:424	Surface properties	407:424	Surface properties, composition, and morphology were investigated by scanning electron microscopy (SEM) and contact angle measurements.					
35253986	6	28	theme	coating	800:806	arg1	biosafety					783:791	biosafety	783:791	biosafety	783:791	To evaluate the anti-inflammatory activity and biosafety of the coating in vivo, the optimized coating samples and control groups were implanted subcutaneously into the back of mice.					
35253986	6	28	theme	coating	800:806	arg1	activity					770:777	anti-inflammatory activity	752:777	anti-inflammatory activity	752:777	To evaluate the anti-inflammatory activity and biosafety of the coating in vivo, the optimized coating samples and control groups were implanted subcutaneously into the back of mice.					
35253986	8	29	dep	morphology	1013:1022	arg1	The					1009:1011	The	1009:1011	The	1009:1011	The morphology and bacterial adhered on the surface of catheters and the surrounding tissues were analyzed after 5 days of implantation.					
35253986	9	30	from	number	1171:1176	arg1	surface					1206:1212	the surface	1202:1212	the surface of the silicon rubber surface	1202:1242	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	9	31	from	intensify	1311:1319	arg1	coating					1351:1357	chitosan coating	1342:1357	chitosan coating	1342:1357	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	10	32	from	catheters	1482:1490	arg1	all					1495:1497	all	1495:1497	all	1495:1497	As for in vivo results, after 5 days of implantation, there was no evident inflammation in the surrounding tissues of all catheters in all without the S. aureus injected group.					
35253986	10	33	theme	evident	1427:1433	arg1	inflammation					1435:1446	no evident inflammation	1424:1446	no evident inflammation	1424:1446	As for in vivo results, after 5 days of implantation, there was no evident inflammation in the surrounding tissues of all catheters in all without the S. aureus injected group.					
35253986	5	34	theme	biofilm	687:693	arg1	morphology					669:678	the morphology	665:678	the morphology of the biofilm	665:693	Besides, the morphology of the biofilm was inspected with a field emission SEM.					
35253986	0	35	theme	Antibacterial	0:12	arg1	activities					36:45	Antibacterial and anti-inflammatory activities	0:45	Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo.	0:124	Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo.					
35253986	7	36	theme	bacterial	923:931	arg1	model					945:949	The bacterial environment model	919:949	The bacterial environment model	919:949	The bacterial environment model was established by injection of the bacterial suspension.					
35253986	1	37	dep	grafted	143:149	arg1	ions					133:136	Copper ions	126:136	Copper ions	126:136	Copper ions (Cu) grafted chitosan coating was prepared using the pneumatic spraying method on the silicone rubber surface.					
35253986	11	38	dep	group	1572:1576	arg1	inflammation					1583:1594	the inflammation	1579:1594	the injected chitosan/Cu coated group; the inflammation	1540:1594	In the injected chitosan/Cu coated group; the inflammation, the number of the adhered bacteria were observed less than other injected samples without Cu; no inflammation were noticeable.					
35253986	11	39	theme	other	1656:1660	arg1	samples					1671:1677	other injected samples	1656:1677	other injected samples	1656:1677	In the injected chitosan/Cu coated group; the inflammation, the number of the adhered bacteria were observed less than other injected samples without Cu; no inflammation were noticeable.					
35253986	4	40	theme	antibacterial	547:559	arg1	activity					561:568	The antibacterial activity	543:568	The antibacterial activity	543:568	The antibacterial activity has been tested with Escherichia coli and Staphylococcus aureus suspensions in vitro.					
35253986	8	41	theme	tissues	1094:1100	arg1	surface					1053:1059	the surface	1049:1059	the surface of catheters and the surrounding tissues	1049:1100	The morphology and bacterial adhered on the surface of catheters and the surrounding tissues were analyzed after 5 days of implantation.					
35253986	7	42	theme	environment	933:943	arg1	model					945:949	The bacterial environment model	919:949	The bacterial environment model	919:949	The bacterial environment model was established by injection of the bacterial suspension.					
35253986	12	43	theme	excellent	1790:1798	arg1	activity					1836:1843	excellent antibacterial and anti-inflammatory activity	1790:1843	excellent antibacterial and anti-inflammatory activity	1790:1843	Results indicate that the Cu-modified chitosan coating can confer excellent antibacterial and anti-inflammatory activity as applied on medical catheters.					
35253986	0	44	theme	anti-inflammatory	18:34	arg1	activities					36:45	Antibacterial and anti-inflammatory activities	0:45	Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo.	0:124	Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo.					
35253986	9	45	theme	content	1331:1337	arg1	intensify					1311:1319	the intensify	1307:1319	the intensify of the Cu content in chitosan coating	1307:1357	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	3	46	theme	angle	523:527	arg1	measurements					529:540	contact angle measurements	515:540	contact angle measurements	515:540	Surface properties, composition, and morphology were investigated by scanning electron microscopy (SEM) and contact angle measurements.					
35253986	4	47	dep	coli	603:606	arg1	suspensions					634:644	suspensions	634:644	suspensions	634:644	The antibacterial activity has been tested with Escherichia coli and Staphylococcus aureus suspensions in vitro.					
35253986	0	48	theme	chitosan/copper	50:64	arg1	complex					66:72	chitosan/copper complex	50:72	chitosan/copper complex coating on medical catheters	50:101	Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo.					
35253986	5	49	theme	field	716:720	arg1	SEM					731:733	a field emission SEM	714:733	a field emission SEM	714:733	Besides, the morphology of the biofilm was inspected with a field emission SEM.					
35253986	9	50	theme	rubber	1229:1234	arg1	surface					1236:1242	the silicon rubber surface	1217:1242	the silicon rubber surface	1217:1242	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	11	51	located	observed	1637:1644	arg1	group					1572:1576	the injected chitosan/Cu coated group	1540:1576	the injected chitosan/Cu coated group; the inflammation	1540:1594	In the injected chitosan/Cu coated group; the inflammation, the number of the adhered bacteria were observed less than other injected samples without Cu; no inflammation were noticeable.					
35253986	11	51	located	observed	1637:1644	arg2	number					1601:1606	the number	1597:1606	the number of the adhered bacteria	1597:1630	In the injected chitosan/Cu coated group; the inflammation, the number of the adhered bacteria were observed less than other injected samples without Cu; no inflammation were noticeable.					
35253986	1	52	theme	pneumatic	191:199	arg1	method					210:215	the pneumatic spraying method	187:215	the pneumatic spraying method on the silicone rubber surface	187:246	Copper ions (Cu) grafted chitosan coating was prepared using the pneumatic spraying method on the silicone rubber surface.					
35253986	9	53	theme	chitosan	1342:1349	arg1	coating					1351:1357	chitosan coating	1342:1357	chitosan coating	1342:1357	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	5	54	theme	emission	722:729	arg1	SEM					731:733	a field emission SEM	714:733	a field emission SEM	714:733	Besides, the morphology of the biofilm was inspected with a field emission SEM.					
35253986	9	55	theme	surface	1236:1242	arg1	surface					1206:1212	the surface	1202:1212	the surface of the silicon rubber surface	1202:1242	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	9	56	theme	Cu	1328:1329	arg1	content					1331:1337	the Cu content	1324:1337	the Cu content in chitosan coating	1324:1357	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	11	57	theme	chitosan/Cu	1553:1563	arg1	group					1572:1576	the injected chitosan/Cu coated group	1540:1576	the injected chitosan/Cu coated group; the inflammation	1540:1594	In the injected chitosan/Cu coated group; the inflammation, the number of the adhered bacteria were observed less than other injected samples without Cu; no inflammation were noticeable.					
35253986	1	58	theme	spraying	201:208	arg1	method					210:215	the pneumatic spraying method	187:215	the pneumatic spraying method on the silicone rubber surface	187:246	Copper ions (Cu) grafted chitosan coating was prepared using the pneumatic spraying method on the silicone rubber surface.					
35253986	10	59	theme	implantation	1400:1411	arg1	5 days					1390:1395	5 days	1390:1395	5 days of implantation	1390:1411	As for in vivo results, after 5 days of implantation, there was no evident inflammation in the surrounding tissues of all catheters in all without the S. aureus injected group.					
35253986	6	60	theme	control	851:857	arg1	groups					859:864	control groups	851:864	control groups	851:864	To evaluate the anti-inflammatory activity and biosafety of the coating in vivo, the optimized coating samples and control groups were implanted subcutaneously into the back of mice.					
35253986	8	61	theme	implantation	1132:1143	arg1	5 days					1122:1127	5 days	1122:1127	5 days of implantation	1122:1143	The morphology and bacterial adhered on the surface of catheters and the surrounding tissues were analyzed after 5 days of implantation.					
35253986	0	62	theme	complex	66:72	arg1	activities					36:45	Antibacterial and anti-inflammatory activities	0:45	Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo.	0:124	Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo.					
35253986	3	63	theme	scanning	476:483	arg1	microscopy					494:503	scanning electron microscopy	476:503	scanning electron microscopy (SEM)	476:509	Surface properties, composition, and morphology were investigated by scanning electron microscopy (SEM) and contact angle measurements.					
35253986	3	63	theme	scanning	476:483	arg1	SEM					506:508	SEM	506:508	SEM	506:508	Surface properties, composition, and morphology were investigated by scanning electron microscopy (SEM) and contact angle measurements.					
35253986	6	64	theme	anti-inflammatory	752:768	arg1	activity					770:777	anti-inflammatory activity	752:777	anti-inflammatory activity	752:777	To evaluate the anti-inflammatory activity and biosafety of the coating in vivo, the optimized coating samples and control groups were implanted subcutaneously into the back of mice.					
35253986	1	65	from	method	210:215	arg1	surface					240:246	the silicone rubber surface	220:246	the silicone rubber surface	220:246	Copper ions (Cu) grafted chitosan coating was prepared using the pneumatic spraying method on the silicone rubber surface.					
35253986	0	66	dep	activities	36:45	arg1	vitro					107:111	In vitro	104:111	In vitro	104:111	Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo.					
35253986	0	66	dep	activities	36:45	arg1	vivo					120:123	in vivo	117:123	in vivo	117:123	Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo.					
35253986	11	67	theme	injected	1544:1551	arg1	group					1572:1576	the injected chitosan/Cu coated group	1540:1576	the injected chitosan/Cu coated group; the inflammation	1540:1594	In the injected chitosan/Cu coated group; the inflammation, the number of the adhered bacteria were observed less than other injected samples without Cu; no inflammation were noticeable.					
35253986	1	68	theme	Copper	126:131	arg1	ions					133:136	Copper ions	126:136	Copper ions	126:136	Copper ions (Cu) grafted chitosan coating was prepared using the pneumatic spraying method on the silicone rubber surface.					
35253986	12	69	theme	anti-inflammatory	1818:1834	arg1	activity					1836:1843	excellent antibacterial and anti-inflammatory activity	1790:1843	excellent antibacterial and anti-inflammatory activity	1790:1843	Results indicate that the Cu-modified chitosan coating can confer excellent antibacterial and anti-inflammatory activity as applied on medical catheters.					
35253986	6	70	theme	coating	831:837	arg1	samples					839:845	the optimized coating samples	817:845	the optimized coating samples	817:845	To evaluate the anti-inflammatory activity and biosafety of the coating in vivo, the optimized coating samples and control groups were implanted subcutaneously into the back of mice.					
35253986	7	71	theme	suspension	997:1006	arg1	injection					970:978	injection	970:978	injection of the bacterial suspension	970:1006	The bacterial environment model was established by injection of the bacterial suspension.					
35253986	2	72	theme	Cu	304:305	arg1	behavior					317:324	Cu releasing behavior	304:324	Cu releasing behavior	304:324	Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed.					
35253986	2	72	theme	Cu	304:305	arg1	properties					267:276	Coating's surface properties	249:276	Coating's surface properties	249:276	Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed.					
35253986	2	73	theme	releasing	307:315	arg1	behavior					317:324	Cu releasing behavior	304:324	Cu releasing behavior	304:324	Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed.					
35253986	2	73	theme	releasing	307:315	arg1	properties					267:276	Coating's surface properties	249:276	Coating's surface properties	249:276	Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed.					
35253986	0	74	theme	medical	85:91	arg1	catheters					93:101	medical catheters	85:101	medical catheters	85:101	Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo.					
35253986	6	75	theme	optimized	821:829	arg1	samples					839:845	the optimized coating samples	817:845	the optimized coating samples	817:845	To evaluate the anti-inflammatory activity and biosafety of the coating in vivo, the optimized coating samples and control groups were implanted subcutaneously into the back of mice.					
35253986	10	76	theme	catheters	1482:1490	arg1	tissues					1467:1473	the surrounding tissues	1451:1473	the surrounding tissues of all catheters in all without the S. aureus injected group	1451:1534	As for in vivo results, after 5 days of implantation, there was no evident inflammation in the surrounding tissues of all catheters in all without the S. aureus injected group.					
35253986	2	77	theme	anti-inflammatory	346:362	arg1	properties					267:276	Coating's surface properties	249:276	Coating's surface properties	249:276	Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed.					
35253986	2	77	theme	anti-inflammatory	346:362	arg1	activities					364:373	anti-inflammatory activities	346:373	anti-inflammatory activities	346:373	Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed.					
35253986	9	78	theme	anti-inflammatory	1267:1283	arg1	rate					1285:1288	the anti-inflammatory rate	1263:1288	the anti-inflammatory rate	1263:1288	As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating.					
35253986	10	79	from	tissues	1467:1473	arg1	all					1495:1497	all	1495:1497	all	1495:1497	As for in vivo results, after 5 days of implantation, there was no evident inflammation in the surrounding tissues of all catheters in all without the S. aureus injected group.					
35253986	12	80	theme	chitosan	1762:1769	arg1	coating					1771:1777	the Cu-modified chitosan coating	1746:1777	the Cu-modified chitosan coating	1746:1777	Results indicate that the Cu-modified chitosan coating can confer excellent antibacterial and anti-inflammatory activity as applied on medical catheters.					
35253986	3	81	theme	contact	515:521	arg1	measurements					529:540	contact angle measurements	515:540	contact angle measurements	515:540	Surface properties, composition, and morphology were investigated by scanning electron microscopy (SEM) and contact angle measurements.					
35253986	10	82	dep	in	1367:1368	arg1	vivo					1370:1373	vivo	1370:1373	vivo	1370:1373	As for in vivo results, after 5 days of implantation, there was no evident inflammation in the surrounding tissues of all catheters in all without the S. aureus injected group.					
35253986	1	83	theme	silicone	224:231	arg1	surface					240:246	the silicone rubber surface	220:246	the silicone rubber surface	220:246	Copper ions (Cu) grafted chitosan coating was prepared using the pneumatic spraying method on the silicone rubber surface.					
37108723	7	0	theme	mechanical	1123:1132	arg1	properties					1134:1143	good mechanical properties	1118:1143	good mechanical properties according to rheological tests	1118:1174	They show good swelling and have good mechanical properties according to rheological tests.					
37108723	0	1	dep	Cleanup	120:126	arg1	Safeguarding					144:155	Safeguarding	144:155	Safeguarding	144:155	Composite Hydrogels Based on Poly(Ethylene Glycol) and Cellulose Macromonomers as Fortified Materials for Environmental Cleanup and Clean Water Safeguarding.					
37108723	5	2	attach	derived	933:939	arg2	products					924:931	products	924:931	products derived from natural renewable resources	924:972	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	5	2	attach	derived	933:939	arg1	resources					964:972	natural renewable resources	946:972	natural renewable resources	946:972	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	0	3	theme	Cellulose	55:63	arg1	Macromonomers					65:77	Poly(Ethylene Glycol) and Cellulose Macromonomers	29:77	Poly(Ethylene Glycol) and Cellulose Macromonomers as Fortified Materials for Environmental Cleanup and Clean Water Safeguarding	29:155	Composite Hydrogels Based on Poly(Ethylene Glycol) and Cellulose Macromonomers as Fortified Materials for Environmental Cleanup and Clean Water Safeguarding.					
37108723	1	4	with	Pollution	158:166	arg1	dyes					181:184	organic dyes	173:184	organic dyes	173:184	Pollution with organic dyes is one of the most typical environmental problems related to industrial wastewater.					
37108723	3	5	theme	hydrogels	507:515	arg1	synthesis					478:486	the synthesis	474:486	the synthesis of novel fortified hydrogels that can bind and remove organic dyes from aqueous solutions	474:576	This paper reports the synthesis of novel fortified hydrogels that can bind and remove organic dyes from aqueous solutions.					
37108723	6	6	dep	good	1047:1050	arg1	to					1058:1059	to	1058:1059	to	1058:1059	The networks are formed with good (75%) to excellent (96%) yields.					
37108723	6	6	dep	good	1047:1050	arg1	%					1055:1055	75%	1053:1055	75%	1053:1055	The networks are formed with good (75%) to excellent (96%) yields.					
37108723	6	6	dep	good	1047:1050	arg1	excellent					1061:1069	excellent	1061:1069	excellent	1061:1069	The networks are formed with good (75%) to excellent (96%) yields.					
37108723	6	6	dep	good	1047:1050	arg1	%					1074:1074	96%	1072:1074	96%	1072:1074	The networks are formed with good (75%) to excellent (96%) yields.					
37108723	1	7	theme	industrial	247:256	arg1	wastewater					258:267	industrial wastewater	247:267	industrial wastewater	247:267	Pollution with organic dyes is one of the most typical environmental problems related to industrial wastewater.					
37108723	0	8	theme	Fortified	82:90	arg1	Materials					92:100	Fortified Materials	82:100	Fortified Materials for Environmental Cleanup and Clean Water Safeguarding	82:155	Composite Hydrogels Based on Poly(Ethylene Glycol) and Cellulose Macromonomers as Fortified Materials for Environmental Cleanup and Clean Water Safeguarding.					
37108723	5	9	theme	Williamson	729:738	arg1	etherification					740:753	Williamson etherification	729:753	Williamson etherification with 4-vinylbenzyl chloride (4-VBC)	729:789	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	5	10	theme	Technocell™	896:906	arg1	cellulose					913:921	Technocell™ T-90 cellulose	896:921	Technocell™ T-90 cellulose (products derived from natural renewable resources)	896:973	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	3	11	theme	aqueous	560:566	arg1	solutions					568:576	aqueous solutions	560:576	aqueous solutions	560:576	This paper reports the synthesis of novel fortified hydrogels that can bind and remove organic dyes from aqueous solutions.					
37108723	3	12	theme	organic	542:548	arg1	dyes					550:553	organic dyes	542:553	organic dyes from aqueous solutions	542:576	This paper reports the synthesis of novel fortified hydrogels that can bind and remove organic dyes from aqueous solutions.					
37108723	3	13	theme	fortified	497:505	arg1	hydrogels					507:515	novel fortified hydrogels	491:515	novel fortified hydrogels that can bind and remove organic dyes from aqueous solutions	491:576	This paper reports the synthesis of novel fortified hydrogels that can bind and remove organic dyes from aqueous solutions.					
37108723	4	14	theme	hydrophilic	585:595	arg1	conetworks					597:606	These hydrophilic conetworks	579:606	These hydrophilic conetworks	579:606	These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers ("cellu-mers").					
37108723	4	15	theme	cellulose	689:697	arg1	macromonomers					699:711	multifunctional cellulose macromonomers	673:711	multifunctional cellulose macromonomers ("cellu-mers")	673:726	These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers ("cellu-mers").					
37108723	4	15	theme	cellulose	689:697	arg1	"					725:725	"cellu-mers"	714:725	"cellu-mers"	714:725	These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers ("cellu-mers").					
37108723	9	16	theme	blue	1385:1388	arg1	MB					1391:1392	MB	1391:1392	MB	1391:1392	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	9	16	theme	blue	1385:1388	arg1	methylene					1375:1383	methylene blue	1375:1388	methylene blue (MB)	1375:1393	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	9	17	theme	clean	1529:1533	arg1	water					1535:1539	clean water safeguarding	1529:1552	clean water safeguarding	1529:1552	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	5	18	theme	molecular	827:835	arg1	masses					837:842	different molecular masses	817:842	different molecular masses (1, 5, 6, and 10 kDa)	817:864	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	5	18	theme	molecular	827:835	arg1	kDa					861:863	1, 5, 6, and 10 kDa	845:863	1, 5, 6, and 10 kDa	845:863	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	8	19	theme	hydrogel	1278:1285	arg1	structure					1287:1295	the inner hydrogel structure	1268:1295	the inner hydrogel structure	1268:1295	Scanning electron microscopy (SEM) reveals that cellulose fibers are visibly embedded into the inner hydrogel structure.					
37108723	8	20	theme	Scanning	1177:1184	arg1	microscopy					1195:1204	Scanning electron microscopy	1177:1204	Scanning electron microscopy (SEM)	1177:1210	Scanning electron microscopy (SEM) reveals that cellulose fibers are visibly embedded into the inner hydrogel structure.					
37108723	8	20	theme	Scanning	1177:1184	arg1	SEM					1207:1209	SEM	1207:1209	SEM	1207:1209	Scanning electron microscopy (SEM) reveals that cellulose fibers are visibly embedded into the inner hydrogel structure.					
37108723	2	21	theme	inexpensive	384:394	arg1	systems					396:402	sustainable and inexpensive systems	368:402	sustainable and inexpensive systems	368:402	The removal of these dyes opens up new prospects for environmental remediation, but the design of sustainable and inexpensive systems for water purification is a fundamental challenge.					
37108723	5	22	with	cellobiose	870:879	arg1	moieties					1008:1015	polymerizable/crosslinkable moieties	980:1015	polymerizable/crosslinkable moieties	980:1015	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	2	23	theme	fundamental	432:442	arg1	challenge					444:452	a fundamental challenge	430:452	a fundamental challenge	430:452	The removal of these dyes opens up new prospects for environmental remediation, but the design of sustainable and inexpensive systems for water purification is a fundamental challenge.					
37108723	2	23	theme	fundamental	432:442	arg1	design					358:363	the design	354:363	the design of sustainable and inexpensive systems for water purification	354:425	The removal of these dyes opens up new prospects for environmental remediation, but the design of sustainable and inexpensive systems for water purification is a fundamental challenge.					
37108723	6	24	theme	good	1047:1050	arg1	yields					1077:1082	good (75%) to excellent (96%) yields	1047:1082	good (75%) to excellent (96%) yields	1047:1082	The networks are formed with good (75%) to excellent (96%) yields.					
37108723	1	25	theme	organic	173:179	arg1	dyes					181:184	organic dyes	173:184	organic dyes	173:184	Pollution with organic dyes is one of the most typical environmental problems related to industrial wastewater.					
37108723	9	26	theme	crystal	1400:1406	arg1	CV					1416:1417	CV	1416:1417	CV	1416:1417	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	9	26	theme	crystal	1400:1406	arg1	violet					1408:1413	crystal violet	1400:1413	crystal violet (CV)	1400:1418	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	9	27	theme	safeguarding	1541:1552	arg1	water					1535:1539	clean water safeguarding	1529:1552	clean water safeguarding	1529:1552	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	2	28	theme	new	305:307	arg1	prospects					309:317	new prospects	305:317	new prospects for environmental remediation	305:347	The removal of these dyes opens up new prospects for environmental remediation, but the design of sustainable and inexpensive systems for water purification is a fundamental challenge.					
37108723	9	29	dep	ability	1302:1308	arg1	bind					1313:1316	bind	1313:1316	to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions	1310:1442	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	2	30	theme	sustainable	368:378	arg1	systems					396:402	sustainable and inexpensive systems	368:402	sustainable and inexpensive systems	368:402	The removal of these dyes opens up new prospects for environmental remediation, but the design of sustainable and inexpensive systems for water purification is a fundamental challenge.					
37108723	0	31	theme	Composite	0:8	arg1	Hydrogels					10:18	Composite Hydrogels	0:18	Composite Hydrogels	0:18	Composite Hydrogels Based on Poly(Ethylene Glycol) and Cellulose Macromonomers as Fortified Materials for Environmental Cleanup and Clean Water Safeguarding.					
37108723	9	32	theme	aqueous	1426:1432	arg1	solutions					1434:1442	aqueous solutions	1426:1442	aqueous solutions	1426:1442	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	8	33	theme	electron	1186:1193	arg1	microscopy					1195:1204	Scanning electron microscopy	1177:1204	Scanning electron microscopy (SEM)	1177:1210	Scanning electron microscopy (SEM) reveals that cellulose fibers are visibly embedded into the inner hydrogel structure.					
37108723	8	33	theme	electron	1186:1193	arg1	SEM					1207:1209	SEM	1207:1209	SEM	1207:1209	Scanning electron microscopy (SEM) reveals that cellulose fibers are visibly embedded into the inner hydrogel structure.					
37108723	9	34	theme	environmental	1503:1515	arg1	cleanup					1517:1523	environmental cleanup	1503:1523	environmental cleanup	1503:1523	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	5	35	used	used	794:797	arg2	etherification					740:753	Williamson etherification	729:753	Williamson etherification with 4-vinylbenzyl chloride (4-VBC)	729:789	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	5	36	theme	different	817:825	arg1	masses					837:842	different molecular masses	817:842	different molecular masses (1, 5, 6, and 10 kDa)	817:864	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	5	36	theme	different	817:825	arg1	kDa					861:863	1, 5, 6, and 10 kDa	845:863	1, 5, 6, and 10 kDa	845:863	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	0	37	theme	Environmental	106:118	arg1	Cleanup					120:126	Environmental Cleanup	106:126	Environmental Cleanup	106:126	Composite Hydrogels Based on Poly(Ethylene Glycol) and Cellulose Macromonomers as Fortified Materials for Environmental Cleanup and Clean Water Safeguarding.					
37108723	2	38	theme	dyes	291:294	arg1	removal					274:280	The removal	270:280	The removal of these dyes	270:294	The removal of these dyes opens up new prospects for environmental remediation, but the design of sustainable and inexpensive systems for water purification is a fundamental challenge.					
37108723	2	39	theme	water	408:412	arg1	purification					414:425	water purification	408:425	water purification	408:425	The removal of these dyes opens up new prospects for environmental remediation, but the design of sustainable and inexpensive systems for water purification is a fundamental challenge.					
37108723	5	40	with	cellulose	913:921	arg1	moieties					1008:1015	polymerizable/crosslinkable moieties	980:1015	polymerizable/crosslinkable moieties	980:1015	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	3	41	theme	novel	491:495	arg1	hydrogels					507:515	novel fortified hydrogels	491:515	novel fortified hydrogels that can bind and remove organic dyes from aqueous solutions	491:576	This paper reports the synthesis of novel fortified hydrogels that can bind and remove organic dyes from aqueous solutions.					
37108723	0	42	theme	Clean	132:136	arg1	Water					138:142	Clean Water	132:142	Clean Water	132:142	Composite Hydrogels Based on Poly(Ethylene Glycol) and Cellulose Macromonomers as Fortified Materials for Environmental Cleanup and Clean Water Safeguarding.					
37108723	7	43	theme	good	1095:1098	arg1	swelling					1100:1107	good swelling	1095:1107	good swelling	1095:1107	They show good swelling and have good mechanical properties according to rheological tests.					
37108723	5	44	theme	renewable	954:962	arg1	resources					964:972	natural renewable resources	946:972	natural renewable resources	946:972	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	5	45	theme	masses	837:842	arg1	cellulose					913:921	Technocell™ T-90 cellulose	896:921	Technocell™ T-90 cellulose (products derived from natural renewable resources)	896:973	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	5	45	theme	masses	837:842	arg1	PEGs					809:812	PEGs	809:812	PEGs of different molecular masses (1, 5, 6, and 10 kDa)	809:864	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	5	45	theme	masses	837:842	arg1	cellobiose					870:879	cellobiose	870:879	cellobiose	870:879	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	0	46	theme	Poly	29:32	arg1	Macromonomers					65:77	Poly(Ethylene Glycol) and Cellulose Macromonomers	29:77	Poly(Ethylene Glycol) and Cellulose Macromonomers as Fortified Materials for Environmental Cleanup and Clean Water Safeguarding	29:155	Composite Hydrogels Based on Poly(Ethylene Glycol) and Cellulose Macromonomers as Fortified Materials for Environmental Cleanup and Clean Water Safeguarding.					
37108723	7	47	contain	have	1113:1116	arg1	They					1085:1088	They	1085:1088	They	1085:1088	They show good swelling and have good mechanical properties according to rheological tests.					
37108723	7	47	contain	have	1113:1116	arg2	properties					1134:1143	good mechanical properties	1118:1143	good mechanical properties according to rheological tests	1118:1174	They show good swelling and have good mechanical properties according to rheological tests.					
37108723	4	48	theme	modified	630:637	arg1	poly					639:642	chemically modified poly	619:642	chemically modified poly(ethylene glycol) (PEG-m)	619:667	These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers ("cellu-mers").					
37108723	4	48	theme	modified	630:637	arg1	glycol					653:658	ethylene glycol	644:658	ethylene glycol	644:658	These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers ("cellu-mers").					
37108723	4	48	theme	modified	630:637	arg1	PEG-m					662:666	PEG-m	662:666	PEG-m	662:666	These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers ("cellu-mers").					
37108723	9	49	theme	organic	1329:1335	arg1	dyes					1337:1340	organic dyes	1329:1340	organic dyes	1329:1340	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	9	49	theme	organic	1329:1335	arg1	violet					1408:1413	crystal violet	1400:1413	crystal violet (CV)	1400:1418	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	9	49	theme	organic	1329:1335	arg1	bromophenol					1351:1361	bromophenol blue	1351:1366	bromophenol blue (BPB)	1351:1372	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	9	49	theme	organic	1329:1335	arg1	methylene					1375:1383	methylene blue	1375:1388	methylene blue (MB)	1375:1393	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	2	50	theme	systems	396:402	arg1	challenge					444:452	a fundamental challenge	430:452	a fundamental challenge	430:452	The removal of these dyes opens up new prospects for environmental remediation, but the design of sustainable and inexpensive systems for water purification is a fundamental challenge.					
37108723	2	50	theme	systems	396:402	arg1	design					358:363	the design	354:363	the design of sustainable and inexpensive systems for water purification	354:425	The removal of these dyes opens up new prospects for environmental remediation, but the design of sustainable and inexpensive systems for water purification is a fundamental challenge.					
37108723	9	51	dep	bind	1313:1316	arg1	remove					1322:1327	remove	1322:1327	remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV)	1322:1418	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	5	52	theme	T-90	908:911	arg1	cellulose					913:921	Technocell™ T-90 cellulose	896:921	Technocell™ T-90 cellulose (products derived from natural renewable resources)	896:973	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	5	53	dep	cellulose	913:921	arg1	products					924:931	products	924:931	products derived from natural renewable resources	924:972	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	0	54	theme	Ethylene	34:41	arg1	Poly					29:32	Poly	29:32	Poly(Ethylene Glycol)	29:49	Composite Hydrogels Based on Poly(Ethylene Glycol) and Cellulose Macromonomers as Fortified Materials for Environmental Cleanup and Clean Water Safeguarding.					
37108723	0	54	theme	Ethylene	34:41	arg1	Glycol					43:48	Ethylene Glycol	34:48	Ethylene Glycol	34:48	Composite Hydrogels Based on Poly(Ethylene Glycol) and Cellulose Macromonomers as Fortified Materials for Environmental Cleanup and Clean Water Safeguarding.					
37108723	5	55	theme	polymerizable/crosslinkable	980:1006	arg1	moieties					1008:1015	polymerizable/crosslinkable moieties	980:1015	polymerizable/crosslinkable moieties	980:1015	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	7	56	theme	good	1118:1121	arg1	properties					1134:1143	good mechanical properties	1118:1143	good mechanical properties according to rheological tests	1118:1174	They show good swelling and have good mechanical properties according to rheological tests.					
37108723	2	57	theme	environmental	323:335	arg1	remediation					337:347	environmental remediation	323:347	environmental remediation	323:347	The removal of these dyes opens up new prospects for environmental remediation, but the design of sustainable and inexpensive systems for water purification is a fundamental challenge.					
37108723	9	58	theme	hydrogels	1489:1497	arg1	potential					1457:1465	the potential	1453:1465	the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding	1453:1552	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	5	59	with	etherification	740:753	arg1	4-VBC					784:788	4-VBC	784:788	4-VBC	784:788	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	5	59	with	etherification	740:753	arg1	chloride					774:781	4-vinylbenzyl chloride	760:781	4-vinylbenzyl chloride (4-VBC)	760:789	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	8	60	theme	cellulose	1225:1233	arg1	fibers					1235:1240	cellulose fibers	1225:1240	cellulose fibers	1225:1240	Scanning electron microscopy (SEM) reveals that cellulose fibers are visibly embedded into the inner hydrogel structure.					
37108723	8	61	theme	inner	1272:1276	arg1	structure					1287:1295	the inner hydrogel structure	1268:1295	the inner hydrogel structure	1268:1295	Scanning electron microscopy (SEM) reveals that cellulose fibers are visibly embedded into the inner hydrogel structure.					
37108723	1	62	theme	typical	205:211	arg1	problems					227:234	the most typical environmental problems	196:234	the most typical environmental problems related to industrial wastewater	196:267	Pollution with organic dyes is one of the most typical environmental problems related to industrial wastewater.					
37108723	5	63	with	PEGs	809:812	arg1	moieties					1008:1015	polymerizable/crosslinkable moieties	980:1015	polymerizable/crosslinkable moieties	980:1015	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	9	64	theme	new	1474:1476	arg1	hydrogels					1489:1497	the new cellulosic hydrogels	1470:1497	the new cellulosic hydrogels for environmental cleanup and clean water safeguarding	1470:1552	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	4	65	theme	cellu-mers	715:724	arg1	macromonomers					699:711	multifunctional cellulose macromonomers	673:711	multifunctional cellulose macromonomers ("cellu-mers")	673:726	These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers ("cellu-mers").					
37108723	4	65	theme	cellu-mers	715:724	arg1	"					725:725	"cellu-mers"	714:725	"cellu-mers"	714:725	These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers ("cellu-mers").					
37108723	1	66	theme	environmental	213:225	arg1	problems					227:234	the most typical environmental problems	196:234	the most typical environmental problems related to industrial wastewater	196:267	Pollution with organic dyes is one of the most typical environmental problems related to industrial wastewater.					
37108723	4	67	theme	ethylene	644:651	arg1	poly					639:642	chemically modified poly	619:642	chemically modified poly(ethylene glycol) (PEG-m)	619:667	These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers ("cellu-mers").					
37108723	4	67	theme	ethylene	644:651	arg1	glycol					653:658	ethylene glycol	644:658	ethylene glycol	644:658	These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers ("cellu-mers").					
37108723	9	68	theme	cellulosic	1478:1487	arg1	hydrogels					1489:1497	the new cellulosic hydrogels	1470:1497	the new cellulosic hydrogels for environmental cleanup and clean water safeguarding	1470:1552	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	4	69	theme	multifunctional	673:687	arg1	macromonomers					699:711	multifunctional cellulose macromonomers	673:711	multifunctional cellulose macromonomers ("cellu-mers")	673:726	These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers ("cellu-mers").					
37108723	4	69	theme	multifunctional	673:687	arg1	"					725:725	"cellu-mers"	714:725	"cellu-mers"	714:725	These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers ("cellu-mers").					
37108723	3	70	from	solutions	568:576	arg1	dyes					550:553	organic dyes	542:553	organic dyes from aqueous solutions	542:576	This paper reports the synthesis of novel fortified hydrogels that can bind and remove organic dyes from aqueous solutions.					
37108723	1	71	theme	problems	227:234	arg1	problems					227:234	the most typical environmental problems	196:234	the most typical environmental problems related to industrial wastewater	196:267	Pollution with organic dyes is one of the most typical environmental problems related to industrial wastewater.					
37108723	1	71	theme	problems	227:234	arg1	one					189:191	one	189:191	one	189:191	Pollution with organic dyes is one of the most typical environmental problems related to industrial wastewater.					
37108723	5	72	theme	4-vinylbenzyl	760:772	arg1	4-VBC					784:788	4-VBC	784:788	4-VBC	784:788	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	5	72	theme	4-vinylbenzyl	760:772	arg1	chloride					774:781	4-vinylbenzyl chloride	760:781	4-vinylbenzyl chloride (4-VBC)	760:789	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	9	73	theme	blue	1363:1366	arg1	bromophenol					1351:1361	bromophenol blue	1351:1366	bromophenol blue (BPB)	1351:1372	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	9	73	theme	blue	1363:1366	arg1	BPB					1369:1371	BPB	1369:1371	BPB	1369:1371	The ability to bind and remove organic dyes, such as bromophenol blue (BPB), methylene blue (MB), and crystal violet (CV), from aqueous solutions hints at the potential of the new cellulosic hydrogels for environmental cleanup and clean water safeguarding.					
37108723	5	74	theme	natural	946:952	arg1	resources					964:972	natural renewable resources	946:972	natural renewable resources	946:972	Williamson etherification with 4-vinylbenzyl chloride (4-VBC) is used to modify PEGs of different molecular masses (1, 5, 6, and 10 kDa) and cellobiose, Sigmacell, or Technocell™ T-90 cellulose (products derived from natural renewable resources) with polymerizable/crosslinkable moieties.					
37108723	7	75	theme	rheological	1158:1168	arg1	tests					1170:1174	rheological tests	1158:1174	rheological tests	1158:1174	They show good swelling and have good mechanical properties according to rheological tests.					
37108723	1	76	theme	related	236:242	arg1	problems					227:234	the most typical environmental problems	196:234	the most typical environmental problems related to industrial wastewater	196:267	Pollution with organic dyes is one of the most typical environmental problems related to industrial wastewater.					
37094645	1	0	theme	drug	177:180	arg1	system					191:196	an efficient drug delivery system	164:196	an efficient drug delivery system to mitigate the harm caused by root-knot nematodes	164:247	Developing an efficient drug delivery system to mitigate the harm caused by root-knot nematodes is crucial.					
37094645	5	1	theme	root-knot	742:750	arg1	nematodes					752:760	root-knot nematodes	742:760	root-knot nematodes	742:760	Moreover, AVB1a NCs improved the permeability of AVB1a to root-knot nematodes and plant roots and the horizontal and vertical soil mobility.					
37094645	6	2	theme	AVB1a	882:886	arg1	adsorption					868:877	the adsorption	864:877	the adsorption of AVB1a by the soil	864:898	Furthermore, AVB1a NCs greatly reduced the adsorption of AVB1a by the soil compared to AVB1a emulsifiable concentrate (EC), and the effect of the AVB1a NCs on controlling root-knot nematode disease was increased by 36 %.					
37094645	7	3	theme	AVB1a	1062:1066	arg1	EC					1068:1069	the AVB1a EC	1058:1069	the AVB1a EC	1058:1069	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	1	4	theme	delivery	182:189	arg1	system					191:196	an efficient drug delivery system	164:196	an efficient drug delivery system to mitigate the harm caused by root-knot nematodes	164:247	Developing an efficient drug delivery system to mitigate the harm caused by root-knot nematodes is crucial.					
37094645	8	5	theme	pesticide	1310:1318	arg1	system					1329:1334	This enzyme-responsive pesticide delivery system	1287:1334	This enzyme-responsive pesticide delivery system	1287:1334	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	8	6	theme	preparation	1349:1359	arg1	method					1361:1366	a simple preparation method	1340:1366	a simple preparation method	1340:1366	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	8	7	theme	simple	1342:1347	arg1	method					1361:1366	a simple preparation method	1340:1366	a simple preparation method	1340:1366	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	7	8	dep	times	1197:1201	arg1	that					1203:1206	that	1203:1206	that	1203:1206	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	0	9	theme	control	107:113	arg1	effect					115:120	the control effect	103:120	the control effect of root-knot nematode disease	103:150	Preparation of enzyme-responsive composite nanocapsules with sodium carboxymethyl cellulose to improve the control effect of root-knot nematode disease.					
37094645	2	10	theme	abamectin	302:310	arg1	nanocapsules					312:323	enzyme-responsive release abamectin nanocapsules	276:323	enzyme-responsive release abamectin nanocapsules (AVB1a NCs)	276:335	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	10	theme	abamectin	302:310	arg1	NCs					332:334	AVB1a NCs	326:334	AVB1a NCs	326:334	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	11	theme	4-diphenylmethane	360:376	arg1	cellulose					422:430	sodium carboxymethyl cellulose	401:430	sodium carboxymethyl cellulose	401:430	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	11	theme	4-diphenylmethane	360:376	arg1	MDI					392:394	MDI	392:394	MDI	392:394	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	11	theme	4-diphenylmethane	360:376	arg1	diisocyanate					378:389	4, 4-diphenylmethane diisocyanate	357:389	diisocyanate	378:389	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	11	theme	4-diphenylmethane	360:376	arg1	factors					452:458	response release factors	435:458	response release factors	435:458	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	0	12	theme	root-knot	125:133	arg1	disease					144:150	root-knot nematode disease	125:150	root-knot nematode disease	125:150	Preparation of enzyme-responsive composite nanocapsules with sodium carboxymethyl cellulose to improve the control effect of root-knot nematode disease.					
37094645	8	13	contain	had	1336:1338	arg2	performance					1379:1389	excellent performance	1369:1389	excellent performance	1369:1389	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	8	13	contain	had	1336:1338	arg1	system					1329:1334	This enzyme-responsive pesticide delivery system	1287:1334	This enzyme-responsive pesticide delivery system	1287:1334	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	8	13	contain	had	1336:1338	arg2	method					1361:1366	a simple preparation method	1340:1366	a simple preparation method	1340:1366	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	8	13	contain	had	1336:1338	arg2	level					1401:1405	high level	1396:1405	high level of safety	1396:1415	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	7	14	theme	lower	1231:1235	arg1	impact					1245:1250	a lower overall impact	1229:1250	a lower overall impact	1229:1250	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	2	15	theme	release	294:300	arg1	nanocapsules					312:323	enzyme-responsive release abamectin nanocapsules	276:323	enzyme-responsive release abamectin nanocapsules (AVB1a NCs)	276:335	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	15	theme	release	294:300	arg1	NCs					332:334	AVB1a NCs	326:334	AVB1a NCs	326:334	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	7	16	theme	overall	1237:1243	arg1	impact					1245:1250	a lower overall impact	1229:1250	a lower overall impact	1229:1250	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	2	17	theme	sodium	401:406	arg1	cellulose					422:430	sodium carboxymethyl cellulose	401:430	sodium carboxymethyl cellulose	401:430	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	17	theme	sodium	401:406	arg1	diisocyanate					378:389	4, 4-diphenylmethane diisocyanate	357:389	diisocyanate	378:389	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	17	theme	sodium	401:406	arg1	factors					452:458	response release factors	435:458	response release factors	435:458	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	7	18	theme	acute	1128:1132	arg1	toxicity					1134:1141	the acute toxicity	1124:1141	the acute toxicity to the soil biological earthworms	1124:1175	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	8	19	theme	plant	1463:1467	arg1	diseases					1469:1476	plant diseases	1463:1476	plant diseases	1463:1476	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	2	20	theme	enzyme-responsive	276:292	arg1	nanocapsules					312:323	enzyme-responsive release abamectin nanocapsules	276:323	enzyme-responsive release abamectin nanocapsules (AVB1a NCs)	276:335	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	20	theme	enzyme-responsive	276:292	arg1	NCs					332:334	AVB1a NCs	326:334	AVB1a NCs	326:334	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	4	21	theme	AVB1a	622:626	arg1	NCs					628:630	AVB1a NCs	622:630	AVB1a NCs	622:630	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity was 0.82 mg L-1.					
37094645	7	22	theme	soil	1150:1153	arg1	earthworms					1166:1175	the soil biological earthworms	1146:1175	the soil biological earthworms	1146:1175	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	3	23	theme	encapsulation	545:557	arg1	%					577:577	92 %	574:577	92 %	574:577	The results showed that the average size (D50) of the AVB1a NCs was 352 nm, and the encapsulation efficiency was 92 %.					
37094645	3	23	theme	encapsulation	545:557	arg1	efficiency					559:568	the encapsulation efficiency	541:568	the encapsulation efficiency	541:568	The results showed that the average size (D50) of the AVB1a NCs was 352 nm, and the encapsulation efficiency was 92 %.					
37094645	3	24	theme	NCs	521:523	arg1	352 nm					529:534	352 nm	529:534	352 nm	529:534	The results showed that the average size (D50) of the AVB1a NCs was 352 nm, and the encapsulation efficiency was 92 %.					
37094645	3	24	theme	NCs	521:523	arg1	D50					503:505	D50	503:505	D50	503:505	The results showed that the average size (D50) of the AVB1a NCs was 352 nm, and the encapsulation efficiency was 92 %.					
37094645	3	24	theme	NCs	521:523	arg1	size					497:500	the average size	485:500	the average size (D50) of the AVB1a NCs	485:523	The results showed that the average size (D50) of the AVB1a NCs was 352 nm, and the encapsulation efficiency was 92 %.					
37094645	4	25	theme	Meloidogyne	636:646	arg1	activity					658:665	Meloidogyne incognita activity	636:665	Meloidogyne incognita activity	636:665	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity was 0.82 mg L-1.					
37094645	6	26	theme	AVB1a	971:975	arg1	NCs					977:979	the AVB1a NCs	967:979	the AVB1a NCs	967:979	Furthermore, AVB1a NCs greatly reduced the adsorption of AVB1a by the soil compared to AVB1a emulsifiable concentrate (EC), and the effect of the AVB1a NCs on controlling root-knot nematode disease was increased by 36 %.					
37094645	7	27	theme	pesticide	1076:1084	arg1	system					1095:1100	the pesticide delivery system	1072:1100	the pesticide delivery system	1072:1100	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	8	28	theme	safety	1410:1415	arg1	method					1361:1366	a simple preparation method	1340:1366	a simple preparation method	1340:1366	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	8	28	theme	safety	1410:1415	arg1	performance					1379:1389	excellent performance	1369:1389	excellent performance	1369:1389	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	8	28	theme	safety	1410:1415	arg1	level					1401:1405	high level	1396:1405	high level of safety	1396:1415	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	8	29	contain	has	1427:1429	arg1	system					1329:1334	This enzyme-responsive pesticide delivery system	1287:1334	This enzyme-responsive pesticide delivery system	1287:1334	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	8	29	contain	has	1427:1429	arg2	pests					1489:1493	insect pests	1482:1493	insect pests	1482:1493	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	8	29	contain	has	1427:1429	arg2	potential					1449:1457	great application potential	1431:1457	great application potential for plant diseases	1431:1476	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	6	30	theme	nematode	1006:1013	arg1	disease					1015:1021	root-knot nematode disease	996:1021	root-knot nematode disease	996:1021	Furthermore, AVB1a NCs greatly reduced the adsorption of AVB1a by the soil compared to AVB1a emulsifiable concentrate (EC), and the effect of the AVB1a NCs on controlling root-knot nematode disease was increased by 36 %.					
37094645	0	31	theme	composite	33:41	arg1	Preparation					0:10	Preparation	0:10	Preparation of enzyme-responsive composite	0:41	Preparation of enzyme-responsive composite nanocapsules with sodium carboxymethyl cellulose to improve the control effect of root-knot nematode disease.					
37094645	8	32	theme	excellent	1369:1377	arg1	performance					1379:1389	excellent performance	1369:1389	excellent performance	1369:1389	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	0	33	theme	disease	144:150	arg1	effect					115:120	the control effect	103:120	the control effect of root-knot nematode disease	103:150	Preparation of enzyme-responsive composite nanocapsules with sodium carboxymethyl cellulose to improve the control effect of root-knot nematode disease.					
37094645	7	34	theme	microbial	1264:1272	arg1	communities					1274:1284	the soil microbial communities	1255:1284	the soil microbial communities	1255:1284	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	2	35	theme	AVB1a	326:330	arg1	nanocapsules					312:323	enzyme-responsive release abamectin nanocapsules	276:323	enzyme-responsive release abamectin nanocapsules (AVB1a NCs)	276:335	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	35	theme	AVB1a	326:330	arg1	NCs					332:334	AVB1a NCs	326:334	AVB1a NCs	326:334	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	36	theme	response	435:442	arg1	cellulose					422:430	sodium carboxymethyl cellulose	401:430	sodium carboxymethyl cellulose	401:430	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	36	theme	response	435:442	arg1	diisocyanate					378:389	4, 4-diphenylmethane diisocyanate	357:389	diisocyanate	378:389	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	36	theme	response	435:442	arg1	factors					452:458	response release factors	435:458	response release factors	435:458	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	37	theme	release	444:450	arg1	cellulose					422:430	sodium carboxymethyl cellulose	401:430	sodium carboxymethyl cellulose	401:430	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	37	theme	release	444:450	arg1	diisocyanate					378:389	4, 4-diphenylmethane diisocyanate	357:389	diisocyanate	378:389	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	37	theme	release	444:450	arg1	factors					452:458	response release factors	435:458	response release factors	435:458	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	8	38	theme	application	1437:1447	arg1	potential					1449:1457	great application potential	1431:1457	great application potential for plant diseases	1431:1476	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	0	39	theme	nematode	135:142	arg1	disease					144:150	root-knot nematode disease	125:150	root-knot nematode disease	125:150	Preparation of enzyme-responsive composite nanocapsules with sodium carboxymethyl cellulose to improve the control effect of root-knot nematode disease.					
37094645	7	40	contain	had	1225:1227	arg1	system					1095:1100	the pesticide delivery system	1072:1100	the pesticide delivery system	1072:1100	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	7	40	contain	had	1225:1227	arg2	impact					1245:1250	a lower overall impact	1229:1250	a lower overall impact	1229:1250	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	0	41	dep	cellulose	82:90	arg1	improve					95:101	improve	95:101	to improve the control effect of root-knot nematode disease	92:150	Preparation of enzyme-responsive composite nanocapsules with sodium carboxymethyl cellulose to improve the control effect of root-knot nematode disease.					
37094645	8	42	theme	enzyme-responsive	1292:1308	arg1	system					1329:1334	This enzyme-responsive pesticide delivery system	1287:1334	This enzyme-responsive pesticide delivery system	1287:1334	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	5	43	theme	soil	810:813	arg1	mobility					815:822	the horizontal and vertical soil mobility	782:822	the horizontal and vertical soil mobility	782:822	Moreover, AVB1a NCs improved the permeability of AVB1a to root-knot nematodes and plant roots and the horizontal and vertical soil mobility.					
37094645	6	44	theme	emulsifiable	918:929	arg1	concentrate					931:941	AVB1a emulsifiable concentrate	912:941	AVB1a emulsifiable concentrate (EC)	912:946	Furthermore, AVB1a NCs greatly reduced the adsorption of AVB1a by the soil compared to AVB1a emulsifiable concentrate (EC), and the effect of the AVB1a NCs on controlling root-knot nematode disease was increased by 36 %.					
37094645	6	44	theme	emulsifiable	918:929	arg1	EC					944:945	EC	944:945	EC	944:945	Furthermore, AVB1a NCs greatly reduced the adsorption of AVB1a by the soil compared to AVB1a emulsifiable concentrate (EC), and the effect of the AVB1a NCs on controlling root-knot nematode disease was increased by 36 %.					
37094645	6	45	theme	AVB1a	838:842	arg1	NCs					844:846	AVB1a NCs	838:846	AVB1a NCs	838:846	Furthermore, AVB1a NCs greatly reduced the adsorption of AVB1a by the soil compared to AVB1a emulsifiable concentrate (EC), and the effect of the AVB1a NCs on controlling root-knot nematode disease was increased by 36 %.					
37094645	4	46	theme	NCs	628:630	arg1	0.82 mg L-1					671:681	0.82 mg L-1	671:681	0.82 mg L-1	671:681	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity was 0.82 mg L-1.					
37094645	4	46	theme	NCs	628:630	arg1	LC50					613:616	LC50	613:616	LC50	613:616	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity was 0.82 mg L-1.					
37094645	4	46	theme	NCs	628:630	arg1	concentration					598:610	The median lethal concentration	580:610	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity	580:665	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity was 0.82 mg L-1.					
37094645	6	47	theme	AVB1a	912:916	arg1	concentrate					931:941	AVB1a emulsifiable concentrate	912:941	AVB1a emulsifiable concentrate (EC)	912:946	Furthermore, AVB1a NCs greatly reduced the adsorption of AVB1a by the soil compared to AVB1a emulsifiable concentrate (EC), and the effect of the AVB1a NCs on controlling root-knot nematode disease was increased by 36 %.					
37094645	6	47	theme	AVB1a	912:916	arg1	EC					944:945	EC	944:945	EC	944:945	Furthermore, AVB1a NCs greatly reduced the adsorption of AVB1a by the soil compared to AVB1a emulsifiable concentrate (EC), and the effect of the AVB1a NCs on controlling root-knot nematode disease was increased by 36 %.					
37094645	5	48	theme	AVB1a	694:698	arg1	NCs					700:702	AVB1a NCs	694:702	AVB1a NCs	694:702	Moreover, AVB1a NCs improved the permeability of AVB1a to root-knot nematodes and plant roots and the horizontal and vertical soil mobility.					
37094645	8	49	theme	high	1396:1399	arg1	level					1401:1405	high level	1396:1405	high level of safety	1396:1415	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	5	50	theme	horizontal	786:795	arg1	mobility					815:822	the horizontal and vertical soil mobility	782:822	the horizontal and vertical soil mobility	782:822	Moreover, AVB1a NCs improved the permeability of AVB1a to root-knot nematodes and plant roots and the horizontal and vertical soil mobility.					
37094645	2	51	theme	carboxymethyl	408:420	arg1	cellulose					422:430	sodium carboxymethyl cellulose	401:430	sodium carboxymethyl cellulose	401:430	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	51	theme	carboxymethyl	408:420	arg1	diisocyanate					378:389	4, 4-diphenylmethane diisocyanate	357:389	diisocyanate	378:389	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	2	51	theme	carboxymethyl	408:420	arg1	factors					452:458	response release factors	435:458	response release factors	435:458	In this study, enzyme-responsive release abamectin nanocapsules (AVB1a NCs) were prepared using 4, 4-diphenylmethane diisocyanate (MDI) and sodium carboxymethyl cellulose as response release factors.					
37094645	0	52	theme	carboxymethyl	68:80	arg1	cellulose					82:90	sodium carboxymethyl cellulose	61:90	sodium carboxymethyl cellulose to improve the control effect of root-knot nematode disease	61:150	Preparation of enzyme-responsive composite nanocapsules with sodium carboxymethyl cellulose to improve the control effect of root-knot nematode disease.					
37094645	6	53	theme	NCs	977:979	arg1	effect					957:962	the effect	953:962	the effect of the AVB1a NCs on controlling root-knot nematode disease	953:1021	Furthermore, AVB1a NCs greatly reduced the adsorption of AVB1a by the soil compared to AVB1a emulsifiable concentrate (EC), and the effect of the AVB1a NCs on controlling root-knot nematode disease was increased by 36 %.					
37094645	5	54	theme	AVB1a	733:737	arg1	mobility					815:822	the horizontal and vertical soil mobility	782:822	the horizontal and vertical soil mobility	782:822	Moreover, AVB1a NCs improved the permeability of AVB1a to root-knot nematodes and plant roots and the horizontal and vertical soil mobility.					
37094645	5	54	theme	AVB1a	733:737	arg1	permeability					717:728	the permeability	713:728	the permeability of AVB1a to root-knot nematodes and plant roots	713:776	Moreover, AVB1a NCs improved the permeability of AVB1a to root-knot nematodes and plant roots and the horizontal and vertical soil mobility.					
37094645	1	55	dep	system	191:196	arg1	mitigate					201:208	mitigate	201:208	to mitigate the harm caused by root-knot nematodes	198:247	Developing an efficient drug delivery system to mitigate the harm caused by root-knot nematodes is crucial.					
37094645	0	56	theme	sodium	61:66	arg1	cellulose					82:90	sodium carboxymethyl cellulose	61:90	sodium carboxymethyl cellulose to improve the control effect of root-knot nematode disease	61:150	Preparation of enzyme-responsive composite nanocapsules with sodium carboxymethyl cellulose to improve the control effect of root-knot nematode disease.					
37094645	8	57	theme	insect	1482:1487	arg1	pests					1489:1493	insect pests	1482:1493	insect pests	1482:1493	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	7	58	theme	AVB1a	1215:1219	arg1	times					1197:1201	approximately 16 times	1180:1201	approximately 16 times that of the AVB1a	1180:1219	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	7	59	theme	delivery	1086:1093	arg1	system					1095:1100	the pesticide delivery system	1072:1100	the pesticide delivery system	1072:1100	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	4	60	theme	incognita	648:656	arg1	activity					658:665	Meloidogyne incognita activity	636:665	Meloidogyne incognita activity	636:665	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity was 0.82 mg L-1.					
37094645	3	61	theme	average	489:495	arg1	352 nm					529:534	352 nm	529:534	352 nm	529:534	The results showed that the average size (D50) of the AVB1a NCs was 352 nm, and the encapsulation efficiency was 92 %.					
37094645	3	61	theme	average	489:495	arg1	D50					503:505	D50	503:505	D50	503:505	The results showed that the average size (D50) of the AVB1a NCs was 352 nm, and the encapsulation efficiency was 92 %.					
37094645	3	61	theme	average	489:495	arg1	size					497:500	the average size	485:500	the average size (D50) of the AVB1a NCs	485:523	The results showed that the average size (D50) of the AVB1a NCs was 352 nm, and the encapsulation efficiency was 92 %.					
37094645	8	62	theme	delivery	1320:1327	arg1	system					1329:1334	This enzyme-responsive pesticide delivery system	1287:1334	This enzyme-responsive pesticide delivery system	1287:1334	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	4	63	theme	median	584:589	arg1	0.82 mg L-1					671:681	0.82 mg L-1	671:681	0.82 mg L-1	671:681	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity was 0.82 mg L-1.					
37094645	4	63	theme	median	584:589	arg1	LC50					613:616	LC50	613:616	LC50	613:616	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity was 0.82 mg L-1.					
37094645	4	63	theme	median	584:589	arg1	concentration					598:610	The median lethal concentration	580:610	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity	580:665	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity was 0.82 mg L-1.					
37094645	6	64	theme	root-knot	996:1004	arg1	disease					1015:1021	root-knot nematode disease	996:1021	root-knot nematode disease	996:1021	Furthermore, AVB1a NCs greatly reduced the adsorption of AVB1a by the soil compared to AVB1a emulsifiable concentrate (EC), and the effect of the AVB1a NCs on controlling root-knot nematode disease was increased by 36 %.					
37094645	3	65	theme	AVB1a	515:519	arg1	NCs					521:523	the AVB1a NCs	511:523	the AVB1a NCs	511:523	The results showed that the average size (D50) of the AVB1a NCs was 352 nm, and the encapsulation efficiency was 92 %.					
37094645	7	66	theme	biological	1155:1164	arg1	earthworms					1166:1175	the soil biological earthworms	1146:1175	the soil biological earthworms	1146:1175	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	1	67	theme	root-knot	229:237	arg1	nematodes					239:247	root-knot nematodes	229:247	root-knot nematodes	229:247	Developing an efficient drug delivery system to mitigate the harm caused by root-knot nematodes is crucial.					
37094645	7	68	theme	soil	1259:1262	arg1	communities					1274:1284	the soil microbial communities	1255:1284	the soil microbial communities	1255:1284	Compared to the AVB1a EC, the pesticide delivery system significantly reduced the acute toxicity to the soil biological earthworms by approximately 16 times that of the AVB1a and had a lower overall impact on the soil microbial communities.					
37094645	5	69	theme	vertical	801:808	arg1	mobility					815:822	the horizontal and vertical soil mobility	782:822	the horizontal and vertical soil mobility	782:822	Moreover, AVB1a NCs improved the permeability of AVB1a to root-knot nematodes and plant roots and the horizontal and vertical soil mobility.					
37094645	8	70	dep	potential	1449:1457	arg1	control					1495:1501	control	1495:1501	control	1495:1501	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	5	71	theme	plant	766:770	arg1	roots					772:776	plant roots	766:776	plant roots	766:776	Moreover, AVB1a NCs improved the permeability of AVB1a to root-knot nematodes and plant roots and the horizontal and vertical soil mobility.					
37094645	0	72	theme	enzyme-responsive	15:31	arg1	composite					33:41	enzyme-responsive composite	15:41	enzyme-responsive composite	15:41	Preparation of enzyme-responsive composite nanocapsules with sodium carboxymethyl cellulose to improve the control effect of root-knot nematode disease.					
37094645	1	73	theme	efficient	167:175	arg1	system					191:196	an efficient drug delivery system	164:196	an efficient drug delivery system to mitigate the harm caused by root-knot nematodes	164:247	Developing an efficient drug delivery system to mitigate the harm caused by root-knot nematodes is crucial.					
37094645	8	74	theme	great	1431:1435	arg1	potential					1449:1457	great application potential	1431:1457	great application potential for plant diseases	1431:1476	This enzyme-responsive pesticide delivery system had a simple preparation method, excellent performance, and high level of safety, and thus has great application potential for plant diseases and insect pests control.					
37094645	4	75	theme	lethal	591:596	arg1	0.82 mg L-1					671:681	0.82 mg L-1	671:681	0.82 mg L-1	671:681	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity was 0.82 mg L-1.					
37094645	4	75	theme	lethal	591:596	arg1	LC50					613:616	LC50	613:616	LC50	613:616	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity was 0.82 mg L-1.					
37094645	4	75	theme	lethal	591:596	arg1	concentration					598:610	The median lethal concentration	580:610	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity	580:665	The median lethal concentration (LC50) of AVB1a NCs for Meloidogyne incognita activity was 0.82 mg L-1.					
36076916	2	0	theme	tissue	366:371	arg1	applications					385:396	tissue engineering applications	366:396	tissue engineering applications	366:396	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a nanostructure scaffold for tissue engineering applications.					
36076916	4	1	theme	PCL/CS	648:653	arg1	scaffold					674:681	a PCL and PCL/CS nanofiber membrane scaffold	638:681	a PCL and PCL/CS nanofiber membrane scaffold	638:681	The results demonstrate that a PCL and PCL/CS nanofiber membrane scaffold could be prepared with a comparable fiber diameter (~300 nm) and porosity for cell culture.					
36076916	1	2	theme	epithelial	186:195	arg1	cells					197:201	specific epithelial cells	177:201	specific epithelial cells lining the serosal cavity and internal organs	177:247	Mesothelial cells are specific epithelial cells lining the serosal cavity and internal organs.					
36076916	1	2	theme	epithelial	186:195	arg1	cells					167:171	Mesothelial cells	155:171	Mesothelial cells	155:171	Mesothelial cells are specific epithelial cells lining the serosal cavity and internal organs.					
36076916	8	3	theme	expression	1201:1210	arg1	analysis					1212:1219	gene expression analysis	1196:1219	gene expression analysis	1196:1219	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	4	4	theme	membrane	665:672	arg1	scaffold					674:681	a PCL and PCL/CS nanofiber membrane scaffold	638:681	a PCL and PCL/CS nanofiber membrane scaffold	638:681	The results demonstrate that a PCL and PCL/CS nanofiber membrane scaffold could be prepared with a comparable fiber diameter (~300 nm) and porosity for cell culture.					
36076916	5	5	theme	scaffold	840:847	arg1	properties					822:831	the mechanical properties	807:831	the mechanical properties of the scaffold due to interference of PCL crystallinity in the nanofibers	807:906	Blending CS with PCL influenced the mechanical properties of the scaffold due to interference of PCL crystallinity in the nanofibers.					
36076916	5	6	with	CS	784:785	arg1	PCL					792:794	PCL	792:794	PCL	792:794	Blending CS with PCL influenced the mechanical properties of the scaffold due to interference of PCL crystallinity in the nanofibers.					
36076916	3	7	theme	CS	559:560	arg1	effect					549:554	the effect	545:554	the effect of CS on the cellular response of mesothelial cells	545:606	Therefore, this study aims to fabricate nanofibers from a polycaprolactone (PCL) and PCL/chitosan (CS) blend by electrospinning, and to elucidate the effect of CS on the cellular response of mesothelial cells.					
36076916	5	8	theme	mechanical	811:820	arg1	properties					822:831	the mechanical properties	807:831	the mechanical properties of the scaffold due to interference of PCL crystallinity in the nanofibers	807:906	Blending CS with PCL influenced the mechanical properties of the scaffold due to interference of PCL crystallinity in the nanofibers.					
36076916	8	9	theme	upregulated	1298:1308	arg1	expression					1310:1319	the upregulated expression	1294:1319	the upregulated expression of mesothelial marker genes	1294:1347	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	0	10	theme	Damaged	134:140	arg1	Mesothelium					142:152	Damaged Mesothelium	134:152	Damaged Mesothelium	134:152	Polycaprolactone/Chitosan Composite Nanofiber Membrane as a Preferred Scaffold for the Culture of Mesothelial Cells and the Repair of Damaged Mesothelium.					
36076916	3	11	theme	mesothelial	590:600	arg1	cells					602:606	mesothelial cells	590:606	mesothelial cells	590:606	Therefore, this study aims to fabricate nanofibers from a polycaprolactone (PCL) and PCL/chitosan (CS) blend by electrospinning, and to elucidate the effect of CS on the cellular response of mesothelial cells.					
36076916	7	12	theme	mesothelial	1037:1047	arg1	cells					1049:1053	The mesothelial cells	1033:1053	The mesothelial cells	1033:1053	The mesothelial cells maintain high viability in both nanofiber membranes, but PCL/CS provides better maintenance of cobblestone-like mesothelial morphology.					
36076916	8	13	theme	marker	1336:1341	arg1	genes					1343:1347	mesothelial marker genes	1324:1347	mesothelial marker genes	1324:1347	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	11	14	theme	mesothelium	1802:1812	arg1	layer					1814:1818	a mesothelium layer	1800:1818	a mesothelium layer similar to native mesothelium tissue	1800:1855	In contrast, a mesothelium layer similar to native mesothelium tissue could be obtained by implanting the cell/scaffold construct, based on hematoxylin and eosin (H&E) and immunohistochemical staining.					
36076916	4	15	theme	fiber	719:723	arg1	diameter					725:732	a comparable fiber diameter	706:732	a comparable fiber diameter (~300 nm)	706:742	The results demonstrate that a PCL and PCL/CS nanofiber membrane scaffold could be prepared with a comparable fiber diameter (~300 nm) and porosity for cell culture.					
36076916	4	15	theme	fiber	719:723	arg1	nm					740:741	~300 nm	735:741	~300 nm	735:741	The results demonstrate that a PCL and PCL/CS nanofiber membrane scaffold could be prepared with a comparable fiber diameter (~300 nm) and porosity for cell culture.					
36076916	7	16	theme	morphology	1179:1188	arg1	maintenance					1135:1145	better maintenance	1128:1145	better maintenance of cobblestone-like mesothelial morphology	1128:1188	The mesothelial cells maintain high viability in both nanofiber membranes, but PCL/CS provides better maintenance of cobblestone-like mesothelial morphology.					
36076916	1	17	theme	Mesothelial	155:165	arg1	cells					197:201	specific epithelial cells	177:201	specific epithelial cells lining the serosal cavity and internal organs	177:247	Mesothelial cells are specific epithelial cells lining the serosal cavity and internal organs.					
36076916	1	17	theme	Mesothelial	155:165	arg1	cells					167:171	Mesothelial cells	155:171	Mesothelial cells	155:171	Mesothelial cells are specific epithelial cells lining the serosal cavity and internal organs.					
36076916	6	18	theme	cell	1001:1004	arg1	rate					1017:1020	a ~6-times-higher cell attachment rate	983:1020	a ~6-times-higher cell attachment rate in PCL/CS	983:1030	However, CS substantially improves scaffold hydrophilicity and results in a ~6-times-higher cell attachment rate in PCL/CS.					
36076916	8	19	theme	enhanced	1357:1364	arg1	production					1366:1375	the enhanced production	1353:1375	the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype	1353:1472	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	11	20	theme	native	1831:1836	arg1	tissue					1850:1855	native mesothelium tissue	1831:1855	native mesothelium tissue	1831:1855	In contrast, a mesothelium layer similar to native mesothelium tissue could be obtained by implanting the cell/scaffold construct, based on hematoxylin and eosin (H&E) and immunohistochemical staining.					
36076916	5	21	theme	crystallinity	876:888	arg1	interference					856:867	interference	856:867	interference of PCL crystallinity in the nanofibers	856:906	Blending CS with PCL influenced the mechanical properties of the scaffold due to interference of PCL crystallinity in the nanofibers.					
36076916	3	22	from	effect	549:554	arg1	response					578:585	the cellular response	565:585	the cellular response of mesothelial cells	565:606	Therefore, this study aims to fabricate nanofibers from a polycaprolactone (PCL) and PCL/chitosan (CS) blend by electrospinning, and to elucidate the effect of CS on the cellular response of mesothelial cells.					
36076916	11	23	theme	similar	1820:1826	arg1	layer					1814:1818	a mesothelium layer	1800:1818	a mesothelium layer similar to native mesothelium tissue	1800:1855	In contrast, a mesothelium layer similar to native mesothelium tissue could be obtained by implanting the cell/scaffold construct, based on hematoxylin and eosin (H&E) and immunohistochemical staining.					
36076916	0	24	theme	Mesothelial	98:108	arg1	Cells					110:114	Mesothelial Cells	98:114	Mesothelial Cells	98:114	Polycaprolactone/Chitosan Composite Nanofiber Membrane as a Preferred Scaffold for the Culture of Mesothelial Cells and the Repair of Damaged Mesothelium.					
36076916	5	25	from	interference	856:867	arg1	nanofibers					897:906	the nanofibers	893:906	the nanofibers	893:906	Blending CS with PCL influenced the mechanical properties of the scaffold due to interference of PCL crystallinity in the nanofibers.					
36076916	4	26	theme	comparable	708:717	arg1	diameter					725:732	a comparable fiber diameter	706:732	a comparable fiber diameter (~300 nm)	706:742	The results demonstrate that a PCL and PCL/CS nanofiber membrane scaffold could be prepared with a comparable fiber diameter (~300 nm) and porosity for cell culture.					
36076916	4	26	theme	comparable	708:717	arg1	nm					740:741	~300 nm	735:741	~300 nm	735:741	The results demonstrate that a PCL and PCL/CS nanofiber membrane scaffold could be prepared with a comparable fiber diameter (~300 nm) and porosity for cell culture.					
36076916	8	27	theme	mesothelial	1384:1394	arg1	proteins					1402:1409	key mesothelial maker proteins	1380:1409	key mesothelial maker proteins	1380:1409	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	9	28	theme	allograft	1607:1615	arg1	cells					1629:1633	allograft mesothelial cells	1607:1633	allograft mesothelial cells for mesothelium reconstruction in rats	1607:1672	The PCL/CS scaffold was therefore chosen for the in vivo studies, which involved transplanting a cell/scaffold construct containing allograft mesothelial cells for mesothelium reconstruction in rats.					
36076916	8	29	theme	proteins	1402:1409	arg1	expression					1310:1319	the upregulated expression	1294:1319	the upregulated expression of mesothelial marker genes	1294:1347	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	8	29	theme	proteins	1402:1409	arg1	production					1366:1375	the enhanced production	1353:1375	the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype	1353:1472	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	10	30	theme	cells	1705:1709	arg1	absence					1682:1688	the absence	1678:1688	the absence of mesothelial cells	1678:1709	In the absence of mesothelial cells, the mesothelium wound covered with PCL/CS showed an inflammatory response.					
36076916	2	31	theme	few	263:265	arg1	studies					267:273	few studies	263:273	few studies	263:273	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a nanostructure scaffold for tissue engineering applications.					
36076916	0	32	theme	Nanofiber	36:44	arg1	Membrane					46:53	Nanofiber Membrane	36:53	Nanofiber Membrane	36:53	Polycaprolactone/Chitosan Composite Nanofiber Membrane as a Preferred Scaffold for the Culture of Mesothelial Cells and the Repair of Damaged Mesothelium.					
36076916	9	33	theme	mesothelium	1639:1649	arg1	reconstruction					1651:1664	mesothelium reconstruction	1639:1664	mesothelium reconstruction in rats	1639:1672	The PCL/CS scaffold was therefore chosen for the in vivo studies, which involved transplanting a cell/scaffold construct containing allograft mesothelial cells for mesothelium reconstruction in rats.					
36076916	9	34	from	reconstruction	1651:1664	arg1	rats					1669:1672	rats	1669:1672	rats	1669:1672	The PCL/CS scaffold was therefore chosen for the in vivo studies, which involved transplanting a cell/scaffold construct containing allograft mesothelial cells for mesothelium reconstruction in rats.					
36076916	1	35	dep	cells	197:201	arg1	lining					203:208	lining	203:208	lining	203:208	Mesothelial cells are specific epithelial cells lining the serosal cavity and internal organs.					
36076916	7	36	theme	cobblestone-like	1150:1165	arg1	morphology					1179:1188	cobblestone-like mesothelial morphology	1150:1188	cobblestone-like mesothelial morphology	1150:1188	The mesothelial cells maintain high viability in both nanofiber membranes, but PCL/CS provides better maintenance of cobblestone-like mesothelial morphology.					
36076916	10	37	theme	inflammatory	1764:1775	arg1	response					1777:1784	an inflammatory response	1761:1784	an inflammatory response	1761:1784	In the absence of mesothelial cells, the mesothelium wound covered with PCL/CS showed an inflammatory response.					
36076916	5	38	theme	Blending	775:782	arg1	CS					784:785	Blending CS	775:785	Blending CS with PCL	775:794	Blending CS with PCL influenced the mechanical properties of the scaffold due to interference of PCL crystallinity in the nanofibers.					
36076916	8	39	theme	mesothelial	1452:1462	arg1	phenotype					1464:1472	the mesothelial phenotype	1448:1472	the mesothelial phenotype	1448:1472	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	3	40	from	PCL/chitosan	484:495	arg1	nanofibers					439:448	nanofibers	439:448	nanofibers from a polycaprolactone (PCL) and PCL/chitosan (CS) blend	439:506	Therefore, this study aims to fabricate nanofibers from a polycaprolactone (PCL) and PCL/chitosan (CS) blend by electrospinning, and to elucidate the effect of CS on the cellular response of mesothelial cells.					
36076916	2	41	theme	nanostructure	339:351	arg1	scaffold					353:360	a nanostructure scaffold	337:360	a nanostructure scaffold for tissue engineering applications	337:396	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a nanostructure scaffold for tissue engineering applications.					
36076916	1	42	theme	internal	233:240	arg1	organs					242:247	internal organs	233:247	internal organs	233:247	Mesothelial cells are specific epithelial cells lining the serosal cavity and internal organs.					
36076916	9	43	theme	PCL/CS	1479:1484	arg1	scaffold					1486:1493	The PCL/CS scaffold	1475:1493	The PCL/CS scaffold	1475:1493	The PCL/CS scaffold was therefore chosen for the in vivo studies, which involved transplanting a cell/scaffold construct containing allograft mesothelial cells for mesothelium reconstruction in rats.					
36076916	2	44	theme	engineering	373:383	arg1	applications					385:396	tissue engineering applications	366:396	tissue engineering applications	366:396	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a nanostructure scaffold for tissue engineering applications.					
36076916	7	45	theme	nanofiber	1087:1095	arg1	membranes					1097:1105	both nanofiber membranes	1082:1105	both nanofiber membranes	1082:1105	The mesothelial cells maintain high viability in both nanofiber membranes, but PCL/CS provides better maintenance of cobblestone-like mesothelial morphology.					
36076916	5	46	theme	due	849:851	arg1	scaffold					840:847	the scaffold	836:847	the scaffold due to interference of PCL crystallinity in the nanofibers	836:906	Blending CS with PCL influenced the mechanical properties of the scaffold due to interference of PCL crystallinity in the nanofibers.					
36076916	1	47	theme	specific	177:184	arg1	cells					197:201	specific epithelial cells	177:201	specific epithelial cells lining the serosal cavity and internal organs	177:247	Mesothelial cells are specific epithelial cells lining the serosal cavity and internal organs.					
36076916	1	47	theme	specific	177:184	arg1	cells					167:171	Mesothelial cells	155:171	Mesothelial cells	155:171	Mesothelial cells are specific epithelial cells lining the serosal cavity and internal organs.					
36076916	0	48	theme	Preferred	60:68	arg1	Scaffold					70:77	a Preferred Scaffold	58:77	a Preferred Scaffold for the Culture of Mesothelial Cells and the Repair of Damaged Mesothelium	58:152	Polycaprolactone/Chitosan Composite Nanofiber Membrane as a Preferred Scaffold for the Culture of Mesothelial Cells and the Repair of Damaged Mesothelium.					
36076916	8	49	dep	proteins	1402:1409	arg1	endorsing					1412:1420	endorsing	1412:1420	endorsing PCL/CS to better maintain the mesothelial phenotype	1412:1472	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	8	50	theme	gene	1196:1199	arg1	analysis					1212:1219	gene expression analysis	1196:1219	gene expression analysis	1196:1219	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	4	51	theme	nanofiber	655:663	arg1	scaffold					674:681	a PCL and PCL/CS nanofiber membrane scaffold	638:681	a PCL and PCL/CS nanofiber membrane scaffold	638:681	The results demonstrate that a PCL and PCL/CS nanofiber membrane scaffold could be prepared with a comparable fiber diameter (~300 nm) and porosity for cell culture.					
36076916	7	52	theme	high	1064:1067	arg1	viability					1069:1077	high viability	1064:1077	high viability	1064:1077	The mesothelial cells maintain high viability in both nanofiber membranes, but PCL/CS provides better maintenance of cobblestone-like mesothelial morphology.					
36076916	2	53	theme	mesothelial	316:326	arg1	cells					328:332	culture mesothelial cells	308:332	culture mesothelial cells	308:332	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a nanostructure scaffold for tissue engineering applications.					
36076916	6	54	theme	scaffold	944:951	arg1	hydrophilicity					953:966	scaffold hydrophilicity	944:966	scaffold hydrophilicity	944:966	However, CS substantially improves scaffold hydrophilicity and results in a ~6-times-higher cell attachment rate in PCL/CS.					
36076916	0	55	theme	Mesothelium	142:152	arg1	Culture					87:93	the Culture	83:93	the Culture of Mesothelial Cells	83:114	Polycaprolactone/Chitosan Composite Nanofiber Membrane as a Preferred Scaffold for the Culture of Mesothelial Cells and the Repair of Damaged Mesothelium.					
36076916	0	55	theme	Mesothelium	142:152	arg1	Repair					124:129	the Repair	120:129	the Repair of Damaged Mesothelium	120:152	Polycaprolactone/Chitosan Composite Nanofiber Membrane as a Preferred Scaffold for the Culture of Mesothelial Cells and the Repair of Damaged Mesothelium.					
36076916	8	56	theme	CS	1275:1276	arg1	incorporation					1258:1270	the incorporation	1254:1270	the incorporation of CS	1254:1276	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	8	57	theme	immunofluorescence	1225:1242	arg1	staining					1244:1251	immunofluorescence staining	1225:1251	immunofluorescence staining	1225:1251	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	11	58	theme	cell/scaffold	1893:1905	arg1	construct					1907:1915	the cell/scaffold construct	1889:1915	the cell/scaffold construct	1889:1915	In contrast, a mesothelium layer similar to native mesothelium tissue could be obtained by implanting the cell/scaffold construct, based on hematoxylin and eosin (H&E) and immunohistochemical staining.					
36076916	3	59	theme	cellular	569:576	arg1	response					578:585	the cellular response	565:585	the cellular response of mesothelial cells	565:606	Therefore, this study aims to fabricate nanofibers from a polycaprolactone (PCL) and PCL/chitosan (CS) blend by electrospinning, and to elucidate the effect of CS on the cellular response of mesothelial cells.					
36076916	9	60	theme	in	1524:1525	arg1	studies					1532:1538	the in vivo studies	1520:1538	the in vivo studies	1520:1538	The PCL/CS scaffold was therefore chosen for the in vivo studies, which involved transplanting a cell/scaffold construct containing allograft mesothelial cells for mesothelium reconstruction in rats.					
36076916	8	61	theme	mesothelial	1324:1334	arg1	genes					1343:1347	mesothelial marker genes	1324:1347	mesothelial marker genes	1324:1347	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	3	62	theme	cells	602:606	arg1	response					578:585	the cellular response	565:585	the cellular response of mesothelial cells	565:606	Therefore, this study aims to fabricate nanofibers from a polycaprolactone (PCL) and PCL/chitosan (CS) blend by electrospinning, and to elucidate the effect of CS on the cellular response of mesothelial cells.					
36076916	6	63	theme	attachment	1006:1015	arg1	rate					1017:1020	a ~6-times-higher cell attachment rate	983:1020	a ~6-times-higher cell attachment rate in PCL/CS	983:1030	However, CS substantially improves scaffold hydrophilicity and results in a ~6-times-higher cell attachment rate in PCL/CS.					
36076916	8	64	theme	genes	1343:1347	arg1	expression					1310:1319	the upregulated expression	1294:1319	the upregulated expression of mesothelial marker genes	1294:1347	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	8	64	theme	genes	1343:1347	arg1	production					1366:1375	the enhanced production	1353:1375	the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype	1353:1472	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	5	65	theme	PCL	872:874	arg1	crystallinity					876:888	PCL crystallinity	872:888	PCL crystallinity	872:888	Blending CS with PCL influenced the mechanical properties of the scaffold due to interference of PCL crystallinity in the nanofibers.					
36076916	6	66	theme	~6-times-higher	985:999	arg1	rate					1017:1020	a ~6-times-higher cell attachment rate	983:1020	a ~6-times-higher cell attachment rate in PCL/CS	983:1030	However, CS substantially improves scaffold hydrophilicity and results in a ~6-times-higher cell attachment rate in PCL/CS.					
36076916	11	67	theme	mesothelium	1838:1848	arg1	tissue					1850:1855	native mesothelium tissue	1831:1855	native mesothelium tissue	1831:1855	In contrast, a mesothelium layer similar to native mesothelium tissue could be obtained by implanting the cell/scaffold construct, based on hematoxylin and eosin (H&E) and immunohistochemical staining.					
36076916	0	68	theme	Cells	110:114	arg1	Culture					87:93	the Culture	83:93	the Culture of Mesothelial Cells	83:114	Polycaprolactone/Chitosan Composite Nanofiber Membrane as a Preferred Scaffold for the Culture of Mesothelial Cells and the Repair of Damaged Mesothelium.					
36076916	0	68	theme	Cells	110:114	arg1	Repair					124:129	the Repair	120:129	the Repair of Damaged Mesothelium	120:152	Polycaprolactone/Chitosan Composite Nanofiber Membrane as a Preferred Scaffold for the Culture of Mesothelial Cells and the Repair of Damaged Mesothelium.					
36076916	9	69	theme	cell/scaffold	1572:1584	arg1	construct					1586:1594	a cell/scaffold construct	1570:1594	a cell/scaffold construct containing allograft mesothelial cells for mesothelium reconstruction in rats	1570:1672	The PCL/CS scaffold was therefore chosen for the in vivo studies, which involved transplanting a cell/scaffold construct containing allograft mesothelial cells for mesothelium reconstruction in rats.					
36076916	2	70	theme	culture	308:314	arg1	cells					328:332	culture mesothelial cells	308:332	culture mesothelial cells	308:332	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a nanostructure scaffold for tissue engineering applications.					
36076916	6	71	from	rate	1017:1020	arg1	PCL/CS					1025:1030	PCL/CS	1025:1030	PCL/CS	1025:1030	However, CS substantially improves scaffold hydrophilicity and results in a ~6-times-higher cell attachment rate in PCL/CS.					
36076916	1	72	theme	serosal	214:220	arg1	cavity					222:227	the serosal cavity	210:227	the serosal cavity	210:227	Mesothelial cells are specific epithelial cells lining the serosal cavity and internal organs.					
36076916	8	73	theme	key	1380:1382	arg1	proteins					1402:1409	key mesothelial maker proteins	1380:1409	key mesothelial maker proteins	1380:1409	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	11	74	theme	immunohistochemical	1959:1977	arg1	staining					1979:1986	immunohistochemical staining	1959:1986	immunohistochemical staining	1959:1986	In contrast, a mesothelium layer similar to native mesothelium tissue could be obtained by implanting the cell/scaffold construct, based on hematoxylin and eosin (H&E) and immunohistochemical staining.					
36076916	9	75	theme	mesothelial	1617:1627	arg1	cells					1629:1633	allograft mesothelial cells	1607:1633	allograft mesothelial cells for mesothelium reconstruction in rats	1607:1672	The PCL/CS scaffold was therefore chosen for the in vivo studies, which involved transplanting a cell/scaffold construct containing allograft mesothelial cells for mesothelium reconstruction in rats.					
36076916	8	76	theme	maker	1396:1400	arg1	proteins					1402:1409	key mesothelial maker proteins	1380:1409	key mesothelial maker proteins	1380:1409	From gene expression analysis and immunofluorescence staining, the incorporation of CS also results in the upregulated expression of mesothelial marker genes and the enhanced production of key mesothelial maker proteins, endorsing PCL/CS to better maintain the mesothelial phenotype.					
36076916	10	77	theme	mesothelial	1693:1703	arg1	cells					1705:1709	mesothelial cells	1693:1709	mesothelial cells	1693:1709	In the absence of mesothelial cells, the mesothelium wound covered with PCL/CS showed an inflammatory response.					
36076916	7	78	theme	mesothelial	1167:1177	arg1	morphology					1179:1188	cobblestone-like mesothelial morphology	1150:1188	cobblestone-like mesothelial morphology	1150:1188	The mesothelial cells maintain high viability in both nanofiber membranes, but PCL/CS provides better maintenance of cobblestone-like mesothelial morphology.					
36076916	9	79	dep	in	1524:1525	arg1	vivo					1527:1530	vivo	1527:1530	vivo	1527:1530	The PCL/CS scaffold was therefore chosen for the in vivo studies, which involved transplanting a cell/scaffold construct containing allograft mesothelial cells for mesothelium reconstruction in rats.					
36076916	4	80	theme	PCL	640:642	arg1	scaffold					674:681	a PCL and PCL/CS nanofiber membrane scaffold	638:681	a PCL and PCL/CS nanofiber membrane scaffold	638:681	The results demonstrate that a PCL and PCL/CS nanofiber membrane scaffold could be prepared with a comparable fiber diameter (~300 nm) and porosity for cell culture.					
36076916	10	81	theme	mesothelium	1716:1726	arg1	wound					1728:1732	the mesothelium wound	1712:1732	the mesothelium wound covered with PCL/CS	1712:1752	In the absence of mesothelial cells, the mesothelium wound covered with PCL/CS showed an inflammatory response.					
36076916	7	82	theme	better	1128:1133	arg1	maintenance					1135:1145	better maintenance	1128:1145	better maintenance of cobblestone-like mesothelial morphology	1128:1188	The mesothelial cells maintain high viability in both nanofiber membranes, but PCL/CS provides better maintenance of cobblestone-like mesothelial morphology.					
36076916	3	83	dep	polycaprolactone	457:472	arg1	blend					502:506	blend	502:506	blend	502:506	Therefore, this study aims to fabricate nanofibers from a polycaprolactone (PCL) and PCL/chitosan (CS) blend by electrospinning, and to elucidate the effect of CS on the cellular response of mesothelial cells.					
36076916	4	84	theme	cell	761:764	arg1	culture					766:772	cell culture	761:772	cell culture	761:772	The results demonstrate that a PCL and PCL/CS nanofiber membrane scaffold could be prepared with a comparable fiber diameter (~300 nm) and porosity for cell culture.					
36076916	9	85	contain	containing	1596:1605	arg2	cells					1629:1633	allograft mesothelial cells	1607:1633	allograft mesothelial cells for mesothelium reconstruction in rats	1607:1672	The PCL/CS scaffold was therefore chosen for the in vivo studies, which involved transplanting a cell/scaffold construct containing allograft mesothelial cells for mesothelium reconstruction in rats.					
36076916	9	85	contain	containing	1596:1605	arg1	construct					1586:1594	a cell/scaffold construct	1570:1594	a cell/scaffold construct containing allograft mesothelial cells for mesothelium reconstruction in rats	1570:1672	The PCL/CS scaffold was therefore chosen for the in vivo studies, which involved transplanting a cell/scaffold construct containing allograft mesothelial cells for mesothelium reconstruction in rats.					
36076916	3	86	from	polycaprolactone	457:472	arg1	nanofibers					439:448	nanofibers	439:448	nanofibers from a polycaprolactone (PCL) and PCL/chitosan (CS) blend	439:506	Therefore, this study aims to fabricate nanofibers from a polycaprolactone (PCL) and PCL/chitosan (CS) blend by electrospinning, and to elucidate the effect of CS on the cellular response of mesothelial cells.					
35836350	0	0	theme	artificial	91:100	arg1	conduit					117:123	porous artificial nerve guidance conduit	84:123	porous artificial nerve guidance conduit	84:123	Preparation and mechanical optimization of a two-layer silk/magnesium wires braided porous artificial nerve guidance conduit.					
35836350	4	1	theme	optimal	694:700	arg1	combination					702:712	the optimal combination	690:712	the optimal combination of technical process parameters	690:744	Orthogonal experimental design was applied to rationally design the braided structural layer and obtain the optimal combination of technical process parameters.					
35836350	1	2	theme	guidance	251:258	arg1	conduits					260:267	nerve guidance conduits	245:267	nerve guidance conduits (NGCs)	245:274	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	1	2	theme	guidance	251:258	arg1	NGCs					270:273	NGCs	270:273	NGCs	270:273	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	0	3	theme	porous	84:89	arg1	conduit					117:123	porous artificial nerve guidance conduit	84:123	porous artificial nerve guidance conduit	84:123	Preparation and mechanical optimization of a two-layer silk/magnesium wires braided porous artificial nerve guidance conduit.					
35836350	1	4	theme	tricky	168:173	arg1	injures					143:149	Peripheral nerve injures	126:149	Peripheral nerve injures	126:149	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	1	4	theme	tricky	168:173	arg1	problem					175:181	a tricky problem	166:181	a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs)	166:274	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	1	5	theme	conduits	260:267	arg1	transplantation					226:240	the transplantation	222:240	the transplantation of nerve guidance conduits (NGCs)	222:274	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	0	6	theme	guidance	108:115	arg1	conduit					117:123	porous artificial nerve guidance conduit	84:123	porous artificial nerve guidance conduit	84:123	Preparation and mechanical optimization of a two-layer silk/magnesium wires braided porous artificial nerve guidance conduit.					
35836350	6	7	theme	good	927:930	arg1	biocompatibility					932:947	good biocompatibility	927:947	good biocompatibility	927:947	In vitro and in vivo study suggested that the textile-forming scaffold exhibited good biocompatibility and no toxicity.					
35836350	7	8	theme	uniform	1137:1143	arg1	dispersion					1145:1154	uniform dispersion	1137:1154	uniform dispersion	1137:1154	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	3	9	theme	fibroin/chitosan	530:545	arg1	SF/CS					548:552	silk fibroin/chitosan (SF/CS)	525:553	silk fibroin/chitosan (SF/CS) using freeze-drying treatment	525:583	The inner layer was made of degummed silk yarns/magnesium wires using braiding technology, and the outer layer was made from mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment.					
35836350	4	10	theme	experimental	597:608	arg1	design					610:615	Orthogonal experimental design	586:615	Orthogonal experimental design	586:615	Orthogonal experimental design was applied to rationally design the braided structural layer and obtain the optimal combination of technical process parameters.					
35836350	0	11	theme	nerve	102:106	arg1	conduit					117:123	porous artificial nerve guidance conduit	84:123	porous artificial nerve guidance conduit	84:123	Preparation and mechanical optimization of a two-layer silk/magnesium wires braided porous artificial nerve guidance conduit.					
35836350	3	12	theme	freeze-drying	561:573	arg1	treatment					575:583	freeze-drying treatment	561:583	freeze-drying treatment	561:583	The inner layer was made of degummed silk yarns/magnesium wires using braiding technology, and the outer layer was made from mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment.					
35836350	1	13	theme	Peripheral	126:135	arg1	injures					143:149	Peripheral nerve injures	126:149	Peripheral nerve injures	126:149	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	1	13	theme	Peripheral	126:135	arg1	problem					175:181	a tricky problem	166:181	a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs)	166:274	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	7	14	attach	released	1059:1066	arg1	wires					1092:1096	degraded magnesium wires	1073:1096	degraded magnesium wires	1073:1096	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	7	14	attach	released	1059:1066	arg2	ions					1054:1057	magnesium ions	1044:1057	magnesium ions released from degraded magnesium wires	1044:1096	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	4	15	theme	Orthogonal	586:595	arg1	design					610:615	Orthogonal experimental design	586:615	Orthogonal experimental design	586:615	Orthogonal experimental design was applied to rationally design the braided structural layer and obtain the optimal combination of technical process parameters.					
35836350	6	16	theme	textile-forming	892:906	arg1	scaffold					908:915	the textile-forming scaffold	888:915	the textile-forming scaffold	888:915	In vitro and in vivo study suggested that the textile-forming scaffold exhibited good biocompatibility and no toxicity.					
35836350	3	17	theme	silk	525:528	arg1	SF/CS					548:552	silk fibroin/chitosan (SF/CS)	525:553	silk fibroin/chitosan (SF/CS) using freeze-drying treatment	525:583	The inner layer was made of degummed silk yarns/magnesium wires using braiding technology, and the outer layer was made from mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment.					
35836350	4	18	theme	structural	662:671	arg1	layer					673:677	the braided structural layer	650:677	the braided structural layer	650:677	Orthogonal experimental design was applied to rationally design the braided structural layer and obtain the optimal combination of technical process parameters.					
35836350	3	19	theme	inner	386:390	arg1	layer					392:396	The inner layer	382:396	The inner layer	382:396	The inner layer was made of degummed silk yarns/magnesium wires using braiding technology, and the outer layer was made from mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment.					
35836350	7	20	theme	NGCs	1254:1257	arg1	development					1239:1249	the development	1235:1249	the development of NGCs	1235:1257	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	2	21	theme	composite	309:317	arg1	NGC					319:321	a two-layer composite NGC	297:321	a two-layer composite NGC with fair mechanical properties and good biocompatibility	297:379	This study presents a two-layer composite NGC with fair mechanical properties and good biocompatibility.					
35836350	3	22	theme	silk	419:422	arg1	wires					440:444	degummed silk yarns/magnesium wires	410:444	degummed silk yarns/magnesium wires	410:444	The inner layer was made of degummed silk yarns/magnesium wires using braiding technology, and the outer layer was made from mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment.					
35836350	5	23	theme	SF/CS	830:834	arg1	solution					836:843	SF/CS solution	830:843	SF/CS solution	830:843	Meanwhile, the SF/CS porous outer layer was optimized from three concentrations of SF/CS solution.					
35836350	4	24	theme	parameters	735:744	arg1	combination					702:712	the optimal combination	690:712	the optimal combination of technical process parameters	690:744	Orthogonal experimental design was applied to rationally design the braided structural layer and obtain the optimal combination of technical process parameters.					
35836350	7	25	theme	magnesium	1082:1090	arg1	wires					1092:1096	degraded magnesium wires	1073:1096	degraded magnesium wires	1073:1096	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	2	26	theme	two-layer	299:307	arg1	NGC					319:321	a two-layer composite NGC	297:321	a two-layer composite NGC with fair mechanical properties and good biocompatibility	297:379	This study presents a two-layer composite NGC with fair mechanical properties and good biocompatibility.					
35836350	3	27	theme	yarns/magnesium	424:438	arg1	wires					440:444	degummed silk yarns/magnesium wires	410:444	degummed silk yarns/magnesium wires	410:444	The inner layer was made of degummed silk yarns/magnesium wires using braiding technology, and the outer layer was made from mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment.					
35836350	5	28	theme	solution	836:843	arg1	concentrations					812:825	three concentrations	806:825	three concentrations of SF/CS solution	806:843	Meanwhile, the SF/CS porous outer layer was optimized from three concentrations of SF/CS solution.					
35836350	6	29	dep	In	846:847	arg1	vitro					849:853	vitro	849:853	vitro	849:853	In vitro and in vivo study suggested that the textile-forming scaffold exhibited good biocompatibility and no toxicity.					
35836350	3	30	theme	SF/CS	548:552	arg1	solution					513:520	mixed solution	507:520	mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment	507:583	The inner layer was made of degummed silk yarns/magnesium wires using braiding technology, and the outer layer was made from mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment.					
35836350	0	31	theme	mechanical	16:25	arg1	optimization					27:38	mechanical optimization	16:38	mechanical optimization of a two-layer silk/magnesium wires	16:74	Preparation and mechanical optimization of a two-layer silk/magnesium wires braided porous artificial nerve guidance conduit.					
35836350	6	32	dep	in	859:860	arg1	vivo					862:865	vivo	862:865	vivo	862:865	In vitro and in vivo study suggested that the textile-forming scaffold exhibited good biocompatibility and no toxicity.					
35836350	7	33	from	approach	1223:1230	arg1	development					1239:1249	the development	1235:1249	the development of NGCs	1235:1257	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	1	34	theme	feasible	200:207	arg1	treatment					209:217	a feasible treatment	198:217	a feasible treatment is the transplantation of nerve guidance conduits (NGCs)	198:274	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	2	35	theme	fair	328:331	arg1	properties					344:353	fair mechanical properties	328:353	fair mechanical properties	328:353	This study presents a two-layer composite NGC with fair mechanical properties and good biocompatibility.					
35836350	7	36	theme	degraded	1073:1080	arg1	wires					1092:1096	degraded magnesium wires	1073:1096	degraded magnesium wires	1073:1096	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	2	37	with	NGC	319:321	arg1	properties					344:353	fair mechanical properties	328:353	fair mechanical properties	328:353	This study presents a two-layer composite NGC with fair mechanical properties and good biocompatibility.					
35836350	2	37	with	NGC	319:321	arg1	biocompatibility					364:379	good biocompatibility	359:379	good biocompatibility	359:379	This study presents a two-layer composite NGC with fair mechanical properties and good biocompatibility.					
35836350	3	38	theme	braiding	452:459	arg1	technology					461:470	braiding technology	452:470	braiding technology	452:470	The inner layer was made of degummed silk yarns/magnesium wires using braiding technology, and the outer layer was made from mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment.					
35836350	3	39	theme	degummed	410:417	arg1	wires					440:444	degummed silk yarns/magnesium wires	410:444	degummed silk yarns/magnesium wires	410:444	The inner layer was made of degummed silk yarns/magnesium wires using braiding technology, and the outer layer was made from mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment.					
35836350	7	40	theme	Schwann	1187:1193	arg1	cells					1195:1199	Schwann cells	1187:1199	Schwann cells	1187:1199	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	4	41	theme	technical	717:725	arg1	parameters					735:744	technical process parameters	717:744	technical process parameters	717:744	Orthogonal experimental design was applied to rationally design the braided structural layer and obtain the optimal combination of technical process parameters.					
35836350	7	42	theme	cells	1195:1199	arg1	adhesion					1175:1182	adhesion	1175:1182	adhesion	1175:1182	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	7	42	theme	cells	1195:1199	arg1	proliferation					1157:1169	proliferation	1157:1169	proliferation	1157:1169	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	5	43	theme	SF/CS	762:766	arg1	Meanwhile					747:755	Meanwhile	747:755	Meanwhile	747:755	Meanwhile, the SF/CS porous outer layer was optimized from three concentrations of SF/CS solution.					
35836350	5	43	theme	SF/CS	762:766	arg1	layer					781:785	the SF/CS porous outer layer	758:785	the SF/CS porous outer layer	758:785	Meanwhile, the SF/CS porous outer layer was optimized from three concentrations of SF/CS solution.					
35836350	0	44	theme	silk/magnesium	55:68	arg1	wires					70:74	a two-layer silk/magnesium wires	43:74	a two-layer silk/magnesium wires	43:74	Preparation and mechanical optimization of a two-layer silk/magnesium wires braided porous artificial nerve guidance conduit.					
35836350	4	45	theme	braided	654:660	arg1	layer					673:677	the braided structural layer	650:677	the braided structural layer	650:677	Orthogonal experimental design was applied to rationally design the braided structural layer and obtain the optimal combination of technical process parameters.					
35836350	5	46	theme	outer	775:779	arg1	Meanwhile					747:755	Meanwhile	747:755	Meanwhile	747:755	Meanwhile, the SF/CS porous outer layer was optimized from three concentrations of SF/CS solution.					
35836350	5	46	theme	outer	775:779	arg1	layer					781:785	the SF/CS porous outer layer	758:785	the SF/CS porous outer layer	758:785	Meanwhile, the SF/CS porous outer layer was optimized from three concentrations of SF/CS solution.					
35836350	7	47	theme	magnesium	1044:1052	arg1	ions					1054:1057	magnesium ions	1044:1057	magnesium ions released from degraded magnesium wires	1044:1096	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	2	48	theme	mechanical	333:342	arg1	properties					344:353	fair mechanical properties	328:353	fair mechanical properties	328:353	This study presents a two-layer composite NGC with fair mechanical properties and good biocompatibility.					
35836350	0	49	theme	two-layer	45:53	arg1	wires					70:74	a two-layer silk/magnesium wires	43:74	a two-layer silk/magnesium wires	43:74	Preparation and mechanical optimization of a two-layer silk/magnesium wires braided porous artificial nerve guidance conduit.					
35836350	6	50	theme	in	859:860	arg1	study					867:871	In vitro and in vivo study	846:871	In vitro and in vivo study	846:871	In vitro and in vivo study suggested that the textile-forming scaffold exhibited good biocompatibility and no toxicity.					
35836350	1	51	theme	nerve	137:141	arg1	injures					143:149	Peripheral nerve injures	126:149	Peripheral nerve injures	126:149	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	1	51	theme	nerve	137:141	arg1	problem					175:181	a tricky problem	166:181	a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs)	166:274	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	4	52	theme	process	727:733	arg1	parameters					735:744	technical process parameters	717:744	technical process parameters	717:744	Orthogonal experimental design was applied to rationally design the braided structural layer and obtain the optimal combination of technical process parameters.					
35836350	1	53	dep	treatment	209:217	arg1	transplantation					226:240	the transplantation	222:240	the transplantation of nerve guidance conduits (NGCs)	222:274	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	7	54	theme	conduits	1011:1018	arg1	skeleton					999:1006	the skeleton	995:1006	the skeleton of conduits	995:1018	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	3	55	theme	outer	481:485	arg1	layer					487:491	the outer layer	477:491	the outer layer	477:491	The inner layer was made of degummed silk yarns/magnesium wires using braiding technology, and the outer layer was made from mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment.					
35836350	7	56	theme	sustainable	1113:1123	arg1	release					1125:1131	sustainable release	1113:1131	sustainable release	1113:1131	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	5	57	theme	porous	768:773	arg1	Meanwhile					747:755	Meanwhile	747:755	Meanwhile	747:755	Meanwhile, the SF/CS porous outer layer was optimized from three concentrations of SF/CS solution.					
35836350	5	57	theme	porous	768:773	arg1	layer					781:785	the SF/CS porous outer layer	758:785	the SF/CS porous outer layer	758:785	Meanwhile, the SF/CS porous outer layer was optimized from three concentrations of SF/CS solution.					
35836350	6	58	theme	In	846:847	arg1	study					867:871	In vitro and in vivo study	846:871	In vitro and in vivo study	846:871	In vitro and in vivo study suggested that the textile-forming scaffold exhibited good biocompatibility and no toxicity.					
35836350	3	59	theme	mixed	507:511	arg1	solution					513:520	mixed solution	507:520	mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment	507:583	The inner layer was made of degummed silk yarns/magnesium wires using braiding technology, and the outer layer was made from mixed solution of silk fibroin/chitosan (SF/CS) using freeze-drying treatment.					
35836350	0	60	theme	wires	70:74	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and mechanical optimization of a two-layer silk/magnesium wires braided porous artificial nerve guidance conduit.					
35836350	0	60	theme	wires	70:74	arg1	optimization					27:38	mechanical optimization	16:38	mechanical optimization of a two-layer silk/magnesium wires	16:74	Preparation and mechanical optimization of a two-layer silk/magnesium wires braided porous artificial nerve guidance conduit.					
35836350	6	61	theme	no	953:954	arg1	toxicity					956:963	no toxicity	953:963	no toxicity	953:963	In vitro and in vivo study suggested that the textile-forming scaffold exhibited good biocompatibility and no toxicity.					
35836350	1	62	from	problem	175:181	arg1	surgery					186:192	surgery	186:192	surgery	186:192	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	1	62	from	problem	175:181	arg1	treatment					209:217	a feasible treatment	198:217	a feasible treatment is the transplantation of nerve guidance conduits (NGCs)	198:274	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	2	63	theme	good	359:362	arg1	biocompatibility					364:379	good biocompatibility	359:379	good biocompatibility	359:379	This study presents a two-layer composite NGC with fair mechanical properties and good biocompatibility.					
35836350	7	64	theme	potential	1213:1221	arg1	approach					1223:1230	potential approach	1213:1230	potential approach in the development of NGCs	1213:1257	During 4 weeks' degradation, the skeleton of conduits retained its shape, and magnesium ions released from degraded magnesium wires contributed to sustainable release and uniform dispersion, proliferation and adhesion of Schwann cells, indicating potential approach in the development of NGCs.					
35836350	1	65	theme	nerve	245:249	arg1	conduits					260:267	nerve guidance conduits	245:267	nerve guidance conduits (NGCs)	245:274	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
35836350	1	65	theme	nerve	245:249	arg1	NGCs					270:273	NGCs	270:273	NGCs	270:273	Peripheral nerve injures have long been a tricky problem in surgery and a feasible treatment is the transplantation of nerve guidance conduits (NGCs).					
36075479	0	0	theme	coal	73:76	arg1	pollution					83:91	coal dust pollution	73:91	coal dust pollution	73:91	Carboxymethyl cellulose sodium gel: A modified material used to suppress coal dust pollution.					
36075479	1	1	theme	coal	142:145	arg1	dust					147:150	coal dust	142:150	coal dust	142:150	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	4	2	theme	samples	883:889	arg1	retention					865:873	the water retention	855:873	the water retention of coal samples sprayed with just water	855:913	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
36075479	5	3	theme	water	1015:1019	arg1	times					1001:1005	6 times	999:1005	6 times that of water	999:1019	Additionally, the ability of the dust suppression agent to resist wind erosion was 6 times that of water.					
36075479	5	3	theme	water	1015:1019	arg1	ability					934:940	the ability	930:940	the ability of the dust suppression agent to resist wind erosion	930:993	Additionally, the ability of the dust suppression agent to resist wind erosion was 6 times that of water.					
36075479	5	4	theme	wind	982:985	arg1	erosion					987:993	wind erosion	982:993	wind erosion	982:993	Additionally, the ability of the dust suppression agent to resist wind erosion was 6 times that of water.					
36075479	1	5	theme	high	213:216	arg1	efficiency					218:227	high efficiency	213:227	high efficiency	213:227	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	3	6	theme	CMC-Na	523:528	arg1	copolymer					510:518	the graft copolymer	500:518	the graft copolymer of CMC-Na and AM (CMC-Na-co-AM)	500:550	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	1	7	theme	efficiency	218:227	arg1	type					159:162	a new type	153:162	a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity	153:254	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	0	8	theme	dust	78:81	arg1	pollution					83:91	coal dust pollution	73:91	coal dust pollution	73:91	Carboxymethyl cellulose sodium gel: A modified material used to suppress coal dust pollution.					
36075479	4	9	theme	water	804:808	arg1	%					795:795	>50%	792:795	>50% of the water	792:808	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
36075479	4	9	theme	water	804:808	arg1	water					804:808	the water	800:808	the water	800:808	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
36075479	4	10	from	60 °C	713:717	arg1	14 h					695:698	14 h	695:698	14 h of drying at 60 °C	695:717	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
36075479	6	11	theme	coal	1208:1211	arg1	production					1213:1222	coal production	1208:1222	coal production	1208:1222	The CMC-Na-co-AM dust suppression agent showed that it could effectively inhibit the spread of coal dust under strong winds, offering a solution to the problem of coal dust pollution in coal production and storage.					
36075479	4	12	theme	coal	720:723	arg1	samples					725:731	coal samples	720:731	coal samples that were sprayed with the dust suppression agent	720:781	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
36075479	2	13	theme	molecular	354:362	arg1	simulations					373:383	molecular dynamics simulations	354:383	molecular dynamics simulations	354:383	Through molecular dynamics simulations and experiments, the surfactant composition and concentration were optimized.					
36075479	4	14	theme	water	859:863	arg1	retention					865:873	the water retention	855:873	the water retention of coal samples sprayed with just water	855:913	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
36075479	6	15	theme	CMC-Na-co-AM	1026:1037	arg1	agent					1056:1060	The CMC-Na-co-AM dust suppression agent	1022:1060	The CMC-Na-co-AM dust suppression agent	1022:1060	The CMC-Na-co-AM dust suppression agent showed that it could effectively inhibit the spread of coal dust under strong winds, offering a solution to the problem of coal dust pollution in coal production and storage.					
36075479	1	16	with	simplicity	245:254	arg1	range					206:210	a wide application range	187:210	a wide application range	187:210	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	2	17	theme	surfactant	406:415	arg1	composition					417:427	the surfactant composition	402:427	the surfactant composition	402:427	Through molecular dynamics simulations and experiments, the surfactant composition and concentration were optimized.					
36075479	0	18	theme	cellulose	14:22	arg1	gel					31:33	Carboxymethyl cellulose sodium gel	0:33	Carboxymethyl cellulose sodium gel: A modified material used to suppress coal dust pollution.	0:92	Carboxymethyl cellulose sodium gel: A modified material used to suppress coal dust pollution.					
36075479	3	19	theme	coal	678:681	arg1	dust					683:686	coal dust	678:686	coal dust	678:686	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	6	20	theme	strong	1133:1138	arg1	winds					1140:1144	strong winds	1133:1144	strong winds	1133:1144	The CMC-Na-co-AM dust suppression agent showed that it could effectively inhibit the spread of coal dust under strong winds, offering a solution to the problem of coal dust pollution in coal production and storage.					
36075479	6	21	theme	suppression	1044:1054	arg1	agent					1056:1060	The CMC-Na-co-AM dust suppression agent	1022:1060	The CMC-Na-co-AM dust suppression agent	1022:1060	The CMC-Na-co-AM dust suppression agent showed that it could effectively inhibit the spread of coal dust under strong winds, offering a solution to the problem of coal dust pollution in coal production and storage.					
36075479	1	22	theme	new	155:157	arg1	type					159:162	a new type	153:162	a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity	153:254	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	0	23	theme	Carboxymethyl	0:12	arg1	gel					31:33	Carboxymethyl cellulose sodium gel	0:33	Carboxymethyl cellulose sodium gel: A modified material used to suppress coal dust pollution.	0:92	Carboxymethyl cellulose sodium gel: A modified material used to suppress coal dust pollution.					
36075479	6	24	theme	dust	1039:1042	arg1	agent					1056:1060	The CMC-Na-co-AM dust suppression agent	1022:1060	The CMC-Na-co-AM dust suppression agent	1022:1060	The CMC-Na-co-AM dust suppression agent showed that it could effectively inhibit the spread of coal dust under strong winds, offering a solution to the problem of coal dust pollution in coal production and storage.					
36075479	1	25	theme	production	234:243	arg1	simplicity					245:254	production simplicity	234:254	production simplicity	234:254	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	1	26	with	inhibitor	172:180	arg1	range					206:210	a wide application range	187:210	a wide application range	187:210	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	4	27	theme	dust	760:763	arg1	agent					777:781	the dust suppression agent	756:781	the dust suppression agent	756:781	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
36075479	3	28	theme	AM	534:535	arg1	copolymer					510:518	the graft copolymer	500:518	the graft copolymer of CMC-Na and AM (CMC-Na-co-AM)	500:550	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	0	29	theme	sodium	24:29	arg1	gel					31:33	Carboxymethyl cellulose sodium gel	0:33	Carboxymethyl cellulose sodium gel: A modified material used to suppress coal dust pollution.	0:92	Carboxymethyl cellulose sodium gel: A modified material used to suppress coal dust pollution.					
36075479	1	30	theme	simplicity	245:254	arg1	type					159:162	a new type	153:162	a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity	153:254	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	3	31	theme	fiber	607:611	arg1	structure					621:629	a unique fiber network structure	598:629	a unique fiber network structure	598:629	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	1	32	theme	dust	167:170	arg1	inhibitor					172:180	dust inhibitor	167:180	dust inhibitor with a wide application range	167:210	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	6	33	theme	pollution	1195:1203	arg1	problem					1174:1180	the problem	1170:1180	the problem of coal dust pollution in coal production and storage	1170:1234	The CMC-Na-co-AM dust suppression agent showed that it could effectively inhibit the spread of coal dust under strong winds, offering a solution to the problem of coal dust pollution in coal production and storage.					
36075479	3	34	theme	network	613:619	arg1	structure					621:629	a unique fiber network structure	598:629	a unique fiber network structure	598:629	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	6	35	theme	dust	1122:1125	arg1	spread					1107:1112	the spread	1103:1112	the spread of coal dust	1103:1125	The CMC-Na-co-AM dust suppression agent showed that it could effectively inhibit the spread of coal dust under strong winds, offering a solution to the problem of coal dust pollution in coal production and storage.					
36075479	1	36	theme	inhibitor	172:180	arg1	type					159:162	a new type	153:162	a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity	153:254	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	3	37	dep	had	552:554	arg1	increased					646:654	increased	646:654	increased its contact area with coal dust	646:686	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	6	38	from	problem	1174:1180	arg1	storage					1228:1234	storage	1228:1234	storage	1228:1234	The CMC-Na-co-AM dust suppression agent showed that it could effectively inhibit the spread of coal dust under strong winds, offering a solution to the problem of coal dust pollution in coal production and storage.					
36075479	6	38	from	problem	1174:1180	arg1	production					1213:1222	coal production	1208:1222	coal production	1208:1222	The CMC-Na-co-AM dust suppression agent showed that it could effectively inhibit the spread of coal dust under strong winds, offering a solution to the problem of coal dust pollution in coal production and storage.					
36075479	3	39	contain	had	552:554	arg1	copolymer					510:518	the graft copolymer	500:518	the graft copolymer of CMC-Na and AM (CMC-Na-co-AM)	500:550	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	3	39	contain	had	552:554	arg2	pores					561:565	more pores	556:565	more pores	556:565	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	6	40	theme	coal	1117:1120	arg1	dust					1122:1125	coal dust	1117:1125	coal dust	1117:1125	The CMC-Na-co-AM dust suppression agent showed that it could effectively inhibit the spread of coal dust under strong winds, offering a solution to the problem of coal dust pollution in coal production and storage.					
36075479	1	41	with	efficiency	218:227	arg1	range					206:210	a wide application range	187:210	a wide application range	187:210	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	2	42	theme	dynamics	364:371	arg1	simulations					373:383	molecular dynamics simulations	354:383	molecular dynamics simulations	354:383	Through molecular dynamics simulations and experiments, the surfactant composition and concentration were optimized.					
36075479	5	43	theme	agent	966:970	arg1	times					1001:1005	6 times	999:1005	6 times that of water	999:1019	Additionally, the ability of the dust suppression agent to resist wind erosion was 6 times that of water.					
36075479	5	43	theme	agent	966:970	arg1	ability					934:940	the ability	930:940	the ability of the dust suppression agent to resist wind erosion	930:993	Additionally, the ability of the dust suppression agent to resist wind erosion was 6 times that of water.					
36075479	5	44	dep	times	1001:1005	arg1	that					1007:1010	that	1007:1010	that	1007:1010	Additionally, the ability of the dust suppression agent to resist wind erosion was 6 times that of water.					
36075479	6	45	theme	coal	1185:1188	arg1	pollution					1195:1203	coal dust pollution	1185:1203	coal dust pollution	1185:1203	The CMC-Na-co-AM dust suppression agent showed that it could effectively inhibit the spread of coal dust under strong winds, offering a solution to the problem of coal dust pollution in coal production and storage.					
36075479	3	46	theme	unique	600:605	arg1	structure					621:629	a unique fiber network structure	598:629	a unique fiber network structure	598:629	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	4	47	theme	suppression	765:775	arg1	agent					777:781	the dust suppression agent	756:781	the dust suppression agent	756:781	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
36075479	3	48	theme	experimental	467:478	arg1	results					480:486	The experimental results	463:486	The experimental results	463:486	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	0	49	theme	modified	38:45	arg1	material					47:54	A modified material	36:54	Carboxymethyl cellulose sodium gel: A modified material used to suppress coal dust pollution.	0:92	Carboxymethyl cellulose sodium gel: A modified material used to suppress coal dust pollution.					
36075479	4	50	theme	9	834:834	arg1	times					836:840	times	836:840	times	836:840	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
36075479	4	50	theme	9	834:834	arg1	spray					817:821	the spray	813:821	the spray	813:821	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
36075479	3	51	theme	more	556:559	arg1	pores					561:565	more pores	556:565	more pores	556:565	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	1	52	theme	sodium	285:290	arg1	CMC-Na					316:321	CMC-Na	316:321	CMC-Na	316:321	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	1	52	theme	sodium	285:290	arg1	carboxymethylcellulose					292:313	sodium carboxymethylcellulose	285:313	sodium carboxymethylcellulose (CMC-Na)	285:322	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	0	53	dep	gel	31:33	arg1	material					47:54	A modified material	36:54	Carboxymethyl cellulose sodium gel: A modified material used to suppress coal dust pollution.	0:92	Carboxymethyl cellulose sodium gel: A modified material used to suppress coal dust pollution.					
36075479	1	54	theme	environmental	108:120	arg1	pollution					122:130	the environmental pollution	104:130	the environmental pollution caused by coal dust	104:150	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	4	55	theme	drying	703:708	arg1	14 h					695:698	14 h	695:698	14 h of drying at 60 °C	695:717	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
36075479	6	56	theme	dust	1190:1193	arg1	pollution					1195:1203	coal dust pollution	1185:1203	coal dust pollution	1185:1203	The CMC-Na-co-AM dust suppression agent showed that it could effectively inhibit the spread of coal dust under strong winds, offering a solution to the problem of coal dust pollution in coal production and storage.					
36075479	5	57	theme	dust	949:952	arg1	agent					966:970	the dust suppression agent	945:970	the dust suppression agent	945:970	Additionally, the ability of the dust suppression agent to resist wind erosion was 6 times that of water.					
36075479	1	58	theme	wide	189:192	arg1	range					206:210	a wide application range	187:210	a wide application range	187:210	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	4	59	theme	coal	878:881	arg1	samples					883:889	coal samples	878:889	coal samples sprayed with just water	878:913	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
36075479	5	60	theme	suppression	954:964	arg1	agent					966:970	the dust suppression agent	945:970	the dust suppression agent	945:970	Additionally, the ability of the dust suppression agent to resist wind erosion was 6 times that of water.					
36075479	1	61	theme	application	194:204	arg1	range					206:210	a wide application range	187:210	a wide application range	187:210	To reduce the environmental pollution caused by coal dust, a new type of dust inhibitor with a wide application range, high efficiency, and production simplicity was synthesized by modifying sodium carboxymethylcellulose (CMC-Na) with acrylamide (AM).					
36075479	3	62	theme	contact	660:666	arg1	area					668:671	its contact area	656:671	its contact area	656:671	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	3	63	theme	microscopic	574:584	arg1	surface					586:592	the microscopic surface	570:592	the microscopic surface	570:592	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	3	64	theme	graft	504:508	arg1	copolymer					510:518	the graft copolymer	500:518	the graft copolymer of CMC-Na and AM (CMC-Na-co-AM)	500:550	The experimental results showed that the graft copolymer of CMC-Na and AM (CMC-Na-co-AM) had more pores on the microscopic surface and a unique fiber network structure, which greatly increased its contact area with coal dust.					
36075479	4	65	dep	times	836:840	arg1	greater					842:848	greater	842:848	greater	842:848	After 14 h of drying at 60 °C, coal samples that were sprayed with the dust suppression agent retained >50% of the water in the spray, which was 9 times greater than the water retention of coal samples sprayed with just water.					
35755600	4	0	theme	spectrometry	852:863	arg1	platform					865:872	a high-throughput liquid chromatography-tandem mass spectrometry platform	800:872	a high-throughput liquid chromatography-tandem mass spectrometry platform	800:872	We used a high-throughput liquid chromatography-tandem mass spectrometry platform to assess serum levels of N-glycans up to 3 days after injury.					
35755600	15	1	theme	larger	2522:2527	arg1	studies					2541:2547	Further larger multicenter studies	2514:2547	Further larger multicenter studies	2514:2547	Further larger multicenter studies will be required to validate our findings and to determine their pathobiological value and potential applications in practice.					
35755600	6	2	theme	principal	1080:1088	arg1	analysis					1100:1107	principal component analysis	1080:1107	principal component analysis	1080:1107	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	14	3	theme	patients	2335:2342	arg1	nature					2280:2285	the exploratory nature	2264:2285	the exploratory nature of the study	2264:2298	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	14	3	theme	patients	2335:2342	arg1	number					2325:2330	the relatively small number	2304:2330	the relatively small number of patients	2304:2342	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	1	4	theme	brain	265:269	arg1	disorders					271:279	numerous brain disorders	256:279	numerous brain disorders	256:279	Background Glycans play essential functional roles in the nervous system and their pathobiological relevance has become increasingly recognized in numerous brain disorders, but not fully explored in traumatic brain injury (TBI).					
35755600	2	5	with	patients	387:394	arg1	score					450:454	Glasgow Coma Scale [GCS] score ≤12	425:458	Glasgow Coma Scale [GCS] score ≤12	425:458	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	2	5	with	patients	387:394	arg1	TBI					420:422	moderate to severe TBI	401:422	moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12)	401:459	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	14	6	theme	number	2325:2330	arg1	spite					2255:2259	spite	2255:2259	spite of the exploratory nature of the study and the relatively small number of patients	2255:2342	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	4	7	theme	high-throughput	802:816	arg1	platform					865:872	a high-throughput liquid chromatography-tandem mass spectrometry platform	800:872	a high-throughput liquid chromatography-tandem mass spectrometry platform	800:872	We used a high-throughput liquid chromatography-tandem mass spectrometry platform to assess serum levels of N-glycans up to 3 days after injury.					
35755600	16	8	dep	funded	2698:2703	arg1	supported					2791:2799	supported	2791:2799	supported by a NIH grant (1R01GM112490-08)	2791:2832	Funding This work was funded by the Italian Ministry of Health (grant number GR-2013-02354960), and also partially supported by a NIH grant (1R01GM112490-08).					
35755600	14	9	theme	patient	2486:2492	arg1	phenotyping					2494:2504	patient phenotyping	2486:2504	patient phenotyping in TBI	2486:2511	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	1	10	theme	nervous	167:173	arg1	system					175:180	the nervous system	163:180	the nervous system	163:180	Background Glycans play essential functional roles in the nervous system and their pathobiological relevance has become increasingly recognized in numerous brain disorders, but not fully explored in traumatic brain injury (TBI).					
35755600	4	11	theme	chromatography-tandem	825:845	arg1	spectrometry					852:863	liquid chromatography-tandem mass spectrometry	818:863	a high-throughput liquid chromatography-tandem mass spectrometry platform	800:872	We used a high-throughput liquid chromatography-tandem mass spectrometry platform to assess serum levels of N-glycans up to 3 days after injury.					
35755600	14	12	from	discovery	2472:2480	arg1	TBI					2509:2511	TBI	2509:2511	TBI	2509:2511	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	6	13	theme	class	1254:1258	arg1	distinction					1260:1270	class distinction	1254:1270	class distinction	1254:1270	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	3	14	with	patients	644:651	arg1	TBI					658:660	TBI	658:660	TBI	658:660	Methods This prospective single-center observational study included 51 adult patients with TBI (GCS ≤12) admitted to the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019.					
35755600	12	15	theme	unfavorable	2077:2087	arg1	outcome					2089:2095	unfavorable outcome	2077:2095	unfavorable outcome	2077:2095	Serum levels of HexNAc7Hex7DeoxyHex1NeuAc2 and HexNAc8Hex6DeoxyHex0NeuAc0 were persistently increased in patients with favorable outcome, but undetectable in those with unfavorable outcome.					
35755600	6	16	theme	discriminant	1146:1157	arg1	analysis					1159:1166	orthogonal partial least squares discriminant analysis	1113:1166	orthogonal partial least squares discriminant analysis	1113:1166	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	9	17	theme	mannose	1513:1519	arg1	Levels					1498:1503	Levels	1498:1503	Levels of high mannose, hybrid and sialylated structures	1498:1553	Levels of high mannose, hybrid and sialylated structures were temporally altered (p<0·05).					
35755600	1	18	theme	Background	109:118	arg1	Glycans					120:126	Background Glycans	109:126	Background Glycans	109:126	Background Glycans play essential functional roles in the nervous system and their pathobiological relevance has become increasingly recognized in numerous brain disorders, but not fully explored in traumatic brain injury (TBI).					
35755600	2	19	theme	clinical	526:533	arg1	features					535:542	clinical features	526:542	clinical features	526:542	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	14	20	theme	nature	2280:2285	arg1	spite					2255:2259	spite	2255:2259	spite of the exploratory nature of the study and the relatively small number of patients	2255:2342	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	4	21	dep	days	918:921	arg1	injury					929:934	injury	929:934	injury	929:934	We used a high-throughput liquid chromatography-tandem mass spectrometry platform to assess serum levels of N-glycans up to 3 days after injury.					
35755600	3	22	theme	observational	606:618	arg1	study					620:624	This prospective single-center observational study	575:624	This prospective single-center observational study	575:624	Methods This prospective single-center observational study included 51 adult patients with TBI (GCS ≤12) admitted to the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019.					
35755600	9	23	theme	hybrid	1522:1527	arg1	Levels					1498:1503	Levels	1498:1503	Levels of high mannose, hybrid and sialylated structures	1498:1553	Levels of high mannose, hybrid and sialylated structures were temporally altered (p<0·05).					
35755600	16	24	theme	number	2746:2751	arg1	GR-2013-02354960					2753:2768	grant number GR-2013-02354960	2740:2768	grant number GR-2013-02354960	2740:2768	Funding This work was funded by the Italian Ministry of Health (grant number GR-2013-02354960), and also partially supported by a NIH grant (1R01GM112490-08).					
35755600	16	24	theme	number	2746:2751	arg1	Health					2732:2737	Health	2732:2737	Health (grant number GR-2013-02354960)	2732:2769	Funding This work was funded by the Italian Ministry of Health (grant number GR-2013-02354960), and also partially supported by a NIH grant (1R01GM112490-08).					
35755600	4	25	theme	serum	884:888	arg1	levels					890:895	serum levels	884:895	serum levels of N-glycans	884:908	We used a high-throughput liquid chromatography-tandem mass spectrometry platform to assess serum levels of N-glycans up to 3 days after injury.					
35755600	6	26	theme	influential	1223:1233	arg1	structures					1235:1244	highly influential structures	1216:1244	highly influential structures driving class distinction	1216:1270	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	15	27	from	value	2630:2634	arg1	practice					2666:2673	practice	2666:2673	practice	2666:2673	Further larger multicenter studies will be required to validate our findings and to determine their pathobiological value and potential applications in practice.					
35755600	3	28	theme	prospective	580:590	arg1	study					620:624	This prospective single-center observational study	575:624	This prospective single-center observational study	575:624	Methods This prospective single-center observational study included 51 adult patients with TBI (GCS ≤12) admitted to the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019.					
35755600	9	29	theme	sialylated	1533:1542	arg1	structures					1544:1553	sialylated structures	1533:1553	sialylated structures	1533:1553	Levels of high mannose, hybrid and sialylated structures were temporally altered (p<0·05).					
35755600	14	30	theme	approaches	2447:2456	arg1	utility					2426:2432	the utility	2422:2432	the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI	2422:2511	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	5	31	theme	post-injury	1020:1030	arg1	months					1013:1018	12 months post-injury	1010:1030	12 months post-injury	1010:1030	Outcome was assessed using the Glasgow Outcome Scale-Extended (GOS-E) at 12 months post-injury.					
35755600	3	32	theme	adult	638:642	arg1	patients					644:651	51 adult patients	635:651	51 adult patients with TBI (GCS ≤12) admitted to the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019	635:789	Methods This prospective single-center observational study included 51 adult patients with TBI (GCS ≤12) admitted to the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019.					
35755600	3	32	theme	adult	638:642	arg1	GCS					663:665	GCS ≤12	663:669	GCS ≤12	663:669	Methods This prospective single-center observational study included 51 adult patients with TBI (GCS ≤12) admitted to the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019.					
35755600	2	33	theme	glyco-biomarker	477:491	arg1	signatures					493:502	glyco-biomarker signatures	477:502	glyco-biomarker signatures	477:502	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	0	34	theme	traumatic	58:66	arg1	injury					74:79	moderate to severe traumatic brain injury	39:79	moderate to severe traumatic brain injury	39:79	Exploring serum glycome patterns after moderate to severe traumatic brain injury: A prospective pilot study.					
35755600	1	35	theme	functional	143:152	arg1	roles					154:158	essential functional roles	133:158	essential functional roles	133:158	Background Glycans play essential functional roles in the nervous system and their pathobiological relevance has become increasingly recognized in numerous brain disorders, but not fully explored in traumatic brain injury (TBI).					
35755600	11	36	theme	[95	1849:1851	arg1	CI					1853:1854	=0·75 [95%CI 0·59-0·90	1843:1864	=0·75 [95%CI 0·59-0·90	1843:1864	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	3	37	dep	Methods	567:573	arg1	included					626:633	included	626:633	included 51 adult patients with TBI (GCS ≤12) admitted to the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019	626:789	Methods This prospective single-center observational study included 51 adult patients with TBI (GCS ≤12) admitted to the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019.					
35755600	9	38	dep	altered	1571:1577	arg1	p<0·05					1580:1585	p<0·05	1580:1585	p<0·05	1580:1585	Levels of high mannose, hybrid and sialylated structures were temporally altered (p<0·05).					
35755600	15	39	theme	multicenter	2529:2539	arg1	studies					2541:2547	Further larger multicenter studies	2514:2547	Further larger multicenter studies	2514:2547	Further larger multicenter studies will be required to validate our findings and to determine their pathobiological value and potential applications in practice.					
35755600	16	40	theme	Italian	2712:2718	arg1	Ministry					2720:2727	the Italian Ministry	2708:2727	the Italian Ministry of Health (grant number GR-2013-02354960)	2708:2769	Funding This work was funded by the Italian Ministry of Health (grant number GR-2013-02354960), and also partially supported by a NIH grant (1R01GM112490-08).					
35755600	0	41	theme	pilot	96:100	arg1	study					102:106	A prospective pilot study	82:106	A prospective pilot study	82:106	Exploring serum glycome patterns after moderate to severe traumatic brain injury: A prospective pilot study.					
35755600	15	42	theme	Further	2514:2520	arg1	studies					2541:2547	Further larger multicenter studies	2514:2547	Further larger multicenter studies	2514:2547	Further larger multicenter studies will be required to validate our findings and to determine their pathobiological value and potential applications in practice.					
35755600	12	43	theme	HexNAc8Hex6DeoxyHex0NeuAc0	1955:1980	arg1	levels					1914:1919	Serum levels	1908:1919	Serum levels of HexNAc7Hex7DeoxyHex1NeuAc2 and HexNAc8Hex6DeoxyHex0NeuAc0	1908:1980	Serum levels of HexNAc7Hex7DeoxyHex1NeuAc2 and HexNAc8Hex6DeoxyHex0NeuAc0 were persistently increased in patients with favorable outcome, but undetectable in those with unfavorable outcome.					
35755600	15	44	theme	potential	2640:2648	arg1	applications					2650:2661	potential applications	2640:2661	potential applications	2640:2661	Further larger multicenter studies will be required to validate our findings and to determine their pathobiological value and potential applications in practice.					
35755600	2	45	theme	Scale	438:442	arg1	score					450:454	Glasgow Coma Scale [GCS] score ≤12	425:458	Glasgow Coma Scale [GCS] score ≤12	425:458	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	2	45	theme	Scale	438:442	arg1	TBI					420:422	moderate to severe TBI	401:422	moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12)	401:459	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	8	46	theme	typical	1413:1419	arg1	oligomannose					1449:1460	oligomannose	1449:1460	oligomannose	1449:1460	Findings We identified 94 N-glycans encompassing all typical structural types, including oligomannose, hybrid, and complex-type entities.					
35755600	8	46	theme	typical	1413:1419	arg1	entities					1488:1495	complex-type entities	1475:1495	complex-type entities	1475:1495	Findings We identified 94 N-glycans encompassing all typical structural types, including oligomannose, hybrid, and complex-type entities.					
35755600	8	46	theme	typical	1413:1419	arg1	hybrid					1463:1468	hybrid	1463:1468	hybrid	1463:1468	Findings We identified 94 N-glycans encompassing all typical structural types, including oligomannose, hybrid, and complex-type entities.					
35755600	8	46	theme	typical	1413:1419	arg1	types					1432:1436	all typical structural types	1409:1436	all typical structural types	1409:1436	Findings We identified 94 N-glycans encompassing all typical structural types, including oligomannose, hybrid, and complex-type entities.					
35755600	6	47	theme	Multivariate	1033:1044	arg1	analysis					1159:1166	orthogonal partial least squares discriminant analysis	1113:1166	orthogonal partial least squares discriminant analysis	1113:1166	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	6	47	theme	Multivariate	1033:1044	arg1	analysis					1100:1107	principal component analysis	1080:1107	principal component analysis	1080:1107	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	6	47	theme	Multivariate	1033:1044	arg1	techniques					1058:1067	Multivariate statistical techniques	1033:1067	Multivariate statistical techniques	1033:1067	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	2	48	theme	Glasgow	425:431	arg1	score					450:454	Glasgow Coma Scale [GCS] score ≤12	425:458	Glasgow Coma Scale [GCS] score ≤12	425:458	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	2	48	theme	Glasgow	425:431	arg1	TBI					420:422	moderate to severe TBI	401:422	moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12)	401:459	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	0	49	theme	glycome	16:22	arg1	patterns					24:31	serum glycome patterns	10:31	serum glycome patterns	10:31	Exploring serum glycome patterns after moderate to severe traumatic brain injury: A prospective pilot study.					
35755600	12	50	with	patients	2013:2020	arg1	outcome					2037:2043	favorable outcome	2027:2043	favorable outcome	2027:2043	Serum levels of HexNAc7Hex7DeoxyHex1NeuAc2 and HexNAc8Hex6DeoxyHex0NeuAc0 were persistently increased in patients with favorable outcome, but undetectable in those with unfavorable outcome.					
35755600	7	51	theme	prognostic	1339:1348	arg1	accuracy					1350:1357	prognostic accuracy	1339:1357	prognostic accuracy	1339:1357	Receiver operating characteristic analyses were used to determine prognostic accuracy.					
35755600	12	52	theme	HexNAc7Hex7DeoxyHex1NeuAc2	1924:1949	arg1	levels					1914:1919	Serum levels	1908:1919	Serum levels of HexNAc7Hex7DeoxyHex1NeuAc2 and HexNAc8Hex6DeoxyHex0NeuAc0	1908:1980	Serum levels of HexNAc7Hex7DeoxyHex1NeuAc2 and HexNAc8Hex6DeoxyHex0NeuAc0 were persistently increased in patients with favorable outcome, but undetectable in those with unfavorable outcome.					
35755600	12	53	from	those	2066:2070	arg1	undetectable					2050:2061	undetectable	2050:2061	undetectable	2050:2061	Serum levels of HexNAc7Hex7DeoxyHex1NeuAc2 and HexNAc8Hex6DeoxyHex0NeuAc0 were persistently increased in patients with favorable outcome, but undetectable in those with unfavorable outcome.					
35755600	8	54	dep	Findings	1360:1367	arg1	identified					1372:1381	identified	1372:1381	identified 94 N-glycans encompassing all typical structural types, including oligomannose, hybrid, and complex-type entities	1372:1495	Findings We identified 94 N-glycans encompassing all typical structural types, including oligomannose, hybrid, and complex-type entities.					
35755600	11	55	theme	unfavorable	1786:1796	arg1	outcome					1798:1804	unfavorable outcome	1786:1804	unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90]	1786:1865	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	11	55	theme	unfavorable	1786:1796	arg1	[AUC					1838:1841	area under the curve [AUC	1817:1841	area under the curve [AUC	1817:1841	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	11	55	theme	unfavorable	1786:1796	arg1	GOS-E≤4					1807:1813	GOS-E≤4	1807:1813	GOS-E≤4	1807:1813	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	1	56	theme	brain	318:322	arg1	TBI					332:334	TBI	332:334	TBI	332:334	Background Glycans play essential functional roles in the nervous system and their pathobiological relevance has become increasingly recognized in numerous brain disorders, but not fully explored in traumatic brain injury (TBI).					
35755600	1	56	theme	brain	318:322	arg1	injury					324:329	traumatic brain injury	308:329	traumatic brain injury (TBI)	308:335	Background Glycans play essential functional roles in the nervous system and their pathobiological relevance has become increasingly recognized in numerous brain disorders, but not fully explored in traumatic brain injury (TBI).					
35755600	12	57	from	undetectable	2050:2061	arg1	those					2066:2070	those	2066:2070	those	2066:2070	Serum levels of HexNAc7Hex7DeoxyHex1NeuAc2 and HexNAc8Hex6DeoxyHex0NeuAc0 were persistently increased in patients with favorable outcome, but undetectable in those with unfavorable outcome.					
35755600	6	58	theme	partial	1124:1130	arg1	squares					1138:1144	partial least squares	1124:1144	orthogonal partial least squares discriminant analysis	1113:1166	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	11	59	theme	area	1817:1820	arg1	outcome					1798:1804	unfavorable outcome	1786:1804	unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90]	1786:1865	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	11	59	theme	area	1817:1820	arg1	[AUC					1838:1841	area under the curve [AUC	1817:1841	area under the curve [AUC	1817:1841	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	11	60	theme	brain-specific	1635:1648	arg1	structures					1650:1659	Two brain-specific structures	1631:1659	Two brain-specific structures	1631:1659	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	11	60	theme	brain-specific	1635:1648	arg1	HexNAc5Hex4DeoxyHex0NeuAc1					1693:1718	HexNAc5Hex4DeoxyHex0NeuAc1	1693:1718	HexNAc5Hex4DeoxyHex0NeuAc1	1693:1718	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	11	60	theme	brain-specific	1635:1648	arg1	HexNAc5Hex3DeoxyHex0NeuAc0					1662:1687	HexNAc5Hex3DeoxyHex0NeuAc0	1662:1687	HexNAc5Hex3DeoxyHex0NeuAc0	1662:1687	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	5	61	theme	Glasgow	968:974	arg1	GOS-E					1000:1004	GOS-E	1000:1004	GOS-E	1000:1004	Outcome was assessed using the Glasgow Outcome Scale-Extended (GOS-E) at 12 months post-injury.					
35755600	5	61	theme	Glasgow	968:974	arg1	Scale-Extended					984:997	the Glasgow Outcome Scale-Extended	964:997	the Glasgow Outcome Scale-Extended (GOS-E)	964:1005	Outcome was assessed using the Glasgow Outcome Scale-Extended (GOS-E) at 12 months post-injury.					
35755600	7	62	theme	characteristic	1292:1305	arg1	analyses					1307:1314	characteristic analyses	1292:1314	characteristic analyses	1292:1314	Receiver operating characteristic analyses were used to determine prognostic accuracy.					
35755600	10	63	theme	influential	1594:1604	arg1	glycans					1606:1612	Four influential glycans	1589:1612	Four influential glycans	1589:1612	Four influential glycans were identified.					
35755600	8	64	theme	complex-type	1475:1486	arg1	entities					1488:1495	complex-type entities	1475:1495	complex-type entities	1475:1495	Findings We identified 94 N-glycans encompassing all typical structural types, including oligomannose, hybrid, and complex-type entities.					
35755600	2	65	theme	glycome	367:373	arg1	patterns					375:382	longitudinal glycome patterns	354:382	longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12)	354:459	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	11	66	with	patients	1772:1779	arg1	outcome					1798:1804	unfavorable outcome	1786:1804	unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90]	1786:1865	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	11	66	with	patients	1772:1779	arg1	[AUC					1838:1841	area under the curve [AUC	1817:1841	area under the curve [AUC	1817:1841	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	11	66	with	patients	1772:1779	arg1	GOS-E≤4					1807:1813	GOS-E≤4	1807:1813	GOS-E≤4	1807:1813	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	11	66	with	patients	1772:1779	arg1	AUC=0·71					1871:1878	AUC=0·71	1871:1878	AUC=0·71	1871:1878	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	0	67	dep	Exploring	0:8	arg1	study					102:106	A prospective pilot study	82:106	A prospective pilot study	82:106	Exploring serum glycome patterns after moderate to severe traumatic brain injury: A prospective pilot study.					
35755600	6	68	theme	component	1090:1098	arg1	analysis					1100:1107	principal component analysis	1080:1107	principal component analysis	1080:1107	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	1	69	theme	numerous	256:263	arg1	disorders					271:279	numerous brain disorders	256:279	numerous brain disorders	256:279	Background Glycans play essential functional roles in the nervous system and their pathobiological relevance has become increasingly recognized in numerous brain disorders, but not fully explored in traumatic brain injury (TBI).					
35755600	4	70	theme	mass	847:850	arg1	spectrometry					852:863	liquid chromatography-tandem mass spectrometry	818:863	a high-throughput liquid chromatography-tandem mass spectrometry platform	800:872	We used a high-throughput liquid chromatography-tandem mass spectrometry platform to assess serum levels of N-glycans up to 3 days after injury.					
35755600	16	71	theme	NIH	2806:2808	arg1	1R01GM112490-08					2817:2831	1R01GM112490-08	2817:2831	1R01GM112490-08	2817:2831	Funding This work was funded by the Italian Ministry of Health (grant number GR-2013-02354960), and also partially supported by a NIH grant (1R01GM112490-08).					
35755600	16	71	theme	NIH	2806:2808	arg1	grant					2810:2814	a NIH grant	2804:2814	a NIH grant (1R01GM112490-08)	2804:2832	Funding This work was funded by the Italian Ministry of Health (grant number GR-2013-02354960), and also partially supported by a NIH grant (1R01GM112490-08).					
35755600	14	72	from	phenotyping	2494:2504	arg1	TBI					2509:2511	TBI	2509:2511	TBI	2509:2511	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	14	73	theme	small	2319:2323	arg1	number					2325:2330	the relatively small number	2304:2330	the relatively small number of patients	2304:2342	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	3	74	theme	Hospital	725:732	arg1	unit					702:705	the neurosurgical unit	684:705	the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019	684:789	Methods This prospective single-center observational study included 51 adult patients with TBI (GCS ≤12) admitted to the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019.					
35755600	4	75	used	used	795:798	arg2	We					792:793	We	792:793	We	792:793	We used a high-throughput liquid chromatography-tandem mass spectrometry platform to assess serum levels of N-glycans up to 3 days after injury.					
35755600	14	76	dep	Interpretation	2237:2250	arg1	spite					2255:2259	spite	2255:2259	spite of the exploratory nature of the study and the relatively small number of patients	2255:2342	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	16	77	theme	Health	2732:2737	arg1	Ministry					2720:2727	the Italian Ministry	2708:2727	the Italian Ministry of Health (grant number GR-2013-02354960)	2708:2769	Funding This work was funded by the Italian Ministry of Health (grant number GR-2013-02354960), and also partially supported by a NIH grant (1R01GM112490-08).					
35755600	4	78	theme	liquid	818:823	arg1	spectrometry					852:863	liquid chromatography-tandem mass spectrometry	818:863	a high-throughput liquid chromatography-tandem mass spectrometry platform	800:872	We used a high-throughput liquid chromatography-tandem mass spectrometry platform to assess serum levels of N-glycans up to 3 days after injury.					
35755600	14	79	theme	study	2294:2298	arg1	nature					2280:2285	the exploratory nature	2264:2285	the exploratory nature of the study	2264:2298	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	14	79	theme	study	2294:2298	arg1	number					2325:2330	the relatively small number	2304:2330	the relatively small number of patients	2304:2342	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	14	80	theme	biomarker	2462:2470	arg1	discovery					2472:2480	biomarker discovery	2462:2480	biomarker discovery	2462:2480	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	6	81	used	used	1174:1177	arg2	techniques					1058:1067	Multivariate statistical techniques	1033:1067	Multivariate statistical techniques	1033:1067	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	6	81	used	used	1174:1177	arg2	analysis					1100:1107	principal component analysis	1080:1107	principal component analysis	1080:1107	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	6	81	used	used	1174:1177	arg2	analysis					1159:1166	orthogonal partial least squares discriminant analysis	1113:1166	orthogonal partial least squares discriminant analysis	1113:1166	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	9	82	theme	high	1508:1511	arg1	mannose					1513:1519	high mannose	1508:1519	high mannose	1508:1519	Levels of high mannose, hybrid and sialylated structures were temporally altered (p<0·05).					
35755600	16	83	theme	grant	2740:2744	arg1	GR-2013-02354960					2753:2768	grant number GR-2013-02354960	2740:2768	grant number GR-2013-02354960	2740:2768	Funding This work was funded by the Italian Ministry of Health (grant number GR-2013-02354960), and also partially supported by a NIH grant (1R01GM112490-08).					
35755600	16	83	theme	grant	2740:2744	arg1	Health					2732:2737	Health	2732:2737	Health (grant number GR-2013-02354960)	2732:2769	Funding This work was funded by the Italian Ministry of Health (grant number GR-2013-02354960), and also partially supported by a NIH grant (1R01GM112490-08).					
35755600	4	84	theme	N-glycans	900:908	arg1	levels					890:895	serum levels	884:895	serum levels of N-glycans	884:908	We used a high-throughput liquid chromatography-tandem mass spectrometry platform to assess serum levels of N-glycans up to 3 days after injury.					
35755600	6	85	theme	squares	1138:1144	arg1	analysis					1159:1166	orthogonal partial least squares discriminant analysis	1113:1166	orthogonal partial least squares discriminant analysis	1113:1166	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	3	86	theme	single-center	592:604	arg1	study					620:624	This prospective single-center observational study	575:624	This prospective single-center observational study	575:624	Methods This prospective single-center observational study included 51 adult patients with TBI (GCS ≤12) admitted to the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019.					
35755600	14	87	theme	exploratory	2268:2278	arg1	nature					2280:2285	the exploratory nature	2264:2285	the exploratory nature of the study	2264:2298	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	4	88	dep	3	916:916	arg1	to					913:914	to	913:914	to	913:914	We used a high-throughput liquid chromatography-tandem mass spectrometry platform to assess serum levels of N-glycans up to 3 days after injury.					
35755600	11	89	dep	[AUC	1838:1841	arg1	CI					1853:1854	=0·75 [95%CI 0·59-0·90	1843:1864	=0·75 [95%CI 0·59-0·90	1843:1864	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	15	90	theme	pathobiological	2614:2628	arg1	value					2630:2634	their pathobiological value	2608:2634	their pathobiological value	2608:2634	Further larger multicenter studies will be required to validate our findings and to determine their pathobiological value and potential applications in practice.					
35755600	13	91	theme	decompressive	2210:2222	arg1	craniectomy					2224:2234	decompressive craniectomy	2210:2234	decompressive craniectomy	2210:2234	Levels of HexNAc5Hex4DeoxyHex0NeuAc1 were acutely elevated in patients with mass lesions and in those requiring decompressive craniectomy.					
35755600	9	92	theme	structures	1544:1553	arg1	Levels					1498:1503	Levels	1498:1503	Levels of high mannose, hybrid and sialylated structures	1498:1553	Levels of high mannose, hybrid and sialylated structures were temporally altered (p<0·05).					
35755600	2	93	theme	long-term	548:556	arg1	outcome					558:564	long-term outcome	548:564	long-term outcome	548:564	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	15	94	from	applications	2650:2661	arg1	practice					2666:2673	practice	2666:2673	practice	2666:2673	Further larger multicenter studies will be required to validate our findings and to determine their pathobiological value and potential applications in practice.					
35755600	14	95	theme	glycomics	2437:2445	arg1	approaches					2447:2456	glycomics approaches	2437:2456	glycomics approaches	2437:2456	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	0	96	theme	brain	68:72	arg1	injury					74:79	moderate to severe traumatic brain injury	39:79	moderate to severe traumatic brain injury	39:79	Exploring serum glycome patterns after moderate to severe traumatic brain injury: A prospective pilot study.					
35755600	1	97	theme	essential	133:141	arg1	roles					154:158	essential functional roles	133:158	essential functional roles	133:158	Background Glycans play essential functional roles in the nervous system and their pathobiological relevance has become increasingly recognized in numerous brain disorders, but not fully explored in traumatic brain injury (TBI).					
35755600	13	98	with	patients	2160:2167	arg1	lesions					2179:2185	mass lesions	2174:2185	mass lesions	2174:2185	Levels of HexNAc5Hex4DeoxyHex0NeuAc1 were acutely elevated in patients with mass lesions and in those requiring decompressive craniectomy.					
35755600	9	99	gly	sialylated	1533:1542	arg1	structures					1544:1553	sialylated structures	1533:1553	sialylated structures	1533:1553	Levels of high mannose, hybrid and sialylated structures were temporally altered (p<0·05).					
35755600	6	100	theme	glycomics	1190:1198	arg1	data					1200:1203	glycomics data	1190:1203	glycomics data	1190:1203	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	11	101	theme	%	1852:1852	arg1	CI					1853:1854	=0·75 [95%CI 0·59-0·90	1843:1864	=0·75 [95%CI 0·59-0·90	1843:1864	Two brain-specific structures, HexNAc5Hex3DeoxyHex0NeuAc0 and HexNAc5Hex4DeoxyHex0NeuAc1, were substantially increased early after injury in patients with unfavorable outcome (GOS-E≤4) (area under the curve [AUC]=0·75 [95%CI 0·59-0·90] and AUC=0·71 [0·52-0·89], respectively).					
35755600	0	102	theme	prospective	84:94	arg1	study					102:106	A prospective pilot study	82:106	A prospective pilot study	82:106	Exploring serum glycome patterns after moderate to severe traumatic brain injury: A prospective pilot study.					
35755600	12	103	theme	favorable	2027:2035	arg1	outcome					2037:2043	favorable outcome	2027:2043	favorable outcome	2027:2043	Serum levels of HexNAc7Hex7DeoxyHex1NeuAc2 and HexNAc8Hex6DeoxyHex0NeuAc0 were persistently increased in patients with favorable outcome, but undetectable in those with unfavorable outcome.					
35755600	14	104	theme	initial	2394:2400	arg1	evidence					2402:2409	initial evidence	2394:2409	initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI	2394:2511	Interpretation In spite of the exploratory nature of the study and the relatively small number of patients, our results provide to the best of our knowledge initial evidence supporting the utility of glycomics approaches for biomarker discovery and patient phenotyping in TBI.					
35755600	1	105	theme	pathobiological	192:206	arg1	relevance					208:216	their pathobiological relevance	186:216	their pathobiological relevance	186:216	Background Glycans play essential functional roles in the nervous system and their pathobiological relevance has become increasingly recognized in numerous brain disorders, but not fully explored in traumatic brain injury (TBI).					
35755600	0	106	theme	serum	10:14	arg1	patterns					24:31	serum glycome patterns	10:31	serum glycome patterns	10:31	Exploring serum glycome patterns after moderate to severe traumatic brain injury: A prospective pilot study.					
35755600	13	107	theme	mass	2174:2177	arg1	lesions					2179:2185	mass lesions	2174:2185	mass lesions	2174:2185	Levels of HexNAc5Hex4DeoxyHex0NeuAc1 were acutely elevated in patients with mass lesions and in those requiring decompressive craniectomy.					
35755600	6	108	theme	statistical	1046:1056	arg1	analysis					1159:1166	orthogonal partial least squares discriminant analysis	1113:1166	orthogonal partial least squares discriminant analysis	1113:1166	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	6	108	theme	statistical	1046:1056	arg1	analysis					1100:1107	principal component analysis	1080:1107	principal component analysis	1080:1107	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	6	108	theme	statistical	1046:1056	arg1	techniques					1058:1067	Multivariate statistical techniques	1033:1067	Multivariate statistical techniques	1033:1067	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	2	109	theme	Coma	433:436	arg1	score					450:454	Glasgow Coma Scale [GCS] score ≤12	425:458	Glasgow Coma Scale [GCS] score ≤12	425:458	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	2	109	theme	Coma	433:436	arg1	TBI					420:422	moderate to severe TBI	401:422	moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12)	401:459	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	7	110	used	used	1321:1324	arg2	Receiver					1273:1280	Receiver	1273:1280	Receiver operating characteristic analyses	1273:1314	Receiver operating characteristic analyses were used to determine prognostic accuracy.					
35755600	8	111	theme	structural	1421:1430	arg1	oligomannose					1449:1460	oligomannose	1449:1460	oligomannose	1449:1460	Findings We identified 94 N-glycans encompassing all typical structural types, including oligomannose, hybrid, and complex-type entities.					
35755600	8	111	theme	structural	1421:1430	arg1	entities					1488:1495	complex-type entities	1475:1495	complex-type entities	1475:1495	Findings We identified 94 N-glycans encompassing all typical structural types, including oligomannose, hybrid, and complex-type entities.					
35755600	8	111	theme	structural	1421:1430	arg1	hybrid					1463:1468	hybrid	1463:1468	hybrid	1463:1468	Findings We identified 94 N-glycans encompassing all typical structural types, including oligomannose, hybrid, and complex-type entities.					
35755600	8	111	theme	structural	1421:1430	arg1	types					1432:1436	all typical structural types	1409:1436	all typical structural types	1409:1436	Findings We identified 94 N-glycans encompassing all typical structural types, including oligomannose, hybrid, and complex-type entities.					
35755600	2	112	theme	severe	413:418	arg1	score					450:454	Glasgow Coma Scale [GCS] score ≤12	425:458	Glasgow Coma Scale [GCS] score ≤12	425:458	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	2	112	theme	severe	413:418	arg1	TBI					420:422	moderate to severe TBI	401:422	moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12)	401:459	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	0	113	dep	severe	51:56	arg1	to					48:49	to	48:49	to	48:49	Exploring serum glycome patterns after moderate to severe traumatic brain injury: A prospective pilot study.					
35755600	13	114	theme	HexNAc5Hex4DeoxyHex0NeuAc1	2108:2133	arg1	Levels					2098:2103	Levels	2098:2103	Levels of HexNAc5Hex4DeoxyHex0NeuAc1	2098:2133	Levels of HexNAc5Hex4DeoxyHex0NeuAc1 were acutely elevated in patients with mass lesions and in those requiring decompressive craniectomy.					
35755600	0	115	theme	severe	51:56	arg1	injury					74:79	moderate to severe traumatic brain injury	39:79	moderate to severe traumatic brain injury	39:79	Exploring serum glycome patterns after moderate to severe traumatic brain injury: A prospective pilot study.					
35755600	1	116	theme	traumatic	308:316	arg1	TBI					332:334	TBI	332:334	TBI	332:334	Background Glycans play essential functional roles in the nervous system and their pathobiological relevance has become increasingly recognized in numerous brain disorders, but not fully explored in traumatic brain injury (TBI).					
35755600	1	116	theme	traumatic	308:316	arg1	injury					324:329	traumatic brain injury	308:329	traumatic brain injury (TBI)	308:335	Background Glycans play essential functional roles in the nervous system and their pathobiological relevance has become increasingly recognized in numerous brain disorders, but not fully explored in traumatic brain injury (TBI).					
35755600	2	117	from	patterns	375:382	arg1	patients					387:394	patients	387:394	patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12)	387:459	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	6	118	theme	least	1132:1136	arg1	squares					1138:1144	partial least squares	1124:1144	orthogonal partial least squares discriminant analysis	1113:1166	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	2	119	theme	[GCS	444:447	arg1	score					450:454	Glasgow Coma Scale [GCS] score ≤12	425:458	Glasgow Coma Scale [GCS] score ≤12	425:458	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	2	119	theme	[GCS	444:447	arg1	TBI					420:422	moderate to severe TBI	401:422	moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12)	401:459	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	2	120	dep	severe	413:418	arg1	to					410:411	to	410:411	to	410:411	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35755600	12	121	theme	Serum	1908:1912	arg1	levels					1914:1919	Serum levels	1908:1919	Serum levels of HexNAc7Hex7DeoxyHex1NeuAc2 and HexNAc8Hex6DeoxyHex0NeuAc0	1908:1980	Serum levels of HexNAc7Hex7DeoxyHex1NeuAc2 and HexNAc8Hex6DeoxyHex0NeuAc0 were persistently increased in patients with favorable outcome, but undetectable in those with unfavorable outcome.					
35755600	6	122	theme	orthogonal	1113:1122	arg1	analysis					1159:1166	orthogonal partial least squares discriminant analysis	1113:1166	orthogonal partial least squares discriminant analysis	1113:1166	Multivariate statistical techniques, including principal component analysis and orthogonal partial least squares discriminant analysis, were used to analyze glycomics data and define highly influential structures driving class distinction.					
35755600	5	123	theme	Outcome	976:982	arg1	GOS-E					1000:1004	GOS-E	1000:1004	GOS-E	1000:1004	Outcome was assessed using the Glasgow Outcome Scale-Extended (GOS-E) at 12 months post-injury.					
35755600	5	123	theme	Outcome	976:982	arg1	Scale-Extended					984:997	the Glasgow Outcome Scale-Extended	964:997	the Glasgow Outcome Scale-Extended (GOS-E)	964:1005	Outcome was assessed using the Glasgow Outcome Scale-Extended (GOS-E) at 12 months post-injury.					
35755600	3	124	theme	neurosurgical	688:700	arg1	unit					702:705	the neurosurgical unit	684:705	the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019	684:789	Methods This prospective single-center observational study included 51 adult patients with TBI (GCS ≤12) admitted to the neurosurgical unit of the University Hospital of Pecs, Pecs, Hungary, between June 2018 and April 2019.					
35755600	2	125	theme	longitudinal	354:365	arg1	patterns					375:382	longitudinal glycome patterns	354:382	longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12)	354:459	We investigated longitudinal glycome patterns in patients with moderate to severe TBI (Glasgow Coma Scale [GCS] score ≤12) to characterize glyco-biomarker signatures and their relation to clinical features and long-term outcome.					
35964397	9	0	theme	simultaneous	1954:1965	arg1	remediation					1967:1977	simultaneous remediation	1954:1977	simultaneous remediation	1954:1977	Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil.					
35964397	4	1	theme	immobilized	554:564	arg1	biocomposite					566:577	the immobilized biocomposite	550:577	the immobilized biocomposite	550:577	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	6	2	theme	Cr	1166:1167	arg1	proportion					1169:1178	the residual Cr proportion	1153:1178	the residual Cr proportion (49.04%)	1153:1187	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	6	2	theme	Cr	1166:1167	arg1	%					1186:1186	49.04%	1181:1186	49.04%	1181:1186	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	1	3	theme	in-situ	193:199	arg1	Cr					211:212	in-situ remediate Cr(VI)-contaminated soil	193:234	in-situ remediate Cr(VI)-contaminated soil	193:234	Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed.					
35964397	7	4	theme	immobilizers	1373:1384	arg1	synergies					1350:1358	the synergies	1346:1358	the synergies of CRB-7 and immobilizers (HA, VE and SA)	1346:1400	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	2	5	theme	novel	308:312	arg1	B-HA-VE-SA					340:349	B-HA-VE-SA	340:349	B-HA-VE-SA	340:349	Herein, a novel immobilized biocomposite (B-HA-VE-SA) was successfully synthesized by embedding a efficient strain Bacillus sp.					
35964397	2	5	theme	novel	308:312	arg1	biocomposite					326:337	a novel immobilized biocomposite	306:337	a novel immobilized biocomposite (B-HA-VE-SA)	306:350	Herein, a novel immobilized biocomposite (B-HA-VE-SA) was successfully synthesized by embedding a efficient strain Bacillus sp.					
35964397	7	6	theme	composition	1555:1565	arg1	evolution					1522:1530	the evolution	1518:1530	the evolution of microbial community composition and functional pathways	1518:1589	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	4	7	theme	-contaminated	600:612	arg1	soil					614:617	-contaminated soil	600:617	Cr(VI)-contaminated soil	594:617	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	8	8	theme	root	1824:1827	arg1	parts					1840:1844	root and aerial parts	1824:1844	root and aerial parts	1824:1844	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	8	9	theme	plant-growth-promoting	1655:1676	arg1	effects					1776:1782	the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects	1651:1782	the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7	1651:1875	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	7	10	theme	microbial	1535:1543	arg1	composition					1555:1565	microbial community composition	1535:1565	microbial community composition	1535:1565	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	6	11	theme	carrier	1223:1229	arg1	effects					1231:1237	carrier effects	1223:1237	carrier effects	1223:1237	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	5	12	theme	multiple	827:834	arg1	genes					836:840	multiple genes	827:840	multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance	827:918	Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance.					
35964397	4	13	theme	Cr	682:683	arg1	whole-genome					659:670	the whole-genome	655:670	the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response	655:772	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	4	14	dep	Cr	682:683	arg1	detoxification					689:702	detoxification	689:702	Cr(VI) detoxification	682:702	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	4	14	dep	Cr	682:683	arg1	VI					685:686	VI	685:686	VI	685:686	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	8	15	theme	free	1850:1853	arg1	CRB-7					1871:1875	free and immobilized CRB-7	1850:1875	free and immobilized CRB-7	1850:1875	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	4	16	dep	Cr	594:595	arg1	soil					614:617	-contaminated soil	600:617	Cr(VI)-contaminated soil	594:617	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	4	16	dep	Cr	594:595	arg1	VI					597:598	VI	597:598	VI	597:598	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	7	17	dep	CRB-7	1363:1367	arg1	VE					1391:1392	VE	1391:1392	VE	1391:1392	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	7	17	dep	CRB-7	1363:1367	arg1	HA					1387:1388	HA	1387:1388	HA	1387:1388	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	7	17	dep	CRB-7	1363:1367	arg1	SA					1398:1399	SA	1398:1399	SA	1398:1399	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	6	18	theme	removal	1121:1127	arg1	efficiency					1129:1138	removal efficiency	1121:1138	Cr(VI) removal efficiency (96.18%)	1114:1147	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	8	19	theme	immobilized	1859:1869	arg1	CRB-7					1871:1875	free and immobilized CRB-7	1850:1875	free and immobilized CRB-7	1850:1875	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	4	20	dep	performance	509:519	arg1	The					505:507	The	505:507	The	505:507	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	4	21	theme	CRB-7	675:679	arg1	whole-genome					659:670	the whole-genome	655:670	the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response	655:772	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	6	22	theme	embedded	941:948	arg1	biocomposites					956:968	embedded CRB-7 biocomposites	941:968	embedded CRB-7 biocomposites	941:968	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	4	23	theme	regulation	726:735	arg1	whole-genome					659:670	the whole-genome	655:670	the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response	655:772	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	0	24	theme	microbial-humic	84:98	arg1	biocomposite					138:149	microbial-humic acid-vermiculite-alginate immobilized biocomposite	84:149	microbial-humic acid-vermiculite-alginate immobilized biocomposite	84:149	Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite.					
35964397	5	25	contain	contains	818:825	arg1	CRB-7					812:816	CRB-7	812:816	CRB-7	812:816	Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance.					
35964397	5	25	contain	contains	818:825	arg2	genes					836:840	multiple genes	827:840	multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance	827:918	Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance.					
35964397	5	26	theme	Genomic	775:781	arg1	annotation					783:792	Genomic annotation	775:792	Genomic annotation	775:792	Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance.					
35964397	7	27	theme	pathways	1582:1589	arg1	evolution					1522:1530	the evolution	1518:1530	the evolution of microbial community composition and functional pathways	1518:1589	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	8	28	from	%	1796:1796	arg1	parts					1840:1844	root and aerial parts	1824:1844	root and aerial parts	1824:1844	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	6	29	dep	soil	1018:1021	arg1	VI					1011:1012	VI	1011:1012	VI	1011:1012	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	7	30	theme	soil	1444:1447	arg1	activities					1456:1465	soil enzyme activities	1444:1465	soil enzyme activities	1444:1465	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	5	31	theme	other	896:900	arg1	metals					902:907	other metals	896:907	other metals	896:907	Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance.					
35964397	6	32	theme	Cr	1008:1009	arg1	reduction					995:1003	more effective reduction	980:1003	more effective reduction of Cr	980:1009	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	9	33	theme	microecological	1980:1994	arg1	improvement					1996:2006	microecological improvement	1980:2006	microecological improvement	1980:2006	Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil.					
35964397	0	34	theme	soil	43:46	arg1	remediation					48:58	Cr(VI)-contaminated soil remediation	23:58	Cr(VI)-contaminated soil remediation	23:58	Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite.					
35964397	4	35	theme	crop	753:756	arg1	response					765:772	subsequent crop growth response	742:772	subsequent crop growth response	742:772	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	5	36	dep	Cr	857:858	arg1	resistance					909:918	resistance	909:918	resistance	909:918	Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance.					
35964397	5	36	dep	Cr	857:858	arg1	tolerance					864:872	tolerance	864:872	tolerance	864:872	Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance.					
35964397	5	36	dep	Cr	857:858	arg1	VI					860:861	VI	860:861	VI	860:861	Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance.					
35964397	7	37	dep	microecology	1430:1441	arg1	diversity					1491:1499	diversity	1491:1499	diversity	1491:1499	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	7	37	dep	microecology	1430:1441	arg1	activities					1456:1465	soil enzyme activities	1444:1465	soil enzyme activities	1444:1465	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	7	37	dep	microecology	1430:1441	arg1	quantity					1478:1485	microbial quantity	1468:1485	microbial quantity	1468:1485	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	3	38	with	CRB-7	426:430	arg1	acid					443:446	humic acid	437:446	humic acid (HA)	437:451	CRB-7 with humic acid (HA) combined vermiculite (VE) and sodium alginate (SA).					
35964397	3	38	with	CRB-7	426:430	arg1	HA					449:450	HA	449:450	HA	449:450	CRB-7 with humic acid (HA) combined vermiculite (VE) and sodium alginate (SA).					
35964397	6	39	theme	bioremediation	1309:1322	arg1	process					1324:1330	the bioremediation process	1305:1330	the bioremediation process	1305:1330	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	6	40	theme	free	1049:1052	arg1	treatment					1060:1068	free CRB-7 treatment	1049:1068	free CRB-7 treatment	1049:1068	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	2	41	theme	Bacillus	413:420	arg1	sp					422:423	Bacillus sp	413:423	a efficient strain Bacillus sp	394:423	Herein, a novel immobilized biocomposite (B-HA-VE-SA) was successfully synthesized by embedding a efficient strain Bacillus sp.					
35964397	4	42	theme	response	765:772	arg1	whole-genome					659:670	the whole-genome	655:670	the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response	655:772	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	3	43	theme	sodium	483:488	arg1	alginate					490:497	sodium alginate	483:497	sodium alginate (SA)	483:502	CRB-7 with humic acid (HA) combined vermiculite (VE) and sodium alginate (SA).					
35964397	3	43	theme	sodium	483:488	arg1	SA					500:501	SA	500:501	SA	500:501	CRB-7 with humic acid (HA) combined vermiculite (VE) and sodium alginate (SA).					
35964397	1	44	theme	strengthening	249:261	arg1	methods					263:269	further strengthening methods	241:269	further strengthening methods	241:269	Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed.					
35964397	9	45	theme	-contaminated	2038:2050	arg1	soil					2052:2055	-contaminated soil	2038:2055	Cr(VI)-contaminated soil	2032:2055	Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil.					
35964397	8	46	theme	pot	1606:1608	arg1	Brassica					1623:1630	Brassica	1623:1630	Brassica	1623:1630	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	8	46	theme	pot	1606:1608	arg1	experiments					1610:1620	pot experiments	1606:1620	pot experiments (Brassica napus L.)	1606:1640	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	7	47	theme	CRB-7	1363:1367	arg1	synergies					1350:1358	the synergies	1346:1358	the synergies of CRB-7 and immobilizers (HA, VE and SA)	1346:1400	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	8	48	dep	effects	1776:1782	arg1	%					1796:1796	19.47-91.09%	1785:1796	19.47-91.09%	1785:1796	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	8	48	dep	effects	1776:1782	arg1	%					1813:1813	29.11-89.80% lower	1802:1819	29.11-89.80% lower in root and aerial parts	1802:1844	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	4	49	theme	biocomposite	566:577	arg1	performance					509:519	performance	509:519	performance	509:519	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	4	49	theme	biocomposite	566:577	arg1	mechanism					537:545	enhancement mechanism	525:545	enhancement mechanism	525:545	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	6	50	theme	CRB-7	950:954	arg1	biocomposites					956:968	embedded CRB-7 biocomposites	941:968	embedded CRB-7 biocomposites	941:968	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	6	51	theme	highest	1106:1112	arg1	Cr					1114:1115	Cr(VI) removal efficiency (96.18%)	1114:1147	Cr(VI) removal efficiency (96.18%)	1114:1147	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	6	51	theme	highest	1106:1112	arg1	%					1146:1146	96.18%	1141:1146	96.18%	1141:1146	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	6	52	theme	residual	1157:1164	arg1	proportion					1169:1178	the residual Cr proportion	1153:1178	the residual Cr proportion (49.04%)	1153:1187	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	6	52	theme	residual	1157:1164	arg1	%					1186:1186	49.04%	1181:1186	49.04%	1181:1186	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	2	53	theme	immobilized	314:324	arg1	B-HA-VE-SA					340:349	B-HA-VE-SA	340:349	B-HA-VE-SA	340:349	Herein, a novel immobilized biocomposite (B-HA-VE-SA) was successfully synthesized by embedding a efficient strain Bacillus sp.					
35964397	2	53	theme	immobilized	314:324	arg1	biocomposite					326:337	a novel immobilized biocomposite	306:337	a novel immobilized biocomposite (B-HA-VE-SA)	306:350	Herein, a novel immobilized biocomposite (B-HA-VE-SA) was successfully synthesized by embedding a efficient strain Bacillus sp.					
35964397	1	54	theme	remediate	201:209	arg1	Cr					211:212	in-situ remediate Cr(VI)-contaminated soil	193:234	in-situ remediate Cr(VI)-contaminated soil	193:234	Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed.					
35964397	7	55	theme	community	1545:1553	arg1	composition					1555:1565	microbial community composition	1535:1565	microbial community composition	1535:1565	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	6	56	theme	nutrient	1240:1247	arg1	sustained-release					1249:1265	nutrient sustained-release	1240:1265	nutrient sustained-release	1240:1265	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	9	57	dep	Cr	2032:2033	arg1	VI					2035:2036	VI	2035:2036	VI	2035:2036	Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil.					
35964397	9	57	dep	Cr	2032:2033	arg1	soil					2052:2055	-contaminated soil	2038:2055	Cr(VI)-contaminated soil	2032:2055	Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil.					
35964397	6	58	theme	multiple	1193:1200	arg1	mechanisms					1202:1211	multiple mechanisms	1193:1211	multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect	1193:1294	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	6	58	theme	multiple	1193:1200	arg1	effects					1231:1237	carrier effects	1223:1237	carrier effects	1223:1237	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	6	58	theme	multiple	1193:1200	arg1	sustained-release					1249:1265	nutrient sustained-release	1240:1265	nutrient sustained-release	1240:1265	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	6	58	theme	multiple	1193:1200	arg1	effect					1289:1294	electron-shuttle effect	1272:1294	electron-shuttle effect	1272:1294	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	6	59	theme	effective	985:993	arg1	reduction					995:1003	more effective reduction	980:1003	more effective reduction of Cr	980:1009	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	8	60	theme	CRB-7	1871:1875	arg1	effects					1776:1782	the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects	1651:1782	the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7	1651:1875	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	6	61	dep	Cr	1114:1115	arg1	efficiency					1129:1138	removal efficiency	1121:1138	Cr(VI) removal efficiency (96.18%)	1114:1147	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	6	61	dep	Cr	1114:1115	arg1	the					1102:1104	the	1102:1104	the	1102:1104	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	6	61	dep	Cr	1114:1115	arg1	VI					1117:1118	VI	1117:1118	VI	1117:1118	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	0	62	theme	safe	64:67	arg1	utilization					69:79	safe utilization	64:79	safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite	64:149	Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite.					
35964397	8	63	theme	higher	1709:1714	arg1	rate					1729:1732	12.00-18.00% and 43.82-69.00% higher in emergence rate	1679:1732	12.00-18.00% and 43.82-69.00% higher in emergence rate	1679:1732	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	1	64	theme	efficient	171:179	arg1	Bioreduction					152:163	Bioreduction	152:163	Bioreduction	152:163	Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed.					
35964397	1	64	theme	efficient	171:179	arg1	approach					181:188	an efficient approach	168:188	an efficient approach to in-situ remediate Cr(VI)-contaminated soil	168:234	Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed.					
35964397	7	65	theme	microbial	1468:1476	arg1	quantity					1478:1485	microbial quantity	1468:1485	microbial quantity	1468:1485	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	0	66	theme	immobilized	126:136	arg1	biocomposite					138:149	microbial-humic acid-vermiculite-alginate immobilized biocomposite	84:149	microbial-humic acid-vermiculite-alginate immobilized biocomposite	84:149	Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite.					
35964397	8	67	dep	plant-growth-promoting	1655:1676	arg1	biomass					1738:1744	biomass	1738:1744	biomass	1738:1744	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	8	67	dep	plant-growth-promoting	1655:1676	arg1	rate					1729:1732	12.00-18.00% and 43.82-69.00% higher in emergence rate	1679:1732	12.00-18.00% and 43.82-69.00% higher in emergence rate	1679:1732	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	6	68	theme	electron-shuttle	1272:1287	arg1	effect					1289:1294	electron-shuttle effect	1272:1294	electron-shuttle effect	1272:1294	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	4	69	theme	microecological	710:724	arg1	regulation					726:735	soil microecological regulation	705:735	soil microecological regulation	705:735	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	0	70	theme	acid-vermiculite-alginate	100:124	arg1	biocomposite					138:149	microbial-humic acid-vermiculite-alginate immobilized biocomposite	84:149	microbial-humic acid-vermiculite-alginate immobilized biocomposite	84:149	Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite.					
35964397	9	71	theme	B-HA-VE-SA	1940:1949	arg1	superiority					1925:1935	the superiority	1921:1935	the superiority of B-HA-VE-SA	1921:1949	Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil.					
35964397	5	72	dep	Cr	875:876	arg1	reduction					882:890	reduction	882:890	Cr(VI) reduction	875:890	Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance.					
35964397	5	72	dep	Cr	875:876	arg1	VI					878:879	VI	878:879	VI	878:879	Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance.					
35964397	2	73	theme	efficient	396:404	arg1	strain					406:411	a efficient strain	394:411	a efficient strain Bacillus sp	394:423	Herein, a novel immobilized biocomposite (B-HA-VE-SA) was successfully synthesized by embedding a efficient strain Bacillus sp.					
35964397	4	74	theme	enhancement	525:535	arg1	mechanism					537:545	enhancement mechanism	525:545	enhancement mechanism	525:545	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	9	75	theme	Cr	2032:2033	arg1	improvement					1996:2006	microecological improvement	1980:2006	microecological improvement	1980:2006	Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil.					
35964397	9	75	theme	Cr	2032:2033	arg1	utilization					2017:2027	safe utilization	2012:2027	safe utilization	2012:2027	Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil.					
35964397	9	75	theme	Cr	2032:2033	arg1	remediation					1967:1977	simultaneous remediation	1954:1977	simultaneous remediation	1954:1977	Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil.					
35964397	8	76	dep	Brassica	1623:1630	arg1	L.					1638:1639	Brassica napus L.	1623:1639	Brassica napus L.	1623:1639	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	3	77	theme	humic	437:441	arg1	acid					443:446	humic acid	437:446	humic acid (HA)	437:451	CRB-7 with humic acid (HA) combined vermiculite (VE) and sodium alginate (SA).					
35964397	3	77	theme	humic	437:441	arg1	HA					449:450	HA	449:450	HA	449:450	CRB-7 with humic acid (HA) combined vermiculite (VE) and sodium alginate (SA).					
35964397	0	78	theme	Cr	23:24	arg1	remediation					48:58	Cr(VI)-contaminated soil remediation	23:58	Cr(VI)-contaminated soil remediation	23:58	Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite.					
35964397	2	79	dep	strain	406:411	arg1	sp					422:423	Bacillus sp	413:423	a efficient strain Bacillus sp	394:423	Herein, a novel immobilized biocomposite (B-HA-VE-SA) was successfully synthesized by embedding a efficient strain Bacillus sp.					
35964397	8	80	theme	Cr-accumulation-reducing	1751:1774	arg1	effects					1776:1782	the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects	1651:1782	the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7	1651:1875	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	0	81	dep	remediation	48:58	arg1	VI					26:27	VI	26:27	VI	26:27	Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite.					
35964397	7	82	theme	enzyme	1449:1454	arg1	activities					1456:1465	soil enzyme activities	1444:1465	soil enzyme activities	1444:1465	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	7	83	theme	functional	1571:1580	arg1	pathways					1582:1589	functional pathways	1571:1589	functional pathways	1571:1589	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	1	84	dep	Cr	211:212	arg1	VI					214:215	VI	214:215	VI	214:215	Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed.					
35964397	1	84	dep	Cr	211:212	arg1	soil					231:234	-contaminated soil	217:234	in-situ remediate Cr(VI)-contaminated soil	193:234	Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed.					
35964397	4	85	theme	soil	705:708	arg1	regulation					726:735	soil microecological regulation	705:735	soil microecological regulation	705:735	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	0	86	theme	-contaminated	29:41	arg1	remediation					48:58	Cr(VI)-contaminated soil remediation	23:58	Cr(VI)-contaminated soil remediation	23:58	Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite.					
35964397	8	87	theme	aerial	1833:1838	arg1	parts					1840:1844	root and aerial parts	1824:1844	root and aerial parts	1824:1844	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	8	88	from	%	1813:1813	arg1	parts					1840:1844	root and aerial parts	1824:1844	root and aerial parts	1824:1844	Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7.					
35964397	9	89	theme	safe	2012:2015	arg1	utilization					2017:2027	safe utilization	2012:2027	safe utilization	2012:2027	Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil.					
35964397	7	90	theme	soil	1425:1428	arg1	microecology					1430:1441	soil microecology	1425:1441	soil microecology (soil enzyme activities, microbial quantity and diversity)	1425:1500	Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways.					
35964397	6	91	theme	CRB-7	1054:1058	arg1	treatment					1060:1068	free CRB-7 treatment	1049:1068	free CRB-7 treatment	1049:1068	Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process.					
35964397	4	92	theme	subsequent	742:751	arg1	response					765:772	subsequent crop growth response	742:772	subsequent crop growth response	742:772	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	1	93	theme	-contaminated	217:229	arg1	soil					231:234	-contaminated soil	217:234	in-situ remediate Cr(VI)-contaminated soil	193:234	Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed.					
35964397	4	94	theme	growth	758:763	arg1	response					765:772	subsequent crop growth response	742:772	subsequent crop growth response	742:772	The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response.					
35964397	1	95	theme	further	241:247	arg1	methods					263:269	further strengthening methods	241:269	further strengthening methods	241:269	Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed.					
35840240	6	0	dep	determining	1042:1052	arg1	ii					1038:1039	ii	1038:1039	ii	1038:1039	The applied cooking time determined macronutrient digestibility in the powders by (i) affecting the susceptibility of ICC to in vitro digestion, and (ii) determining the microstructural properties of WL.					
35840240	4	1	theme	precooked	650:658	arg1	WL					682:683	WL	682:683	WL	682:683	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35840240	4	1	theme	precooked	650:658	arg1	powders					673:679	precooked whole lentil powders	650:679	precooked whole lentil powders (WL)	650:684	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35840240	7	2	theme	stronger	1125:1132	arg1	attenuation					1134:1144	a stronger attenuation	1123:1144	a stronger attenuation of amylolysis	1123:1158	Freeze-dried ICC powder showed a stronger attenuation of amylolysis compared air-dried ICC.					
35840240	8	3	theme	higher	1277:1282	arg1	ingredients					1265:1275	innovative food ingredients	1249:1275	innovative food ingredients higher in fiber but lower in starch compared to ICC	1249:1327	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	8	3	theme	higher	1277:1282	arg1	potential					1236:1244	an important potential	1223:1244	an important potential	1223:1244	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	6	4	theme	macronutrient	924:936	arg1	digestibility					938:950	macronutrient digestibility	924:950	macronutrient digestibility	924:950	The applied cooking time determined macronutrient digestibility in the powders by (i) affecting the susceptibility of ICC to in vitro digestion, and (ii) determining the microstructural properties of WL.					
35840240	6	5	theme	in	1013:1014	arg1	digestion					1022:1030	in vitro digestion	1013:1030	in vitro digestion	1013:1030	The applied cooking time determined macronutrient digestibility in the powders by (i) affecting the susceptibility of ICC to in vitro digestion, and (ii) determining the microstructural properties of WL.					
35840240	0	6	from	Effect	0:5	arg1	digestibility					66:78	protein digestibility	58:78	protein digestibility	58:78	Effect of manufacturing conditions on in vitro starch and protein digestibility of (cellular) lentil-based ingredients.					
35840240	0	6	from	Effect	0:5	arg1	starch					47:52	in vitro starch	38:52	in vitro starch	38:52	Effect of manufacturing conditions on in vitro starch and protein digestibility of (cellular) lentil-based ingredients.					
35840240	3	7	dep	conditions	373:382	arg1	cooking					385:391	cooking	385:391	cooking time, application of cell isolation, and drying method	385:446	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	0	8	dep	lentil-based	94:105	arg1	cellular					84:91	cellular	84:91	cellular	84:91	Effect of manufacturing conditions on in vitro starch and protein digestibility of (cellular) lentil-based ingredients.					
35840240	3	9	dep	in	489:490	arg1	vitro					492:496	vitro	492:496	vitro	492:496	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	6	10	dep	affecting	974:982	arg1	i					971:971	i	971:971	i	971:971	The applied cooking time determined macronutrient digestibility in the powders by (i) affecting the susceptibility of ICC to in vitro digestion, and (ii) determining the microstructural properties of WL.					
35840240	6	11	theme	microstructural	1058:1072	arg1	properties					1074:1083	the microstructural properties	1054:1083	the microstructural properties of WL	1054:1089	The applied cooking time determined macronutrient digestibility in the powders by (i) affecting the susceptibility of ICC to in vitro digestion, and (ii) determining the microstructural properties of WL.					
35840240	6	12	theme	WL	1088:1089	arg1	properties					1074:1083	the microstructural properties	1054:1083	the microstructural properties of WL	1054:1089	The applied cooking time determined macronutrient digestibility in the powders by (i) affecting the susceptibility of ICC to in vitro digestion, and (ii) determining the microstructural properties of WL.					
35840240	3	13	theme	protein	509:515	arg1	digestibility					517:529	protein digestibility	509:529	protein digestibility	509:529	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	4	14	theme	powders	673:679	arg1	production					636:645	the production	632:645	the production of precooked whole lentil powders (WL)	632:684	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35840240	0	15	theme	lentil-based	94:105	arg1	ingredients					107:117	(cellular) lentil-based ingredients	83:117	(cellular) lentil-based ingredients	83:117	Effect of manufacturing conditions on in vitro starch and protein digestibility of (cellular) lentil-based ingredients.					
35840240	8	16	theme	WL	1207:1208	arg1	powders					1210:1216	WL powders	1207:1216	WL powders	1207:1216	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	5	17	theme	raw-milled	863:872	arg1	flour					881:885	raw-milled lentil flour	863:885	raw-milled lentil flour	863:885	In a model food system (heat-treated suspension), starch and protein digestion were significantly attenuated for both WL and ICC compared to raw-milled lentil flour.					
35840240	5	18	theme	food	733:736	arg1	system					738:743	a model food system	725:743	a model food system (heat-treated suspension)	725:769	In a model food system (heat-treated suspension), starch and protein digestion were significantly attenuated for both WL and ICC compared to raw-milled lentil flour.					
35840240	5	18	theme	food	733:736	arg1	suspension					759:768	heat-treated suspension	746:768	heat-treated suspension	746:768	In a model food system (heat-treated suspension), starch and protein digestion were significantly attenuated for both WL and ICC compared to raw-milled lentil flour.					
35840240	3	19	dep	composition	456:466	arg1	the					452:454	the	452:454	the	452:454	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	20	theme	isolation	419:427	arg1	time					393:396	time	393:396	time	393:396	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	20	theme	isolation	419:427	arg1	method					441:446	drying method	434:446	drying method	434:446	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	20	theme	isolation	419:427	arg1	application					399:409	application	399:409	application of cell isolation	399:427	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	7	21	theme	ICC	1105:1107	arg1	powder					1109:1114	Freeze-dried ICC powder	1092:1114	Freeze-dried ICC powder	1092:1114	Freeze-dried ICC powder showed a stronger attenuation of amylolysis compared air-dried ICC.					
35840240	8	22	theme	important	1226:1234	arg1	ingredients					1265:1275	innovative food ingredients	1249:1275	innovative food ingredients higher in fiber but lower in starch compared to ICC	1249:1327	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	8	22	theme	important	1226:1234	arg1	potential					1236:1244	an important potential	1223:1244	an important potential	1223:1244	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	4	23	theme	isolation	706:714	arg1	step					716:719	a cellular isolation step	695:719	a cellular isolation step	695:719	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35840240	2	24	from	effect	213:218	arg1	properties					255:264	the properties	251:264	the properties of those powders	251:281	However, the effect of manufacturing conditions on the properties of those powders remained unknown.					
35840240	5	25	theme	protein	783:789	arg1	digestion					791:799	protein digestion	783:799	protein digestion	783:799	In a model food system (heat-treated suspension), starch and protein digestion were significantly attenuated for both WL and ICC compared to raw-milled lentil flour.					
35840240	3	26	theme	conditions	373:382	arg1	effect					340:345	the effect	336:345	the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders	336:547	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	27	theme	in	489:490	arg1	starch					498:503	in vitro starch	489:503	in vitro starch	489:503	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	28	theme	lentil	534:539	arg1	powders					541:547	lentil powders	534:547	lentil powders	534:547	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	6	29	theme	cooking	900:906	arg1	time					908:911	The applied cooking time	888:911	The applied cooking time	888:911	The applied cooking time determined macronutrient digestibility in the powders by (i) affecting the susceptibility of ICC to in vitro digestion, and (ii) determining the microstructural properties of WL.					
35840240	3	30	theme	powders	541:547	arg1	composition					456:466	composition	456:466	composition	456:466	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	30	theme	powders	541:547	arg1	digestibility					517:529	protein digestibility	509:529	protein digestibility	509:529	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	30	theme	powders	541:547	arg1	microstructure					469:482	microstructure	469:482	microstructure	469:482	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	30	theme	powders	541:547	arg1	starch					498:503	in vitro starch	489:503	in vitro starch	489:503	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	4	31	theme	cellular	697:704	arg1	step					716:719	a cellular isolation step	695:719	a cellular isolation step	695:719	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35840240	3	32	theme	cell	414:417	arg1	isolation					419:427	cell isolation	414:427	cell isolation	414:427	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	0	33	theme	conditions	24:33	arg1	Effect					0:5	Effect	0:5	Effect of manufacturing conditions on in vitro starch and protein digestibility of (cellular) lentil-based ingredients.	0:118	Effect of manufacturing conditions on in vitro starch and protein digestibility of (cellular) lentil-based ingredients.					
35840240	6	34	theme	applied	892:898	arg1	time					908:911	The applied cooking time	888:911	The applied cooking time	888:911	The applied cooking time determined macronutrient digestibility in the powders by (i) affecting the susceptibility of ICC to in vitro digestion, and (ii) determining the microstructural properties of WL.					
35840240	5	35	theme	lentil	874:879	arg1	flour					881:885	raw-milled lentil flour	863:885	raw-milled lentil flour	863:885	In a model food system (heat-treated suspension), starch and protein digestion were significantly attenuated for both WL and ICC compared to raw-milled lentil flour.					
35840240	8	36	theme	food	1260:1263	arg1	ingredients					1265:1275	innovative food ingredients	1249:1275	innovative food ingredients higher in fiber but lower in starch compared to ICC	1249:1327	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	8	36	theme	food	1260:1263	arg1	potential					1236:1244	an important potential	1223:1244	an important potential	1223:1244	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	3	37	theme	manufacturing	359:371	arg1	conditions					373:382	specific manufacturing conditions	350:382	specific manufacturing conditions (cooking time, application of cell isolation, and drying method)	350:447	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	0	38	theme	ingredients	107:117	arg1	digestibility					66:78	protein digestibility	58:78	protein digestibility	58:78	Effect of manufacturing conditions on in vitro starch and protein digestibility of (cellular) lentil-based ingredients.					
35840240	0	38	theme	ingredients	107:117	arg1	starch					47:52	in vitro starch	38:52	in vitro starch	38:52	Effect of manufacturing conditions on in vitro starch and protein digestibility of (cellular) lentil-based ingredients.					
35840240	2	39	theme	powders	275:281	arg1	properties					255:264	the properties	251:264	the properties of those powders	251:281	However, the effect of manufacturing conditions on the properties of those powders remained unknown.					
35840240	1	40	theme	different	183:191	arg1	foods					193:197	different foods	183:197	different foods	183:197	(Cellular) pulse powders are being proposed as ingredients for different foods.					
35840240	0	41	theme	in	38:39	arg1	starch					47:52	in vitro starch	38:52	in vitro starch	38:52	Effect of manufacturing conditions on in vitro starch and protein digestibility of (cellular) lentil-based ingredients.					
35840240	6	42	dep	in	1013:1014	arg1	vitro					1016:1020	vitro	1016:1020	vitro	1016:1020	The applied cooking time determined macronutrient digestibility in the powders by (i) affecting the susceptibility of ICC to in vitro digestion, and (ii) determining the microstructural properties of WL.					
35840240	6	43	theme	ICC	1006:1008	arg1	susceptibility					988:1001	the susceptibility	984:1001	the susceptibility of ICC to in vitro digestion	984:1030	The applied cooking time determined macronutrient digestibility in the powders by (i) affecting the susceptibility of ICC to in vitro digestion, and (ii) determining the microstructural properties of WL.					
35840240	3	44	from	effect	340:345	arg1	composition					456:466	composition	456:466	composition	456:466	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	44	from	effect	340:345	arg1	microstructure					469:482	microstructure	469:482	microstructure	469:482	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	44	from	effect	340:345	arg1	starch					498:503	in vitro starch	489:503	in vitro starch	489:503	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	44	from	effect	340:345	arg1	digestibility					517:529	protein digestibility	509:529	protein digestibility	509:529	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	45	theme	specific	350:357	arg1	conditions					373:382	specific manufacturing conditions	350:382	specific manufacturing conditions (cooking time, application of cell isolation, and drying method)	350:447	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	3	46	theme	drying	434:439	arg1	method					441:446	drying method	434:446	drying method	434:446	Therefore, this study investigated the effect of specific manufacturing conditions (cooking time, application of cell isolation, and drying method) on the composition, microstructure, and in vitro starch and protein digestibility of lentil powders.					
35840240	5	47	theme	heat-treated	746:757	arg1	system					738:743	a model food system	725:743	a model food system (heat-treated suspension)	725:769	In a model food system (heat-treated suspension), starch and protein digestion were significantly attenuated for both WL and ICC compared to raw-milled lentil flour.					
35840240	5	47	theme	heat-treated	746:757	arg1	suspension					759:768	heat-treated suspension	746:768	heat-treated suspension	746:768	In a model food system (heat-treated suspension), starch and protein digestion were significantly attenuated for both WL and ICC compared to raw-milled lentil flour.					
35840240	0	48	dep	in	38:39	arg1	vitro					41:45	vitro	41:45	vitro	41:45	Effect of manufacturing conditions on in vitro starch and protein digestibility of (cellular) lentil-based ingredients.					
35840240	4	49	theme	lentil	666:671	arg1	WL					682:683	WL	682:683	WL	682:683	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35840240	4	49	theme	lentil	666:671	arg1	powders					673:679	precooked whole lentil powders	650:679	precooked whole lentil powders (WL)	650:684	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35840240	5	50	theme	model	727:731	arg1	system					738:743	a model food system	725:743	a model food system (heat-treated suspension)	725:769	In a model food system (heat-treated suspension), starch and protein digestion were significantly attenuated for both WL and ICC compared to raw-milled lentil flour.					
35840240	5	50	theme	model	727:731	arg1	suspension					759:768	heat-treated suspension	746:768	heat-treated suspension	746:768	In a model food system (heat-treated suspension), starch and protein digestion were significantly attenuated for both WL and ICC compared to raw-milled lentil flour.					
35840240	8	51	contain	have	1218:1221	arg1	powders					1210:1216	WL powders	1207:1216	WL powders	1207:1216	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	8	51	contain	have	1218:1221	arg2	potential					1236:1244	an important potential	1223:1244	an important potential	1223:1244	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	8	51	contain	have	1218:1221	arg2	ingredients					1265:1275	innovative food ingredients	1249:1275	innovative food ingredients higher in fiber but lower in starch compared to ICC	1249:1327	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	4	52	theme	whole	660:664	arg1	WL					682:683	WL	682:683	WL	682:683	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35840240	4	52	theme	whole	660:664	arg1	powders					673:679	precooked whole lentil powders	650:679	precooked whole lentil powders (WL)	650:684	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35840240	8	53	theme	lower	1297:1301	arg1	ingredients					1265:1275	innovative food ingredients	1249:1275	innovative food ingredients higher in fiber but lower in starch compared to ICC	1249:1327	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	8	53	theme	lower	1297:1301	arg1	potential					1236:1244	an important potential	1223:1244	an important potential	1223:1244	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	7	54	dep	showed	1116:1121	arg1	compared					1160:1167	compared	1160:1167	showed a stronger attenuation of amylolysis compared air-dried ICC	1116:1181	Freeze-dried ICC powder showed a stronger attenuation of amylolysis compared air-dried ICC.					
35840240	2	55	theme	conditions	237:246	arg1	effect					213:218	the effect	209:218	the effect of manufacturing conditions on the properties of those powders	209:281	However, the effect of manufacturing conditions on the properties of those powders remained unknown.					
35840240	1	56	theme	Cellular	121:128	arg1	ingredients					167:177	ingredients	167:177	ingredients for different foods	167:197	(Cellular) pulse powders are being proposed as ingredients for different foods.					
35840240	1	56	theme	Cellular	121:128	arg1	powders					137:143	(Cellular) pulse powders	120:143	(Cellular) pulse powders	120:143	(Cellular) pulse powders are being proposed as ingredients for different foods.					
35840240	8	57	theme	innovative	1249:1258	arg1	ingredients					1265:1275	innovative food ingredients	1249:1275	innovative food ingredients higher in fiber but lower in starch compared to ICC	1249:1327	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	8	57	theme	innovative	1249:1258	arg1	potential					1236:1244	an important potential	1223:1244	an important potential	1223:1244	This study showed that WL powders have an important potential as innovative food ingredients higher in fiber but lower in starch compared to ICC.					
35840240	0	58	theme	protein	58:64	arg1	digestibility					66:78	protein digestibility	58:78	protein digestibility	58:78	Effect of manufacturing conditions on in vitro starch and protein digestibility of (cellular) lentil-based ingredients.					
35840240	2	59	theme	manufacturing	223:235	arg1	conditions					237:246	manufacturing conditions	223:246	manufacturing conditions	223:246	However, the effect of manufacturing conditions on the properties of those powders remained unknown.					
35840240	7	60	theme	air-dried	1169:1177	arg1	ICC					1179:1181	air-dried ICC	1169:1181	air-dried ICC	1169:1181	Freeze-dried ICC powder showed a stronger attenuation of amylolysis compared air-dried ICC.					
35840240	4	61	theme	cotyledon	589:597	arg1	ICC					606:608	ICC	606:608	ICC	606:608	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35840240	4	61	theme	cotyledon	589:597	arg1	cells					599:603	isolated cotyledon cells	580:603	isolated cotyledon cells (ICC)	580:609	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35840240	7	62	theme	Freeze-dried	1092:1103	arg1	powder					1109:1114	Freeze-dried ICC powder	1092:1114	Freeze-dried ICC powder	1092:1114	Freeze-dried ICC powder showed a stronger attenuation of amylolysis compared air-dried ICC.					
35840240	0	63	theme	manufacturing	10:22	arg1	conditions					24:33	manufacturing conditions	10:33	manufacturing conditions	10:33	Effect of manufacturing conditions on in vitro starch and protein digestibility of (cellular) lentil-based ingredients.					
35840240	1	64	theme	pulse	131:135	arg1	ingredients					167:177	ingredients	167:177	ingredients for different foods	167:197	(Cellular) pulse powders are being proposed as ingredients for different foods.					
35840240	1	64	theme	pulse	131:135	arg1	powders					137:143	(Cellular) pulse powders	120:143	(Cellular) pulse powders	120:143	(Cellular) pulse powders are being proposed as ingredients for different foods.					
35840240	7	65	theme	amylolysis	1149:1158	arg1	attenuation					1134:1144	a stronger attenuation	1123:1144	a stronger attenuation of amylolysis	1123:1158	Freeze-dried ICC powder showed a stronger attenuation of amylolysis compared air-dried ICC.					
35840240	4	66	theme	isolated	580:587	arg1	ICC					606:608	ICC	606:608	ICC	606:608	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35840240	4	66	theme	isolated	580:587	arg1	cells					599:603	isolated cotyledon cells	580:603	isolated cotyledon cells (ICC)	580:609	Next to powders consisting of isolated cotyledon cells (ICC), this study proposes the production of precooked whole lentil powders (WL), without a cellular isolation step.					
35590319	9	0	theme	horse	1799:1803	arg1	environment					1809:1819	the horse gut environment	1795:1819	the horse gut environment	1795:1819	CONCLUSIONS A high-starch diet causes significant changes in the horse gut environment.					
35590319	4	1	theme	pelleted	1136:1143	arg1	feed					1145:1148	fibre-rich pelleted feed	1125:1148	fibre-rich pelleted feed	1125:1148	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	4	2	theme	pelleted	1044:1051	arg1	feed					1053:1056	pelleted feed	1044:1056	pelleted feed	1044:1056	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	2	3	theme	specific	479:486	arg1	parameters					488:497	specific parameters	479:497	specific parameters of the gut environment across different intestinal compartments of the horse digestive tract	479:590	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	4	4	theme	group	954:958	arg1	pens					960:963	two group pens	950:963	two group pens	950:963	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	4	5	theme	%	1110:1110	arg1	hay					1112:1114	70% hay	1108:1114	70% hay	1108:1114	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	7	6	theme	higher	1508:1513	arg1	proportion					1515:1524	a higher proportion	1506:1524	a higher proportion of particles that washed through the finest sieve (< 1 mm)	1506:1583	In these latter intestinal compartments, horses fed a HS diet also showed a higher proportion of particles retained on an 8 mm sieve and a higher proportion of particles that washed through the finest sieve (< 1 mm).					
35590319	2	7	theme	gut	506:508	arg1	environment					510:520	the gut environment	502:520	the gut environment across different intestinal compartments of the horse digestive tract	502:590	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	9	8	theme	gut	1805:1807	arg1	environment					1809:1819	the horse gut environment	1795:1819	the horse gut environment	1795:1819	CONCLUSIONS A high-starch diet causes significant changes in the horse gut environment.					
35590319	8	9	theme	total	1600:1604	arg1	acid					1657:1660	valeric acid	1649:1660	volatile fatty acids as well as valeric acid	1617:1660	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	8	9	theme	total	1600:1604	arg1	acids					1632:1636	volatile fatty acids	1617:1636	volatile fatty acids as well as valeric acid	1617:1660	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	8	9	theme	total	1600:1604	arg1	higher					1693:1698	higher	1693:1698	higher	1693:1698	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	8	9	theme	total	1600:1604	arg1	amounts					1606:1612	the total amounts	1596:1612	the total amounts of volatile fatty acids as well as valeric acid	1596:1660	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	5	10	theme	dry	1190:1192	arg1	content					1201:1207	a higher dry matter content	1181:1207	a higher dry matter content	1181:1207	Horses fed HS diet presented a higher dry matter content in the right dorsal colon.					
35590319	4	11	dep	starch-rich	1032:1042	arg1	HS					1004:1005	HS	1004:1005	HS; n = 9	1004:1012	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	1	12	theme	high	169:172	arg1	starch					185:190	starch	185:190	starch	185:190	BACKGROUND Horses are often fed high amounts of starch in their diets despite the well-established benefits of a fibre-based diet to promote gut health and animal welfare.					
35590319	1	12	theme	high	169:172	arg1	amounts					174:180	high amounts	169:180	high amounts of starch in their diets	169:205	BACKGROUND Horses are often fed high amounts of starch in their diets despite the well-established benefits of a fibre-based diet to promote gut health and animal welfare.					
35590319	4	13	dep	hay	1112:1114	arg1	feed					1145:1148	fibre-rich pelleted feed	1125:1148	fibre-rich pelleted feed	1125:1148	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	2	14	theme	fibre	463:467	arg1	HF					470:471	HF	470:471	HF	470:471	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	14	theme	fibre	463:467	arg1	fibre					463:467	fibre	463:467	fibre	463:467	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	14	theme	fibre	463:467	arg1	amounts					452:458	high amounts	447:458	high amounts of fibre (HF)	447:472	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	7	15	theme	8 mm	1491:1494	arg1	sieve					1496:1500	an 8 mm sieve	1488:1500	an 8 mm sieve	1488:1500	In these latter intestinal compartments, horses fed a HS diet also showed a higher proportion of particles retained on an 8 mm sieve and a higher proportion of particles that washed through the finest sieve (< 1 mm).					
35590319	4	16	theme	high	980:983	arg1	starch					996:1001	starch	996:1001	starch	996:1001	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	4	16	theme	high	980:983	arg1	amounts					985:991	high amounts	980:991	high amounts of starch	980:1001	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	10	17	from	content	1864:1870	arg1	colon					1892:1896	the right dorsal colon	1875:1896	the right dorsal colon	1875:1896	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	4	18	dep	RESULTS	843:849	arg1	divided					937:943	divided	937:943	were divided into two group pens	932:963	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	3	19	dep	assessed	687:694	arg1	fed					671:673	fed	671:673	fed	671:673	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	6	20	theme	organic	1267:1273	arg1	matter					1275:1280	a higher organic matter	1258:1280	a higher organic matter	1258:1280	Moreover, they showed a higher organic matter and ash content in the sternal flexure, pelvic flexure, right dorsal colon and rectum.					
35590319	10	21	theme	valeric	1970:1976	arg1	acid					1978:1981	valeric acid	1970:1981	valeric acid	1970:1981	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	0	22	theme	horse	114:118	arg1	tract					130:134	the horse digestive tract	110:134	the horse digestive tract	110:134	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.					
35590319	8	23	theme	HS	1718:1719	arg1	diet					1728:1731	the HS vs. HF diet	1714:1731	diet	1728:1731	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	11	24	from	health	2121:2126	arg1	horses					2131:2136	horses	2131:2136	horses	2131:2136	High-starch diets should be avoided in favour of fibre-based diets with the goal of safeguarding gut health in horses.					
35590319	11	25	theme	diets	2081:2085	arg1	favour					2059:2064	favour	2059:2064	favour of fibre-based diets with the goal of safeguarding gut health in horses	2059:2136	High-starch diets should be avoided in favour of fibre-based diets with the goal of safeguarding gut health in horses.					
35590319	0	26	theme	tract	130:134	arg1	compartments					94:105	the different intestinal compartments	69:105	the different intestinal compartments of the horse digestive tract	69:134	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.					
35590319	1	27	from	amounts	174:180	arg1	diets					201:205	their diets	195:205	their diets	195:205	BACKGROUND Horses are often fed high amounts of starch in their diets despite the well-established benefits of a fibre-based diet to promote gut health and animal welfare.					
35590319	3	28	theme	ash	739:741	arg1	content					743:749	ash content	739:749	ash content	739:749	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	6	29	theme	pelvic	1322:1327	arg1	flexure					1313:1319	the sternal flexure	1301:1319	the sternal flexure	1301:1319	Moreover, they showed a higher organic matter and ash content in the sternal flexure, pelvic flexure, right dorsal colon and rectum.					
35590319	6	29	theme	pelvic	1322:1327	arg1	flexure					1329:1335	pelvic flexure	1322:1335	pelvic flexure	1322:1335	Moreover, they showed a higher organic matter and ash content in the sternal flexure, pelvic flexure, right dorsal colon and rectum.					
35590319	3	30	theme	matter	728:733	arg1	terms					699:703	terms	699:703	terms of dry matter, organic matter and ash content	699:749	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	4	31	dep	fibre	1089:1093	arg1	HF					1096:1097	HF	1096:1097	HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed	1096:1148	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	8	32	theme	acid	1657:1660	arg1	acid					1657:1660	valeric acid	1649:1660	volatile fatty acids as well as valeric acid	1617:1660	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	8	32	theme	acid	1657:1660	arg1	acids					1632:1636	volatile fatty acids	1617:1636	volatile fatty acids as well as valeric acid	1617:1660	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	8	32	theme	acid	1657:1660	arg1	higher					1693:1698	higher	1693:1698	higher	1693:1698	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	8	32	theme	acid	1657:1660	arg1	amounts					1606:1612	the total amounts	1596:1612	the total amounts of volatile fatty acids as well as valeric acid	1596:1660	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	4	33	dep	HS	1004:1005	arg1	n = 9					1008:1012	n = 9	1008:1012	HS; n = 9	1004:1012	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	3	34	theme	particle	756:763	arg1	distribution					770:781	the particle size distribution	752:781	the particle size distribution	752:781	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	4	35	theme	14.3 ± 0.7 months	881:897	arg1	horses					871:876	Nineteen Bardigiano horses	851:876	Nineteen Bardigiano horses of 14.3 ± 0.7 months of age	851:904	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	4	36	theme	%	1017:1017	arg1	hay					1019:1021	43% hay plus 57%	1015:1030	hay	1019:1021	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	6	37	dep	dorsal	1344:1349	arg1	rectum					1361:1366	rectum	1361:1366	rectum	1361:1366	Moreover, they showed a higher organic matter and ash content in the sternal flexure, pelvic flexure, right dorsal colon and rectum.					
35590319	6	37	dep	dorsal	1344:1349	arg1	colon					1351:1355	colon	1351:1355	colon	1351:1355	Moreover, they showed a higher organic matter and ash content in the sternal flexure, pelvic flexure, right dorsal colon and rectum.					
35590319	11	38	theme	gut	2117:2119	arg1	health					2121:2126	gut health	2117:2126	gut health in horses	2117:2136	High-starch diets should be avoided in favour of fibre-based diets with the goal of safeguarding gut health in horses.					
35590319	3	39	theme	dry	708:710	arg1	matter					712:717	dry matter	708:717	dry matter	708:717	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	3	40	theme	volatile	787:794	arg1	composition					807:817	volatile fatty acid composition	787:817	volatile fatty acid composition	787:817	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	4	41	dep	HF	1096:1097	arg1	hay					1112:1114	70% hay	1108:1114	70% hay	1108:1114	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	4	41	dep	HF	1096:1097	arg1	%					1123:1123	30%	1121:1123	30%	1121:1123	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	4	41	dep	HF	1096:1097	arg1	n = 10					1100:1105	n = 10	1100:1105	n = 10	1100:1105	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	0	42	from	effects	35:41	arg1	environment					54:64	the gut environment	46:64	the gut environment of the different intestinal compartments of the horse digestive tract	46:134	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.					
35590319	5	43	theme	dorsal	1222:1227	arg1	colon					1229:1233	the right dorsal colon	1212:1233	the right dorsal colon	1212:1233	Horses fed HS diet presented a higher dry matter content in the right dorsal colon.					
35590319	2	44	theme	different	529:537	arg1	compartments					550:561	different intestinal compartments	529:561	different intestinal compartments of the horse digestive tract	529:590	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	10	45	theme	dorsal	1885:1890	arg1	colon					1892:1896	the right dorsal colon	1875:1896	the right dorsal colon	1875:1896	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	0	46	theme	intestinal	83:92	arg1	compartments					94:105	the different intestinal compartments	69:105	the different intestinal compartments of the horse digestive tract	69:134	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.					
35590319	2	47	dep	diets	382:386	arg1	one					390:392	one	390:392	one	390:392	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	47	dep	diets	382:386	arg1	base					439:442	one base	435:442	one base	435:442	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	48	theme	digestive	576:584	arg1	tract					586:590	the horse digestive tract	566:590	the horse digestive tract	566:590	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	4	49	theme	70	1108:1109	arg1	%					1110:1110	%	1110:1110	%	1110:1110	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	1	50	theme	fibre-based	250:260	arg1	diet					262:265	a fibre-based diet to promote gut health and animal welfare	248:306	a fibre-based diet to promote gut health and animal welfare	248:306	BACKGROUND Horses are often fed high amounts of starch in their diets despite the well-established benefits of a fibre-based diet to promote gut health and animal welfare.					
35590319	2	51	theme	different	372:380	arg1	diets					382:386	two different diets	368:386	two different diets - one based on high amounts of starch (HS) vs. one base	368:442	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	3	52	theme	acid	802:805	arg1	composition					807:817	volatile fatty acid composition	787:817	volatile fatty acid composition	787:817	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	4	53	theme	age	902:904	arg1	14.3 ± 0.7 months					881:897	14.3 ± 0.7 months	881:897	14.3 ± 0.7 months of age	881:904	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	1	54	theme	gut	278:280	arg1	health					282:287	gut health	278:287	gut health	278:287	BACKGROUND Horses are often fed high amounts of starch in their diets despite the well-established benefits of a fibre-based diet to promote gut health and animal welfare.					
35590319	2	55	theme	high	403:406	arg1	HS					427:428	HS	427:428	HS	427:428	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	55	theme	high	403:406	arg1	starch					419:424	starch	419:424	starch (HS)	419:429	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	55	theme	high	403:406	arg1	amounts					408:414	high amounts	403:414	high amounts of starch (HS)	403:429	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	10	56	from	increase	1940:1947	arg1	content					1864:1870	the dry matter content	1849:1870	the dry matter content in the right dorsal colon	1849:1896	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	10	56	from	increase	1940:1947	arg1	sizes					1927:1931	reduced particle sizes	1910:1931	reduced particle sizes	1910:1931	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	10	56	from	increase	1940:1947	arg1	production					1956:1965	the production	1952:1965	the production of valeric acid in all the gut compartments studied	1952:2017	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	5	57	theme	HS	1163:1164	arg1	diet					1166:1169	HS diet	1163:1169	HS diet	1163:1169	Horses fed HS diet presented a higher dry matter content in the right dorsal colon.					
35590319	0	58	dep	diet	29:32	arg1	effects					35:41	effects	35:41	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.	0:135	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.					
35590319	9	59	theme	significant	1772:1782	arg1	changes					1784:1790	significant changes	1772:1790	significant changes	1772:1790	CONCLUSIONS A high-starch diet causes significant changes in the horse gut environment.					
35590319	4	60	theme	fibre	1089:1093	arg1	fibre					1089:1093	fibre	1089:1093	fibre	1089:1093	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	4	60	theme	fibre	1089:1093	arg1	amounts					1078:1084	high amounts	1073:1084	high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed)	1073:1149	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	7	61	theme	higher	1445:1450	arg1	proportion					1452:1461	a higher proportion	1443:1461	a higher proportion of particles retained on an 8 mm sieve	1443:1500	In these latter intestinal compartments, horses fed a HS diet also showed a higher proportion of particles retained on an 8 mm sieve and a higher proportion of particles that washed through the finest sieve (< 1 mm).					
35590319	2	62	theme	study	332:336	arg1	aim					313:315	The aim	309:315	The aim of the present study	309:336	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	7	63	theme	particles	1529:1537	arg1	proportion					1452:1461	a higher proportion	1443:1461	a higher proportion of particles retained on an 8 mm sieve	1443:1500	In these latter intestinal compartments, horses fed a HS diet also showed a higher proportion of particles retained on an 8 mm sieve and a higher proportion of particles that washed through the finest sieve (< 1 mm).					
35590319	7	63	theme	particles	1529:1537	arg1	proportion					1515:1524	a higher proportion	1506:1524	a higher proportion of particles that washed through the finest sieve (< 1 mm)	1506:1583	In these latter intestinal compartments, horses fed a HS diet also showed a higher proportion of particles retained on an 8 mm sieve and a higher proportion of particles that washed through the finest sieve (< 1 mm).					
35590319	3	64	theme	end	601:603	arg1	differences					605:615	this end differences	596:615	this end differences in the gastrointestinal environment between HS vs. HF	596:669	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	1	65	theme	well-established	219:234	arg1	benefits					236:243	the well-established benefits	215:243	the well-established benefits of a fibre-based diet to promote gut health and animal welfare	215:306	BACKGROUND Horses are often fed high amounts of starch in their diets despite the well-established benefits of a fibre-based diet to promote gut health and animal welfare.					
35590319	4	66	theme	fibre-rich	1125:1134	arg1	feed					1145:1148	fibre-rich pelleted feed	1125:1148	fibre-rich pelleted feed	1125:1148	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	10	67	theme	matter	1857:1862	arg1	content					1864:1870	the dry matter content	1849:1870	the dry matter content in the right dorsal colon	1849:1896	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	10	68	theme	particle	1918:1925	arg1	sizes					1927:1931	reduced particle sizes	1910:1931	reduced particle sizes	1910:1931	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	3	69	theme	gastrointestinal	624:639	arg1	environment					641:651	the gastrointestinal environment	620:651	the gastrointestinal environment	620:651	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	5	70	theme	right	1216:1220	arg1	colon					1229:1233	the right dorsal colon	1212:1233	the right dorsal colon	1212:1233	Horses fed HS diet presented a higher dry matter content in the right dorsal colon.					
35590319	2	71	theme	environment	510:520	arg1	parameters					488:497	specific parameters	479:497	specific parameters of the gut environment across different intestinal compartments of the horse digestive tract	479:590	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	10	72	from	increase	1837:1844	arg1	content					1864:1870	the dry matter content	1849:1870	the dry matter content in the right dorsal colon	1849:1896	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	10	72	from	increase	1837:1844	arg1	sizes					1927:1931	reduced particle sizes	1910:1931	reduced particle sizes	1910:1931	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	10	72	from	increase	1837:1844	arg1	production					1956:1965	the production	1952:1965	the production of valeric acid in all the gut compartments studied	1952:2017	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	3	73	from	differences	605:615	arg1	environment					641:651	the gastrointestinal environment	620:651	the gastrointestinal environment	620:651	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	5	74	theme	higher	1183:1188	arg1	content					1201:1207	a higher dry matter content	1181:1207	a higher dry matter content	1181:1207	Horses fed HS diet presented a higher dry matter content in the right dorsal colon.					
35590319	6	75	theme	ash	1286:1288	arg1	content					1290:1296	ash content	1286:1296	ash content	1286:1296	Moreover, they showed a higher organic matter and ash content in the sternal flexure, pelvic flexure, right dorsal colon and rectum.					
35590319	7	76	theme	latter	1378:1383	arg1	compartments					1396:1407	these latter intestinal compartments	1372:1407	these latter intestinal compartments	1372:1407	In these latter intestinal compartments, horses fed a HS diet also showed a higher proportion of particles retained on an 8 mm sieve and a higher proportion of particles that washed through the finest sieve (< 1 mm).					
35590319	8	77	theme	volatile	1617:1624	arg1	acids					1632:1636	volatile fatty acids	1617:1636	volatile fatty acids as well as valeric acid	1617:1660	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	5	78	theme	matter	1194:1199	arg1	content					1201:1207	a higher dry matter content	1181:1207	a higher dry matter content	1181:1207	Horses fed HS diet presented a higher dry matter content in the right dorsal colon.					
35590319	10	79	theme	acid	1978:1981	arg1	production					1956:1965	the production	1952:1965	the production of valeric acid in all the gut compartments studied	1952:2017	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	4	80	theme	starch	996:1001	arg1	starch					996:1001	starch	996:1001	starch	996:1001	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	4	80	theme	starch	996:1001	arg1	amounts					985:991	high amounts	980:991	high amounts of starch	980:1001	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	0	81	theme	digestive	120:128	arg1	tract					130:134	the horse digestive tract	110:134	the horse digestive tract	110:134	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.					
35590319	6	82	theme	higher	1260:1265	arg1	matter					1275:1280	a higher organic matter	1258:1280	a higher organic matter	1258:1280	Moreover, they showed a higher organic matter and ash content in the sternal flexure, pelvic flexure, right dorsal colon and rectum.					
35590319	1	83	theme	starch	185:190	arg1	starch					185:190	starch	185:190	starch	185:190	BACKGROUND Horses are often fed high amounts of starch in their diets despite the well-established benefits of a fibre-based diet to promote gut health and animal welfare.					
35590319	1	83	theme	starch	185:190	arg1	amounts					174:180	high amounts	169:180	high amounts of starch in their diets	169:205	BACKGROUND Horses are often fed high amounts of starch in their diets despite the well-established benefits of a fibre-based diet to promote gut health and animal welfare.					
35590319	2	84	theme	high	447:450	arg1	HF					470:471	HF	470:471	HF	470:471	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	84	theme	high	447:450	arg1	fibre					463:467	fibre	463:467	fibre	463:467	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	84	theme	high	447:450	arg1	amounts					452:458	high amounts	447:458	high amounts of fibre (HF)	447:472	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	10	85	theme	dry	1853:1855	arg1	content					1864:1870	the dry matter content	1849:1870	the dry matter content in the right dorsal colon	1849:1896	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	7	86	theme	intestinal	1385:1394	arg1	compartments					1396:1407	these latter intestinal compartments	1372:1407	these latter intestinal compartments	1372:1407	In these latter intestinal compartments, horses fed a HS diet also showed a higher proportion of particles retained on an 8 mm sieve and a higher proportion of particles that washed through the finest sieve (< 1 mm).					
35590319	6	87	theme	right	1338:1342	arg1	flexure					1313:1319	the sternal flexure	1301:1319	the sternal flexure	1301:1319	Moreover, they showed a higher organic matter and ash content in the sternal flexure, pelvic flexure, right dorsal colon and rectum.					
35590319	6	87	theme	right	1338:1342	arg1	dorsal					1344:1349	right dorsal colon and rectum	1338:1366	right dorsal colon and rectum	1338:1366	Moreover, they showed a higher organic matter and ash content in the sternal flexure, pelvic flexure, right dorsal colon and rectum.					
35590319	10	88	theme	gut	1994:1996	arg1	compartments					1998:2009	all the gut compartments	1986:2009	all the gut compartments studied	1986:2017	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	8	89	theme	valeric	1649:1655	arg1	acid					1657:1660	valeric acid	1649:1660	volatile fatty acids as well as valeric acid	1617:1660	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	3	90	theme	content	743:749	arg1	terms					699:703	terms	699:703	terms of dry matter, organic matter and ash content	699:749	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	0	91	theme	gut	50:52	arg1	environment					54:64	the gut environment	46:64	the gut environment of the different intestinal compartments of the horse digestive tract	46:134	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.					
35590319	7	92	theme	particles	1466:1474	arg1	proportion					1452:1461	a higher proportion	1443:1461	a higher proportion of particles retained on an 8 mm sieve	1443:1500	In these latter intestinal compartments, horses fed a HS diet also showed a higher proportion of particles retained on an 8 mm sieve and a higher proportion of particles that washed through the finest sieve (< 1 mm).					
35590319	7	92	theme	particles	1466:1474	arg1	proportion					1515:1524	a higher proportion	1506:1524	a higher proportion of particles that washed through the finest sieve (< 1 mm)	1506:1583	In these latter intestinal compartments, horses fed a HS diet also showed a higher proportion of particles retained on an 8 mm sieve and a higher proportion of particles that washed through the finest sieve (< 1 mm).					
35590319	9	93	dep	CONCLUSIONS	1734:1744	arg1	causes					1765:1770	causes	1765:1770	causes significant changes in the horse gut environment	1765:1819	CONCLUSIONS A high-starch diet causes significant changes in the horse gut environment.					
35590319	4	94	theme	Bardigiano	860:869	arg1	horses					871:876	Nineteen Bardigiano horses	851:876	Nineteen Bardigiano horses of 14.3 ± 0.7 months of age	851:904	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	5	95	attach	presented	1171:1179	arg2	Horses					1152:1157	Horses	1152:1157	Horses fed HS diet	1152:1169	Horses fed HS diet presented a higher dry matter content in the right dorsal colon.					
35590319	5	95	attach	presented	1171:1179	arg1	colon					1229:1233	the right dorsal colon	1212:1233	the right dorsal colon	1212:1233	Horses fed HS diet presented a higher dry matter content in the right dorsal colon.					
35590319	6	96	theme	sternal	1305:1311	arg1	flexure					1313:1319	the sternal flexure	1301:1319	the sternal flexure	1301:1319	Moreover, they showed a higher organic matter and ash content in the sternal flexure, pelvic flexure, right dorsal colon and rectum.					
35590319	6	96	theme	sternal	1305:1311	arg1	flexure					1329:1335	pelvic flexure	1322:1335	pelvic flexure	1322:1335	Moreover, they showed a higher organic matter and ash content in the sternal flexure, pelvic flexure, right dorsal colon and rectum.					
35590319	6	96	theme	sternal	1305:1311	arg1	dorsal					1344:1349	right dorsal colon and rectum	1338:1366	right dorsal colon and rectum	1338:1366	Moreover, they showed a higher organic matter and ash content in the sternal flexure, pelvic flexure, right dorsal colon and rectum.					
35590319	1	97	theme	BACKGROUND	137:146	arg1	Horses					148:153	BACKGROUND Horses	137:153	BACKGROUND Horses	137:153	BACKGROUND Horses are often fed high amounts of starch in their diets despite the well-established benefits of a fibre-based diet to promote gut health and animal welfare.					
35590319	3	98	theme	size	765:768	arg1	distribution					770:781	the particle size distribution	752:781	the particle size distribution	752:781	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	2	99	theme	starch	419:424	arg1	HS					427:428	HS	427:428	HS	427:428	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	99	theme	starch	419:424	arg1	starch					419:424	starch	419:424	starch (HS)	419:429	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	99	theme	starch	419:424	arg1	amounts					408:414	high amounts	403:414	high amounts of starch (HS)	403:429	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	11	100	theme	High-starch	2020:2030	arg1	diets					2032:2036	High-starch diets	2020:2036	High-starch diets	2020:2036	High-starch diets should be avoided in favour of fibre-based diets with the goal of safeguarding gut health in horses.					
35590319	3	101	theme	organic	720:726	arg1	matter					728:733	organic matter	720:733	organic matter	720:733	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	0	102	theme	different	73:81	arg1	compartments					94:105	the different intestinal compartments	69:105	the different intestinal compartments of the horse digestive tract	69:134	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.					
35590319	2	103	theme	intestinal	539:548	arg1	compartments					550:561	different intestinal compartments	529:561	different intestinal compartments of the horse digestive tract	529:590	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	4	104	theme	high	1073:1076	arg1	fibre					1089:1093	fibre	1089:1093	fibre	1089:1093	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	4	104	theme	high	1073:1076	arg1	amounts					1078:1084	high amounts	1073:1084	high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed)	1073:1149	RESULTS Nineteen Bardigiano horses of 14.3 ± 0.7 months of age and destined to slaughter were divided into two group pens - one fed with high amounts of starch (HS; n = 9; 43% hay plus 57% starch-rich pelleted feed); vs. fed with high amounts of fibre (HF; n = 10; 70% hay plus 30% fibre-rich pelleted feed).					
35590319	3	105	theme	matter	712:717	arg1	terms					699:703	terms	699:703	terms of dry matter, organic matter and ash content	699:749	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	0	106	theme	compartments	94:105	arg1	environment					54:64	the gut environment	46:64	the gut environment of the different intestinal compartments of the horse digestive tract	46:134	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.					
35590319	3	107	theme	fatty	796:800	arg1	composition					807:817	volatile fatty acid composition	787:817	volatile fatty acid composition	787:817	To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash content; the particle size distribution and volatile fatty acid composition were also investigated.					
35590319	11	108	with	diets	2081:2085	arg1	goal					2096:2099	the goal	2092:2099	the goal of safeguarding gut health in horses	2092:2136	High-starch diets should be avoided in favour of fibre-based diets with the goal of safeguarding gut health in horses.					
35590319	2	109	theme	tract	586:590	arg1	compartments					550:561	different intestinal compartments	529:561	different intestinal compartments of the horse digestive tract	529:590	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	0	110	theme	high-starch	2:12	arg1	diet					29:32	A high-starch vs. high-fibre diet	0:32	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.	0:135	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.					
35590319	7	111	theme	HS	1423:1424	arg1	diet					1426:1429	a HS diet	1421:1429	a HS diet	1421:1429	In these latter intestinal compartments, horses fed a HS diet also showed a higher proportion of particles retained on an 8 mm sieve and a higher proportion of particles that washed through the finest sieve (< 1 mm).					
35590319	8	112	theme	acids	1632:1636	arg1	acid					1657:1660	valeric acid	1649:1660	volatile fatty acids as well as valeric acid	1617:1660	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	8	112	theme	acids	1632:1636	arg1	acids					1632:1636	volatile fatty acids	1617:1636	volatile fatty acids as well as valeric acid	1617:1660	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	8	112	theme	acids	1632:1636	arg1	higher					1693:1698	higher	1693:1698	higher	1693:1698	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	8	112	theme	acids	1632:1636	arg1	amounts					1606:1612	the total amounts	1596:1612	the total amounts of volatile fatty acids as well as valeric acid	1596:1660	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	10	113	from	sizes	1927:1931	arg1	colon					1892:1896	the right dorsal colon	1875:1896	the right dorsal colon	1875:1896	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	2	114	theme	horse	570:574	arg1	tract					586:590	the horse digestive tract	566:590	the horse digestive tract	566:590	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	0	115	theme	high-fibre	18:27	arg1	diet					29:32	A high-starch vs. high-fibre diet	0:32	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.	0:135	A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.					
35590319	1	116	theme	diet	262:265	arg1	benefits					236:243	the well-established benefits	215:243	the well-established benefits of a fibre-based diet to promote gut health and animal welfare	215:306	BACKGROUND Horses are often fed high amounts of starch in their diets despite the well-established benefits of a fibre-based diet to promote gut health and animal welfare.					
35590319	10	117	theme	right	1879:1883	arg1	colon					1892:1896	the right dorsal colon	1875:1896	the right dorsal colon	1875:1896	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	2	118	theme	diets	382:386	arg1	effects					357:363	the effects	353:363	the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract	353:590	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	8	119	theme	HF	1725:1726	arg1	diet					1728:1731	the HS vs. HF diet	1714:1731	diet	1728:1731	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	9	120	theme	high-starch	1748:1758	arg1	diet					1760:1763	A high-starch diet	1746:1763	A high-starch diet	1746:1763	CONCLUSIONS A high-starch diet causes significant changes in the horse gut environment.					
35590319	8	121	theme	fatty	1626:1630	arg1	acids					1632:1636	volatile fatty acids	1617:1636	volatile fatty acids as well as valeric acid	1617:1660	Moreover, the total amounts of volatile fatty acids as well as valeric acid were found to be significantly higher in horses fed the HS vs. HF diet.					
35590319	10	122	from	production	1956:1965	arg1	compartments					1998:2009	all the gut compartments	1986:2009	all the gut compartments studied	1986:2017	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	1	123	theme	animal	293:298	arg1	welfare					300:306	animal welfare	293:306	animal welfare	293:306	BACKGROUND Horses are often fed high amounts of starch in their diets despite the well-established benefits of a fibre-based diet to promote gut health and animal welfare.					
35590319	10	124	theme	reduced	1910:1916	arg1	sizes					1927:1931	reduced particle sizes	1910:1931	reduced particle sizes	1910:1931	We observed an increase in the dry matter content in the right dorsal colon, as well as reduced particle sizes and an increase in the production of valeric acid in all the gut compartments studied.					
35590319	7	125	theme	finest	1563:1568	arg1	< 1 mm					1577:1582	< 1 mm	1577:1582	< 1 mm	1577:1582	In these latter intestinal compartments, horses fed a HS diet also showed a higher proportion of particles retained on an 8 mm sieve and a higher proportion of particles that washed through the finest sieve (< 1 mm).					
35590319	7	125	theme	finest	1563:1568	arg1	sieve					1570:1574	the finest sieve	1559:1574	the finest sieve (< 1 mm)	1559:1583	In these latter intestinal compartments, horses fed a HS diet also showed a higher proportion of particles retained on an 8 mm sieve and a higher proportion of particles that washed through the finest sieve (< 1 mm).					
35590319	11	126	theme	fibre-based	2069:2079	arg1	diets					2081:2085	fibre-based diets	2069:2085	fibre-based diets with the goal of safeguarding gut health in horses	2069:2136	High-starch diets should be avoided in favour of fibre-based diets with the goal of safeguarding gut health in horses.					
35590319	2	127	from	effects	357:363	arg1	parameters					488:497	specific parameters	479:497	specific parameters of the gut environment across different intestinal compartments of the horse digestive tract	479:590	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	127	from	effects	357:363	arg1	HF					470:471	HF	470:471	HF	470:471	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	127	from	effects	357:363	arg1	fibre					463:467	fibre	463:467	fibre	463:467	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	127	from	effects	357:363	arg1	amounts					452:458	high amounts	447:458	high amounts of fibre (HF)	447:472	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35590319	2	128	theme	present	324:330	arg1	study					332:336	the present study	320:336	the present study	320:336	The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract.					
35075560	6	0	theme	adsorption	889:898	arg1	mechanisms					900:909	the adsorption mechanisms	885:909	the adsorption mechanisms involved in the process	885:933	For elucidating the adsorption mechanisms involved in the process, the non-linear forms of kinetic and isotherm models were used.					
35075560	2	1	theme	coconut	368:374	arg1	fibers					376:381	crude and modified coconut fibers	349:381	crude and modified coconut fibers from aqueous systems	349:402	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	4	2	theme	FTIR	611:614	arg1	analyses					713:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	0	3	theme	coir-based	74:83	arg1	biosorbents					85:95	mesoporous coconut coir-based biosorbents	55:95	mesoporous coconut coir-based biosorbents	55:95	Elucidating the adsorption mechanism of Rhodamine B on mesoporous coconut coir-based biosorbents through a non-linear modeling and recycling approach.					
35075560	11	4	theme	traditional	1580:1590	arg1	materials					1602:1610	the traditional adsorbent materials	1576:1610	the traditional adsorbent materials	1576:1610	Therefore, the coconut fiber can be considered an alternative to the traditional adsorbent materials that allows the reuse by four times without performance loss, in which its adsorptive capacity has increased through its chemical modification by a biorefinery process.					
35075560	1	5	theme	renewable	166:174	arg1	materials					186:194	renewable adsorbent materials	166:194	renewable adsorbent materials	166:194	The search for renewable adsorbent materials has increased continuously, being the agro-wastes an interesting alternative.					
35075560	10	6	theme	higher	1435:1440	arg1	spontaneity					1442:1452	higher spontaneity	1435:1452	higher spontaneity	1435:1452	The dye adsorption was spontaneous and endothermic for both biosorbents, showing higher spontaneity and affinity with the adsorbate for biosorbent modified.					
35075560	11	7	theme	chemical	1733:1740	arg1	modification					1742:1753	its chemical modification	1729:1753	its chemical modification	1729:1753	Therefore, the coconut fiber can be considered an alternative to the traditional adsorbent materials that allows the reuse by four times without performance loss, in which its adsorptive capacity has increased through its chemical modification by a biorefinery process.					
35075560	5	8	from	effects	764:770	arg1	adsorption					857:866	Rhodamine B adsorption	845:866	Rhodamine B adsorption	845:866	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	11	9	theme	adsorbent	1592:1600	arg1	materials					1602:1610	the traditional adsorbent materials	1576:1610	the traditional adsorbent materials	1576:1610	Therefore, the coconut fiber can be considered an alternative to the traditional adsorbent materials that allows the reuse by four times without performance loss, in which its adsorptive capacity has increased through its chemical modification by a biorefinery process.					
35075560	8	10	contain	had	1116:1118	arg1	fiber					1110:1114	Modified coconut fiber	1093:1114	Modified coconut fiber	1093:1114	Modified coconut fiber had an increase in the amount of α-cellulose, which influenced its structural, morphological, surface, and porous properties.					
35075560	8	10	contain	had	1116:1118	arg2	increase					1123:1130	an increase	1120:1130	an increase	1120:1130	Modified coconut fiber had an increase in the amount of α-cellulose, which influenced its structural, morphological, surface, and porous properties.					
35075560	4	11	theme	TGA	617:619	arg1	analyses					713:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	9	12	theme	B	1278:1278	arg1	%					1292:1292	about 90%	1284:1292	about 90% for modified coconut fiber	1284:1319	The removal efficiency of Rhodamine B was about 90% for modified coconut fiber and 36% for crude coconut fiber.					
35075560	9	12	theme	B	1278:1278	arg1	efficiency					1254:1263	The removal efficiency	1242:1263	The removal efficiency of Rhodamine B	1242:1278	The removal efficiency of Rhodamine B was about 90% for modified coconut fiber and 36% for crude coconut fiber.					
35075560	4	13	theme	point	689:693	arg1	TGA					617:619	TGA	617:619	TGA	617:619	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	4	13	theme	point	689:693	arg1	pHPZC					706:710	pHPZC	706:710	pHPZC	706:710	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	4	13	theme	point	689:693	arg1	adsorption/desorption					641:661	nitrogen adsorption/desorption	632:661	nitrogen adsorption/desorption (BET-BJH)	632:671	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	4	13	theme	point	689:693	arg1	pH					678:679	pH	678:679	pH of zero point of charge (pHPZC)	678:711	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	4	13	theme	point	689:693	arg1	composition					598:608	lignocellulosic composition	582:608	lignocellulosic composition	582:608	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	4	13	theme	point	689:693	arg1	WCA					622:624	WCA	622:624	WCA	622:624	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	4	13	theme	point	689:693	arg1	BET-BJH					664:670	BET-BJH	664:670	BET-BJH	664:670	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	4	13	theme	point	689:693	arg1	SEM					627:629	SEM	627:629	SEM	627:629	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	4	13	theme	point	689:693	arg1	FTIR					611:614	FTIR	611:614	FTIR	611:614	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	11	14	dep	which	1677:1681	arg1	in					1674:1675	in	1674:1675	in	1674:1675	Therefore, the coconut fiber can be considered an alternative to the traditional adsorbent materials that allows the reuse by four times without performance loss, in which its adsorptive capacity has increased through its chemical modification by a biorefinery process.					
35075560	9	15	theme	coconut	1339:1345	arg1	fiber					1347:1351	crude coconut fiber	1333:1351	crude coconut fiber	1333:1351	The removal efficiency of Rhodamine B was about 90% for modified coconut fiber and 36% for crude coconut fiber.					
35075560	5	16	theme	contact	812:818	arg1	time					820:823	contact time	812:823	contact time	812:823	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	5	16	theme	contact	812:818	arg1	dosages					803:809	the adsorbent and adsorbate dosages	775:809	the adsorbent and adsorbate dosages	775:809	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	1	17	theme	adsorbent	176:184	arg1	materials					186:194	renewable adsorbent materials	166:194	renewable adsorbent materials	166:194	The search for renewable adsorbent materials has increased continuously, being the agro-wastes an interesting alternative.					
35075560	0	18	theme	non-linear	107:116	arg1	modeling					118:125	a non-linear modeling	105:125	a non-linear modeling	105:125	Elucidating the adsorption mechanism of Rhodamine B on mesoporous coconut coir-based biosorbents through a non-linear modeling and recycling approach.					
35075560	9	19	theme	Rhodamine	1268:1276	arg1	B					1278:1278	Rhodamine B	1268:1278	Rhodamine B	1268:1278	The removal efficiency of Rhodamine B was about 90% for modified coconut fiber and 36% for crude coconut fiber.					
35075560	6	20	theme	isotherm	972:979	arg1	models					981:986	kinetic and isotherm models	960:986	kinetic and isotherm models	960:986	For elucidating the adsorption mechanisms involved in the process, the non-linear forms of kinetic and isotherm models were used.					
35075560	3	21	theme	chemical	456:463	arg1	modification					465:476	The chemical modification	452:476	The chemical modification of crude coconut fiber	452:499	The chemical modification of crude coconut fiber was carried out by the organosolv process.					
35075560	5	22	theme	adsorbent	779:787	arg1	time					820:823	contact time	812:823	contact time	812:823	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	5	22	theme	adsorbent	779:787	arg1	temperature					830:840	temperature	830:840	temperature	830:840	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	5	22	theme	adsorbent	779:787	arg1	dosages					803:809	the adsorbent and adsorbate dosages	775:809	the adsorbent and adsorbate dosages	775:809	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	9	23	theme	coconut	1307:1313	arg1	fiber					1315:1319	modified coconut fiber	1298:1319	modified coconut fiber	1298:1319	The removal efficiency of Rhodamine B was about 90% for modified coconut fiber and 36% for crude coconut fiber.					
35075560	6	24	used	used	993:996	arg2	forms					951:955	the non-linear forms	936:955	the non-linear forms of kinetic and isotherm models	936:986	For elucidating the adsorption mechanisms involved in the process, the non-linear forms of kinetic and isotherm models were used.					
35075560	5	25	theme	batch	727:731	arg1	tests					744:748	The batch adsorption tests	723:748	The batch adsorption tests	723:748	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	2	26	theme	Rhodamine	334:342	arg1	B					344:344	Rhodamine B	334:344	Rhodamine B	334:344	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	8	27	theme	α-cellulose	1149:1159	arg1	α-cellulose					1149:1159	α-cellulose	1149:1159	α-cellulose	1149:1159	Modified coconut fiber had an increase in the amount of α-cellulose, which influenced its structural, morphological, surface, and porous properties.					
35075560	8	27	theme	α-cellulose	1149:1159	arg1	amount					1139:1144	the amount	1135:1144	the amount of α-cellulose	1135:1159	Modified coconut fiber had an increase in the amount of α-cellulose, which influenced its structural, morphological, surface, and porous properties.					
35075560	8	28	theme	structural	1183:1192	arg1	properties					1230:1239	its structural, morphological, surface, and porous properties	1179:1239	its structural, morphological, surface, and porous properties	1179:1239	Modified coconut fiber had an increase in the amount of α-cellulose, which influenced its structural, morphological, surface, and porous properties.					
35075560	11	29	theme	coconut	1526:1532	arg1	fiber					1534:1538	the coconut fiber	1522:1538	the coconut fiber	1522:1538	Therefore, the coconut fiber can be considered an alternative to the traditional adsorbent materials that allows the reuse by four times without performance loss, in which its adsorptive capacity has increased through its chemical modification by a biorefinery process.					
35075560	8	30	theme	porous	1223:1228	arg1	properties					1230:1239	its structural, morphological, surface, and porous properties	1179:1239	its structural, morphological, surface, and porous properties	1179:1239	Modified coconut fiber had an increase in the amount of α-cellulose, which influenced its structural, morphological, surface, and porous properties.					
35075560	2	31	theme	aqueous	388:394	arg1	systems					396:402	aqueous systems	388:402	aqueous systems	388:402	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	5	32	theme	adsorbate	793:801	arg1	time					820:823	contact time	812:823	contact time	812:823	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	5	32	theme	adsorbate	793:801	arg1	temperature					830:840	temperature	830:840	temperature	830:840	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	5	32	theme	adsorbate	793:801	arg1	dosages					803:809	the adsorbent and adsorbate dosages	775:809	the adsorbent and adsorbate dosages	775:809	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	6	33	theme	non-linear	940:949	arg1	forms					951:955	the non-linear forms	936:955	the non-linear forms of kinetic and isotherm models	936:986	For elucidating the adsorption mechanisms involved in the process, the non-linear forms of kinetic and isotherm models were used.					
35075560	0	34	theme	recycling	131:139	arg1	approach					141:148	recycling approach	131:148	recycling approach	131:148	Elucidating the adsorption mechanism of Rhodamine B on mesoporous coconut coir-based biosorbents through a non-linear modeling and recycling approach.					
35075560	2	35	from	fibers	376:381	arg1	mechanism					307:315	the mechanism	303:315	the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems	303:402	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	2	35	from	fibers	376:381	arg1	feasibility					412:422	the feasibility	408:422	the feasibility of reusing the biosorbents	408:449	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	2	36	theme	adsorption	320:329	arg1	mechanism					307:315	the mechanism	303:315	the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems	303:402	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	2	36	theme	adsorption	320:329	arg1	feasibility					412:422	the feasibility	408:422	the feasibility of reusing the biosorbents	408:449	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	7	37	theme	desorption	1069:1078	arg1	experiments					1080:1090	the desorption experiments	1065:1090	the desorption experiments	1065:1090	The regeneration of the biosorbents was evaluated by carrying out the desorption experiments.					
35075560	3	38	theme	crude	481:485	arg1	fiber					495:499	crude coconut fiber	481:499	crude coconut fiber	481:499	The chemical modification of crude coconut fiber was carried out by the organosolv process.					
35075560	0	39	theme	adsorption	16:25	arg1	mechanism					27:35	the adsorption mechanism	12:35	the adsorption mechanism of Rhodamine B on mesoporous coconut coir-based biosorbents	12:95	Elucidating the adsorption mechanism of Rhodamine B on mesoporous coconut coir-based biosorbents through a non-linear modeling and recycling approach.					
35075560	4	40	theme	composition	598:608	arg1	analyses					713:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	8	41	from	increase	1123:1130	arg1	α-cellulose					1149:1159	α-cellulose	1149:1159	α-cellulose	1149:1159	Modified coconut fiber had an increase in the amount of α-cellulose, which influenced its structural, morphological, surface, and porous properties.					
35075560	8	41	from	increase	1123:1130	arg1	amount					1139:1144	the amount	1135:1144	the amount of α-cellulose	1135:1159	Modified coconut fiber had an increase in the amount of α-cellulose, which influenced its structural, morphological, surface, and porous properties.					
35075560	2	42	theme	modified	359:366	arg1	fibers					376:381	crude and modified coconut fibers	349:381	crude and modified coconut fibers from aqueous systems	349:402	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	3	43	theme	coconut	487:493	arg1	fiber					495:499	crude coconut fiber	481:499	crude coconut fiber	481:499	The chemical modification of crude coconut fiber was carried out by the organosolv process.					
35075560	0	44	theme	Rhodamine	40:48	arg1	B					50:50	Rhodamine B	40:50	Rhodamine B	40:50	Elucidating the adsorption mechanism of Rhodamine B on mesoporous coconut coir-based biosorbents through a non-linear modeling and recycling approach.					
35075560	4	45	theme	lignocellulosic	582:596	arg1	composition					598:608	lignocellulosic composition	582:608	lignocellulosic composition	582:608	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	7	46	theme	biosorbents	1023:1033	arg1	regeneration					1003:1014	The regeneration	999:1014	The regeneration of the biosorbents	999:1033	The regeneration of the biosorbents was evaluated by carrying out the desorption experiments.					
35075560	11	47	theme	performance	1656:1666	arg1	loss					1668:1671	performance loss	1656:1671	performance loss	1656:1671	Therefore, the coconut fiber can be considered an alternative to the traditional adsorbent materials that allows the reuse by four times without performance loss, in which its adsorptive capacity has increased through its chemical modification by a biorefinery process.					
35075560	5	48	theme	Rhodamine	845:853	arg1	adsorption					857:866	Rhodamine B adsorption	845:866	Rhodamine B adsorption	845:866	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	9	49	theme	modified	1298:1305	arg1	fiber					1315:1319	modified coconut fiber	1298:1319	modified coconut fiber	1298:1319	The removal efficiency of Rhodamine B was about 90% for modified coconut fiber and 36% for crude coconut fiber.					
35075560	8	50	theme	surface	1210:1216	arg1	properties					1230:1239	its structural, morphological, surface, and porous properties	1179:1239	its structural, morphological, surface, and porous properties	1179:1239	Modified coconut fiber had an increase in the amount of α-cellulose, which influenced its structural, morphological, surface, and porous properties.					
35075560	1	51	dep	agro-wastes	234:244	arg1	alternative					261:271	an interesting alternative	246:271	the agro-wastes an interesting alternative	230:271	The search for renewable adsorbent materials has increased continuously, being the agro-wastes an interesting alternative.					
35075560	4	52	theme	SEM	627:629	arg1	analyses					713:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	2	53	from	systems	396:402	arg1	fibers					376:381	crude and modified coconut fibers	349:381	crude and modified coconut fibers from aqueous systems	349:402	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	4	54	theme	adsorption/desorption	641:661	arg1	analyses					713:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	8	55	theme	morphological	1195:1207	arg1	properties					1230:1239	its structural, morphological, surface, and porous properties	1179:1239	its structural, morphological, surface, and porous properties	1179:1239	Modified coconut fiber had an increase in the amount of α-cellulose, which influenced its structural, morphological, surface, and porous properties.					
35075560	5	56	theme	B	855:855	arg1	adsorption					857:866	Rhodamine B adsorption	845:866	Rhodamine B adsorption	845:866	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	5	57	theme	adsorption	733:742	arg1	tests					744:748	The batch adsorption tests	723:748	The batch adsorption tests	723:748	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	10	58	theme	dye	1358:1360	arg1	spontaneous					1377:1387	spontaneous	1377:1387	spontaneous	1377:1387	The dye adsorption was spontaneous and endothermic for both biosorbents, showing higher spontaneity and affinity with the adsorbate for biosorbent modified.					
35075560	10	58	theme	dye	1358:1360	arg1	adsorption					1362:1371	The dye adsorption	1354:1371	The dye adsorption	1354:1371	The dye adsorption was spontaneous and endothermic for both biosorbents, showing higher spontaneity and affinity with the adsorbate for biosorbent modified.					
35075560	4	59	theme	charge	698:703	arg1	point					689:693	zero point	684:693	zero point of charge	684:703	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	0	60	theme	B	50:50	arg1	mechanism					27:35	the adsorption mechanism	12:35	the adsorption mechanism of Rhodamine B on mesoporous coconut coir-based biosorbents	12:95	Elucidating the adsorption mechanism of Rhodamine B on mesoporous coconut coir-based biosorbents through a non-linear modeling and recycling approach.					
35075560	11	61	dep	reuse	1628:1632	arg1	increased					1711:1719	increased	1711:1719	has increased through its chemical modification by a biorefinery process	1707:1778	Therefore, the coconut fiber can be considered an alternative to the traditional adsorbent materials that allows the reuse by four times without performance loss, in which its adsorptive capacity has increased through its chemical modification by a biorefinery process.					
35075560	9	62	theme	removal	1246:1252	arg1	%					1292:1292	about 90%	1284:1292	about 90% for modified coconut fiber	1284:1319	The removal efficiency of Rhodamine B was about 90% for modified coconut fiber and 36% for crude coconut fiber.					
35075560	9	62	theme	removal	1246:1252	arg1	efficiency					1254:1263	The removal efficiency	1242:1263	The removal efficiency of Rhodamine B	1242:1278	The removal efficiency of Rhodamine B was about 90% for modified coconut fiber and 36% for crude coconut fiber.					
35075560	6	63	theme	kinetic	960:966	arg1	models					981:986	kinetic and isotherm models	960:986	kinetic and isotherm models	960:986	For elucidating the adsorption mechanisms involved in the process, the non-linear forms of kinetic and isotherm models were used.					
35075560	2	64	theme	crude	349:353	arg1	fibers					376:381	crude and modified coconut fibers	349:381	crude and modified coconut fibers from aqueous systems	349:402	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	8	65	theme	coconut	1102:1108	arg1	fiber					1110:1114	Modified coconut fiber	1093:1114	Modified coconut fiber	1093:1114	Modified coconut fiber had an increase in the amount of α-cellulose, which influenced its structural, morphological, surface, and porous properties.					
35075560	3	66	theme	fiber	495:499	arg1	modification					465:476	The chemical modification	452:476	The chemical modification of crude coconut fiber	452:499	The chemical modification of crude coconut fiber was carried out by the organosolv process.					
35075560	4	67	theme	WCA	622:624	arg1	analyses					713:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	8	68	theme	Modified	1093:1100	arg1	fiber					1110:1114	Modified coconut fiber	1093:1114	Modified coconut fiber	1093:1114	Modified coconut fiber had an increase in the amount of α-cellulose, which influenced its structural, morphological, surface, and porous properties.					
35075560	5	69	theme	dosages	803:809	arg1	effects					764:770	the effects	760:770	the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption	760:866	The batch adsorption tests evaluated the effects of the adsorbent and adsorbate dosages, contact time, and temperature on Rhodamine B adsorption.					
35075560	2	70	theme	B	344:344	arg1	adsorption					320:329	adsorption	320:329	adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems	320:402	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	2	71	from	mechanism	307:315	arg1	fibers					376:381	crude and modified coconut fibers	349:381	crude and modified coconut fibers from aqueous systems	349:402	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	9	72	theme	crude	1333:1337	arg1	fiber					1347:1351	crude coconut fiber	1333:1351	crude coconut fiber	1333:1351	The removal efficiency of Rhodamine B was about 90% for modified coconut fiber and 36% for crude coconut fiber.					
35075560	0	73	theme	coconut	66:72	arg1	biosorbents					85:95	mesoporous coconut coir-based biosorbents	55:95	mesoporous coconut coir-based biosorbents	55:95	Elucidating the adsorption mechanism of Rhodamine B on mesoporous coconut coir-based biosorbents through a non-linear modeling and recycling approach.					
35075560	11	74	theme	adsorptive	1687:1696	arg1	capacity					1698:1705	its adsorptive capacity	1683:1705	its adsorptive capacity	1683:1705	Therefore, the coconut fiber can be considered an alternative to the traditional adsorbent materials that allows the reuse by four times without performance loss, in which its adsorptive capacity has increased through its chemical modification by a biorefinery process.					
35075560	2	75	from	feasibility	412:422	arg1	fibers					376:381	crude and modified coconut fibers	349:381	crude and modified coconut fibers from aqueous systems	349:402	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	3	76	theme	organosolv	524:533	arg1	process					535:541	the organosolv process	520:541	the organosolv process	520:541	The chemical modification of crude coconut fiber was carried out by the organosolv process.					
35075560	11	77	theme	biorefinery	1760:1770	arg1	process					1772:1778	a biorefinery process	1758:1778	a biorefinery process	1758:1778	Therefore, the coconut fiber can be considered an alternative to the traditional adsorbent materials that allows the reuse by four times without performance loss, in which its adsorptive capacity has increased through its chemical modification by a biorefinery process.					
35075560	1	78	theme	interesting	249:259	arg1	alternative					261:271	an interesting alternative	246:271	the agro-wastes an interesting alternative	230:271	The search for renewable adsorbent materials has increased continuously, being the agro-wastes an interesting alternative.					
35075560	0	79	theme	mesoporous	55:64	arg1	biosorbents					85:95	mesoporous coconut coir-based biosorbents	55:95	mesoporous coconut coir-based biosorbents	55:95	Elucidating the adsorption mechanism of Rhodamine B on mesoporous coconut coir-based biosorbents through a non-linear modeling and recycling approach.					
35075560	0	80	from	mechanism	27:35	arg1	biosorbents					85:95	mesoporous coconut coir-based biosorbents	55:95	mesoporous coconut coir-based biosorbents	55:95	Elucidating the adsorption mechanism of Rhodamine B on mesoporous coconut coir-based biosorbents through a non-linear modeling and recycling approach.					
35075560	4	81	theme	nitrogen	632:639	arg1	adsorption/desorption					641:661	nitrogen adsorption/desorption	632:661	nitrogen adsorption/desorption (BET-BJH)	632:671	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	4	81	theme	nitrogen	632:639	arg1	BET-BJH					664:670	BET-BJH	664:670	BET-BJH	664:670	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	4	82	theme	pH	678:679	arg1	analyses					713:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses	582:720	The biosorbents were characterized by lignocellulosic composition, FTIR, TGA, WCA, SEM, nitrogen adsorption/desorption (BET-BJH), and pH of zero point of charge (pHPZC) analyses.					
35075560	2	83	from	adsorption	320:329	arg1	fibers					376:381	crude and modified coconut fibers	349:381	crude and modified coconut fibers from aqueous systems	349:402	This work aimed to elucidate the mechanism of adsorption of Rhodamine B on crude and modified coconut fibers from aqueous systems and the feasibility of reusing the biosorbents.					
35075560	6	84	theme	models	981:986	arg1	forms					951:955	the non-linear forms	936:955	the non-linear forms of kinetic and isotherm models	936:986	For elucidating the adsorption mechanisms involved in the process, the non-linear forms of kinetic and isotherm models were used.					
36262059	6	0	from	increase	990:997	arg1	amount					1006:1011	the amount	1002:1011	the amount of perlecan	1002:1023	There was a significant increase in the amount of perlecan in the prostate stroma from groups subjected to castration plus flutamide for 7 or 30 days.					
36262059	6	0	from	increase	990:997	arg1	stroma					1041:1046	the prostate stroma	1028:1046	the prostate stroma	1028:1046	There was a significant increase in the amount of perlecan in the prostate stroma from groups subjected to castration plus flutamide for 7 or 30 days.					
36262059	6	0	from	increase	990:997	arg1	perlecan					1016:1023	perlecan	1016:1023	perlecan	1016:1023	There was a significant increase in the amount of perlecan in the prostate stroma from groups subjected to castration plus flutamide for 7 or 30 days.					
36262059	3	1	theme	electron	749:756	arg1	microscopy					758:767	transmission electron microscopy	736:767	transmission electron microscopy	736:767	Tissue analysis included immunohistochemical assessment (perlecan and chondroitin sulfate) and proteoglycan morphology was analyzed by transmission electron microscopy.					
36262059	9	2	theme	days	1371:1374	arg1	postcastration					1376:1389	30 days postcastration	1368:1389	30 days postcastration	1368:1389	At 30 days postcastration, with or without flutamide treatment, larger proteoglycans were common.					
36262059	1	3	theme	surgical	269:276	arg1	castration					278:287	surgical castration	269:287	surgical castration	269:287	BACKGROUND The aim of this study was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade.					
36262059	2	4	theme	postsurgery	488:498	arg1	period					500:505	the total postsurgery period	478:505	the total postsurgery period	478:505	METHODS The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals.					
36262059	10	5	dep	frequency	1651:1659	arg1	the					1642:1644	the	1642:1644	the	1642:1644	CONCLUSIONS In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent.					
36262059	1	6	from	modifications	158:170	arg1	composition					203:213	composition	203:213	composition	203:213	BACKGROUND The aim of this study was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade.					
36262059	1	6	from	modifications	158:170	arg1	morphology					188:197	proteoglycan morphology	175:197	proteoglycan morphology	175:197	BACKGROUND The aim of this study was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade.					
36262059	1	7	dep	BACKGROUND	109:118	arg1	was					142:144	was	142:144	was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade	142:338	BACKGROUND The aim of this study was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade.					
36262059	10	8	theme	androgenic	1612:1621	arg1	deprivation					1623:1633	androgenic deprivation	1612:1633	androgenic deprivation	1612:1633	CONCLUSIONS In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent.					
36262059	7	9	theme	areas	1171:1175	arg1	incidence					1158:1166	the incidence	1154:1166	the incidence of areas occupied by proteoglycans and basement membrane	1154:1223	Ultrastructural analysis showed that the incidence of areas occupied by proteoglycans and basement membrane was altered by treatment.					
36262059	7	10	theme	basement	1207:1214	arg1	membrane					1216:1223	basement membrane	1207:1223	basement membrane	1207:1223	Ultrastructural analysis showed that the incidence of areas occupied by proteoglycans and basement membrane was altered by treatment.					
36262059	4	11	theme	Chondroitin	778:788	arg1	frequency					798:806	Chondroitin sulfate frequency	778:806	Chondroitin sulfate frequency	778:806	RESULTS Chondroitin sulfate frequency was increased 7 days postcastration with an androgenic blockade.					
36262059	3	12	dep	assessment	646:655	arg1	sulfate					683:689	chondroitin sulfate	671:689	chondroitin sulfate	671:689	Tissue analysis included immunohistochemical assessment (perlecan and chondroitin sulfate) and proteoglycan morphology was analyzed by transmission electron microscopy.					
36262059	3	12	dep	assessment	646:655	arg1	perlecan					658:665	perlecan	658:665	perlecan	658:665	Tissue analysis included immunohistochemical assessment (perlecan and chondroitin sulfate) and proteoglycan morphology was analyzed by transmission electron microscopy.					
36262059	11	13	from	events	1885:1890	arg1	microenvironment					1908:1923	the prostate microenvironment	1895:1923	the prostate microenvironment	1895:1923	Length and form alterations in proteoglycans as well as associations among them and with the basement membrane were dynamic events in the prostate microenvironment.					
36262059	1	14	theme	proteoglycan	175:186	arg1	morphology					188:197	proteoglycan morphology	175:197	proteoglycan morphology	175:197	BACKGROUND The aim of this study was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade.					
36262059	2	15	theme	total	482:486	arg1	period					500:505	the total postsurgery period	478:505	the total postsurgery period	478:505	METHODS The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals.					
36262059	4	16	theme	postcastration	829:842	arg1	days					824:827	7 days postcastration	822:842	7 days postcastration	822:842	RESULTS Chondroitin sulfate frequency was increased 7 days postcastration with an androgenic blockade.					
36262059	10	17	theme	high	1646:1649	arg1	frequency					1651:1659	high frequency	1646:1659	high frequency	1646:1659	CONCLUSIONS In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent.					
36262059	0	18	theme	old	96:98	arg1	gerbils					100:106	old gerbils	96:106	old gerbils	96:106	Proteoglycans orchestrate remodeling of prostatic cytoarchitecture after androgenic blockade in old gerbils.					
36262059	9	19	from	postcastration	1376:1389	arg1	common					1455:1460	common	1455:1460	common	1455:1460	At 30 days postcastration, with or without flutamide treatment, larger proteoglycans were common.					
36262059	8	20	dep	amount	1310:1315	arg1	the					1306:1308	the	1306:1308	the	1306:1308	In addition, androgenic blockade results in changes in the amount, thickness, and morphology of these structures.					
36262059	6	21	theme	prostate	1032:1039	arg1	stroma					1041:1046	the prostate stroma	1028:1046	the prostate stroma	1028:1046	There was a significant increase in the amount of perlecan in the prostate stroma from groups subjected to castration plus flutamide for 7 or 30 days.					
36262059	2	22	dep	METHODS	341:347	arg1	consisted					572:580	consisted	572:580	consisted of intact animals	572:598	METHODS The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals.					
36262059	2	22	dep	METHODS	341:347	arg1	sorted					374:379	sorted	374:379	were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration	369:551	METHODS The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals.					
36262059	5	23	theme	androgenic	935:944	arg1	treatment					955:963	androgenic blockade treatment	935:963	androgenic blockade treatment	935:963	The presence of these carbohydrates was rare after 30 days of androgenic blockade treatment.					
36262059	2	24	theme	flutamide	437:445	arg1	10 mg/kg/day					447:458	flutamide 10 mg/kg/day	437:458	flutamide 10 mg/kg/day	437:458	METHODS The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals.					
36262059	2	24	theme	flutamide	437:445	arg1	treatment					426:434	antiandrogen treatment	413:434	antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period	413:505	METHODS The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals.					
36262059	8	25	theme	structures	1353:1362	arg1	structures					1353:1362	these structures	1347:1362	these structures	1347:1362	In addition, androgenic blockade results in changes in the amount, thickness, and morphology of these structures.					
36262059	8	25	theme	structures	1353:1362	arg1	thickness					1318:1326	thickness	1318:1326	thickness	1318:1326	In addition, androgenic blockade results in changes in the amount, thickness, and morphology of these structures.					
36262059	8	25	theme	structures	1353:1362	arg1	morphology					1333:1342	morphology	1333:1342	morphology	1333:1342	In addition, androgenic blockade results in changes in the amount, thickness, and morphology of these structures.					
36262059	8	25	theme	structures	1353:1362	arg1	amount					1310:1315	amount	1310:1315	amount	1310:1315	In addition, androgenic blockade results in changes in the amount, thickness, and morphology of these structures.					
36262059	7	26	theme	Ultrastructural	1117:1131	arg1	analysis					1133:1140	Ultrastructural analysis	1117:1140	Ultrastructural analysis	1117:1140	Ultrastructural analysis showed that the incidence of areas occupied by proteoglycans and basement membrane was altered by treatment.					
36262059	5	27	theme	carbohydrates	895:907	arg1	rare					913:916	rare	913:916	rare	913:916	The presence of these carbohydrates was rare after 30 days of androgenic blockade treatment.					
36262059	5	27	theme	carbohydrates	895:907	arg1	presence					877:884	The presence	873:884	The presence of these carbohydrates	873:907	The presence of these carbohydrates was rare after 30 days of androgenic blockade treatment.					
36262059	4	28	theme	sulfate	790:796	arg1	frequency					798:806	Chondroitin sulfate frequency	778:806	Chondroitin sulfate frequency	778:806	RESULTS Chondroitin sulfate frequency was increased 7 days postcastration with an androgenic blockade.					
36262059	2	29	theme	control	558:564	arg1	group					566:570	the control group	554:570	the control group	554:570	METHODS The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals.					
36262059	6	30	theme	significant	978:988	arg1	increase					990:997	a significant increase	976:997	a significant increase in the amount of perlecan in the prostate stroma	976:1046	There was a significant increase in the amount of perlecan in the prostate stroma from groups subjected to castration plus flutamide for 7 or 30 days.					
36262059	2	31	theme	antiandrogen	413:424	arg1	10 mg/kg/day					447:458	flutamide 10 mg/kg/day	437:458	flutamide 10 mg/kg/day	437:458	METHODS The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals.					
36262059	2	31	theme	antiandrogen	413:424	arg1	treatment					426:434	antiandrogen treatment	413:434	antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period	413:505	METHODS The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals.					
36262059	0	32	theme	cytoarchitecture	50:65	arg1	remodeling					26:35	remodeling	26:35	remodeling of prostatic cytoarchitecture	26:65	Proteoglycans orchestrate remodeling of prostatic cytoarchitecture after androgenic blockade in old gerbils.					
36262059	6	33	theme	perlecan	1016:1023	arg1	amount					1006:1011	the amount	1002:1011	the amount of perlecan	1002:1023	There was a significant increase in the amount of perlecan in the prostate stroma from groups subjected to castration plus flutamide for 7 or 30 days.					
36262059	6	33	theme	perlecan	1016:1023	arg1	perlecan					1016:1023	perlecan	1016:1023	perlecan	1016:1023	There was a significant increase in the amount of perlecan in the prostate stroma from groups subjected to castration plus flutamide for 7 or 30 days.					
36262059	11	34	from	alterations	1777:1787	arg1	associations					1817:1828	associations	1817:1828	proteoglycans as well as associations among them and with the basement membrane	1792:1870	Length and form alterations in proteoglycans as well as associations among them and with the basement membrane were dynamic events in the prostate microenvironment.					
36262059	11	34	from	alterations	1777:1787	arg1	proteoglycans					1792:1804	proteoglycans	1792:1804	proteoglycans as well as associations among them and with the basement membrane	1792:1870	Length and form alterations in proteoglycans as well as associations among them and with the basement membrane were dynamic events in the prostate microenvironment.					
36262059	10	35	theme	perlecan	1679:1686	arg1	permanence					1665:1674	permanence	1665:1674	permanence	1665:1674	CONCLUSIONS In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent.					
36262059	10	35	theme	perlecan	1679:1686	arg1	frequency					1651:1659	high frequency	1646:1659	high frequency	1646:1659	CONCLUSIONS In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent.					
36262059	1	36	theme	prostatic	222:230	arg1	stroma					232:237	the prostatic stroma	218:237	the prostatic stroma of 18-month-old gerbils	218:261	BACKGROUND The aim of this study was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade.					
36262059	0	37	theme	prostatic	40:48	arg1	cytoarchitecture					50:65	prostatic cytoarchitecture	40:65	prostatic cytoarchitecture	40:65	Proteoglycans orchestrate remodeling of prostatic cytoarchitecture after androgenic blockade in old gerbils.					
36262059	11	38	theme	dynamic	1877:1883	arg1	events					1885:1890	dynamic events	1877:1890	dynamic events in the prostate microenvironment	1877:1923	Length and form alterations in proteoglycans as well as associations among them and with the basement membrane were dynamic events in the prostate microenvironment.					
36262059	8	39	theme	androgenic	1264:1273	arg1	blockade					1275:1282	androgenic blockade	1264:1282	androgenic blockade	1264:1282	In addition, androgenic blockade results in changes in the amount, thickness, and morphology of these structures.					
36262059	11	40	theme	form	1772:1775	arg1	alterations					1777:1787	Length and form alterations	1761:1787	Length and form alterations in proteoglycans as well as associations among them and with the basement membrane	1761:1870	Length and form alterations in proteoglycans as well as associations among them and with the basement membrane were dynamic events in the prostate microenvironment.					
36262059	0	41	theme	androgenic	73:82	arg1	blockade					84:91	androgenic blockade	73:91	androgenic blockade in old gerbils	73:106	Proteoglycans orchestrate remodeling of prostatic cytoarchitecture after androgenic blockade in old gerbils.					
36262059	1	42	theme	androgenic	320:329	arg1	blockade					331:338	an androgenic blockade	317:338	an androgenic blockade	317:338	BACKGROUND The aim of this study was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade.					
36262059	0	43	from	blockade	84:91	arg1	gerbils					100:106	old gerbils	96:106	old gerbils	96:106	Proteoglycans orchestrate remodeling of prostatic cytoarchitecture after androgenic blockade in old gerbils.					
36262059	11	44	with	associations	1817:1828	arg1	membrane					1863:1870	the basement membrane	1850:1870	the basement membrane	1850:1870	Length and form alterations in proteoglycans as well as associations among them and with the basement membrane were dynamic events in the prostate microenvironment.					
36262059	11	45	theme	prostate	1899:1906	arg1	microenvironment					1908:1923	the prostate microenvironment	1895:1923	the prostate microenvironment	1895:1923	Length and form alterations in proteoglycans as well as associations among them and with the basement membrane were dynamic events in the prostate microenvironment.					
36262059	10	46	theme	chondroitin	1521:1531	arg1	sulfate					1533:1539	chondroitin sulfate	1521:1539	chondroitin sulfate	1521:1539	CONCLUSIONS In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent.					
36262059	2	47	theme	intact	585:590	arg1	animals					592:598	intact animals	585:598	intact animals	585:598	METHODS The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals.					
36262059	3	48	theme	chondroitin	671:681	arg1	sulfate					683:689	chondroitin sulfate	671:689	chondroitin sulfate	671:689	Tissue analysis included immunohistochemical assessment (perlecan and chondroitin sulfate) and proteoglycan morphology was analyzed by transmission electron microscopy.					
36262059	3	49	theme	Tissue	601:606	arg1	analysis					608:615	Tissue analysis	601:615	Tissue analysis	601:615	Tissue analysis included immunohistochemical assessment (perlecan and chondroitin sulfate) and proteoglycan morphology was analyzed by transmission electron microscopy.					
36262059	10	50	from	decrease	1509:1516	arg1	sulfate					1533:1539	chondroitin sulfate	1521:1539	chondroitin sulfate	1521:1539	CONCLUSIONS In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent.					
36262059	4	51	theme	androgenic	852:861	arg1	blockade					863:870	an androgenic blockade	849:870	an androgenic blockade	849:870	RESULTS Chondroitin sulfate frequency was increased 7 days postcastration with an androgenic blockade.					
36262059	2	52	dep	groups	386:391	arg1	subjected					393:401	subjected	393:401	subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period	393:505	METHODS The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals.					
36262059	2	52	dep	groups	386:391	arg1	euthanized					511:520	euthanized	511:520	euthanized at 7- or 30-day postcastration	511:551	METHODS The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals.					
36262059	8	53	from	changes	1295:1301	arg1	structures					1353:1362	these structures	1347:1362	these structures	1347:1362	In addition, androgenic blockade results in changes in the amount, thickness, and morphology of these structures.					
36262059	8	53	from	changes	1295:1301	arg1	thickness					1318:1326	thickness	1318:1326	thickness	1318:1326	In addition, androgenic blockade results in changes in the amount, thickness, and morphology of these structures.					
36262059	8	53	from	changes	1295:1301	arg1	morphology					1333:1342	morphology	1333:1342	morphology	1333:1342	In addition, androgenic blockade results in changes in the amount, thickness, and morphology of these structures.					
36262059	8	53	from	changes	1295:1301	arg1	amount					1310:1315	amount	1310:1315	amount	1310:1315	In addition, androgenic blockade results in changes in the amount, thickness, and morphology of these structures.					
36262059	1	54	theme	study	136:140	arg1	aim					124:126	The aim	120:126	The aim of this study	120:140	BACKGROUND The aim of this study was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade.					
36262059	10	55	from	CONCLUSIONS	1463:1473	arg1	study					1483:1487	this study	1478:1487	this study	1478:1487	CONCLUSIONS In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent.					
36262059	9	56	theme	larger	1429:1434	arg1	proteoglycans					1436:1448	larger proteoglycans	1429:1448	larger proteoglycans	1429:1448	At 30 days postcastration, with or without flutamide treatment, larger proteoglycans were common.					
36262059	10	57	theme	longer	1551:1556	arg1	period					1558:1563	the longer period	1547:1563	the longer period	1547:1563	CONCLUSIONS In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent.					
36262059	11	58	theme	Length	1761:1766	arg1	alterations					1777:1787	Length and form alterations	1761:1787	Length and form alterations in proteoglycans as well as associations among them and with the basement membrane	1761:1870	Length and form alterations in proteoglycans as well as associations among them and with the basement membrane were dynamic events in the prostate microenvironment.					
36262059	11	59	theme	basement	1854:1861	arg1	membrane					1863:1870	the basement membrane	1850:1870	the basement membrane	1850:1870	Length and form alterations in proteoglycans as well as associations among them and with the basement membrane were dynamic events in the prostate microenvironment.					
36262059	4	60	dep	RESULTS	770:776	arg1	increased					812:820	increased	812:820	was increased 7 days postcastration with an androgenic blockade	808:870	RESULTS Chondroitin sulfate frequency was increased 7 days postcastration with an androgenic blockade.					
36262059	1	61	theme	18-month-old	242:253	arg1	gerbils					255:261	18-month-old gerbils	242:261	18-month-old gerbils	242:261	BACKGROUND The aim of this study was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade.					
36262059	10	62	theme	prostatic	1590:1598	arg1	CONCLUSIONS					1463:1473	CONCLUSIONS	1463:1473	CONCLUSIONS In this study	1463:1487	CONCLUSIONS In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent.					
36262059	10	62	theme	prostatic	1590:1598	arg1	decrease					1509:1516	the decrease	1505:1516	the decrease in chondroitin sulfate after the longer period	1505:1563	CONCLUSIONS In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent.					
36262059	10	62	theme	prostatic	1590:1598	arg1	response					1600:1607	a prostatic response	1588:1607	a prostatic response to androgenic deprivation	1588:1633	CONCLUSIONS In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent.					
36262059	3	63	theme	proteoglycan	696:707	arg1	morphology					709:718	proteoglycan morphology	696:718	proteoglycan morphology	696:718	Tissue analysis included immunohistochemical assessment (perlecan and chondroitin sulfate) and proteoglycan morphology was analyzed by transmission electron microscopy.					
36262059	3	64	theme	immunohistochemical	626:644	arg1	assessment					646:655	immunohistochemical assessment	626:655	immunohistochemical assessment (perlecan and chondroitin sulfate)	626:690	Tissue analysis included immunohistochemical assessment (perlecan and chondroitin sulfate) and proteoglycan morphology was analyzed by transmission electron microscopy.					
36262059	11	65	with	proteoglycans	1792:1804	arg1	membrane					1863:1870	the basement membrane	1850:1870	the basement membrane	1850:1870	Length and form alterations in proteoglycans as well as associations among them and with the basement membrane were dynamic events in the prostate microenvironment.					
36262059	5	66	theme	blockade	946:953	arg1	treatment					955:963	androgenic blockade treatment	935:963	androgenic blockade treatment	935:963	The presence of these carbohydrates was rare after 30 days of androgenic blockade treatment.					
36262059	1	67	theme	gerbils	255:261	arg1	stroma					232:237	the prostatic stroma	218:237	the prostatic stroma of 18-month-old gerbils	218:261	BACKGROUND The aim of this study was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade.					
36262059	3	68	theme	transmission	736:747	arg1	microscopy					758:767	transmission electron microscopy	736:767	transmission electron microscopy	736:767	Tissue analysis included immunohistochemical assessment (perlecan and chondroitin sulfate) and proteoglycan morphology was analyzed by transmission electron microscopy.					
36262059	5	69	theme	treatment	955:963	arg1	days					927:930	30 days	924:930	30 days of androgenic blockade treatment	924:963	The presence of these carbohydrates was rare after 30 days of androgenic blockade treatment.					
36262059	9	70	theme	flutamide	1408:1416	arg1	treatment					1418:1426	flutamide treatment	1408:1426	flutamide treatment	1408:1426	At 30 days postcastration, with or without flutamide treatment, larger proteoglycans were common.					
35326419	0	0	theme	Alcohol	84:90	arg1	Disease					98:104	Alcohol Liver Disease	84:104	Alcohol Liver Disease	84:104	Modulation of the Bile Acid Enterohepatic Cycle by Intestinal Microbiota Alleviates Alcohol Liver Disease.					
35326419	8	1	theme	farnesoid	1093:1101	arg1	receptor					1105:1112	farnesoid X receptor	1093:1112	farnesoid X receptor signaling in the ileum	1093:1135	The modulation of BA content by pectin inhibited farnesoid X receptor signaling in the ileum and the subsequent upregulation of Cyp7a1 in the liver.					
35326419	5	2	theme	overall	715:721	arg1	composition					726:736	the overall BA composition	711:736	the overall BA composition	711:736	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	4	3	from	levels	583:588	arg1	liver					608:612	liver	608:612	liver	608:612	This beneficial effect correlated with lower BA levels in the plasma and liver but higher levels in the caecum, suggesting that pectin stimulated BA excretion.					
35326419	4	3	from	levels	583:588	arg1	levels					625:630	higher levels	618:630	higher levels in the caecum	618:644	This beneficial effect correlated with lower BA levels in the plasma and liver but higher levels in the caecum, suggesting that pectin stimulated BA excretion.					
35326419	4	3	from	levels	583:588	arg1	plasma					597:602	plasma	597:602	plasma	597:602	This beneficial effect correlated with lower BA levels in the plasma and liver but higher levels in the caecum, suggesting that pectin stimulated BA excretion.					
35326419	2	4	from	metabolism	403:412	arg1	mice					436:439	alcohol-challenged mice	417:439	alcohol-challenged mice receiving feces from patients with alcoholic hepatitis	417:494	In this context, we aimed to elucidate how oral supplementation of pectin affects BA metabolism in alcohol-challenged mice receiving feces from patients with alcoholic hepatitis.					
35326419	10	5	theme	alcohol	1335:1341	arg1	disease					1349:1355	alcohol liver disease	1335:1355	alcohol liver disease	1335:1355	In conclusion, pectin alleviates alcohol liver disease by modifying the BA cycle through effects on the intestinal microbiota and enhanced BA excretion.					
35326419	1	6	theme	fiber	163:167	arg1	ingestion					150:158	the ingestion	146:158	the ingestion of fiber, such as pectin,	146:184	Reshaping the intestinal microbiota by the ingestion of fiber, such as pectin, improves alcohol-induced liver lesions in mice by modulating bacterial metabolites, including indoles, as well as bile acids (BAs).					
35326419	1	7	theme	bacterial	247:255	arg1	metabolites					257:267	bacterial metabolites	247:267	bacterial metabolites	247:267	Reshaping the intestinal microbiota by the ingestion of fiber, such as pectin, improves alcohol-induced liver lesions in mice by modulating bacterial metabolites, including indoles, as well as bile acids (BAs).					
35326419	8	8	theme	receptor	1105:1112	arg1	signaling					1114:1122	farnesoid X receptor signaling	1093:1122	farnesoid X receptor signaling in the ileum	1093:1135	The modulation of BA content by pectin inhibited farnesoid X receptor signaling in the ileum and the subsequent upregulation of Cyp7a1 in the liver.					
35326419	5	9	from	augmentation	751:762	arg1	proportion					771:780	the proportion	767:780	the proportion of hydrophilic forms in the liver, plasma, and gut	767:831	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	10	10	theme	BA	1374:1375	arg1	cycle					1377:1381	the BA cycle	1370:1381	the BA cycle	1370:1381	In conclusion, pectin alleviates alcohol liver disease by modifying the BA cycle through effects on the intestinal microbiota and enhanced BA excretion.					
35326419	3	11	theme	liver	520:524	arg1	disease					526:532	alcohol liver disease	512:532	alcohol liver disease	512:532	Pectin reduced alcohol liver disease.					
35326419	4	12	theme	BA	580:581	arg1	levels					583:588	lower BA levels	574:588	lower BA levels in the plasma and liver but higher levels in the caecum	574:644	This beneficial effect correlated with lower BA levels in the plasma and liver but higher levels in the caecum, suggesting that pectin stimulated BA excretion.					
35326419	0	13	theme	Liver	92:96	arg1	Disease					98:104	Alcohol Liver Disease	84:104	Alcohol Liver Disease	84:104	Modulation of the Bile Acid Enterohepatic Cycle by Intestinal Microbiota Alleviates Alcohol Liver Disease.					
35326419	4	14	theme	lower	574:578	arg1	levels					583:588	lower BA levels	574:588	lower BA levels in the plasma and liver but higher levels in the caecum	574:644	This beneficial effect correlated with lower BA levels in the plasma and liver but higher levels in the caecum, suggesting that pectin stimulated BA excretion.					
35326419	5	15	from	forms	797:801	arg1	liver					810:814	liver	810:814	liver	810:814	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	5	15	from	forms	797:801	arg1	plasma					817:822	plasma	817:822	plasma	817:822	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	5	15	from	forms	797:801	arg1	gut					829:831	gut	829:831	gut	829:831	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	10	16	theme	intestinal	1406:1415	arg1	microbiota					1417:1426	the intestinal microbiota	1402:1426	the intestinal microbiota	1402:1426	In conclusion, pectin alleviates alcohol liver disease by modifying the BA cycle through effects on the intestinal microbiota and enhanced BA excretion.					
35326419	2	17	with	patients	462:469	arg1	hepatitis					486:494	alcoholic hepatitis	476:494	alcoholic hepatitis	476:494	In this context, we aimed to elucidate how oral supplementation of pectin affects BA metabolism in alcohol-challenged mice receiving feces from patients with alcoholic hepatitis.					
35326419	2	18	theme	alcoholic	476:484	arg1	hepatitis					486:494	alcoholic hepatitis	476:494	alcoholic hepatitis	476:494	In this context, we aimed to elucidate how oral supplementation of pectin affects BA metabolism in alcohol-challenged mice receiving feces from patients with alcoholic hepatitis.					
35326419	5	19	from	proportion	771:780	arg1	liver					810:814	liver	810:814	liver	810:814	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	5	19	from	proportion	771:780	arg1	plasma					817:822	plasma	817:822	plasma	817:822	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	5	19	from	proportion	771:780	arg1	gut					829:831	gut	829:831	gut	829:831	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	6	20	from	BAs	922:924	arg1	gut					933:935	the gut	929:935	the gut	929:935	This effect was linked to an imbalance between hydrophobic and hydrophilic (less toxic) BAs in the gut.					
35326419	3	21	theme	alcohol	512:518	arg1	disease					526:532	alcohol liver disease	512:532	alcohol liver disease	512:532	Pectin reduced alcohol liver disease.					
35326419	6	22	theme	hydrophobic	881:891	arg1	BAs					922:924	hydrophobic and hydrophilic (less toxic) BAs	881:924	hydrophobic and hydrophilic (less toxic) BAs in the gut	881:935	This effect was linked to an imbalance between hydrophobic and hydrophilic (less toxic) BAs in the gut.					
35326419	9	23	theme	intestinal	1280:1289	arg1	excretion					1291:1299	their intestinal excretion	1274:1299	their intestinal excretion	1274:1299	Despite an increase in BA synthesis, pectin reduced BA serum levels by promoting their intestinal excretion.					
35326419	7	24	theme	BA-metabolizing	1019:1033	arg1	genes					1001:1005	genes	1001:1005	genes that encode BA-metabolizing enzymes	1001:1041	Pectin induced the enrichment of intestinal bacteria harboring genes that encode BA-metabolizing enzymes.					
35326419	7	24	theme	BA-metabolizing	1019:1033	arg1	enzymes					1035:1041	BA-metabolizing enzymes	1019:1041	BA-metabolizing enzymes	1019:1041	Pectin induced the enrichment of intestinal bacteria harboring genes that encode BA-metabolizing enzymes.					
35326419	9	25	from	increase	1204:1211	arg1	synthesis					1219:1227	BA synthesis	1216:1227	BA synthesis	1216:1227	Despite an increase in BA synthesis, pectin reduced BA serum levels by promoting their intestinal excretion.					
35326419	4	26	from	levels	625:630	arg1	caecum					639:644	the caecum	635:644	the caecum	635:644	This beneficial effect correlated with lower BA levels in the plasma and liver but higher levels in the caecum, suggesting that pectin stimulated BA excretion.					
35326419	4	27	theme	higher	618:623	arg1	levels					625:630	higher levels	618:630	higher levels in the caecum	618:644	This beneficial effect correlated with lower BA levels in the plasma and liver but higher levels in the caecum, suggesting that pectin stimulated BA excretion.					
35326419	2	28	theme	BA	400:401	arg1	metabolism					403:412	BA metabolism	400:412	BA metabolism in alcohol-challenged mice receiving feces from patients with alcoholic hepatitis	400:494	In this context, we aimed to elucidate how oral supplementation of pectin affects BA metabolism in alcohol-challenged mice receiving feces from patients with alcoholic hepatitis.					
35326419	0	29	theme	Bile	18:21	arg1	Cycle					42:46	the Bile Acid Enterohepatic Cycle	14:46	the Bile Acid Enterohepatic Cycle	14:46	Modulation of the Bile Acid Enterohepatic Cycle by Intestinal Microbiota Alleviates Alcohol Liver Disease.					
35326419	10	30	theme	BA	1441:1442	arg1	excretion					1444:1452	enhanced BA excretion	1432:1452	enhanced BA excretion	1432:1452	In conclusion, pectin alleviates alcohol liver disease by modifying the BA cycle through effects on the intestinal microbiota and enhanced BA excretion.					
35326419	10	31	theme	liver	1343:1347	arg1	disease					1349:1355	alcohol liver disease	1335:1355	alcohol liver disease	1335:1355	In conclusion, pectin alleviates alcohol liver disease by modifying the BA cycle through effects on the intestinal microbiota and enhanced BA excretion.					
35326419	2	32	theme	pectin	385:390	arg1	supplementation					366:380	oral supplementation	361:380	oral supplementation of pectin	361:390	In this context, we aimed to elucidate how oral supplementation of pectin affects BA metabolism in alcohol-challenged mice receiving feces from patients with alcoholic hepatitis.					
35326419	10	33	theme	enhanced	1432:1439	arg1	excretion					1444:1452	enhanced BA excretion	1432:1452	enhanced BA excretion	1432:1452	In conclusion, pectin alleviates alcohol liver disease by modifying the BA cycle through effects on the intestinal microbiota and enhanced BA excretion.					
35326419	6	34	dep	hydrophobic	881:891	arg1	toxic					915:919	toxic	915:919	toxic	915:919	This effect was linked to an imbalance between hydrophobic and hydrophilic (less toxic) BAs in the gut.					
35326419	0	35	theme	Enterohepatic	28:40	arg1	Cycle					42:46	the Bile Acid Enterohepatic Cycle	14:46	the Bile Acid Enterohepatic Cycle	14:46	Modulation of the Bile Acid Enterohepatic Cycle by Intestinal Microbiota Alleviates Alcohol Liver Disease.					
35326419	4	36	theme	beneficial	540:549	arg1	effect					551:556	This beneficial effect	535:556	This beneficial effect	535:556	This beneficial effect correlated with lower BA levels in the plasma and liver but higher levels in the caecum, suggesting that pectin stimulated BA excretion.					
35326419	9	37	theme	BA	1245:1246	arg1	levels					1254:1259	BA serum levels	1245:1259	BA serum levels	1245:1259	Despite an increase in BA synthesis, pectin reduced BA serum levels by promoting their intestinal excretion.					
35326419	1	38	theme	alcohol-induced	195:209	arg1	lesions					217:223	alcohol-induced liver lesions	195:223	alcohol-induced liver lesions in mice	195:231	Reshaping the intestinal microbiota by the ingestion of fiber, such as pectin, improves alcohol-induced liver lesions in mice by modulating bacterial metabolites, including indoles, as well as bile acids (BAs).					
35326419	2	39	theme	oral	361:364	arg1	supplementation					366:380	oral supplementation	361:380	oral supplementation of pectin	361:390	In this context, we aimed to elucidate how oral supplementation of pectin affects BA metabolism in alcohol-challenged mice receiving feces from patients with alcoholic hepatitis.					
35326419	0	40	theme	Acid	23:26	arg1	Cycle					42:46	the Bile Acid Enterohepatic Cycle	14:46	the Bile Acid Enterohepatic Cycle	14:46	Modulation of the Bile Acid Enterohepatic Cycle by Intestinal Microbiota Alleviates Alcohol Liver Disease.					
35326419	6	41	theme	hydrophilic	897:907	arg1	BAs					922:924	hydrophobic and hydrophilic (less toxic) BAs	881:924	hydrophobic and hydrophilic (less toxic) BAs in the gut	881:935	This effect was linked to an imbalance between hydrophobic and hydrophilic (less toxic) BAs in the gut.					
35326419	8	42	theme	subsequent	1145:1154	arg1	upregulation					1156:1167	the subsequent upregulation	1141:1167	the subsequent upregulation of Cyp7a1 in the liver	1141:1190	The modulation of BA content by pectin inhibited farnesoid X receptor signaling in the ileum and the subsequent upregulation of Cyp7a1 in the liver.					
35326419	5	43	theme	hydrophilic	785:795	arg1	forms					797:801	hydrophilic forms	785:801	hydrophilic forms in the liver, plasma, and gut	785:831	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	5	44	from	liver	810:814	arg1	proportion					771:780	the proportion	767:780	the proportion of hydrophilic forms in the liver, plasma, and gut	767:831	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	1	45	theme	liver	211:215	arg1	lesions					217:223	alcohol-induced liver lesions	195:223	alcohol-induced liver lesions in mice	195:231	Reshaping the intestinal microbiota by the ingestion of fiber, such as pectin, improves alcohol-induced liver lesions in mice by modulating bacterial metabolites, including indoles, as well as bile acids (BAs).					
35326419	2	46	theme	alcohol-challenged	417:434	arg1	mice					436:439	alcohol-challenged mice	417:439	alcohol-challenged mice receiving feces from patients with alcoholic hepatitis	417:494	In this context, we aimed to elucidate how oral supplementation of pectin affects BA metabolism in alcohol-challenged mice receiving feces from patients with alcoholic hepatitis.					
35326419	10	47	from	effects	1391:1397	arg1	microbiota					1417:1426	the intestinal microbiota	1402:1426	the intestinal microbiota	1402:1426	In conclusion, pectin alleviates alcohol liver disease by modifying the BA cycle through effects on the intestinal microbiota and enhanced BA excretion.					
35326419	10	47	from	effects	1391:1397	arg1	excretion					1444:1452	enhanced BA excretion	1432:1452	enhanced BA excretion	1432:1452	In conclusion, pectin alleviates alcohol liver disease by modifying the BA cycle through effects on the intestinal microbiota and enhanced BA excretion.					
35326419	7	48	theme	intestinal	971:980	arg1	bacteria					982:989	intestinal bacteria	971:989	intestinal bacteria harboring genes that encode BA-metabolizing enzymes	971:1041	Pectin induced the enrichment of intestinal bacteria harboring genes that encode BA-metabolizing enzymes.					
35326419	8	49	theme	content	1065:1071	arg1	modulation					1048:1057	The modulation	1044:1057	The modulation of BA content by pectin	1044:1081	The modulation of BA content by pectin inhibited farnesoid X receptor signaling in the ileum and the subsequent upregulation of Cyp7a1 in the liver.					
35326419	4	50	theme	BA	681:682	arg1	excretion					684:692	BA excretion	681:692	BA excretion	681:692	This beneficial effect correlated with lower BA levels in the plasma and liver but higher levels in the caecum, suggesting that pectin stimulated BA excretion.					
35326419	0	51	theme	Cycle	42:46	arg1	Modulation					0:9	Modulation	0:9	Modulation of the Bile Acid Enterohepatic Cycle by Intestinal Microbiota	0:71	Modulation of the Bile Acid Enterohepatic Cycle by Intestinal Microbiota Alleviates Alcohol Liver Disease.					
35326419	1	52	theme	bile	300:303	arg1	BAs					312:314	BAs	312:314	BAs	312:314	Reshaping the intestinal microbiota by the ingestion of fiber, such as pectin, improves alcohol-induced liver lesions in mice by modulating bacterial metabolites, including indoles, as well as bile acids (BAs).					
35326419	1	52	theme	bile	300:303	arg1	acids					305:309	bile acids	300:309	bile acids (BAs)	300:315	Reshaping the intestinal microbiota by the ingestion of fiber, such as pectin, improves alcohol-induced liver lesions in mice by modulating bacterial metabolites, including indoles, as well as bile acids (BAs).					
35326419	7	53	theme	bacteria	982:989	arg1	enrichment					957:966	the enrichment	953:966	the enrichment of intestinal bacteria harboring genes that encode BA-metabolizing enzymes	953:1041	Pectin induced the enrichment of intestinal bacteria harboring genes that encode BA-metabolizing enzymes.					
35326419	6	54	attach	linked	850:855	arg1	imbalance					863:871	an imbalance	860:871	an imbalance between hydrophobic and hydrophilic (less toxic) BAs in the gut	860:935	This effect was linked to an imbalance between hydrophobic and hydrophilic (less toxic) BAs in the gut.					
35326419	6	54	attach	linked	850:855	arg2	effect					839:844	This effect	834:844	This effect	834:844	This effect was linked to an imbalance between hydrophobic and hydrophilic (less toxic) BAs in the gut.					
35326419	1	55	theme	intestinal	121:130	arg1	microbiota					132:141	the intestinal microbiota	117:141	the intestinal microbiota	117:141	Reshaping the intestinal microbiota by the ingestion of fiber, such as pectin, improves alcohol-induced liver lesions in mice by modulating bacterial metabolites, including indoles, as well as bile acids (BAs).					
35326419	9	56	theme	serum	1248:1252	arg1	levels					1254:1259	BA serum levels	1245:1259	BA serum levels	1245:1259	Despite an increase in BA synthesis, pectin reduced BA serum levels by promoting their intestinal excretion.					
35326419	1	57	from	lesions	217:223	arg1	mice					228:231	mice	228:231	mice	228:231	Reshaping the intestinal microbiota by the ingestion of fiber, such as pectin, improves alcohol-induced liver lesions in mice by modulating bacterial metabolites, including indoles, as well as bile acids (BAs).					
35326419	8	58	from	signaling	1114:1122	arg1	liver					1186:1190	the liver	1182:1190	the liver	1182:1190	The modulation of BA content by pectin inhibited farnesoid X receptor signaling in the ileum and the subsequent upregulation of Cyp7a1 in the liver.					
35326419	8	58	from	signaling	1114:1122	arg1	ileum					1131:1135	the ileum	1127:1135	the ileum	1127:1135	The modulation of BA content by pectin inhibited farnesoid X receptor signaling in the ileum and the subsequent upregulation of Cyp7a1 in the liver.					
35326419	8	59	theme	BA	1062:1063	arg1	content					1065:1071	BA content	1062:1071	BA content	1062:1071	The modulation of BA content by pectin inhibited farnesoid X receptor signaling in the ileum and the subsequent upregulation of Cyp7a1 in the liver.					
35326419	8	60	theme	Cyp7a1	1172:1177	arg1	upregulation					1156:1167	the subsequent upregulation	1141:1167	the subsequent upregulation of Cyp7a1 in the liver	1141:1190	The modulation of BA content by pectin inhibited farnesoid X receptor signaling in the ileum and the subsequent upregulation of Cyp7a1 in the liver.					
35326419	8	60	theme	Cyp7a1	1172:1177	arg1	signaling					1114:1122	farnesoid X receptor signaling	1093:1122	farnesoid X receptor signaling in the ileum	1093:1135	The modulation of BA content by pectin inhibited farnesoid X receptor signaling in the ileum and the subsequent upregulation of Cyp7a1 in the liver.					
35326419	5	61	theme	BA	723:724	arg1	composition					726:736	the overall BA composition	711:736	the overall BA composition	711:736	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	5	62	from	gut	829:831	arg1	proportion					771:780	the proportion	767:780	the proportion of hydrophilic forms in the liver, plasma, and gut	767:831	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	8	63	from	upregulation	1156:1167	arg1	liver					1186:1190	the liver	1182:1190	the liver	1182:1190	The modulation of BA content by pectin inhibited farnesoid X receptor signaling in the ileum and the subsequent upregulation of Cyp7a1 in the liver.					
35326419	8	63	from	upregulation	1156:1167	arg1	ileum					1131:1135	the ileum	1127:1135	the ileum	1127:1135	The modulation of BA content by pectin inhibited farnesoid X receptor signaling in the ileum and the subsequent upregulation of Cyp7a1 in the liver.					
35326419	0	64	theme	Intestinal	51:60	arg1	Microbiota					62:71	Intestinal Microbiota	51:71	Intestinal Microbiota	51:71	Modulation of the Bile Acid Enterohepatic Cycle by Intestinal Microbiota Alleviates Alcohol Liver Disease.					
35326419	9	65	theme	BA	1216:1217	arg1	synthesis					1219:1227	BA synthesis	1216:1227	BA synthesis	1216:1227	Despite an increase in BA synthesis, pectin reduced BA serum levels by promoting their intestinal excretion.					
35326419	5	66	theme	forms	797:801	arg1	proportion					771:780	the proportion	767:780	the proportion of hydrophilic forms in the liver, plasma, and gut	767:831	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	5	67	from	plasma	817:822	arg1	proportion					771:780	the proportion	767:780	the proportion of hydrophilic forms in the liver, plasma, and gut	767:831	Pectin modified the overall BA composition, favoring an augmentation in the proportion of hydrophilic forms in the liver, plasma, and gut.					
35326419	8	68	theme	X	1103:1103	arg1	receptor					1105:1112	farnesoid X receptor	1093:1112	farnesoid X receptor signaling in the ileum	1093:1135	The modulation of BA content by pectin inhibited farnesoid X receptor signaling in the ileum and the subsequent upregulation of Cyp7a1 in the liver.					
37110788	0	0	theme	Acid	98:101	arg1	Pathway					103:109	the Hyaluronic Acid Pathway	83:109	the Hyaluronic Acid Pathway	83:109	Unsaturated Fatty Acids Complex Regulates Inflammatory Cytokine Production through the Hyaluronic Acid Pathway.					
37110788	8	1	theme	lipopolysaccharide	1184:1201	arg1	treatment					1209:1217	lipopolysaccharide (LPS) treatment	1184:1217	lipopolysaccharide (LPS) treatment	1184:1217	The secretion of all the cytokines was increased by lipopolysaccharide (LPS) treatment; however, this was inhibited by the EUFOC in a concentration-dependent manner.					
37110788	1	2	theme	natural	147:153	arg1	materials					173:181	natural and/or functional materials	147:181	natural and/or functional materials with antioxidant and anti-inflammatory effects	147:228	In this study, we aimed to develop natural and/or functional materials with antioxidant and anti-inflammatory effects.					
37110788	0	3	theme	Hyaluronic	87:96	arg1	Pathway					103:109	the Hyaluronic Acid Pathway	83:109	the Hyaluronic Acid Pathway	83:109	Unsaturated Fatty Acids Complex Regulates Inflammatory Cytokine Production through the Hyaluronic Acid Pathway.					
37110788	9	4	theme	acid	1322:1325	arg1	content					1327:1333	hyaluronic acid content	1311:1333	hyaluronic acid content	1311:1333	In addition, hyaluronic acid content was markedly increased by the EUFOC in a dose-dependent manner.					
37110788	5	5	theme	keratinocyte	902:913	arg1	cells					916:920	HaCaT (human keratinocyte) cells	889:920	HaCaT (human keratinocyte) cells	889:920	In addition, it showed no endogenous cytotoxicity in HaCaT (human keratinocyte) cells.					
37110788	2	6	theme	effective	360:368	arg1	EUFOC					402:406	EUFOC	402:406	EUFOC	402:406	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	2	6	theme	effective	360:368	arg1	acid					388:391	an effective unsaturated fatty acid complex	357:399	an effective unsaturated fatty acid complex (EUFOC)	357:407	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	10	7	contain	has	1436:1438	arg1	EUFOC					1430:1434	the EUFOC	1426:1434	the EUFOC	1426:1434	These results suggest that the EUFOC has excellent anti-inflammatory and antioxidant properties, and hence, it can be used as a functional material in various fields.					
37110788	10	7	contain	has	1436:1438	arg2	properties					1484:1493	excellent anti-inflammatory and antioxidant properties	1440:1493	excellent anti-inflammatory and antioxidant properties	1440:1493	These results suggest that the EUFOC has excellent anti-inflammatory and antioxidant properties, and hence, it can be used as a functional material in various fields.					
37110788	10	8	theme	excellent	1440:1448	arg1	properties					1484:1493	excellent anti-inflammatory and antioxidant properties	1440:1493	excellent anti-inflammatory and antioxidant properties	1440:1493	These results suggest that the EUFOC has excellent anti-inflammatory and antioxidant properties, and hence, it can be used as a functional material in various fields.					
37110788	10	9	theme	antioxidant	1472:1482	arg1	properties					1484:1493	excellent anti-inflammatory and antioxidant properties	1440:1493	excellent anti-inflammatory and antioxidant properties	1440:1493	These results suggest that the EUFOC has excellent anti-inflammatory and antioxidant properties, and hence, it can be used as a functional material in various fields.					
37110788	2	10	theme	extraction	301:310	arg1	process					312:318	an oil and hot-water extraction process	280:318	an oil and hot-water extraction process	280:318	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	3	11	from	effect	558:563	arg1	production					581:590	nitric oxide production	568:590	nitric oxide production	568:590	Furthermore, the antioxidant effect of the extract complex was evaluated, and the anti-inflammatory effect was explored by assessing its inhibitory effect on nitric oxide production through its HA-promoting effect.					
37110788	2	12	theme	hot-water	291:299	arg1	process					312:318	an oil and hot-water extraction process	280:318	an oil and hot-water extraction process	280:318	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	4	13	theme	EUFOC	749:753	arg1	viability					732:740	the cell viability	723:740	the cell viability of the EUFOC	723:753	We conducted a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay to evaluate the cell viability of the EUFOC, and the results showed that EUFOC was not cytotoxic at the test concentrations.					
37110788	4	14	theme	cell	727:730	arg1	viability					732:740	the cell viability	723:740	the cell viability of the EUFOC	723:753	We conducted a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay to evaluate the cell viability of the EUFOC, and the results showed that EUFOC was not cytotoxic at the test concentrations.					
37110788	7	15	from	effect	1053:1058	arg1	production					1066:1075	NO production	1063:1075	NO production	1063:1075	Moreover, it exerted an inhibitory effect on NO production at concentrations that did not inhibit cell viability.					
37110788	8	16	theme	concentration-dependent	1266:1288	arg1	manner					1290:1295	a concentration-dependent manner	1264:1295	a concentration-dependent manner	1264:1295	The secretion of all the cytokines was increased by lipopolysaccharide (LPS) treatment; however, this was inhibited by the EUFOC in a concentration-dependent manner.					
37110788	1	17	theme	functional	162:171	arg1	materials					173:181	natural and/or functional materials	147:181	natural and/or functional materials with antioxidant and anti-inflammatory effects	147:228	In this study, we aimed to develop natural and/or functional materials with antioxidant and anti-inflammatory effects.					
37110788	10	18	from	material	1538:1545	arg1	fields					1558:1563	various fields	1550:1563	various fields	1550:1563	These results suggest that the EUFOC has excellent anti-inflammatory and antioxidant properties, and hence, it can be used as a functional material in various fields.					
37110788	10	19	theme	anti-inflammatory	1450:1466	arg1	properties					1484:1493	excellent anti-inflammatory and antioxidant properties	1440:1493	excellent anti-inflammatory and antioxidant properties	1440:1493	These results suggest that the EUFOC has excellent anti-inflammatory and antioxidant properties, and hence, it can be used as a functional material in various fields.					
37110788	2	20	theme	oil	283:285	arg1	process					312:318	an oil and hot-water extraction process	280:318	an oil and hot-water extraction process	280:318	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	4	21	theme	-2,5-diphenyl-2H-tetrazolium	668:695	arg1	assay					705:709	a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay	638:709	a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay to evaluate the cell viability of the EUFOC	638:753	We conducted a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay to evaluate the cell viability of the EUFOC, and the results showed that EUFOC was not cytotoxic at the test concentrations.					
37110788	0	22	theme	Fatty	12:16	arg1	Acids					18:22	Unsaturated Fatty Acids	0:22	Unsaturated Fatty Acids Complex	0:30	Unsaturated Fatty Acids Complex Regulates Inflammatory Cytokine Production through the Hyaluronic Acid Pathway.					
37110788	10	23	theme	various	1550:1556	arg1	fields					1558:1563	various fields	1550:1563	various fields	1550:1563	These results suggest that the EUFOC has excellent anti-inflammatory and antioxidant properties, and hence, it can be used as a functional material in various fields.					
37110788	9	24	theme	hyaluronic	1311:1320	arg1	content					1327:1333	hyaluronic acid content	1311:1333	hyaluronic acid content	1311:1333	In addition, hyaluronic acid content was markedly increased by the EUFOC in a dose-dependent manner.					
37110788	3	25	theme	oxide	575:579	arg1	production					581:590	nitric oxide production	568:590	nitric oxide production	568:590	Furthermore, the antioxidant effect of the extract complex was evaluated, and the anti-inflammatory effect was explored by assessing its inhibitory effect on nitric oxide production through its HA-promoting effect.					
37110788	3	26	theme	antioxidant	427:437	arg1	effect					439:444	the antioxidant effect	423:444	the antioxidant effect of the extract complex	423:467	Furthermore, the antioxidant effect of the extract complex was evaluated, and the anti-inflammatory effect was explored by assessing its inhibitory effect on nitric oxide production through its HA-promoting effect.					
37110788	0	27	theme	Unsaturated	0:10	arg1	Acids					18:22	Unsaturated Fatty Acids	0:22	Unsaturated Fatty Acids Complex	0:30	Unsaturated Fatty Acids Complex Regulates Inflammatory Cytokine Production through the Hyaluronic Acid Pathway.					
37110788	5	28	theme	endogenous	862:871	arg1	cytotoxicity					873:884	no endogenous cytotoxicity	859:884	no endogenous cytotoxicity	859:884	In addition, it showed no endogenous cytotoxicity in HaCaT (human keratinocyte) cells.					
37110788	2	29	from	plants	265:270	arg1	extracts					243:250	extracts	243:250	extracts from natural plants	243:270	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	2	30	theme	complex	393:399	arg1	EUFOC					402:406	EUFOC	402:406	EUFOC	402:406	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	2	30	theme	complex	393:399	arg1	acid					388:391	an effective unsaturated fatty acid complex	357:399	an effective unsaturated fatty acid complex (EUFOC)	357:407	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	0	31	theme	Acids	18:22	arg1	Complex					24:30	Unsaturated Fatty Acids Complex	0:30	Unsaturated Fatty Acids Complex	0:30	Unsaturated Fatty Acids Complex Regulates Inflammatory Cytokine Production through the Hyaluronic Acid Pathway.					
37110788	7	32	theme	cell	1116:1119	arg1	viability					1121:1129	cell viability	1116:1129	cell viability	1116:1129	Moreover, it exerted an inhibitory effect on NO production at concentrations that did not inhibit cell viability.					
37110788	3	33	theme	nitric	568:573	arg1	oxide					575:579	nitric oxide	568:579	nitric oxide production	568:590	Furthermore, the antioxidant effect of the extract complex was evaluated, and the anti-inflammatory effect was explored by assessing its inhibitory effect on nitric oxide production through its HA-promoting effect.					
37110788	1	34	theme	anti-inflammatory	204:220	arg1	effects					222:228	antioxidant and anti-inflammatory effects	188:228	antioxidant and anti-inflammatory effects	188:228	In this study, we aimed to develop natural and/or functional materials with antioxidant and anti-inflammatory effects.					
37110788	0	35	theme	Inflammatory	42:53	arg1	Production					64:73	Inflammatory Cytokine Production	42:73	Inflammatory Cytokine Production	42:73	Unsaturated Fatty Acids Complex Regulates Inflammatory Cytokine Production through the Hyaluronic Acid Pathway.					
37110788	6	36	theme	superoxide-scavenging	985:1005	arg1	abilities					1007:1015	excellent 1,1-diphenyl-2-picrylhydrazyl- and superoxide-scavenging abilities	940:1015	excellent 1,1-diphenyl-2-picrylhydrazyl- and superoxide-scavenging abilities	940:1015	The EUFOC showed excellent 1,1-diphenyl-2-picrylhydrazyl- and superoxide-scavenging abilities.					
37110788	3	37	theme	HA-promoting	604:615	arg1	effect					617:622	its HA-promoting effect	600:622	its HA-promoting effect	600:622	Furthermore, the antioxidant effect of the extract complex was evaluated, and the anti-inflammatory effect was explored by assessing its inhibitory effect on nitric oxide production through its HA-promoting effect.					
37110788	1	38	theme	antioxidant	188:198	arg1	effects					222:228	antioxidant and anti-inflammatory effects	188:228	antioxidant and anti-inflammatory effects	188:228	In this study, we aimed to develop natural and/or functional materials with antioxidant and anti-inflammatory effects.					
37110788	3	39	theme	extract	453:459	arg1	complex					461:467	the extract complex	449:467	the extract complex	449:467	Furthermore, the antioxidant effect of the extract complex was evaluated, and the anti-inflammatory effect was explored by assessing its inhibitory effect on nitric oxide production through its HA-promoting effect.					
37110788	4	40	theme	bromide	697:703	arg1	assay					705:709	a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay	638:709	a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay to evaluate the cell viability of the EUFOC	638:753	We conducted a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay to evaluate the cell viability of the EUFOC, and the results showed that EUFOC was not cytotoxic at the test concentrations.					
37110788	2	41	theme	unsaturated	370:380	arg1	EUFOC					402:406	EUFOC	402:406	EUFOC	402:406	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	2	41	theme	unsaturated	370:380	arg1	acid					388:391	an effective unsaturated fatty acid complex	357:399	an effective unsaturated fatty acid complex (EUFOC)	357:407	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	3	42	theme	complex	461:467	arg1	effect					439:444	the antioxidant effect	423:444	the antioxidant effect of the extract complex	423:467	Furthermore, the antioxidant effect of the extract complex was evaluated, and the anti-inflammatory effect was explored by assessing its inhibitory effect on nitric oxide production through its HA-promoting effect.					
37110788	10	43	used	used	1517:1520	arg2	it					1507:1508	it	1507:1508	it	1507:1508	These results suggest that the EUFOC has excellent anti-inflammatory and antioxidant properties, and hence, it can be used as a functional material in various fields.					
37110788	10	43	used	used	1517:1520	arg2	material					1538:1545	a functional material	1525:1545	a functional material in various fields	1525:1563	These results suggest that the EUFOC has excellent anti-inflammatory and antioxidant properties, and hence, it can be used as a functional material in various fields.					
37110788	6	44	theme	1,1-diphenyl-2-picrylhydrazyl-	950:979	arg1	abilities					1007:1015	excellent 1,1-diphenyl-2-picrylhydrazyl- and superoxide-scavenging abilities	940:1015	excellent 1,1-diphenyl-2-picrylhydrazyl- and superoxide-scavenging abilities	940:1015	The EUFOC showed excellent 1,1-diphenyl-2-picrylhydrazyl- and superoxide-scavenging abilities.					
37110788	7	45	theme	NO	1063:1064	arg1	production					1066:1075	NO production	1063:1075	NO production	1063:1075	Moreover, it exerted an inhibitory effect on NO production at concentrations that did not inhibit cell viability.					
37110788	0	46	theme	Cytokine	55:62	arg1	Production					64:73	Inflammatory Cytokine Production	42:73	Inflammatory Cytokine Production	42:73	Unsaturated Fatty Acids Complex Regulates Inflammatory Cytokine Production through the Hyaluronic Acid Pathway.					
37110788	6	47	theme	excellent	940:948	arg1	abilities					1007:1015	excellent 1,1-diphenyl-2-picrylhydrazyl- and superoxide-scavenging abilities	940:1015	excellent 1,1-diphenyl-2-picrylhydrazyl- and superoxide-scavenging abilities	940:1015	The EUFOC showed excellent 1,1-diphenyl-2-picrylhydrazyl- and superoxide-scavenging abilities.					
37110788	7	48	theme	inhibitory	1042:1051	arg1	effect					1053:1058	an inhibitory effect	1039:1058	an inhibitory effect on NO production	1039:1075	Moreover, it exerted an inhibitory effect on NO production at concentrations that did not inhibit cell viability.					
37110788	3	49	theme	anti-inflammatory	492:508	arg1	effect					510:515	the anti-inflammatory effect	488:515	the anti-inflammatory effect	488:515	Furthermore, the antioxidant effect of the extract complex was evaluated, and the anti-inflammatory effect was explored by assessing its inhibitory effect on nitric oxide production through its HA-promoting effect.					
37110788	8	50	theme	cytokines	1157:1165	arg1	secretion					1136:1144	The secretion	1132:1144	The secretion of all the cytokines	1132:1165	The secretion of all the cytokines was increased by lipopolysaccharide (LPS) treatment; however, this was inhibited by the EUFOC in a concentration-dependent manner.					
37110788	4	51	from	concentrations	820:833	arg1	cytotoxic					798:806	cytotoxic	798:806	cytotoxic	798:806	We conducted a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay to evaluate the cell viability of the EUFOC, and the results showed that EUFOC was not cytotoxic at the test concentrations.					
37110788	5	52	theme	HaCaT	889:893	arg1	cells					916:920	HaCaT (human keratinocyte) cells	889:920	HaCaT (human keratinocyte) cells	889:920	In addition, it showed no endogenous cytotoxicity in HaCaT (human keratinocyte) cells.					
37110788	1	53	with	materials	173:181	arg1	effects					222:228	antioxidant and anti-inflammatory effects	188:228	antioxidant and anti-inflammatory effects	188:228	In this study, we aimed to develop natural and/or functional materials with antioxidant and anti-inflammatory effects.					
37110788	3	54	theme	inhibitory	547:556	arg1	effect					558:563	its inhibitory effect	543:563	its inhibitory effect on nitric oxide production	543:590	Furthermore, the antioxidant effect of the extract complex was evaluated, and the anti-inflammatory effect was explored by assessing its inhibitory effect on nitric oxide production through its HA-promoting effect.					
37110788	2	55	theme	extract	336:342	arg1	composite					344:352	an extract composite	333:352	an extract composite of an effective unsaturated fatty acid complex (EUFOC)	333:407	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	2	55	theme	extract	336:342	arg1	acid					388:391	an effective unsaturated fatty acid complex	357:399	an effective unsaturated fatty acid complex (EUFOC)	357:407	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	9	56	theme	dose-dependent	1376:1389	arg1	manner					1391:1396	a dose-dependent manner	1374:1396	a dose-dependent manner	1374:1396	In addition, hyaluronic acid content was markedly increased by the EUFOC in a dose-dependent manner.					
37110788	5	57	theme	human	896:900	arg1	cells					916:920	HaCaT (human keratinocyte) cells	889:920	HaCaT (human keratinocyte) cells	889:920	In addition, it showed no endogenous cytotoxicity in HaCaT (human keratinocyte) cells.					
37110788	4	58	theme	test	815:818	arg1	concentrations					820:833	the test concentrations	811:833	the test concentrations	811:833	We conducted a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay to evaluate the cell viability of the EUFOC, and the results showed that EUFOC was not cytotoxic at the test concentrations.					
37110788	10	59	theme	functional	1527:1536	arg1	material					1538:1545	a functional material	1525:1545	a functional material in various fields	1525:1563	These results suggest that the EUFOC has excellent anti-inflammatory and antioxidant properties, and hence, it can be used as a functional material in various fields.					
37110788	10	59	theme	functional	1527:1536	arg1	it					1507:1508	it	1507:1508	it	1507:1508	These results suggest that the EUFOC has excellent anti-inflammatory and antioxidant properties, and hence, it can be used as a functional material in various fields.					
37110788	2	60	theme	acid	388:391	arg1	composite					344:352	an extract composite	333:352	an extract composite of an effective unsaturated fatty acid complex (EUFOC)	333:407	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	2	60	theme	acid	388:391	arg1	acid					388:391	an effective unsaturated fatty acid complex	357:399	an effective unsaturated fatty acid complex (EUFOC)	357:407	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	2	61	theme	natural	257:263	arg1	plants					265:270	natural plants	257:270	natural plants	257:270	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	2	62	theme	fatty	382:386	arg1	EUFOC					402:406	EUFOC	402:406	EUFOC	402:406	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
37110788	2	62	theme	fatty	382:386	arg1	acid					388:391	an effective unsaturated fatty acid complex	357:399	an effective unsaturated fatty acid complex (EUFOC)	357:407	We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC).					
34878454	0	0	theme	humanized	98:106	arg1	mice					108:111	humanized mice	98:111	humanized mice	98:111	Saccharomyces boulardii alleviates DSS-induced intestinal barrier dysfunction and inflammation in humanized mice.					
34878454	7	1	theme	microbial	1001:1009	arg1	levels					1028:1033	the microbial metabolite SCFAs levels	997:1033	the microbial metabolite SCFAs levels	997:1033	Further analysis demonstrated that S. boulardii-derived polysaccharides and polypeptides promoted the growth of certain probiotics and increased the microbial metabolite SCFAs levels.					
34878454	1	2	theme	boulardii	193:201	arg1	effect					169:174	a beneficial effect	156:174	a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD)	156:252	Recent clinical studies have demonstrated a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD).					
34878454	4	3	dep	boulardii-supplement	504:523	arg1	S.					501:502	S.	501:502	S.	501:502	The mice were fed an S. boulardii-supplement diet for 16 days before DSS treatment.					
34878454	6	4	theme	acid	828:831	arg1	metabolism					840:849	short-chain fatty acid (SCFA) metabolism	810:849	short-chain fatty acid (SCFA) metabolism	810:849	These changes were found to be associated with an altered microbiome composition and short-chain fatty acid (SCFA) metabolism.					
34878454	7	5	theme	boulardii-derived	890:906	arg1	polysaccharides					908:922	polysaccharides	908:922	polysaccharides	908:922	Further analysis demonstrated that S. boulardii-derived polysaccharides and polypeptides promoted the growth of certain probiotics and increased the microbial metabolite SCFAs levels.					
34878454	5	6	theme	inflammatory	666:677	arg1	responses					679:687	inflammatory responses	666:687	inflammatory responses	666:687	The results showed that feeding S. boulardii significantly ameliorated the colon damage and regulated inflammatory responses by modulating the cytokine profile.					
34878454	1	7	from	effect	169:174	arg1	IBD					249:251	IBD	249:251	IBD	249:251	Recent clinical studies have demonstrated a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD).					
34878454	1	7	from	effect	169:174	arg1	disease					240:246	inflammatory bowel disease	221:246	inflammatory bowel disease (IBD)	221:252	Recent clinical studies have demonstrated a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD).					
34878454	3	8	theme	modulating	348:357	arg1	effect					359:364	the modulating effect	344:364	the modulating effect of S. boulardii on the intestinal microbiota	344:409	In this study, we investigated the modulating effect of S. boulardii on the intestinal microbiota in humanized mice with dextran sulfate sodium (DSS)-induced colitis.					
34878454	0	9	from	dysfunction	66:76	arg1	mice					108:111	humanized mice	98:111	humanized mice	98:111	Saccharomyces boulardii alleviates DSS-induced intestinal barrier dysfunction and inflammation in humanized mice.					
34878454	1	10	theme	inflammatory	221:232	arg1	IBD					249:251	IBD	249:251	IBD	249:251	Recent clinical studies have demonstrated a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD).					
34878454	1	10	theme	inflammatory	221:232	arg1	disease					240:246	inflammatory bowel disease	221:246	inflammatory bowel disease (IBD)	221:252	Recent clinical studies have demonstrated a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD).					
34878454	3	11	theme	humanized	414:422	arg1	mice					424:427	humanized mice	414:427	humanized mice with dextran sulfate sodium (DSS)-induced colitis	414:477	In this study, we investigated the modulating effect of S. boulardii on the intestinal microbiota in humanized mice with dextran sulfate sodium (DSS)-induced colitis.					
34878454	7	12	theme	probiotics	972:981	arg1	growth					954:959	the growth	950:959	the growth of certain probiotics	950:981	Further analysis demonstrated that S. boulardii-derived polysaccharides and polypeptides promoted the growth of certain probiotics and increased the microbial metabolite SCFAs levels.					
34878454	3	13	with	mice	424:427	arg1	colitis					471:477	dextran sulfate sodium (DSS)-induced colitis	434:477	dextran sulfate sodium (DSS)-induced colitis	434:477	In this study, we investigated the modulating effect of S. boulardii on the intestinal microbiota in humanized mice with dextran sulfate sodium (DSS)-induced colitis.					
34878454	7	14	theme	Further	852:858	arg1	analysis					860:867	Further analysis	852:867	Further analysis	852:867	Further analysis demonstrated that S. boulardii-derived polysaccharides and polypeptides promoted the growth of certain probiotics and increased the microbial metabolite SCFAs levels.					
34878454	8	15	theme	potential	1103:1111	arg1	modulator					1128:1136	a potential gut microbiota modulator	1101:1136	a potential gut microbiota modulator to prevent and treat IBD	1101:1161	Overall, these findings demonstrated the role of S. boulardii as a potential gut microbiota modulator to prevent and treat IBD.					
34878454	3	16	theme	intestinal	389:398	arg1	microbiota					400:409	the intestinal microbiota	385:409	the intestinal microbiota	385:409	In this study, we investigated the modulating effect of S. boulardii on the intestinal microbiota in humanized mice with dextran sulfate sodium (DSS)-induced colitis.					
34878454	5	17	theme	colon	639:643	arg1	damage					645:650	the colon damage	635:650	the colon damage	635:650	The results showed that feeding S. boulardii significantly ameliorated the colon damage and regulated inflammatory responses by modulating the cytokine profile.					
34878454	6	18	theme	microbiome	783:792	arg1	composition					794:804	an altered microbiome composition	772:804	an altered microbiome composition	772:804	These changes were found to be associated with an altered microbiome composition and short-chain fatty acid (SCFA) metabolism.					
34878454	1	19	theme	Recent	114:119	arg1	studies					130:136	Recent clinical studies	114:136	Recent clinical studies	114:136	Recent clinical studies have demonstrated a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD).					
34878454	7	20	link	boulardii-derived	890:906	arg1	polysaccharides					908:922	polysaccharides	908:922	polysaccharides	908:922	Further analysis demonstrated that S. boulardii-derived polysaccharides and polypeptides promoted the growth of certain probiotics and increased the microbial metabolite SCFAs levels.					
34878454	3	21	theme	boulardii	372:380	arg1	effect					359:364	the modulating effect	344:364	the modulating effect of S. boulardii on the intestinal microbiota	344:409	In this study, we investigated the modulating effect of S. boulardii on the intestinal microbiota in humanized mice with dextran sulfate sodium (DSS)-induced colitis.					
34878454	7	22	dep	polysaccharides	908:922	arg1	S.					887:888	S. boulardii-derived polysaccharides and polypeptides	887:939	S. boulardii-derived polysaccharides and polypeptides	887:939	Further analysis demonstrated that S. boulardii-derived polysaccharides and polypeptides promoted the growth of certain probiotics and increased the microbial metabolite SCFAs levels.					
34878454	5	23	theme	cytokine	707:714	arg1	profile					716:722	the cytokine profile	703:722	the cytokine profile	703:722	The results showed that feeding S. boulardii significantly ameliorated the colon damage and regulated inflammatory responses by modulating the cytokine profile.					
34878454	6	24	theme	altered	775:781	arg1	composition					794:804	an altered microbiome composition	772:804	an altered microbiome composition	772:804	These changes were found to be associated with an altered microbiome composition and short-chain fatty acid (SCFA) metabolism.					
34878454	1	25	theme	clinical	121:128	arg1	studies					130:136	Recent clinical studies	114:136	Recent clinical studies	114:136	Recent clinical studies have demonstrated a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD).					
34878454	1	26	dep	boulardii	193:201	arg1	boulardii					207:215	S. boulardii	204:215	S. boulardii	204:215	Recent clinical studies have demonstrated a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD).					
34878454	8	27	theme	gut	1113:1115	arg1	modulator					1128:1136	a potential gut microbiota modulator	1101:1136	a potential gut microbiota modulator to prevent and treat IBD	1101:1161	Overall, these findings demonstrated the role of S. boulardii as a potential gut microbiota modulator to prevent and treat IBD.					
34878454	3	28	theme	-induced	462:469	arg1	colitis					471:477	dextran sulfate sodium (DSS)-induced colitis	434:477	dextran sulfate sodium (DSS)-induced colitis	434:477	In this study, we investigated the modulating effect of S. boulardii on the intestinal microbiota in humanized mice with dextran sulfate sodium (DSS)-induced colitis.					
34878454	0	29	theme	barrier	58:64	arg1	dysfunction					66:76	DSS-induced intestinal barrier dysfunction	35:76	DSS-induced intestinal barrier dysfunction	35:76	Saccharomyces boulardii alleviates DSS-induced intestinal barrier dysfunction and inflammation in humanized mice.					
34878454	4	30	theme	DSS	549:551	arg1	treatment					553:561	DSS treatment	549:561	DSS treatment	549:561	The mice were fed an S. boulardii-supplement diet for 16 days before DSS treatment.					
34878454	3	31	from	effect	359:364	arg1	microbiota					400:409	the intestinal microbiota	385:409	the intestinal microbiota	385:409	In this study, we investigated the modulating effect of S. boulardii on the intestinal microbiota in humanized mice with dextran sulfate sodium (DSS)-induced colitis.					
34878454	1	32	theme	bowel	234:238	arg1	IBD					249:251	IBD	249:251	IBD	249:251	Recent clinical studies have demonstrated a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD).					
34878454	1	32	theme	bowel	234:238	arg1	disease					240:246	inflammatory bowel disease	221:246	inflammatory bowel disease (IBD)	221:252	Recent clinical studies have demonstrated a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD).					
34878454	0	33	theme	intestinal	47:56	arg1	dysfunction					66:76	DSS-induced intestinal barrier dysfunction	35:76	DSS-induced intestinal barrier dysfunction	35:76	Saccharomyces boulardii alleviates DSS-induced intestinal barrier dysfunction and inflammation in humanized mice.					
34878454	0	34	theme	DSS-induced	35:45	arg1	dysfunction					66:76	DSS-induced intestinal barrier dysfunction	35:76	DSS-induced intestinal barrier dysfunction	35:76	Saccharomyces boulardii alleviates DSS-induced intestinal barrier dysfunction and inflammation in humanized mice.					
34878454	4	35	theme	boulardii-supplement	504:523	arg1	diet					525:528	an S. boulardii-supplement diet	498:528	an S. boulardii-supplement diet	498:528	The mice were fed an S. boulardii-supplement diet for 16 days before DSS treatment.					
34878454	6	36	theme	fatty	822:826	arg1	SCFA					834:837	SCFA	834:837	SCFA	834:837	These changes were found to be associated with an altered microbiome composition and short-chain fatty acid (SCFA) metabolism.					
34878454	6	36	theme	fatty	822:826	arg1	acid					828:831	short-chain fatty acid	810:831	short-chain fatty acid (SCFA) metabolism	810:849	These changes were found to be associated with an altered microbiome composition and short-chain fatty acid (SCFA) metabolism.					
34878454	0	37	from	inflammation	82:93	arg1	mice					108:111	humanized mice	98:111	humanized mice	98:111	Saccharomyces boulardii alleviates DSS-induced intestinal barrier dysfunction and inflammation in humanized mice.					
34878454	8	38	theme	microbiota	1117:1126	arg1	modulator					1128:1136	a potential gut microbiota modulator	1101:1136	a potential gut microbiota modulator to prevent and treat IBD	1101:1161	Overall, these findings demonstrated the role of S. boulardii as a potential gut microbiota modulator to prevent and treat IBD.					
34878454	8	39	theme	boulardii	1088:1096	arg1	role					1077:1080	the role	1073:1080	the role of S. boulardii as a potential gut microbiota modulator to prevent and treat IBD	1073:1161	Overall, these findings demonstrated the role of S. boulardii as a potential gut microbiota modulator to prevent and treat IBD.					
34878454	7	40	theme	certain	964:970	arg1	probiotics					972:981	certain probiotics	964:981	certain probiotics	964:981	Further analysis demonstrated that S. boulardii-derived polysaccharides and polypeptides promoted the growth of certain probiotics and increased the microbial metabolite SCFAs levels.					
34878454	2	41	theme	underlying	268:277	arg1	mechanisms					279:288	the underlying mechanisms	264:288	the underlying mechanisms	264:288	However, the underlying mechanisms remain poorly defined.					
34878454	7	42	theme	metabolite	1011:1020	arg1	levels					1028:1033	the microbial metabolite SCFAs levels	997:1033	the microbial metabolite SCFAs levels	997:1033	Further analysis demonstrated that S. boulardii-derived polysaccharides and polypeptides promoted the growth of certain probiotics and increased the microbial metabolite SCFAs levels.					
34878454	1	43	theme	beneficial	158:167	arg1	effect					169:174	a beneficial effect	156:174	a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD)	156:252	Recent clinical studies have demonstrated a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD).					
34878454	6	44	theme	short-chain	810:820	arg1	SCFA					834:837	SCFA	834:837	SCFA	834:837	These changes were found to be associated with an altered microbiome composition and short-chain fatty acid (SCFA) metabolism.					
34878454	6	44	theme	short-chain	810:820	arg1	acid					828:831	short-chain fatty acid	810:831	short-chain fatty acid (SCFA) metabolism	810:849	These changes were found to be associated with an altered microbiome composition and short-chain fatty acid (SCFA) metabolism.					
34878454	7	45	theme	SCFAs	1022:1026	arg1	levels					1028:1033	the microbial metabolite SCFAs levels	997:1033	the microbial metabolite SCFAs levels	997:1033	Further analysis demonstrated that S. boulardii-derived polysaccharides and polypeptides promoted the growth of certain probiotics and increased the microbial metabolite SCFAs levels.					
35522531	4	0	theme	Matrigel-free	864:876	arg1	conditions					878:887	Matrigel-free conditions	864:887	Matrigel-free conditions	864:887	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	5	1	theme	cells	1129:1133	arg1	fate					1091:1094	the alveolar type 2 cell fate	1066:1094	the alveolar type 2 cell fate of human iAT2-derived progenitor cells	1066:1133	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	4	2	theme	chemical	698:705	arg1	properties					720:729	defined chemical and physical properties	690:729	defined chemical and physical properties	690:729	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	6	3	theme	epithelial	1332:1341	arg1	progenitor					1343:1352	other epithelial progenitor and stem cell aggregates	1326:1377	progenitor	1343:1352	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	4	4	theme	defined	690:696	arg1	properties					720:729	defined chemical and physical properties	690:729	defined chemical and physical properties	690:729	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	6	5	theme	primary	1289:1295	arg1	tissue					1303:1308	primary mouse tissue	1289:1308	primary mouse tissue derived AT2	1289:1320	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	5	6	gly	heterogeneity	958:970	arg1	structure					998:1006	structure	998:1006	structure	998:1006	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	5	6	gly	heterogeneity	958:970	arg1	size					989:992	alveolosphere size	975:992	alveolosphere size	975:992	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	4	7	theme	alveolar	783:790	arg1	iAT2s					806:810	iAT2s	806:810	iAT2s	806:810	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	4	7	theme	alveolar	783:790	arg1	cells					799:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells	732:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s)	732:811	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	6	8	theme	accessible	1173:1182	arg1	system					1184:1189	a facile and accessible system	1160:1189	a facile and accessible system for the culture of iPSC-derived lung progenitors	1160:1238	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	6	8	theme	accessible	1173:1182	arg1	system					1150:1155	This hydrogel system	1136:1155	This hydrogel system	1136:1155	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	4	9	theme	iPSC	769:772	arg1	iAT2s					806:810	iAT2s	806:810	iAT2s	806:810	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	4	9	theme	iPSC	769:772	arg1	cells					799:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells	732:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s)	732:811	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	5	10	theme	alveolosphere	975:987	arg1	size					989:992	alveolosphere size	975:992	alveolosphere size	975:992	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	3	11	theme	hyaluronic	555:564	arg1	hydrogels					571:579	hyaluronic acid hydrogels	555:579	hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres)	555:656	Here, a method is described that relies on hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres).					
35522531	5	12	theme	human	1099:1103	arg1	cells					1129:1133	human iAT2-derived progenitor cells	1099:1133	human iAT2-derived progenitor cells	1099:1133	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	2	13	dep	shape	485:489	arg1	i.e.					479:482	i.e.	479:482	i.e.	479:482	Despite their potential, current protocols to grow these organoids almost exclusively depend on culture within 3D Matrigel, which limits defined culture conditions, introduces animal components, and results in heterogenous organoids (i.e., shape, size, composition).					
35522531	6	14	theme	iPSC-derived	1210:1221	arg1	progenitors					1228:1238	iPSC-derived lung progenitors	1210:1238	iPSC-derived lung progenitors	1210:1238	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	5	15	theme	predefined	905:914	arg1	microcavities					916:928	predefined microcavities	905:928	predefined microcavities within these hydrogels	905:951	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	6	16	theme	stem	1358:1361	arg1	aggregates					1368:1377	other epithelial progenitor and stem cell aggregates	1326:1377	aggregates	1368:1377	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	4	17	theme	physical	711:718	arg1	properties					720:729	defined chemical and physical properties	690:729	defined chemical and physical properties	690:729	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	4	18	theme	pluripotent	746:756	arg1	iAT2s					806:810	iAT2s	806:810	iAT2s	806:810	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	4	18	theme	pluripotent	746:756	arg1	cells					799:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells	732:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s)	732:811	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	4	19	theme	-derived	774:781	arg1	iAT2s					806:810	iAT2s	806:810	iAT2s	806:810	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	4	19	theme	-derived	774:781	arg1	cells					799:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells	732:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s)	732:811	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	2	20	theme	animal	421:426	arg1	components					428:437	animal components	421:437	animal components	421:437	Despite their potential, current protocols to grow these organoids almost exclusively depend on culture within 3D Matrigel, which limits defined culture conditions, introduces animal components, and results in heterogenous organoids (i.e., shape, size, composition).					
35522531	5	21	theme	progenitor	1118:1127	arg1	cells					1129:1133	human iAT2-derived progenitor cells	1099:1133	human iAT2-derived progenitor cells	1099:1133	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	1	22	theme	Epithelial	86:95	arg1	organoids					102:110	Epithelial cell organoids	86:110	Epithelial cell organoids	86:110	Epithelial cell organoids have increased opportunities to probe questions on tissue development and disease in vitro and for therapeutic cell transplantation.					
35522531	5	23	theme	iAT2-derived	1105:1116	arg1	cells					1129:1133	human iAT2-derived progenitor cells	1099:1133	human iAT2-derived progenitor cells	1099:1133	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	4	24	with	hydrogels	675:683	arg1	properties					720:729	defined chemical and physical properties	690:729	defined chemical and physical properties	690:729	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	1	25	from	questions	150:158	arg1	disease					186:192	disease	186:192	disease	186:192	Epithelial cell organoids have increased opportunities to probe questions on tissue development and disease in vitro and for therapeutic cell transplantation.					
35522531	1	25	from	questions	150:158	arg1	development					170:180	tissue development	163:180	tissue development	163:180	Epithelial cell organoids have increased opportunities to probe questions on tissue development and disease in vitro and for therapeutic cell transplantation.					
35522531	1	26	theme	cell	97:100	arg1	organoids					102:110	Epithelial cell organoids	86:110	Epithelial cell organoids	86:110	Epithelial cell organoids have increased opportunities to probe questions on tissue development and disease in vitro and for therapeutic cell transplantation.					
35522531	5	27	link	iAT2-derived	1105:1116	arg1	cells					1129:1133	human iAT2-derived progenitor cells	1099:1133	human iAT2-derived progenitor cells	1099:1133	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	4	28	theme	synthetic	665:673	arg1	hydrogels					675:683	synthetic hydrogels	665:683	synthetic hydrogels with defined chemical and physical properties	665:729	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	0	29	theme	Microstructured	0:14	arg1	Hydrogels					16:24	Microstructured Hydrogels	0:24	Microstructured Hydrogels	0:24	Microstructured Hydrogels to Guide Self-Assembly and Function of Lung Alveolospheres.					
35522531	3	30	theme	acid	566:569	arg1	hydrogels					571:579	hyaluronic acid hydrogels	555:579	hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres)	555:656	Here, a method is described that relies on hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres).					
35522531	3	31	dep	generation	589:598	arg1	the					585:587	the	585:587	the	585:587	Here, a method is described that relies on hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres).					
35522531	2	32	theme	heterogenous	455:466	arg1	organoids					468:476	heterogenous organoids	455:476	heterogenous organoids (i.e., shape, size, composition)	455:509	Despite their potential, current protocols to grow these organoids almost exclusively depend on culture within 3D Matrigel, which limits defined culture conditions, introduces animal components, and results in heterogenous organoids (i.e., shape, size, composition).					
35522531	6	33	theme	progenitor	1343:1352	arg1	culture					1278:1284	the culture	1274:1284	the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates	1274:1377	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	6	34	theme	progenitors	1228:1238	arg1	culture					1199:1205	the culture	1195:1205	the culture of iPSC-derived lung progenitors	1195:1238	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	4	35	theme	cell	763:766	arg1	iAT2s					806:810	iAT2s	806:810	iAT2s	806:810	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	4	35	theme	cell	763:766	arg1	cells					799:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells	732:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s)	732:811	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	5	36	theme	size	989:992	arg1	heterogeneity					958:970	the heterogeneity	954:970	the heterogeneity of alveolosphere size and structure	954:1006	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	4	37	theme	human-induced	732:744	arg1	iAT2s					806:810	iAT2s	806:810	iAT2s	806:810	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	4	37	theme	human-induced	732:744	arg1	cells					799:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells	732:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s)	732:811	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	5	38	theme	structure	998:1006	arg1	heterogeneity					958:970	the heterogeneity	954:970	the heterogeneity of alveolosphere size and structure	954:1006	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	4	39	theme	stem	758:761	arg1	iAT2s					806:810	iAT2s	806:810	iAT2s	806:810	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	4	39	theme	stem	758:761	arg1	cells					799:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells	732:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s)	732:811	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	1	40	theme	therapeutic	211:221	arg1	transplantation					228:242	therapeutic cell transplantation	211:242	therapeutic cell transplantation	211:242	Epithelial cell organoids have increased opportunities to probe questions on tissue development and disease in vitro and for therapeutic cell transplantation.					
35522531	6	41	theme	other	1326:1330	arg1	progenitor					1343:1352	other epithelial progenitor and stem cell aggregates	1326:1377	progenitor	1343:1352	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	5	42	theme	cell	1086:1089	arg1	fate					1091:1094	the alveolar type 2 cell fate	1066:1094	the alveolar type 2 cell fate of human iAT2-derived progenitor cells	1066:1133	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	5	43	theme	3D	1036:1037	arg1	culture					1039:1045	3D culture	1036:1045	3D culture	1036:1045	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	1	44	theme	cell	223:226	arg1	transplantation					228:242	therapeutic cell transplantation	211:242	therapeutic cell transplantation	211:242	Epithelial cell organoids have increased opportunities to probe questions on tissue development and disease in vitro and for therapeutic cell transplantation.					
35522531	3	45	theme	alveolar	622:629	arg1	alveolospheres					642:655	alveolospheres	642:655	alveolospheres	642:655	Here, a method is described that relies on hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres).					
35522531	3	45	theme	alveolar	622:629	arg1	organoids					631:639	lung alveolar organoids	617:639	lung alveolar organoids (alveolospheres)	617:656	Here, a method is described that relies on hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres).					
35522531	3	46	theme	organoids	631:639	arg1	expansion					604:612	expansion	604:612	expansion	604:612	Here, a method is described that relies on hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres).					
35522531	3	46	theme	organoids	631:639	arg1	generation					589:598	generation	589:598	generation	589:598	Here, a method is described that relies on hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres).					
35522531	4	47	link	-derived	774:781	arg1	iAT2s					806:810	iAT2s	806:810	iAT2s	806:810	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	4	47	link	-derived	774:781	arg1	cells					799:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells	732:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s)	732:811	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	2	48	theme	defined	382:388	arg1	conditions					398:407	defined culture conditions	382:407	defined culture conditions	382:407	Despite their potential, current protocols to grow these organoids almost exclusively depend on culture within 3D Matrigel, which limits defined culture conditions, introduces animal components, and results in heterogenous organoids (i.e., shape, size, composition).					
35522531	1	49	theme	tissue	163:168	arg1	development					170:180	tissue development	163:180	tissue development	163:180	Epithelial cell organoids have increased opportunities to probe questions on tissue development and disease in vitro and for therapeutic cell transplantation.					
35522531	6	50	theme	hydrogel	1141:1148	arg1	system					1184:1189	a facile and accessible system	1160:1189	a facile and accessible system for the culture of iPSC-derived lung progenitors	1160:1238	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	6	50	theme	hydrogel	1141:1148	arg1	system					1150:1155	This hydrogel system	1136:1155	This hydrogel system	1136:1155	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	6	51	link	iPSC-derived	1210:1221	arg1	progenitors					1228:1238	iPSC-derived lung progenitors	1210:1238	iPSC-derived lung progenitors	1210:1238	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	6	52	theme	tissue	1303:1308	arg1	culture					1278:1284	the culture	1274:1284	the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates	1274:1377	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	6	53	theme	lung	1223:1226	arg1	progenitors					1228:1238	iPSC-derived lung progenitors	1210:1238	iPSC-derived lung progenitors	1210:1238	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	4	54	theme	type	792:795	arg1	iAT2s					806:810	iAT2s	806:810	iAT2s	806:810	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	4	54	theme	type	792:795	arg1	cells					799:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells	732:803	human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s)	732:811	Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions.					
35522531	2	55	theme	3D	356:357	arg1	Matrigel					359:366	3D Matrigel	356:366	3D Matrigel	356:366	Despite their potential, current protocols to grow these organoids almost exclusively depend on culture within 3D Matrigel, which limits defined culture conditions, introduces animal components, and results in heterogenous organoids (i.e., shape, size, composition).					
35522531	6	56	theme	mouse	1297:1301	arg1	tissue					1303:1308	primary mouse tissue	1289:1308	primary mouse tissue derived AT2	1289:1320	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	5	57	theme	alveolar	1070:1077	arg1	fate					1091:1094	the alveolar type 2 cell fate	1066:1094	the alveolar type 2 cell fate of human iAT2-derived progenitor cells	1066:1133	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	3	58	theme	lung	617:620	arg1	alveolospheres					642:655	alveolospheres	642:655	alveolospheres	642:655	Here, a method is described that relies on hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres).					
35522531	3	58	theme	lung	617:620	arg1	organoids					631:639	lung alveolar organoids	617:639	lung alveolar organoids (alveolospheres)	617:656	Here, a method is described that relies on hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres).					
35522531	2	59	dep	grow	291:294	arg1	depend					331:336	depend	331:336	depend on culture within 3D Matrigel, which limits defined culture conditions, introduces animal components, and results in heterogenous organoids (i.e., shape, size, composition)	331:509	Despite their potential, current protocols to grow these organoids almost exclusively depend on culture within 3D Matrigel, which limits defined culture conditions, introduces animal components, and results in heterogenous organoids (i.e., shape, size, composition).					
35522531	0	60	theme	Alveolospheres	70:83	arg1	Function					53:60	Function	53:60	Function	53:60	Microstructured Hydrogels to Guide Self-Assembly and Function of Lung Alveolospheres.					
35522531	0	60	theme	Alveolospheres	70:83	arg1	Self-Assembly					35:47	Self-Assembly	35:47	Self-Assembly	35:47	Microstructured Hydrogels to Guide Self-Assembly and Function of Lung Alveolospheres.					
35522531	5	61	theme	type	1079:1082	arg1	fate					1091:1094	the alveolar type 2 cell fate	1066:1094	the alveolar type 2 cell fate of human iAT2-derived progenitor cells	1066:1133	By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells.					
35522531	2	62	theme	current	270:276	arg1	protocols					278:286	current protocols	270:286	current protocols	270:286	Despite their potential, current protocols to grow these organoids almost exclusively depend on culture within 3D Matrigel, which limits defined culture conditions, introduces animal components, and results in heterogenous organoids (i.e., shape, size, composition).					
35522531	2	63	theme	culture	390:396	arg1	conditions					398:407	defined culture conditions	382:407	defined culture conditions	382:407	Despite their potential, current protocols to grow these organoids almost exclusively depend on culture within 3D Matrigel, which limits defined culture conditions, introduces animal components, and results in heterogenous organoids (i.e., shape, size, composition).					
35522531	6	64	theme	facile	1162:1167	arg1	system					1184:1189	a facile and accessible system	1160:1189	a facile and accessible system for the culture of iPSC-derived lung progenitors	1160:1238	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	6	64	theme	facile	1162:1167	arg1	system					1150:1155	This hydrogel system	1136:1155	This hydrogel system	1136:1155	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
35522531	0	65	theme	Lung	65:68	arg1	Alveolospheres					70:83	Lung Alveolospheres	65:83	Lung Alveolospheres	65:83	Microstructured Hydrogels to Guide Self-Assembly and Function of Lung Alveolospheres.					
35522531	1	66	dep	increased	117:125	arg1	probe					144:148	probe	144:148	to probe questions on tissue development and disease in vitro	141:201	Epithelial cell organoids have increased opportunities to probe questions on tissue development and disease in vitro and for therapeutic cell transplantation.					
35522531	1	66	dep	increased	117:125	arg1	transplantation					228:242	therapeutic cell transplantation	211:242	therapeutic cell transplantation	211:242	Epithelial cell organoids have increased opportunities to probe questions on tissue development and disease in vitro and for therapeutic cell transplantation.					
35522531	6	67	theme	cell	1363:1366	arg1	aggregates					1368:1377	other epithelial progenitor and stem cell aggregates	1326:1377	aggregates	1368:1377	This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.					
36087866	11	0	theme	first	2339:2343	arg1	time					2345:2348	the first time	2335:2348	the first time	2335:2348	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	8	1	theme	physico-chemical	1542:1557	arg1	factors					1559:1565	physico-chemical factors	1542:1565	physico-chemical factors in terms of proliferation, cell aggregates size and morphology	1542:1628	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	12	2	theme	relevant	2712:2719	arg1	microenvironment					2745:2760	relevant tumour physico-chemical microenvironment	2712:2760	relevant tumour physico-chemical microenvironment	2712:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	11	3	theme	fluid	2248:2252	arg1	flow					2254:2257	fluid flow	2248:2257	fluid flow	2248:2257	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	11	4	theme	in	2187:2188	arg1	models					2196:2201	3D in vitro models	2184:2201	3D in vitro models with varying stiffness, extracellular pH and fluid flow	2184:2257	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	11	5	theme	aggregate	2433:2441	arg1	size					2443:2446	cell aggregate size	2428:2446	cell aggregate size	2428:2446	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	9	6	theme	marker	1818:1823	arg1	expression					1825:1834	mesenchymal cancer stem cell (M-CSCs) marker expression	1780:1834	mesenchymal cancer stem cell (M-CSCs) marker expression	1780:1834	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	1	7	theme	interstitial	273:284	arg1	flow					292:295	interstitial fluid flow	273:295	interstitial fluid flow	273:295	Several physico-chemical properties of the tumour microenvironment (TME) are dysregulated during tumour progression, such as tissue stiffness, extracellular pH and interstitial fluid flow.					
36087866	5	8	theme	physico-chemical	928:943	arg1	composition					980:990	composition	980:990	composition	980:990	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	8	theme	physico-chemical	928:943	arg1	properties					945:954	controlled physico-chemical properties	917:954	controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH)	917:1006	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	8	theme	physico-chemical	928:943	arg1	hydrogel					957:964	hydrogel stiffness and composition	957:990	hydrogel	957:964	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	10	9	theme	same	2158:2161	arg1	time					2163:2166	the same time	2154:2166	the same time	2154:2166	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	9	10	theme	cancer	1792:1797	arg1	cell					1804:1807	mesenchymal cancer stem cell	1780:1807	mesenchymal cancer stem cell (M-CSCs) marker expression	1780:1834	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	9	10	theme	cancer	1792:1797	arg1	M-CSCs					1810:1815	M-CSCs	1810:1815	M-CSCs	1810:1815	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	3	11	theme	dynamic	585:591	arg1	population					593:602	a dynamic population	583:602	a dynamic population within the tumour	583:620	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36087866	3	11	theme	dynamic	585:591	arg1	cells					567:571	Breast cancer stem cells	548:571	Breast cancer stem cells (B-CSCs)	548:580	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36087866	5	12	theme	in	896:897	arg1	models					905:910	3D in vitro models	893:910	3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH)	893:1006	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	9	13	theme	cell	1804:1807	arg1	expression					1825:1834	mesenchymal cancer stem cell (M-CSCs) marker expression	1780:1834	mesenchymal cancer stem cell (M-CSCs) marker expression	1780:1834	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	10	14	theme	microenvironment	2050:2065	arg1	properties					2025:2034	physico-chemical properties	2008:2034	physico-chemical properties of the tumour microenvironment	2008:2065	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	2	15	theme	preclinical	310:320	arg1	models					322:327	Traditional preclinical models	298:327	Traditional preclinical models	298:327	Traditional preclinical models, although useful to study biological processes, do not provide sufficient control over these physico-chemical properties, hence limiting the understanding of cause-effect relationships between the TME and cancer cells.					
36087866	12	16	theme	disease	2541:2547	arg1	phenotypes					2549:2558	disease phenotypes	2541:2558	disease phenotypes	2541:2558	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	11	17	theme	multiple	2373:2380	arg1	phenotypes					2396:2405	multiple breast cancer phenotypes	2373:2405	multiple breast cancer phenotypes	2373:2405	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	10	18	theme	physico-chemical	2008:2023	arg1	properties					2025:2034	physico-chemical properties	2008:2034	physico-chemical properties of the tumour microenvironment	2008:2065	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	4	19	with	essential	762:770	arg1	importance					722:731	their emerging importance	707:731	their emerging importance in disease physiology	707:753	With their emerging importance in disease physiology, it is essential to study the interplay between above-mentioned TME physico-chemical variables and B-CSC marker expression.					
36087866	8	20	contain	have	1504:1507	arg1	lines					1498:1502	both cell lines	1488:1502	both cell lines	1488:1502	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	8	20	contain	have	1504:1507	arg2	responses					1518:1526	distinct responses	1509:1526	distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology	1509:1628	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	11	21	with	models	2196:2201	arg1	stiffness					2216:2224	varying stiffness	2208:2224	varying stiffness	2208:2224	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	11	21	with	models	2196:2201	arg1	pH					2241:2242	extracellular pH	2227:2242	extracellular pH	2227:2242	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	11	21	with	models	2196:2201	arg1	flow					2254:2257	fluid flow	2248:2257	fluid flow	2248:2257	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	8	22	from	factors	1559:1565	arg1	terms					1570:1574	terms	1570:1574	terms of proliferation, cell aggregates size and morphology	1570:1628	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	12	23	theme	new	2518:2520	arg1	ways					2522:2525	new ways	2518:2525	new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment	2518:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	10	24	theme	tumour	2043:2048	arg1	microenvironment					2050:2065	the tumour microenvironment	2039:2065	the tumour microenvironment	2039:2065	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	10	25	from	expression	2109:2118	arg1	model					2141:2145	a single in vitro model	2123:2145	a single in vitro model	2123:2145	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	6	26	theme	adhesion	1285:1292	arg1	stiffness					1251:1259	stiffness	1251:1259	stiffness (2-10 kPa)	1251:1270	Cells encapsulated in alginate-gelatin hydrogels varying in stiffness (2-10 kPa), density and adhesion ligand (gelatin) were perfused (500 µL/min) for up to 14 days.					
36087866	6	26	theme	adhesion	1285:1292	arg1	gelatin					1302:1308	gelatin	1302:1308	gelatin	1302:1308	Cells encapsulated in alginate-gelatin hydrogels varying in stiffness (2-10 kPa), density and adhesion ligand (gelatin) were perfused (500 µL/min) for up to 14 days.					
36087866	6	26	theme	adhesion	1285:1292	arg1	ligand					1294:1299	adhesion ligand	1285:1299	adhesion ligand (gelatin)	1285:1309	Cells encapsulated in alginate-gelatin hydrogels varying in stiffness (2-10 kPa), density and adhesion ligand (gelatin) were perfused (500 µL/min) for up to 14 days.					
36087866	7	27	dep	tumorigenic	1384:1394	arg1	pH					1397:1398	pH 6.5	1397:1402	pH 6.5	1397:1402	Physiological (pH 7.4) and tumorigenic (pH 6.5) media were used to mimic changes in extracellular pH within the TME.					
36087866	10	28	dep	in	2132:2133	arg1	vitro					2135:2139	vitro	2135:2139	vitro	2135:2139	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	2	29	theme	cancer	534:539	arg1	cells					541:545	the TME and cancer cells	522:545	cells	541:545	Traditional preclinical models, although useful to study biological processes, do not provide sufficient control over these physico-chemical properties, hence limiting the understanding of cause-effect relationships between the TME and cancer cells.					
36087866	11	30	theme	cancer	2389:2394	arg1	phenotypes					2396:2405	multiple breast cancer phenotypes	2373:2405	multiple breast cancer phenotypes	2373:2405	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	9	31	theme	direct	1848:1853	arg1	impact					1855:1860	direct impact	1848:1860	direct impact of the physico-chemical microenvironment on disease onset and progression	1848:1934	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	5	32	from	changes	1068:1074	arg1	population					1115:1124	B-CSC population	1109:1124	B-CSC population	1109:1124	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	32	from	changes	1068:1074	arg1	proliferation					1079:1091	proliferation	1079:1091	proliferation	1079:1091	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	32	from	changes	1068:1074	arg1	morphology					1094:1103	morphology	1094:1103	morphology	1094:1103	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	7	33	dep	Physiological	1357:1369	arg1	pH					1372:1373	pH 7.4	1372:1377	pH 7.4	1372:1377	Physiological (pH 7.4) and tumorigenic (pH 6.5) media were used to mimic changes in extracellular pH within the TME.					
36087866	2	34	theme	TME	526:528	arg1	cells					541:545	the TME and cancer cells	522:545	cells	541:545	Traditional preclinical models, although useful to study biological processes, do not provide sufficient control over these physico-chemical properties, hence limiting the understanding of cause-effect relationships between the TME and cancer cells.					
36087866	10	35	theme	cancer	2077:2082	arg1	B-CSC					2095:2099	B-CSC	2095:2099	B-CSC	2095:2099	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	10	35	theme	cancer	2077:2082	arg1	cell					2089:2092	breast cancer stem cell	2070:2092	breast cancer stem cell (B-CSC) marker expression in a single in vitro model	2070:2145	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	4	36	theme	emerging	713:720	arg1	importance					722:731	their emerging importance	707:731	their emerging importance in disease physiology	707:753	With their emerging importance in disease physiology, it is essential to study the interplay between above-mentioned TME physico-chemical variables and B-CSC marker expression.					
36087866	0	37	theme	breast	80:85	arg1	stemness					99:106	breast cancer cell stemness	80:106	breast cancer cell stemness	80:106	Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.					
36087866	5	38	theme	breast	1142:1147	arg1	MDA-MB					1178:1183	MDA-MB 231	1178:1187	MDA-MB 231	1178:1187	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	38	theme	breast	1142:1147	arg1	MCF-7					1168:1172	MCF-7	1168:1172	MCF-7	1168:1172	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	38	theme	breast	1142:1147	arg1	lines					1161:1165	two separate breast cancer cell lines	1129:1165	two separate breast cancer cell lines (MCF-7 and MDA-MB 231)	1129:1188	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	39	theme	controlled	917:926	arg1	composition					980:990	composition	980:990	composition	980:990	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	39	theme	controlled	917:926	arg1	properties					945:954	controlled physico-chemical properties	917:954	controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH)	917:1006	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	39	theme	controlled	917:926	arg1	hydrogel					957:964	hydrogel stiffness and composition	957:990	hydrogel	957:964	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	12	40	theme	effective	2645:2653	arg1	identification					2655:2668	effective identification	2645:2668	effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment	2645:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	0	41	from	Role	0:3	arg1	stemness					99:106	breast cancer cell stemness	80:106	breast cancer cell stemness	80:106	Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.					
36087866	9	42	theme	physico-chemical	1869:1884	arg1	microenvironment					1886:1901	the physico-chemical microenvironment	1865:1901	the physico-chemical microenvironment	1865:1901	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	11	43	theme	tumour	2295:2300	arg1	microenvironment					2302:2317	the breast tumour microenvironment	2284:2317	the breast tumour microenvironment	2284:2317	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	0	44	theme	cell	94:97	arg1	stemness					99:106	breast cancer cell stemness	80:106	breast cancer cell stemness	80:106	Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.					
36087866	9	45	theme	acidic	1688:1693	arg1	6.5					1699:1701	6.5	1699:1701	6.5	1699:1701	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	9	45	theme	acidic	1688:1693	arg1	pH					1695:1696	acidic pH	1688:1696	acidic pH (6.5)	1688:1702	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	11	46	theme	varying	2208:2214	arg1	stiffness					2216:2224	varying stiffness	2208:2224	varying stiffness	2208:2224	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	5	47	from	proliferation	1079:1091	arg1	MDA-MB					1178:1183	MDA-MB 231	1178:1187	MDA-MB 231	1178:1187	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	47	from	proliferation	1079:1091	arg1	MCF-7					1168:1172	MCF-7	1168:1172	MCF-7	1168:1172	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	47	from	proliferation	1079:1091	arg1	lines					1161:1165	two separate breast cancer cell lines	1129:1165	two separate breast cancer cell lines (MCF-7 and MDA-MB 231)	1129:1188	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	12	48	theme	phenotypes	2682:2691	arg1	identification					2655:2668	effective identification	2645:2668	effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment	2645:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	5	49	dep	lines	1161:1165	arg1	MDA-MB					1178:1183	MDA-MB 231	1178:1187	MDA-MB 231	1178:1187	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	49	dep	lines	1161:1165	arg1	MCF-7					1168:1172	MCF-7	1168:1172	MCF-7	1168:1172	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	49	dep	lines	1161:1165	arg1	lines					1161:1165	two separate breast cancer cell lines	1129:1165	two separate breast cancer cell lines (MCF-7 and MDA-MB 231)	1129:1188	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	10	50	theme	single	2125:2130	arg1	model					2141:2145	a single in vitro model	2123:2145	a single in vitro model	2123:2145	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	2	51	theme	sufficient	392:401	arg1	control					403:409	sufficient control	392:409	sufficient control over these physico-chemical properties	392:448	Traditional preclinical models, although useful to study biological processes, do not provide sufficient control over these physico-chemical properties, hence limiting the understanding of cause-effect relationships between the TME and cancer cells.					
36087866	1	52	theme	fluid	286:290	arg1	flow					292:295	interstitial fluid flow	273:295	interstitial fluid flow	273:295	Several physico-chemical properties of the tumour microenvironment (TME) are dysregulated during tumour progression, such as tissue stiffness, extracellular pH and interstitial fluid flow.					
36087866	6	53	dep	14	1348:1349	arg1	to					1345:1346	to	1345:1346	to	1345:1346	Cells encapsulated in alginate-gelatin hydrogels varying in stiffness (2-10 kPa), density and adhesion ligand (gelatin) were perfused (500 µL/min) for up to 14 days.					
36087866	0	54	theme	stiffness	8:16	arg1	Role					0:3	Role	0:3	Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.	0:107	Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.					
36087866	1	55	theme	Several	109:115	arg1	properties					134:143	Several physico-chemical properties	109:143	Several physico-chemical properties of the tumour microenvironment (TME)	109:180	Several physico-chemical properties of the tumour microenvironment (TME) are dysregulated during tumour progression, such as tissue stiffness, extracellular pH and interstitial fluid flow.					
36087866	0	56	theme	microenvironment	60:75	arg1	stiffness					8:16	stiffness	8:16	stiffness	8:16	Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.					
36087866	0	56	theme	microenvironment	60:75	arg1	properties					39:48	physico-chemical properties	22:48	physico-chemical properties	22:48	Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.					
36087866	2	57	theme	physico-chemical	422:437	arg1	properties					439:448	these physico-chemical properties	416:448	these physico-chemical properties	416:448	Traditional preclinical models, although useful to study biological processes, do not provide sufficient control over these physico-chemical properties, hence limiting the understanding of cause-effect relationships between the TME and cancer cells.					
36087866	0	58	theme	physico-chemical	22:37	arg1	properties					39:48	physico-chemical properties	22:48	physico-chemical properties	22:48	Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.					
36087866	5	59	theme	cell	1156:1159	arg1	MDA-MB					1178:1183	MDA-MB 231	1178:1187	MDA-MB 231	1178:1187	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	59	theme	cell	1156:1159	arg1	MCF-7					1168:1172	MCF-7	1168:1172	MCF-7	1168:1172	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	59	theme	cell	1156:1159	arg1	lines					1161:1165	two separate breast cancer cell lines	1129:1165	two separate breast cancer cell lines (MCF-7 and MDA-MB 231)	1129:1188	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	9	60	theme	key	1711:1713	arg1	role					1715:1718	a key role	1709:1718	a key role	1709:1718	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	2	61	theme	cause-effect	487:498	arg1	relationships					500:512	cause-effect relationships	487:512	cause-effect relationships between the TME and cancer cells	487:545	Traditional preclinical models, although useful to study biological processes, do not provide sufficient control over these physico-chemical properties, hence limiting the understanding of cause-effect relationships between the TME and cancer cells.					
36087866	3	62	theme	tumour	643:648	arg1	progression					650:660	tumour progression	643:660	tumour progression	643:660	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36087866	4	63	theme	marker	860:865	arg1	expression					867:876	B-CSC marker expression	854:876	B-CSC marker expression	854:876	With their emerging importance in disease physiology, it is essential to study the interplay between above-mentioned TME physico-chemical variables and B-CSC marker expression.					
36087866	2	64	theme	biological	355:364	arg1	processes					366:374	biological processes	355:374	biological processes	355:374	Traditional preclinical models, although useful to study biological processes, do not provide sufficient control over these physico-chemical properties, hence limiting the understanding of cause-effect relationships between the TME and cancer cells.					
36087866	5	65	theme	breast	1045:1050	arg1	tissue					1052:1057	normal and tumour breast tissue	1027:1057	normal and tumour breast tissue	1027:1057	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	9	66	theme	B-CSCs	1723:1728	arg1	dynamics					1730:1737	B-CSCs dynamics	1723:1737	B-CSCs dynamics	1723:1737	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	10	67	from	time	2163:2166	arg1	impact					1998:2003	the impact	1994:2003	the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time	1994:2166	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	4	68	theme	above-mentioned	803:817	arg1	variables					840:848	above-mentioned TME physico-chemical variables	803:848	above-mentioned TME physico-chemical variables	803:848	With their emerging importance in disease physiology, it is essential to study the interplay between above-mentioned TME physico-chemical variables and B-CSC marker expression.					
36087866	5	69	used	used	1013:1016	arg2	models					905:910	3D in vitro models	893:910	3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH)	893:1006	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	1	70	theme	microenvironment	159:174	arg1	properties					134:143	Several physico-chemical properties	109:143	Several physico-chemical properties of the tumour microenvironment (TME)	109:180	Several physico-chemical properties of the tumour microenvironment (TME) are dysregulated during tumour progression, such as tissue stiffness, extracellular pH and interstitial fluid flow.					
36087866	8	71	theme	distinct	1509:1516	arg1	responses					1518:1526	distinct responses	1509:1526	distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology	1509:1628	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	4	72	theme	physico-chemical	823:838	arg1	variables					840:848	above-mentioned TME physico-chemical variables	803:848	above-mentioned TME physico-chemical variables	803:848	With their emerging importance in disease physiology, it is essential to study the interplay between above-mentioned TME physico-chemical variables and B-CSC marker expression.					
36087866	3	73	theme	Breast	548:553	arg1	population					593:602	a dynamic population	583:602	a dynamic population within the tumour	583:620	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36087866	3	73	theme	Breast	548:553	arg1	B-CSCs					574:579	B-CSCs	574:579	B-CSCs	574:579	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36087866	3	73	theme	Breast	548:553	arg1	cells					567:571	Breast cancer stem cells	548:571	Breast cancer stem cells (B-CSCs)	548:580	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36087866	8	74	theme	proliferation	1579:1591	arg1	terms					1570:1574	terms	1570:1574	terms of proliferation, cell aggregates size and morphology	1570:1628	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	7	75	from	changes	1430:1436	arg1	pH					1455:1456	extracellular pH	1441:1456	extracellular pH within the TME	1441:1471	Physiological (pH 7.4) and tumorigenic (pH 6.5) media were used to mimic changes in extracellular pH within the TME.					
36087866	7	76	used	used	1416:1419	arg2	media					1405:1409	Physiological (pH 7.4) and tumorigenic (pH 6.5) media	1357:1409	Physiological (pH 7.4) and tumorigenic (pH 6.5) media	1357:1409	Physiological (pH 7.4) and tumorigenic (pH 6.5) media were used to mimic changes in extracellular pH within the TME.					
36087866	12	77	from	presence	2700:2707	arg1	identification					2655:2668	effective identification	2645:2668	effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment	2645:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	12	78	theme	tumour	2721:2726	arg1	microenvironment					2745:2760	relevant tumour physico-chemical microenvironment	2712:2760	relevant tumour physico-chemical microenvironment	2712:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	8	79	theme	cell	1594:1597	arg1	proliferation					1579:1591	proliferation	1579:1591	proliferation	1579:1591	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	8	79	theme	cell	1594:1597	arg1	aggregates					1599:1608	cell aggregates size and morphology	1594:1628	cell aggregates size and morphology	1594:1628	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	8	79	theme	cell	1594:1597	arg1	morphology					1619:1628	morphology	1619:1628	morphology	1619:1628	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	8	79	theme	cell	1594:1597	arg1	size					1610:1613	size	1610:1613	size	1610:1613	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	1	80	theme	extracellular	252:264	arg1	pH					266:267	extracellular pH	252:267	extracellular pH	252:267	Several physico-chemical properties of the tumour microenvironment (TME) are dysregulated during tumour progression, such as tissue stiffness, extracellular pH and interstitial fluid flow.					
36087866	11	81	theme	3D	2184:2185	arg1	models					2196:2201	3D in vitro models	2184:2201	3D in vitro models with varying stiffness, extracellular pH and fluid flow	2184:2257	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	2	82	theme	Traditional	298:308	arg1	models					322:327	Traditional preclinical models	298:327	Traditional preclinical models	298:327	Traditional preclinical models, although useful to study biological processes, do not provide sufficient control over these physico-chemical properties, hence limiting the understanding of cause-effect relationships between the TME and cancer cells.					
36087866	11	83	from	effect	2363:2368	arg1	phenotypes					2396:2405	multiple breast cancer phenotypes	2373:2405	multiple breast cancer phenotypes	2373:2405	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	7	84	theme	tumorigenic	1384:1394	arg1	media					1405:1409	Physiological (pH 7.4) and tumorigenic (pH 6.5) media	1357:1409	Physiological (pH 7.4) and tumorigenic (pH 6.5) media	1357:1409	Physiological (pH 7.4) and tumorigenic (pH 6.5) media were used to mimic changes in extracellular pH within the TME.					
36087866	12	85	theme	microenvironment	2745:2760	arg1	presence					2700:2707	the presence	2696:2707	the presence of relevant tumour physico-chemical microenvironment	2696:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	3	86	theme	stem	562:565	arg1	population					593:602	a dynamic population	583:602	a dynamic population within the tumour	583:620	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36087866	3	86	theme	stem	562:565	arg1	B-CSCs					574:579	B-CSCs	574:579	B-CSCs	574:579	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36087866	3	86	theme	stem	562:565	arg1	cells					567:571	Breast cancer stem cells	548:571	Breast cancer stem cells (B-CSCs)	548:580	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36087866	10	87	dep	STATEMENT	1937:1945	arg1	evaluate					1985:1992	evaluate	1985:1992	evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time	1985:2166	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	9	88	theme	mesenchymal	1780:1790	arg1	cell					1804:1807	mesenchymal cancer stem cell	1780:1807	mesenchymal cancer stem cell (M-CSCs) marker expression	1780:1834	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	9	88	theme	mesenchymal	1780:1790	arg1	M-CSCs					1810:1815	M-CSCs	1810:1815	M-CSCs	1810:1815	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	11	89	dep	in	2187:2188	arg1	vitro					2190:2194	vitro	2190:2194	vitro	2190:2194	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	5	90	theme	3D	893:894	arg1	models					905:910	3D in vitro models	893:910	3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH)	893:1006	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	12	91	from	phenotypes	2682:2691	arg1	presence					2700:2707	the presence	2696:2707	the presence of relevant tumour physico-chemical microenvironment	2696:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	9	92	theme	stem	1799:1802	arg1	cell					1804:1807	mesenchymal cancer stem cell	1780:1807	mesenchymal cancer stem cell (M-CSCs) marker expression	1780:1834	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	9	92	theme	stem	1799:1802	arg1	M-CSCs					1810:1815	M-CSCs	1810:1815	M-CSCs	1810:1815	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	11	93	used	used	2263:2266	arg2	models					2196:2201	3D in vitro models	2184:2201	3D in vitro models with varying stiffness, extracellular pH and fluid flow	2184:2257	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	8	94	from	changes	1531:1537	arg1	factors					1559:1565	physico-chemical factors	1542:1565	physico-chemical factors in terms of proliferation, cell aggregates size and morphology	1542:1628	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	10	95	from	impact	1998:2003	arg1	expression					2109:2118	breast cancer stem cell (B-CSC) marker expression	2070:2118	breast cancer stem cell (B-CSC) marker expression in a single in vitro model	2070:2145	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	12	96	dep	suggest	2486:2492	arg1	open					2513:2516	open	2513:2516	suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment	2486:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	1	97	theme	tumour	206:211	arg1	progression					213:223	tumour progression	206:223	tumour progression	206:223	Several physico-chemical properties of the tumour microenvironment (TME) are dysregulated during tumour progression, such as tissue stiffness, extracellular pH and interstitial fluid flow.					
36087866	7	98	theme	Physiological	1357:1369	arg1	media					1405:1409	Physiological (pH 7.4) and tumorigenic (pH 6.5) media	1357:1409	Physiological (pH 7.4) and tumorigenic (pH 6.5) media	1357:1409	Physiological (pH 7.4) and tumorigenic (pH 6.5) media were used to mimic changes in extracellular pH within the TME.					
36087866	10	99	theme	properties	2025:2034	arg1	impact					1998:2003	the impact	1994:2003	the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time	1994:2166	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	10	100	theme	SIGNIFICANCE	1950:1961	arg1	STATEMENT					1937:1945	STATEMENT	1937:1945	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.	1937:2167	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	11	101	theme	direct	2356:2361	arg1	effect					2363:2368	their direct effect	2350:2368	their direct effect on multiple breast cancer phenotypes	2350:2405	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	10	102	theme	breast	2070:2075	arg1	B-CSC					2095:2099	B-CSC	2095:2099	B-CSC	2095:2099	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	10	102	theme	breast	2070:2075	arg1	cell					2089:2092	breast cancer stem cell	2070:2092	breast cancer stem cell (B-CSC) marker expression in a single in vitro model	2070:2145	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	1	103	theme	physico-chemical	117:132	arg1	properties					134:143	Several physico-chemical properties	109:143	Several physico-chemical properties of the tumour microenvironment (TME)	109:180	Several physico-chemical properties of the tumour microenvironment (TME) are dysregulated during tumour progression, such as tissue stiffness, extracellular pH and interstitial fluid flow.					
36087866	6	104	dep	perfused	1316:1323	arg1	µL/min					1330:1335	500 µL/min	1326:1335	500 µL/min	1326:1335	Cells encapsulated in alginate-gelatin hydrogels varying in stiffness (2-10 kPa), density and adhesion ligand (gelatin) were perfused (500 µL/min) for up to 14 days.					
36087866	9	105	theme	stiff	1649:1653	arg1	hydrogels					1665:1673	stiff and dense hydrogels	1649:1673	stiff and dense hydrogels (10 kPa)	1649:1682	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	9	105	theme	stiff	1649:1653	arg1	kPa					1679:1681	10 kPa	1676:1681	10 kPa	1676:1681	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	6	106	theme	alginate-gelatin	1213:1228	arg1	hydrogels					1230:1238	alginate-gelatin hydrogels	1213:1238	alginate-gelatin hydrogels varying in stiffness (2-10 kPa), density and adhesion ligand (gelatin)	1213:1309	Cells encapsulated in alginate-gelatin hydrogels varying in stiffness (2-10 kPa), density and adhesion ligand (gelatin) were perfused (500 µL/min) for up to 14 days.					
36087866	8	107	dep	aggregates	1599:1608	arg1	aggregates					1599:1608	cell aggregates size and morphology	1594:1628	cell aggregates size and morphology	1594:1628	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	8	107	dep	aggregates	1599:1608	arg1	morphology					1619:1628	morphology	1619:1628	morphology	1619:1628	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	8	107	dep	aggregates	1599:1608	arg1	size					1610:1613	size	1610:1613	size	1610:1613	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	10	108	theme	marker	2102:2107	arg1	expression					2109:2118	breast cancer stem cell (B-CSC) marker expression	2070:2118	breast cancer stem cell (B-CSC) marker expression in a single in vitro model	2070:2145	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	9	109	theme	dense	1659:1663	arg1	hydrogels					1665:1673	stiff and dense hydrogels	1649:1673	stiff and dense hydrogels (10 kPa)	1649:1682	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	9	109	theme	dense	1659:1663	arg1	kPa					1679:1681	10 kPa	1676:1681	10 kPa	1676:1681	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	11	110	theme	breast	2382:2387	arg1	phenotypes					2396:2405	multiple breast cancer phenotypes	2373:2405	multiple breast cancer phenotypes	2373:2405	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	11	111	theme	breast	2288:2293	arg1	microenvironment					2302:2317	the breast tumour microenvironment	2284:2317	the breast tumour microenvironment	2284:2317	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	4	112	theme	disease	736:742	arg1	physiology					744:753	disease physiology	736:753	disease physiology	736:753	With their emerging importance in disease physiology, it is essential to study the interplay between above-mentioned TME physico-chemical variables and B-CSC marker expression.					
36087866	1	113	theme	tumour	152:157	arg1	microenvironment					159:174	the tumour microenvironment	148:174	the tumour microenvironment (TME)	148:180	Several physico-chemical properties of the tumour microenvironment (TME) are dysregulated during tumour progression, such as tissue stiffness, extracellular pH and interstitial fluid flow.					
36087866	1	113	theme	tumour	152:157	arg1	TME					177:179	TME	177:179	TME	177:179	Several physico-chemical properties of the tumour microenvironment (TME) are dysregulated during tumour progression, such as tissue stiffness, extracellular pH and interstitial fluid flow.					
36087866	12	114	theme	specific	2673:2680	arg1	phenotypes					2682:2691	specific phenotypes	2673:2691	specific phenotypes in the presence of relevant tumour physico-chemical microenvironment	2673:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	10	115	theme	stem	2084:2087	arg1	B-CSC					2095:2099	B-CSC	2095:2099	B-CSC	2095:2099	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	10	115	theme	stem	2084:2087	arg1	cell					2089:2092	breast cancer stem cell	2070:2092	breast cancer stem cell (B-CSC) marker expression in a single in vitro model	2070:2145	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	5	116	from	morphology	1094:1103	arg1	MDA-MB					1178:1183	MDA-MB 231	1178:1187	MDA-MB 231	1178:1187	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	116	from	morphology	1094:1103	arg1	MCF-7					1168:1172	MCF-7	1168:1172	MCF-7	1168:1172	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	116	from	morphology	1094:1103	arg1	lines					1161:1165	two separate breast cancer cell lines	1129:1165	two separate breast cancer cell lines (MCF-7 and MDA-MB 231)	1129:1188	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	0	117	theme	cancer	87:92	arg1	stemness					99:106	breast cancer cell stemness	80:106	breast cancer cell stemness	80:106	Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.					
36087866	5	118	theme	separate	1133:1140	arg1	MDA-MB					1178:1183	MDA-MB 231	1178:1187	MDA-MB 231	1178:1187	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	118	theme	separate	1133:1140	arg1	MCF-7					1168:1172	MCF-7	1168:1172	MCF-7	1168:1172	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	118	theme	separate	1133:1140	arg1	lines					1161:1165	two separate breast cancer cell lines	1129:1165	two separate breast cancer cell lines (MCF-7 and MDA-MB 231)	1129:1188	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	119	theme	cancer	1149:1154	arg1	MDA-MB					1178:1183	MDA-MB 231	1178:1187	MDA-MB 231	1178:1187	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	119	theme	cancer	1149:1154	arg1	MCF-7					1168:1172	MCF-7	1168:1172	MCF-7	1168:1172	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	119	theme	cancer	1149:1154	arg1	lines					1161:1165	two separate breast cancer cell lines	1129:1165	two separate breast cancer cell lines (MCF-7 and MDA-MB 231)	1129:1188	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	120	with	models	905:910	arg1	composition					980:990	composition	980:990	composition	980:990	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	120	with	models	905:910	arg1	properties					945:954	controlled physico-chemical properties	917:954	controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH)	917:1006	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	120	with	models	905:910	arg1	hydrogel					957:964	hydrogel stiffness and composition	957:990	hydrogel	957:964	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	9	121	theme	microenvironment	1886:1901	arg1	impact					1855:1860	direct impact	1848:1860	direct impact of the physico-chemical microenvironment on disease onset and progression	1848:1934	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	5	122	dep	hydrogel	957:964	arg1	stiffness					966:974	stiffness	966:974	stiffness	966:974	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	123	theme	B-CSC	1109:1113	arg1	population					1115:1124	B-CSC population	1109:1124	B-CSC population	1109:1124	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	10	124	theme	cell	2089:2092	arg1	expression					2109:2118	breast cancer stem cell (B-CSC) marker expression	2070:2118	breast cancer stem cell (B-CSC) marker expression in a single in vitro model	2070:2145	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	9	125	from	impact	1855:1860	arg1	onset					1914:1918	disease onset	1906:1918	disease onset	1906:1918	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	9	125	from	impact	1855:1860	arg1	progression					1924:1934	progression	1924:1934	progression	1924:1934	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	9	126	theme	disease	1906:1912	arg1	onset					1914:1918	disease onset	1906:1918	disease onset	1906:1918	Most importantly, stiff and dense hydrogels (10 kPa) and acidic pH (6.5) play a key role in B-CSCs dynamics, increasing both epithelial (E-CSCs) and mesenchymal cancer stem cell (M-CSCs) marker expression, supporting direct impact of the physico-chemical microenvironment on disease onset and progression.					
36087866	5	127	dep	properties	945:954	arg1	composition					980:990	composition	980:990	composition	980:990	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	127	dep	properties	945:954	arg1	properties					945:954	controlled physico-chemical properties	917:954	controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH)	917:1006	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	127	dep	properties	945:954	arg1	pH					1004:1005	pH	1004:1005	pH	1004:1005	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	127	dep	properties	945:954	arg1	perfusion					993:1001	perfusion	993:1001	perfusion	993:1001	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	127	dep	properties	945:954	arg1	hydrogel					957:964	hydrogel stiffness and composition	957:990	hydrogel	957:964	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	11	128	dep	used	2263:2266	arg1	size					2443:2446	cell aggregate size	2428:2446	cell aggregate size	2428:2446	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	11	128	dep	used	2263:2266	arg1	markers					2469:2475	B-CSC markers	2463:2475	B-CSC markers	2463:2475	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	11	128	dep	used	2263:2266	arg1	proliferation					2413:2425	cell proliferation	2408:2425	cell proliferation	2408:2425	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	5	129	from	population	1115:1124	arg1	MDA-MB					1178:1183	MDA-MB 231	1178:1187	MDA-MB 231	1178:1187	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	129	from	population	1115:1124	arg1	MCF-7					1168:1172	MCF-7	1168:1172	MCF-7	1168:1172	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	5	129	from	population	1115:1124	arg1	lines					1161:1165	two separate breast cancer cell lines	1129:1165	two separate breast cancer cell lines (MCF-7 and MDA-MB 231)	1129:1188	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	12	130	from	identification	2655:2668	arg1	presence					2700:2707	the presence	2696:2707	the presence of relevant tumour physico-chemical microenvironment	2696:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	11	131	theme	cell	2428:2431	arg1	size					2443:2446	cell aggregate size	2428:2446	cell aggregate size	2428:2446	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	11	132	theme	B-CSC	2463:2467	arg1	markers					2469:2475	B-CSC markers	2463:2475	B-CSC markers	2463:2475	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	11	133	theme	extracellular	2227:2239	arg1	pH					2241:2242	extracellular pH	2227:2242	extracellular pH	2227:2242	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	12	134	theme	testing	2623:2629	arg1	platforms					2631:2639	testing platforms	2623:2639	testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment	2623:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	0	135	theme	properties	39:48	arg1	Role					0:3	Role	0:3	Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.	0:107	Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.					
36087866	5	136	theme	normal	1027:1032	arg1	tissue					1052:1057	normal and tumour breast tissue	1027:1057	normal and tumour breast tissue	1027:1057	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	4	137	from	importance	722:731	arg1	physiology					744:753	disease physiology	736:753	disease physiology	736:753	With their emerging importance in disease physiology, it is essential to study the interplay between above-mentioned TME physico-chemical variables and B-CSC marker expression.					
36087866	2	138	theme	relationships	500:512	arg1	understanding					470:482	the understanding	466:482	the understanding of cause-effect relationships between the TME and cancer cells	466:545	Traditional preclinical models, although useful to study biological processes, do not provide sufficient control over these physico-chemical properties, hence limiting the understanding of cause-effect relationships between the TME and cancer cells.					
36087866	8	139	theme	cell	1493:1496	arg1	lines					1498:1502	both cell lines	1488:1502	both cell lines	1488:1502	We found that both cell lines have distinct responses to changes in physico-chemical factors in terms of proliferation, cell aggregates size and morphology.					
36087866	5	140	dep	in	896:897	arg1	vitro					899:903	vitro	899:903	vitro	899:903	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	0	141	theme	tumour	53:58	arg1	microenvironment					60:75	tumour microenvironment	53:75	tumour microenvironment	53:75	Role of stiffness and physico-chemical properties of tumour microenvironment on breast cancer cell stemness.					
36087866	10	142	theme	in	2132:2133	arg1	model					2141:2145	a single in vitro model	2123:2145	a single in vitro model	2123:2145	STATEMENT OF SIGNIFICANCE: Currently no studies evaluate the impact of physico-chemical properties of the tumour microenvironment on breast cancer stem cell (B-CSC) marker expression in a single in vitro model and at the same time.					
36087866	5	143	theme	tumour	1038:1043	arg1	tissue					1052:1057	normal and tumour breast tissue	1027:1057	normal and tumour breast tissue	1027:1057	In this work, 3D in vitro models with controlled physico-chemical properties (hydrogel stiffness and composition, perfusion, pH) were used to mimic normal and tumour breast tissue to study changes in proliferation, morphology and B-CSC population in two separate breast cancer cell lines (MCF-7 and MDA-MB 231).					
36087866	3	144	theme	therapeutic	678:688	arg1	resistance					690:699	therapeutic resistance	678:699	therapeutic resistance	678:699	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36087866	4	145	theme	B-CSC	854:858	arg1	expression					867:876	B-CSC marker expression	854:876	B-CSC marker expression	854:876	With their emerging importance in disease physiology, it is essential to study the interplay between above-mentioned TME physico-chemical variables and B-CSC marker expression.					
36087866	12	146	theme	physico-chemical	2728:2743	arg1	microenvironment					2745:2760	relevant tumour physico-chemical microenvironment	2712:2760	relevant tumour physico-chemical microenvironment	2712:2760	Results suggest these models could open new ways of monitoring disease phenotypes, from the early-onset to progression, as well as being used as testing platforms for effective identification of specific phenotypes in the presence of relevant tumour physico-chemical microenvironment.					
36087866	7	147	theme	extracellular	1441:1453	arg1	pH					1455:1456	extracellular pH	1441:1456	extracellular pH within the TME	1441:1471	Physiological (pH 7.4) and tumorigenic (pH 6.5) media were used to mimic changes in extracellular pH within the TME.					
36087866	2	148	dep	provide	384:390	arg1	useful					339:344	useful	339:344	useful	339:344	Traditional preclinical models, although useful to study biological processes, do not provide sufficient control over these physico-chemical properties, hence limiting the understanding of cause-effect relationships between the TME and cancer cells.					
36087866	1	149	theme	tissue	234:239	arg1	stiffness					241:249	tissue stiffness	234:249	tissue stiffness	234:249	Several physico-chemical properties of the tumour microenvironment (TME) are dysregulated during tumour progression, such as tissue stiffness, extracellular pH and interstitial fluid flow.					
36087866	11	150	theme	cell	2408:2411	arg1	proliferation					2413:2425	cell proliferation	2408:2425	cell proliferation	2408:2425	In this study, 3D in vitro models with varying stiffness, extracellular pH and fluid flow are used to recapitulate the breast tumour microenvironment to evaluate for the first time their direct effect on multiple breast cancer phenotypes: cell proliferation, cell aggregate size and shape, and B-CSC markers.					
36087866	4	151	theme	TME	819:821	arg1	variables					840:848	above-mentioned TME physico-chemical variables	803:848	above-mentioned TME physico-chemical variables	803:848	With their emerging importance in disease physiology, it is essential to study the interplay between above-mentioned TME physico-chemical variables and B-CSC marker expression.					
36087866	3	152	theme	cancer	555:560	arg1	population					593:602	a dynamic population	583:602	a dynamic population within the tumour	583:620	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36087866	3	152	theme	cancer	555:560	arg1	B-CSCs					574:579	B-CSCs	574:579	B-CSCs	574:579	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36087866	3	152	theme	cancer	555:560	arg1	cells					567:571	Breast cancer stem cells	548:571	Breast cancer stem cells (B-CSCs)	548:580	Breast cancer stem cells (B-CSCs), a dynamic population within the tumour, are known to affect tumour progression, metastasis and therapeutic resistance.					
36758238	13	0	theme	cells	1632:1636	arg1	capacity					1620:1627	The capacity	1616:1627	The capacity of cells to proliferate	1616:1651	The capacity of cells to proliferate increased as the number of L-PRF components increased, indicating that L-PRF still exhibited biological activity after 3D printing.					
36758238	5	1	theme	better	644:649	arg1	properties					662:671	better mechanical properties	644:671	better mechanical properties	644:671	Moreover, chemical agents may be used for cross-linking for better mechanical properties, further increasing cytotoxicity.					
36758238	2	2	theme	platelet-rich	302:314	arg1	L-PRF					324:328	L-PRF	324:328	L-PRF	324:328	Here, we reported a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions.					
36758238	2	2	theme	platelet-rich	302:314	arg1	fibrin					316:321	lyophilized platelet-rich fibrin	290:321	lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions	290:405	Here, we reported a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions.					
36758238	11	3	theme	bone	1421:1424	arg1	repair					1426:1431	bone repair	1421:1431	bone repair	1421:1431	The antibacterial properties of the scaffolds were also verified, greatly reducing the risk of infection during bone repair.					
36758238	12	4	from	L-PRF	1503:1507	arg1	time					1476:1479	the release time	1464:1479	the release time of growth factor from L-PRF	1464:1507	It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 days of sustained release.					
36758238	12	4	from	L-PRF	1503:1507	arg1	factor					1491:1496	growth factor	1484:1496	growth factor from L-PRF	1484:1507	It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 days of sustained release.					
36758238	12	4	from	L-PRF	1503:1507	arg1	prolonged					1527:1535	prolonged	1527:1535	prolonged	1527:1535	It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 days of sustained release.					
36758238	10	5	theme	high	1239:1242	arg1	hydrophilicity					1244:1257	high hydrophilicity	1239:1257	high hydrophilicity	1239:1257	Meanwhile, the scaffolds had high hydrophilicity, porosity, and could be degraded stably in vitro.					
36758238	10	5	theme	high	1239:1242	arg1	porosity					1260:1267	porosity	1260:1267	porosity	1260:1267	Meanwhile, the scaffolds had high hydrophilicity, porosity, and could be degraded stably in vitro.					
36758238	2	6	theme	lyophilized	290:300	arg1	L-PRF					324:328	L-PRF	324:328	L-PRF	324:328	Here, we reported a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions.					
36758238	2	6	theme	lyophilized	290:300	arg1	fibrin					316:321	lyophilized platelet-rich fibrin	290:321	lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions	290:405	Here, we reported a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions.					
36758238	4	7	theme	CS	529:530	arg1	hydrogels					532:540	acid-soluble CS hydrogels	516:540	acid-soluble CS hydrogels	516:540	However, acid-soluble CS hydrogels often contain toxic and organic solvents.					
36758238	0	8	theme	platelet-rich	99:111	arg1	fibrin					113:118	lyophilized platelet-rich fibrin	87:118	lyophilized platelet-rich fibrin	87:118	3D printing of "green" thermo-sensitive chitosan-hydroxyapatite bone scaffold based on lyophilized platelet-rich fibrin.					
36758238	12	9	theme	factor	1491:1496	arg1	time					1476:1479	the release time	1464:1479	the release time of growth factor from L-PRF	1464:1507	It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 days of sustained release.					
36758238	12	9	theme	factor	1491:1496	arg1	prolonged					1527:1535	prolonged	1527:1535	prolonged	1527:1535	It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 days of sustained release.					
36758238	7	10	theme	extrusion-based	923:937	arg1	printing					939:946	extrusion-based printing	923:946	extrusion-based printing	923:946	Finally, the L-PRF-CS-HAP (P-C-H) composite scaffold was constructed by extrusion-based printing.					
36758238	3	11	theme	repair	487:492	arg1	environment					494:504	the bone repair environment	478:504	the bone repair environment	478:504	It also has strong mechanical properties to maintain the stability of the bone repair environment.					
36758238	13	12	theme	components	1686:1695	arg1	number					1670:1675	the number	1666:1675	the number of L-PRF components	1666:1695	The capacity of cells to proliferate increased as the number of L-PRF components increased, indicating that L-PRF still exhibited biological activity after 3D printing.					
36758238	9	13	with	trend	1102:1106	arg1	content					1130:1136	L-PRF content increasing	1124:1147	L-PRF content increasing	1124:1147	The compressive properties of the scaffolds exhibited a trend of decline with L-PRF content increasing, but all of them could meet the strength of cancellous bone.					
36758238	0	14	theme	lyophilized	87:97	arg1	fibrin					113:118	lyophilized platelet-rich fibrin	87:118	lyophilized platelet-rich fibrin	87:118	3D printing of "green" thermo-sensitive chitosan-hydroxyapatite bone scaffold based on lyophilized platelet-rich fibrin.					
36758238	8	15	theme	desirable	994:1002	arg1	printability					1004:1015	desirable printability	994:1015	desirable printability	994:1015	The results showed that the printing ink had desirable printability and temperature sensitivity.					
36758238	3	16	theme	environment	494:504	arg1	stability					465:473	the stability	461:473	the stability of the bone repair environment	461:504	It also has strong mechanical properties to maintain the stability of the bone repair environment.					
36758238	8	17	theme	temperature	1021:1031	arg1	sensitivity					1033:1043	temperature sensitivity	1021:1043	temperature sensitivity	1021:1043	The results showed that the printing ink had desirable printability and temperature sensitivity.					
36758238	6	18	dep	used	725:728	arg1	improved					786:793	improved	786:793	improved its mechanical properties	786:819	In this study, we used an alkali/urea dissolution system to dissolve CS, which improved its mechanical properties and made it thermo-sensitive.					
36758238	6	18	dep	used	725:728	arg1	made					825:828	made	825:828	made it thermo-sensitive	825:848	In this study, we used an alkali/urea dissolution system to dissolve CS, which improved its mechanical properties and made it thermo-sensitive.					
36758238	6	19	used	used	725:728	arg2	we					722:723	we	722:723	we	722:723	In this study, we used an alkali/urea dissolution system to dissolve CS, which improved its mechanical properties and made it thermo-sensitive.					
36758238	6	20	theme	dissolution	745:755	arg1	system					757:762	an alkali/urea dissolution system	730:762	an alkali/urea dissolution system	730:762	In this study, we used an alkali/urea dissolution system to dissolve CS, which improved its mechanical properties and made it thermo-sensitive.					
36758238	2	21	theme	chitosan	234:241	arg1	scaffolds					271:279	chitosan (CS) - hydroxyapatite (HAP) scaffolds	234:279	chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions	234:405	Here, we reported a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions.					
36758238	9	22	theme	bone	1204:1207	arg1	strength					1181:1188	the strength	1177:1188	the strength of cancellous bone	1177:1207	The compressive properties of the scaffolds exhibited a trend of decline with L-PRF content increasing, but all of them could meet the strength of cancellous bone.					
36758238	13	23	theme	L-PRF	1680:1684	arg1	components					1686:1695	L-PRF components	1680:1695	L-PRF components	1680:1695	The capacity of cells to proliferate increased as the number of L-PRF components increased, indicating that L-PRF still exhibited biological activity after 3D printing.					
36758238	12	24	from	time	1476:1479	arg1	L-PRF					1503:1507	L-PRF	1503:1507	L-PRF	1503:1507	It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 days of sustained release.					
36758238	6	25	theme	alkali/urea	733:743	arg1	system					757:762	an alkali/urea dissolution system	730:762	an alkali/urea dissolution system	730:762	In this study, we used an alkali/urea dissolution system to dissolve CS, which improved its mechanical properties and made it thermo-sensitive.					
36758238	12	26	theme	sustained	1597:1605	arg1	release					1607:1613	sustained release	1597:1613	sustained release	1597:1613	It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 days of sustained release.					
36758238	0	27	theme	3D	0:1	arg1	printing					3:10	3D printing	0:10	3D printing of "green" thermo-sensitive chitosan-hydroxyapatite bone scaffold based on lyophilized platelet-rich fibrin.	0:119	3D printing of "green" thermo-sensitive chitosan-hydroxyapatite bone scaffold based on lyophilized platelet-rich fibrin.					
36758238	11	28	theme	infection	1404:1412	arg1	risk					1396:1399	the risk	1392:1399	the risk of infection	1392:1412	The antibacterial properties of the scaffolds were also verified, greatly reducing the risk of infection during bone repair.					
36758238	5	29	theme	chemical	594:601	arg1	agents					603:608	chemical agents	594:608	chemical agents	594:608	Moreover, chemical agents may be used for cross-linking for better mechanical properties, further increasing cytotoxicity.					
36758238	8	30	theme	printing	977:984	arg1	ink					986:988	the printing ink	973:988	the printing ink	973:988	The results showed that the printing ink had desirable printability and temperature sensitivity.					
36758238	12	31	theme	release	1607:1613	arg1	days					1589:1592	35 days	1586:1592	35 days of sustained release	1586:1613	It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 days of sustained release.					
36758238	13	32	theme	3D	1772:1773	arg1	printing					1775:1782	3D printing	1772:1782	3D printing	1772:1782	The capacity of cells to proliferate increased as the number of L-PRF components increased, indicating that L-PRF still exhibited biological activity after 3D printing.					
36758238	9	33	theme	cancellous	1193:1202	arg1	bone					1204:1207	cancellous bone	1193:1207	cancellous bone	1193:1207	The compressive properties of the scaffolds exhibited a trend of decline with L-PRF content increasing, but all of them could meet the strength of cancellous bone.					
36758238	4	34	theme	toxic	556:560	arg1	solvents					574:581	toxic and organic solvents	556:581	toxic and organic solvents	556:581	However, acid-soluble CS hydrogels often contain toxic and organic solvents.					
36758238	12	35	theme	growth	1542:1547	arg1	factor					1549:1554	growth factor	1542:1554	growth factor	1542:1554	It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 days of sustained release.					
36758238	5	36	theme	mechanical	651:660	arg1	properties					662:671	better mechanical properties	644:671	better mechanical properties	644:671	Moreover, chemical agents may be used for cross-linking for better mechanical properties, further increasing cytotoxicity.					
36758238	2	37	theme	hydroxyapatite	250:263	arg1	scaffolds					271:279	chitosan (CS) - hydroxyapatite (HAP) scaffolds	234:279	chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions	234:405	Here, we reported a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions.					
36758238	9	38	theme	scaffolds	1080:1088	arg1	properties					1062:1071	The compressive properties	1046:1071	The compressive properties of the scaffolds	1046:1088	The compressive properties of the scaffolds exhibited a trend of decline with L-PRF content increasing, but all of them could meet the strength of cancellous bone.					
36758238	4	39	theme	acid-soluble	516:527	arg1	hydrogels					532:540	acid-soluble CS hydrogels	516:540	acid-soluble CS hydrogels	516:540	However, acid-soluble CS hydrogels often contain toxic and organic solvents.					
36758238	2	40	theme	respective	386:395	arg1	functions					397:405	their respective functions	380:405	their respective functions	380:405	Here, we reported a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions.					
36758238	13	41	theme	biological	1746:1755	arg1	activity					1757:1764	biological activity	1746:1764	biological activity	1746:1764	The capacity of cells to proliferate increased as the number of L-PRF components increased, indicating that L-PRF still exhibited biological activity after 3D printing.					
36758238	1	42	theme	urgent	158:163	arg1	problem					165:171	an urgent problem	155:171	an urgent problem in the field of bone repair	155:199	The critical bone defect is still an urgent problem in the field of bone repair.					
36758238	1	42	theme	urgent	158:163	arg1	defect					139:144	The critical bone defect	121:144	The critical bone defect	121:144	The critical bone defect is still an urgent problem in the field of bone repair.					
36758238	1	43	theme	bone	189:192	arg1	repair					194:199	bone repair	189:199	bone repair	189:199	The critical bone defect is still an urgent problem in the field of bone repair.					
36758238	0	44	theme	"	21:21	arg1	scaffold					69:76	"green" thermo-sensitive chitosan-hydroxyapatite bone scaffold	15:76	"green" thermo-sensitive chitosan-hydroxyapatite bone scaffold	15:76	3D printing of "green" thermo-sensitive chitosan-hydroxyapatite bone scaffold based on lyophilized platelet-rich fibrin.					
36758238	9	45	theme	compressive	1050:1060	arg1	properties					1062:1071	The compressive properties	1046:1071	The compressive properties of the scaffolds	1046:1088	The compressive properties of the scaffolds exhibited a trend of decline with L-PRF content increasing, but all of them could meet the strength of cancellous bone.					
36758238	1	46	theme	repair	194:199	arg1	field					180:184	the field	176:184	the field of bone repair	176:199	The critical bone defect is still an urgent problem in the field of bone repair.					
36758238	6	47	dep	it	830:831	arg1	thermo-sensitive					833:848	thermo-sensitive	833:848	it thermo-sensitive	830:848	In this study, we used an alkali/urea dissolution system to dissolve CS, which improved its mechanical properties and made it thermo-sensitive.					
36758238	2	48	theme	growth	354:359	arg1	factors					361:367	abundant growth factors	345:367	abundant growth factors	345:367	Here, we reported a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions.					
36758238	0	49	theme	green	16:20	arg1	scaffold					69:76	"green" thermo-sensitive chitosan-hydroxyapatite bone scaffold	15:76	"green" thermo-sensitive chitosan-hydroxyapatite bone scaffold	15:76	3D printing of "green" thermo-sensitive chitosan-hydroxyapatite bone scaffold based on lyophilized platelet-rich fibrin.					
36758238	11	50	theme	antibacterial	1313:1325	arg1	properties					1327:1336	The antibacterial properties	1309:1336	The antibacterial properties of the scaffolds	1309:1353	The antibacterial properties of the scaffolds were also verified, greatly reducing the risk of infection during bone repair.					
36758238	3	51	contain	has	416:418	arg1	It					408:409	It	408:409	It	408:409	It also has strong mechanical properties to maintain the stability of the bone repair environment.					
36758238	3	51	contain	has	416:418	arg2	properties					438:447	strong mechanical properties	420:447	strong mechanical properties	420:447	It also has strong mechanical properties to maintain the stability of the bone repair environment.					
36758238	4	52	contain	contain	548:554	arg2	solvents					574:581	toxic and organic solvents	556:581	toxic and organic solvents	556:581	However, acid-soluble CS hydrogels often contain toxic and organic solvents.					
36758238	4	52	contain	contain	548:554	arg1	hydrogels					532:540	acid-soluble CS hydrogels	516:540	acid-soluble CS hydrogels	516:540	However, acid-soluble CS hydrogels often contain toxic and organic solvents.					
36758238	2	53	theme	abundant	345:352	arg1	factors					361:367	abundant growth factors	345:367	abundant growth factors	345:367	Here, we reported a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions.					
36758238	0	54	theme	chitosan-hydroxyapatite	40:62	arg1	scaffold					69:76	"green" thermo-sensitive chitosan-hydroxyapatite bone scaffold	15:76	"green" thermo-sensitive chitosan-hydroxyapatite bone scaffold	15:76	3D printing of "green" thermo-sensitive chitosan-hydroxyapatite bone scaffold based on lyophilized platelet-rich fibrin.					
36758238	5	55	used	used	617:620	arg2	agents					603:608	chemical agents	594:608	chemical agents	594:608	Moreover, chemical agents may be used for cross-linking for better mechanical properties, further increasing cytotoxicity.					
36758238	12	56	theme	release	1468:1474	arg1	time					1476:1479	the release time	1464:1479	the release time of growth factor from L-PRF	1464:1507	It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 days of sustained release.					
36758238	12	56	theme	release	1468:1474	arg1	prolonged					1527:1535	prolonged	1527:1535	prolonged	1527:1535	It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 days of sustained release.					
36758238	6	57	theme	mechanical	799:808	arg1	properties					810:819	its mechanical properties	795:819	its mechanical properties	795:819	In this study, we used an alkali/urea dissolution system to dissolve CS, which improved its mechanical properties and made it thermo-sensitive.					
36758238	12	58	theme	growth	1484:1489	arg1	factor					1491:1496	growth factor	1484:1496	growth factor from L-PRF	1484:1507	It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 days of sustained release.					
36758238	0	59	theme	thermo-sensitive	23:38	arg1	scaffold					69:76	"green" thermo-sensitive chitosan-hydroxyapatite bone scaffold	15:76	"green" thermo-sensitive chitosan-hydroxyapatite bone scaffold	15:76	3D printing of "green" thermo-sensitive chitosan-hydroxyapatite bone scaffold based on lyophilized platelet-rich fibrin.					
36758238	3	60	theme	strong	420:425	arg1	properties					438:447	strong mechanical properties	420:447	strong mechanical properties	420:447	It also has strong mechanical properties to maintain the stability of the bone repair environment.					
36758238	9	61	theme	decline	1111:1117	arg1	trend					1102:1106	a trend	1100:1106	a trend of decline with L-PRF content increasing	1100:1147	The compressive properties of the scaffolds exhibited a trend of decline with L-PRF content increasing, but all of them could meet the strength of cancellous bone.					
36758238	1	62	theme	critical	125:132	arg1	problem					165:171	an urgent problem	155:171	an urgent problem in the field of bone repair	155:199	The critical bone defect is still an urgent problem in the field of bone repair.					
36758238	1	62	theme	critical	125:132	arg1	defect					139:144	The critical bone defect	121:144	The critical bone defect	121:144	The critical bone defect is still an urgent problem in the field of bone repair.					
36758238	7	63	theme	composite	885:893	arg1	scaffold					895:902	the L-PRF-CS-HAP (P-C-H) composite scaffold	860:902	the L-PRF-CS-HAP (P-C-H) composite scaffold	860:902	Finally, the L-PRF-CS-HAP (P-C-H) composite scaffold was constructed by extrusion-based printing.					
36758238	1	64	theme	bone	134:137	arg1	problem					165:171	an urgent problem	155:171	an urgent problem in the field of bone repair	155:199	The critical bone defect is still an urgent problem in the field of bone repair.					
36758238	1	64	theme	bone	134:137	arg1	defect					139:144	The critical bone defect	121:144	The critical bone defect	121:144	The critical bone defect is still an urgent problem in the field of bone repair.					
36758238	9	65	theme	increasing	1138:1147	arg1	content					1130:1136	L-PRF content increasing	1124:1147	L-PRF content increasing	1124:1147	The compressive properties of the scaffolds exhibited a trend of decline with L-PRF content increasing, but all of them could meet the strength of cancellous bone.					
36758238	3	66	theme	bone	482:485	arg1	environment					494:504	the bone repair environment	478:504	the bone repair environment	478:504	It also has strong mechanical properties to maintain the stability of the bone repair environment.					
36758238	13	67	dep	cells	1632:1636	arg1	proliferate					1641:1651	proliferate	1641:1651	to proliferate	1638:1651	The capacity of cells to proliferate increased as the number of L-PRF components increased, indicating that L-PRF still exhibited biological activity after 3D printing.					
36758238	11	68	theme	scaffolds	1345:1353	arg1	properties					1327:1336	The antibacterial properties	1309:1336	The antibacterial properties of the scaffolds	1309:1353	The antibacterial properties of the scaffolds were also verified, greatly reducing the risk of infection during bone repair.					
36758238	9	69	theme	L-PRF	1124:1128	arg1	content					1130:1136	L-PRF content increasing	1124:1147	L-PRF content increasing	1124:1147	The compressive properties of the scaffolds exhibited a trend of decline with L-PRF content increasing, but all of them could meet the strength of cancellous bone.					
36758238	3	70	theme	mechanical	427:436	arg1	properties					438:447	strong mechanical properties	420:447	strong mechanical properties	420:447	It also has strong mechanical properties to maintain the stability of the bone repair environment.					
36758238	2	71	theme	scaffolds	271:279	arg1	type					226:229	a new type	220:229	a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions	220:405	Here, we reported a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions.					
36758238	0	72	theme	scaffold	69:76	arg1	printing					3:10	3D printing	0:10	3D printing of "green" thermo-sensitive chitosan-hydroxyapatite bone scaffold based on lyophilized platelet-rich fibrin.	0:119	3D printing of "green" thermo-sensitive chitosan-hydroxyapatite bone scaffold based on lyophilized platelet-rich fibrin.					
36758238	8	73	contain	had	990:992	arg1	ink					986:988	the printing ink	973:988	the printing ink	973:988	The results showed that the printing ink had desirable printability and temperature sensitivity.					
36758238	8	73	contain	had	990:992	arg2	sensitivity					1033:1043	temperature sensitivity	1021:1043	temperature sensitivity	1021:1043	The results showed that the printing ink had desirable printability and temperature sensitivity.					
36758238	8	73	contain	had	990:992	arg2	printability					1004:1015	desirable printability	994:1015	desirable printability	994:1015	The results showed that the printing ink had desirable printability and temperature sensitivity.					
36758238	2	74	theme	new	222:224	arg1	type					226:229	a new type	220:229	a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions	220:405	Here, we reported a new type of chitosan (CS) - hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions.					
36758238	0	75	theme	bone	64:67	arg1	scaffold					69:76	"green" thermo-sensitive chitosan-hydroxyapatite bone scaffold	15:76	"green" thermo-sensitive chitosan-hydroxyapatite bone scaffold	15:76	3D printing of "green" thermo-sensitive chitosan-hydroxyapatite bone scaffold based on lyophilized platelet-rich fibrin.					
36758238	10	76	contain	had	1235:1237	arg1	scaffolds					1225:1233	the scaffolds	1221:1233	the scaffolds	1221:1233	Meanwhile, the scaffolds had high hydrophilicity, porosity, and could be degraded stably in vitro.					
36758238	10	76	contain	had	1235:1237	arg2	porosity					1260:1267	porosity	1260:1267	porosity	1260:1267	Meanwhile, the scaffolds had high hydrophilicity, porosity, and could be degraded stably in vitro.					
36758238	10	76	contain	had	1235:1237	arg1	Meanwhile					1210:1218	Meanwhile	1210:1218	Meanwhile	1210:1218	Meanwhile, the scaffolds had high hydrophilicity, porosity, and could be degraded stably in vitro.					
36758238	10	76	contain	had	1235:1237	arg2	hydrophilicity					1244:1257	high hydrophilicity	1239:1257	high hydrophilicity	1239:1257	Meanwhile, the scaffolds had high hydrophilicity, porosity, and could be degraded stably in vitro.					
36758238	1	77	from	problem	165:171	arg1	field					180:184	the field	176:184	the field of bone repair	176:199	The critical bone defect is still an urgent problem in the field of bone repair.					
36758238	4	78	theme	organic	566:572	arg1	solvents					574:581	toxic and organic solvents	556:581	toxic and organic solvents	556:581	However, acid-soluble CS hydrogels often contain toxic and organic solvents.					
35120583	1	0	theme	young	288:292	arg1	chickens					294:301	young chickens	288:301	young chickens	288:301	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE) causes severe disease in young chickens.					
35120583	10	1	from	SE	1559:1560	arg1	21 dpi					1594:1599	21 dpi	1594:1599	21 dpi	1594:1599	Higher relative abundance of streptococci was associated with reduced SE in ileal and caecal contents at 21 dpi.					
35120583	10	1	from	SE	1559:1560	arg1	contents					1582:1589	ileal and caecal contents	1565:1589	ileal and caecal contents at 21 dpi	1565:1599	Higher relative abundance of streptococci was associated with reduced SE in ileal and caecal contents at 21 dpi.					
35120583	4	2	theme	killer	658:663	arg1	responsiveness					632:645	responsiveness	632:645	responsiveness of natural killer (NK) and T cells in ileum and spleen	632:700	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	2	theme	killer	658:663	arg1	numbers					620:626	numbers	620:626	numbers	620:626	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	2	theme	killer	658:663	arg1	titers					728:733	SE-specific antibody titers	707:733	SE-specific antibody titers in serum	707:742	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	10	3	theme	relative	1496:1503	arg1	abundance					1505:1513	Higher relative abundance	1489:1513	Higher relative abundance of streptococci	1489:1529	Higher relative abundance of streptococci was associated with reduced SE in ileal and caecal contents at 21 dpi.					
35120583	0	4	with	infection	118:126	arg1	Enteritidis					182:192	Salmonella enterica serotype Enteritidis	153:192	Salmonella enterica serotype Enteritidis	153:192	Long-chain glucomannan supplementation modulates immune responsiveness, as well as intestinal microbiota, and impacts infection of broiler chickens with Salmonella enterica serotype Enteritidis.					
35120583	4	5	from	killer	658:663	arg1	ileum					685:689	ileum	685:689	ileum	685:689	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	5	from	killer	658:663	arg1	spleen					695:700	spleen	695:700	spleen	695:700	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	9	6	theme	relative	1362:1369	arg1	abundance					1371:1379	relative abundance	1362:1379	relative abundance of the commensal lactic acid bacteria (LAB)	1362:1423	Furthermore, relative abundance of the commensal lactic acid bacteria (LAB) significantly increased with GM supplementation post-infection.					
35120583	8	7	theme	NK	1139:1140	arg1	cells					1142:1146	intraepithelial NK cells	1123:1146	intraepithelial NK cells	1123:1146	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	8	8	theme	NK	1211:1212	arg1	7 dpi					1221:1225	7 dpi	1221:1225	7 dpi	1221:1225	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	8	8	theme	NK	1211:1212	arg1	numbers					1229:1235	numbers	1229:1235	numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi)	1229:1322	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	8	8	theme	NK	1211:1212	arg1	cells					1214:1218	intraepithelial NK cells	1195:1218	intraepithelial NK cells (7 dpi)	1195:1226	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	7	9	theme	SE	1016:1017	arg1	abundance					1003:1011	the relative abundance	990:1011	the relative abundance of SE based on DNA assessment	990:1041	In addition, the relative abundance of SE based on DNA assessment was significantly lower at 21 dpi upon GM supplementation.					
35120583	7	9	theme	SE	1016:1017	arg1	lower					1061:1065	lower	1061:1065	lower	1061:1065	In addition, the relative abundance of SE based on DNA assessment was significantly lower at 21 dpi upon GM supplementation.					
35120583	3	10	theme	age	564:566	arg1	days					556:559	seven days	550:559	seven days of age	550:566	In this study, chickens were fed long-chain glucomannan (GM) or standard diet and challenged with SE at seven days of age.					
35120583	9	11	theme	commensal	1388:1396	arg1	LAB					1420:1422	LAB	1420:1422	LAB	1420:1422	Furthermore, relative abundance of the commensal lactic acid bacteria (LAB) significantly increased with GM supplementation post-infection.					
35120583	9	11	theme	commensal	1388:1396	arg1	bacteria					1410:1417	the commensal lactic acid bacteria	1384:1417	the commensal lactic acid bacteria (LAB)	1384:1423	Furthermore, relative abundance of the commensal lactic acid bacteria (LAB) significantly increased with GM supplementation post-infection.					
35120583	10	12	theme	ileal	1565:1569	arg1	contents					1582:1589	ileal and caecal contents	1565:1589	ileal and caecal contents at 21 dpi	1565:1599	Higher relative abundance of streptococci was associated with reduced SE in ileal and caecal contents at 21 dpi.					
35120583	11	13	theme	commensal	1786:1794	arg1	LAB					1796:1798	the commensal LAB	1782:1798	the commensal LAB	1782:1798	Relative abundance of streptococci negatively correlated with SE counts and positively correlated with NK cell activation and SE-specific antibodies, which suggests involvement of the commensal LAB in NK cell responsiveness.					
35120583	0	14	theme	chickens	139:146	arg1	infection					118:126	infection	118:126	infection of broiler chickens with Salmonella enterica serotype Enteritidis	118:192	Long-chain glucomannan supplementation modulates immune responsiveness, as well as intestinal microbiota, and impacts infection of broiler chickens with Salmonella enterica serotype Enteritidis.					
35120583	9	15	theme	acid	1405:1408	arg1	LAB					1420:1422	LAB	1420:1422	LAB	1420:1422	Furthermore, relative abundance of the commensal lactic acid bacteria (LAB) significantly increased with GM supplementation post-infection.					
35120583	9	15	theme	acid	1405:1408	arg1	bacteria					1410:1417	the commensal lactic acid bacteria	1384:1417	the commensal lactic acid bacteria (LAB)	1384:1423	Furthermore, relative abundance of the commensal lactic acid bacteria (LAB) significantly increased with GM supplementation post-infection.					
35120583	0	16	theme	Salmonella	153:162	arg1	Enteritidis					182:192	Salmonella enterica serotype Enteritidis	153:192	Salmonella enterica serotype Enteritidis	153:192	Long-chain glucomannan supplementation modulates immune responsiveness, as well as intestinal microbiota, and impacts infection of broiler chickens with Salmonella enterica serotype Enteritidis.					
35120583	5	17	theme	cells	874:878	arg1	function					855:862	function	855:862	function	855:862	Microbiota compositions in ileum and caeca were determined, as well as correlations of these with numbers and function of immune cells.					
35120583	5	17	theme	cells	874:878	arg1	numbers					843:849	numbers	843:849	numbers	843:849	Microbiota compositions in ileum and caeca were determined, as well as correlations of these with numbers and function of immune cells.					
35120583	8	18	theme	antibodies	1304:1313	arg1	numbers					1229:1235	numbers	1229:1235	numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi)	1229:1322	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	8	18	theme	antibodies	1304:1313	arg1	cells					1214:1218	intraepithelial NK cells	1195:1218	intraepithelial NK cells (7 dpi)	1195:1226	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	11	19	theme	NK	1705:1706	arg1	activation					1713:1722	NK cell activation	1705:1722	NK cell activation	1705:1722	Relative abundance of streptococci negatively correlated with SE counts and positively correlated with NK cell activation and SE-specific antibodies, which suggests involvement of the commensal LAB in NK cell responsiveness.					
35120583	0	20	theme	serotype	173:180	arg1	Enteritidis					182:192	Salmonella enterica serotype Enteritidis	153:192	Salmonella enterica serotype Enteritidis	153:192	Long-chain glucomannan supplementation modulates immune responsiveness, as well as intestinal microbiota, and impacts infection of broiler chickens with Salmonella enterica serotype Enteritidis.					
35120583	5	21	theme	immune	867:872	arg1	cells					874:878	immune cells	867:878	immune cells	867:878	Microbiota compositions in ileum and caeca were determined, as well as correlations of these with numbers and function of immune cells.					
35120583	0	22	dep	Salmonella	153:162	arg1	enterica					164:171	enterica	164:171	enterica	164:171	Long-chain glucomannan supplementation modulates immune responsiveness, as well as intestinal microbiota, and impacts infection of broiler chickens with Salmonella enterica serotype Enteritidis.					
35120583	8	23	theme	intraepithelial	1240:1254	arg1	14 dpi					1280:1285	14 dpi	1280:1285	14 dpi	1280:1285	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	8	23	theme	intraepithelial	1240:1254	arg1	cells					1273:1277	intraepithelial cytotoxic CD8+ T cells	1240:1277	intraepithelial cytotoxic CD8+ T cells (14 dpi)	1240:1286	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	4	24	theme	cells	676:680	arg1	responsiveness					632:645	responsiveness	632:645	responsiveness of natural killer (NK) and T cells in ileum and spleen	632:700	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	24	theme	cells	676:680	arg1	numbers					620:626	numbers	620:626	numbers	620:626	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	24	theme	cells	676:680	arg1	titers					728:733	SE-specific antibody titers	707:733	SE-specific antibody titers in serum	707:742	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	11	25	theme	SE-specific	1728:1738	arg1	antibodies					1740:1749	SE-specific antibodies	1728:1749	SE-specific antibodies	1728:1749	Relative abundance of streptococci negatively correlated with SE counts and positively correlated with NK cell activation and SE-specific antibodies, which suggests involvement of the commensal LAB in NK cell responsiveness.					
35120583	0	26	theme	intestinal	83:92	arg1	microbiota					94:103	intestinal microbiota	83:103	intestinal microbiota	83:103	Long-chain glucomannan supplementation modulates immune responsiveness, as well as intestinal microbiota, and impacts infection of broiler chickens with Salmonella enterica serotype Enteritidis.					
35120583	9	27	theme	GM	1454:1455	arg1	post-infection					1473:1486	GM supplementation post-infection	1454:1486	GM supplementation post-infection	1454:1486	Furthermore, relative abundance of the commensal lactic acid bacteria (LAB) significantly increased with GM supplementation post-infection.					
35120583	8	28	theme	cells	1273:1277	arg1	numbers					1229:1235	numbers	1229:1235	numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi)	1229:1322	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	8	28	theme	cells	1273:1277	arg1	cells					1214:1218	intraepithelial NK cells	1195:1218	intraepithelial NK cells (7 dpi)	1195:1226	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	4	29	theme	SE-specific	707:717	arg1	titers					728:733	SE-specific antibody titers	707:733	SE-specific antibody titers in serum	707:742	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	5	30	theme	Microbiota	745:754	arg1	compositions					756:767	Microbiota compositions	745:767	Microbiota compositions in ileum and caeca	745:786	Microbiota compositions in ileum and caeca were determined, as well as correlations of these with numbers and function of immune cells.					
35120583	0	31	theme	Long-chain	0:9	arg1	supplementation					23:37	Long-chain glucomannan supplementation	0:37	Long-chain glucomannan supplementation	0:37	Long-chain glucomannan supplementation modulates immune responsiveness, as well as intestinal microbiota, and impacts infection of broiler chickens with Salmonella enterica serotype Enteritidis.					
35120583	4	32	theme	antibody	719:726	arg1	titers					728:733	SE-specific antibody titers	707:733	SE-specific antibody titers in serum	707:742	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	0	33	theme	immune	49:54	arg1	responsiveness					56:69	immune responsiveness	49:69	immune responsiveness	49:69	Long-chain glucomannan supplementation modulates immune responsiveness, as well as intestinal microbiota, and impacts infection of broiler chickens with Salmonella enterica serotype Enteritidis.					
35120583	11	34	theme	cell	1806:1809	arg1	responsiveness					1811:1824	NK cell responsiveness	1803:1824	NK cell responsiveness	1803:1824	Relative abundance of streptococci negatively correlated with SE counts and positively correlated with NK cell activation and SE-specific antibodies, which suggests involvement of the commensal LAB in NK cell responsiveness.					
35120583	10	35	from	21 dpi	1594:1599	arg1	contents					1582:1589	ileal and caecal contents	1565:1589	ileal and caecal contents at 21 dpi	1565:1599	Higher relative abundance of streptococci was associated with reduced SE in ileal and caecal contents at 21 dpi.					
35120583	10	35	from	21 dpi	1594:1599	arg1	SE					1559:1560	reduced SE	1551:1560	reduced SE in ileal and caecal contents at 21 dpi	1551:1599	Higher relative abundance of streptococci was associated with reduced SE in ileal and caecal contents at 21 dpi.					
35120583	5	36	with	correlations	816:827	arg1	function					855:862	function	855:862	function	855:862	Microbiota compositions in ileum and caeca were determined, as well as correlations of these with numbers and function of immune cells.					
35120583	5	36	with	correlations	816:827	arg1	numbers					843:849	numbers	843:849	numbers	843:849	Microbiota compositions in ileum and caeca were determined, as well as correlations of these with numbers and function of immune cells.					
35120583	2	37	theme	control	358:364	arg1	strategies					366:375	alternative SE control strategies	343:375	alternative SE control strategies such as nutritional solutions	343:405	Restriction on antibiotic use requires alternative SE control strategies such as nutritional solutions to improve the resistance of chickens.					
35120583	2	37	theme	control	358:364	arg1	solutions					397:405	nutritional solutions	385:405	nutritional solutions	385:405	Restriction on antibiotic use requires alternative SE control strategies such as nutritional solutions to improve the resistance of chickens.					
35120583	10	38	theme	Higher	1489:1494	arg1	abundance					1505:1513	Higher relative abundance	1489:1513	Higher relative abundance of streptococci	1489:1529	Higher relative abundance of streptococci was associated with reduced SE in ileal and caecal contents at 21 dpi.					
35120583	3	39	theme	long-chain	479:488	arg1	GM					503:504	GM	503:504	GM	503:504	In this study, chickens were fed long-chain glucomannan (GM) or standard diet and challenged with SE at seven days of age.					
35120583	3	39	theme	long-chain	479:488	arg1	glucomannan					490:500	long-chain glucomannan	479:500	long-chain glucomannan (GM)	479:505	In this study, chickens were fed long-chain glucomannan (GM) or standard diet and challenged with SE at seven days of age.					
35120583	1	40	dep	Salmonella	217:226	arg1	enterica					228:235	enterica	228:235	enterica	228:235	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE) causes severe disease in young chickens.					
35120583	2	41	theme	nutritional	385:395	arg1	solutions					397:405	nutritional solutions	385:405	nutritional solutions	385:405	Restriction on antibiotic use requires alternative SE control strategies such as nutritional solutions to improve the resistance of chickens.					
35120583	1	42	theme	severe	270:275	arg1	disease					277:283	severe disease	270:283	severe disease	270:283	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE) causes severe disease in young chickens.					
35120583	4	43	theme	21 days	576:582	arg1	dpi					600:602	dpi	600:602	dpi	600:602	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	43	theme	21 days	576:582	arg1	post-infection					584:597	21 days post-infection	576:597	21 days post-infection (dpi)	576:603	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	10	44	theme	streptococci	1518:1529	arg1	abundance					1505:1513	Higher relative abundance	1489:1513	Higher relative abundance of streptococci	1489:1529	Higher relative abundance of streptococci was associated with reduced SE in ileal and caecal contents at 21 dpi.					
35120583	12	45	theme	chickens	1959:1966	arg1	infection					1940:1948	SE infection	1937:1948	SE infection of young chickens	1937:1966	These results indicate that GM supplementation modulates the immune system, intestinal microbiota and impacts SE infection of young chickens.					
35120583	8	46	theme	CD8+	1266:1269	arg1	14 dpi					1280:1285	14 dpi	1280:1285	14 dpi	1280:1285	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	8	46	theme	CD8+	1266:1269	arg1	cells					1273:1277	intraepithelial cytotoxic CD8+ T cells	1240:1277	intraepithelial cytotoxic CD8+ T cells (14 dpi)	1240:1286	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	7	47	theme	DNA	1028:1030	arg1	assessment					1032:1041	DNA assessment	1028:1041	DNA assessment	1028:1041	In addition, the relative abundance of SE based on DNA assessment was significantly lower at 21 dpi upon GM supplementation.					
35120583	1	48	theme	zoonotic	199:206	arg1	pathogen					208:215	The zoonotic pathogen	195:215	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE)	195:261	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE) causes severe disease in young chickens.					
35120583	4	49	theme	natural	650:656	arg1	killer					658:663	natural killer	650:663	natural killer (NK)	650:668	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	49	theme	natural	650:656	arg1	NK					666:667	NK	666:667	NK	666:667	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	10	50	theme	reduced	1551:1557	arg1	SE					1559:1560	reduced SE	1551:1560	reduced SE in ileal and caecal contents at 21 dpi	1551:1599	Higher relative abundance of streptococci was associated with reduced SE in ileal and caecal contents at 21 dpi.					
35120583	11	51	theme	Relative	1602:1609	arg1	abundance					1611:1619	Relative abundance	1602:1619	Relative abundance of streptococci	1602:1635	Relative abundance of streptococci negatively correlated with SE counts and positively correlated with NK cell activation and SE-specific antibodies, which suggests involvement of the commensal LAB in NK cell responsiveness.					
35120583	2	52	theme	alternative	343:353	arg1	strategies					366:375	alternative SE control strategies	343:375	alternative SE control strategies such as nutritional solutions	343:405	Restriction on antibiotic use requires alternative SE control strategies such as nutritional solutions to improve the resistance of chickens.					
35120583	2	52	theme	alternative	343:353	arg1	solutions					397:405	nutritional solutions	385:405	nutritional solutions	385:405	Restriction on antibiotic use requires alternative SE control strategies such as nutritional solutions to improve the resistance of chickens.					
35120583	5	53	theme	these	832:836	arg1	correlations					816:827	correlations	816:827	correlations of these with numbers and function of immune cells	816:878	Microbiota compositions in ileum and caeca were determined, as well as correlations of these with numbers and function of immune cells.					
35120583	12	54	theme	SE	1937:1938	arg1	infection					1940:1948	SE infection	1937:1948	SE infection of young chickens	1937:1966	These results indicate that GM supplementation modulates the immune system, intestinal microbiota and impacts SE infection of young chickens.					
35120583	11	55	from	involvement	1767:1777	arg1	responsiveness					1811:1824	NK cell responsiveness	1803:1824	NK cell responsiveness	1803:1824	Relative abundance of streptococci negatively correlated with SE counts and positively correlated with NK cell activation and SE-specific antibodies, which suggests involvement of the commensal LAB in NK cell responsiveness.					
35120583	0	56	theme	broiler	131:137	arg1	chickens					139:146	broiler chickens	131:146	broiler chickens	131:146	Long-chain glucomannan supplementation modulates immune responsiveness, as well as intestinal microbiota, and impacts infection of broiler chickens with Salmonella enterica serotype Enteritidis.					
35120583	4	57	from	responsiveness	632:645	arg1	ileum					685:689	ileum	685:689	ileum	685:689	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	57	from	responsiveness	632:645	arg1	serum					738:742	serum	738:742	serum	738:742	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	57	from	responsiveness	632:645	arg1	spleen					695:700	spleen	695:700	spleen	695:700	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	1	58	theme	Salmonella	217:226	arg1	SE					259:260	SE	259:260	SE	259:260	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE) causes severe disease in young chickens.					
35120583	1	58	theme	Salmonella	217:226	arg1	Enteritidis					246:256	Salmonella enterica serotype Enteritidis	217:256	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE)	195:261	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE) causes severe disease in young chickens.					
35120583	8	59	theme	intraepithelial	1123:1137	arg1	cells					1142:1146	intraepithelial NK cells	1123:1146	intraepithelial NK cells	1123:1146	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	6	60	theme	control	908:914	arg1	group					916:920	the control group	904:920	the control group	904:920	Some of the samples in the control group had numerically higher CFUs than the GM-treated group.					
35120583	4	61	theme	T	674:674	arg1	cells					676:680	T cells	674:680	T cells in ileum and spleen	674:700	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	8	62	theme	intraepithelial	1195:1209	arg1	7 dpi					1221:1225	7 dpi	1221:1225	7 dpi	1221:1225	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	8	62	theme	intraepithelial	1195:1209	arg1	numbers					1229:1235	numbers	1229:1235	numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi)	1229:1322	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	8	62	theme	intraepithelial	1195:1209	arg1	cells					1214:1218	intraepithelial NK cells	1195:1218	intraepithelial NK cells (7 dpi)	1195:1226	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	7	63	theme	relative	994:1001	arg1	abundance					1003:1011	the relative abundance	990:1011	the relative abundance of SE based on DNA assessment	990:1041	In addition, the relative abundance of SE based on DNA assessment was significantly lower at 21 dpi upon GM supplementation.					
35120583	7	63	theme	relative	994:1001	arg1	lower					1061:1065	lower	1061:1065	lower	1061:1065	In addition, the relative abundance of SE based on DNA assessment was significantly lower at 21 dpi upon GM supplementation.					
35120583	4	64	from	numbers	620:626	arg1	ileum					685:689	ileum	685:689	ileum	685:689	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	64	from	numbers	620:626	arg1	serum					738:742	serum	738:742	serum	738:742	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	64	from	numbers	620:626	arg1	spleen					695:700	spleen	695:700	spleen	695:700	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	9	65	theme	lactic	1398:1403	arg1	LAB					1420:1422	LAB	1420:1422	LAB	1420:1422	Furthermore, relative abundance of the commensal lactic acid bacteria (LAB) significantly increased with GM supplementation post-infection.					
35120583	9	65	theme	lactic	1398:1403	arg1	bacteria					1410:1417	the commensal lactic acid bacteria	1384:1417	the commensal lactic acid bacteria (LAB)	1384:1423	Furthermore, relative abundance of the commensal lactic acid bacteria (LAB) significantly increased with GM supplementation post-infection.					
35120583	8	66	theme	cells	1142:1146	arg1	numbers					1112:1118	numbers	1112:1118	numbers of intraepithelial NK cells	1112:1146	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	12	67	theme	young	1953:1957	arg1	chickens					1959:1966	young chickens	1953:1966	young chickens	1953:1966	These results indicate that GM supplementation modulates the immune system, intestinal microbiota and impacts SE infection of young chickens.					
35120583	8	68	theme	cells	1214:1218	arg1	activation					1181:1190	activation	1181:1190	activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi)	1181:1322	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	11	69	theme	LAB	1796:1798	arg1	involvement					1767:1777	involvement	1767:1777	involvement of the commensal LAB in NK cell responsiveness	1767:1824	Relative abundance of streptococci negatively correlated with SE counts and positively correlated with NK cell activation and SE-specific antibodies, which suggests involvement of the commensal LAB in NK cell responsiveness.					
35120583	9	70	theme	bacteria	1410:1417	arg1	abundance					1371:1379	relative abundance	1362:1379	relative abundance of the commensal lactic acid bacteria (LAB)	1362:1423	Furthermore, relative abundance of the commensal lactic acid bacteria (LAB) significantly increased with GM supplementation post-infection.					
35120583	11	71	theme	cell	1708:1711	arg1	activation					1713:1722	NK cell activation	1705:1722	NK cell activation	1705:1722	Relative abundance of streptococci negatively correlated with SE counts and positively correlated with NK cell activation and SE-specific antibodies, which suggests involvement of the commensal LAB in NK cell responsiveness.					
35120583	2	72	from	Restriction	304:314	arg1	use					330:332	antibiotic use	319:332	antibiotic use	319:332	Restriction on antibiotic use requires alternative SE control strategies such as nutritional solutions to improve the resistance of chickens.					
35120583	6	73	theme	GM-treated	959:968	arg1	group					970:974	the GM-treated group	955:974	the GM-treated group	955:974	Some of the samples in the control group had numerically higher CFUs than the GM-treated group.					
35120583	12	74	theme	immune	1888:1893	arg1	system					1895:1900	the immune system	1884:1900	the immune system	1884:1900	These results indicate that GM supplementation modulates the immune system, intestinal microbiota and impacts SE infection of young chickens.					
35120583	12	74	theme	immune	1888:1893	arg1	microbiota					1914:1923	intestinal microbiota	1903:1923	intestinal microbiota	1903:1923	These results indicate that GM supplementation modulates the immune system, intestinal microbiota and impacts SE infection of young chickens.					
35120583	8	75	theme	cytotoxic	1256:1264	arg1	14 dpi					1280:1285	14 dpi	1280:1285	14 dpi	1280:1285	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	8	75	theme	cytotoxic	1256:1264	arg1	cells					1273:1277	intraepithelial cytotoxic CD8+ T cells	1240:1277	intraepithelial cytotoxic CD8+ T cells (14 dpi)	1240:1286	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	5	76	from	compositions	756:767	arg1	caeca					782:786	caeca	782:786	caeca	782:786	Microbiota compositions in ileum and caeca were determined, as well as correlations of these with numbers and function of immune cells.					
35120583	5	76	from	compositions	756:767	arg1	ileum					772:776	ileum	772:776	ileum	772:776	Microbiota compositions in ileum and caeca were determined, as well as correlations of these with numbers and function of immune cells.					
35120583	9	77	theme	supplementation	1457:1471	arg1	post-infection					1473:1486	GM supplementation post-infection	1454:1486	GM supplementation post-infection	1454:1486	Furthermore, relative abundance of the commensal lactic acid bacteria (LAB) significantly increased with GM supplementation post-infection.					
35120583	2	78	theme	antibiotic	319:328	arg1	use					330:332	antibiotic use	319:332	antibiotic use	319:332	Restriction on antibiotic use requires alternative SE control strategies such as nutritional solutions to improve the resistance of chickens.					
35120583	6	79	theme	higher	938:943	arg1	CFUs					945:948	numerically higher CFUs	926:948	numerically higher CFUs	926:948	Some of the samples in the control group had numerically higher CFUs than the GM-treated group.					
35120583	8	80	theme	T	1271:1271	arg1	14 dpi					1280:1285	14 dpi	1280:1285	14 dpi	1280:1285	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	8	80	theme	T	1271:1271	arg1	cells					1273:1277	intraepithelial cytotoxic CD8+ T cells	1240:1277	intraepithelial cytotoxic CD8+ T cells (14 dpi)	1240:1286	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	11	81	theme	NK	1803:1804	arg1	responsiveness					1811:1824	NK cell responsiveness	1803:1824	NK cell responsiveness	1803:1824	Relative abundance of streptococci negatively correlated with SE counts and positively correlated with NK cell activation and SE-specific antibodies, which suggests involvement of the commensal LAB in NK cell responsiveness.					
35120583	12	82	theme	GM	1855:1856	arg1	supplementation					1858:1872	GM supplementation	1855:1872	GM supplementation	1855:1872	These results indicate that GM supplementation modulates the immune system, intestinal microbiota and impacts SE infection of young chickens.					
35120583	4	83	from	titers	728:733	arg1	ileum					685:689	ileum	685:689	ileum	685:689	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	83	from	titers	728:733	arg1	serum					738:742	serum	738:742	serum	738:742	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	83	from	titers	728:733	arg1	spleen					695:700	spleen	695:700	spleen	695:700	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	0	84	theme	glucomannan	11:21	arg1	supplementation					23:37	Long-chain glucomannan supplementation	0:37	Long-chain glucomannan supplementation	0:37	Long-chain glucomannan supplementation modulates immune responsiveness, as well as intestinal microbiota, and impacts infection of broiler chickens with Salmonella enterica serotype Enteritidis.					
35120583	10	85	theme	caecal	1575:1580	arg1	contents					1582:1589	ileal and caecal contents	1565:1589	ileal and caecal contents at 21 dpi	1565:1599	Higher relative abundance of streptococci was associated with reduced SE in ileal and caecal contents at 21 dpi.					
35120583	8	86	theme	SE-specific	1292:1302	arg1	14 dpi					1316:1321	14 dpi	1316:1321	14 dpi	1316:1321	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	8	86	theme	SE-specific	1292:1302	arg1	antibodies					1304:1313	SE-specific antibodies	1292:1313	SE-specific antibodies (14 dpi)	1292:1322	At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher.					
35120583	7	87	theme	GM	1082:1083	arg1	supplementation					1085:1099	GM supplementation	1082:1099	GM supplementation	1082:1099	In addition, the relative abundance of SE based on DNA assessment was significantly lower at 21 dpi upon GM supplementation.					
35120583	1	88	theme	serotype	237:244	arg1	SE					259:260	SE	259:260	SE	259:260	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE) causes severe disease in young chickens.					
35120583	1	88	theme	serotype	237:244	arg1	Enteritidis					246:256	Salmonella enterica serotype Enteritidis	217:256	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE)	195:261	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE) causes severe disease in young chickens.					
35120583	12	89	theme	intestinal	1903:1912	arg1	system					1895:1900	the immune system	1884:1900	the immune system	1884:1900	These results indicate that GM supplementation modulates the immune system, intestinal microbiota and impacts SE infection of young chickens.					
35120583	12	89	theme	intestinal	1903:1912	arg1	microbiota					1914:1923	intestinal microbiota	1903:1923	intestinal microbiota	1903:1923	These results indicate that GM supplementation modulates the immune system, intestinal microbiota and impacts SE infection of young chickens.					
35120583	11	90	theme	streptococci	1624:1635	arg1	abundance					1611:1619	Relative abundance	1602:1619	Relative abundance of streptococci	1602:1635	Relative abundance of streptococci negatively correlated with SE counts and positively correlated with NK cell activation and SE-specific antibodies, which suggests involvement of the commensal LAB in NK cell responsiveness.					
35120583	1	91	dep	pathogen	208:215	arg1	SE					259:260	SE	259:260	SE	259:260	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE) causes severe disease in young chickens.					
35120583	1	91	dep	pathogen	208:215	arg1	Enteritidis					246:256	Salmonella enterica serotype Enteritidis	217:256	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE)	195:261	The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE) causes severe disease in young chickens.					
35120583	2	92	theme	chickens	436:443	arg1	resistance					422:431	the resistance	418:431	the resistance of chickens	418:443	Restriction on antibiotic use requires alternative SE control strategies such as nutritional solutions to improve the resistance of chickens.					
35120583	6	93	contain	had	922:924	arg1	samples					893:899	the samples	889:899	the samples in the control group	889:920	Some of the samples in the control group had numerically higher CFUs than the GM-treated group.					
35120583	6	93	contain	had	922:924	arg2	CFUs					945:948	numerically higher CFUs	926:948	numerically higher CFUs	926:948	Some of the samples in the control group had numerically higher CFUs than the GM-treated group.					
35120583	6	93	contain	had	922:924	arg1	Some					881:884	Some	881:884	Some	881:884	Some of the samples in the control group had numerically higher CFUs than the GM-treated group.					
35120583	3	94	theme	standard	510:517	arg1	diet					519:522	standard diet	510:522	standard diet	510:522	In this study, chickens were fed long-chain glucomannan (GM) or standard diet and challenged with SE at seven days of age.					
35120583	4	95	from	spleen	695:700	arg1	responsiveness					632:645	responsiveness	632:645	responsiveness of natural killer (NK) and T cells in ileum and spleen	632:700	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	95	from	spleen	695:700	arg1	numbers					620:626	numbers	620:626	numbers	620:626	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	95	from	spleen	695:700	arg1	titers					728:733	SE-specific antibody titers	707:733	SE-specific antibody titers in serum	707:742	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	6	96	from	samples	893:899	arg1	group					916:920	the control group	904:920	the control group	904:920	Some of the samples in the control group had numerically higher CFUs than the GM-treated group.					
35120583	11	97	theme	SE	1664:1665	arg1	counts					1667:1672	SE counts	1664:1672	SE counts	1664:1672	Relative abundance of streptococci negatively correlated with SE counts and positively correlated with NK cell activation and SE-specific antibodies, which suggests involvement of the commensal LAB in NK cell responsiveness.					
35120583	2	98	theme	SE	355:356	arg1	strategies					366:375	alternative SE control strategies	343:375	alternative SE control strategies such as nutritional solutions	343:405	Restriction on antibiotic use requires alternative SE control strategies such as nutritional solutions to improve the resistance of chickens.					
35120583	2	98	theme	SE	355:356	arg1	solutions					397:405	nutritional solutions	385:405	nutritional solutions	385:405	Restriction on antibiotic use requires alternative SE control strategies such as nutritional solutions to improve the resistance of chickens.					
35120583	4	99	from	ileum	685:689	arg1	responsiveness					632:645	responsiveness	632:645	responsiveness of natural killer (NK) and T cells in ileum and spleen	632:700	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	99	from	ileum	685:689	arg1	numbers					620:626	numbers	620:626	numbers	620:626	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	99	from	ileum	685:689	arg1	titers					728:733	SE-specific antibody titers	707:733	SE-specific antibody titers in serum	707:742	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	100	from	cells	676:680	arg1	ileum					685:689	ileum	685:689	ileum	685:689	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
35120583	4	100	from	cells	676:680	arg1	spleen					695:700	spleen	695:700	spleen	695:700	During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum.					
37141998	4	0	theme	sustainable	749:759	arg1	ability					774:780	excellent sustainable regeneration ability	739:780	excellent sustainable regeneration ability	739:780	Moreover, BC-MA displayed desirable adsorption capacity in diverse water environments coupled with excellent sustainable regeneration ability.					
37141998	3	1	theme	enlarged	501:508	arg1	area					518:521	enlarged surface area	501:521	enlarged surface area (256.8 m2·g-1)	501:536	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	3	1	theme	enlarged	501:508	arg1	m2·g-1					530:535	256.8 m2·g-1	524:535	256.8 m2·g-1	524:535	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	6	2	theme	hydrogen	1023:1030	arg1	interactions					978:989	π-π interactions	974:989	π-π interactions	974:989	The mechanisms proposed here mainly concerned π-π interactions, pore filling, complexation and hydrogen bonding.					
37141998	6	2	theme	hydrogen	1023:1030	arg1	bonding					1032:1038	hydrogen bonding	1023:1038	hydrogen bonding	1023:1038	The mechanisms proposed here mainly concerned π-π interactions, pore filling, complexation and hydrogen bonding.					
37141998	7	3	theme	waste	1165:1169	arg1	reuse					1180:1184	simultaneous waste resource reuse	1152:1184	simultaneous waste resource reuse	1152:1184	These findings suggested that the synthesis of modified biochar from bagasse would offer new opportunities for simultaneous waste resource reuse and water pollution control.					
37141998	3	4	theme	adsorption	429:438	arg1	sites					440:444	the abundant adsorption sites	416:444	the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups	416:569	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	7	5	theme	simultaneous	1152:1163	arg1	reuse					1180:1184	simultaneous waste resource reuse	1152:1184	simultaneous waste resource reuse	1152:1184	These findings suggested that the synthesis of modified biochar from bagasse would offer new opportunities for simultaneous waste resource reuse and water pollution control.					
37141998	3	6	theme	functional	553:562	arg1	groups					564:569	reinforced functional groups	542:569	reinforced functional groups	542:569	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	0	7	theme	tetracycline	93:104	arg1	removal					82:88	the sustained removal	68:88	the sustained removal of tetracycline in water	68:113	One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.					
37141998	1	8	contain	had	156:158	arg1	TC					130:131	TC	130:131	TC	130:131	Tetracycline (TC) and sugarcane bagasse had both exerted enormous strain on environmental security.					
37141998	1	8	contain	had	156:158	arg1	bagasse					148:154	sugarcane bagasse	138:154	sugarcane bagasse	138:154	Tetracycline (TC) and sugarcane bagasse had both exerted enormous strain on environmental security.					
37141998	1	8	contain	had	156:158	arg1	Tetracycline					116:127	Tetracycline	116:127	Tetracycline (TC)	116:132	Tetracycline (TC) and sugarcane bagasse had both exerted enormous strain on environmental security.					
37141998	1	8	contain	had	156:158	arg2	strain					182:187	both exerted enormous strain	160:187	both exerted enormous strain	160:187	Tetracycline (TC) and sugarcane bagasse had both exerted enormous strain on environmental security.					
37141998	4	9	theme	regeneration	761:772	arg1	ability					774:780	excellent sustainable regeneration ability	739:780	excellent sustainable regeneration ability	739:780	Moreover, BC-MA displayed desirable adsorption capacity in diverse water environments coupled with excellent sustainable regeneration ability.					
37141998	2	10	theme	bio-waste	279:287	arg1	bagasse					289:295	impregnating bio-waste bagasse	266:295	impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA)	266:349	In this work, new composite adsorbent designed by impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA) was innovatively brought forward for TC removal.					
37141998	4	11	theme	adsorption	676:685	arg1	capacity					687:694	desirable adsorption capacity	666:694	desirable adsorption capacity	666:694	Moreover, BC-MA displayed desirable adsorption capacity in diverse water environments coupled with excellent sustainable regeneration ability.					
37141998	2	12	theme	impregnating	266:277	arg1	bagasse					289:295	impregnating bio-waste bagasse	266:295	impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA)	266:349	In this work, new composite adsorbent designed by impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA) was innovatively brought forward for TC removal.					
37141998	1	13	theme	enormous	173:180	arg1	strain					182:187	both exerted enormous strain	160:187	both exerted enormous strain	160:187	Tetracycline (TC) and sugarcane bagasse had both exerted enormous strain on environmental security.					
37141998	5	14	theme	TC	809:810	arg1	process					798:804	The absorption process	783:804	The absorption process of TC by BC-MA	783:819	The absorption process of TC by BC-MA was spontaneous and endothermic, and the pivotal rate-limiting stage pertained to intraparticle diffusion.					
37141998	5	14	theme	TC	809:810	arg1	spontaneous					825:835	spontaneous	825:835	spontaneous	825:835	The absorption process of TC by BC-MA was spontaneous and endothermic, and the pivotal rate-limiting stage pertained to intraparticle diffusion.					
37141998	5	15	theme	absorption	787:796	arg1	process					798:804	The absorption process	783:804	The absorption process of TC by BC-MA	783:819	The absorption process of TC by BC-MA was spontaneous and endothermic, and the pivotal rate-limiting stage pertained to intraparticle diffusion.					
37141998	5	15	theme	absorption	787:796	arg1	spontaneous					825:835	spontaneous	825:835	spontaneous	825:835	The absorption process of TC by BC-MA was spontaneous and endothermic, and the pivotal rate-limiting stage pertained to intraparticle diffusion.					
37141998	1	16	theme	exerted	165:171	arg1	strain					182:187	both exerted enormous strain	160:187	both exerted enormous strain	160:187	Tetracycline (TC) and sugarcane bagasse had both exerted enormous strain on environmental security.					
37141998	7	17	from	bagasse	1110:1116	arg1	synthesis					1075:1083	the synthesis	1071:1083	the synthesis of modified biochar from bagasse	1071:1116	These findings suggested that the synthesis of modified biochar from bagasse would offer new opportunities for simultaneous waste resource reuse and water pollution control.					
37141998	7	17	from	bagasse	1110:1116	arg1	biochar					1097:1103	modified biochar	1088:1103	modified biochar from bagasse	1088:1116	These findings suggested that the synthesis of modified biochar from bagasse would offer new opportunities for simultaneous waste resource reuse and water pollution control.					
37141998	2	18	theme	double	329:334	arg1	BC-MA					344:348	BC-MA	344:348	BC-MA	344:348	In this work, new composite adsorbent designed by impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA) was innovatively brought forward for TC removal.					
37141998	2	18	theme	double	329:334	arg1	oxides					336:341	magnesium-aluminum layered double oxides	302:341	magnesium-aluminum layered double oxides (BC-MA)	302:349	In this work, new composite adsorbent designed by impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA) was innovatively brought forward for TC removal.					
37141998	2	19	theme	TC	388:389	arg1	removal					391:397	TC removal	388:397	TC removal	388:397	In this work, new composite adsorbent designed by impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA) was innovatively brought forward for TC removal.					
37141998	0	20	theme	One-pot	0:6	arg1	preparation					8:18	One-pot preparation	0:18	One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.	0:114	One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.					
37141998	7	21	theme	water	1190:1194	arg1	control					1206:1212	water pollution control	1190:1212	water pollution control	1190:1212	These findings suggested that the synthesis of modified biochar from bagasse would offer new opportunities for simultaneous waste resource reuse and water pollution control.					
37141998	7	22	theme	modified	1088:1095	arg1	biochar					1097:1103	modified biochar	1088:1103	modified biochar from bagasse	1088:1116	These findings suggested that the synthesis of modified biochar from bagasse would offer new opportunities for simultaneous waste resource reuse and water pollution control.					
37141998	2	23	theme	layered	321:327	arg1	BC-MA					344:348	BC-MA	344:348	BC-MA	344:348	In this work, new composite adsorbent designed by impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA) was innovatively brought forward for TC removal.					
37141998	2	23	theme	layered	321:327	arg1	oxides					336:341	magnesium-aluminum layered double oxides	302:341	magnesium-aluminum layered double oxides (BC-MA)	302:349	In this work, new composite adsorbent designed by impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA) was innovatively brought forward for TC removal.					
37141998	0	24	theme	layered	23:29	arg1	biochar					56:62	layered double oxides-engineered biochar	23:62	layered double oxides-engineered biochar	23:62	One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.					
37141998	6	25	theme	pore	992:995	arg1	interactions					978:989	π-π interactions	974:989	π-π interactions	974:989	The mechanisms proposed here mainly concerned π-π interactions, pore filling, complexation and hydrogen bonding.					
37141998	6	25	theme	pore	992:995	arg1	filling					997:1003	pore filling	992:1003	pore filling	992:1003	The mechanisms proposed here mainly concerned π-π interactions, pore filling, complexation and hydrogen bonding.					
37141998	7	26	theme	biochar	1097:1103	arg1	synthesis					1075:1083	the synthesis	1071:1083	the synthesis of modified biochar from bagasse	1071:1116	These findings suggested that the synthesis of modified biochar from bagasse would offer new opportunities for simultaneous waste resource reuse and water pollution control.					
37141998	2	27	theme	magnesium-aluminum	302:319	arg1	BC-MA					344:348	BC-MA	344:348	BC-MA	344:348	In this work, new composite adsorbent designed by impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA) was innovatively brought forward for TC removal.					
37141998	2	27	theme	magnesium-aluminum	302:319	arg1	oxides					336:341	magnesium-aluminum layered double oxides	302:341	magnesium-aluminum layered double oxides (BC-MA)	302:349	In this work, new composite adsorbent designed by impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA) was innovatively brought forward for TC removal.					
37141998	7	28	theme	new	1130:1132	arg1	opportunities					1134:1146	new opportunities	1130:1146	new opportunities for simultaneous waste resource reuse and water pollution control	1130:1212	These findings suggested that the synthesis of modified biochar from bagasse would offer new opportunities for simultaneous waste resource reuse and water pollution control.					
37141998	3	29	theme	mg	632:633	arg1	g-1					635:637	250.6 mg g-1	626:637	250.6 mg g-1	626:637	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	3	30	theme	surface	510:516	arg1	area					518:521	enlarged surface area	501:521	enlarged surface area (256.8 m2·g-1)	501:536	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	3	30	theme	surface	510:516	arg1	m2·g-1					530:535	256.8 m2·g-1	524:535	256.8 m2·g-1	524:535	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	1	31	theme	environmental	192:204	arg1	security					206:213	environmental security	192:213	environmental security	192:213	Tetracycline (TC) and sugarcane bagasse had both exerted enormous strain on environmental security.					
37141998	0	32	theme	oxides-engineered	38:54	arg1	biochar					56:62	layered double oxides-engineered biochar	23:62	layered double oxides-engineered biochar	23:62	One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.					
37141998	0	33	from	removal	82:88	arg1	water					109:113	water	109:113	water	109:113	One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.					
37141998	3	34	theme	adsorption	584:593	arg1	BC-MA					605:609	BC-MA	605:609	BC-MA for TC	605:616	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	3	34	theme	adsorption	584:593	arg1	amount					595:600	the maximum adsorption amount	572:600	the maximum adsorption amount of BC-MA for TC	572:616	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	3	35	theme	developed	458:466	arg1	structure					474:482	developed pores structure	458:482	developed pores structure (0.308 cm3·g-1)	458:498	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	3	35	theme	developed	458:466	arg1	cm3·g-1					491:497	0.308 cm3·g-1	485:497	0.308 cm3·g-1	485:497	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	0	36	theme	double	31:36	arg1	biochar					56:62	layered double oxides-engineered biochar	23:62	layered double oxides-engineered biochar	23:62	One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.					
37141998	6	37	theme	π-π	974:976	arg1	complexation					1006:1017	complexation	1006:1017	complexation	1006:1017	The mechanisms proposed here mainly concerned π-π interactions, pore filling, complexation and hydrogen bonding.					
37141998	6	37	theme	π-π	974:976	arg1	bonding					1032:1038	hydrogen bonding	1023:1038	hydrogen bonding	1023:1038	The mechanisms proposed here mainly concerned π-π interactions, pore filling, complexation and hydrogen bonding.					
37141998	6	37	theme	π-π	974:976	arg1	interactions					978:989	π-π interactions	974:989	π-π interactions	974:989	The mechanisms proposed here mainly concerned π-π interactions, pore filling, complexation and hydrogen bonding.					
37141998	6	37	theme	π-π	974:976	arg1	filling					997:1003	pore filling	992:1003	pore filling	992:1003	The mechanisms proposed here mainly concerned π-π interactions, pore filling, complexation and hydrogen bonding.					
37141998	3	38	theme	abundant	420:427	arg1	sites					440:444	the abundant adsorption sites	416:444	the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups	416:569	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	7	39	theme	pollution	1196:1204	arg1	control					1206:1212	water pollution control	1190:1212	water pollution control	1190:1212	These findings suggested that the synthesis of modified biochar from bagasse would offer new opportunities for simultaneous waste resource reuse and water pollution control.					
37141998	3	40	theme	pores	468:472	arg1	structure					474:482	developed pores structure	458:482	developed pores structure (0.308 cm3·g-1)	458:498	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	3	40	theme	pores	468:472	arg1	cm3·g-1					491:497	0.308 cm3·g-1	485:497	0.308 cm3·g-1	485:497	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	7	41	theme	resource	1171:1178	arg1	reuse					1180:1184	simultaneous waste resource reuse	1152:1184	simultaneous waste resource reuse	1152:1184	These findings suggested that the synthesis of modified biochar from bagasse would offer new opportunities for simultaneous waste resource reuse and water pollution control.					
37141998	5	42	theme	rate-limiting	870:882	arg1	stage					884:888	the pivotal rate-limiting stage	858:888	the pivotal rate-limiting stage	858:888	The absorption process of TC by BC-MA was spontaneous and endothermic, and the pivotal rate-limiting stage pertained to intraparticle diffusion.					
37141998	3	43	theme	250.6	626:630	arg1	mg					632:633	mg	632:633	mg	632:633	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	4	44	theme	diverse	699:705	arg1	environments					713:724	diverse water environments	699:724	diverse water environments coupled with excellent sustainable regeneration ability	699:780	Moreover, BC-MA displayed desirable adsorption capacity in diverse water environments coupled with excellent sustainable regeneration ability.					
37141998	0	45	theme	biochar	56:62	arg1	preparation					8:18	One-pot preparation	0:18	One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.	0:114	One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.					
37141998	3	46	theme	maximum	576:582	arg1	BC-MA					605:609	BC-MA	605:609	BC-MA for TC	605:616	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	3	46	theme	maximum	576:582	arg1	amount					595:600	the maximum adsorption amount	572:600	the maximum adsorption amount of BC-MA for TC	572:616	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	0	47	attach	removal	82:88	arg2	tetracycline					93:104	tetracycline	93:104	tetracycline	93:104	One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.					
37141998	0	47	attach	removal	82:88	arg3	water					109:113	water	109:113	water	109:113	One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.					
37141998	3	48	theme	reinforced	542:551	arg1	groups					564:569	reinforced functional groups	542:569	reinforced functional groups	542:569	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	4	49	theme	excellent	739:747	arg1	ability					774:780	excellent sustainable regeneration ability	739:780	excellent sustainable regeneration ability	739:780	Moreover, BC-MA displayed desirable adsorption capacity in diverse water environments coupled with excellent sustainable regeneration ability.					
37141998	4	50	theme	water	707:711	arg1	environments					713:724	diverse water environments	699:724	diverse water environments coupled with excellent sustainable regeneration ability	699:780	Moreover, BC-MA displayed desirable adsorption capacity in diverse water environments coupled with excellent sustainable regeneration ability.					
37141998	0	51	theme	sustained	72:80	arg1	removal					82:88	the sustained removal	68:88	the sustained removal of tetracycline in water	68:113	One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.					
37141998	4	52	theme	desirable	666:674	arg1	capacity					687:694	desirable adsorption capacity	666:694	desirable adsorption capacity	666:694	Moreover, BC-MA displayed desirable adsorption capacity in diverse water environments coupled with excellent sustainable regeneration ability.					
37141998	5	53	theme	pivotal	862:868	arg1	stage					884:888	the pivotal rate-limiting stage	858:888	the pivotal rate-limiting stage	858:888	The absorption process of TC by BC-MA was spontaneous and endothermic, and the pivotal rate-limiting stage pertained to intraparticle diffusion.					
37141998	2	54	with	bagasse	289:295	arg1	BC-MA					344:348	BC-MA	344:348	BC-MA	344:348	In this work, new composite adsorbent designed by impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA) was innovatively brought forward for TC removal.					
37141998	2	54	with	bagasse	289:295	arg1	oxides					336:341	magnesium-aluminum layered double oxides	302:341	magnesium-aluminum layered double oxides (BC-MA)	302:349	In this work, new composite adsorbent designed by impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA) was innovatively brought forward for TC removal.					
37141998	3	55	theme	BC-MA	605:609	arg1	BC-MA					605:609	BC-MA	605:609	BC-MA for TC	605:616	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	3	55	theme	BC-MA	605:609	arg1	amount					595:600	the maximum adsorption amount	572:600	the maximum adsorption amount of BC-MA for TC	572:616	Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1.					
37141998	1	56	theme	sugarcane	138:146	arg1	bagasse					148:154	sugarcane bagasse	138:154	sugarcane bagasse	138:154	Tetracycline (TC) and sugarcane bagasse had both exerted enormous strain on environmental security.					
37141998	7	57	from	synthesis	1075:1083	arg1	bagasse					1110:1116	bagasse	1110:1116	bagasse	1110:1116	These findings suggested that the synthesis of modified biochar from bagasse would offer new opportunities for simultaneous waste resource reuse and water pollution control.					
36738969	9	0	theme	PEO	1147:1149	arg1	nanofibers					1151:1160	PEO nanofibers	1147:1160	PEO nanofibers	1147:1160	Spores were rapidly released from PEO nanofibers, while presence of alginate in the nanofibers prolonged their release.					
36738969	13	1	theme	promising	1792:1800	arg1	approach					1814:1821	a promising therapeutic approach	1790:1821	a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease	1790:2031	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	10	2	theme	mat	1314:1316	arg1	transformation					1282:1295	transformation	1282:1295	transformation of the nanofiber mat into a hydrogel and polymer erosion mediating spore release kinetics	1282:1385	All formulations exhibited swelling, followed by transformation of the nanofiber mat into a hydrogel and polymer erosion mediating spore release kinetics.					
36738969	7	3	theme	strains	785:791	arg1	Spores					762:767	Spores	762:767	Spores of two Bacillus strains	762:791	Spores of two Bacillus strains were incorporated individually or in combination into hydrophilic poly(ethylene oxide) (PEO) and composite PEO/alginate nanofibers.					
36738969	13	4	theme	probiotic	1926:1934	arg1	bacteria					1936:1943	probiotic bacteria	1926:1943	probiotic bacteria	1926:1943	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	9	5	attach	released	1133:1140	arg2	Spores					1113:1118	Spores	1113:1118	Spores	1113:1118	Spores were rapidly released from PEO nanofibers, while presence of alginate in the nanofibers prolonged their release.					
36738969	9	5	attach	released	1133:1140	arg1	nanofibers					1151:1160	PEO nanofibers	1147:1160	PEO nanofibers	1147:1160	Spores were rapidly released from PEO nanofibers, while presence of alginate in the nanofibers prolonged their release.					
36738969	13	6	theme	therapeutic	1802:1812	arg1	approach					1814:1821	a promising therapeutic approach	1790:1821	a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease	1790:2031	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	11	7	theme	Bacillus	1405:1412	arg1	strains					1414:1420	Bacillus strains	1405:1420	The investigated Bacillus strains	1388:1420	The investigated Bacillus strains released metabolites, which were not cytotoxic to peripheral blood mononuclear cells (PBMCs) in vitro.					
36738969	6	8	theme	immunomodulatory	664:679	arg1	activities					681:690	immunomodulatory activities	664:690	immunomodulatory activities	664:690	The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics.					
36738969	12	9	dep	activity	1577:1584	arg1	expression					1680:1689	expression	1680:1689	expression of proinflammatory cytokines	1680:1718	Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines.					
36738969	11	10	theme	peripheral	1472:1481	arg1	PBMCs					1508:1512	PBMCs	1508:1512	PBMCs	1508:1512	The investigated Bacillus strains released metabolites, which were not cytotoxic to peripheral blood mononuclear cells (PBMCs) in vitro.					
36738969	11	10	theme	peripheral	1472:1481	arg1	cells					1501:1505	peripheral blood mononuclear cells	1472:1505	peripheral blood mononuclear cells (PBMCs)	1472:1513	The investigated Bacillus strains released metabolites, which were not cytotoxic to peripheral blood mononuclear cells (PBMCs) in vitro.					
36738969	8	11	theme	storage	1084:1090	arg1	6 months					1072:1079	6 months	1072:1079	6 months	1072:1079	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	8	11	theme	storage	1084:1090	arg1	electrospinning					1052:1066	electrospinning	1052:1066	electrospinning	1052:1066	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	12	12	from	activity	1577:1584	arg1	PBMCs					1650:1654	PBMCs	1650:1654	PBMCs	1650:1654	Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines.					
36738969	13	13	theme	microbiota	1910:1919	arg1	modulation					1886:1895	modulation	1886:1895	modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease	1886:2031	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	6	14	theme	individuals	561:571	arg1	microbiota					539:548	the oral microbiota	530:548	the oral microbiota of healthy individuals	530:571	The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics.					
36738969	13	15	from	disease	2025:2031	arg1	recovery					2007:2014	recovery	2007:2014	recovery from the disease	2007:2031	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	12	16	theme	proinflammatory	1694:1708	arg1	cytokines					1710:1718	proinflammatory cytokines	1694:1718	proinflammatory cytokines	1694:1718	Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines.					
36738969	8	17	theme	nanofiber	929:937	arg1	mats					939:942	The nanofiber mats	925:942	The nanofiber mats	925:942	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	7	18	theme	Bacillus	776:783	arg1	strains					785:791	two Bacillus strains	772:791	two Bacillus strains	772:791	Spores of two Bacillus strains were incorporated individually or in combination into hydrophilic poly(ethylene oxide) (PEO) and composite PEO/alginate nanofibers.					
36738969	6	19	theme	healthy	553:559	arg1	individuals					561:571	healthy individuals	553:571	healthy individuals	553:571	The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics.					
36738969	10	20	theme	polymer	1338:1344	arg1	erosion					1346:1352	polymer erosion	1338:1352	polymer erosion	1338:1352	All formulations exhibited swelling, followed by transformation of the nanofiber mat into a hydrogel and polymer erosion mediating spore release kinetics.					
36738969	8	21	theme	room	1095:1098	arg1	temperature					1100:1110	room temperature	1095:1110	room temperature	1095:1110	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	9	22	theme	alginate	1181:1188	arg1	presence					1169:1176	presence	1169:1176	presence of alginate in the nanofibers	1169:1206	Spores were rapidly released from PEO nanofibers, while presence of alginate in the nanofibers prolonged their release.					
36738969	2	23	theme	dysbiosis	320:328	arg1	treatment					307:315	targeted treatment	298:315	targeted treatment of dysbiosis	298:328	Probiotics represent a promising approach for targeted treatment of dysbiosis in biofilm and modulation of host immune response.					
36738969	10	24	theme	spore	1364:1368	arg1	kinetics					1378:1385	spore release kinetics	1364:1385	spore release kinetics	1364:1385	All formulations exhibited swelling, followed by transformation of the nanofiber mat into a hydrogel and polymer erosion mediating spore release kinetics.					
36738969	3	25	theme	interdisciplinary	389:405	arg1	study					407:411	this interdisciplinary study	384:411	this interdisciplinary study	384:411	In this interdisciplinary study, nanofibers with two autochthonous Bacillus strains 27.3.					
36738969	1	26	theme	Biofilm-associated	99:116	arg1	diseases					118:125	Biofilm-associated diseases	99:125	Biofilm-associated diseases such as periodontitis	99:147	Biofilm-associated diseases such as periodontitis are widespread and challenging to treat which calls for new strategies for their effective management.					
36738969	1	26	theme	Biofilm-associated	99:116	arg1	periodontitis					135:147	periodontitis	135:147	periodontitis	135:147	Biofilm-associated diseases such as periodontitis are widespread and challenging to treat which calls for new strategies for their effective management.					
36738969	7	27	theme	composite	890:898	arg1	nanofibers					913:922	composite PEO/alginate nanofibers	890:922	composite PEO/alginate nanofibers	890:922	Spores of two Bacillus strains were incorporated individually or in combination into hydrophilic poly(ethylene oxide) (PEO) and composite PEO/alginate nanofibers.					
36738969	1	28	theme	new	205:207	arg1	strategies					209:218	new strategies	205:218	new strategies for their effective management	205:249	Biofilm-associated diseases such as periodontitis are widespread and challenging to treat which calls for new strategies for their effective management.					
36738969	13	29	theme	local	1904:1908	arg1	microbiota					1910:1919	the local microbiota	1900:1919	the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease	1900:2031	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	2	30	theme	host	359:362	arg1	response					371:378	host immune response	359:378	host immune response	359:378	Probiotics represent a promising approach for targeted treatment of dysbiosis in biofilm and modulation of host immune response.					
36738969	7	31	theme	PEO/alginate	900:911	arg1	nanofibers					913:922	composite PEO/alginate nanofibers	890:922	composite PEO/alginate nanofibers	890:922	Spores of two Bacillus strains were incorporated individually or in combination into hydrophilic poly(ethylene oxide) (PEO) and composite PEO/alginate nanofibers.					
36738969	3	32	theme	autochthonous	434:446	arg1	strains					457:463	two autochthonous Bacillus strains 27.3	430:468	two autochthonous Bacillus strains 27.3	430:468	In this interdisciplinary study, nanofibers with two autochthonous Bacillus strains 27.3.					
36738969	9	33	attach	presence	1169:1176	arg1	nanofibers					1197:1206	the nanofibers	1193:1206	the nanofibers	1193:1206	Spores were rapidly released from PEO nanofibers, while presence of alginate in the nanofibers prolonged their release.					
36738969	9	33	attach	presence	1169:1176	arg2	alginate					1181:1188	alginate	1181:1188	alginate	1181:1188	Spores were rapidly released from PEO nanofibers, while presence of alginate in the nanofibers prolonged their release.					
36738969	11	34	theme	investigated	1392:1403	arg1	strains					1414:1420	Bacillus strains	1405:1420	The investigated Bacillus strains	1388:1420	The investigated Bacillus strains released metabolites, which were not cytotoxic to peripheral blood mononuclear cells (PBMCs) in vitro.					
36738969	13	35	theme	delivery	1763:1770	arg1	system					1772:1777	the developed nanofiber-based delivery system	1733:1777	the developed nanofiber-based delivery system	1733:1777	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	10	36	theme	release	1370:1376	arg1	kinetics					1378:1385	spore release kinetics	1364:1385	spore release kinetics	1364:1385	All formulations exhibited swelling, followed by transformation of the nanofiber mat into a hydrogel and polymer erosion mediating spore release kinetics.					
36738969	0	37	theme	Bacillus	26:33	arg1	strains					35:41	genotyped Bacillus strains	16:41	genotyped Bacillus strains	16:41	Nanofibers with genotyped Bacillus strains exhibiting antibacterial and immunomodulatory activity.					
36738969	12	38	theme	PBMC	1675:1678	arg1	inhibition					1661:1670	inhibition	1661:1670	inhibition of PBMC	1661:1678	Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines.					
36738969	12	38	theme	PBMC	1675:1678	arg1	effect					1640:1645	an antiproliferative effect	1619:1645	an antiproliferative effect on PBMCs	1619:1654	Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines.					
36738969	12	38	theme	PBMC	1675:1678	arg1	activity					1577:1584	antibacterial activity	1563:1584	antibacterial activity against two periodontopathogens	1563:1616	Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines.					
36738969	6	39	theme	resistance	735:744	arg1	factors					703:709	virulence factors	693:709	virulence factors	693:709	The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics.					
36738969	6	39	theme	resistance	735:744	arg1	transferability					716:730	transferability	716:730	transferability of resistance to antibiotics	716:759	The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics.					
36738969	6	39	theme	resistance	735:744	arg1	genes					624:628	genes	624:628	genes associated with antimicrobial and immunomodulatory activities	624:690	The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics.					
36738969	0	40	theme	genotyped	16:24	arg1	strains					35:41	genotyped Bacillus strains	16:41	genotyped Bacillus strains	16:41	Nanofibers with genotyped Bacillus strains exhibiting antibacterial and immunomodulatory activity.					
36738969	8	41	from	6 months	1072:1079	arg1	temperature					1100:1110	room temperature	1095:1110	room temperature	1095:1110	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	6	42	theme	oral	534:537	arg1	microbiota					539:548	the oral microbiota	530:548	the oral microbiota of healthy individuals	530:571	The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics.					
36738969	12	43	theme	antibacterial	1563:1575	arg1	activity					1577:1584	antibacterial activity	1563:1584	antibacterial activity against two periodontopathogens	1563:1616	Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines.					
36738969	8	44	theme	high	968:971	arg1	loading					973:979	a high loading	966:979	a high loading of viable spores (> 7 log CFU/mg)	966:1013	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	1	45	theme	effective	230:238	arg1	management					240:249	their effective management	224:249	their effective management	224:249	Biofilm-associated diseases such as periodontitis are widespread and challenging to treat which calls for new strategies for their effective management.					
36738969	7	46	theme	ethylene	864:871	arg1	poly					859:862	hydrophilic poly	847:862	hydrophilic poly(ethylene oxide) (PEO)	847:884	Spores of two Bacillus strains were incorporated individually or in combination into hydrophilic poly(ethylene oxide) (PEO) and composite PEO/alginate nanofibers.					
36738969	7	46	theme	ethylene	864:871	arg1	oxide					873:877	ethylene oxide	864:877	ethylene oxide	864:877	Spores of two Bacillus strains were incorporated individually or in combination into hydrophilic poly(ethylene oxide) (PEO) and composite PEO/alginate nanofibers.					
36738969	13	47	theme	developed	1737:1745	arg1	system					1772:1777	the developed nanofiber-based delivery system	1733:1777	the developed nanofiber-based delivery system	1733:1777	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	10	48	theme	nanofiber	1304:1312	arg1	mat					1314:1316	the nanofiber mat	1300:1316	the nanofiber mat	1300:1316	All formulations exhibited swelling, followed by transformation of the nanofiber mat into a hydrogel and polymer erosion mediating spore release kinetics.					
36738969	2	49	theme	promising	275:283	arg1	approach					285:292	a promising approach	273:292	a promising approach for targeted treatment of dysbiosis	273:328	Probiotics represent a promising approach for targeted treatment of dysbiosis in biofilm and modulation of host immune response.					
36738969	13	50	theme	immune	1954:1959	arg1	response					1961:1968	host immune response	1949:1968	host immune response	1949:1968	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	13	51	theme	biofilm-associated	1833:1850	arg1	disease					1852:1858	biofilm-associated disease	1833:1858	biofilm-associated disease	1833:1858	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	7	52	theme	hydrophilic	847:857	arg1	poly					859:862	hydrophilic poly	847:862	hydrophilic poly(ethylene oxide) (PEO)	847:884	Spores of two Bacillus strains were incorporated individually or in combination into hydrophilic poly(ethylene oxide) (PEO) and composite PEO/alginate nanofibers.					
36738969	7	52	theme	hydrophilic	847:857	arg1	oxide					873:877	ethylene oxide	864:877	ethylene oxide	864:877	Spores of two Bacillus strains were incorporated individually or in combination into hydrophilic poly(ethylene oxide) (PEO) and composite PEO/alginate nanofibers.					
36738969	7	52	theme	hydrophilic	847:857	arg1	PEO					881:883	PEO	881:883	PEO	881:883	Spores of two Bacillus strains were incorporated individually or in combination into hydrophilic poly(ethylene oxide) (PEO) and composite PEO/alginate nanofibers.					
36738969	13	53	theme	nanofiber-based	1747:1761	arg1	system					1772:1777	the developed nanofiber-based delivery system	1733:1777	the developed nanofiber-based delivery system	1733:1777	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	9	54	from	presence	1169:1176	arg1	nanofibers					1197:1206	the nanofibers	1193:1206	the nanofibers	1193:1206	Spores were rapidly released from PEO nanofibers, while presence of alginate in the nanofibers prolonged their release.					
36738969	3	55	theme	Bacillus	448:455	arg1	strains					457:463	two autochthonous Bacillus strains 27.3	430:468	two autochthonous Bacillus strains 27.3	430:468	In this interdisciplinary study, nanofibers with two autochthonous Bacillus strains 27.3.					
36738969	8	56	from	temperature	1100:1110	arg1	storage					1084:1090	storage	1084:1090	storage at room temperature	1084:1110	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	8	56	from	temperature	1100:1110	arg1	6 months					1072:1079	6 months	1072:1079	6 months	1072:1079	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	8	56	from	temperature	1100:1110	arg1	electrospinning					1052:1066	electrospinning	1052:1066	electrospinning	1052:1066	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	12	57	theme	antiproliferative	1622:1638	arg1	effect					1640:1645	an antiproliferative effect	1619:1645	an antiproliferative effect on PBMCs	1619:1654	Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines.					
36738969	0	58	theme	antibacterial	54:66	arg1	activity					89:96	antibacterial and immunomodulatory activity	54:96	antibacterial and immunomodulatory activity	54:96	Nanofibers with genotyped Bacillus strains exhibiting antibacterial and immunomodulatory activity.					
36738969	12	59	from	inhibition	1661:1670	arg1	PBMCs					1650:1654	PBMCs	1650:1654	PBMCs	1650:1654	Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines.					
36738969	8	60	theme	spores	991:996	arg1	loading					973:979	a high loading	966:979	a high loading of viable spores (> 7 log CFU/mg)	966:1013	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	12	61	from	effect	1640:1645	arg1	PBMCs					1650:1654	PBMCs	1650:1654	PBMCs	1650:1654	Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines.					
36738969	12	62	theme	cytokines	1710:1718	arg1	expression					1680:1689	expression	1680:1689	expression of proinflammatory cytokines	1680:1718	Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines.					
36738969	0	63	with	Nanofibers	0:9	arg1	strains					35:41	genotyped Bacillus strains	16:41	genotyped Bacillus strains	16:41	Nanofibers with genotyped Bacillus strains exhibiting antibacterial and immunomodulatory activity.					
36738969	2	64	theme	response	371:378	arg1	modulation					345:354	modulation	345:354	modulation	345:354	Probiotics represent a promising approach for targeted treatment of dysbiosis in biofilm and modulation of host immune response.					
36738969	2	64	theme	response	371:378	arg1	biofilm					333:339	biofilm	333:339	biofilm	333:339	Probiotics represent a promising approach for targeted treatment of dysbiosis in biofilm and modulation of host immune response.					
36738969	6	65	attach	isolated	516:523	arg2	strains					503:509	The strains	499:509	The strains	499:509	The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics.					
36738969	6	65	attach	isolated	516:523	arg1	microbiota					539:548	the oral microbiota	530:548	the oral microbiota of healthy individuals	530:571	The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics.					
36738969	8	66	theme	viable	984:989	arg1	CFU/mg					1007:1012	> 7 log CFU/mg	999:1012	> 7 log CFU/mg	999:1012	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	8	66	theme	viable	984:989	arg1	spores					991:996	viable spores	984:996	viable spores (> 7 log CFU/mg)	984:1013	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	2	67	theme	targeted	298:305	arg1	treatment					307:315	targeted treatment	298:315	targeted treatment of dysbiosis	298:328	Probiotics represent a promising approach for targeted treatment of dysbiosis in biofilm and modulation of host immune response.					
36738969	8	68	theme	>	999:999	arg1	CFU/mg					1007:1012	> 7 log CFU/mg	999:1012	> 7 log CFU/mg	999:1012	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	8	68	theme	>	999:999	arg1	spores					991:996	viable spores	984:996	viable spores (> 7 log CFU/mg)	984:1013	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	2	69	theme	immune	364:369	arg1	response					371:378	host immune response	359:378	host immune response	359:378	Probiotics represent a promising approach for targeted treatment of dysbiosis in biofilm and modulation of host immune response.					
36738969	11	70	theme	mononuclear	1489:1499	arg1	PBMCs					1508:1512	PBMCs	1508:1512	PBMCs	1508:1512	The investigated Bacillus strains released metabolites, which were not cytotoxic to peripheral blood mononuclear cells (PBMCs) in vitro.					
36738969	11	70	theme	mononuclear	1489:1499	arg1	cells					1501:1505	peripheral blood mononuclear cells	1472:1505	peripheral blood mononuclear cells (PBMCs)	1472:1513	The investigated Bacillus strains released metabolites, which were not cytotoxic to peripheral blood mononuclear cells (PBMCs) in vitro.					
36738969	13	71	theme	host	1949:1952	arg1	response					1961:1968	host immune response	1949:1968	host immune response	1949:1968	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	8	72	from	electrospinning	1052:1066	arg1	temperature					1100:1110	room temperature	1095:1110	room temperature	1095:1110	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	6	73	theme	virulence	693:701	arg1	factors					703:709	virulence factors	693:709	virulence factors	693:709	The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics.					
36738969	0	74	theme	immunomodulatory	72:87	arg1	activity					89:96	antibacterial and immunomodulatory activity	54:96	antibacterial and immunomodulatory activity	54:96	Nanofibers with genotyped Bacillus strains exhibiting antibacterial and immunomodulatory activity.					
36738969	13	75	with	microbiota	1910:1919	arg1	bacteria					1936:1943	probiotic bacteria	1926:1943	probiotic bacteria	1926:1943	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	13	75	with	microbiota	1910:1919	arg1	response					1961:1968	host immune response	1949:1968	host immune response	1949:1968	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	13	75	with	microbiota	1910:1919	arg1	metabolites					1981:1991	their metabolites	1975:1991	their metabolites	1975:1991	In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites, resulting in recovery from the disease.					
36738969	8	76	theme	log	1003:1005	arg1	CFU/mg					1007:1012	> 7 log CFU/mg	999:1012	> 7 log CFU/mg	999:1012	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	8	76	theme	log	1003:1005	arg1	spores					991:996	viable spores	984:996	viable spores (> 7 log CFU/mg)	984:1013	The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature.					
36738969	11	77	theme	blood	1483:1487	arg1	PBMCs					1508:1512	PBMCs	1508:1512	PBMCs	1508:1512	The investigated Bacillus strains released metabolites, which were not cytotoxic to peripheral blood mononuclear cells (PBMCs) in vitro.					
36738969	11	77	theme	blood	1483:1487	arg1	cells					1501:1505	peripheral blood mononuclear cells	1472:1505	peripheral blood mononuclear cells (PBMCs)	1472:1513	The investigated Bacillus strains released metabolites, which were not cytotoxic to peripheral blood mononuclear cells (PBMCs) in vitro.					
36462695	11	0	dep	Fourier	1367:1373	arg1	transform					1375:1383	transform	1375:1383	transform infrared spectroscopy spectra	1375:1413	Fourier transform infrared spectroscopy spectra relate this band to a group of nanocomposites.					
36462695	10	1	theme	243 nm	1279:1284	arg1	band					1297:1300	the 243 nm absorption band	1275:1300	the 243 nm absorption band corresponding to the nanocomposite's surface plasmon resonances	1275:1364	UV-vis spectra confirm the 243 nm absorption band corresponding to the nanocomposite's surface plasmon resonances.					
36462695	12	2	theme	TiO2-Fe2O3-Chitosan	1516:1534	arg1	nanocomposites					1536:1549	TiO2-Fe2O3-Chitosan nanocomposites	1516:1549	TiO2-Fe2O3-Chitosan nanocomposites	1516:1549	The findings of this work emphasize the importance of TiO2-Fe2O3-Chitosan nanocomposites for removing ibuprofen from wastewater.					
36462695	11	3	dep	transform	1375:1383	arg1	infrared					1385:1392	infrared	1385:1392	transform infrared spectroscopy spectra	1375:1413	Fourier transform infrared spectroscopy spectra relate this band to a group of nanocomposites.					
36462695	10	4	theme	absorption	1286:1295	arg1	band					1297:1300	the 243 nm absorption band	1275:1300	the 243 nm absorption band corresponding to the nanocomposite's surface plasmon resonances	1275:1364	UV-vis spectra confirm the 243 nm absorption band corresponding to the nanocomposite's surface plasmon resonances.					
36462695	0	5	theme	docking	104:110	arg1	studies					112:118	its molecular docking studies	90:118	its molecular docking studies	90:118	Porous nanocomposites for sorptive elimination of ibuprofen from synthetic wastewater and its molecular docking studies.					
36462695	8	6	theme	Thermodynamic	1037:1049	arg1	parameters					1051:1060	Thermodynamic parameters	1037:1060	Thermodynamic parameters for ibuprofen sorption	1037:1083	Thermodynamic parameters for ibuprofen sorption were carried out and the ΔH and ΔS was found to be 76.23 & 0.233.					
36462695	7	7	theme	rotational	1008:1017	arg1	speed					1019:1023	a rotational speed	1006:1023	a rotational speed of 200 rpm	1006:1034	Ibuprofen elimination was demonstrated to be most successful at a pH of 7.3, using 0.05 g of nanocomposites at a rotational speed of 200 rpm.					
36462695	2	8	from	conjunction	342:352	arg1	synthesis					291:299	the green synthesis	281:299	the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal	281:432	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	2	8	from	conjunction	342:352	arg1	aim					260:262	The aim	256:262	The aim of this study	256:276	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	2	9	from	nanocomposites	324:337	arg1	conjunction					342:352	conjunction	342:352	conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal	342:432	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	0	10	theme	molecular	94:102	arg1	studies					112:118	its molecular docking studies	90:118	its molecular docking studies	90:118	Porous nanocomposites for sorptive elimination of ibuprofen from synthetic wastewater and its molecular docking studies.					
36462695	7	11	theme	nanocomposites	988:1001	arg1	0.05 g					978:983	0.05 g	978:983	0.05 g of nanocomposites	978:1001	Ibuprofen elimination was demonstrated to be most successful at a pH of 7.3, using 0.05 g of nanocomposites at a rotational speed of 200 rpm.					
36462695	2	12	theme	green	285:289	arg1	synthesis					291:299	the green synthesis	281:299	the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal	281:432	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	2	12	theme	green	285:289	arg1	aim					260:262	The aim	256:262	The aim of this study	256:276	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	7	13	theme	7.3	967:969	arg1	pH					961:962	a pH	959:962	a pH of 7.3	959:969	Ibuprofen elimination was demonstrated to be most successful at a pH of 7.3, using 0.05 g of nanocomposites at a rotational speed of 200 rpm.					
36462695	10	14	theme	UV-vis	1252:1257	arg1	spectra					1259:1265	UV-vis spectra	1252:1265	UV-vis spectra	1252:1265	UV-vis spectra confirm the 243 nm absorption band corresponding to the nanocomposite's surface plasmon resonances.					
36462695	3	15	theme	Various	435:441	arg1	studies					460:466	Various characterization studies	435:466	Various characterization studies	435:466	Various characterization studies were performed for the synthesized nanocomposites.					
36462695	5	16	theme	Langmuir	673:680	arg1	isotherm					682:689	Langmuir isotherm	673:689	Langmuir isotherm	673:689	Equilibrium results show that adsorption process matches with Langmuir isotherm, demonstrating adsorption on the nanocomposite's homogenous surface and follows pseudo-first-order kinetics.					
36462695	6	17	theme	agitation	839:847	arg1	pH					815:816	pH	815:816	pH	815:816	Using the BBD, pH, adsorbent dose, and agitation speed were examined as adsorption parameters.					
36462695	6	17	theme	agitation	839:847	arg1	dose					829:832	adsorbent dose	819:832	adsorbent dose	819:832	Using the BBD, pH, adsorbent dose, and agitation speed were examined as adsorption parameters.					
36462695	6	17	theme	agitation	839:847	arg1	parameters					883:892	adsorption parameters	872:892	adsorption parameters	872:892	Using the BBD, pH, adsorbent dose, and agitation speed were examined as adsorption parameters.					
36462695	6	17	theme	agitation	839:847	arg1	speed					849:853	agitation speed	839:853	agitation speed	839:853	Using the BBD, pH, adsorbent dose, and agitation speed were examined as adsorption parameters.					
36462695	1	18	theme	aquatic	188:194	arg1	ecosystems					196:205	aquatic ecosystems	188:205	aquatic ecosystems	188:205	Pharmaceuticals are a new developing pollutant that is threatening aquatic ecosystems and impacting numerous species in the ecosystem.					
36462695	12	19	theme	work	1483:1486	arg1	findings					1466:1473	The findings	1462:1473	The findings of this work	1462:1486	The findings of this work emphasize the importance of TiO2-Fe2O3-Chitosan nanocomposites for removing ibuprofen from wastewater.					
36462695	5	20	theme	Equilibrium	611:621	arg1	results					623:629	Equilibrium results	611:629	Equilibrium results	611:629	Equilibrium results show that adsorption process matches with Langmuir isotherm, demonstrating adsorption on the nanocomposite's homogenous surface and follows pseudo-first-order kinetics.					
36462695	11	21	theme	spectroscopy	1394:1405	arg1	spectra					1407:1413	spectroscopy spectra	1394:1413	spectroscopy spectra	1394:1413	Fourier transform infrared spectroscopy spectra relate this band to a group of nanocomposites.					
36462695	8	22	theme	ibuprofen	1066:1074	arg1	sorption					1076:1083	ibuprofen sorption	1066:1083	ibuprofen sorption	1066:1083	Thermodynamic parameters for ibuprofen sorption were carried out and the ΔH and ΔS was found to be 76.23 & 0.233.					
36462695	2	23	theme	nanocomposites	324:337	arg1	synthesis					291:299	the green synthesis	281:299	the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal	281:432	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	2	23	theme	nanocomposites	324:337	arg1	aim					260:262	The aim	256:262	The aim of this study	256:276	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	11	24	theme	nanocomposites	1446:1459	arg1	group					1437:1441	a group	1435:1441	a group of nanocomposites	1435:1459	Fourier transform infrared spectroscopy spectra relate this band to a group of nanocomposites.					
36462695	11	24	theme	nanocomposites	1446:1459	arg1	nanocomposites					1446:1459	nanocomposites	1446:1459	nanocomposites	1446:1459	Fourier transform infrared spectroscopy spectra relate this band to a group of nanocomposites.					
36462695	0	25	theme	sorptive	26:33	arg1	elimination					35:45	sorptive elimination	26:45	sorptive elimination of ibuprofen from synthetic wastewater and its molecular docking studies	26:118	Porous nanocomposites for sorptive elimination of ibuprofen from synthetic wastewater and its molecular docking studies.					
36462695	2	26	dep	olifera	367:373	arg1	leaves					375:380	leaves	375:380	leaves extract and its applicability for ibuprofen removal	375:432	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	7	27	theme	Ibuprofen	895:903	arg1	elimination					905:915	Ibuprofen elimination	895:915	Ibuprofen elimination	895:915	Ibuprofen elimination was demonstrated to be most successful at a pH of 7.3, using 0.05 g of nanocomposites at a rotational speed of 200 rpm.					
36462695	7	28	from	pH	961:962	arg1	successful					945:954	successful	945:954	successful	945:954	Ibuprofen elimination was demonstrated to be most successful at a pH of 7.3, using 0.05 g of nanocomposites at a rotational speed of 200 rpm.					
36462695	10	29	theme	plasmon	1347:1353	arg1	resonances					1355:1364	the nanocomposite's surface plasmon resonances	1319:1364	the nanocomposite's surface plasmon resonances	1319:1364	UV-vis spectra confirm the 243 nm absorption band corresponding to the nanocomposite's surface plasmon resonances.					
36462695	1	30	theme	numerous	221:228	arg1	species					230:236	numerous species	221:236	numerous species in the ecosystem	221:253	Pharmaceuticals are a new developing pollutant that is threatening aquatic ecosystems and impacting numerous species in the ecosystem.					
36462695	3	31	theme	characterization	443:458	arg1	studies					460:466	Various characterization studies	435:466	Various characterization studies	435:466	Various characterization studies were performed for the synthesized nanocomposites.					
36462695	4	32	theme	composite	593:601	arg1	dosage					603:608	composite dosage	593:608	composite dosage	593:608	Box-Behnken design (BBD) is employed to optimize pH, agitation speed, and composite dosage.					
36462695	5	33	theme	pseudo-first-order	771:788	arg1	kinetics					790:797	pseudo-first-order kinetics	771:797	pseudo-first-order kinetics	771:797	Equilibrium results show that adsorption process matches with Langmuir isotherm, demonstrating adsorption on the nanocomposite's homogenous surface and follows pseudo-first-order kinetics.					
36462695	2	34	with	conjunction	342:352	arg1	olifera					367:373	olifera	367:373	olifera	367:373	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	10	35	theme	surface	1339:1345	arg1	resonances					1355:1364	the nanocomposite's surface plasmon resonances	1319:1364	the nanocomposite's surface plasmon resonances	1319:1364	UV-vis spectra confirm the 243 nm absorption band corresponding to the nanocomposite's surface plasmon resonances.					
36462695	3	36	theme	synthesized	491:501	arg1	nanocomposites					503:516	the synthesized nanocomposites	487:516	the synthesized nanocomposites	487:516	Various characterization studies were performed for the synthesized nanocomposites.					
36462695	0	37	from	wastewater	75:84	arg1	elimination					35:45	sorptive elimination	26:45	sorptive elimination of ibuprofen from synthetic wastewater and its molecular docking studies	26:118	Porous nanocomposites for sorptive elimination of ibuprofen from synthetic wastewater and its molecular docking studies.					
36462695	1	38	from	species	230:236	arg1	ecosystem					245:253	the ecosystem	241:253	the ecosystem	241:253	Pharmaceuticals are a new developing pollutant that is threatening aquatic ecosystems and impacting numerous species in the ecosystem.					
36462695	8	39	theme	76.23	1136:1140	arg1	ΔH					1110:1111	ΔH	1110:1111	ΔH	1110:1111	Thermodynamic parameters for ibuprofen sorption were carried out and the ΔH and ΔS was found to be 76.23 & 0.233.					
36462695	8	39	theme	76.23	1136:1140	arg1	0.233					1144:1148	76.23 & 0.233	1136:1148	76.23 & 0.233	1136:1148	Thermodynamic parameters for ibuprofen sorption were carried out and the ΔH and ΔS was found to be 76.23 & 0.233.					
36462695	9	40	theme	Molecular	1151:1159	arg1	Docking					1161:1167	Molecular Docking	1151:1167	Molecular Docking	1151:1167	Molecular Docking was performed to find the interaction between the pollutant and the nanocomposite.					
36462695	12	41	attach	removing	1555:1562	arg1	wastewater					1579:1588	wastewater	1579:1588	wastewater	1579:1588	The findings of this work emphasize the importance of TiO2-Fe2O3-Chitosan nanocomposites for removing ibuprofen from wastewater.					
36462695	12	41	attach	removing	1555:1562	arg2	importance					1502:1511	the importance	1498:1511	the importance of TiO2-Fe2O3-Chitosan nanocomposites for removing ibuprofen from wastewater	1498:1588	The findings of this work emphasize the importance of TiO2-Fe2O3-Chitosan nanocomposites for removing ibuprofen from wastewater.					
36462695	0	42	theme	ibuprofen	50:58	arg1	elimination					35:45	sorptive elimination	26:45	sorptive elimination of ibuprofen from synthetic wastewater and its molecular docking studies	26:118	Porous nanocomposites for sorptive elimination of ibuprofen from synthetic wastewater and its molecular docking studies.					
36462695	12	43	theme	nanocomposites	1536:1549	arg1	importance					1502:1511	the importance	1498:1511	the importance of TiO2-Fe2O3-Chitosan nanocomposites for removing ibuprofen from wastewater	1498:1588	The findings of this work emphasize the importance of TiO2-Fe2O3-Chitosan nanocomposites for removing ibuprofen from wastewater.					
36462695	4	44	theme	Box-Behnken	519:529	arg1	BBD					539:541	BBD	539:541	BBD	539:541	Box-Behnken design (BBD) is employed to optimize pH, agitation speed, and composite dosage.					
36462695	4	44	theme	Box-Behnken	519:529	arg1	design					531:536	Box-Behnken design	519:536	Box-Behnken design (BBD)	519:542	Box-Behnken design (BBD) is employed to optimize pH, agitation speed, and composite dosage.					
36462695	4	45	theme	agitation	572:580	arg1	speed					582:586	agitation speed	572:586	agitation speed	572:586	Box-Behnken design (BBD) is employed to optimize pH, agitation speed, and composite dosage.					
36462695	5	46	theme	homogenous	740:749	arg1	surface					751:757	the nanocomposite's homogenous surface	720:757	the nanocomposite's homogenous surface	720:757	Equilibrium results show that adsorption process matches with Langmuir isotherm, demonstrating adsorption on the nanocomposite's homogenous surface and follows pseudo-first-order kinetics.					
36462695	0	47	from	studies	112:118	arg1	elimination					35:45	sorptive elimination	26:45	sorptive elimination of ibuprofen from synthetic wastewater and its molecular docking studies	26:118	Porous nanocomposites for sorptive elimination of ibuprofen from synthetic wastewater and its molecular docking studies.					
36462695	2	48	theme	study	272:276	arg1	synthesis					291:299	the green synthesis	281:299	the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal	281:432	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	2	48	theme	study	272:276	arg1	aim					260:262	The aim	256:262	The aim of this study	256:276	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	5	49	theme	adsorption	641:650	arg1	process					652:658	adsorption process	641:658	adsorption process	641:658	Equilibrium results show that adsorption process matches with Langmuir isotherm, demonstrating adsorption on the nanocomposite's homogenous surface and follows pseudo-first-order kinetics.					
36462695	2	50	theme	TiO2-Fe2O3-Chitosan	304:322	arg1	nanocomposites					324:337	TiO2-Fe2O3-Chitosan nanocomposites	304:337	TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal	304:432	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	8	51	theme	&	1142:1142	arg1	ΔH					1110:1111	ΔH	1110:1111	ΔH	1110:1111	Thermodynamic parameters for ibuprofen sorption were carried out and the ΔH and ΔS was found to be 76.23 & 0.233.					
36462695	8	51	theme	&	1142:1142	arg1	0.233					1144:1148	76.23 & 0.233	1136:1148	76.23 & 0.233	1136:1148	Thermodynamic parameters for ibuprofen sorption were carried out and the ΔH and ΔS was found to be 76.23 & 0.233.					
36462695	6	52	theme	adsorbent	819:827	arg1	pH					815:816	pH	815:816	pH	815:816	Using the BBD, pH, adsorbent dose, and agitation speed were examined as adsorption parameters.					
36462695	6	52	theme	adsorbent	819:827	arg1	dose					829:832	adsorbent dose	819:832	adsorbent dose	819:832	Using the BBD, pH, adsorbent dose, and agitation speed were examined as adsorption parameters.					
36462695	6	52	theme	adsorbent	819:827	arg1	parameters					883:892	adsorption parameters	872:892	adsorption parameters	872:892	Using the BBD, pH, adsorbent dose, and agitation speed were examined as adsorption parameters.					
36462695	6	52	theme	adsorbent	819:827	arg1	speed					849:853	agitation speed	839:853	agitation speed	839:853	Using the BBD, pH, adsorbent dose, and agitation speed were examined as adsorption parameters.					
36462695	7	53	theme	200 rpm	1028:1034	arg1	speed					1019:1023	a rotational speed	1006:1023	a rotational speed of 200 rpm	1006:1034	Ibuprofen elimination was demonstrated to be most successful at a pH of 7.3, using 0.05 g of nanocomposites at a rotational speed of 200 rpm.					
36462695	1	54	theme	new	143:145	arg1	pollutant					158:166	a new developing pollutant	141:166	a new developing pollutant that is threatening aquatic ecosystems and impacting numerous species in the ecosystem	141:253	Pharmaceuticals are a new developing pollutant that is threatening aquatic ecosystems and impacting numerous species in the ecosystem.					
36462695	1	54	theme	new	143:145	arg1	Pharmaceuticals					121:135	Pharmaceuticals	121:135	Pharmaceuticals	121:135	Pharmaceuticals are a new developing pollutant that is threatening aquatic ecosystems and impacting numerous species in the ecosystem.					
36462695	2	55	from	synthesis	291:299	arg1	conjunction					342:352	conjunction	342:352	conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal	342:432	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	6	56	theme	adsorption	872:881	arg1	pH					815:816	pH	815:816	pH	815:816	Using the BBD, pH, adsorbent dose, and agitation speed were examined as adsorption parameters.					
36462695	6	56	theme	adsorption	872:881	arg1	dose					829:832	adsorbent dose	819:832	adsorbent dose	819:832	Using the BBD, pH, adsorbent dose, and agitation speed were examined as adsorption parameters.					
36462695	6	56	theme	adsorption	872:881	arg1	parameters					883:892	adsorption parameters	872:892	adsorption parameters	872:892	Using the BBD, pH, adsorbent dose, and agitation speed were examined as adsorption parameters.					
36462695	6	56	theme	adsorption	872:881	arg1	speed					849:853	agitation speed	839:853	agitation speed	839:853	Using the BBD, pH, adsorbent dose, and agitation speed were examined as adsorption parameters.					
36462695	1	57	theme	developing	147:156	arg1	pollutant					158:166	a new developing pollutant	141:166	a new developing pollutant that is threatening aquatic ecosystems and impacting numerous species in the ecosystem	141:253	Pharmaceuticals are a new developing pollutant that is threatening aquatic ecosystems and impacting numerous species in the ecosystem.					
36462695	1	57	theme	developing	147:156	arg1	Pharmaceuticals					121:135	Pharmaceuticals	121:135	Pharmaceuticals	121:135	Pharmaceuticals are a new developing pollutant that is threatening aquatic ecosystems and impacting numerous species in the ecosystem.					
36462695	2	58	theme	ibuprofen	416:424	arg1	removal					426:432	ibuprofen removal	416:432	ibuprofen removal	416:432	The aim of this study is the green synthesis of TiO2-Fe2O3-Chitosan nanocomposites in conjunction with Moringa olifera leaves extract and its applicability for ibuprofen removal.					
36462695	0	59	theme	synthetic	65:73	arg1	wastewater					75:84	synthetic wastewater	65:84	synthetic wastewater	65:84	Porous nanocomposites for sorptive elimination of ibuprofen from synthetic wastewater and its molecular docking studies.					
36928367	5	0	theme	neural	740:745	arg1	ANN					756:758	ANN	756:758	ANN	756:758	The artificial neural network (ANN) was employed for analyzing, validating, and predicting CNPs biosynthesis using Olea europaea leaves extract.					
36928367	5	0	theme	neural	740:745	arg1	network					747:753	The artificial neural network	725:753	The artificial neural network (ANN)	725:759	The artificial neural network (ANN) was employed for analyzing, validating, and predicting CNPs biosynthesis using Olea europaea leaves extract.					
36928367	3	1	theme	Olea	579:582	arg1	europaea					584:591	Olea europaea	579:591	Olea europaea leaves extract	579:606	The current study describes an alternative, biologically-based strategy for CNPs biosynthesis using Olea europaea leaves extract.					
36928367	2	2	contain	have	309:312	arg2	range					321:325	a wide range	314:325	a wide range of applications	314:341	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	2	2	contain	have	309:312	arg1	CNPs					304:307	CNPs	304:307	CNPs	304:307	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	5	3	theme	artificial	729:738	arg1	ANN					756:758	ANN	756:758	ANN	756:758	The artificial neural network (ANN) was employed for analyzing, validating, and predicting CNPs biosynthesis using Olea europaea leaves extract.					
36928367	5	3	theme	artificial	729:738	arg1	network					747:753	The artificial neural network	725:753	The artificial neural network (ANN)	725:759	The artificial neural network (ANN) was employed for analyzing, validating, and predicting CNPs biosynthesis using Olea europaea leaves extract.					
36928367	10	4	theme	CNPs	1400:1403	arg1	surface					1385:1391	The surface	1381:1391	The surface of the CNPs	1381:1403	The surface of the CNPs is positively charged, having a Zeta potential of 33.1 mV.					
36928367	10	4	theme	CNPs	1400:1403	arg1	charged					1419:1425	charged	1419:1425	charged	1419:1425	The surface of the CNPs is positively charged, having a Zeta potential of 33.1 mV.					
36928367	4	5	with	design	641:646	arg1	experiments					665:675	50 experiments	662:675	50 experiments	662:675	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	15	6	dep	application	2212:2222	arg1	addition					2184:2191	addition	2184:2191	addition	2184:2191	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	5	7	dep	europaea	845:852	arg1	leaves					854:859	leaves	854:859	leaves extract	854:867	The artificial neural network (ANN) was employed for analyzing, validating, and predicting CNPs biosynthesis using Olea europaea leaves extract.					
36928367	5	8	theme	Olea	840:843	arg1	europaea					845:852	Olea europaea	840:852	Olea europaea leaves extract	840:867	The artificial neural network (ANN) was employed for analyzing, validating, and predicting CNPs biosynthesis using Olea europaea leaves extract.					
36928367	7	9	theme	initial	1150:1156	arg1	pH					1158:1159	initial pH 4.47	1150:1164	initial pH 4.47	1150:1164	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	15	10	theme	medical	2142:2148	arg1	industries					2169:2178	the medical and pharmaceutical industries	2138:2178	the medical and pharmaceutical industries	2138:2178	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	6	11	theme	CNPs	938:941	arg1	biosynthesis					943:954	maximum CNPs biosynthesis	930:954	maximum CNPs biosynthesis	930:954	Using the desirability function, the optimum conditions for maximum CNPs biosynthesis were determined theoretically and verified experimentally.					
36928367	7	12	theme	%	1147:1147	arg1	solution					1132:1139	leaves extract solution	1117:1139	leaves extract solution of 100%	1117:1147	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	7	12	theme	%	1147:1147	arg1	time					1182:1185	incubation time	1171:1185	incubation time of 60 min	1171:1195	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	7	12	theme	%	1147:1147	arg1	pH					1158:1159	initial pH 4.47	1150:1164	initial pH 4.47	1150:1164	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	7	12	theme	%	1147:1147	arg1	solution					1101:1108	chitosan solution	1092:1108	chitosan solution of 1%	1092:1114	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	2	13	theme	unique	356:361	arg1	antimicrobials					444:457	antimicrobials	444:457	antimicrobials	444:457	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	2	13	theme	unique	356:361	arg1	characteristics					363:377	their unique characteristics	350:377	their unique characteristics	350:377	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	2	13	theme	unique	356:361	arg1	protection					417:426	protection	417:426	protection	417:426	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	2	13	theme	unique	356:361	arg1	encapsulation					464:476	encapsulation	464:476	encapsulation	464:476	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	2	13	theme	unique	356:361	arg1	growth					396:401	plant growth promotion and protection	390:426	growth	396:401	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	2	13	theme	unique	356:361	arg1	delivery					434:441	drug delivery	429:441	drug delivery	429:441	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	1	14	theme	excellent	235:243	arg1	properties					292:301	excellent physicochemical, antimicrobial, and biological properties	235:301	excellent physicochemical, antimicrobial, and biological properties	235:301	Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties.					
36928367	4	15	used	used	681:684	arg2	FCCCD					649:653	FCCCD	649:653	FCCCD	649:653	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	4	15	used	used	681:684	arg2	design					641:646	Face centered central composite design	609:646	Face centered central composite design (FCCCD)	609:654	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	2	16	theme	drug	429:432	arg1	delivery					434:441	drug delivery	429:441	drug delivery	429:441	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	4	17	theme	CNPs	706:709	arg1	biosynthesis					711:722	CNPs biosynthesis	706:722	CNPs biosynthesis	706:722	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	7	18	theme	extract	1124:1130	arg1	solution					1132:1139	leaves extract solution	1117:1139	leaves extract solution of 100%	1117:1147	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	8	19	contain	had	1251:1253	arg1	CNPs					1246:1249	CNPs	1246:1249	CNPs	1246:1249	The SEM and TEM images revealed that CNPs had a spherical form and varied in size between 6.91 and 11.14 nm.					
36928367	8	19	contain	had	1251:1253	arg2	form					1267:1270	a spherical form	1255:1270	a spherical form	1255:1270	The SEM and TEM images revealed that CNPs had a spherical form and varied in size between 6.91 and 11.14 nm.					
36928367	7	20	theme	chitosan	1092:1099	arg1	solution					1101:1108	chitosan solution	1092:1108	chitosan solution of 1%	1092:1114	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	14	21	theme	metabolic	1875:1883	arg1	activity					1885:1892	metabolic activity	1875:1892	metabolic activity	1875:1892	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	1	22	theme	Chitosan	159:166	arg1	nanoparticles					168:180	Chitosan nanoparticles	159:180	Chitosan nanoparticles (CNPs)	159:187	Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties.					
36928367	1	22	theme	Chitosan	159:166	arg1	nanoparticles					216:228	promising biopolymeric nanoparticles	193:228	promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties	193:301	Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties.					
36928367	1	22	theme	Chitosan	159:166	arg1	CNPs					183:186	CNPs	183:186	CNPs	183:186	Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties.					
36928367	2	23	theme	wide	316:319	arg1	range					321:325	a wide range	314:325	a wide range of applications	314:341	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	11	24	theme	functional	1495:1504	arg1	C-O					1528:1530	C-O	1528:1530	C-O	1528:1530	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	11	24	theme	functional	1495:1504	arg1	C-OH					1545:1548	C-OH	1545:1548	C-OH	1545:1548	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	11	24	theme	functional	1495:1504	arg1	C-O-C					1554:1558	C-O-C	1554:1558	C-O-C	1554:1558	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	11	24	theme	functional	1495:1504	arg1	groups					1506:1511	various functional groups	1487:1511	various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C	1487:1558	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	11	24	theme	functional	1495:1504	arg1	CONH2					1533:1537	CONH2	1533:1537	CONH2	1533:1537	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	11	24	theme	functional	1495:1504	arg1	NH2					1540:1542	NH2	1540:1542	NH2	1540:1542	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	11	24	theme	functional	1495:1504	arg1	C-H					1523:1525	C-H	1523:1525	C-H	1523:1525	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	7	25	theme	21.15 mg	1055:1062	arg1	CNPs					1049:1052	CNPs	1049:1052	CNPs (21.15 mg CNPs/mL)	1049:1071	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	7	25	theme	21.15 mg	1055:1062	arg1	CNPs/mL					1064:1070	21.15 mg CNPs/mL	1055:1070	21.15 mg CNPs/mL	1055:1070	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	14	26	theme	formation	1830:1838	arg1	Inhibition					1808:1817	Inhibition	1808:1817	Inhibition of biofilm formation	1808:1838	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	10	27	theme	33.1 mV	1455:1461	arg1	potential					1442:1450	a Zeta potential	1435:1450	a Zeta potential of 33.1 mV	1435:1461	The surface of the CNPs is positively charged, having a Zeta potential of 33.1 mV.					
36928367	7	28	theme	CNPs	1049:1052	arg1	yield					1040:1044	The highest experimental yield	1015:1044	The highest experimental yield of CNPs (21.15 mg CNPs/mL)	1015:1071	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	1	29	theme	promising	193:201	arg1	nanoparticles					168:180	Chitosan nanoparticles	159:180	Chitosan nanoparticles (CNPs)	159:187	Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties.					
36928367	1	29	theme	promising	193:201	arg1	nanoparticles					216:228	promising biopolymeric nanoparticles	193:228	promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties	193:301	Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties.					
36928367	9	30	theme	X-ray	1318:1322	arg1	diffraction					1324:1334	X-ray diffraction	1318:1334	X-ray diffraction	1318:1334	X-ray diffraction demonstrates the crystalline nature of CNPs.					
36928367	2	31	theme	applications	330:341	arg1	range					321:325	a wide range	314:325	a wide range of applications	314:341	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	13	32	theme	biofilm	1670:1676	arg1	formation					1678:1686	biofilm formation	1670:1686	biofilm formation	1670:1686	The CNPs were able to suppress biofilm formation by P. aeruginosa, S. aureus and C. albicans at concentrations ranging from 10 to 1500 µg/mL in a dose-dependent manner.					
36928367	0	33	theme	anti-biofilm	132:143	arg1	potentiality					145:156	its anti-biofilm potentiality	128:156	its anti-biofilm potentiality	128:156	Artificial intelligence-based optimization for chitosan nanoparticles biosynthesis, characterization and in‑vitro assessment of its anti-biofilm potentiality.					
36928367	13	34	from	concentrations	1735:1748	arg1	able					1653:1656	able	1653:1656	able	1653:1656	The CNPs were able to suppress biofilm formation by P. aeruginosa, S. aureus and C. albicans at concentrations ranging from 10 to 1500 µg/mL in a dose-dependent manner.					
36928367	13	34	from	concentrations	1735:1748	arg1	CNPs					1643:1646	The CNPs	1639:1646	The CNPs	1639:1646	The CNPs were able to suppress biofilm formation by P. aeruginosa, S. aureus and C. albicans at concentrations ranging from 10 to 1500 µg/mL in a dose-dependent manner.					
36928367	7	35	theme	highest	1019:1025	arg1	yield					1040:1044	The highest experimental yield	1015:1044	The highest experimental yield of CNPs (21.15 mg CNPs/mL)	1015:1071	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	0	36	theme	Artificial	0:9	arg1	optimization					30:41	Artificial intelligence-based optimization	0:41	Artificial intelligence-based optimization for chitosan	0:54	Artificial intelligence-based optimization for chitosan nanoparticles biosynthesis, characterization and in‑vitro assessment of its anti-biofilm potentiality.					
36928367	9	37	theme	crystalline	1353:1363	arg1	nature					1365:1370	the crystalline nature	1349:1370	the crystalline nature of CNPs	1349:1378	X-ray diffraction demonstrates the crystalline nature of CNPs.					
36928367	4	38	theme	central	623:629	arg1	FCCCD					649:653	FCCCD	649:653	FCCCD	649:653	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	4	38	theme	central	623:629	arg1	design					641:646	Face centered central composite design	609:646	Face centered central composite design (FCCCD)	609:654	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	2	39	dep	growth	396:401	arg1	promotion					403:411	promotion	403:411	promotion	403:411	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	15	40	theme	multidrug-resistant	2239:2257	arg1	microorganisms					2259:2272	multidrug-resistant microorganisms	2239:2272	multidrug-resistant microorganisms	2239:2272	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	6	41	theme	maximum	930:936	arg1	biosynthesis					943:954	maximum CNPs biosynthesis	930:954	maximum CNPs biosynthesis	930:954	Using the desirability function, the optimum conditions for maximum CNPs biosynthesis were determined theoretically and verified experimentally.					
36928367	11	42	theme	FTIR	1464:1467	arg1	analysis					1469:1476	FTIR analysis	1464:1476	FTIR analysis	1464:1476	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	1	43	theme	biopolymeric	203:214	arg1	nanoparticles					168:180	Chitosan nanoparticles	159:180	Chitosan nanoparticles (CNPs)	159:187	Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties.					
36928367	1	43	theme	biopolymeric	203:214	arg1	nanoparticles					216:228	promising biopolymeric nanoparticles	193:228	promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties	193:301	Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties.					
36928367	7	44	theme	60 min	1190:1195	arg1	solution					1132:1139	leaves extract solution	1117:1139	leaves extract solution of 100%	1117:1147	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	7	44	theme	60 min	1190:1195	arg1	time					1182:1185	incubation time	1171:1185	incubation time of 60 min	1171:1195	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	7	44	theme	60 min	1190:1195	arg1	pH					1158:1159	initial pH 4.47	1150:1164	initial pH 4.47	1150:1164	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	7	44	theme	60 min	1190:1195	arg1	solution					1101:1108	chitosan solution	1092:1108	chitosan solution of 1%	1092:1114	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	0	45	dep	biosynthesis	70:81	arg1	assessment					114:123	assessment	114:123	assessment	114:123	Artificial intelligence-based optimization for chitosan nanoparticles biosynthesis, characterization and in‑vitro assessment of its anti-biofilm potentiality.					
36928367	3	46	theme	CNPs	555:558	arg1	biosynthesis					560:571	CNPs biosynthesis	555:571	CNPs biosynthesis using Olea europaea leaves extract	555:606	The current study describes an alternative, biologically-based strategy for CNPs biosynthesis using Olea europaea leaves extract.					
36928367	15	47	theme	potential	2202:2210	arg1	those					2286:2290	those	2286:2290	those	2286:2290	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	15	47	theme	potential	2202:2210	arg1	application					2212:2222	their potential application	2196:2222	their potential application in controlling multidrug-resistant microorganisms	2196:2272	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	15	48	theme	Olea	2075:2078	arg1	europaea					2080:2087	Olea europaea	2075:2087	Olea europaea leaves extract	2075:2102	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	4	49	theme	Face	609:612	arg1	FCCCD					649:653	FCCCD	649:653	FCCCD	649:653	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	4	49	theme	Face	609:612	arg1	design					641:646	Face centered central composite design	609:646	Face centered central composite design (FCCCD)	609:654	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	1	50	with	nanoparticles	216:228	arg1	properties					292:301	excellent physicochemical, antimicrobial, and biological properties	235:301	excellent physicochemical, antimicrobial, and biological properties	235:301	Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties.					
36928367	14	51	theme	encased	1961:1967	arg1	cells					1969:1973	biofilm encased cells	1953:1973	biofilm encased cells	1953:1973	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	13	52	theme	dose-dependent	1785:1798	arg1	manner					1800:1805	a dose-dependent manner	1783:1805	a dose-dependent manner	1783:1805	The CNPs were able to suppress biofilm formation by P. aeruginosa, S. aureus and C. albicans at concentrations ranging from 10 to 1500 µg/mL in a dose-dependent manner.					
36928367	15	53	theme	COVID-19	2313:2320	arg1	pneumonia					2322:2330	post COVID-19 pneumonia	2308:2330	post COVID-19 pneumonia in immunosuppressed patients	2308:2359	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	15	54	from	range	2028:2032	arg1	promising					2108:2116	promising	2108:2116	promising	2108:2116	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	7	55	dep	solution	1132:1139	arg1	leaves					1117:1122	leaves	1117:1122	leaves	1117:1122	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	14	56	theme	hydrophobicity	1935:1948	arg1	suppression					1860:1870	suppression	1860:1870	suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells	1860:1973	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	4	57	theme	biosynthesis	711:722	arg1	optimization					690:701	optimization	690:701	optimization of CNPs biosynthesis	690:722	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	8	58	theme	SEM	1213:1215	arg1	images					1225:1230	The SEM and TEM images	1209:1230	The SEM and TEM images	1209:1230	The SEM and TEM images revealed that CNPs had a spherical form and varied in size between 6.91 and 11.14 nm.					
36928367	1	59	theme	physicochemical	245:259	arg1	properties					292:301	excellent physicochemical, antimicrobial, and biological properties	235:301	excellent physicochemical, antimicrobial, and biological properties	235:301	Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties.					
36928367	8	60	theme	TEM	1221:1223	arg1	images					1225:1230	The SEM and TEM images	1209:1230	The SEM and TEM images	1209:1230	The SEM and TEM images revealed that CNPs had a spherical form and varied in size between 6.91 and 11.14 nm.					
36928367	15	61	theme	pharmaceutical	2154:2167	arg1	industries					2169:2178	the medical and pharmaceutical industries	2138:2178	the medical and pharmaceutical industries	2138:2178	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	14	62	theme	moieties	1921:1928	arg1	suppression					1860:1870	suppression	1860:1870	suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells	1860:1973	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	14	63	theme	activity	1885:1892	arg1	suppression					1860:1870	suppression	1860:1870	suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells	1860:1973	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	12	64	theme	thermogravimetric	1565:1581	arg1	investigation					1583:1595	The thermogravimetric investigation	1561:1595	The thermogravimetric investigation	1561:1595	The thermogravimetric investigation indicated that CNPs are thermally stable.					
36928367	7	65	theme	%	1114:1114	arg1	solution					1132:1139	leaves extract solution	1117:1139	leaves extract solution of 100%	1117:1147	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	7	65	theme	%	1114:1114	arg1	time					1182:1185	incubation time	1171:1185	incubation time of 60 min	1171:1195	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	7	65	theme	%	1114:1114	arg1	pH					1158:1159	initial pH 4.47	1150:1164	initial pH 4.47	1150:1164	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	7	65	theme	%	1114:1114	arg1	solution					1101:1108	chitosan solution	1092:1108	chitosan solution of 1%	1092:1114	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	15	66	from	pneumonia	2322:2330	arg1	patients					2352:2359	immunosuppressed patients	2335:2359	immunosuppressed patients	2335:2359	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	15	67	from	promising	2108:2116	arg1	range					2028:2032	the range	2024:2032	the range of 6.91 to 11.14 nm	2024:2052	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	14	68	dep	r	1976:1976	arg1	˃					1978:1978	˃ 0.9	1978:1982	r ˃ 0.9	1976:1982	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	14	68	dep	r	1976:1976	arg1	P = 0.00					1985:1992	P = 0.00	1985:1992	P = 0.00	1985:1992	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	15	69	from	applications	2122:2133	arg1	industries					2169:2178	the medical and pharmaceutical industries	2138:2178	the medical and pharmaceutical industries	2138:2178	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	11	70	theme	various	1487:1493	arg1	C-O					1528:1530	C-O	1528:1530	C-O	1528:1530	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	11	70	theme	various	1487:1493	arg1	C-OH					1545:1548	C-OH	1545:1548	C-OH	1545:1548	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	11	70	theme	various	1487:1493	arg1	C-O-C					1554:1558	C-O-C	1554:1558	C-O-C	1554:1558	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	11	70	theme	various	1487:1493	arg1	groups					1506:1511	various functional groups	1487:1511	various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C	1487:1558	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	11	70	theme	various	1487:1493	arg1	CONH2					1533:1537	CONH2	1533:1537	CONH2	1533:1537	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	11	70	theme	various	1487:1493	arg1	NH2					1540:1542	NH2	1540:1542	NH2	1540:1542	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	11	70	theme	various	1487:1493	arg1	C-H					1523:1525	C-H	1523:1525	C-H	1523:1525	FTIR analysis revealed various functional groups including C-H, C-O, CONH2, NH2, C-OH and C-O-C.					
36928367	14	71	theme	protein/exopolysaccharide	1895:1919	arg1	moieties					1921:1928	protein/exopolysaccharide moieties	1895:1928	protein/exopolysaccharide moieties	1895:1928	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	15	72	theme	post	2308:2311	arg1	pneumonia					2322:2330	post COVID-19 pneumonia	2308:2330	post COVID-19 pneumonia in immunosuppressed patients	2308:2359	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	5	73	theme	CNPs	816:819	arg1	biosynthesis					821:832	CNPs biosynthesis	816:832	CNPs biosynthesis using Olea europaea leaves extract	816:867	The artificial neural network (ANN) was employed for analyzing, validating, and predicting CNPs biosynthesis using Olea europaea leaves extract.					
36928367	14	74	theme	biofilm	1822:1828	arg1	formation					1830:1838	biofilm formation	1822:1838	biofilm formation	1822:1838	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	3	75	dep	alternative	510:520	arg1	biologically-based					523:540	biologically-based	523:540	biologically-based	523:540	The current study describes an alternative, biologically-based strategy for CNPs biosynthesis using Olea europaea leaves extract.					
36928367	10	76	theme	Zeta	1437:1440	arg1	potential					1442:1450	a Zeta potential	1435:1450	a Zeta potential of 33.1 mV	1435:1461	The surface of the CNPs is positively charged, having a Zeta potential of 33.1 mV.					
36928367	0	77	theme	potentiality	145:156	arg1	in‑vitro					105:112	in‑vitro	105:112	in‑vitro	105:112	Artificial intelligence-based optimization for chitosan nanoparticles biosynthesis, characterization and in‑vitro assessment of its anti-biofilm potentiality.					
36928367	0	77	theme	potentiality	145:156	arg1	characterization					84:99	characterization	84:99	characterization	84:99	Artificial intelligence-based optimization for chitosan nanoparticles biosynthesis, characterization and in‑vitro assessment of its anti-biofilm potentiality.					
36928367	0	77	theme	potentiality	145:156	arg1	biosynthesis					70:81	biosynthesis	70:81	biosynthesis	70:81	Artificial intelligence-based optimization for chitosan nanoparticles biosynthesis, characterization and in‑vitro assessment of its anti-biofilm potentiality.					
36928367	15	78	theme	immunosuppressed	2335:2350	arg1	patients					2352:2359	immunosuppressed patients	2335:2359	immunosuppressed patients	2335:2359	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	0	79	theme	intelligence-based	11:28	arg1	optimization					30:41	Artificial intelligence-based optimization	0:41	Artificial intelligence-based optimization for chitosan	0:54	Artificial intelligence-based optimization for chitosan nanoparticles biosynthesis, characterization and in‑vitro assessment of its anti-biofilm potentiality.					
36928367	7	80	theme	experimental	1027:1038	arg1	yield					1040:1044	The highest experimental yield	1015:1044	The highest experimental yield of CNPs (21.15 mg CNPs/mL)	1015:1071	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	15	81	dep	europaea	2080:2087	arg1	leaves					2089:2094	leaves	2089:2094	leaves extract	2089:2102	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	4	82	theme	composite	631:639	arg1	FCCCD					649:653	FCCCD	649:653	FCCCD	649:653	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	4	82	theme	composite	631:639	arg1	design					641:646	Face centered central composite design	609:646	Face centered central composite design (FCCCD)	609:654	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	8	83	theme	spherical	1257:1265	arg1	form					1267:1270	a spherical form	1255:1270	a spherical form	1255:1270	The SEM and TEM images revealed that CNPs had a spherical form and varied in size between 6.91 and 11.14 nm.					
36928367	3	84	theme	current	483:489	arg1	study					491:495	The current study	479:495	The current study	479:495	The current study describes an alternative, biologically-based strategy for CNPs biosynthesis using Olea europaea leaves extract.					
36928367	15	85	theme	11.14 nm	2045:2052	arg1	range					2028:2032	the range	2024:2032	the range of 6.91 to 11.14 nm	2024:2052	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	3	86	dep	europaea	584:591	arg1	leaves					593:598	leaves	593:598	leaves extract	593:606	The current study describes an alternative, biologically-based strategy for CNPs biosynthesis using Olea europaea leaves extract.					
36928367	15	87	dep	11.14 nm	2045:2052	arg1	to					2042:2043	to	2042:2043	to	2042:2043	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	15	88	theme	small	2009:2013	arg1	size					2015:2018	their small size	2003:2018	their small size	2003:2018	Due to their small size, in the range of 6.91 to 11.14 nm, CNPs produced using Olea europaea leaves extract are promising for applications in the medical and pharmaceutical industries, in addition to their potential application in controlling multidrug-resistant microorganisms, especially those associated with post COVID-19 pneumonia in immunosuppressed patients.					
36928367	9	89	theme	CNPs	1375:1378	arg1	nature					1365:1370	the crystalline nature	1349:1370	the crystalline nature of CNPs	1349:1378	X-ray diffraction demonstrates the crystalline nature of CNPs.					
36928367	1	90	theme	antimicrobial	262:274	arg1	properties					292:301	excellent physicochemical, antimicrobial, and biological properties	235:301	excellent physicochemical, antimicrobial, and biological properties	235:301	Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties.					
36928367	3	91	theme	alternative	510:520	arg1	strategy					542:549	an alternative, biologically-based strategy	507:549	an alternative, biologically-based strategy for CNPs biosynthesis using Olea europaea leaves extract	507:606	The current study describes an alternative, biologically-based strategy for CNPs biosynthesis using Olea europaea leaves extract.					
36928367	7	92	theme	incubation	1171:1180	arg1	time					1182:1185	incubation time	1171:1185	incubation time of 60 min	1171:1195	The highest experimental yield of CNPs (21.15 mg CNPs/mL) was obtained using chitosan solution of 1%, leaves extract solution of 100%, initial pH 4.47, and incubation time of 60 min at 53.83°C.					
36928367	6	93	theme	optimum	907:913	arg1	conditions					915:924	the optimum conditions	903:924	the optimum conditions for maximum CNPs biosynthesis	903:954	Using the desirability function, the optimum conditions for maximum CNPs biosynthesis were determined theoretically and verified experimentally.					
36928367	4	94	theme	centered	614:621	arg1	FCCCD					649:653	FCCCD	649:653	FCCCD	649:653	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	4	94	theme	centered	614:621	arg1	design					641:646	Face centered central composite design	609:646	Face centered central composite design (FCCCD)	609:654	Face centered central composite design (FCCCD), with 50 experiments was used for optimization of CNPs biosynthesis.					
36928367	2	95	theme	plant	390:394	arg1	growth					396:401	plant growth promotion and protection	390:426	growth	396:401	CNPs have a wide range of applications due to their unique characteristics, including plant growth promotion and protection, drug delivery, antimicrobials, and encapsulation.					
36928367	14	96	theme	cells	1969:1973	arg1	activity					1885:1892	metabolic activity	1875:1892	metabolic activity	1875:1892	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	14	96	theme	cells	1969:1973	arg1	moieties					1921:1928	protein/exopolysaccharide moieties	1895:1928	protein/exopolysaccharide moieties	1895:1928	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	14	96	theme	cells	1969:1973	arg1	hydrophobicity					1935:1948	hydrophobicity	1935:1948	hydrophobicity of biofilm encased cells	1935:1973	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	1	97	theme	biological	281:290	arg1	properties					292:301	excellent physicochemical, antimicrobial, and biological properties	235:301	excellent physicochemical, antimicrobial, and biological properties	235:301	Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties.					
36928367	6	98	theme	desirability	880:891	arg1	function					893:900	the desirability function	876:900	the desirability function	876:900	Using the desirability function, the optimum conditions for maximum CNPs biosynthesis were determined theoretically and verified experimentally.					
36928367	14	99	theme	biofilm	1953:1959	arg1	cells					1969:1973	biofilm encased cells	1953:1973	biofilm encased cells	1953:1973	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36928367	14	100	dep	associated	1844:1853	arg1	r					1976:1976	r	1976:1976	r ˃ 0.9	1976:1982	Inhibition of biofilm formation was associated with suppression of metabolic activity, protein/exopolysaccharide moieties, and hydrophobicity of biofilm encased cells (r ˃ 0.9, P = 0.00).					
36053424	8	0	theme	emission	1705:1712	arg1	technologies					1722:1733	emission control technologies	1705:1733	emission control technologies	1705:1733	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	4	1	theme	particle	991:998	arg1	collection					1000:1009	fine particle collection	986:1009	fine particle collection	986:1009	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	8	2	with	electricity	1661:1671	arg1	burning					1696:1702	sugarcane bagasse burning	1678:1702	sugarcane bagasse burning	1678:1702	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	4	3	theme	emerging	752:759	arg1	use					707:709	the appropriate use	691:709	the appropriate use of biomass	691:720	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	4	3	theme	emerging	752:759	arg1	need					761:764	the emerging need	748:764	the emerging need for a technique capable of efficiently removing pollutants from biomass burning	748:844	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	4	4	theme	capable	782:788	arg1	technique					772:780	a technique	770:780	a technique capable of efficiently removing pollutants from biomass burning	770:844	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	8	5	theme	cost-effective	1739:1752	arg1	samplers					1777:1784	cost-effective and efficient portable samplers	1739:1784	cost-effective and efficient portable samplers	1739:1784	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	5	6	theme	current	1306:1312	arg1	situation					1314:1322	the current situation	1302:1322	the current situation of concern about air quality	1302:1351	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	1	7	theme	biomass	175:181	arg1	burning					183:189	biomass burning	175:189	biomass burning	175:189	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	4	8	theme	Venturi	966:972	arg1	scrubber					974:981	a Venturi scrubber	964:981	a Venturi scrubber	964:981	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	8	9	theme	efficient	1758:1766	arg1	samplers					1777:1784	cost-effective and efficient portable samplers	1739:1784	cost-effective and efficient portable samplers	1739:1784	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	1	10	from	one	194:196	arg1	countries					254:262	countries	254:262	countries	254:262	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	1	10	from	one	194:196	arg1	Brazil					316:321	Brazil	316:321	Brazil	316:321	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	7	11	theme	ionic	1467:1471	arg1	analysis					1473:1480	The ionic analysis	1463:1480	The ionic analysis for PM< 1.0 filters	1463:1500	The ionic analysis for PM< 1.0 filters showed potassium, chloride, nitrate, and nitrite at concentrations ranging from 20.12 to 36.5 μg/m3.					
36053424	3	12	theme	PM	667:668	arg1	composition					648:658	the composition	644:658	the composition of the PM	644:668	Controlling these emissions is necessary; therefore, the aim was to evaluate PM collection using a rectangular Venturi scrubber (RVS), and its effects on the composition of the PM emitted.					
36053424	4	13	theme	isokinetic	1061:1070	arg1	equipment					1081:1089	portable and representative isokinetic sampling equipment	1033:1089	portable and representative isokinetic sampling equipment of these particles	1033:1108	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	5	14	theme	representative	1174:1187	arg1	sampling					1189:1196	the representative sampling	1170:1196	the representative sampling of fine particles and obtaining parameters	1170:1239	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	4	15	theme	emissions	879:887	arg1	innovation					895:904	an innovation	892:904	an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection	892:1009	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	4	15	theme	emissions	879:887	arg1	control					868:874	the control	864:874	the control of emissions	864:887	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	0	16	theme	matter	66:71	arg1	collection					73:82	particulate matter collection	54:82	particulate matter collection	54:82	Evaluation of the efficiency of a Venturi scrubber in particulate matter collection smaller than 2.5 µm emitted by biomass burning.					
36053424	1	17	theme	increased	150:158	arg1	worldwide					160:168	increased worldwide	150:168	increased worldwide	150:168	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	5	18	theme	burning	1153:1159	arg1	process					1161:1167	the biomass burning process	1141:1167	the biomass burning process	1141:1167	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	8	19	theme	gas	1850:1852	arg1	projects					1873:1880	current gas cleaning equipment projects	1842:1880	current gas cleaning equipment projects	1842:1880	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	2	20	theme	sugarcane	458:466	arg1	burning					481:487	sugarcane bagasse (SB) burning	458:487	sugarcane bagasse (SB) burning	458:487	However, these energy sources generate pollutants, consisting of particulate matter (PM) with a complex chemical composition, such as sugarcane bagasse (SB) burning.					
36053424	5	21	theme	obtaining	1220:1228	arg1	parameters					1230:1239	obtaining parameters	1220:1239	obtaining parameters	1220:1239	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	8	22	theme	equipment	1863:1871	arg1	projects					1873:1880	current gas cleaning equipment projects	1842:1880	current gas cleaning equipment projects	1842:1880	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	4	23	with	one	944:946	arg1	use					957:959	the use	953:959	the use of a Venturi scrubber in fine particle collection	953:1009	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	0	24	dep	smaller	84:90	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the efficiency of a Venturi scrubber in particulate matter collection	0:82	Evaluation of the efficiency of a Venturi scrubber in particulate matter collection smaller than 2.5 µm emitted by biomass burning.					
36053424	2	25	theme	energy	339:344	arg1	sources					346:352	these energy sources	333:352	these energy sources	333:352	However, these energy sources generate pollutants, consisting of particulate matter (PM) with a complex chemical composition, such as sugarcane bagasse (SB) burning.					
36053424	8	26	theme	bagasse	1688:1694	arg1	burning					1696:1702	sugarcane bagasse burning	1678:1702	sugarcane bagasse burning	1678:1702	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	2	27	theme	complex	420:426	arg1	composition					437:447	a complex chemical composition	418:447	a complex chemical composition	418:447	However, these energy sources generate pollutants, consisting of particulate matter (PM) with a complex chemical composition, such as sugarcane bagasse (SB) burning.					
36053424	5	28	theme	particles	1206:1214	arg1	sampling					1189:1196	the representative sampling	1170:1196	the representative sampling of fine particles and obtaining parameters	1170:1239	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	3	29	theme	PM	567:568	arg1	collection					570:579	PM collection	567:579	PM collection	567:579	Controlling these emissions is necessary; therefore, the aim was to evaluate PM collection using a rectangular Venturi scrubber (RVS), and its effects on the composition of the PM emitted.					
36053424	4	30	theme	industrial	728:737	arg1	use					707:709	the appropriate use	691:709	the appropriate use of biomass	691:720	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	4	30	theme	industrial	728:737	arg1	fuel					739:742	an industrial fuel	725:742	an industrial fuel	725:742	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	5	31	theme	Venturi	1278:1284	arg1	scrubber					1286:1293	a Venturi scrubber	1276:1293	a Venturi scrubber	1276:1293	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	0	32	theme	Venturi	34:40	arg1	scrubber					42:49	a Venturi scrubber	32:49	a Venturi scrubber in particulate matter collection	32:82	Evaluation of the efficiency of a Venturi scrubber in particulate matter collection smaller than 2.5 µm emitted by biomass burning.					
36053424	4	33	theme	appropriate	695:705	arg1	use					707:709	the appropriate use	691:709	the appropriate use of biomass	691:720	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	4	33	theme	appropriate	695:705	arg1	fuel					739:742	an industrial fuel	725:742	an industrial fuel	725:742	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	4	33	theme	appropriate	695:705	arg1	need					761:764	the emerging need	748:764	the emerging need for a technique capable of efficiently removing pollutants from biomass burning	748:844	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	3	34	theme	rectangular	589:599	arg1	RVS					619:621	RVS	619:621	RVS	619:621	Controlling these emissions is necessary; therefore, the aim was to evaluate PM collection using a rectangular Venturi scrubber (RVS), and its effects on the composition of the PM emitted.					
36053424	3	34	theme	rectangular	589:599	arg1	scrubber					609:616	a rectangular Venturi scrubber	587:616	a rectangular Venturi scrubber (RVS)	587:622	Controlling these emissions is necessary; therefore, the aim was to evaluate PM collection using a rectangular Venturi scrubber (RVS), and its effects on the composition of the PM emitted.					
36053424	5	35	theme	pollutant	1252:1260	arg1	emissions					1262:1270	pollutant emissions	1252:1270	pollutant emissions for a Venturi scrubber	1252:1293	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	6	36	theme	collection	1366:1375	arg1	values					1388:1393	The average collection efficiency values	1354:1393	The average collection efficiency values	1354:1393	The average collection efficiency values were 96.6% for PM> 2.5, 85.5% for PM1.0-2.5, and 66.9% for PM< 1.0.					
36053424	6	36	theme	collection	1366:1375	arg1	%					1404:1404	96.6%	1400:1404	96.6% for PM> 2.5	1400:1416	The average collection efficiency values were 96.6% for PM> 2.5, 85.5% for PM1.0-2.5, and 66.9% for PM< 1.0.					
36053424	5	37	theme	pilot-scale	1115:1125	arg1	simulation					1127:1136	The pilot-scale simulation	1111:1136	The pilot-scale simulation	1111:1136	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	4	38	theme	industrial	933:942	arg1	one					944:946	the industrial one	929:946	the industrial one with the use of a Venturi scrubber in fine particle collection	929:1009	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	4	39	from	innovation	895:904	arg1	situation					911:919	a situation	909:919	a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection	909:1009	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	8	40	theme	control	1714:1720	arg1	technologies					1722:1733	emission control technologies	1705:1733	emission control technologies	1705:1733	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	5	41	theme	concern	1327:1333	arg1	situation					1314:1322	the current situation	1302:1322	the current situation of concern about air quality	1302:1351	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	1	42	contain	have	269:272	arg2	potential					282:290	a great potential	274:290	a great potential	274:290	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	1	42	contain	have	269:272	arg1	countries					254:262	countries	254:262	countries	254:262	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	1	42	contain	have	269:272	arg1	Brazil					316:321	Brazil	316:321	Brazil	316:321	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	1	43	contain	has	146:148	arg1	demand					139:144	Energy demand	132:144	Energy demand	132:144	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	1	43	contain	has	146:148	arg2	worldwide					160:168	increased worldwide	150:168	increased worldwide	150:168	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	5	44	theme	air	1341:1343	arg1	quality					1345:1351	air quality	1341:1351	air quality	1341:1351	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	4	45	dep	using	1027:1031	arg1	addition					1015:1022	addition	1015:1022	addition	1015:1022	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	3	46	dep	necessary	521:529	arg1	emitted					670:676	emitted	670:676	emitted	670:676	Controlling these emissions is necessary; therefore, the aim was to evaluate PM collection using a rectangular Venturi scrubber (RVS), and its effects on the composition of the PM emitted.					
36053424	3	46	dep	necessary	521:529	arg1	was					551:553	was	551:553	was to evaluate PM collection using a rectangular Venturi scrubber (RVS)	551:622	Controlling these emissions is necessary; therefore, the aim was to evaluate PM collection using a rectangular Venturi scrubber (RVS), and its effects on the composition of the PM emitted.					
36053424	7	47	theme	PM<	1486:1488	arg1	filters					1494:1500	PM< 1.0 filters	1486:1500	PM< 1.0 filters	1486:1500	The ionic analysis for PM< 1.0 filters showed potassium, chloride, nitrate, and nitrite at concentrations ranging from 20.12 to 36.5 μg/m3.					
36053424	4	48	theme	scrubber	974:981	arg1	use					957:959	the use	953:959	the use of a Venturi scrubber in fine particle collection	953:1009	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	3	49	from	effects	633:639	arg1	composition					648:658	the composition	644:658	the composition of the PM	644:668	Controlling these emissions is necessary; therefore, the aim was to evaluate PM collection using a rectangular Venturi scrubber (RVS), and its effects on the composition of the PM emitted.					
36053424	8	50	theme	portable	1768:1775	arg1	samplers					1777:1784	cost-effective and efficient portable samplers	1739:1784	cost-effective and efficient portable samplers	1739:1784	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	0	51	from	collection	73:82	arg1	efficiency					18:27	the efficiency	14:27	the efficiency of a Venturi scrubber in particulate matter collection	14:82	Evaluation of the efficiency of a Venturi scrubber in particulate matter collection smaller than 2.5 µm emitted by biomass burning.					
36053424	4	52	theme	fine	986:989	arg1	collection					1000:1009	fine particle collection	986:1009	fine particle collection	986:1009	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	4	53	theme	biomass	830:836	arg1	burning					838:844	biomass burning	830:844	biomass burning	830:844	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	4	54	theme	representative	1046:1059	arg1	equipment					1081:1089	portable and representative isokinetic sampling equipment	1033:1089	portable and representative isokinetic sampling equipment of these particles	1033:1108	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	0	55	theme	biomass	115:121	arg1	burning					123:129	biomass burning	115:129	biomass burning	115:129	Evaluation of the efficiency of a Venturi scrubber in particulate matter collection smaller than 2.5 µm emitted by biomass burning.					
36053424	4	56	theme	sampling	1072:1079	arg1	equipment					1081:1089	portable and representative isokinetic sampling equipment	1033:1089	portable and representative isokinetic sampling equipment of these particles	1033:1108	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	5	57	theme	process	1161:1167	arg1	simulation					1127:1136	The pilot-scale simulation	1111:1136	The pilot-scale simulation	1111:1136	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	8	58	theme	particulate	1808:1818	arg1	materials					1820:1828	particulate materials	1808:1828	particulate materials	1808:1828	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	4	59	from	use	957:959	arg1	collection					1000:1009	fine particle collection	986:1009	fine particle collection	986:1009	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	0	60	theme	particulate	54:64	arg1	matter					66:71	particulate matter	54:71	particulate matter collection	54:82	Evaluation of the efficiency of a Venturi scrubber in particulate matter collection smaller than 2.5 µm emitted by biomass burning.					
36053424	8	61	theme	ethanol	1610:1616	arg1	plants					1628:1633	the ethanol and sugar plants	1606:1633	plants	1628:1633	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	1	62	theme	Energy	132:137	arg1	demand					139:144	Energy demand	132:144	Energy demand	132:144	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	0	63	from	efficiency	18:27	arg1	collection					73:82	particulate matter collection	54:82	particulate matter collection	54:82	Evaluation of the efficiency of a Venturi scrubber in particulate matter collection smaller than 2.5 µm emitted by biomass burning.					
36053424	4	64	theme	portable	1033:1040	arg1	equipment					1081:1089	portable and representative isokinetic sampling equipment	1033:1089	portable and representative isokinetic sampling equipment of these particles	1033:1108	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	8	65	theme	current	1842:1848	arg1	projects					1873:1880	current gas cleaning equipment projects	1842:1880	current gas cleaning equipment projects	1842:1880	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	8	66	theme	sugar	1622:1626	arg1	plants					1628:1633	the ethanol and sugar plants	1606:1633	plants	1628:1633	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	2	67	theme	chemical	428:435	arg1	composition					437:447	a complex chemical composition	418:447	a complex chemical composition	418:447	However, these energy sources generate pollutants, consisting of particulate matter (PM) with a complex chemical composition, such as sugarcane bagasse (SB) burning.					
36053424	5	68	theme	biomass	1145:1151	arg1	process					1161:1167	the biomass burning process	1141:1167	the biomass burning process	1141:1167	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	8	69	theme	cleaning	1854:1861	arg1	projects					1873:1880	current gas cleaning equipment projects	1842:1880	current gas cleaning equipment projects	1842:1880	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	2	70	theme	bagasse	468:474	arg1	burning					481:487	sugarcane bagasse (SB) burning	458:487	sugarcane bagasse (SB) burning	458:487	However, these energy sources generate pollutants, consisting of particulate matter (PM) with a complex chemical composition, such as sugarcane bagasse (SB) burning.					
36053424	0	71	from	scrubber	42:49	arg1	collection					73:82	particulate matter collection	54:82	particulate matter collection	54:82	Evaluation of the efficiency of a Venturi scrubber in particulate matter collection smaller than 2.5 µm emitted by biomass burning.					
36053424	8	72	dep	continue	1640:1647	arg1	will					1635:1638	will	1635:1638	will	1635:1638	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	5	73	theme	parameters	1230:1239	arg1	sampling					1189:1196	the representative sampling	1170:1196	the representative sampling of fine particles and obtaining parameters	1170:1239	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	4	74	theme	particles	1100:1108	arg1	equipment					1081:1089	portable and representative isokinetic sampling equipment	1033:1089	portable and representative isokinetic sampling equipment of these particles	1033:1108	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	0	75	theme	efficiency	18:27	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the efficiency of a Venturi scrubber in particulate matter collection	0:82	Evaluation of the efficiency of a Venturi scrubber in particulate matter collection smaller than 2.5 µm emitted by biomass burning.					
36053424	8	76	theme	sugarcane	1678:1686	arg1	burning					1696:1702	sugarcane bagasse burning	1678:1702	sugarcane bagasse burning	1678:1702	As the ethanol and sugar plants will continue to generate electricity with sugarcane bagasse burning, emission control technologies and cost-effective and efficient portable samplers are needed to monitor particulate materials and improve current gas cleaning equipment projects.					
36053424	2	77	theme	particulate	389:399	arg1	PM					409:410	PM	409:410	PM	409:410	However, these energy sources generate pollutants, consisting of particulate matter (PM) with a complex chemical composition, such as sugarcane bagasse (SB) burning.					
36053424	2	77	theme	particulate	389:399	arg1	matter					401:406	particulate matter	389:406	particulate matter (PM) with a complex chemical composition	389:447	However, these energy sources generate pollutants, consisting of particulate matter (PM) with a complex chemical composition, such as sugarcane bagasse (SB) burning.					
36053424	5	78	theme	fine	1201:1204	arg1	particles					1206:1214	fine particles	1201:1214	fine particles	1201:1214	The pilot-scale simulation of the biomass burning process, the representative sampling of fine particles and obtaining parameters to control pollutant emissions for a Venturi scrubber, meets the current situation of concern about air quality.					
36053424	4	79	theme	biomass	714:720	arg1	use					707:709	the appropriate use	691:709	the appropriate use of biomass	691:720	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	4	79	theme	biomass	714:720	arg1	fuel					739:742	an industrial fuel	725:742	an industrial fuel	725:742	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	4	79	theme	biomass	714:720	arg1	need					761:764	the emerging need	748:764	the emerging need for a technique capable of efficiently removing pollutants from biomass burning	748:844	Considering the appropriate use of biomass as an industrial fuel and the emerging need for a technique capable of efficiently removing pollutants from biomass burning, this study shows the control of emissions as an innovation in a situation such as the industrial one with the use of a Venturi scrubber in fine particle collection, in addition to using portable and representative isokinetic sampling equipment of these particles.					
36053424	0	80	theme	scrubber	42:49	arg1	efficiency					18:27	the efficiency	14:27	the efficiency of a Venturi scrubber in particulate matter collection	14:82	Evaluation of the efficiency of a Venturi scrubber in particulate matter collection smaller than 2.5 µm emitted by biomass burning.					
36053424	1	81	theme	great	276:280	arg1	potential					282:290	a great potential	274:290	a great potential	274:290	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	2	82	theme	SB	477:478	arg1	burning					481:487	sugarcane bagasse (SB) burning	458:487	sugarcane bagasse (SB) burning	458:487	However, these energy sources generate pollutants, consisting of particulate matter (PM) with a complex chemical composition, such as sugarcane bagasse (SB) burning.					
36053424	3	83	theme	Venturi	601:607	arg1	RVS					619:621	RVS	619:621	RVS	619:621	Controlling these emissions is necessary; therefore, the aim was to evaluate PM collection using a rectangular Venturi scrubber (RVS), and its effects on the composition of the PM emitted.					
36053424	3	83	theme	Venturi	601:607	arg1	scrubber					609:616	a rectangular Venturi scrubber	587:616	a rectangular Venturi scrubber (RVS)	587:622	Controlling these emissions is necessary; therefore, the aim was to evaluate PM collection using a rectangular Venturi scrubber (RVS), and its effects on the composition of the PM emitted.					
36053424	2	84	with	matter	401:406	arg1	composition					437:447	a complex chemical composition	418:447	a complex chemical composition	418:447	However, these energy sources generate pollutants, consisting of particulate matter (PM) with a complex chemical composition, such as sugarcane bagasse (SB) burning.					
36053424	6	85	theme	efficiency	1377:1386	arg1	values					1388:1393	The average collection efficiency values	1354:1393	The average collection efficiency values	1354:1393	The average collection efficiency values were 96.6% for PM> 2.5, 85.5% for PM1.0-2.5, and 66.9% for PM< 1.0.					
36053424	6	85	theme	efficiency	1377:1386	arg1	%					1404:1404	96.6%	1400:1404	96.6% for PM> 2.5	1400:1416	The average collection efficiency values were 96.6% for PM> 2.5, 85.5% for PM1.0-2.5, and 66.9% for PM< 1.0.					
36053424	1	86	theme	solutions	205:213	arg1	one					194:196	one	194:196	one	194:196	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	1	86	theme	solutions	205:213	arg1	solutions					205:213	the solutions	201:213	the solutions most used by industries	201:237	Energy demand has increased worldwide, and biomass burning is one of the solutions most used by industries, especially in countries that have a great potential in agriculture, such as Brazil.					
36053424	6	87	theme	average	1358:1364	arg1	values					1388:1393	The average collection efficiency values	1354:1393	The average collection efficiency values	1354:1393	The average collection efficiency values were 96.6% for PM> 2.5, 85.5% for PM1.0-2.5, and 66.9% for PM< 1.0.					
36053424	6	87	theme	average	1358:1364	arg1	%					1404:1404	96.6%	1400:1404	96.6% for PM> 2.5	1400:1416	The average collection efficiency values were 96.6% for PM> 2.5, 85.5% for PM1.0-2.5, and 66.9% for PM< 1.0.					
36872509	12	0	theme	PT	1725:1726	arg1	males					1728:1732	PT males	1725:1732	PT males	1725:1732	At day 2, the microbial composition in PT/CS females was dominated by Bifidobacterium; whereas PT males demonstrated elevated levels of Roseburia (p < 0.01).					
36872509	13	1	theme	=	1870:1870	arg1	0.0002					1872:1877	p = 0.0002	1868:1877	p = 0.0002	1868:1877	PT/CS males had significantly elevated ileum injury scores compared to females (p = 0.0002).					
36872509	13	1	theme	=	1870:1870	arg1	females					1859:1865	females	1859:1865	females (p = 0.0002)	1859:1878	PT/CS males had significantly elevated ileum injury scores compared to females (p = 0.0002).					
36872509	2	2	theme	"	293:293	arg1	phenotype					295:303	the "pathobiome" phenotype	278:303	the "pathobiome" phenotype induced by multicompartmental injuries and chronic stress	278:361	We hypothesized that the "pathobiome" phenotype induced by multicompartmental injuries and chronic stress is host sex specific with unique microbiome signatures.					
36872509	2	2	theme	"	293:293	arg1	specific					375:382	specific	375:382	specific	375:382	We hypothesized that the "pathobiome" phenotype induced by multicompartmental injuries and chronic stress is host sex specific with unique microbiome signatures.					
36872509	10	3	dep	RESULTS	1350:1356	arg1	had					1379:1381	had	1379:1381	had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS	1379:1537	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	3	4	dep	injury	547:552	arg1	pseudofractures					616:630	bifemoral pseudofractures	606:630	bifemoral pseudofractures	606:630	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	4	dep	injury	547:552	arg1	cecectomy					595:603	cecectomy	595:603	cecectomy	595:603	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	4	dep	injury	547:552	arg1	contusion					565:573	lung contusion	560:573	lung contusion	560:573	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	4	dep	injury	547:552	arg1	shock					588:592	hemorrhagic shock	576:592	hemorrhagic shock	576:592	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	4	dep	injury	547:552	arg1	stress					674:679	daily chronic restraint stress	650:679	daily chronic restraint stress	650:679	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	2	5	theme	microbiome	396:405	arg1	signatures					407:416	unique microbiome signatures	389:416	unique microbiome signatures	389:416	We hypothesized that the "pathobiome" phenotype induced by multicompartmental injuries and chronic stress is host sex specific with unique microbiome signatures.					
36872509	3	6	theme	naive	692:696	arg1	controls					698:705	naive controls	692:705	naive controls	692:705	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	7	7	theme	binding	1118:1124	arg1	LBP					1135:1137	LBP	1135:1137	LBP	1135:1137	Intestinal permeability was evaluated by plasma occludin and lipopolysaccharide binding protein (LBP).					
36872509	7	7	theme	binding	1118:1124	arg1	protein					1126:1132	lipopolysaccharide binding protein	1099:1132	lipopolysaccharide binding protein (LBP)	1099:1138	Intestinal permeability was evaluated by plasma occludin and lipopolysaccharide binding protein (LBP).					
36872509	2	8	theme	multicompartmental	316:333	arg1	injuries					335:342	multicompartmental injuries	316:342	multicompartmental injuries	316:342	We hypothesized that the "pathobiome" phenotype induced by multicompartmental injuries and chronic stress is host sex specific with unique microbiome signatures.					
36872509	15	9	theme	CONCLUSIONS	2004:2014	arg1	trauma					2035:2040	CONCLUSIONS Multicompartmental trauma	2004:2040	CONCLUSIONS Multicompartmental trauma	2004:2040	CONCLUSIONS Multicompartmental trauma induces significant alterations in microbiome diversity and taxa, but these signatures differ by host sex.					
36872509	12	10	theme	PT/CS	1669:1673	arg1	females					1675:1681	PT/CS females	1669:1681	PT/CS females	1669:1681	At day 2, the microbial composition in PT/CS females was dominated by Bifidobacterium; whereas PT males demonstrated elevated levels of Roseburia (p < 0.01).					
36872509	7	11	theme	plasma	1079:1084	arg1	occludin					1086:1093	plasma occludin	1079:1093	plasma occludin	1079:1093	Intestinal permeability was evaluated by plasma occludin and lipopolysaccharide binding protein (LBP).					
36872509	3	12	theme	lung	560:563	arg1	pseudofractures					616:630	bifemoral pseudofractures	606:630	bifemoral pseudofractures	606:630	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	12	theme	lung	560:563	arg1	cecectomy					595:603	cecectomy	595:603	cecectomy	595:603	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	12	theme	lung	560:563	arg1	contusion					565:573	lung contusion	560:573	lung contusion	560:573	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	12	theme	lung	560:563	arg1	shock					588:592	hemorrhagic shock	576:592	hemorrhagic shock	576:592	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	2	13	theme	unique	389:394	arg1	signatures					407:416	unique microbiome signatures	389:416	unique microbiome signatures	389:416	We hypothesized that the "pathobiome" phenotype induced by multicompartmental injuries and chronic stress is host sex specific with unique microbiome signatures.					
36872509	5	14	theme	alpha	842:846	arg1	diversity					848:856	Microbial alpha diversity	832:856	Microbial alpha diversity	832:856	Microbial alpha diversity was assessed using Chao1 (number of different unique species) and Shannon (species richness and evenness) indices.					
36872509	1	15	from	impact	213:218	arg1	dysbiosis					230:238	dysbiosis	230:238	dysbiosis	230:238	BACKGROUND Previous preclinical studies have demonstrated an altered gut microbiome after traumatic injury; however, the impact of sex on dysbiosis remains unknown.					
36872509	5	16	theme	unique	904:909	arg1	species					911:917	different unique species	894:917	different unique species	894:917	Microbial alpha diversity was assessed using Chao1 (number of different unique species) and Shannon (species richness and evenness) indices.					
36872509	4	17	theme	Fecal	708:712	arg1	microbiome					714:723	Fecal microbiome	708:723	Fecal microbiome	708:723	Fecal microbiome was measured on days 0 and 2 using high-throughput 16S rRNA sequencing and QIIME2 bioinformatics analyses.					
36872509	13	18	theme	injury	1833:1838	arg1	scores					1840:1845	significantly elevated ileum injury scores	1804:1845	significantly elevated ileum injury scores	1804:1845	PT/CS males had significantly elevated ileum injury scores compared to females (p = 0.0002).					
36872509	1	19	theme	gut	161:163	arg1	microbiome					165:174	an altered gut microbiome	150:174	an altered gut microbiome	150:174	BACKGROUND Previous preclinical studies have demonstrated an altered gut microbiome after traumatic injury; however, the impact of sex on dysbiosis remains unknown.					
36872509	12	20	theme	microbial	1644:1652	arg1	composition					1654:1664	the microbial composition	1640:1664	the microbial composition in PT/CS females	1640:1681	At day 2, the microbial composition in PT/CS females was dominated by Bifidobacterium; whereas PT males demonstrated elevated levels of Roseburia (p < 0.01).					
36872509	6	21	theme	principle	1007:1015	arg1	analysis					1028:1035	principle coordinate analysis	1007:1035	principle coordinate analysis	1007:1035	Beta-diversity was assessed using principle coordinate analysis.					
36872509	0	22	theme	Gut	76:78	arg1	Microbiome					80:89	the Gut Microbiome	72:89	the Gut Microbiome	72:89	Multicompartmental Traumatic Injury Induces Sex-Specific Alterations in the Gut Microbiome.					
36872509	3	23	theme	bifemoral	606:614	arg1	contusion					565:573	lung contusion	560:573	lung contusion	560:573	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	23	theme	bifemoral	606:614	arg1	pseudofractures					616:630	bifemoral pseudofractures	606:630	bifemoral pseudofractures	606:630	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	15	24	theme	significant	2050:2060	arg1	alterations					2062:2072	significant alterations	2050:2072	significant alterations in microbiome diversity	2050:2096	CONCLUSIONS Multicompartmental trauma induces significant alterations in microbiome diversity and taxa, but these signatures differ by host sex.					
36872509	10	25	theme	Shannon	1430:1436	arg1	indices					1438:1444	Shannon indices	1430:1444	Shannon indices	1430:1444	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	10	25	theme	Shannon	1430:1436	arg1	Chao1					1423:1427	Chao1	1423:1427	Chao1	1423:1427	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	13	26	theme	elevated	1818:1825	arg1	scores					1840:1845	significantly elevated ileum injury scores	1804:1845	significantly elevated ileum injury scores	1804:1845	PT/CS males had significantly elevated ileum injury scores compared to females (p = 0.0002).					
36872509	3	27	dep	METHODS	419:425	arg1	rats					468:471	proestrus female Sprague-Dawley rats	436:471	proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks	436:501	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	27	dep	METHODS	419:425	arg1	Male					427:430	Male	427:430	Male	427:430	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	27	dep	METHODS	419:425	arg1	8/group					478:484	n = 8/group	474:484	n = 8/group	474:484	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	1	28	theme	BACKGROUND	92:101	arg1	studies					124:130	BACKGROUND Previous preclinical studies	92:130	BACKGROUND Previous preclinical studies	92:130	BACKGROUND Previous preclinical studies have demonstrated an altered gut microbiome after traumatic injury; however, the impact of sex on dysbiosis remains unknown.					
36872509	13	29	theme	PT/CS	1788:1792	arg1	males					1794:1798	PT/CS males	1788:1798	PT/CS males	1788:1798	PT/CS males had significantly elevated ileum injury scores compared to females (p = 0.0002).					
36872509	3	30	theme	female	446:451	arg1	rats					468:471	proestrus female Sprague-Dawley rats	436:471	proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks	436:501	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	30	theme	female	446:451	arg1	8/group					478:484	n = 8/group	474:484	n = 8/group	474:484	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	0	31	theme	Multicompartmental	0:17	arg1	Injury					29:34	Multicompartmental Traumatic Injury	0:34	Multicompartmental Traumatic Injury	0:34	Multicompartmental Traumatic Injury Induces Sex-Specific Alterations in the Gut Microbiome.					
36872509	15	32	theme	host	2139:2142	arg1	sex					2144:2146	host sex	2139:2146	host sex	2139:2146	CONCLUSIONS Multicompartmental trauma induces significant alterations in microbiome diversity and taxa, but these signatures differ by host sex.					
36872509	1	33	theme	preclinical	112:122	arg1	studies					124:130	BACKGROUND Previous preclinical studies	92:130	BACKGROUND Previous preclinical studies	92:130	BACKGROUND Previous preclinical studies have demonstrated an altered gut microbiome after traumatic injury; however, the impact of sex on dysbiosis remains unknown.					
36872509	15	34	from	taxa	2102:2105	arg1	diversity					2088:2096	microbiome diversity	2077:2096	microbiome diversity	2077:2096	CONCLUSIONS Multicompartmental trauma induces significant alterations in microbiome diversity and taxa, but these signatures differ by host sex.					
36872509	5	35	dep	Chao1	877:881	arg1	number					884:889	number	884:889	number of different unique species	884:917	Microbial alpha diversity was assessed using Chao1 (number of different unique species) and Shannon (species richness and evenness) indices.					
36872509	5	35	dep	Chao1	877:881	arg1	indices					964:970	indices	964:970	indices	964:970	Microbial alpha diversity was assessed using Chao1 (number of different unique species) and Shannon (species richness and evenness) indices.					
36872509	14	36	dep	higher	1901:1906	arg1	0.004					1945:1949	p = 0.004	1941:1949	p = 0.004	1941:1949	Plasma occludin was higher in PT males compared to females (p = 0.004); plasma LBP was elevated in PT/CS males (p = 0.03).					
36872509	14	37	theme	Plasma	1881:1886	arg1	occludin					1888:1895	Plasma occludin	1881:1895	Plasma occludin	1881:1895	Plasma occludin was higher in PT males compared to females (p = 0.004); plasma LBP was elevated in PT/CS males (p = 0.03).					
36872509	0	38	theme	Sex-Specific	44:55	arg1	Alterations					57:67	Sex-Specific Alterations	44:67	Sex-Specific Alterations	44:67	Multicompartmental Traumatic Injury Induces Sex-Specific Alterations in the Gut Microbiome.					
36872509	10	39	from	injury	1516:1521	arg1	PT					1526:1527	PT	1526:1527	PT	1526:1527	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	10	39	from	injury	1516:1521	arg1	PT/CS					1533:1537	PT/CS	1533:1537	PT/CS	1533:1537	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	12	40	theme	elevated	1747:1754	arg1	<					1779:1779	p < 0.01	1777:1784	p < 0.01	1777:1784	At day 2, the microbial composition in PT/CS females was dominated by Bifidobacterium; whereas PT males demonstrated elevated levels of Roseburia (p < 0.01).					
36872509	12	40	theme	elevated	1747:1754	arg1	levels					1756:1761	elevated levels	1747:1761	elevated levels of Roseburia (p < 0.01)	1747:1785	At day 2, the microbial composition in PT/CS females was dominated by Bifidobacterium; whereas PT males demonstrated elevated levels of Roseburia (p < 0.01).					
36872509	12	41	from	composition	1654:1664	arg1	females					1675:1681	PT/CS females	1669:1681	PT/CS females	1669:1681	At day 2, the microbial composition in PT/CS females was dominated by Bifidobacterium; whereas PT males demonstrated elevated levels of Roseburia (p < 0.01).					
36872509	14	42	theme	p	1941:1941	arg1	0.004					1945:1949	p = 0.004	1941:1949	p = 0.004	1941:1949	Plasma occludin was higher in PT males compared to females (p = 0.004); plasma LBP was elevated in PT/CS males (p = 0.03).					
36872509	4	43	theme	bioinformatics	807:820	arg1	analyses					822:829	QIIME2 bioinformatics analyses	800:829	QIIME2 bioinformatics analyses	800:829	Fecal microbiome was measured on days 0 and 2 using high-throughput 16S rRNA sequencing and QIIME2 bioinformatics analyses.					
36872509	16	44	theme	severe	2251:2256	arg1	trauma					2258:2263	severe trauma	2251:2263	severe trauma	2251:2263	These findings suggest that sex is an important biological variable that may influence outcomes after severe trauma and critical illness.					
36872509	14	45	theme	=	1943:1943	arg1	0.004					1945:1949	p = 0.004	1941:1949	p = 0.004	1941:1949	Plasma occludin was higher in PT males compared to females (p = 0.004); plasma LBP was elevated in PT/CS males (p = 0.03).					
36872509	12	46	theme	Roseburia	1766:1774	arg1	<					1779:1779	p < 0.01	1777:1784	p < 0.01	1777:1784	At day 2, the microbial composition in PT/CS females was dominated by Bifidobacterium; whereas PT males demonstrated elevated levels of Roseburia (p < 0.01).					
36872509	12	46	theme	Roseburia	1766:1774	arg1	levels					1756:1761	elevated levels	1747:1761	elevated levels of Roseburia (p < 0.01)	1747:1785	At day 2, the microbial composition in PT/CS females was dominated by Bifidobacterium; whereas PT males demonstrated elevated levels of Roseburia (p < 0.01).					
36872509	4	47	theme	high-throughput	760:774	arg1	sequencing					785:794	high-throughput 16S rRNA sequencing	760:794	high-throughput 16S rRNA sequencing	760:794	Fecal microbiome was measured on days 0 and 2 using high-throughput 16S rRNA sequencing and QIIME2 bioinformatics analyses.					
36872509	11	48	theme	Beta	1540:1543	arg1	diversity					1545:1553	Beta diversity	1540:1553	Beta diversity	1540:1553	Beta diversity also differed significantly between males and females after PT (p = 0.01).					
36872509	3	49	theme	aged	487:490	arg1	rats					468:471	proestrus female Sprague-Dawley rats	436:471	proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks	436:501	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	49	theme	aged	487:490	arg1	8/group					478:484	n = 8/group	474:484	n = 8/group	474:484	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	12	50	theme	p	1777:1777	arg1	<					1779:1779	p < 0.01	1777:1784	p < 0.01	1777:1784	At day 2, the microbial composition in PT/CS females was dominated by Bifidobacterium; whereas PT males demonstrated elevated levels of Roseburia (p < 0.01).					
36872509	12	50	theme	p	1777:1777	arg1	levels					1756:1761	elevated levels	1747:1761	elevated levels of Roseburia (p < 0.01)	1747:1785	At day 2, the microbial composition in PT/CS females was dominated by Bifidobacterium; whereas PT males demonstrated elevated levels of Roseburia (p < 0.01).					
36872509	3	51	theme	chronic	656:662	arg1	stress					674:679	daily chronic restraint stress	650:679	daily chronic restraint stress	650:679	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	4	52	theme	rRNA	780:783	arg1	sequencing					785:794	high-throughput 16S rRNA sequencing	760:794	high-throughput 16S rRNA sequencing	760:794	Fecal microbiome was measured on days 0 and 2 using high-throughput 16S rRNA sequencing and QIIME2 bioinformatics analyses.					
36872509	10	53	contain	had	1379:1381	arg2	alpha-diversity					1406:1420	significantly elevated alpha-diversity	1383:1420	significantly elevated alpha-diversity (Chao1, Shannon indices)	1383:1445	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	10	53	contain	had	1379:1381	arg1	females					1371:1377	females	1371:1377	females	1371:1377	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	10	54	theme	2-days	1504:1509	arg1	injury					1516:1521	2-days post injury	1504:1521	2-days post injury in PT and PT/CS	1504:1537	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	16	55	theme	important	2187:2195	arg1	variable					2208:2215	an important biological variable	2184:2215	an important biological variable that may influence outcomes after severe trauma and critical illness	2184:2284	These findings suggest that sex is an important biological variable that may influence outcomes after severe trauma and critical illness.					
36872509	16	55	theme	important	2187:2195	arg1	sex					2177:2179	sex	2177:2179	sex	2177:2179	These findings suggest that sex is an important biological variable that may influence outcomes after severe trauma and critical illness.					
36872509	8	56	theme	ileum	1166:1170	arg1	evaluation					1152:1161	Histologic evaluation	1141:1161	Histologic evaluation of ileum and colon tissues	1141:1188	Histologic evaluation of ileum and colon tissues were scored for injury by a blinded pathologist.					
36872509	17	57	theme	EVIDENCE	2296:2303	arg1	LEVEL					2287:2291	LEVEL	2287:2291	LEVEL OF EVIDENCE	2287:2303	LEVEL OF EVIDENCE Not applicable - basic science.					
36872509	7	58	theme	lipopolysaccharide	1099:1116	arg1	LBP					1135:1137	LBP	1135:1137	LBP	1135:1137	Intestinal permeability was evaluated by plasma occludin and lipopolysaccharide binding protein (LBP).					
36872509	7	58	theme	lipopolysaccharide	1099:1116	arg1	protein					1126:1132	lipopolysaccharide binding protein	1099:1132	lipopolysaccharide binding protein (LBP)	1099:1138	Intestinal permeability was evaluated by plasma occludin and lipopolysaccharide binding protein (LBP).					
36872509	2	59	theme	pathobiome	283:292	arg1	phenotype					295:303	the "pathobiome" phenotype	278:303	the "pathobiome" phenotype induced by multicompartmental injuries and chronic stress	278:361	We hypothesized that the "pathobiome" phenotype induced by multicompartmental injuries and chronic stress is host sex specific with unique microbiome signatures.					
36872509	2	59	theme	pathobiome	283:292	arg1	specific					375:382	specific	375:382	specific	375:382	We hypothesized that the "pathobiome" phenotype induced by multicompartmental injuries and chronic stress is host sex specific with unique microbiome signatures.					
36872509	15	60	theme	microbiome	2077:2086	arg1	diversity					2088:2096	microbiome diversity	2077:2096	microbiome diversity	2077:2096	CONCLUSIONS Multicompartmental trauma induces significant alterations in microbiome diversity and taxa, but these signatures differ by host sex.					
36872509	15	61	theme	Multicompartmental	2016:2033	arg1	trauma					2035:2040	CONCLUSIONS Multicompartmental trauma	2004:2040	CONCLUSIONS Multicompartmental trauma	2004:2040	CONCLUSIONS Multicompartmental trauma induces significant alterations in microbiome diversity and taxa, but these signatures differ by host sex.					
36872509	8	62	theme	colon	1176:1180	arg1	evaluation					1152:1161	Histologic evaluation	1141:1161	Histologic evaluation of ileum and colon tissues	1141:1188	Histologic evaluation of ileum and colon tissues were scored for injury by a blinded pathologist.					
36872509	10	63	theme	elevated	1397:1404	arg1	alpha-diversity					1406:1420	significantly elevated alpha-diversity	1383:1420	significantly elevated alpha-diversity (Chao1, Shannon indices)	1383:1445	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	5	64	theme	Microbial	832:840	arg1	diversity					848:856	Microbial alpha diversity	832:856	Microbial alpha diversity	832:856	Microbial alpha diversity was assessed using Chao1 (number of different unique species) and Shannon (species richness and evenness) indices.					
36872509	18	65	dep	TYPE	2343:2346	arg1	Basic					2348:2352	Basic	2348:2352	Basic	2348:2352	STUDY TYPE Basic Science.					
36872509	14	66	theme	=	1995:1995	arg1	p					1993:1993	p = 0.03	1993:2000	p = 0.03	1993:2000	Plasma occludin was higher in PT males compared to females (p = 0.004); plasma LBP was elevated in PT/CS males (p = 0.03).					
36872509	14	66	theme	=	1995:1995	arg1	males					1986:1990	PT/CS males	1980:1990	PT/CS males (p = 0.03)	1980:2001	Plasma occludin was higher in PT males compared to females (p = 0.004); plasma LBP was elevated in PT/CS males (p = 0.03).					
36872509	7	67	theme	Intestinal	1038:1047	arg1	permeability					1049:1060	Intestinal permeability	1038:1060	Intestinal permeability	1038:1060	Intestinal permeability was evaluated by plasma occludin and lipopolysaccharide binding protein (LBP).					
36872509	3	68	theme	hemorrhagic	576:586	arg1	contusion					565:573	lung contusion	560:573	lung contusion	560:573	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	68	theme	hemorrhagic	576:586	arg1	shock					588:592	hemorrhagic shock	576:592	hemorrhagic shock	576:592	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	5	69	theme	different	894:902	arg1	species					911:917	different unique species	894:917	different unique species	894:917	Microbial alpha diversity was assessed using Chao1 (number of different unique species) and Shannon (species richness and evenness) indices.					
36872509	8	70	theme	blinded	1218:1224	arg1	pathologist					1226:1236	a blinded pathologist	1216:1236	a blinded pathologist	1216:1236	Histologic evaluation of ileum and colon tissues were scored for injury by a blinded pathologist.					
36872509	14	71	theme	PT/CS	1980:1984	arg1	p					1993:1993	p = 0.03	1993:2000	p = 0.03	1993:2000	Plasma occludin was higher in PT males compared to females (p = 0.004); plasma LBP was elevated in PT/CS males (p = 0.03).					
36872509	14	71	theme	PT/CS	1980:1984	arg1	males					1986:1990	PT/CS males	1980:1990	PT/CS males (p = 0.03)	1980:2001	Plasma occludin was higher in PT males compared to females (p = 0.004); plasma LBP was elevated in PT/CS males (p = 0.03).					
36872509	13	72	theme	ileum	1827:1831	arg1	scores					1840:1845	significantly elevated ileum injury scores	1804:1845	significantly elevated ileum injury scores	1804:1845	PT/CS males had significantly elevated ileum injury scores compared to females (p = 0.0002).					
36872509	13	73	theme	p	1868:1868	arg1	0.0002					1872:1877	p = 0.0002	1868:1877	p = 0.0002	1868:1877	PT/CS males had significantly elevated ileum injury scores compared to females (p = 0.0002).					
36872509	13	73	theme	p	1868:1868	arg1	females					1859:1865	females	1859:1865	females (p = 0.0002)	1859:1878	PT/CS males had significantly elevated ileum injury scores compared to females (p = 0.0002).					
36872509	1	74	theme	altered	153:159	arg1	microbiome					165:174	an altered gut microbiome	150:174	an altered gut microbiome	150:174	BACKGROUND Previous preclinical studies have demonstrated an altered gut microbiome after traumatic injury; however, the impact of sex on dysbiosis remains unknown.					
36872509	6	75	theme	coordinate	1017:1026	arg1	analysis					1028:1035	principle coordinate analysis	1007:1035	principle coordinate analysis	1007:1035	Beta-diversity was assessed using principle coordinate analysis.					
36872509	2	76	theme	chronic	348:354	arg1	stress					356:361	chronic stress	348:361	chronic stress	348:361	We hypothesized that the "pathobiome" phenotype induced by multicompartmental injuries and chronic stress is host sex specific with unique microbiome signatures.					
36872509	5	77	dep	species	933:939	arg1	species					933:939	species richness and evenness	933:961	species richness and evenness	933:961	Microbial alpha diversity was assessed using Chao1 (number of different unique species) and Shannon (species richness and evenness) indices.					
36872509	5	77	dep	species	933:939	arg1	evenness					954:961	evenness	954:961	evenness	954:961	Microbial alpha diversity was assessed using Chao1 (number of different unique species) and Shannon (species richness and evenness) indices.					
36872509	5	77	dep	species	933:939	arg1	richness					941:948	richness	941:948	richness	941:948	Microbial alpha diversity was assessed using Chao1 (number of different unique species) and Shannon (species richness and evenness) indices.					
36872509	1	78	theme	traumatic	182:190	arg1	injury					192:197	traumatic injury	182:197	traumatic injury	182:197	BACKGROUND Previous preclinical studies have demonstrated an altered gut microbiome after traumatic injury; however, the impact of sex on dysbiosis remains unknown.					
36872509	8	79	dep	ileum	1166:1170	arg1	tissues					1182:1188	tissues	1182:1188	tissues	1182:1188	Histologic evaluation of ileum and colon tissues were scored for injury by a blinded pathologist.					
36872509	15	80	from	alterations	2062:2072	arg1	diversity					2088:2096	microbiome diversity	2077:2096	microbiome diversity	2077:2096	CONCLUSIONS Multicompartmental trauma induces significant alterations in microbiome diversity and taxa, but these signatures differ by host sex.					
36872509	9	81	theme	p	1311:1311	arg1	<					1313:1313	p < 0.05	1311:1318	p < 0.05 between males versus females	1311:1347	Analyses were performed in GraphPad and R, with significance defined as p < 0.05 between males versus females.					
36872509	14	82	theme	PT	1911:1912	arg1	males					1914:1918	PT males	1911:1918	PT males	1911:1918	Plasma occludin was higher in PT males compared to females (p = 0.004); plasma LBP was elevated in PT/CS males (p = 0.03).					
36872509	5	83	theme	species	911:917	arg1	number					884:889	number	884:889	number of different unique species	884:917	Microbial alpha diversity was assessed using Chao1 (number of different unique species) and Shannon (species richness and evenness) indices.					
36872509	13	84	contain	had	1800:1802	arg1	males					1794:1798	PT/CS males	1788:1798	PT/CS males	1788:1798	PT/CS males had significantly elevated ileum injury scores compared to females (p = 0.0002).					
36872509	13	84	contain	had	1800:1802	arg2	scores					1840:1845	significantly elevated ileum injury scores	1804:1845	significantly elevated ileum injury scores	1804:1845	PT/CS males had significantly elevated ileum injury scores compared to females (p = 0.0002).					
36872509	0	85	theme	Traumatic	19:27	arg1	Injury					29:34	Multicompartmental Traumatic Injury	0:34	Multicompartmental Traumatic Injury	0:34	Multicompartmental Traumatic Injury Induces Sex-Specific Alterations in the Gut Microbiome.					
36872509	1	86	theme	Previous	103:110	arg1	studies					124:130	BACKGROUND Previous preclinical studies	92:130	BACKGROUND Previous preclinical studies	92:130	BACKGROUND Previous preclinical studies have demonstrated an altered gut microbiome after traumatic injury; however, the impact of sex on dysbiosis remains unknown.					
36872509	14	87	theme	plasma	1953:1958	arg1	LBP					1960:1962	plasma LBP	1953:1962	plasma LBP	1953:1962	Plasma occludin was higher in PT males compared to females (p = 0.004); plasma LBP was elevated in PT/CS males (p = 0.03).					
36872509	3	88	theme	Sprague-Dawley	453:466	arg1	rats					468:471	proestrus female Sprague-Dawley rats	436:471	proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks	436:501	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	88	theme	Sprague-Dawley	453:466	arg1	8/group					478:484	n = 8/group	474:484	n = 8/group	474:484	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	16	89	dep	important	2187:2195	arg1	biological					2197:2206	biological	2197:2206	biological	2197:2206	These findings suggest that sex is an important biological variable that may influence outcomes after severe trauma and critical illness.					
36872509	1	90	theme	sex	223:225	arg1	impact					213:218	the impact	209:218	the impact of sex on dysbiosis	209:238	BACKGROUND Previous preclinical studies have demonstrated an altered gut microbiome after traumatic injury; however, the impact of sex on dysbiosis remains unknown.					
36872509	3	91	theme	proestrus	436:444	arg1	rats					468:471	proestrus female Sprague-Dawley rats	436:471	proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks	436:501	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	91	theme	proestrus	436:444	arg1	8/group					478:484	n = 8/group	474:484	n = 8/group	474:484	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	10	92	theme	p	1466:1466	arg1	males					1459:1463	males	1459:1463	males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS	1459:1537	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	10	92	theme	p	1466:1466	arg1	<					1468:1468	p < 0.05	1466:1473	p < 0.05	1466:1473	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	3	93	theme	=	476:476	arg1	rats					468:471	proestrus female Sprague-Dawley rats	436:471	proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks	436:501	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	93	theme	=	476:476	arg1	8/group					478:484	n = 8/group	474:484	n = 8/group	474:484	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	94	dep	rats	468:471	arg1	weeks					497:501	9-11 weeks	492:501	proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks	436:501	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	95	theme	restraint	664:672	arg1	stress					674:679	daily chronic restraint stress	650:679	daily chronic restraint stress	650:679	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	4	96	theme	QIIME2	800:805	arg1	analyses					822:829	QIIME2 bioinformatics analyses	800:829	QIIME2 bioinformatics analyses	800:829	Fecal microbiome was measured on days 0 and 2 using high-throughput 16S rRNA sequencing and QIIME2 bioinformatics analyses.					
36872509	16	97	theme	critical	2269:2276	arg1	illness					2278:2284	critical illness	2269:2284	critical illness	2269:2284	These findings suggest that sex is an important biological variable that may influence outcomes after severe trauma and critical illness.					
36872509	10	98	dep	alpha-diversity	1406:1420	arg1	indices					1438:1444	Shannon indices	1430:1444	Shannon indices	1430:1444	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	10	98	dep	alpha-diversity	1406:1420	arg1	Chao1					1423:1427	Chao1	1423:1427	Chao1	1423:1427	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	3	99	theme	daily	650:654	arg1	stress					674:679	daily chronic restraint stress	650:679	daily chronic restraint stress	650:679	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	8	100	theme	Histologic	1141:1150	arg1	evaluation					1152:1161	Histologic evaluation	1141:1161	Histologic evaluation of ileum and colon tissues	1141:1188	Histologic evaluation of ileum and colon tissues were scored for injury by a blinded pathologist.					
36872509	2	101	with	specific	375:382	arg1	signatures					407:416	unique microbiome signatures	389:416	unique microbiome signatures	389:416	We hypothesized that the "pathobiome" phenotype induced by multicompartmental injuries and chronic stress is host sex specific with unique microbiome signatures.					
36872509	10	102	theme	post	1511:1514	arg1	injury					1516:1521	2-days post injury	1504:1521	2-days post injury in PT and PT/CS	1504:1537	RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared to males (p < 0.05) which was no longer present 2-days post injury in PT and PT/CS.					
36872509	4	103	theme	16S	776:778	arg1	sequencing					785:794	high-throughput 16S rRNA sequencing	760:794	high-throughput 16S rRNA sequencing	760:794	Fecal microbiome was measured on days 0 and 2 using high-throughput 16S rRNA sequencing and QIIME2 bioinformatics analyses.					
36872509	3	104	theme	multicompartmental	528:545	arg1	PT					555:556	PT	555:556	PT	555:556	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	104	theme	multicompartmental	528:545	arg1	injury					547:552	multicompartmental injury	528:552	multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures)	528:631	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
36872509	3	104	theme	multicompartmental	528:545	arg1	PT/CS					682:686	PT/CS	682:686	PT/CS	682:686	METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9-11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2-hours daily chronic restraint stress (PT/CS) or naive controls.					
37306238	14	0	theme	deformable	2046:2055	arg1	forms					2089:2093	deformable liposome-based cutaneous dosage forms	2046:2093	deformable liposome-based cutaneous dosage forms	2046:2093	Altogether, these results demonstrated that proliposomes may be a valuable intermediate for the preparation of deformable liposome-based cutaneous dosage forms, improving the stability without compromising the overall performances.					
37306238	9	1	theme	slurried	1256:1263	arg1	proliposomes					1266:1277	spray-dried and "slurried" proliposomes	1239:1277	spray-dried and "slurried" proliposomes	1239:1277	Based on the technological properties, sucrose and trehalose at 2:1 sugar/lipid ratio resulted in the best carriers for spray-dried and "slurried" proliposomes, respectively.					
37306238	12	2	dep	obtained	1732:1739	arg1	loading					1741:1747	loading	1741:1747	loading	1741:1747	The permeation through human epidermis of CBD carried by DiMiL systems was significantly improved compared to that obtained loading the drug in conventional deformable liposomes with the same lipid composition or in an oil solution.					
37306238	10	3	theme	systems	1465:1471	arg1	organization					1443:1454	the structural organization	1428:1454	the structural organization of DiMiL systems	1428:1471	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	12	4	theme	human	1640:1644	arg1	epidermis					1646:1654	human epidermis	1640:1654	human epidermis	1640:1654	The permeation through human epidermis of CBD carried by DiMiL systems was significantly improved compared to that obtained loading the drug in conventional deformable liposomes with the same lipid composition or in an oil solution.					
37306238	0	5	theme	Structural	89:98	arg1	Features					100:107	Structural Features	89:107	Structural Features	89:107	Micelles-in-Liposome Systems Obtained by Proliposomal Approach for Cannabidiol Delivery: Structural Features and Skin Penetration.					
37306238	9	6	theme	"	1264:1264	arg1	proliposomes					1266:1277	spray-dried and "slurried" proliposomes	1239:1277	spray-dried and "slurried" proliposomes	1239:1277	Based on the technological properties, sucrose and trehalose at 2:1 sugar/lipid ratio resulted in the best carriers for spray-dried and "slurried" proliposomes, respectively.					
37306238	5	7	theme	able	651:654	arg1	formulation					639:649	a formulation	637:649	a formulation able to enhance the skin penetration of cannabidiol (CBD)	637:707	In this work, we investigated the possible advantages of combining these two approaches to obtain a formulation able to enhance the skin penetration of cannabidiol (CBD).					
37306238	4	8	theme	hydrophobic	416:426	arg1	system					504:509	a drug-in-micelles-in-liposome system	473:509	a drug-in-micelles-in-liposome system (DiMiL)	473:517	As an alternative, a novel carrier, which encloses hydrophobic drugs in the inner core of vesicles, namely, a drug-in-micelles-in-liposome system (DiMiL), has been proposed.					
37306238	4	8	theme	hydrophobic	416:426	arg1	drugs					428:432	hydrophobic drugs	416:432	hydrophobic drugs in the inner core of vesicles	416:462	As an alternative, a novel carrier, which encloses hydrophobic drugs in the inner core of vesicles, namely, a drug-in-micelles-in-liposome system (DiMiL), has been proposed.					
37306238	1	9	theme	cutaneous	189:197	arg1	administration					199:212	cutaneous administration	189:212	cutaneous administration	189:212	Deformable liposomes represent valuable drug carriers for cutaneous administration.					
37306238	12	10	theme	CBD	1659:1661	arg1	permeation					1621:1630	The permeation	1617:1630	The permeation through human epidermis of CBD carried by DiMiL systems	1617:1686	The permeation through human epidermis of CBD carried by DiMiL systems was significantly improved compared to that obtained loading the drug in conventional deformable liposomes with the same lipid composition or in an oil solution.					
37306238	0	11	theme	Skin	113:116	arg1	Penetration					118:128	Skin Penetration	113:128	Skin Penetration	113:128	Micelles-in-Liposome Systems Obtained by Proliposomal Approach for Cannabidiol Delivery: Structural Features and Skin Penetration.					
37306238	9	12	theme	2:1	1183:1185	arg1	ratio					1199:1203	2:1 sugar/lipid ratio	1183:1203	2:1 sugar/lipid ratio	1183:1203	Based on the technological properties, sucrose and trehalose at 2:1 sugar/lipid ratio resulted in the best carriers for spray-dried and "slurried" proliposomes, respectively.					
37306238	8	13	theme	proliposomes	1027:1038	arg1	hydration					1014:1022	the hydration	1010:1022	the hydration of proliposomes with a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate)	1010:1116	DiMiL systems were extemporaneously obtained by the hydration of proliposomes with a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate).					
37306238	9	14	theme	spray-dried	1239:1249	arg1	proliposomes					1266:1277	spray-dried and "slurried" proliposomes	1239:1277	spray-dried and "slurried" proliposomes	1239:1277	Based on the technological properties, sucrose and trehalose at 2:1 sugar/lipid ratio resulted in the best carriers for spray-dried and "slurried" proliposomes, respectively.					
37306238	4	15	theme	drug-in-micelles-in-liposome	475:502	arg1	system					504:509	a drug-in-micelles-in-liposome system	473:509	a drug-in-micelles-in-liposome system (DiMiL)	473:517	As an alternative, a novel carrier, which encloses hydrophobic drugs in the inner core of vesicles, namely, a drug-in-micelles-in-liposome system (DiMiL), has been proposed.					
37306238	4	15	theme	drug-in-micelles-in-liposome	475:502	arg1	drugs					428:432	hydrophobic drugs	416:432	hydrophobic drugs in the inner core of vesicles	416:462	As an alternative, a novel carrier, which encloses hydrophobic drugs in the inner core of vesicles, namely, a drug-in-micelles-in-liposome system (DiMiL), has been proposed.					
37306238	4	15	theme	drug-in-micelles-in-liposome	475:502	arg1	DiMiL					512:516	DiMiL	512:516	DiMiL	512:516	As an alternative, a novel carrier, which encloses hydrophobic drugs in the inner core of vesicles, namely, a drug-in-micelles-in-liposome system (DiMiL), has been proposed.					
37306238	14	16	theme	dosage	2082:2087	arg1	forms					2089:2093	deformable liposome-based cutaneous dosage forms	2046:2093	deformable liposome-based cutaneous dosage forms	2046:2093	Altogether, these results demonstrated that proliposomes may be a valuable intermediate for the preparation of deformable liposome-based cutaneous dosage forms, improving the stability without compromising the overall performances.					
37306238	10	17	theme	aqueous	1356:1362	arg1	core					1364:1367	the aqueous core	1352:1367	the aqueous core of lipid vesicles	1352:1385	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	4	18	from	drugs	428:432	arg1	core					447:450	the inner core	437:450	the inner core of vesicles	437:462	As an alternative, a novel carrier, which encloses hydrophobic drugs in the inner core of vesicles, namely, a drug-in-micelles-in-liposome system (DiMiL), has been proposed.					
37306238	8	19	with	proliposomes	1027:1038	arg1	dispersion					1072:1081	a Kolliphor HS 15 micellar dispersion	1045:1081	a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate)	1045:1116	DiMiL systems were extemporaneously obtained by the hydration of proliposomes with a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate).					
37306238	11	20	theme	sugar	1610:1614	arg1	presence					1598:1605	the presence	1594:1605	the presence of sugar	1594:1614	All formulations were highly deformable and able to control CBD release regardless of the presence of sugar.					
37306238	10	21	theme	structural	1432:1441	arg1	organization					1443:1454	the structural organization	1428:1454	the structural organization of DiMiL systems	1428:1471	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	8	22	theme	DiMiL	962:966	arg1	systems					968:974	DiMiL systems	962:974	DiMiL systems	962:974	DiMiL systems were extemporaneously obtained by the hydration of proliposomes with a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate).					
37306238	12	23	theme	conventional	1761:1772	arg1	liposomes					1785:1793	conventional deformable liposomes	1761:1793	conventional deformable liposomes with the same lipid composition or in an oil solution	1761:1847	The permeation through human epidermis of CBD carried by DiMiL systems was significantly improved compared to that obtained loading the drug in conventional deformable liposomes with the same lipid composition or in an oil solution.					
37306238	14	24	theme	liposome-based	2057:2070	arg1	forms					2089:2093	deformable liposome-based cutaneous dosage forms	2046:2093	deformable liposome-based cutaneous dosage forms	2046:2093	Altogether, these results demonstrated that proliposomes may be a valuable intermediate for the preparation of deformable liposome-based cutaneous dosage forms, improving the stability without compromising the overall performances.					
37306238	13	25	theme	further	1898:1904	arg1	increase					1913:1920	a further slight increase	1896:1920	a further slight increase of the flux	1896:1932	Furthermore, the presence of trehalose led to a further slight increase of the flux.					
37306238	10	26	theme	SAXS	1493:1496	arg1	analyses					1498:1505	SAXS analyses	1493:1505	SAXS analyses	1493:1505	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	8	27	theme	HS	1057:1058	arg1	dispersion					1072:1081	a Kolliphor HS 15 micellar dispersion	1045:1081	a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate)	1045:1116	DiMiL systems were extemporaneously obtained by the hydration of proliposomes with a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate).					
37306238	4	28	theme	novel	386:390	arg1	alternative					371:381	an alternative	368:381	an alternative	368:381	As an alternative, a novel carrier, which encloses hydrophobic drugs in the inner core of vesicles, namely, a drug-in-micelles-in-liposome system (DiMiL), has been proposed.					
37306238	4	28	theme	novel	386:390	arg1	carrier					392:398	a novel carrier	384:398	a novel carrier	384:398	As an alternative, a novel carrier, which encloses hydrophobic drugs in the inner core of vesicles, namely, a drug-in-micelles-in-liposome system (DiMiL), has been proposed.					
37306238	8	29	theme	micellar	1063:1070	arg1	dispersion					1072:1081	a Kolliphor HS 15 micellar dispersion	1045:1081	a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate)	1045:1116	DiMiL systems were extemporaneously obtained by the hydration of proliposomes with a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate).					
37306238	12	30	with	liposomes	1785:1793	arg1	composition					1815:1825	the same lipid composition	1800:1825	the same lipid composition	1800:1825	The permeation through human epidermis of CBD carried by DiMiL systems was significantly improved compared to that obtained loading the drug in conventional deformable liposomes with the same lipid composition or in an oil solution.					
37306238	0	31	theme	Micelles-in-Liposome	0:19	arg1	Systems					21:27	Micelles-in-Liposome Systems	0:27	Micelles-in-Liposome Systems	0:27	Micelles-in-Liposome Systems Obtained by Proliposomal Approach for Cannabidiol Delivery: Structural Features and Skin Penetration.					
37306238	8	32	theme	Kolliphor	1047:1055	arg1	dispersion					1072:1081	a Kolliphor HS 15 micellar dispersion	1045:1081	a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate)	1045:1116	DiMiL systems were extemporaneously obtained by the hydration of proliposomes with a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate).					
37306238	5	33	theme	possible	573:580	arg1	advantages					582:591	the possible advantages	569:591	the possible advantages of combining these two approaches to obtain a formulation able to enhance the skin penetration of cannabidiol (CBD)	569:707	In this work, we investigated the possible advantages of combining these two approaches to obtain a formulation able to enhance the skin penetration of cannabidiol (CBD).					
37306238	6	34	theme	different	825:833	arg1	ratios					854:859	different sugar/lipid weight ratios	825:859	different sugar/lipid weight ratios	825:859	Proliposomes were prepared by spray-drying or slurry method testing lactose, sucrose, and trehalose as carriers at different sugar/lipid weight ratios.					
37306238	10	35	theme	Cryo-EM	1294:1300	arg1	images					1302:1307	Cryo-EM images	1294:1307	Cryo-EM images	1294:1307	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	10	36	theme	sugars	1407:1412	arg1	presence					1395:1402	the presence	1391:1402	the presence of sugars	1391:1412	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	14	37	theme	overall	2145:2151	arg1	performances					2153:2164	the overall performances	2141:2164	the overall performances	2141:2164	Altogether, these results demonstrated that proliposomes may be a valuable intermediate for the preparation of deformable liposome-based cutaneous dosage forms, improving the stability without compromising the overall performances.					
37306238	9	38	theme	sugar/lipid	1187:1197	arg1	ratio					1199:1203	2:1 sugar/lipid ratio	1183:1203	2:1 sugar/lipid ratio	1183:1203	Based on the technological properties, sucrose and trehalose at 2:1 sugar/lipid ratio resulted in the best carriers for spray-dried and "slurried" proliposomes, respectively.					
37306238	5	39	theme	cannabidiol	691:701	arg1	penetration					676:686	the skin penetration	667:686	the skin penetration of cannabidiol (CBD)	667:707	In this work, we investigated the possible advantages of combining these two approaches to obtain a formulation able to enhance the skin penetration of cannabidiol (CBD).					
37306238	10	40	theme	lipid	1372:1376	arg1	vesicles					1378:1385	lipid vesicles	1372:1385	lipid vesicles	1372:1385	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	12	41	from	drug	1753:1756	arg1	liposomes					1785:1793	conventional deformable liposomes	1761:1793	conventional deformable liposomes with the same lipid composition or in an oil solution	1761:1847	The permeation through human epidermis of CBD carried by DiMiL systems was significantly improved compared to that obtained loading the drug in conventional deformable liposomes with the same lipid composition or in an oil solution.					
37306238	6	42	from	ratios	854:859	arg1	lactose					778:784	lactose	778:784	lactose	778:784	Proliposomes were prepared by spray-drying or slurry method testing lactose, sucrose, and trehalose as carriers at different sugar/lipid weight ratios.					
37306238	6	42	from	ratios	854:859	arg1	carriers					813:820	carriers	813:820	carriers at different sugar/lipid weight ratios	813:859	Proliposomes were prepared by spray-drying or slurry method testing lactose, sucrose, and trehalose as carriers at different sugar/lipid weight ratios.					
37306238	6	42	from	ratios	854:859	arg1	sucrose					787:793	sucrose	787:793	sucrose	787:793	Proliposomes were prepared by spray-drying or slurry method testing lactose, sucrose, and trehalose as carriers at different sugar/lipid weight ratios.					
37306238	6	42	from	ratios	854:859	arg1	trehalose					800:808	trehalose	800:808	trehalose	800:808	Proliposomes were prepared by spray-drying or slurry method testing lactose, sucrose, and trehalose as carriers at different sugar/lipid weight ratios.					
37306238	10	43	attach	presence	1328:1335	arg1	core					1364:1367	the aqueous core	1352:1367	the aqueous core of lipid vesicles	1352:1385	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	10	43	attach	presence	1328:1335	arg2	micelles					1340:1347	micelles	1340:1347	micelles	1340:1347	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	10	44	theme	DiMiL	1459:1463	arg1	systems					1465:1471	DiMiL systems	1459:1471	DiMiL systems	1459:1471	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	9	45	theme	best	1221:1224	arg1	carriers					1226:1233	the best carriers	1217:1233	the best carriers for spray-dried and "slurried" proliposomes	1217:1277	Based on the technological properties, sucrose and trehalose at 2:1 sugar/lipid ratio resulted in the best carriers for spray-dried and "slurried" proliposomes, respectively.					
37306238	7	46	from	85:15	951:955	arg1	fixed					942:946	fixed	942:946	fixed	942:946	The ratio between soy-phosphatidylcholine (main lipid) and Tween 80 was instead fixed at 85:15 w/w.					
37306238	7	46	from	85:15	951:955	arg1	ratio					866:870	The ratio	862:870	The ratio between soy-phosphatidylcholine (main lipid) and Tween 80	862:928	The ratio between soy-phosphatidylcholine (main lipid) and Tween 80 was instead fixed at 85:15 w/w.					
37306238	10	47	from	presence	1328:1335	arg1	core					1364:1367	the aqueous core	1352:1367	the aqueous core of lipid vesicles	1352:1385	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	4	48	theme	vesicles	455:462	arg1	core					447:450	the inner core	437:450	the inner core of vesicles	437:462	As an alternative, a novel carrier, which encloses hydrophobic drugs in the inner core of vesicles, namely, a drug-in-micelles-in-liposome system (DiMiL), has been proposed.					
37306238	2	49	theme	drug	268:271	arg1	leakage					273:279	the drug leakage	264:279	the drug leakage	264:279	Nevertheless, the fluid lipid membrane can favor the drug leakage during storage.					
37306238	12	50	theme	DiMiL	1674:1678	arg1	systems					1680:1686	DiMiL systems	1674:1686	DiMiL systems	1674:1686	The permeation through human epidermis of CBD carried by DiMiL systems was significantly improved compared to that obtained loading the drug in conventional deformable liposomes with the same lipid composition or in an oil solution.					
37306238	0	51	theme	Proliposomal	41:52	arg1	Approach					54:61	Proliposomal Approach	41:61	Proliposomal Approach for Cannabidiol Delivery	41:86	Micelles-in-Liposome Systems Obtained by Proliposomal Approach for Cannabidiol Delivery: Structural Features and Skin Penetration.					
37306238	12	52	theme	oil	1836:1838	arg1	solution					1840:1847	an oil solution	1833:1847	an oil solution	1833:1847	The permeation through human epidermis of CBD carried by DiMiL systems was significantly improved compared to that obtained loading the drug in conventional deformable liposomes with the same lipid composition or in an oil solution.					
37306238	6	53	theme	weight	847:852	arg1	ratios					854:859	different sugar/lipid weight ratios	825:859	different sugar/lipid weight ratios	825:859	Proliposomes were prepared by spray-drying or slurry method testing lactose, sucrose, and trehalose as carriers at different sugar/lipid weight ratios.					
37306238	6	54	theme	slurry	756:761	arg1	method					763:768	spray-drying or slurry method	740:768	spray-drying or slurry method testing lactose, sucrose, and trehalose as carriers at different sugar/lipid weight ratios	740:859	Proliposomes were prepared by spray-drying or slurry method testing lactose, sucrose, and trehalose as carriers at different sugar/lipid weight ratios.					
37306238	0	55	theme	Cannabidiol	67:77	arg1	Delivery					79:86	Cannabidiol Delivery	67:86	Cannabidiol Delivery	67:86	Micelles-in-Liposome Systems Obtained by Proliposomal Approach for Cannabidiol Delivery: Structural Features and Skin Penetration.					
37306238	12	56	theme	lipid	1809:1813	arg1	composition					1815:1825	the same lipid composition	1800:1825	the same lipid composition	1800:1825	The permeation through human epidermis of CBD carried by DiMiL systems was significantly improved compared to that obtained loading the drug in conventional deformable liposomes with the same lipid composition or in an oil solution.					
37306238	6	57	theme	sugar/lipid	835:845	arg1	ratios					854:859	different sugar/lipid weight ratios	825:859	different sugar/lipid weight ratios	825:859	Proliposomes were prepared by spray-drying or slurry method testing lactose, sucrose, and trehalose as carriers at different sugar/lipid weight ratios.					
37306238	12	58	theme	deformable	1774:1783	arg1	liposomes					1785:1793	conventional deformable liposomes	1761:1793	conventional deformable liposomes with the same lipid composition or in an oil solution	1761:1847	The permeation through human epidermis of CBD carried by DiMiL systems was significantly improved compared to that obtained loading the drug in conventional deformable liposomes with the same lipid composition or in an oil solution.					
37306238	1	59	theme	Deformable	131:140	arg1	liposomes					142:150	Deformable liposomes	131:150	Deformable liposomes	131:150	Deformable liposomes represent valuable drug carriers for cutaneous administration.					
37306238	5	60	theme	skin	671:674	arg1	penetration					676:686	the skin penetration	667:686	the skin penetration of cannabidiol (CBD)	667:707	In this work, we investigated the possible advantages of combining these two approaches to obtain a formulation able to enhance the skin penetration of cannabidiol (CBD).					
37306238	4	61	theme	inner	441:445	arg1	core					447:450	the inner core	437:450	the inner core of vesicles	437:462	As an alternative, a novel carrier, which encloses hydrophobic drugs in the inner core of vesicles, namely, a drug-in-micelles-in-liposome system (DiMiL), has been proposed.					
37306238	10	62	theme	vesicles	1378:1385	arg1	core					1364:1367	the aqueous core	1352:1367	the aqueous core of lipid vesicles	1352:1385	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	6	63	theme	spray-drying	740:751	arg1	method					763:768	spray-drying or slurry method	740:768	spray-drying or slurry method testing lactose, sucrose, and trehalose as carriers at different sugar/lipid weight ratios	740:859	Proliposomes were prepared by spray-drying or slurry method testing lactose, sucrose, and trehalose as carriers at different sugar/lipid weight ratios.					
37306238	9	64	from	ratio	1199:1203	arg1	trehalose					1170:1178	trehalose	1170:1178	trehalose	1170:1178	Based on the technological properties, sucrose and trehalose at 2:1 sugar/lipid ratio resulted in the best carriers for spray-dried and "slurried" proliposomes, respectively.					
37306238	9	64	from	ratio	1199:1203	arg1	sucrose					1158:1164	sucrose	1158:1164	sucrose	1158:1164	Based on the technological properties, sucrose and trehalose at 2:1 sugar/lipid ratio resulted in the best carriers for spray-dried and "slurried" proliposomes, respectively.					
37306238	13	65	theme	slight	1906:1911	arg1	increase					1913:1920	a further slight increase	1896:1920	a further slight increase of the flux	1896:1932	Furthermore, the presence of trehalose led to a further slight increase of the flux.					
37306238	3	66	theme	suitable	326:333	arg1	strategy					335:342	a suitable strategy	324:342	a suitable strategy to solve this issue	324:362	Proliposomes may represent a suitable strategy to solve this issue.					
37306238	2	67	theme	lipid	239:243	arg1	membrane					245:252	the fluid lipid membrane	229:252	the fluid lipid membrane	229:252	Nevertheless, the fluid lipid membrane can favor the drug leakage during storage.					
37306238	14	68	theme	valuable	2001:2008	arg1	intermediate					2010:2021	a valuable intermediate	1999:2021	a valuable intermediate for the preparation of deformable liposome-based cutaneous dosage forms	1999:2093	Altogether, these results demonstrated that proliposomes may be a valuable intermediate for the preparation of deformable liposome-based cutaneous dosage forms, improving the stability without compromising the overall performances.					
37306238	14	68	theme	valuable	2001:2008	arg1	proliposomes					1979:1990	proliposomes	1979:1990	proliposomes	1979:1990	Altogether, these results demonstrated that proliposomes may be a valuable intermediate for the preparation of deformable liposome-based cutaneous dosage forms, improving the stability without compromising the overall performances.					
37306238	14	69	theme	forms	2089:2093	arg1	preparation					2031:2041	the preparation	2027:2041	the preparation of deformable liposome-based cutaneous dosage forms	2027:2093	Altogether, these results demonstrated that proliposomes may be a valuable intermediate for the preparation of deformable liposome-based cutaneous dosage forms, improving the stability without compromising the overall performances.					
37306238	8	70	contain	containing	1084:1093	arg1	dispersion					1072:1081	a Kolliphor HS 15 micellar dispersion	1045:1081	a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate)	1045:1116	DiMiL systems were extemporaneously obtained by the hydration of proliposomes with a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate).					
37306238	8	70	contain	containing	1084:1093	arg2	CBD					1095:1097	CBD	1095:1097	CBD	1095:1097	DiMiL systems were extemporaneously obtained by the hydration of proliposomes with a Kolliphor HS 15 micellar dispersion (containing CBD, when appropriate).					
37306238	11	71	theme	CBD	1568:1570	arg1	release					1572:1578	CBD release	1568:1578	CBD release	1568:1578	All formulations were highly deformable and able to control CBD release regardless of the presence of sugar.					
37306238	2	72	theme	fluid	233:237	arg1	membrane					245:252	the fluid lipid membrane	229:252	the fluid lipid membrane	229:252	Nevertheless, the fluid lipid membrane can favor the drug leakage during storage.					
37306238	1	73	theme	valuable	162:169	arg1	carriers					176:183	valuable drug carriers	162:183	valuable drug carriers for cutaneous administration	162:212	Deformable liposomes represent valuable drug carriers for cutaneous administration.					
37306238	13	74	theme	trehalose	1879:1887	arg1	presence					1867:1874	the presence	1863:1874	the presence of trehalose	1863:1887	Furthermore, the presence of trehalose led to a further slight increase of the flux.					
37306238	12	75	from	liposomes	1785:1793	arg1	solution					1840:1847	an oil solution	1833:1847	an oil solution	1833:1847	The permeation through human epidermis of CBD carried by DiMiL systems was significantly improved compared to that obtained loading the drug in conventional deformable liposomes with the same lipid composition or in an oil solution.					
37306238	12	76	theme	same	1804:1807	arg1	composition					1815:1825	the same lipid composition	1800:1825	the same lipid composition	1800:1825	The permeation through human epidermis of CBD carried by DiMiL systems was significantly improved compared to that obtained loading the drug in conventional deformable liposomes with the same lipid composition or in an oil solution.					
37306238	13	77	theme	flux	1929:1932	arg1	increase					1913:1920	a further slight increase	1896:1920	a further slight increase of the flux	1896:1932	Furthermore, the presence of trehalose led to a further slight increase of the flux.					
37306238	10	78	theme	micelles	1340:1347	arg1	presence					1328:1335	the presence	1324:1335	the presence of micelles in the aqueous core of lipid vesicles	1324:1385	Cryo-EM images clearly showed the presence of micelles in the aqueous core of lipid vesicles and the presence of sugars did not alter the structural organization of DiMiL systems, as demonstrated by SAXS analyses.					
37306238	1	79	theme	drug	171:174	arg1	carriers					176:183	valuable drug carriers	162:183	valuable drug carriers for cutaneous administration	162:212	Deformable liposomes represent valuable drug carriers for cutaneous administration.					
37306238	7	80	theme	main	905:908	arg1	soy-phosphatidylcholine					880:902	soy-phosphatidylcholine	880:902	soy-phosphatidylcholine (main lipid)	880:915	The ratio between soy-phosphatidylcholine (main lipid) and Tween 80 was instead fixed at 85:15 w/w.					
37306238	7	80	theme	main	905:908	arg1	lipid					910:914	main lipid	905:914	main lipid	905:914	The ratio between soy-phosphatidylcholine (main lipid) and Tween 80 was instead fixed at 85:15 w/w.					
37306238	9	81	theme	technological	1132:1144	arg1	properties					1146:1155	the technological properties	1128:1155	the technological properties	1128:1155	Based on the technological properties, sucrose and trehalose at 2:1 sugar/lipid ratio resulted in the best carriers for spray-dried and "slurried" proliposomes, respectively.					
37306238	14	82	theme	cutaneous	2072:2080	arg1	forms					2089:2093	deformable liposome-based cutaneous dosage forms	2046:2093	deformable liposome-based cutaneous dosage forms	2046:2093	Altogether, these results demonstrated that proliposomes may be a valuable intermediate for the preparation of deformable liposome-based cutaneous dosage forms, improving the stability without compromising the overall performances.					
37240233	4	0	theme	benoxacor	655:663	arg1	enantiomers					665:675	the two benoxacor enantiomers	647:675	the two benoxacor enantiomers	647:675	Under normal-phase conditions, the two benoxacor enantiomers were completely separated on Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns and partially separated on a Lux Cellulose-2 column.					
37240233	1	1	theme	normal-phase	307:318	arg1	conditions					339:348	normal-phase and reversed-phase conditions	307:348	normal-phase and reversed-phase conditions	307:348	The separation of benoxacor enantiomers on six commercial chiral columns was investigated by high-performance liquid chromatography (HPLC) under normal-phase and reversed-phase conditions.					
37240233	4	2	theme	IC	730:731	arg1	columns					771:777	Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns	706:777	Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns	706:777	Under normal-phase conditions, the two benoxacor enantiomers were completely separated on Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns and partially separated on a Lux Cellulose-2 column.					
37240233	7	3	theme	enthalpy	1175:1182	arg1	results					1206:1212	the enthalpy (ΔH) and entropy (ΔS) results	1171:1212	the enthalpy (ΔH) and entropy (ΔS) results	1171:1212	As the column temperature increased from 10 °C to 4 °C, the enthalpy (ΔH) and entropy (ΔS) results indicated that the resolution was strongly affected by the temperature and that the lowest temperature did not always produce the best resolution.					
37240233	3	4	theme	enantiomers	589:599	arg1	separation					565:574	the separation	561:574	the separation of benoxacor enantiomers	561:599	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers were examined.					
37240233	4	5	theme	Lux	734:736	arg1	Cellulose-1					738:748	Lux Cellulose-1	734:748	Lux Cellulose-1	734:748	Under normal-phase conditions, the two benoxacor enantiomers were completely separated on Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns and partially separated on a Lux Cellulose-2 column.					
37240233	8	6	from	solvents	1488:1495	arg1	stability					1450:1458	the stability	1446:1458	the stability of benoxacor enantiomers in solvents	1446:1495	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	8	6	from	solvents	1488:1495	arg1	degradation					1505:1515	the degradation	1501:1515	the degradation of benoxacor enantiomers in three types of horticultural soil	1501:1577	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	1	7	theme	chiral	220:225	arg1	columns					227:233	six commercial chiral columns	205:233	six commercial chiral columns	205:233	The separation of benoxacor enantiomers on six commercial chiral columns was investigated by high-performance liquid chromatography (HPLC) under normal-phase and reversed-phase conditions.					
37240233	9	8	theme	=	1735:1735	arg1	water					1725:1729	water	1725:1729	water (pH = 4.0, 7.0, and 9.0)	1725:1754	Benoxacor enantiomers were stable, and degradation or racemization were not observed in methanol, ethanol, isopropanol, acetonitrile, hexane, or water (pH = 4.0, 7.0, and 9.0).					
37240233	9	8	theme	=	1735:1735	arg1	pH					1732:1733	pH = 4.0, 7.0, and 9.0	1732:1753	pH = 4.0, 7.0, and 9.0	1732:1753	Benoxacor enantiomers were stable, and degradation or racemization were not observed in methanol, ethanol, isopropanol, acetonitrile, hexane, or water (pH = 4.0, 7.0, and 9.0).					
37240233	4	9	theme	Chiralpak	706:714	arg1	AD					716:717	Chiralpak AD	706:717	Chiralpak AD	706:717	Under normal-phase conditions, the two benoxacor enantiomers were completely separated on Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns and partially separated on a Lux Cellulose-2 column.					
37240233	0	10	theme	Liquid	139:144	arg1	Chromatography					146:159	Reversed-Phase High Performance Liquid Chromatography	107:159	Reversed-Phase High Performance Liquid Chromatography	107:159	Enantiomeric Separation and Degradation of Benoxacor Enantiomers in Horticultural Soil by Normal-Phase and Reversed-Phase High Performance Liquid Chromatography.					
37240233	8	11	from	enantiomers	1473:1483	arg1	solvents					1488:1495	solvents	1488:1495	solvents	1488:1495	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	7	12	theme	°C	1159:1160	arg1	°C					1167:1168	10 °C to 4 °C	1156:1168	10 °C to 4 °C	1156:1168	As the column temperature increased from 10 °C to 4 °C, the enthalpy (ΔH) and entropy (ΔS) results indicated that the resolution was strongly affected by the temperature and that the lowest temperature did not always produce the best resolution.					
37240233	10	13	theme	S-benoxacor	1811:1821	arg1	rate					1803:1806	the degradation rate	1787:1806	the degradation rate of S-benoxacor	1787:1821	In three horticultural soils, the degradation rate of S-benoxacor was faster than that of R-benoxacor, resulting in soil enrichment with R-benoxacor.					
37240233	10	13	theme	S-benoxacor	1811:1821	arg1	faster					1827:1832	faster	1827:1832	faster	1827:1832	In three horticultural soils, the degradation rate of S-benoxacor was faster than that of R-benoxacor, resulting in soil enrichment with R-benoxacor.					
37240233	5	14	theme	Cellulose-3	922:932	arg1	column					934:939	a Lux Cellulose-3 column	916:939	a Lux Cellulose-3 column	916:939	Under reversed-phase conditions, benoxacor enantiomers were completely separated on a Lux Cellulose-3 column and partially separated on Chiralpak IC and Lux Cellulose-1 columns.					
37240233	5	15	theme	Cellulose-1	989:999	arg1	columns					1001:1007	Chiralpak IC and Lux Cellulose-1 columns	968:1007	columns	1001:1007	Under reversed-phase conditions, benoxacor enantiomers were completely separated on a Lux Cellulose-3 column and partially separated on Chiralpak IC and Lux Cellulose-1 columns.					
37240233	6	16	theme	Normal-phase	1010:1021	arg1	HPLC					1023:1026	Normal-phase HPLC	1010:1026	Normal-phase HPLC	1010:1026	Normal-phase HPLC performed better than reversed-phase HPLC for the separation of benoxacor enantiomers.					
37240233	8	17	theme	benoxacor	1520:1528	arg1	enantiomers					1530:1540	benoxacor enantiomers	1520:1540	benoxacor enantiomers in three types of horticultural soil	1520:1577	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	11	18	theme	levels	1988:1993	arg1	assessment					1963:1972	the risk assessment	1954:1972	the risk assessment of enantiomer levels of benoxacor in the environment	1954:2025	The results of this study will help to improve the risk assessment of enantiomer levels of benoxacor in the environment.					
37240233	8	19	theme	optimized	1364:1372	arg1	method					1385:1390	An optimized separation method	1361:1390	An optimized separation method on the Lux Cellulose-3 column	1361:1420	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	8	20	from	degradation	1505:1515	arg1	types					1551:1555	three types	1545:1555	three types of horticultural soil	1545:1577	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	8	20	from	degradation	1505:1515	arg1	solvents					1488:1495	solvents	1488:1495	solvents	1488:1495	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	5	21	theme	Chiralpak	968:976	arg1	IC					978:979	Chiralpak IC and Lux Cellulose-1 columns	968:1007	IC	978:979	Under reversed-phase conditions, benoxacor enantiomers were completely separated on a Lux Cellulose-3 column and partially separated on Chiralpak IC and Lux Cellulose-1 columns.					
37240233	6	22	theme	enantiomers	1102:1112	arg1	separation					1078:1087	the separation	1074:1087	the separation of benoxacor enantiomers	1074:1112	Normal-phase HPLC performed better than reversed-phase HPLC for the separation of benoxacor enantiomers.					
37240233	4	23	theme	Lux	755:757	arg1	Cellulose-3					759:769	Lux Cellulose-3	755:769	Lux Cellulose-3	755:769	Under normal-phase conditions, the two benoxacor enantiomers were completely separated on Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns and partially separated on a Lux Cellulose-2 column.					
37240233	1	24	theme	enantiomers	190:200	arg1	separation					166:175	The separation	162:175	The separation of benoxacor enantiomers on six commercial chiral columns	162:233	The separation of benoxacor enantiomers on six commercial chiral columns was investigated by high-performance liquid chromatography (HPLC) under normal-phase and reversed-phase conditions.					
37240233	8	25	theme	horticultural	1560:1572	arg1	soil					1574:1577	horticultural soil	1560:1577	horticultural soil	1560:1577	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	1	26	from	separation	166:175	arg1	columns					227:233	six commercial chiral columns	205:233	six commercial chiral columns	205:233	The separation of benoxacor enantiomers on six commercial chiral columns was investigated by high-performance liquid chromatography (HPLC) under normal-phase and reversed-phase conditions.					
37240233	8	27	theme	Cellulose-3	1403:1413	arg1	column					1415:1420	the Lux Cellulose-3 column	1395:1420	the Lux Cellulose-3 column	1395:1420	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	0	28	theme	High	122:125	arg1	Chromatography					146:159	Reversed-Phase High Performance Liquid Chromatography	107:159	Reversed-Phase High Performance Liquid Chromatography	107:159	Enantiomeric Separation and Degradation of Benoxacor Enantiomers in Horticultural Soil by Normal-Phase and Reversed-Phase High Performance Liquid Chromatography.					
37240233	3	29	from	effects	458:464	arg1	separation					565:574	the separation	561:574	the separation of benoxacor enantiomers	561:599	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers were examined.					
37240233	5	30	theme	reversed-phase	838:851	arg1	conditions					853:862	reversed-phase conditions	838:862	reversed-phase conditions	838:862	Under reversed-phase conditions, benoxacor enantiomers were completely separated on a Lux Cellulose-3 column and partially separated on Chiralpak IC and Lux Cellulose-1 columns.					
37240233	8	31	from	method	1385:1390	arg1	column					1415:1420	the Lux Cellulose-3 column	1395:1420	the Lux Cellulose-3 column	1395:1420	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	0	32	theme	Enantiomeric	0:11	arg1	Separation					13:22	Enantiomeric Separation	0:22	Enantiomeric Separation	0:22	Enantiomeric Separation and Degradation of Benoxacor Enantiomers in Horticultural Soil by Normal-Phase and Reversed-Phase High Performance Liquid Chromatography.					
37240233	4	33	theme	Lux	808:810	arg1	column					824:829	a Lux Cellulose-2 column	806:829	a Lux Cellulose-2 column	806:829	Under normal-phase conditions, the two benoxacor enantiomers were completely separated on Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns and partially separated on a Lux Cellulose-2 column.					
37240233	8	34	used	used	1426:1429	arg2	method					1385:1390	An optimized separation method	1361:1390	An optimized separation method on the Lux Cellulose-3 column	1361:1420	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	8	34	used	used	1426:1429	arg2	column					1415:1420	the Lux Cellulose-3 column	1395:1420	the Lux Cellulose-3 column	1395:1420	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	8	35	from	types	1551:1555	arg1	stability					1450:1458	the stability	1446:1458	the stability of benoxacor enantiomers in solvents	1446:1495	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	8	35	from	types	1551:1555	arg1	degradation					1505:1515	the degradation	1501:1515	the degradation of benoxacor enantiomers in three types of horticultural soil	1501:1577	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	3	36	theme	stationary	480:489	arg1	CSPs					499:502	CSPs	499:502	CSPs	499:502	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers were examined.					
37240233	3	36	theme	stationary	480:489	arg1	phases					491:496	the chiral stationary phases	469:496	the chiral stationary phases (CSPs)	469:503	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers were examined.					
37240233	4	37	theme	normal-phase	622:633	arg1	conditions					635:644	normal-phase conditions	622:644	normal-phase conditions	622:644	Under normal-phase conditions, the two benoxacor enantiomers were completely separated on Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns and partially separated on a Lux Cellulose-2 column.					
37240233	7	38	theme	lowest	1298:1303	arg1	temperature					1305:1315	the lowest temperature	1294:1315	the lowest temperature	1294:1315	As the column temperature increased from 10 °C to 4 °C, the enthalpy (ΔH) and entropy (ΔS) results indicated that the resolution was strongly affected by the temperature and that the lowest temperature did not always produce the best resolution.					
37240233	3	39	theme	temperature	506:516	arg1	effects					458:464	The effects	454:464	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers	454:599	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers were examined.					
37240233	7	40	theme	best	1344:1347	arg1	resolution					1349:1358	the best resolution	1340:1358	the best resolution	1340:1358	As the column temperature increased from 10 °C to 4 °C, the enthalpy (ΔH) and entropy (ΔS) results indicated that the resolution was strongly affected by the temperature and that the lowest temperature did not always produce the best resolution.					
37240233	0	41	theme	Horticultural	68:80	arg1	Soil					82:85	Horticultural Soil	68:85	Horticultural Soil	68:85	Enantiomeric Separation and Degradation of Benoxacor Enantiomers in Horticultural Soil by Normal-Phase and Reversed-Phase High Performance Liquid Chromatography.					
37240233	5	42	theme	benoxacor	865:873	arg1	enantiomers					875:885	benoxacor enantiomers	865:885	benoxacor enantiomers	865:885	Under reversed-phase conditions, benoxacor enantiomers were completely separated on a Lux Cellulose-3 column and partially separated on Chiralpak IC and Lux Cellulose-1 columns.					
37240233	8	43	theme	enantiomers	1473:1483	arg1	stability					1450:1458	the stability	1446:1458	the stability of benoxacor enantiomers in solvents	1446:1495	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	8	43	theme	enantiomers	1473:1483	arg1	degradation					1505:1515	the degradation	1501:1515	the degradation of benoxacor enantiomers in three types of horticultural soil	1501:1577	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	3	44	theme	phase	530:534	arg1	composition					536:546	mobile phase composition	523:546	mobile phase composition	523:546	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers were examined.					
37240233	1	45	theme	high-performance	255:270	arg1	chromatography					279:292	high-performance liquid chromatography	255:292	high-performance liquid chromatography (HPLC)	255:299	The separation of benoxacor enantiomers on six commercial chiral columns was investigated by high-performance liquid chromatography (HPLC) under normal-phase and reversed-phase conditions.					
37240233	1	45	theme	high-performance	255:270	arg1	HPLC					295:298	HPLC	295:298	HPLC	295:298	The separation of benoxacor enantiomers on six commercial chiral columns was investigated by high-performance liquid chromatography (HPLC) under normal-phase and reversed-phase conditions.					
37240233	11	46	theme	benoxacor	1998:2006	arg1	levels					1988:1993	enantiomer levels	1977:1993	enantiomer levels of benoxacor	1977:2006	The results of this study will help to improve the risk assessment of enantiomer levels of benoxacor in the environment.					
37240233	9	47	theme	Benoxacor	1580:1588	arg1	enantiomers					1590:1600	Benoxacor enantiomers	1580:1600	Benoxacor enantiomers	1580:1600	Benoxacor enantiomers were stable, and degradation or racemization were not observed in methanol, ethanol, isopropanol, acetonitrile, hexane, or water (pH = 4.0, 7.0, and 9.0).					
37240233	10	48	theme	soil	1873:1876	arg1	enrichment					1878:1887	soil enrichment	1873:1887	soil enrichment with R-benoxacor	1873:1904	In three horticultural soils, the degradation rate of S-benoxacor was faster than that of R-benoxacor, resulting in soil enrichment with R-benoxacor.					
37240233	10	49	theme	horticultural	1766:1778	arg1	soils					1780:1784	three horticultural soils	1760:1784	three horticultural soils	1760:1784	In three horticultural soils, the degradation rate of S-benoxacor was faster than that of R-benoxacor, resulting in soil enrichment with R-benoxacor.					
37240233	11	50	from	assessment	1963:1972	arg1	environment					2015:2025	the environment	2011:2025	the environment	2011:2025	The results of this study will help to improve the risk assessment of enantiomer levels of benoxacor in the environment.					
37240233	3	51	theme	benoxacor	579:587	arg1	enantiomers					589:599	benoxacor enantiomers	579:599	benoxacor enantiomers	579:599	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers were examined.					
37240233	8	52	from	stability	1450:1458	arg1	types					1551:1555	three types	1545:1555	three types of horticultural soil	1545:1577	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	8	52	from	stability	1450:1458	arg1	solvents					1488:1495	solvents	1488:1495	solvents	1488:1495	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	0	53	theme	Enantiomers	53:63	arg1	Degradation					28:38	Degradation	28:38	Degradation	28:38	Enantiomeric Separation and Degradation of Benoxacor Enantiomers in Horticultural Soil by Normal-Phase and Reversed-Phase High Performance Liquid Chromatography.					
37240233	0	53	theme	Enantiomers	53:63	arg1	Separation					13:22	Enantiomeric Separation	0:22	Enantiomeric Separation	0:22	Enantiomeric Separation and Degradation of Benoxacor Enantiomers in Horticultural Soil by Normal-Phase and Reversed-Phase High Performance Liquid Chromatography.					
37240233	4	54	theme	Chiralpak	720:728	arg1	IC					730:731	Chiralpak IC	720:731	Chiralpak IC	720:731	Under normal-phase conditions, the two benoxacor enantiomers were completely separated on Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns and partially separated on a Lux Cellulose-2 column.					
37240233	10	55	theme	degradation	1791:1801	arg1	rate					1803:1806	the degradation rate	1787:1806	the degradation rate of S-benoxacor	1787:1821	In three horticultural soils, the degradation rate of S-benoxacor was faster than that of R-benoxacor, resulting in soil enrichment with R-benoxacor.					
37240233	10	55	theme	degradation	1791:1801	arg1	faster					1827:1832	faster	1827:1832	faster	1827:1832	In three horticultural soils, the degradation rate of S-benoxacor was faster than that of R-benoxacor, resulting in soil enrichment with R-benoxacor.					
37240233	1	56	theme	commercial	209:218	arg1	columns					227:233	six commercial chiral columns	205:233	six commercial chiral columns	205:233	The separation of benoxacor enantiomers on six commercial chiral columns was investigated by high-performance liquid chromatography (HPLC) under normal-phase and reversed-phase conditions.					
37240233	8	57	theme	soil	1574:1577	arg1	types					1551:1555	three types	1545:1555	three types of horticultural soil	1545:1577	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	2	58	theme	mobile	355:360	arg1	phases					362:367	The mobile phases	351:367	The mobile phases	351:367	The mobile phases included hexane/ethanol, hexane/isopropanol, acetonitrile/water, and methanol/water.					
37240233	6	59	theme	reversed-phase	1050:1063	arg1	HPLC					1065:1068	reversed-phase HPLC	1050:1068	reversed-phase HPLC	1050:1068	Normal-phase HPLC performed better than reversed-phase HPLC for the separation of benoxacor enantiomers.					
37240233	7	60	dep	°C	1167:1168	arg1	to					1162:1163	to	1162:1163	to	1162:1163	As the column temperature increased from 10 °C to 4 °C, the enthalpy (ΔH) and entropy (ΔS) results indicated that the resolution was strongly affected by the temperature and that the lowest temperature did not always produce the best resolution.					
37240233	5	61	theme	Lux	918:920	arg1	column					934:939	a Lux Cellulose-3 column	916:939	a Lux Cellulose-3 column	916:939	Under reversed-phase conditions, benoxacor enantiomers were completely separated on a Lux Cellulose-3 column and partially separated on Chiralpak IC and Lux Cellulose-1 columns.					
37240233	4	62	theme	AD	716:717	arg1	columns					771:777	Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns	706:777	Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns	706:777	Under normal-phase conditions, the two benoxacor enantiomers were completely separated on Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns and partially separated on a Lux Cellulose-2 column.					
37240233	5	63	theme	Lux	985:987	arg1	columns					1001:1007	Chiralpak IC and Lux Cellulose-1 columns	968:1007	columns	1001:1007	Under reversed-phase conditions, benoxacor enantiomers were completely separated on a Lux Cellulose-3 column and partially separated on Chiralpak IC and Lux Cellulose-1 columns.					
37240233	8	64	theme	enantiomers	1530:1540	arg1	stability					1450:1458	the stability	1446:1458	the stability of benoxacor enantiomers in solvents	1446:1495	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	8	64	theme	enantiomers	1530:1540	arg1	degradation					1505:1515	the degradation	1501:1515	the degradation of benoxacor enantiomers in three types of horticultural soil	1501:1577	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	4	65	theme	Cellulose-3	759:769	arg1	columns					771:777	Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns	706:777	Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns	706:777	Under normal-phase conditions, the two benoxacor enantiomers were completely separated on Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns and partially separated on a Lux Cellulose-2 column.					
37240233	7	66	theme	column	1122:1127	arg1	temperature					1129:1139	the column temperature	1118:1139	the column temperature	1118:1139	As the column temperature increased from 10 °C to 4 °C, the enthalpy (ΔH) and entropy (ΔS) results indicated that the resolution was strongly affected by the temperature and that the lowest temperature did not always produce the best resolution.					
37240233	11	67	theme	enantiomer	1977:1986	arg1	levels					1988:1993	enantiomer levels	1977:1993	enantiomer levels of benoxacor	1977:2006	The results of this study will help to improve the risk assessment of enantiomer levels of benoxacor in the environment.					
37240233	0	68	from	Degradation	28:38	arg1	Soil					82:85	Horticultural Soil	68:85	Horticultural Soil	68:85	Enantiomeric Separation and Degradation of Benoxacor Enantiomers in Horticultural Soil by Normal-Phase and Reversed-Phase High Performance Liquid Chromatography.					
37240233	4	69	theme	Cellulose-1	738:748	arg1	columns					771:777	Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns	706:777	Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns	706:777	Under normal-phase conditions, the two benoxacor enantiomers were completely separated on Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns and partially separated on a Lux Cellulose-2 column.					
37240233	1	70	theme	benoxacor	180:188	arg1	enantiomers					190:200	benoxacor enantiomers	180:200	benoxacor enantiomers	180:200	The separation of benoxacor enantiomers on six commercial chiral columns was investigated by high-performance liquid chromatography (HPLC) under normal-phase and reversed-phase conditions.					
37240233	6	71	theme	benoxacor	1092:1100	arg1	enantiomers					1102:1112	benoxacor enantiomers	1092:1112	benoxacor enantiomers	1092:1112	Normal-phase HPLC performed better than reversed-phase HPLC for the separation of benoxacor enantiomers.					
37240233	0	72	from	Separation	13:22	arg1	Soil					82:85	Horticultural Soil	68:85	Horticultural Soil	68:85	Enantiomeric Separation and Degradation of Benoxacor Enantiomers in Horticultural Soil by Normal-Phase and Reversed-Phase High Performance Liquid Chromatography.					
37240233	8	73	theme	separation	1374:1383	arg1	method					1385:1390	An optimized separation method	1361:1390	An optimized separation method on the Lux Cellulose-3 column	1361:1420	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	0	74	theme	Reversed-Phase	107:120	arg1	Chromatography					146:159	Reversed-Phase High Performance Liquid Chromatography	107:159	Reversed-Phase High Performance Liquid Chromatography	107:159	Enantiomeric Separation and Degradation of Benoxacor Enantiomers in Horticultural Soil by Normal-Phase and Reversed-Phase High Performance Liquid Chromatography.					
37240233	4	75	theme	Cellulose-2	812:822	arg1	column					824:829	a Lux Cellulose-2 column	806:829	a Lux Cellulose-2 column	806:829	Under normal-phase conditions, the two benoxacor enantiomers were completely separated on Chiralpak AD, Chiralpak IC, Lux Cellulose-1, and Lux Cellulose-3 columns and partially separated on a Lux Cellulose-2 column.					
37240233	8	76	from	enantiomers	1530:1540	arg1	types					1551:1555	three types	1545:1555	three types of horticultural soil	1545:1577	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	8	77	theme	Lux	1399:1401	arg1	column					1415:1420	the Lux Cellulose-3 column	1395:1420	the Lux Cellulose-3 column	1395:1420	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	0	78	theme	Performance	127:137	arg1	Chromatography					146:159	Reversed-Phase High Performance Liquid Chromatography	107:159	Reversed-Phase High Performance Liquid Chromatography	107:159	Enantiomeric Separation and Degradation of Benoxacor Enantiomers in Horticultural Soil by Normal-Phase and Reversed-Phase High Performance Liquid Chromatography.					
37240233	9	79	located	observed	1656:1663	arg1	methanol					1668:1675	methanol	1668:1675	methanol	1668:1675	Benoxacor enantiomers were stable, and degradation or racemization were not observed in methanol, ethanol, isopropanol, acetonitrile, hexane, or water (pH = 4.0, 7.0, and 9.0).					
37240233	9	79	located	observed	1656:1663	arg1	acetonitrile					1700:1711	acetonitrile	1700:1711	acetonitrile	1700:1711	Benoxacor enantiomers were stable, and degradation or racemization were not observed in methanol, ethanol, isopropanol, acetonitrile, hexane, or water (pH = 4.0, 7.0, and 9.0).					
37240233	9	79	located	observed	1656:1663	arg1	hexane					1714:1719	hexane	1714:1719	hexane	1714:1719	Benoxacor enantiomers were stable, and degradation or racemization were not observed in methanol, ethanol, isopropanol, acetonitrile, hexane, or water (pH = 4.0, 7.0, and 9.0).					
37240233	9	79	located	observed	1656:1663	arg2	degradation					1619:1629	degradation	1619:1629	degradation	1619:1629	Benoxacor enantiomers were stable, and degradation or racemization were not observed in methanol, ethanol, isopropanol, acetonitrile, hexane, or water (pH = 4.0, 7.0, and 9.0).					
37240233	9	79	located	observed	1656:1663	arg1	ethanol					1678:1684	ethanol	1678:1684	ethanol	1678:1684	Benoxacor enantiomers were stable, and degradation or racemization were not observed in methanol, ethanol, isopropanol, acetonitrile, hexane, or water (pH = 4.0, 7.0, and 9.0).					
37240233	9	79	located	observed	1656:1663	arg2	racemization					1634:1645	racemization	1634:1645	racemization	1634:1645	Benoxacor enantiomers were stable, and degradation or racemization were not observed in methanol, ethanol, isopropanol, acetonitrile, hexane, or water (pH = 4.0, 7.0, and 9.0).					
37240233	9	79	located	observed	1656:1663	arg1	isopropanol					1687:1697	isopropanol	1687:1697	isopropanol	1687:1697	Benoxacor enantiomers were stable, and degradation or racemization were not observed in methanol, ethanol, isopropanol, acetonitrile, hexane, or water (pH = 4.0, 7.0, and 9.0).					
37240233	9	79	located	observed	1656:1663	arg1	water					1725:1729	water	1725:1729	water (pH = 4.0, 7.0, and 9.0)	1725:1754	Benoxacor enantiomers were stable, and degradation or racemization were not observed in methanol, ethanol, isopropanol, acetonitrile, hexane, or water (pH = 4.0, 7.0, and 9.0).					
37240233	9	79	located	observed	1656:1663	arg1	pH					1732:1733	pH = 4.0, 7.0, and 9.0	1732:1753	pH = 4.0, 7.0, and 9.0	1732:1753	Benoxacor enantiomers were stable, and degradation or racemization were not observed in methanol, ethanol, isopropanol, acetonitrile, hexane, or water (pH = 4.0, 7.0, and 9.0).					
37240233	1	80	theme	reversed-phase	324:337	arg1	conditions					339:348	normal-phase and reversed-phase conditions	307:348	normal-phase and reversed-phase conditions	307:348	The separation of benoxacor enantiomers on six commercial chiral columns was investigated by high-performance liquid chromatography (HPLC) under normal-phase and reversed-phase conditions.					
37240233	3	81	theme	mobile	523:528	arg1	composition					536:546	mobile phase composition	523:546	mobile phase composition	523:546	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers were examined.					
37240233	0	82	theme	Benoxacor	43:51	arg1	Enantiomers					53:63	Benoxacor Enantiomers	43:63	Benoxacor Enantiomers	43:63	Enantiomeric Separation and Degradation of Benoxacor Enantiomers in Horticultural Soil by Normal-Phase and Reversed-Phase High Performance Liquid Chromatography.					
37240233	3	83	theme	phases	491:496	arg1	effects					458:464	The effects	454:464	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers	454:599	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers were examined.					
37240233	11	84	theme	study	1927:1931	arg1	results					1911:1917	The results	1907:1917	The results of this study	1907:1931	The results of this study will help to improve the risk assessment of enantiomer levels of benoxacor in the environment.					
37240233	7	85	theme	entropy	1193:1199	arg1	results					1206:1212	the enthalpy (ΔH) and entropy (ΔS) results	1171:1212	the enthalpy (ΔH) and entropy (ΔS) results	1171:1212	As the column temperature increased from 10 °C to 4 °C, the enthalpy (ΔH) and entropy (ΔS) results indicated that the resolution was strongly affected by the temperature and that the lowest temperature did not always produce the best resolution.					
37240233	3	86	theme	chiral	473:478	arg1	CSPs					499:502	CSPs	499:502	CSPs	499:502	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers were examined.					
37240233	3	86	theme	chiral	473:478	arg1	phases					491:496	the chiral stationary phases	469:496	the chiral stationary phases (CSPs)	469:503	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers were examined.					
37240233	11	87	theme	risk	1958:1961	arg1	assessment					1963:1972	the risk assessment	1954:1972	the risk assessment of enantiomer levels of benoxacor in the environment	1954:2025	The results of this study will help to improve the risk assessment of enantiomer levels of benoxacor in the environment.					
37240233	8	88	theme	benoxacor	1463:1471	arg1	enantiomers					1473:1483	benoxacor enantiomers	1463:1483	benoxacor enantiomers in solvents	1463:1495	An optimized separation method on the Lux Cellulose-3 column was used to investigate the stability of benoxacor enantiomers in solvents and the degradation of benoxacor enantiomers in three types of horticultural soil.					
37240233	3	89	theme	composition	536:546	arg1	effects					458:464	The effects	454:464	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers	454:599	The effects of the chiral stationary phases (CSPs), temperature, and mobile phase composition and ratio on the separation of benoxacor enantiomers were examined.					
37240233	1	90	theme	liquid	272:277	arg1	chromatography					279:292	high-performance liquid chromatography	255:292	high-performance liquid chromatography (HPLC)	255:299	The separation of benoxacor enantiomers on six commercial chiral columns was investigated by high-performance liquid chromatography (HPLC) under normal-phase and reversed-phase conditions.					
37240233	1	90	theme	liquid	272:277	arg1	HPLC					295:298	HPLC	295:298	HPLC	295:298	The separation of benoxacor enantiomers on six commercial chiral columns was investigated by high-performance liquid chromatography (HPLC) under normal-phase and reversed-phase conditions.					
37240233	10	91	with	enrichment	1878:1887	arg1	R-benoxacor					1894:1904	R-benoxacor	1894:1904	R-benoxacor	1894:1904	In three horticultural soils, the degradation rate of S-benoxacor was faster than that of R-benoxacor, resulting in soil enrichment with R-benoxacor.					
35466985	6	0	theme	pH	1031:1032	arg1	range					1034:1038	a wide pH range	1024:1038	a wide pH range from 2.0 to 8.0	1024:1054	The formed NPs were stable over a wide pH range from 2.0 to 8.0 and elevated salt concentrations from 0 to 3 mol L-1 NaCl.					
35466985	1	1	theme	-stabilized	245:255	arg1	nanoparticles					296:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles	174:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	1	theme	-stabilized	245:255	arg1	NPs					311:313	NPs	311:313	NPs	311:313	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	4	2	theme	main	832:835	arg1	interactions					768:779	hydrophobic interactions	756:779	hydrophobic interactions	756:779	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	2	theme	main	832:835	arg1	forces					796:801	electrostatic forces	782:801	electrostatic forces	782:801	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	2	theme	main	832:835	arg1	forces					845:850	the main driving forces	828:850	the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs	828:910	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	2	theme	main	832:835	arg1	bonds					817:821	hydrogen bonds	808:821	hydrogen bonds	808:821	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	8	3	theme	HepG2	1315:1319	arg1	cells					1321:1325	CD44-overexpressing HepG2 cells	1295:1325	CD44-overexpressing HepG2 cells	1295:1325	Moreover, CS coating promoted selective accumulation in CD44-overexpressing HepG2 cells, resulting in higher inhibition of tumor growth compared to free Cur and FP-NaCas NP-encapsulated Cur.					
35466985	3	4	theme	FP	533:534	arg1	cores					536:540	hydrophobic FP cores	521:540	hydrophobic FP cores	521:540	Under this condition, the microstructures of hydrophobic FP cores, amphiphilic NaCas and hydrophilic CS shells were fabricated readily by a one-pot method.					
35466985	1	5	theme	millet	265:270	arg1	nanoparticles					296:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles	174:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	5	theme	millet	265:270	arg1	NPs					311:313	NPs	311:313	NPs	311:313	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	4	6	theme	hydrogen	808:815	arg1	interactions					768:779	hydrophobic interactions	756:779	hydrophobic interactions	756:779	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	6	theme	hydrogen	808:815	arg1	forces					796:801	electrostatic forces	782:801	electrostatic forces	782:801	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	6	theme	hydrogen	808:815	arg1	forces					845:850	the main driving forces	828:850	the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs	828:910	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	6	theme	hydrogen	808:815	arg1	bonds					817:821	hydrogen bonds	808:821	hydrogen bonds	808:821	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	7	theme	FP/NaCas/CS	648:658	arg1	ratio					667:671	an optimal FP/NaCas/CS weight ratio	637:671	an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4	637:684	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	3	8	theme	NaCas	555:559	arg1	microstructures					502:516	the microstructures	498:516	the microstructures of hydrophobic FP cores, amphiphilic NaCas and hydrophilic CS shells	498:585	Under this condition, the microstructures of hydrophobic FP cores, amphiphilic NaCas and hydrophilic CS shells were fabricated readily by a one-pot method.					
35466985	1	9	theme	chondroitin	196:206	arg1	CS					217:218	CS	217:218	CS	217:218	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	9	theme	chondroitin	196:206	arg1	sulfate					208:214	curcumin (Cur)-loaded chondroitin sulfate	174:214	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	6	10	theme	salt	1069:1072	arg1	concentrations					1074:1087	salt concentrations	1069:1087	salt concentrations	1069:1087	The formed NPs were stable over a wide pH range from 2.0 to 8.0 and elevated salt concentrations from 0 to 3 mol L-1 NaCl.					
35466985	10	11	theme	preclinical	1530:1540	arg1	study					1542:1546	This preclinical study	1525:1546	This preclinical study	1525:1546	This preclinical study suggests that FP-NaCas-CS NPs could be very beneficial in terms of encapsulating hydrophobic drugs, improving the effectiveness of cancer therapies and reducing side effects on normal tissues.					
35466985	7	12	theme	encapsulation	1154:1166	arg1	efficiency					1168:1177	a higher Cur encapsulation efficiency	1141:1177	a higher Cur encapsulation efficiency of 93.4%	1141:1186	FP-NaCas-CS NPs exhibited a higher Cur encapsulation efficiency of 93.4% and re-dispersion capability after lyophilization.					
35466985	6	13	dep	mol	1101:1103	arg1	3					1099:1099	3	1099:1099	3	1099:1099	The formed NPs were stable over a wide pH range from 2.0 to 8.0 and elevated salt concentrations from 0 to 3 mol L-1 NaCl.					
35466985	3	14	theme	shells	580:585	arg1	microstructures					502:516	the microstructures	498:516	the microstructures of hydrophobic FP cores, amphiphilic NaCas and hydrophilic CS shells	498:585	Under this condition, the microstructures of hydrophobic FP cores, amphiphilic NaCas and hydrophilic CS shells were fabricated readily by a one-pot method.					
35466985	6	15	dep	NaCl	1109:1112	arg1	mol					1101:1103	mol	1101:1103	mol	1101:1103	The formed NPs were stable over a wide pH range from 2.0 to 8.0 and elevated salt concentrations from 0 to 3 mol L-1 NaCl.					
35466985	10	16	theme	FP-NaCas-CS	1562:1572	arg1	NPs					1574:1576	FP-NaCas-CS NPs	1562:1576	FP-NaCas-CS NPs	1562:1576	This preclinical study suggests that FP-NaCas-CS NPs could be very beneficial in terms of encapsulating hydrophobic drugs, improving the effectiveness of cancer therapies and reducing side effects on normal tissues.					
35466985	3	17	theme	hydrophilic	565:575	arg1	shells					580:585	hydrophilic CS shells	565:585	hydrophilic CS shells	565:585	Under this condition, the microstructures of hydrophobic FP cores, amphiphilic NaCas and hydrophilic CS shells were fabricated readily by a one-pot method.					
35466985	8	18	theme	FP-NaCas	1400:1407	arg1	Cur					1425:1427	FP-NaCas NP-encapsulated Cur	1400:1427	FP-NaCas NP-encapsulated Cur	1400:1427	Moreover, CS coating promoted selective accumulation in CD44-overexpressing HepG2 cells, resulting in higher inhibition of tumor growth compared to free Cur and FP-NaCas NP-encapsulated Cur.					
35466985	7	19	theme	higher	1143:1148	arg1	efficiency					1168:1177	a higher Cur encapsulation efficiency	1141:1177	a higher Cur encapsulation efficiency of 93.4%	1141:1186	FP-NaCas-CS NPs exhibited a higher Cur encapsulation efficiency of 93.4% and re-dispersion capability after lyophilization.					
35466985	4	20	from	nm	748:749	arg1	microstructures					719:733	globular microstructures	710:733	globular microstructures at about 145 nm	710:749	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	21	theme	3 	676:677	arg1	ratio					667:671	an optimal FP/NaCas/CS weight ratio	637:671	an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4	637:684	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	0	22	theme	physicochemical	92:106	arg1	properties					108:117	physicochemical properties	92:117	physicochemical properties	92:117	Chondroitin sulfate deposited on foxtail millet prolamin/caseinate nanoparticles to improve physicochemical properties and enhance cancer therapeutic effects.					
35466985	9	23	theme	reduced	1476:1482	arg1	cytotoxicity					1484:1495	reduced cytotoxicity	1476:1495	reduced cytotoxicity	1476:1495	As for comparison, encapsulated Cur exhibited reduced cytotoxicity on normal liver cells L-O2.					
35466985	4	24	theme	FP-NaCas-CS	896:906	arg1	NPs					908:910	stable FP-NaCas-CS NPs	889:910	stable FP-NaCas-CS NPs	889:910	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	8	25	theme	selective	1269:1277	arg1	accumulation					1279:1290	selective accumulation	1269:1290	selective accumulation in CD44-overexpressing HepG2 cells	1269:1325	Moreover, CS coating promoted selective accumulation in CD44-overexpressing HepG2 cells, resulting in higher inhibition of tumor growth compared to free Cur and FP-NaCas NP-encapsulated Cur.					
35466985	7	26	theme	FP-NaCas-CS	1115:1125	arg1	NPs					1127:1129	FP-NaCas-CS NPs	1115:1129	FP-NaCas-CS NPs	1115:1129	FP-NaCas-CS NPs exhibited a higher Cur encapsulation efficiency of 93.4% and re-dispersion capability after lyophilization.					
35466985	10	27	theme	hydrophobic	1629:1639	arg1	drugs					1641:1645	hydrophobic drugs	1629:1645	hydrophobic drugs	1629:1645	This preclinical study suggests that FP-NaCas-CS NPs could be very beneficial in terms of encapsulating hydrophobic drugs, improving the effectiveness of cancer therapies and reducing side effects on normal tissues.					
35466985	0	28	theme	cancer	131:136	arg1	effects					150:156	cancer therapeutic effects	131:156	cancer therapeutic effects	131:156	Chondroitin sulfate deposited on foxtail millet prolamin/caseinate nanoparticles to improve physicochemical properties and enhance cancer therapeutic effects.					
35466985	9	29	theme	liver	1507:1511	arg1	cells					1513:1517	normal liver cells	1500:1517	normal liver cells L-O2	1500:1522	As for comparison, encapsulated Cur exhibited reduced cytotoxicity on normal liver cells L-O2.					
35466985	4	30	theme	FP-NaCas-CS	687:697	arg1	NPs					699:701	FP-NaCas-CS NPs	687:701	FP-NaCas-CS NPs	687:701	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	0	31	theme	Chondroitin	0:10	arg1	sulfate					12:18	Chondroitin sulfate	0:18	Chondroitin sulfate deposited on foxtail millet prolamin/caseinate	0:65	Chondroitin sulfate deposited on foxtail millet prolamin/caseinate nanoparticles to improve physicochemical properties and enhance cancer therapeutic effects.					
35466985	1	32	theme	prolamin	272:279	arg1	nanoparticles					296:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles	174:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	32	theme	prolamin	272:279	arg1	NPs					311:313	NPs	311:313	NPs	311:313	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	2	33	theme	antisolvent	397:407	arg1	precipitation					409:421	liquid antisolvent precipitation	390:421	liquid antisolvent precipitation under neutral and alkaline conditions (pH 7.0-11.0)	390:473	FP is capable of self-assembly via liquid antisolvent precipitation under neutral and alkaline conditions (pH 7.0-11.0).					
35466985	1	34	theme	FP	282:283	arg1	nanoparticles					296:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles	174:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	34	theme	FP	282:283	arg1	NPs					311:313	NPs	311:313	NPs	311:313	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	6	35	theme	formed	996:1001	arg1	stable					1012:1017	stable	1012:1017	stable	1012:1017	The formed NPs were stable over a wide pH range from 2.0 to 8.0 and elevated salt concentrations from 0 to 3 mol L-1 NaCl.					
35466985	6	35	theme	formed	996:1001	arg1	NPs					1003:1005	The formed NPs	992:1005	The formed NPs	992:1005	The formed NPs were stable over a wide pH range from 2.0 to 8.0 and elevated salt concentrations from 0 to 3 mol L-1 NaCl.					
35466985	0	36	theme	foxtail	33:39	arg1	prolamin/caseinate					48:65	foxtail millet prolamin/caseinate	33:65	foxtail millet prolamin/caseinate	33:65	Chondroitin sulfate deposited on foxtail millet prolamin/caseinate nanoparticles to improve physicochemical properties and enhance cancer therapeutic effects.					
35466985	1	37	theme	composite	286:294	arg1	nanoparticles					296:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles	174:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	37	theme	composite	286:294	arg1	NPs					311:313	NPs	311:313	NPs	311:313	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	5	38	theme	ionic	966:970	arg1	strength					972:979	the ionic strength	962:979	the ionic strength stability	962:989	CS coating enhanced the pH stability but reduced the ionic strength stability.					
35466985	10	39	from	beneficial	1592:1601	arg1	terms					1606:1610	terms	1606:1610	terms of encapsulating hydrophobic drugs, improving the effectiveness of cancer therapies and reducing side effects on normal tissues	1606:1738	This preclinical study suggests that FP-NaCas-CS NPs could be very beneficial in terms of encapsulating hydrophobic drugs, improving the effectiveness of cancer therapies and reducing side effects on normal tissues.					
35466985	3	40	theme	one-pot	616:622	arg1	method					624:629	a one-pot method	614:629	a one-pot method	614:629	Under this condition, the microstructures of hydrophobic FP cores, amphiphilic NaCas and hydrophilic CS shells were fabricated readily by a one-pot method.					
35466985	4	41	theme	electrostatic	782:794	arg1	interactions					768:779	hydrophobic interactions	756:779	hydrophobic interactions	756:779	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	41	theme	electrostatic	782:794	arg1	forces					796:801	electrostatic forces	782:801	electrostatic forces	782:801	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	41	theme	electrostatic	782:794	arg1	forces					845:850	the main driving forces	828:850	the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs	828:910	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	41	theme	electrostatic	782:794	arg1	bonds					817:821	hydrogen bonds	808:821	hydrogen bonds	808:821	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	10	42	from	terms	1606:1610	arg1	beneficial					1592:1601	beneficial	1592:1601	beneficial	1592:1601	This preclinical study suggests that FP-NaCas-CS NPs could be very beneficial in terms of encapsulating hydrophobic drugs, improving the effectiveness of cancer therapies and reducing side effects on normal tissues.					
35466985	7	43	theme	re-dispersion	1192:1204	arg1	capability					1206:1215	re-dispersion capability	1192:1215	re-dispersion capability	1192:1215	FP-NaCas-CS NPs exhibited a higher Cur encapsulation efficiency of 93.4% and re-dispersion capability after lyophilization.					
35466985	8	44	theme	tumor	1362:1366	arg1	growth					1368:1373	tumor growth	1362:1373	tumor growth	1362:1373	Moreover, CS coating promoted selective accumulation in CD44-overexpressing HepG2 cells, resulting in higher inhibition of tumor growth compared to free Cur and FP-NaCas NP-encapsulated Cur.					
35466985	6	45	from	8.0	1052:1054	arg1	range					1034:1038	a wide pH range	1024:1038	a wide pH range from 2.0 to 8.0	1024:1054	The formed NPs were stable over a wide pH range from 2.0 to 8.0 and elevated salt concentrations from 0 to 3 mol L-1 NaCl.					
35466985	8	46	from	accumulation	1279:1290	arg1	cells					1321:1325	CD44-overexpressing HepG2 cells	1295:1325	CD44-overexpressing HepG2 cells	1295:1325	Moreover, CS coating promoted selective accumulation in CD44-overexpressing HepG2 cells, resulting in higher inhibition of tumor growth compared to free Cur and FP-NaCas NP-encapsulated Cur.					
35466985	1	47	theme	-sodium	220:226	arg1	nanoparticles					296:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles	174:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	47	theme	-sodium	220:226	arg1	NPs					311:313	NPs	311:313	NPs	311:313	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	7	48	theme	%	1186:1186	arg1	efficiency					1168:1177	a higher Cur encapsulation efficiency	1141:1177	a higher Cur encapsulation efficiency of 93.4%	1141:1186	FP-NaCas-CS NPs exhibited a higher Cur encapsulation efficiency of 93.4% and re-dispersion capability after lyophilization.					
35466985	7	48	theme	%	1186:1186	arg1	capability					1206:1215	re-dispersion capability	1192:1215	re-dispersion capability	1192:1215	FP-NaCas-CS NPs exhibited a higher Cur encapsulation efficiency of 93.4% and re-dispersion capability after lyophilization.					
35466985	2	49	theme	self-assembly	372:384	arg1	capable					361:367	capable	361:367	capable	361:367	FP is capable of self-assembly via liquid antisolvent precipitation under neutral and alkaline conditions (pH 7.0-11.0).					
35466985	10	50	theme	cancer	1679:1684	arg1	therapies					1686:1694	cancer therapies	1679:1694	cancer therapies	1679:1694	This preclinical study suggests that FP-NaCas-CS NPs could be very beneficial in terms of encapsulating hydrophobic drugs, improving the effectiveness of cancer therapies and reducing side effects on normal tissues.					
35466985	6	51	theme	wide	1026:1029	arg1	range					1034:1038	a wide pH range	1024:1038	a wide pH range from 2.0 to 8.0	1024:1054	The formed NPs were stable over a wide pH range from 2.0 to 8.0 and elevated salt concentrations from 0 to 3 mol L-1 NaCl.					
35466985	3	52	theme	hydrophobic	521:531	arg1	cores					536:540	hydrophobic FP cores	521:540	hydrophobic FP cores	521:540	Under this condition, the microstructures of hydrophobic FP cores, amphiphilic NaCas and hydrophilic CS shells were fabricated readily by a one-pot method.					
35466985	1	53	theme	foxtail	257:263	arg1	nanoparticles					296:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles	174:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	53	theme	foxtail	257:263	arg1	NPs					311:313	NPs	311:313	NPs	311:313	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	10	54	theme	side	1709:1712	arg1	effects					1714:1720	side effects	1709:1720	side effects on normal tissues	1709:1738	This preclinical study suggests that FP-NaCas-CS NPs could be very beneficial in terms of encapsulating hydrophobic drugs, improving the effectiveness of cancer therapies and reducing side effects on normal tissues.					
35466985	1	55	theme	Cur	184:186	arg1	CS					217:218	CS	217:218	CS	217:218	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	55	theme	Cur	184:186	arg1	sulfate					208:214	curcumin (Cur)-loaded chondroitin sulfate	174:214	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	3	56	theme	amphiphilic	543:553	arg1	NaCas					555:559	amphiphilic NaCas	543:559	amphiphilic NaCas	543:559	Under this condition, the microstructures of hydrophobic FP cores, amphiphilic NaCas and hydrophilic CS shells were fabricated readily by a one-pot method.					
35466985	1	57	theme	-loaded	188:194	arg1	CS					217:218	CS	217:218	CS	217:218	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	57	theme	-loaded	188:194	arg1	sulfate					208:214	curcumin (Cur)-loaded chondroitin sulfate	174:214	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	4	58	theme	optimal	640:646	arg1	ratio					667:671	an optimal FP/NaCas/CS weight ratio	637:671	an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4	637:684	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	3	59	theme	cores	536:540	arg1	microstructures					502:516	the microstructures	498:516	the microstructures of hydrophobic FP cores, amphiphilic NaCas and hydrophilic CS shells	498:585	Under this condition, the microstructures of hydrophobic FP cores, amphiphilic NaCas and hydrophilic CS shells were fabricated readily by a one-pot method.					
35466985	1	60	theme	sulfate	208:214	arg1	nanoparticles					296:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles	174:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	60	theme	sulfate	208:214	arg1	NPs					311:313	NPs	311:313	NPs	311:313	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	8	61	theme	free	1387:1390	arg1	Cur					1392:1394	free Cur	1387:1394	free Cur	1387:1394	Moreover, CS coating promoted selective accumulation in CD44-overexpressing HepG2 cells, resulting in higher inhibition of tumor growth compared to free Cur and FP-NaCas NP-encapsulated Cur.					
35466985	3	62	theme	CS	577:578	arg1	shells					580:585	hydrophilic CS shells	565:585	hydrophilic CS shells	565:585	Under this condition, the microstructures of hydrophobic FP cores, amphiphilic NaCas and hydrophilic CS shells were fabricated readily by a one-pot method.					
35466985	6	63	dep	3	1099:1099	arg1	to					1096:1097	to	1096:1097	to	1096:1097	The formed NPs were stable over a wide pH range from 2.0 to 8.0 and elevated salt concentrations from 0 to 3 mol L-1 NaCl.					
35466985	4	64	theme	driving	837:843	arg1	interactions					768:779	hydrophobic interactions	756:779	hydrophobic interactions	756:779	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	64	theme	driving	837:843	arg1	forces					796:801	electrostatic forces	782:801	electrostatic forces	782:801	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	64	theme	driving	837:843	arg1	forces					845:850	the main driving forces	828:850	the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs	828:910	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	64	theme	driving	837:843	arg1	bonds					817:821	hydrogen bonds	808:821	hydrogen bonds	808:821	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	8	65	theme	NP-encapsulated	1409:1423	arg1	Cur					1425:1427	FP-NaCas NP-encapsulated Cur	1400:1427	FP-NaCas NP-encapsulated Cur	1400:1427	Moreover, CS coating promoted selective accumulation in CD44-overexpressing HepG2 cells, resulting in higher inhibition of tumor growth compared to free Cur and FP-NaCas NP-encapsulated Cur.					
35466985	9	66	theme	encapsulated	1449:1460	arg1	Cur					1462:1464	encapsulated Cur	1449:1464	encapsulated Cur	1449:1464	As for comparison, encapsulated Cur exhibited reduced cytotoxicity on normal liver cells L-O2.					
35466985	10	67	from	effects	1714:1720	arg1	tissues					1732:1738	normal tissues	1725:1738	normal tissues	1725:1738	This preclinical study suggests that FP-NaCas-CS NPs could be very beneficial in terms of encapsulating hydrophobic drugs, improving the effectiveness of cancer therapies and reducing side effects on normal tissues.					
35466985	6	68	theme	L-1	1105:1107	arg1	NaCl					1109:1112	0 to 3 mol L-1 NaCl	1094:1112	0 to 3 mol L-1 NaCl	1094:1112	The formed NPs were stable over a wide pH range from 2.0 to 8.0 and elevated salt concentrations from 0 to 3 mol L-1 NaCl.					
35466985	4	69	theme	weight	660:665	arg1	ratio					667:671	an optimal FP/NaCas/CS weight ratio	637:671	an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4	637:684	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	1	70	theme	curcumin	174:181	arg1	CS					217:218	CS	217:218	CS	217:218	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	70	theme	curcumin	174:181	arg1	sulfate					208:214	curcumin (Cur)-loaded chondroitin sulfate	174:214	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	8	71	theme	CS	1249:1250	arg1	coating					1252:1258	CS coating	1249:1258	CS coating	1249:1258	Moreover, CS coating promoted selective accumulation in CD44-overexpressing HepG2 cells, resulting in higher inhibition of tumor growth compared to free Cur and FP-NaCas NP-encapsulated Cur.					
35466985	5	72	theme	CS	913:914	arg1	coating					916:922	CS coating	913:922	CS coating	913:922	CS coating enhanced the pH stability but reduced the ionic strength stability.					
35466985	10	73	theme	normal	1725:1730	arg1	tissues					1732:1738	normal tissues	1725:1738	normal tissues	1725:1738	This preclinical study suggests that FP-NaCas-CS NPs could be very beneficial in terms of encapsulating hydrophobic drugs, improving the effectiveness of cancer therapies and reducing side effects on normal tissues.					
35466985	4	74	theme	stable	889:894	arg1	NPs					908:910	stable FP-NaCas-CS NPs	889:910	stable FP-NaCas-CS NPs	889:910	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	75	theme	globular	710:717	arg1	microstructures					719:733	globular microstructures	710:733	globular microstructures at about 145 nm	710:749	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	0	76	theme	therapeutic	138:148	arg1	effects					150:156	cancer therapeutic effects	131:156	cancer therapeutic effects	131:156	Chondroitin sulfate deposited on foxtail millet prolamin/caseinate nanoparticles to improve physicochemical properties and enhance cancer therapeutic effects.					
35466985	1	77	theme	one-pot	338:344	arg1	process					346:352	a one-pot process	336:352	a one-pot process	336:352	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	4	78	dep	ratio	667:671	arg1	 2 					679:681	 2 	679:681	 2 	679:681	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	78	dep	ratio	667:671	arg1	 4					683:684	 4	683:684	 4	683:684	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	79	theme	NPs	908:910	arg1	maintenance					874:884	maintenance	874:884	maintenance	874:884	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	79	theme	NPs	908:910	arg1	formation					860:868	formation	860:868	formation	860:868	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	9	80	theme	cells	1513:1517	arg1	L-O2					1519:1522	normal liver cells L-O2	1500:1522	normal liver cells L-O2	1500:1522	As for comparison, encapsulated Cur exhibited reduced cytotoxicity on normal liver cells L-O2.					
35466985	2	81	theme	neutral	429:435	arg1	conditions					450:459	neutral and alkaline conditions	429:459	conditions	450:459	FP is capable of self-assembly via liquid antisolvent precipitation under neutral and alkaline conditions (pH 7.0-11.0).					
35466985	2	81	theme	neutral	429:435	arg1	pH					462:463	pH 7.0-11.0	462:472	pH 7.0-11.0	462:472	FP is capable of self-assembly via liquid antisolvent precipitation under neutral and alkaline conditions (pH 7.0-11.0).					
35466985	8	82	theme	CD44-overexpressing	1295:1313	arg1	cells					1321:1325	CD44-overexpressing HepG2 cells	1295:1325	CD44-overexpressing HepG2 cells	1295:1325	Moreover, CS coating promoted selective accumulation in CD44-overexpressing HepG2 cells, resulting in higher inhibition of tumor growth compared to free Cur and FP-NaCas NP-encapsulated Cur.					
35466985	9	83	theme	normal	1500:1505	arg1	cells					1513:1517	normal liver cells	1500:1517	normal liver cells L-O2	1500:1522	As for comparison, encapsulated Cur exhibited reduced cytotoxicity on normal liver cells L-O2.					
35466985	2	84	theme	liquid	390:395	arg1	precipitation					409:421	liquid antisolvent precipitation	390:421	liquid antisolvent precipitation under neutral and alkaline conditions (pH 7.0-11.0)	390:473	FP is capable of self-assembly via liquid antisolvent precipitation under neutral and alkaline conditions (pH 7.0-11.0).					
35466985	0	85	theme	millet	41:46	arg1	prolamin/caseinate					48:65	foxtail millet prolamin/caseinate	33:65	foxtail millet prolamin/caseinate	33:65	Chondroitin sulfate deposited on foxtail millet prolamin/caseinate nanoparticles to improve physicochemical properties and enhance cancer therapeutic effects.					
35466985	4	86	theme	hydrophobic	756:766	arg1	interactions					768:779	hydrophobic interactions	756:779	hydrophobic interactions	756:779	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	86	theme	hydrophobic	756:766	arg1	forces					796:801	electrostatic forces	782:801	electrostatic forces	782:801	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	86	theme	hydrophobic	756:766	arg1	forces					845:850	the main driving forces	828:850	the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs	828:910	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	86	theme	hydrophobic	756:766	arg1	bonds					817:821	hydrogen bonds	808:821	hydrogen bonds	808:821	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	4	87	dep	formation	860:868	arg1	the					856:858	the	856:858	the	856:858	With an optimal FP/NaCas/CS weight ratio of 3 : 2 : 4, FP-NaCas-CS NPs shared globular microstructures at about 145 nm, and hydrophobic interactions, electrostatic forces, and hydrogen bonds were the main driving forces for the formation and maintenance of stable FP-NaCas-CS NPs.					
35466985	5	88	theme	strength	972:979	arg1	stability					981:989	the ionic strength stability	962:989	the ionic strength stability	962:989	CS coating enhanced the pH stability but reduced the ionic strength stability.					
35466985	2	89	theme	alkaline	441:448	arg1	conditions					450:459	neutral and alkaline conditions	429:459	conditions	450:459	FP is capable of self-assembly via liquid antisolvent precipitation under neutral and alkaline conditions (pH 7.0-11.0).					
35466985	2	89	theme	alkaline	441:448	arg1	pH					462:463	pH 7.0-11.0	462:472	pH 7.0-11.0	462:472	FP is capable of self-assembly via liquid antisolvent precipitation under neutral and alkaline conditions (pH 7.0-11.0).					
35466985	8	90	theme	higher	1341:1346	arg1	inhibition					1348:1357	higher inhibition	1341:1357	higher inhibition of tumor growth	1341:1373	Moreover, CS coating promoted selective accumulation in CD44-overexpressing HepG2 cells, resulting in higher inhibition of tumor growth compared to free Cur and FP-NaCas NP-encapsulated Cur.					
35466985	6	91	dep	8.0	1052:1054	arg1	to					1049:1050	to	1049:1050	to	1049:1050	The formed NPs were stable over a wide pH range from 2.0 to 8.0 and elevated salt concentrations from 0 to 3 mol L-1 NaCl.					
35466985	5	92	theme	pH	937:938	arg1	stability					940:948	the pH stability	933:948	the pH stability	933:948	CS coating enhanced the pH stability but reduced the ionic strength stability.					
35466985	1	93	theme	caseinate	228:236	arg1	nanoparticles					296:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles	174:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	93	theme	caseinate	228:236	arg1	NPs					311:313	NPs	311:313	NPs	311:313	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	8	94	theme	growth	1368:1373	arg1	inhibition					1348:1357	higher inhibition	1341:1357	higher inhibition of tumor growth	1341:1373	Moreover, CS coating promoted selective accumulation in CD44-overexpressing HepG2 cells, resulting in higher inhibition of tumor growth compared to free Cur and FP-NaCas NP-encapsulated Cur.					
35466985	10	95	theme	therapies	1686:1694	arg1	effectiveness					1662:1674	the effectiveness	1658:1674	the effectiveness of cancer therapies	1658:1694	This preclinical study suggests that FP-NaCas-CS NPs could be very beneficial in terms of encapsulating hydrophobic drugs, improving the effectiveness of cancer therapies and reducing side effects on normal tissues.					
35466985	7	96	theme	Cur	1150:1152	arg1	efficiency					1168:1177	a higher Cur encapsulation efficiency	1141:1177	a higher Cur encapsulation efficiency of 93.4%	1141:1186	FP-NaCas-CS NPs exhibited a higher Cur encapsulation efficiency of 93.4% and re-dispersion capability after lyophilization.					
35466985	1	97	theme	NaCas	239:243	arg1	nanoparticles					296:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles	174:308	curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs)	174:314	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35466985	1	97	theme	NaCas	239:243	arg1	NPs					311:313	NPs	311:313	NPs	311:313	In this study, curcumin (Cur)-loaded chondroitin sulfate (CS)-sodium caseinate (NaCas)-stabilized foxtail millet prolamin (FP) composite nanoparticles (NPs) were fabricated via a one-pot process.					
35469076	7	0	theme	liquid	1352:1357	arg1	chromatography					1359:1372	high-performance liquid chromatography	1335:1372	a high-performance liquid chromatography method with relative error of 5.3%	1333:1407	Sulfamethoxazole was actually found in crucian sample with 12.4 μg/kg, and the result was confirmed by a high-performance liquid chromatography method with relative error of 5.3%.					
35469076	3	1	theme	The grown	589:597	arg1	AgNPs					599:603	The grown AgNPs	589:603	The grown AgNPs	589:603	The grown AgNPs can provide abundant hot spots and plasmonic areas to amplify the Raman signals of target molecules effectively.					
35469076	0	2	from	food	184:187	arg1	determination					132:144	enrichment and surface-enhanced Raman scattering determination	83:144	enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food	83:187	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	7	3	located	found	1260:1264	arg2	Sulfamethoxazole					1230:1245	Sulfamethoxazole	1230:1245	Sulfamethoxazole	1230:1245	Sulfamethoxazole was actually found in crucian sample with 12.4 μg/kg, and the result was confirmed by a high-performance liquid chromatography method with relative error of 5.3%.					
35469076	7	3	located	found	1260:1264	arg1	sample					1277:1282	crucian sample	1269:1282	crucian sample with 12.4 μg/kg	1269:1298	Sulfamethoxazole was actually found in crucian sample with 12.4 μg/kg, and the result was confirmed by a high-performance liquid chromatography method with relative error of 5.3%.					
35469076	9	4	theme	Sep/CTs/AgNPs	1527:1539	arg1	substrate					1550:1558	Sep/CTs/AgNPs membrane substrate	1527:1558	Sep/CTs/AgNPs membrane substrate	1527:1558	The SERS method based on Sep/CTs/AgNPs membrane substrate provided an integrated strategy for rapid and accurate SERS analysis in food safety issues.					
35469076	4	5	theme	good	751:754	arg1	stability					756:764	good stability	751:764	good stability	751:764	The membrane substrate exhibited good stability with relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively, by detecting sulfamethoxazole.					
35469076	3	6	theme	abundant	617:624	arg1	spots					630:634	abundant hot spots	617:634	abundant hot spots	617:634	The grown AgNPs can provide abundant hot spots and plasmonic areas to amplify the Raman signals of target molecules effectively.					
35469076	1	7	theme	nanoparticles	218:230	arg1	substrate					257:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	1	7	theme	nanoparticles	218:230	arg1	determination					368:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination	276:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one	276:411	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	9	8	from	analysis	1620:1627	arg1	issues					1644:1649	food safety issues	1632:1649	food safety issues	1632:1649	The SERS method based on Sep/CTs/AgNPs membrane substrate provided an integrated strategy for rapid and accurate SERS analysis in food safety issues.					
35469076	7	9	with	sample	1277:1282	arg1	12.4 μg/kg					1289:1298	12.4 μg/kg	1289:1298	12.4 μg/kg	1289:1298	Sulfamethoxazole was actually found in crucian sample with 12.4 μg/kg, and the result was confirmed by a high-performance liquid chromatography method with relative error of 5.3%.					
35469076	1	10	theme	Sep/CTs/AgNPs	233:245	arg1	substrate					257:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	1	10	theme	Sep/CTs/AgNPs	233:245	arg1	determination					368:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination	276:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one	276:411	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	9	11	theme	membrane	1541:1548	arg1	substrate					1550:1558	Sep/CTs/AgNPs membrane substrate	1527:1558	Sep/CTs/AgNPs membrane substrate	1527:1558	The SERS method based on Sep/CTs/AgNPs membrane substrate provided an integrated strategy for rapid and accurate SERS analysis in food safety issues.					
35469076	2	12	contain	possessed	451:459	arg2	ability					465:471	the ability	461:471	the ability of rapid separation and enrichment to simplify the process for pretreatment and improve the efficiency of analysis	461:586	The Sep/CTs/AgNPs membrane substrate possessed the ability of rapid separation and enrichment to simplify the process for pretreatment and improve the efficiency of analysis.					
35469076	2	12	contain	possessed	451:459	arg1	substrate					441:449	The Sep/CTs/AgNPs membrane substrate	414:449	The Sep/CTs/AgNPs membrane substrate	414:449	The Sep/CTs/AgNPs membrane substrate possessed the ability of rapid separation and enrichment to simplify the process for pretreatment and improve the efficiency of analysis.					
35469076	3	13	theme	plasmonic	640:648	arg1	areas					650:654	plasmonic areas	640:654	plasmonic areas	640:654	The grown AgNPs can provide abundant hot spots and plasmonic areas to amplify the Raman signals of target molecules effectively.					
35469076	5	14	theme	SERS	922:925	arg1	method					927:932	The SERS method	918:932	The SERS method based on Sep/CTs/AgNPs membrane substrate	918:974	The SERS method based on Sep/CTs/AgNPs membrane substrate was used for the determination of sulfamethoxazole with a linear range of 0.05-2.0 mg/L, and the limit of detection was 0.020 mg/L.					
35469076	9	15	theme	accurate	1606:1613	arg1	analysis					1620:1627	rapid and accurate SERS analysis	1596:1627	rapid and accurate SERS analysis in food safety issues	1596:1649	The SERS method based on Sep/CTs/AgNPs membrane substrate provided an integrated strategy for rapid and accurate SERS analysis in food safety issues.					
35469076	0	16	theme	sulfamethoxazole	149:164	arg1	determination					132:144	enrichment and surface-enhanced Raman scattering determination	83:144	enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food	83:187	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	9	17	theme	rapid	1596:1600	arg1	analysis					1620:1627	rapid and accurate SERS analysis	1596:1627	rapid and accurate SERS analysis in food safety issues	1596:1649	The SERS method based on Sep/CTs/AgNPs membrane substrate provided an integrated strategy for rapid and accurate SERS analysis in food safety issues.					
35469076	4	18	theme	%	806:806	arg1	deviations					789:798	relative standard deviations	771:798	relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively	771:884	The membrane substrate exhibited good stability with relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively, by detecting sulfamethoxazole.					
35469076	0	19	theme	animal-derived	169:182	arg1	food					184:187	animal-derived food	169:187	animal-derived food	169:187	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	5	20	theme	sulfamethoxazole	1010:1025	arg1	determination					993:1005	the determination	989:1005	the determination of sulfamethoxazole	989:1025	The SERS method based on Sep/CTs/AgNPs membrane substrate was used for the determination of sulfamethoxazole with a linear range of 0.05-2.0 mg/L, and the limit of detection was 0.020 mg/L.					
35469076	8	21	theme	whole	1414:1418	arg1	process					1420:1426	The whole process	1410:1426	The whole process of analysis	1410:1438	The whole process of analysis can be finished within 25 min with recoveries of 89.3-102.2%.					
35469076	8	22	with	25 min	1463:1468	arg1	recoveries					1475:1484	recoveries	1475:1484	recoveries of 89.3-102.2%	1475:1499	The whole process of analysis can be finished within 25 min with recoveries of 89.3-102.2%.					
35469076	6	23	theme	established	1112:1122	arg1	method					1129:1134	The established SERS method	1108:1134	The established SERS method	1108:1134	The established SERS method was finally applied to the quantification of sulfamethoxazole in animal-derived food samples.					
35469076	2	24	theme	analysis	579:586	arg1	efficiency					565:574	the efficiency	561:574	the efficiency of analysis	561:586	The Sep/CTs/AgNPs membrane substrate possessed the ability of rapid separation and enrichment to simplify the process for pretreatment and improve the efficiency of analysis.					
35469076	4	25	theme	standard	780:787	arg1	deviations					789:798	relative standard deviations	771:798	relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively	771:884	The membrane substrate exhibited good stability with relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively, by detecting sulfamethoxazole.					
35469076	7	26	theme	high-performance	1335:1350	arg1	chromatography					1359:1372	high-performance liquid chromatography	1335:1372	a high-performance liquid chromatography method with relative error of 5.3%	1333:1407	Sulfamethoxazole was actually found in crucian sample with 12.4 μg/kg, and the result was confirmed by a high-performance liquid chromatography method with relative error of 5.3%.					
35469076	3	27	theme	target	688:693	arg1	molecules					695:703	target molecules	688:703	target molecules	688:703	The grown AgNPs can provide abundant hot spots and plasmonic areas to amplify the Raman signals of target molecules effectively.					
35469076	9	28	theme	food	1632:1635	arg1	issues					1644:1649	food safety issues	1632:1649	food safety issues	1632:1649	The SERS method based on Sep/CTs/AgNPs membrane substrate provided an integrated strategy for rapid and accurate SERS analysis in food safety issues.					
35469076	1	29	theme	all-in-one	402:411	arg1	sulfamethoxazole					385:400	sulfamethoxazole all-in-one	385:411	sulfamethoxazole all-in-one	385:411	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	4	30	theme	different	835:843	arg1	batches					845:851	different batches	835:851	different batches	835:851	The membrane substrate exhibited good stability with relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively, by detecting sulfamethoxazole.					
35469076	7	31	theme	chromatography	1359:1372	arg1	method					1374:1379	a high-performance liquid chromatography method	1333:1379	a high-performance liquid chromatography method with relative error of 5.3%	1333:1407	Sulfamethoxazole was actually found in crucian sample with 12.4 μg/kg, and the result was confirmed by a high-performance liquid chromatography method with relative error of 5.3%.					
35469076	4	32	theme	membrane	853:860	arg1	substrate					862:870	membrane substrate	853:870	membrane substrate	853:870	The membrane substrate exhibited good stability with relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively, by detecting sulfamethoxazole.					
35469076	0	33	theme	Raman	115:119	arg1	scattering					121:130	surface-enhanced Raman scattering	98:130	surface-enhanced Raman scattering	98:130	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	0	34	theme	Flexible	0:7	arg1	composite					18:26	Flexible membrane composite	0:26	Flexible membrane composite	0:26	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	2	35	theme	enrichment	497:506	arg1	ability					465:471	the ability	461:471	the ability of rapid separation and enrichment to simplify the process for pretreatment and improve the efficiency of analysis	461:586	The Sep/CTs/AgNPs membrane substrate possessed the ability of rapid separation and enrichment to simplify the process for pretreatment and improve the efficiency of analysis.					
35469076	1	36	theme	Raman	344:348	arg1	scattering					350:359	surface-enhanced Raman scattering	327:359	surface-enhanced Raman scattering	327:359	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	5	37	theme	0.05-2.0 mg/L	1050:1062	arg1	range					1041:1045	a linear range	1032:1045	a linear range of 0.05-2.0 mg/L	1032:1062	The SERS method based on Sep/CTs/AgNPs membrane substrate was used for the determination of sulfamethoxazole with a linear range of 0.05-2.0 mg/L, and the limit of detection was 0.020 mg/L.					
35469076	2	38	theme	separation	482:491	arg1	ability					465:471	the ability	461:471	the ability of rapid separation and enrichment to simplify the process for pretreatment and improve the efficiency of analysis	461:586	The Sep/CTs/AgNPs membrane substrate possessed the ability of rapid separation and enrichment to simplify the process for pretreatment and improve the efficiency of analysis.					
35469076	7	39	theme	crucian	1269:1275	arg1	sample					1277:1282	crucian sample	1269:1282	crucian sample with 12.4 μg/kg	1269:1298	Sulfamethoxazole was actually found in crucian sample with 12.4 μg/kg, and the result was confirmed by a high-performance liquid chromatography method with relative error of 5.3%.					
35469076	6	40	from	samples	1221:1227	arg1	quantification					1163:1176	the quantification	1159:1176	the quantification of sulfamethoxazole in animal-derived food samples	1159:1227	The established SERS method was finally applied to the quantification of sulfamethoxazole in animal-derived food samples.					
35469076	5	41	theme	detection	1082:1090	arg1	0.020 mg/L					1096:1105	0.020 mg/L	1096:1105	0.020 mg/L	1096:1105	The SERS method based on Sep/CTs/AgNPs membrane substrate was used for the determination of sulfamethoxazole with a linear range of 0.05-2.0 mg/L, and the limit of detection was 0.020 mg/L.					
35469076	5	41	theme	detection	1082:1090	arg1	limit					1073:1077	the limit	1069:1077	the limit of detection	1069:1090	The SERS method based on Sep/CTs/AgNPs membrane substrate was used for the determination of sulfamethoxazole with a linear range of 0.05-2.0 mg/L, and the limit of detection was 0.020 mg/L.					
35469076	0	42	from	sulfamethoxazole	149:164	arg1	food					184:187	animal-derived food	169:187	animal-derived food	169:187	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	6	43	link	animal-derived	1201:1214	arg1	samples					1221:1227	animal-derived food samples	1201:1227	animal-derived food samples	1201:1227	The established SERS method was finally applied to the quantification of sulfamethoxazole in animal-derived food samples.					
35469076	6	44	from	sulfamethoxazole	1181:1196	arg1	samples					1221:1227	animal-derived food samples	1201:1227	animal-derived food samples	1201:1227	The established SERS method was finally applied to the quantification of sulfamethoxazole in animal-derived food samples.					
35469076	5	45	theme	Sep/CTs/AgNPs	943:955	arg1	substrate					966:974	Sep/CTs/AgNPs membrane substrate	943:974	Sep/CTs/AgNPs membrane substrate	943:974	The SERS method based on Sep/CTs/AgNPs membrane substrate was used for the determination of sulfamethoxazole with a linear range of 0.05-2.0 mg/L, and the limit of detection was 0.020 mg/L.					
35469076	6	46	theme	animal-derived	1201:1214	arg1	samples					1221:1227	animal-derived food samples	1201:1227	animal-derived food samples	1201:1227	The established SERS method was finally applied to the quantification of sulfamethoxazole in animal-derived food samples.					
35469076	0	47	from	determination	132:144	arg1	food					184:187	animal-derived food	169:187	animal-derived food	169:187	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	2	48	theme	Sep/CTs/AgNPs	418:430	arg1	substrate					441:449	The Sep/CTs/AgNPs membrane substrate	414:449	The Sep/CTs/AgNPs membrane substrate	414:449	The Sep/CTs/AgNPs membrane substrate possessed the ability of rapid separation and enrichment to simplify the process for pretreatment and improve the efficiency of analysis.					
35469076	1	49	theme	separation	299:308	arg1	substrate					257:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	1	49	theme	separation	299:308	arg1	determination					368:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination	276:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one	276:411	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	6	50	theme	sulfamethoxazole	1181:1196	arg1	quantification					1163:1176	the quantification	1159:1176	the quantification of sulfamethoxazole in animal-derived food samples	1159:1227	The established SERS method was finally applied to the quantification of sulfamethoxazole in animal-derived food samples.					
35469076	7	51	with	method	1374:1379	arg1	error					1395:1399	relative error	1386:1399	relative error of 5.3%	1386:1407	Sulfamethoxazole was actually found in crucian sample with 12.4 μg/kg, and the result was confirmed by a high-performance liquid chromatography method with relative error of 5.3%.					
35469076	3	52	theme	hot	626:628	arg1	spots					630:634	abundant hot spots	617:634	abundant hot spots	617:634	The grown AgNPs can provide abundant hot spots and plasmonic areas to amplify the Raman signals of target molecules effectively.					
35469076	1	53	theme	enrichment	311:320	arg1	substrate					257:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	1	53	theme	enrichment	311:320	arg1	determination					368:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination	276:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one	276:411	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	6	54	from	quantification	1163:1176	arg1	samples					1221:1227	animal-derived food samples	1201:1227	animal-derived food samples	1201:1227	The established SERS method was finally applied to the quantification of sulfamethoxazole in animal-derived food samples.					
35469076	1	55	theme	sepiolite/chitosan/silver	192:216	arg1	substrate					257:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	1	55	theme	sepiolite/chitosan/silver	192:216	arg1	determination					368:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination	276:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one	276:411	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	5	56	theme	membrane	957:964	arg1	substrate					966:974	Sep/CTs/AgNPs membrane substrate	943:974	Sep/CTs/AgNPs membrane substrate	943:974	The SERS method based on Sep/CTs/AgNPs membrane substrate was used for the determination of sulfamethoxazole with a linear range of 0.05-2.0 mg/L, and the limit of detection was 0.020 mg/L.					
35469076	9	57	theme	integrated	1572:1581	arg1	strategy					1583:1590	an integrated strategy	1569:1590	an integrated strategy for rapid and accurate SERS analysis in food safety issues	1569:1649	The SERS method based on Sep/CTs/AgNPs membrane substrate provided an integrated strategy for rapid and accurate SERS analysis in food safety issues.					
35469076	3	58	theme	Raman	671:675	arg1	signals					677:683	the Raman signals	667:683	the Raman signals of target molecules	667:703	The grown AgNPs can provide abundant hot spots and plasmonic areas to amplify the Raman signals of target molecules effectively.					
35469076	4	59	theme	membrane	722:729	arg1	substrate					731:739	The membrane substrate	718:739	The membrane substrate	718:739	The membrane substrate exhibited good stability with relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively, by detecting sulfamethoxazole.					
35469076	4	60	dep	batch	825:829	arg1	substrate					862:870	membrane substrate	853:870	membrane substrate	853:870	The membrane substrate exhibited good stability with relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively, by detecting sulfamethoxazole.					
35469076	3	61	theme	molecules	695:703	arg1	signals					677:683	the Raman signals	667:683	the Raman signals of target molecules	667:703	The grown AgNPs can provide abundant hot spots and plasmonic areas to amplify the Raman signals of target molecules effectively.					
35469076	9	62	theme	SERS	1615:1618	arg1	analysis					1620:1627	rapid and accurate SERS analysis	1596:1627	rapid and accurate SERS analysis in food safety issues	1596:1649	The SERS method based on Sep/CTs/AgNPs membrane substrate provided an integrated strategy for rapid and accurate SERS analysis in food safety issues.					
35469076	6	63	theme	SERS	1124:1127	arg1	method					1129:1134	The established SERS method	1108:1134	The established SERS method	1108:1134	The established SERS method was finally applied to the quantification of sulfamethoxazole in animal-derived food samples.					
35469076	4	64	theme	relative	771:778	arg1	deviations					789:798	relative standard deviations	771:798	relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively	771:884	The membrane substrate exhibited good stability with relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively, by detecting sulfamethoxazole.					
35469076	0	65	theme	enrichment	83:92	arg1	determination					132:144	enrichment and surface-enhanced Raman scattering determination	83:144	enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food	83:187	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	1	66	theme	sulfamethoxazole	385:400	arg1	substrate					257:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	1	66	theme	sulfamethoxazole	385:400	arg1	determination					368:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination	276:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one	276:411	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	9	67	theme	safety	1637:1642	arg1	issues					1644:1649	food safety issues	1632:1649	food safety issues	1632:1649	The SERS method based on Sep/CTs/AgNPs membrane substrate provided an integrated strategy for rapid and accurate SERS analysis in food safety issues.					
35469076	8	68	theme	analysis	1431:1438	arg1	process					1420:1426	The whole process	1410:1426	The whole process of analysis	1410:1438	The whole process of analysis can be finished within 25 min with recoveries of 89.3-102.2%.					
35469076	0	69	theme	surface-enhanced	98:113	arg1	scattering					121:130	surface-enhanced Raman scattering	98:130	surface-enhanced Raman scattering	98:130	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	5	70	with	used for	980:987	arg1	range					1041:1045	a linear range	1032:1045	a linear range of 0.05-2.0 mg/L	1032:1062	The SERS method based on Sep/CTs/AgNPs membrane substrate was used for the determination of sulfamethoxazole with a linear range of 0.05-2.0 mg/L, and the limit of detection was 0.020 mg/L.					
35469076	0	71	theme	scattering	121:130	arg1	determination					132:144	enrichment and surface-enhanced Raman scattering determination	83:144	enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food	83:187	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	1	72	theme	membrane	248:255	arg1	substrate					257:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	1	72	theme	membrane	248:255	arg1	determination					368:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination	276:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one	276:411	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	0	73	theme	membrane	9:16	arg1	composite					18:26	Flexible membrane composite	0:26	Flexible membrane composite	0:26	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	5	74	theme	linear	1034:1039	arg1	range					1041:1045	a linear range	1032:1045	a linear range of 0.05-2.0 mg/L	1032:1062	The SERS method based on Sep/CTs/AgNPs membrane substrate was used for the determination of sulfamethoxazole with a linear range of 0.05-2.0 mg/L, and the limit of detection was 0.020 mg/L.					
35469076	4	75	theme	%	815:815	arg1	deviations					789:798	relative standard deviations	771:798	relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively	771:884	The membrane substrate exhibited good stability with relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively, by detecting sulfamethoxazole.					
35469076	1	76	theme	surface-enhanced	327:342	arg1	scattering					350:359	surface-enhanced Raman scattering	327:359	surface-enhanced Raman scattering	327:359	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	4	77	theme	same	820:823	arg1	batch					825:829	same batch	820:829	same batch	820:829	The membrane substrate exhibited good stability with relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively, by detecting sulfamethoxazole.					
35469076	1	78	theme	scattering	350:359	arg1	substrate					257:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	1	78	theme	scattering	350:359	arg1	determination					368:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination	276:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one	276:411	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	0	79	link	animal-derived	169:182	arg1	food					184:187	animal-derived food	169:187	animal-derived food	169:187	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	7	80	theme	%	1407:1407	arg1	error					1395:1399	relative error	1386:1399	relative error of 5.3%	1386:1407	Sulfamethoxazole was actually found in crucian sample with 12.4 μg/kg, and the result was confirmed by a high-performance liquid chromatography method with relative error of 5.3%.					
35469076	8	81	theme	%	1499:1499	arg1	recoveries					1475:1484	recoveries	1475:1484	recoveries of 89.3-102.2%	1475:1499	The whole process of analysis can be finished within 25 min with recoveries of 89.3-102.2%.					
35469076	2	82	theme	rapid	476:480	arg1	separation					482:491	rapid separation	476:491	rapid separation	476:491	The Sep/CTs/AgNPs membrane substrate possessed the ability of rapid separation and enrichment to simplify the process for pretreatment and improve the efficiency of analysis.					
35469076	0	83	theme	silver	57:62	arg1	sepiolite/chitosan/					37:55	sepiolite/chitosan/	37:55	sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food	37:187	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	0	83	theme	silver	57:62	arg1	nanoparticles					64:76	silver nanoparticles	57:76	silver nanoparticles	57:76	Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.					
35469076	7	84	theme	relative	1386:1393	arg1	error					1395:1399	relative error	1386:1399	relative error of 5.3%	1386:1407	Sulfamethoxazole was actually found in crucian sample with 12.4 μg/kg, and the result was confirmed by a high-performance liquid chromatography method with relative error of 5.3%.					
35469076	1	85	theme	developed for	276:288	arg1	substrate					257:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate	190:265	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	1	85	theme	developed for	276:288	arg1	determination					368:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination	276:380	developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one	276:411	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	6	86	theme	food	1216:1219	arg1	samples					1221:1227	animal-derived food samples	1201:1227	animal-derived food samples	1201:1227	The established SERS method was finally applied to the quantification of sulfamethoxazole in animal-derived food samples.					
35469076	9	87	theme	SERS	1506:1509	arg1	method					1511:1516	The SERS method	1502:1516	The SERS method based on Sep/CTs/AgNPs membrane substrate	1502:1558	The SERS method based on Sep/CTs/AgNPs membrane substrate provided an integrated strategy for rapid and accurate SERS analysis in food safety issues.					
35469076	2	88	theme	membrane	432:439	arg1	substrate					441:449	The Sep/CTs/AgNPs membrane substrate	414:449	The Sep/CTs/AgNPs membrane substrate	414:449	The Sep/CTs/AgNPs membrane substrate possessed the ability of rapid separation and enrichment to simplify the process for pretreatment and improve the efficiency of analysis.					
35469076	1	89	theme	fast	294:297	arg1	SERS					362:365	SERS	362:365	SERS	362:365	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35469076	1	89	theme	fast	294:297	arg1	separation					299:308	the fast separation	290:308	the fast separation	290:308	A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one.					
35963174	6	0	theme	adsorption	809:818	arg1	experiments					820:830	batch adsorption experiments	803:830	batch adsorption experiments	803:830	The elimination capacity of SA/LS/PRC for Cu2+, Ni2+, and Methylene blue (MB) was researched through batch adsorption experiments.					
35963174	1	1	theme	heavy	138:142	arg1	ions					150:153	heavy metal ions	138:153	heavy metal ions	138:153	The pollution of heavy metal ions and organic dyes seriously endangers human health and causes acute environmental problems.					
35963174	8	2	theme	maximum	1023:1029	arg1	1245.27					1092:1098	1245.27	1092:1098	1245.27	1092:1098	The maximum adsorption capacities of SA/LS/PRC for Cu2+, Ni2+ and MB are 1245.27, 1239.47 and 627.29 mg g-1, respectively, and the adsorption performance is better than those reported in most literatures.					
35963174	8	2	theme	maximum	1023:1029	arg1	capacities					1042:1051	The maximum adsorption capacities	1019:1051	The maximum adsorption capacities of SA/LS/PRC for Cu2+, Ni2+ and MB	1019:1086	The maximum adsorption capacities of SA/LS/PRC for Cu2+, Ni2+ and MB are 1245.27, 1239.47 and 627.29 mg g-1, respectively, and the adsorption performance is better than those reported in most literatures.					
35963174	6	3	theme	batch	803:807	arg1	experiments					820:830	batch adsorption experiments	803:830	batch adsorption experiments	803:830	The elimination capacity of SA/LS/PRC for Cu2+, Ni2+, and Methylene blue (MB) was researched through batch adsorption experiments.					
35963174	9	4	dep	adsorption	1292:1301	arg1	experience					1303:1312	experience	1303:1312	experience	1303:1312	The interaction of Cu2+, Ni2+, and MB were studied via binary mixed adsorption experience.					
35963174	1	5	theme	metal	144:148	arg1	ions					150:153	heavy metal ions	138:153	heavy metal ions	138:153	The pollution of heavy metal ions and organic dyes seriously endangers human health and causes acute environmental problems.					
35963174	6	6	theme	SA/LS/PRC	730:738	arg1	capacity					718:725	The elimination capacity	702:725	The elimination capacity of SA/LS/PRC for Cu2+, Ni2+, and Methylene blue (MB)	702:778	The elimination capacity of SA/LS/PRC for Cu2+, Ni2+, and Methylene blue (MB) was researched through batch adsorption experiments.					
35963174	11	7	theme	adsorption	1422:1431	arg1	capacity					1433:1440	The adsorption capacity	1418:1440	The adsorption capacity of SA/LS/PRC	1418:1453	The adsorption capacity of SA/LS/PRC kept above 81% after five cycles.					
35963174	5	8	theme	Zeta	646:649	arg1	potential					651:659	Zeta potential	646:659	Zeta potential	646:659	Meanwhile, SEM, FTIR, TGA, and Zeta potential were used to characterize the adsorbent.					
35963174	11	9	theme	SA/LS/PRC	1445:1453	arg1	capacity					1433:1440	The adsorption capacity	1418:1440	The adsorption capacity of SA/LS/PRC	1418:1453	The adsorption capacity of SA/LS/PRC kept above 81% after five cycles.					
35963174	4	10	theme	sodium	526:531	arg1	alginate					533:540	sodium alginate	526:540	sodium alginate	526:540	By means of radical polymerization, PEI-modified reed carbon (PRC), sodium alginate and lysine were made into carbon-composite acrylic hydrogel (SA/LS/PRC).					
35963174	10	11	theme	removal	1381:1387	arg1	mechanisms					1389:1398	the major removal mechanisms	1371:1398	the major removal mechanisms for contaminants	1371:1415	Surface complexation and electrostatic interactions are the major removal mechanisms for contaminants.					
35963174	10	11	theme	removal	1381:1387	arg1	interactions					1354:1365	electrostatic interactions	1340:1365	electrostatic interactions	1340:1365	Surface complexation and electrostatic interactions are the major removal mechanisms for contaminants.					
35963174	10	11	theme	removal	1381:1387	arg1	complexation					1323:1334	Surface complexation	1315:1334	Surface complexation	1315:1334	Surface complexation and electrostatic interactions are the major removal mechanisms for contaminants.					
35963174	7	12	theme	SA/LS/PRC	860:868	arg1	suitable					878:885	suitable	878:885	suitable	878:885	The adsorption behavior of SA/LS/PRC is more suitable for the pseudo-second-order kinetic model of chemical removal of pollutants and the Langmuir model relying on monolayer adsorption.					
35963174	7	12	theme	SA/LS/PRC	860:868	arg1	behavior					848:855	The adsorption behavior	833:855	The adsorption behavior of SA/LS/PRC	833:868	The adsorption behavior of SA/LS/PRC is more suitable for the pseudo-second-order kinetic model of chemical removal of pollutants and the Langmuir model relying on monolayer adsorption.					
35963174	10	13	theme	electrostatic	1340:1352	arg1	mechanisms					1389:1398	the major removal mechanisms	1371:1398	the major removal mechanisms for contaminants	1371:1415	Surface complexation and electrostatic interactions are the major removal mechanisms for contaminants.					
35963174	10	13	theme	electrostatic	1340:1352	arg1	interactions					1354:1365	electrostatic interactions	1340:1365	electrostatic interactions	1340:1365	Surface complexation and electrostatic interactions are the major removal mechanisms for contaminants.					
35963174	10	13	theme	electrostatic	1340:1352	arg1	complexation					1323:1334	Surface complexation	1315:1334	Surface complexation	1315:1334	Surface complexation and electrostatic interactions are the major removal mechanisms for contaminants.					
35963174	3	14	theme	reed	393:396	arg1	ORC					406:408	ORC	406:408	ORC	406:408	In this work, the original reed carbon (ORC) was functionalized by polyethyleneimine (PEI).					
35963174	3	14	theme	reed	393:396	arg1	carbon					398:403	the original reed carbon	380:403	the original reed carbon (ORC)	380:409	In this work, the original reed carbon (ORC) was functionalized by polyethyleneimine (PEI).					
35963174	1	15	theme	ions	150:153	arg1	pollution					125:133	The pollution	121:133	The pollution of heavy metal ions and organic dyes	121:170	The pollution of heavy metal ions and organic dyes seriously endangers human health and causes acute environmental problems.					
35963174	0	16	theme	blue	94:97	arg1	methylene					84:92	methylene blue	84:97	methylene blue in aqueous solutions	84:118	Preparation of modified reed carbon composite hydrogels for trapping Cu2+, Ni2+ and methylene blue in aqueous solutions.					
35963174	10	17	theme	major	1375:1379	arg1	mechanisms					1389:1398	the major removal mechanisms	1371:1398	the major removal mechanisms for contaminants	1371:1415	Surface complexation and electrostatic interactions are the major removal mechanisms for contaminants.					
35963174	10	17	theme	major	1375:1379	arg1	interactions					1354:1365	electrostatic interactions	1340:1365	electrostatic interactions	1340:1365	Surface complexation and electrostatic interactions are the major removal mechanisms for contaminants.					
35963174	10	17	theme	major	1375:1379	arg1	complexation					1323:1334	Surface complexation	1315:1334	Surface complexation	1315:1334	Surface complexation and electrostatic interactions are the major removal mechanisms for contaminants.					
35963174	6	18	theme	elimination	706:716	arg1	capacity					718:725	The elimination capacity	702:725	The elimination capacity of SA/LS/PRC for Cu2+, Ni2+, and Methylene blue (MB)	702:778	The elimination capacity of SA/LS/PRC for Cu2+, Ni2+, and Methylene blue (MB) was researched through batch adsorption experiments.					
35963174	12	19	theme	heavy	1580:1584	arg1	ions					1592:1595	heavy metal ions	1580:1595	heavy metal ions	1580:1595	The SA/LS/PRC green hydrogel can be effectively applied in the mixed adsorption process of heavy metal ions and the removal of dyes.					
35963174	9	20	theme	MB	1259:1260	arg1	interaction					1228:1238	The interaction	1224:1238	The interaction of Cu2+, Ni2+, and MB	1224:1260	The interaction of Cu2+, Ni2+, and MB were studied via binary mixed adsorption experience.					
35963174	8	21	theme	most	1206:1209	arg1	literatures					1211:1221	most literatures	1206:1221	most literatures	1206:1221	The maximum adsorption capacities of SA/LS/PRC for Cu2+, Ni2+ and MB are 1245.27, 1239.47 and 627.29 mg g-1, respectively, and the adsorption performance is better than those reported in most literatures.					
35963174	10	22	theme	Surface	1315:1321	arg1	complexation					1323:1334	Surface complexation	1315:1334	Surface complexation	1315:1334	Surface complexation and electrostatic interactions are the major removal mechanisms for contaminants.					
35963174	10	22	theme	Surface	1315:1321	arg1	interactions					1354:1365	electrostatic interactions	1340:1365	electrostatic interactions	1340:1365	Surface complexation and electrostatic interactions are the major removal mechanisms for contaminants.					
35963174	10	22	theme	Surface	1315:1321	arg1	mechanisms					1389:1398	the major removal mechanisms	1371:1398	the major removal mechanisms for contaminants	1371:1415	Surface complexation and electrostatic interactions are the major removal mechanisms for contaminants.					
35963174	1	23	theme	organic	159:165	arg1	dyes					167:170	organic dyes	159:170	organic dyes	159:170	The pollution of heavy metal ions and organic dyes seriously endangers human health and causes acute environmental problems.					
35963174	5	24	used	used	666:669	arg2	potential					651:659	Zeta potential	646:659	Zeta potential	646:659	Meanwhile, SEM, FTIR, TGA, and Zeta potential were used to characterize the adsorbent.					
35963174	5	24	used	used	666:669	arg2	Meanwhile					615:623	Meanwhile	615:623	Meanwhile	615:623	Meanwhile, SEM, FTIR, TGA, and Zeta potential were used to characterize the adsorbent.					
35963174	5	24	used	used	666:669	arg2	TGA					637:639	TGA	637:639	TGA	637:639	Meanwhile, SEM, FTIR, TGA, and Zeta potential were used to characterize the adsorbent.					
35963174	5	24	used	used	666:669	arg2	FTIR					631:634	FTIR	631:634	FTIR	631:634	Meanwhile, SEM, FTIR, TGA, and Zeta potential were used to characterize the adsorbent.					
35963174	5	24	used	used	666:669	arg2	SEM					626:628	SEM	626:628	SEM	626:628	Meanwhile, SEM, FTIR, TGA, and Zeta potential were used to characterize the adsorbent.					
35963174	1	25	theme	dyes	167:170	arg1	pollution					125:133	The pollution	121:133	The pollution of heavy metal ions and organic dyes	121:170	The pollution of heavy metal ions and organic dyes seriously endangers human health and causes acute environmental problems.					
35963174	0	26	theme	reed	24:27	arg1	composite					36:44	modified reed carbon composite	15:44	modified reed carbon composite	15:44	Preparation of modified reed carbon composite hydrogels for trapping Cu2+, Ni2+ and methylene blue in aqueous solutions.					
35963174	4	27	theme	reed	507:510	arg1	carbon					512:517	PEI-modified reed carbon	494:517	PEI-modified reed carbon (PRC)	494:523	By means of radical polymerization, PEI-modified reed carbon (PRC), sodium alginate and lysine were made into carbon-composite acrylic hydrogel (SA/LS/PRC).					
35963174	4	27	theme	reed	507:510	arg1	PRC					520:522	PRC	520:522	PRC	520:522	By means of radical polymerization, PEI-modified reed carbon (PRC), sodium alginate and lysine were made into carbon-composite acrylic hydrogel (SA/LS/PRC).					
35963174	4	28	theme	carbon-composite	568:583	arg1	SA/LS/PRC					603:611	SA/LS/PRC	603:611	SA/LS/PRC	603:611	By means of radical polymerization, PEI-modified reed carbon (PRC), sodium alginate and lysine were made into carbon-composite acrylic hydrogel (SA/LS/PRC).					
35963174	4	28	theme	carbon-composite	568:583	arg1	hydrogel					593:600	carbon-composite acrylic hydrogel	568:600	carbon-composite acrylic hydrogel (SA/LS/PRC)	568:612	By means of radical polymerization, PEI-modified reed carbon (PRC), sodium alginate and lysine were made into carbon-composite acrylic hydrogel (SA/LS/PRC).					
35963174	0	29	theme	modified	15:22	arg1	composite					36:44	modified reed carbon composite	15:44	modified reed carbon composite	15:44	Preparation of modified reed carbon composite hydrogels for trapping Cu2+, Ni2+ and methylene blue in aqueous solutions.					
35963174	0	30	theme	aqueous	102:108	arg1	solutions					110:118	aqueous solutions	102:118	aqueous solutions	102:118	Preparation of modified reed carbon composite hydrogels for trapping Cu2+, Ni2+ and methylene blue in aqueous solutions.					
35963174	0	31	from	Ni2+	75:78	arg1	solutions					110:118	aqueous solutions	102:118	aqueous solutions	102:118	Preparation of modified reed carbon composite hydrogels for trapping Cu2+, Ni2+ and methylene blue in aqueous solutions.					
35963174	12	32	theme	mixed	1552:1556	arg1	process					1569:1575	the mixed adsorption process	1548:1575	the mixed adsorption process of heavy metal ions	1548:1595	The SA/LS/PRC green hydrogel can be effectively applied in the mixed adsorption process of heavy metal ions and the removal of dyes.					
35963174	0	33	theme	composite	36:44	arg1	Preparation					0:10	Preparation	0:10	Preparation of modified reed carbon composite	0:44	Preparation of modified reed carbon composite hydrogels for trapping Cu2+, Ni2+ and methylene blue in aqueous solutions.					
35963174	9	34	theme	Ni2+	1249:1252	arg1	interaction					1228:1238	The interaction	1224:1238	The interaction of Cu2+, Ni2+, and MB	1224:1260	The interaction of Cu2+, Ni2+, and MB were studied via binary mixed adsorption experience.					
35963174	6	35	theme	blue	770:773	arg1	Methylene					760:768	Methylene blue	760:773	Methylene blue (MB)	760:778	The elimination capacity of SA/LS/PRC for Cu2+, Ni2+, and Methylene blue (MB) was researched through batch adsorption experiments.					
35963174	6	35	theme	blue	770:773	arg1	MB					776:777	MB	776:777	MB	776:777	The elimination capacity of SA/LS/PRC for Cu2+, Ni2+, and Methylene blue (MB) was researched through batch adsorption experiments.					
35963174	1	36	theme	human	192:196	arg1	health					198:203	human health	192:203	human health	192:203	The pollution of heavy metal ions and organic dyes seriously endangers human health and causes acute environmental problems.					
35963174	0	37	theme	carbon	29:34	arg1	composite					36:44	modified reed carbon composite	15:44	modified reed carbon composite	15:44	Preparation of modified reed carbon composite hydrogels for trapping Cu2+, Ni2+ and methylene blue in aqueous solutions.					
35963174	12	38	theme	adsorption	1558:1567	arg1	process					1569:1575	the mixed adsorption process	1548:1575	the mixed adsorption process of heavy metal ions	1548:1595	The SA/LS/PRC green hydrogel can be effectively applied in the mixed adsorption process of heavy metal ions and the removal of dyes.					
35963174	3	39	theme	original	384:391	arg1	ORC					406:408	ORC	406:408	ORC	406:408	In this work, the original reed carbon (ORC) was functionalized by polyethyleneimine (PEI).					
35963174	3	39	theme	original	384:391	arg1	carbon					398:403	the original reed carbon	380:403	the original reed carbon (ORC)	380:409	In this work, the original reed carbon (ORC) was functionalized by polyethyleneimine (PEI).					
35963174	0	40	from	Cu2+	69:72	arg1	solutions					110:118	aqueous solutions	102:118	aqueous solutions	102:118	Preparation of modified reed carbon composite hydrogels for trapping Cu2+, Ni2+ and methylene blue in aqueous solutions.					
35963174	9	41	theme	binary	1279:1284	arg1	adsorption					1292:1301	binary mixed adsorption	1279:1301	binary mixed adsorption experience	1279:1312	The interaction of Cu2+, Ni2+, and MB were studied via binary mixed adsorption experience.					
35963174	4	42	theme	acrylic	585:591	arg1	SA/LS/PRC					603:611	SA/LS/PRC	603:611	SA/LS/PRC	603:611	By means of radical polymerization, PEI-modified reed carbon (PRC), sodium alginate and lysine were made into carbon-composite acrylic hydrogel (SA/LS/PRC).					
35963174	4	42	theme	acrylic	585:591	arg1	hydrogel					593:600	carbon-composite acrylic hydrogel	568:600	carbon-composite acrylic hydrogel (SA/LS/PRC)	568:612	By means of radical polymerization, PEI-modified reed carbon (PRC), sodium alginate and lysine were made into carbon-composite acrylic hydrogel (SA/LS/PRC).					
35963174	4	43	theme	radical	470:476	arg1	polymerization					478:491	radical polymerization	470:491	radical polymerization	470:491	By means of radical polymerization, PEI-modified reed carbon (PRC), sodium alginate and lysine were made into carbon-composite acrylic hydrogel (SA/LS/PRC).					
35963174	7	44	theme	pollutants	952:961	arg1	removal					941:947	chemical removal	932:947	chemical removal of pollutants	932:961	The adsorption behavior of SA/LS/PRC is more suitable for the pseudo-second-order kinetic model of chemical removal of pollutants and the Langmuir model relying on monolayer adsorption.					
35963174	9	45	theme	Cu2+	1243:1246	arg1	interaction					1228:1238	The interaction	1224:1238	The interaction of Cu2+, Ni2+, and MB	1224:1260	The interaction of Cu2+, Ni2+, and MB were studied via binary mixed adsorption experience.					
35963174	7	46	theme	chemical	932:939	arg1	removal					941:947	chemical removal	932:947	chemical removal of pollutants	932:961	The adsorption behavior of SA/LS/PRC is more suitable for the pseudo-second-order kinetic model of chemical removal of pollutants and the Langmuir model relying on monolayer adsorption.					
35963174	12	47	theme	green	1503:1507	arg1	hydrogel					1509:1516	The SA/LS/PRC green hydrogel	1489:1516	The SA/LS/PRC green hydrogel	1489:1516	The SA/LS/PRC green hydrogel can be effectively applied in the mixed adsorption process of heavy metal ions and the removal of dyes.					
35963174	7	48	theme	Langmuir	971:978	arg1	model					980:984	the Langmuir model	967:984	the Langmuir model relying on monolayer adsorption	967:1016	The adsorption behavior of SA/LS/PRC is more suitable for the pseudo-second-order kinetic model of chemical removal of pollutants and the Langmuir model relying on monolayer adsorption.					
35963174	4	49	theme	PEI-modified	494:505	arg1	carbon					512:517	PEI-modified reed carbon	494:517	PEI-modified reed carbon (PRC)	494:523	By means of radical polymerization, PEI-modified reed carbon (PRC), sodium alginate and lysine were made into carbon-composite acrylic hydrogel (SA/LS/PRC).					
35963174	4	49	theme	PEI-modified	494:505	arg1	PRC					520:522	PRC	520:522	PRC	520:522	By means of radical polymerization, PEI-modified reed carbon (PRC), sodium alginate and lysine were made into carbon-composite acrylic hydrogel (SA/LS/PRC).					
35963174	12	50	theme	metal	1586:1590	arg1	ions					1592:1595	heavy metal ions	1580:1595	heavy metal ions	1580:1595	The SA/LS/PRC green hydrogel can be effectively applied in the mixed adsorption process of heavy metal ions and the removal of dyes.					
35963174	7	51	theme	kinetic	915:921	arg1	model					923:927	the pseudo-second-order kinetic model	891:927	the pseudo-second-order kinetic model of chemical removal of pollutants	891:961	The adsorption behavior of SA/LS/PRC is more suitable for the pseudo-second-order kinetic model of chemical removal of pollutants and the Langmuir model relying on monolayer adsorption.					
35963174	12	52	theme	SA/LS/PRC	1493:1501	arg1	hydrogel					1509:1516	The SA/LS/PRC green hydrogel	1489:1516	The SA/LS/PRC green hydrogel	1489:1516	The SA/LS/PRC green hydrogel can be effectively applied in the mixed adsorption process of heavy metal ions and the removal of dyes.					
35963174	7	53	theme	removal	941:947	arg1	model					923:927	the pseudo-second-order kinetic model	891:927	the pseudo-second-order kinetic model of chemical removal of pollutants	891:961	The adsorption behavior of SA/LS/PRC is more suitable for the pseudo-second-order kinetic model of chemical removal of pollutants and the Langmuir model relying on monolayer adsorption.					
35963174	7	53	theme	removal	941:947	arg1	model					980:984	the Langmuir model	967:984	the Langmuir model relying on monolayer adsorption	967:1016	The adsorption behavior of SA/LS/PRC is more suitable for the pseudo-second-order kinetic model of chemical removal of pollutants and the Langmuir model relying on monolayer adsorption.					
35963174	7	54	theme	adsorption	837:846	arg1	suitable					878:885	suitable	878:885	suitable	878:885	The adsorption behavior of SA/LS/PRC is more suitable for the pseudo-second-order kinetic model of chemical removal of pollutants and the Langmuir model relying on monolayer adsorption.					
35963174	7	54	theme	adsorption	837:846	arg1	behavior					848:855	The adsorption behavior	833:855	The adsorption behavior of SA/LS/PRC	833:868	The adsorption behavior of SA/LS/PRC is more suitable for the pseudo-second-order kinetic model of chemical removal of pollutants and the Langmuir model relying on monolayer adsorption.					
35963174	2	55	theme	adsorption	250:259	arg1	method					261:266	The adsorption method	246:266	The adsorption method	246:266	The adsorption method is extensively adopted in the treatment of water pollution which is more harmful to human health.					
35963174	8	56	theme	adsorption	1150:1159	arg1	better					1176:1181	better	1176:1181	better	1176:1181	The maximum adsorption capacities of SA/LS/PRC for Cu2+, Ni2+ and MB are 1245.27, 1239.47 and 627.29 mg g-1, respectively, and the adsorption performance is better than those reported in most literatures.					
35963174	8	56	theme	adsorption	1150:1159	arg1	performance					1161:1171	the adsorption performance	1146:1171	the adsorption performance	1146:1171	The maximum adsorption capacities of SA/LS/PRC for Cu2+, Ni2+ and MB are 1245.27, 1239.47 and 627.29 mg g-1, respectively, and the adsorption performance is better than those reported in most literatures.					
35963174	2	57	theme	pollution	317:325	arg1	treatment					298:306	the treatment	294:306	the treatment of water pollution which is more harmful to human health	294:363	The adsorption method is extensively adopted in the treatment of water pollution which is more harmful to human health.					
35963174	8	58	theme	adsorption	1031:1040	arg1	1245.27					1092:1098	1245.27	1092:1098	1245.27	1092:1098	The maximum adsorption capacities of SA/LS/PRC for Cu2+, Ni2+ and MB are 1245.27, 1239.47 and 627.29 mg g-1, respectively, and the adsorption performance is better than those reported in most literatures.					
35963174	8	58	theme	adsorption	1031:1040	arg1	capacities					1042:1051	The maximum adsorption capacities	1019:1051	The maximum adsorption capacities of SA/LS/PRC for Cu2+, Ni2+ and MB	1019:1086	The maximum adsorption capacities of SA/LS/PRC for Cu2+, Ni2+ and MB are 1245.27, 1239.47 and 627.29 mg g-1, respectively, and the adsorption performance is better than those reported in most literatures.					
35963174	0	59	from	methylene	84:92	arg1	solutions					110:118	aqueous solutions	102:118	aqueous solutions	102:118	Preparation of modified reed carbon composite hydrogels for trapping Cu2+, Ni2+ and methylene blue in aqueous solutions.					
35963174	2	60	theme	water	311:315	arg1	pollution					317:325	water pollution	311:325	water pollution which is more harmful to human health	311:363	The adsorption method is extensively adopted in the treatment of water pollution which is more harmful to human health.					
35963174	7	61	theme	monolayer	997:1005	arg1	adsorption					1007:1016	monolayer adsorption	997:1016	monolayer adsorption	997:1016	The adsorption behavior of SA/LS/PRC is more suitable for the pseudo-second-order kinetic model of chemical removal of pollutants and the Langmuir model relying on monolayer adsorption.					
35963174	8	62	theme	SA/LS/PRC	1056:1064	arg1	1245.27					1092:1098	1245.27	1092:1098	1245.27	1092:1098	The maximum adsorption capacities of SA/LS/PRC for Cu2+, Ni2+ and MB are 1245.27, 1239.47 and 627.29 mg g-1, respectively, and the adsorption performance is better than those reported in most literatures.					
35963174	8	62	theme	SA/LS/PRC	1056:1064	arg1	capacities					1042:1051	The maximum adsorption capacities	1019:1051	The maximum adsorption capacities of SA/LS/PRC for Cu2+, Ni2+ and MB	1019:1086	The maximum adsorption capacities of SA/LS/PRC for Cu2+, Ni2+ and MB are 1245.27, 1239.47 and 627.29 mg g-1, respectively, and the adsorption performance is better than those reported in most literatures.					
35963174	7	63	theme	pseudo-second-order	895:913	arg1	model					923:927	the pseudo-second-order kinetic model	891:927	the pseudo-second-order kinetic model of chemical removal of pollutants	891:961	The adsorption behavior of SA/LS/PRC is more suitable for the pseudo-second-order kinetic model of chemical removal of pollutants and the Langmuir model relying on monolayer adsorption.					
35963174	2	64	theme	human	352:356	arg1	health					358:363	human health	352:363	human health	352:363	The adsorption method is extensively adopted in the treatment of water pollution which is more harmful to human health.					
35963174	12	65	theme	dyes	1616:1619	arg1	process					1569:1575	the mixed adsorption process	1548:1575	the mixed adsorption process of heavy metal ions	1548:1595	The SA/LS/PRC green hydrogel can be effectively applied in the mixed adsorption process of heavy metal ions and the removal of dyes.					
35963174	12	65	theme	dyes	1616:1619	arg1	removal					1605:1611	the removal	1601:1611	the removal of dyes	1601:1619	The SA/LS/PRC green hydrogel can be effectively applied in the mixed adsorption process of heavy metal ions and the removal of dyes.					
35963174	1	66	theme	acute	216:220	arg1	problems					236:243	acute environmental problems	216:243	acute environmental problems	216:243	The pollution of heavy metal ions and organic dyes seriously endangers human health and causes acute environmental problems.					
35963174	0	67	theme	trapping	60:67	arg1	Cu2+					69:72	trapping Cu2+	60:72	trapping Cu2+	60:72	Preparation of modified reed carbon composite hydrogels for trapping Cu2+, Ni2+ and methylene blue in aqueous solutions.					
35963174	9	68	theme	mixed	1286:1290	arg1	adsorption					1292:1301	binary mixed adsorption	1279:1301	binary mixed adsorption experience	1279:1312	The interaction of Cu2+, Ni2+, and MB were studied via binary mixed adsorption experience.					
35963174	12	69	theme	ions	1592:1595	arg1	process					1569:1575	the mixed adsorption process	1548:1575	the mixed adsorption process of heavy metal ions	1548:1595	The SA/LS/PRC green hydrogel can be effectively applied in the mixed adsorption process of heavy metal ions and the removal of dyes.					
35963174	12	69	theme	ions	1592:1595	arg1	removal					1605:1611	the removal	1601:1611	the removal of dyes	1601:1619	The SA/LS/PRC green hydrogel can be effectively applied in the mixed adsorption process of heavy metal ions and the removal of dyes.					
35963174	1	70	theme	environmental	222:234	arg1	problems					236:243	acute environmental problems	216:243	acute environmental problems	216:243	The pollution of heavy metal ions and organic dyes seriously endangers human health and causes acute environmental problems.					
35598510	0	0	theme	cationic	101:108	arg1	dyes					110:113	anionic and cationic dyes	89:113	anionic and cationic dyes	89:113	Mesoporous crosslinked chitosan-activated clinoptilolite biocomposite for the removal of anionic and cationic dyes.					
35598510	7	1	theme	mechanism	1126:1134	arg1	studies					1136:1142	The proposed mechanism studies	1113:1142	The proposed mechanism studies	1113:1142	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	2	2	theme	FTIR	373:376	arg1	techniques					392:401	FTIR, SEM, and BET techniques	373:401	FTIR, SEM, and BET techniques	373:401	Structural analysis of the CS-GA/ACP composite beads was performed using FTIR, SEM, and BET techniques.					
35598510	5	3	theme	pseudo-second-order	807:825	arg1	kinetics					827:834	The pseudo-second-order kinetics	803:834	The pseudo-second-order kinetics	803:834	The pseudo-second-order kinetics could better describe the adsorption process than the pseudo-first-order kinetics, and the Langmuir isotherms model agreed well with the experimental data.					
35598510	9	4	theme	industrial	1614:1623	arg1	applications					1625:1636	industrial applications	1614:1636	industrial applications	1614:1636	Overall, the CS-GA/ACP showed an excellent performance for dyes removal in aqueous solution and could be a practical candidate for industrial applications.					
35598510	6	5	theme	adsorption	1004:1013	arg1	180.59 mg/g					1062:1072	180.59 mg/g	1062:1072	180.59 mg/g	1062:1072	The maximum adsorption capacities of the CS-GA/ACP for CR and MB were 180.59 mg/g and 143.67 mg/g at 25 ℃, respectively.					
35598510	6	5	theme	adsorption	1004:1013	arg1	capacities					1015:1024	The maximum adsorption capacities	992:1024	The maximum adsorption capacities of the CS-GA/ACP for CR and MB	992:1055	The maximum adsorption capacities of the CS-GA/ACP for CR and MB were 180.59 mg/g and 143.67 mg/g at 25 ℃, respectively.					
35598510	7	6	theme	adsorbent	1193:1201	arg1	molecules					1211:1219	the adsorbent and dye molecules	1189:1219	molecules	1211:1219	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	7	theme	n-π	1300:1302	arg1	H-bonding					1259:1267	H-bonding	1259:1267	H-bonding	1259:1267	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	7	theme	n-π	1300:1302	arg1	interaction					1304:1314	n-π interaction	1300:1314	n-π interaction	1300:1314	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	7	theme	n-π	1300:1302	arg1	interaction					1169:1179	the possible interaction	1156:1179	the possible interaction between the adsorbent and dye molecules	1156:1219	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	7	theme	n-π	1300:1302	arg1	Yoshida					1226:1232	Yoshida	1226:1232	Yoshida	1226:1232	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	7	theme	n-π	1300:1302	arg1	H-bonding					1234:1242	H-bonding	1234:1242	H-bonding	1234:1242	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	7	theme	n-π	1300:1302	arg1	interaction					1284:1294	electrostatic interaction	1270:1294	electrostatic interaction	1270:1294	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	4	8	theme	composite	768:776	arg1	beads					778:782	the CS-GA/ACP composite beads	754:782	the CS-GA/ACP composite beads	754:782	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	9	9	theme	dyes	1542:1545	arg1	removal					1547:1553	dyes removal	1542:1553	dyes removal	1542:1553	Overall, the CS-GA/ACP showed an excellent performance for dyes removal in aqueous solution and could be a practical candidate for industrial applications.					
35598510	2	10	theme	Structural	300:309	arg1	analysis					311:318	Structural analysis	300:318	Structural analysis of the CS-GA/ACP composite beads	300:351	Structural analysis of the CS-GA/ACP composite beads was performed using FTIR, SEM, and BET techniques.					
35598510	5	11	theme	adsorption	862:871	arg1	process					873:879	the adsorption process	858:879	the adsorption process than the pseudo-first-order kinetics	858:916	The pseudo-second-order kinetics could better describe the adsorption process than the pseudo-first-order kinetics, and the Langmuir isotherms model agreed well with the experimental data.					
35598510	4	12	theme	loading	552:558	arg1	effects					538:544	The effects	534:544	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads	534:782	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	0	13	theme	dyes	110:113	arg1	removal					78:84	the removal	74:84	the removal of anionic and cationic dyes	74:113	Mesoporous crosslinked chitosan-activated clinoptilolite biocomposite for the removal of anionic and cationic dyes.					
35598510	8	14	theme	spontaneous	1447:1457	arg1	adsorption					1408:1417	the adsorption	1404:1417	the adsorption of dyes on the CS-GA/ACP	1404:1442	The CS-GA/ACP can be recycled to remove dyes without significant loss of efficacy, and the adsorption of dyes on the CS-GA/ACP is spontaneous endothermic adsorption.					
35598510	8	14	theme	spontaneous	1447:1457	arg1	adsorption					1471:1480	spontaneous endothermic adsorption	1447:1480	spontaneous endothermic adsorption	1447:1480	The CS-GA/ACP can be recycled to remove dyes without significant loss of efficacy, and the adsorption of dyes on the CS-GA/ACP is spontaneous endothermic adsorption.					
35598510	4	15	theme	CS-GA/ACP	758:766	arg1	beads					778:782	the CS-GA/ACP composite beads	754:782	the CS-GA/ACP composite beads	754:782	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	7	16	theme	possible	1160:1167	arg1	H-bonding					1259:1267	H-bonding	1259:1267	H-bonding	1259:1267	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	16	theme	possible	1160:1167	arg1	interaction					1304:1314	n-π interaction	1300:1314	n-π interaction	1300:1314	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	16	theme	possible	1160:1167	arg1	interaction					1169:1179	the possible interaction	1156:1179	the possible interaction between the adsorbent and dye molecules	1156:1219	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	16	theme	possible	1160:1167	arg1	Yoshida					1226:1232	Yoshida	1226:1232	Yoshida	1226:1232	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	16	theme	possible	1160:1167	arg1	H-bonding					1234:1242	H-bonding	1234:1242	H-bonding	1234:1242	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	16	theme	possible	1160:1167	arg1	interaction					1284:1294	electrostatic interaction	1270:1294	electrostatic interaction	1270:1294	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	4	17	theme	contact	651:657	arg1	5-120 min					665:673	5-120 min	665:673	5-120 min	665:673	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	17	theme	contact	651:657	arg1	time					659:662	contact time	651:662	contact time (5-120 min)	651:674	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	17	theme	contact	651:657	arg1	dosages					571:577	CS-GA/ACP dosages	561:577	CS-GA/ACP dosages (0.005-0.25 g)	561:592	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	18	theme	CS	549:550	arg1	loading					552:558	CS loading	549:558	CS loading	549:558	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	18	theme	CS	549:550	arg1	dosages					571:577	CS-GA/ACP dosages	561:577	CS-GA/ACP dosages (0.005-0.25 g)	561:592	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	19	theme	CR	741:742	arg1	adsorption					727:736	the adsorption	723:736	the adsorption of CR and MB on the CS-GA/ACP composite beads	723:782	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	3	20	theme	CS-GA/ACP	437:445	arg1	properties					419:428	The adsorption properties	404:428	The adsorption properties of the CS-GA/ACP for Congo red (CR) and methylene blue (MB) removal	404:496	The adsorption properties of the CS-GA/ACP for Congo red (CR) and methylene blue (MB) removal were examined using a batch method.					
35598510	4	21	from	effects	538:544	arg1	adsorption					727:736	the adsorption	723:736	the adsorption of CR and MB on the CS-GA/ACP composite beads	723:782	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	5	22	theme	pseudo-first-order	890:907	arg1	kinetics					909:916	the pseudo-first-order kinetics	886:916	the pseudo-first-order kinetics	886:916	The pseudo-second-order kinetics could better describe the adsorption process than the pseudo-first-order kinetics, and the Langmuir isotherms model agreed well with the experimental data.					
35598510	2	23	theme	BET	388:390	arg1	techniques					392:401	FTIR, SEM, and BET techniques	373:401	FTIR, SEM, and BET techniques	373:401	Structural analysis of the CS-GA/ACP composite beads was performed using FTIR, SEM, and BET techniques.					
35598510	8	24	theme	endothermic	1459:1469	arg1	adsorption					1408:1417	the adsorption	1404:1417	the adsorption of dyes on the CS-GA/ACP	1404:1442	The CS-GA/ACP can be recycled to remove dyes without significant loss of efficacy, and the adsorption of dyes on the CS-GA/ACP is spontaneous endothermic adsorption.					
35598510	8	24	theme	endothermic	1459:1469	arg1	adsorption					1471:1480	spontaneous endothermic adsorption	1447:1480	spontaneous endothermic adsorption	1447:1480	The CS-GA/ACP can be recycled to remove dyes without significant loss of efficacy, and the adsorption of dyes on the CS-GA/ACP is spontaneous endothermic adsorption.					
35598510	4	25	theme	MB	748:749	arg1	adsorption					727:736	the adsorption	723:736	the adsorption of CR and MB on the CS-GA/ACP composite beads	723:782	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	2	26	theme	CS-GA/ACP	327:335	arg1	beads					347:351	the CS-GA/ACP composite beads	323:351	the CS-GA/ACP composite beads	323:351	Structural analysis of the CS-GA/ACP composite beads was performed using FTIR, SEM, and BET techniques.					
35598510	6	27	from	25 ℃	1093:1096	arg1	180.59 mg/g					1062:1072	180.59 mg/g	1062:1072	180.59 mg/g	1062:1072	The maximum adsorption capacities of the CS-GA/ACP for CR and MB were 180.59 mg/g and 143.67 mg/g at 25 ℃, respectively.					
35598510	6	27	from	25 ℃	1093:1096	arg1	capacities					1015:1024	The maximum adsorption capacities	992:1024	The maximum adsorption capacities of the CS-GA/ACP for CR and MB	992:1055	The maximum adsorption capacities of the CS-GA/ACP for CR and MB were 180.59 mg/g and 143.67 mg/g at 25 ℃, respectively.					
35598510	6	27	from	25 ℃	1093:1096	arg1	143.67 mg/g					1078:1088	143.67 mg/g	1078:1088	143.67 mg/g	1078:1088	The maximum adsorption capacities of the CS-GA/ACP for CR and MB were 180.59 mg/g and 143.67 mg/g at 25 ℃, respectively.					
35598510	1	28	theme	crosslinking	280:291	arg1	agent					293:297	the crosslinking agent	276:297	the crosslinking agent	276:297	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	9	29	theme	aqueous	1558:1564	arg1	solution					1566:1573	aqueous solution	1558:1573	aqueous solution	1558:1573	Overall, the CS-GA/ACP showed an excellent performance for dyes removal in aqueous solution and could be a practical candidate for industrial applications.					
35598510	4	30	theme	ionic	677:681	arg1	dosages					571:577	CS-GA/ACP dosages	561:577	CS-GA/ACP dosages (0.005-0.25 g)	561:592	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	30	theme	ionic	677:681	arg1	strength					683:690	ionic strength	677:690	ionic strength	677:690	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	0	31	theme	clinoptilolite	42:55	arg1	biocomposite					57:68	chitosan-activated clinoptilolite biocomposite	23:68	chitosan-activated clinoptilolite biocomposite	23:68	Mesoporous crosslinked chitosan-activated clinoptilolite biocomposite for the removal of anionic and cationic dyes.					
35598510	8	32	theme	significant	1370:1380	arg1	loss					1382:1385	significant loss	1370:1385	significant loss of efficacy	1370:1397	The CS-GA/ACP can be recycled to remove dyes without significant loss of efficacy, and the adsorption of dyes on the CS-GA/ACP is spontaneous endothermic adsorption.					
35598510	3	33	theme	batch	520:524	arg1	method					526:531	a batch method	518:531	a batch method	518:531	The adsorption properties of the CS-GA/ACP for Congo red (CR) and methylene blue (MB) removal were examined using a batch method.					
35598510	0	34	theme	chitosan-activated	23:40	arg1	biocomposite					57:68	chitosan-activated clinoptilolite biocomposite	23:68	chitosan-activated clinoptilolite biocomposite	23:68	Mesoporous crosslinked chitosan-activated clinoptilolite biocomposite for the removal of anionic and cationic dyes.					
35598510	1	35	theme	mesoporous	118:127	arg1	glutaraldehyde					253:266	glutaraldehyde	253:266	glutaraldehyde (GA)	253:271	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	35	theme	mesoporous	118:127	arg1	CS-GA/ACP					189:197	CS-GA/ACP	189:197	CS-GA/ACP	189:197	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	35	theme	mesoporous	118:127	arg1	substrate					239:247	substrate	239:247	substrate	239:247	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	35	theme	mesoporous	118:127	arg1	biocomposite					175:186	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite	116:186	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP)	116:198	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	9	36	from	performance	1526:1536	arg1	solution					1566:1573	aqueous solution	1558:1573	aqueous solution	1558:1573	Overall, the CS-GA/ACP showed an excellent performance for dyes removal in aqueous solution and could be a practical candidate for industrial applications.					
35598510	4	37	theme	initial	613:619	arg1	concentrations					621:634	initial concentrations	613:634	initial concentrations (30-300 mg/L)	613:648	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	37	theme	initial	613:619	arg1	dosages					571:577	CS-GA/ACP dosages	561:577	CS-GA/ACP dosages (0.005-0.25 g)	561:592	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	37	theme	initial	613:619	arg1	30-300 mg/L					637:647	30-300 mg/L	637:647	30-300 mg/L	637:647	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	3	38	theme	adsorption	408:417	arg1	properties					419:428	The adsorption properties	404:428	The adsorption properties of the CS-GA/ACP for Congo red (CR) and methylene blue (MB) removal	404:496	The adsorption properties of the CS-GA/ACP for Congo red (CR) and methylene blue (MB) removal were examined using a batch method.					
35598510	3	39	theme	blue	480:483	arg1	methylene					470:478	methylene blue	470:483	methylene blue (MB)	470:488	The adsorption properties of the CS-GA/ACP for Congo red (CR) and methylene blue (MB) removal were examined using a batch method.					
35598510	3	39	theme	blue	480:483	arg1	MB					486:487	MB	486:487	MB	486:487	The adsorption properties of the CS-GA/ACP for Congo red (CR) and methylene blue (MB) removal were examined using a batch method.					
35598510	5	40	theme	Langmuir	927:934	arg1	model					946:950	the Langmuir isotherms model	923:950	the Langmuir isotherms model	923:950	The pseudo-second-order kinetics could better describe the adsorption process than the pseudo-first-order kinetics, and the Langmuir isotherms model agreed well with the experimental data.					
35598510	4	41	theme	pH	595:596	arg1	dosages					571:577	CS-GA/ACP dosages	561:577	CS-GA/ACP dosages (0.005-0.25 g)	561:592	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	41	theme	pH	595:596	arg1	3-11					606:609	3-11	606:609	3-11	606:609	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	41	theme	pH	595:596	arg1	values					598:603	pH values	595:603	pH values (3-11)	595:610	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	6	42	theme	maximum	996:1002	arg1	180.59 mg/g					1062:1072	180.59 mg/g	1062:1072	180.59 mg/g	1062:1072	The maximum adsorption capacities of the CS-GA/ACP for CR and MB were 180.59 mg/g and 143.67 mg/g at 25 ℃, respectively.					
35598510	6	42	theme	maximum	996:1002	arg1	capacities					1015:1024	The maximum adsorption capacities	992:1024	The maximum adsorption capacities of the CS-GA/ACP for CR and MB	992:1055	The maximum adsorption capacities of the CS-GA/ACP for CR and MB were 180.59 mg/g and 143.67 mg/g at 25 ℃, respectively.					
35598510	1	43	dep	substrate	239:247	arg1	the					235:237	the	235:237	the	235:237	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	5	44	theme	isotherms	936:944	arg1	model					946:950	the Langmuir isotherms model	923:950	the Langmuir isotherms model	923:950	The pseudo-second-order kinetics could better describe the adsorption process than the pseudo-first-order kinetics, and the Langmuir isotherms model agreed well with the experimental data.					
35598510	4	45	from	adsorption	727:736	arg1	beads					778:782	the CS-GA/ACP composite beads	754:782	the CS-GA/ACP composite beads	754:782	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	8	46	theme	efficacy	1390:1397	arg1	loss					1382:1385	significant loss	1370:1385	significant loss of efficacy	1370:1397	The CS-GA/ACP can be recycled to remove dyes without significant loss of efficacy, and the adsorption of dyes on the CS-GA/ACP is spontaneous endothermic adsorption.					
35598510	8	47	theme	dyes	1422:1425	arg1	adsorption					1408:1417	the adsorption	1404:1417	the adsorption of dyes on the CS-GA/ACP	1404:1442	The CS-GA/ACP can be recycled to remove dyes without significant loss of efficacy, and the adsorption of dyes on the CS-GA/ACP is spontaneous endothermic adsorption.					
35598510	8	47	theme	dyes	1422:1425	arg1	adsorption					1471:1480	spontaneous endothermic adsorption	1447:1480	spontaneous endothermic adsorption	1447:1480	The CS-GA/ACP can be recycled to remove dyes without significant loss of efficacy, and the adsorption of dyes on the CS-GA/ACP is spontaneous endothermic adsorption.					
35598510	1	48	theme	crosslinked	129:139	arg1	glutaraldehyde					253:266	glutaraldehyde	253:266	glutaraldehyde (GA)	253:271	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	48	theme	crosslinked	129:139	arg1	CS-GA/ACP					189:197	CS-GA/ACP	189:197	CS-GA/ACP	189:197	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	48	theme	crosslinked	129:139	arg1	substrate					239:247	substrate	239:247	substrate	239:247	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	48	theme	crosslinked	129:139	arg1	biocomposite					175:186	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite	116:186	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP)	116:198	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	9	49	theme	practical	1590:1598	arg1	candidate					1600:1608	a practical candidate	1588:1608	a practical candidate for industrial applications	1588:1636	Overall, the CS-GA/ACP showed an excellent performance for dyes removal in aqueous solution and could be a practical candidate for industrial applications.					
35598510	1	50	theme	chitosan-activated	141:158	arg1	glutaraldehyde					253:266	glutaraldehyde	253:266	glutaraldehyde (GA)	253:271	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	50	theme	chitosan-activated	141:158	arg1	CS-GA/ACP					189:197	CS-GA/ACP	189:197	CS-GA/ACP	189:197	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	50	theme	chitosan-activated	141:158	arg1	substrate					239:247	substrate	239:247	substrate	239:247	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	50	theme	chitosan-activated	141:158	arg1	biocomposite					175:186	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite	116:186	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP)	116:198	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	7	51	dep	H-bonding	1234:1242	arg1	H-bonding					1259:1267	H-bonding	1259:1267	H-bonding	1259:1267	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	51	dep	H-bonding	1234:1242	arg1	interaction					1304:1314	n-π interaction	1300:1314	n-π interaction	1300:1314	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	51	dep	H-bonding	1234:1242	arg1	interaction					1169:1179	the possible interaction	1156:1179	the possible interaction between the adsorbent and dye molecules	1156:1219	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	51	dep	H-bonding	1234:1242	arg1	Yoshida					1226:1232	Yoshida	1226:1232	Yoshida	1226:1232	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	51	dep	H-bonding	1234:1242	arg1	H-bonding					1234:1242	H-bonding	1234:1242	H-bonding	1234:1242	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	51	dep	H-bonding	1234:1242	arg1	interaction					1284:1294	electrostatic interaction	1270:1294	electrostatic interaction	1270:1294	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	1	52	link	crosslinked	129:139	arg1	glutaraldehyde					253:266	glutaraldehyde	253:266	glutaraldehyde (GA)	253:271	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	52	link	crosslinked	129:139	arg1	CS-GA/ACP					189:197	CS-GA/ACP	189:197	CS-GA/ACP	189:197	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	52	link	crosslinked	129:139	arg1	substrate					239:247	substrate	239:247	substrate	239:247	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	52	link	crosslinked	129:139	arg1	biocomposite					175:186	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite	116:186	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP)	116:198	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	53	theme	clinoptilolite	160:173	arg1	glutaraldehyde					253:266	glutaraldehyde	253:266	glutaraldehyde (GA)	253:271	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	53	theme	clinoptilolite	160:173	arg1	CS-GA/ACP					189:197	CS-GA/ACP	189:197	CS-GA/ACP	189:197	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	53	theme	clinoptilolite	160:173	arg1	substrate					239:247	substrate	239:247	substrate	239:247	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	1	53	theme	clinoptilolite	160:173	arg1	biocomposite					175:186	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite	116:186	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP)	116:198	A mesoporous crosslinked chitosan-activated clinoptilolite biocomposite (CS-GA/ACP) was prepared with chitosan (CS) as the substrate and glutaraldehyde (GA) as the crosslinking agent.					
35598510	6	54	theme	CS-GA/ACP	1033:1041	arg1	180.59 mg/g					1062:1072	180.59 mg/g	1062:1072	180.59 mg/g	1062:1072	The maximum adsorption capacities of the CS-GA/ACP for CR and MB were 180.59 mg/g and 143.67 mg/g at 25 ℃, respectively.					
35598510	6	54	theme	CS-GA/ACP	1033:1041	arg1	capacities					1015:1024	The maximum adsorption capacities	992:1024	The maximum adsorption capacities of the CS-GA/ACP for CR and MB	992:1055	The maximum adsorption capacities of the CS-GA/ACP for CR and MB were 180.59 mg/g and 143.67 mg/g at 25 ℃, respectively.					
35598510	4	55	theme	CS-GA/ACP	561:569	arg1	0.005-0.25 g					580:591	0.005-0.25 g	580:591	0.005-0.25 g	580:591	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	55	theme	CS-GA/ACP	561:569	arg1	loading					552:558	CS loading	549:558	CS loading	549:558	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	55	theme	CS-GA/ACP	561:569	arg1	strength					683:690	ionic strength	677:690	ionic strength	677:690	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	55	theme	CS-GA/ACP	561:569	arg1	concentrations					621:634	initial concentrations	613:634	initial concentrations (30-300 mg/L)	613:648	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	55	theme	CS-GA/ACP	561:569	arg1	time					659:662	contact time	651:662	contact time (5-120 min)	651:674	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	55	theme	CS-GA/ACP	561:569	arg1	dosages					571:577	CS-GA/ACP dosages	561:577	CS-GA/ACP dosages (0.005-0.25 g)	561:592	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	55	theme	CS-GA/ACP	561:569	arg1	values					598:603	pH values	595:603	pH values (3-11)	595:610	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	4	55	theme	CS-GA/ACP	561:569	arg1	temperatures					697:708	temperatures	697:708	temperatures (25-65 ℃)	697:718	The effects of CS loading, CS-GA/ACP dosages (0.005-0.25 g), pH values (3-11), initial concentrations (30-300 mg/L), contact time (5-120 min), ionic strength, and temperatures (25-65 ℃) on the adsorption of CR and MB on the CS-GA/ACP composite beads were investigated.					
35598510	3	56	dep	red	457:459	arg1	removal					490:496	removal	490:496	removal	490:496	The adsorption properties of the CS-GA/ACP for Congo red (CR) and methylene blue (MB) removal were examined using a batch method.					
35598510	2	57	theme	composite	337:345	arg1	beads					347:351	the CS-GA/ACP composite beads	323:351	the CS-GA/ACP composite beads	323:351	Structural analysis of the CS-GA/ACP composite beads was performed using FTIR, SEM, and BET techniques.					
35598510	8	58	from	adsorption	1408:1417	arg1	CS-GA/ACP					1434:1442	the CS-GA/ACP	1430:1442	the CS-GA/ACP	1430:1442	The CS-GA/ACP can be recycled to remove dyes without significant loss of efficacy, and the adsorption of dyes on the CS-GA/ACP is spontaneous endothermic adsorption.					
35598510	2	59	theme	beads	347:351	arg1	analysis					311:318	Structural analysis	300:318	Structural analysis of the CS-GA/ACP composite beads	300:351	Structural analysis of the CS-GA/ACP composite beads was performed using FTIR, SEM, and BET techniques.					
35598510	0	60	theme	anionic	89:95	arg1	dyes					110:113	anionic and cationic dyes	89:113	anionic and cationic dyes	89:113	Mesoporous crosslinked chitosan-activated clinoptilolite biocomposite for the removal of anionic and cationic dyes.					
35598510	5	61	theme	experimental	973:984	arg1	data					986:989	the experimental data	969:989	the experimental data	969:989	The pseudo-second-order kinetics could better describe the adsorption process than the pseudo-first-order kinetics, and the Langmuir isotherms model agreed well with the experimental data.					
35598510	7	62	theme	electrostatic	1270:1282	arg1	H-bonding					1259:1267	H-bonding	1259:1267	H-bonding	1259:1267	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	62	theme	electrostatic	1270:1282	arg1	interaction					1304:1314	n-π interaction	1300:1314	n-π interaction	1300:1314	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	62	theme	electrostatic	1270:1282	arg1	interaction					1169:1179	the possible interaction	1156:1179	the possible interaction between the adsorbent and dye molecules	1156:1219	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	62	theme	electrostatic	1270:1282	arg1	Yoshida					1226:1232	Yoshida	1226:1232	Yoshida	1226:1232	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	62	theme	electrostatic	1270:1282	arg1	H-bonding					1234:1242	H-bonding	1234:1242	H-bonding	1234:1242	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	62	theme	electrostatic	1270:1282	arg1	interaction					1284:1294	electrostatic interaction	1270:1294	electrostatic interaction	1270:1294	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	2	63	theme	SEM	379:381	arg1	techniques					392:401	FTIR, SEM, and BET techniques	373:401	FTIR, SEM, and BET techniques	373:401	Structural analysis of the CS-GA/ACP composite beads was performed using FTIR, SEM, and BET techniques.					
35598510	9	64	theme	excellent	1516:1524	arg1	performance					1526:1536	an excellent performance	1513:1536	an excellent performance for dyes removal in aqueous solution	1513:1573	Overall, the CS-GA/ACP showed an excellent performance for dyes removal in aqueous solution and could be a practical candidate for industrial applications.					
35598510	7	65	theme	dye	1207:1209	arg1	molecules					1211:1219	the adsorbent and dye molecules	1189:1219	molecules	1211:1219	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35598510	7	66	theme	proposed	1117:1124	arg1	mechanism					1126:1134	The proposed mechanism	1113:1134	The proposed mechanism studies	1113:1142	The proposed mechanism studies showed that the possible interaction between the adsorbent and dye molecules were Yoshida H-bonding, dipole-dipole H-bonding, electrostatic interaction and n-π interaction.					
35578339	4	0	theme	UC	892:893	arg1	model					901:905	a dextran sulfate sodium (DSS)-induced UC mouse model	853:905	a dextran sulfate sodium (DSS)-induced UC mouse model	853:905	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	8	1	theme	EVs	1795:1797	arg1	efficacy					1783:1790	the potential efficacy	1769:1790	the potential efficacy of EVs from commensal and probiotic Clostridium species against UC	1769:1857	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	7	2	theme	EVs	1530:1532	arg1	impact					1520:1525	the impact	1516:1525	the impact of EVs on the reprogramming of the M2 macrophages	1516:1575	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	4	3	theme	western	687:693	arg1	blot					695:698	western blot	687:698	western blot	687:698	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	3	4	theme	potential	554:562	arg1	role					564:567	the potential role	550:567	the potential role of C. butyricum-secreted EVs in commensals-host crosstalk	550:625	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	8	5	theme	commensal	1804:1812	arg1	species					1840:1846	commensal and probiotic Clostridium species	1804:1846	commensal and probiotic Clostridium species	1804:1846	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	8	6	theme	probiotic	1818:1826	arg1	species					1840:1846	commensal and probiotic Clostridium species	1804:1846	commensal and probiotic Clostridium species	1804:1846	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	6	7	theme	macrophages	1206:1216	arg1	transformation					1188:1201	the transformation	1184:1201	the transformation of macrophages to the M2 type	1184:1231	Our findings showed that C. butyricum-derived EVs improved the remission of murine colitis and polarized the transformation of macrophages to the M2 type.					
35578339	3	8	theme	butyricum-secreted	575:592	arg1	EVs					594:596	C. butyricum-secreted EVs	572:596	C. butyricum-secreted EVs	572:596	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	7	9	theme	Bacteroides	1400:1410	arg1	abundance					1320:1328	the relative abundance	1307:1328	the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages	1307:1575	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	1	10	theme	cellular	249:256	arg1	communication					258:270	cellular communication	249:270	cellular communication	249:270	BACKGROUND The extracellular vesicles (EVs) traffic constitutes an essential pathway of cellular communication.					
35578339	7	11	theme	faecal	1429:1434	arg1	transplantations					1436:1451	faecal transplantations	1429:1451	faecal transplantations from EVs-treated mice	1429:1473	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	6	12	theme	colitis	1162:1168	arg1	remission					1142:1150	the remission	1138:1150	the remission of murine colitis	1138:1168	Our findings showed that C. butyricum-derived EVs improved the remission of murine colitis and polarized the transformation of macrophages to the M2 type.					
35578339	7	13	from	mice	1470:1473	arg1	transplantations					1436:1451	faecal transplantations	1429:1451	faecal transplantations from EVs-treated mice	1429:1473	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	3	14	used	used	507:510	arg2	butyricum					470:478	Clostridium butyricum	458:478	Clostridium butyricum (C. butyricum)	458:493	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	3	14	used	used	507:510	arg2	butyricum					484:492	C. butyricum	481:492	C. butyricum	481:492	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	4	15	dep	RESULTS	644:650	arg1	performed					661:669	performed	661:669	performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model	661:905	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	5	16	theme	faecal	965:970	arg1	transplantations					972:987	faecal transplantations	965:987	faecal transplantations	965:987	The antibiotic cocktail-induced microbiome depletion and faecal transplantations were used to further investigate the mechanisms by which EVs regulate macrophage balance.					
35578339	4	17	theme	rRNA	730:733	arg1	analysis					735:742	16S rRNA analysis	726:742	16S rRNA analysis	726:742	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	7	18	theme	gut	1281:1283	arg1	dysbiosis					1285:1293	gut dysbiosis	1281:1293	gut dysbiosis	1281:1293	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	5	19	theme	cocktail-induced	923:938	arg1	depletion					951:959	The antibiotic cocktail-induced microbiome depletion	908:959	The antibiotic cocktail-induced microbiome depletion	908:959	The antibiotic cocktail-induced microbiome depletion and faecal transplantations were used to further investigate the mechanisms by which EVs regulate macrophage balance.					
35578339	1	20	dep	BACKGROUND	161:170	arg1	traffic					205:211	traffic	205:211	traffic constitutes an essential pathway of cellular communication	205:270	BACKGROUND The extracellular vesicles (EVs) traffic constitutes an essential pathway of cellular communication.					
35578339	8	21	theme	CONCLUSION	1578:1587	arg1	EVs					1610:1612	CONCLUSION C. butyricum-derived EVs	1578:1612	CONCLUSION C. butyricum-derived EVs	1578:1612	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	3	22	theme	C.	481:482	arg1	butyricum					470:478	Clostridium butyricum	458:478	Clostridium butyricum (C. butyricum)	458:493	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	3	22	theme	C.	481:482	arg1	butyricum					484:492	C. butyricum	481:492	C. butyricum	481:492	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	7	23	theme	C.	1247:1248	arg1	EVs					1268:1270	C. butyricum-derived EVs	1247:1270	C. butyricum-derived EVs	1247:1270	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	8	24	theme	butyricum-derived	1592:1608	arg1	EVs					1610:1612	CONCLUSION C. butyricum-derived EVs	1578:1612	CONCLUSION C. butyricum-derived EVs	1578:1612	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	8	25	from	efficacy	1783:1790	arg1	species					1840:1846	commensal and probiotic Clostridium species	1804:1846	commensal and probiotic Clostridium species	1804:1846	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	0	26	theme	Commensal	0:8	arg1	vesicles					41:48	Commensal bacteria-derived extracellular vesicles	0:48	Commensal bacteria-derived extracellular vesicles	0:48	Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota.					
35578339	0	27	dep	macrophages	101:111	arg1	macrophages					101:111	the macrophages polarization and remodeling	97:139	the macrophages polarization and remodeling the gut microbiota	97:158	Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota.					
35578339	0	27	dep	macrophages	101:111	arg1	remodeling					130:139	remodeling	130:139	remodeling	130:139	Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota.					
35578339	0	27	dep	macrophages	101:111	arg1	microbiota					149:158	the gut microbiota	141:158	the macrophages polarization and remodeling the gut microbiota	97:158	Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota.					
35578339	0	27	dep	macrophages	101:111	arg1	polarization					113:124	polarization	113:124	polarization	113:124	Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota.					
35578339	6	28	theme	butyricum-derived	1107:1123	arg1	EVs					1125:1127	C. butyricum-derived EVs	1104:1127	C. butyricum-derived EVs	1104:1127	Our findings showed that C. butyricum-derived EVs improved the remission of murine colitis and polarized the transformation of macrophages to the M2 type.					
35578339	4	29	theme	butyricum-derived	770:786	arg1	EVs					788:790	C. butyricum-derived EVs	767:790	C. butyricum-derived EVs	767:790	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	0	30	theme	extracellular	27:39	arg1	vesicles					41:48	Commensal bacteria-derived extracellular vesicles	0:48	Commensal bacteria-derived extracellular vesicles	0:48	Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota.					
35578339	7	31	theme	Akkermansia	1384:1394	arg1	abundance					1320:1328	the relative abundance	1307:1328	the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages	1307:1575	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	3	32	theme	ulcerative	525:534	arg1	UC					545:546	UC	545:546	UC	545:546	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	3	32	theme	ulcerative	525:534	arg1	colitis					536:542	ulcerative colitis	525:542	ulcerative colitis (UC)	525:547	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	7	33	theme	macrophages	1565:1575	arg1	reprogramming					1541:1553	the reprogramming	1537:1553	the reprogramming of the M2 macrophages	1537:1575	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	7	34	theme	Escherichia-Shigella	1347:1366	arg1	abundance					1320:1328	the relative abundance	1307:1328	the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages	1307:1575	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	8	35	from	species	1840:1846	arg1	EVs					1795:1797	EVs	1795:1797	EVs from commensal and probiotic Clostridium species	1795:1846	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	8	35	from	species	1840:1846	arg1	efficacy					1783:1790	the potential efficacy	1769:1790	the potential efficacy of EVs from commensal and probiotic Clostridium species against UC	1769:1857	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	1	36	theme	communication	258:270	arg1	pathway					238:244	an essential pathway	225:244	an essential pathway of cellular communication	225:270	BACKGROUND The extracellular vesicles (EVs) traffic constitutes an essential pathway of cellular communication.					
35578339	7	37	theme	UC	1500:1501	arg1	symptoms					1488:1495	the symptoms	1484:1495	the symptoms of UC	1484:1501	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	6	38	theme	murine	1155:1160	arg1	colitis					1162:1168	murine colitis	1155:1168	murine colitis	1155:1168	Our findings showed that C. butyricum-derived EVs improved the remission of murine colitis and polarized the transformation of macrophages to the M2 type.					
35578339	8	39	theme	microbiota	1746:1755	arg1	composition					1727:1737	the composition	1723:1737	the composition of gut microbiota	1723:1755	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	8	40	link	butyricum-derived	1592:1608	arg1	EVs					1610:1612	CONCLUSION C. butyricum-derived EVs	1578:1612	CONCLUSION C. butyricum-derived EVs	1578:1612	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	8	41	theme	M2	1692:1693	arg1	macrophages					1695:1705	M2 macrophages	1692:1705	M2 macrophages	1692:1705	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	6	42	link	butyricum-derived	1107:1123	arg1	EVs					1125:1127	C. butyricum-derived EVs	1104:1127	C. butyricum-derived EVs	1104:1127	Our findings showed that C. butyricum-derived EVs improved the remission of murine colitis and polarized the transformation of macrophages to the M2 type.					
35578339	4	43	theme	-induced	883:890	arg1	model					901:905	a dextran sulfate sodium (DSS)-induced UC mouse model	853:905	a dextran sulfate sodium (DSS)-induced UC mouse model	853:905	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	8	44	theme	potential	1773:1781	arg1	efficacy					1783:1790	the potential efficacy	1769:1790	the potential efficacy of EVs from commensal and probiotic Clostridium species against UC	1769:1857	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	4	45	theme	mouse	895:899	arg1	model					901:905	a dextran sulfate sodium (DSS)-induced UC mouse model	853:905	a dextran sulfate sodium (DSS)-induced UC mouse model	853:905	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	0	46	link	bacteria-derived	10:25	arg1	vesicles					41:48	Commensal bacteria-derived extracellular vesicles	0:48	Commensal bacteria-derived extracellular vesicles	0:48	Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota.					
35578339	7	47	link	butyricum-derived	1250:1266	arg1	EVs					1268:1270	C. butyricum-derived EVs	1247:1270	C. butyricum-derived EVs	1247:1270	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	6	48	theme	M2	1225:1226	arg1	type					1228:1231	the M2 type	1221:1231	the M2 type	1221:1231	Our findings showed that C. butyricum-derived EVs improved the remission of murine colitis and polarized the transformation of macrophages to the M2 type.					
35578339	3	49	theme	EVs	594:596	arg1	role					564:567	the potential role	550:567	the potential role of C. butyricum-secreted EVs in commensals-host crosstalk	550:625	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	7	50	from	impact	1520:1525	arg1	reprogramming					1541:1553	the reprogramming	1537:1553	the reprogramming of the M2 macrophages	1537:1575	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	3	51	theme	C.	572:573	arg1	EVs					594:596	C. butyricum-secreted EVs	572:596	C. butyricum-secreted EVs	572:596	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	1	52	theme	essential	228:236	arg1	pathway					238:244	an essential pathway	225:244	an essential pathway of cellular communication	225:270	BACKGROUND The extracellular vesicles (EVs) traffic constitutes an essential pathway of cellular communication.					
35578339	8	53	theme	Clostridium	1828:1838	arg1	species					1840:1846	commensal and probiotic Clostridium species	1804:1846	commensal and probiotic Clostridium species	1804:1846	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	7	54	theme	EVs-treated	1458:1468	arg1	mice					1470:1473	EVs-treated mice	1458:1473	EVs-treated mice	1458:1473	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	4	55	theme	flow	671:674	arg1	cytometry					676:684	flow cytometry	671:684	flow cytometry	671:684	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	3	56	theme	commensals-host	601:615	arg1	crosstalk					617:625	commensals-host crosstalk	601:625	commensals-host crosstalk	601:625	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	5	57	theme	microbiome	940:949	arg1	depletion					951:959	The antibiotic cocktail-induced microbiome depletion	908:959	The antibiotic cocktail-induced microbiome depletion	908:959	The antibiotic cocktail-induced microbiome depletion and faecal transplantations were used to further investigate the mechanisms by which EVs regulate macrophage balance.					
35578339	4	58	theme	gut	823:825	arg1	composition					838:848	gut microbiota composition	823:848	gut microbiota composition	823:848	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	1	59	theme	extracellular	176:188	arg1	vesicles					190:197	The extracellular vesicles	172:197	The extracellular vesicles (EVs)	172:203	BACKGROUND The extracellular vesicles (EVs) traffic constitutes an essential pathway of cellular communication.					
35578339	1	59	theme	extracellular	176:188	arg1	EVs					200:202	EVs	200:202	EVs	200:202	BACKGROUND The extracellular vesicles (EVs) traffic constitutes an essential pathway of cellular communication.					
35578339	5	60	theme	antibiotic	912:921	arg1	depletion					951:959	The antibiotic cocktail-induced microbiome depletion	908:959	The antibiotic cocktail-induced microbiome depletion	908:959	The antibiotic cocktail-induced microbiome depletion and faecal transplantations were used to further investigate the mechanisms by which EVs regulate macrophage balance.					
35578339	4	61	theme	16S	726:728	arg1	analysis					735:742	16S rRNA analysis	726:742	16S rRNA analysis	726:742	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	3	62	from	role	564:567	arg1	crosstalk					617:625	commensals-host crosstalk	601:625	commensals-host crosstalk	601:625	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	7	63	theme	butyricum-derived	1250:1266	arg1	EVs					1268:1270	C. butyricum-derived EVs	1247:1270	C. butyricum-derived EVs	1247:1270	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	8	64	theme	C.	1589:1590	arg1	EVs					1610:1612	CONCLUSION C. butyricum-derived EVs	1578:1612	CONCLUSION C. butyricum-derived EVs	1578:1612	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	4	65	theme	EVs	788:790	arg1	role					759:762	the role	755:762	the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition	755:848	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	0	66	theme	gut	145:147	arg1	microbiota					149:158	the gut microbiota	141:158	the macrophages polarization and remodeling the gut microbiota	97:158	Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota.					
35578339	4	67	theme	macrophage	795:804	arg1	polarization					806:817	macrophage polarization	795:817	macrophage polarization	795:817	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	5	68	used	used	994:997	arg2	transplantations					972:987	faecal transplantations	965:987	faecal transplantations	965:987	The antibiotic cocktail-induced microbiome depletion and faecal transplantations were used to further investigate the mechanisms by which EVs regulate macrophage balance.					
35578339	5	68	used	used	994:997	arg2	depletion					951:959	The antibiotic cocktail-induced microbiome depletion	908:959	The antibiotic cocktail-induced microbiome depletion	908:959	The antibiotic cocktail-induced microbiome depletion and faecal transplantations were used to further investigate the mechanisms by which EVs regulate macrophage balance.					
35578339	0	69	theme	bacteria-derived	10:25	arg1	vesicles					41:48	Commensal bacteria-derived extracellular vesicles	0:48	Commensal bacteria-derived extracellular vesicles	0:48	Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota.					
35578339	2	70	theme	immune	421:426	arg1	system					428:433	the immune system	417:433	the immune system	417:433	And the molecules in EVs produced by procaryotes help in maintaining homeostasis, addressing microbial imbalance and infections, and regulating the immune system.					
35578339	4	71	from	role	759:762	arg1	composition					838:848	gut microbiota composition	823:848	gut microbiota composition	823:848	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	4	71	from	role	759:762	arg1	polarization					806:817	macrophage polarization	795:817	macrophage polarization	795:817	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	2	72	from	molecules	281:289	arg1	EVs					294:296	EVs	294:296	EVs produced by procaryotes	294:320	And the molecules in EVs produced by procaryotes help in maintaining homeostasis, addressing microbial imbalance and infections, and regulating the immune system.					
35578339	6	73	theme	C.	1104:1105	arg1	EVs					1125:1127	C. butyricum-derived EVs	1104:1127	C. butyricum-derived EVs	1104:1127	Our findings showed that C. butyricum-derived EVs improved the remission of murine colitis and polarized the transformation of macrophages to the M2 type.					
35578339	8	74	theme	DSS-induced	1636:1646	arg1	colitis					1648:1654	DSS-induced colitis	1636:1654	DSS-induced colitis	1636:1654	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	4	75	theme	C.	767:768	arg1	EVs					788:790	C. butyricum-derived EVs	767:790	C. butyricum-derived EVs	767:790	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	7	76	theme	relative	1311:1318	arg1	abundance					1320:1328	the relative abundance	1307:1328	the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages	1307:1575	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	0	77	theme	ulcerative	59:68	arg1	colitis					70:76	ulcerative colitis	59:76	ulcerative colitis	59:76	Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota.					
35578339	5	78	theme	macrophage	1059:1068	arg1	balance					1070:1076	macrophage balance	1059:1076	macrophage balance	1059:1076	The antibiotic cocktail-induced microbiome depletion and faecal transplantations were used to further investigate the mechanisms by which EVs regulate macrophage balance.					
35578339	3	79	theme	Clostridium	458:468	arg1	butyricum					470:478	Clostridium butyricum	458:478	Clostridium butyricum (C. butyricum)	458:493	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	3	79	theme	Clostridium	458:468	arg1	butyricum					484:492	C. butyricum	481:492	C. butyricum	481:492	Despite the fact that Clostridium butyricum (C. butyricum) is commonly used for treating ulcerative colitis (UC), the potential role of C. butyricum-secreted EVs in commensals-host crosstalk remains unclear.					
35578339	8	80	theme	gut	1742:1744	arg1	microbiota					1746:1755	gut microbiota	1742:1755	gut microbiota	1742:1755	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	2	81	theme	microbial	366:374	arg1	imbalance					376:384	microbial imbalance	366:384	microbial imbalance	366:384	And the molecules in EVs produced by procaryotes help in maintaining homeostasis, addressing microbial imbalance and infections, and regulating the immune system.					
35578339	7	82	theme	M2	1562:1563	arg1	macrophages					1565:1575	the M2 macrophages	1558:1575	the M2 macrophages	1558:1575	Furthermore, C. butyricum-derived EVs restored gut dysbiosis and altered the relative abundance of Helicobacter, Escherichia-Shigella, Lactobacillus, Akkermansia and Bacteroides, which, in turn, faecal transplantations from EVs-treated mice relieved the symptoms of UC and improved the impact of EVs on the reprogramming of the M2 macrophages.					
35578339	4	83	link	butyricum-derived	770:786	arg1	EVs					788:790	C. butyricum-derived EVs	767:790	C. butyricum-derived EVs	767:790	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
35578339	8	84	theme	macrophages	1695:1705	arg1	repolarization					1674:1687	the repolarization	1670:1687	the repolarization of M2 macrophages	1670:1705	CONCLUSION C. butyricum-derived EVs could protect against DSS-induced colitis by regulating the repolarization of M2 macrophages and remodelling the composition of gut microbiota, suggesting the potential efficacy of EVs from commensal and probiotic Clostridium species against UC.					
35578339	4	85	theme	microbiota	827:836	arg1	composition					838:848	gut microbiota composition	823:848	gut microbiota composition	823:848	RESULTS Here, we performed flow cytometry, western blot, immunohistochemistry and 16S rRNA analysis to explore the role of C. butyricum-derived EVs on macrophage polarization and gut microbiota composition in a dextran sulfate sodium (DSS)-induced UC mouse model.					
37003393	4	0	dep	suggested	485:493	arg1	occurred					517:524	occurred	517:524	suggested the main substitution occurred at the C-6 in the d-galactopyranose	485:560	13C NMR spectrum of the AB suggested the main substitution occurred at the C-6 in the d-galactopyranose.					
37003393	4	1	theme	13C	458:460	arg1	spectrum					466:473	13C NMR spectrum	458:473	13C NMR spectrum of the AB	458:483	13C NMR spectrum of the AB suggested the main substitution occurred at the C-6 in the d-galactopyranose.					
37003393	8	2	theme	obtained	980:987	arg1	AB					989:990	the obtained AB	976:990	the obtained AB	976:990	Moreover, the obtained AB possessed good emulsion stability.					
37003393	1	3	theme	aqueous	222:228	arg1	solution					230:237	aqueous solution	222:237	aqueous solution	222:237	Agar benzoate (AB) with different degrees of substitution (DS) was synthesized by the esterification of agar and benzoic anhydride in aqueous solution.					
37003393	0	4	theme	benzoate	78:85	arg1	characterization					11:26	characterization	11:26	characterization	11:26	Synthesis, characterization, antibacterial and emulsifying properties of agar benzoate.					
37003393	0	4	theme	benzoate	78:85	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, characterization, antibacterial and emulsifying properties of agar benzoate.					
37003393	0	4	theme	benzoate	78:85	arg1	antibacterial					29:41	antibacterial	29:41	antibacterial	29:41	Synthesis, characterization, antibacterial and emulsifying properties of agar benzoate.					
37003393	0	4	theme	benzoate	78:85	arg1	properties					59:68	emulsifying properties	47:68	emulsifying properties	47:68	Synthesis, characterization, antibacterial and emulsifying properties of agar benzoate.					
37003393	7	5	theme	AB	892:893	arg1	%					889:889	100 %	885:889	100 % (AB 20 g/L)	885:901	AB showed the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata, reaching 100 % (AB 20 g/L), 100 % (AB 40 g/L) and 19.35 % (7 d incubation), respectively.					
37003393	7	5	theme	AB	892:893	arg1	20 g/L					895:900	AB 20 g/L	892:900	AB 20 g/L	892:900	AB showed the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata, reaching 100 % (AB 20 g/L), 100 % (AB 40 g/L) and 19.35 % (7 d incubation), respectively.					
37003393	9	6	theme	application	1061:1071	arg1	prospects					1073:1081	broad application prospects	1055:1081	broad application prospects	1055:1081	These antibacterial AB have broad application prospects in the field of fruit and vegetable preservation.					
37003393	3	7	theme	chemical	323:330	arg1	structure					332:340	Its chemical structure	319:340	Its chemical structure	319:340	Its chemical structure was determined by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).					
37003393	5	8	theme	Cryo-scanning	563:575	arg1	Cryo-SEM					598:605	Cryo-SEM	598:605	Cryo-SEM	598:605	Cryo-scanning electron microscopy (Cryo-SEM) showed that the aperture of AB was larger than that of agar.					
37003393	5	8	theme	Cryo-scanning	563:575	arg1	microscopy					586:595	Cryo-scanning electron microscopy	563:595	Cryo-scanning electron microscopy (Cryo-SEM)	563:606	Cryo-scanning electron microscopy (Cryo-SEM) showed that the aperture of AB was larger than that of agar.					
37003393	7	9	theme	antibacterial	791:803	arg1	activity					805:812	the highest relative antibacterial activity	770:812	the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata	770:873	AB showed the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata, reaching 100 % (AB 20 g/L), 100 % (AB 40 g/L) and 19.35 % (7 d incubation), respectively.					
37003393	4	10	from	C-6	533:535	arg1	d-galactopyranose					544:560	the d-galactopyranose	540:560	the d-galactopyranose	540:560	13C NMR spectrum of the AB suggested the main substitution occurred at the C-6 in the d-galactopyranose.					
37003393	3	11	dep	Fourier	360:366	arg1	transform					368:376	transform	368:376	transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR)	368:455	Its chemical structure was determined by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).					
37003393	5	12	theme	electron	577:584	arg1	Cryo-SEM					598:605	Cryo-SEM	598:605	Cryo-SEM	598:605	Cryo-scanning electron microscopy (Cryo-SEM) showed that the aperture of AB was larger than that of agar.					
37003393	5	12	theme	electron	577:584	arg1	microscopy					586:595	Cryo-scanning electron microscopy	563:595	Cryo-scanning electron microscopy (Cryo-SEM)	563:606	Cryo-scanning electron microscopy (Cryo-SEM) showed that the aperture of AB was larger than that of agar.					
37003393	3	13	dep	transform	368:376	arg1	infrared					378:385	infrared	378:385	transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR)	368:455	Its chemical structure was determined by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).					
37003393	7	14	theme	highest	774:780	arg1	activity					805:812	the highest relative antibacterial activity	770:812	the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata	770:873	AB showed the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata, reaching 100 % (AB 20 g/L), 100 % (AB 40 g/L) and 19.35 % (7 d incubation), respectively.					
37003393	8	15	theme	emulsion	1007:1014	arg1	stability					1016:1024	good emulsion stability	1002:1024	good emulsion stability	1002:1024	Moreover, the obtained AB possessed good emulsion stability.					
37003393	8	16	theme	good	1002:1005	arg1	stability					1016:1024	good emulsion stability	1002:1024	good emulsion stability	1002:1024	Moreover, the obtained AB possessed good emulsion stability.					
37003393	1	17	theme	Agar	88:91	arg1	benzoate					93:100	Agar benzoate	88:100	Agar benzoate (AB) with different degrees of substitution (DS)	88:149	Agar benzoate (AB) with different degrees of substitution (DS) was synthesized by the esterification of agar and benzoic anhydride in aqueous solution.					
37003393	1	17	theme	Agar	88:91	arg1	AB					103:104	AB	103:104	AB	103:104	Agar benzoate (AB) with different degrees of substitution (DS) was synthesized by the esterification of agar and benzoic anhydride in aqueous solution.					
37003393	7	18	theme	relative	782:789	arg1	activity					805:812	the highest relative antibacterial activity	770:812	the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata	770:873	AB showed the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata, reaching 100 % (AB 20 g/L), 100 % (AB 40 g/L) and 19.35 % (7 d incubation), respectively.					
37003393	9	19	theme	broad	1055:1059	arg1	prospects					1073:1081	broad application prospects	1055:1081	broad application prospects	1055:1081	These antibacterial AB have broad application prospects in the field of fruit and vegetable preservation.					
37003393	2	20	theme	composition	279:289	arg1	ratio					291:295	composition ratio	279:295	composition ratio	279:295	The DS could be regulated by adjusting composition ratio, pH, and temperature.					
37003393	4	21	theme	main	499:502	arg1	substitution					504:515	the main substitution	495:515	the main substitution	495:515	13C NMR spectrum of the AB suggested the main substitution occurred at the C-6 in the d-galactopyranose.					
37003393	8	22	contain	possessed	992:1000	arg2	stability					1016:1024	good emulsion stability	1002:1024	good emulsion stability	1002:1024	Moreover, the obtained AB possessed good emulsion stability.					
37003393	8	22	contain	possessed	992:1000	arg1	AB					989:990	the obtained AB	976:990	the obtained AB	976:990	Moreover, the obtained AB possessed good emulsion stability.					
37003393	3	23	theme	resonance	428:436	arg1	NMR					452:454	NMR	452:454	NMR	452:454	Its chemical structure was determined by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).					
37003393	3	23	theme	resonance	428:436	arg1	spectroscopy					438:449	nuclear magnetic resonance spectroscopy	411:449	nuclear magnetic resonance spectroscopy (NMR)	411:455	Its chemical structure was determined by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).					
37003393	1	24	with	benzoate	93:100	arg1	degrees					122:128	different degrees	112:128	different degrees of substitution (DS)	112:149	Agar benzoate (AB) with different degrees of substitution (DS) was synthesized by the esterification of agar and benzoic anhydride in aqueous solution.					
37003393	9	25	theme	fruit	1099:1103	arg1	field					1090:1094	the field	1086:1094	the field of fruit and vegetable preservation	1086:1130	These antibacterial AB have broad application prospects in the field of fruit and vegetable preservation.					
37003393	3	26	theme	nuclear	411:417	arg1	resonance					428:436	nuclear magnetic resonance	411:436	nuclear magnetic resonance spectroscopy (NMR)	411:455	Its chemical structure was determined by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).					
37003393	4	27	theme	AB	482:483	arg1	spectrum					466:473	13C NMR spectrum	458:473	13C NMR spectrum of the AB	458:483	13C NMR spectrum of the AB suggested the main substitution occurred at the C-6 in the d-galactopyranose.					
37003393	7	28	theme	d	937:937	arg1	%					932:932	19.35 %	926:932	19.35 % (7 d incubation)	926:949	AB showed the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata, reaching 100 % (AB 20 g/L), 100 % (AB 40 g/L) and 19.35 % (7 d incubation), respectively.					
37003393	7	28	theme	d	937:937	arg1	incubation					939:948	7 d incubation	935:948	7 d incubation	935:948	AB showed the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata, reaching 100 % (AB 20 g/L), 100 % (AB 40 g/L) and 19.35 % (7 d incubation), respectively.					
37003393	9	29	theme	vegetable	1109:1117	arg1	field					1090:1094	the field	1086:1094	the field of fruit and vegetable preservation	1086:1130	These antibacterial AB have broad application prospects in the field of fruit and vegetable preservation.					
37003393	9	30	dep	fruit	1099:1103	arg1	preservation					1119:1130	preservation	1119:1130	preservation	1119:1130	These antibacterial AB have broad application prospects in the field of fruit and vegetable preservation.					
37003393	1	31	theme	agar	192:195	arg1	esterification					174:187	the esterification	170:187	the esterification of agar and benzoic anhydride in aqueous solution	170:237	Agar benzoate (AB) with different degrees of substitution (DS) was synthesized by the esterification of agar and benzoic anhydride in aqueous solution.					
37003393	0	32	theme	emulsifying	47:57	arg1	properties					59:68	emulsifying properties	47:68	emulsifying properties	47:68	Synthesis, characterization, antibacterial and emulsifying properties of agar benzoate.					
37003393	5	33	theme	AB	636:637	arg1	larger					643:648	larger	643:648	larger	643:648	Cryo-scanning electron microscopy (Cryo-SEM) showed that the aperture of AB was larger than that of agar.					
37003393	5	33	theme	AB	636:637	arg1	aperture					624:631	the aperture	620:631	the aperture of AB	620:637	Cryo-scanning electron microscopy (Cryo-SEM) showed that the aperture of AB was larger than that of agar.					
37003393	6	34	theme	AB	696:697	arg1	performance					681:691	The thermal performance	669:691	The thermal performance of AB	669:697	The thermal performance of AB decreased slightly, but this did not affect its performance.					
37003393	1	35	from	esterification	174:187	arg1	solution					230:237	aqueous solution	222:237	aqueous solution	222:237	Agar benzoate (AB) with different degrees of substitution (DS) was synthesized by the esterification of agar and benzoic anhydride in aqueous solution.					
37003393	1	36	theme	different	112:120	arg1	degrees					122:128	different degrees	112:128	different degrees of substitution (DS)	112:149	Agar benzoate (AB) with different degrees of substitution (DS) was synthesized by the esterification of agar and benzoic anhydride in aqueous solution.					
37003393	7	37	theme	Alternaria	854:863	arg1	alternata					865:873	Alternaria alternata	854:873	Alternaria alternata	854:873	AB showed the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata, reaching 100 % (AB 20 g/L), 100 % (AB 40 g/L) and 19.35 % (7 d incubation), respectively.					
37003393	7	38	theme	AB	911:912	arg1	%					908:908	100 %	904:908	100 % (AB 40 g/L)	904:920	AB showed the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata, reaching 100 % (AB 20 g/L), 100 % (AB 40 g/L) and 19.35 % (7 d incubation), respectively.					
37003393	7	38	theme	AB	911:912	arg1	40 g/L					914:919	AB 40 g/L	911:919	AB 40 g/L	911:919	AB showed the highest relative antibacterial activity against Escherichia coli, S. aureus and Alternaria alternata, reaching 100 % (AB 20 g/L), 100 % (AB 40 g/L) and 19.35 % (7 d incubation), respectively.					
37003393	1	39	theme	benzoic	201:207	arg1	anhydride					209:217	benzoic anhydride	201:217	benzoic anhydride	201:217	Agar benzoate (AB) with different degrees of substitution (DS) was synthesized by the esterification of agar and benzoic anhydride in aqueous solution.					
37003393	0	40	theme	agar	73:76	arg1	benzoate					78:85	agar benzoate	73:85	agar benzoate	73:85	Synthesis, characterization, antibacterial and emulsifying properties of agar benzoate.					
37003393	3	41	theme	magnetic	419:426	arg1	resonance					428:436	nuclear magnetic resonance	411:436	nuclear magnetic resonance spectroscopy (NMR)	411:455	Its chemical structure was determined by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).					
37003393	6	42	theme	thermal	673:679	arg1	performance					681:691	The thermal performance	669:691	The thermal performance of AB	669:697	The thermal performance of AB decreased slightly, but this did not affect its performance.					
37003393	1	43	theme	anhydride	209:217	arg1	esterification					174:187	the esterification	170:187	the esterification of agar and benzoic anhydride in aqueous solution	170:237	Agar benzoate (AB) with different degrees of substitution (DS) was synthesized by the esterification of agar and benzoic anhydride in aqueous solution.					
37003393	4	44	theme	NMR	462:464	arg1	spectrum					466:473	13C NMR spectrum	458:473	13C NMR spectrum of the AB	458:483	13C NMR spectrum of the AB suggested the main substitution occurred at the C-6 in the d-galactopyranose.					
37003393	9	45	theme	antibacterial	1033:1045	arg1	AB					1047:1048	These antibacterial AB	1027:1048	These antibacterial AB	1027:1048	These antibacterial AB have broad application prospects in the field of fruit and vegetable preservation.					
37003393	1	46	theme	substitution	133:144	arg1	degrees					122:128	different degrees	112:128	different degrees of substitution (DS)	112:149	Agar benzoate (AB) with different degrees of substitution (DS) was synthesized by the esterification of agar and benzoic anhydride in aqueous solution.					
37003393	9	47	contain	have	1050:1053	arg2	prospects					1073:1081	broad application prospects	1055:1081	broad application prospects	1055:1081	These antibacterial AB have broad application prospects in the field of fruit and vegetable preservation.					
37003393	9	47	contain	have	1050:1053	arg1	AB					1047:1048	These antibacterial AB	1027:1048	These antibacterial AB	1027:1048	These antibacterial AB have broad application prospects in the field of fruit and vegetable preservation.					
36724608	0	0	theme	culinaris	72:80	arg1	evaluation					38:47	Nutritional, chemical and antioxidant evaluation	0:47	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.	0:116	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	0	0	theme	culinaris	72:80	arg1	spp					82:84	Lens culinaris spp	67:84	Lens culinaris spp	67:84	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	9	1	theme	major	1187:1191	arg1	nutrients					1193:1201	the major nutrients	1183:1201	the major nutrients	1183:1201	Our results indicate that the growing season has a significant impact on the major nutrients in lentils such as the concentration of fat, ashes, fibers, and fructose and to a lesser extent proteins and sucrose.					
36724608	7	2	dep	63.7 	1035:1039	arg1	to					1032:1033	to	1032:1033	to	1032:1033	Fatty acid assessment showed the prevalence of PUFAs (45.3 to 63.7 %).					
36724608	3	3	theme	nutritional	266:276	arg1	profiles					291:298	The nutritional and chemical profiles	262:298	The nutritional and chemical profiles of 34 Armuña lentil samples	262:326	The nutritional and chemical profiles of 34 Armuña lentil samples were assessed together with their antioxidant capacity.					
36724608	9	4	theme	growing	1140:1146	arg1	season					1148:1153	the growing season	1136:1153	the growing season	1136:1153	Our results indicate that the growing season has a significant impact on the major nutrients in lentils such as the concentration of fat, ashes, fibers, and fructose and to a lesser extent proteins and sucrose.					
36724608	1	5	theme	Lens	127:130	arg1	Lentils					118:124	Lentils	118:124	Lentils (Lens culinaris spp.)	118:146	Lentils (Lens culinaris spp.)					
36724608	1	5	theme	Lens	127:130	arg1	spp					142:144	Lens culinaris spp.	127:145	Lens culinaris spp.	127:145	Lentils (Lens culinaris spp.)					
36724608	5	6	theme	valuable	622:629	arg1	nutrients					631:639	valuable nutrients	622:639	valuable nutrients	622:639	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	6	theme	valuable	622:629	arg1	carbohydrates					650:662	carbohydrates	650:662	carbohydrates	650:662	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	3	7	theme	34	303:304	arg1	samples					320:326	34 Armuña lentil samples	303:326	34 Armuña lentil samples	303:326	The nutritional and chemical profiles of 34 Armuña lentil samples were assessed together with their antioxidant capacity.					
36724608	4	8	theme	growing	454:460	arg1	season					462:467	the growing season	450:467	the growing season	450:467	In addition, the influence of both the climatic conditions during the growing season and the soil type in which they grow (Luvisol and Cambisol) on nutritional and chemical profiles was also evaluated.					
36724608	0	9	dep	evaluation	38:47	arg1	Influence					88:96	Influence	88:96	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.	0:116	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	5	10	dep	25.56 g/100 g	796:808	arg1	to					793:794	to	793:794	to	793:794	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	3	11	theme	Armuña	306:311	arg1	samples					320:326	34 Armuña lentil samples	303:326	34 Armuña lentil samples	303:326	The nutritional and chemical profiles of 34 Armuña lentil samples were assessed together with their antioxidant capacity.					
36724608	4	12	dep	grow	501:504	arg1	Cambisol					519:526	Cambisol	519:526	Cambisol	519:526	In addition, the influence of both the climatic conditions during the growing season and the soil type in which they grow (Luvisol and Cambisol) on nutritional and chemical profiles was also evaluated.					
36724608	4	12	dep	grow	501:504	arg1	Luvisol					507:513	Luvisol	507:513	Luvisol	507:513	In addition, the influence of both the climatic conditions during the growing season and the soil type in which they grow (Luvisol and Cambisol) on nutritional and chemical profiles was also evaluated.					
36724608	6	13	theme	oxalic	887:892	arg1	variety					874:880	this variety	869:880	this variety	869:880	Sucrose stood out as the main free sugar in this variety, and oxalic and γ-tocopherol as the main organic acid and tocopherol isoform respectively.					
36724608	9	14	theme	lesser	1285:1290	arg1	extent					1292:1297	a lesser extent proteins and sucrose	1283:1318	extent	1292:1297	Our results indicate that the growing season has a significant impact on the major nutrients in lentils such as the concentration of fat, ashes, fibers, and fructose and to a lesser extent proteins and sucrose.					
36724608	7	15	theme	acid	979:982	arg1	assessment					984:993	Fatty acid assessment	973:993	Fatty acid assessment	973:993	Fatty acid assessment showed the prevalence of PUFAs (45.3 to 63.7 %).					
36724608	6	16	theme	organic	923:929	arg1	acid					931:934	the main organic acid and tocopherol isoform	914:957	acid	931:934	Sucrose stood out as the main free sugar in this variety, and oxalic and γ-tocopherol as the main organic acid and tocopherol isoform respectively.					
36724608	9	17	theme	fibers	1255:1260	arg1	concentration					1226:1238	the concentration	1222:1238	the concentration of fat, ashes, fibers, and fructose	1222:1274	Our results indicate that the growing season has a significant impact on the major nutrients in lentils such as the concentration of fat, ashes, fibers, and fructose and to a lesser extent proteins and sucrose.					
36724608	8	18	theme	antioxidant	1051:1061	arg1	capacity					1063:1070	A high antioxidant capacity	1044:1070	A high antioxidant capacity (TBARS and OxHLIA)	1044:1089	A high antioxidant capacity (TBARS and OxHLIA) was also observed.					
36724608	1	19	theme	culinaris	132:140	arg1	Lentils					118:124	Lentils	118:124	Lentils (Lens culinaris spp.)	118:146	Lentils (Lens culinaris spp.)					
36724608	1	19	theme	culinaris	132:140	arg1	spp					142:144	Lens culinaris spp.	127:145	Lens culinaris spp.	127:145	Lentils (Lens culinaris spp.)					
36724608	7	20	theme	PUFAs	1020:1024	arg1	prevalence					1006:1015	the prevalence	1002:1015	the prevalence of PUFAs (45.3 to 63.7 %)	1002:1041	Fatty acid assessment showed the prevalence of PUFAs (45.3 to 63.7 %).					
36724608	4	21	theme	conditions	432:441	arg1	influence					401:409	the influence	397:409	the influence of both the climatic conditions during the growing season and the soil type in which they grow (Luvisol and Cambisol) on nutritional and chemical profiles	397:564	In addition, the influence of both the climatic conditions during the growing season and the soil type in which they grow (Luvisol and Cambisol) on nutritional and chemical profiles was also evaluated.					
36724608	9	22	from	impact	1173:1178	arg1	nutrients					1193:1201	the major nutrients	1183:1201	the major nutrients	1183:1201	Our results indicate that the growing season has a significant impact on the major nutrients in lentils such as the concentration of fat, ashes, fibers, and fructose and to a lesser extent proteins and sucrose.					
36724608	5	23	theme	proteins	777:784	arg1	fw					810:811	20.47 to 25.56 g/100 g fw	787:811	20.47 to 25.56 g/100 g fw	787:811	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	23	theme	proteins	777:784	arg1	ashes					818:822	ashes	818:822	ashes	818:822	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	23	theme	proteins	777:784	arg1	amounts					766:772	significant amounts	754:772	significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes	754:822	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	23	theme	proteins	777:784	arg1	amounts					611:617	large amounts	605:617	large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively	605:748	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	23	theme	proteins	777:784	arg1	carbohydrates					650:662	carbohydrates	650:662	carbohydrates	650:662	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	23	theme	proteins	777:784	arg1	proteins					777:784	proteins	777:784	proteins (20.47 to 25.56 g/100 g fw)	777:812	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	23	theme	proteins	777:784	arg1	nutrients					631:639	valuable nutrients	622:639	valuable nutrients	622:639	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	8	24	theme	high	1046:1049	arg1	capacity					1063:1070	A high antioxidant capacity	1044:1070	A high antioxidant capacity (TBARS and OxHLIA)	1044:1089	A high antioxidant capacity (TBARS and OxHLIA) was also observed.					
36724608	4	25	theme	climatic	423:430	arg1	conditions					432:441	the climatic conditions	419:441	the climatic conditions during the growing season	419:467	In addition, the influence of both the climatic conditions during the growing season and the soil type in which they grow (Luvisol and Cambisol) on nutritional and chemical profiles was also evaluated.					
36724608	9	26	theme	significant	1161:1171	arg1	impact					1173:1178	a significant impact	1159:1178	a significant impact on the major nutrients	1159:1201	Our results indicate that the growing season has a significant impact on the major nutrients in lentils such as the concentration of fat, ashes, fibers, and fructose and to a lesser extent proteins and sucrose.					
36724608	6	27	theme	tocopherol	940:949	arg1	isoform					951:957	the main organic acid and tocopherol isoform	914:957	isoform	951:957	Sucrose stood out as the main free sugar in this variety, and oxalic and γ-tocopherol as the main organic acid and tocopherol isoform respectively.					
36724608	0	28	theme	Nutritional	0:10	arg1	evaluation					38:47	Nutritional, chemical and antioxidant evaluation	0:47	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.	0:116	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	0	28	theme	Nutritional	0:10	arg1	spp					82:84	Lens culinaris spp	67:84	Lens culinaris spp	67:84	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	7	29	theme	Fatty	973:977	arg1	assessment					984:993	Fatty acid assessment	973:993	Fatty acid assessment	973:993	Fatty acid assessment showed the prevalence of PUFAs (45.3 to 63.7 %).					
36724608	9	30	theme	fat	1243:1245	arg1	concentration					1226:1238	the concentration	1222:1238	the concentration of fat, ashes, fibers, and fructose	1222:1274	Our results indicate that the growing season has a significant impact on the major nutrients in lentils such as the concentration of fat, ashes, fibers, and fructose and to a lesser extent proteins and sucrose.					
36724608	3	31	theme	lentil	313:318	arg1	samples					320:326	34 Armuña lentil samples	303:326	34 Armuña lentil samples	303:326	The nutritional and chemical profiles of 34 Armuña lentil samples were assessed together with their antioxidant capacity.					
36724608	0	32	theme	season	101:106	arg1	Influence					88:96	Influence	88:96	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.	0:116	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	2	33	theme	nutritional	242:252	arg1	assets					254:259	their valuable nutritional assets	227:259	their valuable nutritional assets	227:259	are a type of edible pulse consumed and produced worldwide; they are known for their valuable nutritional assets.					
36724608	3	34	theme	samples	320:326	arg1	profiles					291:298	The nutritional and chemical profiles	262:298	The nutritional and chemical profiles of 34 Armuña lentil samples	262:326	The nutritional and chemical profiles of 34 Armuña lentil samples were assessed together with their antioxidant capacity.					
36724608	6	35	theme	free	855:858	arg1	sugar					860:864	the main free sugar	846:864	the main free sugar in this variety, and oxalic and γ-tocopherol as the main organic acid and tocopherol isoform respectively	846:970	Sucrose stood out as the main free sugar in this variety, and oxalic and γ-tocopherol as the main organic acid and tocopherol isoform respectively.					
36724608	4	36	from	influence	401:409	arg1	profiles					557:564	nutritional and chemical profiles	532:564	nutritional and chemical profiles	532:564	In addition, the influence of both the climatic conditions during the growing season and the soil type in which they grow (Luvisol and Cambisol) on nutritional and chemical profiles was also evaluated.					
36724608	2	37	theme	valuable	233:240	arg1	assets					254:259	their valuable nutritional assets	227:259	their valuable nutritional assets	227:259	are a type of edible pulse consumed and produced worldwide; they are known for their valuable nutritional assets.					
36724608	4	38	theme	chemical	548:555	arg1	profiles					557:564	nutritional and chemical profiles	532:564	nutritional and chemical profiles	532:564	In addition, the influence of both the climatic conditions during the growing season and the soil type in which they grow (Luvisol and Cambisol) on nutritional and chemical profiles was also evaluated.					
36724608	6	39	theme	main	850:853	arg1	sugar					860:864	the main free sugar	846:864	the main free sugar in this variety, and oxalic and γ-tocopherol as the main organic acid and tocopherol isoform respectively	846:970	Sucrose stood out as the main free sugar in this variety, and oxalic and γ-tocopherol as the main organic acid and tocopherol isoform respectively.					
36724608	0	40	theme	soil	112:115	arg1	Influence					88:96	Influence	88:96	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.	0:116	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	6	41	theme	main	918:921	arg1	acid					931:934	the main organic acid and tocopherol isoform	914:957	acid	931:934	Sucrose stood out as the main free sugar in this variety, and oxalic and γ-tocopherol as the main organic acid and tocopherol isoform respectively.					
36724608	9	42	contain	has	1155:1157	arg2	impact					1173:1178	a significant impact	1159:1178	a significant impact on the major nutrients	1159:1201	Our results indicate that the growing season has a significant impact on the major nutrients in lentils such as the concentration of fat, ashes, fibers, and fructose and to a lesser extent proteins and sucrose.					
36724608	9	42	contain	has	1155:1157	arg1	season					1148:1153	the growing season	1136:1153	the growing season	1136:1153	Our results indicate that the growing season has a significant impact on the major nutrients in lentils such as the concentration of fat, ashes, fibers, and fructose and to a lesser extent proteins and sucrose.					
36724608	5	43	theme	significant	754:764	arg1	nutrients					631:639	valuable nutrients	622:639	valuable nutrients	622:639	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	43	theme	significant	754:764	arg1	ashes					818:822	ashes	818:822	ashes	818:822	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	43	theme	significant	754:764	arg1	amounts					766:772	significant amounts	754:772	significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes	754:822	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	43	theme	significant	754:764	arg1	fw					810:811	20.47 to 25.56 g/100 g fw	787:811	20.47 to 25.56 g/100 g fw	787:811	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	43	theme	significant	754:764	arg1	carbohydrates					650:662	carbohydrates	650:662	carbohydrates	650:662	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	43	theme	significant	754:764	arg1	proteins					777:784	proteins	777:784	proteins (20.47 to 25.56 g/100 g fw)	777:812	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	0	44	theme	antioxidant	26:36	arg1	evaluation					38:47	Nutritional, chemical and antioxidant evaluation	0:47	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.	0:116	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	0	44	theme	antioxidant	26:36	arg1	spp					82:84	Lens culinaris spp	67:84	Lens culinaris spp	67:84	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	3	45	theme	antioxidant	362:372	arg1	capacity					374:381	their antioxidant capacity	356:381	their antioxidant capacity	356:381	The nutritional and chemical profiles of 34 Armuña lentil samples were assessed together with their antioxidant capacity.					
36724608	10	46	from	types	1357:1361	arg1	study					1371:1375	this study	1366:1375	this study	1366:1375	In addition, the two different soil types in this study do not seem to affect any of the parameters analyzed.					
36724608	0	47	theme	Armuña	52:57	arg1	lentil					59:64	Armuña lentil	52:64	Armuña lentil	52:64	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	8	48	dep	capacity	1063:1070	arg1	TBARS					1073:1077	TBARS	1073:1077	TBARS	1073:1077	A high antioxidant capacity (TBARS and OxHLIA) was also observed.					
36724608	8	48	dep	capacity	1063:1070	arg1	OxHLIA					1083:1088	OxHLIA	1083:1088	OxHLIA	1083:1088	A high antioxidant capacity (TBARS and OxHLIA) was also observed.					
36724608	3	49	theme	chemical	282:289	arg1	profiles					291:298	The nutritional and chemical profiles	262:298	The nutritional and chemical profiles of 34 Armuña lentil samples	262:326	The nutritional and chemical profiles of 34 Armuña lentil samples were assessed together with their antioxidant capacity.					
36724608	2	50	theme	pulse	169:173	arg1	type					154:157	a type	152:157	a type of edible pulse consumed and produced worldwide	152:205	are a type of edible pulse consumed and produced worldwide; they are known for their valuable nutritional assets.					
36724608	4	51	theme	nutritional	532:542	arg1	profiles					557:564	nutritional and chemical profiles	532:564	nutritional and chemical profiles	532:564	In addition, the influence of both the climatic conditions during the growing season and the soil type in which they grow (Luvisol and Cambisol) on nutritional and chemical profiles was also evaluated.					
36724608	6	52	from	sugar	860:864	arg1	variety					874:880	this variety	869:880	this variety	869:880	Sucrose stood out as the main free sugar in this variety, and oxalic and γ-tocopherol as the main organic acid and tocopherol isoform respectively.					
36724608	4	53	theme	type	482:485	arg1	influence					401:409	the influence	397:409	the influence of both the climatic conditions during the growing season and the soil type in which they grow (Luvisol and Cambisol) on nutritional and chemical profiles	397:564	In addition, the influence of both the climatic conditions during the growing season and the soil type in which they grow (Luvisol and Cambisol) on nutritional and chemical profiles was also evaluated.					
36724608	5	54	theme	large	605:609	arg1	ashes					818:822	ashes	818:822	ashes	818:822	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	54	theme	large	605:609	arg1	fw					810:811	20.47 to 25.56 g/100 g fw	787:811	20.47 to 25.56 g/100 g fw	787:811	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	54	theme	large	605:609	arg1	nutrients					631:639	valuable nutrients	622:639	valuable nutrients	622:639	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	54	theme	large	605:609	arg1	amounts					611:617	large amounts	605:617	large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively	605:748	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	54	theme	large	605:609	arg1	carbohydrates					650:662	carbohydrates	650:662	carbohydrates	650:662	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	54	theme	large	605:609	arg1	proteins					777:784	proteins	777:784	proteins (20.47 to 25.56 g/100 g fw)	777:812	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	10	55	theme	different	1342:1350	arg1	types					1357:1361	the two different soil types	1334:1361	the two different soil types in this study	1334:1375	In addition, the two different soil types in this study do not seem to affect any of the parameters analyzed.					
36724608	2	56	theme	edible	162:167	arg1	pulse					169:173	edible pulse	162:173	edible pulse	162:173	are a type of edible pulse consumed and produced worldwide; they are known for their valuable nutritional assets.					
36724608	5	57	theme	ashes	818:822	arg1	fw					810:811	20.47 to 25.56 g/100 g fw	787:811	20.47 to 25.56 g/100 g fw	787:811	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	57	theme	ashes	818:822	arg1	ashes					818:822	ashes	818:822	ashes	818:822	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	57	theme	ashes	818:822	arg1	amounts					766:772	significant amounts	754:772	significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes	754:822	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	57	theme	ashes	818:822	arg1	amounts					611:617	large amounts	605:617	large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively	605:748	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	57	theme	ashes	818:822	arg1	carbohydrates					650:662	carbohydrates	650:662	carbohydrates	650:662	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	57	theme	ashes	818:822	arg1	proteins					777:784	proteins	777:784	proteins (20.47 to 25.56 g/100 g fw)	777:812	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	57	theme	ashes	818:822	arg1	nutrients					631:639	valuable nutrients	622:639	valuable nutrients	622:639	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	4	58	theme	soil	477:480	arg1	type					482:485	the soil type	473:485	the soil type	473:485	In addition, the influence of both the climatic conditions during the growing season and the soil type in which they grow (Luvisol and Cambisol) on nutritional and chemical profiles was also evaluated.					
36724608	10	59	theme	soil	1352:1355	arg1	types					1357:1361	the two different soil types	1334:1361	the two different soil types in this study	1334:1375	In addition, the two different soil types in this study do not seem to affect any of the parameters analyzed.					
36724608	9	60	theme	ashes	1248:1252	arg1	concentration					1226:1238	the concentration	1222:1238	the concentration of fat, ashes, fibers, and fructose	1222:1274	Our results indicate that the growing season has a significant impact on the major nutrients in lentils such as the concentration of fat, ashes, fibers, and fructose and to a lesser extent proteins and sucrose.					
36724608	9	61	dep	extent	1292:1297	arg1	proteins					1299:1306	proteins	1299:1306	proteins	1299:1306	Our results indicate that the growing season has a significant impact on the major nutrients in lentils such as the concentration of fat, ashes, fibers, and fructose and to a lesser extent proteins and sucrose.					
36724608	9	62	theme	fructose	1267:1274	arg1	concentration					1226:1238	the concentration	1222:1238	the concentration of fat, ashes, fibers, and fructose	1222:1274	Our results indicate that the growing season has a significant impact on the major nutrients in lentils such as the concentration of fat, ashes, fibers, and fructose and to a lesser extent proteins and sucrose.					
36724608	5	63	theme	nutrients	631:639	arg1	fw					810:811	20.47 to 25.56 g/100 g fw	787:811	20.47 to 25.56 g/100 g fw	787:811	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	63	theme	nutrients	631:639	arg1	ashes					818:822	ashes	818:822	ashes	818:822	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	63	theme	nutrients	631:639	arg1	amounts					766:772	significant amounts	754:772	significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes	754:822	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	63	theme	nutrients	631:639	arg1	amounts					611:617	large amounts	605:617	large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively	605:748	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	63	theme	nutrients	631:639	arg1	carbohydrates					650:662	carbohydrates	650:662	carbohydrates	650:662	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	63	theme	nutrients	631:639	arg1	proteins					777:784	proteins	777:784	proteins (20.47 to 25.56 g/100 g fw)	777:812	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	5	63	theme	nutrients	631:639	arg1	nutrients					631:639	valuable nutrients	622:639	valuable nutrients	622:639	Our results showed large amounts of valuable nutrients, such as carbohydrates, of which approximately 47.06 % and 29.11 % consist of fibers and starch respectively and significant amounts of proteins (20.47 to 25.56 g/100 g fw) and ashes.					
36724608	6	64	theme	γ-tocopherol	898:909	arg1	variety					874:880	this variety	869:880	this variety	869:880	Sucrose stood out as the main free sugar in this variety, and oxalic and γ-tocopherol as the main organic acid and tocopherol isoform respectively.					
36724608	0	65	theme	lentil	59:64	arg1	evaluation					38:47	Nutritional, chemical and antioxidant evaluation	0:47	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.	0:116	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	0	65	theme	lentil	59:64	arg1	spp					82:84	Lens culinaris spp	67:84	Lens culinaris spp	67:84	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	6	66	theme	Sucrose	825:831	arg1	stood					833:837	Sucrose stood	825:837	Sucrose stood	825:837	Sucrose stood out as the main free sugar in this variety, and oxalic and γ-tocopherol as the main organic acid and tocopherol isoform respectively.					
36724608	0	67	theme	chemical	13:20	arg1	evaluation					38:47	Nutritional, chemical and antioxidant evaluation	0:47	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.	0:116	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	0	67	theme	chemical	13:20	arg1	spp					82:84	Lens culinaris spp	67:84	Lens culinaris spp	67:84	Nutritional, chemical and antioxidant evaluation of Armuña lentil (Lens culinaris spp): Influence of season and soil.					
36724608	2	68	dep	type	154:157	arg1	consumed					175:182	consumed	175:182	consumed	175:182	are a type of edible pulse consumed and produced worldwide; they are known for their valuable nutritional assets.					
36724608	2	68	dep	type	154:157	arg1	produced					188:195	produced	188:195	produced worldwide	188:205	are a type of edible pulse consumed and produced worldwide; they are known for their valuable nutritional assets.					
36914014	2	0	theme	peripheral	307:316	arg1	responses					343:351	peripheral and central inflammatory responses	307:351	peripheral and central inflammatory responses to an immune challenge	307:374	There are marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice.					
36914014	7	1	theme	adult	1434:1438	arg1	mice					1440:1443	adult mice	1434:1443	adult mice that were pair-housed with an adult counterpart	1434:1491	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	4	2	theme	age-dependent	827:839	arg1	responses					848:856	age-dependent immune responses	827:856	age-dependent immune responses	827:856	The current study investigated whether cohousing adult and pubertal CD1 mice through three weeks of pair-housing, with the potential for microbiome exchange via coprophagy and other close contact, could mitigate age-dependent immune responses.					
36914014	7	3	theme	mRNA	1393:1396	arg1	expression					1398:1407	lower cytokine mRNA expression	1378:1407	lower cytokine mRNA expression in the brain	1378:1420	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	8	4	theme	cytokine	1589:1596	arg1	concentrations					1598:1611	peripheral cytokine concentrations	1578:1611	peripheral cytokine concentrations	1578:1611	However, when adult and pubertal mice were pair-housed, the age differences in both peripheral cytokine concentrations and central cytokine mRNA expression were mitigated.					
36914014	3	5	from	differences	573:583	arg1	composition					602:612	gut microbial composition	588:612	gut microbial composition	588:612	Given the strong link between the gut microbiome and immune system, it is possible that the age and sex differences in immune responses are mediated by age and sex differences in gut microbial composition.					
36914014	10	6	theme	potential	1924:1932	arg1	target					1946:1951	a potential therapeutic target	1922:1951	a potential therapeutic target	1922:1951	These results suggest that microbial composition could be involved in modulating these age-associated immune responses and thus may represent a potential therapeutic target.					
36914014	6	7	theme	LPS	1228:1230	arg1	treatment					1232:1240	LPS treatment	1228:1240	LPS treatment	1228:1240	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	1	8	theme	stress	236:241	arg1	systems					254:260	the stress and immune systems	232:260	systems	254:260	Puberty is a critical period of development that is marked by the maturation of the stress and immune systems.					
36914014	4	9	theme	other	791:795	arg1	contact					803:809	other close contact	791:809	other close contact	791:809	The current study investigated whether cohousing adult and pubertal CD1 mice through three weeks of pair-housing, with the potential for microbiome exchange via coprophagy and other close contact, could mitigate age-dependent immune responses.					
36914014	2	10	from	differences	292:302	arg1	responses					343:351	peripheral and central inflammatory responses	307:351	peripheral and central inflammatory responses to an immune challenge	307:374	There are marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice.					
36914014	7	11	theme	lower	1378:1382	arg1	expression					1398:1407	lower cytokine mRNA expression	1378:1407	lower cytokine mRNA expression in the brain	1378:1420	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	5	12	dep	challenge	985:993	arg1	LPS					1015:1017	LPS	1015:1017	LPS	1015:1017	Cytokine concentrations in the blood and cytokine mRNA expression in the brain were assessed following exposure to the immune challenge lipopolysaccharide (LPS).					
36914014	5	12	dep	challenge	985:993	arg1	lipopolysaccharide					995:1012	lipopolysaccharide	995:1012	the immune challenge lipopolysaccharide (LPS)	974:1018	Cytokine concentrations in the blood and cytokine mRNA expression in the brain were assessed following exposure to the immune challenge lipopolysaccharide (LPS).					
36914014	6	13	theme	mRNA	1126:1129	arg1	expression					1131:1140	central cytokine mRNA expression	1109:1140	central cytokine mRNA expression	1109:1140	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	9	14	theme	pair-housing	1685:1696	arg1	mice					1717:1720	pair-housing adult and pubertal mice	1685:1720	pair-housing adult and pubertal mice	1685:1720	We also found that pair-housing adult and pubertal mice eliminated the age difference in gut bacterial diversity.					
36914014	2	15	theme	marked	273:278	arg1	differences					292:302	marked age and sex differences	273:302	marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice	273:406	There are marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice.					
36914014	1	16	theme	critical	165:172	arg1	Puberty					152:158	Puberty	152:158	Puberty	152:158	Puberty is a critical period of development that is marked by the maturation of the stress and immune systems.					
36914014	1	16	theme	critical	165:172	arg1	period					174:179	a critical period	163:179	a critical period of development that is marked by the maturation of the stress and immune systems	163:260	Puberty is a critical period of development that is marked by the maturation of the stress and immune systems.					
36914014	6	17	theme	central	1109:1115	arg1	expression					1131:1140	central cytokine mRNA expression	1109:1140	central cytokine mRNA expression	1109:1140	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	7	18	theme	lower	1335:1339	arg1	concentrations					1350:1363	lower cytokine concentrations	1335:1363	lower cytokine concentrations in serum	1335:1372	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	4	19	theme	adult	664:668	arg1	mice					687:690	adult and pubertal CD1 mice	664:690	adult and pubertal CD1 mice	664:690	The current study investigated whether cohousing adult and pubertal CD1 mice through three weeks of pair-housing, with the potential for microbiome exchange via coprophagy and other close contact, could mitigate age-dependent immune responses.					
36914014	3	20	theme	immune	528:533	arg1	responses					535:543	immune responses	528:543	immune responses	528:543	Given the strong link between the gut microbiome and immune system, it is possible that the age and sex differences in immune responses are mediated by age and sex differences in gut microbial composition.					
36914014	7	21	from	concentrations	1350:1363	arg1	serum					1368:1372	serum	1368:1372	serum	1368:1372	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	7	21	from	concentrations	1350:1363	arg1	brain					1416:1420	the brain	1412:1420	the brain	1412:1420	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	9	22	theme	adult	1698:1702	arg1	mice					1717:1720	pair-housing adult and pubertal mice	1685:1720	pair-housing adult and pubertal mice	1685:1720	We also found that pair-housing adult and pubertal mice eliminated the age difference in gut bacterial diversity.					
36914014	7	23	theme	pubertal	1303:1310	arg1	counterpart					1312:1322	a pubertal counterpart	1301:1322	a pubertal counterpart	1301:1322	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	6	24	theme	prefrontal	1179:1188	arg1	PFC					1198:1200	PFC	1198:1200	PFC	1198:1200	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	6	24	theme	prefrontal	1179:1188	arg1	cortex					1190:1195	prefrontal cortex	1179:1195	prefrontal cortex (PFC)	1179:1201	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	4	25	theme	pubertal	674:681	arg1	mice					687:690	adult and pubertal CD1 mice	664:690	adult and pubertal CD1 mice	664:690	The current study investigated whether cohousing adult and pubertal CD1 mice through three weeks of pair-housing, with the potential for microbiome exchange via coprophagy and other close contact, could mitigate age-dependent immune responses.					
36914014	9	26	theme	pubertal	1708:1715	arg1	mice					1717:1720	pair-housing adult and pubertal mice	1685:1720	pair-housing adult and pubertal mice	1685:1720	We also found that pair-housing adult and pubertal mice eliminated the age difference in gut bacterial diversity.					
36914014	3	27	from	differences	513:523	arg1	responses					535:543	immune responses	528:543	immune responses	528:543	Given the strong link between the gut microbiome and immune system, it is possible that the age and sex differences in immune responses are mediated by age and sex differences in gut microbial composition.					
36914014	0	28	theme	female	52:57	arg1	mice					59:62	pair-housing pubertal and adult male and female mice	11:62	pair-housing pubertal and adult male and female mice	11:62	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	5	29	theme	Cytokine	859:866	arg1	concentrations					868:881	Cytokine concentrations	859:881	Cytokine concentrations in the blood	859:894	Cytokine concentrations in the blood and cytokine mRNA expression in the brain were assessed following exposure to the immune challenge lipopolysaccharide (LPS).					
36914014	9	30	theme	age	1737:1739	arg1	difference					1741:1750	the age difference	1733:1750	the age difference in gut bacterial diversity	1733:1777	We also found that pair-housing adult and pubertal mice eliminated the age difference in gut bacterial diversity.					
36914014	0	31	from	Effects	0:6	arg1	responses					100:108	LPS-induced age-dependent immune responses	67:108	LPS-induced age-dependent immune responses	67:108	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	0	32	theme	age-dependent	79:91	arg1	responses					100:108	LPS-induced age-dependent immune responses	67:108	LPS-induced age-dependent immune responses	67:108	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	8	33	from	differences	1558:1568	arg1	expression					1639:1648	central cytokine mRNA expression	1617:1648	central cytokine mRNA expression	1617:1648	However, when adult and pubertal mice were pair-housed, the age differences in both peripheral cytokine concentrations and central cytokine mRNA expression were mitigated.					
36914014	8	33	from	differences	1558:1568	arg1	concentrations					1598:1611	peripheral cytokine concentrations	1578:1611	peripheral cytokine concentrations	1578:1611	However, when adult and pubertal mice were pair-housed, the age differences in both peripheral cytokine concentrations and central cytokine mRNA expression were mitigated.					
36914014	2	34	theme	immune	359:364	arg1	challenge					366:374	an immune challenge	356:374	an immune challenge	356:374	There are marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice.					
36914014	9	35	theme	bacterial	1759:1767	arg1	diversity					1769:1777	gut bacterial diversity	1755:1777	gut bacterial diversity	1755:1777	We also found that pair-housing adult and pubertal mice eliminated the age difference in gut bacterial diversity.					
36914014	5	36	from	concentrations	868:881	arg1	brain					932:936	the brain	928:936	the brain	928:936	Cytokine concentrations in the blood and cytokine mRNA expression in the brain were assessed following exposure to the immune challenge lipopolysaccharide (LPS).					
36914014	5	36	from	concentrations	868:881	arg1	blood					890:894	the blood	886:894	the blood	886:894	Cytokine concentrations in the blood and cytokine mRNA expression in the brain were assessed following exposure to the immune challenge lipopolysaccharide (LPS).					
36914014	5	37	theme	cytokine	900:907	arg1	expression					914:923	cytokine mRNA expression	900:923	cytokine mRNA expression in the brain	900:936	Cytokine concentrations in the blood and cytokine mRNA expression in the brain were assessed following exposure to the immune challenge lipopolysaccharide (LPS).					
36914014	0	38	theme	pair-housing	11:22	arg1	mice					59:62	pair-housing pubertal and adult male and female mice	11:62	pair-housing pubertal and adult male and female mice	11:62	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	1	39	theme	systems	254:260	arg1	maturation					218:227	the maturation	214:227	the maturation of the stress and immune systems	214:260	Puberty is a critical period of development that is marked by the maturation of the stress and immune systems.					
36914014	8	40	theme	adult	1508:1512	arg1	mice					1527:1530	adult and pubertal mice	1508:1530	adult and pubertal mice	1508:1530	However, when adult and pubertal mice were pair-housed, the age differences in both peripheral cytokine concentrations and central cytokine mRNA expression were mitigated.					
36914014	3	41	theme	gut	588:590	arg1	composition					602:612	gut microbial composition	588:612	gut microbial composition	588:612	Given the strong link between the gut microbiome and immune system, it is possible that the age and sex differences in immune responses are mediated by age and sex differences in gut microbial composition.					
36914014	4	42	dep	mitigate	818:825	arg1	cohousing					654:662	cohousing	654:662	cohousing adult and pubertal CD1 mice through three weeks of pair-housing	654:726	The current study investigated whether cohousing adult and pubertal CD1 mice through three weeks of pair-housing, with the potential for microbiome exchange via coprophagy and other close contact, could mitigate age-dependent immune responses.					
36914014	7	43	theme	adult	1475:1479	arg1	counterpart					1481:1491	an adult counterpart	1472:1491	an adult counterpart	1472:1491	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	5	44	theme	immune	978:983	arg1	challenge					985:993	the immune challenge lipopolysaccharide (LPS)	974:1018	the immune challenge lipopolysaccharide (LPS)	974:1018	Cytokine concentrations in the blood and cytokine mRNA expression in the brain were assessed following exposure to the immune challenge lipopolysaccharide (LPS).					
36914014	8	45	theme	pubertal	1518:1525	arg1	mice					1527:1530	adult and pubertal mice	1508:1530	adult and pubertal mice	1508:1530	However, when adult and pubertal mice were pair-housed, the age differences in both peripheral cytokine concentrations and central cytokine mRNA expression were mitigated.					
36914014	0	46	theme	male	43:46	arg1	mice					59:62	pair-housing pubertal and adult male and female mice	11:62	pair-housing pubertal and adult male and female mice	11:62	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	10	47	theme	microbial	1807:1815	arg1	composition					1817:1827	microbial composition	1807:1827	microbial composition	1807:1827	These results suggest that microbial composition could be involved in modulating these age-associated immune responses and thus may represent a potential therapeutic target.					
36914014	7	48	theme	Pubertal	1243:1250	arg1	mice					1268:1271	Pubertal male and female mice	1243:1271	Pubertal male and female mice	1243:1271	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	2	49	theme	sex	288:290	arg1	differences					292:302	marked age and sex differences	273:302	marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice	273:406	There are marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice.					
36914014	5	50	from	expression	914:923	arg1	brain					932:936	the brain	928:936	the brain	928:936	Cytokine concentrations in the blood and cytokine mRNA expression in the brain were assessed following exposure to the immune challenge lipopolysaccharide (LPS).					
36914014	5	50	from	expression	914:923	arg1	blood					890:894	the blood	886:894	the blood	886:894	Cytokine concentrations in the blood and cytokine mRNA expression in the brain were assessed following exposure to the immune challenge lipopolysaccharide (LPS).					
36914014	0	51	dep	pair-housing	11:22	arg1	pubertal					24:31	pubertal	24:31	pubertal	24:31	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	6	52	theme	cytokine	1072:1079	arg1	concentrations					1081:1094	increased cytokine concentrations	1062:1094	increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC)	1062:1201	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	2	53	theme	inflammatory	330:341	arg1	responses					343:351	peripheral and central inflammatory responses	307:351	peripheral and central inflammatory responses to an immune challenge	307:374	There are marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice.					
36914014	8	54	theme	central	1617:1623	arg1	expression					1639:1648	central cytokine mRNA expression	1617:1648	central cytokine mRNA expression	1617:1648	However, when adult and pubertal mice were pair-housed, the age differences in both peripheral cytokine concentrations and central cytokine mRNA expression were mitigated.					
36914014	0	55	dep	Effects	0:6	arg1	role					123:126	A potential role	111:126	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.	0:150	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	8	56	theme	age	1554:1556	arg1	differences					1558:1568	the age differences	1550:1568	the age differences in both peripheral cytokine concentrations and central cytokine mRNA expression	1550:1648	However, when adult and pubertal mice were pair-housed, the age differences in both peripheral cytokine concentrations and central cytokine mRNA expression were mitigated.					
36914014	3	57	theme	immune	462:467	arg1	system					469:474	the gut microbiome and immune system	439:474	system	469:474	Given the strong link between the gut microbiome and immune system, it is possible that the age and sex differences in immune responses are mediated by age and sex differences in gut microbial composition.					
36914014	8	58	theme	mRNA	1634:1637	arg1	expression					1639:1648	central cytokine mRNA expression	1617:1648	central cytokine mRNA expression	1617:1648	However, when adult and pubertal mice were pair-housed, the age differences in both peripheral cytokine concentrations and central cytokine mRNA expression were mitigated.					
36914014	10	59	theme	immune	1882:1887	arg1	responses					1889:1897	these age-associated immune responses	1861:1897	these age-associated immune responses	1861:1897	These results suggest that microbial composition could be involved in modulating these age-associated immune responses and thus may represent a potential therapeutic target.					
36914014	3	60	theme	sex	509:511	arg1	differences					513:523	the age and sex differences	497:523	differences	513:523	Given the strong link between the gut microbiome and immune system, it is possible that the age and sex differences in immune responses are mediated by age and sex differences in gut microbial composition.					
36914014	8	61	theme	peripheral	1578:1587	arg1	concentrations					1598:1611	peripheral cytokine concentrations	1578:1611	peripheral cytokine concentrations	1578:1611	However, when adult and pubertal mice were pair-housed, the age differences in both peripheral cytokine concentrations and central cytokine mRNA expression were mitigated.					
36914014	7	62	theme	cytokine	1384:1391	arg1	expression					1398:1407	lower cytokine mRNA expression	1378:1407	lower cytokine mRNA expression in the brain	1378:1420	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	2	63	theme	age	280:282	arg1	differences					292:302	marked age and sex differences	273:302	marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice	273:406	There are marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice.					
36914014	4	64	theme	close	797:801	arg1	contact					803:809	other close contact	791:809	other close contact	791:809	The current study investigated whether cohousing adult and pubertal CD1 mice through three weeks of pair-housing, with the potential for microbiome exchange via coprophagy and other close contact, could mitigate age-dependent immune responses.					
36914014	6	65	theme	cytokine	1117:1124	arg1	expression					1131:1140	central cytokine mRNA expression	1109:1140	central cytokine mRNA expression	1109:1140	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	0	66	theme	potential	113:121	arg1	role					123:126	A potential role	111:126	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.	0:150	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	3	67	theme	age	501:503	arg1	differences					513:523	the age and sex differences	497:523	differences	513:523	Given the strong link between the gut microbiome and immune system, it is possible that the age and sex differences in immune responses are mediated by age and sex differences in gut microbial composition.					
36914014	10	68	theme	therapeutic	1934:1944	arg1	target					1946:1951	a potential therapeutic target	1922:1951	a potential therapeutic target	1922:1951	These results suggest that microbial composition could be involved in modulating these age-associated immune responses and thus may represent a potential therapeutic target.					
36914014	1	69	theme	development	184:194	arg1	Puberty					152:158	Puberty	152:158	Puberty	152:158	Puberty is a critical period of development that is marked by the maturation of the stress and immune systems.					
36914014	1	69	theme	development	184:194	arg1	period					174:179	a critical period	163:179	a critical period of development that is marked by the maturation of the stress and immune systems	163:260	Puberty is a critical period of development that is marked by the maturation of the stress and immune systems.					
36914014	7	70	theme	cytokine	1341:1348	arg1	concentrations					1350:1363	lower cytokine concentrations	1335:1363	lower cytokine concentrations in serum	1335:1372	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	0	71	theme	gut	136:138	arg1	microbiota					140:149	the gut microbiota	132:149	the gut microbiota	132:149	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	4	72	theme	immune	841:846	arg1	responses					848:856	age-dependent immune responses	827:856	age-dependent immune responses	827:856	The current study investigated whether cohousing adult and pubertal CD1 mice through three weeks of pair-housing, with the potential for microbiome exchange via coprophagy and other close contact, could mitigate age-dependent immune responses.					
36914014	3	73	theme	age	561:563	arg1	differences					573:583	age and sex differences	561:583	age and sex differences in gut microbial composition	561:612	Given the strong link between the gut microbiome and immune system, it is possible that the age and sex differences in immune responses are mediated by age and sex differences in gut microbial composition.					
36914014	0	74	theme	mice	59:62	arg1	Effects					0:6	Effects	0:6	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.	0:150	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	4	75	theme	current	619:625	arg1	study					627:631	The current study	615:631	The current study	615:631	The current study investigated whether cohousing adult and pubertal CD1 mice through three weeks of pair-housing, with the potential for microbiome exchange via coprophagy and other close contact, could mitigate age-dependent immune responses.					
36914014	0	76	theme	LPS-induced	67:77	arg1	responses					100:108	LPS-induced age-dependent immune responses	67:108	LPS-induced age-dependent immune responses	67:108	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	0	77	theme	immune	93:98	arg1	responses					100:108	LPS-induced age-dependent immune responses	67:108	LPS-induced age-dependent immune responses	67:108	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	7	78	from	expression	1398:1407	arg1	serum					1368:1372	serum	1368:1372	serum	1368:1372	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	7	78	from	expression	1398:1407	arg1	brain					1416:1420	the brain	1412:1420	the brain	1412:1420	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	9	79	theme	gut	1755:1757	arg1	diversity					1769:1777	gut bacterial diversity	1755:1777	gut bacterial diversity	1755:1777	We also found that pair-housing adult and pubertal mice eliminated the age difference in gut bacterial diversity.					
36914014	4	80	theme	pair-housing	715:726	arg1	weeks					706:710	three weeks	700:710	three weeks of pair-housing	700:726	The current study investigated whether cohousing adult and pubertal CD1 mice through three weeks of pair-housing, with the potential for microbiome exchange via coprophagy and other close contact, could mitigate age-dependent immune responses.					
36914014	4	81	theme	CD1	683:685	arg1	mice					687:690	adult and pubertal CD1 mice	664:690	adult and pubertal CD1 mice	664:690	The current study investigated whether cohousing adult and pubertal CD1 mice through three weeks of pair-housing, with the potential for microbiome exchange via coprophagy and other close contact, could mitigate age-dependent immune responses.					
36914014	1	82	theme	immune	247:252	arg1	systems					254:260	the stress and immune systems	232:260	systems	254:260	Puberty is a critical period of development that is marked by the maturation of the stress and immune systems.					
36914014	5	83	theme	mRNA	909:912	arg1	expression					914:923	cytokine mRNA expression	900:923	cytokine mRNA expression in the brain	900:936	Cytokine concentrations in the blood and cytokine mRNA expression in the brain were assessed following exposure to the immune challenge lipopolysaccharide (LPS).					
36914014	7	84	theme	female	1261:1266	arg1	mice					1268:1271	Pubertal male and female mice	1243:1271	Pubertal male and female mice	1243:1271	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	2	85	theme	adult	397:401	arg1	mice					403:406	pubertal and adult mice	384:406	pubertal and adult mice	384:406	There are marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice.					
36914014	2	86	theme	pubertal	384:391	arg1	mice					403:406	pubertal and adult mice	384:406	pubertal and adult mice	384:406	There are marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice.					
36914014	0	87	theme	adult	37:41	arg1	mice					59:62	pair-housing pubertal and adult male and female mice	11:62	pair-housing pubertal and adult male and female mice	11:62	Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota.					
36914014	7	88	theme	male	1252:1255	arg1	mice					1268:1271	Pubertal male and female mice	1243:1271	Pubertal male and female mice	1243:1271	Pubertal male and female mice, that were pair-housed with a pubertal counterpart, displayed lower cytokine concentrations in serum and lower cytokine mRNA expression in the brain compared to adult mice that were pair-housed with an adult counterpart.					
36914014	3	89	theme	sex	569:571	arg1	differences					573:583	age and sex differences	561:583	age and sex differences in gut microbial composition	561:612	Given the strong link between the gut microbiome and immune system, it is possible that the age and sex differences in immune responses are mediated by age and sex differences in gut microbial composition.					
36914014	4	90	theme	microbiome	752:761	arg1	exchange					763:770	microbiome exchange	752:770	microbiome exchange	752:770	The current study investigated whether cohousing adult and pubertal CD1 mice through three weeks of pair-housing, with the potential for microbiome exchange via coprophagy and other close contact, could mitigate age-dependent immune responses.					
36914014	6	91	from	concentrations	1081:1094	arg1	expression					1131:1140	central cytokine mRNA expression	1109:1140	central cytokine mRNA expression	1109:1140	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	6	91	from	concentrations	1081:1094	arg1	serum					1099:1103	serum	1099:1103	serum	1099:1103	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	6	91	from	concentrations	1081:1094	arg1	cortex					1190:1195	prefrontal cortex	1179:1195	prefrontal cortex (PFC)	1179:1201	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	6	91	from	concentrations	1081:1094	arg1	hippocampus					1149:1159	the hippocampus	1145:1159	the hippocampus	1145:1159	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	6	91	from	concentrations	1081:1094	arg1	PFC					1198:1200	PFC	1198:1200	PFC	1198:1200	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	6	91	from	concentrations	1081:1094	arg1	hypothalamus					1162:1173	hypothalamus	1162:1173	hypothalamus	1162:1173	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	3	92	theme	strong	419:424	arg1	link					426:429	the strong link	415:429	the strong link between the gut microbiome and immune system	415:474	Given the strong link between the gut microbiome and immune system, it is possible that the age and sex differences in immune responses are mediated by age and sex differences in gut microbial composition.					
36914014	9	93	from	difference	1741:1750	arg1	diversity					1769:1777	gut bacterial diversity	1755:1777	gut bacterial diversity	1755:1777	We also found that pair-housing adult and pubertal mice eliminated the age difference in gut bacterial diversity.					
36914014	10	94	theme	age-associated	1867:1880	arg1	responses					1889:1897	these age-associated immune responses	1861:1897	these age-associated immune responses	1861:1897	These results suggest that microbial composition could be involved in modulating these age-associated immune responses and thus may represent a potential therapeutic target.					
36914014	3	95	theme	microbial	592:600	arg1	composition					602:612	gut microbial composition	588:612	gut microbial composition	588:612	Given the strong link between the gut microbiome and immune system, it is possible that the age and sex differences in immune responses are mediated by age and sex differences in gut microbial composition.					
36914014	3	96	theme	gut	443:445	arg1	microbiome					447:456	the gut microbiome and immune system	439:474	microbiome	447:456	Given the strong link between the gut microbiome and immune system, it is possible that the age and sex differences in immune responses are mediated by age and sex differences in gut microbial composition.					
36914014	8	97	theme	cytokine	1625:1632	arg1	expression					1639:1648	central cytokine mRNA expression	1617:1648	central cytokine mRNA expression	1617:1648	However, when adult and pubertal mice were pair-housed, the age differences in both peripheral cytokine concentrations and central cytokine mRNA expression were mitigated.					
36914014	6	98	theme	increased	1062:1070	arg1	concentrations					1081:1094	increased cytokine concentrations	1062:1094	increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC)	1062:1201	The results show that all mice displayed increased cytokine concentrations in serum and central cytokine mRNA expression in the hippocampus, hypothalamus and prefrontal cortex (PFC) at eight hours following LPS treatment.					
36914014	2	99	theme	central	322:328	arg1	responses					343:351	peripheral and central inflammatory responses	307:351	peripheral and central inflammatory responses to an immune challenge	307:374	There are marked age and sex differences in peripheral and central inflammatory responses to an immune challenge between pubertal and adult mice.					
36126824	10	0	used	used	1294:1297	arg2	systems					1271:1277	These organogel systems	1255:1277	These organogel systems	1255:1277	These organogel systems can further be used for developing 3D printed drug delivery systems or scaffolds for tissue engineering.					
36126824	5	1	theme	zein	613:616	arg1	self-assembly					596:608	water-induced self-assembly	582:608	water-induced self-assembly of zein	582:616	Furthermore, water-induced self-assembly of zein allows mechanically robust in situ implant formation by solvent exchange.					
36126824	7	2	dep	In	816:817	arg1	situ					819:822	situ	819:822	situ	819:822	In situ implants in agarose gel retained antibacterial efficacy against S. aureus for more than 14 days.					
36126824	3	3	theme	bonding	424:430	arg1	component					432:440	the hydrogen bonding component	411:440	the hydrogen bonding component of Hansen Solubility Parameters	411:472	The gel formation was driven by the solvent's polarity and the hydrogen bonding component of Hansen Solubility Parameters was important in promoting gelation.					
36126824	3	3	theme	bonding	424:430	arg1	important					478:486	important	478:486	important	478:486	The gel formation was driven by the solvent's polarity and the hydrogen bonding component of Hansen Solubility Parameters was important in promoting gelation.					
36126824	5	4	theme	solvent	674:680	arg1	exchange					682:689	solvent exchange	674:689	solvent exchange	674:689	Furthermore, water-induced self-assembly of zein allows mechanically robust in situ implant formation by solvent exchange.					
36126824	10	5	theme	tissue	1364:1369	arg1	engineering					1371:1381	tissue engineering	1364:1381	tissue engineering	1364:1381	These organogel systems can further be used for developing 3D printed drug delivery systems or scaffolds for tissue engineering.					
36126824	8	6	theme	zein	1005:1008	arg1	gel					1010:1012	zein gel	1005:1012	zein gel in DMSO	1005:1020	Zein-based organogels were further applied as 3D printing ink and it was found that zein gel in DMSO had superior printability than gels prepared in NMP and GF.					
36126824	0	7	theme	printing	86:93	arg1	ink					95:97	3D printing ink	83:97	3D printing ink	83:97	Self-assembled zein organogels as in situ forming implant drug delivery system and 3D printing ink.					
36126824	6	8	theme	exchange	788:795	arg1	rate					797:800	the solvent exchange rate	776:800	the solvent exchange rate	776:800	Ciprofloxacin was incorporated as a model drug and sustained release depending upon the solvent exchange rate was observed.					
36126824	10	9	theme	printed	1317:1323	arg1	systems					1339:1345	3D printed drug delivery systems	1314:1345	3D printed drug delivery systems	1314:1345	These organogel systems can further be used for developing 3D printed drug delivery systems or scaffolds for tissue engineering.					
36126824	0	10	theme	3D	83:84	arg1	ink					95:97	3D printing ink	83:97	3D printing ink	83:97	Self-assembled zein organogels as in situ forming implant drug delivery system and 3D printing ink.					
36126824	5	11	theme	robust	638:643	arg1	formation					661:669	mechanically robust in situ implant formation	625:669	mechanically robust in situ implant formation	625:669	Furthermore, water-induced self-assembly of zein allows mechanically robust in situ implant formation by solvent exchange.					
36126824	6	12	theme	solvent	780:786	arg1	rate					797:800	the solvent exchange rate	776:800	the solvent exchange rate	776:800	Ciprofloxacin was incorporated as a model drug and sustained release depending upon the solvent exchange rate was observed.					
36126824	10	13	theme	organogel	1261:1269	arg1	systems					1271:1277	These organogel systems	1255:1277	These organogel systems	1255:1277	These organogel systems can further be used for developing 3D printed drug delivery systems or scaffolds for tissue engineering.					
36126824	4	14	theme	shear-thinning	526:539	arg1	properties					557:566	shear-thinning and thixotropic properties	526:566	shear-thinning and thixotropic properties	526:566	Gels exhibited shear-thinning and thixotropic properties.					
36126824	5	15	dep	in	645:646	arg1	situ					648:651	situ	648:651	situ	648:651	Furthermore, water-induced self-assembly of zein allows mechanically robust in situ implant formation by solvent exchange.					
36126824	8	16	theme	3D	967:968	arg1	organogels					932:941	Zein-based organogels	921:941	Zein-based organogels	921:941	Zein-based organogels were further applied as 3D printing ink and it was found that zein gel in DMSO had superior printability than gels prepared in NMP and GF.					
36126824	8	16	theme	3D	967:968	arg1	ink					979:981	3D printing ink	967:981	3D printing ink	967:981	Zein-based organogels were further applied as 3D printing ink and it was found that zein gel in DMSO had superior printability than gels prepared in NMP and GF.					
36126824	2	17	theme	zein	252:255	arg1	organogels					257:266	self-assembled zein organogels	237:266	self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF)	237:349	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	7	18	theme	agarose	836:842	arg1	gel					844:846	agarose gel	836:846	agarose gel	836:846	In situ implants in agarose gel retained antibacterial efficacy against S. aureus for more than 14 days.					
36126824	8	19	contain	had	1022:1024	arg2	printability					1035:1046	superior printability	1026:1046	superior printability	1026:1046	Zein-based organogels were further applied as 3D printing ink and it was found that zein gel in DMSO had superior printability than gels prepared in NMP and GF.					
36126824	8	19	contain	had	1022:1024	arg1	gel					1010:1012	zein gel	1005:1012	zein gel in DMSO	1005:1020	Zein-based organogels were further applied as 3D printing ink and it was found that zein gel in DMSO had superior printability than gels prepared in NMP and GF.					
36126824	5	20	theme	water-induced	582:594	arg1	self-assembly					596:608	water-induced self-assembly	582:608	water-induced self-assembly of zein	582:616	Furthermore, water-induced self-assembly of zein allows mechanically robust in situ implant formation by solvent exchange.					
36126824	9	21	theme	various	1157:1163	arg1	drugs					1165:1169	various drugs	1157:1169	various drugs	1157:1169	Using three solvents to prepare organogels can enable the encapsulation of various drugs and facilitate the preparation of composite gels with other biocompatible polymers.					
36126824	2	22	theme	self-assembled	237:250	arg1	organogels					257:266	self-assembled zein organogels	237:266	self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF)	237:349	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	9	23	with	gels	1215:1218	arg1	polymers					1245:1252	other biocompatible polymers	1225:1252	other biocompatible polymers	1225:1252	Using three solvents to prepare organogels can enable the encapsulation of various drugs and facilitate the preparation of composite gels with other biocompatible polymers.					
36126824	2	24	theme	formal	339:344	arg1	glycerol					330:337	glycerol formal	330:344	glycerol formal (GF)	330:349	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	2	24	theme	formal	339:344	arg1	GF					347:348	GF	347:348	GF	347:348	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	8	25	theme	superior	1026:1033	arg1	printability					1035:1046	superior printability	1026:1046	superior printability	1026:1046	Zein-based organogels were further applied as 3D printing ink and it was found that zein gel in DMSO had superior printability than gels prepared in NMP and GF.					
36126824	9	26	theme	drugs	1165:1169	arg1	encapsulation					1140:1152	the encapsulation	1136:1152	the encapsulation of various drugs	1136:1169	Using three solvents to prepare organogels can enable the encapsulation of various drugs and facilitate the preparation of composite gels with other biocompatible polymers.					
36126824	3	27	theme	gel	356:358	arg1	formation					360:368	The gel formation	352:368	The gel formation	352:368	The gel formation was driven by the solvent's polarity and the hydrogen bonding component of Hansen Solubility Parameters was important in promoting gelation.					
36126824	0	28	theme	Self-assembled	0:13	arg1	zein					15:18	Self-assembled zein	0:18	Self-assembled zein	0:18	Self-assembled zein organogels as in situ forming implant drug delivery system and 3D printing ink.					
36126824	8	29	from	gel	1010:1012	arg1	DMSO					1017:1020	DMSO	1017:1020	DMSO	1017:1020	Zein-based organogels were further applied as 3D printing ink and it was found that zein gel in DMSO had superior printability than gels prepared in NMP and GF.					
36126824	4	30	theme	thixotropic	545:555	arg1	properties					557:566	shear-thinning and thixotropic properties	526:566	shear-thinning and thixotropic properties	526:566	Gels exhibited shear-thinning and thixotropic properties.					
36126824	6	31	theme	sustained	743:751	arg1	release					753:759	sustained release	743:759	sustained release depending upon the solvent exchange rate	743:800	Ciprofloxacin was incorporated as a model drug and sustained release depending upon the solvent exchange rate was observed.					
36126824	7	32	theme	In	816:817	arg1	implants					824:831	In situ implants	816:831	In situ implants in agarose gel	816:846	In situ implants in agarose gel retained antibacterial efficacy against S. aureus for more than 14 days.					
36126824	10	33	theme	delivery	1330:1337	arg1	systems					1339:1345	3D printed drug delivery systems	1314:1345	3D printed drug delivery systems	1314:1345	These organogel systems can further be used for developing 3D printed drug delivery systems or scaffolds for tissue engineering.					
36126824	3	34	theme	Solubility	452:461	arg1	Parameters					463:472	Hansen Solubility Parameters	445:472	Hansen Solubility Parameters	445:472	The gel formation was driven by the solvent's polarity and the hydrogen bonding component of Hansen Solubility Parameters was important in promoting gelation.					
36126824	2	35	theme	N-methyl	271:278	arg1	pyrrolidone					280:290	N-methyl pyrrolidone	271:290	N-methyl pyrrolidone (NMP)	271:296	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	2	35	theme	N-methyl	271:278	arg1	NMP					293:295	NMP	293:295	NMP	293:295	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	3	36	theme	hydrogen	415:422	arg1	bonding					424:430	hydrogen bonding	415:430	the hydrogen bonding component of Hansen Solubility Parameters	411:472	The gel formation was driven by the solvent's polarity and the hydrogen bonding component of Hansen Solubility Parameters was important in promoting gelation.					
36126824	0	37	theme	in	34:35	arg1	system					72:77	in situ forming implant drug delivery system	34:77	in situ forming implant drug delivery system	34:77	Self-assembled zein organogels as in situ forming implant drug delivery system and 3D printing ink.					
36126824	8	38	theme	printing	970:977	arg1	organogels					932:941	Zein-based organogels	921:941	Zein-based organogels	921:941	Zein-based organogels were further applied as 3D printing ink and it was found that zein gel in DMSO had superior printability than gels prepared in NMP and GF.					
36126824	8	38	theme	printing	970:977	arg1	ink					979:981	3D printing ink	967:981	3D printing ink	967:981	Zein-based organogels were further applied as 3D printing ink and it was found that zein gel in DMSO had superior printability than gels prepared in NMP and GF.					
36126824	10	39	theme	3D	1314:1315	arg1	systems					1339:1345	3D printed drug delivery systems	1314:1345	3D printed drug delivery systems	1314:1345	These organogel systems can further be used for developing 3D printed drug delivery systems or scaffolds for tissue engineering.					
36126824	0	40	theme	implant	50:56	arg1	system					72:77	in situ forming implant drug delivery system	34:77	in situ forming implant drug delivery system	34:77	Self-assembled zein organogels as in situ forming implant drug delivery system and 3D printing ink.					
36126824	5	41	theme	in	645:646	arg1	formation					661:669	mechanically robust in situ implant formation	625:669	mechanically robust in situ implant formation	625:669	Furthermore, water-induced self-assembly of zein allows mechanically robust in situ implant formation by solvent exchange.					
36126824	9	42	theme	composite	1205:1213	arg1	gels					1215:1218	composite gels	1205:1218	composite gels with other biocompatible polymers	1205:1252	Using three solvents to prepare organogels can enable the encapsulation of various drugs and facilitate the preparation of composite gels with other biocompatible polymers.					
36126824	0	43	theme	forming	42:48	arg1	system					72:77	in situ forming implant drug delivery system	34:77	in situ forming implant drug delivery system	34:77	Self-assembled zein organogels as in situ forming implant drug delivery system and 3D printing ink.					
36126824	9	44	theme	gels	1215:1218	arg1	preparation					1190:1200	the preparation	1186:1200	the preparation of composite gels with other biocompatible polymers	1186:1252	Using three solvents to prepare organogels can enable the encapsulation of various drugs and facilitate the preparation of composite gels with other biocompatible polymers.					
36126824	0	45	dep	in	34:35	arg1	situ					37:40	situ	37:40	situ	37:40	Self-assembled zein organogels as in situ forming implant drug delivery system and 3D printing ink.					
36126824	6	46	theme	model	728:732	arg1	Ciprofloxacin					692:704	Ciprofloxacin	692:704	Ciprofloxacin	692:704	Ciprofloxacin was incorporated as a model drug and sustained release depending upon the solvent exchange rate was observed.					
36126824	6	46	theme	model	728:732	arg1	drug					734:737	a model drug	726:737	a model drug	726:737	Ciprofloxacin was incorporated as a model drug and sustained release depending upon the solvent exchange rate was observed.					
36126824	2	47	theme	Dimethyl	299:306	arg1	DMSO					319:322	DMSO	319:322	DMSO	319:322	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	2	47	theme	Dimethyl	299:306	arg1	sulfoxide					308:316	Dimethyl sulfoxide	299:316	Dimethyl sulfoxide (DMSO)	299:323	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	5	48	theme	implant	653:659	arg1	formation					661:669	mechanically robust in situ implant formation	625:669	mechanically robust in situ implant formation	625:669	Furthermore, water-induced self-assembly of zein allows mechanically robust in situ implant formation by solvent exchange.					
36126824	1	49	theme	biomedical	110:119	arg1	applications					121:132	biomedical applications	110:132	biomedical applications of organogels	110:146	Recently, biomedical applications of organogels have been increasing; however, there is a demand for bio-based polymers.					
36126824	3	50	theme	Parameters	463:472	arg1	component					432:440	the hydrogen bonding component	411:440	the hydrogen bonding component of Hansen Solubility Parameters	411:472	The gel formation was driven by the solvent's polarity and the hydrogen bonding component of Hansen Solubility Parameters was important in promoting gelation.					
36126824	3	50	theme	Parameters	463:472	arg1	important					478:486	important	478:486	important	478:486	The gel formation was driven by the solvent's polarity and the hydrogen bonding component of Hansen Solubility Parameters was important in promoting gelation.					
36126824	9	51	theme	other	1225:1229	arg1	polymers					1245:1252	other biocompatible polymers	1225:1252	other biocompatible polymers	1225:1252	Using three solvents to prepare organogels can enable the encapsulation of various drugs and facilitate the preparation of composite gels with other biocompatible polymers.					
36126824	7	52	from	implants	824:831	arg1	gel					844:846	agarose gel	836:846	agarose gel	836:846	In situ implants in agarose gel retained antibacterial efficacy against S. aureus for more than 14 days.					
36126824	1	53	theme	organogels	137:146	arg1	applications					121:132	biomedical applications	110:132	biomedical applications of organogels	110:146	Recently, biomedical applications of organogels have been increasing; however, there is a demand for bio-based polymers.					
36126824	1	54	theme	bio-based	201:209	arg1	polymers					211:218	bio-based polymers	201:218	bio-based polymers	201:218	Recently, biomedical applications of organogels have been increasing; however, there is a demand for bio-based polymers.					
36126824	0	55	theme	delivery	63:70	arg1	system					72:77	in situ forming implant drug delivery system	34:77	in situ forming implant drug delivery system	34:77	Self-assembled zein organogels as in situ forming implant drug delivery system and 3D printing ink.					
36126824	9	56	theme	biocompatible	1231:1243	arg1	polymers					1245:1252	other biocompatible polymers	1225:1252	other biocompatible polymers	1225:1252	Using three solvents to prepare organogels can enable the encapsulation of various drugs and facilitate the preparation of composite gels with other biocompatible polymers.					
36126824	8	57	theme	Zein-based	921:930	arg1	organogels					932:941	Zein-based organogels	921:941	Zein-based organogels	921:941	Zein-based organogels were further applied as 3D printing ink and it was found that zein gel in DMSO had superior printability than gels prepared in NMP and GF.					
36126824	8	57	theme	Zein-based	921:930	arg1	ink					979:981	3D printing ink	967:981	3D printing ink	967:981	Zein-based organogels were further applied as 3D printing ink and it was found that zein gel in DMSO had superior printability than gels prepared in NMP and GF.					
36126824	2	58	from	organogels	257:266	arg1	pyrrolidone					280:290	N-methyl pyrrolidone	271:290	N-methyl pyrrolidone (NMP)	271:296	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	2	58	from	organogels	257:266	arg1	NMP					293:295	NMP	293:295	NMP	293:295	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	2	58	from	organogels	257:266	arg1	DMSO					319:322	DMSO	319:322	DMSO	319:322	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	2	58	from	organogels	257:266	arg1	GF					347:348	GF	347:348	GF	347:348	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	2	58	from	organogels	257:266	arg1	sulfoxide					308:316	Dimethyl sulfoxide	299:316	Dimethyl sulfoxide (DMSO)	299:323	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	2	58	from	organogels	257:266	arg1	glycerol					330:337	glycerol formal	330:344	glycerol formal (GF)	330:349	Here, we report self-assembled zein organogels in N-methyl pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), and glycerol formal (GF).					
36126824	0	59	theme	drug	58:61	arg1	system					72:77	in situ forming implant drug delivery system	34:77	in situ forming implant drug delivery system	34:77	Self-assembled zein organogels as in situ forming implant drug delivery system and 3D printing ink.					
36126824	10	60	theme	drug	1325:1328	arg1	systems					1339:1345	3D printed drug delivery systems	1314:1345	3D printed drug delivery systems	1314:1345	These organogel systems can further be used for developing 3D printed drug delivery systems or scaffolds for tissue engineering.					
36126824	3	61	theme	Hansen	445:450	arg1	Parameters					463:472	Hansen Solubility Parameters	445:472	Hansen Solubility Parameters	445:472	The gel formation was driven by the solvent's polarity and the hydrogen bonding component of Hansen Solubility Parameters was important in promoting gelation.					
36126824	7	62	theme	antibacterial	857:869	arg1	efficacy					871:878	antibacterial efficacy	857:878	antibacterial efficacy against S. aureus for more than 14 days	857:918	In situ implants in agarose gel retained antibacterial efficacy against S. aureus for more than 14 days.					
37165777	8	0	used	used	744:747	arg2	samples					731:737	All samples	727:737	All samples	727:737	All samples were used for viral studies.					
37165777	7	1	theme	Stereotactic	642:653	arg1	biopsies					662:669	Stereotactic needle biopsies	642:669	Stereotactic needle biopsies of a lesion	642:681	Stereotactic needle biopsies of a lesion were performed; blood and CSF were sampled.					
37165777	4	2	theme	Post-treatment	454:467	arg1	monitoring					473:482	Post-treatment MRI monitoring	454:482	Post-treatment MRI monitoring	454:482	Post-treatment MRI monitoring revealed lesions around injection sites.					
37165777	13	3	theme	intracellular	1361:1373	arg1	oligodendrocytes					1294:1309	immature oligodendrocytes	1285:1309	immature oligodendrocytes	1285:1309	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	13	3	theme	intracellular	1361:1373	arg1	expression					1405:1414	strong intracellular and extracellular sulfamidase expression	1354:1414	strong intracellular and extracellular sulfamidase expression	1354:1414	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	9	4	dep	patient	876:882	arg1	the					872:874	the	872:874	the	872:874	Immunohistochemistry, electron microscopy, and transcriptome analysis were performed on brain tissue of the patient and various controls.					
37165777	3	5	theme	gene	304:307	arg1	trial					317:321	The AAVance gene therapy trial	292:321	The AAVance gene therapy trial	292:321	The AAVance gene therapy trial investigates AAVrh.10 overexpressing human sulfamidase (LYS-SAF302) delivered by intracerebral injection in children with MPSIIIA.					
37165777	17	6	theme	trial	1978:1982	arg1	results					1984:1990	The AAVance trial results	1966:1990	The AAVance trial results	1966:1990	The AAVance trial results will reveal the potential benefit-risk ratio of this therapy.					
37165777	3	7	with	children	431:438	arg1	MPSIIIA					445:451	MPSIIIA	445:451	MPSIIIA	445:451	The AAVance gene therapy trial investigates AAVrh.10 overexpressing human sulfamidase (LYS-SAF302) delivered by intracerebral injection in children with MPSIIIA.					
37165777	16	8	theme	cystic	1893:1898	arg1	degeneration					1913:1924	cystic white matter degeneration	1893:1924	cystic white matter degeneration at the site of highest gene expression	1893:1963	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
37165777	1	9	theme	neurocognitive	215:228	arg1	decline					230:236	neurocognitive decline	215:236	neurocognitive decline	215:236	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants results in sulfamidase deficiency, leading to neurocognitive decline and death.					
37165777	4	10	theme	injection	508:516	arg1	sites					518:522	injection sites	508:522	injection sites	508:522	Post-treatment MRI monitoring revealed lesions around injection sites.					
37165777	15	11	theme	local	1612:1616	arg1	overexpression					1630:1643	local sulfamidase overexpression	1612:1643	local sulfamidase overexpression	1612:1643	INTERPRETATION Results suggest that intracerebral gene therapy with local sulfamidase overexpression leads to dysfunction of transduced cells close to injection sites, with extracellular spilling of lysosomal enzymes.					
37165777	16	12	theme	matter	1906:1911	arg1	degeneration					1913:1924	cystic white matter degeneration	1893:1924	cystic white matter degeneration at the site of highest gene expression	1893:1963	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
37165777	16	13	from	site	1933:1936	arg1	degeneration					1913:1924	cystic white matter degeneration	1893:1924	cystic white matter degeneration at the site of highest gene expression	1893:1963	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
37165777	11	14	theme	transient	1111:1119	arg1	signs					1141:1145	transient slight neurological signs	1111:1145	transient slight neurological signs	1111:1145	The patient had transient slight neurological signs and is following near-normal development.					
37165777	9	15	theme	brain	856:860	arg1	tissue					862:867	brain tissue	856:867	brain tissue of the patient and various controls	856:903	Immunohistochemistry, electron microscopy, and transcriptome analysis were performed on brain tissue of the patient and various controls.					
37165777	16	16	theme	matrix	1788:1793	arg1	composition					1795:1805	extracellular matrix composition	1774:1805	extracellular matrix composition	1774:1805	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
37165777	11	17	contain	had	1107:1109	arg2	signs					1141:1145	transient slight neurological signs	1111:1145	transient slight neurological signs	1111:1145	The patient had transient slight neurological signs and is following near-normal development.					
37165777	11	17	contain	had	1107:1109	arg1	patient					1099:1105	The patient	1095:1105	The patient	1095:1105	The patient had transient slight neurological signs and is following near-normal development.					
37165777	10	18	theme	focal	927:931	arg1	lesions					933:939	focal lesions	927:939	focal lesions around injection sites	927:962	RESULTS MRI revealed focal lesions around injection sites with onset from 3 months after therapy, progression until 7 months post therapy with subsequent stabilization and some regression.					
37165777	13	19	theme	immature	1285:1292	arg1	oligodendrocytes					1294:1309	immature oligodendrocytes	1285:1309	immature oligodendrocytes	1285:1309	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	13	19	theme	immature	1285:1292	arg1	sulfate					1477:1483	hardly detectable intracellular or extracellular heparan sulfate	1420:1483	hardly detectable intracellular or extracellular heparan sulfate	1420:1483	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	13	19	theme	immature	1285:1292	arg1	expression					1405:1414	strong intracellular and extracellular sulfamidase expression	1354:1414	strong intracellular and extracellular sulfamidase expression	1354:1414	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	13	19	theme	immature	1285:1292	arg1	apoptosis					1343:1351	oligodendrocyte apoptosis	1327:1351	oligodendrocyte apoptosis	1327:1351	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	3	20	theme	human	360:364	arg1	LYS-SAF302					379:388	LYS-SAF302	379:388	LYS-SAF302	379:388	The AAVance gene therapy trial investigates AAVrh.10 overexpressing human sulfamidase (LYS-SAF302) delivered by intracerebral injection in children with MPSIIIA.					
37165777	3	20	theme	human	360:364	arg1	sulfamidase					366:376	human sulfamidase	360:376	human sulfamidase (LYS-SAF302) delivered by intracerebral injection in children with MPSIIIA	360:451	The AAVance gene therapy trial investigates AAVrh.10 overexpressing human sulfamidase (LYS-SAF302) delivered by intracerebral injection in children with MPSIIIA.					
37165777	9	21	theme	patient	876:882	arg1	tissue					862:867	brain tissue	856:867	brain tissue of the patient and various controls	856:903	Immunohistochemistry, electron microscopy, and transcriptome analysis were performed on brain tissue of the patient and various controls.					
37165777	16	22	theme	heparan	1817:1823	arg1	sulfate					1825:1831	heparan sulfate	1817:1831	heparan sulfate	1817:1831	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
37165777	15	23	theme	cells	1680:1684	arg1	dysfunction					1654:1664	dysfunction	1654:1664	dysfunction of transduced cells close to injection sites	1654:1709	INTERPRETATION Results suggest that intracerebral gene therapy with local sulfamidase overexpression leads to dysfunction of transduced cells close to injection sites, with extracellular spilling of lysosomal enzymes.					
37165777	11	24	theme	neurological	1128:1139	arg1	signs					1141:1145	transient slight neurological signs	1111:1145	transient slight neurological signs	1111:1145	The patient had transient slight neurological signs and is following near-normal development.					
37165777	9	25	theme	various	888:894	arg1	controls					896:903	various controls	888:903	various controls	888:903	Immunohistochemistry, electron microscopy, and transcriptome analysis were performed on brain tissue of the patient and various controls.					
37165777	3	26	from	injection	418:426	arg1	children					431:438	children	431:438	children with MPSIIIA	431:451	The AAVance gene therapy trial investigates AAVrh.10 overexpressing human sulfamidase (LYS-SAF302) delivered by intracerebral injection in children with MPSIIIA.					
37165777	7	27	theme	lesion	676:681	arg1	biopsies					662:669	Stereotactic needle biopsies	642:669	Stereotactic needle biopsies of a lesion	642:681	Stereotactic needle biopsies of a lesion were performed; blood and CSF were sampled.					
37165777	1	28	theme	recessive	145:153	arg1	variants					160:167	recessive SGSH variants	145:167	recessive SGSH variants	145:167	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants results in sulfamidase deficiency, leading to neurocognitive decline and death.					
37165777	1	29	theme	Mucopolysaccharidosis	93:113	arg1	IIIA					120:123	OBJECTIVE Mucopolysaccharidosis type IIIA	83:123	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants	83:167	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants results in sulfamidase deficiency, leading to neurocognitive decline and death.					
37165777	1	29	theme	Mucopolysaccharidosis	93:113	arg1	MPSIIIA					126:132	MPSIIIA	126:132	MPSIIIA	126:132	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants results in sulfamidase deficiency, leading to neurocognitive decline and death.					
37165777	0	30	theme	Focal	0:4	arg1	lesions					6:12	Focal lesions	0:12	Focal lesions	0:12	Focal lesions following intracerebral gene therapy for mucopolysaccharidosis IIIA.					
37165777	7	31	theme	needle	655:660	arg1	biopsies					662:669	Stereotactic needle biopsies	642:669	Stereotactic needle biopsies of a lesion	642:681	Stereotactic needle biopsies of a lesion were performed; blood and CSF were sampled.					
37165777	14	32	theme	protein	1516:1522	arg1	response					1524:1531	the unfolded protein response	1503:1531	the unfolded protein response	1503:1531	No activation of the unfolded protein response was found.					
37165777	10	33	with	therapy	1036:1042	arg1	stabilization					1060:1072	subsequent stabilization	1049:1072	subsequent stabilization	1049:1072	RESULTS MRI revealed focal lesions around injection sites with onset from 3 months after therapy, progression until 7 months post therapy with subsequent stabilization and some regression.					
37165777	17	34	theme	therapy	2045:2051	arg1	ratio					2031:2035	the potential benefit-risk ratio	2004:2035	the potential benefit-risk ratio of this therapy	2004:2051	The AAVance trial results will reveal the potential benefit-risk ratio of this therapy.					
37165777	13	35	theme	heparan	1469:1475	arg1	oligodendrocytes					1294:1309	immature oligodendrocytes	1285:1309	immature oligodendrocytes	1285:1309	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	13	35	theme	heparan	1469:1475	arg1	sulfate					1477:1483	hardly detectable intracellular or extracellular heparan sulfate	1420:1483	hardly detectable intracellular or extracellular heparan sulfate	1420:1483	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	12	36	theme	viral	1204:1208	arg1	cause					1240:1244	viral or immunological/inflammatory cause	1204:1244	viral or immunological/inflammatory cause	1204:1244	No evidence of viral or immunological/inflammatory cause was found.					
37165777	0	37	theme	gene	38:41	arg1	therapy					43:49	intracerebral gene therapy	24:49	intracerebral gene therapy for mucopolysaccharidosis IIIA	24:80	Focal lesions following intracerebral gene therapy for mucopolysaccharidosis IIIA.					
37165777	10	38	theme	subsequent	1049:1058	arg1	stabilization					1060:1072	subsequent stabilization	1049:1072	subsequent stabilization	1049:1072	RESULTS MRI revealed focal lesions around injection sites with onset from 3 months after therapy, progression until 7 months post therapy with subsequent stabilization and some regression.					
37165777	15	39	theme	enzymes	1753:1759	arg1	spilling					1731:1738	extracellular spilling	1717:1738	extracellular spilling of lysosomal enzymes	1717:1759	INTERPRETATION Results suggest that intracerebral gene therapy with local sulfamidase overexpression leads to dysfunction of transduced cells close to injection sites, with extracellular spilling of lysosomal enzymes.					
37165777	15	40	theme	intracerebral	1580:1592	arg1	therapy					1599:1605	intracerebral gene therapy	1580:1605	intracerebral gene therapy with local sulfamidase overexpression	1580:1643	INTERPRETATION Results suggest that intracerebral gene therapy with local sulfamidase overexpression leads to dysfunction of transduced cells close to injection sites, with extracellular spilling of lysosomal enzymes.					
37165777	12	41	theme	immunological/inflammatory	1213:1238	arg1	cause					1240:1244	viral or immunological/inflammatory cause	1204:1244	viral or immunological/inflammatory cause	1204:1244	No evidence of viral or immunological/inflammatory cause was found.					
37165777	13	42	theme	detectable	1427:1436	arg1	oligodendrocytes					1294:1309	immature oligodendrocytes	1285:1309	immature oligodendrocytes	1285:1309	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	13	42	theme	detectable	1427:1436	arg1	sulfate					1477:1483	hardly detectable intracellular or extracellular heparan sulfate	1420:1483	hardly detectable intracellular or extracellular heparan sulfate	1420:1483	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	10	43	from	3 months	980:987	arg1	regression					1083:1092	some regression	1078:1092	some regression	1078:1092	RESULTS MRI revealed focal lesions around injection sites with onset from 3 months after therapy, progression until 7 months post therapy with subsequent stabilization and some regression.					
37165777	10	43	from	3 months	980:987	arg1	onset					969:973	onset	969:973	onset from 3 months after therapy	969:1001	RESULTS MRI revealed focal lesions around injection sites with onset from 3 months after therapy, progression until 7 months post therapy with subsequent stabilization and some regression.					
37165777	10	43	from	3 months	980:987	arg1	progression					1004:1014	progression	1004:1014	progression until 7 months post therapy with subsequent stabilization	1004:1072	RESULTS MRI revealed focal lesions around injection sites with onset from 3 months after therapy, progression until 7 months post therapy with subsequent stabilization and some regression.					
37165777	16	44	theme	highest	1941:1947	arg1	expression					1954:1963	highest gene expression	1941:1963	highest gene expression	1941:1963	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
37165777	17	45	theme	potential	2008:2016	arg1	ratio					2031:2035	the potential benefit-risk ratio	2004:2035	the potential benefit-risk ratio of this therapy	2004:2051	The AAVance trial results will reveal the potential benefit-risk ratio of this therapy.					
37165777	15	46	theme	extracellular	1717:1729	arg1	spilling					1731:1738	extracellular spilling	1717:1738	extracellular spilling of lysosomal enzymes	1717:1759	INTERPRETATION Results suggest that intracerebral gene therapy with local sulfamidase overexpression leads to dysfunction of transduced cells close to injection sites, with extracellular spilling of lysosomal enzymes.					
37165777	15	47	theme	INTERPRETATION	1544:1557	arg1	Results					1559:1565	INTERPRETATION Results	1544:1565	INTERPRETATION Results	1544:1565	INTERPRETATION Results suggest that intracerebral gene therapy with local sulfamidase overexpression leads to dysfunction of transduced cells close to injection sites, with extracellular spilling of lysosomal enzymes.					
37165777	16	48	theme	expression	1954:1963	arg1	expression					1954:1963	highest gene expression	1941:1963	highest gene expression	1941:1963	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
37165777	16	48	theme	expression	1954:1963	arg1	site					1933:1936	the site	1929:1936	the site of highest gene expression	1929:1963	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
37165777	12	49	theme	cause	1240:1244	arg1	evidence					1192:1199	No evidence	1189:1199	No evidence of viral or immunological/inflammatory cause	1189:1244	No evidence of viral or immunological/inflammatory cause was found.					
37165777	6	50	theme	MRI	615:617	arg1	details					619:625	MRI details	615:625	MRI details	615:625	METHODS Clinical and MRI details were reviewed.					
37165777	13	51	theme	sulfamidase	1393:1403	arg1	oligodendrocytes					1294:1309	immature oligodendrocytes	1285:1309	immature oligodendrocytes	1285:1309	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	13	51	theme	sulfamidase	1393:1403	arg1	expression					1405:1414	strong intracellular and extracellular sulfamidase expression	1354:1414	strong intracellular and extracellular sulfamidase expression	1354:1414	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	6	52	theme	Clinical	602:609	arg1	METHODS					594:600	METHODS	594:600	METHODS Clinical	594:609	METHODS Clinical and MRI details were reviewed.					
37165777	4	53	theme	MRI	469:471	arg1	monitoring					473:482	Post-treatment MRI monitoring	454:482	Post-treatment MRI monitoring	454:482	Post-treatment MRI monitoring revealed lesions around injection sites.					
37165777	13	54	theme	strong	1354:1359	arg1	oligodendrocytes					1294:1309	immature oligodendrocytes	1285:1309	immature oligodendrocytes	1285:1309	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	13	54	theme	strong	1354:1359	arg1	expression					1405:1414	strong intracellular and extracellular sulfamidase expression	1354:1414	strong intracellular and extracellular sulfamidase expression	1354:1414	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	1	55	theme	sulfamidase	180:190	arg1	deficiency					192:201	sulfamidase deficiency	180:201	sulfamidase deficiency	180:201	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants results in sulfamidase deficiency, leading to neurocognitive decline and death.					
37165777	9	56	theme	electron	790:797	arg1	microscopy					799:808	electron microscopy	790:808	electron microscopy	790:808	Immunohistochemistry, electron microscopy, and transcriptome analysis were performed on brain tissue of the patient and various controls.					
37165777	8	57	theme	viral	753:757	arg1	studies					759:765	viral studies	753:765	viral studies	753:765	All samples were used for viral studies.					
37165777	17	58	theme	AAVance	1970:1976	arg1	results					1984:1990	The AAVance trial results	1966:1990	The AAVance trial results	1966:1990	The AAVance trial results will reveal the potential benefit-risk ratio of this therapy.					
37165777	3	59	theme	therapy	309:315	arg1	trial					317:321	The AAVance gene therapy trial	292:321	The AAVance gene therapy trial	292:321	The AAVance gene therapy trial investigates AAVrh.10 overexpressing human sulfamidase (LYS-SAF302) delivered by intracerebral injection in children with MPSIIIA.					
37165777	9	60	theme	transcriptome	815:827	arg1	analysis					829:836	transcriptome analysis	815:836	transcriptome analysis	815:836	Immunohistochemistry, electron microscopy, and transcriptome analysis were performed on brain tissue of the patient and various controls.					
37165777	2	61	theme	disease-modifying	252:268	arg1	therapy					270:276	No disease-modifying therapy	249:276	No disease-modifying therapy	249:276	No disease-modifying therapy is available.					
37165777	10	62	theme	RESULTS	906:912	arg1	MRI					914:916	RESULTS MRI	906:916	RESULTS MRI	906:916	RESULTS MRI revealed focal lesions around injection sites with onset from 3 months after therapy, progression until 7 months post therapy with subsequent stabilization and some regression.					
37165777	3	63	theme	AAVance	296:302	arg1	trial					317:321	The AAVance gene therapy trial	292:321	The AAVance gene therapy trial	292:321	The AAVance gene therapy trial investigates AAVrh.10 overexpressing human sulfamidase (LYS-SAF302) delivered by intracerebral injection in children with MPSIIIA.					
37165777	16	64	theme	extracellular	1774:1786	arg1	composition					1795:1805	extracellular matrix composition	1774:1805	extracellular matrix composition	1774:1805	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
37165777	15	65	theme	sulfamidase	1618:1628	arg1	overexpression					1630:1643	local sulfamidase overexpression	1612:1643	local sulfamidase overexpression	1612:1643	INTERPRETATION Results suggest that intracerebral gene therapy with local sulfamidase overexpression leads to dysfunction of transduced cells close to injection sites, with extracellular spilling of lysosomal enzymes.					
37165777	16	66	theme	white	1900:1904	arg1	degeneration					1913:1924	cystic white matter degeneration	1893:1924	cystic white matter degeneration at the site of highest gene expression	1893:1963	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
37165777	10	67	theme	injection	948:956	arg1	sites					958:962	injection sites	948:962	injection sites	948:962	RESULTS MRI revealed focal lesions around injection sites with onset from 3 months after therapy, progression until 7 months post therapy with subsequent stabilization and some regression.					
37165777	13	68	theme	oligodendrocyte	1327:1341	arg1	oligodendrocytes					1294:1309	immature oligodendrocytes	1285:1309	immature oligodendrocytes	1285:1309	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	13	68	theme	oligodendrocyte	1327:1341	arg1	apoptosis					1343:1351	oligodendrocyte apoptosis	1327:1351	oligodendrocyte apoptosis	1327:1351	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	1	69	theme	SGSH	155:158	arg1	variants					160:167	recessive SGSH variants	145:167	recessive SGSH variants	145:167	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants results in sulfamidase deficiency, leading to neurocognitive decline and death.					
37165777	15	70	theme	injection	1695:1703	arg1	sites					1705:1709	injection sites	1695:1709	injection sites	1695:1709	INTERPRETATION Results suggest that intracerebral gene therapy with local sulfamidase overexpression leads to dysfunction of transduced cells close to injection sites, with extracellular spilling of lysosomal enzymes.					
37165777	11	71	theme	slight	1121:1126	arg1	signs					1141:1145	transient slight neurological signs	1111:1145	transient slight neurological signs	1111:1145	The patient had transient slight neurological signs and is following near-normal development.					
37165777	15	72	with	therapy	1599:1605	arg1	overexpression					1630:1643	local sulfamidase overexpression	1612:1643	local sulfamidase overexpression	1612:1643	INTERPRETATION Results suggest that intracerebral gene therapy with local sulfamidase overexpression leads to dysfunction of transduced cells close to injection sites, with extracellular spilling of lysosomal enzymes.					
37165777	16	73	theme	astrocyte	1842:1850	arg1	function					1872:1879	astrocyte and oligodendrocyte function	1842:1879	function	1872:1879	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
37165777	3	74	theme	intracerebral	404:416	arg1	injection					418:426	intracerebral injection	404:426	intracerebral injection in children with MPSIIIA	404:451	The AAVance gene therapy trial investigates AAVrh.10 overexpressing human sulfamidase (LYS-SAF302) delivered by intracerebral injection in children with MPSIIIA.					
37165777	9	75	theme	controls	896:903	arg1	tissue					862:867	brain tissue	856:867	brain tissue of the patient and various controls	856:903	Immunohistochemistry, electron microscopy, and transcriptome analysis were performed on brain tissue of the patient and various controls.					
37165777	15	76	theme	transduced	1669:1678	arg1	cells					1680:1684	transduced cells	1669:1684	transduced cells close to injection sites	1669:1709	INTERPRETATION Results suggest that intracerebral gene therapy with local sulfamidase overexpression leads to dysfunction of transduced cells close to injection sites, with extracellular spilling of lysosomal enzymes.					
37165777	16	77	theme	oligodendrocyte	1856:1870	arg1	function					1872:1879	astrocyte and oligodendrocyte function	1842:1879	function	1872:1879	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
37165777	1	78	theme	OBJECTIVE	83:91	arg1	IIIA					120:123	OBJECTIVE Mucopolysaccharidosis type IIIA	83:123	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants	83:167	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants results in sulfamidase deficiency, leading to neurocognitive decline and death.					
37165777	1	78	theme	OBJECTIVE	83:91	arg1	MPSIIIA					126:132	MPSIIIA	126:132	MPSIIIA	126:132	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants results in sulfamidase deficiency, leading to neurocognitive decline and death.					
37165777	1	79	theme	type	115:118	arg1	IIIA					120:123	OBJECTIVE Mucopolysaccharidosis type IIIA	83:123	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants	83:167	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants results in sulfamidase deficiency, leading to neurocognitive decline and death.					
37165777	1	79	theme	type	115:118	arg1	MPSIIIA					126:132	MPSIIIA	126:132	MPSIIIA	126:132	OBJECTIVE Mucopolysaccharidosis type IIIA (MPSIIIA) caused by recessive SGSH variants results in sulfamidase deficiency, leading to neurocognitive decline and death.					
37165777	14	80	theme	response	1524:1531	arg1	activation					1489:1498	No activation	1486:1498	No activation of the unfolded protein response	1486:1531	No activation of the unfolded protein response was found.					
37165777	0	81	theme	intracerebral	24:36	arg1	therapy					43:49	intracerebral gene therapy	24:49	intracerebral gene therapy for mucopolysaccharidosis IIIA	24:80	Focal lesions following intracerebral gene therapy for mucopolysaccharidosis IIIA.					
37165777	14	82	theme	unfolded	1507:1514	arg1	response					1524:1531	the unfolded protein response	1503:1531	the unfolded protein response	1503:1531	No activation of the unfolded protein response was found.					
37165777	13	83	theme	extracellular	1455:1467	arg1	oligodendrocytes					1294:1309	immature oligodendrocytes	1285:1309	immature oligodendrocytes	1285:1309	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	13	83	theme	extracellular	1455:1467	arg1	sulfate					1477:1483	hardly detectable intracellular or extracellular heparan sulfate	1420:1483	hardly detectable intracellular or extracellular heparan sulfate	1420:1483	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	11	84	theme	near-normal	1164:1174	arg1	development					1176:1186	near-normal development	1164:1186	near-normal development	1164:1186	The patient had transient slight neurological signs and is following near-normal development.					
37165777	15	85	theme	lysosomal	1743:1751	arg1	enzymes					1753:1759	lysosomal enzymes	1743:1759	lysosomal enzymes	1743:1759	INTERPRETATION Results suggest that intracerebral gene therapy with local sulfamidase overexpression leads to dysfunction of transduced cells close to injection sites, with extracellular spilling of lysosomal enzymes.					
37165777	0	86	theme	mucopolysaccharidosis	55:75	arg1	IIIA					77:80	mucopolysaccharidosis IIIA	55:80	mucopolysaccharidosis IIIA	55:80	Focal lesions following intracerebral gene therapy for mucopolysaccharidosis IIIA.					
37165777	15	87	theme	gene	1594:1597	arg1	therapy					1599:1605	intracerebral gene therapy	1580:1605	intracerebral gene therapy with local sulfamidase overexpression	1580:1643	INTERPRETATION Results suggest that intracerebral gene therapy with local sulfamidase overexpression leads to dysfunction of transduced cells close to injection sites, with extracellular spilling of lysosomal enzymes.					
37165777	13	88	theme	intracellular	1438:1450	arg1	oligodendrocytes					1294:1309	immature oligodendrocytes	1285:1309	immature oligodendrocytes	1285:1309	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	13	88	theme	intracellular	1438:1450	arg1	sulfate					1477:1483	hardly detectable intracellular or extracellular heparan sulfate	1420:1483	hardly detectable intracellular or extracellular heparan sulfate	1420:1483	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	17	89	theme	benefit-risk	2018:2029	arg1	ratio					2031:2035	the potential benefit-risk ratio	2004:2035	the potential benefit-risk ratio of this therapy	2004:2051	The AAVance trial results will reveal the potential benefit-risk ratio of this therapy.					
37165777	13	90	theme	extracellular	1379:1391	arg1	oligodendrocytes					1294:1309	immature oligodendrocytes	1285:1309	immature oligodendrocytes	1285:1309	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	13	90	theme	extracellular	1379:1391	arg1	expression					1405:1414	strong intracellular and extracellular sulfamidase expression	1354:1414	strong intracellular and extracellular sulfamidase expression	1354:1414	Immunohistochemistry showed immature oligodendrocytes and astrocytes, oligodendrocyte apoptosis, strong intracellular and extracellular sulfamidase expression and hardly detectable intracellular or extracellular heparan sulfate.					
37165777	16	91	theme	gene	1949:1952	arg1	expression					1954:1963	highest gene expression	1941:1963	highest gene expression	1941:1963	This alters extracellular matrix composition, depletes heparan sulfate, impairs astrocyte and oligodendrocyte function, and causes cystic white matter degeneration at the site of highest gene expression.					
36108445	0	0	theme	bean	93:96	arg1	sprouts					98:104	mung bean sprouts	88:104	mung bean sprouts	88:104	Sucrose-induced abiotic stress improves the phytochemical profiles and bioactivities of mung bean sprouts.					
36108445	7	1	theme	effective	982:990	arg1	strategy					992:999	an effective strategy	979:999	an effective strategy to improve the dietary phytochemical composition and provide potential health benefits	979:1086	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	7	1	theme	effective	982:990	arg1	application					913:923	the elicitor application	900:923	the elicitor application of sucrose at 3 % during mung bean sprouting	900:968	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	1	2	theme	sucrose	149:155	arg1	treatment					157:165	sucrose treatment	149:165	sucrose treatment	149:165	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	7	3	theme	elicitor	904:911	arg1	strategy					992:999	an effective strategy	979:999	an effective strategy to improve the dietary phytochemical composition and provide potential health benefits	979:1086	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	7	3	theme	elicitor	904:911	arg1	application					913:923	the elicitor application	900:923	the elicitor application of sucrose at 3 % during mung bean sprouting	900:968	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	4	4	theme	γ-aminobutyric	569:582	arg1	acid					584:587	γ-aminobutyric acid	569:587	γ-aminobutyric acid	569:587	However, the sucrose-treated sprouts contained more polyphenols, flavonoids, γ-aminobutyric acid, phytosterols, and vitamins.					
36108445	0	5	theme	mung	88:91	arg1	bean					93:96	mung bean	88:96	mung bean sprouts	88:104	Sucrose-induced abiotic stress improves the phytochemical profiles and bioactivities of mung bean sprouts.					
36108445	1	6	theme	treatment	157:165	arg1	impact					139:144	the impact	135:144	the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts	135:267	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	1	7	theme	mung	251:254	arg1	bean					256:259	mung bean	251:259	mung bean sprouts	251:267	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	6	8	theme	insulin	812:818	arg1	resistance					820:829	markedly improved FFA-induced insulin resistance	782:829	markedly improved FFA-induced insulin resistance	782:829	The sprouts treated with 2-3 % sucrose showed markedly improved FFA-induced insulin resistance and alcohol-induced oxidative injury in HepG2 cells.					
36108445	1	9	theme	bean	256:259	arg1	sprouts					261:267	mung bean sprouts	251:267	mung bean sprouts	251:267	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	0	10	theme	sprouts	98:104	arg1	bioactivities					71:83	bioactivities	71:83	bioactivities	71:83	Sucrose-induced abiotic stress improves the phytochemical profiles and bioactivities of mung bean sprouts.					
36108445	0	10	theme	sprouts	98:104	arg1	profiles					58:65	the phytochemical profiles	40:65	the phytochemical profiles	40:65	Sucrose-induced abiotic stress improves the phytochemical profiles and bioactivities of mung bean sprouts.					
36108445	4	11	contain	contained	529:537	arg2	vitamins					608:615	vitamins	608:615	vitamins	608:615	However, the sucrose-treated sprouts contained more polyphenols, flavonoids, γ-aminobutyric acid, phytosterols, and vitamins.					
36108445	4	11	contain	contained	529:537	arg2	flavonoids					557:566	flavonoids	557:566	flavonoids	557:566	However, the sucrose-treated sprouts contained more polyphenols, flavonoids, γ-aminobutyric acid, phytosterols, and vitamins.					
36108445	4	11	contain	contained	529:537	arg2	more					539:542	more	539:542	more	539:542	However, the sucrose-treated sprouts contained more polyphenols, flavonoids, γ-aminobutyric acid, phytosterols, and vitamins.					
36108445	4	11	contain	contained	529:537	arg2	acid					584:587	γ-aminobutyric acid	569:587	γ-aminobutyric acid	569:587	However, the sucrose-treated sprouts contained more polyphenols, flavonoids, γ-aminobutyric acid, phytosterols, and vitamins.					
36108445	4	11	contain	contained	529:537	arg1	sprouts					521:527	the sucrose-treated sprouts	501:527	the sucrose-treated sprouts	501:527	However, the sucrose-treated sprouts contained more polyphenols, flavonoids, γ-aminobutyric acid, phytosterols, and vitamins.					
36108445	4	11	contain	contained	529:537	arg2	phytosterols					590:601	phytosterols	590:601	phytosterols	590:601	However, the sucrose-treated sprouts contained more polyphenols, flavonoids, γ-aminobutyric acid, phytosterols, and vitamins.					
36108445	1	12	from	impact	139:144	arg1	activities					237:246	biological activities	226:246	biological activities	226:246	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	1	12	from	impact	139:144	arg1	conditions					181:190	the growth conditions	170:190	the growth conditions	170:190	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	1	12	from	impact	139:144	arg1	quality					213:219	dietary nutritional quality	193:219	dietary nutritional quality	193:219	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	2	13	theme	bean	275:278	arg1	seeds					280:284	Mung bean seeds	270:284	Mung bean seeds	270:284	Mung bean seeds were sprouted with solutions containing different sucrose concentrations (10, 20, and 30 g/L).					
36108445	1	14	theme	sprouts	261:267	arg1	conditions					181:190	the growth conditions	170:190	the growth conditions	170:190	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	1	14	theme	sprouts	261:267	arg1	activities					237:246	biological activities	226:246	biological activities	226:246	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	1	14	theme	sprouts	261:267	arg1	quality					213:219	dietary nutritional quality	193:219	dietary nutritional quality	193:219	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	3	15	theme	fresh	457:461	arg1	weight					463:468	fresh weight	457:468	fresh weight	457:468	The application of exogenous sucrose significantly decreased the height and fresh weight of mung bean sprouts.					
36108445	2	16	theme	Mung	270:273	arg1	bean					275:278	Mung bean	270:278	Mung bean seeds	270:284	Mung bean seeds were sprouted with solutions containing different sucrose concentrations (10, 20, and 30 g/L).					
36108445	3	17	theme	exogenous	400:408	arg1	sucrose					410:416	exogenous sucrose	400:416	exogenous sucrose	400:416	The application of exogenous sucrose significantly decreased the height and fresh weight of mung bean sprouts.					
36108445	7	18	theme	dietary	1016:1022	arg1	composition					1038:1048	the dietary phytochemical composition	1012:1048	the dietary phytochemical composition	1012:1048	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	6	19	theme	improved	791:798	arg1	resistance					820:829	markedly improved FFA-induced insulin resistance	782:829	markedly improved FFA-induced insulin resistance	782:829	The sprouts treated with 2-3 % sucrose showed markedly improved FFA-induced insulin resistance and alcohol-induced oxidative injury in HepG2 cells.					
36108445	0	20	theme	abiotic	16:22	arg1	stress					24:29	Sucrose-induced abiotic stress	0:29	Sucrose-induced abiotic stress	0:29	Sucrose-induced abiotic stress improves the phytochemical profiles and bioactivities of mung bean sprouts.					
36108445	4	21	dep	more	539:542	arg1	polyphenols					544:554	polyphenols	544:554	polyphenols	544:554	However, the sucrose-treated sprouts contained more polyphenols, flavonoids, γ-aminobutyric acid, phytosterols, and vitamins.					
36108445	5	22	theme	antioxidant	622:632	arg1	capacities					634:643	The antioxidant capacities	618:643	The antioxidant capacities	618:643	The antioxidant capacities were also significantly higher in the sucrose-treated sprouts than in the control sprouts.					
36108445	5	22	theme	antioxidant	622:632	arg1	higher					669:674	higher	669:674	higher	669:674	The antioxidant capacities were also significantly higher in the sucrose-treated sprouts than in the control sprouts.					
36108445	7	23	theme	mung	950:953	arg1	bean					955:958	mung bean	950:958	mung bean sprouting	950:968	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	7	24	from	%	941:941	arg1	strategy					992:999	an effective strategy	979:999	an effective strategy to improve the dietary phytochemical composition and provide potential health benefits	979:1086	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	7	24	from	%	941:941	arg1	application					913:923	the elicitor application	900:923	the elicitor application of sucrose at 3 % during mung bean sprouting	900:968	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	1	25	theme	growth	174:179	arg1	conditions					181:190	the growth conditions	170:190	the growth conditions	170:190	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	0	26	theme	Sucrose-induced	0:14	arg1	stress					24:29	Sucrose-induced abiotic stress	0:29	Sucrose-induced abiotic stress	0:29	Sucrose-induced abiotic stress improves the phytochemical profiles and bioactivities of mung bean sprouts.					
36108445	3	27	theme	sucrose	410:416	arg1	application					385:395	The application	381:395	The application of exogenous sucrose	381:416	The application of exogenous sucrose significantly decreased the height and fresh weight of mung bean sprouts.					
36108445	7	28	theme	bean	955:958	arg1	sprouting					960:968	mung bean sprouting	950:968	mung bean sprouting	950:968	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	6	29	theme	FFA-induced	800:810	arg1	resistance					820:829	markedly improved FFA-induced insulin resistance	782:829	markedly improved FFA-induced insulin resistance	782:829	The sprouts treated with 2-3 % sucrose showed markedly improved FFA-induced insulin resistance and alcohol-induced oxidative injury in HepG2 cells.					
36108445	2	30	dep	concentrations	344:357	arg1	30 g/L					372:377	30 g/L	372:377	30 g/L	372:377	Mung bean seeds were sprouted with solutions containing different sucrose concentrations (10, 20, and 30 g/L).					
36108445	2	30	dep	concentrations	344:357	arg1	10					360:361	10	360:361	10	360:361	Mung bean seeds were sprouted with solutions containing different sucrose concentrations (10, 20, and 30 g/L).					
36108445	2	30	dep	concentrations	344:357	arg1	20					364:365	20	364:365	20	364:365	Mung bean seeds were sprouted with solutions containing different sucrose concentrations (10, 20, and 30 g/L).					
36108445	7	31	theme	phytochemical	1024:1036	arg1	composition					1038:1048	the dietary phytochemical composition	1012:1048	the dietary phytochemical composition	1012:1048	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	1	32	theme	dietary	193:199	arg1	quality					213:219	dietary nutritional quality	193:219	dietary nutritional quality	193:219	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	0	33	theme	phytochemical	44:56	arg1	profiles					58:65	the phytochemical profiles	40:65	the phytochemical profiles	40:65	Sucrose-induced abiotic stress improves the phytochemical profiles and bioactivities of mung bean sprouts.					
36108445	3	34	theme	bean	478:481	arg1	sprouts					483:489	mung bean sprouts	473:489	mung bean sprouts	473:489	The application of exogenous sucrose significantly decreased the height and fresh weight of mung bean sprouts.					
36108445	7	35	theme	health	1072:1077	arg1	benefits					1079:1086	potential health benefits	1062:1086	potential health benefits	1062:1086	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	6	36	from	resistance	820:829	arg1	cells					877:881	HepG2 cells	871:881	HepG2 cells	871:881	The sprouts treated with 2-3 % sucrose showed markedly improved FFA-induced insulin resistance and alcohol-induced oxidative injury in HepG2 cells.					
36108445	1	37	theme	nutritional	201:211	arg1	quality					213:219	dietary nutritional quality	193:219	dietary nutritional quality	193:219	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	4	38	theme	sucrose-treated	505:519	arg1	sprouts					521:527	the sucrose-treated sprouts	501:527	the sucrose-treated sprouts	501:527	However, the sucrose-treated sprouts contained more polyphenols, flavonoids, γ-aminobutyric acid, phytosterols, and vitamins.					
36108445	5	39	theme	control	719:725	arg1	sprouts					727:733	the control sprouts	715:733	the control sprouts	715:733	The antioxidant capacities were also significantly higher in the sucrose-treated sprouts than in the control sprouts.					
36108445	6	40	theme	%	765:765	arg1	sucrose					767:773	2-3 % sucrose	761:773	2-3 % sucrose	761:773	The sprouts treated with 2-3 % sucrose showed markedly improved FFA-induced insulin resistance and alcohol-induced oxidative injury in HepG2 cells.					
36108445	3	41	theme	sprouts	483:489	arg1	height					446:451	height	446:451	height	446:451	The application of exogenous sucrose significantly decreased the height and fresh weight of mung bean sprouts.					
36108445	3	41	theme	sprouts	483:489	arg1	weight					463:468	fresh weight	457:468	fresh weight	457:468	The application of exogenous sucrose significantly decreased the height and fresh weight of mung bean sprouts.					
36108445	6	42	theme	HepG2	871:875	arg1	cells					877:881	HepG2 cells	871:881	HepG2 cells	871:881	The sprouts treated with 2-3 % sucrose showed markedly improved FFA-induced insulin resistance and alcohol-induced oxidative injury in HepG2 cells.					
36108445	6	43	theme	2-3 	761:764	arg1	%					765:765	%	765:765	%	765:765	The sprouts treated with 2-3 % sucrose showed markedly improved FFA-induced insulin resistance and alcohol-induced oxidative injury in HepG2 cells.					
36108445	3	44	theme	mung	473:476	arg1	bean					478:481	mung bean	473:481	mung bean sprouts	473:489	The application of exogenous sucrose significantly decreased the height and fresh weight of mung bean sprouts.					
36108445	6	45	from	injury	861:866	arg1	cells					877:881	HepG2 cells	871:881	HepG2 cells	871:881	The sprouts treated with 2-3 % sucrose showed markedly improved FFA-induced insulin resistance and alcohol-induced oxidative injury in HepG2 cells.					
36108445	7	46	theme	potential	1062:1070	arg1	benefits					1079:1086	potential health benefits	1062:1086	potential health benefits	1062:1086	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	2	47	theme	sucrose	336:342	arg1	concentrations					344:357	different sucrose concentrations	326:357	different sucrose concentrations (10, 20, and 30 g/L)	326:378	Mung bean seeds were sprouted with solutions containing different sucrose concentrations (10, 20, and 30 g/L).					
36108445	7	48	theme	sucrose	928:934	arg1	strategy					992:999	an effective strategy	979:999	an effective strategy to improve the dietary phytochemical composition and provide potential health benefits	979:1086	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	7	48	theme	sucrose	928:934	arg1	application					913:923	the elicitor application	900:923	the elicitor application of sucrose at 3 % during mung bean sprouting	900:968	Taken together, the elicitor application of sucrose at 3 % during mung bean sprouting could be an effective strategy to improve the dietary phytochemical composition and provide potential health benefits.					
36108445	5	49	theme	sucrose-treated	683:697	arg1	sprouts					699:705	the sucrose-treated sprouts	679:705	the sucrose-treated sprouts	679:705	The antioxidant capacities were also significantly higher in the sucrose-treated sprouts than in the control sprouts.					
36108445	2	50	theme	different	326:334	arg1	concentrations					344:357	different sucrose concentrations	326:357	different sucrose concentrations (10, 20, and 30 g/L)	326:378	Mung bean seeds were sprouted with solutions containing different sucrose concentrations (10, 20, and 30 g/L).					
36108445	6	51	theme	oxidative	851:859	arg1	injury					861:866	alcohol-induced oxidative injury	835:866	alcohol-induced oxidative injury	835:866	The sprouts treated with 2-3 % sucrose showed markedly improved FFA-induced insulin resistance and alcohol-induced oxidative injury in HepG2 cells.					
36108445	3	52	dep	height	446:451	arg1	the					442:444	the	442:444	the	442:444	The application of exogenous sucrose significantly decreased the height and fresh weight of mung bean sprouts.					
36108445	6	53	theme	alcohol-induced	835:849	arg1	injury					861:866	alcohol-induced oxidative injury	835:866	alcohol-induced oxidative injury	835:866	The sprouts treated with 2-3 % sucrose showed markedly improved FFA-induced insulin resistance and alcohol-induced oxidative injury in HepG2 cells.					
36108445	1	54	theme	biological	226:235	arg1	activities					237:246	biological activities	226:246	biological activities	226:246	This study aimed to examine the impact of sucrose treatment on the growth conditions, dietary nutritional quality, and biological activities of mung bean sprouts.					
36108445	2	55	contain	containing	315:324	arg2	concentrations					344:357	different sucrose concentrations	326:357	different sucrose concentrations (10, 20, and 30 g/L)	326:378	Mung bean seeds were sprouted with solutions containing different sucrose concentrations (10, 20, and 30 g/L).					
36108445	2	55	contain	containing	315:324	arg1	solutions					305:313	solutions	305:313	solutions containing different sucrose concentrations (10, 20, and 30 g/L)	305:378	Mung bean seeds were sprouted with solutions containing different sucrose concentrations (10, 20, and 30 g/L).					
36940200	6	0	theme	injured	1059:1065	arg1	limb					1067:1070	the injured limb	1055:1070	the injured limb	1055:1070	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	8	1	theme	kinematic	1333:1341	arg1	variability					1343:1353	kinematic variability	1333:1353	kinematic variability structure (KVstructure)	1333:1377	Frontal and sagittal plane kinematics were extracted, and sample entropy was used to calculate kinematic variability structure (KVstructure).					
36940200	7	2	theme	8-camera	1203:1210	arg1	system					1230:1235	an 8-camera 3D motion capture system	1200:1235	an 8-camera 3D motion capture system	1200:1235	Knee kinematics were collected at a self-selected comfortable walking speed on a treadmill with an 8-camera 3D motion capture system.					
36940200	1	3	theme	cartilage	283:291	arg1	composition					293:303	cartilage composition	283:303	cartilage composition linked to osteoarthritis development	283:340	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	8	4	theme	plane	1259:1263	arg1	kinematics					1265:1274	Frontal and sagittal plane kinematics	1238:1274	Frontal and sagittal plane kinematics	1238:1274	Frontal and sagittal plane kinematics were extracted, and sample entropy was used to calculate kinematic variability structure (KVstructure).					
36940200	0	5	attach	Linked	27:32	arg1	Reconstruction					74:87	Worse Cartilage Composition Post-ACL Reconstruction	37:87	Worse Cartilage Composition Post-ACL Reconstruction	37:87	Gait Variability Structure Linked to Worse Cartilage Composition Post-ACL Reconstruction.					
36940200	0	5	attach	Linked	27:32	arg2	Structure					17:25	Gait Variability Structure	0:25	Gait Variability Structure	0:25	Gait Variability Structure Linked to Worse Cartilage Composition Post-ACL Reconstruction.					
36940200	10	6	theme	mean	1567:1570	arg1	ILR					1576:1578	greater mean T1ρ ILR	1559:1578	greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03)	1559:1673	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	6	7	from	density	1014:1020	arg1	limb					1067:1070	the injured limb	1055:1070	the injured limb	1055:1070	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	0	8	theme	Gait	0:3	arg1	Structure					17:25	Gait Variability Structure	0:25	Gait Variability Structure	0:25	Gait Variability Structure Linked to Worse Cartilage Composition Post-ACL Reconstruction.					
36940200	10	9	theme	lateral	1596:1602	arg1	condyles					1645:1652	the anterior lateral (r = -0.44, p = .04) and anterior medial condyles	1583:1652	the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03)	1583:1673	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	11	10	theme	T1ρ	1739:1741	arg1	ILR					1743:1745	greater mean T1ρ ILR	1726:1745	greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03)	1726:1798	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	10	11	dep	lateral	1596:1602	arg1	=					1618:1618	=	1618:1618	=	1618:1618	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	3	12	theme	±	591:591	arg1	years					595:599	21 ± 4 years	588:599	13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR	576:633	METHODS T1ρ MRI and gait kinematics were collected in 22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR).					
36940200	11	13	theme	greater	1726:1732	arg1	ILR					1743:1745	greater mean T1ρ ILR	1726:1745	greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03)	1726:1798	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	13	14	theme	kinematic	2044:2052	arg1	variability					2054:2064	less knee kinematic variability	2034:2064	less knee kinematic variability	2034:2064	The findings suggest less knee kinematic variability structure is a mechanism linking aberrant gait to early osteoarthritis development.					
36940200	5	15	theme	Mean	828:831	arg1	times					848:852	Mean T1ρ relaxation times	828:852	Mean T1ρ relaxation times	828:852	Mean T1ρ relaxation times were extracted from each region and interlimb ratios (ILRs) were calculated (i.e., ACLR/uninjured limb).					
36940200	3	16	dep	participants	562:573	arg1	Females					579:585	13 Females	576:585	13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR	576:633	METHODS T1ρ MRI and gait kinematics were collected in 22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR).					
36940200	4	17	theme	Femoral	637:643	arg1	cartilage					655:663	Femoral articular cartilage	637:663	Femoral articular cartilage from the ACLR and uninjured limbs	637:697	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	11	18	theme	anterior	1754:1761	arg1	condyle					1771:1777	the anterior lateral condyle	1750:1777	the anterior lateral condyle (r = -0.47, p = .03)	1750:1798	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	1	19	from	changes	272:278	arg1	composition					293:303	cartilage composition	283:303	cartilage composition linked to osteoarthritis development	283:340	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	9	20	theme	T1ρ	1471:1473	arg1	variables					1491:1499	T1ρ and KVstructure variables	1471:1499	T1ρ and KVstructure variables	1471:1499	Pearson's product-moment correlations were conducted to determine the associations between T1ρ and KVstructure variables.					
36940200	12	21	theme	femoral	1864:1870	arg1	cartilage					1882:1890	worse femoral articular cartilage	1858:1890	worse femoral articular cartilage	1858:1890	CONCLUSIONS The association between less KVstructure and worse femoral articular cartilage proteoglycan density suggests a link between less variable knee kinematics and deleterious changes joint tissue changes.					
36940200	5	22	dep	limb	952:955	arg1	i.e.					931:934	i.e.	931:934	i.e.	931:934	Mean T1ρ relaxation times were extracted from each region and interlimb ratios (ILRs) were calculated (i.e., ACLR/uninjured limb).					
36940200	10	23	dep	KVstructure	1531:1541	arg1	associated					1543:1552	associated	1543:1552	KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03)	1531:1673	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	9	24	theme	KVstructure	1479:1489	arg1	variables					1491:1499	T1ρ and KVstructure variables	1471:1499	T1ρ and KVstructure variables	1471:1499	Pearson's product-moment correlations were conducted to determine the associations between T1ρ and KVstructure variables.					
36940200	4	25	theme	anterior	719:726	arg1	regions					752:758	anterior, central, and posterior regions	719:758	anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles	719:825	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	11	26	theme	Lesser	1676:1681	arg1	plane					1692:1696	Lesser sagittal plane	1676:1696	Lesser sagittal plane	1676:1696	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	3	27	theme	months	618:623	arg1	post-ACLR					625:633	7.52 ± 1.43 months post-ACLR	606:633	13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR	576:633	METHODS T1ρ MRI and gait kinematics were collected in 22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR).					
36940200	1	28	theme	anterior	157:164	arg1	ACLR					200:203	ACLR	200:203	ACLR	200:203	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	1	28	theme	anterior	157:164	arg1	reconstruction					184:197	anterior cruciate ligament reconstruction	157:197	anterior cruciate ligament reconstruction (ACLR)	157:204	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	12	29	theme	deleterious	1971:1981	arg1	changes					2004:2010	deleterious changes joint tissue changes	1971:2010	deleterious changes joint tissue changes	1971:2010	CONCLUSIONS The association between less KVstructure and worse femoral articular cartilage proteoglycan density suggests a link between less variable knee kinematics and deleterious changes joint tissue changes.					
36940200	0	30	theme	Post-ACL	65:72	arg1	Reconstruction					74:87	Worse Cartilage Composition Post-ACL Reconstruction	37:87	Worse Cartilage Composition Post-ACL Reconstruction	37:87	Gait Variability Structure Linked to Worse Cartilage Composition Post-ACL Reconstruction.					
36940200	3	31	theme	±	611:611	arg1	post-ACLR					625:633	7.52 ± 1.43 months post-ACLR	606:633	13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR	576:633	METHODS T1ρ MRI and gait kinematics were collected in 22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR).					
36940200	4	32	from	portions	783:790	arg1	regions					752:758	anterior, central, and posterior regions	719:758	anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles	719:825	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	1	33	theme	ligament	175:182	arg1	ACLR					200:203	ACLR	200:203	ACLR	200:203	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	1	33	theme	ligament	175:182	arg1	reconstruction					184:197	anterior cruciate ligament reconstruction	157:197	anterior cruciate ligament reconstruction (ACLR)	157:204	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	8	34	theme	sample	1296:1301	arg1	entropy					1303:1309	sample entropy	1296:1309	sample entropy	1296:1309	Frontal and sagittal plane kinematics were extracted, and sample entropy was used to calculate kinematic variability structure (KVstructure).					
36940200	5	35	theme	interlimb	890:898	arg1	ILRs					908:911	ILRs	908:911	ILRs	908:911	Mean T1ρ relaxation times were extracted from each region and interlimb ratios (ILRs) were calculated (i.e., ACLR/uninjured limb).					
36940200	5	35	theme	interlimb	890:898	arg1	ratios					900:905	interlimb ratios	890:905	interlimb ratios (ILRs)	890:912	Mean T1ρ relaxation times were extracted from each region and interlimb ratios (ILRs) were calculated (i.e., ACLR/uninjured limb).					
36940200	6	36	theme	T1ρ	967:969	arg1	ILRs					971:974	Greater T1ρ ILRs	959:974	Greater T1ρ ILRs	959:974	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	6	36	theme	T1ρ	967:969	arg1	density					1014:1020	less proteoglycan density	996:1020	less proteoglycan density (worse cartilage composition) in the injured limb	996:1070	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	13	37	theme	aberrant	2099:2106	arg1	gait					2108:2111	aberrant gait	2099:2111	aberrant gait	2099:2111	The findings suggest less knee kinematic variability structure is a mechanism linking aberrant gait to early osteoarthritis development.					
36940200	10	38	dep	=	1657:1657	arg1	-0.47					1659:1663	-0.47	1659:1663	-0.47	1659:1663	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	5	39	theme	ACLR/uninjured	937:950	arg1	limb					952:955	ACLR/uninjured limb	937:955	ACLR/uninjured limb	937:955	Mean T1ρ relaxation times were extracted from each region and interlimb ratios (ILRs) were calculated (i.e., ACLR/uninjured limb).					
36940200	2	40	theme	resonance	437:445	arg1	times					472:476	T1ρ magnetic resonance imaging (MRI) relaxation times	424:476	T1ρ magnetic resonance imaging (MRI) relaxation times	424:476	Our purpose was to determine the association between femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability.					
36940200	7	41	theme	walking	1166:1172	arg1	speed					1174:1178	a self-selected comfortable walking speed	1138:1178	a self-selected comfortable walking speed	1138:1178	Knee kinematics were collected at a self-selected comfortable walking speed on a treadmill with an 8-camera 3D motion capture system.					
36940200	1	42	theme	INTRODUCTION	90:101	arg1	variability					117:127	INTRODUCTION Aberrant gait variability	90:127	INTRODUCTION Aberrant gait variability	90:127	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	2	43	theme	gait	482:485	arg1	variability					487:497	gait variability	482:497	gait variability	482:497	Our purpose was to determine the association between femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability.					
36940200	4	44	theme	central	729:735	arg1	regions					752:758	anterior, central, and posterior regions	719:758	anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles	719:825	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	2	45	theme	T1ρ	424:426	arg1	times					472:476	T1ρ magnetic resonance imaging (MRI) relaxation times	424:476	T1ρ magnetic resonance imaging (MRI) relaxation times	424:476	Our purpose was to determine the association between femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability.					
36940200	7	46	theme	self-selected	1140:1152	arg1	speed					1174:1178	a self-selected comfortable walking speed	1138:1178	a self-selected comfortable walking speed	1138:1178	Knee kinematics were collected at a self-selected comfortable walking speed on a treadmill with an 8-camera 3D motion capture system.					
36940200	1	47	theme	gait	112:115	arg1	variability					117:127	INTRODUCTION Aberrant gait variability	90:127	INTRODUCTION Aberrant gait variability	90:127	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	6	48	theme	cartilage	1029:1037	arg1	composition					1039:1049	worse cartilage composition	1023:1049	worse cartilage composition	1023:1049	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	6	48	theme	cartilage	1029:1037	arg1	density					1014:1020	less proteoglycan density	996:1020	less proteoglycan density (worse cartilage composition) in the injured limb	996:1070	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	11	49	dep	=	1793:1793	arg1	r					1780:1780	r	1780:1780	r = -0.47	1780:1788	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	1	50	theme	cruciate	166:173	arg1	ACLR					200:203	ACLR	200:203	ACLR	200:203	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	1	50	theme	cruciate	166:173	arg1	reconstruction					184:197	anterior cruciate ligament reconstruction	157:197	anterior cruciate ligament reconstruction (ACLR)	157:204	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	6	51	theme	proteoglycan	1001:1012	arg1	composition					1039:1049	worse cartilage composition	1023:1049	worse cartilage composition	1023:1049	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	6	51	theme	proteoglycan	1001:1012	arg1	ILRs					971:974	Greater T1ρ ILRs	959:974	Greater T1ρ ILRs	959:974	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	6	51	theme	proteoglycan	1001:1012	arg1	density					1014:1020	less proteoglycan density	996:1020	less proteoglycan density (worse cartilage composition) in the injured limb	996:1070	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	10	52	dep	RESULTS	1502:1508	arg1	plane					1525:1529	Lesser frontal plane	1510:1529	RESULTS Lesser frontal plane	1502:1529	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	4	53	theme	condyles	818:825	arg1	portions					783:790	the weightbearing portions	765:790	the weightbearing portions of the medial and lateral condyles	765:825	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	3	54	theme	gait	520:523	arg1	kinematics					525:534	gait kinematics	520:534	gait kinematics	520:534	METHODS T1ρ MRI and gait kinematics were collected in 22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR).					
36940200	0	55	theme	Cartilage	43:51	arg1	Reconstruction					74:87	Worse Cartilage Composition Post-ACL Reconstruction	37:87	Worse Cartilage Composition Post-ACL Reconstruction	37:87	Gait Variability Structure Linked to Worse Cartilage Composition Post-ACL Reconstruction.					
36940200	6	56	theme	uninjured	1088:1096	arg1	limb					1098:1101	the uninjured limb	1084:1101	the uninjured limb	1084:1101	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	10	57	theme	Lesser	1510:1515	arg1	plane					1525:1529	Lesser frontal plane	1510:1529	RESULTS Lesser frontal plane	1502:1529	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	10	58	dep	=	1618:1618	arg1	r					1605:1605	r	1605:1605	r = -0.44	1605:1613	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	1	59	theme	gait	233:236	arg1	variability					238:248	gait variability	233:248	gait variability	233:248	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	10	60	theme	greater	1559:1565	arg1	ILR					1576:1578	greater mean T1ρ ILR	1559:1578	greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03)	1559:1673	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	2	61	theme	articular	404:412	arg1	cartilage					414:422	femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability	396:497	cartilage	414:422	Our purpose was to determine the association between femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability.					
36940200	13	62	theme	early	2116:2120	arg1	development					2137:2147	early osteoarthritis development	2116:2147	early osteoarthritis development	2116:2147	The findings suggest less knee kinematic variability structure is a mechanism linking aberrant gait to early osteoarthritis development.					
36940200	12	63	theme	changes	1983:1989	arg1	changes					2004:2010	deleterious changes joint tissue changes	1971:2010	deleterious changes joint tissue changes	1971:2010	CONCLUSIONS The association between less KVstructure and worse femoral articular cartilage proteoglycan density suggests a link between less variable knee kinematics and deleterious changes joint tissue changes.					
36940200	7	64	theme	capture	1222:1228	arg1	system					1230:1235	an 8-camera 3D motion capture system	1200:1235	an 8-camera 3D motion capture system	1200:1235	Knee kinematics were collected at a self-selected comfortable walking speed on a treadmill with an 8-camera 3D motion capture system.					
36940200	10	65	dep	=	1607:1607	arg1	-0.44					1609:1613	-0.44	1609:1613	-0.44	1609:1613	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	1	66	theme	early	266:270	arg1	changes					272:278	early changes	266:278	early changes in cartilage composition linked to osteoarthritis development	266:340	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	12	67	theme	knee	1951:1954	arg1	kinematics					1956:1965	less variable knee kinematics	1937:1965	less variable knee kinematics	1937:1965	CONCLUSIONS The association between less KVstructure and worse femoral articular cartilage proteoglycan density suggests a link between less variable knee kinematics and deleterious changes joint tissue changes.					
36940200	7	68	theme	3D	1212:1213	arg1	system					1230:1235	an 8-camera 3D motion capture system	1200:1235	an 8-camera 3D motion capture system	1200:1235	Knee kinematics were collected at a self-selected comfortable walking speed on a treadmill with an 8-camera 3D motion capture system.					
36940200	10	69	dep	condyles	1645:1652	arg1	=					1668:1668	=	1668:1668	=	1668:1668	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	1	70	attach	linked	305:310	arg2	composition					293:303	cartilage composition	283:303	cartilage composition linked to osteoarthritis development	283:340	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	1	70	attach	linked	305:310	arg1	development					330:340	osteoarthritis development	315:340	osteoarthritis development	315:340	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	9	71	theme	product-moment	1390:1403	arg1	correlations					1405:1416	Pearson's product-moment correlations	1380:1416	Pearson's product-moment correlations	1380:1416	Pearson's product-moment correlations were conducted to determine the associations between T1ρ and KVstructure variables.					
36940200	12	72	theme	joint	1991:1995	arg1	changes					2004:2010	deleterious changes joint tissue changes	1971:2010	deleterious changes joint tissue changes	1971:2010	CONCLUSIONS The association between less KVstructure and worse femoral articular cartilage proteoglycan density suggests a link between less variable knee kinematics and deleterious changes joint tissue changes.					
36940200	13	73	dep	suggest	2026:2032	arg1	structure					2066:2074	structure	2066:2074	structure	2066:2074	The findings suggest less knee kinematic variability structure is a mechanism linking aberrant gait to early osteoarthritis development.					
36940200	13	73	dep	suggest	2026:2032	arg1	mechanism					2081:2089	a mechanism	2079:2089	a mechanism linking aberrant gait to early osteoarthritis development	2079:2147	The findings suggest less knee kinematic variability structure is a mechanism linking aberrant gait to early osteoarthritis development.					
36940200	10	74	theme	T1ρ	1572:1574	arg1	ILR					1576:1578	greater mean T1ρ ILR	1559:1578	greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03)	1559:1673	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	3	75	theme	ACLR	557:560	arg1	participants					562:573	22 ACLR participants	554:573	22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR)	554:634	METHODS T1ρ MRI and gait kinematics were collected in 22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR).					
36940200	7	76	theme	Knee	1104:1107	arg1	kinematics					1109:1118	Knee kinematics	1104:1118	Knee kinematics	1104:1118	Knee kinematics were collected at a self-selected comfortable walking speed on a treadmill with an 8-camera 3D motion capture system.					
36940200	4	77	theme	articular	645:653	arg1	cartilage					655:663	Femoral articular cartilage	637:663	Femoral articular cartilage from the ACLR and uninjured limbs	637:697	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	12	78	dep	CONCLUSIONS	1801:1811	arg1	suggests					1913:1920	suggests	1913:1920	suggests a link between less variable knee kinematics and deleterious changes joint tissue changes	1913:2010	CONCLUSIONS The association between less KVstructure and worse femoral articular cartilage proteoglycan density suggests a link between less variable knee kinematics and deleterious changes joint tissue changes.					
36940200	12	79	theme	articular	1872:1880	arg1	cartilage					1882:1890	worse femoral articular cartilage	1858:1890	worse femoral articular cartilage	1858:1890	CONCLUSIONS The association between less KVstructure and worse femoral articular cartilage proteoglycan density suggests a link between less variable knee kinematics and deleterious changes joint tissue changes.					
36940200	11	80	from	ILR	1743:1745	arg1	condyle					1771:1777	the anterior lateral condyle	1750:1777	the anterior lateral condyle (r = -0.47, p = .03)	1750:1798	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	10	81	theme	anterior	1587:1594	arg1	condyles					1645:1652	the anterior lateral (r = -0.44, p = .04) and anterior medial condyles	1583:1652	the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03)	1583:1673	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	12	82	theme	proteoglycan	1892:1903	arg1	density					1905:1911	proteoglycan density	1892:1911	proteoglycan density	1892:1911	CONCLUSIONS The association between less KVstructure and worse femoral articular cartilage proteoglycan density suggests a link between less variable knee kinematics and deleterious changes joint tissue changes.					
36940200	4	83	theme	uninjured	683:691	arg1	limbs					693:697	the ACLR and uninjured limbs	670:697	the ACLR and uninjured limbs	670:697	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	11	84	theme	mean	1734:1737	arg1	ILR					1743:1745	greater mean T1ρ ILR	1726:1745	greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03)	1726:1798	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	3	85	theme	old	601:603	arg1	years					595:599	21 ± 4 years	588:599	13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR	576:633	METHODS T1ρ MRI and gait kinematics were collected in 22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR).					
36940200	5	86	theme	T1ρ	833:835	arg1	times					848:852	Mean T1ρ relaxation times	828:852	Mean T1ρ relaxation times	828:852	Mean T1ρ relaxation times were extracted from each region and interlimb ratios (ILRs) were calculated (i.e., ACLR/uninjured limb).					
36940200	8	87	theme	Frontal	1238:1244	arg1	kinematics					1265:1274	Frontal and sagittal plane kinematics	1238:1274	Frontal and sagittal plane kinematics	1238:1274	Frontal and sagittal plane kinematics were extracted, and sample entropy was used to calculate kinematic variability structure (KVstructure).					
36940200	10	88	theme	=	1607:1607	arg1	r					1605:1605	r	1605:1605	r = -0.44	1605:1613	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	11	89	theme	lateral	1763:1769	arg1	condyle					1771:1777	the anterior lateral condyle	1750:1777	the anterior lateral condyle (r = -0.47, p = .03)	1750:1798	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	12	90	theme	worse	1858:1862	arg1	cartilage					1882:1890	worse femoral articular cartilage	1858:1890	worse femoral articular cartilage	1858:1890	CONCLUSIONS The association between less KVstructure and worse femoral articular cartilage proteoglycan density suggests a link between less variable knee kinematics and deleterious changes joint tissue changes.					
36940200	12	91	theme	tissue	1997:2002	arg1	changes					2004:2010	deleterious changes joint tissue changes	1971:2010	deleterious changes joint tissue changes	1971:2010	CONCLUSIONS The association between less KVstructure and worse femoral articular cartilage proteoglycan density suggests a link between less variable knee kinematics and deleterious changes joint tissue changes.					
36940200	8	92	theme	sagittal	1250:1257	arg1	kinematics					1265:1274	Frontal and sagittal plane kinematics	1238:1274	Frontal and sagittal plane kinematics	1238:1274	Frontal and sagittal plane kinematics were extracted, and sample entropy was used to calculate kinematic variability structure (KVstructure).					
36940200	11	93	dep	KVstructure	1698:1708	arg1	associated					1710:1719	associated	1710:1719	KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03)	1698:1798	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	7	94	with	treadmill	1185:1193	arg1	system					1230:1235	an 8-camera 3D motion capture system	1200:1235	an 8-camera 3D motion capture system	1200:1235	Knee kinematics were collected at a self-selected comfortable walking speed on a treadmill with an 8-camera 3D motion capture system.					
36940200	11	95	theme	sagittal	1683:1690	arg1	plane					1692:1696	Lesser sagittal plane	1676:1696	Lesser sagittal plane	1676:1696	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	13	96	theme	knee	2039:2042	arg1	variability					2054:2064	less knee kinematic variability	2034:2064	less knee kinematic variability	2034:2064	The findings suggest less knee kinematic variability structure is a mechanism linking aberrant gait to early osteoarthritis development.					
36940200	3	97	theme	7.52	606:609	arg1	post-ACLR					625:633	7.52 ± 1.43 months post-ACLR	606:633	13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR	576:633	METHODS T1ρ MRI and gait kinematics were collected in 22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR).					
36940200	3	98	dep	Females	579:585	arg1	post-ACLR					625:633	7.52 ± 1.43 months post-ACLR	606:633	13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR	576:633	METHODS T1ρ MRI and gait kinematics were collected in 22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR).					
36940200	3	98	dep	Females	579:585	arg1	years					595:599	21 ± 4 years	588:599	13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR	576:633	METHODS T1ρ MRI and gait kinematics were collected in 22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR).					
36940200	4	99	theme	ACLR	674:677	arg1	limbs					693:697	the ACLR and uninjured limbs	670:697	the ACLR and uninjured limbs	670:697	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	5	100	dep	calculated	919:928	arg1	limb					952:955	ACLR/uninjured limb	937:955	ACLR/uninjured limb	937:955	Mean T1ρ relaxation times were extracted from each region and interlimb ratios (ILRs) were calculated (i.e., ACLR/uninjured limb).					
36940200	2	101	dep	cartilage	414:422	arg1	times					472:476	T1ρ magnetic resonance imaging (MRI) relaxation times	424:476	T1ρ magnetic resonance imaging (MRI) relaxation times	424:476	Our purpose was to determine the association between femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability.					
36940200	11	102	dep	condyle	1771:1777	arg1	=					1793:1793	=	1793:1793	=	1793:1793	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	6	103	theme	Greater	959:965	arg1	ILRs					971:974	Greater T1ρ ILRs	959:974	Greater T1ρ ILRs	959:974	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	6	103	theme	Greater	959:965	arg1	density					1014:1020	less proteoglycan density	996:1020	less proteoglycan density (worse cartilage composition) in the injured limb	996:1070	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	12	104	dep	KVstructure	1842:1852	arg1	density					1905:1911	proteoglycan density	1892:1911	proteoglycan density	1892:1911	CONCLUSIONS The association between less KVstructure and worse femoral articular cartilage proteoglycan density suggests a link between less variable knee kinematics and deleterious changes joint tissue changes.					
36940200	4	105	theme	posterior	742:750	arg1	regions					752:758	anterior, central, and posterior regions	719:758	anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles	719:825	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	7	106	theme	comfortable	1154:1164	arg1	speed					1174:1178	a self-selected comfortable walking speed	1138:1178	a self-selected comfortable walking speed	1138:1178	Knee kinematics were collected at a self-selected comfortable walking speed on a treadmill with an 8-camera 3D motion capture system.					
36940200	10	107	dep	=	1668:1668	arg1	r					1655:1655	r	1655:1655	r = -0.47	1655:1663	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	2	108	theme	magnetic	428:435	arg1	resonance					437:445	magnetic resonance imaging	428:453	T1ρ magnetic resonance imaging (MRI) relaxation times	424:476	Our purpose was to determine the association between femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability.					
36940200	2	108	theme	magnetic	428:435	arg1	MRI					456:458	MRI	456:458	MRI	456:458	Our purpose was to determine the association between femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability.					
36940200	0	109	theme	Variability	5:15	arg1	Structure					17:25	Gait Variability Structure	0:25	Gait Variability Structure	0:25	Gait Variability Structure Linked to Worse Cartilage Composition Post-ACL Reconstruction.					
36940200	1	110	theme	Aberrant	103:110	arg1	variability					117:127	INTRODUCTION Aberrant gait variability	90:127	INTRODUCTION Aberrant gait variability	90:127	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	10	111	from	ILR	1576:1578	arg1	condyles					1645:1652	the anterior lateral (r = -0.44, p = .04) and anterior medial condyles	1583:1652	the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03)	1583:1673	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	6	112	theme	worse	1023:1027	arg1	composition					1039:1049	worse cartilage composition	1023:1049	worse cartilage composition	1023:1049	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	6	112	theme	worse	1023:1027	arg1	density					1014:1020	less proteoglycan density	996:1020	less proteoglycan density (worse cartilage composition) in the injured limb	996:1070	Greater T1ρ ILRs were interpreted as less proteoglycan density (worse cartilage composition) in the injured limb compared to the uninjured limb.					
36940200	2	113	theme	relaxation	461:470	arg1	times					472:476	T1ρ magnetic resonance imaging (MRI) relaxation times	424:476	T1ρ magnetic resonance imaging (MRI) relaxation times	424:476	Our purpose was to determine the association between femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability.					
36940200	8	114	theme	variability	1343:1353	arg1	KVstructure					1366:1376	KVstructure	1366:1376	KVstructure	1366:1376	Frontal and sagittal plane kinematics were extracted, and sample entropy was used to calculate kinematic variability structure (KVstructure).					
36940200	8	114	theme	variability	1343:1353	arg1	structure					1355:1363	kinematic variability structure	1333:1363	kinematic variability structure (KVstructure)	1333:1377	Frontal and sagittal plane kinematics were extracted, and sample entropy was used to calculate kinematic variability structure (KVstructure).					
36940200	10	115	theme	medial	1638:1643	arg1	condyles					1645:1652	the anterior lateral (r = -0.44, p = .04) and anterior medial condyles	1583:1652	the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03)	1583:1673	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	0	116	theme	Worse	37:41	arg1	Reconstruction					74:87	Worse Cartilage Composition Post-ACL Reconstruction	37:87	Worse Cartilage Composition Post-ACL Reconstruction	37:87	Gait Variability Structure Linked to Worse Cartilage Composition Post-ACL Reconstruction.					
36940200	1	117	theme	osteoarthritis	315:328	arg1	development					330:340	osteoarthritis development	315:340	osteoarthritis development	315:340	INTRODUCTION Aberrant gait variability has been observed following anterior cruciate ligament reconstruction (ACLR), yet it remains unknown if gait variability associates with early changes in cartilage composition linked to osteoarthritis development.					
36940200	8	118	used	used	1315:1318	arg2	entropy					1303:1309	sample entropy	1296:1309	sample entropy	1296:1309	Frontal and sagittal plane kinematics were extracted, and sample entropy was used to calculate kinematic variability structure (KVstructure).					
36940200	4	119	theme	medial	799:804	arg1	condyles					818:825	the medial and lateral condyles	795:825	the medial and lateral condyles	795:825	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	2	120	theme	imaging	447:453	arg1	resonance					437:445	magnetic resonance imaging	428:453	T1ρ magnetic resonance imaging (MRI) relaxation times	424:476	Our purpose was to determine the association between femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability.					
36940200	2	120	theme	imaging	447:453	arg1	MRI					456:458	MRI	456:458	MRI	456:458	Our purpose was to determine the association between femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability.					
36940200	0	121	theme	Composition	53:63	arg1	Reconstruction					74:87	Worse Cartilage Composition Post-ACL Reconstruction	37:87	Worse Cartilage Composition Post-ACL Reconstruction	37:87	Gait Variability Structure Linked to Worse Cartilage Composition Post-ACL Reconstruction.					
36940200	10	122	theme	frontal	1517:1523	arg1	plane					1525:1529	Lesser frontal plane	1510:1529	RESULTS Lesser frontal plane	1502:1529	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	4	123	theme	lateral	810:816	arg1	condyles					818:825	the medial and lateral condyles	795:825	the medial and lateral condyles	795:825	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	10	124	theme	=	1657:1657	arg1	r					1655:1655	r	1655:1655	r = -0.47	1655:1663	RESULTS Lesser frontal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral (r = -0.44, p = .04) and anterior medial condyles (r = -0.47, p = .03).					
36940200	11	125	theme	=	1782:1782	arg1	r					1780:1780	r	1780:1780	r = -0.47	1780:1788	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	11	126	dep	=	1782:1782	arg1	-0.47					1784:1788	-0.47	1784:1788	-0.47	1784:1788	Lesser sagittal plane KVstructure associated with greater mean T1ρ ILR in the anterior lateral condyle (r = -0.47, p = .03).					
36940200	4	127	theme	weightbearing	769:781	arg1	portions					783:790	the weightbearing portions	765:790	the weightbearing portions of the medial and lateral condyles	765:825	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	12	128	theme	variable	1942:1949	arg1	kinematics					1956:1965	less variable knee kinematics	1937:1965	less variable knee kinematics	1937:1965	CONCLUSIONS The association between less KVstructure and worse femoral articular cartilage proteoglycan density suggests a link between less variable knee kinematics and deleterious changes joint tissue changes.					
36940200	5	129	theme	relaxation	837:846	arg1	times					848:852	Mean T1ρ relaxation times	828:852	Mean T1ρ relaxation times	828:852	Mean T1ρ relaxation times were extracted from each region and interlimb ratios (ILRs) were calculated (i.e., ACLR/uninjured limb).					
36940200	3	130	dep	METHODS	500:506	arg1	collected					541:549	collected	541:549	were collected in 22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR)	536:634	METHODS T1ρ MRI and gait kinematics were collected in 22 ACLR participants (13 Females; 21 ± 4 years old; 7.52 ± 1.43 months post-ACLR).					
36940200	7	131	theme	motion	1215:1220	arg1	system					1230:1235	an 8-camera 3D motion capture system	1200:1235	an 8-camera 3D motion capture system	1200:1235	Knee kinematics were collected at a self-selected comfortable walking speed on a treadmill with an 8-camera 3D motion capture system.					
36940200	2	132	theme	femoral	396:402	arg1	cartilage					414:422	femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability	396:497	cartilage	414:422	Our purpose was to determine the association between femoral articular cartilage T1ρ magnetic resonance imaging (MRI) relaxation times and gait variability.					
36940200	4	133	from	limbs	693:697	arg1	cartilage					655:663	Femoral articular cartilage	637:663	Femoral articular cartilage from the ACLR and uninjured limbs	637:697	Femoral articular cartilage from the ACLR and uninjured limbs were segmented into anterior, central, and posterior regions from the weightbearing portions of the medial and lateral condyles.					
36940200	13	134	theme	osteoarthritis	2122:2135	arg1	development					2137:2147	early osteoarthritis development	2116:2147	early osteoarthritis development	2116:2147	The findings suggest less knee kinematic variability structure is a mechanism linking aberrant gait to early osteoarthritis development.					
35468155	9	0	theme	crystalline	1513:1523	arg1	nature					1525:1530	the crystalline nature	1509:1530	the crystalline nature of the pure drug	1509:1547	FTIR analysis showed that the composition of the particles was completely compatible, while XRD analysis confirmed the crystalline nature of the pure drug and its transition into an amorphous state after formulation.					
35468155	4	1	theme	in	945:946	arg1	percentage					954:963	in vitro percentage	945:963	in vitro percentage of cumulative drug release (R4)	945:995	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	6	2	theme	oral	1150:1153	arg1	determination					1164:1176	in-vivo acute oral toxicity determination	1136:1176	in-vivo acute oral toxicity determination	1136:1176	Biocompatibility and non-toxicity were confirmed by in-vivo acute oral toxicity determination.					
35468155	10	3	theme	DSC	1611:1613	arg1	analysis					1615:1622	DSC analysis	1611:1622	DSC analysis	1611:1622	DSC analysis revealed the thermal stability of the formulations.					
35468155	1	4	theme	colon	280:284	arg1	targeting					286:294	colon targeting	280:294	colon targeting	280:294	The study aimed to fabricate and evaluate Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting because MLX is a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC).					
35468155	6	5	theme	in-vivo	1136:1142	arg1	determination					1164:1176	in-vivo acute oral toxicity determination	1136:1176	in-vivo acute oral toxicity determination	1136:1176	Biocompatibility and non-toxicity were confirmed by in-vivo acute oral toxicity determination.					
35468155	9	6	theme	pure	1539:1542	arg1	drug					1544:1547	the pure drug	1535:1547	the pure drug	1535:1547	FTIR analysis showed that the composition of the particles was completely compatible, while XRD analysis confirmed the crystalline nature of the pure drug and its transition into an amorphous state after formulation.					
35468155	10	7	theme	thermal	1637:1643	arg1	stability					1645:1653	the thermal stability	1633:1653	the thermal stability of the formulations	1633:1673	DSC analysis revealed the thermal stability of the formulations.					
35468155	1	8	theme	Meloxicam	201:209	arg1	microparticles					261:274	Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles	201:274	Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting	201:294	The study aimed to fabricate and evaluate Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting because MLX is a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC).					
35468155	12	9	from	study	1740:1744	arg1	rabbits					1756:1762	albino rabbits	1749:1762	albino rabbits	1749:1762	The toxicity study in albino rabbits showed no toxicity and was found biocompatible.					
35468155	4	10	theme	release	984:990	arg1	R1					875:876	R1	875:876	R1	875:876	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	10	theme	release	984:990	arg1	EE					907:908	EE	907:908	EE	907:908	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	10	theme	release	984:990	arg1	size					931:934	mean particle size	917:934	mean particle size (R3)	917:939	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	10	theme	release	984:990	arg1	percentage					954:963	in vitro percentage	945:963	in vitro percentage of cumulative drug release (R4)	945:995	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	10	theme	release	984:990	arg1	R3					937:938	R3	937:938	R3	937:938	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	10	theme	release	984:990	arg1	R2					912:913	R2	912:913	R2	912:913	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	10	theme	release	984:990	arg1	efficiency					895:904	the entrapment efficiency	880:904	the entrapment efficiency (EE) (R2)	880:914	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	10	theme	release	984:990	arg1	yield					868:872	the percent yield	856:872	the percent yield (R1)	856:877	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	0	11	theme	in-vivo	135:141	arg1	study					152:156	in-vivo toxicity study	135:156	in-vivo toxicity study	135:156	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	0	11	theme	in-vivo	135:141	arg1	microparticles					27:40	microparticles	27:40	microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study	27:156	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	14	12	theme	side	2033:2036	arg1	effects					2038:2044	GIT drug-associated side effects	2013:2044	GIT drug-associated side effects in CRC treatment	2013:2061	Results of this study highlighted a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment.					
35468155	10	13	theme	formulations	1662:1673	arg1	stability					1645:1653	the thermal stability	1633:1653	the thermal stability of the formulations	1633:1673	DSC analysis revealed the thermal stability of the formulations.					
35468155	1	14	theme	loaded	217:222	arg1	microparticles					261:274	Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles	201:274	Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting	201:294	The study aimed to fabricate and evaluate Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting because MLX is a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC).					
35468155	4	15	theme	3-level	692:698	arg1	design					730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design	680:735	design	730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	14	16	theme	GIT	2013:2015	arg1	effects					2038:2044	GIT drug-associated side effects	2013:2044	GIT drug-associated side effects in CRC treatment	2013:2061	Results of this study highlighted a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment.					
35468155	4	17	theme	percent	860:866	arg1	R1					875:876	R1	875:876	R1	875:876	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	17	theme	percent	860:866	arg1	yield					868:872	the percent yield	856:872	the percent yield (R1)	856:877	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	18	theme	Behnken	704:710	arg1	design					730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design	680:735	design	730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	3	19	theme	O/O	628:630	arg1	technique					669:677	the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique	612:677	the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique	612:677	Seventeen formulations of MLX loaded HPMC microparticles were fabricated by the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique.					
35468155	4	20	from	effects	771:777	arg1	variables					825:833	the dependent variables	811:833	the dependent variables (responses)	811:845	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	20	from	effects	771:777	arg1	size					931:934	mean particle size	917:934	mean particle size (R3)	917:939	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	20	from	effects	771:777	arg1	percentage					954:963	in vitro percentage	945:963	in vitro percentage of cumulative drug release (R4)	945:995	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	20	from	effects	771:777	arg1	responses					836:844	responses	836:844	responses	836:844	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	20	from	effects	771:777	arg1	efficiency					895:904	the entrapment efficiency	880:904	the entrapment efficiency (EE) (R2)	880:914	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	20	from	effects	771:777	arg1	yield					868:872	the percent yield	856:872	the percent yield (R1)	856:877	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	8	21	theme	drug	1339:1342	arg1	percentage					1352:1361	the in vitro cumulative drug release percentage	1315:1361	the in vitro cumulative drug release percentage	1315:1361	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	8	21	theme	drug	1339:1342	arg1	%					1378:1378	74.25-92.64%	1367:1378	74.25-92.64%	1367:1378	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	3	22	theme	oil-in-oil	616:625	arg1	technique					669:677	the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique	612:677	the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique	612:677	Seventeen formulations of MLX loaded HPMC microparticles were fabricated by the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique.					
35468155	2	23	theme	gastrointestinal	481:496	arg1	tracts					498:503	gastrointestinal tracts	481:503	gastrointestinal tracts (GIT)	481:509	Nevertheless, its efficiency is limited by poor solubility and gastrointestinal tracts (GIT) associated side effects.					
35468155	2	23	theme	gastrointestinal	481:496	arg1	GIT					506:508	GIT	506:508	GIT	506:508	Nevertheless, its efficiency is limited by poor solubility and gastrointestinal tracts (GIT) associated side effects.					
35468155	4	24	theme	3-factor	682:689	arg1	design					730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design	680:735	design	730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	5	25	theme	physicochemical	1002:1016	arg1	characterization					1018:1033	physicochemical characterization	1002:1033	physicochemical characterization	1002:1033	For physicochemical characterization FTIR, XRD, DSC, and SEM analyses were performed.					
35468155	2	26	theme	side	522:525	arg1	effects					527:533	associated side effects	511:533	associated side effects	511:533	Nevertheless, its efficiency is limited by poor solubility and gastrointestinal tracts (GIT) associated side effects.					
35468155	0	27	theme	physicochemical	97:111	arg1	characterization					113:128	physicochemical characterization	97:128	physicochemical characterization	97:128	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	0	27	theme	physicochemical	97:111	arg1	microparticles					27:40	microparticles	27:40	microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study	27:156	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	1	28	theme	pain	355:358	arg1	treatment					342:350	the treatment	338:350	the treatment of pain and inflammation associated with colorectal cancer (CRC)	338:415	The study aimed to fabricate and evaluate Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting because MLX is a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC).					
35468155	1	29	theme	inflammation	364:375	arg1	treatment					342:350	the treatment	338:350	the treatment of pain and inflammation associated with colorectal cancer (CRC)	338:415	The study aimed to fabricate and evaluate Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting because MLX is a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC).					
35468155	12	30	theme	albino	1749:1754	arg1	rabbits					1756:1762	albino rabbits	1749:1762	albino rabbits	1749:1762	The toxicity study in albino rabbits showed no toxicity and was found biocompatible.					
35468155	4	31	theme	BBD	713:715	arg1	design					730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design	680:735	design	730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	0	32	theme	Developed	0:8	arg1	meloxicam					10:18	Developed meloxicam	0:18	Developed meloxicam	0:18	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	4	33	theme	statistical	718:728	arg1	design					730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design	680:735	design	730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	14	34	theme	delivery	1940:1947	arg1	system					1949:1954	a standard colonic drug delivery system	1916:1954	a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment	1916:2061	Results of this study highlighted a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment.					
35468155	3	35	theme	solvent	643:649	arg1	ESE					664:666	ESE	664:666	ESE	664:666	Seventeen formulations of MLX loaded HPMC microparticles were fabricated by the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique.					
35468155	3	35	theme	solvent	643:649	arg1	evaporation					651:661	emulsion solvent evaporation	634:661	the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique	612:677	Seventeen formulations of MLX loaded HPMC microparticles were fabricated by the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique.					
35468155	8	36	dep	in	1319:1320	arg1	vitro					1322:1326	vitro	1322:1326	vitro	1322:1326	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	7	37	theme	percentage	1183:1192	arg1	yield					1194:1198	The percentage yield	1179:1198	The percentage yield	1179:1198	The percentage yield and EE were 65.75-90.71%, and 70.62-88.37%, respectively.					
35468155	7	37	theme	percentage	1183:1192	arg1	%					1223:1223	65.75-90.71%	1212:1223	65.75-90.71%	1212:1223	The percentage yield and EE were 65.75-90.71%, and 70.62-88.37%, respectively.					
35468155	4	38	theme	mean	917:920	arg1	size					931:934	mean particle size	917:934	mean particle size (R3)	917:939	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	38	theme	mean	917:920	arg1	R3					937:938	R3	937:938	R3	937:938	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	14	39	theme	colonic	1927:1933	arg1	system					1949:1954	a standard colonic drug delivery system	1916:1954	a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment	1916:2061	Results of this study highlighted a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment.					
35468155	4	40	theme	variables	798:806	arg1	effects					771:777	the combined effects	758:777	the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4)	758:995	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	0	41	theme	targeted	52:59	arg1	delivery					61:68	colon targeted delivery	46:68	colon targeted delivery	46:68	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	11	42	theme	dense	1700:1704	arg1	particles					1716:1724	dense spherical particles	1700:1724	dense spherical particles	1700:1724	The SEM analysis showed dense spherical particles.					
35468155	1	43	theme	Hydroxypropyl	224:236	arg1	microparticles					261:274	Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles	201:274	Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting	201:294	The study aimed to fabricate and evaluate Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting because MLX is a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC).					
35468155	4	44	theme	drug	979:982	arg1	R4					993:994	R4	993:994	R4	993:994	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	44	theme	drug	979:982	arg1	release					984:990	cumulative drug release	968:990	cumulative drug release (R4)	968:995	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	13	45	theme	altered	1864:1870	arg1	patterns					1872:1879	altered patterns	1864:1879	altered patterns	1864:1879	The histopathological evaluation showed no signs of altered patterns.					
35468155	11	46	theme	SEM	1680:1682	arg1	analysis					1684:1691	The SEM analysis	1676:1691	The SEM analysis	1676:1691	The SEM analysis showed dense spherical particles.					
35468155	6	47	theme	toxicity	1155:1162	arg1	determination					1164:1176	in-vivo acute oral toxicity determination	1136:1176	in-vivo acute oral toxicity determination	1136:1176	Biocompatibility and non-toxicity were confirmed by in-vivo acute oral toxicity determination.					
35468155	8	48	theme	particle	1276:1283	arg1	size					1285:1288	the mean particle size	1267:1288	the mean particle size	1267:1288	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	8	48	theme	particle	1276:1283	arg1	μm					1307:1308	62.89-284.55 μm	1294:1308	62.89-284.55 μm	1294:1308	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	6	49	theme	acute	1144:1148	arg1	determination					1164:1176	in-vivo acute oral toxicity determination	1136:1176	in-vivo acute oral toxicity determination	1136:1176	Biocompatibility and non-toxicity were confirmed by in-vivo acute oral toxicity determination.					
35468155	14	50	with	system	1949:1954	arg1	ability					1965:1971	the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment	1961:2061	the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment	1961:2061	Results of this study highlighted a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment.					
35468155	3	51	theme	loaded	566:571	arg1	microparticles					578:591	MLX loaded HPMC microparticles	562:591	MLX loaded HPMC microparticles	562:591	Seventeen formulations of MLX loaded HPMC microparticles were fabricated by the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique.					
35468155	4	52	theme	dependent	815:823	arg1	variables					825:833	the dependent variables	811:833	the dependent variables (responses)	811:845	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	52	theme	dependent	815:823	arg1	size					931:934	mean particle size	917:934	mean particle size (R3)	917:939	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	52	theme	dependent	815:823	arg1	percentage					954:963	in vitro percentage	945:963	in vitro percentage of cumulative drug release (R4)	945:995	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	52	theme	dependent	815:823	arg1	responses					836:844	responses	836:844	responses	836:844	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	52	theme	dependent	815:823	arg1	efficiency					895:904	the entrapment efficiency	880:904	the entrapment efficiency (EE) (R2)	880:914	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	52	theme	dependent	815:823	arg1	yield					868:872	the percent yield	856:872	the percent yield (R1)	856:877	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	14	53	theme	CRC	2049:2051	arg1	treatment					2053:2061	CRC treatment	2049:2061	CRC treatment	2049:2061	Results of this study highlighted a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment.					
35468155	0	54	theme	toxicity	143:150	arg1	study					152:156	in-vivo toxicity study	135:156	in-vivo toxicity study	135:156	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	0	54	theme	toxicity	143:150	arg1	microparticles					27:40	microparticles	27:40	microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study	27:156	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	4	55	theme	independent	786:796	arg1	variables					798:806	the independent variables	782:806	the independent variables	782:806	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	3	56	theme	HPMC	573:576	arg1	microparticles					578:591	MLX loaded HPMC microparticles	562:591	MLX loaded HPMC microparticles	562:591	Seventeen formulations of MLX loaded HPMC microparticles were fabricated by the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique.					
35468155	9	57	theme	drug	1544:1547	arg1	transition					1557:1566	its transition	1553:1566	its transition into an amorphous state after formulation	1553:1608	FTIR analysis showed that the composition of the particles was completely compatible, while XRD analysis confirmed the crystalline nature of the pure drug and its transition into an amorphous state after formulation.					
35468155	9	57	theme	drug	1544:1547	arg1	nature					1525:1530	the crystalline nature	1509:1530	the crystalline nature of the pure drug	1509:1547	FTIR analysis showed that the composition of the particles was completely compatible, while XRD analysis confirmed the crystalline nature of the pure drug and its transition into an amorphous state after formulation.					
35468155	8	58	theme	in	1319:1320	arg1	percentage					1352:1361	the in vitro cumulative drug release percentage	1315:1361	the in vitro cumulative drug release percentage	1315:1361	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	8	58	theme	in	1319:1320	arg1	%					1378:1378	74.25-92.64%	1367:1378	74.25-92.64%	1367:1378	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	14	59	theme	drug-associated	2017:2031	arg1	effects					2038:2044	GIT drug-associated side effects	2013:2044	GIT drug-associated side effects in CRC treatment	2013:2061	Results of this study highlighted a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment.					
35468155	3	60	theme	microparticles	578:591	arg1	formulations					546:557	Seventeen formulations	536:557	Seventeen formulations of MLX loaded HPMC microparticles	536:591	Seventeen formulations of MLX loaded HPMC microparticles were fabricated by the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique.					
35468155	14	61	from	effects	2038:2044	arg1	treatment					2053:2061	CRC treatment	2049:2061	CRC treatment	2049:2061	Results of this study highlighted a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment.					
35468155	9	62	theme	amorphous	1576:1584	arg1	state					1586:1590	an amorphous state	1573:1590	an amorphous state after formulation	1573:1608	FTIR analysis showed that the composition of the particles was completely compatible, while XRD analysis confirmed the crystalline nature of the pure drug and its transition into an amorphous state after formulation.					
35468155	8	63	theme	cumulative	1328:1337	arg1	percentage					1352:1361	the in vitro cumulative drug release percentage	1315:1361	the in vitro cumulative drug release percentage	1315:1361	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	8	63	theme	cumulative	1328:1337	arg1	%					1378:1378	74.25-92.64%	1367:1378	74.25-92.64%	1367:1378	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	13	64	theme	histopathological	1816:1832	arg1	evaluation					1834:1843	The histopathological evaluation	1812:1843	The histopathological evaluation	1812:1843	The histopathological evaluation showed no signs of altered patterns.					
35468155	8	65	theme	release	1344:1350	arg1	percentage					1352:1361	the in vitro cumulative drug release percentage	1315:1361	the in vitro cumulative drug release percentage	1315:1361	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	8	65	theme	release	1344:1350	arg1	%					1378:1378	74.25-92.64%	1367:1378	74.25-92.64%	1367:1378	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	14	66	theme	study	1898:1902	arg1	Results					1882:1888	Results	1882:1888	Results of this study	1882:1902	Results of this study highlighted a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment.					
35468155	4	67	theme	Box	700:702	arg1	design					730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design	680:735	design	730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	0	68	theme	Statistical	71:81	arg1	optimization					83:94	Statistical optimization	71:94	Statistical optimization	71:94	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	0	68	theme	Statistical	71:81	arg1	microparticles					27:40	microparticles	27:40	microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study	27:156	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	3	69	dep	loaded	566:571	arg1	MLX					562:564	MLX	562:564	MLX	562:564	Seventeen formulations of MLX loaded HPMC microparticles were fabricated by the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique.					
35468155	2	70	dep	solubility	466:475	arg1	effects					527:533	associated side effects	511:533	associated side effects	511:533	Nevertheless, its efficiency is limited by poor solubility and gastrointestinal tracts (GIT) associated side effects.					
35468155	4	71	dep	in	945:946	arg1	vitro					948:952	vitro	948:952	vitro	948:952	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	9	72	theme	FTIR	1394:1397	arg1	analysis					1399:1406	FTIR analysis	1394:1406	FTIR analysis	1394:1406	FTIR analysis showed that the composition of the particles was completely compatible, while XRD analysis confirmed the crystalline nature of the pure drug and its transition into an amorphous state after formulation.					
35468155	2	73	theme	associated	511:520	arg1	effects					527:533	associated side effects	511:533	associated side effects	511:533	Nevertheless, its efficiency is limited by poor solubility and gastrointestinal tracts (GIT) associated side effects.					
35468155	14	74	theme	patient	1984:1990	arg1	adherence					1992:2000	patient adherence	1984:2000	patient adherence	1984:2000	Results of this study highlighted a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment.					
35468155	4	75	theme	entrapment	884:893	arg1	EE					907:908	EE	907:908	EE	907:908	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	75	theme	entrapment	884:893	arg1	R2					912:913	R2	912:913	R2	912:913	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	75	theme	entrapment	884:893	arg1	efficiency					895:904	the entrapment efficiency	880:904	the entrapment efficiency (EE) (R2)	880:914	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	5	76	dep	FTIR	1035:1038	arg1	analyses					1059:1066	analyses	1059:1066	analyses	1059:1066	For physicochemical characterization FTIR, XRD, DSC, and SEM analyses were performed.					
35468155	4	77	theme	particle	922:929	arg1	size					931:934	mean particle size	917:934	mean particle size (R3)	917:939	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	77	theme	particle	922:929	arg1	R3					937:938	R3	937:938	R3	937:938	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	1	78	theme	colorectal	393:402	arg1	cancer					404:409	colorectal cancer	393:409	colorectal cancer (CRC)	393:415	The study aimed to fabricate and evaluate Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting because MLX is a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC).					
35468155	1	78	theme	colorectal	393:402	arg1	CRC					412:414	CRC	412:414	CRC	412:414	The study aimed to fabricate and evaluate Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting because MLX is a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC).					
35468155	12	79	theme	toxicity	1731:1738	arg1	study					1740:1744	The toxicity study	1727:1744	The toxicity study in albino rabbits	1727:1762	The toxicity study in albino rabbits showed no toxicity and was found biocompatible.					
35468155	3	80	theme	emulsion	634:641	arg1	ESE					664:666	ESE	664:666	ESE	664:666	Seventeen formulations of MLX loaded HPMC microparticles were fabricated by the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique.					
35468155	3	80	theme	emulsion	634:641	arg1	evaporation					651:661	emulsion solvent evaporation	634:661	the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique	612:677	Seventeen formulations of MLX loaded HPMC microparticles were fabricated by the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique.					
35468155	0	81	theme	colon	46:50	arg1	delivery					61:68	colon targeted delivery	46:68	colon targeted delivery	46:68	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	9	82	theme	particles	1443:1451	arg1	composition					1424:1434	the composition	1420:1434	the composition of the particles	1420:1451	FTIR analysis showed that the composition of the particles was completely compatible, while XRD analysis confirmed the crystalline nature of the pure drug and its transition into an amorphous state after formulation.					
35468155	9	82	theme	particles	1443:1451	arg1	compatible					1468:1477	compatible	1468:1477	compatible	1468:1477	FTIR analysis showed that the composition of the particles was completely compatible, while XRD analysis confirmed the crystalline nature of the pure drug and its transition into an amorphous state after formulation.					
35468155	2	83	theme	poor	461:464	arg1	solubility					466:475	poor solubility	461:475	poor solubility	461:475	Nevertheless, its efficiency is limited by poor solubility and gastrointestinal tracts (GIT) associated side effects.					
35468155	14	84	theme	drug	1935:1938	arg1	system					1949:1954	a standard colonic drug delivery system	1916:1954	a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment	1916:2061	Results of this study highlighted a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment.					
35468155	1	85	theme	potent	313:318	arg1	MLX					304:306	MLX	304:306	MLX	304:306	The study aimed to fabricate and evaluate Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting because MLX is a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC).					
35468155	1	85	theme	potent	313:318	arg1	analgesic					320:328	a potent analgesic	311:328	a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC)	311:415	The study aimed to fabricate and evaluate Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting because MLX is a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC).					
35468155	14	86	theme	standard	1918:1925	arg1	system					1949:1954	a standard colonic drug delivery system	1916:1954	a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment	1916:2061	Results of this study highlighted a standard colonic drug delivery system with the ability to improve patient adherence and reduce GIT drug-associated side effects in CRC treatment.					
35468155	8	87	theme	mean	1271:1274	arg1	size					1285:1288	the mean particle size	1267:1288	the mean particle size	1267:1288	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	8	87	theme	mean	1271:1274	arg1	μm					1307:1308	62.89-284.55 μm	1294:1308	62.89-284.55 μm	1294:1308	However, the mean particle size was 62.89-284.55 μm, and the in vitro cumulative drug release percentage was 74.25-92.64% for 24 hours.					
35468155	4	88	used	used	741:744	arg2	design					730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design	680:735	design	730:735	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	0	89	dep	microparticles	27:40	arg1	study					152:156	in-vivo toxicity study	135:156	in-vivo toxicity study	135:156	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	0	89	dep	microparticles	27:40	arg1	optimization					83:94	Statistical optimization	71:94	Statistical optimization	71:94	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	0	89	dep	microparticles	27:40	arg1	microparticles					27:40	microparticles	27:40	microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study	27:156	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	0	89	dep	microparticles	27:40	arg1	characterization					113:128	physicochemical characterization	97:128	physicochemical characterization	97:128	Developed meloxicam loaded microparticles for colon targeted delivery: Statistical optimization, physicochemical characterization, and in-vivo toxicity study.					
35468155	3	90	theme	evaporation	651:661	arg1	technique					669:677	the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique	612:677	the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique	612:677	Seventeen formulations of MLX loaded HPMC microparticles were fabricated by the oil-in-oil (O/O)/ emulsion solvent evaporation (ESE) technique.					
35468155	11	91	theme	spherical	1706:1714	arg1	particles					1716:1724	dense spherical particles	1700:1724	dense spherical particles	1700:1724	The SEM analysis showed dense spherical particles.					
35468155	1	92	theme	Methylcellulose	238:252	arg1	microparticles					261:274	Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles	201:274	Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting	201:294	The study aimed to fabricate and evaluate Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting because MLX is a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC).					
35468155	9	93	theme	XRD	1486:1488	arg1	analysis					1490:1497	XRD analysis	1486:1497	XRD analysis	1486:1497	FTIR analysis showed that the composition of the particles was completely compatible, while XRD analysis confirmed the crystalline nature of the pure drug and its transition into an amorphous state after formulation.					
35468155	4	94	theme	cumulative	968:977	arg1	R4					993:994	R4	993:994	R4	993:994	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	94	theme	cumulative	968:977	arg1	release					984:990	cumulative drug release	968:990	cumulative drug release (R4)	968:995	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	4	95	theme	combined	762:769	arg1	effects					771:777	the combined effects	758:777	the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4)	758:995	A 3-factor, 3-level Box Behnken (BBD) statistical design was used to estimate the combined effects of the independent variables on the dependent variables (responses), such as the percent yield (R1), the entrapment efficiency (EE) (R2), mean particle size (R3) and in vitro percentage of cumulative drug release (R4).					
35468155	13	96	theme	patterns	1872:1879	arg1	signs					1855:1859	no signs	1852:1859	no signs of altered patterns	1852:1879	The histopathological evaluation showed no signs of altered patterns.					
35468155	1	97	theme	HPMC	255:258	arg1	microparticles					261:274	Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles	201:274	Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting	201:294	The study aimed to fabricate and evaluate Meloxicam (MLX) loaded Hydroxypropyl Methylcellulose (HPMC) microparticles for colon targeting because MLX is a potent analgesic used in the treatment of pain and inflammation associated with colorectal cancer (CRC).					
37285889	5	0	theme	degradation	1055:1065	arg1	rate					1067:1070	degradation rate	1055:1070	degradation rate (68 % remaining weight)	1055:1094	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	2	1	dep	Al2O3	461:465	arg1	%					489:489	Optimal percentage 5 %	468:489	Optimal percentage 5 %	468:489	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	7	2	dep	viability	1300:1308	arg1	the					1296:1298	the	1296:1298	the	1296:1298	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	5	3	theme	uncoated	1112:1119	arg1	scaffolds					1121:1129	the uncoated scaffolds	1108:1129	the uncoated scaffolds	1108:1129	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	0	4	theme	coating	81:87	arg1	performance					37:47	Physical, mechanical, and biological performance	0:47	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating	0:87	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	7	5	theme	alkaline	1365:1372	arg1	phosphatase					1374:1384	alkaline phosphatase	1365:1384	alkaline phosphatase	1365:1384	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	7	6	theme	activity	1393:1400	arg1	secretion					1352:1360	secretion	1352:1360	secretion	1352:1360	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	7	6	theme	activity	1393:1400	arg1	candidate					1441:1449	a suitable candidate	1430:1449	a suitable candidate for use in bone tissue engineering	1430:1484	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	5	7	theme	coated	868:873	arg1	scaffolds					875:883	The coated scaffolds	864:883	The coated scaffolds	864:883	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	5	8	dep	hydrophilicity	1008:1021	arg1	32.69°					1030:1035	32.69°	1030:1035	32.69°	1030:1035	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	7	9	theme	PMA	1259:1261	arg1	scaffold					1263:1270	the PMA scaffold	1255:1270	the PMA scaffold	1255:1270	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	4	10	theme	uncoated	844:851	arg1	scaffolds					853:861	the uncoated scaffolds	840:861	the uncoated scaffolds	840:861	The SEM results of the coated scaffolds showed homogeneous three-dimensional structures with interconnected pores compared to the uncoated scaffolds.					
37285889	7	11	theme	MG-63	1331:1335	arg1	cells					1337:1341	MG-63 cells	1331:1341	MG-63 cells	1331:1341	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	4	12	with	structures	791:800	arg1	pores					822:826	interconnected pores	807:826	interconnected pores	807:826	The SEM results of the coated scaffolds showed homogeneous three-dimensional structures with interconnected pores compared to the uncoated scaffolds.					
37285889	7	13	theme	suitable	1432:1439	arg1	secretion					1352:1360	secretion	1352:1360	secretion	1352:1360	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	7	13	theme	suitable	1432:1439	arg1	candidate					1441:1449	a suitable candidate	1430:1449	a suitable candidate for use in bone tissue engineering	1430:1484	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	3	14	theme	Cs	675:676	arg1	presence					663:670	the presence	659:670	the presence of Cs and MWCNTs in the coated scaffolds	659:711	Structural analyses such as XRD and ATR-FTIR confirmed the presence of Cs and MWCNTs in the coated scaffolds.					
37285889	2	15	theme	carbon	546:551	arg1	MWCNTs					564:569	MWCNTs	564:569	MWCNTs	564:569	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	15	theme	carbon	546:551	arg1	nanotubes					553:561	chitosan (Cs)/multi-walled carbon nanotubes	519:561	chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs)	519:570	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	5	16	dep	increase	898:905	arg1	up					932:933	up	932:933	up to 16.1 MPa	932:945	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	5	16	dep	increase	898:905	arg1	up					973:974	up	973:974	up to 40.83 MPa	973:987	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	5	17	from	modulus	964:970	arg1	strength					922:929	compression strength	910:929	compression strength	910:929	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	5	17	from	modulus	964:970	arg1	rate					1067:1070	degradation rate	1055:1070	degradation rate (68 % remaining weight)	1055:1094	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	0	18	from	application	160:170	arg1	engineering					187:197	bone tissue engineering	175:197	bone tissue engineering	175:197	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	0	19	theme	3D	129:130	arg1	scaffold					140:147	the polycaprolactone-based 3D printed scaffold	102:147	the polycaprolactone-based 3D printed scaffold	102:147	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	4	20	theme	SEM	718:720	arg1	results					722:728	The SEM results	714:728	The SEM results of the coated scaffolds	714:752	The SEM results of the coated scaffolds showed homogeneous three-dimensional structures with interconnected pores compared to the uncoated scaffolds.					
37285889	2	21	theme	/magnetic	399:407	arg1	PMA					493:495	PMA	493:495	PMA	493:495	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	21	theme	/magnetic	399:407	arg1	nanowire					451:458	mesoporous bioactive glass (MMBG)/alumina nanowire	409:458	polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA)	377:496	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	21	theme	/magnetic	399:407	arg1	Al2O3					461:465	Al2O3	461:465	Al2O3	461:465	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	0	22	theme	polycaprolactone-based	106:127	arg1	scaffold					140:147	the polycaprolactone-based 3D printed scaffold	102:147	the polycaprolactone-based 3D printed scaffold	102:147	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	2	23	theme	Optimal	468:474	arg1	%					489:489	Optimal percentage 5 %	468:489	Optimal percentage 5 %	468:489	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	0	24	theme	Physical	0:7	arg1	performance					37:47	Physical, mechanical, and biological performance	0:47	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating	0:87	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	3	25	from	presence	663:670	arg1	scaffolds					703:711	the coated scaffolds	692:711	the coated scaffolds	692:711	Structural analyses such as XRD and ATR-FTIR confirmed the presence of Cs and MWCNTs in the coated scaffolds.					
37285889	2	26	theme	percentage	476:485	arg1	%					489:489	Optimal percentage 5 %	468:489	Optimal percentage 5 %	468:489	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	5	27	from	increase	898:905	arg1	strength					922:929	compression strength	910:929	compression strength	910:929	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	5	27	from	increase	898:905	arg1	rate					1067:1070	degradation rate	1055:1070	degradation rate (68 % remaining weight)	1055:1094	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	2	28	theme	/alumina	442:449	arg1	PMA					493:495	PMA	493:495	PMA	493:495	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	28	theme	/alumina	442:449	arg1	nanowire					451:458	mesoporous bioactive glass (MMBG)/alumina nanowire	409:458	polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA)	377:496	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	28	theme	/alumina	442:449	arg1	Al2O3					461:465	Al2O3	461:465	Al2O3	461:465	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	5	29	dep	rate	1067:1070	arg1	%					1076:1076	68 %	1073:1076	68 % remaining weight	1073:1093	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	4	30	theme	interconnected	807:820	arg1	pores					822:826	interconnected pores	807:826	interconnected pores	807:826	The SEM results of the coated scaffolds showed homogeneous three-dimensional structures with interconnected pores compared to the uncoated scaffolds.					
37285889	7	31	theme	Ca	1390:1391	arg1	activity					1393:1400	Ca activity	1390:1400	Ca activity	1390:1400	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	5	32	theme	compression	910:920	arg1	strength					922:929	compression strength	910:929	compression strength	910:929	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	6	33	theme	XRD	1236:1238	arg1	tests					1240:1244	XRD tests	1236:1244	XRD tests	1236:1244	The increase in apatite formation in the scaffold coated with Cs/MWCNTs was confirmed by SEM, EDAX, and XRD tests.					
37285889	3	34	theme	Structural	604:613	arg1	ATR-FTIR					640:647	ATR-FTIR	640:647	ATR-FTIR	640:647	Structural analyses such as XRD and ATR-FTIR confirmed the presence of Cs and MWCNTs in the coated scaffolds.					
37285889	3	34	theme	Structural	604:613	arg1	analyses					615:622	Structural analyses	604:622	Structural analyses such as XRD and ATR-FTIR	604:647	Structural analyses such as XRD and ATR-FTIR confirmed the presence of Cs and MWCNTs in the coated scaffolds.					
37285889	3	34	theme	Structural	604:613	arg1	XRD					632:634	XRD	632:634	XRD	632:634	Structural analyses such as XRD and ATR-FTIR confirmed the presence of Cs and MWCNTs in the coated scaffolds.					
37285889	3	35	theme	coated	696:701	arg1	scaffolds					703:711	the coated scaffolds	692:711	the coated scaffolds	692:711	Structural analyses such as XRD and ATR-FTIR confirmed the presence of Cs and MWCNTs in the coated scaffolds.					
37285889	2	36	theme	chitosan	519:526	arg1	MWCNTs					564:569	MWCNTs	564:569	MWCNTs	564:569	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	36	theme	chitosan	519:526	arg1	nanotubes					553:561	chitosan (Cs)/multi-walled carbon nanotubes	519:561	chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs)	519:570	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	37	theme	MMBG	437:440	arg1	PMA					493:495	PMA	493:495	PMA	493:495	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	37	theme	MMBG	437:440	arg1	nanowire					451:458	mesoporous bioactive glass (MMBG)/alumina nanowire	409:458	polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA)	377:496	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	37	theme	MMBG	437:440	arg1	Al2O3					461:465	Al2O3	461:465	Al2O3	461:465	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	3	38	theme	MWCNTs	682:687	arg1	presence					663:670	the presence	659:670	the presence of Cs and MWCNTs in the coated scaffolds	659:711	Structural analyses such as XRD and ATR-FTIR confirmed the presence of Cs and MWCNTs in the coated scaffolds.					
37285889	0	39	theme	printed	132:138	arg1	scaffold					140:147	the polycaprolactone-based 3D printed scaffold	102:147	the polycaprolactone-based 3D printed scaffold	102:147	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	2	40	theme	glass	430:434	arg1	PMA					493:495	PMA	493:495	PMA	493:495	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	40	theme	glass	430:434	arg1	nanowire					451:458	mesoporous bioactive glass (MMBG)/alumina nanowire	409:458	polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA)	377:496	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	40	theme	glass	430:434	arg1	Al2O3					461:465	Al2O3	461:465	Al2O3	461:465	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	0	41	theme	biological	26:35	arg1	performance					37:47	Physical, mechanical, and biological performance	0:47	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating	0:87	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	0	42	theme	Potential	150:158	arg1	application					160:170	Potential application	150:170	Potential application in bone tissue engineering	150:197	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	2	43	theme	bioactive	420:428	arg1	PMA					493:495	PMA	493:495	PMA	493:495	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	43	theme	bioactive	420:428	arg1	nanowire					451:458	mesoporous bioactive glass (MMBG)/alumina nanowire	409:458	polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA)	377:496	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	43	theme	bioactive	420:428	arg1	Al2O3					461:465	Al2O3	461:465	Al2O3	461:465	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	1	44	theme	composite	221:229	arg1	scaffolds					231:239	composite scaffolds	221:239	composite scaffolds	221:239	Recently, coating on composite scaffolds has attracted many researchers' attention to improve scaffolds' properties.					
37285889	7	45	from	use	1455:1457	arg1	engineering					1474:1484	bone tissue engineering	1462:1484	bone tissue engineering	1462:1484	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	4	46	theme	scaffolds	744:752	arg1	results					722:728	The SEM results	714:728	The SEM results of the coated scaffolds	714:752	The SEM results of the coated scaffolds showed homogeneous three-dimensional structures with interconnected pores compared to the uncoated scaffolds.					
37285889	2	47	theme	mesoporous	409:418	arg1	PMA					493:495	PMA	493:495	PMA	493:495	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	47	theme	mesoporous	409:418	arg1	nanowire					451:458	mesoporous bioactive glass (MMBG)/alumina nanowire	409:458	polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA)	377:496	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	47	theme	mesoporous	409:418	arg1	Al2O3					461:465	Al2O3	461:465	Al2O3	461:465	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	48	theme	/multi-walled	532:544	arg1	MWCNTs					564:569	MWCNTs	564:569	MWCNTs	564:569	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	2	48	theme	/multi-walled	532:544	arg1	nanotubes					553:561	chitosan (Cs)/multi-walled carbon nanotubes	519:561	chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs)	519:570	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	5	49	from	hydrophilicity	1008:1021	arg1	strength					922:929	compression strength	910:929	compression strength	910:929	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	5	49	from	hydrophilicity	1008:1021	arg1	rate					1067:1070	degradation rate	1055:1070	degradation rate (68 % remaining weight)	1055:1094	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	4	50	theme	three-dimensional	773:789	arg1	structures					791:800	homogeneous three-dimensional structures	761:800	homogeneous three-dimensional structures with interconnected pores	761:826	The SEM results of the coated scaffolds showed homogeneous three-dimensional structures with interconnected pores compared to the uncoated scaffolds.					
37285889	5	51	from	decrease	1043:1050	arg1	strength					922:929	compression strength	910:929	compression strength	910:929	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	5	51	from	decrease	1043:1050	arg1	rate					1067:1070	degradation rate	1055:1070	degradation rate (68 % remaining weight)	1055:1094	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	7	52	theme	phosphatase	1374:1384	arg1	secretion					1352:1360	secretion	1352:1360	secretion	1352:1360	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	7	52	theme	phosphatase	1374:1384	arg1	candidate					1441:1449	a suitable candidate	1430:1449	a suitable candidate for use in bone tissue engineering	1430:1484	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	7	53	dep	secretion	1352:1360	arg1	more					1347:1350	more	1347:1350	more	1347:1350	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	4	54	theme	homogeneous	761:771	arg1	structures					791:800	homogeneous three-dimensional structures	761:800	homogeneous three-dimensional structures with interconnected pores	761:826	The SEM results of the coated scaffolds showed homogeneous three-dimensional structures with interconnected pores compared to the uncoated scaffolds.					
37285889	1	55	from	coating	210:216	arg1	scaffolds					231:239	composite scaffolds	221:239	composite scaffolds	221:239	Recently, coating on composite scaffolds has attracted many researchers' attention to improve scaffolds' properties.					
37285889	2	56	theme	printed	340:346	arg1	scaffold					348:355	a 3D printed scaffold	335:355	a 3D printed scaffold	335:355	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	0	57	theme	tissue	180:185	arg1	engineering					187:197	bone tissue engineering	175:197	bone tissue engineering	175:197	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	5	58	theme	improved	991:998	arg1	hydrophilicity					1008:1021	improved surface hydrophilicity	991:1021	improved surface hydrophilicity (up to 32.69°)	991:1036	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	6	59	theme	apatite	1148:1154	arg1	formation					1156:1164	apatite formation	1148:1164	apatite formation	1148:1164	The increase in apatite formation in the scaffold coated with Cs/MWCNTs was confirmed by SEM, EDAX, and XRD tests.					
37285889	2	60	theme	3D	337:338	arg1	scaffold					348:355	a 3D printed scaffold	335:355	a 3D printed scaffold	335:355	In this research, a 3D printed scaffold was fabricated from polycaprolactone (PCL)/magnetic mesoporous bioactive glass (MMBG)/alumina nanowire (Al2O3, Optimal percentage 5 %) (PMA) and then coated with chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) by an immersion coating method.					
37285889	6	61	from	increase	1136:1143	arg1	scaffold					1173:1180	the scaffold	1169:1180	the scaffold coated with Cs/MWCNTs	1169:1202	The increase in apatite formation in the scaffold coated with Cs/MWCNTs was confirmed by SEM, EDAX, and XRD tests.					
37285889	6	61	from	increase	1136:1143	arg1	formation					1156:1164	apatite formation	1148:1164	apatite formation	1148:1164	The increase in apatite formation in the scaffold coated with Cs/MWCNTs was confirmed by SEM, EDAX, and XRD tests.					
37285889	7	62	theme	tissue	1467:1472	arg1	engineering					1474:1484	bone tissue engineering	1462:1484	bone tissue engineering	1462:1484	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	0	63	theme	bone	175:178	arg1	engineering					187:197	bone tissue engineering	175:197	bone tissue engineering	175:197	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	5	64	theme	surface	1000:1006	arg1	hydrophilicity					1008:1021	improved surface hydrophilicity	991:1021	improved surface hydrophilicity (up to 32.69°)	991:1036	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	5	65	theme	compressive	952:962	arg1	modulus					964:970	compressive modulus	952:970	compressive modulus	952:970	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	3	66	attach	presence	663:670	arg2	MWCNTs					682:687	MWCNTs	682:687	MWCNTs	682:687	Structural analyses such as XRD and ATR-FTIR confirmed the presence of Cs and MWCNTs in the coated scaffolds.					
37285889	3	66	attach	presence	663:670	arg2	Cs					675:676	Cs	675:676	Cs	675:676	Structural analyses such as XRD and ATR-FTIR confirmed the presence of Cs and MWCNTs in the coated scaffolds.					
37285889	3	66	attach	presence	663:670	arg1	scaffolds					703:711	the coated scaffolds	692:711	the coated scaffolds	692:711	Structural analyses such as XRD and ATR-FTIR confirmed the presence of Cs and MWCNTs in the coated scaffolds.					
37285889	5	67	dep	32.69°	1030:1035	arg1	to					1027:1028	to	1027:1028	to	1027:1028	The coated scaffolds exhibited an increase in compression strength (up to 16.1 MPa) and compressive modulus (up to 40.83 MPa), improved surface hydrophilicity (up to 32.69°), and decrease in degradation rate (68 % remaining weight) compared to the uncoated scaffolds.					
37285889	0	68	theme	nanocomposite	67:79	arg1	coating					81:87	chitosan-based nanocomposite coating	52:87	chitosan-based nanocomposite coating	52:87	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	7	69	theme	cells	1337:1341	arg1	viability					1300:1308	viability	1300:1308	viability	1300:1308	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	7	69	theme	cells	1337:1341	arg1	proliferation					1314:1326	proliferation	1314:1326	proliferation	1314:1326	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37285889	4	70	theme	coated	737:742	arg1	scaffolds					744:752	the coated scaffolds	733:752	the coated scaffolds	733:752	The SEM results of the coated scaffolds showed homogeneous three-dimensional structures with interconnected pores compared to the uncoated scaffolds.					
37285889	1	71	theme	many	255:258	arg1	researchers					260:270	many researchers'	255:271	many researchers' attention	255:281	Recently, coating on composite scaffolds has attracted many researchers' attention to improve scaffolds' properties.					
37285889	0	72	theme	chitosan-based	52:65	arg1	coating					81:87	chitosan-based nanocomposite coating	52:87	chitosan-based nanocomposite coating	52:87	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	0	73	theme	mechanical	10:19	arg1	performance					37:47	Physical, mechanical, and biological performance	0:47	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating	0:87	Physical, mechanical, and biological performance of chitosan-based nanocomposite coating deposited on the polycaprolactone-based 3D printed scaffold: Potential application in bone tissue engineering.					
37285889	7	74	theme	bone	1462:1465	arg1	engineering					1474:1484	bone tissue engineering	1462:1484	bone tissue engineering	1462:1484	Coating the PMA scaffold with Cs/MWCNTs leads to the viability and proliferation of MG-63 cells and more secretion of alkaline phosphatase and Ca activity, which can be introduced as a suitable candidate for use in bone tissue engineering.					
37110540	8	0	theme	choline	1192:1198	arg1	acid/1,4-butanediol					1216:1234	choline chloride/oxalic acid/1,4-butanediol	1192:1234	choline chloride/oxalic acid/1,4-butanediol	1192:1234	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	0	theme	choline	1192:1198	arg1	ChCl/OA/BD					1237:1246	ChCl/OA/BD	1237:1246	ChCl/OA/BD	1237:1246	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	5	1	theme	high	756:759	arg1	concentration					761:773	a high concentration	754:773	a high concentration of zinc chloride aqueous solution (65-85 w%)	754:818	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	4	2	theme	w	548:548	arg1	%					549:549	30-55 w%	542:549	30-55 w%	542:549	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	2	theme	w	548:548	arg1	solution					532:539	zinc chloride aqueous solution	510:539	zinc chloride aqueous solution (30-55 w%)	510:550	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	3	theme	w	608:608	arg1	%					628:628	selectivity = 89%	612:628	selectivity = 89%	612:628	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	3	theme	w	608:608	arg1	%					609:609	30.4 w%	603:609	30.4 w% (selectivity = 89%)	603:629	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	3	theme	w	608:608	arg1	xylose					634:639	xylose	634:639	xylose obtained with a solid residue of the composite of cellulose and lignin	634:710	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	8	4	theme	deep	1163:1166	arg1	DES					1186:1188	DES	1186:1188	DES	1186:1188	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	4	theme	deep	1163:1166	arg1	solvent					1177:1183	a ternary deep eutectic solvent	1153:1183	a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD)	1153:1247	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	0	5	theme	Mild	79:82	arg1	Conditions					84:93	Mild Conditions	79:93	Mild Conditions	79:93	Fast and Selective Degradation of Biomass for Xylose, Glucose and Lignin under Mild Conditions.					
37110540	8	6	dep	solvent	1177:1183	arg1	acid/1,4-butanediol					1216:1234	choline chloride/oxalic acid/1,4-butanediol	1192:1234	choline chloride/oxalic acid/1,4-butanediol	1192:1234	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	6	dep	solvent	1177:1183	arg1	ChCl/OA/BD					1237:1246	ChCl/OA/BD	1237:1246	ChCl/OA/BD	1237:1246	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	6	7	theme	xylose	951:956	arg1	%					963:963	97%	961:963	97%	961:963	Combining the two steps, the total yield of xylose is 97%, while glucose is 95%.					
37110540	6	7	theme	xylose	951:956	arg1	yield					942:946	the total yield	932:946	the total yield of xylose	932:956	Combining the two steps, the total yield of xylose is 97%, while glucose is 95%.					
37110540	3	8	theme	two-step	342:349	arg1	process					351:357	a two-step process	340:357	a two-step process	340:357	Therefore, a two-step process has been developed to degrade corncob into xylose and glucose under mild conditions.					
37110540	4	9	theme	=	624:624	arg1	%					628:628	selectivity = 89%	612:628	selectivity = 89%	612:628	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	9	theme	=	624:624	arg1	%					609:609	30.4 w%	603:609	30.4 w% (selectivity = 89%)	603:629	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	9	theme	=	624:624	arg1	xylose					634:639	xylose	634:639	xylose obtained with a solid residue of the composite of cellulose and lignin	634:710	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	5	10	theme	glucose	882:888	arg1	%					876:876	selectivity = 92%	860:876	selectivity = 92%	860:876	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	10	theme	glucose	882:888	arg1	%					857:857	29.4 w%	851:857	29.4 w% (selectivity = 92%) of glucose	851:888	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	10	theme	glucose	882:888	arg1	glucose					882:888	glucose	882:888	glucose	882:888	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	2	11	theme	cellulose	267:275	arg1	challenge					318:326	a challenge	316:326	a challenge	316:326	However, selective degradation of hemicellulose and cellulose with the production of lignin is still a challenge.					
37110540	2	11	theme	cellulose	267:275	arg1	degradation					234:244	selective degradation	224:244	selective degradation of hemicellulose and cellulose with the production of lignin	224:305	However, selective degradation of hemicellulose and cellulose with the production of lignin is still a challenge.					
37110540	5	12	theme	solid	723:727	arg1	residue					729:735	the solid residue	719:735	the solid residue	719:735	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	6	13	theme	total	936:940	arg1	%					963:963	97%	961:963	97%	961:963	Combining the two steps, the total yield of xylose is 97%, while glucose is 95%.					
37110540	6	13	theme	total	936:940	arg1	yield					942:946	the total yield	932:946	the total yield of xylose	932:956	Combining the two steps, the total yield of xylose is 97%, while glucose is 95%.					
37110540	5	14	theme	solution	800:807	arg1	concentration					761:773	a high concentration	754:773	a high concentration of zinc chloride aqueous solution (65-85 w%)	754:818	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	7	15	theme	high	1001:1004	arg1	lignin					1011:1016	high pure lignin	1001:1016	high pure lignin	1001:1016	In addition, high pure lignin can be obtained simultaneously, which was confirmed using HSQC studies.					
37110540	0	16	theme	Fast	0:3	arg1	Degradation					19:29	Fast and Selective Degradation	0:29	Fast and Selective Degradation of Biomass for Xylose, Glucose and Lignin under Mild Conditions.	0:94	Fast and Selective Degradation of Biomass for Xylose, Glucose and Lignin under Mild Conditions.					
37110540	4	17	theme	aqueous	524:530	arg1	%					549:549	30-55 w%	542:549	30-55 w%	542:549	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	17	theme	aqueous	524:530	arg1	solution					532:539	zinc chloride aqueous solution	510:539	zinc chloride aqueous solution (30-55 w%)	510:550	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	5	18	theme	selectivity	860:870	arg1	%					876:876	selectivity = 92%	860:876	selectivity = 92%	860:876	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	18	theme	selectivity	860:870	arg1	%					857:857	29.4 w%	851:857	29.4 w% (selectivity = 92%) of glucose	851:888	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	18	theme	selectivity	860:870	arg1	glucose					882:888	glucose	882:888	glucose	882:888	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	2	19	theme	lignin	300:305	arg1	production					286:295	the production	282:295	the production of lignin	282:305	However, selective degradation of hemicellulose and cellulose with the production of lignin is still a challenge.					
37110540	4	20	theme	chloride	515:522	arg1	%					549:549	30-55 w%	542:549	30-55 w%	542:549	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	20	theme	chloride	515:522	arg1	solution					532:539	zinc chloride aqueous solution	510:539	zinc chloride aqueous solution (30-55 w%)	510:550	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	5	21	theme	aqueous	792:798	arg1	solution					800:807	zinc chloride aqueous solution	778:807	zinc chloride aqueous solution (65-85 w%)	778:818	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	21	theme	aqueous	792:798	arg1	%					817:817	65-85 w%	810:817	65-85 w%	810:817	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	4	22	theme	xylose	634:639	arg1	min					594:596	8-12 min	589:596	8-12 min	589:596	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	22	theme	xylose	634:639	arg1	%					628:628	selectivity = 89%	612:628	selectivity = 89%	612:628	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	22	theme	xylose	634:639	arg1	time					583:586	a short reaction time	566:586	a short reaction time (8-12 min)	566:597	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	22	theme	xylose	634:639	arg1	%					609:609	30.4 w%	603:609	30.4 w% (selectivity = 89%)	603:629	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	22	theme	xylose	634:639	arg1	xylose					634:639	xylose	634:639	xylose obtained with a solid residue of the composite of cellulose and lignin	634:710	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	1	23	theme	key	180:182	arg1	technology					184:193	the key technology	176:193	the key technology in green chemistry	176:212	The conversion of lignocellulose into valuable chemicals has been recognized as the key technology in green chemistry.					
37110540	1	23	theme	key	180:182	arg1	conversion					100:109	The conversion	96:109	The conversion of lignocellulose into valuable chemicals	96:151	The conversion of lignocellulose into valuable chemicals has been recognized as the key technology in green chemistry.					
37110540	0	24	theme	Selective	9:17	arg1	Degradation					19:29	Fast and Selective Degradation	0:29	Fast and Selective Degradation of Biomass for Xylose, Glucose and Lignin under Mild Conditions.	0:94	Fast and Selective Degradation of Biomass for Xylose, Glucose and Lignin under Mild Conditions.					
37110540	4	25	theme	solid	657:661	arg1	residue					663:669	a solid residue	655:669	a solid residue of the composite of cellulose and lignin	655:710	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	25	theme	solid	657:661	arg1	composite					678:686	the composite	674:686	the composite of cellulose and lignin	674:710	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	5	26	theme	=	872:872	arg1	%					876:876	selectivity = 92%	860:876	selectivity = 92%	860:876	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	26	theme	=	872:872	arg1	%					857:857	29.4 w%	851:857	29.4 w% (selectivity = 92%) of glucose	851:888	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	26	theme	=	872:872	arg1	glucose					882:888	glucose	882:888	glucose	882:888	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	27	theme	w	816:816	arg1	solution					800:807	zinc chloride aqueous solution	778:807	zinc chloride aqueous solution (65-85 w%)	778:818	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	27	theme	w	816:816	arg1	%					817:817	65-85 w%	810:817	65-85 w%	810:817	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	0	28	theme	Biomass	34:40	arg1	Degradation					19:29	Fast and Selective Degradation	0:29	Fast and Selective Degradation of Biomass for Xylose, Glucose and Lignin under Mild Conditions.	0:94	Fast and Selective Degradation of Biomass for Xylose, Glucose and Lignin under Mild Conditions.					
37110540	4	29	theme	solution	532:539	arg1	concentration					493:505	the lower concentration	483:505	the lower concentration of zinc chloride aqueous solution (30-55 w%)	483:550	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	8	30	theme	ternary	1155:1161	arg1	DES					1186:1188	DES	1186:1188	DES	1186:1188	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	30	theme	ternary	1155:1161	arg1	solvent					1177:1183	a ternary deep eutectic solvent	1153:1183	a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD)	1153:1247	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	4	31	theme	cellulose	691:699	arg1	cellulose					691:699	cellulose	691:699	cellulose	691:699	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	31	theme	cellulose	691:699	arg1	composite					678:686	the composite	674:686	the composite of cellulose and lignin	674:710	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	31	theme	cellulose	691:699	arg1	lignin					705:710	lignin	705:710	lignin	705:710	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	3	32	theme	mild	427:430	arg1	conditions					432:441	mild conditions	427:441	mild conditions	427:441	Therefore, a two-step process has been developed to degrade corncob into xylose and glucose under mild conditions.					
37110540	2	33	with	degradation	234:244	arg1	production					286:295	the production	282:295	the production of lignin	282:305	However, selective degradation of hemicellulose and cellulose with the production of lignin is still a challenge.					
37110540	8	34	used	used	1258:1261	arg2	DES					1186:1188	DES	1186:1188	DES	1186:1188	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	34	used	used	1258:1261	arg2	solvent					1177:1183	a ternary deep eutectic solvent	1153:1183	a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD)	1153:1247	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	35	theme	solid	1111:1115	arg1	reaction					1143:1150	the first-step reaction	1128:1150	the first-step reaction	1128:1150	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	35	theme	solid	1111:1115	arg1	residue					1117:1123	the solid residue	1107:1123	the solid residue of the first-step reaction	1107:1150	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	36	dep	cellulose	1279:1287	arg1	obtained					1370:1377	obtained	1370:1377	were obtained	1365:1377	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	36	dep	cellulose	1279:1287	arg1	the					1275:1277	the	1275:1277	the	1275:1277	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	4	37	theme	reaction	574:581	arg1	time					583:586	a short reaction time	566:586	a short reaction time (8-12 min)	566:597	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	37	theme	reaction	574:581	arg1	min					594:596	8-12 min	589:596	8-12 min	589:596	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	38	theme	lower	487:491	arg1	concentration					493:505	the lower concentration	483:505	the lower concentration of zinc chloride aqueous solution (30-55 w%)	483:550	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	9	39	theme	simple	1407:1412	arg1	method					1414:1419	a simple method	1405:1419	a simple method to disassemble the lignocellulose for monosaccharides, lignin, and cellulose	1405:1496	Furthermore, it provides a simple method to disassemble the lignocellulose for monosaccharides, lignin, and cellulose.					
37110540	8	40	theme	eutectic	1168:1175	arg1	DES					1186:1188	DES	1186:1188	DES	1186:1188	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	40	theme	eutectic	1168:1175	arg1	solvent					1177:1183	a ternary deep eutectic solvent	1153:1183	a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD)	1153:1247	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	4	41	theme	lignin	705:710	arg1	cellulose					691:699	cellulose	691:699	cellulose	691:699	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	41	theme	lignin	705:710	arg1	composite					678:686	the composite	674:686	the composite of cellulose and lignin	674:710	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	41	theme	lignin	705:710	arg1	lignin					705:710	lignin	705:710	lignin	705:710	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	42	theme	selectivity	612:622	arg1	%					628:628	selectivity = 89%	612:628	selectivity = 89%	612:628	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	42	theme	selectivity	612:622	arg1	%					609:609	30.4 w%	603:609	30.4 w% (selectivity = 89%)	603:629	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	42	theme	selectivity	612:622	arg1	xylose					634:639	xylose	634:639	xylose obtained with a solid residue of the composite of cellulose and lignin	634:710	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	43	theme	zinc	510:513	arg1	%					549:549	30-55 w%	542:549	30-55 w%	542:549	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	43	theme	zinc	510:513	arg1	solution					532:539	zinc chloride aqueous solution	510:539	zinc chloride aqueous solution (30-55 w%)	510:550	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	5	44	theme	w	856:856	arg1	%					876:876	selectivity = 92%	860:876	selectivity = 92%	860:876	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	44	theme	w	856:856	arg1	%					857:857	29.4 w%	851:857	29.4 w% (selectivity = 92%) of glucose	851:888	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	44	theme	w	856:856	arg1	glucose					882:888	glucose	882:888	glucose	882:888	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	7	45	theme	pure	1006:1009	arg1	lignin					1011:1016	high pure lignin	1001:1016	high pure lignin	1001:1016	In addition, high pure lignin can be obtained simultaneously, which was confirmed using HSQC studies.					
37110540	5	46	theme	zinc	778:781	arg1	solution					800:807	zinc chloride aqueous solution	778:807	zinc chloride aqueous solution (65-85 w%)	778:818	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	46	theme	zinc	778:781	arg1	%					817:817	65-85 w%	810:817	65-85 w%	810:817	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	7	47	theme	HSQC	1076:1079	arg1	studies					1081:1087	HSQC studies	1076:1087	HSQC studies	1076:1087	In addition, high pure lignin can be obtained simultaneously, which was confirmed using HSQC studies.					
37110540	8	48	theme	reaction	1143:1150	arg1	reaction					1143:1150	the first-step reaction	1128:1150	the first-step reaction	1128:1150	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	48	theme	reaction	1143:1150	arg1	residue					1117:1123	the solid residue	1107:1123	the solid residue of the first-step reaction	1107:1150	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	2	49	theme	hemicellulose	249:261	arg1	challenge					318:326	a challenge	316:326	a challenge	316:326	However, selective degradation of hemicellulose and cellulose with the production of lignin is still a challenge.					
37110540	2	49	theme	hemicellulose	249:261	arg1	degradation					234:244	selective degradation	224:244	selective degradation of hemicellulose and cellulose with the production of lignin	224:305	However, selective degradation of hemicellulose and cellulose with the production of lignin is still a challenge.					
37110540	1	50	from	technology	184:193	arg1	chemistry					204:212	green chemistry	198:212	green chemistry	198:212	The conversion of lignocellulose into valuable chemicals has been recognized as the key technology in green chemistry.					
37110540	5	51	theme	chloride	783:790	arg1	solution					800:807	zinc chloride aqueous solution	778:807	zinc chloride aqueous solution (65-85 w%)	778:818	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	5	51	theme	chloride	783:790	arg1	%					817:817	65-85 w%	810:817	65-85 w%	810:817	Next, the solid residue was treated with a high concentration of zinc chloride aqueous solution (65-85 w%) at 95 °C for about 10 min, and 29.4 w% (selectivity = 92%) of glucose can be obtained.					
37110540	8	52	theme	first-step	1132:1141	arg1	reaction					1143:1150	the first-step reaction	1128:1150	the first-step reaction	1128:1150	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	1	53	theme	lignocellulose	114:127	arg1	technology					184:193	the key technology	176:193	the key technology in green chemistry	176:212	The conversion of lignocellulose into valuable chemicals has been recognized as the key technology in green chemistry.					
37110540	1	53	theme	lignocellulose	114:127	arg1	conversion					100:109	The conversion	96:109	The conversion of lignocellulose into valuable chemicals	96:151	The conversion of lignocellulose into valuable chemicals has been recognized as the key technology in green chemistry.					
37110540	1	54	theme	green	198:202	arg1	chemistry					204:212	green chemistry	198:212	green chemistry	198:212	The conversion of lignocellulose into valuable chemicals has been recognized as the key technology in green chemistry.					
37110540	4	55	theme	composite	678:686	arg1	residue					663:669	a solid residue	655:669	a solid residue of the composite of cellulose and lignin	655:710	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	55	theme	composite	678:686	arg1	composite					678:686	the composite	674:686	the composite of cellulose and lignin	674:710	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	56	theme	short	568:572	arg1	time					583:586	a short reaction time	566:586	a short reaction time (8-12 min)	566:597	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	4	56	theme	short	568:572	arg1	min					594:596	8-12 min	589:596	8-12 min	589:596	At first, the corncob was treated with the lower concentration of zinc chloride aqueous solution (30-55 w%) at 95 °C with a short reaction time (8-12 min) and 30.4 w% (selectivity = 89%) of xylose obtained with a solid residue of the composite of cellulose and lignin.					
37110540	8	57	theme	chloride/oxalic	1200:1214	arg1	acid/1,4-butanediol					1216:1234	choline chloride/oxalic acid/1,4-butanediol	1192:1234	choline chloride/oxalic acid/1,4-butanediol	1192:1234	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	57	theme	chloride/oxalic	1200:1214	arg1	ChCl/OA/BD					1237:1246	ChCl/OA/BD	1237:1246	ChCl/OA/BD	1237:1246	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	2	58	theme	selective	224:232	arg1	challenge					318:326	a challenge	316:326	a challenge	316:326	However, selective degradation of hemicellulose and cellulose with the production of lignin is still a challenge.					
37110540	2	58	theme	selective	224:232	arg1	degradation					234:244	selective degradation	224:244	selective degradation of hemicellulose and cellulose with the production of lignin	224:305	However, selective degradation of hemicellulose and cellulose with the production of lignin is still a challenge.					
37110540	1	59	theme	valuable	134:141	arg1	chemicals					143:151	valuable chemicals	134:151	valuable chemicals	134:151	The conversion of lignocellulose into valuable chemicals has been recognized as the key technology in green chemistry.					
37110540	8	60	theme	high-quality	1317:1328	arg1	Re-C					1341:1344	Re-C	1341:1344	Re-C	1341:1344	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37110540	8	60	theme	high-quality	1317:1328	arg1	cellulose					1330:1338	high-quality cellulose	1317:1338	high-quality cellulose (Re-C)	1317:1345	Furthermore, for the solid residue of the first-step reaction, a ternary deep eutectic solvent (DES) (choline chloride/oxalic acid/1,4-butanediol, ChCl/OA/BD) has been used to separate the cellulose and lignin efficiently, and high-quality cellulose (Re-C) and lignin (Re-L) were obtained.					
37141970	1	0	link	cross-linked	185:196	arg1	beads					239:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads	174:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs)	174:280	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	3	1	from	adsorption	435:444	arg1	solutions					476:484	aqueous solutions	468:484	aqueous solutions	468:484	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	8	2	theme	composite	1121:1129	arg1	effective					1165:1173	effective	1165:1173	effective	1165:1173	This study suggests that, CMC-Ce composite with CeO2 nanoparticles is a very effective adsorbent in removing fluoride from water.					
37141970	8	2	theme	composite	1121:1129	arg1	CMC-Ce					1114:1119	CMC-Ce composite	1114:1129	CMC-Ce composite with CeO2 nanoparticles	1114:1153	This study suggests that, CMC-Ce composite with CeO2 nanoparticles is a very effective adsorbent in removing fluoride from water.					
37141970	1	3	theme	biopolymer	228:237	arg1	beads					239:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads	174:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs)	174:280	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	7	4	theme	sustainable	1044:1054	arg1	properties					1056:1065	excellent sustainable properties	1034:1065	excellent sustainable properties	1034:1065	Reusability studies showed that, the adsorbent beads have exhibited excellent sustainable properties up to 9 cycle usage.					
37141970	8	5	with	CMC-Ce	1114:1119	arg1	nanoparticles					1141:1153	CeO2 nanoparticles	1136:1153	CeO2 nanoparticles	1136:1153	This study suggests that, CMC-Ce composite with CeO2 nanoparticles is a very effective adsorbent in removing fluoride from water.					
37141970	6	6	theme	adsorption	865:874	arg1	capacity					876:883	The maximum adsorption capacity	853:883	The maximum adsorption capacity	853:883	The maximum adsorption capacity was found as 105 and 312 mg/g F- for CMC-Ce and CeO2-CMC-Ce beads, respectively.					
37141970	6	6	theme	adsorption	865:874	arg1	312 mg/g F-					906:916	312 mg/g F-	906:916	312 mg/g F-	906:916	The maximum adsorption capacity was found as 105 and 312 mg/g F- for CMC-Ce and CeO2-CMC-Ce beads, respectively.					
37141970	6	6	theme	adsorption	865:874	arg1	105					898:900	105	898:900	105	898:900	The maximum adsorption capacity was found as 105 and 312 mg/g F- for CMC-Ce and CeO2-CMC-Ce beads, respectively.					
37141970	3	7	theme	CMC	536:538	arg1	CMCCe					547:551	CMCCe	547:551	CMCCe	547:551	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	3	7	theme	CMC	536:538	arg1	beads					540:544	cerium ion cross-linked CMC beads	512:544	cerium ion cross-linked CMC beads (CMCCe)	512:552	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	0	8	theme	CeO2	87:90	arg1	nanoparticles					92:104	CeO2 nanoparticles	87:104	CeO2 nanoparticles	87:104	Removal of fluoride ions from water by cerium-carboxymethyl cellulose beads doped with CeO2 nanoparticles.					
37141970	6	9	theme	maximum	857:863	arg1	capacity					876:883	The maximum adsorption capacity	853:883	The maximum adsorption capacity	853:883	The maximum adsorption capacity was found as 105 and 312 mg/g F- for CMC-Ce and CeO2-CMC-Ce beads, respectively.					
37141970	6	9	theme	maximum	857:863	arg1	312 mg/g F-					906:916	312 mg/g F-	906:916	312 mg/g F-	906:916	The maximum adsorption capacity was found as 105 and 312 mg/g F- for CMC-Ce and CeO2-CMC-Ce beads, respectively.					
37141970	6	9	theme	maximum	857:863	arg1	105					898:900	105	898:900	105	898:900	The maximum adsorption capacity was found as 105 and 312 mg/g F- for CMC-Ce and CeO2-CMC-Ce beads, respectively.					
37141970	3	10	theme	ion	519:521	arg1	CMCCe					547:551	CMCCe	547:551	CMCCe	547:551	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	3	10	theme	ion	519:521	arg1	beads					540:544	cerium ion cross-linked CMC beads	512:544	cerium ion cross-linked CMC beads (CMCCe)	512:552	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	5	11	theme	Langmuir	801:808	arg1	isotherm					810:817	Langmuir isotherm	801:817	Langmuir isotherm	801:817	The adsorption process is well described by the Langmuir isotherm and pseudo-second-order kinetics.					
37141970	3	12	theme	ions	458:461	arg1	adsorption					435:444	The adsorption	431:444	The adsorption of fluoride ions from aqueous solutions	431:484	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	0	13	from	water	30:34	arg1	Removal					0:6	Removal	0:6	Removal of fluoride ions from water by cerium-carboxymethyl cellulose beads	0:74	Removal of fluoride ions from water by cerium-carboxymethyl cellulose beads doped with CeO2 nanoparticles.					
37141970	7	14	theme	9 cycle	1073:1079	arg1	usage					1081:1085	9 cycle usage	1073:1085	9 cycle usage	1073:1085	Reusability studies showed that, the adsorbent beads have exhibited excellent sustainable properties up to 9 cycle usage.					
37141970	3	15	theme	cross-linked	523:534	arg1	CMCCe					547:551	CMCCe	547:551	CMCCe	547:551	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	3	15	theme	cross-linked	523:534	arg1	beads					540:544	cerium ion cross-linked CMC beads	512:544	cerium ion cross-linked CMC beads (CMCCe)	512:552	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	2	16	theme	electron	365:372	arg1	microscopy					374:383	scanning electron microscopy	356:383	scanning electron microscopy	356:383	The characterization of the beads was performed by swelling experiments, scanning electron microscopy and Fourier transforms infrared spectroscopy.					
37141970	7	17	dep	usage	1081:1085	arg1	up					1067:1068	up	1067:1068	up	1067:1068	Reusability studies showed that, the adsorbent beads have exhibited excellent sustainable properties up to 9 cycle usage.					
37141970	3	18	theme	cerium	512:517	arg1	CMCCe					547:551	CMCCe	547:551	CMCCe	547:551	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	3	18	theme	cerium	512:517	arg1	beads					540:544	cerium ion cross-linked CMC beads	512:544	cerium ion cross-linked CMC beads (CMCCe)	512:552	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	3	19	from	solutions	476:484	arg1	adsorption					435:444	The adsorption	431:444	The adsorption of fluoride ions from aqueous solutions	431:484	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	3	19	from	solutions	476:484	arg1	ions					458:461	fluoride ions	449:461	fluoride ions from aqueous solutions	449:484	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	2	20	theme	scanning	356:363	arg1	microscopy					374:383	scanning electron microscopy	356:383	scanning electron microscopy	356:383	The characterization of the beads was performed by swelling experiments, scanning electron microscopy and Fourier transforms infrared spectroscopy.					
37141970	5	21	theme	adsorption	757:766	arg1	process					768:774	The adsorption process	753:774	The adsorption process	753:774	The adsorption process is well described by the Langmuir isotherm and pseudo-second-order kinetics.					
37141970	1	22	theme	CeO2	257:260	arg1	NPs					277:279	NPs	277:279	NPs	277:279	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	1	22	theme	CeO2	257:260	arg1	nanoparticles					262:274	CeO2 nanoparticles	257:274	CeO2 nanoparticles (NPs)	257:280	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	4	23	theme	contact	696:702	arg1	parameters					673:682	the parameters	669:682	the parameters such as pH	669:693	Optimized adsorption conditions were obtained by testing the parameters such as pH, contact time, adsorbent dose, and shaking rate at 25 °C.					
37141970	4	23	theme	contact	696:702	arg1	pH					692:693	pH	692:693	pH	692:693	Optimized adsorption conditions were obtained by testing the parameters such as pH, contact time, adsorbent dose, and shaking rate at 25 °C.					
37141970	4	23	theme	contact	696:702	arg1	time					704:707	contact time	696:707	contact time	696:707	Optimized adsorption conditions were obtained by testing the parameters such as pH, contact time, adsorbent dose, and shaking rate at 25 °C.					
37141970	5	24	theme	pseudo-second-order	823:841	arg1	kinetics					843:850	pseudo-second-order kinetics	823:850	pseudo-second-order kinetics	823:850	The adsorption process is well described by the Langmuir isotherm and pseudo-second-order kinetics.					
37141970	0	25	theme	ions	20:23	arg1	Removal					0:6	Removal	0:6	Removal of fluoride ions from water by cerium-carboxymethyl cellulose beads	0:74	Removal of fluoride ions from water by cerium-carboxymethyl cellulose beads doped with CeO2 nanoparticles.					
37141970	8	26	theme	CeO2	1136:1139	arg1	nanoparticles					1141:1153	CeO2 nanoparticles	1136:1153	CeO2 nanoparticles	1136:1153	This study suggests that, CMC-Ce composite with CeO2 nanoparticles is a very effective adsorbent in removing fluoride from water.					
37141970	7	27	theme	excellent	1034:1042	arg1	properties					1056:1065	excellent sustainable properties	1034:1065	excellent sustainable properties	1034:1065	Reusability studies showed that, the adsorbent beads have exhibited excellent sustainable properties up to 9 cycle usage.					
37141970	5	28	dep	isotherm	810:817	arg1	the					797:799	the	797:799	the	797:799	The adsorption process is well described by the Langmuir isotherm and pseudo-second-order kinetics.					
37141970	4	29	theme	adsorbent	710:718	arg1	parameters					673:682	the parameters	669:682	the parameters such as pH	669:693	Optimized adsorption conditions were obtained by testing the parameters such as pH, contact time, adsorbent dose, and shaking rate at 25 °C.					
37141970	4	29	theme	adsorbent	710:718	arg1	pH					692:693	pH	692:693	pH	692:693	Optimized adsorption conditions were obtained by testing the parameters such as pH, contact time, adsorbent dose, and shaking rate at 25 °C.					
37141970	4	29	theme	adsorbent	710:718	arg1	dose					720:723	adsorbent dose	710:723	adsorbent dose	710:723	Optimized adsorption conditions were obtained by testing the parameters such as pH, contact time, adsorbent dose, and shaking rate at 25 °C.					
37141970	1	30	theme	cerium	174:179	arg1	beads					239:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads	174:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs)	174:280	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	0	31	theme	fluoride	11:18	arg1	ions					20:23	fluoride ions	11:23	fluoride ions	11:23	Removal of fluoride ions from water by cerium-carboxymethyl cellulose beads doped with CeO2 nanoparticles.					
37141970	4	32	theme	shaking	730:736	arg1	rate					738:741	shaking rate	730:741	shaking rate	730:741	Optimized adsorption conditions were obtained by testing the parameters such as pH, contact time, adsorbent dose, and shaking rate at 25 °C.					
37141970	4	32	theme	shaking	730:736	arg1	parameters					673:682	the parameters	669:682	the parameters such as pH	669:693	Optimized adsorption conditions were obtained by testing the parameters such as pH, contact time, adsorbent dose, and shaking rate at 25 °C.					
37141970	4	32	theme	shaking	730:736	arg1	pH					692:693	pH	692:693	pH	692:693	Optimized adsorption conditions were obtained by testing the parameters such as pH, contact time, adsorbent dose, and shaking rate at 25 °C.					
37141970	4	33	theme	adsorption	622:631	arg1	conditions					633:642	Optimized adsorption conditions	612:642	Optimized adsorption conditions	612:642	Optimized adsorption conditions were obtained by testing the parameters such as pH, contact time, adsorbent dose, and shaking rate at 25 °C.					
37141970	1	34	theme	ion	181:183	arg1	beads					239:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads	174:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs)	174:280	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	2	35	theme	beads	311:315	arg1	characterization					287:302	The characterization	283:302	The characterization of the beads	283:315	The characterization of the beads was performed by swelling experiments, scanning electron microscopy and Fourier transforms infrared spectroscopy.					
37141970	4	36	theme	Optimized	612:620	arg1	conditions					633:642	Optimized adsorption conditions	612:642	Optimized adsorption conditions	612:642	Optimized adsorption conditions were obtained by testing the parameters such as pH, contact time, adsorbent dose, and shaking rate at 25 °C.					
37141970	2	37	dep	Fourier	389:395	arg1	transforms					397:406	transforms	397:406	transforms infrared spectroscopy	397:428	The characterization of the beads was performed by swelling experiments, scanning electron microscopy and Fourier transforms infrared spectroscopy.					
37141970	1	38	theme	cross-linked	185:196	arg1	beads					239:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads	174:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs)	174:280	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	3	39	theme	batch	598:602	arg1	system					604:609	a batch system	596:609	a batch system	596:609	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	1	40	theme	carboxymethyl	198:210	arg1	beads					239:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads	174:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs)	174:280	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	0	41	theme	cerium-carboxymethyl	39:58	arg1	beads					70:74	cerium-carboxymethyl cellulose beads	39:74	cerium-carboxymethyl cellulose beads	39:74	Removal of fluoride ions from water by cerium-carboxymethyl cellulose beads doped with CeO2 nanoparticles.					
37141970	7	42	theme	adsorbent	1003:1011	arg1	beads					1013:1017	the adsorbent beads	999:1017	the adsorbent beads	999:1017	Reusability studies showed that, the adsorbent beads have exhibited excellent sustainable properties up to 9 cycle usage.					
37141970	1	43	theme	novel	109:113	arg1	removal					148:154	A novel adsorbent for fluoride ions (F-) removal	107:154	A novel adsorbent for fluoride ions (F-) removal	107:154	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	3	44	theme	added	567:571	arg1	beads					573:577	CeO2-NPs added beads	558:577	CeO2-NPs added beads (CeO2-CMC-Ce)	558:591	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	3	44	theme	added	567:571	arg1	CeO2-CMC-Ce					580:590	CeO2-CMC-Ce	580:590	CeO2-CMC-Ce	580:590	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	7	45	theme	Reusability	966:976	arg1	studies					978:984	Reusability studies	966:984	Reusability studies	966:984	Reusability studies showed that, the adsorbent beads have exhibited excellent sustainable properties up to 9 cycle usage.					
37141970	1	46	theme	adsorbent	115:123	arg1	removal					148:154	A novel adsorbent for fluoride ions (F-) removal	107:154	A novel adsorbent for fluoride ions (F-) removal	107:154	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	3	47	theme	aqueous	468:474	arg1	solutions					476:484	aqueous solutions	468:484	aqueous solutions	468:484	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	1	48	theme	cellulose	212:220	arg1	beads					239:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads	174:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs)	174:280	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	1	49	theme	fluoride	129:136	arg1	F-					144:145	F-	144:145	F-	144:145	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	1	49	theme	fluoride	129:136	arg1	ions					138:141	fluoride ions	129:141	fluoride ions (F-)	129:146	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	3	50	theme	fluoride	449:456	arg1	ions					458:461	fluoride ions	449:461	fluoride ions from aqueous solutions	449:484	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	2	51	theme	infrared	408:415	arg1	spectroscopy					417:428	infrared spectroscopy	408:428	infrared spectroscopy	408:428	The characterization of the beads was performed by swelling experiments, scanning electron microscopy and Fourier transforms infrared spectroscopy.					
37141970	3	52	dep	added	567:571	arg1	CeO2-NPs					558:565	CeO2-NPs	558:565	CeO2-NPs	558:565	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	1	53	theme	CMC	223:225	arg1	beads					239:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads	174:243	cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs)	174:280	A novel adsorbent for fluoride ions (F-) removal was prepared from cerium ion cross-linked carboxymethyl cellulose (CMC) biopolymer beads loaded with CeO2 nanoparticles (NPs).					
37141970	0	54	theme	cellulose	60:68	arg1	beads					70:74	cerium-carboxymethyl cellulose beads	39:74	cerium-carboxymethyl cellulose beads	39:74	Removal of fluoride ions from water by cerium-carboxymethyl cellulose beads doped with CeO2 nanoparticles.					
37141970	6	55	theme	CeO2-CMC-Ce	933:943	arg1	beads					945:949	CeO2-CMC-Ce beads	933:949	CeO2-CMC-Ce beads	933:949	The maximum adsorption capacity was found as 105 and 312 mg/g F- for CMC-Ce and CeO2-CMC-Ce beads, respectively.					
37141970	2	56	theme	swelling	334:341	arg1	experiments					343:353	swelling experiments	334:353	swelling experiments	334:353	The characterization of the beads was performed by swelling experiments, scanning electron microscopy and Fourier transforms infrared spectroscopy.					
37141970	3	57	link	cross-linked	523:534	arg1	CMCCe					547:551	CMCCe	547:551	CMCCe	547:551	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
37141970	3	57	link	cross-linked	523:534	arg1	beads					540:544	cerium ion cross-linked CMC beads	512:544	cerium ion cross-linked CMC beads (CMCCe)	512:552	The adsorption of fluoride ions from aqueous solutions was carried out with both cerium ion cross-linked CMC beads (CMCCe) and CeO2-NPs added beads (CeO2-CMC-Ce) in a batch system.					
35257728	3	0	theme	packaging	361:369	arg1	requirements					371:382	the active packaging requirements	350:382	the active packaging requirements	350:382	However, traditional packaging films without antimicrobial or antioxidant activities do not satisfy the active packaging requirements.					
35257728	6	1	theme	antioxidant	587:597	arg1	activities					599:608	The antimicrobial and antioxidant activities	565:608	activities	599:608	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	8	2	theme	reduced	982:988	arg1	percentage					996:1005	significantly reduced decay percentage	968:1005	significantly reduced decay percentage	968:1005	The results confirmed that grapes treated with CS and GSE-AgNPs showed significantly reduced decay percentage, weight loss, and maintained titratable acidity at high levels compared with those of untreated fruit and fruit treated with PVP-AgNPs.					
35257728	5	3	theme	silver	475:480	arg1	nanoparticles					482:494	silver nanoparticles	475:494	silver nanoparticles (AgNPs)	475:502	In this study, silver nanoparticles (AgNPs) were synthesized from fruit waste grape seed extracts (GSE).					
35257728	5	3	theme	silver	475:480	arg1	AgNPs					497:501	AgNPs	497:501	AgNPs	497:501	In this study, silver nanoparticles (AgNPs) were synthesized from fruit waste grape seed extracts (GSE).					
35257728	8	4	theme	untreated	1093:1101	arg1	fruit					1103:1107	untreated fruit	1093:1107	untreated fruit	1093:1107	The results confirmed that grapes treated with CS and GSE-AgNPs showed significantly reduced decay percentage, weight loss, and maintained titratable acidity at high levels compared with those of untreated fruit and fruit treated with PVP-AgNPs.					
35257728	3	5	theme	traditional	259:269	arg1	films					281:285	traditional packaging films	259:285	traditional packaging films without antimicrobial or antioxidant activities	259:333	However, traditional packaging films without antimicrobial or antioxidant activities do not satisfy the active packaging requirements.					
35257728	9	6	theme	total	1198:1202	arg1	count					1209:1213	the total mold count	1194:1213	the total mold count	1194:1213	Moreover, CS and GSE-AgNPs significantly inhibited the total mold count during storage.					
35257728	6	7	theme	size	669:672	arg1	20 nm					674:678	average size 20 nm	661:678	average size 20 nm	661:678	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	6	7	theme	size	669:672	arg1	nanoparticles					624:636	GSE-silver nanoparticles	613:636	GSE-silver nanoparticles (GSE-AgNPs)	613:648	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	7	8	theme	chitosan	768:775	arg1	effect					758:763	The effect	754:763	The effect of chitosan (CS)-coated GSE-AgNPs and PVP-AgNPs on the postharvest quality of grape	754:847	The effect of chitosan (CS)-coated GSE-AgNPs and PVP-AgNPs on the postharvest quality of grape was studied during storage at 20 °C for 5 days.					
35257728	4	9	with	Films	385:389	arg1	antimicrobial					396:408	antimicrobial	396:408	antimicrobial	396:408	Films with antimicrobial and antioxidant activities are urgently required.					
35257728	4	9	with	Films	385:389	arg1	antioxidant					414:424	antioxidant	414:424	antioxidant	414:424	Films with antimicrobial and antioxidant activities are urgently required.					
35257728	9	10	theme	mold	1204:1207	arg1	count					1209:1213	the total mold count	1194:1213	the total mold count	1194:1213	Moreover, CS and GSE-AgNPs significantly inhibited the total mold count during storage.					
35257728	6	11	theme	average	661:667	arg1	20 nm					674:678	average size 20 nm	661:678	average size 20 nm	661:678	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	6	11	theme	average	661:667	arg1	nanoparticles					624:636	GSE-silver nanoparticles	613:636	GSE-silver nanoparticles (GSE-AgNPs)	613:648	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	8	12	theme	high	1058:1061	arg1	levels					1063:1068	high levels	1058:1068	high levels	1058:1068	The results confirmed that grapes treated with CS and GSE-AgNPs showed significantly reduced decay percentage, weight loss, and maintained titratable acidity at high levels compared with those of untreated fruit and fruit treated with PVP-AgNPs.					
35257728	5	13	theme	waste	532:536	arg1	extracts					549:556	fruit waste grape seed extracts	526:556	fruit waste grape seed extracts (GSE)	526:562	In this study, silver nanoparticles (AgNPs) were synthesized from fruit waste grape seed extracts (GSE).					
35257728	5	13	theme	waste	532:536	arg1	GSE					559:561	GSE	559:561	GSE	559:561	In this study, silver nanoparticles (AgNPs) were synthesized from fruit waste grape seed extracts (GSE).					
35257728	5	14	theme	seed	544:547	arg1	extracts					549:556	fruit waste grape seed extracts	526:556	fruit waste grape seed extracts (GSE)	526:562	In this study, silver nanoparticles (AgNPs) were synthesized from fruit waste grape seed extracts (GSE).					
35257728	5	14	theme	seed	544:547	arg1	GSE					559:561	GSE	559:561	GSE	559:561	In this study, silver nanoparticles (AgNPs) were synthesized from fruit waste grape seed extracts (GSE).					
35257728	8	15	theme	weight	1008:1013	arg1	loss					1015:1018	weight loss	1008:1018	weight loss	1008:1018	The results confirmed that grapes treated with CS and GSE-AgNPs showed significantly reduced decay percentage, weight loss, and maintained titratable acidity at high levels compared with those of untreated fruit and fruit treated with PVP-AgNPs.					
35257728	6	16	theme	GSE-silver	613:622	arg1	20 nm					674:678	average size 20 nm	661:678	average size 20 nm	661:678	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	6	16	theme	GSE-silver	613:622	arg1	GSE-AgNPs					639:647	GSE-AgNPs	639:647	GSE-AgNPs	639:647	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	6	16	theme	GSE-silver	613:622	arg1	nanoparticles					624:636	GSE-silver nanoparticles	613:636	GSE-silver nanoparticles (GSE-AgNPs)	613:648	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	1	17	theme	Food-borne	106:115	arg1	fungi					117:121	Food-borne fungi	106:121	Food-borne fungi	106:121	Food-borne fungi present significant hazards to food preservation and human health.					
35257728	10	18	theme	grapes	1362:1367	arg1	life					1354:1357	the shelf life	1344:1357	the shelf life of grapes	1344:1367	Our results suggest that CS coating enriched with GSE-AgNPs has the potential to preserve the quality and extend the shelf life of grapes.					
35257728	3	19	theme	packaging	271:279	arg1	films					281:285	traditional packaging films	259:285	traditional packaging films without antimicrobial or antioxidant activities	259:333	However, traditional packaging films without antimicrobial or antioxidant activities do not satisfy the active packaging requirements.					
35257728	0	20	theme	Multifunctional	0:14	arg1	chitosan/grape					16:29	Multifunctional chitosan/grape	0:29	Multifunctional chitosan/grape	0:29	Multifunctional chitosan/grape seed extract/silver nanoparticle composite for food packaging application.					
35257728	10	21	contain	has	1291:1293	arg1	coating					1259:1265	CS coating	1256:1265	CS coating enriched with GSE-AgNPs	1256:1289	Our results suggest that CS coating enriched with GSE-AgNPs has the potential to preserve the quality and extend the shelf life of grapes.					
35257728	10	21	contain	has	1291:1293	arg2	potential					1299:1307	the potential to preserve the quality and extend the shelf life of grapes	1295:1367	the potential to preserve the quality and extend the shelf life of grapes	1295:1367	Our results suggest that CS coating enriched with GSE-AgNPs has the potential to preserve the quality and extend the shelf life of grapes.					
35257728	8	22	theme	titratable	1036:1045	arg1	acidity					1047:1053	maintained titratable acidity	1025:1053	maintained titratable acidity	1025:1053	The results confirmed that grapes treated with CS and GSE-AgNPs showed significantly reduced decay percentage, weight loss, and maintained titratable acidity at high levels compared with those of untreated fruit and fruit treated with PVP-AgNPs.					
35257728	0	23	theme	extract/silver	36:49	arg1	composite					64:72	extract/silver nanoparticle composite	36:72	extract/silver nanoparticle composite	36:72	Multifunctional chitosan/grape seed extract/silver nanoparticle composite for food packaging application.					
35257728	8	24	theme	maintained	1025:1034	arg1	acidity					1047:1053	maintained titratable acidity	1025:1053	maintained titratable acidity	1025:1053	The results confirmed that grapes treated with CS and GSE-AgNPs showed significantly reduced decay percentage, weight loss, and maintained titratable acidity at high levels compared with those of untreated fruit and fruit treated with PVP-AgNPs.					
35257728	1	25	theme	significant	131:141	arg1	hazards					143:149	significant hazards	131:149	significant hazards to food preservation and human health	131:187	Food-borne fungi present significant hazards to food preservation and human health.					
35257728	5	26	theme	fruit	526:530	arg1	extracts					549:556	fruit waste grape seed extracts	526:556	fruit waste grape seed extracts (GSE)	526:562	In this study, silver nanoparticles (AgNPs) were synthesized from fruit waste grape seed extracts (GSE).					
35257728	5	26	theme	fruit	526:530	arg1	GSE					559:561	GSE	559:561	GSE	559:561	In this study, silver nanoparticles (AgNPs) were synthesized from fruit waste grape seed extracts (GSE).					
35257728	7	27	from	effect	758:763	arg1	quality					832:838	the postharvest quality	816:838	the postharvest quality of grape	816:847	The effect of chitosan (CS)-coated GSE-AgNPs and PVP-AgNPs on the postharvest quality of grape was studied during storage at 20 °C for 5 days.					
35257728	4	28	dep	antimicrobial	396:408	arg1	activities					426:435	activities	426:435	activities	426:435	Films with antimicrobial and antioxidant activities are urgently required.					
35257728	7	29	theme	postharvest	820:830	arg1	quality					832:838	the postharvest quality	816:838	the postharvest quality of grape	816:847	The effect of chitosan (CS)-coated GSE-AgNPs and PVP-AgNPs on the postharvest quality of grape was studied during storage at 20 °C for 5 days.					
35257728	5	30	theme	grape	538:542	arg1	extracts					549:556	fruit waste grape seed extracts	526:556	fruit waste grape seed extracts (GSE)	526:562	In this study, silver nanoparticles (AgNPs) were synthesized from fruit waste grape seed extracts (GSE).					
35257728	5	30	theme	grape	538:542	arg1	GSE					559:561	GSE	559:561	GSE	559:561	In this study, silver nanoparticles (AgNPs) were synthesized from fruit waste grape seed extracts (GSE).					
35257728	0	31	theme	nanoparticle	51:62	arg1	composite					64:72	extract/silver nanoparticle composite	36:72	extract/silver nanoparticle composite	36:72	Multifunctional chitosan/grape seed extract/silver nanoparticle composite for food packaging application.					
35257728	7	32	theme	PVP-AgNPs	803:811	arg1	effect					758:763	The effect	754:763	The effect of chitosan (CS)-coated GSE-AgNPs and PVP-AgNPs on the postharvest quality of grape	754:847	The effect of chitosan (CS)-coated GSE-AgNPs and PVP-AgNPs on the postharvest quality of grape was studied during storage at 20 °C for 5 days.					
35257728	0	33	theme	food	78:81	arg1	application					93:103	food packaging application	78:103	food packaging application	78:103	Multifunctional chitosan/grape seed extract/silver nanoparticle composite for food packaging application.					
35257728	6	34	theme	antimicrobial	569:581	arg1	activities					599:608	The antimicrobial and antioxidant activities	565:608	activities	599:608	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	3	35	dep	antimicrobial	295:307	arg1	activities					324:333	activities	324:333	activities	324:333	However, traditional packaging films without antimicrobial or antioxidant activities do not satisfy the active packaging requirements.					
35257728	3	36	theme	active	354:359	arg1	requirements					371:382	the active packaging requirements	350:382	the active packaging requirements	350:382	However, traditional packaging films without antimicrobial or antioxidant activities do not satisfy the active packaging requirements.					
35257728	2	37	theme	fruit	243:247	arg1	spoilage					207:214	spoilage	207:214	spoilage	207:214	Oxidation causes spoilage and the inedibility of the fruit.					
35257728	2	37	theme	fruit	243:247	arg1	inedibility					224:234	the inedibility	220:234	the inedibility of the fruit	220:247	Oxidation causes spoilage and the inedibility of the fruit.					
35257728	10	38	theme	shelf	1348:1352	arg1	life					1354:1357	the shelf life	1344:1357	the shelf life of grapes	1344:1367	Our results suggest that CS coating enriched with GSE-AgNPs has the potential to preserve the quality and extend the shelf life of grapes.					
35257728	1	39	theme	food	154:157	arg1	preservation					159:170	food preservation	154:170	food preservation	154:170	Food-borne fungi present significant hazards to food preservation and human health.					
35257728	6	40	theme	polyvinyl	695:703	arg1	PVP-AgNPs					718:726	PVP-AgNPs	718:726	PVP-AgNPs	718:726	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	6	40	theme	polyvinyl	695:703	arg1	pyrrolidone					705:715	polyvinyl pyrrolidone	695:715	polyvinyl pyrrolidone (PVP-AgNPs)	695:727	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	7	41	theme	grape	843:847	arg1	quality					832:838	the postharvest quality	816:838	the postharvest quality of grape	816:847	The effect of chitosan (CS)-coated GSE-AgNPs and PVP-AgNPs on the postharvest quality of grape was studied during storage at 20 °C for 5 days.					
35257728	7	42	theme	-coated	781:787	arg1	GSE-AgNPs					789:797	-coated GSE-AgNPs	781:797	-coated GSE-AgNPs	781:797	The effect of chitosan (CS)-coated GSE-AgNPs and PVP-AgNPs on the postharvest quality of grape was studied during storage at 20 °C for 5 days.					
35257728	7	43	dep	chitosan	768:775	arg1	GSE-AgNPs					789:797	-coated GSE-AgNPs	781:797	-coated GSE-AgNPs	781:797	The effect of chitosan (CS)-coated GSE-AgNPs and PVP-AgNPs on the postharvest quality of grape was studied during storage at 20 °C for 5 days.					
35257728	7	44	from	20 °C	879:883	arg1	storage					868:874	storage	868:874	storage at 20 °C for 5 days	868:894	The effect of chitosan (CS)-coated GSE-AgNPs and PVP-AgNPs on the postharvest quality of grape was studied during storage at 20 °C for 5 days.					
35257728	6	45	theme	AgNPs	654:658	arg1	activities					599:608	The antimicrobial and antioxidant activities	565:608	activities	599:608	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	1	46	theme	human	176:180	arg1	health					182:187	human health	176:187	human health	176:187	Food-borne fungi present significant hazards to food preservation and human health.					
35257728	10	47	theme	CS	1256:1257	arg1	coating					1259:1265	CS coating	1256:1265	CS coating enriched with GSE-AgNPs	1256:1289	Our results suggest that CS coating enriched with GSE-AgNPs has the potential to preserve the quality and extend the shelf life of grapes.					
35257728	6	48	theme	nanoparticles	624:636	arg1	activities					599:608	The antimicrobial and antioxidant activities	565:608	activities	599:608	The antimicrobial and antioxidant activities of GSE-silver nanoparticles (GSE-AgNPs) and AgNPs (average size 20 nm) stabilized by polyvinyl pyrrolidone (PVP-AgNPs) were evaluated in vitro.					
35257728	0	49	theme	packaging	83:91	arg1	application					93:103	food packaging application	78:103	food packaging application	78:103	Multifunctional chitosan/grape seed extract/silver nanoparticle composite for food packaging application.					
35257728	8	50	theme	decay	990:994	arg1	percentage					996:1005	significantly reduced decay percentage	968:1005	significantly reduced decay percentage	968:1005	The results confirmed that grapes treated with CS and GSE-AgNPs showed significantly reduced decay percentage, weight loss, and maintained titratable acidity at high levels compared with those of untreated fruit and fruit treated with PVP-AgNPs.					
36265535	1	0	theme	activated	149:157	arg1	bead					186:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead is synthesized to remove Cr(VI) and is characterized to reveal the influencing factors and reaction mechanism.					
36265535	2	1	theme	pH	513:514	arg1	m/v					544:546	m/v	544:546	m/v	544:546	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	1	theme	pH	513:514	arg1	increase					501:508	the increase	497:508	the increase of pH, and mass	497:524	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	1	theme	pH	513:514	arg1	ratio					537:541	volume ratio	530:541	volume ratio (m/v)	530:547	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	1	2	theme	carbon	159:164	arg1	bead					186:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead is synthesized to remove Cr(VI) and is characterized to reveal the influencing factors and reaction mechanism.					
36265535	5	3	theme	chemical	990:997	arg1	adsorption					1013:1022	a chemical and monolayer adsorption	988:1022	a chemical and monolayer adsorption	988:1022	Fe/CSCC adsorption for Cr(VI) is an endothermic spontaneous process, and a chemical and monolayer adsorption, which is better fitted to a pseudo-second-order kinetic.					
36265535	5	4	theme	monolayer	1003:1011	arg1	adsorption					1013:1022	a chemical and monolayer adsorption	988:1022	a chemical and monolayer adsorption	988:1022	Fe/CSCC adsorption for Cr(VI) is an endothermic spontaneous process, and a chemical and monolayer adsorption, which is better fitted to a pseudo-second-order kinetic.					
36265535	7	5	theme	Qe	1196:1197	arg1	value					1199:1203	the Qe value	1192:1203	the Qe value	1192:1203	Moreover, after five cycles of regeneration, the Qe value only drops about 3.46 mg/g.					
36265535	4	6	theme	coexisting	728:737	arg1	ions					739:742	The coexisting ions	724:742	The coexisting ions of SO42-, HPO4-, and Ca2+	724:768	The coexisting ions of SO42-, HPO4-, and Ca2+ lead to the decrease of Qe by 7.82, 5.05, and 5.50 mg/g, respectively, and the inhibition effect increases with their increasing concentrations.					
36265535	7	7	theme	regeneration	1178:1189	arg1	cycles					1168:1173	five cycles	1163:1173	five cycles of regeneration	1163:1189	Moreover, after five cycles of regeneration, the Qe value only drops about 3.46 mg/g.					
36265535	2	8	theme	adsorption	324:333	arg1	Qe					345:346	Qe	345:346	Qe	345:346	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	8	theme	adsorption	324:333	arg1	capacity					335:342	the adsorption capacity	320:342	the adsorption capacity (Qe) of Cr	320:353	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	9	theme	Cr	422:423	arg1	concentration					437:449	Cr(VI) initial concentration	422:449	Cr(VI) initial concentration (C0)	422:454	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	9	theme	Cr	422:423	arg1	loading					395:401	iron loading	390:401	iron loading	390:401	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	9	theme	Cr	422:423	arg1	C0					452:453	C0	452:453	C0	452:453	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	3	10	theme	0.1 mol	556:562	arg1	modification					570:581	0.1 mol FeCl3 modification	556:581	0.1 mol FeCl3 modification	556:581	After 0.1 mol FeCl3 modification, the removal efficiency of Cr(VI) by Fe/CSCC reaches as high as 97.25 % at pH = 3, m/v = 1.0 g/L, t = 2880 min, C0 = 25 mg/L, and T = 25 °C.					
36265535	2	11	theme	Cr	352:353	arg1	Qe					345:346	Qe	345:346	Qe	345:346	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	11	theme	Cr	352:353	arg1	capacity					335:342	the adsorption capacity	320:342	the adsorption capacity (Qe) of Cr	320:353	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	12	theme	iron	390:393	arg1	concentration					437:449	Cr(VI) initial concentration	422:449	Cr(VI) initial concentration (C0)	422:454	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	12	theme	iron	390:393	arg1	loading					395:401	iron loading	390:401	iron loading	390:401	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	12	theme	iron	390:393	arg1	time					412:415	contact time	404:415	contact time (t)	404:419	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	12	theme	iron	390:393	arg1	temperature					461:471	temperature	461:471	temperature (T)	461:475	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	6	13	theme	fitted	1086:1091	arg1	Qe					1101:1102	The fitted maximum Qe	1082:1102	The fitted maximum Qe	1082:1102	The fitted maximum Qe is 64.49 mg/g by using the Langmuir model.					
36265535	2	14	theme	contact	404:410	arg1	t					418:418	t	418:418	t	418:418	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	14	theme	contact	404:410	arg1	loading					395:401	iron loading	390:401	iron loading	390:401	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	14	theme	contact	404:410	arg1	time					412:415	contact time	404:415	contact time (t)	404:419	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	8	15	theme	SEM-EDS	1287:1293	arg1	analysis					1250:1257	Characterization analysis	1233:1257	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS	1233:1293	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS indicates that Cr(VI) is mainly adsorbed by Fe/CSCC through electrostatic attraction and complexation, which is related to the -COOH and - NH2 groups, and Fe - O groups, respectively.					
36265535	1	16	theme	Fe/CSCC	167:173	arg1	bead					186:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead is synthesized to remove Cr(VI) and is characterized to reveal the influencing factors and reaction mechanism.					
36265535	0	17	theme	modified	5:12	arg1	shell					31:35	Iron modified chitosan/coconut shell	0:35	Iron modified chitosan/coconut shell	0:35	Iron modified chitosan/coconut shell activated carbon composite beads for Cr(VI) removal from aqueous solution.					
36265535	2	18	theme	mass	521:524	arg1	m/v					544:546	m/v	544:546	m/v	544:546	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	18	theme	mass	521:524	arg1	increase					501:508	the increase	497:508	the increase of pH, and mass	497:524	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	18	theme	mass	521:524	arg1	ratio					537:541	volume ratio	530:541	volume ratio (m/v)	530:547	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	8	19	theme	Characterization	1233:1248	arg1	analysis					1250:1257	Characterization analysis	1233:1257	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS	1233:1293	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS indicates that Cr(VI) is mainly adsorbed by Fe/CSCC through electrostatic attraction and complexation, which is related to the -COOH and - NH2 groups, and Fe - O groups, respectively.					
36265535	8	20	theme	-COOH	1422:1426	arg1	groups					1438:1443	the -COOH and - NH2 groups	1418:1443	the -COOH and - NH2 groups	1418:1443	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS indicates that Cr(VI) is mainly adsorbed by Fe/CSCC through electrostatic attraction and complexation, which is related to the -COOH and - NH2 groups, and Fe - O groups, respectively.					
36265535	0	21	theme	Iron	0:3	arg1	shell					31:35	Iron modified chitosan/coconut shell	0:35	Iron modified chitosan/coconut shell	0:35	Iron modified chitosan/coconut shell activated carbon composite beads for Cr(VI) removal from aqueous solution.					
36265535	8	22	theme	XRD	1267:1269	arg1	analysis					1250:1257	Characterization analysis	1233:1257	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS	1233:1293	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS indicates that Cr(VI) is mainly adsorbed by Fe/CSCC through electrostatic attraction and complexation, which is related to the -COOH and - NH2 groups, and Fe - O groups, respectively.					
36265535	1	23	theme	composite	176:184	arg1	bead					186:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead is synthesized to remove Cr(VI) and is characterized to reveal the influencing factors and reaction mechanism.					
36265535	0	24	dep	Cr	74:75	arg1	removal					81:87	removal	81:87	Cr(VI) removal from aqueous solution	74:109	Iron modified chitosan/coconut shell activated carbon composite beads for Cr(VI) removal from aqueous solution.					
36265535	0	24	dep	Cr	74:75	arg1	VI					77:78	VI	77:78	VI	77:78	Iron modified chitosan/coconut shell activated carbon composite beads for Cr(VI) removal from aqueous solution.					
36265535	8	25	theme	Fe - O	1450:1455	arg1	groups					1457:1462	Fe - O groups	1450:1462	Fe - O groups	1450:1462	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS indicates that Cr(VI) is mainly adsorbed by Fe/CSCC through electrostatic attraction and complexation, which is related to the -COOH and - NH2 groups, and Fe - O groups, respectively.					
36265535	0	26	theme	chitosan/coconut	14:29	arg1	shell					31:35	Iron modified chitosan/coconut shell	0:35	Iron modified chitosan/coconut shell	0:35	Iron modified chitosan/coconut shell activated carbon composite beads for Cr(VI) removal from aqueous solution.					
36265535	3	27	theme	FeCl3	564:568	arg1	modification					570:581	0.1 mol FeCl3 modification	556:581	0.1 mol FeCl3 modification	556:581	After 0.1 mol FeCl3 modification, the removal efficiency of Cr(VI) by Fe/CSCC reaches as high as 97.25 % at pH = 3, m/v = 1.0 g/L, t = 2880 min, C0 = 25 mg/L, and T = 25 °C.					
36265535	4	28	theme	inhibition	849:858	arg1	effect					860:865	the inhibition effect	845:865	the inhibition effect	845:865	The coexisting ions of SO42-, HPO4-, and Ca2+ lead to the decrease of Qe by 7.82, 5.05, and 5.50 mg/g, respectively, and the inhibition effect increases with their increasing concentrations.					
36265535	0	29	theme	carbon	47:52	arg1	beads					64:68	carbon composite beads	47:68	carbon composite beads for Cr(VI) removal from aqueous solution	47:109	Iron modified chitosan/coconut shell activated carbon composite beads for Cr(VI) removal from aqueous solution.					
36265535	1	30	theme	influencing	258:268	arg1	factors					270:276	the influencing factors	254:276	the influencing factors	254:276	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead is synthesized to remove Cr(VI) and is characterized to reveal the influencing factors and reaction mechanism.					
36265535	2	31	theme	initial	429:435	arg1	concentration					437:449	Cr(VI) initial concentration	422:449	Cr(VI) initial concentration (C0)	422:454	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	31	theme	initial	429:435	arg1	loading					395:401	iron loading	390:401	iron loading	390:401	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	31	theme	initial	429:435	arg1	C0					452:453	C0	452:453	C0	452:453	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	0	32	theme	aqueous	94:100	arg1	solution					102:109	aqueous solution	94:109	aqueous solution	94:109	Iron modified chitosan/coconut shell activated carbon composite beads for Cr(VI) removal from aqueous solution.					
36265535	4	33	theme	Ca2+	765:768	arg1	ions					739:742	The coexisting ions	724:742	The coexisting ions of SO42-, HPO4-, and Ca2+	724:768	The coexisting ions of SO42-, HPO4-, and Ca2+ lead to the decrease of Qe by 7.82, 5.05, and 5.50 mg/g, respectively, and the inhibition effect increases with their increasing concentrations.					
36265535	8	34	theme	XPS	1278:1280	arg1	analysis					1250:1257	Characterization analysis	1233:1257	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS	1233:1293	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS indicates that Cr(VI) is mainly adsorbed by Fe/CSCC through electrostatic attraction and complexation, which is related to the -COOH and - NH2 groups, and Fe - O groups, respectively.					
36265535	3	35	theme	removal	588:594	arg1	efficiency					596:605	the removal efficiency	584:605	the removal efficiency of Cr	584:611	After 0.1 mol FeCl3 modification, the removal efficiency of Cr(VI) by Fe/CSCC reaches as high as 97.25 % at pH = 3, m/v = 1.0 g/L, t = 2880 min, C0 = 25 mg/L, and T = 25 °C.					
36265535	2	36	dep	concentration	437:449	arg1	VI					425:426	VI	425:426	VI	425:426	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	37	theme	loading	395:401	arg1	increase					378:385	the increase	374:385	the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T),	374:476	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	3	38	theme	Cr	610:611	arg1	efficiency					596:605	the removal efficiency	584:605	the removal efficiency of Cr	584:611	After 0.1 mol FeCl3 modification, the removal efficiency of Cr(VI) by Fe/CSCC reaches as high as 97.25 % at pH = 3, m/v = 1.0 g/L, t = 2880 min, C0 = 25 mg/L, and T = 25 °C.					
36265535	1	39	dep	Cr	216:217	arg1	VI					219:220	VI	219:220	VI	219:220	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead is synthesized to remove Cr(VI) and is characterized to reveal the influencing factors and reaction mechanism.					
36265535	2	40	dep	increases	359:367	arg1	VI					355:356	VI	355:356	VI	355:356	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	8	41	theme	and - NH2	1428:1436	arg1	groups					1438:1443	the -COOH and - NH2 groups	1418:1443	the -COOH and - NH2 groups	1418:1443	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS indicates that Cr(VI) is mainly adsorbed by Fe/CSCC through electrostatic attraction and complexation, which is related to the -COOH and - NH2 groups, and Fe - O groups, respectively.					
36265535	0	42	theme	composite	54:62	arg1	beads					64:68	carbon composite beads	47:68	carbon composite beads for Cr(VI) removal from aqueous solution	47:109	Iron modified chitosan/coconut shell activated carbon composite beads for Cr(VI) removal from aqueous solution.					
36265535	1	43	theme	reaction	282:289	arg1	mechanism					291:299	reaction mechanism	282:299	reaction mechanism	282:299	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead is synthesized to remove Cr(VI) and is characterized to reveal the influencing factors and reaction mechanism.					
36265535	4	44	theme	SO42-	747:751	arg1	ions					739:742	The coexisting ions	724:742	The coexisting ions of SO42-, HPO4-, and Ca2+	724:768	The coexisting ions of SO42-, HPO4-, and Ca2+ lead to the decrease of Qe by 7.82, 5.05, and 5.50 mg/g, respectively, and the inhibition effect increases with their increasing concentrations.					
36265535	5	45	theme	Fe/CSCC	915:921	arg1	process					975:981	an endothermic spontaneous process	948:981	an endothermic spontaneous process	948:981	Fe/CSCC adsorption for Cr(VI) is an endothermic spontaneous process, and a chemical and monolayer adsorption, which is better fitted to a pseudo-second-order kinetic.					
36265535	5	45	theme	Fe/CSCC	915:921	arg1	adsorption					923:932	Fe/CSCC adsorption	915:932	Fe/CSCC adsorption for Cr(VI)	915:943	Fe/CSCC adsorption for Cr(VI) is an endothermic spontaneous process, and a chemical and monolayer adsorption, which is better fitted to a pseudo-second-order kinetic.					
36265535	3	46	dep	reaches	628:634	arg1	VI					613:614	VI	613:614	VI	613:614	After 0.1 mol FeCl3 modification, the removal efficiency of Cr(VI) by Fe/CSCC reaches as high as 97.25 % at pH = 3, m/v = 1.0 g/L, t = 2880 min, C0 = 25 mg/L, and T = 25 °C.					
36265535	2	47	theme	volume	530:535	arg1	m/v					544:546	m/v	544:546	m/v	544:546	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	2	47	theme	volume	530:535	arg1	ratio					537:541	volume ratio	530:541	volume ratio (m/v)	530:547	Results show that the adsorption capacity (Qe) of Cr(VI) increases with the increase of iron loading, contact time (t), Cr(VI) initial concentration (C0), and temperature (T), but decreases with the increase of pH, and mass and volume ratio (m/v).					
36265535	1	48	theme	Iron	112:115	arg1	bead					186:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead is synthesized to remove Cr(VI) and is characterized to reveal the influencing factors and reaction mechanism.					
36265535	5	49	theme	endothermic	951:961	arg1	process					975:981	an endothermic spontaneous process	948:981	an endothermic spontaneous process	948:981	Fe/CSCC adsorption for Cr(VI) is an endothermic spontaneous process, and a chemical and monolayer adsorption, which is better fitted to a pseudo-second-order kinetic.					
36265535	5	49	theme	endothermic	951:961	arg1	adsorption					923:932	Fe/CSCC adsorption	915:932	Fe/CSCC adsorption for Cr(VI)	915:943	Fe/CSCC adsorption for Cr(VI) is an endothermic spontaneous process, and a chemical and monolayer adsorption, which is better fitted to a pseudo-second-order kinetic.					
36265535	1	50	theme	modified	117:124	arg1	bead					186:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead is synthesized to remove Cr(VI) and is characterized to reveal the influencing factors and reaction mechanism.					
36265535	6	51	theme	maximum	1093:1099	arg1	Qe					1101:1102	The fitted maximum Qe	1082:1102	The fitted maximum Qe	1082:1102	The fitted maximum Qe is 64.49 mg/g by using the Langmuir model.					
36265535	5	52	theme	spontaneous	963:973	arg1	process					975:981	an endothermic spontaneous process	948:981	an endothermic spontaneous process	948:981	Fe/CSCC adsorption for Cr(VI) is an endothermic spontaneous process, and a chemical and monolayer adsorption, which is better fitted to a pseudo-second-order kinetic.					
36265535	5	52	theme	spontaneous	963:973	arg1	adsorption					923:932	Fe/CSCC adsorption	915:932	Fe/CSCC adsorption for Cr(VI)	915:943	Fe/CSCC adsorption for Cr(VI) is an endothermic spontaneous process, and a chemical and monolayer adsorption, which is better fitted to a pseudo-second-order kinetic.					
36265535	4	53	theme	HPO4-	754:758	arg1	ions					739:742	The coexisting ions	724:742	The coexisting ions of SO42-, HPO4-, and Ca2+	724:768	The coexisting ions of SO42-, HPO4-, and Ca2+ lead to the decrease of Qe by 7.82, 5.05, and 5.50 mg/g, respectively, and the inhibition effect increases with their increasing concentrations.					
36265535	8	54	theme	BET	1262:1264	arg1	analysis					1250:1257	Characterization analysis	1233:1257	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS	1233:1293	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS indicates that Cr(VI) is mainly adsorbed by Fe/CSCC through electrostatic attraction and complexation, which is related to the -COOH and - NH2 groups, and Fe - O groups, respectively.					
36265535	4	55	theme	increasing	888:897	arg1	concentrations					899:912	their increasing concentrations	882:912	their increasing concentrations	882:912	The coexisting ions of SO42-, HPO4-, and Ca2+ lead to the decrease of Qe by 7.82, 5.05, and 5.50 mg/g, respectively, and the inhibition effect increases with their increasing concentrations.					
36265535	1	56	theme	chitosan/coconut	126:141	arg1	bead					186:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead is synthesized to remove Cr(VI) and is characterized to reveal the influencing factors and reaction mechanism.					
36265535	6	57	theme	Langmuir	1131:1138	arg1	model					1140:1144	the Langmuir model	1127:1144	the Langmuir model	1127:1144	The fitted maximum Qe is 64.49 mg/g by using the Langmuir model.					
36265535	5	58	dep	Cr	938:939	arg1	VI					941:942	VI	941:942	VI	941:942	Fe/CSCC adsorption for Cr(VI) is an endothermic spontaneous process, and a chemical and monolayer adsorption, which is better fitted to a pseudo-second-order kinetic.					
36265535	4	59	theme	Qe	794:795	arg1	decrease					782:789	the decrease	778:789	the decrease of Qe by 7.82, 5.05, and 5.50 mg/g, respectively	778:838	The coexisting ions of SO42-, HPO4-, and Ca2+ lead to the decrease of Qe by 7.82, 5.05, and 5.50 mg/g, respectively, and the inhibition effect increases with their increasing concentrations.					
36265535	8	60	dep	adsorbed	1327:1334	arg1	VI					1313:1314	VI	1313:1314	VI	1313:1314	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS indicates that Cr(VI) is mainly adsorbed by Fe/CSCC through electrostatic attraction and complexation, which is related to the -COOH and - NH2 groups, and Fe - O groups, respectively.					
36265535	1	61	theme	shell	143:147	arg1	bead					186:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead	112:189	Iron modified chitosan/coconut shell activated carbon (Fe/CSCC) composite bead is synthesized to remove Cr(VI) and is characterized to reveal the influencing factors and reaction mechanism.					
36265535	8	62	theme	electrostatic	1355:1367	arg1	attraction					1369:1378	electrostatic attraction	1355:1378	electrostatic attraction	1355:1378	Characterization analysis of BET, XRD, FTIR, XPS, and SEM-EDS indicates that Cr(VI) is mainly adsorbed by Fe/CSCC through electrostatic attraction and complexation, which is related to the -COOH and - NH2 groups, and Fe - O groups, respectively.					
36265535	0	63	from	solution	102:109	arg1	Cr					74:75	Cr(VI) removal from aqueous solution	74:109	Cr(VI) removal from aqueous solution	74:109	Iron modified chitosan/coconut shell activated carbon composite beads for Cr(VI) removal from aqueous solution.					
36587514	0	0	theme	gel	67:69	arg1	properties					71:80	gel properties	67:80	gel properties	67:80	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	2	1	theme	gel	432:434	arg1	performance					436:446	the gel performance	428:446	the gel performance	428:446	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	3	2	theme	starch-replacing-fat	924:943	arg1	samples					945:951	starch-replacing-fat samples	924:951	starch-replacing-fat samples	924:951	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	2	3	theme	water-holding	495:507	arg1	capacity					509:516	water-holding capacity	495:516	water-holding capacity (WHC)	495:522	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	2	3	theme	water-holding	495:507	arg1	WHC					519:521	WHC	519:521	WHC	519:521	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	3	4	theme	low-fat	1020:1026	arg1	products					1033:1040	the low-fat meat products	1016:1040	the low-fat meat products	1016:1040	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	1	5	link	crosslinked	195:205	arg1	Oil-CTS					215:221	Oil-CTS	215:221	Oil-CTS	215:221	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	1	5	link	crosslinked	195:205	arg1	starch					207:212	oil-modified crosslinked starch	182:212	oil-modified crosslinked starch (Oil-CTS) as a fat replacer	182:240	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	0	6	link	crosslinked	23:33	arg1	starch					35:40	oil-modified crosslinked starch	10:40	oil-modified crosslinked starch	10:40	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	3	7	from	5.87 g/100 g	867:878	arg1	sample					893:898	untreated sample	883:898	untreated sample	883:898	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	3	8	theme	fat	992:994	arg1	replacer					996:1003	a fat replacer	990:1003	a fat replacer	990:1003	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	2	9	theme	property	472:479	arg1	terms					451:455	terms	451:455	terms of rheological property, texture, and water-holding capacity (WHC)	451:522	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	1	10	theme	fatty	306:310	arg1	composition					317:327	fatty acid composition	306:327	fatty acid composition	306:327	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	2	11	theme	gels	568:571	arg1	mobility					546:553	the water mobility	536:553	the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure	536:657	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	2	12	theme	ordered	614:620	arg1	structure					649:657	a more ordered and denser protein network structure	607:657	a more ordered and denser protein network structure	607:657	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	1	13	theme	fat	229:231	arg1	replacer					233:240	a fat replacer	227:240	a fat replacer	227:240	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	2	14	theme	rheological	460:470	arg1	property					472:479	rheological property	460:479	rheological property	460:479	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	1	15	theme	acid	312:315	arg1	composition					317:327	fatty acid composition	306:327	fatty acid composition	306:327	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	2	16	theme	meat	563:566	arg1	gels					568:571	pork meat gels	558:571	pork meat gels	558:571	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	3	17	theme	meat	774:777	arg1	gels					779:782	pork meat gels	769:782	pork meat gels	769:782	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	3	18	theme	pork	769:772	arg1	gels					779:782	pork meat gels	769:782	pork meat gels	769:782	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	2	19	theme	pork	558:561	arg1	gels					568:571	pork meat gels	558:571	pork meat gels	558:571	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	0	20	theme	water	83:87	arg1	distribution					89:100	water distribution	83:100	water distribution	83:100	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	3	21	from	content	758:764	arg1	gels					779:782	pork meat gels	769:782	pork meat gels	769:782	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	2	22	theme	structure	649:657	arg1	formation					594:602	the formation	590:602	the formation of a more ordered and denser protein network structure	590:657	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	2	23	theme	fat	399:401	arg1	pork					389:392	pork	389:392	pork back fat	389:401	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	1	24	theme	pork	332:335	arg1	batter					342:347	pork meat batter	332:347	pork meat batter	332:347	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	2	25	theme	water	540:544	arg1	mobility					546:553	the water mobility	536:553	the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure	536:657	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	2	26	theme	network	641:647	arg1	structure					649:657	a more ordered and denser protein network structure	607:657	a more ordered and denser protein network structure	607:657	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	0	27	theme	crosslinked	23:33	arg1	starch					35:40	oil-modified crosslinked starch	10:40	oil-modified crosslinked starch	10:40	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	3	28	theme	untreated	883:891	arg1	sample					893:898	untreated sample	883:898	untreated sample	883:898	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	0	29	theme	oil-modified	10:21	arg1	starch					35:40	oil-modified crosslinked starch	10:40	oil-modified crosslinked starch	10:40	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	1	30	theme	gel	249:251	arg1	properties					253:262	the gel properties	245:262	the gel properties	245:262	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	1	31	theme	meat	337:340	arg1	batter					342:347	pork meat batter	332:347	pork meat batter	332:347	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	2	32	theme	texture	482:488	arg1	terms					451:455	terms	451:455	terms of rheological property, texture, and water-holding capacity (WHC)	451:522	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	1	33	theme	batter	342:347	arg1	composition					317:327	fatty acid composition	306:327	fatty acid composition	306:327	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	1	33	theme	batter	342:347	arg1	microstructures					285:299	microstructures	285:299	microstructures	285:299	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	1	33	theme	batter	342:347	arg1	properties					253:262	the gel properties	245:262	the gel properties	245:262	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	1	33	theme	batter	342:347	arg1	distribution					271:282	water distribution	265:282	water distribution	265:282	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	1	34	theme	oil-modified	182:193	arg1	Oil-CTS					215:221	Oil-CTS	215:221	Oil-CTS	215:221	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	1	34	theme	oil-modified	182:193	arg1	starch					207:212	oil-modified crosslinked starch	182:212	oil-modified crosslinked starch (Oil-CTS) as a fat replacer	182:240	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	0	35	theme	starch	35:40	arg1	Effect					0:5	Effect	0:5	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.	0:142	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	0	36	from	replacer	55:62	arg1	microstructures					107:121	microstructures	107:121	microstructures of pork meat batter	107:141	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	0	36	from	replacer	55:62	arg1	properties					71:80	gel properties	67:80	gel properties	67:80	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	0	36	from	replacer	55:62	arg1	distribution					89:100	water distribution	83:100	water distribution	83:100	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	2	37	theme	pork	389:392	arg1	replacement					374:384	the replacement	370:384	the replacement of pork back fat by Oil-CTS	370:412	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	3	38	theme	saturated	815:823	arg1	SFAs					838:841	SFAs	838:841	SFAs	838:841	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	3	38	theme	saturated	815:823	arg1	acids					831:835	the saturated fatty acids	811:835	the saturated fatty acids (SFAs) content	811:850	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	1	39	theme	crosslinked	195:205	arg1	Oil-CTS					215:221	Oil-CTS	215:221	Oil-CTS	215:221	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	1	39	theme	crosslinked	195:205	arg1	starch					207:212	oil-modified crosslinked starch	182:212	oil-modified crosslinked starch (Oil-CTS) as a fat replacer	182:240	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	0	40	theme	new	47:49	arg1	replacer					55:62	a new fat replacer	45:62	a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter	45:141	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	1	41	theme	water	265:269	arg1	distribution					271:282	water distribution	265:282	water distribution	265:282	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	0	42	theme	meat	131:134	arg1	batter					136:141	pork meat batter	126:141	pork meat batter	126:141	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	3	43	theme	fatty	825:829	arg1	SFAs					838:841	SFAs	838:841	SFAs	838:841	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	3	43	theme	fatty	825:829	arg1	acids					831:835	the saturated fatty acids	811:835	the saturated fatty acids (SFAs) content	811:850	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	3	44	dep	Oil-CTS	965:971	arg1	used					982:985	used	982:985	could be used as a fat replacer to prepare the low-fat meat products	973:1040	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	0	45	theme	pork	126:129	arg1	batter					136:141	pork meat batter	126:141	pork meat batter	126:141	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	1	46	from	effects	171:177	arg1	composition					317:327	fatty acid composition	306:327	fatty acid composition	306:327	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	1	46	from	effects	171:177	arg1	microstructures					285:299	microstructures	285:299	microstructures	285:299	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	1	46	from	effects	171:177	arg1	properties					253:262	the gel properties	245:262	the gel properties	245:262	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	1	46	from	effects	171:177	arg1	distribution					271:282	water distribution	265:282	water distribution	265:282	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	2	47	theme	capacity	509:516	arg1	terms					451:455	terms	451:455	terms of rheological property, texture, and water-holding capacity (WHC)	451:522	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	3	48	dep	or	721:722	arg1	%					740:740	25-100 %	733:740	25-100 %	733:740	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	1	49	theme	starch	207:212	arg1	effects					171:177	the effects	167:177	the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter	167:347	This work investigated the effects of oil-modified crosslinked starch (Oil-CTS) as a fat replacer on the gel properties, water distribution, microstructures, and fatty acid composition of pork meat batter.					
36587514	0	50	theme	batter	136:141	arg1	microstructures					107:121	microstructures	107:121	microstructures of pork meat batter	107:141	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	0	50	theme	batter	136:141	arg1	properties					71:80	gel properties	67:80	gel properties	67:80	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	0	50	theme	batter	136:141	arg1	distribution					89:100	water distribution	83:100	water distribution	83:100	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	2	51	theme	protein	633:639	arg1	structure					649:657	a more ordered and denser protein network structure	607:657	a more ordered and denser protein network structure	607:657	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	3	52	theme	meat	1028:1031	arg1	products					1033:1040	the low-fat meat products	1016:1040	the low-fat meat products	1016:1040	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	3	53	theme	total	748:752	arg1	content					758:764	the total fat content	744:764	the total fat content in pork meat gels	744:782	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	2	54	theme	denser	626:631	arg1	structure					649:657	a more ordered and denser protein network structure	607:657	a more ordered and denser protein network structure	607:657	Results showed that the replacement of pork back fat by Oil-CTS could improve the gel performance in terms of rheological property, texture, and water-holding capacity (WHC), and reduce the water mobility of pork meat gels, which caused by the formation of a more ordered and denser protein network structure.					
36587514	0	55	theme	fat	51:53	arg1	replacer					55:62	a new fat replacer	45:62	a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter	45:141	Effect of oil-modified crosslinked starch as a new fat replacer on gel properties, water distribution, and microstructures of pork meat batter.					
36587514	3	56	theme	acids	831:835	arg1	content					844:850	the saturated fatty acids (SFAs) content	811:850	the saturated fatty acids (SFAs) content	811:850	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
36587514	3	57	theme	fat	754:756	arg1	content					758:764	the total fat content	744:764	the total fat content in pork meat gels	744:782	Additionally, when the fat was replaced by Oil-CTS partially or totally (25-100 %), the total fat content in pork meat gels decreased by 16.5-82 % and the saturated fatty acids (SFAs) content decreased from 5.87 g/100 g in untreated sample to 1.17-4.88 g/100 g in starch-replacing-fat samples, indicating Oil-CTS could be used as a fat replacer to prepare the low-fat meat products.					
35609584	1	0	theme	mouse	166:170	arg1	cells					179:183	mouse cancer cells	166:183	mouse cancer cells	166:183	Matrigel is a polymeric extracellular matrix material produced by mouse cancer cells.					
35609584	0	1	theme	improved	77:84	arg1	permeability					86:97	improved permeability	77:97	improved permeability	77:97	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.					
35609584	8	2	theme	restructuring	1159:1171	arg1	Matrigel					1173:1180	restructuring Matrigel	1159:1180	restructuring Matrigel in this manner	1159:1195	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	1	3	theme	cancer	172:177	arg1	cells					179:183	mouse cancer cells	166:183	mouse cancer cells	166:183	Matrigel is a polymeric extracellular matrix material produced by mouse cancer cells.					
35609584	5	4	dep	strategy	563:570	arg1	improve					575:581	improve	575:581	to improve transport through a hydrogel without modifying the chemistry or composition of the gel	572:668	A strategy to improve transport through a hydrogel without modifying the chemistry or composition of the gel is to physically restructure the material into microscopic microgels and then pack them together to form a porous material.					
35609584	3	5	theme	widespread	360:369	arg1	use					371:373	widespread use	360:373	widespread use	360:373	Despite widespread use, transport of molecules, cells, and colloidal particles through Matrigel can be limited.					
35609584	6	6	theme	granular	883:890	arg1	hydrogels					892:900	granular hydrogels	883:900	granular hydrogels composed of Matrigel	883:921	These 'granular' hydrogels have been created using a variety of synthetic hydrogels, but granular hydrogels composed of Matrigel have not yet been reported.					
35609584	6	7	theme	granular	801:808	arg1	hydrogels					811:819	These 'granular' hydrogels	794:819	These 'granular' hydrogels	794:819	These 'granular' hydrogels have been created using a variety of synthetic hydrogels, but granular hydrogels composed of Matrigel have not yet been reported.					
35609584	8	8	with	organoids	1366:1374	arg1	HGOs					1416:1419	HGOs	1416:1419	HGOs	1416:1419	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	8	9	theme	organoids	1366:1374	arg1	culture					1341:1347	culture	1341:1347	culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs	1341:1419	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	5	10	theme	gel	666:668	arg1	chemistry					634:642	chemistry	634:642	chemistry	634:642	A strategy to improve transport through a hydrogel without modifying the chemistry or composition of the gel is to physically restructure the material into microscopic microgels and then pack them together to form a porous material.					
35609584	5	10	theme	gel	666:668	arg1	composition					647:657	composition	647:657	composition	647:657	A strategy to improve transport through a hydrogel without modifying the chemistry or composition of the gel is to physically restructure the material into microscopic microgels and then pack them together to form a porous material.					
35609584	7	11	theme	Matrigel	1093:1100	arg1	microgels					1102:1110	packed Matrigel microgels	1086:1110	packed Matrigel microgels	1086:1110	Here we present a drop-based microfluidics approach for structuring Matrigel into a three-dimensional, mesoporous material composed of packed Matrigel microgels, which we call granular Matrigel.					
35609584	8	12	theme	human	1352:1356	arg1	organoids					1366:1374	human gastric organoids	1352:1374	human gastric organoids (HGOs)	1352:1381	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	8	12	theme	human	1352:1356	arg1	HGOs					1377:1380	HGOs	1377:1380	HGOs	1377:1380	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	8	13	theme	dendritic	1253:1261	arg1	DCs					1270:1272	DCs	1270:1272	DCs	1270:1272	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	8	13	theme	dendritic	1253:1261	arg1	cells					1263:1267	human dendritic cells	1247:1267	human dendritic cells (DCs)	1247:1273	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	0	14	theme	extracellular	43:55	arg1	material					64:71	a trusted extracellular matrix material	33:71	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.	0:98	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.					
35609584	6	15	theme	hydrogels	868:876	arg1	variety					847:853	a variety	845:853	a variety of synthetic hydrogels	845:876	These 'granular' hydrogels have been created using a variety of synthetic hydrogels, but granular hydrogels composed of Matrigel have not yet been reported.					
35609584	6	15	theme	hydrogels	868:876	arg1	hydrogels					868:876	synthetic hydrogels	858:876	synthetic hydrogels	858:876	These 'granular' hydrogels have been created using a variety of synthetic hydrogels, but granular hydrogels composed of Matrigel have not yet been reported.					
35609584	5	16	dep	chemistry	634:642	arg1	the					630:632	the	630:632	the	630:632	A strategy to improve transport through a hydrogel without modifying the chemistry or composition of the gel is to physically restructure the material into microscopic microgels and then pack them together to form a porous material.					
35609584	0	17	theme	Granular	0:7	arg1	Matrigel					9:16	Granular Matrigel	0:16	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.	0:98	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.					
35609584	8	18	theme	particles	1233:1241	arg1	transport					1210:1218	the transport	1206:1218	the transport of colloidal particles and human dendritic cells (DCs) through the gel	1206:1289	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	6	19	theme	synthetic	858:866	arg1	hydrogels					868:876	synthetic hydrogels	858:876	synthetic hydrogels	858:876	These 'granular' hydrogels have been created using a variety of synthetic hydrogels, but granular hydrogels composed of Matrigel have not yet been reported.					
35609584	2	20	theme	tissue	303:308	arg1	applications					318:329	two- and three-dimensional cell and tissue culture applications	267:329	applications	318:329	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	3	21	theme	colloidal	411:419	arg1	particles					421:429	colloidal particles	411:429	colloidal particles	411:429	Despite widespread use, transport of molecules, cells, and colloidal particles through Matrigel can be limited.					
35609584	3	22	theme	molecules	389:397	arg1	transport					376:384	transport	376:384	transport of molecules, cells, and colloidal particles through Matrigel	376:446	Despite widespread use, transport of molecules, cells, and colloidal particles through Matrigel can be limited.					
35609584	4	23	dep	cell	491:494	arg1	viability					504:512	viability	504:512	viability	504:512	These limitations restrict cell growth, viability, and function and limit Matrigel applications.					
35609584	4	23	dep	cell	491:494	arg1	function					519:526	function	519:526	function	519:526	These limitations restrict cell growth, viability, and function and limit Matrigel applications.					
35609584	4	23	dep	cell	491:494	arg1	growth					496:501	growth	496:501	growth	496:501	These limitations restrict cell growth, viability, and function and limit Matrigel applications.					
35609584	7	24	theme	packed	1086:1091	arg1	microgels					1102:1110	packed Matrigel microgels	1086:1110	packed Matrigel microgels	1086:1110	Here we present a drop-based microfluidics approach for structuring Matrigel into a three-dimensional, mesoporous material composed of packed Matrigel microgels, which we call granular Matrigel.					
35609584	8	25	theme	gastric	1358:1364	arg1	organoids					1366:1374	human gastric organoids	1352:1374	human gastric organoids (HGOs)	1352:1381	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	8	25	theme	gastric	1358:1364	arg1	HGOs					1377:1380	HGOs	1377:1380	HGOs	1377:1380	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	3	26	theme	particles	421:429	arg1	transport					376:384	transport	376:384	transport of molecules, cells, and colloidal particles through Matrigel	376:446	Despite widespread use, transport of molecules, cells, and colloidal particles through Matrigel can be limited.					
35609584	4	27	theme	Matrigel	538:545	arg1	applications					547:558	Matrigel applications	538:558	Matrigel applications	538:558	These limitations restrict cell growth, viability, and function and limit Matrigel applications.					
35609584	2	28	theme	cell	294:297	arg1	cell					294:297	two- and three-dimensional cell and tissue culture applications	267:329	cell	294:297	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	2	28	theme	cell	294:297	arg1	variety					256:262	a wide variety	249:262	a wide variety of two- and three-dimensional cell and tissue culture applications including organoids	249:349	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	2	28	theme	cell	294:297	arg1	organoids					341:349	organoids	341:349	organoids	341:349	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	2	28	theme	cell	294:297	arg1	applications					318:329	two- and three-dimensional cell and tissue culture applications	267:329	applications	318:329	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	8	29	theme	mechanical	1318:1327	arg1	support					1329:1335	sufficient mechanical support	1307:1335	sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs	1307:1419	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	8	30	theme	cells	1263:1267	arg1	transport					1210:1218	the transport	1206:1218	the transport of colloidal particles and human dendritic cells (DCs) through the gel	1206:1289	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	0	31	theme	trusted	35:41	arg1	material					64:71	a trusted extracellular matrix material	33:71	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.	0:98	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.					
35609584	8	32	theme	human	1247:1251	arg1	DCs					1270:1272	DCs	1270:1272	DCs	1270:1272	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	8	32	theme	human	1247:1251	arg1	cells					1263:1267	human dendritic cells	1247:1267	human dendritic cells (DCs)	1247:1273	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	5	33	theme	porous	777:782	arg1	material					784:791	a porous material	775:791	a porous material	775:791	A strategy to improve transport through a hydrogel without modifying the chemistry or composition of the gel is to physically restructure the material into microscopic microgels and then pack them together to form a porous material.					
35609584	5	34	theme	microscopic	717:727	arg1	microgels					729:737	microscopic microgels	717:737	microscopic microgels	717:737	A strategy to improve transport through a hydrogel without modifying the chemistry or composition of the gel is to physically restructure the material into microscopic microgels and then pack them together to form a porous material.					
35609584	7	35	dep	three-dimensional	1035:1051	arg1	mesoporous					1054:1063	mesoporous	1054:1063	mesoporous	1054:1063	Here we present a drop-based microfluidics approach for structuring Matrigel into a three-dimensional, mesoporous material composed of packed Matrigel microgels, which we call granular Matrigel.					
35609584	0	36	theme	matrix	57:62	arg1	material					64:71	a trusted extracellular matrix material	33:71	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.	0:98	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.					
35609584	8	37	with	co-culture	1387:1396	arg1	HGOs					1416:1419	HGOs	1416:1419	HGOs	1416:1419	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	1	38	theme	polymeric	114:122	arg1	Matrigel					100:107	Matrigel	100:107	Matrigel	100:107	Matrigel is a polymeric extracellular matrix material produced by mouse cancer cells.					
35609584	1	38	theme	polymeric	114:122	arg1	material					145:152	a polymeric extracellular matrix material	112:152	a polymeric extracellular matrix material produced by mouse cancer cells	112:183	Matrigel is a polymeric extracellular matrix material produced by mouse cancer cells.					
35609584	3	39	theme	cells	400:404	arg1	transport					376:384	transport	376:384	transport of molecules, cells, and colloidal particles through Matrigel	376:446	Despite widespread use, transport of molecules, cells, and colloidal particles through Matrigel can be limited.					
35609584	7	40	theme	three-dimensional	1035:1051	arg1	material					1065:1072	a three-dimensional, mesoporous material	1033:1072	a three-dimensional, mesoporous material composed of packed Matrigel microgels, which we call granular Matrigel	1033:1143	Here we present a drop-based microfluidics approach for structuring Matrigel into a three-dimensional, mesoporous material composed of packed Matrigel microgels, which we call granular Matrigel.					
35609584	8	41	from	Matrigel	1173:1180	arg1	manner					1190:1195	this manner	1185:1195	this manner	1185:1195	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	8	42	theme	co-culture	1387:1396	arg1	culture					1341:1347	culture	1341:1347	culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs	1341:1419	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	7	43	theme	microfluidics	980:992	arg1	approach					994:1001	a drop-based microfluidics approach	967:1001	a drop-based microfluidics approach for structuring Matrigel into a three-dimensional, mesoporous material composed of packed Matrigel microgels, which we call granular Matrigel	967:1143	Here we present a drop-based microfluidics approach for structuring Matrigel into a three-dimensional, mesoporous material composed of packed Matrigel microgels, which we call granular Matrigel.					
35609584	1	44	theme	extracellular	124:136	arg1	Matrigel					100:107	Matrigel	100:107	Matrigel	100:107	Matrigel is a polymeric extracellular matrix material produced by mouse cancer cells.					
35609584	1	44	theme	extracellular	124:136	arg1	material					145:152	a polymeric extracellular matrix material	112:152	a polymeric extracellular matrix material produced by mouse cancer cells	112:183	Matrigel is a polymeric extracellular matrix material produced by mouse cancer cells.					
35609584	8	45	theme	DCs	1407:1409	arg1	co-culture					1387:1396	co-culture	1387:1396	co-culture of human DCs with HGOs	1387:1419	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	8	45	theme	DCs	1407:1409	arg1	organoids					1366:1374	human gastric organoids	1352:1374	human gastric organoids (HGOs)	1352:1381	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	8	45	theme	DCs	1407:1409	arg1	HGOs					1377:1380	HGOs	1377:1380	HGOs	1377:1380	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	2	46	theme	wide	251:254	arg1	cell					294:297	two- and three-dimensional cell and tissue culture applications	267:329	cell	294:297	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	2	46	theme	wide	251:254	arg1	variety					256:262	a wide variety	249:262	a wide variety of two- and three-dimensional cell and tissue culture applications including organoids	249:349	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	2	46	theme	wide	251:254	arg1	organoids					341:349	organoids	341:349	organoids	341:349	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	2	46	theme	wide	251:254	arg1	applications					318:329	two- and three-dimensional cell and tissue culture applications	267:329	applications	318:329	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	0	47	dep	restructuring	19:31	arg1	material					64:71	a trusted extracellular matrix material	33:71	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.	0:98	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.					
35609584	1	48	theme	matrix	138:143	arg1	Matrigel					100:107	Matrigel	100:107	Matrigel	100:107	Matrigel is a polymeric extracellular matrix material produced by mouse cancer cells.					
35609584	1	48	theme	matrix	138:143	arg1	material					145:152	a polymeric extracellular matrix material	112:152	a polymeric extracellular matrix material produced by mouse cancer cells	112:183	Matrigel is a polymeric extracellular matrix material produced by mouse cancer cells.					
35609584	8	49	theme	human	1401:1405	arg1	DCs					1407:1409	human DCs	1401:1409	human DCs	1401:1409	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	2	50	theme	culture	310:316	arg1	applications					318:329	two- and three-dimensional cell and tissue culture applications	267:329	applications	318:329	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	8	51	theme	colloidal	1223:1231	arg1	particles					1233:1241	colloidal particles	1223:1241	colloidal particles	1223:1241	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	2	52	theme	applications	318:329	arg1	cell					294:297	two- and three-dimensional cell and tissue culture applications	267:329	cell	294:297	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	2	52	theme	applications	318:329	arg1	variety					256:262	a wide variety	249:262	a wide variety of two- and three-dimensional cell and tissue culture applications including organoids	249:349	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	2	52	theme	applications	318:329	arg1	organoids					341:349	organoids	341:349	organoids	341:349	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	2	52	theme	applications	318:329	arg1	applications					318:329	two- and three-dimensional cell and tissue culture applications	267:329	applications	318:329	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	2	53	theme	past	195:198	arg1	decades					205:211	the past four decades	191:211	the past four decades	191:211	Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids.					
35609584	0	54	dep	Matrigel	9:16	arg1	restructuring					19:31	restructuring	19:31	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.	0:98	Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.					
35609584	7	55	theme	drop-based	969:978	arg1	approach					994:1001	a drop-based microfluidics approach	967:1001	a drop-based microfluidics approach for structuring Matrigel into a three-dimensional, mesoporous material composed of packed Matrigel microgels, which we call granular Matrigel	967:1143	Here we present a drop-based microfluidics approach for structuring Matrigel into a three-dimensional, mesoporous material composed of packed Matrigel microgels, which we call granular Matrigel.					
35609584	8	56	theme	sufficient	1307:1316	arg1	support					1329:1335	sufficient mechanical support	1307:1335	sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs	1307:1419	We show that restructuring Matrigel in this manner enhances the transport of colloidal particles and human dendritic cells (DCs) through the gel while providing sufficient mechanical support for culture of human gastric organoids (HGOs) and co-culture of human DCs with HGOs.					
35609584	7	57	theme	granular	1127:1134	arg1	Matrigel					1136:1143	granular Matrigel	1127:1143	granular Matrigel	1127:1143	Here we present a drop-based microfluidics approach for structuring Matrigel into a three-dimensional, mesoporous material composed of packed Matrigel microgels, which we call granular Matrigel.					
36183605	0	0	theme	exploration	84:94	arg1	identification					13:26	identification	13:26	identification	13:26	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	0	0	theme	exploration	84:94	arg1	Analysis					0:7	Analysis	0:7	Analysis	0:7	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	0	1	from	identification	13:26	arg1	Wurile					73:78	Wurile	73:78	Wurile	73:78	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	2	2	theme	genes	376:380	arg1	expression					345:354	the expression	341:354	the expression of pro-inflammatory genes in LPS stimulated microglia	341:408	Fraction 4-6 (F4-6) isolated from EW via serial fractionation inhibits the expression of pro-inflammatory genes in LPS stimulated microglia.					
36183605	5	3	theme	Ala	677:679	arg1	F4-6					647:650	F4-6	647:650	F4-6	647:650	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	3	theme	Ala	677:679	arg1	mixture					666:672	mixture	666:672	mixture of Ala and Deh (Mix)	666:693	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	3	theme	Ala	677:679	arg1	Deh					658:660	Deh	658:660	Deh	658:660	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	3	theme	Ala	677:679	arg1	Ala					653:655	Ala	653:655	Ala	653:655	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	4	theme	pro-inflammatory	734:749	arg1	Cox2					773:776	Cox2	773:776	Cox2	773:776	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	4	theme	pro-inflammatory	734:749	arg1	Ccl2					767:770	Ccl2	767:770	Ccl2	767:770	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	4	theme	pro-inflammatory	734:749	arg1	genes					751:755	several pro-inflammatory genes	726:755	several pro-inflammatory genes including Ccl2, Cox2 and Il6	726:784	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	4	theme	pro-inflammatory	734:749	arg1	Il6					782:784	Il6	782:784	Il6	782:784	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	7	5	theme	neurite	1083:1089	arg1	outgrowth					1091:1099	neurite outgrowth	1083:1099	neurite outgrowth	1083:1099	Moreover, the conditioned culture media obtained from F4-6 treated microglia significantly enhanced proliferation of N2a cells, and promoted neurite outgrowth possibly through upregulation of Nefh and Dlg4.					
36183605	2	6	theme	pro-inflammatory	359:374	arg1	genes					376:380	pro-inflammatory genes	359:380	pro-inflammatory genes	359:380	Fraction 4-6 (F4-6) isolated from EW via serial fractionation inhibits the expression of pro-inflammatory genes in LPS stimulated microglia.					
36183605	1	7	dep	microglia	246:254	arg1	polarization					256:267	polarization	256:267	microglia polarization	246:267	Ethnomedicine Eerdun Wurile (EW) can significantly promote poststroke neuro-recovery through modulation of microglia polarization.					
36183605	0	8	from	Analysis	0:7	arg1	Wurile					73:78	Wurile	73:78	Wurile	73:78	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	4	9	theme	F4-6	597:600	arg1	components					583:592	the active anti-inflammatory components	554:592	the active anti-inflammatory components of F4-6	554:600	Herein, we identified alantolactone (Ala) and dehydrodiisoeugenol (Deh) as the active anti-inflammatory components of F4-6 by UPLC-qTof MS analysis.					
36183605	4	9	theme	F4-6	597:600	arg1	alantolactone					501:513	alantolactone	501:513	alantolactone (Ala)	501:519	Herein, we identified alantolactone (Ala) and dehydrodiisoeugenol (Deh) as the active anti-inflammatory components of F4-6 by UPLC-qTof MS analysis.					
36183605	4	9	theme	F4-6	597:600	arg1	dehydrodiisoeugenol					525:543	dehydrodiisoeugenol	525:543	dehydrodiisoeugenol (Deh)	525:549	Herein, we identified alantolactone (Ala) and dehydrodiisoeugenol (Deh) as the active anti-inflammatory components of F4-6 by UPLC-qTof MS analysis.					
36183605	9	10	theme	herbal	1492:1497	arg1	composition					1499:1509	the herbal composition	1488:1509	the herbal composition for neuroprotection	1488:1529	Collectively, our data identified and quantified the key chemicals of EW and provide insights into the optimization of the herbal composition for neuroprotection.					
36183605	0	11	from	molecules	53:61	arg1	Wurile					73:78	Wurile	73:78	Wurile	73:78	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	5	12	theme	LPS-treated	789:799	arg1	microglia					801:809	LPS-treated microglia	789:809	LPS-treated microglia	789:809	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	2	13	from	expression	345:354	arg1	microglia					400:408	LPS stimulated microglia	385:408	LPS stimulated microglia	385:408	Fraction 4-6 (F4-6) isolated from EW via serial fractionation inhibits the expression of pro-inflammatory genes in LPS stimulated microglia.					
36183605	3	14	theme	F4-6	448:451	arg1	molecules					435:443	the key active molecules	420:443	the key active molecules of F4-6	420:451	However, the key active molecules of F4-6 have not been identified.					
36183605	0	15	theme	mechanism	105:113	arg1	exploration					84:94	exploration	84:94	exploration of their mechanism of action in microglia	84:136	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	0	15	theme	mechanism	105:113	arg1	molecules					53:61	key anti-inflammatory molecules	31:61	key anti-inflammatory molecules in Eerdun Wurile	31:78	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	3	16	theme	key	424:426	arg1	molecules					435:443	the key active molecules	420:443	the key active molecules of F4-6	420:451	However, the key active molecules of F4-6 have not been identified.					
36183605	8	17	theme	transcription	1309:1321	arg1	suppression					1294:1304	the suppression	1290:1304	the suppression of transcription of pro-inflammatory genes initiated by NF-κB	1290:1366	Mechanistically, F4-6 strongly downregulated the expression of NF-κB p65, while also inhibiting the nuclear translocation of p65, leading to the suppression of transcription of pro-inflammatory genes initiated by NF-κB.					
36183605	5	18	theme	similar	816:822	arg1	pattern					824:830	a similar pattern	814:830	a similar pattern	814:830	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	19	theme	several	726:732	arg1	Cox2					773:776	Cox2	773:776	Cox2	773:776	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	19	theme	several	726:732	arg1	Ccl2					767:770	Ccl2	767:770	Ccl2	767:770	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	19	theme	several	726:732	arg1	genes					751:755	several pro-inflammatory genes	726:755	several pro-inflammatory genes including Ccl2, Cox2 and Il6	726:784	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	19	theme	several	726:732	arg1	Il6					782:784	Il6	782:784	Il6	782:784	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	8	20	theme	genes	1343:1347	arg1	transcription					1309:1321	transcription	1309:1321	transcription of pro-inflammatory genes initiated by NF-κB	1309:1366	Mechanistically, F4-6 strongly downregulated the expression of NF-κB p65, while also inhibiting the nuclear translocation of p65, leading to the suppression of transcription of pro-inflammatory genes initiated by NF-κB.					
36183605	6	21	theme	same	840:843	arg1	time					845:848	the same time	836:848	the same time	836:848	At the same time upregulate the expression of anti-inflammatory genes including Hmox1, Tgfβ, Igf1 and Creb1.					
36183605	9	22	theme	key	1422:1424	arg1	chemicals					1426:1434	the key chemicals	1418:1434	the key chemicals of EW	1418:1440	Collectively, our data identified and quantified the key chemicals of EW and provide insights into the optimization of the herbal composition for neuroprotection.					
36183605	7	23	theme	Dlg4	1143:1146	arg1	upregulation					1118:1129	upregulation	1118:1129	upregulation of Nefh and Dlg4	1118:1146	Moreover, the conditioned culture media obtained from F4-6 treated microglia significantly enhanced proliferation of N2a cells, and promoted neurite outgrowth possibly through upregulation of Nefh and Dlg4.					
36183605	8	24	theme	NF-κB	1212:1216	arg1	p65					1218:1220	NF-κB p65	1212:1220	NF-κB p65	1212:1220	Mechanistically, F4-6 strongly downregulated the expression of NF-κB p65, while also inhibiting the nuclear translocation of p65, leading to the suppression of transcription of pro-inflammatory genes initiated by NF-κB.					
36183605	2	25	theme	serial	311:316	arg1	fractionation					318:330	serial fractionation	311:330	serial fractionation	311:330	Fraction 4-6 (F4-6) isolated from EW via serial fractionation inhibits the expression of pro-inflammatory genes in LPS stimulated microglia.					
36183605	2	26	theme	stimulated	389:398	arg1	microglia					400:408	LPS stimulated microglia	385:408	LPS stimulated microglia	385:408	Fraction 4-6 (F4-6) isolated from EW via serial fractionation inhibits the expression of pro-inflammatory genes in LPS stimulated microglia.					
36183605	9	27	theme	EW	1439:1440	arg1	chemicals					1426:1434	the key chemicals	1418:1434	the key chemicals of EW	1418:1440	Collectively, our data identified and quantified the key chemicals of EW and provide insights into the optimization of the herbal composition for neuroprotection.					
36183605	7	28	theme	Nefh	1134:1137	arg1	upregulation					1118:1129	upregulation	1118:1129	upregulation of Nefh and Dlg4	1118:1146	Moreover, the conditioned culture media obtained from F4-6 treated microglia significantly enhanced proliferation of N2a cells, and promoted neurite outgrowth possibly through upregulation of Nefh and Dlg4.					
36183605	4	29	theme	active	558:563	arg1	components					583:592	the active anti-inflammatory components	554:592	the active anti-inflammatory components of F4-6	554:600	Herein, we identified alantolactone (Ala) and dehydrodiisoeugenol (Deh) as the active anti-inflammatory components of F4-6 by UPLC-qTof MS analysis.					
36183605	4	29	theme	active	558:563	arg1	alantolactone					501:513	alantolactone	501:513	alantolactone (Ala)	501:519	Herein, we identified alantolactone (Ala) and dehydrodiisoeugenol (Deh) as the active anti-inflammatory components of F4-6 by UPLC-qTof MS analysis.					
36183605	4	29	theme	active	558:563	arg1	dehydrodiisoeugenol					525:543	dehydrodiisoeugenol	525:543	dehydrodiisoeugenol (Deh)	525:549	Herein, we identified alantolactone (Ala) and dehydrodiisoeugenol (Deh) as the active anti-inflammatory components of F4-6 by UPLC-qTof MS analysis.					
36183605	0	30	theme	action	118:123	arg1	mechanism					105:113	their mechanism	99:113	their mechanism of action in microglia	99:136	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	0	31	from	exploration	84:94	arg1	Wurile					73:78	Wurile	73:78	Wurile	73:78	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	9	32	theme	composition	1499:1509	arg1	optimization					1472:1483	the optimization	1468:1483	the optimization of the herbal composition for neuroprotection	1468:1529	Collectively, our data identified and quantified the key chemicals of EW and provide insights into the optimization of the herbal composition for neuroprotection.					
36183605	4	33	theme	anti-inflammatory	565:581	arg1	components					583:592	the active anti-inflammatory components	554:592	the active anti-inflammatory components of F4-6	554:600	Herein, we identified alantolactone (Ala) and dehydrodiisoeugenol (Deh) as the active anti-inflammatory components of F4-6 by UPLC-qTof MS analysis.					
36183605	4	33	theme	anti-inflammatory	565:581	arg1	alantolactone					501:513	alantolactone	501:513	alantolactone (Ala)	501:519	Herein, we identified alantolactone (Ala) and dehydrodiisoeugenol (Deh) as the active anti-inflammatory components of F4-6 by UPLC-qTof MS analysis.					
36183605	4	33	theme	anti-inflammatory	565:581	arg1	dehydrodiisoeugenol					525:543	dehydrodiisoeugenol	525:543	dehydrodiisoeugenol (Deh)	525:549	Herein, we identified alantolactone (Ala) and dehydrodiisoeugenol (Deh) as the active anti-inflammatory components of F4-6 by UPLC-qTof MS analysis.					
36183605	1	34	theme	poststroke	198:207	arg1	neuro-recovery					209:222	poststroke neuro-recovery	198:222	poststroke neuro-recovery	198:222	Ethnomedicine Eerdun Wurile (EW) can significantly promote poststroke neuro-recovery through modulation of microglia polarization.					
36183605	0	35	theme	anti-inflammatory	35:51	arg1	molecules					53:61	key anti-inflammatory molecules	31:61	key anti-inflammatory molecules in Eerdun Wurile	31:78	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	7	36	theme	treated	1001:1007	arg1	microglia					1009:1017	F4-6 treated microglia	996:1017	F4-6 treated microglia	996:1017	Moreover, the conditioned culture media obtained from F4-6 treated microglia significantly enhanced proliferation of N2a cells, and promoted neurite outgrowth possibly through upregulation of Nefh and Dlg4.					
36183605	5	37	theme	Deh	685:687	arg1	F4-6					647:650	F4-6	647:650	F4-6	647:650	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	37	theme	Deh	685:687	arg1	mixture					666:672	mixture	666:672	mixture of Ala and Deh (Mix)	666:693	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	37	theme	Deh	685:687	arg1	Deh					658:660	Deh	658:660	Deh	658:660	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	5	37	theme	Deh	685:687	arg1	Ala					653:655	Ala	653:655	Ala	653:655	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	8	38	theme	p65	1218:1220	arg1	expression					1198:1207	the expression	1194:1207	the expression of NF-κB p65	1194:1220	Mechanistically, F4-6 strongly downregulated the expression of NF-κB p65, while also inhibiting the nuclear translocation of p65, leading to the suppression of transcription of pro-inflammatory genes initiated by NF-κB.					
36183605	0	39	theme	key	31:33	arg1	molecules					53:61	key anti-inflammatory molecules	31:61	key anti-inflammatory molecules in Eerdun Wurile	31:78	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	2	40	attach	isolated	290:297	arg2	F4-6					284:287	F4-6	284:287	F4-6	284:287	Fraction 4-6 (F4-6) isolated from EW via serial fractionation inhibits the expression of pro-inflammatory genes in LPS stimulated microglia.					
36183605	2	40	attach	isolated	290:297	arg2	Fraction					270:277	Fraction 4-6	270:281	Fraction 4-6 (F4-6) isolated from EW via serial fractionation	270:330	Fraction 4-6 (F4-6) isolated from EW via serial fractionation inhibits the expression of pro-inflammatory genes in LPS stimulated microglia.					
36183605	2	40	attach	isolated	290:297	arg1	EW					304:305	EW	304:305	EW	304:305	Fraction 4-6 (F4-6) isolated from EW via serial fractionation inhibits the expression of pro-inflammatory genes in LPS stimulated microglia.					
36183605	3	41	theme	active	428:433	arg1	molecules					435:443	the key active molecules	420:443	the key active molecules of F4-6	420:451	However, the key active molecules of F4-6 have not been identified.					
36183605	0	42	from	Wurile	73:78	arg1	identification					13:26	identification	13:26	identification	13:26	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	0	42	from	Wurile	73:78	arg1	Analysis					0:7	Analysis	0:7	Analysis	0:7	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	7	43	theme	conditioned	956:966	arg1	media					976:980	the conditioned culture media	952:980	the conditioned culture media obtained from F4-6 treated microglia	952:1017	Moreover, the conditioned culture media obtained from F4-6 treated microglia significantly enhanced proliferation of N2a cells, and promoted neurite outgrowth possibly through upregulation of Nefh and Dlg4.					
36183605	7	44	theme	culture	968:974	arg1	media					976:980	the conditioned culture media	952:980	the conditioned culture media obtained from F4-6 treated microglia	952:1017	Moreover, the conditioned culture media obtained from F4-6 treated microglia significantly enhanced proliferation of N2a cells, and promoted neurite outgrowth possibly through upregulation of Nefh and Dlg4.					
36183605	0	45	theme	molecules	53:61	arg1	identification					13:26	identification	13:26	identification	13:26	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	0	45	theme	molecules	53:61	arg1	Analysis					0:7	Analysis	0:7	Analysis	0:7	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	6	46	theme	genes	897:901	arg1	expression					865:874	the expression	861:874	the expression of anti-inflammatory genes including Hmox1, Tgfβ, Igf1 and Creb1	861:939	At the same time upregulate the expression of anti-inflammatory genes including Hmox1, Tgfβ, Igf1 and Creb1.					
36183605	7	47	theme	F4-6	996:999	arg1	microglia					1009:1017	F4-6 treated microglia	996:1017	F4-6 treated microglia	996:1017	Moreover, the conditioned culture media obtained from F4-6 treated microglia significantly enhanced proliferation of N2a cells, and promoted neurite outgrowth possibly through upregulation of Nefh and Dlg4.					
36183605	7	48	theme	cells	1063:1067	arg1	proliferation					1042:1054	proliferation	1042:1054	proliferation of N2a cells	1042:1067	Moreover, the conditioned culture media obtained from F4-6 treated microglia significantly enhanced proliferation of N2a cells, and promoted neurite outgrowth possibly through upregulation of Nefh and Dlg4.					
36183605	8	49	theme	nuclear	1249:1255	arg1	translocation					1257:1269	the nuclear translocation	1245:1269	the nuclear translocation of p65	1245:1276	Mechanistically, F4-6 strongly downregulated the expression of NF-κB p65, while also inhibiting the nuclear translocation of p65, leading to the suppression of transcription of pro-inflammatory genes initiated by NF-κB.					
36183605	6	50	theme	anti-inflammatory	879:895	arg1	Creb1					935:939	Creb1	935:939	Creb1	935:939	At the same time upregulate the expression of anti-inflammatory genes including Hmox1, Tgfβ, Igf1 and Creb1.					
36183605	6	50	theme	anti-inflammatory	879:895	arg1	Tgfβ					920:923	Tgfβ	920:923	Tgfβ	920:923	At the same time upregulate the expression of anti-inflammatory genes including Hmox1, Tgfβ, Igf1 and Creb1.					
36183605	6	50	theme	anti-inflammatory	879:895	arg1	Hmox1					913:917	Hmox1	913:917	Hmox1	913:917	At the same time upregulate the expression of anti-inflammatory genes including Hmox1, Tgfβ, Igf1 and Creb1.					
36183605	6	50	theme	anti-inflammatory	879:895	arg1	genes					897:901	anti-inflammatory genes	879:901	anti-inflammatory genes including Hmox1, Tgfβ, Igf1 and Creb1	879:939	At the same time upregulate the expression of anti-inflammatory genes including Hmox1, Tgfβ, Igf1 and Creb1.					
36183605	6	50	theme	anti-inflammatory	879:895	arg1	Igf1					926:929	Igf1	926:929	Igf1	926:929	At the same time upregulate the expression of anti-inflammatory genes including Hmox1, Tgfβ, Igf1 and Creb1.					
36183605	0	51	from	mechanism	105:113	arg1	microglia					128:136	microglia	128:136	microglia	128:136	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	5	52	theme	genes	751:755	arg1	expression					712:721	the expression	708:721	the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia	708:809	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	7	53	theme	N2a	1059:1061	arg1	cells					1063:1067	N2a cells	1059:1067	N2a cells	1059:1067	Moreover, the conditioned culture media obtained from F4-6 treated microglia significantly enhanced proliferation of N2a cells, and promoted neurite outgrowth possibly through upregulation of Nefh and Dlg4.					
36183605	8	54	theme	p65	1274:1276	arg1	translocation					1257:1269	the nuclear translocation	1245:1269	the nuclear translocation of p65	1245:1276	Mechanistically, F4-6 strongly downregulated the expression of NF-κB p65, while also inhibiting the nuclear translocation of p65, leading to the suppression of transcription of pro-inflammatory genes initiated by NF-κB.					
36183605	4	55	theme	UPLC-qTof	605:613	arg1	analysis					618:625	UPLC-qTof MS analysis	605:625	UPLC-qTof MS analysis	605:625	Herein, we identified alantolactone (Ala) and dehydrodiisoeugenol (Deh) as the active anti-inflammatory components of F4-6 by UPLC-qTof MS analysis.					
36183605	8	56	theme	pro-inflammatory	1326:1341	arg1	genes					1343:1347	pro-inflammatory genes	1326:1347	pro-inflammatory genes initiated by NF-κB	1326:1366	Mechanistically, F4-6 strongly downregulated the expression of NF-κB p65, while also inhibiting the nuclear translocation of p65, leading to the suppression of transcription of pro-inflammatory genes initiated by NF-κB.					
36183605	0	57	from	microglia	128:136	arg1	mechanism					105:113	their mechanism	99:113	their mechanism of action in microglia	99:136	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
36183605	5	58	from	expression	712:721	arg1	microglia					801:809	LPS-treated microglia	789:809	LPS-treated microglia	789:809	We confirmed that, F4-6, Ala, Deh and mixture of Ala and Deh (Mix) downregulate the expression of several pro-inflammatory genes including Ccl2, Cox2 and Il6 in LPS-treated microglia in a similar pattern.					
36183605	1	59	theme	microglia	246:254	arg1	modulation					232:241	modulation	232:241	modulation of microglia polarization	232:267	Ethnomedicine Eerdun Wurile (EW) can significantly promote poststroke neuro-recovery through modulation of microglia polarization.					
36183605	4	60	theme	MS	615:616	arg1	analysis					618:625	UPLC-qTof MS analysis	605:625	UPLC-qTof MS analysis	605:625	Herein, we identified alantolactone (Ala) and dehydrodiisoeugenol (Deh) as the active anti-inflammatory components of F4-6 by UPLC-qTof MS analysis.					
36183605	0	61	from	action	118:123	arg1	microglia					128:136	microglia	128:136	microglia	128:136	Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia.					
35417879	0	0	theme	CARRIER	89:95	arg1	DEVELOPMENT					43:53	THE EXPERIMENTAL DEVELOPMENT	26:53	THE EXPERIMENTAL DEVELOPMENT OF A BIODEGRADABLE CHITOSAN BASED CARRIER	26:95	SCIENCE-BASED APPROACH TO THE EXPERIMENTAL DEVELOPMENT OF A BIODEGRADABLE CHITOSAN BASED CARRIER.					
35417879	3	1	theme	chitosan	661:668	arg1	forms					652:656	salt forms	647:656	salt forms of chitosan	647:668	A scientifically based approach to research has made it possible to develop a technology for obtaining a water-soluble biodegradable carrier based on salt forms of chitosan.					
35417879	6	2	theme	chitosan	1432:1439	arg1	ascorbate					1441:1449	chitosan ascorbate	1432:1449	chitosan ascorbate with the following parameters	1432:1479	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	1	3	theme	science-based	136:148	arg1	approach					150:157	a science-based approach	134:157	a science-based approach to the development of a carrier of a pharmaceutical substance	134:219	The aim of the study was to develop a science-based approach to the development of a carrier of a pharmaceutical substance using the modification of chitosan.					
35417879	0	4	theme	BASED	83:87	arg1	CARRIER					89:95	A BIODEGRADABLE CHITOSAN BASED CARRIER	58:95	A BIODEGRADABLE CHITOSAN BASED CARRIER	58:95	SCIENCE-BASED APPROACH TO THE EXPERIMENTAL DEVELOPMENT OF A BIODEGRADABLE CHITOSAN BASED CARRIER.					
35417879	3	5	theme	salt	647:650	arg1	forms					652:656	salt forms	647:656	salt forms of chitosan	647:668	A scientifically based approach to research has made it possible to develop a technology for obtaining a water-soluble biodegradable carrier based on salt forms of chitosan.					
35417879	6	6	theme	°C	1534:1535	arg1	temperature					1510:1520	a temperature	1508:1520	a temperature of minus 80 °C	1508:1535	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	6	7	dep	established	1394:1404	arg1	temperature					1648:1658	condenser temperature	1638:1658	condenser temperature	1638:1658	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	6	7	dep	established	1394:1404	arg1	80°C					1666:1669	80°C	1666:1669	80°C	1666:1669	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	6	7	dep	established	1394:1404	arg1	freezing					1482:1489	freezing	1482:1489	freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar	1482:1635	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	2	8	theme	ascorbic	324:331	arg1	acidity					314:320	acidity -	314:322	acidity - ascorbic and acetic	314:342	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	5	9	theme	obtained	1154:1161	arg1	solutions					1163:1171	the obtained solutions	1150:1171	the obtained solutions of salt forms of chitosan	1150:1197	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	2	10	theme	salt	486:489	arg1	form					491:494	its solid salt form	476:494	its solid salt form	476:494	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	5	11	theme	higher	1062:1067	arg1	pH					1103:1104	the pH	1099:1104	the pH of wound exudate	1099:1121	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	11	theme	higher	1062:1067	arg1	values					1072:1077	higher pH values	1062:1077	higher pH values	1062:1077	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	12	theme	dosage	1318:1323	arg1	form					1325:1328	the dosage form	1314:1328	the dosage form	1314:1328	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	13	theme	solutions	1163:1171	arg1	pH					1103:1104	the pH	1099:1104	the pH of wound exudate	1099:1121	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	13	theme	solutions	1163:1171	arg1	properties					1010:1019	the better organoleptic properties	986:1019	the better organoleptic properties compared to chitosan acetate	986:1048	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	13	theme	solutions	1163:1171	arg1	values					1072:1077	higher pH values	1062:1077	higher pH values	1062:1077	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	13	theme	solutions	1163:1171	arg1	viscosities					1135:1145	higher viscosities	1128:1145	higher viscosities of the obtained solutions of salt forms of chitosan	1128:1197	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	1	14	theme	chitosan	247:254	arg1	modification					231:242	the modification	227:242	the modification of chitosan	227:254	The aim of the study was to develop a science-based approach to the development of a carrier of a pharmaceutical substance using the modification of chitosan.					
35417879	2	15	theme	solid	480:484	arg1	form					491:494	its solid salt form	476:494	its solid salt form	476:494	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	4	16	theme	salt	954:957	arg1	forms					959:963	its salt forms	950:963	its salt forms	950:963	The experimental approach to research made it possible to establish the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms.					
35417879	5	17	theme	form	1325:1328	arg1	composition					1299:1309	the composition	1295:1309	the composition of the dosage form	1295:1328	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	18	theme	exudate	1115:1121	arg1	pH					1103:1104	the pH	1099:1104	the pH of wound exudate	1099:1121	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	18	theme	exudate	1115:1121	arg1	values					1072:1077	higher pH values	1062:1077	higher pH values	1062:1077	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	19	theme	chitosan	1033:1040	arg1	acetate					1042:1048	chitosan acetate	1033:1048	chitosan acetate	1033:1048	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	20	theme	forms	1181:1185	arg1	solutions					1163:1171	the obtained solutions	1150:1171	the obtained solutions of salt forms of chitosan	1150:1197	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	6	21	from	temperature	1510:1520	arg1	hours					1499:1503	2-3 hours	1495:1503	2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar	1495:1635	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	6	22	theme	post-drying	1586:1596	arg1	mode					1598:1601	post-drying mode	1586:1601	post-drying mode for 8 hours	1586:1613	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	4	23	theme	chitosan	927:934	arg1	form					919:922	the native form	908:922	the native form of chitosan	908:934	The experimental approach to research made it possible to establish the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms.					
35417879	6	24	theme	solid	1418:1422	arg1	form					1424:1427	a solid form	1416:1427	a solid form of chitosan ascorbate with the following parameters	1416:1479	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	2	25	theme	chitosan	408:415	arg1	modification					392:403	modification	392:403	modification of chitosan	392:415	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	4	26	theme	native	912:917	arg1	form					919:922	the native form	908:922	the native form of chitosan	908:934	The experimental approach to research made it possible to establish the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms.					
35417879	4	27	theme	transparent	794:804	arg1	solutions					806:814	transparent solutions	794:814	transparent solutions of chitosan	794:826	The experimental approach to research made it possible to establish the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms.					
35417879	2	28	theme	upper	299:303	arg1	limit					305:309	the upper limit	295:309	the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form	295:494	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	6	29	dep	hours	1499:1503	arg1	pressure					1616:1623	pressure	1616:1623	pressure	1616:1623	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	6	29	dep	hours	1499:1503	arg1	mode					1598:1601	post-drying mode	1586:1601	post-drying mode for 8 hours	1586:1613	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	6	29	dep	hours	1499:1503	arg1	pressure					1564:1571	pressure	1564:1571	pressure	1564:1571	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	6	29	dep	hours	1499:1503	arg1	mbar					1632:1635	0.12 mbar	1627:1635	0.12 mbar	1627:1635	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	6	29	dep	hours	1499:1503	arg1	mode					1545:1548	drying mode	1538:1548	drying mode for 40 hours	1538:1561	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	6	29	dep	hours	1499:1503	arg1	mbar					1580:1583	0.34 mbar	1575:1583	0.34 mbar	1575:1583	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	2	30	theme	salt	354:357	arg1	forms					359:363	salt forms	354:363	salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form	354:494	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	0	31	theme	SCIENCE-BASED	0:12	arg1	APPROACH					14:21	SCIENCE-BASED APPROACH	0:21	SCIENCE-BASED APPROACH	0:21	SCIENCE-BASED APPROACH TO THE EXPERIMENTAL DEVELOPMENT OF A BIODEGRADABLE CHITOSAN BASED CARRIER.					
35417879	6	32	dep	°C	1534:1535	arg1	80					1531:1532	80	1531:1532	80	1531:1532	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	6	33	theme	laboratory	1368:1377	arg1	conditions					1379:1388	laboratory conditions	1368:1388	laboratory conditions	1368:1388	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	5	34	theme	composition	1299:1309	arg1	development					1280:1290	further development	1272:1290	further development of the composition of the dosage form	1272:1328	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	35	theme	chitosan	1190:1197	arg1	forms					1181:1185	salt forms	1176:1185	salt forms of chitosan	1176:1197	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	36	theme	organoleptic	997:1008	arg1	properties					1010:1019	the better organoleptic properties	986:1019	the better organoleptic properties compared to chitosan acetate	986:1048	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	2	37	theme	modification	392:403	arg1	development					438:448	the experimental development	421:448	the experimental development of a method for obtaining its solid salt form	421:494	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	2	37	theme	modification	392:403	arg1	chitosan					368:375	chitosan	368:375	chitosan	368:375	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	2	37	theme	modification	392:403	arg1	choice					382:387	the choice	378:387	the choice of modification of chitosan	378:415	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	6	38	theme	0.12	1627:1630	arg1	mbar					1632:1635	0.12 mbar	1627:1635	0.12 mbar	1627:1635	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	4	39	theme	ascorbic	858:865	arg1	acids					867:871	acetic and ascorbic acids	847:871	acetic and ascorbic acids	847:871	The experimental approach to research made it possible to establish the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms.					
35417879	3	40	theme	based	514:518	arg1	approach					520:527	A scientifically based approach	497:527	A scientifically based approach to research	497:539	A scientifically based approach to research has made it possible to develop a technology for obtaining a water-soluble biodegradable carrier based on salt forms of chitosan.					
35417879	6	41	theme	optimal	1335:1341	arg1	mode					1343:1346	The optimal mode	1331:1346	The optimal mode of freeze-drying in laboratory conditions	1331:1388	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	2	42	theme	choice	382:387	arg1	forms					359:363	salt forms	354:363	salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form	354:494	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	1	43	theme	carrier	183:189	arg1	development					166:176	the development	162:176	the development of a carrier of a pharmaceutical substance	162:219	The aim of the study was to develop a science-based approach to the development of a carrier of a pharmaceutical substance using the modification of chitosan.					
35417879	2	44	theme	development	438:448	arg1	forms					359:363	salt forms	354:363	salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form	354:494	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	2	45	theme	acidity	314:320	arg1	limit					305:309	the upper limit	295:309	the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form	295:494	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	3	46	theme	water-soluble	602:614	arg1	carrier					630:636	a water-soluble biodegradable carrier	600:636	a water-soluble biodegradable carrier based on salt forms of chitosan	600:668	A scientifically based approach to research has made it possible to develop a technology for obtaining a water-soluble biodegradable carrier based on salt forms of chitosan.					
35417879	5	47	dep	form	1242:1245	arg1	ascorbate					1258:1266	ascorbate	1258:1266	ascorbate	1258:1266	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	0	48	theme	EXPERIMENTAL	30:41	arg1	DEVELOPMENT					43:53	THE EXPERIMENTAL DEVELOPMENT	26:53	THE EXPERIMENTAL DEVELOPMENT OF A BIODEGRADABLE CHITOSAN BASED CARRIER	26:95	SCIENCE-BASED APPROACH TO THE EXPERIMENTAL DEVELOPMENT OF A BIODEGRADABLE CHITOSAN BASED CARRIER.					
35417879	5	49	theme	pH	1069:1070	arg1	pH					1103:1104	the pH	1099:1104	the pH of wound exudate	1099:1121	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	5	49	theme	pH	1069:1070	arg1	values					1072:1077	higher pH values	1062:1077	higher pH values	1062:1077	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	2	50	theme	experimental	425:436	arg1	development					438:448	the experimental development	421:448	the experimental development of a method for obtaining its solid salt form	421:494	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	4	51	theme	acidity	762:768	arg1	limit					749:753	the upper limit	739:753	the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms	739:963	The experimental approach to research made it possible to establish the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms.					
35417879	2	52	theme	chitosan	368:375	arg1	forms					359:363	salt forms	354:363	salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form	354:494	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	6	53	with	ascorbate	1441:1449	arg1	parameters					1470:1479	the following parameters	1456:1479	the following parameters	1456:1479	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	0	54	theme	BIODEGRADABLE	60:72	arg1	CARRIER					89:95	A BIODEGRADABLE CHITOSAN BASED CARRIER	58:95	A BIODEGRADABLE CHITOSAN BASED CARRIER	58:95	SCIENCE-BASED APPROACH TO THE EXPERIMENTAL DEVELOPMENT OF A BIODEGRADABLE CHITOSAN BASED CARRIER.					
35417879	4	55	theme	medium	777:782	arg1	acidity					762:768	the acidity	758:768	the acidity	758:768	The experimental approach to research made it possible to establish the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms.					
35417879	6	56	theme	condenser	1638:1646	arg1	temperature					1648:1658	condenser temperature	1638:1658	condenser temperature	1638:1658	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	6	57	theme	drying	1538:1543	arg1	mode					1545:1548	drying mode	1538:1548	drying mode for 40 hours	1538:1561	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	5	58	theme	further	1272:1278	arg1	development					1280:1290	further development	1272:1290	further development of the composition of the dosage form	1272:1328	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	1	59	theme	study	113:117	arg1	aim					102:104	The aim	98:104	The aim of the study	98:117	The aim of the study was to develop a science-based approach to the development of a carrier of a pharmaceutical substance using the modification of chitosan.					
35417879	4	60	theme	acetic	847:852	arg1	acids					867:871	acetic and ascorbic acids	847:871	acetic and ascorbic acids	847:871	The experimental approach to research made it possible to establish the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms.					
35417879	5	61	theme	wound	1109:1113	arg1	exudate					1115:1121	wound exudate	1109:1121	wound exudate	1109:1121	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	6	62	theme	following	1460:1468	arg1	parameters					1470:1479	the following parameters	1456:1479	the following parameters	1456:1479	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	5	63	theme	salt	1176:1179	arg1	forms					1181:1185	salt forms	1176:1185	salt forms of chitosan	1176:1197	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	1	64	theme	pharmaceutical	196:209	arg1	substance					211:219	a pharmaceutical substance	194:219	a pharmaceutical substance	194:219	The aim of the study was to develop a science-based approach to the development of a carrier of a pharmaceutical substance using the modification of chitosan.					
35417879	3	65	theme	biodegradable	616:628	arg1	carrier					630:636	a water-soluble biodegradable carrier	600:636	a water-soluble biodegradable carrier based on salt forms of chitosan	600:668	A scientifically based approach to research has made it possible to develop a technology for obtaining a water-soluble biodegradable carrier based on salt forms of chitosan.					
35417879	4	66	theme	upper	743:747	arg1	limit					749:753	the upper limit	739:753	the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms	739:963	The experimental approach to research made it possible to establish the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms.					
35417879	5	67	theme	water-soluble	1228:1240	arg1	form					1242:1245	a water-soluble form	1226:1245	a water-soluble form	1226:1245	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	4	68	theme	experimental	675:686	arg1	approach					688:695	The experimental approach	671:695	The experimental approach to research	671:707	The experimental approach to research made it possible to establish the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms.					
35417879	4	69	theme	chitosan	819:826	arg1	solutions					806:814	transparent solutions	794:814	transparent solutions of chitosan	794:826	The experimental approach to research made it possible to establish the upper limit of the acidity of the medium, at which transparent solutions of chitosan were obtained, for acetic and ascorbic acids, which are necessary for modifying the native form of chitosan and obtaining its salt forms.					
35417879	1	70	theme	substance	211:219	arg1	carrier					183:189	a carrier	181:189	a carrier of a pharmaceutical substance	181:219	The aim of the study was to develop a science-based approach to the development of a carrier of a pharmaceutical substance using the modification of chitosan.					
35417879	0	71	theme	CHITOSAN	74:81	arg1	CARRIER					89:95	A BIODEGRADABLE CHITOSAN BASED CARRIER	58:95	A BIODEGRADABLE CHITOSAN BASED CARRIER	58:95	SCIENCE-BASED APPROACH TO THE EXPERIMENTAL DEVELOPMENT OF A BIODEGRADABLE CHITOSAN BASED CARRIER.					
35417879	0	72	dep	APPROACH	14:21	arg1	DEVELOPMENT					43:53	THE EXPERIMENTAL DEVELOPMENT	26:53	THE EXPERIMENTAL DEVELOPMENT OF A BIODEGRADABLE CHITOSAN BASED CARRIER	26:95	SCIENCE-BASED APPROACH TO THE EXPERIMENTAL DEVELOPMENT OF A BIODEGRADABLE CHITOSAN BASED CARRIER.					
35417879	6	73	theme	freeze-drying	1351:1363	arg1	mode					1343:1346	The optimal mode	1331:1346	The optimal mode of freeze-drying in laboratory conditions	1331:1388	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	5	74	theme	higher	1128:1133	arg1	viscosities					1135:1145	higher viscosities	1128:1145	higher viscosities of the obtained solutions of salt forms of chitosan	1128:1197	Taking into account the better organoleptic properties compared to chitosan acetate, as well as higher pH values, which are close to the pH of wound exudate, and higher viscosities of the obtained solutions of salt forms of chitosan, it was possible to select a water-soluble form - chitosan ascorbate for further development of the composition of the dosage form.					
35417879	2	75	theme	method	455:460	arg1	development					438:448	the experimental development	421:448	the experimental development of a method for obtaining its solid salt form	421:494	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	2	75	theme	method	455:460	arg1	chitosan					368:375	chitosan	368:375	chitosan	368:375	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	2	75	theme	method	455:460	arg1	choice					382:387	the choice	378:387	the choice of modification of chitosan	378:415	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
35417879	6	76	from	mode	1343:1346	arg1	conditions					1379:1388	laboratory conditions	1368:1388	laboratory conditions	1368:1388	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	6	77	theme	ascorbate	1441:1449	arg1	form					1424:1427	a solid form	1416:1427	a solid form of chitosan ascorbate with the following parameters	1416:1479	The optimal mode of freeze-drying in laboratory conditions was established to obtain a solid form of chitosan ascorbate with the following parameters: freezing for 2-3 hours at a temperature of minus 80 °C; drying mode for 40 hours, pressure - 0.34 mbar; post-drying mode for 8 hours, pressure - 0.12 mbar; condenser temperature minus 80°C.					
35417879	2	78	theme	acetic	337:342	arg1	acidity					314:320	acidity -	314:322	acidity - ascorbic and acetic	314:342	Studies were carried out to determine the upper limit of acidity - ascorbic and acetic to obtain salt forms of chitosan, the choice of modification of chitosan and the experimental development of a method for obtaining its solid salt form.					
37269692	2	0	theme	traditional	454:464	arg1	extraction					474:483	traditional solvent extraction	454:483	traditional solvent extraction	454:483	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	3	1	from	α-tocopherol	630:641	arg1	rich					577:580	rich	577:580	rich	577:580	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	9	2	theme	fruits	1633:1638	arg1	utilization					1611:1621	the comprehensive utilization	1593:1621	the comprehensive utilization of cactus fruits	1593:1638	These findings provide new insights into the comprehensive utilization of cactus fruits.					
37269692	3	3	theme	20.01 ± 1.86 mg/100 g	644:664	arg1	α-tocopherol					630:641	α-tocopherol	630:641	α-tocopherol (20.01 ± 1.86 mg/100 g oil)	630:669	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	3	3	theme	20.01 ± 1.86 mg/100 g	644:664	arg1	oil					666:668	20.01 ± 1.86 mg/100 g oil	644:668	20.01 ± 1.86 mg/100 g oil	644:668	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	7	4	theme	Lipidomics	1213:1222	arg1	analysis					1224:1231	Lipidomics analysis	1213:1231	Lipidomics analysis	1213:1231	Lipidomics analysis showed that CFO significantly reduced the content of oxidized phospholipids stimulated by LPS and increased the content of highly bioactive metabolites such as ceramides, thus alleviating LPS-induced damage in C. elegans.					
37269692	0	5	theme	cactus	92:97	arg1	oil					133:135	cactus fruit (Opuntia ficus-indica) seed oil	92:135	cactus fruit (Opuntia ficus-indica) seed oil	92:135	Foodomics revealed the effects of ultrasonic extraction on the composition and nutrition of cactus fruit (Opuntia ficus-indica) seed oil.					
37269692	4	6	theme	ultrasound-assisted	750:768	arg1	extraction					770:779	ultrasound-assisted extraction	750:779	ultrasound-assisted extraction	750:779	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	4	7	theme	ultrasound	868:877	arg1	intensity					879:887	excessive ultrasound intensity	858:887	excessive ultrasound intensity	858:887	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	2	8	dep	composition	328:338	arg1	the					324:326	the	324:326	the	324:326	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	6	9	used	used	1207:1210	arg2	model					1197:1201	a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model	1135:1201	a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model	1135:1201	To further demonstrate the nutritional value of CFO, a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model was used.					
37269692	4	10	theme	excessive	858:866	arg1	intensity					879:887	excessive ultrasound intensity	858:887	excessive ultrasound intensity	858:887	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	6	11	theme	lipid	1170:1174	arg1	metabolism					1176:1185	lipid metabolism	1170:1185	a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model	1135:1201	To further demonstrate the nutritional value of CFO, a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model was used.					
37269692	4	12	theme	traditional	718:728	arg1	extraction					738:747	traditional solvent extraction	718:747	traditional solvent extraction	718:747	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	2	13	theme	oil	488:490	arg1	quality					492:498	oil quality	488:498	oil quality	488:498	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	2	14	from	effects	408:414	arg1	quality					492:498	oil quality	488:498	oil quality	488:498	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	4	15	theme	concomitants	829:840	arg1	content					812:818	the content	808:818	the content of lipid concomitants in CFO	808:847	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	7	16	theme	oxidized	1286:1293	arg1	phospholipids					1295:1307	oxidized phospholipids	1286:1307	oxidized phospholipids stimulated by LPS	1286:1325	Lipidomics analysis showed that CFO significantly reduced the content of oxidized phospholipids stimulated by LPS and increased the content of highly bioactive metabolites such as ceramides, thus alleviating LPS-induced damage in C. elegans.					
37269692	3	17	theme	Foodomics	501:509	arg1	analysis					511:518	Foodomics analysis	501:518	Foodomics analysis	501:518	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	8	18	with	oil	1482:1484	arg1	value					1496:1500	high value	1491:1500	high value	1491:1500	Hence, CFO is a functional oil with high value, and ultrasound-assisted extraction is advocated.					
37269692	2	19	theme	fruit	372:376	arg1	oil					383:385	cactus fruit seed oil	365:385	cactus fruit seed oil (CFO)	365:391	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	2	19	theme	fruit	372:376	arg1	CFO					388:390	CFO	388:390	CFO	388:390	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	0	20	theme	fruit	99:103	arg1	oil					133:135	cactus fruit (Opuntia ficus-indica) seed oil	92:135	cactus fruit (Opuntia ficus-indica) seed oil	92:135	Foodomics revealed the effects of ultrasonic extraction on the composition and nutrition of cactus fruit (Opuntia ficus-indica) seed oil.					
37269692	2	21	theme	extraction	439:448	arg1	effects					408:414	the effects	404:414	the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality	404:498	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	3	22	from	acid	595:598	arg1	rich					577:580	rich	577:580	rich	577:580	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	1	23	theme	tropical	150:157	arg1	fruit					159:163	a tropical fruit	148:163	a tropical fruit with a high nutritional value	148:193	Cactus is a tropical fruit with a high nutritional value; however, little information is available regarding the comprehensive utilization of its byproducts.					
37269692	1	23	theme	tropical	150:157	arg1	Cactus					138:143	Cactus	138:143	Cactus	138:143	Cactus is a tropical fruit with a high nutritional value; however, little information is available regarding the comprehensive utilization of its byproducts.					
37269692	4	24	theme	radicals	949:956	arg1	formation					931:939	the formation	927:939	the formation of free radicals	927:956	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	4	24	theme	radicals	949:956	arg1	oxidation					905:913	the oxidation	901:913	the oxidation of oils	901:921	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	5	25	theme	CFO	1077:1079	arg1	crystallization					1038:1052	crystallization	1038:1052	crystallization	1038:1052	Analysis of the thermal properties showed that ultrasound had no effect on the crystallization or melting behavior of CFO.					
37269692	5	25	theme	CFO	1077:1079	arg1	behavior					1065:1072	melting behavior	1057:1072	melting behavior	1057:1072	Analysis of the thermal properties showed that ultrasound had no effect on the crystallization or melting behavior of CFO.					
37269692	7	26	theme	phospholipids	1295:1307	arg1	content					1275:1281	the content	1271:1281	the content of oxidized phospholipids stimulated by LPS	1271:1325	Lipidomics analysis showed that CFO significantly reduced the content of oxidized phospholipids stimulated by LPS and increased the content of highly bioactive metabolites such as ceramides, thus alleviating LPS-induced damage in C. elegans.					
37269692	3	27	from	canolol	676:682	arg1	rich					577:580	rich	577:580	rich	577:580	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	4	28	theme	oils	918:921	arg1	formation					931:939	the formation	927:939	the formation of free radicals	927:956	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	4	28	theme	oils	918:921	arg1	oxidation					905:913	the oxidation	901:913	the oxidation of oils	901:921	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	6	29	theme	CFO	1130:1132	arg1	value					1121:1125	the nutritional value	1105:1125	the nutritional value of CFO	1105:1132	To further demonstrate the nutritional value of CFO, a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model was used.					
37269692	7	30	theme	LPS-induced	1421:1431	arg1	damage					1433:1438	LPS-induced damage	1421:1438	LPS-induced damage	1421:1438	Lipidomics analysis showed that CFO significantly reduced the content of oxidized phospholipids stimulated by LPS and increased the content of highly bioactive metabolites such as ceramides, thus alleviating LPS-induced damage in C. elegans.					
37269692	1	31	theme	comprehensive	251:263	arg1	utilization					265:275	the comprehensive utilization	247:275	the comprehensive utilization of its byproducts	247:293	Cactus is a tropical fruit with a high nutritional value; however, little information is available regarding the comprehensive utilization of its byproducts.					
37269692	6	32	theme	imbalance	1187:1195	arg1	model					1197:1201	a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model	1135:1201	a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model	1135:1201	To further demonstrate the nutritional value of CFO, a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model was used.					
37269692	0	33	dep	composition	63:73	arg1	the					59:61	the	59:61	the	59:61	Foodomics revealed the effects of ultrasonic extraction on the composition and nutrition of cactus fruit (Opuntia ficus-indica) seed oil.					
37269692	8	34	theme	functional	1471:1480	arg1	oil					1482:1484	a functional oil	1469:1484	a functional oil with high value	1469:1500	Hence, CFO is a functional oil with high value, and ultrasound-assisted extraction is advocated.					
37269692	8	34	theme	functional	1471:1480	arg1	CFO					1462:1464	CFO	1462:1464	CFO	1462:1464	Hence, CFO is a functional oil with high value, and ultrasound-assisted extraction is advocated.					
37269692	8	35	theme	high	1491:1494	arg1	value					1496:1500	high value	1491:1500	high value	1491:1500	Hence, CFO is a functional oil with high value, and ultrasound-assisted extraction is advocated.					
37269692	6	36	theme	-induced	1161:1168	arg1	model					1197:1201	a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model	1135:1201	a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model	1135:1201	To further demonstrate the nutritional value of CFO, a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model was used.					
37269692	1	37	theme	high	172:175	arg1	value					189:193	a high nutritional value	170:193	a high nutritional value	170:193	Cactus is a tropical fruit with a high nutritional value; however, little information is available regarding the comprehensive utilization of its byproducts.					
37269692	0	38	theme	ultrasonic	34:43	arg1	extraction					45:54	ultrasonic extraction	34:54	ultrasonic extraction	34:54	Foodomics revealed the effects of ultrasonic extraction on the composition and nutrition of cactus fruit (Opuntia ficus-indica) seed oil.					
37269692	0	39	theme	seed	128:131	arg1	oil					133:135	cactus fruit (Opuntia ficus-indica) seed oil	92:135	cactus fruit (Opuntia ficus-indica) seed oil	92:135	Foodomics revealed the effects of ultrasonic extraction on the composition and nutrition of cactus fruit (Opuntia ficus-indica) seed oil.					
37269692	1	40	theme	nutritional	177:187	arg1	value					189:193	a high nutritional value	170:193	a high nutritional value	170:193	Cactus is a tropical fruit with a high nutritional value; however, little information is available regarding the comprehensive utilization of its byproducts.					
37269692	7	41	theme	bioactive	1363:1371	arg1	ceramides					1393:1401	ceramides	1393:1401	ceramides	1393:1401	Lipidomics analysis showed that CFO significantly reduced the content of oxidized phospholipids stimulated by LPS and increased the content of highly bioactive metabolites such as ceramides, thus alleviating LPS-induced damage in C. elegans.					
37269692	7	41	theme	bioactive	1363:1371	arg1	metabolites					1373:1383	highly bioactive metabolites	1356:1383	highly bioactive metabolites such as ceramides	1356:1401	Lipidomics analysis showed that CFO significantly reduced the content of oxidized phospholipids stimulated by LPS and increased the content of highly bioactive metabolites such as ceramides, thus alleviating LPS-induced damage in C. elegans.					
37269692	3	42	from	rich	577:580	arg1	canolol					676:682	canolol	676:682	canolol (200.10 ± 1.21 μg/g)	676:703	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	3	42	from	rich	577:580	arg1	200.10 ± 1.21 μg/g					685:702	200.10 ± 1.21 μg/g	685:702	200.10 ± 1.21 μg/g	685:702	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	3	42	from	rich	577:580	arg1	α-tocopherol					630:641	α-tocopherol	630:641	α-tocopherol (20.01 ± 1.86 mg/100 g oil)	630:669	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	3	42	from	rich	577:580	arg1	oil					666:668	20.01 ± 1.86 mg/100 g oil	644:668	20.01 ± 1.86 mg/100 g oil	644:668	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	3	42	from	rich	577:580	arg1	acid					595:598	linolenic acid	585:598	linolenic acid (9c12cC18:2, 57.46 ± 0.84 %)	585:627	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	2	43	theme	oil	383:385	arg1	composition					328:338	composition	328:338	composition	328:338	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	2	43	theme	oil	383:385	arg1	value					356:360	nutritional value	344:360	nutritional value	344:360	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	2	44	theme	ultrasound-assisted	419:437	arg1	extraction					439:448	ultrasound-assisted extraction	419:448	ultrasound-assisted extraction	419:448	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	5	45	theme	melting	1057:1063	arg1	behavior					1065:1072	melting behavior	1057:1072	melting behavior	1057:1072	Analysis of the thermal properties showed that ultrasound had no effect on the crystallization or melting behavior of CFO.					
37269692	7	46	theme	metabolites	1373:1383	arg1	content					1345:1351	the content	1341:1351	the content of highly bioactive metabolites such as ceramides	1341:1401	Lipidomics analysis showed that CFO significantly reduced the content of oxidized phospholipids stimulated by LPS and increased the content of highly bioactive metabolites such as ceramides, thus alleviating LPS-induced damage in C. elegans.					
37269692	2	47	theme	seed	378:381	arg1	oil					383:385	cactus fruit seed oil	365:385	cactus fruit seed oil (CFO)	365:391	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	2	47	theme	seed	378:381	arg1	CFO					388:390	CFO	388:390	CFO	388:390	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	1	48	theme	byproducts	284:293	arg1	utilization					265:275	the comprehensive utilization	247:275	the comprehensive utilization of its byproducts	247:293	Cactus is a tropical fruit with a high nutritional value; however, little information is available regarding the comprehensive utilization of its byproducts.					
37269692	1	49	with	fruit	159:163	arg1	value					189:193	a high nutritional value	170:193	a high nutritional value	170:193	Cactus is a tropical fruit with a high nutritional value; however, little information is available regarding the comprehensive utilization of its byproducts.					
37269692	4	50	theme	free	944:947	arg1	radicals					949:956	free radicals	944:956	free radicals	944:956	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	3	51	theme	traditional	554:564	arg1	solvent					566:572	a traditional solvent	552:572	a traditional solvent	552:572	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	4	52	from	content	812:818	arg1	CFO					845:847	CFO	845:847	CFO	845:847	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	6	53	theme	nutritional	1109:1119	arg1	value					1121:1125	the nutritional value	1105:1125	the nutritional value of CFO	1105:1132	To further demonstrate the nutritional value of CFO, a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model was used.					
37269692	0	54	theme	extraction	45:54	arg1	effects					23:29	the effects	19:29	the effects of ultrasonic extraction on the composition and nutrition of cactus fruit (Opuntia ficus-indica) seed oil	19:135	Foodomics revealed the effects of ultrasonic extraction on the composition and nutrition of cactus fruit (Opuntia ficus-indica) seed oil.					
37269692	3	55	dep	acid	595:598	arg1	9c12cC18:2					601:610	9c12cC18:2	601:610	9c12cC18:2	601:610	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	3	55	dep	acid	595:598	arg1	%					626:626	57.46 ± 0.84 %	613:626	57.46 ± 0.84 %	613:626	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	0	56	theme	oil	133:135	arg1	nutrition					79:87	nutrition	79:87	nutrition	79:87	Foodomics revealed the effects of ultrasonic extraction on the composition and nutrition of cactus fruit (Opuntia ficus-indica) seed oil.					
37269692	0	56	theme	oil	133:135	arg1	composition					63:73	composition	63:73	composition	63:73	Foodomics revealed the effects of ultrasonic extraction on the composition and nutrition of cactus fruit (Opuntia ficus-indica) seed oil.					
37269692	4	57	theme	lipid	823:827	arg1	concomitants					829:840	lipid concomitants	823:840	lipid concomitants	823:840	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	5	58	contain	had	1017:1019	arg1	ultrasound					1006:1015	ultrasound	1006:1015	ultrasound	1006:1015	Analysis of the thermal properties showed that ultrasound had no effect on the crystallization or melting behavior of CFO.					
37269692	5	58	contain	had	1017:1019	arg2	effect					1024:1029	no effect	1021:1029	no effect	1021:1029	Analysis of the thermal properties showed that ultrasound had no effect on the crystallization or melting behavior of CFO.					
37269692	5	59	theme	properties	983:992	arg1	Analysis					959:966	Analysis	959:966	Analysis of the thermal properties	959:992	Analysis of the thermal properties showed that ultrasound had no effect on the crystallization or melting behavior of CFO.					
37269692	9	60	theme	comprehensive	1597:1609	arg1	utilization					1611:1621	the comprehensive utilization	1593:1621	the comprehensive utilization of cactus fruits	1593:1638	These findings provide new insights into the comprehensive utilization of cactus fruits.					
37269692	6	61	theme	metabolism	1176:1185	arg1	model					1197:1201	a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model	1135:1201	a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model	1135:1201	To further demonstrate the nutritional value of CFO, a lipopolysaccharide (LPS)-induced lipid metabolism imbalance model was used.					
37269692	9	62	theme	cactus	1626:1631	arg1	fruits					1633:1638	cactus fruits	1626:1638	cactus fruits	1626:1638	These findings provide new insights into the comprehensive utilization of cactus fruits.					
37269692	4	63	theme	solvent	730:736	arg1	extraction					738:747	traditional solvent extraction	718:747	traditional solvent extraction	718:747	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	9	64	theme	new	1575:1577	arg1	insights					1579:1586	new insights	1575:1586	new insights into the comprehensive utilization of cactus fruits	1575:1638	These findings provide new insights into the comprehensive utilization of cactus fruits.					
37269692	0	65	dep	oil	133:135	arg1	ficus-indica					114:125	Opuntia ficus-indica	106:125	Opuntia ficus-indica	106:125	Foodomics revealed the effects of ultrasonic extraction on the composition and nutrition of cactus fruit (Opuntia ficus-indica) seed oil.					
37269692	2	66	theme	extraction	474:483	arg1	effects					408:414	the effects	404:414	the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality	404:498	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	4	67	dep	increase	799:806	arg1	whereas					850:856	whereas	850:856	whereas	850:856	Compared to traditional solvent extraction, ultrasound-assisted extraction can significantly increase the content of lipid concomitants in CFO, whereas excessive ultrasound intensity may lead to the oxidation of oils and the formation of free radicals.					
37269692	5	68	theme	thermal	975:981	arg1	properties					983:992	the thermal properties	971:992	the thermal properties	971:992	Analysis of the thermal properties showed that ultrasound had no effect on the crystallization or melting behavior of CFO.					
37269692	8	69	theme	ultrasound-assisted	1507:1525	arg1	extraction					1527:1536	ultrasound-assisted extraction	1507:1536	ultrasound-assisted extraction	1507:1536	Hence, CFO is a functional oil with high value, and ultrasound-assisted extraction is advocated.					
37269692	2	70	theme	nutritional	344:354	arg1	value					356:360	nutritional value	344:360	nutritional value	344:360	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	2	71	theme	cactus	365:370	arg1	oil					383:385	cactus fruit seed oil	365:385	cactus fruit seed oil (CFO)	365:391	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	2	71	theme	cactus	365:370	arg1	CFO					388:390	CFO	388:390	CFO	388:390	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	5	72	dep	crystallization	1038:1052	arg1	the					1034:1036	the	1034:1036	the	1034:1036	Analysis of the thermal properties showed that ultrasound had no effect on the crystallization or melting behavior of CFO.					
37269692	2	73	theme	solvent	466:472	arg1	extraction					474:483	traditional solvent extraction	454:483	traditional solvent extraction	454:483	This study aimed to explore the composition and nutritional value of cactus fruit seed oil (CFO) and reveal the effects of ultrasound-assisted extraction and traditional solvent extraction on oil quality.					
37269692	0	74	from	effects	23:29	arg1	nutrition					79:87	nutrition	79:87	nutrition	79:87	Foodomics revealed the effects of ultrasonic extraction on the composition and nutrition of cactus fruit (Opuntia ficus-indica) seed oil.					
37269692	0	74	from	effects	23:29	arg1	composition					63:73	composition	63:73	composition	63:73	Foodomics revealed the effects of ultrasonic extraction on the composition and nutrition of cactus fruit (Opuntia ficus-indica) seed oil.					
37269692	3	75	theme	linolenic	585:593	arg1	acid					595:598	linolenic acid	585:598	linolenic acid (9c12cC18:2, 57.46 ± 0.84 %)	585:627	Foodomics analysis showed that CFO extracted using a traditional solvent is rich in linolenic acid (9c12cC18:2, 57.46 ± 0.84 %), α-tocopherol (20.01 ± 1.86 mg/100 g oil), and canolol (200.10 ± 1.21 μg/g).					
37269692	1	76	theme	little	205:210	arg1	information					212:222	little information	205:222	little information	205:222	Cactus is a tropical fruit with a high nutritional value; however, little information is available regarding the comprehensive utilization of its byproducts.					
37178889	0	0	theme	vera	91:94	arg1	gel					96:98	Aloe vera gel	86:98	Aloe vera gel	86:98	Chitosan-based composite films containing eugenol nanoemulsion, ZnO nanoparticles and Aloe vera gel for active food packaging.					
37178889	0	1	theme	Aloe	86:89	arg1	gel					96:98	Aloe vera gel	86:98	Aloe vera gel	86:98	Chitosan-based composite films containing eugenol nanoemulsion, ZnO nanoparticles and Aloe vera gel for active food packaging.					
37178889	6	2	theme	incorporated	1109:1120	arg1	agents					1129:1134	the incorporated active agents	1105:1134	the incorporated active agents	1105:1134	The XRD spectra of the fabricated films showed good compatibility between the chitosan and the incorporated active agents.					
37178889	3	3	theme	active	711:716	arg1	nature					718:723	active nature	711:723	active nature of the films	711:736	The rate of release of EuNE from the fabricated films was also evaluated to determine active nature of the films.					
37178889	5	4	theme	barrier	909:915	arg1	property					917:924	UV-light barrier property	900:924	UV-light barrier property of the fabricated composite film	900:957	Incorporation of EuNE in chitosan drastically improved UV-light barrier property of the fabricated composite film by 3 to 6 folds, while maintaining their transparency.					
37178889	7	5	theme	tensile	1250:1256	arg1	strength					1258:1265	tensile strength	1250:1265	tensile strength	1250:1265	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	1	6	theme	synthetic	294:302	arg1	packaging					312:320	synthetic plastic packaging	294:320	synthetic plastic packaging	294:320	Biopolymer-based food packaging films are gaining increasing popularity, as consumers' demands for sustainable alternatives and environmental concerns associated with synthetic plastic packaging grow.					
37178889	0	7	theme	food	111:114	arg1	packaging					116:124	active food packaging	104:124	active food packaging	104:124	Chitosan-based composite films containing eugenol nanoemulsion, ZnO nanoparticles and Aloe vera gel for active food packaging.					
37178889	7	8	theme	antibacterial	1194:1206	arg1	properties					1208:1217	their antibacterial properties	1188:1217	their antibacterial properties against foodborne bacteria and tensile strength about 2-folds	1188:1279	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	2	9	theme	vera	443:446	arg1	gel					448:450	Aloe vera gel	438:450	Aloe vera gel	438:450	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	5	10	theme	composite	944:952	arg1	film					954:957	the fabricated composite film	929:957	the fabricated composite film	929:957	Incorporation of EuNE in chitosan drastically improved UV-light barrier property of the fabricated composite film by 3 to 6 folds, while maintaining their transparency.					
37178889	0	11	theme	active	104:109	arg1	packaging					116:124	active food packaging	104:124	active food packaging	104:124	Chitosan-based composite films containing eugenol nanoemulsion, ZnO nanoparticles and Aloe vera gel for active food packaging.					
37178889	7	12	dep	95 	1383:1385	arg1	to					1380:1381	to	1380:1381	to	1380:1381	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	0	13	theme	Chitosan-based	0:13	arg1	films					25:29	Chitosan-based composite films	0:29	Chitosan-based composite films	0:29	Chitosan-based composite films containing eugenol nanoemulsion, ZnO nanoparticles and Aloe vera gel for active food packaging.					
37178889	7	14	theme	foodborne	1227:1235	arg1	bacteria					1237:1244	foodborne bacteria	1227:1244	foodborne bacteria	1227:1244	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	0	15	theme	composite	15:23	arg1	films					25:29	Chitosan-based composite films	0:29	Chitosan-based composite films	0:29	Chitosan-based composite films containing eugenol nanoemulsion, ZnO nanoparticles and Aloe vera gel for active food packaging.					
37178889	3	16	theme	films	732:736	arg1	nature					718:723	active nature	711:723	active nature of the films	711:736	The rate of release of EuNE from the fabricated films was also evaluated to determine active nature of the films.					
37178889	7	17	theme	scavenging	1334:1343	arg1	activities					1345:1354	DPPH scavenging activities	1329:1354	DPPH scavenging activities of the chitosan film up to 95 %	1329:1386	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	4	18	theme	droplet	748:754	arg1	size					756:759	The EuNE droplet size	739:759	The EuNE droplet size	739:759	The EuNE droplet size was about 200 nm, and they were uniformly distributed throughout the film matrices.					
37178889	4	18	theme	droplet	748:754	arg1	200 nm					771:776	200 nm	771:776	200 nm	771:776	The EuNE droplet size was about 200 nm, and they were uniformly distributed throughout the film matrices.					
37178889	7	19	theme	AVG	1316:1318	arg1	incorporation					1290:1302	incorporation	1290:1302	incorporation of EuNE and AVG	1290:1318	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	7	20	theme	chitosan	1363:1370	arg1	film					1372:1375	the chitosan film	1359:1375	the chitosan film up to 95 %	1359:1386	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	2	21	theme	zinc	457:460	arg1	oxide					462:466	zinc oxide	457:466	zinc oxide	457:466	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	1	22	theme	plastic	304:310	arg1	packaging					312:320	synthetic plastic packaging	294:320	synthetic plastic packaging	294:320	Biopolymer-based food packaging films are gaining increasing popularity, as consumers' demands for sustainable alternatives and environmental concerns associated with synthetic plastic packaging grow.					
37178889	7	23	dep	improved	1179:1186	arg1	whereas					1282:1288	whereas	1282:1288	whereas	1282:1288	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	2	24	theme	research	336:343	arg1	work					345:348	this research work	331:348	this research work	331:348	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	5	25	dep	6	967:967	arg1	to					964:965	to	964:965	to	964:965	Incorporation of EuNE in chitosan drastically improved UV-light barrier property of the fabricated composite film by 3 to 6 folds, while maintaining their transparency.					
37178889	5	25	dep	6	967:967	arg1	folds					969:973	folds	969:973	folds	969:973	Incorporation of EuNE in chitosan drastically improved UV-light barrier property of the fabricated composite film by 3 to 6 folds, while maintaining their transparency.					
37178889	2	26	theme	nanoemulsion	417:428	arg1	ZnONPs					483:488	ZnONPs	483:488	ZnONPs	483:488	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	2	26	theme	nanoemulsion	417:428	arg1	nanoparticles					468:480	eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles	409:480	eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs)	409:489	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	5	27	theme	film	954:957	arg1	property					917:924	UV-light barrier property	900:924	UV-light barrier property of the fabricated composite film	900:957	Incorporation of EuNE in chitosan drastically improved UV-light barrier property of the fabricated composite film by 3 to 6 folds, while maintaining their transparency.					
37178889	6	28	theme	films	1048:1052	arg1	spectra					1022:1028	The XRD spectra	1014:1028	The XRD spectra of the fabricated films	1014:1052	The XRD spectra of the fabricated films showed good compatibility between the chitosan and the incorporated active agents.					
37178889	2	29	theme	gel	448:450	arg1	ZnONPs					483:488	ZnONPs	483:488	ZnONPs	483:488	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	2	29	theme	gel	448:450	arg1	nanoparticles					468:480	eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles	409:480	eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs)	409:489	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	4	30	theme	EuNE	743:746	arg1	size					756:759	The EuNE droplet size	739:759	The EuNE droplet size	739:759	The EuNE droplet size was about 200 nm, and they were uniformly distributed throughout the film matrices.					
37178889	4	30	theme	EuNE	743:746	arg1	200 nm					771:776	200 nm	771:776	200 nm	771:776	The EuNE droplet size was about 200 nm, and they were uniformly distributed throughout the film matrices.					
37178889	2	31	theme	optical	563:569	arg1	properties					571:580	optical properties	563:580	optical properties	563:580	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	2	31	theme	optical	563:569	arg1	solubility					535:544	their solubility	529:544	their solubility	529:544	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	3	32	theme	fabricated	662:671	arg1	films					673:677	the fabricated films	658:677	the fabricated films	658:677	The rate of release of EuNE from the fabricated films was also evaluated to determine active nature of the films.					
37178889	6	33	theme	fabricated	1037:1046	arg1	films					1048:1052	the fabricated films	1033:1052	the fabricated films	1033:1052	The XRD spectra of the fabricated films showed good compatibility between the chitosan and the incorporated active agents.					
37178889	1	34	theme	packaging	149:157	arg1	films					159:163	Biopolymer-based food packaging films	127:163	Biopolymer-based food packaging films	127:163	Biopolymer-based food packaging films are gaining increasing popularity, as consumers' demands for sustainable alternatives and environmental concerns associated with synthetic plastic packaging grow.					
37178889	5	35	theme	fabricated	933:942	arg1	film					954:957	the fabricated composite film	929:957	the fabricated composite film	929:957	Incorporation of EuNE in chitosan drastically improved UV-light barrier property of the fabricated composite film by 3 to 6 folds, while maintaining their transparency.					
37178889	1	36	dep	environmental	255:267	arg1	concerns					269:276	concerns	269:276	concerns	269:276	Biopolymer-based food packaging films are gaining increasing popularity, as consumers' demands for sustainable alternatives and environmental concerns associated with synthetic plastic packaging grow.					
37178889	3	37	from	rate	629:632	arg1	films					673:677	the fabricated films	658:677	the fabricated films	658:677	The rate of release of EuNE from the fabricated films was also evaluated to determine active nature of the films.					
37178889	2	38	theme	Aloe	438:441	arg1	gel					448:450	Aloe vera gel	438:450	Aloe vera gel	438:450	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	0	39	theme	eugenol	42:48	arg1	nanoemulsion					50:61	eugenol nanoemulsion	42:61	eugenol nanoemulsion	42:61	Chitosan-based composite films containing eugenol nanoemulsion, ZnO nanoparticles and Aloe vera gel for active food packaging.					
37178889	7	40	dep	film	1372:1375	arg1	%					1386:1386	%	1386:1386	%	1386:1386	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	3	41	from	films	673:677	arg1	release					637:643	release	637:643	release of EuNE from the fabricated films	637:677	The rate of release of EuNE from the fabricated films was also evaluated to determine active nature of the films.					
37178889	3	41	from	films	673:677	arg1	rate					629:632	The rate	625:632	The rate of release of EuNE from the fabricated films	625:677	The rate of release of EuNE from the fabricated films was also evaluated to determine active nature of the films.					
37178889	0	42	theme	ZnO	64:66	arg1	nanoparticles					68:80	ZnO nanoparticles	64:80	ZnO nanoparticles	64:80	Chitosan-based composite films containing eugenol nanoemulsion, ZnO nanoparticles and Aloe vera gel for active food packaging.					
37178889	5	43	theme	UV-light	900:907	arg1	property					917:924	UV-light barrier property	900:924	UV-light barrier property of the fabricated composite film	900:957	Incorporation of EuNE in chitosan drastically improved UV-light barrier property of the fabricated composite film by 3 to 6 folds, while maintaining their transparency.					
37178889	2	44	theme	antioxidant	601:611	arg1	activities					613:622	antimicrobial and antioxidant activities	583:622	activities	613:622	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	2	44	theme	antioxidant	601:611	arg1	solubility					535:544	their solubility	529:544	their solubility	529:544	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	6	45	theme	active	1122:1127	arg1	agents					1129:1134	the incorporated active agents	1105:1134	the incorporated active agents	1105:1134	The XRD spectra of the fabricated films showed good compatibility between the chitosan and the incorporated active agents.					
37178889	2	46	theme	antimicrobial	373:385	arg1	films					387:391	chitosan-based active antimicrobial films	351:391	chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs)	351:489	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	7	47	theme	ZnONPs	1158:1163	arg1	incorporation					1141:1153	The incorporation	1137:1153	The incorporation of ZnONPs	1137:1163	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	7	48	theme	film	1372:1375	arg1	activities					1345:1354	DPPH scavenging activities	1329:1354	DPPH scavenging activities of the chitosan film up to 95 %	1329:1386	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	4	49	theme	film	830:833	arg1	matrices					835:842	the film matrices	826:842	the film matrices	826:842	The EuNE droplet size was about 200 nm, and they were uniformly distributed throughout the film matrices.					
37178889	2	50	theme	active	366:371	arg1	films					387:391	chitosan-based active antimicrobial films	351:391	chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs)	351:489	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	7	51	theme	EuNE	1307:1310	arg1	incorporation					1290:1302	incorporation	1290:1302	incorporation of EuNE and AVG	1290:1318	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	2	52	theme	antimicrobial	583:595	arg1	activities					613:622	antimicrobial and antioxidant activities	583:622	activities	613:622	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	2	52	theme	antimicrobial	583:595	arg1	solubility					535:544	their solubility	529:544	their solubility	529:544	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	5	53	from	Incorporation	845:857	arg1	chitosan					870:877	chitosan	870:877	chitosan	870:877	Incorporation of EuNE in chitosan drastically improved UV-light barrier property of the fabricated composite film by 3 to 6 folds, while maintaining their transparency.					
37178889	1	54	theme	Biopolymer-based	127:142	arg1	films					159:163	Biopolymer-based food packaging films	127:163	Biopolymer-based food packaging films	127:163	Biopolymer-based food packaging films are gaining increasing popularity, as consumers' demands for sustainable alternatives and environmental concerns associated with synthetic plastic packaging grow.					
37178889	1	55	theme	sustainable	226:236	arg1	alternatives					238:249	sustainable alternatives	226:249	sustainable alternatives	226:249	Biopolymer-based food packaging films are gaining increasing popularity, as consumers' demands for sustainable alternatives and environmental concerns associated with synthetic plastic packaging grow.					
37178889	7	56	theme	DPPH	1329:1332	arg1	activities					1345:1354	DPPH scavenging activities	1329:1354	DPPH scavenging activities of the chitosan film up to 95 %	1329:1386	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	3	57	theme	EuNE	648:651	arg1	release					637:643	release	637:643	release of EuNE from the fabricated films	637:677	The rate of release of EuNE from the fabricated films was also evaluated to determine active nature of the films.					
37178889	7	58	dep	%	1386:1386	arg1	95 					1383:1385	95 	1383:1385	95 	1383:1385	The incorporation of ZnONPs significantly improved their antibacterial properties against foodborne bacteria and tensile strength about 2-folds, whereas incorporation of EuNE and AVG improved DPPH scavenging activities of the chitosan film up to 95 %, respectively.					
37178889	1	59	theme	food	144:147	arg1	films					159:163	Biopolymer-based food packaging films	127:163	Biopolymer-based food packaging films	127:163	Biopolymer-based food packaging films are gaining increasing popularity, as consumers' demands for sustainable alternatives and environmental concerns associated with synthetic plastic packaging grow.					
37178889	2	60	theme	chitosan-based	351:364	arg1	films					387:391	chitosan-based active antimicrobial films	351:391	chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs)	351:489	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	5	61	theme	EuNE	862:865	arg1	Incorporation					845:857	Incorporation	845:857	Incorporation of EuNE in chitosan	845:877	Incorporation of EuNE in chitosan drastically improved UV-light barrier property of the fabricated composite film by 3 to 6 folds, while maintaining their transparency.					
37178889	6	62	theme	good	1061:1064	arg1	compatibility					1066:1078	good compatibility	1061:1078	good compatibility between the chitosan and the incorporated active agents	1061:1134	The XRD spectra of the fabricated films showed good compatibility between the chitosan and the incorporated active agents.					
37178889	2	63	theme	eugenol	409:415	arg1	EuNE					431:434	EuNE	431:434	EuNE	431:434	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	2	63	theme	eugenol	409:415	arg1	nanoemulsion					417:428	eugenol nanoemulsion	409:428	eugenol nanoemulsion (EuNE)	409:435	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	3	64	theme	release	637:643	arg1	rate					629:632	The rate	625:632	The rate of release of EuNE from the fabricated films	625:677	The rate of release of EuNE from the fabricated films was also evaluated to determine active nature of the films.					
37178889	2	65	theme	oxide	462:466	arg1	ZnONPs					483:488	ZnONPs	483:488	ZnONPs	483:488	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	2	65	theme	oxide	462:466	arg1	nanoparticles					468:480	eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles	409:480	eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs)	409:489	In this research work, chitosan-based active antimicrobial films reinforced with eugenol nanoemulsion (EuNE), Aloe vera gel, and zinc oxide nanoparticles (ZnONPs) were fabricated and characterized for their solubility, microstructure, optical properties, antimicrobial and antioxidant activities.					
37178889	6	66	theme	XRD	1018:1020	arg1	spectra					1022:1028	The XRD spectra	1014:1028	The XRD spectra of the fabricated films	1014:1052	The XRD spectra of the fabricated films showed good compatibility between the chitosan and the incorporated active agents.					
37348810	4	0	theme	no	889:890	arg1	ethanol					892:898	almost no ethanol	882:898	almost no ethanol	882:898	The final engineered strain produced 52.8 g/L ethanol directly from cellulose, and 39.8 g/L from corncob, without the need for any added cellulase, while the starting strain produced almost no ethanol.					
37348810	4	1	theme	g/L	741:743	arg1	ethanol					745:751	52.8 g/L ethanol	736:751	52.8 g/L ethanol	736:751	The final engineered strain produced 52.8 g/L ethanol directly from cellulose, and 39.8 g/L from corncob, without the need for any added cellulase, while the starting strain produced almost no ethanol.					
37348810	0	2	theme	Myceliophthora	101:114	arg1	thermophila					116:126	metabolically engineered cellulolytic fungus Myceliophthora thermophila	56:126	metabolically engineered cellulolytic fungus Myceliophthora thermophila	56:126	Consolidated bioprocessing for bioethanol production by metabolically engineered cellulolytic fungus Myceliophthora thermophila.					
37348810	4	3	theme	52.8	736:739	arg1	g/L					741:743	g/L	741:743	g/L	741:743	The final engineered strain produced 52.8 g/L ethanol directly from cellulose, and 39.8 g/L from corncob, without the need for any added cellulase, while the starting strain produced almost no ethanol.					
37348810	5	4	theme	by	954:955	arg1	increases					983:991	the ethanol fermentation by engineered M. thermophila increases	929:991	the ethanol fermentation by engineered M. thermophila increases	929:991	We also demonstrate that as the ethanol fermentation by engineered M. thermophila increases, the composition and expression of cellulases that facilitate the degradation of cellulose, especially cellobiohydrolases, changes.					
37348810	3	5	theme	cellulosic	372:381	arg1	thermophila					405:415	the thermophilic cellulosic fungus Myceliophthora thermophila	355:415	the thermophilic cellulosic fungus Myceliophthora thermophila	355:415	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	6	6	theme	fungal	1219:1224	arg1	promising					1330:1338	promising	1330:1338	promising	1330:1338	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	6	6	theme	fungal	1219:1224	arg1	technology					1253:1262	the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production)	1215:1325	the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production)	1215:1325	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	3	7	theme	fungus	383:388	arg1	thermophila					405:415	the thermophilic cellulosic fungus Myceliophthora thermophila	355:415	the thermophilic cellulosic fungus Myceliophthora thermophila	355:415	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	4	8	from	corncob	796:802	arg1	g/L					787:789	39.8 g/L	782:789	39.8 g/L from corncob	782:802	The final engineered strain produced 52.8 g/L ethanol directly from cellulose, and 39.8 g/L from corncob, without the need for any added cellulase, while the starting strain produced almost no ethanol.					
37348810	4	8	from	corncob	796:802	arg1	cellulose					767:775	cellulose	767:775	cellulose	767:775	The final engineered strain produced 52.8 g/L ethanol directly from cellulose, and 39.8 g/L from corncob, without the need for any added cellulase, while the starting strain produced almost no ethanol.					
37348810	6	9	theme	bioprocessing	1239:1251	arg1	promising					1330:1338	promising	1330:1338	promising	1330:1338	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	6	9	theme	bioprocessing	1239:1251	arg1	technology					1253:1262	the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production)	1215:1325	the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production)	1215:1325	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	6	10	dep	develop	1272:1278	arg1	one-step					1286:1293	one-step	1286:1293	one-step	1286:1293	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	6	10	dep	develop	1272:1278	arg1	production					1315:1324	one-strain ethanol production	1296:1324	one-strain ethanol production	1296:1324	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	6	11	theme	ethanol	1187:1193	arg1	yield					1195:1199	significantly increased ethanol yield	1163:1199	significantly increased ethanol yield	1163:1199	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	4	12	theme	starting	857:864	arg1	strain					866:871	the starting strain	853:871	the starting strain	853:871	The final engineered strain produced 52.8 g/L ethanol directly from cellulose, and 39.8 g/L from corncob, without the need for any added cellulase, while the starting strain produced almost no ethanol.					
37348810	6	13	theme	simplified	1129:1138	arg1	process					1151:1157	The simplified production process	1125:1157	The simplified production process	1125:1157	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	6	14	theme	consolidated	1226:1237	arg1	promising					1330:1338	promising	1330:1338	promising	1330:1338	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	6	14	theme	consolidated	1226:1237	arg1	technology					1253:1262	the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production)	1215:1325	the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production)	1215:1325	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	3	15	theme	thermophilic	359:370	arg1	thermophila					405:415	the thermophilic cellulosic fungus Myceliophthora thermophila	355:415	the thermophilic cellulosic fungus Myceliophthora thermophila	355:415	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	3	16	theme	synthetic	563:571	arg1	pathway					573:579	the synthetic pathway	559:579	the synthetic pathway	559:579	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	3	17	theme	aerobic	477:483	arg1	fermentation					493:504	the aerobic ethanol fermentation	473:504	the aerobic ethanol fermentation of yeast (the Crabtree effect)	473:535	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	6	18	theme	sustainable	1352:1362	arg1	biomanufacturing					1379:1394	sustainable carbon-neutral biomanufacturing	1352:1394	sustainable carbon-neutral biomanufacturing in the future	1352:1408	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	3	19	theme	glycolytic	596:605	arg1	rate					607:610	the glycolytic rate	592:610	the glycolytic rate	592:610	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	4	20	theme	engineered	709:718	arg1	strain					720:725	The final engineered strain	699:725	The final engineered strain	699:725	The final engineered strain produced 52.8 g/L ethanol directly from cellulose, and 39.8 g/L from corncob, without the need for any added cellulase, while the starting strain produced almost no ethanol.					
37348810	6	21	theme	ethanol	1307:1313	arg1	one-step					1286:1293	one-step	1286:1293	one-step	1286:1293	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	6	21	theme	ethanol	1307:1313	arg1	production					1315:1324	one-strain ethanol production	1296:1324	one-strain ethanol production	1296:1324	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	2	22	theme	degradation	323:333	arg1	cost					305:308	the cost	301:308	the cost of cellulose degradation	301:333	However, the economics of the present technology are unfavorable, especially the cost of cellulose degradation.					
37348810	1	23	theme	decarbonization	201:215	arg1	goals					217:221	the world's decarbonization goals	189:221	the world's decarbonization goals	189:221	Using cellulosic ethanol as fuel is one way to help achieve the world's decarbonization goals.					
37348810	3	24	theme	Myceliophthora	390:403	arg1	thermophila					405:415	the thermophilic cellulosic fungus Myceliophthora thermophila	355:415	the thermophilic cellulosic fungus Myceliophthora thermophila	355:415	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	2	25	theme	cellulose	313:321	arg1	degradation					323:333	cellulose degradation	313:333	cellulose degradation	313:333	However, the economics of the present technology are unfavorable, especially the cost of cellulose degradation.					
37348810	6	26	theme	one-strain	1296:1305	arg1	one-step					1286:1293	one-step	1286:1293	one-step	1286:1293	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	6	26	theme	one-strain	1296:1305	arg1	production					1315:1324	one-strain ethanol production	1296:1324	one-strain ethanol production	1296:1324	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	5	27	theme	engineered	957:966	arg1	increases					983:991	the ethanol fermentation by engineered M. thermophila increases	929:991	the ethanol fermentation by engineered M. thermophila increases	929:991	We also demonstrate that as the ethanol fermentation by engineered M. thermophila increases, the composition and expression of cellulases that facilitate the degradation of cellulose, especially cellobiohydrolases, changes.					
37348810	3	28	theme	consumption	678:688	arg1	pathway					690:696	ethanol consumption pathway	670:696	ethanol consumption pathway	670:696	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	5	29	theme	thermophila	971:981	arg1	increases					983:991	the ethanol fermentation by engineered M. thermophila increases	929:991	the ethanol fermentation by engineered M. thermophila increases	929:991	We also demonstrate that as the ethanol fermentation by engineered M. thermophila increases, the composition and expression of cellulases that facilitate the degradation of cellulose, especially cellobiohydrolases, changes.					
37348810	3	30	theme	yeast	509:513	arg1	fermentation					493:504	the aerobic ethanol fermentation	473:504	the aerobic ethanol fermentation of yeast (the Crabtree effect)	473:535	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	5	31	dep	composition	998:1008	arg1	the					994:996	the	994:996	the	994:996	We also demonstrate that as the ethanol fermentation by engineered M. thermophila increases, the composition and expression of cellulases that facilitate the degradation of cellulose, especially cellobiohydrolases, changes.					
37348810	6	32	theme	increased	1177:1185	arg1	yield					1195:1199	significantly increased ethanol yield	1163:1199	significantly increased ethanol yield	1163:1199	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	3	33	theme	mitochondrial	624:636	arg1	NADH					638:641	mitochondrial NADH shuttles	624:650	mitochondrial NADH shuttles	624:650	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	3	34	dep	NADH	638:641	arg1	shuttles					643:650	shuttles	643:650	shuttles	643:650	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	3	35	theme	ethanol	485:491	arg1	fermentation					493:504	the aerobic ethanol fermentation	473:504	the aerobic ethanol fermentation of yeast (the Crabtree effect)	473:535	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	6	36	from	biomanufacturing	1379:1394	arg1	future					1403:1408	future	1403:1408	future	1403:1408	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	0	37	theme	bioethanol	31:40	arg1	production					42:51	bioethanol production	31:51	bioethanol production by metabolically engineered cellulolytic fungus Myceliophthora thermophila	31:126	Consolidated bioprocessing for bioethanol production by metabolically engineered cellulolytic fungus Myceliophthora thermophila.					
37348810	0	38	theme	engineered	70:79	arg1	thermophila					116:126	metabolically engineered cellulolytic fungus Myceliophthora thermophila	56:126	metabolically engineered cellulolytic fungus Myceliophthora thermophila	56:126	Consolidated bioprocessing for bioethanol production by metabolically engineered cellulolytic fungus Myceliophthora thermophila.					
37348810	3	39	theme	ethanol	670:676	arg1	consumption					678:688	ethanol consumption	670:688	ethanol consumption pathway	670:696	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	3	40	theme	Crabtree	520:527	arg1	effect					529:534	the Crabtree effect	516:534	the aerobic ethanol fermentation of yeast (the Crabtree effect)	473:535	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	5	41	theme	M.	968:969	arg1	increases					983:991	the ethanol fermentation by engineered M. thermophila increases	929:991	the ethanol fermentation by engineered M. thermophila increases	929:991	We also demonstrate that as the ethanol fermentation by engineered M. thermophila increases, the composition and expression of cellulases that facilitate the degradation of cellulose, especially cellobiohydrolases, changes.					
37348810	4	42	theme	final	703:707	arg1	strain					720:725	The final engineered strain	699:725	The final engineered strain	699:725	The final engineered strain produced 52.8 g/L ethanol directly from cellulose, and 39.8 g/L from corncob, without the need for any added cellulase, while the starting strain produced almost no ethanol.					
37348810	5	43	theme	cellulases	1028:1037	arg1	expression					1014:1023	expression	1014:1023	expression	1014:1023	We also demonstrate that as the ethanol fermentation by engineered M. thermophila increases, the composition and expression of cellulases that facilitate the degradation of cellulose, especially cellobiohydrolases, changes.					
37348810	5	43	theme	cellulases	1028:1037	arg1	composition					998:1008	composition	998:1008	composition	998:1008	We also demonstrate that as the ethanol fermentation by engineered M. thermophila increases, the composition and expression of cellulases that facilitate the degradation of cellulose, especially cellobiohydrolases, changes.					
37348810	5	44	theme	cellulose	1074:1082	arg1	degradation					1059:1069	the degradation	1055:1069	the degradation of cellulose	1055:1082	We also demonstrate that as the ethanol fermentation by engineered M. thermophila increases, the composition and expression of cellulases that facilitate the degradation of cellulose, especially cellobiohydrolases, changes.					
37348810	4	45	theme	added	830:834	arg1	cellulase					836:844	any added cellulase	826:844	any added cellulase	826:844	The final engineered strain produced 52.8 g/L ethanol directly from cellulose, and 39.8 g/L from corncob, without the need for any added cellulase, while the starting strain produced almost no ethanol.					
37348810	5	46	theme	ethanol	933:939	arg1	increases					983:991	the ethanol fermentation by engineered M. thermophila increases	929:991	the ethanol fermentation by engineered M. thermophila increases	929:991	We also demonstrate that as the ethanol fermentation by engineered M. thermophila increases, the composition and expression of cellulases that facilitate the degradation of cellulose, especially cellobiohydrolases, changes.					
37348810	1	47	theme	cellulosic	135:144	arg1	ethanol					146:152	cellulosic ethanol	135:152	cellulosic ethanol	135:152	Using cellulosic ethanol as fuel is one way to help achieve the world's decarbonization goals.					
37348810	6	48	theme	carbon-neutral	1364:1377	arg1	biomanufacturing					1379:1394	sustainable carbon-neutral biomanufacturing	1352:1394	sustainable carbon-neutral biomanufacturing in the future	1352:1408	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
37348810	3	49	dep	fermentation	493:504	arg1	effect					529:534	the Crabtree effect	516:534	the aerobic ethanol fermentation of yeast (the Crabtree effect)	473:535	Here, we reprogram the thermophilic cellulosic fungus Myceliophthora thermophila to directly ferment cellulose into ethanol by mimicking the aerobic ethanol fermentation of yeast (the Crabtree effect), including optimizing the synthetic pathway, enhancing the glycolytic rate, inhibiting mitochondrial NADH shuttles, and knocking out ethanol consumption pathway.					
37348810	5	50	theme	fermentation	941:952	arg1	increases					983:991	the ethanol fermentation by engineered M. thermophila increases	929:991	the ethanol fermentation by engineered M. thermophila increases	929:991	We also demonstrate that as the ethanol fermentation by engineered M. thermophila increases, the composition and expression of cellulases that facilitate the degradation of cellulose, especially cellobiohydrolases, changes.					
37348810	0	51	theme	fungus	94:99	arg1	thermophila					116:126	metabolically engineered cellulolytic fungus Myceliophthora thermophila	56:126	metabolically engineered cellulolytic fungus Myceliophthora thermophila	56:126	Consolidated bioprocessing for bioethanol production by metabolically engineered cellulolytic fungus Myceliophthora thermophila.					
37348810	2	52	theme	technology	262:271	arg1	economics					237:245	the economics	233:245	the economics of the present technology	233:271	However, the economics of the present technology are unfavorable, especially the cost of cellulose degradation.					
37348810	2	52	theme	technology	262:271	arg1	unfavorable					277:287	unfavorable	277:287	unfavorable	277:287	However, the economics of the present technology are unfavorable, especially the cost of cellulose degradation.					
37348810	0	53	theme	cellulolytic	81:92	arg1	thermophila					116:126	metabolically engineered cellulolytic fungus Myceliophthora thermophila	56:126	metabolically engineered cellulolytic fungus Myceliophthora thermophila	56:126	Consolidated bioprocessing for bioethanol production by metabolically engineered cellulolytic fungus Myceliophthora thermophila.					
37348810	2	54	theme	present	254:260	arg1	technology					262:271	the present technology	250:271	the present technology	250:271	However, the economics of the present technology are unfavorable, especially the cost of cellulose degradation.					
37348810	6	55	theme	production	1140:1149	arg1	process					1151:1157	The simplified production process	1125:1157	The simplified production process	1125:1157	The simplified production process and significantly increased ethanol yield indicate that the fungal consolidated bioprocessing technology that we develop here (one-step, one-strain ethanol production) is promising for fueling sustainable carbon-neutral biomanufacturing in the future.					
36241314	6	0	theme	simple	931:936	arg1	variation					952:960	a simple morphological variation	929:960	a simple morphological variation	929:960	Uniquely, a simple morphological variation can improve the mechanical strengths of BC/PVA cryogels, and this process may be broadly applicable as a tool for biomaterial design and fabrication.					
36241314	3	1	theme	crystalline	509:519	arg1	structure					521:529	a crystalline structure	507:529	a crystalline structure around the BC nanofibers	507:554	Here, we adopt the freeze-thaw process with a water-soluble polymer to form a crystalline structure around the BC nanofibers.					
36241314	4	2	theme	chains	588:593	arg1	crystallization					561:575	The crystallization	557:575	The crystallization of polymer chains such as poly(vinyl alcohol) (PVA)	557:627	The crystallization of polymer chains such as poly(vinyl alcohol) (PVA) occurs spontaneously during this process, which results in the unique and environment-friendly methodology of the composition.					
36241314	1	3	theme	BC	175:176	arg1	structures					179:188	networked bacterial cellulose (BC) structures	144:188	networked bacterial cellulose (BC) structures	144:188	The free-form fabrication of networked bacterial cellulose (BC) structures was available using a particle matrix and a bioink embedding bacteria.					
36241314	0	4	theme	freeze-thaw	94:104	arg1	process					106:112	freeze-thaw process	94:112	freeze-thaw process	94:112	Free-form three-dimensional nanocellulose structure reinforced with poly(vinyl alcohol) using freeze-thaw process.					
36241314	6	5	theme	cryogels	1009:1016	arg1	strengths					989:997	the mechanical strengths	974:997	the mechanical strengths of BC/PVA cryogels	974:1016	Uniquely, a simple morphological variation can improve the mechanical strengths of BC/PVA cryogels, and this process may be broadly applicable as a tool for biomaterial design and fabrication.					
36241314	4	6	theme	unique	692:697	arg1	methodology					724:734	the unique and environment-friendly methodology	688:734	the unique and environment-friendly methodology of the composition	688:753	The crystallization of polymer chains such as poly(vinyl alcohol) (PVA) occurs spontaneously during this process, which results in the unique and environment-friendly methodology of the composition.					
36241314	5	7	theme	BC	818:819	arg1	hydrogels					821:829	free-form BC hydrogels	808:829	free-form BC hydrogels	808:829	Crystallization enhances the mechanical strength of free-form BC hydrogels by filling the space between the nanofiber networks with crystalline polymeric fillers.					
36241314	2	8	theme	mechanical	274:283	arg1	strength					285:292	the mechanical strength	270:292	the mechanical strength of such BC hydrogel structures	270:323	However, the mechanical strength of such BC hydrogel structures does not satisfy the requirements for biomedical applications that require high toughness and elasticity.					
36241314	5	9	theme	nanofiber	864:872	arg1	networks					874:881	the nanofiber networks	860:881	the nanofiber networks with crystalline polymeric fillers	860:916	Crystallization enhances the mechanical strength of free-form BC hydrogels by filling the space between the nanofiber networks with crystalline polymeric fillers.					
36241314	3	10	theme	BC	542:543	arg1	nanofibers					545:554	the BC nanofibers	538:554	the BC nanofibers	538:554	Here, we adopt the freeze-thaw process with a water-soluble polymer to form a crystalline structure around the BC nanofibers.					
36241314	1	11	theme	structures	179:188	arg1	available					194:202	available	194:202	available	194:202	The free-form fabrication of networked bacterial cellulose (BC) structures was available using a particle matrix and a bioink embedding bacteria.					
36241314	1	11	theme	structures	179:188	arg1	fabrication					129:139	The free-form fabrication	115:139	The free-form fabrication of networked bacterial cellulose (BC) structures	115:188	The free-form fabrication of networked bacterial cellulose (BC) structures was available using a particle matrix and a bioink embedding bacteria.					
36241314	0	12	theme	three-dimensional	10:26	arg1	structure					42:50	Free-form three-dimensional nanocellulose structure	0:50	Free-form three-dimensional nanocellulose structure	0:50	Free-form three-dimensional nanocellulose structure reinforced with poly(vinyl alcohol) using freeze-thaw process.					
36241314	2	13	theme	structures	314:323	arg1	strength					285:292	the mechanical strength	270:292	the mechanical strength of such BC hydrogel structures	270:323	However, the mechanical strength of such BC hydrogel structures does not satisfy the requirements for biomedical applications that require high toughness and elasticity.					
36241314	6	14	theme	biomaterial	1076:1086	arg1	design					1088:1093	biomaterial design	1076:1093	biomaterial design	1076:1093	Uniquely, a simple morphological variation can improve the mechanical strengths of BC/PVA cryogels, and this process may be broadly applicable as a tool for biomaterial design and fabrication.					
36241314	0	15	theme	Free-form	0:8	arg1	structure					42:50	Free-form three-dimensional nanocellulose structure	0:50	Free-form three-dimensional nanocellulose structure	0:50	Free-form three-dimensional nanocellulose structure reinforced with poly(vinyl alcohol) using freeze-thaw process.					
36241314	6	16	theme	BC/PVA	1002:1007	arg1	cryogels					1009:1016	BC/PVA cryogels	1002:1016	BC/PVA cryogels	1002:1016	Uniquely, a simple morphological variation can improve the mechanical strengths of BC/PVA cryogels, and this process may be broadly applicable as a tool for biomaterial design and fabrication.					
36241314	2	17	theme	hydrogel	305:312	arg1	structures					314:323	such BC hydrogel structures	297:323	such BC hydrogel structures	297:323	However, the mechanical strength of such BC hydrogel structures does not satisfy the requirements for biomedical applications that require high toughness and elasticity.					
36241314	5	18	theme	crystalline	888:898	arg1	fillers					910:916	crystalline polymeric fillers	888:916	crystalline polymeric fillers	888:916	Crystallization enhances the mechanical strength of free-form BC hydrogels by filling the space between the nanofiber networks with crystalline polymeric fillers.					
36241314	5	19	theme	hydrogels	821:829	arg1	strength					796:803	the mechanical strength	781:803	the mechanical strength of free-form BC hydrogels	781:829	Crystallization enhances the mechanical strength of free-form BC hydrogels by filling the space between the nanofiber networks with crystalline polymeric fillers.					
36241314	2	20	theme	BC	302:303	arg1	structures					314:323	such BC hydrogel structures	297:323	such BC hydrogel structures	297:323	However, the mechanical strength of such BC hydrogel structures does not satisfy the requirements for biomedical applications that require high toughness and elasticity.					
36241314	0	21	theme	nanocellulose	28:40	arg1	structure					42:50	Free-form three-dimensional nanocellulose structure	0:50	Free-form three-dimensional nanocellulose structure	0:50	Free-form three-dimensional nanocellulose structure reinforced with poly(vinyl alcohol) using freeze-thaw process.					
36241314	4	22	theme	polymer	580:586	arg1	chains					588:593	polymer chains	580:593	polymer chains such as poly(vinyl alcohol) (PVA)	580:627	The crystallization of polymer chains such as poly(vinyl alcohol) (PVA) occurs spontaneously during this process, which results in the unique and environment-friendly methodology of the composition.					
36241314	4	22	theme	polymer	580:586	arg1	poly					603:606	poly	603:606	poly(vinyl alcohol) (PVA)	603:627	The crystallization of polymer chains such as poly(vinyl alcohol) (PVA) occurs spontaneously during this process, which results in the unique and environment-friendly methodology of the composition.					
36241314	2	23	theme	such	297:300	arg1	structures					314:323	such BC hydrogel structures	297:323	such BC hydrogel structures	297:323	However, the mechanical strength of such BC hydrogel structures does not satisfy the requirements for biomedical applications that require high toughness and elasticity.					
36241314	5	24	theme	polymeric	900:908	arg1	fillers					910:916	crystalline polymeric fillers	888:916	crystalline polymeric fillers	888:916	Crystallization enhances the mechanical strength of free-form BC hydrogels by filling the space between the nanofiber networks with crystalline polymeric fillers.					
36241314	5	25	theme	mechanical	785:794	arg1	strength					796:803	the mechanical strength	781:803	the mechanical strength of free-form BC hydrogels	781:829	Crystallization enhances the mechanical strength of free-form BC hydrogels by filling the space between the nanofiber networks with crystalline polymeric fillers.					
36241314	6	26	theme	mechanical	978:987	arg1	strengths					989:997	the mechanical strengths	974:997	the mechanical strengths of BC/PVA cryogels	974:1016	Uniquely, a simple morphological variation can improve the mechanical strengths of BC/PVA cryogels, and this process may be broadly applicable as a tool for biomaterial design and fabrication.					
36241314	3	27	theme	freeze-thaw	450:460	arg1	process					462:468	the freeze-thaw process	446:468	the freeze-thaw process	446:468	Here, we adopt the freeze-thaw process with a water-soluble polymer to form a crystalline structure around the BC nanofibers.					
36241314	1	28	theme	particle	212:219	arg1	matrix					221:226	a particle matrix	210:226	a particle matrix	210:226	The free-form fabrication of networked bacterial cellulose (BC) structures was available using a particle matrix and a bioink embedding bacteria.					
36241314	1	29	theme	free-form	119:127	arg1	available					194:202	available	194:202	available	194:202	The free-form fabrication of networked bacterial cellulose (BC) structures was available using a particle matrix and a bioink embedding bacteria.					
36241314	1	29	theme	free-form	119:127	arg1	fabrication					129:139	The free-form fabrication	115:139	The free-form fabrication of networked bacterial cellulose (BC) structures	115:188	The free-form fabrication of networked bacterial cellulose (BC) structures was available using a particle matrix and a bioink embedding bacteria.					
36241314	5	30	theme	free-form	808:816	arg1	hydrogels					821:829	free-form BC hydrogels	808:829	free-form BC hydrogels	808:829	Crystallization enhances the mechanical strength of free-form BC hydrogels by filling the space between the nanofiber networks with crystalline polymeric fillers.					
36241314	1	31	theme	cellulose	164:172	arg1	structures					179:188	networked bacterial cellulose (BC) structures	144:188	networked bacterial cellulose (BC) structures	144:188	The free-form fabrication of networked bacterial cellulose (BC) structures was available using a particle matrix and a bioink embedding bacteria.					
36241314	3	32	theme	water-soluble	477:489	arg1	polymer					491:497	a water-soluble polymer	475:497	a water-soluble polymer to form a crystalline structure around the BC nanofibers	475:554	Here, we adopt the freeze-thaw process with a water-soluble polymer to form a crystalline structure around the BC nanofibers.					
36241314	4	33	theme	composition	743:753	arg1	methodology					724:734	the unique and environment-friendly methodology	688:734	the unique and environment-friendly methodology of the composition	688:753	The crystallization of polymer chains such as poly(vinyl alcohol) (PVA) occurs spontaneously during this process, which results in the unique and environment-friendly methodology of the composition.					
36241314	4	34	theme	vinyl	608:612	arg1	poly					603:606	poly	603:606	poly(vinyl alcohol) (PVA)	603:627	The crystallization of polymer chains such as poly(vinyl alcohol) (PVA) occurs spontaneously during this process, which results in the unique and environment-friendly methodology of the composition.					
36241314	4	34	theme	vinyl	608:612	arg1	alcohol					614:620	vinyl alcohol	608:620	vinyl alcohol	608:620	The crystallization of polymer chains such as poly(vinyl alcohol) (PVA) occurs spontaneously during this process, which results in the unique and environment-friendly methodology of the composition.					
36241314	5	35	with	networks	874:881	arg1	fillers					910:916	crystalline polymeric fillers	888:916	crystalline polymeric fillers	888:916	Crystallization enhances the mechanical strength of free-form BC hydrogels by filling the space between the nanofiber networks with crystalline polymeric fillers.					
36241314	1	36	theme	networked	144:152	arg1	structures					179:188	networked bacterial cellulose (BC) structures	144:188	networked bacterial cellulose (BC) structures	144:188	The free-form fabrication of networked bacterial cellulose (BC) structures was available using a particle matrix and a bioink embedding bacteria.					
36241314	4	37	theme	environment-friendly	703:722	arg1	methodology					724:734	the unique and environment-friendly methodology	688:734	the unique and environment-friendly methodology of the composition	688:753	The crystallization of polymer chains such as poly(vinyl alcohol) (PVA) occurs spontaneously during this process, which results in the unique and environment-friendly methodology of the composition.					
36241314	1	38	theme	bacterial	154:162	arg1	structures					179:188	networked bacterial cellulose (BC) structures	144:188	networked bacterial cellulose (BC) structures	144:188	The free-form fabrication of networked bacterial cellulose (BC) structures was available using a particle matrix and a bioink embedding bacteria.					
36241314	2	39	theme	high	400:403	arg1	toughness					405:413	high toughness	400:413	high toughness	400:413	However, the mechanical strength of such BC hydrogel structures does not satisfy the requirements for biomedical applications that require high toughness and elasticity.					
36241314	0	40	theme	vinyl	73:77	arg1	poly					68:71	poly	68:71	poly(vinyl alcohol) using freeze-thaw process	68:112	Free-form three-dimensional nanocellulose structure reinforced with poly(vinyl alcohol) using freeze-thaw process.					
36241314	0	40	theme	vinyl	73:77	arg1	alcohol					79:85	vinyl alcohol	73:85	vinyl alcohol	73:85	Free-form three-dimensional nanocellulose structure reinforced with poly(vinyl alcohol) using freeze-thaw process.					
36241314	6	41	theme	morphological	938:950	arg1	variation					952:960	a simple morphological variation	929:960	a simple morphological variation	929:960	Uniquely, a simple morphological variation can improve the mechanical strengths of BC/PVA cryogels, and this process may be broadly applicable as a tool for biomaterial design and fabrication.					
36241314	2	42	theme	biomedical	363:372	arg1	applications					374:385	biomedical applications	363:385	biomedical applications that require high toughness and elasticity	363:428	However, the mechanical strength of such BC hydrogel structures does not satisfy the requirements for biomedical applications that require high toughness and elasticity.					
35220078	2	0	theme	film	364:367	arg1	treatment					336:344	a laser treatment	328:344	a laser treatment of polyimide (PI) film coated with CS solution containing gold ions (Au (III))	328:423	The fabrication was based on a laser treatment of polyimide (PI) film coated with CS solution containing gold ions (Au (III)).					
35220078	1	1	theme	LIG-CS-AuNPs	274:285	arg1	electrode					288:296	a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	211:296	a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	211:296	We developed a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode.					
35220078	0	2	theme	graphene	82:89	arg1	electrode					91:99	gold nanoparticle/chitosan/ porous graphene electrode	47:99	gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection	47:123	Facile fabrication of a flexible laser induced gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection.					
35220078	6	3	theme	DPAdSV	896:901	arg1	parameters					882:891	The affecting parameters	868:891	The affecting parameters of DPAdSV	868:901	The affecting parameters of DPAdSV were optimized.					
35220078	5	4	theme	uric	662:665	arg1	UA					673:674	UA	673:674	UA	673:674	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	5	4	theme	uric	662:665	arg1	acid					667:670	uric acid	662:670	uric acid (UA)	662:675	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	7	5	theme	UA	952:953	arg1	determination					955:967	UA determination	952:967	UA determination	952:967	Under the optimal conditions for UA determination, the limit of detection of the developed sensor was 0.33 μmol L-1 and the limit of quantification 1.10 μmol L-1.					
35220078	9	6	theme	blood	1262:1266	arg1	serum					1268:1272	blood serum	1262:1272	blood serum	1262:1272	The sensor was highly sensitive and demonstrated excellent reproducibility and selectivity, determining UA in blood serum with recoveries between 86.6 ± 0.6 and 94.7 ± 0.4%.					
35220078	4	7	theme	electrocatalytic	576:591	arg1	activity					593:600	excellent electrocatalytic activity	566:600	excellent electrocatalytic activity	566:600	The electrode exhibited good electrical conductivity and excellent electrocatalytic activity toward the oxidation of UA.					
35220078	0	8	theme	uric	105:108	arg1	acid					110:113	uric acid	105:113	uric acid detection	105:123	Facile fabrication of a flexible laser induced gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection.					
35220078	5	9	theme	acid	667:670	arg1	monitoring					648:657	on-site monitoring	640:657	on-site monitoring of uric acid (UA) in blood serum	640:690	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	1	10	theme	electrode	288:296	arg1	fabrication					196:206	a simple, inexpensive, environmentally friendly one-step fabrication	139:206	a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	139:296	We developed a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode.					
35220078	5	11	theme	stripping	725:733	arg1	voltammetry					735:745	differential pulse adsorptive stripping voltammetry	695:745	differential pulse adsorptive stripping voltammetry (DPAdSV)	695:754	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	5	11	theme	stripping	725:733	arg1	DPAdSV					748:753	DPAdSV	748:753	DPAdSV	748:753	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	10	12	theme	enzymatic	1401:1409	arg1	method					1411:1416	the hospital standard enzymatic method	1379:1416	the hospital standard enzymatic method	1379:1416	The analysis results were in agreement with those of the hospital standard enzymatic method.					
35220078	9	13	from	UA	1256:1257	arg1	serum					1268:1272	blood serum	1262:1272	blood serum	1262:1272	The sensor was highly sensitive and demonstrated excellent reproducibility and selectivity, determining UA in blood serum with recoveries between 86.6 ± 0.6 and 94.7 ± 0.4%.					
35220078	3	14	theme	graphene	462:469	arg1	composite					471:479	the induced 3D graphene composite	447:479	the induced 3D graphene composite	447:479	The AuNPs decorating the induced 3D graphene composite were uniformly distributed.					
35220078	9	15	theme	excellent	1201:1209	arg1	reproducibility					1211:1225	excellent reproducibility	1201:1225	excellent reproducibility	1201:1225	The sensor was highly sensitive and demonstrated excellent reproducibility and selectivity, determining UA in blood serum with recoveries between 86.6 ± 0.6 and 94.7 ± 0.4%.					
35220078	2	16	theme	gold	404:407	arg1	Au					415:416	Au (III)	415:422	Au (III)	415:422	The fabrication was based on a laser treatment of polyimide (PI) film coated with CS solution containing gold ions (Au (III)).					
35220078	2	16	theme	gold	404:407	arg1	ions					409:412	gold ions	404:412	gold ions (Au (III))	404:423	The fabrication was based on a laser treatment of polyimide (PI) film coated with CS solution containing gold ions (Au (III)).					
35220078	0	17	theme	acid	110:113	arg1	detection					115:123	uric acid detection	105:123	uric acid detection	105:123	Facile fabrication of a flexible laser induced gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection.					
35220078	5	18	theme	portable	788:795	arg1	potentiostat					797:808	a portable potentiostat	786:808	a portable potentiostat connected to a cellphone to control and monitor analysis	786:865	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	8	19	theme	linear	1086:1091	arg1	ranges					1093:1098	Two linear ranges	1082:1098	Two linear ranges	1082:1098	Two linear ranges were produced: 1.0-30 μmol L-1 and 30-100 μmol L-1.					
35220078	1	20	theme	friendly	178:185	arg1	fabrication					196:206	a simple, inexpensive, environmentally friendly one-step fabrication	139:206	a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	139:296	We developed a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode.					
35220078	5	21	theme	on-site	640:646	arg1	monitoring					648:657	on-site monitoring	640:657	on-site monitoring of uric acid (UA) in blood serum	640:690	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	7	22	theme	optimal	929:935	arg1	conditions					937:946	the optimal conditions	925:946	the optimal conditions for UA determination	925:967	Under the optimal conditions for UA determination, the limit of detection of the developed sensor was 0.33 μmol L-1 and the limit of quantification 1.10 μmol L-1.					
35220078	6	23	theme	affecting	872:880	arg1	parameters					882:891	The affecting parameters	868:891	The affecting parameters of DPAdSV	868:901	The affecting parameters of DPAdSV were optimized.					
35220078	1	24	theme	one-step	187:194	arg1	fabrication					196:206	a simple, inexpensive, environmentally friendly one-step fabrication	139:206	a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	139:296	We developed a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode.					
35220078	0	25	theme	Facile	0:5	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of a flexible laser	0:37	Facile fabrication of a flexible laser induced gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection.					
35220078	2	26	theme	laser	330:334	arg1	treatment					336:344	a laser treatment	328:344	a laser treatment of polyimide (PI) film coated with CS solution containing gold ions (Au (III))	328:423	The fabrication was based on a laser treatment of polyimide (PI) film coated with CS solution containing gold ions (Au (III)).					
35220078	2	27	theme	CS	381:382	arg1	solution					384:391	CS solution	381:391	CS solution containing gold ions (Au (III))	381:423	The fabrication was based on a laser treatment of polyimide (PI) film coated with CS solution containing gold ions (Au (III)).					
35220078	4	28	theme	electrical	538:547	arg1	conductivity					549:560	good electrical conductivity	533:560	good electrical conductivity	533:560	The electrode exhibited good electrical conductivity and excellent electrocatalytic activity toward the oxidation of UA.					
35220078	10	29	theme	standard	1392:1399	arg1	method					1411:1416	the hospital standard enzymatic method	1379:1416	the hospital standard enzymatic method	1379:1416	The analysis results were in agreement with those of the hospital standard enzymatic method.					
35220078	1	30	dep	simple	141:146	arg1	environmentally					162:176	environmentally	162:176	environmentally	162:176	We developed a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode.					
35220078	1	30	dep	simple	141:146	arg1	inexpensive					149:159	inexpensive	149:159	inexpensive	149:159	We developed a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode.					
35220078	10	31	theme	analysis	1330:1337	arg1	results					1339:1345	The analysis results	1326:1345	The analysis results	1326:1345	The analysis results were in agreement with those of the hospital standard enzymatic method.					
35220078	10	32	with	agreement	1355:1363	arg1	those					1370:1374	those	1370:1374	those	1370:1374	The analysis results were in agreement with those of the hospital standard enzymatic method.					
35220078	4	33	theme	excellent	566:574	arg1	activity					593:600	excellent electrocatalytic activity	566:600	excellent electrocatalytic activity	566:600	The electrode exhibited good electrical conductivity and excellent electrocatalytic activity toward the oxidation of UA.					
35220078	7	34	theme	quantification	1052:1065	arg1	1.10 μmol L-1					1067:1079	quantification 1.10 μmol L-1	1052:1079	quantification 1.10 μmol L-1	1052:1079	Under the optimal conditions for UA determination, the limit of detection of the developed sensor was 0.33 μmol L-1 and the limit of quantification 1.10 μmol L-1.					
35220078	0	35	theme	laser	33:37	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of a flexible laser	0:37	Facile fabrication of a flexible laser induced gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection.					
35220078	5	36	theme	differential	695:706	arg1	voltammetry					735:745	differential pulse adsorptive stripping voltammetry	695:745	differential pulse adsorptive stripping voltammetry (DPAdSV)	695:754	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	5	36	theme	differential	695:706	arg1	DPAdSV					748:753	DPAdSV	748:753	DPAdSV	748:753	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	1	37	theme	flexible	213:220	arg1	electrode					288:296	a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	211:296	a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	211:296	We developed a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode.					
35220078	0	38	theme	flexible	24:31	arg1	laser					33:37	a flexible laser	22:37	a flexible laser	22:37	Facile fabrication of a flexible laser induced gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection.					
35220078	5	39	theme	pulse	708:712	arg1	voltammetry					735:745	differential pulse adsorptive stripping voltammetry	695:745	differential pulse adsorptive stripping voltammetry (DPAdSV)	695:754	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	5	39	theme	pulse	708:712	arg1	DPAdSV					748:753	DPAdSV	748:753	DPAdSV	748:753	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	2	40	contain	containing	393:402	arg2	Au					415:416	Au (III)	415:422	Au (III)	415:422	The fabrication was based on a laser treatment of polyimide (PI) film coated with CS solution containing gold ions (Au (III)).					
35220078	2	40	contain	containing	393:402	arg1	solution					384:391	CS solution	381:391	CS solution containing gold ions (Au (III))	381:423	The fabrication was based on a laser treatment of polyimide (PI) film coated with CS solution containing gold ions (Au (III)).					
35220078	2	40	contain	containing	393:402	arg2	ions					409:412	gold ions	404:412	gold ions (Au (III))	404:423	The fabrication was based on a laser treatment of polyimide (PI) film coated with CS solution containing gold ions (Au (III)).					
35220078	1	41	theme	laser	222:226	arg1	electrode					288:296	a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	211:296	a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	211:296	We developed a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode.					
35220078	0	42	theme	gold	47:50	arg1	electrode					91:99	gold nanoparticle/chitosan/ porous graphene electrode	47:99	gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection	47:123	Facile fabrication of a flexible laser induced gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection.					
35220078	5	43	theme	adsorptive	714:723	arg1	voltammetry					735:745	differential pulse adsorptive stripping voltammetry	695:745	differential pulse adsorptive stripping voltammetry (DPAdSV)	695:754	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	5	43	theme	adsorptive	714:723	arg1	DPAdSV					748:753	DPAdSV	748:753	DPAdSV	748:753	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	5	44	from	monitoring	648:657	arg1	serum					686:690	blood serum	680:690	blood serum	680:690	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	7	45	theme	developed	1000:1008	arg1	sensor					1010:1015	the developed sensor	996:1015	the developed sensor	996:1015	Under the optimal conditions for UA determination, the limit of detection of the developed sensor was 0.33 μmol L-1 and the limit of quantification 1.10 μmol L-1.					
35220078	2	46	theme	PI	360:361	arg1	film					364:367	polyimide (PI) film	349:367	polyimide (PI) film coated with CS solution containing gold ions (Au (III))	349:423	The fabrication was based on a laser treatment of polyimide (PI) film coated with CS solution containing gold ions (Au (III)).					
35220078	4	47	theme	good	533:536	arg1	conductivity					549:560	good electrical conductivity	533:560	good electrical conductivity	533:560	The electrode exhibited good electrical conductivity and excellent electrocatalytic activity toward the oxidation of UA.					
35220078	3	48	theme	induced	451:457	arg1	composite					471:479	the induced 3D graphene composite	447:479	the induced 3D graphene composite	447:479	The AuNPs decorating the induced 3D graphene composite were uniformly distributed.					
35220078	7	49	theme	1.10 μmol L-1	1067:1079	arg1	0.33 μmol L-1					1021:1033	0.33 μmol L-1	1021:1033	0.33 μmol L-1	1021:1033	Under the optimal conditions for UA determination, the limit of detection of the developed sensor was 0.33 μmol L-1 and the limit of quantification 1.10 μmol L-1.					
35220078	7	49	theme	1.10 μmol L-1	1067:1079	arg1	limit					1043:1047	the limit	1039:1047	the limit of quantification 1.10 μmol L-1	1039:1079	Under the optimal conditions for UA determination, the limit of detection of the developed sensor was 0.33 μmol L-1 and the limit of quantification 1.10 μmol L-1.					
35220078	7	49	theme	1.10 μmol L-1	1067:1079	arg1	limit					974:978	the limit	970:978	the limit of detection of the developed sensor	970:1015	Under the optimal conditions for UA determination, the limit of detection of the developed sensor was 0.33 μmol L-1 and the limit of quantification 1.10 μmol L-1.					
35220078	7	50	theme	detection	983:991	arg1	limit					974:978	the limit	970:978	the limit of detection of the developed sensor	970:1015	Under the optimal conditions for UA determination, the limit of detection of the developed sensor was 0.33 μmol L-1 and the limit of quantification 1.10 μmol L-1.					
35220078	7	50	theme	detection	983:991	arg1	0.33 μmol L-1					1021:1033	0.33 μmol L-1	1021:1033	0.33 μmol L-1	1021:1033	Under the optimal conditions for UA determination, the limit of detection of the developed sensor was 0.33 μmol L-1 and the limit of quantification 1.10 μmol L-1.					
35220078	7	51	theme	sensor	1010:1015	arg1	detection					983:991	detection	983:991	detection of the developed sensor	983:1015	Under the optimal conditions for UA determination, the limit of detection of the developed sensor was 0.33 μmol L-1 and the limit of quantification 1.10 μmol L-1.					
35220078	2	52	theme	polyimide	349:357	arg1	film					364:367	polyimide (PI) film	349:367	polyimide (PI) film coated with CS solution containing gold ions (Au (III))	349:423	The fabrication was based on a laser treatment of polyimide (PI) film coated with CS solution containing gold ions (Au (III)).					
35220078	1	53	theme	induced	228:234	arg1	electrode					288:296	a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	211:296	a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	211:296	We developed a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode.					
35220078	5	54	theme	blood	680:684	arg1	serum					686:690	blood serum	680:690	blood serum	680:690	To enable on-site monitoring of uric acid (UA) in blood serum by differential pulse adsorptive stripping voltammetry (DPAdSV), the electrode was coupled to a portable potentiostat connected to a cellphone to control and monitor analysis.					
35220078	10	55	theme	hospital	1383:1390	arg1	method					1411:1416	the hospital standard enzymatic method	1379:1416	the hospital standard enzymatic method	1379:1416	The analysis results were in agreement with those of the hospital standard enzymatic method.					
35220078	3	56	theme	3D	459:460	arg1	composite					471:479	the induced 3D graphene composite	447:479	the induced 3D graphene composite	447:479	The AuNPs decorating the induced 3D graphene composite were uniformly distributed.					
35220078	1	57	theme	graphene-chitosan-gold	236:257	arg1	electrode					288:296	a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	211:296	a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	211:296	We developed a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode.					
35220078	0	58	theme	porous	75:80	arg1	electrode					91:99	gold nanoparticle/chitosan/ porous graphene electrode	47:99	gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection	47:123	Facile fabrication of a flexible laser induced gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection.					
35220078	1	59	theme	simple	141:146	arg1	fabrication					196:206	a simple, inexpensive, environmentally friendly one-step fabrication	139:206	a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	139:296	We developed a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode.					
35220078	1	60	theme	nanoparticles	259:271	arg1	electrode					288:296	a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	211:296	a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode	211:296	We developed a simple, inexpensive, environmentally friendly one-step fabrication of a flexible laser induced graphene-chitosan-gold nanoparticles (LIG-CS-AuNPs) electrode.					
35220078	0	61	theme	nanoparticle/chitosan/	52:73	arg1	electrode					91:99	gold nanoparticle/chitosan/ porous graphene electrode	47:99	gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection	47:123	Facile fabrication of a flexible laser induced gold nanoparticle/chitosan/ porous graphene electrode for uric acid detection.					
35220078	4	62	theme	UA	626:627	arg1	oxidation					613:621	the oxidation	609:621	the oxidation of UA	609:627	The electrode exhibited good electrical conductivity and excellent electrocatalytic activity toward the oxidation of UA.					
37059523	2	0	theme	PVA	443:445	arg1	chains					447:452	linear PVA chains	436:452	linear PVA chains	436:452	A strong intermolecular network architecture was formed thanks to the introduction of linear PVA chains, which can efficiently interpenetrate the glutaraldehyde(GA)-crosslinked HTCC chains.					
37059523	1	1	theme	chitosan	285:292	arg1	chloride					294:301	N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride	245:301	N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride	245:301	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	1	1	theme	chitosan	285:292	arg1	HTCC					304:307	HTCC	304:307	HTCC	304:307	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	9	2	theme	aerogel	1464:1470	arg1	filters					1472:1478	aerogel filters	1464:1478	aerogel filters	1464:1478	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol has a high application potential.					
37059523	2	3	theme	linear	436:441	arg1	chains					447:452	linear PVA chains	436:452	linear PVA chains	436:452	A strong intermolecular network architecture was formed thanks to the introduction of linear PVA chains, which can efficiently interpenetrate the glutaraldehyde(GA)-crosslinked HTCC chains.					
37059523	9	4	theme	filters	1472:1478	arg1	concept					1453:1459	The presented concept	1439:1459	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol	1439:1545	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol has a high application potential.					
37059523	3	5	theme	scanning	601:608	arg1	SEM					631:633	SEM	631:633	SEM	631:633	The morphology of the obtained structures was examined using scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
37059523	3	5	theme	scanning	601:608	arg1	microscopy					619:628	scanning electron microscopy	601:628	scanning electron microscopy (SEM)	601:634	The morphology of the obtained structures was examined using scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
37059523	0	6	theme	efficient	77:85	arg1	capturing					87:95	efficient capturing	77:95	efficient capturing of viruses	77:106	Aerogels based on cationically modified chitosan and poly(vinyl alcohol) for efficient capturing of viruses.					
37059523	3	7	theme	atomic	640:645	arg1	AFM					665:667	AFM	665:667	AFM	665:667	The morphology of the obtained structures was examined using scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
37059523	3	7	theme	atomic	640:645	arg1	microscopy					653:662	atomic force microscopy	640:662	atomic force microscopy (AFM)	640:668	The morphology of the obtained structures was examined using scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
37059523	6	8	theme	3-trimethylammonium	1113:1131	arg1	groups					1133:1138	cationic 3-trimethylammonium groups	1104:1138	cationic 3-trimethylammonium groups	1104:1138	The results obtained from the XPS analysis showed the presence of cationic 3-trimethylammonium groups on the surface of the aerogel, which can interact with viral capsid proteins.					
37059523	1	9	theme	cationic	212:219	arg1	derivative					221:230	the cationic derivative	208:230	the cationic derivative of chitosan	208:242	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	6	10	theme	cationic	1104:1111	arg1	groups					1133:1138	cationic 3-trimethylammonium groups	1104:1138	cationic 3-trimethylammonium groups	1104:1138	The results obtained from the XPS analysis showed the presence of cationic 3-trimethylammonium groups on the surface of the aerogel, which can interact with viral capsid proteins.					
37059523	5	11	with	aerogels	831:838	arg1	more					845:848	more	845:848	more	845:848	New aerogels with more than twice as much developed micro- and mesopore space and BET-specific surface area were obtained concerning the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA).					
37059523	9	12	theme	virus	1484:1488	arg1	capture					1490:1496	virus capture	1484:1496	virus capture based on modified chitosan and polyvinyl alcohol	1484:1545	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol has a high application potential.					
37059523	4	13	theme	X-ray	787:791	arg1	XPS					821:823	XPS	821:823	XPS	821:823	The aerogels and modified polymers' elemental composition (including the chemical environment) was determined using X-ray photoelectron spectroscopy (XPS).					
37059523	4	13	theme	X-ray	787:791	arg1	spectroscopy					807:818	X-ray photoelectron spectroscopy	787:818	X-ray photoelectron spectroscopy (XPS)	787:824	The aerogels and modified polymers' elemental composition (including the chemical environment) was determined using X-ray photoelectron spectroscopy (XPS).					
37059523	5	14	theme	BET-specific	909:920	arg1	area					930:933	BET-specific surface area	909:933	BET-specific surface area	909:933	New aerogels with more than twice as much developed micro- and mesopore space and BET-specific surface area were obtained concerning the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA).					
37059523	2	15	theme	strong	352:357	arg1	architecture					382:393	A strong intermolecular network architecture	350:393	A strong intermolecular network architecture	350:393	A strong intermolecular network architecture was formed thanks to the introduction of linear PVA chains, which can efficiently interpenetrate the glutaraldehyde(GA)-crosslinked HTCC chains.					
37059523	6	16	theme	capsid	1201:1206	arg1	proteins					1208:1215	viral capsid proteins	1195:1215	viral capsid proteins	1195:1215	The results obtained from the XPS analysis showed the presence of cationic 3-trimethylammonium groups on the surface of the aerogel, which can interact with viral capsid proteins.					
37059523	9	17	theme	high	1553:1556	arg1	potential					1570:1578	a high application potential	1551:1578	a high application potential	1551:1578	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol has a high application potential.					
37059523	4	18	theme	elemental	707:715	arg1	composition					717:727	The aerogels and modified polymers' elemental composition	671:727	The aerogels and modified polymers' elemental composition (including the chemical environment)	671:764	The aerogels and modified polymers' elemental composition (including the chemical environment) was determined using X-ray photoelectron spectroscopy (XPS).					
37059523	1	19	theme	new	139:141	arg1	bioaerogel					153:162	a new filtering bioaerogel	137:162	a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC)	137:308	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	6	20	theme	viral	1195:1199	arg1	proteins					1208:1215	viral capsid proteins	1195:1215	viral capsid proteins	1195:1215	The results obtained from the XPS analysis showed the presence of cationic 3-trimethylammonium groups on the surface of the aerogel, which can interact with viral capsid proteins.					
37059523	5	21	theme	developed	869:877	arg1	micro-					879:884	much developed micro-	864:884	much developed micro-	864:884	New aerogels with more than twice as much developed micro- and mesopore space and BET-specific surface area were obtained concerning the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA).					
37059523	1	22	theme	filtering	143:151	arg1	bioaerogel					153:162	a new filtering bioaerogel	137:162	a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC)	137:308	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	6	23	from	presence	1092:1099	arg1	surface					1147:1153	the surface	1143:1153	the surface of the aerogel, which can interact with viral capsid proteins	1143:1215	The results obtained from the XPS analysis showed the presence of cationic 3-trimethylammonium groups on the surface of the aerogel, which can interact with viral capsid proteins.					
37059523	4	24	theme	photoelectron	793:805	arg1	XPS					821:823	XPS	821:823	XPS	821:823	The aerogels and modified polymers' elemental composition (including the chemical environment) was determined using X-ray photoelectron spectroscopy (XPS).					
37059523	4	24	theme	photoelectron	793:805	arg1	spectroscopy					807:818	X-ray photoelectron spectroscopy	787:818	X-ray photoelectron spectroscopy (XPS)	787:824	The aerogels and modified polymers' elemental composition (including the chemical environment) was determined using X-ray photoelectron spectroscopy (XPS).					
37059523	9	25	theme	modified	1507:1514	arg1	chitosan					1516:1523	modified chitosan	1507:1523	modified chitosan	1507:1523	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol has a high application potential.					
37059523	2	26	link	-crosslinked	514:525	arg1	chains					532:537	the glutaraldehyde(GA)-crosslinked HTCC chains	492:537	the glutaraldehyde(GA)-crosslinked HTCC chains	492:537	A strong intermolecular network architecture was formed thanks to the introduction of linear PVA chains, which can efficiently interpenetrate the glutaraldehyde(GA)-crosslinked HTCC chains.					
37059523	3	27	theme	electron	610:617	arg1	SEM					631:633	SEM	631:633	SEM	631:633	The morphology of the obtained structures was examined using scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
37059523	3	27	theme	electron	610:617	arg1	microscopy					619:628	scanning electron microscopy	601:628	scanning electron microscopy (SEM)	601:634	The morphology of the obtained structures was examined using scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
37059523	7	28	theme	line	1313:1316	arg1	cells					1293:1297	fibroblast cells	1282:1297	fibroblast cells of the NIH3T3 line	1282:1316	No cytotoxic effect of HTCC/GA/PVA aerogel was also observed on fibroblast cells of the NIH3T3 line.					
37059523	1	29	theme	chitosan	235:242	arg1	PVA					199:201	PVA	199:201	PVA	199:201	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	1	29	theme	chitosan	235:242	arg1	alcohol					190:196	linear polyvinyl alcohol	173:196	linear polyvinyl alcohol (PVA)	173:202	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	1	29	theme	chitosan	235:242	arg1	derivative					221:230	the cationic derivative	208:230	the cationic derivative of chitosan	208:242	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	4	30	theme	modified	688:695	arg1	polymers					697:704	modified polymers	688:704	modified polymers	688:704	The aerogels and modified polymers' elemental composition (including the chemical environment) was determined using X-ray photoelectron spectroscopy (XPS).					
37059523	9	31	theme	polyvinyl	1529:1537	arg1	alcohol					1539:1545	polyvinyl alcohol	1529:1545	polyvinyl alcohol	1529:1545	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol has a high application potential.					
37059523	2	32	theme	network	374:380	arg1	architecture					382:393	A strong intermolecular network architecture	350:393	A strong intermolecular network architecture	350:393	A strong intermolecular network architecture was formed thanks to the introduction of linear PVA chains, which can efficiently interpenetrate the glutaraldehyde(GA)-crosslinked HTCC chains.					
37059523	8	33	from	suspension	1427:1436	arg1	MHV					1417:1419	MHV	1417:1419	MHV	1417:1419	Furthermore, the HTCC/GA/PVA aerogel has been shown that efficiently traps mouse hepatitis virus (MHV) from suspension.					
37059523	8	33	from	suspension	1427:1436	arg1	virus					1410:1414	mouse hepatitis virus	1394:1414	mouse hepatitis virus (MHV) from suspension	1394:1436	Furthermore, the HTCC/GA/PVA aerogel has been shown that efficiently traps mouse hepatitis virus (MHV) from suspension.					
37059523	0	34	theme	viruses	100:106	arg1	capturing					87:95	efficient capturing	77:95	efficient capturing of viruses	77:106	Aerogels based on cationically modified chitosan and poly(vinyl alcohol) for efficient capturing of viruses.					
37059523	1	35	theme	potential	317:325	arg1	application					337:347	a potential antiviral application	315:347	a potential antiviral application	315:347	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	5	36	theme	mesopore	890:897	arg1	space					899:903	mesopore space	890:903	mesopore space	890:903	New aerogels with more than twice as much developed micro- and mesopore space and BET-specific surface area were obtained concerning the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA).					
37059523	5	37	theme	New	827:829	arg1	aerogels					831:838	New aerogels	827:838	New aerogels with more than twice as much developed micro- and mesopore space	827:903	New aerogels with more than twice as much developed micro- and mesopore space and BET-specific surface area were obtained concerning the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA).					
37059523	2	38	theme	intermolecular	359:372	arg1	architecture					382:393	A strong intermolecular network architecture	350:393	A strong intermolecular network architecture	350:393	A strong intermolecular network architecture was formed thanks to the introduction of linear PVA chains, which can efficiently interpenetrate the glutaraldehyde(GA)-crosslinked HTCC chains.					
37059523	1	39	theme	N-[	245:247	arg1	chloride					294:301	N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride	245:301	N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride	245:301	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	1	39	theme	N-[	245:247	arg1	HTCC					304:307	HTCC	304:307	HTCC	304:307	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	1	40	theme	antiviral	327:335	arg1	application					337:347	a potential antiviral application	315:347	a potential antiviral application	315:347	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	8	41	theme	HTCC/GA/PVA	1336:1346	arg1	aerogel					1348:1354	the HTCC/GA/PVA aerogel	1332:1354	the HTCC/GA/PVA aerogel	1332:1354	Furthermore, the HTCC/GA/PVA aerogel has been shown that efficiently traps mouse hepatitis virus (MHV) from suspension.					
37059523	1	42	theme	linear	173:178	arg1	PVA					199:201	PVA	199:201	PVA	199:201	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	1	42	theme	linear	173:178	arg1	alcohol					190:196	linear polyvinyl alcohol	173:196	linear polyvinyl alcohol (PVA)	173:202	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	0	43	theme	modified	31:38	arg1	chitosan					40:47	cationically modified chitosan	18:47	cationically modified chitosan	18:47	Aerogels based on cationically modified chitosan and poly(vinyl alcohol) for efficient capturing of viruses.					
37059523	9	44	theme	application	1558:1568	arg1	potential					1570:1578	a high application potential	1551:1578	a high application potential	1551:1578	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol has a high application potential.					
37059523	1	45	dep	alcohol	190:196	arg1	chloride					294:301	N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride	245:301	N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride	245:301	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	1	45	dep	alcohol	190:196	arg1	HTCC					304:307	HTCC	304:307	HTCC	304:307	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	5	46	theme	starting	964:971	arg1	aerogel					989:995	the starting sample chitosan aerogel	960:995	the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA)	960:1035	New aerogels with more than twice as much developed micro- and mesopore space and BET-specific surface area were obtained concerning the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA).					
37059523	8	47	theme	mouse	1394:1398	arg1	MHV					1417:1419	MHV	1417:1419	MHV	1417:1419	Furthermore, the HTCC/GA/PVA aerogel has been shown that efficiently traps mouse hepatitis virus (MHV) from suspension.					
37059523	8	47	theme	mouse	1394:1398	arg1	virus					1410:1414	mouse hepatitis virus	1394:1414	mouse hepatitis virus (MHV) from suspension	1394:1436	Furthermore, the HTCC/GA/PVA aerogel has been shown that efficiently traps mouse hepatitis virus (MHV) from suspension.					
37059523	1	48	theme	polyvinyl	180:188	arg1	PVA					199:201	PVA	199:201	PVA	199:201	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	1	48	theme	polyvinyl	180:188	arg1	alcohol					190:196	linear polyvinyl alcohol	173:196	linear polyvinyl alcohol (PVA)	173:202	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	1	49	theme	2-hydroxy-3-trimethylamine	249:274	arg1	chloride					294:301	N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride	245:301	N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride	245:301	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	1	49	theme	2-hydroxy-3-trimethylamine	249:274	arg1	HTCC					304:307	HTCC	304:307	HTCC	304:307	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	8	50	theme	hepatitis	1400:1408	arg1	MHV					1417:1419	MHV	1417:1419	MHV	1417:1419	Furthermore, the HTCC/GA/PVA aerogel has been shown that efficiently traps mouse hepatitis virus (MHV) from suspension.					
37059523	8	50	theme	hepatitis	1400:1408	arg1	virus					1410:1414	mouse hepatitis virus	1394:1414	mouse hepatitis virus (MHV) from suspension	1394:1436	Furthermore, the HTCC/GA/PVA aerogel has been shown that efficiently traps mouse hepatitis virus (MHV) from suspension.					
37059523	5	51	theme	sample	973:978	arg1	aerogel					989:995	the starting sample chitosan aerogel	960:995	the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA)	960:1035	New aerogels with more than twice as much developed micro- and mesopore space and BET-specific surface area were obtained concerning the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA).					
37059523	7	52	theme	cytotoxic	1221:1229	arg1	effect					1231:1236	No cytotoxic effect	1218:1236	No cytotoxic effect of HTCC/GA/PVA aerogel	1218:1259	No cytotoxic effect of HTCC/GA/PVA aerogel was also observed on fibroblast cells of the NIH3T3 line.					
37059523	9	53	contain	has	1547:1549	arg2	potential					1570:1578	a high application potential	1551:1578	a high application potential	1551:1578	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol has a high application potential.					
37059523	9	53	contain	has	1547:1549	arg1	concept					1453:1459	The presented concept	1439:1459	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol	1439:1545	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol has a high application potential.					
37059523	2	54	theme	HTCC	527:530	arg1	chains					532:537	the glutaraldehyde(GA)-crosslinked HTCC chains	492:537	the glutaraldehyde(GA)-crosslinked HTCC chains	492:537	A strong intermolecular network architecture was formed thanks to the introduction of linear PVA chains, which can efficiently interpenetrate the glutaraldehyde(GA)-crosslinked HTCC chains.					
37059523	5	55	theme	chitosan	980:987	arg1	aerogel					989:995	the starting sample chitosan aerogel	960:995	the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA)	960:1035	New aerogels with more than twice as much developed micro- and mesopore space and BET-specific surface area were obtained concerning the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA).					
37059523	7	56	theme	fibroblast	1282:1291	arg1	cells					1293:1297	fibroblast cells	1282:1297	fibroblast cells of the NIH3T3 line	1282:1316	No cytotoxic effect of HTCC/GA/PVA aerogel was also observed on fibroblast cells of the NIH3T3 line.					
37059523	1	57	theme	propyl	277:282	arg1	chloride					294:301	N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride	245:301	N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride	245:301	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	1	57	theme	propyl	277:282	arg1	HTCC					304:307	HTCC	304:307	HTCC	304:307	In this study, we developed a new filtering bioaerogel based on linear polyvinyl alcohol (PVA) and the cationic derivative of chitosan (N-[(2-hydroxy-3-trimethylamine) propyl] chitosan chloride, HTCC) with a potential antiviral application.					
37059523	2	58	theme	-crosslinked	514:525	arg1	chains					532:537	the glutaraldehyde(GA)-crosslinked HTCC chains	492:537	the glutaraldehyde(GA)-crosslinked HTCC chains	492:537	A strong intermolecular network architecture was formed thanks to the introduction of linear PVA chains, which can efficiently interpenetrate the glutaraldehyde(GA)-crosslinked HTCC chains.					
37059523	6	59	theme	aerogel	1162:1168	arg1	surface					1147:1153	the surface	1143:1153	the surface of the aerogel, which can interact with viral capsid proteins	1143:1215	The results obtained from the XPS analysis showed the presence of cationic 3-trimethylammonium groups on the surface of the aerogel, which can interact with viral capsid proteins.					
37059523	3	60	theme	structures	571:580	arg1	morphology					544:553	The morphology	540:553	The morphology of the obtained structures	540:580	The morphology of the obtained structures was examined using scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
37059523	6	61	theme	XPS	1068:1070	arg1	analysis					1072:1079	the XPS analysis	1064:1079	the XPS analysis	1064:1079	The results obtained from the XPS analysis showed the presence of cationic 3-trimethylammonium groups on the surface of the aerogel, which can interact with viral capsid proteins.					
37059523	5	62	theme	surface	922:928	arg1	area					930:933	BET-specific surface area	909:933	BET-specific surface area	909:933	New aerogels with more than twice as much developed micro- and mesopore space and BET-specific surface area were obtained concerning the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA).					
37059523	7	63	located	observed	1270:1277	arg2	effect					1231:1236	No cytotoxic effect	1218:1236	No cytotoxic effect of HTCC/GA/PVA aerogel	1218:1259	No cytotoxic effect of HTCC/GA/PVA aerogel was also observed on fibroblast cells of the NIH3T3 line.					
37059523	7	63	located	observed	1270:1277	arg1	cells					1293:1297	fibroblast cells	1282:1297	fibroblast cells of the NIH3T3 line	1282:1316	No cytotoxic effect of HTCC/GA/PVA aerogel was also observed on fibroblast cells of the NIH3T3 line.					
37059523	7	64	theme	NIH3T3	1306:1311	arg1	line					1313:1316	the NIH3T3 line	1302:1316	the NIH3T3 line	1302:1316	No cytotoxic effect of HTCC/GA/PVA aerogel was also observed on fibroblast cells of the NIH3T3 line.					
37059523	9	65	theme	presented	1443:1451	arg1	concept					1453:1459	The presented concept	1439:1459	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol	1439:1545	The presented concept of aerogel filters for virus capture based on modified chitosan and polyvinyl alcohol has a high application potential.					
37059523	0	66	theme	vinyl	58:62	arg1	alcohol					64:70	vinyl alcohol	58:70	vinyl alcohol	58:70	Aerogels based on cationically modified chitosan and poly(vinyl alcohol) for efficient capturing of viruses.					
37059523	0	66	theme	vinyl	58:62	arg1	poly					53:56	poly	53:56	poly(vinyl alcohol)	53:71	Aerogels based on cationically modified chitosan and poly(vinyl alcohol) for efficient capturing of viruses.					
37059523	7	67	theme	HTCC/GA/PVA	1241:1251	arg1	aerogel					1253:1259	HTCC/GA/PVA aerogel	1241:1259	HTCC/GA/PVA aerogel	1241:1259	No cytotoxic effect of HTCC/GA/PVA aerogel was also observed on fibroblast cells of the NIH3T3 line.					
37059523	4	68	theme	chemical	744:751	arg1	environment					753:763	the chemical environment	740:763	the chemical environment	740:763	The aerogels and modified polymers' elemental composition (including the chemical environment) was determined using X-ray photoelectron spectroscopy (XPS).					
37059523	7	69	theme	aerogel	1253:1259	arg1	effect					1231:1236	No cytotoxic effect	1218:1236	No cytotoxic effect of HTCC/GA/PVA aerogel	1218:1259	No cytotoxic effect of HTCC/GA/PVA aerogel was also observed on fibroblast cells of the NIH3T3 line.					
37059523	4	70	dep	composition	717:727	arg1	including					730:738	including	730:738	including the chemical environment	730:763	The aerogels and modified polymers' elemental composition (including the chemical environment) was determined using X-ray photoelectron spectroscopy (XPS).					
37059523	6	71	theme	groups	1133:1138	arg1	presence					1092:1099	the presence	1088:1099	the presence of cationic 3-trimethylammonium groups on the surface of the aerogel, which can interact with viral capsid proteins	1088:1215	The results obtained from the XPS analysis showed the presence of cationic 3-trimethylammonium groups on the surface of the aerogel, which can interact with viral capsid proteins.					
37059523	2	72	theme	chains	447:452	arg1	introduction					420:431	the introduction	416:431	the introduction of linear PVA chains, which can efficiently interpenetrate the glutaraldehyde(GA)-crosslinked HTCC chains	416:537	A strong intermolecular network architecture was formed thanks to the introduction of linear PVA chains, which can efficiently interpenetrate the glutaraldehyde(GA)-crosslinked HTCC chains.					
37059523	3	73	theme	force	647:651	arg1	AFM					665:667	AFM	665:667	AFM	665:667	The morphology of the obtained structures was examined using scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
37059523	3	73	theme	force	647:651	arg1	microscopy					653:662	atomic force microscopy	640:662	atomic force microscopy (AFM)	640:668	The morphology of the obtained structures was examined using scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
37059523	3	74	theme	obtained	562:569	arg1	structures					571:580	the obtained structures	558:580	the obtained structures	558:580	The morphology of the obtained structures was examined using scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
37059523	5	75	with	area	930:933	arg1	more					845:848	more	845:848	more	845:848	New aerogels with more than twice as much developed micro- and mesopore space and BET-specific surface area were obtained concerning the starting sample chitosan aerogel crosslinked by glutaraldehyde (Chit/GA).					
37059523	6	76	attach	presence	1092:1099	arg2	groups					1133:1138	cationic 3-trimethylammonium groups	1104:1138	cationic 3-trimethylammonium groups	1104:1138	The results obtained from the XPS analysis showed the presence of cationic 3-trimethylammonium groups on the surface of the aerogel, which can interact with viral capsid proteins.					
37059523	6	76	attach	presence	1092:1099	arg1	surface					1147:1153	the surface	1143:1153	the surface of the aerogel, which can interact with viral capsid proteins	1143:1215	The results obtained from the XPS analysis showed the presence of cationic 3-trimethylammonium groups on the surface of the aerogel, which can interact with viral capsid proteins.					
36958445	2	0	theme	good	510:513	arg1	biocompatibility					515:530	good biocompatibility	510:530	good biocompatibility	510:530	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	7	1	theme	wound	1530:1534	arg1	dressings					1536:1544	hemostatic wound dressings	1519:1544	hemostatic wound dressings in clinical surgery and emergent treatment	1519:1587	These results demonstrate promising potential of the GelMA-DA/QCS/Gly sponges for applications as hemostatic wound dressings in clinical surgery and emergent treatment.					
36958445	6	2	theme	commercial	1358:1367	arg1	sponge					1388:1393	the commercial Gelatin hemostatic sponge and CS hemostatic sponge	1354:1418	sponge	1388:1393	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	0	3	with	sponges	77:83	arg1	ability					144:150	hemostatic ability	133:150	hemostatic ability	133:150	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.					
36958445	0	3	with	sponges	77:83	arg1	self-adhesion					90:102	self-adhesion	90:102	self-adhesion	90:102	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.					
36958445	0	3	with	sponges	77:83	arg1	activity					119:126	antibacterial activity	105:126	antibacterial activity	105:126	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.					
36958445	4	4	theme	sponge	758:763	arg1	128.36 ± 8.45					818:830	128.36 ± 8.45	818:830	128.36 ± 8.45	818:830	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V% is approximately 128.36 ± 8.45, 125.17 ± 7.18, 138.29 ± 7.94, and 113.83 ± 9.28 kPa for skin, liver, muscle, and fat, respectively.					
36958445	4	4	theme	sponge	758:763	arg1	strength					725:732	The lap shear strength	711:732	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V%	711:799	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V% is approximately 128.36 ± 8.45, 125.17 ± 7.18, 138.29 ± 7.94, and 113.83 ± 9.28 kPa for skin, liver, muscle, and fat, respectively.					
36958445	1	5	theme	bleeding	290:297	arg1	profuse					282:288	profuse	282:288	profuse bleeding in clinical surgery and emergent accident	282:339	It is one of the most emergent challenges to prepare wound dressings for quickly and effectively controlling profuse bleeding in clinical surgery and emergent accident.					
36958445	7	6	theme	hemostatic	1519:1528	arg1	dressings					1536:1544	hemostatic wound dressings	1519:1544	hemostatic wound dressings in clinical surgery and emergent treatment	1519:1587	These results demonstrate promising potential of the GelMA-DA/QCS/Gly sponges for applications as hemostatic wound dressings in clinical surgery and emergent treatment.					
36958445	1	7	theme	most	190:193	arg1	challenges					204:213	the most emergent challenges	186:213	the most emergent challenges	186:213	It is one of the most emergent challenges to prepare wound dressings for quickly and effectively controlling profuse bleeding in clinical surgery and emergent accident.					
36958445	7	8	from	dressings	1536:1544	arg1	surgery					1558:1564	clinical surgery	1549:1564	clinical surgery	1549:1564	These results demonstrate promising potential of the GelMA-DA/QCS/Gly sponges for applications as hemostatic wound dressings in clinical surgery and emergent treatment.					
36958445	7	8	from	dressings	1536:1544	arg1	treatment					1579:1587	emergent treatment	1570:1587	emergent treatment	1570:1587	These results demonstrate promising potential of the GelMA-DA/QCS/Gly sponges for applications as hemostatic wound dressings in clinical surgery and emergent treatment.					
36958445	2	9	theme	antibacterial	555:567	arg1	activity					569:576	antibacterial activity	555:576	antibacterial activity	555:576	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	7	10	theme	promising	1447:1455	arg1	potential					1457:1465	promising potential	1447:1465	promising potential of the GelMA-DA/QCS/Gly sponges for applications as hemostatic wound dressings in clinical surgery and emergent treatment	1447:1587	These results demonstrate promising potential of the GelMA-DA/QCS/Gly sponges for applications as hemostatic wound dressings in clinical surgery and emergent treatment.					
36958445	6	11	theme	GelMA-DA/QCS/Gly	1227:1242	arg1	group					1251:1255	the GelMA-DA/QCS/Gly sponge group	1223:1255	the GelMA-DA/QCS/Gly sponge group	1223:1255	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	5	12	theme	negative	1026:1033	arg1	bacteria					1035:1042	Gram positive and negative bacteria	1008:1042	Gram positive and negative bacteria	1008:1042	The GelMA-DA/QCS/Gly sponge displays better antibacterial activity against Gram positive and negative bacteria than the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	4	13	theme	lap	715:717	arg1	128.36 ± 8.45					818:830	128.36 ± 8.45	818:830	128.36 ± 8.45	818:830	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V% is approximately 128.36 ± 8.45, 125.17 ± 7.18, 138.29 ± 7.94, and 113.83 ± 9.28 kPa for skin, liver, muscle, and fat, respectively.					
36958445	4	13	theme	lap	715:717	arg1	strength					725:732	The lap shear strength	711:732	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V%	711:799	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V% is approximately 128.36 ± 8.45, 125.17 ± 7.18, 138.29 ± 7.94, and 113.83 ± 9.28 kPa for skin, liver, muscle, and fat, respectively.					
36958445	1	14	theme	clinical	302:309	arg1	surgery					311:317	clinical surgery	302:317	clinical surgery	302:317	It is one of the most emergent challenges to prepare wound dressings for quickly and effectively controlling profuse bleeding in clinical surgery and emergent accident.					
36958445	0	15	theme	hemostatic	133:142	arg1	ability					144:150	hemostatic ability	133:150	hemostatic ability	133:150	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.					
36958445	5	16	theme	GelMA-DA/QCS/Gly	937:952	arg1	sponge					954:959	The GelMA-DA/QCS/Gly sponge	933:959	The GelMA-DA/QCS/Gly sponge	933:959	The GelMA-DA/QCS/Gly sponge displays better antibacterial activity against Gram positive and negative bacteria than the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	6	17	theme	hemostatic	1402:1411	arg1	sponge					1413:1418	the commercial Gelatin hemostatic sponge and CS hemostatic sponge	1354:1418	sponge	1413:1418	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	0	18	theme	wound	156:160	arg1	dressings					162:170	wound dressings	156:170	wound dressings	156:170	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.					
36958445	7	19	theme	emergent	1570:1577	arg1	treatment					1579:1587	emergent treatment	1570:1587	emergent treatment	1570:1587	These results demonstrate promising potential of the GelMA-DA/QCS/Gly sponges for applications as hemostatic wound dressings in clinical surgery and emergent treatment.					
36958445	1	20	from	accident	332:339	arg1	bleeding					290:297	bleeding	290:297	bleeding	290:297	It is one of the most emergent challenges to prepare wound dressings for quickly and effectively controlling profuse bleeding in clinical surgery and emergent accident.					
36958445	6	21	theme	liver	1154:1158	arg1	model					1169:1173	rat tail and liver bleeding model	1141:1173	model	1169:1173	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	6	22	theme	sponge	1244:1249	arg1	group					1251:1255	the GelMA-DA/QCS/Gly sponge group	1223:1255	the GelMA-DA/QCS/Gly sponge group	1223:1255	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	1	23	theme	emergent	195:202	arg1	challenges					204:213	the most emergent challenges	186:213	the most emergent challenges	186:213	It is one of the most emergent challenges to prepare wound dressings for quickly and effectively controlling profuse bleeding in clinical surgery and emergent accident.					
36958445	2	24	theme	tissue	533:538	arg1	self-adhesion					540:552	tissue self-adhesion	533:552	tissue self-adhesion	533:552	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	7	25	theme	clinical	1549:1556	arg1	surgery					1558:1564	clinical surgery	1549:1564	clinical surgery	1549:1564	These results demonstrate promising potential of the GelMA-DA/QCS/Gly sponges for applications as hemostatic wound dressings in clinical surgery and emergent treatment.					
36958445	6	26	theme	hemostasis	1189:1198	arg1	time					1200:1203	hemostasis time	1189:1203	hemostasis time	1189:1203	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	0	27	theme	Mussel-inspired	0:14	arg1	sponges					77:83	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges	0:83	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.	0:171	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.					
36958445	6	28	theme	bleeding	1160:1167	arg1	model					1169:1173	rat tail and liver bleeding model	1141:1173	model	1169:1173	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	1	29	theme	challenges	204:213	arg1	challenges					204:213	the most emergent challenges	186:213	the most emergent challenges	186:213	It is one of the most emergent challenges to prepare wound dressings for quickly and effectively controlling profuse bleeding in clinical surgery and emergent accident.					
36958445	1	29	theme	challenges	204:213	arg1	one					179:181	one	179:181	one	179:181	It is one of the most emergent challenges to prepare wound dressings for quickly and effectively controlling profuse bleeding in clinical surgery and emergent accident.					
36958445	0	30	theme	methacrylated	16:28	arg1	sponges					77:83	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges	0:83	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.	0:171	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.					
36958445	6	31	dep	time	1200:1203	arg1	the					1185:1187	the	1185:1187	the	1185:1187	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	2	32	theme	gelatin-dopamine	417:432	arg1	sponges					497:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	2	33	theme	/glycerol	471:479	arg1	sponges					497:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	6	34	from	loss	1215:1218	arg1	group					1251:1255	the GelMA-DA/QCS/Gly sponge group	1223:1255	the GelMA-DA/QCS/Gly sponge group	1223:1255	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	2	35	theme	methacrylated	403:415	arg1	sponges					497:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	0	36	theme	chitosan/glycerin	59:75	arg1	sponges					77:83	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges	0:83	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.	0:171	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.					
36958445	1	37	theme	emergent	323:330	arg1	accident					332:339	emergent accident	323:339	emergent accident	323:339	It is one of the most emergent challenges to prepare wound dressings for quickly and effectively controlling profuse bleeding in clinical surgery and emergent accident.					
36958445	2	38	theme	hemostatic	583:592	arg1	ability					594:600	hemostatic ability	583:600	hemostatic ability	583:600	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	5	39	theme	positive	1013:1020	arg1	bacteria					1035:1042	Gram positive and negative bacteria	1008:1042	Gram positive and negative bacteria	1008:1042	The GelMA-DA/QCS/Gly sponge displays better antibacterial activity against Gram positive and negative bacteria than the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	1	40	theme	wound	226:230	arg1	dressings					232:240	wound dressings	226:240	wound dressings	226:240	It is one of the most emergent challenges to prepare wound dressings for quickly and effectively controlling profuse bleeding in clinical surgery and emergent accident.					
36958445	2	41	theme	QCS	467:469	arg1	sponges					497:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	1	42	from	surgery	311:317	arg1	bleeding					290:297	bleeding	290:297	bleeding	290:297	It is one of the most emergent challenges to prepare wound dressings for quickly and effectively controlling profuse bleeding in clinical surgery and emergent accident.					
36958445	4	43	with	strength	725:732	arg1	content					783:789	the GelMA-DA content	770:789	the GelMA-DA content of 5 W/V%	770:799	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V% is approximately 128.36 ± 8.45, 125.17 ± 7.18, 138.29 ± 7.94, and 113.83 ± 9.28 kPa for skin, liver, muscle, and fat, respectively.					
36958445	4	44	theme	%	799:799	arg1	content					783:789	the GelMA-DA content	770:789	the GelMA-DA content of 5 W/V%	770:799	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V% is approximately 128.36 ± 8.45, 125.17 ± 7.18, 138.29 ± 7.94, and 113.83 ± 9.28 kPa for skin, liver, muscle, and fat, respectively.					
36958445	2	45	theme	chitosan	457:464	arg1	sponges					497:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	1	46	from	bleeding	290:297	arg1	accident					332:339	emergent accident	323:339	emergent accident	323:339	It is one of the most emergent challenges to prepare wound dressings for quickly and effectively controlling profuse bleeding in clinical surgery and emergent accident.					
36958445	1	46	from	bleeding	290:297	arg1	surgery					311:317	clinical surgery	302:317	clinical surgery	302:317	It is one of the most emergent challenges to prepare wound dressings for quickly and effectively controlling profuse bleeding in clinical surgery and emergent accident.					
36958445	6	47	theme	Animal	1116:1121	arg1	experiments					1123:1133	Animal experiments	1116:1133	Animal experiments using rat tail and liver bleeding model	1116:1173	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	3	48	theme	GelMA-DA/QCS/Gly	625:640	arg1	sponges					642:648	the GelMA-DA/QCS/Gly sponges	621:648	the GelMA-DA/QCS/Gly sponges	621:648	Results show that the GelMA-DA/QCS/Gly sponges display good biocompatibility and water absorption capacity.					
36958445	4	49	theme	shear	719:723	arg1	128.36 ± 8.45					818:830	128.36 ± 8.45	818:830	128.36 ± 8.45	818:830	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V% is approximately 128.36 ± 8.45, 125.17 ± 7.18, 138.29 ± 7.94, and 113.83 ± 9.28 kPa for skin, liver, muscle, and fat, respectively.					
36958445	4	49	theme	shear	719:723	arg1	strength					725:732	The lap shear strength	711:732	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V%	711:799	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V% is approximately 128.36 ± 8.45, 125.17 ± 7.18, 138.29 ± 7.94, and 113.83 ± 9.28 kPa for skin, liver, muscle, and fat, respectively.					
36958445	2	50	theme	/quaternized	444:455	arg1	sponges					497:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	2	51	theme	GelMA-DA	435:442	arg1	sponges					497:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	2	52	theme	composite	487:495	arg1	sponges					497:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	3	53	theme	water	684:688	arg1	capacity					701:708	water absorption capacity	684:708	water absorption capacity	684:708	Results show that the GelMA-DA/QCS/Gly sponges display good biocompatibility and water absorption capacity.					
36958445	5	54	theme	CS	1094:1095	arg1	sponge					1108:1113	CS hemostatic sponge	1094:1113	CS hemostatic sponge	1094:1113	The GelMA-DA/QCS/Gly sponge displays better antibacterial activity against Gram positive and negative bacteria than the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	3	55	theme	absorption	690:699	arg1	capacity					701:708	water absorption capacity	684:708	water absorption capacity	684:708	Results show that the GelMA-DA/QCS/Gly sponges display good biocompatibility and water absorption capacity.					
36958445	5	56	theme	hemostatic	1097:1106	arg1	sponge					1108:1113	CS hemostatic sponge	1094:1113	CS hemostatic sponge	1094:1113	The GelMA-DA/QCS/Gly sponge displays better antibacterial activity against Gram positive and negative bacteria than the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	2	57	theme	Gly	482:484	arg1	sponges					497:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges	403:503	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	6	58	theme	rat	1141:1143	arg1	tail					1145:1148	rat tail and liver bleeding model	1141:1173	tail	1145:1148	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	5	59	theme	better	970:975	arg1	activity					991:998	better antibacterial activity	970:998	better antibacterial activity against Gram positive and negative bacteria than the commercial Gelatin hemostatic sponge and CS hemostatic sponge	970:1113	The GelMA-DA/QCS/Gly sponge displays better antibacterial activity against Gram positive and negative bacteria than the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	3	60	theme	good	658:661	arg1	biocompatibility					663:678	good biocompatibility	658:678	good biocompatibility	658:678	Results show that the GelMA-DA/QCS/Gly sponges display good biocompatibility and water absorption capacity.					
36958445	6	61	theme	hemostatic	1377:1386	arg1	sponge					1388:1393	the commercial Gelatin hemostatic sponge and CS hemostatic sponge	1354:1418	sponge	1388:1393	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	5	62	theme	commercial	1053:1062	arg1	sponge					1083:1088	the commercial Gelatin hemostatic sponge	1049:1088	the commercial Gelatin hemostatic sponge	1049:1088	The GelMA-DA/QCS/Gly sponge displays better antibacterial activity against Gram positive and negative bacteria than the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	6	63	from	time	1200:1203	arg1	group					1251:1255	the GelMA-DA/QCS/Gly sponge group	1223:1255	the GelMA-DA/QCS/Gly sponge group	1223:1255	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	2	64	theme	novel	358:362	arg1	strategy					364:371	a novel strategy	356:371	a novel strategy	356:371	In this work, a novel strategy has been developed to prepare methacrylated gelatin-dopamine (GelMA-DA)/quaternized chitosan (QCS)/glycerol (Gly) composite sponges with good biocompatibility, tissue self-adhesion, antibacterial activity, and hemostatic ability.					
36958445	4	65	theme	GelMA-DA/QCS/Gly	741:756	arg1	sponge					758:763	the GelMA-DA/QCS/Gly sponge	737:763	the GelMA-DA/QCS/Gly sponge	737:763	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V% is approximately 128.36 ± 8.45, 125.17 ± 7.18, 138.29 ± 7.94, and 113.83 ± 9.28 kPa for skin, liver, muscle, and fat, respectively.					
36958445	6	66	theme	CS	1399:1400	arg1	sponge					1413:1418	the commercial Gelatin hemostatic sponge and CS hemostatic sponge	1354:1418	sponge	1413:1418	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	6	67	theme	Gelatin	1369:1375	arg1	sponge					1388:1393	the commercial Gelatin hemostatic sponge and CS hemostatic sponge	1354:1418	sponge	1388:1393	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	5	68	theme	Gelatin	1064:1070	arg1	sponge					1083:1088	the commercial Gelatin hemostatic sponge	1049:1088	the commercial Gelatin hemostatic sponge	1049:1088	The GelMA-DA/QCS/Gly sponge displays better antibacterial activity against Gram positive and negative bacteria than the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	7	69	theme	sponges	1491:1497	arg1	potential					1457:1465	promising potential	1447:1465	promising potential of the GelMA-DA/QCS/Gly sponges for applications as hemostatic wound dressings in clinical surgery and emergent treatment	1447:1587	These results demonstrate promising potential of the GelMA-DA/QCS/Gly sponges for applications as hemostatic wound dressings in clinical surgery and emergent treatment.					
36958445	4	70	theme	GelMA-DA	774:781	arg1	content					783:789	the GelMA-DA content	770:789	the GelMA-DA content of 5 W/V%	770:799	The lap shear strength of the GelMA-DA/QCS/Gly sponge with the GelMA-DA content of 5 W/V% is approximately 128.36 ± 8.45, 125.17 ± 7.18, 138.29 ± 7.94, and 113.83 ± 9.28 kPa for skin, liver, muscle, and fat, respectively.					
36958445	5	71	theme	antibacterial	977:989	arg1	activity					991:998	better antibacterial activity	970:998	better antibacterial activity against Gram positive and negative bacteria than the commercial Gelatin hemostatic sponge and CS hemostatic sponge	970:1113	The GelMA-DA/QCS/Gly sponge displays better antibacterial activity against Gram positive and negative bacteria than the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	5	72	theme	hemostatic	1072:1081	arg1	sponge					1083:1088	the commercial Gelatin hemostatic sponge	1049:1088	the commercial Gelatin hemostatic sponge	1049:1088	The GelMA-DA/QCS/Gly sponge displays better antibacterial activity against Gram positive and negative bacteria than the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	0	73	theme	antibacterial	105:117	arg1	activity					119:126	antibacterial activity	105:126	antibacterial activity	105:126	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.					
36958445	6	74	theme	blood	1209:1213	arg1	loss					1215:1218	blood loss	1209:1218	blood loss	1209:1218	Animal experiments using rat tail and liver bleeding model show that the hemostasis time and blood loss in the GelMA-DA/QCS/Gly sponge group is approximately 33.3 ± 6.7 s and 0.19 ± 0.05 g, respectively, which is also better than that of the commercial Gelatin hemostatic sponge and CS hemostatic sponge.					
36958445	0	75	theme	gelatin-dopamine/quaternized	30:57	arg1	sponges					77:83	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges	0:83	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.	0:171	Mussel-inspired methacrylated gelatin-dopamine/quaternized chitosan/glycerin sponges with self-adhesion, antibacterial activity, and hemostatic ability for wound dressings.					
36958445	7	76	theme	GelMA-DA/QCS/Gly	1474:1489	arg1	sponges					1491:1497	the GelMA-DA/QCS/Gly sponges	1470:1497	the GelMA-DA/QCS/Gly sponges	1470:1497	These results demonstrate promising potential of the GelMA-DA/QCS/Gly sponges for applications as hemostatic wound dressings in clinical surgery and emergent treatment.					
36475916	9	0	theme	pectin	1666:1671	arg1	ingestions					1673:1682	pectin ingestions	1666:1682	pectin ingestions	1666:1682	IMPORTANCE Collective studies indicate that pectin degradation promotes extensive microorganisms that can be involved in pectin degradation directly or indirectly, or benefit from the altered physiological conditions caused by pectin ingestions.					
36475916	10	1	theme	microbial	1731:1739	arg1	taxa					1741:1744	gut microbial taxa	1727:1744	gut microbial taxa	1727:1744	Our study focuses on effects of pectin on gut microbial taxa and functions, as well as its interactions with altered environmental features.					
36475916	11	2	theme	microbial	2050:2058	arg1	communities					2060:2070	microbial communities	2050:2070	microbial communities	2050:2070	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	6	3	theme	pig	864:866	arg1	model					868:872	a fistula pig model	854:872	a fistula pig model	854:872	Furthermore, we first confirmed that PEC decreased fecal redox potential in a fistula pig model, which could be supported by the enrichment of antioxidants (e.g., inosine) in feces.					
36475916	3	4	theme	taxa	530:533	arg1	abundances					500:509	the abundances	496:509	the abundances of fungal keystone taxa	496:533	Here, metatranscriptome sequencing revealed that a pectin-enriched diet (PEC) decreased the abundances of fungal keystone taxa (e.g., amino acid-producing Kazachstania spp.)					
36475916	9	5	theme	Collective	1450:1459	arg1	studies					1461:1467	IMPORTANCE Collective studies	1439:1467	IMPORTANCE Collective studies	1439:1467	IMPORTANCE Collective studies indicate that pectin degradation promotes extensive microorganisms that can be involved in pectin degradation directly or indirectly, or benefit from the altered physiological conditions caused by pectin ingestions.					
36475916	8	6	theme	pig	1237:1239	arg1	hindgut					1241:1247	pig hindgut	1237:1247	pig hindgut	1237:1247	Overall, these results demonstrate that pectin decreases the redox potential in pig hindgut to modulate microbial composition and functions, and specific microorganisms generate reducing agents in the course of pectin degradation to decrease redox potential of microbial ecosystem.					
36475916	7	7	theme	inosine	1033:1039	arg1	biosynthesis					1041:1052	inosine biosynthesis	1033:1052	inosine biosynthesis	1033:1052	Fecal metagenome analysis disclosed that certain microbial taxa promoted inosine biosynthesis from pectin degradation, including Prevotella, which plays an essential role in pectin biodegradation.					
36475916	3	8	theme	fungal	514:519	arg1	taxa					530:533	fungal keystone taxa	514:533	fungal keystone taxa	514:533	Here, metatranscriptome sequencing revealed that a pectin-enriched diet (PEC) decreased the abundances of fungal keystone taxa (e.g., amino acid-producing Kazachstania spp.)					
36475916	1	9	theme	vast	165:168	arg1	range					170:174	a vast range	163:174	a vast range of plants	163:184	Pectin exists in a vast range of plants and has a long history of acting as a functional food additive with potential prebiotic effects on intestinal health.					
36475916	8	10	theme	ecosystem	1428:1436	arg1	potential					1405:1413	redox potential	1399:1413	redox potential of microbial ecosystem	1399:1436	Overall, these results demonstrate that pectin decreases the redox potential in pig hindgut to modulate microbial composition and functions, and specific microorganisms generate reducing agents in the course of pectin degradation to decrease redox potential of microbial ecosystem.					
36475916	11	11	theme	pectin-induced	1850:1863	arg1	shifts					1877:1882	pectin-induced proreducing shifts	1850:1882	pectin-induced proreducing shifts on colon microbial taxa and functions	1850:1920	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	1	12	contain	has	190:192	arg2	history					201:207	a long history	194:207	a long history of acting as a functional food additive with potential prebiotic effects on intestinal health	194:301	Pectin exists in a vast range of plants and has a long history of acting as a functional food additive with potential prebiotic effects on intestinal health.					
36475916	1	12	contain	has	190:192	arg1	Pectin					146:151	Pectin	146:151	Pectin	146:151	Pectin exists in a vast range of plants and has a long history of acting as a functional food additive with potential prebiotic effects on intestinal health.					
36475916	8	13	from	potential	1224:1232	arg1	hindgut					1241:1247	pig hindgut	1237:1247	pig hindgut	1237:1247	Overall, these results demonstrate that pectin decreases the redox potential in pig hindgut to modulate microbial composition and functions, and specific microorganisms generate reducing agents in the course of pectin degradation to decrease redox potential of microbial ecosystem.					
36475916	9	14	theme	pectin	1483:1488	arg1	degradation					1490:1500	pectin degradation	1483:1500	pectin degradation	1483:1500	IMPORTANCE Collective studies indicate that pectin degradation promotes extensive microorganisms that can be involved in pectin degradation directly or indirectly, or benefit from the altered physiological conditions caused by pectin ingestions.					
36475916	8	15	theme	microbial	1261:1269	arg1	composition					1271:1281	microbial composition	1261:1281	microbial composition	1261:1281	Overall, these results demonstrate that pectin decreases the redox potential in pig hindgut to modulate microbial composition and functions, and specific microorganisms generate reducing agents in the course of pectin degradation to decrease redox potential of microbial ecosystem.					
36475916	7	16	theme	certain	1001:1007	arg1	taxa					1019:1022	certain microbial taxa	1001:1022	certain microbial taxa	1001:1022	Fecal metagenome analysis disclosed that certain microbial taxa promoted inosine biosynthesis from pectin degradation, including Prevotella, which plays an essential role in pectin biodegradation.					
36475916	10	17	theme	environmental	1802:1814	arg1	features					1816:1823	altered environmental features	1794:1823	altered environmental features	1794:1823	Our study focuses on effects of pectin on gut microbial taxa and functions, as well as its interactions with altered environmental features.					
36475916	4	18	theme	sulfate-reducing	673:688	arg1	spp.					704:707	sulfate-reducing Desulfovibrio spp.	673:707	sulfate-reducing Desulfovibrio spp.	673:707	and their genes involved in oxidative phosphorylation, while it increased the abundance of sulfate-reducing Desulfovibrio spp., and methane-producing Methanobrevibacter spp.					
36475916	0	19	theme	Pig	135:137	arg1	Model					139:143	a Pig Model	133:143	a Pig Model	133:143	Reduction of Redox Potential Exerts a Key Role in Modulating Gut Microbial Taxa and Function by Dietary Supplementation of Pectin in a Pig Model.					
36475916	13	20	theme	microbiota	2369:2378	arg1	modulation					2351:2360	modulation	2351:2360	modulation of gut microbiota	2351:2378	In general, this investigation proposes new insights into the pectin-modulating gut microbial ecosystem and also provides new perspectives for targeting modulation of gut microbiota.					
36475916	13	21	theme	gut	2365:2367	arg1	microbiota					2369:2378	gut microbiota	2365:2378	gut microbiota	2365:2378	In general, this investigation proposes new insights into the pectin-modulating gut microbial ecosystem and also provides new perspectives for targeting modulation of gut microbiota.					
36475916	3	22	theme	Kazachstania	563:574	arg1	spp					576:578	amino acid-producing Kazachstania spp	542:578	amino acid-producing Kazachstania spp.	542:579	Here, metatranscriptome sequencing revealed that a pectin-enriched diet (PEC) decreased the abundances of fungal keystone taxa (e.g., amino acid-producing Kazachstania spp.)					
36475916	10	23	from	interactions	1776:1787	arg1	taxa					1741:1744	gut microbial taxa	1727:1744	gut microbial taxa	1727:1744	Our study focuses on effects of pectin on gut microbial taxa and functions, as well as its interactions with altered environmental features.					
36475916	10	23	from	interactions	1776:1787	arg1	functions					1750:1758	functions	1750:1758	functions	1750:1758	Our study focuses on effects of pectin on gut microbial taxa and functions, as well as its interactions with altered environmental features.					
36475916	8	24	theme	specific	1302:1309	arg1	microorganisms					1311:1324	specific microorganisms	1302:1324	specific microorganisms	1302:1324	Overall, these results demonstrate that pectin decreases the redox potential in pig hindgut to modulate microbial composition and functions, and specific microorganisms generate reducing agents in the course of pectin degradation to decrease redox potential of microbial ecosystem.					
36475916	0	25	theme	Gut	61:63	arg1	Taxa					75:78	Gut Microbial Taxa	61:78	Gut Microbial Taxa	61:78	Reduction of Redox Potential Exerts a Key Role in Modulating Gut Microbial Taxa and Function by Dietary Supplementation of Pectin in a Pig Model.					
36475916	1	26	theme	intestinal	285:294	arg1	health					296:301	intestinal health	285:301	intestinal health	285:301	Pectin exists in a vast range of plants and has a long history of acting as a functional food additive with potential prebiotic effects on intestinal health.					
36475916	4	27	theme	Methanobrevibacter	732:749	arg1	spp					751:753	methane-producing Methanobrevibacter spp	714:753	methane-producing Methanobrevibacter spp	714:753	and their genes involved in oxidative phosphorylation, while it increased the abundance of sulfate-reducing Desulfovibrio spp., and methane-producing Methanobrevibacter spp.					
36475916	3	28	dep	revealed	443:450	arg1	spp					576:578	amino acid-producing Kazachstania spp	542:578	amino acid-producing Kazachstania spp.	542:579	Here, metatranscriptome sequencing revealed that a pectin-enriched diet (PEC) decreased the abundances of fungal keystone taxa (e.g., amino acid-producing Kazachstania spp.)					
36475916	11	29	theme	redox	1971:1975	arg1	potential					1977:1985	hindgut redox potential	1963:1985	hindgut redox potential	1963:1985	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	11	29	theme	redox	1971:1975	arg1	feature					2024:2030	an important environmental feature	1997:2030	an important environmental feature that can modulate microbial communities	1997:2070	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	4	30	theme	spp.	704:707	arg1	abundance					660:668	the abundance	656:668	the abundance of sulfate-reducing Desulfovibrio spp., and methane-producing Methanobrevibacter spp	656:753	and their genes involved in oxidative phosphorylation, while it increased the abundance of sulfate-reducing Desulfovibrio spp., and methane-producing Methanobrevibacter spp.					
36475916	11	31	theme	hindgut	1963:1969	arg1	potential					1977:1985	hindgut redox potential	1963:1985	hindgut redox potential	1963:1985	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	11	31	theme	hindgut	1963:1969	arg1	feature					2024:2030	an important environmental feature	1997:2030	an important environmental feature that can modulate microbial communities	1997:2070	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	0	32	theme	Redox	13:17	arg1	Potential					19:27	Redox Potential	13:27	Redox Potential	13:27	Reduction of Redox Potential Exerts a Key Role in Modulating Gut Microbial Taxa and Function by Dietary Supplementation of Pectin in a Pig Model.					
36475916	1	33	theme	functional	224:233	arg1	food					235:238	a functional food	222:238	a functional food additive with potential prebiotic effects on intestinal health	222:301	Pectin exists in a vast range of plants and has a long history of acting as a functional food additive with potential prebiotic effects on intestinal health.					
36475916	7	34	from	degradation	1066:1076	arg1	biosynthesis					1041:1052	inosine biosynthesis	1033:1052	inosine biosynthesis	1033:1052	Fecal metagenome analysis disclosed that certain microbial taxa promoted inosine biosynthesis from pectin degradation, including Prevotella, which plays an essential role in pectin biodegradation.					
36475916	8	35	theme	degradation	1375:1385	arg1	course					1358:1363	the course	1354:1363	the course of pectin degradation	1354:1385	Overall, these results demonstrate that pectin decreases the redox potential in pig hindgut to modulate microbial composition and functions, and specific microorganisms generate reducing agents in the course of pectin degradation to decrease redox potential of microbial ecosystem.					
36475916	2	36	theme	microbial	351:359	arg1	communities					361:371	gut microbial communities	347:371	gut microbial communities	347:371	However, knowledge of how pectin regulates gut microbial communities is still insufficient and limited.					
36475916	5	37	theme	colon	759:763	arg1	microbiomes					765:775	colon microbiomes	759:775	colon microbiomes	759:775	in colon microbiomes.					
36475916	1	38	theme	additive	240:247	arg1	food					235:238	a functional food	222:238	a functional food additive with potential prebiotic effects on intestinal health	222:301	Pectin exists in a vast range of plants and has a long history of acting as a functional food additive with potential prebiotic effects on intestinal health.					
36475916	7	39	theme	essential	1116:1124	arg1	role					1126:1129	an essential role	1113:1129	an essential role	1113:1129	Fecal metagenome analysis disclosed that certain microbial taxa promoted inosine biosynthesis from pectin degradation, including Prevotella, which plays an essential role in pectin biodegradation.					
36475916	6	40	theme	redox	835:839	arg1	potential					841:849	fecal redox potential	829:849	fecal redox potential	829:849	Furthermore, we first confirmed that PEC decreased fecal redox potential in a fistula pig model, which could be supported by the enrichment of antioxidants (e.g., inosine) in feces.					
36475916	6	41	theme	fistula	856:862	arg1	model					868:872	a fistula pig model	854:872	a fistula pig model	854:872	Furthermore, we first confirmed that PEC decreased fecal redox potential in a fistula pig model, which could be supported by the enrichment of antioxidants (e.g., inosine) in feces.					
36475916	10	42	theme	gut	1727:1729	arg1	taxa					1741:1744	gut microbial taxa	1727:1744	gut microbial taxa	1727:1744	Our study focuses on effects of pectin on gut microbial taxa and functions, as well as its interactions with altered environmental features.					
36475916	0	43	theme	Key	38:40	arg1	Role					42:45	a Key Role	36:45	a Key Role	36:45	Reduction of Redox Potential Exerts a Key Role in Modulating Gut Microbial Taxa and Function by Dietary Supplementation of Pectin in a Pig Model.					
36475916	1	44	theme	potential	254:262	arg1	effects					274:280	potential prebiotic effects	254:280	potential prebiotic effects on intestinal health	254:301	Pectin exists in a vast range of plants and has a long history of acting as a functional food additive with potential prebiotic effects on intestinal health.					
36475916	9	45	theme	altered	1623:1629	arg1	conditions					1645:1654	the altered physiological conditions	1619:1654	the altered physiological conditions caused by pectin ingestions	1619:1682	IMPORTANCE Collective studies indicate that pectin degradation promotes extensive microorganisms that can be involved in pectin degradation directly or indirectly, or benefit from the altered physiological conditions caused by pectin ingestions.					
36475916	11	46	theme	important	2000:2008	arg1	potential					1977:1985	hindgut redox potential	1963:1985	hindgut redox potential	1963:1985	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	11	46	theme	important	2000:2008	arg1	feature					2024:2030	an important environmental feature	1997:2030	an important environmental feature that can modulate microbial communities	1997:2070	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	6	47	from	enrichment	907:916	arg1	feces					953:957	feces	953:957	feces	953:957	Furthermore, we first confirmed that PEC decreased fecal redox potential in a fistula pig model, which could be supported by the enrichment of antioxidants (e.g., inosine) in feces.					
36475916	10	48	theme	pectin	1717:1722	arg1	interactions					1776:1787	its interactions	1772:1787	its interactions with altered environmental features	1772:1823	Our study focuses on effects of pectin on gut microbial taxa and functions, as well as its interactions with altered environmental features.					
36475916	10	48	theme	pectin	1717:1722	arg1	effects					1706:1712	effects	1706:1712	effects of pectin on gut microbial taxa and functions	1706:1758	Our study focuses on effects of pectin on gut microbial taxa and functions, as well as its interactions with altered environmental features.					
36475916	1	49	theme	plants	179:184	arg1	range					170:174	a vast range	163:174	a vast range of plants	163:184	Pectin exists in a vast range of plants and has a long history of acting as a functional food additive with potential prebiotic effects on intestinal health.					
36475916	8	50	theme	microbial	1418:1426	arg1	ecosystem					1428:1436	microbial ecosystem	1418:1436	microbial ecosystem	1418:1436	Overall, these results demonstrate that pectin decreases the redox potential in pig hindgut to modulate microbial composition and functions, and specific microorganisms generate reducing agents in the course of pectin degradation to decrease redox potential of microbial ecosystem.					
36475916	10	51	from	effects	1706:1712	arg1	taxa					1741:1744	gut microbial taxa	1727:1744	gut microbial taxa	1727:1744	Our study focuses on effects of pectin on gut microbial taxa and functions, as well as its interactions with altered environmental features.					
36475916	10	51	from	effects	1706:1712	arg1	functions					1750:1758	functions	1750:1758	functions	1750:1758	Our study focuses on effects of pectin on gut microbial taxa and functions, as well as its interactions with altered environmental features.					
36475916	3	52	theme	pectin-enriched	459:473	arg1	diet					475:478	a pectin-enriched diet	457:478	a pectin-enriched diet (PEC)	457:484	Here, metatranscriptome sequencing revealed that a pectin-enriched diet (PEC) decreased the abundances of fungal keystone taxa (e.g., amino acid-producing Kazachstania spp.)					
36475916	3	52	theme	pectin-enriched	459:473	arg1	PEC					481:483	PEC	481:483	PEC	481:483	Here, metatranscriptome sequencing revealed that a pectin-enriched diet (PEC) decreased the abundances of fungal keystone taxa (e.g., amino acid-producing Kazachstania spp.)					
36475916	1	53	theme	long	196:199	arg1	history					201:207	a long history	194:207	a long history of acting as a functional food additive with potential prebiotic effects on intestinal health	194:301	Pectin exists in a vast range of plants and has a long history of acting as a functional food additive with potential prebiotic effects on intestinal health.					
36475916	3	54	theme	keystone	521:528	arg1	taxa					530:533	fungal keystone taxa	514:533	fungal keystone taxa	514:533	Here, metatranscriptome sequencing revealed that a pectin-enriched diet (PEC) decreased the abundances of fungal keystone taxa (e.g., amino acid-producing Kazachstania spp.)					
36475916	1	55	with	additive	240:247	arg1	effects					274:280	potential prebiotic effects	254:280	potential prebiotic effects on intestinal health	254:301	Pectin exists in a vast range of plants and has a long history of acting as a functional food additive with potential prebiotic effects on intestinal health.					
36475916	11	56	theme	colon	1887:1891	arg1	taxa					1903:1906	colon microbial taxa	1887:1906	colon microbial taxa	1887:1906	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	7	57	theme	pectin	1059:1064	arg1	degradation					1066:1076	pectin degradation	1059:1076	pectin degradation	1059:1076	Fecal metagenome analysis disclosed that certain microbial taxa promoted inosine biosynthesis from pectin degradation, including Prevotella, which plays an essential role in pectin biodegradation.					
36475916	10	58	with	effects	1706:1712	arg1	features					1816:1823	altered environmental features	1794:1823	altered environmental features	1794:1823	Our study focuses on effects of pectin on gut microbial taxa and functions, as well as its interactions with altered environmental features.					
36475916	12	59	theme	pectin	2178:2183	arg1	degradation					2185:2195	pectin degradation	2178:2195	pectin degradation	2178:2195	These results infer that there is bidirectional regulation between microbiota and redox potential during pectin degradation.					
36475916	10	60	theme	altered	1794:1800	arg1	features					1816:1823	altered environmental features	1794:1823	altered environmental features	1794:1823	Our study focuses on effects of pectin on gut microbial taxa and functions, as well as its interactions with altered environmental features.					
36475916	9	61	theme	IMPORTANCE	1439:1448	arg1	studies					1461:1467	IMPORTANCE Collective studies	1439:1467	IMPORTANCE Collective studies	1439:1467	IMPORTANCE Collective studies indicate that pectin degradation promotes extensive microorganisms that can be involved in pectin degradation directly or indirectly, or benefit from the altered physiological conditions caused by pectin ingestions.					
36475916	4	62	theme	oxidative	610:618	arg1	phosphorylation					620:634	oxidative phosphorylation	610:634	oxidative phosphorylation	610:634	and their genes involved in oxidative phosphorylation, while it increased the abundance of sulfate-reducing Desulfovibrio spp., and methane-producing Methanobrevibacter spp.					
36475916	6	63	theme	antioxidants	921:932	arg1	enrichment					907:916	the enrichment	903:916	the enrichment of antioxidants (e.g., inosine) in feces	903:957	Furthermore, we first confirmed that PEC decreased fecal redox potential in a fistula pig model, which could be supported by the enrichment of antioxidants (e.g., inosine) in feces.					
36475916	7	64	theme	microbial	1009:1017	arg1	taxa					1019:1022	certain microbial taxa	1001:1022	certain microbial taxa	1001:1022	Fecal metagenome analysis disclosed that certain microbial taxa promoted inosine biosynthesis from pectin degradation, including Prevotella, which plays an essential role in pectin biodegradation.					
36475916	8	65	theme	redox	1218:1222	arg1	potential					1224:1232	the redox potential	1214:1232	the redox potential in pig hindgut to modulate microbial composition and functions	1214:1295	Overall, these results demonstrate that pectin decreases the redox potential in pig hindgut to modulate microbial composition and functions, and specific microorganisms generate reducing agents in the course of pectin degradation to decrease redox potential of microbial ecosystem.					
36475916	3	66	dep	spp	576:578	arg1	e.g.					536:539	e.g.	536:539	e.g.	536:539	Here, metatranscriptome sequencing revealed that a pectin-enriched diet (PEC) decreased the abundances of fungal keystone taxa (e.g., amino acid-producing Kazachstania spp.)					
36475916	13	67	theme	microbial	2282:2290	arg1	ecosystem					2292:2300	the pectin-modulating gut microbial ecosystem	2256:2300	the pectin-modulating gut microbial ecosystem	2256:2300	In general, this investigation proposes new insights into the pectin-modulating gut microbial ecosystem and also provides new perspectives for targeting modulation of gut microbiota.					
36475916	11	68	theme	proreducing	1865:1875	arg1	shifts					1877:1882	pectin-induced proreducing shifts	1850:1882	pectin-induced proreducing shifts on colon microbial taxa and functions	1850:1920	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	7	69	theme	metagenome	966:975	arg1	analysis					977:984	Fecal metagenome analysis	960:984	Fecal metagenome analysis	960:984	Fecal metagenome analysis disclosed that certain microbial taxa promoted inosine biosynthesis from pectin degradation, including Prevotella, which plays an essential role in pectin biodegradation.					
36475916	12	70	theme	bidirectional	2107:2119	arg1	regulation					2121:2130	bidirectional regulation	2107:2130	bidirectional regulation between microbiota and redox potential during pectin degradation	2107:2195	These results infer that there is bidirectional regulation between microbiota and redox potential during pectin degradation.					
36475916	11	71	theme	environmental	2010:2022	arg1	potential					1977:1985	hindgut redox potential	1963:1985	hindgut redox potential	1963:1985	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	11	71	theme	environmental	2010:2022	arg1	feature					2024:2030	an important environmental feature	1997:2030	an important environmental feature that can modulate microbial communities	1997:2070	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	9	72	theme	extensive	1511:1519	arg1	microorganisms					1521:1534	extensive microorganisms	1511:1534	extensive microorganisms that can be involved in pectin degradation directly or indirectly, or benefit from the altered physiological conditions caused by pectin ingestions	1511:1682	IMPORTANCE Collective studies indicate that pectin degradation promotes extensive microorganisms that can be involved in pectin degradation directly or indirectly, or benefit from the altered physiological conditions caused by pectin ingestions.					
36475916	3	73	theme	acid-producing	548:561	arg1	spp					576:578	amino acid-producing Kazachstania spp	542:578	amino acid-producing Kazachstania spp.	542:579	Here, metatranscriptome sequencing revealed that a pectin-enriched diet (PEC) decreased the abundances of fungal keystone taxa (e.g., amino acid-producing Kazachstania spp.)					
36475916	12	74	theme	redox	2155:2159	arg1	potential					2161:2169	redox potential	2155:2169	redox potential	2155:2169	These results infer that there is bidirectional regulation between microbiota and redox potential during pectin degradation.					
36475916	13	75	theme	new	2320:2322	arg1	perspectives					2324:2335	new perspectives	2320:2335	new perspectives	2320:2335	In general, this investigation proposes new insights into the pectin-modulating gut microbial ecosystem and also provides new perspectives for targeting modulation of gut microbiota.					
36475916	6	76	dep	inosine	941:947	arg1	e.g.					935:938	e.g.	935:938	e.g.	935:938	Furthermore, we first confirmed that PEC decreased fecal redox potential in a fistula pig model, which could be supported by the enrichment of antioxidants (e.g., inosine) in feces.					
36475916	0	77	theme	Microbial	65:73	arg1	Taxa					75:78	Gut Microbial Taxa	61:78	Gut Microbial Taxa	61:78	Reduction of Redox Potential Exerts a Key Role in Modulating Gut Microbial Taxa and Function by Dietary Supplementation of Pectin in a Pig Model.					
36475916	10	78	with	interactions	1776:1787	arg1	features					1816:1823	altered environmental features	1794:1823	altered environmental features	1794:1823	Our study focuses on effects of pectin on gut microbial taxa and functions, as well as its interactions with altered environmental features.					
36475916	0	79	theme	Dietary	96:102	arg1	Supplementation					104:118	Dietary Supplementation	96:118	Dietary Supplementation of Pectin in a Pig Model	96:143	Reduction of Redox Potential Exerts a Key Role in Modulating Gut Microbial Taxa and Function by Dietary Supplementation of Pectin in a Pig Model.					
36475916	8	80	theme	redox	1399:1403	arg1	potential					1405:1413	redox potential	1399:1413	redox potential of microbial ecosystem	1399:1436	Overall, these results demonstrate that pectin decreases the redox potential in pig hindgut to modulate microbial composition and functions, and specific microorganisms generate reducing agents in the course of pectin degradation to decrease redox potential of microbial ecosystem.					
36475916	9	81	theme	pectin	1560:1565	arg1	degradation					1567:1577	pectin degradation	1560:1577	pectin degradation	1560:1577	IMPORTANCE Collective studies indicate that pectin degradation promotes extensive microorganisms that can be involved in pectin degradation directly or indirectly, or benefit from the altered physiological conditions caused by pectin ingestions.					
36475916	4	82	theme	methane-producing	714:730	arg1	spp					751:753	methane-producing Methanobrevibacter spp	714:753	methane-producing Methanobrevibacter spp	714:753	and their genes involved in oxidative phosphorylation, while it increased the abundance of sulfate-reducing Desulfovibrio spp., and methane-producing Methanobrevibacter spp.					
36475916	11	83	from	shifts	1877:1882	arg1	functions					1912:1920	functions	1912:1920	functions	1912:1920	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	11	83	from	shifts	1877:1882	arg1	taxa					1903:1906	colon microbial taxa	1887:1906	colon microbial taxa	1887:1906	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	8	84	theme	reducing	1335:1342	arg1	agents					1344:1349	reducing agents	1335:1349	reducing agents	1335:1349	Overall, these results demonstrate that pectin decreases the redox potential in pig hindgut to modulate microbial composition and functions, and specific microorganisms generate reducing agents in the course of pectin degradation to decrease redox potential of microbial ecosystem.					
36475916	7	85	theme	pectin	1134:1139	arg1	biodegradation					1141:1154	pectin biodegradation	1134:1154	pectin biodegradation	1134:1154	Fecal metagenome analysis disclosed that certain microbial taxa promoted inosine biosynthesis from pectin degradation, including Prevotella, which plays an essential role in pectin biodegradation.					
36475916	0	86	theme	Pectin	123:128	arg1	Supplementation					104:118	Dietary Supplementation	96:118	Dietary Supplementation of Pectin in a Pig Model	96:143	Reduction of Redox Potential Exerts a Key Role in Modulating Gut Microbial Taxa and Function by Dietary Supplementation of Pectin in a Pig Model.					
36475916	4	87	theme	Desulfovibrio	690:702	arg1	spp.					704:707	sulfate-reducing Desulfovibrio spp.	673:707	sulfate-reducing Desulfovibrio spp.	673:707	and their genes involved in oxidative phosphorylation, while it increased the abundance of sulfate-reducing Desulfovibrio spp., and methane-producing Methanobrevibacter spp.					
36475916	0	88	theme	Potential	19:27	arg1	Reduction					0:8	Reduction	0:8	Reduction of Redox Potential	0:27	Reduction of Redox Potential Exerts a Key Role in Modulating Gut Microbial Taxa and Function by Dietary Supplementation of Pectin in a Pig Model.					
36475916	3	89	theme	metatranscriptome	414:430	arg1	sequencing					432:441	metatranscriptome sequencing	414:441	metatranscriptome sequencing	414:441	Here, metatranscriptome sequencing revealed that a pectin-enriched diet (PEC) decreased the abundances of fungal keystone taxa (e.g., amino acid-producing Kazachstania spp.)					
36475916	8	90	theme	pectin	1368:1373	arg1	degradation					1375:1385	pectin degradation	1368:1385	pectin degradation	1368:1385	Overall, these results demonstrate that pectin decreases the redox potential in pig hindgut to modulate microbial composition and functions, and specific microorganisms generate reducing agents in the course of pectin degradation to decrease redox potential of microbial ecosystem.					
36475916	2	91	theme	gut	347:349	arg1	communities					361:371	gut microbial communities	347:371	gut microbial communities	347:371	However, knowledge of how pectin regulates gut microbial communities is still insufficient and limited.					
36475916	1	92	from	effects	274:280	arg1	health					296:301	intestinal health	285:301	intestinal health	285:301	Pectin exists in a vast range of plants and has a long history of acting as a functional food additive with potential prebiotic effects on intestinal health.					
36475916	13	93	theme	pectin-modulating	2260:2276	arg1	ecosystem					2292:2300	the pectin-modulating gut microbial ecosystem	2256:2300	the pectin-modulating gut microbial ecosystem	2256:2300	In general, this investigation proposes new insights into the pectin-modulating gut microbial ecosystem and also provides new perspectives for targeting modulation of gut microbiota.					
36475916	6	94	theme	fecal	829:833	arg1	potential					841:849	fecal redox potential	829:849	fecal redox potential	829:849	Furthermore, we first confirmed that PEC decreased fecal redox potential in a fistula pig model, which could be supported by the enrichment of antioxidants (e.g., inosine) in feces.					
36475916	11	95	theme	microbial	1893:1901	arg1	taxa					1903:1906	colon microbial taxa	1887:1906	colon microbial taxa	1887:1906	Our results demonstrate pectin-induced proreducing shifts on colon microbial taxa and functions, and first confirm that pectin decreases hindgut redox potential, which is an important environmental feature that can modulate microbial communities.					
36475916	1	96	theme	prebiotic	264:272	arg1	effects					274:280	potential prebiotic effects	254:280	potential prebiotic effects on intestinal health	254:301	Pectin exists in a vast range of plants and has a long history of acting as a functional food additive with potential prebiotic effects on intestinal health.					
36475916	9	97	theme	physiological	1631:1643	arg1	conditions					1645:1654	the altered physiological conditions	1619:1654	the altered physiological conditions caused by pectin ingestions	1619:1682	IMPORTANCE Collective studies indicate that pectin degradation promotes extensive microorganisms that can be involved in pectin degradation directly or indirectly, or benefit from the altered physiological conditions caused by pectin ingestions.					
36475916	3	98	theme	amino	542:546	arg1	spp					576:578	amino acid-producing Kazachstania spp	542:578	amino acid-producing Kazachstania spp.	542:579	Here, metatranscriptome sequencing revealed that a pectin-enriched diet (PEC) decreased the abundances of fungal keystone taxa (e.g., amino acid-producing Kazachstania spp.)					
36475916	4	99	theme	spp	751:753	arg1	abundance					660:668	the abundance	656:668	the abundance of sulfate-reducing Desulfovibrio spp., and methane-producing Methanobrevibacter spp	656:753	and their genes involved in oxidative phosphorylation, while it increased the abundance of sulfate-reducing Desulfovibrio spp., and methane-producing Methanobrevibacter spp.					
36475916	7	100	theme	Fecal	960:964	arg1	analysis					977:984	Fecal metagenome analysis	960:984	Fecal metagenome analysis	960:984	Fecal metagenome analysis disclosed that certain microbial taxa promoted inosine biosynthesis from pectin degradation, including Prevotella, which plays an essential role in pectin biodegradation.					
36475916	13	101	theme	gut	2278:2280	arg1	ecosystem					2292:2300	the pectin-modulating gut microbial ecosystem	2256:2300	the pectin-modulating gut microbial ecosystem	2256:2300	In general, this investigation proposes new insights into the pectin-modulating gut microbial ecosystem and also provides new perspectives for targeting modulation of gut microbiota.					
36475916	13	102	theme	new	2238:2240	arg1	insights					2242:2249	new insights	2238:2249	new insights into the pectin-modulating gut microbial ecosystem	2238:2300	In general, this investigation proposes new insights into the pectin-modulating gut microbial ecosystem and also provides new perspectives for targeting modulation of gut microbiota.					
36475916	0	103	from	Supplementation	104:118	arg1	Model					139:143	a Pig Model	133:143	a Pig Model	133:143	Reduction of Redox Potential Exerts a Key Role in Modulating Gut Microbial Taxa and Function by Dietary Supplementation of Pectin in a Pig Model.					
35726720	5	0	theme	SF	1034:1035	arg1	hydrogel					1040:1047	The COL + SF(S) hydrogel	1024:1047	The COL + SF(S) hydrogel	1024:1047	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	3	1	theme	silk-collagen	732:744	arg1	hydrogel					756:763	a non-sonicated silk-collagen composite hydrogel	716:763	a non-sonicated silk-collagen composite hydrogel (COL + SF(NS))	716:778	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	1	2	theme	fibrocartilage	185:198	arg1	formation					200:208	fibrocartilage formation	185:208	fibrocartilage formation	185:208	Cartilage tissue has limited self-regeneration capacity and current treatment methods often result in fibrocartilage formation.					
35726720	7	3	theme	articular	1401:1409	arg1	cartilage					1419:1427	well-integrated articular hyaline cartilage	1385:1427	well-integrated articular hyaline cartilage closely resembling native articular cartilage	1385:1473	The COL + SF(S) group exhibited well-integrated articular hyaline cartilage closely resembling native articular cartilage after 6 months.					
35726720	6	4	theme	COL	1297:1299	arg1	group					1309:1313	the COL + SF(S) group	1293:1313	the COL + SF(S) group	1293:1313	Moreover, the cartilage regeneration capacity of the COL + SF(S) group was evaluated in rabbit knee defects.					
35726720	7	5	theme	articular	1455:1463	arg1	cartilage					1465:1473	native articular cartilage	1448:1473	native articular cartilage	1448:1473	The COL + SF(S) group exhibited well-integrated articular hyaline cartilage closely resembling native articular cartilage after 6 months.					
35726720	2	6	theme	hydrogel	378:385	arg1	application					347:357	the application	343:357	the application of a pure collagen hydrogel	343:385	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	3	7	dep	hydrogel	586:593	arg1	COL					596:598	COL	596:598	COL	596:598	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	3	7	dep	hydrogel	586:593	arg1	S					605:605	S	605:605	S	605:605	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	3	7	dep	hydrogel	586:593	arg1	SF					602:603	SF	602:603	SF	602:603	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	4	8	from	formation	875:883	arg1	molecule					936:943	the silk fibroin molecule	919:943	the silk fibroin molecule	919:943	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	4	9	theme	stronger	891:898	arg1	charge					909:914	a stronger negative charge	889:914	a stronger negative charge on the silk fibroin molecule	889:943	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	2	10	theme	hyaline	324:330	arg1	cartilage					332:340	hyaline cartilage	324:340	hyaline cartilage	324:340	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	4	11	dep	induced	846:852	arg1	resulted					952:959	resulted	952:959	resulted in improved mechanical properties of the COL + SF(S) hydrogel	952:1021	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	5	12	theme	sulfated	1162:1169	arg1	deposition					1196:1205	sulfated glycosaminoglycan (sGAG) deposition	1162:1205	sulfated glycosaminoglycan (sGAG) deposition	1162:1205	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	7	13	theme	SF	1363:1364	arg1	group					1369:1373	The COL + SF(S) group	1353:1373	group	1369:1373	The COL + SF(S) group exhibited well-integrated articular hyaline cartilage closely resembling native articular cartilage after 6 months.					
35726720	7	13	theme	SF	1363:1364	arg1	S					1366:1366	S	1366:1366	S	1366:1366	The COL + SF(S) group exhibited well-integrated articular hyaline cartilage closely resembling native articular cartilage after 6 months.					
35726720	1	14	theme	Cartilage	83:91	arg1	tissue					93:98	Cartilage tissue	83:98	Cartilage tissue	83:98	Cartilage tissue has limited self-regeneration capacity and current treatment methods often result in fibrocartilage formation.					
35726720	5	15	theme	glycosaminoglycan	1171:1187	arg1	deposition					1196:1205	sulfated glycosaminoglycan (sGAG) deposition	1162:1205	sulfated glycosaminoglycan (sGAG) deposition	1162:1205	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	4	16	theme	improved	964:971	arg1	properties					984:993	improved mechanical properties	964:993	improved mechanical properties of the COL + SF(S) hydrogel	964:1021	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	2	17	theme	collagen	369:376	arg1	hydrogel					378:385	a pure collagen hydrogel	362:385	a pure collagen hydrogel	362:385	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	6	18	theme	regeneration	1268:1279	arg1	capacity					1281:1288	the cartilage regeneration capacity	1254:1288	the cartilage regeneration capacity of the COL + SF(S) group	1254:1313	Moreover, the cartilage regeneration capacity of the COL + SF(S) group was evaluated in rabbit knee defects.					
35726720	4	19	theme	SF	1008:1009	arg1	hydrogel					1014:1021	SF(S) hydrogel	1008:1021	SF(S) hydrogel	1008:1021	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	3	20	theme	composite	746:754	arg1	hydrogel					756:763	a non-sonicated silk-collagen composite hydrogel	716:763	a non-sonicated silk-collagen composite hydrogel (COL + SF(NS))	716:778	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	4	21	theme	fibroin	838:844	arg1	treatment					820:828	the sonication treatment	805:828	the sonication treatment of silk fibroin	805:844	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	4	22	theme	S	1011:1011	arg1	hydrogel					1014:1021	SF(S) hydrogel	1008:1021	SF(S) hydrogel	1008:1021	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	2	23	theme	fast	416:419	arg1	degradation					421:431	its fast degradation	412:431	its fast degradation	412:431	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	0	24	theme	sonication-induced	2:19	arg1	hydrogel					35:42	A sonication-induced silk-collagen hydrogel	0:42	A sonication-induced silk-collagen hydrogel for functional cartilage regeneration	0:80	A sonication-induced silk-collagen hydrogel for functional cartilage regeneration.					
35726720	5	25	theme	early	1146:1150	arg1	stage					1152:1156	the early stage	1142:1156	the early stage	1142:1156	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	4	26	theme	β-sheet	867:873	arg1	formation					875:883	antiparallel β-sheet formation	854:883	antiparallel β-sheet formation	854:883	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	5	27	theme	SOX9	1134:1137	arg1	expression					1120:1129	the gene expression	1111:1129	the gene expression of SOX9 at the early stage	1111:1156	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	5	27	theme	SOX9	1134:1137	arg1	deposition					1196:1205	sulfated glycosaminoglycan (sGAG) deposition	1162:1205	sulfated glycosaminoglycan (sGAG) deposition	1162:1205	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	1	28	theme	limited	104:110	arg1	capacity					130:137	limited self-regeneration capacity	104:137	limited self-regeneration capacity	104:137	Cartilage tissue has limited self-regeneration capacity and current treatment methods often result in fibrocartilage formation.					
35726720	5	29	theme	superior	1059:1066	arg1	stability					1068:1076	superior stability	1059:1076	superior stability	1059:1076	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	2	30	theme	cell-mediated	475:487	arg1	shrinkage					489:497	excessive cell-mediated shrinkage	465:497	excessive cell-mediated shrinkage	465:497	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	1	31	contain	has	100:102	arg1	tissue					93:98	Cartilage tissue	83:98	Cartilage tissue	83:98	Cartilage tissue has limited self-regeneration capacity and current treatment methods often result in fibrocartilage formation.					
35726720	1	31	contain	has	100:102	arg2	capacity					130:137	limited self-regeneration capacity	104:137	limited self-regeneration capacity	104:137	Cartilage tissue has limited self-regeneration capacity and current treatment methods often result in fibrocartilage formation.					
35726720	4	32	theme	antiparallel	854:865	arg1	formation					875:883	antiparallel β-sheet formation	854:883	antiparallel β-sheet formation	854:883	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	7	33	theme	hyaline	1411:1417	arg1	cartilage					1419:1427	well-integrated articular hyaline cartilage	1385:1427	well-integrated articular hyaline cartilage closely resembling native articular cartilage	1385:1473	The COL + SF(S) group exhibited well-integrated articular hyaline cartilage closely resembling native articular cartilage after 6 months.					
35726720	1	34	theme	self-regeneration	112:128	arg1	capacity					130:137	limited self-regeneration capacity	104:137	limited self-regeneration capacity	104:137	Cartilage tissue has limited self-regeneration capacity and current treatment methods often result in fibrocartilage formation.					
35726720	5	35	theme	S	1037:1037	arg1	hydrogel					1040:1047	The COL + SF(S) hydrogel	1024:1047	The COL + SF(S) hydrogel	1024:1047	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	2	36	theme	excessive	465:473	arg1	shrinkage					489:497	excessive cell-mediated shrinkage	465:497	excessive cell-mediated shrinkage	465:497	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	3	37	theme	physicochemical	630:644	arg1	properties					661:670	its physicochemical and biological properties	626:670	its physicochemical and biological properties	626:670	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	4	38	theme	fibroin	928:934	arg1	molecule					936:943	the silk fibroin molecule	919:943	the silk fibroin molecule	919:943	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	6	39	theme	group	1309:1313	arg1	capacity					1281:1288	the cartilage regeneration capacity	1254:1288	the cartilage regeneration capacity of the COL + SF(S) group	1254:1313	Moreover, the cartilage regeneration capacity of the COL + SF(S) group was evaluated in rabbit knee defects.					
35726720	0	40	theme	silk-collagen	21:33	arg1	hydrogel					35:42	A sonication-induced silk-collagen hydrogel	0:42	A sonication-induced silk-collagen hydrogel for functional cartilage regeneration	0:80	A sonication-induced silk-collagen hydrogel for functional cartilage regeneration.					
35726720	4	41	theme	silk	923:926	arg1	molecule					936:943	the silk fibroin molecule	919:943	the silk fibroin molecule	919:943	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	3	42	theme	sonication-induced	543:560	arg1	hydrogel					586:593	a sonication-induced silk-collagen composite hydrogel	541:593	a sonication-induced silk-collagen composite hydrogel (COL + SF(S))	541:607	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	5	43	theme	exogenous	1219:1227	arg1	factor					1236:1241	any exogenous growth factor	1215:1241	any exogenous growth factor	1215:1241	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	2	44	theme	mechanical	439:448	arg1	properties					450:459	poor mechanical properties	434:459	poor mechanical properties	434:459	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	0	45	theme	functional	48:57	arg1	regeneration					69:80	functional cartilage regeneration	48:80	functional cartilage regeneration	48:80	A sonication-induced silk-collagen hydrogel for functional cartilage regeneration.					
35726720	2	46	theme	pure	364:367	arg1	hydrogel					378:385	a pure collagen hydrogel	362:385	a pure collagen hydrogel	362:385	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	5	47	theme	cell	1085:1088	arg1	culture					1090:1096	cell culture	1085:1096	cell culture	1085:1096	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	3	48	theme	collagen	688:695	arg1	COL					707:709	COL	707:709	COL	707:709	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	3	48	theme	collagen	688:695	arg1	hydrogel					697:704	a collagen hydrogel	686:704	a collagen hydrogel (COL)	686:710	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	2	49	theme	mesenchymal	279:289	arg1	cells					296:300	mesenchymal stem cells	279:300	mesenchymal stem cells (MSCs)	279:307	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	2	49	theme	mesenchymal	279:289	arg1	MSCs					303:306	MSCs	303:306	MSCs	303:306	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	7	50	theme	native	1448:1453	arg1	cartilage					1465:1473	native articular cartilage	1448:1473	native articular cartilage	1448:1473	The COL + SF(S) group exhibited well-integrated articular hyaline cartilage closely resembling native articular cartilage after 6 months.					
35726720	2	51	theme	poor	434:437	arg1	properties					450:459	poor mechanical properties	434:459	poor mechanical properties	434:459	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	4	52	theme	COL	1002:1004	arg1	properties					984:993	improved mechanical properties	964:993	improved mechanical properties of the COL + SF(S) hydrogel	964:1021	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	8	53	theme	COL	1504:1506	arg1	S					1513:1513	the COL + SF(S)	1500:1514	the COL + SF(S) hydrogel	1500:1523	Overall, the COL + SF(S) hydrogel holds great potential as a scaffold material to regenerate functional hyaline cartilage.					
35726720	5	54	from	stage	1152:1156	arg1	expression					1120:1129	the gene expression	1111:1129	the gene expression of SOX9 at the early stage	1111:1156	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	5	54	from	stage	1152:1156	arg1	deposition					1196:1205	sulfated glycosaminoglycan (sGAG) deposition	1162:1205	sulfated glycosaminoglycan (sGAG) deposition	1162:1205	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	4	55	theme	sonication	809:818	arg1	treatment					820:828	the sonication treatment	805:828	the sonication treatment of silk fibroin	805:844	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	6	56	theme	SF	1303:1304	arg1	group					1309:1313	the COL + SF(S) group	1293:1313	the COL + SF(S) group	1293:1313	Moreover, the cartilage regeneration capacity of the COL + SF(S) group was evaluated in rabbit knee defects.					
35726720	6	57	theme	knee	1339:1342	arg1	defects					1344:1350	rabbit knee defects	1332:1350	rabbit knee defects	1332:1350	Moreover, the cartilage regeneration capacity of the COL + SF(S) group was evaluated in rabbit knee defects.					
35726720	3	58	dep	hydrogel	756:763	arg1	NS					775:776	NS	775:776	NS	775:776	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	3	58	dep	hydrogel	756:763	arg1	SF					772:773	SF	772:773	SF	772:773	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	3	58	dep	hydrogel	756:763	arg1	COL					766:768	COL	766:768	COL	766:768	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	4	59	theme	silk	833:836	arg1	fibroin					838:844	silk fibroin	833:844	silk fibroin	833:844	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	1	60	theme	current	143:149	arg1	methods					161:167	current treatment methods	143:167	current treatment methods	143:167	Cartilage tissue has limited self-regeneration capacity and current treatment methods often result in fibrocartilage formation.					
35726720	0	61	theme	cartilage	59:67	arg1	regeneration					69:80	functional cartilage regeneration	48:80	functional cartilage regeneration	48:80	A sonication-induced silk-collagen hydrogel for functional cartilage regeneration.					
35726720	8	62	theme	SF	1510:1511	arg1	S					1513:1513	the COL + SF(S)	1500:1514	the COL + SF(S) hydrogel	1500:1523	Overall, the COL + SF(S) hydrogel holds great potential as a scaffold material to regenerate functional hyaline cartilage.					
35726720	4	63	theme	negative	900:907	arg1	charge					909:914	a stronger negative charge	889:914	a stronger negative charge on the silk fibroin molecule	889:943	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	5	64	theme	COL	1028:1030	arg1	hydrogel					1040:1047	The COL + SF(S) hydrogel	1024:1047	The COL + SF(S) hydrogel	1024:1047	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	1	65	theme	treatment	151:159	arg1	methods					161:167	current treatment methods	143:167	current treatment methods	143:167	Cartilage tissue has limited self-regeneration capacity and current treatment methods often result in fibrocartilage formation.					
35726720	8	66	theme	scaffold	1552:1559	arg1	material					1561:1568	a scaffold material	1550:1568	a scaffold material	1550:1568	Overall, the COL + SF(S) hydrogel holds great potential as a scaffold material to regenerate functional hyaline cartilage.					
35726720	6	67	theme	cartilage	1258:1266	arg1	capacity					1281:1288	the cartilage regeneration capacity	1254:1288	the cartilage regeneration capacity of the COL + SF(S) group	1254:1313	Moreover, the cartilage regeneration capacity of the COL + SF(S) group was evaluated in rabbit knee defects.					
35726720	4	68	theme	hydrogel	1014:1021	arg1	properties					984:993	improved mechanical properties	964:993	improved mechanical properties of the COL + SF(S) hydrogel	964:1021	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	3	69	theme	biological	650:659	arg1	properties					661:670	its physicochemical and biological properties	626:670	its physicochemical and biological properties	626:670	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	7	70	theme	well-integrated	1385:1399	arg1	cartilage					1419:1427	well-integrated articular hyaline cartilage	1385:1427	well-integrated articular hyaline cartilage closely resembling native articular cartilage	1385:1473	The COL + SF(S) group exhibited well-integrated articular hyaline cartilage closely resembling native articular cartilage after 6 months.					
35726720	8	71	theme	S	1513:1513	arg1	hydrogel					1516:1523	the COL + SF(S) hydrogel	1500:1523	the COL + SF(S) hydrogel	1500:1523	Overall, the COL + SF(S) hydrogel holds great potential as a scaffold material to regenerate functional hyaline cartilage.					
35726720	4	72	from	charge	909:914	arg1	molecule					936:943	the silk fibroin molecule	919:943	the silk fibroin molecule	919:943	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	5	73	theme	growth	1229:1234	arg1	factor					1236:1241	any exogenous growth factor	1215:1241	any exogenous growth factor	1215:1241	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
35726720	2	74	theme	cells	296:300	arg1	chondrogenesis					261:274	chondrogenesis	261:274	chondrogenesis of mesenchymal stem cells (MSCs)	261:307	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	8	75	theme	functional	1584:1593	arg1	cartilage					1603:1611	functional hyaline cartilage	1584:1611	functional hyaline cartilage	1584:1611	Overall, the COL + SF(S) hydrogel holds great potential as a scaffold material to regenerate functional hyaline cartilage.					
35726720	3	76	theme	silk-collagen	562:574	arg1	hydrogel					586:593	a sonication-induced silk-collagen composite hydrogel	541:593	a sonication-induced silk-collagen composite hydrogel (COL + SF(S))	541:607	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	8	77	theme	hyaline	1595:1601	arg1	cartilage					1603:1611	functional hyaline cartilage	1584:1611	functional hyaline cartilage	1584:1611	Overall, the COL + SF(S) hydrogel holds great potential as a scaffold material to regenerate functional hyaline cartilage.					
35726720	4	78	theme	mechanical	973:982	arg1	properties					984:993	improved mechanical properties	964:993	improved mechanical properties of the COL + SF(S) hydrogel	964:1021	The results showed that the sonication treatment of silk fibroin induced antiparallel β-sheet formation and a stronger negative charge on the silk fibroin molecule, which resulted in improved mechanical properties of the COL + SF(S) hydrogel.					
35726720	3	79	theme	non-sonicated	718:730	arg1	hydrogel					756:763	a non-sonicated silk-collagen composite hydrogel	716:763	a non-sonicated silk-collagen composite hydrogel (COL + SF(NS))	716:778	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	2	80	theme	stem	291:294	arg1	cells					296:300	mesenchymal stem cells	279:300	mesenchymal stem cells (MSCs)	279:307	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	2	80	theme	stem	291:294	arg1	MSCs					303:306	MSCs	303:306	MSCs	303:306	Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-mediated shrinkage.					
35726720	6	81	theme	rabbit	1332:1337	arg1	defects					1344:1350	rabbit knee defects	1332:1350	rabbit knee defects	1332:1350	Moreover, the cartilage regeneration capacity of the COL + SF(S) group was evaluated in rabbit knee defects.					
35726720	3	82	theme	composite	576:584	arg1	hydrogel					586:593	a sonication-induced silk-collagen composite hydrogel	541:593	a sonication-induced silk-collagen composite hydrogel (COL + SF(S))	541:607	To overcome this challenge, we developed a sonication-induced silk-collagen composite hydrogel (COL + SF(S)) and investigated its physicochemical and biological properties compared with a collagen hydrogel (COL) and a non-sonicated silk-collagen composite hydrogel (COL + SF(NS)).					
35726720	8	83	theme	great	1531:1535	arg1	potential					1537:1545	great potential	1531:1545	great potential as a scaffold material to regenerate functional hyaline cartilage	1531:1611	Overall, the COL + SF(S) hydrogel holds great potential as a scaffold material to regenerate functional hyaline cartilage.					
35726720	5	84	theme	gene	1115:1118	arg1	expression					1120:1129	the gene expression	1111:1129	the gene expression of SOX9 at the early stage	1111:1156	The COL + SF(S) hydrogel exhibited superior stability during cell culture and promoted the gene expression of SOX9 at the early stage and sulfated glycosaminoglycan (sGAG) deposition without any exogenous growth factor.					
36515220	8	0	theme	cryoprotectants	1288:1302	arg1	type					1266:1269	type	1266:1269	type	1266:1269	There was no difference in viability, regardless of the reduction in the concentration and type of intracellular cryoprotectants and sucrose.					
36515220	8	0	theme	cryoprotectants	1288:1302	arg1	concentration					1248:1260	concentration	1248:1260	concentration	1248:1260	There was no difference in viability, regardless of the reduction in the concentration and type of intracellular cryoprotectants and sucrose.					
36515220	10	1	theme	DMSO	1515:1518	arg1	concentration					1498:1510	the concentration	1494:1510	the concentration of DMSO or EG	1494:1524	In summary, we demonstrate that reducing the concentration of DMSO or EG ensures adequate cryopreservation of Puma somatic cells, regardless of the presence of SUC.					
36515220	2	2	theme	importance	323:332	arg1	felids					302:307	Pumas, wild felids	290:307	felids	302:307	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	4	3	theme	cryoprotectants	571:585	arg1	types					548:552	types	548:552	types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC)	548:672	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	4	3	theme	cryoprotectants	571:585	arg1	sulfoxide					597:605	dimethyl sulfoxide	588:605	dimethyl sulfoxide	588:605	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	4	3	theme	cryoprotectants	571:585	arg1	concentrations					529:542	different concentrations	519:542	different concentrations	519:542	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	6	4	theme	morphological	987:999	arg1	characteristics					1001:1015	morphological characteristics	987:1015	morphological characteristics	987:1015	The cells were analyzed for morphological characteristics, viability, proliferative activity, metabolic activity, and apoptosis levels.					
36515220	2	5	theme	ecological	312:321	arg1	importance					323:332	ecological importance	312:332	ecological importance	312:332	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	4	6	theme	intracellular	557:569	arg1	cryoprotectants					571:585	intracellular cryoprotectants	557:585	intracellular cryoprotectants	557:585	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	5	7	with	medium	847:852	arg1	serum					876:880	10% fetal bovine serum	859:880	10% fetal bovine serum	859:880	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	5	7	with	medium	847:852	arg1	concentrations					894:907	varying concentrations	886:907	varying concentrations of DMSO and EG	886:922	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	4	8	theme	captive	722:728	arg1	Pumas					730:734	captive Pumas	722:734	captive Pumas	722:734	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	5	9	theme	DMSO	912:915	arg1	serum					876:880	10% fetal bovine serum	859:880	10% fetal bovine serum	859:880	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	5	9	theme	DMSO	912:915	arg1	concentrations					894:907	varying concentrations	886:907	varying concentrations of DMSO and EG	886:922	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	4	10	theme	dimethyl	588:595	arg1	types					548:552	types	548:552	types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC)	548:672	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	4	10	theme	dimethyl	588:595	arg1	DMSO					608:611	DMSO	608:611	DMSO	608:611	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	4	10	theme	dimethyl	588:595	arg1	sulfoxide					597:605	dimethyl sulfoxide	588:605	dimethyl sulfoxide	588:605	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	0	11	theme	sucrose	95:101	arg1	concentration					19:31	concentration	19:31	concentration	19:31	Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas.					
36515220	0	11	theme	sucrose	95:101	arg1	presence					83:90	the presence	79:90	the presence of sucrose	79:101	Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas.					
36515220	0	11	theme	sucrose	95:101	arg1	type					37:40	type	37:40	type	37:40	Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas.					
36515220	5	12	theme	EG	921:922	arg1	serum					876:880	10% fetal bovine serum	859:880	10% fetal bovine serum	859:880	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	5	12	theme	EG	921:922	arg1	concentrations					894:907	varying concentrations	886:907	varying concentrations of DMSO and EG	886:922	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	5	13	theme	10	859:860	arg1	%					861:861	%	861:861	%	861:861	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	1	14	theme	somatic	252:258	arg1	cells					260:264	somatic cells	252:264	somatic cells	252:264	The loss of wild biodiversity has prompted the development of cryobanks, such as those of somatic cells.					
36515220	10	15	theme	adequate	1534:1541	arg1	cryopreservation					1543:1558	adequate cryopreservation	1534:1558	adequate cryopreservation of Puma somatic cells	1534:1580	In summary, we demonstrate that reducing the concentration of DMSO or EG ensures adequate cryopreservation of Puma somatic cells, regardless of the presence of SUC.					
36515220	6	16	theme	apoptosis	1077:1085	arg1	levels					1087:1092	apoptosis levels	1077:1092	apoptosis levels	1077:1092	The cells were analyzed for morphological characteristics, viability, proliferative activity, metabolic activity, and apoptosis levels.					
36515220	4	17	theme	ethylene	614:621	arg1	glycol					623:628	ethylene glycol	614:628	ethylene glycol	614:628	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	2	18	theme	anthropogenic	351:363	arg1	actions					365:371	anthropogenic actions	351:371	anthropogenic actions	351:371	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	2	19	theme	diversity	425:433	arg1	actions					365:371	anthropogenic actions	351:371	anthropogenic actions	351:371	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	2	19	theme	diversity	425:433	arg1	decline					385:391	population decline	374:391	population decline	374:391	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	2	19	theme	diversity	425:433	arg1	loss					409:412	subsequent loss	398:412	subsequent loss of genetic diversity	398:433	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	5	20	theme	%	861:861	arg1	serum					876:880	10% fetal bovine serum	859:880	10% fetal bovine serum	859:880	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	4	21	theme	Pumas	730:734	arg1	cells					713:717	the somatic cells	701:717	the somatic cells of captive Pumas	701:734	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	0	22	attach	derived	134:140	arg1	Pumas					155:159	captive Pumas	147:159	captive Pumas	147:159	Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas.					
36515220	0	22	attach	derived	134:140	arg2	cells					128:132	somatic cells	120:132	somatic cells derived from captive Pumas	120:159	Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas.					
36515220	5	23	theme	modified	830:837	arg1	medium					847:852	Dulbecco's modified Eagle's medium	819:852	Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG	819:922	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	2	24	theme	genetic	417:423	arg1	diversity					425:433	genetic diversity	417:433	genetic diversity	417:433	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	10	25	theme	cells	1576:1580	arg1	cryopreservation					1543:1558	adequate cryopreservation	1534:1558	adequate cryopreservation of Puma somatic cells	1534:1580	In summary, we demonstrate that reducing the concentration of DMSO or EG ensures adequate cryopreservation of Puma somatic cells, regardless of the presence of SUC.					
36515220	5	26	contain	containing	808:817	arg2	medium					847:852	Dulbecco's modified Eagle's medium	819:852	Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG	819:922	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	5	26	contain	containing	808:817	arg1	solutions					798:806	different solutions	788:806	different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC	788:956	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	2	27	theme	Pumas	290:294	arg1	felids					302:307	Pumas, wild felids	290:307	felids	302:307	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	6	28	theme	proliferative	1029:1041	arg1	activity					1043:1050	proliferative activity	1029:1050	proliferative activity	1029:1050	The cells were analyzed for morphological characteristics, viability, proliferative activity, metabolic activity, and apoptosis levels.					
36515220	1	29	theme	wild	174:177	arg1	biodiversity					179:190	wild biodiversity	174:190	wild biodiversity	174:190	The loss of wild biodiversity has prompted the development of cryobanks, such as those of somatic cells.					
36515220	5	30	theme	slow	769:772	arg1	freezing					774:781	slow freezing	769:781	slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC	769:956	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	1	31	theme	biodiversity	179:190	arg1	loss					166:169	The loss	162:169	The loss of wild biodiversity	162:190	The loss of wild biodiversity has prompted the development of cryobanks, such as those of somatic cells.					
36515220	7	32	theme	similar	1112:1118	arg1	morphology					1129:1138	similar fusiform morphology	1112:1138	similar fusiform morphology	1112:1138	Cells maintained similar fusiform morphology before and after cryopreservation.					
36515220	0	33	theme	cells	128:132	arg1	cryobanks					107:115	cryobanks	107:115	cryobanks of somatic cells derived from captive Pumas	107:159	Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas.					
36515220	10	34	theme	SUC	1613:1615	arg1	presence					1601:1608	the presence	1597:1608	the presence of SUC	1597:1615	In summary, we demonstrate that reducing the concentration of DMSO or EG ensures adequate cryopreservation of Puma somatic cells, regardless of the presence of SUC.					
36515220	4	35	theme	cells	713:717	arg1	cryopreservation					681:696	the cryopreservation	677:696	the cryopreservation of the somatic cells of captive Pumas	677:734	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	3	36	theme	Somatic	436:442	arg1	banks					449:453	Somatic cell banks	436:453	Somatic cell banks	436:453	Somatic cell banks are a strategy for conserving population diversity.					
36515220	3	36	theme	Somatic	436:442	arg1	strategy					461:468	a strategy	459:468	a strategy for conserving population diversity	459:504	Somatic cell banks are a strategy for conserving population diversity.					
36515220	2	37	theme	subsequent	398:407	arg1	loss					409:412	subsequent loss	398:412	subsequent loss of genetic diversity	398:433	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	0	38	theme	somatic	120:126	arg1	cells					128:132	somatic cells	120:132	somatic cells derived from captive Pumas	120:159	Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas.					
36515220	4	39	theme	somatic	705:711	arg1	cells					713:717	the somatic cells	701:717	the somatic cells of captive Pumas	701:734	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	8	40	from	reduction	1231:1239	arg1	type					1266:1269	type	1266:1269	type	1266:1269	There was no difference in viability, regardless of the reduction in the concentration and type of intracellular cryoprotectants and sucrose.					
36515220	8	40	from	reduction	1231:1239	arg1	concentration					1248:1260	concentration	1248:1260	concentration	1248:1260	There was no difference in viability, regardless of the reduction in the concentration and type of intracellular cryoprotectants and sucrose.					
36515220	3	41	theme	cell	444:447	arg1	banks					449:453	Somatic cell banks	436:453	Somatic cell banks	436:453	Somatic cell banks are a strategy for conserving population diversity.					
36515220	3	41	theme	cell	444:447	arg1	strategy					461:468	a strategy	459:468	a strategy for conserving population diversity	459:504	Somatic cell banks are a strategy for conserving population diversity.					
36515220	5	42	theme	bovine	869:874	arg1	serum					876:880	10% fetal bovine serum	859:880	10% fetal bovine serum	859:880	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	10	43	theme	Puma	1563:1566	arg1	cells					1576:1580	Puma somatic cells	1563:1580	Puma somatic cells	1563:1580	In summary, we demonstrate that reducing the concentration of DMSO or EG ensures adequate cryopreservation of Puma somatic cells, regardless of the presence of SUC.					
36515220	5	44	theme	SUC	954:956	arg1	presence					942:949	presence	942:949	presence	942:949	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	5	44	theme	SUC	954:956	arg1	absence					931:937	absence	931:937	absence	931:937	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	5	45	dep	absence	931:937	arg1	the					927:929	the	927:929	the	927:929	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	4	46	theme	sucrose	660:666	arg1	0.2 M					651:655	0.2 M	651:655	0.2 M of sucrose (SUC)	651:672	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	9	47	theme	proliferative	1328:1340	arg1	activity					1342:1349	proliferative activity	1328:1349	proliferative activity	1328:1349	Similarly, proliferative activity, metabolic activity, and apoptosis levels were not altered by the composition of the cryoprotectants.					
36515220	5	48	theme	Eagle	839:843	arg1	medium					847:852	Dulbecco's modified Eagle's medium	819:852	Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG	819:922	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	10	49	theme	somatic	1568:1574	arg1	cells					1576:1580	Puma somatic cells	1563:1580	Puma somatic cells	1563:1580	In summary, we demonstrate that reducing the concentration of DMSO or EG ensures adequate cryopreservation of Puma somatic cells, regardless of the presence of SUC.					
36515220	2	50	theme	population	374:383	arg1	decline					385:391	population decline	374:391	population decline	374:391	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	0	51	theme	cryoprotectants	59:73	arg1	concentration					19:31	concentration	19:31	concentration	19:31	Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas.					
36515220	0	51	theme	cryoprotectants	59:73	arg1	presence					83:90	the presence	79:90	the presence of sucrose	79:101	Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas.					
36515220	0	51	theme	cryoprotectants	59:73	arg1	type					37:40	type	37:40	type	37:40	Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas.					
36515220	6	52	theme	metabolic	1053:1061	arg1	activity					1063:1070	metabolic activity	1053:1070	metabolic activity	1053:1070	The cells were analyzed for morphological characteristics, viability, proliferative activity, metabolic activity, and apoptosis levels.					
36515220	5	53	theme	fetal	863:867	arg1	serum					876:880	10% fetal bovine serum	859:880	10% fetal bovine serum	859:880	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	0	54	theme	intracellular	45:57	arg1	cryoprotectants					59:73	intracellular cryoprotectants	45:73	intracellular cryoprotectants	45:73	Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas.					
36515220	4	55	theme	different	519:527	arg1	concentrations					529:542	different concentrations	519:542	different concentrations	519:542	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	0	56	theme	captive	147:153	arg1	Pumas					155:159	captive Pumas	147:159	captive Pumas	147:159	Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas.					
36515220	9	57	theme	cryoprotectants	1436:1450	arg1	composition					1417:1427	the composition	1413:1427	the composition of the cryoprotectants	1413:1450	Similarly, proliferative activity, metabolic activity, and apoptosis levels were not altered by the composition of the cryoprotectants.					
36515220	2	58	theme	felids	302:307	arg1	This					267:270	This	267:270	This	267:270	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	2	58	theme	felids	302:307	arg1	reality					279:285	the reality	275:285	the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity	275:433	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	9	59	theme	metabolic	1352:1360	arg1	activity					1362:1369	metabolic activity	1352:1369	metabolic activity	1352:1369	Similarly, proliferative activity, metabolic activity, and apoptosis levels were not altered by the composition of the cryoprotectants.					
36515220	5	60	theme	varying	886:892	arg1	concentrations					894:907	varying concentrations	886:907	varying concentrations of DMSO and EG	886:922	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	2	61	theme	wild	297:300	arg1	felids					302:307	Pumas, wild felids	290:307	felids	302:307	This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity.					
36515220	5	62	with	freezing	774:781	arg1	solutions					798:806	different solutions	788:806	different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC	788:956	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	8	63	theme	intracellular	1274:1286	arg1	cryoprotectants					1288:1302	intracellular cryoprotectants	1274:1302	intracellular cryoprotectants	1274:1302	There was no difference in viability, regardless of the reduction in the concentration and type of intracellular cryoprotectants and sucrose.					
36515220	8	64	theme	sucrose	1308:1314	arg1	type					1266:1269	type	1266:1269	type	1266:1269	There was no difference in viability, regardless of the reduction in the concentration and type of intracellular cryoprotectants and sucrose.					
36515220	8	64	theme	sucrose	1308:1314	arg1	concentration					1248:1260	concentration	1248:1260	concentration	1248:1260	There was no difference in viability, regardless of the reduction in the concentration and type of intracellular cryoprotectants and sucrose.					
36515220	1	65	theme	cryobanks	224:232	arg1	development					209:219	the development	205:219	the development of cryobanks	205:232	The loss of wild biodiversity has prompted the development of cryobanks, such as those of somatic cells.					
36515220	10	66	theme	EG	1523:1524	arg1	concentration					1498:1510	the concentration	1494:1510	the concentration of DMSO or EG	1494:1524	In summary, we demonstrate that reducing the concentration of DMSO or EG ensures adequate cryopreservation of Puma somatic cells, regardless of the presence of SUC.					
36515220	4	67	dep	sulfoxide	597:605	arg1	EG					631:632	EG	631:632	EG	631:632	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	4	67	dep	sulfoxide	597:605	arg1	glycol					623:628	ethylene glycol	614:628	ethylene glycol	614:628	We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas.					
36515220	8	68	dep	concentration	1248:1260	arg1	the					1244:1246	the	1244:1246	the	1244:1246	There was no difference in viability, regardless of the reduction in the concentration and type of intracellular cryoprotectants and sucrose.					
36515220	5	69	theme	different	788:796	arg1	solutions					798:806	different solutions	788:806	different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC	788:956	The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC.					
36515220	3	70	theme	population	485:494	arg1	diversity					496:504	population diversity	485:504	population diversity	485:504	Somatic cell banks are a strategy for conserving population diversity.					
36515220	7	71	theme	fusiform	1120:1127	arg1	morphology					1129:1138	similar fusiform morphology	1112:1138	similar fusiform morphology	1112:1138	Cells maintained similar fusiform morphology before and after cryopreservation.					
36515220	9	72	theme	apoptosis	1376:1384	arg1	levels					1386:1391	apoptosis levels	1376:1391	apoptosis levels	1376:1391	Similarly, proliferative activity, metabolic activity, and apoptosis levels were not altered by the composition of the cryoprotectants.					
35973533	6	0	theme	plastic	1038:1044	arg1	polymers					1046:1053	the predominant plastic polymers	1022:1053	the predominant plastic polymers	1022:1053	Samples from the Alboran Sea study sites showed that the predominant fiber color was black and the predominant plastic polymers were polyethylene and cellulose.					
35973533	6	0	theme	plastic	1038:1044	arg1	polyethylene					1060:1071	polyethylene	1060:1071	polyethylene	1060:1071	Samples from the Alboran Sea study sites showed that the predominant fiber color was black and the predominant plastic polymers were polyethylene and cellulose.					
35973533	1	1	attach	presence	108:115	arg2	plastic					120:126	plastic	120:126	plastic	120:126	The presence of plastic in the environment has become a major problem for marine ecosystems.					
35973533	1	1	attach	presence	108:115	arg1	environment					135:145	the environment	131:145	the environment	131:145	The presence of plastic in the environment has become a major problem for marine ecosystems.					
35973533	0	2	from	fishes	57:62	arg1	Sea					79:81	the Alboran Sea	67:81	the Alboran Sea (Mediterranean Sea)	67:101	Presence and implications of plastics in wild commercial fishes in the Alboran Sea (Mediterranean Sea).					
35973533	0	2	from	fishes	57:62	arg1	Sea					98:100	Mediterranean Sea	84:100	Mediterranean Sea	84:100	Presence and implications of plastics in wild commercial fishes in the Alboran Sea (Mediterranean Sea).					
35973533	2	3	theme	uptake	252:257	arg1	identification					201:214	The identification	197:214	The identification of the global micro and mesoplastic uptake by commercial fish populations	197:288	The identification of the global micro and mesoplastic uptake by commercial fish populations may allow for a better understanding of their impact.					
35973533	3	4	theme	demersal	487:494	arg1	species					496:502	two demersal species	483:502	two demersal species (Scyliorinus canicula and Mullus barbatus)	483:545	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	4	theme	demersal	487:494	arg1	canicula					517:524	Scyliorinus canicula	505:524	Scyliorinus canicula	505:524	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	4	theme	demersal	487:494	arg1	barbatus					537:544	Mullus barbatus	530:544	Mullus barbatus	530:544	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	5	theme	fish	705:708	arg1	species					710:716	the selected fish species	692:716	the selected fish species	692:716	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	6	theme	Mullus	530:535	arg1	species					496:502	two demersal species	483:502	two demersal species (Scyliorinus canicula and Mullus barbatus)	483:545	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	6	theme	Mullus	530:535	arg1	barbatus					537:544	Mullus barbatus	530:544	Mullus barbatus	530:544	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	7	7	from	occurrence	1223:1232	arg1	tracts					1259:1264	digestive tracts	1249:1264	digestive tracts	1249:1264	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	2	8	theme	commercial	262:271	arg1	populations					278:288	commercial fish populations	262:288	commercial fish populations	262:288	The identification of the global micro and mesoplastic uptake by commercial fish populations may allow for a better understanding of their impact.					
35973533	3	9	dep	Sea	564:566	arg1	Mediterranean					577:589	Mediterranean	577:589	Mediterranean	577:589	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	1	10	theme	marine	178:183	arg1	ecosystems					185:194	marine ecosystems	178:194	marine ecosystems	178:194	The presence of plastic in the environment has become a major problem for marine ecosystems.					
35973533	8	11	theme	commercial	1398:1407	arg1	species					1414:1420	commercial fish species	1398:1420	commercial fish species	1398:1420	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	3	12	from	environment	656:666	arg1	species					710:716	the selected fish species	692:716	the selected fish species	692:716	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	2	13	theme	global	223:228	arg1	micro					230:234	the global micro and mesoplastic uptake	219:257	micro	230:234	The identification of the global micro and mesoplastic uptake by commercial fish populations may allow for a better understanding of their impact.					
35973533	3	14	dep	species	496:502	arg1	species					496:502	two demersal species	483:502	two demersal species (Scyliorinus canicula and Mullus barbatus)	483:545	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	14	dep	species	496:502	arg1	canicula					517:524	Scyliorinus canicula	505:524	Scyliorinus canicula	505:524	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	14	dep	species	496:502	arg1	barbatus					537:544	Mullus barbatus	530:544	Mullus barbatus	530:544	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	1	15	theme	plastic	120:126	arg1	presence					108:115	The presence	104:115	The presence of plastic in the environment	104:145	The presence of plastic in the environment has become a major problem for marine ecosystems.					
35973533	6	16	theme	study	956:960	arg1	sites					962:966	the Alboran Sea study sites	940:966	the Alboran Sea study sites	940:966	Samples from the Alboran Sea study sites showed that the predominant fiber color was black and the predominant plastic polymers were polyethylene and cellulose.					
35973533	0	17	from	implications	13:24	arg1	fishes					57:62	wild commercial fishes	41:62	wild commercial fishes in the Alboran Sea (Mediterranean Sea)	41:101	Presence and implications of plastics in wild commercial fishes in the Alboran Sea (Mediterranean Sea).					
35973533	6	18	from	sites	962:966	arg1	Samples					927:933	Samples	927:933	Samples from the Alboran Sea study sites	927:966	Samples from the Alboran Sea study sites showed that the predominant fiber color was black and the predominant plastic polymers were polyethylene and cellulose.					
35973533	6	19	theme	Alboran	944:950	arg1	sites					962:966	the Alboran Sea study sites	940:966	the Alboran Sea study sites	940:966	Samples from the Alboran Sea study sites showed that the predominant fiber color was black and the predominant plastic polymers were polyethylene and cellulose.					
35973533	0	20	from	Presence	0:7	arg1	fishes					57:62	wild commercial fishes	41:62	wild commercial fishes in the Alboran Sea (Mediterranean Sea)	41:101	Presence and implications of plastics in wild commercial fishes in the Alboran Sea (Mediterranean Sea).					
35973533	7	21	theme	digestive	1249:1257	arg1	tracts					1259:1264	digestive tracts	1249:1264	digestive tracts	1249:1264	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	3	22	from	Sea	564:566	arg1	composition					390:400	composition	390:400	composition	390:400	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	22	from	Sea	564:566	arg1	canicula					517:524	Scyliorinus canicula	505:524	Scyliorinus canicula	505:524	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	22	from	Sea	564:566	arg1	presence					377:384	the presence	373:384	the presence	373:384	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	22	from	Sea	564:566	arg1	species					496:502	two demersal species	483:502	two demersal species (Scyliorinus canicula and Mullus barbatus)	483:545	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	22	from	Sea	564:566	arg1	barbatus					537:544	Mullus barbatus	530:544	Mullus barbatus	530:544	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	22	from	Sea	564:566	arg1	fish					428:431	two pelagic fish	416:431	two pelagic fish (Engraulis encrasicolus and Scomber scombrus)	416:477	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	0	23	theme	Mediterranean	84:96	arg1	Sea					79:81	the Alboran Sea	67:81	the Alboran Sea (Mediterranean Sea)	67:101	Presence and implications of plastics in wild commercial fishes in the Alboran Sea (Mediterranean Sea).					
35973533	0	23	theme	Mediterranean	84:96	arg1	Sea					98:100	Mediterranean Sea	84:100	Mediterranean Sea	84:100	Presence and implications of plastics in wild commercial fishes in the Alboran Sea (Mediterranean Sea).					
35973533	7	24	theme	highest	1122:1128	arg1	occurrence					1138:1147	the highest plastic occurrence	1118:1147	the highest plastic occurrence	1118:1147	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	8	25	theme	marine	1322:1327	arg1	pollution					1337:1345	marine plastic pollution	1322:1345	marine plastic pollution	1322:1345	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	3	26	theme	pelagic	420:426	arg1	fish					428:431	two pelagic fish	416:431	two pelagic fish (Engraulis encrasicolus and Scomber scombrus)	416:477	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	5	27	theme	broader	899:905	arg1	range					920:924	a broader geographical range	897:924	a broader geographical range	897:924	These localized samples were also compared to published values which covered a broader geographical range.					
35973533	6	28	theme	fiber	996:1000	arg1	black					1012:1016	black	1012:1016	black	1012:1016	Samples from the Alboran Sea study sites showed that the predominant fiber color was black and the predominant plastic polymers were polyethylene and cellulose.					
35973533	6	28	theme	fiber	996:1000	arg1	color					1002:1006	the predominant fiber color	980:1006	the predominant fiber color	980:1006	Samples from the Alboran Sea study sites showed that the predominant fiber color was black and the predominant plastic polymers were polyethylene and cellulose.					
35973533	8	29	theme	pollution	1337:1345	arg1	prevalence					1308:1317	The general prevalence	1296:1317	The general prevalence of marine plastic pollution	1296:1345	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	8	29	theme	pollution	1337:1345	arg1	levels					1351:1356	levels	1351:1356	levels of macro- and micro-plastic ingested by commercial fish species in this study	1351:1434	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	0	30	theme	plastics	29:36	arg1	implications					13:24	implications	13:24	implications	13:24	Presence and implications of plastics in wild commercial fishes in the Alboran Sea (Mediterranean Sea).					
35973533	0	30	theme	plastics	29:36	arg1	Presence					0:7	Presence	0:7	Presence	0:7	Presence and implications of plastics in wild commercial fishes in the Alboran Sea (Mediterranean Sea).					
35973533	7	31	theme	Sea	1103:1105	arg1	site					1113:1116	the Alboran Sea study site	1091:1116	the Alboran Sea study site	1091:1116	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	7	32	theme	plastics	1237:1244	arg1	occurrence					1223:1232	the highest occurrence	1211:1232	the highest occurrence of plastics in digestive tracts	1211:1264	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	4	33	theme	Alboran	772:778	arg1	Sea					780:782	the Alboran Sea	768:782	the Alboran Sea	768:782	Samples of these four fish species from sites in the Alboran Sea were studied for ingested plastics.					
35973533	2	34	theme	better	306:311	arg1	understanding					313:325	a better understanding	304:325	a better understanding of their impact	304:341	The identification of the global micro and mesoplastic uptake by commercial fish populations may allow for a better understanding of their impact.					
35973533	0	35	theme	wild	41:44	arg1	fishes					57:62	wild commercial fishes	41:62	wild commercial fishes in the Alboran Sea (Mediterranean Sea)	41:101	Presence and implications of plastics in wild commercial fishes in the Alboran Sea (Mediterranean Sea).					
35973533	8	36	theme	potential	1537:1545	arg1	impacts					1547:1553	potential impacts	1537:1553	potential impacts on marine ecosystems	1537:1574	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	3	37	theme	plastic	629:635	arg1	prevalence					637:646	plastic prevalence	629:646	plastic prevalence	629:646	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	8	38	theme	macro-	1361:1366	arg1	prevalence					1308:1317	The general prevalence	1296:1317	The general prevalence of marine plastic pollution	1296:1345	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	8	38	theme	macro-	1361:1366	arg1	levels					1351:1356	levels	1351:1356	levels of macro- and micro-plastic ingested by commercial fish species in this study	1351:1434	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	8	39	theme	micro-plastic	1372:1384	arg1	prevalence					1308:1317	The general prevalence	1296:1317	The general prevalence of marine plastic pollution	1296:1345	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	8	39	theme	micro-plastic	1372:1384	arg1	levels					1351:1356	levels	1351:1356	levels of macro- and micro-plastic ingested by commercial fish species in this study	1351:1434	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	3	40	from	composition	390:400	arg1	species					496:502	two demersal species	483:502	two demersal species (Scyliorinus canicula and Mullus barbatus)	483:545	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	40	from	composition	390:400	arg1	Sea					564:566	the Alboran Sea	552:566	the Alboran Sea (western Mediterranean)	552:590	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	40	from	composition	390:400	arg1	barbatus					537:544	Mullus barbatus	530:544	Mullus barbatus	530:544	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	40	from	composition	390:400	arg1	canicula					517:524	Scyliorinus canicula	505:524	Scyliorinus canicula	505:524	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	40	from	composition	390:400	arg1	fish					428:431	two pelagic fish	416:431	two pelagic fish (Engraulis encrasicolus and Scomber scombrus)	416:477	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	2	41	theme	impact	336:341	arg1	understanding					313:325	a better understanding	304:325	a better understanding of their impact	304:341	The identification of the global micro and mesoplastic uptake by commercial fish populations may allow for a better understanding of their impact.					
35973533	8	42	dep	plastic	1470:1476	arg1	presence					1478:1485	presence	1478:1485	presence	1478:1485	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	3	43	dep	fish	428:431	arg1	encrasicolus					444:455	Engraulis encrasicolus	434:455	Engraulis encrasicolus	434:455	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	43	dep	fish	428:431	arg1	scombrus					469:476	Scomber scombrus	461:476	Scomber scombrus	461:476	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	7	44	theme	published	1190:1198	arg1	literature					1200:1209	the published literature	1186:1209	the published literature	1186:1209	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	3	45	theme	Scomber	461:467	arg1	scombrus					469:476	Scomber scombrus	461:476	Scomber scombrus	461:476	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	4	46	theme	ingested	801:808	arg1	plastics					810:817	ingested plastics	801:817	ingested plastics	801:817	Samples of these four fish species from sites in the Alboran Sea were studied for ingested plastics.					
35973533	6	47	theme	predominant	1026:1036	arg1	polymers					1046:1053	the predominant plastic polymers	1022:1053	the predominant plastic polymers	1022:1053	Samples from the Alboran Sea study sites showed that the predominant fiber color was black and the predominant plastic polymers were polyethylene and cellulose.					
35973533	6	47	theme	predominant	1026:1036	arg1	polyethylene					1060:1071	polyethylene	1060:1071	polyethylene	1060:1071	Samples from the Alboran Sea study sites showed that the predominant fiber color was black and the predominant plastic polymers were polyethylene and cellulose.					
35973533	8	48	from	impacts	1547:1553	arg1	ecosystems					1565:1574	marine ecosystems	1558:1574	marine ecosystems	1558:1574	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	2	49	theme	mesoplastic	240:250	arg1	uptake					252:257	the global micro and mesoplastic uptake	219:257	uptake	252:257	The identification of the global micro and mesoplastic uptake by commercial fish populations may allow for a better understanding of their impact.					
35973533	3	50	theme	feeding	672:678	arg1	behavior					680:687	feeding behavior	672:687	feeding behavior	672:687	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	51	from	behavior	680:687	arg1	species					710:716	the selected fish species	692:716	the selected fish species	692:716	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	2	52	theme	fish	273:276	arg1	populations					278:288	commercial fish populations	262:288	commercial fish populations	262:288	The identification of the global micro and mesoplastic uptake by commercial fish populations may allow for a better understanding of their impact.					
35973533	1	53	theme	major	160:164	arg1	problem					166:172	a major problem	158:172	a major problem for marine ecosystems	158:194	The presence of plastic in the environment has become a major problem for marine ecosystems.					
35973533	3	54	attach	presence	377:384	arg1	species					496:502	two demersal species	483:502	two demersal species (Scyliorinus canicula and Mullus barbatus)	483:545	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	54	attach	presence	377:384	arg1	canicula					517:524	Scyliorinus canicula	505:524	Scyliorinus canicula	505:524	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	54	attach	presence	377:384	arg1	barbatus					537:544	Mullus barbatus	530:544	Mullus barbatus	530:544	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	54	attach	presence	377:384	arg2	plastic					405:411	plastic	405:411	plastic	405:411	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	54	attach	presence	377:384	arg1	fish					428:431	two pelagic fish	416:431	two pelagic fish (Engraulis encrasicolus and Scomber scombrus)	416:477	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	55	from	prevalence	637:646	arg1	species					710:716	the selected fish species	692:716	the selected fish species	692:716	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	56	theme	selected	696:703	arg1	species					710:716	the selected fish species	692:716	the selected fish species	692:716	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	8	57	theme	fish	1409:1412	arg1	species					1414:1420	commercial fish species	1398:1420	commercial fish species	1398:1420	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	7	58	located	found	1270:1274	arg2	occurrence					1223:1232	the highest occurrence	1211:1232	the highest occurrence of plastics in digestive tracts	1211:1264	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	7	58	located	found	1270:1274	arg1	encrasicolus					1282:1293	E. encrasicolus	1279:1293	E. encrasicolus	1279:1293	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	7	58	located	found	1270:1274	arg1	literature					1200:1209	the published literature	1186:1209	the published literature	1186:1209	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	2	59	theme	micro	230:234	arg1	identification					201:214	The identification	197:214	The identification of the global micro and mesoplastic uptake by commercial fish populations	197:288	The identification of the global micro and mesoplastic uptake by commercial fish populations may allow for a better understanding of their impact.					
35973533	4	60	from	sites	759:763	arg1	Samples					719:725	Samples	719:725	Samples of these four fish species from sites in the Alboran Sea	719:782	Samples of these four fish species from sites in the Alboran Sea were studied for ingested plastics.					
35973533	4	60	from	sites	759:763	arg1	Sea					780:782	the Alboran Sea	768:782	the Alboran Sea	768:782	Samples of these four fish species from sites in the Alboran Sea were studied for ingested plastics.					
35973533	5	61	theme	published	866:874	arg1	values					876:881	published values	866:881	published values which covered a broader geographical range	866:924	These localized samples were also compared to published values which covered a broader geographical range.					
35973533	3	62	theme	Scyliorinus	505:515	arg1	species					496:502	two demersal species	483:502	two demersal species (Scyliorinus canicula and Mullus barbatus)	483:545	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	62	theme	Scyliorinus	505:515	arg1	canicula					517:524	Scyliorinus canicula	505:524	Scyliorinus canicula	505:524	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	4	63	theme	fish	741:744	arg1	species					746:752	these four fish species	730:752	these four fish species	730:752	Samples of these four fish species from sites in the Alboran Sea were studied for ingested plastics.					
35973533	3	64	theme	Alboran	556:562	arg1	Sea					564:566	the Alboran Sea	552:566	the Alboran Sea (western Mediterranean)	552:590	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	5	65	theme	localized	826:834	arg1	samples					836:842	These localized samples	820:842	These localized samples	820:842	These localized samples were also compared to published values which covered a broader geographical range.					
35973533	0	66	theme	Alboran	71:77	arg1	Sea					79:81	the Alboran Sea	67:81	the Alboran Sea (Mediterranean Sea)	67:101	Presence and implications of plastics in wild commercial fishes in the Alboran Sea (Mediterranean Sea).					
35973533	0	66	theme	Alboran	71:77	arg1	Sea					98:100	Mediterranean Sea	84:100	Mediterranean Sea	84:100	Presence and implications of plastics in wild commercial fishes in the Alboran Sea (Mediterranean Sea).					
35973533	6	67	theme	Sea	952:954	arg1	sites					962:966	the Alboran Sea study sites	940:966	the Alboran Sea study sites	940:966	Samples from the Alboran Sea study sites showed that the predominant fiber color was black and the predominant plastic polymers were polyethylene and cellulose.					
35973533	3	68	from	presence	377:384	arg1	species					496:502	two demersal species	483:502	two demersal species (Scyliorinus canicula and Mullus barbatus)	483:545	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	68	from	presence	377:384	arg1	Sea					564:566	the Alboran Sea	552:566	the Alboran Sea (western Mediterranean)	552:590	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	68	from	presence	377:384	arg1	barbatus					537:544	Mullus barbatus	530:544	Mullus barbatus	530:544	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	68	from	presence	377:384	arg1	canicula					517:524	Scyliorinus canicula	505:524	Scyliorinus canicula	505:524	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	68	from	presence	377:384	arg1	fish					428:431	two pelagic fish	416:431	two pelagic fish (Engraulis encrasicolus and Scomber scombrus)	416:477	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	5	69	theme	geographical	907:918	arg1	range					920:924	a broader geographical range	897:924	a broader geographical range	897:924	These localized samples were also compared to published values which covered a broader geographical range.					
35973533	7	70	theme	plastic	1130:1136	arg1	occurrence					1138:1147	the highest plastic occurrence	1118:1147	the highest plastic occurrence	1118:1147	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	3	71	theme	plastic	405:411	arg1	composition					390:400	composition	390:400	composition	390:400	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	3	71	theme	plastic	405:411	arg1	presence					377:384	the presence	373:384	the presence	373:384	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	1	72	from	presence	108:115	arg1	environment					135:145	the environment	131:145	the environment	131:145	The presence of plastic in the environment has become a major problem for marine ecosystems.					
35973533	8	73	theme	general	1300:1306	arg1	prevalence					1308:1317	The general prevalence	1296:1317	The general prevalence of marine plastic pollution	1296:1345	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	7	74	theme	study	1107:1111	arg1	site					1113:1116	the Alboran Sea study site	1091:1116	the Alboran Sea study site	1091:1116	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	7	75	theme	Alboran	1095:1101	arg1	site					1113:1116	the Alboran Sea study site	1091:1116	the Alboran Sea study site	1091:1116	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	3	76	dep	environment	656:666	arg1	the					652:654	the	652:654	the	652:654	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	6	77	theme	predominant	984:994	arg1	black					1012:1016	black	1012:1016	black	1012:1016	Samples from the Alboran Sea study sites showed that the predominant fiber color was black and the predominant plastic polymers were polyethylene and cellulose.					
35973533	6	77	theme	predominant	984:994	arg1	color					1002:1006	the predominant fiber color	980:1006	the predominant fiber color	980:1006	Samples from the Alboran Sea study sites showed that the predominant fiber color was black and the predominant plastic polymers were polyethylene and cellulose.					
35973533	8	78	theme	plastic	1329:1335	arg1	pollution					1337:1345	marine plastic pollution	1322:1345	marine plastic pollution	1322:1345	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	7	79	located	found	1153:1157	arg1	site					1113:1116	the Alboran Sea study site	1091:1116	the Alboran Sea study site	1091:1116	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	7	79	located	found	1153:1157	arg1	scombrus					1165:1172	S. scombrus	1162:1172	S. scombrus	1162:1172	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	7	79	located	found	1153:1157	arg2	occurrence					1138:1147	the highest plastic occurrence	1118:1147	the highest plastic occurrence	1118:1147	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	0	80	theme	commercial	46:55	arg1	fishes					57:62	wild commercial fishes	41:62	wild commercial fishes in the Alboran Sea (Mediterranean Sea)	41:101	Presence and implications of plastics in wild commercial fishes in the Alboran Sea (Mediterranean Sea).					
35973533	7	81	theme	highest	1215:1221	arg1	occurrence					1223:1232	the highest occurrence	1211:1232	the highest occurrence of plastics in digestive tracts	1211:1264	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	8	82	theme	marine	1558:1563	arg1	ecosystems					1565:1574	marine ecosystems	1558:1574	marine ecosystems	1558:1574	The general prevalence of marine plastic pollution and levels of macro- and micro-plastic ingested by commercial fish species in this study support the idea that quantifying plastic presence and composition may be essential to understanding potential impacts on marine ecosystems.					
35973533	7	83	dep	found	1153:1157	arg1	whereas					1175:1181	whereas	1175:1181	whereas	1175:1181	At the Alboran Sea study site the highest plastic occurrence was found in S. scombrus, whereas in the published literature the highest occurrence of plastics in digestive tracts was found in E. encrasicolus.					
35973533	3	84	theme	Engraulis	434:442	arg1	encrasicolus					444:455	Engraulis encrasicolus	434:455	Engraulis encrasicolus	434:455	This study aims to determine the presence and composition of plastic in two pelagic fish (Engraulis encrasicolus and Scomber scombrus) and two demersal species (Scyliorinus canicula and Mullus barbatus) from the Alboran Sea (western Mediterranean) to quantify the relationship between plastic prevalence and the environment and feeding behavior in the selected fish species.					
35973533	4	85	theme	species	746:752	arg1	Samples					719:725	Samples	719:725	Samples of these four fish species from sites in the Alboran Sea	719:782	Samples of these four fish species from sites in the Alboran Sea were studied for ingested plastics.					
36278723	1	0	theme	capable	141:147	arg1	polyp					134:138	a freshwater solitary polyp	112:138	a freshwater solitary polyp	112:138	Hydra is a freshwater solitary polyp, capable of temporary adhesion to underwater surfaces.					
36278723	1	0	theme	capable	141:147	arg1	Hydra					103:107	Hydra	103:107	Hydra	103:107	Hydra is a freshwater solitary polyp, capable of temporary adhesion to underwater surfaces.					
36278723	5	1	theme	N-acetylgalactosamine	713:733	arg1	presence					680:687	the presence	676:687	the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material	676:779	The results indicated the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material.					
36278723	4	2	theme	Hydra	552:556	arg1	composition					533:543	the glycan composition	522:543	the glycan composition of the Hydra adhesive	522:565	This project aimed to characterize the glycan composition of the Hydra adhesive, using a set of 23 commercially available lectins to label Hydra cells and footprints.					
36278723	0	3	from	Involvement	4:14	arg1	Adhesion					65:72	Hydra Temporary Adhesion	49:72	Hydra Temporary Adhesion	49:72	The Involvement of Cell-Type-Specific Glycans in Hydra Temporary Adhesion Revealed by a Lectin Screen.					
36278723	5	4	from	presence	680:687	arg1	material					772:779	the adhesive material	759:779	the adhesive material	759:779	The results indicated the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material.					
36278723	8	5	theme	radial	1121:1126	arg1	fibers					1135:1140	radial muscle fibers	1121:1140	radial muscle fibers	1121:1140	Additionally, some unexpected patterns were uncovered, such as structures associated with radial muscle fibers and endodermal gland cells in the hypostome of developing buds.					
36278723	0	6	theme	Lectin	88:93	arg1	Screen					95:100	a Lectin Screen	86:100	a Lectin Screen	86:100	The Involvement of Cell-Type-Specific Glycans in Hydra Temporary Adhesion Revealed by a Lectin Screen.					
36278723	7	7	dep	cells	962:966	arg1	e.g.					984:987	Hydra cells and structures, e.g., many labeled as glycan-rich nematocytes	956:1028	e.g.	984:987	Furthermore, lectins might serve as a marker for Hydra cells and structures, e.g., many labeled as glycan-rich nematocytes.					
36278723	1	8	theme	solitary	125:132	arg1	polyp					134:138	a freshwater solitary polyp	112:138	a freshwater solitary polyp	112:138	Hydra is a freshwater solitary polyp, capable of temporary adhesion to underwater surfaces.					
36278723	1	8	theme	solitary	125:132	arg1	Hydra					103:107	Hydra	103:107	Hydra	103:107	Hydra is a freshwater solitary polyp, capable of temporary adhesion to underwater surfaces.					
36278723	1	9	theme	temporary	152:160	arg1	adhesion					162:169	temporary adhesion	152:169	temporary adhesion to underwater surfaces	152:192	Hydra is a freshwater solitary polyp, capable of temporary adhesion to underwater surfaces.					
36278723	3	10	theme	standard	375:382	arg1	system					390:395	a standard model system	373:395	a standard model system	373:395	Despite Hydra constituting a standard model system in stem cell biology and tissue regeneration, few studies have addressed its bioadhesion.					
36278723	5	11	theme	mannose	748:754	arg1	presence					680:687	the presence	676:687	the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material	676:779	The results indicated the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material.					
36278723	5	12	attach	presence	680:687	arg2	mannose					748:754	mannose	748:754	mannose	748:754	The results indicated the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material.					
36278723	5	12	attach	presence	680:687	arg2	fucose					736:741	fucose	736:741	fucose	736:741	The results indicated the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material.					
36278723	5	12	attach	presence	680:687	arg2	N-acetylgalactosamine					713:733	N-acetylgalactosamine	713:733	N-acetylgalactosamine	713:733	The results indicated the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material.					
36278723	5	12	attach	presence	680:687	arg1	material					772:779	the adhesive material	759:779	the adhesive material	759:779	The results indicated the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material.					
36278723	5	12	attach	presence	680:687	arg2	N-acetylglucosamine					692:710	N-acetylglucosamine	692:710	N-acetylglucosamine	692:710	The results indicated the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material.					
36278723	1	13	theme	adhesion	162:169	arg1	capable					141:147	capable	141:147	capable	141:147	Hydra is a freshwater solitary polyp, capable of temporary adhesion to underwater surfaces.					
36278723	8	14	theme	endodermal	1146:1155	arg1	cells					1163:1167	endodermal gland cells	1146:1167	endodermal gland cells	1146:1167	Additionally, some unexpected patterns were uncovered, such as structures associated with radial muscle fibers and endodermal gland cells in the hypostome of developing buds.					
36278723	4	15	theme	glycan	526:531	arg1	composition					533:543	the glycan composition	522:543	the glycan composition of the Hydra adhesive	522:565	This project aimed to characterize the glycan composition of the Hydra adhesive, using a set of 23 commercially available lectins to label Hydra cells and footprints.					
36278723	8	16	theme	buds	1200:1203	arg1	hypostome					1176:1184	the hypostome	1172:1184	the hypostome of developing buds	1172:1203	Additionally, some unexpected patterns were uncovered, such as structures associated with radial muscle fibers and endodermal gland cells in the hypostome of developing buds.					
36278723	8	17	from	fibers	1135:1140	arg1	hypostome					1176:1184	the hypostome	1172:1184	the hypostome of developing buds	1172:1203	Additionally, some unexpected patterns were uncovered, such as structures associated with radial muscle fibers and endodermal gland cells in the hypostome of developing buds.					
36278723	3	18	theme	stem	400:403	arg1	biology					410:416	stem cell biology	400:416	stem cell biology	400:416	Despite Hydra constituting a standard model system in stem cell biology and tissue regeneration, few studies have addressed its bioadhesion.					
36278723	0	19	theme	Cell-Type-Specific	19:36	arg1	Glycans					38:44	Cell-Type-Specific Glycans	19:44	Cell-Type-Specific Glycans	19:44	The Involvement of Cell-Type-Specific Glycans in Hydra Temporary Adhesion Revealed by a Lectin Screen.					
36278723	8	20	theme	muscle	1128:1133	arg1	fibers					1135:1140	radial muscle fibers	1121:1140	radial muscle fibers	1121:1140	Additionally, some unexpected patterns were uncovered, such as structures associated with radial muscle fibers and endodermal gland cells in the hypostome of developing buds.					
36278723	2	21	theme	disc	286:289	arg1	cells					291:295	its basal disc cells	276:295	its basal disc cells	276:295	The reversible attachment is based on an adhesive material that is secreted from its basal disc cells and left behind on the substrate as a footprint.					
36278723	8	22	theme	gland	1157:1161	arg1	cells					1163:1167	endodermal gland cells	1146:1167	endodermal gland cells	1146:1167	Additionally, some unexpected patterns were uncovered, such as structures associated with radial muscle fibers and endodermal gland cells in the hypostome of developing buds.					
36278723	2	23	theme	basal	280:284	arg1	cells					291:295	its basal disc cells	276:295	its basal disc cells	276:295	The reversible attachment is based on an adhesive material that is secreted from its basal disc cells and left behind on the substrate as a footprint.					
36278723	3	24	theme	model	384:388	arg1	system					390:395	a standard model system	373:395	a standard model system	373:395	Despite Hydra constituting a standard model system in stem cell biology and tissue regeneration, few studies have addressed its bioadhesion.					
36278723	8	25	theme	unexpected	1050:1059	arg1	patterns					1061:1068	some unexpected patterns	1045:1068	some unexpected patterns	1045:1068	Additionally, some unexpected patterns were uncovered, such as structures associated with radial muscle fibers and endodermal gland cells in the hypostome of developing buds.					
36278723	1	26	theme	underwater	174:183	arg1	surfaces					185:192	underwater surfaces	174:192	underwater surfaces	174:192	Hydra is a freshwater solitary polyp, capable of temporary adhesion to underwater surfaces.					
36278723	4	27	theme	lectins	609:615	arg1	lectins					609:615	23 commercially available lectins	583:615	23 commercially available lectins	583:615	This project aimed to characterize the glycan composition of the Hydra adhesive, using a set of 23 commercially available lectins to label Hydra cells and footprints.					
36278723	4	27	theme	lectins	609:615	arg1	set					576:578	a set	574:578	a set of 23 commercially available lectins	574:615	This project aimed to characterize the glycan composition of the Hydra adhesive, using a set of 23 commercially available lectins to label Hydra cells and footprints.					
36278723	3	28	theme	cell	405:408	arg1	biology					410:416	stem cell biology	400:416	stem cell biology	400:416	Despite Hydra constituting a standard model system in stem cell biology and tissue regeneration, few studies have addressed its bioadhesion.					
36278723	0	29	theme	Glycans	38:44	arg1	Involvement					4:14	The Involvement	0:14	The Involvement of Cell-Type-Specific Glycans in Hydra Temporary Adhesion	0:72	The Involvement of Cell-Type-Specific Glycans in Hydra Temporary Adhesion Revealed by a Lectin Screen.					
36278723	3	30	theme	tissue	422:427	arg1	regeneration					429:440	tissue regeneration	422:440	tissue regeneration	422:440	Despite Hydra constituting a standard model system in stem cell biology and tissue regeneration, few studies have addressed its bioadhesion.					
36278723	0	31	theme	Temporary	55:63	arg1	Adhesion					65:72	Hydra Temporary Adhesion	49:72	Hydra Temporary Adhesion	49:72	The Involvement of Cell-Type-Specific Glycans in Hydra Temporary Adhesion Revealed by a Lectin Screen.					
36278723	5	32	theme	adhesive	763:770	arg1	material					772:779	the adhesive material	759:779	the adhesive material	759:779	The results indicated the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material.					
36278723	7	33	theme	glycan-rich	1006:1016	arg1	nematocytes					1018:1028	glycan-rich nematocytes	1006:1028	glycan-rich nematocytes	1006:1028	Furthermore, lectins might serve as a marker for Hydra cells and structures, e.g., many labeled as glycan-rich nematocytes.					
36278723	0	34	theme	Hydra	49:53	arg1	Adhesion					65:72	Hydra Temporary Adhesion	49:72	Hydra Temporary Adhesion	49:72	The Involvement of Cell-Type-Specific Glycans in Hydra Temporary Adhesion Revealed by a Lectin Screen.					
36278723	2	35	theme	reversible	199:208	arg1	attachment					210:219	The reversible attachment	195:219	The reversible attachment	195:219	The reversible attachment is based on an adhesive material that is secreted from its basal disc cells and left behind on the substrate as a footprint.					
36278723	4	36	theme	Hydra	626:630	arg1	cells					632:636	Hydra cells	626:636	Hydra cells	626:636	This project aimed to characterize the glycan composition of the Hydra adhesive, using a set of 23 commercially available lectins to label Hydra cells and footprints.					
36278723	6	37	theme	meshwork-like	806:818	arg1	substructure					820:831	a meshwork-like substructure	804:831	a meshwork-like substructure	804:831	The labeling revealed a meshwork-like substructure in the footprints, implying that the adhesive is mainly formed by fibers.					
36278723	1	38	theme	freshwater	114:123	arg1	polyp					134:138	a freshwater solitary polyp	112:138	a freshwater solitary polyp	112:138	Hydra is a freshwater solitary polyp, capable of temporary adhesion to underwater surfaces.					
36278723	1	38	theme	freshwater	114:123	arg1	Hydra					103:107	Hydra	103:107	Hydra	103:107	Hydra is a freshwater solitary polyp, capable of temporary adhesion to underwater surfaces.					
36278723	2	39	theme	left	301:304	arg1	behind					306:311	left behind	301:311	left behind	301:311	The reversible attachment is based on an adhesive material that is secreted from its basal disc cells and left behind on the substrate as a footprint.					
36278723	3	40	theme	few	443:445	arg1	studies					447:453	few studies	443:453	few studies	443:453	Despite Hydra constituting a standard model system in stem cell biology and tissue regeneration, few studies have addressed its bioadhesion.					
36278723	4	41	theme	available	599:607	arg1	lectins					609:615	23 commercially available lectins	583:615	23 commercially available lectins	583:615	This project aimed to characterize the glycan composition of the Hydra adhesive, using a set of 23 commercially available lectins to label Hydra cells and footprints.					
36278723	2	42	theme	adhesive	236:243	arg1	material					245:252	an adhesive material	233:252	an adhesive material that is secreted from its basal disc cells and left behind on the substrate as a footprint	233:343	The reversible attachment is based on an adhesive material that is secreted from its basal disc cells and left behind on the substrate as a footprint.					
36278723	2	42	theme	adhesive	236:243	arg1	footprint					335:343	a footprint	333:343	a footprint	333:343	The reversible attachment is based on an adhesive material that is secreted from its basal disc cells and left behind on the substrate as a footprint.					
36278723	7	43	theme	Hydra	956:960	arg1	many					990:993	many	990:993	many	990:993	Furthermore, lectins might serve as a marker for Hydra cells and structures, e.g., many labeled as glycan-rich nematocytes.					
36278723	7	43	theme	Hydra	956:960	arg1	cells					962:966	Hydra cells	956:966	Hydra cells	956:966	Furthermore, lectins might serve as a marker for Hydra cells and structures, e.g., many labeled as glycan-rich nematocytes.					
36278723	8	44	theme	developing	1189:1198	arg1	buds					1200:1203	developing buds	1189:1203	developing buds	1189:1203	Additionally, some unexpected patterns were uncovered, such as structures associated with radial muscle fibers and endodermal gland cells in the hypostome of developing buds.					
36278723	4	45	theme	adhesive	558:565	arg1	Hydra					552:556	the Hydra adhesive	548:565	the Hydra adhesive	548:565	This project aimed to characterize the glycan composition of the Hydra adhesive, using a set of 23 commercially available lectins to label Hydra cells and footprints.					
36278723	5	46	theme	fucose	736:741	arg1	presence					680:687	the presence	676:687	the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material	676:779	The results indicated the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material.					
36278723	8	47	from	cells	1163:1167	arg1	hypostome					1176:1184	the hypostome	1172:1184	the hypostome of developing buds	1172:1203	Additionally, some unexpected patterns were uncovered, such as structures associated with radial muscle fibers and endodermal gland cells in the hypostome of developing buds.					
36278723	5	48	theme	N-acetylglucosamine	692:710	arg1	presence					680:687	the presence	676:687	the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material	676:779	The results indicated the presence of N-acetylglucosamine, N-acetylgalactosamine, fucose, and mannose in the adhesive material.					
37354614	10	0	theme	P	1847:1847	arg1	<					1849:1849	P < 0.05	1847:1854	P < 0.05	1847:1854	XOS supplementation increased (P < 0.05) ATP2B1 expression.					
37354614	15	1	theme	Eimeria	2368:2374	arg1	challenge					2376:2384	Eimeria challenge	2368:2384	Eimeria challenge	2368:2384	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	0	2	theme	broiler	168:174	arg1	chickens					176:183	Eimeria-challenged broiler chickens	149:183	Eimeria-challenged broiler chickens	149:183	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	18	3	with	consistent	2959:2968	arg1	concentration					2993:3005	decreased cecal N concentration	2975:3005	decreased cecal N concentration	2975:3005	All the additives decreased the cecal content of branched-chain fatty acids, consistent with decreased cecal N concentration, although the effects were more pronounced in unchallenged birds.					
37354614	6	4	theme	Supplemental	1225:1236	arg1	protease					1238:1245	Supplemental protease	1225:1245	Supplemental protease	1225:1245	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	1	5	theme	male	239:242	arg1	chicks					252:257	392 Cobb 500 off-sex male broiler chicks	218:257	392 Cobb 500 off-sex male broiler chicks	218:257	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	6	6	contain	had	1339:1341	arg1	protease					1238:1245	Supplemental protease	1225:1245	Supplemental protease	1225:1245	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	6	6	contain	had	1339:1341	arg2	effect					1346:1351	no effect	1343:1351	no effect	1343:1351	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	8	7	dep	increased	1534:1542	arg1	<					1547:1547	P < 0.01	1545:1552	P < 0.01	1545:1552	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	11	8	theme	P	1896:1896	arg1	<					1898:1898	P < 0.05	1896:1903	P < 0.05	1896:1903	Protease decreased (P < 0.05) isobutyrate concentration in unchallenged treatments but not in challenged treatments.					
37354614	0	9	from	microbiota	135:144	arg1	chickens					176:183	Eimeria-challenged broiler chickens	149:183	Eimeria-challenged broiler chickens	149:183	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	13	10	theme	P	2186:2186	arg1	<					2188:2188	P < 0.05	2186:2193	P < 0.05	2186:2193	The supplementation of PRO + XYL or XOS increased (P < 0.05) cecal butyrate or decreased cecal isobutyrate concentrations, respectively.					
37354614	5	11	theme	nutrient	1203:1210	arg1	utilization					1212:1222	nutrient utilization	1203:1222	nutrient utilization	1203:1222	Eimeria depressed (P < 0.01) growth performance and nutrient utilization.					
37354614	18	12	from	birds	3066:3070	arg1	effects					3021:3027	the effects	3017:3027	the effects	3017:3027	All the additives decreased the cecal content of branched-chain fatty acids, consistent with decreased cecal N concentration, although the effects were more pronounced in unchallenged birds.					
37354614	18	12	from	birds	3066:3070	arg1	pronounced					3039:3048	pronounced	3039:3048	pronounced	3039:3048	All the additives decreased the cecal content of branched-chain fatty acids, consistent with decreased cecal N concentration, although the effects were more pronounced in unchallenged birds.					
37354614	13	13	theme	decreased	2214:2222	arg1	concentrations					2242:2255	decreased cecal isobutyrate concentrations	2214:2255	decreased cecal isobutyrate concentrations	2214:2255	The supplementation of PRO + XYL or XOS increased (P < 0.05) cecal butyrate or decreased cecal isobutyrate concentrations, respectively.					
37354614	1	14	theme	chicks	252:257	arg1	total					209:213	A total	207:213	A total of 392 Cobb 500 off-sex male broiler chicks	207:257	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	10	15	theme	ATP2B1	1857:1862	arg1	expression					1864:1873	ATP2B1 expression	1857:1873	ATP2B1 expression	1857:1873	XOS supplementation increased (P < 0.05) ATP2B1 expression.					
37354614	15	16	theme	microbial	2425:2433	arg1	richness					2435:2442	the microbial richness	2421:2442	the microbial richness (Observed features)	2421:2462	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	15	16	theme	microbial	2425:2433	arg1	features					2454:2461	Observed features	2445:2461	Observed features	2445:2461	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	12	17	theme	saccharolytic	2028:2040	arg1	SCFAs					2042:2046	cecal saccharolytic SCFAs	2022:2046	(P < 0.01) cecal saccharolytic SCFAs acetate and propionate	2011:2069	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	12	17	theme	saccharolytic	2028:2040	arg1	<					2014:2014	P < 0.01	2012:2019	P < 0.01	2012:2019	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	12	17	theme	saccharolytic	2028:2040	arg1	acetate					2048:2054	acetate	2048:2054	acetate	2048:2054	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	12	17	theme	saccharolytic	2028:2040	arg1	propionate					2060:2069	propionate	2060:2069	propionate	2060:2069	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	9	18	theme	D28K	1749:1752	arg1	<					1682:1682	P < 0.05	1680:1687	P < 0.05	1680:1687	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	9	18	theme	D28K	1749:1752	arg1	expressions					1690:1700	expressions	1690:1700	(P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1	1679:1768	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	1	19	theme	ileal	422:426	arg1	digestibility					428:440	ileal digestibility	422:440	ileal digestibility	422:440	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	18	20	theme	cecal	2914:2918	arg1	content					2920:2926	the cecal content	2910:2926	the cecal content of branched-chain fatty acids	2910:2956	All the additives decreased the cecal content of branched-chain fatty acids, consistent with decreased cecal N concentration, although the effects were more pronounced in unchallenged birds.					
37354614	6	21	theme	feed	1288:1291	arg1	intake					1293:1298	feed intake	1288:1298	feed intake	1288:1298	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	9	22	dep	increased	1774:1782	arg1	<					1787:1787	P < 0.01	1785:1792	P < 0.01	1785:1792	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	2	23	theme	factorial	677:685	arg1	arrangement					687:697	a 4 × 2 factorial arrangement	669:697	a 4 × 2 factorial arrangement	669:697	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	9	24	theme	NPT2	1755:1758	arg1	<					1682:1682	P < 0.05	1680:1687	P < 0.05	1680:1687	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	9	24	theme	NPT2	1755:1758	arg1	expressions					1690:1700	expressions	1690:1700	(P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1	1679:1768	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	8	25	theme	tract	1561:1565	arg1	retention					1567:1575	total tract retention	1555:1575	total tract retention of Ca	1555:1581	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	2	26	theme	meal	716:719	arg1	diet					721:724	1) corn-soybean meal diet	700:724	1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge	700:951	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	12	27	dep	increased	2075:2083	arg1	<					2088:2088	P < 0.01	2086:2093	P < 0.01	2086:2093	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	0	28	theme	short-chain	106:116	arg1	acids					124:128	short-chain fatty acids	106:128	short-chain fatty acids	106:128	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	1	29	theme	xylo-oligosaccharides	339:359	arg1	effect					305:310	the effect	301:310	the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp	301:612	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	6	30	theme	body	1267:1270	arg1	gain					1279:1282	body weight gain	1267:1282	body weight gain	1267:1282	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	17	31	theme	PRO	2811:2813	arg1	supplementation					2822:2836	PRO or XOS supplementation	2811:2836	PRO or XOS supplementation	2811:2836	In conclusion, PRO or XOS supplementation individually improved nutrient utilization.					
37354614	10	32	dep	increased	1836:1844	arg1	<					1849:1849	P < 0.05	1847:1854	P < 0.05	1847:1854	XOS supplementation increased (P < 0.05) ATP2B1 expression.					
37354614	14	33	theme	challenged	2351:2360	arg1	ones					2362:2365	challenged ones	2351:2365	unchallenged birds but not challenged ones	2324:2365	PRO + XYL and XOS decreased cecal protein levels in unchallenged birds but not challenged ones.					
37354614	7	34	theme	nutrient	1464:1471	arg1	utilization					1473:1483	nutrient utilization	1464:1483	nutrient utilization	1464:1483	There was no interaction between infection and feed supplementation for nutrient utilization.					
37354614	16	35	theme	significant	2741:2751	arg1	effect					2753:2758	any significant effect	2737:2758	any significant effect	2737:2758	In contrast, none of the additives had any significant effect on the cecal microbial composition.					
37354614	9	36	theme	P	1785:1785	arg1	<					1787:1787	P < 0.01	1785:1792	P < 0.01	1785:1792	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	9	37	theme	CaSR	1734:1737	arg1	<					1682:1682	P < 0.05	1680:1687	P < 0.05	1680:1687	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	9	37	theme	CaSR	1734:1737	arg1	expressions					1690:1700	expressions	1690:1700	(P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1	1679:1768	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	8	38	theme	Ca	1580:1581	arg1	retention					1567:1575	total tract retention	1555:1575	total tract retention of Ca	1555:1581	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	1	39	dep	utilization	409:419	arg1	digestibility					428:440	ileal digestibility	422:440	ileal digestibility	422:440	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	39	dep	utilization	409:419	arg1	retention					458:466	total tract retention	446:466	total tract retention	446:466	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	16	40	theme	additives	2723:2731	arg1	none					2711:2714	none	2711:2714	none of the additives	2711:2731	In contrast, none of the additives had any significant effect on the cecal microbial composition.					
37354614	0	41	from	utilization	93:103	arg1	chickens					176:183	Eimeria-challenged broiler chickens	149:183	Eimeria-challenged broiler chickens	149:183	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	14	42	theme	unchallenged	2324:2335	arg1	birds					2337:2341	unchallenged birds	2324:2341	unchallenged birds but not challenged ones	2324:2365	PRO + XYL and XOS decreased cecal protein levels in unchallenged birds but not challenged ones.					
37354614	11	43	theme	challenged	1970:1979	arg1	treatments					1981:1990	challenged treatments	1970:1990	challenged treatments	1970:1990	Protease decreased (P < 0.05) isobutyrate concentration in unchallenged treatments but not in challenged treatments.					
37354614	8	44	theme	tract	1621:1625	arg1	retention					1627:1635	total tract retention	1615:1635	total tract retention of N, P, AME, and AMEn	1615:1658	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	1	45	theme	growth	380:385	arg1	utilization					409:419	nutrient utilization	400:419	nutrient utilization (ileal digestibility and total tract retention)	400:467	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	45	theme	growth	380:385	arg1	expression					475:484	gene expression	470:484	gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp	470:612	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	45	theme	growth	380:385	arg1	performance					387:397	growth performance	380:397	growth performance	380:397	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	8	46	theme	PRO	1517:1519	arg1	supplementations					1490:1505	The supplementations	1486:1505	The supplementations of either PRO or XOS	1486:1526	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	12	47	dep	SCFAs	2042:2046	arg1	SCFAs					2042:2046	cecal saccharolytic SCFAs	2022:2046	(P < 0.01) cecal saccharolytic SCFAs acetate and propionate	2011:2069	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	12	47	dep	SCFAs	2042:2046	arg1	propionate					2060:2069	propionate	2060:2069	propionate	2060:2069	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	12	47	dep	SCFAs	2042:2046	arg1	acetate					2048:2054	acetate	2048:2054	acetate	2048:2054	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	18	48	theme	decreased	2975:2983	arg1	concentration					2993:3005	decreased cecal N concentration	2975:3005	decreased cecal N concentration	2975:3005	All the additives decreased the cecal content of branched-chain fatty acids, consistent with decreased cecal N concentration, although the effects were more pronounced in unchallenged birds.					
37354614	1	49	theme	microbiota	555:564	arg1	profile					566:572	microbiota profile	555:572	microbiota profile	555:572	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	0	50	theme	xylo-oligosaccharides	39:59	arg1	effects					4:10	The effects	0:10	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens	0:183	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	6	51	theme	prechallenge	1307:1318	arg1	phase					1320:1324	the prechallenge phase	1303:1324	the prechallenge phase (d 0-15)	1303:1333	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	6	51	theme	prechallenge	1307:1318	arg1	0-15					1329:1332	d 0-15	1327:1332	d 0-15	1327:1332	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	4	52	from	oocysts	1134:1140	arg1	d					1145:1145	d 15	1145:1148	d 15	1145:1148	Challenged groups were inoculated with a solution containing E. maxima, E. acervulina, and E. tenella oocysts on d 15.					
37354614	2	53	from	old	631:633	arg1	Chicks					615:620	Chicks	615:620	Chicks at 0-day old	615:633	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	0	54	theme	growth	64:69	arg1	performance					71:81	growth performance	64:81	growth performance	64:81	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	2	55	theme	g/kg	807:810	arg1	protease					812:819	0.2 g/kg protease	803:819	0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL)	803:863	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	16	56	theme	cecal	2767:2771	arg1	composition					2783:2793	the cecal microbial composition	2763:2793	the cecal microbial composition	2763:2793	In contrast, none of the additives had any significant effect on the cecal microbial composition.					
37354614	6	57	theme	infection	1364:1372	arg1	d					1382:1382	d 15-21	1382:1388	d 15-21	1382:1388	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	6	57	theme	infection	1364:1372	arg1	period					1374:1379	the infection period	1360:1379	the infection period (d 15-21)	1360:1389	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	18	58	theme	N	2991:2991	arg1	concentration					2993:3005	decreased cecal N concentration	2975:3005	decreased cecal N concentration	2975:3005	All the additives decreased the cecal content of branched-chain fatty acids, consistent with decreased cecal N concentration, although the effects were more pronounced in unchallenged birds.					
37354614	2	59	theme	xylanase	844:851	arg1	g/kg					839:842	0.1 g/kg xylanase	835:851	0.1 g/kg xylanase (PRO + XYL)	835:863	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	2	59	theme	xylanase	844:851	arg1	PRO + XYL					854:862	PRO + XYL	854:862	PRO + XYL	854:862	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	4	60	theme	Challenged	1032:1041	arg1	groups					1043:1048	Challenged groups	1032:1048	Challenged groups	1032:1048	Challenged groups were inoculated with a solution containing E. maxima, E. acervulina, and E. tenella oocysts on d 15.					
37354614	9	61	theme	P	1680:1680	arg1	<					1682:1682	P < 0.05	1680:1687	P < 0.05	1680:1687	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	9	61	theme	P	1680:1680	arg1	expressions					1690:1700	expressions	1690:1700	(P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1	1679:1768	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	1	62	theme	short-chain	518:528	arg1	acids					536:540	cecal short-chain fatty acids	512:540	cecal short-chain fatty acids (SCFAs)	512:548	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	62	theme	short-chain	518:528	arg1	SCFAs					543:547	SCFAs	543:547	SCFAs	543:547	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	19	63	theme	feed	3098:3101	arg1	additives					3103:3111	the feed additives	3094:3111	the feed additives	3094:3111	In addition, none of the feed additives impacted the Eimeria-induced microbial perturbation.					
37354614	8	64	theme	AMEn	1655:1658	arg1	retention					1627:1635	total tract retention	1615:1635	total tract retention of N, P, AME, and AMEn	1615:1658	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	8	65	theme	total	1555:1559	arg1	retention					1567:1575	total tract retention	1555:1575	total tract retention of Ca	1555:1581	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	12	66	theme	cecal	2022:2026	arg1	SCFAs					2042:2046	cecal saccharolytic SCFAs	2022:2046	(P < 0.01) cecal saccharolytic SCFAs acetate and propionate	2011:2069	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	12	66	theme	cecal	2022:2026	arg1	<					2014:2014	P < 0.01	2012:2019	P < 0.01	2012:2019	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	12	66	theme	cecal	2022:2026	arg1	acetate					2048:2054	acetate	2048:2054	acetate	2048:2054	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	12	66	theme	cecal	2022:2026	arg1	propionate					2060:2069	propionate	2060:2069	propionate	2060:2069	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	4	67	from	maxima	1096:1101	arg1	d					1145:1145	d 15	1145:1148	d 15	1145:1148	Challenged groups were inoculated with a solution containing E. maxima, E. acervulina, and E. tenella oocysts on d 15.					
37354614	12	68	theme	acid	2117:2120	arg1	isovalerate					2122:2132	branched-chain fatty acid isovalerate	2096:2132	branched-chain fatty acid isovalerate	2096:2132	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	1	69	theme	acids	536:540	arg1	expression					475:484	gene expression	470:484	gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp	470:612	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	69	theme	acids	536:540	arg1	performance					387:397	growth performance	380:397	growth performance	380:397	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	18	70	theme	acids	2952:2956	arg1	content					2920:2926	the cecal content	2910:2926	the cecal content of branched-chain fatty acids	2910:2956	All the additives decreased the cecal content of branched-chain fatty acids, consistent with decreased cecal N concentration, although the effects were more pronounced in unchallenged birds.					
37354614	19	71	theme	Eimeria-induced	3126:3140	arg1	perturbation					3152:3163	the Eimeria-induced microbial perturbation	3122:3163	the Eimeria-induced microbial perturbation	3122:3163	In addition, none of the feed additives impacted the Eimeria-induced microbial perturbation.					
37354614	1	72	theme	21-day	274:279	arg1	experiment					281:290	a 21-day experiment	272:290	a 21-day experiment	272:290	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	18	73	theme	fatty	2946:2950	arg1	acids					2952:2956	branched-chain fatty acids	2931:2956	branched-chain fatty acids	2931:2956	All the additives decreased the cecal content of branched-chain fatty acids, consistent with decreased cecal N concentration, although the effects were more pronounced in unchallenged birds.					
37354614	18	74	theme	branched-chain	2931:2944	arg1	acids					2952:2956	branched-chain fatty acids	2931:2956	branched-chain fatty acids	2931:2956	All the additives decreased the cecal content of branched-chain fatty acids, consistent with decreased cecal N concentration, although the effects were more pronounced in unchallenged birds.					
37354614	4	75	theme	E.	1093:1094	arg1	maxima					1096:1101	E. maxima	1093:1101	E. maxima	1093:1101	Challenged groups were inoculated with a solution containing E. maxima, E. acervulina, and E. tenella oocysts on d 15.					
37354614	2	76	theme	g/kg	885:888	arg1	xylo-oligosaccharides					890:910	0.5 g/kg xylo-oligosaccharides	881:910	0.5 g/kg xylo-oligosaccharides	881:910	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	6	77	dep	improved	1247:1254	arg1	<					1259:1259	P < 0.05	1257:1264	P < 0.05	1257:1264	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	9	78	theme	GLUT5	1712:1716	arg1	<					1682:1682	P < 0.05	1680:1687	P < 0.05	1680:1687	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	9	78	theme	GLUT5	1712:1716	arg1	expressions					1690:1700	expressions	1690:1700	(P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1	1679:1768	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	5	79	theme	P	1170:1170	arg1	<					1172:1172	P < 0.01	1170:1177	P < 0.01	1170:1177	Eimeria depressed (P < 0.01) growth performance and nutrient utilization.					
37354614	8	80	theme	XOS	1524:1526	arg1	supplementations					1490:1505	The supplementations	1486:1505	The supplementations of either PRO or XOS	1486:1526	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	15	81	theme	certain	2588:2594	arg1	torques					2627:2633	Ruminococcus torques	2614:2633	Ruminococcus torques	2614:2633	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	15	81	theme	certain	2588:2594	arg1	bacteria					2596:2603	certain bacteria	2588:2603	certain bacteria	2588:2603	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	0	82	from	acids	124:128	arg1	chickens					176:183	Eimeria-challenged broiler chickens	149:183	Eimeria-challenged broiler chickens	149:183	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	15	83	dep	significantly	2386:2398	arg1	<					2403:2403	P < 0.05	2401:2408	P < 0.05	2401:2408	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	9	84	theme	PepT1	1719:1723	arg1	<					1682:1682	P < 0.05	1680:1687	P < 0.05	1680:1687	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	9	84	theme	PepT1	1719:1723	arg1	expressions					1690:1700	expressions	1690:1700	(P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1	1679:1768	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	1	85	theme	Cobb	222:225	arg1	chicks					252:257	392 Cobb 500 off-sex male broiler chicks	218:257	392 Cobb 500 off-sex male broiler chicks	218:257	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	15	86	theme	P	2401:2401	arg1	<					2403:2403	P < 0.05	2401:2408	P < 0.05	2401:2408	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	9	87	theme	ATP2B1	1726:1731	arg1	<					1682:1682	P < 0.05	1680:1687	P < 0.05	1680:1687	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	9	87	theme	ATP2B1	1726:1731	arg1	expressions					1690:1700	expressions	1690:1700	(P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1	1679:1768	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	1	88	theme	nutrient	489:496	arg1	transporters					498:509	nutrient transporters	489:509	nutrient transporters	489:509	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	89	theme	off-sex	231:237	arg1	chicks					252:257	392 Cobb 500 off-sex male broiler chicks	218:257	392 Cobb 500 off-sex male broiler chicks	218:257	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	9	90	theme	GLUT2	1705:1709	arg1	<					1682:1682	P < 0.05	1680:1687	P < 0.05	1680:1687	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	9	90	theme	GLUT2	1705:1709	arg1	expressions					1690:1700	expressions	1690:1700	(P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1	1679:1768	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	4	91	theme	tenella	1126:1132	arg1	oocysts					1134:1140	E. tenella oocysts	1123:1140	E. tenella oocysts on d 15	1123:1148	Challenged groups were inoculated with a solution containing E. maxima, E. acervulina, and E. tenella oocysts on d 15.					
37354614	0	92	theme	low-protein	189:199	arg1	diet					201:204	low-protein diet	189:204	low-protein diet	189:204	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	1	93	theme	nutrient	400:407	arg1	utilization					409:419	nutrient utilization	400:419	nutrient utilization (ileal digestibility and total tract retention)	400:467	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	93	theme	nutrient	400:407	arg1	performance					387:397	growth performance	380:397	growth performance	380:397	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	94	theme	broiler	244:250	arg1	chicks					252:257	392 Cobb 500 off-sex male broiler chicks	218:257	392 Cobb 500 off-sex male broiler chicks	218:257	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	3	95	theme	crude	1006:1010	arg1	protein					1012:1018	crude protein	1006:1018	crude protein (183 g/kg)	1006:1029	The 4 diets were formulated to be marginally low in crude protein (183 g/kg).					
37354614	3	95	theme	crude	1006:1010	arg1	g/kg					1025:1028	183 g/kg	1021:1028	183 g/kg	1021:1028	The 4 diets were formulated to be marginally low in crude protein (183 g/kg).					
37354614	3	96	from	protein	1012:1018	arg1	low					999:1001	low	999:1001	low	999:1001	The 4 diets were formulated to be marginally low in crude protein (183 g/kg).					
37354614	3	96	from	protein	1012:1018	arg1	diets					960:964	The 4 diets	954:964	The 4 diets	954:964	The 4 diets were formulated to be marginally low in crude protein (183 g/kg).					
37354614	15	97	theme	Observed	2445:2452	arg1	richness					2435:2442	the microbial richness	2421:2442	the microbial richness (Observed features)	2421:2462	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	15	97	theme	Observed	2445:2452	arg1	features					2454:2461	Observed features	2445:2461	Observed features	2445:2461	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	2	98	theme	g/kg	764:767	arg1	protease					769:776	0.2 g/kg protease	760:776	0.2 g/kg protease alone (PRO)	760:788	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	2	98	theme	g/kg	764:767	arg1	PRO					785:787	PRO	785:787	PRO	785:787	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	9	99	theme	Calbidin	1740:1747	arg1	D28K					1749:1752	Calbidin D28K	1740:1752	Calbidin D28K	1740:1752	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	11	100	dep	decreased	1885:1893	arg1	<					1898:1898	P < 0.05	1896:1903	P < 0.05	1896:1903	Protease decreased (P < 0.05) isobutyrate concentration in unchallenged treatments but not in challenged treatments.					
37354614	13	101	theme	cecal	2224:2228	arg1	concentrations					2242:2255	decreased cecal isobutyrate concentrations	2214:2255	decreased cecal isobutyrate concentrations	2214:2255	The supplementation of PRO + XYL or XOS increased (P < 0.05) cecal butyrate or decreased cecal isobutyrate concentrations, respectively.					
37354614	1	102	used	used	264:267	arg2	total					209:213	A total	207:213	A total of 392 Cobb 500 off-sex male broiler chicks	207:257	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	2	103	with	diet	721:724	arg1	enzyme					734:739	no enzyme	731:739	no enzyme (Con)	731:745	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	2	103	with	diet	721:724	arg1	Con					742:744	Con	742:744	Con	742:744	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	18	104	theme	unchallenged	3053:3064	arg1	birds					3066:3070	unchallenged birds	3053:3070	unchallenged birds	3053:3070	All the additives decreased the cecal content of branched-chain fatty acids, consistent with decreased cecal N concentration, although the effects were more pronounced in unchallenged birds.					
37354614	0	105	theme	fatty	118:122	arg1	acids					124:128	short-chain fatty acids	106:128	short-chain fatty acids	106:128	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	1	106	theme	total	446:450	arg1	retention					458:466	total tract retention	446:466	total tract retention	446:466	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	6	107	theme	weight	1272:1277	arg1	gain					1279:1282	body weight gain	1267:1282	body weight gain	1267:1282	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	0	108	from	performance	71:81	arg1	chickens					176:183	Eimeria-challenged broiler chickens	149:183	Eimeria-challenged broiler chickens	149:183	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	2	109	theme	corn-soybean	703:714	arg1	diet					721:724	1) corn-soybean meal diet	700:724	1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge	700:951	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	2	110	from	treatments	655:664	arg1	arrangement					687:697	a 4 × 2 factorial arrangement	669:697	a 4 × 2 factorial arrangement	669:697	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	9	111	theme	ZnT1	1765:1768	arg1	<					1682:1682	P < 0.05	1680:1687	P < 0.05	1680:1687	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	9	111	theme	ZnT1	1765:1768	arg1	expressions					1690:1700	expressions	1690:1700	(P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1	1679:1768	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	15	112	theme	Ruminococcus	2614:2625	arg1	torques					2627:2633	Ruminococcus torques	2614:2633	Ruminococcus torques	2614:2633	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	4	113	contain	containing	1082:1091	arg2	oocysts					1134:1140	E. tenella oocysts	1123:1140	E. tenella oocysts on d 15	1123:1148	Challenged groups were inoculated with a solution containing E. maxima, E. acervulina, and E. tenella oocysts on d 15.					
37354614	4	113	contain	containing	1082:1091	arg2	maxima					1096:1101	E. maxima	1093:1101	E. maxima	1093:1101	Challenged groups were inoculated with a solution containing E. maxima, E. acervulina, and E. tenella oocysts on d 15.					
37354614	4	113	contain	containing	1082:1091	arg1	solution					1073:1080	a solution	1071:1080	a solution containing E. maxima, E. acervulina, and E. tenella oocysts on d 15	1071:1148	Challenged groups were inoculated with a solution containing E. maxima, E. acervulina, and E. tenella oocysts on d 15.					
37354614	4	113	contain	containing	1082:1091	arg2	acervulina					1107:1116	acervulina	1107:1116	acervulina	1107:1116	Challenged groups were inoculated with a solution containing E. maxima, E. acervulina, and E. tenella oocysts on d 15.					
37354614	8	114	theme	AME	1646:1648	arg1	retention					1627:1635	total tract retention	1615:1635	total tract retention of N, P, AME, and AMEn	1615:1658	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	8	115	theme	total	1615:1619	arg1	retention					1627:1635	total tract retention	1615:1635	total tract retention of N, P, AME, and AMEn	1615:1658	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	13	116	dep	increased	2175:2183	arg1	<					2188:2188	P < 0.05	2186:2193	P < 0.05	2186:2193	The supplementation of PRO + XYL or XOS increased (P < 0.05) cecal butyrate or decreased cecal isobutyrate concentrations, respectively.					
37354614	15	117	theme	Shannon	2479:2485	arg1	index					2487:2491	Shannon index	2479:2491	Shannon index	2479:2491	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	5	118	theme	growth	1180:1185	arg1	performance					1187:1197	growth performance	1180:1197	growth performance	1180:1197	Eimeria depressed (P < 0.01) growth performance and nutrient utilization.					
37354614	0	119	theme	Eimeria-challenged	149:166	arg1	chickens					176:183	Eimeria-challenged broiler chickens	149:183	Eimeria-challenged broiler chickens	149:183	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	18	120	from	pronounced	3039:3048	arg1	birds					3066:3070	unchallenged birds	3053:3070	unchallenged birds	3053:3070	All the additives decreased the cecal content of branched-chain fatty acids, consistent with decreased cecal N concentration, although the effects were more pronounced in unchallenged birds.					
37354614	6	121	theme	d	1327:1327	arg1	phase					1320:1324	the prechallenge phase	1303:1324	the prechallenge phase (d 0-15)	1303:1333	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	6	121	theme	d	1327:1327	arg1	0-15					1329:1332	d 0-15	1327:1332	d 0-15	1327:1332	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	2	122	theme	4 × 2	671:675	arg1	arrangement					687:697	a 4 × 2 factorial arrangement	669:697	a 4 × 2 factorial arrangement	669:697	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	13	123	theme	cecal	2196:2200	arg1	butyrate					2202:2209	cecal butyrate	2196:2209	cecal butyrate	2196:2209	The supplementation of PRO + XYL or XOS increased (P < 0.05) cecal butyrate or decreased cecal isobutyrate concentrations, respectively.					
37354614	17	124	theme	nutrient	2860:2867	arg1	utilization					2869:2879	nutrient utilization	2860:2879	nutrient utilization	2860:2879	In conclusion, PRO or XOS supplementation individually improved nutrient utilization.					
37354614	8	125	theme	N	1640:1640	arg1	retention					1627:1635	total tract retention	1615:1635	total tract retention of N, P, AME, and AMEn	1615:1658	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	14	126	theme	protein	2306:2312	arg1	levels					2314:2319	cecal protein levels	2300:2319	cecal protein levels	2300:2319	PRO + XYL and XOS decreased cecal protein levels in unchallenged birds but not challenged ones.					
37354614	1	127	theme	profile	566:572	arg1	expression					475:484	gene expression	470:484	gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp	470:612	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	127	theme	profile	566:572	arg1	performance					387:397	growth performance	380:397	growth performance	380:397	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	8	128	theme	P	1643:1643	arg1	retention					1627:1635	total tract retention	1615:1635	total tract retention of N, P, AME, and AMEn	1615:1658	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	16	129	theme	microbial	2773:2781	arg1	composition					2783:2793	the cecal microbial composition	2763:2793	the cecal microbial composition	2763:2793	In contrast, none of the additives had any significant effect on the cecal microbial composition.					
37354614	7	130	theme	feed	1439:1442	arg1	supplementation					1444:1458	feed supplementation	1439:1458	feed supplementation	1439:1458	There was no interaction between infection and feed supplementation for nutrient utilization.					
37354614	15	131	theme	others	2668:2673	arg1	abundance					2655:2663	the abundance	2651:2663	the abundance of others, such as Anaerostipes	2651:2695	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	13	132	theme	isobutyrate	2230:2240	arg1	concentrations					2242:2255	decreased cecal isobutyrate concentrations	2214:2255	decreased cecal isobutyrate concentrations	2214:2255	The supplementation of PRO + XYL or XOS increased (P < 0.05) cecal butyrate or decreased cecal isobutyrate concentrations, respectively.					
37354614	0	133	from	effects	4:10	arg1	acids					124:128	short-chain fatty acids	106:128	short-chain fatty acids	106:128	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	0	133	from	effects	4:10	arg1	microbiota					135:144	microbiota	135:144	microbiota in Eimeria-challenged broiler chickens	135:183	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	0	133	from	effects	4:10	arg1	performance					71:81	growth performance	64:81	growth performance	64:81	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	0	133	from	effects	4:10	arg1	utilization					93:103	nutrient utilization	84:103	nutrient utilization	84:103	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	1	134	theme	broilers	577:584	arg1	expression					475:484	gene expression	470:484	gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp	470:612	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	134	theme	broilers	577:584	arg1	performance					387:397	growth performance	380:397	growth performance	380:397	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	9	135	theme	GLUT1	1809:1813	arg1	expression					1795:1804	expression	1795:1804	expression of GLUT1	1795:1813	Eimeria decreased (P < 0.05) expressions of GLUT2, GLUT5, PepT1, ATP2B1, CaSR, Calbidin D28K, NPT2, and ZnT1 but increased (P < 0.01) expression of GLUT1.					
37354614	0	136	theme	nutrient	84:91	arg1	utilization					93:103	nutrient utilization	84:103	nutrient utilization	84:103	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	1	137	theme	protease	315:322	arg1	effect					305:310	the effect	301:310	the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp	301:612	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	8	138	theme	P	1545:1545	arg1	<					1547:1547	P < 0.01	1545:1552	P < 0.01	1545:1552	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	2	139	theme	Eimeria	935:941	arg1	challenge					943:951	Eimeria challenge	935:951	Eimeria challenge	935:951	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	1	140	theme	transporters	498:509	arg1	expression					475:484	gene expression	470:484	gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp	470:612	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	140	theme	transporters	498:509	arg1	performance					387:397	growth performance	380:397	growth performance	380:397	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	13	141	theme	XOS	2171:2173	arg1	supplementation					2139:2153	The supplementation	2135:2153	The supplementation of PRO + XYL or XOS	2135:2173	The supplementation of PRO + XYL or XOS increased (P < 0.05) cecal butyrate or decreased cecal isobutyrate concentrations, respectively.					
37354614	1	142	theme	xylanase	325:332	arg1	effect					305:310	the effect	301:310	the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp	301:612	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	14	143	theme	cecal	2300:2304	arg1	levels					2314:2319	cecal protein levels	2300:2319	cecal protein levels	2300:2319	PRO + XYL and XOS decreased cecal protein levels in unchallenged birds but not challenged ones.					
37354614	18	144	theme	cecal	2985:2989	arg1	concentration					2993:3005	decreased cecal N concentration	2975:3005	decreased cecal N concentration	2975:3005	All the additives decreased the cecal content of branched-chain fatty acids, consistent with decreased cecal N concentration, although the effects were more pronounced in unchallenged birds.					
37354614	10	145	theme	XOS	1816:1818	arg1	supplementation					1820:1834	XOS supplementation	1816:1834	XOS supplementation	1816:1834	XOS supplementation increased (P < 0.05) ATP2B1 expression.					
37354614	1	146	theme	cecal	512:516	arg1	acids					536:540	cecal short-chain fatty acids	512:540	cecal short-chain fatty acids (SCFAs)	512:548	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	146	theme	cecal	512:516	arg1	SCFAs					543:547	SCFAs	543:547	SCFAs	543:547	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	5	147	dep	depressed	1159:1167	arg1	<					1172:1172	P < 0.01	1170:1177	P < 0.01	1170:1177	Eimeria depressed (P < 0.01) growth performance and nutrient utilization.					
37354614	16	148	contain	had	2733:2735	arg1	none					2711:2714	none	2711:2714	none of the additives	2711:2731	In contrast, none of the additives had any significant effect on the cecal microbial composition.					
37354614	16	148	contain	had	2733:2735	arg2	effect					2753:2758	any significant effect	2737:2758	any significant effect	2737:2758	In contrast, none of the additives had any significant effect on the cecal microbial composition.					
37354614	11	149	theme	unchallenged	1935:1946	arg1	treatments					1948:1957	unchallenged treatments	1935:1957	unchallenged treatments	1935:1957	Protease decreased (P < 0.05) isobutyrate concentration in unchallenged treatments but not in challenged treatments.					
37354614	1	150	theme	fatty	530:534	arg1	acids					536:540	cecal short-chain fatty acids	512:540	cecal short-chain fatty acids (SCFAs)	512:548	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	150	theme	fatty	530:534	arg1	SCFAs					543:547	SCFAs	543:547	SCFAs	543:547	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	19	151	theme	additives	3103:3111	arg1	none					3086:3089	none	3086:3089	none of the feed additives	3086:3111	In addition, none of the feed additives impacted the Eimeria-induced microbial perturbation.					
37354614	0	152	theme	protease	15:22	arg1	effects					4:10	The effects	0:10	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens	0:183	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	11	153	theme	isobutyrate	1906:1916	arg1	concentration					1918:1930	isobutyrate concentration	1906:1930	isobutyrate concentration	1906:1930	Protease decreased (P < 0.05) isobutyrate concentration in unchallenged treatments but not in challenged treatments.					
37354614	8	154	dep	improve	1607:1613	arg1	P					1595:1595	P	1595:1595	P < 0.1	1595:1601	The supplementations of either PRO or XOS alone increased (P < 0.01) total tract retention of Ca and tended (P < 0.1) to improve total tract retention of N, P, AME, and AMEn.					
37354614	12	155	theme	P	2012:2012	arg1	SCFAs					2042:2046	cecal saccharolytic SCFAs	2022:2046	(P < 0.01) cecal saccharolytic SCFAs acetate and propionate	2011:2069	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	12	155	theme	P	2012:2012	arg1	<					2014:2014	P < 0.01	2012:2019	P < 0.01	2012:2019	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	12	156	theme	branched-chain	2096:2109	arg1	acid					2117:2120	branched-chain fatty acid	2096:2120	branched-chain fatty acid isovalerate	2096:2132	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	17	157	theme	XOS	2818:2820	arg1	supplementation					2822:2836	PRO or XOS supplementation	2811:2836	PRO or XOS supplementation	2811:2836	In conclusion, PRO or XOS supplementation individually improved nutrient utilization.					
37354614	0	158	theme	xylanase	25:32	arg1	effects					4:10	The effects	0:10	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens	0:183	The effects of protease, xylanase, and xylo-oligosaccharides on growth performance, nutrient utilization, short-chain fatty acids, and microbiota in Eimeria-challenged broiler chickens fed low-protein diet.					
37354614	3	159	from	low	999:1001	arg1	protein					1012:1018	crude protein	1006:1018	crude protein (183 g/kg)	1006:1029	The 4 diets were formulated to be marginally low in crude protein (183 g/kg).					
37354614	3	159	from	low	999:1001	arg1	g/kg					1025:1028	183 g/kg	1021:1028	183 g/kg	1021:1028	The 4 diets were formulated to be marginally low in crude protein (183 g/kg).					
37354614	13	160	theme	PRO + XYL	2158:2166	arg1	supplementation					2139:2153	The supplementation	2135:2153	The supplementation of PRO + XYL or XOS	2135:2173	The supplementation of PRO + XYL or XOS increased (P < 0.05) cecal butyrate or decreased cecal isobutyrate concentrations, respectively.					
37354614	15	161	theme	phylogenetic	2497:2508	arg1	diversity					2510:2518	phylogenetic diversity	2497:2518	phylogenetic diversity	2497:2518	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	15	162	theme	microbial	2537:2545	arg1	composition					2547:2557	the microbial composition	2533:2557	the microbial composition	2533:2557	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	1	163	theme	tract	452:456	arg1	retention					458:466	total tract retention	446:466	total tract retention	446:466	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	19	164	theme	microbial	3142:3150	arg1	perturbation					3152:3163	the Eimeria-induced microbial perturbation	3122:3163	the Eimeria-induced microbial perturbation	3122:3163	In addition, none of the feed additives impacted the Eimeria-induced microbial perturbation.					
37354614	15	165	theme	bacteria	2596:2603	arg1	abundance					2575:2583	the abundance	2571:2583	the abundance of certain bacteria, such as Ruminococcus torques	2571:2633	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	2	166	dep	Con	794:796	arg1	3					791:791	3	791:791	3	791:791	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	2	167	dep	Con	751:753	arg1	2					748:748	2	748:748	2	748:748	Chicks at 0-day old were allocated to 8 treatments in a 4 × 2 factorial arrangement: 1) corn-soybean meal diet with no enzyme (Con); 2) Con plus 0.2 g/kg protease alone (PRO); 3) Con plus 0.2 g/kg protease combined with 0.1 g/kg xylanase (PRO + XYL); or 4) Con plus 0.5 g/kg xylo-oligosaccharides (XOS); with or without Eimeria challenge.					
37354614	12	168	theme	fatty	2111:2115	arg1	acid					2117:2120	branched-chain fatty acid	2096:2120	branched-chain fatty acid isovalerate	2096:2132	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	12	169	theme	P	2086:2086	arg1	<					2088:2088	P < 0.01	2086:2093	P < 0.01	2086:2093	Eimeria decreased (P < 0.01) cecal saccharolytic SCFAs acetate and propionate but increased (P < 0.01) branched-chain fatty acid isovalerate.					
37354614	1	170	theme	gene	470:473	arg1	expression					475:484	gene expression	470:484	gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp	470:612	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	1	170	theme	gene	470:473	arg1	performance					387:397	growth performance	380:397	growth performance	380:397	A total of 392 Cobb 500 off-sex male broiler chicks were used in a 21-day experiment to study the effect of protease, xylanase, and xylo-oligosaccharides (XOS) on improving growth performance, nutrient utilization (ileal digestibility and total tract retention), gene expression of nutrient transporters, cecal short-chain fatty acids (SCFAs), and microbiota profile of broilers challenged with Eimeria spp.					
37354614	6	171	theme	P	1257:1257	arg1	<					1259:1259	P < 0.05	1257:1264	P < 0.05	1257:1264	Supplemental protease improved (P < 0.05) body weight gain and feed intake in the prechallenge phase (d 0-15) but had no effect during the infection period (d 15-21).					
37354614	15	172	dep	diversity	2468:2476	arg1	index					2487:2491	Shannon index	2479:2491	Shannon index	2479:2491	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	15	172	dep	diversity	2468:2476	arg1	diversity					2510:2518	phylogenetic diversity	2497:2518	phylogenetic diversity	2497:2518	Eimeria challenge significantly (P < 0.05) decreased the microbial richness (Observed features) and diversity (Shannon index and phylogenetic diversity) and changed the microbial composition by reducing the abundance of certain bacteria, such as Ruminococcus torques, and increasing the abundance of others, such as Anaerostipes.					
37354614	4	173	theme	E.	1123:1124	arg1	oocysts					1134:1140	E. tenella oocysts	1123:1140	E. tenella oocysts on d 15	1123:1148	Challenged groups were inoculated with a solution containing E. maxima, E. acervulina, and E. tenella oocysts on d 15.					
37247711	0	0	theme	anti-bacterial	91:104	arg1	hydrogel					106:113	an anti-bacterial hydrogel	88:113	an anti-bacterial hydrogel in co-operation	88:129	Selective preparation of crystalline or fibrous nano-cellulose carboxylate to fabricate an anti-bacterial hydrogel in co-operation with ZnO and recycled gelatin.					
37247711	5	1	theme	cellulose	1076:1084	arg1	hydroxyls					1086:1094	cellulose hydroxyls	1076:1094	cellulose hydroxyls	1076:1094	Nano-ZnO plays multi-roles in this hydrogel preparation, as either catalyst for the esterification of cellulose hydroxyls and amidation of gelatin amino groups or as the anti-bacterial part of hydrogel.					
37247711	4	2	theme	gelatin	830:836	arg1	Gel					839:841	the recycled gelatin (Gel)	817:842	the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO	817:971	Then, NCCC was cross-linked to the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO.					
37247711	3	3	theme	thermal	702:708	arg1	TGA					732:734	TGA	732:734	TGA	732:734	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	3	3	theme	thermal	702:708	arg1	analysis					722:729	thermal gravimetric analysis	702:729	thermal gravimetric analysis (TGA)	702:735	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	1	4	theme	based	176:180	arg1	hydrogels					202:210	hydrogels	202:210	hydrogels	202:210	Bio-polymeric based nano-composites and hydrogels are newsworthy nano-biomaterials.					
37247711	1	4	theme	based	176:180	arg1	nano-biomaterials					227:243	newsworthy nano-biomaterials	216:243	newsworthy nano-biomaterials	216:243	Bio-polymeric based nano-composites and hydrogels are newsworthy nano-biomaterials.					
37247711	1	4	theme	based	176:180	arg1	nano-composites					182:196	Bio-polymeric based nano-composites	162:196	Bio-polymeric based nano-composites	162:196	Bio-polymeric based nano-composites and hydrogels are newsworthy nano-biomaterials.					
37247711	5	5	theme	anti-bacterial	1144:1157	arg1	part					1159:1162	the anti-bacterial part	1140:1162	the anti-bacterial part of hydrogel	1140:1174	Nano-ZnO plays multi-roles in this hydrogel preparation, as either catalyst for the esterification of cellulose hydroxyls and amidation of gelatin amino groups or as the anti-bacterial part of hydrogel.					
37247711	7	6	theme	gram-negative	1488:1500	arg1	bacteria					1512:1519	the gram-negative resistant bacteria	1484:1519	the gram-negative resistant bacteria of P. aeruginosa	1484:1536	The top anti-bacterial activity of this hydrogel against the gram-negative resistant bacteria of P. aeruginosa suggests its potential for biomedical applications.					
37247711	5	7	theme	hydroxyls	1086:1094	arg1	esterification					1058:1071	the esterification	1054:1071	the esterification of cellulose hydroxyls	1054:1094	Nano-ZnO plays multi-roles in this hydrogel preparation, as either catalyst for the esterification of cellulose hydroxyls and amidation of gelatin amino groups or as the anti-bacterial part of hydrogel.					
37247711	4	8	theme	recycled	821:828	arg1	Gel					839:841	the recycled gelatin (Gel)	817:842	the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO	817:971	Then, NCCC was cross-linked to the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO.					
37247711	2	9	from	oxidative-hydrolysis	374:393	arg1	NaClO2					413:418	alkaline NaClO2	404:418	alkaline NaClO2	404:418	Herein, crystalline or fibrous nano-cellulose carboxylate (NCCC and NCCF) were selectively prepared via the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2 at 1:2 mol ratio, 90 °C, and 24 h for NCCC and at 1:1 mol ratio, 70 °C, and 20 h for NCCF.					
37247711	3	10	dep	transform	583:591	arg1	infrared					593:600	infrared	593:600	transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS)	583:783	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	6	11	dep	in	1181:1182	arg1	vitro					1184:1188	vitro	1184:1188	vitro	1184:1188	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method confirmed Gel/MA/NCCC/nano-ZnO as an antibacterial agent with the activity order of P. aeruginosa > S. aureus > E. coli.					
37247711	6	12	theme	antibacterial	1343:1355	arg1	agent					1357:1361	an antibacterial agent	1340:1361	an antibacterial agent	1340:1361	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method confirmed Gel/MA/NCCC/nano-ZnO as an antibacterial agent with the activity order of P. aeruginosa > S. aureus > E. coli.					
37247711	6	12	theme	antibacterial	1343:1355	arg1	Gel/MA/NCCC/nano-ZnO					1316:1335	Gel/MA/NCCC/nano-ZnO	1316:1335	Gel/MA/NCCC/nano-ZnO	1316:1335	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method confirmed Gel/MA/NCCC/nano-ZnO as an antibacterial agent with the activity order of P. aeruginosa > S. aureus > E. coli.					
37247711	3	13	theme	gravimetric	710:720	arg1	TGA					732:734	TGA	732:734	TGA	732:734	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	3	13	theme	gravimetric	710:720	arg1	analysis					722:729	thermal gravimetric analysis	702:729	thermal gravimetric analysis (TGA)	702:735	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	7	14	theme	top	1431:1433	arg1	activity					1450:1457	The top anti-bacterial activity	1427:1457	The top anti-bacterial activity of this hydrogel against the gram-negative resistant bacteria of P. aeruginosa	1427:1536	The top anti-bacterial activity of this hydrogel against the gram-negative resistant bacteria of P. aeruginosa suggests its potential for biomedical applications.					
37247711	2	15	theme	alkaline	404:411	arg1	NaClO2					413:418	alkaline NaClO2	404:418	alkaline NaClO2	404:418	Herein, crystalline or fibrous nano-cellulose carboxylate (NCCC and NCCF) were selectively prepared via the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2 at 1:2 mol ratio, 90 °C, and 24 h for NCCC and at 1:1 mol ratio, 70 °C, and 20 h for NCCF.					
37247711	4	16	from	nano-ZnO	891:898	arg1	Gel					839:841	the recycled gelatin (Gel)	817:842	the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO	817:971	Then, NCCC was cross-linked to the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO.					
37247711	5	17	theme	hydrogel	1167:1174	arg1	part					1159:1162	the anti-bacterial part	1140:1162	the anti-bacterial part of hydrogel	1140:1174	Nano-ZnO plays multi-roles in this hydrogel preparation, as either catalyst for the esterification of cellulose hydroxyls and amidation of gelatin amino groups or as the anti-bacterial part of hydrogel.					
37247711	4	18	from	capsules	862:869	arg1	Gel					839:841	the recycled gelatin (Gel)	817:842	the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO	817:971	Then, NCCC was cross-linked to the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO.					
37247711	4	19	theme	novel	937:941	arg1	Gel/MA/NCCC/nano-ZnO					952:971	the novel hydrogel Gel/MA/NCCC/nano-ZnO	933:971	the novel hydrogel Gel/MA/NCCC/nano-ZnO	933:971	Then, NCCC was cross-linked to the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO.					
37247711	7	20	theme	anti-bacterial	1435:1448	arg1	activity					1450:1457	The top anti-bacterial activity	1427:1457	The top anti-bacterial activity of this hydrogel against the gram-negative resistant bacteria of P. aeruginosa	1427:1536	The top anti-bacterial activity of this hydrogel against the gram-negative resistant bacteria of P. aeruginosa suggests its potential for biomedical applications.					
37247711	3	21	theme	NCCF	540:543	arg1	Characterization					511:526	Characterization	511:526	Characterization of NCCC and NCCF	511:543	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	2	22	dep	carboxylate	292:302	arg1	NCCF					314:317	NCCF	314:317	NCCF	314:317	Herein, crystalline or fibrous nano-cellulose carboxylate (NCCC and NCCF) were selectively prepared via the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2 at 1:2 mol ratio, 90 °C, and 24 h for NCCC and at 1:1 mol ratio, 70 °C, and 20 h for NCCF.					
37247711	2	22	dep	carboxylate	292:302	arg1	NCCC					305:308	NCCC	305:308	NCCC	305:308	Herein, crystalline or fibrous nano-cellulose carboxylate (NCCC and NCCF) were selectively prepared via the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2 at 1:2 mol ratio, 90 °C, and 24 h for NCCC and at 1:1 mol ratio, 70 °C, and 20 h for NCCF.					
37247711	2	23	theme	1:1 mol	470:476	arg1	ratio					478:482	1:1 mol ratio	470:482	1:1 mol ratio	470:482	Herein, crystalline or fibrous nano-cellulose carboxylate (NCCC and NCCF) were selectively prepared via the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2 at 1:2 mol ratio, 90 °C, and 24 h for NCCC and at 1:1 mol ratio, 70 °C, and 20 h for NCCF.					
37247711	3	24	theme	NCCC	531:534	arg1	Characterization					511:526	Characterization	511:526	Characterization of NCCC and NCCF	511:543	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	6	25	theme	activity	1205:1212	arg1	results					1214:1220	The in vitro anti-bacterial activity results	1177:1220	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method	1177:1304	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method confirmed Gel/MA/NCCC/nano-ZnO as an antibacterial agent with the activity order of P. aeruginosa > S. aureus > E. coli.					
37247711	2	26	theme	controllable	354:365	arg1	oxidative-hydrolysis					374:393	the controllable direct oxidative-hydrolysis	350:393	the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2	350:418	Herein, crystalline or fibrous nano-cellulose carboxylate (NCCC and NCCF) were selectively prepared via the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2 at 1:2 mol ratio, 90 °C, and 24 h for NCCC and at 1:1 mol ratio, 70 °C, and 20 h for NCCF.					
37247711	5	27	theme	amino	1121:1125	arg1	groups					1127:1132	gelatin amino groups	1113:1132	gelatin amino groups	1113:1132	Nano-ZnO plays multi-roles in this hydrogel preparation, as either catalyst for the esterification of cellulose hydroxyls and amidation of gelatin amino groups or as the anti-bacterial part of hydrogel.					
37247711	0	28	theme	Selective	0:8	arg1	preparation					10:20	Selective preparation	0:20	Selective preparation of crystalline or fibrous nano-cellulose	0:61	Selective preparation of crystalline or fibrous nano-cellulose carboxylate to fabricate an anti-bacterial hydrogel in co-operation with ZnO and recycled gelatin.					
37247711	5	29	theme	groups	1127:1132	arg1	amidation					1100:1108	amidation	1100:1108	amidation of gelatin amino groups	1100:1132	Nano-ZnO plays multi-roles in this hydrogel preparation, as either catalyst for the esterification of cellulose hydroxyls and amidation of gelatin amino groups or as the anti-bacterial part of hydrogel.					
37247711	5	30	theme	hydrogel	1009:1016	arg1	preparation					1018:1028	this hydrogel preparation	1004:1028	this hydrogel preparation	1004:1028	Nano-ZnO plays multi-roles in this hydrogel preparation, as either catalyst for the esterification of cellulose hydroxyls and amidation of gelatin amino groups or as the anti-bacterial part of hydrogel.					
37247711	2	31	theme	1:2 mol	423:429	arg1	ratio					431:435	1:2 mol ratio	423:435	1:2 mol ratio	423:435	Herein, crystalline or fibrous nano-cellulose carboxylate (NCCC and NCCF) were selectively prepared via the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2 at 1:2 mol ratio, 90 °C, and 24 h for NCCC and at 1:1 mol ratio, 70 °C, and 20 h for NCCF.					
37247711	0	32	theme	crystalline	25:35	arg1	nano-cellulose					48:61	crystalline or fibrous nano-cellulose	25:61	crystalline or fibrous nano-cellulose	25:61	Selective preparation of crystalline or fibrous nano-cellulose carboxylate to fabricate an anti-bacterial hydrogel in co-operation with ZnO and recycled gelatin.					
37247711	6	33	theme	gram-negative	1240:1252	arg1	bacteria					1272:1279	the three gram-negative and gram-positive bacteria	1230:1279	the three gram-negative and gram-positive bacteria by well diffusion method	1230:1304	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method confirmed Gel/MA/NCCC/nano-ZnO as an antibacterial agent with the activity order of P. aeruginosa > S. aureus > E. coli.					
37247711	2	34	theme	nano-cellulose	277:290	arg1	carboxylate					292:302	fibrous nano-cellulose carboxylate	269:302	fibrous nano-cellulose carboxylate (NCCC and NCCF)	269:318	Herein, crystalline or fibrous nano-cellulose carboxylate (NCCC and NCCF) were selectively prepared via the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2 at 1:2 mol ratio, 90 °C, and 24 h for NCCC and at 1:1 mol ratio, 70 °C, and 20 h for NCCF.					
37247711	6	35	theme	coli	1421:1424	arg1	order					1381:1385	the activity order	1368:1385	the activity order of P. aeruginosa > S. aureus > E. coli	1368:1424	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method confirmed Gel/MA/NCCC/nano-ZnO as an antibacterial agent with the activity order of P. aeruginosa > S. aureus > E. coli.					
37247711	3	36	theme	comparative	563:573	arg1	Fourier					575:581	comparative Fourier	563:581	comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS)	563:783	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	0	37	theme	fibrous	40:46	arg1	nano-cellulose					48:61	crystalline or fibrous nano-cellulose	25:61	crystalline or fibrous nano-cellulose	25:61	Selective preparation of crystalline or fibrous nano-cellulose carboxylate to fabricate an anti-bacterial hydrogel in co-operation with ZnO and recycled gelatin.					
37247711	4	38	theme	as-prepared	879:889	arg1	nano-ZnO					891:898	the as-prepared nano-ZnO	875:898	the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO	875:971	Then, NCCC was cross-linked to the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO.					
37247711	3	39	theme	FT-IR	603:607	arg1	spectroscopy					610:621	(FT-IR) spectroscopy	602:621	(FT-IR) spectroscopy	602:621	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	1	40	theme	newsworthy	216:225	arg1	hydrogels					202:210	hydrogels	202:210	hydrogels	202:210	Bio-polymeric based nano-composites and hydrogels are newsworthy nano-biomaterials.					
37247711	1	40	theme	newsworthy	216:225	arg1	nano-biomaterials					227:243	newsworthy nano-biomaterials	216:243	newsworthy nano-biomaterials	216:243	Bio-polymeric based nano-composites and hydrogels are newsworthy nano-biomaterials.					
37247711	1	40	theme	newsworthy	216:225	arg1	nano-composites					182:196	Bio-polymeric based nano-composites	162:196	Bio-polymeric based nano-composites	162:196	Bio-polymeric based nano-composites and hydrogels are newsworthy nano-biomaterials.					
37247711	3	41	theme	dispersive	749:758	arg1	EDS					780:782	EDS	780:782	EDS	780:782	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	3	41	theme	dispersive	749:758	arg1	spectroscopy					766:777	energy dispersive X-ray spectroscopy	742:777	energy dispersive X-ray spectroscopy (EDS)	742:783	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	0	42	theme	recycled	144:151	arg1	gelatin					153:159	recycled gelatin	144:159	recycled gelatin	144:159	Selective preparation of crystalline or fibrous nano-cellulose carboxylate to fabricate an anti-bacterial hydrogel in co-operation with ZnO and recycled gelatin.					
37247711	3	43	theme	scanning	639:646	arg1	microscopy					657:666	field emission scanning electron microscopy	624:666	field emission scanning electron microscopy (FESEM)	624:674	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	3	43	theme	scanning	639:646	arg1	FESEM					669:673	FESEM	669:673	FESEM	669:673	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	2	44	theme	fibrous	269:275	arg1	carboxylate					292:302	fibrous nano-cellulose carboxylate	269:302	fibrous nano-cellulose carboxylate (NCCC and NCCF)	269:318	Herein, crystalline or fibrous nano-cellulose carboxylate (NCCC and NCCF) were selectively prepared via the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2 at 1:2 mol ratio, 90 °C, and 24 h for NCCC and at 1:1 mol ratio, 70 °C, and 20 h for NCCF.					
37247711	4	45	theme	maleic	903:908	arg1	MA					921:922	MA	921:922	MA	921:922	Then, NCCC was cross-linked to the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO.					
37247711	4	45	theme	maleic	903:908	arg1	anhydride					910:918	maleic anhydride	903:918	maleic anhydride (MA)	903:923	Then, NCCC was cross-linked to the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO.					
37247711	4	46	theme	hydrogel	943:950	arg1	Gel/MA/NCCC/nano-ZnO					952:971	the novel hydrogel Gel/MA/NCCC/nano-ZnO	933:971	the novel hydrogel Gel/MA/NCCC/nano-ZnO	933:971	Then, NCCC was cross-linked to the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO.					
37247711	3	47	theme	X-ray	760:764	arg1	EDS					780:782	EDS	780:782	EDS	780:782	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	3	47	theme	X-ray	760:764	arg1	spectroscopy					766:777	energy dispersive X-ray spectroscopy	742:777	energy dispersive X-ray spectroscopy (EDS)	742:783	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	6	48	theme	well	1284:1287	arg1	method					1299:1304	well diffusion method	1284:1304	well diffusion method	1284:1304	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method confirmed Gel/MA/NCCC/nano-ZnO as an antibacterial agent with the activity order of P. aeruginosa > S. aureus > E. coli.					
37247711	3	49	theme	electron	648:655	arg1	microscopy					657:666	field emission scanning electron microscopy	624:666	field emission scanning electron microscopy (FESEM)	624:674	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	3	49	theme	electron	648:655	arg1	FESEM					669:673	FESEM	669:673	FESEM	669:673	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	2	50	theme	MC	398:399	arg1	oxidative-hydrolysis					374:393	the controllable direct oxidative-hydrolysis	350:393	the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2	350:418	Herein, crystalline or fibrous nano-cellulose carboxylate (NCCC and NCCF) were selectively prepared via the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2 at 1:2 mol ratio, 90 °C, and 24 h for NCCC and at 1:1 mol ratio, 70 °C, and 20 h for NCCF.					
37247711	0	51	theme	nano-cellulose	48:61	arg1	preparation					10:20	Selective preparation	0:20	Selective preparation of crystalline or fibrous nano-cellulose	0:61	Selective preparation of crystalline or fibrous nano-cellulose carboxylate to fabricate an anti-bacterial hydrogel in co-operation with ZnO and recycled gelatin.					
37247711	5	52	theme	gelatin	1113:1119	arg1	groups					1127:1132	gelatin amino groups	1113:1132	gelatin amino groups	1113:1132	Nano-ZnO plays multi-roles in this hydrogel preparation, as either catalyst for the esterification of cellulose hydroxyls and amidation of gelatin amino groups or as the anti-bacterial part of hydrogel.					
37247711	7	53	theme	aeruginosa	1527:1536	arg1	bacteria					1512:1519	the gram-negative resistant bacteria	1484:1519	the gram-negative resistant bacteria of P. aeruginosa	1484:1536	The top anti-bacterial activity of this hydrogel against the gram-negative resistant bacteria of P. aeruginosa suggests its potential for biomedical applications.					
37247711	3	54	theme	energy	742:747	arg1	EDS					780:782	EDS	780:782	EDS	780:782	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	3	54	theme	energy	742:747	arg1	spectroscopy					766:777	energy dispersive X-ray spectroscopy	742:777	energy dispersive X-ray spectroscopy (EDS)	742:783	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	4	55	theme	medicine	853:860	arg1	capsules					862:869	the medicine capsules	849:869	the medicine capsules	849:869	Then, NCCC was cross-linked to the recycled gelatin (Gel) from the medicine capsules and the as-prepared nano-ZnO by maleic anhydride (MA) to give the novel hydrogel Gel/MA/NCCC/nano-ZnO.					
37247711	3	56	theme	X-ray	677:681	arg1	XRD					696:698	XRD	696:698	XRD	696:698	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	3	56	theme	X-ray	677:681	arg1	diffraction					683:693	X-ray diffraction	677:693	X-ray diffraction (XRD)	677:699	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	3	57	theme	field	624:628	arg1	microscopy					657:666	field emission scanning electron microscopy	624:666	field emission scanning electron microscopy (FESEM)	624:674	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	3	57	theme	field	624:628	arg1	FESEM					669:673	FESEM	669:673	FESEM	669:673	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	6	58	theme	in	1181:1182	arg1	results					1214:1220	The in vitro anti-bacterial activity results	1177:1220	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method	1177:1304	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method confirmed Gel/MA/NCCC/nano-ZnO as an antibacterial agent with the activity order of P. aeruginosa > S. aureus > E. coli.					
37247711	3	59	dep	Fourier	575:581	arg1	transform					583:591	transform	583:591	transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS)	583:783	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	6	60	theme	diffusion	1289:1297	arg1	method					1299:1304	well diffusion method	1284:1304	well diffusion method	1284:1304	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method confirmed Gel/MA/NCCC/nano-ZnO as an antibacterial agent with the activity order of P. aeruginosa > S. aureus > E. coli.					
37247711	6	61	theme	gram-positive	1258:1270	arg1	bacteria					1272:1279	the three gram-negative and gram-positive bacteria	1230:1279	the three gram-negative and gram-positive bacteria by well diffusion method	1230:1304	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method confirmed Gel/MA/NCCC/nano-ZnO as an antibacterial agent with the activity order of P. aeruginosa > S. aureus > E. coli.					
37247711	7	62	theme	resistant	1502:1510	arg1	bacteria					1512:1519	the gram-negative resistant bacteria	1484:1519	the gram-negative resistant bacteria of P. aeruginosa	1484:1536	The top anti-bacterial activity of this hydrogel against the gram-negative resistant bacteria of P. aeruginosa suggests its potential for biomedical applications.					
37247711	3	63	theme	emission	630:637	arg1	microscopy					657:666	field emission scanning electron microscopy	624:666	field emission scanning electron microscopy (FESEM)	624:674	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	3	63	theme	emission	630:637	arg1	FESEM					669:673	FESEM	669:673	FESEM	669:673	Characterization of NCCC and NCCF were performed by comparative Fourier transform infrared (FT-IR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and energy dispersive X-ray spectroscopy (EDS).					
37247711	2	64	theme	direct	367:372	arg1	oxidative-hydrolysis					374:393	the controllable direct oxidative-hydrolysis	350:393	the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2	350:418	Herein, crystalline or fibrous nano-cellulose carboxylate (NCCC and NCCF) were selectively prepared via the controllable direct oxidative-hydrolysis of MC in alkaline NaClO2 at 1:2 mol ratio, 90 °C, and 24 h for NCCC and at 1:1 mol ratio, 70 °C, and 20 h for NCCF.					
37247711	0	65	from	hydrogel	106:113	arg1	co-operation					118:129	co-operation	118:129	co-operation	118:129	Selective preparation of crystalline or fibrous nano-cellulose carboxylate to fabricate an anti-bacterial hydrogel in co-operation with ZnO and recycled gelatin.					
37247711	7	66	theme	biomedical	1565:1574	arg1	applications					1576:1587	biomedical applications	1565:1587	biomedical applications	1565:1587	The top anti-bacterial activity of this hydrogel against the gram-negative resistant bacteria of P. aeruginosa suggests its potential for biomedical applications.					
37247711	7	67	theme	hydrogel	1467:1474	arg1	activity					1450:1457	The top anti-bacterial activity	1427:1457	The top anti-bacterial activity of this hydrogel against the gram-negative resistant bacteria of P. aeruginosa	1427:1536	The top anti-bacterial activity of this hydrogel against the gram-negative resistant bacteria of P. aeruginosa suggests its potential for biomedical applications.					
37247711	6	68	theme	activity	1372:1379	arg1	order					1381:1385	the activity order	1368:1385	the activity order of P. aeruginosa > S. aureus > E. coli	1368:1424	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method confirmed Gel/MA/NCCC/nano-ZnO as an antibacterial agent with the activity order of P. aeruginosa > S. aureus > E. coli.					
37247711	6	69	theme	anti-bacterial	1190:1203	arg1	results					1214:1220	The in vitro anti-bacterial activity results	1177:1220	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method	1177:1304	The in vitro anti-bacterial activity results against the three gram-negative and gram-positive bacteria by well diffusion method confirmed Gel/MA/NCCC/nano-ZnO as an antibacterial agent with the activity order of P. aeruginosa > S. aureus > E. coli.					
37247711	1	70	theme	Bio-polymeric	162:174	arg1	hydrogels					202:210	hydrogels	202:210	hydrogels	202:210	Bio-polymeric based nano-composites and hydrogels are newsworthy nano-biomaterials.					
37247711	1	70	theme	Bio-polymeric	162:174	arg1	nano-biomaterials					227:243	newsworthy nano-biomaterials	216:243	newsworthy nano-biomaterials	216:243	Bio-polymeric based nano-composites and hydrogels are newsworthy nano-biomaterials.					
37247711	1	70	theme	Bio-polymeric	162:174	arg1	nano-composites					182:196	Bio-polymeric based nano-composites	162:196	Bio-polymeric based nano-composites	162:196	Bio-polymeric based nano-composites and hydrogels are newsworthy nano-biomaterials.					
35806282	0	0	theme	Osteoblast	113:122	arg1	Culture					124:130	Osteoblast Culture	113:130	Osteoblast Culture	113:130	Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture.					
35806282	2	1	theme	biopolymeric	516:527	arg1	membrane					529:536	a biopolymeric membrane	514:536	a biopolymeric membrane	514:536	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	9	2	theme	simple	1572:1577	arg1	pathways					1593:1600	simple and promising pathways	1572:1600	simple and promising pathways	1572:1600	Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.					
35806282	2	3	from	phosphate	501:509	arg1	membrane					529:536	a biopolymeric membrane	514:536	a biopolymeric membrane	514:536	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	1	4	theme	physical	383:390	arg1	confinement					392:402	physical confinement	383:402	physical confinement during mineral phase deposition	383:434	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35806282	3	5	theme	hydrolyzed	828:837	arg1	Cht					867:869	Cht	867:869	Cht	867:869	The biopolymeric membranes were based on hydrolyzed collagen (HC) and chitosan (Cht) or κ-carrageenan (κ-carr).					
35806282	3	5	theme	hydrolyzed	828:837	arg1	HC					849:850	HC	849:850	HC	849:850	The biopolymeric membranes were based on hydrolyzed collagen (HC) and chitosan (Cht) or κ-carrageenan (κ-carr).					
35806282	3	5	theme	hydrolyzed	828:837	arg1	collagen					839:846	hydrolyzed collagen	828:846	hydrolyzed collagen (HC)	828:851	The biopolymeric membranes were based on hydrolyzed collagen (HC) and chitosan (Cht) or κ-carrageenan (κ-carr).					
35806282	3	6	theme	biopolymeric	791:802	arg1	membranes					804:812	The biopolymeric membranes	787:812	The biopolymeric membranes	787:812	The biopolymeric membranes were based on hydrolyzed collagen (HC) and chitosan (Cht) or κ-carrageenan (κ-carr).					
35806282	2	7	dep	incorporating	479:491	arg1	1					476:476	1	476:476	1	476:476	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	1	8	theme	collagen	198:205	arg1	fibrils					207:213	collagen fibrils	198:213	collagen fibrils	198:213	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35806282	5	9	from	importance	1083:1092	arg1	biomineralization					1120:1136	biomineralization	1120:1136	biomineralization	1120:1136	The importance of the confined spaces in biomineralization was confirmed by controlled biomimetic HAp precipitation via Methodology 1.					
35806282	0	10	from	Synthesis	0:8	arg1	Culture					124:130	Osteoblast Culture	113:130	Osteoblast Culture	113:130	Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture.					
35806282	2	11	theme	membrane	624:631	arg1	interstices					592:602	the interstices	588:602	the interstices of the biopolymeric membrane	588:631	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	9	12	theme	degradation	1678:1688	arg1	rates					1690:1694	controllable degradation rates	1665:1694	controllable degradation rates	1665:1694	Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.					
35806282	9	13	theme	new	1615:1617	arg1	generation					1619:1628	a new generation	1613:1628	a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties	1613:1723	Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.					
35806282	4	14	theme	mechanical	1056:1065	arg1	properties					1067:1076	mechanical properties	1056:1076	mechanical properties	1056:1076	The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties.					
35806282	2	15	theme	hydroxyapatite	550:563	arg1	precipitation					571:583	in situ hydroxyapatite (HAp) precipitation	542:583	in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane	542:631	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	1	16	dep	role	350:353	arg1	plays					374:378	plays	374:378	plays as physical confinement during mineral phase deposition	374:434	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35806282	2	17	theme	biopolymeric	611:622	arg1	membrane					624:631	the biopolymeric membrane	607:631	the biopolymeric membrane	607:631	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	4	18	theme	mineral	975:981	arg1	particles					983:991	the mineral particles	971:991	the mineral particles embedded in the biopolymeric membrane interstices	971:1041	The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties.					
35806282	1	19	theme	bioactive	305:313	arg1	membranes					322:330	bioactive hybrid membranes	305:330	bioactive hybrid membranes	305:330	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35806282	5	20	theme	confined	1101:1108	arg1	spaces					1110:1115	the confined spaces	1097:1115	the confined spaces	1097:1115	The importance of the confined spaces in biomineralization was confirmed by controlled biomimetic HAp precipitation via Methodology 1.					
35806282	6	21	theme	hybrid	1290:1295	arg1	membranes					1297:1305	the hybrid membranes	1286:1305	the hybrid membranes	1286:1305	HAp precipitation after immersion in simulated body fluid attested that the hybrid membranes were bioactive.					
35806282	8	22	theme	Hybrid	1390:1395	arg1	membranes					1397:1405	Hybrid membranes	1390:1405	Hybrid membranes added with silver nanoparticles (AgNPs)	1390:1445	Hybrid membranes added with silver nanoparticles (AgNPs) displayed antibacterial action against different clinically important pathogenic microorganisms.					
35806282	2	23	theme	in	542:543	arg1	precipitation					571:583	in situ hydroxyapatite (HAp) precipitation	542:583	in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane	542:631	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	5	24	theme	spaces	1110:1115	arg1	importance					1083:1092	The importance	1079:1092	The importance of the confined spaces in biomineralization	1079:1136	The importance of the confined spaces in biomineralization was confirmed by controlled biomimetic HAp precipitation via Methodology 1.					
35806282	4	25	theme	particles	983:991	arg1	dispersion					957:966	homogeneous and continuous dispersion	930:966	homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices	930:1041	The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties.					
35806282	2	26	theme	synthetic	689:697	arg1	SHAp					718:721	SHAp	718:721	SHAp	718:721	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	2	26	theme	synthetic	689:697	arg1	nanoparticles					703:715	synthetic HAp nanoparticles	689:715	synthetic HAp nanoparticles (SHAp)	689:722	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	1	27	dep	composition	149:159	arg1	the					145:147	the	145:147	the	145:147	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35806282	2	28	theme	biopolymeric	748:759	arg1	membrane					761:768	the freshly prepared biopolymeric membrane	727:768	the freshly prepared biopolymeric membrane (Methodology 2)	727:784	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	2	28	theme	biopolymeric	748:759	arg1	Methodology					771:781	Methodology 2	771:783	Methodology 2	771:783	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	4	29	theme	biopolymeric	1009:1020	arg1	interstices					1031:1041	the biopolymeric membrane interstices	1005:1041	the biopolymeric membrane interstices	1005:1041	The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties.					
35806282	1	30	theme	mineral	411:417	arg1	deposition					425:434	mineral phase deposition	411:434	mineral phase deposition	411:434	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35806282	4	31	from	interstices	1031:1041	arg1	embedded					993:1000	embedded	993:1000	embedded	993:1000	The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties.					
35806282	2	32	theme	prepared	739:746	arg1	membrane					761:768	the freshly prepared biopolymeric membrane	727:768	the freshly prepared biopolymeric membrane (Methodology 2)	727:784	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	2	32	theme	prepared	739:746	arg1	Methodology					771:781	Methodology 2	771:783	Methodology 2	771:783	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	1	33	theme	bone	222:225	arg1	formation					227:235	bone formation	222:235	bone formation	222:235	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35806282	1	34	theme	hybrid	315:320	arg1	membranes					322:330	bioactive hybrid membranes	305:330	bioactive hybrid membranes	305:330	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35806282	2	35	theme	confined	638:645	arg1	environment					647:657	a confined environment	636:657	a confined environment (Methodology 1)	636:673	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	2	35	theme	confined	638:645	arg1	phosphate					501:509	calcium phosphate	493:509	calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane	493:631	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	2	35	theme	confined	638:645	arg1	Methodology					660:670	Methodology 1	660:672	Methodology 1	660:672	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	9	36	theme	bioactive	1633:1641	arg1	membranes					1650:1658	bioactive hybrid membranes	1633:1658	bioactive hybrid membranes	1633:1658	Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.					
35806282	9	37	theme	promising	1583:1591	arg1	pathways					1593:1600	simple and promising pathways	1572:1600	simple and promising pathways	1572:1600	Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.					
35806282	0	38	theme	Hybrid	27:32	arg1	Membranes					83:91	Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes	13:91	Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes	13:91	Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture.					
35806282	8	39	theme	important	1507:1515	arg1	microorganisms					1528:1541	different clinically important pathogenic microorganisms	1486:1541	different clinically important pathogenic microorganisms	1486:1541	Hybrid membranes added with silver nanoparticles (AgNPs) displayed antibacterial action against different clinically important pathogenic microorganisms.					
35806282	8	40	theme	silver	1418:1423	arg1	AgNPs					1440:1444	AgNPs	1440:1444	AgNPs	1440:1444	Hybrid membranes added with silver nanoparticles (AgNPs) displayed antibacterial action against different clinically important pathogenic microorganisms.					
35806282	8	40	theme	silver	1418:1423	arg1	nanoparticles					1425:1437	silver nanoparticles	1418:1437	silver nanoparticles (AgNPs)	1418:1445	Hybrid membranes added with silver nanoparticles (AgNPs) displayed antibacterial action against different clinically important pathogenic microorganisms.					
35806282	9	41	with	generation	1619:1628	arg1	rates					1690:1694	controllable degradation rates	1665:1694	controllable degradation rates	1665:1694	Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.					
35806282	9	41	with	generation	1619:1628	arg1	properties					1714:1723	antimicrobial properties	1700:1723	antimicrobial properties	1700:1723	Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.					
35806282	0	42	theme	Antibacterial	13:25	arg1	Membranes					83:91	Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes	13:91	Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes	13:91	Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture.					
35806282	2	43	dep	in	542:543	arg1	situ					545:548	situ	545:548	situ	545:548	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	1	44	theme	phase	419:423	arg1	deposition					425:434	mineral phase deposition	411:434	mineral phase deposition	411:434	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35806282	9	45	theme	membranes	1650:1658	arg1	generation					1619:1628	a new generation	1613:1628	a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties	1613:1723	Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.					
35806282	0	46	theme	Bioactive	73:81	arg1	Membranes					83:91	Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes	13:91	Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes	13:91	Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture.					
35806282	4	47	theme	hybrid	903:908	arg1	membranes					910:918	The hybrid membranes	899:918	The hybrid membranes	899:918	The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties.					
35806282	8	48	theme	different	1486:1494	arg1	microorganisms					1528:1541	different clinically important pathogenic microorganisms	1486:1541	different clinically important pathogenic microorganisms	1486:1541	Hybrid membranes added with silver nanoparticles (AgNPs) displayed antibacterial action against different clinically important pathogenic microorganisms.					
35806282	9	49	theme	controllable	1665:1676	arg1	rates					1690:1694	controllable degradation rates	1665:1694	controllable degradation rates	1665:1694	Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.					
35806282	7	50	theme	Hybrid	1323:1328	arg1	membranes					1330:1338	Hybrid membranes	1323:1338	Hybrid membranes containing Cht	1323:1353	Hybrid membranes containing Cht were not toxic to the osteoblasts.					
35806282	0	51	theme	Hydroxyapatite/Collagen/Polysaccharide	34:71	arg1	Membranes					83:91	Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes	13:91	Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes	13:91	Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture.					
35806282	4	52	theme	embedded	993:1000	arg1	particles					983:991	the mineral particles	971:991	the mineral particles embedded in the biopolymeric membrane interstices	971:1041	The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties.					
35806282	2	53	theme	HAp	566:568	arg1	precipitation					571:583	in situ hydroxyapatite (HAp) precipitation	542:583	in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane	542:631	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	0	54	from	Effect	103:108	arg1	Culture					124:130	Osteoblast Culture	113:130	Osteoblast Culture	113:130	Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture.					
35806282	6	55	theme	body	1261:1264	arg1	fluid					1266:1270	simulated body fluid	1251:1270	simulated body fluid attested that the hybrid membranes	1251:1305	HAp precipitation after immersion in simulated body fluid attested that the hybrid membranes were bioactive.					
35806282	2	56	theme	hybrid	441:446	arg1	membranes					448:456	The hybrid membranes	437:456	The hybrid membranes	437:456	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	2	57	theme	calcium	493:499	arg1	environment					647:657	a confined environment	636:657	a confined environment (Methodology 1)	636:673	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	2	57	theme	calcium	493:499	arg1	phosphate					501:509	calcium phosphate	493:509	calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane	493:631	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	4	58	from	embedded	993:1000	arg1	interstices					1031:1041	the biopolymeric membrane interstices	1005:1041	the biopolymeric membrane interstices	1005:1041	The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties.					
35806282	7	59	contain	containing	1340:1349	arg2	Cht					1351:1353	Cht	1351:1353	Cht	1351:1353	Hybrid membranes containing Cht were not toxic to the osteoblasts.					
35806282	7	59	contain	containing	1340:1349	arg1	membranes					1330:1338	Hybrid membranes	1323:1338	Hybrid membranes containing Cht	1323:1353	Hybrid membranes containing Cht were not toxic to the osteoblasts.					
35806282	6	60	theme	simulated	1251:1259	arg1	fluid					1266:1270	simulated body fluid	1251:1270	simulated body fluid attested that the hybrid membranes	1251:1305	HAp precipitation after immersion in simulated body fluid attested that the hybrid membranes were bioactive.					
35806282	0	61	theme	Membranes	83:91	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes	0:91	Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture.					
35806282	0	61	theme	Membranes	83:91	arg1	Effect					103:108	Their Effect	97:108	Their Effect on Osteoblast Culture	97:130	Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture.					
35806282	5	62	theme	HAp	1177:1179	arg1	precipitation					1181:1193	controlled biomimetic HAp precipitation	1155:1193	controlled biomimetic HAp precipitation via Methodology 1	1155:1211	The importance of the confined spaces in biomineralization was confirmed by controlled biomimetic HAp precipitation via Methodology 1.					
35806282	5	63	theme	controlled	1155:1164	arg1	precipitation					1181:1193	controlled biomimetic HAp precipitation	1155:1193	controlled biomimetic HAp precipitation via Methodology 1	1155:1211	The importance of the confined spaces in biomineralization was confirmed by controlled biomimetic HAp precipitation via Methodology 1.					
35806282	8	64	theme	pathogenic	1517:1526	arg1	microorganisms					1528:1541	different clinically important pathogenic microorganisms	1486:1541	different clinically important pathogenic microorganisms	1486:1541	Hybrid membranes added with silver nanoparticles (AgNPs) displayed antibacterial action against different clinically important pathogenic microorganisms.					
35806282	6	65	theme	HAp	1214:1216	arg1	precipitation					1218:1230	HAp precipitation	1214:1230	HAp precipitation after immersion in simulated body fluid attested that the hybrid membranes	1214:1305	HAp precipitation after immersion in simulated body fluid attested that the hybrid membranes were bioactive.					
35806282	4	66	theme	membrane	1022:1029	arg1	interstices					1031:1041	the biopolymeric membrane interstices	1005:1041	the biopolymeric membrane interstices	1005:1041	The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties.					
35806282	8	67	theme	antibacterial	1457:1469	arg1	action					1471:1476	antibacterial action	1457:1476	antibacterial action against different clinically important pathogenic microorganisms	1457:1541	Hybrid membranes added with silver nanoparticles (AgNPs) displayed antibacterial action against different clinically important pathogenic microorganisms.					
35806282	2	68	theme	HAp	699:701	arg1	SHAp					718:721	SHAp	718:721	SHAp	718:721	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	2	68	theme	HAp	699:701	arg1	nanoparticles					703:715	synthetic HAp nanoparticles	689:715	synthetic HAp nanoparticles (SHAp)	689:722	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	9	69	theme	hybrid	1643:1648	arg1	membranes					1650:1658	bioactive hybrid membranes	1633:1658	bioactive hybrid membranes	1633:1658	Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.					
35806282	1	70	dep	strategies	280:289	arg1	synthesize					294:303	synthesize	294:303	to synthesize bioactive hybrid membranes	291:330	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35806282	9	71	theme	antimicrobial	1700:1712	arg1	properties					1714:1723	antimicrobial properties	1700:1723	antimicrobial properties	1700:1723	Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.					
35806282	1	72	theme	confined	165:172	arg1	environment					174:184	confined environment	165:184	confined environment	165:184	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35806282	5	73	theme	biomimetic	1166:1175	arg1	precipitation					1181:1193	controlled biomimetic HAp precipitation	1155:1193	controlled biomimetic HAp precipitation via Methodology 1	1155:1211	The importance of the confined spaces in biomineralization was confirmed by controlled biomimetic HAp precipitation via Methodology 1.					
35806282	2	74	dep	adding	682:687	arg1	2					679:679	2	679:679	2	679:679	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	4	75	theme	continuous	946:955	arg1	dispersion					957:966	homogeneous and continuous dispersion	930:966	homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices	930:1041	The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties.					
35806282	1	76	theme	organic	359:365	arg1	matrix					367:372	the organic matrix	355:372	the organic matrix	355:372	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35806282	2	77	from	precipitation	571:583	arg1	interstices					592:602	the interstices	588:602	the interstices of the biopolymeric membrane	588:631	The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2).					
35806282	4	78	theme	homogeneous	930:940	arg1	dispersion					957:966	homogeneous and continuous dispersion	930:966	homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices	930:1041	The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties.					
35806282	6	79	from	precipitation	1218:1230	arg1	fluid					1266:1270	simulated body fluid	1251:1270	simulated body fluid attested that the hybrid membranes	1251:1305	HAp precipitation after immersion in simulated body fluid attested that the hybrid membranes were bioactive.					
35806282	1	80	theme	different	270:278	arg1	strategies					280:289	two different strategies	266:289	two different strategies to synthesize bioactive hybrid membranes	266:330	Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition.					
35871176	11	0	theme	body	1952:1955	arg1	weight					1957:1962	improved body weight	1943:1962	improved body weight	1943:1962	Future research into the effects of specific metabolites identified in this study will provide deeper insight into the mechanism of fiber supplementation on improved body weight.					
35871176	9	1	theme	bile	1456:1459	arg1	acids					1461:1465	lower total bile acids	1444:1465	lower total bile acids	1444:1465	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	6	2	theme	bile	942:945	arg1	acids					947:951	enterohepatic bile acids	928:951	enterohepatic bile acids	928:951	Quantification of enterohepatic bile acids was performed with UPLC-MS to determine specific effects of obesity and fiber supplementation on the bile acid pool composition.					
35871176	9	3	from	supplementation	1582:1596	arg1	fat-feeding					1606:1616	high fat-feeding	1601:1616	high fat-feeding	1601:1616	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	4	4	theme	healthy	621:627	arg1	rats					639:642	healthy and obese rats	621:642	healthy and obese rats	621:642	OBJECTIVES To investigate and compare the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS).					
35871176	7	5	theme	healthy	1154:1160	arg1	rats					1162:1165	healthy rats	1154:1165	healthy rats	1154:1165	RESULTS The small intestinal metabolome of obese rats was distinct from healthy rats.					
35871176	2	6	theme	Prebiotic	291:299	arg1	supplementation					301:315	Prebiotic supplementation	291:315	Prebiotic supplementation	291:315	Prebiotic supplementation can reverse obesity and beneficially alter the gut microbiome, evidenced by previous studies in rodents.					
35871176	1	7	theme	gut	275:277	arg1	microbiome					279:288	an altered gut microbiome	264:288	an altered gut microbiome	264:288	INTRODUCTION Obesity occurs partly due to consumption of a high-fat, high-sugar and low fiber diet and is associated with an altered gut microbiome.					
35871176	8	8	theme	small	1220:1224	arg1	metabolome					1237:1246	the small intestinal metabolome	1216:1246	the small intestinal metabolome	1216:1246	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	10	9	theme	CONCLUSION	1619:1628	arg1	Obesity					1630:1636	CONCLUSION Obesity	1619:1636	CONCLUSION Obesity	1619:1636	CONCLUSION Obesity is associated with a distinct small intestinal metabolome, and OFS supplementation reverses some metabolite levels that were altered in obese rats.					
35871176	9	10	theme	taurine-conjugated	1481:1498	arg1	species					1510:1516	increased taurine-conjugated bile acid species	1471:1516	increased taurine-conjugated bile acid species	1471:1516	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	8	11	theme	obese	1304:1308	arg1	rats					1310:1313	obese rats	1304:1313	obese rats	1304:1313	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	10	12	theme	distinct	1659:1666	arg1	metabolome					1685:1694	a distinct small intestinal metabolome	1657:1694	a distinct small intestinal metabolome	1657:1694	CONCLUSION Obesity is associated with a distinct small intestinal metabolome, and OFS supplementation reverses some metabolite levels that were altered in obese rats.					
35871176	7	13	theme	rats	1131:1134	arg1	metabolome					1111:1120	The small intestinal metabolome	1090:1120	The small intestinal metabolome of obese rats	1090:1134	RESULTS The small intestinal metabolome of obese rats was distinct from healthy rats.					
35871176	9	14	theme	acid	1505:1508	arg1	species					1510:1516	increased taurine-conjugated bile acid species	1471:1516	increased taurine-conjugated bile acid species	1471:1516	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	5	15	theme	obese	849:853	arg1	rats					855:858	obese rats	849:858	obese rats supplemented with oligofructose using UPLC-MS/MS	849:907	METHODS Untargeted metabolomics was performed on small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS.					
35871176	6	16	theme	specific	993:1000	arg1	effects					1002:1008	specific effects	993:1008	specific effects of obesity and fiber supplementation on the bile acid pool composition	993:1079	Quantification of enterohepatic bile acids was performed with UPLC-MS to determine specific effects of obesity and fiber supplementation on the bile acid pool composition.					
35871176	2	17	theme	previous	393:400	arg1	studies					402:408	previous studies	393:408	previous studies in rodents	393:419	Prebiotic supplementation can reverse obesity and beneficially alter the gut microbiome, evidenced by previous studies in rodents.					
35871176	4	18	theme	obese	656:660	arg1	rats					662:665	obese rats	656:665	obese rats supplemented with the prebiotic oligofructose (OFS)	656:717	OBJECTIVES To investigate and compare the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS).					
35871176	7	19	theme	intestinal	1100:1109	arg1	metabolome					1111:1120	The small intestinal metabolome	1090:1120	The small intestinal metabolome of obese rats	1090:1134	RESULTS The small intestinal metabolome of obese rats was distinct from healthy rats.					
35871176	1	20	theme	fiber	230:234	arg1	diet					236:239	a high-fat, high-sugar and low fiber diet	199:239	a high-fat, high-sugar and low fiber diet	199:239	INTRODUCTION Obesity occurs partly due to consumption of a high-fat, high-sugar and low fiber diet and is associated with an altered gut microbiome.					
35871176	8	21	theme	several	1272:1278	arg1	metabolites					1360:1370	amino acid metabolites	1349:1370	amino acid metabolites	1349:1370	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	8	21	theme	several	1272:1278	arg1	metabolites					1280:1290	several metabolites	1272:1290	several metabolites	1272:1290	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	8	21	theme	several	1272:1278	arg1	metabolites related					1377:1395	metabolites related	1377:1395	metabolites related	1377:1395	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	8	21	theme	several	1272:1278	arg1	metabolites					1336:1346	bile acid metabolites	1326:1346	bile acid metabolites	1326:1346	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	10	22	theme	intestinal	1674:1683	arg1	metabolome					1685:1694	a distinct small intestinal metabolome	1657:1694	a distinct small intestinal metabolome	1657:1694	CONCLUSION Obesity is associated with a distinct small intestinal metabolome, and OFS supplementation reverses some metabolite levels that were altered in obese rats.					
35871176	1	23	theme	INTRODUCTION	142:153	arg1	Obesity					155:161	INTRODUCTION Obesity	142:161	INTRODUCTION Obesity	142:161	INTRODUCTION Obesity occurs partly due to consumption of a high-fat, high-sugar and low fiber diet and is associated with an altered gut microbiome.					
35871176	8	24	theme	amino	1349:1353	arg1	metabolites					1360:1370	amino acid metabolites	1349:1370	amino acid metabolites	1349:1370	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	6	25	theme	acid	1059:1062	arg1	composition					1069:1079	the bile acid pool composition	1050:1079	the bile acid pool composition	1050:1079	Quantification of enterohepatic bile acids was performed with UPLC-MS to determine specific effects of obesity and fiber supplementation on the bile acid pool composition.					
35871176	5	26	dep	METHODS	720:726	arg1	metabolomics					739:750	Untargeted metabolomics	728:750	METHODS Untargeted metabolomics	720:750	METHODS Untargeted metabolomics was performed on small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS.					
35871176	5	27	theme	intestinal	775:784	arg1	contents					786:793	small intestinal contents	769:793	small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS	769:907	METHODS Untargeted metabolomics was performed on small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS.					
35871176	0	28	theme	obese	95:99	arg1	rats					101:104	obese rats	95:104	obese rats	95:104	Small intestinal metabolomics analysis reveals differentially regulated metabolite profiles in obese rats and with prebiotic supplementation.					
35871176	6	29	theme	supplementation	1031:1045	arg1	effects					1002:1008	specific effects	993:1008	specific effects of obesity and fiber supplementation on the bile acid pool composition	993:1079	Quantification of enterohepatic bile acids was performed with UPLC-MS to determine specific effects of obesity and fiber supplementation on the bile acid pool composition.					
35871176	11	30	theme	metabolites	1831:1841	arg1	effects					1811:1817	the effects	1807:1817	the effects of specific metabolites identified in this study	1807:1866	Future research into the effects of specific metabolites identified in this study will provide deeper insight into the mechanism of fiber supplementation on improved body weight.					
35871176	9	31	theme	high	1601:1604	arg1	fat-feeding					1606:1616	high fat-feeding	1601:1616	high fat-feeding	1601:1616	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	8	32	theme	gut	1404:1406	arg1	microbiota					1408:1417	the gut microbiota	1400:1417	the gut microbiota	1400:1417	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	0	33	theme	prebiotic	115:123	arg1	supplementation					125:139	prebiotic supplementation	115:139	prebiotic supplementation	115:139	Small intestinal metabolomics analysis reveals differentially regulated metabolite profiles in obese rats and with prebiotic supplementation.					
35871176	6	34	from	effects	1002:1008	arg1	composition					1069:1079	the bile acid pool composition	1050:1079	the bile acid pool composition	1050:1079	Quantification of enterohepatic bile acids was performed with UPLC-MS to determine specific effects of obesity and fiber supplementation on the bile acid pool composition.					
35871176	0	35	theme	metabolomics	17:28	arg1	analysis					30:37	Small intestinal metabolomics analysis	0:37	Small intestinal metabolomics analysis	0:37	Small intestinal metabolomics analysis reveals differentially regulated metabolite profiles in obese rats and with prebiotic supplementation.					
35871176	3	36	theme	intestinal	453:462	arg1	metabolome					464:473	the small intestinal metabolome	443:473	the small intestinal metabolome	443:473	However, the role of the small intestinal metabolome in obese and prebiotic supplemented rodents has never been investigated.					
35871176	5	37	theme	chow-fed	806:813	arg1	contents					786:793	small intestinal contents	769:793	small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS	769:907	METHODS Untargeted metabolomics was performed on small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS.					
35871176	2	38	from	studies	402:408	arg1	rodents					413:419	rodents	413:419	rodents	413:419	Prebiotic supplementation can reverse obesity and beneficially alter the gut microbiome, evidenced by previous studies in rodents.					
35871176	2	39	theme	gut	364:366	arg1	microbiome					368:377	the gut microbiome	360:377	the gut microbiome	360:377	Prebiotic supplementation can reverse obesity and beneficially alter the gut microbiome, evidenced by previous studies in rodents.					
35871176	0	40	theme	regulated	62:70	arg1	profiles					83:90	differentially regulated metabolite profiles	47:90	differentially regulated metabolite profiles	47:90	Small intestinal metabolomics analysis reveals differentially regulated metabolite profiles in obese rats and with prebiotic supplementation.					
35871176	9	41	theme	obese	1429:1433	arg1	rats					1435:1438	obese rats	1429:1438	obese rats	1429:1438	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	4	42	theme	prebiotic	689:697	arg1	OFS					714:716	OFS	714:716	OFS	714:716	OBJECTIVES To investigate and compare the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS).					
35871176	4	42	theme	prebiotic	689:697	arg1	oligofructose					699:711	the prebiotic oligofructose	685:711	the prebiotic oligofructose (OFS)	685:717	OBJECTIVES To investigate and compare the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS).					
35871176	3	43	theme	metabolome	464:473	arg1	role					435:438	the role	431:438	the role of the small intestinal metabolome in obese and prebiotic supplemented rodents	431:517	However, the role of the small intestinal metabolome in obese and prebiotic supplemented rodents has never been investigated.					
35871176	8	44	theme	metabolites	1280:1290	arg1	levels					1262:1267	levels	1262:1267	levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota	1262:1417	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	8	45	theme	OFS	1168:1170	arg1	supplementation					1172:1186	OFS supplementation	1168:1186	OFS supplementation	1168:1186	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	4	46	theme	rats	639:642	arg1	metabolome					607:616	the small intestinal metabolome	586:616	the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS)	586:717	OBJECTIVES To investigate and compare the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS).					
35871176	11	47	theme	improved	1943:1950	arg1	weight					1957:1962	improved body weight	1943:1962	improved body weight	1943:1962	Future research into the effects of specific metabolites identified in this study will provide deeper insight into the mechanism of fiber supplementation on improved body weight.					
35871176	9	48	theme	total	1450:1454	arg1	acids					1461:1465	lower total bile acids	1444:1465	lower total bile acids	1444:1465	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	6	49	theme	acids	947:951	arg1	Quantification					910:923	Quantification	910:923	Quantification of enterohepatic bile acids	910:951	Quantification of enterohepatic bile acids was performed with UPLC-MS to determine specific effects of obesity and fiber supplementation on the bile acid pool composition.					
35871176	4	50	theme	intestinal	596:605	arg1	metabolome					607:616	the small intestinal metabolome	586:616	the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS)	586:717	OBJECTIVES To investigate and compare the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS).					
35871176	9	51	contain	had	1440:1442	arg2	acids					1461:1465	lower total bile acids	1444:1465	lower total bile acids	1444:1465	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	9	51	contain	had	1440:1442	arg1	rats					1435:1438	obese rats	1429:1438	obese rats	1429:1438	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	9	51	contain	had	1440:1442	arg2	species					1510:1516	increased taurine-conjugated bile acid species	1471:1516	increased taurine-conjugated bile acid species	1471:1516	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	1	52	theme	altered	267:273	arg1	microbiome					279:288	an altered gut microbiome	264:288	an altered gut microbiome	264:288	INTRODUCTION Obesity occurs partly due to consumption of a high-fat, high-sugar and low fiber diet and is associated with an altered gut microbiome.					
35871176	6	53	theme	enterohepatic	928:940	arg1	acids					947:951	enterohepatic bile acids	928:951	enterohepatic bile acids	928:951	Quantification of enterohepatic bile acids was performed with UPLC-MS to determine specific effects of obesity and fiber supplementation on the bile acid pool composition.					
35871176	7	54	dep	RESULTS	1082:1088	arg1	distinct					1140:1147	distinct	1140:1147	distinct	1140:1147	RESULTS The small intestinal metabolome of obese rats was distinct from healthy rats.					
35871176	3	55	theme	supplemented	498:509	arg1	rodents					511:517	obese and prebiotic supplemented rodents	478:517	obese and prebiotic supplemented rodents	478:517	However, the role of the small intestinal metabolome in obese and prebiotic supplemented rodents has never been investigated.					
35871176	1	56	theme	high-fat	201:208	arg1	diet					236:239	a high-fat, high-sugar and low fiber diet	199:239	a high-fat, high-sugar and low fiber diet	199:239	INTRODUCTION Obesity occurs partly due to consumption of a high-fat, high-sugar and low fiber diet and is associated with an altered gut microbiome.					
35871176	9	57	theme	increased	1471:1479	arg1	species					1510:1516	increased taurine-conjugated bile acid species	1471:1516	increased taurine-conjugated bile acid species	1471:1516	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	4	58	theme	rats	662:665	arg1	metabolome					607:616	the small intestinal metabolome	586:616	the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS)	586:717	OBJECTIVES To investigate and compare the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS).					
35871176	10	59	theme	small	1668:1672	arg1	metabolome					1685:1694	a distinct small intestinal metabolome	1657:1694	a distinct small intestinal metabolome	1657:1694	CONCLUSION Obesity is associated with a distinct small intestinal metabolome, and OFS supplementation reverses some metabolite levels that were altered in obese rats.					
35871176	7	60	theme	obese	1125:1129	arg1	rats					1131:1134	obese rats	1125:1134	obese rats	1125:1134	RESULTS The small intestinal metabolome of obese rats was distinct from healthy rats.					
35871176	9	61	theme	bile	1500:1503	arg1	species					1510:1516	increased taurine-conjugated bile acid species	1471:1516	increased taurine-conjugated bile acid species	1471:1516	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	8	62	theme	intestinal	1226:1235	arg1	metabolome					1237:1246	the small intestinal metabolome	1216:1246	the small intestinal metabolome	1216:1246	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	6	63	theme	obesity	1013:1019	arg1	effects					1002:1008	specific effects	993:1008	specific effects of obesity and fiber supplementation on the bile acid pool composition	993:1079	Quantification of enterohepatic bile acids was performed with UPLC-MS to determine specific effects of obesity and fiber supplementation on the bile acid pool composition.					
35871176	1	64	theme	diet	236:239	arg1	consumption					184:194	consumption	184:194	consumption of a high-fat, high-sugar and low fiber diet	184:239	INTRODUCTION Obesity occurs partly due to consumption of a high-fat, high-sugar and low fiber diet and is associated with an altered gut microbiome.					
35871176	1	65	theme	high-sugar	211:220	arg1	diet					236:239	a high-fat, high-sugar and low fiber diet	199:239	a high-fat, high-sugar and low fiber diet	199:239	INTRODUCTION Obesity occurs partly due to consumption of a high-fat, high-sugar and low fiber diet and is associated with an altered gut microbiome.					
35871176	7	66	theme	small	1094:1098	arg1	metabolome					1111:1120	The small intestinal metabolome	1090:1120	The small intestinal metabolome of obese rats	1090:1134	RESULTS The small intestinal metabolome of obese rats was distinct from healthy rats.					
35871176	5	67	theme	rats	855:858	arg1	contents					786:793	small intestinal contents	769:793	small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS	769:907	METHODS Untargeted metabolomics was performed on small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS.					
35871176	8	68	theme	bile	1326:1329	arg1	metabolites					1336:1346	bile acid metabolites	1326:1346	bile acid metabolites	1326:1346	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	11	69	theme	deeper	1881:1886	arg1	insight					1888:1894	deeper insight	1881:1894	deeper insight into the mechanism of fiber supplementation	1881:1938	Future research into the effects of specific metabolites identified in this study will provide deeper insight into the mechanism of fiber supplementation on improved body weight.					
35871176	8	70	theme	acid	1331:1334	arg1	metabolites					1336:1346	bile acid metabolites	1326:1346	bile acid metabolites	1326:1346	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	6	71	theme	pool	1064:1067	arg1	composition					1069:1079	the bile acid pool composition	1050:1079	the bile acid pool composition	1050:1079	Quantification of enterohepatic bile acids was performed with UPLC-MS to determine specific effects of obesity and fiber supplementation on the bile acid pool composition.					
35871176	11	72	theme	specific	1822:1829	arg1	metabolites					1831:1841	specific metabolites	1822:1841	specific metabolites identified in this study	1822:1866	Future research into the effects of specific metabolites identified in this study will provide deeper insight into the mechanism of fiber supplementation on improved body weight.					
35871176	5	73	theme	small	769:773	arg1	contents					786:793	small intestinal contents	769:793	small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS	769:907	METHODS Untargeted metabolomics was performed on small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS.					
35871176	6	74	theme	bile	1054:1057	arg1	composition					1069:1079	the bile acid pool composition	1050:1079	the bile acid pool composition	1050:1079	Quantification of enterohepatic bile acids was performed with UPLC-MS to determine specific effects of obesity and fiber supplementation on the bile acid pool composition.					
35871176	8	75	theme	acid	1355:1358	arg1	metabolites					1360:1370	amino acid metabolites	1349:1370	amino acid metabolites	1349:1370	OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and metabolites related to the gut microbiota.					
35871176	9	76	theme	OFS	1578:1580	arg1	supplementation					1582:1596	OFS supplementation	1578:1596	OFS supplementation in high fat-feeding	1578:1616	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	11	77	theme	supplementation	1924:1938	arg1	mechanism					1905:1913	the mechanism	1901:1913	the mechanism of fiber supplementation	1901:1938	Future research into the effects of specific metabolites identified in this study will provide deeper insight into the mechanism of fiber supplementation on improved body weight.					
35871176	6	78	theme	fiber	1025:1029	arg1	supplementation					1031:1045	fiber supplementation	1025:1045	fiber supplementation	1025:1045	Quantification of enterohepatic bile acids was performed with UPLC-MS to determine specific effects of obesity and fiber supplementation on the bile acid pool composition.					
35871176	11	79	theme	fiber	1918:1922	arg1	supplementation					1924:1938	fiber supplementation	1918:1938	fiber supplementation	1918:1938	Future research into the effects of specific metabolites identified in this study will provide deeper insight into the mechanism of fiber supplementation on improved body weight.					
35871176	3	80	theme	small	447:451	arg1	metabolome					464:473	the small intestinal metabolome	443:473	the small intestinal metabolome	443:473	However, the role of the small intestinal metabolome in obese and prebiotic supplemented rodents has never been investigated.					
35871176	0	81	theme	intestinal	6:15	arg1	analysis					30:37	Small intestinal metabolomics analysis	0:37	Small intestinal metabolomics analysis	0:37	Small intestinal metabolomics analysis reveals differentially regulated metabolite profiles in obese rats and with prebiotic supplementation.					
35871176	11	82	theme	Future	1786:1791	arg1	research					1793:1800	Future research	1786:1800	Future research into the effects of specific metabolites identified in this study	1786:1866	Future research into the effects of specific metabolites identified in this study will provide deeper insight into the mechanism of fiber supplementation on improved body weight.					
35871176	10	83	theme	OFS	1701:1703	arg1	supplementation					1705:1719	OFS supplementation	1701:1719	OFS supplementation	1701:1719	CONCLUSION Obesity is associated with a distinct small intestinal metabolome, and OFS supplementation reverses some metabolite levels that were altered in obese rats.					
35871176	5	84	theme	obese	838:842	arg1	contents					786:793	small intestinal contents	769:793	small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS	769:907	METHODS Untargeted metabolomics was performed on small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS.					
35871176	4	85	theme	small	590:594	arg1	metabolome					607:616	the small intestinal metabolome	586:616	the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS)	586:717	OBJECTIVES To investigate and compare the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS).					
35871176	3	86	theme	obese	478:482	arg1	rodents					511:517	obese and prebiotic supplemented rodents	478:517	obese and prebiotic supplemented rodents	478:517	However, the role of the small intestinal metabolome in obese and prebiotic supplemented rodents has never been investigated.					
35871176	1	87	theme	low	226:228	arg1	diet					236:239	a high-fat, high-sugar and low fiber diet	199:239	a high-fat, high-sugar and low fiber diet	199:239	INTRODUCTION Obesity occurs partly due to consumption of a high-fat, high-sugar and low fiber diet and is associated with an altered gut microbiome.					
35871176	10	88	theme	metabolite	1735:1744	arg1	levels					1746:1751	some metabolite levels	1730:1751	some metabolite levels that were altered in obese rats	1730:1783	CONCLUSION Obesity is associated with a distinct small intestinal metabolome, and OFS supplementation reverses some metabolite levels that were altered in obese rats.					
35871176	0	89	theme	metabolite	72:81	arg1	profiles					83:90	differentially regulated metabolite profiles	47:90	differentially regulated metabolite profiles	47:90	Small intestinal metabolomics analysis reveals differentially regulated metabolite profiles in obese rats and with prebiotic supplementation.					
35871176	9	90	theme	enterohepatic	1521:1533	arg1	circulation					1535:1545	enterohepatic circulation	1521:1545	enterohepatic circulation	1521:1545	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
35871176	5	91	theme	Untargeted	728:737	arg1	metabolomics					739:750	Untargeted metabolomics	728:750	METHODS Untargeted metabolomics	720:750	METHODS Untargeted metabolomics was performed on small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS.					
35871176	7	92	from	rats	1162:1165	arg1	distinct					1140:1147	distinct	1140:1147	distinct	1140:1147	RESULTS The small intestinal metabolome of obese rats was distinct from healthy rats.					
35871176	10	93	theme	obese	1774:1778	arg1	rats					1780:1783	obese rats	1774:1783	obese rats	1774:1783	CONCLUSION Obesity is associated with a distinct small intestinal metabolome, and OFS supplementation reverses some metabolite levels that were altered in obese rats.					
35871176	4	94	theme	obese	633:637	arg1	rats					639:642	healthy and obese rats	621:642	healthy and obese rats	621:642	OBJECTIVES To investigate and compare the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS).					
35871176	3	95	from	role	435:438	arg1	rodents					511:517	obese and prebiotic supplemented rodents	478:517	obese and prebiotic supplemented rodents	478:517	However, the role of the small intestinal metabolome in obese and prebiotic supplemented rodents has never been investigated.					
35871176	3	96	theme	prebiotic	488:496	arg1	rodents					511:517	obese and prebiotic supplemented rodents	478:517	obese and prebiotic supplemented rodents	478:517	However, the role of the small intestinal metabolome in obese and prebiotic supplemented rodents has never been investigated.					
35871176	9	97	theme	lower	1444:1448	arg1	acids					1461:1465	lower total bile acids	1444:1465	lower total bile acids	1444:1465	Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding.					
37023879	4	0	dep	structure	533:541	arg1	the					516:518	the	516:518	the	516:518	Additionally the coordination structure and morphology of Bridge-CS-CT-Schiff base/OH-CCa were verified.					
37023879	5	1	theme	size	666:669	arg1	μm					682:683	the size: 3.55~9.33 μm	662:683	the size: 3.55~9.33 μm	662:683	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	3	2	theme	high-pressure	473:485	arg1	homogenization					487:500	microwave-assisted high-pressure homogenization	454:500	microwave-assisted high-pressure homogenization	454:500	This work attempted to prepare CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa) as raw material by microwave-assisted high-pressure homogenization.					
37023879	5	3	theme	sustained	742:750	arg1	ability					761:767	an excellent sustained released ability	729:767	an excellent sustained released ability (sustained release up to 180 min)	729:801	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	5	4	theme	sustained	770:778	arg1	release					780:786	sustained release	770:786	sustained release up to 180 min	770:800	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	6	5	dep	strong	945:950	arg1	MIC					953:955	MIC	953:955	MIC of 128 μg/ml	953:968	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	5	6	theme	prepared	612:619	arg1	CS-CT-CCa					621:629	The prepared CS-CT-CCa	608:629	The prepared CS-CT-CCa	608:629	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	0	7	theme	citrate	102:108	arg1	release					120:126	a calcium citrate sustained release	92:126	a calcium citrate sustained release antibacterial agent	92:146	Characterization and antivibrio activity of chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent.					
37023879	5	8	dep	min	798:800	arg1	up					788:789	up	788:789	up	788:789	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	6	9	contain	had	941:943	arg2	effects					1013:1019	strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects	945:1019	strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects	945:1019	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	6	9	contain	had	941:943	arg1	CS-CT-CCa					931:939	CS-CT-CCa	931:939	CS-CT-CCa	931:939	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	9	10	theme	further	1405:1411	arg1	design					1413:1418	further design	1405:1418	further design	1405:1418	This study provided necessary data for the further design and development of chitosan antibacterial agents, food and feed additives.					
37023879	0	11	theme	calcium	94:100	arg1	release					120:126	a calcium citrate sustained release	92:126	a calcium citrate sustained release antibacterial agent	92:146	Characterization and antivibrio activity of chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent.					
37023879	8	12	theme	formation	1317:1325	arg1	inhibition					1295:1304	inhibition	1295:1304	inhibition of biofilm formation, swimming and swarming motilities	1295:1359	It could be inferred that the antibacterial activities against V. parahaemolyticus caused inhibition of biofilm formation, swimming and swarming motilities.					
37023879	6	13	theme	V.	1029:1030	arg1	parahaemolyticus					1032:1047	V. parahaemolyticus	1029:1047	V. parahaemolyticus	1029:1047	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	8	14	theme	V.	1268:1269	arg1	parahaemolyticus					1271:1286	V. parahaemolyticus	1268:1286	V. parahaemolyticus	1268:1286	It could be inferred that the antibacterial activities against V. parahaemolyticus caused inhibition of biofilm formation, swimming and swarming motilities.					
37023879	0	15	theme	release	120:126	arg1	agent					142:146	a calcium citrate sustained release antibacterial agent	92:146	a calcium citrate sustained release antibacterial agent	92:146	Characterization and antivibrio activity of chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent.					
37023879	5	16	theme	well-dispersed	637:650	arg1	property					652:659	a well-dispersed property	635:659	a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV)	635:723	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	9	17	theme	agents	1462:1467	arg1	development					1424:1434	development	1424:1434	development	1424:1434	This study provided necessary data for the further design and development of chitosan antibacterial agents, food and feed additives.					
37023879	9	17	theme	agents	1462:1467	arg1	design					1413:1418	further design	1405:1418	further design	1405:1418	This study provided necessary data for the further design and development of chitosan antibacterial agents, food and feed additives.					
37023879	1	18	theme	Number	180:185	arg1	"					197:197	the "Number one killer"	175:197	the "Number one killer" of seafood products	175:217	Vibrio parahemolyticus is the "Number one killer" of seafood products.					
37023879	1	18	theme	Number	180:185	arg1	parahemolyticus					156:170	Vibrio parahemolyticus	149:170	Vibrio parahemolyticus	149:170	Vibrio parahemolyticus is the "Number one killer" of seafood products.					
37023879	0	19	theme	sustained	110:118	arg1	release					120:126	a calcium citrate sustained release	92:126	a calcium citrate sustained release antibacterial agent	92:146	Characterization and antivibrio activity of chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent.					
37023879	6	20	theme	inhibition	853:862	arg1	assay					864:868	biofilm formation inhibition assay	835:868	biofilm formation inhibition assay	835:868	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	6	20	theme	inhibition	853:862	arg1	MIC					804:806	MIC	804:806	MIC	804:806	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	7	21	theme	membrane	1090:1097	arg1	permeability					1099:1110	the membrane permeability	1086:1110	the membrane permeability of V. parahaemolyticus	1086:1133	Meanwhile, CS-CT-CCa could increase the membrane permeability of V. parahaemolyticus and inhibit their biofilm-forming ability in a dose-dependent manner.					
37023879	8	22	theme	swimming	1328:1335	arg1	inhibition					1295:1304	inhibition	1295:1304	inhibition of biofilm formation, swimming and swarming motilities	1295:1359	It could be inferred that the antibacterial activities against V. parahaemolyticus caused inhibition of biofilm formation, swimming and swarming motilities.					
37023879	1	23	theme	one	187:189	arg1	"					197:197	the "Number one killer"	175:197	the "Number one killer" of seafood products	175:217	Vibrio parahemolyticus is the "Number one killer" of seafood products.					
37023879	1	23	theme	one	187:189	arg1	parahemolyticus					156:170	Vibrio parahemolyticus	149:170	Vibrio parahemolyticus	149:170	Vibrio parahemolyticus is the "Number one killer" of seafood products.					
37023879	7	24	theme	parahaemolyticus	1118:1133	arg1	permeability					1099:1110	the membrane permeability	1086:1110	the membrane permeability of V. parahaemolyticus	1086:1133	Meanwhile, CS-CT-CCa could increase the membrane permeability of V. parahaemolyticus and inhibit their biofilm-forming ability in a dose-dependent manner.					
37023879	6	25	theme	formation	843:851	arg1	assay					864:868	biofilm formation inhibition assay	835:868	biofilm formation inhibition assay	835:868	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	6	25	theme	formation	843:851	arg1	MIC					804:806	MIC	804:806	MIC	804:806	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	5	26	theme	released	752:759	arg1	ability					761:767	an excellent sustained released ability	729:767	an excellent sustained released ability (sustained release up to 180 min)	729:801	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	8	27	theme	motilities	1350:1359	arg1	inhibition					1295:1304	inhibition	1295:1304	inhibition of biofilm formation, swimming and swarming motilities	1295:1359	It could be inferred that the antibacterial activities against V. parahaemolyticus caused inhibition of biofilm formation, swimming and swarming motilities.					
37023879	1	28	theme	killer	191:196	arg1	"					197:197	the "Number one killer"	175:197	the "Number one killer" of seafood products	175:217	Vibrio parahemolyticus is the "Number one killer" of seafood products.					
37023879	1	28	theme	killer	191:196	arg1	parahemolyticus					156:170	Vibrio parahemolyticus	149:170	Vibrio parahemolyticus	149:170	Vibrio parahemolyticus is the "Number one killer" of seafood products.					
37023879	6	29	theme	strong	945:950	arg1	effects					1013:1019	strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects	945:1019	strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects	945:1019	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	8	30	theme	swarming	1341:1348	arg1	motilities					1350:1359	swarming motilities	1341:1359	swarming motilities	1341:1359	It could be inferred that the antibacterial activities against V. parahaemolyticus caused inhibition of biofilm formation, swimming and swarming motilities.					
37023879	9	31	theme	chitosan	1439:1446	arg1	agents					1462:1467	chitosan antibacterial agents	1439:1467	chitosan antibacterial agents	1439:1467	This study provided necessary data for the further design and development of chitosan antibacterial agents, food and feed additives.					
37023879	6	32	theme	inhibitory	1002:1011	arg1	effects					1013:1019	strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects	945:1019	strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects	945:1019	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	9	33	theme	antibacterial	1448:1460	arg1	agents					1462:1467	chitosan antibacterial agents	1439:1467	chitosan antibacterial agents	1439:1467	This study provided necessary data for the further design and development of chitosan antibacterial agents, food and feed additives.					
37023879	0	34	theme	antivibrio	21:30	arg1	activity					32:39	antivibrio activity	21:39	antivibrio activity	21:39	Characterization and antivibrio activity of chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent.					
37023879	4	35	theme	base/OH-CCa	581:591	arg1	structure					533:541	coordination structure	520:541	coordination structure	520:541	Additionally the coordination structure and morphology of Bridge-CS-CT-Schiff base/OH-CCa were verified.					
37023879	4	35	theme	base/OH-CCa	581:591	arg1	morphology					547:556	morphology	547:556	morphology	547:556	Additionally the coordination structure and morphology of Bridge-CS-CT-Schiff base/OH-CCa were verified.					
37023879	0	36	theme	antibacterial	128:140	arg1	agent					142:146	a calcium citrate sustained release antibacterial agent	92:146	a calcium citrate sustained release antibacterial agent	92:146	Characterization and antivibrio activity of chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent.					
37023879	6	37	theme	biofilm	835:841	arg1	assay					864:868	biofilm formation inhibition assay	835:868	biofilm formation inhibition assay	835:868	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	6	37	theme	biofilm	835:841	arg1	MIC					804:806	MIC	804:806	MIC	804:806	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	1	38	theme	seafood	202:208	arg1	products					210:217	seafood products	202:217	seafood products	202:217	Vibrio parahemolyticus is the "Number one killer" of seafood products.					
37023879	0	39	theme	chitosan-citral	44:58	arg1	complex					80:86	chitosan-citral Schiff base calcium complex	44:86	chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent	44:146	Characterization and antivibrio activity of chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent.					
37023879	4	40	theme	Bridge-CS-CT-Schiff	561:579	arg1	base/OH-CCa					581:591	Bridge-CS-CT-Schiff base/OH-CCa	561:591	Bridge-CS-CT-Schiff base/OH-CCa	561:591	Additionally the coordination structure and morphology of Bridge-CS-CT-Schiff base/OH-CCa were verified.					
37023879	8	41	theme	antibacterial	1235:1247	arg1	activities					1249:1258	the antibacterial activities	1231:1258	the antibacterial activities against V. parahaemolyticus	1231:1286	It could be inferred that the antibacterial activities against V. parahaemolyticus caused inhibition of biofilm formation, swimming and swarming motilities.					
37023879	2	42	theme	low	246:248	arg1	cost					250:253	low cost	246:253	low cost	246:253	Anti-vibrio agents having low cost and high-safety are urgently needed to supplement the application needs.					
37023879	1	43	theme	products	210:217	arg1	"					197:197	the "Number one killer"	175:197	the "Number one killer" of seafood products	175:217	Vibrio parahemolyticus is the "Number one killer" of seafood products.					
37023879	1	43	theme	products	210:217	arg1	parahemolyticus					156:170	Vibrio parahemolyticus	149:170	Vibrio parahemolyticus	149:170	Vibrio parahemolyticus is the "Number one killer" of seafood products.					
37023879	3	44	theme	calcium	413:419	arg1	CCa					430:432	CCa	430:432	CCa	430:432	This work attempted to prepare CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa) as raw material by microwave-assisted high-pressure homogenization.					
37023879	3	44	theme	calcium	413:419	arg1	citrate					421:427	calcium citrate	413:427	calcium citrate (CCa)	413:433	This work attempted to prepare CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa) as raw material by microwave-assisted high-pressure homogenization.					
37023879	4	45	theme	coordination	520:531	arg1	structure					533:541	coordination structure	520:541	coordination structure	520:541	Additionally the coordination structure and morphology of Bridge-CS-CT-Schiff base/OH-CCa were verified.					
37023879	5	46	dep	ability	761:767	arg1	release					780:786	sustained release	770:786	sustained release up to 180 min	770:800	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	9	47	theme	necessary	1382:1390	arg1	data					1392:1395	necessary data	1382:1395	necessary data for the further design and development of chitosan antibacterial agents, food and feed additives	1382:1492	This study provided necessary data for the further design and development of chitosan antibacterial agents, food and feed additives.					
37023879	3	48	theme	CS-CT-CCa	359:367	arg1	complex					369:375	CS-CT-CCa complex	359:375	CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa)	359:433	This work attempted to prepare CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa) as raw material by microwave-assisted high-pressure homogenization.					
37023879	0	49	theme	base	67:70	arg1	complex					80:86	chitosan-citral Schiff base calcium complex	44:86	chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent	44:146	Characterization and antivibrio activity of chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent.					
37023879	7	50	theme	biofilm-forming	1153:1167	arg1	ability					1169:1175	their biofilm-forming ability	1147:1175	their biofilm-forming ability	1147:1175	Meanwhile, CS-CT-CCa could increase the membrane permeability of V. parahaemolyticus and inhibit their biofilm-forming ability in a dose-dependent manner.					
37023879	6	51	theme	MDA	824:826	arg1	assay					828:832	MDA assay	824:832	MDA assay	824:832	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	6	51	theme	MDA	824:826	arg1	MIC					804:806	MIC	804:806	MIC	804:806	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	3	52	with	complex	369:375	arg1	CT					390:391	CT	390:391	CT	390:391	This work attempted to prepare CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa) as raw material by microwave-assisted high-pressure homogenization.					
37023879	3	52	with	complex	369:375	arg1	citrate					421:427	calcium citrate	413:427	calcium citrate (CCa)	413:433	This work attempted to prepare CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa) as raw material by microwave-assisted high-pressure homogenization.					
37023879	3	52	with	complex	369:375	arg1	chitosan					395:402	chitosan	395:402	chitosan (CS)	395:407	This work attempted to prepare CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa) as raw material by microwave-assisted high-pressure homogenization.					
37023879	3	52	with	complex	369:375	arg1	CS					405:406	CS	405:406	CS	405:406	This work attempted to prepare CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa) as raw material by microwave-assisted high-pressure homogenization.					
37023879	3	52	with	complex	369:375	arg1	CCa					430:432	CCa	430:432	CCa	430:432	This work attempted to prepare CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa) as raw material by microwave-assisted high-pressure homogenization.					
37023879	3	52	with	complex	369:375	arg1	citral					382:387	citral	382:387	citral (CT)	382:392	This work attempted to prepare CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa) as raw material by microwave-assisted high-pressure homogenization.					
37023879	6	53	dep	sustained	975:983	arg1	h					999:999	more than 12 h	986:999	more than 12 h	986:999	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	2	54	dep	application	309:319	arg1	needs					321:325	needs	321:325	needs	321:325	Anti-vibrio agents having low cost and high-safety are urgently needed to supplement the application needs.					
37023879	0	55	theme	Schiff	60:65	arg1	complex					80:86	chitosan-citral Schiff base calcium complex	44:86	chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent	44:146	Characterization and antivibrio activity of chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent.					
37023879	6	56	theme	motility	898:905	arg1	assay					907:911	swarming motility assay	889:911	swarming motility assay	889:911	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	6	56	theme	motility	898:905	arg1	MIC					804:806	MIC	804:806	MIC	804:806	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	7	57	theme	dose-dependent	1182:1195	arg1	manner					1197:1202	a dose-dependent manner	1180:1202	a dose-dependent manner	1180:1202	Meanwhile, CS-CT-CCa could increase the membrane permeability of V. parahaemolyticus and inhibit their biofilm-forming ability in a dose-dependent manner.					
37023879	2	58	theme	Anti-vibrio	220:230	arg1	agents					232:237	Anti-vibrio agents	220:237	Anti-vibrio agents having low cost and high-safety	220:269	Anti-vibrio agents having low cost and high-safety are urgently needed to supplement the application needs.					
37023879	3	59	theme	microwave-assisted	454:471	arg1	homogenization					487:500	microwave-assisted high-pressure homogenization	454:500	microwave-assisted high-pressure homogenization	454:500	This work attempted to prepare CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa) as raw material by microwave-assisted high-pressure homogenization.					
37023879	6	60	theme	swarming	889:896	arg1	assay					907:911	swarming motility assay	889:911	swarming motility assay	889:911	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	6	60	theme	swarming	889:896	arg1	MIC					804:806	MIC	804:806	MIC	804:806	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	5	61	theme	zeta	693:696	arg1	mV					721:722	the zeta potential: +38.7~+67.5 mV	689:722	the zeta potential: +38.7~+67.5 mV	689:722	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	9	62	dep	design	1413:1418	arg1	the					1401:1403	the	1401:1403	the	1401:1403	This study provided necessary data for the further design and development of chitosan antibacterial agents, food and feed additives.					
37023879	3	63	theme	raw	438:440	arg1	material					442:449	raw material	438:449	raw material	438:449	This work attempted to prepare CS-CT-CCa complex with citral (CT), chitosan (CS) and calcium citrate (CCa) as raw material by microwave-assisted high-pressure homogenization.					
37023879	5	64	theme	excellent	732:740	arg1	ability					761:767	an excellent sustained released ability	729:767	an excellent sustained released ability (sustained release up to 180 min)	729:801	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	5	65	theme	potential	698:706	arg1	mV					721:722	the zeta potential: +38.7~+67.5 mV	689:722	the zeta potential: +38.7~+67.5 mV	689:722	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	6	66	theme	Glucose	809:815	arg1	assay					817:821	Glucose assay	809:821	Glucose assay	809:821	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	6	66	theme	Glucose	809:815	arg1	MIC					804:806	MIC	804:806	MIC	804:806	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	5	67	dep	property	652:659	arg1	mV					721:722	the zeta potential: +38.7~+67.5 mV	689:722	the zeta potential: +38.7~+67.5 mV	689:722	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	5	67	dep	property	652:659	arg1	μm					682:683	the size: 3.55~9.33 μm	662:683	the size: 3.55~9.33 μm	662:683	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	1	68	theme	Vibrio	149:154	arg1	"					197:197	the "Number one killer"	175:197	the "Number one killer" of seafood products	175:217	Vibrio parahemolyticus is the "Number one killer" of seafood products.					
37023879	1	68	theme	Vibrio	149:154	arg1	parahemolyticus					156:170	Vibrio parahemolyticus	149:170	Vibrio parahemolyticus	149:170	Vibrio parahemolyticus is the "Number one killer" of seafood products.					
37023879	8	69	theme	biofilm	1309:1315	arg1	formation					1317:1325	biofilm formation	1309:1325	biofilm formation	1309:1325	It could be inferred that the antibacterial activities against V. parahaemolyticus caused inhibition of biofilm formation, swimming and swarming motilities.					
37023879	9	70	theme	additives	1484:1492	arg1	development					1424:1434	development	1424:1434	development	1424:1434	This study provided necessary data for the further design and development of chitosan antibacterial agents, food and feed additives.					
37023879	9	70	theme	additives	1484:1492	arg1	design					1413:1418	further design	1405:1418	further design	1405:1418	This study provided necessary data for the further design and development of chitosan antibacterial agents, food and feed additives.					
37023879	0	71	theme	complex	80:86	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and antivibrio activity of chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent.					
37023879	0	71	theme	complex	80:86	arg1	activity					32:39	antivibrio activity	21:39	antivibrio activity	21:39	Characterization and antivibrio activity of chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent.					
37023879	9	72	theme	food	1470:1473	arg1	development					1424:1434	development	1424:1434	development	1424:1434	This study provided necessary data for the further design and development of chitosan antibacterial agents, food and feed additives.					
37023879	9	72	theme	food	1470:1473	arg1	design					1413:1418	further design	1405:1418	further design	1405:1418	This study provided necessary data for the further design and development of chitosan antibacterial agents, food and feed additives.					
37023879	9	73	theme	feed	1479:1482	arg1	additives					1484:1492	feed additives	1479:1492	feed additives	1479:1492	This study provided necessary data for the further design and development of chitosan antibacterial agents, food and feed additives.					
37023879	5	74	contain	had	631:633	arg1	CS-CT-CCa					621:629	The prepared CS-CT-CCa	608:629	The prepared CS-CT-CCa	608:629	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	5	74	contain	had	631:633	arg2	ability					761:767	an excellent sustained released ability	729:767	an excellent sustained released ability (sustained release up to 180 min)	729:801	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	5	74	contain	had	631:633	arg2	property					652:659	a well-dispersed property	635:659	a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV)	635:723	The prepared CS-CT-CCa had a well-dispersed property (the size: 3.55~9.33 μm and the zeta potential: +38.7~+67.5 mV) and an excellent sustained released ability (sustained release up to 180 min).					
37023879	2	75	contain	having	239:244	arg2	high-safety					259:269	high-safety	259:269	high-safety	259:269	Anti-vibrio agents having low cost and high-safety are urgently needed to supplement the application needs.					
37023879	2	75	contain	having	239:244	arg1	agents					232:237	Anti-vibrio agents	220:237	Anti-vibrio agents having low cost and high-safety	220:269	Anti-vibrio agents having low cost and high-safety are urgently needed to supplement the application needs.					
37023879	2	75	contain	having	239:244	arg2	cost					250:253	low cost	246:253	low cost	246:253	Anti-vibrio agents having low cost and high-safety are urgently needed to supplement the application needs.					
37023879	0	76	theme	calcium	72:78	arg1	complex					80:86	chitosan-citral Schiff base calcium complex	44:86	chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent	44:146	Characterization and antivibrio activity of chitosan-citral Schiff base calcium complex for a calcium citrate sustained release antibacterial agent.					
37023879	7	77	theme	V.	1115:1116	arg1	parahaemolyticus					1118:1133	V. parahaemolyticus	1115:1133	V. parahaemolyticus	1115:1133	Meanwhile, CS-CT-CCa could increase the membrane permeability of V. parahaemolyticus and inhibit their biofilm-forming ability in a dose-dependent manner.					
37023879	6	78	theme	sustained	975:983	arg1	effects					1013:1019	strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects	945:1019	strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects	945:1019	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
37023879	6	79	theme	μg/ml	964:968	arg1	MIC					953:955	MIC	953:955	MIC of 128 μg/ml	953:968	MIC, Glucose assay, MDA assay, biofilm formation inhibition assay, SEM, swimming and swarming motility assay demonstrated that CS-CT-CCa had strong (MIC of 128 μg/ml) and sustained (more than 12 h) inhibitory effects against V. parahaemolyticus.					
36255219	0	0	theme	leaf	95:98	arg1	sucrose					100:106	leaf sucrose	95:106	leaf sucrose	95:106	Estimating intra-seasonal photosynthetic discrimination and water use efficiency using δ13C of leaf sucrose in Scots pine.					
36255219	1	1	contain	has	131:133	arg2	role					144:147	a unique role	135:147	a unique role	135:147	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	1	1	contain	has	131:133	arg1	Sucrose					123:129	Sucrose	123:129	Sucrose	123:129	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	2	2	theme	total	333:337	arg1	TOM					355:357	TOM	355:357	TOM	355:357	Yet, instead of sucrose, total organic matter (TOM) or water-soluble carbohydrates (WSC) are typically analysed in studies that follow δ13C signals within trees.					
36255219	2	2	theme	total	333:337	arg1	matter					347:352	total organic matter	333:352	total organic matter (TOM)	333:358	Yet, instead of sucrose, total organic matter (TOM) or water-soluble carbohydrates (WSC) are typically analysed in studies that follow δ13C signals within trees.					
36255219	3	3	theme	chamber-Picarro	722:736	arg1	δ13CA_Picarro					746:758	δ13CA_Picarro	746:758	δ13CA_Picarro	746:758	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	3	3	theme	chamber-Picarro	722:736	arg1	system					738:743	a chamber-Picarro system	720:743	a chamber-Picarro system (δ13CA_Picarro)	720:759	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	3	4	dep	Pinus	598:602	arg1	sylvestris					604:613	Pinus sylvestris	598:613	Pinus sylvestris	598:613	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	2	5	theme	δ13C	443:446	arg1	signals					448:454	δ13C signals	443:454	δ13C signals within trees	443:467	Yet, instead of sucrose, total organic matter (TOM) or water-soluble carbohydrates (WSC) are typically analysed in studies that follow δ13C signals within trees.					
36255219	6	6	from	δ13C	1288:1291	arg1	signals					1275:1281	deriving iWUE and environmental signals	1243:1281	deriving iWUE and environmental signals from δ13C of bulk organic matter	1243:1314	Thus, deriving iWUE and environmental signals from δ13C of bulk organic matter can lead to misinterpretation.					
36255219	5	7	from	superior	1057:1064	arg1	comparison					1000:1009	comparison	1000:1009	comparison with the other carbon pools	1000:1037	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	3	8	used	used	562:565	arg2	we					559:560	we	559:560	we	559:560	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	5	9	theme	water	1106:1110	arg1	iWUE					1128:1131	iWUE	1128:1131	iWUE	1128:1131	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	5	9	theme	water	1106:1110	arg1	efficiency					1116:1125	intrinsic water use efficiency	1096:1125	intrinsic water use efficiency (iWUE)	1096:1132	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	4	10	theme	δ13CA_Picarro	952:964	arg1	trends					923:928	seasonal trends	914:928	seasonal trends	914:928	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	4	10	theme	δ13CA_Picarro	952:964	arg1	values					942:947	absolute values	933:947	absolute values	933:947	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	5	11	theme	use	1112:1114	arg1	iWUE					1128:1131	iWUE	1128:1131	iWUE	1128:1131	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	5	11	theme	use	1112:1114	arg1	efficiency					1116:1125	intrinsic water use efficiency	1096:1125	intrinsic water use efficiency (iWUE)	1096:1132	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	0	12	theme	sucrose	100:106	arg1	δ13C					87:90	δ13C	87:90	δ13C of leaf sucrose in Scots pine	87:120	Estimating intra-seasonal photosynthetic discrimination and water use efficiency using δ13C of leaf sucrose in Scots pine.					
36255219	7	13	theme	physiological	1427:1439	arg1	responses					1441:1449	plant physiological responses	1421:1449	plant physiological responses in depth	1421:1458	Our findings underscore the advantage of using sucrose δ13C to understand plant physiological responses in depth.					
36255219	3	14	theme	organic	497:503	arg1	material					505:512	organic material	497:512	organic material	497:512	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	5	15	theme	gas	1223:1225	arg1	exchange					1227:1234	gas exchange	1223:1234	gas exchange	1223:1234	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	6	16	theme	deriving	1243:1250	arg1	signals					1275:1281	deriving iWUE and environmental signals	1243:1281	deriving iWUE and environmental signals from δ13C of bulk organic matter	1243:1314	Thus, deriving iWUE and environmental signals from δ13C of bulk organic matter can lead to misinterpretation.					
36255219	3	17	theme	different	654:662	arg1	pools					676:680	different leaf carbon pools	654:680	different leaf carbon pools	654:680	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	5	18	from	changes	1085:1091	arg1	iWUE					1128:1131	iWUE	1128:1131	iWUE	1128:1131	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	5	18	from	changes	1085:1091	arg1	efficiency					1116:1125	intrinsic water use efficiency	1096:1125	intrinsic water use efficiency (iWUE)	1096:1132	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	1	19	theme	environmental	162:174	arg1	signals					194:200	environmental and physiological signals	162:200	environmental and physiological signals	162:200	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	0	20	theme	Scots	111:115	arg1	pine					117:120	Scots pine	111:120	Scots pine	111:120	Estimating intra-seasonal photosynthetic discrimination and water use efficiency using δ13C of leaf sucrose in Scots pine.					
36255219	3	21	theme	time	641:644	arg1	δ13C					646:649	the first time δ13C	631:649	the first time δ13C of different leaf carbon pools	631:680	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	5	22	theme	carbon	1026:1031	arg1	pools					1033:1037	the other carbon pools	1016:1037	the other carbon pools	1016:1037	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	6	23	theme	matter	1309:1314	arg1	δ13C					1288:1291	δ13C	1288:1291	δ13C of bulk organic matter	1288:1314	Thus, deriving iWUE and environmental signals from δ13C of bulk organic matter can lead to misinterpretation.					
36255219	7	24	from	responses	1441:1449	arg1	depth					1454:1458	depth	1454:1458	depth	1454:1458	Our findings underscore the advantage of using sucrose δ13C to understand plant physiological responses in depth.					
36255219	0	25	theme	intra-seasonal	11:24	arg1	discrimination					41:54	intra-seasonal photosynthetic discrimination	11:54	intra-seasonal photosynthetic discrimination	11:54	Estimating intra-seasonal photosynthetic discrimination and water use efficiency using δ13C of leaf sucrose in Scots pine.					
36255219	6	26	theme	environmental	1261:1273	arg1	signals					1275:1281	deriving iWUE and environmental signals	1243:1281	deriving iWUE and environmental signals from δ13C of bulk organic matter	1243:1314	Thus, deriving iWUE and environmental signals from δ13C of bulk organic matter can lead to misinterpretation.					
36255219	4	27	dep	trends	923:928	arg1	the					910:912	the	910:912	the	910:912	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	5	28	dep	superior	1057:1064	arg1	agreeing					1135:1142	agreeing	1135:1142	agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange	1135:1234	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	1	29	theme	physiological	180:192	arg1	signals					194:200	environmental and physiological signals	162:200	environmental and physiological signals	162:200	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	4	30	theme	seasonal	914:921	arg1	trends					923:928	seasonal trends	914:928	seasonal trends	914:928	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	3	31	theme	pools	676:680	arg1	δ13C					646:649	the first time δ13C	631:649	the first time δ13C of different leaf carbon pools	631:680	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	4	32	theme	absolute	933:940	arg1	values					942:947	absolute values	933:947	absolute values	933:947	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	3	33	theme	material	505:512	arg1	choice					487:492	the choice	483:492	the choice of organic material	483:512	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	0	34	theme	photosynthetic	26:39	arg1	discrimination					41:54	intra-seasonal photosynthetic discrimination	11:54	intra-seasonal photosynthetic discrimination	11:54	Estimating intra-seasonal photosynthetic discrimination and water use efficiency using δ13C of leaf sucrose in Scots pine.					
36255219	1	35	theme	signal	286:291	arg1	composition					246:256	its carbon isotope composition	227:256	its carbon isotope composition (δ13C)	227:263	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	1	35	theme	signal	286:291	arg1	δ13C					259:262	δ13C	259:262	δ13C	259:262	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	1	35	theme	signal	286:291	arg1	transport					269:277	transport	269:277	transport of the signal to tree rings	269:305	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	6	36	theme	iWUE	1252:1255	arg1	signals					1275:1281	deriving iWUE and environmental signals	1243:1281	deriving iWUE and environmental signals from δ13C of bulk organic matter	1243:1314	Thus, deriving iWUE and environmental signals from δ13C of bulk organic matter can lead to misinterpretation.					
36255219	7	37	theme	plant	1421:1425	arg1	responses					1441:1449	plant physiological responses	1421:1449	plant physiological responses in depth	1421:1458	Our findings underscore the advantage of using sucrose δ13C to understand plant physiological responses in depth.					
36255219	0	38	theme	water	60:64	arg1	efficiency					70:79	water use efficiency	60:79	water use efficiency	60:79	Estimating intra-seasonal photosynthetic discrimination and water use efficiency using δ13C of leaf sucrose in Scots pine.					
36255219	3	39	theme	carbon	669:674	arg1	pools					676:680	different leaf carbon pools	654:680	different leaf carbon pools	654:680	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	4	40	theme	tested	852:857	arg1	TOM					881:883	TOM	881:883	TOM	881:883	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	4	40	theme	tested	852:857	arg1	pools					866:870	the other tested carbon pools	842:870	the other tested carbon pools	842:870	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	4	40	theme	tested	852:857	arg1	WSC					889:891	WSC	889:891	WSC	889:891	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	3	41	theme	δ13C	545:548	arg1	records					550:556	δ13C records	545:556	δ13C records	545:556	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	5	42	with	comparison	1000:1009	arg1	pools					1033:1037	the other carbon pools	1016:1037	the other carbon pools	1016:1037	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	6	43	theme	organic	1301:1307	arg1	matter					1309:1314	bulk organic matter	1296:1314	bulk organic matter	1296:1314	Thus, deriving iWUE and environmental signals from δ13C of bulk organic matter can lead to misinterpretation.					
36255219	1	44	theme	tree	296:299	arg1	rings					301:305	tree rings	296:305	tree rings	296:305	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	4	45	theme	other	846:850	arg1	TOM					881:883	TOM	881:883	TOM	881:883	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	4	45	theme	other	846:850	arg1	pools					866:870	the other tested carbon pools	842:870	the other tested carbon pools	842:870	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	4	45	theme	other	846:850	arg1	WSC					889:891	WSC	889:891	WSC	889:891	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	0	46	from	δ13C	87:90	arg1	pine					117:120	Scots pine	111:120	Scots pine	111:120	Estimating intra-seasonal photosynthetic discrimination and water use efficiency using δ13C of leaf sucrose in Scots pine.					
36255219	3	47	theme	records	550:556	arg1	interpretation					527:540	the interpretation	523:540	the interpretation of δ13C records	523:556	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	2	48	theme	water-soluble	363:375	arg1	carbohydrates					377:389	water-soluble carbohydrates	363:389	water-soluble carbohydrates (WSC)	363:395	Yet, instead of sucrose, total organic matter (TOM) or water-soluble carbohydrates (WSC) are typically analysed in studies that follow δ13C signals within trees.					
36255219	2	48	theme	water-soluble	363:375	arg1	WSC					392:394	WSC	392:394	WSC	392:394	Yet, instead of sucrose, total organic matter (TOM) or water-soluble carbohydrates (WSC) are typically analysed in studies that follow δ13C signals within trees.					
36255219	6	49	theme	bulk	1296:1299	arg1	matter					1309:1314	bulk organic matter	1296:1314	bulk organic matter	1296:1314	Thus, deriving iWUE and environmental signals from δ13C of bulk organic matter can lead to misinterpretation.					
36255219	3	50	theme	assimilates	695:705	arg1	δ13C					687:690	δ13C	687:690	δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model)	687:816	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	3	51	theme	Scots	586:590	arg1	pine					592:595	mature field-grown Scots pine	567:595	mature field-grown Scots pine (Pinus sylvestris)	567:614	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	3	51	theme	Scots	586:590	arg1	Pinus					598:602	Pinus	598:602	Pinus	598:602	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	0	52	theme	use	66:68	arg1	efficiency					70:79	water use efficiency	60:79	water use efficiency	60:79	Estimating intra-seasonal photosynthetic discrimination and water use efficiency using δ13C of leaf sucrose in Scots pine.					
36255219	5	53	theme	sucrose	1040:1046	arg1	δ13C					1048:1051	sucrose δ13C	1040:1051	sucrose δ13C	1040:1051	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	3	54	theme	field-grown	574:584	arg1	pine					592:595	mature field-grown Scots pine	567:595	mature field-grown Scots pine (Pinus sylvestris)	567:614	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	3	54	theme	field-grown	574:584	arg1	Pinus					598:602	Pinus	598:602	Pinus	598:602	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	3	55	theme	photosynthetic	768:781	arg1	δ13CA_model					805:815	δ13CA_model	805:815	δ13CA_model	805:815	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	3	55	theme	photosynthetic	768:781	arg1	model					798:802	a photosynthetic discrimination model	766:802	a photosynthetic discrimination model (δ13CA_model)	766:816	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	1	56	from	photosynthesis	209:222	arg1	composition					246:256	its carbon isotope composition	227:256	its carbon isotope composition (δ13C)	227:263	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	1	56	from	photosynthesis	209:222	arg1	δ13C					259:262	δ13C	259:262	δ13C	259:262	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	1	56	from	photosynthesis	209:222	arg1	transport					269:277	transport	269:277	transport of the signal to tree rings	269:305	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	3	57	theme	leaf	664:667	arg1	pools					676:680	different leaf carbon pools	654:680	different leaf carbon pools	654:680	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	3	58	theme	discrimination	783:796	arg1	δ13CA_model					805:815	δ13CA_model	805:815	δ13CA_model	805:815	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	3	58	theme	discrimination	783:796	arg1	model					798:802	a photosynthetic discrimination model	766:802	a photosynthetic discrimination model (δ13CA_model)	766:816	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	1	59	theme	carbon	231:236	arg1	composition					246:256	its carbon isotope composition	227:256	its carbon isotope composition (δ13C)	227:263	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	1	59	theme	carbon	231:236	arg1	δ13C					259:262	δ13C	259:262	δ13C	259:262	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	7	60	theme	sucrose	1394:1400	arg1	δ13C					1402:1405	sucrose δ13C	1394:1405	sucrose δ13C	1394:1405	Our findings underscore the advantage of using sucrose δ13C to understand plant physiological responses in depth.					
36255219	3	61	theme	mature	567:572	arg1	pine					592:595	mature field-grown Scots pine	567:595	mature field-grown Scots pine (Pinus sylvestris)	567:614	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	3	61	theme	mature	567:572	arg1	Pinus					598:602	Pinus	598:602	Pinus	598:602	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	5	62	theme	intrinsic	1096:1104	arg1	iWUE					1128:1131	iWUE	1128:1131	iWUE	1128:1131	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	5	62	theme	intrinsic	1096:1104	arg1	efficiency					1116:1125	intrinsic water use efficiency	1096:1125	intrinsic water use efficiency (iWUE)	1096:1132	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	5	63	theme	intra-seasonal	1172:1185	arg1	variations					1187:1196	intra-seasonal variations	1172:1196	intra-seasonal variations	1172:1196	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	1	64	theme	isotope	238:244	arg1	composition					246:256	its carbon isotope composition	227:256	its carbon isotope composition (δ13C)	227:263	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	1	64	theme	isotope	238:244	arg1	δ13C					259:262	δ13C	259:262	δ13C	259:262	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	5	65	theme	absolute	1152:1159	arg1	values					1161:1166	absolute values	1152:1166	absolute values	1152:1166	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	1	66	theme	unique	137:142	arg1	role					144:147	a unique role	135:147	a unique role	135:147	Sucrose has a unique role in recording environmental and physiological signals during photosynthesis in its carbon isotope composition (δ13C) and transport of the signal to tree rings.					
36255219	4	67	theme	δ13CA_model	970:980	arg1	trends					923:928	seasonal trends	914:928	seasonal trends	914:928	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	4	67	theme	δ13CA_model	970:980	arg1	values					942:947	absolute values	933:947	absolute values	933:947	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	5	68	theme	other	1020:1024	arg1	pools					1033:1037	the other carbon pools	1016:1037	the other carbon pools	1016:1037	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	5	69	from	comparison	1000:1009	arg1	superior					1057:1064	superior	1057:1064	superior	1057:1064	Consequently, in comparison with the other carbon pools, sucrose δ13C was superior for reconstructing changes in intrinsic water use efficiency (iWUE), agreeing in both absolute values and intra-seasonal variations with iWUE estimated from gas exchange.					
36255219	3	70	theme	first	635:639	arg1	δ13C					646:649	the first time δ13C	631:649	the first time δ13C of different leaf carbon pools	631:680	To study how the choice of organic material may bias the interpretation of δ13C records, we used mature field-grown Scots pine (Pinus sylvestris) to compare for the first time δ13C of different leaf carbon pools with δ13C of assimilates estimated by a chamber-Picarro system (δ13CA_Picarro), and a photosynthetic discrimination model (δ13CA_model).					
36255219	2	71	theme	organic	339:345	arg1	TOM					355:357	TOM	355:357	TOM	355:357	Yet, instead of sucrose, total organic matter (TOM) or water-soluble carbohydrates (WSC) are typically analysed in studies that follow δ13C signals within trees.					
36255219	2	71	theme	organic	339:345	arg1	matter					347:352	total organic matter	333:352	total organic matter (TOM)	333:358	Yet, instead of sucrose, total organic matter (TOM) or water-soluble carbohydrates (WSC) are typically analysed in studies that follow δ13C signals within trees.					
36255219	4	72	theme	carbon	859:864	arg1	TOM					881:883	TOM	881:883	TOM	881:883	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	4	72	theme	carbon	859:864	arg1	pools					866:870	the other tested carbon pools	842:870	the other tested carbon pools	842:870	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36255219	4	72	theme	carbon	859:864	arg1	WSC					889:891	WSC	889:891	WSC	889:891	Compared with sucrose, the other tested carbon pools, such as TOM and WSC, poorly recorded the seasonal trends or absolute values of δ13CA_Picarro and δ13CA_model.					
36512950	3	0	theme	Alg/Gel/mMWCNTs	558:572	arg1	scaffolds					585:593	The Alg/Gel/mMWCNTs conductive scaffolds	554:593	The Alg/Gel/mMWCNTs conductive scaffolds with mMWCNTs content of 1%, 3%, and 5%	554:632	The Alg/Gel/mMWCNTs conductive scaffolds with mMWCNTs content of 1%, 3%, and 5% were prepared by freeze-drying, respectively.					
36512950	1	1	theme	neural	262:267	arg1	engineering					276:286	neural tissue engineering	262:286	neural tissue engineering	262:286	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	10	2	theme	Alg/Gel/mMWCNTs	1644:1658	arg1	scaffold					1671:1678	the Alg/Gel/mMWCNTs conductive scaffold	1640:1678	the Alg/Gel/mMWCNTs conductive scaffold	1640:1678	Therefore, the Alg/Gel/mMWCNTs conductive scaffold has potential as a tissue engineering scaffold in nerve regeneration.					
36512950	3	3	with	scaffolds	585:593	arg1	content					608:614	mMWCNTs content	600:614	mMWCNTs content of 1%, 3%, and 5%	600:632	The Alg/Gel/mMWCNTs conductive scaffolds with mMWCNTs content of 1%, 3%, and 5% were prepared by freeze-drying, respectively.					
36512950	5	4	theme	electron	884:891	arg1	SEM					905:907	SEM	905:907	SEM	905:907	The conductive scaffolds were characterized by Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).					
36512950	5	4	theme	electron	884:891	arg1	microscopy					893:902	Scanning electron microscopy	875:902	Scanning electron microscopy (SEM)	875:908	The conductive scaffolds were characterized by Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).					
36512950	3	5	theme	mMWCNTs	600:606	arg1	content					608:614	mMWCNTs content	600:614	mMWCNTs content of 1%, 3%, and 5%	600:632	The Alg/Gel/mMWCNTs conductive scaffolds with mMWCNTs content of 1%, 3%, and 5% were prepared by freeze-drying, respectively.					
36512950	6	6	theme	culture	1153:1159	arg1	time					1161:1164	culture time	1153:1164	culture time	1153:1164	In general, the mMWCNTs addition improved the hydrophilic, electrical conductivity and mechanical properties of the composite scaffold, and PC12 cells showed a trend of gradual increase over culture time.					
36512950	8	7	theme	surface	1267:1273	arg1	angle					1283:1287	the surface contact angle	1263:1287	the surface contact angle	1263:1287	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	0	8	theme	neural	142:147	arg1	engineering					156:166	neural tissue engineering	142:166	neural tissue engineering	142:166	Fabrication and detection of a novel hybrid conductive scaffold based on alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering.					
36512950	3	9	theme	%	620:620	arg1	content					608:614	mMWCNTs content	600:614	mMWCNTs content of 1%, 3%, and 5%	600:632	The Alg/Gel/mMWCNTs conductive scaffolds with mMWCNTs content of 1%, 3%, and 5% were prepared by freeze-drying, respectively.					
36512950	1	10	theme	conductive	206:215	arg1	nanomaterials					224:236	conductive carbon nanomaterials	206:236	conductive carbon nanomaterials	206:236	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	8	11	theme	59.77 ± 0.94	1476:1487	arg1	%					1471:1471	%	1471:1471	%	1471:1471	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	7	12	theme	proliferation	1230:1242	arg1	behavior					1244:1251	the best cell proliferation behavior	1216:1251	the best cell proliferation behavior	1216:1251	Particularly, the Alg/Gel-1%C scaffold exhibited the best cell proliferation behavior.					
36512950	8	13	theme	G2	1498:1499	arg1	S/cm					1414:1417	1.32 × 10-3 ± 2.1 × 10-4 S/cm	1389:1417	1.32 × 10-3 ± 2.1 × 10-4 S/cm	1389:1417	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	13	theme	G2	1498:1499	arg1	phase					1501:1505	the G2 phase	1494:1505	the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively	1494:1554	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	14	theme	electrical	1328:1337	arg1	conductivity					1339:1350	the electrical conductivity	1324:1350	the electrical conductivity	1324:1350	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	7	15	theme	best	1220:1223	arg1	behavior					1244:1251	the best cell proliferation behavior	1216:1251	the best cell proliferation behavior	1216:1251	Particularly, the Alg/Gel-1%C scaffold exhibited the best cell proliferation behavior.					
36512950	2	16	theme	hybrid	536:541	arg1	scaffolds					543:551	the hybrid scaffolds	532:551	the hybrid scaffolds	532:551	In this study, the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs) were introduced into sodium alginate/gelatin (Alg/Gel) scaffolds to optimize the function of the hybrid scaffolds.					
36512950	4	17	theme	scaffolds	762:770	arg1	biocompatibility					731:746	biocompatibility	731:746	biocompatibility of the hybrid scaffolds at different magnetic field intensities	731:810	Following this, the physicochemical properties and biocompatibility of the hybrid scaffolds at different magnetic field intensities were evaluated.					
36512950	4	17	theme	scaffolds	762:770	arg1	properties					716:725	the physicochemical properties	696:725	the physicochemical properties	696:725	Following this, the physicochemical properties and biocompatibility of the hybrid scaffolds at different magnetic field intensities were evaluated.					
36512950	0	18	theme	carbon	103:108	arg1	nanotubes					110:118	alginate/gelatin/carboxylated carbon nanotubes	73:118	alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering	73:166	Fabrication and detection of a novel hybrid conductive scaffold based on alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering.					
36512950	0	18	theme	carbon	103:108	arg1	Alg/Gel/mMWCNTs					121:135	Alg/Gel/mMWCNTs	121:135	Alg/Gel/mMWCNTs	121:135	Fabrication and detection of a novel hybrid conductive scaffold based on alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering.					
36512950	4	19	theme	different	775:783	arg1	intensities					800:810	different magnetic field intensities	775:810	different magnetic field intensities	775:810	Following this, the physicochemical properties and biocompatibility of the hybrid scaffolds at different magnetic field intensities were evaluated.					
36512950	2	20	theme	carbon	412:417	arg1	mMWCNTs					430:436	mMWCNTs	430:436	mMWCNTs	430:436	In this study, the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs) were introduced into sodium alginate/gelatin (Alg/Gel) scaffolds to optimize the function of the hybrid scaffolds.					
36512950	2	20	theme	carbon	412:417	arg1	nanotubes					419:427	the carboxyl-modified multi-walled carbon nanotubes	377:427	the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs)	377:437	In this study, the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs) were introduced into sodium alginate/gelatin (Alg/Gel) scaffolds to optimize the function of the hybrid scaffolds.					
36512950	7	21	theme	C	1195:1195	arg1	scaffold					1197:1204	the Alg/Gel-1%C scaffold	1181:1204	the Alg/Gel-1%C scaffold	1181:1204	Particularly, the Alg/Gel-1%C scaffold exhibited the best cell proliferation behavior.					
36512950	0	22	theme	novel	31:35	arg1	scaffold					55:62	a novel hybrid conductive scaffold	29:62	a novel hybrid conductive scaffold	29:62	Fabrication and detection of a novel hybrid conductive scaffold based on alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering.					
36512950	2	23	theme	sodium	460:465	arg1	Alg/Gel					485:491	Alg/Gel	485:491	Alg/Gel	485:491	In this study, the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs) were introduced into sodium alginate/gelatin (Alg/Gel) scaffolds to optimize the function of the hybrid scaffolds.					
36512950	2	23	theme	sodium	460:465	arg1	alginate/gelatin					467:482	sodium alginate/gelatin	460:482	sodium alginate/gelatin (Alg/Gel) scaffolds	460:502	In this study, the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs) were introduced into sodium alginate/gelatin (Alg/Gel) scaffolds to optimize the function of the hybrid scaffolds.					
36512950	8	24	theme	13.93 ± 1.26	1529:1540	arg1	%					1524:1524	%	1524:1524	%	1524:1524	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	2	25	theme	carboxyl-modified	381:397	arg1	mMWCNTs					430:436	mMWCNTs	430:436	mMWCNTs	430:436	In this study, the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs) were introduced into sodium alginate/gelatin (Alg/Gel) scaffolds to optimize the function of the hybrid scaffolds.					
36512950	2	25	theme	carboxyl-modified	381:397	arg1	nanotubes					419:427	the carboxyl-modified multi-walled carbon nanotubes	377:427	the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs)	377:437	In this study, the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs) were introduced into sodium alginate/gelatin (Alg/Gel) scaffolds to optimize the function of the hybrid scaffolds.					
36512950	7	26	theme	Alg/Gel-1	1185:1193	arg1	scaffold					1197:1204	the Alg/Gel-1%C scaffold	1181:1204	the Alg/Gel-1%C scaffold	1181:1204	Particularly, the Alg/Gel-1%C scaffold exhibited the best cell proliferation behavior.					
36512950	1	27	theme	tissue	269:274	arg1	engineering					276:286	neural tissue engineering	262:286	neural tissue engineering	262:286	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	9	28	from	well-shaped	1592:1602	arg1	images					1568:1573	the SEM images	1560:1573	the SEM images	1560:1573	In the SEM images, PC12 cells were well-shaped and densely distributed.					
36512950	6	29	theme	composite	1078:1086	arg1	scaffold					1088:1095	the composite scaffold	1074:1095	the composite scaffold	1074:1095	In general, the mMWCNTs addition improved the hydrophilic, electrical conductivity and mechanical properties of the composite scaffold, and PC12 cells showed a trend of gradual increase over culture time.					
36512950	8	30	theme	± 2.1 × 10-4	1401:1412	arg1	S/cm					1414:1417	1.32 × 10-3 ± 2.1 × 10-4 S/cm	1389:1417	1.32 × 10-3 ± 2.1 × 10-4 S/cm	1389:1417	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	30	theme	± 2.1 × 10-4	1401:1412	arg1	phase					1501:1505	the G2 phase	1494:1505	the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively	1494:1554	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	30	theme	± 2.1 × 10-4	1401:1412	arg1	phase					1448:1452	the G1 phase	1441:1452	the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94%	1441:1488	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	5	31	theme	conductive	832:841	arg1	scaffolds					843:851	The conductive scaffolds	828:851	The conductive scaffolds	828:851	The conductive scaffolds were characterized by Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).					
36512950	9	32	theme	PC12	1576:1579	arg1	cells					1581:1585	PC12 cells	1576:1585	PC12 cells	1576:1585	In the SEM images, PC12 cells were well-shaped and densely distributed.					
36512950	6	33	theme	mechanical	1049:1058	arg1	properties					1060:1069	the hydrophilic, electrical conductivity and mechanical properties	1004:1069	properties	1060:1069	In general, the mMWCNTs addition improved the hydrophilic, electrical conductivity and mechanical properties of the composite scaffold, and PC12 cells showed a trend of gradual increase over culture time.					
36512950	0	34	theme	scaffold	55:62	arg1	detection					16:24	detection	16:24	detection	16:24	Fabrication and detection of a novel hybrid conductive scaffold based on alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering.					
36512950	0	34	theme	scaffold	55:62	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and detection of a novel hybrid conductive scaffold based on alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering.					
36512950	0	35	theme	hybrid	37:42	arg1	scaffold					55:62	a novel hybrid conductive scaffold	29:62	a novel hybrid conductive scaffold	29:62	Fabrication and detection of a novel hybrid conductive scaffold based on alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering.					
36512950	1	36	theme	electrical	311:320	arg1	conductivity					322:333	their excellent electrical conductivity	295:333	their excellent electrical conductivity	295:333	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	8	37	dep	60 ± 3°	1315:1321	arg1	to					1312:1313	to	1312:1313	to	1312:1313	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	6	38	theme	gradual	1131:1137	arg1	increase					1139:1146	gradual increase	1131:1146	gradual increase over culture time	1131:1164	In general, the mMWCNTs addition improved the hydrophilic, electrical conductivity and mechanical properties of the composite scaffold, and PC12 cells showed a trend of gradual increase over culture time.					
36512950	4	39	theme	field	794:798	arg1	intensities					800:810	different magnetic field intensities	775:810	different magnetic field intensities	775:810	Following this, the physicochemical properties and biocompatibility of the hybrid scaffolds at different magnetic field intensities were evaluated.					
36512950	10	40	theme	tissue	1699:1704	arg1	engineering					1706:1716	tissue engineering	1699:1716	a tissue engineering scaffold in nerve regeneration	1697:1747	Therefore, the Alg/Gel/mMWCNTs conductive scaffold has potential as a tissue engineering scaffold in nerve regeneration.					
36512950	1	41	theme	carbon	217:222	arg1	nanomaterials					224:236	conductive carbon nanomaterials	206:236	conductive carbon nanomaterials	206:236	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	8	42	dep	%	1471:1471	arg1	to					1473:1474	to	1473:1474	to	1473:1474	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	10	43	theme	nerve	1730:1734	arg1	regeneration					1736:1747	nerve regeneration	1730:1747	nerve regeneration	1730:1747	Therefore, the Alg/Gel/mMWCNTs conductive scaffold has potential as a tissue engineering scaffold in nerve regeneration.					
36512950	6	44	theme	PC12	1102:1105	arg1	cells					1107:1111	PC12 cells	1102:1111	PC12 cells	1102:1111	In general, the mMWCNTs addition improved the hydrophilic, electrical conductivity and mechanical properties of the composite scaffold, and PC12 cells showed a trend of gradual increase over culture time.					
36512950	10	45	from	scaffold	1718:1725	arg1	regeneration					1736:1747	nerve regeneration	1730:1747	nerve regeneration	1730:1747	Therefore, the Alg/Gel/mMWCNTs conductive scaffold has potential as a tissue engineering scaffold in nerve regeneration.					
36512950	5	46	theme	Scanning	875:882	arg1	SEM					905:907	SEM	905:907	SEM	905:907	The conductive scaffolds were characterized by Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).					
36512950	5	46	theme	Scanning	875:882	arg1	microscopy					893:902	Scanning electron microscopy	875:902	Scanning electron microscopy (SEM)	875:908	The conductive scaffolds were characterized by Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).					
36512950	10	47	contain	has	1680:1682	arg2	potential					1684:1692	potential	1684:1692	potential	1684:1692	Therefore, the Alg/Gel/mMWCNTs conductive scaffold has potential as a tissue engineering scaffold in nerve regeneration.					
36512950	10	47	contain	has	1680:1682	arg2	scaffold					1718:1725	a tissue engineering scaffold	1697:1725	a tissue engineering scaffold in nerve regeneration	1697:1747	Therefore, the Alg/Gel/mMWCNTs conductive scaffold has potential as a tissue engineering scaffold in nerve regeneration.					
36512950	10	47	contain	has	1680:1682	arg1	scaffold					1671:1678	the Alg/Gel/mMWCNTs conductive scaffold	1640:1678	the Alg/Gel/mMWCNTs conductive scaffold	1640:1678	Therefore, the Alg/Gel/mMWCNTs conductive scaffold has potential as a tissue engineering scaffold in nerve regeneration.					
36512950	8	48	theme	10.32 ± 0.35	1512:1523	arg1	%					1524:1524	%	1524:1524	%	1524:1524	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	6	49	theme	mMWCNTs	978:984	arg1	addition					986:993	the mMWCNTs addition	974:993	the mMWCNTs addition	974:993	In general, the mMWCNTs addition improved the hydrophilic, electrical conductivity and mechanical properties of the composite scaffold, and PC12 cells showed a trend of gradual increase over culture time.					
36512950	3	50	theme	conductive	574:583	arg1	scaffolds					585:593	The Alg/Gel/mMWCNTs conductive scaffolds	554:593	The Alg/Gel/mMWCNTs conductive scaffolds with mMWCNTs content of 1%, 3%, and 5%	554:632	The Alg/Gel/mMWCNTs conductive scaffolds with mMWCNTs content of 1%, 3%, and 5% were prepared by freeze-drying, respectively.					
36512950	1	51	theme	as	194:195	arg1	kinds					197:201	as kinds	194:201	as kinds of conductive carbon nanomaterials	194:236	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	1	51	theme	as	194:195	arg1	nanotubes					176:184	Carbon nanotubes	169:184	Carbon nanotubes (CNTs)	169:191	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	8	52	theme	55.67 ± 1.86	1459:1470	arg1	%					1471:1471	%	1471:1471	%	1471:1471	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	0	53	theme	tissue	149:154	arg1	engineering					156:166	neural tissue engineering	142:166	neural tissue engineering	142:166	Fabrication and detection of a novel hybrid conductive scaffold based on alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering.					
36512950	2	54	theme	scaffolds	543:551	arg1	function					520:527	the function	516:527	the function of the hybrid scaffolds	516:551	In this study, the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs) were introduced into sodium alginate/gelatin (Alg/Gel) scaffolds to optimize the function of the hybrid scaffolds.					
36512950	8	55	theme	contact	1275:1281	arg1	angle					1283:1287	the surface contact angle	1263:1287	the surface contact angle	1263:1287	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	3	56	theme	%	624:624	arg1	content					608:614	mMWCNTs content	600:614	mMWCNTs content of 1%, 3%, and 5%	600:632	The Alg/Gel/mMWCNTs conductive scaffolds with mMWCNTs content of 1%, 3%, and 5% were prepared by freeze-drying, respectively.					
36512950	4	57	theme	physicochemical	700:714	arg1	properties					716:725	the physicochemical properties	696:725	the physicochemical properties	696:725	Following this, the physicochemical properties and biocompatibility of the hybrid scaffolds at different magnetic field intensities were evaluated.					
36512950	7	58	theme	cell	1225:1228	arg1	behavior					1244:1251	the best cell proliferation behavior	1216:1251	the best cell proliferation behavior	1216:1251	Particularly, the Alg/Gel-1%C scaffold exhibited the best cell proliferation behavior.					
36512950	4	59	from	intensities	800:810	arg1	biocompatibility					731:746	biocompatibility	731:746	biocompatibility of the hybrid scaffolds at different magnetic field intensities	731:810	Following this, the physicochemical properties and biocompatibility of the hybrid scaffolds at different magnetic field intensities were evaluated.					
36512950	4	59	from	intensities	800:810	arg1	properties					716:725	the physicochemical properties	696:725	the physicochemical properties	696:725	Following this, the physicochemical properties and biocompatibility of the hybrid scaffolds at different magnetic field intensities were evaluated.					
36512950	9	60	from	images	1568:1573	arg1	well-shaped					1592:1602	well-shaped	1592:1602	well-shaped	1592:1602	In the SEM images, PC12 cells were well-shaped and densely distributed.					
36512950	0	61	theme	alginate/gelatin/carboxylated	73:101	arg1	nanotubes					110:118	alginate/gelatin/carboxylated carbon nanotubes	73:118	alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering	73:166	Fabrication and detection of a novel hybrid conductive scaffold based on alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering.					
36512950	0	61	theme	alginate/gelatin/carboxylated	73:101	arg1	Alg/Gel/mMWCNTs					121:135	Alg/Gel/mMWCNTs	121:135	Alg/Gel/mMWCNTs	121:135	Fabrication and detection of a novel hybrid conductive scaffold based on alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering.					
36512950	1	62	theme	good	339:342	arg1	biocompatibility					344:359	good biocompatibility	339:359	good biocompatibility	339:359	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	1	63	theme	Carbon	169:174	arg1	kinds					197:201	as kinds	194:201	as kinds of conductive carbon nanomaterials	194:236	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	1	63	theme	Carbon	169:174	arg1	CNTs					187:190	CNTs	187:190	CNTs	187:190	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	1	63	theme	Carbon	169:174	arg1	nanotubes					176:184	Carbon nanotubes	169:184	Carbon nanotubes (CNTs)	169:191	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	6	64	theme	hydrophilic	1008:1018	arg1	conductivity					1032:1043	the hydrophilic, electrical conductivity and mechanical properties	1004:1069	conductivity	1032:1043	In general, the mMWCNTs addition improved the hydrophilic, electrical conductivity and mechanical properties of the composite scaffold, and PC12 cells showed a trend of gradual increase over culture time.					
36512950	6	65	dep	hydrophilic	1008:1018	arg1	electrical					1021:1030	electrical	1021:1030	electrical	1021:1030	In general, the mMWCNTs addition improved the hydrophilic, electrical conductivity and mechanical properties of the composite scaffold, and PC12 cells showed a trend of gradual increase over culture time.					
36512950	4	66	theme	hybrid	755:760	arg1	scaffolds					762:770	the hybrid scaffolds	751:770	the hybrid scaffolds	751:770	Following this, the physicochemical properties and biocompatibility of the hybrid scaffolds at different magnetic field intensities were evaluated.					
36512950	8	67	from	%	1488:1488	arg1	S/cm					1414:1417	1.32 × 10-3 ± 2.1 × 10-4 S/cm	1389:1417	1.32 × 10-3 ± 2.1 × 10-4 S/cm	1389:1417	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	67	from	%	1488:1488	arg1	phase					1501:1505	the G2 phase	1494:1505	the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively	1494:1554	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	67	from	%	1488:1488	arg1	phase					1448:1452	the G1 phase	1441:1452	the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94%	1441:1488	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	68	theme	compressive	1356:1366	arg1	modulus					1368:1374	compressive modulus	1356:1374	compressive modulus	1356:1374	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	7	69	theme	%	1194:1194	arg1	scaffold					1197:1204	the Alg/Gel-1%C scaffold	1181:1204	the Alg/Gel-1%C scaffold	1181:1204	Particularly, the Alg/Gel-1%C scaffold exhibited the best cell proliferation behavior.					
36512950	9	70	theme	SEM	1564:1566	arg1	images					1568:1573	the SEM images	1560:1573	the SEM images	1560:1573	In the SEM images, PC12 cells were well-shaped and densely distributed.					
36512950	6	71	theme	scaffold	1088:1095	arg1	conductivity					1032:1043	the hydrophilic, electrical conductivity and mechanical properties	1004:1069	conductivity	1032:1043	In general, the mMWCNTs addition improved the hydrophilic, electrical conductivity and mechanical properties of the composite scaffold, and PC12 cells showed a trend of gradual increase over culture time.					
36512950	6	71	theme	scaffold	1088:1095	arg1	properties					1060:1069	the hydrophilic, electrical conductivity and mechanical properties	1004:1069	properties	1060:1069	In general, the mMWCNTs addition improved the hydrophilic, electrical conductivity and mechanical properties of the composite scaffold, and PC12 cells showed a trend of gradual increase over culture time.					
36512950	5	72	dep	Fourier	914:920	arg1	transform					922:930	transform	922:930	transform infrared spectroscopy (FTIR)	922:959	The conductive scaffolds were characterized by Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).					
36512950	5	73	dep	transform	922:930	arg1	infrared					932:939	infrared	932:939	transform infrared spectroscopy (FTIR)	922:959	The conductive scaffolds were characterized by Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).					
36512950	3	74	theme	%	632:632	arg1	content					608:614	mMWCNTs content	600:614	mMWCNTs content of 1%, 3%, and 5%	600:632	The Alg/Gel/mMWCNTs conductive scaffolds with mMWCNTs content of 1%, 3%, and 5% were prepared by freeze-drying, respectively.					
36512950	8	75	theme	1.32 × 10-3	1389:1399	arg1	S/cm					1414:1417	1.32 × 10-3 ± 2.1 × 10-4 S/cm	1389:1417	1.32 × 10-3 ± 2.1 × 10-4 S/cm	1389:1417	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	75	theme	1.32 × 10-3	1389:1399	arg1	phase					1501:1505	the G2 phase	1494:1505	the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively	1494:1554	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	75	theme	1.32 × 10-3	1389:1399	arg1	phase					1448:1452	the G1 phase	1441:1452	the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94%	1441:1488	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	76	from	%	1541:1541	arg1	S/cm					1414:1417	1.32 × 10-3 ± 2.1 × 10-4 S/cm	1389:1417	1.32 × 10-3 ± 2.1 × 10-4 S/cm	1389:1417	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	76	from	%	1541:1541	arg1	phase					1501:1505	the G2 phase	1494:1505	the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively	1494:1554	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	76	from	%	1541:1541	arg1	phase					1448:1452	the G1 phase	1441:1452	the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94%	1441:1488	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	10	77	theme	conductive	1660:1669	arg1	scaffold					1671:1678	the Alg/Gel/mMWCNTs conductive scaffold	1640:1678	the Alg/Gel/mMWCNTs conductive scaffold	1640:1678	Therefore, the Alg/Gel/mMWCNTs conductive scaffold has potential as a tissue engineering scaffold in nerve regeneration.					
36512950	0	78	theme	conductive	44:53	arg1	scaffold					55:62	a novel hybrid conductive scaffold	29:62	a novel hybrid conductive scaffold	29:62	Fabrication and detection of a novel hybrid conductive scaffold based on alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering.					
36512950	1	79	theme	excellent	301:309	arg1	conductivity					322:333	their excellent electrical conductivity	295:333	their excellent electrical conductivity	295:333	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	6	80	theme	increase	1139:1146	arg1	trend					1122:1126	a trend	1120:1126	a trend of gradual increase over culture time	1120:1164	In general, the mMWCNTs addition improved the hydrophilic, electrical conductivity and mechanical properties of the composite scaffold, and PC12 cells showed a trend of gradual increase over culture time.					
36512950	2	81	theme	alginate/gelatin	467:482	arg1	scaffolds					494:502	sodium alginate/gelatin (Alg/Gel) scaffolds	460:502	sodium alginate/gelatin (Alg/Gel) scaffolds	460:502	In this study, the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs) were introduced into sodium alginate/gelatin (Alg/Gel) scaffolds to optimize the function of the hybrid scaffolds.					
36512950	10	82	theme	engineering	1706:1716	arg1	scaffold					1718:1725	a tissue engineering scaffold	1697:1725	a tissue engineering scaffold in nerve regeneration	1697:1747	Therefore, the Alg/Gel/mMWCNTs conductive scaffold has potential as a tissue engineering scaffold in nerve regeneration.					
36512950	10	82	theme	engineering	1706:1716	arg1	potential					1684:1692	potential	1684:1692	potential	1684:1692	Therefore, the Alg/Gel/mMWCNTs conductive scaffold has potential as a tissue engineering scaffold in nerve regeneration.					
36512950	4	83	theme	magnetic	785:792	arg1	intensities					800:810	different magnetic field intensities	775:810	different magnetic field intensities	775:810	Following this, the physicochemical properties and biocompatibility of the hybrid scaffolds at different magnetic field intensities were evaluated.					
36512950	1	84	theme	nanomaterials	224:236	arg1	kinds					197:201	as kinds	194:201	as kinds of conductive carbon nanomaterials	194:236	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	1	84	theme	nanomaterials	224:236	arg1	nanotubes					176:184	Carbon nanotubes	169:184	Carbon nanotubes (CNTs)	169:191	Carbon nanotubes (CNTs), as kinds of conductive carbon nanomaterials, were widely applied in neural tissue engineering due to their excellent electrical conductivity and good biocompatibility.					
36512950	2	85	theme	multi-walled	399:410	arg1	mMWCNTs					430:436	mMWCNTs	430:436	mMWCNTs	430:436	In this study, the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs) were introduced into sodium alginate/gelatin (Alg/Gel) scaffolds to optimize the function of the hybrid scaffolds.					
36512950	2	85	theme	multi-walled	399:410	arg1	nanotubes					419:427	the carboxyl-modified multi-walled carbon nanotubes	377:427	the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs)	377:437	In this study, the carboxyl-modified multi-walled carbon nanotubes (mMWCNTs) were introduced into sodium alginate/gelatin (Alg/Gel) scaffolds to optimize the function of the hybrid scaffolds.					
36512950	8	86	theme	G1	1445:1446	arg1	S/cm					1414:1417	1.32 × 10-3 ± 2.1 × 10-4 S/cm	1389:1417	1.32 × 10-3 ± 2.1 × 10-4 S/cm	1389:1417	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	86	theme	G1	1445:1446	arg1	phase					1448:1452	the G1 phase	1441:1452	the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94%	1441:1488	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
36512950	8	87	dep	%	1524:1524	arg1	to					1526:1527	to	1526:1527	to	1526:1527	Briefly, the surface contact angle decreased from 74 ± 1° to 60 ± 3°, the electrical conductivity and compressive modulus increased to 1.32 × 10-3 ± 2.1 × 10-4 S/cm and 1.40 ± 0.076 Mpa, the G1 phase from 55.67 ± 1.86% to 59.77 ± 0.94% and the G2 phase from 10.32 ± 0.35% to 13.93 ± 1.26%，respectively.					
35677250	4	0	with	line	668:671	arg1	this					678:681	this	678:681	this	678:681	In line with this, the indigenous handmade paper making from the bark of Daphne papyracea, Wall.					
35677250	11	1	theme	commercial	1833:1842	arg1	processing					1844:1853	commercial processing	1833:1853	commercial processing	1833:1853	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	10	2	theme	wall	1509:1512	arg1	glycans					1514:1520	Non-cellulosic cell wall glycans	1489:1520	Non-cellulosic cell wall glycans	1489:1520	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	3	3	theme	resources	511:519	arg1	utilization					490:500	enormous utilization	481:500	enormous utilization of water resources	481:519	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	3	3	theme	resources	511:519	arg1	release					529:535	the release	525:535	the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures	525:662	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	10	4	theme	Non-cellulosic	1489:1502	arg1	glycans					1514:1520	Non-cellulosic cell wall glycans	1489:1520	Non-cellulosic cell wall glycans	1489:1520	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	13	5	theme	handmade	2120:2127	arg1	paper					2129:2133	the Himalayan Buddhist handmade paper	2097:2133	the Himalayan Buddhist handmade paper of Arunachal Pradesh	2097:2154	The presence of these polymers in the Himalayan Buddhist handmade paper of Arunachal Pradesh is correlated with its high tensile strength.					
35677250	9	6	theme	Plant	1386:1390	arg1	walls					1397:1401	Plant cell walls	1386:1401	Plant cell walls	1386:1401	Plant cell walls are primarily made up of cellulose, hemicellulose, lignin, pectin, and glycoproteins.					
35677250	12	7	theme	cell	1951:1954	arg1	xylan					1977:1981	xylan	1977:1981	xylan	1977:1981	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	12	7	theme	cell	1951:1954	arg1	rhamnogalacturonan-I					2006:2025	rhamnogalacturonan-I	2006:2025	rhamnogalacturonan-I	2006:2025	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	12	7	theme	cell	1951:1954	arg1	arabinogalactans					1984:1999	arabinogalactans	1984:1999	arabinogalactans	1984:1999	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	12	7	theme	cell	1951:1954	arg1	glycans					1961:1967	cell wall glycans	1951:1967	cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I	1951:2025	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	11	8	theme	glycosyl	1748:1755	arg1	analysis					1777:1784	glycosyl residue composition analysis	1748:1784	glycosyl residue composition analysis	1748:1784	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	2	9	theme	primary	270:276	arg1	material					282:289	the primary raw material	266:289	the primary raw material of the PPIs	266:301	While wood remains the primary raw material of the PPIs, the demand for paper has also grown alongside the expanding global population, leading to deforestation and ecological imbalance.					
35677250	12	10	theme	glycans	1961:1967	arg1	classes					1940:1946	major classes	1934:1946	major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I	1934:2025	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	6	11	theme	monoclonal	1062:1071	arg1	antibodies					1073:1082	glycan-directed monoclonal antibodies	1046:1082	glycan-directed monoclonal antibodies	1046:1082	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	0	12	theme	Pradesh	118:124	arg1	Region					98:103	Tawang Region	91:103	Tawang Region of Arunachal Pradesh	91:124	Glycome Profiling and Bioprospecting Potential of the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh.					
35677250	13	13	theme	Himalayan	2101:2109	arg1	paper					2129:2133	the Himalayan Buddhist handmade paper	2097:2133	the Himalayan Buddhist handmade paper of Arunachal Pradesh	2097:2154	The presence of these polymers in the Himalayan Buddhist handmade paper of Arunachal Pradesh is correlated with its high tensile strength.					
35677250	3	14	theme	untreated	561:569	arg1	sludge					571:576	untreated sludge	561:576	untreated sludge	561:576	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	1	15	theme	industries	183:192	arg1	one					164:166	one	164:166	one	164:166	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	1	15	theme	industries	183:192	arg1	industry					146:153	The paper and pulp industry	127:153	The paper and pulp industry (PPI)	127:159	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	1	15	theme	industries	183:192	arg1	industries					183:192	the largest industries	171:192	the largest industries that contribute to the growing economy of the world	171:244	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	10	16	theme	physical	1558:1565	arg1	crystallinity					1595:1607	crystallinity	1595:1607	crystallinity	1595:1607	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	10	16	theme	physical	1558:1565	arg1	properties					1567:1576	various physical properties	1550:1576	various physical properties such as density, crystallinity, and tensile strength of plant cell walls	1550:1649	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	10	16	theme	physical	1558:1565	arg1	density					1586:1592	density	1586:1592	density	1586:1592	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	10	16	theme	physical	1558:1565	arg1	strength					1622:1629	tensile strength	1614:1629	tensile strength	1614:1629	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	11	17	theme	composition	1765:1775	arg1	analysis					1777:1784	glycosyl residue composition analysis	1748:1784	glycosyl residue composition analysis	1748:1784	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	3	18	theme	wastes	550:555	arg1	utilization					490:500	enormous utilization	481:500	enormous utilization of water resources	481:519	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	3	18	theme	wastes	550:555	arg1	release					529:535	the release	525:535	the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures	525:662	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	14	19	theme	high-quality	2307:2318	arg1	paper					2320:2324	high-quality paper	2307:2324	high-quality paper	2307:2324	The results of this study imply that non-cellulosic cell wall glycans are required for the production of high-quality paper.					
35677250	11	20	theme	non-cellulosic	1686:1699	arg1	glycan					1711:1716	non-cellulosic cell wall glycan	1686:1716	non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis	1686:1784	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	5	21	theme	potential	838:846	arg1	alternative					848:858	a potential alternative	836:858	a potential alternative to non-wood fiber	836:876	ex G. Don by the Monpa tribe of Arunachal Pradesh, India is considered as a potential alternative to non-wood fiber.					
35677250	5	21	theme	potential	838:846	arg1	Don					768:770	ex G. Don	762:770	ex G. Don by the Monpa tribe of Arunachal Pradesh, India	762:817	ex G. Don by the Monpa tribe of Arunachal Pradesh, India is considered as a potential alternative to non-wood fiber.					
35677250	15	22	dep	marketing	2516:2524	arg1	aid					2526:2528	aid	2526:2528	aid	2526:2528	To summarize, immediate action is required to strengthen the centuries-old practice of handmade paper, which can be achieved through education, workshops, technical know-how, and effective marketing aid to entrepreneurs.					
35677250	7	23	theme	predictive	1168:1177	arg1	distribution					1179:1190	the maximum predictive distribution	1156:1190	the maximum predictive distribution of the plant toward the western parts of Arunachal Pradesh	1156:1249	The algorithms used for ecological and geographical modeling indicated the maximum predictive distribution of the plant toward the western parts of Arunachal Pradesh.					
35677250	15	24	theme	handmade	2414:2421	arg1	paper					2423:2427	handmade paper	2414:2427	handmade paper	2414:2427	To summarize, immediate action is required to strengthen the centuries-old practice of handmade paper, which can be achieved through education, workshops, technical know-how, and effective marketing aid to entrepreneurs.					
35677250	1	25	theme	pulp	141:144	arg1	PPI					156:158	PPI	156:158	PPI	156:158	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	1	25	theme	pulp	141:144	arg1	one					164:166	one	164:166	one	164:166	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	1	25	theme	pulp	141:144	arg1	industry					146:153	The paper and pulp industry	127:153	The paper and pulp industry (PPI)	127:159	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	1	25	theme	pulp	141:144	arg1	industries					183:192	the largest industries	171:192	the largest industries that contribute to the growing economy of the world	171:244	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	12	26	theme	D.	2043:2044	arg1	paper					2056:2060	D. papyracea paper	2043:2060	D. papyracea paper	2043:2060	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	13	27	attach	presence	2067:2074	arg2	polymers					2085:2092	these polymers	2079:2092	these polymers	2079:2092	The presence of these polymers in the Himalayan Buddhist handmade paper of Arunachal Pradesh is correlated with its high tensile strength.					
35677250	13	27	attach	presence	2067:2074	arg1	paper					2129:2133	the Himalayan Buddhist handmade paper	2097:2133	the Himalayan Buddhist handmade paper of Arunachal Pradesh	2097:2154	The presence of these polymers in the Himalayan Buddhist handmade paper of Arunachal Pradesh is correlated with its high tensile strength.					
35677250	0	28	theme	Paper	82:86	arg1	Profiling					8:16	Glycome Profiling	0:16	Glycome Profiling	0:16	Glycome Profiling and Bioprospecting Potential of the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh.					
35677250	0	28	theme	Paper	82:86	arg1	Potential					37:45	Bioprospecting Potential	22:45	Bioprospecting Potential	22:45	Glycome Profiling and Bioprospecting Potential of the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh.					
35677250	12	29	theme	ELISA-based	1878:1888	arg1	results					1908:1914	ELISA-based glycome profiling results	1878:1914	ELISA-based glycome profiling results	1878:1914	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	6	30	theme	distribution	912:923	arg1	modeling					925:932	the species distribution modeling	900:932	the species distribution modeling of D. papyracea	900:948	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	4	31	theme	indigenous	688:697	arg1	paper					708:712	the indigenous handmade paper	684:712	the indigenous handmade paper	684:712	In line with this, the indigenous handmade paper making from the bark of Daphne papyracea, Wall.					
35677250	11	32	theme	wall	1706:1709	arg1	glycan					1711:1716	non-cellulosic cell wall glycan	1686:1716	non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis	1686:1784	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	0	33	theme	Tawang	91:96	arg1	Region					98:103	Tawang Region	91:103	Tawang Region of Arunachal Pradesh	91:124	Glycome Profiling and Bioprospecting Potential of the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh.					
35677250	12	34	theme	profiling	1898:1906	arg1	results					1908:1914	ELISA-based glycome profiling results	1878:1914	ELISA-based glycome profiling results	1878:1914	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	8	35	dep	precipitation	1345:1357	arg1	the					1341:1343	the	1341:1343	the	1341:1343	It was also found that the suitable distribution of D. papyracea was largely affected by the precipitation and temperature variables.					
35677250	8	35	dep	precipitation	1345:1357	arg1	variables					1375:1383	variables	1375:1383	variables	1375:1383	It was also found that the suitable distribution of D. papyracea was largely affected by the precipitation and temperature variables.					
35677250	14	36	theme	wall	2259:2262	arg1	glycans					2264:2270	non-cellulosic cell wall glycans	2239:2270	non-cellulosic cell wall glycans	2239:2270	The results of this study imply that non-cellulosic cell wall glycans are required for the production of high-quality paper.					
35677250	11	37	theme	paper	1871:1875	arg1	composition					1817:1827	polymeric composition	1807:1827	polymeric composition	1807:1827	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	11	37	theme	paper	1871:1875	arg1	processing					1844:1853	commercial processing	1833:1853	commercial processing	1833:1853	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	15	38	theme	technical	2482:2490	arg1	know-how					2492:2499	technical know-how	2482:2499	technical know-how	2482:2499	To summarize, immediate action is required to strengthen the centuries-old practice of handmade paper, which can be achieved through education, workshops, technical know-how, and effective marketing aid to entrepreneurs.					
35677250	11	39	dep	composition	1817:1827	arg1	the					1803:1805	the	1803:1805	the	1803:1805	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	1	40	theme	paper	131:135	arg1	PPI					156:158	PPI	156:158	PPI	156:158	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	1	40	theme	paper	131:135	arg1	one					164:166	one	164:166	one	164:166	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	1	40	theme	paper	131:135	arg1	industry					146:153	The paper and pulp industry	127:153	The paper and pulp industry (PPI)	127:159	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	1	40	theme	paper	131:135	arg1	industries					183:192	the largest industries	171:192	the largest industries that contribute to the growing economy of the world	171:244	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	0	41	theme	Handmade	73:80	arg1	Paper					82:86	the Himalayan Buddhist Handmade Paper	50:86	the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh	50:124	Glycome Profiling and Bioprospecting Potential of the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh.					
35677250	8	42	theme	D.	1304:1305	arg1	papyracea					1307:1315	D. papyracea	1304:1315	D. papyracea	1304:1315	It was also found that the suitable distribution of D. papyracea was largely affected by the precipitation and temperature variables.					
35677250	14	43	theme	non-cellulosic	2239:2252	arg1	glycans					2264:2270	non-cellulosic cell wall glycans	2239:2270	non-cellulosic cell wall glycans	2239:2270	The results of this study imply that non-cellulosic cell wall glycans are required for the production of high-quality paper.					
35677250	11	44	theme	D.	1858:1859	arg1	paper					1871:1875	D. papyracea paper	1858:1875	D. papyracea paper	1858:1875	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	0	45	theme	Glycome	0:6	arg1	Profiling					8:16	Glycome Profiling	0:16	Glycome Profiling	0:16	Glycome Profiling and Bioprospecting Potential of the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh.					
35677250	1	46	theme	world	240:244	arg1	economy					225:231	the growing economy	213:231	the growing economy of the world	213:244	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	6	47	theme	paper	985:989	arg1	profiling					1004:1012	glycome profiling	996:1012	glycome profiling of the paper by plant cell wall	996:1044	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	6	47	theme	paper	985:989	arg1	modeling					925:932	the species distribution modeling	900:932	the species distribution modeling of D. papyracea	900:948	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	6	47	theme	paper	985:989	arg1	production					967:976	community-based production	951:976	community-based production of the paper	951:989	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	4	48	theme	handmade	699:706	arg1	paper					708:712	the indigenous handmade paper	684:712	the indigenous handmade paper	684:712	In line with this, the indigenous handmade paper making from the bark of Daphne papyracea, Wall.					
35677250	2	49	theme	global	364:369	arg1	population					371:380	the expanding global population	350:380	the expanding global population	350:380	While wood remains the primary raw material of the PPIs, the demand for paper has also grown alongside the expanding global population, leading to deforestation and ecological imbalance.					
35677250	5	50	theme	ex	762:763	arg1	alternative					848:858	a potential alternative	836:858	a potential alternative to non-wood fiber	836:876	ex G. Don by the Monpa tribe of Arunachal Pradesh, India is considered as a potential alternative to non-wood fiber.					
35677250	5	50	theme	ex	762:763	arg1	Don					768:770	ex G. Don	762:770	ex G. Don by the Monpa tribe of Arunachal Pradesh, India	762:817	ex G. Don by the Monpa tribe of Arunachal Pradesh, India is considered as a potential alternative to non-wood fiber.					
35677250	9	51	gly	glycoproteins	1474:1486	arg1	glycoproteins					1474:1486	glycoproteins	1474:1486	glycoproteins	1474:1486	Plant cell walls are primarily made up of cellulose, hemicellulose, lignin, pectin, and glycoproteins.					
35677250	3	52	theme	environment	611:621	arg1	quality					596:602	the quality	592:602	the quality of the environment	592:621	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	10	53	theme	tensile	1614:1620	arg1	strength					1622:1629	tensile strength	1614:1629	tensile strength	1614:1629	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	6	54	theme	community-based	951:965	arg1	production					967:976	community-based production	951:976	community-based production of the paper	951:989	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	0	55	theme	Buddhist	64:71	arg1	Paper					82:86	the Himalayan Buddhist Handmade Paper	50:86	the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh	50:124	Glycome Profiling and Bioprospecting Potential of the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh.					
35677250	3	56	theme	paper	445:449	arg1	production					451:460	Wood-based paper production	434:460	Wood-based paper production	434:460	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	13	57	theme	tensile	2184:2190	arg1	strength					2192:2199	its high tensile strength	2175:2199	its high tensile strength	2175:2199	The presence of these polymers in the Himalayan Buddhist handmade paper of Arunachal Pradesh is correlated with its high tensile strength.					
35677250	12	58	located	present	2032:2038	arg1	paper					2056:2060	D. papyracea paper	2043:2060	D. papyracea paper	2043:2060	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	12	58	located	present	2032:2038	arg2	classes					1940:1946	major classes	1934:1946	major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I	1934:2025	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	6	59	theme	cell	1036:1039	arg1	wall					1041:1044	plant cell wall	1030:1044	plant cell wall	1030:1044	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	11	60	theme	detailed	1665:1672	arg1	analysis					1674:1681	a detailed analysis	1663:1681	a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis	1663:1784	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	11	60	theme	detailed	1665:1672	arg1	important					1789:1797	important	1789:1797	important	1789:1797	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	10	61	theme	cell	1640:1643	arg1	walls					1645:1649	plant cell walls	1634:1649	plant cell walls	1634:1649	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	13	62	theme	Pradesh	2148:2154	arg1	paper					2129:2133	the Himalayan Buddhist handmade paper	2097:2133	the Himalayan Buddhist handmade paper of Arunachal Pradesh	2097:2154	The presence of these polymers in the Himalayan Buddhist handmade paper of Arunachal Pradesh is correlated with its high tensile strength.					
35677250	4	63	theme	papyracea	745:753	arg1	bark					730:733	the bark	726:733	the bark of Daphne papyracea, Wall	726:759	In line with this, the indigenous handmade paper making from the bark of Daphne papyracea, Wall.					
35677250	7	64	theme	plant	1199:1203	arg1	distribution					1179:1190	the maximum predictive distribution	1156:1190	the maximum predictive distribution of the plant toward the western parts of Arunachal Pradesh	1156:1249	The algorithms used for ecological and geographical modeling indicated the maximum predictive distribution of the plant toward the western parts of Arunachal Pradesh.					
35677250	13	65	from	presence	2067:2074	arg1	paper					2129:2133	the Himalayan Buddhist handmade paper	2097:2133	the Himalayan Buddhist handmade paper of Arunachal Pradesh	2097:2154	The presence of these polymers in the Himalayan Buddhist handmade paper of Arunachal Pradesh is correlated with its high tensile strength.					
35677250	1	66	theme	growing	217:223	arg1	economy					225:231	the growing economy	213:231	the growing economy of the world	213:244	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	15	67	theme	immediate	2341:2349	arg1	action					2351:2356	immediate action	2341:2356	immediate action	2341:2356	To summarize, immediate action is required to strengthen the centuries-old practice of handmade paper, which can be achieved through education, workshops, technical know-how, and effective marketing aid to entrepreneurs.					
35677250	5	68	dep	ex	762:763	arg1	G.					765:766	G.	765:766	G.	765:766	ex G. Don by the Monpa tribe of Arunachal Pradesh, India is considered as a potential alternative to non-wood fiber.					
35677250	3	69	theme	living	647:652	arg1	creatures					654:662	living creatures	647:662	living creatures	647:662	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	3	70	theme	water	505:509	arg1	resources					511:519	water resources	505:519	water resources	505:519	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	10	71	theme	cell	1504:1507	arg1	glycans					1514:1520	Non-cellulosic cell wall glycans	1489:1520	Non-cellulosic cell wall glycans	1489:1520	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	2	72	theme	PPIs	298:301	arg1	material					282:289	the primary raw material	266:289	the primary raw material of the PPIs	266:301	While wood remains the primary raw material of the PPIs, the demand for paper has also grown alongside the expanding global population, leading to deforestation and ecological imbalance.					
35677250	5	73	theme	Monpa	779:783	arg1	tribe					785:789	the Monpa tribe	775:789	the Monpa tribe of Arunachal Pradesh, India	775:817	ex G. Don by the Monpa tribe of Arunachal Pradesh, India is considered as a potential alternative to non-wood fiber.					
35677250	10	74	theme	various	1550:1556	arg1	crystallinity					1595:1607	crystallinity	1595:1607	crystallinity	1595:1607	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	10	74	theme	various	1550:1556	arg1	properties					1567:1576	various physical properties	1550:1576	various physical properties such as density, crystallinity, and tensile strength of plant cell walls	1550:1649	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	10	74	theme	various	1550:1556	arg1	density					1586:1592	density	1586:1592	density	1586:1592	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	10	74	theme	various	1550:1556	arg1	strength					1622:1629	tensile strength	1614:1629	tensile strength	1614:1629	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	2	75	theme	raw	278:280	arg1	material					282:289	the primary raw material	266:289	the primary raw material of the PPIs	266:301	While wood remains the primary raw material of the PPIs, the demand for paper has also grown alongside the expanding global population, leading to deforestation and ecological imbalance.					
35677250	9	76	theme	cell	1392:1395	arg1	walls					1397:1401	Plant cell walls	1386:1401	Plant cell walls	1386:1401	Plant cell walls are primarily made up of cellulose, hemicellulose, lignin, pectin, and glycoproteins.					
35677250	11	77	theme	residue	1757:1763	arg1	analysis					1777:1784	glycosyl residue composition analysis	1748:1784	glycosyl residue composition analysis	1748:1784	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	12	78	theme	wall	1956:1959	arg1	xylan					1977:1981	xylan	1977:1981	xylan	1977:1981	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	12	78	theme	wall	1956:1959	arg1	rhamnogalacturonan-I					2006:2025	rhamnogalacturonan-I	2006:2025	rhamnogalacturonan-I	2006:2025	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	12	78	theme	wall	1956:1959	arg1	arabinogalactans					1984:1999	arabinogalactans	1984:1999	arabinogalactans	1984:1999	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	12	78	theme	wall	1956:1959	arg1	glycans					1961:1967	cell wall glycans	1951:1967	cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I	1951:2025	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	6	79	theme	glycan-directed	1046:1060	arg1	antibodies					1073:1082	glycan-directed monoclonal antibodies	1046:1082	glycan-directed monoclonal antibodies	1046:1082	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	11	80	theme	polymeric	1807:1815	arg1	composition					1817:1827	polymeric composition	1807:1827	polymeric composition	1807:1827	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	1	81	theme	largest	175:181	arg1	industries					183:192	the largest industries	171:192	the largest industries that contribute to the growing economy of the world	171:244	The paper and pulp industry (PPI) is one of the largest industries that contribute to the growing economy of the world.					
35677250	7	82	theme	geographical	1124:1135	arg1	modeling					1137:1144	ecological and geographical modeling	1109:1144	ecological and geographical modeling	1109:1144	The algorithms used for ecological and geographical modeling indicated the maximum predictive distribution of the plant toward the western parts of Arunachal Pradesh.					
35677250	13	83	theme	Buddhist	2111:2118	arg1	paper					2129:2133	the Himalayan Buddhist handmade paper	2097:2133	the Himalayan Buddhist handmade paper of Arunachal Pradesh	2097:2154	The presence of these polymers in the Himalayan Buddhist handmade paper of Arunachal Pradesh is correlated with its high tensile strength.					
35677250	3	84	theme	different	540:548	arg1	wastes					550:555	different wastes	540:555	different wastes	540:555	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	12	85	theme	major	1934:1938	arg1	classes					1940:1946	major classes	1934:1946	major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I	1934:2025	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	12	86	attach	present	2032:2038	arg1	paper					2056:2060	D. papyracea paper	2043:2060	D. papyracea paper	2043:2060	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	12	86	attach	present	2032:2038	arg2	classes					1940:1946	major classes	1934:1946	major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I	1934:2025	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	12	87	theme	papyracea	2046:2054	arg1	paper					2056:2060	D. papyracea paper	2043:2060	D. papyracea paper	2043:2060	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	14	88	theme	paper	2320:2324	arg1	production					2293:2302	the production	2289:2302	the production of high-quality paper	2289:2324	The results of this study imply that non-cellulosic cell wall glycans are required for the production of high-quality paper.					
35677250	11	89	theme	cell	1701:1704	arg1	glycan					1711:1716	non-cellulosic cell wall glycan	1686:1716	non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis	1686:1784	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	7	90	theme	maximum	1160:1166	arg1	distribution					1179:1190	the maximum predictive distribution	1156:1190	the maximum predictive distribution of the plant toward the western parts of Arunachal Pradesh	1156:1249	The algorithms used for ecological and geographical modeling indicated the maximum predictive distribution of the plant toward the western parts of Arunachal Pradesh.					
35677250	13	91	theme	polymers	2085:2092	arg1	presence					2067:2074	The presence	2063:2074	The presence of these polymers in the Himalayan Buddhist handmade paper of Arunachal Pradesh	2063:2154	The presence of these polymers in the Himalayan Buddhist handmade paper of Arunachal Pradesh is correlated with its high tensile strength.					
35677250	3	92	theme	sludge	571:576	arg1	utilization					490:500	enormous utilization	481:500	enormous utilization of water resources	481:519	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	3	92	theme	sludge	571:576	arg1	release					529:535	the release	525:535	the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures	525:662	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	6	93	theme	D.	937:938	arg1	profiling					1004:1012	glycome profiling	996:1012	glycome profiling of the paper by plant cell wall	996:1044	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	6	93	theme	D.	937:938	arg1	modeling					925:932	the species distribution modeling	900:932	the species distribution modeling of D. papyracea	900:948	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	6	93	theme	D.	937:938	arg1	production					967:976	community-based production	951:976	community-based production of the paper	951:989	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	15	94	dep	summarize	2330:2338	arg1	To					2327:2328	To	2327:2328	To	2327:2328	To summarize, immediate action is required to strengthen the centuries-old practice of handmade paper, which can be achieved through education, workshops, technical know-how, and effective marketing aid to entrepreneurs.					
35677250	15	95	theme	paper	2423:2427	arg1	practice					2402:2409	the centuries-old practice	2384:2409	the centuries-old practice	2384:2409	To summarize, immediate action is required to strengthen the centuries-old practice of handmade paper, which can be achieved through education, workshops, technical know-how, and effective marketing aid to entrepreneurs.					
35677250	12	96	theme	glycome	1890:1896	arg1	results					1908:1914	ELISA-based glycome profiling results	1878:1914	ELISA-based glycome profiling results	1878:1914	ELISA-based glycome profiling results demonstrated that major classes of cell wall glycans such as xylan, arabinogalactans, and rhamnogalacturonan-I were present on D. papyracea paper.					
35677250	5	97	theme	India	813:817	arg1	tribe					785:789	the Monpa tribe	775:789	the Monpa tribe of Arunachal Pradesh, India	775:817	ex G. Don by the Monpa tribe of Arunachal Pradesh, India is considered as a potential alternative to non-wood fiber.					
35677250	6	98	dep	modeling	925:932	arg1	antibodies					1073:1082	glycan-directed monoclonal antibodies	1046:1082	glycan-directed monoclonal antibodies	1046:1082	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	11	99	theme	glycome	1726:1732	arg1	profiling					1734:1742	glycome profiling	1726:1742	glycome profiling	1726:1742	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	0	100	theme	Region	98:103	arg1	Paper					82:86	the Himalayan Buddhist Handmade Paper	50:86	the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh	50:124	Glycome Profiling and Bioprospecting Potential of the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh.					
35677250	6	101	theme	species	904:910	arg1	modeling					925:932	the species distribution modeling	900:932	the species distribution modeling of D. papyracea	900:948	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	6	102	dep	D.	937:938	arg1	papyracea					940:948	D. papyracea	937:948	D. papyracea	937:948	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	10	103	theme	plant	1634:1638	arg1	walls					1645:1649	plant cell walls	1634:1649	plant cell walls	1634:1649	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	7	104	theme	ecological	1109:1118	arg1	modeling					1137:1144	ecological and geographical modeling	1109:1144	ecological and geographical modeling	1109:1144	The algorithms used for ecological and geographical modeling indicated the maximum predictive distribution of the plant toward the western parts of Arunachal Pradesh.					
35677250	15	105	theme	centuries-old	2388:2400	arg1	practice					2402:2409	the centuries-old practice	2384:2409	the centuries-old practice	2384:2409	To summarize, immediate action is required to strengthen the centuries-old practice of handmade paper, which can be achieved through education, workshops, technical know-how, and effective marketing aid to entrepreneurs.					
35677250	6	106	theme	glycome	996:1002	arg1	profiling					1004:1012	glycome profiling	996:1012	glycome profiling of the paper by plant cell wall	996:1044	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	11	107	theme	glycan	1711:1716	arg1	analysis					1674:1681	a detailed analysis	1663:1681	a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis	1663:1784	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	11	107	theme	glycan	1711:1716	arg1	important					1789:1797	important	1789:1797	important	1789:1797	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	8	108	theme	suitable	1279:1286	arg1	distribution					1288:1299	the suitable distribution	1275:1299	the suitable distribution of D. papyracea	1275:1315	It was also found that the suitable distribution of D. papyracea was largely affected by the precipitation and temperature variables.					
35677250	14	109	theme	cell	2254:2257	arg1	glycans					2264:2270	non-cellulosic cell wall glycans	2239:2270	non-cellulosic cell wall glycans	2239:2270	The results of this study imply that non-cellulosic cell wall glycans are required for the production of high-quality paper.					
35677250	11	110	theme	papyracea	1861:1869	arg1	paper					1871:1875	D. papyracea paper	1858:1875	D. papyracea paper	1858:1875	Therefore, a detailed analysis of non-cellulosic cell wall glycan through glycome profiling and glycosyl residue composition analysis is important for the polymeric composition and commercial processing of D. papyracea paper.					
35677250	0	111	theme	Bioprospecting	22:35	arg1	Potential					37:45	Bioprospecting Potential	22:45	Bioprospecting Potential	22:45	Glycome Profiling and Bioprospecting Potential of the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh.					
35677250	5	112	theme	non-wood	863:870	arg1	fiber					872:876	non-wood fiber	863:876	non-wood fiber	863:876	ex G. Don by the Monpa tribe of Arunachal Pradesh, India is considered as a potential alternative to non-wood fiber.					
35677250	7	113	theme	Pradesh	1243:1249	arg1	parts					1224:1228	the western parts	1212:1228	the western parts of Arunachal Pradesh	1212:1249	The algorithms used for ecological and geographical modeling indicated the maximum predictive distribution of the plant toward the western parts of Arunachal Pradesh.					
35677250	8	114	theme	papyracea	1307:1315	arg1	distribution					1288:1299	the suitable distribution	1275:1299	the suitable distribution of D. papyracea	1275:1315	It was also found that the suitable distribution of D. papyracea was largely affected by the precipitation and temperature variables.					
35677250	14	115	theme	study	2222:2226	arg1	results					2206:2212	The results	2202:2212	The results of this study	2202:2226	The results of this study imply that non-cellulosic cell wall glycans are required for the production of high-quality paper.					
35677250	2	116	theme	expanding	354:362	arg1	population					371:380	the expanding global population	350:380	the expanding global population	350:380	While wood remains the primary raw material of the PPIs, the demand for paper has also grown alongside the expanding global population, leading to deforestation and ecological imbalance.					
35677250	2	117	theme	ecological	412:421	arg1	imbalance					423:431	deforestation and ecological imbalance	394:431	deforestation and ecological imbalance	394:431	While wood remains the primary raw material of the PPIs, the demand for paper has also grown alongside the expanding global population, leading to deforestation and ecological imbalance.					
35677250	0	118	theme	Himalayan	54:62	arg1	Paper					82:86	the Himalayan Buddhist Handmade Paper	50:86	the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh	50:124	Glycome Profiling and Bioprospecting Potential of the Himalayan Buddhist Handmade Paper of Tawang Region of Arunachal Pradesh.					
35677250	13	119	theme	high	2179:2182	arg1	strength					2192:2199	its high tensile strength	2175:2199	its high tensile strength	2175:2199	The presence of these polymers in the Himalayan Buddhist handmade paper of Arunachal Pradesh is correlated with its high tensile strength.					
35677250	7	120	theme	western	1216:1222	arg1	parts					1224:1228	the western parts	1212:1228	the western parts of Arunachal Pradesh	1212:1249	The algorithms used for ecological and geographical modeling indicated the maximum predictive distribution of the plant toward the western parts of Arunachal Pradesh.					
35677250	2	121	theme	deforestation	394:406	arg1	imbalance					423:431	deforestation and ecological imbalance	394:431	deforestation and ecological imbalance	394:431	While wood remains the primary raw material of the PPIs, the demand for paper has also grown alongside the expanding global population, leading to deforestation and ecological imbalance.					
35677250	10	122	theme	walls	1645:1649	arg1	crystallinity					1595:1607	crystallinity	1595:1607	crystallinity	1595:1607	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	10	122	theme	walls	1645:1649	arg1	density					1586:1592	density	1586:1592	density	1586:1592	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	10	122	theme	walls	1645:1649	arg1	strength					1622:1629	tensile strength	1614:1629	tensile strength	1614:1629	Non-cellulosic cell wall glycans contribute significantly to various physical properties such as density, crystallinity, and tensile strength of plant cell walls.					
35677250	6	123	theme	plant	1030:1034	arg1	wall					1041:1044	plant cell wall	1030:1044	plant cell wall	1030:1044	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	3	124	theme	Wood-based	434:443	arg1	production					451:460	Wood-based paper production	434:460	Wood-based paper production	434:460	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35677250	6	125	theme	paper	1021:1025	arg1	profiling					1004:1012	glycome profiling	996:1012	glycome profiling of the paper by plant cell wall	996:1044	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	6	125	theme	paper	1021:1025	arg1	modeling					925:932	the species distribution modeling	900:932	the species distribution modeling of D. papyracea	900:948	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	6	125	theme	paper	1021:1025	arg1	production					967:976	community-based production	951:976	community-based production of the paper	951:989	This study discusses the species distribution modeling of D. papyracea, community-based production of the paper, and glycome profiling of the paper by plant cell wall glycan-directed monoclonal antibodies.					
35677250	4	126	theme	Daphne	738:743	arg1	Wall					756:759	Wall	756:759	Wall	756:759	In line with this, the indigenous handmade paper making from the bark of Daphne papyracea, Wall.					
35677250	4	126	theme	Daphne	738:743	arg1	papyracea					745:753	Daphne papyracea	738:753	Daphne papyracea	738:753	In line with this, the indigenous handmade paper making from the bark of Daphne papyracea, Wall.					
35677250	3	127	theme	enormous	481:488	arg1	utilization					490:500	enormous utilization	481:500	enormous utilization of water resources	481:519	Wood-based paper production is associated with enormous utilization of water resources and the release of different wastes and untreated sludge that degrades the quality of the environment and makes it unsafe for living creatures.					
35163701	0	0	from	Production	175:184	arg1	Catalysis					56:64	the Unprecedented Catalysis	38:64	the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production	38:184	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	2	1	theme	TP	350:351	arg1	Stover					289:294	Corn Stover	284:294	Corn Stover (CS)	284:299	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	2	1	theme	TP	350:351	arg1	cartons					354:360	Tetra Pak (TP) cartons	339:360	Tetra Pak (TP) cartons	339:360	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	11	2	from	versatility	1767:1777	arg1	reuse					1786:1790	the reuse	1782:1790	the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals	1782:1987	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	11	3	theme	sustainable	1959:1969	arg1	goals					1983:1987	sustainable development goals	1959:1987	sustainable development goals	1959:1987	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	1	4	theme	Improper	187:194	arg1	disposal					218:225	Improper lignocellulosic waste disposal	187:225	Improper lignocellulosic waste disposal	187:225	Improper lignocellulosic waste disposal causes severe environmental pollution and health damage.					
35163701	6	5	dep	transform	1133:1141	arg1	infrared					1143:1150	infrared	1143:1150	transform infrared spectroscopy (FTIR)	1133:1170	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	2	6	theme	aseptic	320:326	arg1	Stover					289:294	Corn Stover	284:294	Corn Stover (CS)	284:299	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	2	6	theme	aseptic	320:326	arg1	packaging					328:336	agricultural, and aseptic packaging	302:336	agricultural, and aseptic packaging	302:336	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	9	7	theme	higher	1462:1467	arg1	mg/dl					1508:1512	328.8 and 996.8 mg/dl	1492:1512	328.8 and 996.8 mg/dl	1492:1512	NZ released a higher glucose concentration (328.8 and 996.8 mg/dl) than that of ZnCl2 (Z), which released 203.8 and 846.8 mg/dl from CS and TP, respectively.					
35163701	9	7	theme	higher	1462:1467	arg1	concentration					1477:1489	a higher glucose concentration	1460:1489	a higher glucose concentration (328.8 and 996.8 mg/dl)	1460:1513	NZ released a higher glucose concentration (328.8 and 996.8 mg/dl) than that of ZnCl2 (Z), which released 203.8 and 846.8 mg/dl from CS and TP, respectively.					
35163701	0	8	theme	Agricultural	112:123	arg1	Wastes					145:150	Sustainable Agricultural and Agro-Industrial Wastes	100:150	Sustainable Agricultural and Agro-Industrial Wastes	100:150	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	10	9	theme	ethanol	1660:1666	arg1	mg/dl					1651:1655	about 500 mg/dl	1641:1655	about 500 mg/dl	1641:1655	This work led to the production of about 500 mg/dl of ethanol, which is promising and a competitor to other studies.					
35163701	0	10	theme	Agro-Industrial	129:143	arg1	Wastes					145:150	Sustainable Agricultural and Agro-Industrial Wastes	100:150	Sustainable Agricultural and Agro-Industrial Wastes	100:150	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	3	11	theme	enzymatic	587:595	arg1	saccharification					597:612	enzymatic saccharification	587:612	enzymatic saccharification	587:612	In addition, attempts were made to enhance cellulose fractionation and improve enzymatic saccharification.					
35163701	1	12	theme	environmental	241:253	arg1	pollution					255:263	severe environmental pollution	234:263	severe environmental pollution	234:263	Improper lignocellulosic waste disposal causes severe environmental pollution and health damage.					
35163701	5	13	theme	pretreatment	880:891	arg1	strategy					893:900	the sequential pretreatment strategy	865:900	the sequential pretreatment strategy	865:900	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	6	14	theme	thermogravimetric	1177:1193	arg1	TGA					1205:1207	TGA	1205:1207	TGA	1205:1207	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	6	14	theme	thermogravimetric	1177:1193	arg1	analysis					1195:1202	thermogravimetric analysis	1177:1202	thermogravimetric analysis (TGA)	1177:1208	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	0	15	theme	Bioethanol	164:173	arg1	Production					175:184	Boosting Bioethanol Production	155:184	Boosting Bioethanol Production	155:184	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	5	16	from	catalyst	834:841	arg1	development					850:860	the development	846:860	the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis	846:939	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	6	17	theme	wastes	1012:1017	arg1	changes					959:965	Physico-chemical changes	942:965	Physico-chemical changes of the native and the pretreated sustainable wastes	942:1017	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	5	18	theme	hydrogen	765:772	arg1	Na2HPO4					785:791	Na2HPO4	785:791	Na2HPO4	785:791	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	5	18	theme	hydrogen	765:772	arg1	phosphate					774:782	disodium hydrogen phosphate	756:782	disodium hydrogen phosphate (Na2HPO4)	756:792	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	6	19	theme	pretreated	989:998	arg1	wastes					1012:1017	the pretreated sustainable wastes	985:1017	the pretreated sustainable wastes	985:1017	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	4	20	theme	bioethanol	692:701	arg1	production					703:712	bioethanol production	692:712	bioethanol production	692:712	In this regard, these two wastes were efficiently employed as substrates for bioethanol production.					
35163701	2	21	from	rich	428:431	arg1	materials					449:457	carbohydrate materials	436:457	carbohydrate materials	436:457	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	0	22	theme	Sequential	73:82	arg1	Pretreatment					84:95	the Sequential Pretreatment	69:95	the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production	69:184	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	11	23	theme	industrialization	1889:1905	arg1	areas					1858:1862	interaction areas	1846:1862	interaction areas of pollution prevention, industrialization, and clean energy production	1846:1934	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	8	24	theme	delignification	1351:1365	arg1	process					1367:1373	the delignification process	1347:1373	the delignification process	1347:1373	This pretreatment not only influences the delignification process, but also affects the functionalization of cellulose chemical structure.					
35163701	6	25	theme	electron	1070:1077	arg1	SEM					1091:1093	SEM	1091:1093	SEM	1091:1093	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	6	25	theme	electron	1070:1077	arg1	microscopy					1079:1088	scanning electron microscopy	1061:1088	scanning electron microscopy (SEM)	1061:1094	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	5	26	theme	noticeable	909:918	arg1	hydrolysis					930:939	the noticeable enzymatic hydrolysis	905:939	the noticeable enzymatic hydrolysis	905:939	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	11	27	theme	production	1925:1934	arg1	areas					1858:1862	interaction areas	1846:1862	interaction areas of pollution prevention, industrialization, and clean energy production	1846:1934	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	2	28	theme	wastes	412:417	arg1	Stover					289:294	Corn Stover	284:294	Corn Stover (CS)	284:299	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	2	28	theme	wastes	412:417	arg1	examples					388:395	two examples	384:395	two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products	384:505	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	0	29	theme	Combined	8:15	arg1	Na2HPO4					17:23	Combined Na2HPO4	8:23	Combined Na2HPO4	8:23	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	6	30	theme	compositional	1037:1049	arg1	analysis					1051:1058	compositional analysis	1037:1058	compositional analysis	1037:1058	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	11	31	theme	pollution	1867:1875	arg1	prevention					1877:1886	pollution prevention	1867:1886	pollution prevention	1867:1886	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	7	32	theme	optimized	1274:1282	arg1	pretreatment					1295:1306	the optimized sequential pretreatment	1270:1306	the optimized sequential pretreatment	1270:1306	These investigations showed major structural changes after the optimized sequential pretreatment.					
35163701	0	33	theme	Unprecedented	42:54	arg1	Catalysis					56:64	the Unprecedented Catalysis	38:64	the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production	38:184	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	5	34	theme	zinc	798:801	arg1	ZnCl2					813:817	ZnCl2	813:817	ZnCl2	813:817	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	5	34	theme	zinc	798:801	arg1	NZ					821:822	NZ	821:822	NZ	821:822	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	5	34	theme	zinc	798:801	arg1	chloride					803:810	zinc chloride	798:810	zinc chloride (ZnCl2) (NZ)	798:823	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	2	35	used	used	470:473	arg2	Stover					289:294	Corn Stover	284:294	Corn Stover (CS)	284:299	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	2	35	used	used	470:473	arg2	examples					388:395	two examples	384:395	two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products	384:505	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	8	36	theme	chemical	1428:1435	arg1	structure					1437:1445	cellulose chemical structure	1418:1445	cellulose chemical structure	1418:1445	This pretreatment not only influences the delignification process, but also affects the functionalization of cellulose chemical structure.					
35163701	2	37	theme	Tetra	339:343	arg1	Stover					289:294	Corn Stover	284:294	Corn Stover (CS)	284:299	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	2	37	theme	Tetra	339:343	arg1	cartons					354:360	Tetra Pak (TP) cartons	339:360	Tetra Pak (TP) cartons	339:360	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	1	38	theme	pollution	255:263	arg1	damage					276:281	severe environmental pollution and health damage	234:281	severe environmental pollution and health damage	234:281	Improper lignocellulosic waste disposal causes severe environmental pollution and health damage.					
35163701	7	39	theme	structural	1245:1254	arg1	changes					1256:1262	major structural changes	1239:1262	major structural changes	1239:1262	These investigations showed major structural changes after the optimized sequential pretreatment.					
35163701	11	40	theme	wastes	1828:1833	arg1	reuse					1786:1790	the reuse	1782:1790	the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals	1782:1987	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	5	41	theme	new	830:832	arg1	effect					746:751	the effect	742:751	the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ)	742:823	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	5	41	theme	new	830:832	arg1	catalyst					834:841	a new catalyst	828:841	a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis	828:939	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	1	42	theme	health	269:274	arg1	damage					276:281	severe environmental pollution and health damage	234:281	severe environmental pollution and health damage	234:281	Improper lignocellulosic waste disposal causes severe environmental pollution and health damage.					
35163701	2	43	theme	agricultural	302:313	arg1	Stover					289:294	Corn Stover	284:294	Corn Stover (CS)	284:299	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	2	43	theme	agricultural	302:313	arg1	packaging					328:336	agricultural, and aseptic packaging	302:336	agricultural, and aseptic packaging	302:336	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	1	44	theme	lignocellulosic	196:210	arg1	disposal					218:225	Improper lignocellulosic waste disposal	187:225	Improper lignocellulosic waste disposal	187:225	Improper lignocellulosic waste disposal causes severe environmental pollution and health damage.					
35163701	6	45	dep	Fourier	1125:1131	arg1	transform					1133:1141	transform	1133:1141	transform infrared spectroscopy (FTIR)	1133:1170	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	11	46	theme	development	1971:1981	arg1	goals					1983:1987	sustainable development goals	1959:1987	sustainable development goals	1959:1987	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	9	47	theme	glucose	1469:1475	arg1	mg/dl					1508:1512	328.8 and 996.8 mg/dl	1492:1512	328.8 and 996.8 mg/dl	1492:1512	NZ released a higher glucose concentration (328.8 and 996.8 mg/dl) than that of ZnCl2 (Z), which released 203.8 and 846.8 mg/dl from CS and TP, respectively.					
35163701	9	47	theme	glucose	1469:1475	arg1	concentration					1477:1489	a higher glucose concentration	1460:1489	a higher glucose concentration (328.8 and 996.8 mg/dl)	1460:1513	NZ released a higher glucose concentration (328.8 and 996.8 mg/dl) than that of ZnCl2 (Z), which released 203.8 and 846.8 mg/dl from CS and TP, respectively.					
35163701	3	48	theme	cellulose	551:559	arg1	fractionation					561:573	cellulose fractionation	551:573	cellulose fractionation	551:573	In addition, attempts were made to enhance cellulose fractionation and improve enzymatic saccharification.					
35163701	6	49	theme	Physico-chemical	942:957	arg1	changes					959:965	Physico-chemical changes	942:965	Physico-chemical changes of the native and the pretreated sustainable wastes	942:1017	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	2	50	theme	Pak	345:347	arg1	Stover					289:294	Corn Stover	284:294	Corn Stover (CS)	284:299	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	2	50	theme	Pak	345:347	arg1	cartons					354:360	Tetra Pak (TP) cartons	339:360	Tetra Pak (TP) cartons	339:360	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	0	51	theme	Wastes	145:150	arg1	Pretreatment					84:95	the Sequential Pretreatment	69:95	the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production	69:184	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	11	52	theme	clean	1912:1916	arg1	production					1925:1934	clean energy production	1912:1934	clean energy production	1912:1934	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	1	53	theme	waste	212:216	arg1	disposal					218:225	Improper lignocellulosic waste disposal	187:225	Improper lignocellulosic waste disposal	187:225	Improper lignocellulosic waste disposal causes severe environmental pollution and health damage.					
35163701	1	54	theme	severe	234:239	arg1	pollution					255:263	severe environmental pollution	234:263	severe environmental pollution	234:263	Improper lignocellulosic waste disposal causes severe environmental pollution and health damage.					
35163701	0	55	from	Catalysis	56:64	arg1	Production					175:184	Boosting Bioethanol Production	155:184	Boosting Bioethanol Production	155:184	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	0	56	theme	Boosting	155:162	arg1	Production					175:184	Boosting Bioethanol Production	155:184	Boosting Bioethanol Production	155:184	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	5	57	theme	sequential	869:878	arg1	strategy					893:900	the sequential pretreatment strategy	865:900	the sequential pretreatment strategy	865:900	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	0	58	from	Role	0:3	arg1	Catalysis					56:64	the Unprecedented Catalysis	38:64	the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production	38:184	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	5	59	theme	strategy	893:900	arg1	development					850:860	the development	846:860	the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis	846:939	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	10	60	theme	other	1708:1712	arg1	studies					1714:1720	other studies	1708:1720	other studies	1708:1720	This work led to the production of about 500 mg/dl of ethanol, which is promising and a competitor to other studies.					
35163701	0	61	from	Pretreatment	84:95	arg1	Production					175:184	Boosting Bioethanol Production	155:184	Boosting Bioethanol Production	155:184	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	5	62	theme	disodium	756:763	arg1	Na2HPO4					785:791	Na2HPO4	785:791	Na2HPO4	785:791	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	5	62	theme	disodium	756:763	arg1	phosphate					774:782	disodium hydrogen phosphate	756:782	disodium hydrogen phosphate (Na2HPO4)	756:792	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	2	63	theme	Corn	284:287	arg1	packaging					328:336	agricultural, and aseptic packaging	302:336	agricultural, and aseptic packaging	302:336	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	2	63	theme	Corn	284:287	arg1	CS					297:298	CS	297:298	CS	297:298	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	2	63	theme	Corn	284:287	arg1	cartons					354:360	Tetra Pak (TP) cartons	339:360	Tetra Pak (TP) cartons	339:360	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	2	63	theme	Corn	284:287	arg1	examples					388:395	two examples	384:395	two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products	384:505	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	2	63	theme	Corn	284:287	arg1	agro-industrial					363:377	agro-industrial	363:377	agro-industrial	363:377	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	2	63	theme	Corn	284:287	arg1	Stover					289:294	Corn Stover	284:294	Corn Stover (CS)	284:299	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	6	64	theme	sustainable	1000:1010	arg1	wastes					1012:1017	the pretreated sustainable wastes	985:1017	the pretreated sustainable wastes	985:1017	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	5	65	theme	phosphate	774:782	arg1	effect					746:751	the effect	742:751	the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ)	742:823	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	5	65	theme	phosphate	774:782	arg1	catalyst					834:841	a new catalyst	828:841	a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis	828:939	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	11	66	theme	agro-industrial	1812:1826	arg1	wastes					1828:1833	agricultural and agro-industrial wastes	1795:1833	agricultural and agro-industrial wastes	1795:1833	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	0	67	theme	Pretreatment	84:95	arg1	Catalysis					56:64	the Unprecedented Catalysis	38:64	the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production	38:184	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	11	68	theme	goals	1983:1987	arg1	keys					1951:1954	the keys	1947:1954	the keys of sustainable development goals	1947:1987	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	6	69	theme	native	974:979	arg1	changes					959:965	Physico-chemical changes	942:965	Physico-chemical changes of the native and the pretreated sustainable wastes	942:1017	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	2	70	from	materials	449:457	arg1	rich					428:431	rich	428:431	rich	428:431	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	6	71	theme	X-ray	1097:1101	arg1	diffractometry					1103:1116	X-ray diffractometry	1097:1116	X-ray diffractometry (XRD)	1097:1122	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	6	71	theme	X-ray	1097:1101	arg1	XRD					1119:1121	XRD	1119:1121	XRD	1119:1121	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	0	72	theme	Sustainable	100:110	arg1	Wastes					145:150	Sustainable Agricultural and Agro-Industrial Wastes	100:150	Sustainable Agricultural and Agro-Industrial Wastes	100:150	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	5	73	from	development	850:860	arg1	hydrolysis					930:939	the noticeable enzymatic hydrolysis	905:939	the noticeable enzymatic hydrolysis	905:939	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	2	74	theme	valuable	486:493	arg1	by-products					495:505	valuable by-products	486:505	valuable by-products	486:505	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	5	75	theme	chloride	803:810	arg1	effect					746:751	the effect	742:751	the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ)	742:823	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	5	75	theme	chloride	803:810	arg1	catalyst					834:841	a new catalyst	828:841	a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis	828:939	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	6	76	theme	scanning	1061:1068	arg1	SEM					1091:1093	SEM	1091:1093	SEM	1091:1093	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	6	76	theme	scanning	1061:1068	arg1	microscopy					1079:1088	scanning electron microscopy	1061:1088	scanning electron microscopy (SEM)	1061:1094	Physico-chemical changes of the native and the pretreated sustainable wastes were evaluated by compositional analysis, scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).					
35163701	11	77	theme	interaction	1846:1856	arg1	areas					1858:1862	interaction areas	1846:1862	interaction areas of pollution prevention, industrialization, and clean energy production	1846:1934	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	0	78	theme	Na2HPO4	17:23	arg1	Role					0:3	Role	0:3	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.	0:185	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	5	79	theme	enzymatic	920:928	arg1	hydrolysis					930:939	the noticeable enzymatic hydrolysis	905:939	the noticeable enzymatic hydrolysis	905:939	This research demonstrates the effect of disodium hydrogen phosphate (Na2HPO4) and zinc chloride (ZnCl2) (NZ) as a new catalyst on the development of the sequential pretreatment strategy in the noticeable enzymatic hydrolysis.					
35163701	7	80	theme	sequential	1284:1293	arg1	pretreatment					1295:1306	the optimized sequential pretreatment	1270:1306	the optimized sequential pretreatment	1270:1306	These investigations showed major structural changes after the optimized sequential pretreatment.					
35163701	2	81	theme	sustainable	400:410	arg1	wastes					412:417	sustainable wastes	400:417	sustainable wastes	400:417	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	0	82	theme	ZnCl2	29:33	arg1	Role					0:3	Role	0:3	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.	0:185	Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production.					
35163701	11	83	theme	prevention	1877:1886	arg1	areas					1858:1862	interaction areas	1846:1862	interaction areas of pollution prevention, industrialization, and clean energy production	1846:1934	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	2	84	theme	carbohydrate	436:447	arg1	materials					449:457	carbohydrate materials	436:457	carbohydrate materials	436:457	Corn Stover (CS), agricultural, and aseptic packaging, Tetra Pak (TP) cartons, agro-industrial, are two examples of sustainable wastes that are rich in carbohydrate materials and may be used to produce valuable by-products.					
35163701	10	85	theme	mg/dl	1651:1655	arg1	competitor					1694:1703	a competitor	1692:1703	a competitor to other studies	1692:1720	This work led to the production of about 500 mg/dl of ethanol, which is promising and a competitor to other studies.					
35163701	10	85	theme	mg/dl	1651:1655	arg1	production					1627:1636	the production	1623:1636	the production of about 500 mg/dl of ethanol, which is promising	1623:1686	This work led to the production of about 500 mg/dl of ethanol, which is promising and a competitor to other studies.					
35163701	11	86	theme	agricultural	1795:1806	arg1	wastes					1828:1833	agricultural and agro-industrial wastes	1795:1833	agricultural and agro-industrial wastes	1795:1833	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	11	87	theme	energy	1918:1923	arg1	production					1925:1934	clean energy production	1912:1934	clean energy production	1912:1934	These findings contribute to increasing the versatility in the reuse of agricultural and agro-industrial wastes to promote interaction areas of pollution prevention, industrialization, and clean energy production, to attain the keys of sustainable development goals.					
35163701	7	88	theme	major	1239:1243	arg1	changes					1256:1262	major structural changes	1239:1262	major structural changes	1239:1262	These investigations showed major structural changes after the optimized sequential pretreatment.					
35163701	8	89	theme	cellulose	1418:1426	arg1	structure					1437:1445	cellulose chemical structure	1418:1445	cellulose chemical structure	1418:1445	This pretreatment not only influences the delignification process, but also affects the functionalization of cellulose chemical structure.					
35163701	8	90	theme	structure	1437:1445	arg1	functionalization					1397:1413	the functionalization	1393:1413	the functionalization of cellulose chemical structure	1393:1445	This pretreatment not only influences the delignification process, but also affects the functionalization of cellulose chemical structure.					
35858665	3	0	theme	CC	709:710	arg1	mice					713:716	male and female Collaborative Cross (CC) mice	672:716	male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks	672:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	3	1	theme	Collaborative	688:700	arg1	mice					713:716	male and female Collaborative Cross (CC) mice	672:716	male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks	672:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	6	2	theme	FADS1	1368:1372	arg1	enzymes					1384:1390	FADS1 and FADS2 enzymes	1368:1390	FADS1 and FADS2 enzymes	1368:1390	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	1	3	theme	chronic	242:248	arg1	disease					256:262	chronic liver disease	242:262	chronic liver disease	242:262	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	6	4	theme	acid	1175:1178	arg1	composition					1180:1190	fatty acid composition	1169:1190	fatty acid composition	1169:1190	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	1	5	theme	disease	256:262	arg1	forms					233:237	the most common forms	217:237	the most common forms of chronic liver disease	217:262	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	6	6	theme	SCD1	1295:1298	arg1	enzyme					1300:1305	MUFA generating SCD1 enzyme	1279:1305	MUFA generating SCD1 enzyme	1279:1305	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	8	7	theme	diagnostic	1784:1793	arg1	marker					1795:1800	a diagnostic marker	1782:1800	a diagnostic marker for NAFLD severity	1782:1819	These results demonstrate that different models of NAFLD are characterized by similar changes in the esterified fatty acid composition and that alterations in PUFA/MUFA ratios may serve as a diagnostic marker for NAFLD severity.					
35858665	8	7	theme	diagnostic	1784:1793	arg1	alterations					1737:1747	alterations	1737:1747	alterations in PUFA/MUFA ratios	1737:1767	These results demonstrate that different models of NAFLD are characterized by similar changes in the esterified fatty acid composition and that alterations in PUFA/MUFA ratios may serve as a diagnostic marker for NAFLD severity.					
35858665	3	8	theme	liquid	531:536	arg1	chromatography					538:551	ultra-high performance liquid chromatography	508:551	ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry	508:602	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	8	9	theme	different	1624:1632	arg1	models					1634:1639	different models	1624:1639	different models of NAFLD	1624:1648	These results demonstrate that different models of NAFLD are characterized by similar changes in the esterified fatty acid composition and that alterations in PUFA/MUFA ratios may serve as a diagnostic marker for NAFLD severity.					
35858665	3	10	theme	sucrose	738:744	arg1	diet					754:757	a high fat/high sucrose (HF/HS) diet	722:757	a high fat/high sucrose (HF/HS) diet for 12 weeks	722:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	3	11	theme	mass	586:589	arg1	spectrometry					591:602	electrospray tandem mass spectrometry	566:602	electrospray tandem mass spectrometry	566:602	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	5	12	located	observed	1019:1026	arg2	changes					989:995	Similar changes	981:995	Similar changes in MUFA and PUFA	981:1012	Similar changes in MUFA and PUFA were observed in a choline- and folate-deficient (CFD) mouse model of NAFLD, as well as in hepatocytes treated in vitro with free fatty acids.					
35858665	5	12	located	observed	1019:1026	arg1	model					1075:1079	a choline- and folate-deficient (CFD) mouse model	1031:1079	a choline- and folate-deficient (CFD) mouse model of NAFLD	1031:1088	Similar changes in MUFA and PUFA were observed in a choline- and folate-deficient (CFD) mouse model of NAFLD, as well as in hepatocytes treated in vitro with free fatty acids.					
35858665	3	13	theme	high	724:727	arg1	diet					754:757	a high fat/high sucrose (HF/HS) diet	722:757	a high fat/high sucrose (HF/HS) diet for 12 weeks	722:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	3	14	theme	electrospray	566:577	arg1	spectrometry					591:602	electrospray tandem mass spectrometry	566:602	electrospray tandem mass spectrometry	566:602	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	7	15	theme	hepatic	1540:1546	arg1	injury					1548:1553	hepatic injury	1540:1553	hepatic injury in CFD diet-fed mouse model of NAFLD	1540:1590	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	4	16	theme	monounsaturated	893:907	arg1	MUFA					922:925	MUFA	922:925	MUFA	922:925	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	4	16	theme	monounsaturated	893:907	arg1	acids					915:919	monounsaturated fatty acids	893:919	monounsaturated fatty acids (MUFA)	893:926	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	0	17	theme	Collaborative	141:153	arg1	mice					161:164	genetically diverse Collaborative Cross mice	121:164	genetically diverse Collaborative Cross mice	121:164	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	4	18	from	increases	880:888	arg1	acids					967:971	polyunsaturated fatty acids	945:971	polyunsaturated fatty acids (PUFA)	945:978	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	4	18	from	increases	880:888	arg1	MUFA					922:925	MUFA	922:925	MUFA	922:925	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	4	18	from	increases	880:888	arg1	acids					915:919	monounsaturated fatty acids	893:919	monounsaturated fatty acids (MUFA)	893:926	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	4	18	from	increases	880:888	arg1	PUFA					974:977	PUFA	974:977	PUFA	974:977	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	3	19	theme	HF/HS	747:751	arg1	diet					754:757	a high fat/high sucrose (HF/HS) diet	722:757	a high fat/high sucrose (HF/HS) diet for 12 weeks	722:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	0	20	from	disease	110:116	arg1	mice					161:164	genetically diverse Collaborative Cross mice	121:164	genetically diverse Collaborative Cross mice	121:164	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	3	21	theme	acid	638:641	arg1	composition					643:653	the esterified fatty acid composition	617:653	the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks	617:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	4	22	from	Changes	773:779	arg1	composition					790:800	lipid composition	784:800	lipid composition	784:800	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	2	23	theme	hepatic	480:486	arg1	lipotoxicity					488:499	hepatic lipotoxicity	480:499	hepatic lipotoxicity	480:499	Recent studies have indicated that in addition to the total lipid quantities, changes in lipid composition are a determining factor in hepatic lipotoxicity.					
35858665	6	24	theme	enzyme	1300:1305	arg1	activity					1267:1274	the estimated activity	1253:1274	the estimated activity of MUFA generating SCD1 enzyme	1253:1305	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	1	25	theme	lipid	315:319	arg1	species					321:327	lipid species	315:327	lipid species	315:327	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	3	26	theme	esterified	621:630	arg1	composition					643:653	the esterified fatty acid composition	617:653	the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks	617:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	8	27	theme	fatty	1705:1709	arg1	composition					1716:1726	the esterified fatty acid composition	1690:1726	the esterified fatty acid composition	1690:1726	These results demonstrate that different models of NAFLD are characterized by similar changes in the esterified fatty acid composition and that alterations in PUFA/MUFA ratios may serve as a diagnostic marker for NAFLD severity.					
35858665	8	28	from	changes	1679:1685	arg1	composition					1716:1726	the esterified fatty acid composition	1690:1726	the esterified fatty acid composition	1690:1726	These results demonstrate that different models of NAFLD are characterized by similar changes in the esterified fatty acid composition and that alterations in PUFA/MUFA ratios may serve as a diagnostic marker for NAFLD severity.					
35858665	0	29	theme	diet-induced	72:83	arg1	disease					110:116	diet-induced nonalcoholic fatty liver disease	72:116	diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice	72:164	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	1	30	theme	fatty	181:185	arg1	NAFLD					202:206	NAFLD	202:206	NAFLD	202:206	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	1	30	theme	fatty	181:185	arg1	one					210:212	one	210:212	one	210:212	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	1	30	theme	fatty	181:185	arg1	disease					193:199	Non-alcoholic fatty liver disease	167:199	Non-alcoholic fatty liver disease (NAFLD)	167:207	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	1	30	theme	fatty	181:185	arg1	forms					233:237	the most common forms	217:237	the most common forms of chronic liver disease	217:262	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	2	31	from	changes	423:429	arg1	composition					440:450	lipid composition	434:450	lipid composition	434:450	Recent studies have indicated that in addition to the total lipid quantities, changes in lipid composition are a determining factor in hepatic lipotoxicity.					
35858665	0	32	theme	fatty	98:102	arg1	disease					110:116	diet-induced nonalcoholic fatty liver disease	72:116	diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice	72:164	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	7	33	from	markers	1529:1535	arg1	model					1577:1581	CFD diet-fed mouse model	1558:1581	CFD diet-fed mouse model of NAFLD	1558:1590	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	7	34	theme	female	1472:1477	arg1	mice					1482:1485	male and female CC mice	1463:1485	male and female CC mice fed the HF/HS diet	1463:1504	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	6	35	theme	estimated	1257:1265	arg1	activity					1267:1274	the estimated activity	1253:1274	the estimated activity of MUFA generating SCD1 enzyme	1253:1305	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	2	36	dep	quantities	411:420	arg1	addition					383:390	addition	383:390	addition	383:390	Recent studies have indicated that in addition to the total lipid quantities, changes in lipid composition are a determining factor in hepatic lipotoxicity.					
35858665	0	37	theme	Lipidomic	0:8	arg1	profiling					10:18	Lipidomic profiling	0:18	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.	0:165	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	6	38	from	increase	1241:1248	arg1	activity					1340:1347	the activity	1336:1347	the activity of PUFA generating FADS1 and FADS2 enzymes	1336:1390	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	6	38	from	increase	1241:1248	arg1	activity					1267:1274	the estimated activity	1253:1274	the estimated activity of MUFA generating SCD1 enzyme	1253:1305	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	7	39	theme	mouse	1571:1575	arg1	model					1577:1581	CFD diet-fed mouse model	1558:1581	CFD diet-fed mouse model of NAFLD	1558:1590	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	6	40	theme	generating	1284:1293	arg1	enzyme					1300:1305	MUFA generating SCD1 enzyme	1279:1305	MUFA generating SCD1 enzyme	1279:1305	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	2	41	theme	lipid	405:409	arg1	quantities					411:420	the total lipid quantities	395:420	the total lipid quantities	395:420	Recent studies have indicated that in addition to the total lipid quantities, changes in lipid composition are a determining factor in hepatic lipotoxicity.					
35858665	7	42	theme	CFD	1558:1560	arg1	model					1577:1581	CFD diet-fed mouse model	1558:1581	CFD diet-fed mouse model of NAFLD	1558:1590	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	4	43	theme	fatty	961:965	arg1	acids					967:971	polyunsaturated fatty acids	945:971	polyunsaturated fatty acids (PUFA)	945:978	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	4	43	theme	fatty	961:965	arg1	PUFA					974:977	PUFA	974:977	PUFA	974:977	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	4	44	theme	lipid	784:788	arg1	composition					790:800	lipid composition	784:800	lipid composition	784:800	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	0	45	theme	hepatic	27:33	arg1	composition					57:67	the hepatic esterified fatty acid composition	23:67	the hepatic esterified fatty acid composition	23:67	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	7	46	theme	lipid	1441:1445	arg1	accumulation					1447:1458	lipid accumulation	1441:1458	lipid accumulation in male and female CC mice fed the HF/HS diet	1441:1504	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	0	47	theme	composition	57:67	arg1	profiling					10:18	Lipidomic profiling	0:18	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.	0:165	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	0	48	theme	fatty	46:50	arg1	composition					57:67	the hepatic esterified fatty acid composition	23:67	the hepatic esterified fatty acid composition	23:67	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	5	49	theme	NAFLD	1084:1088	arg1	model					1075:1079	a choline- and folate-deficient (CFD) mouse model	1031:1079	a choline- and folate-deficient (CFD) mouse model of NAFLD	1031:1088	Similar changes in MUFA and PUFA were observed in a choline- and folate-deficient (CFD) mouse model of NAFLD, as well as in hepatocytes treated in vitro with free fatty acids.					
35858665	5	50	theme	Similar	981:987	arg1	changes					989:995	Similar changes	981:995	Similar changes in MUFA and PUFA	981:1012	Similar changes in MUFA and PUFA were observed in a choline- and folate-deficient (CFD) mouse model of NAFLD, as well as in hepatocytes treated in vitro with free fatty acids.					
35858665	5	51	dep	choline-	1033:1040	arg1	CFD					1064:1066	CFD	1064:1066	CFD	1064:1066	Similar changes in MUFA and PUFA were observed in a choline- and folate-deficient (CFD) mouse model of NAFLD, as well as in hepatocytes treated in vitro with free fatty acids.					
35858665	3	52	theme	female	681:686	arg1	mice					713:716	male and female Collaborative Cross (CC) mice	672:716	male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks	672:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	3	53	theme	male	672:675	arg1	mice					713:716	male and female Collaborative Cross (CC) mice	672:716	male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks	672:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	5	54	theme	fatty	1144:1148	arg1	acids					1150:1154	free fatty acids	1139:1154	free fatty acids	1139:1154	Similar changes in MUFA and PUFA were observed in a choline- and folate-deficient (CFD) mouse model of NAFLD, as well as in hepatocytes treated in vitro with free fatty acids.					
35858665	6	55	theme	estimated	1314:1322	arg1	decrease					1324:1331	an estimated decrease	1311:1331	an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes	1311:1390	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	7	56	theme	PUFA/MUFA	1393:1401	arg1	ratios					1403:1408	PUFA/MUFA ratios	1393:1408	PUFA/MUFA ratios	1393:1408	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	4	57	located	found	807:811	arg1	strains					820:826	all strains	816:826	all strains	816:826	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	4	57	located	found	807:811	arg2	Changes					773:779	Changes	773:779	Changes in lipid composition	773:800	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	5	58	from	changes	989:995	arg1	MUFA					1000:1003	MUFA	1000:1003	MUFA	1000:1003	Similar changes in MUFA and PUFA were observed in a choline- and folate-deficient (CFD) mouse model of NAFLD, as well as in hepatocytes treated in vitro with free fatty acids.					
35858665	5	58	from	changes	989:995	arg1	PUFA					1009:1012	PUFA	1009:1012	PUFA	1009:1012	Similar changes in MUFA and PUFA were observed in a choline- and folate-deficient (CFD) mouse model of NAFLD, as well as in hepatocytes treated in vitro with free fatty acids.					
35858665	1	59	from	accumulation	299:310	arg1	hepatocytes					332:342	hepatocytes	332:342	hepatocytes	332:342	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	2	60	theme	Recent	345:350	arg1	studies					352:358	Recent studies	345:358	Recent studies	345:358	Recent studies have indicated that in addition to the total lipid quantities, changes in lipid composition are a determining factor in hepatic lipotoxicity.					
35858665	3	61	from	composition	643:653	arg1	strains					661:667	24 strains	658:667	24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks	658:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	3	62	theme	Cross	702:706	arg1	mice					713:716	male and female Collaborative Cross (CC) mice	672:716	male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks	672:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	6	63	theme	composition	1180:1190	arg1	Analysis					1157:1164	Analysis	1157:1164	Analysis of fatty acid composition	1157:1190	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	1	64	theme	liver	250:254	arg1	disease					256:262	chronic liver disease	242:262	chronic liver disease	242:262	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	7	65	from	accumulation	1447:1458	arg1	mice					1482:1485	male and female CC mice	1463:1485	male and female CC mice fed the HF/HS diet	1463:1504	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	4	66	theme	HF/HS	838:842	arg1	diet					844:847	the HF/HS diet	834:847	the HF/HS diet	834:847	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	6	67	theme	fatty	1169:1173	arg1	composition					1180:1190	fatty acid composition	1169:1190	fatty acid composition	1169:1190	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	3	68	theme	performance	519:529	arg1	chromatography					538:551	ultra-high performance liquid chromatography	508:551	ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry	508:602	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	1	69	theme	liver	187:191	arg1	NAFLD					202:206	NAFLD	202:206	NAFLD	202:206	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	1	69	theme	liver	187:191	arg1	one					210:212	one	210:212	one	210:212	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	1	69	theme	liver	187:191	arg1	disease					193:199	Non-alcoholic fatty liver disease	167:199	Non-alcoholic fatty liver disease (NAFLD)	167:207	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	1	69	theme	liver	187:191	arg1	forms					233:237	the most common forms	217:237	the most common forms of chronic liver disease	217:262	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	3	70	theme	mice	713:716	arg1	strains					661:667	24 strains	658:667	24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks	658:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	8	71	theme	NAFLD	1806:1810	arg1	severity					1812:1819	NAFLD severity	1806:1819	NAFLD severity	1806:1819	These results demonstrate that different models of NAFLD are characterized by similar changes in the esterified fatty acid composition and that alterations in PUFA/MUFA ratios may serve as a diagnostic marker for NAFLD severity.					
35858665	3	72	theme	tandem	579:584	arg1	spectrometry					591:602	electrospray tandem mass spectrometry	566:602	electrospray tandem mass spectrometry	566:602	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	7	73	theme	injury	1548:1553	arg1	markers					1529:1535	morphological markers	1515:1535	morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD	1515:1590	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	0	74	theme	diverse	133:139	arg1	mice					161:164	genetically diverse Collaborative Cross mice	121:164	genetically diverse Collaborative Cross mice	121:164	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	2	75	from	factor	470:475	arg1	lipotoxicity					488:499	hepatic lipotoxicity	480:499	hepatic lipotoxicity	480:499	Recent studies have indicated that in addition to the total lipid quantities, changes in lipid composition are a determining factor in hepatic lipotoxicity.					
35858665	3	76	theme	fat/high	729:736	arg1	diet					754:757	a high fat/high sucrose (HF/HS) diet	722:757	a high fat/high sucrose (HF/HS) diet for 12 weeks	722:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	0	77	theme	Cross	155:159	arg1	mice					161:164	genetically diverse Collaborative Cross mice	121:164	genetically diverse Collaborative Cross mice	121:164	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	8	78	theme	NAFLD	1644:1648	arg1	models					1634:1639	different models	1624:1639	different models of NAFLD	1624:1648	These results demonstrate that different models of NAFLD are characterized by similar changes in the esterified fatty acid composition and that alterations in PUFA/MUFA ratios may serve as a diagnostic marker for NAFLD severity.					
35858665	7	79	theme	morphological	1515:1527	arg1	markers					1529:1535	morphological markers	1515:1535	morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD	1515:1590	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	6	80	theme	MUFA	1279:1282	arg1	enzyme					1300:1305	MUFA generating SCD1 enzyme	1279:1305	MUFA generating SCD1 enzyme	1279:1305	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	7	81	from	model	1577:1581	arg1	markers					1529:1535	morphological markers	1515:1535	morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD	1515:1590	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	3	82	theme	fatty	632:636	arg1	composition					643:653	the esterified fatty acid composition	617:653	the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks	617:770	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	8	83	theme	similar	1671:1677	arg1	changes					1679:1685	similar changes	1671:1685	similar changes in the esterified fatty acid composition	1671:1726	These results demonstrate that different models of NAFLD are characterized by similar changes in the esterified fatty acid composition and that alterations in PUFA/MUFA ratios may serve as a diagnostic marker for NAFLD severity.					
35858665	7	84	theme	HF/HS	1495:1499	arg1	diet					1501:1504	the HF/HS diet	1491:1504	the HF/HS diet	1491:1504	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	6	85	from	decrease	1324:1331	arg1	activity					1340:1347	the activity	1336:1347	the activity of PUFA generating FADS1 and FADS2 enzymes	1336:1390	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	6	85	from	decrease	1324:1331	arg1	activity					1267:1274	the estimated activity	1253:1274	the estimated activity of MUFA generating SCD1 enzyme	1253:1305	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	0	86	theme	nonalcoholic	85:96	arg1	disease					110:116	diet-induced nonalcoholic fatty liver disease	72:116	diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice	72:164	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	1	87	theme	species	321:327	arg1	accumulation					299:310	the excessive accumulation	285:310	the excessive accumulation of lipid species in hepatocytes	285:342	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	8	88	theme	esterified	1694:1703	arg1	composition					1716:1726	the esterified fatty acid composition	1690:1726	the esterified fatty acid composition	1690:1726	These results demonstrate that different models of NAFLD are characterized by similar changes in the esterified fatty acid composition and that alterations in PUFA/MUFA ratios may serve as a diagnostic marker for NAFLD severity.					
35858665	1	89	theme	Non-alcoholic	167:179	arg1	NAFLD					202:206	NAFLD	202:206	NAFLD	202:206	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	1	89	theme	Non-alcoholic	167:179	arg1	one					210:212	one	210:212	one	210:212	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	1	89	theme	Non-alcoholic	167:179	arg1	disease					193:199	Non-alcoholic fatty liver disease	167:199	Non-alcoholic fatty liver disease (NAFLD)	167:207	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	1	89	theme	Non-alcoholic	167:179	arg1	forms					233:237	the most common forms	217:237	the most common forms of chronic liver disease	217:262	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	7	90	theme	CC	1479:1480	arg1	mice					1482:1485	male and female CC mice	1463:1485	male and female CC mice fed the HF/HS diet	1463:1504	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	2	91	theme	determining	458:468	arg1	changes					423:429	changes	423:429	changes in lipid composition	423:450	Recent studies have indicated that in addition to the total lipid quantities, changes in lipid composition are a determining factor in hepatic lipotoxicity.					
35858665	2	91	theme	determining	458:468	arg1	factor					470:475	a determining factor	456:475	a determining factor in hepatic lipotoxicity	456:499	Recent studies have indicated that in addition to the total lipid quantities, changes in lipid composition are a determining factor in hepatic lipotoxicity.					
35858665	0	92	from	profiling	10:18	arg1	disease					110:116	diet-induced nonalcoholic fatty liver disease	72:116	diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice	72:164	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	8	93	from	alterations	1737:1747	arg1	ratios					1762:1767	PUFA/MUFA ratios	1752:1767	PUFA/MUFA ratios	1752:1767	These results demonstrate that different models of NAFLD are characterized by similar changes in the esterified fatty acid composition and that alterations in PUFA/MUFA ratios may serve as a diagnostic marker for NAFLD severity.					
35858665	0	94	theme	liver	104:108	arg1	disease					110:116	diet-induced nonalcoholic fatty liver disease	72:116	diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice	72:164	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	5	95	theme	mouse	1069:1073	arg1	model					1075:1079	a choline- and folate-deficient (CFD) mouse model	1031:1079	a choline- and folate-deficient (CFD) mouse model of NAFLD	1031:1088	Similar changes in MUFA and PUFA were observed in a choline- and folate-deficient (CFD) mouse model of NAFLD, as well as in hepatocytes treated in vitro with free fatty acids.					
35858665	8	96	theme	acid	1711:1714	arg1	composition					1716:1726	the esterified fatty acid composition	1690:1726	the esterified fatty acid composition	1690:1726	These results demonstrate that different models of NAFLD are characterized by similar changes in the esterified fatty acid composition and that alterations in PUFA/MUFA ratios may serve as a diagnostic marker for NAFLD severity.					
35858665	4	97	theme	fatty	909:913	arg1	MUFA					922:925	MUFA	922:925	MUFA	922:925	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	4	97	theme	fatty	909:913	arg1	acids					915:919	monounsaturated fatty acids	893:919	monounsaturated fatty acids (MUFA)	893:926	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	5	98	theme	choline-	1033:1040	arg1	model					1075:1079	a choline- and folate-deficient (CFD) mouse model	1031:1079	a choline- and folate-deficient (CFD) mouse model of NAFLD	1031:1088	Similar changes in MUFA and PUFA were observed in a choline- and folate-deficient (CFD) mouse model of NAFLD, as well as in hepatocytes treated in vitro with free fatty acids.					
35858665	4	99	from	decreases	932:940	arg1	acids					967:971	polyunsaturated fatty acids	945:971	polyunsaturated fatty acids (PUFA)	945:978	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	4	99	from	decreases	932:940	arg1	MUFA					922:925	MUFA	922:925	MUFA	922:925	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	4	99	from	decreases	932:940	arg1	acids					915:919	monounsaturated fatty acids	893:919	monounsaturated fatty acids (MUFA)	893:926	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	4	99	from	decreases	932:940	arg1	PUFA					974:977	PUFA	974:977	PUFA	974:977	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	6	100	theme	PUFA	1352:1355	arg1	activity					1340:1347	the activity	1336:1347	the activity of PUFA generating FADS1 and FADS2 enzymes	1336:1390	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	5	101	theme	folate-deficient	1046:1061	arg1	model					1075:1079	a choline- and folate-deficient (CFD) mouse model	1031:1079	a choline- and folate-deficient (CFD) mouse model of NAFLD	1031:1088	Similar changes in MUFA and PUFA were observed in a choline- and folate-deficient (CFD) mouse model of NAFLD, as well as in hepatocytes treated in vitro with free fatty acids.					
35858665	7	102	from	injury	1548:1553	arg1	model					1577:1581	CFD diet-fed mouse model	1558:1581	CFD diet-fed mouse model of NAFLD	1558:1590	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	7	103	theme	NAFLD	1586:1590	arg1	model					1577:1581	CFD diet-fed mouse model	1558:1581	CFD diet-fed mouse model of NAFLD	1558:1590	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	8	104	theme	PUFA/MUFA	1752:1760	arg1	ratios					1762:1767	PUFA/MUFA ratios	1752:1767	PUFA/MUFA ratios	1752:1767	These results demonstrate that different models of NAFLD are characterized by similar changes in the esterified fatty acid composition and that alterations in PUFA/MUFA ratios may serve as a diagnostic marker for NAFLD severity.					
35858665	2	105	theme	total	399:403	arg1	quantities					411:420	the total lipid quantities	395:420	the total lipid quantities	395:420	Recent studies have indicated that in addition to the total lipid quantities, changes in lipid composition are a determining factor in hepatic lipotoxicity.					
35858665	0	106	theme	esterified	35:44	arg1	composition					57:67	the hepatic esterified fatty acid composition	23:67	the hepatic esterified fatty acid composition	23:67	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	7	107	theme	male	1463:1466	arg1	mice					1482:1485	male and female CC mice	1463:1485	male and female CC mice fed the HF/HS diet	1463:1504	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	7	108	theme	diet-fed	1562:1569	arg1	model					1577:1581	CFD diet-fed mouse model	1558:1581	CFD diet-fed mouse model of NAFLD	1558:1590	PUFA/MUFA ratios were inversely correlated with lipid accumulation in male and female CC mice fed the HF/HS diet and with morphological markers of hepatic injury in CFD diet-fed mouse model of NAFLD.					
35858665	4	109	theme	polyunsaturated	945:959	arg1	acids					967:971	polyunsaturated fatty acids	945:971	polyunsaturated fatty acids (PUFA)	945:978	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	4	109	theme	polyunsaturated	945:959	arg1	PUFA					974:977	PUFA	974:977	PUFA	974:977	Changes in lipid composition were found in all strains after the HF/HS diet, most notably characterized by increases in monounsaturated fatty acids (MUFA) and decreases in polyunsaturated fatty acids (PUFA).					
35858665	1	110	theme	common	226:231	arg1	forms					233:237	the most common forms	217:237	the most common forms of chronic liver disease	217:262	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	0	111	theme	acid	52:55	arg1	composition					57:67	the hepatic esterified fatty acid composition	23:67	the hepatic esterified fatty acid composition	23:67	Lipidomic profiling of the hepatic esterified fatty acid composition in diet-induced nonalcoholic fatty liver disease in genetically diverse Collaborative Cross mice.					
35858665	1	112	theme	excessive	289:297	arg1	accumulation					299:310	the excessive accumulation	285:310	the excessive accumulation of lipid species in hepatocytes	285:342	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	2	113	theme	lipid	434:438	arg1	composition					440:450	lipid composition	434:450	lipid composition	434:450	Recent studies have indicated that in addition to the total lipid quantities, changes in lipid composition are a determining factor in hepatic lipotoxicity.					
35858665	5	114	theme	free	1139:1142	arg1	acids					1150:1154	free fatty acids	1139:1154	free fatty acids	1139:1154	Similar changes in MUFA and PUFA were observed in a choline- and folate-deficient (CFD) mouse model of NAFLD, as well as in hepatocytes treated in vitro with free fatty acids.					
35858665	3	115	theme	ultra-high	508:517	arg1	chromatography					538:551	ultra-high performance liquid chromatography	508:551	ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry	508:602	Using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry, we analyzed the esterified fatty acid composition in 24 strains of male and female Collaborative Cross (CC) mice fed a high fat/high sucrose (HF/HS) diet for 12 weeks.					
35858665	6	116	theme	FADS2	1378:1382	arg1	enzymes					1384:1390	FADS1 and FADS2 enzymes	1368:1390	FADS1 and FADS2 enzymes	1368:1390	Analysis of fatty acid composition revealed that alterations were accompanied by an increase in the estimated activity of MUFA generating SCD1 enzyme and an estimated decrease in the activity of PUFA generating FADS1 and FADS2 enzymes.					
35858665	1	117	theme	forms	233:237	arg1	forms					233:237	the most common forms	217:237	the most common forms of chronic liver disease	217:262	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	1	117	theme	forms	233:237	arg1	one					210:212	one	210:212	one	210:212	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
35858665	1	117	theme	forms	233:237	arg1	disease					193:199	Non-alcoholic fatty liver disease	167:199	Non-alcoholic fatty liver disease (NAFLD)	167:207	Non-alcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is characterized by the excessive accumulation of lipid species in hepatocytes.					
36423755	5	0	theme	swelled	833:839	arg1	4.8-5.0 nm					860:869	4.8-5.0 nm	860:869	4.8-5.0 nm	860:869	Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation favored the enhanced hydrolysis, rather than the crystalline cellulose II.					
36423755	5	0	theme	swelled	833:839	arg1	sizes					853:857	swelled crystallite sizes	833:857	Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation	790:904	Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation favored the enhanced hydrolysis, rather than the crystalline cellulose II.					
36423755	4	1	theme	matrix	782:787	arg1	removal					745:751	the removal	741:751	the removal of lignin and hemicelluloses matrix	741:787	Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix.					
36423755	5	2	theme	crystallite	841:851	arg1	4.8-5.0 nm					860:869	4.8-5.0 nm	860:869	4.8-5.0 nm	860:869	Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation favored the enhanced hydrolysis, rather than the crystalline cellulose II.					
36423755	5	2	theme	crystallite	841:851	arg1	sizes					853:857	swelled crystallite sizes	833:857	Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation	790:904	Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation favored the enhanced hydrolysis, rather than the crystalline cellulose II.					
36423755	6	3	theme	well-distributed	991:1006	arg1	separation					1008:1017	well-distributed separation	991:1017	well-distributed separation	991:1017	Moreover, well-distributed separation and fibrillation of cellulose elementary fibrils also contributed to glucose yield promotion.					
36423755	3	4	theme	alkaline	480:487	arg1	treatments					489:498	continuous mild alkaline treatments	464:498	continuous mild alkaline treatments	464:498	Delignification and continuous mild alkaline treatments were applied for deconstruction of rattan cane (Calamus simplicifolius) followed by cellulase enzymatic hydrolysis.					
36423755	0	5	theme	structural	99:108	arg1	variations					110:119	The supramolecular structural variations	80:119	Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.	0:133	Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.					
36423755	2	6	theme	hardwood-like	306:318	arg1	composition					329:339	hardwood-like chemical composition	306:339	hardwood-like chemical composition	306:339	Herein, rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition was used as model of lignocellulose to determine its recalcitrance inhibiting efficient bioconversion.					
36423755	6	7	theme	yield	1096:1100	arg1	promotion					1102:1110	glucose yield promotion	1088:1110	glucose yield promotion	1088:1110	Moreover, well-distributed separation and fibrillation of cellulose elementary fibrils also contributed to glucose yield promotion.					
36423755	0	8	theme	supramolecular	84:97	arg1	variations					110:119	The supramolecular structural variations	80:119	Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.	0:133	Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.					
36423755	4	9	theme	enhanced	706:713	arg1	hydrolysis					715:724	the enhanced hydrolysis	702:724	the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix	702:787	Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix.					
36423755	6	10	theme	glucose	1088:1094	arg1	promotion					1102:1110	glucose yield promotion	1088:1110	glucose yield promotion	1088:1110	Moreover, well-distributed separation and fibrillation of cellulose elementary fibrils also contributed to glucose yield promotion.					
36423755	4	11	theme	hemicelluloses	767:780	arg1	matrix					782:787	hemicelluloses matrix	767:787	hemicelluloses matrix	767:787	Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix.					
36423755	5	12	theme	enhanced	918:925	arg1	hydrolysis					927:936	the enhanced hydrolysis	914:936	the enhanced hydrolysis	914:936	Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation favored the enhanced hydrolysis, rather than the crystalline cellulose II.					
36423755	3	13	theme	rattan	535:540	arg1	cane					542:545	rattan cane	535:545	rattan cane (Calamus simplicifolius)	535:570	Delignification and continuous mild alkaline treatments were applied for deconstruction of rattan cane (Calamus simplicifolius) followed by cellulase enzymatic hydrolysis.					
36423755	3	14	theme	enzymatic	594:602	arg1	hydrolysis					604:613	cellulase enzymatic hydrolysis	584:613	cellulase enzymatic hydrolysis	584:613	Delignification and continuous mild alkaline treatments were applied for deconstruction of rattan cane (Calamus simplicifolius) followed by cellulase enzymatic hydrolysis.					
36423755	1	15	theme	sufficient	181:190	arg1	utilization					192:202	sufficient utilization	181:202	sufficient utilization of biomass to renewable energy	181:233	Global energy concerns urged us to search for sufficient utilization of biomass to renewable energy.					
36423755	5	16	theme	Lowered	790:796	arg1	crystallinity					798:810	Lowered crystallinity	790:810	Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation	790:904	Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation favored the enhanced hydrolysis, rather than the crystalline cellulose II.					
36423755	5	16	theme	Lowered	790:796	arg1	%					819:819	50-65 %	813:819	50-65 %	813:819	Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation favored the enhanced hydrolysis, rather than the crystalline cellulose II.					
36423755	3	17	theme	continuous	464:473	arg1	treatments					489:498	continuous mild alkaline treatments	464:498	continuous mild alkaline treatments	464:498	Delignification and continuous mild alkaline treatments were applied for deconstruction of rattan cane (Calamus simplicifolius) followed by cellulase enzymatic hydrolysis.					
36423755	2	18	theme	species-like	281:292	arg1	anatomy					294:300	herbaceous species-like anatomy	270:300	herbaceous species-like anatomy	270:300	Herein, rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition was used as model of lignocellulose to determine its recalcitrance inhibiting efficient bioconversion.					
36423755	2	19	theme	efficient	419:427	arg1	bioconversion					429:441	efficient bioconversion	419:441	efficient bioconversion	419:441	Herein, rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition was used as model of lignocellulose to determine its recalcitrance inhibiting efficient bioconversion.					
36423755	5	20	theme	allomorphic	879:889	arg1	transformation					891:904	allomorphic transformation	879:904	allomorphic transformation	879:904	Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation favored the enhanced hydrolysis, rather than the crystalline cellulose II.					
36423755	4	21	theme	structural	641:650	arg1	reason					691:696	the major reason	681:696	the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix	681:787	Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix.					
36423755	4	21	theme	structural	641:650	arg1	variations					652:661	Cellulose supramolecular structural variations	616:661	Cellulose supramolecular structural variations	616:661	Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix.					
36423755	7	22	theme	lignocellulosic	1195:1209	arg1	biomass					1211:1217	lignocellulosic biomass	1195:1217	lignocellulosic biomass	1195:1217	The study will provide new insights to the strategy to efficient bioconversion of lignocellulosic biomass.					
36423755	7	23	theme	biomass	1211:1217	arg1	bioconversion					1178:1190	efficient bioconversion	1168:1190	efficient bioconversion of lignocellulosic biomass	1168:1217	The study will provide new insights to the strategy to efficient bioconversion of lignocellulosic biomass.					
36423755	5	24	theme	crystalline	955:965	arg1	cellulose					967:975	the crystalline cellulose II	951:978	the crystalline cellulose II	951:978	Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation favored the enhanced hydrolysis, rather than the crystalline cellulose II.					
36423755	4	25	theme	supramolecular	626:639	arg1	reason					691:696	the major reason	681:696	the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix	681:787	Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix.					
36423755	4	25	theme	supramolecular	626:639	arg1	variations					652:661	Cellulose supramolecular structural variations	616:661	Cellulose supramolecular structural variations	616:661	Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix.					
36423755	7	26	theme	efficient	1168:1176	arg1	bioconversion					1178:1190	efficient bioconversion	1168:1190	efficient bioconversion of lignocellulosic biomass	1168:1217	The study will provide new insights to the strategy to efficient bioconversion of lignocellulosic biomass.					
36423755	1	27	dep	concerns	149:156	arg1	urged					158:162	urged	158:162	concerns urged us to search for sufficient utilization of biomass to renewable energy	149:233	Global energy concerns urged us to search for sufficient utilization of biomass to renewable energy.					
36423755	0	28	theme	key	14:16	arg1	factors					18:24	key factors	14:24	key factors affecting bioconversion efficiency of rattan biomass	14:77	Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.					
36423755	0	29	theme	cellulose	124:132	arg1	variations					110:119	The supramolecular structural variations	80:119	Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.	0:133	Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.					
36423755	2	30	theme	chemical	320:327	arg1	composition					329:339	hardwood-like chemical composition	306:339	hardwood-like chemical composition	306:339	Herein, rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition was used as model of lignocellulose to determine its recalcitrance inhibiting efficient bioconversion.					
36423755	1	31	theme	biomass	207:213	arg1	utilization					192:202	sufficient utilization	181:202	sufficient utilization of biomass to renewable energy	181:233	Global energy concerns urged us to search for sufficient utilization of biomass to renewable energy.					
36423755	0	32	theme	bioconversion	36:48	arg1	efficiency					50:59	bioconversion efficiency	36:59	bioconversion efficiency of rattan biomass	36:77	Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.					
36423755	6	33	theme	fibrils	1060:1066	arg1	fibrillation					1023:1034	fibrillation	1023:1034	fibrillation	1023:1034	Moreover, well-distributed separation and fibrillation of cellulose elementary fibrils also contributed to glucose yield promotion.					
36423755	6	33	theme	fibrils	1060:1066	arg1	separation					1008:1017	well-distributed separation	991:1017	well-distributed separation	991:1017	Moreover, well-distributed separation and fibrillation of cellulose elementary fibrils also contributed to glucose yield promotion.					
36423755	3	34	theme	mild	475:478	arg1	treatments					489:498	continuous mild alkaline treatments	464:498	continuous mild alkaline treatments	464:498	Delignification and continuous mild alkaline treatments were applied for deconstruction of rattan cane (Calamus simplicifolius) followed by cellulase enzymatic hydrolysis.					
36423755	6	35	theme	elementary	1049:1058	arg1	fibrils					1060:1066	cellulose elementary fibrils	1039:1066	cellulose elementary fibrils	1039:1066	Moreover, well-distributed separation and fibrillation of cellulose elementary fibrils also contributed to glucose yield promotion.					
36423755	1	36	theme	renewable	218:226	arg1	energy					228:233	renewable energy	218:233	renewable energy	218:233	Global energy concerns urged us to search for sufficient utilization of biomass to renewable energy.					
36423755	6	37	theme	cellulose	1039:1047	arg1	fibrils					1060:1066	cellulose elementary fibrils	1039:1066	cellulose elementary fibrils	1039:1066	Moreover, well-distributed separation and fibrillation of cellulose elementary fibrils also contributed to glucose yield promotion.					
36423755	2	38	theme	lignocellulose	362:375	arg1	biomass					251:257	rattan biomass	244:257	rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition	244:339	Herein, rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition was used as model of lignocellulose to determine its recalcitrance inhibiting efficient bioconversion.					
36423755	2	38	theme	lignocellulose	362:375	arg1	model					353:357	model	353:357	model of lignocellulose	353:375	Herein, rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition was used as model of lignocellulose to determine its recalcitrance inhibiting efficient bioconversion.					
36423755	4	39	theme	major	685:689	arg1	reason					691:696	the major reason	681:696	the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix	681:787	Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix.					
36423755	4	39	theme	major	685:689	arg1	variations					652:661	Cellulose supramolecular structural variations	616:661	Cellulose supramolecular structural variations	616:661	Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix.					
36423755	4	40	theme	Cellulose	616:624	arg1	reason					691:696	the major reason	681:696	the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix	681:787	Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix.					
36423755	4	40	theme	Cellulose	616:624	arg1	variations					652:661	Cellulose supramolecular structural variations	616:661	Cellulose supramolecular structural variations	616:661	Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix.					
36423755	3	41	dep	applied	505:511	arg1	followed					572:579	followed	572:579	followed by cellulase enzymatic hydrolysis	572:613	Delignification and continuous mild alkaline treatments were applied for deconstruction of rattan cane (Calamus simplicifolius) followed by cellulase enzymatic hydrolysis.					
36423755	0	42	dep	Insights	0:7	arg1	variations					110:119	The supramolecular structural variations	80:119	Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.	0:133	Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.					
36423755	7	43	theme	new	1136:1138	arg1	insights					1140:1147	new insights	1136:1147	new insights	1136:1147	The study will provide new insights to the strategy to efficient bioconversion of lignocellulosic biomass.					
36423755	2	44	theme	herbaceous	270:279	arg1	anatomy					294:300	herbaceous species-like anatomy	270:300	herbaceous species-like anatomy	270:300	Herein, rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition was used as model of lignocellulose to determine its recalcitrance inhibiting efficient bioconversion.					
36423755	1	45	theme	Global	135:140	arg1	energy					142:147	Global energy	135:147	Global energy	135:147	Global energy concerns urged us to search for sufficient utilization of biomass to renewable energy.					
36423755	3	46	dep	cane	542:545	arg1	simplicifolius					556:569	Calamus simplicifolius	548:569	Calamus simplicifolius	548:569	Delignification and continuous mild alkaline treatments were applied for deconstruction of rattan cane (Calamus simplicifolius) followed by cellulase enzymatic hydrolysis.					
36423755	4	47	theme	lignin	756:761	arg1	removal					745:751	the removal	741:751	the removal of lignin and hemicelluloses matrix	741:787	Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix.					
36423755	3	48	theme	cane	542:545	arg1	deconstruction					517:530	deconstruction	517:530	deconstruction of rattan cane (Calamus simplicifolius)	517:570	Delignification and continuous mild alkaline treatments were applied for deconstruction of rattan cane (Calamus simplicifolius) followed by cellulase enzymatic hydrolysis.					
36423755	0	49	theme	biomass	71:77	arg1	efficiency					50:59	bioconversion efficiency	36:59	bioconversion efficiency of rattan biomass	36:77	Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.					
36423755	2	50	used	used	345:348	arg2	biomass					251:257	rattan biomass	244:257	rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition	244:339	Herein, rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition was used as model of lignocellulose to determine its recalcitrance inhibiting efficient bioconversion.					
36423755	2	50	used	used	345:348	arg2	model					353:357	model	353:357	model of lignocellulose	353:375	Herein, rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition was used as model of lignocellulose to determine its recalcitrance inhibiting efficient bioconversion.					
36423755	0	51	theme	rattan	64:69	arg1	biomass					71:77	rattan biomass	64:77	rattan biomass	64:77	Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.					
36423755	2	52	theme	rattan	244:249	arg1	biomass					251:257	rattan biomass	244:257	rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition	244:339	Herein, rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition was used as model of lignocellulose to determine its recalcitrance inhibiting efficient bioconversion.					
36423755	2	52	theme	rattan	244:249	arg1	model					353:357	model	353:357	model of lignocellulose	353:375	Herein, rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition was used as model of lignocellulose to determine its recalcitrance inhibiting efficient bioconversion.					
36423755	3	53	theme	cellulase	584:592	arg1	hydrolysis					604:613	cellulase enzymatic hydrolysis	584:613	cellulase enzymatic hydrolysis	584:613	Delignification and continuous mild alkaline treatments were applied for deconstruction of rattan cane (Calamus simplicifolius) followed by cellulase enzymatic hydrolysis.					
36113451	12	0	theme	proper	1977:1982	arg1	interaction					1984:1994	the proper interaction	1973:1994	the proper interaction between GO/NBG with chitosan-gelatin blend	1973:2037	DSC and TGA data revealed the thermal stability of GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend.					
36113451	6	1	theme	XRD	1159:1161	arg1	data					1163:1166	XRD data	1159:1166	XRD data	1159:1166	XRD data revealed the successful incorporation of NBG and GO and distribution across the scaffolds.					
36113451	17	2	theme	phosphatase	2481:2491	arg1	activity					2493:2500	Alkaline phosphatase activity	2472:2500	Alkaline phosphatase activity	2472:2500	Alkaline phosphatase activity suggested improved osteogenic differentiation of MG-63 cells over GO based scaffolds and this was due to the osteogenic potential of NBG and GO present in the scaffolds.					
36113451	14	3	theme	suitable	2173:2180	arg1	attachment					2187:2196	suitable cell attachment	2173:2196	suitable cell attachment	2173:2196	It revealed suitable cell attachment and proliferation of cells compared to the Ch-G-NBG scaffold.					
36113451	3	4	theme	resolution	733:742	arg1	diffraction					709:719	x-ray diffraction	703:719	x-ray diffraction (XRD)	703:725	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	4	theme	resolution	733:742	arg1	microscope					762:771	high resolution scanning electron microscope	728:771	high resolution scanning electron microscope	728:771	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	2	5	theme	nanocomposite	345:357	arg1	chitosan/gelatin/nanobioglass/GO					393:424	chitosan/gelatin/nanobioglass/GO	393:424	chitosan/gelatin/nanobioglass/GO	393:424	In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties.					
36113451	2	5	theme	nanocomposite	345:357	arg1	scaffolds					382:390	nanocomposite biocompatible GO-based scaffolds	345:390	nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO	345:437	In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties.					
36113451	1	6	theme	electrical	244:253	arg1	conductivity					255:266	excellent electrical conductivity	234:266	excellent electrical conductivity	234:266	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	16	7	theme	cell	2405:2408	arg1	proliferation					2410:2422	the cell proliferation	2401:2422	the cell proliferation	2401:2422	But in case of Ch-G-NBG-90%GO scaffold, the cell proliferation was reduced when compared to control scaffolds.					
36113451	8	8	from	temperature	1544:1554	arg1	stable					1529:1534	stable	1529:1534	stable	1529:1534	The studies also reveal that after GO incorporation, the weight loss reduced (0.11 ± 0.02-0.095 ± 0.03), scaffolds were firmly stable at room temperature even after a long duration of 28 d.					
36113451	18	9	theme	nano-biocomposite	2700:2716	arg1	scaffold					2718:2725	the nano-biocomposite scaffold	2696:2725	the nano-biocomposite scaffold	2696:2725	Based on these results, the nano-biocomposite scaffold appears to have the potential for utilization in bone tissue restoration, replacement and regeneration.					
36113451	15	10	theme	MTT	2260:2262	arg1	assay					2264:2268	MTT assay	2260:2268	MTT assay	2260:2268	MTT assay showed that Ch-G-NBG-GO scaffold below 90% GO concentration possess best biocompatibility.					
36113451	12	11	theme	scaffolds	1956:1964	arg1	stability					1927:1935	the thermal stability	1915:1935	the thermal stability of GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend	1915:2037	DSC and TGA data revealed the thermal stability of GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend.					
36113451	3	12	theme	prepared	583:590	arg1	composites					604:613	The prepared Ch-G-NBG-GO composites	579:613	The prepared Ch-G-NBG-GO composites	579:613	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	13	theme	thermogravimetric	815:831	arg1	TGA					843:845	TGA	843:845	TGA	843:845	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	13	theme	thermogravimetric	815:831	arg1	analysis					833:840	thermogravimetric analysis	815:840	thermogravimetric analysis (TGA)	815:846	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	1	14	theme	engineering	169:179	arg1	field					149:153	the field	145:153	the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility	145:325	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	9	15	theme	mechanical	1682:1691	arg1	strength					1693:1700	the mechanical strength	1678:1700	the mechanical strength of these scaffolds	1678:1719	The crystallinity added to the scaffolds due to addition of GO nanoparticles improved the mechanical strength of these scaffolds.					
36113451	1	16	theme	Graphene	92:99	arg1	oxide					101:105	Graphene oxide	92:105	Graphene oxide (GO)	92:110	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	13	17	theme	bone	2069:2072	arg1	engineering					2081:2091	bone tissue engineering	2069:2091	bone tissue engineering	2069:2091	The scaffold's potential for bone tissue engineering was evaluated by testing its cytocompatibility for MG-63 cell line.					
36113451	10	18	dep	addition	1786:1793	arg1	GO					1783:1784	GO	1783:1784	GO	1783:1784	The compressive modulus changed from (5.7 to 8.51) MPa after GO addition.					
36113451	18	19	contain	have	2738:2741	arg1	scaffold					2718:2725	the nano-biocomposite scaffold	2696:2725	the nano-biocomposite scaffold	2696:2725	Based on these results, the nano-biocomposite scaffold appears to have the potential for utilization in bone tissue restoration, replacement and regeneration.					
36113451	18	19	contain	have	2738:2741	arg2	potential					2747:2755	the potential	2743:2755	the potential for utilization	2743:2771	Based on these results, the nano-biocomposite scaffold appears to have the potential for utilization in bone tissue restoration, replacement and regeneration.					
36113451	5	20	theme	control	1140:1146	arg1	μm					1108:1109	between 90 and 120 μm	1089:1109	between 90 and 120 μm	1089:1109	Average pore size for the Ch-G-NBG-GO scaffolds were in between 90 and 120 μm, which was very close to the control scaffolds.					
36113451	5	20	theme	control	1140:1146	arg1	scaffolds					1148:1156	the control scaffolds	1136:1156	the control scaffolds	1136:1156	Average pore size for the Ch-G-NBG-GO scaffolds were in between 90 and 120 μm, which was very close to the control scaffolds.					
36113451	16	21	dep	scaffold	2391:2398	arg1	GO					2388:2389	GO	2388:2389	GO	2388:2389	But in case of Ch-G-NBG-90%GO scaffold, the cell proliferation was reduced when compared to control scaffolds.					
36113451	9	22	theme	scaffolds	1711:1719	arg1	strength					1693:1700	the mechanical strength	1678:1700	the mechanical strength of these scaffolds	1678:1719	The crystallinity added to the scaffolds due to addition of GO nanoparticles improved the mechanical strength of these scaffolds.					
36113451	17	23	from	scaffolds	2661:2669	arg1	present					2646:2652	present	2646:2652	present	2646:2652	Alkaline phosphatase activity suggested improved osteogenic differentiation of MG-63 cells over GO based scaffolds and this was due to the osteogenic potential of NBG and GO present in the scaffolds.					
36113451	4	24	theme	interconnected	990:1003	arg1	network					1005:1011	the porous interconnected network	979:1011	the porous interconnected network of scaffold formed	979:1030	The morphological analysis showed the porous interconnected network of scaffold formed.					
36113451	7	25	theme	%	1390:1390	arg1	porosity					1392:1399	79% porosity	1388:1399	79% porosity	1388:1399	Porosity of the fabricated scaffolds were in the range between 75.3% and 77.3% which was very close to the control scaffold with 79% porosity.					
36113451	11	26	dep	%	1862:1862	arg1	GO					1863:1864	GO	1863:1864	GO	1863:1864	Swelling ratio changed drastically especially in case of Ch-NBG-90%GO (4.9 ± 0.04-4 ± 0.01).					
36113451	7	27	with	scaffold	1374:1381	arg1	porosity					1392:1399	79% porosity	1388:1399	79% porosity	1388:1399	Porosity of the fabricated scaffolds were in the range between 75.3% and 77.3% which was very close to the control scaffold with 79% porosity.					
36113451	8	28	dep	incorporation	1440:1452	arg1	GO					1437:1438	GO	1437:1438	GO	1437:1438	The studies also reveal that after GO incorporation, the weight loss reduced (0.11 ± 0.02-0.095 ± 0.03), scaffolds were firmly stable at room temperature even after a long duration of 28 d.					
36113451	1	29	dep	oxide	101:105	arg1	GO					108:109	GO	108:109	GO	108:109	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	3	30	theme	energy-dispersive	849:865	arg1	Spectroscopy					873:884	energy-dispersive x-ray Spectroscopy	849:884	energy-dispersive x-ray Spectroscopy	849:884	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	31	theme	scanning	904:911	arg1	DSC					926:928	DSC	926:928	DSC	926:928	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	31	theme	scanning	904:911	arg1	colorimetry					913:923	differential scanning colorimetry	891:923	differential scanning colorimetry (DSC)	891:929	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	0	32	theme	tissue	71:76	arg1	regeneration					78:89	bone tissue regeneration	66:89	bone tissue regeneration	66:89	Generation of graphene oxide and nano-bioglass based scaffold for bone tissue regeneration.					
36113451	15	33	dep	%	2311:2311	arg1	GO					2313:2314	GO	2313:2314	GO concentration	2313:2328	MTT assay showed that Ch-G-NBG-GO scaffold below 90% GO concentration possess best biocompatibility.					
36113451	17	34	theme	osteogenic	2521:2530	arg1	differentiation					2532:2546	improved osteogenic differentiation	2512:2546	improved osteogenic differentiation of MG-63 cells	2512:2561	Alkaline phosphatase activity suggested improved osteogenic differentiation of MG-63 cells over GO based scaffolds and this was due to the osteogenic potential of NBG and GO present in the scaffolds.					
36113451	2	35	theme	biological	556:565	arg1	properties					567:576	various physico-chemical and biological properties	527:576	properties	567:576	In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties.					
36113451	14	36	theme	Ch-G-NBG	2241:2248	arg1	scaffold					2250:2257	the Ch-G-NBG scaffold	2237:2257	the Ch-G-NBG scaffold	2237:2257	It revealed suitable cell attachment and proliferation of cells compared to the Ch-G-NBG scaffold.					
36113451	11	37	theme	±	1871:1871	arg1	%					1862:1862	Ch-NBG-90%GO	1853:1864	Ch-NBG-90%GO (4.9 ± 0.04-4 ± 0.01)	1853:1886	Swelling ratio changed drastically especially in case of Ch-NBG-90%GO (4.9 ± 0.04-4 ± 0.01).					
36113451	11	37	theme	±	1871:1871	arg1	±					1880:1880	4.9 ± 0.04-4 ± 0.01	1867:1885	4.9 ± 0.04-4 ± 0.01	1867:1885	Swelling ratio changed drastically especially in case of Ch-NBG-90%GO (4.9 ± 0.04-4 ± 0.01).					
36113451	17	38	theme	cells	2557:2561	arg1	differentiation					2532:2546	improved osteogenic differentiation	2512:2546	improved osteogenic differentiation of MG-63 cells	2512:2561	Alkaline phosphatase activity suggested improved osteogenic differentiation of MG-63 cells over GO based scaffolds and this was due to the osteogenic potential of NBG and GO present in the scaffolds.					
36113451	1	39	theme	high	269:272	arg1	transparency					282:293	high optical transparency	269:293	high optical transparency	269:293	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	5	40	theme	Average	1033:1039	arg1	size					1046:1049	Average pore size	1033:1049	Average pore size for the Ch-G-NBG-GO scaffolds	1033:1079	Average pore size for the Ch-G-NBG-GO scaffolds were in between 90 and 120 μm, which was very close to the control scaffolds.					
36113451	2	41	theme	drying	483:488	arg1	technique					490:498	freeze drying technique	476:498	freeze drying technique (-40 °C)	476:507	In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties.					
36113451	2	41	theme	drying	483:488	arg1	°C					505:506	-40 °C	501:506	-40 °C	501:506	In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties.					
36113451	12	42	dep	scaffolds	1956:1964	arg1	GO					1940:1941	GO	1940:1941	GO	1940:1941	DSC and TGA data revealed the thermal stability of GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend.					
36113451	14	43	theme	cells	2219:2223	arg1	proliferation					2202:2214	proliferation	2202:2214	proliferation	2202:2214	It revealed suitable cell attachment and proliferation of cells compared to the Ch-G-NBG scaffold.					
36113451	14	43	theme	cells	2219:2223	arg1	attachment					2187:2196	suitable cell attachment	2173:2196	suitable cell attachment	2173:2196	It revealed suitable cell attachment and proliferation of cells compared to the Ch-G-NBG scaffold.					
36113451	0	44	theme	graphene	14:21	arg1	oxide					23:27	graphene oxide	14:27	graphene oxide	14:27	Generation of graphene oxide and nano-bioglass based scaffold for bone tissue regeneration.					
36113451	16	45	theme	control	2453:2459	arg1	scaffolds					2461:2469	control scaffolds	2453:2469	control scaffolds	2453:2469	But in case of Ch-G-NBG-90%GO scaffold, the cell proliferation was reduced when compared to control scaffolds.					
36113451	15	46	contain	possess	2330:2336	arg2	biocompatibility					2343:2358	biocompatibility	2343:2358	biocompatibility	2343:2358	MTT assay showed that Ch-G-NBG-GO scaffold below 90% GO concentration possess best biocompatibility.					
36113451	15	46	contain	possess	2330:2336	arg1	scaffold					2294:2301	Ch-G-NBG-GO scaffold	2282:2301	Ch-G-NBG-GO scaffold below 90% GO concentration	2282:2328	MTT assay showed that Ch-G-NBG-GO scaffold below 90% GO concentration possess best biocompatibility.					
36113451	8	47	theme	long	1569:1572	arg1	duration					1574:1581	a long duration	1567:1581	a long duration of 28 d	1567:1589	The studies also reveal that after GO incorporation, the weight loss reduced (0.11 ± 0.02-0.095 ± 0.03), scaffolds were firmly stable at room temperature even after a long duration of 28 d.					
36113451	3	48	theme	x-ray	703:707	arg1	diffraction					709:719	x-ray diffraction	703:719	x-ray diffraction (XRD)	703:725	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	48	theme	x-ray	703:707	arg1	Fourier					774:780	Fourier	774:780	Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively	774:942	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	48	theme	x-ray	703:707	arg1	microscope					762:771	high resolution scanning electron microscope	728:771	high resolution scanning electron microscope	728:771	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	48	theme	x-ray	703:707	arg1	XRD					722:724	XRD	722:724	XRD	722:724	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	9	49	dep	nanoparticles	1655:1667	arg1	GO					1652:1653	GO	1652:1653	GO	1652:1653	The crystallinity added to the scaffolds due to addition of GO nanoparticles improved the mechanical strength of these scaffolds.					
36113451	8	50	dep	reduced	1471:1477	arg1	0.03					1500:1503	0.03	1500:1503	0.03	1500:1503	The studies also reveal that after GO incorporation, the weight loss reduced (0.11 ± 0.02-0.095 ± 0.03), scaffolds were firmly stable at room temperature even after a long duration of 28 d.					
36113451	3	51	theme	high	728:731	arg1	diffraction					709:719	x-ray diffraction	703:719	x-ray diffraction (XRD)	703:725	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	51	theme	high	728:731	arg1	microscope					762:771	high resolution scanning electron microscope	728:771	high resolution scanning electron microscope	728:771	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	1	52	theme	high	208:211	arg1	strength					224:231	its exceptionally high mechanical strength	190:231	its exceptionally high mechanical strength	190:231	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	8	53	theme	d	1589:1589	arg1	duration					1574:1581	a long duration	1567:1581	a long duration of 28 d	1567:1589	The studies also reveal that after GO incorporation, the weight loss reduced (0.11 ± 0.02-0.095 ± 0.03), scaffolds were firmly stable at room temperature even after a long duration of 28 d.					
36113451	8	54	theme	weight	1459:1464	arg1	loss					1466:1469	the weight loss	1455:1469	the weight loss	1455:1469	The studies also reveal that after GO incorporation, the weight loss reduced (0.11 ± 0.02-0.095 ± 0.03), scaffolds were firmly stable at room temperature even after a long duration of 28 d.					
36113451	16	55	theme	Ch-G-NBG-90	2376:2386	arg1	scaffold					2391:2398	Ch-G-NBG-90%GO scaffold	2376:2398	Ch-G-NBG-90%GO scaffold	2376:2398	But in case of Ch-G-NBG-90%GO scaffold, the cell proliferation was reduced when compared to control scaffolds.					
36113451	2	56	theme	scaffolds	382:390	arg1	Ch-G-NBG-GO					427:437	nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO	345:437	nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO	345:437	In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties.					
36113451	13	57	theme	MG-63	2144:2148	arg1	line					2155:2158	MG-63 cell line	2144:2158	MG-63 cell line	2144:2158	The scaffold's potential for bone tissue engineering was evaluated by testing its cytocompatibility for MG-63 cell line.					
36113451	12	58	theme	due	1966:1968	arg1	scaffolds					1956:1964	incorporated scaffolds	1943:1964	GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend	1940:2037	DSC and TGA data revealed the thermal stability of GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend.					
36113451	17	59	theme	Alkaline	2472:2479	arg1	phosphatase					2481:2491	Alkaline phosphatase	2472:2491	Alkaline phosphatase activity	2472:2500	Alkaline phosphatase activity suggested improved osteogenic differentiation of MG-63 cells over GO based scaffolds and this was due to the osteogenic potential of NBG and GO present in the scaffolds.					
36113451	1	60	theme	favorable	300:308	arg1	biocompatibility					310:325	favorable biocompatibility	300:325	favorable biocompatibility	300:325	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	14	61	theme	cell	2182:2185	arg1	attachment					2187:2196	suitable cell attachment	2173:2196	suitable cell attachment	2173:2196	It revealed suitable cell attachment and proliferation of cells compared to the Ch-G-NBG scaffold.					
36113451	18	62	theme	tissue	2781:2786	arg1	restoration					2788:2798	bone tissue restoration	2776:2798	bone tissue restoration	2776:2798	Based on these results, the nano-biocomposite scaffold appears to have the potential for utilization in bone tissue restoration, replacement and regeneration.					
36113451	2	63	theme	biocompatible	359:371	arg1	chitosan/gelatin/nanobioglass/GO					393:424	chitosan/gelatin/nanobioglass/GO	393:424	chitosan/gelatin/nanobioglass/GO	393:424	In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties.					
36113451	2	63	theme	biocompatible	359:371	arg1	scaffolds					382:390	nanocomposite biocompatible GO-based scaffolds	345:390	nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO	345:437	In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties.					
36113451	1	64	theme	excellent	234:242	arg1	conductivity					255:266	excellent electrical conductivity	234:266	excellent electrical conductivity	234:266	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	3	65	theme	scanning	744:751	arg1	diffraction					709:719	x-ray diffraction	703:719	x-ray diffraction (XRD)	703:725	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	65	theme	scanning	744:751	arg1	microscope					762:771	high resolution scanning electron microscope	728:771	high resolution scanning electron microscope	728:771	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	66	theme	Ch-G-NBG-GO	592:602	arg1	composites					604:613	The prepared Ch-G-NBG-GO composites	579:613	The prepared Ch-G-NBG-GO composites	579:613	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	4	67	theme	morphological	949:961	arg1	analysis					963:970	The morphological analysis	945:970	The morphological analysis	945:970	The morphological analysis showed the porous interconnected network of scaffold formed.					
36113451	3	68	theme	infrared	792:799	arg1	spectroscopy					801:812	infrared spectroscopy	792:812	infrared spectroscopy	792:812	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	12	69	theme	incorporated	1943:1954	arg1	scaffolds					1956:1964	incorporated scaffolds	1943:1964	GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend	1940:2037	DSC and TGA data revealed the thermal stability of GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend.					
36113451	9	70	theme	nanoparticles	1655:1667	arg1	addition					1640:1647	addition	1640:1647	addition of GO nanoparticles	1640:1667	The crystallinity added to the scaffolds due to addition of GO nanoparticles improved the mechanical strength of these scaffolds.					
36113451	7	71	theme	scaffolds	1286:1294	arg1	Porosity					1259:1266	Porosity	1259:1266	Porosity of the fabricated scaffolds	1259:1294	Porosity of the fabricated scaffolds were in the range between 75.3% and 77.3% which was very close to the control scaffold with 79% porosity.					
36113451	1	72	theme	biomedical	158:167	arg1	engineering					169:179	biomedical engineering	158:179	biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility	158:325	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	12	73	with	interaction	1984:1994	arg1	blend					2033:2037	chitosan-gelatin blend	2016:2037	chitosan-gelatin blend	2016:2037	DSC and TGA data revealed the thermal stability of GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend.					
36113451	12	74	theme	thermal	1919:1925	arg1	stability					1927:1935	the thermal stability	1915:1935	the thermal stability of GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend	1915:2037	DSC and TGA data revealed the thermal stability of GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend.					
36113451	6	75	theme	NBG	1209:1211	arg1	incorporation					1192:1204	the successful incorporation	1177:1204	the successful incorporation of NBG	1177:1211	XRD data revealed the successful incorporation of NBG and GO and distribution across the scaffolds.					
36113451	6	75	theme	NBG	1209:1211	arg1	distribution					1224:1235	distribution	1224:1235	distribution	1224:1235	XRD data revealed the successful incorporation of NBG and GO and distribution across the scaffolds.					
36113451	13	76	theme	tissue	2074:2079	arg1	engineering					2081:2091	bone tissue engineering	2069:2091	bone tissue engineering	2069:2091	The scaffold's potential for bone tissue engineering was evaluated by testing its cytocompatibility for MG-63 cell line.					
36113451	12	77	theme	DSC	1889:1891	arg1	data					1901:1904	DSC and TGA data	1889:1904	data	1901:1904	DSC and TGA data revealed the thermal stability of GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend.					
36113451	4	78	theme	scaffold	1016:1023	arg1	network					1005:1011	the porous interconnected network	979:1011	the porous interconnected network of scaffold formed	979:1030	The morphological analysis showed the porous interconnected network of scaffold formed.					
36113451	17	79	theme	based	2571:2575	arg1	scaffolds					2577:2585	based scaffolds	2571:2585	based scaffolds	2571:2585	Alkaline phosphatase activity suggested improved osteogenic differentiation of MG-63 cells over GO based scaffolds and this was due to the osteogenic potential of NBG and GO present in the scaffolds.					
36113451	12	80	theme	TGA	1897:1899	arg1	data					1901:1904	DSC and TGA data	1889:1904	data	1901:1904	DSC and TGA data revealed the thermal stability of GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend.					
36113451	17	81	theme	osteogenic	2611:2620	arg1	potential					2622:2630	the osteogenic potential	2607:2630	the osteogenic potential of NBG	2607:2637	Alkaline phosphatase activity suggested improved osteogenic differentiation of MG-63 cells over GO based scaffolds and this was due to the osteogenic potential of NBG and GO present in the scaffolds.					
36113451	12	82	theme	chitosan-gelatin	2016:2031	arg1	blend					2033:2037	chitosan-gelatin blend	2016:2037	chitosan-gelatin blend	2016:2037	DSC and TGA data revealed the thermal stability of GO incorporated scaffolds due to the proper interaction between GO/NBG with chitosan-gelatin blend.					
36113451	11	83	theme	Swelling	1796:1803	arg1	ratio					1805:1809	Swelling ratio	1796:1809	Swelling ratio	1796:1809	Swelling ratio changed drastically especially in case of Ch-NBG-90%GO (4.9 ± 0.04-4 ± 0.01).					
36113451	3	84	theme	surface	680:686	arg1	morphology					688:697	surface morphology	680:697	surface morphology	680:697	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	17	85	from	present	2646:2652	arg1	scaffolds					2661:2669	the scaffolds	2657:2669	the scaffolds	2657:2669	Alkaline phosphatase activity suggested improved osteogenic differentiation of MG-63 cells over GO based scaffolds and this was due to the osteogenic potential of NBG and GO present in the scaffolds.					
36113451	4	86	theme	porous	983:988	arg1	network					1005:1011	the porous interconnected network	979:1011	the porous interconnected network of scaffold formed	979:1030	The morphological analysis showed the porous interconnected network of scaffold formed.					
36113451	10	87	dep	8.51	1767:1770	arg1	to					1764:1765	to	1764:1765	to	1764:1765	The compressive modulus changed from (5.7 to 8.51) MPa after GO addition.					
36113451	0	88	theme	bone	66:69	arg1	regeneration					78:89	bone tissue regeneration	66:89	bone tissue regeneration	66:89	Generation of graphene oxide and nano-bioglass based scaffold for bone tissue regeneration.					
36113451	1	89	theme	distinct	121:128	arg1	opportunity					130:140	a distinct opportunity	119:140	a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility	119:325	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	7	90	theme	79	1388:1389	arg1	%					1390:1390	%	1390:1390	%	1390:1390	Porosity of the fabricated scaffolds were in the range between 75.3% and 77.3% which was very close to the control scaffold with 79% porosity.					
36113451	3	91	theme	x-ray	867:871	arg1	Spectroscopy					873:884	energy-dispersive x-ray Spectroscopy	849:884	energy-dispersive x-ray Spectroscopy	849:884	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	7	92	theme	fabricated	1275:1284	arg1	scaffolds					1286:1294	the fabricated scaffolds	1271:1294	the fabricated scaffolds	1271:1294	Porosity of the fabricated scaffolds were in the range between 75.3% and 77.3% which was very close to the control scaffold with 79% porosity.					
36113451	17	93	theme	improved	2512:2519	arg1	differentiation					2532:2546	improved osteogenic differentiation	2512:2546	improved osteogenic differentiation of MG-63 cells	2512:2561	Alkaline phosphatase activity suggested improved osteogenic differentiation of MG-63 cells over GO based scaffolds and this was due to the osteogenic potential of NBG and GO present in the scaffolds.					
36113451	3	94	theme	differential	891:902	arg1	DSC					926:928	DSC	926:928	DSC	926:928	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	94	theme	differential	891:902	arg1	colorimetry					913:923	differential scanning colorimetry	891:923	differential scanning colorimetry (DSC)	891:929	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	1	95	from	opportunity	130:140	arg1	field					149:153	the field	145:153	the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility	145:325	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	5	96	theme	Ch-G-NBG-GO	1059:1069	arg1	scaffolds					1071:1079	the Ch-G-NBG-GO scaffolds	1055:1079	the Ch-G-NBG-GO scaffolds	1055:1079	Average pore size for the Ch-G-NBG-GO scaffolds were in between 90 and 120 μm, which was very close to the control scaffolds.					
36113451	7	97	theme	control	1366:1372	arg1	%					1336:1336	77.3%	1332:1336	77.3%	1332:1336	Porosity of the fabricated scaffolds were in the range between 75.3% and 77.3% which was very close to the control scaffold with 79% porosity.					
36113451	7	97	theme	control	1366:1372	arg1	%					1326:1326	75.3%	1322:1326	75.3%	1322:1326	Porosity of the fabricated scaffolds were in the range between 75.3% and 77.3% which was very close to the control scaffold with 79% porosity.					
36113451	7	97	theme	control	1366:1372	arg1	scaffold					1374:1381	the control scaffold	1362:1381	the control scaffold with 79% porosity	1362:1399	Porosity of the fabricated scaffolds were in the range between 75.3% and 77.3% which was very close to the control scaffold with 79% porosity.					
36113451	8	98	theme	room	1539:1542	arg1	temperature					1544:1554	room temperature	1539:1554	room temperature	1539:1554	The studies also reveal that after GO incorporation, the weight loss reduced (0.11 ± 0.02-0.095 ± 0.03), scaffolds were firmly stable at room temperature even after a long duration of 28 d.					
36113451	17	99	theme	MG-63	2551:2555	arg1	cells					2557:2561	MG-63 cells	2551:2561	MG-63 cells	2551:2561	Alkaline phosphatase activity suggested improved osteogenic differentiation of MG-63 cells over GO based scaffolds and this was due to the osteogenic potential of NBG and GO present in the scaffolds.					
36113451	11	100	theme	0.04-4	1873:1878	arg1	%					1862:1862	Ch-NBG-90%GO	1853:1864	Ch-NBG-90%GO (4.9 ± 0.04-4 ± 0.01)	1853:1886	Swelling ratio changed drastically especially in case of Ch-NBG-90%GO (4.9 ± 0.04-4 ± 0.01).					
36113451	11	100	theme	0.04-4	1873:1878	arg1	±					1880:1880	4.9 ± 0.04-4 ± 0.01	1867:1885	4.9 ± 0.04-4 ± 0.01	1867:1885	Swelling ratio changed drastically especially in case of Ch-NBG-90%GO (4.9 ± 0.04-4 ± 0.01).					
36113451	0	101	theme	oxide	23:27	arg1	Generation					0:9	Generation	0:9	Generation of graphene oxide and nano-bioglass	0:45	Generation of graphene oxide and nano-bioglass based scaffold for bone tissue regeneration.					
36113451	1	102	theme	optical	274:280	arg1	transparency					282:293	high optical transparency	269:293	high optical transparency	269:293	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	5	103	theme	pore	1041:1044	arg1	size					1046:1049	Average pore size	1033:1049	Average pore size for the Ch-G-NBG-GO scaffolds	1033:1079	Average pore size for the Ch-G-NBG-GO scaffolds were in between 90 and 120 μm, which was very close to the control scaffolds.					
36113451	2	104	theme	freeze	476:481	arg1	technique					490:498	freeze drying technique	476:498	freeze drying technique (-40 °C)	476:507	In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties.					
36113451	2	104	theme	freeze	476:481	arg1	°C					505:506	-40 °C	501:506	-40 °C	501:506	In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties.					
36113451	10	105	theme	compressive	1726:1736	arg1	modulus					1738:1744	The compressive modulus	1722:1744	The compressive modulus	1722:1744	The compressive modulus changed from (5.7 to 8.51) MPa after GO addition.					
36113451	0	106	theme	nano-bioglass	33:45	arg1	Generation					0:9	Generation	0:9	Generation of graphene oxide and nano-bioglass	0:45	Generation of graphene oxide and nano-bioglass based scaffold for bone tissue regeneration.					
36113451	18	107	theme	bone	2776:2779	arg1	restoration					2788:2798	bone tissue restoration	2776:2798	bone tissue restoration	2776:2798	Based on these results, the nano-biocomposite scaffold appears to have the potential for utilization in bone tissue restoration, replacement and regeneration.					
36113451	3	108	dep	Fourier	774:780	arg1	transform					782:790	transform	782:790	transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively	782:942	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	17	109	theme	NBG	2635:2637	arg1	potential					2622:2630	the osteogenic potential	2607:2630	the osteogenic potential of NBG	2607:2637	Alkaline phosphatase activity suggested improved osteogenic differentiation of MG-63 cells over GO based scaffolds and this was due to the osteogenic potential of NBG and GO present in the scaffolds.					
36113451	6	110	theme	successful	1181:1190	arg1	incorporation					1192:1204	the successful incorporation	1177:1204	the successful incorporation of NBG	1177:1211	XRD data revealed the successful incorporation of NBG and GO and distribution across the scaffolds.					
36113451	16	111	theme	%	2387:2387	arg1	scaffold					2391:2398	Ch-G-NBG-90%GO scaffold	2376:2398	Ch-G-NBG-90%GO scaffold	2376:2398	But in case of Ch-G-NBG-90%GO scaffold, the cell proliferation was reduced when compared to control scaffolds.					
36113451	1	112	theme	mechanical	213:222	arg1	strength					224:231	its exceptionally high mechanical strength	190:231	its exceptionally high mechanical strength	190:231	Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility.					
36113451	3	113	theme	electron	753:760	arg1	diffraction					709:719	x-ray diffraction	703:719	x-ray diffraction (XRD)	703:725	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	3	113	theme	electron	753:760	arg1	microscope					762:771	high resolution scanning electron microscope	728:771	high resolution scanning electron microscope	728:771	The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.					
36113451	15	114	theme	Ch-G-NBG-GO	2282:2292	arg1	scaffold					2294:2301	Ch-G-NBG-GO scaffold	2282:2301	Ch-G-NBG-GO scaffold below 90% GO concentration	2282:2328	MTT assay showed that Ch-G-NBG-GO scaffold below 90% GO concentration possess best biocompatibility.					
36113451	2	115	theme	GO-based	373:380	arg1	chitosan/gelatin/nanobioglass/GO					393:424	chitosan/gelatin/nanobioglass/GO	393:424	chitosan/gelatin/nanobioglass/GO	393:424	In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties.					
36113451	2	115	theme	GO-based	373:380	arg1	scaffolds					382:390	nanocomposite biocompatible GO-based scaffolds	345:390	nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO	345:437	In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties.					
36113451	13	116	theme	cell	2150:2153	arg1	line					2155:2158	MG-63 cell line	2144:2158	MG-63 cell line	2144:2158	The scaffold's potential for bone tissue engineering was evaluated by testing its cytocompatibility for MG-63 cell line.					
36270228	0	0	theme	pH-driven	118:126	arg1	methods					128:134	antisolvent and pH-driven methods	102:134	antisolvent and pH-driven methods for curcumin delivery	102:156	Fabrication of foxtail millet prolamin/caseinate/chitosan hydrochloride composite nanoparticles using antisolvent and pH-driven methods for curcumin delivery.					
36270228	2	1	theme	FP/NaCas	492:499	arg1	nanoparticles					501:513	the two different FP/NaCas nanoparticles	474:513	the two different FP/NaCas nanoparticles (NPs)	474:519	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	2	1	theme	FP/NaCas	492:499	arg1	NPs					516:518	NPs	516:518	NPs	516:518	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	2	2	theme	chitosan	367:374	arg1	CHC					391:393	CHC	391:393	CHC	391:393	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	2	2	theme	chitosan	367:374	arg1	hydrochloride					376:388	chitosan hydrochloride	367:388	chitosan hydrochloride (CHC)	367:394	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	2	3	theme	different	482:490	arg1	nanoparticles					501:513	the two different FP/NaCas nanoparticles	474:513	the two different FP/NaCas nanoparticles (NPs)	474:519	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	2	3	theme	different	482:490	arg1	NPs					516:518	NPs	516:518	NPs	516:518	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	1	4	theme	-sodium	191:197	arg1	nanocomplex					214:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	5	5	theme	coated	1090:1095	arg1	NPs					1100:1102	NaCas coated FP NPs	1084:1102	NaCas coated FP NPs in terms of long-term storage stability	1084:1142	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	5	6	from	NPs	1100:1102	arg1	terms					1107:1111	terms	1107:1111	terms of long-term storage stability	1107:1142	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	4	7	theme	NPs	786:788	arg1	kinds					757:761	the two kinds	749:761	the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths	749:839	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths was different due to their different NP structures.					
36270228	5	8	theme	retention	1050:1058	arg1	rate					1060:1063	higher curcumin retention rate	1034:1063	higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability	1034:1142	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	4	9	theme	kinds	757:761	arg1	stability					736:744	The stability	732:744	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths	732:839	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths was different due to their different NP structures.					
36270228	4	9	theme	kinds	757:761	arg1	different					845:853	different	845:853	different	845:853	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths was different due to their different NP structures.					
36270228	5	10	from	curcumin	971:978	arg1	microenvironment					1006:1021	relatively hydrophilic microenvironment	983:1021	relatively hydrophilic microenvironment	983:1021	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	6	11	theme	FP/NaCas	1195:1202	arg1	beneficial					1232:1241	beneficial	1232:1241	beneficial	1232:1241	The results revealed that the produced CHC coated FP/NaCas nanocomplexes could be very beneficial in entrapping and delivering bioactive substances.					
36270228	6	11	theme	FP/NaCas	1195:1202	arg1	nanocomplexes					1204:1216	the produced CHC coated FP/NaCas nanocomplexes	1171:1216	the produced CHC coated FP/NaCas nanocomplexes	1171:1216	The results revealed that the produced CHC coated FP/NaCas nanocomplexes could be very beneficial in entrapping and delivering bioactive substances.					
36270228	2	12	from	effects	356:362	arg1	size					420:423	particle size	411:423	particle size	411:423	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	2	12	from	effects	356:362	arg1	charge					434:439	surface charge	426:439	surface charge	426:439	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	2	12	from	effects	356:362	arg1	stability					461:469	physicochemical stability	445:469	physicochemical stability	445:469	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	5	13	theme	hydrophilic	994:1004	arg1	microenvironment					1006:1021	relatively hydrophilic microenvironment	983:1021	relatively hydrophilic microenvironment	983:1021	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	5	14	with	comparison	1068:1077	arg1	NPs					1100:1102	NaCas coated FP NPs	1084:1102	NaCas coated FP NPs in terms of long-term storage stability	1084:1142	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	4	15	theme	ionic	825:829	arg1	strengths					831:839	varying ionic strengths	817:839	varying ionic strengths	817:839	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths was different due to their different NP structures.					
36270228	4	16	theme	CHC	766:768	arg1	NPs					786:788	CHC coated FP-based NPs	766:788	CHC coated FP-based NPs to different pH values and varying ionic strengths	766:839	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths was different due to their different NP structures.					
36270228	1	17	theme	casein	199:204	arg1	nanocomplex					214:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	0	18	theme	curcumin	140:147	arg1	delivery					149:156	curcumin delivery	140:156	curcumin delivery	140:156	Fabrication of foxtail millet prolamin/caseinate/chitosan hydrochloride composite nanoparticles using antisolvent and pH-driven methods for curcumin delivery.					
36270228	5	19	theme	long-term	1116:1124	arg1	stability					1134:1142	long-term storage stability	1116:1142	long-term storage stability	1116:1142	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	4	20	theme	varying	817:823	arg1	strengths					831:839	varying ionic strengths	817:839	varying ionic strengths	817:839	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths was different due to their different NP structures.					
36270228	6	21	theme	CHC	1184:1186	arg1	beneficial					1232:1241	beneficial	1232:1241	beneficial	1232:1241	The results revealed that the produced CHC coated FP/NaCas nanocomplexes could be very beneficial in entrapping and delivering bioactive substances.					
36270228	6	21	theme	CHC	1184:1186	arg1	nanocomplexes					1204:1216	the produced CHC coated FP/NaCas nanocomplexes	1171:1216	the produced CHC coated FP/NaCas nanocomplexes	1171:1216	The results revealed that the produced CHC coated FP/NaCas nanocomplexes could be very beneficial in entrapping and delivering bioactive substances.					
36270228	2	22	theme	physicochemical	445:459	arg1	stability					461:469	physicochemical stability	445:469	physicochemical stability	445:469	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	5	23	theme	storage	1126:1132	arg1	stability					1134:1142	long-term storage stability	1116:1142	long-term storage stability	1116:1142	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	6	24	theme	produced	1175:1182	arg1	beneficial					1232:1241	beneficial	1232:1241	beneficial	1232:1241	The results revealed that the produced CHC coated FP/NaCas nanocomplexes could be very beneficial in entrapping and delivering bioactive substances.					
36270228	6	24	theme	produced	1175:1182	arg1	nanocomplexes					1204:1216	the produced CHC coated FP/NaCas nanocomplexes	1171:1216	the produced CHC coated FP/NaCas nanocomplexes	1171:1216	The results revealed that the produced CHC coated FP/NaCas nanocomplexes could be very beneficial in entrapping and delivering bioactive substances.					
36270228	5	25	theme	different	899:907	arg1	formulations					921:932	different fabrication formulations	899:932	different fabrication formulations	899:932	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	1	26	theme	NaCas	207:211	arg1	nanocomplex					214:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	6	27	theme	bioactive	1272:1280	arg1	substances					1282:1291	bioactive substances	1272:1291	bioactive substances	1272:1291	The results revealed that the produced CHC coated FP/NaCas nanocomplexes could be very beneficial in entrapping and delivering bioactive substances.					
36270228	5	28	theme	co-assembled	935:946	arg1	NPs					957:959	co-assembled FP-NaCas NPs	935:959	co-assembled FP-NaCas NPs	935:959	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	5	29	theme	FP	1097:1098	arg1	NPs					1100:1102	NaCas coated FP NPs	1084:1102	NaCas coated FP NPs in terms of long-term storage stability	1084:1142	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	0	30	theme	millet	23:28	arg1	composite					72:80	foxtail millet prolamin/caseinate/chitosan hydrochloride composite	15:80	foxtail millet prolamin/caseinate/chitosan hydrochloride composite	15:80	Fabrication of foxtail millet prolamin/caseinate/chitosan hydrochloride composite nanoparticles using antisolvent and pH-driven methods for curcumin delivery.					
36270228	1	31	theme	pH-cycle	289:296	arg1	methods					315:321	pH-cycle and anti-solvent methods	289:321	pH-cycle and anti-solvent methods	289:321	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	5	32	theme	FP-NaCas	948:955	arg1	NPs					957:959	co-assembled FP-NaCas NPs	935:959	co-assembled FP-NaCas NPs	935:959	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	5	33	theme	higher	1034:1039	arg1	rate					1060:1063	higher curcumin retention rate	1034:1063	higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability	1034:1142	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	1	34	theme	nanocomplex	214:224	arg1	NPs					261:263	NPs	261:263	NPs	261:263	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	1	34	theme	nanocomplex	214:224	arg1	nanoparticles					246:258	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles	159:258	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs)	159:264	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	0	35	theme	foxtail	15:21	arg1	composite					72:80	foxtail millet prolamin/caseinate/chitosan hydrochloride composite	15:80	foxtail millet prolamin/caseinate/chitosan hydrochloride composite	15:80	Fabrication of foxtail millet prolamin/caseinate/chitosan hydrochloride composite nanoparticles using antisolvent and pH-driven methods for curcumin delivery.					
36270228	6	36	theme	coated	1188:1193	arg1	beneficial					1232:1241	beneficial	1232:1241	beneficial	1232:1241	The results revealed that the produced CHC coated FP/NaCas nanocomplexes could be very beneficial in entrapping and delivering bioactive substances.					
36270228	6	36	theme	coated	1188:1193	arg1	nanocomplexes					1204:1216	the produced CHC coated FP/NaCas nanocomplexes	1171:1216	the produced CHC coated FP/NaCas nanocomplexes	1171:1216	The results revealed that the produced CHC coated FP/NaCas nanocomplexes could be very beneficial in entrapping and delivering bioactive substances.					
36270228	3	37	theme	nanocomplex	630:640	arg1	size					576:579	the particle size	563:579	the particle size of NaCas coated FP NPs and co-assembled FP-NaCas nanocomplex	563:640	With the addition of CHC, the particle size of NaCas coated FP NPs and co-assembled FP-NaCas nanocomplex significantly increased from 128.3 nm and 69.5 nm to 183.5 nm and 113.8 nm, respectively.					
36270228	3	38	theme	CHC	558:560	arg1	addition					546:553	the addition	542:553	the addition of CHC	542:560	With the addition of CHC, the particle size of NaCas coated FP NPs and co-assembled FP-NaCas nanocomplex significantly increased from 128.3 nm and 69.5 nm to 183.5 nm and 113.8 nm, respectively.					
36270228	0	39	theme	hydrochloride	58:70	arg1	composite					72:80	foxtail millet prolamin/caseinate/chitosan hydrochloride composite	15:80	foxtail millet prolamin/caseinate/chitosan hydrochloride composite	15:80	Fabrication of foxtail millet prolamin/caseinate/chitosan hydrochloride composite nanoparticles using antisolvent and pH-driven methods for curcumin delivery.					
36270228	1	40	theme	anti-solvent	302:313	arg1	methods					315:321	pH-cycle and anti-solvent methods	289:321	pH-cycle and anti-solvent methods	289:321	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	1	41	theme	NaCas	230:234	arg1	FP					243:244	NaCas coated FP	230:244	NaCas coated FP	230:244	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	4	42	theme	pH	803:804	arg1	values					806:811	different pH values	793:811	different pH values	793:811	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths was different due to their different NP structures.					
36270228	5	43	from	rate	1060:1063	arg1	comparison					1068:1077	comparison	1068:1077	comparison with NaCas coated FP NPs in terms of long-term storage stability	1068:1142	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	2	44	theme	particle	411:418	arg1	size					420:423	particle size	411:423	particle size	411:423	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	0	45	theme	prolamin/caseinate/chitosan	30:56	arg1	composite					72:80	foxtail millet prolamin/caseinate/chitosan hydrochloride composite	15:80	foxtail millet prolamin/caseinate/chitosan hydrochloride composite	15:80	Fabrication of foxtail millet prolamin/caseinate/chitosan hydrochloride composite nanoparticles using antisolvent and pH-driven methods for curcumin delivery.					
36270228	3	46	theme	co-assembled	608:619	arg1	nanocomplex					630:640	co-assembled FP-NaCas nanocomplex	608:640	co-assembled FP-NaCas nanocomplex	608:640	With the addition of CHC, the particle size of NaCas coated FP NPs and co-assembled FP-NaCas nanocomplex significantly increased from 128.3 nm and 69.5 nm to 183.5 nm and 113.8 nm, respectively.					
36270228	3	47	theme	NaCas	584:588	arg1	NPs					600:602	NaCas coated FP NPs	584:602	NaCas coated FP NPs	584:602	With the addition of CHC, the particle size of NaCas coated FP NPs and co-assembled FP-NaCas nanocomplex significantly increased from 128.3 nm and 69.5 nm to 183.5 nm and 113.8 nm, respectively.					
36270228	1	48	theme	coated	236:241	arg1	FP					243:244	NaCas coated FP	230:244	NaCas coated FP	230:244	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	4	49	theme	NP	878:879	arg1	structures					881:890	their different NP structures	862:890	their different NP structures	862:890	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths was different due to their different NP structures.					
36270228	3	50	theme	FP-NaCas	621:628	arg1	nanocomplex					630:640	co-assembled FP-NaCas nanocomplex	608:640	co-assembled FP-NaCas nanocomplex	608:640	With the addition of CHC, the particle size of NaCas coated FP NPs and co-assembled FP-NaCas nanocomplex significantly increased from 128.3 nm and 69.5 nm to 183.5 nm and 113.8 nm, respectively.					
36270228	3	51	theme	particle	567:574	arg1	size					576:579	the particle size	563:579	the particle size of NaCas coated FP NPs and co-assembled FP-NaCas nanocomplex	563:640	With the addition of CHC, the particle size of NaCas coated FP NPs and co-assembled FP-NaCas nanocomplex significantly increased from 128.3 nm and 69.5 nm to 183.5 nm and 113.8 nm, respectively.					
36270228	0	52	theme	composite	72:80	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of foxtail millet prolamin/caseinate/chitosan hydrochloride composite	0:80	Fabrication of foxtail millet prolamin/caseinate/chitosan hydrochloride composite nanoparticles using antisolvent and pH-driven methods for curcumin delivery.					
36270228	4	53	theme	different	868:876	arg1	structures					881:890	their different NP structures	862:890	their different NP structures	862:890	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths was different due to their different NP structures.					
36270228	2	54	dep	size	420:423	arg1	the					407:409	the	407:409	the	407:409	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	1	55	theme	Co-assembled	159:170	arg1	nanocomplex					214:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	2	56	theme	hydrochloride	376:388	arg1	effects					356:362	the effects	352:362	the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs)	352:519	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	3	57	theme	FP	597:598	arg1	NPs					600:602	NaCas coated FP NPs	584:602	NaCas coated FP NPs	584:602	With the addition of CHC, the particle size of NaCas coated FP NPs and co-assembled FP-NaCas nanocomplex significantly increased from 128.3 nm and 69.5 nm to 183.5 nm and 113.8 nm, respectively.					
36270228	5	58	theme	curcumin	1041:1048	arg1	rate					1060:1063	higher curcumin retention rate	1034:1063	higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability	1034:1142	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	4	59	theme	FP-based	777:784	arg1	NPs					786:788	CHC coated FP-based NPs	766:788	CHC coated FP-based NPs to different pH values and varying ionic strengths	766:839	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths was different due to their different NP structures.					
36270228	3	60	theme	NPs	600:602	arg1	size					576:579	the particle size	563:579	the particle size of NaCas coated FP NPs and co-assembled FP-NaCas nanocomplex	563:640	With the addition of CHC, the particle size of NaCas coated FP NPs and co-assembled FP-NaCas nanocomplex significantly increased from 128.3 nm and 69.5 nm to 183.5 nm and 113.8 nm, respectively.					
36270228	4	61	theme	different	793:801	arg1	values					806:811	different pH values	793:811	different pH values	793:811	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths was different due to their different NP structures.					
36270228	1	62	theme	foxtail	172:178	arg1	nanocomplex					214:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	1	63	theme	FP	243:244	arg1	NPs					261:263	NPs	261:263	NPs	261:263	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	1	63	theme	FP	243:244	arg1	nanoparticles					246:258	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles	159:258	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs)	159:264	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	4	64	theme	coated	770:775	arg1	NPs					786:788	CHC coated FP-based NPs	766:788	CHC coated FP-based NPs to different pH values and varying ionic strengths	766:839	The stability of the two kinds of CHC coated FP-based NPs to different pH values and varying ionic strengths was different due to their different NP structures.					
36270228	1	65	theme	millet	180:185	arg1	nanocomplex					214:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
36270228	5	66	theme	fabrication	909:919	arg1	formulations					921:932	different fabrication formulations	899:932	different fabrication formulations	899:932	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	0	67	theme	antisolvent	102:112	arg1	methods					128:134	antisolvent and pH-driven methods	102:134	antisolvent and pH-driven methods for curcumin delivery	102:156	Fabrication of foxtail millet prolamin/caseinate/chitosan hydrochloride composite nanoparticles using antisolvent and pH-driven methods for curcumin delivery.					
36270228	5	68	theme	NaCas	1084:1088	arg1	NPs					1100:1102	NaCas coated FP NPs	1084:1102	NaCas coated FP NPs in terms of long-term storage stability	1084:1142	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	3	69	theme	coated	590:595	arg1	NPs					600:602	NaCas coated FP NPs	584:602	NaCas coated FP NPs	584:602	With the addition of CHC, the particle size of NaCas coated FP NPs and co-assembled FP-NaCas nanocomplex significantly increased from 128.3 nm and 69.5 nm to 183.5 nm and 113.8 nm, respectively.					
36270228	2	70	theme	nanoparticles	501:513	arg1	size					420:423	particle size	411:423	particle size	411:423	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	2	70	theme	nanoparticles	501:513	arg1	charge					434:439	surface charge	426:439	surface charge	426:439	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	2	70	theme	nanoparticles	501:513	arg1	stability					461:469	physicochemical stability	445:469	physicochemical stability	445:469	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	5	71	theme	stability	1134:1142	arg1	terms					1107:1111	terms	1107:1111	terms of long-term storage stability	1107:1142	Using different fabrication formulations, co-assembled FP-NaCas NPs entrapped curcumin in relatively hydrophilic microenvironment and showed higher curcumin retention rate in comparison with NaCas coated FP NPs in terms of long-term storage stability.					
36270228	2	72	theme	surface	426:432	arg1	charge					434:439	surface charge	426:439	surface charge	426:439	Subsequently, the effects of chitosan hydrochloride (CHC) coating on the particle size, surface charge and physicochemical stability of the two different FP/NaCas nanoparticles (NPs) were evaluated.					
36270228	1	73	theme	FP	188:189	arg1	nanocomplex					214:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex	159:224	Co-assembled foxtail millet (FP)-sodium casein (NaCas) nanocomplex and NaCas coated FP nanoparticles (NPs) were produced by using pH-cycle and anti-solvent methods, respectively.					
37203871	9	0	theme	epigastric	1631:1640	arg1	pain					1642:1645	epigastric pain	1631:1645	epigastric pain	1631:1645	RIF also significantly improved gastric fullness, epigastric pain and heartburn.					
37203871	5	1	from	decrease	1126:1133	arg1	thickness					1168:1176	subcutaneous and visceral fat thickness	1138:1176	subcutaneous and visceral fat thickness	1138:1176	Although physical activity remained stable before, during, and after RIF, body weight, body mass index and waist circumference decreased in all subjects and in both genders, together with a significant decrease in subcutaneous and visceral fat thickness and insulin resistance.					
37203871	5	1	from	decrease	1126:1133	arg1	resistance					1190:1199	insulin resistance	1182:1199	insulin resistance	1182:1199	Although physical activity remained stable before, during, and after RIF, body weight, body mass index and waist circumference decreased in all subjects and in both genders, together with a significant decrease in subcutaneous and visceral fat thickness and insulin resistance.					
37203871	11	2	theme	potential	1878:1886	arg1	effects					1899:1905	the potential beneficial effects	1874:1905	the potential beneficial effects of RIF in diseased people	1874:1931	Further comprehensive studies should assess the potential beneficial effects of RIF in diseased people.					
37203871	7	3	theme	postprandial	1393:1404	arg1	contraction					1418:1428	a stronger and faster postprandial gallbladder contraction	1371:1428	a stronger and faster postprandial gallbladder contraction	1371:1428	Fasting gallbladder volume was about 6% smaller after, than before Ramadan, with a stronger and faster postprandial gallbladder contraction.					
37203871	5	4	theme	insulin	1182:1188	arg1	resistance					1190:1199	insulin resistance	1182:1199	insulin resistance	1182:1199	Although physical activity remained stable before, during, and after RIF, body weight, body mass index and waist circumference decreased in all subjects and in both genders, together with a significant decrease in subcutaneous and visceral fat thickness and insulin resistance.					
37203871	6	5	theme	gastric	1219:1225	arg1	faster					1260:1265	faster	1260:1265	faster	1260:1265	The postprandial gastric emptying speed was significantly faster after than before RIF.					
37203871	6	5	theme	gastric	1219:1225	arg1	emptying					1227:1234	The postprandial gastric emptying	1202:1234	The postprandial gastric emptying speed	1202:1240	The postprandial gastric emptying speed was significantly faster after than before RIF.					
37203871	2	6	theme	metabolic	314:322	arg1	indices					324:330	metabolic indices	314:330	metabolic indices	314:330	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	3	7	theme	breath	598:603	arg1	test					605:608	lactulose breath test	588:608	lactulose breath test	588:608	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	4	8	dep	RESULTS	726:732	arg1	decreased					754:762	decreased	754:762	decreased from a median of 2069 kcal (range 1677-2641) before Ramadan to 1798 kcal (1289-3126) during Ramadan	754:862	RESULTS Mean caloric intake decreased from a median of 2069 kcal (range 1677-2641) before Ramadan to 1798 kcal (1289-3126) during Ramadan and increased again to 2000 kcal (1309-3485) after Ramadan.					
37203871	4	8	dep	RESULTS	726:732	arg1	increased					868:876	increased	868:876	increased again to 2000 kcal (1309-3485) after Ramadan	868:921	RESULTS Mean caloric intake decreased from a median of 2069 kcal (range 1677-2641) before Ramadan to 1798 kcal (1289-3126) during Ramadan and increased again to 2000 kcal (1309-3485) after Ramadan.					
37203871	5	9	theme	waist	1031:1035	arg1	circumference					1037:1049	waist circumference	1031:1049	waist circumference	1031:1049	Although physical activity remained stable before, during, and after RIF, body weight, body mass index and waist circumference decreased in all subjects and in both genders, together with a significant decrease in subcutaneous and visceral fat thickness and insulin resistance.					
37203871	10	10	theme	fat	1759:1761	arg1	burden					1763:1768	fat burden	1759:1768	fat burden	1759:1768	CONCLUSIONS RIF generates, in healthy subjects, multiple systemic beneficial effects in terms of fat burden, metabolic profile, gastrointestinal motility and symptoms.					
37203871	6	11	theme	postprandial	1206:1217	arg1	faster					1260:1265	faster	1260:1265	faster	1260:1265	The postprandial gastric emptying speed was significantly faster after than before RIF.					
37203871	6	11	theme	postprandial	1206:1217	arg1	emptying					1227:1234	The postprandial gastric emptying	1202:1234	The postprandial gastric emptying speed	1202:1240	The postprandial gastric emptying speed was significantly faster after than before RIF.					
37203871	10	12	theme	gastrointestinal	1790:1805	arg1	motility					1807:1814	gastrointestinal motility	1790:1814	gastrointestinal motility	1790:1814	CONCLUSIONS RIF generates, in healthy subjects, multiple systemic beneficial effects in terms of fat burden, metabolic profile, gastrointestinal motility and symptoms.					
37203871	5	13	theme	body	1011:1014	arg1	index					1021:1025	body mass index	1011:1025	body mass index	1011:1025	Although physical activity remained stable before, during, and after RIF, body weight, body mass index and waist circumference decreased in all subjects and in both genders, together with a significant decrease in subcutaneous and visceral fat thickness and insulin resistance.					
37203871	10	14	theme	profile	1781:1787	arg1	terms					1750:1754	terms	1750:1754	terms of fat burden, metabolic profile, gastrointestinal motility and symptoms	1750:1827	CONCLUSIONS RIF generates, in healthy subjects, multiple systemic beneficial effects in terms of fat burden, metabolic profile, gastrointestinal motility and symptoms.					
37203871	1	15	theme	intermittent	123:134	arg1	fasting					136:142	intermittent fasting	123:142	intermittent fasting linked with possible beneficial effects	123:182	BACKGROUND Ramadan is a model of intermittent fasting linked with possible beneficial effects.					
37203871	4	16	theme	Mean	734:737	arg1	intake					747:752	Mean caloric intake	734:752	Mean caloric intake	734:752	RESULTS Mean caloric intake decreased from a median of 2069 kcal (range 1677-2641) before Ramadan to 1798 kcal (1289-3126) during Ramadan and increased again to 2000 kcal (1309-3485) after Ramadan.					
37203871	2	17	theme	intermittent	265:276	arg1	RIF					287:289	RIF	287:289	RIF	287:289	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	2	17	theme	intermittent	265:276	arg1	fasting					278:284	Ramadan intermittent fasting	257:284	Ramadan intermittent fasting (RIF)	257:290	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	8	18	theme	breath	1452:1457	arg1	test					1459:1462	lactulose breath test	1442:1462	lactulose breath test	1442:1462	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	3	19	theme	caloric	438:444	arg1	intake					446:451	caloric intake	438:451	caloric intake	438:451	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	0	20	theme	gastrointestinal	63:78	arg1	motility					80:87	gastrointestinal motility	63:87	gastrointestinal motility	63:87	Ramadan intermittent fasting reduces visceral fat and improves gastrointestinal motility.					
37203871	3	21	theme	anthropometric	612:625	arg1	indices					627:633	anthropometric indices	612:633	anthropometric indices	612:633	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	1	22	theme	possible	156:163	arg1	effects					176:182	possible beneficial effects	156:182	possible beneficial effects	156:182	BACKGROUND Ramadan is a model of intermittent fasting linked with possible beneficial effects.					
37203871	8	23	theme	microbiota	1485:1494	arg1	peak					1539:1542	postprandial H2 peak	1523:1542	postprandial H2 peak	1523:1542	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	8	23	theme	microbiota	1485:1494	arg1	fermentation					1509:1520	increased microbiota carbohydrate fermentation	1475:1520	increased microbiota carbohydrate fermentation (postprandial H2 peak)	1475:1543	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	11	24	from	effects	1899:1905	arg1	people					1926:1931	diseased people	1917:1931	diseased people	1917:1931	Further comprehensive studies should assess the potential beneficial effects of RIF in diseased people.					
37203871	4	25	theme	2069 kcal	781:789	arg1	median					771:776	a median	769:776	a median of 2069 kcal (range 1677-2641) before Ramadan to 1798 kcal (1289-3126)	769:847	RESULTS Mean caloric intake decreased from a median of 2069 kcal (range 1677-2641) before Ramadan to 1798 kcal (1289-3126) during Ramadan and increased again to 2000 kcal (1309-3485) after Ramadan.					
37203871	3	26	theme	RIF	431:433	arg1	impact					421:426	the impact	417:426	the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis	417:723	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	1	27	theme	BACKGROUND	90:99	arg1	Ramadan					101:107	BACKGROUND Ramadan	90:107	BACKGROUND Ramadan	90:107	BACKGROUND Ramadan is a model of intermittent fasting linked with possible beneficial effects.					
37203871	1	27	theme	BACKGROUND	90:99	arg1	model					114:118	a model	112:118	a model of intermittent fasting linked with possible beneficial effects	112:182	BACKGROUND Ramadan is a model of intermittent fasting linked with possible beneficial effects.					
37203871	2	28	from	effects	246:252	arg1	symptoms					350:357	gastrointestinal symptoms	333:357	gastrointestinal symptoms	333:357	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	2	28	from	effects	246:252	arg1	motility					364:371	motility	364:371	motility	364:371	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	2	28	from	effects	246:252	arg1	anthropometric					295:308	anthropometric	295:308	anthropometric	295:308	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	2	28	from	effects	246:252	arg1	indices					324:330	metabolic indices	314:330	metabolic indices	314:330	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	3	29	theme	healthy	388:394	arg1	Muslims					396:402	21 healthy Muslims	385:402	21 healthy Muslims	385:402	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	8	30	theme	postprandial	1523:1534	arg1	peak					1539:1542	postprandial H2 peak	1523:1542	postprandial H2 peak	1523:1542	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	8	30	theme	postprandial	1523:1534	arg1	fermentation					1509:1520	increased microbiota carbohydrate fermentation	1475:1520	increased microbiota carbohydrate fermentation (postprandial H2 peak)	1475:1543	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	0	31	theme	Ramadan	0:6	arg1	fasting					21:27	Ramadan intermittent fasting	0:27	Ramadan intermittent fasting	0:27	Ramadan intermittent fasting reduces visceral fat and improves gastrointestinal motility.					
37203871	5	32	theme	physical	933:940	arg1	activity					942:949	physical activity	933:949	physical activity	933:949	Although physical activity remained stable before, during, and after RIF, body weight, body mass index and waist circumference decreased in all subjects and in both genders, together with a significant decrease in subcutaneous and visceral fat thickness and insulin resistance.					
37203871	2	33	theme	Ramadan	257:263	arg1	RIF					287:289	RIF	287:289	RIF	287:289	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	2	33	theme	Ramadan	257:263	arg1	fasting					278:284	Ramadan intermittent fasting	257:284	Ramadan intermittent fasting (RIF)	257:290	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	10	34	theme	beneficial	1728:1737	arg1	effects					1739:1745	multiple systemic beneficial effects	1710:1745	multiple systemic beneficial effects	1710:1745	CONCLUSIONS RIF generates, in healthy subjects, multiple systemic beneficial effects in terms of fat burden, metabolic profile, gastrointestinal motility and symptoms.					
37203871	7	35	theme	Fasting	1290:1296	arg1	volume					1310:1315	Fasting gallbladder volume	1290:1315	Fasting gallbladder volume	1290:1315	Fasting gallbladder volume was about 6% smaller after, than before Ramadan, with a stronger and faster postprandial gallbladder contraction.					
37203871	3	36	theme	fat	662:664	arg1	ultrasonography					677:691	ultrasonography	677:691	ultrasonography	677:691	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	36	theme	fat	662:664	arg1	thickness					666:674	subcutaneous and visceral fat thickness	636:674	subcutaneous and visceral fat thickness (ultrasonography)	636:692	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	10	37	theme	CONCLUSIONS	1662:1672	arg1	RIF					1674:1676	CONCLUSIONS RIF	1662:1676	CONCLUSIONS RIF	1662:1676	CONCLUSIONS RIF generates, in healthy subjects, multiple systemic beneficial effects in terms of fat burden, metabolic profile, gastrointestinal motility and symptoms.					
37203871	0	38	theme	visceral	37:44	arg1	fat					46:48	visceral fat	37:48	visceral fat	37:48	Ramadan intermittent fasting reduces visceral fat and improves gastrointestinal motility.					
37203871	5	39	theme	body	998:1001	arg1	weight					1003:1008	body weight	998:1008	body weight	998:1008	Although physical activity remained stable before, during, and after RIF, body weight, body mass index and waist circumference decreased in all subjects and in both genders, together with a significant decrease in subcutaneous and visceral fat thickness and insulin resistance.					
37203871	8	40	theme	faster	1550:1555	arg1	time					1575:1578	faster orocaecal transit time	1550:1578	faster orocaecal transit time	1550:1578	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	3	41	theme	gastrointestinal	473:488	arg1	symptoms					490:497	gastrointestinal symptoms	473:497	gastrointestinal symptoms	473:497	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	5	42	theme	significant	1114:1124	arg1	decrease					1126:1133	a significant decrease	1112:1133	a significant decrease in subcutaneous and visceral fat thickness and insulin resistance	1112:1199	Although physical activity remained stable before, during, and after RIF, body weight, body mass index and waist circumference decreased in all subjects and in both genders, together with a significant decrease in subcutaneous and visceral fat thickness and insulin resistance.					
37203871	2	43	theme	Scarce	185:190	arg1	information					192:202	Scarce information	185:202	Scarce information	185:202	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	8	44	theme	transit	1567:1573	arg1	time					1575:1578	faster orocaecal transit time	1550:1578	faster orocaecal transit time	1550:1578	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	2	45	theme	gastrointestinal	333:348	arg1	symptoms					350:357	gastrointestinal symptoms	333:357	gastrointestinal symptoms	333:357	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	9	46	theme	gastric	1613:1619	arg1	fullness					1621:1628	gastric fullness	1613:1628	gastric fullness	1613:1628	RIF also significantly improved gastric fullness, epigastric pain and heartburn.					
37203871	11	47	theme	beneficial	1888:1897	arg1	effects					1899:1905	the potential beneficial effects	1874:1905	the potential beneficial effects of RIF in diseased people	1874:1931	Further comprehensive studies should assess the potential beneficial effects of RIF in diseased people.					
37203871	3	48	theme	lipid	707:711	arg1	homeostasis					713:723	lipid homeostasis	707:723	lipid homeostasis	707:723	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	10	49	theme	multiple	1710:1717	arg1	effects					1739:1745	multiple systemic beneficial effects	1710:1745	multiple systemic beneficial effects	1710:1745	CONCLUSIONS RIF generates, in healthy subjects, multiple systemic beneficial effects in terms of fat burden, metabolic profile, gastrointestinal motility and symptoms.					
37203871	5	50	theme	fat	1164:1166	arg1	thickness					1168:1176	subcutaneous and visceral fat thickness	1138:1176	subcutaneous and visceral fat thickness	1138:1176	Although physical activity remained stable before, during, and after RIF, body weight, body mass index and waist circumference decreased in all subjects and in both genders, together with a significant decrease in subcutaneous and visceral fat thickness and insulin resistance.					
37203871	7	51	theme	gallbladder	1406:1416	arg1	contraction					1418:1428	a stronger and faster postprandial gallbladder contraction	1371:1428	a stronger and faster postprandial gallbladder contraction	1371:1428	Fasting gallbladder volume was about 6% smaller after, than before Ramadan, with a stronger and faster postprandial gallbladder contraction.					
37203871	7	52	theme	faster	1386:1391	arg1	contraction					1418:1428	a stronger and faster postprandial gallbladder contraction	1371:1428	a stronger and faster postprandial gallbladder contraction	1371:1428	Fasting gallbladder volume was about 6% smaller after, than before Ramadan, with a stronger and faster postprandial gallbladder contraction.					
37203871	10	53	theme	metabolic	1771:1779	arg1	profile					1781:1787	metabolic profile	1771:1787	metabolic profile	1771:1787	CONCLUSIONS RIF generates, in healthy subjects, multiple systemic beneficial effects in terms of fat burden, metabolic profile, gastrointestinal motility and symptoms.					
37203871	7	54	theme	stronger	1373:1380	arg1	contraction					1418:1428	a stronger and faster postprandial gallbladder contraction	1371:1428	a stronger and faster postprandial gallbladder contraction	1371:1428	Fasting gallbladder volume was about 6% smaller after, than before Ramadan, with a stronger and faster postprandial gallbladder contraction.					
37203871	3	55	theme	orocaecal	562:570	arg1	emptying					533:540	gastric/gallbladder emptying	513:540	gastric/gallbladder emptying by ultrasonography	513:559	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	55	theme	orocaecal	562:570	arg1	time					580:583	orocaecal transit time	562:583	orocaecal transit time by lactulose breath test	562:608	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	10	56	theme	motility	1807:1814	arg1	terms					1750:1754	terms	1750:1754	terms of fat burden, metabolic profile, gastrointestinal motility and symptoms	1750:1827	CONCLUSIONS RIF generates, in healthy subjects, multiple systemic beneficial effects in terms of fat burden, metabolic profile, gastrointestinal motility and symptoms.					
37203871	4	57	dep	Ramadan	816:822	arg1	to					824:825	to	824:825	to	824:825	RESULTS Mean caloric intake decreased from a median of 2069 kcal (range 1677-2641) before Ramadan to 1798 kcal (1289-3126) during Ramadan and increased again to 2000 kcal (1309-3485) after Ramadan.					
37203871	4	57	dep	Ramadan	816:822	arg1	1798 kcal					827:835	1798 kcal (1289-3126)	827:847	Ramadan to 1798 kcal (1289-3126)	816:847	RESULTS Mean caloric intake decreased from a median of 2069 kcal (range 1677-2641) before Ramadan to 1798 kcal (1289-3126) during Ramadan and increased again to 2000 kcal (1309-3485) after Ramadan.					
37203871	11	58	theme	Further	1830:1836	arg1	studies					1852:1858	Further comprehensive studies	1830:1858	Further comprehensive studies	1830:1858	Further comprehensive studies should assess the potential beneficial effects of RIF in diseased people.					
37203871	3	59	theme	lactulose	588:596	arg1	test					605:608	lactulose breath test	588:608	lactulose breath test	588:608	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	8	60	theme	lactulose	1442:1450	arg1	test					1459:1462	lactulose breath test	1442:1462	lactulose breath test	1442:1462	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	5	61	theme	mass	1016:1019	arg1	index					1021:1025	body mass index	1011:1025	body mass index	1011:1025	Although physical activity remained stable before, during, and after RIF, body weight, body mass index and waist circumference decreased in all subjects and in both genders, together with a significant decrease in subcutaneous and visceral fat thickness and insulin resistance.					
37203871	2	62	theme	fasting	278:284	arg1	effects					246:252	the combined effects	233:252	the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility	233:371	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	1	63	theme	fasting	136:142	arg1	Ramadan					101:107	BACKGROUND Ramadan	90:107	BACKGROUND Ramadan	90:107	BACKGROUND Ramadan is a model of intermittent fasting linked with possible beneficial effects.					
37203871	1	63	theme	fasting	136:142	arg1	model					114:118	a model	112:118	a model of intermittent fasting linked with possible beneficial effects	112:182	BACKGROUND Ramadan is a model of intermittent fasting linked with possible beneficial effects.					
37203871	10	64	theme	burden	1763:1768	arg1	terms					1750:1754	terms	1750:1754	terms of fat burden, metabolic profile, gastrointestinal motility and symptoms	1750:1827	CONCLUSIONS RIF generates, in healthy subjects, multiple systemic beneficial effects in terms of fat burden, metabolic profile, gastrointestinal motility and symptoms.					
37203871	11	65	theme	comprehensive	1838:1850	arg1	studies					1852:1858	Further comprehensive studies	1830:1858	Further comprehensive studies	1830:1858	Further comprehensive studies should assess the potential beneficial effects of RIF in diseased people.					
37203871	10	66	theme	symptoms	1820:1827	arg1	terms					1750:1754	terms	1750:1754	terms of fat burden, metabolic profile, gastrointestinal motility and symptoms	1750:1827	CONCLUSIONS RIF generates, in healthy subjects, multiple systemic beneficial effects in terms of fat burden, metabolic profile, gastrointestinal motility and symptoms.					
37203871	8	67	theme	increased	1475:1483	arg1	peak					1539:1542	postprandial H2 peak	1523:1542	postprandial H2 peak	1523:1542	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	8	67	theme	increased	1475:1483	arg1	fermentation					1509:1520	increased microbiota carbohydrate fermentation	1475:1520	increased microbiota carbohydrate fermentation (postprandial H2 peak)	1475:1543	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	3	68	dep	METHODS	374:380	arg1	assessed					408:415	assessed	408:415	assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis	408:723	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	1	69	theme	beneficial	165:174	arg1	effects					176:182	possible beneficial effects	156:182	possible beneficial effects	156:182	BACKGROUND Ramadan is a model of intermittent fasting linked with possible beneficial effects.					
37203871	8	70	theme	carbohydrate	1496:1507	arg1	peak					1539:1542	postprandial H2 peak	1523:1542	postprandial H2 peak	1523:1542	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	8	70	theme	carbohydrate	1496:1507	arg1	fermentation					1509:1520	increased microbiota carbohydrate fermentation	1475:1520	increased microbiota carbohydrate fermentation (postprandial H2 peak)	1475:1543	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	3	71	theme	transit	572:578	arg1	emptying					533:540	gastric/gallbladder emptying	513:540	gastric/gallbladder emptying by ultrasonography	513:559	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	71	theme	transit	572:578	arg1	time					580:583	orocaecal transit time	562:583	orocaecal transit time by lactulose breath test	562:608	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	8	72	theme	H2	1536:1537	arg1	peak					1539:1542	postprandial H2 peak	1523:1542	postprandial H2 peak	1523:1542	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	8	72	theme	H2	1536:1537	arg1	fermentation					1509:1520	increased microbiota carbohydrate fermentation	1475:1520	increased microbiota carbohydrate fermentation (postprandial H2 peak)	1475:1543	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	11	73	theme	diseased	1917:1924	arg1	people					1926:1931	diseased people	1917:1931	diseased people	1917:1931	Further comprehensive studies should assess the potential beneficial effects of RIF in diseased people.					
37203871	0	74	theme	intermittent	8:19	arg1	fasting					21:27	Ramadan intermittent fasting	0:27	Ramadan intermittent fasting	0:27	Ramadan intermittent fasting reduces visceral fat and improves gastrointestinal motility.					
37203871	7	75	theme	gallbladder	1298:1308	arg1	volume					1310:1315	Fasting gallbladder volume	1290:1315	Fasting gallbladder volume	1290:1315	Fasting gallbladder volume was about 6% smaller after, than before Ramadan, with a stronger and faster postprandial gallbladder contraction.					
37203871	4	76	theme	caloric	739:745	arg1	intake					747:752	Mean caloric intake	734:752	Mean caloric intake	734:752	RESULTS Mean caloric intake decreased from a median of 2069 kcal (range 1677-2641) before Ramadan to 1798 kcal (1289-3126) during Ramadan and increased again to 2000 kcal (1309-3485) after Ramadan.					
37203871	11	77	theme	RIF	1910:1912	arg1	effects					1899:1905	the potential beneficial effects	1874:1905	the potential beneficial effects of RIF in diseased people	1874:1931	Further comprehensive studies should assess the potential beneficial effects of RIF in diseased people.					
37203871	5	78	theme	subcutaneous	1138:1149	arg1	thickness					1168:1176	subcutaneous and visceral fat thickness	1138:1176	subcutaneous and visceral fat thickness	1138:1176	Although physical activity remained stable before, during, and after RIF, body weight, body mass index and waist circumference decreased in all subjects and in both genders, together with a significant decrease in subcutaneous and visceral fat thickness and insulin resistance.					
37203871	3	79	theme	visceral	653:660	arg1	ultrasonography					677:691	ultrasonography	677:691	ultrasonography	677:691	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	79	theme	visceral	653:660	arg1	thickness					666:674	subcutaneous and visceral fat thickness	636:674	subcutaneous and visceral fat thickness (ultrasonography)	636:692	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	80	theme	physical	454:461	arg1	activity					463:470	physical activity	454:470	physical activity	454:470	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	5	81	theme	visceral	1155:1162	arg1	thickness					1168:1176	subcutaneous and visceral fat thickness	1138:1176	subcutaneous and visceral fat thickness	1138:1176	Although physical activity remained stable before, during, and after RIF, body weight, body mass index and waist circumference decreased in all subjects and in both genders, together with a significant decrease in subcutaneous and visceral fat thickness and insulin resistance.					
37203871	8	82	theme	orocaecal	1557:1565	arg1	time					1575:1578	faster orocaecal transit time	1550:1578	faster orocaecal transit time	1550:1578	After RIF, lactulose breath test documented increased microbiota carbohydrate fermentation (postprandial H2 peak), and faster orocaecal transit time.					
37203871	3	83	theme	subcutaneous	636:647	arg1	ultrasonography					677:691	ultrasonography	677:691	ultrasonography	677:691	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	83	theme	subcutaneous	636:647	arg1	thickness					666:674	subcutaneous and visceral fat thickness	636:674	subcutaneous and visceral fat thickness (ultrasonography)	636:692	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	10	84	theme	healthy	1692:1698	arg1	subjects					1700:1707	healthy subjects	1692:1707	healthy subjects	1692:1707	CONCLUSIONS RIF generates, in healthy subjects, multiple systemic beneficial effects in terms of fat burden, metabolic profile, gastrointestinal motility and symptoms.					
37203871	2	85	theme	combined	237:244	arg1	effects					246:252	the combined effects	233:252	the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility	233:371	Scarce information, however, is available about the combined effects of Ramadan intermittent fasting (RIF) on anthropometric and metabolic indices, gastrointestinal symptoms, and motility.					
37203871	3	86	dep	symptoms	490:497	arg1	emptying					533:540	gastric/gallbladder emptying	513:540	gastric/gallbladder emptying by ultrasonography	513:559	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	86	dep	symptoms	490:497	arg1	time					580:583	orocaecal transit time	562:583	orocaecal transit time by lactulose breath test	562:608	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	87	from	impact	421:426	arg1	activity					463:470	physical activity	454:470	physical activity	454:470	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	87	from	impact	421:426	arg1	glucose					695:701	glucose	695:701	glucose	695:701	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	87	from	impact	421:426	arg1	symptoms					490:497	gastrointestinal symptoms	473:497	gastrointestinal symptoms	473:497	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	87	from	impact	421:426	arg1	indices					627:633	anthropometric indices	612:633	anthropometric indices	612:633	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	87	from	impact	421:426	arg1	thickness					666:674	subcutaneous and visceral fat thickness	636:674	subcutaneous and visceral fat thickness (ultrasonography)	636:692	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	87	from	impact	421:426	arg1	intake					446:451	caloric intake	438:451	caloric intake	438:451	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	87	from	impact	421:426	arg1	ultrasonography					677:691	ultrasonography	677:691	ultrasonography	677:691	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	87	from	impact	421:426	arg1	homeostasis					713:723	lipid homeostasis	707:723	lipid homeostasis	707:723	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	88	theme	gastric/gallbladder	513:531	arg1	emptying					533:540	gastric/gallbladder emptying	513:540	gastric/gallbladder emptying by ultrasonography	513:559	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	3	88	theme	gastric/gallbladder	513:531	arg1	time					580:583	orocaecal transit time	562:583	orocaecal transit time by lactulose breath test	562:608	METHODS In 21 healthy Muslims, we assessed the impact of RIF on caloric intake, physical activity, gastrointestinal symptoms and motility (gastric/gallbladder emptying by ultrasonography, orocaecal transit time by lactulose breath test), anthropometric indices, subcutaneous and visceral fat thickness (ultrasonography), glucose and lipid homeostasis.					
37203871	10	89	theme	systemic	1719:1726	arg1	effects					1739:1745	multiple systemic beneficial effects	1710:1745	multiple systemic beneficial effects	1710:1745	CONCLUSIONS RIF generates, in healthy subjects, multiple systemic beneficial effects in terms of fat burden, metabolic profile, gastrointestinal motility and symptoms.					
35817849	4	0	theme	MHL	1231:1233	arg1	P < 0.05					1251:1258	P < 0.05	1251:1258	P < 0.05	1251:1258	RESULTS People with MHO performed ~45-min more light-intensity physical activity/day than the MHL and MUO groups (P < 0.05).					
35817849	4	0	theme	MHL	1231:1233	arg1	groups					1243:1248	the MHL and MUO groups	1227:1248	the MHL and MUO groups (P < 0.05)	1227:1259	RESULTS People with MHO performed ~45-min more light-intensity physical activity/day than the MHL and MUO groups (P < 0.05).					
35817849	5	1	theme	MUO	1341:1343	arg1	group					1345:1349	the MUO group	1337:1349	the MUO group (P < 0.05)	1337:1360	QOL, particularly the physical function domain, was higher in the MHO than the MUO group (P < 0.05).					
35817849	5	1	theme	MUO	1341:1343	arg1	P < 0.05					1352:1359	P < 0.05	1352:1359	P < 0.05	1352:1359	QOL, particularly the physical function domain, was higher in the MHO than the MUO group (P < 0.05).					
35817849	6	2	theme	dairy	1404:1408	arg1	intake					1386:1391	self-reported intake	1372:1391	self-reported intake of starch, dairy, and cured meats	1372:1425	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	3	3	dep	obesity	957:963	arg1	2					954:954	2	954:954	2	954:954	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	1	4	theme	metabolic	246:254	arg1	abnormalities					256:268	metabolic abnormalities	246:268	metabolic abnormalities	246:268	BACKGROUND AND OBJECTIVES Although obesity is typically associated with metabolic co-morbidities, some people with obesity do not develop metabolic abnormalities.					
35817849	3	5	theme	sleep	683:687	arg1	quality					689:695	sleep quality	683:695	sleep quality	683:695	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	2	6	dep	healthy	459:465	arg1	MHO					468:470	MHO	468:470	MHO	468:470	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	2	6	dep	healthy	459:465	arg1	people					421:426	some people	416:426	some people with obesity	416:439	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	3	7	theme	triglyceride	842:853	arg1	concentration					860:872	plasma triglyceride (TG) concentration	835:872	plasma triglyceride (TG) concentration	835:872	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	8	theme	normal	809:814	arg1	tolerance					824:832	normal glucose tolerance	809:832	normal glucose tolerance	809:832	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	9	theme	glucose	1081:1087	arg1	metabolism					1089:1098	abnormal glucose metabolism	1072:1098	abnormal glucose metabolism	1072:1098	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	5	10	theme	physical	1284:1291	arg1	QOL					1262:1264	QOL	1262:1264	QOL	1262:1264	QOL, particularly the physical function domain, was higher in the MHO than the MUO group (P < 0.05).					
35817849	5	10	theme	physical	1284:1291	arg1	domain					1302:1307	the physical function domain	1280:1307	particularly the physical function domain	1267:1307	QOL, particularly the physical function domain, was higher in the MHO than the MUO group (P < 0.05).					
35817849	3	11	theme	glucose	976:982	arg1	tolerance					984:992	normal glucose tolerance	969:992	normal glucose tolerance	969:992	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	2	12	theme	lifestyle	303:311	arg1	factors					313:319	modifiable lifestyle factors	292:319	modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics)	292:393	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	8	13	with	people	1814:1819	arg1	MUO					1826:1828	MUO	1826:1828	MUO	1826:1828	CONCLUSIONS These data suggest physical activity, but not sleep or dietary intake, contribute to better metabolic health in people with MHO than those with MUO, and that QOL is lower in people with MUO than those with MHO.					
35817849	0	14	theme	healthy	77:83	arg1	obesity					99:105	metabolically healthy and unhealthy obesity	63:105	metabolically healthy and unhealthy obesity	63:105	Do lifestyle factors and quality of life differ in people with metabolically healthy and unhealthy obesity?					
35817849	4	15	theme	physical	1200:1207	arg1	activity/day					1209:1220	light-intensity physical activity/day	1184:1220	light-intensity physical activity/day	1184:1220	RESULTS People with MHO performed ~45-min more light-intensity physical activity/day than the MHL and MUO groups (P < 0.05).					
35817849	0	16	with	people	51:56	arg1	obesity					99:105	metabolically healthy and unhealthy obesity	63:105	metabolically healthy and unhealthy obesity	63:105	Do lifestyle factors and quality of life differ in people with metabolically healthy and unhealthy obesity?					
35817849	2	17	theme	modifiable	292:301	arg1	factors					313:319	modifiable lifestyle factors	292:319	modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics)	292:393	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	3	18	theme	intrahepatic	878:889	arg1	[MHL					937:940	metabolically healthy lean [MHL	910:940	metabolically healthy lean [MHL	910:940	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	18	theme	intrahepatic	878:889	arg1	content					901:907	intrahepatic TG (IHTG) content	878:907	intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]	878:941	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	8	19	from	health	1742:1747	arg1	people					1752:1757	people	1752:1757	people with MHO	1752:1766	CONCLUSIONS These data suggest physical activity, but not sleep or dietary intake, contribute to better metabolic health in people with MHO than those with MUO, and that QOL is lower in people with MUO than those with MHO.					
35817849	4	20	theme	light-intensity	1184:1198	arg1	activity/day					1209:1220	light-intensity physical activity/day	1184:1220	light-intensity physical activity/day	1184:1220	RESULTS People with MHO performed ~45-min more light-intensity physical activity/day than the MHL and MUO groups (P < 0.05).					
35817849	8	21	theme	dietary	1695:1701	arg1	intake					1703:1708	dietary intake	1695:1708	dietary intake	1695:1708	CONCLUSIONS These data suggest physical activity, but not sleep or dietary intake, contribute to better metabolic health in people with MHO than those with MUO, and that QOL is lower in people with MUO than those with MHO.					
35817849	6	22	theme	MHO	1459:1461	arg1	group					1463:1467	the MHO group	1455:1467	the MHO group (P < 0.02)	1455:1478	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	6	22	theme	MHO	1459:1461	arg1	P < 0.02					1470:1477	P < 0.02	1470:1477	P < 0.02	1470:1477	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	3	23	theme	TG	891:892	arg1	[MHL					937:940	metabolically healthy lean [MHL	910:940	metabolically healthy lean [MHL	910:940	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	23	theme	TG	891:892	arg1	content					901:907	intrahepatic TG (IHTG) content	878:907	intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]	878:941	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	0	24	theme	unhealthy	89:97	arg1	obesity					99:105	metabolically healthy and unhealthy obesity	63:105	metabolically healthy and unhealthy obesity	63:105	Do lifestyle factors and quality of life differ in people with metabolically healthy and unhealthy obesity?					
35817849	2	25	with	people	421:426	arg1	obesity					433:439	obesity	433:439	obesity	433:439	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	3	26	theme	IHTG	1023:1026	arg1	content					1028:1034	IHTG content	1023:1034	IHTG content	1023:1034	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	27	from	questionnaires	740:753	arg1	people					758:763	people	758:763	people stratified into three groups	758:792	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	6	28	theme	metabolic	1538:1546	arg1	effects					1548:1554	metabolic effects	1538:1554	metabolic effects	1538:1554	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	0	29	theme	lifestyle	3:11	arg1	factors					13:19	lifestyle factors	3:19	lifestyle factors	3:19	Do lifestyle factors and quality of life differ in people with metabolically healthy and unhealthy obesity?					
35817849	3	30	theme	TG	1002:1003	arg1	MHO					1037:1039	MHO; n = 36	1037:1047	MHO; n = 36	1037:1047	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	30	theme	TG	1002:1003	arg1	concentration					1005:1017	plasma TG concentration	995:1017	plasma TG concentration	995:1017	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	31	theme	normal	969:974	arg1	tolerance					984:992	normal glucose tolerance	969:992	normal glucose tolerance	969:992	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	2	32	dep	activity	337:344	arg1	i.e.					322:325	i.e.	322:325	i.e.	322:325	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	1	33	theme	metabolic	180:188	arg1	co-morbidities					190:203	metabolic co-morbidities	180:203	metabolic co-morbidities	180:203	BACKGROUND AND OBJECTIVES Although obesity is typically associated with metabolic co-morbidities, some people with obesity do not develop metabolic abnormalities.					
35817849	4	34	theme	MUO	1239:1241	arg1	P < 0.05					1251:1258	P < 0.05	1251:1258	P < 0.05	1251:1258	RESULTS People with MHO performed ~45-min more light-intensity physical activity/day than the MHL and MUO groups (P < 0.05).					
35817849	4	34	theme	MUO	1239:1241	arg1	groups					1243:1248	the MHL and MUO groups	1227:1248	the MHL and MUO groups (P < 0.05)	1227:1259	RESULTS People with MHO performed ~45-min more light-intensity physical activity/day than the MHL and MUO groups (P < 0.05).					
35817849	5	35	theme	function	1293:1300	arg1	QOL					1262:1264	QOL	1262:1264	QOL	1262:1264	QOL, particularly the physical function domain, was higher in the MHO than the MUO group (P < 0.05).					
35817849	5	35	theme	function	1293:1300	arg1	domain					1302:1307	the physical function domain	1280:1307	particularly the physical function domain	1267:1307	QOL, particularly the physical function domain, was higher in the MHO than the MUO group (P < 0.05).					
35817849	6	36	theme	self-reported	1372:1384	arg1	intake					1386:1391	self-reported intake	1372:1391	self-reported intake of starch, dairy, and cured meats	1372:1425	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	3	37	theme	plasma	995:1000	arg1	MHO					1037:1039	MHO; n = 36	1037:1047	MHO; n = 36	1037:1047	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	37	theme	plasma	995:1000	arg1	concentration					1005:1017	plasma TG concentration	995:1017	plasma TG concentration	995:1017	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	38	with	obesity	957:963	arg1	steatosis					1112:1120	hepatic steatosis	1104:1120	hepatic steatosis (MUO; n = 43)	1104:1134	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	38	with	obesity	957:963	arg1	MUO					1123:1125	MUO	1123:1125	MUO	1123:1125	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	38	with	obesity	957:963	arg1	metabolism					1089:1098	abnormal glucose metabolism	1072:1098	abnormal glucose metabolism	1072:1098	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	1	39	with	people	211:216	arg1	obesity					223:229	obesity	223:229	obesity	223:229	BACKGROUND AND OBJECTIVES Although obesity is typically associated with metabolic co-morbidities, some people with obesity do not develop metabolic abnormalities.					
35817849	8	40	theme	physical	1659:1666	arg1	activity					1668:1675	physical activity	1659:1675	physical activity	1659:1675	CONCLUSIONS These data suggest physical activity, but not sleep or dietary intake, contribute to better metabolic health in people with MHO than those with MUO, and that QOL is lower in people with MUO than those with MHO.					
35817849	8	41	dep	CONCLUSIONS	1628:1638	arg1	suggest					1651:1657	suggest	1651:1657	suggest physical activity, but not sleep or dietary intake, contribute to better metabolic health in people with MHO than those with MUO, and that QOL is lower in people with MUO than those with MHO	1651:1848	CONCLUSIONS These data suggest physical activity, but not sleep or dietary intake, contribute to better metabolic health in people with MHO than those with MUO, and that QOL is lower in people with MUO than those with MHO.					
35817849	3	42	with	content	1028:1034	arg1	steatosis					1112:1120	hepatic steatosis	1104:1120	hepatic steatosis (MUO; n = 43)	1104:1134	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	42	with	content	1028:1034	arg1	MUO					1123:1125	MUO	1123:1125	MUO	1123:1125	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	42	with	content	1028:1034	arg1	metabolism					1089:1098	abnormal glucose metabolism	1072:1098	abnormal glucose metabolism	1072:1098	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	43	theme	hepatic	1104:1110	arg1	steatosis					1112:1120	hepatic steatosis	1104:1120	hepatic steatosis (MUO; n = 43)	1104:1134	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	43	theme	hepatic	1104:1110	arg1	MUO					1123:1125	MUO	1123:1125	MUO	1123:1125	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	44	theme	tri-axial	638:646	arg1	accelerometers					648:661	tri-axial accelerometers	638:661	tri-axial accelerometers	638:661	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	4	45	with	People	1145:1150	arg1	MHO					1157:1159	MHO	1157:1159	MHO	1157:1159	RESULTS People with MHO performed ~45-min more light-intensity physical activity/day than the MHL and MUO groups (P < 0.05).					
35817849	3	46	theme	validated	730:738	arg1	questionnaires					740:753	validated questionnaires	730:753	validated questionnaires in people stratified into three groups	730:792	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	8	47	theme	metabolic	1732:1740	arg1	health					1742:1747	better metabolic health	1725:1747	better metabolic health in people with MHO than those with MUO	1725:1786	CONCLUSIONS These data suggest physical activity, but not sleep or dietary intake, contribute to better metabolic health in people with MHO than those with MUO, and that QOL is lower in people with MUO than those with MHO.					
35817849	3	48	theme	IHTG	895:898	arg1	[MHL					937:940	metabolically healthy lean [MHL	910:940	metabolically healthy lean [MHL	910:940	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	48	theme	IHTG	895:898	arg1	content					901:907	intrahepatic TG (IHTG) content	878:907	intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]	878:941	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	6	49	contain	have	1533:1536	arg1	small					1511:1515	small	1511:1515	small	1511:1515	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	6	49	contain	have	1533:1536	arg2	effects					1548:1554	metabolic effects	1538:1554	metabolic effects	1538:1554	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	6	49	contain	have	1533:1536	arg1	differences					1494:1504	the absolute differences	1481:1504	the absolute differences	1481:1504	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	3	50	dep	obesity	1059:1065	arg1	3					1056:1056	3	1056:1056	3	1056:1056	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	4	51	theme	more	1179:1182	arg1	~45-min					1171:1177	~45-min	1171:1177	~45-min more light-intensity physical activity/day than the MHL and MUO groups (P < 0.05)	1171:1259	RESULTS People with MHO performed ~45-min more light-intensity physical activity/day than the MHL and MUO groups (P < 0.05).					
35817849	3	52	theme	healthy	924:930	arg1	[MHL					937:940	metabolically healthy lean [MHL	910:940	metabolically healthy lean [MHL	910:940	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	52	theme	healthy	924:930	arg1	content					901:907	intrahepatic TG (IHTG) content	878:907	intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]	878:941	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	53	theme	abnormal	1072:1079	arg1	metabolism					1089:1098	abnormal glucose metabolism	1072:1098	abnormal glucose metabolism	1072:1098	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	4	54	dep	more	1179:1182	arg1	activity/day					1209:1220	light-intensity physical activity/day	1184:1220	light-intensity physical activity/day	1184:1220	RESULTS People with MHO performed ~45-min more light-intensity physical activity/day than the MHL and MUO groups (P < 0.05).					
35817849	3	55	theme	SUBJECTS/METHODS	554:569	arg1	activity					580:587	SUBJECTS/METHODS Physical activity	554:587	SUBJECTS/METHODS Physical activity	554:587	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	56	theme	lean	932:935	arg1	[MHL					937:940	metabolically healthy lean [MHL	910:940	metabolically healthy lean [MHL	910:940	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	56	theme	lean	932:935	arg1	content					901:907	intrahepatic TG (IHTG) content	878:907	intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]	878:941	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	2	57	theme	dietary	347:353	arg1	composition					355:365	dietary composition	347:365	dietary composition	347:365	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	0	58	theme	life	36:39	arg1	factors					13:19	lifestyle factors	3:19	lifestyle factors	3:19	Do lifestyle factors and quality of life differ in people with metabolically healthy and unhealthy obesity?					
35817849	0	58	theme	life	36:39	arg1	quality					25:31	quality	25:31	quality of life	25:39	Do lifestyle factors and quality of life differ in people with metabolically healthy and unhealthy obesity?					
35817849	2	59	theme	life	542:545	arg1	quality					531:537	quality	531:537	quality of life (QOL)	531:551	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	6	60	theme	absolute	1485:1492	arg1	small					1511:1515	small	1511:1515	small	1511:1515	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	6	60	theme	absolute	1485:1492	arg1	differences					1494:1504	the absolute differences	1481:1504	the absolute differences	1481:1504	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	3	61	theme	Physical	571:578	arg1	activity					580:587	SUBJECTS/METHODS Physical activity	554:587	SUBJECTS/METHODS Physical activity	554:587	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	62	with	concentration	1005:1017	arg1	steatosis					1112:1120	hepatic steatosis	1104:1120	hepatic steatosis (MUO; n = 43)	1104:1134	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	62	with	concentration	1005:1017	arg1	MUO					1123:1125	MUO	1123:1125	MUO	1123:1125	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	62	with	concentration	1005:1017	arg1	metabolism					1089:1098	abnormal glucose metabolism	1072:1098	abnormal glucose metabolism	1072:1098	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	8	63	with	people	1752:1757	arg1	MHO					1764:1766	MHO	1764:1766	MHO	1764:1766	CONCLUSIONS These data suggest physical activity, but not sleep or dietary intake, contribute to better metabolic health in people with MHO than those with MUO, and that QOL is lower in people with MUO than those with MHO.					
35817849	8	64	theme	better	1725:1730	arg1	health					1742:1747	better metabolic health	1725:1747	better metabolic health in people with MHO than those with MUO	1725:1786	CONCLUSIONS These data suggest physical activity, but not sleep or dietary intake, contribute to better metabolic health in people with MHO than those with MUO, and that QOL is lower in people with MUO than those with MHO.					
35817849	6	65	theme	meats	1421:1425	arg1	intake					1386:1391	self-reported intake	1372:1391	self-reported intake of starch, dairy, and cured meats	1372:1425	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	3	66	theme	plasma	835:840	arg1	concentration					860:872	plasma triglyceride (TG) concentration	835:872	plasma triglyceride (TG) concentration	835:872	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	6	67	theme	starch	1396:1401	arg1	intake					1386:1391	self-reported intake	1372:1391	self-reported intake of starch, dairy, and cured meats	1372:1425	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	6	68	theme	cured	1415:1419	arg1	meats					1421:1425	cured meats	1415:1425	cured meats	1415:1425	Although self-reported intake of starch, dairy, and cured meats were higher in the MUO than the MHO group (P < 0.02), the absolute differences were small and unlikely to have metabolic effects.					
35817849	2	69	theme	physical	328:335	arg1	activity					337:344	physical activity	328:344	physical activity	328:344	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	3	70	theme	glucose	816:822	arg1	tolerance					824:832	normal glucose tolerance	809:832	normal glucose tolerance	809:832	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	8	71	dep	suggest	1651:1657	arg1	lower					1805:1809	lower	1805:1809	lower	1805:1809	CONCLUSIONS These data suggest physical activity, but not sleep or dietary intake, contribute to better metabolic health in people with MHO than those with MUO, and that QOL is lower in people with MUO than those with MHO.					
35817849	8	71	dep	suggest	1651:1657	arg1	contribute					1711:1720	contribute	1711:1720	contribute to better metabolic health in people with MHO than those with MUO	1711:1786	CONCLUSIONS These data suggest physical activity, but not sleep or dietary intake, contribute to better metabolic health in people with MHO than those with MUO, and that QOL is lower in people with MUO than those with MHO.					
35817849	2	72	theme	unhealthy	499:507	arg1	MUO					518:520	MUO	518:520	MUO	518:520	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	2	72	theme	unhealthy	499:507	arg1	obesity					509:515	metabolically unhealthy obesity	485:515	metabolically unhealthy obesity (MUO)	485:521	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	3	73	theme	dietary	667:673	arg1	intake					675:680	dietary intake	667:680	dietary intake	667:680	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	74	theme	sleep	593:597	arg1	characteristics					599:613	sleep characteristics	593:613	sleep characteristics	593:613	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	2	75	theme	sleep	372:376	arg1	characteristics					378:392	sleep characteristics	372:392	sleep characteristics	372:392	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	4	76	dep	RESULTS	1137:1143	arg1	performed					1161:1169	performed	1161:1169	performed ~45-min more light-intensity physical activity/day than the MHL and MUO groups (P < 0.05)	1161:1259	RESULTS People with MHO performed ~45-min more light-intensity physical activity/day than the MHL and MUO groups (P < 0.05).					
35817849	2	77	theme	metabolically	485:497	arg1	MUO					518:520	MUO	518:520	MUO	518:520	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	2	77	theme	metabolically	485:497	arg1	obesity					509:515	metabolically unhealthy obesity	485:515	metabolically unhealthy obesity (MUO)	485:521	We evaluated whether modifiable lifestyle factors (i.e., physical activity, dietary composition, and sleep characteristics) can help explain why some people with obesity are metabolically healthy (MHO) and whether metabolically unhealthy obesity (MUO) affects quality of life (QOL).					
35817849	3	78	dep	using	724:728	arg1	1					796:796	1	796:796	1	796:796	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	79	with	obesity	1059:1065	arg1	steatosis					1112:1120	hepatic steatosis	1104:1120	hepatic steatosis (MUO; n = 43)	1104:1134	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	79	with	obesity	1059:1065	arg1	MUO					1123:1125	MUO	1123:1125	MUO	1123:1125	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	79	with	obesity	1059:1065	arg1	metabolism					1089:1098	abnormal glucose metabolism	1072:1098	abnormal glucose metabolism	1072:1098	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	80	with	tolerance	984:992	arg1	steatosis					1112:1120	hepatic steatosis	1104:1120	hepatic steatosis (MUO; n = 43)	1104:1134	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	80	with	tolerance	984:992	arg1	MUO					1123:1125	MUO	1123:1125	MUO	1123:1125	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	80	with	tolerance	984:992	arg1	metabolism					1089:1098	abnormal glucose metabolism	1072:1098	abnormal glucose metabolism	1072:1098	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
35817849	3	81	theme	TG	856:857	arg1	concentration					860:872	plasma triglyceride (TG) concentration	835:872	plasma triglyceride (TG) concentration	835:872	SUBJECTS/METHODS Physical activity and sleep characteristics were assessed by using tri-axial accelerometers and dietary intake, sleep quality, and QOL were evaluated by using validated questionnaires in people stratified into three groups: (1) lean with normal glucose tolerance, plasma triglyceride (TG) concentration and intrahepatic TG (IHTG) content (metabolically healthy lean [MHL]; n = 20); (2) obesity and normal glucose tolerance, plasma TG concentration and IHTG content (MHO; n = 36); and (3) obesity with abnormal glucose metabolism and hepatic steatosis (MUO; n = 43).					
36736519	0	0	theme	MC3T3-E1	98:105	arg1	cells					107:111	MC3T3-E1 cells	98:111	MC3T3-E1 cells	98:111	Silk fibroin-chitosan aerogel reinforced by nanofibers for enhanced osteogenic differentiation in MC3T3-E1 cells.					
36736519	6	1	theme	Counting	867:874	arg1	CCK-8					883:887	CCK-8	883:887	CCK-8	883:887	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	6	1	theme	Counting	867:874	arg1	Kit-8					876:880	Cell Counting Kit-8	862:880	Cell Counting Kit-8 (CCK-8) assays	862:895	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	6	2	theme	aerogel	826:832	arg1	scaffolds					834:842	the aerogel scaffolds	822:842	the aerogel scaffolds	822:842	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	9	3	theme	well-regulated	1459:1472	arg1	pores					1474:1478	well-regulated pores	1459:1478	well-regulated pores	1459:1478	This biocompatible nanofiber-reinforced aerogel scaffold facilitates osteogenic differentiation by rougher surface, enhanced mechanical strength and well-regulated pores.					
36736519	6	4	theme	microscopy	944:953	arg1	images					961:966	confocal microscopy and scanning electron microscopy (SEM) images	902:966	confocal microscopy and scanning electron microscopy (SEM) images	902:966	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	5	5	theme	regulated	722:730	arg1	size					737:740	the regulated pore size	718:740	the regulated pore size	718:740	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	6	6	theme	Cell	862:865	arg1	CCK-8					883:887	CCK-8	883:887	CCK-8	883:887	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	6	6	theme	Cell	862:865	arg1	Kit-8					876:880	Cell Counting Kit-8	862:880	Cell Counting Kit-8 (CCK-8) assays	862:895	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	7	7	theme	aerogel	1058:1064	arg1	scaffolds					1066:1074	the aerogel scaffolds	1054:1074	the aerogel scaffolds	1054:1074	The cell differentiation on the aerogel scaffolds was evaluated with enzyme-linked immunosorbent assay (ELISA).					
36736519	2	8	theme	SF-CS/NF1	325:333	arg1	%					334:334	SF-CS/NF1%	325:334	SF-CS/NF1%	325:334	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	6	9	theme	electron	935:942	arg1	SEM					956:958	SEM	956:958	SEM	956:958	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	6	9	theme	electron	935:942	arg1	microscopy					944:953	scanning electron microscopy	926:953	scanning electron microscopy (SEM)	926:959	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	5	10	theme	high	687:690	arg1	porosity					692:699	the high porosity	683:699	the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %	683:792	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	8	11	theme	%	1298:1298	arg1	aerogels					1300:1307	SF-CS/NF3% aerogels	1289:1307	SF-CS/NF3% aerogels	1289:1307	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	8	12	theme	alkaline	1235:1242	arg1	phosphatase					1244:1254	alkaline phosphatase	1235:1254	alkaline phosphatase (ALP) expression	1235:1271	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	8	12	theme	alkaline	1235:1242	arg1	ALP					1257:1259	ALP	1257:1259	ALP	1257:1259	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	2	13	dep	nanofibers	313:322	arg1	%					334:334	SF-CS/NF1%	325:334	SF-CS/NF1%	325:334	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	2	13	dep	nanofibers	313:322	arg1	%					346:346	SF-CS/NF2%	337:346	SF-CS/NF2%	337:346	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	2	13	dep	nanofibers	313:322	arg1	%					361:361	SF-CS/NF3%	352:361	SF-CS/NF3%	352:361	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	6	14	theme	scanning	926:933	arg1	SEM					956:958	SEM	956:958	SEM	956:958	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	6	14	theme	scanning	926:933	arg1	microscopy					944:953	scanning electron microscopy	926:953	scanning electron microscopy (SEM)	926:959	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	10	15	theme	bone	1535:1538	arg1	field					1553:1557	bone regeneration field	1535:1557	bone regeneration field	1535:1557	Thus, as-prepared scaffolds may be further applied in bone regeneration field.					
36736519	1	16	dep	biocompatible	146:158	arg1	robust					174:179	robust	174:179	robust	174:179	Proper bone scaffolds should be biocompatible, mechanically robust and porous for cell migration.					
36736519	2	17	theme	aerogel	256:262	arg1	scaffolds					264:272	pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds	218:272	pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%)	218:362	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	8	18	theme	type	1177:1180	arg1	level					1159:1163	Significantly higher level	1138:1163	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression	1138:1271	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	10	19	theme	regeneration	1540:1551	arg1	field					1553:1557	bone regeneration field	1535:1557	bone regeneration field	1535:1557	Thus, as-prepared scaffolds may be further applied in bone regeneration field.					
36736519	5	20	theme	high-water	750:759	arg1	ratio					768:772	the high-water uptake ratio	746:772	the high-water uptake ratio	746:772	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	4	21	with	endowed	530:536	arg1	resistance					558:567	a resistance	556:567	a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels	556:630	Incorporating 3 % nanofibers endowed the aerogels with a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels.					
36736519	6	22	theme	cell	993:996	arg1	migration					998:1006	the cell migration	989:1006	the cell migration	989:1006	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	4	23	theme	aerogels	623:630	arg1	stress					598:603	the compressive stress	582:603	3.5 times the compressive stress of the pure SF-CS aerogels	572:630	Incorporating 3 % nanofibers endowed the aerogels with a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels.					
36736519	9	24	theme	biocompatible	1315:1327	arg1	scaffold					1358:1365	This biocompatible nanofiber-reinforced aerogel scaffold	1310:1365	This biocompatible nanofiber-reinforced aerogel scaffold	1310:1365	This biocompatible nanofiber-reinforced aerogel scaffold facilitates osteogenic differentiation by rougher surface, enhanced mechanical strength and well-regulated pores.					
36736519	6	25	theme	scaffolds	834:842	arg1	viability					809:817	Enhanced cell viability	795:817	Enhanced cell viability of the aerogel scaffolds	795:842	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	7	26	theme	cell	1030:1033	arg1	differentiation					1035:1049	The cell differentiation	1026:1049	The cell differentiation on the aerogel scaffolds	1026:1074	The cell differentiation on the aerogel scaffolds was evaluated with enzyme-linked immunosorbent assay (ELISA).					
36736519	5	27	theme	uptake	761:766	arg1	ratio					768:772	the high-water uptake ratio	746:772	the high-water uptake ratio	746:772	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	3	28	theme	Surface	400:406	arg1	morphology					408:417	Surface morphology	400:417	Surface morphology	400:417	Surface morphology and composition were analyzed to ensure successful integration of each component.					
36736519	0	29	theme	fibroin-chitosan	5:20	arg1	aerogel					22:28	Silk fibroin-chitosan aerogel	0:28	Silk fibroin-chitosan aerogel	0:28	Silk fibroin-chitosan aerogel reinforced by nanofibers for enhanced osteogenic differentiation in MC3T3-E1 cells.					
36736519	4	30	dep	nanofibers	519:528	arg1	endowed					530:536	endowed	530:536	endowed	530:536	Incorporating 3 % nanofibers endowed the aerogels with a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels.					
36736519	10	31	theme	as-prepared	1487:1497	arg1	scaffolds					1499:1507	as-prepared scaffolds	1487:1507	as-prepared scaffolds	1487:1507	Thus, as-prepared scaffolds may be further applied in bone regeneration field.					
36736519	1	32	theme	cell	196:199	arg1	migration					201:209	cell migration	196:209	cell migration	196:209	Proper bone scaffolds should be biocompatible, mechanically robust and porous for cell migration.					
36736519	0	33	theme	Silk	0:3	arg1	aerogel					22:28	Silk fibroin-chitosan aerogel	0:28	Silk fibroin-chitosan aerogel	0:28	Silk fibroin-chitosan aerogel reinforced by nanofibers for enhanced osteogenic differentiation in MC3T3-E1 cells.					
36736519	6	34	theme	microscopy	911:920	arg1	images					961:966	confocal microscopy and scanning electron microscopy (SEM) images	902:966	confocal microscopy and scanning electron microscopy (SEM) images	902:966	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	2	35	theme	different	290:298	arg1	nanofibers					313:322	SF nanofibers	310:322	SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%)	310:362	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	2	35	theme	different	290:298	arg1	amount					300:305	different amount	290:305	different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%)	290:362	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	5	36	theme	ratio	768:772	arg1	porosity					692:699	the high porosity	683:699	the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %	683:792	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	6	37	theme	confocal	902:909	arg1	microscopy					911:920	confocal microscopy	902:920	confocal microscopy	902:920	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	8	38	theme	SF-CS/NF3	1289:1297	arg1	aerogels					1300:1307	SF-CS/NF3% aerogels	1289:1307	SF-CS/NF3% aerogels	1289:1307	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	1	39	theme	Proper	114:119	arg1	scaffolds					126:134	Proper bone scaffolds	114:134	Proper bone scaffolds	114:134	Proper bone scaffolds should be biocompatible, mechanically robust and porous for cell migration.					
36736519	9	40	theme	osteogenic	1379:1388	arg1	differentiation					1390:1404	osteogenic differentiation	1379:1404	osteogenic differentiation	1379:1404	This biocompatible nanofiber-reinforced aerogel scaffold facilitates osteogenic differentiation by rougher surface, enhanced mechanical strength and well-regulated pores.					
36736519	2	41	theme	bone	381:384	arg1	regeneration					386:397	bone regeneration	381:397	bone regeneration	381:397	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	6	42	theme	Enhanced	795:802	arg1	viability					809:817	Enhanced cell viability	795:817	Enhanced cell viability of the aerogel scaffolds	795:842	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	1	43	theme	bone	121:124	arg1	scaffolds					126:134	Proper bone scaffolds	114:134	Proper bone scaffolds	114:134	Proper bone scaffolds should be biocompatible, mechanically robust and porous for cell migration.					
36736519	5	44	theme	%	792:792	arg1	ratio					768:772	the high-water uptake ratio	746:772	the high-water uptake ratio	746:772	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	5	44	theme	%	792:792	arg1	size					737:740	the regulated pore size	718:740	the regulated pore size	718:740	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	5	44	theme	%	792:792	arg1	%					715:715	72.3 ± 1.3 %	704:715	72.3 ± 1.3 %	704:715	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	5	44	theme	%	792:792	arg1	%					792:792	1770.4 ± 156.8 %	777:792	1770.4 ± 156.8 %	777:792	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	5	45	theme	pore	732:735	arg1	size					737:740	the regulated pore size	718:740	the regulated pore size	718:740	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	3	46	theme	successful	459:468	arg1	integration					470:480	successful integration	459:480	successful integration of each component	459:498	Surface morphology and composition were analyzed to ensure successful integration of each component.					
36736519	4	47	theme	pure	612:615	arg1	aerogels					623:630	the pure SF-CS aerogels	608:630	the pure SF-CS aerogels	608:630	Incorporating 3 % nanofibers endowed the aerogels with a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels.					
36736519	5	48	theme	size	737:740	arg1	porosity					692:699	the high porosity	683:699	the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %	683:792	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	7	49	from	differentiation	1035:1049	arg1	scaffolds					1066:1074	the aerogel scaffolds	1054:1074	the aerogel scaffolds	1054:1074	The cell differentiation on the aerogel scaffolds was evaluated with enzyme-linked immunosorbent assay (ELISA).					
36736519	8	50	theme	higher	1152:1157	arg1	level					1159:1163	Significantly higher level	1138:1163	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression	1138:1271	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	0	51	from	differentiation	79:93	arg1	cells					107:111	MC3T3-E1 cells	98:111	MC3T3-E1 cells	98:111	Silk fibroin-chitosan aerogel reinforced by nanofibers for enhanced osteogenic differentiation in MC3T3-E1 cells.					
36736519	7	52	theme	enzyme-linked	1095:1107	arg1	ELISA					1130:1134	ELISA	1130:1134	ELISA	1130:1134	The cell differentiation on the aerogel scaffolds was evaluated with enzyme-linked immunosorbent assay (ELISA).					
36736519	7	52	theme	enzyme-linked	1095:1107	arg1	assay					1123:1127	enzyme-linked immunosorbent assay	1095:1127	enzyme-linked immunosorbent assay (ELISA)	1095:1135	The cell differentiation on the aerogel scaffolds was evaluated with enzyme-linked immunosorbent assay (ELISA).					
36736519	8	53	located	observed	1277:1284	arg2	level					1159:1163	Significantly higher level	1138:1163	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression	1138:1271	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	8	53	located	observed	1277:1284	arg1	aerogels					1300:1307	SF-CS/NF3% aerogels	1289:1307	SF-CS/NF3% aerogels	1289:1307	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	0	54	theme	enhanced	59:66	arg1	differentiation					79:93	enhanced osteogenic differentiation	59:93	enhanced osteogenic differentiation in MC3T3-E1 cells	59:111	Silk fibroin-chitosan aerogel reinforced by nanofibers for enhanced osteogenic differentiation in MC3T3-E1 cells.					
36736519	9	55	theme	aerogel	1350:1356	arg1	scaffold					1358:1365	This biocompatible nanofiber-reinforced aerogel scaffold	1310:1365	This biocompatible nanofiber-reinforced aerogel scaffold	1310:1365	This biocompatible nanofiber-reinforced aerogel scaffold facilitates osteogenic differentiation by rougher surface, enhanced mechanical strength and well-regulated pores.					
36736519	6	56	theme	cell	804:807	arg1	viability					809:817	Enhanced cell viability	795:817	Enhanced cell viability of the aerogel scaffolds	795:842	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	2	57	theme	fibroin	228:234	arg1	scaffolds					264:272	pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds	218:272	pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%)	218:362	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	9	58	theme	nanofiber-reinforced	1329:1348	arg1	scaffold					1358:1365	This biocompatible nanofiber-reinforced aerogel scaffold	1310:1365	This biocompatible nanofiber-reinforced aerogel scaffold	1310:1365	This biocompatible nanofiber-reinforced aerogel scaffold facilitates osteogenic differentiation by rougher surface, enhanced mechanical strength and well-regulated pores.					
36736519	4	59	theme	%	517:517	arg1	nanofibers					519:528	3 % nanofibers	515:528	3 % nanofibers endowed the aerogels with a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels	515:630	Incorporating 3 % nanofibers endowed the aerogels with a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels.					
36736519	2	60	theme	pure	218:221	arg1	SF					237:238	SF	237:238	SF	237:238	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	2	60	theme	pure	218:221	arg1	fibroin					228:234	pure silk fibroin	218:234	pure silk fibroin (SF)	218:239	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	2	61	theme	silk	223:226	arg1	SF					237:238	SF	237:238	SF	237:238	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	2	61	theme	silk	223:226	arg1	fibroin					228:234	pure silk fibroin	218:234	pure silk fibroin (SF)	218:239	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	2	62	theme	nanofibers	313:322	arg1	nanofibers					313:322	SF nanofibers	310:322	SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%)	310:362	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	2	62	theme	nanofibers	313:322	arg1	amount					300:305	different amount	290:305	different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%)	290:362	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	4	63	theme	3 	515:516	arg1	nanofibers					519:528	3 % nanofibers	515:528	3 % nanofibers endowed the aerogels with a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels	515:630	Incorporating 3 % nanofibers endowed the aerogels with a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels.					
36736519	5	64	theme	nanofibers	649:658	arg1	benefits					637:644	The benefits	633:644	The benefits of nanofibers	633:658	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	7	65	link	enzyme-linked	1095:1107	arg1	ELISA					1130:1134	ELISA	1130:1134	ELISA	1130:1134	The cell differentiation on the aerogel scaffolds was evaluated with enzyme-linked immunosorbent assay (ELISA).					
36736519	7	65	link	enzyme-linked	1095:1107	arg1	assay					1123:1127	enzyme-linked immunosorbent assay	1095:1127	enzyme-linked immunosorbent assay (ELISA)	1095:1135	The cell differentiation on the aerogel scaffolds was evaluated with enzyme-linked immunosorbent assay (ELISA).					
36736519	2	66	theme	SF	310:311	arg1	nanofibers					313:322	SF nanofibers	310:322	SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%)	310:362	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	8	67	theme	phosphatase	1244:1254	arg1	type					1177:1180	Collagen type I	1168:1182	Collagen type I (Col-I)	1168:1190	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	8	67	theme	phosphatase	1244:1254	arg1	expression					1262:1271	alkaline phosphatase (ALP) expression	1235:1271	alkaline phosphatase (ALP) expression	1235:1271	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	4	68	dep	times	576:580	arg1	stress					598:603	the compressive stress	582:603	3.5 times the compressive stress of the pure SF-CS aerogels	572:630	Incorporating 3 % nanofibers endowed the aerogels with a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels.					
36736519	2	69	theme	chitosan	242:249	arg1	scaffolds					264:272	pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds	218:272	pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%)	218:362	Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF1%, SF-CS/NF2% and SF-CS/NF3%) are prepared for bone regeneration.					
36736519	9	70	theme	mechanical	1435:1444	arg1	strength					1446:1453	enhanced mechanical strength	1426:1453	enhanced mechanical strength	1426:1453	This biocompatible nanofiber-reinforced aerogel scaffold facilitates osteogenic differentiation by rougher surface, enhanced mechanical strength and well-regulated pores.					
36736519	8	71	theme	Collagen	1168:1175	arg1	osteocalcin					1193:1203	osteocalcin	1193:1203	osteocalcin (OCN)	1193:1209	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	8	71	theme	Collagen	1168:1175	arg1	type					1177:1180	Collagen type I	1168:1182	Collagen type I (Col-I)	1168:1190	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	8	71	theme	Collagen	1168:1175	arg1	osteopontin					1212:1222	osteopontin	1212:1222	osteopontin (OPN)	1212:1228	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	8	71	theme	Collagen	1168:1175	arg1	Col-I					1185:1189	Col-I	1185:1189	Col-I	1185:1189	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	8	71	theme	Collagen	1168:1175	arg1	expression					1262:1271	alkaline phosphatase (ALP) expression	1235:1271	alkaline phosphatase (ALP) expression	1235:1271	Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF3% aerogels.					
36736519	9	72	theme	enhanced	1426:1433	arg1	strength					1446:1453	enhanced mechanical strength	1426:1453	enhanced mechanical strength	1426:1453	This biocompatible nanofiber-reinforced aerogel scaffold facilitates osteogenic differentiation by rougher surface, enhanced mechanical strength and well-regulated pores.					
36736519	5	73	theme	%	715:715	arg1	porosity					692:699	the high porosity	683:699	the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %	683:792	The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %.					
36736519	9	74	theme	rougher	1409:1415	arg1	surface					1417:1423	rougher surface	1409:1423	rougher surface	1409:1423	This biocompatible nanofiber-reinforced aerogel scaffold facilitates osteogenic differentiation by rougher surface, enhanced mechanical strength and well-regulated pores.					
36736519	3	75	theme	each	485:488	arg1	component					490:498	each component	485:498	each component	485:498	Surface morphology and composition were analyzed to ensure successful integration of each component.					
36736519	4	76	theme	SF-CS	617:621	arg1	aerogels					623:630	the pure SF-CS aerogels	608:630	the pure SF-CS aerogels	608:630	Incorporating 3 % nanofibers endowed the aerogels with a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels.					
36736519	4	77	theme	compressive	586:596	arg1	stress					598:603	the compressive stress	582:603	3.5 times the compressive stress of the pure SF-CS aerogels	572:630	Incorporating 3 % nanofibers endowed the aerogels with a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels.					
36736519	0	78	theme	osteogenic	68:77	arg1	differentiation					79:93	enhanced osteogenic differentiation	59:93	enhanced osteogenic differentiation in MC3T3-E1 cells	59:111	Silk fibroin-chitosan aerogel reinforced by nanofibers for enhanced osteogenic differentiation in MC3T3-E1 cells.					
36736519	6	79	theme	Kit-8	876:880	arg1	assays					890:895	Cell Counting Kit-8 (CCK-8) assays	862:895	Cell Counting Kit-8 (CCK-8) assays	862:895	Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution.					
36736519	7	80	theme	immunosorbent	1109:1121	arg1	ELISA					1130:1134	ELISA	1130:1134	ELISA	1130:1134	The cell differentiation on the aerogel scaffolds was evaluated with enzyme-linked immunosorbent assay (ELISA).					
36736519	7	80	theme	immunosorbent	1109:1121	arg1	assay					1123:1127	enzyme-linked immunosorbent assay	1095:1127	enzyme-linked immunosorbent assay (ELISA)	1095:1135	The cell differentiation on the aerogel scaffolds was evaluated with enzyme-linked immunosorbent assay (ELISA).					
36736519	3	81	theme	component	490:498	arg1	integration					470:480	successful integration	459:480	successful integration of each component	459:498	Surface morphology and composition were analyzed to ensure successful integration of each component.					
36139017	2	0	located	found	299:303	arg2	fungus					240:245	the fungus	236:245	the fungus	236:245	In Estonia, the fungus is well known in the Betula species but can also be found on Alnus.					
36139017	2	0	located	found	299:303	arg1	Alnus					308:312	Alnus	308:312	Alnus	308:312	In Estonia, the fungus is well known in the Betula species but can also be found on Alnus.					
36139017	4	1	from	content	663:669	arg1	conks					697:701	conks	697:701	conks on these hosts	697:716	In the current work, I. obliquus was isolated and, evidently, determined from Alnus incana (L.) Moench., Alnus glutinosa (L.) Gaertn., and Betula pendula Roth, and the content of bioactive compounds in conks on these hosts were analysed.					
36139017	4	1	from	content	663:669	arg1	hosts					712:716	these hosts	706:716	these hosts	706:716	In the current work, I. obliquus was isolated and, evidently, determined from Alnus incana (L.) Moench., Alnus glutinosa (L.) Gaertn., and Betula pendula Roth, and the content of bioactive compounds in conks on these hosts were analysed.					
36139017	8	2	from	incana	1168:1173	arg1	conks					1154:1158	the conks	1150:1158	the conks from A. incana (7455−8961 µg/g) and B. pendula (7881−9057 µg/g)	1150:1222	The content of inotodiol was quite similar in the conks from A. incana (7455−8961 µg/g) and B. pendula (7881−9057 µg/g).					
36139017	4	3	theme	I.	516:517	arg1	obliquus					519:526	I. obliquus	516:526	I. obliquus	516:526	In the current work, I. obliquus was isolated and, evidently, determined from Alnus incana (L.) Moench., Alnus glutinosa (L.) Gaertn., and Betula pendula Roth, and the content of bioactive compounds in conks on these hosts were analysed.					
36139017	0	4	from	Analyses	12:19	arg1	Conks					65:69	Conks	65:69	Conks	65:69	Comparative Analyses of Bioactive Compounds in Inonotus obliquus Conks Growing on Alnus and Betula.					
36139017	3	5	contain	contain	344:350	arg2	compounds					372:380	different bioactive compounds	352:380	different bioactive compounds	352:380	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	3	5	contain	contain	344:350	arg1	conks					323:327	Sterile conks	315:327	Sterile conks of I. obliquus	315:342	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	4	6	theme	Alnus	600:604	arg1	glutinosa					606:614	Alnus glutinosa	600:614	Alnus glutinosa	600:614	In the current work, I. obliquus was isolated and, evidently, determined from Alnus incana (L.) Moench., Alnus glutinosa (L.) Gaertn., and Betula pendula Roth, and the content of bioactive compounds in conks on these hosts were analysed.					
36139017	10	7	theme	I.	1443:1444	arg1	parasitizing					1455:1466	I. obliquus parasitizing	1443:1466	I. obliquus parasitizing	1443:1466	To the best of our knowledge, this study is the first investigation of the chemical composition of I. obliquus parasitizing on Alnus.					
36139017	12	8	theme	I.	1668:1669	arg1	obliquus					1671:1678	I. obliquus	1668:1678	I. obliquus	1668:1678	It supports the opportunity to cultivate I. obliquus, also on the Alnus species, thus increasing the economic value of growing this tree species in forestry.					
36139017	4	9	dep	glutinosa	606:614	arg1	Moench.					591:597	Moench.	591:597	Moench.	591:597	In the current work, I. obliquus was isolated and, evidently, determined from Alnus incana (L.) Moench., Alnus glutinosa (L.) Gaertn., and Betula pendula Roth, and the content of bioactive compounds in conks on these hosts were analysed.					
36139017	11	10	theme	bioactive	1511:1519	arg1	compounds					1521:1529	the bioactive compounds	1507:1529	the bioactive compounds	1507:1529	The results demonstrate that the bioactive compounds are promising in conks of I. obliquus growing not only on Betula but also on the Alnus species.					
36139017	11	10	theme	bioactive	1511:1519	arg1	promising					1535:1543	promising	1535:1543	promising	1535:1543	The results demonstrate that the bioactive compounds are promising in conks of I. obliquus growing not only on Betula but also on the Alnus species.					
36139017	6	11	dep	higher	873:878	arg1	<					865:865	p < 0.05	863:870	p < 0.05	863:870	A significantly (p < 0.05) higher betulinic acid content was found in conks sampled from A. incana when compared with B. pendula: 474−635 and 20−132 µg/g, respectively.					
36139017	10	12	theme	parasitizing	1455:1466	arg1	composition					1428:1438	the chemical composition	1415:1438	the chemical composition of I. obliquus parasitizing	1415:1466	To the best of our knowledge, this study is the first investigation of the chemical composition of I. obliquus parasitizing on Alnus.					
36139017	12	13	theme	economic	1728:1735	arg1	value					1737:1741	the economic value	1724:1741	the economic value of growing this tree species in forestry	1724:1782	It supports the opportunity to cultivate I. obliquus, also on the Alnus species, thus increasing the economic value of growing this tree species in forestry.					
36139017	5	14	dep	159	836:838	arg1	to					833:834	to	833:834	to	833:834	All the analysed conks sampled from A. incana and B. pendula contained betulin that varied from 111 to 159 µg/g.					
36139017	6	15	theme	acid	890:893	arg1	content					895:901	A significantly (p < 0.05) higher betulinic acid content	846:901	A significantly (p < 0.05) higher betulinic acid content	846:901	A significantly (p < 0.05) higher betulinic acid content was found in conks sampled from A. incana when compared with B. pendula: 474−635 and 20−132 µg/g, respectively.					
36139017	5	16	theme	analysed	741:748	arg1	conks					750:754	All the analysed conks	733:754	All the analysed conks sampled from A. incana and B. pendula	733:792	All the analysed conks sampled from A. incana and B. pendula contained betulin that varied from 111 to 159 µg/g.					
36139017	1	17	theme	living	159:164	arg1	species					183:189	some living broadleaved tree species	154:189	some living broadleaved tree species as a pathogen	154:203	Inonotus obliquus grows in the Northern Hemisphere on some living broadleaved tree species as a pathogen, causing stem rot.					
36139017	11	18	from	conks	1548:1552	arg1	compounds					1521:1529	the bioactive compounds	1507:1529	the bioactive compounds	1507:1529	The results demonstrate that the bioactive compounds are promising in conks of I. obliquus growing not only on Betula but also on the Alnus species.					
36139017	11	18	from	conks	1548:1552	arg1	promising					1535:1543	promising	1535:1543	promising	1535:1543	The results demonstrate that the bioactive compounds are promising in conks of I. obliquus growing not only on Betula but also on the Alnus species.					
36139017	3	19	theme	I.	332:333	arg1	obliquus					335:342	I. obliquus	332:342	I. obliquus	332:342	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	1	20	theme	broadleaved	166:176	arg1	species					183:189	some living broadleaved tree species	154:189	some living broadleaved tree species as a pathogen	154:203	Inonotus obliquus grows in the Northern Hemisphere on some living broadleaved tree species as a pathogen, causing stem rot.					
36139017	12	21	theme	Alnus	1693:1697	arg1	species					1699:1705	the Alnus species	1689:1705	the Alnus species	1689:1705	It supports the opportunity to cultivate I. obliquus, also on the Alnus species, thus increasing the economic value of growing this tree species in forestry.					
36139017	4	22	from	hosts	712:716	arg1	content					663:669	the content	659:669	the content of bioactive compounds in conks on these hosts	659:716	In the current work, I. obliquus was isolated and, evidently, determined from Alnus incana (L.) Moench., Alnus glutinosa (L.) Gaertn., and Betula pendula Roth, and the content of bioactive compounds in conks on these hosts were analysed.					
36139017	9	23	theme	tree	1330:1333	arg1	species					1335:1341	these two tree species	1320:1341	these two tree species	1320:1341	Also, no significant differences in the lanosterol content were found between the samples from these two tree species.					
36139017	1	24	theme	tree	178:181	arg1	species					183:189	some living broadleaved tree species	154:189	some living broadleaved tree species as a pathogen	154:203	Inonotus obliquus grows in the Northern Hemisphere on some living broadleaved tree species as a pathogen, causing stem rot.					
36139017	0	25	theme	Comparative	0:10	arg1	Analyses					12:19	Comparative Analyses	0:19	Comparative Analyses of Bioactive Compounds in Inonotus obliquus Conks	0:69	Comparative Analyses of Bioactive Compounds in Inonotus obliquus Conks Growing on Alnus and Betula.					
36139017	2	26	theme	Betula	268:273	arg1	species					275:281	the Betula species	264:281	the Betula species	264:281	In Estonia, the fungus is well known in the Betula species but can also be found on Alnus.					
36139017	0	27	theme	Bioactive	24:32	arg1	Compounds					34:42	Bioactive Compounds	24:42	Bioactive Compounds	24:42	Comparative Analyses of Bioactive Compounds in Inonotus obliquus Conks Growing on Alnus and Betula.					
36139017	11	28	theme	I.	1557:1558	arg1	obliquus					1560:1567	I. obliquus	1557:1567	I. obliquus	1557:1567	The results demonstrate that the bioactive compounds are promising in conks of I. obliquus growing not only on Betula but also on the Alnus species.					
36139017	9	29	from	species	1335:1341	arg1	samples					1307:1313	the samples	1303:1313	the samples from these two tree species	1303:1341	Also, no significant differences in the lanosterol content were found between the samples from these two tree species.					
36139017	7	30	theme	total	1061:1065	arg1	polyphenols					1067:1077	total polyphenols	1061:1077	total polyphenols	1061:1077	However, the conks from Betula were richer in total polyphenols, flavonols, and glucans.					
36139017	3	31	from	conks	451:455	arg1	species					475:481	different host species	460:481	different host species	460:481	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	3	32	theme	comparative	408:418	arg1	research					420:427	the quantitative and comparative research	387:427	the quantitative and comparative research of these compounds in conks on different host species	387:481	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	10	33	theme	first	1392:1396	arg1	investigation					1398:1410	the first investigation	1388:1410	the first investigation of the chemical composition of I. obliquus parasitizing on Alnus	1388:1475	To the best of our knowledge, this study is the first investigation of the chemical composition of I. obliquus parasitizing on Alnus.					
36139017	10	33	theme	first	1392:1396	arg1	study					1379:1383	this study	1374:1383	this study	1374:1383	To the best of our knowledge, this study is the first investigation of the chemical composition of I. obliquus parasitizing on Alnus.					
36139017	10	34	from	investigation	1398:1410	arg1	Alnus					1471:1475	Alnus	1471:1475	Alnus	1471:1475	To the best of our knowledge, this study is the first investigation of the chemical composition of I. obliquus parasitizing on Alnus.					
36139017	1	35	theme	Inonotus	100:107	arg1	obliquus					109:116	Inonotus obliquus	100:116	Inonotus obliquus	100:116	Inonotus obliquus grows in the Northern Hemisphere on some living broadleaved tree species as a pathogen, causing stem rot.					
36139017	8	36	from	pendula	1199:1205	arg1	conks					1154:1158	the conks	1150:1158	the conks from A. incana (7455−8961 µg/g) and B. pendula (7881−9057 µg/g)	1150:1222	The content of inotodiol was quite similar in the conks from A. incana (7455−8961 µg/g) and B. pendula (7881−9057 µg/g).					
36139017	1	37	theme	Northern	131:138	arg1	Hemisphere					140:149	the Northern Hemisphere	127:149	the Northern Hemisphere	127:149	Inonotus obliquus grows in the Northern Hemisphere on some living broadleaved tree species as a pathogen, causing stem rot.					
36139017	3	38	theme	different	352:360	arg1	compounds					372:380	different bioactive compounds	352:380	different bioactive compounds	352:380	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	4	39	theme	current	502:508	arg1	work					510:513	the current work	498:513	the current work	498:513	In the current work, I. obliquus was isolated and, evidently, determined from Alnus incana (L.) Moench., Alnus glutinosa (L.) Gaertn., and Betula pendula Roth, and the content of bioactive compounds in conks on these hosts were analysed.					
36139017	6	40	theme	betulinic	880:888	arg1	content					895:901	A significantly (p < 0.05) higher betulinic acid content	846:901	A significantly (p < 0.05) higher betulinic acid content	846:901	A significantly (p < 0.05) higher betulinic acid content was found in conks sampled from A. incana when compared with B. pendula: 474−635 and 20−132 µg/g, respectively.					
36139017	8	41	from	similar	1139:1145	arg1	conks					1154:1158	the conks	1150:1158	the conks from A. incana (7455−8961 µg/g) and B. pendula (7881−9057 µg/g)	1150:1222	The content of inotodiol was quite similar in the conks from A. incana (7455−8961 µg/g) and B. pendula (7881−9057 µg/g).					
36139017	3	42	theme	quantitative	391:402	arg1	research					420:427	the quantitative and comparative research	387:427	the quantitative and comparative research of these compounds in conks on different host species	387:481	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	0	43	theme	Compounds	34:42	arg1	Analyses					12:19	Comparative Analyses	0:19	Comparative Analyses of Bioactive Compounds in Inonotus obliquus Conks	0:69	Comparative Analyses of Bioactive Compounds in Inonotus obliquus Conks Growing on Alnus and Betula.					
36139017	10	44	theme	chemical	1419:1426	arg1	composition					1428:1438	the chemical composition	1415:1438	the chemical composition of I. obliquus parasitizing	1415:1466	To the best of our knowledge, this study is the first investigation of the chemical composition of I. obliquus parasitizing on Alnus.					
36139017	3	45	theme	bioactive	362:370	arg1	compounds					372:380	different bioactive compounds	352:380	different bioactive compounds	352:380	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	3	46	theme	Sterile	315:321	arg1	conks					323:327	Sterile conks	315:327	Sterile conks of I. obliquus	315:342	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	5	47	contain	contained	794:802	arg2	betulin					804:810	betulin	804:810	betulin that varied from 111 to 159 µg/g	804:843	All the analysed conks sampled from A. incana and B. pendula contained betulin that varied from 111 to 159 µg/g.					
36139017	5	47	contain	contained	794:802	arg1	conks					750:754	All the analysed conks	733:754	All the analysed conks sampled from A. incana and B. pendula	733:792	All the analysed conks sampled from A. incana and B. pendula contained betulin that varied from 111 to 159 µg/g.					
36139017	6	48	theme	higher	873:878	arg1	content					895:901	A significantly (p < 0.05) higher betulinic acid content	846:901	A significantly (p < 0.05) higher betulinic acid content	846:901	A significantly (p < 0.05) higher betulinic acid content was found in conks sampled from A. incana when compared with B. pendula: 474−635 and 20−132 µg/g, respectively.					
36139017	3	49	theme	obliquus	335:342	arg1	conks					323:327	Sterile conks	315:327	Sterile conks of I. obliquus	315:342	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	11	50	theme	Alnus	1612:1616	arg1	species					1618:1624	the Alnus species	1608:1624	the Alnus species	1608:1624	The results demonstrate that the bioactive compounds are promising in conks of I. obliquus growing not only on Betula but also on the Alnus species.					
36139017	9	51	theme	significant	1234:1244	arg1	differences					1246:1256	no significant differences	1231:1256	no significant differences in the lanosterol content	1231:1282	Also, no significant differences in the lanosterol content were found between the samples from these two tree species.					
36139017	9	52	from	differences	1246:1256	arg1	content					1276:1282	the lanosterol content	1261:1282	the lanosterol content	1261:1282	Also, no significant differences in the lanosterol content were found between the samples from these two tree species.					
36139017	3	53	theme	compounds	438:446	arg1	research					420:427	the quantitative and comparative research	387:427	the quantitative and comparative research of these compounds in conks on different host species	387:481	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	11	54	from	promising	1535:1543	arg1	conks					1548:1552	conks	1548:1552	conks of I. obliquus growing not only on Betula but also on the Alnus species	1548:1624	The results demonstrate that the bioactive compounds are promising in conks of I. obliquus growing not only on Betula but also on the Alnus species.					
36139017	11	55	theme	obliquus	1560:1567	arg1	conks					1548:1552	conks	1548:1552	conks of I. obliquus growing not only on Betula but also on the Alnus species	1548:1624	The results demonstrate that the bioactive compounds are promising in conks of I. obliquus growing not only on Betula but also on the Alnus species.					
36139017	4	56	from	conks	697:701	arg1	hosts					712:716	these hosts	706:716	these hosts	706:716	In the current work, I. obliquus was isolated and, evidently, determined from Alnus incana (L.) Moench., Alnus glutinosa (L.) Gaertn., and Betula pendula Roth, and the content of bioactive compounds in conks on these hosts were analysed.					
36139017	10	57	theme	obliquus	1446:1453	arg1	parasitizing					1455:1466	I. obliquus parasitizing	1443:1466	I. obliquus parasitizing	1443:1466	To the best of our knowledge, this study is the first investigation of the chemical composition of I. obliquus parasitizing on Alnus.					
36139017	8	58	from	conks	1154:1158	arg1	similar					1139:1145	similar	1139:1145	similar	1139:1145	The content of inotodiol was quite similar in the conks from A. incana (7455−8961 µg/g) and B. pendula (7881−9057 µg/g).					
36139017	8	58	from	conks	1154:1158	arg1	content					1108:1114	The content	1104:1114	The content of inotodiol	1104:1127	The content of inotodiol was quite similar in the conks from A. incana (7455−8961 µg/g) and B. pendula (7881−9057 µg/g).					
36139017	3	59	from	research	420:427	arg1	species					475:481	different host species	460:481	different host species	460:481	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	3	59	from	research	420:427	arg1	conks					451:455	conks	451:455	conks on different host species	451:481	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	9	60	theme	lanosterol	1265:1274	arg1	content					1276:1282	the lanosterol content	1261:1282	the lanosterol content	1261:1282	Also, no significant differences in the lanosterol content were found between the samples from these two tree species.					
36139017	6	61	located	found	907:911	arg2	content					895:901	A significantly (p < 0.05) higher betulinic acid content	846:901	A significantly (p < 0.05) higher betulinic acid content	846:901	A significantly (p < 0.05) higher betulinic acid content was found in conks sampled from A. incana when compared with B. pendula: 474−635 and 20−132 µg/g, respectively.					
36139017	6	61	located	found	907:911	arg1	conks					916:920	conks	916:920	conks sampled from A. incana when compared with B. pendula: 474−635 and 20−132 µg/g, respectively	916:1012	A significantly (p < 0.05) higher betulinic acid content was found in conks sampled from A. incana when compared with B. pendula: 474−635 and 20−132 µg/g, respectively.					
36139017	7	62	from	Betula	1039:1044	arg1	richer					1051:1056	richer	1051:1056	richer	1051:1056	However, the conks from Betula were richer in total polyphenols, flavonols, and glucans.					
36139017	7	62	from	Betula	1039:1044	arg1	conks					1028:1032	the conks	1024:1032	the conks from Betula	1024:1044	However, the conks from Betula were richer in total polyphenols, flavonols, and glucans.					
36139017	8	63	theme	inotodiol	1119:1127	arg1	similar					1139:1145	similar	1139:1145	similar	1139:1145	The content of inotodiol was quite similar in the conks from A. incana (7455−8961 µg/g) and B. pendula (7881−9057 µg/g).					
36139017	8	63	theme	inotodiol	1119:1127	arg1	content					1108:1114	The content	1104:1114	The content of inotodiol	1104:1127	The content of inotodiol was quite similar in the conks from A. incana (7455−8961 µg/g) and B. pendula (7881−9057 µg/g).					
36139017	12	64	theme	tree	1759:1762	arg1	species					1764:1770	this tree species	1754:1770	this tree species	1754:1770	It supports the opportunity to cultivate I. obliquus, also on the Alnus species, thus increasing the economic value of growing this tree species in forestry.					
36139017	1	65	theme	stem	214:217	arg1	rot					219:221	stem rot	214:221	stem rot	214:221	Inonotus obliquus grows in the Northern Hemisphere on some living broadleaved tree species as a pathogen, causing stem rot.					
36139017	4	66	dep	isolated	532:539	arg1	determined					557:566	determined	557:566	determined from Alnus incana (L.) Moench., Alnus glutinosa (L.) Gaertn., and Betula pendula Roth	557:652	In the current work, I. obliquus was isolated and, evidently, determined from Alnus incana (L.) Moench., Alnus glutinosa (L.) Gaertn., and Betula pendula Roth, and the content of bioactive compounds in conks on these hosts were analysed.					
36139017	4	67	theme	compounds	684:692	arg1	content					663:669	the content	659:669	the content of bioactive compounds in conks on these hosts	659:716	In the current work, I. obliquus was isolated and, evidently, determined from Alnus incana (L.) Moench., Alnus glutinosa (L.) Gaertn., and Betula pendula Roth, and the content of bioactive compounds in conks on these hosts were analysed.					
36139017	10	68	theme	composition	1428:1438	arg1	investigation					1398:1410	the first investigation	1388:1410	the first investigation of the chemical composition of I. obliquus parasitizing on Alnus	1388:1475	To the best of our knowledge, this study is the first investigation of the chemical composition of I. obliquus parasitizing on Alnus.					
36139017	10	68	theme	composition	1428:1438	arg1	study					1379:1383	this study	1374:1383	this study	1374:1383	To the best of our knowledge, this study is the first investigation of the chemical composition of I. obliquus parasitizing on Alnus.					
36139017	3	69	theme	different	460:468	arg1	species					475:481	different host species	460:481	different host species	460:481	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	6	70	dep	pendula	967:973	arg1	µg/g					995:998	474−635 and 20−132 µg/g	976:998	B. pendula: 474−635 and 20−132 µg/g	964:998	A significantly (p < 0.05) higher betulinic acid content was found in conks sampled from A. incana when compared with B. pendula: 474−635 and 20−132 µg/g, respectively.					
36139017	4	71	theme	bioactive	674:682	arg1	compounds					684:692	bioactive compounds	674:692	bioactive compounds	674:692	In the current work, I. obliquus was isolated and, evidently, determined from Alnus incana (L.) Moench., Alnus glutinosa (L.) Gaertn., and Betula pendula Roth, and the content of bioactive compounds in conks on these hosts were analysed.					
36139017	3	72	theme	host	470:473	arg1	species					475:481	different host species	460:481	different host species	460:481	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	3	73	from	species	475:481	arg1	research					420:427	the quantitative and comparative research	387:427	the quantitative and comparative research of these compounds in conks on different host species	387:481	Sterile conks of I. obliquus contain different bioactive compounds, but the quantitative and comparative research of these compounds in conks on different host species is limited.					
36139017	6	74	theme	p	863:863	arg1	<					865:865	p < 0.05	863:870	p < 0.05	863:870	A significantly (p < 0.05) higher betulinic acid content was found in conks sampled from A. incana when compared with B. pendula: 474−635 and 20−132 µg/g, respectively.					
35700659	6	0	theme	yeast	1012:1016	arg1	isolates					1018:1025	yeast isolates	1012:1025	yeast isolates	1012:1025	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	11	1	theme	AD	1812:1813	arg1	lesions					1815:1821	AD lesions	1812:1821	AD lesions	1812:1821	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	3	2	theme	healthy	535:541	arg1	subjects					543:550	healthy subjects	535:550	healthy subjects according to the culture methods used for Malassezia species isolation	535:621	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	3	3	theme	atopic	445:450	arg1	AD					464:465	AD	464:465	AD	464:465	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	3	3	theme	atopic	445:450	arg1	dermatitis					452:461	atopic dermatitis	445:461	atopic dermatitis (AD)	445:466	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	2	4	theme	various	278:284	arg1	conditions					335:344	various Malassezia-caused or Malassezia exacerbated skin conditions	278:344	various Malassezia-caused or Malassezia exacerbated skin conditions	278:344	There are complex interactions between this commensal and the skin, leading to various Malassezia-caused or Malassezia exacerbated skin conditions.					
35700659	1	5	theme	flora	192:196	arg1	component					160:168	the dominant eukaryotic component	136:168	the dominant eukaryotic component of the skin microbial flora	136:196	BACKGROUND The genus Malassezia represents the dominant eukaryotic component of the skin microbial flora.					
35700659	6	6	theme	microscopic	813:823	arg1	examination					825:835	Direct microscopic examination	806:835	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan	806:974	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	10	7	from	prevalent	1479:1487	arg1	lesions					1495:1501	PV lesions	1492:1501	PV lesions	1492:1501	Malassezia species isolation was found to be most prevalent in PV lesions, with M. furfur being the most prevalent identified species (52.5 %), followed by unidentified species (20%).					
35700659	3	8	with	patients	403:410	arg1	versicolor					428:437	pityriasis versicolor	417:437	pityriasis versicolor (PV)	417:442	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	3	8	with	patients	403:410	arg1	dermatitis					452:461	atopic dermatitis	445:461	atopic dermatitis (AD)	445:466	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	3	8	with	patients	403:410	arg1	dermatitis					484:493	seborrheic dermatitis	473:493	seborrheic dermatitis (SD)	473:498	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	3	8	with	patients	403:410	arg1	AD					464:465	AD	464:465	AD	464:465	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	3	8	with	patients	403:410	arg1	SD					496:497	SD	496:497	SD	496:497	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	3	8	with	patients	403:410	arg1	PV					440:441	PV	440:441	PV	440:441	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	11	9	from	SD	1675:1676	arg1	similar					1660:1666	similar	1660:1666	similar	1660:1666	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	3	10	from	species	381:387	arg1	lesions					392:398	lesions	392:398	lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD)	392:498	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	3	10	from	species	381:387	arg1	subjects					543:550	healthy subjects	535:550	healthy subjects according to the culture methods used for Malassezia species isolation	535:621	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	2	11	theme	exacerbated	318:328	arg1	conditions					335:344	various Malassezia-caused or Malassezia exacerbated skin conditions	278:344	various Malassezia-caused or Malassezia exacerbated skin conditions	278:344	There are complex interactions between this commensal and the skin, leading to various Malassezia-caused or Malassezia exacerbated skin conditions.					
35700659	11	12	theme	Malassezia	1751:1760	arg1	species					1792:1798	the prevalent species	1778:1798	the prevalent species isolated in AD lesions	1778:1821	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	11	12	theme	Malassezia	1751:1760	arg1	sympodialis					1762:1772	Malassezia sympodialis	1751:1772	Malassezia sympodialis	1751:1772	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	12	13	theme	Chrome	1824:1829	arg1	media					1836:1840	Chrome agar media	1824:1840	Chrome agar media	1824:1840	Chrome agar media can be promising for the identification of Malassezia species phenotypically.					
35700659	7	14	theme	healthy	1085:1091	arg1	subjects					1093:1100	healthy subjects	1085:1100	healthy subjects	1085:1100	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35700659	5	15	theme	Malt	787:790	arg1	agar					800:803	Malt extract agar	787:803	Malt extract agar	787:803	For 10-14 days, specimens were cultured on Dixon's medium and Malt extract agar.					
35700659	6	16	theme	chrome	873:878	arg1	agar					880:883	Hi chrome agar	870:883	Hi chrome agar	870:883	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	11	17	from	similar	1660:1666	arg1	subjects					1691:1698	healthy subjects	1683:1698	healthy subjects	1683:1698	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	11	17	from	similar	1660:1666	arg1	SD					1675:1676	SD	1675:1676	SD	1675:1676	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	11	17	from	similar	1660:1666	arg1	PV					1671:1672	PV	1671:1672	PV	1671:1672	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	11	18	theme	prevalent	1782:1790	arg1	species					1792:1798	the prevalent species	1778:1798	the prevalent species isolated in AD lesions	1778:1821	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	11	18	theme	prevalent	1782:1790	arg1	sympodialis					1762:1772	Malassezia sympodialis	1751:1772	Malassezia sympodialis	1751:1772	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	8	19	theme	M.	1298:1299	arg1	globosa					1301:1307	M. globosa	1298:1307	M. globosa (10%)	1298:1313	In patients with SD, M. furfur was isolated more frequently from scalp lesions (25.0%) and then M. sympodialis (15%) and M. globosa (10%).					
35700659	8	19	theme	M.	1298:1299	arg1	%					1312:1312	10%	1310:1312	10%	1310:1312	In patients with SD, M. furfur was isolated more frequently from scalp lesions (25.0%) and then M. sympodialis (15%) and M. globosa (10%).					
35700659	11	20	from	PV	1671:1672	arg1	similar					1660:1666	similar	1660:1666	similar	1660:1666	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	9	21	theme	Malassezia	1316:1325	arg1	species					1371:1377	the most prevalent isolated species	1343:1377	the most prevalent isolated species	1343:1377	Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%).					
35700659	9	21	theme	Malassezia	1316:1325	arg1	sympodialis					1327:1337	Malassezia sympodialis	1316:1337	Malassezia sympodialis	1316:1337	Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%).					
35700659	0	22	theme	associated	67:76	arg1	diseases					83:90	some Malassezia associated skin diseases	51:90	some Malassezia associated skin diseases	51:90	Identification of Malassezia species isolated from some Malassezia associated skin diseases.					
35700659	3	23	theme	species	605:611	arg1	isolation					613:621	Malassezia species isolation	594:621	Malassezia species isolation	594:621	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	9	24	from	species	1371:1377	arg1	lesions					1385:1391	AD lesions	1382:1391	AD lesions (20%)	1382:1397	Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%).					
35700659	9	24	from	species	1371:1377	arg1	%					1396:1396	20%	1394:1396	20%	1394:1396	Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%).					
35700659	6	25	theme	Tweens	926:931	arg1	assimilation					933:944	Tweens assimilation	926:944	Tweens assimilation	926:944	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	6	26	from	assimilation	933:944	arg1	agar					880:883	Hi chrome agar	870:883	Hi chrome agar	870:883	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	1	27	dep	BACKGROUND	93:102	arg1	represents					125:134	represents	125:134	represents the dominant eukaryotic component of the skin microbial flora	125:196	BACKGROUND The genus Malassezia represents the dominant eukaryotic component of the skin microbial flora.					
35700659	3	28	from	sites	526:530	arg1	lesions					392:398	lesions	392:398	lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD)	392:498	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	3	28	from	sites	526:530	arg1	subjects					543:550	healthy subjects	535:550	healthy subjects according to the culture methods used for Malassezia species isolation	535:621	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	1	29	theme	skin	177:180	arg1	flora					192:196	the skin microbial flora	173:196	the skin microbial flora	173:196	BACKGROUND The genus Malassezia represents the dominant eukaryotic component of the skin microbial flora.					
35700659	10	30	theme	M.	1509:1510	arg1	furfur					1512:1517	M. furfur	1509:1517	M. furfur	1509:1517	Malassezia species isolation was found to be most prevalent in PV lesions, with M. furfur being the most prevalent identified species (52.5 %), followed by unidentified species (20%).					
35700659	10	30	theme	M.	1509:1510	arg1	prevalent					1534:1542	prevalent	1534:1542	prevalent	1534:1542	Malassezia species isolation was found to be most prevalent in PV lesions, with M. furfur being the most prevalent identified species (52.5 %), followed by unidentified species (20%).					
35700659	9	31	theme	isolated	1362:1369	arg1	species					1371:1377	the most prevalent isolated species	1343:1377	the most prevalent isolated species	1343:1377	Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%).					
35700659	9	31	theme	isolated	1362:1369	arg1	sympodialis					1327:1337	Malassezia sympodialis	1316:1337	Malassezia sympodialis	1316:1337	Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%).					
35700659	1	32	theme	eukaryotic	149:158	arg1	component					160:168	the dominant eukaryotic component	136:168	the dominant eukaryotic component of the skin microbial flora	136:196	BACKGROUND The genus Malassezia represents the dominant eukaryotic component of the skin microbial flora.					
35700659	11	33	theme	species	1636:1642	arg1	composition					1644:1654	CONCLUSIONS Malassezia species composition	1613:1654	CONCLUSIONS Malassezia species composition	1613:1654	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	6	34	used	used	981:984	arg2	examination					825:835	Direct microscopic examination	806:835	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan	806:974	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	3	35	theme	pityriasis	417:426	arg1	PV					440:441	PV	440:441	PV	440:441	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	3	35	theme	pityriasis	417:426	arg1	versicolor					428:437	pityriasis versicolor	417:437	pityriasis versicolor (PV)	417:442	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	8	36	theme	scalp	1242:1246	arg1	lesions					1248:1254	scalp lesions	1242:1254	scalp lesions (25.0%)	1242:1262	In patients with SD, M. furfur was isolated more frequently from scalp lesions (25.0%) and then M. sympodialis (15%) and M. globosa (10%).					
35700659	8	36	theme	scalp	1242:1246	arg1	%					1261:1261	25.0%	1257:1261	25.0%	1257:1261	In patients with SD, M. furfur was isolated more frequently from scalp lesions (25.0%) and then M. sympodialis (15%) and M. globosa (10%).					
35700659	11	37	theme	CONCLUSIONS	1613:1623	arg1	species					1636:1642	CONCLUSIONS Malassezia species	1613:1642	CONCLUSIONS Malassezia species composition	1613:1654	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	3	38	theme	patients	403:410	arg1	lesions					392:398	lesions	392:398	lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD)	392:498	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	3	39	theme	Malassezia	594:603	arg1	isolation					613:621	Malassezia species isolation	594:621	Malassezia species isolation	594:621	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	0	40	theme	Malassezia	18:27	arg1	species					29:35	Malassezia species	18:35	Malassezia species	18:35	Identification of Malassezia species isolated from some Malassezia associated skin diseases.					
35700659	3	41	theme	corresponding	512:524	arg1	sites					526:530	corresponding sites	512:530	corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation	512:621	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	6	42	theme	Direct	806:811	arg1	examination					825:835	Direct microscopic examination	806:835	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan	806:974	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	6	43	theme	tryptophan	965:974	arg1	stain					849:853	Gram's stain	842:853	Gram's stain	842:853	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	6	43	theme	tryptophan	965:974	arg1	hydrolysis					951:960	hydrolysis	951:960	hydrolysis of tryptophan	951:974	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	6	43	theme	tryptophan	965:974	arg1	medium					894:899	Dixon's medium	886:899	Dixon's medium at various temperatures	886:923	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	6	43	theme	tryptophan	965:974	arg1	assimilation					933:944	Tweens assimilation	926:944	Tweens assimilation	926:944	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	6	43	theme	tryptophan	965:974	arg1	subculture					856:865	subculture	856:865	subculture on Hi chrome agar	856:883	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	11	44	from	subjects	1691:1698	arg1	similar					1660:1666	similar	1660:1666	similar	1660:1666	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	6	45	from	medium	894:899	arg1	agar					880:883	Hi chrome agar	870:883	Hi chrome agar	870:883	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	8	46	with	patients	1180:1187	arg1	SD					1194:1195	SD	1194:1195	SD	1194:1195	In patients with SD, M. furfur was isolated more frequently from scalp lesions (25.0%) and then M. sympodialis (15%) and M. globosa (10%).					
35700659	12	47	theme	Malassezia	1885:1894	arg1	species					1896:1902	Malassezia species	1885:1902	Malassezia species	1885:1902	Chrome agar media can be promising for the identification of Malassezia species phenotypically.					
35700659	10	48	theme	species	1440:1446	arg1	isolation					1448:1456	Malassezia species isolation	1429:1456	Malassezia species isolation was found to be most prevalent in PV lesions, with M. furfur being the most prevalent	1429:1542	Malassezia species isolation was found to be most prevalent in PV lesions, with M. furfur being the most prevalent identified species (52.5 %), followed by unidentified species (20%).					
35700659	5	49	theme	extract	792:798	arg1	agar					800:803	Malt extract agar	787:803	Malt extract agar	787:803	For 10-14 days, specimens were cultured on Dixon's medium and Malt extract agar.					
35700659	8	50	attach	isolated	1212:1219	arg2	furfur					1201:1206	M. furfur	1198:1206	M. furfur	1198:1206	In patients with SD, M. furfur was isolated more frequently from scalp lesions (25.0%) and then M. sympodialis (15%) and M. globosa (10%).					
35700659	8	50	attach	isolated	1212:1219	arg1	lesions					1248:1254	scalp lesions	1242:1254	scalp lesions (25.0%)	1242:1262	In patients with SD, M. furfur was isolated more frequently from scalp lesions (25.0%) and then M. sympodialis (15%) and M. globosa (10%).					
35700659	8	50	attach	isolated	1212:1219	arg1	%					1261:1261	25.0%	1257:1261	25.0%	1257:1261	In patients with SD, M. furfur was isolated more frequently from scalp lesions (25.0%) and then M. sympodialis (15%) and M. globosa (10%).					
35700659	7	51	theme	Malassezia	1063:1072	arg1	species					1074:1080	Malassezia species	1063:1080	Malassezia species	1063:1080	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35700659	7	52	from	frequency	1050:1058	arg1	subjects					1093:1100	healthy subjects	1085:1100	healthy subjects	1085:1100	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35700659	8	53	theme	M.	1273:1274	arg1	sympodialis					1276:1286	M. sympodialis	1273:1286	M. sympodialis (15%)	1273:1292	In patients with SD, M. furfur was isolated more frequently from scalp lesions (25.0%) and then M. sympodialis (15%) and M. globosa (10%).					
35700659	8	53	theme	M.	1273:1274	arg1	%					1291:1291	15%	1289:1291	15%	1289:1291	In patients with SD, M. furfur was isolated more frequently from scalp lesions (25.0%) and then M. sympodialis (15%) and M. globosa (10%).					
35700659	9	54	theme	M.	1412:1413	arg1	furfur					1415:1420	M. furfur	1412:1420	M. furfur (10%)	1412:1426	Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%).					
35700659	9	54	theme	M.	1412:1413	arg1	%					1425:1425	10%	1423:1425	10%	1423:1425	Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%).					
35700659	6	55	from	stain	849:853	arg1	agar					880:883	Hi chrome agar	870:883	Hi chrome agar	870:883	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	6	56	theme	isolates	1018:1025	arg1	identification					994:1007	the identification	990:1007	the identification of yeast isolates	990:1025	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	6	57	from	subculture	856:865	arg1	agar					880:883	Hi chrome agar	870:883	Hi chrome agar	870:883	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	7	58	theme	isolation	1040:1048	arg1	%					1110:1110	13.3%	1106:1110	13.3% for M. furfur	1106:1124	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35700659	7	58	theme	isolation	1040:1048	arg1	frequency					1050:1058	The isolation frequency	1036:1058	The isolation frequency of Malassezia species in healthy subjects	1036:1100	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35700659	2	59	theme	Malassezia-caused	286:302	arg1	conditions					335:344	various Malassezia-caused or Malassezia exacerbated skin conditions	278:344	various Malassezia-caused or Malassezia exacerbated skin conditions	278:344	There are complex interactions between this commensal and the skin, leading to various Malassezia-caused or Malassezia exacerbated skin conditions.					
35700659	1	60	theme	microbial	182:190	arg1	flora					192:196	the skin microbial flora	173:196	the skin microbial flora	173:196	BACKGROUND The genus Malassezia represents the dominant eukaryotic component of the skin microbial flora.					
35700659	12	61	theme	agar	1831:1834	arg1	media					1836:1840	Chrome agar media	1824:1840	Chrome agar media	1824:1840	Chrome agar media can be promising for the identification of Malassezia species phenotypically.					
35700659	7	62	dep	RESULTS	1028:1034	arg1	%					1156:1156	3.3%	1153:1156	3.3% for M.sympodialis	1153:1174	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35700659	7	62	dep	RESULTS	1028:1034	arg1	%					1110:1110	13.3%	1106:1110	13.3% for M. furfur	1106:1124	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35700659	7	62	dep	RESULTS	1028:1034	arg1	%					1131:1131	10.0%	1127:1131	10.0% for M. globosa	1127:1146	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35700659	7	62	dep	RESULTS	1028:1034	arg1	frequency					1050:1058	The isolation frequency	1036:1058	The isolation frequency of Malassezia species in healthy subjects	1036:1100	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35700659	6	63	from	hydrolysis	951:960	arg1	agar					880:883	Hi chrome agar	870:883	Hi chrome agar	870:883	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	10	64	from	lesions	1495:1501	arg1	prevalent					1479:1487	prevalent	1479:1487	prevalent	1479:1487	Malassezia species isolation was found to be most prevalent in PV lesions, with M. furfur being the most prevalent identified species (52.5 %), followed by unidentified species (20%).					
35700659	2	65	theme	skin	330:333	arg1	conditions					335:344	various Malassezia-caused or Malassezia exacerbated skin conditions	278:344	various Malassezia-caused or Malassezia exacerbated skin conditions	278:344	There are complex interactions between this commensal and the skin, leading to various Malassezia-caused or Malassezia exacerbated skin conditions.					
35700659	4	66	theme	METHODS	624:630	arg1	Scrapings					632:640	METHODS Scrapings	624:640	METHODS Scrapings	624:640	METHODS Scrapings were collected from 80 patients (40 PV, 20 AD, and 20 SD) and 30 healthy subjects.					
35700659	3	67	theme	culture	569:575	arg1	methods					577:583	the culture methods	565:583	the culture methods used for Malassezia species isolation	565:621	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	6	68	theme	Hi	870:871	arg1	agar					880:883	Hi chrome agar	870:883	Hi chrome agar	870:883	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	1	69	theme	dominant	140:147	arg1	component					160:168	the dominant eukaryotic component	136:168	the dominant eukaryotic component of the skin microbial flora	136:196	BACKGROUND The genus Malassezia represents the dominant eukaryotic component of the skin microbial flora.					
35700659	11	70	theme	commonest	1726:1734	arg1	isolate					1736:1742	the commonest isolate	1722:1742	the commonest isolate	1722:1742	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	11	70	theme	commonest	1726:1734	arg1	furfur					1709:1714	furfur	1709:1714	furfur	1709:1714	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	0	71	theme	skin	78:81	arg1	diseases					83:90	some Malassezia associated skin diseases	51:90	some Malassezia associated skin diseases	51:90	Identification of Malassezia species isolated from some Malassezia associated skin diseases.					
35700659	11	72	theme	healthy	1683:1689	arg1	subjects					1691:1698	healthy subjects	1683:1698	healthy subjects	1683:1698	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	3	73	theme	Malassezia	370:379	arg1	species					381:387	Malassezia species	370:387	Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD)	370:498	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	13	74	theme	molecular	1979:1987	arg1	methods					1989:1995	molecular methods	1979:1995	molecular methods	1979:1995	However, species differentiation has to be complemented by molecular methods.					
35700659	8	75	theme	M.	1198:1199	arg1	furfur					1201:1206	M. furfur	1198:1206	M. furfur	1198:1206	In patients with SD, M. furfur was isolated more frequently from scalp lesions (25.0%) and then M. sympodialis (15%) and M. globosa (10%).					
35700659	7	76	theme	M.	1116:1117	arg1	furfur					1119:1124	M. furfur	1116:1124	M. furfur	1116:1124	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35700659	4	77	dep	patients	665:672	arg1	SD					696:697	20 SD	693:697	20 SD	693:697	METHODS Scrapings were collected from 80 patients (40 PV, 20 AD, and 20 SD) and 30 healthy subjects.					
35700659	4	77	dep	patients	665:672	arg1	patients					665:672	80 patients	662:672	80 patients (40 PV, 20 AD, and 20 SD)	662:698	METHODS Scrapings were collected from 80 patients (40 PV, 20 AD, and 20 SD) and 30 healthy subjects.					
35700659	4	77	dep	patients	665:672	arg1	PV					678:679	40 PV	675:679	40 PV	675:679	METHODS Scrapings were collected from 80 patients (40 PV, 20 AD, and 20 SD) and 30 healthy subjects.					
35700659	4	77	dep	patients	665:672	arg1	AD					685:686	20 AD	682:686	20 AD	682:686	METHODS Scrapings were collected from 80 patients (40 PV, 20 AD, and 20 SD) and 30 healthy subjects.					
35700659	9	78	theme	prevalent	1352:1360	arg1	species					1371:1377	the most prevalent isolated species	1343:1377	the most prevalent isolated species	1343:1377	Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%).					
35700659	9	78	theme	prevalent	1352:1360	arg1	sympodialis					1327:1337	Malassezia sympodialis	1316:1337	Malassezia sympodialis	1316:1337	Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%).					
35700659	6	79	theme	various	904:910	arg1	temperatures					912:923	various temperatures	904:923	various temperatures	904:923	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	4	80	theme	healthy	707:713	arg1	subjects					715:722	30 healthy subjects	704:722	30 healthy subjects	704:722	METHODS Scrapings were collected from 80 patients (40 PV, 20 AD, and 20 SD) and 30 healthy subjects.					
35700659	9	81	theme	AD	1382:1383	arg1	lesions					1385:1391	AD lesions	1382:1391	AD lesions (20%)	1382:1397	Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%).					
35700659	9	81	theme	AD	1382:1383	arg1	%					1396:1396	20%	1394:1396	20%	1394:1396	Malassezia sympodialis was the most prevalent isolated species in AD lesions (20%), followed by M. furfur (10%).					
35700659	6	82	from	temperatures	912:923	arg1	stain					849:853	Gram's stain	842:853	Gram's stain	842:853	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	6	82	from	temperatures	912:923	arg1	hydrolysis					951:960	hydrolysis	951:960	hydrolysis of tryptophan	951:974	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	6	82	from	temperatures	912:923	arg1	medium					894:899	Dixon's medium	886:899	Dixon's medium at various temperatures	886:923	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	6	82	from	temperatures	912:923	arg1	assimilation					933:944	Tweens assimilation	926:944	Tweens assimilation	926:944	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	6	82	from	temperatures	912:923	arg1	subculture					856:865	subculture	856:865	subculture on Hi chrome agar	856:883	Direct microscopic examination with Gram's stain, subculture on Hi chrome agar, Dixon's medium at various temperatures, Tweens assimilation, and hydrolysis of tryptophan were used for the identification of yeast isolates.					
35700659	11	83	theme	Malassezia	1625:1634	arg1	species					1636:1642	CONCLUSIONS Malassezia species	1613:1642	CONCLUSIONS Malassezia species composition	1613:1654	CONCLUSIONS Malassezia species composition was similar in PV, SD, and healthy subjects, with M. furfur being the commonest isolate, while Malassezia sympodialis was the prevalent species isolated in AD lesions.					
35700659	0	84	theme	species	29:35	arg1	Identification					0:13	Identification	0:13	Identification of Malassezia species	0:35	Identification of Malassezia species isolated from some Malassezia associated skin diseases.					
35700659	1	85	theme	genus	108:112	arg1	Malassezia					114:123	The genus Malassezia	104:123	The genus Malassezia	104:123	BACKGROUND The genus Malassezia represents the dominant eukaryotic component of the skin microbial flora.					
35700659	3	86	theme	seborrheic	473:482	arg1	SD					496:497	SD	496:497	SD	496:497	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	3	86	theme	seborrheic	473:482	arg1	dermatitis					484:493	seborrheic dermatitis	473:493	seborrheic dermatitis (SD)	473:498	OBJECTIVES To identify Malassezia species in lesions of patients with pityriasis versicolor (PV), atopic dermatitis (AD), and seborrheic dermatitis (SD), as well as corresponding sites in healthy subjects according to the culture methods used for Malassezia species isolation.					
35700659	7	87	theme	species	1074:1080	arg1	%					1110:1110	13.3%	1106:1110	13.3% for M. furfur	1106:1124	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35700659	7	87	theme	species	1074:1080	arg1	frequency					1050:1058	The isolation frequency	1036:1058	The isolation frequency of Malassezia species in healthy subjects	1036:1100	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35700659	10	88	theme	unidentified	1585:1596	arg1	species					1598:1604	unidentified species	1585:1604	unidentified species (20%)	1585:1610	Malassezia species isolation was found to be most prevalent in PV lesions, with M. furfur being the most prevalent identified species (52.5 %), followed by unidentified species (20%).					
35700659	10	88	theme	unidentified	1585:1596	arg1	%					1609:1609	20%	1607:1609	20%	1607:1609	Malassezia species isolation was found to be most prevalent in PV lesions, with M. furfur being the most prevalent identified species (52.5 %), followed by unidentified species (20%).					
35700659	10	89	theme	Malassezia	1429:1438	arg1	isolation					1448:1456	Malassezia species isolation	1429:1456	Malassezia species isolation was found to be most prevalent in PV lesions, with M. furfur being the most prevalent	1429:1542	Malassezia species isolation was found to be most prevalent in PV lesions, with M. furfur being the most prevalent identified species (52.5 %), followed by unidentified species (20%).					
35700659	0	90	theme	Malassezia	56:65	arg1	diseases					83:90	some Malassezia associated skin diseases	51:90	some Malassezia associated skin diseases	51:90	Identification of Malassezia species isolated from some Malassezia associated skin diseases.					
35700659	12	91	theme	species	1896:1902	arg1	identification					1867:1880	the identification	1863:1880	the identification of Malassezia species	1863:1902	Chrome agar media can be promising for the identification of Malassezia species phenotypically.					
35700659	13	92	theme	species	1929:1935	arg1	differentiation					1937:1951	species differentiation	1929:1951	species differentiation	1929:1951	However, species differentiation has to be complemented by molecular methods.					
35700659	2	93	theme	complex	209:215	arg1	interactions					217:228	complex interactions	209:228	complex interactions between this commensal and the skin	209:264	There are complex interactions between this commensal and the skin, leading to various Malassezia-caused or Malassezia exacerbated skin conditions.					
35700659	10	94	theme	PV	1492:1493	arg1	lesions					1495:1501	PV lesions	1492:1501	PV lesions	1492:1501	Malassezia species isolation was found to be most prevalent in PV lesions, with M. furfur being the most prevalent identified species (52.5 %), followed by unidentified species (20%).					
35700659	7	95	theme	M.	1137:1138	arg1	globosa					1140:1146	M. globosa	1137:1146	M. globosa	1137:1146	RESULTS The isolation frequency of Malassezia species in healthy subjects was 13.3% for M. furfur, 10.0% for M. globosa, and 3.3% for M.sympodialis.					
35882879	7	0	theme	adsorption	949:958	arg1	capacity					960:967	the maximum adsorption capacity	937:967	the maximum adsorption capacity	937:967	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	7	0	theme	adsorption	949:958	arg1	659 mg g-1					973:982	659 mg g-1	973:982	659 mg g-1	973:982	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	1	1	theme	Adsorption	162:171	arg1	efficiency					173:182	Adsorption efficiency	162:182	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2)	162:333	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	10	2	theme	nanocomposites	1163:1176	arg1	beads					1178:1182	The nanocomposites beads	1159:1182	The nanocomposites beads	1159:1182	The nanocomposites beads also exhibit excellent reproducibility after ten adsorption cycles.					
35882879	0	3	theme	sulfated	74:81	arg1	zirconia					83:90	nano sulfated zirconia	69:90	nano sulfated zirconia	69:90	Development of hybrid green nanocomposite polymeric beads doped with nano sulfated zirconia for effective removal of Cefotaxime antibiotic from aqueous solution.					
35882879	5	4	from	3.58 h	758:763	arg1	concentration					736:748	88.97 mg L-1 initial cefotaxime concentration	704:748	88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads	704:786	The ideal conditions to achieve complete removal are 88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads composite with 2.5 wt.					
35882879	2	5	dep	embedded	440:447	arg1	technique					425:433	solvent-free and easy calcination technique	391:433	solvent-free and easy calcination technique	391:433	SZrO2 was synthesized from solvent-free and easy calcination technique then embedded with 1-2.5 wt.					
35882879	9	6	theme	SPVA	1139:1142	arg1	IC					1145:1146	IC	1145:1146	IC	1145:1146	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	6	theme	SPVA	1139:1142	arg1	SPVA					1139:1142	SPVA	1139:1142	SPVA	1139:1142	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	6	theme	SPVA	1139:1142	arg1	groups					1129:1134	the different functional groups	1104:1134	the different functional groups of SPVA, IC and SZrO2	1104:1156	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	6	theme	SPVA	1139:1142	arg1	SZrO2					1152:1156	SZrO2	1152:1156	SZrO2	1152:1156	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	7	7	with	composite	992:1000	arg1	wt					1011:1012	2.5 wt	1007:1012	2.5 wt	1007:1012	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	7	8	theme	second	833:838	arg1	kinetics					846:853	The pseudo second order kinetics	822:853	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads	822:930	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	7	8	theme	second	833:838	arg1	659 mg g-1					973:982	659 mg g-1	973:982	659 mg g-1	973:982	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	0	9	theme	effective	96:104	arg1	removal					106:112	effective removal	96:112	effective removal of Cefotaxime antibiotic from aqueous solution	96:159	Development of hybrid green nanocomposite polymeric beads doped with nano sulfated zirconia for effective removal of Cefotaxime antibiotic from aqueous solution.					
35882879	7	10	theme	nanocomposite	912:924	arg1	beads					926:930	nanocomposite beads	912:930	nanocomposite beads	912:930	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	7	11	theme	order	840:844	arg1	kinetics					846:853	The pseudo second order kinetics	822:853	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads	822:930	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	7	11	theme	order	840:844	arg1	659 mg g-1					973:982	659 mg g-1	973:982	659 mg g-1	973:982	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	4	12	theme	dosage	568:573	arg1	effects					557:563	the effects	553:563	the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption	553:648	A batch adsorption experiment was carried out to investigate the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption.					
35882879	5	13	theme	88.97 mg	704:711	arg1	concentration					736:748	88.97 mg L-1 initial cefotaxime concentration	704:748	88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads	704:786	The ideal conditions to achieve complete removal are 88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads composite with 2.5 wt.					
35882879	9	14	theme	IC	1145:1146	arg1	IC					1145:1146	IC	1145:1146	IC	1145:1146	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	14	theme	IC	1145:1146	arg1	SPVA					1139:1142	SPVA	1139:1142	SPVA	1139:1142	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	14	theme	IC	1145:1146	arg1	groups					1129:1134	the different functional groups	1104:1134	the different functional groups of SPVA, IC and SZrO2	1104:1156	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	14	theme	IC	1145:1146	arg1	SZrO2					1152:1156	SZrO2	1152:1156	SZrO2	1152:1156	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	3	15	theme	polymeric	474:482	arg1	matrix					484:489	the polymeric matrix	470:489	the polymeric matrix	470:489	% into the polymeric matrix.					
35882879	1	16	theme	poly	274:277	arg1	SPVA					294:297	SPVA	294:297	SPVA	294:297	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	1	16	theme	poly	274:277	arg1	alcohol					285:291	poly vinyl alcohol	274:291	poly vinyl alcohol (SPVA)	274:298	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	4	17	from	effects	557:563	arg1	adsorption					639:648	Cefotaxime antibiotic adsorption	617:648	Cefotaxime antibiotic adsorption	617:648	A batch adsorption experiment was carried out to investigate the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption.					
35882879	7	18	from	adsorption	884:893	arg1	beads					926:930	nanocomposite beads	912:930	nanocomposite beads	912:930	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	4	19	theme	concentration	590:602	arg1	effects					557:563	the effects	553:563	the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption	553:648	A batch adsorption experiment was carried out to investigate the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption.					
35882879	5	20	with	3.58 h	758:763	arg1	11.68 mg					770:777	11.68 mg	770:777	11.68 mg of beads	770:786	The ideal conditions to achieve complete removal are 88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads composite with 2.5 wt.					
35882879	7	21	theme	pseudo	826:831	arg1	kinetics					846:853	The pseudo second order kinetics	822:853	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads	822:930	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	7	21	theme	pseudo	826:831	arg1	659 mg g-1					973:982	659 mg g-1	973:982	659 mg g-1	973:982	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	4	22	theme	Cefotaxime	617:626	arg1	adsorption					639:648	Cefotaxime antibiotic adsorption	617:648	Cefotaxime antibiotic adsorption	617:648	A batch adsorption experiment was carried out to investigate the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption.					
35882879	0	23	from	solution	152:159	arg1	antibiotic					128:137	antibiotic	128:137	antibiotic	128:137	Development of hybrid green nanocomposite polymeric beads doped with nano sulfated zirconia for effective removal of Cefotaxime antibiotic from aqueous solution.					
35882879	7	24	dep	model	855:859	arg1	illustrated					868:878	illustrated	868:878	model better illustrated the adsorption of cefotaxime on nanocomposite beads	855:930	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	9	25	theme	SZrO2	1152:1156	arg1	IC					1145:1146	IC	1145:1146	IC	1145:1146	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	25	theme	SZrO2	1152:1156	arg1	SPVA					1139:1142	SPVA	1139:1142	SPVA	1139:1142	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	25	theme	SZrO2	1152:1156	arg1	groups					1129:1134	the different functional groups	1104:1134	the different functional groups of SPVA, IC and SZrO2	1104:1156	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	25	theme	SZrO2	1152:1156	arg1	SZrO2					1152:1156	SZrO2	1152:1156	SZrO2	1152:1156	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	11	26	theme	nanocomposite	1371:1383	arg1	beads					1385:1389	this novel nanocomposite beads	1360:1389	this novel nanocomposite beads has strong potential in water treatment for the antibiotic contaminant removal	1360:1468	This type of nanocomposites beads can be easily separated from water without leaving any residue, verifying this novel nanocomposite beads has strong potential in water treatment for the antibiotic contaminant removal.					
35882879	1	27	theme	Cefotaxime	187:196	arg1	efficiency					173:182	Adsorption efficiency	162:182	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2)	162:333	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	5	28	theme	beads	782:786	arg1	11.68 mg					770:777	11.68 mg	770:777	11.68 mg of beads	770:786	The ideal conditions to achieve complete removal are 88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads composite with 2.5 wt.					
35882879	5	29	theme	time	753:756	arg1	3.58 h					758:763	time 3.58 h	753:763	time 3.58 h with 11.68 mg of beads	753:786	The ideal conditions to achieve complete removal are 88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads composite with 2.5 wt.					
35882879	5	30	theme	cefotaxime	725:734	arg1	concentration					736:748	88.97 mg L-1 initial cefotaxime concentration	704:748	88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads	704:786	The ideal conditions to achieve complete removal are 88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads composite with 2.5 wt.					
35882879	9	31	theme	adsorption	1043:1052	arg1	process					1054:1060	adsorption process	1043:1060	adsorption process	1043:1060	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	11	32	theme	novel	1365:1369	arg1	beads					1385:1389	this novel nanocomposite beads	1360:1389	this novel nanocomposite beads has strong potential in water treatment for the antibiotic contaminant removal	1360:1468	This type of nanocomposites beads can be easily separated from water without leaving any residue, verifying this novel nanocomposite beads has strong potential in water treatment for the antibiotic contaminant removal.					
35882879	5	33	theme	complete	683:690	arg1	removal					692:698	complete removal	683:698	complete removal	683:698	The ideal conditions to achieve complete removal are 88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads composite with 2.5 wt.					
35882879	1	34	theme	vinyl	279:283	arg1	SPVA					294:297	SPVA	294:297	SPVA	294:297	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	1	34	theme	vinyl	279:283	arg1	alcohol					285:291	poly vinyl alcohol	274:291	poly vinyl alcohol (SPVA)	274:298	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	8	35	theme	SZrO2	1019:1023	arg1	SZrO2					1019:1023	SZrO2	1019:1023	SZrO2	1019:1023	% of SZrO2.					
35882879	8	35	theme	SZrO2	1019:1023	arg1	%					1014:1014	%	1014:1014	% of SZrO2.	1014:1024	% of SZrO2.					
35882879	7	36	dep	kinetics	846:853	arg1	model					855:859	model	855:859	model better illustrated the adsorption of cefotaxime on nanocomposite beads	855:930	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	11	37	theme	beads	1280:1284	arg1	type					1257:1260	This type	1252:1260	This type of nanocomposites beads	1252:1284	This type of nanocomposites beads can be easily separated from water without leaving any residue, verifying this novel nanocomposite beads has strong potential in water treatment for the antibiotic contaminant removal.					
35882879	9	38	theme	process	1054:1060	arg1	mechanism					1030:1038	The mechanism	1026:1038	The mechanism of adsorption process	1026:1060	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	1	39	theme	novel	201:205	arg1	beads					222:226	novel nanocomposites beads	201:226	novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2)	201:333	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	1	39	theme	novel	201:205	arg1	sulfonated					263:272	sulfonated	263:272	sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2)	263:333	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	2	40	theme	calcination	413:423	arg1	technique					425:433	solvent-free and easy calcination technique	391:433	solvent-free and easy calcination technique	391:433	SZrO2 was synthesized from solvent-free and easy calcination technique then embedded with 1-2.5 wt.					
35882879	0	41	theme	antibiotic	128:137	arg1	Cefotaxime					117:126	Cefotaxime	117:126	Cefotaxime antibiotic from aqueous solution	117:159	Development of hybrid green nanocomposite polymeric beads doped with nano sulfated zirconia for effective removal of Cefotaxime antibiotic from aqueous solution.					
35882879	7	42	theme	cefotaxime	898:907	arg1	adsorption					884:893	the adsorption	880:893	the adsorption of cefotaxime on nanocomposite beads	880:930	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	11	43	theme	antibiotic	1439:1448	arg1	removal					1462:1468	the antibiotic contaminant removal	1435:1468	the antibiotic contaminant removal	1435:1468	This type of nanocomposites beads can be easily separated from water without leaving any residue, verifying this novel nanocomposite beads has strong potential in water treatment for the antibiotic contaminant removal.					
35882879	1	44	theme	nanocomposites	207:220	arg1	beads					222:226	novel nanocomposites beads	201:226	novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2)	201:333	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	1	44	theme	nanocomposites	207:220	arg1	sulfonated					263:272	sulfonated	263:272	sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2)	263:333	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	0	45	theme	hybrid	15:20	arg1	green					22:26	hybrid green	15:26	hybrid green	15:26	Development of hybrid green nanocomposite polymeric beads doped with nano sulfated zirconia for effective removal of Cefotaxime antibiotic from aqueous solution.					
35882879	0	46	theme	Cefotaxime	117:126	arg1	removal					106:112	effective removal	96:112	effective removal of Cefotaxime antibiotic from aqueous solution	96:159	Development of hybrid green nanocomposite polymeric beads doped with nano sulfated zirconia for effective removal of Cefotaxime antibiotic from aqueous solution.					
35882879	5	47	with	composite	788:796	arg1	wt					807:808	2.5 wt	803:808	2.5 wt	803:808	The ideal conditions to achieve complete removal are 88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads composite with 2.5 wt.					
35882879	2	48	theme	solvent-free	391:402	arg1	technique					425:433	solvent-free and easy calcination technique	391:433	solvent-free and easy calcination technique	391:433	SZrO2 was synthesized from solvent-free and easy calcination technique then embedded with 1-2.5 wt.					
35882879	0	49	theme	polymeric	42:50	arg1	beads					52:56	polymeric beads	42:56	polymeric beads doped with nano sulfated zirconia for effective removal of Cefotaxime antibiotic from aqueous solution	42:159	Development of hybrid green nanocomposite polymeric beads doped with nano sulfated zirconia for effective removal of Cefotaxime antibiotic from aqueous solution.					
35882879	11	50	theme	water	1415:1419	arg1	treatment					1421:1429	water treatment	1415:1429	water treatment	1415:1429	This type of nanocomposites beads can be easily separated from water without leaving any residue, verifying this novel nanocomposite beads has strong potential in water treatment for the antibiotic contaminant removal.					
35882879	0	51	theme	aqueous	144:150	arg1	solution					152:159	aqueous solution	144:159	aqueous solution	144:159	Development of hybrid green nanocomposite polymeric beads doped with nano sulfated zirconia for effective removal of Cefotaxime antibiotic from aqueous solution.					
35882879	4	52	theme	antibiotic	628:637	arg1	adsorption					639:648	Cefotaxime antibiotic adsorption	617:648	Cefotaxime antibiotic adsorption	617:648	A batch adsorption experiment was carried out to investigate the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption.					
35882879	4	53	theme	time	609:612	arg1	effects					557:563	the effects	553:563	the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption	553:648	A batch adsorption experiment was carried out to investigate the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption.					
35882879	4	54	theme	adsorption	500:509	arg1	experiment					511:520	A batch adsorption experiment	492:520	A batch adsorption experiment	492:520	A batch adsorption experiment was carried out to investigate the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption.					
35882879	0	55	theme	green	22:26	arg1	Development					0:10	Development	0:10	Development of hybrid green	0:26	Development of hybrid green nanocomposite polymeric beads doped with nano sulfated zirconia for effective removal of Cefotaxime antibiotic from aqueous solution.					
35882879	5	56	theme	initial	717:723	arg1	concentration					736:748	88.97 mg L-1 initial cefotaxime concentration	704:748	88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads	704:786	The ideal conditions to achieve complete removal are 88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads composite with 2.5 wt.					
35882879	4	57	theme	batch	494:498	arg1	experiment					511:520	A batch adsorption experiment	492:520	A batch adsorption experiment	492:520	A batch adsorption experiment was carried out to investigate the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption.					
35882879	6	58	theme	SZrO2	815:819	arg1	%					810:810	%	810:810	% of SZrO2.	810:820	% of SZrO2.					
35882879	6	58	theme	SZrO2	815:819	arg1	SZrO2					815:819	SZrO2	815:819	SZrO2	815:819	% of SZrO2.					
35882879	11	59	dep	beads	1385:1389	arg1	has					1391:1393	has	1391:1393	has strong potential in water treatment for the antibiotic contaminant removal	1391:1468	This type of nanocomposites beads can be easily separated from water without leaving any residue, verifying this novel nanocomposite beads has strong potential in water treatment for the antibiotic contaminant removal.					
35882879	1	60	theme	nano	304:307	arg1	SZrO2					328:332	SZrO2	328:332	SZrO2	328:332	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	1	60	theme	nano	304:307	arg1	zirconia					318:325	nano sulfated zirconia	304:325	nano sulfated zirconia (SZrO2)	304:333	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	10	61	theme	excellent	1197:1205	arg1	reproducibility					1207:1221	excellent reproducibility	1197:1221	excellent reproducibility	1197:1221	The nanocomposites beads also exhibit excellent reproducibility after ten adsorption cycles.					
35882879	1	62	theme	sulfated	309:316	arg1	SZrO2					328:332	SZrO2	328:332	SZrO2	328:332	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	1	62	theme	sulfated	309:316	arg1	zirconia					318:325	nano sulfated zirconia	304:325	nano sulfated zirconia (SZrO2)	304:333	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	5	63	theme	ideal	655:659	arg1	conditions					661:670	The ideal conditions	651:670	The ideal conditions to achieve complete removal	651:698	The ideal conditions to achieve complete removal are 88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads composite with 2.5 wt.					
35882879	1	64	dep	composed	228:235	arg1	beads					222:226	novel nanocomposites beads	201:226	novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2)	201:333	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	1	64	dep	composed	228:235	arg1	sulfonated					263:272	sulfonated	263:272	sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2)	263:333	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	5	65	dep	conditions	661:670	arg1	achieve					675:681	achieve	675:681	to achieve complete removal	672:698	The ideal conditions to achieve complete removal are 88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads composite with 2.5 wt.					
35882879	1	66	theme	iota	240:243	arg1	IC					258:259	IC	258:259	IC	258:259	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	1	66	theme	iota	240:243	arg1	carrageenan					245:255	iota carrageenan	240:255	iota carrageenan (IC)	240:260	Adsorption efficiency of Cefotaxime by novel nanocomposites beads composed of iota carrageenan (IC), sulfonated poly vinyl alcohol (SPVA) and nano sulfated zirconia (SZrO2) was evaluated in this study.					
35882879	9	67	theme	functional	1118:1127	arg1	IC					1145:1146	IC	1145:1146	IC	1145:1146	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	67	theme	functional	1118:1127	arg1	SPVA					1139:1142	SPVA	1139:1142	SPVA	1139:1142	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	67	theme	functional	1118:1127	arg1	groups					1129:1134	the different functional groups	1104:1134	the different functional groups of SPVA, IC and SZrO2	1104:1156	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	67	theme	functional	1118:1127	arg1	SZrO2					1152:1156	SZrO2	1152:1156	SZrO2	1152:1156	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	11	68	from	potential	1402:1410	arg1	treatment					1421:1429	water treatment	1415:1429	water treatment	1415:1429	This type of nanocomposites beads can be easily separated from water without leaving any residue, verifying this novel nanocomposite beads has strong potential in water treatment for the antibiotic contaminant removal.					
35882879	9	69	theme	different	1108:1116	arg1	IC					1145:1146	IC	1145:1146	IC	1145:1146	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	69	theme	different	1108:1116	arg1	SPVA					1139:1142	SPVA	1139:1142	SPVA	1139:1142	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	69	theme	different	1108:1116	arg1	groups					1129:1134	the different functional groups	1104:1134	the different functional groups of SPVA, IC and SZrO2	1104:1156	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	9	69	theme	different	1108:1116	arg1	SZrO2					1152:1156	SZrO2	1152:1156	SZrO2	1152:1156	The mechanism of adsorption process depend mainly on the interactions between the different functional groups of SPVA, IC and SZrO2.					
35882879	11	70	theme	contaminant	1450:1460	arg1	removal					1462:1468	the antibiotic contaminant removal	1435:1468	the antibiotic contaminant removal	1435:1468	This type of nanocomposites beads can be easily separated from water without leaving any residue, verifying this novel nanocomposite beads has strong potential in water treatment for the antibiotic contaminant removal.					
35882879	11	71	theme	strong	1395:1400	arg1	potential					1402:1410	strong potential	1395:1410	strong potential in water treatment for the antibiotic contaminant removal	1395:1468	This type of nanocomposites beads can be easily separated from water without leaving any residue, verifying this novel nanocomposite beads has strong potential in water treatment for the antibiotic contaminant removal.					
35882879	0	72	theme	nano	69:72	arg1	zirconia					83:90	nano sulfated zirconia	69:90	nano sulfated zirconia	69:90	Development of hybrid green nanocomposite polymeric beads doped with nano sulfated zirconia for effective removal of Cefotaxime antibiotic from aqueous solution.					
35882879	11	73	theme	nanocomposites	1265:1278	arg1	beads					1280:1284	nanocomposites beads	1265:1284	nanocomposites beads	1265:1284	This type of nanocomposites beads can be easily separated from water without leaving any residue, verifying this novel nanocomposite beads has strong potential in water treatment for the antibiotic contaminant removal.					
35882879	2	74	with	embedded	440:447	arg1	wt					460:461	1-2.5 wt	454:461	1-2.5 wt	454:461	SZrO2 was synthesized from solvent-free and easy calcination technique then embedded with 1-2.5 wt.					
35882879	4	75	theme	beginning	580:588	arg1	concentration					590:602	beginning concentration	580:602	beginning concentration	580:602	A batch adsorption experiment was carried out to investigate the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption.					
35882879	2	76	theme	easy	408:411	arg1	technique					425:433	solvent-free and easy calcination technique	391:433	solvent-free and easy calcination technique	391:433	SZrO2 was synthesized from solvent-free and easy calcination technique then embedded with 1-2.5 wt.					
35882879	7	77	theme	maximum	941:947	arg1	capacity					960:967	the maximum adsorption capacity	937:967	the maximum adsorption capacity	937:967	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	7	77	theme	maximum	941:947	arg1	659 mg g-1					973:982	659 mg g-1	973:982	659 mg g-1	973:982	The pseudo second order kinetics model better illustrated the adsorption of cefotaxime on nanocomposite beads, and the maximum adsorption capacity are 659 mg g-1 for the composite with 2.5 wt.					
35882879	4	78	theme	pH	576:577	arg1	effects					557:563	the effects	553:563	the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption	553:648	A batch adsorption experiment was carried out to investigate the effects of dosage, pH, beginning concentration, and time on Cefotaxime antibiotic adsorption.					
35882879	10	79	theme	adsorption	1233:1242	arg1	cycles					1244:1249	ten adsorption cycles	1229:1249	ten adsorption cycles	1229:1249	The nanocomposites beads also exhibit excellent reproducibility after ten adsorption cycles.					
35882879	5	80	theme	L-1	713:715	arg1	concentration					736:748	88.97 mg L-1 initial cefotaxime concentration	704:748	88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads	704:786	The ideal conditions to achieve complete removal are 88.97 mg L-1 initial cefotaxime concentration at time 3.58 h with 11.68 mg of beads composite with 2.5 wt.					
36501037	4	0	theme	muscle	585:590	arg1	volume					592:597	muscle volume	585:597	muscle volume	585:597	IO supplementation increased muscle volume, exhaustive treadmill time, and glycogen storage in mice.					
36501037	0	1	theme	Inonotus	10:17	arg1	Supplementation					36:50	Inonotus obliquus Extract Supplementation	10:50	Inonotus obliquus Extract Supplementation	10:50	Effect of Inonotus obliquus Extract Supplementation on Endurance Exercise and Energy-Consuming Processes through Lipid Transport in Mice.					
36501037	6	2	theme	increased	904:912	arg1	signaling					919:927	significantly increased PPAR signaling	890:927	significantly increased PPAR signaling	890:927	RNA sequencing revealed significantly increased PPAR signaling; phenylalanine, ascorbate, aldarate, and cholesterol metabolism; chemical carcinogenesis; and ergosterol biosynthesis in the IO group compared with the vehicle group.					
36501037	0	3	theme	Energy-Consuming	78:93	arg1	Processes					95:103	Energy-Consuming Processes	78:103	Energy-Consuming Processes	78:103	Effect of Inonotus obliquus Extract Supplementation on Endurance Exercise and Energy-Consuming Processes through Lipid Transport in Mice.					
36501037	3	4	theme	daily	474:478	arg1	IO					466:467	IO	466:467	IO once daily for 6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×)	466:553	Male Institute of Cancer Research mice were divided into four groups (n = 8 per group) and orally administered IO once daily for 6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×).					
36501037	3	5	from	0	492:492	arg1	wk					486:487	6 wk	484:487	6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×)	484:553	Male Institute of Cancer Research mice were divided into four groups (n = 8 per group) and orally administered IO once daily for 6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×).					
36501037	0	6	theme	Lipid	113:117	arg1	Transport					119:127	Lipid Transport	113:127	Lipid Transport in Mice	113:135	Effect of Inonotus obliquus Extract Supplementation on Endurance Exercise and Energy-Consuming Processes through Lipid Transport in Mice.					
36501037	0	7	from	Effect	0:5	arg1	Exercise					65:72	Endurance Exercise	55:72	Endurance Exercise	55:72	Effect of Inonotus obliquus Extract Supplementation on Endurance Exercise and Energy-Consuming Processes through Lipid Transport in Mice.					
36501037	0	7	from	Effect	0:5	arg1	Processes					95:103	Energy-Consuming Processes	78:103	Energy-Consuming Processes	78:103	Effect of Inonotus obliquus Extract Supplementation on Endurance Exercise and Energy-Consuming Processes through Lipid Transport in Mice.					
36501037	2	8	from	changes	299:305	arg1	expenditure					317:327	energy expenditure	310:327	energy expenditure	310:327	This study investigated the effect of IO supplementation on body composition in relation to changes in energy expenditure and exercise performance.					
36501037	2	8	from	changes	299:305	arg1	performance					342:352	exercise performance	333:352	exercise performance	333:352	This study investigated the effect of IO supplementation on body composition in relation to changes in energy expenditure and exercise performance.					
36501037	7	9	theme	lipid	1161:1165	arg1	transport					1167:1175	lipid transport	1161:1175	lipid transport	1161:1175	Thus, IO supplements as nutraceuticals have a positive effect on lipid transport and exercise performance.					
36501037	6	10	theme	vehicle	1081:1087	arg1	group					1089:1093	the vehicle group	1077:1093	the vehicle group	1077:1093	RNA sequencing revealed significantly increased PPAR signaling; phenylalanine, ascorbate, aldarate, and cholesterol metabolism; chemical carcinogenesis; and ergosterol biosynthesis in the IO group compared with the vehicle group.					
36501037	5	11	theme	peroxisome	807:816	arg1	PPAR					851:854	PPAR	851:854	PPAR	851:854	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	5	11	theme	peroxisome	807:816	arg1	receptor					841:848	the peroxisome proliferator-activated receptor	803:848	the peroxisome proliferator-activated receptor (PPAR) pathway	803:863	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	4	12	theme	treadmill	611:619	arg1	time					621:624	exhaustive treadmill time	600:624	exhaustive treadmill time	600:624	IO supplementation increased muscle volume, exhaustive treadmill time, and glycogen storage in mice.					
36501037	4	13	theme	exhaustive	600:609	arg1	time					621:624	exhaustive treadmill time	600:624	exhaustive treadmill time	600:624	IO supplementation increased muscle volume, exhaustive treadmill time, and glycogen storage in mice.					
36501037	6	14	theme	chemical	994:1001	arg1	carcinogenesis					1003:1016	chemical carcinogenesis	994:1016	chemical carcinogenesis	994:1016	RNA sequencing revealed significantly increased PPAR signaling; phenylalanine, ascorbate, aldarate, and cholesterol metabolism; chemical carcinogenesis; and ergosterol biosynthesis in the IO group compared with the vehicle group.					
36501037	4	15	from	storage	640:646	arg1	mice					651:654	mice	651:654	mice	651:654	IO supplementation increased muscle volume, exhaustive treadmill time, and glycogen storage in mice.					
36501037	2	16	theme	body	267:270	arg1	composition					272:282	body composition	267:282	body composition in relation to changes in energy expenditure and exercise performance	267:352	This study investigated the effect of IO supplementation on body composition in relation to changes in energy expenditure and exercise performance.					
36501037	5	17	theme	supplementation	726:740	arg1	group					742:746	the IO supplementation group	719:746	the IO supplementation group	719:746	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	3	18	from	mg/kg	541:545	arg1	wk					486:487	6 wk	484:487	6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×)	484:553	Male Institute of Cancer Research mice were divided into four groups (n = 8 per group) and orally administered IO once daily for 6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×).					
36501037	5	19	theme	Serum	657:661	arg1	acid					674:677	Serum free fatty acid	657:677	Serum free fatty acid levels after acute exercise	657:705	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	3	20	from	IO-2×	524:528	arg1	wk					486:487	6 wk	484:487	6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×)	484:553	Male Institute of Cancer Research mice were divided into four groups (n = 8 per group) and orally administered IO once daily for 6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×).					
36501037	3	21	theme	=	427:427	arg1	n					425:425	n = 8	425:429	n = 8	425:429	Male Institute of Cancer Research mice were divided into four groups (n = 8 per group) and orally administered IO once daily for 6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×).					
36501037	3	22	theme	Research	380:387	arg1	mice					389:392	Cancer Research mice	373:392	Cancer Research mice	373:392	Male Institute of Cancer Research mice were divided into four groups (n = 8 per group) and orally administered IO once daily for 6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×).					
36501037	8	23	from	supplementation	1239:1253	arg1	addition					1206:1213	addition	1206:1213	addition	1206:1213	In addition, this study was only IO supplementation without training-related procedures.					
36501037	5	24	theme	proliferator-activated	818:839	arg1	PPAR					851:854	PPAR	851:854	PPAR	851:854	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	5	24	theme	proliferator-activated	818:839	arg1	receptor					841:848	the peroxisome proliferator-activated receptor	803:848	the peroxisome proliferator-activated receptor (PPAR) pathway	803:863	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	1	25	theme	functional	172:181	arg1	food					183:186	functional food	172:186	functional food	172:186	Inonotus obliquus (IO) is used as functional food to treat diabetes.					
36501037	1	25	theme	functional	172:181	arg1	obliquus					147:154	Inonotus obliquus	138:154	Inonotus obliquus (IO)	138:159	Inonotus obliquus (IO) is used as functional food to treat diabetes.					
36501037	3	26	theme	mice	389:392	arg1	Institute					360:368	Male Institute	355:368	Male Institute of Cancer Research mice	355:392	Male Institute of Cancer Research mice were divided into four groups (n = 8 per group) and orally administered IO once daily for 6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×).					
36501037	6	27	theme	RNA	866:868	arg1	sequencing					870:879	RNA sequencing	866:879	RNA sequencing	866:879	RNA sequencing revealed significantly increased PPAR signaling; phenylalanine, ascorbate, aldarate, and cholesterol metabolism; chemical carcinogenesis; and ergosterol biosynthesis in the IO group compared with the vehicle group.					
36501037	5	28	theme	receptor	841:848	arg1	pathway					857:863	the peroxisome proliferator-activated receptor (PPAR) pathway	803:863	the peroxisome proliferator-activated receptor (PPAR) pathway	803:863	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	0	29	theme	obliquus	19:26	arg1	Supplementation					36:50	Inonotus obliquus Extract Supplementation	10:50	Inonotus obliquus Extract Supplementation	10:50	Effect of Inonotus obliquus Extract Supplementation on Endurance Exercise and Energy-Consuming Processes through Lipid Transport in Mice.					
36501037	6	30	theme	cholesterol	970:980	arg1	metabolism					982:991	cholesterol metabolism	970:991	cholesterol metabolism	970:991	RNA sequencing revealed significantly increased PPAR signaling; phenylalanine, ascorbate, aldarate, and cholesterol metabolism; chemical carcinogenesis; and ergosterol biosynthesis in the IO group compared with the vehicle group.					
36501037	4	31	from	time	621:624	arg1	mice					651:654	mice	651:654	mice	651:654	IO supplementation increased muscle volume, exhaustive treadmill time, and glycogen storage in mice.					
36501037	4	32	theme	glycogen	631:638	arg1	storage					640:646	glycogen storage	631:646	glycogen storage in mice	631:654	IO supplementation increased muscle volume, exhaustive treadmill time, and glycogen storage in mice.					
36501037	2	33	from	effect	235:240	arg1	composition					272:282	body composition	267:282	body composition in relation to changes in energy expenditure and exercise performance	267:352	This study investigated the effect of IO supplementation on body composition in relation to changes in energy expenditure and exercise performance.					
36501037	5	34	from	changes	765:771	arg1	expenditure					783:793	energy expenditure	776:793	energy expenditure	776:793	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	3	35	theme	Cancer	373:378	arg1	mice					389:392	Cancer Research mice	373:392	Cancer Research mice	373:392	Male Institute of Cancer Research mice were divided into four groups (n = 8 per group) and orally administered IO once daily for 6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×).					
36501037	5	36	theme	energy	776:781	arg1	expenditure					783:793	energy expenditure	776:793	energy expenditure	776:793	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	0	37	theme	Supplementation	36:50	arg1	Effect					0:5	Effect	0:5	Effect of Inonotus obliquus Extract Supplementation on Endurance Exercise and Energy-Consuming Processes through Lipid Transport in Mice.	0:136	Effect of Inonotus obliquus Extract Supplementation on Endurance Exercise and Energy-Consuming Processes through Lipid Transport in Mice.					
36501037	3	38	dep	groups	417:422	arg1	n					425:425	n = 8	425:429	n = 8	425:429	Male Institute of Cancer Research mice were divided into four groups (n = 8 per group) and orally administered IO once daily for 6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×).					
36501037	4	39	from	volume	592:597	arg1	mice					651:654	mice	651:654	mice	651:654	IO supplementation increased muscle volume, exhaustive treadmill time, and glycogen storage in mice.					
36501037	0	40	theme	Extract	28:34	arg1	Supplementation					36:50	Inonotus obliquus Extract Supplementation	10:50	Inonotus obliquus Extract Supplementation	10:50	Effect of Inonotus obliquus Extract Supplementation on Endurance Exercise and Energy-Consuming Processes through Lipid Transport in Mice.					
36501037	5	41	theme	free	663:666	arg1	acid					674:677	Serum free fatty acid	657:677	Serum free fatty acid levels after acute exercise	657:705	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	5	42	theme	acute	692:696	arg1	exercise					698:705	acute exercise	692:705	acute exercise	692:705	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	5	43	theme	IO	723:724	arg1	group					742:746	the IO supplementation group	719:746	the IO supplementation group	719:746	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	0	44	theme	Endurance	55:63	arg1	Exercise					65:72	Endurance Exercise	55:72	Endurance Exercise	55:72	Effect of Inonotus obliquus Extract Supplementation on Endurance Exercise and Energy-Consuming Processes through Lipid Transport in Mice.					
36501037	3	45	theme	Male	355:358	arg1	Institute					360:368	Male Institute	355:368	Male Institute of Cancer Research mice	355:392	Male Institute of Cancer Research mice were divided into four groups (n = 8 per group) and orally administered IO once daily for 6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×).					
36501037	6	46	theme	ergosterol	1023:1032	arg1	biosynthesis					1034:1045	ergosterol biosynthesis	1023:1045	ergosterol biosynthesis	1023:1045	RNA sequencing revealed significantly increased PPAR signaling; phenylalanine, ascorbate, aldarate, and cholesterol metabolism; chemical carcinogenesis; and ergosterol biosynthesis in the IO group compared with the vehicle group.					
36501037	2	47	theme	exercise	333:340	arg1	performance					342:352	exercise performance	333:352	exercise performance	333:352	This study investigated the effect of IO supplementation on body composition in relation to changes in energy expenditure and exercise performance.					
36501037	5	48	theme	acid	674:677	arg1	levels					679:684	Serum free fatty acid levels	657:684	Serum free fatty acid levels after acute exercise	657:705	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	7	49	theme	positive	1142:1149	arg1	effect					1151:1156	a positive effect	1140:1156	a positive effect	1140:1156	Thus, IO supplements as nutraceuticals have a positive effect on lipid transport and exercise performance.					
36501037	7	50	theme	exercise	1181:1188	arg1	performance					1190:1200	exercise performance	1181:1200	exercise performance	1181:1200	Thus, IO supplements as nutraceuticals have a positive effect on lipid transport and exercise performance.					
36501037	0	51	from	Transport	119:127	arg1	Mice					132:135	Mice	132:135	Mice	132:135	Effect of Inonotus obliquus Extract Supplementation on Endurance Exercise and Energy-Consuming Processes through Lipid Transport in Mice.					
36501037	2	52	theme	supplementation	248:262	arg1	effect					235:240	the effect	231:240	the effect of IO supplementation on body composition in relation to changes in energy expenditure and exercise performance	231:352	This study investigated the effect of IO supplementation on body composition in relation to changes in energy expenditure and exercise performance.					
36501037	1	53	theme	Inonotus	138:145	arg1	IO					157:158	IO	157:158	IO	157:158	Inonotus obliquus (IO) is used as functional food to treat diabetes.					
36501037	1	53	theme	Inonotus	138:145	arg1	obliquus					147:154	Inonotus obliquus	138:154	Inonotus obliquus (IO)	138:159	Inonotus obliquus (IO) is used as functional food to treat diabetes.					
36501037	1	53	theme	Inonotus	138:145	arg1	food					183:186	functional food	172:186	functional food	172:186	Inonotus obliquus (IO) is used as functional food to treat diabetes.					
36501037	6	54	theme	IO	1054:1055	arg1	group					1057:1061	the IO group	1050:1061	the IO group	1050:1061	RNA sequencing revealed significantly increased PPAR signaling; phenylalanine, ascorbate, aldarate, and cholesterol metabolism; chemical carcinogenesis; and ergosterol biosynthesis in the IO group compared with the vehicle group.					
36501037	2	55	theme	IO	245:246	arg1	supplementation					248:262	IO supplementation	245:262	IO supplementation	245:262	This study investigated the effect of IO supplementation on body composition in relation to changes in energy expenditure and exercise performance.					
36501037	2	56	theme	energy	310:315	arg1	expenditure					317:327	energy expenditure	310:327	energy expenditure	310:327	This study investigated the effect of IO supplementation on body composition in relation to changes in energy expenditure and exercise performance.					
36501037	8	57	theme	training-related	1263:1278	arg1	procedures					1280:1289	training-related procedures	1263:1289	training-related procedures	1263:1289	In addition, this study was only IO supplementation without training-related procedures.					
36501037	3	58	from	IO-1×	510:514	arg1	wk					486:487	6 wk	484:487	6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×)	484:553	Male Institute of Cancer Research mice were divided into four groups (n = 8 per group) and orally administered IO once daily for 6 wk at 0 (vehicle), 824 (IO-1×), 1648 (IO-2×), and 2472 mg/kg (IO-3×).					
36501037	2	59	from	composition	272:282	arg1	relation					287:294	relation	287:294	relation to changes in energy expenditure and exercise performance	287:352	This study investigated the effect of IO supplementation on body composition in relation to changes in energy expenditure and exercise performance.					
36501037	5	60	theme	fatty	668:672	arg1	acid					674:677	Serum free fatty acid	657:677	Serum free fatty acid levels after acute exercise	657:705	Serum free fatty acid levels after acute exercise improved in the IO supplementation group, which exhibited changes in energy expenditure through the peroxisome proliferator-activated receptor (PPAR) pathway.					
36501037	1	61	used	used	164:167	arg2	food					183:186	functional food	172:186	functional food	172:186	Inonotus obliquus (IO) is used as functional food to treat diabetes.					
36501037	1	61	used	used	164:167	arg2	IO					157:158	IO	157:158	IO	157:158	Inonotus obliquus (IO) is used as functional food to treat diabetes.					
36501037	1	61	used	used	164:167	arg2	obliquus					147:154	Inonotus obliquus	138:154	Inonotus obliquus (IO)	138:159	Inonotus obliquus (IO) is used as functional food to treat diabetes.					
36501037	7	62	contain	have	1135:1138	arg1	nutraceuticals					1120:1133	nutraceuticals	1120:1133	nutraceuticals	1120:1133	Thus, IO supplements as nutraceuticals have a positive effect on lipid transport and exercise performance.					
36501037	7	62	contain	have	1135:1138	arg2	effect					1151:1156	a positive effect	1140:1156	a positive effect	1140:1156	Thus, IO supplements as nutraceuticals have a positive effect on lipid transport and exercise performance.					
36501037	4	63	theme	IO	556:557	arg1	supplementation					559:573	IO supplementation	556:573	IO supplementation	556:573	IO supplementation increased muscle volume, exhaustive treadmill time, and glycogen storage in mice.					
36501037	6	64	theme	PPAR	914:917	arg1	signaling					919:927	significantly increased PPAR signaling	890:927	significantly increased PPAR signaling	890:927	RNA sequencing revealed significantly increased PPAR signaling; phenylalanine, ascorbate, aldarate, and cholesterol metabolism; chemical carcinogenesis; and ergosterol biosynthesis in the IO group compared with the vehicle group.					
36296679	2	0	theme	abnormal	322:329	arg1	patterns					345:352	abnormal glycosylation patterns	322:352	abnormal glycosylation patterns	322:352	In GBMs, abnormal glycosylation patterns are associated with malignancy, which allows for the use of lectins as tools for recognition and therapy.					
36296679	7	1	dep	in	1122:1123	arg1	silico					1125:1130	silico	1125:1130	silico	1125:1130	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	0	2	theme	Lectin	76:81	arg1	Characterization					26:41	Characterization	26:41	Characterization	26:41	Structural Prediction and Characterization of Canavalia grandiflora (ConGF) Lectin Complexed with MMP1: Unveiling the Antiglioma Potential of Legume Lectins.					
36296679	0	2	theme	Lectin	76:81	arg1	Prediction					11:20	Structural Prediction	0:20	Structural Prediction	0:20	Structural Prediction and Characterization of Canavalia grandiflora (ConGF) Lectin Complexed with MMP1: Unveiling the Antiglioma Potential of Legume Lectins.					
36296679	5	3	theme	morphological	845:857	arg1	changes					859:865	morphological changes	845:865	morphological changes	845:865	The treatment of C6 cells with ConGF impaired the mitochondrial transmembrane potential, reduced cell viability, and induced morphological changes.					
36296679	1	4	theme	poor	228:231	arg1	prognosis					233:241	a poor prognosis	226:241	a poor prognosis	226:241	A glioblastoma (GBM) is a highly malignant primary brain tumor with a poor prognosis because of its invasiveness and high resistance to current therapies.					
36296679	3	5	theme	glycan	505:510	arg1	structures					512:521	glycan structures	505:521	glycan structures found on the malignant cell surface	505:557	More specifically, lectins can interact with glycan structures found on the malignant cell surface.					
36296679	7	6	theme	action	1048:1053	arg1	mechanism					1035:1043	The mechanism	1031:1043	The mechanism of action	1031:1053	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	4	7	theme	C6	710:711	arg1	cells					713:717	C6 cells	710:717	C6 cells	710:717	In this context, the present work aimed to investigate the antiglioma potential of ConGF, a lectin purified from Canavalia grandiflora seeds, against C6 cells.					
36296679	7	8	theme	in	1122:1123	arg1	studies					1132:1138	in silico studies	1122:1138	in silico studies	1122:1138	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	5	9	theme	transmembrane	784:796	arg1	potential					798:806	the mitochondrial transmembrane potential	766:806	the mitochondrial transmembrane potential	766:806	The treatment of C6 cells with ConGF impaired the mitochondrial transmembrane potential, reduced cell viability, and induced morphological changes.					
36296679	5	10	with	treatment	724:732	arg1	ConGF					751:755	ConGF	751:755	ConGF	751:755	The treatment of C6 cells with ConGF impaired the mitochondrial transmembrane potential, reduced cell viability, and induced morphological changes.					
36296679	6	11	theme	prominent	989:997	arg1	PI					1017:1018	PI	1017:1018	PI	1017:1018	ConGF also induced massive autophagy, as evaluated by acridine orange (AO) staining and LC3AB-II expression, but without prominent propidium iodide (PI) labeling.					
36296679	6	11	theme	prominent	989:997	arg1	iodide					1009:1014	prominent propidium iodide	989:1014	prominent propidium iodide (PI) labeling	989:1028	ConGF also induced massive autophagy, as evaluated by acridine orange (AO) staining and LC3AB-II expression, but without prominent propidium iodide (PI) labeling.					
36296679	5	12	theme	cells	740:744	arg1	treatment					724:732	The treatment	720:732	The treatment of C6 cells with ConGF	720:755	The treatment of C6 cells with ConGF impaired the mitochondrial transmembrane potential, reduced cell viability, and induced morphological changes.					
36296679	7	13	theme	carbohydrate-binding	1078:1097	arg1	capacity					1099:1106	the carbohydrate-binding capacity	1074:1106	the carbohydrate-binding capacity of ConGF	1074:1115	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	5	14	theme	cell	817:820	arg1	viability					822:830	cell viability	817:830	cell viability	817:830	The treatment of C6 cells with ConGF impaired the mitochondrial transmembrane potential, reduced cell viability, and induced morphological changes.					
36296679	2	15	theme	lectins	414:420	arg1	use					407:409	the use	403:409	the use of lectins as tools for recognition and therapy	403:457	In GBMs, abnormal glycosylation patterns are associated with malignancy, which allows for the use of lectins as tools for recognition and therapy.					
36296679	3	16	located	found	523:527	arg2	structures					512:521	glycan structures	505:521	glycan structures found on the malignant cell surface	505:557	More specifically, lectins can interact with glycan structures found on the malignant cell surface.					
36296679	3	16	located	found	523:527	arg1	surface					551:557	the malignant cell surface	532:557	the malignant cell surface	532:557	More specifically, lectins can interact with glycan structures found on the malignant cell surface.					
36296679	0	17	theme	Structural	0:9	arg1	Prediction					11:20	Structural Prediction	0:20	Structural Prediction	0:20	Structural Prediction and Characterization of Canavalia grandiflora (ConGF) Lectin Complexed with MMP1: Unveiling the Antiglioma Potential of Legume Lectins.					
36296679	6	18	theme	orange	931:936	arg1	staining					943:950	acridine orange (AO) staining	922:950	acridine orange (AO) staining	922:950	ConGF also induced massive autophagy, as evaluated by acridine orange (AO) staining and LC3AB-II expression, but without prominent propidium iodide (PI) labeling.					
36296679	6	19	theme	massive	887:893	arg1	autophagy					895:903	massive autophagy	887:903	massive autophagy	887:903	ConGF also induced massive autophagy, as evaluated by acridine orange (AO) staining and LC3AB-II expression, but without prominent propidium iodide (PI) labeling.					
36296679	3	20	theme	malignant	536:544	arg1	surface					551:557	the malignant cell surface	532:557	the malignant cell surface	532:557	More specifically, lectins can interact with glycan structures found on the malignant cell surface.					
36296679	4	21	theme	antiglioma	619:628	arg1	potential					630:638	the antiglioma potential	615:638	the antiglioma potential of ConGF, a lectin purified from Canavalia grandiflora seeds, against C6 cells	615:717	In this context, the present work aimed to investigate the antiglioma potential of ConGF, a lectin purified from Canavalia grandiflora seeds, against C6 cells.					
36296679	6	22	theme	acridine	922:929	arg1	AO					939:940	AO	939:940	AO	939:940	ConGF also induced massive autophagy, as evaluated by acridine orange (AO) staining and LC3AB-II expression, but without prominent propidium iodide (PI) labeling.					
36296679	6	22	theme	acridine	922:929	arg1	orange					931:936	acridine orange	922:936	acridine orange (AO) staining	922:950	ConGF also induced massive autophagy, as evaluated by acridine orange (AO) staining and LC3AB-II expression, but without prominent propidium iodide (PI) labeling.					
36296679	0	23	theme	Antiglioma	118:127	arg1	Potential					129:137	the Antiglioma Potential	114:137	the Antiglioma Potential of Legume Lectins	114:155	Structural Prediction and Characterization of Canavalia grandiflora (ConGF) Lectin Complexed with MMP1: Unveiling the Antiglioma Potential of Legume Lectins.					
36296679	7	24	theme	observed	1312:1319	arg1	effects					1321:1327	the observed effects	1308:1327	the observed effects	1308:1327	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	5	25	theme	mitochondrial	770:782	arg1	potential					798:806	the mitochondrial transmembrane potential	766:806	the mitochondrial transmembrane potential	766:806	The treatment of C6 cells with ConGF impaired the mitochondrial transmembrane potential, reduced cell viability, and induced morphological changes.					
36296679	4	26	theme	grandiflora	683:693	arg1	seeds					695:699	Canavalia grandiflora seeds	673:699	Canavalia grandiflora seeds	673:699	In this context, the present work aimed to investigate the antiglioma potential of ConGF, a lectin purified from Canavalia grandiflora seeds, against C6 cells.					
36296679	4	27	theme	present	581:587	arg1	work					589:592	the present work	577:592	the present work	577:592	In this context, the present work aimed to investigate the antiglioma potential of ConGF, a lectin purified from Canavalia grandiflora seeds, against C6 cells.					
36296679	0	28	theme	Canavalia	46:54	arg1	Lectin					76:81	Canavalia grandiflora (ConGF) Lectin	46:81	Canavalia grandiflora (ConGF) Lectin Complexed with MMP1	46:101	Structural Prediction and Characterization of Canavalia grandiflora (ConGF) Lectin Complexed with MMP1: Unveiling the Antiglioma Potential of Legume Lectins.					
36296679	6	29	theme	iodide	1009:1014	arg1	labeling					1021:1028	prominent propidium iodide (PI) labeling	989:1028	prominent propidium iodide (PI) labeling	989:1028	ConGF also induced massive autophagy, as evaluated by acridine orange (AO) staining and LC3AB-II expression, but without prominent propidium iodide (PI) labeling.					
36296679	7	30	theme	prominent	1246:1254	arg1	metalloproteinase					1216:1232	matrix metalloproteinase 1	1209:1234	matrix metalloproteinase 1 (MMP1)	1209:1241	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	7	30	theme	prominent	1246:1254	arg1	protein					1256:1262	a prominent protein	1244:1262	a prominent protein found in malignant cells	1244:1287	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	1	31	theme	high	275:278	arg1	resistance					280:289	high resistance	275:289	high resistance to current therapies	275:310	A glioblastoma (GBM) is a highly malignant primary brain tumor with a poor prognosis because of its invasiveness and high resistance to current therapies.					
36296679	1	32	theme	malignant	191:199	arg1	tumor					215:219	a highly malignant primary brain tumor	182:219	a highly malignant primary brain tumor with a poor prognosis	182:241	A glioblastoma (GBM) is a highly malignant primary brain tumor with a poor prognosis because of its invasiveness and high resistance to current therapies.					
36296679	1	32	theme	malignant	191:199	arg1	glioblastoma					160:171	A glioblastoma	158:171	A glioblastoma (GBM)	158:177	A glioblastoma (GBM) is a highly malignant primary brain tumor with a poor prognosis because of its invasiveness and high resistance to current therapies.					
36296679	5	33	theme	C6	737:738	arg1	cells					740:744	C6 cells	737:744	C6 cells	737:744	The treatment of C6 cells with ConGF impaired the mitochondrial transmembrane potential, reduced cell viability, and induced morphological changes.					
36296679	0	34	theme	Lectins	149:155	arg1	Potential					129:137	the Antiglioma Potential	114:137	the Antiglioma Potential of Legume Lectins	114:155	Structural Prediction and Characterization of Canavalia grandiflora (ConGF) Lectin Complexed with MMP1: Unveiling the Antiglioma Potential of Legume Lectins.					
36296679	3	35	theme	cell	546:549	arg1	surface					551:557	the malignant cell surface	532:557	the malignant cell surface	532:557	More specifically, lectins can interact with glycan structures found on the malignant cell surface.					
36296679	7	36	theme	matrix	1209:1214	arg1	MMP1					1237:1240	MMP1	1237:1240	MMP1	1237:1240	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	7	36	theme	matrix	1209:1214	arg1	protein					1256:1262	a prominent protein	1244:1262	a prominent protein found in malignant cells	1244:1287	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	7	36	theme	matrix	1209:1214	arg1	metalloproteinase					1216:1232	matrix metalloproteinase 1	1209:1234	matrix metalloproteinase 1 (MMP1)	1209:1241	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	0	37	theme	grandiflora	56:66	arg1	Lectin					76:81	Canavalia grandiflora (ConGF) Lectin	46:81	Canavalia grandiflora (ConGF) Lectin Complexed with MMP1	46:101	Structural Prediction and Characterization of Canavalia grandiflora (ConGF) Lectin Complexed with MMP1: Unveiling the Antiglioma Potential of Legume Lectins.					
36296679	0	38	theme	Legume	142:147	arg1	Lectins					149:155	Legume Lectins	142:155	Legume Lectins	142:155	Structural Prediction and Characterization of Canavalia grandiflora (ConGF) Lectin Complexed with MMP1: Unveiling the Antiglioma Potential of Legume Lectins.					
36296679	7	39	theme	metalloproteinase	1216:1232	arg1	structures					1195:1204	the glycan structures	1184:1204	the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells	1184:1287	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	1	40	theme	current	294:300	arg1	therapies					302:310	current therapies	294:310	current therapies	294:310	A glioblastoma (GBM) is a highly malignant primary brain tumor with a poor prognosis because of its invasiveness and high resistance to current therapies.					
36296679	7	41	theme	malignant	1273:1281	arg1	cells					1283:1287	malignant cells	1273:1287	malignant cells	1273:1287	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	4	42	theme	Canavalia	673:681	arg1	seeds					695:699	Canavalia grandiflora seeds	673:699	Canavalia grandiflora seeds	673:699	In this context, the present work aimed to investigate the antiglioma potential of ConGF, a lectin purified from Canavalia grandiflora seeds, against C6 cells.					
36296679	1	43	theme	primary	201:207	arg1	tumor					215:219	a highly malignant primary brain tumor	182:219	a highly malignant primary brain tumor with a poor prognosis	182:241	A glioblastoma (GBM) is a highly malignant primary brain tumor with a poor prognosis because of its invasiveness and high resistance to current therapies.					
36296679	1	43	theme	primary	201:207	arg1	glioblastoma					160:171	A glioblastoma	158:171	A glioblastoma (GBM)	158:177	A glioblastoma (GBM) is a highly malignant primary brain tumor with a poor prognosis because of its invasiveness and high resistance to current therapies.					
36296679	1	44	with	tumor	215:219	arg1	prognosis					233:241	a poor prognosis	226:241	a poor prognosis	226:241	A glioblastoma (GBM) is a highly malignant primary brain tumor with a poor prognosis because of its invasiveness and high resistance to current therapies.					
36296679	7	45	theme	glycan	1188:1193	arg1	structures					1195:1204	the glycan structures	1184:1204	the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells	1184:1287	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	1	46	theme	brain	209:213	arg1	tumor					215:219	a highly malignant primary brain tumor	182:219	a highly malignant primary brain tumor with a poor prognosis	182:241	A glioblastoma (GBM) is a highly malignant primary brain tumor with a poor prognosis because of its invasiveness and high resistance to current therapies.					
36296679	1	46	theme	brain	209:213	arg1	glioblastoma					160:171	A glioblastoma	158:171	A glioblastoma (GBM)	158:177	A glioblastoma (GBM) is a highly malignant primary brain tumor with a poor prognosis because of its invasiveness and high resistance to current therapies.					
36296679	6	47	theme	propidium	999:1007	arg1	PI					1017:1018	PI	1017:1018	PI	1017:1018	ConGF also induced massive autophagy, as evaluated by acridine orange (AO) staining and LC3AB-II expression, but without prominent propidium iodide (PI) labeling.					
36296679	6	47	theme	propidium	999:1007	arg1	iodide					1009:1014	prominent propidium iodide	989:1014	prominent propidium iodide (PI) labeling	989:1028	ConGF also induced massive autophagy, as evaluated by acridine orange (AO) staining and LC3AB-II expression, but without prominent propidium iodide (PI) labeling.					
36296679	4	48	theme	ConGF	643:647	arg1	potential					630:638	the antiglioma potential	615:638	the antiglioma potential of ConGF, a lectin purified from Canavalia grandiflora seeds, against C6 cells	615:717	In this context, the present work aimed to investigate the antiglioma potential of ConGF, a lectin purified from Canavalia grandiflora seeds, against C6 cells.					
36296679	6	49	theme	LC3AB-II	956:963	arg1	expression					965:974	LC3AB-II expression	956:974	LC3AB-II expression	956:974	ConGF also induced massive autophagy, as evaluated by acridine orange (AO) staining and LC3AB-II expression, but without prominent propidium iodide (PI) labeling.					
36296679	0	50	theme	ConGF	69:73	arg1	Lectin					76:81	Canavalia grandiflora (ConGF) Lectin	46:81	Canavalia grandiflora (ConGF) Lectin Complexed with MMP1	46:101	Structural Prediction and Characterization of Canavalia grandiflora (ConGF) Lectin Complexed with MMP1: Unveiling the Antiglioma Potential of Legume Lectins.					
36296679	7	51	theme	ConGF	1111:1115	arg1	capacity					1099:1106	the carbohydrate-binding capacity	1074:1106	the carbohydrate-binding capacity of ConGF	1074:1115	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	2	52	theme	glycosylation	331:343	arg1	patterns					345:352	abnormal glycosylation patterns	322:352	abnormal glycosylation patterns	322:352	In GBMs, abnormal glycosylation patterns are associated with malignancy, which allows for the use of lectins as tools for recognition and therapy.					
36296679	7	53	located	found	1264:1268	arg2	protein					1256:1262	a prominent protein	1244:1262	a prominent protein found in malignant cells	1244:1287	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	7	53	located	found	1264:1268	arg2	metalloproteinase					1216:1232	matrix metalloproteinase 1	1209:1234	matrix metalloproteinase 1 (MMP1)	1209:1241	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36296679	7	53	located	found	1264:1268	arg1	cells					1283:1287	malignant cells	1273:1287	malignant cells	1273:1287	The mechanism of action appears to involve the carbohydrate-binding capacity of ConGF, and in silico studies suggested that the lectin can interact with the glycan structures of matrix metalloproteinase 1 (MMP1), a prominent protein found in malignant cells, likely explaining the observed effects.					
36588500	5	0	theme	MAIN	613:616	arg1	RESULTS					618:624	MAIN RESULTS	613:624	MAIN RESULTS	613:624	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	7	1	dep	Xa	1196:1197	arg1	inhibitors					1199:1208	inhibitors	1199:1208	inhibitors	1199:1208	CONCLUSIONS Our results suggest that it may be preferable to continue patients in AKI on factor Xa inhibitors versus transitioning to UFH due to the lower risk of bleeding events.					
36588500	6	2	theme	decreased	897:905	arg1	risk					907:910	a significantly decreased risk	881:910	a significantly decreased risk of CRNMB events in the factor Xa inhibitor group	881:959	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	1	3	theme	heparin	262:268	arg1	doses					207:211	therapeutic doses	195:211	therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH)	195:274	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	7	4	theme	lower	1249:1253	arg1	risk					1255:1258	the lower risk	1245:1258	the lower risk of bleeding events	1245:1277	CONCLUSIONS Our results suggest that it may be preferable to continue patients in AKI on factor Xa inhibitors versus transitioning to UFH due to the lower risk of bleeding events.					
36588500	3	5	from	Hospital	405:412	arg1	Michigan					426:433	Michigan	426:433	Michigan	426:433	SETTING Ascension St. John Hospital in Detroit, Michigan.					
36588500	6	6	from	events	921:926	arg1	group					955:959	the factor Xa inhibitor group	931:959	the factor Xa inhibitor group	931:959	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	0	7	with	patients	76:83	arg1	injury					103:108	acute kidney injury	90:108	acute kidney injury	90:108	Risk of bleeding with factor Xa inhibitors versus unfractionated heparin in patients with acute kidney injury.					
36588500	1	8	theme	thromboembolic	151:164	arg1	events					166:171	bleeding and thromboembolic events	138:171	bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI)	138:317	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	0	9	theme	acute	90:94	arg1	injury					103:108	acute kidney injury	90:108	acute kidney injury	90:108	Risk of bleeding with factor Xa inhibitors versus unfractionated heparin in patients with acute kidney injury.					
36588500	4	10	theme	Xa	514:515	arg1	doses					498:502	therapeutic doses	486:502	therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250)	486:553	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.					
36588500	7	11	theme	bleeding	1263:1270	arg1	events					1272:1277	bleeding events	1263:1277	bleeding events	1263:1277	CONCLUSIONS Our results suggest that it may be preferable to continue patients in AKI on factor Xa inhibitors versus transitioning to UFH due to the lower risk of bleeding events.					
36588500	5	12	theme	Xa	698:699	arg1	inhibitor					701:709	a factor Xa inhibitor	689:709	a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events	689:810	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	7	13	theme	events	1272:1277	arg1	risk					1255:1258	the lower risk	1245:1258	the lower risk of bleeding events	1245:1277	CONCLUSIONS Our results suggest that it may be preferable to continue patients in AKI on factor Xa inhibitors versus transitioning to UFH due to the lower risk of bleeding events.					
36588500	6	14	from	differences	1026:1036	arg1	VTE					1083:1085	VTE	1083:1085	VTE	1083:1085	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	6	14	from	differences	1026:1036	arg1	bleeding					1047:1054	bleeding	1047:1054	bleeding	1047:1054	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	6	14	from	differences	1026:1036	arg1	thromboembolism					1066:1080	venous thromboembolism	1059:1080	venous thromboembolism (VTE)	1059:1086	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	4	15	dep	PATIENTS	436:443	arg1	patients					464:471	Hospitalized adult patients	445:471	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.	436:594	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.					
36588500	5	16	theme	%	843:843	arg1	CI					845:846	0.57, 95% CI	835:846	CI	845:846	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	6	17	from	risk	907:910	arg1	group					955:959	the factor Xa inhibitor group	931:959	the factor Xa inhibitor group	931:959	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	7	18	from	patients	1170:1177	arg1	Xa					1196:1197	factor Xa inhibitors versus transitioning	1189:1229	Xa	1196:1197	CONCLUSIONS Our results suggest that it may be preferable to continue patients in AKI on factor Xa inhibitors versus transitioning to UFH due to the lower risk of bleeding events.					
36588500	7	18	from	patients	1170:1177	arg1	transitioning					1217:1229	transitioning	1217:1229	transitioning	1217:1229	CONCLUSIONS Our results suggest that it may be preferable to continue patients in AKI on factor Xa inhibitors versus transitioning to UFH due to the lower risk of bleeding events.					
36588500	7	18	from	patients	1170:1177	arg1	AKI					1182:1184	AKI	1182:1184	AKI	1182:1184	CONCLUSIONS Our results suggest that it may be preferable to continue patients in AKI on factor Xa inhibitors versus transitioning to UFH due to the lower risk of bleeding events.					
36588500	0	19	theme	kidney	96:101	arg1	injury					103:108	acute kidney injury	90:108	acute kidney injury	90:108	Risk of bleeding with factor Xa inhibitors versus unfractionated heparin in patients with acute kidney injury.					
36588500	0	20	from	Xa	29:30	arg1	patients					76:83	patients	76:83	patients with acute kidney injury	76:108	Risk of bleeding with factor Xa inhibitors versus unfractionated heparin in patients with acute kidney injury.					
36588500	6	21	theme	CRNMB	915:919	arg1	events					921:926	CRNMB events	915:926	CRNMB events in the factor Xa inhibitor group	915:959	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	5	22	theme	nonmajor	779:786	arg1	events					805:810	composite major and clinically relevant nonmajor bleeding (CRNMB) events	739:810	composite major and clinically relevant nonmajor bleeding (CRNMB) events	739:810	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	5	23	theme	lower	725:729	arg1	risk					731:734	a lower risk	723:734	a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events	723:810	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	5	24	theme	relevant	770:777	arg1	events					805:810	composite major and clinically relevant nonmajor bleeding (CRNMB) events	739:810	composite major and clinically relevant nonmajor bleeding (CRNMB) events	739:810	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	4	25	theme	therapeutic	486:496	arg1	doses					498:502	therapeutic doses	486:502	therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250)	486:553	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.					
36588500	3	26	theme	Ascension	386:394	arg1	Hospital					405:412	Ascension St. John Hospital	386:412	Ascension St. John Hospital in Detroit, Michigan	386:433	SETTING Ascension St. John Hospital in Detroit, Michigan.					
36588500	6	27	dep	OR	962:963	arg1	p = 0.02					991:998	p = 0.02	991:998	p = 0.02	991:998	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	6	27	dep	OR	962:963	arg1	0.55					966:969	0.55	966:969	0.55	966:969	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	6	27	dep	OR	962:963	arg1	%					974:974	95% CI	972:977	95% CI	972:977	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	6	27	dep	OR	962:963	arg1	0.33-0.91					980:988	0.33-0.91	980:988	0.33-0.91	980:988	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	5	28	theme	factor	691:696	arg1	inhibitor					701:709	a factor Xa inhibitor	689:709	a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events	689:810	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	5	29	theme	bleeding	788:795	arg1	events					805:810	composite major and clinically relevant nonmajor bleeding (CRNMB) events	739:810	composite major and clinically relevant nonmajor bleeding (CRNMB) events	739:810	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	4	30	theme	UFH	541:543	arg1	doses					498:502	therapeutic doses	486:502	therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250)	486:553	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.					
36588500	4	31	dep	Xa	514:515	arg1	n = 250					529:535	n = 250	529:535	n = 250	529:535	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.					
36588500	4	31	dep	Xa	514:515	arg1	inhibitors					517:526	inhibitors	517:526	inhibitors (n = 250)	517:536	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.					
36588500	1	32	with	patients	279:286	arg1	injury					306:311	acute kidney injury	293:311	acute kidney injury (AKI)	293:317	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	1	32	with	patients	279:286	arg1	AKI					314:316	AKI	314:316	AKI	314:316	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	2	33	dep	Single-center	327:339	arg1	retrospective					342:354	retrospective	342:354	retrospective	342:354	DESIGN Single-center, retrospective, observational study.					
36588500	2	33	dep	Single-center	327:339	arg1	observational					357:369	observational	357:369	observational	357:369	DESIGN Single-center, retrospective, observational study.					
36588500	1	34	theme	therapeutic	195:205	arg1	doses					207:211	therapeutic doses	195:211	therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH)	195:274	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	0	35	theme	bleeding	8:15	arg1	Risk					0:3	Risk	0:3	Risk of bleeding with factor Xa inhibitors versus unfractionated heparin in patients with acute kidney injury.	0:109	Risk of bleeding with factor Xa inhibitors versus unfractionated heparin in patients with acute kidney injury.					
36588500	4	36	theme	Hospitalized	445:456	arg1	patients					464:471	Hospitalized adult patients	445:471	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.	436:594	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.					
36588500	2	37	dep	DESIGN	320:325	arg1	study					371:375	Single-center, retrospective, observational study	327:375	DESIGN Single-center, retrospective, observational study	320:375	DESIGN Single-center, retrospective, observational study.					
36588500	4	38	theme	AKI	591:593	arg1	setting					580:586	the setting	576:586	the setting of AKI	576:593	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.					
36588500	1	39	theme	acute	293:297	arg1	injury					306:311	acute kidney injury	293:311	acute kidney injury (AKI)	293:317	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	1	39	theme	acute	293:297	arg1	AKI					314:316	AKI	314:316	AKI	314:316	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	6	40	theme	inhibitor	945:953	arg1	group					955:959	the factor Xa inhibitor group	931:959	the factor Xa inhibitor group	931:959	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	5	41	theme	events	805:810	arg1	risk					731:734	a lower risk	723:734	a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events	723:810	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	0	42	theme	factor	22:27	arg1	Xa					29:30	factor Xa inhibitors versus unfractionated heparin	22:71	Xa	29:30	Risk of bleeding with factor Xa inhibitors versus unfractionated heparin in patients with acute kidney injury.					
36588500	1	43	theme	kidney	299:304	arg1	injury					306:311	acute kidney injury	293:311	acute kidney injury (AKI)	293:317	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	1	43	theme	kidney	299:304	arg1	AKI					314:316	AKI	314:316	AKI	314:316	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	5	44	theme	major	749:753	arg1	events					805:810	composite major and clinically relevant nonmajor bleeding (CRNMB) events	739:810	composite major and clinically relevant nonmajor bleeding (CRNMB) events	739:810	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	5	45	dep	OR	831:832	arg1	CI					845:846	0.57, 95% CI	835:846	CI	845:846	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	5	45	dep	OR	831:832	arg1	p = 0.03					860:867	p = 0.03	860:867	p = 0.03	860:867	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	5	45	dep	OR	831:832	arg1	0.34-0.94					849:857	0.34-0.94	849:857	0.34-0.94	849:857	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	1	46	theme	apixaban	216:223	arg1	doses					207:211	therapeutic doses	195:211	therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH)	195:274	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	3	47	theme	St.	396:398	arg1	Hospital					405:412	Ascension St. John Hospital	386:412	Ascension St. John Hospital in Detroit, Michigan	386:433	SETTING Ascension St. John Hospital in Detroit, Michigan.					
36588500	4	48	from	24 h	568:571	arg1	setting					580:586	the setting	576:586	the setting of AKI	576:593	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.					
36588500	4	49	theme	adult	458:462	arg1	patients					464:471	Hospitalized adult patients	445:471	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.	436:594	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.					
36588500	0	50	dep	Xa	29:30	arg1	inhibitors					32:41	inhibitors	32:41	inhibitors	32:41	Risk of bleeding with factor Xa inhibitors versus unfractionated heparin in patients with acute kidney injury.					
36588500	2	51	theme	Single-center	327:339	arg1	study					371:375	Single-center, retrospective, observational study	327:375	DESIGN Single-center, retrospective, observational study	320:375	DESIGN Single-center, retrospective, observational study.					
36588500	3	52	theme	John	400:403	arg1	Hospital					405:412	Ascension St. John Hospital	386:412	Ascension St. John Hospital in Detroit, Michigan	386:433	SETTING Ascension St. John Hospital in Detroit, Michigan.					
36588500	7	53	dep	CONCLUSIONS	1100:1110	arg1	suggest					1124:1130	suggest	1124:1130	suggest that it may be preferable to continue patients in AKI on factor Xa inhibitors versus transitioning to UFH due to the lower risk of bleeding events	1124:1277	CONCLUSIONS Our results suggest that it may be preferable to continue patients in AKI on factor Xa inhibitors versus transitioning to UFH due to the lower risk of bleeding events.					
36588500	5	54	theme	confounding	646:656	arg1	factors					658:664	confounding factors	646:664	confounding factors	646:664	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	6	55	dep	was	877:879	arg1	OR					962:963	OR	962:963	OR	962:963	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	5	56	dep	UFH	826:828	arg1	OR					831:832	OR	831:832	OR: 0.57, 95% CI: 0.34-0.94; p = 0.03	831:867	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	1	57	theme	STUDY	111:115	arg1	OBJECTIVE					117:125	STUDY OBJECTIVE	111:125	STUDY OBJECTIVE	111:125	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	6	58	from	group	955:959	arg1	risk					907:910	a significantly decreased risk	881:910	a significantly decreased risk of CRNMB events in the factor Xa inhibitor group	881:959	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	7	59	theme	factor	1189:1194	arg1	Xa					1196:1197	factor Xa inhibitors versus transitioning	1189:1229	Xa	1196:1197	CONCLUSIONS Our results suggest that it may be preferable to continue patients in AKI on factor Xa inhibitors versus transitioning to UFH due to the lower risk of bleeding events.					
36588500	0	60	with	Risk	0:3	arg1	Xa					29:30	factor Xa inhibitors versus unfractionated heparin	22:71	Xa	29:30	Risk of bleeding with factor Xa inhibitors versus unfractionated heparin in patients with acute kidney injury.					
36588500	0	60	with	Risk	0:3	arg1	heparin					65:71	unfractionated heparin	50:71	unfractionated heparin	50:71	Risk of bleeding with factor Xa inhibitors versus unfractionated heparin in patients with acute kidney injury.					
36588500	7	61	theme	due	1238:1240	arg1	UFH					1234:1236	UFH	1234:1236	UFH due to the lower risk of bleeding events	1234:1277	CONCLUSIONS Our results suggest that it may be preferable to continue patients in AKI on factor Xa inhibitors versus transitioning to UFH due to the lower risk of bleeding events.					
36588500	6	62	theme	factor	935:940	arg1	group					955:959	the factor Xa inhibitor group	931:959	the factor Xa inhibitor group	931:959	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	6	63	theme	significant	1014:1024	arg1	differences					1026:1036	no significant differences	1011:1036	no significant differences in major bleeding or venous thromboembolism (VTE)	1011:1086	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	6	64	theme	venous	1059:1064	arg1	VTE					1083:1085	VTE	1083:1085	VTE	1083:1085	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	6	64	theme	venous	1059:1064	arg1	thromboembolism					1066:1080	venous thromboembolism	1059:1080	venous thromboembolism (VTE)	1059:1086	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	1	65	theme	rivaroxaban	228:238	arg1	doses					207:211	therapeutic doses	195:211	therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH)	195:274	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	6	66	theme	Xa	942:943	arg1	group					955:959	the factor Xa inhibitor group	931:959	the factor Xa inhibitor group	931:959	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	5	67	theme	CRNMB	798:802	arg1	events					805:810	composite major and clinically relevant nonmajor bleeding (CRNMB) events	739:810	composite major and clinically relevant nonmajor bleeding (CRNMB) events	739:810	MEASUREMENTS AND MAIN RESULTS After adjusting for confounding factors, patients who received a factor Xa inhibitor experienced a lower risk of composite major and clinically relevant nonmajor bleeding (CRNMB) events compared with UFH (OR: 0.57, 95% CI: 0.34-0.94; p = 0.03).					
36588500	0	68	theme	unfractionated	50:63	arg1	heparin					65:71	unfractionated heparin	50:71	unfractionated heparin	50:71	Risk of bleeding with factor Xa inhibitors versus unfractionated heparin in patients with acute kidney injury.					
36588500	4	69	theme	factor	507:512	arg1	Xa					514:515	factor Xa inhibitors (n = 250) or UFH (n = 250)	507:553	Xa	514:515	PATIENTS Hospitalized adult patients who received therapeutic doses of factor Xa inhibitors (n = 250) or UFH (n = 250) for at least 24 h in the setting of AKI.					
36588500	6	70	theme	events	921:926	arg1	risk					907:910	a significantly decreased risk	881:910	a significantly decreased risk of CRNMB events in the factor Xa inhibitor group	881:959	There was a significantly decreased risk of CRNMB events in the factor Xa inhibitor group (OR: 0.55, 95% CI: 0.33-0.91, p = 0.02); however, no significant differences in major bleeding or venous thromboembolism (VTE) were noted.					
36588500	1	71	from	events	166:171	arg1	patients					176:183	patients	176:183	patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI)	176:317	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	1	72	theme	bleeding	138:145	arg1	events					166:171	bleeding and thromboembolic events	138:171	bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI)	138:317	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	1	73	theme	unfractionated	247:260	arg1	UFH					271:273	UFH	271:273	UFH	271:273	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36588500	1	73	theme	unfractionated	247:260	arg1	heparin					262:268	unfractionated heparin	247:268	unfractionated heparin (UFH)	247:274	STUDY OBJECTIVE To compare bleeding and thromboembolic events in patients receiving therapeutic doses of apixaban or rivaroxaban versus unfractionated heparin (UFH) in patients with acute kidney injury (AKI).					
36682201	7	0	theme	oral	1560:1563	arg1	environment					1565:1575	the naturally contaminated oral environment	1533:1575	the naturally contaminated oral environment	1533:1575	Both scaffold types significantly inhibited the growth, attachment and colony formation abilities of S. aureus and E. coli, enhancing the relevance of chitosan in the grafts' composition for the naturally contaminated oral environment.					
36682201	10	1	theme	tissue	2163:2168	arg1	ingrowth					2140:2147	the ingrowth	2136:2147	the ingrowth of connective tissue	2136:2168	The in vivo subcutaneous implantation in mice of both scaffold types showed lower biodegradability with the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks.					
36682201	12	2	theme	ready-to-use	2431:2442	arg1	scaffold					2452:2459	a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold	2389:2459	a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold	2389:2459	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	6	3	theme	bone	1294:1297	arg1	ingrowth					1299:1306	bone ingrowth	1294:1306	bone ingrowth	1294:1306	Dynamic mechanical analysis confirmed their viscoelasticity, but the presence of nHAp significantly enhanced the storage modulus (42.34 ± 6.09 kPa at 10 Hz after 28 days in PBS), showing that it may support bone ingrowth at low-load bearing bone defects.					
36682201	9	4	theme	CS	1956:1957	arg1	samples					1959:1965	the CS samples	1952:1965	the CS samples	1952:1965	MG63 cells seeded within nHAp/CS scaffolds presented a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes compared to the CS samples.					
36682201	12	5	theme	nHAp/CS	2444:2450	arg1	scaffold					2452:2459	a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold	2389:2459	a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold	2389:2459	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	5	6	attach	presence	962:969	arg1	scaffolds					986:994	the scaffolds	982:994	the scaffolds	982:994	The presence of nHAp in the scaffolds promoted a significantly lower biodegradation rate compared to a plain CS scaffold in PBS.					
36682201	5	6	attach	presence	962:969	arg2	nHAp					974:977	nHAp	974:977	nHAp	974:977	The presence of nHAp in the scaffolds promoted a significantly lower biodegradation rate compared to a plain CS scaffold in PBS.					
36682201	12	7	theme	graft	2563:2567	arg1	alternative					2569:2579	a suitable bone graft alternative	2547:2579	it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis	2544:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	10	8	theme	subcutaneous	1980:1991	arg1	implantation					1993:2004	The in vivo subcutaneous implantation	1968:2004	The in vivo subcutaneous implantation in mice of both scaffold types	1968:2035	The in vivo subcutaneous implantation in mice of both scaffold types showed lower biodegradability with the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks.					
36682201	6	9	theme	bone	1328:1331	arg1	defects					1333:1339	low-load bearing bone defects	1311:1339	low-load bearing bone defects	1311:1339	Dynamic mechanical analysis confirmed their viscoelasticity, but the presence of nHAp significantly enhanced the storage modulus (42.34 ± 6.09 kPa at 10 Hz after 28 days in PBS), showing that it may support bone ingrowth at low-load bearing bone defects.					
36682201	3	10	theme	porous	643:648	arg1	microstructure					650:663	the fully interconnected porous microstructure	618:663	the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration	618:774	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	8	11	theme	proliferative	1790:1802	arg1	rate					1804:1807	higher proliferative rate	1783:1807	higher proliferative rate	1783:1807	At SEM and laser scanning confocal microscopy, MG63 cells showed normal morphology and could adhere and proliferate inside the biomaterials' porous structure, especially for the nHAp/CS scaffold, reaching higher proliferative rate at day 14.					
36682201	3	12	dep	scaffold	680:687	arg1	%					708:708	total porosity 78 %	690:708	total porosity 78 %	690:708	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	3	12	dep	scaffold	680:687	arg1	size					723:726	medium pore size 200 μm	711:733	medium pore size 200 μm	711:733	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	12	13	with	production	2375:2384	arg1	features					2525:2532	microstructural, chemical, mechanical and biocompatibility features	2466:2532	microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis	2466:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	11	14	theme	nHAp/CS	2320:2326	arg1	scaffold					2328:2335	the nHAp/CS scaffold	2316:2335	the nHAp/CS scaffold	2316:2335	Histology shows an intensive and progressive ingrowth of new vessels and collagen between the 3rd and the 5th week, especially for the nHAp/CS scaffold.					
36682201	10	15	theme	scaffold	2022:2029	arg1	types					2031:2035	both scaffold types	2017:2035	both scaffold types	2017:2035	The in vivo subcutaneous implantation in mice of both scaffold types showed lower biodegradability with the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks.					
36682201	6	16	theme	low-load	1311:1318	arg1	defects					1333:1339	low-load bearing bone defects	1311:1339	low-load bearing bone defects	1311:1339	Dynamic mechanical analysis confirmed their viscoelasticity, but the presence of nHAp significantly enhanced the storage modulus (42.34 ± 6.09 kPa at 10 Hz after 28 days in PBS), showing that it may support bone ingrowth at low-load bearing bone defects.					
36682201	2	17	theme	nano-hydroxyapatite/chitosan	385:412	arg1	scaffold					431:438	a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold	359:438	a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold	359:438	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	1	18	theme	pathologic	244:253	arg1	defects					269:275	some periodontal pathologic and traumatic defects	227:275	some periodontal pathologic and traumatic defects' size	227:281	Despite bone's innate self-renewal capability, some periodontal pathologic and traumatic defects' size inhibits full spontaneous regeneration.					
36682201	3	19	theme	nHAp/CS	672:678	arg1	scaffold					680:687	the nHAp/CS scaffold	668:687	the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration	668:774	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	3	19	theme	nHAp/CS	672:678	arg1	critical					745:752	critical	745:752	critical	745:752	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	1	20	theme	traumatic	259:267	arg1	defects					269:275	some periodontal pathologic and traumatic defects	227:275	some periodontal pathologic and traumatic defects' size	227:281	Despite bone's innate self-renewal capability, some periodontal pathologic and traumatic defects' size inhibits full spontaneous regeneration.					
36682201	12	21	theme	adverse	2604:2610	arg1	environment					2612:2622	an adverse environment	2601:2622	an adverse environment as in periodontitis and peri-implantitis	2601:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	12	22	theme	suitable	2549:2556	arg1	alternative					2569:2579	a suitable bone graft alternative	2547:2579	it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis	2544:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	7	23	theme	aureus	1446:1451	arg1	abilities					1430:1438	the growth, attachment and colony formation abilities	1386:1438	the growth, attachment and colony formation abilities of S. aureus and E. coli	1386:1463	Both scaffold types significantly inhibited the growth, attachment and colony formation abilities of S. aureus and E. coli, enhancing the relevance of chitosan in the grafts' composition for the naturally contaminated oral environment.					
36682201	3	24	theme	porosity	696:703	arg1	%					708:708	total porosity 78 %	690:708	total porosity 78 %	690:708	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	3	24	theme	porosity	696:703	arg1	size					723:726	medium pore size 200 μm	711:733	medium pore size 200 μm	711:733	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	2	25	theme	current	328:334	arg1	research					336:343	This current research	323:343	This current research	323:343	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	4	26	from	21 days	885:891	arg1	fluid					911:915	simulated body fluid	896:915	simulated body fluid	896:915	Scanning electron microscopy (SEM) showed HAp crystals forming on the surface of the nHAp/CS scaffold after 21 days in simulated body fluid, demonstrating its bioactivity in vitro.					
36682201	6	27	theme	mechanical	1095:1104	arg1	analysis					1106:1113	Dynamic mechanical analysis	1087:1113	Dynamic mechanical analysis	1087:1113	Dynamic mechanical analysis confirmed their viscoelasticity, but the presence of nHAp significantly enhanced the storage modulus (42.34 ± 6.09 kPa at 10 Hz after 28 days in PBS), showing that it may support bone ingrowth at low-load bearing bone defects.					
36682201	7	28	theme	formation	1420:1428	arg1	abilities					1430:1438	the growth, attachment and colony formation abilities	1386:1438	the growth, attachment and colony formation abilities of S. aureus and E. coli	1386:1463	Both scaffold types significantly inhibited the growth, attachment and colony formation abilities of S. aureus and E. coli, enhancing the relevance of chitosan in the grafts' composition for the naturally contaminated oral environment.					
36682201	4	29	theme	Scanning	777:784	arg1	SEM					807:809	SEM	807:809	SEM	807:809	Scanning electron microscopy (SEM) showed HAp crystals forming on the surface of the nHAp/CS scaffold after 21 days in simulated body fluid, demonstrating its bioactivity in vitro.					
36682201	4	29	theme	Scanning	777:784	arg1	microscopy					795:804	Scanning electron microscopy	777:804	Scanning electron microscopy (SEM)	777:810	Scanning electron microscopy (SEM) showed HAp crystals forming on the surface of the nHAp/CS scaffold after 21 days in simulated body fluid, demonstrating its bioactivity in vitro.					
36682201	0	30	theme	CO2	78:80	arg1	scaffold					136:143	a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold	62:143	a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration	62:177	Full physicochemical and biocompatibility characterization of a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration.					
36682201	3	31	theme	medium	711:716	arg1	%					708:708	total porosity 78 %	690:708	total porosity 78 %	690:708	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	3	31	theme	medium	711:716	arg1	size					723:726	medium pore size 200 μm	711:733	medium pore size 200 μm	711:733	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	1	32	theme	innate	195:200	arg1	capability					215:224	bone's innate self-renewal capability	188:224	bone's innate self-renewal capability	188:224	Despite bone's innate self-renewal capability, some periodontal pathologic and traumatic defects' size inhibits full spontaneous regeneration.					
36682201	10	33	theme	structure	2113:2121	arg1	preservation					2076:2087	the preservation	2072:2087	the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks	2072:2182	The in vivo subcutaneous implantation in mice of both scaffold types showed lower biodegradability with the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks.					
36682201	0	34	theme	nano-hydroxyapatite/chitosan	93:120	arg1	scaffold					136:143	a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold	62:143	a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration	62:177	Full physicochemical and biocompatibility characterization of a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration.					
36682201	10	35	theme	scaffolds	2096:2104	arg1	structure					2113:2121	the scaffolds porous structure	2092:2121	the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks	2092:2182	The in vivo subcutaneous implantation in mice of both scaffold types showed lower biodegradability with the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks.					
36682201	0	36	theme	scaffold	136:143	arg1	physicochemical					5:19	Full physicochemical	0:19	Full physicochemical	0:19	Full physicochemical and biocompatibility characterization of a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration.					
36682201	0	36	theme	scaffold	136:143	arg1	characterization					42:57	biocompatibility characterization	25:57	biocompatibility characterization	25:57	Full physicochemical and biocompatibility characterization of a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration.					
36682201	5	37	from	presence	962:969	arg1	scaffolds					986:994	the scaffolds	982:994	the scaffolds	982:994	The presence of nHAp in the scaffolds promoted a significantly lower biodegradation rate compared to a plain CS scaffold in PBS.					
36682201	2	38	theme	preparation	481:491	arg1	method					493:498	preparation method	481:498	preparation method	481:498	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	0	39	theme	periodontal	149:159	arg1	regeneration					166:177	periodontal bone regeneration	149:177	periodontal bone regeneration	149:177	Full physicochemical and biocompatibility characterization of a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration.					
36682201	5	40	theme	plain	1061:1065	arg1	scaffold					1070:1077	a plain CS scaffold	1059:1077	a plain CS scaffold in PBS	1059:1084	The presence of nHAp in the scaffolds promoted a significantly lower biodegradation rate compared to a plain CS scaffold in PBS.					
36682201	7	41	theme	colony	1413:1418	arg1	formation					1420:1428	colony formation	1413:1428	colony formation	1413:1428	Both scaffold types significantly inhibited the growth, attachment and colony formation abilities of S. aureus and E. coli, enhancing the relevance of chitosan in the grafts' composition for the naturally contaminated oral environment.					
36682201	11	42	theme	new	2242:2244	arg1	vessels					2246:2252	new vessels	2242:2252	new vessels	2242:2252	Histology shows an intensive and progressive ingrowth of new vessels and collagen between the 3rd and the 5th week, especially for the nHAp/CS scaffold.					
36682201	8	43	theme	scanning	1595:1602	arg1	microscopy					1613:1622	laser scanning confocal microscopy	1589:1622	laser scanning confocal microscopy	1589:1622	At SEM and laser scanning confocal microscopy, MG63 cells showed normal morphology and could adhere and proliferate inside the biomaterials' porous structure, especially for the nHAp/CS scaffold, reaching higher proliferative rate at day 14.					
36682201	11	44	theme	vessels	2246:2252	arg1	ingrowth					2230:2237	an intensive and progressive ingrowth	2201:2237	an intensive and progressive ingrowth of new vessels and collagen between the 3rd and the 5th week, especially for the nHAp/CS scaffold	2201:2335	Histology shows an intensive and progressive ingrowth of new vessels and collagen between the 3rd and the 5th week, especially for the nHAp/CS scaffold.					
36682201	0	45	theme	Full	0:3	arg1	physicochemical					5:19	Full physicochemical	0:19	Full physicochemical	0:19	Full physicochemical and biocompatibility characterization of a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration.					
36682201	5	46	theme	lower	1021:1025	arg1	rate					1042:1045	a significantly lower biodegradation rate	1005:1045	a significantly lower biodegradation rate compared to a plain CS scaffold in PBS	1005:1084	The presence of nHAp in the scaffolds promoted a significantly lower biodegradation rate compared to a plain CS scaffold in PBS.					
36682201	7	47	theme	attachment	1398:1407	arg1	abilities					1430:1438	the growth, attachment and colony formation abilities	1386:1438	the growth, attachment and colony formation abilities of S. aureus and E. coli	1386:1463	Both scaffold types significantly inhibited the growth, attachment and colony formation abilities of S. aureus and E. coli, enhancing the relevance of chitosan in the grafts' composition for the naturally contaminated oral environment.					
36682201	12	48	theme	biocompatibility	2508:2523	arg1	features					2525:2532	microstructural, chemical, mechanical and biocompatibility features	2466:2532	microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis	2466:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	2	49	theme	final	513:517	arg1	extraction					527:536	the final solvent extraction	509:536	the final solvent extraction	509:536	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	7	50	theme	growth	1390:1395	arg1	abilities					1430:1438	the growth, attachment and colony formation abilities	1386:1438	the growth, attachment and colony formation abilities of S. aureus and E. coli	1386:1463	Both scaffold types significantly inhibited the growth, attachment and colony formation abilities of S. aureus and E. coli, enhancing the relevance of chitosan in the grafts' composition for the naturally contaminated oral environment.					
36682201	9	51	theme	RUNX2	1896:1900	arg1	expression					1882:1891	a higher expression	1873:1891	a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes	1873:1938	MG63 cells seeded within nHAp/CS scaffolds presented a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes compared to the CS samples.					
36682201	8	52	theme	MG63	1625:1628	arg1	cells					1630:1634	MG63 cells	1625:1634	MG63 cells	1625:1634	At SEM and laser scanning confocal microscopy, MG63 cells showed normal morphology and could adhere and proliferate inside the biomaterials' porous structure, especially for the nHAp/CS scaffold, reaching higher proliferative rate at day 14.					
36682201	4	53	theme	simulated	896:904	arg1	fluid					911:915	simulated body fluid	896:915	simulated body fluid	896:915	Scanning electron microscopy (SEM) showed HAp crystals forming on the surface of the nHAp/CS scaffold after 21 days in simulated body fluid, demonstrating its bioactivity in vitro.					
36682201	2	54	theme	supercritical	564:576	arg1	scCO2					583:587	scCO2	583:587	scCO2	583:587	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	2	54	theme	supercritical	564:576	arg1	CO2					578:580	supercritical CO2	564:580	supercritical CO2 (scCO2)	564:588	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	12	55	theme	chemical	2483:2490	arg1	features					2525:2532	microstructural, chemical, mechanical and biocompatibility features	2466:2532	microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis	2466:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	11	56	theme	5th	2291:2293	arg1	week					2295:2298	the 5th week	2287:2298	the 5th week	2287:2298	Histology shows an intensive and progressive ingrowth of new vessels and collagen between the 3rd and the 5th week, especially for the nHAp/CS scaffold.					
36682201	12	57	from	environment	2612:2622	arg1	peri-implantitis					2648:2663	peri-implantitis	2648:2663	peri-implantitis	2648:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	12	57	from	environment	2612:2622	arg1	periodontitis					2630:2642	periodontitis	2630:2642	periodontitis	2630:2642	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	12	58	theme	mechanical	2493:2502	arg1	features					2525:2532	microstructural, chemical, mechanical and biocompatibility features	2466:2532	microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis	2466:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	6	59	dep	modulus	1208:1214	arg1	42.34 ± 6.09 kPa					1217:1232	42.34 ± 6.09 kPa	1217:1232	42.34 ± 6.09 kPa	1217:1232	Dynamic mechanical analysis confirmed their viscoelasticity, but the presence of nHAp significantly enhanced the storage modulus (42.34 ± 6.09 kPa at 10 Hz after 28 days in PBS), showing that it may support bone ingrowth at low-load bearing bone defects.					
36682201	8	60	theme	porous	1719:1724	arg1	structure					1726:1734	the biomaterials' porous structure	1701:1734	the biomaterials' porous structure	1701:1734	At SEM and laser scanning confocal microscopy, MG63 cells showed normal morphology and could adhere and proliferate inside the biomaterials' porous structure, especially for the nHAp/CS scaffold, reaching higher proliferative rate at day 14.					
36682201	6	61	theme	storage	1200:1206	arg1	modulus					1208:1214	the storage modulus	1196:1214	the storage modulus (42.34 ± 6.09 kPa at 10 Hz after 28 days in PBS)	1196:1263	Dynamic mechanical analysis confirmed their viscoelasticity, but the presence of nHAp significantly enhanced the storage modulus (42.34 ± 6.09 kPa at 10 Hz after 28 days in PBS), showing that it may support bone ingrowth at low-load bearing bone defects.					
36682201	9	62	theme	osteogenic	1923:1932	arg1	genes					1934:1938	Sp7 osteogenic genes	1919:1938	Sp7 osteogenic genes	1919:1938	MG63 cells seeded within nHAp/CS scaffolds presented a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes compared to the CS samples.					
36682201	12	63	theme	scaffold	2452:2459	arg1	production					2375:2384	the production	2371:2384	the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis	2371:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	2	64	theme	bone	456:459	arg1	regeneration					461:472	periodontal bone regeneration	444:472	periodontal bone regeneration	444:472	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	1	65	theme	full	292:295	arg1	regeneration					309:320	full spontaneous regeneration	292:320	full spontaneous regeneration	292:320	Despite bone's innate self-renewal capability, some periodontal pathologic and traumatic defects' size inhibits full spontaneous regeneration.					
36682201	9	66	theme	collagen	1903:1910	arg1	A1					1912:1913	collagen A1	1903:1913	collagen A1	1903:1913	MG63 cells seeded within nHAp/CS scaffolds presented a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes compared to the CS samples.					
36682201	7	67	theme	contaminated	1547:1558	arg1	environment					1565:1575	the naturally contaminated oral environment	1533:1575	the naturally contaminated oral environment	1533:1575	Both scaffold types significantly inhibited the growth, attachment and colony formation abilities of S. aureus and E. coli, enhancing the relevance of chitosan in the grafts' composition for the naturally contaminated oral environment.					
36682201	12	68	from	sites	2592:2596	arg1	environment					2612:2622	an adverse environment	2601:2622	an adverse environment as in periodontitis and peri-implantitis	2601:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	12	69	theme	microstructural	2466:2480	arg1	features					2525:2532	microstructural, chemical, mechanical and biocompatibility features	2466:2532	microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis	2466:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	6	70	theme	nHAp	1168:1171	arg1	presence					1156:1163	the presence	1152:1163	the presence of nHAp	1152:1171	Dynamic mechanical analysis confirmed their viscoelasticity, but the presence of nHAp significantly enhanced the storage modulus (42.34 ± 6.09 kPa at 10 Hz after 28 days in PBS), showing that it may support bone ingrowth at low-load bearing bone defects.					
36682201	10	71	theme	connective	2152:2161	arg1	tissue					2163:2168	connective tissue	2152:2168	connective tissue	2152:2168	The in vivo subcutaneous implantation in mice of both scaffold types showed lower biodegradability with the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks.					
36682201	2	72	theme	3D	361:362	arg1	scaffold					431:438	a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold	359:438	a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold	359:438	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	8	73	theme	nHAp/CS	1756:1762	arg1	scaffold					1764:1771	the nHAp/CS scaffold	1752:1771	the nHAp/CS scaffold	1752:1771	At SEM and laser scanning confocal microscopy, MG63 cells showed normal morphology and could adhere and proliferate inside the biomaterials' porous structure, especially for the nHAp/CS scaffold, reaching higher proliferative rate at day 14.					
36682201	5	74	theme	nHAp	974:977	arg1	presence					962:969	The presence	958:969	The presence of nHAp in the scaffolds	958:994	The presence of nHAp in the scaffolds promoted a significantly lower biodegradation rate compared to a plain CS scaffold in PBS.					
36682201	8	75	theme	higher	1783:1788	arg1	rate					1804:1807	higher proliferative rate	1783:1807	higher proliferative rate	1783:1807	At SEM and laser scanning confocal microscopy, MG63 cells showed normal morphology and could adhere and proliferate inside the biomaterials' porous structure, especially for the nHAp/CS scaffold, reaching higher proliferative rate at day 14.					
36682201	3	76	theme	interconnected	628:641	arg1	microstructure					650:663	the fully interconnected porous microstructure	618:663	the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration	618:774	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	11	77	theme	collagen	2258:2265	arg1	ingrowth					2230:2237	an intensive and progressive ingrowth	2201:2237	an intensive and progressive ingrowth of new vessels and collagen between the 3rd and the 5th week, especially for the nHAp/CS scaffold	2201:2335	Histology shows an intensive and progressive ingrowth of new vessels and collagen between the 3rd and the 5th week, especially for the nHAp/CS scaffold.					
36682201	11	78	theme	progressive	2218:2228	arg1	ingrowth					2230:2237	an intensive and progressive ingrowth	2201:2237	an intensive and progressive ingrowth of new vessels and collagen between the 3rd and the 5th week, especially for the nHAp/CS scaffold	2201:2335	Histology shows an intensive and progressive ingrowth of new vessels and collagen between the 3rd and the 5th week, especially for the nHAp/CS scaffold.					
36682201	7	79	theme	chitosan	1493:1500	arg1	relevance					1480:1488	the relevance	1476:1488	the relevance of chitosan in the grafts' composition for the naturally contaminated oral environment	1476:1575	Both scaffold types significantly inhibited the growth, attachment and colony formation abilities of S. aureus and E. coli, enhancing the relevance of chitosan in the grafts' composition for the naturally contaminated oral environment.					
36682201	12	80	theme	defect	2585:2590	arg1	sites					2592:2596	defect sites	2585:2596	defect sites in an adverse environment as in periodontitis and peri-implantitis	2585:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	6	81	theme	bearing	1320:1326	arg1	defects					1333:1339	low-load bearing bone defects	1311:1339	low-load bearing bone defects	1311:1339	Dynamic mechanical analysis confirmed their viscoelasticity, but the presence of nHAp significantly enhanced the storage modulus (42.34 ± 6.09 kPa at 10 Hz after 28 days in PBS), showing that it may support bone ingrowth at low-load bearing bone defects.					
36682201	3	82	theme	scaffold	680:687	arg1	microstructure					650:663	the fully interconnected porous microstructure	618:663	the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration	618:774	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	5	83	from	scaffold	1070:1077	arg1	PBS					1082:1084	PBS	1082:1084	PBS	1082:1084	The presence of nHAp in the scaffolds promoted a significantly lower biodegradation rate compared to a plain CS scaffold in PBS.					
36682201	1	84	theme	periodontal	232:242	arg1	defects					269:275	some periodontal pathologic and traumatic defects	227:275	some periodontal pathologic and traumatic defects' size	227:281	Despite bone's innate self-renewal capability, some periodontal pathologic and traumatic defects' size inhibits full spontaneous regeneration.					
36682201	11	85	theme	intensive	2204:2212	arg1	ingrowth					2230:2237	an intensive and progressive ingrowth	2201:2237	an intensive and progressive ingrowth of new vessels and collagen between the 3rd and the 5th week, especially for the nHAp/CS scaffold	2201:2335	Histology shows an intensive and progressive ingrowth of new vessels and collagen between the 3rd and the 5th week, especially for the nHAp/CS scaffold.					
36682201	2	86	theme	biodegradable	371:383	arg1	nano-hydroxyapatite/chitosan					385:412	porous biodegradable nano-hydroxyapatite/chitosan	364:412	a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold	359:438	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	12	87	theme	bone	2558:2561	arg1	alternative					2569:2579	a suitable bone graft alternative	2547:2579	it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis	2544:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	3	88	theme	78 	705:707	arg1	%					708:708	total porosity 78 %	690:708	total porosity 78 %	690:708	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	3	88	theme	78 	705:707	arg1	size					723:726	medium pore size 200 μm	711:733	medium pore size 200 μm	711:733	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	12	89	theme	cost-effective	2391:2404	arg1	scaffold					2452:2459	a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold	2389:2459	a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold	2389:2459	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	7	90	theme	coli	1460:1463	arg1	abilities					1430:1438	the growth, attachment and colony formation abilities	1386:1438	the growth, attachment and colony formation abilities of S. aureus and E. coli	1386:1463	Both scaffold types significantly inhibited the growth, attachment and colony formation abilities of S. aureus and E. coli, enhancing the relevance of chitosan in the grafts' composition for the naturally contaminated oral environment.					
36682201	10	91	theme	lower	2044:2048	arg1	biodegradability					2050:2065	lower biodegradability	2044:2065	lower biodegradability	2044:2065	The in vivo subcutaneous implantation in mice of both scaffold types showed lower biodegradability with the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks.					
36682201	3	92	theme	total	690:694	arg1	%					708:708	total porosity 78 %	690:708	total porosity 78 %	690:708	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	3	92	theme	total	690:694	arg1	size					723:726	medium pore size 200 μm	711:733	medium pore size 200 μm	711:733	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	10	93	from	implantation	1993:2004	arg1	mice					2009:2012	mice	2009:2012	mice	2009:2012	The in vivo subcutaneous implantation in mice of both scaffold types showed lower biodegradability with the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks.					
36682201	10	94	theme	types	2031:2035	arg1	implantation					1993:2004	The in vivo subcutaneous implantation	1968:2004	The in vivo subcutaneous implantation in mice of both scaffold types	1968:2035	The in vivo subcutaneous implantation in mice of both scaffold types showed lower biodegradability with the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks.					
36682201	4	95	theme	scaffold	870:877	arg1	surface					847:853	the surface	843:853	the surface of the nHAp/CS scaffold	843:877	Scanning electron microscopy (SEM) showed HAp crystals forming on the surface of the nHAp/CS scaffold after 21 days in simulated body fluid, demonstrating its bioactivity in vitro.					
36682201	3	96	theme	pore	718:721	arg1	%					708:708	total porosity 78 %	690:708	total porosity 78 %	690:708	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	3	96	theme	pore	718:721	arg1	size					723:726	medium pore size 200 μm	711:733	medium pore size 200 μm	711:733	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	12	97	dep	it	2544:2545	arg1	alternative					2569:2579	a suitable bone graft alternative	2547:2579	it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis	2544:2663	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	2	98	dep	nano-hydroxyapatite/chitosan	385:412	arg1	70/30					424:428	70/30	424:428	70/30	424:428	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	2	98	dep	nano-hydroxyapatite/chitosan	385:412	arg1	nHAp/CS					415:421	nHAp/CS	415:421	nHAp/CS	415:421	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	0	99	theme	sterilized	82:91	arg1	scaffold					136:143	a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold	62:143	a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration	62:177	Full physicochemical and biocompatibility characterization of a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration.					
36682201	7	100	from	relevance	1480:1488	arg1	composition					1517:1527	the grafts' composition	1505:1527	the grafts' composition for the naturally contaminated oral environment	1505:1575	Both scaffold types significantly inhibited the growth, attachment and colony formation abilities of S. aureus and E. coli, enhancing the relevance of chitosan in the grafts' composition for the naturally contaminated oral environment.					
36682201	6	101	theme	Dynamic	1087:1093	arg1	analysis					1106:1113	Dynamic mechanical analysis	1087:1113	Dynamic mechanical analysis	1087:1113	Dynamic mechanical analysis confirmed their viscoelasticity, but the presence of nHAp significantly enhanced the storage modulus (42.34 ± 6.09 kPa at 10 Hz after 28 days in PBS), showing that it may support bone ingrowth at low-load bearing bone defects.					
36682201	9	102	theme	MG63	1820:1823	arg1	cells					1825:1829	MG63 cells	1820:1829	MG63 cells seeded within nHAp/CS scaffolds	1820:1861	MG63 cells seeded within nHAp/CS scaffolds presented a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes compared to the CS samples.					
36682201	0	103	theme	biodegradable	122:134	arg1	scaffold					136:143	a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold	62:143	a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration	62:177	Full physicochemical and biocompatibility characterization of a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration.					
36682201	1	104	theme	self-renewal	202:213	arg1	capability					215:224	bone's innate self-renewal capability	188:224	bone's innate self-renewal capability	188:224	Despite bone's innate self-renewal capability, some periodontal pathologic and traumatic defects' size inhibits full spontaneous regeneration.					
36682201	4	105	theme	electron	786:793	arg1	SEM					807:809	SEM	807:809	SEM	807:809	Scanning electron microscopy (SEM) showed HAp crystals forming on the surface of the nHAp/CS scaffold after 21 days in simulated body fluid, demonstrating its bioactivity in vitro.					
36682201	4	105	theme	electron	786:793	arg1	microscopy					795:804	Scanning electron microscopy	777:804	Scanning electron microscopy (SEM)	777:810	Scanning electron microscopy (SEM) showed HAp crystals forming on the surface of the nHAp/CS scaffold after 21 days in simulated body fluid, demonstrating its bioactivity in vitro.					
36682201	10	106	theme	porous	2106:2111	arg1	structure					2113:2121	the scaffolds porous structure	2092:2121	the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks	2092:2182	The in vivo subcutaneous implantation in mice of both scaffold types showed lower biodegradability with the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks.					
36682201	2	107	theme	porous	364:369	arg1	nano-hydroxyapatite/chitosan					385:412	porous biodegradable nano-hydroxyapatite/chitosan	364:412	a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold	359:438	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	9	108	theme	nHAp/CS	1845:1851	arg1	scaffolds					1853:1861	nHAp/CS scaffolds	1845:1861	nHAp/CS scaffolds	1845:1861	MG63 cells seeded within nHAp/CS scaffolds presented a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes compared to the CS samples.					
36682201	8	109	theme	laser	1589:1593	arg1	microscopy					1613:1622	laser scanning confocal microscopy	1589:1622	laser scanning confocal microscopy	1589:1622	At SEM and laser scanning confocal microscopy, MG63 cells showed normal morphology and could adhere and proliferate inside the biomaterials' porous structure, especially for the nHAp/CS scaffold, reaching higher proliferative rate at day 14.					
36682201	0	110	theme	bone	161:164	arg1	regeneration					166:177	periodontal bone regeneration	149:177	periodontal bone regeneration	149:177	Full physicochemical and biocompatibility characterization of a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration.					
36682201	8	111	theme	confocal	1604:1611	arg1	microscopy					1613:1622	laser scanning confocal microscopy	1589:1622	laser scanning confocal microscopy	1589:1622	At SEM and laser scanning confocal microscopy, MG63 cells showed normal morphology and could adhere and proliferate inside the biomaterials' porous structure, especially for the nHAp/CS scaffold, reaching higher proliferative rate at day 14.					
36682201	12	112	theme	scCO2	2350:2354	arg1	method					2356:2361	the scCO2 method	2346:2361	the scCO2 method	2346:2361	So far, the scCO2 method enabled the production of a cost-effective and environment-friendly ready-to-use nHAp/CS scaffold with microstructural, chemical, mechanical and biocompatibility features that make it a suitable bone graft alternative for defect sites in an adverse environment as in periodontitis and peri-implantitis.					
36682201	9	113	theme	higher	1875:1880	arg1	expression					1882:1891	a higher expression	1873:1891	a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes	1873:1938	MG63 cells seeded within nHAp/CS scaffolds presented a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes compared to the CS samples.					
36682201	4	114	theme	HAp	819:821	arg1	crystals					823:830	HAp crystals	819:830	HAp crystals forming on the surface of the nHAp/CS scaffold	819:877	Scanning electron microscopy (SEM) showed HAp crystals forming on the surface of the nHAp/CS scaffold after 21 days in simulated body fluid, demonstrating its bioactivity in vitro.					
36682201	6	115	from	10 Hz	1237:1241	arg1	PBS					1260:1262	PBS	1260:1262	PBS	1260:1262	Dynamic mechanical analysis confirmed their viscoelasticity, but the presence of nHAp significantly enhanced the storage modulus (42.34 ± 6.09 kPa at 10 Hz after 28 days in PBS), showing that it may support bone ingrowth at low-load bearing bone defects.					
36682201	6	115	from	10 Hz	1237:1241	arg1	42.34 ± 6.09 kPa					1217:1232	42.34 ± 6.09 kPa	1217:1232	42.34 ± 6.09 kPa	1217:1232	Dynamic mechanical analysis confirmed their viscoelasticity, but the presence of nHAp significantly enhanced the storage modulus (42.34 ± 6.09 kPa at 10 Hz after 28 days in PBS), showing that it may support bone ingrowth at low-load bearing bone defects.					
36682201	0	116	theme	biocompatibility	25:40	arg1	characterization					42:57	biocompatibility characterization	25:57	biocompatibility characterization	25:57	Full physicochemical and biocompatibility characterization of a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration.					
36682201	5	117	theme	biodegradation	1027:1040	arg1	rate					1042:1045	a significantly lower biodegradation rate	1005:1045	a significantly lower biodegradation rate compared to a plain CS scaffold in PBS	1005:1084	The presence of nHAp in the scaffolds promoted a significantly lower biodegradation rate compared to a plain CS scaffold in PBS.					
36682201	2	118	theme	solvent	519:525	arg1	extraction					527:536	the final solvent extraction	509:536	the final solvent extraction	509:536	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	8	119	theme	normal	1643:1648	arg1	morphology					1650:1659	normal morphology	1643:1659	normal morphology	1643:1659	At SEM and laser scanning confocal microscopy, MG63 cells showed normal morphology and could adhere and proliferate inside the biomaterials' porous structure, especially for the nHAp/CS scaffold, reaching higher proliferative rate at day 14.					
36682201	0	120	theme	supercritical	64:76	arg1	scaffold					136:143	a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold	62:143	a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration	62:177	Full physicochemical and biocompatibility characterization of a supercritical CO2 sterilized nano-hydroxyapatite/chitosan biodegradable scaffold for periodontal bone regeneration.					
36682201	7	121	theme	scaffold	1347:1354	arg1	types					1356:1360	Both scaffold types	1342:1360	Both scaffold types	1342:1360	Both scaffold types significantly inhibited the growth, attachment and colony formation abilities of S. aureus and E. coli, enhancing the relevance of chitosan in the grafts' composition for the naturally contaminated oral environment.					
36682201	9	122	theme	A1	1912:1913	arg1	expression					1882:1891	a higher expression	1873:1891	a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes	1873:1938	MG63 cells seeded within nHAp/CS scaffolds presented a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes compared to the CS samples.					
36682201	10	123	dep	in	1972:1973	arg1	vivo					1975:1978	vivo	1975:1978	vivo	1975:1978	The in vivo subcutaneous implantation in mice of both scaffold types showed lower biodegradability with the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks.					
36682201	3	124	theme	bone	758:761	arg1	regeneration					763:774	bone regeneration	758:774	bone regeneration	758:774	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	4	125	theme	body	906:909	arg1	fluid					911:915	simulated body fluid	896:915	simulated body fluid	896:915	Scanning electron microscopy (SEM) showed HAp crystals forming on the surface of the nHAp/CS scaffold after 21 days in simulated body fluid, demonstrating its bioactivity in vitro.					
36682201	3	126	theme	Micro-CT	591:598	arg1	analysis					600:607	Micro-CT analysis	591:607	Micro-CT analysis	591:607	Micro-CT analysis revealed the fully interconnected porous microstructure of the nHAp/CS scaffold (total porosity 78 %, medium pore size 200 μm) which is critical for bone regeneration.					
36682201	5	127	theme	CS	1067:1068	arg1	scaffold					1070:1077	a plain CS scaffold	1059:1077	a plain CS scaffold in PBS	1059:1084	The presence of nHAp in the scaffolds promoted a significantly lower biodegradation rate compared to a plain CS scaffold in PBS.					
36682201	9	128	theme	Sp7	1919:1921	arg1	genes					1934:1938	Sp7 osteogenic genes	1919:1938	Sp7 osteogenic genes	1919:1938	MG63 cells seeded within nHAp/CS scaffolds presented a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes compared to the CS samples.					
36682201	4	129	theme	nHAp/CS	862:868	arg1	scaffold					870:877	the nHAp/CS scaffold	858:877	the nHAp/CS scaffold	858:877	Scanning electron microscopy (SEM) showed HAp crystals forming on the surface of the nHAp/CS scaffold after 21 days in simulated body fluid, demonstrating its bioactivity in vitro.					
36682201	9	130	theme	genes	1934:1938	arg1	expression					1882:1891	a higher expression	1873:1891	a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes	1873:1938	MG63 cells seeded within nHAp/CS scaffolds presented a higher expression of RUNX2, collagen A1 and Sp7 osteogenic genes compared to the CS samples.					
36682201	10	131	theme	in	1972:1973	arg1	implantation					1993:2004	The in vivo subcutaneous implantation	1968:2004	The in vivo subcutaneous implantation in mice of both scaffold types	1968:2035	The in vivo subcutaneous implantation in mice of both scaffold types showed lower biodegradability with the preservation of the scaffolds porous structure that allowed the ingrowth of connective tissue until 5 weeks.					
36682201	2	132	theme	periodontal	444:454	arg1	regeneration					461:472	periodontal bone regeneration	444:472	periodontal bone regeneration	444:472	This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2).					
36682201	1	133	theme	spontaneous	297:307	arg1	regeneration					309:320	full spontaneous regeneration	292:320	full spontaneous regeneration	292:320	Despite bone's innate self-renewal capability, some periodontal pathologic and traumatic defects' size inhibits full spontaneous regeneration.					
36243089	10	0	link	derived	1634:1640	arg1	POX					1642:1644	mangrove actinomycete derived POX	1612:1644	mangrove actinomycete derived POX	1612:1644	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	1	1	theme	novel	293:297	arg1	repertoires					309:319	novel microbial repertoires	293:319	novel microbial repertoires	293:319	Peroxidase (POX) is a heme-containing oxidoreductase, its voluminous immuno-diagnostic and bioremediatory intuitions have incited optimization and large scale-generation from novel microbial repertoires.					
36243089	6	2	with	methodologies	986:998	arg1	temperature					1024:1034	temperature	1024:1034	temperature	1024:1034	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	6	2	with	methodologies	986:998	arg1	casein					1013:1018	casein	1013:1018	casein	1013:1018	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	6	2	with	methodologies	986:998	arg1	starch					1005:1010	starch	1005:1010	starch	1005:1010	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	8	3	theme	casein	1263:1268	arg1	combination					1240:1250	combination	1240:1250	combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively,	1240:1340	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	1	4	theme	voluminous	176:185	arg1	intuitions					224:233	its voluminous immuno-diagnostic and bioremediatory intuitions	172:233	its voluminous immuno-diagnostic and bioremediatory intuitions	172:233	Peroxidase (POX) is a heme-containing oxidoreductase, its voluminous immuno-diagnostic and bioremediatory intuitions have incited optimization and large scale-generation from novel microbial repertoires.					
36243089	8	5	theme	composite	1205:1213	arg1	approach					1215:1222	RSM-BPA composite approach	1197:1222	RSM-BPA composite approach	1197:1222	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	6	6	theme	POX	911:913	arg1	production					915:924	POX production	911:924	POX production	911:924	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	2	7	theme	detrimental	344:354	arg1	dyes					326:329	Azo dyes	322:329	Azo dyes	322:329	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	2	7	theme	detrimental	344:354	arg1	classes					356:362	the most detrimental classes	335:362	the most detrimental classes of synthetic dyes	335:380	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	8	8	dep	7	1358:1358	arg1	folds					1360:1364	folds	1360:1364	folds enhancement of POX outturn (2.52 U/mL)	1360:1403	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	9	9	with	dyes	1517:1520	arg1	10 mM					1535:1539	10 mM	1535:1539	10 mM	1535:1539	The concentrated enzyme decolorized 75.4% and 90% of the two azo dyes with lignin (10 mM), respectively.					
36243089	9	9	with	dyes	1517:1520	arg1	lignin					1527:1532	lignin	1527:1532	lignin (10 mM)	1527:1540	The concentrated enzyme decolorized 75.4% and 90% of the two azo dyes with lignin (10 mM), respectively.					
36243089	1	10	from	repertoires	309:319	arg1	optimization					248:259	optimization	248:259	optimization	248:259	Peroxidase (POX) is a heme-containing oxidoreductase, its voluminous immuno-diagnostic and bioremediatory intuitions have incited optimization and large scale-generation from novel microbial repertoires.					
36243089	1	10	from	repertoires	309:319	arg1	scale-generation					271:286	large scale-generation	265:286	large scale-generation	265:286	Peroxidase (POX) is a heme-containing oxidoreductase, its voluminous immuno-diagnostic and bioremediatory intuitions have incited optimization and large scale-generation from novel microbial repertoires.					
36243089	3	11	theme	recalcitrant	586:597	arg1	traits					599:604	recalcitrant traits	586:604	recalcitrant traits	586:604	In addition, azo dyes are refractory to degradation owing to their chemical nature, comprising of azoic linkages, amino moieties with recalcitrant traits.					
36243089	8	12	dep	resulted	1346:1353	arg1	compared					1405:1412	compared	1405:1412	compared to the unoptimized media (0.36 U/mL)	1405:1449	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	6	13	theme	Placket-Burman	971:984	arg1	methodologies					986:998	One Factor at a Time and Placket-Burman methodologies	946:998	One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature	946:1034	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	6	13	theme	Placket-Burman	971:984	arg1	parameters					1049:1058	essential parameters	1039:1058	essential parameters	1039:1058	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	3	14	theme	azo	465:467	arg1	dyes					469:472	azo dyes	465:472	azo dyes	465:472	In addition, azo dyes are refractory to degradation owing to their chemical nature, comprising of azoic linkages, amino moieties with recalcitrant traits.					
36243089	8	15	theme	starch	1255:1260	arg1	combination					1240:1250	combination	1240:1250	combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively,	1240:1340	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	0	16	from	SPR7	71:74	arg1	peroxidase					24:33	novel peroxidase	18:33	novel peroxidase from Streptomyces coelicolor strain SPR7	18:74	Bioprospecting of novel peroxidase from Streptomyces coelicolor strain SPR7 for carcinogenic azo dyes decolorization.					
36243089	8	17	theme	%	1308:1308	arg1	values					1298:1303	optimal values	1290:1303	optimal values 2.5%	1290:1308	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	7	18	theme	Response	1075:1082	arg1	Methodology					1092:1102	Response Surface Methodology	1075:1102	Response Surface Methodology (RSM)	1075:1108	In auxiliary, Response Surface Methodology (RSM) was recruited and followed by model validation using Back propagation algorithm (BPA).					
36243089	7	18	theme	Response	1075:1082	arg1	RSM					1105:1107	RSM	1105:1107	RSM	1105:1107	In auxiliary, Response Surface Methodology (RSM) was recruited and followed by model validation using Back propagation algorithm (BPA).					
36243089	9	19	theme	concentrated	1456:1467	arg1	enzyme					1469:1474	The concentrated enzyme	1452:1474	The concentrated enzyme	1452:1474	The concentrated enzyme decolorized 75.4% and 90% of the two azo dyes with lignin (10 mM), respectively.					
36243089	10	20	theme	carcinogenic	1700:1711	arg1	effects					1740:1746	their carcinogenic, mutagenic and teratogenic effects	1694:1746	their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms	1694:1778	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	8	21	from	values	1298:1303	arg1	35 °C					1322:1326	35 °C	1322:1326	35 °C	1322:1326	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	8	21	from	values	1298:1303	arg1	casein					1263:1268	casein	1263:1268	casein	1263:1268	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	8	21	from	values	1298:1303	arg1	combination					1240:1250	combination	1240:1250	combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively,	1240:1340	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	8	21	from	values	1298:1303	arg1	temperature					1275:1285	temperature	1275:1285	temperature	1275:1285	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	8	21	from	values	1298:1303	arg1	starch					1255:1260	starch	1255:1260	starch	1255:1260	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	8	21	from	values	1298:1303	arg1	%					1316:1316	0.035%	1311:1316	0.035%	1311:1316	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	5	22	theme	Streptomyces	770:781	arg1	coelicolor					783:792	Streptomyces coelicolor	770:792	Streptomyces coelicolor strain SPR7	770:804	In the present study, a novel POX from Streptomyces coelicolor strain SPR7 was investigated for the deterioration of ecotoxic dyestuffs.					
36243089	10	23	theme	aquatic	1762:1768	arg1	organisms					1770:1778	aquatic organisms	1762:1778	aquatic organisms	1762:1778	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	1	24	theme	heme-containing	140:154	arg1	oxidoreductase					156:169	a heme-containing oxidoreductase	138:169	a heme-containing oxidoreductase	138:169	Peroxidase (POX) is a heme-containing oxidoreductase, its voluminous immuno-diagnostic and bioremediatory intuitions have incited optimization and large scale-generation from novel microbial repertoires.					
36243089	1	24	theme	heme-containing	140:154	arg1	Peroxidase					118:127	Peroxidase	118:127	Peroxidase (POX)	118:133	Peroxidase (POX) is a heme-containing oxidoreductase, its voluminous immuno-diagnostic and bioremediatory intuitions have incited optimization and large scale-generation from novel microbial repertoires.					
36243089	0	25	theme	carcinogenic	80:91	arg1	dyes					97:100	carcinogenic azo dyes	80:100	carcinogenic azo dyes	80:100	Bioprospecting of novel peroxidase from Streptomyces coelicolor strain SPR7 for carcinogenic azo dyes decolorization.					
36243089	5	26	from	SPR7	801:804	arg1	POX					761:763	a novel POX	753:763	a novel POX from Streptomyces coelicolor strain SPR7	753:804	In the present study, a novel POX from Streptomyces coelicolor strain SPR7 was investigated for the deterioration of ecotoxic dyestuffs.					
36243089	2	27	from	classes	356:362	arg1	wastewater					440:449	wastewater	440:449	wastewater	440:449	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	0	28	theme	Streptomyces	40:51	arg1	coelicolor					53:62	Streptomyces coelicolor	40:62	Streptomyces coelicolor strain SPR7	40:74	Bioprospecting of novel peroxidase from Streptomyces coelicolor strain SPR7 for carcinogenic azo dyes decolorization.					
36243089	3	29	theme	azoic	550:554	arg1	linkages					556:563	azoic linkages	550:563	azoic linkages	550:563	In addition, azo dyes are refractory to degradation owing to their chemical nature, comprising of azoic linkages, amino moieties with recalcitrant traits.					
36243089	3	29	theme	azoic	550:554	arg1	moieties					572:579	amino moieties	566:579	amino moieties with recalcitrant traits	566:604	In addition, azo dyes are refractory to degradation owing to their chemical nature, comprising of azoic linkages, amino moieties with recalcitrant traits.					
36243089	2	30	theme	industrial	415:424	arg1	pollutants					426:435	the common ecotoxic industrial pollutants	395:435	the common ecotoxic industrial pollutants in wastewater	395:449	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	2	30	theme	industrial	415:424	arg1	they					386:389	they	386:389	they	386:389	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	9	31	theme	dyes	1517:1520	arg1	%					1492:1492	75.4%	1488:1492	75.4%	1488:1492	The concentrated enzyme decolorized 75.4% and 90% of the two azo dyes with lignin (10 mM), respectively.					
36243089	9	31	theme	dyes	1517:1520	arg1	%					1500:1500	90%	1498:1500	90% of the two azo dyes with lignin (10 mM)	1498:1540	The concentrated enzyme decolorized 75.4% and 90% of the two azo dyes with lignin (10 mM), respectively.					
36243089	9	31	theme	dyes	1517:1520	arg1	dyes					1517:1520	the two azo dyes	1505:1520	the two azo dyes with lignin (10 mM)	1505:1540	The concentrated enzyme decolorized 75.4% and 90% of the two azo dyes with lignin (10 mM), respectively.					
36243089	10	32	theme	actinomycete	1621:1632	arg1	POX					1642:1644	mangrove actinomycete derived POX	1612:1644	mangrove actinomycete derived POX	1612:1644	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	0	33	theme	novel	18:22	arg1	peroxidase					24:33	novel peroxidase	18:33	novel peroxidase from Streptomyces coelicolor strain SPR7	18:74	Bioprospecting of novel peroxidase from Streptomyces coelicolor strain SPR7 for carcinogenic azo dyes decolorization.					
36243089	10	34	theme	dyes	1677:1680	arg1	elimination					1650:1660	elimination	1650:1660	elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms	1650:1778	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	8	35	theme	outturn	1385:1391	arg1	enhancement					1366:1376	enhancement	1366:1376	enhancement of POX outturn (2.52 U/mL)	1366:1403	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	6	36	theme	component	887:895	arg1	screening					897:905	The initial medium component screening	868:905	The initial medium component screening for POX production	868:924	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	3	37	theme	chemical	519:526	arg1	nature					528:533	their chemical nature	513:533	their chemical nature	513:533	In addition, azo dyes are refractory to degradation owing to their chemical nature, comprising of azoic linkages, amino moieties with recalcitrant traits.					
36243089	3	38	with	moieties	572:579	arg1	traits					599:604	recalcitrant traits	586:604	recalcitrant traits	586:604	In addition, azo dyes are refractory to degradation owing to their chemical nature, comprising of azoic linkages, amino moieties with recalcitrant traits.					
36243089	6	39	theme	initial	872:878	arg1	screening					897:905	The initial medium component screening	868:905	The initial medium component screening for POX production	868:924	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	2	40	theme	synthetic	367:375	arg1	dyes					377:380	synthetic dyes	367:380	synthetic dyes	367:380	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	5	41	theme	ecotoxic	848:855	arg1	dyestuffs					857:865	ecotoxic dyestuffs	848:865	ecotoxic dyestuffs	848:865	In the present study, a novel POX from Streptomyces coelicolor strain SPR7 was investigated for the deterioration of ecotoxic dyestuffs.					
36243089	2	42	theme	common	399:404	arg1	pollutants					426:435	the common ecotoxic industrial pollutants	395:435	the common ecotoxic industrial pollutants in wastewater	395:449	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	2	42	theme	common	399:404	arg1	they					386:389	they	386:389	they	386:389	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	1	43	theme	large	265:269	arg1	scale-generation					271:286	large scale-generation	265:286	large scale-generation	265:286	Peroxidase (POX) is a heme-containing oxidoreductase, its voluminous immuno-diagnostic and bioremediatory intuitions have incited optimization and large scale-generation from novel microbial repertoires.					
36243089	6	44	theme	Factor	950:955	arg1	methodologies					986:998	One Factor at a Time and Placket-Burman methodologies	946:998	One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature	946:1034	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	6	44	theme	Factor	950:955	arg1	parameters					1049:1058	essential parameters	1039:1058	essential parameters	1039:1058	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	5	45	theme	coelicolor	783:792	arg1	SPR7					801:804	Streptomyces coelicolor strain SPR7	770:804	Streptomyces coelicolor strain SPR7	770:804	In the present study, a novel POX from Streptomyces coelicolor strain SPR7 was investigated for the deterioration of ecotoxic dyestuffs.					
36243089	7	46	theme	model	1140:1144	arg1	validation					1146:1155	model validation	1140:1155	model validation using Back propagation algorithm (BPA)	1140:1194	In auxiliary, Response Surface Methodology (RSM) was recruited and followed by model validation using Back propagation algorithm (BPA).					
36243089	10	47	theme	noxious	1665:1671	arg1	dyes					1677:1680	noxious azo dyes	1665:1680	noxious azo dyes	1665:1680	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	3	48	theme	amino	566:570	arg1	linkages					556:563	azoic linkages	550:563	azoic linkages	550:563	In addition, azo dyes are refractory to degradation owing to their chemical nature, comprising of azoic linkages, amino moieties with recalcitrant traits.					
36243089	3	48	theme	amino	566:570	arg1	moieties					572:579	amino moieties	566:579	amino moieties with recalcitrant traits	566:604	In addition, azo dyes are refractory to degradation owing to their chemical nature, comprising of azoic linkages, amino moieties with recalcitrant traits.					
36243089	1	49	theme	microbial	299:307	arg1	repertoires					309:319	novel microbial repertoires	293:319	novel microbial repertoires	293:319	Peroxidase (POX) is a heme-containing oxidoreductase, its voluminous immuno-diagnostic and bioremediatory intuitions have incited optimization and large scale-generation from novel microbial repertoires.					
36243089	2	50	theme	Azo	322:324	arg1	dyes					326:329	Azo dyes	322:329	Azo dyes	322:329	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	2	50	theme	Azo	322:324	arg1	classes					356:362	the most detrimental classes	335:362	the most detrimental classes of synthetic dyes	335:380	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	0	51	theme	coelicolor	53:62	arg1	SPR7					71:74	Streptomyces coelicolor strain SPR7	40:74	Streptomyces coelicolor strain SPR7	40:74	Bioprospecting of novel peroxidase from Streptomyces coelicolor strain SPR7 for carcinogenic azo dyes decolorization.					
36243089	8	52	from	combination	1240:1250	arg1	values					1298:1303	optimal values	1290:1303	optimal values 2.5%	1290:1308	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	5	53	theme	strain	794:799	arg1	SPR7					801:804	Streptomyces coelicolor strain SPR7	770:804	Streptomyces coelicolor strain SPR7	770:804	In the present study, a novel POX from Streptomyces coelicolor strain SPR7 was investigated for the deterioration of ecotoxic dyestuffs.					
36243089	10	54	theme	teratogenic	1728:1738	arg1	effects					1740:1746	their carcinogenic, mutagenic and teratogenic effects	1694:1746	their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms	1694:1778	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	1	55	theme	immuno-diagnostic	187:203	arg1	intuitions					224:233	its voluminous immuno-diagnostic and bioremediatory intuitions	172:233	its voluminous immuno-diagnostic and bioremediatory intuitions	172:233	Peroxidase (POX) is a heme-containing oxidoreductase, its voluminous immuno-diagnostic and bioremediatory intuitions have incited optimization and large scale-generation from novel microbial repertoires.					
36243089	8	56	theme	RSM-BPA	1197:1203	arg1	approach					1215:1222	RSM-BPA composite approach	1197:1222	RSM-BPA composite approach	1197:1222	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	10	57	theme	mutagenic	1714:1722	arg1	effects					1740:1746	their carcinogenic, mutagenic and teratogenic effects	1694:1746	their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms	1694:1778	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	1	58	theme	bioremediatory	209:222	arg1	intuitions					224:233	its voluminous immuno-diagnostic and bioremediatory intuitions	172:233	its voluminous immuno-diagnostic and bioremediatory intuitions	172:233	Peroxidase (POX) is a heme-containing oxidoreductase, its voluminous immuno-diagnostic and bioremediatory intuitions have incited optimization and large scale-generation from novel microbial repertoires.					
36243089	8	59	theme	unoptimized	1421:1431	arg1	media					1433:1437	the unoptimized media	1417:1437	the unoptimized media (0.36 U/mL)	1417:1449	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	8	59	theme	unoptimized	1421:1431	arg1	U/mL					1445:1448	0.36 U/mL	1440:1448	0.36 U/mL	1440:1448	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	8	60	theme	temperature	1275:1285	arg1	combination					1240:1250	combination	1240:1250	combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively,	1240:1340	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	7	61	theme	Surface	1084:1090	arg1	Methodology					1092:1102	Response Surface Methodology	1075:1102	Response Surface Methodology (RSM)	1075:1108	In auxiliary, Response Surface Methodology (RSM) was recruited and followed by model validation using Back propagation algorithm (BPA).					
36243089	7	61	theme	Surface	1084:1090	arg1	RSM					1105:1107	RSM	1105:1107	RSM	1105:1107	In auxiliary, Response Surface Methodology (RSM) was recruited and followed by model validation using Back propagation algorithm (BPA).					
36243089	8	62	theme	optimal	1290:1296	arg1	values					1298:1303	optimal values	1290:1303	optimal values 2.5%	1290:1308	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	8	63	theme	2.5	1305:1307	arg1	%					1308:1308	%	1308:1308	%	1308:1308	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	10	64	theme	POX	1642:1644	arg1	potentiality					1596:1607	the potentiality	1592:1607	the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms	1592:1778	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	5	65	theme	dyestuffs	857:865	arg1	deterioration					831:843	the deterioration	827:843	the deterioration of ecotoxic dyestuffs	827:865	In the present study, a novel POX from Streptomyces coelicolor strain SPR7 was investigated for the deterioration of ecotoxic dyestuffs.					
36243089	6	66	theme	essential	1039:1047	arg1	methodologies					986:998	One Factor at a Time and Placket-Burman methodologies	946:998	One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature	946:1034	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	6	66	theme	essential	1039:1047	arg1	parameters					1049:1058	essential parameters	1039:1058	essential parameters	1039:1058	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	0	67	theme	azo	93:95	arg1	dyes					97:100	carcinogenic azo dyes	80:100	carcinogenic azo dyes	80:100	Bioprospecting of novel peroxidase from Streptomyces coelicolor strain SPR7 for carcinogenic azo dyes decolorization.					
36243089	3	68	from	addition	455:462	arg1	refractory					478:487	refractory	478:487	refractory	478:487	In addition, azo dyes are refractory to degradation owing to their chemical nature, comprising of azoic linkages, amino moieties with recalcitrant traits.					
36243089	5	69	theme	novel	755:759	arg1	POX					761:763	a novel POX	753:763	a novel POX from Streptomyces coelicolor strain SPR7	753:804	In the present study, a novel POX from Streptomyces coelicolor strain SPR7 was investigated for the deterioration of ecotoxic dyestuffs.					
36243089	0	70	dep	decolorization	102:115	arg1	Bioprospecting					0:13	Bioprospecting	0:13	Bioprospecting	0:13	Bioprospecting of novel peroxidase from Streptomyces coelicolor strain SPR7 for carcinogenic azo dyes decolorization.					
36243089	10	71	from	effects	1740:1746	arg1	humans					1751:1756	humans	1751:1756	humans	1751:1756	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	10	71	from	effects	1740:1746	arg1	organisms					1770:1778	aquatic organisms	1762:1778	aquatic organisms	1762:1778	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	9	72	theme	azo	1513:1515	arg1	dyes					1517:1520	the two azo dyes	1505:1520	the two azo dyes with lignin (10 mM)	1505:1540	The concentrated enzyme decolorized 75.4% and 90% of the two azo dyes with lignin (10 mM), respectively.					
36243089	5	73	theme	present	738:744	arg1	study					746:750	the present study	734:750	the present study	734:750	In the present study, a novel POX from Streptomyces coelicolor strain SPR7 was investigated for the deterioration of ecotoxic dyestuffs.					
36243089	2	74	theme	ecotoxic	406:413	arg1	pollutants					426:435	the common ecotoxic industrial pollutants	395:435	the common ecotoxic industrial pollutants in wastewater	395:449	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	2	74	theme	ecotoxic	406:413	arg1	they					386:389	they	386:389	they	386:389	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	10	75	theme	mangrove	1612:1619	arg1	POX					1642:1644	mangrove actinomycete derived POX	1612:1644	mangrove actinomycete derived POX	1612:1644	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	7	76	theme	propagation	1168:1178	arg1	BPA					1191:1193	BPA	1191:1193	BPA	1191:1193	In auxiliary, Response Surface Methodology (RSM) was recruited and followed by model validation using Back propagation algorithm (BPA).					
36243089	7	76	theme	propagation	1168:1178	arg1	algorithm					1180:1188	Back propagation algorithm	1163:1188	Back propagation algorithm (BPA)	1163:1194	In auxiliary, Response Surface Methodology (RSM) was recruited and followed by model validation using Back propagation algorithm (BPA).					
36243089	8	77	theme	POX	1381:1383	arg1	outturn					1385:1391	POX outturn	1381:1391	POX outturn (2.52 U/mL)	1381:1403	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	8	77	theme	POX	1381:1383	arg1	U/mL					1399:1402	2.52 U/mL	1394:1402	2.52 U/mL	1394:1402	RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL).					
36243089	3	78	from	refractory	478:487	arg1	addition					455:462	addition	455:462	addition	455:462	In addition, azo dyes are refractory to degradation owing to their chemical nature, comprising of azoic linkages, amino moieties with recalcitrant traits.					
36243089	0	79	theme	strain	64:69	arg1	SPR7					71:74	Streptomyces coelicolor strain SPR7	40:74	Streptomyces coelicolor strain SPR7	40:74	Bioprospecting of novel peroxidase from Streptomyces coelicolor strain SPR7 for carcinogenic azo dyes decolorization.					
36243089	6	80	theme	medium	880:885	arg1	screening					897:905	The initial medium component screening	868:905	The initial medium component screening for POX production	868:924	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	2	81	theme	dyes	377:380	arg1	pollutants					426:435	the common ecotoxic industrial pollutants	395:435	the common ecotoxic industrial pollutants in wastewater	395:449	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	2	81	theme	dyes	377:380	arg1	dyes					326:329	Azo dyes	322:329	Azo dyes	322:329	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	2	81	theme	dyes	377:380	arg1	they					386:389	they	386:389	they	386:389	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	2	81	theme	dyes	377:380	arg1	classes					356:362	the most detrimental classes	335:362	the most detrimental classes of synthetic dyes	335:380	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	2	82	from	pollutants	426:435	arg1	wastewater					440:449	wastewater	440:449	wastewater	440:449	Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater.					
36243089	6	83	from	Time	962:965	arg1	Placket-Burman					971:984	Placket-Burman	971:984	Placket-Burman	971:984	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	6	83	from	Time	962:965	arg1	Factor					950:955	One Factor	946:955	One Factor at a Time	946:965	The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters.					
36243089	10	84	theme	derived	1634:1640	arg1	POX					1642:1644	mangrove actinomycete derived POX	1612:1644	mangrove actinomycete derived POX	1612:1644	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36243089	10	85	theme	azo	1673:1675	arg1	dyes					1677:1680	noxious azo dyes	1665:1680	noxious azo dyes	1665:1680	Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms.					
36860098	0	0	theme	pharmacological	168:182	arg1	interventions					197:209	pharmacological prophylactic interventions	168:209	pharmacological prophylactic interventions	168:209	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	2	1	theme	anti-thrombotic	380:394	arg1	regimens					396:403	two different anti-thrombotic regimens	366:403	two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia	366:468	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	6	2	with	newborns	1103:1110	arg1	%					1141:1141	17%	1139:1141	17%	1139:1141	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	2	with	newborns	1103:1110	arg1	weight					1123:1128	birth weight <2500 g	1117:1136	birth weight <2500 g (17%)	1117:1142	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	2	3	from	efficacy	354:361	arg1	women					408:412	women	408:412	women with a history of late fetal loss without thrombophilia	408:468	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	5	4	with	prophylaxis	836:846	arg1	acid					869:872	acetylsalicylic acid	853:872	acetylsalicylic acid (ASA)	853:878	In their subsequent pregnancies, 55 received prophylaxis with acetylsalicylic acid (ASA) only and 73 received ASA plus low molecular weight heparin (LMWH).					
36860098	5	4	with	prophylaxis	836:846	arg1	ASA					875:877	ASA	875:877	ASA	875:877	In their subsequent pregnancies, 55 received prophylaxis with acetylsalicylic acid (ASA) only and 73 received ASA plus low molecular weight heparin (LMWH).					
36860098	6	5	dep	RESULTS	947:953	arg1	had					999:1001	had	999:1001	had adverse outcomes related to placental dysfunction	999:1051	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	5	dep	RESULTS	947:953	arg1	births					1063:1068	pre-term births	1054:1068	pre-term births (25% <37 weeks, 5.6% <34 weeks)	1054:1100	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	5	dep	RESULTS	947:953	arg1	newborns					1103:1110	newborns	1103:1110	newborns with birth weight <2500 g (17%)	1103:1142	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	5	dep	RESULTS	947:953	arg1	newborns					1149:1156	newborns	1149:1156	newborns small for gestational age (5%)	1149:1187	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	5	dep	RESULTS	947:953	arg1	<37 weeks					1075:1083	25% <37 weeks	1071:1083	25% <37 weeks	1071:1083	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	0	6	theme	interventions	197:209	arg1	risk					133:136	risk	133:136	risk of recurrence	133:150	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	6	theme	interventions	197:209	arg1	complications					28:40	Placenta-mediated pregnancy complications	0:40	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.	0:210	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	6	theme	interventions	197:209	arg1	efficacy					156:163	efficacy	156:163	efficacy of pharmacological prophylactic interventions	156:209	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	2	7	theme	placenta-mediated	296:312	arg1	complications					324:336	severe placenta-mediated pregnancy complications	289:336	severe placenta-mediated pregnancy complications	289:336	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	8	8	dep	RR	1659:1660	arg1	%					1670:1670	95%CI: 0.22-1.19, p = 0.1242	1668:1695	%	1670:1670	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	12	9	located	detected	2116:2123	arg2	reduction					2068:2076	A reduction	2066:2076	A reduction of the risk of delivery <34 weeks	2066:2110	A reduction of the risk of delivery <34 weeks was detected in the ASA plus LMWH group.					
36860098	12	9	located	detected	2116:2123	arg1	group					2146:2150	the ASA plus LMWH group	2128:2150	group	2146:2150	A reduction of the risk of delivery <34 weeks was detected in the ASA plus LMWH group.					
36860098	9	10	theme	absolute	1702:1709	arg1	reduction					1716:1724	An absolute risk reduction	1699:1724	An absolute risk reduction of 5.31%	1699:1733	An absolute risk reduction of 5.31% was observed for the ASA plus LMWH group.					
36860098	5	11	theme	low	910:912	arg1	heparin					931:937	low molecular weight heparin	910:937	low molecular weight heparin (LMWH)	910:944	In their subsequent pregnancies, 55 received prophylaxis with acetylsalicylic acid (ASA) only and 73 received ASA plus low molecular weight heparin (LMWH).					
36860098	5	11	theme	low	910:912	arg1	LMWH					940:943	LMWH	940:943	LMWH	940:943	In their subsequent pregnancies, 55 received prophylaxis with acetylsalicylic acid (ASA) only and 73 received ASA plus low molecular weight heparin (LMWH).					
36860098	11	12	from	CONCLUSION	1888:1897	arg1	substantial					1995:2005	substantial	1995:2005	substantial	1995:2005	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	11	12	from	CONCLUSION	1888:1897	arg1	risk					1928:1931	the risk	1924:1931	the risk of recurrence of placenta-mediated pregnancy complications	1924:1990	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	2	13	theme	complications	324:336	arg1	recurrence					275:284	recurrence	275:284	recurrence of severe placenta-mediated pregnancy complications	275:336	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	1	14	theme	10-year	214:220	arg1	study					236:240	A 10-year retrospective study	212:240	A 10-year retrospective study.	212:241	A 10-year retrospective study.					
36860098	12	15	theme	risk	2085:2088	arg1	reduction					2068:2076	A reduction	2066:2076	A reduction of the risk of delivery <34 weeks	2066:2110	A reduction of the risk of delivery <34 weeks was detected in the ASA plus LMWH group.					
36860098	11	16	theme	thrombophilic	2040:2052	arg1	conditions					2054:2063	maternal thrombophilic conditions	2031:2063	maternal thrombophilic conditions	2031:2063	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	12	17	theme	delivery	2093:2100	arg1	<34 weeks					2102:2110	delivery <34 weeks	2093:2110	delivery <34 weeks	2093:2110	A reduction of the risk of delivery <34 weeks was detected in the ASA plus LMWH group.					
36860098	0	18	theme	recurrence	141:150	arg1	risk					133:136	risk	133:136	risk of recurrence	133:150	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	18	theme	recurrence	141:150	arg1	complications					28:40	Placenta-mediated pregnancy complications	0:40	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.	0:210	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	18	theme	recurrence	141:150	arg1	efficacy					156:163	efficacy	156:163	efficacy of pharmacological prophylactic interventions	156:209	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	3	19	theme	fetal	621:625	arg1	loss					627:630	pregnancy fetal loss	611:630	pregnancy fetal loss	611:630	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	8	20	theme	combination	1352:1362	arg1	therapy					1364:1370	combination therapy	1352:1370	combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045)	1352:1472	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	6	21	contain	had	999:1001	arg1	one-third					964:972	one-third	964:972	one-third	964:972	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	21	contain	had	999:1001	arg1	pregnancies					981:991	all pregnancies	977:991	all pregnancies (31%)	977:997	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	21	contain	had	999:1001	arg1	%					996:996	31%	994:996	31%	994:996	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	21	contain	had	999:1001	arg2	outcomes					1011:1018	adverse outcomes	1003:1018	adverse outcomes related to placental dysfunction	1003:1051	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	3	22	theme	age	658:660	arg1	>20 weeks					633:641	>20 weeks	633:641	>20 weeks of gestational age	633:660	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	11	23	theme	pregnancy	1968:1976	arg1	complications					1978:1990	placenta-mediated pregnancy complications	1950:1990	placenta-mediated pregnancy complications	1950:1990	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	7	24	theme	loss	1273:1276	arg1	%					1294:1294	6%	1293:1294	6%	1293:1294	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks were 6%, 5%, and 4% respectively.					
36860098	7	24	theme	loss	1273:1276	arg1	prevalence					1194:1203	The prevalence	1190:1203	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks	1190:1286	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks were 6%, 5%, and 4% respectively.					
36860098	9	25	theme	ASA	1756:1758	arg1	group					1770:1774	the ASA plus LMWH group	1752:1774	group	1770:1774	An absolute risk reduction of 5.31% was observed for the ASA plus LMWH group.					
36860098	5	26	theme	subsequent	800:809	arg1	pregnancies					811:821	their subsequent pregnancies	794:821	their subsequent pregnancies	794:821	In their subsequent pregnancies, 55 received prophylaxis with acetylsalicylic acid (ASA) only and 73 received ASA plus low molecular weight heparin (LMWH).					
36860098	5	27	theme	weight	924:929	arg1	heparin					931:937	low molecular weight heparin	910:937	low molecular weight heparin (LMWH)	910:944	In their subsequent pregnancies, 55 received prophylaxis with acetylsalicylic acid (ASA) only and 73 received ASA plus low molecular weight heparin (LMWH).					
36860098	5	27	theme	weight	924:929	arg1	LMWH					940:943	LMWH	940:943	LMWH	940:943	In their subsequent pregnancies, 55 received prophylaxis with acetylsalicylic acid (ASA) only and 73 received ASA plus low molecular weight heparin (LMWH).					
36860098	0	28	from	complications	28:40	arg1	women					45:49	women	45:49	women with a history of late fetal loss and placental infarction without thrombophilia	45:130	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	9	29	theme	LMWH	1765:1768	arg1	group					1770:1774	the ASA plus LMWH group	1752:1774	group	1770:1774	An absolute risk reduction of 5.31% was observed for the ASA plus LMWH group.					
36860098	0	30	theme	fetal	74:78	arg1	loss					80:83	late fetal loss	69:83	late fetal loss	69:83	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	8	31	theme	composite	1639:1647	arg1	RR					1659:1660	RR	1659:1660	RR	1659:1660	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	8	31	theme	composite	1639:1647	arg1	outcomes					1649:1656	composite outcomes	1639:1656	composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242)	1639:1696	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	7	32	theme	severe	1242:1247	arg1	preeclampsia					1249:1260	early and/or severe preeclampsia	1229:1260	preeclampsia	1249:1260	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks were 6%, 5%, and 4% respectively.					
36860098	7	33	dep	abruption	1218:1226	arg1	>20 weeks					1278:1286	>20 weeks	1278:1286	>20 weeks	1278:1286	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks were 6%, 5%, and 4% respectively.					
36860098	6	34	theme	%	1073:1073	arg1	births					1063:1068	pre-term births	1054:1068	pre-term births (25% <37 weeks, 5.6% <34 weeks)	1054:1100	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	34	theme	%	1073:1073	arg1	<37 weeks					1075:1083	25% <37 weeks	1071:1083	25% <37 weeks	1071:1083	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	0	35	theme	Placenta-mediated	0:16	arg1	complications					28:40	Placenta-mediated pregnancy complications	0:40	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.	0:210	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	35	theme	Placenta-mediated	0:16	arg1	risk					133:136	risk	133:136	risk of recurrence	133:150	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	35	theme	Placenta-mediated	0:16	arg1	efficacy					156:163	efficacy	156:163	efficacy of pharmacological prophylactic interventions	156:209	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	8	36	theme	delivery	1416:1423	arg1	p = 0.045					1463:1471	RR 0.11, 95% CI: 0.01-0.95 p = 0.045	1436:1471	RR 0.11, 95% CI: 0.01-0.95 p = 0.045	1436:1471	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	8	36	theme	delivery	1416:1423	arg1	<34 weeks					1425:1433	delivery <34 weeks	1416:1433	delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045)	1416:1472	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	6	37	dep	<37 weeks	1075:1083	arg1	<34 weeks					1091:1099	5.6% <34 weeks	1086:1099	5.6% <34 weeks	1086:1099	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	8	38	dep	therapy	1364:1370	arg1	LMWH					1382:1385	LMWH	1382:1385	LMWH	1382:1385	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	8	38	dep	therapy	1364:1370	arg1	ASA					1373:1375	ASA	1373:1375	ASA	1373:1375	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	6	39	theme	pre-term	1054:1061	arg1	births					1063:1068	pre-term births	1054:1068	pre-term births (25% <37 weeks, 5.6% <34 weeks)	1054:1100	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	39	theme	pre-term	1054:1061	arg1	<37 weeks					1075:1083	25% <37 weeks	1071:1083	25% <37 weeks	1071:1083	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	11	40	from	substantial	1995:2005	arg1	CONCLUSION					1888:1897	CONCLUSION	1888:1897	CONCLUSION	1888:1897	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	2	41	theme	fetal	437:441	arg1	loss					443:446	late fetal loss	432:446	late fetal loss without thrombophilia	432:468	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	7	42	theme	early	1229:1233	arg1	preeclampsia					1249:1260	early and/or severe preeclampsia	1229:1260	preeclampsia	1249:1260	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks were 6%, 5%, and 4% respectively.					
36860098	3	43	theme	observational	529:541	arg1	2008-2018					550:558	2008-2018	550:558	2008-2018	550:558	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	3	43	theme	observational	529:541	arg1	study					543:547	a 10-year retrospective observational study	505:547	a 10-year retrospective observational study (2008-2018)	505:559	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	7	44	theme	abruption	1218:1226	arg1	%					1294:1294	6%	1293:1294	6%	1293:1294	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks were 6%, 5%, and 4% respectively.					
36860098	7	44	theme	abruption	1218:1226	arg1	prevalence					1194:1203	The prevalence	1190:1203	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks	1190:1286	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks were 6%, 5%, and 4% respectively.					
36860098	2	45	theme	recurrence	275:284	arg1	risk					267:270	the risk	263:270	the risk of recurrence of severe placenta-mediated pregnancy complications	263:336	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	3	46	theme	10-year	507:513	arg1	2008-2018					550:558	2008-2018	550:558	2008-2018	550:558	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	3	46	theme	10-year	507:513	arg1	study					543:547	a 10-year retrospective observational study	505:547	a 10-year retrospective observational study (2008-2018)	505:559	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	6	47	with	births	1063:1068	arg1	%					1141:1141	17%	1139:1141	17%	1139:1141	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	47	with	births	1063:1068	arg1	weight					1123:1128	birth weight <2500 g	1117:1136	birth weight <2500 g (17%)	1117:1142	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	0	48	dep	complications	28:40	arg1	risk					133:136	risk	133:136	risk of recurrence	133:150	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	48	dep	complications	28:40	arg1	complications					28:40	Placenta-mediated pregnancy complications	0:40	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.	0:210	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	48	dep	complications	28:40	arg1	efficacy					156:163	efficacy	156:163	efficacy of pharmacological prophylactic interventions	156:209	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	10	49	dep	CI	1863:1864	arg1	RR					1850:1851	RR	1850:1851	RR	1850:1851	Multivariate analysis confirmed a risk reduction for delivery <34 weeks (RR 0.32, 95% CI 0.16-0.96 p = 0.041).					
36860098	10	50	theme	delivery	1830:1837	arg1	<34 weeks					1839:1847	delivery <34 weeks	1830:1847	delivery <34 weeks	1830:1847	Multivariate analysis confirmed a risk reduction for delivery <34 weeks (RR 0.32, 95% CI 0.16-0.96 p = 0.041).					
36860098	12	51	theme	ASA	2132:2134	arg1	group					2146:2150	the ASA plus LMWH group	2128:2150	group	2146:2150	A reduction of the risk of delivery <34 weeks was detected in the ASA plus LMWH group.					
36860098	3	52	theme	placental	693:701	arg1	infarction					703:712	placental infarction	693:712	placental infarction	693:712	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	6	53	theme	%	1089:1089	arg1	<34 weeks					1091:1099	5.6% <34 weeks	1086:1099	5.6% <34 weeks	1086:1099	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	12	54	theme	LMWH	2141:2144	arg1	group					2146:2150	the ASA plus LMWH group	2128:2150	group	2146:2150	A reduction of the risk of delivery <34 weeks was detected in the ASA plus LMWH group.					
36860098	0	55	theme	prophylactic	184:195	arg1	interventions					197:209	pharmacological prophylactic interventions	168:209	pharmacological prophylactic interventions	168:209	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	56	with	women	45:49	arg1	infarction					99:108	placental infarction	89:108	placental infarction	89:108	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	56	with	women	45:49	arg1	history					58:64	a history	56:64	a history of late fetal loss	56:83	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	2	57	theme	regimens	396:403	arg1	efficacy					354:361	the efficacy	350:361	the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia	350:468	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	8	58	theme	early/severe	1508:1519	arg1	preeclampsia					1521:1532	early/severe preeclampsia	1508:1532	early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715)	1508:1573	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	8	58	theme	early/severe	1508:1519	arg1	RR					1535:1536	RR	1535:1536	RR	1535:1536	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	2	59	theme	pregnancy	314:322	arg1	complications					324:336	severe placenta-mediated pregnancy complications	289:336	severe placenta-mediated pregnancy complications	289:336	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	2	60	theme	different	370:378	arg1	regimens					396:403	two different anti-thrombotic regimens	366:403	two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia	366:468	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	2	61	theme	severe	289:294	arg1	complications					324:336	severe placenta-mediated pregnancy complications	289:336	severe placenta-mediated pregnancy complications	289:336	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	6	62	with	newborns	1149:1156	arg1	%					1141:1141	17%	1139:1141	17%	1139:1141	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	62	with	newborns	1149:1156	arg1	weight					1123:1128	birth weight <2500 g	1117:1136	birth weight <2500 g (17%)	1117:1142	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	2	63	from	regimens	396:403	arg1	women					408:412	women	408:412	women with a history of late fetal loss without thrombophilia	408:468	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	2	64	from	women	408:412	arg1	efficacy					354:361	the efficacy	350:361	the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia	350:468	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	9	65	theme	risk	1711:1714	arg1	reduction					1716:1724	An absolute risk reduction	1699:1724	An absolute risk reduction of 5.31%	1699:1733	An absolute risk reduction of 5.31% was observed for the ASA plus LMWH group.					
36860098	3	66	theme	women	587:591	arg1	cohort					573:578	a cohort	571:578	a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction	571:712	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	6	67	theme	pregnancies	981:991	arg1	one-third					964:972	one-third	964:972	one-third	964:972	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	67	theme	pregnancies	981:991	arg1	pregnancies					981:991	all pregnancies	977:991	all pregnancies (31%)	977:997	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	67	theme	pregnancies	981:991	arg1	%					996:996	31%	994:996	31%	994:996	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	8	68	theme	risk	1333:1336	arg1	reduction					1338:1346	a risk reduction	1331:1346	a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045)	1331:1472	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	6	69	theme	birth	1117:1121	arg1	%					1141:1141	17%	1139:1141	17%	1139:1141	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	69	theme	birth	1117:1121	arg1	weight					1123:1128	birth weight <2500 g	1117:1136	birth weight <2500 g (17%)	1117:1142	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	11	70	theme	maternal	2031:2038	arg1	conditions					2054:2063	maternal thrombophilic conditions	2031:2063	maternal thrombophilic conditions	2031:2063	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	11	71	theme	conditions	2054:2063	arg1	substantial					1995:2005	substantial	1995:2005	substantial	1995:2005	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	11	71	theme	conditions	2054:2063	arg1	risk					1928:1931	the risk	1924:1931	the risk of recurrence of placenta-mediated pregnancy complications	1924:1990	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	1	72	theme	retrospective	222:234	arg1	study					236:240	A 10-year retrospective study	212:240	A 10-year retrospective study.	212:241	A 10-year retrospective study.					
36860098	10	73	dep	reduction	1816:1824	arg1	CI					1863:1864	CI	1863:1864	CI	1863:1864	Multivariate analysis confirmed a risk reduction for delivery <34 weeks (RR 0.32, 95% CI 0.16-0.96 p = 0.041).					
36860098	8	74	dep	RR	1535:1536	arg1	%					1546:1546	95% CI: 0.01-1.18, p = 0.0715	1544:1572	%	1546:1546	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	11	75	dep	CONCLUSION	1888:1897	arg1	population					1912:1921	our study population	1902:1921	our study population	1902:1921	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	3	76	theme	pregnancy	611:619	arg1	loss					627:630	pregnancy fetal loss	611:630	pregnancy fetal loss	611:630	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	6	77	theme	gestational	1168:1178	arg1	age					1180:1182	gestational age	1168:1182	gestational age (5%)	1168:1187	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	77	theme	gestational	1168:1178	arg1	%					1186:1186	5%	1185:1186	5%	1185:1186	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	3	78	theme	gestational	646:656	arg1	age					658:660	gestational age	646:660	gestational age	646:660	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	6	79	theme	placental	1031:1039	arg1	dysfunction					1041:1051	placental dysfunction	1031:1051	placental dysfunction	1031:1051	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	6	80	theme	small	1158:1162	arg1	newborns					1149:1156	newborns	1149:1156	newborns small for gestational age (5%)	1149:1187	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	4	81	theme	congenital	749:758	arg1	thrombophilia					776:788	congenital and/or acquired thrombophilia	749:788	congenital and/or acquired thrombophilia	749:788	All the women tested negative for congenital and/or acquired thrombophilia.					
36860098	10	82	theme	Multivariate	1777:1788	arg1	analysis					1790:1797	Multivariate analysis	1777:1797	Multivariate analysis	1777:1797	Multivariate analysis confirmed a risk reduction for delivery <34 weeks (RR 0.32, 95% CI 0.16-0.96 p = 0.041).					
36860098	0	83	theme	late	69:72	arg1	loss					80:83	late fetal loss	69:83	late fetal loss	69:83	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	5	84	theme	molecular	914:922	arg1	heparin					931:937	low molecular weight heparin	910:937	low molecular weight heparin (LMWH)	910:944	In their subsequent pregnancies, 55 received prophylaxis with acetylsalicylic acid (ASA) only and 73 received ASA plus low molecular weight heparin (LMWH).					
36860098	5	84	theme	molecular	914:922	arg1	LMWH					940:943	LMWH	940:943	LMWH	940:943	In their subsequent pregnancies, 55 received prophylaxis with acetylsalicylic acid (ASA) only and 73 received ASA plus low molecular weight heparin (LMWH).					
36860098	6	85	theme	related	1020:1026	arg1	outcomes					1011:1018	adverse outcomes	1003:1018	adverse outcomes related to placental dysfunction	1003:1051	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	11	86	theme	complications	1978:1990	arg1	recurrence					1936:1945	recurrence	1936:1945	recurrence of placenta-mediated pregnancy complications	1936:1990	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	0	87	theme	loss	80:83	arg1	infarction					99:108	placental infarction	89:108	placental infarction	89:108	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	87	theme	loss	80:83	arg1	history					58:64	a history	56:64	a history of late fetal loss	56:83	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	8	88	theme	significant	1599:1609	arg1	difference					1611:1620	no statistically significant difference	1582:1620	no statistically significant difference	1582:1620	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	3	89	dep	suffered	597:604	arg1	>20 weeks					633:641	>20 weeks	633:641	>20 weeks of gestational age	633:660	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	6	90	theme	adverse	1003:1009	arg1	outcomes					1011:1018	adverse outcomes	1003:1018	adverse outcomes related to placental dysfunction	1003:1051	RESULTS Overall, one-third of all pregnancies (31%) had adverse outcomes related to placental dysfunction: pre-term births (25% <37 weeks, 5.6% <34 weeks), newborns with birth weight <2500 g (17%), and newborns small for gestational age (5%).					
36860098	11	91	theme	placenta-mediated	1950:1966	arg1	complications					1978:1990	placenta-mediated pregnancy complications	1950:1990	placenta-mediated pregnancy complications	1950:1990	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	2	92	with	women	408:412	arg1	history					421:427	a history	419:427	a history of late fetal loss without thrombophilia	419:468	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	10	93	theme	risk	1811:1814	arg1	reduction					1816:1824	a risk reduction	1809:1824	a risk reduction for delivery <34 weeks (RR 0.32, 95% CI 0.16-0.96 p = 0.041)	1809:1885	Multivariate analysis confirmed a risk reduction for delivery <34 weeks (RR 0.32, 95% CI 0.16-0.96 p = 0.041).					
36860098	0	94	theme	placental	89:97	arg1	infarction					99:108	placental infarction	89:108	placental infarction	89:108	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	5	95	theme	acetylsalicylic	853:867	arg1	acid					869:872	acetylsalicylic acid	853:872	acetylsalicylic acid (ASA)	853:878	In their subsequent pregnancies, 55 received prophylaxis with acetylsalicylic acid (ASA) only and 73 received ASA plus low molecular weight heparin (LMWH).					
36860098	5	95	theme	acetylsalicylic	853:867	arg1	ASA					875:877	ASA	875:877	ASA	875:877	In their subsequent pregnancies, 55 received prophylaxis with acetylsalicylic acid (ASA) only and 73 received ASA plus low molecular weight heparin (LMWH).					
36860098	7	96	theme	preeclampsia	1249:1260	arg1	%					1294:1294	6%	1293:1294	6%	1293:1294	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks were 6%, 5%, and 4% respectively.					
36860098	7	96	theme	preeclampsia	1249:1260	arg1	prevalence					1194:1203	The prevalence	1190:1203	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks	1190:1286	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks were 6%, 5%, and 4% respectively.					
36860098	4	97	theme	acquired	767:774	arg1	thrombophilia					776:788	congenital and/or acquired thrombophilia	749:788	congenital and/or acquired thrombophilia	749:788	All the women tested negative for congenital and/or acquired thrombophilia.					
36860098	11	98	theme	study	1906:1910	arg1	population					1912:1921	our study population	1902:1921	our study population	1902:1921	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	8	99	theme	RR	1436:1437	arg1	%					1447:1447	RR 0.11, 95% CI: 0.01-0.95	1436:1461	%	1447:1447	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	3	100	theme	histological	668:679	arg1	evidence					681:688	histological evidence	668:688	histological evidence of placental infarction	668:712	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	2	101	theme	loss	443:446	arg1	history					421:427	a history	419:427	a history of late fetal loss without thrombophilia	419:468	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	11	102	theme	recurrence	1936:1945	arg1	risk					1928:1931	the risk	1924:1931	the risk of recurrence of placenta-mediated pregnancy complications	1924:1990	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	11	102	theme	recurrence	1936:1945	arg1	substantial					1995:2005	substantial	1995:2005	substantial	1995:2005	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	2	103	theme	late	432:435	arg1	loss					443:446	late fetal loss	432:446	late fetal loss without thrombophilia	432:468	PURPOSE To evaluate the risk of recurrence of severe placenta-mediated pregnancy complications and compare the efficacy of two different anti-thrombotic regimens in women with a history of late fetal loss without thrombophilia.					
36860098	3	104	dep	PATIENTS	471:478	arg1	We					492:493	We	492:493	We	492:493	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	7	105	theme	placental	1208:1216	arg1	abruption					1218:1226	placental abruption	1208:1226	placental abruption	1208:1226	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks were 6%, 5%, and 4% respectively.					
36860098	8	106	theme	%	1447:1447	arg1	p = 0.045					1463:1471	RR 0.11, 95% CI: 0.01-0.95 p = 0.045	1436:1471	RR 0.11, 95% CI: 0.01-0.95 p = 0.045	1436:1471	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	8	106	theme	%	1447:1447	arg1	<34 weeks					1425:1433	delivery <34 weeks	1416:1433	delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045)	1416:1472	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36860098	3	107	theme	infarction	703:712	arg1	evidence					681:688	histological evidence	668:688	histological evidence of placental infarction	668:712	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	12	108	theme	<34 weeks	2102:2110	arg1	risk					2085:2088	the risk	2081:2088	the risk of delivery <34 weeks	2081:2110	A reduction of the risk of delivery <34 weeks was detected in the ASA plus LMWH group.					
36860098	9	109	theme	%	1733:1733	arg1	reduction					1716:1724	An absolute risk reduction	1699:1724	An absolute risk reduction of 5.31%	1699:1733	An absolute risk reduction of 5.31% was observed for the ASA plus LMWH group.					
36860098	3	110	theme	retrospective	515:527	arg1	2008-2018					550:558	2008-2018	550:558	2008-2018	550:558	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	3	110	theme	retrospective	515:527	arg1	study					543:547	a 10-year retrospective observational study	505:547	a 10-year retrospective observational study (2008-2018)	505:559	PATIENTS AND METHODS We performed a 10-year retrospective observational study (2008-2018) analyzing a cohort of 128 women who suffered from pregnancy fetal loss (>20 weeks of gestational age) with histological evidence of placental infarction.					
36860098	7	111	theme	fetal	1267:1271	arg1	loss					1273:1276	fetal loss	1267:1276	fetal loss	1267:1276	The prevalence of placental abruption, early and/or severe preeclampsia, and fetal loss >20 weeks were 6%, 5%, and 4% respectively.					
36860098	11	112	dep	conditions	2054:2063	arg1	absence					2020:2026	absence	2020:2026	absence	2020:2026	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	11	112	dep	conditions	2054:2063	arg1	the					2016:2018	the	2016:2018	the	2016:2018	CONCLUSION In our study population, the risk of recurrence of placenta-mediated pregnancy complications is substantial, even in the absence of maternal thrombophilic conditions.					
36860098	0	113	theme	pregnancy	18:26	arg1	complications					28:40	Placenta-mediated pregnancy complications	0:40	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.	0:210	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	113	theme	pregnancy	18:26	arg1	risk					133:136	risk	133:136	risk of recurrence	133:150	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	0	113	theme	pregnancy	18:26	arg1	efficacy					156:163	efficacy	156:163	efficacy of pharmacological prophylactic interventions	156:209	Placenta-mediated pregnancy complications in women with a history of late fetal loss and placental infarction without thrombophilia: risk of recurrence and efficacy of pharmacological prophylactic interventions.					
36860098	8	114	theme	preeclampsia	1521:1532	arg1	prevention					1494:1503	the prevention	1490:1503	the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715)	1490:1573	We found a risk reduction for combination therapy (ASA plus LMWH) compared with ASA alone for delivery <34 weeks (RR 0.11, 95% CI: 0.01-0.95 p = 0.045) and a trend for the prevention of early/severe preeclampsia (RR 0.14, 95% CI: 0.01-1.18, p = 0.0715), while no statistically significant difference was observed for composite outcomes (RR 0.51, 95%CI: 0.22-1.19, p = 0.1242).					
36543083	0	0	theme	negative	82:89	arg1	therapy					106:112	simulated negative pressure wound therapy	72:112	simulated negative pressure wound therapy	72:112	Influence of advanced wound matrices on observed vacuum pressure during simulated negative pressure wound therapy.					
36543083	6	1	theme	wound	1290:1294	arg1	bed					1296:1298	the wound bed	1286:1298	the wound bed	1286:1298	The reduced performance of the reconstituted collagen products is thought to result from the gelling properties of these products that may cause occlusion of the delivered vacuum to the wound bed.					
36543083	4	2	theme	%	704:704	arg1	reduction					706:714	an ∼50% reduction	698:714	an ∼50% reduction in the observed target vacuum pressure at 75 mmHg and 125 mmHg	698:777	OFM resulted in an ∼50% reduction in the observed target vacuum pressure at 75 mmHg and 125 mmHg, however, this effect was mitigated to a ∼0% reduction when fenestrations were introduced into the matrix.					
36543083	2	3	theme	observed	379:386	arg1	pressure					395:402	the observed vacuum pressure	375:402	the observed vacuum pressure delivered under NPWT	375:423	This study evaluated the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT using a custom test apparatus.					
36543083	0	4	theme	simulated	72:80	arg1	therapy					106:112	simulated negative pressure wound therapy	72:112	simulated negative pressure wound therapy	72:112	Influence of advanced wound matrices on observed vacuum pressure during simulated negative pressure wound therapy.					
36543083	6	5	theme	gelling	1197:1203	arg1	properties					1205:1214	the gelling properties	1193:1214	the gelling properties of these products that may cause occlusion of the delivered vacuum to the wound bed	1193:1298	The reduced performance of the reconstituted collagen products is thought to result from the gelling properties of these products that may cause occlusion of the delivered vacuum to the wound bed.					
36543083	3	6	theme	forestomach	532:542	arg1	products					516:523	the commercial products	501:523	the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD)	501:647	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	6	theme	forestomach	532:542	arg1	OFM					552:554	OFM	552:554	OFM	552:554	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	6	theme	forestomach	532:542	arg1	matrix					544:549	ovine forestomach matrix	526:549	ovine forestomach matrix (OFM)	526:555	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	2	7	theme	collagen-based	348:361	arg1	matrices					363:370	commercially available collagen-based matrices	325:370	commercially available collagen-based matrices	325:370	This study evaluated the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT using a custom test apparatus.					
36543083	0	8	theme	wound	100:104	arg1	therapy					106:112	simulated negative pressure wound therapy	72:112	simulated negative pressure wound therapy	72:112	Influence of advanced wound matrices on observed vacuum pressure during simulated negative pressure wound therapy.					
36543083	0	9	from	Influence	0:8	arg1	pressure					56:63	observed vacuum pressure	40:63	observed vacuum pressure during simulated negative pressure wound therapy	40:112	Influence of advanced wound matrices on observed vacuum pressure during simulated negative pressure wound therapy.					
36543083	4	10	from	125 mmHg	770:777	arg1	reduction					706:714	an ∼50% reduction	698:714	an ∼50% reduction in the observed target vacuum pressure at 75 mmHg and 125 mmHg	698:777	OFM resulted in an ∼50% reduction in the observed target vacuum pressure at 75 mmHg and 125 mmHg, however, this effect was mitigated to a ∼0% reduction when fenestrations were introduced into the matrix.					
36543083	2	11	theme	design	299:304	arg1	impact					285:290	the impact	281:290	the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT	281:423	This study evaluated the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT using a custom test apparatus.					
36543083	7	12	theme	pressure	1453:1460	arg1	delivery					1434:1441	effective delivery	1424:1441	effective delivery of vacuum pressure	1424:1460	These findings highlight the importance of in vitro testing to establish the impact of adjunctive therapies on NPWT, where effective delivery of vacuum pressure is paramount to the efficacy of this therapy.					
36543083	0	13	theme	pressure	91:98	arg1	therapy					106:112	simulated negative pressure wound therapy	72:112	simulated negative pressure wound therapy	72:112	Influence of advanced wound matrices on observed vacuum pressure during simulated negative pressure wound therapy.					
36543083	5	14	theme	vacuum	1032:1037	arg1	pressure					1039:1046	a lower vacuum pressure	1024:1046	a lower vacuum pressure of 75 mmHg	1024:1057	Both collagen/ORC and CWD reduced the observed vacuum pressure at 125 mmHg (∼15% and ∼50%, respectively), and this was more dramatic when a lower vacuum pressure of 75 mmHg was delivered (∼20% and ∼75%, respectively).					
36543083	3	15	theme	collagen/oxidized	558:574	arg1	products					516:523	the commercial products	501:523	the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD)	501:647	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	15	theme	collagen/oxidized	558:574	arg1	cellulose					588:596	collagen/oxidized regenerated cellulose	558:596	collagen/oxidized regenerated cellulose (collagen/ORC)	558:611	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	15	theme	collagen/oxidized	558:574	arg1	collagen/ORC					599:610	collagen/ORC	599:610	collagen/ORC	599:610	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	2	16	dep	design	299:304	arg1	the					295:297	the	295:297	the	295:297	This study evaluated the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT using a custom test apparatus.					
36543083	2	17	theme	available	338:346	arg1	matrices					363:370	commercially available collagen-based matrices	325:370	commercially available collagen-based matrices	325:370	This study evaluated the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT using a custom test apparatus.					
36543083	6	18	theme	delivered	1266:1274	arg1	vacuum					1276:1281	the delivered vacuum	1262:1281	the delivered vacuum	1262:1281	The reduced performance of the reconstituted collagen products is thought to result from the gelling properties of these products that may cause occlusion of the delivered vacuum to the wound bed.					
36543083	4	19	theme	%	822:822	arg1	reduction					824:832	a ∼0% reduction	818:832	a ∼0% reduction when fenestrations were introduced into the matrix	818:883	OFM resulted in an ∼50% reduction in the observed target vacuum pressure at 75 mmHg and 125 mmHg, however, this effect was mitigated to a ∼0% reduction when fenestrations were introduced into the matrix.					
36543083	4	20	from	75 mmHg	758:764	arg1	reduction					706:714	an ∼50% reduction	698:714	an ∼50% reduction in the observed target vacuum pressure at 75 mmHg and 125 mmHg	698:777	OFM resulted in an ∼50% reduction in the observed target vacuum pressure at 75 mmHg and 125 mmHg, however, this effect was mitigated to a ∼0% reduction when fenestrations were introduced into the matrix.					
36543083	3	21	dep	products	516:523	arg1	matrix					544:549	ovine forestomach matrix	526:549	ovine forestomach matrix (OFM)	526:555	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	21	dep	products	516:523	arg1	cellulose					588:596	collagen/oxidized regenerated cellulose	558:596	collagen/oxidized regenerated cellulose (collagen/ORC)	558:611	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	21	dep	products	516:523	arg1	dressing					634:641	a collagen-based dressing	617:641	a collagen-based dressing (CWD)	617:647	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	21	dep	products	516:523	arg1	collagen/ORC					599:610	collagen/ORC	599:610	collagen/ORC	599:610	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	21	dep	products	516:523	arg1	products					516:523	the commercial products	501:523	the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD)	501:647	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	21	dep	products	516:523	arg1	OFM					552:554	OFM	552:554	OFM	552:554	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	21	dep	products	516:523	arg1	CWD					644:646	CWD	644:646	CWD	644:646	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	7	22	theme	testing	1353:1359	arg1	importance					1330:1339	the importance	1326:1339	the importance of in vitro testing to establish the impact of adjunctive therapies on NPWT, where effective delivery of vacuum pressure is paramount to the efficacy of this therapy	1326:1505	These findings highlight the importance of in vitro testing to establish the impact of adjunctive therapies on NPWT, where effective delivery of vacuum pressure is paramount to the efficacy of this therapy.					
36543083	7	23	from	impact	1378:1383	arg1	NPWT					1412:1415	NPWT	1412:1415	NPWT	1412:1415	These findings highlight the importance of in vitro testing to establish the impact of adjunctive therapies on NPWT, where effective delivery of vacuum pressure is paramount to the efficacy of this therapy.					
36543083	7	24	theme	therapy	1499:1505	arg1	efficacy					1482:1489	the efficacy	1478:1489	the efficacy of this therapy	1478:1505	These findings highlight the importance of in vitro testing to establish the impact of adjunctive therapies on NPWT, where effective delivery of vacuum pressure is paramount to the efficacy of this therapy.					
36543083	5	25	theme	75 mmHg	1051:1057	arg1	pressure					1039:1046	a lower vacuum pressure	1024:1046	a lower vacuum pressure of 75 mmHg	1024:1057	Both collagen/ORC and CWD reduced the observed vacuum pressure at 125 mmHg (∼15% and ∼50%, respectively), and this was more dramatic when a lower vacuum pressure of 75 mmHg was delivered (∼20% and ∼75%, respectively).					
36543083	4	26	theme	vacuum	739:744	arg1	pressure					746:753	the observed target vacuum pressure	719:753	the observed target vacuum pressure	719:753	OFM resulted in an ∼50% reduction in the observed target vacuum pressure at 75 mmHg and 125 mmHg, however, this effect was mitigated to a ∼0% reduction when fenestrations were introduced into the matrix.					
36543083	1	27	theme	treatment	175:183	arg1	modalities					185:194	treatment modalities	175:194	treatment modalities	175:194	Biomaterials and negative pressure wound therapy (NPWT) are treatment modalities regularly used together to accelerate soft-tissue regeneration.					
36543083	7	28	theme	in	1344:1345	arg1	testing					1353:1359	in vitro testing	1344:1359	in vitro testing	1344:1359	These findings highlight the importance of in vitro testing to establish the impact of adjunctive therapies on NPWT, where effective delivery of vacuum pressure is paramount to the efficacy of this therapy.					
36543083	7	29	theme	therapies	1399:1407	arg1	impact					1378:1383	the impact	1374:1383	the impact of adjunctive therapies on NPWT, where effective delivery of vacuum pressure is paramount to the efficacy of this therapy	1374:1505	These findings highlight the importance of in vitro testing to establish the impact of adjunctive therapies on NPWT, where effective delivery of vacuum pressure is paramount to the efficacy of this therapy.					
36543083	4	30	theme	target	732:737	arg1	pressure					746:753	the observed target vacuum pressure	719:753	the observed target vacuum pressure	719:753	OFM resulted in an ∼50% reduction in the observed target vacuum pressure at 75 mmHg and 125 mmHg, however, this effect was mitigated to a ∼0% reduction when fenestrations were introduced into the matrix.					
36543083	7	31	theme	vacuum	1446:1451	arg1	pressure					1453:1460	vacuum pressure	1446:1460	vacuum pressure	1446:1460	These findings highlight the importance of in vitro testing to establish the impact of adjunctive therapies on NPWT, where effective delivery of vacuum pressure is paramount to the efficacy of this therapy.					
36543083	0	32	theme	wound	22:26	arg1	matrices					28:35	advanced wound matrices	13:35	advanced wound matrices	13:35	Influence of advanced wound matrices on observed vacuum pressure during simulated negative pressure wound therapy.					
36543083	2	33	theme	composition	310:320	arg1	impact					285:290	the impact	281:290	the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT	281:423	This study evaluated the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT using a custom test apparatus.					
36543083	0	34	theme	advanced	13:20	arg1	matrices					28:35	advanced wound matrices	13:35	advanced wound matrices	13:35	Influence of advanced wound matrices on observed vacuum pressure during simulated negative pressure wound therapy.					
36543083	5	35	theme	observed	924:931	arg1	pressure					940:947	the observed vacuum pressure	920:947	the observed vacuum pressure	920:947	Both collagen/ORC and CWD reduced the observed vacuum pressure at 125 mmHg (∼15% and ∼50%, respectively), and this was more dramatic when a lower vacuum pressure of 75 mmHg was delivered (∼20% and ∼75%, respectively).					
36543083	3	36	theme	observed	656:663	arg1	pressure					672:679	the observed vacuum pressure	652:679	the observed vacuum pressure	652:679	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	2	37	theme	test	440:443	arg1	apparatus					445:453	a custom test apparatus	431:453	a custom test apparatus	431:453	This study evaluated the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT using a custom test apparatus.					
36543083	3	38	theme	ovine	526:530	arg1	products					516:523	the commercial products	501:523	the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD)	501:647	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	38	theme	ovine	526:530	arg1	OFM					552:554	OFM	552:554	OFM	552:554	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	38	theme	ovine	526:530	arg1	matrix					544:549	ovine forestomach matrix	526:549	ovine forestomach matrix (OFM)	526:555	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	5	39	dep	delivered	1063:1071	arg1	%					1077:1077	∼20%	1074:1077	∼20%	1074:1077	Both collagen/ORC and CWD reduced the observed vacuum pressure at 125 mmHg (∼15% and ∼50%, respectively), and this was more dramatic when a lower vacuum pressure of 75 mmHg was delivered (∼20% and ∼75%, respectively).					
36543083	5	39	dep	delivered	1063:1071	arg1	%					1086:1086	∼75%	1083:1086	∼75%	1083:1086	Both collagen/ORC and CWD reduced the observed vacuum pressure at 125 mmHg (∼15% and ∼50%, respectively), and this was more dramatic when a lower vacuum pressure of 75 mmHg was delivered (∼20% and ∼75%, respectively).					
36543083	6	40	theme	reconstituted	1135:1147	arg1	products					1158:1165	the reconstituted collagen products	1131:1165	the reconstituted collagen products	1131:1165	The reduced performance of the reconstituted collagen products is thought to result from the gelling properties of these products that may cause occlusion of the delivered vacuum to the wound bed.					
36543083	3	41	theme	vacuum	665:670	arg1	pressure					672:679	the observed vacuum pressure	652:679	the observed vacuum pressure	652:679	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	6	42	theme	products	1158:1165	arg1	performance					1116:1126	The reduced performance	1104:1126	The reduced performance of the reconstituted collagen products	1104:1165	The reduced performance of the reconstituted collagen products is thought to result from the gelling properties of these products that may cause occlusion of the delivered vacuum to the wound bed.					
36543083	4	43	from	reduction	706:714	arg1	pressure					746:753	the observed target vacuum pressure	719:753	the observed target vacuum pressure	719:753	OFM resulted in an ∼50% reduction in the observed target vacuum pressure at 75 mmHg and 125 mmHg, however, this effect was mitigated to a ∼0% reduction when fenestrations were introduced into the matrix.					
36543083	2	44	theme	custom	433:438	arg1	test					440:443	a custom test	431:443	a custom test apparatus	431:453	This study evaluated the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT using a custom test apparatus.					
36543083	0	45	theme	matrices	28:35	arg1	Influence					0:8	Influence	0:8	Influence of advanced wound matrices on observed vacuum pressure during simulated negative pressure wound therapy.	0:113	Influence of advanced wound matrices on observed vacuum pressure during simulated negative pressure wound therapy.					
36543083	7	46	theme	effective	1424:1432	arg1	delivery					1434:1441	effective delivery	1424:1441	effective delivery of vacuum pressure	1424:1460	These findings highlight the importance of in vitro testing to establish the impact of adjunctive therapies on NPWT, where effective delivery of vacuum pressure is paramount to the efficacy of this therapy.					
36543083	3	47	theme	collagen-based	619:632	arg1	products					516:523	the commercial products	501:523	the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD)	501:647	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	47	theme	collagen-based	619:632	arg1	CWD					644:646	CWD	644:646	CWD	644:646	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	47	theme	collagen-based	619:632	arg1	dressing					634:641	a collagen-based dressing	617:641	a collagen-based dressing (CWD)	617:647	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	7	48	dep	in	1344:1345	arg1	vitro					1347:1351	vitro	1347:1351	vitro	1347:1351	These findings highlight the importance of in vitro testing to establish the impact of adjunctive therapies on NPWT, where effective delivery of vacuum pressure is paramount to the efficacy of this therapy.					
36543083	0	49	theme	vacuum	49:54	arg1	pressure					56:63	observed vacuum pressure	40:63	observed vacuum pressure during simulated negative pressure wound therapy	40:112	Influence of advanced wound matrices on observed vacuum pressure during simulated negative pressure wound therapy.					
36543083	5	50	theme	lower	1026:1030	arg1	pressure					1039:1046	a lower vacuum pressure	1024:1046	a lower vacuum pressure of 75 mmHg	1024:1057	Both collagen/ORC and CWD reduced the observed vacuum pressure at 125 mmHg (∼15% and ∼50%, respectively), and this was more dramatic when a lower vacuum pressure of 75 mmHg was delivered (∼20% and ∼75%, respectively).					
36543083	1	51	used	used	206:209	arg2	NPWT					165:168	NPWT	165:168	NPWT	165:168	Biomaterials and negative pressure wound therapy (NPWT) are treatment modalities regularly used together to accelerate soft-tissue regeneration.					
36543083	1	51	used	used	206:209	arg2	Biomaterials					115:126	Biomaterials	115:126	Biomaterials	115:126	Biomaterials and negative pressure wound therapy (NPWT) are treatment modalities regularly used together to accelerate soft-tissue regeneration.					
36543083	1	51	used	used	206:209	arg2	therapy					156:162	negative pressure wound therapy	132:162	negative pressure wound therapy (NPWT)	132:169	Biomaterials and negative pressure wound therapy (NPWT) are treatment modalities regularly used together to accelerate soft-tissue regeneration.					
36543083	3	52	theme	products	516:523	arg1	effect					491:496	the effect	487:496	the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure	487:679	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	0	53	theme	observed	40:47	arg1	pressure					56:63	observed vacuum pressure	40:63	observed vacuum pressure during simulated negative pressure wound therapy	40:112	Influence of advanced wound matrices on observed vacuum pressure during simulated negative pressure wound therapy.					
36543083	3	54	theme	regenerated	576:586	arg1	products					516:523	the commercial products	501:523	the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD)	501:647	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	54	theme	regenerated	576:586	arg1	cellulose					588:596	collagen/oxidized regenerated cellulose	558:596	collagen/oxidized regenerated cellulose (collagen/ORC)	558:611	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	54	theme	regenerated	576:586	arg1	collagen/ORC					599:610	collagen/ORC	599:610	collagen/ORC	599:610	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	6	55	theme	products	1225:1232	arg1	properties					1205:1214	the gelling properties	1193:1214	the gelling properties of these products that may cause occlusion of the delivered vacuum to the wound bed	1193:1298	The reduced performance of the reconstituted collagen products is thought to result from the gelling properties of these products that may cause occlusion of the delivered vacuum to the wound bed.					
36543083	3	56	from	effect	491:496	arg1	pressure					672:679	the observed vacuum pressure	652:679	the observed vacuum pressure	652:679	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	2	57	theme	matrices	363:370	arg1	composition					310:320	composition	310:320	composition	310:320	This study evaluated the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT using a custom test apparatus.					
36543083	2	57	theme	matrices	363:370	arg1	design					299:304	design	299:304	design	299:304	This study evaluated the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT using a custom test apparatus.					
36543083	5	58	theme	vacuum	933:938	arg1	pressure					940:947	the observed vacuum pressure	920:947	the observed vacuum pressure	920:947	Both collagen/ORC and CWD reduced the observed vacuum pressure at 125 mmHg (∼15% and ∼50%, respectively), and this was more dramatic when a lower vacuum pressure of 75 mmHg was delivered (∼20% and ∼75%, respectively).					
36543083	6	59	theme	collagen	1149:1156	arg1	products					1158:1165	the reconstituted collagen products	1131:1165	the reconstituted collagen products	1131:1165	The reduced performance of the reconstituted collagen products is thought to result from the gelling properties of these products that may cause occlusion of the delivered vacuum to the wound bed.					
36543083	1	60	theme	negative	132:139	arg1	therapy					156:162	negative pressure wound therapy	132:162	negative pressure wound therapy (NPWT)	132:169	Biomaterials and negative pressure wound therapy (NPWT) are treatment modalities regularly used together to accelerate soft-tissue regeneration.					
36543083	1	60	theme	negative	132:139	arg1	NPWT					165:168	NPWT	165:168	NPWT	165:168	Biomaterials and negative pressure wound therapy (NPWT) are treatment modalities regularly used together to accelerate soft-tissue regeneration.					
36543083	2	61	from	impact	285:290	arg1	pressure					395:402	the observed vacuum pressure	375:402	the observed vacuum pressure delivered under NPWT	375:423	This study evaluated the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT using a custom test apparatus.					
36543083	4	62	theme	observed	723:730	arg1	pressure					746:753	the observed target vacuum pressure	719:753	the observed target vacuum pressure	719:753	OFM resulted in an ∼50% reduction in the observed target vacuum pressure at 75 mmHg and 125 mmHg, however, this effect was mitigated to a ∼0% reduction when fenestrations were introduced into the matrix.					
36543083	1	63	theme	pressure	141:148	arg1	therapy					156:162	negative pressure wound therapy	132:162	negative pressure wound therapy (NPWT)	132:169	Biomaterials and negative pressure wound therapy (NPWT) are treatment modalities regularly used together to accelerate soft-tissue regeneration.					
36543083	1	63	theme	pressure	141:148	arg1	NPWT					165:168	NPWT	165:168	NPWT	165:168	Biomaterials and negative pressure wound therapy (NPWT) are treatment modalities regularly used together to accelerate soft-tissue regeneration.					
36543083	5	64	dep	reduced	912:918	arg1	%					965:965	∼15%	962:965	∼15%	962:965	Both collagen/ORC and CWD reduced the observed vacuum pressure at 125 mmHg (∼15% and ∼50%, respectively), and this was more dramatic when a lower vacuum pressure of 75 mmHg was delivered (∼20% and ∼75%, respectively).					
36543083	5	64	dep	reduced	912:918	arg1	%					974:974	∼50%	971:974	∼50%	971:974	Both collagen/ORC and CWD reduced the observed vacuum pressure at 125 mmHg (∼15% and ∼50%, respectively), and this was more dramatic when a lower vacuum pressure of 75 mmHg was delivered (∼20% and ∼75%, respectively).					
36543083	1	65	theme	soft-tissue	234:244	arg1	regeneration					246:257	soft-tissue regeneration	234:257	soft-tissue regeneration	234:257	Biomaterials and negative pressure wound therapy (NPWT) are treatment modalities regularly used together to accelerate soft-tissue regeneration.					
36543083	6	66	theme	reduced	1108:1114	arg1	performance					1116:1126	The reduced performance	1104:1126	The reduced performance of the reconstituted collagen products	1104:1165	The reduced performance of the reconstituted collagen products is thought to result from the gelling properties of these products that may cause occlusion of the delivered vacuum to the wound bed.					
36543083	1	67	theme	wound	150:154	arg1	therapy					156:162	negative pressure wound therapy	132:162	negative pressure wound therapy (NPWT)	132:169	Biomaterials and negative pressure wound therapy (NPWT) are treatment modalities regularly used together to accelerate soft-tissue regeneration.					
36543083	1	67	theme	wound	150:154	arg1	NPWT					165:168	NPWT	165:168	NPWT	165:168	Biomaterials and negative pressure wound therapy (NPWT) are treatment modalities regularly used together to accelerate soft-tissue regeneration.					
36543083	3	68	theme	commercial	505:514	arg1	products					516:523	the commercial products	501:523	the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD)	501:647	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	68	theme	commercial	505:514	arg1	cellulose					588:596	collagen/oxidized regenerated cellulose	558:596	collagen/oxidized regenerated cellulose (collagen/ORC)	558:611	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	68	theme	commercial	505:514	arg1	matrix					544:549	ovine forestomach matrix	526:549	ovine forestomach matrix (OFM)	526:555	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	3	68	theme	commercial	505:514	arg1	dressing					634:641	a collagen-based dressing	617:641	a collagen-based dressing (CWD)	617:647	Specifically, testing compared the effect of the commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) and a collagen-based dressing (CWD) on the observed vacuum pressure.					
36543083	7	69	theme	adjunctive	1388:1397	arg1	therapies					1399:1407	adjunctive therapies	1388:1407	adjunctive therapies	1388:1407	These findings highlight the importance of in vitro testing to establish the impact of adjunctive therapies on NPWT, where effective delivery of vacuum pressure is paramount to the efficacy of this therapy.					
36543083	2	70	theme	vacuum	388:393	arg1	pressure					395:402	the observed vacuum pressure	375:402	the observed vacuum pressure delivered under NPWT	375:423	This study evaluated the impact of the design and composition of commercially available collagen-based matrices on the observed vacuum pressure delivered under NPWT using a custom test apparatus.					
36543083	6	71	theme	vacuum	1276:1281	arg1	occlusion					1249:1257	occlusion	1249:1257	occlusion of the delivered vacuum to the wound bed	1249:1298	The reduced performance of the reconstituted collagen products is thought to result from the gelling properties of these products that may cause occlusion of the delivered vacuum to the wound bed.					
35725175	2	0	theme	physical	521:528	arg1	properties					530:539	physical properties	521:539	physical properties	521:539	The morphology, structure, physical properties, antioxidant and antibacterial activities of the films were characterized and analyzed.					
35725175	2	1	dep	morphology	498:507	arg1	The					494:496	The	494:496	The	494:496	The morphology, structure, physical properties, antioxidant and antibacterial activities of the films were characterized and analyzed.					
35725175	1	2	from	effects	158:164	arg1	properties					303:312	the properties	299:312	the properties of sodium alginate (SA) composite films	299:352	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	3	3	theme	barrier	685:691	arg1	property					693:700	light barrier property	679:700	light barrier property	679:700	The results showed that the thermal stability and light barrier property of the films were improved by the addition of IRMOF-3/CA.					
35725175	5	4	theme	application	1135:1145	arg1	potential					1147:1155	its application potential	1131:1155	its application potential in food packaging	1131:1173	Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities, and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.					
35725175	5	5	from	potential	1147:1155	arg1	packaging					1165:1173	food packaging	1160:1173	food packaging	1160:1173	Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities, and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.					
35725175	1	6	theme	antioxidant	470:480	arg1	activities					482:491	antibacterial and antioxidant activities	452:491	antibacterial and antioxidant activities	452:491	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	2	7	theme	antibacterial	558:570	arg1	activities					572:581	antibacterial activities	558:581	antibacterial activities	558:581	The morphology, structure, physical properties, antioxidant and antibacterial activities of the films were characterized and analyzed.					
35725175	5	8	theme	fresh-keeping	1090:1102	arg1	effect					1104:1109	an apparent fresh-keeping effect	1078:1109	an apparent fresh-keeping effect	1078:1109	Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities, and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.					
35725175	4	9	theme	SA	964:965	arg1	film					967:970	pure SA film	959:970	pure SA film	959:970	When the additional concentration was 0.4 wt%, the tensile, water vapor barrier and hydrophobic properties of the films were increased by 30.13%, 9.06% and 46.43% respectively compared with those of pure SA film.					
35725175	1	10	theme	optimal	376:382	arg1	amount					393:398	the optimal addition amount	372:398	the optimal addition amount	372:398	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	4	11	theme	films	874:878	arg1	barrier					832:838	the tensile, water vapor barrier and hydrophobic properties	807:865	barrier	832:838	When the additional concentration was 0.4 wt%, the tensile, water vapor barrier and hydrophobic properties of the films were increased by 30.13%, 9.06% and 46.43% respectively compared with those of pure SA film.					
35725175	4	11	theme	films	874:878	arg1	properties					856:865	the tensile, water vapor barrier and hydrophobic properties	807:865	properties	856:865	When the additional concentration was 0.4 wt%, the tensile, water vapor barrier and hydrophobic properties of the films were increased by 30.13%, 9.06% and 46.43% respectively compared with those of pure SA film.					
35725175	4	12	theme	tensile	811:817	arg1	barrier					832:838	the tensile, water vapor barrier and hydrophobic properties	807:865	barrier	832:838	When the additional concentration was 0.4 wt%, the tensile, water vapor barrier and hydrophobic properties of the films were increased by 30.13%, 9.06% and 46.43% respectively compared with those of pure SA film.					
35725175	1	13	theme	different	169:177	arg1	concentrations					179:192	different concentrations	169:192	different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA)	169:294	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	4	14	theme	pure	959:962	arg1	film					967:970	pure SA film	959:970	pure SA film	959:970	When the additional concentration was 0.4 wt%, the tensile, water vapor barrier and hydrophobic properties of the films were increased by 30.13%, 9.06% and 46.43% respectively compared with those of pure SA film.					
35725175	1	15	theme	addition	384:391	arg1	amount					393:398	the optimal addition amount	372:398	the optimal addition amount	372:398	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	1	16	theme	concentrations	179:192	arg1	effects					158:164	the effects	154:164	the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films	154:352	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	4	17	dep	increased	885:893	arg1	compared					936:943	compared	936:943	compared with those of pure SA film	936:970	When the additional concentration was 0.4 wt%, the tensile, water vapor barrier and hydrophobic properties of the films were increased by 30.13%, 9.06% and 46.43% respectively compared with those of pure SA film.					
35725175	3	18	theme	films	709:713	arg1	property					693:700	light barrier property	679:700	light barrier property	679:700	The results showed that the thermal stability and light barrier property of the films were improved by the addition of IRMOF-3/CA.					
35725175	3	18	theme	films	709:713	arg1	stability					665:673	thermal stability	657:673	thermal stability	657:673	The results showed that the thermal stability and light barrier property of the films were improved by the addition of IRMOF-3/CA.					
35725175	4	19	theme	additional	769:778	arg1	concentration					780:792	the additional concentration	765:792	the additional concentration	765:792	When the additional concentration was 0.4 wt%, the tensile, water vapor barrier and hydrophobic properties of the films were increased by 30.13%, 9.06% and 46.43% respectively compared with those of pure SA film.					
35725175	4	19	theme	additional	769:778	arg1	%					804:804	0.4 wt%	798:804	0.4 wt%	798:804	When the additional concentration was 0.4 wt%, the tensile, water vapor barrier and hydrophobic properties of the films were increased by 30.13%, 9.06% and 46.43% respectively compared with those of pure SA film.					
35725175	0	20	theme	alginate-based	22:35	arg1	antioxidant					37:47	sodium alginate-based antioxidant	15:47	sodium alginate-based antioxidant	15:47	Development of sodium alginate-based antioxidant and antibacterial bioactive films added with IRMOF-3/Carvacrol.					
35725175	5	21	theme	apparent	1081:1088	arg1	effect					1104:1109	an apparent fresh-keeping effect	1078:1109	an apparent fresh-keeping effect	1078:1109	Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities, and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.					
35725175	1	22	dep	compare	146:152	arg1	preparing					412:420	preparing	412:420	further preparing bioactive packaging film with antibacterial and antioxidant activities	404:491	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	1	22	dep	compare	146:152	arg1	determining					360:370	determining	360:370	determining the optimal addition amount	360:398	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	1	23	theme	amine-functionalized	201:220	arg1	framework-3					249:259	the amine-functionalized isoreticular metal-organic framework-3	197:259	the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA)	197:294	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	0	24	theme	sodium	15:20	arg1	antioxidant					37:47	sodium alginate-based antioxidant	15:47	sodium alginate-based antioxidant	15:47	Development of sodium alginate-based antioxidant and antibacterial bioactive films added with IRMOF-3/Carvacrol.					
35725175	2	25	theme	films	590:594	arg1	properties					530:539	physical properties	521:539	physical properties	521:539	The morphology, structure, physical properties, antioxidant and antibacterial activities of the films were characterized and analyzed.					
35725175	2	25	theme	films	590:594	arg1	morphology					498:507	morphology	498:507	morphology	498:507	The morphology, structure, physical properties, antioxidant and antibacterial activities of the films were characterized and analyzed.					
35725175	2	25	theme	films	590:594	arg1	structure					510:518	structure	510:518	structure	510:518	The morphology, structure, physical properties, antioxidant and antibacterial activities of the films were characterized and analyzed.					
35725175	2	25	theme	films	590:594	arg1	antioxidant					542:552	antioxidant	542:552	antioxidant	542:552	The morphology, structure, physical properties, antioxidant and antibacterial activities of the films were characterized and analyzed.					
35725175	2	25	theme	films	590:594	arg1	activities					572:581	antibacterial activities	558:581	antibacterial activities	558:581	The morphology, structure, physical properties, antioxidant and antibacterial activities of the films were characterized and analyzed.					
35725175	1	26	theme	isoreticular	222:233	arg1	framework-3					249:259	the amine-functionalized isoreticular metal-organic framework-3	197:259	the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA)	197:294	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	3	27	dep	stability	665:673	arg1	the					653:655	the	653:655	the	653:655	The results showed that the thermal stability and light barrier property of the films were improved by the addition of IRMOF-3/CA.					
35725175	4	28	theme	hydrophobic	844:854	arg1	properties					856:865	the tensile, water vapor barrier and hydrophobic properties	807:865	properties	856:865	When the additional concentration was 0.4 wt%, the tensile, water vapor barrier and hydrophobic properties of the films were increased by 30.13%, 9.06% and 46.43% respectively compared with those of pure SA film.					
35725175	1	29	theme	metal-organic	235:247	arg1	framework-3					249:259	the amine-functionalized isoreticular metal-organic framework-3	197:259	the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA)	197:294	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	0	30	theme	antioxidant	37:47	arg1	Development					0:10	Development	0:10	Development of sodium alginate-based antioxidant and antibacterial bioactive films	0:81	Development of sodium alginate-based antioxidant and antibacterial bioactive films added with IRMOF-3/Carvacrol.					
35725175	3	31	theme	light	679:683	arg1	property					693:700	light barrier property	679:700	light barrier property	679:700	The results showed that the thermal stability and light barrier property of the films were improved by the addition of IRMOF-3/CA.					
35725175	5	32	contain	had	1074:1076	arg1	film					987:990	the film	983:990	the film added with IRMOF-3/CA	983:1012	Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities, and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.					
35725175	5	32	contain	had	1074:1076	arg2	effect					1104:1109	an apparent fresh-keeping effect	1078:1109	an apparent fresh-keeping effect	1078:1109	Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities, and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.					
35725175	1	33	theme	framework-3	249:259	arg1	concentrations					179:192	different concentrations	169:192	different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA)	169:294	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	0	34	theme	bioactive	67:75	arg1	films					77:81	antibacterial bioactive films	53:81	antibacterial bioactive films	53:81	Development of sodium alginate-based antioxidant and antibacterial bioactive films added with IRMOF-3/Carvacrol.					
35725175	1	35	theme	bioactive	422:430	arg1	film					442:445	bioactive packaging film	422:445	bioactive packaging film	422:445	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	5	36	theme	sustained	1018:1026	arg1	antioxidant					1028:1038	sustained antioxidant	1018:1038	sustained antioxidant	1018:1038	Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities, and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.					
35725175	3	37	theme	IRMOF-3/CA	748:757	arg1	addition					736:743	the addition	732:743	the addition of IRMOF-3/CA	732:757	The results showed that the thermal stability and light barrier property of the films were improved by the addition of IRMOF-3/CA.					
35725175	0	38	theme	antibacterial	53:65	arg1	films					77:81	antibacterial bioactive films	53:81	antibacterial bioactive films	53:81	Development of sodium alginate-based antioxidant and antibacterial bioactive films added with IRMOF-3/Carvacrol.					
35725175	5	39	theme	food	1160:1163	arg1	packaging					1165:1173	food packaging	1160:1173	food packaging	1160:1173	Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities, and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.					
35725175	1	40	theme	packaging	432:440	arg1	film					442:445	bioactive packaging film	422:445	bioactive packaging film	422:445	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	1	41	theme	study	133:137	arg1	purpose					117:123	The purpose	113:123	The purpose of this study	113:137	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	1	42	theme	composite	338:346	arg1	films					348:352	sodium alginate (SA) composite films	317:352	sodium alginate (SA) composite films	317:352	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	5	43	theme	antibacterial	1044:1056	arg1	activities					1058:1067	antibacterial activities	1044:1067	antibacterial activities	1044:1067	Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities, and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.					
35725175	5	44	contain	had	1014:1016	arg2	antioxidant					1028:1038	sustained antioxidant	1018:1038	sustained antioxidant	1018:1038	Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities, and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.					
35725175	5	44	contain	had	1014:1016	arg1	film					987:990	the film	983:990	the film added with IRMOF-3/CA	983:1012	Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities, and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.					
35725175	5	44	contain	had	1014:1016	arg2	activities					1058:1067	antibacterial activities	1044:1067	antibacterial activities	1044:1067	Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities, and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.					
35725175	1	45	theme	antibacterial	452:464	arg1	activities					482:491	antibacterial and antioxidant activities	452:491	antibacterial and antioxidant activities	452:491	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	4	46	dep	tensile	811:817	arg1	vapor					826:830	vapor	826:830	vapor	826:830	When the additional concentration was 0.4 wt%, the tensile, water vapor barrier and hydrophobic properties of the films were increased by 30.13%, 9.06% and 46.43% respectively compared with those of pure SA film.					
35725175	0	47	theme	films	77:81	arg1	Development					0:10	Development	0:10	Development of sodium alginate-based antioxidant and antibacterial bioactive films	0:81	Development of sodium alginate-based antioxidant and antibacterial bioactive films added with IRMOF-3/Carvacrol.					
35725175	1	48	theme	films	348:352	arg1	properties					303:312	the properties	299:312	the properties of sodium alginate (SA) composite films	299:352	The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities.					
35725175	3	49	theme	thermal	657:663	arg1	stability					665:673	thermal stability	657:673	thermal stability	657:673	The results showed that the thermal stability and light barrier property of the films were improved by the addition of IRMOF-3/CA.					
35316475	4	0	theme	water	752:756	arg1	volume					758:763	water volume	752:763	water volume in the culture medium	752:785	The variables optimized in the fermentation were temperature, agitation, pH, water volume in the culture medium, glucose concentration, and yeast extract.					
35316475	4	1	theme	yeast	815:819	arg1	extract					821:827	yeast extract	815:827	yeast extract	815:827	The variables optimized in the fermentation were temperature, agitation, pH, water volume in the culture medium, glucose concentration, and yeast extract.					
35316475	4	2	from	extract	821:827	arg1	medium					780:785	the culture medium	768:785	the culture medium	768:785	The variables optimized in the fermentation were temperature, agitation, pH, water volume in the culture medium, glucose concentration, and yeast extract.					
35316475	5	3	dep	bicolor	969:975	arg1	leaves					977:982	leaves	977:982	leaves	977:982	The fungus was grown on sugarcane bagasse substrate, and its metabolites were applied to detached Cucumis sativus, Conyza sp., and Sorghum bicolor leaves and used in an avoidance test and acute exposure to earthworms.					
35316475	5	3	dep	bicolor	969:975	arg1	used					988:991	used	988:991	used in an avoidance test and acute exposure to earthworms	988:1045	The fungus was grown on sugarcane bagasse substrate, and its metabolites were applied to detached Cucumis sativus, Conyza sp., and Sorghum bicolor leaves and used in an avoidance test and acute exposure to earthworms.					
35316475	4	4	from	pH	748:749	arg1	medium					780:785	the culture medium	768:785	the culture medium	768:785	The variables optimized in the fermentation were temperature, agitation, pH, water volume in the culture medium, glucose concentration, and yeast extract.					
35316475	5	5	theme	avoidance	999:1007	arg1	test					1009:1012	an avoidance test	996:1012	an avoidance test	996:1012	The fungus was grown on sugarcane bagasse substrate, and its metabolites were applied to detached Cucumis sativus, Conyza sp., and Sorghum bicolor leaves and used in an avoidance test and acute exposure to earthworms.					
35316475	0	6	theme	Ecotoxicological	102:117	arg1	Effects					119:125	its Ecotoxicological Effects	98:125	its Ecotoxicological Effects	98:125	Phytotoxicity Optimization of Fungal Metabolites Produced by Solid and Submerged Fermentation and its Ecotoxicological Effects.					
35316475	7	7	theme	113.92 ml kg-1	1350:1363	arg1	doses					1325:1329	the doses	1321:1329	the doses tested (maximum of 113.92 ml kg-1)	1321:1364	The metabolites severely damaged germination, initial growth, and leaves of the three plants, and at the doses tested (maximum of 113.92 ml kg-1), the metabolites of M. indicus UFSM 54 were not toxic to earthworms.					
35316475	7	7	theme	113.92 ml kg-1	1350:1363	arg1	maximum					1339:1345	maximum	1339:1345	maximum of 113.92 ml kg-1	1339:1363	The metabolites severely damaged germination, initial growth, and leaves of the three plants, and at the doses tested (maximum of 113.92 ml kg-1), the metabolites of M. indicus UFSM 54 were not toxic to earthworms.					
35316475	2	8	theme	environmental	316:328	arg1	impact					330:335	low environmental impact	312:335	low environmental impact	312:335	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	4	9	theme	glucose	788:794	arg1	concentration					796:808	glucose concentration	788:808	glucose concentration	788:808	The variables optimized in the fermentation were temperature, agitation, pH, water volume in the culture medium, glucose concentration, and yeast extract.					
35316475	7	10	theme	indicus	1389:1395	arg1	UFSM					1397:1400	M. indicus UFSM 54	1386:1403	M. indicus UFSM 54	1386:1403	The metabolites severely damaged germination, initial growth, and leaves of the three plants, and at the doses tested (maximum of 113.92 ml kg-1), the metabolites of M. indicus UFSM 54 were not toxic to earthworms.					
35316475	3	11	theme	central	591:597	arg1	designs					619:625	The Plackett-Burman and central composite rotatable designs	567:625	The Plackett-Burman and central composite rotatable designs	567:625	The Plackett-Burman and central composite rotatable designs were used to optimize metabolite phytotoxicity.					
35316475	7	12	theme	initial	1266:1272	arg1	growth					1274:1279	initial growth	1266:1279	initial growth	1266:1279	The metabolites severely damaged germination, initial growth, and leaves of the three plants, and at the doses tested (maximum of 113.92 ml kg-1), the metabolites of M. indicus UFSM 54 were not toxic to earthworms.					
35316475	2	13	theme	low	312:314	arg1	impact					330:335	low environmental impact	312:335	low environmental impact	312:335	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	4	14	from	agitation	737:745	arg1	medium					780:785	the culture medium	768:785	the culture medium	768:785	The variables optimized in the fermentation were temperature, agitation, pH, water volume in the culture medium, glucose concentration, and yeast extract.					
35316475	6	15	theme	submerged	1076:1084	arg1	fermentation					1086:1097	submerged fermentation	1076:1097	submerged fermentation	1076:1097	Metabolite phytotoxicity in submerged fermentation was optimized at 35 °C, 50 rpm, and 1.5 g l-1 of glucose and in solid fermentation at 30-37 °C and in 14-32 ml of water.					
35316475	5	16	dep	Conyza	945:950	arg1	sp.					952:954	Conyza sp.	945:954	Conyza sp.	945:954	The fungus was grown on sugarcane bagasse substrate, and its metabolites were applied to detached Cucumis sativus, Conyza sp., and Sorghum bicolor leaves and used in an avoidance test and acute exposure to earthworms.					
35316475	6	17	from	30-37 °C	1185:1192	arg1	35 °C					1116:1120	35 °C	1116:1120	35 °C	1116:1120	Metabolite phytotoxicity in submerged fermentation was optimized at 35 °C, 50 rpm, and 1.5 g l-1 of glucose and in solid fermentation at 30-37 °C and in 14-32 ml of water.					
35316475	2	18	theme	indicus	430:436	arg1	UFSM					438:441	the fungus Mycoleptodiscus indicus UFSM 54	403:444	the fungus Mycoleptodiscus indicus UFSM 54	403:444	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	2	19	theme	Eisenia	550:556	arg1	earthworms					538:547	earthworms	538:547	earthworms (Eisenia andrei)	538:564	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	2	19	theme	Eisenia	550:556	arg1	andrei					558:563	Eisenia andrei	550:563	Eisenia andrei	550:563	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	4	20	from	concentration	796:808	arg1	medium					780:785	the culture medium	768:785	the culture medium	768:785	The variables optimized in the fermentation were temperature, agitation, pH, water volume in the culture medium, glucose concentration, and yeast extract.					
35316475	0	21	theme	Phytotoxicity	0:12	arg1	Optimization					14:25	Phytotoxicity Optimization	0:25	Phytotoxicity Optimization of Fungal Metabolites	0:47	Phytotoxicity Optimization of Fungal Metabolites Produced by Solid and Submerged Fermentation and its Ecotoxicological Effects.					
35316475	4	22	from	temperature	724:734	arg1	medium					780:785	the culture medium	768:785	the culture medium	768:785	The variables optimized in the fermentation were temperature, agitation, pH, water volume in the culture medium, glucose concentration, and yeast extract.					
35316475	1	23	theme	lesser	191:196	arg1	extent					198:203	a lesser extent	189:203	a lesser extent	189:203	Research and commercial production of bioherbicides occur to a lesser extent compared to bioinsecticides and biofungicides.					
35316475	6	24	from	phytotoxicity	1059:1071	arg1	fermentation					1086:1097	submerged fermentation	1076:1097	submerged fermentation	1076:1097	Metabolite phytotoxicity in submerged fermentation was optimized at 35 °C, 50 rpm, and 1.5 g l-1 of glucose and in solid fermentation at 30-37 °C and in 14-32 ml of water.					
35316475	5	25	theme	Sorghum	961:967	arg1	bicolor					969:975	Sorghum bicolor	961:975	Sorghum bicolor leaves and used in an avoidance test and acute exposure to earthworms	961:1045	The fungus was grown on sugarcane bagasse substrate, and its metabolites were applied to detached Cucumis sativus, Conyza sp., and Sorghum bicolor leaves and used in an avoidance test and acute exposure to earthworms.					
35316475	2	26	theme	fungus	407:412	arg1	UFSM					438:441	the fungus Mycoleptodiscus indicus UFSM 54	403:444	the fungus Mycoleptodiscus indicus UFSM 54	403:444	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	2	27	theme	submerged	470:478	arg1	fermentation					480:491	solid and submerged fermentation	460:491	solid and submerged fermentation	460:491	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	7	28	theme	UFSM	1397:1400	arg1	toxic					1414:1418	toxic	1414:1418	toxic	1414:1418	The metabolites severely damaged germination, initial growth, and leaves of the three plants, and at the doses tested (maximum of 113.92 ml kg-1), the metabolites of M. indicus UFSM 54 were not toxic to earthworms.					
35316475	7	28	theme	UFSM	1397:1400	arg1	metabolites					1371:1381	the metabolites	1367:1381	the metabolites of M. indicus UFSM 54	1367:1403	The metabolites severely damaged germination, initial growth, and leaves of the three plants, and at the doses tested (maximum of 113.92 ml kg-1), the metabolites of M. indicus UFSM 54 were not toxic to earthworms.					
35316475	0	29	theme	Fungal	30:35	arg1	Metabolites					37:47	Fungal Metabolites	30:47	Fungal Metabolites	30:47	Phytotoxicity Optimization of Fungal Metabolites Produced by Solid and Submerged Fermentation and its Ecotoxicological Effects.					
35316475	6	30	theme	water	1213:1217	arg1	14-32 ml					1201:1208	14-32 ml	1201:1208	14-32 ml of water	1201:1217	Metabolite phytotoxicity in submerged fermentation was optimized at 35 °C, 50 rpm, and 1.5 g l-1 of glucose and in solid fermentation at 30-37 °C and in 14-32 ml of water.					
35316475	7	31	dep	tested	1331:1336	arg1	doses					1325:1329	the doses	1321:1329	the doses tested (maximum of 113.92 ml kg-1)	1321:1364	The metabolites severely damaged germination, initial growth, and leaves of the three plants, and at the doses tested (maximum of 113.92 ml kg-1), the metabolites of M. indicus UFSM 54 were not toxic to earthworms.					
35316475	7	31	dep	tested	1331:1336	arg1	maximum					1339:1345	maximum	1339:1345	maximum of 113.92 ml kg-1	1339:1363	The metabolites severely damaged germination, initial growth, and leaves of the three plants, and at the doses tested (maximum of 113.92 ml kg-1), the metabolites of M. indicus UFSM 54 were not toxic to earthworms.					
35316475	3	32	theme	composite	599:607	arg1	designs					619:625	The Plackett-Burman and central composite rotatable designs	567:625	The Plackett-Burman and central composite rotatable designs	567:625	The Plackett-Burman and central composite rotatable designs were used to optimize metabolite phytotoxicity.					
35316475	2	33	theme	solid	460:464	arg1	fermentation					480:491	solid and submerged fermentation	460:491	solid and submerged fermentation	460:491	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	3	34	theme	rotatable	609:617	arg1	designs					619:625	The Plackett-Burman and central composite rotatable designs	567:625	The Plackett-Burman and central composite rotatable designs	567:625	The Plackett-Burman and central composite rotatable designs were used to optimize metabolite phytotoxicity.					
35316475	5	35	theme	sugarcane	854:862	arg1	substrate					872:880	sugarcane bagasse substrate	854:880	sugarcane bagasse substrate	854:880	The fungus was grown on sugarcane bagasse substrate, and its metabolites were applied to detached Cucumis sativus, Conyza sp., and Sorghum bicolor leaves and used in an avoidance test and acute exposure to earthworms.					
35316475	2	36	theme	ecotoxicological	510:525	arg1	effects					527:533	the ecotoxicological effects	506:533	the ecotoxicological effects on earthworms (Eisenia andrei)	506:564	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	6	37	theme	glucose	1148:1154	arg1	35 °C					1116:1120	35 °C	1116:1120	35 °C	1116:1120	Metabolite phytotoxicity in submerged fermentation was optimized at 35 °C, 50 rpm, and 1.5 g l-1 of glucose and in solid fermentation at 30-37 °C and in 14-32 ml of water.					
35316475	2	38	from	effects	527:533	arg1	earthworms					538:547	earthworms	538:547	earthworms (Eisenia andrei)	538:564	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	2	38	from	effects	527:533	arg1	andrei					558:563	Eisenia andrei	550:563	Eisenia andrei	550:563	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	0	39	theme	Metabolites	37:47	arg1	Optimization					14:25	Phytotoxicity Optimization	0:25	Phytotoxicity Optimization of Fungal Metabolites	0:47	Phytotoxicity Optimization of Fungal Metabolites Produced by Solid and Submerged Fermentation and its Ecotoxicological Effects.					
35316475	3	40	theme	metabolite	649:658	arg1	phytotoxicity					660:672	metabolite phytotoxicity	649:672	metabolite phytotoxicity	649:672	The Plackett-Burman and central composite rotatable designs were used to optimize metabolite phytotoxicity.					
35316475	2	41	theme	UFSM	438:441	arg1	metabolites					388:398	metabolites	388:398	metabolites of the fungus Mycoleptodiscus indicus UFSM 54	388:444	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	0	42	theme	Solid	61:65	arg1	Fermentation					81:92	Solid and Submerged Fermentation	61:92	Solid and Submerged Fermentation	61:92	Phytotoxicity Optimization of Fungal Metabolites Produced by Solid and Submerged Fermentation and its Ecotoxicological Effects.					
35316475	5	43	theme	detached	919:926	arg1	sativus					936:942	Cucumis sativus	928:942	Cucumis sativus	928:942	The fungus was grown on sugarcane bagasse substrate, and its metabolites were applied to detached Cucumis sativus, Conyza sp., and Sorghum bicolor leaves and used in an avoidance test and acute exposure to earthworms.					
35316475	7	44	theme	M.	1386:1387	arg1	UFSM					1397:1400	M. indicus UFSM 54	1386:1403	M. indicus UFSM 54	1386:1403	The metabolites severely damaged germination, initial growth, and leaves of the three plants, and at the doses tested (maximum of 113.92 ml kg-1), the metabolites of M. indicus UFSM 54 were not toxic to earthworms.					
35316475	3	45	used	used	632:635	arg2	designs					619:625	The Plackett-Burman and central composite rotatable designs	567:625	The Plackett-Burman and central composite rotatable designs	567:625	The Plackett-Burman and central composite rotatable designs were used to optimize metabolite phytotoxicity.					
35316475	4	46	theme	culture	772:778	arg1	medium					780:785	the culture medium	768:785	the culture medium	768:785	The variables optimized in the fermentation were temperature, agitation, pH, water volume in the culture medium, glucose concentration, and yeast extract.					
35316475	2	47	dep	fungus	407:412	arg1	Mycoleptodiscus					414:428	Mycoleptodiscus	414:428	Mycoleptodiscus	414:428	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	2	48	theme	new	289:291	arg1	bioherbicides					293:305	developing new bioherbicides	278:305	developing new bioherbicides with low environmental impact	278:335	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	1	49	theme	commercial	141:150	arg1	production					152:161	commercial production	141:161	commercial production	141:161	Research and commercial production of bioherbicides occur to a lesser extent compared to bioinsecticides and biofungicides.					
35316475	6	50	theme	solid	1163:1167	arg1	fermentation					1169:1180	solid fermentation	1163:1180	solid fermentation	1163:1180	Metabolite phytotoxicity in submerged fermentation was optimized at 35 °C, 50 rpm, and 1.5 g l-1 of glucose and in solid fermentation at 30-37 °C and in 14-32 ml of water.					
35316475	6	51	theme	Metabolite	1048:1057	arg1	phytotoxicity					1059:1071	Metabolite phytotoxicity	1048:1071	Metabolite phytotoxicity in submerged fermentation	1048:1097	Metabolite phytotoxicity in submerged fermentation was optimized at 35 °C, 50 rpm, and 1.5 g l-1 of glucose and in solid fermentation at 30-37 °C and in 14-32 ml of water.					
35316475	3	52	theme	Plackett-Burman	571:585	arg1	designs					619:625	The Plackett-Burman and central composite rotatable designs	567:625	The Plackett-Burman and central composite rotatable designs	567:625	The Plackett-Burman and central composite rotatable designs were used to optimize metabolite phytotoxicity.					
35316475	2	53	theme	developing	278:287	arg1	bioherbicides					293:305	developing new bioherbicides	278:305	developing new bioherbicides with low environmental impact	278:335	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	1	54	theme	bioherbicides	166:178	arg1	Research					128:135	Research	128:135	Research	128:135	Research and commercial production of bioherbicides occur to a lesser extent compared to bioinsecticides and biofungicides.					
35316475	1	54	theme	bioherbicides	166:178	arg1	production					152:161	commercial production	141:161	commercial production	141:161	Research and commercial production of bioherbicides occur to a lesser extent compared to bioinsecticides and biofungicides.					
35316475	2	55	with	bioherbicides	293:305	arg1	impact					330:335	low environmental impact	312:335	low environmental impact	312:335	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35316475	0	56	theme	Submerged	71:79	arg1	Fermentation					81:92	Solid and Submerged Fermentation	61:92	Solid and Submerged Fermentation	61:92	Phytotoxicity Optimization of Fungal Metabolites Produced by Solid and Submerged Fermentation and its Ecotoxicological Effects.					
35316475	5	57	theme	acute	1018:1022	arg1	exposure					1024:1031	acute exposure	1018:1031	acute exposure to earthworms	1018:1045	The fungus was grown on sugarcane bagasse substrate, and its metabolites were applied to detached Cucumis sativus, Conyza sp., and Sorghum bicolor leaves and used in an avoidance test and acute exposure to earthworms.					
35316475	5	58	theme	Cucumis	928:934	arg1	sativus					936:942	Cucumis sativus	928:942	Cucumis sativus	928:942	The fungus was grown on sugarcane bagasse substrate, and its metabolites were applied to detached Cucumis sativus, Conyza sp., and Sorghum bicolor leaves and used in an avoidance test and acute exposure to earthworms.					
35316475	4	59	from	volume	758:763	arg1	medium					780:785	the culture medium	768:785	the culture medium	768:785	The variables optimized in the fermentation were temperature, agitation, pH, water volume in the culture medium, glucose concentration, and yeast extract.					
35316475	5	60	theme	bagasse	864:870	arg1	substrate					872:880	sugarcane bagasse substrate	854:880	sugarcane bagasse substrate	854:880	The fungus was grown on sugarcane bagasse substrate, and its metabolites were applied to detached Cucumis sativus, Conyza sp., and Sorghum bicolor leaves and used in an avoidance test and acute exposure to earthworms.					
35316475	7	61	from	doses	1325:1329	arg1	metabolites					1371:1381	the metabolites	1367:1381	the metabolites of M. indicus UFSM 54	1367:1403	The metabolites severely damaged germination, initial growth, and leaves of the three plants, and at the doses tested (maximum of 113.92 ml kg-1), the metabolites of M. indicus UFSM 54 were not toxic to earthworms.					
35316475	7	61	from	doses	1325:1329	arg1	toxic					1414:1418	toxic	1414:1418	toxic	1414:1418	The metabolites severely damaged germination, initial growth, and leaves of the three plants, and at the doses tested (maximum of 113.92 ml kg-1), the metabolites of M. indicus UFSM 54 were not toxic to earthworms.					
35316475	2	62	theme	metabolites	388:398	arg1	phytotoxicity					371:383	the phytotoxicity	367:383	the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54	367:444	In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms (Eisenia andrei).					
35850109	0	0	theme	Acid-Modified	88:100	arg1	Nanocarriers					102:113	Hyaluronic Acid-Modified Nanocarriers	77:113	Hyaluronic Acid-Modified Nanocarriers	77:113	B16F10 Cell Membrane-Based Nanovesicles for Melanoma Therapy Are Superior to Hyaluronic Acid-Modified Nanocarriers.					
35850109	5	1	theme	hyaluronic	1173:1182	arg1	acid					1184:1187	hyaluronic acid	1173:1187	hyaluronic acid	1173:1187	In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid.					
35850109	2	2	theme	nanocrown	357:365	arg1	structure					367:375	a composite nanocrown structure	345:375	a composite nanocrown structure	345:375	By co-constructing the B16F10 cell membrane with a PEGylated phospholipid membrane, a new nanocarrier with a composite nanocrown structure was developed, which can evade immune recognition and actively target homologous melanoma.					
35850109	4	3	theme	reticuloendothelial	799:817	arg1	cells					819:823	reticuloendothelial cells	799:823	reticuloendothelial cells	799:823	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	5	4	theme	nanocrowns	1257:1266	arg1	capacity					1220:1227	targeting capacity	1210:1227	targeting capacity	1210:1227	In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid.					
35850109	5	4	theme	nanocrowns	1257:1266	arg1	effects					1242:1248	antitumor effects	1232:1248	antitumor effects	1232:1248	In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid.					
35850109	0	5	theme	Hyaluronic	77:86	arg1	Nanocarriers					102:113	Hyaluronic Acid-Modified Nanocarriers	77:113	Hyaluronic Acid-Modified Nanocarriers	77:113	B16F10 Cell Membrane-Based Nanovesicles for Melanoma Therapy Are Superior to Hyaluronic Acid-Modified Nanocarriers.					
35850109	4	6	from	cells	819:823	arg1	escape					771:776	the escape	767:776	the escape of nanovesicles from reticuloendothelial cells	767:823	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	2	7	theme	composite	347:355	arg1	structure					367:375	a composite nanocrown structure	345:375	a composite nanocrown structure	345:375	By co-constructing the B16F10 cell membrane with a PEGylated phospholipid membrane, a new nanocarrier with a composite nanocrown structure was developed, which can evade immune recognition and actively target homologous melanoma.					
35850109	4	8	theme	paclitaxel	996:1005	arg1	effects					985:991	anticancer effects	974:991	anticancer effects of paclitaxel	974:1005	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	3	9	theme	phospholipid	615:626	arg1	vesicles					637:644	the PEGylated phospholipid membrane vesicles	601:644	the PEGylated phospholipid membrane vesicles	601:644	The nanocrowns have an encapsulation efficiency of more than 90% for paclitaxel and showed no significant difference (p > 0.05) from the PEGylated phospholipid membrane vesicles.					
35850109	4	10	theme	superior	909:916	arg1	capability					950:959	superior melanoma-targeted drug delivery capability	909:959	superior melanoma-targeted drug delivery capability	909:959	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	3	11	theme	p	586:586	arg1	difference					574:583	no significant difference	559:583	no significant difference (p > 0.05)	559:594	The nanocrowns have an encapsulation efficiency of more than 90% for paclitaxel and showed no significant difference (p > 0.05) from the PEGylated phospholipid membrane vesicles.					
35850109	3	11	theme	p	586:586	arg1	>					588:588	p > 0.05	586:593	p > 0.05	586:593	The nanocrowns have an encapsulation efficiency of more than 90% for paclitaxel and showed no significant difference (p > 0.05) from the PEGylated phospholipid membrane vesicles.					
35850109	2	12	theme	PEGylated	289:297	arg1	membrane					312:319	a PEGylated phospholipid membrane	287:319	a PEGylated phospholipid membrane	287:319	By co-constructing the B16F10 cell membrane with a PEGylated phospholipid membrane, a new nanocarrier with a composite nanocrown structure was developed, which can evade immune recognition and actively target homologous melanoma.					
35850109	2	13	theme	phospholipid	299:310	arg1	membrane					312:319	a PEGylated phospholipid membrane	287:319	a PEGylated phospholipid membrane	287:319	By co-constructing the B16F10 cell membrane with a PEGylated phospholipid membrane, a new nanocarrier with a composite nanocrown structure was developed, which can evade immune recognition and actively target homologous melanoma.					
35850109	5	14	dep	capacity	1220:1227	arg1	the					1206:1208	the	1206:1208	the	1206:1208	In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid.					
35850109	4	15	theme	nanovesicles	781:792	arg1	escape					771:776	the escape	767:776	the escape of nanovesicles from reticuloendothelial cells	767:823	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	2	16	theme	new	324:326	arg1	nanocarrier					328:338	a new nanocarrier	322:338	a new nanocarrier with a composite nanocrown structure	322:375	By co-constructing the B16F10 cell membrane with a PEGylated phospholipid membrane, a new nanocarrier with a composite nanocrown structure was developed, which can evade immune recognition and actively target homologous melanoma.					
35850109	4	17	theme	membrane	713:720	arg1	vesicles					722:729	the hyaluronic acid-modified PEGylated phospholipid membrane vesicles	661:729	the hyaluronic acid-modified PEGylated phospholipid membrane vesicles	661:729	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	1	18	theme	strong	174:179	arg1	effects					188:194	strong homing effects	174:194	strong homing effects	174:194	Some cancer cell membrane (CCM)-derived nanovesicles show strong homing effects and are used for targeted cancer therapy.					
35850109	6	19	theme	melanoma-targeted	1473:1489	arg1	therapy					1491:1497	active melanoma-targeted therapy	1466:1497	active melanoma-targeted therapy	1466:1497	Our results demonstrate the potential of using biomimetic nanocrowns for active melanoma-targeted therapy.					
35850109	4	20	theme	cell	1051:1054	arg1	proliferation					1056:1068	B16F10 cell proliferation	1044:1068	B16F10 cell proliferation	1044:1068	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	2	21	theme	homologous	447:456	arg1	melanoma					458:465	homologous melanoma	447:465	homologous melanoma	447:465	By co-constructing the B16F10 cell membrane with a PEGylated phospholipid membrane, a new nanocarrier with a composite nanocrown structure was developed, which can evade immune recognition and actively target homologous melanoma.					
35850109	1	22	theme	homing	181:186	arg1	effects					188:194	strong homing effects	174:194	strong homing effects	174:194	Some cancer cell membrane (CCM)-derived nanovesicles show strong homing effects and are used for targeted cancer therapy.					
35850109	0	23	theme	Cell	7:10	arg1	Nanovesicles					27:38	B16F10 Cell Membrane-Based Nanovesicles	0:38	B16F10 Cell Membrane-Based Nanovesicles for Melanoma Therapy	0:59	B16F10 Cell Membrane-Based Nanovesicles for Melanoma Therapy Are Superior to Hyaluronic Acid-Modified Nanocarriers.					
35850109	3	24	theme	%	531:531	arg1	efficiency					505:514	an encapsulation efficiency	488:514	an encapsulation efficiency of more than 90% for paclitaxel	488:546	The nanocrowns have an encapsulation efficiency of more than 90% for paclitaxel and showed no significant difference (p > 0.05) from the PEGylated phospholipid membrane vesicles.					
35850109	5	25	theme	antitumor	1232:1240	arg1	effects					1242:1248	antitumor effects	1232:1248	antitumor effects	1232:1248	In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid.					
35850109	4	26	theme	acid-modified	676:688	arg1	vesicles					722:729	the hyaluronic acid-modified PEGylated phospholipid membrane vesicles	661:729	the hyaluronic acid-modified PEGylated phospholipid membrane vesicles	661:729	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	0	27	theme	B16F10	0:5	arg1	Nanovesicles					27:38	B16F10 Cell Membrane-Based Nanovesicles	0:38	B16F10 Cell Membrane-Based Nanovesicles for Melanoma Therapy	0:59	B16F10 Cell Membrane-Based Nanovesicles for Melanoma Therapy Are Superior to Hyaluronic Acid-Modified Nanocarriers.					
35850109	3	28	theme	significant	562:572	arg1	difference					574:583	no significant difference	559:583	no significant difference (p > 0.05)	559:594	The nanocrowns have an encapsulation efficiency of more than 90% for paclitaxel and showed no significant difference (p > 0.05) from the PEGylated phospholipid membrane vesicles.					
35850109	3	28	theme	significant	562:572	arg1	>					588:588	p > 0.05	586:593	p > 0.05	586:593	The nanocrowns have an encapsulation efficiency of more than 90% for paclitaxel and showed no significant difference (p > 0.05) from the PEGylated phospholipid membrane vesicles.					
35850109	4	29	theme	biomimetic	736:745	arg1	nanocrowns					747:756	the biomimetic nanocrowns	732:756	the biomimetic nanocrowns	732:756	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	4	30	theme	induction	1074:1082	arg1	inhibition					1030:1039	the inhibition	1026:1039	the inhibition of B16F10 cell proliferation and induction of apoptosis	1026:1095	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	4	31	theme	hyaluronic	665:674	arg1	vesicles					722:729	the hyaluronic acid-modified PEGylated phospholipid membrane vesicles	661:729	the hyaluronic acid-modified PEGylated phospholipid membrane vesicles	661:729	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	6	32	theme	biomimetic	1440:1449	arg1	nanocrowns					1451:1460	biomimetic nanocrowns	1440:1460	biomimetic nanocrowns for active melanoma-targeted therapy	1440:1497	Our results demonstrate the potential of using biomimetic nanocrowns for active melanoma-targeted therapy.					
35850109	0	33	theme	Membrane-Based	12:25	arg1	Nanovesicles					27:38	B16F10 Cell Membrane-Based Nanovesicles	0:38	B16F10 Cell Membrane-Based Nanovesicles for Melanoma Therapy	0:59	B16F10 Cell Membrane-Based Nanovesicles for Melanoma Therapy Are Superior to Hyaluronic Acid-Modified Nanocarriers.					
35850109	2	34	with	nanocarrier	328:338	arg1	structure					367:375	a composite nanocrown structure	345:375	a composite nanocrown structure	345:375	By co-constructing the B16F10 cell membrane with a PEGylated phospholipid membrane, a new nanocarrier with a composite nanocrown structure was developed, which can evade immune recognition and actively target homologous melanoma.					
35850109	1	35	theme	cancer	121:126	arg1	nanovesicles					156:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles show strong homing effects and are used for targeted cancer therapy.					
35850109	3	36	theme	membrane	628:635	arg1	vesicles					637:644	the PEGylated phospholipid membrane vesicles	601:644	the PEGylated phospholipid membrane vesicles	601:644	The nanocrowns have an encapsulation efficiency of more than 90% for paclitaxel and showed no significant difference (p > 0.05) from the PEGylated phospholipid membrane vesicles.					
35850109	0	37	theme	Melanoma	44:51	arg1	Therapy					53:59	Melanoma Therapy	44:59	Melanoma Therapy	44:59	B16F10 Cell Membrane-Based Nanovesicles for Melanoma Therapy Are Superior to Hyaluronic Acid-Modified Nanocarriers.					
35850109	4	38	theme	circulation	851:861	arg1	time					863:866	the circulation time	847:866	the circulation time	847:866	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	2	39	theme	immune	408:413	arg1	recognition					415:425	immune recognition	408:425	immune recognition	408:425	By co-constructing the B16F10 cell membrane with a PEGylated phospholipid membrane, a new nanocarrier with a composite nanocrown structure was developed, which can evade immune recognition and actively target homologous melanoma.					
35850109	4	40	theme	B16F10	1044:1049	arg1	proliferation					1056:1068	B16F10 cell proliferation	1044:1068	B16F10 cell proliferation	1044:1068	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	1	41	link	-derived	147:154	arg1	nanovesicles					156:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles show strong homing effects and are used for targeted cancer therapy.					
35850109	3	42	contain	have	483:486	arg1	nanocrowns					472:481	The nanocrowns	468:481	The nanocrowns	468:481	The nanocrowns have an encapsulation efficiency of more than 90% for paclitaxel and showed no significant difference (p > 0.05) from the PEGylated phospholipid membrane vesicles.					
35850109	3	42	contain	have	483:486	arg2	efficiency					505:514	an encapsulation efficiency	488:514	an encapsulation efficiency of more than 90% for paclitaxel	488:546	The nanocrowns have an encapsulation efficiency of more than 90% for paclitaxel and showed no significant difference (p > 0.05) from the PEGylated phospholipid membrane vesicles.					
35850109	5	43	theme	targeting	1298:1306	arg1	capacity					1308:1315	the superior targeting capacity	1285:1315	the superior targeting capacity	1285:1315	In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid.					
35850109	1	44	theme	targeted	213:220	arg1	therapy					229:235	targeted cancer therapy	213:235	targeted cancer therapy	213:235	Some cancer cell membrane (CCM)-derived nanovesicles show strong homing effects and are used for targeted cancer therapy.					
35850109	5	45	theme	targeting	1210:1218	arg1	capacity					1220:1227	targeting capacity	1210:1227	targeting capacity	1210:1227	In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid.					
35850109	4	46	theme	drug	936:939	arg1	capability					950:959	superior melanoma-targeted drug delivery capability	909:959	superior melanoma-targeted drug delivery capability	909:959	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	1	47	used	used	204:207	arg2	nanovesicles					156:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles show strong homing effects and are used for targeted cancer therapy.					
35850109	1	48	theme	cell	128:131	arg1	nanovesicles					156:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles show strong homing effects and are used for targeted cancer therapy.					
35850109	4	49	theme	proliferation	1056:1068	arg1	inhibition					1030:1039	the inhibition	1026:1039	the inhibition of B16F10 cell proliferation and induction of apoptosis	1026:1095	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	4	50	theme	melanoma-targeted	918:934	arg1	capability					950:959	superior melanoma-targeted drug delivery capability	909:959	superior melanoma-targeted drug delivery capability	909:959	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	1	51	theme	membrane	133:140	arg1	nanovesicles					156:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles show strong homing effects and are used for targeted cancer therapy.					
35850109	5	52	theme	exposed	1337:1343	arg1	CCMs					1356:1359	the exposed homologous CCMs	1333:1359	the exposed homologous CCMs	1333:1359	In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid.					
35850109	3	53	theme	PEGylated	605:613	arg1	vesicles					637:644	the PEGylated phospholipid membrane vesicles	601:644	the PEGylated phospholipid membrane vesicles	601:644	The nanocrowns have an encapsulation efficiency of more than 90% for paclitaxel and showed no significant difference (p > 0.05) from the PEGylated phospholipid membrane vesicles.					
35850109	2	54	theme	cell	268:271	arg1	membrane					273:280	the B16F10 cell membrane	257:280	the B16F10 cell membrane	257:280	By co-constructing the B16F10 cell membrane with a PEGylated phospholipid membrane, a new nanocarrier with a composite nanocrown structure was developed, which can evade immune recognition and actively target homologous melanoma.					
35850109	4	55	theme	PEGylated	690:698	arg1	vesicles					722:729	the hyaluronic acid-modified PEGylated phospholipid membrane vesicles	661:729	the hyaluronic acid-modified PEGylated phospholipid membrane vesicles	661:729	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	5	56	theme	homologous	1345:1354	arg1	CCMs					1356:1359	the exposed homologous CCMs	1333:1359	the exposed homologous CCMs	1333:1359	In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid.					
35850109	1	57	theme	cancer	222:227	arg1	therapy					229:235	targeted cancer therapy	213:235	targeted cancer therapy	213:235	Some cancer cell membrane (CCM)-derived nanovesicles show strong homing effects and are used for targeted cancer therapy.					
35850109	4	58	theme	delivery	941:948	arg1	capability					950:959	superior melanoma-targeted drug delivery capability	909:959	superior melanoma-targeted drug delivery capability	909:959	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	2	59	theme	B16F10	261:266	arg1	membrane					273:280	the B16F10 cell membrane	257:280	the B16F10 cell membrane	257:280	By co-constructing the B16F10 cell membrane with a PEGylated phospholipid membrane, a new nanocarrier with a composite nanocrown structure was developed, which can evade immune recognition and actively target homologous melanoma.					
35850109	1	60	theme	CCM	143:145	arg1	nanovesicles					156:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles show strong homing effects and are used for targeted cancer therapy.					
35850109	5	61	theme	superior	1289:1296	arg1	capacity					1308:1315	the superior targeting capacity	1285:1315	the superior targeting capacity	1285:1315	In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid.					
35850109	6	62	theme	active	1466:1471	arg1	therapy					1491:1497	active melanoma-targeted therapy	1466:1497	active melanoma-targeted therapy	1466:1497	Our results demonstrate the potential of using biomimetic nanocrowns for active melanoma-targeted therapy.					
35850109	4	63	theme	microtubule	1117:1127	arg1	formation					1129:1137	microtubule formation	1117:1137	microtubule formation	1117:1137	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	4	64	theme	phospholipid	700:711	arg1	vesicles					722:729	the hyaluronic acid-modified PEGylated phospholipid membrane vesicles	661:729	the hyaluronic acid-modified PEGylated phospholipid membrane vesicles	661:729	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	3	65	theme	encapsulation	491:503	arg1	efficiency					505:514	an encapsulation efficiency	488:514	an encapsulation efficiency of more than 90% for paclitaxel	488:546	The nanocrowns have an encapsulation efficiency of more than 90% for paclitaxel and showed no significant difference (p > 0.05) from the PEGylated phospholipid membrane vesicles.					
35850109	4	66	theme	anticancer	974:983	arg1	effects					985:991	anticancer effects	974:991	anticancer effects of paclitaxel	974:1005	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	5	67	theme	acid	1184:1187	arg1	modification					1157:1168	the modification	1153:1168	the modification of hyaluronic acid	1153:1187	In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid.					
35850109	4	68	theme	apoptosis	1087:1095	arg1	proliferation					1056:1068	B16F10 cell proliferation	1044:1068	B16F10 cell proliferation	1044:1068	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	4	68	theme	apoptosis	1087:1095	arg1	induction					1074:1082	induction	1074:1082	induction of apoptosis	1074:1095	Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation.					
35850109	5	69	theme	hyaluronic	1376:1385	arg1	acid					1387:1390	hyaluronic acid	1376:1390	hyaluronic acid	1376:1390	In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid.					
35850109	1	70	theme	-derived	147:154	arg1	nanovesicles					156:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles	116:167	Some cancer cell membrane (CCM)-derived nanovesicles show strong homing effects and are used for targeted cancer therapy.					
37352715	3	0	theme	proteins	769:776	arg1	behavior					753:760	phase behavior	747:760	phase behavior	747:760	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	3	0	theme	proteins	769:776	arg1	structure					733:741	structure	733:741	structure	733:741	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	6	1	theme	high-tech	1158:1166	arg1	components					1189:1198	high-tech composite functional components	1158:1198	high-tech composite functional components	1158:1198	This study provides a theoretical basis and experimental foundation for the effective use of high-tech composite functional components to improve the quality of gel products.					
37352715	0	2	theme	protein	93:99	arg1	properties					32:41	the gel properties	24:41	the gel properties of transglutaminase-mediated porcine myofibrillar protein	24:99	Mechanism for improving the gel properties of transglutaminase-mediated porcine myofibrillar protein by ultrasonic pretreatment combined with carrageenan.					
37352715	1	3	theme	gel	363:365	arg1	properties					367:376	the gel properties	359:376	the gel properties of transglutaminase-mediated MP gels	359:413	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	4	4	theme	MP	924:925	arg1	gels					927:930	composite MP gels	914:930	composite MP gels	914:930	The synergistic effect also improved the formation of dense and interpenetrating gel networks, which reduced cooking loss and produced composite MP gels with optimal gel strength.					
37352715	1	5	theme	transglutaminase	184:199	arg1	limitation					170:179	the limitation	166:179	the limitation of transglutaminase	166:199	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	3	6	theme	phase	747:751	arg1	behavior					753:760	phase behavior	747:760	phase behavior	747:760	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	4	7	theme	optimal	937:943	arg1	strength					949:956	optimal gel strength	937:956	optimal gel strength	937:956	The synergistic effect also improved the formation of dense and interpenetrating gel networks, which reduced cooking loss and produced composite MP gels with optimal gel strength.					
37352715	5	8	theme	interactions	1026:1037	arg1	presence					1000:1007	the presence	996:1007	the presence of electrostatic interactions in the hybrid gel system	996:1062	Moreover, FTIR spectroscopy revealed the presence of electrostatic interactions in the hybrid gel system.					
37352715	0	9	theme	ultrasonic	104:113	arg1	pretreatment					115:126	ultrasonic pretreatment	104:126	ultrasonic pretreatment	104:126	Mechanism for improving the gel properties of transglutaminase-mediated porcine myofibrillar protein by ultrasonic pretreatment combined with carrageenan.					
37352715	6	10	theme	gel	1226:1228	arg1	products					1230:1237	gel products	1226:1237	gel products	1226:1237	This study provides a theoretical basis and experimental foundation for the effective use of high-tech composite functional components to improve the quality of gel products.					
37352715	1	11	from	combination	327:337	arg1	effect					290:295	the synergistic effect	274:295	the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels	274:413	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	3	12	theme	synergistic	594:604	arg1	effect					606:611	the synergistic effect	590:611	the synergistic effect	590:611	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	1	13	theme	synergistic	278:288	arg1	effect					290:295	the synergistic effect	274:295	the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels	274:413	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	1	14	theme	transglutaminase-mediated	381:405	arg1	gels					410:413	transglutaminase-mediated MP gels	381:413	transglutaminase-mediated MP gels	381:413	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	1	15	with	combination	327:337	arg1	carrageenan					344:354	carrageenan	344:354	carrageenan	344:354	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	3	16	dep	structure	543:551	arg1	results					553:559	results	553:559	results	553:559	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	6	17	theme	experimental	1109:1120	arg1	foundation					1122:1131	a theoretical basis and experimental foundation	1085:1131	a theoretical basis and experimental foundation for the effective use of high-tech composite functional components to improve the quality of gel products	1085:1237	This study provides a theoretical basis and experimental foundation for the effective use of high-tech composite functional components to improve the quality of gel products.					
37352715	3	18	dep	structure	733:741	arg1	the					729:731	the	729:731	the	729:731	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	1	19	theme	MP	407:408	arg1	gels					410:413	transglutaminase-mediated MP gels	381:413	transglutaminase-mediated MP gels	381:413	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	1	20	from	pretreatment	311:322	arg1	combination					327:337	combination	327:337	combination with carrageenan	327:354	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	5	21	theme	hybrid	1046:1051	arg1	system					1057:1062	the hybrid gel system	1042:1062	the hybrid gel system	1042:1062	Moreover, FTIR spectroscopy revealed the presence of electrostatic interactions in the hybrid gel system.					
37352715	2	22	theme	synergistic	420:430	arg1	effect					432:437	The synergistic effect	416:437	The synergistic effect	416:437	The synergistic effect generated gel with lower surface hydrophobicity and fluorescence intensity.					
37352715	2	23	theme	fluorescence	491:502	arg1	intensity					504:512	fluorescence intensity	491:512	fluorescence intensity	491:512	The synergistic effect generated gel with lower surface hydrophobicity and fluorescence intensity.					
37352715	1	24	theme	gels	410:413	arg1	properties					367:376	the gel properties	359:376	the gel properties of transglutaminase-mediated MP gels	359:413	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	1	25	from	effect	290:295	arg1	combination					327:337	combination	327:337	combination with carrageenan	327:354	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	1	25	from	effect	290:295	arg1	properties					367:376	the gel properties	359:376	the gel properties of transglutaminase-mediated MP gels	359:413	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	6	26	theme	products	1230:1237	arg1	quality					1215:1221	the quality	1211:1221	the quality of gel products	1211:1237	This study provides a theoretical basis and experimental foundation for the effective use of high-tech composite functional components to improve the quality of gel products.					
37352715	4	27	theme	synergistic	783:793	arg1	effect					795:800	The synergistic effect	779:800	The synergistic effect	779:800	The synergistic effect also improved the formation of dense and interpenetrating gel networks, which reduced cooking loss and produced composite MP gels with optimal gel strength.					
37352715	6	28	theme	effective	1141:1149	arg1	use					1151:1153	the effective use	1137:1153	the effective use of high-tech composite functional components to improve the quality of gel products	1137:1237	This study provides a theoretical basis and experimental foundation for the effective use of high-tech composite functional components to improve the quality of gel products.					
37352715	1	29	theme	myofibrillar	219:230	arg1	protein					232:238	myofibrillar protein	219:238	myofibrillar protein (MP) gel	219:247	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	1	29	theme	myofibrillar	219:230	arg1	MP					241:242	MP	241:242	MP	241:242	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	2	30	theme	surface	464:470	arg1	hydrophobicity					472:485	lower surface hydrophobicity	458:485	lower surface hydrophobicity	458:485	The synergistic effect generated gel with lower surface hydrophobicity and fluorescence intensity.					
37352715	1	31	theme	ultrasonic	300:309	arg1	pretreatment					311:322	ultrasonic pretreatment	300:322	ultrasonic pretreatment in combination with carrageenan	300:354	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	4	32	theme	dense	833:837	arg1	networks					864:871	dense and interpenetrating gel networks	833:871	dense and interpenetrating gel networks	833:871	The synergistic effect also improved the formation of dense and interpenetrating gel networks, which reduced cooking loss and produced composite MP gels with optimal gel strength.					
37352715	5	33	theme	electrostatic	1012:1024	arg1	interactions					1026:1037	electrostatic interactions	1012:1037	electrostatic interactions	1012:1037	Moreover, FTIR spectroscopy revealed the presence of electrostatic interactions in the hybrid gel system.					
37352715	1	34	theme	protein	232:238	arg1	gel					245:247	myofibrillar protein (MP) gel	219:247	myofibrillar protein (MP) gel	219:247	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	1	35	theme	pretreatment	311:322	arg1	effect					290:295	the synergistic effect	274:295	the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels	274:413	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	3	36	theme	proteins	645:652	arg1	formation					662:670	the formation	658:670	the formation of aggregates wrapping hydrophobic groups	658:712	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	3	36	theme	proteins	645:652	arg1	rearrangement					624:636	the rearrangement	620:636	the rearrangement of the proteins	620:652	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	0	37	theme	gel	28:30	arg1	properties					32:41	the gel properties	24:41	the gel properties of transglutaminase-mediated porcine myofibrillar protein	24:99	Mechanism for improving the gel properties of transglutaminase-mediated porcine myofibrillar protein by ultrasonic pretreatment combined with carrageenan.					
37352715	5	38	attach	presence	1000:1007	arg2	interactions					1026:1037	electrostatic interactions	1012:1037	electrostatic interactions	1012:1037	Moreover, FTIR spectroscopy revealed the presence of electrostatic interactions in the hybrid gel system.					
37352715	5	38	attach	presence	1000:1007	arg1	system					1057:1062	the hybrid gel system	1042:1062	the hybrid gel system	1042:1062	Moreover, FTIR spectroscopy revealed the presence of electrostatic interactions in the hybrid gel system.					
37352715	6	39	theme	basis	1099:1103	arg1	foundation					1122:1131	a theoretical basis and experimental foundation	1085:1131	a theoretical basis and experimental foundation for the effective use of high-tech composite functional components to improve the quality of gel products	1085:1237	This study provides a theoretical basis and experimental foundation for the effective use of high-tech composite functional components to improve the quality of gel products.					
37352715	0	40	theme	transglutaminase-mediated	46:70	arg1	protein					93:99	transglutaminase-mediated porcine myofibrillar protein	46:99	transglutaminase-mediated porcine myofibrillar protein	46:99	Mechanism for improving the gel properties of transglutaminase-mediated porcine myofibrillar protein by ultrasonic pretreatment combined with carrageenan.					
37352715	3	41	theme	hydrophobic	695:705	arg1	groups					707:712	hydrophobic groups	695:712	hydrophobic groups	695:712	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	4	42	theme	cooking	888:894	arg1	loss					896:899	cooking loss	888:899	cooking loss	888:899	The synergistic effect also improved the formation of dense and interpenetrating gel networks, which reduced cooking loss and produced composite MP gels with optimal gel strength.					
37352715	4	43	theme	gel	945:947	arg1	strength					949:956	optimal gel strength	937:956	optimal gel strength	937:956	The synergistic effect also improved the formation of dense and interpenetrating gel networks, which reduced cooking loss and produced composite MP gels with optimal gel strength.					
37352715	5	44	theme	FTIR	969:972	arg1	spectroscopy					974:985	FTIR spectroscopy	969:985	FTIR spectroscopy	969:985	Moreover, FTIR spectroscopy revealed the presence of electrostatic interactions in the hybrid gel system.					
37352715	5	45	theme	gel	1053:1055	arg1	system					1057:1062	the hybrid gel system	1042:1062	the hybrid gel system	1042:1062	Moreover, FTIR spectroscopy revealed the presence of electrostatic interactions in the hybrid gel system.					
37352715	4	46	theme	interpenetrating	843:858	arg1	networks					864:871	dense and interpenetrating gel networks	833:871	dense and interpenetrating gel networks	833:871	The synergistic effect also improved the formation of dense and interpenetrating gel networks, which reduced cooking loss and produced composite MP gels with optimal gel strength.					
37352715	6	47	theme	components	1189:1198	arg1	use					1151:1153	the effective use	1137:1153	the effective use of high-tech composite functional components to improve the quality of gel products	1137:1237	This study provides a theoretical basis and experimental foundation for the effective use of high-tech composite functional components to improve the quality of gel products.					
37352715	4	48	theme	composite	914:922	arg1	gels					927:930	composite MP gels	914:930	composite MP gels	914:930	The synergistic effect also improved the formation of dense and interpenetrating gel networks, which reduced cooking loss and produced composite MP gels with optimal gel strength.					
37352715	3	49	theme	aggregates	675:684	arg1	formation					662:670	the formation	658:670	the formation of aggregates wrapping hydrophobic groups	658:712	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	3	49	theme	aggregates	675:684	arg1	rearrangement					624:636	the rearrangement	620:636	the rearrangement of the proteins	620:652	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	3	50	theme	secondary	533:541	arg1	structure					543:551	the secondary structure results	529:559	the secondary structure results	529:559	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	1	51	theme	gel	245:247	arg1	quality					208:214	the quality	204:214	the quality of myofibrillar protein (MP) gel	204:247	To improve the limitation of transglutaminase on the quality of myofibrillar protein (MP) gel, this study investigated the synergistic effect of ultrasonic pretreatment in combination with carrageenan on the gel properties of transglutaminase-mediated MP gels.					
37352715	0	52	theme	myofibrillar	80:91	arg1	protein					93:99	transglutaminase-mediated porcine myofibrillar protein	46:99	transglutaminase-mediated porcine myofibrillar protein	46:99	Mechanism for improving the gel properties of transglutaminase-mediated porcine myofibrillar protein by ultrasonic pretreatment combined with carrageenan.					
37352715	6	53	theme	composite	1168:1176	arg1	components					1189:1198	high-tech composite functional components	1158:1198	high-tech composite functional components	1158:1198	This study provides a theoretical basis and experimental foundation for the effective use of high-tech composite functional components to improve the quality of gel products.					
37352715	4	54	theme	networks	864:871	arg1	formation					820:828	the formation	816:828	the formation	816:828	The synergistic effect also improved the formation of dense and interpenetrating gel networks, which reduced cooking loss and produced composite MP gels with optimal gel strength.					
37352715	2	55	theme	lower	458:462	arg1	hydrophobicity					472:485	lower surface hydrophobicity	458:485	lower surface hydrophobicity	458:485	The synergistic effect generated gel with lower surface hydrophobicity and fluorescence intensity.					
37352715	6	56	theme	functional	1178:1187	arg1	components					1189:1198	high-tech composite functional components	1158:1198	high-tech composite functional components	1158:1198	This study provides a theoretical basis and experimental foundation for the effective use of high-tech composite functional components to improve the quality of gel products.					
37352715	5	57	from	presence	1000:1007	arg1	system					1057:1062	the hybrid gel system	1042:1062	the hybrid gel system	1042:1062	Moreover, FTIR spectroscopy revealed the presence of electrostatic interactions in the hybrid gel system.					
37352715	0	58	theme	porcine	72:78	arg1	protein					93:99	transglutaminase-mediated porcine myofibrillar protein	46:99	transglutaminase-mediated porcine myofibrillar protein	46:99	Mechanism for improving the gel properties of transglutaminase-mediated porcine myofibrillar protein by ultrasonic pretreatment combined with carrageenan.					
37352715	4	59	theme	gel	860:862	arg1	networks					864:871	dense and interpenetrating gel networks	833:871	dense and interpenetrating gel networks	833:871	The synergistic effect also improved the formation of dense and interpenetrating gel networks, which reduced cooking loss and produced composite MP gels with optimal gel strength.					
37352715	3	60	dep	caused	613:618	arg1	changed					721:727	changed	721:727	changed the structure and phase behavior of the proteins	721:776	Combined with the secondary structure results, it can be hypothesized that the synergistic effect caused the rearrangement of the proteins and the formation of aggregates wrapping hydrophobic groups, which changed the structure and phase behavior of the proteins.					
37352715	6	61	dep	basis	1099:1103	arg1	theoretical					1087:1097	theoretical	1087:1097	theoretical	1087:1097	This study provides a theoretical basis and experimental foundation for the effective use of high-tech composite functional components to improve the quality of gel products.					
35955952	7	0	theme	contact	1141:1147	arg1	change					1133:1138	hydrophilicity change	1118:1138	hydrophilicity change (contact angle measurements)	1118:1167	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	7	0	theme	contact	1141:1147	arg1	measurements					1155:1166	contact angle measurements	1141:1166	contact angle measurements	1141:1166	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	8	1	theme	carbodiimide	1174:1185	arg1	coupling					1187:1194	The carbodiimide coupling	1170:1194	The carbodiimide coupling	1170:1194	The carbodiimide coupling resulted in higher CS grafting on the PLA surface.					
35955952	5	2	theme	CS	705:706	arg1	samples					716:722	CS grafted samples	705:722	CS grafted samples	705:722	As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation.					
35955952	5	2	theme	CS	705:706	arg1	step					663:666	a next step	656:666	a next step for the combined saccharide coating	656:702	As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation.					
35955952	4	3	theme	PLA	554:556	arg1	surfaces					558:565	Plasma-treated PLA surfaces	539:565	Plasma-treated PLA surfaces	539:565	Plasma-treated PLA surfaces were coated with CS either direct coating method or the carbodiimide coupling method.					
35955952	7	4	theme	angle	1149:1153	arg1	change					1133:1138	hydrophilicity change	1118:1138	hydrophilicity change (contact angle measurements)	1118:1167	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	7	4	theme	angle	1149:1153	arg1	measurements					1155:1166	contact angle measurements	1141:1166	contact angle measurements	1141:1166	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	11	5	theme	CS-ChS	1567:1572	arg1	coating					1574:1580	the CS-ChS coating	1563:1580	the CS-ChS coating	1563:1580	Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.					
35955952	3	6	theme	antibacterial	472:484	arg1	chitosan					413:420	chitosan	413:420	chitosan (CS)	413:425	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	3	6	theme	antibacterial	472:484	arg1	sulfate					443:449	chondroitin sulfate	431:449	chondroitin sulfate (ChS)	431:455	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	3	6	theme	antibacterial	472:484	arg1	materials					494:502	antibacterial coating materials	472:502	antibacterial coating materials on polylactic acid (PLA) surfaces	472:536	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	1	7	theme	biomedical	252:261	arg1	devices					263:269	biomedical devices	252:269	biomedical devices	252:269	More than half of the hospital-associated infections worldwide are related to the adhesion of bacteria cells to biomedical devices and implants.					
35955952	5	8	theme	saccharide	685:694	arg1	coating					696:702	the combined saccharide coating	672:702	the combined saccharide coating	672:702	As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation.					
35955952	3	9	theme	acid	518:521	arg1	surfaces					529:536	polylactic acid (PLA) surfaces	507:536	polylactic acid (PLA) surfaces	507:536	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	11	10	contain	have	1641:1644	arg2	mechanism					1661:1669	a dual killing mechanism	1646:1669	a dual killing mechanism	1646:1669	Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.					
35955952	11	10	contain	have	1641:1644	arg1	films					1635:1639	drug-loaded films	1623:1639	drug-loaded films	1623:1639	Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.					
35955952	7	11	theme	hydrophilicity	1118:1131	arg1	change					1133:1138	hydrophilicity change	1118:1138	hydrophilicity change (contact angle measurements)	1118:1167	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	7	11	theme	hydrophilicity	1118:1131	arg1	measurements					1155:1166	contact angle measurements	1141:1166	contact angle measurements	1141:1166	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	4	12	theme	direct	594:599	arg1	method					609:614	direct coating method	594:614	direct coating method	594:614	Plasma-treated PLA surfaces were coated with CS either direct coating method or the carbodiimide coupling method.					
35955952	7	13	theme	topography	1089:1098	arg1	SEM					1108:1110	SEM	1108:1110	SEM	1108:1110	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	7	13	theme	topography	1089:1098	arg1	images					1100:1105	surface topography images	1081:1105	surface topography images (SEM)	1081:1111	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	10	14	theme	adhered	1443:1449	arg1	amount					1436:1441	the lomefloxacin amount	1419:1441	the lomefloxacin amount adhered to the film coatings	1419:1470	Moreover, these interactions increased the lomefloxacin amount adhered to the film coatings and extended the drug release profile.					
35955952	6	15	theme	coatings	910:917	arg1	loading					860:866	the drug loading and releasing efficiency	851:891	loading	860:866	Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution.					
35955952	6	15	theme	coatings	910:917	arg1	efficiency					882:891	the drug loading and releasing efficiency	851:891	efficiency	882:891	Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution.					
35955952	0	16	theme	Treated	109:115	arg1	Films					133:137	Plasma Treated Polylactic Acid Films	102:137	Plasma Treated Polylactic Acid Films	102:137	Effect of Saccharides Coating on Antibacterial Potential and Drug Loading and Releasing Capability of Plasma Treated Polylactic Acid Films.					
35955952	5	17	theme	complex	793:799	arg1	formation					807:815	the polyelectrolyte complex (PEC) formation	773:815	the polyelectrolyte complex (PEC) formation	773:815	As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation.					
35955952	3	18	from	materials	494:502	arg1	surfaces					529:536	polylactic acid (PLA) surfaces	507:536	polylactic acid (PLA) surfaces	507:536	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	9	19	theme	separate	1361:1368	arg1	coatings					1370:1377	the separate coatings	1357:1377	the separate coatings	1357:1377	The coatings with the PEC formation between CS-ChS showed improved activity against the bacteria strains than the separate coatings.					
35955952	1	20	theme	infections	182:191	arg1	worldwide					193:201	the hospital-associated infections worldwide	158:201	the hospital-associated infections worldwide	158:201	More than half of the hospital-associated infections worldwide are related to the adhesion of bacteria cells to biomedical devices and implants.					
35955952	4	21	theme	carbodiimide	623:634	arg1	method					645:650	the carbodiimide coupling method	619:650	the carbodiimide coupling method	619:650	Plasma-treated PLA surfaces were coated with CS either direct coating method or the carbodiimide coupling method.					
35955952	0	22	theme	Plasma	102:107	arg1	Films					133:137	Plasma Treated Polylactic Acid Films	102:137	Plasma Treated Polylactic Acid Films	102:137	Effect of Saccharides Coating on Antibacterial Potential and Drug Loading and Releasing Capability of Plasma Treated Polylactic Acid Films.					
35955952	11	23	theme	contact	1591:1597	arg1	mechanism					1607:1615	a contact killing mechanism	1589:1615	a contact killing mechanism	1589:1615	Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.					
35955952	6	24	theme	grafted	923:929	arg1	samples					931:937	CS grafted samples	920:937	CS grafted samples	920:937	Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution.					
35955952	10	25	theme	lomefloxacin	1423:1434	arg1	amount					1436:1441	the lomefloxacin amount	1419:1441	the lomefloxacin amount adhered to the film coatings	1419:1470	Moreover, these interactions increased the lomefloxacin amount adhered to the film coatings and extended the drug release profile.					
35955952	3	26	theme	polylactic	507:516	arg1	acid					518:521	polylactic acid	507:521	polylactic acid (PLA) surfaces	507:536	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	3	26	theme	polylactic	507:516	arg1	PLA					524:526	PLA	524:526	PLA	524:526	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	11	27	dep	contact	1687:1693	arg1	killing					1708:1714	killing	1708:1714	killing	1708:1714	Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.					
35955952	1	28	theme	hospital-associated	162:180	arg1	worldwide					193:201	the hospital-associated infections worldwide	158:201	the hospital-associated infections worldwide	158:201	More than half of the hospital-associated infections worldwide are related to the adhesion of bacteria cells to biomedical devices and implants.					
35955952	11	29	theme	killing	1599:1605	arg1	mechanism					1607:1615	a contact killing mechanism	1589:1615	a contact killing mechanism	1589:1615	Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.					
35955952	10	30	theme	release	1494:1500	arg1	profile					1502:1508	the drug release profile	1485:1508	the drug release profile	1485:1508	Moreover, these interactions increased the lomefloxacin amount adhered to the film coatings and extended the drug release profile.					
35955952	2	31	theme	antibacterial	374:386	arg1	property					388:395	the antibacterial property	370:395	the antibacterial property	370:395	To prevent these infections, it is crucial to modify biomaterial surfaces to develop the antibacterial property.					
35955952	6	32	theme	film	905:908	arg1	coatings					910:917	the thin film coatings	896:917	the thin film coatings	896:917	Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution.					
35955952	11	33	theme	killing	1653:1659	arg1	mechanism					1661:1669	a dual killing mechanism	1646:1669	a dual killing mechanism	1646:1669	Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.					
35955952	7	34	theme	elemental	1043:1051	arg1	XPS					1075:1077	XPS	1075:1077	XPS	1075:1077	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	7	34	theme	elemental	1043:1051	arg1	analysis					1065:1072	elemental composition analysis	1043:1072	elemental composition analysis (XPS)	1043:1078	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	1	35	theme	worldwide	193:201	arg1	half					150:153	More than half	140:153	More than half of the hospital-associated infections worldwide	140:201	More than half of the hospital-associated infections worldwide are related to the adhesion of bacteria cells to biomedical devices and implants.					
35955952	6	36	theme	thin	900:903	arg1	coatings					910:917	the thin film coatings	896:917	the thin film coatings	896:917	Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution.					
35955952	0	37	theme	Acid	128:131	arg1	Films					133:137	Plasma Treated Polylactic Acid Films	102:137	Plasma Treated Polylactic Acid Films	102:137	Effect of Saccharides Coating on Antibacterial Potential and Drug Loading and Releasing Capability of Plasma Treated Polylactic Acid Films.					
35955952	8	38	from	grafting	1218:1225	arg1	surface					1238:1244	the PLA surface	1230:1244	the PLA surface	1230:1244	The carbodiimide coupling resulted in higher CS grafting on the PLA surface.					
35955952	4	39	theme	coating	601:607	arg1	method					609:614	direct coating method	594:614	direct coating method	594:614	Plasma-treated PLA surfaces were coated with CS either direct coating method or the carbodiimide coupling method.					
35955952	0	40	theme	Saccharides	10:20	arg1	Effect					0:5	Effect	0:5	Effect of Saccharides	0:20	Effect of Saccharides Coating on Antibacterial Potential and Drug Loading and Releasing Capability of Plasma Treated Polylactic Acid Films.					
35955952	9	41	theme	PEC	1269:1271	arg1	formation					1273:1281	the PEC formation	1265:1281	the PEC formation between CS-ChS	1265:1296	The coatings with the PEC formation between CS-ChS showed improved activity against the bacteria strains than the separate coatings.					
35955952	0	42	theme	Polylactic	117:126	arg1	Films					133:137	Plasma Treated Polylactic Acid Films	102:137	Plasma Treated Polylactic Acid Films	102:137	Effect of Saccharides Coating on Antibacterial Potential and Drug Loading and Releasing Capability of Plasma Treated Polylactic Acid Films.					
35955952	2	43	theme	biomaterial	338:348	arg1	surfaces					350:357	biomaterial surfaces	338:357	biomaterial surfaces	338:357	To prevent these infections, it is crucial to modify biomaterial surfaces to develop the antibacterial property.					
35955952	4	44	theme	Plasma-treated	539:552	arg1	surfaces					558:565	Plasma-treated PLA surfaces	539:565	Plasma-treated PLA surfaces	539:565	Plasma-treated PLA surfaces were coated with CS either direct coating method or the carbodiimide coupling method.					
35955952	1	45	theme	More	140:143	arg1	half					150:153	More than half	140:153	More than half of the hospital-associated infections worldwide	140:201	More than half of the hospital-associated infections worldwide are related to the adhesion of bacteria cells to biomedical devices and implants.					
35955952	0	46	theme	Antibacterial	33:45	arg1	Potential					47:55	Antibacterial Potential	33:55	Antibacterial Potential	33:55	Effect of Saccharides Coating on Antibacterial Potential and Drug Loading and Releasing Capability of Plasma Treated Polylactic Acid Films.					
35955952	8	47	theme	CS	1215:1216	arg1	grafting					1218:1225	higher CS grafting	1208:1225	higher CS grafting on the PLA surface	1208:1244	The carbodiimide coupling resulted in higher CS grafting on the PLA surface.					
35955952	4	48	theme	coupling	636:643	arg1	method					645:650	the carbodiimide coupling method	619:650	the carbodiimide coupling method	619:650	Plasma-treated PLA surfaces were coated with CS either direct coating method or the carbodiimide coupling method.					
35955952	6	49	theme	ChS	982:984	arg1	solution					986:993	lomefloxacin-containing ChS solution	958:993	lomefloxacin-containing ChS solution	958:993	Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution.					
35955952	5	50	theme	ChS	741:743	arg1	solution					745:752	ChS solution	741:752	ChS solution	741:752	As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation.					
35955952	0	51	theme	Films	133:137	arg1	Capability					88:97	Capability	88:97	Capability of Plasma Treated Polylactic Acid Films	88:137	Effect of Saccharides Coating on Antibacterial Potential and Drug Loading and Releasing Capability of Plasma Treated Polylactic Acid Films.					
35955952	6	52	theme	CS	920:921	arg1	samples					931:937	CS grafted samples	920:937	CS grafted samples	920:937	Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution.					
35955952	11	53	dep	have	1641:1644	arg1	includes					1678:1685	includes	1678:1685	includes contact, and release killing	1678:1714	Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.					
35955952	3	54	theme	chondroitin	431:441	arg1	ChS					452:454	ChS	452:454	ChS	452:454	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	3	54	theme	chondroitin	431:441	arg1	sulfate					443:449	chondroitin sulfate	431:449	chondroitin sulfate (ChS)	431:455	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	3	54	theme	chondroitin	431:441	arg1	chitosan					413:420	chitosan	413:420	chitosan (CS)	413:425	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	3	54	theme	chondroitin	431:441	arg1	materials					494:502	antibacterial coating materials	472:502	antibacterial coating materials on polylactic acid (PLA) surfaces	472:536	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	6	55	theme	releasing	872:880	arg1	efficiency					882:891	the drug loading and releasing efficiency	851:891	efficiency	882:891	Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution.					
35955952	6	56	theme	lomefloxacin-containing	958:980	arg1	solution					986:993	lomefloxacin-containing ChS solution	958:993	lomefloxacin-containing ChS solution	958:993	Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution.					
35955952	10	57	theme	drug	1489:1492	arg1	profile					1502:1508	the drug release profile	1485:1508	the drug release profile	1485:1508	Moreover, these interactions increased the lomefloxacin amount adhered to the film coatings and extended the drug release profile.					
35955952	11	58	theme	inhibition	1532:1541	arg1	test					1543:1546	inhibition test	1532:1546	inhibition test	1532:1546	Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.					
35955952	7	59	theme	successful	1000:1009	arg1	modifications					1011:1023	The successful modifications	996:1023	The successful modifications	996:1023	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	5	60	theme	combined	676:683	arg1	coating					696:702	the combined saccharide coating	672:702	the combined saccharide coating	672:702	As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation.					
35955952	7	61	theme	composition	1053:1063	arg1	XPS					1075:1077	XPS	1075:1077	XPS	1075:1077	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	7	61	theme	composition	1053:1063	arg1	analysis					1065:1072	elemental composition analysis	1043:1072	elemental composition analysis (XPS)	1043:1078	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	11	62	theme	drug-loaded	1623:1633	arg1	films					1635:1639	drug-loaded films	1623:1639	drug-loaded films	1623:1639	Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.					
35955952	9	63	theme	improved	1305:1312	arg1	activity					1314:1321	improved activity	1305:1321	improved activity against the bacteria strains than the separate coatings	1305:1377	The coatings with the PEC formation between CS-ChS showed improved activity against the bacteria strains than the separate coatings.					
35955952	10	64	theme	film	1458:1461	arg1	coatings					1463:1470	the film coatings	1454:1470	the film coatings	1454:1470	Moreover, these interactions increased the lomefloxacin amount adhered to the film coatings and extended the drug release profile.					
35955952	7	65	theme	surface	1081:1087	arg1	topography					1089:1098	surface topography	1081:1098	surface topography images (SEM)	1081:1111	The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements).					
35955952	8	66	theme	PLA	1234:1236	arg1	surface					1238:1244	the PLA surface	1230:1244	the PLA surface	1230:1244	The carbodiimide coupling resulted in higher CS grafting on the PLA surface.					
35955952	9	67	with	coatings	1251:1258	arg1	formation					1273:1281	the PEC formation	1265:1281	the PEC formation between CS-ChS	1265:1296	The coatings with the PEC formation between CS-ChS showed improved activity against the bacteria strains than the separate coatings.					
35955952	8	68	theme	higher	1208:1213	arg1	grafting					1218:1225	higher CS grafting	1208:1225	higher CS grafting on the PLA surface	1208:1244	The carbodiimide coupling resulted in higher CS grafting on the PLA surface.					
35955952	5	69	theme	next	658:661	arg1	samples					716:722	CS grafted samples	705:722	CS grafted samples	705:722	As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation.					
35955952	5	69	theme	next	658:661	arg1	step					663:666	a next step	656:666	a next step for the combined saccharide coating	656:702	As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation.					
35955952	11	70	theme	dual	1648:1651	arg1	mechanism					1661:1669	a dual killing mechanism	1646:1669	a dual killing mechanism	1646:1669	Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.					
35955952	5	71	theme	grafted	708:714	arg1	samples					716:722	CS grafted samples	705:722	CS grafted samples	705:722	As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation.					
35955952	5	71	theme	grafted	708:714	arg1	step					663:666	a next step	656:666	a next step for the combined saccharide coating	656:702	As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation.					
35955952	5	72	theme	PEC	802:804	arg1	formation					807:815	the polyelectrolyte complex (PEC) formation	773:815	the polyelectrolyte complex (PEC) formation	773:815	As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation.					
35955952	5	73	theme	polyelectrolyte	777:791	arg1	formation					807:815	the polyelectrolyte complex (PEC) formation	773:815	the polyelectrolyte complex (PEC) formation	773:815	As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation.					
35955952	3	74	theme	coating	486:492	arg1	chitosan					413:420	chitosan	413:420	chitosan (CS)	413:425	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	3	74	theme	coating	486:492	arg1	sulfate					443:449	chondroitin sulfate	431:449	chondroitin sulfate (ChS)	431:455	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	3	74	theme	coating	486:492	arg1	materials					494:502	antibacterial coating materials	472:502	antibacterial coating materials on polylactic acid (PLA) surfaces	472:536	In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces.					
35955952	1	75	theme	bacteria	234:241	arg1	cells					243:247	bacteria cells	234:247	bacteria cells	234:247	More than half of the hospital-associated infections worldwide are related to the adhesion of bacteria cells to biomedical devices and implants.					
35955952	0	76	theme	Drug	61:64	arg1	Loading					66:72	Drug Loading	61:72	Drug Loading	61:72	Effect of Saccharides Coating on Antibacterial Potential and Drug Loading and Releasing Capability of Plasma Treated Polylactic Acid Films.					
35955952	6	77	theme	drug	855:858	arg1	loading					860:866	the drug loading and releasing efficiency	851:891	loading	860:866	Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution.					
35955952	9	78	theme	bacteria	1335:1342	arg1	strains					1344:1350	the bacteria strains	1331:1350	the bacteria strains	1331:1350	The coatings with the PEC formation between CS-ChS showed improved activity against the bacteria strains than the separate coatings.					
35955952	11	79	theme	test	1543:1546	arg1	zone					1524:1527	the zone	1520:1527	the zone of inhibition test	1520:1546	Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.					
35955952	1	80	theme	than	145:148	arg1	half					150:153	More than half	140:153	More than half of the hospital-associated infections worldwide	140:201	More than half of the hospital-associated infections worldwide are related to the adhesion of bacteria cells to biomedical devices and implants.					
35955952	1	81	theme	cells	243:247	arg1	adhesion					222:229	the adhesion	218:229	the adhesion of bacteria cells to biomedical devices and implants	218:282	More than half of the hospital-associated infections worldwide are related to the adhesion of bacteria cells to biomedical devices and implants.					
36768519	10	0	theme	Ag	1660:1661	arg1	particles					1663:1671	the Ag particles	1656:1671	the Ag particles obtained using distilled water	1656:1702	DLS analysis showed that the nanoAg obtained in the plasma water-based starch matrix were smaller than the Ag particles obtained using distilled water.					
36768519	9	1	theme	FTIR-ATR	1382:1389	arg1	spectra					1391:1397	FTIR-ATR spectra	1382:1397	FTIR-ATR spectra	1382:1397	The analysis of FTIR-ATR spectra showed that the type of water used and the synthesis of gold and silver nanoparticles did not lead to changes in the chemical structure of potato starch.					
36768519	10	2	theme	distilled	1688:1696	arg1	water					1698:1702	distilled water	1688:1702	distilled water	1688:1702	DLS analysis showed that the nanoAg obtained in the plasma water-based starch matrix were smaller than the Ag particles obtained using distilled water.					
36768519	3	3	theme	metallic	557:564	arg1	nanoparticles					566:578	the obtained metallic nanoparticles	544:578	the obtained metallic nanoparticles	544:578	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	12	4	theme	potential	2037:2045	arg1	applications					2047:2058	many potential applications	2032:2058	many potential applications	2032:2058	This work shows the possibility of using plasma water in the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications.					
36768519	7	5	theme	Electron	1090:1097	arg1	Microscopy					1099:1108	Scanning Electron Microscopy	1081:1108	Scanning Electron Microscopy	1081:1108	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	7	5	theme	Electron	1090:1097	arg1	SEM					1076:1078	SEM	1076:1078	SEM (Scanning Electron Microscopy) images	1076:1116	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	0	6	theme	Properties	30:39	arg1	Comparison					0:9	Comparison	0:9	Comparison of Physicochemical Properties of Silver and Gold Nanocomposites	0:73	Comparison of Physicochemical Properties of Silver and Gold Nanocomposites Based on Potato Starch in Distilled and Cold Plasma-Treated Water.					
36768519	1	7	theme	science	224:230	arg1	fields					214:219	fields	214:219	fields of science	214:230	Nanometal-containing biocomposites find wide use in many industries and fields of science.					
36768519	1	7	theme	science	224:230	arg1	industries					199:208	many industries	194:208	many industries	194:208	Nanometal-containing biocomposites find wide use in many industries and fields of science.					
36768519	6	8	theme	silver	962:967	arg1	nanoparticles					969:981	gold and silver nanoparticles	953:981	gold and silver nanoparticles	953:981	Studies have shown that gold and silver nanoparticles were successfully obtained in a matrix of potato starch in distilled water and plasma water.					
36768519	6	9	theme	gold	953:956	arg1	nanoparticles					969:981	gold and silver nanoparticles	953:981	gold and silver nanoparticles	953:981	Studies have shown that gold and silver nanoparticles were successfully obtained in a matrix of potato starch in distilled water and plasma water.					
36768519	4	10	theme	starch	732:737	arg1	paste					739:743	a starch paste	730:743	a starch paste made in distilled water and in distilled water exposed to low-temperature, low-pressure plasma	730:838	The metallic nanoparticles were synthesised within a starch paste made in distilled water and in distilled water exposed to low-temperature, low-pressure plasma.					
36768519	3	11	theme	functional	615:624	arg1	properties					626:635	the physicochemical and functional properties	591:635	the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch	526:676	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	6	12	theme	distilled	1042:1050	arg1	water					1052:1056	distilled water	1042:1056	distilled water	1042:1056	Studies have shown that gold and silver nanoparticles were successfully obtained in a matrix of potato starch in distilled water and plasma water.					
36768519	0	13	from	Starch	91:96	arg1	Water					135:139	Distilled and Cold Plasma-Treated Water	101:139	Distilled and Cold Plasma-Treated Water	101:139	Comparison of Physicochemical Properties of Silver and Gold Nanocomposites Based on Potato Starch in Distilled and Cold Plasma-Treated Water.					
36768519	4	14	theme	metallic	683:690	arg1	nanoparticles					692:704	The metallic nanoparticles	679:704	The metallic nanoparticles	679:704	The metallic nanoparticles were synthesised within a starch paste made in distilled water and in distilled water exposed to low-temperature, low-pressure plasma.					
36768519	0	15	theme	Nanocomposites	60:73	arg1	Properties					30:39	Physicochemical Properties	14:39	Physicochemical Properties of Silver and Gold Nanocomposites	14:73	Comparison of Physicochemical Properties of Silver and Gold Nanocomposites Based on Potato Starch in Distilled and Cold Plasma-Treated Water.					
36768519	3	16	theme	physicochemical	595:609	arg1	properties					626:635	the physicochemical and functional properties	591:635	the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch	526:676	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	1	17	from	use	187:189	arg1	fields					214:219	fields	214:219	fields of science	214:230	Nanometal-containing biocomposites find wide use in many industries and fields of science.					
36768519	1	17	from	use	187:189	arg1	industries					199:208	many industries	194:208	many industries	194:208	Nanometal-containing biocomposites find wide use in many industries and fields of science.					
36768519	5	18	theme	physicochemical	901:915	arg1	properties					917:926	their physicochemical properties	895:926	their physicochemical properties	895:926	The materials produced were characterised in terms of their physicochemical properties.					
36768519	9	19	from	changes	1501:1507	arg1	structure					1525:1533	the chemical structure	1512:1533	the chemical structure of potato starch	1512:1550	The analysis of FTIR-ATR spectra showed that the type of water used and the synthesis of gold and silver nanoparticles did not lead to changes in the chemical structure of potato starch.					
36768519	6	20	theme	starch	1032:1037	arg1	matrix					1015:1020	a matrix	1013:1020	a matrix of potato starch in distilled water and plasma water	1013:1073	Studies have shown that gold and silver nanoparticles were successfully obtained in a matrix of potato starch in distilled water and plasma water.					
36768519	12	21	theme	promising	1995:2003	arg1	starch					1971:1976	potato starch	1964:1976	potato starch	1964:1976	This work shows the possibility of using plasma water in the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications.					
36768519	12	21	theme	promising	1995:2003	arg1	polysaccharide					2005:2018	a very promising polysaccharide	1988:2018	a very promising polysaccharide in terms of many potential applications	1988:2058	This work shows the possibility of using plasma water in the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications.					
36768519	1	22	theme	wide	182:185	arg1	use					187:189	wide use	182:189	wide use in many industries and fields of science	182:230	Nanometal-containing biocomposites find wide use in many industries and fields of science.					
36768519	2	23	theme	materials	273:281	arg1	properties					253:262	The physicochemical properties	233:262	The physicochemical properties of these materials	233:281	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	6	24	from	water	1052:1056	arg1	matrix					1015:1020	a matrix	1013:1020	a matrix of potato starch in distilled water and plasma water	1013:1073	Studies have shown that gold and silver nanoparticles were successfully obtained in a matrix of potato starch in distilled water and plasma water.					
36768519	6	25	from	matrix	1015:1020	arg1	water					1069:1073	plasma water	1062:1073	plasma water	1062:1073	Studies have shown that gold and silver nanoparticles were successfully obtained in a matrix of potato starch in distilled water and plasma water.					
36768519	6	25	from	matrix	1015:1020	arg1	water					1052:1056	distilled water	1042:1056	distilled water	1042:1056	Studies have shown that gold and silver nanoparticles were successfully obtained in a matrix of potato starch in distilled water and plasma water.					
36768519	7	26	attach	presence	1151:1158	arg1	composites					1205:1214	the obtained composites	1192:1214	the obtained composites	1192:1214	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	7	26	attach	presence	1151:1158	arg2	nanosilver					1178:1187	nanosilver	1178:1187	nanosilver	1178:1187	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	7	26	attach	presence	1151:1158	arg2	nanosilver					1163:1172	nanosilver	1163:1172	nanosilver	1163:1172	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	4	27	theme	distilled	776:784	arg1	water					786:790	distilled water	776:790	distilled water exposed to low-temperature, low-pressure plasma	776:838	The metallic nanoparticles were synthesised within a starch paste made in distilled water and in distilled water exposed to low-temperature, low-pressure plasma.					
36768519	11	28	theme	dark	1860:1863	arg1	purple					1865:1870	dark purple	1860:1870	dark purple	1860:1870	Colour analysis showed that the nanocomposites without nanometals were colourless, while those containing nanoAg were yellow, while those with nanoAu were dark purple.					
36768519	0	29	theme	Distilled	101:109	arg1	Water					135:139	Distilled and Cold Plasma-Treated Water	101:139	Distilled and Cold Plasma-Treated Water	101:139	Comparison of Physicochemical Properties of Silver and Gold Nanocomposites Based on Potato Starch in Distilled and Cold Plasma-Treated Water.					
36768519	0	30	theme	Silver	44:49	arg1	Nanocomposites					60:73	Silver and Gold Nanocomposites	44:73	Nanocomposites	60:73	Comparison of Physicochemical Properties of Silver and Gold Nanocomposites Based on Potato Starch in Distilled and Cold Plasma-Treated Water.					
36768519	7	31	theme	obtained	1196:1203	arg1	composites					1205:1214	the obtained composites	1192:1214	the obtained composites	1192:1214	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	9	32	theme	chemical	1516:1523	arg1	structure					1525:1533	the chemical structure	1512:1533	the chemical structure of potato starch	1512:1550	The analysis of FTIR-ATR spectra showed that the type of water used and the synthesis of gold and silver nanoparticles did not lead to changes in the chemical structure of potato starch.					
36768519	8	33	dep	images	1245:1250	arg1	the					1220:1222	the	1220:1222	the	1220:1222	On the basis of microscopic images, the size of nanoparticles was estimated in the range from 5 to 20 nm for nanoAg and from 15 to 40 nm for nanoAu.					
36768519	8	33	dep	images	1245:1250	arg1	basis					1224:1228	basis	1224:1228	basis	1224:1228	On the basis of microscopic images, the size of nanoparticles was estimated in the range from 5 to 20 nm for nanoAg and from 15 to 40 nm for nanoAu.					
36768519	4	34	theme	distilled	753:761	arg1	water					763:767	distilled water	753:767	distilled water	753:767	The metallic nanoparticles were synthesised within a starch paste made in distilled water and in distilled water exposed to low-temperature, low-pressure plasma.					
36768519	3	35	theme	work	455:458	arg1	aim					444:446	The aim	440:446	The aim of the work	440:458	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	0	36	theme	Physicochemical	14:28	arg1	Properties					30:39	Physicochemical Properties	14:39	Physicochemical Properties of Silver and Gold Nanocomposites	14:73	Comparison of Physicochemical Properties of Silver and Gold Nanocomposites Based on Potato Starch in Distilled and Cold Plasma-Treated Water.					
36768519	1	37	theme	Nanometal-containing	142:161	arg1	biocomposites					163:175	Nanometal-containing biocomposites	142:175	Nanometal-containing biocomposites	142:175	Nanometal-containing biocomposites find wide use in many industries and fields of science.					
36768519	10	38	theme	water-based	1612:1622	arg1	matrix					1631:1636	the plasma water-based starch matrix	1601:1636	the plasma water-based starch matrix	1601:1636	DLS analysis showed that the nanoAg obtained in the plasma water-based starch matrix were smaller than the Ag particles obtained using distilled water.					
36768519	12	39	from	water	1921:1925	arg1	synthesis					1934:1942	the synthesis	1930:1942	the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications	1930:2058	This work shows the possibility of using plasma water in the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications.					
36768519	9	40	theme	starch	1545:1550	arg1	structure					1525:1533	the chemical structure	1512:1533	the chemical structure of potato starch	1512:1550	The analysis of FTIR-ATR spectra showed that the type of water used and the synthesis of gold and silver nanoparticles did not lead to changes in the chemical structure of potato starch.					
36768519	11	41	contain	containing	1800:1809	arg2	nanoAg					1811:1816	nanoAg	1811:1816	nanoAg	1811:1816	Colour analysis showed that the nanocomposites without nanometals were colourless, while those containing nanoAg were yellow, while those with nanoAu were dark purple.					
36768519	11	41	contain	containing	1800:1809	arg1	those					1794:1798	those	1794:1798	those	1794:1798	Colour analysis showed that the nanocomposites without nanometals were colourless, while those containing nanoAg were yellow, while those with nanoAu were dark purple.					
36768519	9	42	theme	nanoparticles	1471:1483	arg1	type					1415:1418	the type	1411:1418	the type of water used	1411:1432	The analysis of FTIR-ATR spectra showed that the type of water used and the synthesis of gold and silver nanoparticles did not lead to changes in the chemical structure of potato starch.					
36768519	9	42	theme	nanoparticles	1471:1483	arg1	synthesis					1442:1450	the synthesis	1438:1450	the synthesis of gold and silver nanoparticles	1438:1483	The analysis of FTIR-ATR spectra showed that the type of water used and the synthesis of gold and silver nanoparticles did not lead to changes in the chemical structure of potato starch.					
36768519	3	43	theme	potato	664:669	arg1	starch					671:676	potato starch	664:676	potato starch	664:676	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	4	44	theme	low-temperature	803:817	arg1	plasma					833:838	low-temperature, low-pressure plasma	803:838	low-temperature, low-pressure plasma	803:838	The metallic nanoparticles were synthesised within a starch paste made in distilled water and in distilled water exposed to low-temperature, low-pressure plasma.					
36768519	12	45	theme	potato	1964:1969	arg1	starch					1971:1976	potato starch	1964:1976	potato starch	1964:1976	This work shows the possibility of using plasma water in the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications.					
36768519	12	45	theme	potato	1964:1969	arg1	polysaccharide					2005:2018	a very promising polysaccharide	1988:2018	a very promising polysaccharide in terms of many potential applications	1988:2058	This work shows the possibility of using plasma water in the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications.					
36768519	2	46	theme	metallic	349:356	arg1	nanoparticles					358:370	the metallic nanoparticles	345:370	the metallic nanoparticles	345:370	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	8	47	from	nm	1351:1352	arg1	range					1300:1304	the range	1296:1304	the range from 5 to 20 nm for nanoAg and from 15 to 40 nm for nanoAu	1296:1363	On the basis of microscopic images, the size of nanoparticles was estimated in the range from 5 to 20 nm for nanoAg and from 15 to 40 nm for nanoAu.					
36768519	7	48	theme	UV-Vis	1122:1127	arg1	spectra					1129:1135	UV-Vis spectra	1122:1135	UV-Vis spectra	1122:1135	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	3	49	from	effect	492:497	arg1	properties					626:635	the physicochemical and functional properties	591:635	the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch	526:676	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	3	49	from	effect	492:497	arg1	properties					530:539	the properties	526:539	the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch	526:676	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	9	50	theme	spectra	1391:1397	arg1	analysis					1370:1377	The analysis	1366:1377	The analysis of FTIR-ATR spectra	1366:1397	The analysis of FTIR-ATR spectra showed that the type of water used and the synthesis of gold and silver nanoparticles did not lead to changes in the chemical structure of potato starch.					
36768519	3	51	theme	water	517:521	arg1	effect					492:497	the effect	488:497	the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch	488:676	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	1	52	theme	many	194:197	arg1	industries					199:208	many industries	194:208	many industries	194:208	Nanometal-containing biocomposites find wide use in many industries and fields of science.					
36768519	4	53	dep	low-temperature	803:817	arg1	low-pressure					820:831	low-pressure	820:831	low-pressure	820:831	The metallic nanoparticles were synthesised within a starch paste made in distilled water and in distilled water exposed to low-temperature, low-pressure plasma.					
36768519	12	54	theme	nanometals	1947:1956	arg1	synthesis					1934:1942	the synthesis	1930:1942	the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications	1930:2058	This work shows the possibility of using plasma water in the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications.					
36768519	10	55	theme	DLS	1553:1555	arg1	analysis					1557:1564	DLS analysis	1553:1564	DLS analysis	1553:1564	DLS analysis showed that the nanoAg obtained in the plasma water-based starch matrix were smaller than the Ag particles obtained using distilled water.					
36768519	3	56	theme	obtained	548:555	arg1	nanoparticles					566:578	the obtained metallic nanoparticles	544:578	the obtained metallic nanoparticles	544:578	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	8	57	dep	40	1348:1349	arg1	to					1345:1346	to	1345:1346	to	1345:1346	On the basis of microscopic images, the size of nanoparticles was estimated in the range from 5 to 20 nm for nanoAg and from 15 to 40 nm for nanoAu.					
36768519	12	58	theme	plasma	1914:1919	arg1	water					1921:1925	plasma water	1914:1925	plasma water in the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications	1914:2058	This work shows the possibility of using plasma water in the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications.					
36768519	0	59	theme	Plasma-Treated	120:133	arg1	Water					135:139	Distilled and Cold Plasma-Treated Water	101:139	Distilled and Cold Plasma-Treated Water	101:139	Comparison of Physicochemical Properties of Silver and Gold Nanocomposites Based on Potato Starch in Distilled and Cold Plasma-Treated Water.					
36768519	6	60	from	water	1069:1073	arg1	matrix					1015:1020	a matrix	1013:1020	a matrix of potato starch in distilled water and plasma water	1013:1073	Studies have shown that gold and silver nanoparticles were successfully obtained in a matrix of potato starch in distilled water and plasma water.					
36768519	2	61	theme	polymer	314:320	arg1	character					297:305	the character	293:305	the character of the polymer	293:320	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	2	61	theme	polymer	314:320	arg1	interactions					381:392	the interactions	377:392	the interactions between the biopolymer	377:415	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	2	61	theme	polymer	314:320	arg1	nanoparticles					425:437	the nanoparticles	421:437	the nanoparticles	421:437	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	2	61	theme	polymer	314:320	arg1	size					327:330	size	327:330	size	327:330	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	2	61	theme	polymer	314:320	arg1	shape					336:340	shape	336:340	shape	336:340	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	9	62	theme	water	1423:1427	arg1	type					1415:1418	the type	1411:1418	the type of water used	1411:1432	The analysis of FTIR-ATR spectra showed that the type of water used and the synthesis of gold and silver nanoparticles did not lead to changes in the chemical structure of potato starch.					
36768519	9	62	theme	water	1423:1427	arg1	synthesis					1442:1450	the synthesis	1438:1450	the synthesis of gold and silver nanoparticles	1438:1483	The analysis of FTIR-ATR spectra showed that the type of water used and the synthesis of gold and silver nanoparticles did not lead to changes in the chemical structure of potato starch.					
36768519	8	63	theme	nanoparticles	1265:1277	arg1	size					1257:1260	the size	1253:1260	the size of nanoparticles	1253:1277	On the basis of microscopic images, the size of nanoparticles was estimated in the range from 5 to 20 nm for nanoAg and from 15 to 40 nm for nanoAu.					
36768519	7	64	theme	SEM	1076:1078	arg1	images					1111:1116	SEM (Scanning Electron Microscopy) images	1076:1116	SEM (Scanning Electron Microscopy) images	1076:1116	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	12	65	theme	applications	2047:2058	arg1	terms					2023:2027	terms	2023:2027	terms of many potential applications	2023:2058	This work shows the possibility of using plasma water in the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications.					
36768519	3	66	theme	nanoparticles	566:578	arg1	properties					626:635	the physicochemical and functional properties	591:635	the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch	526:676	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	3	66	theme	nanoparticles	566:578	arg1	properties					530:539	the properties	526:539	the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch	526:676	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	8	67	theme	microscopic	1233:1243	arg1	images					1245:1250	microscopic images	1233:1250	microscopic images	1233:1250	On the basis of microscopic images, the size of nanoparticles was estimated in the range from 5 to 20 nm for nanoAg and from 15 to 40 nm for nanoAu.					
36768519	7	68	theme	Scanning	1081:1088	arg1	Microscopy					1099:1108	Scanning Electron Microscopy	1081:1108	Scanning Electron Microscopy	1081:1108	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	7	68	theme	Scanning	1081:1088	arg1	SEM					1076:1078	SEM	1076:1078	SEM (Scanning Electron Microscopy) images	1076:1116	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	12	69	from	polysaccharide	2005:2018	arg1	terms					2023:2027	terms	2023:2027	terms of many potential applications	2023:2058	This work shows the possibility of using plasma water in the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications.					
36768519	9	70	theme	gold	1455:1458	arg1	nanoparticles					1471:1483	gold and silver nanoparticles	1455:1483	gold and silver nanoparticles	1455:1483	The analysis of FTIR-ATR spectra showed that the type of water used and the synthesis of gold and silver nanoparticles did not lead to changes in the chemical structure of potato starch.					
36768519	11	71	theme	Colour	1705:1710	arg1	analysis					1712:1719	Colour analysis	1705:1719	Colour analysis	1705:1719	Colour analysis showed that the nanocomposites without nanometals were colourless, while those containing nanoAg were yellow, while those with nanoAu were dark purple.					
36768519	9	72	theme	silver	1464:1469	arg1	nanoparticles					1471:1483	gold and silver nanoparticles	1455:1483	gold and silver nanoparticles	1455:1483	The analysis of FTIR-ATR spectra showed that the type of water used and the synthesis of gold and silver nanoparticles did not lead to changes in the chemical structure of potato starch.					
36768519	7	73	from	presence	1151:1158	arg1	composites					1205:1214	the obtained composites	1192:1214	the obtained composites	1192:1214	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	0	74	theme	Potato	84:89	arg1	Starch					91:96	Potato Starch	84:96	Potato Starch in Distilled and Cold Plasma-Treated Water	84:139	Comparison of Physicochemical Properties of Silver and Gold Nanocomposites Based on Potato Starch in Distilled and Cold Plasma-Treated Water.					
36768519	5	75	theme	properties	917:926	arg1	terms					886:890	terms	886:890	terms of their physicochemical properties	886:926	The materials produced were characterised in terms of their physicochemical properties.					
36768519	6	76	theme	potato	1025:1030	arg1	starch					1032:1037	potato starch	1025:1037	potato starch in distilled water and plasma water	1025:1073	Studies have shown that gold and silver nanoparticles were successfully obtained in a matrix of potato starch in distilled water and plasma water.					
36768519	0	77	theme	Cold	115:118	arg1	Water					135:139	Distilled and Cold Plasma-Treated Water	101:139	Distilled and Cold Plasma-Treated Water	101:139	Comparison of Physicochemical Properties of Silver and Gold Nanocomposites Based on Potato Starch in Distilled and Cold Plasma-Treated Water.					
36768519	6	78	from	starch	1032:1037	arg1	water					1069:1073	plasma water	1062:1073	plasma water	1062:1073	Studies have shown that gold and silver nanoparticles were successfully obtained in a matrix of potato starch in distilled water and plasma water.					
36768519	6	78	from	starch	1032:1037	arg1	water					1052:1056	distilled water	1042:1056	distilled water	1042:1056	Studies have shown that gold and silver nanoparticles were successfully obtained in a matrix of potato starch in distilled water and plasma water.					
36768519	12	79	theme	many	2032:2035	arg1	applications					2047:2058	many potential applications	2032:2058	many potential applications	2032:2058	This work shows the possibility of using plasma water in the synthesis of nanometals using potato starch, which is a very promising polysaccharide in terms of many potential applications.					
36768519	8	80	from	nm	1319:1320	arg1	range					1300:1304	the range	1296:1304	the range from 5 to 20 nm for nanoAg and from 15 to 40 nm for nanoAu	1296:1363	On the basis of microscopic images, the size of nanoparticles was estimated in the range from 5 to 20 nm for nanoAg and from 15 to 40 nm for nanoAu.					
36768519	2	81	dep	size	327:330	arg1	the					323:325	the	323:325	the	323:325	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	7	82	theme	nanosilver	1178:1187	arg1	presence					1151:1158	the presence	1147:1158	the presence of nanosilver and nanosilver in the obtained composites	1147:1214	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	3	83	theme	nanocomposites	640:653	arg1	properties					626:635	the physicochemical and functional properties	591:635	the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch	526:676	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	3	83	theme	nanocomposites	640:653	arg1	properties					530:539	the properties	526:539	the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch	526:676	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36768519	10	84	theme	starch	1624:1629	arg1	matrix					1631:1636	the plasma water-based starch matrix	1601:1636	the plasma water-based starch matrix	1601:1636	DLS analysis showed that the nanoAg obtained in the plasma water-based starch matrix were smaller than the Ag particles obtained using distilled water.					
36768519	7	85	theme	nanosilver	1163:1172	arg1	presence					1151:1158	the presence	1147:1158	the presence of nanosilver and nanosilver in the obtained composites	1147:1214	SEM (Scanning Electron Microscopy) images and UV-Vis spectra confirmed the presence of nanosilver and nanosilver in the obtained composites.					
36768519	9	86	theme	potato	1538:1543	arg1	starch					1545:1550	potato starch	1538:1550	potato starch	1538:1550	The analysis of FTIR-ATR spectra showed that the type of water used and the synthesis of gold and silver nanoparticles did not lead to changes in the chemical structure of potato starch.					
36768519	6	87	theme	plasma	1062:1067	arg1	water					1069:1073	plasma water	1062:1073	plasma water	1062:1073	Studies have shown that gold and silver nanoparticles were successfully obtained in a matrix of potato starch in distilled water and plasma water.					
36768519	10	88	theme	plasma	1605:1610	arg1	matrix					1631:1636	the plasma water-based starch matrix	1601:1636	the plasma water-based starch matrix	1601:1636	DLS analysis showed that the nanoAg obtained in the plasma water-based starch matrix were smaller than the Ag particles obtained using distilled water.					
36768519	0	89	theme	Gold	55:58	arg1	Nanocomposites					60:73	Silver and Gold Nanocomposites	44:73	Nanocomposites	60:73	Comparison of Physicochemical Properties of Silver and Gold Nanocomposites Based on Potato Starch in Distilled and Cold Plasma-Treated Water.					
36768519	8	90	dep	20	1316:1317	arg1	to					1313:1314	to	1313:1314	to	1313:1314	On the basis of microscopic images, the size of nanoparticles was estimated in the range from 5 to 20 nm for nanoAg and from 15 to 40 nm for nanoAu.					
36768519	2	91	theme	physicochemical	237:251	arg1	properties					253:262	The physicochemical properties	233:262	The physicochemical properties of these materials	233:281	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	2	92	theme	nanoparticles	358:370	arg1	character					297:305	the character	293:305	the character of the polymer	293:320	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	2	92	theme	nanoparticles	358:370	arg1	interactions					381:392	the interactions	377:392	the interactions between the biopolymer	377:415	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	2	92	theme	nanoparticles	358:370	arg1	nanoparticles					425:437	the nanoparticles	421:437	the nanoparticles	421:437	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	2	92	theme	nanoparticles	358:370	arg1	size					327:330	size	327:330	size	327:330	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	2	92	theme	nanoparticles	358:370	arg1	shape					336:340	shape	336:340	shape	336:340	The physicochemical properties of these materials depend on the character of the polymer, the size and shape of the metallic nanoparticles, and the interactions between the biopolymer and the nanoparticles.					
36768519	3	93	theme	plasma-treated	502:515	arg1	water					517:521	plasma-treated water	502:521	plasma-treated water	502:521	The aim of the work was to synthesise and study the effect of plasma-treated water on the properties of the obtained metallic nanoparticles as well as the physicochemical and functional properties of nanocomposites based on potato starch.					
36095317	10	0	theme	%	2357:2357	arg1	use					2348:2350	the use	2344:2350	the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively	2344:2461	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	3	1	theme	gels	715:718	arg1	use					668:670	the use	664:670	the use of higher- and lower-dextran-concentration gels	664:718	In case of the heavy-chain fragment, elevated borate concentrations resulted in decreasing tailing and increasing fronting with the use of higher- and lower-dextran-concentration gels, respectively.					
36095317	8	2	from	SDS-CGE	1651:1657	arg1	result					1724:1729	the result	1720:1729	the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1720:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	2	from	SDS-CGE	1651:1657	arg1	shapes					1604:1609	the observed fronting and/or tailing peak shapes	1562:1609	the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations	1562:1697	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	3	theme	matrix	1784:1789	arg1	size					1796:1799	sieving matrix pore size	1776:1799	sieving matrix pore size	1776:1799	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	4	theme	multiple	1734:1741	arg1	changes					1761:1767	changes	1761:1767	changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1761:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	4	theme	multiple	1734:1741	arg1	effects					1743:1749	multiple effects	1734:1749	multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1734:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	9	5	theme	function	2237:2244	arg1	consideration					2198:2210	special consideration	2190:2210	special consideration of its gel cross-linking function	2190:2244	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	8	6	from	fragments	1638:1646	arg1	SDS-CGE					1651:1657	SDS-CGE	1651:1657	SDS-CGE	1651:1657	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	12	7	theme	gel	2819:2821	arg1	electrophoresis					2823:2837	capillary pore-size-gradient gel electrophoresis	2790:2837	capillary pore-size-gradient gel electrophoresis	2790:2837	This novel approach resulted in close to optimal peak shapes for the distantly migrating IgG subunits within a single run, as well as unraveled the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis.					
36095317	6	8	dep	constituents	1213:1224	arg1	migrating					1227:1235	migrating	1227:1235	migrating slower than that of the unconjugated tetrahydroxyborate	1227:1291	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	12	9	theme	single	2712:2717	arg1	run					2719:2721	a single run	2710:2721	a single run	2710:2721	This novel approach resulted in close to optimal peak shapes for the distantly migrating IgG subunits within a single run, as well as unraveled the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis.					
36095317	1	10	theme	monoclonal	192:201	arg1	antibody					203:210	the therapeutic monoclonal antibody	176:210	the therapeutic monoclonal antibody omalizumab	176:221	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	10	11	theme	mM	2371:2372	arg1	acid					2380:2383	dextran/340 mM boric acid	2359:2383	10% dextran/340 mM boric acid	2355:2383	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	7	12	theme	resultant	1492:1500	arg1	mobility					1512:1519	the resultant effective mobility	1488:1519	the resultant effective mobility of the co-ionic species	1488:1543	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	9	13	theme	gel	2219:2221	arg1	function					2237:2244	its gel cross-linking function	2215:2244	its gel cross-linking function	2215:2244	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	3	14	theme	elevated	573:580	arg1	concentrations					589:602	elevated borate concentrations	573:602	elevated borate concentrations	573:602	In case of the heavy-chain fragment, elevated borate concentrations resulted in decreasing tailing and increasing fronting with the use of higher- and lower-dextran-concentration gels, respectively.					
36095317	1	15	theme	omalizumab	212:221	arg1	peaks					167:171	the light- and heavy-chain subunit peaks	132:171	the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab	132:221	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	10	16	theme	dextran/640	2393:2403	arg1	mM					2405:2406	mM	2405:2406	mM	2405:2406	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	5	17	theme	same	980:983	arg1	effect					985:990	the same effect	976:990	the same effect	976:990	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	5	17	theme	same	980:983	arg1	concentration					1017:1029	the increasing borate concentration	995:1029	the increasing borate concentration in both dextran concentrations	995:1060	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	8	18	theme	mobility	1821:1828	arg1	variation					1830:1838	the mobility variation	1817:1838	the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1817:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	1	19	theme	light-	136:141	arg1	peaks					167:171	the light- and heavy-chain subunit peaks	132:171	the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab	132:221	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	12	20	theme	capillary	2790:2798	arg1	electrophoresis					2823:2837	capillary pore-size-gradient gel electrophoresis	2790:2837	capillary pore-size-gradient gel electrophoresis	2790:2837	This novel approach resulted in close to optimal peak shapes for the distantly migrating IgG subunits within a single run, as well as unraveled the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis.					
36095317	7	21	theme	further	1468:1474	arg1	decrease					1476:1483	further decrease	1468:1483	further decrease in the resultant effective mobility of the co-ionic species	1468:1543	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	9	22	theme	species	2045:2051	arg1	mobility					2020:2027	the effective mobility	2006:2027	the effective mobility of the co-ionic species	2006:2051	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	1	23	theme	sodium	243:248	arg1	SDS-CGE					297:303	SDS-CGE	297:303	SDS-CGE	297:303	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	1	23	theme	sodium	243:248	arg1	electrophoresis					280:294	sodium dodecyl sulfate capillary gel electrophoresis	243:294	sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE)	243:304	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	10	24	dep	%	2357:2357	arg1	acid					2380:2383	dextran/340 mM boric acid	2359:2383	10% dextran/340 mM boric acid	2355:2383	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	10	25	theme	gel-buffer	2430:2439	arg1	systems					2441:2447	boric acid-containing gel-buffer systems	2408:2447	10% dextran/640 mM boric acid-containing gel-buffer systems	2389:2447	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	10	26	theme	heavy-chain	2266:2276	arg1	fragments					2278:2286	the light- and heavy-chain fragments	2251:2286	the light- and heavy-chain fragments of the IgG analyte	2251:2305	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	4	27	theme	light-chain	739:749	arg1	fragment					751:758	The light-chain fragment	735:758	The light-chain fragment	735:758	The light-chain fragment, on the other hand, exhibited increased fronting with increasing borate concentration for both dextran concentrations examined in this study.					
36095317	8	28	theme	co-ionic	1847:1854	arg1	adducts					1863:1869	the co-ionic borate adducts	1843:1869	the co-ionic borate adducts with the gel-buffer ingredients	1843:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	5	29	theme	gel-buffer	951:960	arg1	system					962:967	the gel-buffer system	947:967	the gel-buffer system	947:967	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	10	30	theme	boric	2408:2412	arg1	systems					2441:2447	boric acid-containing gel-buffer systems	2408:2447	10% dextran/640 mM boric acid-containing gel-buffer systems	2389:2447	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	10	31	theme	light-	2255:2260	arg1	fragments					2278:2286	the light- and heavy-chain fragments	2251:2286	the light- and heavy-chain fragments of the IgG analyte	2251:2305	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	8	32	theme	adducts	1863:1869	arg1	variation					1830:1838	the mobility variation	1817:1838	the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1817:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	32	theme	adducts	1863:1869	arg1	viscosity					1802:1810	viscosity	1802:1810	viscosity	1802:1810	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	32	theme	adducts	1863:1869	arg1	size					1796:1799	sieving matrix pore size	1776:1799	sieving matrix pore size	1776:1799	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	32	theme	adducts	1863:1869	arg1	pH					1772:1773	pH	1772:1773	pH	1772:1773	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	1	33	theme	heavy-chain	147:157	arg1	peaks					167:171	the light- and heavy-chain subunit peaks	132:171	the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab	132:221	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	6	34	theme	detected	1067:1074	arg1	result					1126:1131	the result	1122:1131	the result of the formation of monomeric and dimeric glycerol-borate complexes	1122:1199	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	6	34	theme	detected	1067:1074	arg1	dispersion					1093:1102	The detected electromigration dispersion	1063:1102	The detected electromigration dispersion	1063:1102	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	6	34	theme	detected	1067:1074	arg1	constituents					1213:1224	co-ionic constituents	1204:1224	co-ionic constituents	1204:1224	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	8	35	theme	observed	1566:1573	arg1	result					1724:1729	the result	1720:1729	the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1720:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	35	theme	observed	1566:1573	arg1	shapes					1604:1609	the observed fronting and/or tailing peak shapes	1562:1609	the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations	1562:1697	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	6	36	theme	unconjugated	1261:1272	arg1	tetrahydroxyborate					1274:1291	the unconjugated tetrahydroxyborate	1257:1291	the unconjugated tetrahydroxyborate	1257:1291	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	1	37	theme	peaks	167:171	arg1	dispersion					118:127	The electromigration dispersion	97:127	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab	97:221	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	5	38	theme	ingredient	927:936	arg1	level					938:942	the glycerol ingredient level	914:942	the glycerol ingredient level	914:942	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	2	39	theme	-dextran-concentration	476:497	arg1	gels					499:502	-dextran-concentration gels	476:502	-dextran-concentration gels	476:502	Increasing boric acid content (340-640 mM) caused electromigration dispersion shifts for both low (2%)- and high (10%)-dextran-concentration gels in all gel-buffer compositions.					
36095317	12	40	theme	long-sought	2749:2759	arg1	solution					2770:2777	the long-sought possible solution	2745:2777	the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis	2745:2837	This novel approach resulted in close to optimal peak shapes for the distantly migrating IgG subunits within a single run, as well as unraveled the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis.					
36095317	0	41	theme	Proteins	87:94	arg1	Electrophoresis					68:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis	31:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins	31:94	Electromigration Dispersion in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins.					
36095317	9	42	link	cross-linked	2109:2120	arg1	gels					2130:2133	borate cross-linked dextran gels	2102:2133	borate cross-linked dextran gels	2102:2133	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	8	43	theme	peak	1599:1602	arg1	result					1724:1729	the result	1720:1729	the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1720:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	43	theme	peak	1599:1602	arg1	shapes					1604:1609	the observed fronting and/or tailing peak shapes	1562:1609	the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations	1562:1697	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	10	44	theme	peak	2313:2316	arg1	shapes					2318:2323	best peak shapes	2308:2323	best peak shapes	2308:2323	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	11	45	theme	borate-gradient-mediated	2524:2547	arg1	mobility					2559:2566	borate-gradient-mediated transient mobility	2524:2566	borate-gradient-mediated transient mobility matching in SDS-CGE of proteins	2524:2598	Based on this observation, here we introduce the concept of borate-gradient-mediated transient mobility matching in SDS-CGE of proteins.					
36095317	5	46	theme	increasing	999:1008	arg1	effect					985:990	the same effect	976:990	the same effect	976:990	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	5	46	theme	increasing	999:1008	arg1	concentration					1017:1029	the increasing borate concentration	995:1029	the increasing borate concentration in both dextran concentrations	995:1060	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	2	47	theme	gel-buffer	511:520	arg1	compositions					522:533	all gel-buffer compositions	507:533	all gel-buffer compositions	507:533	Increasing boric acid content (340-640 mM) caused electromigration dispersion shifts for both low (2%)- and high (10%)-dextran-concentration gels in all gel-buffer compositions.					
36095317	9	48	theme	cross-linked	2109:2120	arg1	gels					2130:2133	borate cross-linked dextran gels	2102:2133	borate cross-linked dextran gels	2102:2133	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	10	49	theme	best	2308:2311	arg1	shapes					2318:2323	best peak shapes	2308:2323	best peak shapes	2308:2323	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	8	50	theme	monoclonal	1618:1627	arg1	fragments					1638:1646	the monoclonal antibody fragments	1614:1646	the monoclonal antibody fragments in SDS-CGE	1614:1657	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	12	51	theme	peak	2650:2653	arg1	shapes					2655:2660	optimal peak shapes	2642:2660	optimal peak shapes for the distantly migrating IgG	2642:2692	This novel approach resulted in close to optimal peak shapes for the distantly migrating IgG subunits within a single run, as well as unraveled the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis.					
36095317	2	52	theme	dispersion	425:434	arg1	shifts					436:441	electromigration dispersion shifts	408:441	electromigration dispersion shifts for both low (2%)- and high (10%)-dextran-concentration gels	408:502	Increasing boric acid content (340-640 mM) caused electromigration dispersion shifts for both low (2%)- and high (10%)-dextran-concentration gels in all gel-buffer compositions.					
36095317	1	53	theme	electromigration	101:116	arg1	dispersion					118:127	The electromigration dispersion	97:127	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab	97:221	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	8	54	theme	fragments	1638:1646	arg1	result					1724:1729	the result	1720:1729	the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1720:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	54	theme	fragments	1638:1646	arg1	shapes					1604:1609	the observed fronting and/or tailing peak shapes	1562:1609	the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations	1562:1697	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	9	55	theme	interest	2081:2088	arg1	molecules					2068:2076	the analyte molecules	2056:2076	the analyte molecules of interest	2056:2088	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	1	56	theme	cross-linked	319:330	arg1	matrices					348:355	borate cross-linked dextran sieving matrices	312:355	borate cross-linked dextran sieving matrices	312:355	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	7	57	theme	dextran	1392:1398	arg1	polymer					1400:1406	the dextran polymer	1388:1406	the dextran polymer	1388:1406	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	9	58	theme	electromigration	1910:1925	arg1	broadening					1952:1961	electromigration dispersion-mediated band broadening	1910:1961	electromigration dispersion-mediated band broadening	1910:1961	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	2	59	dep	low	452:454	arg1	gels					499:502	-dextran-concentration gels	476:502	-dextran-concentration gels	476:502	Increasing boric acid content (340-640 mM) caused electromigration dispersion shifts for both low (2%)- and high (10%)-dextran-concentration gels in all gel-buffer compositions.					
36095317	0	60	theme	Dodecyl	38:44	arg1	Electrophoresis					68:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis	31:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins	31:94	Electromigration Dispersion in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins.					
36095317	1	61	theme	sieving	340:346	arg1	matrices					348:355	borate cross-linked dextran sieving matrices	312:355	borate cross-linked dextran sieving matrices	312:355	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	9	62	theme	band	1947:1950	arg1	broadening					1952:1961	electromigration dispersion-mediated band broadening	1910:1961	electromigration dispersion-mediated band broadening	1910:1961	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	8	63	theme	increasing	1662:1671	arg1	concentrations					1684:1697	increasing boric acid concentrations	1662:1697	increasing boric acid concentrations	1662:1697	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	9	64	theme	acid	2162:2165	arg1	concentration					2167:2179	the boric acid concentration	2152:2179	the boric acid concentration	2152:2179	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	0	65	theme	Capillary	54:62	arg1	Electrophoresis					68:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis	31:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins	31:94	Electromigration Dispersion in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins.					
36095317	12	66	theme	pore-size-gradient	2800:2817	arg1	electrophoresis					2823:2837	capillary pore-size-gradient gel electrophoresis	2790:2837	capillary pore-size-gradient gel electrophoresis	2790:2837	This novel approach resulted in close to optimal peak shapes for the distantly migrating IgG subunits within a single run, as well as unraveled the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis.					
36095317	6	67	theme	complexes	1191:1199	arg1	formation					1140:1148	the formation	1136:1148	the formation of monomeric and dimeric glycerol-borate complexes	1136:1199	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	8	68	theme	acid	1679:1682	arg1	concentrations					1684:1697	increasing boric acid concentrations	1662:1697	increasing boric acid concentrations	1662:1697	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	3	69	theme	lower-dextran-concentration	687:713	arg1	gels					715:718	higher- and lower-dextran-concentration gels	675:718	higher- and lower-dextran-concentration gels	675:718	In case of the heavy-chain fragment, elevated borate concentrations resulted in decreasing tailing and increasing fronting with the use of higher- and lower-dextran-concentration gels, respectively.					
36095317	2	70	theme	acid	375:378	arg1	content					380:386	boric acid content	369:386	boric acid content (340-640 mM)	369:399	Increasing boric acid content (340-640 mM) caused electromigration dispersion shifts for both low (2%)- and high (10%)-dextran-concentration gels in all gel-buffer compositions.					
36095317	2	70	theme	acid	375:378	arg1	mM					397:398	340-640 mM	389:398	340-640 mM	389:398	Increasing boric acid content (340-640 mM) caused electromigration dispersion shifts for both low (2%)- and high (10%)-dextran-concentration gels in all gel-buffer compositions.					
36095317	8	71	theme	effects	1743:1749	arg1	result					1724:1729	the result	1720:1729	the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1720:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	71	theme	effects	1743:1749	arg1	shapes					1604:1609	the observed fronting and/or tailing peak shapes	1562:1609	the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations	1562:1697	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	1	72	theme	dodecyl	250:256	arg1	SDS-CGE					297:303	SDS-CGE	297:303	SDS-CGE	297:303	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	1	72	theme	dodecyl	250:256	arg1	electrophoresis					280:294	sodium dodecyl sulfate capillary gel electrophoresis	243:294	sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE)	243:304	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	6	73	theme	dimeric	1167:1173	arg1	complexes					1191:1199	monomeric and dimeric glycerol-borate complexes	1153:1199	monomeric and dimeric glycerol-borate complexes	1153:1199	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	12	74	theme	optimal	2642:2648	arg1	shapes					2655:2660	optimal peak shapes	2642:2660	optimal peak shapes for the distantly migrating IgG	2642:2692	This novel approach resulted in close to optimal peak shapes for the distantly migrating IgG subunits within a single run, as well as unraveled the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis.					
36095317	3	75	theme	higher-	675:681	arg1	gels					715:718	higher- and lower-dextran-concentration gels	675:718	higher- and lower-dextran-concentration gels	675:718	In case of the heavy-chain fragment, elevated borate concentrations resulted in decreasing tailing and increasing fronting with the use of higher- and lower-dextran-concentration gels, respectively.					
36095317	1	76	theme	capillary	266:274	arg1	SDS-CGE					297:303	SDS-CGE	297:303	SDS-CGE	297:303	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	1	76	theme	capillary	266:274	arg1	electrophoresis					280:294	sodium dodecyl sulfate capillary gel electrophoresis	243:294	sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE)	243:304	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	6	77	theme	monomeric	1153:1161	arg1	complexes					1191:1199	monomeric and dimeric glycerol-borate complexes	1153:1199	monomeric and dimeric glycerol-borate complexes	1153:1199	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	12	78	theme	novel	2606:2610	arg1	approach					2612:2619	This novel approach	2601:2619	This novel approach	2601:2619	This novel approach resulted in close to optimal peak shapes for the distantly migrating IgG subunits within a single run, as well as unraveled the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis.					
36095317	5	79	from	concentration	1017:1029	arg1	concentrations					1047:1060	both dextran concentrations	1034:1060	both dextran concentrations	1034:1060	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	6	80	theme	formation	1140:1148	arg1	result					1126:1131	the result	1122:1131	the result of the formation of monomeric and dimeric glycerol-borate complexes	1122:1199	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	6	80	theme	formation	1140:1148	arg1	dispersion					1093:1102	The detected electromigration dispersion	1063:1102	The detected electromigration dispersion	1063:1102	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	9	81	theme	cross-linking	2223:2235	arg1	function					2237:2244	its gel cross-linking function	2215:2244	its gel cross-linking function	2215:2244	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	8	82	theme	sieving	1776:1782	arg1	size					1796:1799	sieving matrix pore size	1776:1799	sieving matrix pore size	1776:1799	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	7	83	theme	co-ionic	1528:1535	arg1	species					1537:1543	the co-ionic species	1524:1543	the co-ionic species	1524:1543	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	8	84	from	concentrations	1684:1697	arg1	result					1724:1729	the result	1720:1729	the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1720:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	84	from	concentrations	1684:1697	arg1	shapes					1604:1609	the observed fronting and/or tailing peak shapes	1562:1609	the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations	1562:1697	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	85	theme	pore	1791:1794	arg1	size					1796:1799	sieving matrix pore size	1776:1799	sieving matrix pore size	1776:1799	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	1	86	theme	therapeutic	180:190	arg1	antibody					203:210	the therapeutic monoclonal antibody	176:210	the therapeutic monoclonal antibody omalizumab	176:221	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	10	87	dep	%	2391:2391	arg1	systems					2441:2447	boric acid-containing gel-buffer systems	2408:2447	10% dextran/640 mM boric acid-containing gel-buffer systems	2389:2447	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	7	88	theme	species	1537:1543	arg1	mobility					1512:1519	the resultant effective mobility	1488:1519	the resultant effective mobility of the co-ionic species	1488:1543	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	10	89	theme	boric	2374:2378	arg1	acid					2380:2383	dextran/340 mM boric acid	2359:2383	10% dextran/340 mM boric acid	2355:2383	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	9	90	theme	effective	2010:2018	arg1	mobility					2020:2027	the effective mobility	2006:2027	the effective mobility of the co-ionic species	2006:2051	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	1	91	theme	antibody	203:210	arg1	omalizumab					212:221	the therapeutic monoclonal antibody omalizumab	176:221	the therapeutic monoclonal antibody omalizumab	176:221	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	11	92	theme	transient	2549:2557	arg1	mobility					2559:2566	borate-gradient-mediated transient mobility	2524:2566	borate-gradient-mediated transient mobility matching in SDS-CGE of proteins	2524:2598	Based on this observation, here we introduce the concept of borate-gradient-mediated transient mobility matching in SDS-CGE of proteins.					
36095317	10	93	theme	dextran/340	2359:2369	arg1	mM					2371:2372	mM	2371:2372	mM	2371:2372	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	7	94	theme	effective	1502:1510	arg1	mobility					1512:1519	the resultant effective mobility	1488:1519	the resultant effective mobility of the co-ionic species	1488:1543	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	3	95	theme	heavy-chain	551:561	arg1	fragment					563:570	the heavy-chain fragment	547:570	the heavy-chain fragment	547:570	In case of the heavy-chain fragment, elevated borate concentrations resulted in decreasing tailing and increasing fronting with the use of higher- and lower-dextran-concentration gels, respectively.					
36095317	10	96	theme	%	2391:2391	arg1	use					2348:2350	the use	2344:2350	the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively	2344:2461	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	6	97	theme	co-ionic	1204:1211	arg1	dispersion					1093:1102	The detected electromigration dispersion	1063:1102	The detected electromigration dispersion	1063:1102	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	6	97	theme	co-ionic	1204:1211	arg1	constituents					1213:1224	co-ionic constituents	1204:1224	co-ionic constituents	1204:1224	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	9	98	theme	co-ionic	2036:2043	arg1	species					2045:2051	the co-ionic species	2032:2051	the co-ionic species	2032:2051	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	1	99	theme	subunit	159:165	arg1	peaks					167:171	the light- and heavy-chain subunit peaks	132:171	the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab	132:221	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	7	100	theme	building	1369:1376	arg1	blocks					1378:1383	the glucose building blocks	1357:1383	the glucose building blocks of the dextran polymer	1357:1406	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	8	101	with	adducts	1863:1869	arg1	ingredients					1891:1901	the gel-buffer ingredients	1876:1901	the gel-buffer ingredients	1876:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	3	102	theme	borate	582:587	arg1	concentrations					589:602	elevated borate concentrations	573:602	elevated borate concentrations	573:602	In case of the heavy-chain fragment, elevated borate concentrations resulted in decreasing tailing and increasing fronting with the use of higher- and lower-dextran-concentration gels, respectively.					
36095317	11	103	theme	mobility	2559:2566	arg1	concept					2513:2519	the concept	2509:2519	the concept of borate-gradient-mediated transient mobility matching in SDS-CGE of proteins	2509:2598	Based on this observation, here we introduce the concept of borate-gradient-mediated transient mobility matching in SDS-CGE of proteins.					
36095317	10	104	theme	acid-containing	2414:2428	arg1	systems					2441:2447	boric acid-containing gel-buffer systems	2408:2447	10% dextran/640 mM boric acid-containing gel-buffer systems	2389:2447	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	9	105	theme	analyte	2060:2066	arg1	molecules					2068:2076	the analyte molecules	2056:2076	the analyte molecules of interest	2056:2088	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	7	106	theme	anion	1346:1350	arg1	complexation					1307:1318	complexation	1307:1318	complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer	1307:1406	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	6	107	theme	electromigration	1076:1091	arg1	result					1126:1131	the result	1122:1131	the result of the formation of monomeric and dimeric glycerol-borate complexes	1122:1199	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	6	107	theme	electromigration	1076:1091	arg1	dispersion					1093:1102	The detected electromigration dispersion	1063:1102	The detected electromigration dispersion	1063:1102	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	6	107	theme	electromigration	1076:1091	arg1	constituents					1213:1224	co-ionic constituents	1204:1224	co-ionic constituents	1204:1224	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	10	108	theme	mM	2405:2406	arg1	%					2391:2391	10%	2389:2391	10% dextran/640 mM boric acid-containing gel-buffer systems	2389:2447	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	8	109	theme	fronting	1575:1582	arg1	result					1724:1729	the result	1720:1729	the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1720:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	109	theme	fronting	1575:1582	arg1	shapes					1604:1609	the observed fronting and/or tailing peak shapes	1562:1609	the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations	1562:1697	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	10	110	theme	analyte	2299:2305	arg1	fragments					2278:2286	the light- and heavy-chain fragments	2251:2286	the light- and heavy-chain fragments of the IgG analyte	2251:2305	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	12	111	theme	possible	2761:2768	arg1	solution					2770:2777	the long-sought possible solution	2745:2777	the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis	2745:2837	This novel approach resulted in close to optimal peak shapes for the distantly migrating IgG subunits within a single run, as well as unraveled the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis.					
36095317	8	112	theme	borate	1856:1861	arg1	adducts					1863:1869	the co-ionic borate adducts	1843:1869	the co-ionic borate adducts with the gel-buffer ingredients	1843:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	5	113	theme	glycerol	918:925	arg1	level					938:942	the glycerol ingredient level	914:942	the glycerol ingredient level	914:942	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	8	114	theme	tailing	1591:1597	arg1	result					1724:1729	the result	1720:1729	the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1720:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	114	theme	tailing	1591:1597	arg1	shapes					1604:1609	the observed fronting and/or tailing peak shapes	1562:1609	the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations	1562:1697	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	7	115	with	complexation	1307:1318	arg1	blocks					1378:1383	the glucose building blocks	1357:1383	the glucose building blocks of the dextran polymer	1357:1406	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	5	116	from	Increase	902:909	arg1	system					962:967	the gel-buffer system	947:967	the gel-buffer system	947:967	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	5	117	theme	level	938:942	arg1	Increase					902:909	Increase	902:909	Increase of the glycerol ingredient level in the gel-buffer system	902:967	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	0	118	from	Dispersion	17:26	arg1	Electrophoresis					68:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis	31:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins	31:94	Electromigration Dispersion in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins.					
36095317	12	119	theme	migrating	2680:2688	arg1	IgG					2690:2692	the distantly migrating IgG	2666:2692	the distantly migrating IgG	2666:2692	This novel approach resulted in close to optimal peak shapes for the distantly migrating IgG subunits within a single run, as well as unraveled the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis.					
36095317	8	120	theme	gel-buffer	1880:1889	arg1	ingredients					1891:1901	the gel-buffer ingredients	1876:1901	the gel-buffer ingredients	1876:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	11	121	theme	proteins	2591:2598	arg1	SDS-CGE					2580:2586	SDS-CGE	2580:2586	SDS-CGE of proteins	2580:2598	Based on this observation, here we introduce the concept of borate-gradient-mediated transient mobility matching in SDS-CGE of proteins.					
36095317	9	122	theme	borate	2102:2107	arg1	gels					2130:2133	borate cross-linked dextran gels	2102:2133	borate cross-linked dextran gels	2102:2133	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	8	123	from	changes	1761:1767	arg1	variation					1830:1838	the mobility variation	1817:1838	the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients	1817:1901	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	123	from	changes	1761:1767	arg1	viscosity					1802:1810	viscosity	1802:1810	viscosity	1802:1810	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	123	from	changes	1761:1767	arg1	size					1796:1799	sieving matrix pore size	1776:1799	sieving matrix pore size	1776:1799	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	8	123	from	changes	1761:1767	arg1	pH					1772:1773	pH	1772:1773	pH	1772:1773	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	10	124	theme	IgG	2295:2297	arg1	analyte					2299:2305	the IgG analyte	2291:2305	the IgG analyte	2291:2305	For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively.					
36095317	5	125	theme	borate	1010:1015	arg1	effect					985:990	the same effect	976:990	the same effect	976:990	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	5	125	theme	borate	1010:1015	arg1	concentration					1017:1029	the increasing borate concentration	995:1029	the increasing borate concentration in both dextran concentrations	995:1060	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	8	126	theme	antibody	1629:1636	arg1	fragments					1638:1646	the monoclonal antibody fragments	1614:1646	the monoclonal antibody fragments in SDS-CGE	1614:1657	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	9	127	theme	dextran	2122:2128	arg1	gels					2130:2133	borate cross-linked dextran gels	2102:2133	borate cross-linked dextran gels	2102:2133	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	4	128	theme	increased	790:798	arg1	fronting					800:807	increased fronting	790:807	increased fronting	790:807	The light-chain fragment, on the other hand, exhibited increased fronting with increasing borate concentration for both dextran concentrations examined in this study.					
36095317	8	129	from	shapes	1604:1609	arg1	SDS-CGE					1651:1657	SDS-CGE	1651:1657	SDS-CGE	1651:1657	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	7	130	theme	polymer	1400:1406	arg1	blocks					1378:1383	the glucose building blocks	1357:1383	the glucose building blocks of the dextran polymer	1357:1406	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	2	131	theme	electromigration	408:423	arg1	shifts					436:441	electromigration dispersion shifts	408:441	electromigration dispersion shifts for both low (2%)- and high (10%)-dextran-concentration gels	408:502	Increasing boric acid content (340-640 mM) caused electromigration dispersion shifts for both low (2%)- and high (10%)-dextran-concentration gels in all gel-buffer compositions.					
36095317	0	132	theme	Sodium	31:36	arg1	Electrophoresis					68:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis	31:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins	31:94	Electromigration Dispersion in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins.					
36095317	1	133	theme	borate	312:317	arg1	matrices					348:355	borate cross-linked dextran sieving matrices	312:355	borate cross-linked dextran sieving matrices	312:355	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	4	134	theme	other	768:772	arg1	hand					774:777	the other hand	764:777	the other hand	764:777	The light-chain fragment, on the other hand, exhibited increased fronting with increasing borate concentration for both dextran concentrations examined in this study.					
36095317	0	135	theme	Sulfate	46:52	arg1	Electrophoresis					68:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis	31:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins	31:94	Electromigration Dispersion in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins.					
36095317	1	136	theme	dextran	332:338	arg1	matrices					348:355	borate cross-linked dextran sieving matrices	312:355	borate cross-linked dextran sieving matrices	312:355	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	7	137	from	decrease	1476:1483	arg1	mobility					1512:1519	the resultant effective mobility	1488:1519	the resultant effective mobility of the co-ionic species	1488:1543	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	9	138	theme	dispersion-mediated	1927:1945	arg1	broadening					1952:1961	electromigration dispersion-mediated band broadening	1910:1961	electromigration dispersion-mediated band broadening	1910:1961	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	8	139	theme	boric	1673:1677	arg1	concentrations					1684:1697	increasing boric acid concentrations	1662:1697	increasing boric acid concentrations	1662:1697	We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients.					
36095317	9	140	theme	boric	2156:2160	arg1	acid					2162:2165	the boric acid	2152:2165	the boric acid concentration	2152:2179	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	0	141	theme	Gel	64:66	arg1	Electrophoresis					68:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis	31:82	Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins	31:94	Electromigration Dispersion in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins.					
36095317	7	142	theme	glucose	1361:1367	arg1	blocks					1378:1383	the glucose building blocks	1357:1383	the glucose building blocks of the dextran polymer	1357:1406	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	9	143	theme	concentration	2167:2179	arg1	optimization					2136:2147	optimization	2136:2147	optimization of the boric acid concentration	2136:2179	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	4	144	theme	dextran	855:861	arg1	concentrations					863:876	both dextran concentrations	850:876	both dextran concentrations examined in this study	850:899	The light-chain fragment, on the other hand, exhibited increased fronting with increasing borate concentration for both dextran concentrations examined in this study.					
36095317	6	145	theme	glycerol-borate	1175:1189	arg1	complexes					1191:1199	monomeric and dimeric glycerol-borate complexes	1153:1199	monomeric and dimeric glycerol-borate complexes	1153:1199	The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate.					
36095317	9	146	theme	special	2190:2196	arg1	consideration					2198:2210	special consideration	2190:2210	special consideration of its gel cross-linking function	2190:2244	While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function.					
36095317	1	147	link	cross-linked	319:330	arg1	matrices					348:355	borate cross-linked dextran sieving matrices	312:355	borate cross-linked dextran sieving matrices	312:355	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	2	148	theme	boric	369:373	arg1	content					380:386	boric acid content	369:386	boric acid content (340-640 mM)	369:399	Increasing boric acid content (340-640 mM) caused electromigration dispersion shifts for both low (2%)- and high (10%)-dextran-concentration gels in all gel-buffer compositions.					
36095317	2	148	theme	boric	369:373	arg1	mM					397:398	340-640 mM	389:398	340-640 mM	389:398	Increasing boric acid content (340-640 mM) caused electromigration dispersion shifts for both low (2%)- and high (10%)-dextran-concentration gels in all gel-buffer compositions.					
36095317	1	149	theme	sulfate	258:264	arg1	SDS-CGE					297:303	SDS-CGE	297:303	SDS-CGE	297:303	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	1	149	theme	sulfate	258:264	arg1	electrophoresis					280:294	sodium dodecyl sulfate capillary gel electrophoresis	243:294	sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE)	243:304	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	5	150	theme	dextran	1039:1045	arg1	concentrations					1047:1060	both dextran concentrations	1034:1060	both dextran concentrations	1034:1060	Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations.					
36095317	7	151	theme	tetrahydroxyborate	1327:1344	arg1	anion					1346:1350	the tetrahydroxyborate anion	1323:1350	the tetrahydroxyborate anion	1323:1350	In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species.					
36095317	4	152	theme	borate	825:830	arg1	concentration					832:844	borate concentration	825:844	borate concentration	825:844	The light-chain fragment, on the other hand, exhibited increased fronting with increasing borate concentration for both dextran concentrations examined in this study.					
36095317	1	153	theme	gel	276:278	arg1	SDS-CGE					297:303	SDS-CGE	297:303	SDS-CGE	297:303	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36095317	1	153	theme	gel	276:278	arg1	electrophoresis					280:294	sodium dodecyl sulfate capillary gel electrophoresis	243:294	sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE)	243:304	The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices.					
36604524	1	0	theme	thylakoid	156:164	arg1	membranes					166:174	thylakoid membranes	156:174	thylakoid membranes	156:174	The lipid composition of thylakoid membranes is conserved from cyanobacteria to green plants.					
36604524	0	1	theme	galactolipid	74:85	arg1	levels					87:92	increased galactolipid levels	64:92	increased galactolipid levels in Synechococcus elongatus PCC 7942	64:128	Changes in intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942.					
36604524	7	2	theme	energy	1220:1225	arg1	storage					1227:1233	energy storage	1220:1233	energy storage	1220:1233	Furthermore, physiological analyses indicated that the bioenergetic state of SeGPT is altered toward energy storage, as indicated by increased levels of intracellular ATP and glycogen.					
36604524	1	3	theme	membranes	166:174	arg1	composition					141:151	The lipid composition	131:151	The lipid composition of thylakoid membranes	131:174	The lipid composition of thylakoid membranes is conserved from cyanobacteria to green plants.					
36604524	6	4	theme	membrane	1075:1082	arg1	development					1084:1094	thylakoid membrane development	1065:1094	thylakoid membrane development	1065:1094	Microscopic observations revealed that cell division and thylakoid membrane development are impaired in SeGPT.					
36604524	0	5	theme	Synechococcus	97:109	arg1	7942					125:128	Synechococcus elongatus PCC 7942	97:128	Synechococcus elongatus PCC 7942	97:128	Changes in intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942.					
36604524	0	6	from	Changes	0:6	arg1	states					50:55	intracellular energetic and metabolite states	11:55	intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942	11:128	Changes in intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942.					
36604524	4	7	theme	S.	832:833	arg1	7942					849:852	wild-type S. elongatus PCC 7942	822:852	wild-type S. elongatus PCC 7942	822:852	SeGPT exhibited increased galactolipid content and could grow photoautotrophically, but its growth rate was slower than that of wild-type S. elongatus PCC 7942.					
36604524	6	8	theme	thylakoid	1065:1073	arg1	development					1084:1094	thylakoid membrane development	1065:1094	thylakoid membrane development	1065:1094	Microscopic observations revealed that cell division and thylakoid membrane development are impaired in SeGPT.					
36604524	7	9	theme	glycogen	1294:1301	arg1	levels					1262:1267	increased levels	1252:1267	increased levels of intracellular ATP and glycogen	1252:1301	Furthermore, physiological analyses indicated that the bioenergetic state of SeGPT is altered toward energy storage, as indicated by increased levels of intracellular ATP and glycogen.					
36604524	8	10	theme	synthesis	1432:1440	arg1	system					1442:1447	the lipid synthesis system	1422:1447	the lipid synthesis system	1422:1447	We hereby report that we have identified a new promising candidate as a platform for material production by modifying the lipid synthesis system in this way.					
36604524	4	11	theme	wild-type	822:830	arg1	7942					849:852	wild-type S. elongatus PCC 7942	822:852	wild-type S. elongatus PCC 7942	822:852	SeGPT exhibited increased galactolipid content and could grow photoautotrophically, but its growth rate was slower than that of wild-type S. elongatus PCC 7942.					
36604524	5	12	theme	cell	988:991	arg1	proliferation					993:1005	cell proliferation	988:1005	cell proliferation	988:1005	In the present study, we investigated pleiotropic effects that occur in SeGPT and determined how its increased lipid content affects cell proliferation.					
36604524	0	13	theme	PCC	121:123	arg1	7942					125:128	Synechococcus elongatus PCC 7942	97:128	Synechococcus elongatus PCC 7942	97:128	Changes in intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942.					
36604524	5	14	theme	lipid	966:970	arg1	content					972:978	its increased lipid content	952:978	its increased lipid content	952:978	In the present study, we investigated pleiotropic effects that occur in SeGPT and determined how its increased lipid content affects cell proliferation.					
36604524	5	15	theme	pleiotropic	893:903	arg1	effects					905:911	pleiotropic effects	893:911	pleiotropic effects that occur in SeGPT	893:931	In the present study, we investigated pleiotropic effects that occur in SeGPT and determined how its increased lipid content affects cell proliferation.					
36604524	3	16	theme	PCC	507:509	arg1	7942					511:514	the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942	441:514	the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942	441:514	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	0	17	dep	Synechococcus	97:109	arg1	elongatus					111:119	elongatus	111:119	elongatus	111:119	Changes in intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942.					
36604524	3	18	theme	unicellular	445:455	arg1	7942					511:514	the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942	441:514	the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942	441:514	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	7	19	theme	ATP	1286:1288	arg1	levels					1262:1267	increased levels	1252:1267	increased levels of intracellular ATP and glycogen	1252:1301	Furthermore, physiological analyses indicated that the bioenergetic state of SeGPT is altered toward energy storage, as indicated by increased levels of intracellular ATP and glycogen.					
36604524	5	20	theme	increased	956:964	arg1	content					972:978	its increased lipid content	952:978	its increased lipid content	952:978	In the present study, we investigated pleiotropic effects that occur in SeGPT and determined how its increased lipid content affects cell proliferation.					
36604524	7	21	theme	SeGPT	1196:1200	arg1	state					1187:1191	the bioenergetic state	1170:1191	the bioenergetic state of SeGPT	1170:1200	Furthermore, physiological analyses indicated that the bioenergetic state of SeGPT is altered toward energy storage, as indicated by increased levels of intracellular ATP and glycogen.					
36604524	4	22	theme	elongatus	835:843	arg1	7942					849:852	wild-type S. elongatus PCC 7942	822:852	wild-type S. elongatus PCC 7942	822:852	SeGPT exhibited increased galactolipid content and could grow photoautotrophically, but its growth rate was slower than that of wild-type S. elongatus PCC 7942.					
36604524	3	23	theme	rod-shaped	457:466	arg1	7942					511:514	the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942	441:514	the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942	441:514	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	5	24	theme	present	862:868	arg1	study					870:874	the present study	858:874	the present study	858:874	In the present study, we investigated pleiotropic effects that occur in SeGPT and determined how its increased lipid content affects cell proliferation.					
36604524	7	25	theme	bioenergetic	1174:1185	arg1	state					1187:1191	the bioenergetic state	1170:1191	the bioenergetic state of SeGPT	1170:1200	Furthermore, physiological analyses indicated that the bioenergetic state of SeGPT is altered toward energy storage, as indicated by increased levels of intracellular ATP and glycogen.					
36604524	3	26	theme	synthesis	544:552	arg1	pathways					554:561	the synthesis pathways	540:561	the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol	540:642	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	0	27	theme	energetic	25:33	arg1	states					50:55	intracellular energetic and metabolite states	11:55	intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942	11:128	Changes in intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942.					
36604524	6	28	theme	cell	1047:1050	arg1	division					1052:1059	cell division	1047:1059	cell division	1047:1059	Microscopic observations revealed that cell division and thylakoid membrane development are impaired in SeGPT.					
36604524	2	29	theme	membranes	312:320	arg1	components					288:297	the major components	278:297	the major components of thylakoid membranes	278:320	However, the biosynthetic pathways of galactolipids, the major components of thylakoid membranes, are known to differ substantially between cyanobacteria and green plants.					
36604524	2	29	theme	membranes	312:320	arg1	galactolipids					263:275	galactolipids	263:275	galactolipids	263:275	However, the biosynthetic pathways of galactolipids, the major components of thylakoid membranes, are known to differ substantially between cyanobacteria and green plants.					
36604524	3	30	theme	elongatus	497:505	arg1	7942					511:514	the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942	441:514	the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942	441:514	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	0	31	theme	intracellular	11:23	arg1	states					50:55	intracellular energetic and metabolite states	11:55	intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942	11:128	Changes in intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942.					
36604524	2	32	theme	major	282:286	arg1	components					288:297	the major components	278:297	the major components of thylakoid membranes	278:320	However, the biosynthetic pathways of galactolipids, the major components of thylakoid membranes, are known to differ substantially between cyanobacteria and green plants.					
36604524	2	32	theme	major	282:286	arg1	galactolipids					263:275	galactolipids	263:275	galactolipids	263:275	However, the biosynthetic pathways of galactolipids, the major components of thylakoid membranes, are known to differ substantially between cyanobacteria and green plants.					
36604524	0	33	from	levels	87:92	arg1	7942					125:128	Synechococcus elongatus PCC 7942	97:128	Synechococcus elongatus PCC 7942	97:128	Changes in intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942.					
36604524	2	34	theme	thylakoid	302:310	arg1	membranes					312:320	thylakoid membranes	302:320	thylakoid membranes	302:320	However, the biosynthetic pathways of galactolipids, the major components of thylakoid membranes, are known to differ substantially between cyanobacteria and green plants.					
36604524	1	35	theme	green	211:215	arg1	plants					217:222	green plants	211:222	green plants	211:222	The lipid composition of thylakoid membranes is conserved from cyanobacteria to green plants.					
36604524	0	36	theme	metabolite	39:48	arg1	states					50:55	intracellular energetic and metabolite states	11:55	intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942	11:128	Changes in intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942.					
36604524	4	37	theme	increased	710:718	arg1	content					733:739	increased galactolipid content	710:739	increased galactolipid content	710:739	SeGPT exhibited increased galactolipid content and could grow photoautotrophically, but its growth rate was slower than that of wild-type S. elongatus PCC 7942.					
36604524	2	38	theme	biosynthetic	238:249	arg1	pathways					251:258	the biosynthetic pathways	234:258	the biosynthetic pathways of galactolipids, the major components of thylakoid membranes,	234:321	However, the biosynthetic pathways of galactolipids, the major components of thylakoid membranes, are known to differ substantially between cyanobacteria and green plants.					
36604524	3	39	theme	cyanobacterium	468:481	arg1	7942					511:514	the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942	441:514	the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942	441:514	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	8	40	theme	promising	1351:1359	arg1	platform					1376:1383	a platform	1374:1383	a platform for material production by modifying the lipid synthesis system in this way	1374:1459	We hereby report that we have identified a new promising candidate as a platform for material production by modifying the lipid synthesis system in this way.					
36604524	8	40	theme	promising	1351:1359	arg1	candidate					1361:1369	a new promising candidate	1345:1369	a new promising candidate	1345:1369	We hereby report that we have identified a new promising candidate as a platform for material production by modifying the lipid synthesis system in this way.					
36604524	0	41	theme	due	57:59	arg1	states					50:55	intracellular energetic and metabolite states	11:55	intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942	11:128	Changes in intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942.					
36604524	6	42	theme	Microscopic	1008:1018	arg1	observations					1020:1031	Microscopic observations	1008:1031	Microscopic observations	1008:1031	Microscopic observations revealed that cell division and thylakoid membrane development are impaired in SeGPT.					
36604524	8	43	theme	lipid	1426:1430	arg1	system					1442:1447	the lipid synthesis system	1422:1447	the lipid synthesis system	1422:1447	We hereby report that we have identified a new promising candidate as a platform for material production by modifying the lipid synthesis system in this way.					
36604524	4	44	theme	growth	786:791	arg1	rate					793:796	its growth rate	782:796	its growth rate	782:796	SeGPT exhibited increased galactolipid content and could grow photoautotrophically, but its growth rate was slower than that of wild-type S. elongatus PCC 7942.					
36604524	7	45	theme	physiological	1132:1144	arg1	analyses					1146:1153	physiological analyses	1132:1153	physiological analyses	1132:1153	Furthermore, physiological analyses indicated that the bioenergetic state of SeGPT is altered toward energy storage, as indicated by increased levels of intracellular ATP and glycogen.					
36604524	3	46	dep	cyanobacterium	468:481	arg1	Synechococcus					483:495	Synechococcus	483:495	Synechococcus	483:495	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	3	47	theme	green	680:684	arg1	plants					686:691	green plants	680:691	green plants	680:691	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	3	48	theme	7942	511:514	arg1	SeGPT					524:528	SeGPT	524:528	SeGPT	524:528	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	3	48	theme	7942	511:514	arg1	transformant					425:436	a transformant	423:436	a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942	423:514	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	3	49	theme	galactolipids	570:582	arg1	pathways					554:561	the synthesis pathways	540:561	the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol	540:642	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	4	50	theme	PCC	845:847	arg1	7942					849:852	wild-type S. elongatus PCC 7942	822:852	wild-type S. elongatus PCC 7942	822:852	SeGPT exhibited increased galactolipid content and could grow photoautotrophically, but its growth rate was slower than that of wild-type S. elongatus PCC 7942.					
36604524	2	51	theme	galactolipids	263:275	arg1	pathways					251:258	the biosynthetic pathways	234:258	the biosynthetic pathways of galactolipids, the major components of thylakoid membranes,	234:321	However, the biosynthetic pathways of galactolipids, the major components of thylakoid membranes, are known to differ substantially between cyanobacteria and green plants.					
36604524	1	52	theme	lipid	135:139	arg1	composition					141:151	The lipid composition	131:151	The lipid composition of thylakoid membranes	131:174	The lipid composition of thylakoid membranes is conserved from cyanobacteria to green plants.					
36604524	0	53	theme	increased	64:72	arg1	levels					87:92	increased galactolipid levels	64:92	increased galactolipid levels in Synechococcus elongatus PCC 7942	64:128	Changes in intracellular energetic and metabolite states due to increased galactolipid levels in Synechococcus elongatus PCC 7942.					
36604524	3	54	dep	galactolipids	570:582	arg1	monogalactosyldiacylglycerol					584:611	monogalactosyldiacylglycerol	584:611	monogalactosyldiacylglycerol	584:611	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	3	54	dep	galactolipids	570:582	arg1	galactolipids					570:582	the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol	566:642	the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol	566:642	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	3	54	dep	galactolipids	570:582	arg1	digalactosyldiacylglycerol					617:642	digalactosyldiacylglycerol	617:642	digalactosyldiacylglycerol	617:642	We previously reported on a transformant of the unicellular rod-shaped cyanobacterium Synechococcus elongatus PCC 7942, namely SeGPT, in which the synthesis pathways of the galactolipids monogalactosyldiacylglycerol and digalactosyldiacylglycerol are completely replaced by those of green plants.					
36604524	2	55	theme	green	383:387	arg1	plants					389:394	green plants	383:394	green plants	383:394	However, the biosynthetic pathways of galactolipids, the major components of thylakoid membranes, are known to differ substantially between cyanobacteria and green plants.					
36604524	4	56	theme	galactolipid	720:731	arg1	content					733:739	increased galactolipid content	710:739	increased galactolipid content	710:739	SeGPT exhibited increased galactolipid content and could grow photoautotrophically, but its growth rate was slower than that of wild-type S. elongatus PCC 7942.					
36604524	7	57	theme	intracellular	1272:1284	arg1	ATP					1286:1288	intracellular ATP	1272:1288	intracellular ATP	1272:1288	Furthermore, physiological analyses indicated that the bioenergetic state of SeGPT is altered toward energy storage, as indicated by increased levels of intracellular ATP and glycogen.					
36604524	7	58	theme	increased	1252:1260	arg1	levels					1262:1267	increased levels	1252:1267	increased levels of intracellular ATP and glycogen	1252:1301	Furthermore, physiological analyses indicated that the bioenergetic state of SeGPT is altered toward energy storage, as indicated by increased levels of intracellular ATP and glycogen.					
36604524	8	59	theme	new	1347:1349	arg1	platform					1376:1383	a platform	1374:1383	a platform for material production by modifying the lipid synthesis system in this way	1374:1459	We hereby report that we have identified a new promising candidate as a platform for material production by modifying the lipid synthesis system in this way.					
36604524	8	59	theme	new	1347:1349	arg1	candidate					1361:1369	a new promising candidate	1345:1369	a new promising candidate	1345:1369	We hereby report that we have identified a new promising candidate as a platform for material production by modifying the lipid synthesis system in this way.					
36604524	8	60	theme	material	1389:1396	arg1	production					1398:1407	material production	1389:1407	material production by modifying the lipid synthesis system in this way	1389:1459	We hereby report that we have identified a new promising candidate as a platform for material production by modifying the lipid synthesis system in this way.					
37249588	8	0	theme	Carnobacterium	1117:1130	arg1	maltaromaticum					1132:1145	Carnobacterium maltaromaticum	1117:1145	Carnobacterium maltaromaticum	1117:1145	Carnobacterium maltaromaticum, unknown Enterobacteriaceae, and Yersinia frederiksenii have genes for chitin and cellulose degradation, acetate, and glutamate production.					
37249588	6	1	theme	fatty	859:863	arg1	degradation					870:880	fatty acid degradation	859:880	fatty acid degradation	859:880	Meanwhile, fatty acid degradation and betalain biosynthesis pathways are enriched in late overwintering period.					
37249588	7	2	dep	bins	1018:1021	arg1	%					1040:1040	completeness >70%	1024:1040	completeness >70%	1024:1040	Furthermore, metagenomic binning obtained 91 high-quality bins (completeness >70% and contamination <10%), 5 of which enriched in late overwintering period.					
37249588	7	2	dep	bins	1018:1021	arg1	contamination					1046:1058	contamination	1046:1058	contamination	1046:1058	Furthermore, metagenomic binning obtained 91 high-quality bins (completeness >70% and contamination <10%), 5 of which enriched in late overwintering period.					
37249588	13	3	theme	Gut	2178:2180	arg1	microbiota					2182:2191	Gut microbiota	2178:2191	Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota	2178:2278	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	13	4	from	changes	2139:2145	arg1	abundance					2166:2174	core microbiota abundance	2150:2174	core microbiota abundance	2150:2174	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	1	5	theme	environmental	163:175	arg1	adaptation					177:186	environmental adaptation	163:186	environmental adaptation in migratory birds	163:205	Less is known about the role of gut microbiota in overwintering environmental adaptation in migratory birds.					
37249588	8	6	theme	cellulose	1229:1237	arg1	degradation					1239:1249	cellulose degradation	1229:1249	cellulose degradation	1229:1249	Carnobacterium maltaromaticum, unknown Enterobacteriaceae, and Yersinia frederiksenii have genes for chitin and cellulose degradation, acetate, and glutamate production.					
37249588	12	7	theme	late	1991:1994	arg1	period					2010:2015	late overwintering period	1991:2015	late overwintering period	1991:2015	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	6	8	theme	late	933:936	arg1	period					952:957	late overwintering period	933:957	late overwintering period	933:957	Meanwhile, fatty acid degradation and betalain biosynthesis pathways are enriched in late overwintering period.					
37249588	13	9	from	Differences	2032:2042	arg1	function					2062:2069	gut microbiota function	2047:2069	gut microbiota function during overwintering period of black-necked cranes	2047:2120	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	12	10	theme	resistance	1964:1973	arg1	genes					1975:1979	antibiotic resistance genes	1953:1979	antibiotic resistance genes (ARGs)	1953:1986	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	12	10	theme	resistance	1964:1973	arg1	Proteobacteria					1878:1891	enriched Proteobacteria	1869:1891	enriched Proteobacteria	1869:1891	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	12	10	theme	resistance	1964:1973	arg1	ARGs					1982:1985	ARGs	1982:1985	ARGs	1982:1985	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	5	11	theme	chitin	817:822	arg1	degradation					802:812	degradation	802:812	degradation of chitin, cellulose, and lipids	802:845	Based on previous reports, these species are associated with degradation of chitin, cellulose, and lipids.					
37249588	8	12	theme	glutamate	1265:1273	arg1	production					1275:1284	glutamate production	1265:1284	glutamate production	1265:1284	Carnobacterium maltaromaticum, unknown Enterobacteriaceae, and Yersinia frederiksenii have genes for chitin and cellulose degradation, acetate, and glutamate production.					
37249588	7	13	theme	completeness	1024:1035	arg1	%					1040:1040	completeness >70%	1024:1040	completeness >70%	1024:1040	Furthermore, metagenomic binning obtained 91 high-quality bins (completeness >70% and contamination <10%), 5 of which enriched in late overwintering period.					
37249588	13	14	theme	black-necked	2102:2113	arg1	cranes					2115:2120	black-necked cranes	2102:2120	black-necked cranes	2102:2120	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	3	15	theme	significant	522:532	arg1	change					534:539	no significant change	519:539	no significant change in microbial diversity among overwintering periods	519:590	The results demonstrated no significant change in microbial diversity among overwintering periods.					
37249588	7	16	theme	metagenomic	973:983	arg1	binning					985:991	metagenomic binning	973:991	metagenomic binning	973:991	Furthermore, metagenomic binning obtained 91 high-quality bins (completeness >70% and contamination <10%), 5 of which enriched in late overwintering period.					
37249588	10	17	contain	has	1482:1484	arg1	maltaromaticum					1467:1480	C. maltaromaticum	1464:1480	C. maltaromaticum	1464:1480	C. maltaromaticum has genes for pyridoxal synthesis.					
37249588	10	17	contain	has	1482:1484	arg2	genes					1486:1490	genes	1486:1490	genes for pyridoxal synthesis	1486:1514	C. maltaromaticum has genes for pyridoxal synthesis.					
37249588	6	18	theme	betalain	886:893	arg1	pathways					908:915	betalain biosynthesis pathways	886:915	betalain biosynthesis pathways	886:915	Meanwhile, fatty acid degradation and betalain biosynthesis pathways are enriched in late overwintering period.					
37249588	4	19	theme	Proteobacteria	679:692	arg1	species					694:700	15 Proteobacteria species	676:700	15 Proteobacteria species enriched in late overwintering period	676:738	Analysis of compositions of microbiomes with bias correction (ANCOM-BC) determined 15 Proteobacteria species enriched in late overwintering period.					
37249588	4	20	theme	late	714:717	arg1	period					733:738	late overwintering period	714:738	late overwintering period	714:738	Analysis of compositions of microbiomes with bias correction (ANCOM-BC) determined 15 Proteobacteria species enriched in late overwintering period.					
37249588	11	21	theme	vitamin	1747:1753	arg1	pyridoxal					1755:1763	vitamin pyridoxal	1747:1763	vitamin pyridoxal	1747:1763	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	7	22	theme	bins	1018:1021	arg1	bins					1018:1021	91 high-quality bins	1002:1021	91 high-quality bins (completeness >70% and contamination <10%)	1002:1064	Furthermore, metagenomic binning obtained 91 high-quality bins (completeness >70% and contamination <10%), 5 of which enriched in late overwintering period.					
37249588	7	22	theme	bins	1018:1021	arg1	5					1067:1067	5	1067:1067	5	1067:1067	Furthermore, metagenomic binning obtained 91 high-quality bins (completeness >70% and contamination <10%), 5 of which enriched in late overwintering period.					
37249588	3	23	theme	overwintering	570:582	arg1	periods					584:590	overwintering periods	570:590	overwintering periods	570:590	The results demonstrated no significant change in microbial diversity among overwintering periods.					
37249588	1	24	from	adaptation	177:186	arg1	birds					201:205	migratory birds	191:205	migratory birds	191:205	Less is known about the role of gut microbiota in overwintering environmental adaptation in migratory birds.					
37249588	2	25	theme	functional	425:434	arg1	characteristics					436:450	microbial taxonomic and functional characteristics	401:450	microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis)	401:491	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	1	26	theme	gut	131:133	arg1	microbiota					135:144	gut microbiota	131:144	gut microbiota	131:144	Less is known about the role of gut microbiota in overwintering environmental adaptation in migratory birds.					
37249588	2	27	theme	taxonomic	411:419	arg1	characteristics					436:450	microbial taxonomic and functional characteristics	401:450	microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis)	401:491	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	2	28	theme	crane	468:472	arg1	characteristics					436:450	microbial taxonomic and functional characteristics	401:450	microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis)	401:491	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	2	29	theme	fresh	253:257	arg1	n = 24					274:279	n = 24	274:279	n = 24	274:279	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	2	29	theme	fresh	253:257	arg1	samples					265:271	fresh fecal samples	253:271	fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late)	253:379	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	9	30	contain	has	1422:1424	arg1	latter					1415:1420	latter	1415:1420	latter	1415:1420	Unknown Enterobacteriaceae and Y. frederiksenii hold genes for synthesis of 10 essential amino acids required by birds, and the latter has genes for γ-aminobutyrate production.					
37249588	9	30	contain	has	1422:1424	arg2	genes					1426:1430	genes	1426:1430	genes for γ-aminobutyrate production	1426:1461	Unknown Enterobacteriaceae and Y. frederiksenii hold genes for synthesis of 10 essential amino acids required by birds, and the latter has genes for γ-aminobutyrate production.					
37249588	9	31	theme	Unknown	1287:1293	arg1	Enterobacteriaceae					1295:1312	Unknown Enterobacteriaceae	1287:1312	Unknown Enterobacteriaceae	1287:1312	Unknown Enterobacteriaceae and Y. frederiksenii hold genes for synthesis of 10 essential amino acids required by birds, and the latter has genes for γ-aminobutyrate production.					
37249588	2	32	dep	characterize	384:395	arg1	gut					397:399	gut	397:399	gut	397:399	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	10	33	theme	C.	1464:1465	arg1	maltaromaticum					1467:1480	C. maltaromaticum	1464:1480	C. maltaromaticum	1464:1480	C. maltaromaticum has genes for pyridoxal synthesis.					
37249588	13	34	theme	microbiota	2051:2060	arg1	function					2062:2069	gut microbiota function	2047:2069	gut microbiota function during overwintering period of black-necked cranes	2047:2120	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	13	35	dep	POINTS	2022:2027	arg1	depend					2122:2127	depend	2122:2127	depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period	2122:2368	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	2	36	dep	overwintering	312:324	arg1	Dec					327:329	Dec	327:329	Dec	327:329	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	0	37	theme	Gut	0:2	arg1	microbiota					4:13	Gut microbiota	0:13	Gut microbiota	0:13	Gut microbiota enhance energy accumulation of black-necked crane to cope with impending migration.					
37249588	12	38	theme	carbohydrate-active	1911:1929	arg1	CAZymes					1940:1946	CAZymes	1940:1946	CAZymes	1940:1946	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	12	38	theme	carbohydrate-active	1911:1929	arg1	enzymes					1931:1937	more carbohydrate-active enzymes	1906:1937	more carbohydrate-active enzymes (CAZymes)	1906:1947	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	12	38	theme	carbohydrate-active	1911:1929	arg1	Proteobacteria					1878:1891	enriched Proteobacteria	1869:1891	enriched Proteobacteria	1869:1891	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	2	39	dep	Dec	327:329	arg1	Mar					370:372	Mar	370:372	Mar	370:372	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	2	39	dep	Dec	327:329	arg1	early					332:336	early	332:336	early	332:336	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	2	39	dep	Dec	327:329	arg1	late					375:378	late	375:378	late	375:378	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	2	39	dep	Dec	327:329	arg1	Feb					354:356	Feb	354:356	Feb	354:356	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	2	39	dep	Dec	327:329	arg1	Jan					339:341	Jan	339:341	Jan	339:341	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	2	39	dep	Dec	327:329	arg1	middle					359:364	middle II	359:367	Dec: early; Jan: middle I; Feb: middle II; Mar	327:372	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	2	39	dep	Dec	327:329	arg1	middle					344:349	middle I	344:351	Dec: early; Jan: middle I; Feb: middle II; Mar	327:372	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	13	40	theme	Gut	2265:2267	arg1	microbiota					2269:2278	Gut microbiota	2265:2278	Gut microbiota	2265:2278	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	4	41	with	microbiomes	621:631	arg1	ANCOM-BC					655:662	ANCOM-BC	655:662	ANCOM-BC	655:662	Analysis of compositions of microbiomes with bias correction (ANCOM-BC) determined 15 Proteobacteria species enriched in late overwintering period.					
37249588	4	41	with	microbiomes	621:631	arg1	correction					643:652	bias correction	638:652	bias correction (ANCOM-BC)	638:663	Analysis of compositions of microbiomes with bias correction (ANCOM-BC) determined 15 Proteobacteria species enriched in late overwintering period.					
37249588	5	42	theme	previous	750:757	arg1	reports					759:765	previous reports	750:765	previous reports	750:765	Based on previous reports, these species are associated with degradation of chitin, cellulose, and lipids.					
37249588	7	43	theme	late	1090:1093	arg1	period					1109:1114	late overwintering period	1090:1114	late overwintering period	1090:1114	Furthermore, metagenomic binning obtained 91 high-quality bins (completeness >70% and contamination <10%), 5 of which enriched in late overwintering period.					
37249588	11	44	theme	amino	1731:1735	arg1	acids					1737:1741	essential amino acids	1721:1741	essential amino acids	1721:1741	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	11	45	theme	host	1576:1579	arg1	diet					1581:1584	the host diet	1572:1584	the host diet	1572:1584	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	11	46	theme	host	1841:1844	arg1	feeding					1846:1852	host feeding	1841:1852	host feeding	1841:1852	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	9	47	theme	amino	1376:1380	arg1	acids					1382:1386	10 essential amino acids	1363:1386	10 essential amino acids required by birds	1363:1404	Unknown Enterobacteriaceae and Y. frederiksenii hold genes for synthesis of 10 essential amino acids required by birds, and the latter has genes for γ-aminobutyrate production.					
37249588	5	48	theme	cellulose	825:833	arg1	degradation					802:812	degradation	802:812	degradation of chitin, cellulose, and lipids	802:845	Based on previous reports, these species are associated with degradation of chitin, cellulose, and lipids.					
37249588	13	49	theme	black-necked	2196:2207	arg1	crane					2209:2213	black-necked crane	2196:2213	black-necked crane adapted to the diet during overwintering period • Gut microbiota	2196:2278	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	11	50	dep	microbiota	1547:1556	arg1	help					1594:1597	help	1594:1597	may help black-necked cranes in pre-migratory energy accumulation	1590:1654	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	11	50	dep	microbiota	1547:1556	arg1	adapted					1561:1567	adapted	1561:1567	is adapted to the host diet	1558:1584	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	13	51	theme	KEY	2018:2020	arg1	POINTS					2022:2027	KEY POINTS	2018:2027	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.	2018:2369	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	13	52	theme	microbiota	2155:2164	arg1	abundance					2166:2174	core microbiota abundance	2150:2174	core microbiota abundance	2150:2174	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	6	53	theme	acid	865:868	arg1	degradation					870:880	fatty acid degradation	859:880	fatty acid degradation	859:880	Meanwhile, fatty acid degradation and betalain biosynthesis pathways are enriched in late overwintering period.					
37249588	11	54	theme	pre-migratory	1622:1634	arg1	accumulation					1643:1654	pre-migratory energy accumulation	1622:1654	pre-migratory energy accumulation	1622:1654	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	11	55	theme	gut	1543:1545	arg1	microbiota					1547:1556	the gut microbiota	1539:1556	the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation	1539:1654	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	12	56	theme	overwintering	1996:2008	arg1	period					2010:2015	late overwintering period	1991:2015	late overwintering period	1991:2015	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	11	57	theme	complex	1673:1679	arg1	polysaccharide					1681:1694	the complex polysaccharide	1669:1694	the complex polysaccharide in their diet	1669:1708	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	13	58	theme	late	2344:2347	arg1	period					2363:2368	the late overwintering period	2340:2368	the late overwintering period	2340:2368	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	0	59	theme	black-necked	46:57	arg1	crane					59:63	black-necked crane	46:63	black-necked crane	46:63	Gut microbiota enhance energy accumulation of black-necked crane to cope with impending migration.					
37249588	4	60	theme	compositions	605:616	arg1	Analysis					593:600	Analysis	593:600	Analysis of compositions of microbiomes with bias correction (ANCOM-BC)	593:663	Analysis of compositions of microbiomes with bias correction (ANCOM-BC) determined 15 Proteobacteria species enriched in late overwintering period.					
37249588	8	61	theme	unknown	1148:1154	arg1	Enterobacteriaceae					1156:1173	unknown Enterobacteriaceae	1148:1173	unknown Enterobacteriaceae	1148:1173	Carnobacterium maltaromaticum, unknown Enterobacteriaceae, and Yersinia frederiksenii have genes for chitin and cellulose degradation, acetate, and glutamate production.					
37249588	6	62	theme	overwintering	938:950	arg1	period					952:957	late overwintering period	933:957	late overwintering period	933:957	Meanwhile, fatty acid degradation and betalain biosynthesis pathways are enriched in late overwintering period.					
37249588	4	63	theme	microbiomes	621:631	arg1	compositions					605:616	compositions	605:616	compositions of microbiomes with bias correction (ANCOM-BC)	605:663	Analysis of compositions of microbiomes with bias correction (ANCOM-BC) determined 15 Proteobacteria species enriched in late overwintering period.					
37249588	1	64	theme	migratory	191:199	arg1	birds					201:205	migratory birds	191:205	migratory birds	191:205	Less is known about the role of gut microbiota in overwintering environmental adaptation in migratory birds.					
37249588	8	65	contain	have	1203:1206	arg2	genes					1208:1212	genes	1208:1212	genes for chitin and cellulose degradation, acetate, and glutamate production	1208:1284	Carnobacterium maltaromaticum, unknown Enterobacteriaceae, and Yersinia frederiksenii have genes for chitin and cellulose degradation, acetate, and glutamate production.					
37249588	8	65	contain	have	1203:1206	arg1	frederiksenii					1189:1201	Yersinia frederiksenii	1180:1201	Yersinia frederiksenii	1180:1201	Carnobacterium maltaromaticum, unknown Enterobacteriaceae, and Yersinia frederiksenii have genes for chitin and cellulose degradation, acetate, and glutamate production.					
37249588	8	65	contain	have	1203:1206	arg1	maltaromaticum					1132:1145	Carnobacterium maltaromaticum	1117:1145	Carnobacterium maltaromaticum	1117:1145	Carnobacterium maltaromaticum, unknown Enterobacteriaceae, and Yersinia frederiksenii have genes for chitin and cellulose degradation, acetate, and glutamate production.					
37249588	8	65	contain	have	1203:1206	arg1	Enterobacteriaceae					1156:1173	unknown Enterobacteriaceae	1148:1173	unknown Enterobacteriaceae	1148:1173	Carnobacterium maltaromaticum, unknown Enterobacteriaceae, and Yersinia frederiksenii have genes for chitin and cellulose degradation, acetate, and glutamate production.					
37249588	12	66	theme	antibiotic	1953:1962	arg1	genes					1975:1979	antibiotic resistance genes	1953:1979	antibiotic resistance genes (ARGs)	1953:1986	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	12	66	theme	antibiotic	1953:1962	arg1	Proteobacteria					1878:1891	enriched Proteobacteria	1869:1891	enriched Proteobacteria	1869:1891	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	12	66	theme	antibiotic	1953:1962	arg1	ARGs					1982:1985	ARGs	1982:1985	ARGs	1982:1985	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	11	67	from	polysaccharide	1681:1694	arg1	diet					1705:1708	their diet	1699:1708	their diet	1699:1708	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	13	68	theme	core	2150:2153	arg1	abundance					2166:2174	core microbiota abundance	2150:2174	core microbiota abundance	2150:2174	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	13	69	theme	black-necked	2291:2302	arg1	cranes					2304:2309	black-necked cranes	2291:2309	black-necked cranes	2291:2309	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	6	70	theme	biosynthesis	895:906	arg1	pathways					908:915	betalain biosynthesis pathways	886:915	betalain biosynthesis pathways	886:915	Meanwhile, fatty acid degradation and betalain biosynthesis pathways are enriched in late overwintering period.					
37249588	7	71	theme	high-quality	1005:1016	arg1	bins					1018:1021	91 high-quality bins	1002:1021	91 high-quality bins (completeness >70% and contamination <10%)	1002:1064	Furthermore, metagenomic binning obtained 91 high-quality bins (completeness >70% and contamination <10%), 5 of which enriched in late overwintering period.					
37249588	13	72	theme	cranes	2115:2120	arg1	period					2092:2097	overwintering period	2078:2097	overwintering period of black-necked cranes	2078:2120	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	2	73	theme	fecal	259:263	arg1	n = 24					274:279	n = 24	274:279	n = 24	274:279	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	2	73	theme	fecal	259:263	arg1	samples					265:271	fresh fecal samples	253:271	fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late)	253:379	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	2	74	theme	overwintering	312:324	arg1	periods					301:307	4 periods	299:307	4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late)	299:379	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	3	75	theme	microbial	544:552	arg1	diversity					554:562	microbial diversity	544:562	microbial diversity among overwintering periods	544:590	The results demonstrated no significant change in microbial diversity among overwintering periods.					
37249588	9	76	theme	γ-aminobutyrate	1436:1450	arg1	production					1452:1461	γ-aminobutyrate production	1436:1461	γ-aminobutyrate production	1436:1461	Unknown Enterobacteriaceae and Y. frederiksenii hold genes for synthesis of 10 essential amino acids required by birds, and the latter has genes for γ-aminobutyrate production.					
37249588	5	77	theme	lipids	840:845	arg1	degradation					802:812	degradation	802:812	degradation of chitin, cellulose, and lipids	802:845	Based on previous reports, these species are associated with degradation of chitin, cellulose, and lipids.					
37249588	4	78	theme	bias	638:641	arg1	ANCOM-BC					655:662	ANCOM-BC	655:662	ANCOM-BC	655:662	Analysis of compositions of microbiomes with bias correction (ANCOM-BC) determined 15 Proteobacteria species enriched in late overwintering period.					
37249588	4	78	theme	bias	638:641	arg1	correction					643:652	bias correction	638:652	bias correction (ANCOM-BC)	638:663	Analysis of compositions of microbiomes with bias correction (ANCOM-BC) determined 15 Proteobacteria species enriched in late overwintering period.					
37249588	0	79	theme	impending	78:86	arg1	migration					88:96	impending migration	78:96	impending migration	78:96	Gut microbiota enhance energy accumulation of black-necked crane to cope with impending migration.					
37249588	13	80	theme	overwintering	2078:2090	arg1	period					2092:2097	overwintering period	2078:2097	overwintering period of black-necked cranes	2078:2120	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	1	81	theme	microbiota	135:144	arg1	role					123:126	the role	119:126	the role of gut microbiota in overwintering environmental adaptation in migratory birds	119:205	Less is known about the role of gut microbiota in overwintering environmental adaptation in migratory birds.					
37249588	2	82	theme	microbial	401:409	arg1	characteristics					436:450	microbial taxonomic and functional characteristics	401:450	microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis)	401:491	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	2	83	theme	black-necked	455:466	arg1	crane					468:472	black-necked crane	455:472	black-necked crane (Grus nigricollis)	455:491	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	4	84	theme	overwintering	719:731	arg1	period					733:738	late overwintering period	714:738	late overwintering period	714:738	Analysis of compositions of microbiomes with bias correction (ANCOM-BC) determined 15 Proteobacteria species enriched in late overwintering period.					
37249588	13	85	theme	gut	2047:2049	arg1	function					2062:2069	gut microbiota function	2047:2069	gut microbiota function during overwintering period of black-necked cranes	2047:2120	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	9	86	theme	Y.	1318:1319	arg1	frederiksenii					1321:1333	Y. frederiksenii	1318:1333	Y. frederiksenii	1318:1333	Unknown Enterobacteriaceae and Y. frederiksenii hold genes for synthesis of 10 essential amino acids required by birds, and the latter has genes for γ-aminobutyrate production.					
37249588	0	87	theme	energy	23:28	arg1	accumulation					30:41	energy accumulation	23:41	energy accumulation of black-necked crane	23:63	Gut microbiota enhance energy accumulation of black-necked crane to cope with impending migration.					
37249588	13	88	theme	overwintering	2242:2254	arg1	period					2256:2261	overwintering period	2242:2261	overwintering period • Gut microbiota	2242:2278	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	12	89	theme	enriched	1869:1876	arg1	genes					1975:1979	antibiotic resistance genes	1953:1979	antibiotic resistance genes (ARGs)	1953:1986	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	12	89	theme	enriched	1869:1876	arg1	enzymes					1931:1937	more carbohydrate-active enzymes	1906:1937	more carbohydrate-active enzymes (CAZymes)	1906:1947	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	12	89	theme	enriched	1869:1876	arg1	Proteobacteria					1878:1891	enriched Proteobacteria	1869:1891	enriched Proteobacteria	1869:1891	Additionally, enriched Proteobacteria also encoded more carbohydrate-active enzymes (CAZymes) and antibiotic resistance genes (ARGs) in late overwintering period.					
37249588	2	90	theme	metagenomic	227:237	arg1	sequencing					239:248	metagenomic sequencing	227:248	metagenomic sequencing	227:248	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	3	91	from	change	534:539	arg1	diversity					554:562	microbial diversity	544:562	microbial diversity among overwintering periods	544:590	The results demonstrated no significant change in microbial diversity among overwintering periods.					
37249588	13	92	theme	overwintering	2349:2361	arg1	period					2363:2368	the late overwintering period	2340:2368	the late overwintering period	2340:2368	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	7	93	theme	overwintering	1095:1107	arg1	period					1109:1114	late overwintering period	1090:1114	late overwintering period	1090:1114	Furthermore, metagenomic binning obtained 91 high-quality bins (completeness >70% and contamination <10%), 5 of which enriched in late overwintering period.					
37249588	2	94	dep	crane	468:472	arg1	nigricollis					480:490	Grus nigricollis	475:490	Grus nigricollis	475:490	Here, we performed metagenomic sequencing on fresh fecal samples (n = 24) collected during 4 periods of overwintering (Dec: early; Jan: middle I; Feb: middle II; Mar: late) to characterize gut microbial taxonomic and functional characteristics of black-necked crane (Grus nigricollis).					
37249588	8	95	theme	Yersinia	1180:1187	arg1	frederiksenii					1189:1201	Yersinia frederiksenii	1180:1201	Yersinia frederiksenii	1180:1201	Carnobacterium maltaromaticum, unknown Enterobacteriaceae, and Yersinia frederiksenii have genes for chitin and cellulose degradation, acetate, and glutamate production.					
37249588	0	96	theme	crane	59:63	arg1	accumulation					30:41	energy accumulation	23:41	energy accumulation of black-necked crane	23:63	Gut microbiota enhance energy accumulation of black-necked crane to cope with impending migration.					
37249588	11	97	theme	essential	1721:1729	arg1	acids					1737:1741	essential amino acids	1721:1741	essential amino acids	1721:1741	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	13	98	theme	•	2030:2030	arg1	Differences					2032:2042	• Differences	2030:2042	• Differences in gut microbiota function during overwintering period of black-necked cranes	2030:2120	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37249588	9	99	theme	essential	1366:1374	arg1	acids					1382:1386	10 essential amino acids	1363:1386	10 essential amino acids required by birds	1363:1404	Unknown Enterobacteriaceae and Y. frederiksenii hold genes for synthesis of 10 essential amino acids required by birds, and the latter has genes for γ-aminobutyrate production.					
37249588	10	100	theme	pyridoxal	1496:1504	arg1	synthesis					1506:1514	pyridoxal synthesis	1496:1514	pyridoxal synthesis	1496:1514	C. maltaromaticum has genes for pyridoxal synthesis.					
37249588	11	101	theme	black-necked	1599:1610	arg1	cranes					1612:1617	black-necked cranes	1599:1617	black-necked cranes	1599:1617	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	9	102	theme	acids	1382:1386	arg1	synthesis					1350:1358	synthesis	1350:1358	synthesis of 10 essential amino acids required by birds	1350:1404	Unknown Enterobacteriaceae and Y. frederiksenii hold genes for synthesis of 10 essential amino acids required by birds, and the latter has genes for γ-aminobutyrate production.					
37249588	7	103	dep	%	1040:1040	arg1	%					1063:1063	<10%	1060:1063	<10%	1060:1063	Furthermore, metagenomic binning obtained 91 high-quality bins (completeness >70% and contamination <10%), 5 of which enriched in late overwintering period.					
37249588	11	104	theme	energy	1636:1641	arg1	accumulation					1643:1654	pre-migratory energy accumulation	1622:1654	pre-migratory energy accumulation	1622:1654	These results implied the gut microbiota is adapted to the host diet and may help black-necked cranes in pre-migratory energy accumulation by degrading the complex polysaccharide in their diet, supplying essential amino acids and vitamin pyridoxal, and producing acetate, glutamate, and γ-aminobutyrate that could stimulate host feeding.					
37249588	13	105	theme	crane	2209:2213	arg1	microbiota					2182:2191	Gut microbiota	2178:2191	Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota	2178:2278	KEY POINTS: • Differences in gut microbiota function during overwintering period of black-necked cranes depend mainly on changes in core microbiota abundance • Gut microbiota of black-necked crane adapted to the diet during overwintering period • Gut microbiota could help black-necked cranes to accumulate more energy in the late overwintering period.					
37236561	5	0	theme	groups	661:666	arg1	content					632:638	The content	628:638	The content of phenolic hydroxyl groups in MAL	628:673	The content of phenolic hydroxyl groups in MAL significantly increased to 1.46 mmol/g.					
37236561	9	1	theme	treatment	1364:1372	arg1	applications					1374:1385	wastewater treatment applications	1353:1385	wastewater treatment applications	1353:1385	These results demonstrated the potential of MCGM for wastewater treatment applications.					
37236561	3	2	theme	groups	499:504	arg1	content					460:466	the content	456:466	the content of amine and phenolic hydroxyl groups	456:504	The factors influencing the modification conditions of the content of amine and phenolic hydroxyl groups were explored.					
37236561	8	3	from	number	1159:1164	arg1	Benefiting					1130:1139	Benefiting	1130:1139	Benefiting from a sufficient number of active sites, MCGM	1130:1186	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	2	4	theme	amine	338:342	arg1	groups					344:349	phenolic hydroxyl and amine groups	316:349	groups	344:349	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	2	5	from	rich	308:311	arg1	hydroxyl					325:332	phenolic hydroxyl and amine groups	316:349	hydroxyl	325:332	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	2	5	from	rich	308:311	arg1	groups					344:349	phenolic hydroxyl and amine groups	316:349	groups	344:349	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	7	6	theme	NaCMC	1045:1049	arg1	ratio					1056:1060	NaCMC mass ratio	1045:1060	NaCMC mass ratio	1045:1060	In addition, the effects of the MAL to NaCMC mass ratio, time, concentration, and pH on the adsorption of MB were explored.					
37236561	6	7	theme	sol-gel	904:910	arg1	process					912:918	a sol-gel process	902:918	a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents	902:1003	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	0	8	theme	blue	104:107	arg1	removal					109:115	methylene blue removal	94:115	methylene blue removal	94:115	Decoration of sodium carboxymethylcellulose gel microspheres with modified lignin to enhanced methylene blue removal.					
37236561	6	9	theme	adsorption	815:824	arg1	capacity					826:833	enhanced methylene blue (MB) adsorption capacity	786:833	enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL	786:880	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	2	10	theme	aminated	286:293	arg1	MAL					303:305	MAL	303:305	MAL	303:305	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	2	10	theme	aminated	286:293	arg1	lignin					295:300	modified aminated lignin	277:300	modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups	277:349	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	6	11	dep	blue	805:808	arg1	MB					811:812	MB	811:812	MB	811:812	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	8	12	theme	maximum	1255:1261	arg1	118.30 mg/g					1287:1297	118.30 mg/g	1287:1297	118.30 mg/g	1287:1297	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	8	12	theme	maximum	1255:1261	arg1	capacity					1274:1281	the maximum adsorption capacity	1251:1281	the maximum adsorption capacity	1251:1281	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	2	13	from	hydroxyl	325:332	arg1	rich					308:311	rich	308:311	rich	308:311	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	2	14	theme	modified	277:284	arg1	MAL					303:305	MAL	303:305	MAL	303:305	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	2	14	theme	modified	277:284	arg1	lignin					295:300	modified aminated lignin	277:300	modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups	277:349	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	2	15	theme	catalytic	381:389	arg1	grafting					391:398	catalytic grafting	381:398	catalytic grafting	381:398	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	6	16	with	microspheres	761:772	arg1	capacity					826:833	enhanced methylene blue (MB) adsorption capacity	786:833	enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL	786:880	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	9	17	theme	wastewater	1353:1362	arg1	applications					1374:1385	wastewater treatment applications	1353:1385	wastewater treatment applications	1353:1385	These results demonstrated the potential of MCGM for wastewater treatment applications.					
37236561	2	18	from	groups	344:349	arg1	rich					308:311	rich	308:311	rich	308:311	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	4	19	theme	two-step	611:618	arg1	method					620:625	a two-step method	609:625	a two-step method	609:625	Chemical structural analysis results confirmed that MAL was successfully prepared using a two-step method.					
37236561	6	20	theme	carboxymethylcellulose	726:747	arg1	microspheres					761:772	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres	715:772	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL	715:880	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	6	20	theme	carboxymethylcellulose	726:747	arg1	MCGM					775:778	MCGM	775:778	MCGM	775:778	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	5	21	theme	phenolic	643:650	arg1	groups					661:666	phenolic hydroxyl groups	643:666	phenolic hydroxyl groups	643:666	The content of phenolic hydroxyl groups in MAL significantly increased to 1.46 mmol/g.					
37236561	2	22	theme	rich	308:311	arg1	MAL					303:305	MAL	303:305	MAL	303:305	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	2	22	theme	rich	308:311	arg1	lignin					295:300	modified aminated lignin	277:300	modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups	277:349	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	6	23	theme	MAL/sodium	715:724	arg1	carboxymethylcellulose					726:747	MAL/sodium carboxymethylcellulose	715:747	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL	715:880	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	6	23	theme	MAL/sodium	715:724	arg1	NaCMC					750:754	NaCMC	750:754	NaCMC	750:754	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	1	24	theme	promising	187:195	arg1	introduction					122:133	The introduction	118:133	The introduction of active groups from biomass	118:163	The introduction of active groups from biomass is currently the most promising alternative method for increasing the adsorption effect of dyes.					
37236561	1	24	theme	promising	187:195	arg1	method					209:214	the most promising alternative method	178:214	the most promising alternative method for increasing the adsorption effect of dyes	178:259	The introduction of active groups from biomass is currently the most promising alternative method for increasing the adsorption effect of dyes.					
37236561	8	25	theme	sufficient	1148:1157	arg1	MCGM					1183:1186	MCGM	1183:1186	MCGM	1183:1186	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	8	25	theme	sufficient	1148:1157	arg1	number					1159:1164	a sufficient number	1146:1164	a sufficient number of active sites	1146:1180	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	6	26	theme	cross-linking	984:996	arg1	agents					998:1003	cross-linking agents	984:1003	cross-linking agents	984:1003	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	5	27	theme	hydroxyl	652:659	arg1	groups					661:666	phenolic hydroxyl groups	643:666	phenolic hydroxyl groups	643:666	The content of phenolic hydroxyl groups in MAL significantly increased to 1.46 mmol/g.					
37236561	6	28	theme	blue	805:808	arg1	capacity					826:833	enhanced methylene blue (MB) adsorption capacity	786:833	enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL	786:880	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	1	29	theme	alternative	197:207	arg1	introduction					122:133	The introduction	118:133	The introduction of active groups from biomass	118:163	The introduction of active groups from biomass is currently the most promising alternative method for increasing the adsorption effect of dyes.					
37236561	1	29	theme	alternative	197:207	arg1	method					209:214	the most promising alternative method	178:214	the most promising alternative method for increasing the adsorption effect of dyes	178:259	The introduction of active groups from biomass is currently the most promising alternative method for increasing the adsorption effect of dyes.					
37236561	0	30	theme	carboxymethylcellulose	21:42	arg1	microspheres					48:59	sodium carboxymethylcellulose gel microspheres	14:59	sodium carboxymethylcellulose gel microspheres with modified lignin	14:80	Decoration of sodium carboxymethylcellulose gel microspheres with modified lignin to enhanced methylene blue removal.					
37236561	8	31	theme	adsorption	1263:1272	arg1	118.30 mg/g					1287:1297	118.30 mg/g	1287:1297	118.30 mg/g	1287:1297	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	8	31	theme	adsorption	1263:1272	arg1	capacity					1274:1281	the maximum adsorption capacity	1251:1281	the maximum adsorption capacity	1251:1281	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	1	32	from	biomass	157:163	arg1	introduction					122:133	The introduction	118:133	The introduction of active groups from biomass	118:163	The introduction of active groups from biomass is currently the most promising alternative method for increasing the adsorption effect of dyes.					
37236561	1	32	from	biomass	157:163	arg1	method					209:214	the most promising alternative method	178:214	the most promising alternative method for increasing the adsorption effect of dyes	178:259	The introduction of active groups from biomass is currently the most promising alternative method for increasing the adsorption effect of dyes.					
37236561	3	33	theme	content	460:466	arg1	conditions					442:451	the modification conditions	425:451	the modification conditions of the content of amine and phenolic hydroxyl groups	425:504	The factors influencing the modification conditions of the content of amine and phenolic hydroxyl groups were explored.					
37236561	0	34	theme	sodium	14:19	arg1	microspheres					48:59	sodium carboxymethylcellulose gel microspheres	14:59	sodium carboxymethylcellulose gel microspheres with modified lignin	14:80	Decoration of sodium carboxymethylcellulose gel microspheres with modified lignin to enhanced methylene blue removal.					
37236561	5	35	from	content	632:638	arg1	MAL					671:673	MAL	671:673	MAL	671:673	The content of phenolic hydroxyl groups in MAL significantly increased to 1.46 mmol/g.					
37236561	0	36	theme	microspheres	48:59	arg1	Decoration					0:9	Decoration	0:9	Decoration of sodium carboxymethylcellulose gel microspheres with modified lignin to	0:83	Decoration of sodium carboxymethylcellulose gel microspheres with modified lignin to enhanced methylene blue removal.					
37236561	6	37	with	composite	863:871	arg1	MAL					878:880	MAL	878:880	MAL	878:880	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	7	38	theme	mass	1051:1054	arg1	ratio					1056:1060	NaCMC mass ratio	1045:1060	NaCMC mass ratio	1045:1060	In addition, the effects of the MAL to NaCMC mass ratio, time, concentration, and pH on the adsorption of MB were explored.					
37236561	8	39	theme	adsorption	1211:1220	arg1	capacity					1222:1229	an ultrahigh adsorption capacity	1198:1229	an ultrahigh adsorption capacity for MB removal	1198:1244	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	7	40	from	effects	1023:1029	arg1	adsorption					1098:1107	the adsorption	1094:1107	the adsorption of MB	1094:1113	In addition, the effects of the MAL to NaCMC mass ratio, time, concentration, and pH on the adsorption of MB were explored.					
37236561	0	41	theme	gel	44:46	arg1	microspheres					48:59	sodium carboxymethylcellulose gel microspheres	14:59	sodium carboxymethylcellulose gel microspheres with modified lignin	14:80	Decoration of sodium carboxymethylcellulose gel microspheres with modified lignin to enhanced methylene blue removal.					
37236561	4	42	theme	structural	530:539	arg1	results					550:556	Chemical structural analysis results	521:556	Chemical structural analysis results	521:556	Chemical structural analysis results confirmed that MAL was successfully prepared using a two-step method.					
37236561	8	43	theme	sites	1176:1180	arg1	MCGM					1183:1186	MCGM	1183:1186	MCGM	1183:1186	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	8	43	theme	sites	1176:1180	arg1	number					1159:1164	a sufficient number	1146:1164	a sufficient number of active sites	1146:1180	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	6	44	theme	composite	863:871	arg1	formation					848:856	the formation	844:856	the formation of a composite with MAL	844:880	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	8	45	theme	active	1169:1174	arg1	sites					1176:1180	active sites	1169:1180	active sites	1169:1180	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	6	46	theme	enhanced	786:793	arg1	capacity					826:833	enhanced methylene blue (MB) adsorption capacity	786:833	enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL	786:880	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	6	47	theme	freeze-drying	932:944	arg1	cations					968:974	freeze-drying and using multivalent cations	932:974	freeze-drying and using multivalent cations Al3+ as cross-linking agents	932:1003	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	0	48	theme	modified	66:73	arg1	lignin					75:80	modified lignin	66:80	modified lignin	66:80	Decoration of sodium carboxymethylcellulose gel microspheres with modified lignin to enhanced methylene blue removal.					
37236561	4	49	theme	Chemical	521:528	arg1	results					550:556	Chemical structural analysis results	521:556	Chemical structural analysis results	521:556	Chemical structural analysis results confirmed that MAL was successfully prepared using a two-step method.					
37236561	3	50	theme	modification	429:440	arg1	conditions					442:451	the modification conditions	425:451	the modification conditions of the content of amine and phenolic hydroxyl groups	425:504	The factors influencing the modification conditions of the content of amine and phenolic hydroxyl groups were explored.					
37236561	3	51	theme	phenolic	481:488	arg1	groups					499:504	phenolic hydroxyl groups	481:504	phenolic hydroxyl groups	481:504	The factors influencing the modification conditions of the content of amine and phenolic hydroxyl groups were explored.					
37236561	7	52	theme	MB	1112:1113	arg1	adsorption					1098:1107	the adsorption	1094:1107	the adsorption of MB	1094:1113	In addition, the effects of the MAL to NaCMC mass ratio, time, concentration, and pH on the adsorption of MB were explored.					
37236561	9	53	theme	MCGM	1344:1347	arg1	potential					1331:1339	the potential	1327:1339	the potential of MCGM for wastewater treatment applications	1327:1385	These results demonstrated the potential of MCGM for wastewater treatment applications.					
37236561	3	54	theme	amine	471:475	arg1	content					460:466	the content	456:466	the content of amine and phenolic hydroxyl groups	456:504	The factors influencing the modification conditions of the content of amine and phenolic hydroxyl groups were explored.					
37236561	3	55	theme	hydroxyl	490:497	arg1	groups					499:504	phenolic hydroxyl groups	481:504	phenolic hydroxyl groups	481:504	The factors influencing the modification conditions of the content of amine and phenolic hydroxyl groups were explored.					
37236561	4	56	theme	analysis	541:548	arg1	results					550:556	Chemical structural analysis results	521:556	Chemical structural analysis results	521:556	Chemical structural analysis results confirmed that MAL was successfully prepared using a two-step method.					
37236561	6	57	theme	multivalent	956:966	arg1	cations					968:974	freeze-drying and using multivalent cations	932:974	freeze-drying and using multivalent cations Al3+ as cross-linking agents	932:1003	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	1	58	theme	active	138:143	arg1	groups					145:150	active groups	138:150	active groups	138:150	The introduction of active groups from biomass is currently the most promising alternative method for increasing the adsorption effect of dyes.					
37236561	6	59	theme	gel	757:759	arg1	microspheres					761:772	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres	715:772	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL	715:880	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	6	59	theme	gel	757:759	arg1	MCGM					775:778	MCGM	775:778	MCGM	775:778	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	1	60	theme	adsorption	235:244	arg1	effect					246:251	the adsorption effect	231:251	the adsorption effect of dyes	231:259	The introduction of active groups from biomass is currently the most promising alternative method for increasing the adsorption effect of dyes.					
37236561	7	61	theme	MAL	1038:1040	arg1	effects					1023:1029	the effects	1019:1029	the effects of the MAL to NaCMC mass ratio, time, concentration, and pH on the adsorption of MB	1019:1113	In addition, the effects of the MAL to NaCMC mass ratio, time, concentration, and pH on the adsorption of MB were explored.					
37236561	1	62	theme	groups	145:150	arg1	introduction					122:133	The introduction	118:133	The introduction of active groups from biomass	118:163	The introduction of active groups from biomass is currently the most promising alternative method for increasing the adsorption effect of dyes.					
37236561	1	62	theme	groups	145:150	arg1	method					209:214	the most promising alternative method	178:214	the most promising alternative method for increasing the adsorption effect of dyes	178:259	The introduction of active groups from biomass is currently the most promising alternative method for increasing the adsorption effect of dyes.					
37236561	2	63	theme	phenolic	316:323	arg1	hydroxyl					325:332	phenolic hydroxyl and amine groups	316:349	hydroxyl	325:332	In this study, modified aminated lignin (MAL) rich in phenolic hydroxyl and amine groups was prepared by amination and catalytic grafting.					
37236561	8	64	theme	MB	1235:1236	arg1	removal					1238:1244	MB removal	1235:1244	MB removal	1235:1244	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	0	65	with	microspheres	48:59	arg1	lignin					75:80	modified lignin	66:80	modified lignin	66:80	Decoration of sodium carboxymethylcellulose gel microspheres with modified lignin to enhanced methylene blue removal.					
37236561	8	66	theme	ultrahigh	1201:1209	arg1	capacity					1222:1229	an ultrahigh adsorption capacity	1198:1229	an ultrahigh adsorption capacity for MB removal	1198:1244	Benefiting from a sufficient number of active sites, MCGM exhibited an ultrahigh adsorption capacity for MB removal, and the maximum adsorption capacity was 118.30 mg/g.					
37236561	6	67	theme	using	950:954	arg1	cations					968:974	freeze-drying and using multivalent cations	932:974	freeze-drying and using multivalent cations Al3+ as cross-linking agents	932:1003	MAL/sodium carboxymethylcellulose (NaCMC) gel microspheres (MCGM) with enhanced methylene blue (MB) adsorption capacity owing to the formation of a composite with MAL were synthesized by a sol-gel process followed by freeze-drying and using multivalent cations Al3+ as cross-linking agents.					
37236561	1	68	theme	dyes	256:259	arg1	effect					246:251	the adsorption effect	231:251	the adsorption effect of dyes	231:259	The introduction of active groups from biomass is currently the most promising alternative method for increasing the adsorption effect of dyes.					
35735135	13	0	theme	ratio	1893:1897	arg1	formulation					1913:1923	The 4APM-HAFU (ratio 1:5) hydrogel formulation	1878:1923	The 4APM-HAFU (ratio 1:5) hydrogel formulation	1878:1923	The 4APM-HAFU (ratio 1:5) hydrogel formulation showed sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation.					
35735135	12	1	theme	retro-Diels-Alder	1819:1835	arg1	reaction					1837:1844	the retro-Diels-Alder reaction	1815:1844	the retro-Diels-Alder reaction	1815:1844	Swelling and degradation studies showed that the hydrogels are biodegradable by the retro-Diels-Alder reaction under physiological conditions.					
35735135	11	2	theme	mechanical	1601:1610	arg1	properties					1612:1621	the mechanical properties	1597:1621	the mechanical properties of an ex vivo intravitreally formed hydrogel	1597:1666	In this study, the mechanical properties of an ex vivo intravitreally formed hydrogel were investigated and compared to the in vitro fabricated samples.					
35735135	13	3	theme	swelling	2007:2014	arg1	combination					1981:1991	a combination	1979:1991	a combination of diffusion, swelling, and degradation	1979:2031	The 4APM-HAFU (ratio 1:5) hydrogel formulation showed sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation.					
35735135	7	4	theme	intravitreal	853:864	arg1	injection					866:874	intravitreal injection	853:874	intravitreal injection	853:874	After intravitreal injection, the formulation is designed to undergo a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives.					
35735135	9	5	theme	effects	1252:1258	arg1	investigation					1231:1243	a systematic investigation	1218:1243	a systematic investigation of the effects of polymer composition	1218:1281	Here, a systematic investigation of the effects of polymer composition and the ratio between functional groups on the physicochemical properties of hydrogels was performed to select the most suitable formulation for protein delivery.					
35735135	9	5	theme	effects	1252:1258	arg1	ratio					1291:1295	the ratio	1287:1295	the ratio between functional groups on the physicochemical properties of hydrogels	1287:1368	Here, a systematic investigation of the effects of polymer composition and the ratio between functional groups on the physicochemical properties of hydrogels was performed to select the most suitable formulation for protein delivery.					
35735135	13	6	theme	1:5	1899:1901	arg1	formulation					1913:1923	The 4APM-HAFU (ratio 1:5) hydrogel formulation	1878:1923	The 4APM-HAFU (ratio 1:5) hydrogel formulation	1878:1923	The 4APM-HAFU (ratio 1:5) hydrogel formulation showed sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation.					
35735135	9	7	theme	polymer	1263:1269	arg1	composition					1271:1281	polymer composition	1263:1281	polymer composition	1263:1281	Here, a systematic investigation of the effects of polymer composition and the ratio between functional groups on the physicochemical properties of hydrogels was performed to select the most suitable formulation for protein delivery.					
35735135	2	8	theme	diseases	247:254	arg1	treatment					226:234	the treatment	222:234	the treatment of retinal diseases	222:254	The effectiveness of antibodies for the treatment of retinal diseases has been demonstrated.					
35735135	7	9	theme	bioactives	1043:1052	arg1	release					1032:1038	long-term sustained release	1012:1038	long-term sustained release of bioactives	1012:1052	After intravitreal injection, the formulation is designed to undergo a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives.					
35735135	15	10	theme	antibodies	2211:2220	arg1	release					2188:2194	the sustained release	2174:2194	the sustained release of therapeutic antibodies	2174:2220	The hydrogel platform described in this study offers high potential for the sustained release of therapeutic antibodies to treat ocular diseases.					
35735135	3	11	theme	high	332:335	arg1	concentrations					337:350	sufficiently high concentrations	319:350	sufficiently high concentrations of these protein therapeutics	319:380	Despite the clinical success, achieving sufficiently high concentrations of these protein therapeutics at the target tissue for an extended period is challenging.					
35735135	6	12	theme	small	827:831	arg1	29G					841:843	29G	841:843	29G	841:843	This study systematically characterized degradable "in situ" forming hydrogels that can be easily injected into the vitreous cavity using a small needle (29G).					
35735135	6	12	theme	small	827:831	arg1	needle					833:838	a small needle	825:838	a small needle (29G)	825:844	This study systematically characterized degradable "in situ" forming hydrogels that can be easily injected into the vitreous cavity using a small needle (29G).					
35735135	7	13	theme	long-term	1012:1020	arg1	release					1032:1038	long-term sustained release	1012:1038	long-term sustained release of bioactives	1012:1052	After intravitreal injection, the formulation is designed to undergo a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives.					
35735135	7	14	from	site	965:968	arg1	transition					932:941	a sol-gel phase transition	916:941	a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives	916:1052	After intravitreal injection, the formulation is designed to undergo a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives.					
35735135	3	15	theme	protein	361:367	arg1	therapeutics					369:380	these protein therapeutics	355:380	these protein therapeutics	355:380	Despite the clinical success, achieving sufficiently high concentrations of these protein therapeutics at the target tissue for an extended period is challenging.					
35735135	4	16	theme	macular	466:472	arg1	degeneration					474:485	macular degeneration	466:485	macular degeneration	466:485	Patients suffering from macular degeneration often receive injections once per month.					
35735135	0	17	theme	Sustained	68:76	arg1	Delivery					90:97	Sustained Intraocular Delivery	68:97	Sustained Intraocular Delivery of Bevacizumab	68:112	Hyaluronic Acid-PEG-Based Diels-Alder In Situ Forming Hydrogels for Sustained Intraocular Delivery of Bevacizumab.					
35735135	9	18	theme	functional	1305:1314	arg1	groups					1316:1321	functional groups	1305:1321	functional groups on the physicochemical properties of hydrogels	1305:1368	Here, a systematic investigation of the effects of polymer composition and the ratio between functional groups on the physicochemical properties of hydrogels was performed to select the most suitable formulation for protein delivery.					
35735135	1	19	theme	visual	157:162	arg1	worldwide					175:183	visual impairment worldwide	157:183	visual impairment worldwide	157:183	Retinal diseases are the leading cause of visual impairment worldwide.					
35735135	13	20	theme	>	1965:1965	arg1	days					1971:1974	bevacizumab > 400 days	1953:1974	bevacizumab > 400 days	1953:1974	The 4APM-HAFU (ratio 1:5) hydrogel formulation showed sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation.					
35735135	5	21	theme	injections	619:628	arg1	number					609:614	the number	605:614	the number of injections and adverse effects	605:648	Therefore, there is a growing need for suitable systems that can help reduce the number of injections and adverse effects while improving patient complacency.					
35735135	1	22	theme	worldwide	175:183	arg1	cause					148:152	the leading cause	136:152	the leading cause of visual impairment worldwide	136:183	Retinal diseases are the leading cause of visual impairment worldwide.					
35735135	1	22	theme	worldwide	175:183	arg1	diseases					123:130	Retinal diseases	115:130	Retinal diseases	115:130	Retinal diseases are the leading cause of visual impairment worldwide.					
35735135	3	23	theme	target	389:394	arg1	tissue					396:401	the target tissue	385:401	the target tissue for an extended period	385:424	Despite the clinical success, achieving sufficiently high concentrations of these protein therapeutics at the target tissue for an extended period is challenging.					
35735135	13	24	theme	days	1971:1974	arg1	release					1942:1948	sustained release	1932:1948	sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation	1932:2031	The 4APM-HAFU (ratio 1:5) hydrogel formulation showed sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation.					
35735135	0	25	theme	Bevacizumab	102:112	arg1	Delivery					90:97	Sustained Intraocular Delivery	68:97	Sustained Intraocular Delivery of Bevacizumab	68:112	Hyaluronic Acid-PEG-Based Diels-Alder In Situ Forming Hydrogels for Sustained Intraocular Delivery of Bevacizumab.					
35735135	9	26	theme	physicochemical	1330:1344	arg1	properties					1346:1355	the physicochemical properties	1326:1355	the physicochemical properties of hydrogels	1326:1368	Here, a systematic investigation of the effects of polymer composition and the ratio between functional groups on the physicochemical properties of hydrogels was performed to select the most suitable formulation for protein delivery.					
35735135	5	27	theme	adverse	634:640	arg1	effects					642:648	adverse effects	634:648	adverse effects	634:648	Therefore, there is a growing need for suitable systems that can help reduce the number of injections and adverse effects while improving patient complacency.					
35735135	6	28	theme	degradable	727:736	arg1	"					746:746	degradable "in situ"	727:746	degradable "in situ" forming hydrogels that can be easily injected into the vitreous cavity using a small needle (29G)	727:844	This study systematically characterized degradable "in situ" forming hydrogels that can be easily injected into the vitreous cavity using a small needle (29G).					
35735135	13	29	theme	sustained	1932:1940	arg1	release					1942:1948	sustained release	1932:1948	sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation	1932:2031	The 4APM-HAFU (ratio 1:5) hydrogel formulation showed sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation.					
35735135	14	30	theme	released	2061:2068	arg1	bevacizumab					2070:2080	the released bevacizumab	2057:2080	the released bevacizumab	2057:2080	A bioassay showed that the released bevacizumab remained bioactive.					
35735135	3	31	theme	extended	410:417	arg1	period					419:424	an extended period	407:424	an extended period	407:424	Despite the clinical success, achieving sufficiently high concentrations of these protein therapeutics at the target tissue for an extended period is challenging.					
35735135	10	32	theme	fastest	1509:1515	arg1	formation					1521:1529	the fastest gel formation	1505:1529	the fastest gel formation within 5 min after mixing the hydrogel precursors	1505:1579	Rheological analysis showed rapid hydrogel formation, with the fastest gel formation within 5 min after mixing the hydrogel precursors.					
35735135	0	33	theme	Hyaluronic	0:9	arg1	Diels-Alder					26:36	Hyaluronic Acid-PEG-Based Diels-Alder	0:36	Hyaluronic Acid-PEG-Based Diels-Alder In Situ	0:44	Hyaluronic Acid-PEG-Based Diels-Alder In Situ Forming Hydrogels for Sustained Intraocular Delivery of Bevacizumab.					
35735135	8	34	theme	acid-bearing	1116:1127	arg1	groups					1135:1140	hyaluronic acid-bearing furan groups	1105:1140	hyaluronic acid-bearing furan groups (HAFU)	1105:1147	A Diels-Alder reaction was exploited to crosslink hyaluronic acid-bearing furan groups (HAFU) with 4 arm-PEG10K-maleimide (4APM), yielding stable hydrogels.					
35735135	8	34	theme	acid-bearing	1116:1127	arg1	HAFU					1143:1146	HAFU	1143:1146	HAFU	1143:1146	A Diels-Alder reaction was exploited to crosslink hyaluronic acid-bearing furan groups (HAFU) with 4 arm-PEG10K-maleimide (4APM), yielding stable hydrogels.					
35735135	9	35	theme	suitable	1403:1410	arg1	formulation					1412:1422	the most suitable formulation	1394:1422	the most suitable formulation for protein delivery	1394:1443	Here, a systematic investigation of the effects of polymer composition and the ratio between functional groups on the physicochemical properties of hydrogels was performed to select the most suitable formulation for protein delivery.					
35735135	15	36	theme	sustained	2178:2186	arg1	release					2188:2194	the sustained release	2174:2194	the sustained release of therapeutic antibodies	2174:2220	The hydrogel platform described in this study offers high potential for the sustained release of therapeutic antibodies to treat ocular diseases.					
35735135	10	37	theme	rapid	1474:1478	arg1	formation					1489:1497	rapid hydrogel formation	1474:1497	rapid hydrogel formation	1474:1497	Rheological analysis showed rapid hydrogel formation, with the fastest gel formation within 5 min after mixing the hydrogel precursors.					
35735135	9	38	theme	systematic	1220:1229	arg1	investigation					1231:1243	a systematic investigation	1218:1243	a systematic investigation of the effects of polymer composition	1218:1281	Here, a systematic investigation of the effects of polymer composition and the ratio between functional groups on the physicochemical properties of hydrogels was performed to select the most suitable formulation for protein delivery.					
35735135	11	39	theme	ex	1629:1630	arg1	hydrogel					1659:1666	an ex vivo intravitreally formed hydrogel	1626:1666	an ex vivo intravitreally formed hydrogel	1626:1666	In this study, the mechanical properties of an ex vivo intravitreally formed hydrogel were investigated and compared to the in vitro fabricated samples.					
35735135	12	40	theme	physiological	1852:1864	arg1	conditions					1866:1875	physiological conditions	1852:1875	physiological conditions	1852:1875	Swelling and degradation studies showed that the hydrogels are biodegradable by the retro-Diels-Alder reaction under physiological conditions.					
35735135	2	41	theme	antibodies	207:216	arg1	effectiveness					190:202	The effectiveness	186:202	The effectiveness of antibodies for the treatment of retinal diseases	186:254	The effectiveness of antibodies for the treatment of retinal diseases has been demonstrated.					
35735135	7	42	theme	phase	926:930	arg1	transition					932:941	a sol-gel phase transition	916:941	a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives	916:1052	After intravitreal injection, the formulation is designed to undergo a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives.					
35735135	15	43	theme	high	2155:2158	arg1	potential					2160:2168	high potential	2155:2168	high potential for the sustained release of therapeutic antibodies to treat ocular diseases	2155:2245	The hydrogel platform described in this study offers high potential for the sustained release of therapeutic antibodies to treat ocular diseases.					
35735135	11	44	theme	formed	1652:1657	arg1	hydrogel					1659:1666	an ex vivo intravitreally formed hydrogel	1626:1666	an ex vivo intravitreally formed hydrogel	1626:1666	In this study, the mechanical properties of an ex vivo intravitreally formed hydrogel were investigated and compared to the in vitro fabricated samples.					
35735135	13	45	theme	degradation	2021:2031	arg1	combination					1981:1991	a combination	1979:1991	a combination of diffusion, swelling, and degradation	1979:2031	The 4APM-HAFU (ratio 1:5) hydrogel formulation showed sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation.					
35735135	11	46	theme	fabricated	1715:1724	arg1	samples					1726:1732	the in vitro fabricated samples	1702:1732	the in vitro fabricated samples	1702:1732	In this study, the mechanical properties of an ex vivo intravitreally formed hydrogel were investigated and compared to the in vitro fabricated samples.					
35735135	8	47	theme	stable	1194:1199	arg1	hydrogels					1201:1209	stable hydrogels	1194:1209	stable hydrogels	1194:1209	A Diels-Alder reaction was exploited to crosslink hyaluronic acid-bearing furan groups (HAFU) with 4 arm-PEG10K-maleimide (4APM), yielding stable hydrogels.					
35735135	11	48	dep	ex	1629:1630	arg1	vivo					1632:1635	vivo	1632:1635	vivo	1632:1635	In this study, the mechanical properties of an ex vivo intravitreally formed hydrogel were investigated and compared to the in vitro fabricated samples.					
35735135	5	49	theme	patient	666:672	arg1	complacency					674:684	patient complacency	666:684	patient complacency	666:684	Therefore, there is a growing need for suitable systems that can help reduce the number of injections and adverse effects while improving patient complacency.					
35735135	15	50	theme	ocular	2231:2236	arg1	diseases					2238:2245	ocular diseases	2231:2245	ocular diseases	2231:2245	The hydrogel platform described in this study offers high potential for the sustained release of therapeutic antibodies to treat ocular diseases.					
35735135	10	51	theme	Rheological	1446:1456	arg1	analysis					1458:1465	Rheological analysis	1446:1465	Rheological analysis	1446:1465	Rheological analysis showed rapid hydrogel formation, with the fastest gel formation within 5 min after mixing the hydrogel precursors.					
35735135	7	52	theme	intraocular	983:993	arg1	system					1001:1006	an intraocular depot system	980:1006	an intraocular depot system for long-term sustained release of bioactives	980:1052	After intravitreal injection, the formulation is designed to undergo a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives.					
35735135	15	53	theme	therapeutic	2199:2209	arg1	antibodies					2211:2220	therapeutic antibodies	2199:2220	therapeutic antibodies	2199:2220	The hydrogel platform described in this study offers high potential for the sustained release of therapeutic antibodies to treat ocular diseases.					
35735135	3	54	theme	clinical	291:298	arg1	success					300:306	the clinical success	287:306	the clinical success	287:306	Despite the clinical success, achieving sufficiently high concentrations of these protein therapeutics at the target tissue for an extended period is challenging.					
35735135	9	55	theme	composition	1271:1281	arg1	effects					1252:1258	the effects	1248:1258	the effects of polymer composition	1248:1281	Here, a systematic investigation of the effects of polymer composition and the ratio between functional groups on the physicochemical properties of hydrogels was performed to select the most suitable formulation for protein delivery.					
35735135	2	56	theme	retinal	239:245	arg1	diseases					247:254	retinal diseases	239:254	retinal diseases	239:254	The effectiveness of antibodies for the treatment of retinal diseases has been demonstrated.					
35735135	9	57	from	groups	1316:1321	arg1	properties					1346:1355	the physicochemical properties	1326:1355	the physicochemical properties of hydrogels	1326:1368	Here, a systematic investigation of the effects of polymer composition and the ratio between functional groups on the physicochemical properties of hydrogels was performed to select the most suitable formulation for protein delivery.					
35735135	1	58	theme	leading	140:146	arg1	cause					148:152	the leading cause	136:152	the leading cause of visual impairment worldwide	136:183	Retinal diseases are the leading cause of visual impairment worldwide.					
35735135	1	58	theme	leading	140:146	arg1	diseases					123:130	Retinal diseases	115:130	Retinal diseases	115:130	Retinal diseases are the leading cause of visual impairment worldwide.					
35735135	12	59	theme	degradation	1748:1758	arg1	studies					1760:1766	Swelling and degradation studies	1735:1766	Swelling and degradation studies	1735:1766	Swelling and degradation studies showed that the hydrogels are biodegradable by the retro-Diels-Alder reaction under physiological conditions.					
35735135	0	60	theme	Intraocular	78:88	arg1	Delivery					90:97	Sustained Intraocular Delivery	68:97	Sustained Intraocular Delivery of Bevacizumab	68:112	Hyaluronic Acid-PEG-Based Diels-Alder In Situ Forming Hydrogels for Sustained Intraocular Delivery of Bevacizumab.					
35735135	7	61	theme	sustained	1022:1030	arg1	release					1032:1038	long-term sustained release	1012:1038	long-term sustained release of bioactives	1012:1052	After intravitreal injection, the formulation is designed to undergo a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives.					
35735135	3	62	theme	therapeutics	369:380	arg1	concentrations					337:350	sufficiently high concentrations	319:350	sufficiently high concentrations of these protein therapeutics	319:380	Despite the clinical success, achieving sufficiently high concentrations of these protein therapeutics at the target tissue for an extended period is challenging.					
35735135	13	63	theme	diffusion	1996:2004	arg1	combination					1981:1991	a combination	1979:1991	a combination of diffusion, swelling, and degradation	1979:2031	The 4APM-HAFU (ratio 1:5) hydrogel formulation showed sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation.					
35735135	13	64	theme	bevacizumab	1953:1963	arg1	days					1971:1974	bevacizumab > 400 days	1953:1974	bevacizumab > 400 days	1953:1974	The 4APM-HAFU (ratio 1:5) hydrogel formulation showed sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation.					
35735135	8	65	theme	Diels-Alder	1057:1067	arg1	reaction					1069:1076	A Diels-Alder reaction	1055:1076	A Diels-Alder reaction	1055:1076	A Diels-Alder reaction was exploited to crosslink hyaluronic acid-bearing furan groups (HAFU) with 4 arm-PEG10K-maleimide (4APM), yielding stable hydrogels.					
35735135	5	66	theme	suitable	567:574	arg1	systems					576:582	suitable systems	567:582	suitable systems that can help reduce the number of injections and adverse effects while improving patient complacency	567:684	Therefore, there is a growing need for suitable systems that can help reduce the number of injections and adverse effects while improving patient complacency.					
35735135	1	67	theme	impairment	164:173	arg1	worldwide					175:183	visual impairment worldwide	157:183	visual impairment worldwide	157:183	Retinal diseases are the leading cause of visual impairment worldwide.					
35735135	7	68	theme	depot	995:999	arg1	system					1001:1006	an intraocular depot system	980:1006	an intraocular depot system for long-term sustained release of bioactives	980:1052	After intravitreal injection, the formulation is designed to undergo a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives.					
35735135	12	69	theme	Swelling	1735:1742	arg1	studies					1760:1766	Swelling and degradation studies	1735:1766	Swelling and degradation studies	1735:1766	Swelling and degradation studies showed that the hydrogels are biodegradable by the retro-Diels-Alder reaction under physiological conditions.					
35735135	10	70	theme	hydrogel	1480:1487	arg1	formation					1489:1497	rapid hydrogel formation	1474:1497	rapid hydrogel formation	1474:1497	Rheological analysis showed rapid hydrogel formation, with the fastest gel formation within 5 min after mixing the hydrogel precursors.					
35735135	6	71	dep	in	739:740	arg1	situ					742:745	situ	742:745	situ	742:745	This study systematically characterized degradable "in situ" forming hydrogels that can be easily injected into the vitreous cavity using a small needle (29G).					
35735135	5	72	theme	effects	642:648	arg1	number					609:614	the number	605:614	the number of injections and adverse effects	605:648	Therefore, there is a growing need for suitable systems that can help reduce the number of injections and adverse effects while improving patient complacency.					
35735135	10	73	theme	gel	1517:1519	arg1	formation					1521:1529	the fastest gel formation	1505:1529	the fastest gel formation within 5 min after mixing the hydrogel precursors	1505:1579	Rheological analysis showed rapid hydrogel formation, with the fastest gel formation within 5 min after mixing the hydrogel precursors.					
35735135	0	74	theme	Acid-PEG-Based	11:24	arg1	Diels-Alder					26:36	Hyaluronic Acid-PEG-Based Diels-Alder	0:36	Hyaluronic Acid-PEG-Based Diels-Alder In Situ	0:44	Hyaluronic Acid-PEG-Based Diels-Alder In Situ Forming Hydrogels for Sustained Intraocular Delivery of Bevacizumab.					
35735135	9	75	theme	hydrogels	1360:1368	arg1	properties					1346:1355	the physicochemical properties	1326:1355	the physicochemical properties of hydrogels	1326:1368	Here, a systematic investigation of the effects of polymer composition and the ratio between functional groups on the physicochemical properties of hydrogels was performed to select the most suitable formulation for protein delivery.					
35735135	8	76	theme	hyaluronic	1105:1114	arg1	groups					1135:1140	hyaluronic acid-bearing furan groups	1105:1140	hyaluronic acid-bearing furan groups (HAFU)	1105:1147	A Diels-Alder reaction was exploited to crosslink hyaluronic acid-bearing furan groups (HAFU) with 4 arm-PEG10K-maleimide (4APM), yielding stable hydrogels.					
35735135	8	76	theme	hyaluronic	1105:1114	arg1	HAFU					1143:1146	HAFU	1143:1146	HAFU	1143:1146	A Diels-Alder reaction was exploited to crosslink hyaluronic acid-bearing furan groups (HAFU) with 4 arm-PEG10K-maleimide (4APM), yielding stable hydrogels.					
35735135	15	77	theme	hydrogel	2106:2113	arg1	platform					2115:2122	The hydrogel platform	2102:2122	The hydrogel platform described in this study	2102:2146	The hydrogel platform described in this study offers high potential for the sustained release of therapeutic antibodies to treat ocular diseases.					
35735135	6	78	theme	in	739:740	arg1	"					746:746	degradable "in situ"	727:746	degradable "in situ" forming hydrogels that can be easily injected into the vitreous cavity using a small needle (29G)	727:844	This study systematically characterized degradable "in situ" forming hydrogels that can be easily injected into the vitreous cavity using a small needle (29G).					
35735135	0	79	theme	In	38:39	arg1	Diels-Alder					26:36	Hyaluronic Acid-PEG-Based Diels-Alder	0:36	Hyaluronic Acid-PEG-Based Diels-Alder In Situ	0:44	Hyaluronic Acid-PEG-Based Diels-Alder In Situ Forming Hydrogels for Sustained Intraocular Delivery of Bevacizumab.					
35735135	8	80	theme	furan	1129:1133	arg1	groups					1135:1140	hyaluronic acid-bearing furan groups	1105:1140	hyaluronic acid-bearing furan groups (HAFU)	1105:1147	A Diels-Alder reaction was exploited to crosslink hyaluronic acid-bearing furan groups (HAFU) with 4 arm-PEG10K-maleimide (4APM), yielding stable hydrogels.					
35735135	8	80	theme	furan	1129:1133	arg1	HAFU					1143:1146	HAFU	1143:1146	HAFU	1143:1146	A Diels-Alder reaction was exploited to crosslink hyaluronic acid-bearing furan groups (HAFU) with 4 arm-PEG10K-maleimide (4APM), yielding stable hydrogels.					
35735135	7	81	theme	administration	950:963	arg1	site					965:968	the administration site	946:968	the administration site	946:968	After intravitreal injection, the formulation is designed to undergo a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives.					
35735135	1	82	theme	Retinal	115:121	arg1	cause					148:152	the leading cause	136:152	the leading cause of visual impairment worldwide	136:183	Retinal diseases are the leading cause of visual impairment worldwide.					
35735135	1	82	theme	Retinal	115:121	arg1	diseases					123:130	Retinal diseases	115:130	Retinal diseases	115:130	Retinal diseases are the leading cause of visual impairment worldwide.					
35735135	13	83	theme	hydrogel	1904:1911	arg1	formulation					1913:1923	The 4APM-HAFU (ratio 1:5) hydrogel formulation	1878:1923	The 4APM-HAFU (ratio 1:5) hydrogel formulation	1878:1923	The 4APM-HAFU (ratio 1:5) hydrogel formulation showed sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation.					
35735135	6	84	theme	vitreous	803:810	arg1	cavity					812:817	the vitreous cavity	799:817	the vitreous cavity using a small needle (29G)	799:844	This study systematically characterized degradable "in situ" forming hydrogels that can be easily injected into the vitreous cavity using a small needle (29G).					
35735135	10	85	theme	hydrogel	1561:1568	arg1	precursors					1570:1579	the hydrogel precursors	1557:1579	the hydrogel precursors	1557:1579	Rheological analysis showed rapid hydrogel formation, with the fastest gel formation within 5 min after mixing the hydrogel precursors.					
35735135	0	86	dep	In	38:39	arg1	Situ					41:44	Situ	41:44	Situ	41:44	Hyaluronic Acid-PEG-Based Diels-Alder In Situ Forming Hydrogels for Sustained Intraocular Delivery of Bevacizumab.					
35735135	11	87	theme	hydrogel	1659:1666	arg1	properties					1612:1621	the mechanical properties	1597:1621	the mechanical properties of an ex vivo intravitreally formed hydrogel	1597:1666	In this study, the mechanical properties of an ex vivo intravitreally formed hydrogel were investigated and compared to the in vitro fabricated samples.					
35735135	7	88	theme	sol-gel	918:924	arg1	transition					932:941	a sol-gel phase transition	916:941	a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives	916:1052	After intravitreal injection, the formulation is designed to undergo a sol-gel phase transition at the administration site to obtain an intraocular depot system for long-term sustained release of bioactives.					
35735135	9	89	theme	protein	1428:1434	arg1	delivery					1436:1443	protein delivery	1428:1443	protein delivery	1428:1443	Here, a systematic investigation of the effects of polymer composition and the ratio between functional groups on the physicochemical properties of hydrogels was performed to select the most suitable formulation for protein delivery.					
35735135	13	90	theme	4APM-HAFU	1882:1890	arg1	formulation					1913:1923	The 4APM-HAFU (ratio 1:5) hydrogel formulation	1878:1923	The 4APM-HAFU (ratio 1:5) hydrogel formulation	1878:1923	The 4APM-HAFU (ratio 1:5) hydrogel formulation showed sustained release of bevacizumab > 400 days by a combination of diffusion, swelling, and degradation.					
35735135	5	91	theme	growing	550:556	arg1	need					558:561	a growing need	548:561	a growing need for suitable systems that can help reduce the number of injections and adverse effects while improving patient complacency	548:684	Therefore, there is a growing need for suitable systems that can help reduce the number of injections and adverse effects while improving patient complacency.					
36001455	7	0	theme	tumor	1276:1280	arg1	region					1282:1287	the tumor region	1272:1287	the tumor region	1272:1287	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	6	1	theme	therapeutic	1053:1063	arg1	agent					1065:1069	the final three-component therapeutic agent	1027:1069	the final three-component therapeutic agent	1027:1069	When the nanocomposite is activated by local laser exposure, the final three-component therapeutic agent is demonstrated to present targeted photothermal conversion capability and chemodynamic activity together with chemotherapy.					
36001455	2	2	theme	effect	396:401	arg1	combination					403:413	the effect combination	392:413	the effect combination of additional components	392:438	The security and targeting of therapeutic agents also require the effect combination of additional components.					
36001455	6	3	theme	final	1031:1035	arg1	agent					1065:1069	the final three-component therapeutic agent	1027:1069	the final three-component therapeutic agent	1027:1069	When the nanocomposite is activated by local laser exposure, the final three-component therapeutic agent is demonstrated to present targeted photothermal conversion capability and chemodynamic activity together with chemotherapy.					
36001455	0	4	theme	Chemodynamic	114:125	arg1	Therapy					127:133	Chemodynamic Therapy	114:133	Chemodynamic Therapy	114:133	Hyaluronic Acid-Enwrapped Polyoxometalate Complex for Synergistic Near Infrared-II Photothermal/Chemo-Therapy and Chemodynamic Therapy.					
36001455	3	5	from	construction	454:465	arg1	system					509:514	a simple system	500:514	a simple system with intelligent cooperative responsiveness	500:558	However, the construction of multifunctional composites in a simple system with intelligent cooperative responsiveness remains a challenge.					
36001455	8	6	from	photothermal/chemo-therapy	1537:1562	arg1	development					1496:1506	the development	1492:1506	the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy	1492:1599	This work presents an opportunity for the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy.					
36001455	5	7	theme	host-guest	937:946	arg1	inclusion					948:956	host-guest inclusion	937:956	host-guest inclusion to DOX	937:963	To reduce the physiological toxicity, hyaluronic acid grafting β-cyclodextrin side chains is used to cover the ionic complex through host-guest inclusion to DOX.					
36001455	6	8	theme	photothermal	1107:1118	arg1	capability					1131:1140	targeted photothermal conversion capability	1098:1140	targeted photothermal conversion capability	1098:1140	When the nanocomposite is activated by local laser exposure, the final three-component therapeutic agent is demonstrated to present targeted photothermal conversion capability and chemodynamic activity together with chemotherapy.					
36001455	7	9	theme	oxygen	1433:1438	arg1	1O2					1449:1451	1O2	1449:1451	1O2	1449:1451	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	7	9	theme	oxygen	1433:1438	arg1	species					1440:1446	toxic reactive oxygen species	1418:1446	toxic reactive oxygen species	1418:1446	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	7	10	from	acidity	1261:1267	arg1	region					1282:1287	the tumor region	1272:1287	the tumor region	1272:1287	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	4	11	theme	ionic	785:789	arg1	interaction					791:801	ionic interaction	785:801	ionic interaction	785:801	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	2	12	theme	therapeutic	360:370	arg1	agents					372:377	therapeutic agents	360:377	therapeutic agents	360:377	The security and targeting of therapeutic agents also require the effect combination of additional components.					
36001455	7	13	theme	mild	1256:1259	arg1	acidity					1261:1267	mild acidity	1256:1267	mild acidity in the tumor region	1256:1287	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	5	14	theme	side	882:885	arg1	chains					887:892	β-cyclodextrin side chains	867:892	β-cyclodextrin side chains	867:892	To reduce the physiological toxicity, hyaluronic acid grafting β-cyclodextrin side chains is used to cover the ionic complex through host-guest inclusion to DOX.					
36001455	3	15	theme	multifunctional	470:484	arg1	composites					486:495	multifunctional composites	470:495	multifunctional composites	470:495	However, the construction of multifunctional composites in a simple system with intelligent cooperative responsiveness remains a challenge.					
36001455	1	16	theme	functional	196:205	arg1	components					207:216	functional components	196:216	functional components into a targeting system	196:240	To enhance the efficacy of tumor therapy, the collection of functional components into a targeting system shows advantages over most homogeneous materials in inducing apoptosis of cancer cells.					
36001455	7	17	theme	acidity	1261:1267	arg1	stimulation					1241:1251	the stimulation	1237:1251	the stimulation of mild acidity in the tumor region	1237:1287	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	7	18	from	stimulation	1241:1251	arg1	region					1282:1287	the tumor region	1272:1287	the tumor region	1272:1287	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	7	19	theme	controlled	1205:1214	arg1	release					1216:1222	the controlled release	1201:1222	the controlled release of DOX under the stimulation of mild acidity in the tumor region	1201:1287	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	1	20	theme	most	264:267	arg1	materials					281:289	most homogeneous materials	264:289	most homogeneous materials	264:289	To enhance the efficacy of tumor therapy, the collection of functional components into a targeting system shows advantages over most homogeneous materials in inducing apoptosis of cancer cells.					
36001455	6	21	theme	chemodynamic	1146:1157	arg1	activity					1159:1166	chemodynamic activity	1146:1166	chemodynamic activity	1146:1166	When the nanocomposite is activated by local laser exposure, the final three-component therapeutic agent is demonstrated to present targeted photothermal conversion capability and chemodynamic activity together with chemotherapy.					
36001455	1	22	theme	components	207:216	arg1	collection					182:191	the collection	178:191	the collection of functional components into a targeting system	178:240	To enhance the efficacy of tumor therapy, the collection of functional components into a targeting system shows advantages over most homogeneous materials in inducing apoptosis of cancer cells.					
36001455	0	23	theme	Acid-Enwrapped	11:24	arg1	Complex					42:48	Hyaluronic Acid-Enwrapped Polyoxometalate Complex	0:48	Hyaluronic Acid-Enwrapped Polyoxometalate Complex for Synergistic Near Infrared-II Photothermal/Chemo-Therapy and Chemodynamic Therapy.	0:134	Hyaluronic Acid-Enwrapped Polyoxometalate Complex for Synergistic Near Infrared-II Photothermal/Chemo-Therapy and Chemodynamic Therapy.					
36001455	7	24	with	aroused	1336:1342	arg1	effect					1306:1311	photothermal effect	1293:1311	photothermal effect	1293:1311	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	7	24	with	aroused	1336:1342	arg1	release					1216:1222	the controlled release	1201:1222	the controlled release of DOX under the stimulation of mild acidity in the tumor region	1201:1287	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	6	25	theme	laser	1011:1015	arg1	exposure					1017:1024	local laser exposure	1005:1024	local laser exposure	1005:1024	When the nanocomposite is activated by local laser exposure, the final three-component therapeutic agent is demonstrated to present targeted photothermal conversion capability and chemodynamic activity together with chemotherapy.					
36001455	2	26	theme	components	429:438	arg1	combination					403:413	the effect combination	392:413	the effect combination of additional components	392:438	The security and targeting of therapeutic agents also require the effect combination of additional components.					
36001455	0	27	theme	Hyaluronic	0:9	arg1	Complex					42:48	Hyaluronic Acid-Enwrapped Polyoxometalate Complex	0:48	Hyaluronic Acid-Enwrapped Polyoxometalate Complex for Synergistic Near Infrared-II Photothermal/Chemo-Therapy and Chemodynamic Therapy.	0:134	Hyaluronic Acid-Enwrapped Polyoxometalate Complex for Synergistic Near Infrared-II Photothermal/Chemo-Therapy and Chemodynamic Therapy.					
36001455	1	28	theme	cancer	316:321	arg1	cells					323:327	cancer cells	316:327	cancer cells	316:327	To enhance the efficacy of tumor therapy, the collection of functional components into a targeting system shows advantages over most homogeneous materials in inducing apoptosis of cancer cells.					
36001455	4	29	theme	doxorubicin	745:755	arg1	DOX					772:774	DOX	772:774	DOX	772:774	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	4	29	theme	doxorubicin	745:755	arg1	hydrochloride					757:769	doxorubicin hydrochloride	745:769	the positively charged doxorubicin hydrochloride (DOX)	722:775	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	5	30	theme	hyaluronic	842:851	arg1	acid					853:856	hyaluronic acid	842:856	hyaluronic acid grafting β-cyclodextrin side chains	842:892	To reduce the physiological toxicity, hyaluronic acid grafting β-cyclodextrin side chains is used to cover the ionic complex through host-guest inclusion to DOX.					
36001455	8	31	from	development	1496:1506	arg1	photothermal/chemo-therapy					1537:1562	NIR-II photothermal/chemo-therapy	1530:1562	NIR-II photothermal/chemo-therapy	1530:1562	This work presents an opportunity for the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy.					
36001455	8	31	from	development	1496:1506	arg1	therapy					1593:1599	chemodynamic synergistic therapy	1568:1599	chemodynamic synergistic therapy	1568:1599	This work presents an opportunity for the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy.					
36001455	2	32	theme	additional	418:427	arg1	components					429:438	additional components	418:438	additional components	418:438	The security and targeting of therapeutic agents also require the effect combination of additional components.					
36001455	4	33	theme	near	658:661	arg1	region					681:686	the near infrared (NIR) II region	654:686	the near infrared (NIR) II region	654:686	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	1	34	theme	cells	323:327	arg1	apoptosis					303:311	apoptosis	303:311	apoptosis of cancer cells	303:327	To enhance the efficacy of tumor therapy, the collection of functional components into a targeting system shows advantages over most homogeneous materials in inducing apoptosis of cancer cells.					
36001455	8	35	theme	nanocomposite	1513:1525	arg1	development					1496:1506	the development	1492:1506	the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy	1492:1599	This work presents an opportunity for the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy.					
36001455	7	36	theme	tumor	1384:1388	arg1	microenvironment					1390:1405	a tumor microenvironment	1382:1405	a tumor microenvironment	1382:1405	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	4	37	from	region	681:686	arg1	absorption					640:649	the absorption	636:649	the absorption at the near infrared (NIR) II region	636:686	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	1	38	theme	targeting	225:233	arg1	system					235:240	a targeting system	223:240	a targeting system	223:240	To enhance the efficacy of tumor therapy, the collection of functional components into a targeting system shows advantages over most homogeneous materials in inducing apoptosis of cancer cells.					
36001455	0	39	theme	Polyoxometalate	26:40	arg1	Complex					42:48	Hyaluronic Acid-Enwrapped Polyoxometalate Complex	0:48	Hyaluronic Acid-Enwrapped Polyoxometalate Complex for Synergistic Near Infrared-II Photothermal/Chemo-Therapy and Chemodynamic Therapy.	0:134	Hyaluronic Acid-Enwrapped Polyoxometalate Complex for Synergistic Near Infrared-II Photothermal/Chemo-Therapy and Chemodynamic Therapy.					
36001455	3	40	with	system	509:514	arg1	responsiveness					545:558	intelligent cooperative responsiveness	521:558	intelligent cooperative responsiveness	521:558	However, the construction of multifunctional composites in a simple system with intelligent cooperative responsiveness remains a challenge.					
36001455	5	41	used	used	897:900	arg2	acid					853:856	hyaluronic acid	842:856	hyaluronic acid grafting β-cyclodextrin side chains	842:892	To reduce the physiological toxicity, hyaluronic acid grafting β-cyclodextrin side chains is used to cover the ionic complex through host-guest inclusion to DOX.					
36001455	3	42	theme	intelligent	521:531	arg1	responsiveness					545:558	intelligent cooperative responsiveness	521:558	intelligent cooperative responsiveness	521:558	However, the construction of multifunctional composites in a simple system with intelligent cooperative responsiveness remains a challenge.					
36001455	0	43	theme	Synergistic	54:64	arg1	Photothermal/Chemo-Therapy					83:108	Synergistic Near Infrared-II Photothermal/Chemo-Therapy	54:108	Synergistic Near Infrared-II Photothermal/Chemo-Therapy	54:108	Hyaluronic Acid-Enwrapped Polyoxometalate Complex for Synergistic Near Infrared-II Photothermal/Chemo-Therapy and Chemodynamic Therapy.					
36001455	4	44	theme	core	701:704	arg1	carrier					706:712	a core carrier	699:712	a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction	699:801	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	4	44	theme	core	701:704	arg1	cluster					611:617	a reduced polyanionic cluster	589:617	a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region	589:686	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	7	45	theme	hydrogen	1347:1354	arg1	peroxide					1356:1363	hydrogen peroxide	1347:1363	hydrogen peroxide overexpressed in a tumor microenvironment	1347:1405	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	3	46	theme	composites	486:495	arg1	construction					454:465	the construction	450:465	the construction of multifunctional composites in a simple system with intelligent cooperative responsiveness	450:558	However, the construction of multifunctional composites in a simple system with intelligent cooperative responsiveness remains a challenge.					
36001455	3	47	theme	simple	502:507	arg1	system					509:514	a simple system	500:514	a simple system with intelligent cooperative responsiveness	500:558	However, the construction of multifunctional composites in a simple system with intelligent cooperative responsiveness remains a challenge.					
36001455	7	48	theme	DOX	1227:1229	arg1	effect					1306:1311	photothermal effect	1293:1311	photothermal effect	1293:1311	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	7	48	theme	DOX	1227:1229	arg1	release					1216:1222	the controlled release	1201:1222	the controlled release of DOX under the stimulation of mild acidity in the tumor region	1201:1287	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	4	49	theme	reduced	591:597	arg1	rP2W18					620:625	rP2W18	620:625	rP2W18	620:625	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	4	49	theme	reduced	591:597	arg1	carrier					706:712	a core carrier	699:712	a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction	699:801	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	4	49	theme	reduced	591:597	arg1	cluster					611:617	a reduced polyanionic cluster	589:617	a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region	589:686	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	4	50	theme	NIR	673:675	arg1	region					681:686	the near infrared (NIR) II region	654:686	the near infrared (NIR) II region	654:686	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	8	51	from	nanocomposite	1513:1525	arg1	photothermal/chemo-therapy					1537:1562	NIR-II photothermal/chemo-therapy	1530:1562	NIR-II photothermal/chemo-therapy	1530:1562	This work presents an opportunity for the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy.					
36001455	8	51	from	nanocomposite	1513:1525	arg1	therapy					1593:1599	chemodynamic synergistic therapy	1568:1599	chemodynamic synergistic therapy	1568:1599	This work presents an opportunity for the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy.					
36001455	6	52	theme	conversion	1120:1129	arg1	capability					1131:1140	targeted photothermal conversion capability	1098:1140	targeted photothermal conversion capability	1098:1140	When the nanocomposite is activated by local laser exposure, the final three-component therapeutic agent is demonstrated to present targeted photothermal conversion capability and chemodynamic activity together with chemotherapy.					
36001455	0	53	theme	Infrared-II	71:81	arg1	Photothermal/Chemo-Therapy					83:108	Synergistic Near Infrared-II Photothermal/Chemo-Therapy	54:108	Synergistic Near Infrared-II Photothermal/Chemo-Therapy	54:108	Hyaluronic Acid-Enwrapped Polyoxometalate Complex for Synergistic Near Infrared-II Photothermal/Chemo-Therapy and Chemodynamic Therapy.					
36001455	6	54	theme	local	1005:1009	arg1	exposure					1017:1024	local laser exposure	1005:1024	local laser exposure	1005:1024	When the nanocomposite is activated by local laser exposure, the final three-component therapeutic agent is demonstrated to present targeted photothermal conversion capability and chemodynamic activity together with chemotherapy.					
36001455	8	55	theme	synergistic	1581:1591	arg1	therapy					1593:1599	chemodynamic synergistic therapy	1568:1599	chemodynamic synergistic therapy	1568:1599	This work presents an opportunity for the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy.					
36001455	4	56	theme	charged	737:743	arg1	DOX					772:774	DOX	772:774	DOX	772:774	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	4	56	theme	charged	737:743	arg1	hydrochloride					757:769	doxorubicin hydrochloride	745:769	the positively charged doxorubicin hydrochloride (DOX)	722:775	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	0	57	theme	Near	66:69	arg1	Photothermal/Chemo-Therapy					83:108	Synergistic Near Infrared-II Photothermal/Chemo-Therapy	54:108	Synergistic Near Infrared-II Photothermal/Chemo-Therapy	54:108	Hyaluronic Acid-Enwrapped Polyoxometalate Complex for Synergistic Near Infrared-II Photothermal/Chemo-Therapy and Chemodynamic Therapy.					
36001455	3	58	theme	cooperative	533:543	arg1	responsiveness					545:558	intelligent cooperative responsiveness	521:558	intelligent cooperative responsiveness	521:558	However, the construction of multifunctional composites in a simple system with intelligent cooperative responsiveness remains a challenge.					
36001455	8	59	theme	NIR-II	1530:1535	arg1	photothermal/chemo-therapy					1537:1562	NIR-II photothermal/chemo-therapy	1530:1562	NIR-II photothermal/chemo-therapy	1530:1562	This work presents an opportunity for the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy.					
36001455	4	60	used	used	691:694	arg2	carrier					706:712	a core carrier	699:712	a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction	699:801	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	4	60	used	used	691:694	arg2	rP2W18					620:625	rP2W18	620:625	rP2W18	620:625	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	4	60	used	used	691:694	arg2	cluster					611:617	a reduced polyanionic cluster	589:617	a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region	589:686	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	2	61	dep	security	334:341	arg1	The					330:332	The	330:332	The	330:332	The security and targeting of therapeutic agents also require the effect combination of additional components.					
36001455	4	62	theme	polyanionic	599:609	arg1	rP2W18					620:625	rP2W18	620:625	rP2W18	620:625	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	4	62	theme	polyanionic	599:609	arg1	carrier					706:712	a core carrier	699:712	a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction	699:801	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	4	62	theme	polyanionic	599:609	arg1	cluster					611:617	a reduced polyanionic cluster	589:617	a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region	589:686	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	7	63	from	region	1282:1287	arg1	stimulation					1241:1251	the stimulation	1237:1251	the stimulation of mild acidity in the tumor region	1237:1287	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	7	64	theme	reactive	1424:1431	arg1	1O2					1449:1451	1O2	1449:1451	1O2	1449:1451	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	7	64	theme	reactive	1424:1431	arg1	species					1440:1446	toxic reactive oxygen species	1418:1446	toxic reactive oxygen species	1418:1446	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	1	65	theme	tumor	163:167	arg1	therapy					169:175	tumor therapy	163:175	tumor therapy	163:175	To enhance the efficacy of tumor therapy, the collection of functional components into a targeting system shows advantages over most homogeneous materials in inducing apoptosis of cancer cells.					
36001455	4	66	theme	infrared	663:670	arg1	region					681:686	the near infrared (NIR) II region	654:686	the near infrared (NIR) II region	654:686	Herein, a reduced polyanionic cluster (rP2W18) bearing the absorption at the near infrared (NIR) II region is used as a core carrier to bind the positively charged doxorubicin hydrochloride (DOX) through ionic interaction.					
36001455	8	67	theme	chemodynamic	1568:1579	arg1	therapy					1593:1599	chemodynamic synergistic therapy	1568:1599	chemodynamic synergistic therapy	1568:1599	This work presents an opportunity for the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy.					
36001455	6	68	theme	targeted	1098:1105	arg1	capability					1131:1140	targeted photothermal conversion capability	1098:1140	targeted photothermal conversion capability	1098:1140	When the nanocomposite is activated by local laser exposure, the final three-component therapeutic agent is demonstrated to present targeted photothermal conversion capability and chemodynamic activity together with chemotherapy.					
36001455	1	69	theme	therapy	169:175	arg1	efficacy					151:158	the efficacy	147:158	the efficacy of tumor therapy	147:175	To enhance the efficacy of tumor therapy, the collection of functional components into a targeting system shows advantages over most homogeneous materials in inducing apoptosis of cancer cells.					
36001455	2	70	theme	agents	372:377	arg1	targeting					347:355	targeting	347:355	targeting	347:355	The security and targeting of therapeutic agents also require the effect combination of additional components.					
36001455	2	70	theme	agents	372:377	arg1	security					334:341	security	334:341	security	334:341	The security and targeting of therapeutic agents also require the effect combination of additional components.					
36001455	8	71	from	therapy	1593:1599	arg1	development					1496:1506	the development	1492:1506	the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy	1492:1599	This work presents an opportunity for the development of a nanocomposite in NIR-II photothermal/chemo-therapy and chemodynamic synergistic therapy.					
36001455	5	72	theme	β-cyclodextrin	867:880	arg1	chains					887:892	β-cyclodextrin side chains	867:892	β-cyclodextrin side chains	867:892	To reduce the physiological toxicity, hyaluronic acid grafting β-cyclodextrin side chains is used to cover the ionic complex through host-guest inclusion to DOX.					
36001455	5	73	theme	ionic	915:919	arg1	complex					921:927	the ionic complex	911:927	the ionic complex	911:927	To reduce the physiological toxicity, hyaluronic acid grafting β-cyclodextrin side chains is used to cover the ionic complex through host-guest inclusion to DOX.					
36001455	7	74	theme	photothermal	1293:1304	arg1	effect					1306:1311	photothermal effect	1293:1311	photothermal effect	1293:1311	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	1	75	theme	homogeneous	269:279	arg1	materials					281:289	most homogeneous materials	264:289	most homogeneous materials	264:289	To enhance the efficacy of tumor therapy, the collection of functional components into a targeting system shows advantages over most homogeneous materials in inducing apoptosis of cancer cells.					
36001455	5	76	theme	physiological	818:830	arg1	toxicity					832:839	the physiological toxicity	814:839	the physiological toxicity	814:839	To reduce the physiological toxicity, hyaluronic acid grafting β-cyclodextrin side chains is used to cover the ionic complex through host-guest inclusion to DOX.					
36001455	7	77	theme	toxic	1418:1422	arg1	1O2					1449:1451	1O2	1449:1451	1O2	1449:1451	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	7	77	theme	toxic	1418:1422	arg1	species					1440:1446	toxic reactive oxygen species	1418:1446	toxic reactive oxygen species	1418:1446	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	7	78	theme	exposed	1318:1324	arg1	aroused					1336:1342	aroused	1336:1342	aroused	1336:1342	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	7	78	theme	exposed	1318:1324	arg1	rP2W18					1326:1331	the exposed rP2W18	1314:1331	the exposed rP2W18	1314:1331	With the controlled release of DOX under the stimulation of mild acidity in the tumor region and photothermal effect, the exposed rP2W18 is aroused by hydrogen peroxide overexpressed in a tumor microenvironment to produce toxic reactive oxygen species, 1O2.					
36001455	6	79	theme	three-component	1037:1051	arg1	agent					1065:1069	the final three-component therapeutic agent	1027:1069	the final three-component therapeutic agent	1027:1069	When the nanocomposite is activated by local laser exposure, the final three-component therapeutic agent is demonstrated to present targeted photothermal conversion capability and chemodynamic activity together with chemotherapy.					
36241261	0	0	theme	composite	89:97	arg1	beads					99:103	composite beads	89:103	composite beads	89:103	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides composite beads.					
36241261	3	1	theme	pH	485:486	arg1	effects					474:480	The effects	470:480	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics	470:602	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics were studied.					
36241261	8	2	theme	composite	1152:1160	arg1	adsorbent					1187:1195	adsorbent	1187:1195	adsorbent	1187:1195	Therefore, the cellulose/LDHs composite beads can be a promising adsorbent for removing micro/nanoplastics.					
36241261	8	2	theme	composite	1152:1160	arg1	beads					1162:1166	the cellulose/LDHs composite beads	1133:1166	the cellulose/LDHs composite beads	1133:1166	Therefore, the cellulose/LDHs composite beads can be a promising adsorbent for removing micro/nanoplastics.					
36241261	0	3	from	removal	10:16	arg1	water					39:43	water	39:43	water	39:43	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides composite beads.					
36241261	6	4	theme	diffusion	1009:1017	arg1	model					1020:1024	Webber-Morris (intra-particle diffusion) model	979:1024	Webber-Morris (intra-particle diffusion) model	979:1024	The attachment behavior can be successfully explained using the pseudo-second-order kinetic model (R2 = 0.964), Webber-Morris (intra-particle diffusion) model, and Langmuir isotherm model (R2 = 0.978).					
36241261	6	5	theme	Langmuir	1031:1038	arg1	R2 = 0.978					1056:1065	R2 = 0.978	1056:1065	R2 = 0.978	1056:1065	The attachment behavior can be successfully explained using the pseudo-second-order kinetic model (R2 = 0.964), Webber-Morris (intra-particle diffusion) model, and Langmuir isotherm model (R2 = 0.978).					
36241261	6	5	theme	Langmuir	1031:1038	arg1	model					1049:1053	Langmuir isotherm model	1031:1053	Langmuir isotherm model (R2 = 0.978)	1031:1066	The attachment behavior can be successfully explained using the pseudo-second-order kinetic model (R2 = 0.964), Webber-Morris (intra-particle diffusion) model, and Langmuir isotherm model (R2 = 0.978).					
36241261	2	6	theme	double	282:287	arg1	beads					317:321	cellulose/MgAl layered double hydroxides (LDHs) composite beads	259:321	cellulose/MgAl layered double hydroxides (LDHs) composite beads	259:321	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	6	7	theme	intra-particle	994:1007	arg1	model					1020:1024	Webber-Morris (intra-particle diffusion) model	979:1024	Webber-Morris (intra-particle diffusion) model	979:1024	The attachment behavior can be successfully explained using the pseudo-second-order kinetic model (R2 = 0.964), Webber-Morris (intra-particle diffusion) model, and Langmuir isotherm model (R2 = 0.978).					
36241261	2	8	theme	layered	274:280	arg1	beads					317:321	cellulose/MgAl layered double hydroxides (LDHs) composite beads	259:321	cellulose/MgAl layered double hydroxides (LDHs) composite beads	259:321	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	3	9	theme	acid	545:548	arg1	effects					474:480	The effects	470:480	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics	470:602	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics were studied.					
36241261	2	10	theme	cellulose/MgAl	259:272	arg1	beads					317:321	cellulose/MgAl layered double hydroxides (LDHs) composite beads	259:321	cellulose/MgAl layered double hydroxides (LDHs) composite beads	259:321	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	4	11	theme	microstructure	622:635	arg1	characteristics					637:651	The microstructure characteristics	618:651	The microstructure characteristics of the beads	618:664	The microstructure characteristics of the beads were also analyzed before and after the attachment of nanoplastics.					
36241261	0	12	from	water	39:43	arg1	nanoplastics					21:32	nanoplastics	21:32	nanoplastics from water	21:43	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides composite beads.					
36241261	0	12	from	water	39:43	arg1	removal					10:16	Effective removal	0:16	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides	0:87	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides composite beads.					
36241261	0	13	dep	beads	99:103	arg1	removal					10:16	Effective removal	0:16	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides	0:87	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides composite beads.					
36241261	2	14	theme	unique	438:443	arg1	charge					454:459	the unique positive charge	434:459	the unique positive charge of LDHs	434:467	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	6	15	theme	attachment	871:880	arg1	behavior					882:889	The attachment behavior	867:889	The attachment behavior	867:889	The attachment behavior can be successfully explained using the pseudo-second-order kinetic model (R2 = 0.964), Webber-Morris (intra-particle diffusion) model, and Langmuir isotherm model (R2 = 0.978).					
36241261	7	16	theme	attachment	1081:1090	arg1	capacity					1092:1099	The maximum attachment capacity	1069:1099	The maximum attachment capacity	1069:1099	The maximum attachment capacity can reach 6.08 mg/g.					
36241261	8	17	theme	cellulose/LDHs	1137:1150	arg1	adsorbent					1187:1195	adsorbent	1187:1195	adsorbent	1187:1195	Therefore, the cellulose/LDHs composite beads can be a promising adsorbent for removing micro/nanoplastics.					
36241261	8	17	theme	cellulose/LDHs	1137:1150	arg1	beads					1162:1166	the cellulose/LDHs composite beads	1133:1166	the cellulose/LDHs composite beads	1133:1166	Therefore, the cellulose/LDHs composite beads can be a promising adsorbent for removing micro/nanoplastics.					
36241261	3	18	theme	humic	539:543	arg1	acid					545:548	humic acid	539:548	humic acid	539:548	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics were studied.					
36241261	2	19	theme	nanoparticles	368:380	arg1	removal					345:351	the removal	341:351	the removal of polystyrene nanoparticles	341:380	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	0	20	theme	Effective	0:8	arg1	removal					10:16	Effective removal	0:16	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides	0:87	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides composite beads.					
36241261	6	21	theme	Webber-Morris	979:991	arg1	model					1020:1024	Webber-Morris (intra-particle diffusion) model	979:1024	Webber-Morris (intra-particle diffusion) model	979:1024	The attachment behavior can be successfully explained using the pseudo-second-order kinetic model (R2 = 0.964), Webber-Morris (intra-particle diffusion) model, and Langmuir isotherm model (R2 = 0.978).					
36241261	3	22	from	effects	474:480	arg1	attachment					577:586	the attachment	573:586	the attachment of nanoplastics	573:602	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics were studied.					
36241261	2	23	theme	LDHs	301:304	arg1	beads					317:321	cellulose/MgAl layered double hydroxides (LDHs) composite beads	259:321	cellulose/MgAl layered double hydroxides (LDHs) composite beads	259:321	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	2	24	theme	polystyrene	356:366	arg1	nanoparticles					368:380	polystyrene nanoparticles	356:380	polystyrene nanoparticles	356:380	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	0	25	theme	nanoplastics	21:32	arg1	removal					10:16	Effective removal	0:16	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides	0:87	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides composite beads.					
36241261	5	26	theme	pore	801:804	arg1	diffusion					806:814	pore diffusion	801:814	pore diffusion	801:814	The results indicate that nanoplastic attachment probably involves pore diffusion, hydrogen bonding, and electrostatic interactions.					
36241261	1	27	theme	Micro/nanoplastic	106:122	arg1	concern					149:155	an emerging concern	137:155	an emerging concern	137:155	Micro/nanoplastic pollution is an emerging concern all over the world as it has a certain impact on the eco-environment and human health.					
36241261	1	27	theme	Micro/nanoplastic	106:122	arg1	pollution					124:132	Micro/nanoplastic pollution	106:132	Micro/nanoplastic pollution	106:132	Micro/nanoplastic pollution is an emerging concern all over the world as it has a certain impact on the eco-environment and human health.					
36241261	1	28	theme	certain	188:194	arg1	impact					196:201	a certain impact	186:201	a certain impact on the eco-environment and human health	186:241	Micro/nanoplastic pollution is an emerging concern all over the world as it has a certain impact on the eco-environment and human health.					
36241261	2	29	theme	cellulose	420:428	arg1	charge					454:459	the unique positive charge	434:459	the unique positive charge of LDHs	434:467	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	2	29	theme	cellulose	420:428	arg1	properties					406:415	the porous properties	395:415	the porous properties of cellulose	395:428	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	7	30	theme	maximum	1073:1079	arg1	capacity					1092:1099	The maximum attachment capacity	1069:1099	The maximum attachment capacity	1069:1099	The maximum attachment capacity can reach 6.08 mg/g.					
36241261	2	31	theme	hydroxides	289:298	arg1	beads					317:321	cellulose/MgAl layered double hydroxides (LDHs) composite beads	259:321	cellulose/MgAl layered double hydroxides (LDHs) composite beads	259:321	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	4	32	theme	beads	660:664	arg1	characteristics					637:651	The microstructure characteristics	618:651	The microstructure characteristics of the beads	618:664	The microstructure characteristics of the beads were also analyzed before and after the attachment of nanoplastics.					
36241261	2	33	theme	LDHs	464:467	arg1	charge					454:459	the unique positive charge	434:459	the unique positive charge of LDHs	434:467	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	2	33	theme	LDHs	464:467	arg1	properties					406:415	the porous properties	395:415	the porous properties of cellulose	395:428	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	1	34	contain	has	182:184	arg2	impact					196:201	a certain impact	186:201	a certain impact on the eco-environment and human health	186:241	Micro/nanoplastic pollution is an emerging concern all over the world as it has a certain impact on the eco-environment and human health.					
36241261	1	34	contain	has	182:184	arg1	it					179:180	it	179:180	it	179:180	Micro/nanoplastic pollution is an emerging concern all over the world as it has a certain impact on the eco-environment and human health.					
36241261	3	35	theme	ionic	555:559	arg1	strength					561:568	ionic strength	555:568	ionic strength	555:568	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics were studied.					
36241261	2	36	theme	porous	399:404	arg1	properties					406:415	the porous properties	395:415	the porous properties of cellulose	395:428	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	5	37	theme	nanoplastic	760:770	arg1	attachment					772:781	nanoplastic attachment	760:781	nanoplastic attachment	760:781	The results indicate that nanoplastic attachment probably involves pore diffusion, hydrogen bonding, and electrostatic interactions.					
36241261	3	38	theme	nanoplastics	591:602	arg1	attachment					577:586	the attachment	573:586	the attachment of nanoplastics	573:602	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics were studied.					
36241261	3	39	theme	strength	561:568	arg1	effects					474:480	The effects	470:480	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics	470:602	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics were studied.					
36241261	4	40	theme	nanoplastics	720:731	arg1	attachment					706:715	the attachment	702:715	the attachment of nanoplastics	702:731	The microstructure characteristics of the beads were also analyzed before and after the attachment of nanoplastics.					
36241261	0	41	theme	cellulose/MgAl	48:61	arg1	hydroxides					78:87	cellulose/MgAl layered double hydroxides	48:87	cellulose/MgAl layered double hydroxides	48:87	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides composite beads.					
36241261	2	42	theme	positive	445:452	arg1	charge					454:459	the unique positive charge	434:459	the unique positive charge of LDHs	434:467	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	6	43	theme	kinetic	951:957	arg1	model					959:963	the pseudo-second-order kinetic model	927:963	the pseudo-second-order kinetic model (R2 = 0.964)	927:976	The attachment behavior can be successfully explained using the pseudo-second-order kinetic model (R2 = 0.964), Webber-Morris (intra-particle diffusion) model, and Langmuir isotherm model (R2 = 0.978).					
36241261	6	43	theme	kinetic	951:957	arg1	R2 = 0.964					966:975	R2 = 0.964	966:975	R2 = 0.964	966:975	The attachment behavior can be successfully explained using the pseudo-second-order kinetic model (R2 = 0.964), Webber-Morris (intra-particle diffusion) model, and Langmuir isotherm model (R2 = 0.978).					
36241261	6	44	theme	pseudo-second-order	931:949	arg1	model					959:963	the pseudo-second-order kinetic model	927:963	the pseudo-second-order kinetic model (R2 = 0.964)	927:976	The attachment behavior can be successfully explained using the pseudo-second-order kinetic model (R2 = 0.964), Webber-Morris (intra-particle diffusion) model, and Langmuir isotherm model (R2 = 0.978).					
36241261	6	44	theme	pseudo-second-order	931:949	arg1	R2 = 0.964					966:975	R2 = 0.964	966:975	R2 = 0.964	966:975	The attachment behavior can be successfully explained using the pseudo-second-order kinetic model (R2 = 0.964), Webber-Morris (intra-particle diffusion) model, and Langmuir isotherm model (R2 = 0.978).					
36241261	1	45	from	impact	196:201	arg1	health					236:241	the eco-environment and human health	206:241	the eco-environment and human health	206:241	Micro/nanoplastic pollution is an emerging concern all over the world as it has a certain impact on the eco-environment and human health.					
36241261	1	46	theme	emerging	140:147	arg1	concern					149:155	an emerging concern	137:155	an emerging concern	137:155	Micro/nanoplastic pollution is an emerging concern all over the world as it has a certain impact on the eco-environment and human health.					
36241261	1	46	theme	emerging	140:147	arg1	pollution					124:132	Micro/nanoplastic pollution	106:132	Micro/nanoplastic pollution	106:132	Micro/nanoplastic pollution is an emerging concern all over the world as it has a certain impact on the eco-environment and human health.					
36241261	3	47	theme	temperature	526:536	arg1	effects					474:480	The effects	470:480	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics	470:602	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics were studied.					
36241261	1	48	theme	eco-environment	210:224	arg1	health					236:241	the eco-environment and human health	206:241	the eco-environment and human health	206:241	Micro/nanoplastic pollution is an emerging concern all over the world as it has a certain impact on the eco-environment and human health.					
36241261	3	49	theme	initial	503:509	arg1	concentration					511:523	initial concentration	503:523	initial concentration	503:523	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics were studied.					
36241261	3	50	theme	concentration	511:523	arg1	effects					474:480	The effects	470:480	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics	470:602	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics were studied.					
36241261	5	51	theme	electrostatic	839:851	arg1	interactions					853:864	electrostatic interactions	839:864	electrostatic interactions	839:864	The results indicate that nanoplastic attachment probably involves pore diffusion, hydrogen bonding, and electrostatic interactions.					
36241261	2	52	theme	composite	307:315	arg1	beads					317:321	cellulose/MgAl layered double hydroxides (LDHs) composite beads	259:321	cellulose/MgAl layered double hydroxides (LDHs) composite beads	259:321	In this study, cellulose/MgAl layered double hydroxides (LDHs) composite beads were prepared for the removal of polystyrene nanoparticles by utilizing the porous properties of cellulose and the unique positive charge of LDHs.					
36241261	0	53	theme	double	71:76	arg1	hydroxides					78:87	cellulose/MgAl layered double hydroxides	48:87	cellulose/MgAl layered double hydroxides	48:87	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides composite beads.					
36241261	6	54	theme	isotherm	1040:1047	arg1	R2 = 0.978					1056:1065	R2 = 0.978	1056:1065	R2 = 0.978	1056:1065	The attachment behavior can be successfully explained using the pseudo-second-order kinetic model (R2 = 0.964), Webber-Morris (intra-particle diffusion) model, and Langmuir isotherm model (R2 = 0.978).					
36241261	6	54	theme	isotherm	1040:1047	arg1	model					1049:1053	Langmuir isotherm model	1031:1053	Langmuir isotherm model (R2 = 0.978)	1031:1066	The attachment behavior can be successfully explained using the pseudo-second-order kinetic model (R2 = 0.964), Webber-Morris (intra-particle diffusion) model, and Langmuir isotherm model (R2 = 0.978).					
36241261	5	55	theme	hydrogen	817:824	arg1	bonding					826:832	hydrogen bonding	817:832	hydrogen bonding	817:832	The results indicate that nanoplastic attachment probably involves pore diffusion, hydrogen bonding, and electrostatic interactions.					
36241261	3	56	theme	contact	489:495	arg1	time					497:500	contact time	489:500	contact time	489:500	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics were studied.					
36241261	1	57	theme	human	230:234	arg1	health					236:241	the eco-environment and human health	206:241	the eco-environment and human health	206:241	Micro/nanoplastic pollution is an emerging concern all over the world as it has a certain impact on the eco-environment and human health.					
36241261	0	58	theme	layered	63:69	arg1	hydroxides					78:87	cellulose/MgAl layered double hydroxides	48:87	cellulose/MgAl layered double hydroxides	48:87	Effective removal of nanoplastics from water by cellulose/MgAl layered double hydroxides composite beads.					
36241261	8	59	theme	removing	1201:1208	arg1	micro/nanoplastics					1210:1227	removing micro/nanoplastics	1201:1227	removing micro/nanoplastics	1201:1227	Therefore, the cellulose/LDHs composite beads can be a promising adsorbent for removing micro/nanoplastics.					
36241261	3	60	theme	time	497:500	arg1	effects					474:480	The effects	470:480	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics	470:602	The effects of pH, contact time, initial concentration, temperature, humic acid, and ionic strength on the attachment of nanoplastics were studied.					
36195035	0	0	theme	components	86:95	arg1	distribution					53:64	the distribution	49:64	the distribution of cell wall matrix components in rice root cells	49:114	Microcystin-LR and cyanobacterial extracts alter the distribution of cell wall matrix components in rice root cells.					
36195035	5	1	theme	Semi-thin	728:736	arg1	sections					749:756	Semi-thin transverse sections	728:756	Semi-thin transverse sections of variously treated LR-White-embedded roots	728:801	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	6	2	theme	pectin	1044:1049	arg1	PME					1067:1069	PME	1067:1069	PME	1067:1069	Homogalacturonan and arabinan distribution patterns were altered in the affected roots, while a pectin methylesterase (PME) activity assay revealed that PMEs were also affected.					
36195035	6	2	theme	pectin	1044:1049	arg1	methylesterase					1051:1064	pectin methylesterase	1044:1064	a pectin methylesterase (PME) activity assay	1042:1085	Homogalacturonan and arabinan distribution patterns were altered in the affected roots, while a pectin methylesterase (PME) activity assay revealed that PMEs were also affected.					
36195035	4	3	theme	pure	555:558	arg1	microcystin-LR					560:573	pure microcystin-LR	555:573	pure microcystin-LR (MC-LR)	555:581	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	4	3	theme	pure	555:558	arg1	MC-LR					576:580	MC-LR	576:580	MC-LR	576:580	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	0	4	theme	matrix	79:84	arg1	components					86:95	cell wall matrix components	69:95	cell wall matrix components	69:95	Microcystin-LR and cyanobacterial extracts alter the distribution of cell wall matrix components in rice root cells.					
36195035	7	5	dep	along	1162:1166	arg1	with					1168:1171	with	1168:1171	with	1168:1171	Elevated intracellular Ca2+ levels, along with increased callose and mixed linkage glucans (MLGs) deposition, were also observed after treatment.					
36195035	0	6	theme	rice	100:103	arg1	cells					110:114	rice root cells	100:114	rice root cells	100:114	Microcystin-LR and cyanobacterial extracts alter the distribution of cell wall matrix components in rice root cells.					
36195035	3	7	from	effects	392:398	arg1	wall					441:444	plant cell wall	430:444	plant cell wall	430:444	However, the effects of cyanobacterial toxicity on plant cell wall have not been yet thoroughly studied.					
36195035	1	8	dep	toxins	132:137	arg1	known					140:144	known	140:144	known as cyanotoxins	140:159	Cyanobacterial toxins (known as cyanotoxins) disrupt the plant cytoskeleton (i.e. microtubules and F-actin), which is implicated in the regulation of cell wall architecture.					
36195035	7	9	theme	linkage	1201:1207	arg1	MLGs					1218:1221	MLGs	1218:1221	MLGs	1218:1221	Elevated intracellular Ca2+ levels, along with increased callose and mixed linkage glucans (MLGs) deposition, were also observed after treatment.					
36195035	7	9	theme	linkage	1201:1207	arg1	glucans					1209:1215	mixed linkage glucans	1195:1215	mixed linkage glucans (MLGs)	1195:1222	Elevated intracellular Ca2+ levels, along with increased callose and mixed linkage glucans (MLGs) deposition, were also observed after treatment.					
36195035	9	10	theme	exact	1344:1348	arg1	mechanism					1350:1358	The exact mechanism	1340:1358	The exact mechanism of cyanobacterial toxicity against the cell wall	1340:1407	The exact mechanism of cyanobacterial toxicity against the cell wall is to be further investigated.					
36195035	7	11	theme	callose	1183:1189	arg1	deposition					1224:1233	increased callose and mixed linkage glucans (MLGs) deposition	1173:1233	increased callose and mixed linkage glucans (MLGs) deposition	1173:1233	Elevated intracellular Ca2+ levels, along with increased callose and mixed linkage glucans (MLGs) deposition, were also observed after treatment.					
36195035	0	12	from	distribution	53:64	arg1	cells					110:114	rice root cells	100:114	rice root cells	100:114	Microcystin-LR and cyanobacterial extracts alter the distribution of cell wall matrix components in rice root cells.					
36195035	5	13	theme	various	832:838	arg1	hemicelluloses					932:945	hemicelluloses	932:945	hemicelluloses	932:945	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	5	13	theme	various	832:838	arg1	arabinogalactan-proteins					895:918	arabinogalactan-proteins	895:918	arabinogalactan-proteins (AGPs)	895:925	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	5	13	theme	various	832:838	arg1	epitopes					850:857	various cell wall epitopes	832:857	various cell wall epitopes	832:857	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	5	13	theme	various	832:838	arg1	homogalacturonans					870:886	homogalacturonans	870:886	homogalacturonans (HGs)	870:892	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	4	14	theme	non-MC-producing	629:644	arg1	microcystin-LR					560:573	pure microcystin-LR	555:573	pure microcystin-LR (MC-LR)	555:581	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	4	14	theme	non-MC-producing	629:644	arg1	extracts					592:599	cell extracts	587:599	cell extracts of one MC-producing and one non-MC-producing Microcystis strain	587:663	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	4	14	theme	non-MC-producing	629:644	arg1	MC-LR					576:580	MC-LR	576:580	MC-LR	576:580	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	3	15	theme	toxicity	418:425	arg1	effects					392:398	the effects	388:398	the effects of cyanobacterial toxicity on plant cell wall	388:444	However, the effects of cyanobacterial toxicity on plant cell wall have not been yet thoroughly studied.					
36195035	0	16	theme	root	105:108	arg1	cells					110:114	rice root cells	100:114	rice root cells	100:114	Microcystin-LR and cyanobacterial extracts alter the distribution of cell wall matrix components in rice root cells.					
36195035	5	17	theme	roots	797:801	arg1	sections					749:756	Semi-thin transverse sections	728:756	Semi-thin transverse sections of variously treated LR-White-embedded roots	728:801	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	4	18	theme	MC-producing	608:619	arg1	microcystin-LR					560:573	pure microcystin-LR	555:573	pure microcystin-LR (MC-LR)	555:581	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	4	18	theme	MC-producing	608:619	arg1	extracts					592:599	cell extracts	587:599	cell extracts of one MC-producing and one non-MC-producing Microcystis strain	587:663	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	4	18	theme	MC-producing	608:619	arg1	MC-LR					576:580	MC-LR	576:580	MC-LR	576:580	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	5	19	theme	LR-White-embedded	779:795	arg1	roots					797:801	variously treated LR-White-embedded roots	761:801	variously treated LR-White-embedded roots	761:801	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	5	20	theme	transverse	738:747	arg1	sections					749:756	Semi-thin transverse sections	728:756	Semi-thin transverse sections of variously treated LR-White-embedded roots	728:801	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	6	21	theme	distribution	978:989	arg1	patterns					991:998	arabinan distribution patterns	969:998	arabinan distribution patterns	969:998	Homogalacturonan and arabinan distribution patterns were altered in the affected roots, while a pectin methylesterase (PME) activity assay revealed that PMEs were also affected.					
36195035	1	22	theme	plant	174:178	arg1	cytoskeleton					180:191	the plant cytoskeleton	170:191	the plant cytoskeleton (i.e. microtubules and F-actin)	170:223	Cyanobacterial toxins (known as cyanotoxins) disrupt the plant cytoskeleton (i.e. microtubules and F-actin), which is implicated in the regulation of cell wall architecture.					
36195035	0	23	theme	Microcystin-LR	0:13	arg1	extracts					34:41	Microcystin-LR and cyanobacterial extracts	0:41	Microcystin-LR and cyanobacterial extracts	0:41	Microcystin-LR and cyanobacterial extracts alter the distribution of cell wall matrix components in rice root cells.					
36195035	4	24	theme	matrix	526:531	arg1	alterations					501:511	the alterations	497:511	the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain	497:663	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	9	25	theme	toxicity	1378:1385	arg1	mechanism					1350:1358	The exact mechanism	1340:1358	The exact mechanism of cyanobacterial toxicity against the cell wall	1340:1407	The exact mechanism of cyanobacterial toxicity against the cell wall is to be further investigated.					
36195035	3	26	theme	cyanobacterial	403:416	arg1	toxicity					418:425	cyanobacterial toxicity	403:425	cyanobacterial toxicity	403:425	However, the effects of cyanobacterial toxicity on plant cell wall have not been yet thoroughly studied.					
36195035	4	27	theme	wall	521:524	arg1	matrix					526:531	cell wall matrix	516:531	cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain	516:663	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	4	28	theme	root	716:719	arg1	cells					721:725	differentiated Oryza sativa (rice) root cells	681:725	differentiated Oryza sativa (rice) root cells	681:725	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	6	29	theme	methylesterase	1051:1064	arg1	assay					1081:1085	a pectin methylesterase (PME) activity assay	1042:1085	a pectin methylesterase (PME) activity assay	1042:1085	Homogalacturonan and arabinan distribution patterns were altered in the affected roots, while a pectin methylesterase (PME) activity assay revealed that PMEs were also affected.					
36195035	0	30	theme	cyanobacterial	19:32	arg1	extracts					34:41	Microcystin-LR and cyanobacterial extracts	0:41	Microcystin-LR and cyanobacterial extracts	0:41	Microcystin-LR and cyanobacterial extracts alter the distribution of cell wall matrix components in rice root cells.					
36195035	1	31	theme	cell	267:270	arg1	architecture					277:288	cell wall architecture	267:288	cell wall architecture	267:288	Cyanobacterial toxins (known as cyanotoxins) disrupt the plant cytoskeleton (i.e. microtubules and F-actin), which is implicated in the regulation of cell wall architecture.					
36195035	4	32	theme	cell	587:590	arg1	extracts					592:599	cell extracts	587:599	cell extracts of one MC-producing and one non-MC-producing Microcystis strain	587:663	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	7	33	theme	Ca2+	1149:1152	arg1	levels					1154:1159	Elevated intracellular Ca2+ levels	1126:1159	Elevated intracellular Ca2+ levels	1126:1159	Elevated intracellular Ca2+ levels, along with increased callose and mixed linkage glucans (MLGs) deposition, were also observed after treatment.					
36195035	1	34	theme	wall	272:275	arg1	architecture					277:288	cell wall architecture	267:288	cell wall architecture	267:288	Cyanobacterial toxins (known as cyanotoxins) disrupt the plant cytoskeleton (i.e. microtubules and F-actin), which is implicated in the regulation of cell wall architecture.					
36195035	5	35	theme	cell	840:843	arg1	hemicelluloses					932:945	hemicelluloses	932:945	hemicelluloses	932:945	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	5	35	theme	cell	840:843	arg1	arabinogalactan-proteins					895:918	arabinogalactan-proteins	895:918	arabinogalactan-proteins (AGPs)	895:925	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	5	35	theme	cell	840:843	arg1	epitopes					850:857	various cell wall epitopes	832:857	various cell wall epitopes	832:857	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	5	35	theme	cell	840:843	arg1	homogalacturonans					870:886	homogalacturonans	870:886	homogalacturonans (HGs)	870:892	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	3	36	theme	plant	430:434	arg1	wall					441:444	plant cell wall	430:444	plant cell wall	430:444	However, the effects of cyanobacterial toxicity on plant cell wall have not been yet thoroughly studied.					
36195035	6	37	theme	activity	1072:1079	arg1	assay					1081:1085	a pectin methylesterase (PME) activity assay	1042:1085	a pectin methylesterase (PME) activity assay	1042:1085	Homogalacturonan and arabinan distribution patterns were altered in the affected roots, while a pectin methylesterase (PME) activity assay revealed that PMEs were also affected.					
36195035	1	38	theme	architecture	277:288	arg1	regulation					253:262	the regulation	249:262	the regulation of cell wall architecture	249:288	Cyanobacterial toxins (known as cyanotoxins) disrupt the plant cytoskeleton (i.e. microtubules and F-actin), which is implicated in the regulation of cell wall architecture.					
36195035	1	39	dep	cytoskeleton	180:191	arg1	F-actin					216:222	F-actin	216:222	F-actin	216:222	Cyanobacterial toxins (known as cyanotoxins) disrupt the plant cytoskeleton (i.e. microtubules and F-actin), which is implicated in the regulation of cell wall architecture.					
36195035	1	39	dep	cytoskeleton	180:191	arg1	microtubules					199:210	microtubules	199:210	microtubules	199:210	Cyanobacterial toxins (known as cyanotoxins) disrupt the plant cytoskeleton (i.e. microtubules and F-actin), which is implicated in the regulation of cell wall architecture.					
36195035	3	40	theme	cell	436:439	arg1	wall					441:444	plant cell wall	430:444	plant cell wall	430:444	However, the effects of cyanobacterial toxicity on plant cell wall have not been yet thoroughly studied.					
36195035	7	41	theme	Elevated	1126:1133	arg1	levels					1154:1159	Elevated intracellular Ca2+ levels	1126:1159	Elevated intracellular Ca2+ levels	1126:1159	Elevated intracellular Ca2+ levels, along with increased callose and mixed linkage glucans (MLGs) deposition, were also observed after treatment.					
36195035	7	42	theme	mixed	1195:1199	arg1	MLGs					1218:1221	MLGs	1218:1221	MLGs	1218:1221	Elevated intracellular Ca2+ levels, along with increased callose and mixed linkage glucans (MLGs) deposition, were also observed after treatment.					
36195035	7	42	theme	mixed	1195:1199	arg1	glucans					1209:1215	mixed linkage glucans	1195:1215	mixed linkage glucans (MLGs)	1195:1222	Elevated intracellular Ca2+ levels, along with increased callose and mixed linkage glucans (MLGs) deposition, were also observed after treatment.					
36195035	6	43	theme	arabinan	969:976	arg1	patterns					991:998	arabinan distribution patterns	969:998	arabinan distribution patterns	969:998	Homogalacturonan and arabinan distribution patterns were altered in the affected roots, while a pectin methylesterase (PME) activity assay revealed that PMEs were also affected.					
36195035	4	44	theme	differentiated	681:694	arg1	cells					721:725	differentiated Oryza sativa (rice) root cells	681:725	differentiated Oryza sativa (rice) root cells	681:725	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	5	45	theme	treated	771:777	arg1	roots					797:801	variously treated LR-White-embedded roots	761:801	variously treated LR-White-embedded roots	761:801	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	4	46	theme	cell	516:519	arg1	matrix					526:531	cell wall matrix	516:531	cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain	516:663	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	1	47	theme	Cyanobacterial	117:130	arg1	toxins					132:137	Cyanobacterial toxins	117:137	Cyanobacterial toxins (known as cyanotoxins)	117:160	Cyanobacterial toxins (known as cyanotoxins) disrupt the plant cytoskeleton (i.e. microtubules and F-actin), which is implicated in the regulation of cell wall architecture.					
36195035	7	48	theme	intracellular	1135:1147	arg1	levels					1154:1159	Elevated intracellular Ca2+ levels	1126:1159	Elevated intracellular Ca2+ levels	1126:1159	Elevated intracellular Ca2+ levels, along with increased callose and mixed linkage glucans (MLGs) deposition, were also observed after treatment.					
36195035	1	49	dep	microtubules	199:210	arg1	i.e.					194:197	i.e.	194:197	i.e.	194:197	Cyanobacterial toxins (known as cyanotoxins) disrupt the plant cytoskeleton (i.e. microtubules and F-actin), which is implicated in the regulation of cell wall architecture.					
36195035	4	50	theme	Microcystis	646:656	arg1	strain					658:663	Microcystis strain	646:663	Microcystis strain	646:663	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	4	51	theme	sativa	702:707	arg1	cells					721:725	differentiated Oryza sativa (rice) root cells	681:725	differentiated Oryza sativa (rice) root cells	681:725	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	7	52	theme	glucans	1209:1215	arg1	deposition					1224:1233	increased callose and mixed linkage glucans (MLGs) deposition	1173:1233	increased callose and mixed linkage glucans (MLGs) deposition	1173:1233	Elevated intracellular Ca2+ levels, along with increased callose and mixed linkage glucans (MLGs) deposition, were also observed after treatment.					
36195035	4	53	with	treatments	539:548	arg1	microcystin-LR					560:573	pure microcystin-LR	555:573	pure microcystin-LR (MC-LR)	555:581	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	4	53	with	treatments	539:548	arg1	extracts					592:599	cell extracts	587:599	cell extracts of one MC-producing and one non-MC-producing Microcystis strain	587:663	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	4	53	with	treatments	539:548	arg1	MC-LR					576:580	MC-LR	576:580	MC-LR	576:580	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	4	54	theme	Oryza	696:700	arg1	rice					710:713	rice	710:713	rice	710:713	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	4	54	theme	Oryza	696:700	arg1	sativa					702:707	Oryza sativa	696:707	differentiated Oryza sativa (rice) root cells	681:725	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	9	55	theme	cell	1399:1402	arg1	wall					1404:1407	the cell wall	1395:1407	the cell wall	1395:1407	The exact mechanism of cyanobacterial toxicity against the cell wall is to be further investigated.					
36195035	0	56	theme	wall	74:77	arg1	components					86:95	cell wall matrix components	69:95	cell wall matrix components	69:95	Microcystin-LR and cyanobacterial extracts alter the distribution of cell wall matrix components in rice root cells.					
36195035	5	57	theme	wall	845:848	arg1	hemicelluloses					932:945	hemicelluloses	932:945	hemicelluloses	932:945	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	5	57	theme	wall	845:848	arg1	arabinogalactan-proteins					895:918	arabinogalactan-proteins	895:918	arabinogalactan-proteins (AGPs)	895:925	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	5	57	theme	wall	845:848	arg1	epitopes					850:857	various cell wall epitopes	832:857	various cell wall epitopes	832:857	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	5	57	theme	wall	845:848	arg1	homogalacturonans					870:886	homogalacturonans	870:886	homogalacturonans (HGs)	870:892	Semi-thin transverse sections of variously treated LR-White-embedded roots underwent immunostaining for various cell wall epitopes, including homogalacturonans (HGs), arabinogalactan-proteins (AGPs), and hemicelluloses.					
36195035	2	58	theme	wall	347:350	arg1	structure					352:360	cell wall structure	342:360	cell wall structure	342:360	Therefore, cyanotoxins are also expected to affect cell wall structure and composition.					
36195035	6	59	theme	affected	1020:1027	arg1	roots					1029:1033	the affected roots	1016:1033	the affected roots	1016:1033	Homogalacturonan and arabinan distribution patterns were altered in the affected roots, while a pectin methylesterase (PME) activity assay revealed that PMEs were also affected.					
36195035	0	60	theme	cell	69:72	arg1	components					86:95	cell wall matrix components	69:95	cell wall matrix components	69:95	Microcystin-LR and cyanobacterial extracts alter the distribution of cell wall matrix components in rice root cells.					
36195035	4	61	dep	MC-producing	608:619	arg1	strain					658:663	Microcystis strain	646:663	Microcystis strain	646:663	Accordingly, the alterations of cell wall matrix after treatments with pure microcystin-LR (MC-LR), or cell extracts of one MC-producing and one non-MC-producing Microcystis strain were studied in differentiated Oryza sativa (rice) root cells.					
36195035	2	62	theme	cell	342:345	arg1	structure					352:360	cell wall structure	342:360	cell wall structure	342:360	Therefore, cyanotoxins are also expected to affect cell wall structure and composition.					
36195035	7	63	theme	increased	1173:1181	arg1	deposition					1224:1233	increased callose and mixed linkage glucans (MLGs) deposition	1173:1233	increased callose and mixed linkage glucans (MLGs) deposition	1173:1233	Elevated intracellular Ca2+ levels, along with increased callose and mixed linkage glucans (MLGs) deposition, were also observed after treatment.					
36195035	9	64	theme	cyanobacterial	1363:1376	arg1	toxicity					1378:1385	cyanobacterial toxicity	1363:1385	cyanobacterial toxicity	1363:1385	The exact mechanism of cyanobacterial toxicity against the cell wall is to be further investigated.					
37375300	0	0	theme	Method	117:122	arg1	Development					39:49	Design-Compliant Development	22:49	Design-Compliant Development of a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities	22:177	Analytical Quality by Design-Compliant Development of a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities.					
37375300	7	1	theme	sodium	1481:1486	arg1	sulfate					1496:1502	65.0 mM sodium dodecyl sulfate	1473:1502	65.0 mM sodium dodecyl sulfate	1473:1502	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	0	2	theme	Chromatography	102:115	arg1	Method					117:122	a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method	54:122	a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities	54:177	Analytical Quality by Design-Compliant Development of a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities.					
37375300	3	3	theme	Target	711:716	arg1	Profile					718:724	the Analytical Target Profile	696:724	the Analytical Target Profile	696:724	According to the Analytical Target Profile, the procedure should be able to simultaneously quantify trimecaine and its four impurities, with specified analytical performances.					
37375300	4	4	theme	ElectroKinetic	900:913	arg1	Chromatography					915:928	Micellar ElectroKinetic Chromatography	891:928	Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin	891:1012	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	4	4	theme	ElectroKinetic	900:913	arg1	mode					882:885	The selected operative mode	859:885	The selected operative mode	859:885	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	2	5	theme	Electrophoresis	541:555	arg1	development					564:574	Capillary Electrophoresis method development	531:574	Capillary Electrophoresis method development	531:574	In the present study, these recommendations were implemented in Capillary Electrophoresis method development for the quality control of a drug product containing trimecaine, by applying Analytical Quality by Design.					
37375300	7	6	theme	mM	1478:1479	arg1	sulfate					1496:1502	65.0 mM sodium dodecyl sulfate	1473:1502	65.0 mM sodium dodecyl sulfate	1473:1502	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	2	7	theme	product	610:616	arg1	control					592:598	the quality control	580:598	the quality control of a drug product containing trimecaine	580:638	In the present study, these recommendations were implemented in Capillary Electrophoresis method development for the quality control of a drug product containing trimecaine, by applying Analytical Quality by Design.					
37375300	1	8	theme	medicinal	447:455	arg1	products					457:464	medicinal products	447:464	medicinal products	447:464	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	6	9	theme	resolution	1289:1298	arg1	values					1300:1305	critical resolution values	1280:1305	critical resolution values	1280:1305	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	6	9	theme	resolution	1289:1298	arg1	Attributes					1219:1228	The Critical Method Attributes	1199:1228	The Critical Method Attributes	1199:1228	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	7	10	theme	%	1514:1514	arg1	n-butanol					1520:1528	0.25-1.29% v/v n-butanol	1505:1528	0.25-1.29% v/v n-butanol	1505:1528	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	2	11	theme	Capillary	531:539	arg1	Electrophoresis					541:555	Capillary Electrophoresis	531:555	Capillary Electrophoresis method development	531:574	In the present study, these recommendations were implemented in Capillary Electrophoresis method development for the quality control of a drug product containing trimecaine, by applying Analytical Quality by Design.					
37375300	2	12	theme	drug	605:608	arg1	product					610:616	a drug product	603:616	a drug product containing trimecaine	603:638	In the present study, these recommendations were implemented in Capillary Electrophoresis method development for the quality control of a drug product containing trimecaine, by applying Analytical Quality by Design.					
37375300	1	13	theme	products	457:464	arg1	quality					436:442	the quality	432:442	the quality of medicinal products	432:464	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	5	14	theme	Knowledge	1049:1057	arg1	Space					1059:1063	The Knowledge Space	1045:1063	The Knowledge Space	1045:1063	The Knowledge Space was investigated through a screening matrix encompassing the composition of the background electrolyte and the instrumental settings.					
37375300	7	15	theme	v/v	1516:1518	arg1	n-butanol					1520:1528	0.25-1.29% v/v n-butanol	1505:1528	0.25-1.29% v/v n-butanol	1505:1528	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	16	theme	Method	1395:1400	arg1	Region					1418:1423	the Method Operable Design Region	1391:1423	the Method Operable Design Region	1391:1423	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	2	17	theme	Analytical	653:662	arg1	Quality					664:670	Analytical Quality	653:670	Analytical Quality	653:670	In the present study, these recommendations were implemented in Capillary Electrophoresis method development for the quality control of a drug product containing trimecaine, by applying Analytical Quality by Design.					
37375300	3	18	with	impurities	807:816	arg1	performances					845:856	specified analytical performances	824:856	specified analytical performances	824:856	According to the Analytical Target Profile, the procedure should be able to simultaneously quantify trimecaine and its four impurities, with specified analytical performances.					
37375300	7	19	theme	buffer	1452:1457	arg1	kV					1600:1601	23-29 kV	1594:1601	23-29 kV	1594:1601	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	19	theme	buffer	1452:1457	arg1	pH					1459:1460	21-26 mM phosphate-borate buffer pH 9.50-9.77	1426:1470	21-26 mM phosphate-borate buffer pH 9.50-9.77	1426:1470	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	4	20	theme	operative	872:880	arg1	Chromatography					915:928	Micellar ElectroKinetic Chromatography	891:928	Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin	891:1012	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	4	20	theme	operative	872:880	arg1	mode					882:885	The selected operative mode	859:885	The selected operative mode	859:885	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	7	21	theme	21-26	1531:1535	arg1	mM					1537:1538	mM	1537:1538	mM	1537:1538	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	5	22	theme	instrumental	1176:1187	arg1	settings					1189:1196	the instrumental settings	1172:1196	the instrumental settings	1172:1196	The Knowledge Space was investigated through a screening matrix encompassing the composition of the background electrolyte and the instrumental settings.					
37375300	8	23	theme	ampoules	1644:1651	arg1	products					1658:1665	ampoules drug products	1644:1665	ampoules drug products	1644:1665	The method was validated and applied to ampoules drug products.					
37375300	4	24	theme	Micellar	891:898	arg1	Chromatography					915:928	Micellar ElectroKinetic Chromatography	891:928	Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin	891:1012	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	4	24	theme	Micellar	891:898	arg1	mode					882:885	The selected operative mode	859:885	The selected operative mode	859:885	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	7	25	theme	Monte	1341:1345	arg1	Simulations					1353:1363	Monte Carlo Simulations	1341:1363	Monte Carlo Simulations	1341:1363	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	4	26	theme	phosphate-borate	1020:1035	arg1	buffer					1037:1042	a phosphate-borate buffer	1018:1042	a phosphate-borate buffer	1018:1042	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	7	27	theme	mM	1432:1433	arg1	kV					1600:1601	23-29 kV	1594:1601	23-29 kV	1594:1601	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	27	theme	mM	1432:1433	arg1	pH					1459:1460	21-26 mM phosphate-borate buffer pH 9.50-9.77	1426:1470	21-26 mM phosphate-borate buffer pH 9.50-9.77	1426:1470	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	0	28	theme	Analytical	0:9	arg1	Quality					11:17	Analytical Quality	0:17	Analytical Quality by Design-Compliant Development of a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities.	0:178	Analytical Quality by Design-Compliant Development of a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities.					
37375300	7	29	theme	mM	1537:1538	arg1	dimethyl-β-cyclodextrin					1540:1562	21-26 mM dimethyl-β-cyclodextrin	1531:1562	21-26 mM dimethyl-β-cyclodextrin	1531:1562	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	30	dep	pH	1459:1460	arg1	n-butanol					1520:1528	0.25-1.29% v/v n-butanol	1505:1528	0.25-1.29% v/v n-butanol	1505:1528	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	30	dep	pH	1459:1460	arg1	temperature					1565:1575	temperature	1565:1575	temperature	1565:1575	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	30	dep	pH	1459:1460	arg1	sulfate					1496:1502	65.0 mM sodium dodecyl sulfate	1473:1502	65.0 mM sodium dodecyl sulfate	1473:1502	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	30	dep	pH	1459:1460	arg1	°C					1581:1582	22 °C	1578:1582	22 °C	1578:1582	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	30	dep	pH	1459:1460	arg1	dimethyl-β-cyclodextrin					1540:1562	21-26 mM dimethyl-β-cyclodextrin	1531:1562	21-26 mM dimethyl-β-cyclodextrin	1531:1562	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	30	dep	pH	1459:1460	arg1	voltage					1585:1591	voltage	1585:1591	voltage	1585:1591	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	31	theme	Design	1411:1416	arg1	Region					1418:1423	the Method Operable Design Region	1391:1423	the Method Operable Design Region	1391:1423	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	32	theme	phosphate-borate	1435:1450	arg1	kV					1600:1601	23-29 kV	1594:1601	23-29 kV	1594:1601	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	32	theme	phosphate-borate	1435:1450	arg1	pH					1459:1460	21-26 mM phosphate-borate buffer pH 9.50-9.77	1426:1470	21-26 mM phosphate-borate buffer pH 9.50-9.77	1426:1470	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	0	33	theme	Design-Compliant	22:37	arg1	Development					39:49	Design-Compliant Development	22:49	Design-Compliant Development of a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities	22:177	Analytical Quality by Design-Compliant Development of a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities.					
37375300	3	34	with	trimecaine	783:792	arg1	performances					845:856	specified analytical performances	824:856	specified analytical performances	824:856	According to the Analytical Target Profile, the procedure should be able to simultaneously quantify trimecaine and its four impurities, with specified analytical performances.					
37375300	7	35	theme	0.25-1.29	1505:1513	arg1	%					1514:1514	%	1514:1514	%	1514:1514	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	8	36	theme	drug	1653:1656	arg1	products					1658:1665	ampoules drug products	1644:1665	ampoules drug products	1644:1665	The method was validated and applied to ampoules drug products.					
37375300	0	37	theme	Trimecaine	149:158	arg1	Determination					132:144	the Determination	128:144	the Determination of Trimecaine and Its Impurities	128:177	Analytical Quality by Design-Compliant Development of a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities.					
37375300	6	38	theme	analysis	1249:1256	arg1	time					1258:1261	analysis time	1249:1261	analysis time	1249:1261	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	6	38	theme	analysis	1249:1256	arg1	Attributes					1219:1228	The Critical Method Attributes	1199:1228	The Critical Method Attributes	1199:1228	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	7	39	theme	21-26	1426:1430	arg1	mM					1432:1433	mM	1432:1433	mM	1432:1433	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	40	theme	Response	1308:1315	arg1	Methodology					1325:1335	Response Surface Methodology	1308:1335	Response Surface Methodology	1308:1335	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	1	41	theme	development	300:310	arg1	activities					327:336	the development and validation activities	296:336	the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products	296:464	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	0	42	theme	Impurities	168:177	arg1	Determination					132:144	the Determination	128:144	the Determination of Trimecaine and Its Impurities	128:177	Analytical Quality by Design-Compliant Development of a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities.					
37375300	1	43	theme	analytical	391:400	arg1	technique					402:410	an analytical technique	388:410	an analytical technique addressed to assess the quality of medicinal products	388:464	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	1	44	theme	draft	242:246	arg1	Q14					270:272	Q14	270:272	Q14	270:272	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	1	44	theme	draft	242:246	arg1	Q2					259:260	Q2	259:260	Q2(R2)	259:264	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	1	44	theme	draft	242:246	arg1	guidelines					248:257	draft guidelines	242:257	draft guidelines Q2(R2) and Q14	242:272	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	2	45	theme	present	474:480	arg1	study					482:486	the present study	470:486	the present study	470:486	In the present study, these recommendations were implemented in Capillary Electrophoresis method development for the quality control of a drug product containing trimecaine, by applying Analytical Quality by Design.					
37375300	2	46	theme	method	557:562	arg1	development					564:574	Capillary Electrophoresis method development	531:574	Capillary Electrophoresis method development	531:574	In the present study, these recommendations were implemented in Capillary Electrophoresis method development for the quality control of a drug product containing trimecaine, by applying Analytical Quality by Design.					
37375300	1	47	theme	technique	402:410	arg1	lifespan					376:383	the lifespan	372:383	the lifespan of an analytical technique addressed to assess the quality of medicinal products	372:464	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	7	48	theme	Region	1418:1423	arg1	definition					1377:1386	the definition	1373:1386	the definition of the Method Operable Design Region	1373:1423	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	0	49	theme	Cyclodextrin-Modified	56:76	arg1	Method					117:122	a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method	54:122	a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities	54:177	Analytical Quality by Design-Compliant Development of a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities.					
37375300	1	50	theme	validation	316:325	arg1	activities					327:336	the development and validation activities	296:336	the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products	296:464	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	4	51	theme	sulfate	955:961	arg1	micelles					963:970	sodium dodecyl sulfate micelles	940:970	sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin	940:1012	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	5	52	theme	settings	1189:1196	arg1	composition					1126:1136	the composition	1122:1136	the composition of the background electrolyte and the instrumental settings	1122:1196	The Knowledge Space was investigated through a screening matrix encompassing the composition of the background electrolyte and the instrumental settings.					
37375300	4	53	theme	selected	863:870	arg1	Chromatography					915:928	Micellar ElectroKinetic Chromatography	891:928	Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin	891:1012	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	4	53	theme	selected	863:870	arg1	mode					882:885	The selected operative mode	859:885	The selected operative mode	859:885	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	4	54	from	Chromatography	915:928	arg1	buffer					1037:1042	a phosphate-borate buffer	1018:1042	a phosphate-borate buffer	1018:1042	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	6	55	theme	critical	1280:1287	arg1	values					1300:1305	critical resolution values	1280:1305	critical resolution values	1280:1305	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	6	55	theme	critical	1280:1287	arg1	Attributes					1219:1228	The Critical Method Attributes	1199:1228	The Critical Method Attributes	1199:1228	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	4	56	theme	dodecyl	947:953	arg1	micelles					963:970	sodium dodecyl sulfate micelles	940:970	sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin	940:1012	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	7	57	theme	dodecyl	1488:1494	arg1	sulfate					1496:1502	65.0 mM sodium dodecyl sulfate	1473:1502	65.0 mM sodium dodecyl sulfate	1473:1502	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	4	58	theme	sodium	940:945	arg1	micelles					963:970	sodium dodecyl sulfate micelles	940:970	sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin	940:1012	The selected operative mode was Micellar ElectroKinetic Chromatography employing sodium dodecyl sulfate micelles supplemented with dimethyl-β-cyclodextrin, in a phosphate-borate buffer.					
37375300	3	59	theme	Analytical	700:709	arg1	Profile					718:724	the Analytical Target Profile	696:724	the Analytical Target Profile	696:724	According to the Analytical Target Profile, the procedure should be able to simultaneously quantify trimecaine and its four impurities, with specified analytical performances.					
37375300	7	60	theme	65.0	1473:1476	arg1	mM					1478:1479	mM	1478:1479	mM	1478:1479	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	3	61	theme	specified	824:832	arg1	performances					845:856	specified analytical performances	824:856	specified analytical performances	824:856	According to the Analytical Target Profile, the procedure should be able to simultaneously quantify trimecaine and its four impurities, with specified analytical performances.					
37375300	2	62	theme	quality	584:590	arg1	control					592:598	the quality control	580:598	the quality control of a drug product containing trimecaine	580:638	In the present study, these recommendations were implemented in Capillary Electrophoresis method development for the quality control of a drug product containing trimecaine, by applying Analytical Quality by Design.					
37375300	5	63	theme	background	1145:1154	arg1	electrolyte					1156:1166	the background electrolyte	1141:1166	the background electrolyte	1141:1166	The Knowledge Space was investigated through a screening matrix encompassing the composition of the background electrolyte and the instrumental settings.					
37375300	1	64	dep	guidelines	248:257	arg1	R2					262:263	R2	262:263	R2	262:263	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	1	64	dep	guidelines	248:257	arg1	Q14					270:272	Q14	270:272	Q14	270:272	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	1	64	dep	guidelines	248:257	arg1	Q2					259:260	Q2	259:260	Q2(R2)	259:264	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	1	64	dep	guidelines	248:257	arg1	guidelines					248:257	draft guidelines	242:257	draft guidelines Q2(R2) and Q14	242:272	In 2022, the International Council for Harmonisation released draft guidelines Q2(R2) and Q14, intending to specify the development and validation activities that should be carried out during the lifespan of an analytical technique addressed to assess the quality of medicinal products.					
37375300	2	65	contain	containing	618:627	arg2	trimecaine					629:638	trimecaine	629:638	trimecaine	629:638	In the present study, these recommendations were implemented in Capillary Electrophoresis method development for the quality control of a drug product containing trimecaine, by applying Analytical Quality by Design.					
37375300	2	65	contain	containing	618:627	arg1	product					610:616	a drug product	603:616	a drug product containing trimecaine	603:638	In the present study, these recommendations were implemented in Capillary Electrophoresis method development for the quality control of a drug product containing trimecaine, by applying Analytical Quality by Design.					
37375300	3	66	theme	analytical	834:843	arg1	performances					845:856	specified analytical performances	824:856	specified analytical performances	824:856	According to the Analytical Target Profile, the procedure should be able to simultaneously quantify trimecaine and its four impurities, with specified analytical performances.					
37375300	0	67	theme	ElectroKinetic	87:100	arg1	Method					117:122	a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method	54:122	a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities	54:177	Analytical Quality by Design-Compliant Development of a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities.					
37375300	5	68	theme	electrolyte	1156:1166	arg1	composition					1126:1136	the composition	1122:1136	the composition of the background electrolyte and the instrumental settings	1122:1196	The Knowledge Space was investigated through a screening matrix encompassing the composition of the background electrolyte and the instrumental settings.					
37375300	6	69	theme	Method	1212:1217	arg1	values					1300:1305	critical resolution values	1280:1305	critical resolution values	1280:1305	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	6	69	theme	Method	1212:1217	arg1	efficiency					1264:1273	efficiency	1264:1273	efficiency	1264:1273	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	6	69	theme	Method	1212:1217	arg1	time					1258:1261	analysis time	1249:1261	analysis time	1249:1261	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	6	69	theme	Method	1212:1217	arg1	Attributes					1219:1228	The Critical Method Attributes	1199:1228	The Critical Method Attributes	1199:1228	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	7	70	theme	Carlo	1347:1351	arg1	Simulations					1353:1363	Monte Carlo Simulations	1341:1363	Monte Carlo Simulations	1341:1363	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	0	71	theme	Micellar	78:85	arg1	Method					117:122	a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method	54:122	a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities	54:177	Analytical Quality by Design-Compliant Development of a Cyclodextrin-Modified Micellar ElectroKinetic Chromatography Method for the Determination of Trimecaine and Its Impurities.					
37375300	5	72	theme	screening	1092:1100	arg1	matrix					1102:1107	a screening matrix	1090:1107	a screening matrix encompassing the composition of the background electrolyte and the instrumental settings	1090:1196	The Knowledge Space was investigated through a screening matrix encompassing the composition of the background electrolyte and the instrumental settings.					
37375300	6	73	theme	Critical	1203:1210	arg1	values					1300:1305	critical resolution values	1280:1305	critical resolution values	1280:1305	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	6	73	theme	Critical	1203:1210	arg1	efficiency					1264:1273	efficiency	1264:1273	efficiency	1264:1273	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	6	73	theme	Critical	1203:1210	arg1	time					1258:1261	analysis time	1249:1261	analysis time	1249:1261	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	6	73	theme	Critical	1203:1210	arg1	Attributes					1219:1228	The Critical Method Attributes	1199:1228	The Critical Method Attributes	1199:1228	The Critical Method Attributes were identified as analysis time, efficiency, and critical resolution values.					
37375300	7	74	theme	Operable	1402:1409	arg1	Region					1418:1423	the Method Operable Design Region	1391:1423	the Method Operable Design Region	1391:1423	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
37375300	7	75	theme	Surface	1317:1323	arg1	Methodology					1325:1335	Response Surface Methodology	1308:1335	Response Surface Methodology	1308:1335	Response Surface Methodology and Monte Carlo Simulations allowed the definition of the Method Operable Design Region: 21-26 mM phosphate-borate buffer pH 9.50-9.77; 65.0 mM sodium dodecyl sulfate; 0.25-1.29% v/v n-butanol; 21-26 mM dimethyl-β-cyclodextrin; temperature, 22 °C; voltage, 23-29 kV.					
36713378	7	0	theme	integrity	1778:1786	arg1	restoration					1760:1770	the restoration	1756:1770	the restoration of GB integrity and immune homeostasis	1756:1809	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	2	1	theme	cells	535:539	arg1	activation					504:513	activation	504:513	activation of islet-reactive T cells	504:539	In support to this idea, we recently demonstrated that breakage of GB integrity leads to activation of islet-reactive T cells and triggers autoimmune Type 1 Diabetes (T1D).					
36713378	7	2	theme	species	2070:2076	arg1	abundance					2030:2038	increased relative abundance	2011:2038	increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila	2011:2137	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	1	3	from	gut	380:382	arg1	islets					407:412	the pancreatic islets	392:412	the pancreatic islets	392:412	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	1	3	from	gut	380:382	arg1	organs					357:362	organs	357:362	organs distal from the gut such as the pancreatic islets	357:412	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	8	4	dep	tolerogenic	2276:2286	arg1	AID					2288:2290	AID	2288:2290	AID	2288:2290	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	5	5	theme	structural	1265:1274	arg1	mucins					1304:1309	structural (Muc2) and immunoregulatory mucins	1265:1309	structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3)	1265:1325	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	5	5	theme	structural	1265:1274	arg1	Muc3					1321:1324	Muc3	1321:1324	Muc3	1321:1324	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	5	5	theme	structural	1265:1274	arg1	Muc1					1312:1315	Muc1	1312:1315	Muc1	1312:1315	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	2	6	theme	islet-reactive	518:531	arg1	cells					535:539	islet-reactive T cells	518:539	islet-reactive T cells	518:539	In support to this idea, we recently demonstrated that breakage of GB integrity leads to activation of islet-reactive T cells and triggers autoimmune Type 1 Diabetes (T1D).					
36713378	5	7	dep	the	1137:1139	arg1	AID					1141:1143	AID	1141:1143	AID	1141:1143	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	2	8	theme	Type	565:568	arg1	T1D					582:584	T1D	582:584	T1D	582:584	In support to this idea, we recently demonstrated that breakage of GB integrity leads to activation of islet-reactive T cells and triggers autoimmune Type 1 Diabetes (T1D).					
36713378	2	8	theme	Type	565:568	arg1	Diabetes					572:579	autoimmune Type 1 Diabetes	554:579	autoimmune Type 1 Diabetes (T1D)	554:585	In support to this idea, we recently demonstrated that breakage of GB integrity leads to activation of islet-reactive T cells and triggers autoimmune Type 1 Diabetes (T1D).					
36713378	3	9	theme	NOD	614:616	arg1	mice					618:621	the NOD mice	610:621	the NOD mice	610:621	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	3	9	theme	NOD	614:616	arg1	model					640:644	the spontaneous model	624:644	the spontaneous model of autoimmune diabetes	624:667	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	1	10	theme	pancreatic	396:405	arg1	islets					407:412	the pancreatic islets	392:412	the pancreatic islets	392:412	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	5	11	dep	Results	1115:1121	arg1	found					1126:1130	found	1126:1130	found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1)	1126:1374	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	4	12	theme	anti-inflammatory	992:1008	arg1	diet					1010:1013	an anti-inflammatory diet	989:1013	an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA)	989:1070	Methods Here we restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA) and tested the effect on T1D pathogenesis.					
36713378	6	13	theme	pancreatic	1659:1668	arg1	PLN					1683:1685	PLN	1683:1685	PLN	1683:1685	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	13	theme	pancreatic	1659:1668	arg1	nodes					1676:1680	pancreatic lymph nodes	1659:1680	pancreatic lymph nodes (PLN)	1659:1686	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	14	from	expenses	1605:1612	arg1	reduction					1419:1427	reduction	1419:1427	reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice	1419:1740	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	14	from	expenses	1605:1612	arg1	intestine					1648:1656	the intestine	1644:1656	the intestine	1644:1656	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	2	15	theme	GB	482:483	arg1	integrity					485:493	GB integrity	482:493	GB integrity	482:493	In support to this idea, we recently demonstrated that breakage of GB integrity leads to activation of islet-reactive T cells and triggers autoimmune Type 1 Diabetes (T1D).					
36713378	3	16	theme	T1D	897:899	arg1	occurrence					872:881	occurrence	872:881	occurrence of autoimmune T1D	872:899	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	3	16	theme	T1D	897:899	arg1	breakage					839:846	breakage	839:846	breakage of the GB integrity	839:866	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	4	17	theme	NOD	954:956	arg1	mice					958:961	the NOD mice	950:961	the NOD mice	950:961	Methods Here we restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA) and tested the effect on T1D pathogenesis.					
36713378	7	18	theme	increased	2011:2019	arg1	abundance					2030:2038	increased relative abundance	2011:2038	increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila	2011:2137	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	3	19	theme	spontaneous	628:638	arg1	mice					618:621	the NOD mice	610:621	the NOD mice	610:621	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	3	19	theme	spontaneous	628:638	arg1	model					640:644	the spontaneous model	624:644	the spontaneous model of autoimmune diabetes	624:667	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	1	20	theme	gut	173:175	arg1	GB					186:187	GB	186:187	GB	186:187	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	1	20	theme	gut	173:175	arg1	barrier					177:183	the gut barrier	169:183	the gut barrier (GB)	169:188	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	5	21	dep	mucins	1304:1309	arg1	mucins					1304:1309	structural (Muc2) and immunoregulatory mucins	1265:1309	structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3)	1265:1325	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	5	21	dep	mucins	1304:1309	arg1	Muc3					1321:1324	Muc3	1321:1324	Muc3	1321:1324	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	5	21	dep	mucins	1304:1309	arg1	Muc1					1312:1315	Muc1	1312:1315	Muc1	1312:1315	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	6	22	dep	expression	1471:1480	arg1	i.e.					1457:1460	i.e.	1457:1460	i.e.	1457:1460	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	1	23	dep	fundamental	193:203	arg1	integrity					156:164	The integrity	152:164	The integrity of the gut barrier (GB)	152:188	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	1	23	dep	fundamental	193:203	arg1	regulate					208:215	regulate	208:215	to regulate the crosstalk between the microbiota and the immune system	205:274	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	1	23	dep	fundamental	193:203	arg1	fundamental					193:203	fundamental	193:203	fundamental	193:203	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	1	23	dep	fundamental	193:203	arg1	prevent					283:289	prevent	283:289	to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets	280:412	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	0	24	theme	immune	107:112	arg1	homeostasis					114:124	immune homeostasis	107:124	immune homeostasis	107:124	A diet enriched in omega-3 PUFA and inulin prevents type 1 diabetes by restoring gut barrier integrity and immune homeostasis in NOD mice.					
36713378	8	25	theme	integrity	2204:2212	arg1	restoration					2186:2196	the restoration	2182:2196	the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles	2182:2344	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	6	26	theme	IL-1β	1485:1489	arg1	expression					1471:1480	reduced expression	1463:1480	reduced expression of IL-1β	1463:1489	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	26	theme	IL-1β	1485:1489	arg1	IL-23					1492:1496	IL-23	1492:1496	IL-23	1492:1496	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	26	theme	IL-1β	1485:1489	arg1	transcripts					1508:1518	IL-17 transcripts	1502:1518	IL-17 transcripts	1502:1518	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	4	27	theme	soluble	1033:1039	arg1	inulin					1047:1052	soluble fiber inulin	1033:1052	soluble fiber inulin	1033:1052	Methods Here we restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA) and tested the effect on T1D pathogenesis.					
36713378	7	28	theme	anti-inflammatory	1859:1875	arg1	metabolites					1877:1887	anti-inflammatory metabolites	1859:1887	anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways	1859:1960	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	3	29	theme	diabetes	660:667	arg1	mice					618:621	the NOD mice	610:621	the NOD mice	610:621	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	3	29	theme	diabetes	660:667	arg1	model					640:644	the spontaneous model	624:644	the spontaneous model of autoimmune diabetes	624:667	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	6	30	theme	GB	1392:1393	arg1	integrity					1395:1403	GB integrity	1392:1403	GB integrity	1392:1403	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	8	31	theme	intestinal	2218:2227	arg1	homeostasis					2236:2246	intestinal immune homeostasis	2218:2246	intestinal immune homeostasis	2218:2246	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	4	32	dep	diet	1010:1013	arg1	AID-					1016:1019	AID-	1016:1019	AID- enriched in soluble fiber inulin and omega 3-PUFA	1016:1069	Methods Here we restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA) and tested the effect on T1D pathogenesis.					
36713378	5	33	dep	structural	1265:1274	arg1	Muc2					1277:1280	Muc2	1277:1280	Muc2	1277:1280	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	8	34	theme	homeostasis	2236:2246	arg1	restoration					2186:2196	the restoration	2182:2196	the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles	2182:2344	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	8	35	theme	gut	2309:2311	arg1	profiles					2337:2344	the gut microbial and metabolic profiles	2305:2344	the gut microbial and metabolic profiles	2305:2344	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	6	36	attach	linked	1409:1414	arg1	reduction					1419:1427	reduction	1419:1427	reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice	1419:1740	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	36	attach	linked	1409:1414	arg2	Restoration					1377:1387	Restoration	1377:1387	Restoration of GB integrity	1377:1403	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	37	theme	regulatory	1538:1547	arg1	cells					1551:1555	regulatory T cells	1538:1555	regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells)	1538:1596	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	3	38	theme	mucus	760:764	arg1	layer					766:770	the mucus layer	756:770	the mucus layer	756:770	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	6	39	theme	expansion	1525:1533	arg1	reduction					1419:1427	reduction	1419:1427	reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice	1419:1740	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	3	40	theme	T1D	591:593	arg1	patients					595:602	T1D patients	591:602	T1D patients	591:602	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	6	41	theme	inflammation	1443:1454	arg1	reduction					1419:1427	reduction	1419:1427	reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice	1419:1740	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	4	42	from	effect	1087:1092	arg1	pathogenesis					1101:1112	T1D pathogenesis	1097:1112	T1D pathogenesis	1097:1112	Methods Here we restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA) and tested the effect on T1D pathogenesis.					
36713378	7	43	theme	relative	2021:2028	arg1	abundance					2030:2038	increased relative abundance	2011:2038	increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila	2011:2137	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	8	44	theme	autoimmune	2355:2364	arg1	T1D					2366:2368	autoimmune T1D	2355:2368	autoimmune T1D in preclinical models	2355:2390	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	0	45	theme	gut	81:83	arg1	integrity					93:101	gut barrier integrity	81:101	gut barrier integrity	81:101	A diet enriched in omega-3 PUFA and inulin prevents type 1 diabetes by restoring gut barrier integrity and immune homeostasis in NOD mice.					
36713378	6	46	theme	IL-17	1502:1506	arg1	transcripts					1508:1518	IL-17 transcripts	1502:1518	IL-17 transcripts	1502:1518	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	5	47	theme	mucins	1304:1309	arg1	transcripts					1250:1260	higher mRNA transcripts	1238:1260	higher mRNA transcripts	1238:1260	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	5	47	theme	mucins	1304:1309	arg1	thickness					1224:1232	increased mucus layer thickness	1202:1232	increased mucus layer thickness	1202:1232	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	4	48	theme	omega	1058:1062	arg1	3-PUFA					1064:1069	omega 3-PUFA	1058:1069	omega 3-PUFA	1058:1069	Methods Here we restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA) and tested the effect on T1D pathogenesis.					
36713378	6	49	theme	NOD	1733:1735	arg1	mice					1737:1740	AID-fed NOD mice	1725:1740	AID-fed NOD mice	1725:1740	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	50	theme	IL-10+	1580:1585	arg1	cells					1591:1595	IL-10+ Tr1 cells	1580:1595	IL-10+ Tr1 cells	1580:1595	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	5	51	theme	tight	1341:1345	arg1	claudin1					1366:1373	claudin1	1366:1373	claudin1	1366:1373	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	5	51	theme	tight	1341:1345	arg1	proteins					1356:1363	tight junction proteins	1341:1363	tight junction proteins (claudin1)	1341:1374	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	7	52	theme	microbiome	1987:1996	arg1	profile					1998:2004	the microbiome profile	1983:2004	the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila	1983:2137	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	5	53	theme	proteins	1356:1363	arg1	transcripts					1250:1260	higher mRNA transcripts	1238:1260	higher mRNA transcripts	1238:1260	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	5	53	theme	proteins	1356:1363	arg1	thickness					1224:1232	increased mucus layer thickness	1202:1232	increased mucus layer thickness	1202:1232	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	0	54	theme	omega-3	19:25	arg1	PUFA					27:30	omega-3 PUFA	19:30	omega-3 PUFA	19:30	A diet enriched in omega-3 PUFA and inulin prevents type 1 diabetes by restoring gut barrier integrity and immune homeostasis in NOD mice.					
36713378	6	55	theme	FoxP3+	1558:1563	arg1	cells					1570:1574	FoxP3+ Treg cells	1558:1574	FoxP3+ Treg cells	1558:1574	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	1	56	dep	Introduction	139:150	arg1	integrity					156:164	The integrity	152:164	The integrity of the gut barrier (GB)	152:188	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	1	56	dep	Introduction	139:150	arg1	regulate					208:215	regulate	208:215	to regulate the crosstalk between the microbiota and the immune system	205:274	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	1	56	dep	Introduction	139:150	arg1	fundamental					193:203	fundamental	193:203	fundamental	193:203	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	1	56	dep	Introduction	139:150	arg1	prevent					283:289	prevent	283:289	to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets	280:412	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	5	57	theme	layer	1218:1222	arg1	thickness					1224:1232	increased mucus layer thickness	1202:1232	increased mucus layer thickness	1202:1232	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	6	58	theme	cells	1551:1555	arg1	inflammation					1443:1454	intestinal inflammation	1432:1454	intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts)	1432:1519	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	58	theme	cells	1551:1555	arg1	expansion					1525:1533	expansion	1525:1533	expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells)	1525:1596	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	5	59	theme	higher	1238:1243	arg1	transcripts					1250:1260	higher mRNA transcripts	1238:1260	higher mRNA transcripts	1238:1260	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	7	60	theme	arachidonic	1941:1951	arg1	pathways					1953:1960	arachidonic pathways	1941:1960	arachidonic pathways	1941:1960	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	6	61	theme	effector	1617:1624	arg1	cells					1635:1639	effector Th1/Th17 cells	1617:1639	effector Th1/Th17 cells	1617:1639	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	7	62	theme	homeostasis	1799:1809	arg1	restoration					1760:1770	the restoration	1756:1770	the restoration of GB integrity and immune homeostasis	1756:1809	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	5	63	theme	increased	1202:1210	arg1	thickness					1224:1232	increased mucus layer thickness	1202:1232	increased mucus layer thickness	1202:1232	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	2	64	theme	T	533:533	arg1	cells					535:539	islet-reactive T cells	518:539	islet-reactive T cells	518:539	In support to this idea, we recently demonstrated that breakage of GB integrity leads to activation of islet-reactive T cells and triggers autoimmune Type 1 Diabetes (T1D).					
36713378	7	65	theme	GB	1775:1776	arg1	integrity					1778:1786	GB integrity	1775:1786	GB integrity	1775:1786	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	3	66	theme	integrity	858:866	arg1	occurrence					872:881	occurrence	872:881	occurrence of autoimmune T1D	872:899	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	3	66	theme	integrity	858:866	arg1	breakage					839:846	breakage	839:846	breakage of the GB integrity	839:866	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	7	67	theme	fatty	1918:1922	arg1	acids					1924:1928	the ω3/ω6 polyunsaturated fatty acids	1892:1928	the ω3/ω6 polyunsaturated fatty acids (PUFA)	1892:1935	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	7	67	theme	fatty	1918:1922	arg1	PUFA					1931:1934	PUFA	1931:1934	PUFA	1931:1934	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	2	68	theme	autoimmune	554:563	arg1	T1D					582:584	T1D	582:584	T1D	582:584	In support to this idea, we recently demonstrated that breakage of GB integrity leads to activation of islet-reactive T cells and triggers autoimmune Type 1 Diabetes (T1D).					
36713378	2	68	theme	autoimmune	554:563	arg1	Diabetes					572:579	autoimmune Type 1 Diabetes	554:579	autoimmune Type 1 Diabetes (T1D)	554:585	In support to this idea, we recently demonstrated that breakage of GB integrity leads to activation of islet-reactive T cells and triggers autoimmune Type 1 Diabetes (T1D).					
36713378	4	69	theme	diet	1010:1013	arg1	administration					971:984	administration	971:984	administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA)	971:1070	Methods Here we restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA) and tested the effect on T1D pathogenesis.					
36713378	6	70	theme	lymph	1670:1674	arg1	PLN					1683:1685	PLN	1683:1685	PLN	1683:1685	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	70	theme	lymph	1670:1674	arg1	nodes					1676:1680	pancreatic lymph nodes	1659:1680	pancreatic lymph nodes (PLN)	1659:1686	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	5	71	theme	mRNA	1245:1248	arg1	transcripts					1250:1260	higher mRNA transcripts	1238:1260	higher mRNA transcripts	1238:1260	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	7	72	with	profile	1998:2004	arg1	abundance					2030:2038	increased relative abundance	2011:2038	increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila	2011:2137	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	3	73	theme	autoimmune	886:895	arg1	T1D					897:899	autoimmune T1D	886:899	autoimmune T1D	886:899	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	7	74	theme	acids	1924:1928	arg1	metabolites					1877:1887	anti-inflammatory metabolites	1859:1887	anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways	1859:1960	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	3	75	theme	autoimmune	649:658	arg1	diabetes					660:667	autoimmune diabetes	649:667	autoimmune diabetes	649:667	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	8	76	theme	GB	2201:2202	arg1	integrity					2204:2212	GB integrity	2201:2212	GB integrity	2201:2212	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	7	77	theme	polyunsaturated	1902:1916	arg1	acids					1924:1928	the ω3/ω6 polyunsaturated fatty acids	1892:1928	the ω3/ω6 polyunsaturated fatty acids (PUFA)	1892:1935	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	7	77	theme	polyunsaturated	1902:1916	arg1	PUFA					1931:1934	PUFA	1931:1934	PUFA	1931:1934	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	1	78	theme	barrier	177:183	arg1	integrity					156:164	The integrity	152:164	The integrity of the gut barrier (GB)	152:188	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	1	78	theme	barrier	177:183	arg1	regulate					208:215	regulate	208:215	to regulate the crosstalk between the microbiota and the immune system	205:274	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	1	78	theme	barrier	177:183	arg1	fundamental					193:203	fundamental	193:203	fundamental	193:203	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	1	78	theme	barrier	177:183	arg1	prevent					283:289	prevent	283:289	to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets	280:412	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	4	79	theme	fiber	1041:1045	arg1	inulin					1047:1052	soluble fiber inulin	1033:1052	soluble fiber inulin	1033:1052	Methods Here we restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA) and tested the effect on T1D pathogenesis.					
36713378	6	80	theme	cells	1635:1639	arg1	lymphocytes					1704:1714	intra-islet lymphocytes	1692:1714	intra-islet lymphocytes (IIL)	1692:1720	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	80	theme	cells	1635:1639	arg1	IIL					1717:1719	IIL	1717:1719	IIL	1717:1719	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	80	theme	cells	1635:1639	arg1	PLN					1683:1685	PLN	1683:1685	PLN	1683:1685	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	80	theme	cells	1635:1639	arg1	expenses					1605:1612	the expenses	1601:1612	the expenses of effector Th1/Th17 cells in the intestine	1601:1656	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	80	theme	cells	1635:1639	arg1	nodes					1676:1680	pancreatic lymph nodes	1659:1680	pancreatic lymph nodes (PLN)	1659:1686	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	0	81	theme	NOD	129:131	arg1	mice					133:136	NOD mice	129:136	NOD mice	129:136	A diet enriched in omega-3 PUFA and inulin prevents type 1 diabetes by restoring gut barrier integrity and immune homeostasis in NOD mice.					
36713378	8	82	from	T1D	2366:2368	arg1	models					2385:2390	preclinical models	2373:2390	preclinical models	2373:2390	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	6	83	theme	integrity	1395:1403	arg1	Restoration					1377:1387	Restoration	1377:1387	Restoration of GB integrity	1377:1403	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	8	84	theme	immune	2229:2234	arg1	homeostasis					2236:2246	intestinal immune homeostasis	2218:2246	intestinal immune homeostasis	2218:2246	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	4	85	theme	T1D	1097:1099	arg1	pathogenesis					1101:1112	T1D pathogenesis	1097:1112	T1D pathogenesis	1097:1112	Methods Here we restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA) and tested the effect on T1D pathogenesis.					
36713378	7	86	theme	metabolites	1877:1887	arg1	modifications					1966:1978	modifications	1966:1978	modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila	1966:2137	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	7	86	theme	metabolites	1877:1887	arg1	concentrations					1841:1854	enhanced concentrations	1832:1854	enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways	1832:1960	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	2	87	theme	integrity	485:493	arg1	breakage					470:477	breakage	470:477	breakage of GB integrity	470:493	In support to this idea, we recently demonstrated that breakage of GB integrity leads to activation of islet-reactive T cells and triggers autoimmune Type 1 Diabetes (T1D).					
36713378	6	88	theme	reduced	1463:1469	arg1	expression					1471:1480	reduced expression	1463:1480	reduced expression of IL-1β	1463:1489	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	89	theme	intra-islet	1692:1702	arg1	lymphocytes					1704:1714	intra-islet lymphocytes	1692:1714	intra-islet lymphocytes (IIL)	1692:1720	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	89	theme	intra-islet	1692:1702	arg1	IIL					1717:1719	IIL	1717:1719	IIL	1717:1719	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	8	90	theme	microbial	2313:2321	arg1	profiles					2337:2344	the gut microbial and metabolic profiles	2305:2344	the gut microbial and metabolic profiles	2305:2344	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	3	91	theme	GB	699:700	arg1	alterations					680:690	alterations	680:690	alterations of the GB that specifically affect structure and composition of the mucus layer	680:770	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	0	92	theme	type	52:55	arg1	diabetes					59:66	type 1 diabetes	52:66	type 1 diabetes	52:66	A diet enriched in omega-3 PUFA and inulin prevents type 1 diabetes by restoring gut barrier integrity and immune homeostasis in NOD mice.					
36713378	6	93	theme	T	1549:1549	arg1	cells					1551:1555	regulatory T cells	1538:1555	regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells)	1538:1596	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	7	94	theme	enhanced	1832:1839	arg1	concentrations					1841:1854	enhanced concentrations	1832:1854	enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways	1832:1960	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	8	95	theme	metabolic	2327:2335	arg1	profiles					2337:2344	the gut microbial and metabolic profiles	2305:2344	the gut microbial and metabolic profiles	2305:2344	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	8	96	theme	tolerogenic	2276:2286	arg1	administration					2256:2269	administration	2256:2269	administration of a tolerogenic AID that changed the gut microbial and metabolic profiles	2256:2344	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	8	97	dep	Discussion	2140:2149	arg1	provide					2160:2166	provide	2160:2166	provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models	2160:2390	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	7	98	theme	pathways	1953:1960	arg1	metabolites					1877:1887	anti-inflammatory metabolites	1859:1887	anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways	1859:1960	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	3	99	theme	layer	766:770	arg1	composition					741:751	composition	741:751	composition	741:751	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	3	99	theme	layer	766:770	arg1	structure					727:735	structure	727:735	structure	727:735	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	6	100	from	nodes	1676:1680	arg1	reduction					1419:1427	reduction	1419:1427	reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice	1419:1740	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	100	from	nodes	1676:1680	arg1	intestine					1648:1656	the intestine	1644:1656	the intestine	1644:1656	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	7	101	theme	mucus-modulating	2043:2058	arg1	muciniphila					2098:2108	muciniphila	2098:2108	muciniphila	2098:2108	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	7	101	theme	mucus-modulating	2043:2058	arg1	glycaniphila					2126:2137	glycaniphila	2126:2137	glycaniphila	2126:2137	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	7	101	theme	mucus-modulating	2043:2058	arg1	species					2070:2076	mucus-modulating bacterial species	2043:2076	mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila	2043:2137	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	5	102	theme	NOD	1162:1164	arg1	mice					1166:1169	NOD mice	1162:1169	NOD mice	1162:1169	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	0	103	theme	barrier	85:91	arg1	integrity					93:101	gut barrier integrity	81:101	gut barrier integrity	81:101	A diet enriched in omega-3 PUFA and inulin prevents type 1 diabetes by restoring gut barrier integrity and immune homeostasis in NOD mice.					
36713378	4	104	dep	Methods	909:915	arg1	restored					925:932	restored	925:932	restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA)	925:1070	Methods Here we restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA) and tested the effect on T1D pathogenesis.					
36713378	4	104	dep	Methods	909:915	arg1	tested					1076:1081	tested	1076:1081	tested the effect on T1D pathogenesis	1076:1112	Methods Here we restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA) and tested the effect on T1D pathogenesis.					
36713378	7	105	theme	bacterial	2060:2068	arg1	muciniphila					2098:2108	muciniphila	2098:2108	muciniphila	2098:2108	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	7	105	theme	bacterial	2060:2068	arg1	glycaniphila					2126:2137	glycaniphila	2126:2137	glycaniphila	2126:2137	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	7	105	theme	bacterial	2060:2068	arg1	species					2070:2076	mucus-modulating bacterial species	2043:2076	mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila	2043:2137	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	5	106	theme	immunoregulatory	1287:1302	arg1	mucins					1304:1309	structural (Muc2) and immunoregulatory mucins	1265:1309	structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3)	1265:1325	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	5	106	theme	immunoregulatory	1287:1302	arg1	Muc3					1321:1324	Muc3	1321:1324	Muc3	1321:1324	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	5	106	theme	immunoregulatory	1287:1302	arg1	Muc1					1312:1315	Muc1	1312:1315	Muc1	1312:1315	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	6	107	theme	mice	1737:1740	arg1	reduction					1419:1427	reduction	1419:1427	reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice	1419:1740	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	8	108	theme	preclinical	2373:2383	arg1	models					2385:2390	preclinical models	2373:2390	preclinical models	2373:2390	Discussion Our data provide evidence that the restoration of GB integrity and intestinal immune homeostasis through administration of a tolerogenic AID that changed the gut microbial and metabolic profiles prevents autoimmune T1D in preclinical models.					
36713378	1	109	theme	intestinal	328:337	arg1	level					339:343	the intestinal level	324:343	the intestinal level	324:343	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	6	110	theme	Tr1	1587:1589	arg1	cells					1591:1595	IL-10+ Tr1 cells	1580:1595	IL-10+ Tr1 cells	1580:1595	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	111	theme	AID-fed	1725:1731	arg1	mice					1737:1740	AID-fed NOD mice	1725:1740	AID-fed NOD mice	1725:1740	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	112	theme	intestinal	1432:1441	arg1	inflammation					1443:1454	intestinal inflammation	1432:1454	intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts)	1432:1519	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	5	113	theme	junction	1347:1354	arg1	claudin1					1366:1373	claudin1	1366:1373	claudin1	1366:1373	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	5	113	theme	junction	1347:1354	arg1	proteins					1356:1363	tight junction proteins	1341:1363	tight junction proteins (claudin1)	1341:1374	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	7	114	theme	profile	1998:2004	arg1	modifications					1966:1978	modifications	1966:1978	modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila	1966:2137	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	7	114	theme	profile	1998:2004	arg1	concentrations					1841:1854	enhanced concentrations	1832:1854	enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways	1832:1960	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	6	115	dep	cells	1551:1555	arg1	cells					1591:1595	IL-10+ Tr1 cells	1580:1595	IL-10+ Tr1 cells	1580:1595	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	115	dep	cells	1551:1555	arg1	cells					1570:1574	FoxP3+ Treg cells	1558:1574	FoxP3+ Treg cells	1558:1574	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	116	theme	Treg	1565:1568	arg1	cells					1570:1574	FoxP3+ Treg cells	1558:1574	FoxP3+ Treg cells	1558:1574	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	5	117	theme	mucus	1212:1216	arg1	thickness					1224:1232	increased mucus layer thickness	1202:1232	increased mucus layer thickness	1202:1232	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	1	118	theme	immune	262:267	arg1	system					269:274	the immune system	258:274	the immune system	258:274	Introduction The integrity of the gut barrier (GB) is fundamental to regulate the crosstalk between the microbiota and the immune system and to prevent inflammation and autoimmunity at the intestinal level but also in organs distal from the gut such as the pancreatic islets.					
36713378	3	119	theme	causal	819:824	arg1	link					826:829	a causal link	817:829	a causal link between breakage of the GB integrity and occurrence of autoimmune T1D	817:899	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
36713378	4	120	theme	GB	934:935	arg1	integrity					937:945	GB integrity	934:945	GB integrity	934:945	Methods Here we restored GB integrity in the NOD mice through administration of an anti-inflammatory diet (AID- enriched in soluble fiber inulin and omega 3-PUFA) and tested the effect on T1D pathogenesis.					
36713378	6	121	theme	Th1/Th17	1626:1633	arg1	cells					1635:1639	effector Th1/Th17 cells	1617:1639	effector Th1/Th17 cells	1617:1639	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	122	from	lymphocytes	1704:1714	arg1	reduction					1419:1427	reduction	1419:1427	reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice	1419:1740	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	6	122	from	lymphocytes	1704:1714	arg1	intestine					1648:1656	the intestine	1644:1656	the intestine	1644:1656	Restoration of GB integrity was linked to reduction of intestinal inflammation (i.e., reduced expression of IL-1β, IL-23 and IL-17 transcripts) and expansion of regulatory T cells (FoxP3+ Treg cells and IL-10+ Tr1 cells) at the expenses of effector Th1/Th17 cells in the intestine, pancreatic lymph nodes (PLN) and intra-islet lymphocytes (IIL) of AID-fed NOD mice.					
36713378	5	123	theme	GB	1184:1185	arg1	integrity					1187:1195	GB integrity	1184:1195	GB integrity	1184:1195	Results We found that the AID prevented T1D in NOD mice by restoring GB integrity with increased mucus layer thickness and higher mRNA transcripts of structural (Muc2) and immunoregulatory mucins (Muc1 and Muc3) as well as of tight junction proteins (claudin1).					
36713378	7	124	theme	immune	1792:1797	arg1	homeostasis					1799:1809	immune homeostasis	1792:1809	immune homeostasis	1792:1809	Importantly, the restoration of GB integrity and immune homeostasis were associated with enhanced concentrations of anti-inflammatory metabolites of the ω3/ω6 polyunsaturated fatty acids (PUFA) and arachidonic pathways and modifications of the microbiome profile with increased relative abundance of mucus-modulating bacterial species such as Akkermansia muciniphila and Akkermansia glycaniphila.					
36713378	3	125	theme	GB	855:856	arg1	integrity					858:866	the GB integrity	851:866	the GB integrity	851:866	In T1D patients as in the NOD mice, the spontaneous model of autoimmune diabetes, there are alterations of the GB that specifically affect structure and composition of the mucus layer; however, it is yet to be determined whether a causal link between breakage of the GB integrity and occurrence of autoimmune T1D exists.					
35344548	8	0	theme	unidentified	1518:1529	arg1	cues					1540:1543	unidentified external cues	1518:1543	unidentified external cues in its environment	1518:1562	Collectively, our data provide new insights into how V. cholerae use the VarA-CsrA signaling system to adjust its morphology upon unidentified external cues in its environment.					
35344548	4	1	theme	abnormal	723:730	arg1	morphology					742:751	an abnormal spherical morphology	720:751	an abnormal spherical morphology	720:751	We observed that varA-deficient V. cholerae cells showed an abnormal spherical morphology during late-stage growth.					
35344548	1	2	theme	causative	152:160	arg1	cholerae					138:145	the curve-shaped bacterium Vibrio cholerae	104:145	the curve-shaped bacterium Vibrio cholerae	104:145	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	2	theme	causative	152:160	arg1	agent					162:166	the causative agent	148:166	the causative agent of the diarrheal disease cholera	148:199	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	2	3	theme	virulence	501:509	arg1	regulation					511:520	virulence regulation	501:520	virulence regulation	501:520	This pathway, which mainly signals through the downstream protein CsrA, is highly conserved among gamma-proteobacteria, indicating there is likely a broader function of this system beyond virulence regulation.					
35344548	6	4	from	cells	1123:1127	arg1	overproduction					1059:1072	the CsrA-dependent overproduction	1040:1072	the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool	1040:1178	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	2	5	theme	protein	371:377	arg1	CsrA					379:382	the downstream protein CsrA	356:382	the downstream protein CsrA	356:382	This pathway, which mainly signals through the downstream protein CsrA, is highly conserved among gamma-proteobacteria, indicating there is likely a broader function of this system beyond virulence regulation.					
35344548	8	6	theme	signaling	1471:1479	arg1	system					1481:1486	the VarA-CsrA signaling system	1457:1486	the VarA-CsrA signaling system	1457:1486	Collectively, our data provide new insights into how V. cholerae use the VarA-CsrA signaling system to adjust its morphology upon unidentified external cues in its environment.					
35344548	4	7	dep	V.	695:696	arg1	cholerae					698:705	cholerae	698:705	cholerae	698:705	We observed that varA-deficient V. cholerae cells showed an abnormal spherical morphology during late-stage growth.					
35344548	7	8	theme	cell	1367:1370	arg1	recycling					1377:1385	cell wall recycling	1367:1385	cell wall recycling	1367:1385	Importantly, this phenotype, and the overall cell rounding, could be prevented by means of cell wall recycling.					
35344548	3	9	theme	VarA-CsrA	558:566	arg1	pathway					578:584	the VarA-CsrA signaling pathway	554:584	the VarA-CsrA signaling pathway	554:584	In this study, we investigated the VarA-CsrA signaling pathway and discovered a previously unrecognized link to the shape of the bacterium.					
35344548	6	10	theme	CsrA-dependent	1044:1057	arg1	overproduction					1059:1072	the CsrA-dependent overproduction	1040:1072	the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool	1040:1178	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	5	11	theme	cellular	988:995	arg1	shape					997:1001	the atypical cellular shape	975:1001	the atypical cellular shape	975:1001	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	6	12	theme	precursor	1216:1224	arg1	acid					1257:1260	the PG precursor amino acid meso-diaminopimelic acid	1209:1260	the PG precursor amino acid meso-diaminopimelic acid	1209:1260	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	6	13	theme	acid	1257:1260	arg1	synthesis					1196:1204	the synthesis	1192:1204	the synthesis of the PG precursor amino acid meso-diaminopimelic acid	1192:1260	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	3	14	theme	signaling	568:576	arg1	pathway					578:584	the VarA-CsrA signaling pathway	554:584	the VarA-CsrA signaling pathway	554:584	In this study, we investigated the VarA-CsrA signaling pathway and discovered a previously unrecognized link to the shape of the bacterium.					
35344548	6	15	theme	aspartate	1165:1173	arg1	pool					1175:1178	the cellular aspartate pool	1152:1178	the cellular aspartate pool	1152:1178	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	2	16	theme	downstream	360:369	arg1	CsrA					379:382	the downstream protein CsrA	356:382	the downstream protein CsrA	356:382	This pathway, which mainly signals through the downstream protein CsrA, is highly conserved among gamma-proteobacteria, indicating there is likely a broader function of this system beyond virulence regulation.					
35344548	6	17	theme	PG	1213:1214	arg1	acid					1257:1260	the PG precursor amino acid meso-diaminopimelic acid	1209:1260	the PG precursor amino acid meso-diaminopimelic acid	1209:1260	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	5	18	theme	dipeptides	916:925	arg1	content					892:898	an increased content	879:898	an increased content of disaccharide dipeptides	879:925	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	4	19	theme	varA-deficient	680:693	arg1	cells					707:711	varA-deficient V. cholerae cells	680:711	varA-deficient V. cholerae cells	680:711	We observed that varA-deficient V. cholerae cells showed an abnormal spherical morphology during late-stage growth.					
35344548	5	20	theme	peptidoglycan	787:799	arg1	analyses					818:825	peptidoglycan (PG) composition analyses	787:825	peptidoglycan (PG) composition analyses	787:825	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	6	21	theme	lyase	1095:1099	arg1	overproduction					1059:1072	the CsrA-dependent overproduction	1040:1072	the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool	1040:1178	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	5	22	theme	disaccharide	903:914	arg1	dipeptides					916:925	disaccharide dipeptides	903:925	disaccharide dipeptides	903:925	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	0	23	theme	VarA-CsrA	4:12	arg1	pathway					25:31	The VarA-CsrA regulatory pathway	0:31	The VarA-CsrA regulatory pathway	0:31	The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae.					
35344548	5	24	theme	composition	806:816	arg1	analyses					818:825	peptidoglycan (PG) composition analyses	787:825	peptidoglycan (PG) composition analyses	787:825	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	5	25	with	consistent	959:968	arg1	shape					997:1001	the atypical cellular shape	975:1001	the atypical cellular shape	975:1001	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	1	26	theme	extensive	83:91	arg1	studies					93:99	extensive studies	83:99	extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera	83:199	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	7	27	theme	overall	1313:1319	arg1	rounding					1326:1333	the overall cell rounding	1309:1333	the overall cell rounding	1309:1333	Importantly, this phenotype, and the overall cell rounding, could be prevented by means of cell wall recycling.					
35344548	1	28	theme	diarrheal	175:183	arg1	cholera					193:199	the diarrheal disease cholera	171:199	the diarrheal disease cholera	171:199	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	2	29	theme	broader	462:468	arg1	function					470:477	a broader function	460:477	a broader function of this system beyond virulence regulation	460:520	This pathway, which mainly signals through the downstream protein CsrA, is highly conserved among gamma-proteobacteria, indicating there is likely a broader function of this system beyond virulence regulation.					
35344548	6	30	from	overproduction	1059:1072	arg1	cells					1123:1127	varA mutant cells	1111:1127	varA mutant cells	1111:1127	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	1	31	theme	disease	185:191	arg1	cholera					193:199	the diarrheal disease cholera	171:199	the diarrheal disease cholera	171:199	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	5	32	theme	mutant	853:858	arg1	bacteria					860:867	these mutant bacteria	847:867	these mutant bacteria	847:867	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	7	33	theme	wall	1372:1375	arg1	recycling					1377:1385	cell wall recycling	1367:1385	cell wall recycling	1367:1385	Importantly, this phenotype, and the overall cell rounding, could be prevented by means of cell wall recycling.					
35344548	5	34	contain	contained	869:877	arg2	content					892:898	an increased content	879:898	an increased content of disaccharide dipeptides	879:925	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	5	34	contain	contained	869:877	arg1	bacteria					860:867	these mutant bacteria	847:867	these mutant bacteria	847:867	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	1	35	theme	cholera	193:199	arg1	cholerae					138:145	the curve-shaped bacterium Vibrio cholerae	104:145	the curve-shaped bacterium Vibrio cholerae	104:145	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	35	theme	cholera	193:199	arg1	agent					162:166	the causative agent	148:166	the causative agent of the diarrheal disease cholera	148:199	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	0	36	theme	regulatory	14:23	arg1	pathway					25:31	The VarA-CsrA regulatory pathway	0:31	The VarA-CsrA regulatory pathway	0:31	The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae.					
35344548	6	37	theme	amino	1226:1230	arg1	acid					1257:1260	the PG precursor amino acid meso-diaminopimelic acid	1209:1260	the PG precursor amino acid meso-diaminopimelic acid	1209:1260	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	0	38	theme	cell	44:47	arg1	shape					49:53	cell shape	44:53	cell shape	44:53	The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae.					
35344548	4	39	theme	spherical	732:740	arg1	morphology					742:751	an abnormal spherical morphology	720:751	an abnormal spherical morphology	720:751	We observed that varA-deficient V. cholerae cells showed an abnormal spherical morphology during late-stage growth.					
35344548	8	40	theme	VarA-CsrA	1461:1469	arg1	system					1481:1486	the VarA-CsrA signaling system	1457:1486	the VarA-CsrA signaling system	1457:1486	Collectively, our data provide new insights into how V. cholerae use the VarA-CsrA signaling system to adjust its morphology upon unidentified external cues in its environment.					
35344548	6	41	theme	spherical	1008:1016	arg1	shape					1018:1022	The spherical shape	1004:1022	The spherical shape	1004:1022	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	4	42	theme	V.	695:696	arg1	cells					707:711	varA-deficient V. cholerae cells	680:711	varA-deficient V. cholerae cells	680:711	We observed that varA-deficient V. cholerae cells showed an abnormal spherical morphology during late-stage growth.					
35344548	5	43	theme	increased	882:890	arg1	content					892:898	an increased content	879:898	an increased content of disaccharide dipeptides	879:925	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	2	44	theme	system	487:492	arg1	function					470:477	a broader function	460:477	a broader function of this system beyond virulence regulation	460:520	This pathway, which mainly signals through the downstream protein CsrA, is highly conserved among gamma-proteobacteria, indicating there is likely a broader function of this system beyond virulence regulation.					
35344548	3	45	theme	bacterium	652:660	arg1	shape					639:643	the shape	635:643	the shape of the bacterium	635:660	In this study, we investigated the VarA-CsrA signaling pathway and discovered a previously unrecognized link to the shape of the bacterium.					
35344548	6	46	theme	ammonia	1087:1093	arg1	AspA					1102:1105	AspA	1102:1105	AspA	1102:1105	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	6	46	theme	ammonia	1087:1093	arg1	lyase					1095:1099	aspartate ammonia lyase	1077:1099	aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool	1077:1178	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	1	47	theme	virulence-associated	206:225	arg1	VarS/VarA					279:287	VarS/VarA	279:287	VarS/VarA	279:287	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	47	theme	virulence-associated	206:225	arg1	system					272:277	its virulence-associated regulatory two-component signal transduction system	202:277	its virulence-associated regulatory two-component signal transduction system VarS/VarA	202:287	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	5	48	theme	PG	802:803	arg1	analyses					818:825	peptidoglycan (PG) composition analyses	787:825	peptidoglycan (PG) composition analyses	787:825	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	8	49	from	cues	1540:1543	arg1	environment					1552:1562	its environment	1548:1562	its environment	1548:1562	Collectively, our data provide new insights into how V. cholerae use the VarA-CsrA signaling system to adjust its morphology upon unidentified external cues in its environment.					
35344548	1	50	theme	curve-shaped	108:119	arg1	cholerae					138:145	the curve-shaped bacterium Vibrio cholerae	104:145	the curve-shaped bacterium Vibrio cholerae	104:145	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	50	theme	curve-shaped	108:119	arg1	agent					162:166	the causative agent	148:166	the causative agent of the diarrheal disease cholera	148:199	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	6	51	theme	mutant	1116:1121	arg1	cells					1123:1127	varA mutant cells	1111:1127	varA mutant cells	1111:1127	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	3	52	theme	unrecognized	614:625	arg1	link					627:630	a previously unrecognized link	601:630	a previously unrecognized link to the shape of the bacterium	601:660	In this study, we investigated the VarA-CsrA signaling pathway and discovered a previously unrecognized link to the shape of the bacterium.					
35344548	6	53	from	lyase	1095:1099	arg1	cells					1123:1127	varA mutant cells	1111:1127	varA mutant cells	1111:1127	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	1	54	theme	bacterium	121:129	arg1	cholerae					138:145	the curve-shaped bacterium Vibrio cholerae	104:145	the curve-shaped bacterium Vibrio cholerae	104:145	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	54	theme	bacterium	121:129	arg1	agent					162:166	the causative agent	148:166	the causative agent of the diarrheal disease cholera	148:199	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	6	55	theme	acid	1232:1235	arg1	acid					1257:1260	the PG precursor amino acid meso-diaminopimelic acid	1209:1260	the PG precursor amino acid meso-diaminopimelic acid	1209:1260	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	8	56	theme	new	1419:1421	arg1	insights					1423:1430	new insights	1419:1430	new insights into how V. cholerae use the VarA-CsrA signaling system to adjust its morphology upon unidentified external cues in its environment	1419:1562	Collectively, our data provide new insights into how V. cholerae use the VarA-CsrA signaling system to adjust its morphology upon unidentified external cues in its environment.					
35344548	1	57	theme	Vibrio	131:136	arg1	cholerae					138:145	the curve-shaped bacterium Vibrio cholerae	104:145	the curve-shaped bacterium Vibrio cholerae	104:145	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	57	theme	Vibrio	131:136	arg1	agent					162:166	the causative agent	148:166	the causative agent of the diarrheal disease cholera	148:199	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	58	theme	regulatory	227:236	arg1	VarS/VarA					279:287	VarS/VarA	279:287	VarS/VarA	279:287	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	58	theme	regulatory	227:236	arg1	system					272:277	its virulence-associated regulatory two-component signal transduction system	202:277	its virulence-associated regulatory two-component signal transduction system VarS/VarA	202:287	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	7	59	theme	cell	1321:1324	arg1	rounding					1326:1333	the overall cell rounding	1309:1333	the overall cell rounding	1309:1333	Importantly, this phenotype, and the overall cell rounding, could be prevented by means of cell wall recycling.					
35344548	4	60	theme	late-stage	760:769	arg1	growth					771:776	late-stage growth	760:776	late-stage growth	760:776	We observed that varA-deficient V. cholerae cells showed an abnormal spherical morphology during late-stage growth.					
35344548	5	61	theme	atypical	979:986	arg1	shape					997:1001	the atypical cellular shape	975:1001	the atypical cellular shape	975:1001	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	1	62	theme	two-component	238:250	arg1	VarS/VarA					279:287	VarS/VarA	279:287	VarS/VarA	279:287	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	62	theme	two-component	238:250	arg1	system					272:277	its virulence-associated regulatory two-component signal transduction system	202:277	its virulence-associated regulatory two-component signal transduction system VarS/VarA	202:287	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	8	63	theme	V.	1441:1442	arg1	cholerae					1444:1451	V. cholerae	1441:1451	V. cholerae	1441:1451	Collectively, our data provide new insights into how V. cholerae use the VarA-CsrA signaling system to adjust its morphology upon unidentified external cues in its environment.					
35344548	6	64	theme	meso-diaminopimelic	1237:1255	arg1	acid					1257:1260	the PG precursor amino acid meso-diaminopimelic acid	1209:1260	the PG precursor amino acid meso-diaminopimelic acid	1209:1260	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	6	65	theme	varA	1111:1114	arg1	cells					1123:1127	varA mutant cells	1111:1127	varA mutant cells	1111:1127	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	6	66	theme	cellular	1156:1163	arg1	pool					1175:1178	the cellular aspartate pool	1152:1178	the cellular aspartate pool	1152:1178	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	1	67	from	studies	93:99	arg1	cholerae					138:145	the curve-shaped bacterium Vibrio cholerae	104:145	the curve-shaped bacterium Vibrio cholerae	104:145	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	67	from	studies	93:99	arg1	agent					162:166	the causative agent	148:166	the causative agent of the diarrheal disease cholera	148:199	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	68	theme	signal	252:257	arg1	VarS/VarA					279:287	VarS/VarA	279:287	VarS/VarA	279:287	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	68	theme	signal	252:257	arg1	system					272:277	its virulence-associated regulatory two-component signal transduction system	202:277	its virulence-associated regulatory two-component signal transduction system VarS/VarA	202:287	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	0	69	theme	Vibrio	58:63	arg1	cholerae					65:72	Vibrio cholerae	58:72	Vibrio cholerae	58:72	The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae.					
35344548	8	70	theme	external	1531:1538	arg1	cues					1540:1543	unidentified external cues	1518:1543	unidentified external cues in its environment	1518:1562	Collectively, our data provide new insights into how V. cholerae use the VarA-CsrA signaling system to adjust its morphology upon unidentified external cues in its environment.					
35344548	6	71	theme	aspartate	1077:1085	arg1	AspA					1102:1105	AspA	1102:1105	AspA	1102:1105	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	6	71	theme	aspartate	1077:1085	arg1	lyase					1095:1099	aspartate ammonia lyase	1077:1099	aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool	1077:1178	The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired.					
35344548	5	72	theme	peptide	939:945	arg1	crosslinks					947:956	peptide crosslinks	939:956	peptide crosslinks	939:956	Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape.					
35344548	1	73	theme	transduction	259:270	arg1	VarS/VarA					279:287	VarS/VarA	279:287	VarS/VarA	279:287	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
35344548	1	73	theme	transduction	259:270	arg1	system					272:277	its virulence-associated regulatory two-component signal transduction system	202:277	its virulence-associated regulatory two-component signal transduction system VarS/VarA	202:287	Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.					
36129802	6	0	theme	formula	862:868	arg1	volumes					846:852	Total volumes	840:852	RESULTS Total volumes of term formula purchased	832:878	RESULTS Total volumes of term formula purchased were 216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years.					
36129802	13	1	theme	allergy	1670:1676	arg1	prevalence					1637:1646	the prevalence	1633:1646	the prevalence of cow's milk protein allergy	1633:1676	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	6	2	theme	Total	840:844	arg1	volumes					846:852	Total volumes	840:852	RESULTS Total volumes of term formula purchased	832:878	RESULTS Total volumes of term formula purchased were 216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years.					
36129802	13	3	theme	sold	1754:1757	arg1	sold					1754:1757	all formula sold	1742:1757	all formula sold	1742:1757	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	13	3	theme	sold	1754:1757	arg1	%					1737:1737	4.9% to 7.6%	1726:1737	4.9% to 7.6% of all formula sold	1726:1757	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	8	4	theme	protein	1148:1154	arg1	formula					1156:1162	Soy protein formula	1144:1162	Soy protein formula	1144:1162	Soy protein formula represented 5.1% of formula purchased.					
36129802	14	5	theme	nutritional	1918:1928	arg1	risk					1937:1940	a significant nutritional health risk	1904:1940	a significant nutritional health risk	1904:1940	CONCLUSIONS US infants are exposed to unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula, and this may represent a significant nutritional health risk.					
36129802	2	6	theme	dietary	265:271	arg1	exposures					273:281	these dietary exposures	259:281	these dietary exposures	259:281	The aims of this study were to characterize these dietary exposures in infant formula purchased in the US and to estimate the proportion of formula purchased which is hypoallergenic or lactose-reduced formula.					
36129802	1	7	theme	United	172:177	arg1	States					179:184	the United States	168:184	the United States (US)	168:189	BACKGROUND The protein and carbohydrate composition of formula fed infants' diets in the United States (US) has not been described.					
36129802	7	8	theme	formula	1004:1010	arg1	%					999:999	67.9%	995:999	67.9% of formula purchased	995:1020	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	7	8	theme	formula	1004:1010	arg1	%					1027:1027	26.6%	1023:1027	26.6% was partially hydrolysed	1023:1052	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	7	8	theme	formula	1004:1010	arg1	%					1061:1061	5.5%	1058:1061	5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based)	1058:1141	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	7	8	theme	formula	1004:1010	arg1	formula					1004:1010	formula	1004:1010	formula purchased	1004:1020	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	6	9	theme	formula	903:909	arg1	powder					911:916	formula powder	903:916	formula powder (equivalent to 1.65 billion litres) over 3 years	903:965	RESULTS Total volumes of term formula purchased were 216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years.					
36129802	6	9	theme	formula	903:909	arg1	litres					946:951	equivalent to 1.65 billion litres	919:951	equivalent to 1.65 billion litres	919:951	RESULTS Total volumes of term formula purchased were 216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years.					
36129802	8	10	theme	formula	1184:1190	arg1	formula					1184:1190	formula	1184:1190	formula purchased	1184:1200	Soy protein formula represented 5.1% of formula purchased.					
36129802	8	10	theme	formula	1184:1190	arg1	%					1179:1179	5.1%	1176:1179	5.1% of formula purchased	1176:1200	Soy protein formula represented 5.1% of formula purchased.					
36129802	13	11	theme	hypoallergenic	1577:1590	arg1	formula					1592:1598	hypoallergenic formula	1577:1598	hypoallergenic formula purchased	1577:1608	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	14	12	theme	significant	1906:1916	arg1	risk					1937:1940	a significant nutritional health risk	1904:1940	a significant nutritional health risk	1904:1940	CONCLUSIONS US infants are exposed to unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula, and this may represent a significant nutritional health risk.					
36129802	14	13	dep	CONCLUSIONS	1760:1770	arg1	US					1772:1773	US	1772:1773	US	1772:1773	CONCLUSIONS US infants are exposed to unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula, and this may represent a significant nutritional health risk.					
36129802	1	14	theme	carbohydrate	110:121	arg1	composition					123:133	The protein and carbohydrate composition	94:133	composition	123:133	BACKGROUND The protein and carbohydrate composition of formula fed infants' diets in the United States (US) has not been described.					
36129802	12	15	theme	standard	1451:1458	arg1	formula					1461:1467	'standard' formula	1450:1467	'standard' formula	1450:1467	Of 'standard' formula, defined as intact protein, non-thickened, cow's milk formula, 32.3% was lactose reduced.					
36129802	7	16	theme	protein	975:981	arg1	formula					983:989	Intact protein formula	968:989	Intact protein formula	968:989	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	13	17	theme	formula	1746:1752	arg1	sold					1754:1757	all formula sold	1742:1757	all formula sold	1742:1757	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	4	18	theme	scoop	655:659	arg1	sizes					661:665	Protein and carbohydrate composition and scoop sizes	614:665	sizes	661:665	Protein and carbohydrate composition and scoop sizes for each formula were obtained from manufacturers.					
36129802	13	19	theme	%	1729:1729	arg1	sold					1754:1757	all formula sold	1742:1757	all formula sold	1742:1757	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	13	19	theme	%	1729:1729	arg1	%					1737:1737	4.9% to 7.6%	1726:1737	4.9% to 7.6% of all formula sold	1726:1757	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	0	20	theme	United	68:73	arg1	States					75:80	the United States	64:80	the United States	64:80	Protein and carbohydrate content of infant formula purchased in the United States.					
36129802	6	21	theme	billion	938:944	arg1	powder					911:916	formula powder	903:916	formula powder (equivalent to 1.65 billion litres) over 3 years	903:965	RESULTS Total volumes of term formula purchased were 216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years.					
36129802	6	21	theme	billion	938:944	arg1	litres					946:951	equivalent to 1.65 billion litres	919:951	equivalent to 1.65 billion litres	919:951	RESULTS Total volumes of term formula purchased were 216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years.					
36129802	9	22	dep	%	1240:1240	arg1	lactose					1242:1248	lactose	1242:1248	52.7% lactose	1236:1248	Carbohydrate content overall was 52.7% lactose, 42.3% glucose polymers and 5.0% sucrose.					
36129802	7	23	dep	%	1086:1086	arg1	protein					1111:1117	extensively hydrolysed protein	1088:1117	5.2% extensively hydrolysed protein; 0.3% amino acid based	1083:1140	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	7	23	dep	%	1086:1086	arg1	%					1123:1123	0.3%	1120:1123	5.2% extensively hydrolysed protein; 0.3% amino acid based	1083:1140	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	9	24	theme	Carbohydrate	1203:1214	arg1	content					1216:1222	Carbohydrate content	1203:1222	Carbohydrate content	1203:1222	Carbohydrate content overall was 52.7% lactose, 42.3% glucose polymers and 5.0% sucrose.					
36129802	13	25	theme	study	1708:1712	arg1	period					1714:1719	the 3-year study period	1697:1719	the 3-year study period	1697:1719	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	0	26	theme	infant	36:41	arg1	formula					43:49	infant formula	36:49	infant formula	36:49	Protein and carbohydrate content of infant formula purchased in the United States.					
36129802	14	27	theme	non-lactose	1827:1837	arg1	carbohydrates					1839:1851	non-lactose carbohydrates	1827:1851	non-lactose carbohydrates	1827:1851	CONCLUSIONS US infants are exposed to unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula, and this may represent a significant nutritional health risk.					
36129802	12	28	theme	milk	1518:1521	arg1	formula					1523:1529	cow's milk formula	1512:1529	cow's milk formula	1512:1529	Of 'standard' formula, defined as intact protein, non-thickened, cow's milk formula, 32.3% was lactose reduced.					
36129802	12	28	theme	milk	1518:1521	arg1	protein					1488:1494	intact protein	1481:1494	intact protein	1481:1494	Of 'standard' formula, defined as intact protein, non-thickened, cow's milk formula, 32.3% was lactose reduced.					
36129802	2	29	theme	lactose-reduced	400:414	arg1	proportion					341:350	the proportion	337:350	the proportion of formula purchased which is hypoallergenic or lactose-reduced formula	337:422	The aims of this study were to characterize these dietary exposures in infant formula purchased in the US and to estimate the proportion of formula purchased which is hypoallergenic or lactose-reduced formula.					
36129802	2	29	theme	lactose-reduced	400:414	arg1	formula					416:422	hypoallergenic or lactose-reduced formula	382:422	hypoallergenic or lactose-reduced formula	382:422	The aims of this study were to characterize these dietary exposures in infant formula purchased in the US and to estimate the proportion of formula purchased which is hypoallergenic or lactose-reduced formula.					
36129802	9	30	dep	%	1255:1255	arg1	polymers					1265:1272	glucose polymers	1257:1272	42.3% glucose polymers	1251:1272	Carbohydrate content overall was 52.7% lactose, 42.3% glucose polymers and 5.0% sucrose.					
36129802	14	31	theme	health	1930:1935	arg1	risk					1937:1940	a significant nutritional health risk	1904:1940	a significant nutritional health risk	1904:1940	CONCLUSIONS US infants are exposed to unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula, and this may represent a significant nutritional health risk.					
36129802	5	32	theme	premature	762:770	arg1	infants					772:778	premature infants	762:778	premature infants	762:778	Ready to feed liquid products, products for premature infants and products for over 1 year old were not included.					
36129802	9	33	theme	52.7	1236:1239	arg1	%					1240:1240	52.7%	1236:1240	52.7% lactose	1236:1248	Carbohydrate content overall was 52.7% lactose, 42.3% glucose polymers and 5.0% sucrose.					
36129802	2	34	theme	hypoallergenic	382:395	arg1	proportion					341:350	the proportion	337:350	the proportion of formula purchased which is hypoallergenic or lactose-reduced formula	337:422	The aims of this study were to characterize these dietary exposures in infant formula purchased in the US and to estimate the proportion of formula purchased which is hypoallergenic or lactose-reduced formula.					
36129802	2	34	theme	hypoallergenic	382:395	arg1	formula					416:422	hypoallergenic or lactose-reduced formula	382:422	hypoallergenic or lactose-reduced formula	382:422	The aims of this study were to characterize these dietary exposures in infant formula purchased in the US and to estimate the proportion of formula purchased which is hypoallergenic or lactose-reduced formula.					
36129802	7	35	dep	%	1123:1123	arg1	based					1136:1140	amino acid based	1125:1140	5.2% extensively hydrolysed protein; 0.3% amino acid based	1083:1140	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	0	36	theme	carbohydrate	12:23	arg1	content					25:31	carbohydrate content	12:31	carbohydrate content	12:31	Protein and carbohydrate content of infant formula purchased in the United States.					
36129802	1	37	theme	protein	98:104	arg1	composition					123:133	The protein and carbohydrate composition	94:133	composition	123:133	BACKGROUND The protein and carbohydrate composition of formula fed infants' diets in the United States (US) has not been described.					
36129802	2	38	theme	formula	355:361	arg1	proportion					341:350	the proportion	337:350	the proportion of formula purchased which is hypoallergenic or lactose-reduced formula	337:422	The aims of this study were to characterize these dietary exposures in infant formula purchased in the US and to estimate the proportion of formula purchased which is hypoallergenic or lactose-reduced formula.					
36129802	2	38	theme	formula	355:361	arg1	formula					416:422	hypoallergenic or lactose-reduced formula	382:422	hypoallergenic or lactose-reduced formula	382:422	The aims of this study were to characterize these dietary exposures in infant formula purchased in the US and to estimate the proportion of formula purchased which is hypoallergenic or lactose-reduced formula.					
36129802	3	39	theme	purchase	457:464	arg1	data					466:469	Powdered infant formula purchase data	433:469	Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019,	433:565	METHODS Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc.					
36129802	0	40	theme	formula	43:49	arg1	Protein					0:6	Protein	0:6	Protein	0:6	Protein and carbohydrate content of infant formula purchased in the United States.					
36129802	0	40	theme	formula	43:49	arg1	content					25:31	carbohydrate content	12:31	carbohydrate content	12:31	Protein and carbohydrate content of infant formula purchased in the United States.					
36129802	9	41	theme	glucose	1257:1263	arg1	polymers					1265:1272	glucose polymers	1257:1272	42.3% glucose polymers	1251:1272	Carbohydrate content overall was 52.7% lactose, 42.3% glucose polymers and 5.0% sucrose.					
36129802	2	42	theme	infant	286:291	arg1	formula					293:299	infant formula	286:299	infant formula purchased in the US	286:319	The aims of this study were to characterize these dietary exposures in infant formula purchased in the US and to estimate the proportion of formula purchased which is hypoallergenic or lactose-reduced formula.					
36129802	1	43	dep	States	179:184	arg1	US					187:188	US	187:188	US	187:188	BACKGROUND The protein and carbohydrate composition of formula fed infants' diets in the United States (US) has not been described.					
36129802	3	44	theme	major	480:484	arg1	stores					495:500	all major physical stores	476:500	all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019,	476:565	METHODS Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc.					
36129802	9	45	theme	%	1281:1281	arg1	sucrose					1283:1289	5.0% sucrose	1278:1289	5.0% sucrose	1278:1289	Carbohydrate content overall was 52.7% lactose, 42.3% glucose polymers and 5.0% sucrose.					
36129802	13	46	theme	protein	1662:1668	arg1	allergy					1670:1676	cow's milk protein allergy	1651:1676	cow's milk protein allergy	1651:1676	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	6	47	theme	term	857:860	arg1	formula					862:868	term formula	857:868	term formula purchased	857:878	RESULTS Total volumes of term formula purchased were 216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years.					
36129802	13	48	dep	%	1737:1737	arg1	to					1731:1732	to	1731:1732	to	1731:1732	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	2	49	theme	study	232:236	arg1	aims					219:222	The aims	215:222	The aims of this study	215:236	The aims of this study were to characterize these dietary exposures in infant formula purchased in the US and to estimate the proportion of formula purchased which is hypoallergenic or lactose-reduced formula.					
36129802	13	50	theme	formula	1592:1598	arg1	proportion					1563:1572	The proportion	1559:1572	The proportion of hypoallergenic formula purchased	1559:1608	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	5	51	theme	feed	727:730	arg1	products					739:746	feed liquid products	727:746	feed liquid products	727:746	Ready to feed liquid products, products for premature infants and products for over 1 year old were not included.					
36129802	7	52	theme	amino	1125:1129	arg1	based					1136:1140	amino acid based	1125:1140	5.2% extensively hydrolysed protein; 0.3% amino acid based	1083:1140	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	12	53	dep	reduced	1550:1556	arg1	lactose					1542:1548	lactose	1542:1548	lactose	1542:1548	Of 'standard' formula, defined as intact protein, non-thickened, cow's milk formula, 32.3% was lactose reduced.					
36129802	8	54	theme	Soy	1144:1146	arg1	formula					1156:1162	Soy protein formula	1144:1162	Soy protein formula	1144:1162	Soy protein formula represented 5.1% of formula purchased.					
36129802	11	55	theme	non-lactose	1421:1431	arg1	carbohydrate					1433:1444	a non-lactose carbohydrate	1419:1444	a non-lactose carbohydrate	1419:1444	Of all formula purchased, 59.0% was lactose reduced, containing a non-lactose carbohydrate.					
36129802	3	56	theme	COVID-19	525:532	arg1	pandemic					534:541	the COVID-19 pandemic	521:541	the COVID-19 pandemic	521:541	METHODS Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc.					
36129802	6	57	theme	powder	911:916	arg1	kg					897:898	216 million kg	885:898	216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years	885:965	RESULTS Total volumes of term formula purchased were 216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years.					
36129802	4	58	theme	Protein	614:620	arg1	composition					639:649	Protein and carbohydrate composition and scoop sizes	614:665	composition	639:649	Protein and carbohydrate composition and scoop sizes for each formula were obtained from manufacturers.					
36129802	14	59	dep	US	1772:1773	arg1	infants					1775:1781	infants	1775:1781	CONCLUSIONS US infants	1760:1781	CONCLUSIONS US infants are exposed to unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula, and this may represent a significant nutritional health risk.					
36129802	6	60	theme	216 million	885:895	arg1	kg					897:898	216 million kg	885:898	216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years	885:965	RESULTS Total volumes of term formula purchased were 216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years.					
36129802	7	61	theme	Intact	968:973	arg1	formula					983:989	Intact protein formula	968:989	Intact protein formula	968:989	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	3	62	theme	infant	442:447	arg1	data					466:469	Powdered infant formula purchase data	433:469	Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019,	433:565	METHODS Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc.					
36129802	7	63	theme	acid	1131:1134	arg1	based					1136:1140	amino acid based	1125:1140	5.2% extensively hydrolysed protein; 0.3% amino acid based	1083:1140	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	1	64	theme	formula	138:144	arg1	composition					123:133	The protein and carbohydrate composition	94:133	composition	123:133	BACKGROUND The protein and carbohydrate composition of formula fed infants' diets in the United States (US) has not been described.					
36129802	6	65	dep	1.65	933:936	arg1	to					930:931	to	930:931	to	930:931	RESULTS Total volumes of term formula purchased were 216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years.					
36129802	4	66	theme	carbohydrate	626:637	arg1	composition					639:649	Protein and carbohydrate composition and scoop sizes	614:665	composition	639:649	Protein and carbohydrate composition and scoop sizes for each formula were obtained from manufacturers.					
36129802	3	67	from	stores	495:500	arg1	US					509:510	US	509:510	US	509:510	METHODS Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc.					
36129802	3	67	from	stores	495:500	arg1	data					466:469	Powdered infant formula purchase data	433:469	Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019,	433:565	METHODS Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc.					
36129802	1	68	dep	BACKGROUND	83:92	arg1	described					204:212	described	204:212	has not been described	191:212	BACKGROUND The protein and carbohydrate composition of formula fed infants' diets in the United States (US) has not been described.					
36129802	14	69	theme	hypoallergenic	1857:1870	arg1	formula					1872:1878	hypoallergenic formula	1857:1878	hypoallergenic formula	1857:1878	CONCLUSIONS US infants are exposed to unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula, and this may represent a significant nutritional health risk.					
36129802	10	70	theme	formula	1301:1307	arg1	formula					1301:1307	formula	1301:1307	formula purchased	1301:1317	23.7% of formula purchased included sucrose as a carbohydrate.					
36129802	10	70	theme	formula	1301:1307	arg1	%					1296:1296	23.7%	1292:1296	23.7% of formula purchased	1292:1317	23.7% of formula purchased included sucrose as a carbohydrate.					
36129802	13	71	theme	3-year	1701:1706	arg1	period					1714:1719	the 3-year study period	1697:1719	the 3-year study period	1697:1719	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	7	72	theme	hydrolysed	1100:1109	arg1	protein					1111:1117	extensively hydrolysed protein	1088:1117	5.2% extensively hydrolysed protein; 0.3% amino acid based	1083:1140	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	14	73	theme	carbohydrates	1839:1851	arg1	levels					1817:1822	unnecessarily high levels	1798:1822	unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula	1798:1878	CONCLUSIONS US infants are exposed to unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula, and this may represent a significant nutritional health risk.					
36129802	7	74	dep	hypoallergenic	1067:1080	arg1	%					1086:1086	5.2%	1083:1086	5.2% extensively hydrolysed protein; 0.3% amino acid based	1083:1140	Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based).					
36129802	14	75	theme	high	1812:1815	arg1	levels					1817:1822	unnecessarily high levels	1798:1822	unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula	1798:1878	CONCLUSIONS US infants are exposed to unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula, and this may represent a significant nutritional health risk.					
36129802	12	76	theme	intact	1481:1486	arg1	formula					1523:1529	cow's milk formula	1512:1529	cow's milk formula	1512:1529	Of 'standard' formula, defined as intact protein, non-thickened, cow's milk formula, 32.3% was lactose reduced.					
36129802	12	76	theme	intact	1481:1486	arg1	non-thickened					1497:1509	non-thickened	1497:1509	non-thickened	1497:1509	Of 'standard' formula, defined as intact protein, non-thickened, cow's milk formula, 32.3% was lactose reduced.					
36129802	12	76	theme	intact	1481:1486	arg1	protein					1488:1494	intact protein	1481:1494	intact protein	1481:1494	Of 'standard' formula, defined as intact protein, non-thickened, cow's milk formula, 32.3% was lactose reduced.					
36129802	3	77	theme	Information	586:596	arg1	Inc.					609:612	Inc.	609:612	Inc.	609:612	METHODS Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc.					
36129802	3	77	theme	Information	586:596	arg1	Resources					598:606	Information Resources	586:606	Information Resources	586:606	METHODS Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc.					
36129802	3	78	theme	Powdered	433:440	arg1	data					466:469	Powdered infant formula purchase data	433:469	Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019,	433:565	METHODS Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc.					
36129802	5	79	theme	liquid	732:737	arg1	products					739:746	feed liquid products	727:746	feed liquid products	727:746	Ready to feed liquid products, products for premature infants and products for over 1 year old were not included.					
36129802	11	80	dep	reduced	1399:1405	arg1	lactose					1391:1397	lactose	1391:1397	lactose	1391:1397	Of all formula purchased, 59.0% was lactose reduced, containing a non-lactose carbohydrate.					
36129802	6	81	dep	RESULTS	832:838	arg1	volumes					846:852	Total volumes	840:852	RESULTS Total volumes of term formula purchased	832:878	RESULTS Total volumes of term formula purchased were 216 million kg of formula powder (equivalent to 1.65 billion litres) over 3 years.					
36129802	14	82	theme	formula	1872:1878	arg1	levels					1817:1822	unnecessarily high levels	1798:1822	unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula	1798:1878	CONCLUSIONS US infants are exposed to unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula, and this may represent a significant nutritional health risk.					
36129802	3	83	theme	formula	449:455	arg1	data					466:469	Powdered infant formula purchase data	433:469	Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019,	433:565	METHODS Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc.					
36129802	9	84	theme	5.0	1278:1280	arg1	%					1281:1281	%	1281:1281	%	1281:1281	Carbohydrate content overall was 52.7% lactose, 42.3% glucose polymers and 5.0% sucrose.					
36129802	3	85	dep	METHODS	425:431	arg1	obtained					572:579	obtained	572:579	were obtained from Information Resources, Inc.	567:612	METHODS Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc.					
36129802	13	86	theme	milk	1657:1660	arg1	allergy					1670:1676	cow's milk protein allergy	1651:1676	cow's milk protein allergy	1651:1676	The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold.					
36129802	5	87	dep	for	793:795	arg1	over					797:800	over	797:800	over	797:800	Ready to feed liquid products, products for premature infants and products for over 1 year old were not included.					
36129802	3	88	theme	physical	486:493	arg1	stores					495:500	all major physical stores	476:500	all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019,	476:565	METHODS Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc.					
36067567	8	0	theme	host	1843:1846	arg1	response					1848:1855	host response	1843:1855	host response	1843:1855	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	3	1	theme	vascular	788:795	arg1	VEGF					824:827	VEGF	824:827	VEGF	824:827	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	3	1	theme	vascular	788:795	arg1	factor					816:821	vascular endothelial growth factor	788:821	vascular endothelial growth factor (VEGF)	788:828	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	6	2	theme	immune	1415:1420	arg1	response					1422:1429	the immune response	1411:1429	the immune response	1411:1429	Subcutaneous implantation in mice suggested that the composite could steer the immune response toward an anti-inflammation state and subcutaneous implantation in rats suggested an anti-calcification effect of it.					
36067567	5	3	theme	balance	1327:1333	arg1	auto-regulation					1304:1318	auto-regulation	1304:1318	auto-regulation of ROS balance	1304:1333	In addition, the composite could sense oxidation biosignal present in the inflammation environment and subsequently release a ROS scavenger for auto-regulation of ROS balance.					
36067567	7	4	theme	endovascular	1649:1660	arg1	implantation					1662:1673	the endovascular implantation	1645:1673	the endovascular implantation of tissues	1645:1684	The enhanced hemocompatibility and endothelialization effects in vivo were further confirmed by the endovascular implantation of tissues via membrane-covered stent delivery.					
36067567	7	5	theme	in	1611:1612	arg1	effects					1603:1609	The enhanced hemocompatibility and endothelialization effects	1549:1609	The enhanced hemocompatibility and endothelialization effects in vivo	1549:1617	The enhanced hemocompatibility and endothelialization effects in vivo were further confirmed by the endovascular implantation of tissues via membrane-covered stent delivery.					
36067567	1	6	theme	premature	319:327	arg1	inflammation					358:369	inflammation	358:369	inflammation	358:369	Cardiovascular implants made from heterogeneous tissues (HT) often clinically face premature failures such as thrombosis, inflammation, and calcification.					
36067567	1	6	theme	premature	319:327	arg1	failures					329:336	premature failures	319:336	premature failures such as thrombosis, inflammation, and calcification	319:388	Cardiovascular implants made from heterogeneous tissues (HT) often clinically face premature failures such as thrombosis, inflammation, and calcification.					
36067567	1	6	theme	premature	319:327	arg1	thrombosis					346:355	thrombosis	346:355	thrombosis	346:355	Cardiovascular implants made from heterogeneous tissues (HT) often clinically face premature failures such as thrombosis, inflammation, and calcification.					
36067567	1	6	theme	premature	319:327	arg1	calcification					376:388	calcification	376:388	calcification	376:388	Cardiovascular implants made from heterogeneous tissues (HT) often clinically face premature failures such as thrombosis, inflammation, and calcification.					
36067567	7	7	theme	endothelialization	1584:1601	arg1	effects					1603:1609	The enhanced hemocompatibility and endothelialization effects	1549:1609	The enhanced hemocompatibility and endothelialization effects in vivo	1549:1617	The enhanced hemocompatibility and endothelialization effects in vivo were further confirmed by the endovascular implantation of tissues via membrane-covered stent delivery.					
36067567	7	8	theme	hemocompatibility	1562:1578	arg1	effects					1603:1609	The enhanced hemocompatibility and endothelialization effects	1549:1609	The enhanced hemocompatibility and endothelialization effects in vivo	1549:1617	The enhanced hemocompatibility and endothelialization effects in vivo were further confirmed by the endovascular implantation of tissues via membrane-covered stent delivery.					
36067567	3	9	theme	segment-crosslinked	664:682	arg1	heparin					684:690	MMP-responsive segment-crosslinked heparin	649:690	MMP-responsive segment-crosslinked heparin mimicking polymer	649:708	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	8	10	theme	anticoagulant	1942:1954	arg1	functions					2019:2027	the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions	1938:2027	the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants	1938:2063	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	1	11	theme	Cardiovascular	236:249	arg1	implants					251:258	Cardiovascular implants	236:258	Cardiovascular implants made from heterogeneous tissues (HT)	236:295	Cardiovascular implants made from heterogeneous tissues (HT) often clinically face premature failures such as thrombosis, inflammation, and calcification.					
36067567	6	12	dep	state	1459:1463	arg1	an					1438:1439	an	1438:1439	an	1438:1439	Subcutaneous implantation in mice suggested that the composite could steer the immune response toward an anti-inflammation state and subcutaneous implantation in rats suggested an anti-calcification effect of it.					
36067567	4	13	theme	heparin	1028:1034	arg1	release					1017:1023	the release	1013:1023	the release of heparin mimicking polymer and VEGF	1013:1061	Matrix metalloproteinase (MMP), which reportedly showed elevated expression in inflammation response to foreign implant degraded the hydrogel and led to the release of heparin mimicking polymer and VEGF, enhancing its anti-coagulation capacity and accelerating the growth of endothelial cells on it.					
36067567	3	14	theme	electrostatic	834:846	arg1	attraction					848:857	electrostatic attraction	834:857	electrostatic attraction	834:857	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	8	15	theme	endothelialization	1957:1974	arg1	functions					2019:2027	the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions	1938:2027	the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants	1938:2063	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	0	16	from	heparin	80:86	arg1	release					28:34	Inflammation-triggered dual release	0:34	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin	0:86	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin mimicking hydrogel-tissue composite as cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification.					
36067567	8	17	theme	anti-inflammatory	1977:1993	arg1	functions					2019:2027	the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions	1938:2027	the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants	1938:2063	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	1	18	theme	heterogeneous	270:282	arg1	HT					293:294	HT	293:294	HT	293:294	Cardiovascular implants made from heterogeneous tissues (HT) often clinically face premature failures such as thrombosis, inflammation, and calcification.					
36067567	1	18	theme	heterogeneous	270:282	arg1	tissues					284:290	heterogeneous tissues	270:290	heterogeneous tissues (HT)	270:295	Cardiovascular implants made from heterogeneous tissues (HT) often clinically face premature failures such as thrombosis, inflammation, and calcification.					
36067567	6	19	theme	anti-inflammation	1441:1457	arg1	state					1459:1463	anti-inflammation state	1441:1463	anti-inflammation state	1441:1463	Subcutaneous implantation in mice suggested that the composite could steer the immune response toward an anti-inflammation state and subcutaneous implantation in rats suggested an anti-calcification effect of it.					
36067567	4	20	from	expression	925:934	arg1	response					952:959	inflammation response	939:959	inflammation response to foreign implant	939:978	Matrix metalloproteinase (MMP), which reportedly showed elevated expression in inflammation response to foreign implant degraded the hydrogel and led to the release of heparin mimicking polymer and VEGF, enhancing its anti-coagulation capacity and accelerating the growth of endothelial cells on it.					
36067567	6	21	theme	Subcutaneous	1336:1347	arg1	implantation					1349:1360	Subcutaneous implantation	1336:1360	Subcutaneous implantation in mice	1336:1368	Subcutaneous implantation in mice suggested that the composite could steer the immune response toward an anti-inflammation state and subcutaneous implantation in rats suggested an anti-calcification effect of it.					
36067567	6	22	from	implantation	1349:1360	arg1	mice					1365:1368	mice	1365:1368	mice	1365:1368	Subcutaneous implantation in mice suggested that the composite could steer the immune response toward an anti-inflammation state and subcutaneous implantation in rats suggested an anti-calcification effect of it.					
36067567	3	23	theme	nitroxide	728:736	arg1	radical					738:744	a nitroxide radical	726:744	a nitroxide radical	726:744	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	4	24	theme	anti-coagulation	1078:1093	arg1	capacity					1095:1102	its anti-coagulation capacity	1074:1102	its anti-coagulation capacity	1074:1102	Matrix metalloproteinase (MMP), which reportedly showed elevated expression in inflammation response to foreign implant degraded the hydrogel and led to the release of heparin mimicking polymer and VEGF, enhancing its anti-coagulation capacity and accelerating the growth of endothelial cells on it.					
36067567	6	25	theme	it	1545:1546	arg1	effect					1535:1540	an anti-calcification effect	1513:1540	an anti-calcification effect of it	1513:1546	Subcutaneous implantation in mice suggested that the composite could steer the immune response toward an anti-inflammation state and subcutaneous implantation in rats suggested an anti-calcification effect of it.					
36067567	8	26	theme	HT-based	2032:2039	arg1	implants					2056:2063	HT-based cardiovascular implants	2032:2063	HT-based cardiovascular implants	2032:2063	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	8	27	theme	implants	2056:2063	arg1	functions					2019:2027	the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions	1938:2027	the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants	1938:2063	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	0	28	theme	hydrogel-tissue	98:112	arg1	composite					114:122	hydrogel-tissue composite	98:122	hydrogel-tissue composite	98:122	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin mimicking hydrogel-tissue composite as cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification.					
36067567	0	28	theme	hydrogel-tissue	98:112	arg1	implants					142:149	cardiovascular implants	127:149	cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification	127:233	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin mimicking hydrogel-tissue composite as cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification.					
36067567	5	29	theme	oxidation	1199:1207	arg1	biosignal					1209:1217	oxidation biosignal	1199:1217	oxidation biosignal present in the inflammation environment	1199:1257	In addition, the composite could sense oxidation biosignal present in the inflammation environment and subsequently release a ROS scavenger for auto-regulation of ROS balance.					
36067567	2	30	theme	instructive	461:471	arg1	release					473:479	inflammation instructive release	448:479	inflammation instructive release of multiple components	448:502	Herein, we report a hydrogel-tissue composite exhibiting inflammation instructive release of multiple components towards preventing coagulation, promoting endothelial growth, and modulating reactive oxygen species (ROS) homeostasis.					
36067567	5	31	theme	present	1219:1225	arg1	biosignal					1209:1217	oxidation biosignal	1199:1217	oxidation biosignal present in the inflammation environment	1199:1257	In addition, the composite could sense oxidation biosignal present in the inflammation environment and subsequently release a ROS scavenger for auto-regulation of ROS balance.					
36067567	0	32	theme	radical	49:55	arg1	release					28:34	Inflammation-triggered dual release	0:34	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin	0:86	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin mimicking hydrogel-tissue composite as cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification.					
36067567	4	33	theme	cells	1147:1151	arg1	growth					1125:1130	the growth	1121:1130	the growth of endothelial cells on it	1121:1157	Matrix metalloproteinase (MMP), which reportedly showed elevated expression in inflammation response to foreign implant degraded the hydrogel and led to the release of heparin mimicking polymer and VEGF, enhancing its anti-coagulation capacity and accelerating the growth of endothelial cells on it.					
36067567	2	34	theme	oxygen	590:595	arg1	ROS					606:608	ROS	606:608	ROS	606:608	Herein, we report a hydrogel-tissue composite exhibiting inflammation instructive release of multiple components towards preventing coagulation, promoting endothelial growth, and modulating reactive oxygen species (ROS) homeostasis.					
36067567	2	34	theme	oxygen	590:595	arg1	species					597:603	reactive oxygen species	581:603	reactive oxygen species (ROS) homeostasis	581:621	Herein, we report a hydrogel-tissue composite exhibiting inflammation instructive release of multiple components towards preventing coagulation, promoting endothelial growth, and modulating reactive oxygen species (ROS) homeostasis.					
36067567	0	35	theme	Inflammation-triggered	0:21	arg1	release					28:34	Inflammation-triggered dual release	0:34	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin	0:86	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin mimicking hydrogel-tissue composite as cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification.					
36067567	4	36	theme	Matrix	860:865	arg1	MMP					886:888	MMP	886:888	MMP	886:888	Matrix metalloproteinase (MMP), which reportedly showed elevated expression in inflammation response to foreign implant degraded the hydrogel and led to the release of heparin mimicking polymer and VEGF, enhancing its anti-coagulation capacity and accelerating the growth of endothelial cells on it.					
36067567	4	36	theme	Matrix	860:865	arg1	metalloproteinase					867:883	Matrix metalloproteinase	860:883	Matrix metalloproteinase (MMP)	860:889	Matrix metalloproteinase (MMP), which reportedly showed elevated expression in inflammation response to foreign implant degraded the hydrogel and led to the release of heparin mimicking polymer and VEGF, enhancing its anti-coagulation capacity and accelerating the growth of endothelial cells on it.					
36067567	2	37	theme	components	493:502	arg1	release					473:479	inflammation instructive release	448:479	inflammation instructive release of multiple components	448:502	Herein, we report a hydrogel-tissue composite exhibiting inflammation instructive release of multiple components towards preventing coagulation, promoting endothelial growth, and modulating reactive oxygen species (ROS) homeostasis.					
36067567	3	38	theme	cleavable	754:762	arg1	bonds					778:782	ROS cleavable boronic ester bonds	750:782	ROS cleavable boronic ester bonds	750:782	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	0	39	theme	nitroxide	39:47	arg1	radical					49:55	nitroxide radical	39:55	nitroxide radical	39:55	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin mimicking hydrogel-tissue composite as cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification.					
36067567	2	40	theme	endothelial	546:556	arg1	growth					558:563	endothelial growth	546:563	endothelial growth	546:563	Herein, we report a hydrogel-tissue composite exhibiting inflammation instructive release of multiple components towards preventing coagulation, promoting endothelial growth, and modulating reactive oxygen species (ROS) homeostasis.					
36067567	0	41	theme	factor	68:73	arg1	release					28:34	Inflammation-triggered dual release	0:34	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin	0:86	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin mimicking hydrogel-tissue composite as cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification.					
36067567	4	42	theme	inflammation	939:950	arg1	response					952:959	inflammation response	939:959	inflammation response to foreign implant	939:978	Matrix metalloproteinase (MMP), which reportedly showed elevated expression in inflammation response to foreign implant degraded the hydrogel and led to the release of heparin mimicking polymer and VEGF, enhancing its anti-coagulation capacity and accelerating the growth of endothelial cells on it.					
36067567	6	43	from	state	1459:1463	arg1	rats					1498:1501	rats	1498:1501	rats suggested an anti-calcification effect of it	1498:1546	Subcutaneous implantation in mice suggested that the composite could steer the immune response toward an anti-inflammation state and subcutaneous implantation in rats suggested an anti-calcification effect of it.					
36067567	7	44	theme	membrane-covered	1690:1705	arg1	delivery					1713:1720	membrane-covered stent delivery	1690:1720	membrane-covered stent delivery	1690:1720	The enhanced hemocompatibility and endothelialization effects in vivo were further confirmed by the endovascular implantation of tissues via membrane-covered stent delivery.					
36067567	8	45	theme	functional	1782:1791	arg1	hydrogel					1793:1800	functional hydrogel	1782:1800	functional hydrogel	1782:1800	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	8	46	theme	controlled	1861:1870	arg1	release					1872:1878	controlled release	1861:1878	controlled release of multiple active cargos	1861:1904	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	7	47	theme	tissues	1678:1684	arg1	implantation					1662:1673	the endovascular implantation	1645:1673	the endovascular implantation of tissues	1645:1684	The enhanced hemocompatibility and endothelialization effects in vivo were further confirmed by the endovascular implantation of tissues via membrane-covered stent delivery.					
36067567	3	48	theme	ester	772:776	arg1	bonds					778:782	ROS cleavable boronic ester bonds	750:782	ROS cleavable boronic ester bonds	750:782	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	2	49	theme	hydrogel-tissue	411:425	arg1	composite					427:435	a hydrogel-tissue composite	409:435	a hydrogel-tissue composite exhibiting inflammation instructive release of multiple components towards preventing coagulation, promoting endothelial growth, and modulating reactive oxygen species (ROS) homeostasis	409:621	Herein, we report a hydrogel-tissue composite exhibiting inflammation instructive release of multiple components towards preventing coagulation, promoting endothelial growth, and modulating reactive oxygen species (ROS) homeostasis.					
36067567	8	50	theme	tissue	1811:1816	arg1	sophistically					1818:1830	the tissue sophistically	1807:1830	the tissue sophistically exploiting host response for controlled release of multiple active cargos	1807:1904	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	8	51	theme	active	1892:1897	arg1	cargos					1899:1904	multiple active cargos	1883:1904	multiple active cargos	1883:1904	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	5	52	theme	ROS	1323:1325	arg1	balance					1327:1333	ROS balance	1323:1333	ROS balance	1323:1333	In addition, the composite could sense oxidation biosignal present in the inflammation environment and subsequently release a ROS scavenger for auto-regulation of ROS balance.					
36067567	5	53	attach	present	1219:1225	arg1	environment					1247:1257	the inflammation environment	1230:1257	the inflammation environment	1230:1257	In addition, the composite could sense oxidation biosignal present in the inflammation environment and subsequently release a ROS scavenger for auto-regulation of ROS balance.					
36067567	5	53	attach	present	1219:1225	arg2	biosignal					1209:1217	oxidation biosignal	1199:1217	oxidation biosignal present in the inflammation environment	1199:1257	In addition, the composite could sense oxidation biosignal present in the inflammation environment and subsequently release a ROS scavenger for auto-regulation of ROS balance.					
36067567	3	54	theme	endothelial	797:807	arg1	VEGF					824:827	VEGF	824:827	VEGF	824:827	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	3	54	theme	endothelial	797:807	arg1	factor					816:821	vascular endothelial growth factor	788:821	vascular endothelial growth factor (VEGF)	788:828	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	8	55	theme	feasible	1911:1918	arg1	approach					1920:1927	a feasible approach	1909:1927	a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants	1909:2063	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	8	55	theme	feasible	1911:1918	arg1	incorporation					1765:1777	the incorporation	1761:1777	the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos	1761:1904	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	4	56	dep	showed	909:914	arg1	degraded					980:987	degraded	980:987	degraded the hydrogel	980:1000	Matrix metalloproteinase (MMP), which reportedly showed elevated expression in inflammation response to foreign implant degraded the hydrogel and led to the release of heparin mimicking polymer and VEGF, enhancing its anti-coagulation capacity and accelerating the growth of endothelial cells on it.					
36067567	3	57	theme	MMP-responsive	649:662	arg1	heparin					684:690	MMP-responsive segment-crosslinked heparin	649:690	MMP-responsive segment-crosslinked heparin mimicking polymer	649:708	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	4	58	theme	foreign	964:970	arg1	implant					972:978	foreign implant	964:978	foreign implant	964:978	Matrix metalloproteinase (MMP), which reportedly showed elevated expression in inflammation response to foreign implant degraded the hydrogel and led to the release of heparin mimicking polymer and VEGF, enhancing its anti-coagulation capacity and accelerating the growth of endothelial cells on it.					
36067567	7	59	theme	enhanced	1553:1560	arg1	effects					1603:1609	The enhanced hemocompatibility and endothelialization effects	1549:1609	The enhanced hemocompatibility and endothelialization effects in vivo	1549:1617	The enhanced hemocompatibility and endothelialization effects in vivo were further confirmed by the endovascular implantation of tissues via membrane-covered stent delivery.					
36067567	6	60	theme	subcutaneous	1469:1480	arg1	implantation					1482:1493	subcutaneous implantation	1469:1493	subcutaneous implantation	1469:1493	Subcutaneous implantation in mice suggested that the composite could steer the immune response toward an anti-inflammation state and subcutaneous implantation in rats suggested an anti-calcification effect of it.					
36067567	7	61	dep	in	1611:1612	arg1	vivo					1614:1617	vivo	1614:1617	vivo	1614:1617	The enhanced hemocompatibility and endothelialization effects in vivo were further confirmed by the endovascular implantation of tissues via membrane-covered stent delivery.					
36067567	8	62	theme	anti-calcification	2000:2017	arg1	functions					2019:2027	the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions	1938:2027	the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants	1938:2063	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	2	63	theme	reactive	581:588	arg1	ROS					606:608	ROS	606:608	ROS	606:608	Herein, we report a hydrogel-tissue composite exhibiting inflammation instructive release of multiple components towards preventing coagulation, promoting endothelial growth, and modulating reactive oxygen species (ROS) homeostasis.					
36067567	2	63	theme	reactive	581:588	arg1	species					597:603	reactive oxygen species	581:603	reactive oxygen species (ROS) homeostasis	581:621	Herein, we report a hydrogel-tissue composite exhibiting inflammation instructive release of multiple components towards preventing coagulation, promoting endothelial growth, and modulating reactive oxygen species (ROS) homeostasis.					
36067567	5	64	from	sense	1193:1197	arg1	addition					1163:1170	addition	1163:1170	addition	1163:1170	In addition, the composite could sense oxidation biosignal present in the inflammation environment and subsequently release a ROS scavenger for auto-regulation of ROS balance.					
36067567	5	65	theme	inflammation	1234:1245	arg1	environment					1247:1257	the inflammation environment	1230:1257	the inflammation environment	1230:1257	In addition, the composite could sense oxidation biosignal present in the inflammation environment and subsequently release a ROS scavenger for auto-regulation of ROS balance.					
36067567	3	66	link	segment-crosslinked	664:682	arg1	heparin					684:690	MMP-responsive segment-crosslinked heparin	649:690	MMP-responsive segment-crosslinked heparin mimicking polymer	649:708	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	6	67	theme	anti-calcification	1516:1533	arg1	effect					1535:1540	an anti-calcification effect	1513:1540	an anti-calcification effect of it	1513:1546	Subcutaneous implantation in mice suggested that the composite could steer the immune response toward an anti-inflammation state and subcutaneous implantation in rats suggested an anti-calcification effect of it.					
36067567	8	68	theme	cardiovascular	2041:2054	arg1	implants					2056:2063	HT-based cardiovascular implants	2032:2063	HT-based cardiovascular implants	2032:2063	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	0	69	theme	cardiovascular	127:140	arg1	composite					114:122	hydrogel-tissue composite	98:122	hydrogel-tissue composite	98:122	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin mimicking hydrogel-tissue composite as cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification.					
36067567	0	69	theme	cardiovascular	127:140	arg1	implants					142:149	cardiovascular implants	127:149	cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification	127:233	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin mimicking hydrogel-tissue composite as cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification.					
36067567	2	70	theme	species	597:603	arg1	homeostasis					611:621	reactive oxygen species (ROS) homeostasis	581:621	reactive oxygen species (ROS) homeostasis	581:621	Herein, we report a hydrogel-tissue composite exhibiting inflammation instructive release of multiple components towards preventing coagulation, promoting endothelial growth, and modulating reactive oxygen species (ROS) homeostasis.					
36067567	8	71	theme	current	1727:1733	arg1	findings					1735:1742	The current findings	1723:1742	The current findings	1723:1742	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	2	72	theme	inflammation	448:459	arg1	release					473:479	inflammation instructive release	448:479	inflammation instructive release of multiple components	448:502	Herein, we report a hydrogel-tissue composite exhibiting inflammation instructive release of multiple components towards preventing coagulation, promoting endothelial growth, and modulating reactive oxygen species (ROS) homeostasis.					
36067567	0	73	theme	dual	23:26	arg1	release					28:34	Inflammation-triggered dual release	0:34	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin	0:86	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin mimicking hydrogel-tissue composite as cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification.					
36067567	4	74	theme	endothelial	1135:1145	arg1	cells					1147:1151	endothelial cells	1135:1151	endothelial cells	1135:1151	Matrix metalloproteinase (MMP), which reportedly showed elevated expression in inflammation response to foreign implant degraded the hydrogel and led to the release of heparin mimicking polymer and VEGF, enhancing its anti-coagulation capacity and accelerating the growth of endothelial cells on it.					
36067567	3	75	theme	ROS	750:752	arg1	bonds					778:782	ROS cleavable boronic ester bonds	750:782	ROS cleavable boronic ester bonds	750:782	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	2	76	theme	multiple	484:491	arg1	components					493:502	multiple components	484:502	multiple components	484:502	Herein, we report a hydrogel-tissue composite exhibiting inflammation instructive release of multiple components towards preventing coagulation, promoting endothelial growth, and modulating reactive oxygen species (ROS) homeostasis.					
36067567	5	77	from	environment	1247:1257	arg1	present					1219:1225	present	1219:1225	present	1219:1225	In addition, the composite could sense oxidation biosignal present in the inflammation environment and subsequently release a ROS scavenger for auto-regulation of ROS balance.					
36067567	6	78	from	implantation	1482:1493	arg1	rats					1498:1501	rats	1498:1501	rats suggested an anti-calcification effect of it	1498:1546	Subcutaneous implantation in mice suggested that the composite could steer the immune response toward an anti-inflammation state and subcutaneous implantation in rats suggested an anti-calcification effect of it.					
36067567	4	79	theme	elevated	916:923	arg1	expression					925:934	elevated expression	916:934	elevated expression in inflammation response to foreign implant	916:978	Matrix metalloproteinase (MMP), which reportedly showed elevated expression in inflammation response to foreign implant degraded the hydrogel and led to the release of heparin mimicking polymer and VEGF, enhancing its anti-coagulation capacity and accelerating the growth of endothelial cells on it.					
36067567	0	80	theme	growth	61:66	arg1	factor					68:73	growth factor	61:73	growth factor	61:73	Inflammation-triggered dual release of nitroxide radical and growth factor from heparin mimicking hydrogel-tissue composite as cardiovascular implants for anti-coagulation, endothelialization, anti-inflammation, and anti-calcification.					
36067567	4	81	from	growth	1125:1130	arg1	it					1156:1157	it	1156:1157	it	1156:1157	Matrix metalloproteinase (MMP), which reportedly showed elevated expression in inflammation response to foreign implant degraded the hydrogel and led to the release of heparin mimicking polymer and VEGF, enhancing its anti-coagulation capacity and accelerating the growth of endothelial cells on it.					
36067567	8	82	theme	hydrogel	1793:1800	arg1	approach					1920:1927	a feasible approach	1909:1927	a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants	1909:2063	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	8	82	theme	hydrogel	1793:1800	arg1	incorporation					1765:1777	the incorporation	1761:1777	the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos	1761:1904	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	5	83	theme	ROS	1286:1288	arg1	scavenger					1290:1298	a ROS scavenger	1284:1298	a ROS scavenger for auto-regulation of ROS balance	1284:1333	In addition, the composite could sense oxidation biosignal present in the inflammation environment and subsequently release a ROS scavenger for auto-regulation of ROS balance.					
36067567	7	84	theme	stent	1707:1711	arg1	delivery					1713:1720	membrane-covered stent delivery	1690:1720	membrane-covered stent delivery	1690:1720	The enhanced hemocompatibility and endothelialization effects in vivo were further confirmed by the endovascular implantation of tissues via membrane-covered stent delivery.					
36067567	3	85	theme	boronic	764:770	arg1	bonds					778:782	ROS cleavable boronic ester bonds	750:782	ROS cleavable boronic ester bonds	750:782	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	8	86	theme	multiple	1883:1890	arg1	cargos					1899:1904	multiple active cargos	1883:1904	multiple active cargos	1883:1904	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
36067567	3	87	theme	growth	809:814	arg1	VEGF					824:827	VEGF	824:827	VEGF	824:827	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	3	87	theme	growth	809:814	arg1	factor					816:821	vascular endothelial growth factor	788:821	vascular endothelial growth factor (VEGF)	788:828	The hydrogel composed of MMP-responsive segment-crosslinked heparin mimicking polymer was loaded with a nitroxide radical via ROS cleavable boronic ester bonds and vascular endothelial growth factor (VEGF) via electrostatic attraction.					
36067567	5	88	from	present	1219:1225	arg1	environment					1247:1257	the inflammation environment	1230:1257	the inflammation environment	1230:1257	In addition, the composite could sense oxidation biosignal present in the inflammation environment and subsequently release a ROS scavenger for auto-regulation of ROS balance.					
36067567	8	89	theme	cargos	1899:1904	arg1	release					1872:1878	controlled release	1861:1878	controlled release of multiple active cargos	1861:1904	The current findings demonstrate that the incorporation of functional hydrogel into the tissue sophistically exploiting host response for controlled release of multiple active cargos is a feasible approach to boost the anticoagulant, endothelialization, anti-inflammatory, and anti-calcification functions of HT-based cardiovascular implants.					
35218809	0	0	theme	drug-loading	120:131	arg1	microspheres					133:144	antibacterial drug-loading microspheres	106:144	antibacterial drug-loading microspheres for wound dressing materials	106:173	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film loaded with antibacterial drug-loading microspheres for wound dressing materials.					
35218809	0	1	theme	antibacterial	106:118	arg1	microspheres					133:144	antibacterial drug-loading microspheres	106:144	antibacterial drug-loading microspheres for wound dressing materials	106:173	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film loaded with antibacterial drug-loading microspheres for wound dressing materials.					
35218809	8	2	theme	biomaterials	1522:1533	arg1	use					1490:1492	the use	1486:1492	the use of additional wound healing biomaterials in clinical medicine	1486:1554	This study could stimulate and promote the use of additional wound healing biomaterials in clinical medicine.					
35218809	1	3	theme	wound	213:217	arg1	infection					219:227	wound infection	213:227	wound infection	213:227	Drug delivery technology can prevent wound infection and inflammatory reactions and accelerate wound healing and quality.					
35218809	7	4	theme	wound	1229:1233	arg1	exudate					1235:1241	excess wound exudate	1222:1241	excess wound exudate	1222:1241	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	3	5	theme	multi-layered	421:433	arg1	membrane					455:462	A multi-layered composite nanofiber membrane	419:462	A multi-layered composite nanofiber membrane	419:462	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	2	6	theme	people	398:403	arg1	various					381:387	various	381:387	various	381:387	In this paper, we propose preparing a multifunctional medical dressing to meet the various needs of people for dressing.					
35218809	5	7	theme	cumulative	1059:1068	arg1	%					1101:1101	54.46%	1096:1101	54.46%	1096:1101	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	5	7	theme	cumulative	1059:1068	arg1	rate					1078:1081	the cumulative release rate	1055:1081	the cumulative release rate in vitro	1055:1090	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	2	8	theme	multifunctional	336:350	arg1	dressing					360:367	a multifunctional medical dressing	334:367	a multifunctional medical dressing to meet the various needs of people for dressing	334:416	In this paper, we propose preparing a multifunctional medical dressing to meet the various needs of people for dressing.					
35218809	6	9	theme	good	1156:1159	arg1	effect					1175:1180	its good antibacterial effect	1152:1180	its good antibacterial effect	1152:1180	The results of in vitro experiments also showed its good antibacterial effect and good biocompatibility.					
35218809	5	10	theme	serum	982:986	arg1	BSA					997:999	BSA	997:999	BSA	997:999	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	5	10	theme	serum	982:986	arg1	albumin					988:994	bovine serum albumin	975:994	bovine serum albumin (BSA)	975:1000	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	7	11	theme	cumulative	1272:1281	arg1	AOTC					1318:1321	AOTC	1318:1321	AOTC	1318:1321	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	7	11	theme	cumulative	1272:1281	arg1	capacity					1308:1315	the cumulative unidirectional transport capacity	1268:1315	the cumulative unidirectional transport capacity (AOTC) of 651.75%	1268:1333	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	7	12	from	microspheres	1364:1375	arg1	membrane					1437:1444	an SF/PCL- PVA unidirectional water conductive composite membrane	1380:1444	an SF/PCL- PVA unidirectional water conductive composite membrane	1380:1444	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	5	13	theme	bovine	975:980	arg1	BSA					997:999	BSA	997:999	BSA	997:999	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	5	13	theme	bovine	975:980	arg1	albumin					988:994	bovine serum albumin	975:994	bovine serum albumin (BSA)	975:1000	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	0	14	theme	dressing	156:163	arg1	materials					165:173	wound dressing materials	150:173	wound dressing materials	150:173	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film loaded with antibacterial drug-loading microspheres for wound dressing materials.					
35218809	4	15	theme	water-conducting	873:888	arg1	membrane					910:917	the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane	789:917	the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane	789:917	Then the MSN-SF/CS microspheres were sprayed on the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane.					
35218809	5	16	theme	MSN-SF/CS	1005:1013	arg1	microspheres					1027:1038	MSN-SF/CS drug-loaded microspheres	1005:1038	MSN-SF/CS drug-loaded microspheres	1005:1038	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	3	17	theme	silk	486:489	arg1	fibroin					491:497	silk fibroin	486:497	silk fibroin	486:497	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	3	17	theme	silk	486:489	arg1	substrate					506:514	the substrate	502:514	the substrate	502:514	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	0	18	theme	wound	150:154	arg1	materials					165:173	wound dressing materials	150:173	wound dressing materials	150:173	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film loaded with antibacterial drug-loading microspheres for wound dressing materials.					
35218809	3	19	with	nanoparticles	539:551	arg1	properties					580:589	high adsorption properties	564:589	high adsorption properties	564:589	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	3	20	theme	nanofiber	445:453	arg1	membrane					455:462	A multi-layered composite nanofiber membrane	419:462	A multi-layered composite nanofiber membrane	419:462	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	8	21	theme	additional	1497:1506	arg1	biomaterials					1522:1533	additional wound healing biomaterials	1497:1533	additional wound healing biomaterials	1497:1533	This study could stimulate and promote the use of additional wound healing biomaterials in clinical medicine.					
35218809	0	22	theme	fibroin/polycaprolactone-polyvinyl	5:38	arg1	film					89:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film loaded with antibacterial drug-loading microspheres for wound dressing materials.					
35218809	6	23	dep	in	1119:1120	arg1	vitro					1122:1126	vitro	1122:1126	vitro	1122:1126	The results of in vitro experiments also showed its good antibacterial effect and good biocompatibility.					
35218809	4	24	theme	nanofiber	900:908	arg1	membrane					910:917	the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane	789:917	the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane	789:917	Then the MSN-SF/CS microspheres were sprayed on the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane.					
35218809	2	25	dep	various	381:387	arg1	needs					389:393	needs	389:393	needs	389:393	In this paper, we propose preparing a multifunctional medical dressing to meet the various needs of people for dressing.					
35218809	1	26	theme	inflammatory	233:244	arg1	reactions					246:254	inflammatory reactions	233:254	inflammatory reactions	233:254	Drug delivery technology can prevent wound infection and inflammatory reactions and accelerate wound healing and quality.					
35218809	0	27	theme	Silk	0:3	arg1	film					89:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film loaded with antibacterial drug-loading microspheres for wound dressing materials.					
35218809	4	28	theme	composite	890:898	arg1	membrane					910:917	the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane	789:917	the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane	789:917	Then the MSN-SF/CS microspheres were sprayed on the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane.					
35218809	5	29	theme	albumin	988:994	arg1	rate					967:970	the encapsulation rate	949:970	the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres	949:1038	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	5	29	theme	albumin	988:994	arg1	%					1049:1049	65.53%	1044:1049	65.53%	1044:1049	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	5	30	theme	test	924:927	arg1	results					929:935	The test results	920:935	The test results	920:935	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	0	31	theme	directional	48:58	arg1	film					89:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film loaded with antibacterial drug-loading microspheres for wound dressing materials.					
35218809	5	32	theme	release	1070:1076	arg1	%					1101:1101	54.46%	1096:1101	54.46%	1096:1101	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	5	32	theme	release	1070:1076	arg1	rate					1078:1081	the cumulative release rate	1055:1081	the cumulative release rate in vitro	1055:1090	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	6	33	theme	antibacterial	1161:1173	arg1	effect					1175:1180	its good antibacterial effect	1152:1180	its good antibacterial effect	1152:1180	The results of in vitro experiments also showed its good antibacterial effect and good biocompatibility.					
35218809	7	34	theme	%	1333:1333	arg1	AOTC					1318:1321	AOTC	1318:1321	AOTC	1318:1321	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	7	34	theme	%	1333:1333	arg1	capacity					1308:1315	the cumulative unidirectional transport capacity	1268:1315	the cumulative unidirectional transport capacity (AOTC) of 651.75%	1268:1333	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	0	35	theme	alcohol	40:46	arg1	film					89:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film loaded with antibacterial drug-loading microspheres for wound dressing materials.					
35218809	8	36	theme	healing	1514:1520	arg1	biomaterials					1522:1533	additional wound healing biomaterials	1497:1533	additional wound healing biomaterials	1497:1533	This study could stimulate and promote the use of additional wound healing biomaterials in clinical medicine.					
35218809	2	37	theme	medical	352:358	arg1	dressing					360:367	a multifunctional medical dressing	334:367	a multifunctional medical dressing to meet the various needs of people for dressing	334:416	In this paper, we propose preparing a multifunctional medical dressing to meet the various needs of people for dressing.					
35218809	3	38	theme	mesoporous	521:530	arg1	MSN					554:556	MSN	554:556	MSN	554:556	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	3	38	theme	mesoporous	521:530	arg1	nanoparticles					539:551	mesoporous silica nanoparticles	521:551	mesoporous silica nanoparticles (MSN) with high adsorption properties	521:589	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	0	39	theme	transport	69:77	arg1	film					89:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film loaded with antibacterial drug-loading microspheres for wound dressing materials.					
35218809	7	40	theme	unidirectional	1283:1296	arg1	AOTC					1318:1321	AOTC	1318:1321	AOTC	1318:1321	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	7	40	theme	unidirectional	1283:1296	arg1	capacity					1308:1315	the cumulative unidirectional transport capacity	1268:1315	the cumulative unidirectional transport capacity (AOTC) of 651.75%	1268:1333	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	1	41	theme	wound	271:275	arg1	healing					277:283	wound healing	271:283	wound healing	271:283	Drug delivery technology can prevent wound infection and inflammatory reactions and accelerate wound healing and quality.					
35218809	0	42	theme	moisture	60:67	arg1	film					89:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film loaded with antibacterial drug-loading microspheres for wound dressing materials.					
35218809	7	43	theme	composite	1427:1435	arg1	membrane					1437:1444	an SF/PCL- PVA unidirectional water conductive composite membrane	1380:1444	an SF/PCL- PVA unidirectional water conductive composite membrane	1380:1444	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	3	44	theme	SF	652:653	arg1	/chitosan					655:663	silk fibroin (SF)/chitosan	638:663	silk fibroin (SF)/chitosan (CS) microspheres	638:681	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	3	44	theme	SF	652:653	arg1	CS					666:667	CS	666:667	CS	666:667	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	7	45	theme	excess	1222:1227	arg1	exudate					1235:1241	excess wound exudate	1222:1241	excess wound exudate	1222:1241	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	3	46	theme	silk	638:641	arg1	/chitosan					655:663	silk fibroin (SF)/chitosan	638:663	silk fibroin (SF)/chitosan (CS) microspheres	638:681	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	3	46	theme	silk	638:641	arg1	CS					666:667	CS	666:667	CS	666:667	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	5	47	theme	encapsulation	953:965	arg1	rate					967:970	the encapsulation rate	949:970	the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres	949:1038	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	5	47	theme	encapsulation	953:965	arg1	%					1049:1049	65.53%	1044:1049	65.53%	1044:1049	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	0	48	theme	composite	79:87	arg1	film					89:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film	0:92	Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film loaded with antibacterial drug-loading microspheres for wound dressing materials.					
35218809	4	49	theme	unidirectional	858:871	arg1	membrane					910:917	the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane	789:917	the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane	789:917	Then the MSN-SF/CS microspheres were sprayed on the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane.					
35218809	8	50	theme	wound	1508:1512	arg1	biomaterials					1522:1533	additional wound healing biomaterials	1497:1533	additional wound healing biomaterials	1497:1533	This study could stimulate and promote the use of additional wound healing biomaterials in clinical medicine.					
35218809	3	51	theme	fibroin	643:649	arg1	/chitosan					655:663	silk fibroin (SF)/chitosan	638:663	silk fibroin (SF)/chitosan (CS) microspheres	638:681	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	3	51	theme	fibroin	643:649	arg1	CS					666:667	CS	666:667	CS	666:667	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	4	52	theme	MSN-SF/CS	750:758	arg1	microspheres					760:771	Then the MSN-SF/CS microspheres	741:771	Then the MSN-SF/CS microspheres	741:771	Then the MSN-SF/CS microspheres were sprayed on the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane.					
35218809	7	53	theme	transport	1298:1306	arg1	AOTC					1318:1321	AOTC	1318:1321	AOTC	1318:1321	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	7	53	theme	transport	1298:1306	arg1	capacity					1308:1315	the cumulative unidirectional transport capacity	1268:1315	the cumulative unidirectional transport capacity (AOTC) of 651.75%	1268:1333	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	3	54	theme	uniform	719:725	arg1	distribution					727:738	uniform distribution	719:738	uniform distribution	719:738	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	7	55	theme	conductive	1416:1425	arg1	membrane					1437:1444	an SF/PCL- PVA unidirectional water conductive composite membrane	1380:1444	an SF/PCL- PVA unidirectional water conductive composite membrane	1380:1444	To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane.					
35218809	1	56	theme	Drug	176:179	arg1	technology					190:199	Drug delivery technology	176:199	Drug delivery technology	176:199	Drug delivery technology can prevent wound infection and inflammatory reactions and accelerate wound healing and quality.					
35218809	6	57	theme	experiments	1128:1138	arg1	results					1108:1114	The results	1104:1114	The results of in vitro experiments	1104:1138	The results of in vitro experiments also showed its good antibacterial effect and good biocompatibility.					
35218809	5	58	theme	drug-loaded	1015:1025	arg1	microspheres					1027:1038	MSN-SF/CS drug-loaded microspheres	1005:1038	MSN-SF/CS drug-loaded microspheres	1005:1038	The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%.					
35218809	3	59	theme	MSN-SF/CS	691:699	arg1	microspheres					701:712	MSN-SF/CS microspheres	691:712	MSN-SF/CS microspheres	691:712	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	1	60	theme	delivery	181:188	arg1	technology					190:199	Drug delivery technology	176:199	Drug delivery technology	176:199	Drug delivery technology can prevent wound infection and inflammatory reactions and accelerate wound healing and quality.					
35218809	3	61	theme	silica	532:537	arg1	MSN					554:556	MSN	554:556	MSN	554:556	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	3	61	theme	silica	532:537	arg1	nanoparticles					539:551	mesoporous silica nanoparticles	521:551	mesoporous silica nanoparticles (MSN) with high adsorption properties	521:589	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	6	62	theme	in	1119:1120	arg1	experiments					1128:1138	in vitro experiments	1119:1138	in vitro experiments	1119:1138	The results of in vitro experiments also showed its good antibacterial effect and good biocompatibility.					
35218809	6	63	theme	good	1186:1189	arg1	biocompatibility					1191:1206	good biocompatibility	1186:1206	good biocompatibility	1186:1206	The results of in vitro experiments also showed its good antibacterial effect and good biocompatibility.					
35218809	3	64	theme	high	564:567	arg1	properties					580:589	high adsorption properties	564:589	high adsorption properties	564:589	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	3	65	theme	composite	435:443	arg1	membrane					455:462	A multi-layered composite nanofiber membrane	419:462	A multi-layered composite nanofiber membrane	419:462	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	8	66	from	use	1490:1492	arg1	medicine					1547:1554	clinical medicine	1538:1554	clinical medicine	1538:1554	This study could stimulate and promote the use of additional wound healing biomaterials in clinical medicine.					
35218809	8	67	theme	clinical	1538:1545	arg1	medicine					1547:1554	clinical medicine	1538:1554	clinical medicine	1538:1554	This study could stimulate and promote the use of additional wound healing biomaterials in clinical medicine.					
35218809	3	68	theme	/chitosan	655:663	arg1	microspheres					670:681	silk fibroin (SF)/chitosan (CS) microspheres	638:681	silk fibroin (SF)/chitosan (CS) microspheres	638:681	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
35218809	3	69	theme	adsorption	569:578	arg1	properties					580:589	high adsorption properties	564:589	high adsorption properties	564:589	A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution.					
36958442	0	0	theme	hemostasis	115:124	arg1	dressing					126:133	high-performance hemostasis dressing	98:133	high-performance hemostasis dressing	98:133	Engineering design of asymmetric halloysite/chitosan/collagen sponge with hydrophobic coating for high-performance hemostasis dressing.					
36958442	4	1	theme	electrostatic	752:764	arg1	attractions					766:776	electrostatic attractions	752:776	electrostatic attractions	752:776	Morphology and spectrum results suggested that chitosan and collagen are capable of adsorbing on the outer surface of HNTs due to the hydrogen bonding and electrostatic attractions.					
36958442	9	2	theme	dressing	1422:1429	arg1	technique					1465:1473	a high-performance and hemostatic dressing by material design and processing technique	1388:1473	a high-performance and hemostatic dressing by material design and processing technique	1388:1473	This work developed a high-performance and hemostatic dressing by material design and processing technique, which shows a promising application in wound healing.					
36958442	1	3	theme	death	190:194	arg1	hemorrhage					157:166	Uncontrolled massive hemorrhage	136:166	Uncontrolled massive hemorrhage	136:166	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	1	3	theme	death	190:194	arg1	importance					264:273	great medical importance	250:273	great medical importance	250:273	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	1	3	theme	death	190:194	arg1	cause					181:185	a crucial cause	171:185	a crucial cause of death	171:194	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	0	4	theme	high-performance	98:113	arg1	dressing					126:133	high-performance hemostasis dressing	98:133	high-performance hemostasis dressing	98:133	Engineering design of asymmetric halloysite/chitosan/collagen sponge with hydrophobic coating for high-performance hemostasis dressing.					
36958442	7	5	theme	blood	1221:1225	arg1	absorption					1227:1236	excessive blood absorption	1211:1236	excessive blood absorption	1211:1236	The hemostatic dressing avoided unnecessary blood loss because of excessive blood absorption.					
36958442	7	6	theme	unnecessary	1177:1187	arg1	loss					1195:1198	unnecessary blood loss	1177:1198	unnecessary blood loss	1177:1198	The hemostatic dressing avoided unnecessary blood loss because of excessive blood absorption.					
36958442	2	7	theme	hydrophobic	406:416	arg1	coating					439:445	hydrophobic polydimethylsiloxane coating	406:445	hydrophobic polydimethylsiloxane coating	406:445	Herein, we prepared a halloysite-chitosan-collagen composite sponge by directional freeze-drying method and coating the sponge by hydrophobic polydimethylsiloxane coating for rapid and effective hemostasis.					
36958442	7	8	theme	excessive	1211:1219	arg1	absorption					1227:1236	excessive blood absorption	1211:1236	excessive blood absorption	1211:1236	The hemostatic dressing avoided unnecessary blood loss because of excessive blood absorption.					
36958442	5	9	theme	freeze-dried	795:806	arg1	sponge					808:813	The directional freeze-dried sponge	779:813	The directional freeze-dried sponge	779:813	The directional freeze-dried sponge absorbed the majority of the blood within 10 s, and that process essentially completed in 30 s, which are faster than its non-directional counterpart.					
36958442	6	10	theme	composite	970:978	arg1	sponges					980:986	The composite sponges	966:986	The composite sponges	966:986	The composite sponges exhibited high antibacterial properties towards E. coli and S. aureus, and they are non-cytotoxic towards mouse fibroblasts and have high hemocompatibility.					
36958442	6	11	theme	antibacterial	1003:1015	arg1	properties					1017:1026	high antibacterial properties	998:1026	high antibacterial properties towards E. coli and S. aureus	998:1056	The composite sponges exhibited high antibacterial properties towards E. coli and S. aureus, and they are non-cytotoxic towards mouse fibroblasts and have high hemocompatibility.					
36958442	1	12	theme	massive	149:155	arg1	hemorrhage					157:166	Uncontrolled massive hemorrhage	136:166	Uncontrolled massive hemorrhage	136:166	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	1	12	theme	massive	149:155	arg1	cause					181:185	a crucial cause	171:185	a crucial cause of death	171:194	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	9	13	theme	high-performance	1390:1405	arg1	technique					1465:1473	a high-performance and hemostatic dressing by material design and processing technique	1388:1473	a high-performance and hemostatic dressing by material design and processing technique	1388:1473	This work developed a high-performance and hemostatic dressing by material design and processing technique, which shows a promising application in wound healing.					
36958442	2	14	theme	polydimethylsiloxane	418:437	arg1	coating					439:445	hydrophobic polydimethylsiloxane coating	406:445	hydrophobic polydimethylsiloxane coating	406:445	Herein, we prepared a halloysite-chitosan-collagen composite sponge by directional freeze-drying method and coating the sponge by hydrophobic polydimethylsiloxane coating for rapid and effective hemostasis.					
36958442	3	15	theme	~30 μm	547:552	arg1	size					539:542	a pore size	532:542	a pore size of ~30 μm	532:552	The aligned channel structure of the sponge with a pore size of ~30 μm was beneficial for the transport of blood.					
36958442	6	16	contain	have	1116:1119	arg1	they					1063:1066	they	1063:1066	they	1063:1066	The composite sponges exhibited high antibacterial properties towards E. coli and S. aureus, and they are non-cytotoxic towards mouse fibroblasts and have high hemocompatibility.					
36958442	6	16	contain	have	1116:1119	arg2	hemocompatibility					1126:1142	high hemocompatibility	1121:1142	high hemocompatibility	1121:1142	The composite sponges exhibited high antibacterial properties towards E. coli and S. aureus, and they are non-cytotoxic towards mouse fibroblasts and have high hemocompatibility.					
36958442	7	17	theme	blood	1189:1193	arg1	loss					1195:1198	unnecessary blood loss	1177:1198	unnecessary blood loss	1177:1198	The hemostatic dressing avoided unnecessary blood loss because of excessive blood absorption.					
36958442	8	18	theme	reducing	1347:1354	arg1	loss					1362:1365	reducing blood loss	1347:1365	reducing blood loss	1347:1365	In vivo experiments of rats also confirmed the ability of the asymmetric sponges to rapidly clot and reduce reducing blood loss.					
36958442	2	19	theme	composite	327:335	arg1	sponge					337:342	a halloysite-chitosan-collagen composite sponge	296:342	a halloysite-chitosan-collagen composite sponge	296:342	Herein, we prepared a halloysite-chitosan-collagen composite sponge by directional freeze-drying method and coating the sponge by hydrophobic polydimethylsiloxane coating for rapid and effective hemostasis.					
36958442	8	20	theme	sponges	1312:1318	arg1	ability					1286:1292	the ability	1282:1292	the ability of the asymmetric sponges to rapidly clot and reduce reducing blood loss	1282:1365	In vivo experiments of rats also confirmed the ability of the asymmetric sponges to rapidly clot and reduce reducing blood loss.					
36958442	9	21	theme	hemostatic	1411:1420	arg1	dressing					1422:1429	hemostatic dressing	1411:1429	hemostatic dressing by material design	1411:1448	This work developed a high-performance and hemostatic dressing by material design and processing technique, which shows a promising application in wound healing.					
36958442	3	22	theme	aligned	487:493	arg1	structure					503:511	The aligned channel structure	483:511	The aligned channel structure of the sponge with a pore size of ~30 μm	483:552	The aligned channel structure of the sponge with a pore size of ~30 μm was beneficial for the transport of blood.					
36958442	3	22	theme	aligned	487:493	arg1	beneficial					558:567	beneficial	558:567	beneficial	558:567	The aligned channel structure of the sponge with a pore size of ~30 μm was beneficial for the transport of blood.					
36958442	2	23	theme	halloysite-chitosan-collagen	298:325	arg1	sponge					337:342	a halloysite-chitosan-collagen composite sponge	296:342	a halloysite-chitosan-collagen composite sponge	296:342	Herein, we prepared a halloysite-chitosan-collagen composite sponge by directional freeze-drying method and coating the sponge by hydrophobic polydimethylsiloxane coating for rapid and effective hemostasis.					
36958442	1	24	theme	developing	201:210	arg1	materials					233:241	developing efficient hemostatic materials	201:241	developing efficient hemostatic materials	201:241	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	5	25	theme	blood	844:848	arg1	majority					828:835	the majority	824:835	the majority of the blood	824:848	The directional freeze-dried sponge absorbed the majority of the blood within 10 s, and that process essentially completed in 30 s, which are faster than its non-directional counterpart.					
36958442	4	26	theme	HNTs	715:718	arg1	surface					704:710	the outer surface	694:710	the outer surface of HNTs	694:718	Morphology and spectrum results suggested that chitosan and collagen are capable of adsorbing on the outer surface of HNTs due to the hydrogen bonding and electrostatic attractions.					
36958442	1	27	theme	efficient	212:220	arg1	materials					233:241	developing efficient hemostatic materials	201:241	developing efficient hemostatic materials	201:241	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	8	28	theme	rats	1262:1265	arg1	experiments					1247:1257	In vivo experiments	1239:1257	In vivo experiments of rats	1239:1265	In vivo experiments of rats also confirmed the ability of the asymmetric sponges to rapidly clot and reduce reducing blood loss.					
36958442	4	29	dep	bonding	740:746	arg1	the					727:729	the	727:729	the	727:729	Morphology and spectrum results suggested that chitosan and collagen are capable of adsorbing on the outer surface of HNTs due to the hydrogen bonding and electrostatic attractions.					
36958442	2	30	theme	rapid	451:455	arg1	hemostasis					471:480	rapid and effective hemostasis	451:480	rapid and effective hemostasis	451:480	Herein, we prepared a halloysite-chitosan-collagen composite sponge by directional freeze-drying method and coating the sponge by hydrophobic polydimethylsiloxane coating for rapid and effective hemostasis.					
36958442	0	31	theme	asymmetric	22:31	arg1	sponge					62:67	asymmetric halloysite/chitosan/collagen sponge	22:67	asymmetric halloysite/chitosan/collagen sponge	22:67	Engineering design of asymmetric halloysite/chitosan/collagen sponge with hydrophobic coating for high-performance hemostasis dressing.					
36958442	4	32	theme	spectrum	612:619	arg1	results					621:627	Morphology and spectrum results	597:627	Morphology and spectrum results	597:627	Morphology and spectrum results suggested that chitosan and collagen are capable of adsorbing on the outer surface of HNTs due to the hydrogen bonding and electrostatic attractions.					
36958442	7	33	theme	hemostatic	1149:1158	arg1	dressing					1160:1167	The hemostatic dressing	1145:1167	The hemostatic dressing	1145:1167	The hemostatic dressing avoided unnecessary blood loss because of excessive blood absorption.					
36958442	1	34	theme	Uncontrolled	136:147	arg1	hemorrhage					157:166	Uncontrolled massive hemorrhage	136:166	Uncontrolled massive hemorrhage	136:166	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	1	34	theme	Uncontrolled	136:147	arg1	cause					181:185	a crucial cause	171:185	a crucial cause of death	171:194	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	3	35	theme	pore	534:537	arg1	size					539:542	a pore size	532:542	a pore size of ~30 μm	532:552	The aligned channel structure of the sponge with a pore size of ~30 μm was beneficial for the transport of blood.					
36958442	3	36	theme	blood	590:594	arg1	transport					577:585	the transport	573:585	the transport of blood	573:594	The aligned channel structure of the sponge with a pore size of ~30 μm was beneficial for the transport of blood.					
36958442	2	37	theme	directional	347:357	arg1	method					373:378	directional freeze-drying method	347:378	directional freeze-drying method	347:378	Herein, we prepared a halloysite-chitosan-collagen composite sponge by directional freeze-drying method and coating the sponge by hydrophobic polydimethylsiloxane coating for rapid and effective hemostasis.					
36958442	6	38	theme	high	1121:1124	arg1	hemocompatibility					1126:1142	high hemocompatibility	1121:1142	high hemocompatibility	1121:1142	The composite sponges exhibited high antibacterial properties towards E. coli and S. aureus, and they are non-cytotoxic towards mouse fibroblasts and have high hemocompatibility.					
36958442	0	39	theme	sponge	62:67	arg1	design					12:17	design	12:17	design of asymmetric halloysite/chitosan/collagen sponge with hydrophobic coating for high-performance hemostasis dressing	12:133	Engineering design of asymmetric halloysite/chitosan/collagen sponge with hydrophobic coating for high-performance hemostasis dressing.					
36958442	1	40	theme	hemostatic	222:231	arg1	materials					233:241	developing efficient hemostatic materials	201:241	developing efficient hemostatic materials	201:241	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	0	41	theme	halloysite/chitosan/collagen	33:60	arg1	sponge					62:67	asymmetric halloysite/chitosan/collagen sponge	22:67	asymmetric halloysite/chitosan/collagen sponge	22:67	Engineering design of asymmetric halloysite/chitosan/collagen sponge with hydrophobic coating for high-performance hemostasis dressing.					
36958442	9	42	theme	processing	1454:1463	arg1	technique					1465:1473	a high-performance and hemostatic dressing by material design and processing technique	1388:1473	a high-performance and hemostatic dressing by material design and processing technique	1388:1473	This work developed a high-performance and hemostatic dressing by material design and processing technique, which shows a promising application in wound healing.					
36958442	3	43	theme	channel	495:501	arg1	structure					503:511	The aligned channel structure	483:511	The aligned channel structure of the sponge with a pore size of ~30 μm	483:552	The aligned channel structure of the sponge with a pore size of ~30 μm was beneficial for the transport of blood.					
36958442	3	43	theme	channel	495:501	arg1	beneficial					558:567	beneficial	558:567	beneficial	558:567	The aligned channel structure of the sponge with a pore size of ~30 μm was beneficial for the transport of blood.					
36958442	8	44	theme	In	1239:1240	arg1	experiments					1247:1257	In vivo experiments	1239:1257	In vivo experiments of rats	1239:1265	In vivo experiments of rats also confirmed the ability of the asymmetric sponges to rapidly clot and reduce reducing blood loss.					
36958442	1	45	theme	great	250:254	arg1	importance					264:273	great medical importance	250:273	great medical importance	250:273	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	0	46	theme	hydrophobic	74:84	arg1	coating					86:92	hydrophobic coating	74:92	hydrophobic coating for high-performance hemostasis dressing	74:133	Engineering design of asymmetric halloysite/chitosan/collagen sponge with hydrophobic coating for high-performance hemostasis dressing.					
36958442	3	47	with	structure	503:511	arg1	size					539:542	a pore size	532:542	a pore size of ~30 μm	532:552	The aligned channel structure of the sponge with a pore size of ~30 μm was beneficial for the transport of blood.					
36958442	9	48	theme	material	1434:1441	arg1	design					1443:1448	material design	1434:1448	material design	1434:1448	This work developed a high-performance and hemostatic dressing by material design and processing technique, which shows a promising application in wound healing.					
36958442	5	49	theme	directional	783:793	arg1	sponge					808:813	The directional freeze-dried sponge	779:813	The directional freeze-dried sponge	779:813	The directional freeze-dried sponge absorbed the majority of the blood within 10 s, and that process essentially completed in 30 s, which are faster than its non-directional counterpart.					
36958442	9	50	from	application	1500:1510	arg1	healing					1521:1527	wound healing	1515:1527	wound healing	1515:1527	This work developed a high-performance and hemostatic dressing by material design and processing technique, which shows a promising application in wound healing.					
36958442	8	51	dep	In	1239:1240	arg1	vivo					1242:1245	vivo	1242:1245	vivo	1242:1245	In vivo experiments of rats also confirmed the ability of the asymmetric sponges to rapidly clot and reduce reducing blood loss.					
36958442	0	52	with	design	12:17	arg1	coating					86:92	hydrophobic coating	74:92	hydrophobic coating for high-performance hemostasis dressing	74:133	Engineering design of asymmetric halloysite/chitosan/collagen sponge with hydrophobic coating for high-performance hemostasis dressing.					
36958442	8	53	theme	asymmetric	1301:1310	arg1	sponges					1312:1318	the asymmetric sponges	1297:1318	the asymmetric sponges	1297:1318	In vivo experiments of rats also confirmed the ability of the asymmetric sponges to rapidly clot and reduce reducing blood loss.					
36958442	4	54	theme	Morphology	597:606	arg1	results					621:627	Morphology and spectrum results	597:627	Morphology and spectrum results	597:627	Morphology and spectrum results suggested that chitosan and collagen are capable of adsorbing on the outer surface of HNTs due to the hydrogen bonding and electrostatic attractions.					
36958442	6	55	theme	mouse	1094:1098	arg1	fibroblasts					1100:1110	mouse fibroblasts	1094:1110	mouse fibroblasts	1094:1110	The composite sponges exhibited high antibacterial properties towards E. coli and S. aureus, and they are non-cytotoxic towards mouse fibroblasts and have high hemocompatibility.					
36958442	2	56	theme	freeze-drying	359:371	arg1	method					373:378	directional freeze-drying method	347:378	directional freeze-drying method	347:378	Herein, we prepared a halloysite-chitosan-collagen composite sponge by directional freeze-drying method and coating the sponge by hydrophobic polydimethylsiloxane coating for rapid and effective hemostasis.					
36958442	1	57	theme	medical	256:262	arg1	importance					264:273	great medical importance	250:273	great medical importance	250:273	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	9	58	theme	wound	1515:1519	arg1	healing					1521:1527	wound healing	1515:1527	wound healing	1515:1527	This work developed a high-performance and hemostatic dressing by material design and processing technique, which shows a promising application in wound healing.					
36958442	1	59	theme	crucial	173:179	arg1	hemorrhage					157:166	Uncontrolled massive hemorrhage	136:166	Uncontrolled massive hemorrhage	136:166	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	1	59	theme	crucial	173:179	arg1	cause					181:185	a crucial cause	171:185	a crucial cause of death	171:194	Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance.					
36958442	3	60	theme	sponge	520:525	arg1	structure					503:511	The aligned channel structure	483:511	The aligned channel structure of the sponge with a pore size of ~30 μm	483:552	The aligned channel structure of the sponge with a pore size of ~30 μm was beneficial for the transport of blood.					
36958442	3	60	theme	sponge	520:525	arg1	beneficial					558:567	beneficial	558:567	beneficial	558:567	The aligned channel structure of the sponge with a pore size of ~30 μm was beneficial for the transport of blood.					
36958442	4	61	theme	outer	698:702	arg1	surface					704:710	the outer surface	694:710	the outer surface of HNTs	694:718	Morphology and spectrum results suggested that chitosan and collagen are capable of adsorbing on the outer surface of HNTs due to the hydrogen bonding and electrostatic attractions.					
36958442	5	62	theme	non-directional	937:951	arg1	counterpart					953:963	its non-directional counterpart	933:963	its non-directional counterpart	933:963	The directional freeze-dried sponge absorbed the majority of the blood within 10 s, and that process essentially completed in 30 s, which are faster than its non-directional counterpart.					
36958442	2	63	theme	effective	461:469	arg1	hemostasis					471:480	rapid and effective hemostasis	451:480	rapid and effective hemostasis	451:480	Herein, we prepared a halloysite-chitosan-collagen composite sponge by directional freeze-drying method and coating the sponge by hydrophobic polydimethylsiloxane coating for rapid and effective hemostasis.					
36958442	9	64	theme	promising	1490:1498	arg1	application					1500:1510	a promising application	1488:1510	a promising application in wound healing	1488:1527	This work developed a high-performance and hemostatic dressing by material design and processing technique, which shows a promising application in wound healing.					
36958442	6	65	theme	high	998:1001	arg1	properties					1017:1026	high antibacterial properties	998:1026	high antibacterial properties towards E. coli and S. aureus	998:1056	The composite sponges exhibited high antibacterial properties towards E. coli and S. aureus, and they are non-cytotoxic towards mouse fibroblasts and have high hemocompatibility.					
36958442	8	66	theme	blood	1356:1360	arg1	loss					1362:1365	reducing blood loss	1347:1365	reducing blood loss	1347:1365	In vivo experiments of rats also confirmed the ability of the asymmetric sponges to rapidly clot and reduce reducing blood loss.					
36958442	4	67	theme	hydrogen	731:738	arg1	bonding					740:746	hydrogen bonding	731:746	hydrogen bonding	731:746	Morphology and spectrum results suggested that chitosan and collagen are capable of adsorbing on the outer surface of HNTs due to the hydrogen bonding and electrostatic attractions.					
36086517	3	0	attach	derived	468:474	arg2	scaffold					459:466	a 3D printed scaffold	446:466	a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	446:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	3	0	attach	derived	468:474	arg1	bioink					499:504	a novel composite bioink	481:504	a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	481:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	3	1	theme	composite	489:497	arg1	bioink					499:504	a novel composite bioink	481:504	a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	481:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	2	2	from	individual	289:298	arg1	donor					314:318	an outside donor	303:318	an outside donor	303:318	Biological scaffolds require bone extraction from the individual or an outside donor while synthetic grafts mostly suffer from poor degradation kinetics and decreased bioactivity.					
36086517	2	2	from	individual	289:298	arg1	extraction					269:278	bone extraction	264:278	bone extraction from the individual	264:298	Biological scaffolds require bone extraction from the individual or an outside donor while synthetic grafts mostly suffer from poor degradation kinetics and decreased bioactivity.					
36086517	0	3	theme	Tissue	95:100	arg1	Engineering					102:112	Bone Tissue Engineering	90:112	Bone Tissue Engineering	90:112	Controlled Biodegradation and Swelling of Strontium-doped Alginate/Collagen Scaffolds for Bone Tissue Engineering.					
36086517	5	4	theme	long-term	1046:1054	arg1	degradation					1056:1066	adjustable long-term degradation	1035:1066	adjustable long-term degradation	1035:1066	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	3	5	dep	L	598:598	arg1	to					585:586	to	585:586	to	585:586	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	5	6	theme	suitable	1092:1099	arg1	degradation					1056:1066	adjustable long-term degradation	1035:1066	adjustable long-term degradation	1035:1066	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	4	7	theme	SCPP	931:934	arg1	dosing					936:941	SCPP dosing	931:941	SCPP dosing	931:941	Scaffolds with increased SCPP concentrations showed higher particle density, lesser swelling ratio and greater biodegradability indicating that these critically important properties for bone healing are fine-tunable and highly dependent on SCPP dosing.					
36086517	4	8	theme	higher	743:748	arg1	density					759:765	higher particle density	743:765	higher particle density	743:765	Scaffolds with increased SCPP concentrations showed higher particle density, lesser swelling ratio and greater biodegradability indicating that these critically important properties for bone healing are fine-tunable and highly dependent on SCPP dosing.					
36086517	5	9	theme	alginate/collagen	998:1014	arg1	scaffolds					1016:1024	porous alginate/collagen scaffolds	991:1024	porous alginate/collagen scaffolds	991:1024	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	4	10	theme	SCPP	716:719	arg1	concentrations					721:734	increased SCPP concentrations	706:734	increased SCPP concentrations	706:734	Scaffolds with increased SCPP concentrations showed higher particle density, lesser swelling ratio and greater biodegradability indicating that these critically important properties for bone healing are fine-tunable and highly dependent on SCPP dosing.					
36086517	3	11	theme	varying	555:561	arg1	doses					563:567	varying doses	555:567	varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	555:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	5	12	theme	Clinical	944:951	arg1	dosing					968:973	Clinical Relevance- The dosing	944:973	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds	944:1024	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	4	13	theme	important	852:860	arg1	properties					862:871	these critically important properties	835:871	these critically important properties for bone healing	835:888	Scaffolds with increased SCPP concentrations showed higher particle density, lesser swelling ratio and greater biodegradability indicating that these critically important properties for bone healing are fine-tunable and highly dependent on SCPP dosing.					
36086517	4	14	theme	increased	706:714	arg1	concentrations					721:734	increased SCPP concentrations	706:734	increased SCPP concentrations	706:734	Scaffolds with increased SCPP concentrations showed higher particle density, lesser swelling ratio and greater biodegradability indicating that these critically important properties for bone healing are fine-tunable and highly dependent on SCPP dosing.					
36086517	3	15	from	L	598:598	arg1	doses					563:567	varying doses	555:567	varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	555:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	3	16	theme	fluid	677:681	arg1	uptake					683:688	fluid uptake	677:688	fluid uptake	677:688	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	2	17	theme	decreased	392:400	arg1	bioactivity					402:412	decreased bioactivity	392:412	decreased bioactivity	392:412	Biological scaffolds require bone extraction from the individual or an outside donor while synthetic grafts mostly suffer from poor degradation kinetics and decreased bioactivity.					
36086517	4	18	dep	fine-tunable	894:905	arg1	dependent					918:926	dependent	918:926	dependent	918:926	Scaffolds with increased SCPP concentrations showed higher particle density, lesser swelling ratio and greater biodegradability indicating that these critically important properties for bone healing are fine-tunable and highly dependent on SCPP dosing.					
36086517	5	19	theme	in	1115:1116	arg1	applications					1127:1138	potential in vivo CSD applications	1105:1138	potential in vivo CSD applications	1105:1138	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	4	20	theme	greater	794:800	arg1	biodegradability					802:817	greater biodegradability	794:817	greater biodegradability	794:817	Scaffolds with increased SCPP concentrations showed higher particle density, lesser swelling ratio and greater biodegradability indicating that these critically important properties for bone healing are fine-tunable and highly dependent on SCPP dosing.					
36086517	2	21	theme	bone	264:267	arg1	extraction					269:278	bone extraction	264:278	bone extraction from the individual	264:298	Biological scaffolds require bone extraction from the individual or an outside donor while synthetic grafts mostly suffer from poor degradation kinetics and decreased bioactivity.					
36086517	1	22	theme	bone	176:179	arg1	grafts					181:186	bone grafts	176:186	bone grafts	176:186	Treatment for critical size defects (CSDs) in bone often use bone grafts to act as a scaffold to help complete healing.					
36086517	5	23	theme	1	978:978	arg1	%					979:979	%	979:979	%	979:979	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	5	24	theme	%	979:979	arg1	SCPP					981:984	1% SCPP	978:984	1% SCPP	978:984	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	2	25	theme	outside	306:312	arg1	donor					314:318	an outside donor	303:318	an outside donor	303:318	Biological scaffolds require bone extraction from the individual or an outside donor while synthetic grafts mostly suffer from poor degradation kinetics and decreased bioactivity.					
36086517	5	26	theme	potential	1105:1113	arg1	applications					1127:1138	potential in vivo CSD applications	1105:1138	potential in vivo CSD applications	1105:1138	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	2	27	theme	degradation	367:377	arg1	kinetics					379:386	poor degradation kinetics	362:386	poor degradation kinetics	362:386	Biological scaffolds require bone extraction from the individual or an outside donor while synthetic grafts mostly suffer from poor degradation kinetics and decreased bioactivity.					
36086517	3	28	theme	L	583:583	arg1	L					598:598	2 m g/ m L to 20 m g/ m L	574:598	2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	574:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	1	29	from	defects	143:149	arg1	bone					161:164	bone	161:164	bone	161:164	Treatment for critical size defects (CSDs) in bone often use bone grafts to act as a scaffold to help complete healing.					
36086517	4	30	theme	swelling	775:782	arg1	ratio					784:788	lesser swelling ratio	768:788	lesser swelling ratio	768:788	Scaffolds with increased SCPP concentrations showed higher particle density, lesser swelling ratio and greater biodegradability indicating that these critically important properties for bone healing are fine-tunable and highly dependent on SCPP dosing.					
36086517	3	31	theme	printed	451:457	arg1	scaffold					459:466	a 3D printed scaffold	446:466	a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	446:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	1	32	theme	critical	129:136	arg1	CSDs					152:155	CSDs	152:155	CSDs	152:155	Treatment for critical size defects (CSDs) in bone often use bone grafts to act as a scaffold to help complete healing.					
36086517	1	32	theme	critical	129:136	arg1	defects					143:149	critical size defects	129:149	critical size defects (CSDs) in bone	129:164	Treatment for critical size defects (CSDs) in bone often use bone grafts to act as a scaffold to help complete healing.					
36086517	0	33	theme	Strontium-doped	42:56	arg1	Scaffolds					76:84	Strontium-doped Alginate/Collagen Scaffolds	42:84	Strontium-doped Alginate/Collagen Scaffolds	42:84	Controlled Biodegradation and Swelling of Strontium-doped Alginate/Collagen Scaffolds for Bone Tissue Engineering.					
36086517	3	34	theme	novel	483:487	arg1	bioink					499:504	a novel composite bioink	481:504	a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	481:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	5	35	theme	material	1072:1079	arg1	properties					1081:1090	material properties	1072:1090	material properties	1072:1090	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	3	36	theme	g/	578:579	arg1	L					598:598	2 m g/ m L to 20 m g/ m L	574:598	2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	574:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	5	37	dep	in	1115:1116	arg1	vivo					1118:1121	vivo	1118:1121	vivo	1118:1121	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	3	38	theme	g/	593:594	arg1	L					598:598	2 m g/ m L to 20 m g/ m L	574:598	2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	574:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	3	39	theme	m	581:581	arg1	L					598:598	2 m g/ m L to 20 m g/ m L	574:598	2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	574:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	3	40	theme	3D	448:449	arg1	scaffold					459:466	a 3D printed scaffold	446:466	a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	446:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	0	41	theme	Scaffolds	76:84	arg1	Swelling					30:37	Swelling	30:37	Swelling	30:37	Controlled Biodegradation and Swelling of Strontium-doped Alginate/Collagen Scaffolds for Bone Tissue Engineering.					
36086517	0	41	theme	Scaffolds	76:84	arg1	Biodegradation					11:24	Biodegradation	11:24	Biodegradation	11:24	Controlled Biodegradation and Swelling of Strontium-doped Alginate/Collagen Scaffolds for Bone Tissue Engineering.					
36086517	4	42	with	Scaffolds	691:699	arg1	concentrations					721:734	increased SCPP concentrations	706:734	increased SCPP concentrations	706:734	Scaffolds with increased SCPP concentrations showed higher particle density, lesser swelling ratio and greater biodegradability indicating that these critically important properties for bone healing are fine-tunable and highly dependent on SCPP dosing.					
36086517	4	43	theme	bone	877:880	arg1	healing					882:888	bone healing	877:888	bone healing	877:888	Scaffolds with increased SCPP concentrations showed higher particle density, lesser swelling ratio and greater biodegradability indicating that these critically important properties for bone healing are fine-tunable and highly dependent on SCPP dosing.					
36086517	3	44	theme	m	596:596	arg1	L					598:598	2 m g/ m L to 20 m g/ m L	574:598	2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	574:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	5	45	theme	Relevance-	953:962	arg1	dosing					968:973	Clinical Relevance- The dosing	944:973	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds	944:1024	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	1	46	theme	size	138:141	arg1	CSDs					152:155	CSDs	152:155	CSDs	152:155	Treatment for critical size defects (CSDs) in bone often use bone grafts to act as a scaffold to help complete healing.					
36086517	1	46	theme	size	138:141	arg1	defects					143:149	critical size defects	129:149	critical size defects (CSDs) in bone	129:164	Treatment for critical size defects (CSDs) in bone often use bone grafts to act as a scaffold to help complete healing.					
36086517	0	47	theme	Alginate/Collagen	58:74	arg1	Scaffolds					76:84	Strontium-doped Alginate/Collagen Scaffolds	42:84	Strontium-doped Alginate/Collagen Scaffolds	42:84	Controlled Biodegradation and Swelling of Strontium-doped Alginate/Collagen Scaffolds for Bone Tissue Engineering.					
36086517	3	48	theme	strontium-calcium	606:622	arg1	SCPP					639:642	SCPP	639:642	SCPP	639:642	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	3	48	theme	strontium-calcium	606:622	arg1	polyphosphate					624:636	1% strontium-calcium polyphosphate	603:636	1% strontium-calcium polyphosphate (SCPP)	603:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	3	49	theme	m	576:576	arg1	L					598:598	2 m g/ m L to 20 m g/ m L	574:598	2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	574:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	2	50	theme	synthetic	326:334	arg1	grafts					336:341	synthetic grafts	326:341	synthetic grafts	326:341	Biological scaffolds require bone extraction from the individual or an outside donor while synthetic grafts mostly suffer from poor degradation kinetics and decreased bioactivity.					
36086517	5	51	theme	porous	991:996	arg1	scaffolds					1016:1024	porous alginate/collagen scaffolds	991:1024	porous alginate/collagen scaffolds	991:1024	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	3	52	theme	polyphosphate	624:636	arg1	L					598:598	2 m g/ m L to 20 m g/ m L	574:598	2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	574:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	4	53	theme	particle	750:757	arg1	density					759:765	higher particle density	743:765	higher particle density	743:765	Scaffolds with increased SCPP concentrations showed higher particle density, lesser swelling ratio and greater biodegradability indicating that these critically important properties for bone healing are fine-tunable and highly dependent on SCPP dosing.					
36086517	4	54	theme	lesser	768:773	arg1	ratio					784:788	lesser swelling ratio	768:788	lesser swelling ratio	768:788	Scaffolds with increased SCPP concentrations showed higher particle density, lesser swelling ratio and greater biodegradability indicating that these critically important properties for bone healing are fine-tunable and highly dependent on SCPP dosing.					
36086517	5	55	theme	adjustable	1035:1044	arg1	degradation					1056:1066	adjustable long-term degradation	1035:1066	adjustable long-term degradation	1035:1066	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	3	56	theme	1	603:603	arg1	%					604:604	%	604:604	%	604:604	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	3	57	theme	m	591:591	arg1	L					598:598	2 m g/ m L to 20 m g/ m L	574:598	2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP)	574:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	0	58	theme	Bone	90:93	arg1	Engineering					102:112	Bone Tissue Engineering	90:112	Bone Tissue Engineering	90:112	Controlled Biodegradation and Swelling of Strontium-doped Alginate/Collagen Scaffolds for Bone Tissue Engineering.					
36086517	3	59	theme	%	604:604	arg1	SCPP					639:642	SCPP	639:642	SCPP	639:642	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	3	59	theme	%	604:604	arg1	polyphosphate					624:636	1% strontium-calcium polyphosphate	603:636	1% strontium-calcium polyphosphate (SCPP)	603:643	In this study, we investigated a 3D printed scaffold derived from a novel composite bioink composed of alginate and collagen augmented with varying doses from 2 m g/ m L to 20 m g/ m L of 1% strontium-calcium polyphosphate (SCPP) to control biodegradability and fluid uptake.					
36086517	2	60	theme	Biological	235:244	arg1	scaffolds					246:254	Biological scaffolds	235:254	Biological scaffolds	235:254	Biological scaffolds require bone extraction from the individual or an outside donor while synthetic grafts mostly suffer from poor degradation kinetics and decreased bioactivity.					
36086517	2	61	theme	poor	362:365	arg1	kinetics					379:386	poor degradation kinetics	362:386	poor degradation kinetics	362:386	Biological scaffolds require bone extraction from the individual or an outside donor while synthetic grafts mostly suffer from poor degradation kinetics and decreased bioactivity.					
36086517	1	62	theme	complete	217:224	arg1	healing					226:232	complete healing	217:232	complete healing	217:232	Treatment for critical size defects (CSDs) in bone often use bone grafts to act as a scaffold to help complete healing.					
36086517	5	63	theme	CSD	1123:1125	arg1	applications					1127:1138	potential in vivo CSD applications	1105:1138	potential in vivo CSD applications	1105:1138	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36086517	5	64	theme	SCPP	981:984	arg1	dosing					968:973	Clinical Relevance- The dosing	944:973	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds	944:1024	Clinical Relevance- The dosing of 1% SCPP into porous alginate/collagen scaffolds provides adjustable long-term degradation and material properties suitable for potential in vivo CSD applications.					
36255677	3	0	theme	constellation	310:322	arg1	occurrence					275:284	the occurrence	271:284	the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types	271:468	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	1	from	abnormalities	327:339	arg1	types					464:468	different CDG types	450:468	different CDG types	450:468	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	2	from	data	496:499	arg1	options					546:552	limited treatment options	528:552	limited treatment options	528:552	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	2	from	data	496:499	arg1	biomarkers					513:522	possible biomarkers	504:522	possible biomarkers	504:522	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	3	theme	limited	528:534	arg1	options					546:552	limited treatment options	528:552	limited treatment options	528:552	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	4	theme	possible	504:511	arg1	biomarkers					513:522	possible biomarkers	504:522	possible biomarkers	504:522	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	5	from	anomalies	371:379	arg1	neuroimaging					384:395	neuroimaging	384:395	neuroimaging	384:395	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	6	dep	discussed	474:482	arg1	followed					484:491	followed	484:491	followed by data on possible biomarkers and limited treatment options	484:552	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	7	theme	autistic	428:435	arg1	features					437:444	autistic features	428:444	autistic features	428:444	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	7	theme	autistic	428:435	arg1	epilepsy					355:362	epilepsy	355:362	epilepsy	355:362	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	8	from	types	464:468	arg1	constellation					310:322	various neurological constellation	289:322	various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types	289:468	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	9	theme	stroke-like	406:416	arg1	episodes					418:425	stroke-like episodes	406:425	stroke-like episodes	406:425	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	9	theme	stroke-like	406:416	arg1	epilepsy					355:362	epilepsy	355:362	epilepsy	355:362	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	0	10	theme	Neurological	0:11	arg1	Consequences					13:24	Neurological Consequences	0:24	Neurological Consequences of Congenital Disorders of Glycosylation	0:65	Neurological Consequences of Congenital Disorders of Glycosylation.					
36255677	1	11	theme	neurological	94:105	arg1	aspects					107:113	neurological aspects	94:113	neurological aspects of congenital disorders of glycosylation (CDG)	94:160	The chapter is devoted to neurological aspects of congenital disorders of glycosylation (CDG).					
36255677	0	12	theme	Congenital	29:38	arg1	Disorders					40:48	Congenital Disorders	29:48	Congenital Disorders of Glycosylation	29:65	Neurological Consequences of Congenital Disorders of Glycosylation.					
36255677	2	13	with	CDG	202:204	arg1	presentation					224:235	neurological presentation	211:235	neurological presentation of symptoms	211:247	At the beginning, the various types of CDG with neurological presentation of symptoms are summarized.					
36255677	3	14	theme	treatment	536:544	arg1	options					546:552	limited treatment options	528:552	limited treatment options	528:552	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	15	theme	brain	365:369	arg1	anomalies					371:379	brain anomalies	365:379	brain anomalies on neuroimaging	365:395	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	15	theme	brain	365:369	arg1	epilepsy					355:362	epilepsy	355:362	epilepsy	355:362	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	16	theme	different	450:458	arg1	types					464:468	different CDG types	450:468	different CDG types	450:468	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	0	17	theme	Disorders	40:48	arg1	Consequences					13:24	Neurological Consequences	0:24	Neurological Consequences of Congenital Disorders of Glycosylation	0:65	Neurological Consequences of Congenital Disorders of Glycosylation.					
36255677	2	18	theme	neurological	211:222	arg1	presentation					224:235	neurological presentation	211:235	neurological presentation of symptoms	211:247	At the beginning, the various types of CDG with neurological presentation of symptoms are summarized.					
36255677	3	19	theme	various	289:295	arg1	constellation					310:322	various neurological constellation	289:322	various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types	289:468	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	1	20	theme	congenital	118:127	arg1	disorders					129:137	congenital disorders	118:137	congenital disorders of glycosylation (CDG)	118:160	The chapter is devoted to neurological aspects of congenital disorders of glycosylation (CDG).					
36255677	0	21	theme	Glycosylation	53:65	arg1	Disorders					40:48	Congenital Disorders	29:48	Congenital Disorders of Glycosylation	29:65	Neurological Consequences of Congenital Disorders of Glycosylation.					
36255677	3	22	theme	neurological	297:308	arg1	constellation					310:322	various neurological constellation	289:322	various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types	289:468	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	3	23	from	constellation	310:322	arg1	types					464:468	different CDG types	450:468	different CDG types	450:468	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	2	24	theme	CDG	202:204	arg1	types					193:197	the various types	181:197	the various types of CDG with neurological presentation of symptoms	181:247	At the beginning, the various types of CDG with neurological presentation of symptoms are summarized.					
36255677	1	25	theme	disorders	129:137	arg1	aspects					107:113	neurological aspects	94:113	neurological aspects of congenital disorders of glycosylation (CDG)	94:160	The chapter is devoted to neurological aspects of congenital disorders of glycosylation (CDG).					
36255677	3	26	theme	CDG	460:462	arg1	types					464:468	different CDG types	450:468	different CDG types	450:468	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	2	27	theme	various	185:191	arg1	types					193:197	the various types	181:197	the various types of CDG with neurological presentation of symptoms	181:247	At the beginning, the various types of CDG with neurological presentation of symptoms are summarized.					
36255677	1	28	theme	glycosylation	142:154	arg1	disorders					129:137	congenital disorders	118:137	congenital disorders of glycosylation (CDG)	118:160	The chapter is devoted to neurological aspects of congenital disorders of glycosylation (CDG).					
36255677	3	29	theme	abnormalities	327:339	arg1	constellation					310:322	various neurological constellation	289:322	various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types	289:468	Then, the occurrence of various neurological constellation of abnormalities (for example: epilepsy, brain anomalies on neuroimaging, ataxia, stroke-like episodes, autistic features) in different CDG types are discussed followed by data on possible biomarkers and limited treatment options.					
36255677	2	30	theme	symptoms	240:247	arg1	presentation					224:235	neurological presentation	211:235	neurological presentation of symptoms	211:247	At the beginning, the various types of CDG with neurological presentation of symptoms are summarized.					
36422699	3	0	theme	higher	688:693	arg1	tolerance					752:760	a higher xylose fermentation capacity and fermentation inhibitors tolerance	686:760	a higher xylose fermentation capacity and fermentation inhibitors tolerance	686:760	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	9	1	theme	cellular	1508:1515	arg1	growth					1517:1522	cellular growth	1508:1522	cellular growth	1508:1522	Although UFFS-CE-3.1.2 fermented glucose efficiently, xylose and cellobiose were mainly used for cellular growth.					
36422699	4	2	contain	had	858:860	arg1	NaCl					853:856	NaCl	853:856	NaCl	853:856	Our results show that furfural and NaCl had no significant effect on sugar consumption by UFFS-CE-3.1.2.					
36422699	4	2	contain	had	858:860	arg1	furfural					840:847	furfural	840:847	furfural	840:847	Our results show that furfural and NaCl had no significant effect on sugar consumption by UFFS-CE-3.1.2.					
36422699	4	2	contain	had	858:860	arg2	effect					877:882	no significant effect	862:882	no significant effect	862:882	Our results show that furfural and NaCl had no significant effect on sugar consumption by UFFS-CE-3.1.2.					
36422699	6	3	theme	analyzed	1068:1075	arg1	responses					1077:1085	all the analyzed responses	1060:1085	all the analyzed responses	1060:1085	In contrast, the pH positively affected all the analyzed responses, indicating a cell's preference for alkaline environments.					
36422699	8	4	theme	inhibitors	1361:1370	arg1	8.0					1405:1407	8.0	1405:1407	8.0	1405:1407	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	8	4	theme	inhibitors	1361:1370	arg1	concentrations					1319:1332	the average concentrations	1307:1332	the average concentrations of sugars and fermentation inhibitors	1307:1370	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	8	4	theme	inhibitors	1361:1370	arg1	value					1398:1402	the highest tested pH value	1376:1402	the highest tested pH value (8.0)	1376:1408	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	0	5	theme	design	103:108	arg1	analysis					110:117	an experimental design analysis	87:117	Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.	0:118	Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.					
36422699	3	6	theme	optimized	492:500	arg1	condition					502:510	the optimized condition	488:510	the optimized condition	488:510	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	5	7	theme	acetic	937:942	arg1	acid					944:947	acetic acid	937:947	acetic acid	937:947	Surprisingly, acetic acid negatively affected glucose but not xylose and cellobiose consumption.					
36422699	0	8	theme	experimental	90:101	arg1	design					103:108	an experimental design	87:108	Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.	0:118	Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.					
36422699	3	9	theme	capacity	715:722	arg1	tolerance					752:760	a higher xylose fermentation capacity and fermentation inhibitors tolerance	686:760	a higher xylose fermentation capacity and fermentation inhibitors tolerance	686:760	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	7	10	theme	xylitol	1221:1227	arg1	yields					1202:1207	the yields	1198:1207	the yields of ethanol, xylitol, and cellular biomass	1198:1249	In the CCD, sugar concentration negatively affected the yields of ethanol, xylitol, and cellular biomass.					
36422699	8	11	theme	average	1311:1317	arg1	concentrations					1319:1332	the average concentrations	1307:1332	the average concentrations of sugars and fermentation inhibitors	1307:1370	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	7	12	theme	biomass	1243:1249	arg1	yields					1202:1207	the yields	1198:1207	the yields of ethanol, xylitol, and cellular biomass	1198:1249	In the CCD, sugar concentration negatively affected the yields of ethanol, xylitol, and cellular biomass.					
36422699	7	13	theme	sugar	1158:1162	arg1	concentration					1164:1176	sugar concentration	1158:1176	sugar concentration	1158:1176	In the CCD, sugar concentration negatively affected the yields of ethanol, xylitol, and cellular biomass.					
36422699	11	14	theme	it	1744:1745	arg1	it					1744:1745	it	1744:1745	it	1744:1745	Also, while UFFS-CE-3.1.2 only consumed 32% of the acetic acid of the medium, JDY-01 consumed > 60% of it, reducing its toxic effects.					
36422699	11	14	theme	it	1744:1745	arg1	%					1739:1739	> 60%	1735:1739	> 60% of it	1735:1745	Also, while UFFS-CE-3.1.2 only consumed 32% of the acetic acid of the medium, JDY-01 consumed > 60% of it, reducing its toxic effects.					
36422699	3	15	theme	composite	464:472	arg1	CCD					482:484	CCD	482:484	CCD	482:484	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	3	15	theme	composite	464:472	arg1	design					474:479	central composite design	456:479	central composite design (CCD)	456:485	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	8	16	theme	sugars	1337:1342	arg1	8.0					1405:1407	8.0	1405:1407	8.0	1405:1407	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	8	16	theme	sugars	1337:1342	arg1	concentrations					1319:1332	the average concentrations	1307:1332	the average concentrations of sugars and fermentation inhibitors	1307:1370	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	8	16	theme	sugars	1337:1342	arg1	value					1398:1402	the highest tested pH value	1376:1402	the highest tested pH value (8.0)	1376:1408	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	3	17	used	used	516:519	arg2	condition					502:510	the optimized condition	488:510	the optimized condition	488:510	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	3	18	theme	xylose	695:700	arg1	capacity					715:722	xylose fermentation capacity	695:722	xylose fermentation capacity	695:722	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	11	19	theme	medium	1711:1716	arg1	acid					1699:1702	the acetic acid	1688:1702	the acetic acid of the medium	1688:1716	Also, while UFFS-CE-3.1.2 only consumed 32% of the acetic acid of the medium, JDY-01 consumed > 60% of it, reducing its toxic effects.					
36422699	10	20	theme	engineered	1556:1565	arg1	JDY-01					1574:1579	the genetically engineered strain JDY-01	1540:1579	the genetically engineered strain JDY-01	1540:1579	Interestingly, the genetically engineered strain JDY-01 consumed ~ 30% more xylose and produced ~ 20% more ethanol.					
36422699	10	21	theme	more	1596:1599	arg1	xylose					1601:1606	~ 30% more xylose	1590:1606	~ 30% more xylose	1590:1606	Interestingly, the genetically engineered strain JDY-01 consumed ~ 30% more xylose and produced ~ 20% more ethanol.					
36422699	3	22	theme	fermentation	702:713	arg1	capacity					715:722	xylose fermentation capacity	695:722	xylose fermentation capacity	695:722	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	2	23	theme	carbon	333:338	arg1	sources					340:346	carbon sources	333:346	carbon sources	333:346	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	10	24	theme	more	1627:1630	arg1	ethanol					1632:1638	~ 20% more ethanol	1621:1638	~ 20% more ethanol	1621:1638	Interestingly, the genetically engineered strain JDY-01 consumed ~ 30% more xylose and produced ~ 20% more ethanol.					
36422699	11	25	theme	acid	1699:1702	arg1	%					1683:1683	32%	1681:1683	32% of the acetic acid of the medium	1681:1716	Also, while UFFS-CE-3.1.2 only consumed 32% of the acetic acid of the medium, JDY-01 consumed > 60% of it, reducing its toxic effects.					
36422699	11	25	theme	acid	1699:1702	arg1	acid					1699:1702	the acetic acid	1688:1702	the acetic acid of the medium	1688:1716	Also, while UFFS-CE-3.1.2 only consumed 32% of the acetic acid of the medium, JDY-01 consumed > 60% of it, reducing its toxic effects.					
36422699	9	26	theme	fermented	1434:1442	arg1	glucose					1444:1450	UFFS-CE-3.1.2 fermented glucose	1420:1450	UFFS-CE-3.1.2 fermented glucose	1420:1450	Although UFFS-CE-3.1.2 fermented glucose efficiently, xylose and cellobiose were mainly used for cellular growth.					
36422699	12	27	theme	2G	1915:1916	arg1	production					1926:1935	2G ethanol production	1915:1935	2G ethanol production	1915:1935	Thus, the overexpressed genes played an essential role in the inhibitors' tolerance, and the applied engineering strategy may help improve 2G ethanol production.					
36422699	3	28	theme	fermentation	526:537	arg1	analysis					547:554	a fermentation kinetic analysis	524:554	a fermentation kinetic analysis	524:554	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	3	29	theme	central	456:462	arg1	CCD					482:484	CCD	482:484	CCD	482:484	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	3	29	theme	central	456:462	arg1	design					474:479	central composite design	456:479	central composite design (CCD)	456:485	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	8	30	theme	fermentation	1348:1359	arg1	inhibitors					1361:1370	fermentation inhibitors	1348:1370	fermentation inhibitors	1348:1370	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	3	31	theme	fermentation	728:739	arg1	inhibitors					741:750	fermentation inhibitors	728:750	fermentation inhibitors	728:750	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	4	32	theme	sugar	887:891	arg1	consumption					893:903	sugar consumption	887:903	sugar consumption by UFFS-CE-3.1.2	887:920	Our results show that furfural and NaCl had no significant effect on sugar consumption by UFFS-CE-3.1.2.					
36422699	3	33	theme	kinetic	539:545	arg1	analysis					547:554	a fermentation kinetic analysis	524:554	a fermentation kinetic analysis	524:554	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	12	34	theme	ethanol	1918:1924	arg1	production					1926:1935	2G ethanol production	1915:1935	2G ethanol production	1915:1935	Thus, the overexpressed genes played an essential role in the inhibitors' tolerance, and the applied engineering strategy may help improve 2G ethanol production.					
36422699	1	35	theme	yeast	149:153	arg1	sp					171:172	The recently discovered wild yeast Wickerhamomyces sp	120:172	The recently discovered wild yeast Wickerhamomyces sp.	120:173	The recently discovered wild yeast Wickerhamomyces sp.					
36422699	0	36	theme	ethanol	39:45	arg1	production					47:56	ethanol production	39:56	ethanol production	39:56	Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.					
36422699	3	37	theme	inhibitors	741:750	arg1	tolerance					752:760	a higher xylose fermentation capacity and fermentation inhibitors tolerance	686:760	a higher xylose fermentation capacity and fermentation inhibitors tolerance	686:760	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	12	38	theme	essential	1816:1824	arg1	role					1826:1829	an essential role	1813:1829	an essential role	1813:1829	Thus, the overexpressed genes played an essential role in the inhibitors' tolerance, and the applied engineering strategy may help improve 2G ethanol production.					
36422699	8	39	theme	highest	1380:1386	arg1	8.0					1405:1407	8.0	1405:1407	8.0	1405:1407	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	8	39	theme	highest	1380:1386	arg1	value					1398:1402	the highest tested pH value	1376:1402	the highest tested pH value (8.0)	1376:1408	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	2	40	theme	fermentation	400:411	arg1	inhibitors					413:422	fermentation inhibitors	400:422	fermentation inhibitors	400:422	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	3	41	theme	Saccharomyces	611:623	arg1	strain					636:641	an industrial Saccharomyces cerevisiae strain	597:641	an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1	597:815	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	3	41	theme	Saccharomyces	611:623	arg1	JDY-01					644:649	JDY-01	644:649	JDY-01	644:649	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	2	42	theme	ethanol	259:265	arg1	yields					267:272	ethanol yields	259:272	ethanol yields	259:272	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	11	43	theme	toxic	1761:1765	arg1	effects					1767:1773	its toxic effects	1757:1773	its toxic effects	1757:1773	Also, while UFFS-CE-3.1.2 only consumed 32% of the acetic acid of the medium, JDY-01 consumed > 60% of it, reducing its toxic effects.					
36422699	4	44	theme	significant	865:875	arg1	effect					877:882	no significant effect	862:882	no significant effect	862:882	Our results show that furfural and NaCl had no significant effect on sugar consumption by UFFS-CE-3.1.2.					
36422699	7	45	theme	ethanol	1212:1218	arg1	yields					1202:1207	the yields	1198:1207	the yields of ethanol, xylitol, and cellular biomass	1198:1249	In the CCD, sugar concentration negatively affected the yields of ethanol, xylitol, and cellular biomass.					
36422699	8	46	theme	pH	1395:1396	arg1	8.0					1405:1407	8.0	1405:1407	8.0	1405:1407	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	8	46	theme	pH	1395:1396	arg1	value					1398:1402	the highest tested pH value	1376:1402	the highest tested pH value (8.0)	1376:1408	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	3	47	dep	genes	784:788	arg1	genes					784:788	the genes XYL1, XYL2, XKS1, and TAL1	780:815	the genes XYL1, XYL2, XKS1, and TAL1	780:815	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	3	47	dep	genes	784:788	arg1	XKS1					802:805	XKS1	802:805	XKS1	802:805	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	3	47	dep	genes	784:788	arg1	TAL1					812:815	TAL1	812:815	TAL1	812:815	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	3	47	dep	genes	784:788	arg1	XYL2					796:799	XYL2	796:799	XYL2	796:799	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	3	47	dep	genes	784:788	arg1	XYL1					790:793	XYL1	790:793	XYL1	790:793	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	8	48	theme	fermentation	1263:1274	arg1	kinetics					1276:1283	fermentation kinetics	1263:1283	fermentation kinetics	1263:1283	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	12	49	theme	engineering	1877:1887	arg1	strategy					1889:1896	the applied engineering strategy	1865:1896	the applied engineering strategy	1865:1896	Thus, the overexpressed genes played an essential role in the inhibitors' tolerance, and the applied engineering strategy may help improve 2G ethanol production.					
36422699	11	50	theme	acetic	1692:1697	arg1	acid					1699:1702	the acetic acid	1688:1702	the acetic acid of the medium	1688:1716	Also, while UFFS-CE-3.1.2 only consumed 32% of the acetic acid of the medium, JDY-01 consumed > 60% of it, reducing its toxic effects.					
36422699	8	51	theme	tested	1388:1393	arg1	8.0					1405:1407	8.0	1405:1407	8.0	1405:1407	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	8	51	theme	tested	1388:1393	arg1	value					1398:1402	the highest tested pH value	1376:1402	the highest tested pH value (8.0)	1376:1408	Therefore, fermentation kinetics were carried out with the average concentrations of sugars and fermentation inhibitors and the highest tested pH value (8.0).					
36422699	2	52	theme	acetic	352:357	arg1	acid					359:362	acetic acid	352:362	acetic acid	352:362	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	2	53	with	media	287:291	arg1	cellobiose					319:328	cellobiose	319:328	cellobiose	319:328	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	2	53	with	media	287:291	arg1	NaCl					392:395	NaCl	392:395	NaCl	392:395	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	2	53	with	media	287:291	arg1	furfural					365:372	furfural	365:372	furfural	365:372	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	2	53	with	media	287:291	arg1	glucose					298:304	glucose	298:304	glucose	298:304	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	2	53	with	media	287:291	arg1	xylose					307:312	xylose	307:312	xylose	307:312	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	2	53	with	media	287:291	arg1	acid					382:385	formic acid	375:385	formic acid	375:385	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	2	53	with	media	287:291	arg1	acid					359:362	acetic acid	352:362	acetic acid	352:362	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	12	54	theme	overexpressed	1786:1798	arg1	genes					1800:1804	the overexpressed genes	1782:1804	the overexpressed genes	1782:1804	Thus, the overexpressed genes played an essential role in the inhibitors' tolerance, and the applied engineering strategy may help improve 2G ethanol production.					
36422699	2	55	theme	experimental	228:239	arg1	design					241:246	a high-throughput experimental design	210:246	a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors	210:422	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	1	56	theme	discovered	133:142	arg1	sp					171:172	The recently discovered wild yeast Wickerhamomyces sp	120:172	The recently discovered wild yeast Wickerhamomyces sp.	120:173	The recently discovered wild yeast Wickerhamomyces sp.					
36422699	6	57	theme	alkaline	1123:1130	arg1	environments					1132:1143	alkaline environments	1123:1143	alkaline environments	1123:1143	In contrast, the pH positively affected all the analyzed responses, indicating a cell's preference for alkaline environments.					
36422699	3	58	dep	Saccharomyces	611:623	arg1	cerevisiae					625:634	cerevisiae	625:634	cerevisiae	625:634	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	2	59	theme	high-throughput	212:226	arg1	design					241:246	a high-throughput experimental design	210:246	a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors	210:422	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	3	60	theme	industrial	600:609	arg1	strain					636:641	an industrial Saccharomyces cerevisiae strain	597:641	an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1	597:815	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	3	60	theme	industrial	600:609	arg1	JDY-01					644:649	JDY-01	644:649	JDY-01	644:649	After Plackett-Burman (PB) and central composite design (CCD), the optimized condition was used in a fermentation kinetic analysis to compare this yeast's performance with an industrial Saccharomyces cerevisiae strain (JDY-01) genetically engineered to achieve a higher xylose fermentation capacity and fermentation inhibitors tolerance by overexpressing the genes XYL1, XYL2, XKS1, and TAL1.					
36422699	1	61	theme	wild	144:147	arg1	sp					171:172	The recently discovered wild yeast Wickerhamomyces sp	120:172	The recently discovered wild yeast Wickerhamomyces sp.	120:173	The recently discovered wild yeast Wickerhamomyces sp.					
36422699	7	62	theme	cellular	1234:1241	arg1	biomass					1243:1249	cellular biomass	1234:1249	cellular biomass	1234:1249	In the CCD, sugar concentration negatively affected the yields of ethanol, xylitol, and cellular biomass.					
36422699	2	63	theme	synthetic	277:285	arg1	media					287:291	synthetic media	277:291	synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors	277:422	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	5	64	dep	xylose	985:990	arg1	consumption					1007:1017	consumption	1007:1017	consumption	1007:1017	Surprisingly, acetic acid negatively affected glucose but not xylose and cellobiose consumption.					
36422699	2	65	theme	formic	375:380	arg1	acid					382:385	formic acid	375:385	formic acid	375:385	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	9	66	used	used	1499:1502	arg2	xylose					1465:1470	xylose	1465:1470	xylose	1465:1470	Although UFFS-CE-3.1.2 fermented glucose efficiently, xylose and cellobiose were mainly used for cellular growth.					
36422699	9	66	used	used	1499:1502	arg2	cellobiose					1476:1485	cellobiose	1476:1485	cellobiose	1476:1485	Although UFFS-CE-3.1.2 fermented glucose efficiently, xylose and cellobiose were mainly used for cellular growth.					
36422699	2	67	dep	design	241:246	arg1	improve					251:257	improve	251:257	to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors	248:422	UFFS-CE-3.1.2 was analyzed through a high-throughput experimental design to improve ethanol yields in synthetic media with glucose, xylose, and cellobiose as carbon sources and acetic acid, furfural, formic acid, and NaCl as fermentation inhibitors.					
36422699	0	68	theme	inhibitory	64:73	arg1	conditions					75:84	inhibitory conditions	64:84	inhibitory conditions	64:84	Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.					
36422699	0	69	dep	Prospecting	0:10	arg1	yeasts					28:33	yeasts	28:33	yeasts	28:33	Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.					
36422699	0	69	dep	Prospecting	0:10	arg1	analysis					110:117	an experimental design analysis	87:117	Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.	0:118	Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.					
36422699	12	70	theme	applied	1869:1875	arg1	strategy					1889:1896	the applied engineering strategy	1865:1896	the applied engineering strategy	1865:1896	Thus, the overexpressed genes played an essential role in the inhibitors' tolerance, and the applied engineering strategy may help improve 2G ethanol production.					
36422699	1	71	theme	Wickerhamomyces	155:169	arg1	sp					171:172	The recently discovered wild yeast Wickerhamomyces sp	120:172	The recently discovered wild yeast Wickerhamomyces sp.	120:173	The recently discovered wild yeast Wickerhamomyces sp.					
36422699	10	72	theme	strain	1567:1572	arg1	JDY-01					1574:1579	the genetically engineered strain JDY-01	1540:1579	the genetically engineered strain JDY-01	1540:1579	Interestingly, the genetically engineered strain JDY-01 consumed ~ 30% more xylose and produced ~ 20% more ethanol.					
36841370	9	0	theme	complete	1510:1517	arg1	recovery					1519:1526	a complete recovery	1508:1526	a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers	1508:1624	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	10	1	theme	efficient	1772:1780	arg1	management					1794:1803	efficient transdermal management	1772:1803	efficient transdermal management of joint arthritis	1772:1822	Therefore, OFP represents a competent nanocarrier for efficient transdermal management of joint arthritis.					
36841370	3	2	theme	factors	520:526	arg1	optimization					502:513	the optimization	498:513	the optimization of 4 factors	498:526	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	6	3	theme	µg/cm2	1093:1098	arg1	/24h					1100:1103	16.21 µg/cm2 /24h	1087:1103	16.21 µg/cm2 /24h for Y1, Y2, Y3, and Y4	1087:1126	The optimal formula was formulated using 2.99% phospholipon90G, 0.04% sodium deoxycholate, and 0.29% stearylamine, and showed 84.72%, 268.13 nm, 5.89 µg/cm2/h, and 16.21 µg/cm2 /24h for Y1, Y2, Y3, and Y4, respectively.					
36841370	9	4	theme	normal	1535:1540	arg1	structure					1555:1563	the normal histological structure	1531:1563	the normal histological structure of a rat joint and normal levels of the inflammatory markers	1531:1624	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	10	5	theme	joint	1808:1812	arg1	arthritis					1814:1822	joint arthritis	1808:1822	joint arthritis	1808:1822	Therefore, OFP represents a competent nanocarrier for efficient transdermal management of joint arthritis.					
36841370	3	6	theme	entrapment	546:555	arg1	efficiency					557:566	entrapment efficiency	546:566	entrapment efficiency (Y1)	546:571	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	3	6	theme	entrapment	546:555	arg1	Y1					569:570	Y1	569:570	Y1	569:570	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	9	7	theme	structure	1555:1563	arg1	recovery					1519:1526	a complete recovery	1508:1526	a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers	1508:1624	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	4	8	theme	cultivate®	833:842	arg1	cream					844:848	cultivate® cream	833:848	cultivate® cream	833:848	The OFP was characterized against the drug suspension, loaded into a Carbopol gel, and a histopathological assessment was conducted on a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel.					
36841370	2	9	theme	formula	312:318	arg1	optimization					265:276	optimization	265:276	optimization	265:276	This study focused on the development and optimization of a cationic nanosized bilosomal formula for the efficient transdermal treatment of arthritis.					
36841370	2	9	theme	formula	312:318	arg1	development					249:259	development	249:259	development	249:259	This study focused on the development and optimization of a cationic nanosized bilosomal formula for the efficient transdermal treatment of arthritis.					
36841370	7	10	theme	amorphous	1262:1270	arg1	drug					1272:1275	the amorphous drug	1258:1275	the amorphous drug	1258:1275	The thermal analysis of OFP demonstrated a single broad endothermic peak for bilosomes with no detectable peak for the amorphous drug.					
36841370	2	11	theme	nanosized	292:300	arg1	formula					312:318	a cationic nanosized bilosomal formula	281:318	a cationic nanosized bilosomal formula for the efficient transdermal treatment of arthritis	281:371	This study focused on the development and optimization of a cationic nanosized bilosomal formula for the efficient transdermal treatment of arthritis.					
36841370	9	12	theme	joint	1574:1578	arg1	structure					1555:1563	the normal histological structure	1531:1563	the normal histological structure of a rat joint and normal levels of the inflammatory markers	1531:1624	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	7	13	theme	endothermic	1199:1209	arg1	peak					1211:1214	a single broad endothermic peak	1184:1214	a single broad endothermic peak for bilosomes with no detectable peak for the amorphous drug	1184:1275	The thermal analysis of OFP demonstrated a single broad endothermic peak for bilosomes with no detectable peak for the amorphous drug.					
36841370	1	14	theme	life	217:220	arg1	quality					206:212	quality	206:212	quality	206:212	Arthritis is a debilitating disease that affects the patient's mobility and quality of life.					
36841370	1	14	theme	life	217:220	arg1	mobility					193:200	patient's mobility	183:200	patient's mobility	183:200	Arthritis is a debilitating disease that affects the patient's mobility and quality of life.					
36841370	9	15	theme	normal	1584:1589	arg1	levels					1591:1596	normal levels	1584:1596	normal levels of the inflammatory markers	1584:1624	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	7	16	theme	detectable	1238:1247	arg1	peak					1249:1252	no detectable peak	1235:1252	no detectable peak for the amorphous drug	1235:1275	The thermal analysis of OFP demonstrated a single broad endothermic peak for bilosomes with no detectable peak for the amorphous drug.					
36841370	4	17	theme	traditional	854:864	arg1	gel					866:868	traditional gel	854:868	traditional gel	854:868	The OFP was characterized against the drug suspension, loaded into a Carbopol gel, and a histopathological assessment was conducted on a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel.					
36841370	8	18	theme	OFP	1340:1342	arg1	structures					1312:1321	the spherical structures	1298:1321	the spherical structures of the nanosized OFP	1298:1342	TEM images revealed the spherical structures of the nanosized OFP, while CLSM demonstrated enhanced permeation efficiency over the drug suspension.					
36841370	3	19	theme	efficiency	557:566	arg1	design					482:487	the Draper-Lin small composite design	451:487	the Draper-Lin small composite design based on the optimization of 4 factors	451:526	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	3	19	theme	efficiency	557:566	arg1	evaluation					532:541	evaluation	532:541	evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4)	532:634	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	9	20	theme	inflammatory	1605:1616	arg1	markers					1618:1624	the inflammatory markers	1601:1624	the inflammatory markers	1601:1624	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	7	21	with	bilosomes	1220:1228	arg1	peak					1249:1252	no detectable peak	1235:1252	no detectable peak for the amorphous drug	1235:1275	The thermal analysis of OFP demonstrated a single broad endothermic peak for bilosomes with no detectable peak for the amorphous drug.					
36841370	3	22	theme	skin	593:596	arg1	Y3					604:605	Y3	604:605	Y3	604:605	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	3	22	theme	skin	593:596	arg1	flux					598:601	skin flux	593:601	skin flux (Y3)	593:606	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	6	23	theme	2.99	964:967	arg1	%					968:968	%	968:968	%	968:968	The optimal formula was formulated using 2.99% phospholipon90G, 0.04% sodium deoxycholate, and 0.29% stearylamine, and showed 84.72%, 268.13 nm, 5.89 µg/cm2/h, and 16.21 µg/cm2 /24h for Y1, Y2, Y3, and Y4, respectively.					
36841370	0	24	theme	rat	84:86	arg1	arthritis					88:96	carrageenan-induced rat arthritis	64:96	carrageenan-induced rat arthritis	64:96	Therapeutic potential of cationic bilosomes in the treatment of carrageenan-induced rat arthritis via fluticasone propionate gel.					
36841370	8	25	theme	permeation	1378:1387	arg1	efficiency					1389:1398	enhanced permeation efficiency	1369:1398	enhanced permeation efficiency	1369:1398	TEM images revealed the spherical structures of the nanosized OFP, while CLSM demonstrated enhanced permeation efficiency over the drug suspension.					
36841370	0	26	theme	propionate	114:123	arg1	gel					125:127	fluticasone propionate gel	102:127	fluticasone propionate gel	102:127	Therapeutic potential of cationic bilosomes in the treatment of carrageenan-induced rat arthritis via fluticasone propionate gel.					
36841370	6	27	theme	0.29	1018:1021	arg1	%					1022:1022	%	1022:1022	%	1022:1022	The optimal formula was formulated using 2.99% phospholipon90G, 0.04% sodium deoxycholate, and 0.29% stearylamine, and showed 84.72%, 268.13 nm, 5.89 µg/cm2/h, and 16.21 µg/cm2 /24h for Y1, Y2, Y3, and Y4, respectively.					
36841370	3	28	theme	Propionate-loaded	397:413	arg1	OFP					426:428	OFP	426:428	OFP	426:428	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	3	28	theme	Propionate-loaded	397:413	arg1	bilosomes					415:423	An optimum Fluticasone Propionate-loaded bilosomes	374:423	An optimum Fluticasone Propionate-loaded bilosomes (OFP)	374:429	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	8	29	theme	drug	1409:1412	arg1	suspension					1414:1423	the drug suspension	1405:1423	the drug suspension	1405:1423	TEM images revealed the spherical structures of the nanosized OFP, while CLSM demonstrated enhanced permeation efficiency over the drug suspension.					
36841370	0	30	theme	Therapeutic	0:10	arg1	potential					12:20	Therapeutic potential	0:20	Therapeutic potential of cationic	0:32	Therapeutic potential of cationic bilosomes in the treatment of carrageenan-induced rat arthritis via fluticasone propionate gel.					
36841370	4	31	theme	carrageenan-induced	774:792	arg1	arthritis					804:812	a carrageenan-induced rat joint arthritis	772:812	a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel	772:868	The OFP was characterized against the drug suspension, loaded into a Carbopol gel, and a histopathological assessment was conducted on a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel.					
36841370	7	32	theme	broad	1193:1197	arg1	peak					1211:1214	a single broad endothermic peak	1184:1214	a single broad endothermic peak for bilosomes with no detectable peak for the amorphous drug	1184:1275	The thermal analysis of OFP demonstrated a single broad endothermic peak for bilosomes with no detectable peak for the amorphous drug.					
36841370	3	33	theme	optimum	377:383	arg1	OFP					426:428	OFP	426:428	OFP	426:428	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	3	33	theme	optimum	377:383	arg1	bilosomes					415:423	An optimum Fluticasone Propionate-loaded bilosomes	374:423	An optimum Fluticasone Propionate-loaded bilosomes (OFP)	374:429	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	9	34	theme	promising	1463:1471	arg1	efficacy					1473:1480	the promising efficacy	1459:1480	the promising efficacy of the optimum OFP	1459:1499	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	6	35	theme	sodium	993:998	arg1	deoxycholate					1000:1011	0.04% sodium deoxycholate	987:1011	0.04% sodium deoxycholate	987:1011	The optimal formula was formulated using 2.99% phospholipon90G, 0.04% sodium deoxycholate, and 0.29% stearylamine, and showed 84.72%, 268.13 nm, 5.89 µg/cm2/h, and 16.21 µg/cm2 /24h for Y1, Y2, Y3, and Y4, respectively.					
36841370	3	36	theme	small	466:470	arg1	design					482:487	the Draper-Lin small composite design	451:487	the Draper-Lin small composite design based on the optimization of 4 factors	451:526	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	7	37	theme	OFP	1167:1169	arg1	analysis					1155:1162	The thermal analysis	1143:1162	The thermal analysis of OFP	1143:1169	The thermal analysis of OFP demonstrated a single broad endothermic peak for bilosomes with no detectable peak for the amorphous drug.					
36841370	9	38	theme	bilosomal	1703:1711	arg1	gel					1713:1715	the optimum bilosomal gel	1691:1715	the optimum bilosomal gel	1691:1715	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	8	39	theme	TEM	1278:1280	arg1	images					1282:1287	TEM images	1278:1287	TEM images	1278:1287	TEM images revealed the spherical structures of the nanosized OFP, while CLSM demonstrated enhanced permeation efficiency over the drug suspension.					
36841370	9	40	theme	optimum	1489:1495	arg1	OFP					1497:1499	the optimum OFP	1485:1499	the optimum OFP	1485:1499	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	10	41	theme	competent	1746:1754	arg1	nanocarrier					1756:1766	a competent nanocarrier	1744:1766	a competent nanocarrier for efficient transdermal management of joint arthritis	1744:1822	Therefore, OFP represents a competent nanocarrier for efficient transdermal management of joint arthritis.					
36841370	2	42	theme	efficient	328:336	arg1	treatment					350:358	the efficient transdermal treatment	324:358	the efficient transdermal treatment of arthritis	324:371	This study focused on the development and optimization of a cationic nanosized bilosomal formula for the efficient transdermal treatment of arthritis.					
36841370	3	43	theme	accumulation	618:629	arg1	design					482:487	the Draper-Lin small composite design	451:487	the Draper-Lin small composite design based on the optimization of 4 factors	451:526	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	3	43	theme	accumulation	618:629	arg1	evaluation					532:541	evaluation	532:541	evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4)	532:634	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	8	44	theme	spherical	1302:1310	arg1	structures					1312:1321	the spherical structures	1298:1321	the spherical structures of the nanosized OFP	1298:1342	TEM images revealed the spherical structures of the nanosized OFP, while CLSM demonstrated enhanced permeation efficiency over the drug suspension.					
36841370	10	45	theme	transdermal	1782:1792	arg1	management					1794:1803	efficient transdermal management	1772:1803	efficient transdermal management of joint arthritis	1772:1822	Therefore, OFP represents a competent nanocarrier for efficient transdermal management of joint arthritis.					
36841370	2	46	theme	arthritis	363:371	arg1	treatment					350:358	the efficient transdermal treatment	324:358	the efficient transdermal treatment of arthritis	324:371	This study focused on the development and optimization of a cationic nanosized bilosomal formula for the efficient transdermal treatment of arthritis.					
36841370	4	47	theme	joint	798:802	arg1	arthritis					804:812	a carrageenan-induced rat joint arthritis	772:812	a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel	772:868	The OFP was characterized against the drug suspension, loaded into a Carbopol gel, and a histopathological assessment was conducted on a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel.					
36841370	2	48	theme	cationic	283:290	arg1	formula					312:318	a cationic nanosized bilosomal formula	281:318	a cationic nanosized bilosomal formula for the efficient transdermal treatment of arthritis	281:371	This study focused on the development and optimization of a cationic nanosized bilosomal formula for the efficient transdermal treatment of arthritis.					
36841370	6	49	theme	16.21	1087:1091	arg1	/24h					1100:1103	16.21 µg/cm2 /24h	1087:1103	16.21 µg/cm2 /24h for Y1, Y2, Y3, and Y4	1087:1126	The optimal formula was formulated using 2.99% phospholipon90G, 0.04% sodium deoxycholate, and 0.29% stearylamine, and showed 84.72%, 268.13 nm, 5.89 µg/cm2/h, and 16.21 µg/cm2 /24h for Y1, Y2, Y3, and Y4, respectively.					
36841370	10	50	theme	arthritis	1814:1822	arg1	management					1794:1803	efficient transdermal management	1772:1803	efficient transdermal management of joint arthritis	1772:1822	Therefore, OFP represents a competent nanocarrier for efficient transdermal management of joint arthritis.					
36841370	9	51	theme	histological	1542:1553	arg1	structure					1555:1563	the normal histological structure	1531:1563	the normal histological structure of a rat joint and normal levels of the inflammatory markers	1531:1624	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	6	52	theme	optimal	927:933	arg1	formula					935:941	The optimal formula	923:941	The optimal formula	923:941	The optimal formula was formulated using 2.99% phospholipon90G, 0.04% sodium deoxycholate, and 0.29% stearylamine, and showed 84.72%, 268.13 nm, 5.89 µg/cm2/h, and 16.21 µg/cm2 /24h for Y1, Y2, Y3, and Y4, respectively.					
36841370	4	53	theme	rat	794:796	arg1	arthritis					804:812	a carrageenan-induced rat joint arthritis	772:812	a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel	772:868	The OFP was characterized against the drug suspension, loaded into a Carbopol gel, and a histopathological assessment was conducted on a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel.					
36841370	2	54	dep	development	249:259	arg1	the					245:247	the	245:247	the	245:247	This study focused on the development and optimization of a cationic nanosized bilosomal formula for the efficient transdermal treatment of arthritis.					
36841370	2	55	theme	bilosomal	302:310	arg1	formula					312:318	a cationic nanosized bilosomal formula	281:318	a cationic nanosized bilosomal formula for the efficient transdermal treatment of arthritis	281:371	This study focused on the development and optimization of a cationic nanosized bilosomal formula for the efficient transdermal treatment of arthritis.					
36841370	3	56	theme	vesicle	574:580	arg1	Y2					588:589	Y2	588:589	Y2	588:589	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	3	56	theme	vesicle	574:580	arg1	size					582:585	vesicle size	574:585	vesicle size (Y2)	574:590	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	9	57	theme	rat	1570:1572	arg1	joint					1574:1578	a rat joint	1568:1578	a rat joint	1568:1578	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	8	58	theme	nanosized	1330:1338	arg1	OFP					1340:1342	the nanosized OFP	1326:1342	the nanosized OFP	1326:1342	TEM images revealed the spherical structures of the nanosized OFP, while CLSM demonstrated enhanced permeation efficiency over the drug suspension.					
36841370	6	59	theme	0.04	987:990	arg1	%					991:991	%	991:991	%	991:991	The optimal formula was formulated using 2.99% phospholipon90G, 0.04% sodium deoxycholate, and 0.29% stearylamine, and showed 84.72%, 268.13 nm, 5.89 µg/cm2/h, and 16.21 µg/cm2 /24h for Y1, Y2, Y3, and Y4, respectively.					
36841370	3	60	theme	size	582:585	arg1	design					482:487	the Draper-Lin small composite design	451:487	the Draper-Lin small composite design based on the optimization of 4 factors	451:526	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	3	60	theme	size	582:585	arg1	evaluation					532:541	evaluation	532:541	evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4)	532:634	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	9	61	theme	levels	1591:1596	arg1	structure					1555:1563	the normal histological structure	1531:1563	the normal histological structure of a rat joint and normal levels of the inflammatory markers	1531:1624	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	3	62	theme	flux	598:601	arg1	design					482:487	the Draper-Lin small composite design	451:487	the Draper-Lin small composite design based on the optimization of 4 factors	451:526	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	3	62	theme	flux	598:601	arg1	evaluation					532:541	evaluation	532:541	evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4)	532:634	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	5	63	theme	Interluekin-1β	871:884	arg1	levels					896:901	Interluekin-1β and TNF-α levels	871:901	Interluekin-1β and TNF-α levels	871:901	Interluekin-1β and TNF-α levels were also measured.					
36841370	4	64	with	comparison	817:826	arg1	cream					844:848	cultivate® cream	833:848	cultivate® cream	833:848	The OFP was characterized against the drug suspension, loaded into a Carbopol gel, and a histopathological assessment was conducted on a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel.					
36841370	4	64	with	comparison	817:826	arg1	gel					866:868	traditional gel	854:868	traditional gel	854:868	The OFP was characterized against the drug suspension, loaded into a Carbopol gel, and a histopathological assessment was conducted on a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel.					
36841370	0	65	theme	carrageenan-induced	64:82	arg1	arthritis					88:96	carrageenan-induced rat arthritis	64:96	carrageenan-induced rat arthritis	64:96	Therapeutic potential of cationic bilosomes in the treatment of carrageenan-induced rat arthritis via fluticasone propionate gel.					
36841370	1	66	theme	debilitating	145:156	arg1	disease					158:164	a debilitating disease	143:164	a debilitating disease that affects the patient's mobility and quality of life	143:220	Arthritis is a debilitating disease that affects the patient's mobility and quality of life.					
36841370	1	66	theme	debilitating	145:156	arg1	Arthritis					130:138	Arthritis	130:138	Arthritis	130:138	Arthritis is a debilitating disease that affects the patient's mobility and quality of life.					
36841370	6	67	theme	%	968:968	arg1	phospholipon90G					970:984	2.99% phospholipon90G	964:984	2.99% phospholipon90G	964:984	The optimal formula was formulated using 2.99% phospholipon90G, 0.04% sodium deoxycholate, and 0.29% stearylamine, and showed 84.72%, 268.13 nm, 5.89 µg/cm2/h, and 16.21 µg/cm2 /24h for Y1, Y2, Y3, and Y4, respectively.					
36841370	9	68	theme	markers	1618:1624	arg1	joint					1574:1578	a rat joint	1568:1578	a rat joint	1568:1578	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	9	68	theme	markers	1618:1624	arg1	levels					1591:1596	normal levels	1584:1596	normal levels of the inflammatory markers	1584:1624	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	8	69	theme	enhanced	1369:1376	arg1	efficiency					1389:1398	enhanced permeation efficiency	1369:1398	enhanced permeation efficiency	1369:1398	TEM images revealed the spherical structures of the nanosized OFP, while CLSM demonstrated enhanced permeation efficiency over the drug suspension.					
36841370	0	70	theme	arthritis	88:96	arg1	treatment					51:59	the treatment	47:59	the treatment of carrageenan-induced rat arthritis	47:96	Therapeutic potential of cationic bilosomes in the treatment of carrageenan-induced rat arthritis via fluticasone propionate gel.					
36841370	0	71	theme	fluticasone	102:112	arg1	propionate					114:123	fluticasone propionate	102:123	fluticasone propionate gel	102:127	Therapeutic potential of cationic bilosomes in the treatment of carrageenan-induced rat arthritis via fluticasone propionate gel.					
36841370	4	72	from	arthritis	804:812	arg1	comparison					817:826	comparison	817:826	comparison with cultivate® cream and traditional gel	817:868	The OFP was characterized against the drug suspension, loaded into a Carbopol gel, and a histopathological assessment was conducted on a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel.					
36841370	9	73	located	observed	1631:1638	arg1	days					1650:1653	20 days	1647:1653	20 days	1647:1653	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	9	73	located	observed	1631:1638	arg2	recovery					1519:1526	a complete recovery	1508:1526	a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers	1508:1624	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	6	74	theme	%	1022:1022	arg1	stearylamine					1024:1035	0.29% stearylamine	1018:1035	0.29% stearylamine	1018:1035	The optimal formula was formulated using 2.99% phospholipon90G, 0.04% sodium deoxycholate, and 0.29% stearylamine, and showed 84.72%, 268.13 nm, 5.89 µg/cm2/h, and 16.21 µg/cm2 /24h for Y1, Y2, Y3, and Y4, respectively.					
36841370	4	75	theme	Carbopol	706:713	arg1	gel					715:717	a Carbopol gel	704:717	a Carbopol gel	704:717	The OFP was characterized against the drug suspension, loaded into a Carbopol gel, and a histopathological assessment was conducted on a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel.					
36841370	3	76	theme	Draper-Lin	455:464	arg1	design					482:487	the Draper-Lin small composite design	451:487	the Draper-Lin small composite design based on the optimization of 4 factors	451:526	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	5	77	theme	TNF-α	890:894	arg1	levels					896:901	Interluekin-1β and TNF-α levels	871:901	Interluekin-1β and TNF-α levels	871:901	Interluekin-1β and TNF-α levels were also measured.					
36841370	7	78	theme	single	1186:1191	arg1	peak					1211:1214	a single broad endothermic peak	1184:1214	a single broad endothermic peak for bilosomes with no detectable peak for the amorphous drug	1184:1275	The thermal analysis of OFP demonstrated a single broad endothermic peak for bilosomes with no detectable peak for the amorphous drug.					
36841370	9	79	theme	daily	1670:1674	arg1	application					1676:1686	once daily application	1665:1686	once daily application of the optimum bilosomal gel	1665:1715	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	4	80	theme	drug	675:678	arg1	suspension					680:689	the drug suspension	671:689	the drug suspension	671:689	The OFP was characterized against the drug suspension, loaded into a Carbopol gel, and a histopathological assessment was conducted on a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel.					
36841370	0	81	theme	cationic	25:32	arg1	potential					12:20	Therapeutic potential	0:20	Therapeutic potential of cationic	0:32	Therapeutic potential of cationic bilosomes in the treatment of carrageenan-induced rat arthritis via fluticasone propionate gel.					
36841370	4	82	theme	histopathological	726:742	arg1	assessment					744:753	a histopathological assessment	724:753	a histopathological assessment	724:753	The OFP was characterized against the drug suspension, loaded into a Carbopol gel, and a histopathological assessment was conducted on a carrageenan-induced rat joint arthritis in comparison with cultivate® cream and traditional gel.					
36841370	3	83	theme	Fluticasone	385:395	arg1	OFP					426:428	OFP	426:428	OFP	426:428	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	3	83	theme	Fluticasone	385:395	arg1	bilosomes					415:423	An optimum Fluticasone Propionate-loaded bilosomes	374:423	An optimum Fluticasone Propionate-loaded bilosomes (OFP)	374:429	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	9	84	theme	in-vivo	1430:1436	arg1	study					1438:1442	The in-vivo study	1426:1442	The in-vivo study	1426:1442	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	6	85	theme	%	991:991	arg1	deoxycholate					1000:1011	0.04% sodium deoxycholate	987:1011	0.04% sodium deoxycholate	987:1011	The optimal formula was formulated using 2.99% phospholipon90G, 0.04% sodium deoxycholate, and 0.29% stearylamine, and showed 84.72%, 268.13 nm, 5.89 µg/cm2/h, and 16.21 µg/cm2 /24h for Y1, Y2, Y3, and Y4, respectively.					
36841370	9	86	theme	optimum	1695:1701	arg1	gel					1713:1715	the optimum bilosomal gel	1691:1715	the optimum bilosomal gel	1691:1715	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	1	87	dep	mobility	193:200	arg1	the					179:181	the	179:181	the	179:181	Arthritis is a debilitating disease that affects the patient's mobility and quality of life.					
36841370	3	88	theme	composite	472:480	arg1	design					482:487	the Draper-Lin small composite design	451:487	the Draper-Lin small composite design based on the optimization of 4 factors	451:526	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	7	89	theme	thermal	1147:1153	arg1	analysis					1155:1162	The thermal analysis	1143:1162	The thermal analysis of OFP	1143:1169	The thermal analysis of OFP demonstrated a single broad endothermic peak for bilosomes with no detectable peak for the amorphous drug.					
36841370	9	90	theme	gel	1713:1715	arg1	application					1676:1686	once daily application	1665:1686	once daily application of the optimum bilosomal gel	1665:1715	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	2	91	theme	transdermal	338:348	arg1	treatment					350:358	the efficient transdermal treatment	324:358	the efficient transdermal treatment of arthritis	324:371	This study focused on the development and optimization of a cationic nanosized bilosomal formula for the efficient transdermal treatment of arthritis.					
36841370	9	92	theme	OFP	1497:1499	arg1	efficacy					1473:1480	the promising efficacy	1459:1480	the promising efficacy of the optimum OFP	1459:1499	The in-vivo study further proved the promising efficacy of the optimum OFP, where a complete recovery of the normal histological structure of a rat joint and normal levels of the inflammatory markers were observed within 20 days following once daily application of the optimum bilosomal gel.					
36841370	3	93	theme	skin	613:616	arg1	accumulation					618:629	skin accumulation	613:629	skin accumulation (Y4)	613:634	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
36841370	3	93	theme	skin	613:616	arg1	Y4					632:633	Y4	632:633	Y4	632:633	An optimum Fluticasone Propionate-loaded bilosomes (OFP) was developed using the Draper-Lin small composite design based on the optimization of 4 factors and evaluation of entrapment efficiency (Y1), vesicle size (Y2), skin flux (Y3), and skin accumulation (Y4).					
35240948	7	0	dep	transform	946:954	arg1	infrared					956:963	infrared	956:963	transform infrared spectroscopy and 1H-nuclear magnetic resonance	946:1010	The chemical structure of CYC-βCD was confirmed by Fourier transform infrared spectroscopy and 1H-nuclear magnetic resonance.					
35240948	8	1	theme	small-angle	1140:1150	arg1	scattering					1158:1167	small-angle X-ray scattering	1140:1167	small-angle X-ray scattering	1140:1167	The chemical characterization of CYC-βCD/GPTMS film was performed by differential scanning calorimetry, X-ray diffraction, and small-angle X-ray scattering.					
35240948	1	2	theme	rates	202:206	arg1	worldwide					208:216	all mortality rates worldwide	188:216	all mortality rates worldwide	188:216	The mortality rate of cardiovascular diseases is the highest among all mortality rates worldwide.					
35240948	6	3	dep	cyclopeptide（CYC）to	725:743	arg1	modify					745:750	modify	745:750	modify β-cyclodextrin and mixed it with γ-glycerol methoxytrimethoxysilane (GPTMS)	745:826	We used liquid-crystal cyclopeptide（CYC）to modify β-cyclodextrin and mixed it with γ-glycerol methoxytrimethoxysilane (GPTMS) to prepare CYC-β-cyclodextrin (βCD)/GPTMS film by sol-gel.					
35240948	11	4	theme	tissue	1612:1617	arg1	scaffolds					1631:1639	tissue engineering scaffolds	1612:1639	tissue engineering scaffolds	1612:1639	These results indicated the potential applications of CYC-βCD/GPTMS film in tissue engineering scaffolds.					
35240948	9	5	theme	liquid-crystal	1368:1381	arg1	structure					1383:1391	the liquid-crystal structure	1364:1391	the liquid-crystal structure of the film	1364:1403	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	9	6	dep	morphology	1182:1191	arg1	The					1170:1172	The	1170:1172	The	1170:1172	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	7	7	theme	1H-nuclear	982:991	arg1	resonance					1002:1010	1H-nuclear magnetic resonance	982:1010	1H-nuclear magnetic resonance	982:1010	The chemical structure of CYC-βCD was confirmed by Fourier transform infrared spectroscopy and 1H-nuclear magnetic resonance.					
35240948	10	8	contain	had	1462:1464	arg2	cytocompatibility					1471:1487	good cytocompatibility	1466:1487	good cytocompatibility	1466:1487	Cell culture experiments showed that CYC-βCD/GPTMS film had good cytocompatibility and induced growth and proliferation of cells.					
35240948	10	8	contain	had	1462:1464	arg1	film					1457:1460	CYC-βCD/GPTMS film	1443:1460	CYC-βCD/GPTMS film	1443:1460	Cell culture experiments showed that CYC-βCD/GPTMS film had good cytocompatibility and induced growth and proliferation of cells.					
35240948	10	9	theme	culture	1411:1417	arg1	experiments					1419:1429	Cell culture experiments	1406:1429	Cell culture experiments	1406:1429	Cell culture experiments showed that CYC-βCD/GPTMS film had good cytocompatibility and induced growth and proliferation of cells.					
35240948	4	10	theme	many	487:490	arg1	stenosis					526:533	stenosis	526:533	stenosis	526:533	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	4	10	theme	many	487:490	arg1	challenges					492:501	many challenges	487:501	many challenges	487:501	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	4	10	theme	many	487:490	arg1	thrombosis					514:523	thrombosis	514:523	thrombosis	514:523	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	4	10	theme	many	487:490	arg1	infection					540:548	infection	540:548	infection	540:548	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	8	11	theme	chemical	1017:1024	arg1	characterization					1026:1041	The chemical characterization	1013:1041	The chemical characterization of CYC-βCD/GPTMS film	1013:1063	The chemical characterization of CYC-βCD/GPTMS film was performed by differential scanning calorimetry, X-ray diffraction, and small-angle X-ray scattering.					
35240948	10	12	theme	Cell	1406:1409	arg1	experiments					1419:1429	Cell culture experiments	1406:1429	Cell culture experiments	1406:1429	Cell culture experiments showed that CYC-βCD/GPTMS film had good cytocompatibility and induced growth and proliferation of cells.					
35240948	8	13	theme	X-ray	1117:1121	arg1	diffraction					1123:1133	X-ray diffraction	1117:1133	X-ray diffraction	1117:1133	The chemical characterization of CYC-βCD/GPTMS film was performed by differential scanning calorimetry, X-ray diffraction, and small-angle X-ray scattering.					
35240948	0	14	theme	Cyclopeptide-β-cyclodextrin/γ-glycerol	0:37	arg1	film					63:66	Cyclopeptide-β-cyclodextrin/γ-glycerol methoxytrimethoxysilane film	0:66	Cyclopeptide-β-cyclodextrin/γ-glycerol methoxytrimethoxysilane film for potential vascular tissue engineering	0:108	Cyclopeptide-β-cyclodextrin/γ-glycerol methoxytrimethoxysilane film for potential vascular tissue engineering scaffolds.					
35240948	6	15	theme	/GPTMS	863:868	arg1	film					870:873	CYC-β-cyclodextrin (βCD)/GPTMS film	839:873	CYC-β-cyclodextrin (βCD)/GPTMS film by sol-gel	839:884	We used liquid-crystal cyclopeptide（CYC）to modify β-cyclodextrin and mixed it with γ-glycerol methoxytrimethoxysilane (GPTMS) to prepare CYC-β-cyclodextrin (βCD)/GPTMS film by sol-gel.					
35240948	9	16	theme	atomic	1293:1298	arg1	microscopy					1306:1315	atomic force microscopy	1293:1315	atomic force microscopy	1293:1315	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	8	17	theme	CYC-βCD/GPTMS	1046:1058	arg1	film					1060:1063	CYC-βCD/GPTMS film	1046:1063	CYC-βCD/GPTMS film	1046:1063	The chemical characterization of CYC-βCD/GPTMS film was performed by differential scanning calorimetry, X-ray diffraction, and small-angle X-ray scattering.					
35240948	9	18	theme	polarizing	1335:1344	arg1	microscopy					1346:1355	polarizing microscopy	1335:1355	polarizing microscopy	1335:1355	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	5	19	theme	compositional	605:617	arg1	separation					690:699	appropriate phase separation	672:699	appropriate phase separation	672:699	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	5	19	theme	compositional	605:617	arg1	porosity					658:665	porosity	658:665	porosity	658:665	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	5	19	theme	compositional	605:617	arg1	requirements					619:630	structural and compositional requirements	590:630	structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation	590:699	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	5	19	theme	compositional	605:617	arg1	biocompatibility					640:655	biocompatibility	640:655	biocompatibility	640:655	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	9	20	theme	separation	1203:1212	arg1	microstructure					1214:1227	phase separation microstructure	1197:1227	phase separation microstructure	1197:1227	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	3	21	theme	new	343:345	arg1	avenues					347:353	new avenues	343:353	new avenues for the treatment of cardiovascular diseases	343:398	Tissue engineering provides new avenues for the treatment of cardiovascular diseases.					
35240948	4	22	theme	tissue-engineered	454:470	arg1	scaffolds					472:480	the current small-diameter (<6 mm) vascular tissue-engineered scaffolds	410:480	the current small-diameter (<6 mm) vascular tissue-engineered scaffolds	410:480	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	1	23	theme	mortality	125:133	arg1	highest					174:180	highest	174:180	highest	174:180	The mortality rate of cardiovascular diseases is the highest among all mortality rates worldwide.					
35240948	1	23	theme	mortality	125:133	arg1	rate					135:138	The mortality rate	121:138	The mortality rate of cardiovascular diseases	121:165	The mortality rate of cardiovascular diseases is the highest among all mortality rates worldwide.					
35240948	6	24	theme	βCD	859:861	arg1	film					870:873	CYC-β-cyclodextrin (βCD)/GPTMS film	839:873	CYC-β-cyclodextrin (βCD)/GPTMS film by sol-gel	839:884	We used liquid-crystal cyclopeptide（CYC）to modify β-cyclodextrin and mixed it with γ-glycerol methoxytrimethoxysilane (GPTMS) to prepare CYC-β-cyclodextrin (βCD)/GPTMS film by sol-gel.					
35240948	9	25	theme	surface	1174:1180	arg1	morphology					1182:1191	surface morphology	1174:1191	surface morphology	1174:1191	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	0	26	theme	methoxytrimethoxysilane	39:61	arg1	film					63:66	Cyclopeptide-β-cyclodextrin/γ-glycerol methoxytrimethoxysilane film	0:66	Cyclopeptide-β-cyclodextrin/γ-glycerol methoxytrimethoxysilane film for potential vascular tissue engineering	0:108	Cyclopeptide-β-cyclodextrin/γ-glycerol methoxytrimethoxysilane film for potential vascular tissue engineering scaffolds.					
35240948	3	27	theme	Tissue	315:320	arg1	engineering					322:332	Tissue engineering	315:332	Tissue engineering	315:332	Tissue engineering provides new avenues for the treatment of cardiovascular diseases.					
35240948	4	28	theme	vascular	445:452	arg1	scaffolds					472:480	the current small-diameter (<6 mm) vascular tissue-engineered scaffolds	410:480	the current small-diameter (<6 mm) vascular tissue-engineered scaffolds	410:480	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	8	29	theme	differential	1082:1093	arg1	calorimetry					1104:1114	differential scanning calorimetry	1082:1114	differential scanning calorimetry	1082:1114	The chemical characterization of CYC-βCD/GPTMS film was performed by differential scanning calorimetry, X-ray diffraction, and small-angle X-ray scattering.					
35240948	8	30	theme	film	1060:1063	arg1	characterization					1026:1041	The chemical characterization	1013:1041	The chemical characterization of CYC-βCD/GPTMS film	1013:1063	The chemical characterization of CYC-βCD/GPTMS film was performed by differential scanning calorimetry, X-ray diffraction, and small-angle X-ray scattering.					
35240948	11	31	theme	CYC-βCD/GPTMS	1590:1602	arg1	film					1604:1607	CYC-βCD/GPTMS film	1590:1607	CYC-βCD/GPTMS film	1590:1607	These results indicated the potential applications of CYC-βCD/GPTMS film in tissue engineering scaffolds.					
35240948	4	32	contain	have	482:485	arg1	scaffolds					472:480	the current small-diameter (<6 mm) vascular tissue-engineered scaffolds	410:480	the current small-diameter (<6 mm) vascular tissue-engineered scaffolds	410:480	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	4	32	contain	have	482:485	arg2	thrombosis					514:523	thrombosis	514:523	thrombosis	514:523	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	4	32	contain	have	482:485	arg2	challenges					492:501	many challenges	487:501	many challenges	487:501	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	4	32	contain	have	482:485	arg2	infection					540:548	infection	540:548	infection	540:548	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	4	32	contain	have	482:485	arg2	stenosis					526:533	stenosis	526:533	stenosis	526:533	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	9	33	theme	film	1236:1239	arg1	microstructure					1214:1227	phase separation microstructure	1197:1227	phase separation microstructure	1197:1227	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	9	33	theme	film	1236:1239	arg1	morphology					1182:1191	surface morphology	1174:1191	surface morphology	1174:1191	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	5	34	contain	have	585:588	arg2	separation					690:699	appropriate phase separation	672:699	appropriate phase separation	672:699	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	5	34	contain	have	585:588	arg2	requirements					619:630	structural and compositional requirements	590:630	structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation	590:699	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	5	34	contain	have	585:588	arg2	porosity					658:665	porosity	658:665	porosity	658:665	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	5	34	contain	have	585:588	arg1	scaffolds					575:583	Small-diameter vascular scaffolds	551:583	Small-diameter vascular scaffolds	551:583	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	5	34	contain	have	585:588	arg2	biocompatibility					640:655	biocompatibility	640:655	biocompatibility	640:655	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	5	35	theme	vascular	566:573	arg1	scaffolds					575:583	Small-diameter vascular scaffolds	551:583	Small-diameter vascular scaffolds	551:583	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	10	36	theme	CYC-βCD/GPTMS	1443:1455	arg1	film					1457:1460	CYC-βCD/GPTMS film	1443:1460	CYC-βCD/GPTMS film	1443:1460	Cell culture experiments showed that CYC-βCD/GPTMS film had good cytocompatibility and induced growth and proliferation of cells.					
35240948	7	37	theme	magnetic	993:1000	arg1	resonance					1002:1010	1H-nuclear magnetic resonance	982:1010	1H-nuclear magnetic resonance	982:1010	The chemical structure of CYC-βCD was confirmed by Fourier transform infrared spectroscopy and 1H-nuclear magnetic resonance.					
35240948	1	38	theme	cardiovascular	143:156	arg1	diseases					158:165	cardiovascular diseases	143:165	cardiovascular diseases	143:165	The mortality rate of cardiovascular diseases is the highest among all mortality rates worldwide.					
35240948	6	39	theme	liquid-crystal	710:723	arg1	cyclopeptide（CYC）to					725:743	liquid-crystal cyclopeptide（CYC）to	710:743	liquid-crystal cyclopeptide（CYC）to modify β-cyclodextrin and mixed it with γ-glycerol methoxytrimethoxysilane (GPTMS)	710:826	We used liquid-crystal cyclopeptide（CYC）to modify β-cyclodextrin and mixed it with γ-glycerol methoxytrimethoxysilane (GPTMS) to prepare CYC-β-cyclodextrin (βCD)/GPTMS film by sol-gel.					
35240948	1	40	theme	diseases	158:165	arg1	highest					174:180	highest	174:180	highest	174:180	The mortality rate of cardiovascular diseases is the highest among all mortality rates worldwide.					
35240948	1	40	theme	diseases	158:165	arg1	rate					135:138	The mortality rate	121:138	The mortality rate of cardiovascular diseases	121:165	The mortality rate of cardiovascular diseases is the highest among all mortality rates worldwide.					
35240948	8	41	theme	scanning	1095:1102	arg1	calorimetry					1104:1114	differential scanning calorimetry	1082:1114	differential scanning calorimetry	1082:1114	The chemical characterization of CYC-βCD/GPTMS film was performed by differential scanning calorimetry, X-ray diffraction, and small-angle X-ray scattering.					
35240948	6	42	theme	CYC-β-cyclodextrin	839:856	arg1	film					870:873	CYC-β-cyclodextrin (βCD)/GPTMS film	839:873	CYC-β-cyclodextrin (βCD)/GPTMS film by sol-gel	839:884	We used liquid-crystal cyclopeptide（CYC）to modify β-cyclodextrin and mixed it with γ-glycerol methoxytrimethoxysilane (GPTMS) to prepare CYC-β-cyclodextrin (βCD)/GPTMS film by sol-gel.					
35240948	0	43	theme	vascular	82:89	arg1	engineering					98:108	potential vascular tissue engineering	72:108	potential vascular tissue engineering	72:108	Cyclopeptide-β-cyclodextrin/γ-glycerol methoxytrimethoxysilane film for potential vascular tissue engineering scaffolds.					
35240948	10	44	theme	good	1466:1469	arg1	cytocompatibility					1471:1487	good cytocompatibility	1466:1487	good cytocompatibility	1466:1487	Cell culture experiments showed that CYC-βCD/GPTMS film had good cytocompatibility and induced growth and proliferation of cells.					
35240948	5	45	theme	structural	590:599	arg1	separation					690:699	appropriate phase separation	672:699	appropriate phase separation	672:699	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	5	45	theme	structural	590:599	arg1	porosity					658:665	porosity	658:665	porosity	658:665	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	5	45	theme	structural	590:599	arg1	requirements					619:630	structural and compositional requirements	590:630	structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation	590:699	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	5	45	theme	structural	590:599	arg1	biocompatibility					640:655	biocompatibility	640:655	biocompatibility	640:655	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	0	46	theme	potential	72:80	arg1	engineering					98:108	potential vascular tissue engineering	72:108	potential vascular tissue engineering	72:108	Cyclopeptide-β-cyclodextrin/γ-glycerol methoxytrimethoxysilane film for potential vascular tissue engineering scaffolds.					
35240948	11	47	theme	film	1604:1607	arg1	applications					1574:1585	the potential applications	1560:1585	the potential applications of CYC-βCD/GPTMS film in tissue engineering scaffolds	1560:1639	These results indicated the potential applications of CYC-βCD/GPTMS film in tissue engineering scaffolds.					
35240948	9	48	theme	force	1300:1304	arg1	microscopy					1306:1315	atomic force microscopy	1293:1315	atomic force microscopy	1293:1315	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	9	49	theme	phase	1197:1201	arg1	microstructure					1214:1227	phase separation microstructure	1197:1227	phase separation microstructure	1197:1227	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	3	50	theme	diseases	391:398	arg1	treatment					363:371	the treatment	359:371	the treatment of cardiovascular diseases	359:398	Tissue engineering provides new avenues for the treatment of cardiovascular diseases.					
35240948	6	51	used	used	705:708	arg2	We					702:703	We	702:703	We	702:703	We used liquid-crystal cyclopeptide（CYC）to modify β-cyclodextrin and mixed it with γ-glycerol methoxytrimethoxysilane (GPTMS) to prepare CYC-β-cyclodextrin (βCD)/GPTMS film by sol-gel.					
35240948	4	52	theme	small-diameter	422:435	arg1	scaffolds					472:480	the current small-diameter (<6 mm) vascular tissue-engineered scaffolds	410:480	the current small-diameter (<6 mm) vascular tissue-engineered scaffolds	410:480	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	10	53	theme	cells	1529:1533	arg1	proliferation					1512:1524	proliferation	1512:1524	proliferation	1512:1524	Cell culture experiments showed that CYC-βCD/GPTMS film had good cytocompatibility and induced growth and proliferation of cells.					
35240948	10	53	theme	cells	1529:1533	arg1	growth					1501:1506	growth	1501:1506	growth	1501:1506	Cell culture experiments showed that CYC-βCD/GPTMS film had good cytocompatibility and induced growth and proliferation of cells.					
35240948	0	54	theme	tissue	91:96	arg1	engineering					98:108	potential vascular tissue engineering	72:108	potential vascular tissue engineering	72:108	Cyclopeptide-β-cyclodextrin/γ-glycerol methoxytrimethoxysilane film for potential vascular tissue engineering scaffolds.					
35240948	7	55	theme	CYC-βCD	913:919	arg1	structure					900:908	The chemical structure	887:908	The chemical structure of CYC-βCD	887:919	The chemical structure of CYC-βCD was confirmed by Fourier transform infrared spectroscopy and 1H-nuclear magnetic resonance.					
35240948	11	56	from	applications	1574:1585	arg1	scaffolds					1631:1639	tissue engineering scaffolds	1612:1639	tissue engineering scaffolds	1612:1639	These results indicated the potential applications of CYC-βCD/GPTMS film in tissue engineering scaffolds.					
35240948	2	57	theme	rejection	278:286	arg1	reaction					288:295	rejection reaction	278:295	rejection reaction	278:295	Allotransplantation and autotransplantation are limited by rejection reaction and availability.					
35240948	5	58	theme	appropriate	672:682	arg1	separation					690:699	appropriate phase separation	672:699	appropriate phase separation	672:699	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	5	59	theme	phase	684:688	arg1	separation					690:699	appropriate phase separation	672:699	appropriate phase separation	672:699	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	4	60	theme	<6 mm	438:442	arg1	scaffolds					472:480	the current small-diameter (<6 mm) vascular tissue-engineered scaffolds	410:480	the current small-diameter (<6 mm) vascular tissue-engineered scaffolds	410:480	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	8	61	theme	X-ray	1152:1156	arg1	scattering					1158:1167	small-angle X-ray scattering	1140:1167	small-angle X-ray scattering	1140:1167	The chemical characterization of CYC-βCD/GPTMS film was performed by differential scanning calorimetry, X-ray diffraction, and small-angle X-ray scattering.					
35240948	3	62	theme	cardiovascular	376:389	arg1	diseases					391:398	cardiovascular diseases	376:398	cardiovascular diseases	376:398	Tissue engineering provides new avenues for the treatment of cardiovascular diseases.					
35240948	9	63	theme	microscopy	1346:1355	arg1	image					1326:1330	the image	1322:1330	the image of polarizing microscopy	1322:1355	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	9	64	theme	film	1400:1403	arg1	structure					1383:1391	the liquid-crystal structure	1364:1391	the liquid-crystal structure of the film	1364:1403	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	11	65	theme	potential	1564:1572	arg1	applications					1574:1585	the potential applications	1560:1585	the potential applications of CYC-βCD/GPTMS film in tissue engineering scaffolds	1560:1639	These results indicated the potential applications of CYC-βCD/GPTMS film in tissue engineering scaffolds.					
35240948	11	66	theme	engineering	1619:1629	arg1	scaffolds					1631:1639	tissue engineering scaffolds	1612:1639	tissue engineering scaffolds	1612:1639	These results indicated the potential applications of CYC-βCD/GPTMS film in tissue engineering scaffolds.					
35240948	9	67	theme	scanning	1260:1267	arg1	microscopy					1278:1287	scanning electron microscopy	1260:1287	scanning electron microscopy	1260:1287	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	5	68	theme	Small-diameter	551:564	arg1	scaffolds					575:583	Small-diameter vascular scaffolds	551:583	Small-diameter vascular scaffolds	551:583	Small-diameter vascular scaffolds have structural and compositional requirements such as biocompatibility, porosity, and appropriate phase separation.					
35240948	7	69	dep	Fourier	938:944	arg1	transform					946:954	transform	946:954	transform infrared spectroscopy and 1H-nuclear magnetic resonance	946:1010	The chemical structure of CYC-βCD was confirmed by Fourier transform infrared spectroscopy and 1H-nuclear magnetic resonance.					
35240948	6	70	theme	γ-glycerol	785:794	arg1	methoxytrimethoxysilane					796:818	γ-glycerol methoxytrimethoxysilane	785:818	γ-glycerol methoxytrimethoxysilane (GPTMS)	785:826	We used liquid-crystal cyclopeptide（CYC）to modify β-cyclodextrin and mixed it with γ-glycerol methoxytrimethoxysilane (GPTMS) to prepare CYC-β-cyclodextrin (βCD)/GPTMS film by sol-gel.					
35240948	6	70	theme	γ-glycerol	785:794	arg1	GPTMS					821:825	GPTMS	821:825	GPTMS	821:825	We used liquid-crystal cyclopeptide（CYC）to modify β-cyclodextrin and mixed it with γ-glycerol methoxytrimethoxysilane (GPTMS) to prepare CYC-β-cyclodextrin (βCD)/GPTMS film by sol-gel.					
35240948	4	71	theme	current	414:420	arg1	scaffolds					472:480	the current small-diameter (<6 mm) vascular tissue-engineered scaffolds	410:480	the current small-diameter (<6 mm) vascular tissue-engineered scaffolds	410:480	However, the current small-diameter (<6 mm) vascular tissue-engineered scaffolds have many challenges, including thrombosis, stenosis, and infection.					
35240948	1	72	theme	mortality	192:200	arg1	rates					202:206	all mortality rates	188:206	all mortality rates worldwide	188:216	The mortality rate of cardiovascular diseases is the highest among all mortality rates worldwide.					
35240948	9	73	theme	electron	1269:1276	arg1	microscopy					1278:1287	scanning electron microscopy	1260:1287	scanning electron microscopy	1260:1287	The surface morphology and phase separation microstructure of the film were determined by scanning electron microscopy and atomic force microscopy, and the image of polarizing microscopy showed the liquid-crystal structure of the film.					
35240948	7	74	theme	chemical	891:898	arg1	structure					900:908	The chemical structure	887:908	The chemical structure of CYC-βCD	887:919	The chemical structure of CYC-βCD was confirmed by Fourier transform infrared spectroscopy and 1H-nuclear magnetic resonance.					
35821317	14	0	theme	@	2071:2071	arg1	CA-PES					2073:2078	GO-CuO @ CA-PES	2064:2078	GO-CuO @ CA-PES	2064:2078	The fabricated TFN membrane material (GO-CuO @ CA-PES) can be recommended for water treatment applications.					
35821317	14	0	theme	@	2071:2071	arg1	material					2054:2061	The fabricated TFN membrane material	2026:2061	The fabricated TFN membrane material (GO-CuO @ CA-PES)	2026:2079	The fabricated TFN membrane material (GO-CuO @ CA-PES) can be recommended for water treatment applications.					
35821317	6	1	theme	blue	1150:1153	arg1	methylene					1140:1148	methylene blue	1140:1153	four typical water-soluble organic dyes methylene blue (MB)	1100:1158	Dye adsorption experiments were performed with four typical water-soluble organic dyes methylene blue (MB), rhodamine blue (Rh.					
35821317	6	1	theme	blue	1150:1153	arg1	MB					1156:1157	MB	1156:1157	MB	1156:1157	Dye adsorption experiments were performed with four typical water-soluble organic dyes methylene blue (MB), rhodamine blue (Rh.					
35821317	10	2	theme	TFN	1410:1412	arg1	material					1459:1466	an effective adsorbent material	1436:1466	an effective adsorbent material for cationic dye molecules	1436:1493	As a result, the fabricated TFN material proves to be an effective adsorbent material for cationic dye molecules.					
35821317	10	2	theme	TFN	1410:1412	arg1	material					1414:1421	the fabricated TFN material	1395:1421	the fabricated TFN material	1395:1421	As a result, the fabricated TFN material proves to be an effective adsorbent material for cationic dye molecules.					
35821317	2	3	theme	treated	276:282	arg1	effluent					292:299	treated textile effluent	276:299	treated textile effluent that exceeds regulatory limitations	276:335	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	11	4	from	decrease	1637:1644	arg1	concentrations					1649:1662	concentrations	1649:1662	concentrations	1649:1662	Also, when the fabricated material was tested with textile industry effluent sample, all physio-chemical properties exhibited a considerable decrease in concentrations when compared to the real textile effluent concentration.					
35821317	4	5	theme	phase	643:647	arg1	process					659:665	phase inversion process	643:665	phase inversion process	643:665	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	14	6	theme	membrane	2045:2052	arg1	CA-PES					2073:2078	GO-CuO @ CA-PES	2064:2078	GO-CuO @ CA-PES	2064:2078	The fabricated TFN membrane material (GO-CuO @ CA-PES) can be recommended for water treatment applications.					
35821317	14	6	theme	membrane	2045:2052	arg1	material					2054:2061	The fabricated TFN membrane material	2026:2061	The fabricated TFN membrane material (GO-CuO @ CA-PES)	2026:2079	The fabricated TFN membrane material (GO-CuO @ CA-PES) can be recommended for water treatment applications.					
35821317	2	7	theme	color	267:271	arg1	amount					250:255	the amount	246:255	the amount of excess color in treated textile effluent that exceeds regulatory limitations	246:335	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	2	7	theme	color	267:271	arg1	color					267:271	excess color	260:271	excess color	260:271	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	2	7	theme	color	267:271	arg1	concern					360:366	a major concern	352:366	a major concern	352:366	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	6	8	theme	water-soluble	1113:1125	arg1	dyes					1135:1138	four typical water-soluble organic dyes methylene blue (MB)	1100:1158	four typical water-soluble organic dyes methylene blue (MB)	1100:1158	Dye adsorption experiments were performed with four typical water-soluble organic dyes methylene blue (MB), rhodamine blue (Rh.					
35821317	14	9	theme	fabricated	2030:2039	arg1	CA-PES					2073:2078	GO-CuO @ CA-PES	2064:2078	GO-CuO @ CA-PES	2064:2078	The fabricated TFN membrane material (GO-CuO @ CA-PES) can be recommended for water treatment applications.					
35821317	14	9	theme	fabricated	2030:2039	arg1	material					2054:2061	The fabricated TFN membrane material	2026:2061	The fabricated TFN membrane material (GO-CuO @ CA-PES)	2026:2079	The fabricated TFN membrane material (GO-CuO @ CA-PES) can be recommended for water treatment applications.					
35821317	1	10	theme	harmful	198:204	arg1	effluents					210:218	harmful dye effluents	198:218	harmful dye effluents	198:218	Textile industries are one of the leading environmental pollutants by releasing harmful dye effluents.					
35821317	2	11	from	concern	360:366	arg1	distrts					237:243	many textile distrts	224:243	many textile distrts	224:243	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	6	12	dep	dyes	1135:1138	arg1	methylene					1140:1148	methylene blue	1140:1153	four typical water-soluble organic dyes methylene blue (MB)	1100:1158	Dye adsorption experiments were performed with four typical water-soluble organic dyes methylene blue (MB), rhodamine blue (Rh.					
35821317	6	12	dep	dyes	1135:1138	arg1	MB					1156:1157	MB	1156:1157	MB	1156:1157	Dye adsorption experiments were performed with four typical water-soluble organic dyes methylene blue (MB), rhodamine blue (Rh.					
35821317	12	13	theme	Tropical	1811:1818	arg1	roots					1829:1833	Tropical amaranth roots	1811:1833	Tropical amaranth roots	1811:1833	The treated effluents permitted for a relatively greater growth and germination index of Tropical amaranth roots than the textile effluent, this demonstrates that phytotoxicity testing was also successful.					
35821317	5	14	theme	infra-red	839:847	arg1	FT-IR					863:867	FT-IR	863:867	FT-IR	863:867	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	5	14	theme	infra-red	839:847	arg1	spectroscopy					849:860	infra-red spectroscopy	839:860	infra-red spectroscopy (FT-IR)	839:868	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	12	15	theme	roots	1829:1833	arg1	growth					1779:1784	a relatively greater growth	1758:1784	a relatively greater growth	1758:1784	The treated effluents permitted for a relatively greater growth and germination index of Tropical amaranth roots than the textile effluent, this demonstrates that phytotoxicity testing was also successful.					
35821317	12	15	theme	roots	1829:1833	arg1	index					1802:1806	germination index	1790:1806	germination index	1790:1806	The treated effluents permitted for a relatively greater growth and germination index of Tropical amaranth roots than the textile effluent, this demonstrates that phytotoxicity testing was also successful.					
35821317	6	16	theme	blue	1171:1174	arg1	dyes					1135:1138	four typical water-soluble organic dyes methylene blue (MB)	1100:1158	four typical water-soluble organic dyes methylene blue (MB)	1100:1158	Dye adsorption experiments were performed with four typical water-soluble organic dyes methylene blue (MB), rhodamine blue (Rh.					
35821317	10	17	theme	effective	1439:1447	arg1	material					1459:1466	an effective adsorbent material	1436:1466	an effective adsorbent material for cationic dye molecules	1436:1493	As a result, the fabricated TFN material proves to be an effective adsorbent material for cationic dye molecules.					
35821317	10	17	theme	effective	1439:1447	arg1	material					1414:1421	the fabricated TFN material	1395:1421	the fabricated TFN material	1395:1421	As a result, the fabricated TFN material proves to be an effective adsorbent material for cationic dye molecules.					
35821317	5	18	theme	gravimetric	991:1001	arg1	TGA					1013:1015	TGA	1013:1015	TGA	1013:1015	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	5	18	theme	gravimetric	991:1001	arg1	analysis					1003:1010	thermo gravimetric analysis	984:1010	thermo gravimetric analysis (TGA)	984:1016	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	2	19	theme	many	224:227	arg1	distrts					237:243	many textile distrts	224:243	many textile distrts	224:243	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	0	20	from	wastewater	106:115	arg1	removal					77:83	adsorptive removal	66:83	adsorptive removal of organic dyes from wastewater	66:115	GO-CuO nanocomposites assimilated into CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater.					
35821317	0	20	from	wastewater	106:115	arg1	dyes					96:99	organic dyes	88:99	organic dyes from wastewater	88:115	GO-CuO nanocomposites assimilated into CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater.					
35821317	9	21	theme	MO	1358:1359	arg1	%					1366:1366	MO 58.82%	1358:1366	MO 58.82%	1358:1366	B 89.39%, CR 68.39%, and MO 58.82% respectively.					
35821317	0	22	theme	adsorptive	66:75	arg1	removal					77:83	adsorptive removal	66:83	adsorptive removal of organic dyes from wastewater	66:115	GO-CuO nanocomposites assimilated into CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater.					
35821317	13	23	theme	effective	1937:1945	arg1	temperature					1947:1957	The most effective temperature	1928:1957	The most effective temperature	1928:1957	The most effective temperature, concentration and pH were found to be 273 K, 1 × 10-5 M and pH 9.					
35821317	13	23	theme	effective	1937:1945	arg1	273 K					1998:2002	273 K	1998:2002	273 K	1998:2002	The most effective temperature, concentration and pH were found to be 273 K, 1 × 10-5 M and pH 9.					
35821317	5	24	theme	X-ray	871:875	arg1	diffractometer					877:890	X-ray diffractometer	871:890	X-ray diffractometer (XRD)	871:896	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	5	24	theme	X-ray	871:875	arg1	XRD					893:895	XRD	893:895	XRD	893:895	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	4	25	theme	graphene	493:500	arg1	nanomaterial					530:541	graphene oxide-copper oxide (GO-CuO) nanomaterial	493:541	graphene oxide-copper oxide (GO-CuO) nanomaterial	493:541	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	4	26	theme	film	685:688	arg1	TFN					705:707	TFN	705:707	TFN	705:707	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	4	26	theme	film	685:688	arg1	nanocomposite					690:702	thin film nanocomposite	680:702	thin film nanocomposite (TFN) membrane	680:717	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	1	27	theme	environmental	160:172	arg1	pollutants					174:183	the leading environmental pollutants	148:183	the leading environmental pollutants	148:183	Textile industries are one of the leading environmental pollutants by releasing harmful dye effluents.					
35821317	4	28	theme	oxide	515:519	arg1	nanomaterial					530:541	graphene oxide-copper oxide (GO-CuO) nanomaterial	493:541	graphene oxide-copper oxide (GO-CuO) nanomaterial	493:541	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	4	29	theme	pollutant	738:746	arg1	removal					723:729	removal	723:729	removal of dye pollutant	723:746	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	0	30	theme	dyes	96:99	arg1	removal					77:83	adsorptive removal	66:83	adsorptive removal of organic dyes from wastewater	66:115	GO-CuO nanocomposites assimilated into CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater.					
35821317	5	31	theme	strength	1034:1041	arg1	analysis					1043:1050	mechanical strength analysis	1023:1050	mechanical strength analysis	1023:1050	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	12	32	theme	germination	1790:1800	arg1	index					1802:1806	germination index	1790:1806	germination index	1790:1806	The treated effluents permitted for a relatively greater growth and germination index of Tropical amaranth roots than the textile effluent, this demonstrates that phytotoxicity testing was also successful.					
35821317	5	33	theme	field	899:903	arg1	FE-SEM					944:949	FE-SEM	944:949	FE-SEM	944:949	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	5	33	theme	field	899:903	arg1	microscopy					932:941	field emission scanning electron microscopy	899:941	field emission scanning electron microscopy (FE-SEM)	899:950	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	0	34	from	membrane	54:61	arg1	removal					77:83	adsorptive removal	66:83	adsorptive removal of organic dyes from wastewater	66:115	GO-CuO nanocomposites assimilated into CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater.					
35821317	0	35	theme	polymer	46:52	arg1	membrane					54:61	CA-PES polymer membrane	39:61	CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater	39:115	GO-CuO nanocomposites assimilated into CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater.					
35821317	14	36	theme	treatment	2110:2118	arg1	applications					2120:2131	water treatment applications	2104:2131	water treatment applications	2104:2131	The fabricated TFN membrane material (GO-CuO @ CA-PES) can be recommended for water treatment applications.					
35821317	10	37	theme	dye	1481:1483	arg1	molecules					1485:1493	cationic dye molecules	1472:1493	cationic dye molecules	1472:1493	As a result, the fabricated TFN material proves to be an effective adsorbent material for cationic dye molecules.					
35821317	5	38	theme	materials	795:803	arg1	properties					768:777	The physiochemical properties	749:777	The physiochemical properties of prepared TFN materials	749:803	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	5	39	theme	scanning	914:921	arg1	FE-SEM					944:949	FE-SEM	944:949	FE-SEM	944:949	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	5	39	theme	scanning	914:921	arg1	microscopy					932:941	field emission scanning electron microscopy	899:941	field emission scanning electron microscopy (FE-SEM)	899:950	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	2	40	from	amount	250:255	arg1	effluent					292:299	treated textile effluent	276:299	treated textile effluent that exceeds regulatory limitations	276:335	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	8	41	theme	membrane	1277:1284	arg1	effectiveness					1300:1312	the composite membrane final removal effectiveness	1263:1312	the composite membrane final removal effectiveness for MB 92.42%	1263:1326	After reaching adsorption equilibrium, the composite membrane final removal effectiveness for MB 92.42%, Rh.					
35821317	8	41	theme	membrane	1277:1284	arg1	equilibrium					1250:1260	adsorption equilibrium	1239:1260	adsorption equilibrium	1239:1260	After reaching adsorption equilibrium, the composite membrane final removal effectiveness for MB 92.42%, Rh.					
35821317	3	42	theme	polymer	410:416	arg1	material					418:425	polymer material	410:425	polymer material	410:425	The combining usage of nanomaterials and polymer material to solve these issues using various techniques.					
35821317	8	43	theme	removal	1292:1298	arg1	effectiveness					1300:1312	the composite membrane final removal effectiveness	1263:1312	the composite membrane final removal effectiveness for MB 92.42%	1263:1326	After reaching adsorption equilibrium, the composite membrane final removal effectiveness for MB 92.42%, Rh.					
35821317	8	43	theme	removal	1292:1298	arg1	equilibrium					1250:1260	adsorption equilibrium	1239:1260	adsorption equilibrium	1239:1260	After reaching adsorption equilibrium, the composite membrane final removal effectiveness for MB 92.42%, Rh.					
35821317	0	44	theme	CA-PES	39:44	arg1	membrane					54:61	CA-PES polymer membrane	39:61	CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater	39:115	GO-CuO nanocomposites assimilated into CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater.					
35821317	3	45	theme	nanomaterials	392:404	arg1	usage					383:387	The combining usage	369:387	The combining usage of nanomaterials and polymer material	369:425	The combining usage of nanomaterials and polymer material to solve these issues using various techniques.					
35821317	4	46	theme	GO-CuO	522:527	arg1	nanomaterial					530:541	graphene oxide-copper oxide (GO-CuO) nanomaterial	493:541	graphene oxide-copper oxide (GO-CuO) nanomaterial	493:541	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	5	47	theme	prepared	782:789	arg1	materials					795:803	prepared TFN materials	782:803	prepared TFN materials	782:803	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	5	48	theme	thermo	984:989	arg1	TGA					1013:1015	TGA	1013:1015	TGA	1013:1015	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	5	48	theme	thermo	984:989	arg1	analysis					1003:1010	thermo gravimetric analysis	984:1010	thermo gravimetric analysis (TGA)	984:1016	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	11	49	theme	effluent	1564:1571	arg1	sample					1573:1578	textile industry effluent sample	1547:1578	textile industry effluent sample	1547:1578	Also, when the fabricated material was tested with textile industry effluent sample, all physio-chemical properties exhibited a considerable decrease in concentrations when compared to the real textile effluent concentration.					
35821317	11	50	theme	textile	1690:1696	arg1	concentration					1707:1719	the real textile effluent concentration	1681:1719	the real textile effluent concentration	1681:1719	Also, when the fabricated material was tested with textile industry effluent sample, all physio-chemical properties exhibited a considerable decrease in concentrations when compared to the real textile effluent concentration.					
35821317	5	51	theme	force	960:964	arg1	microscopy					966:975	atomic force microscopy	953:975	atomic force microscopy (AFM)	953:981	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	5	51	theme	force	960:964	arg1	AFM					978:980	AFM	978:980	AFM	978:980	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	6	52	theme	adsorption	1057:1066	arg1	experiments					1068:1078	Dye adsorption experiments	1053:1078	Dye adsorption experiments	1053:1078	Dye adsorption experiments were performed with four typical water-soluble organic dyes methylene blue (MB), rhodamine blue (Rh.					
35821317	4	53	theme	poly-ether	595:604	arg1	polymer					626:632	poly-ether sulfone (PES) blend polymer	595:632	poly-ether sulfone (PES) blend polymer	595:632	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	4	53	theme	poly-ether	595:604	arg1	cellulose-acetate					571:587	cellulose-acetate	571:587	cellulose-acetate (CA)	571:592	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	11	54	theme	textile	1547:1553	arg1	sample					1573:1578	textile industry effluent sample	1547:1578	textile industry effluent sample	1547:1578	Also, when the fabricated material was tested with textile industry effluent sample, all physio-chemical properties exhibited a considerable decrease in concentrations when compared to the real textile effluent concentration.					
35821317	6	55	dep	performed	1085:1093	arg1	Rh					1177:1178	Rh	1177:1178	Rh	1177:1178	Dye adsorption experiments were performed with four typical water-soluble organic dyes methylene blue (MB), rhodamine blue (Rh.					
35821317	12	56	theme	phytotoxicity	1885:1897	arg1	testing					1899:1905	phytotoxicity testing	1885:1905	phytotoxicity testing	1885:1905	The treated effluents permitted for a relatively greater growth and germination index of Tropical amaranth roots than the textile effluent, this demonstrates that phytotoxicity testing was also successful.					
35821317	2	57	theme	major	354:358	arg1	amount					250:255	the amount	246:255	the amount of excess color in treated textile effluent that exceeds regulatory limitations	246:335	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	2	57	theme	major	354:358	arg1	color					267:271	excess color	260:271	excess color	260:271	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	2	57	theme	major	354:358	arg1	concern					360:366	a major concern	352:366	a major concern	352:366	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	14	58	theme	GO-CuO	2064:2069	arg1	CA-PES					2073:2078	GO-CuO @ CA-PES	2064:2078	GO-CuO @ CA-PES	2064:2078	The fabricated TFN membrane material (GO-CuO @ CA-PES) can be recommended for water treatment applications.					
35821317	14	58	theme	GO-CuO	2064:2069	arg1	material					2054:2061	The fabricated TFN membrane material	2026:2061	The fabricated TFN membrane material (GO-CuO @ CA-PES)	2026:2079	The fabricated TFN membrane material (GO-CuO @ CA-PES) can be recommended for water treatment applications.					
35821317	2	59	theme	textile	284:290	arg1	effluent					292:299	treated textile effluent	276:299	treated textile effluent that exceeds regulatory limitations	276:335	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	10	60	theme	fabricated	1399:1408	arg1	material					1459:1466	an effective adsorbent material	1436:1466	an effective adsorbent material for cationic dye molecules	1436:1493	As a result, the fabricated TFN material proves to be an effective adsorbent material for cationic dye molecules.					
35821317	10	60	theme	fabricated	1399:1408	arg1	material					1414:1421	the fabricated TFN material	1395:1421	the fabricated TFN material	1395:1421	As a result, the fabricated TFN material proves to be an effective adsorbent material for cationic dye molecules.					
35821317	1	61	theme	pollutants	174:183	arg1	pollutants					174:183	the leading environmental pollutants	148:183	the leading environmental pollutants	148:183	Textile industries are one of the leading environmental pollutants by releasing harmful dye effluents.					
35821317	1	61	theme	pollutants	174:183	arg1	one					141:143	one	141:143	one	141:143	Textile industries are one of the leading environmental pollutants by releasing harmful dye effluents.					
35821317	6	62	theme	organic	1127:1133	arg1	dyes					1135:1138	four typical water-soluble organic dyes methylene blue (MB)	1100:1158	four typical water-soluble organic dyes methylene blue (MB)	1100:1158	Dye adsorption experiments were performed with four typical water-soluble organic dyes methylene blue (MB), rhodamine blue (Rh.					
35821317	14	63	theme	TFN	2041:2043	arg1	CA-PES					2073:2078	GO-CuO @ CA-PES	2064:2078	GO-CuO @ CA-PES	2064:2078	The fabricated TFN membrane material (GO-CuO @ CA-PES) can be recommended for water treatment applications.					
35821317	14	63	theme	TFN	2041:2043	arg1	material					2054:2061	The fabricated TFN membrane material	2026:2061	The fabricated TFN membrane material (GO-CuO @ CA-PES)	2026:2079	The fabricated TFN membrane material (GO-CuO @ CA-PES) can be recommended for water treatment applications.					
35821317	2	64	theme	regulatory	314:323	arg1	limitations					325:335	regulatory limitations	314:335	regulatory limitations	314:335	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	9	65	theme	B	1333:1333	arg1	%					1340:1340	B 89.39%	1333:1340	B 89.39%	1333:1340	B 89.39%, CR 68.39%, and MO 58.82% respectively.					
35821317	6	66	theme	typical	1105:1111	arg1	dyes					1135:1138	four typical water-soluble organic dyes methylene blue (MB)	1100:1158	four typical water-soluble organic dyes methylene blue (MB)	1100:1158	Dye adsorption experiments were performed with four typical water-soluble organic dyes methylene blue (MB), rhodamine blue (Rh.					
35821317	4	67	theme	inversion	649:657	arg1	process					659:665	phase inversion process	643:665	phase inversion process	643:665	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	1	68	theme	dye	206:208	arg1	effluents					210:218	harmful dye effluents	198:218	harmful dye effluents	198:218	Textile industries are one of the leading environmental pollutants by releasing harmful dye effluents.					
35821317	12	69	theme	textile	1844:1850	arg1	effluent					1852:1859	the textile effluent	1840:1859	the textile effluent	1840:1859	The treated effluents permitted for a relatively greater growth and germination index of Tropical amaranth roots than the textile effluent, this demonstrates that phytotoxicity testing was also successful.					
35821317	9	70	theme	CR	1343:1344	arg1	%					1351:1351	CR 68.39%	1343:1351	CR 68.39%	1343:1351	B 89.39%, CR 68.39%, and MO 58.82% respectively.					
35821317	0	71	from	removal	77:83	arg1	wastewater					106:115	wastewater	106:115	wastewater	106:115	GO-CuO nanocomposites assimilated into CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater.					
35821317	10	72	theme	adsorbent	1449:1457	arg1	material					1459:1466	an effective adsorbent material	1436:1466	an effective adsorbent material for cationic dye molecules	1436:1493	As a result, the fabricated TFN material proves to be an effective adsorbent material for cationic dye molecules.					
35821317	10	72	theme	adsorbent	1449:1457	arg1	material					1414:1421	the fabricated TFN material	1395:1421	the fabricated TFN material	1395:1421	As a result, the fabricated TFN material proves to be an effective adsorbent material for cationic dye molecules.					
35821317	11	73	theme	real	1685:1688	arg1	concentration					1707:1719	the real textile effluent concentration	1681:1719	the real textile effluent concentration	1681:1719	Also, when the fabricated material was tested with textile industry effluent sample, all physio-chemical properties exhibited a considerable decrease in concentrations when compared to the real textile effluent concentration.					
35821317	5	74	theme	mechanical	1023:1032	arg1	analysis					1043:1050	mechanical strength analysis	1023:1050	mechanical strength analysis	1023:1050	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	2	75	theme	textile	229:235	arg1	distrts					237:243	many textile distrts	224:243	many textile distrts	224:243	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	1	76	theme	Textile	118:124	arg1	industries					126:135	Textile industries	118:135	Textile industries	118:135	Textile industries are one of the leading environmental pollutants by releasing harmful dye effluents.					
35821317	12	77	theme	amaranth	1820:1827	arg1	roots					1829:1833	Tropical amaranth roots	1811:1833	Tropical amaranth roots	1811:1833	The treated effluents permitted for a relatively greater growth and germination index of Tropical amaranth roots than the textile effluent, this demonstrates that phytotoxicity testing was also successful.					
35821317	4	78	theme	nanocomposite	690:702	arg1	membrane					710:717	thin film nanocomposite (TFN) membrane	680:717	thin film nanocomposite (TFN) membrane	680:717	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	2	79	theme	excess	260:265	arg1	color					267:271	excess color	260:271	excess color	260:271	In many textile distrts, the amount of excess color in treated textile effluent that exceeds regulatory limitations is still being a major concern.					
35821317	11	80	theme	physio-chemical	1585:1599	arg1	properties					1601:1610	all physio-chemical properties	1581:1610	all physio-chemical properties	1581:1610	Also, when the fabricated material was tested with textile industry effluent sample, all physio-chemical properties exhibited a considerable decrease in concentrations when compared to the real textile effluent concentration.					
35821317	4	81	theme	blend	620:624	arg1	polymer					626:632	poly-ether sulfone (PES) blend polymer	595:632	poly-ether sulfone (PES) blend polymer	595:632	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	4	81	theme	blend	620:624	arg1	cellulose-acetate					571:587	cellulose-acetate	571:587	cellulose-acetate (CA)	571:592	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	4	82	theme	oxide-copper	502:513	arg1	nanomaterial					530:541	graphene oxide-copper oxide (GO-CuO) nanomaterial	493:541	graphene oxide-copper oxide (GO-CuO) nanomaterial	493:541	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	0	83	theme	organic	88:94	arg1	dyes					96:99	organic dyes	88:99	organic dyes from wastewater	88:115	GO-CuO nanocomposites assimilated into CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater.					
35821317	1	84	theme	leading	152:158	arg1	pollutants					174:183	the leading environmental pollutants	148:183	the leading environmental pollutants	148:183	Textile industries are one of the leading environmental pollutants by releasing harmful dye effluents.					
35821317	4	85	theme	thin	680:683	arg1	TFN					705:707	TFN	705:707	TFN	705:707	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	4	85	theme	thin	680:683	arg1	nanocomposite					690:702	thin film nanocomposite	680:702	thin film nanocomposite (TFN) membrane	680:717	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	11	86	theme	considerable	1624:1635	arg1	decrease					1637:1644	a considerable decrease	1622:1644	a considerable decrease in concentrations	1622:1662	Also, when the fabricated material was tested with textile industry effluent sample, all physio-chemical properties exhibited a considerable decrease in concentrations when compared to the real textile effluent concentration.					
35821317	4	87	theme	dye	734:736	arg1	pollutant					738:746	dye pollutant	734:746	dye pollutant	734:746	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	8	88	theme	adsorption	1239:1248	arg1	effectiveness					1300:1312	the composite membrane final removal effectiveness	1263:1312	the composite membrane final removal effectiveness for MB 92.42%	1263:1326	After reaching adsorption equilibrium, the composite membrane final removal effectiveness for MB 92.42%, Rh.					
35821317	8	88	theme	adsorption	1239:1248	arg1	Rh					1329:1330	Rh	1329:1330	Rh	1329:1330	After reaching adsorption equilibrium, the composite membrane final removal effectiveness for MB 92.42%, Rh.					
35821317	8	88	theme	adsorption	1239:1248	arg1	equilibrium					1250:1260	adsorption equilibrium	1239:1260	adsorption equilibrium	1239:1260	After reaching adsorption equilibrium, the composite membrane final removal effectiveness for MB 92.42%, Rh.					
35821317	5	89	theme	TFN	791:793	arg1	materials					795:803	prepared TFN materials	782:803	prepared TFN materials	782:803	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	12	90	theme	greater	1771:1777	arg1	growth					1779:1784	a relatively greater growth	1758:1784	a relatively greater growth	1758:1784	The treated effluents permitted for a relatively greater growth and germination index of Tropical amaranth roots than the textile effluent, this demonstrates that phytotoxicity testing was also successful.					
35821317	5	91	theme	emission	905:912	arg1	FE-SEM					944:949	FE-SEM	944:949	FE-SEM	944:949	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	5	91	theme	emission	905:912	arg1	microscopy					932:941	field emission scanning electron microscopy	899:941	field emission scanning electron microscopy (FE-SEM)	899:950	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	14	92	theme	water	2104:2108	arg1	applications					2120:2131	water treatment applications	2104:2131	water treatment applications	2104:2131	The fabricated TFN membrane material (GO-CuO @ CA-PES) can be recommended for water treatment applications.					
35821317	10	93	theme	cationic	1472:1479	arg1	molecules					1485:1493	cationic dye molecules	1472:1493	cationic dye molecules	1472:1493	As a result, the fabricated TFN material proves to be an effective adsorbent material for cationic dye molecules.					
35821317	12	94	theme	treated	1726:1732	arg1	effluents					1734:1742	The treated effluents	1722:1742	The treated effluents	1722:1742	The treated effluents permitted for a relatively greater growth and germination index of Tropical amaranth roots than the textile effluent, this demonstrates that phytotoxicity testing was also successful.					
35821317	5	95	theme	electron	923:930	arg1	FE-SEM					944:949	FE-SEM	944:949	FE-SEM	944:949	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	5	95	theme	electron	923:930	arg1	microscopy					932:941	field emission scanning electron microscopy	899:941	field emission scanning electron microscopy (FE-SEM)	899:950	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	3	96	theme	combining	373:381	arg1	usage					383:387	The combining usage	369:387	The combining usage of nanomaterials and polymer material	369:425	The combining usage of nanomaterials and polymer material to solve these issues using various techniques.					
35821317	8	97	theme	composite	1267:1275	arg1	effectiveness					1300:1312	the composite membrane final removal effectiveness	1263:1312	the composite membrane final removal effectiveness for MB 92.42%	1263:1326	After reaching adsorption equilibrium, the composite membrane final removal effectiveness for MB 92.42%, Rh.					
35821317	8	97	theme	composite	1267:1275	arg1	equilibrium					1250:1260	adsorption equilibrium	1239:1260	adsorption equilibrium	1239:1260	After reaching adsorption equilibrium, the composite membrane final removal effectiveness for MB 92.42%, Rh.					
35821317	5	98	theme	physiochemical	753:766	arg1	properties					768:777	The physiochemical properties	749:777	The physiochemical properties of prepared TFN materials	749:803	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	3	99	theme	material	418:425	arg1	usage					383:387	The combining usage	369:387	The combining usage of nanomaterials and polymer material	369:425	The combining usage of nanomaterials and polymer material to solve these issues using various techniques.					
35821317	11	100	theme	fabricated	1511:1520	arg1	material					1522:1529	the fabricated material	1507:1529	the fabricated material	1507:1529	Also, when the fabricated material was tested with textile industry effluent sample, all physio-chemical properties exhibited a considerable decrease in concentrations when compared to the real textile effluent concentration.					
35821317	8	101	theme	final	1286:1290	arg1	effectiveness					1300:1312	the composite membrane final removal effectiveness	1263:1312	the composite membrane final removal effectiveness for MB 92.42%	1263:1326	After reaching adsorption equilibrium, the composite membrane final removal effectiveness for MB 92.42%, Rh.					
35821317	8	101	theme	final	1286:1290	arg1	equilibrium					1250:1260	adsorption equilibrium	1239:1260	adsorption equilibrium	1239:1260	After reaching adsorption equilibrium, the composite membrane final removal effectiveness for MB 92.42%, Rh.					
35821317	3	102	theme	various	455:461	arg1	techniques					463:472	various techniques	455:472	various techniques	455:472	The combining usage of nanomaterials and polymer material to solve these issues using various techniques.					
35821317	11	103	theme	industry	1555:1562	arg1	sample					1573:1578	textile industry effluent sample	1547:1578	textile industry effluent sample	1547:1578	Also, when the fabricated material was tested with textile industry effluent sample, all physio-chemical properties exhibited a considerable decrease in concentrations when compared to the real textile effluent concentration.					
35821317	5	104	theme	atomic	953:958	arg1	microscopy					966:975	atomic force microscopy	953:975	atomic force microscopy (AFM)	953:981	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	5	104	theme	atomic	953:958	arg1	AFM					978:980	AFM	978:980	AFM	978:980	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
35821317	7	105	theme	methyl	1185:1190	arg1	orange					1192:1197	methyl orange	1185:1197	methyl orange (MO)	1185:1202	B), methyl orange (MO) and Congo red (CR).					
35821317	7	105	theme	methyl	1185:1190	arg1	MO					1200:1201	MO	1200:1201	MO	1200:1201	B), methyl orange (MO) and Congo red (CR).					
35821317	4	106	theme	sulfone	606:612	arg1	polymer					626:632	poly-ether sulfone (PES) blend polymer	595:632	poly-ether sulfone (PES) blend polymer	595:632	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	4	106	theme	sulfone	606:612	arg1	cellulose-acetate					571:587	cellulose-acetate	571:587	cellulose-acetate (CA)	571:592	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	8	107	theme	MB	1318:1319	arg1	%					1326:1326	MB 92.42%	1318:1326	MB 92.42%	1318:1326	After reaching adsorption equilibrium, the composite membrane final removal effectiveness for MB 92.42%, Rh.					
35821317	11	108	theme	effluent	1698:1705	arg1	concentration					1707:1719	the real textile effluent concentration	1681:1719	the real textile effluent concentration	1681:1719	Also, when the fabricated material was tested with textile industry effluent sample, all physio-chemical properties exhibited a considerable decrease in concentrations when compared to the real textile effluent concentration.					
35821317	0	109	dep	nanocomposites	7:20	arg1	assimilated					22:32	assimilated	22:32	nanocomposites assimilated into CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater	7:115	GO-CuO nanocomposites assimilated into CA-PES polymer membrane in adsorptive removal of organic dyes from wastewater.					
35821317	6	110	theme	Dye	1053:1055	arg1	experiments					1068:1078	Dye adsorption experiments	1053:1078	Dye adsorption experiments	1053:1078	Dye adsorption experiments were performed with four typical water-soluble organic dyes methylene blue (MB), rhodamine blue (Rh.					
35821317	4	111	theme	PES	615:617	arg1	polymer					626:632	poly-ether sulfone (PES) blend polymer	595:632	poly-ether sulfone (PES) blend polymer	595:632	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	4	111	theme	PES	615:617	arg1	cellulose-acetate					571:587	cellulose-acetate	571:587	cellulose-acetate (CA)	571:592	In this research, graphene oxide-copper oxide (GO-CuO) nanomaterial have been incorporated into cellulose-acetate (CA), poly-ether sulfone (PES) blend polymer by using phase inversion process to fabricate thin film nanocomposite (TFN) membrane for removal of dye pollutant.					
35821317	5	112	dep	Fourier	821:827	arg1	transform					829:837	transform	829:837	transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis	829:1050	The physiochemical properties of prepared TFN materials were studied by Fourier transform infra-red spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), thermo gravimetric analysis (TGA), and mechanical strength analysis.					
37245749	5	0	dep	E.	1090:1091	arg1	coli					1093:1096	coli	1093:1096	coli	1093:1096	ZnPc(COOH)8:PMB@gel facilitated the complete healing of wounds infected with E. coli bacteria in about seven days, while over 10 % wounds treated with ZnPc(COOH)8 or PMB remained unhealed on the 9th day.					
37245749	6	1	dep	COOH	1222:1225	arg1	resulted					1233:1240	resulted	1233:1240	resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability	1233:1407	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	7	2	theme	combined	1491:1498	arg1	strategy					1514:1521	the combined antimicrobial strategy	1487:1521	the combined antimicrobial strategy	1487:1521	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy can be applied to other photosensitizers and antibiotics for detection and treatment of wound infections.					
37245749	7	3	theme	antibacterial	1460:1472	arg1	platform					1474:1481	the thermosensitive antibacterial platform	1440:1481	the thermosensitive antibacterial platform	1440:1481	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy can be applied to other photosensitizers and antibiotics for detection and treatment of wound infections.					
37245749	3	4	theme	E.	632:633	arg1	bacteria					640:647	E. coli bacteria	632:647	E. coli bacteria at 37 °C	632:656	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	5	5	theme	ZnPc	1164:1167	arg1	COOH					1169:1172	ZnPc(COOH)8	1164:1174	ZnPc(COOH)8	1164:1174	ZnPc(COOH)8:PMB@gel facilitated the complete healing of wounds infected with E. coli bacteria in about seven days, while over 10 % wounds treated with ZnPc(COOH)8 or PMB remained unhealed on the 9th day.					
37245749	1	6	theme	consequent	222:231	arg1	healing					238:244	consequent slow healing	222:244	consequent slow healing of infected wounds	222:263	Recently, rapid acquisition of bacterial resistance and consequent slow healing of infected wounds threaten human life and health.					
37245749	2	7	theme	@	521:521	arg1	gel					522:524	a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel	465:524	a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel	465:524	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	4	8	theme	E.	816:817	arg1	bacteria					824:831	E. coli bacteria	816:831	E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM)	816:879	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	2	9	theme	nanocomplexes	341:353	arg1	ZnPc					355:358	nanocomplexes ZnPc	341:358	nanocomplexes ZnPc(COOH)8:PMB	341:369	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	2	9	theme	nanocomplexes	341:353	arg1	COOH					360:363	COOH	360:363	COOH	360:363	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	1	10	theme	infected	249:256	arg1	wounds					258:263	infected wounds	249:263	infected wounds	249:263	Recently, rapid acquisition of bacterial resistance and consequent slow healing of infected wounds threaten human life and health.					
37245749	4	11	theme	bacteria	824:831	arg1	amount					806:811	a certain amount	796:811	a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM)	796:879	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	4	11	theme	bacteria	824:831	arg1	bacteria					824:831	E. coli bacteria	816:831	E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM)	816:879	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	4	12	theme	ZnPc	943:946	arg1	COOH					948:951	ZnPc(COOH)8	943:953	ZnPc(COOH)8	943:953	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	3	13	theme	aureus	673:678	arg1	bacteria					680:687	S. aureus bacteria	670:687	S. aureus bacteria	670:687	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	4	14	theme	combined	980:987	arg1	efficacy					1003:1010	combined antibacterial efficacy	980:1010	combined antibacterial efficacy	980:1010	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	3	15	theme	oxygen	568:573	arg1	species					575:581	reactive oxygen species	559:581	reactive oxygen species (ROS) of ZnPc(COOH)	559:601	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	3	15	theme	oxygen	568:573	arg1	COOH					597:600	COOH	597:600	COOH	597:600	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	3	15	theme	oxygen	568:573	arg1	ROS					584:586	ROS	584:586	ROS	584:586	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	6	16	theme	COOH	1274:1277	arg1	fluorescence					1281:1292	ZnPc(COOH)8 fluorescence	1269:1292	ZnPc(COOH)8 fluorescence in E. coli bacteria	1269:1312	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	3	17	theme	Gram-negative	750:762	arg1	bacteria					764:771	Gram-negative bacteria	750:771	Gram-negative bacteria	750:771	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	3	18	theme	ZnPc	592:595	arg1	species					575:581	reactive oxygen species	559:581	reactive oxygen species (ROS) of ZnPc(COOH)	559:601	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	3	18	theme	ZnPc	592:595	arg1	COOH					597:600	COOH	597:600	COOH	597:600	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	3	18	theme	ZnPc	592:595	arg1	ROS					584:586	ROS	584:586	ROS	584:586	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	6	19	theme	ZnPc	1269:1272	arg1	fluorescence					1281:1292	ZnPc(COOH)8 fluorescence	1269:1292	ZnPc(COOH)8 fluorescence in E. coli bacteria	1269:1312	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	1	20	theme	rapid	176:180	arg1	acquisition					182:192	rapid acquisition	176:192	rapid acquisition of bacterial resistance	176:216	Recently, rapid acquisition of bacterial resistance and consequent slow healing of infected wounds threaten human life and health.					
37245749	2	21	theme	platform	497:504	arg1	gel					522:524	a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel	465:524	a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel	465:524	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	0	22	theme	chemo-photodynamic	96:113	arg1	elimination					115:125	and combined chemo-photodynamic elimination	83:125	elimination	115:125	Construction of chitosan-based thermosensitive composite hydrogels for recognizing and combined chemo-photodynamic elimination of Gram-negative bacterial infections.					
37245749	1	23	theme	resistance	207:216	arg1	healing					238:244	consequent slow healing	222:244	consequent slow healing of infected wounds	222:263	Recently, rapid acquisition of bacterial resistance and consequent slow healing of infected wounds threaten human life and health.					
37245749	1	23	theme	resistance	207:216	arg1	acquisition					182:192	rapid acquisition	176:192	rapid acquisition of bacterial resistance	176:216	Recently, rapid acquisition of bacterial resistance and consequent slow healing of infected wounds threaten human life and health.					
37245749	6	24	theme	membrane	1387:1394	arg1	permeability					1396:1407	membrane permeability	1387:1407	membrane permeability	1387:1407	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	5	25	theme	complete	1049:1056	arg1	healing					1058:1064	the complete healing	1045:1064	the complete healing of wounds infected with E. coli bacteria	1045:1105	ZnPc(COOH)8:PMB@gel facilitated the complete healing of wounds infected with E. coli bacteria in about seven days, while over 10 % wounds treated with ZnPc(COOH)8 or PMB remained unhealed on the 9th day.					
37245749	6	26	theme	PMB	1380:1382	arg1	intervention					1364:1375	the intervention	1360:1375	the intervention of PMB on membrane permeability	1360:1407	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	3	27	from	37 °C	652:656	arg1	bacteria					640:647	E. coli bacteria	632:647	E. coli bacteria at 37 °C	632:656	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	0	28	theme	bacterial	144:152	arg1	infections					154:163	Gram-negative bacterial infections	130:163	Gram-negative bacterial infections	130:163	Construction of chitosan-based thermosensitive composite hydrogels for recognizing and combined chemo-photodynamic elimination of Gram-negative bacterial infections.					
37245749	7	29	theme	wound	1611:1615	arg1	infections					1617:1626	wound infections	1611:1626	wound infections	1611:1626	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy can be applied to other photosensitizers and antibiotics for detection and treatment of wound infections.					
37245749	5	30	theme	E.	1090:1091	arg1	bacteria					1098:1105	E. coli bacteria	1090:1105	E. coli bacteria	1090:1105	ZnPc(COOH)8:PMB@gel facilitated the complete healing of wounds infected with E. coli bacteria in about seven days, while over 10 % wounds treated with ZnPc(COOH)8 or PMB remained unhealed on the 9th day.					
37245749	0	31	theme	chitosan-based	16:29	arg1	hydrogels					57:65	chitosan-based thermosensitive composite hydrogels	16:65	chitosan-based thermosensitive composite hydrogels	16:65	Construction of chitosan-based thermosensitive composite hydrogels for recognizing and combined chemo-photodynamic elimination of Gram-negative bacterial infections.					
37245749	2	32	theme	thermosensitive	467:481	arg1	gel					522:524	a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel	465:524	a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel	465:524	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	2	33	theme	antibiotic	415:424	arg1	PMB					439:441	PMB	439:441	PMB	439:441	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	2	33	theme	antibiotic	415:424	arg1	B					436:436	antibiotic polymyxin B	415:436	antibiotic polymyxin B (PMB)	415:442	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	0	34	theme	composite	47:55	arg1	hydrogels					57:65	chitosan-based thermosensitive composite hydrogels	16:65	chitosan-based thermosensitive composite hydrogels	16:65	Construction of chitosan-based thermosensitive composite hydrogels for recognizing and combined chemo-photodynamic elimination of Gram-negative bacterial infections.					
37245749	3	35	dep	E.	632:633	arg1	coli					635:638	coli	635:638	coli	635:638	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	6	36	theme	ZnPc	1217:1220	arg1	COOH					1222:1225	ZnPc(COOH)8	1217:1227	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.	1217:1408	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	1	37	theme	human	274:278	arg1	life					280:283	human life	274:283	human life	274:283	Recently, rapid acquisition of bacterial resistance and consequent slow healing of infected wounds threaten human life and health.					
37245749	7	38	theme	antimicrobial	1500:1512	arg1	strategy					1514:1521	the combined antimicrobial strategy	1487:1521	the combined antimicrobial strategy	1487:1521	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy can be applied to other photosensitizers and antibiotics for detection and treatment of wound infections.					
37245749	3	39	theme	PMB	604:606	arg1	gel					608:610	fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel	542:610	gel	608:610	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	6	40	from	bacteria	1305:1312	arg1	increase					1257:1264	a threefold increase	1245:1264	a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria	1245:1312	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	5	41	theme	10 	1139:1141	arg1	%					1142:1142	%	1142:1142	%	1142:1142	ZnPc(COOH)8:PMB@gel facilitated the complete healing of wounds infected with E. coli bacteria in about seven days, while over 10 % wounds treated with ZnPc(COOH)8 or PMB remained unhealed on the 9th day.					
37245749	5	42	theme	ZnPc	1013:1016	arg1	gel					1029:1031	ZnPc(COOH)8:PMB@gel	1013:1031	ZnPc(COOH)8:PMB@gel	1013:1031	ZnPc(COOH)8:PMB@gel facilitated the complete healing of wounds infected with E. coli bacteria in about seven days, while over 10 % wounds treated with ZnPc(COOH)8 or PMB remained unhealed on the 9th day.					
37245749	7	43	theme	platform	1474:1481	arg1	principle					1427:1435	The construction principle	1410:1435	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy	1410:1521	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy can be applied to other photosensitizers and antibiotics for detection and treatment of wound infections.					
37245749	5	44	theme	COOH	1018:1021	arg1	gel					1029:1031	ZnPc(COOH)8:PMB@gel	1013:1031	ZnPc(COOH)8:PMB@gel	1013:1031	ZnPc(COOH)8:PMB@gel facilitated the complete healing of wounds infected with E. coli bacteria in about seven days, while over 10 % wounds treated with ZnPc(COOH)8 or PMB remained unhealed on the 9th day.					
37245749	4	45	theme	survival	778:785	arg1	rate					787:790	The survival rate	774:790	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM)	774:879	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	7	46	theme	thermosensitive	1444:1458	arg1	platform					1474:1481	the thermosensitive antibacterial platform	1440:1481	the thermosensitive antibacterial platform	1440:1481	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy can be applied to other photosensitizers and antibiotics for detection and treatment of wound infections.					
37245749	2	47	theme	chitosan-based	312:325	arg1	hydrogels					327:335	chitosan-based hydrogels	312:335	chitosan-based hydrogels	312:335	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	3	48	theme	fluorescence	542:553	arg1	gel					608:610	fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel	542:610	gel	608:610	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	1	49	theme	slow	233:236	arg1	healing					238:244	consequent slow healing	222:244	consequent slow healing of infected wounds	222:263	Recently, rapid acquisition of bacterial resistance and consequent slow healing of infected wounds threaten human life and health.					
37245749	7	50	theme	construction	1414:1425	arg1	principle					1427:1435	The construction principle	1410:1435	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy	1410:1521	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy can be applied to other photosensitizers and antibiotics for detection and treatment of wound infections.					
37245749	3	51	theme	species	575:581	arg1	gel					608:610	fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel	542:610	gel	608:610	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	1	52	theme	wounds	258:263	arg1	healing					238:244	consequent slow healing	222:244	consequent slow healing of infected wounds	222:263	Recently, rapid acquisition of bacterial resistance and consequent slow healing of infected wounds threaten human life and health.					
37245749	1	52	theme	wounds	258:263	arg1	acquisition					182:192	rapid acquisition	176:192	rapid acquisition of bacterial resistance	176:216	Recently, rapid acquisition of bacterial resistance and consequent slow healing of infected wounds threaten human life and health.					
37245749	4	53	theme	antibacterial	989:1001	arg1	efficacy					1003:1010	combined antibacterial efficacy	980:1010	combined antibacterial efficacy	980:1010	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	3	54	theme	reactive	559:566	arg1	species					575:581	reactive oxygen species	559:581	reactive oxygen species (ROS) of ZnPc(COOH)	559:601	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	3	54	theme	reactive	559:566	arg1	COOH					597:600	COOH	597:600	COOH	597:600	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	3	54	theme	reactive	559:566	arg1	ROS					584:586	ROS	584:586	ROS	584:586	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	3	55	theme	S.	670:671	arg1	bacteria					680:687	S. aureus bacteria	670:687	S. aureus bacteria	670:687	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	4	56	theme	certain	798:804	arg1	amount					806:811	a certain amount	796:811	a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM)	796:879	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	4	56	theme	certain	798:804	arg1	bacteria					824:831	E. coli bacteria	816:831	E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM)	816:879	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	6	57	theme	ZnPc	1344:1347	arg1	uptake					1334:1339	enhanced uptake	1325:1339	enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability	1325:1407	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	5	58	theme	wounds	1069:1074	arg1	healing					1058:1064	the complete healing	1045:1064	the complete healing of wounds infected with E. coli bacteria	1045:1105	ZnPc(COOH)8:PMB@gel facilitated the complete healing of wounds infected with E. coli bacteria in about seven days, while over 10 % wounds treated with ZnPc(COOH)8 or PMB remained unhealed on the 9th day.					
37245749	6	59	from	increase	1257:1264	arg1	bacteria					1305:1312	E. coli bacteria	1297:1312	E. coli bacteria	1297:1312	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	2	60	theme	ZnPc	506:509	arg1	gel					522:524	a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel	465:524	a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel	465:524	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	6	61	theme	threefold	1247:1255	arg1	increase					1257:1264	a threefold increase	1245:1264	a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria	1245:1312	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	1	62	theme	bacterial	197:205	arg1	resistance					207:216	bacterial resistance	197:216	bacterial resistance	197:216	Recently, rapid acquisition of bacterial resistance and consequent slow healing of infected wounds threaten human life and health.					
37245749	2	63	theme	antibacterial	483:495	arg1	gel					522:524	a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel	465:524	a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel	465:524	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	0	64	theme	Gram-negative	130:142	arg1	infections					154:163	Gram-negative bacterial infections	130:163	Gram-negative bacterial infections	130:163	Construction of chitosan-based thermosensitive composite hydrogels for recognizing and combined chemo-photodynamic elimination of Gram-negative bacterial infections.					
37245749	6	65	theme	enhanced	1325:1332	arg1	uptake					1334:1339	enhanced uptake	1325:1339	enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability	1325:1407	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	6	66	from	intervention	1364:1375	arg1	permeability					1396:1407	membrane permeability	1387:1407	membrane permeability	1387:1407	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	0	67	theme	infections	154:163	arg1	elimination					115:125	and combined chemo-photodynamic elimination	83:125	elimination	115:125	Construction of chitosan-based thermosensitive composite hydrogels for recognizing and combined chemo-photodynamic elimination of Gram-negative bacterial infections.					
37245749	7	68	theme	infections	1617:1626	arg1	detection					1584:1592	detection	1584:1592	detection	1584:1592	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy can be applied to other photosensitizers and antibiotics for detection and treatment of wound infections.					
37245749	7	68	theme	infections	1617:1626	arg1	treatment					1598:1606	treatment	1598:1606	treatment	1598:1606	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy can be applied to other photosensitizers and antibiotics for detection and treatment of wound infections.					
37245749	6	69	from	fluorescence	1281:1292	arg1	bacteria					1305:1312	E. coli bacteria	1297:1312	E. coli bacteria	1297:1312	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	4	70	theme	ZnPc	846:849	arg1	COOH					851:854	ZnPc(COOH)8:PMB	846:860	ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM)	846:879	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	4	70	theme	ZnPc	846:849	arg1	2 μM					875:878	ZnPc(COOH)8 2 μM	863:878	ZnPc(COOH)8 2 μM	863:878	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	4	71	dep	E.	816:817	arg1	coli					819:822	coli	819:822	coli	819:822	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	6	72	theme	E.	1297:1298	arg1	bacteria					1305:1312	E. coli bacteria	1297:1312	E. coli bacteria	1297:1312	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	0	73	dep	elimination	115:125	arg1	combined					87:94	combined	87:94	combined	87:94	Construction of chitosan-based thermosensitive composite hydrogels for recognizing and combined chemo-photodynamic elimination of Gram-negative bacterial infections.					
37245749	6	74	dep	E.	1297:1298	arg1	coli					1300:1303	coli	1300:1303	coli	1300:1303	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	4	75	theme	COOH	868:871	arg1	COOH					851:854	ZnPc(COOH)8:PMB	846:860	ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM)	846:879	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	4	75	theme	COOH	868:871	arg1	2 μM					875:878	ZnPc(COOH)8 2 μM	863:878	ZnPc(COOH)8 2 μM	863:878	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	5	76	theme	9th	1208:1210	arg1	day					1212:1214	the 9th day	1204:1214	the 9th day	1204:1214	ZnPc(COOH)8:PMB@gel facilitated the complete healing of wounds infected with E. coli bacteria in about seven days, while over 10 % wounds treated with ZnPc(COOH)8 or PMB remained unhealed on the 9th day.					
37245749	0	77	theme	thermosensitive	31:45	arg1	hydrogels					57:65	chitosan-based thermosensitive composite hydrogels	16:65	chitosan-based thermosensitive composite hydrogels	16:65	Construction of chitosan-based thermosensitive composite hydrogels for recognizing and combined chemo-photodynamic elimination of Gram-negative bacterial infections.					
37245749	6	78	theme	fluorescence	1281:1292	arg1	increase					1257:1264	a threefold increase	1245:1264	a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria	1245:1312	ZnPc(COOH)8:PMB resulted in a threefold increase of ZnPc(COOH)8 fluorescence in E. coli bacteria suggesting enhanced uptake of ZnPc(COOH)8 for the intervention of PMB on membrane permeability.					
37245749	2	79	theme	polymyxin	426:434	arg1	PMB					439:441	PMB	439:441	PMB	439:441	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	2	79	theme	polymyxin	426:434	arg1	B					436:436	antibiotic polymyxin B	415:436	antibiotic polymyxin B (PMB)	415:442	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	0	80	theme	hydrogels	57:65	arg1	Construction					0:11	Construction	0:11	Construction of chitosan-based thermosensitive composite hydrogels for recognizing and combined chemo-photodynamic elimination of Gram-negative bacterial infections.	0:164	Construction of chitosan-based thermosensitive composite hydrogels for recognizing and combined chemo-photodynamic elimination of Gram-negative bacterial infections.					
37245749	5	81	theme	%	1142:1142	arg1	wounds					1144:1149	10 % wounds	1139:1149	10 % wounds treated with ZnPc(COOH)8 or PMB	1139:1181	ZnPc(COOH)8:PMB@gel facilitated the complete healing of wounds infected with E. coli bacteria in about seven days, while over 10 % wounds treated with ZnPc(COOH)8 or PMB remained unhealed on the 9th day.					
37245749	2	82	theme	COOH	511:514	arg1	gel					522:524	a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel	465:524	a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel	465:524	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	4	83	theme	ZnPc	863:866	arg1	COOH					851:854	ZnPc(COOH)8:PMB	846:860	ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM)	846:879	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	4	83	theme	ZnPc	863:866	arg1	2 μM					875:878	ZnPc(COOH)8 2 μM	863:878	ZnPc(COOH)8 2 μM	863:878	The survival rate for a certain amount of E. coli bacteria treated with ZnPc(COOH)8:PMB (ZnPc(COOH)8 2 μM) was decreased by approximately fivefold than that with either ZnPc(COOH)8 or PMB alone, indicating combined antibacterial efficacy.					
37245749	3	84	theme	@	607:607	arg1	gel					608:610	fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel	542:610	gel	608:610	Interestingly, fluorescence and reactive oxygen species (ROS) of ZnPc(COOH)8:PMB@gel can be triggered by E. coli bacteria at 37 °C, but not by S. aureus bacteria, which gave the potential to simultaneously detect and treat Gram-negative bacteria.					
37245749	7	85	theme	other	1541:1545	arg1	photosensitizers					1547:1562	other photosensitizers	1541:1562	other photosensitizers	1541:1562	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy can be applied to other photosensitizers and antibiotics for detection and treatment of wound infections.					
37245749	7	86	theme	strategy	1514:1521	arg1	principle					1427:1435	The construction principle	1410:1435	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy	1410:1521	The construction principle of the thermosensitive antibacterial platform and the combined antimicrobial strategy can be applied to other photosensitizers and antibiotics for detection and treatment of wound infections.					
37245749	2	87	theme	photosensitizer	383:397	arg1	COOH					404:407	COOH	404:407	COOH	404:407	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	2	87	theme	photosensitizer	383:397	arg1	ZnPc					399:402	photosensitizer ZnPc(COOH)8	383:409	photosensitizer ZnPc(COOH)8	383:409	In this study, chitosan-based hydrogels and nanocomplexes ZnPc(COOH)8:PMB composed of photosensitizer ZnPc(COOH)8 and antibiotic polymyxin B (PMB) were integrated into a thermosensitive antibacterial platform ZnPc(COOH)8:PMB@gel.					
37245749	5	88	theme	@	1028:1028	arg1	gel					1029:1031	ZnPc(COOH)8:PMB@gel	1013:1031	ZnPc(COOH)8:PMB@gel	1013:1031	ZnPc(COOH)8:PMB@gel facilitated the complete healing of wounds infected with E. coli bacteria in about seven days, while over 10 % wounds treated with ZnPc(COOH)8 or PMB remained unhealed on the 9th day.					
36940825	8	0	theme	high	1590:1593	arg1	efficiency					1603:1612	the high removal efficiency	1586:1612	the high removal efficiency	1586:1612	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	7	1	theme	highest	1248:1254	arg1	capacity					1267:1274	the highest adsorption capacity	1244:1274	the highest adsorption capacity	1244:1274	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	3	2	theme	lightweight	468:478	arg1	It					435:436	It	435:436	It	435:436	It is also the most inexpensive, lightweight, and globally friendly material tested.					
36940825	3	2	theme	lightweight	468:478	arg1	material					503:510	the most inexpensive, lightweight, and globally friendly material	446:510	the most inexpensive, lightweight, and globally friendly material tested	446:517	It is also the most inexpensive, lightweight, and globally friendly material tested.					
36940825	8	3	theme	positive	1491:1498	arg1	charge					1500:1505	the positive charge	1487:1505	the positive charge chitosan on the surface of PUF	1487:1536	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	5	4	theme	surface	785:791	arg1	presence					763:770	The presence	759:770	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF	759:839	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	9	5	theme	fixed-bed	1651:1659	arg1	studies					1668:1674	fixed-bed column studies	1651:1674	fixed-bed column studies	1651:1674	This was done through a sequence of fixed-bed column studies, which took place in the continuous flowing of wastewater.					
36940825	6	6	dep	the	1002:1004	arg1	help					1006:1009	help	1006:1009	help	1006:1009	With the help of the ion exchange mechanism and electrostatic contact, negatively charged MXene hexavalent ions were being adsorbed on the surface.					
36940825	6	7	theme	MXene	1087:1091	arg1	ions					1104:1107	negatively charged MXene hexavalent ions	1068:1107	negatively charged MXene hexavalent ions	1068:1107	With the help of the ion exchange mechanism and electrostatic contact, negatively charged MXene hexavalent ions were being adsorbed on the surface.					
36940825	8	8	theme	chitosan	1507:1514	arg1	charge					1500:1505	the positive charge	1487:1505	the positive charge chitosan on the surface of PUF	1487:1536	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	7	9	theme	different	1214:1222	arg1	layers					1224:1229	three different layers	1208:1229	three different layers	1208:1229	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	9	10	theme	studies	1668:1674	arg1	sequence					1639:1646	a sequence	1637:1646	a sequence	1637:1646	This was done through a sequence of fixed-bed column studies, which took place in the continuous flowing of wastewater.					
36940825	5	11	theme	@	937:937	arg1	PUF					938:940	MX3@CS3@PUF	930:940	MX3@CS3@PUF	930:940	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	3	12	theme	friendly	494:501	arg1	It					435:436	It	435:436	It	435:436	It is also the most inexpensive, lightweight, and globally friendly material tested.					
36940825	3	12	theme	friendly	494:501	arg1	material					503:510	the most inexpensive, lightweight, and globally friendly material	446:510	the most inexpensive, lightweight, and globally friendly material tested	446:517	It is also the most inexpensive, lightweight, and globally friendly material tested.					
36940825	1	13	theme	aqueous	179:185	arg1	atmosphere					187:196	the aqueous atmosphere	175:196	the aqueous atmosphere	175:196	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	6	14	theme	charged	1079:1085	arg1	ions					1104:1107	negatively charged MXene hexavalent ions	1068:1107	negatively charged MXene hexavalent ions	1068:1107	With the help of the ion exchange mechanism and electrostatic contact, negatively charged MXene hexavalent ions were being adsorbed on the surface.					
36940825	7	15	dep	elimination	1352:1362	arg1	%					1350:1350	%	1350:1350	%	1350:1350	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	0	16	theme	surface-engineered	29:46	arg1	composite					54:62	high-capacity surface-engineered MXene composite	15:62	high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater	15:121	Development of high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater.					
36940825	1	17	from	atmosphere	187:196	arg1	fear					223:226	a major environmental fear	201:226	a major environmental fear that cannot be overlooked	201:252	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	1	17	from	atmosphere	187:196	arg1	quantity					140:147	The substantial quantity	124:147	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere	124:196	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	7	18	theme	metal	1383:1387	arg1	concentration					1393:1405	the metal ion concentration	1379:1405	the metal ion concentration	1379:1405	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	7	18	theme	metal	1383:1387	arg1	20 ppm					1411:1416	20 ppm	1411:1416	20 ppm	1411:1416	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	5	19	theme	MX3	829:831	arg1	PUF					837:839	the Mxene- MX3@CS3@PUF	818:839	the Mxene- MX3@CS3@PUF	818:839	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	6	20	theme	electrostatic	1045:1057	arg1	contact					1059:1065	electrostatic contact	1045:1065	electrostatic contact	1045:1065	With the help of the ion exchange mechanism and electrostatic contact, negatively charged MXene hexavalent ions were being adsorbed on the surface.					
36940825	4	21	theme	electron	669:676	arg1	microscope					678:687	scanning electron microscope	660:687	scanning electron microscope	660:687	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	4	21	theme	electron	669:676	arg1	SEM					655:657	SEM	655:657	SEM (scanning electron microscope)	655:688	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	8	22	theme	PUF	1534:1536	arg1	surface					1523:1529	the surface	1519:1529	the surface of PUF	1519:1536	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	5	23	theme	@	933:933	arg1	PUF					938:940	MX3@CS3@PUF	930:940	MX3@CS3@PUF	930:940	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	6	24	theme	ion	1018:1020	arg1	mechanism					1031:1039	the ion exchange mechanism	1014:1039	the ion exchange mechanism	1014:1039	With the help of the ion exchange mechanism and electrostatic contact, negatively charged MXene hexavalent ions were being adsorbed on the surface.					
36940825	5	25	theme	CS3	833:835	arg1	PUF					837:839	the Mxene- MX3@CS3@PUF	818:839	the Mxene- MX3@CS3@PUF	818:839	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	0	26	theme	metal	74:78	arg1	removal					88:94	heavy metal Cr (VI) removal	68:94	heavy metal Cr (VI) removal from industrial wastewater	68:121	Development of high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater.					
36940825	5	27	theme	creation	806:813	arg1	presence					763:770	The presence	759:770	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF	759:839	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	9	28	theme	wastewater	1723:1732	arg1	flowing					1712:1718	the continuous flowing	1697:1718	the continuous flowing of wastewater	1697:1732	This was done through a sequence of fixed-bed column studies, which took place in the continuous flowing of wastewater.					
36940825	1	29	from	Cr	152:153	arg1	atmosphere					187:196	the aqueous atmosphere	175:196	the aqueous atmosphere	175:196	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	4	30	theme	X-ray	696:700	arg1	XPS					691:693	XPS	691:693	XPS (X-ray photoelectron spectroscopy)	691:728	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	4	30	theme	X-ray	696:700	arg1	spectroscopy					716:727	X-ray photoelectron spectroscopy	696:727	X-ray photoelectron spectroscopy	696:727	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	1	31	from	quantity	140:147	arg1	atmosphere					187:196	the aqueous atmosphere	175:196	the aqueous atmosphere	175:196	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	5	32	theme	surface	857:863	arg1	area					865:868	its surface area	853:868	its surface area	853:868	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	1	33	theme	substantial	128:138	arg1	fear					223:226	a major environmental fear	201:226	a major environmental fear that cannot be overlooked	201:252	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	1	33	theme	substantial	128:138	arg1	quantity					140:147	The substantial quantity	124:147	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere	124:196	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	2	34	theme	heavy	374:378	arg1	ions					386:389	heavy metal ions	374:389	heavy metal ions (Cr (VI))	374:399	For the first time, MXene and chitosan-coated polyurethane foam have been employed for wastewater treatment, including heavy metal ions (Cr (VI)) through a fixed-bed column study.					
36940825	4	35	dep	transform	634:642	arg1	infrared					644:651	infrared	644:651	transform infrared	634:651	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	7	36	from	foam	1188:1191	arg1	form					1200:1203	the form	1196:1203	the form of three different layers, which shows the highest adsorption capacity	1196:1274	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	1	37	dep	Cr	152:153	arg1	contaminants					159:170	contaminants	159:170	Cr(VI) contaminants in the aqueous atmosphere	152:196	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	1	37	dep	Cr	152:153	arg1	VI					155:156	VI	155:156	VI	155:156	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	8	38	theme	@	1561:1561	arg1	PUF					1562:1564	MX@PUF	1559:1564	MX@PUF	1559:1564	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	0	39	theme	high-capacity	15:27	arg1	composite					54:62	high-capacity surface-engineered MXene composite	15:62	high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater	15:121	Development of high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater.					
36940825	7	40	dep	%	1292:1292	arg1	∼70					1289:1291	∼70	1289:1291	∼70	1289:1291	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	2	41	theme	column	421:426	arg1	study					428:432	a fixed-bed column study	409:432	a fixed-bed column study	409:432	For the first time, MXene and chitosan-coated polyurethane foam have been employed for wastewater treatment, including heavy metal ions (Cr (VI)) through a fixed-bed column study.					
36940825	2	42	dep	ions	386:389	arg1	Cr					392:393	Cr	392:393	Cr	392:393	For the first time, MXene and chitosan-coated polyurethane foam have been employed for wastewater treatment, including heavy metal ions (Cr (VI)) through a fixed-bed column study.					
36940825	0	43	theme	MXene	48:52	arg1	composite					54:62	high-capacity surface-engineered MXene composite	15:62	high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater	15:121	Development of high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater.					
36940825	2	44	dep	Cr	392:393	arg1	VI					396:397	VI	396:397	VI	396:397	For the first time, MXene and chitosan-coated polyurethane foam have been employed for wastewater treatment, including heavy metal ions (Cr (VI)) through a fixed-bed column study.					
36940825	5	45	attach	presence	763:770	arg2	surface					785:791	the rough surface	775:791	the rough surface	775:791	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	5	45	attach	presence	763:770	arg1	PUF					837:839	the Mxene- MX3@CS3@PUF	818:839	the Mxene- MX3@CS3@PUF	818:839	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	5	45	attach	presence	763:770	arg2	creation					806:813	the pore creation	797:813	the pore creation	797:813	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	6	46	theme	exchange	1022:1029	arg1	mechanism					1031:1039	the ion exchange mechanism	1014:1039	the ion exchange mechanism	1014:1039	With the help of the ion exchange mechanism and electrostatic contact, negatively charged MXene hexavalent ions were being adsorbed on the surface.					
36940825	2	47	theme	polyurethane	301:312	arg1	foam					314:317	chitosan-coated polyurethane foam	285:317	chitosan-coated polyurethane foam	285:317	For the first time, MXene and chitosan-coated polyurethane foam have been employed for wastewater treatment, including heavy metal ions (Cr (VI)) through a fixed-bed column study.					
36940825	7	48	dep	removed	1306:1312	arg1	VI					1298:1299	VI	1298:1299	VI	1298:1299	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	4	49	theme	chitosan-coated	534:548	arg1	materials					575:583	The Mxene and chitosan-coated polyurethane foam hybrid materials	520:583	The Mxene and chitosan-coated polyurethane foam hybrid materials	520:583	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	2	50	theme	wastewater	342:351	arg1	treatment					353:361	wastewater treatment	342:361	wastewater treatment	342:361	For the first time, MXene and chitosan-coated polyurethane foam have been employed for wastewater treatment, including heavy metal ions (Cr (VI)) through a fixed-bed column study.					
36940825	8	51	theme	electrostatic	1423:1435	arg1	accountable					1570:1580	accountable	1570:1580	accountable	1570:1580	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	8	51	theme	electrostatic	1423:1435	arg1	interaction					1437:1447	The electrostatic interaction	1419:1447	The electrostatic interaction	1419:1447	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	8	51	theme	electrostatic	1423:1435	arg1	absent					1549:1554	absent	1549:1554	absent	1549:1554	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	1	52	theme	environmental	209:221	arg1	fear					223:226	a major environmental fear	201:226	a major environmental fear that cannot be overlooked	201:252	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	1	52	theme	environmental	209:221	arg1	quantity					140:147	The substantial quantity	124:147	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere	124:196	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	4	53	theme	foam	563:566	arg1	materials					575:583	The Mxene and chitosan-coated polyurethane foam hybrid materials	520:583	The Mxene and chitosan-coated polyurethane foam hybrid materials	520:583	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	8	54	theme	removal	1595:1601	arg1	efficiency					1603:1612	the high removal efficiency	1586:1612	the high removal efficiency	1586:1612	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	2	55	theme	first	263:267	arg1	time					269:272	the first time	259:272	the first time	259:272	For the first time, MXene and chitosan-coated polyurethane foam have been employed for wastewater treatment, including heavy metal ions (Cr (VI)) through a fixed-bed column study.					
36940825	8	56	theme	negative	1461:1468	arg1	charge					1470:1475	the negative charge MXene and the positive charge chitosan on the surface of PUF	1457:1536	charge	1470:1475	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	7	57	theme	adsorption	1256:1265	arg1	capacity					1267:1274	the highest adsorption capacity	1244:1274	the highest adsorption capacity	1244:1274	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	4	58	theme	Mxene	524:528	arg1	materials					575:583	The Mxene and chitosan-coated polyurethane foam hybrid materials	520:583	The Mxene and chitosan-coated polyurethane foam hybrid materials	520:583	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	5	59	theme	rough	779:783	arg1	surface					785:791	the rough surface	775:791	the rough surface	775:791	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	6	60	theme	hexavalent	1093:1102	arg1	ions					1104:1107	negatively charged MXene hexavalent ions	1068:1107	negatively charged MXene hexavalent ions	1068:1107	With the help of the ion exchange mechanism and electrostatic contact, negatively charged MXene hexavalent ions were being adsorbed on the surface.					
36940825	9	61	theme	column	1661:1666	arg1	studies					1668:1674	fixed-bed column studies	1651:1674	fixed-bed column studies	1651:1674	This was done through a sequence of fixed-bed column studies, which took place in the continuous flowing of wastewater.					
36940825	5	62	from	assembly	918:925	arg1	solution					987:994	the aqueous solution	975:994	the aqueous solution	975:994	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	7	63	theme	ion	1389:1391	arg1	concentration					1393:1405	the metal ion concentration	1379:1405	the metal ion concentration	1379:1405	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	7	63	theme	ion	1389:1391	arg1	20 ppm					1411:1416	20 ppm	1411:1416	20 ppm	1411:1416	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	7	64	theme	layers	1224:1229	arg1	form					1200:1203	the form	1196:1203	the form of three different layers, which shows the highest adsorption capacity	1196:1274	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	8	65	from	absent	1549:1554	arg1	PUF					1562:1564	MX@PUF	1559:1564	MX@PUF	1559:1564	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	5	66	theme	PUF	938:940	arg1	Cr					950:951	the Cr(VI) contaminations in the aqueous solution	946:994	the Cr(VI) contaminations in the aqueous solution	946:994	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	5	66	theme	PUF	938:940	arg1	assembly					918:925	the surface-active assembly	899:925	the surface-active assembly of MX3@CS3@PUF	899:940	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	8	67	dep	charge	1470:1475	arg1	MXene					1477:1481	MXene	1477:1481	MXene	1477:1481	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	5	68	theme	Mxene-	822:827	arg1	PUF					837:839	the Mxene- MX3@CS3@PUF	818:839	the Mxene- MX3@CS3@PUF	818:839	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	8	69	from	PUF	1562:1564	arg1	accountable					1570:1580	accountable	1570:1580	accountable	1570:1580	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	8	69	from	PUF	1562:1564	arg1	interaction					1437:1447	The electrostatic interaction	1419:1447	The electrostatic interaction	1419:1447	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	8	69	from	PUF	1562:1564	arg1	absent					1549:1554	absent	1549:1554	absent	1549:1554	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	5	70	theme	MX3	930:932	arg1	PUF					938:940	MX3@CS3@PUF	930:940	MX3@CS3@PUF	930:940	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	5	71	from	presence	763:770	arg1	PUF					837:839	the Mxene- MX3@CS3@PUF	818:839	the Mxene- MX3@CS3@PUF	818:839	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	5	72	theme	@	832:832	arg1	PUF					837:839	the Mxene- MX3@CS3@PUF	818:839	the Mxene- MX3@CS3@PUF	818:839	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	9	73	theme	continuous	1701:1710	arg1	flowing					1712:1718	the continuous flowing	1697:1718	the continuous flowing of wastewater	1697:1732	This was done through a sequence of fixed-bed column studies, which took place in the continuous flowing of wastewater.					
36940825	4	74	theme	scanning	660:667	arg1	microscope					678:687	scanning electron microscope	660:687	scanning electron microscope	660:687	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	4	74	theme	scanning	660:667	arg1	SEM					655:657	SEM	655:657	SEM (scanning electron microscope)	655:688	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	0	75	theme	Cr	80:81	arg1	removal					88:94	heavy metal Cr (VI) removal	68:94	heavy metal Cr (VI) removal from industrial wastewater	68:121	Development of high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater.					
36940825	5	76	theme	CS3	934:936	arg1	PUF					938:940	MX3@CS3@PUF	930:940	MX3@CS3@PUF	930:940	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	5	77	theme	pore	801:804	arg1	creation					806:813	the pore creation	797:813	the pore creation	797:813	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	7	78	theme	PUF	1184:1186	arg1	foam					1188:1191	PUF foam	1184:1191	PUF foam	1184:1191	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	5	79	dep	rise	848:851	arg1	useful					880:885	useful	880:885	useful	880:885	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	1	80	theme	Cr	152:153	arg1	fear					223:226	a major environmental fear	201:226	a major environmental fear that cannot be overlooked	201:252	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	1	80	theme	Cr	152:153	arg1	quantity					140:147	The substantial quantity	124:147	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere	124:196	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	5	81	theme	aqueous	979:985	arg1	solution					987:994	the aqueous solution	975:994	the aqueous solution	975:994	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	8	82	theme	MX	1559:1560	arg1	PUF					1562:1564	MX@PUF	1559:1564	MX@PUF	1559:1564	The electrostatic interaction between the negative charge MXene and the positive charge chitosan on the surface of PUF, which was absent in MX@PUF, is accountable for the high removal efficiency.					
36940825	0	83	theme	industrial	101:110	arg1	wastewater					112:121	industrial wastewater	101:121	industrial wastewater	101:121	Development of high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater.					
36940825	0	84	dep	removal	88:94	arg1	VI					84:85	VI	84:85	VI	84:85	Development of high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater.					
36940825	4	85	theme	photoelectron	702:714	arg1	XPS					691:693	XPS	691:693	XPS (X-ray photoelectron spectroscopy)	691:728	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	4	85	theme	photoelectron	702:714	arg1	spectroscopy					716:727	X-ray photoelectron spectroscopy	696:727	X-ray photoelectron spectroscopy	696:727	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	5	86	theme	@	836:836	arg1	PUF					837:839	the Mxene- MX3@CS3@PUF	818:839	the Mxene- MX3@CS3@PUF	818:839	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	7	87	dep	%	1350:1350	arg1	60					1348:1349	60	1348:1349	60	1348:1349	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	5	88	from	Cr	950:951	arg1	solution					987:994	the aqueous solution	975:994	the aqueous solution	975:994	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	7	89	dep	∼70	1289:1291	arg1	to					1286:1287	to	1286:1287	to	1286:1287	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	0	90	theme	composite	54:62	arg1	Development					0:10	Development	0:10	Development of high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater.	0:122	Development of high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater.					
36940825	7	91	dep	Cr	1294:1295	arg1	%					1292:1292	%	1292:1292	%	1292:1292	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	7	92	theme	first	1321:1325	arg1	10 min					1327:1332	the first 10 min	1317:1332	the first 10 min	1317:1332	MXene and chitosan have been coated on PUF foam in the form of three different layers, which shows the highest adsorption capacity, where up to ∼70% Cr (VI) was removed in the first 10 min and more than 60% elimination after 3 h when the metal ion concentration was 20 ppm.					
36940825	2	93	theme	fixed-bed	411:419	arg1	study					428:432	a fixed-bed column study	409:432	a fixed-bed column study	409:432	For the first time, MXene and chitosan-coated polyurethane foam have been employed for wastewater treatment, including heavy metal ions (Cr (VI)) through a fixed-bed column study.					
36940825	4	94	theme	X-ray	739:743	arg1	XRD					734:736	XRD	734:736	XRD (X-ray diffraction)	734:756	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	4	94	theme	X-ray	739:743	arg1	diffraction					745:755	X-ray diffraction	739:755	X-ray diffraction	739:755	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	2	95	theme	metal	380:384	arg1	ions					386:389	heavy metal ions	374:389	heavy metal ions (Cr (VI))	374:399	For the first time, MXene and chitosan-coated polyurethane foam have been employed for wastewater treatment, including heavy metal ions (Cr (VI)) through a fixed-bed column study.					
36940825	0	96	theme	heavy	68:72	arg1	removal					88:94	heavy metal Cr (VI) removal	68:94	heavy metal Cr (VI) removal from industrial wastewater	68:121	Development of high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater.					
36940825	5	97	theme	surface-active	903:916	arg1	assembly					918:925	the surface-active assembly	899:925	the surface-active assembly of MX3@CS3@PUF	899:940	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	5	98	dep	Cr	950:951	arg1	VI					953:954	VI	953:954	VI	953:954	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	5	98	dep	Cr	950:951	arg1	contaminations					957:970	contaminations	957:970	the Cr(VI) contaminations in the aqueous solution	946:994	The presence of the rough surface and the pore creation in the Mxene- MX3@CS3@PUF should rise its surface area, which is useful to interact the surface-active assembly of MX3@CS3@PUF and the Cr(VI) contaminations in the aqueous solution.					
36940825	4	99	theme	polyurethane	550:561	arg1	materials					575:583	The Mxene and chitosan-coated polyurethane foam hybrid materials	520:583	The Mxene and chitosan-coated polyurethane foam hybrid materials	520:583	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	2	100	theme	chitosan-coated	285:299	arg1	foam					314:317	chitosan-coated polyurethane foam	285:317	chitosan-coated polyurethane foam	285:317	For the first time, MXene and chitosan-coated polyurethane foam have been employed for wastewater treatment, including heavy metal ions (Cr (VI)) through a fixed-bed column study.					
36940825	1	101	theme	major	203:207	arg1	fear					223:226	a major environmental fear	201:226	a major environmental fear that cannot be overlooked	201:252	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	1	101	theme	major	203:207	arg1	quantity					140:147	The substantial quantity	124:147	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere	124:196	The substantial quantity of Cr(VI) contaminants in the aqueous atmosphere is a major environmental fear that cannot be overlooked.					
36940825	4	102	dep	FTIR	620:623	arg1	transform					634:642	transform	634:642	transform infrared	634:651	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	4	103	theme	hybrid	568:573	arg1	materials					575:583	The Mxene and chitosan-coated polyurethane foam hybrid materials	520:583	The Mxene and chitosan-coated polyurethane foam hybrid materials	520:583	The Mxene and chitosan-coated polyurethane foam hybrid materials were thoroughly investigated using FTIR (Fourier transform infrared), SEM (scanning electron microscope), XPS (X-ray photoelectron spectroscopy) and XRD (X-ray diffraction).					
36940825	0	104	from	wastewater	112:121	arg1	removal					88:94	heavy metal Cr (VI) removal	68:94	heavy metal Cr (VI) removal from industrial wastewater	68:121	Development of high-capacity surface-engineered MXene composite for heavy metal Cr (VI) removal from industrial wastewater.					
36940825	3	105	theme	inexpensive	455:465	arg1	It					435:436	It	435:436	It	435:436	It is also the most inexpensive, lightweight, and globally friendly material tested.					
36940825	3	105	theme	inexpensive	455:465	arg1	material					503:510	the most inexpensive, lightweight, and globally friendly material	446:510	the most inexpensive, lightweight, and globally friendly material tested	446:517	It is also the most inexpensive, lightweight, and globally friendly material tested.					
37300397	7	0	theme	residual	1402:1409	arg1	trastuzumab					1420:1430	residual, unbound trastuzumab	1402:1430	residual, unbound trastuzumab	1402:1430	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	2	1	theme	mAbs	391:394	arg1	delivery					375:382	sustained and localized delivery	351:382	sustained and localized delivery of the mAbs	351:394	Macroporous hydrogels and affinity-based strategies have shown promising results in sustained and localized delivery of the mAbs.					
37300397	7	2	dep	affinity-controlled	1469:1487	arg1	slow-rate					1490:1498	slow-rate	1490:1498	slow-rate	1490:1498	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	6	3	theme	length	1043:1048	arg1	influence					1018:1026	the influence	1014:1026	the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner)	1014:1240	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	5	4	from	C-termini	814:822	arg1	addition					782:789	addition	782:789	addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both)	782:883	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	7	5	attach	released	1286:1293	arg2	antibodies					1271:1280	antibodies	1271:1280	antibodies	1271:1280	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	7	5	attach	released	1286:1293	arg1	hydrogels					1316:1324	the macroporous hydrogels	1300:1324	the macroporous hydrogels	1300:1324	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	3	6	theme	high-affinity	531:543	arg1	complex					572:578	a high-affinity, heterodimeric coiled-coil complex	529:578	complex	572:578	Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions.					
37300397	0	7	theme	coiled-coil	115:125	arg1	interactions					127:138	coiled-coil interactions	115:138	coiled-coil interactions	115:138	Affinity-controlled capture and release of engineered monoclonal antibodies by macroporous dextran hydrogels using coiled-coil interactions.					
37300397	5	8	dep	chains	840:845	arg1	chains					868:873	heavy chains	862:873	heavy chains	862:873	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	5	8	dep	chains	840:845	arg1	both					879:882	both	879:882	both	879:882	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	5	8	dep	chains	840:845	arg1	chains					854:859	light chains	848:859	light chains	848:859	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	5	9	from	production	905:914	arg1	cells					947:951	CHO cells	943:951	CHO cells	943:951	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	5	10	theme	antibody	963:970	arg1	binding					972:978	antibody binding	963:978	antibody binding to its antigen	963:993	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	7	11	theme	Kcoil-functionalized	1531:1550	arg1	surface					1562:1568	the Kcoil-functionalized macropore surface	1527:1568	the Kcoil-functionalized macropore surface	1527:1568	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	5	12	theme	CHO	943:945	arg1	cells					947:951	CHO cells	943:951	CHO cells	943:951	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	7	13	theme	antibodies	1511:1520	arg1	release					1500:1506	the affinity-controlled, slow-rate release	1465:1506	the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface	1465:1568	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	6	14	theme	Ecoil	1071:1075	arg1	tags					1077:1080	the Ecoil tags	1067:1080	the Ecoil tags	1067:1080	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	3	15	theme	physiological	586:598	arg1	conditions					600:609	physiological conditions	586:609	physiological conditions	586:609	Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions.					
37300397	4	16	theme	various	681:687	arg1	peptides					695:702	various Ecoil peptides	681:702	various Ecoil peptides	681:702	In this study, we created a set of trastuzumab molecules tagged with various Ecoil peptides and evaluated their manufacturability and characteristics.					
37300397	5	17	theme	heavy	862:866	arg1	chains					868:873	heavy chains	862:873	heavy chains	862:873	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	4	18	theme	molecules	659:667	arg1	set					640:642	a set	638:642	a set of trastuzumab molecules tagged with various Ecoil peptides	638:702	In this study, we created a set of trastuzumab molecules tagged with various Ecoil peptides and evaluated their manufacturability and characteristics.					
37300397	4	18	theme	molecules	659:667	arg1	molecules					659:667	trastuzumab molecules	647:667	trastuzumab molecules tagged with various Ecoil peptides	647:702	In this study, we created a set of trastuzumab molecules tagged with various Ecoil peptides and evaluated their manufacturability and characteristics.					
37300397	3	19	theme	coiled-coil	560:570	arg1	complex					572:578	a high-affinity, heterodimeric coiled-coil complex	529:578	complex	572:578	Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions.					
37300397	0	20	theme	monoclonal	54:63	arg1	antibodies					65:74	engineered monoclonal antibodies	43:74	engineered monoclonal antibodies	43:74	Affinity-controlled capture and release of engineered monoclonal antibodies by macroporous dextran hydrogels using coiled-coil interactions.					
37300397	7	21	theme	trastuzumab	1420:1430	arg1	release					1391:1397	the rapid release	1381:1397	the rapid release of residual, unbound trastuzumab from the macropores	1381:1450	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	2	22	theme	affinity-based	293:306	arg1	strategies					308:317	affinity-based strategies	293:317	affinity-based strategies	293:317	Macroporous hydrogels and affinity-based strategies have shown promising results in sustained and localized delivery of the mAbs.					
37300397	3	23	theme	designed	472:479	arg1	peptides					497:504	the de novo designed Ecoil and Kcoil peptides	460:504	the de novo designed Ecoil and Kcoil peptides	460:504	Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions.					
37300397	5	24	theme	chains	840:845	arg1	C-termini					814:822	the C-termini	810:822	the C-termini of the antibody chains (light chains, heavy chains, or both)	810:883	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	5	25	theme	Ecoil	797:801	arg1	tag					803:805	an Ecoil tag	794:805	an Ecoil tag	794:805	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	2	26	theme	sustained	351:359	arg1	delivery					375:382	sustained and localized delivery	351:382	sustained and localized delivery of the mAbs	351:394	Macroporous hydrogels and affinity-based strategies have shown promising results in sustained and localized delivery of the mAbs.					
37300397	6	27	from	hydrogels	1162:1170	arg1	capture					1089:1095	the capture and release	1085:1107	capture	1089:1095	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	6	27	from	hydrogels	1162:1170	arg1	release					1101:1107	the capture and release	1085:1107	release	1101:1107	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	1	28	theme	adverse	204:210	arg1	effects					212:218	the adverse effects	200:218	the adverse effects of monoclonal antibody (mAb)-based treatments	200:264	Long-term delivery is a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments.					
37300397	0	29	theme	Affinity-controlled	0:18	arg1	capture					20:26	Affinity-controlled capture	0:26	Affinity-controlled capture	0:26	Affinity-controlled capture and release of engineered monoclonal antibodies by macroporous dextran hydrogels using coiled-coil interactions.					
37300397	7	30	from	macropores	1441:1450	arg1	release					1391:1397	the rapid release	1381:1397	the rapid release of residual, unbound trastuzumab from the macropores	1381:1450	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	6	31	theme	trastuzumab	1125:1135	arg1	capture					1089:1095	the capture and release	1085:1107	capture	1089:1095	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	6	31	theme	trastuzumab	1125:1135	arg1	release					1101:1107	the capture and release	1085:1107	release	1101:1107	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	3	32	theme	de	464:465	arg1	peptides					497:504	the de novo designed Ecoil and Kcoil peptides	460:504	the de novo designed Ecoil and Kcoil peptides	460:504	Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions.					
37300397	5	33	theme	light	848:852	arg1	chains					854:859	light chains	848:859	light chains	848:859	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	6	34	theme	Kcoil	1192:1196	arg1	peptide					1198:1204	Kcoil peptide	1192:1204	Kcoil peptide (the Ecoil peptide-binding partner)	1192:1240	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	6	34	theme	Kcoil	1192:1196	arg1	partner					1233:1239	the Ecoil peptide-binding partner	1207:1239	the Ecoil peptide-binding partner	1207:1239	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	7	35	theme	macropore	1552:1560	arg1	surface					1562:1568	the Kcoil-functionalized macropore surface	1527:1568	the Kcoil-functionalized macropore surface	1527:1568	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	6	36	theme	number	1035:1040	arg1	influence					1018:1026	the influence	1014:1026	the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner)	1014:1240	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	6	37	theme	position	1055:1062	arg1	influence					1018:1026	the influence	1014:1026	the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner)	1014:1240	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	3	38	theme	Kcoil	491:495	arg1	peptides					497:504	the de novo designed Ecoil and Kcoil peptides	460:504	the de novo designed Ecoil and Kcoil peptides	460:504	Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions.					
37300397	3	39	theme	potential	407:415	arg1	tools					417:421	the potential tools	403:421	the potential tools for affinity-based delivery systems	403:457	Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions.					
37300397	6	40	theme	dextran	1154:1160	arg1	hydrogels					1162:1170	macroporous dextran hydrogels	1142:1170	macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner)	1142:1240	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	2	41	theme	promising	330:338	arg1	results					340:346	promising results	330:346	promising results	330:346	Macroporous hydrogels and affinity-based strategies have shown promising results in sustained and localized delivery of the mAbs.					
37300397	7	42	theme	rapid	1385:1389	arg1	release					1391:1397	the rapid release	1381:1397	the rapid release of residual, unbound trastuzumab from the macropores	1381:1450	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	1	43	theme	monoclonal	223:232	arg1	mAb					244:246	mAb	244:246	mAb	244:246	Long-term delivery is a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments.					
37300397	1	43	theme	monoclonal	223:232	arg1	antibody					234:241	monoclonal antibody	223:241	monoclonal antibody (mAb)-based treatments	223:264	Long-term delivery is a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments.					
37300397	0	44	theme	engineered	43:52	arg1	antibodies					65:74	engineered monoclonal antibodies	43:74	engineered monoclonal antibodies	43:74	Affinity-controlled capture and release of engineered monoclonal antibodies by macroporous dextran hydrogels using coiled-coil interactions.					
37300397	7	45	dep	residual	1402:1409	arg1	unbound					1412:1418	unbound	1412:1418	unbound	1412:1418	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	7	46	from	surface	1562:1568	arg1	release					1500:1506	the affinity-controlled, slow-rate release	1465:1506	the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface	1465:1568	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	1	47	theme	antibody	234:241	arg1	treatments					255:264	monoclonal antibody (mAb)-based treatments	223:264	monoclonal antibody (mAb)-based treatments	223:264	Long-term delivery is a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments.					
37300397	7	48	theme	macroporous	1304:1314	arg1	hydrogels					1316:1324	the macroporous hydrogels	1300:1324	the macroporous hydrogels	1300:1324	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	3	49	theme	Ecoil	481:485	arg1	peptides					497:504	the de novo designed Ecoil and Kcoil peptides	460:504	the de novo designed Ecoil and Kcoil peptides	460:504	Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions.					
37300397	3	50	dep	de	464:465	arg1	novo					467:470	novo	467:470	novo	467:470	Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions.					
37300397	0	51	theme	antibodies	65:74	arg1	release					32:38	release	32:38	release	32:38	Affinity-controlled capture and release of engineered monoclonal antibodies by macroporous dextran hydrogels using coiled-coil interactions.					
37300397	0	51	theme	antibodies	65:74	arg1	capture					20:26	Affinity-controlled capture	0:26	Affinity-controlled capture	0:26	Affinity-controlled capture and release of engineered monoclonal antibodies by macroporous dextran hydrogels using coiled-coil interactions.					
37300397	5	52	theme	antibody	831:838	arg1	chains					840:845	the antibody chains	827:845	the antibody chains (light chains, heavy chains, or both)	827:883	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	5	53	theme	tag	803:805	arg1	addition					782:789	addition	782:789	addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both)	782:883	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	6	54	theme	Ecoil	1211:1215	arg1	peptide					1198:1204	Kcoil peptide	1192:1204	Kcoil peptide (the Ecoil peptide-binding partner)	1192:1240	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	6	54	theme	Ecoil	1211:1215	arg1	partner					1233:1239	the Ecoil peptide-binding partner	1207:1239	the Ecoil peptide-binding partner	1207:1239	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	1	55	theme	Long-term	141:149	arg1	strategy					176:183	a successful strategy	163:183	a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments	163:264	Long-term delivery is a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments.					
37300397	1	55	theme	Long-term	141:149	arg1	delivery					151:158	Long-term delivery	141:158	Long-term delivery	141:158	Long-term delivery is a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments.					
37300397	3	56	theme	delivery	442:449	arg1	systems					451:457	affinity-based delivery systems	427:457	affinity-based delivery systems	427:457	Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions.					
37300397	7	57	theme	first	1352:1356	arg1	phase					1358:1362	the first phase	1348:1362	the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores	1348:1450	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	5	58	theme	chimeric	919:926	arg1	trastuzumab					928:938	chimeric trastuzumab	919:938	chimeric trastuzumab	919:938	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	7	59	theme	biphasic	1331:1338	arg1	manner					1340:1345	a biphasic manner	1329:1345	a biphasic manner	1329:1345	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	6	60	theme	peptide-binding	1217:1231	arg1	peptide					1198:1204	Kcoil peptide	1192:1204	Kcoil peptide (the Ecoil peptide-binding partner)	1192:1240	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	6	60	theme	peptide-binding	1217:1231	arg1	partner					1233:1239	the Ecoil peptide-binding partner	1207:1239	the Ecoil peptide-binding partner	1207:1239	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	2	61	theme	localized	365:373	arg1	delivery					375:382	sustained and localized delivery	351:382	sustained and localized delivery of the mAbs	351:394	Macroporous hydrogels and affinity-based strategies have shown promising results in sustained and localized delivery of the mAbs.					
37300397	6	62	theme	Ecoil-tagged	1112:1123	arg1	trastuzumab					1125:1135	Ecoil-tagged trastuzumab	1112:1135	Ecoil-tagged trastuzumab	1112:1135	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	6	63	theme	macroporous	1142:1152	arg1	hydrogels					1162:1170	macroporous dextran hydrogels	1142:1170	macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner)	1142:1240	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	7	64	theme	affinity-controlled	1469:1487	arg1	release					1500:1506	the affinity-controlled, slow-rate release	1465:1506	the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface	1465:1568	Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface.					
37300397	6	65	dep	number	1035:1040	arg1	the					1031:1033	the	1031:1033	the	1031:1033	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	6	66	theme	tags	1077:1080	arg1	length					1043:1048	length	1043:1048	length	1043:1048	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	6	66	theme	tags	1077:1080	arg1	position					1055:1062	position	1055:1062	position	1055:1062	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	6	66	theme	tags	1077:1080	arg1	number					1035:1040	number	1035:1040	number	1035:1040	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	5	67	theme	trastuzumab	928:938	arg1	production					905:914	the production	901:914	the production of chimeric trastuzumab in CHO cells	901:951	Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen.					
37300397	0	68	theme	macroporous	79:89	arg1	dextran					91:97	macroporous dextran	79:97	macroporous dextran	79:97	Affinity-controlled capture and release of engineered monoclonal antibodies by macroporous dextran hydrogels using coiled-coil interactions.					
37300397	2	69	theme	Macroporous	267:277	arg1	hydrogels					279:287	Macroporous hydrogels	267:287	Macroporous hydrogels	267:287	Macroporous hydrogels and affinity-based strategies have shown promising results in sustained and localized delivery of the mAbs.					
37300397	4	70	theme	trastuzumab	647:657	arg1	molecules					659:667	trastuzumab molecules	647:667	trastuzumab molecules tagged with various Ecoil peptides	647:702	In this study, we created a set of trastuzumab molecules tagged with various Ecoil peptides and evaluated their manufacturability and characteristics.					
37300397	1	71	theme	successful	165:174	arg1	strategy					176:183	a successful strategy	163:183	a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments	163:264	Long-term delivery is a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments.					
37300397	1	71	theme	successful	165:174	arg1	delivery					151:158	Long-term delivery	141:158	Long-term delivery	141:158	Long-term delivery is a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments.					
37300397	4	72	theme	Ecoil	689:693	arg1	peptides					695:702	various Ecoil peptides	681:702	various Ecoil peptides	681:702	In this study, we created a set of trastuzumab molecules tagged with various Ecoil peptides and evaluated their manufacturability and characteristics.					
37300397	1	73	theme	-based	248:253	arg1	treatments					255:264	monoclonal antibody (mAb)-based treatments	223:264	monoclonal antibody (mAb)-based treatments	223:264	Long-term delivery is a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments.					
37300397	3	74	theme	affinity-based	427:440	arg1	systems					451:457	affinity-based delivery systems	427:457	affinity-based delivery systems	427:457	Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions.					
37300397	6	75	from	influence	1018:1026	arg1	capture					1089:1095	the capture and release	1085:1107	capture	1089:1095	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	6	75	from	influence	1018:1026	arg1	release					1101:1107	the capture and release	1085:1107	release	1101:1107	We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner).					
37300397	3	76	theme	heterodimeric	546:558	arg1	complex					572:578	a high-affinity, heterodimeric coiled-coil complex	529:578	complex	572:578	Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions.					
37300397	1	77	theme	treatments	255:264	arg1	effects					212:218	the adverse effects	200:218	the adverse effects of monoclonal antibody (mAb)-based treatments	200:264	Long-term delivery is a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments.					
35429899	7	0	theme	minimum	1137:1143	arg1	MIC					1171:1173	MIC	1171:1173	MIC	1171:1173	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	7	0	theme	minimum	1137:1143	arg1	concentration					1156:1168	The minimum inhibitory concentration	1133:1168	The minimum inhibitory concentration (MIC)	1133:1174	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	7	0	theme	minimum	1137:1143	arg1	25 mg·mL-1					1295:1304	25 mg·mL-1	1295:1304	25 mg·mL-1	1295:1304	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	4	1	theme	@	687:687	arg1	U-CD-MOF					688:695	CA@U-CD-MOF	685:695	CA@U-CD-MOF	685:695	U-CD-MOF was used to load the antibacterial natural product CA to form the composite (CA@U-CD-MOF) and the loading rate of CA@U-CD-MOF to CA could reach 19.63 ± 2.53%, which was more than twice that of γ-CD.					
35429899	4	1	theme	@	687:687	arg1	composite					674:682	the composite	670:682	the composite (CA@U-CD-MOF)	670:696	U-CD-MOF was used to load the antibacterial natural product CA to form the composite (CA@U-CD-MOF) and the loading rate of CA@U-CD-MOF to CA could reach 19.63 ± 2.53%, which was more than twice that of γ-CD.					
35429899	7	2	dep	H7	1263:1264	arg1	O157					1275:1278	E. coli O157	1267:1278	E. coli O157: H7	1267:1282	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	9	3	theme	release	1388:1394	arg1	behavior					1396:1403	The sustained release behavior	1374:1403	The sustained release behavior of CA@U-CD-MOF to CA in ethanol	1374:1435	The sustained release behavior of CA@U-CD-MOF to CA in ethanol fitted well to Higuchi model and the loading of CA was supported by molecular docking results.					
35429899	9	4	from	U-CD-MOF	1411:1418	arg1	ethanol					1429:1435	ethanol	1429:1435	ethanol	1429:1435	The sustained release behavior of CA@U-CD-MOF to CA in ethanol fitted well to Higuchi model and the loading of CA was supported by molecular docking results.					
35429899	7	5	theme	inhibitory	1145:1154	arg1	MIC					1171:1173	MIC	1171:1173	MIC	1171:1173	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	7	5	theme	inhibitory	1145:1154	arg1	concentration					1156:1168	The minimum inhibitory concentration	1133:1168	The minimum inhibitory concentration (MIC)	1133:1174	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	7	5	theme	inhibitory	1145:1154	arg1	25 mg·mL-1					1295:1304	25 mg·mL-1	1295:1304	25 mg·mL-1	1295:1304	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	3	6	contain	had	540:542	arg1	U-CD-MOF					531:538	U-CD-MOF	531:538	U-CD-MOF	531:538	Under the optimal conditions, U-CD-MOF had a cubic structure with uniform size of 8.60 ± 1.95 μm.					
35429899	3	6	contain	had	540:542	arg2	structure					552:560	a cubic structure	544:560	a cubic structure with uniform size of 8.60 ± 1.95 μm	544:596	Under the optimal conditions, U-CD-MOF had a cubic structure with uniform size of 8.60 ± 1.95 μm.					
35429899	9	7	theme	CA	1485:1486	arg1	loading					1474:1480	the loading	1470:1480	the loading of CA	1470:1486	The sustained release behavior of CA@U-CD-MOF to CA in ethanol fitted well to Higuchi model and the loading of CA was supported by molecular docking results.					
35429899	1	8	theme	caffeic	212:218	arg1	loading					230:236	caffeic acid (CA) loading	212:236	caffeic acid (CA) loading	212:236	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF) was successfully studied and this method shortened the preparation time to a few minutes.					
35429899	5	9	theme	X-ray	897:901	arg1	PXRD					916:919	PXRD	916:919	PXRD	916:919	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	5	9	theme	X-ray	897:901	arg1	diffraction					903:913	Powder X-ray diffraction	890:913	Powder X-ray diffraction (PXRD)	890:920	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	10	10	theme	potential	1669:1677	arg1	application					1693:1703	potential antibacterial application	1669:1703	potential antibacterial application	1669:1703	In general, U-CD-MOF was successfully achieved by ultrasound-assisted rapid synthesis and the obtained crystal was further evaluated for potential antibacterial application.					
35429899	7	11	theme	Escherichia	1240:1250	arg1	O157					1257:1260	Escherichia coli O157	1240:1260	Escherichia coli O157: H7 (E. coli O157: H7)	1240:1283	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	5	12	theme	scanning	971:978	arg1	SEM					1001:1003	SEM	1001:1003	SEM	1001:1003	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	5	12	theme	scanning	971:978	arg1	microscopy					989:998	scanning electron microscopy	971:998	scanning electron microscopy (SEM)	971:1004	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	7	13	theme	@	1226:1226	arg1	U-CD-MOF					1227:1234	CA@U-CD-MOF	1224:1234	CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7)	1224:1283	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	4	14	theme	@	724:724	arg1	U-CD-MOF					725:732	CA@U-CD-MOF	722:732	CA@U-CD-MOF	722:732	U-CD-MOF was used to load the antibacterial natural product CA to form the composite (CA@U-CD-MOF) and the loading rate of CA@U-CD-MOF to CA could reach 19.63 ± 2.53%, which was more than twice that of γ-CD.					
35429899	7	15	dep	Escherichia	1240:1250	arg1	coli					1252:1255	coli	1252:1255	coli	1252:1255	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	0	16	theme	antibacterial	105:117	arg1	application					119:129	antibacterial application	105:129	antibacterial application	105:129	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid loading and antibacterial application.					
35429899	10	17	theme	obtained	1626:1633	arg1	crystal					1635:1641	the obtained crystal	1622:1641	the obtained crystal	1622:1641	In general, U-CD-MOF was successfully achieved by ultrasound-assisted rapid synthesis and the obtained crystal was further evaluated for potential antibacterial application.					
35429899	10	18	theme	rapid	1602:1606	arg1	synthesis					1608:1616	ultrasound-assisted rapid synthesis	1582:1616	ultrasound-assisted rapid synthesis	1582:1616	In general, U-CD-MOF was successfully achieved by ultrasound-assisted rapid synthesis and the obtained crystal was further evaluated for potential antibacterial application.					
35429899	9	19	theme	CA	1408:1409	arg1	U-CD-MOF					1411:1418	CA@U-CD-MOF	1408:1418	CA@U-CD-MOF to CA in ethanol	1408:1435	The sustained release behavior of CA@U-CD-MOF to CA in ethanol fitted well to Higuchi model and the loading of CA was supported by molecular docking results.					
35429899	4	20	theme	loading	706:712	arg1	rate					714:717	the loading rate	702:717	the loading rate of CA@U-CD-MOF to CA	702:738	U-CD-MOF was used to load the antibacterial natural product CA to form the composite (CA@U-CD-MOF) and the loading rate of CA@U-CD-MOF to CA could reach 19.63 ± 2.53%, which was more than twice that of γ-CD.					
35429899	5	21	dep	Fourier	923:929	arg1	transform					931:939	transform	931:939	transform infrared spectroscopy (FTIR)	931:968	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	2	22	dep	morphology	456:465	arg1	the					452:454	the	452:454	the	452:454	It was found that the ultrasonic power, reaction time and temperature would affect the morphology and size of the obtained crystal.					
35429899	4	23	theme	natural	643:649	arg1	product					651:657	the antibacterial natural product CA	625:660	the antibacterial natural product CA	625:660	U-CD-MOF was used to load the antibacterial natural product CA to form the composite (CA@U-CD-MOF) and the loading rate of CA@U-CD-MOF to CA could reach 19.63 ± 2.53%, which was more than twice that of γ-CD.					
35429899	9	24	theme	@	1410:1410	arg1	U-CD-MOF					1411:1418	CA@U-CD-MOF	1408:1418	CA@U-CD-MOF to CA in ethanol	1408:1435	The sustained release behavior of CA@U-CD-MOF to CA in ethanol fitted well to Higuchi model and the loading of CA was supported by molecular docking results.					
35429899	7	25	theme	U-CD-MOF	1227:1234	arg1	concentration					1201:1213	minimum bactericidal concentration	1180:1213	minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7)	1180:1283	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	7	25	theme	U-CD-MOF	1227:1234	arg1	MIC					1171:1173	MIC	1171:1173	MIC	1171:1173	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	7	25	theme	U-CD-MOF	1227:1234	arg1	concentration					1156:1168	The minimum inhibitory concentration	1133:1168	The minimum inhibitory concentration (MIC)	1133:1174	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	7	25	theme	U-CD-MOF	1227:1234	arg1	25 mg·mL-1					1295:1304	25 mg·mL-1	1295:1304	25 mg·mL-1	1295:1304	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	7	25	theme	U-CD-MOF	1227:1234	arg1	MBC					1216:1218	MBC	1216:1218	MBC	1216:1218	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	1	26	theme	acid	220:223	arg1	loading					230:236	caffeic acid (CA) loading	212:236	caffeic acid (CA) loading	212:236	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF) was successfully studied and this method shortened the preparation time to a few minutes.					
35429899	5	27	theme	synthesized	859:869	arg1	crystal					871:877	the synthesized crystal	855:877	the synthesized crystal	855:877	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	0	28	theme	rapid	60:64	arg1	synthesis					66:74	ultrasound-assisted rapid synthesis	40:74	ultrasound-assisted rapid synthesis for caffeic acid loading and antibacterial application	40:129	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid loading and antibacterial application.					
35429899	3	29	theme	cubic	546:550	arg1	structure					552:560	a cubic structure	544:560	a cubic structure with uniform size of 8.60 ± 1.95 μm	544:596	Under the optimal conditions, U-CD-MOF had a cubic structure with uniform size of 8.60 ± 1.95 μm.					
35429899	2	30	theme	reaction	409:416	arg1	time					418:421	reaction time	409:421	reaction time	409:421	It was found that the ultrasonic power, reaction time and temperature would affect the morphology and size of the obtained crystal.					
35429899	9	31	theme	docking	1515:1521	arg1	results					1523:1529	molecular docking results	1505:1529	molecular docking results	1505:1529	The sustained release behavior of CA@U-CD-MOF to CA in ethanol fitted well to Higuchi model and the loading of CA was supported by molecular docking results.					
35429899	7	32	theme	minimum	1180:1186	arg1	concentration					1201:1213	minimum bactericidal concentration	1180:1213	minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7)	1180:1283	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	7	32	theme	minimum	1180:1186	arg1	MBC					1216:1218	MBC	1216:1218	MBC	1216:1218	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	4	33	theme	CA	722:723	arg1	U-CD-MOF					725:732	CA@U-CD-MOF	722:732	CA@U-CD-MOF	722:732	U-CD-MOF was used to load the antibacterial natural product CA to form the composite (CA@U-CD-MOF) and the loading rate of CA@U-CD-MOF to CA could reach 19.63 ± 2.53%, which was more than twice that of γ-CD.					
35429899	1	34	theme	CA	226:227	arg1	loading					230:236	caffeic acid (CA) loading	212:236	caffeic acid (CA) loading	212:236	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF) was successfully studied and this method shortened the preparation time to a few minutes.					
35429899	7	35	dep	O157	1275:1278	arg1	H7					1281:1282	H7	1281:1282	E. coli O157: H7	1267:1282	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	5	36	theme	Various	807:813	arg1	techniques					815:824	Various techniques	807:824	Various techniques	807:824	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	3	37	theme	8.60 ± 1.95 μm	583:596	arg1	size					575:578	uniform size	567:578	uniform size of 8.60 ± 1.95 μm	567:596	Under the optimal conditions, U-CD-MOF had a cubic structure with uniform size of 8.60 ± 1.95 μm.					
35429899	9	38	theme	sustained	1378:1386	arg1	behavior					1396:1403	The sustained release behavior	1374:1403	The sustained release behavior of CA@U-CD-MOF to CA in ethanol	1374:1435	The sustained release behavior of CA@U-CD-MOF to CA in ethanol fitted well to Higuchi model and the loading of CA was supported by molecular docking results.					
35429899	7	39	theme	E.	1267:1268	arg1	O157					1275:1278	E. coli O157	1267:1278	E. coli O157: H7	1267:1282	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	5	40	theme	Powder	890:895	arg1	PXRD					916:919	PXRD	916:919	PXRD	916:919	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	5	40	theme	Powder	890:895	arg1	diffraction					903:913	Powder X-ray diffraction	890:913	Powder X-ray diffraction (PXRD)	890:920	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	3	41	theme	uniform	567:573	arg1	size					575:578	uniform size	567:578	uniform size of 8.60 ± 1.95 μm	567:596	Under the optimal conditions, U-CD-MOF had a cubic structure with uniform size of 8.60 ± 1.95 μm.					
35429899	7	42	theme	CA	1224:1225	arg1	U-CD-MOF					1227:1234	CA@U-CD-MOF	1224:1234	CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7)	1224:1283	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	6	43	theme	antibacterial	1073:1085	arg1	tests					1087:1091	antibacterial tests	1073:1091	antibacterial tests	1073:1091	In addition, antibacterial tests were performed on the obtained crystal.					
35429899	10	44	theme	ultrasound-assisted	1582:1600	arg1	synthesis					1608:1616	ultrasound-assisted rapid synthesis	1582:1616	ultrasound-assisted rapid synthesis	1582:1616	In general, U-CD-MOF was successfully achieved by ultrasound-assisted rapid synthesis and the obtained crystal was further evaluated for potential antibacterial application.					
35429899	9	45	theme	molecular	1505:1513	arg1	results					1523:1529	molecular docking results	1505:1529	molecular docking results	1505:1529	The sustained release behavior of CA@U-CD-MOF to CA in ethanol fitted well to Higuchi model and the loading of CA was supported by molecular docking results.					
35429899	1	46	theme	Cyclodextrin	132:143	arg1	framework					159:167	Cyclodextrin metal-organic framework	132:167	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF)	132:277	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF) was successfully studied and this method shortened the preparation time to a few minutes.					
35429899	1	47	theme	rapid	192:196	arg1	synthesis					198:206	ultrasound-assisted rapid synthesis	172:206	ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF)	172:277	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF) was successfully studied and this method shortened the preparation time to a few minutes.					
35429899	2	48	theme	crystal	492:498	arg1	size					471:474	size	471:474	size	471:474	It was found that the ultrasonic power, reaction time and temperature would affect the morphology and size of the obtained crystal.					
35429899	2	48	theme	crystal	492:498	arg1	morphology					456:465	morphology	456:465	morphology	456:465	It was found that the ultrasonic power, reaction time and temperature would affect the morphology and size of the obtained crystal.					
35429899	1	49	theme	preparation	334:344	arg1	time					346:349	the preparation time	330:349	the preparation time to a few minutes	330:366	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF) was successfully studied and this method shortened the preparation time to a few minutes.					
35429899	5	50	theme	thermogravimetric	1007:1023	arg1	TGA					1035:1037	TGA	1035:1037	TGA	1035:1037	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	5	50	theme	thermogravimetric	1007:1023	arg1	analysis					1025:1032	thermogravimetric analysis	1007:1032	thermogravimetric analysis (TGA)	1007:1038	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	1	51	theme	antibacterial	242:254	arg1	U-CD-MOF					269:276	U-CD-MOF	269:276	U-CD-MOF	269:276	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF) was successfully studied and this method shortened the preparation time to a few minutes.					
35429899	1	51	theme	antibacterial	242:254	arg1	application					256:266	antibacterial application	242:266	antibacterial application (U-CD-MOF)	242:277	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF) was successfully studied and this method shortened the preparation time to a few minutes.					
35429899	2	52	theme	obtained	483:490	arg1	crystal					492:498	the obtained crystal	479:498	the obtained crystal	479:498	It was found that the ultrasonic power, reaction time and temperature would affect the morphology and size of the obtained crystal.					
35429899	0	53	theme	ultrasound-assisted	40:58	arg1	synthesis					66:74	ultrasound-assisted rapid synthesis	40:74	ultrasound-assisted rapid synthesis for caffeic acid loading and antibacterial application	40:129	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid loading and antibacterial application.					
35429899	7	54	dep	aureus	1338:1343	arg1	aureus					1349:1354	S. aureus	1346:1354	S. aureus	1346:1354	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	3	55	with	structure	552:560	arg1	size					575:578	uniform size	567:578	uniform size of 8.60 ± 1.95 μm	567:596	Under the optimal conditions, U-CD-MOF had a cubic structure with uniform size of 8.60 ± 1.95 μm.					
35429899	4	56	dep	product	651:657	arg1	CA					659:660	CA	659:660	the antibacterial natural product CA	625:660	U-CD-MOF was used to load the antibacterial natural product CA to form the composite (CA@U-CD-MOF) and the loading rate of CA@U-CD-MOF to CA could reach 19.63 ± 2.53%, which was more than twice that of γ-CD.					
35429899	1	57	theme	metal-organic	145:157	arg1	framework					159:167	Cyclodextrin metal-organic framework	132:167	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF)	132:277	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF) was successfully studied and this method shortened the preparation time to a few minutes.					
35429899	4	58	used	used	612:615	arg2	U-CD-MOF					599:606	U-CD-MOF	599:606	U-CD-MOF	599:606	U-CD-MOF was used to load the antibacterial natural product CA to form the composite (CA@U-CD-MOF) and the loading rate of CA@U-CD-MOF to CA could reach 19.63 ± 2.53%, which was more than twice that of γ-CD.					
35429899	5	59	theme	electron	980:987	arg1	SEM					1001:1003	SEM	1001:1003	SEM	1001:1003	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	5	59	theme	electron	980:987	arg1	microscopy					989:998	scanning electron microscopy	971:998	scanning electron microscopy (SEM)	971:1004	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	3	60	theme	optimal	511:517	arg1	conditions					519:528	the optimal conditions	507:528	the optimal conditions	507:528	Under the optimal conditions, U-CD-MOF had a cubic structure with uniform size of 8.60 ± 1.95 μm.					
35429899	1	61	theme	few	356:358	arg1	minutes					360:366	a few minutes	354:366	a few minutes	354:366	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF) was successfully studied and this method shortened the preparation time to a few minutes.					
35429899	7	62	dep	O157	1257:1260	arg1	H7					1263:1264	H7	1263:1264	Escherichia coli O157: H7 (E. coli O157: H7)	1240:1283	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	9	63	from	behavior	1396:1403	arg1	ethanol					1429:1435	ethanol	1429:1435	ethanol	1429:1435	The sustained release behavior of CA@U-CD-MOF to CA in ethanol fitted well to Higuchi model and the loading of CA was supported by molecular docking results.					
35429899	7	64	theme	bactericidal	1188:1199	arg1	concentration					1201:1213	minimum bactericidal concentration	1180:1213	minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7)	1180:1283	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	7	64	theme	bactericidal	1188:1199	arg1	MBC					1216:1218	MBC	1216:1218	MBC	1216:1218	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	4	65	theme	U-CD-MOF	725:732	arg1	rate					714:717	the loading rate	702:717	the loading rate of CA@U-CD-MOF to CA	702:738	U-CD-MOF was used to load the antibacterial natural product CA to form the composite (CA@U-CD-MOF) and the loading rate of CA@U-CD-MOF to CA could reach 19.63 ± 2.53%, which was more than twice that of γ-CD.					
35429899	7	66	dep	E.	1267:1268	arg1	coli					1270:1273	coli	1270:1273	coli	1270:1273	The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA@U-CD-MOF for Escherichia coli O157: H7 (E. coli O157: H7) were both 25 mg·mL-1, and the MIC for Staphylococcus aureus (S. aureus).					
35429899	9	67	theme	U-CD-MOF	1411:1418	arg1	behavior					1396:1403	The sustained release behavior	1374:1403	The sustained release behavior of CA@U-CD-MOF to CA in ethanol	1374:1435	The sustained release behavior of CA@U-CD-MOF to CA in ethanol fitted well to Higuchi model and the loading of CA was supported by molecular docking results.					
35429899	1	68	theme	ultrasound-assisted	172:190	arg1	synthesis					198:206	ultrasound-assisted rapid synthesis	172:206	ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF)	172:277	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid (CA) loading and antibacterial application (U-CD-MOF) was successfully studied and this method shortened the preparation time to a few minutes.					
35429899	0	69	theme	acid	88:91	arg1	loading					93:99	caffeic acid loading	80:99	caffeic acid loading	80:99	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid loading and antibacterial application.					
35429899	10	70	theme	antibacterial	1679:1691	arg1	application					1693:1703	potential antibacterial application	1669:1703	potential antibacterial application	1669:1703	In general, U-CD-MOF was successfully achieved by ultrasound-assisted rapid synthesis and the obtained crystal was further evaluated for potential antibacterial application.					
35429899	5	71	dep	transform	931:939	arg1	infrared					941:948	infrared	941:948	transform infrared spectroscopy (FTIR)	931:968	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	2	72	theme	ultrasonic	391:400	arg1	power					402:406	the ultrasonic power	387:406	the ultrasonic power	387:406	It was found that the ultrasonic power, reaction time and temperature would affect the morphology and size of the obtained crystal.					
35429899	9	73	from	ethanol	1429:1435	arg1	behavior					1396:1403	The sustained release behavior	1374:1403	The sustained release behavior of CA@U-CD-MOF to CA in ethanol	1374:1435	The sustained release behavior of CA@U-CD-MOF to CA in ethanol fitted well to Higuchi model and the loading of CA was supported by molecular docking results.					
35429899	4	74	theme	antibacterial	629:641	arg1	product					651:657	the antibacterial natural product CA	625:660	the antibacterial natural product CA	625:660	U-CD-MOF was used to load the antibacterial natural product CA to form the composite (CA@U-CD-MOF) and the loading rate of CA@U-CD-MOF to CA could reach 19.63 ± 2.53%, which was more than twice that of γ-CD.					
35429899	0	75	theme	caffeic	80:86	arg1	loading					93:99	caffeic acid loading	80:99	caffeic acid loading	80:99	Cyclodextrin metal-organic framework by ultrasound-assisted rapid synthesis for caffeic acid loading and antibacterial application.					
35429899	5	76	theme	N2	1045:1046	arg1	adsorption					1048:1057	N2 adsorption	1045:1057	N2 adsorption	1045:1057	Various techniques were applied to characterize the synthesized crystal, including Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2 adsorption.					
35429899	6	77	theme	obtained	1115:1122	arg1	crystal					1124:1130	the obtained crystal	1111:1130	the obtained crystal	1111:1130	In addition, antibacterial tests were performed on the obtained crystal.					
35429899	4	78	theme	CA	685:686	arg1	U-CD-MOF					688:695	CA@U-CD-MOF	685:695	CA@U-CD-MOF	685:695	U-CD-MOF was used to load the antibacterial natural product CA to form the composite (CA@U-CD-MOF) and the loading rate of CA@U-CD-MOF to CA could reach 19.63 ± 2.53%, which was more than twice that of γ-CD.					
35429899	4	78	theme	CA	685:686	arg1	composite					674:682	the composite	670:682	the composite (CA@U-CD-MOF)	670:696	U-CD-MOF was used to load the antibacterial natural product CA to form the composite (CA@U-CD-MOF) and the loading rate of CA@U-CD-MOF to CA could reach 19.63 ± 2.53%, which was more than twice that of γ-CD.					
35429899	9	79	theme	Higuchi	1452:1458	arg1	model					1460:1464	Higuchi model	1452:1464	Higuchi model	1452:1464	The sustained release behavior of CA@U-CD-MOF to CA in ethanol fitted well to Higuchi model and the loading of CA was supported by molecular docking results.					
36534030	6	0	theme	antioxidant	1289:1299	arg1	capacity					1301:1308	their antioxidant capacity	1283:1308	their antioxidant capacity	1283:1308	Although the phenolics and flavonoids content of JFP decreased with fruit maturity, their antioxidant capacity increased, which may be correlated with the increased content of galacturonic acid upon fruit development.					
36534030	3	1	theme	cell-wall	880:888	arg1	degradation					890:900	cell-wall degradation	880:900	cell-wall degradation during maturation	880:918	The molecular weight increased from 147 kDa in stage I to 169 kDa in stage III, but decreased to 114 kDa in stage IV, probably due to cell-wall degradation during maturation.					
36534030	1	2	theme	molecular	467:475	arg1	weight					477:482	molecular weight	467:482	molecular weight	467:482	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	2	3	dep	yield	567:571	arg1	increased					573:581	increased	573:581	increased	573:581	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	1	4	theme	functional	168:177	arg1	properties					179:188	The physicochemical and functional properties	144:188	The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV)	144:393	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	6	5	theme	JFP	1248:1250	arg1	content					1237:1243	the phenolics and flavonoids content	1208:1243	the phenolics and flavonoids content of JFP	1208:1250	Although the phenolics and flavonoids content of JFP decreased with fruit maturity, their antioxidant capacity increased, which may be correlated with the increased content of galacturonic acid upon fruit development.					
36534030	6	6	theme	phenolics	1212:1220	arg1	content					1237:1243	the phenolics and flavonoids content	1208:1243	the phenolics and flavonoids content of JFP	1208:1250	Although the phenolics and flavonoids content of JFP decreased with fruit maturity, their antioxidant capacity increased, which may be correlated with the increased content of galacturonic acid upon fruit development.					
36534030	1	7	theme	different	326:334	arg1	stages					345:350	four different maturity stages	321:350	four different maturity stages	321:350	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	9	8	theme	high-quality	1690:1701	arg1	pectin					1711:1716	high-quality gelling pectin	1690:1716	high-quality gelling pectin with antioxidant properties	1690:1744	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	4	9	theme	fruit	1023:1027	arg1	maturity					1029:1036	fruit maturity	1023:1036	fruit maturity	1023:1036	The JFP was of the high methoxyl type and the degree of esterification increased from 65% to 87% with fruit maturity.					
36534030	3	10	dep	stage	793:797	arg1	to					801:802	to	801:802	to	801:802	The molecular weight increased from 147 kDa in stage I to 169 kDa in stage III, but decreased to 114 kDa in stage IV, probably due to cell-wall degradation during maturation.					
36534030	0	11	theme	jackfruit	90:98	arg1	portions					78:85	the edible portions	67:85	the edible portions of jackfruit	67:98	Physicochemical and functional properties of pectin extracted from the edible portions of jackfruit at different stages of maturity.					
36534030	1	12	theme	maturity	336:343	arg1	stages					345:350	four different maturity stages	321:350	four different maturity stages	321:350	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	2	13	dep	content	675:681	arg1	%					695:695	57.1%	691:695	57.1%	691:695	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	2	13	dep	content	675:681	arg1	%					702:702	63.6%	698:702	63.6%	698:702	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	2	13	dep	content	675:681	arg1	%					713:713	65.2%	709:713	65.2%	709:713	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	2	13	dep	content	675:681	arg1	%					688:688	50.1%	684:688	50.1%	684:688	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	1	14	dep	BACKGROUND	133:142	arg1	characterized					400:412	characterized	400:412	were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods	395:549	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	1	15	theme	weight	477:482	arg1	terms					417:421	terms	417:421	terms of extraction yields, chemical composition, molecular weight, and antioxidant properties	417:510	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	3	16	theme	molecular	750:758	arg1	weight					760:765	The molecular weight	746:765	The molecular weight	746:765	The molecular weight increased from 147 kDa in stage I to 169 kDa in stage III, but decreased to 114 kDa in stage IV, probably due to cell-wall degradation during maturation.					
36534030	2	17	with	%	600:600	arg1	maturity					613:620	fruit maturity	607:620	fruit maturity	607:620	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	2	18	theme	acid	670:673	arg1	content					675:681	the galacturonic acid content	653:681	the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively	653:743	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	5	19	theme	functional	1043:1052	arg1	similar					1077:1083	similar	1077:1083	similar	1077:1083	The functional properties of JFP were similar to or better than those reported for commercial apple pectin, thus highlighting its potential as a food additive.					
36534030	5	19	theme	functional	1043:1052	arg1	properties					1054:1063	The functional properties	1039:1063	The functional properties of JFP	1039:1070	The functional properties of JFP were similar to or better than those reported for commercial apple pectin, thus highlighting its potential as a food additive.					
36534030	2	20	theme	galacturonic	657:668	arg1	content					675:681	the galacturonic acid content	653:681	the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively	653:743	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	0	21	theme	different	103:111	arg1	stages					113:118	different stages	103:118	different stages of maturity	103:130	Physicochemical and functional properties of pectin extracted from the edible portions of jackfruit at different stages of maturity.					
36534030	6	22	theme	galacturonic	1375:1386	arg1	acid					1388:1391	galacturonic acid	1375:1391	galacturonic acid	1375:1391	Although the phenolics and flavonoids content of JFP decreased with fruit maturity, their antioxidant capacity increased, which may be correlated with the increased content of galacturonic acid upon fruit development.					
36534030	6	23	theme	flavonoids	1226:1235	arg1	content					1237:1243	the phenolics and flavonoids content	1208:1243	the phenolics and flavonoids content of JFP	1208:1250	Although the phenolics and flavonoids content of JFP decreased with fruit maturity, their antioxidant capacity increased, which may be correlated with the increased content of galacturonic acid upon fruit development.					
36534030	5	24	theme	apple	1133:1137	arg1	pectin					1139:1144	commercial apple pectin	1122:1144	commercial apple pectin	1122:1144	The functional properties of JFP were similar to or better than those reported for commercial apple pectin, thus highlighting its potential as a food additive.					
36534030	2	25	dep	RESULT	552:557	arg1	yield					567:571	yield	567:571	yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively	567:743	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	1	26	theme	antioxidant	489:499	arg1	properties					501:510	antioxidant properties	489:510	antioxidant properties	489:510	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	1	27	theme	pectin	193:198	arg1	properties					179:188	The physicochemical and functional properties	144:188	The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV)	144:393	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	0	28	theme	Physicochemical	0:14	arg1	properties					31:40	Physicochemical and functional properties	0:40	Physicochemical and functional properties of pectin	0:50	Physicochemical and functional properties of pectin extracted from the edible portions of jackfruit at different stages of maturity.					
36534030	9	29	with	pectin	1711:1716	arg1	properties					1735:1744	antioxidant properties	1723:1744	antioxidant properties	1723:1744	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	1	30	theme	properties	501:510	arg1	terms					417:421	terms	417:421	terms of extraction yields, chemical composition, molecular weight, and antioxidant properties	417:510	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	6	31	theme	increased	1354:1362	arg1	content					1364:1370	the increased content	1350:1370	the increased content of galacturonic acid upon fruit development	1350:1414	Although the phenolics and flavonoids content of JFP decreased with fruit maturity, their antioxidant capacity increased, which may be correlated with the increased content of galacturonic acid upon fruit development.					
36534030	4	32	theme	methoxyl	945:952	arg1	type					954:957	the high methoxyl type	936:957	the high methoxyl type	936:957	The JFP was of the high methoxyl type and the degree of esterification increased from 65% to 87% with fruit maturity.					
36534030	1	33	theme	raw	270:272	arg1	jackfruit					274:282	raw jackfruit	270:282	raw jackfruit (an underutilized tropical fruit) at four different maturity stages	270:350	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	1	33	theme	raw	270:272	arg1	fruit					311:315	an underutilized tropical fruit	285:315	an underutilized tropical fruit	285:315	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	0	34	theme	maturity	123:130	arg1	stages					113:118	different stages	103:118	different stages of maturity	103:130	Physicochemical and functional properties of pectin extracted from the edible portions of jackfruit at different stages of maturity.					
36534030	2	35	theme	%	591:591	arg1	%					600:600	9.7% to 21.5%	588:600	9.7% to 21.5% with fruit maturity	588:620	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	9	36	theme	promising	1670:1678	arg1	source					1680:1685	a promising source	1668:1685	a promising source of high-quality gelling pectin with antioxidant properties	1668:1744	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	9	36	theme	promising	1670:1678	arg1	potential					1604:1612	the potential	1600:1612	the potential of pectin extracted from edible parts of jackfruit	1600:1663	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	0	37	theme	functional	20:29	arg1	properties					31:40	Physicochemical and functional properties	0:40	Physicochemical and functional properties of pectin	0:50	Physicochemical and functional properties of pectin extracted from the edible portions of jackfruit at different stages of maturity.					
36534030	2	38	theme	fruit	607:611	arg1	maturity					613:620	fruit maturity	607:620	fruit maturity	607:620	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	1	39	from	use	538:540	arg1	foods					545:549	foods	545:549	foods	545:549	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	7	40	theme	viscoelastic	1447:1458	arg1	behavior					1460:1467	viscoelastic behavior	1447:1467	viscoelastic behavior	1447:1467	Gels prepared from JFP showed viscoelastic behavior.					
36534030	9	41	theme	pectin	1711:1716	arg1	source					1680:1685	a promising source	1668:1685	a promising source of high-quality gelling pectin with antioxidant properties	1668:1744	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	9	41	theme	pectin	1711:1716	arg1	potential					1604:1612	the potential	1600:1612	the potential of pectin extracted from edible parts of jackfruit	1600:1663	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	0	42	theme	pectin	45:50	arg1	properties					31:40	Physicochemical and functional properties	0:40	Physicochemical and functional properties of pectin	0:50	Physicochemical and functional properties of pectin extracted from the edible portions of jackfruit at different stages of maturity.					
36534030	4	43	theme	high	940:943	arg1	type					954:957	the high methoxyl type	936:957	the high methoxyl type	936:957	The JFP was of the high methoxyl type and the degree of esterification increased from 65% to 87% with fruit maturity.					
36534030	2	44	dep	stages	720:725	arg1	I-IV					727:730	I-IV	727:730	stages I-IV respectively	720:743	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	8	45	theme	different	1531:1539	arg1	behavior					1550:1557	different gelation behavior	1531:1557	different gelation behavior	1531:1557	Depending on the maturity stage in which they were obtained, different gelation behavior was seen.					
36534030	6	46	theme	fruit	1267:1271	arg1	maturity					1273:1280	fruit maturity	1267:1280	fruit maturity	1267:1280	Although the phenolics and flavonoids content of JFP decreased with fruit maturity, their antioxidant capacity increased, which may be correlated with the increased content of galacturonic acid upon fruit development.					
36534030	2	47	from	increase	641:648	arg1	content					675:681	the galacturonic acid content	653:681	the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively	653:743	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	4	48	with	%	1016:1016	arg1	maturity					1029:1036	fruit maturity	1023:1036	fruit maturity	1023:1036	The JFP was of the high methoxyl type and the degree of esterification increased from 65% to 87% with fruit maturity.					
36534030	6	49	theme	acid	1388:1391	arg1	content					1364:1370	the increased content	1350:1370	the increased content of galacturonic acid upon fruit development	1350:1414	Although the phenolics and flavonoids content of JFP decreased with fruit maturity, their antioxidant capacity increased, which may be correlated with the increased content of galacturonic acid upon fruit development.					
36534030	3	50	from	147 kDa	782:788	arg1	stage					793:797	stage I to 169 kDa	793:810	stage I to 169 kDa	793:810	The molecular weight increased from 147 kDa in stage I to 169 kDa in stage III, but decreased to 114 kDa in stage IV, probably due to cell-wall degradation during maturation.					
36534030	9	51	theme	antioxidant	1723:1733	arg1	properties					1735:1744	antioxidant properties	1723:1744	antioxidant properties	1723:1744	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	9	52	theme	jackfruit	1655:1663	arg1	parts					1646:1650	edible parts	1639:1650	edible parts of jackfruit	1639:1663	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	1	53	theme	underutilized	288:300	arg1	jackfruit					274:282	raw jackfruit	270:282	raw jackfruit (an underutilized tropical fruit) at four different maturity stages	270:350	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	1	53	theme	underutilized	288:300	arg1	fruit					311:315	an underutilized tropical fruit	285:315	an underutilized tropical fruit	285:315	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	9	54	theme	edible	1639:1644	arg1	parts					1646:1650	edible parts	1639:1650	edible parts of jackfruit	1639:1663	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	1	55	theme	extraction	426:435	arg1	yields					437:442	extraction yields	426:442	extraction yields	426:442	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	1	56	theme	tropical	302:309	arg1	jackfruit					274:282	raw jackfruit	270:282	raw jackfruit (an underutilized tropical fruit) at four different maturity stages	270:350	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	1	56	theme	tropical	302:309	arg1	fruit					311:315	an underutilized tropical fruit	285:315	an underutilized tropical fruit	285:315	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	1	57	dep	seed	261:264	arg1	referred					353:360	referred	353:360	referred to as stages I, II, III, and IV	353:392	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	1	58	theme	yields	437:442	arg1	terms					417:421	terms	417:421	terms of extraction yields, chemical composition, molecular weight, and antioxidant properties	417:510	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	9	59	theme	pectin	1617:1622	arg1	source					1680:1685	a promising source	1668:1685	a promising source of high-quality gelling pectin with antioxidant properties	1668:1744	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	9	59	theme	pectin	1617:1622	arg1	potential					1604:1612	the potential	1600:1612	the potential of pectin extracted from edible parts of jackfruit	1600:1663	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	1	60	theme	potential	528:536	arg1	use					538:540	its potential use	524:540	its potential use in foods	524:549	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	6	61	theme	fruit	1398:1402	arg1	development					1404:1414	fruit development	1398:1414	fruit development	1398:1414	Although the phenolics and flavonoids content of JFP decreased with fruit maturity, their antioxidant capacity increased, which may be correlated with the increased content of galacturonic acid upon fruit development.					
36534030	9	62	dep	CONCLUSION	1569:1578	arg1	confirmed					1590:1598	confirmed	1590:1598	confirmed	1590:1598	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	8	63	theme	gelation	1541:1548	arg1	behavior					1550:1557	different gelation behavior	1531:1557	different gelation behavior	1531:1557	Depending on the maturity stage in which they were obtained, different gelation behavior was seen.					
36534030	10	64	theme	Chemical	1788:1795	arg1	Industry					1797:1804	Chemical Industry	1788:1804	Chemical Industry	1788:1804	© 2022 Society of Chemical Industry.					
36534030	4	65	theme	%	1009:1009	arg1	%					1016:1016	65% to 87%	1007:1016	65% to 87% with fruit maturity	1007:1036	The JFP was of the high methoxyl type and the degree of esterification increased from 65% to 87% with fruit maturity.					
36534030	1	66	theme	edible	221:226	arg1	seed					261:264	seed	261:264	seed	261:264	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	1	66	theme	edible	221:226	arg1	portions					228:235	edible portions	221:235	edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV)	221:393	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	1	66	theme	edible	221:226	arg1	pericarp					248:255	pericarp	248:255	pericarp	248:255	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	1	67	theme	chemical	445:452	arg1	composition					454:464	chemical composition	445:464	chemical composition	445:464	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	2	68	dep	%	600:600	arg1	to					593:594	to	593:594	to	593:594	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	0	69	theme	edible	71:76	arg1	portions					78:85	the edible portions	67:85	the edible portions of jackfruit	67:98	Physicochemical and functional properties of pectin extracted from the edible portions of jackfruit at different stages of maturity.					
36534030	5	70	theme	JFP	1068:1070	arg1	similar					1077:1083	similar	1077:1083	similar	1077:1083	The functional properties of JFP were similar to or better than those reported for commercial apple pectin, thus highlighting its potential as a food additive.					
36534030	5	70	theme	JFP	1068:1070	arg1	properties					1054:1063	The functional properties	1039:1063	The functional properties of JFP	1039:1070	The functional properties of JFP were similar to or better than those reported for commercial apple pectin, thus highlighting its potential as a food additive.					
36534030	1	71	theme	composition	454:464	arg1	terms					417:421	terms	417:421	terms of extraction yields, chemical composition, molecular weight, and antioxidant properties	417:510	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	9	72	theme	gelling	1703:1709	arg1	pectin					1711:1716	high-quality gelling pectin	1690:1716	high-quality gelling pectin with antioxidant properties	1690:1744	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
36534030	4	73	dep	%	1016:1016	arg1	to					1011:1012	to	1011:1012	to	1011:1012	The JFP was of the high methoxyl type and the degree of esterification increased from 65% to 87% with fruit maturity.					
36534030	8	74	theme	maturity	1487:1494	arg1	stage					1496:1500	the maturity stage	1483:1500	the maturity stage in which they were obtained	1483:1528	Depending on the maturity stage in which they were obtained, different gelation behavior was seen.					
36534030	5	75	theme	commercial	1122:1131	arg1	pectin					1139:1144	commercial apple pectin	1122:1144	commercial apple pectin	1122:1144	The functional properties of JFP were similar to or better than those reported for commercial apple pectin, thus highlighting its potential as a food additive.					
36534030	1	76	from	stages	345:350	arg1	jackfruit					274:282	raw jackfruit	270:282	raw jackfruit (an underutilized tropical fruit) at four different maturity stages	270:350	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	1	76	from	stages	345:350	arg1	fruit					311:315	an underutilized tropical fruit	285:315	an underutilized tropical fruit	285:315	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	4	77	theme	esterification	977:990	arg1	degree					967:972	the degree	963:972	the degree of esterification	963:990	The JFP was of the high methoxyl type and the degree of esterification increased from 65% to 87% with fruit maturity.					
36534030	1	78	theme	physicochemical	148:162	arg1	properties					179:188	The physicochemical and functional properties	144:188	The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV)	144:393	BACKGROUND The physicochemical and functional properties of pectin (JFP) extracted from edible portions (including pericarp and seed) of raw jackfruit (an underutilized tropical fruit) at four different maturity stages (referred to as stages I, II, III, and IV) were characterized in terms of extraction yields, chemical composition, molecular weight, and antioxidant properties to evaluate its potential use in foods.					
36534030	2	79	dep	increased	573:581	arg1	accompanied					623:633	accompanied	623:633	accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively	623:743	RESULT The JFP yield increased from 9.7% to 21.5% with fruit maturity, accompanied by an increase in the galacturonic acid content (50.1%, 57.1%, 63.6%, and 65.2%) for stages I-IV respectively.					
36534030	9	80	theme	food	1751:1754	arg1	applications					1756:1767	food applications	1751:1767	food applications	1751:1767	CONCLUSION The study confirmed the potential of pectin extracted from edible parts of jackfruit as a promising source of high-quality gelling pectin with antioxidant properties, for food applications.					
35577841	0	0	theme	sodium	75:80	arg1	hyaluronate					82:92	sodium hyaluronate	75:92	sodium hyaluronate	75:92	New approach to prepare cytocompatible 3D scaffolds via the combination of sodium hyaluronate and colloidal particles of conductive polymers.					
35577841	2	1	with	crosslinking	388:399	arg1	N-					406:407	N-	406:407	N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid	406:487	For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) was used.					
35577841	5	2	contain	containing	925:934	arg2	content					948:954	the highest content	936:954	the highest content of polypyrrole particles	936:979	The elasticity modulus depended on the composition of the scaffolds, with the highest value of ~ 50 kPa obtained for the sample containing the highest content of polypyrrole particles.					
35577841	5	2	contain	containing	925:934	arg1	sample					918:923	the sample	914:923	the sample containing the highest content of polypyrrole particles	914:979	The elasticity modulus depended on the composition of the scaffolds, with the highest value of ~ 50 kPa obtained for the sample containing the highest content of polypyrrole particles.					
35577841	5	3	theme	highest	940:946	arg1	content					948:954	the highest content	936:954	the highest content of polypyrrole particles	936:979	The elasticity modulus depended on the composition of the scaffolds, with the highest value of ~ 50 kPa obtained for the sample containing the highest content of polypyrrole particles.					
35577841	8	4	with	solubility	1313:1322	arg1	matrices					1406:1413	other hydrophilic polymer matrices	1380:1413	other hydrophilic polymer matrices	1380:1413	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	1	5	theme	particles	286:294	arg1	dispersion					230:239	a colloidal dispersion	218:239	a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w)	218:342	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35577841	2	6	theme	poly	536:539	arg1	presence					524:531	the presence	520:531	the presence of poly(vinylalcohol)	520:553	For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) was used.					
35577841	2	7	from	process	509:515	arg1	presence					524:531	the presence	520:531	the presence of poly(vinylalcohol)	520:553	For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) was used.					
35577841	8	8	theme	limited	1355:1361	arg1	miscibility					1363:1373	limited miscibility	1355:1373	limited miscibility with other hydrophilic polymer matrices	1355:1413	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	8	9	theme	cell-instructive	1499:1514	arg1	properties					1516:1525	potentially cell-instructive properties	1487:1525	potentially cell-instructive properties	1487:1525	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	3	10	theme	interconnected	589:602	arg1	pores					604:608	interconnected pores	589:608	interconnected pores with prevailing porosity values of ~ 30%	589:649	The scaffolds comprised interconnected pores with prevailing porosity values of ~ 30% and pore sizes enabling the accommodation of cells.					
35577841	0	11	theme	hyaluronate	82:92	arg1	combination					60:70	the combination	56:70	the combination of sodium hyaluronate and colloidal particles of conductive polymers	56:139	New approach to prepare cytocompatible 3D scaffolds via the combination of sodium hyaluronate and colloidal particles of conductive polymers.					
35577841	8	12	theme	damaged	1572:1578	arg1	tissues					1598:1604	damaged electro-sensitive tissues	1572:1604	damaged electro-sensitive tissues	1572:1604	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	6	13	theme	cell	1037:1040	arg1	adhesion					1042:1049	cell adhesion	1037:1049	cell adhesion	1037:1049	The scaffolds did not possess cytotoxicity and allowed cell adhesion and growth on the surface.					
35577841	0	14	theme	particles	108:116	arg1	combination					60:70	the combination	56:70	the combination of sodium hyaluronate and colloidal particles of conductive polymers	56:139	New approach to prepare cytocompatible 3D scaffolds via the combination of sodium hyaluronate and colloidal particles of conductive polymers.					
35577841	5	15	theme	highest	875:881	arg1	of ~ 50 kPa					889:899	the highest value of ~ 50 kPa	871:899	the highest value of ~ 50 kPa obtained for the sample containing the highest content of polypyrrole particles	871:979	The elasticity modulus depended on the composition of the scaffolds, with the highest value of ~ 50 kPa obtained for the sample containing the highest content of polypyrrole particles.					
35577841	8	16	theme	polymer	1398:1404	arg1	matrices					1406:1413	other hydrophilic polymer matrices	1380:1413	other hydrophilic polymer matrices	1380:1413	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	0	17	theme	colloidal	98:106	arg1	particles					108:116	colloidal particles	98:116	colloidal particles of conductive polymers	98:139	New approach to prepare cytocompatible 3D scaffolds via the combination of sodium hyaluronate and colloidal particles of conductive polymers.					
35577841	8	18	theme	tissues	1598:1604	arg1	healing					1561:1567	the healing	1557:1567	the healing of damaged electro-sensitive tissues	1557:1604	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	7	19	from	conditions	1106:1115	arg1	bioreactor					1122:1131	a bioreactor	1120:1131	a bioreactor	1120:1131	Using the in vivo-mimicking conditions in a bioreactor, cells were also able to grow into the structure of the scaffolds.					
35577841	8	20	from	miscibility	1363:1373	arg1	environment					1338:1348	an aqueous environment	1327:1348	an aqueous environment	1327:1348	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	2	21	theme	freeze-thawing	494:507	arg1	process					509:515	a freeze-thawing process	492:515	a freeze-thawing process in the presence of poly(vinylalcohol)	492:553	For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) was used.					
35577841	1	22	theme	sodium	188:193	arg1	hyaluronate					195:205	sodium hyaluronate	188:205	sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w)	188:342	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35577841	8	23	theme	scaffold	1217:1224	arg1	preparation					1226:1236	scaffold preparation	1217:1236	scaffold preparation	1217:1236	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	8	24	theme	electro-sensitive	1580:1596	arg1	tissues					1598:1604	damaged electro-sensitive tissues	1572:1604	damaged electro-sensitive tissues	1572:1604	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	3	25	theme	porosity	626:633	arg1	values					635:640	prevailing porosity values	615:640	prevailing porosity values	615:640	The scaffolds comprised interconnected pores with prevailing porosity values of ~ 30% and pore sizes enabling the accommodation of cells.					
35577841	8	26	theme	polymers	1293:1300	arg1	limitations					1267:1277	the limitations	1263:1277	the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices)	1263:1414	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	0	27	theme	New	0:2	arg1	approach					4:11	New approach	0:11	New approach	0:11	New approach to prepare cytocompatible 3D scaffolds via the combination of sodium hyaluronate and colloidal particles of conductive polymers.					
35577841	8	28	theme	conductive	1282:1291	arg1	polymers					1293:1300	conductive polymers	1282:1300	conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices)	1282:1414	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	7	29	theme	scaffolds	1189:1197	arg1	structure					1172:1180	the structure	1168:1180	the structure of the scaffolds	1168:1197	Using the in vivo-mimicking conditions in a bioreactor, cells were also able to grow into the structure of the scaffolds.					
35577841	5	30	theme	scaffolds	855:863	arg1	composition					836:846	the composition	832:846	the composition of the scaffolds	832:863	The elasticity modulus depended on the composition of the scaffolds, with the highest value of ~ 50 kPa obtained for the sample containing the highest content of polypyrrole particles.					
35577841	0	31	theme	conductive	121:130	arg1	polymers					132:139	conductive polymers	121:139	conductive polymers	121:139	New approach to prepare cytocompatible 3D scaffolds via the combination of sodium hyaluronate and colloidal particles of conductive polymers.					
35577841	8	32	dep	solubility	1313:1322	arg1	e.g.					1303:1306	e.g.	1303:1306	e.g.	1303:1306	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	8	33	theme	preparation	1226:1236	arg1	technique					1204:1212	The technique	1200:1212	The technique of scaffold preparation used here	1200:1246	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	1	34	contain	containing	207:216	arg1	hyaluronate					195:205	sodium hyaluronate	188:205	sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w)	188:342	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35577841	1	34	contain	containing	207:216	arg2	dispersion					230:239	a colloidal dispersion	218:239	a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w)	218:342	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35577841	3	35	theme	prevailing	615:624	arg1	values					635:640	prevailing porosity values	615:640	prevailing porosity values	615:640	The scaffolds comprised interconnected pores with prevailing porosity values of ~ 30% and pore sizes enabling the accommodation of cells.					
35577841	1	36	theme	colloidal	220:228	arg1	dispersion					230:239	a colloidal dispersion	218:239	a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w)	218:342	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35577841	0	37	theme	3D	39:40	arg1	scaffolds					42:50	cytocompatible 3D scaffolds	24:50	cytocompatible 3D scaffolds	24:50	New approach to prepare cytocompatible 3D scaffolds via the combination of sodium hyaluronate and colloidal particles of conductive polymers.					
35577841	8	38	with	scaffolds	1472:1480	arg1	properties					1516:1525	potentially cell-instructive properties	1487:1525	potentially cell-instructive properties	1487:1525	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	2	39	used	used	559:562	arg2	crosslinking					388:399	crosslinking	388:399	crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid	388:487	For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) was used.					
35577841	2	39	used	used	559:562	arg2	process					509:515	a freeze-thawing process	492:515	a freeze-thawing process in the presence of poly(vinylalcohol)	492:553	For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) was used.					
35577841	6	40	contain	possess	1004:1010	arg2	cytotoxicity					1012:1023	cytotoxicity	1012:1023	cytotoxicity	1012:1023	The scaffolds did not possess cytotoxicity and allowed cell adhesion and growth on the surface.					
35577841	6	40	contain	possess	1004:1010	arg1	scaffolds					986:994	The scaffolds	982:994	The scaffolds	982:994	The scaffolds did not possess cytotoxicity and allowed cell adhesion and growth on the surface.					
35577841	5	41	theme	value	883:887	arg1	of ~ 50 kPa					889:899	the highest value of ~ 50 kPa	871:899	the highest value of ~ 50 kPa obtained for the sample containing the highest content of polypyrrole particles	871:979	The elasticity modulus depended on the composition of the scaffolds, with the highest value of ~ 50 kPa obtained for the sample containing the highest content of polypyrrole particles.					
35577841	0	42	theme	cytocompatible	24:37	arg1	scaffolds					42:50	cytocompatible 3D scaffolds	24:50	cytocompatible 3D scaffolds	24:50	New approach to prepare cytocompatible 3D scaffolds via the combination of sodium hyaluronate and colloidal particles of conductive polymers.					
35577841	0	43	theme	polymers	132:139	arg1	hyaluronate					82:92	sodium hyaluronate	75:92	sodium hyaluronate	75:92	New approach to prepare cytocompatible 3D scaffolds via the combination of sodium hyaluronate and colloidal particles of conductive polymers.					
35577841	0	43	theme	polymers	132:139	arg1	particles					108:116	colloidal particles	98:116	colloidal particles of conductive polymers	98:139	New approach to prepare cytocompatible 3D scaffolds via the combination of sodium hyaluronate and colloidal particles of conductive polymers.					
35577841	3	44	theme	pore	655:658	arg1	sizes					660:664	pore sizes	655:664	pore sizes enabling the accommodation of cells	655:700	The scaffolds comprised interconnected pores with prevailing porosity values of ~ 30% and pore sizes enabling the accommodation of cells.					
35577841	8	45	theme	aqueous	1330:1336	arg1	environment					1338:1348	an aqueous environment	1327:1348	an aqueous environment	1327:1348	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	2	46	theme	3-dimethylaminopropyl-N-ethylcarbodiimide	409:449	arg1	hydrochloride					451:463	3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride	409:463	3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride	409:463	For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) was used.					
35577841	1	47	theme	%	337:337	arg1	concentrations					297:310	concentrations	297:310	concentrations of 0.108, 0.054 and 0.036% w/w	297:341	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35577841	3	48	theme	cells	696:700	arg1	accommodation					679:691	the accommodation	675:691	the accommodation of cells	675:700	The scaffolds comprised interconnected pores with prevailing porosity values of ~ 30% and pore sizes enabling the accommodation of cells.					
35577841	3	49	theme	of ~ 30	642:648	arg1	%					649:649	%	649:649	%	649:649	The scaffolds comprised interconnected pores with prevailing porosity values of ~ 30% and pore sizes enabling the accommodation of cells.					
35577841	2	50	from	crosslinking	388:399	arg1	presence					524:531	the presence	520:531	the presence of poly(vinylalcohol)	520:553	For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) was used.					
35577841	4	51	theme	disintegration	753:766	arg1	sign					745:748	any sign	741:748	any sign of disintegration	741:766	A swelling capacity of 92-97% without any sign of disintegration was typical for all samples.					
35577841	3	52	with	pores	604:608	arg1	values					635:640	prevailing porosity values	615:640	prevailing porosity values	615:640	The scaffolds comprised interconnected pores with prevailing porosity values of ~ 30% and pore sizes enabling the accommodation of cells.					
35577841	1	53	theme	w/w	339:341	arg1	%					337:337	0.108, 0.054 and 0.036% w/w	315:341	0.108, 0.054 and 0.036% w/w	315:341	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35577841	7	54	theme	vivo-mimicking	1091:1104	arg1	conditions					1106:1115	the in vivo-mimicking conditions	1084:1115	the in vivo-mimicking conditions in a bioreactor	1084:1131	Using the in vivo-mimicking conditions in a bioreactor, cells were also able to grow into the structure of the scaffolds.					
35577841	8	55	with	miscibility	1363:1373	arg1	matrices					1406:1413	other hydrophilic polymer matrices	1380:1413	other hydrophilic polymer matrices	1380:1413	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	3	56	theme	%	649:649	arg1	pores					604:608	interconnected pores	589:608	interconnected pores with prevailing porosity values of ~ 30%	589:649	The scaffolds comprised interconnected pores with prevailing porosity values of ~ 30% and pore sizes enabling the accommodation of cells.					
35577841	2	57	with	process	509:515	arg1	N-					406:407	N-	406:407	N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid	406:487	For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) was used.					
35577841	5	58	theme	polypyrrole	959:969	arg1	particles					971:979	polypyrrole particles	959:979	polypyrrole particles	959:979	The elasticity modulus depended on the composition of the scaffolds, with the highest value of ~ 50 kPa obtained for the sample containing the highest content of polypyrrole particles.					
35577841	8	59	theme	scaffolds	1472:1480	arg1	preparation					1442:1452	the preparation	1438:1452	the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues	1438:1604	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	8	60	theme	cytocompatible	1457:1470	arg1	scaffolds					1472:1480	cytocompatible scaffolds	1457:1480	cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues	1457:1604	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	5	61	theme	particles	971:979	arg1	content					948:954	the highest content	936:954	the highest content of polypyrrole particles	936:979	The elasticity modulus depended on the composition of the scaffolds, with the highest value of ~ 50 kPa obtained for the sample containing the highest content of polypyrrole particles.					
35577841	8	62	from	advantage	1544:1552	arg1	healing					1561:1567	the healing	1557:1567	the healing of damaged electro-sensitive tissues	1557:1604	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	1	63	theme	water-miscible	244:257	arg1	polyaniline					259:269	water-miscible polyaniline	244:269	water-miscible polyaniline	244:269	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35577841	8	64	theme	hydrophilic	1386:1396	arg1	matrices					1406:1413	other hydrophilic polymer matrices	1380:1413	other hydrophilic polymer matrices	1380:1413	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	1	65	dep	particles	286:294	arg1	concentrations					297:310	concentrations	297:310	concentrations of 0.108, 0.054 and 0.036% w/w	297:341	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35577841	8	66	theme	other	1380:1384	arg1	matrices					1406:1413	other hydrophilic polymer matrices	1380:1413	other hydrophilic polymer matrices	1380:1413	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	8	67	from	solubility	1313:1322	arg1	environment					1338:1348	an aqueous environment	1327:1348	an aqueous environment	1327:1348	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	4	68	theme	%	731:731	arg1	capacity					714:721	A swelling capacity	703:721	A swelling capacity of 92-97% without any sign of disintegration	703:766	A swelling capacity of 92-97% without any sign of disintegration was typical for all samples.					
35577841	4	68	theme	%	731:731	arg1	typical					772:778	typical	772:778	typical	772:778	A swelling capacity of 92-97% without any sign of disintegration was typical for all samples.					
35577841	7	69	dep	vivo-mimicking	1091:1104	arg1	in					1088:1089	in	1088:1089	in	1088:1089	Using the in vivo-mimicking conditions in a bioreactor, cells were also able to grow into the structure of the scaffolds.					
35577841	1	70	theme	polyaniline	259:269	arg1	dispersion					230:239	a colloidal dispersion	218:239	a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w)	218:342	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35577841	3	71	with	sizes	660:664	arg1	values					635:640	prevailing porosity values	615:640	prevailing porosity values	615:640	The scaffolds comprised interconnected pores with prevailing porosity values of ~ 30% and pore sizes enabling the accommodation of cells.					
35577841	8	72	theme	poor	1308:1311	arg1	solubility					1313:1322	poor solubility	1308:1322	poor solubility in an aqueous environment	1308:1348	The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.					
35577841	1	73	theme	Bio-inspired	142:153	arg1	scaffolds					166:174	Bio-inspired conductive scaffolds	142:174	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w)	142:342	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35577841	4	74	theme	swelling	705:712	arg1	capacity					714:721	A swelling capacity	703:721	A swelling capacity of 92-97% without any sign of disintegration	703:766	A swelling capacity of 92-97% without any sign of disintegration was typical for all samples.					
35577841	4	74	theme	swelling	705:712	arg1	typical					772:778	typical	772:778	typical	772:778	A swelling capacity of 92-97% without any sign of disintegration was typical for all samples.					
35577841	5	75	theme	elasticity	801:810	arg1	modulus					812:818	The elasticity modulus	797:818	The elasticity modulus	797:818	The elasticity modulus depended on the composition of the scaffolds, with the highest value of ~ 50 kPa obtained for the sample containing the highest content of polypyrrole particles.					
35577841	2	76	dep	N-	406:407	arg1	hydrochloride					451:463	3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride	409:463	3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride	409:463	For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) was used.					
35577841	2	76	dep	N-	406:407	arg1	N-hydroxysuccinimid					469:487	N-hydroxysuccinimid	469:487	N-hydroxysuccinimid	469:487	For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) was used.					
35577841	1	77	theme	conductive	155:164	arg1	scaffolds					166:174	Bio-inspired conductive scaffolds	142:174	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w)	142:342	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35577841	1	78	theme	polypyrrole	274:284	arg1	particles					286:294	polypyrrole particles	274:294	polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w)	274:342	Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured.					
35834652	3	0	theme	complex	667:673	arg1	composition					611:621	the composition	607:621	the composition of the phosphatidylinositol 4-kinase enzyme complex	607:673	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	0	1	theme	Increased	62:70	arg1	Production					72:81	Increased Production	62:81	Increased Production of Multiple Cytokines	62:103	Knockdown of Transmembrane Protein 150A (TMEM150A) Results in Increased Production of Multiple Cytokines.					
35834652	3	2	theme	phosphatidylinositol	630:649	arg1	4-kinase					651:658	phosphatidylinositol 4-kinase	630:658	the phosphatidylinositol 4-kinase enzyme complex	626:673	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	6	3	from	knockdown	994:1002	arg1	H292					1048:1051	H292	1048:1051	H292	1048:1051	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	6	3	from	knockdown	994:1002	arg1	line					1042:1045	lung epithelial cell line	1021:1045	a lung epithelial cell line (H292)	1019:1052	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	6	4	theme	increased	1066:1074	arg1	levels					1085:1090	increased cytokine levels	1066:1090	increased cytokine levels in the unstimulated conditions	1066:1121	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	1	5	theme	Toll-like	157:165	arg1	receptor					167:174	Toll-like receptor 4	157:176	Toll-like receptor 4 (TLR4)	157:183	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	1	5	theme	Toll-like	157:165	arg1	TLR4					179:182	TLR4	179:182	TLR4	179:182	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	0	6	theme	Multiple	86:93	arg1	Cytokines					95:103	Multiple Cytokines	86:103	Multiple Cytokines	86:103	Knockdown of Transmembrane Protein 150A (TMEM150A) Results in Increased Production of Multiple Cytokines.					
35834652	3	7	theme	4-kinase	651:658	arg1	complex					667:673	the phosphatidylinositol 4-kinase enzyme complex	626:673	the phosphatidylinositol 4-kinase enzyme complex	626:673	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	3	8	theme	TMEM150A/TM6P1/damage-regulated	468:498	arg1	modulator					510:518	TMEM150A/TM6P1/damage-regulated autophagy modulator 5	468:520	TMEM150A/TM6P1/damage-regulated autophagy modulator 5	468:520	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	3	8	theme	TMEM150A/TM6P1/damage-regulated	468:498	arg1	150A					462:465	transmembrane protein 150A	440:465	transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5)	440:521	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	5	9	theme	secretion	947:955	arg1	levels					916:921	increased levels	906:921	increased levels of LPS-induced cytokine secretion and transcript levels	906:977	In general, decreased expression of TMEM150A led to increased levels of LPS-induced cytokine secretion and transcript levels.					
35834652	7	10	theme	Future	1192:1197	arg1	studies					1199:1205	Future studies	1192:1205	Future studies	1192:1205	Future studies will investigate if TMEM150A plays a similar role for other TLR agonists and in other cell lineages.					
35834652	6	11	from	levels	1085:1090	arg1	conditions					1112:1121	the unstimulated conditions	1095:1121	the unstimulated conditions	1095:1121	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	1	12	theme	inositol	318:325	arg1	derivatives					287:297	its derivatives	283:297	its derivatives diacylglycerol and inositol trisphosphate	283:339	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	1	12	theme	inositol	318:325	arg1	trisphosphate					327:339	inositol trisphosphate	318:339	inositol trisphosphate	318:339	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	6	13	theme	cell	1037:1040	arg1	H292					1048:1051	H292	1048:1051	H292	1048:1051	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	6	13	theme	cell	1037:1040	arg1	line					1042:1045	lung epithelial cell line	1021:1045	a lung epithelial cell line (H292)	1019:1052	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	5	14	theme	transcript	961:970	arg1	levels					972:977	transcript levels	961:977	transcript levels	961:977	In general, decreased expression of TMEM150A led to increased levels of LPS-induced cytokine secretion and transcript levels.					
35834652	0	15	theme	Cytokines	95:103	arg1	Production					72:81	Increased Production	62:81	Increased Production of Multiple Cytokines	62:103	Knockdown of Transmembrane Protein 150A (TMEM150A) Results in Increased Production of Multiple Cytokines.					
35834652	4	16	theme	TLR4-expressing	763:777	arg1	cells					790:794	TLR4-expressing epithelial cells	763:794	TLR4-expressing epithelial cells	763:794	To determine if TMEM150A function impacts TLR4 signaling, TMEM150A was knocked down in TLR4-expressing epithelial cells and cytokine expression quantified after LPS stimulation.					
35834652	7	17	theme	other	1287:1291	arg1	lineages					1298:1305	other cell lineages	1287:1305	other cell lineages	1287:1305	Future studies will investigate if TMEM150A plays a similar role for other TLR agonists and in other cell lineages.					
35834652	5	18	theme	levels	972:977	arg1	levels					916:921	increased levels	906:921	increased levels of LPS-induced cytokine secretion and transcript levels	906:977	In general, decreased expression of TMEM150A led to increased levels of LPS-induced cytokine secretion and transcript levels.					
35834652	2	19	theme	PI	352:353	arg1	P2					359:360	PI(4,5)P2	352:360	PI(4,5)P2	352:360	Levels of PI(4,5)P2 are controlled enzymatically and fluctuate in LPS-stimulated cells.					
35834652	4	20	theme	LPS	837:839	arg1	stimulation					841:851	LPS stimulation	837:851	LPS stimulation	837:851	To determine if TMEM150A function impacts TLR4 signaling, TMEM150A was knocked down in TLR4-expressing epithelial cells and cytokine expression quantified after LPS stimulation.					
35834652	7	21	theme	cell	1293:1296	arg1	lineages					1298:1305	other cell lineages	1287:1305	other cell lineages	1287:1305	Future studies will investigate if TMEM150A plays a similar role for other TLR agonists and in other cell lineages.					
35834652	1	22	dep	derivatives	287:297	arg1	derivatives					287:297	its derivatives	283:297	its derivatives diacylglycerol and inositol trisphosphate	283:339	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	1	22	dep	derivatives	287:297	arg1	diacylglycerol					299:312	diacylglycerol	299:312	diacylglycerol	299:312	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	1	22	dep	derivatives	287:297	arg1	trisphosphate					327:339	inositol trisphosphate	318:339	inositol trisphosphate	318:339	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	4	23	theme	TMEM150A	692:699	arg1	function					701:708	TMEM150A function	692:708	TMEM150A function	692:708	To determine if TMEM150A function impacts TLR4 signaling, TMEM150A was knocked down in TLR4-expressing epithelial cells and cytokine expression quantified after LPS stimulation.					
35834652	0	24	theme	Protein	27:33	arg1	TMEM150A					41:48	TMEM150A	41:48	TMEM150A	41:48	Knockdown of Transmembrane Protein 150A (TMEM150A) Results in Increased Production of Multiple Cytokines.					
35834652	0	24	theme	Protein	27:33	arg1	150A					35:38	Transmembrane Protein 150A	13:38	Transmembrane Protein 150A (TMEM150A)	13:49	Knockdown of Transmembrane Protein 150A (TMEM150A) Results in Increased Production of Multiple Cytokines.					
35834652	1	25	theme	-bisphosphate	253:265	arg1	P2					275:276	phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2	227:276	phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2	227:276	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	4	26	theme	cytokine	800:807	arg1	expression					809:818	cytokine expression	800:818	cytokine expression	800:818	To determine if TMEM150A function impacts TLR4 signaling, TMEM150A was knocked down in TLR4-expressing epithelial cells and cytokine expression quantified after LPS stimulation.					
35834652	0	27	theme	Transmembrane	13:25	arg1	TMEM150A					41:48	TMEM150A	41:48	TMEM150A	41:48	Knockdown of Transmembrane Protein 150A (TMEM150A) Results in Increased Production of Multiple Cytokines.					
35834652	0	27	theme	Transmembrane	13:25	arg1	150A					35:38	Transmembrane Protein 150A	13:38	Transmembrane Protein 150A (TMEM150A)	13:49	Knockdown of Transmembrane Protein 150A (TMEM150A) Results in Increased Production of Multiple Cytokines.					
35834652	1	28	theme	[PI	267:269	arg1	P2					275:276	phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2	227:276	phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2	227:276	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	6	29	theme	TMEM150A	1007:1014	arg1	knockdown					994:1002	knockdown	994:1002	knockdown of TMEM150A in a lung epithelial cell line (H292)	994:1052	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	5	30	theme	cytokine	938:945	arg1	secretion					947:955	LPS-induced cytokine secretion	926:955	LPS-induced cytokine secretion	926:955	In general, decreased expression of TMEM150A led to increased levels of LPS-induced cytokine secretion and transcript levels.					
35834652	2	31	theme	P2	359:360	arg1	Levels					342:347	Levels	342:347	Levels of PI(4,5)P2	342:360	Levels of PI(4,5)P2 are controlled enzymatically and fluctuate in LPS-stimulated cells.					
35834652	7	32	theme	TLR	1267:1269	arg1	agonists					1271:1278	other TLR agonists	1261:1278	other TLR agonists	1261:1278	Future studies will investigate if TMEM150A plays a similar role for other TLR agonists and in other cell lineages.					
35834652	1	33	dep	lipid	220:224	arg1	P2					275:276	phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2	227:276	phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2	227:276	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	3	34	theme	4,5	553:555	arg1	P2					557:558	PI(4,5)P2	550:558	PI(4,5)P2 production	550:569	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	6	35	theme	epithelial	1026:1035	arg1	H292					1048:1051	H292	1048:1051	H292	1048:1051	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	6	35	theme	epithelial	1026:1035	arg1	line					1042:1045	lung epithelial cell line	1021:1045	a lung epithelial cell line (H292)	1019:1052	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	0	36	theme	150A	35:38	arg1	Knockdown					0:8	Knockdown	0:8	Knockdown of Transmembrane Protein 150A (TMEM150A)	0:49	Knockdown of Transmembrane Protein 150A (TMEM150A) Results in Increased Production of Multiple Cytokines.					
35834652	1	37	theme	4,5	271:273	arg1	P2					275:276	phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2	227:276	phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2	227:276	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	3	38	theme	plasma	578:583	arg1	membrane					585:592	the plasma membrane	574:592	the plasma membrane	574:592	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	4	39	theme	epithelial	779:788	arg1	cells					790:794	TLR4-expressing epithelial cells	763:794	TLR4-expressing epithelial cells	763:794	To determine if TMEM150A function impacts TLR4 signaling, TMEM150A was knocked down in TLR4-expressing epithelial cells and cytokine expression quantified after LPS stimulation.					
35834652	2	40	theme	4,5	355:357	arg1	P2					359:360	PI(4,5)P2	352:360	PI(4,5)P2	352:360	Levels of PI(4,5)P2 are controlled enzymatically and fluctuate in LPS-stimulated cells.					
35834652	2	41	theme	LPS-stimulated	408:421	arg1	cells					423:427	LPS-stimulated cells	408:427	LPS-stimulated cells	408:427	Levels of PI(4,5)P2 are controlled enzymatically and fluctuate in LPS-stimulated cells.					
35834652	6	42	theme	lung	1021:1024	arg1	H292					1048:1051	H292	1048:1051	H292	1048:1051	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	6	42	theme	lung	1021:1024	arg1	line					1042:1045	lung epithelial cell line	1021:1045	a lung epithelial cell line (H292)	1019:1052	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	3	43	theme	PI	550:551	arg1	P2					557:558	PI(4,5)P2	550:558	PI(4,5)P2 production	550:569	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	6	44	theme	cytokine	1076:1083	arg1	levels					1085:1090	increased cytokine levels	1066:1090	increased cytokine levels in the unstimulated conditions	1066:1121	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	3	45	theme	transmembrane	440:452	arg1	modulator					510:518	TMEM150A/TM6P1/damage-regulated autophagy modulator 5	468:520	TMEM150A/TM6P1/damage-regulated autophagy modulator 5	468:520	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	3	45	theme	transmembrane	440:452	arg1	150A					462:465	transmembrane protein 150A	440:465	transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5)	440:521	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	5	46	theme	LPS-induced	926:936	arg1	secretion					947:955	LPS-induced cytokine secretion	926:955	LPS-induced cytokine secretion	926:955	In general, decreased expression of TMEM150A led to increased levels of LPS-induced cytokine secretion and transcript levels.					
35834652	7	47	theme	other	1261:1265	arg1	agonists					1271:1278	other TLR agonists	1261:1278	other TLR agonists	1261:1278	Future studies will investigate if TMEM150A plays a similar role for other TLR agonists and in other cell lineages.					
35834652	3	48	theme	protein	454:460	arg1	modulator					510:518	TMEM150A/TM6P1/damage-regulated autophagy modulator 5	468:520	TMEM150A/TM6P1/damage-regulated autophagy modulator 5	468:520	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	3	48	theme	protein	454:460	arg1	150A					462:465	transmembrane protein 150A	440:465	transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5)	440:521	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	7	49	theme	similar	1244:1250	arg1	role					1252:1255	a similar role	1242:1255	a similar role for other TLR agonists and in other cell lineages	1242:1305	Future studies will investigate if TMEM150A plays a similar role for other TLR agonists and in other cell lineages.					
35834652	7	50	from	role	1252:1255	arg1	lineages					1298:1305	other cell lineages	1287:1305	other cell lineages	1287:1305	Future studies will investigate if TMEM150A plays a similar role for other TLR agonists and in other cell lineages.					
35834652	4	51	theme	TLR4	718:721	arg1	signaling					723:731	TLR4 signaling	718:731	TLR4 signaling	718:731	To determine if TMEM150A function impacts TLR4 signaling, TMEM150A was knocked down in TLR4-expressing epithelial cells and cytokine expression quantified after LPS stimulation.					
35834652	5	52	theme	TMEM150A	890:897	arg1	expression					876:885	decreased expression	866:885	decreased expression of TMEM150A	866:897	In general, decreased expression of TMEM150A led to increased levels of LPS-induced cytokine secretion and transcript levels.					
35834652	5	53	theme	increased	906:914	arg1	levels					916:921	increased levels	906:921	increased levels of LPS-induced cytokine secretion and transcript levels	906:977	In general, decreased expression of TMEM150A led to increased levels of LPS-induced cytokine secretion and transcript levels.					
35834652	6	54	theme	unstimulated	1099:1110	arg1	conditions					1112:1121	the unstimulated conditions	1095:1121	the unstimulated conditions	1095:1121	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	1	55	theme	plasma	204:209	arg1	lipid					220:224	the plasma membrane lipid	200:224	the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2]	200:277	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	5	56	theme	decreased	866:874	arg1	expression					876:885	decreased expression	866:885	decreased expression of TMEM150A	866:897	In general, decreased expression of TMEM150A led to increased levels of LPS-induced cytokine secretion and transcript levels.					
35834652	3	57	theme	P2	557:558	arg1	production					560:569	PI(4,5)P2 production	550:569	PI(4,5)P2 production	550:569	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	1	58	theme	-induced	130:137	arg1	signaling					139:147	Lipopolysaccharide (LPS)-induced signaling	106:147	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4)	106:183	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	6	59	theme	important	1152:1160	arg1	role					1162:1165	an important role	1149:1165	an important role	1149:1165	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	1	60	theme	membrane	211:218	arg1	lipid					220:224	the plasma membrane lipid	200:224	the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2]	200:277	Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and its derivatives diacylglycerol and inositol trisphosphate.					
35834652	3	61	theme	enzyme	660:665	arg1	complex					667:673	the phosphatidylinositol 4-kinase enzyme complex	626:673	the phosphatidylinositol 4-kinase enzyme complex	626:673	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	6	62	theme	cellular	1170:1177	arg1	homeostasis					1179:1189	cellular homeostasis	1170:1189	cellular homeostasis	1170:1189	Unexpectedly, knockdown of TMEM150A in a lung epithelial cell line (H292) also led to increased cytokine levels in the unstimulated conditions suggesting TMEM150A plays an important role in cellular homeostasis.					
35834652	3	63	theme	autophagy	500:508	arg1	modulator					510:518	TMEM150A/TM6P1/damage-regulated autophagy modulator 5	468:520	TMEM150A/TM6P1/damage-regulated autophagy modulator 5	468:520	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
35834652	3	63	theme	autophagy	500:508	arg1	150A					462:465	transmembrane protein 150A	440:465	transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5)	440:521	Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P2 production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex.					
36997047	8	0	theme	glucose	935:941	arg1	tolerance					943:951	glucose tolerance	935:951	glucose tolerance	935:951	H2 improved the hepatic histological changes and glucose tolerance, decreased the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase, and relieved liver inflammation.					
36997047	11	1	theme	zonula	1583:1588	arg1	occludens-1					1590:1600	zonula occludens-1	1583:1600	zonula occludens-1	1583:1600	H2 also improved the intestinal tight junction barrier by enhancing the expressions of zonula occludens-1 and occluding.					
36997047	11	2	theme	tight	1528:1532	arg1	barrier					1543:1549	the intestinal tight junction barrier	1513:1549	the intestinal tight junction barrier	1513:1549	H2 also improved the intestinal tight junction barrier by enhancing the expressions of zonula occludens-1 and occluding.					
36997047	13	3	theme	LPS/TLR4/NF-κB	1937:1950	arg1	pathway					1965:1971	LPS/TLR4/NF-κB inflammatory pathway	1937:1971	LPS/TLR4/NF-κB inflammatory pathway	1937:1971	Collectively, our data show that H2 could prevent NAFLD induced by high-fat diet, and the anti-NAFLD effect is associated with the modulation of gut microbiota and inhibition of LPS/TLR4/NF-κB inflammatory pathway.					
36997047	7	4	theme	contents	805:812	arg1	16 S-seq					787:794	16 S-seq	787:794	16 S-seq	787:794	Transcriptome sequencing of liver and 16 S-seq of cecal contents were also performed to explore the related mechanisms of H2 inhalation.					
36997047	7	4	theme	contents	805:812	arg1	liver					777:781	liver	777:781	liver	777:781	Transcriptome sequencing of liver and 16 S-seq of cecal contents were also performed to explore the related mechanisms of H2 inhalation.					
36997047	12	5	theme	Bacteroidetes-to-Firmicutes	1730:1756	arg1	abundance					1717:1725	the relative abundance	1704:1725	the relative abundance of Bacteroidetes-to-Firmicutes	1704:1756	Based on 16 S rRNA sequencing, H2 altered the composition of gut microbiota, improving the relative abundance of Bacteroidetes-to-Firmicutes.					
36997047	6	6	from	effects	616:622	arg1	markers					683:689	inflammatory markers	670:689	inflammatory markers	670:689	The protective effects on hepatic histopathology, glucose tolerance, inflammatory markers, and intestinal epithelial tight junctions were assessed.					
36997047	6	6	from	effects	616:622	arg1	junctions					724:732	intestinal epithelial tight junctions	696:732	intestinal epithelial tight junctions	696:732	The protective effects on hepatic histopathology, glucose tolerance, inflammatory markers, and intestinal epithelial tight junctions were assessed.					
36997047	6	6	from	effects	616:622	arg1	histopathology					635:648	hepatic histopathology	627:648	hepatic histopathology	627:648	The protective effects on hepatic histopathology, glucose tolerance, inflammatory markers, and intestinal epithelial tight junctions were assessed.					
36997047	6	6	from	effects	616:622	arg1	tolerance					659:667	glucose tolerance	651:667	glucose tolerance	651:667	The protective effects on hepatic histopathology, glucose tolerance, inflammatory markers, and intestinal epithelial tight junctions were assessed.					
36997047	13	7	theme	pathway	1965:1971	arg1	inhibition					1923:1932	inhibition	1923:1932	inhibition of LPS/TLR4/NF-κB inflammatory pathway	1923:1971	Collectively, our data show that H2 could prevent NAFLD induced by high-fat diet, and the anti-NAFLD effect is associated with the modulation of gut microbiota and inhibition of LPS/TLR4/NF-κB inflammatory pathway.					
36997047	13	7	theme	pathway	1965:1971	arg1	modulation					1890:1899	the modulation	1886:1899	the modulation of gut microbiota	1886:1917	Collectively, our data show that H2 could prevent NAFLD induced by high-fat diet, and the anti-NAFLD effect is associated with the modulation of gut microbiota and inhibition of LPS/TLR4/NF-κB inflammatory pathway.					
36997047	8	8	theme	function	974:981	arg1	parameters					983:992	the liver function parameters	964:992	the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase	964:1058	H2 improved the hepatic histological changes and glucose tolerance, decreased the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase, and relieved liver inflammation.					
36997047	0	9	theme	liver	137:141	arg1	disease					143:149	high-fat diet-induced non-alcoholic fatty liver disease	95:149	high-fat diet-induced non-alcoholic fatty liver disease	95:149	Hydrogen inhalation ameliorates hepatic inflammation and modulates gut microbiota in rats with high-fat diet-induced non-alcoholic fatty liver disease.					
36997047	1	10	theme	metabolic	210:218	arg1	disease					177:183	Nonalcoholic fatty liver disease	152:183	Nonalcoholic fatty liver disease (NAFLD)	152:191	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	1	10	theme	metabolic	210:218	arg1	disease					220:226	a multisystem metabolic disease	196:226	a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation	196:284	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	0	11	from	microbiota	71:80	arg1	rats					85:88	rats	85:88	rats with high-fat diet-induced non-alcoholic fatty liver disease	85:149	Hydrogen inhalation ameliorates hepatic inflammation and modulates gut microbiota in rats with high-fat diet-induced non-alcoholic fatty liver disease.					
36997047	12	12	theme	relative	1708:1715	arg1	abundance					1717:1725	the relative abundance	1704:1725	the relative abundance of Bacteroidetes-to-Firmicutes	1704:1756	Based on 16 S rRNA sequencing, H2 altered the composition of gut microbiota, improving the relative abundance of Bacteroidetes-to-Firmicutes.					
36997047	9	13	theme	transcription	1273:1285	arg1	NF-κB					1303:1307	NF-κB	1303:1307	NF-κB	1303:1307	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	9	13	theme	transcription	1273:1285	arg1	B					1300:1300	lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B	1213:1300	the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway	1209:1326	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	1	14	theme	Nonalcoholic	152:163	arg1	disease					220:226	a multisystem metabolic disease	196:226	a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation	196:284	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	1	14	theme	Nonalcoholic	152:163	arg1	NAFLD					186:190	NAFLD	186:190	NAFLD	186:190	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	1	14	theme	Nonalcoholic	152:163	arg1	disease					177:183	Nonalcoholic fatty liver disease	152:183	Nonalcoholic fatty liver disease (NAFLD)	152:191	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	9	15	theme	kappa	1294:1298	arg1	NF-κB					1303:1307	NF-κB	1303:1307	NF-κB	1303:1307	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	9	15	theme	kappa	1294:1298	arg1	B					1300:1300	lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B	1213:1300	the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway	1209:1326	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	1	16	theme	liver	171:175	arg1	disease					220:226	a multisystem metabolic disease	196:226	a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation	196:284	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	1	16	theme	liver	171:175	arg1	NAFLD					186:190	NAFLD	186:190	NAFLD	186:190	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	1	16	theme	liver	171:175	arg1	disease					177:183	Nonalcoholic fatty liver disease	152:183	Nonalcoholic fatty liver disease (NAFLD)	152:191	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	12	17	theme	microbiota	1682:1691	arg1	composition					1663:1673	the composition	1659:1673	the composition of gut microbiota	1659:1691	Based on 16 S rRNA sequencing, H2 altered the composition of gut microbiota, improving the relative abundance of Bacteroidetes-to-Firmicutes.					
36997047	4	18	theme	Sprague-Dawley	470:483	arg1	rats					485:488	Sprague-Dawley rats	470:488	Sprague-Dawley rats	470:488	Sprague-Dawley rats were fed a high-fat diet for 10 weeks to induce NAFLD.					
36997047	11	19	theme	intestinal	1517:1526	arg1	barrier					1543:1549	the intestinal tight junction barrier	1513:1549	the intestinal tight junction barrier	1513:1549	H2 also improved the intestinal tight junction barrier by enhancing the expressions of zonula occludens-1 and occluding.					
36997047	5	20	theme	treatment	553:561	arg1	group					563:567	treatment group	553:567	treatment group	553:567	Rats in treatment group inhaled 4% H2 each day for 2 h.					
36997047	10	21	theme	LPS	1434:1436	arg1	level					1438:1442	the plasma LPS level	1423:1442	the plasma LPS level	1423:1442	Meanwhile, the plasma LPS level was significantly decreased by the H2 intervention.					
36997047	10	21	theme	LPS	1434:1436	arg1	Meanwhile					1412:1420	Meanwhile	1412:1420	Meanwhile	1412:1420	Meanwhile, the plasma LPS level was significantly decreased by the H2 intervention.					
36997047	13	22	theme	gut	1904:1906	arg1	microbiota					1908:1917	gut microbiota	1904:1917	gut microbiota	1904:1917	Collectively, our data show that H2 could prevent NAFLD induced by high-fat diet, and the anti-NAFLD effect is associated with the modulation of gut microbiota and inhibition of LPS/TLR4/NF-κB inflammatory pathway.					
36997047	0	23	theme	high-fat	95:102	arg1	disease					143:149	high-fat diet-induced non-alcoholic fatty liver disease	95:149	high-fat diet-induced non-alcoholic fatty liver disease	95:149	Hydrogen inhalation ameliorates hepatic inflammation and modulates gut microbiota in rats with high-fat diet-induced non-alcoholic fatty liver disease.					
36997047	8	24	theme	alanine	1004:1010	arg1	aminotransferase					1012:1027	plasma alanine aminotransferase	997:1027	plasma alanine aminotransferase	997:1027	H2 improved the hepatic histological changes and glucose tolerance, decreased the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase, and relieved liver inflammation.					
36997047	3	25	theme	present	354:360	arg1	study					362:366	The present study	350:366	The present study	350:366	The present study was aimed to clarify the effects of 4% hydrogen (H2) inhalation on NAFLD and its mechanism of action.					
36997047	6	26	theme	glucose	651:657	arg1	tolerance					659:667	glucose tolerance	651:667	glucose tolerance	651:667	The protective effects on hepatic histopathology, glucose tolerance, inflammatory markers, and intestinal epithelial tight junctions were assessed.					
36997047	0	27	theme	non-alcoholic	117:129	arg1	disease					143:149	high-fat diet-induced non-alcoholic fatty liver disease	95:149	high-fat diet-induced non-alcoholic fatty liver disease	95:149	Hydrogen inhalation ameliorates hepatic inflammation and modulates gut microbiota in rats with high-fat diet-induced non-alcoholic fatty liver disease.					
36997047	0	28	theme	Hydrogen	0:7	arg1	inhalation					9:18	Hydrogen inhalation	0:18	Hydrogen inhalation	0:18	Hydrogen inhalation ameliorates hepatic inflammation and modulates gut microbiota in rats with high-fat diet-induced non-alcoholic fatty liver disease.					
36997047	8	29	theme	aminotransferase	1043:1058	arg1	parameters					983:992	the liver function parameters	964:992	the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase	964:1058	H2 improved the hepatic histological changes and glucose tolerance, decreased the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase, and relieved liver inflammation.					
36997047	7	30	theme	Transcriptome	749:761	arg1	sequencing					763:772	Transcriptome sequencing	749:772	Transcriptome sequencing of liver and 16 S-seq of cecal contents	749:812	Transcriptome sequencing of liver and 16 S-seq of cecal contents were also performed to explore the related mechanisms of H2 inhalation.					
36997047	13	31	theme	anti-NAFLD	1849:1858	arg1	effect					1860:1865	the anti-NAFLD effect	1845:1865	the anti-NAFLD effect	1845:1865	Collectively, our data show that H2 could prevent NAFLD induced by high-fat diet, and the anti-NAFLD effect is associated with the modulation of gut microbiota and inhibition of LPS/TLR4/NF-κB inflammatory pathway.					
36997047	3	32	theme	%	405:405	arg1	inhalation					421:430	4% hydrogen (H2) inhalation	404:430	4% hydrogen (H2) inhalation	404:430	The present study was aimed to clarify the effects of 4% hydrogen (H2) inhalation on NAFLD and its mechanism of action.					
36997047	6	33	theme	protective	605:614	arg1	effects					616:622	The protective effects	601:622	The protective effects on hepatic histopathology, glucose tolerance, inflammatory markers, and intestinal epithelial tight junctions	601:732	The protective effects on hepatic histopathology, glucose tolerance, inflammatory markers, and intestinal epithelial tight junctions were assessed.					
36997047	8	34	theme	liver	1074:1078	arg1	inflammation					1080:1091	liver inflammation	1074:1091	liver inflammation	1074:1091	H2 improved the hepatic histological changes and glucose tolerance, decreased the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase, and relieved liver inflammation.					
36997047	11	35	theme	occluding	1606:1614	arg1	expressions					1568:1578	the expressions	1564:1578	the expressions of zonula occludens-1 and occluding	1564:1614	H2 also improved the intestinal tight junction barrier by enhancing the expressions of zonula occludens-1 and occluding.					
36997047	3	36	theme	action	462:467	arg1	NAFLD					435:439	NAFLD	435:439	NAFLD	435:439	The present study was aimed to clarify the effects of 4% hydrogen (H2) inhalation on NAFLD and its mechanism of action.					
36997047	3	36	theme	action	462:467	arg1	mechanism					449:457	its mechanism	445:457	its mechanism of action	445:467	The present study was aimed to clarify the effects of 4% hydrogen (H2) inhalation on NAFLD and its mechanism of action.					
36997047	11	37	theme	occludens-1	1590:1600	arg1	expressions					1568:1578	the expressions	1564:1578	the expressions of zonula occludens-1 and occluding	1564:1614	H2 also improved the intestinal tight junction barrier by enhancing the expressions of zonula occludens-1 and occluding.					
36997047	7	38	theme	inhalation	874:883	arg1	mechanisms					857:866	the related mechanisms	845:866	the related mechanisms of H2 inhalation	845:883	Transcriptome sequencing of liver and 16 S-seq of cecal contents were also performed to explore the related mechanisms of H2 inhalation.					
36997047	9	39	theme	proteins	1379:1386	arg1	expressions					1355:1365	the expressions	1351:1365	the expressions of critical proteins	1351:1386	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	8	40	theme	histological	910:921	arg1	changes					923:929	the hepatic histological changes	898:929	the hepatic histological changes	898:929	H2 improved the hepatic histological changes and glucose tolerance, decreased the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase, and relieved liver inflammation.					
36997047	13	41	theme	high-fat	1826:1833	arg1	diet					1835:1838	high-fat diet	1826:1838	high-fat diet	1826:1838	Collectively, our data show that H2 could prevent NAFLD induced by high-fat diet, and the anti-NAFLD effect is associated with the modulation of gut microbiota and inhibition of LPS/TLR4/NF-κB inflammatory pathway.					
36997047	9	42	theme	response	1189:1196	arg1	genes					1198:1202	inflammatory response genes	1176:1202	inflammatory response genes	1176:1202	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	7	43	theme	related	849:855	arg1	mechanisms					857:866	the related mechanisms	845:866	the related mechanisms of H2 inhalation	845:883	Transcriptome sequencing of liver and 16 S-seq of cecal contents were also performed to explore the related mechanisms of H2 inhalation.					
36997047	0	44	with	rats	85:88	arg1	disease					143:149	high-fat diet-induced non-alcoholic fatty liver disease	95:149	high-fat diet-induced non-alcoholic fatty liver disease	95:149	Hydrogen inhalation ameliorates hepatic inflammation and modulates gut microbiota in rats with high-fat diet-induced non-alcoholic fatty liver disease.					
36997047	6	45	theme	epithelial	707:716	arg1	junctions					724:732	intestinal epithelial tight junctions	696:732	intestinal epithelial tight junctions	696:732	The protective effects on hepatic histopathology, glucose tolerance, inflammatory markers, and intestinal epithelial tight junctions were assessed.					
36997047	9	46	dep	4/nuclear	1263:1271	arg1	LPS					1233:1235	LPS	1233:1235	LPS	1233:1235	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	9	46	dep	4/nuclear	1263:1271	arg1	lipopolysaccharide					1213:1230	lipopolysaccharide	1213:1230	lipopolysaccharide (LPS)	1213:1236	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	5	47	theme	%	578:578	arg1	H2					580:581	4% H2	577:581	4% H2 each day for 2 h	577:598	Rats in treatment group inhaled 4% H2 each day for 2 h.					
36997047	11	48	theme	junction	1534:1541	arg1	barrier					1543:1549	the intestinal tight junction barrier	1513:1549	the intestinal tight junction barrier	1513:1549	H2 also improved the intestinal tight junction barrier by enhancing the expressions of zonula occludens-1 and occluding.					
36997047	5	49	dep	H2	580:581	arg1	day					588:590	each day	583:590	4% H2 each day for 2 h	577:598	Rats in treatment group inhaled 4% H2 each day for 2 h.					
36997047	8	50	theme	liver	968:972	arg1	parameters					983:992	the liver function parameters	964:992	the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase	964:1058	H2 improved the hepatic histological changes and glucose tolerance, decreased the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase, and relieved liver inflammation.					
36997047	13	51	theme	inflammatory	1952:1963	arg1	pathway					1965:1971	LPS/TLR4/NF-κB inflammatory pathway	1937:1971	LPS/TLR4/NF-κB inflammatory pathway	1937:1971	Collectively, our data show that H2 could prevent NAFLD induced by high-fat diet, and the anti-NAFLD effect is associated with the modulation of gut microbiota and inhibition of LPS/TLR4/NF-κB inflammatory pathway.					
36997047	2	52	theme	effective	316:324	arg1	agent					343:347	a novel and effective antiinflammatory agent	304:347	a novel and effective antiinflammatory agent	304:347	Hydrogen (H2) is a novel and effective antiinflammatory agent.					
36997047	2	52	theme	effective	316:324	arg1	Hydrogen					287:294	Hydrogen	287:294	Hydrogen (H2)	287:299	Hydrogen (H2) is a novel and effective antiinflammatory agent.					
36997047	2	53	theme	novel	306:310	arg1	agent					343:347	a novel and effective antiinflammatory agent	304:347	a novel and effective antiinflammatory agent	304:347	Hydrogen (H2) is a novel and effective antiinflammatory agent.					
36997047	2	53	theme	novel	306:310	arg1	Hydrogen					287:294	Hydrogen	287:294	Hydrogen (H2)	287:299	Hydrogen (H2) is a novel and effective antiinflammatory agent.					
36997047	7	54	theme	cecal	799:803	arg1	contents					805:812	cecal contents	799:812	cecal contents	799:812	Transcriptome sequencing of liver and 16 S-seq of cecal contents were also performed to explore the related mechanisms of H2 inhalation.					
36997047	1	55	theme	multisystem	198:208	arg1	disease					177:183	Nonalcoholic fatty liver disease	152:183	Nonalcoholic fatty liver disease (NAFLD)	152:191	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	1	55	theme	multisystem	198:208	arg1	disease					220:226	a multisystem metabolic disease	196:226	a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation	196:284	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	12	56	theme	16 S	1626:1629	arg1	sequencing					1636:1645	16 S rRNA sequencing	1626:1645	16 S rRNA sequencing	1626:1645	Based on 16 S rRNA sequencing, H2 altered the composition of gut microbiota, improving the relative abundance of Bacteroidetes-to-Firmicutes.					
36997047	6	57	theme	intestinal	696:705	arg1	junctions					724:732	intestinal epithelial tight junctions	696:732	intestinal epithelial tight junctions	696:732	The protective effects on hepatic histopathology, glucose tolerance, inflammatory markers, and intestinal epithelial tight junctions were assessed.					
36997047	1	58	dep	microflora	248:257	arg1	dysbiosis					259:267	dysbiosis	259:267	dysbiosis	259:267	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	7	59	theme	16 S-seq	787:794	arg1	sequencing					763:772	Transcriptome sequencing	749:772	Transcriptome sequencing of liver and 16 S-seq of cecal contents	749:812	Transcriptome sequencing of liver and 16 S-seq of cecal contents were also performed to explore the related mechanisms of H2 inhalation.					
36997047	5	60	from	Rats	545:548	arg1	group					563:567	treatment group	553:567	treatment group	553:567	Rats in treatment group inhaled 4% H2 each day for 2 h.					
36997047	13	61	theme	microbiota	1908:1917	arg1	inhibition					1923:1932	inhibition	1923:1932	inhibition of LPS/TLR4/NF-κB inflammatory pathway	1923:1971	Collectively, our data show that H2 could prevent NAFLD induced by high-fat diet, and the anti-NAFLD effect is associated with the modulation of gut microbiota and inhibition of LPS/TLR4/NF-κB inflammatory pathway.					
36997047	13	61	theme	microbiota	1908:1917	arg1	modulation					1890:1899	the modulation	1886:1899	the modulation of gut microbiota	1886:1917	Collectively, our data show that H2 could prevent NAFLD induced by high-fat diet, and the anti-NAFLD effect is associated with the modulation of gut microbiota and inhibition of LPS/TLR4/NF-κB inflammatory pathway.					
36997047	9	62	theme	4/nuclear	1263:1271	arg1	NF-κB					1303:1307	NF-κB	1303:1307	NF-κB	1303:1307	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	9	62	theme	4/nuclear	1263:1271	arg1	B					1300:1300	lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B	1213:1300	the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway	1209:1326	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	9	63	theme	factor	1287:1292	arg1	NF-κB					1303:1307	NF-κB	1303:1307	NF-κB	1303:1307	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	9	63	theme	factor	1287:1292	arg1	B					1300:1300	lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B	1213:1300	the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway	1209:1326	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	1	64	theme	fatty	165:169	arg1	disease					220:226	a multisystem metabolic disease	196:226	a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation	196:284	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	1	64	theme	fatty	165:169	arg1	NAFLD					186:190	NAFLD	186:190	NAFLD	186:190	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	1	64	theme	fatty	165:169	arg1	disease					177:183	Nonalcoholic fatty liver disease	152:183	Nonalcoholic fatty liver disease (NAFLD)	152:191	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	9	65	theme	B	1300:1300	arg1	pathway					1320:1326	the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway	1209:1326	the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway	1209:1326	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	8	66	theme	plasma	997:1002	arg1	aminotransferase					1012:1027	plasma alanine aminotransferase	997:1027	plasma alanine aminotransferase	997:1027	H2 improved the hepatic histological changes and glucose tolerance, decreased the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase, and relieved liver inflammation.					
36997047	12	67	theme	gut	1678:1680	arg1	microbiota					1682:1691	gut microbiota	1678:1691	gut microbiota	1678:1691	Based on 16 S rRNA sequencing, H2 altered the composition of gut microbiota, improving the relative abundance of Bacteroidetes-to-Firmicutes.					
36997047	0	68	theme	diet-induced	104:115	arg1	disease					143:149	high-fat diet-induced non-alcoholic fatty liver disease	95:149	high-fat diet-induced non-alcoholic fatty liver disease	95:149	Hydrogen inhalation ameliorates hepatic inflammation and modulates gut microbiota in rats with high-fat diet-induced non-alcoholic fatty liver disease.					
36997047	8	69	theme	aminotransferase	1012:1027	arg1	parameters					983:992	the liver function parameters	964:992	the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase	964:1058	H2 improved the hepatic histological changes and glucose tolerance, decreased the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase, and relieved liver inflammation.					
36997047	10	70	theme	plasma	1427:1432	arg1	level					1438:1442	the plasma LPS level	1423:1442	the plasma LPS level	1423:1442	Meanwhile, the plasma LPS level was significantly decreased by the H2 intervention.					
36997047	10	70	theme	plasma	1427:1432	arg1	Meanwhile					1412:1420	Meanwhile	1412:1420	Meanwhile	1412:1420	Meanwhile, the plasma LPS level was significantly decreased by the H2 intervention.					
36997047	0	71	theme	fatty	131:135	arg1	disease					143:149	high-fat diet-induced non-alcoholic fatty liver disease	95:149	high-fat diet-induced non-alcoholic fatty liver disease	95:149	Hydrogen inhalation ameliorates hepatic inflammation and modulates gut microbiota in rats with high-fat diet-induced non-alcoholic fatty liver disease.					
36997047	9	72	theme	signaling	1310:1318	arg1	pathway					1320:1326	the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway	1209:1326	the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway	1209:1326	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	8	73	theme	aspartate	1033:1041	arg1	aminotransferase					1043:1058	aspartate aminotransferase	1033:1058	aspartate aminotransferase	1033:1058	H2 improved the hepatic histological changes and glucose tolerance, decreased the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase, and relieved liver inflammation.					
36997047	5	74	theme	4	577:577	arg1	%					578:578	%	578:578	%	578:578	Rats in treatment group inhaled 4% H2 each day for 2 h.					
36997047	3	75	theme	inhalation	421:430	arg1	effects					393:399	the effects	389:399	the effects of 4% hydrogen (H2) inhalation on NAFLD and its mechanism of action	389:467	The present study was aimed to clarify the effects of 4% hydrogen (H2) inhalation on NAFLD and its mechanism of action.					
36997047	3	76	from	effects	393:399	arg1	NAFLD					435:439	NAFLD	435:439	NAFLD	435:439	The present study was aimed to clarify the effects of 4% hydrogen (H2) inhalation on NAFLD and its mechanism of action.					
36997047	3	76	from	effects	393:399	arg1	mechanism					449:457	its mechanism	445:457	its mechanism of action	445:467	The present study was aimed to clarify the effects of 4% hydrogen (H2) inhalation on NAFLD and its mechanism of action.					
36997047	6	77	theme	hepatic	627:633	arg1	histopathology					635:648	hepatic histopathology	627:648	hepatic histopathology	627:648	The protective effects on hepatic histopathology, glucose tolerance, inflammatory markers, and intestinal epithelial tight junctions were assessed.					
36997047	3	78	theme	H2	417:418	arg1	inhalation					421:430	4% hydrogen (H2) inhalation	404:430	4% hydrogen (H2) inhalation	404:430	The present study was aimed to clarify the effects of 4% hydrogen (H2) inhalation on NAFLD and its mechanism of action.					
36997047	0	79	theme	hepatic	32:38	arg1	inflammation					40:51	hepatic inflammation	32:51	hepatic inflammation	32:51	Hydrogen inhalation ameliorates hepatic inflammation and modulates gut microbiota in rats with high-fat diet-induced non-alcoholic fatty liver disease.					
36997047	7	80	theme	liver	777:781	arg1	sequencing					763:772	Transcriptome sequencing	749:772	Transcriptome sequencing of liver and 16 S-seq of cecal contents	749:812	Transcriptome sequencing of liver and 16 S-seq of cecal contents were also performed to explore the related mechanisms of H2 inhalation.					
36997047	12	81	theme	rRNA	1631:1634	arg1	sequencing					1636:1645	16 S rRNA sequencing	1626:1645	16 S rRNA sequencing	1626:1645	Based on 16 S rRNA sequencing, H2 altered the composition of gut microbiota, improving the relative abundance of Bacteroidetes-to-Firmicutes.					
36997047	9	82	theme	transcriptomic	1100:1113	arg1	data					1115:1118	Liver transcriptomic data	1094:1118	Liver transcriptomic data	1094:1118	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	3	83	theme	hydrogen	407:414	arg1	inhalation					421:430	4% hydrogen (H2) inhalation	404:430	4% hydrogen (H2) inhalation	404:430	The present study was aimed to clarify the effects of 4% hydrogen (H2) inhalation on NAFLD and its mechanism of action.					
36997047	4	84	theme	high-fat	501:508	arg1	diet					510:513	a high-fat diet	499:513	a high-fat diet for 10 weeks to induce NAFLD	499:542	Sprague-Dawley rats were fed a high-fat diet for 10 weeks to induce NAFLD.					
36997047	10	85	theme	H2	1479:1480	arg1	intervention					1482:1493	the H2 intervention	1475:1493	the H2 intervention	1475:1493	Meanwhile, the plasma LPS level was significantly decreased by the H2 intervention.					
36997047	6	86	theme	tight	718:722	arg1	junctions					724:732	intestinal epithelial tight junctions	696:732	intestinal epithelial tight junctions	696:732	The protective effects on hepatic histopathology, glucose tolerance, inflammatory markers, and intestinal epithelial tight junctions were assessed.					
36997047	0	87	theme	gut	67:69	arg1	microbiota					71:80	gut microbiota	67:80	gut microbiota in rats with high-fat diet-induced non-alcoholic fatty liver disease	67:149	Hydrogen inhalation ameliorates hepatic inflammation and modulates gut microbiota in rats with high-fat diet-induced non-alcoholic fatty liver disease.					
36997047	3	88	theme	4	404:404	arg1	%					405:405	%	405:405	%	405:405	The present study was aimed to clarify the effects of 4% hydrogen (H2) inhalation on NAFLD and its mechanism of action.					
36997047	9	89	theme	H2	1135:1136	arg1	treatment					1138:1146	H2 treatment	1135:1146	H2 treatment	1135:1146	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	7	90	theme	H2	871:872	arg1	inhalation					874:883	H2 inhalation	871:883	H2 inhalation	871:883	Transcriptome sequencing of liver and 16 S-seq of cecal contents were also performed to explore the related mechanisms of H2 inhalation.					
36997047	9	91	theme	critical	1370:1377	arg1	proteins					1379:1386	critical proteins	1370:1386	critical proteins	1370:1386	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
36997047	6	92	theme	inflammatory	670:681	arg1	markers					683:689	inflammatory markers	670:689	inflammatory markers	670:689	The protective effects on hepatic histopathology, glucose tolerance, inflammatory markers, and intestinal epithelial tight junctions were assessed.					
36997047	2	93	theme	antiinflammatory	326:341	arg1	agent					343:347	a novel and effective antiinflammatory agent	304:347	a novel and effective antiinflammatory agent	304:347	Hydrogen (H2) is a novel and effective antiinflammatory agent.					
36997047	2	93	theme	antiinflammatory	326:341	arg1	Hydrogen					287:294	Hydrogen	287:294	Hydrogen (H2)	287:299	Hydrogen (H2) is a novel and effective antiinflammatory agent.					
36997047	1	94	theme	gut	244:246	arg1	microflora					248:257	gut microflora dysbiosis and inflammation	244:284	microflora	248:257	Nonalcoholic fatty liver disease (NAFLD) is a multisystem metabolic disease associated with gut microflora dysbiosis and inflammation.					
36997047	8	95	theme	hepatic	902:908	arg1	changes					923:929	the hepatic histological changes	898:929	the hepatic histological changes	898:929	H2 improved the hepatic histological changes and glucose tolerance, decreased the liver function parameters of plasma alanine aminotransferase and aspartate aminotransferase, and relieved liver inflammation.					
36997047	9	96	theme	inflammatory	1176:1187	arg1	genes					1198:1202	inflammatory response genes	1176:1202	inflammatory response genes	1176:1202	Liver transcriptomic data suggested that H2 treatment significantly downregulated inflammatory response genes, and the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4/nuclear transcription factor kappa B (NF-κB) signaling pathway might be involved, and the expressions of critical proteins were further validated.					
35416346	4	0	dep	factors	693:699	arg1	factors					693:699	chondrogenic transcription factors SOX5 and ARID5B	666:715	chondrogenic transcription factors SOX5 and ARID5B	666:715	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	4	0	dep	factors	693:699	arg1	ARID5B					710:715	ARID5B	710:715	ARID5B	710:715	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	4	0	dep	factors	693:699	arg1	SOX5					701:704	SOX5	701:704	SOX5	701:704	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	2	1	theme	human	355:359	arg1	hESCs					383:387	hESCs	383:387	hESCs	383:387	Here we investigate the role of SIRT1 during development of chondrocytes by using human embryonic stem cells (hESCs).					
35416346	2	1	theme	human	355:359	arg1	cells					376:380	human embryonic stem cells	355:380	human embryonic stem cells (hESCs)	355:388	Here we investigate the role of SIRT1 during development of chondrocytes by using human embryonic stem cells (hESCs).					
35416346	7	2	theme	GAG	1431:1433	arg1	content					1435:1441	suppressing GAG content	1419:1441	suppressing GAG content	1419:1441	In conclusion, SIRT1 activation positively impacts on the expression of the main ECM proteins, while altering ECM composition and suppressing GAG content during human cartilage development.					
35416346	1	3	theme	articular	240:248	arg1	homeostasis					260:270	articular cartilage homeostasis	240:270	articular cartilage homeostasis	240:270	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	5	4	theme	responsible	1030:1040	arg1	type					1014:1017	N-acetylgalactosaminyltransferase type 1	980:1019	N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation	980:1076	Unexpectedly, when SIRT1 was activated, while ACAN was enhanced, glycosaminoglycans (GAGs) were reduced, paralleled by down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation.					
35416346	5	4	theme	responsible	1030:1040	arg1	GALNT1					1022:1027	GALNT1	1022:1027	GALNT1	1022:1027	Unexpectedly, when SIRT1 was activated, while ACAN was enhanced, glycosaminoglycans (GAGs) were reduced, paralleled by down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation.					
35416346	8	5	from	effect	1540:1545	arg1	proteins					1559:1566	proteins	1559:1566	proteins	1559:1566	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	8	5	from	effect	1540:1545	arg1	hESC-chondrocytes					1582:1598	developing hESC-chondrocytes	1571:1598	developing hESC-chondrocytes	1571:1598	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	8	5	from	effect	1540:1545	arg1	GAGs					1550:1553	GAGs	1550:1553	GAGs	1550:1553	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	1	6	theme	cartilage	250:258	arg1	homeostasis					260:270	articular cartilage homeostasis	240:270	articular cartilage homeostasis	240:270	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	7	7	theme	human	1450:1454	arg1	development					1466:1476	human cartilage development	1450:1476	human cartilage development	1450:1476	In conclusion, SIRT1 activation positively impacts on the expression of the main ECM proteins, while altering ECM composition and suppressing GAG content during human cartilage development.					
35416346	5	8	theme	expression	965:974	arg1	regulation					946:955	down regulation	941:955	down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation	941:1076	Unexpectedly, when SIRT1 was activated, while ACAN was enhanced, glycosaminoglycans (GAGs) were reduced, paralleled by down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation.					
35416346	4	9	theme	ChIP	728:731	arg1	analysis					733:740	SOX5 ChIP analysis	723:740	SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN	723:819	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	1	10	theme	homeostasis	260:270	arg1	modification					96:107	Epigenetic modification	85:107	Epigenetic modification	85:107	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	10	theme	homeostasis	260:270	arg1	driver					118:123	a key driver	112:123	a key driver of differentiation	112:142	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	10	theme	homeostasis	260:270	arg1	regulator					200:208	an established regulator	185:208	an established regulator of cell function, ageing, and articular cartilage homeostasis	185:270	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	10	theme	homeostasis	260:270	arg1	SIRT1					175:179	the deacetylase Sirtuin1 (SIRT1)	149:180	the deacetylase Sirtuin1 (SIRT1)	149:180	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	5	11	theme	GAG	1046:1048	arg1	initiation/elongation					1056:1076	GAG chain initiation/elongation	1046:1076	GAG chain initiation/elongation	1046:1076	Unexpectedly, when SIRT1 was activated, while ACAN was enhanced, glycosaminoglycans (GAGs) were reduced, paralleled by down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation.					
35416346	1	12	theme	Epigenetic	85:94	arg1	modification					96:107	Epigenetic modification	85:107	Epigenetic modification	85:107	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	12	theme	Epigenetic	85:94	arg1	driver					118:123	a key driver	112:123	a key driver of differentiation	112:142	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	3	13	dep	EX527	473:477	arg1	differentiation					487:501	differentiation	487:501	differentiation	487:501	HESC-chondroprogenitors were treated with SIRT1 activator; SRT1720, or inhibitor; EX527, during differentiation.					
35416346	4	14	theme	factors	693:699	arg1	expression					587:596	the expression	583:596	the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B	583:715	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	4	15	theme	transcription	679:691	arg1	factors					693:699	chondrogenic transcription factors SOX5 and ARID5B	666:715	chondrogenic transcription factors SOX5 and ARID5B	666:715	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	4	15	theme	transcription	679:691	arg1	ARID5B					710:715	ARID5B	710:715	ARID5B	710:715	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	4	15	theme	transcription	679:691	arg1	SOX5					701:704	SOX5	701:704	SOX5	701:704	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	6	16	theme	COL2A1	1227:1232	arg1	expression					1234:1243	COL2A1 expression	1227:1243	COL2A1 expression	1227:1243	A positive correlation between ARID5B and COL2A1 was observed, and co-IP assays indicated association of ARID5B with SIRT1, further suggesting that COL2A1 expression is promoted by an ARID5B-SIRT1 interaction.					
35416346	4	17	theme	type-II	615:621	arg1	COL2A1					633:638	COL2A1	633:638	COL2A1	633:638	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	4	17	theme	type-II	615:621	arg1	collagen					623:630	type-II collagen	615:630	type-II collagen (COL2A1)	615:639	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	8	18	contain	has	1521:1523	arg1	activity					1512:1519	SIRT1 activity	1506:1519	SIRT1 activity	1506:1519	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	8	18	contain	has	1521:1523	arg2	effect					1540:1545	a differential effect	1525:1545	a differential effect on GAGs and proteins in developing hESC-chondrocytes	1525:1598	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	4	19	from	increases	570:578	arg1	expression					587:596	the expression	583:596	the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B	583:715	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	8	20	theme	SIRT1	1506:1510	arg1	activity					1512:1519	SIRT1 activity	1506:1519	SIRT1 activity	1506:1519	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	8	21	theme	developing	1571:1580	arg1	hESC-chondrocytes					1582:1598	developing hESC-chondrocytes	1571:1598	developing hESC-chondrocytes	1571:1598	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	8	22	theme	positive	1710:1717	arg1	mediators					1723:1731	positive GAG mediators	1710:1731	positive GAG mediators	1710:1731	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	7	23	theme	SIRT1	1304:1308	arg1	activation					1310:1319	SIRT1 activation	1304:1319	SIRT1 activation	1304:1319	In conclusion, SIRT1 activation positively impacts on the expression of the main ECM proteins, while altering ECM composition and suppressing GAG content during human cartilage development.					
35416346	5	24	theme	gene	960:963	arg1	expression					965:974	gene expression	960:974	gene expression	960:974	Unexpectedly, when SIRT1 was activated, while ACAN was enhanced, glycosaminoglycans (GAGs) were reduced, paralleled by down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation.					
35416346	7	25	theme	suppressing	1419:1429	arg1	content					1435:1441	suppressing GAG content	1419:1441	suppressing GAG content	1419:1441	In conclusion, SIRT1 activation positively impacts on the expression of the main ECM proteins, while altering ECM composition and suppressing GAG content during human cartilage development.					
35416346	4	26	theme	ACAN	816:819	arg1	enhancer					804:811	the chondrocyte specific -10 (A1) enhancer	770:811	the chondrocyte specific -10 (A1) enhancer of ACAN	770:819	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	8	27	theme	differential	1527:1538	arg1	effect					1540:1545	a differential effect	1525:1545	a differential effect on GAGs and proteins in developing hESC-chondrocytes	1525:1598	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	7	28	theme	proteins	1374:1381	arg1	expression					1347:1356	the expression	1343:1356	the expression of the main ECM proteins	1343:1381	In conclusion, SIRT1 activation positively impacts on the expression of the main ECM proteins, while altering ECM composition and suppressing GAG content during human cartilage development.					
35416346	5	29	theme	chain	1050:1054	arg1	initiation/elongation					1056:1076	GAG chain initiation/elongation	1046:1076	GAG chain initiation/elongation	1046:1076	Unexpectedly, when SIRT1 was activated, while ACAN was enhanced, glycosaminoglycans (GAGs) were reduced, paralleled by down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation.					
35416346	0	30	theme	SIRT1	0:4	arg1	activity					6:13	SIRT1 activity	0:13	SIRT1 activity	0:13	SIRT1 activity orchestrates ECM expression during hESC-chondrogenic differentiation.					
35416346	6	31	with	association	1169:1179	arg1	SIRT1					1196:1200	SIRT1	1196:1200	SIRT1	1196:1200	A positive correlation between ARID5B and COL2A1 was observed, and co-IP assays indicated association of ARID5B with SIRT1, further suggesting that COL2A1 expression is promoted by an ARID5B-SIRT1 interaction.					
35416346	1	32	theme	key	114:116	arg1	modification					96:107	Epigenetic modification	85:107	Epigenetic modification	85:107	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	32	theme	key	114:116	arg1	driver					118:123	a key driver	112:123	a key driver of differentiation	112:142	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	8	33	theme	mediators	1723:1731	arg1	addition					1698:1705	addition	1698:1705	addition of positive GAG mediators	1698:1731	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	1	34	theme	established	188:198	arg1	regulator					200:208	an established regulator	185:208	an established regulator of cell function, ageing, and articular cartilage homeostasis	185:270	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	34	theme	established	188:198	arg1	SIRT1					175:179	the deacetylase Sirtuin1 (SIRT1)	149:180	the deacetylase Sirtuin1 (SIRT1)	149:180	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	0	35	theme	ECM	28:30	arg1	expression					32:41	ECM expression	28:41	ECM expression	28:41	SIRT1 activity orchestrates ECM expression during hESC-chondrogenic differentiation.					
35416346	5	36	theme	down	941:944	arg1	regulation					946:955	down regulation	941:955	down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation	941:1076	Unexpectedly, when SIRT1 was activated, while ACAN was enhanced, glycosaminoglycans (GAGs) were reduced, paralleled by down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation.					
35416346	7	37	theme	main	1365:1368	arg1	proteins					1374:1381	the main ECM proteins	1361:1381	the main ECM proteins	1361:1381	In conclusion, SIRT1 activation positively impacts on the expression of the main ECM proteins, while altering ECM composition and suppressing GAG content during human cartilage development.					
35416346	8	38	theme	GAG	1719:1721	arg1	mediators					1723:1731	positive GAG mediators	1710:1731	positive GAG mediators	1710:1731	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	2	39	theme	SIRT1	305:309	arg1	role					297:300	the role	293:300	the role of SIRT1 during development of chondrocytes	293:344	Here we investigate the role of SIRT1 during development of chondrocytes by using human embryonic stem cells (hESCs).					
35416346	4	40	theme	significant	558:568	arg1	increases					570:578	significant increases	558:578	significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B	558:715	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	7	41	theme	ECM	1370:1372	arg1	proteins					1374:1381	the main ECM proteins	1361:1381	the main ECM proteins	1361:1381	In conclusion, SIRT1 activation positively impacts on the expression of the main ECM proteins, while altering ECM composition and suppressing GAG content during human cartilage development.					
35416346	8	42	theme	protein	1665:1671	arg1	synthesis					1673:1681	matrix protein synthesis	1658:1681	matrix protein synthesis	1658:1681	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	1	43	theme	deacetylase	153:163	arg1	regulator					200:208	an established regulator	185:208	an established regulator of cell function, ageing, and articular cartilage homeostasis	185:270	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	43	theme	deacetylase	153:163	arg1	SIRT1					175:179	the deacetylase Sirtuin1 (SIRT1)	149:180	the deacetylase Sirtuin1 (SIRT1)	149:180	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	4	44	theme	chondrogenic	666:677	arg1	factors					693:699	chondrogenic transcription factors SOX5 and ARID5B	666:715	chondrogenic transcription factors SOX5 and ARID5B	666:715	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	4	44	theme	chondrogenic	666:677	arg1	ARID5B					710:715	ARID5B	710:715	ARID5B	710:715	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	4	44	theme	chondrogenic	666:677	arg1	SOX5					701:704	SOX5	701:704	SOX5	701:704	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	1	45	theme	cell	213:216	arg1	function					218:225	cell function	213:225	cell function	213:225	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	4	46	theme	early	524:528	arg1	SIRT1					518:522	SIRT1	518:522	SIRT1 early in 3D-pellet culture	518:549	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	4	47	theme	SOX5	723:726	arg1	analysis					733:740	SOX5 ChIP analysis	723:740	SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN	723:819	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	3	48	dep	SIRT1	433:437	arg1	activator					439:447	activator	439:447	activator	439:447	HESC-chondroprogenitors were treated with SIRT1 activator; SRT1720, or inhibitor; EX527, during differentiation.					
35416346	1	49	theme	function	218:225	arg1	modification					96:107	Epigenetic modification	85:107	Epigenetic modification	85:107	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	49	theme	function	218:225	arg1	driver					118:123	a key driver	112:123	a key driver of differentiation	112:142	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	49	theme	function	218:225	arg1	regulator					200:208	an established regulator	185:208	an established regulator of cell function, ageing, and articular cartilage homeostasis	185:270	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	49	theme	function	218:225	arg1	SIRT1					175:179	the deacetylase Sirtuin1 (SIRT1)	149:180	the deacetylase Sirtuin1 (SIRT1)	149:180	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	4	50	from	early	524:528	arg1	culture					543:549	3D-pellet culture	533:549	3D-pellet culture	533:549	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	4	51	theme	SIRT1	518:522	arg1	Activation					504:513	Activation	504:513	Activation of SIRT1 early in 3D-pellet culture	504:549	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	4	52	theme	A1	800:801	arg1	enhancer					804:811	the chondrocyte specific -10 (A1) enhancer	770:811	the chondrocyte specific -10 (A1) enhancer of ACAN	770:819	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	8	53	dep	if	1683:1684	arg1	balanced					1686:1693	balanced	1686:1693	balanced	1686:1693	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	1	54	theme	differentiation	128:142	arg1	modification					96:107	Epigenetic modification	85:107	Epigenetic modification	85:107	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	54	theme	differentiation	128:142	arg1	driver					118:123	a key driver	112:123	a key driver of differentiation	112:142	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	54	theme	differentiation	128:142	arg1	regulator					200:208	an established regulator	185:208	an established regulator of cell function, ageing, and articular cartilage homeostasis	185:270	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	54	theme	differentiation	128:142	arg1	SIRT1					175:179	the deacetylase Sirtuin1 (SIRT1)	149:180	the deacetylase Sirtuin1 (SIRT1)	149:180	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	0	55	theme	hESC-chondrogenic	50:66	arg1	differentiation					68:82	hESC-chondrogenic differentiation	50:82	hESC-chondrogenic differentiation	50:82	SIRT1 activity orchestrates ECM expression during hESC-chondrogenic differentiation.					
35416346	4	56	theme	3D-pellet	533:541	arg1	culture					543:549	3D-pellet culture	533:549	3D-pellet culture	533:549	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	5	57	theme	N-acetylgalactosaminyltransferase	980:1012	arg1	type					1014:1017	N-acetylgalactosaminyltransferase type 1	980:1019	N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation	980:1076	Unexpectedly, when SIRT1 was activated, while ACAN was enhanced, glycosaminoglycans (GAGs) were reduced, paralleled by down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation.					
35416346	5	57	theme	N-acetylgalactosaminyltransferase	980:1012	arg1	GALNT1					1022:1027	GALNT1	1022:1027	GALNT1	1022:1027	Unexpectedly, when SIRT1 was activated, while ACAN was enhanced, glycosaminoglycans (GAGs) were reduced, paralleled by down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation.					
35416346	6	58	theme	ARID5B	1184:1189	arg1	association					1169:1179	association	1169:1179	association of ARID5B with SIRT1	1169:1200	A positive correlation between ARID5B and COL2A1 was observed, and co-IP assays indicated association of ARID5B with SIRT1, further suggesting that COL2A1 expression is promoted by an ARID5B-SIRT1 interaction.					
35416346	5	59	dep	reduced	918:924	arg1	paralleled					927:936	paralleled	927:936	paralleled by down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation	927:1076	Unexpectedly, when SIRT1 was activated, while ACAN was enhanced, glycosaminoglycans (GAGs) were reduced, paralleled by down regulation of gene expression for N-acetylgalactosaminyltransferase type 1 (GALNT1) responsible for GAG chain initiation/elongation.					
35416346	2	60	theme	chondrocytes	333:344	arg1	development					318:328	development	318:328	development of chondrocytes	318:344	Here we investigate the role of SIRT1 during development of chondrocytes by using human embryonic stem cells (hESCs).					
35416346	7	61	theme	ECM	1399:1401	arg1	composition					1403:1413	ECM composition	1399:1413	ECM composition	1399:1413	In conclusion, SIRT1 activation positively impacts on the expression of the main ECM proteins, while altering ECM composition and suppressing GAG content during human cartilage development.					
35416346	6	62	theme	ARID5B-SIRT1	1263:1274	arg1	interaction					1276:1286	an ARID5B-SIRT1 interaction	1260:1286	an ARID5B-SIRT1 interaction	1260:1286	A positive correlation between ARID5B and COL2A1 was observed, and co-IP assays indicated association of ARID5B with SIRT1, further suggesting that COL2A1 expression is promoted by an ARID5B-SIRT1 interaction.					
35416346	4	63	theme	specific	786:793	arg1	enhancer					804:811	the chondrocyte specific -10 (A1) enhancer	770:811	the chondrocyte specific -10 (A1) enhancer of ACAN	770:819	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	6	64	theme	positive	1081:1088	arg1	correlation					1090:1100	A positive correlation	1079:1100	A positive correlation between ARID5B and COL2A1	1079:1126	A positive correlation between ARID5B and COL2A1 was observed, and co-IP assays indicated association of ARID5B with SIRT1, further suggesting that COL2A1 expression is promoted by an ARID5B-SIRT1 interaction.					
35416346	8	65	theme	cartilage	1632:1640	arg1	development					1642:1652	cartilage development	1632:1652	cartilage development	1632:1652	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	4	66	theme	ECM	601:603	arg1	genes					605:609	ECM genes	601:609	ECM genes	601:609	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	6	67	theme	co-IP	1146:1150	arg1	assays					1152:1157	co-IP assays	1146:1157	co-IP assays	1146:1157	A positive correlation between ARID5B and COL2A1 was observed, and co-IP assays indicated association of ARID5B with SIRT1, further suggesting that COL2A1 expression is promoted by an ARID5B-SIRT1 interaction.					
35416346	1	68	theme	Sirtuin1	165:172	arg1	regulator					200:208	an established regulator	185:208	an established regulator of cell function, ageing, and articular cartilage homeostasis	185:270	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	68	theme	Sirtuin1	165:172	arg1	SIRT1					175:179	the deacetylase Sirtuin1 (SIRT1)	149:180	the deacetylase Sirtuin1 (SIRT1)	149:180	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	8	69	theme	matrix	1658:1663	arg1	synthesis					1673:1681	matrix protein synthesis	1658:1681	matrix protein synthesis	1658:1681	These results suggest that SIRT1 activity has a differential effect on GAGs and proteins in developing hESC-chondrocytes and could only be beneficial to cartilage development and matrix protein synthesis if balanced by addition of positive GAG mediators.					
35416346	1	70	theme	ageing	228:233	arg1	modification					96:107	Epigenetic modification	85:107	Epigenetic modification	85:107	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	70	theme	ageing	228:233	arg1	driver					118:123	a key driver	112:123	a key driver of differentiation	112:142	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	70	theme	ageing	228:233	arg1	regulator					200:208	an established regulator	185:208	an established regulator of cell function, ageing, and articular cartilage homeostasis	185:270	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	1	70	theme	ageing	228:233	arg1	SIRT1					175:179	the deacetylase Sirtuin1 (SIRT1)	149:180	the deacetylase Sirtuin1 (SIRT1)	149:180	Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis.					
35416346	4	71	theme	chondrocyte	774:784	arg1	enhancer					804:811	the chondrocyte specific -10 (A1) enhancer	770:811	the chondrocyte specific -10 (A1) enhancer of ACAN	770:819	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	2	72	theme	stem	371:374	arg1	hESCs					383:387	hESCs	383:387	hESCs	383:387	Here we investigate the role of SIRT1 during development of chondrocytes by using human embryonic stem cells (hESCs).					
35416346	2	72	theme	stem	371:374	arg1	cells					376:380	human embryonic stem cells	355:380	human embryonic stem cells (hESCs)	355:388	Here we investigate the role of SIRT1 during development of chondrocytes by using human embryonic stem cells (hESCs).					
35416346	7	73	theme	cartilage	1456:1464	arg1	development					1466:1476	human cartilage development	1450:1476	human cartilage development	1450:1476	In conclusion, SIRT1 activation positively impacts on the expression of the main ECM proteins, while altering ECM composition and suppressing GAG content during human cartilage development.					
35416346	4	74	from	culture	543:549	arg1	early					524:528	early	524:528	early	524:528	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35416346	2	75	theme	embryonic	361:369	arg1	hESCs					383:387	hESCs	383:387	hESCs	383:387	Here we investigate the role of SIRT1 during development of chondrocytes by using human embryonic stem cells (hESCs).					
35416346	2	75	theme	embryonic	361:369	arg1	cells					376:380	human embryonic stem cells	355:380	human embryonic stem cells (hESCs)	355:388	Here we investigate the role of SIRT1 during development of chondrocytes by using human embryonic stem cells (hESCs).					
35416346	4	76	theme	genes	605:609	arg1	expression					587:596	the expression	583:596	the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B	583:715	Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN.					
35294989	3	0	from	pathways	661:668	arg1	microbiota					689:698	the microbiota	685:698	the microbiota	685:698	Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota.					
35294989	3	1	theme	parameter	497:505	arg1	analysis					507:514	Skin parameter analysis	492:514	Skin parameter analysis	492:514	Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota.					
35294989	6	2	from	increase	1087:1094	arg1	group					1117:1121	the CTP/GOS mixed group	1099:1121	the CTP/GOS mixed group	1099:1121	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	3	3	theme	molecular	542:550	arg1	techniques					560:569	molecular biology techniques	542:569	molecular biology techniques	542:569	Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota.					
35294989	1	4	theme	collagen	202:209	arg1	homeostasis					211:221	collagen homeostasis	202:221	collagen homeostasis	202:221	Collagen-tripeptide (CTP) and galacto-oligosaccharide (GOS), which improve collagen homeostasis and barrier function in the skin, are widely used in the food industry to improve wrinkle-related parameters and skin health.					
35294989	3	5	from	changes	674:680	arg1	microbiota					689:698	the microbiota	685:698	the microbiota	685:698	Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota.					
35294989	3	6	theme	HPLC	575:578	arg1	analysis					580:587	HPLC analysis	575:587	HPLC analysis	575:587	Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota.					
35294989	8	7	theme	photoprotective	1478:1492	arg1	activity					1494:1501	photoprotective activity	1478:1501	photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition	1478:1595	Taken together, CTP/GOS mixtures showed a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition.					
35294989	7	8	theme	UV-irradiated	1385:1397	arg1	group					1407:1411	the UV-irradiated control group	1381:1411	the UV-irradiated control group	1381:1411	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	1	9	from	homeostasis	211:221	arg1	skin					251:254	the skin	247:254	the skin	247:254	Collagen-tripeptide (CTP) and galacto-oligosaccharide (GOS), which improve collagen homeostasis and barrier function in the skin, are widely used in the food industry to improve wrinkle-related parameters and skin health.					
35294989	5	10	theme	extracellular	939:951	arg1	expression					973:982	the extracellular matrix-related gene expression	935:982	the extracellular matrix-related gene expression	935:982	Administration of the 1:3 mixture showed significant changes in the extracellular matrix-related gene expression compared to other mixture groups.					
35294989	5	11	theme	gene	968:971	arg1	expression					973:982	the extracellular matrix-related gene expression	935:982	the extracellular matrix-related gene expression	935:982	Administration of the 1:3 mixture showed significant changes in the extracellular matrix-related gene expression compared to other mixture groups.					
35294989	2	12	theme	hairless	464:471	arg1	mice					473:476	ultraviolet (UV) B-irradiated hairless mice	434:476	ultraviolet (UV) B-irradiated hairless mice	434:476	In this study, the photoprotective effect of CTP/GOS mixtures (3:1, 1:1, and 1:3) in ultraviolet (UV) B-irradiated hairless mice was examined.					
35294989	0	13	theme	Photoaging	75:84	arg1	Inhibition					86:95	Skin Photoaging Inhibition	70:95	Skin Photoaging Inhibition in UVB-exposed Hairless Mice	70:124	Effects of Collagen-Tripeptide and Galacto-oligosaccharide Mixture on Skin Photoaging Inhibition in UVB-exposed Hairless Mice.					
35294989	1	14	theme	skin	336:339	arg1	health					341:346	skin health	336:346	skin health	336:346	Collagen-tripeptide (CTP) and galacto-oligosaccharide (GOS), which improve collagen homeostasis and barrier function in the skin, are widely used in the food industry to improve wrinkle-related parameters and skin health.					
35294989	0	15	theme	Collagen-Tripeptide	11:29	arg1	Mixture					59:65	Collagen-Tripeptide and Galacto-oligosaccharide Mixture	11:65	Collagen-Tripeptide and Galacto-oligosaccharide Mixture	11:65	Effects of Collagen-Tripeptide and Galacto-oligosaccharide Mixture on Skin Photoaging Inhibition in UVB-exposed Hairless Mice.					
35294989	5	16	theme	significant	912:922	arg1	changes					924:930	significant changes	912:930	significant changes in the extracellular matrix-related gene expression	912:982	Administration of the 1:3 mixture showed significant changes in the extracellular matrix-related gene expression compared to other mixture groups.					
35294989	8	17	theme	gene	1526:1529	arg1	expression					1531:1540	the gene expression	1522:1540	the gene expression	1522:1540	Taken together, CTP/GOS mixtures showed a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition.					
35294989	7	18	theme	relative	1229:1236	arg1	higher					1323:1328	higher	1323:1328	higher	1323:1328	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	7	18	theme	relative	1229:1236	arg1	ratio					1248:1252	the relative abundance ratio	1225:1252	the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum	1225:1317	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	5	19	from	changes	924:930	arg1	expression					973:982	the extracellular matrix-related gene expression	935:982	the extracellular matrix-related gene expression	935:982	Administration of the 1:3 mixture showed significant changes in the extracellular matrix-related gene expression compared to other mixture groups.					
35294989	6	20	theme	fatty	1040:1044	arg1	SCFA					1052:1055	SCFA	1052:1055	SCFA	1052:1055	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	6	20	theme	fatty	1040:1044	arg1	acid					1046:1049	short-chain fatty acid	1028:1049	The cecal short-chain fatty acid (SCFA) content	1018:1064	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	0	21	from	Effects	0:6	arg1	Inhibition					86:95	Skin Photoaging Inhibition	70:95	Skin Photoaging Inhibition in UVB-exposed Hairless Mice	70:124	Effects of Collagen-Tripeptide and Galacto-oligosaccharide Mixture on Skin Photoaging Inhibition in UVB-exposed Hairless Mice.					
35294989	4	22	theme	physical	763:770	arg1	parameters					772:781	photoaged physical parameters	753:781	photoaged physical parameters	753:781	Oral administration of CTP/GOS mixtures ameliorated photoaged physical parameters and serum levels of pro-inflammatory cytokines compared to UV-irradiated control group.					
35294989	0	23	theme	Hairless	112:119	arg1	Mice					121:124	UVB-exposed Hairless Mice	100:124	UVB-exposed Hairless Mice	100:124	Effects of Collagen-Tripeptide and Galacto-oligosaccharide Mixture on Skin Photoaging Inhibition in UVB-exposed Hairless Mice.					
35294989	6	24	theme	cecal	1022:1026	arg1	content					1058:1064	The cecal short-chain fatty acid (SCFA) content	1018:1064	The cecal short-chain fatty acid (SCFA) content	1018:1064	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	2	25	theme	CTP/GOS	394:400	arg1	mixtures					402:409	CTP/GOS mixtures	394:409	CTP/GOS mixtures (3:1, 1:1, and 1:3)	394:429	In this study, the photoprotective effect of CTP/GOS mixtures (3:1, 1:1, and 1:3) in ultraviolet (UV) B-irradiated hairless mice was examined.					
35294989	7	26	theme	cecal	1207:1211	arg1	microbiota					1213:1222	cecal microbiota	1207:1222	cecal microbiota	1207:1222	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	4	27	theme	pro-inflammatory	803:818	arg1	cytokines					820:828	pro-inflammatory cytokines	803:828	pro-inflammatory cytokines	803:828	Oral administration of CTP/GOS mixtures ameliorated photoaged physical parameters and serum levels of pro-inflammatory cytokines compared to UV-irradiated control group.					
35294989	6	28	theme	CTP/GOS	1103:1109	arg1	group					1117:1121	the CTP/GOS mixed group	1099:1121	the CTP/GOS mixed group	1099:1121	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	8	29	theme	intestinal	1563:1572	arg1	composition					1585:1595	intestinal microbiota composition	1563:1595	intestinal microbiota composition	1563:1595	Taken together, CTP/GOS mixtures showed a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition.					
35294989	4	30	theme	CTP/GOS	724:730	arg1	mixtures					732:739	CTP/GOS mixtures	724:739	CTP/GOS mixtures	724:739	Oral administration of CTP/GOS mixtures ameliorated photoaged physical parameters and serum levels of pro-inflammatory cytokines compared to UV-irradiated control group.					
35294989	5	31	dep	showed	905:910	arg1	compared					984:991	compared	984:991	showed significant changes in the extracellular matrix-related gene expression compared to other mixture groups	905:1015	Administration of the 1:3 mixture showed significant changes in the extracellular matrix-related gene expression compared to other mixture groups.					
35294989	7	32	theme	16S	1180:1182	arg1	analysis					1195:1202	the 16S rRNA-based analysis	1176:1202	the 16S rRNA-based analysis of cecal microbiota	1176:1222	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	5	33	theme	other	996:1000	arg1	groups					1010:1015	other mixture groups	996:1015	other mixture groups	996:1015	Administration of the 1:3 mixture showed significant changes in the extracellular matrix-related gene expression compared to other mixture groups.					
35294989	6	34	theme	significant	1075:1085	arg1	increase					1087:1094	a significant increase	1073:1094	a significant increase in the CTP/GOS mixed group	1073:1121	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	3	35	theme	protective	632:641	arg1	effect					643:648	the photoaging protective effect	617:648	the photoaging protective effect	617:648	Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota.					
35294989	0	36	theme	Galacto-oligosaccharide	35:57	arg1	Mixture					59:65	Collagen-Tripeptide and Galacto-oligosaccharide Mixture	11:65	Collagen-Tripeptide and Galacto-oligosaccharide Mixture	11:65	Effects of Collagen-Tripeptide and Galacto-oligosaccharide Mixture on Skin Photoaging Inhibition in UVB-exposed Hairless Mice.					
35294989	2	37	from	effect	384:389	arg1	mice					473:476	ultraviolet (UV) B-irradiated hairless mice	434:476	ultraviolet (UV) B-irradiated hairless mice	434:476	In this study, the photoprotective effect of CTP/GOS mixtures (3:1, 1:1, and 1:3) in ultraviolet (UV) B-irradiated hairless mice was examined.					
35294989	4	38	theme	UV-irradiated	842:854	arg1	group					864:868	UV-irradiated control group	842:868	UV-irradiated control group	842:868	Oral administration of CTP/GOS mixtures ameliorated photoaged physical parameters and serum levels of pro-inflammatory cytokines compared to UV-irradiated control group.					
35294989	7	39	theme	Verrucomicrobia	1296:1310	arg1	phylum					1312:1317	the Verrucomicrobia phylum	1292:1317	the Verrucomicrobia phylum	1292:1317	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	4	40	theme	cytokines	820:828	arg1	parameters					772:781	photoaged physical parameters	753:781	photoaged physical parameters	753:781	Oral administration of CTP/GOS mixtures ameliorated photoaged physical parameters and serum levels of pro-inflammatory cytokines compared to UV-irradiated control group.					
35294989	4	40	theme	cytokines	820:828	arg1	levels					793:798	serum levels	787:798	serum levels	787:798	Oral administration of CTP/GOS mixtures ameliorated photoaged physical parameters and serum levels of pro-inflammatory cytokines compared to UV-irradiated control group.					
35294989	6	41	theme	GOS	1137:1139	arg1	content					1141:1147	a higher GOS content	1128:1147	a higher GOS content than the control group	1128:1170	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	3	42	from	effect	643:648	arg1	microbiota					689:698	the microbiota	685:698	the microbiota	685:698	Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota.					
35294989	8	43	theme	synergistic	1456:1466	arg1	effect					1468:1473	a synergistic effect	1454:1473	a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition	1454:1595	Taken together, CTP/GOS mixtures showed a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition.					
35294989	7	44	theme	genus	1273:1277	arg1	higher					1323:1328	higher	1323:1328	higher	1323:1328	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	7	44	theme	genus	1273:1277	arg1	ratio					1248:1252	the relative abundance ratio	1225:1252	the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum	1225:1317	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	8	45	theme	CTP/GOS	1430:1436	arg1	mixtures					1438:1445	CTP/GOS mixtures	1430:1445	CTP/GOS mixtures	1430:1445	Taken together, CTP/GOS mixtures showed a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition.					
35294989	3	46	theme	Skin	492:495	arg1	analysis					507:514	Skin parameter analysis	492:514	Skin parameter analysis	492:514	Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota.					
35294989	4	47	theme	control	856:862	arg1	group					864:868	UV-irradiated control group	842:868	UV-irradiated control group	842:868	Oral administration of CTP/GOS mixtures ameliorated photoaged physical parameters and serum levels of pro-inflammatory cytokines compared to UV-irradiated control group.					
35294989	8	48	from	effect	1468:1473	arg1	activity					1494:1501	photoprotective activity	1478:1501	photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition	1478:1595	Taken together, CTP/GOS mixtures showed a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition.					
35294989	5	49	theme	1:3	893:895	arg1	mixture					897:903	the 1:3 mixture	889:903	the 1:3 mixture	889:903	Administration of the 1:3 mixture showed significant changes in the extracellular matrix-related gene expression compared to other mixture groups.					
35294989	3	50	theme	histological	517:528	arg1	approaches					530:539	histological approaches	517:539	histological approaches	517:539	Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota.					
35294989	6	51	theme	control	1158:1164	arg1	group					1166:1170	the control group	1154:1170	the control group	1154:1170	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	5	52	theme	mixture	897:903	arg1	Administration					871:884	Administration	871:884	Administration of the 1:3 mixture	871:903	Administration of the 1:3 mixture showed significant changes in the extracellular matrix-related gene expression compared to other mixture groups.					
35294989	1	53	theme	barrier	227:233	arg1	function					235:242	barrier function	227:242	barrier function	227:242	Collagen-tripeptide (CTP) and galacto-oligosaccharide (GOS), which improve collagen homeostasis and barrier function in the skin, are widely used in the food industry to improve wrinkle-related parameters and skin health.					
35294989	0	54	from	Inhibition	86:95	arg1	Mice					121:124	UVB-exposed Hairless Mice	100:124	UVB-exposed Hairless Mice	100:124	Effects of Collagen-Tripeptide and Galacto-oligosaccharide Mixture on Skin Photoaging Inhibition in UVB-exposed Hairless Mice.					
35294989	3	55	theme	biology	552:558	arg1	techniques					560:569	molecular biology techniques	542:569	molecular biology techniques	542:569	Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota.					
35294989	1	56	from	function	235:242	arg1	skin					251:254	the skin	247:254	the skin	247:254	Collagen-tripeptide (CTP) and galacto-oligosaccharide (GOS), which improve collagen homeostasis and barrier function in the skin, are widely used in the food industry to improve wrinkle-related parameters and skin health.					
35294989	7	57	theme	control	1399:1405	arg1	group					1407:1411	the UV-irradiated control group	1381:1411	the UV-irradiated control group	1381:1411	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	5	58	theme	matrix-related	953:966	arg1	expression					973:982	the extracellular matrix-related gene expression	935:982	the extracellular matrix-related gene expression	935:982	Administration of the 1:3 mixture showed significant changes in the extracellular matrix-related gene expression compared to other mixture groups.					
35294989	4	59	theme	Oral	701:704	arg1	administration					706:719	Oral administration	701:719	Oral administration of CTP/GOS mixtures	701:739	Oral administration of CTP/GOS mixtures ameliorated photoaged physical parameters and serum levels of pro-inflammatory cytokines compared to UV-irradiated control group.					
35294989	7	60	theme	abundance	1238:1246	arg1	higher					1323:1328	higher	1323:1328	higher	1323:1328	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	7	60	theme	abundance	1238:1246	arg1	ratio					1248:1252	the relative abundance ratio	1225:1252	the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum	1225:1317	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	2	61	theme	B-irradiated	451:462	arg1	mice					473:476	ultraviolet (UV) B-irradiated hairless mice	434:476	ultraviolet (UV) B-irradiated hairless mice	434:476	In this study, the photoprotective effect of CTP/GOS mixtures (3:1, 1:1, and 1:3) in ultraviolet (UV) B-irradiated hairless mice was examined.					
35294989	0	62	theme	UVB-exposed	100:110	arg1	Mice					121:124	UVB-exposed Hairless Mice	100:124	UVB-exposed Hairless Mice	100:124	Effects of Collagen-Tripeptide and Galacto-oligosaccharide Mixture on Skin Photoaging Inhibition in UVB-exposed Hairless Mice.					
35294989	6	63	theme	acid	1046:1049	arg1	content					1058:1064	The cecal short-chain fatty acid (SCFA) content	1018:1064	The cecal short-chain fatty acid (SCFA) content	1018:1064	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	8	64	theme	cytokine	1543:1550	arg1	levels					1552:1557	cytokine levels	1543:1557	cytokine levels	1543:1557	Taken together, CTP/GOS mixtures showed a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition.					
35294989	7	65	theme	microbiota	1213:1222	arg1	analysis					1195:1202	the 16S rRNA-based analysis	1176:1202	the 16S rRNA-based analysis of cecal microbiota	1176:1222	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	5	66	theme	mixture	1002:1008	arg1	groups					1010:1015	other mixture groups	996:1015	other mixture groups	996:1015	Administration of the 1:3 mixture showed significant changes in the extracellular matrix-related gene expression compared to other mixture groups.					
35294989	6	67	theme	short-chain	1028:1038	arg1	SCFA					1052:1055	SCFA	1052:1055	SCFA	1052:1055	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	6	67	theme	short-chain	1028:1038	arg1	acid					1046:1049	short-chain fatty acid	1028:1049	The cecal short-chain fatty acid (SCFA) content	1018:1064	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	4	68	theme	photoaged	753:761	arg1	parameters					772:781	photoaged physical parameters	753:781	photoaged physical parameters	753:781	Oral administration of CTP/GOS mixtures ameliorated photoaged physical parameters and serum levels of pro-inflammatory cytokines compared to UV-irradiated control group.					
35294989	8	69	from	changes	1511:1517	arg1	composition					1585:1595	intestinal microbiota composition	1563:1595	intestinal microbiota composition	1563:1595	Taken together, CTP/GOS mixtures showed a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition.					
35294989	8	69	from	changes	1511:1517	arg1	expression					1531:1540	the gene expression	1522:1540	the gene expression	1522:1540	Taken together, CTP/GOS mixtures showed a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition.					
35294989	8	69	from	changes	1511:1517	arg1	levels					1552:1557	cytokine levels	1543:1557	cytokine levels	1543:1557	Taken together, CTP/GOS mixtures showed a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition.					
35294989	6	70	theme	mixed	1111:1115	arg1	group					1117:1121	the CTP/GOS mixed group	1099:1121	the CTP/GOS mixed group	1099:1121	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	8	71	theme	microbiota	1574:1583	arg1	composition					1585:1595	intestinal microbiota composition	1563:1595	intestinal microbiota composition	1563:1595	Taken together, CTP/GOS mixtures showed a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels and intestinal microbiota composition.					
35294989	7	72	theme	rRNA-based	1184:1193	arg1	analysis					1195:1202	the 16S rRNA-based analysis	1176:1202	the 16S rRNA-based analysis of cecal microbiota	1176:1222	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	7	73	theme	CTP	1333:1335	arg1	groups					1366:1371	CTP and GOS mixture-administered groups	1333:1371	CTP and GOS mixture-administered groups	1333:1371	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	2	74	theme	photoprotective	368:382	arg1	effect					384:389	the photoprotective effect	364:389	the photoprotective effect of CTP/GOS mixtures (3:1, 1:1, and 1:3) in ultraviolet (UV) B-irradiated hairless mice	364:476	In this study, the photoprotective effect of CTP/GOS mixtures (3:1, 1:1, and 1:3) in ultraviolet (UV) B-irradiated hairless mice was examined.					
35294989	1	75	theme	food	280:283	arg1	industry					285:292	the food industry	276:292	the food industry	276:292	Collagen-tripeptide (CTP) and galacto-oligosaccharide (GOS), which improve collagen homeostasis and barrier function in the skin, are widely used in the food industry to improve wrinkle-related parameters and skin health.					
35294989	3	76	theme	photoaging	621:630	arg1	effect					643:648	the photoaging protective effect	617:648	the photoaging protective effect	617:648	Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota.					
35294989	4	77	theme	mixtures	732:739	arg1	administration					706:719	Oral administration	701:719	Oral administration of CTP/GOS mixtures	701:739	Oral administration of CTP/GOS mixtures ameliorated photoaged physical parameters and serum levels of pro-inflammatory cytokines compared to UV-irradiated control group.					
35294989	6	78	theme	higher	1130:1135	arg1	content					1141:1147	a higher GOS content	1128:1147	a higher GOS content than the control group	1128:1170	The cecal short-chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group.					
35294989	0	79	theme	Mixture	59:65	arg1	Effects					0:6	Effects	0:6	Effects of Collagen-Tripeptide and Galacto-oligosaccharide Mixture on Skin Photoaging Inhibition in UVB-exposed Hairless Mice.	0:125	Effects of Collagen-Tripeptide and Galacto-oligosaccharide Mixture on Skin Photoaging Inhibition in UVB-exposed Hairless Mice.					
35294989	1	80	used	used	268:271	arg2	Collagen-tripeptide					127:145	Collagen-tripeptide	127:145	Collagen-tripeptide (CTP)	127:151	Collagen-tripeptide (CTP) and galacto-oligosaccharide (GOS), which improve collagen homeostasis and barrier function in the skin, are widely used in the food industry to improve wrinkle-related parameters and skin health.					
35294989	1	80	used	used	268:271	arg2	GOS					182:184	GOS	182:184	GOS	182:184	Collagen-tripeptide (CTP) and galacto-oligosaccharide (GOS), which improve collagen homeostasis and barrier function in the skin, are widely used in the food industry to improve wrinkle-related parameters and skin health.					
35294989	1	80	used	used	268:271	arg2	galacto-oligosaccharide					157:179	galacto-oligosaccharide	157:179	galacto-oligosaccharide (GOS)	157:185	Collagen-tripeptide (CTP) and galacto-oligosaccharide (GOS), which improve collagen homeostasis and barrier function in the skin, are widely used in the food industry to improve wrinkle-related parameters and skin health.					
35294989	1	80	used	used	268:271	arg2	CTP					148:150	CTP	148:150	CTP	148:150	Collagen-tripeptide (CTP) and galacto-oligosaccharide (GOS), which improve collagen homeostasis and barrier function in the skin, are widely used in the food industry to improve wrinkle-related parameters and skin health.					
35294989	7	81	theme	GOS	1341:1343	arg1	groups					1366:1371	CTP and GOS mixture-administered groups	1333:1371	CTP and GOS mixture-administered groups	1333:1371	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	2	82	dep	mixtures	402:409	arg1	1:1					417:419	1:1	417:419	1:1	417:419	In this study, the photoprotective effect of CTP/GOS mixtures (3:1, 1:1, and 1:3) in ultraviolet (UV) B-irradiated hairless mice was examined.					
35294989	2	82	dep	mixtures	402:409	arg1	1:3					426:428	1:3	426:428	1:3	426:428	In this study, the photoprotective effect of CTP/GOS mixtures (3:1, 1:1, and 1:3) in ultraviolet (UV) B-irradiated hairless mice was examined.					
35294989	2	82	dep	mixtures	402:409	arg1	3:1					412:414	3:1	412:414	3:1	412:414	In this study, the photoprotective effect of CTP/GOS mixtures (3:1, 1:1, and 1:3) in ultraviolet (UV) B-irradiated hairless mice was examined.					
35294989	2	83	theme	mixtures	402:409	arg1	effect					384:389	the photoprotective effect	364:389	the photoprotective effect of CTP/GOS mixtures (3:1, 1:1, and 1:3) in ultraviolet (UV) B-irradiated hairless mice	364:476	In this study, the photoprotective effect of CTP/GOS mixtures (3:1, 1:1, and 1:3) in ultraviolet (UV) B-irradiated hairless mice was examined.					
35294989	0	84	theme	Skin	70:73	arg1	Inhibition					86:95	Skin Photoaging Inhibition	70:95	Skin Photoaging Inhibition in UVB-exposed Hairless Mice	70:124	Effects of Collagen-Tripeptide and Galacto-oligosaccharide Mixture on Skin Photoaging Inhibition in UVB-exposed Hairless Mice.					
35294989	1	85	theme	wrinkle-related	305:319	arg1	parameters					321:330	wrinkle-related parameters	305:330	wrinkle-related parameters	305:330	Collagen-tripeptide (CTP) and galacto-oligosaccharide (GOS), which improve collagen homeostasis and barrier function in the skin, are widely used in the food industry to improve wrinkle-related parameters and skin health.					
35294989	3	86	theme	signaling	651:659	arg1	pathways					661:668	signaling pathways	651:668	signaling pathways	651:668	Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota.					
35294989	4	87	theme	serum	787:791	arg1	levels					793:798	serum levels	787:798	serum levels	787:798	Oral administration of CTP/GOS mixtures ameliorated photoaged physical parameters and serum levels of pro-inflammatory cytokines compared to UV-irradiated control group.					
35294989	7	88	theme	Akkermansia	1261:1271	arg1	genus					1273:1277	the Akkermansia genus	1257:1277	the Akkermansia genus belonging to the Verrucomicrobia phylum	1257:1317	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
35294989	7	89	theme	mixture-administered	1345:1364	arg1	groups					1366:1371	CTP and GOS mixture-administered groups	1333:1371	CTP and GOS mixture-administered groups	1333:1371	In the 16S rRNA-based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture-administered groups than in the UV-irradiated control group.					
36219265	0	0	theme	cartilage	103:111	arg1	defect					113:118	articular cartilage defect	93:118	articular cartilage defect	93:118	Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect.					
36219265	3	1	theme	manganese	355:363	arg1	scaffold					400:407	a manganese ion-incorporated natupolymer-based scaffold	353:407	a manganese ion-incorporated natupolymer-based scaffold with chitosan-gelatin	353:429	We prepared a manganese ion-incorporated natupolymer-based scaffold with chitosan-gelatin by freeze-drying procedure.					
36219265	5	2	theme	articular	768:776	arg1	chondrocytes					778:789	rat articular chondrocytes	764:789	rat articular chondrocytes	764:789	Live/dead assay confirmed the good cytocompatibility of prepared scaffold on rat articular chondrocytes after 10 days and 4 weeks of culture.					
36219265	6	3	theme	manganese-loaded	833:848	arg1	scaffold					860:867	The manganese-loaded composite scaffold	829:867	The manganese-loaded composite scaffold	829:867	The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes.					
36219265	0	4	theme	articular	93:101	arg1	cartilage					103:111	articular cartilage	93:111	articular cartilage defect	93:118	Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect.					
36219265	4	5	theme	compressive	624:634	arg1	testing					636:642	compressive testing	624:642	compressive testing	624:642	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	8	6	theme	composite	1185:1193	arg1	scaffold					1195:1202	current composite scaffold	1177:1202	current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model	1177:1271	Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair.					
36219265	5	7	theme	good	717:720	arg1	cytocompatibility					722:738	the good cytocompatibility	713:738	the good cytocompatibility of prepared scaffold on rat articular chondrocytes	713:789	Live/dead assay confirmed the good cytocompatibility of prepared scaffold on rat articular chondrocytes after 10 days and 4 weeks of culture.					
36219265	7	8	theme	proteins	999:1006	arg1	analysis					987:994	Western blot analysis	974:994	Western blot analysis of proteins extracted from chondrocytes grown on scaffolds	974:1053	Western blot analysis of proteins extracted from chondrocytes grown on scaffolds indicated the signaling pathways of p-Akt and p-ERK1/2 played a key role.					
36219265	3	9	theme	freeze-drying	434:446	arg1	procedure					448:456	freeze-drying procedure	434:456	freeze-drying procedure	434:456	We prepared a manganese ion-incorporated natupolymer-based scaffold with chitosan-gelatin by freeze-drying procedure.					
36219265	9	10	theme	promising	1468:1476	arg1	strategy					1478:1485	a promising strategy	1466:1485	a promising strategy for articular cartilage engineering application	1466:1533	These findings suggested the potential of manganese-loaded scaffold to promote new cartilage formation and a promising strategy for articular cartilage engineering application.					
36219265	8	11	theme	current	1177:1183	arg1	scaffold					1195:1202	current composite scaffold	1177:1202	current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model	1177:1271	Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair.					
36219265	2	12	theme	cartilage	323:331	arg1	repair					333:338	cartilage repair	323:338	cartilage repair	323:338	Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair.					
36219265	7	13	theme	blot	982:985	arg1	analysis					987:994	Western blot analysis	974:994	Western blot analysis of proteins extracted from chondrocytes grown on scaffolds	974:1053	Western blot analysis of proteins extracted from chondrocytes grown on scaffolds indicated the signaling pathways of p-Akt and p-ERK1/2 played a key role.					
36219265	8	14	theme	collagen	1325:1332	arg1	formation					1312:1320	the formation	1308:1320	the formation of collagen II and cartilage repair	1308:1356	Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair.					
36219265	4	15	theme	electron	571:578	arg1	microscopy					580:589	scanning electron microscopy	562:589	scanning electron microscopy	562:589	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	5	16	theme	prepared	743:750	arg1	scaffold					752:759	prepared scaffold	743:759	prepared scaffold	743:759	Live/dead assay confirmed the good cytocompatibility of prepared scaffold on rat articular chondrocytes after 10 days and 4 weeks of culture.					
36219265	7	17	theme	p-Akt	1091:1095	arg1	pathways					1079:1086	the signaling pathways	1065:1086	the signaling pathways of p-Akt and p-ERK1/2	1065:1108	Western blot analysis of proteins extracted from chondrocytes grown on scaffolds indicated the signaling pathways of p-Akt and p-ERK1/2 played a key role.					
36219265	6	18	dep	markers	920:926	arg1	Sox9					929:932	Sox9	929:932	Sox9	929:932	The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes.					
36219265	6	18	dep	markers	920:926	arg1	markers					920:926	chondrogenic-related markers	899:926	chondrogenic-related markers (Sox9, integrin, and Col II)	899:955	The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes.					
36219265	6	18	dep	markers	920:926	arg1	integrin					935:942	integrin	935:942	integrin	935:942	The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes.					
36219265	6	18	dep	markers	920:926	arg1	Col					949:951	Col	949:951	Col	949:951	The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes.					
36219265	4	19	theme	energy	592:597	arg1	spectroscopy					610:621	energy dispersive spectroscopy	592:621	energy dispersive spectroscopy	592:621	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	4	20	theme	dispersive	599:608	arg1	spectroscopy					610:621	energy dispersive spectroscopy	592:621	energy dispersive spectroscopy	592:621	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	7	21	theme	signaling	1069:1077	arg1	pathways					1079:1086	the signaling pathways	1065:1086	the signaling pathways of p-Akt and p-ERK1/2	1065:1108	Western blot analysis of proteins extracted from chondrocytes grown on scaffolds indicated the signaling pathways of p-Akt and p-ERK1/2 played a key role.					
36219265	2	22	theme	strategy	310:317	arg1	development					291:301	development	291:301	development	291:301	Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair.					
36219265	2	22	theme	strategy	310:317	arg1	investigation					273:285	investigation	273:285	investigation	273:285	Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair.					
36219265	3	23	theme	ion-incorporated	365:380	arg1	scaffold					400:407	a manganese ion-incorporated natupolymer-based scaffold	353:407	a manganese ion-incorporated natupolymer-based scaffold with chitosan-gelatin	353:429	We prepared a manganese ion-incorporated natupolymer-based scaffold with chitosan-gelatin by freeze-drying procedure.					
36219265	5	24	theme	Live/dead	687:695	arg1	assay					697:701	Live/dead assay	687:701	Live/dead assay	687:701	Live/dead assay confirmed the good cytocompatibility of prepared scaffold on rat articular chondrocytes after 10 days and 4 weeks of culture.					
36219265	7	25	theme	Western	974:980	arg1	analysis					987:994	Western blot analysis	974:994	Western blot analysis of proteins extracted from chondrocytes grown on scaffolds	974:1053	Western blot analysis of proteins extracted from chondrocytes grown on scaffolds indicated the signaling pathways of p-Akt and p-ERK1/2 played a key role.					
36219265	0	26	theme	Chondrogenic	0:11	arg1	potential					13:21	Chondrogenic potential	0:21	Chondrogenic potential of manganese-loaded composite scaffold	0:60	Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect.					
36219265	4	27	theme	porosity	661:668	arg1	spectroscopy					610:621	energy dispersive spectroscopy	592:621	energy dispersive spectroscopy	592:621	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	4	27	theme	porosity	661:668	arg1	spectroscopy					520:531	spectroscopy	520:531	spectroscopy	520:531	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	4	27	theme	porosity	661:668	arg1	microscopy					580:589	scanning electron microscopy	562:589	scanning electron microscopy	562:589	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	4	27	theme	porosity	661:668	arg1	testing					636:642	compressive testing	624:642	compressive testing	624:642	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	4	27	theme	porosity	661:668	arg1	analysis					552:559	thermogravimetric analysis	534:559	thermogravimetric analysis	534:559	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	4	27	theme	porosity	661:668	arg1	analysis					649:656	analysis	649:656	analysis of porosity and flexibility	649:684	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	5	28	theme	rat	764:766	arg1	chondrocytes					778:789	rat articular chondrocytes	764:789	rat articular chondrocytes	764:789	Live/dead assay confirmed the good cytocompatibility of prepared scaffold on rat articular chondrocytes after 10 days and 4 weeks of culture.					
36219265	9	29	theme	scaffold	1418:1425	arg1	potential					1388:1396	the potential	1384:1396	the potential of manganese-loaded scaffold to promote new cartilage formation and a promising strategy for articular cartilage engineering application	1384:1533	These findings suggested the potential of manganese-loaded scaffold to promote new cartilage formation and a promising strategy for articular cartilage engineering application.					
36219265	4	30	theme	thermogravimetric	534:550	arg1	analysis					552:559	thermogravimetric analysis	534:559	thermogravimetric analysis	534:559	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	0	31	theme	manganese-loaded	26:41	arg1	scaffold					53:60	manganese-loaded composite scaffold	26:60	manganese-loaded composite scaffold	26:60	Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect.					
36219265	8	32	theme	scaffold	1195:1202	arg1	implantation					1161:1172	implantation	1161:1172	implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model	1161:1271	Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair.					
36219265	6	33	theme	markers	920:926	arg1	expression					885:894	the expression	881:894	the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes	881:971	The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes.					
36219265	8	34	theme	repair	1351:1356	arg1	formation					1312:1320	the formation	1308:1320	the formation of collagen II and cartilage repair	1308:1356	Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair.					
36219265	8	35	theme	cartilage	1341:1349	arg1	repair					1351:1356	cartilage repair	1341:1356	cartilage repair	1341:1356	Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair.					
36219265	4	36	theme	flexibility	674:684	arg1	spectroscopy					610:621	energy dispersive spectroscopy	592:621	energy dispersive spectroscopy	592:621	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	4	36	theme	flexibility	674:684	arg1	spectroscopy					520:531	spectroscopy	520:531	spectroscopy	520:531	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	4	36	theme	flexibility	674:684	arg1	microscopy					580:589	scanning electron microscopy	562:589	scanning electron microscopy	562:589	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	4	36	theme	flexibility	674:684	arg1	testing					636:642	compressive testing	624:642	compressive testing	624:642	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	4	36	theme	flexibility	674:684	arg1	analysis					552:559	thermogravimetric analysis	534:559	thermogravimetric analysis	534:559	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	4	36	theme	flexibility	674:684	arg1	analysis					649:656	analysis	649:656	analysis of porosity and flexibility	649:684	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	5	37	theme	culture	820:826	arg1	days					800:803	10 days	797:803	10 days	797:803	Live/dead assay confirmed the good cytocompatibility of prepared scaffold on rat articular chondrocytes after 10 days and 4 weeks of culture.					
36219265	5	37	theme	culture	820:826	arg1	weeks					811:815	4 weeks	809:815	4 weeks	809:815	Live/dead assay confirmed the good cytocompatibility of prepared scaffold on rat articular chondrocytes after 10 days and 4 weeks of culture.					
36219265	2	38	theme	regenerative	204:215	arg1	capacity					217:224	potential regenerative capacity	194:224	potential regenerative capacity to reconstruct chondral defect	194:255	Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair.					
36219265	0	39	theme	scaffold	53:60	arg1	potential					13:21	Chondrogenic potential	0:21	Chondrogenic potential of manganese-loaded composite scaffold	0:60	Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect.					
36219265	8	40	theme	articular	1240:1248	arg1	model					1267:1271	a rat articular cartilage defect model	1234:1271	a rat articular cartilage defect model	1234:1271	Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair.					
36219265	2	41	theme	potential	194:202	arg1	capacity					217:224	potential regenerative capacity	194:224	potential regenerative capacity to reconstruct chondral defect	194:255	Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair.					
36219265	0	42	theme	composite	43:51	arg1	scaffold					53:60	manganese-loaded composite scaffold	26:60	manganese-loaded composite scaffold	26:60	Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect.					
36219265	8	43	theme	rat	1236:1238	arg1	model					1267:1271	a rat articular cartilage defect model	1234:1271	a rat articular cartilage defect model	1234:1271	Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair.					
36219265	4	44	theme	scanning	562:569	arg1	microscopy					580:589	scanning electron microscopy	562:589	scanning electron microscopy	562:589	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	2	45	theme	accelerated	261:271	arg1	investigation					273:285	investigation	273:285	investigation	273:285	Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair.					
36219265	6	46	theme	chondrogenic-related	899:918	arg1	Sox9					929:932	Sox9	929:932	Sox9	929:932	The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes.					
36219265	6	46	theme	chondrogenic-related	899:918	arg1	markers					920:926	chondrogenic-related markers	899:926	chondrogenic-related markers (Sox9, integrin, and Col II)	899:955	The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes.					
36219265	6	46	theme	chondrogenic-related	899:918	arg1	integrin					935:942	integrin	935:942	integrin	935:942	The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes.					
36219265	6	46	theme	chondrogenic-related	899:918	arg1	Col					949:951	Col	949:951	Col	949:951	The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes.					
36219265	9	47	theme	new	1438:1440	arg1	formation					1452:1460	new cartilage formation	1438:1460	new cartilage formation	1438:1460	These findings suggested the potential of manganese-loaded scaffold to promote new cartilage formation and a promising strategy for articular cartilage engineering application.					
36219265	8	48	theme	defect	1260:1265	arg1	model					1267:1271	a rat articular cartilage defect model	1234:1271	a rat articular cartilage defect model	1234:1271	Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair.					
36219265	9	49	theme	manganese-loaded	1401:1416	arg1	scaffold					1418:1425	manganese-loaded scaffold	1401:1425	manganese-loaded scaffold	1401:1425	These findings suggested the potential of manganese-loaded scaffold to promote new cartilage formation and a promising strategy for articular cartilage engineering application.					
36219265	6	50	from	expression	885:894	arg1	chondrocytes					960:971	chondrocytes	960:971	chondrocytes	960:971	The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes.					
36219265	8	51	theme	cartilage	1250:1258	arg1	model					1267:1271	a rat articular cartilage defect model	1234:1271	a rat articular cartilage defect model	1234:1271	Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair.					
36219265	2	52	theme	chondral	241:248	arg1	defect					250:255	chondral defect	241:255	chondral defect	241:255	Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair.					
36219265	5	53	theme	scaffold	752:759	arg1	cytocompatibility					722:738	the good cytocompatibility	713:738	the good cytocompatibility of prepared scaffold on rat articular chondrocytes	713:789	Live/dead assay confirmed the good cytocompatibility of prepared scaffold on rat articular chondrocytes after 10 days and 4 weeks of culture.					
36219265	1	54	theme	alymphatic	137:146	arg1	Cartilage					121:129	Cartilage	121:129	Cartilage	121:129	Cartilage is an alymphatic, avascular and non-innervated tissue.					
36219265	1	54	theme	alymphatic	137:146	arg1	tissue					178:183	an alymphatic, avascular and non-innervated tissue	134:183	an alymphatic, avascular and non-innervated tissue	134:183	Cartilage is an alymphatic, avascular and non-innervated tissue.					
36219265	8	55	theme	Histological	1129:1140	arg1	analysis					1142:1149	Histological analysis	1129:1149	Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model	1129:1271	Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair.					
36219265	2	56	theme	new	306:308	arg1	strategy					310:317	new strategy	306:317	new strategy for cartilage repair	306:338	Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair.					
36219265	7	57	theme	p-ERK1/2	1101:1108	arg1	pathways					1079:1086	the signaling pathways	1065:1086	the signaling pathways of p-Akt and p-ERK1/2	1065:1108	Western blot analysis of proteins extracted from chondrocytes grown on scaffolds indicated the signaling pathways of p-Akt and p-ERK1/2 played a key role.					
36219265	7	58	theme	key	1119:1121	arg1	role					1123:1126	a key role	1117:1126	a key role	1117:1126	Western blot analysis of proteins extracted from chondrocytes grown on scaffolds indicated the signaling pathways of p-Akt and p-ERK1/2 played a key role.					
36219265	1	59	theme	avascular	149:157	arg1	Cartilage					121:129	Cartilage	121:129	Cartilage	121:129	Cartilage is an alymphatic, avascular and non-innervated tissue.					
36219265	1	59	theme	avascular	149:157	arg1	tissue					178:183	an alymphatic, avascular and non-innervated tissue	134:183	an alymphatic, avascular and non-innervated tissue	134:183	Cartilage is an alymphatic, avascular and non-innervated tissue.					
36219265	2	60	contain	has	257:259	arg1	Lack					186:189	Lack	186:189	Lack of potential regenerative capacity to reconstruct chondral defect	186:255	Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair.					
36219265	2	60	contain	has	257:259	arg2	investigation					273:285	investigation	273:285	investigation	273:285	Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair.					
36219265	2	60	contain	has	257:259	arg2	development					291:301	development	291:301	development	291:301	Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair.					
36219265	3	61	theme	natupolymer-based	382:398	arg1	scaffold					400:407	a manganese ion-incorporated natupolymer-based scaffold	353:407	a manganese ion-incorporated natupolymer-based scaffold with chitosan-gelatin	353:429	We prepared a manganese ion-incorporated natupolymer-based scaffold with chitosan-gelatin by freeze-drying procedure.					
36219265	2	62	theme	capacity	217:224	arg1	Lack					186:189	Lack	186:189	Lack of potential regenerative capacity to reconstruct chondral defect	186:255	Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair.					
36219265	9	63	theme	articular	1491:1499	arg1	cartilage					1501:1509	articular cartilage	1491:1509	articular cartilage engineering application	1491:1533	These findings suggested the potential of manganese-loaded scaffold to promote new cartilage formation and a promising strategy for articular cartilage engineering application.					
36219265	7	64	dep	indicated	1055:1063	arg1	played					1110:1115	played	1110:1115	indicated the signaling pathways of p-Akt and p-ERK1/2 played a key role	1055:1126	Western blot analysis of proteins extracted from chondrocytes grown on scaffolds indicated the signaling pathways of p-Akt and p-ERK1/2 played a key role.					
36219265	5	65	from	cytocompatibility	722:738	arg1	chondrocytes					778:789	rat articular chondrocytes	764:789	rat articular chondrocytes	764:789	Live/dead assay confirmed the good cytocompatibility of prepared scaffold on rat articular chondrocytes after 10 days and 4 weeks of culture.					
36219265	9	66	theme	cartilage	1442:1450	arg1	formation					1452:1460	new cartilage formation	1438:1460	new cartilage formation	1438:1460	These findings suggested the potential of manganese-loaded scaffold to promote new cartilage formation and a promising strategy for articular cartilage engineering application.					
36219265	3	67	with	scaffold	400:407	arg1	chitosan-gelatin					414:429	chitosan-gelatin	414:429	chitosan-gelatin	414:429	We prepared a manganese ion-incorporated natupolymer-based scaffold with chitosan-gelatin by freeze-drying procedure.					
36219265	6	68	theme	composite	850:858	arg1	scaffold					860:867	The manganese-loaded composite scaffold	829:867	The manganese-loaded composite scaffold	829:867	The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes.					
36219265	1	69	theme	non-innervated	163:176	arg1	Cartilage					121:129	Cartilage	121:129	Cartilage	121:129	Cartilage is an alymphatic, avascular and non-innervated tissue.					
36219265	1	69	theme	non-innervated	163:176	arg1	tissue					178:183	an alymphatic, avascular and non-innervated tissue	134:183	an alymphatic, avascular and non-innervated tissue	134:183	Cartilage is an alymphatic, avascular and non-innervated tissue.					
36219265	4	70	dep	Fourier	493:499	arg1	transform					501:509	transform	501:509	transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility	501:684	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
36219265	4	71	dep	transform	501:509	arg1	infrared					511:518	infrared	511:518	transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility	501:684	The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility.					
37016048	0	0	theme	THP-1	91:95	arg1	monocytes					97:105	human THP-1 monocytes	85:105	human THP-1 monocytes	85:105	Dietary fatty acids differentially affect secretion of pro-inflammatory cytokines in human THP-1 monocytes.					
37016048	2	1	theme	acids	355:359	arg1	concentrations					323:336	concentrations	323:336	concentrations	323:336	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	2	1	theme	acids	355:359	arg1	combinations					306:317	combinations	306:317	combinations	306:317	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	9	2	theme	FAs	1410:1412	arg1	compositions					1367:1378	the chemical compositions	1354:1378	the chemical compositions	1354:1378	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	9	2	theme	FAs	1410:1412	arg1	factors					1422:1428	key factors	1418:1428	key factors in the intricate regulation of monocyte-mediated inflammation	1418:1490	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	9	2	theme	FAs	1410:1412	arg1	concentrations					1384:1397	concentrations	1384:1397	concentrations of dietary FAs	1384:1412	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	0	3	theme	human	85:89	arg1	monocytes					97:105	human THP-1 monocytes	85:105	human THP-1 monocytes	85:105	Dietary fatty acids differentially affect secretion of pro-inflammatory cytokines in human THP-1 monocytes.					
37016048	2	4	from	concentrations	323:336	arg1	blood					370:374	blood	370:374	blood	370:374	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	2	4	from	concentrations	323:336	arg1	fats					388:391	dietary fats	380:391	dietary fats	380:391	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	2	5	theme	fatty	349:353	arg1	acids					355:359	certain fatty acids	341:359	certain fatty acids (FAs) in blood and dietary fats	341:391	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	2	5	theme	fatty	349:353	arg1	FAs					362:364	FAs	362:364	FAs	362:364	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	2	6	from	combinations	306:317	arg1	blood					370:374	blood	370:374	blood	370:374	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	2	6	from	combinations	306:317	arg1	fats					388:391	dietary fats	380:391	dietary fats	380:391	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	7	7	dep	U-shaped	1056:1063	arg1	curves					1123:1128	dose-response curves	1109:1128	dose-response curves	1109:1128	Interestingly, the inhibitory potentials of MUFAs and PUFAs followed U-shaped (TNF and IL-1β) or inverted U-shaped (IL-6) dose-response curves.					
37016048	7	7	dep	U-shaped	1056:1063	arg1	IL-1β					1074:1078	IL-1β	1074:1078	IL-1β	1074:1078	Interestingly, the inhibitory potentials of MUFAs and PUFAs followed U-shaped (TNF and IL-1β) or inverted U-shaped (IL-6) dose-response curves.					
37016048	7	7	dep	U-shaped	1056:1063	arg1	TNF					1066:1068	TNF	1066:1068	TNF	1066:1068	Interestingly, the inhibitory potentials of MUFAs and PUFAs followed U-shaped (TNF and IL-1β) or inverted U-shaped (IL-6) dose-response curves.					
37016048	4	8	dep	absence	703:709	arg1	the					699:701	the	699:701	the	699:701	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	5	9	theme	potent	864:869	arg1	species					871:877	the most potent species	855:877	the most potent species	855:877	SFAs generally stimulated resting THP-1 cells to secrete pro-inflammatory cytokines, with stearic acid being the most potent species.					
37016048	4	10	theme	FAs	549:551	arg1	SFAs					554:557	six saturated FAs (SFAs)	535:558	six saturated FAs (SFAs)	535:558	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	9	11	theme	inflammation	1479:1490	arg1	regulation					1447:1456	the intricate regulation	1433:1456	the intricate regulation of monocyte-mediated inflammation	1433:1490	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	3	12	from	effects	431:437	arg1	secretion					458:466	cytokine secretion	449:466	cytokine secretion by monocytes	449:479	However, systemic comparisons of the effects of FAs on cytokine secretion by monocytes have not be performed.					
37016048	1	13	theme	cells	163:167	arg1	population					130:139	a major population	122:139	a major population of circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body	122:244	Monocytes are a major population of circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body.					
37016048	1	13	theme	cells	163:167	arg1	Monocytes					108:116	Monocytes	108:116	Monocytes	108:116	Monocytes are a major population of circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body.					
37016048	9	14	theme	chemical	1358:1365	arg1	compositions					1367:1378	the chemical compositions	1354:1378	the chemical compositions	1354:1378	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	9	14	theme	chemical	1358:1365	arg1	concentrations					1384:1397	concentrations	1384:1397	concentrations of dietary FAs	1384:1412	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	9	14	theme	chemical	1358:1365	arg1	factors					1422:1428	key factors	1418:1428	key factors in the intricate regulation of monocyte-mediated inflammation	1418:1490	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	4	15	theme	saturated	539:547	arg1	SFAs					554:557	six saturated FAs (SFAs)	535:558	six saturated FAs (SFAs)	535:558	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	8	16	theme	anti-inflammatory	1291:1307	arg1	compound					1309:1316	the most potent anti-inflammatory compound	1275:1316	the most potent anti-inflammatory compound	1275:1316	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	8	16	theme	anti-inflammatory	1291:1307	arg1	acid					1193:1196	docosahexaenoic acid	1177:1196	docosahexaenoic acid (C22:6 n-3)	1177:1208	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	9	17	from	factors	1422:1428	arg1	regulation					1447:1456	the intricate regulation	1433:1456	the intricate regulation of monocyte-mediated inflammation	1433:1490	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	5	18	theme	THP-1	780:784	arg1	cells					786:790	resting THP-1 cells	772:790	resting THP-1 cells	772:790	SFAs generally stimulated resting THP-1 cells to secrete pro-inflammatory cytokines, with stearic acid being the most potent species.					
37016048	7	19	theme	dose-response	1109:1121	arg1	curves					1123:1128	dose-response curves	1109:1128	dose-response curves	1109:1128	Interestingly, the inhibitory potentials of MUFAs and PUFAs followed U-shaped (TNF and IL-1β) or inverted U-shaped (IL-6) dose-response curves.					
37016048	0	20	from	secretion	42:50	arg1	monocytes					97:105	human THP-1 monocytes	85:105	human THP-1 monocytes	85:105	Dietary fatty acids differentially affect secretion of pro-inflammatory cytokines in human THP-1 monocytes.					
37016048	4	21	theme	lipopolysaccharide	726:743	arg1	presence					714:721	presence	714:721	presence	714:721	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	4	21	theme	lipopolysaccharide	726:743	arg1	absence					703:709	absence	703:709	absence	703:709	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	2	22	from	acids	355:359	arg1	blood					370:374	blood	370:374	blood	370:374	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	2	22	from	acids	355:359	arg1	fats					388:391	dietary fats	380:391	dietary fats	380:391	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	4	23	theme	IL-1β	680:684	arg1	secretion					662:670	human THP-1 monocyte secretion	641:670	human THP-1 monocyte secretion of TNF, IL-1β, and IL-6	641:694	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	5	24	theme	resting	772:778	arg1	cells					786:790	resting THP-1 cells	772:790	resting THP-1 cells	772:790	SFAs generally stimulated resting THP-1 cells to secrete pro-inflammatory cytokines, with stearic acid being the most potent species.					
37016048	0	25	theme	fatty	8:12	arg1	acids					14:18	Dietary fatty acids	0:18	Dietary fatty acids	0:18	Dietary fatty acids differentially affect secretion of pro-inflammatory cytokines in human THP-1 monocytes.					
37016048	9	26	theme	monocyte-mediated	1461:1477	arg1	inflammation					1479:1490	monocyte-mediated inflammation	1461:1490	monocyte-mediated inflammation	1461:1490	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	4	27	theme	monocyte	653:660	arg1	secretion					662:670	human THP-1 monocyte secretion	641:670	human THP-1 monocyte secretion of TNF, IL-1β, and IL-6	641:694	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	1	28	theme	crucial	181:187	arg1	role					189:192	a crucial role	179:192	a crucial role	179:192	Monocytes are a major population of circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body.					
37016048	0	29	theme	Dietary	0:6	arg1	acids					14:18	Dietary fatty acids	0:18	Dietary fatty acids	0:18	Dietary fatty acids differentially affect secretion of pro-inflammatory cytokines in human THP-1 monocytes.					
37016048	8	30	theme	largest	1224:1230	arg1	number					1232:1237	the largest number	1220:1237	the largest number of double bonds	1220:1253	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	7	31	theme	MUFAs	1031:1035	arg1	potentials					1017:1026	the inhibitory potentials	1002:1026	the inhibitory potentials of MUFAs and PUFAs	1002:1045	Interestingly, the inhibitory potentials of MUFAs and PUFAs followed U-shaped (TNF and IL-1β) or inverted U-shaped (IL-6) dose-response curves.					
37016048	3	32	from	secretion	458:466	arg1	comparisons					412:422	systemic comparisons	403:422	systemic comparisons of the effects of FAs on cytokine secretion by monocytes	403:479	However, systemic comparisons of the effects of FAs on cytokine secretion by monocytes have not be performed.					
37016048	4	33	theme	THP-1	647:651	arg1	secretion					662:670	human THP-1 monocyte secretion	641:670	human THP-1 monocyte secretion of TNF, IL-1β, and IL-6	641:694	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	3	34	theme	systemic	403:410	arg1	comparisons					412:422	systemic comparisons	403:422	systemic comparisons of the effects of FAs on cytokine secretion by monocytes	403:479	However, systemic comparisons of the effects of FAs on cytokine secretion by monocytes have not be performed.					
37016048	7	35	theme	inhibitory	1006:1015	arg1	potentials					1017:1026	the inhibitory potentials	1002:1026	the inhibitory potentials of MUFAs and PUFAs	1002:1045	Interestingly, the inhibitory potentials of MUFAs and PUFAs followed U-shaped (TNF and IL-1β) or inverted U-shaped (IL-6) dose-response curves.					
37016048	5	36	theme	pro-inflammatory	803:818	arg1	cytokines					820:828	pro-inflammatory cytokines	803:828	pro-inflammatory cytokines	803:828	SFAs generally stimulated resting THP-1 cells to secrete pro-inflammatory cytokines, with stearic acid being the most potent species.					
37016048	8	37	theme	potent	1284:1289	arg1	compound					1309:1316	the most potent anti-inflammatory compound	1275:1316	the most potent anti-inflammatory compound	1275:1316	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	8	37	theme	potent	1284:1289	arg1	acid					1193:1196	docosahexaenoic acid	1177:1196	docosahexaenoic acid (C22:6 n-3)	1177:1208	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	4	38	theme	monounsaturated	565:579	arg1	MUFAs					586:590	two monounsaturated FAs (MUFAs)	561:591	two monounsaturated FAs (MUFAs)	561:591	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	8	39	dep	MUFAs	1141:1145	arg1	the					1137:1139	the	1137:1139	the	1137:1139	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	4	40	theme	FAs	620:622	arg1	PUFAs					625:629	seven polyunsaturated FAs (PUFAs)	598:630	seven polyunsaturated FAs (PUFAs)	598:630	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	3	41	theme	FAs	442:444	arg1	effects					431:437	the effects	427:437	the effects of FAs on cytokine secretion by monocytes	427:479	However, systemic comparisons of the effects of FAs on cytokine secretion by monocytes have not be performed.					
37016048	8	42	theme	C22:6	1199:1203	arg1	acid					1193:1196	docosahexaenoic acid	1177:1196	docosahexaenoic acid (C22:6 n-3)	1177:1208	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	8	42	theme	C22:6	1199:1203	arg1	n-3					1205:1207	C22:6 n-3	1199:1207	C22:6 n-3	1199:1207	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	9	43	theme	dietary	1402:1408	arg1	FAs					1410:1412	dietary FAs	1402:1412	dietary FAs	1402:1412	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	6	44	theme	cytokines	976:984	arg1	secretion					946:954	lipopolysaccharide-induced secretion	919:954	lipopolysaccharide-induced secretion of pro-inflammatory cytokines	919:984	In contrast, MUFAs and PUFAs inhibited lipopolysaccharide-induced secretion of pro-inflammatory cytokines.					
37016048	4	45	theme	FAs	581:583	arg1	MUFAs					586:590	two monounsaturated FAs (MUFAs)	561:591	two monounsaturated FAs (MUFAs)	561:591	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	1	46	theme	pro-inflammatory	207:222	arg1	cytokines					224:232	pro-inflammatory cytokines	207:232	pro-inflammatory cytokines in the body	207:244	Monocytes are a major population of circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body.					
37016048	4	47	theme	polyunsaturated	604:618	arg1	PUFAs					625:629	seven polyunsaturated FAs (PUFAs)	598:630	seven polyunsaturated FAs (PUFAs)	598:630	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	4	48	theme	TNF	675:677	arg1	secretion					662:670	human THP-1 monocyte secretion	641:670	human THP-1 monocyte secretion of TNF, IL-1β, and IL-6	641:694	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	2	49	theme	dietary	380:386	arg1	fats					388:391	dietary fats	380:391	dietary fats	380:391	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	0	50	theme	pro-inflammatory	55:70	arg1	cytokines					72:80	pro-inflammatory cytokines	55:80	pro-inflammatory cytokines	55:80	Dietary fatty acids differentially affect secretion of pro-inflammatory cytokines in human THP-1 monocytes.					
37016048	6	51	theme	pro-inflammatory	959:974	arg1	cytokines					976:984	pro-inflammatory cytokines	959:984	pro-inflammatory cytokines	959:984	In contrast, MUFAs and PUFAs inhibited lipopolysaccharide-induced secretion of pro-inflammatory cytokines.					
37016048	1	52	from	cytokines	224:232	arg1	body					241:244	the body	237:244	the body	237:244	Monocytes are a major population of circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body.					
37016048	2	53	theme	certain	341:347	arg1	acids					355:359	certain fatty acids	341:359	certain fatty acids (FAs) in blood and dietary fats	341:391	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	2	53	theme	certain	341:347	arg1	FAs					362:364	FAs	362:364	FAs	362:364	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	9	54	theme	key	1418:1420	arg1	factors					1422:1428	key factors	1418:1428	key factors in the intricate regulation of monocyte-mediated inflammation	1418:1490	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	9	54	theme	key	1418:1420	arg1	concentrations					1384:1397	concentrations	1384:1397	concentrations of dietary FAs	1384:1412	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	9	54	theme	key	1418:1420	arg1	compositions					1367:1378	the chemical compositions	1354:1378	the chemical compositions	1354:1378	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	9	55	theme	intricate	1437:1445	arg1	regulation					1447:1456	the intricate regulation	1433:1456	the intricate regulation of monocyte-mediated inflammation	1433:1490	Together, our findings reveal that the chemical compositions and concentrations of dietary FAs are key factors in the intricate regulation of monocyte-mediated inflammation.					
37016048	7	56	theme	PUFAs	1041:1045	arg1	potentials					1017:1026	the inhibitory potentials	1002:1026	the inhibitory potentials of MUFAs and PUFAs	1002:1045	Interestingly, the inhibitory potentials of MUFAs and PUFAs followed U-shaped (TNF and IL-1β) or inverted U-shaped (IL-6) dose-response curves.					
37016048	1	57	theme	major	124:128	arg1	population					130:139	a major population	122:139	a major population of circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body	122:244	Monocytes are a major population of circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body.					
37016048	1	57	theme	major	124:128	arg1	Monocytes					108:116	Monocytes	108:116	Monocytes	108:116	Monocytes are a major population of circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body.					
37016048	4	58	theme	IL-6	691:694	arg1	secretion					662:670	human THP-1 monocyte secretion	641:670	human THP-1 monocyte secretion of TNF, IL-1β, and IL-6	641:694	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	2	59	theme	monocytes	262:270	arg1	actions					251:257	The actions	247:257	The actions of monocytes	247:270	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	2	60	from	blood	370:374	arg1	concentrations					323:336	concentrations	323:336	concentrations	323:336	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	2	60	from	blood	370:374	arg1	combinations					306:317	combinations	306:317	combinations	306:317	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	2	61	dep	combinations	306:317	arg1	the					302:304	the	302:304	the	302:304	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	5	62	theme	stearic	836:842	arg1	acid					844:847	stearic acid	836:847	stearic acid being the most potent species	836:877	SFAs generally stimulated resting THP-1 cells to secrete pro-inflammatory cytokines, with stearic acid being the most potent species.					
37016048	8	63	theme	bonds	1249:1253	arg1	number					1232:1237	the largest number	1220:1237	the largest number of double bonds	1220:1253	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	2	64	from	fats	388:391	arg1	concentrations					323:336	concentrations	323:336	concentrations	323:336	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	2	64	from	fats	388:391	arg1	combinations					306:317	combinations	306:317	combinations	306:317	The actions of monocytes are known to be influenced by the combinations and concentrations of certain fatty acids (FAs) in blood and dietary fats.					
37016048	3	65	theme	cytokine	449:456	arg1	secretion					458:466	cytokine secretion	449:466	cytokine secretion by monocytes	449:479	However, systemic comparisons of the effects of FAs on cytokine secretion by monocytes have not be performed.					
37016048	8	66	theme	double	1242:1247	arg1	bonds					1249:1253	double bonds	1242:1253	double bonds	1242:1253	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	4	67	theme	human	641:645	arg1	secretion					662:670	human THP-1 monocyte secretion	641:670	human THP-1 monocyte secretion of TNF, IL-1β, and IL-6	641:694	In this study, we compared how six saturated FAs (SFAs), two monounsaturated FAs (MUFAs), and seven polyunsaturated FAs (PUFAs) modulate human THP-1 monocyte secretion of TNF, IL-1β, and IL-6 in the absence or presence of lipopolysaccharide.					
37016048	3	68	theme	effects	431:437	arg1	comparisons					412:422	systemic comparisons	403:422	systemic comparisons of the effects of FAs on cytokine secretion by monocytes	403:479	However, systemic comparisons of the effects of FAs on cytokine secretion by monocytes have not be performed.					
37016048	8	69	theme	docosahexaenoic	1177:1191	arg1	compound					1309:1316	the most potent anti-inflammatory compound	1275:1316	the most potent anti-inflammatory compound	1275:1316	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	8	69	theme	docosahexaenoic	1177:1191	arg1	acid					1193:1196	docosahexaenoic acid	1177:1196	docosahexaenoic acid (C22:6 n-3)	1177:1208	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	8	69	theme	docosahexaenoic	1177:1191	arg1	n-3					1205:1207	C22:6 n-3	1199:1207	C22:6 n-3	1199:1207	Among the MUFAs and PUFAs that were analyzed, docosahexaenoic acid (C22:6 n-3) exhibited the largest number of double bonds and was found to be the most potent anti-inflammatory compound.					
37016048	1	70	theme	circulating	144:154	arg1	cells					163:167	circulating immune cells	144:167	circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body	144:244	Monocytes are a major population of circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body.					
37016048	6	71	theme	lipopolysaccharide-induced	919:944	arg1	secretion					946:954	lipopolysaccharide-induced secretion	919:954	lipopolysaccharide-induced secretion of pro-inflammatory cytokines	919:984	In contrast, MUFAs and PUFAs inhibited lipopolysaccharide-induced secretion of pro-inflammatory cytokines.					
37016048	0	72	theme	cytokines	72:80	arg1	secretion					42:50	secretion	42:50	secretion of pro-inflammatory cytokines in human THP-1 monocytes	42:105	Dietary fatty acids differentially affect secretion of pro-inflammatory cytokines in human THP-1 monocytes.					
37016048	3	73	from	comparisons	412:422	arg1	secretion					458:466	cytokine secretion	449:466	cytokine secretion by monocytes	449:479	However, systemic comparisons of the effects of FAs on cytokine secretion by monocytes have not be performed.					
37016048	1	74	theme	immune	156:161	arg1	cells					163:167	circulating immune cells	144:167	circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body	144:244	Monocytes are a major population of circulating immune cells that play a crucial role in producing pro-inflammatory cytokines in the body.					
36734084	5	0	theme	pH	678:679	arg1	effect					659:664	the effect	655:664	the effect of heat and pH on the postbiotics antibacterial activity	655:721	Besides, the effect of heat and pH on the postbiotics antibacterial activity was measured via the agar-well diffusion method.					
36734084	13	1	theme	in	1674:1675	arg1	model					1692:1696	in vitro and food model	1674:1696	in vitro and food model	1674:1696	postbiotic, in particular L.plantarum, may have useful functional characteristics (possible antibacterial and antioxidant) in in vitro and food model.					
36734084	7	2	theme	chemical	944:951	arg1	composition					953:963	The postbiotics chemical composition	928:963	The postbiotics chemical composition	928:963	The postbiotics chemical composition was identified via gas chromatography-mass spectrometry.					
36734084	4	3	theme	agar-disk	618:626	arg1	method					638:643	agar-disk diffusion method	618:643	agar-disk diffusion method	618:643	Potential activity of postbiotics was performed via agar-disk diffusion method.					
36734084	11	4	theme	low	1419:1421	arg1	concentrations					1447:1460	low minimum effective( MEC) concentrations	1419:1460	a low minimum effective( MEC) concentrations index (15 mg/mL)	1417:1477	Lower minimum effective concentrations of postbiotic were altered in food model, and substantially, a low minimum effective( MEC) concentrations index (15 mg/mL) was identified for postbiotic of L.plantarum.					
36734084	6	5	theme	postbiotics	857:867	arg1	scavenging					829:838	the free radical scavenging potential	812:848	the free radical scavenging potential of the postbiotics	812:867	To determine the antioxidant effect and the free radical scavenging potential of the postbiotics, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method was utilized.					
36734084	6	5	theme	postbiotics	857:867	arg1	effect					801:806	the antioxidant effect	785:806	the antioxidant effect	785:806	To determine the antioxidant effect and the free radical scavenging potential of the postbiotics, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method was utilized.					
36734084	7	6	theme	chromatography-mass	988:1006	arg1	spectrometry					1008:1019	gas chromatography-mass spectrometry	984:1019	gas chromatography-mass spectrometry	984:1019	The postbiotics chemical composition was identified via gas chromatography-mass spectrometry.					
36734084	13	7	theme	food	1687:1690	arg1	model					1692:1696	in vitro and food model	1674:1696	in vitro and food model	1674:1696	postbiotic, in particular L.plantarum, may have useful functional characteristics (possible antibacterial and antioxidant) in in vitro and food model.					
36734084	11	8	theme	minimum	1423:1429	arg1	concentrations					1447:1460	low minimum effective( MEC) concentrations	1419:1460	a low minimum effective( MEC) concentrations index (15 mg/mL)	1417:1477	Lower minimum effective concentrations of postbiotic were altered in food model, and substantially, a low minimum effective( MEC) concentrations index (15 mg/mL) was identified for postbiotic of L.plantarum.					
36734084	2	9	theme	soluble	169:175	arg1	Postbiotics					153:163	Postbiotics	153:163	Postbiotics	153:163	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	2	9	theme	soluble	169:175	arg1	metabolites					177:187	soluble metabolites	169:187	soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria	169:277	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	5	10	theme	postbiotics	688:698	arg1	activity					714:721	the postbiotics antibacterial activity	684:721	the postbiotics antibacterial activity	684:721	Besides, the effect of heat and pH on the postbiotics antibacterial activity was measured via the agar-well diffusion method.					
36734084	8	11	theme	lactic	1076:1081	arg1	acid					1083:1086	lactic acid	1076:1086	lactic acid	1076:1086	The antibacterial activity was mainly associated with lactic acid, laurostearic acid, and isopropylidene-3,3-dimethyl.					
36734084	11	12	theme	Lower	1317:1321	arg1	concentrations					1341:1354	Lower minimum effective concentrations	1317:1354	Lower minimum effective concentrations of postbiotic	1317:1368	Lower minimum effective concentrations of postbiotic were altered in food model, and substantially, a low minimum effective( MEC) concentrations index (15 mg/mL) was identified for postbiotic of L.plantarum.					
36734084	9	13	theme	strong	1166:1171	arg1	activity					1185:1192	strong antioxidant activity	1166:1192	strong antioxidant activity	1166:1192	Also, postbiotics showed strong antioxidant activity.					
36734084	4	14	theme	diffusion	628:636	arg1	method					638:643	agar-disk diffusion method	618:643	agar-disk diffusion method	618:643	Potential activity of postbiotics was performed via agar-disk diffusion method.					
36734084	6	15	theme	potential	840:848	arg1	scavenging					829:838	the free radical scavenging potential	812:848	the free radical scavenging potential of the postbiotics	812:867	To determine the antioxidant effect and the free radical scavenging potential of the postbiotics, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method was utilized.					
36734084	9	16	theme	antioxidant	1173:1183	arg1	activity					1185:1192	strong antioxidant activity	1166:1192	strong antioxidant activity	1166:1192	Also, postbiotics showed strong antioxidant activity.					
36734084	11	17	theme	MEC	1442:1444	arg1	concentrations					1447:1460	low minimum effective( MEC) concentrations	1419:1460	a low minimum effective( MEC) concentrations index (15 mg/mL)	1417:1477	Lower minimum effective concentrations of postbiotic were altered in food model, and substantially, a low minimum effective( MEC) concentrations index (15 mg/mL) was identified for postbiotic of L.plantarum.					
36734084	5	18	theme	heat	669:672	arg1	effect					659:664	the effect	655:664	the effect of heat and pH on the postbiotics antibacterial activity	655:721	Besides, the effect of heat and pH on the postbiotics antibacterial activity was measured via the agar-well diffusion method.					
36734084	6	19	theme	radical	821:827	arg1	scavenging					829:838	the free radical scavenging potential	812:848	the free radical scavenging potential of the postbiotics	812:867	To determine the antioxidant effect and the free radical scavenging potential of the postbiotics, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method was utilized.					
36734084	2	20	theme	biological	321:330	arg1	activity					332:339	the host increased biological activity	302:339	the host increased biological activity	302:339	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	10	21	attach	derived	1207:1213	arg2	Postbiotics					1195:1205	Postbiotics	1195:1205	Postbiotics derived from L.plantarum	1195:1230	Postbiotics derived from L.plantarum showed the highest antioxidant properties compared to L.paracasei and L.acidophilus.					
36734084	10	21	attach	derived	1207:1213	arg1	L.plantarum					1220:1230	L.plantarum	1220:1230	L.plantarum	1220:1230	Postbiotics derived from L.plantarum showed the highest antioxidant properties compared to L.paracasei and L.acidophilus.					
36734084	11	22	theme	postbiotic	1359:1368	arg1	concentrations					1341:1354	Lower minimum effective concentrations	1317:1354	Lower minimum effective concentrations of postbiotic	1317:1368	Lower minimum effective concentrations of postbiotic were altered in food model, and substantially, a low minimum effective( MEC) concentrations index (15 mg/mL) was identified for postbiotic of L.plantarum.					
36734084	4	23	theme	postbiotics	588:598	arg1	activity					576:583	Potential activity	566:583	Potential activity of postbiotics	566:598	Potential activity of postbiotics was performed via agar-disk diffusion method.					
36734084	11	24	theme	L.plantarum	1512:1522	arg1	postbiotic					1498:1507	postbiotic	1498:1507	postbiotic of L.plantarum	1498:1522	Lower minimum effective concentrations of postbiotic were altered in food model, and substantially, a low minimum effective( MEC) concentrations index (15 mg/mL) was identified for postbiotic of L.plantarum.					
36734084	6	25	theme	free	816:819	arg1	scavenging					829:838	the free radical scavenging potential	812:848	the free radical scavenging potential of the postbiotics	812:867	To determine the antioxidant effect and the free radical scavenging potential of the postbiotics, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method was utilized.					
36734084	2	26	theme	increased	311:319	arg1	activity					332:339	the host increased biological activity	302:339	the host increased biological activity	302:339	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	10	27	theme	antioxidant	1251:1261	arg1	properties					1263:1272	the highest antioxidant properties	1239:1272	the highest antioxidant properties	1239:1272	Postbiotics derived from L.plantarum showed the highest antioxidant properties compared to L.paracasei and L.acidophilus.					
36734084	3	28	theme	current	383:389	arg1	study					391:395	the current study	379:395	the current study	379:395	In the current study, the anti-Staphylococcus properties of postbiotics isolated from Lactobacillus acidophilus,L.paracasei,and L.plantarum were investigated in vitro, and pasteurized milk.					
36734084	7	29	theme	gas	984:986	arg1	spectrometry					1008:1019	gas chromatography-mass spectrometry	984:1019	gas chromatography-mass spectrometry	984:1019	The postbiotics chemical composition was identified via gas chromatography-mass spectrometry.					
36734084	3	30	attach	isolated	448:455	arg2	postbiotics					436:446	postbiotics	436:446	postbiotics isolated from Lactobacillus acidophilus,L.paracasei,and L.plantarum	436:514	In the current study, the anti-Staphylococcus properties of postbiotics isolated from Lactobacillus acidophilus,L.paracasei,and L.plantarum were investigated in vitro, and pasteurized milk.					
36734084	3	30	attach	isolated	448:455	arg1	L.paracasei					488:498	L.paracasei	488:498	L.paracasei	488:498	In the current study, the anti-Staphylococcus properties of postbiotics isolated from Lactobacillus acidophilus,L.paracasei,and L.plantarum were investigated in vitro, and pasteurized milk.					
36734084	3	30	attach	isolated	448:455	arg1	L.plantarum					504:514	L.plantarum	504:514	L.plantarum	504:514	In the current study, the anti-Staphylococcus properties of postbiotics isolated from Lactobacillus acidophilus,L.paracasei,and L.plantarum were investigated in vitro, and pasteurized milk.					
36734084	3	30	attach	isolated	448:455	arg1	acidophilus					476:486	Lactobacillus acidophilus	462:486	Lactobacillus acidophilus	462:486	In the current study, the anti-Staphylococcus properties of postbiotics isolated from Lactobacillus acidophilus,L.paracasei,and L.plantarum were investigated in vitro, and pasteurized milk.					
36734084	1	31	theme	in	114:115	arg1	circumstances					123:135	in vitro circumstances	114:135	in vitro circumstances	114:135	against Staphylococcus aureus under in vitro circumstances and food model.					
36734084	0	32	theme	growth-inhibitory	14:30	arg1	activity					32:39	the growth-inhibitory activity	10:39	the growth-inhibitory activity of postbiotics of Lactobacillus spp	10:75	Assessing the growth-inhibitory activity of postbiotics of Lactobacillus spp.					
36734084	5	33	from	effect	659:664	arg1	activity					714:721	the postbiotics antibacterial activity	684:721	the postbiotics antibacterial activity	684:721	Besides, the effect of heat and pH on the postbiotics antibacterial activity was measured via the agar-well diffusion method.					
36734084	11	34	theme	effective	1331:1339	arg1	concentrations					1341:1354	Lower minimum effective concentrations	1317:1354	Lower minimum effective concentrations of postbiotic	1317:1368	Lower minimum effective concentrations of postbiotic were altered in food model, and substantially, a low minimum effective( MEC) concentrations index (15 mg/mL) was identified for postbiotic of L.plantarum.					
36734084	2	35	attach	liberated	198:206	arg2	metabolites					177:187	soluble metabolites	169:187	soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria	169:277	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	2	35	attach	liberated	198:206	arg2	Postbiotics					153:163	Postbiotics	153:163	Postbiotics	153:163	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	2	35	attach	liberated	198:206	arg1	structure					217:225	the structure	213:225	the structure of lysing bacteria	213:244	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	0	36	theme	postbiotics	44:54	arg1	activity					32:39	the growth-inhibitory activity	10:39	the growth-inhibitory activity of postbiotics of Lactobacillus spp	10:75	Assessing the growth-inhibitory activity of postbiotics of Lactobacillus spp.					
36734084	5	37	theme	agar-well	744:752	arg1	method					764:769	the agar-well diffusion method	740:769	the agar-well diffusion method	740:769	Besides, the effect of heat and pH on the postbiotics antibacterial activity was measured via the agar-well diffusion method.					
36734084	10	38	theme	highest	1243:1249	arg1	properties					1263:1272	the highest antioxidant properties	1239:1272	the highest antioxidant properties	1239:1272	Postbiotics derived from L.plantarum showed the highest antioxidant properties compared to L.paracasei and L.acidophilus.					
36734084	13	39	theme	functional	1603:1612	arg1	characteristics					1614:1628	useful functional characteristics	1596:1628	useful functional characteristics (possible antibacterial and antioxidant)	1596:1669	postbiotic, in particular L.plantarum, may have useful functional characteristics (possible antibacterial and antioxidant) in in vitro and food model.					
36734084	3	40	theme	postbiotics	436:446	arg1	properties					422:431	the anti-Staphylococcus properties	398:431	the anti-Staphylococcus properties of postbiotics isolated from Lactobacillus acidophilus,L.paracasei,and L.plantarum	398:514	In the current study, the anti-Staphylococcus properties of postbiotics isolated from Lactobacillus acidophilus,L.paracasei,and L.plantarum were investigated in vitro, and pasteurized milk.					
36734084	5	41	theme	diffusion	754:762	arg1	method					764:769	the agar-well diffusion method	740:769	the agar-well diffusion method	740:769	Besides, the effect of heat and pH on the postbiotics antibacterial activity was measured via the agar-well diffusion method.					
36734084	13	42	dep	characteristics	1614:1628	arg1	antioxidant					1658:1668	antioxidant	1658:1668	antioxidant	1658:1668	postbiotic, in particular L.plantarum, may have useful functional characteristics (possible antibacterial and antioxidant) in in vitro and food model.					
36734084	13	42	dep	characteristics	1614:1628	arg1	antibacterial					1640:1652	antibacterial	1640:1652	antibacterial	1640:1652	postbiotic, in particular L.plantarum, may have useful functional characteristics (possible antibacterial and antioxidant) in in vitro and food model.					
36734084	8	43	theme	laurostearic	1089:1100	arg1	acid					1102:1105	laurostearic acid	1089:1105	laurostearic acid	1089:1105	The antibacterial activity was mainly associated with lactic acid, laurostearic acid, and isopropylidene-3,3-dimethyl.					
36734084	3	44	theme	anti-Staphylococcus	402:420	arg1	properties					422:431	the anti-Staphylococcus properties	398:431	the anti-Staphylococcus properties of postbiotics isolated from Lactobacillus acidophilus,L.paracasei,and L.plantarum	398:514	In the current study, the anti-Staphylococcus properties of postbiotics isolated from Lactobacillus acidophilus,L.paracasei,and L.plantarum were investigated in vitro, and pasteurized milk.					
36734084	5	45	theme	antibacterial	700:712	arg1	activity					714:721	the postbiotics antibacterial activity	684:721	the postbiotics antibacterial activity	684:721	Besides, the effect of heat and pH on the postbiotics antibacterial activity was measured via the agar-well diffusion method.					
36734084	0	46	theme	spp	73:75	arg1	postbiotics					44:54	postbiotics	44:54	postbiotics of Lactobacillus spp	44:75	Assessing the growth-inhibitory activity of postbiotics of Lactobacillus spp.					
36734084	2	47	theme	bacteria	237:244	arg1	structure					217:225	the structure	213:225	the structure of lysing bacteria	213:244	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	2	48	theme	physiological	353:365	arg1	effects					367:373	certain physiological effects	345:373	certain physiological effects	345:373	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	6	49	used	utilized	918:925	arg2	method					907:912	1,1-Diphenyl-2-picrylhydrazyl (DPPH) method	870:912	1,1-Diphenyl-2-picrylhydrazyl (DPPH) method	870:912	To determine the antioxidant effect and the free radical scavenging potential of the postbiotics, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method was utilized.					
36734084	6	50	theme	DPPH	901:904	arg1	method					907:912	1,1-Diphenyl-2-picrylhydrazyl (DPPH) method	870:912	1,1-Diphenyl-2-picrylhydrazyl (DPPH) method	870:912	To determine the antioxidant effect and the free radical scavenging potential of the postbiotics, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method was utilized.					
36734084	2	51	theme	lysing	230:235	arg1	bacteria					237:244	lysing bacteria	230:244	lysing bacteria	230:244	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	6	52	theme	antioxidant	789:799	arg1	effect					801:806	the antioxidant effect	785:806	the antioxidant effect	785:806	To determine the antioxidant effect and the free radical scavenging potential of the postbiotics, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method was utilized.					
36734084	2	53	theme	certain	345:351	arg1	effects					367:373	certain physiological effects	345:373	certain physiological effects	345:373	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	13	54	dep	in	1674:1675	arg1	vitro					1677:1681	vitro	1677:1681	vitro	1677:1681	postbiotic, in particular L.plantarum, may have useful functional characteristics (possible antibacterial and antioxidant) in in vitro and food model.					
36734084	4	55	theme	Potential	566:574	arg1	activity					576:583	Potential activity	566:583	Potential activity of postbiotics	566:598	Potential activity of postbiotics was performed via agar-disk diffusion method.					
36734084	11	56	theme	food	1386:1389	arg1	model					1391:1395	food model	1386:1395	food model	1386:1395	Lower minimum effective concentrations of postbiotic were altered in food model, and substantially, a low minimum effective( MEC) concentrations index (15 mg/mL) was identified for postbiotic of L.plantarum.					
36734084	1	57	theme	food	141:144	arg1	model					146:150	food model	141:150	food model	141:150	against Staphylococcus aureus under in vitro circumstances and food model.					
36734084	11	58	theme	minimum	1323:1329	arg1	concentrations					1341:1354	Lower minimum effective concentrations	1317:1354	Lower minimum effective concentrations of postbiotic	1317:1368	Lower minimum effective concentrations of postbiotic were altered in food model, and substantially, a low minimum effective( MEC) concentrations index (15 mg/mL) was identified for postbiotic of L.plantarum.					
36734084	13	59	contain	have	1591:1594	arg2	characteristics					1614:1628	useful functional characteristics	1596:1628	useful functional characteristics (possible antibacterial and antioxidant)	1596:1669	postbiotic, in particular L.plantarum, may have useful functional characteristics (possible antibacterial and antioxidant) in in vitro and food model.					
36734084	13	59	contain	have	1591:1594	arg1	postbiotic					1548:1557	postbiotic	1548:1557	postbiotic	1548:1557	postbiotic, in particular L.plantarum, may have useful functional characteristics (possible antibacterial and antioxidant) in in vitro and food model.					
36734084	6	60	theme	1,1-Diphenyl-2-picrylhydrazyl	870:898	arg1	method					907:912	1,1-Diphenyl-2-picrylhydrazyl (DPPH) method	870:912	1,1-Diphenyl-2-picrylhydrazyl (DPPH) method	870:912	To determine the antioxidant effect and the free radical scavenging potential of the postbiotics, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method was utilized.					
36734084	2	61	theme	live	265:268	arg1	bacteria					270:277	live bacteria	265:277	live bacteria	265:277	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	8	62	theme	antibacterial	1026:1038	arg1	activity					1040:1047	The antibacterial activity	1022:1047	The antibacterial activity	1022:1047	The antibacterial activity was mainly associated with lactic acid, laurostearic acid, and isopropylidene-3,3-dimethyl.					
36734084	2	63	dep	increased	311:319	arg1	host					306:309	host	306:309	host	306:309	Postbiotics are soluble metabolites that are liberated from the structure of lysing bacteria or are produced by live bacteria; these byproducts give the host increased biological activity and certain physiological effects.					
36734084	13	64	theme	useful	1596:1601	arg1	characteristics					1614:1628	useful functional characteristics	1596:1628	useful functional characteristics (possible antibacterial and antioxidant)	1596:1669	postbiotic, in particular L.plantarum, may have useful functional characteristics (possible antibacterial and antioxidant) in in vitro and food model.					
36734084	11	65	theme	effective	1431:1439	arg1	concentrations					1447:1460	low minimum effective( MEC) concentrations	1419:1460	a low minimum effective( MEC) concentrations index (15 mg/mL)	1417:1477	Lower minimum effective concentrations of postbiotic were altered in food model, and substantially, a low minimum effective( MEC) concentrations index (15 mg/mL) was identified for postbiotic of L.plantarum.					
36734084	1	66	dep	in	114:115	arg1	vitro					117:121	vitro	117:121	vitro	117:121	against Staphylococcus aureus under in vitro circumstances and food model.					
36734084	0	67	theme	Lactobacillus	59:71	arg1	spp					73:75	Lactobacillus spp	59:75	Lactobacillus spp	59:75	Assessing the growth-inhibitory activity of postbiotics of Lactobacillus spp.					
36734084	11	68	theme	concentrations	1447:1460	arg1	index					1462:1466	a low minimum effective( MEC) concentrations index	1417:1466	a low minimum effective( MEC) concentrations index (15 mg/mL)	1417:1477	Lower minimum effective concentrations of postbiotic were altered in food model, and substantially, a low minimum effective( MEC) concentrations index (15 mg/mL) was identified for postbiotic of L.plantarum.					
36734084	11	68	theme	concentrations	1447:1460	arg1	15 mg/mL					1469:1476	15 mg/mL	1469:1476	15 mg/mL	1469:1476	Lower minimum effective concentrations of postbiotic were altered in food model, and substantially, a low minimum effective( MEC) concentrations index (15 mg/mL) was identified for postbiotic of L.plantarum.					
36734084	7	69	theme	postbiotics	932:942	arg1	composition					953:963	The postbiotics chemical composition	928:963	The postbiotics chemical composition	928:963	The postbiotics chemical composition was identified via gas chromatography-mass spectrometry.					
37031900	2	0	theme	Assessment	539:548	arg1	score					565:569	Assessment System (ICDAS) score 4 and 5	539:577	score	565:569	MATERIALS AND METHODS The study included human mandibular molars assessed on International Caries Detection and Assessment System (ICDAS) score 4 and 5.					
37031900	18	1	theme	antimicrobial	2405:2417	arg1	efficacy					2419:2426	better antimicrobial efficacy	2398:2426	better antimicrobial efficacy	2398:2426	However, it is worth noting that Fotoenticine exhibited better antimicrobial efficacy against S. mutans.					
37031900	12	2	theme	strength	1778:1785	arg1	values					1787:1792	highest bond strength values	1765:1792	highest bond strength values (21.48±1.39 MPa)	1765:1809	It was also discovered that CHX unveiled highest bond strength values (21.48±1.39 MPa).					
37031900	12	2	theme	strength	1778:1785	arg1	MPa					1806:1808	21.48±1.39 MPa	1795:1808	21.48±1.39 MPa	1795:1808	It was also discovered that CHX unveiled highest bond strength values (21.48±1.39 MPa).					
37031900	16	3	theme	SBS	2154:2156	arg1	outcomes					2142:2149	comparable outcomes	2131:2149	comparable outcomes of SBS	2131:2156	However, group 3 (Fotoenticine) (16.28±0.51 MPa) and group 2 demonstrated comparable outcomes of SBS.					
37031900	10	4	dep	test	1520:1523	arg1	RESULTS					1525:1531	RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10)	1525:1617	the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10)	1487:1617	Data on the survival rate of S. mutans were compared between groups using the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10).					
37031900	1	5	theme	resin	238:242	arg1	restoration					253:263	resin adhesive restoration	238:263	resin adhesive restoration bonded to carious affected dentin (CAD)	238:303	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	9	6	used	used	1393:1396	arg2	tests					1382:1386	ANOVA and Tukey multiple comparison tests	1346:1386	ANOVA and Tukey multiple comparison tests	1346:1386	ANOVA and Tukey multiple comparison tests were used to assess SBS.					
37031900	14	7	theme	comparison	1892:1901	arg1	analysis					1903:1910	Intergroup comparison analysis	1881:1910	Intergroup comparison analysis	1881:1910	Intergroup comparison analysis presented that group 1, and group 4 (Co2 laser) (17.76±0.41 MPa) displayed no significant difference in their bond integrity achieved.					
37031900	4	8	theme	root	776:779	arg1	embedded					795:802	embedded	795:802	embedded	795:802	The root sections were embedded in polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface.					
37031900	4	8	theme	root	776:779	arg1	sections					781:788	The root sections	772:788	The root sections	772:788	The root sections were embedded in polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface.					
37031900	13	9	theme	lowest	1852:1857	arg1	SBS					1859:1861	lowest SBS	1852:1861	lowest SBS (11.01±1.00 MPa)	1852:1878	Nevertheless, Group 2 (Chitosan) showed lowest SBS (11.01±1.00 MPa).					
37031900	13	9	theme	lowest	1852:1857	arg1	MPa					1875:1877	11.01±1.00 MPa	1864:1877	11.01±1.00 MPa	1864:1877	Nevertheless, Group 2 (Chitosan) showed lowest SBS (11.01±1.00 MPa).					
37031900	1	10	theme	survival	179:186	arg1	rate					188:191	the survival rate	175:191	the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD)	175:303	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	12	11	theme	highest	1765:1771	arg1	values					1787:1792	highest bond strength values	1765:1792	highest bond strength values (21.48±1.39 MPa)	1765:1809	It was also discovered that CHX unveiled highest bond strength values (21.48±1.39 MPa).					
37031900	12	11	theme	highest	1765:1771	arg1	MPa					1806:1808	21.48±1.39 MPa	1795:1808	21.48±1.39 MPa	1795:1808	It was also discovered that CHX unveiled highest bond strength values (21.48±1.39 MPa).					
37031900	2	12	theme	International	504:516	arg1	Detection					525:533	International Caries Detection	504:533	International Caries Detection	504:533	MATERIALS AND METHODS The study included human mandibular molars assessed on International Caries Detection and Assessment System (ICDAS) score 4 and 5.					
37031900	3	13	theme	central	660:666	arg1	fossa					668:672	the central fossa	656:672	the central fossa	656:672	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
37031900	0	14	theme	resin	116:120	arg1	restoration					122:132	resin restoration	116:132	resin restoration	116:132	Different cavity disinfectant efficacy against S.Mutans and shear bond strength of caries affected dentin bonded to resin restoration.					
37031900	4	15	theme	S.mutans	867:874	arg1	biofilm					876:882	S.mutans biofilm	867:882	S.mutans biofilm	867:882	The root sections were embedded in polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface.					
37031900	4	16	theme	methacrylate	818:829	arg1	resin					839:843	polymethyl methacrylate acrylic resin	807:843	polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface	807:901	The root sections were embedded in polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface.					
37031900	3	17	dep	cut	620:622	arg1	off					624:626	off	624:626	off	624:626	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
37031900	17	18	theme	positive	2260:2267	arg1	impact					2269:2274	a positive impact	2258:2274	a positive impact on the SBS of resin composite	2258:2304	(p>0.05) CONCLUSION: The use of CHX and CO2 lasers as disinfectants on the CAD surface resulted in a positive impact on the SBS of resin composite, according to the study's findings.					
37031900	10	19	theme	survival	1425:1432	arg1	rate					1434:1437	the survival rate	1421:1437	the survival rate of S. mutans	1421:1450	Data on the survival rate of S. mutans were compared between groups using the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10).					
37031900	4	20	from	resin	839:843	arg1	embedded					795:802	embedded	795:802	embedded	795:802	The root sections were embedded in polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface.					
37031900	4	20	from	resin	839:843	arg1	sections					781:788	The root sections	772:788	The root sections	772:788	The root sections were embedded in polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface.					
37031900	8	21	theme	bond	1309:1312	arg1	integrity					1314:1322	bond integrity	1309:1322	bond integrity	1309:1322	Thermoocycling of the samples was performed and a universal testing machine (UTM) and Stereomicroscope were used to identify bond integrity and type of fracture.					
37031900	0	22	theme	bond	66:69	arg1	strength					71:78	shear bond strength	60:78	shear bond strength	60:78	Different cavity disinfectant efficacy against S.Mutans and shear bond strength of caries affected dentin bonded to resin restoration.					
37031900	9	23	theme	ANOVA	1346:1350	arg1	tests					1382:1386	ANOVA and Tukey multiple comparison tests	1346:1386	ANOVA and Tukey multiple comparison tests	1346:1386	ANOVA and Tukey multiple comparison tests were used to assess SBS.					
37031900	14	24	theme	Intergroup	1881:1890	arg1	analysis					1903:1910	Intergroup comparison analysis	1881:1910	Intergroup comparison analysis	1881:1910	Intergroup comparison analysis presented that group 1, and group 4 (Co2 laser) (17.76±0.41 MPa) displayed no significant difference in their bond integrity achieved.					
37031900	2	25	theme	Caries	518:523	arg1	Detection					525:533	International Caries Detection	504:533	International Caries Detection	504:533	MATERIALS AND METHODS The study included human mandibular molars assessed on International Caries Detection and Assessment System (ICDAS) score 4 and 5.					
37031900	9	26	theme	Tukey	1356:1360	arg1	tests					1382:1386	ANOVA and Tukey multiple comparison tests	1346:1386	ANOVA and Tukey multiple comparison tests	1346:1386	ANOVA and Tukey multiple comparison tests were used to assess SBS.					
37031900	2	27	theme	mandibular	474:483	arg1	molars					485:490	human mandibular molars	468:490	human mandibular molars	468:490	MATERIALS AND METHODS The study included human mandibular molars assessed on International Caries Detection and Assessment System (ICDAS) score 4 and 5.					
37031900	14	28	theme	bond	2022:2025	arg1	integrity					2027:2035	their bond integrity	2016:2035	their bond integrity achieved	2016:2044	Intergroup comparison analysis presented that group 1, and group 4 (Co2 laser) (17.76±0.41 MPa) displayed no significant difference in their bond integrity achieved.					
37031900	3	29	theme	cementoenamel	742:754	arg1	junction					756:763	the tooth cementoenamel junction	732:763	the tooth cementoenamel junction (CEJ)	732:769	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
37031900	3	29	theme	cementoenamel	742:754	arg1	CEJ					766:768	CEJ	766:768	CEJ	766:768	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
37031900	1	30	theme	different	317:325	arg1	Fotoenticine®					359:371	Fotoenticine®	359:371	Fotoenticine®	359:371	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	30	theme	different	317:325	arg1	laser					382:386	CO2 laser	378:386	CO2 laser	378:386	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	30	theme	different	317:325	arg1	Chitosan					349:356	Chitosan	349:356	Chitosan	349:356	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	30	theme	different	317:325	arg1	disinfectants					334:346	different cavity disinfectants	317:346	different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser)	317:387	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	9	31	theme	comparison	1371:1380	arg1	tests					1382:1386	ANOVA and Tukey multiple comparison tests	1346:1386	ANOVA and Tukey multiple comparison tests	1346:1386	ANOVA and Tukey multiple comparison tests were used to assess SBS.					
37031900	4	32	theme	CAD	891:893	arg1	surface					895:901	the CAD surface	887:901	the CAD surface	887:901	The root sections were embedded in polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface.					
37031900	11	33	theme	treated	1693:1699	arg1	0.25±0.06					1712:1720	0.25±0.06	1712:1720	0.25±0.06	1712:1720	However, lowest survival rate was demonstrated by Group 3 (Fotoenticine) treated specimens (0.25±0.06).					
37031900	11	33	theme	treated	1693:1699	arg1	specimens					1701:1709	treated specimens	1693:1709	Group 3 (Fotoenticine) treated specimens (0.25±0.06)	1670:1721	However, lowest survival rate was demonstrated by Group 3 (Fotoenticine) treated specimens (0.25±0.06).					
37031900	7	34	theme	restorative	1162:1172	arg1	material					1174:1181	a composite restorative material	1150:1181	a composite restorative material	1150:1181	S.mutans survival rate was assessed and CAD was restored with a composite restorative material.					
37031900	8	35	theme	samples	1206:1212	arg1	Thermoocycling					1184:1197	Thermoocycling	1184:1197	Thermoocycling of the samples	1184:1212	Thermoocycling of the samples was performed and a universal testing machine (UTM) and Stereomicroscope were used to identify bond integrity and type of fracture.					
37031900	14	36	theme	significant	1990:2000	arg1	difference					2002:2011	no significant difference	1987:2011	no significant difference	1987:2011	Intergroup comparison analysis presented that group 1, and group 4 (Co2 laser) (17.76±0.41 MPa) displayed no significant difference in their bond integrity achieved.					
37031900	0	37	theme	Different	0:8	arg1	efficacy					30:37	Different cavity disinfectant efficacy	0:37	Different cavity disinfectant efficacy against S.Mutans and shear bond strength of caries	0:88	Different cavity disinfectant efficacy against S.Mutans and shear bond strength of caries affected dentin bonded to resin restoration.					
37031900	1	38	theme	adhesive	244:251	arg1	restoration					253:263	resin adhesive restoration	238:263	resin adhesive restoration bonded to carious affected dentin (CAD)	238:303	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	11	39	dep	Group	1670:1674	arg1	0.25±0.06					1712:1720	0.25±0.06	1712:1720	0.25±0.06	1712:1720	However, lowest survival rate was demonstrated by Group 3 (Fotoenticine) treated specimens (0.25±0.06).					
37031900	11	39	dep	Group	1670:1674	arg1	specimens					1701:1709	treated specimens	1693:1709	Group 3 (Fotoenticine) treated specimens (0.25±0.06)	1670:1721	However, lowest survival rate was demonstrated by Group 3 (Fotoenticine) treated specimens (0.25±0.06).					
37031900	10	40	theme	nonparametric	1491:1503	arg1	test					1520:1523	the nonparametric Kruskal-Wallis test	1487:1523	the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10)	1487:1617	Data on the survival rate of S. mutans were compared between groups using the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10).					
37031900	0	41	theme	disinfectant	17:28	arg1	efficacy					30:37	Different cavity disinfectant efficacy	0:37	Different cavity disinfectant efficacy against S.Mutans and shear bond strength of caries	0:88	Different cavity disinfectant efficacy against S.Mutans and shear bond strength of caries affected dentin bonded to resin restoration.					
37031900	1	42	theme	bonded	265:270	arg1	restoration					253:263	resin adhesive restoration	238:263	resin adhesive restoration bonded to carious affected dentin (CAD)	238:303	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	43	theme	carious	275:281	arg1	CAD					300:302	CAD	300:302	CAD	300:302	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	43	theme	carious	275:281	arg1	dentin					292:297	carious affected dentin	275:297	carious affected dentin (CAD)	275:303	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	8	44	theme	testing	1244:1250	arg1	UTM					1261:1263	UTM	1261:1263	UTM	1261:1263	Thermoocycling of the samples was performed and a universal testing machine (UTM) and Stereomicroscope were used to identify bond integrity and type of fracture.					
37031900	8	44	theme	testing	1244:1250	arg1	machine					1252:1258	a universal testing machine	1232:1258	a universal testing machine (UTM)	1232:1264	Thermoocycling of the samples was performed and a universal testing machine (UTM) and Stereomicroscope were used to identify bond integrity and type of fracture.					
37031900	2	45	dep	MATERIALS	427:435	arg1	included					459:466	included	459:466	included human mandibular molars	459:490	MATERIALS AND METHODS The study included human mandibular molars assessed on International Caries Detection and Assessment System (ICDAS) score 4 and 5.					
37031900	3	46	theme	crown	610:614	arg1	part					589:592	The cusp part	580:592	The cusp part of the clinical crown	580:614	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
37031900	11	47	theme	survival	1636:1643	arg1	rate					1645:1648	lowest survival rate	1629:1648	lowest survival rate	1629:1648	However, lowest survival rate was demonstrated by Group 3 (Fotoenticine) treated specimens (0.25±0.06).					
37031900	1	48	theme	shear	209:213	arg1	strength					220:227	shear bond strength	209:227	shear bond strength	209:227	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	5	49	theme	disinfection	987:998	arg1	type					979:982	the type	975:982	the type of disinfection	975:998	Specimens were arbitrarily allocated into four groups(n = 10) based on the type of disinfection.					
37031900	1	50	theme	existing	144:151	arg1	study					153:157	The existing study	140:157	The existing study	140:157	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	17	51	dep	CONCLUSION	2168:2177	arg1	lasers					2203:2208	lasers	2203:2208	lasers as disinfectants on the CAD surface resulted in a positive impact on the SBS of resin composite, according to the study's findings	2203:2339	(p>0.05) CONCLUSION: The use of CHX and CO2 lasers as disinfectants on the CAD surface resulted in a positive impact on the SBS of resin composite, according to the study's findings.					
37031900	1	52	theme	strength	220:227	arg1	rate					188:191	the survival rate	175:191	the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD)	175:303	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	10	53	from	Data	1413:1416	arg1	rate					1434:1437	the survival rate	1421:1437	the survival rate of S. mutans	1421:1450	Data on the survival rate of S. mutans were compared between groups using the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10).					
37031900	7	54	theme	S.mutans	1088:1095	arg1	rate					1106:1109	S.mutans survival rate	1088:1109	S.mutans survival rate	1088:1109	S.mutans survival rate was assessed and CAD was restored with a composite restorative material.					
37031900	17	55	theme	CHX	2191:2193	arg1	use					2184:2186	The use	2180:2186	The use of CHX and CO2	2180:2201	(p>0.05) CONCLUSION: The use of CHX and CO2 lasers as disinfectants on the CAD surface resulted in a positive impact on the SBS of resin composite, according to the study's findings.					
37031900	17	56	theme	composite	2296:2304	arg1	SBS					2283:2285	the SBS	2279:2285	the SBS of resin composite	2279:2304	(p>0.05) CONCLUSION: The use of CHX and CO2 lasers as disinfectants on the CAD surface resulted in a positive impact on the SBS of resin composite, according to the study's findings.					
37031900	2	57	theme	ICDAS	558:562	arg1	score					565:569	Assessment System (ICDAS) score 4 and 5	539:577	score	565:569	MATERIALS AND METHODS The study included human mandibular molars assessed on International Caries Detection and Assessment System (ICDAS) score 4 and 5.					
37031900	17	58	from	disinfectants	2213:2225	arg1	surface					2238:2244	the CAD surface	2230:2244	the CAD surface	2230:2244	(p>0.05) CONCLUSION: The use of CHX and CO2 lasers as disinfectants on the CAD surface resulted in a positive impact on the SBS of resin composite, according to the study's findings.					
37031900	17	59	theme	CO2	2199:2201	arg1	use					2184:2186	The use	2180:2186	The use of CHX and CO2	2180:2201	(p>0.05) CONCLUSION: The use of CHX and CO2 lasers as disinfectants on the CAD surface resulted in a positive impact on the SBS of resin composite, according to the study's findings.					
37031900	14	60	theme	Co2	1949:1951	arg1	group					1940:1944	group 4	1940:1946	group 4 (Co2 laser) (17.76±0.41 MPa)	1940:1975	Intergroup comparison analysis presented that group 1, and group 4 (Co2 laser) (17.76±0.41 MPa) displayed no significant difference in their bond integrity achieved.					
37031900	14	60	theme	Co2	1949:1951	arg1	laser					1953:1957	Co2 laser	1949:1957	Co2 laser	1949:1957	Intergroup comparison analysis presented that group 1, and group 4 (Co2 laser) (17.76±0.41 MPa) displayed no significant difference in their bond integrity achieved.					
37031900	18	61	theme	better	2398:2403	arg1	efficacy					2419:2426	better antimicrobial efficacy	2398:2426	better antimicrobial efficacy	2398:2426	However, it is worth noting that Fotoenticine exhibited better antimicrobial efficacy against S. mutans.					
37031900	17	62	theme	resin	2290:2294	arg1	composite					2296:2304	resin composite	2290:2304	resin composite	2290:2304	(p>0.05) CONCLUSION: The use of CHX and CO2 lasers as disinfectants on the CAD surface resulted in a positive impact on the SBS of resin composite, according to the study's findings.					
37031900	12	63	theme	bond	1773:1776	arg1	values					1787:1792	highest bond strength values	1765:1792	highest bond strength values (21.48±1.39 MPa)	1765:1809	It was also discovered that CHX unveiled highest bond strength values (21.48±1.39 MPa).					
37031900	12	63	theme	bond	1773:1776	arg1	MPa					1806:1808	21.48±1.39 MPa	1795:1808	21.48±1.39 MPa	1795:1808	It was also discovered that CHX unveiled highest bond strength values (21.48±1.39 MPa).					
37031900	2	64	theme	System	550:555	arg1	score					565:569	Assessment System (ICDAS) score 4 and 5	539:577	score	565:569	MATERIALS AND METHODS The study included human mandibular molars assessed on International Caries Detection and Assessment System (ICDAS) score 4 and 5.					
37031900	10	65	theme	survival	1593:1600	arg1	0.65±0.10					1608:1616	0.65±0.10	1608:1616	0.65±0.10	1608:1616	Data on the survival rate of S. mutans were compared between groups using the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10).					
37031900	10	65	theme	survival	1593:1600	arg1	rate					1602:1605	the highest survival rate	1581:1605	the highest survival rate (0.65±0.10)	1581:1617	Data on the survival rate of S. mutans were compared between groups using the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10).					
37031900	8	66	used	used	1292:1295	arg2	UTM					1261:1263	UTM	1261:1263	UTM	1261:1263	Thermoocycling of the samples was performed and a universal testing machine (UTM) and Stereomicroscope were used to identify bond integrity and type of fracture.					
37031900	8	66	used	used	1292:1295	arg2	machine					1252:1258	a universal testing machine	1232:1258	a universal testing machine (UTM)	1232:1264	Thermoocycling of the samples was performed and a universal testing machine (UTM) and Stereomicroscope were used to identify bond integrity and type of fracture.					
37031900	8	66	used	used	1292:1295	arg2	Stereomicroscope					1270:1285	Stereomicroscope	1270:1285	Stereomicroscope	1270:1285	Thermoocycling of the samples was performed and a universal testing machine (UTM) and Stereomicroscope were used to identify bond integrity and type of fracture.					
37031900	10	67	theme	highest	1585:1591	arg1	0.65±0.10					1608:1616	0.65±0.10	1608:1616	0.65±0.10	1608:1616	Data on the survival rate of S. mutans were compared between groups using the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10).					
37031900	10	67	theme	highest	1585:1591	arg1	rate					1602:1605	the highest survival rate	1581:1605	the highest survival rate (0.65±0.10)	1581:1617	Data on the survival rate of S. mutans were compared between groups using the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10).					
37031900	3	68	dep	till	727:730	arg1	junction					756:763	the tooth cementoenamel junction	732:763	the tooth cementoenamel junction (CEJ)	732:769	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
37031900	3	68	dep	till	727:730	arg1	CEJ					766:768	CEJ	766:768	CEJ	766:768	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
37031900	1	69	theme	S.mutans	196:203	arg1	rate					188:191	the survival rate	175:191	the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD)	175:303	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	11	70	theme	lowest	1629:1634	arg1	rate					1645:1648	lowest survival rate	1629:1648	lowest survival rate	1629:1648	However, lowest survival rate was demonstrated by Group 3 (Fotoenticine) treated specimens (0.25±0.06).					
37031900	4	71	theme	polymethyl	807:816	arg1	resin					839:843	polymethyl methacrylate acrylic resin	807:843	polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface	807:901	The root sections were embedded in polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface.					
37031900	1	72	theme	CO2	378:380	arg1	disinfectants					334:346	different cavity disinfectants	317:346	different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser)	317:387	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	72	theme	CO2	378:380	arg1	laser					382:386	CO2 laser	378:386	CO2 laser	378:386	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	4	73	theme	acrylic	831:837	arg1	resin					839:843	polymethyl methacrylate acrylic resin	807:843	polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface	807:901	The root sections were embedded in polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface.					
37031900	3	74	theme	tooth	736:740	arg1	junction					756:763	the tooth cementoenamel junction	732:763	the tooth cementoenamel junction (CEJ)	732:769	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
37031900	3	74	theme	tooth	736:740	arg1	CEJ					766:768	CEJ	766:768	CEJ	766:768	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
37031900	3	75	theme	till	727:730	arg1	coolant					719:725	water coolant	713:725	water coolant till the tooth cementoenamel junction (CEJ)	713:769	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
37031900	0	76	theme	caries	83:88	arg1	S.Mutans					47:54	S.Mutans	47:54	S.Mutans	47:54	Different cavity disinfectant efficacy against S.Mutans and shear bond strength of caries affected dentin bonded to resin restoration.					
37031900	0	76	theme	caries	83:88	arg1	strength					71:78	shear bond strength	60:78	shear bond strength	60:78	Different cavity disinfectant efficacy against S.Mutans and shear bond strength of caries affected dentin bonded to resin restoration.					
37031900	4	77	from	embedded	795:802	arg1	resin					839:843	polymethyl methacrylate acrylic resin	807:843	polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface	807:901	The root sections were embedded in polymethyl methacrylate acrylic resin followed by culturing S.mutans biofilm on the CAD surface.					
37031900	8	78	theme	fracture	1336:1343	arg1	type					1328:1331	type	1328:1331	type	1328:1331	Thermoocycling of the samples was performed and a universal testing machine (UTM) and Stereomicroscope were used to identify bond integrity and type of fracture.					
37031900	8	78	theme	fracture	1336:1343	arg1	integrity					1314:1322	bond integrity	1309:1322	bond integrity	1309:1322	Thermoocycling of the samples was performed and a universal testing machine (UTM) and Stereomicroscope were used to identify bond integrity and type of fracture.					
37031900	9	79	theme	multiple	1362:1369	arg1	tests					1382:1386	ANOVA and Tukey multiple comparison tests	1346:1386	ANOVA and Tukey multiple comparison tests	1346:1386	ANOVA and Tukey multiple comparison tests were used to assess SBS.					
37031900	2	80	theme	human	468:472	arg1	molars					485:490	human mandibular molars	468:490	human mandibular molars	468:490	MATERIALS AND METHODS The study included human mandibular molars assessed on International Caries Detection and Assessment System (ICDAS) score 4 and 5.					
37031900	1	81	dep	AIMS	135:138	arg1	aimed					159:163	aimed	159:163	aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX)	159:424	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	82	theme	cavity	327:332	arg1	Fotoenticine®					359:371	Fotoenticine®	359:371	Fotoenticine®	359:371	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	82	theme	cavity	327:332	arg1	laser					382:386	CO2 laser	378:386	CO2 laser	378:386	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	82	theme	cavity	327:332	arg1	Chitosan					349:356	Chitosan	349:356	Chitosan	349:356	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	82	theme	cavity	327:332	arg1	disinfectants					334:346	different cavity disinfectants	317:346	different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser)	317:387	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	7	83	theme	composite	1152:1160	arg1	material					1174:1181	a composite restorative material	1150:1181	a composite restorative material	1150:1181	S.mutans survival rate was assessed and CAD was restored with a composite restorative material.					
37031900	17	84	theme	CAD	2234:2236	arg1	surface					2238:2244	the CAD surface	2230:2244	the CAD surface	2230:2244	(p>0.05) CONCLUSION: The use of CHX and CO2 lasers as disinfectants on the CAD surface resulted in a positive impact on the SBS of resin composite, according to the study's findings.					
37031900	0	85	theme	cavity	10:15	arg1	efficacy					30:37	Different cavity disinfectant efficacy	0:37	Different cavity disinfectant efficacy against S.Mutans and shear bond strength of caries	0:88	Different cavity disinfectant efficacy against S.Mutans and shear bond strength of caries affected dentin bonded to resin restoration.					
37031900	10	86	theme	mutans	1445:1450	arg1	rate					1434:1437	the survival rate	1421:1437	the survival rate of S. mutans	1421:1450	Data on the survival rate of S. mutans were compared between groups using the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10).					
37031900	1	87	theme	restoration	253:263	arg1	strength					220:227	shear bond strength	209:227	shear bond strength	209:227	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	87	theme	restoration	253:263	arg1	S.mutans					196:203	S.mutans	196:203	S.mutans	196:203	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	87	theme	restoration	253:263	arg1	SBS					230:232	SBS	230:232	SBS	230:232	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	17	88	from	impact	2269:2274	arg1	SBS					2283:2285	the SBS	2279:2285	the SBS of resin composite	2279:2304	(p>0.05) CONCLUSION: The use of CHX and CO2 lasers as disinfectants on the CAD surface resulted in a positive impact on the SBS of resin composite, according to the study's findings.					
37031900	3	89	theme	cusp	584:587	arg1	part					589:592	The cusp part	580:592	The cusp part of the clinical crown	580:614	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
37031900	10	90	dep	RESULTS	1525:1531	arg1	revealed					1543:1550	revealed	1543:1550	revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10)	1543:1617	Data on the survival rate of S. mutans were compared between groups using the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10).					
37031900	1	91	dep	disinfectants	334:346	arg1	Fotoenticine®					359:371	Fotoenticine®	359:371	Fotoenticine®	359:371	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	91	dep	disinfectants	334:346	arg1	disinfectants					334:346	different cavity disinfectants	317:346	different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser)	317:387	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	91	dep	disinfectants	334:346	arg1	Chitosan					349:356	Chitosan	349:356	Chitosan	349:356	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	91	dep	disinfectants	334:346	arg1	laser					382:386	CO2 laser	378:386	CO2 laser	378:386	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	0	92	theme	shear	60:64	arg1	strength					71:78	shear bond strength	60:78	shear bond strength	60:78	Different cavity disinfectant efficacy against S.Mutans and shear bond strength of caries affected dentin bonded to resin restoration.					
37031900	1	93	theme	affected	283:290	arg1	CAD					300:302	CAD	300:302	CAD	300:302	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	1	93	theme	affected	283:290	arg1	dentin					292:297	carious affected dentin	275:297	carious affected dentin (CAD)	275:303	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	3	94	theme	clinical	601:608	arg1	crown					610:614	the clinical crown	597:614	the clinical crown	597:614	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
37031900	8	95	theme	universal	1234:1242	arg1	UTM					1261:1263	UTM	1261:1263	UTM	1261:1263	Thermoocycling of the samples was performed and a universal testing machine (UTM) and Stereomicroscope were used to identify bond integrity and type of fracture.					
37031900	8	95	theme	universal	1234:1242	arg1	machine					1252:1258	a universal testing machine	1232:1258	a universal testing machine (UTM)	1232:1264	Thermoocycling of the samples was performed and a universal testing machine (UTM) and Stereomicroscope were used to identify bond integrity and type of fracture.					
37031900	7	96	theme	survival	1097:1104	arg1	rate					1106:1109	S.mutans survival rate	1088:1109	S.mutans survival rate	1088:1109	S.mutans survival rate was assessed and CAD was restored with a composite restorative material.					
37031900	10	97	theme	Kruskal-Wallis	1505:1518	arg1	test					1520:1523	the nonparametric Kruskal-Wallis test	1487:1523	the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10)	1487:1617	Data on the survival rate of S. mutans were compared between groups using the nonparametric Kruskal-Wallis test RESULTS: Outcomes revealed that Group 1 (CHX) displayed the highest survival rate (0.65±0.10).					
37031900	1	98	theme	bond	215:218	arg1	strength					220:227	shear bond strength	209:227	shear bond strength	209:227	AIMS The existing study aimed to assess the survival rate of S.mutans and shear bond strength (SBS) of resin adhesive restoration bonded to carious affected dentin (CAD) after using different cavity disinfectants (Chitosan, Fotoenticine®, and CO2 laser) in comparison to Chlorhexidine (CHX).					
37031900	6	99	theme	%	1011:1011	arg1	CHX					1013:1015	2% CHX	1010:1015	2% CHX	1010:1015	Group 1 (2% CHX), Group 2 (Chitosan), Group 3 (Fotoenticine), and Group 4 (CO2 laser).					
37031900	6	99	theme	%	1011:1011	arg1	Group					1001:1005	Group 1	1001:1007	Group 1 (2% CHX)	1001:1016	Group 1 (2% CHX), Group 2 (Chitosan), Group 3 (Fotoenticine), and Group 4 (CO2 laser).					
37031900	16	100	theme	comparable	2131:2140	arg1	outcomes					2142:2149	comparable outcomes	2131:2149	comparable outcomes of SBS	2131:2156	However, group 3 (Fotoenticine) (16.28±0.51 MPa) and group 2 demonstrated comparable outcomes of SBS.					
37031900	3	101	theme	water	713:717	arg1	coolant					719:725	water coolant	713:725	water coolant till the tooth cementoenamel junction (CEJ)	713:769	The cusp part of the clinical crown was cut off until the reduction reaches the central fossa while being continuously supplied with water coolant till the tooth cementoenamel junction (CEJ).					
35288581	4	0	theme	significant	943:953	arg1	increase					966:973	a significant (p < 0.05) increase	941:973	a significant (p < 0.05) increase	941:973	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	6	1	theme	catechol	1176:1183	arg1	activity					1193:1200	catechol oxidase activity	1176:1200	catechol oxidase activity of the tomato extract across the different treatment groups	1176:1260	No significant effect was observed in catechol oxidase activity of the tomato extract across the different treatment groups.					
35288581	7	2	with	consistent	1334:1343	arg1	load					1381:1384	avocado pear peel polyphenolic load	1350:1384	avocado pear peel polyphenolic load in coated tomato fruits	1350:1408	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	9	3	theme	activated	1763:1771	arg1	coating					1788:1794	polyphenolic activated keratin-starch coating	1750:1794	polyphenolic activated keratin-starch coating	1750:1794	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	9	3	theme	activated	1763:1771	arg1	method					1866:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	2	4	from	temperature	630:640	arg1	6 days					592:597	6 days	592:597	6 days of storage at room (25 ± 2 °C) temperature	592:640	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	1	5	theme	increased	293:301	arg1	attention					303:311	increased attention	293:311	increased attention due to their biodegradability, eco-friendliness and sustainability	293:378	In recent times, the application of protein-based bio-composite edible films in postharvest preservation of food and agricultural products is attracting increased attention due to their biodegradability, eco-friendliness and sustainability.					
35288581	4	6	theme	titratable	915:924	arg1	acidity					926:932	titratable acidity	915:932	titratable acidity	915:932	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	1	7	theme	protein-based	176:188	arg1	films					211:215	protein-based bio-composite edible films	176:215	protein-based bio-composite edible films	176:215	In recent times, the application of protein-based bio-composite edible films in postharvest preservation of food and agricultural products is attracting increased attention due to their biodegradability, eco-friendliness and sustainability.					
35288581	6	8	theme	treatment	1245:1253	arg1	groups					1255:1260	the different treatment groups	1231:1260	the different treatment groups	1231:1260	No significant effect was observed in catechol oxidase activity of the tomato extract across the different treatment groups.					
35288581	1	9	theme	edible	204:209	arg1	films					211:215	protein-based bio-composite edible films	176:215	protein-based bio-composite edible films	176:215	In recent times, the application of protein-based bio-composite edible films in postharvest preservation of food and agricultural products is attracting increased attention due to their biodegradability, eco-friendliness and sustainability.					
35288581	2	10	theme	room	613:616	arg1	temperature					630:640	room (25 ± 2 °C) temperature	613:640	room (25 ± 2 °C) temperature	613:640	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	7	11	from	load	1381:1384	arg1	fruits					1403:1408	coated tomato fruits	1389:1408	coated tomato fruits	1389:1408	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	3	12	with	formation	701:709	arg1	degree					721:726	high degree	716:726	high degree of compatibility and homogeneity	716:759	The SEM/EDX and FTIR results revealed the successful film formation with high degree of compatibility and homogeneity.					
35288581	2	13	theme	storage	602:608	arg1	6 days					592:597	6 days	592:597	6 days of storage at room (25 ± 2 °C) temperature	592:640	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	8	14	theme	weight	1555:1560	arg1	loss					1562:1565	spoilage-induce weight loss	1539:1565	spoilage-induce weight loss	1539:1565	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	4	15	theme	6-day	774:778	arg1	loss					793:796	Following a 6-day post-coating loss	762:796	Following a 6-day post-coating loss in weight of the coated tomato fruits	762:834	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	5	16	theme	extract-loaded	1116:1129	arg1	films					1131:1135	the avocado pear peel polyphenolic extract-loaded films	1081:1135	the avocado pear peel polyphenolic extract-loaded films	1081:1135	Ascorbic acid and lycopene contents were significantly (p < 0.05) higher in the avocado pear peel polyphenolic extract-loaded films.					
35288581	2	17	theme	25 ± 2 °C	619:627	arg1	temperature					630:640	room (25 ± 2 °C) temperature	613:640	room (25 ± 2 °C) temperature	613:640	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	5	18	theme	Ascorbic	1005:1012	arg1	acid					1014:1017	Ascorbic acid	1005:1017	Ascorbic acid	1005:1017	Ascorbic acid and lycopene contents were significantly (p < 0.05) higher in the avocado pear peel polyphenolic extract-loaded films.					
35288581	2	19	theme	infected	563:570	arg1	fruits					579:584	fungal infected tomato fruits	556:584	fungal infected tomato fruits	556:584	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	0	20	from	coating	105:111	arg1	fruits					132:137	infected tomato fruits	116:137	infected tomato fruits	116:137	Fabrication, characterization and antifungal evaluation of polyphenolic extract activated keratin starch coating on infected tomato fruits.					
35288581	7	21	theme	dependent	1315:1323	arg1	increase					1325:1332	a dose dependent increase	1308:1332	a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits	1308:1408	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	4	22	theme	Following	762:770	arg1	loss					793:796	Following a 6-day post-coating loss	762:796	Following a 6-day post-coating loss in weight of the coated tomato fruits	762:834	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	0	23	theme	keratin	90:96	arg1	coating					105:111	keratin starch coating	90:111	keratin starch coating on infected tomato fruits	90:137	Fabrication, characterization and antifungal evaluation of polyphenolic extract activated keratin starch coating on infected tomato fruits.					
35288581	9	24	theme	fruits	1909:1914	arg1	life					1894:1897	the shelf life	1884:1897	the shelf life of tomato fruits	1884:1914	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	4	25	theme	tomato	822:827	arg1	fruits					829:834	the coated tomato fruits	811:834	the coated tomato fruits	811:834	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	1	26	theme	recent	143:148	arg1	times					150:154	recent times	143:154	recent times	143:154	In recent times, the application of protein-based bio-composite edible films in postharvest preservation of food and agricultural products is attracting increased attention due to their biodegradability, eco-friendliness and sustainability.					
35288581	6	27	located	observed	1164:1171	arg2	effect					1153:1158	No significant effect	1138:1158	No significant effect	1138:1158	No significant effect was observed in catechol oxidase activity of the tomato extract across the different treatment groups.					
35288581	6	27	located	observed	1164:1171	arg1	activity					1193:1200	catechol oxidase activity	1176:1200	catechol oxidase activity of the tomato extract across the different treatment groups	1176:1260	No significant effect was observed in catechol oxidase activity of the tomato extract across the different treatment groups.					
35288581	0	28	theme	infected	116:123	arg1	fruits					132:137	infected tomato fruits	116:137	infected tomato fruits	116:137	Fabrication, characterization and antifungal evaluation of polyphenolic extract activated keratin starch coating on infected tomato fruits.					
35288581	8	29	theme	acid	1664:1667	arg1	contents					1669:1676	ascorbic acid contents	1655:1676	ascorbic acid contents	1655:1676	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	9	30	theme	shelf	1888:1892	arg1	life					1894:1897	the shelf life	1884:1897	the shelf life of tomato fruits	1884:1914	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	2	31	theme	peel	412:415	arg1	extract					430:436	an avocado pear peel polyphenolic extract	396:436	an avocado pear peel polyphenolic extract enriched keratin-starch composite film	396:475	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	6	32	theme	significant	1141:1151	arg1	effect					1153:1158	No significant effect	1138:1158	No significant effect	1138:1158	No significant effect was observed in catechol oxidase activity of the tomato extract across the different treatment groups.					
35288581	7	33	dep	showed	1301:1306	arg1	compared					1410:1417	compared	1410:1417	showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control	1301:1428	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	8	34	theme	polyphenolic	1474:1485	arg1	coating					1512:1518	polyphenolic activated keratin-starch coating	1474:1518	polyphenolic activated keratin-starch coating	1474:1518	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	8	35	theme	keratin-starch	1497:1510	arg1	coating					1512:1518	polyphenolic activated keratin-starch coating	1474:1518	polyphenolic activated keratin-starch coating	1474:1518	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	2	36	theme	antimicrobial	525:537	arg1	activity					539:546	antimicrobial activity	525:546	antimicrobial activity against fungal infected tomato fruits	525:584	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	0	37	theme	extract	72:78	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Fabrication, characterization and antifungal evaluation of polyphenolic extract activated keratin starch coating on infected tomato fruits.					
35288581	0	37	theme	extract	72:78	arg1	evaluation					45:54	antifungal evaluation	34:54	antifungal evaluation	34:54	Fabrication, characterization and antifungal evaluation of polyphenolic extract activated keratin starch coating on infected tomato fruits.					
35288581	0	37	theme	extract	72:78	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication, characterization and antifungal evaluation of polyphenolic extract activated keratin starch coating on infected tomato fruits.					
35288581	4	38	theme	increasing	876:885	arg1	concentration					895:907	increasing extract concentration	876:907	increasing extract concentration	876:907	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	2	39	theme	keratin-starch	447:460	arg1	film					472:475	keratin-starch composite film	447:475	keratin-starch composite film	447:475	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	0	40	theme	antifungal	34:43	arg1	evaluation					45:54	antifungal evaluation	34:54	antifungal evaluation	34:54	Fabrication, characterization and antifungal evaluation of polyphenolic extract activated keratin starch coating on infected tomato fruits.					
35288581	5	41	theme	pear	1093:1096	arg1	films					1131:1135	the avocado pear peel polyphenolic extract-loaded films	1081:1135	the avocado pear peel polyphenolic extract-loaded films	1081:1135	Ascorbic acid and lycopene contents were significantly (p < 0.05) higher in the avocado pear peel polyphenolic extract-loaded films.					
35288581	7	42	theme	coated	1389:1394	arg1	fruits					1403:1408	coated tomato fruits	1389:1408	coated tomato fruits	1389:1408	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	3	43	theme	film	696:699	arg1	formation					701:709	the successful film formation	681:709	the successful film formation with high degree of compatibility and homogeneity	681:759	The SEM/EDX and FTIR results revealed the successful film formation with high degree of compatibility and homogeneity.					
35288581	5	44	theme	polyphenolic	1103:1114	arg1	films					1131:1135	the avocado pear peel polyphenolic extract-loaded films	1081:1135	the avocado pear peel polyphenolic extract-loaded films	1081:1135	Ascorbic acid and lycopene contents were significantly (p < 0.05) higher in the avocado pear peel polyphenolic extract-loaded films.					
35288581	6	45	theme	tomato	1209:1214	arg1	extract					1216:1222	the tomato extract	1205:1222	the tomato extract	1205:1222	No significant effect was observed in catechol oxidase activity of the tomato extract across the different treatment groups.					
35288581	8	46	theme	fungal-infected	1682:1696	arg1	fruit					1705:1709	fungal-infected tomato fruit	1682:1709	fungal-infected tomato fruit	1682:1709	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	3	47	theme	compatibility	731:743	arg1	degree					721:726	high degree	716:726	high degree of compatibility and homogeneity	716:759	The SEM/EDX and FTIR results revealed the successful film formation with high degree of compatibility and homogeneity.					
35288581	7	48	theme	peel	1363:1366	arg1	load					1381:1384	avocado pear peel polyphenolic load	1350:1384	avocado pear peel polyphenolic load in coated tomato fruits	1350:1408	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	8	49	theme	fruit	1705:1709	arg1	quality					1599:1605	the overall quality	1587:1605	the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit	1587:1709	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	8	50	theme	spoilage-induce	1539:1553	arg1	loss					1562:1565	spoilage-induce weight loss	1539:1565	spoilage-induce weight loss	1539:1565	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	2	51	theme	avocado	399:405	arg1	extract					430:436	an avocado pear peel polyphenolic extract	396:436	an avocado pear peel polyphenolic extract enriched keratin-starch composite film	396:475	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	9	52	theme	polyphenolic	1750:1761	arg1	coating					1788:1794	polyphenolic activated keratin-starch coating	1750:1794	polyphenolic activated keratin-starch coating	1750:1794	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	9	52	theme	polyphenolic	1750:1761	arg1	method					1866:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	7	53	theme	avocado	1350:1356	arg1	load					1381:1384	avocado pear peel polyphenolic load	1350:1384	avocado pear peel polyphenolic load in coated tomato fruits	1350:1408	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	1	54	theme	products	270:277	arg1	preservation					232:243	postharvest preservation	220:243	postharvest preservation of food and agricultural products	220:277	In recent times, the application of protein-based bio-composite edible films in postharvest preservation of food and agricultural products is attracting increased attention due to their biodegradability, eco-friendliness and sustainability.					
35288581	9	55	theme	keratin-starch	1773:1786	arg1	coating					1788:1794	polyphenolic activated keratin-starch coating	1750:1794	polyphenolic activated keratin-starch coating	1750:1794	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	9	55	theme	keratin-starch	1773:1786	arg1	method					1866:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	7	56	theme	consistent	1334:1343	arg1	increase					1325:1332	a dose dependent increase	1308:1332	a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits	1308:1408	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	3	57	theme	homogeneity	749:759	arg1	degree					721:726	high degree	716:726	high degree of compatibility and homogeneity	716:759	The SEM/EDX and FTIR results revealed the successful film formation with high degree of compatibility and homogeneity.					
35288581	6	58	theme	extract	1216:1222	arg1	activity					1193:1200	catechol oxidase activity	1176:1200	catechol oxidase activity of the tomato extract across the different treatment groups	1176:1260	No significant effect was observed in catechol oxidase activity of the tomato extract across the different treatment groups.					
35288581	9	59	theme	tomato	1902:1907	arg1	fruits					1909:1914	tomato fruits	1902:1914	tomato fruits	1902:1914	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	1	60	theme	bio-composite	190:202	arg1	films					211:215	protein-based bio-composite edible films	176:215	protein-based bio-composite edible films	176:215	In recent times, the application of protein-based bio-composite edible films in postharvest preservation of food and agricultural products is attracting increased attention due to their biodegradability, eco-friendliness and sustainability.					
35288581	9	61	theme	sustainable	1813:1823	arg1	coating					1788:1794	polyphenolic activated keratin-starch coating	1750:1794	polyphenolic activated keratin-starch coating	1750:1794	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	9	61	theme	sustainable	1813:1823	arg1	method					1866:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	6	62	theme	different	1235:1243	arg1	groups					1255:1260	the different treatment groups	1231:1260	the different treatment groups	1231:1260	No significant effect was observed in catechol oxidase activity of the tomato extract across the different treatment groups.					
35288581	1	63	theme	films	211:215	arg1	application					161:171	the application	157:171	the application of protein-based bio-composite edible films in postharvest preservation of food and agricultural products	157:277	In recent times, the application of protein-based bio-composite edible films in postharvest preservation of food and agricultural products is attracting increased attention due to their biodegradability, eco-friendliness and sustainability.					
35288581	4	64	theme	extract	991:997	arg1	load					999:1002	extract load	991:1002	extract load	991:1002	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	3	65	theme	SEM/EDX	647:653	arg1	results					664:670	The SEM/EDX and FTIR results	643:670	The SEM/EDX and FTIR results	643:670	The SEM/EDX and FTIR results revealed the successful film formation with high degree of compatibility and homogeneity.					
35288581	1	66	theme	postharvest	220:230	arg1	preservation					232:243	postharvest preservation	220:243	postharvest preservation of food and agricultural products	220:277	In recent times, the application of protein-based bio-composite edible films in postharvest preservation of food and agricultural products is attracting increased attention due to their biodegradability, eco-friendliness and sustainability.					
35288581	9	67	theme	preservation	1853:1864	arg1	coating					1788:1794	polyphenolic activated keratin-starch coating	1750:1794	polyphenolic activated keratin-starch coating	1750:1794	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	9	67	theme	preservation	1853:1864	arg1	method					1866:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	5	68	dep	higher	1071:1076	arg1	p < 0.05					1061:1068	p < 0.05	1061:1068	p < 0.05	1061:1068	Ascorbic acid and lycopene contents were significantly (p < 0.05) higher in the avocado pear peel polyphenolic extract-loaded films.					
35288581	8	69	theme	overall	1591:1597	arg1	quality					1599:1605	the overall quality	1587:1605	the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit	1587:1709	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	4	70	theme	post-coating	780:791	arg1	loss					793:796	Following a 6-day post-coating loss	762:796	Following a 6-day post-coating loss in weight of the coated tomato fruits	762:834	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	3	71	theme	FTIR	659:662	arg1	results					664:670	The SEM/EDX and FTIR results	643:670	The SEM/EDX and FTIR results	643:670	The SEM/EDX and FTIR results revealed the successful film formation with high degree of compatibility and homogeneity.					
35288581	4	72	dep	decreased	836:844	arg1	p < 0.05					861:868	p < 0.05	861:868	p < 0.05	861:868	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	2	73	theme	fungal	556:561	arg1	fruits					579:584	fungal infected tomato fruits	556:584	fungal infected tomato fruits	556:584	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	0	74	theme	starch	98:103	arg1	coating					105:111	keratin starch coating	90:111	keratin starch coating on infected tomato fruits	90:137	Fabrication, characterization and antifungal evaluation of polyphenolic extract activated keratin starch coating on infected tomato fruits.					
35288581	9	75	theme	ecofriendly	1829:1839	arg1	coating					1788:1794	polyphenolic activated keratin-starch coating	1750:1794	polyphenolic activated keratin-starch coating	1750:1794	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	9	75	theme	ecofriendly	1829:1839	arg1	method					1866:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	8	76	theme	titratable	1618:1627	arg1	levels					1634:1639	titratable acid levels	1618:1639	titratable acid levels	1618:1639	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	7	77	theme	growth	1283:1288	arg1	inhibition					1290:1299	fungal growth inhibition	1276:1299	fungal growth inhibition	1276:1299	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	1	78	from	application	161:171	arg1	preservation					232:243	postharvest preservation	220:243	postharvest preservation of food and agricultural products	220:277	In recent times, the application of protein-based bio-composite edible films in postharvest preservation of food and agricultural products is attracting increased attention due to their biodegradability, eco-friendliness and sustainability.					
35288581	4	79	theme	coated	815:820	arg1	fruits					829:834	the coated tomato fruits	811:834	the coated tomato fruits	811:834	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	0	80	theme	tomato	125:130	arg1	fruits					132:137	infected tomato fruits	116:137	infected tomato fruits	116:137	Fabrication, characterization and antifungal evaluation of polyphenolic extract activated keratin starch coating on infected tomato fruits.					
35288581	2	81	theme	tomato	572:577	arg1	fruits					579:584	fungal infected tomato fruits	556:584	fungal infected tomato fruits	556:584	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	4	82	theme	fruits	829:834	arg1	weight					801:806	weight	801:806	weight of the coated tomato fruits	801:834	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	2	83	theme	polyphenolic	417:428	arg1	extract					430:436	an avocado pear peel polyphenolic extract	396:436	an avocado pear peel polyphenolic extract enriched keratin-starch composite film	396:475	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	5	84	theme	acid	1014:1017	arg1	contents					1032:1039	Ascorbic acid and lycopene contents	1005:1039	Ascorbic acid and lycopene contents	1005:1039	Ascorbic acid and lycopene contents were significantly (p < 0.05) higher in the avocado pear peel polyphenolic extract-loaded films.					
35288581	8	85	theme	acid	1629:1632	arg1	levels					1634:1639	titratable acid levels	1618:1639	titratable acid levels	1618:1639	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	9	86	theme	postharvest	1841:1851	arg1	coating					1788:1794	polyphenolic activated keratin-starch coating	1750:1794	polyphenolic activated keratin-starch coating	1750:1794	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	9	86	theme	postharvest	1841:1851	arg1	method					1866:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	a sustainable and ecofriendly postharvest preservation method	1811:1871	Therefore polyphenolic activated keratin-starch coating could serve as a sustainable and ecofriendly postharvest preservation method to prolong the shelf life of tomato fruits.					
35288581	8	87	theme	ascorbic	1655:1662	arg1	contents					1669:1676	ascorbic acid contents	1655:1676	ascorbic acid contents	1655:1676	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	2	88	theme	pear	407:410	arg1	extract					430:436	an avocado pear peel polyphenolic extract	396:436	an avocado pear peel polyphenolic extract enriched keratin-starch composite film	396:475	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	5	89	theme	lycopene	1023:1030	arg1	contents					1032:1039	Ascorbic acid and lycopene contents	1005:1039	Ascorbic acid and lycopene contents	1005:1039	Ascorbic acid and lycopene contents were significantly (p < 0.05) higher in the avocado pear peel polyphenolic extract-loaded films.					
35288581	7	90	theme	tomato	1396:1401	arg1	fruits					1403:1408	coated tomato fruits	1389:1408	coated tomato fruits	1389:1408	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	2	91	theme	composite	462:470	arg1	film					472:475	keratin-starch composite film	447:475	keratin-starch composite film	447:475	In this study, an avocado pear peel polyphenolic extract enriched keratin-starch composite film was fabricated, characterized and evaluated for antimicrobial activity against fungal infected tomato fruits after 6 days of storage at room (25 ± 2 °C) temperature.					
35288581	5	92	theme	avocado	1085:1091	arg1	films					1131:1135	the avocado pear peel polyphenolic extract-loaded films	1081:1135	the avocado pear peel polyphenolic extract-loaded films	1081:1135	Ascorbic acid and lycopene contents were significantly (p < 0.05) higher in the avocado pear peel polyphenolic extract-loaded films.					
35288581	3	93	theme	successful	685:694	arg1	formation					701:709	the successful film formation	681:709	the successful film formation with high degree of compatibility and homogeneity	681:759	The SEM/EDX and FTIR results revealed the successful film formation with high degree of compatibility and homogeneity.					
35288581	8	94	theme	activated	1487:1495	arg1	coating					1512:1518	polyphenolic activated keratin-starch coating	1474:1518	polyphenolic activated keratin-starch coating	1474:1518	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	7	95	theme	fungal	1276:1281	arg1	inhibition					1290:1299	fungal growth inhibition	1276:1299	fungal growth inhibition	1276:1299	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	0	96	theme	polyphenolic	59:70	arg1	extract					72:78	polyphenolic extract	59:78	polyphenolic extract	59:78	Fabrication, characterization and antifungal evaluation of polyphenolic extract activated keratin starch coating on infected tomato fruits.					
35288581	5	97	theme	peel	1098:1101	arg1	films					1131:1135	the avocado pear peel polyphenolic extract-loaded films	1081:1135	the avocado pear peel polyphenolic extract-loaded films	1081:1135	Ascorbic acid and lycopene contents were significantly (p < 0.05) higher in the avocado pear peel polyphenolic extract-loaded films.					
35288581	7	98	theme	polyphenolic	1368:1379	arg1	load					1381:1384	avocado pear peel polyphenolic load	1350:1384	avocado pear peel polyphenolic load in coated tomato fruits	1350:1408	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	8	99	theme	tomato	1698:1703	arg1	fruit					1705:1709	fungal-infected tomato fruit	1682:1709	fungal-infected tomato fruit	1682:1709	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	4	100	theme	extract	887:893	arg1	concentration					895:907	increasing extract concentration	876:907	increasing extract concentration	876:907	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	4	101	from	loss	793:796	arg1	weight					801:806	weight	801:806	weight of the coated tomato fruits	801:834	Following a 6-day post-coating loss in weight of the coated tomato fruits decreased significantly (p < 0.05) with increasing extract concentration while titratable acidity showed a significant (p < 0.05) increase with increasing extract load.					
35288581	7	102	theme	pear	1358:1361	arg1	load					1381:1384	avocado pear peel polyphenolic load	1350:1384	avocado pear peel polyphenolic load in coated tomato fruits	1350:1408	In addition, fungal growth inhibition showed a dose dependent increase consistent with avocado pear peel polyphenolic load in coated tomato fruits compared to control.					
35288581	3	103	theme	high	716:719	arg1	degree					721:726	high degree	716:726	high degree of compatibility and homogeneity	716:759	The SEM/EDX and FTIR results revealed the successful film formation with high degree of compatibility and homogeneity.					
35288581	6	104	theme	oxidase	1185:1191	arg1	activity					1193:1200	catechol oxidase activity	1176:1200	catechol oxidase activity of the tomato extract across the different treatment groups	1176:1260	No significant effect was observed in catechol oxidase activity of the tomato extract across the different treatment groups.					
35288581	1	105	theme	food	248:251	arg1	preservation					232:243	postharvest preservation	220:243	postharvest preservation of food and agricultural products	220:277	In recent times, the application of protein-based bio-composite edible films in postharvest preservation of food and agricultural products is attracting increased attention due to their biodegradability, eco-friendliness and sustainability.					
35288581	8	106	theme	microbial	1722:1730	arg1	growth					1732:1737	microbial growth	1722:1737	microbial growth	1722:1737	Results obtained in this study showed that polyphenolic activated keratin-starch coating was able to reduce spoilage-induce weight loss as well as conserve the overall quality (including titratable acid levels, lycopene and ascorbic acid contents) of fungal-infected tomato fruit and reduce microbial growth.					
35288581	1	107	theme	agricultural	257:268	arg1	products					270:277	agricultural products	257:277	agricultural products	257:277	In recent times, the application of protein-based bio-composite edible films in postharvest preservation of food and agricultural products is attracting increased attention due to their biodegradability, eco-friendliness and sustainability.					
36961583	11	0	theme	0.5 g/L	1653:1659	arg1	ZnC4H6O4					1661:1668	0.5 g/L ZnC4H6O4	1653:1668	0.5 g/L ZnC4H6O4	1653:1668	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	1	1	theme	main	162:165	arg1	causes					167:172	the main causes	158:172	the main causes of death worldwide	158:191	Cardiovascular complications due to thrombosis have become one of the main causes of death worldwide.					
36961583	11	2	theme	14.22 g/L	1569:1577	arg1	fructose					1579:1586	14.22 g/L fructose	1569:1586	14.22 g/L fructose	1569:1586	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	11	2	theme	14.22 g/L	1569:1577	arg1	composition					1469:1479	The medium composition	1458:1479	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology	1458:1563	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	4	3	theme	fermentative	634:645	arg1	sources					655:661	fermentative protein sources	634:661	fermentative protein sources	634:661	In this study, isolation, identification, and optimization of fibrinolytic protease enzyme-producing strains were performed using fermentative protein sources.					
36961583	5	4	theme	isolated	735:742	arg1	strains					744:750	the isolated strains	731:750	the isolated strains on skim milk agar medium	731:775	Fibrinolytic protease-producing strains were selected by analyzing the isolated strains on skim milk agar medium.					
36961583	6	5	theme	enzymatic	878:886	arg1	activity					888:895	high enzymatic activity	873:895	high enzymatic activity	873:895	The selected strains were examined on blood agar and fibrin plate medium, and the ones showing high enzymatic activity were determined.					
36961583	1	6	theme	causes	167:172	arg1	one					151:153	one	151:153	one	151:153	Cardiovascular complications due to thrombosis have become one of the main causes of death worldwide.					
36961583	1	6	theme	causes	167:172	arg1	causes					167:172	the main causes	158:172	the main causes of death worldwide	158:191	Cardiovascular complications due to thrombosis have become one of the main causes of death worldwide.					
36961583	11	7	theme	Surface	1545:1551	arg1	Methodology					1553:1563	Response Surface Methodology	1536:1563	Response Surface Methodology	1536:1563	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	10	8	theme	ZnC4H6O4	1394:1401	arg1	components					1403:1412	MnCl2 and ZnC4H6O4 components	1384:1412	MnCl2 and ZnC4H6O4 components	1384:1412	It was observed that CaCl2, KH2PO4, and MgSO4 components had a negative effect, while MnCl2 and ZnC4H6O4 components had a positive effect on enzyme production.					
36961583	3	9	theme	Fibrinolytic	383:394	arg1	candidates					477:486	potential therapeutic candidates	455:486	potential therapeutic candidates for thrombosis	455:501	Fibrinolytic enzymes, especially of microbial origin, are recognized as potential therapeutic candidates for thrombosis.					
36961583	3	9	theme	Fibrinolytic	383:394	arg1	enzymes					396:402	Fibrinolytic enzymes	383:402	Fibrinolytic enzymes	383:402	Fibrinolytic enzymes, especially of microbial origin, are recognized as potential therapeutic candidates for thrombosis.					
36961583	5	10	theme	Fibrinolytic	664:675	arg1	strains					696:702	Fibrinolytic protease-producing strains	664:702	Fibrinolytic protease-producing strains	664:702	Fibrinolytic protease-producing strains were selected by analyzing the isolated strains on skim milk agar medium.					
36961583	9	11	theme	different	1129:1137	arg1	conditions					1146:1155	different medium conditions	1129:1155	different medium conditions used for enzyme production	1129:1182	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	5	12	theme	protease-producing	677:694	arg1	strains					696:702	Fibrinolytic protease-producing strains	664:702	Fibrinolytic protease-producing strains	664:702	Fibrinolytic protease-producing strains were selected by analyzing the isolated strains on skim milk agar medium.					
36961583	9	13	theme	medium	1139:1144	arg1	conditions					1146:1155	different medium conditions	1129:1155	different medium conditions used for enzyme production	1129:1182	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	4	14	theme	protein	647:653	arg1	sources					655:661	fermentative protein sources	634:661	fermentative protein sources	634:661	In this study, isolation, identification, and optimization of fibrinolytic protease enzyme-producing strains were performed using fermentative protein sources.					
36961583	8	15	theme	protease	1059:1066	arg1	production					1068:1077	The maximum fibrinolytic protease production	1034:1077	The maximum fibrinolytic protease production of the strain	1034:1091	The maximum fibrinolytic protease production of the strain occurred at 60 °C and pH 7.0.					
36961583	6	16	theme	selected	782:789	arg1	strains					791:797	The selected strains	778:797	The selected strains	778:797	The selected strains were examined on blood agar and fibrin plate medium, and the ones showing high enzymatic activity were determined.					
36961583	3	17	theme	therapeutic	465:475	arg1	candidates					477:486	potential therapeutic candidates	455:486	potential therapeutic candidates for thrombosis	455:501	Fibrinolytic enzymes, especially of microbial origin, are recognized as potential therapeutic candidates for thrombosis.					
36961583	3	17	theme	therapeutic	465:475	arg1	enzymes					396:402	Fibrinolytic enzymes	383:402	Fibrinolytic enzymes	383:402	Fibrinolytic enzymes, especially of microbial origin, are recognized as potential therapeutic candidates for thrombosis.					
36961583	10	18	contain	had	1355:1357	arg1	KH2PO4					1326:1331	KH2PO4	1326:1331	KH2PO4	1326:1331	It was observed that CaCl2, KH2PO4, and MgSO4 components had a negative effect, while MnCl2 and ZnC4H6O4 components had a positive effect on enzyme production.					
36961583	10	18	contain	had	1355:1357	arg1	components					1344:1353	components	1344:1353	components	1344:1353	It was observed that CaCl2, KH2PO4, and MgSO4 components had a negative effect, while MnCl2 and ZnC4H6O4 components had a positive effect on enzyme production.					
36961583	10	18	contain	had	1355:1357	arg1	CaCl2					1319:1323	CaCl2	1319:1323	CaCl2	1319:1323	It was observed that CaCl2, KH2PO4, and MgSO4 components had a negative effect, while MnCl2 and ZnC4H6O4 components had a positive effect on enzyme production.					
36961583	10	18	contain	had	1355:1357	arg2	effect					1370:1375	a negative effect	1359:1375	a negative effect	1359:1375	It was observed that CaCl2, KH2PO4, and MgSO4 components had a negative effect, while MnCl2 and ZnC4H6O4 components had a positive effect on enzyme production.					
36961583	11	19	theme	14.22 g/L	1615:1623	arg1	peptone					1625:1631	14.22 g/L peptone	1615:1631	14.22 g/L peptone	1615:1631	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	11	20	theme	medium	1462:1467	arg1	fructose					1579:1586	14.22 g/L fructose	1569:1586	14.22 g/L fructose	1569:1586	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	11	20	theme	medium	1462:1467	arg1	composition					1469:1479	The medium composition	1458:1479	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology	1458:1563	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	1	21	theme	death	177:181	arg1	worldwide					183:191	death worldwide	177:191	death worldwide	177:191	Cardiovascular complications due to thrombosis have become one of the main causes of death worldwide.					
36961583	7	22	theme	rRNA	1019:1022	arg1	analysis					1024:1031	16S rRNA analysis	1015:1031	16S rRNA analysis	1015:1031	The strain determined to have the highest activity was identified as Acinetobacter johnsonii TR01 by 16S rRNA analysis.					
36961583	7	23	theme	16S	1015:1017	arg1	analysis					1024:1031	16S rRNA analysis	1015:1031	16S rRNA analysis	1015:1031	The strain determined to have the highest activity was identified as Acinetobacter johnsonii TR01 by 16S rRNA analysis.					
36961583	1	24	theme	Cardiovascular	92:105	arg1	complications					107:119	Cardiovascular complications	92:119	Cardiovascular complications due to thrombosis	92:137	Cardiovascular complications due to thrombosis have become one of the main causes of death worldwide.					
36961583	11	25	theme	0.5 g/L	1634:1640	arg1	MnCl2					1642:1646	0.5 g/L MnCl2	1634:1646	0.5 g/L MnCl2	1634:1646	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	1	26	theme	worldwide	183:191	arg1	causes					167:172	the main causes	158:172	the main causes of death worldwide	158:191	Cardiovascular complications due to thrombosis have become one of the main causes of death worldwide.					
36961583	3	27	theme	microbial	419:427	arg1	origin					429:434	microbial origin	419:434	microbial origin	419:434	Fibrinolytic enzymes, especially of microbial origin, are recognized as potential therapeutic candidates for thrombosis.					
36961583	4	28	theme	strains	605:611	arg1	identification					530:543	identification	530:543	identification	530:543	In this study, isolation, identification, and optimization of fibrinolytic protease enzyme-producing strains were performed using fermentative protein sources.					
36961583	4	28	theme	strains	605:611	arg1	optimization					550:561	optimization	550:561	optimization	550:561	In this study, isolation, identification, and optimization of fibrinolytic protease enzyme-producing strains were performed using fermentative protein sources.					
36961583	4	28	theme	strains	605:611	arg1	isolation					519:527	isolation	519:527	isolation	519:527	In this study, isolation, identification, and optimization of fibrinolytic protease enzyme-producing strains were performed using fermentative protein sources.					
36961583	7	29	theme	johnsonii	997:1005	arg1	strain					918:923	The strain	914:923	The strain determined to have the highest activity	914:963	The strain determined to have the highest activity was identified as Acinetobacter johnsonii TR01 by 16S rRNA analysis.					
36961583	7	29	theme	johnsonii	997:1005	arg1	TR01					1007:1010	Acinetobacter johnsonii TR01	983:1010	Acinetobacter johnsonii TR01	983:1010	The strain determined to have the highest activity was identified as Acinetobacter johnsonii TR01 by 16S rRNA analysis.					
36961583	9	30	theme	enzyme	1166:1171	arg1	production					1173:1182	enzyme production	1166:1182	enzyme production	1166:1182	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	3	31	theme	potential	455:463	arg1	candidates					477:486	potential therapeutic candidates	455:486	potential therapeutic candidates for thrombosis	455:501	Fibrinolytic enzymes, especially of microbial origin, are recognized as potential therapeutic candidates for thrombosis.					
36961583	3	31	theme	potential	455:463	arg1	enzymes					396:402	Fibrinolytic enzymes	383:402	Fibrinolytic enzymes	383:402	Fibrinolytic enzymes, especially of microbial origin, are recognized as potential therapeutic candidates for thrombosis.					
36961583	9	32	theme	yeast	1240:1244	arg1	sources					1289:1295	the best nitrogen sources	1271:1295	the best nitrogen sources	1271:1295	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	9	32	theme	yeast	1240:1244	arg1	peptone					1258:1264	peptone	1258:1264	peptone	1258:1264	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	9	32	theme	yeast	1240:1244	arg1	extract					1246:1252	yeast extract	1240:1252	yeast extract	1240:1252	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	4	33	theme	enzyme-producing	588:603	arg1	strains					605:611	fibrinolytic protease enzyme-producing strains	566:611	fibrinolytic protease enzyme-producing strains	566:611	In this study, isolation, identification, and optimization of fibrinolytic protease enzyme-producing strains were performed using fermentative protein sources.					
36961583	8	34	theme	strain	1086:1091	arg1	production					1068:1077	The maximum fibrinolytic protease production	1034:1077	The maximum fibrinolytic protease production of the strain	1034:1091	The maximum fibrinolytic protease production of the strain occurred at 60 °C and pH 7.0.					
36961583	1	35	theme	due	121:123	arg1	complications					107:119	Cardiovascular complications	92:119	Cardiovascular complications due to thrombosis	92:137	Cardiovascular complications due to thrombosis have become one of the main causes of death worldwide.					
36961583	8	36	theme	fibrinolytic	1046:1057	arg1	production					1068:1077	The maximum fibrinolytic protease production	1034:1077	The maximum fibrinolytic protease production of the strain	1034:1091	The maximum fibrinolytic protease production of the strain occurred at 60 °C and pH 7.0.					
36961583	10	37	theme	enzyme	1439:1444	arg1	production					1446:1455	enzyme production	1439:1455	enzyme production	1439:1455	It was observed that CaCl2, KH2PO4, and MgSO4 components had a negative effect, while MnCl2 and ZnC4H6O4 components had a positive effect on enzyme production.					
36961583	9	38	theme	best	1214:1217	arg1	fructose					1185:1192	fructose	1185:1192	fructose	1185:1192	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	9	38	theme	best	1214:1217	arg1	source					1226:1231	the best carbon source	1210:1231	the best carbon source	1210:1231	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	9	39	theme	best	1275:1278	arg1	sources					1289:1295	the best nitrogen sources	1271:1295	the best nitrogen sources	1271:1295	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	9	39	theme	best	1275:1278	arg1	peptone					1258:1264	peptone	1258:1264	peptone	1258:1264	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	9	39	theme	best	1275:1278	arg1	extract					1246:1252	yeast extract	1240:1252	yeast extract	1240:1252	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	2	40	theme	potential	301:309	arg1	strains					311:317	potential strains	301:317	potential strains that produce fibrinolytic enzymes for therapeutic applications	301:380	The high cost and undesirable side effects of existing thrombolytic agents have led researchers to isolate potential strains that produce fibrinolytic enzymes for therapeutic applications.					
36961583	11	41	theme	enzyme	1493:1498	arg1	activity					1500:1507	maximum enzyme activity	1485:1507	maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology	1485:1563	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	11	41	theme	enzyme	1493:1498	arg1	8.30 IU/ml					1510:1519	8.30 IU/ml	1510:1519	8.30 IU/ml	1510:1519	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	11	42	theme	maximum	1485:1491	arg1	activity					1500:1507	maximum enzyme activity	1485:1507	maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology	1485:1563	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	11	42	theme	maximum	1485:1491	arg1	8.30 IU/ml					1510:1519	8.30 IU/ml	1510:1519	8.30 IU/ml	1510:1519	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	9	43	theme	nitrogen	1280:1287	arg1	sources					1289:1295	the best nitrogen sources	1271:1295	the best nitrogen sources	1271:1295	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	9	43	theme	nitrogen	1280:1287	arg1	peptone					1258:1264	peptone	1258:1264	peptone	1258:1264	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	9	43	theme	nitrogen	1280:1287	arg1	extract					1246:1252	yeast extract	1240:1252	yeast extract	1240:1252	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	11	44	theme	yeast	1600:1604	arg1	extract					1606:1612	11.190 g/L yeast extract	1589:1612	11.190 g/L yeast extract	1589:1612	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	5	45	from	strains	744:750	arg1	medium					770:775	skim milk agar medium	755:775	skim milk agar medium	755:775	Fibrinolytic protease-producing strains were selected by analyzing the isolated strains on skim milk agar medium.					
36961583	11	46	theme	11.190 g/L	1589:1598	arg1	extract					1606:1612	11.190 g/L yeast extract	1589:1612	11.190 g/L yeast extract	1589:1612	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	6	47	theme	plate	838:842	arg1	medium					844:849	fibrin plate medium	831:849	fibrin plate medium	831:849	The selected strains were examined on blood agar and fibrin plate medium, and the ones showing high enzymatic activity were determined.					
36961583	10	48	contain	had	1414:1416	arg1	components					1403:1412	MnCl2 and ZnC4H6O4 components	1384:1412	MnCl2 and ZnC4H6O4 components	1384:1412	It was observed that CaCl2, KH2PO4, and MgSO4 components had a negative effect, while MnCl2 and ZnC4H6O4 components had a positive effect on enzyme production.					
36961583	10	48	contain	had	1414:1416	arg2	effect					1429:1434	a positive effect	1418:1434	a positive effect	1418:1434	It was observed that CaCl2, KH2PO4, and MgSO4 components had a negative effect, while MnCl2 and ZnC4H6O4 components had a positive effect on enzyme production.					
36961583	3	49	theme	origin	429:434	arg1	candidates					477:486	potential therapeutic candidates	455:486	potential therapeutic candidates for thrombosis	455:501	Fibrinolytic enzymes, especially of microbial origin, are recognized as potential therapeutic candidates for thrombosis.					
36961583	3	49	theme	origin	429:434	arg1	enzymes					396:402	Fibrinolytic enzymes	383:402	Fibrinolytic enzymes	383:402	Fibrinolytic enzymes, especially of microbial origin, are recognized as potential therapeutic candidates for thrombosis.					
36961583	5	50	theme	skim	755:758	arg1	milk					760:763	skim milk	755:763	skim milk agar medium	755:775	Fibrinolytic protease-producing strains were selected by analyzing the isolated strains on skim milk agar medium.					
36961583	2	51	theme	side	224:227	arg1	effects					229:235	undesirable side effects	212:235	undesirable side effects	212:235	The high cost and undesirable side effects of existing thrombolytic agents have led researchers to isolate potential strains that produce fibrinolytic enzymes for therapeutic applications.					
36961583	6	52	theme	fibrin	831:836	arg1	medium					844:849	fibrin plate medium	831:849	fibrin plate medium	831:849	The selected strains were examined on blood agar and fibrin plate medium, and the ones showing high enzymatic activity were determined.					
36961583	2	53	theme	therapeutic	357:367	arg1	applications					369:380	therapeutic applications	357:380	therapeutic applications	357:380	The high cost and undesirable side effects of existing thrombolytic agents have led researchers to isolate potential strains that produce fibrinolytic enzymes for therapeutic applications.					
36961583	4	54	theme	protease	579:586	arg1	strains					605:611	fibrinolytic protease enzyme-producing strains	566:611	fibrinolytic protease enzyme-producing strains	566:611	In this study, isolation, identification, and optimization of fibrinolytic protease enzyme-producing strains were performed using fermentative protein sources.					
36961583	5	55	theme	milk	760:763	arg1	medium					770:775	skim milk agar medium	755:775	skim milk agar medium	755:775	Fibrinolytic protease-producing strains were selected by analyzing the isolated strains on skim milk agar medium.					
36961583	2	56	theme	undesirable	212:222	arg1	effects					229:235	undesirable side effects	212:235	undesirable side effects	212:235	The high cost and undesirable side effects of existing thrombolytic agents have led researchers to isolate potential strains that produce fibrinolytic enzymes for therapeutic applications.					
36961583	4	57	theme	fibrinolytic	566:577	arg1	strains					605:611	fibrinolytic protease enzyme-producing strains	566:611	fibrinolytic protease enzyme-producing strains	566:611	In this study, isolation, identification, and optimization of fibrinolytic protease enzyme-producing strains were performed using fermentative protein sources.					
36961583	5	58	theme	agar	765:768	arg1	medium					770:775	skim milk agar medium	755:775	skim milk agar medium	755:775	Fibrinolytic protease-producing strains were selected by analyzing the isolated strains on skim milk agar medium.					
36961583	6	59	theme	high	873:876	arg1	activity					888:895	high enzymatic activity	873:895	high enzymatic activity	873:895	The selected strains were examined on blood agar and fibrin plate medium, and the ones showing high enzymatic activity were determined.					
36961583	7	60	theme	Acinetobacter	983:995	arg1	strain					918:923	The strain	914:923	The strain determined to have the highest activity	914:963	The strain determined to have the highest activity was identified as Acinetobacter johnsonii TR01 by 16S rRNA analysis.					
36961583	7	60	theme	Acinetobacter	983:995	arg1	TR01					1007:1010	Acinetobacter johnsonii TR01	983:1010	Acinetobacter johnsonii TR01	983:1010	The strain determined to have the highest activity was identified as Acinetobacter johnsonii TR01 by 16S rRNA analysis.					
36961583	10	61	theme	negative	1361:1368	arg1	effect					1370:1375	a negative effect	1359:1375	a negative effect	1359:1375	It was observed that CaCl2, KH2PO4, and MgSO4 components had a negative effect, while MnCl2 and ZnC4H6O4 components had a positive effect on enzyme production.					
36961583	2	62	theme	high	198:201	arg1	cost					203:206	high cost	198:206	high cost	198:206	The high cost and undesirable side effects of existing thrombolytic agents have led researchers to isolate potential strains that produce fibrinolytic enzymes for therapeutic applications.					
36961583	2	63	theme	agents	262:267	arg1	effects					229:235	undesirable side effects	212:235	undesirable side effects	212:235	The high cost and undesirable side effects of existing thrombolytic agents have led researchers to isolate potential strains that produce fibrinolytic enzymes for therapeutic applications.					
36961583	2	63	theme	agents	262:267	arg1	cost					203:206	high cost	198:206	high cost	198:206	The high cost and undesirable side effects of existing thrombolytic agents have led researchers to isolate potential strains that produce fibrinolytic enzymes for therapeutic applications.					
36961583	6	64	theme	blood	816:820	arg1	agar					822:825	blood agar	816:825	blood agar	816:825	The selected strains were examined on blood agar and fibrin plate medium, and the ones showing high enzymatic activity were determined.					
36961583	2	65	theme	fibrinolytic	332:343	arg1	enzymes					345:351	fibrinolytic enzymes	332:351	fibrinolytic enzymes for therapeutic applications	332:380	The high cost and undesirable side effects of existing thrombolytic agents have led researchers to isolate potential strains that produce fibrinolytic enzymes for therapeutic applications.					
36961583	0	66	theme	protease-producing	64:81	arg1	identification					11:24	identification	11:24	identification	11:24	Isolation, identification, and optimization of the fibrinolytic protease-producing strains.					
36961583	0	66	theme	protease-producing	64:81	arg1	optimization					31:42	optimization	31:42	optimization	31:42	Isolation, identification, and optimization of the fibrinolytic protease-producing strains.					
36961583	0	66	theme	protease-producing	64:81	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, identification, and optimization of the fibrinolytic protease-producing strains.					
36961583	2	67	theme	thrombolytic	249:260	arg1	agents					262:267	existing thrombolytic agents	240:267	existing thrombolytic agents	240:267	The high cost and undesirable side effects of existing thrombolytic agents have led researchers to isolate potential strains that produce fibrinolytic enzymes for therapeutic applications.					
36961583	8	68	theme	maximum	1038:1044	arg1	production					1068:1077	The maximum fibrinolytic protease production	1034:1077	The maximum fibrinolytic protease production of the strain	1034:1091	The maximum fibrinolytic protease production of the strain occurred at 60 °C and pH 7.0.					
36961583	10	69	theme	positive	1420:1427	arg1	effect					1429:1434	a positive effect	1418:1434	a positive effect	1418:1434	It was observed that CaCl2, KH2PO4, and MgSO4 components had a negative effect, while MnCl2 and ZnC4H6O4 components had a positive effect on enzyme production.					
36961583	11	70	theme	Response	1536:1543	arg1	Methodology					1553:1563	Response Surface Methodology	1536:1563	Response Surface Methodology	1536:1563	The medium composition for maximum enzyme activity (8.30 IU/ml) determined by Response Surface Methodology was 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.					
36961583	0	71	theme	fibrinolytic	51:62	arg1	protease-producing					64:81	the fibrinolytic protease-producing	47:81	the fibrinolytic protease-producing	47:81	Isolation, identification, and optimization of the fibrinolytic protease-producing strains.					
36961583	2	72	theme	existing	240:247	arg1	agents					262:267	existing thrombolytic agents	240:267	existing thrombolytic agents	240:267	The high cost and undesirable side effects of existing thrombolytic agents have led researchers to isolate potential strains that produce fibrinolytic enzymes for therapeutic applications.					
36961583	9	73	theme	carbon	1219:1224	arg1	fructose					1185:1192	fructose	1185:1192	fructose	1185:1192	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	9	73	theme	carbon	1219:1224	arg1	source					1226:1231	the best carbon source	1210:1231	the best carbon source	1210:1231	Under different medium conditions used for enzyme production, fructose was found to be the best carbon source, while yeast extract and peptone were the best nitrogen sources.					
36961583	7	74	theme	highest	948:954	arg1	activity					956:963	the highest activity	944:963	the highest activity	944:963	The strain determined to have the highest activity was identified as Acinetobacter johnsonii TR01 by 16S rRNA analysis.					
36961583	2	75	dep	cost	203:206	arg1	The					194:196	The	194:196	The	194:196	The high cost and undesirable side effects of existing thrombolytic agents have led researchers to isolate potential strains that produce fibrinolytic enzymes for therapeutic applications.					
35697869	6	0	theme	metabolomic	1337:1347	arg1	analysis					1349:1356	a non-targeted metabolomic analysis	1322:1356	a non-targeted metabolomic analysis	1322:1356	To this aim, we studied classical PTI responses such as the generation of ROS and MAPK phosphorylation, but we also monitored the biosynthesis of phytocytokines and performed a non-targeted metabolomic analysis.					
35697869	2	1	theme	exogenous	384:392	arg1	Patterns					502:509	Pathogen-Associated Molecular Patterns	472:509	Pathogen-Associated Molecular Patterns (PAMPs)	472:517	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	2	1	theme	exogenous	384:392	arg1	Patterns					452:459	Damage-Associated Molecular Patterns	424:459	Damage-Associated Molecular Patterns (DAMPs)	424:467	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	2	1	theme	exogenous	384:392	arg1	signals					408:414	endogenous or exogenous extracellular signals	370:414	endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs)	370:517	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	6	2	theme	MAPK	1229:1232	arg1	phosphorylation					1234:1248	MAPK phosphorylation	1229:1248	MAPK phosphorylation	1229:1248	To this aim, we studied classical PTI responses such as the generation of ROS and MAPK phosphorylation, but we also monitored the biosynthesis of phytocytokines and performed a non-targeted metabolomic analysis.					
35697869	2	3	theme	Plant	314:318	arg1	cells					320:324	Abstract Plant cells	305:324	Abstract Plant cells	305:324	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	1	4	theme	signal	297:302	arg1	origin					283:288	the origin	279:288	the origin of the signal	279:302	The simultaneous perception of endogenous and exogenous danger signals potentiates PAMP-triggered immunity in tomato and other downstream defence responses depending on the origin of the signal.					
35697869	6	5	theme	ROS	1221:1223	arg1	phosphorylation					1234:1248	MAPK phosphorylation	1229:1248	MAPK phosphorylation	1229:1248	To this aim, we studied classical PTI responses such as the generation of ROS and MAPK phosphorylation, but we also monitored the biosynthesis of phytocytokines and performed a non-targeted metabolomic analysis.					
35697869	6	5	theme	ROS	1221:1223	arg1	generation					1207:1216	the generation	1203:1216	the generation of ROS	1203:1223	To this aim, we studied classical PTI responses such as the generation of ROS and MAPK phosphorylation, but we also monitored the biosynthesis of phytocytokines and performed a non-targeted metabolomic analysis.					
35697869	2	6	theme	Damage-Associated	424:440	arg1	DAMPs					462:466	DAMPs	462:466	DAMPs	462:466	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	2	6	theme	Damage-Associated	424:440	arg1	Patterns					452:459	Damage-Associated Molecular Patterns	424:459	Damage-Associated Molecular Patterns (DAMPs)	424:467	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	7	7	theme	PAMP-triggered	1479:1492	arg1	responses					1503:1511	PAMP-triggered immunity responses	1479:1511	PAMP-triggered immunity responses	1479:1511	We demonstrate that co-application of the bacterial peptide flagellin with the DAMPs cyclic AMP or cellobiose amplifies PAMP-triggered immunity responses.					
35697869	2	8	theme	pathogen	337:344	arg1	invasion					346:353	a pathogen invasion	335:353	a pathogen invasion	335:353	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	4	9	theme	overlapped	988:997	arg1	responses					1015:1023	overlapped innate immunity responses	988:1023	overlapped innate immunity responses	988:1023	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	9	10	dep	bacteria	1770:1777	arg1	pv					1800:1801	Pseudomonas syringae pv	1779:1801	the hemibiotrophic bacteria Pseudomonas syringae pv	1751:1801	In addition, cAMP and cellobiose treatments induced resistance against the hemibiotrophic bacteria Pseudomonas syringae pv.					
35697869	11	11	theme	plant	1941:1945	arg1	responses					1955:1963	plant defence responses	1941:1963	plant defence responses	1941:1963	Overall, these results indicate that the complex mixture of DAMPs and PAMPs carries specific information that potentiates plant defence responses.					
35697869	5	12	theme	defence	1128:1134	arg1	responses					1136:1144	early and late plant defence responses	1107:1144	early and late plant defence responses	1107:1144	Here, we report that the simultaneous perception of DAMPs and a PAMP strengthens early and late plant defence responses.					
35697869	3	13	theme	released	612:619	arg1	DAMPs					535:539	DAMPs	535:539	DAMPs	535:539	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	3	13	theme	released	612:619	arg1	molecules					559:567	intracellular molecules	545:567	intracellular molecules	545:567	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	1	14	theme	other	231:235	arg1	responses					256:264	other downstream defence responses	231:264	other downstream defence responses	231:264	The simultaneous perception of endogenous and exogenous danger signals potentiates PAMP-triggered immunity in tomato and other downstream defence responses depending on the origin of the signal.					
35697869	4	15	theme	immunity	1006:1013	arg1	responses					1015:1023	overlapped innate immunity responses	988:1023	overlapped innate immunity responses	988:1023	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	3	16	theme	extracellular	644:656	arg1	recognition					658:668	recognition	658:668	recognition	658:668	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	1	17	theme	defence	248:254	arg1	responses					256:264	other downstream defence responses	231:264	other downstream defence responses	231:264	The simultaneous perception of endogenous and exogenous danger signals potentiates PAMP-triggered immunity in tomato and other downstream defence responses depending on the origin of the signal.					
35697869	4	18	theme	different	961:969	arg1	origins					971:977	such different origins	956:977	such different origins	956:977	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	7	19	theme	DAMPs	1438:1442	arg1	AMP					1451:1453	the DAMPs cyclic AMP	1434:1453	the DAMPs cyclic AMP	1434:1453	We demonstrate that co-application of the bacterial peptide flagellin with the DAMPs cyclic AMP or cellobiose amplifies PAMP-triggered immunity responses.					
35697869	5	20	theme	early	1107:1111	arg1	responses					1136:1144	early and late plant defence responses	1107:1144	early and late plant defence responses	1107:1144	Here, we report that the simultaneous perception of DAMPs and a PAMP strengthens early and late plant defence responses.					
35697869	8	21	theme	phytocytokines	1561:1574	arg1	synthesis					1548:1556	the synthesis	1544:1556	the synthesis of phytocytokines	1544:1574	Both co-applications enhanced the synthesis of phytocytokines, but only simultaneous treatments with cAMP strengthened the flagellin-dependent metabolomic responses.					
35697869	11	22	theme	PAMPs	1889:1893	arg1	mixture					1868:1874	the complex mixture	1856:1874	the complex mixture of DAMPs and PAMPs	1856:1893	Overall, these results indicate that the complex mixture of DAMPs and PAMPs carries specific information that potentiates plant defence responses.					
35697869	5	23	theme	late	1117:1120	arg1	responses					1136:1144	early and late plant defence responses	1107:1144	early and late plant defence responses	1107:1144	Here, we report that the simultaneous perception of DAMPs and a PAMP strengthens early and late plant defence responses.					
35697869	7	24	theme	peptide	1411:1417	arg1	flagellin					1419:1427	the bacterial peptide flagellin	1397:1427	the bacterial peptide flagellin	1397:1427	We demonstrate that co-application of the bacterial peptide flagellin with the DAMPs cyclic AMP or cellobiose amplifies PAMP-triggered immunity responses.					
35697869	2	25	theme	extracellular	394:406	arg1	Patterns					502:509	Pathogen-Associated Molecular Patterns	472:509	Pathogen-Associated Molecular Patterns (PAMPs)	472:517	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	2	25	theme	extracellular	394:406	arg1	Patterns					452:459	Damage-Associated Molecular Patterns	424:459	Damage-Associated Molecular Patterns (DAMPs)	424:467	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	2	25	theme	extracellular	394:406	arg1	signals					408:414	endogenous or exogenous extracellular signals	370:414	endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs)	370:517	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	11	26	theme	DAMPs	1879:1883	arg1	mixture					1868:1874	the complex mixture	1856:1874	the complex mixture of DAMPs and PAMPs	1856:1893	Overall, these results indicate that the complex mixture of DAMPs and PAMPs carries specific information that potentiates plant defence responses.					
35697869	1	27	theme	danger	166:171	arg1	signals					173:179	endogenous and exogenous danger signals	141:179	endogenous and exogenous danger signals	141:179	The simultaneous perception of endogenous and exogenous danger signals potentiates PAMP-triggered immunity in tomato and other downstream defence responses depending on the origin of the signal.					
35697869	8	28	theme	simultaneous	1586:1597	arg1	treatments					1599:1608	only simultaneous treatments	1581:1608	only simultaneous treatments with cAMP	1581:1618	Both co-applications enhanced the synthesis of phytocytokines, but only simultaneous treatments with cAMP strengthened the flagellin-dependent metabolomic responses.					
35697869	4	29	theme	extracellular	766:778	arg1	recognition					780:790	The extracellular recognition	762:790	The extracellular recognition of DAMPs and PAMPs	762:809	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	3	30	from	fragments	582:590	arg1	particular					523:532	particular	523:532	particular	523:532	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	0	31	theme	innate	83:88	arg1	responses					99:107	plant innate immunity responses	77:107	plant innate immunity responses	77:107	The simultaneous perception of self- and non-self-danger signals potentiates plant innate immunity responses.					
35697869	4	32	theme	similar	827:833	arg1	signalling					849:858	a very similar intracellular signalling	820:858	a very similar intracellular signalling	820:858	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	1	33	theme	simultaneous	114:125	arg1	perception					127:136	The simultaneous perception	110:136	The simultaneous perception of endogenous and exogenous danger signals	110:179	The simultaneous perception of endogenous and exogenous danger signals potentiates PAMP-triggered immunity in tomato and other downstream defence responses depending on the origin of the signal.					
35697869	11	34	theme	specific	1903:1910	arg1	information					1912:1922	specific information	1903:1922	specific information that potentiates plant defence responses	1903:1963	Overall, these results indicate that the complex mixture of DAMPs and PAMPs carries specific information that potentiates plant defence responses.					
35697869	7	35	theme	bacterial	1401:1409	arg1	flagellin					1419:1427	the bacterial peptide flagellin	1397:1427	the bacterial peptide flagellin	1397:1427	We demonstrate that co-application of the bacterial peptide flagellin with the DAMPs cyclic AMP or cellobiose amplifies PAMP-triggered immunity responses.					
35697869	8	36	theme	flagellin-dependent	1637:1655	arg1	responses					1669:1677	the flagellin-dependent metabolomic responses	1633:1677	the flagellin-dependent metabolomic responses	1633:1677	Both co-applications enhanced the synthesis of phytocytokines, but only simultaneous treatments with cAMP strengthened the flagellin-dependent metabolomic responses.					
35697869	8	37	with	treatments	1599:1608	arg1	cAMP					1615:1618	cAMP	1615:1618	cAMP	1615:1618	Both co-applications enhanced the synthesis of phytocytokines, but only simultaneous treatments with cAMP strengthened the flagellin-dependent metabolomic responses.					
35697869	9	38	theme	cAMP	1693:1696	arg1	treatments					1713:1722	cAMP and cellobiose treatments	1693:1722	cAMP and cellobiose treatments	1693:1722	In addition, cAMP and cellobiose treatments induced resistance against the hemibiotrophic bacteria Pseudomonas syringae pv.					
35697869	2	39	theme	Molecular	492:500	arg1	Patterns					502:509	Pathogen-Associated Molecular Patterns	472:509	Pathogen-Associated Molecular Patterns (PAMPs)	472:517	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	2	39	theme	Molecular	492:500	arg1	PAMPs					512:516	PAMPs	512:516	PAMPs	512:516	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	3	40	theme	wall	577:580	arg1	fragments					582:590	cell wall fragments	572:590	cell wall fragments	572:590	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	9	41	theme	cellobiose	1702:1711	arg1	treatments					1713:1722	cAMP and cellobiose treatments	1693:1722	cAMP and cellobiose treatments	1693:1722	In addition, cAMP and cellobiose treatments induced resistance against the hemibiotrophic bacteria Pseudomonas syringae pv.					
35697869	0	42	theme	self-	31:35	arg1	signals					57:63	self- and non-self-danger signals	31:63	self- and non-self-danger signals	31:63	The simultaneous perception of self- and non-self-danger signals potentiates plant innate immunity responses.					
35697869	3	43	theme	specific	695:702	arg1	immunity					752:759	the so-called DAMP-triggered immunity	723:759	the so-called DAMP-triggered immunity	723:759	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	3	43	theme	specific	695:702	arg1	signalling					711:720	specific immune signalling	695:720	specific immune signalling	695:720	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	4	44	theme	biological	897:906	arg1	need					908:911	a biological need	895:911	a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses	895:1023	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	0	45	theme	non-self-danger	41:55	arg1	signals					57:63	self- and non-self-danger signals	31:63	self- and non-self-danger signals	31:63	The simultaneous perception of self- and non-self-danger signals potentiates plant innate immunity responses.					
35697869	6	46	theme	PTI	1181:1183	arg1	phosphorylation					1234:1248	MAPK phosphorylation	1229:1248	MAPK phosphorylation	1229:1248	To this aim, we studied classical PTI responses such as the generation of ROS and MAPK phosphorylation, but we also monitored the biosynthesis of phytocytokines and performed a non-targeted metabolomic analysis.					
35697869	6	46	theme	PTI	1181:1183	arg1	generation					1207:1216	the generation	1203:1216	the generation of ROS	1203:1223	To this aim, we studied classical PTI responses such as the generation of ROS and MAPK phosphorylation, but we also monitored the biosynthesis of phytocytokines and performed a non-targeted metabolomic analysis.					
35697869	6	46	theme	PTI	1181:1183	arg1	responses					1185:1193	classical PTI responses	1171:1193	classical PTI responses such as the generation of ROS and MAPK phosphorylation	1171:1248	To this aim, we studied classical PTI responses such as the generation of ROS and MAPK phosphorylation, but we also monitored the biosynthesis of phytocytokines and performed a non-targeted metabolomic analysis.					
35697869	3	47	theme	DAMP-triggered	737:750	arg1	immunity					752:759	the so-called DAMP-triggered immunity	723:759	the so-called DAMP-triggered immunity	723:759	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	3	47	theme	DAMP-triggered	737:750	arg1	signalling					711:720	specific immune signalling	695:720	specific immune signalling	695:720	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	9	48	theme	hemibiotrophic	1755:1768	arg1	bacteria					1770:1777	the hemibiotrophic bacteria Pseudomonas syringae pv	1751:1801	the hemibiotrophic bacteria Pseudomonas syringae pv	1751:1801	In addition, cAMP and cellobiose treatments induced resistance against the hemibiotrophic bacteria Pseudomonas syringae pv.					
35697869	11	49	theme	complex	1860:1866	arg1	mixture					1868:1874	the complex mixture	1856:1874	the complex mixture of DAMPs and PAMPs	1856:1893	Overall, these results indicate that the complex mixture of DAMPs and PAMPs carries specific information that potentiates plant defence responses.					
35697869	9	50	theme	Pseudomonas	1779:1789	arg1	pv					1800:1801	Pseudomonas syringae pv	1779:1801	the hemibiotrophic bacteria Pseudomonas syringae pv	1751:1801	In addition, cAMP and cellobiose treatments induced resistance against the hemibiotrophic bacteria Pseudomonas syringae pv.					
35697869	3	51	theme	intracellular	545:557	arg1	DAMPs					535:539	DAMPs	535:539	DAMPs	535:539	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	3	51	theme	intracellular	545:557	arg1	molecules					559:567	intracellular molecules	545:567	intracellular molecules	545:567	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	6	52	theme	non-targeted	1324:1335	arg1	analysis					1349:1356	a non-targeted metabolomic analysis	1322:1356	a non-targeted metabolomic analysis	1322:1356	To this aim, we studied classical PTI responses such as the generation of ROS and MAPK phosphorylation, but we also monitored the biosynthesis of phytocytokines and performed a non-targeted metabolomic analysis.					
35697869	3	53	from	molecules	559:567	arg1	particular					523:532	particular	523:532	particular	523:532	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	3	54	theme	passive	592:598	arg1	DAMPs					535:539	DAMPs	535:539	DAMPs	535:539	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	3	54	theme	passive	592:598	arg1	molecules					559:567	intracellular molecules	545:567	intracellular molecules	545:567	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	2	55	theme	Abstract	305:312	arg1	cells					320:324	Abstract Plant cells	305:324	Abstract Plant cells	305:324	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	1	56	theme	PAMP-triggered	193:206	arg1	immunity					208:215	PAMP-triggered immunity	193:215	PAMP-triggered immunity in tomato	193:225	The simultaneous perception of endogenous and exogenous danger signals potentiates PAMP-triggered immunity in tomato and other downstream defence responses depending on the origin of the signal.					
35697869	6	57	theme	phytocytokines	1293:1306	arg1	biosynthesis					1277:1288	the biosynthesis	1273:1288	the biosynthesis of phytocytokines	1273:1306	To this aim, we studied classical PTI responses such as the generation of ROS and MAPK phosphorylation, but we also monitored the biosynthesis of phytocytokines and performed a non-targeted metabolomic analysis.					
35697869	11	58	contain	carries	1895:1901	arg2	information					1912:1922	specific information	1903:1922	specific information that potentiates plant defence responses	1903:1963	Overall, these results indicate that the complex mixture of DAMPs and PAMPs carries specific information that potentiates plant defence responses.					
35697869	11	58	contain	carries	1895:1901	arg1	mixture					1868:1874	the complex mixture	1856:1874	the complex mixture of DAMPs and PAMPs	1856:1893	Overall, these results indicate that the complex mixture of DAMPs and PAMPs carries specific information that potentiates plant defence responses.					
35697869	7	59	theme	immunity	1494:1501	arg1	responses					1503:1511	PAMP-triggered immunity responses	1479:1511	PAMP-triggered immunity responses	1479:1511	We demonstrate that co-application of the bacterial peptide flagellin with the DAMPs cyclic AMP or cellobiose amplifies PAMP-triggered immunity responses.					
35697869	9	60	dep	Pseudomonas	1779:1789	arg1	syringae					1791:1798	syringae	1791:1798	syringae	1791:1798	In addition, cAMP and cellobiose treatments induced resistance against the hemibiotrophic bacteria Pseudomonas syringae pv.					
35697869	11	61	theme	defence	1947:1953	arg1	responses					1955:1963	plant defence responses	1941:1963	plant defence responses	1941:1963	Overall, these results indicate that the complex mixture of DAMPs and PAMPs carries specific information that potentiates plant defence responses.					
35697869	1	62	theme	downstream	237:246	arg1	responses					256:264	other downstream defence responses	231:264	other downstream defence responses	231:264	The simultaneous perception of endogenous and exogenous danger signals potentiates PAMP-triggered immunity in tomato and other downstream defence responses depending on the origin of the signal.					
35697869	4	63	theme	innate	999:1004	arg1	responses					1015:1023	overlapped innate immunity responses	988:1023	overlapped innate immunity responses	988:1023	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	7	64	with	co-application	1379:1392	arg1	cellobiose					1458:1467	cellobiose	1458:1467	cellobiose	1458:1467	We demonstrate that co-application of the bacterial peptide flagellin with the DAMPs cyclic AMP or cellobiose amplifies PAMP-triggered immunity responses.					
35697869	7	64	with	co-application	1379:1392	arg1	AMP					1451:1453	the DAMPs cyclic AMP	1434:1453	the DAMPs cyclic AMP	1434:1453	We demonstrate that co-application of the bacterial peptide flagellin with the DAMPs cyclic AMP or cellobiose amplifies PAMP-triggered immunity responses.					
35697869	4	65	theme	such	956:959	arg1	origins					971:977	such different origins	956:977	such different origins	956:977	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	7	66	theme	cyclic	1444:1449	arg1	AMP					1451:1453	the DAMPs cyclic AMP	1434:1453	the DAMPs cyclic AMP	1434:1453	We demonstrate that co-application of the bacterial peptide flagellin with the DAMPs cyclic AMP or cellobiose amplifies PAMP-triggered immunity responses.					
35697869	1	67	from	responses	256:264	arg1	tomato					220:225	tomato	220:225	tomato	220:225	The simultaneous perception of endogenous and exogenous danger signals potentiates PAMP-triggered immunity in tomato and other downstream defence responses depending on the origin of the signal.					
35697869	3	68	theme	intact	673:678	arg1	cells					680:684	intact cells	673:684	intact cells	673:684	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	1	69	theme	endogenous	141:150	arg1	signals					173:179	endogenous and exogenous danger signals	141:179	endogenous and exogenous danger signals	141:179	The simultaneous perception of endogenous and exogenous danger signals potentiates PAMP-triggered immunity in tomato and other downstream defence responses depending on the origin of the signal.					
35697869	4	70	with	molecules	941:949	arg1	origins					971:977	such different origins	956:977	such different origins	956:977	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	4	70	with	molecules	941:949	arg1	responses					1015:1023	overlapped innate immunity responses	988:1023	overlapped innate immunity responses	988:1023	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	4	71	theme	DAMPs	795:799	arg1	recognition					780:790	The extracellular recognition	762:790	The extracellular recognition of DAMPs and PAMPs	762:809	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	0	72	theme	plant	77:81	arg1	responses					99:107	plant innate immunity responses	77:107	plant innate immunity responses	77:107	The simultaneous perception of self- and non-self-danger signals potentiates plant innate immunity responses.					
35697869	5	73	theme	plant	1122:1126	arg1	responses					1136:1144	early and late plant defence responses	1107:1144	early and late plant defence responses	1107:1144	Here, we report that the simultaneous perception of DAMPs and a PAMP strengthens early and late plant defence responses.					
35697869	1	74	theme	exogenous	156:164	arg1	signals					173:179	endogenous and exogenous danger signals	141:179	endogenous and exogenous danger signals	141:179	The simultaneous perception of endogenous and exogenous danger signals potentiates PAMP-triggered immunity in tomato and other downstream defence responses depending on the origin of the signal.					
35697869	7	75	theme	flagellin	1419:1427	arg1	co-application					1379:1392	co-application	1379:1392	co-application of the bacterial peptide flagellin with the DAMPs cyclic AMP or cellobiose	1379:1467	We demonstrate that co-application of the bacterial peptide flagellin with the DAMPs cyclic AMP or cellobiose amplifies PAMP-triggered immunity responses.					
35697869	0	76	theme	immunity	90:97	arg1	responses					99:107	plant innate immunity responses	77:107	plant innate immunity responses	77:107	The simultaneous perception of self- and non-self-danger signals potentiates plant innate immunity responses.					
35697869	1	77	from	immunity	208:215	arg1	tomato					220:225	tomato	220:225	tomato	220:225	The simultaneous perception of endogenous and exogenous danger signals potentiates PAMP-triggered immunity in tomato and other downstream defence responses depending on the origin of the signal.					
35697869	1	78	theme	signals	173:179	arg1	perception					127:136	The simultaneous perception	110:136	The simultaneous perception of endogenous and exogenous danger signals	110:179	The simultaneous perception of endogenous and exogenous danger signals potentiates PAMP-triggered immunity in tomato and other downstream defence responses depending on the origin of the signal.					
35697869	12	79	theme	mixture	2051:2057	arg1	composition					2032:2042	the composition	2028:2042	the composition of the mixture	2028:2057	However, downstream responses seem more specific depending on the composition of the mixture.					
35697869	8	80	theme	metabolomic	1657:1667	arg1	responses					1669:1677	the flagellin-dependent metabolomic responses	1633:1677	the flagellin-dependent metabolomic responses	1633:1677	Both co-applications enhanced the synthesis of phytocytokines, but only simultaneous treatments with cAMP strengthened the flagellin-dependent metabolomic responses.					
35697869	4	81	theme	intracellular	835:847	arg1	signalling					849:858	a very similar intracellular signalling	820:858	a very similar intracellular signalling	820:858	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	2	82	theme	Molecular	442:450	arg1	DAMPs					462:466	DAMPs	462:466	DAMPs	462:466	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	2	82	theme	Molecular	442:450	arg1	Patterns					452:459	Damage-Associated Molecular Patterns	424:459	Damage-Associated Molecular Patterns (DAMPs)	424:467	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	0	83	theme	simultaneous	4:15	arg1	perception					17:26	The simultaneous perception	0:26	The simultaneous perception of self- and non-self-danger signals	0:63	The simultaneous perception of self- and non-self-danger signals potentiates plant innate immunity responses.					
35697869	4	84	theme	PAMPs	805:809	arg1	recognition					780:790	The extracellular recognition	762:790	The extracellular recognition of DAMPs and PAMPs	762:809	The extracellular recognition of DAMPs and PAMPs leads to a very similar intracellular signalling, and this similarity has generated a biological need to know why plants perceive molecules with such different origins and with overlapped innate immunity responses.					
35697869	2	85	theme	Pathogen-Associated	472:490	arg1	Patterns					502:509	Pathogen-Associated Molecular Patterns	472:509	Pathogen-Associated Molecular Patterns (PAMPs)	472:517	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	2	85	theme	Pathogen-Associated	472:490	arg1	PAMPs					512:516	PAMPs	512:516	PAMPs	512:516	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	3	86	theme	immune	704:709	arg1	immunity					752:759	the so-called DAMP-triggered immunity	723:759	the so-called DAMP-triggered immunity	723:759	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	3	86	theme	immune	704:709	arg1	signalling					711:720	specific immune signalling	695:720	specific immune signalling	695:720	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	0	87	theme	signals	57:63	arg1	perception					17:26	The simultaneous perception	0:26	The simultaneous perception of self- and non-self-danger signals	0:63	The simultaneous perception of self- and non-self-danger signals potentiates plant innate immunity responses.					
35697869	5	88	theme	PAMP	1090:1093	arg1	perception					1064:1073	the simultaneous perception	1047:1073	the simultaneous perception of DAMPs and a PAMP	1047:1093	Here, we report that the simultaneous perception of DAMPs and a PAMP strengthens early and late plant defence responses.					
35697869	3	89	theme	so-called	727:735	arg1	immunity					752:759	the so-called DAMP-triggered immunity	723:759	the so-called DAMP-triggered immunity	723:759	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	3	89	theme	so-called	727:735	arg1	signalling					711:720	specific immune signalling	695:720	specific immune signalling	695:720	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
35697869	2	90	theme	endogenous	370:379	arg1	Patterns					502:509	Pathogen-Associated Molecular Patterns	472:509	Pathogen-Associated Molecular Patterns (PAMPs)	472:517	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	2	90	theme	endogenous	370:379	arg1	Patterns					452:459	Damage-Associated Molecular Patterns	424:459	Damage-Associated Molecular Patterns (DAMPs)	424:467	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	2	90	theme	endogenous	370:379	arg1	signals					408:414	endogenous or exogenous extracellular signals	370:414	endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs)	370:517	Abstract Plant cells perceive a pathogen invasion by recognising endogenous or exogenous extracellular signals such as Damage-Associated Molecular Patterns (DAMPs) or Pathogen-Associated Molecular Patterns (PAMPs).					
35697869	5	91	theme	DAMPs	1078:1082	arg1	perception					1064:1073	the simultaneous perception	1047:1073	the simultaneous perception of DAMPs and a PAMP	1047:1093	Here, we report that the simultaneous perception of DAMPs and a PAMP strengthens early and late plant defence responses.					
35697869	5	92	theme	simultaneous	1051:1062	arg1	perception					1064:1073	the simultaneous perception	1047:1073	the simultaneous perception of DAMPs and a PAMP	1047:1093	Here, we report that the simultaneous perception of DAMPs and a PAMP strengthens early and late plant defence responses.					
35697869	12	93	theme	downstream	1975:1984	arg1	responses					1986:1994	downstream responses	1975:1994	downstream responses	1975:1994	However, downstream responses seem more specific depending on the composition of the mixture.					
35697869	6	94	theme	classical	1171:1179	arg1	phosphorylation					1234:1248	MAPK phosphorylation	1229:1248	MAPK phosphorylation	1229:1248	To this aim, we studied classical PTI responses such as the generation of ROS and MAPK phosphorylation, but we also monitored the biosynthesis of phytocytokines and performed a non-targeted metabolomic analysis.					
35697869	6	94	theme	classical	1171:1179	arg1	generation					1207:1216	the generation	1203:1216	the generation of ROS	1203:1223	To this aim, we studied classical PTI responses such as the generation of ROS and MAPK phosphorylation, but we also monitored the biosynthesis of phytocytokines and performed a non-targeted metabolomic analysis.					
35697869	6	94	theme	classical	1171:1179	arg1	responses					1185:1193	classical PTI responses	1171:1193	classical PTI responses such as the generation of ROS and MAPK phosphorylation	1171:1248	To this aim, we studied classical PTI responses such as the generation of ROS and MAPK phosphorylation, but we also monitored the biosynthesis of phytocytokines and performed a non-targeted metabolomic analysis.					
35697869	3	95	theme	cell	572:575	arg1	fragments					582:590	cell wall fragments	572:590	cell wall fragments	572:590	In particular, DAMPs are intracellular molecules or cell wall fragments passive or actively released to the apoplast, whose extracellular recognition by intact cells triggers specific immune signalling, the so-called DAMP-triggered immunity.					
37294367	8	0	theme	NADH	1308:1311	arg1	detection					1295:1303	rapid detection	1289:1303	rapid detection of NADH in human serum	1289:1326	The SERS nanoprobe enabled rapid detection of NADH in human serum without any complicated sample pretreatment and provides a new potential to detect biomarkers.					
37294367	4	1	theme	S-CNF-Ag	609:616	arg1	substrate					621:629	The S-CNF-Ag NP substrate	605:629	The S-CNF-Ag NP substrate	605:629	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	0	2	theme	cellulose	52:60	arg1	nanofiber-Ag					62:73	sulfonated cellulose nanofiber-Ag	41:73	sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum	41:134	A novel "on-off" SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum.					
37294367	7	3	dep	established in	1208:1221	arg1	range					1241:1245	the concentration range 10-6 - 10-2 M	1223:1259	established in the concentration range 10-6 - 10-2 M	1208:1259	The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10-6 - 10-2 M.					
37294367	4	4	theme	1.23 × 107	755:764	arg1	uniformity					689:698	good uniformity	684:698	good uniformity with an RSD of 6.88%	684:719	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	4	4	theme	1.23 × 107	755:764	arg1	EF					748:749	EF	748:749	EF	748:749	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	4	4	theme	1.23 × 107	755:764	arg1	factor					740:745	an enhancement factor	725:745	an enhancement factor (EF) of 1.23 × 107	725:764	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	5	5	theme	charge	788:793	arg1	effect					805:810	the anionic charge repulsion effect	776:810	the anionic charge repulsion effect	776:810	Owing to the anionic charge repulsion effect, the S-CNF-Ag NP substrate still maintains remarkable dispersion stability after 12 months of preservation.					
37294367	1	6	theme	novel	139:143	arg1	nanocomposite					157:169	A novel S-CNF-based nanocomposite	137:169	A novel S-CNF-based nanocomposite	137:169	A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS).					
37294367	8	7	theme	rapid	1289:1293	arg1	detection					1295:1303	rapid detection	1289:1303	rapid detection of NADH in human serum	1289:1326	The SERS nanoprobe enabled rapid detection of NADH in human serum without any complicated sample pretreatment and provides a new potential to detect biomarkers.					
37294367	5	8	theme	remarkable	855:864	arg1	stability					877:885	remarkable dispersion stability	855:885	remarkable dispersion stability	855:885	Owing to the anionic charge repulsion effect, the S-CNF-Ag NP substrate still maintains remarkable dispersion stability after 12 months of preservation.					
37294367	6	9	mod	modified	955:962	arg3	molecule					1023:1030	a special redox Raman signal molecule	994:1030	a special redox Raman signal molecule	994:1030	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	6	9	mod	modified	955:962	arg3	4-MP					987:990	4-MP	987:990	4-MP	987:990	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	6	9	mod	modified	955:962	arg1	surface					943:949	S-CNF-Ag NPs' surface	929:949	S-CNF-Ag NPs' surface	929:949	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	6	9	mod	modified	955:962	arg3	4-mercaptophenol					969:984	4-mercaptophenol	969:984	4-mercaptophenol (4-MP)	969:991	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	0	10	theme	composite	75:83	arg1	nanofiber-Ag					62:73	sulfonated cellulose nanofiber-Ag	41:73	sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum	41:134	A novel "on-off" SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum.					
37294367	5	11	theme	dispersion	866:875	arg1	stability					877:885	remarkable dispersion stability	855:885	remarkable dispersion stability	855:885	Owing to the anionic charge repulsion effect, the S-CNF-Ag NP substrate still maintains remarkable dispersion stability after 12 months of preservation.					
37294367	4	12	theme	good	684:687	arg1	uniformity					689:698	good uniformity	684:698	good uniformity with an RSD of 6.88%	684:719	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	6	13	theme	redox	1004:1008	arg1	4-mercaptophenol					969:984	4-mercaptophenol	969:984	4-mercaptophenol (4-MP)	969:991	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	6	13	theme	redox	1004:1008	arg1	molecule					1023:1030	a special redox Raman signal molecule	994:1030	a special redox Raman signal molecule	994:1030	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	7	14	theme	good	1166:1169	arg1	established in					1208:1221	established in the concentration range 10-6 - 10-2 M	1208:1259	established in the concentration range 10-6 - 10-2 M	1208:1259	The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10-6 - 10-2 M.					
37294367	7	14	theme	good	1166:1169	arg1	relationship					1178:1189	a good linear relationship	1164:1189	a good linear relationship (R2 = 0.993)	1164:1202	The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10-6 - 10-2 M.					
37294367	7	14	theme	good	1166:1169	arg1	R2 = 0.993					1192:1201	R2 = 0.993	1192:1201	R2 = 0.993	1192:1201	The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10-6 - 10-2 M.					
37294367	6	15	theme	adenine	1064:1070	arg1	NADH					1086:1089	NADH	1086:1089	NADH	1086:1089	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	6	15	theme	adenine	1064:1070	arg1	dinucleotide					1072:1083	reduced nicotinamide adenine dinucleotide	1043:1083	reduced nicotinamide adenine dinucleotide (NADH)	1043:1090	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	1	16	theme	S-CNF-based	145:155	arg1	nanocomposite					157:169	A novel S-CNF-based nanocomposite	137:169	A novel S-CNF-based nanocomposite	137:169	A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS).					
37294367	0	17	theme	selective	89:97	arg1	determination					99:111	selective determination	89:111	selective determination of NADH in human serum	89:134	A novel "on-off" SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum.					
37294367	5	18	theme	repulsion	795:803	arg1	effect					805:810	the anionic charge repulsion effect	776:810	the anionic charge repulsion effect	776:810	Owing to the anionic charge repulsion effect, the S-CNF-Ag NP substrate still maintains remarkable dispersion stability after 12 months of preservation.					
37294367	2	19	from	hydroxyl	329:336	arg1	surface					376:382	the S-CNF surface	366:382	the S-CNF surface	366:382	The numerous hydroxyl and sulfonic acid groups on the S-CNF surface absorbed silver ions and converted them to silver seeds, which formed the load fulcrum.					
37294367	3	20	theme	reducing	487:494	arg1	agent					496:500	a reducing agent	485:500	a reducing agent	485:500	After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D "hot spots."					
37294367	0	21	theme	novel	2:6	arg1	"					15:15	A novel "on-off"	0:15	A novel "on-off" SERS	0:20	A novel "on-off" SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum.					
37294367	6	22	theme	reduced	1043:1049	arg1	NADH					1086:1089	NADH	1086:1089	NADH	1086:1089	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	6	22	theme	reduced	1043:1049	arg1	dinucleotide					1072:1083	reduced nicotinamide adenine dinucleotide	1043:1083	reduced nicotinamide adenine dinucleotide (NADH)	1043:1090	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	2	23	theme	load	458:461	arg1	fulcrum					463:469	the load fulcrum	454:469	the load fulcrum	454:469	The numerous hydroxyl and sulfonic acid groups on the S-CNF surface absorbed silver ions and converted them to silver seeds, which formed the load fulcrum.					
37294367	5	24	theme	NP	826:827	arg1	substrate					829:837	the S-CNF-Ag NP substrate	813:837	the S-CNF-Ag NP substrate	813:837	Owing to the anionic charge repulsion effect, the S-CNF-Ag NP substrate still maintains remarkable dispersion stability after 12 months of preservation.					
37294367	7	25	theme	linear	1171:1176	arg1	established in					1208:1221	established in the concentration range 10-6 - 10-2 M	1208:1259	established in the concentration range 10-6 - 10-2 M	1208:1259	The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10-6 - 10-2 M.					
37294367	7	25	theme	linear	1171:1176	arg1	relationship					1178:1189	a good linear relationship	1164:1189	a good linear relationship (R2 = 0.993)	1164:1202	The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10-6 - 10-2 M.					
37294367	7	25	theme	linear	1171:1176	arg1	R2 = 0.993					1192:1201	R2 = 0.993	1192:1201	R2 = 0.993	1192:1201	The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10-6 - 10-2 M.					
37294367	8	26	theme	new	1387:1389	arg1	potential					1391:1399	a new potential	1385:1399	a new potential to detect biomarkers	1385:1420	The SERS nanoprobe enabled rapid detection of NADH in human serum without any complicated sample pretreatment and provides a new potential to detect biomarkers.					
37294367	1	27	theme	NADH	255:258	arg1	detection					242:250	the detection	238:250	the detection of NADH in serum	238:267	A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS).					
37294367	3	28	theme	Ag	525:526	arg1	nanoparticles					510:522	silver nanoparticles	503:522	silver nanoparticles (Ag NPs)	503:531	After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D "hot spots."					
37294367	3	28	theme	Ag	525:526	arg1	NPs					528:530	Ag NPs	525:530	Ag NPs	525:530	After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D "hot spots."					
37294367	2	29	theme	acid	351:354	arg1	groups					356:361	The numerous hydroxyl and sulfonic acid groups	316:361	groups	356:361	The numerous hydroxyl and sulfonic acid groups on the S-CNF surface absorbed silver ions and converted them to silver seeds, which formed the load fulcrum.					
37294367	0	30	theme	on-off	9:14	arg1	"					15:15	A novel "on-off"	0:15	A novel "on-off" SERS	0:20	A novel "on-off" SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum.					
37294367	5	31	theme	preservation	906:917	arg1	12 months					893:901	12 months	893:901	12 months of preservation	893:917	Owing to the anionic charge repulsion effect, the S-CNF-Ag NP substrate still maintains remarkable dispersion stability after 12 months of preservation.					
37294367	0	32	theme	NADH	116:119	arg1	determination					99:111	selective determination	89:111	selective determination of NADH in human serum	89:134	A novel "on-off" SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum.					
37294367	3	33	theme	silver	503:508	arg1	nanoparticles					510:522	silver nanoparticles	503:522	silver nanoparticles (Ag NPs)	503:531	After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D "hot spots."					
37294367	3	33	theme	silver	503:508	arg1	NPs					528:530	Ag NPs	525:530	Ag NPs	525:530	After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D "hot spots."					
37294367	2	34	theme	silver	393:398	arg1	ions					400:403	silver ions	393:403	silver ions	393:403	The numerous hydroxyl and sulfonic acid groups on the S-CNF surface absorbed silver ions and converted them to silver seeds, which formed the load fulcrum.					
37294367	4	35	theme	SERS	656:659	arg1	performance					661:671	outstanding SERS performance	644:671	outstanding SERS performance	644:671	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	4	36	with	uniformity	689:698	arg1	RSD					708:710	an RSD	705:710	an RSD of 6.88%	705:719	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	8	37	theme	human	1316:1320	arg1	serum					1322:1326	human serum	1316:1326	human serum	1316:1326	The SERS nanoprobe enabled rapid detection of NADH in human serum without any complicated sample pretreatment and provides a new potential to detect biomarkers.					
37294367	2	38	theme	numerous	320:327	arg1	hydroxyl					329:336	The numerous hydroxyl and sulfonic acid groups	316:361	hydroxyl	329:336	The numerous hydroxyl and sulfonic acid groups on the S-CNF surface absorbed silver ions and converted them to silver seeds, which formed the load fulcrum.					
37294367	8	39	from	detection	1295:1303	arg1	serum					1322:1326	human serum	1316:1326	human serum	1316:1326	The SERS nanoprobe enabled rapid detection of NADH in human serum without any complicated sample pretreatment and provides a new potential to detect biomarkers.					
37294367	1	40	theme	sulfonated	189:198	arg1	S-CNF					221:225	S-CNF	221:225	S-CNF	221:225	A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS).					
37294367	1	40	theme	sulfonated	189:198	arg1	nanofiber					210:218	sulfonated cellulose nanofiber	189:218	sulfonated cellulose nanofiber (S-CNF)	189:226	A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS).					
37294367	6	41	theme	signal	1016:1021	arg1	4-mercaptophenol					969:984	4-mercaptophenol	969:984	4-mercaptophenol (4-MP)	969:991	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	6	41	theme	signal	1016:1021	arg1	molecule					1023:1030	a special redox Raman signal molecule	994:1030	a special redox Raman signal molecule	994:1030	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	0	42	theme	human	124:128	arg1	serum					130:134	human serum	124:134	human serum	124:134	A novel "on-off" SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum.					
37294367	3	43	theme	stable	582:587	arg1	spots					597:601	stable 1D "hot spots	582:601	stable 1D "hot spots	582:601	After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D "hot spots."					
37294367	8	44	theme	SERS	1266:1269	arg1	nanoprobe					1271:1279	The SERS nanoprobe	1262:1279	The SERS nanoprobe	1262:1279	The SERS nanoprobe enabled rapid detection of NADH in human serum without any complicated sample pretreatment and provides a new potential to detect biomarkers.					
37294367	1	45	theme	cellulose	200:208	arg1	S-CNF					221:225	S-CNF	221:225	S-CNF	221:225	A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS).					
37294367	1	45	theme	cellulose	200:208	arg1	nanofiber					210:218	sulfonated cellulose nanofiber	189:218	sulfonated cellulose nanofiber (S-CNF)	189:226	A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS).					
37294367	0	46	theme	"	15:15	arg1	SERS					17:20	A novel "on-off" SERS	0:20	A novel "on-off" SERS	0:20	A novel "on-off" SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum.					
37294367	1	47	theme	surface-enhanced	272:287	arg1	SERS					309:312	SERS	309:312	SERS	309:312	A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS).					
37294367	1	47	theme	surface-enhanced	272:287	arg1	spectroscopy					295:306	surface-enhanced Raman spectroscopy	272:306	surface-enhanced Raman spectroscopy (SERS)	272:313	A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS).					
37294367	0	48	dep	nanoprobe	22:30	arg1	based					32:36	based	32:36	nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum	22:134	A novel "on-off" SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum.					
37294367	2	49	from	groups	356:361	arg1	surface					376:382	the S-CNF surface	366:382	the S-CNF surface	366:382	The numerous hydroxyl and sulfonic acid groups on the S-CNF surface absorbed silver ions and converted them to silver seeds, which formed the load fulcrum.					
37294367	2	50	theme	S-CNF	370:374	arg1	surface					376:382	the S-CNF surface	366:382	the S-CNF surface	366:382	The numerous hydroxyl and sulfonic acid groups on the S-CNF surface absorbed silver ions and converted them to silver seeds, which formed the load fulcrum.					
37294367	2	51	theme	silver	427:432	arg1	seeds					434:438	silver seeds	427:438	silver seeds	427:438	The numerous hydroxyl and sulfonic acid groups on the S-CNF surface absorbed silver ions and converted them to silver seeds, which formed the load fulcrum.					
37294367	1	52	theme	Raman	289:293	arg1	SERS					309:312	SERS	309:312	SERS	309:312	A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS).					
37294367	1	52	theme	Raman	289:293	arg1	spectroscopy					295:306	surface-enhanced Raman spectroscopy	272:306	surface-enhanced Raman spectroscopy (SERS)	272:313	A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS).					
37294367	8	53	theme	complicated	1340:1350	arg1	pretreatment					1359:1370	any complicated sample pretreatment	1336:1370	any complicated sample pretreatment	1336:1370	The SERS nanoprobe enabled rapid detection of NADH in human serum without any complicated sample pretreatment and provides a new potential to detect biomarkers.					
37294367	7	54	theme	concentration	1227:1239	arg1	range					1241:1245	the concentration range 10-6 - 10-2 M	1223:1259	established in the concentration range 10-6 - 10-2 M	1208:1259	The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10-6 - 10-2 M.					
37294367	6	55	theme	special	996:1002	arg1	4-mercaptophenol					969:984	4-mercaptophenol	969:984	4-mercaptophenol (4-MP)	969:991	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	6	55	theme	special	996:1002	arg1	molecule					1023:1030	a special redox Raman signal molecule	994:1030	a special redox Raman signal molecule	994:1030	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	7	56	theme	NADH	1146:1149	arg1	LOD					1138:1140	LOD	1138:1140	LOD	1138:1140	The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10-6 - 10-2 M.					
37294367	7	56	theme	NADH	1146:1149	arg1	limit					1131:1135	the detection limit	1117:1135	the detection limit (LOD) of NADH	1117:1149	The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10-6 - 10-2 M.					
37294367	1	57	from	detection	242:250	arg1	serum					263:267	serum	263:267	serum	263:267	A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS).					
37294367	0	58	from	determination	99:111	arg1	serum					130:134	human serum	124:134	human serum	124:134	A novel "on-off" SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum.					
37294367	4	59	theme	enhancement	728:738	arg1	EF					748:749	EF	748:749	EF	748:749	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	4	59	theme	enhancement	728:738	arg1	factor					740:745	an enhancement factor	725:745	an enhancement factor (EF) of 1.23 × 107	725:764	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	4	60	theme	NP	618:619	arg1	substrate					621:629	The S-CNF-Ag NP substrate	605:629	The S-CNF-Ag NP substrate	605:629	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	6	61	theme	S-CNF-Ag	929:936	arg1	surface					943:949	S-CNF-Ag NPs' surface	929:949	S-CNF-Ag NPs' surface	929:949	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	4	62	theme	outstanding	644:654	arg1	performance					661:671	outstanding SERS performance	644:671	outstanding SERS performance	644:671	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	4	63	with	factor	740:745	arg1	RSD					708:710	an RSD	705:710	an RSD of 6.88%	705:719	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	6	64	theme	nicotinamide	1051:1062	arg1	NADH					1086:1089	NADH	1086:1089	NADH	1086:1089	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	6	64	theme	nicotinamide	1051:1062	arg1	dinucleotide					1072:1083	reduced nicotinamide adenine dinucleotide	1043:1083	reduced nicotinamide adenine dinucleotide (NADH)	1043:1090	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	3	65	theme	1D	589:590	arg1	spots					597:601	stable 1D "hot spots	582:601	stable 1D "hot spots	582:601	After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D "hot spots."					
37294367	3	66	theme	S-CNF	560:564	arg1	surface					566:572	the S-CNF surface	556:572	the S-CNF surface	556:572	After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D "hot spots."					
37294367	5	67	theme	S-CNF-Ag	817:824	arg1	substrate					829:837	the S-CNF-Ag NP substrate	813:837	the S-CNF-Ag NP substrate	813:837	Owing to the anionic charge repulsion effect, the S-CNF-Ag NP substrate still maintains remarkable dispersion stability after 12 months of preservation.					
37294367	6	68	theme	Raman	1010:1014	arg1	4-mercaptophenol					969:984	4-mercaptophenol	969:984	4-mercaptophenol (4-MP)	969:991	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	6	68	theme	Raman	1010:1014	arg1	molecule					1023:1030	a special redox Raman signal molecule	994:1030	a special redox Raman signal molecule	994:1030	Finally, S-CNF-Ag NPs' surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH).					
37294367	0	69	theme	sulfonated	41:50	arg1	nanofiber-Ag					62:73	sulfonated cellulose nanofiber-Ag	41:73	sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum	41:134	A novel "on-off" SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum.					
37294367	8	70	theme	sample	1352:1357	arg1	pretreatment					1359:1370	any complicated sample pretreatment	1336:1370	any complicated sample pretreatment	1336:1370	The SERS nanoprobe enabled rapid detection of NADH in human serum without any complicated sample pretreatment and provides a new potential to detect biomarkers.					
37294367	4	71	theme	%	719:719	arg1	RSD					708:710	an RSD	705:710	an RSD of 6.88%	705:719	The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107.					
37294367	3	72	dep	adhered	545:551	arg1	"					603:603	"	603:603	"	603:603	After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D "hot spots."					
37294367	2	73	theme	sulfonic	342:349	arg1	groups					356:361	The numerous hydroxyl and sulfonic acid groups	316:361	groups	356:361	The numerous hydroxyl and sulfonic acid groups on the S-CNF surface absorbed silver ions and converted them to silver seeds, which formed the load fulcrum.					
37294367	3	74	theme	hot	593:595	arg1	spots					597:601	stable 1D "hot spots	582:601	stable 1D "hot spots	582:601	After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D "hot spots."					
37294367	5	75	theme	anionic	780:786	arg1	effect					805:810	the anionic charge repulsion effect	776:810	the anionic charge repulsion effect	776:810	Owing to the anionic charge repulsion effect, the S-CNF-Ag NP substrate still maintains remarkable dispersion stability after 12 months of preservation.					
37294367	7	76	theme	detection	1121:1129	arg1	LOD					1138:1140	LOD	1138:1140	LOD	1138:1140	The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10-6 - 10-2 M.					
37294367	7	76	theme	detection	1121:1129	arg1	limit					1131:1135	the detection limit	1117:1135	the detection limit (LOD) of NADH	1117:1149	The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10-6 - 10-2 M.					
35993784	6	0	theme	degradation	952:962	arg1	efficiency					964:973	higher degradation efficiency	945:973	higher degradation efficiency	945:973	AZA_4_5 and the natural system showed higher degradation efficiency than other single strains or the artificial system.					
35993784	2	1	theme	beetle	368:373	arg1	microbiomes					342:352	the gut microbiomes	334:352	the gut microbiomes of rhinoceros beetle (Trypoxylus dichotomus) feeding on bamboo and wood fiber	334:430	This study compared the gut microbiomes of rhinoceros beetle (Trypoxylus dichotomus) feeding on bamboo and wood fiber.					
35993784	0	2	theme	Lignocellulose	75:88	arg1	Degradation					90:100	Bamboo Lignocellulose Degradation	68:100	Bamboo Lignocellulose Degradation	68:100	Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation.					
35993784	6	3	theme	higher	945:950	arg1	efficiency					964:973	higher degradation efficiency	945:973	higher degradation efficiency	945:973	AZA_4_5 and the natural system showed higher degradation efficiency than other single strains or the artificial system.					
35993784	3	4	from	composition	502:512	arg1	midgut					521:526	the midgut	517:526	the midgut	517:526	The results revealed that diet has a distinctive effect on microbial composition in the midgut, including its most abundant microorganisms that in the fermentation and chemoheterotroph pathways.					
35993784	2	5	theme	rhinoceros	357:366	arg1	beetle					368:373	rhinoceros beetle	357:373	rhinoceros beetle (Trypoxylus dichotomus)	357:397	This study compared the gut microbiomes of rhinoceros beetle (Trypoxylus dichotomus) feeding on bamboo and wood fiber.					
35993784	12	6	theme	lignocellulosic	1770:1784	arg1	biomass					1786:1792	the bamboo lignocellulosic biomass	1759:1792	the bamboo lignocellulosic biomass	1759:1792	The results indicate that the gut bacteria could provide an effective system to utilize the bamboo lignocellulosic biomass.					
35993784	7	7	theme	consortium	1139:1148	arg1	development					1102:1112	development	1102:1112	development of a synthetic bacterial consortium	1102:1148	The results can thus serve as important reference for further research and development of a synthetic bacterial consortium to maximize lignocellulolytic ability.					
35993784	7	7	theme	consortium	1139:1148	arg1	research					1089:1096	further research	1081:1096	further research	1081:1096	The results can thus serve as important reference for further research and development of a synthetic bacterial consortium to maximize lignocellulolytic ability.					
35993784	2	8	dep	beetle	368:373	arg1	dichotomus					387:396	Trypoxylus dichotomus	376:396	Trypoxylus dichotomus	376:396	This study compared the gut microbiomes of rhinoceros beetle (Trypoxylus dichotomus) feeding on bamboo and wood fiber.					
35993784	12	9	theme	bamboo	1763:1768	arg1	biomass					1786:1792	the bamboo lignocellulosic biomass	1759:1792	the bamboo lignocellulosic biomass	1759:1792	The results indicate that the gut bacteria could provide an effective system to utilize the bamboo lignocellulosic biomass.					
35993784	1	10	theme	bamboo	265:270	arg1	decomposition					282:294	bamboo cellulose decomposition	265:294	bamboo cellulose decomposition	265:294	Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed.					
35993784	6	11	theme	artificial	1008:1017	arg1	system					1019:1024	the artificial system	1004:1024	the artificial system	1004:1024	AZA_4_5 and the natural system showed higher degradation efficiency than other single strains or the artificial system.					
35993784	9	12	theme	wood	1374:1377	arg1	diets					1385:1389	wood fiber diets	1374:1389	wood fiber diets	1374:1389	The gut microbiome of Trypoxylus dichotomus differed between bamboo and wood fiber diets.					
35993784	4	13	theme	artificial	774:783	arg1	system					795:800	an artificial bacterial system	771:800	an artificial bacterial system	771:800	After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose.					
35993784	4	14	theme	microbial	738:746	arg1	abundance					757:765	the microbial relative abundance	734:765	the microbial relative abundance	734:765	After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose.					
35993784	12	15	theme	effective	1731:1739	arg1	system					1741:1746	an effective system	1728:1746	an effective system to utilize the bamboo lignocellulosic biomass	1728:1792	The results indicate that the gut bacteria could provide an effective system to utilize the bamboo lignocellulosic biomass.					
35993784	1	16	theme	cellulose	272:280	arg1	decomposition					282:294	bamboo cellulose decomposition	265:294	bamboo cellulose decomposition	265:294	Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed.					
35993784	8	17	theme	lignocellulosic	1233:1247	arg1	biomass					1249:1255	lignocellulosic biomass	1233:1255	lignocellulosic biomass due to its high efficiency in carbon fixing	1233:1299	IMPORTANCE Bamboo produces a great yield of lignocellulosic biomass due to its high efficiency in carbon fixing.					
35993784	6	18	theme	natural	923:929	arg1	system					931:936	the natural system	919:936	the natural system	919:936	AZA_4_5 and the natural system showed higher degradation efficiency than other single strains or the artificial system.					
35993784	1	19	with	source	132:137	arg1	potential					174:182	the potential to contribute to energy sustainability	170:221	the potential to contribute to energy sustainability	170:221	Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed.					
35993784	2	20	from	microbiomes	342:352	arg1	bamboo					410:415	bamboo	410:415	bamboo	410:415	This study compared the gut microbiomes of rhinoceros beetle (Trypoxylus dichotomus) feeding on bamboo and wood fiber.					
35993784	2	20	from	microbiomes	342:352	arg1	fiber					426:430	wood fiber	421:430	wood fiber	421:430	This study compared the gut microbiomes of rhinoceros beetle (Trypoxylus dichotomus) feeding on bamboo and wood fiber.					
35993784	5	21	theme	Enterobacter	890:901	arg1	sp					903:904	The isolate Enterobacter sp	878:904	The isolate Enterobacter sp.	878:905	The isolate Enterobacter sp.					
35993784	8	22	from	efficiency	1273:1282	arg1	fixing					1294:1299	carbon fixing	1287:1299	carbon fixing	1287:1299	IMPORTANCE Bamboo produces a great yield of lignocellulosic biomass due to its high efficiency in carbon fixing.					
35993784	8	23	theme	biomass	1249:1255	arg1	yield					1224:1228	a great yield	1216:1228	a great yield of lignocellulosic biomass due to its high efficiency in carbon fixing	1216:1299	IMPORTANCE Bamboo produces a great yield of lignocellulosic biomass due to its high efficiency in carbon fixing.					
35993784	11	24	theme	efficient	1486:1494	arg1	isolates					1506:1513	The highly efficient bacterial isolates	1475:1513	The highly efficient bacterial isolates	1475:1513	The highly efficient bacterial isolates were identified from midgut, whereas the natural bacterial system as well as one isolate showed the higher degradation efficiency of bamboo lignocellulose.					
35993784	8	25	theme	due	1257:1259	arg1	biomass					1249:1255	lignocellulosic biomass	1233:1255	lignocellulosic biomass due to its high efficiency in carbon fixing	1233:1299	IMPORTANCE Bamboo produces a great yield of lignocellulosic biomass due to its high efficiency in carbon fixing.					
35993784	9	26	theme	dichotomus	1335:1344	arg1	microbiome					1310:1319	The gut microbiome	1302:1319	The gut microbiome of Trypoxylus dichotomus	1302:1344	The gut microbiome of Trypoxylus dichotomus differed between bamboo and wood fiber diets.					
35993784	7	27	theme	synthetic	1119:1127	arg1	consortium					1139:1148	a synthetic bacterial consortium	1117:1148	a synthetic bacterial consortium	1117:1148	The results can thus serve as important reference for further research and development of a synthetic bacterial consortium to maximize lignocellulolytic ability.					
35993784	9	28	theme	fiber	1379:1383	arg1	diets					1385:1389	wood fiber diets	1374:1389	wood fiber diets	1374:1389	The gut microbiome of Trypoxylus dichotomus differed between bamboo and wood fiber diets.					
35993784	0	29	theme	Trypoxylus	0:9	arg1	Bacteria					26:33	Trypoxylus dichotomus Gut Bacteria	0:33	Trypoxylus dichotomus Gut Bacteria	0:33	Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation.					
35993784	7	30	theme	bacterial	1129:1137	arg1	consortium					1139:1148	a synthetic bacterial consortium	1117:1148	a synthetic bacterial consortium	1117:1148	The results can thus serve as important reference for further research and development of a synthetic bacterial consortium to maximize lignocellulolytic ability.					
35993784	11	31	theme	higher	1615:1620	arg1	efficiency					1634:1643	the higher degradation efficiency	1611:1643	the higher degradation efficiency of bamboo lignocellulose	1611:1668	The highly efficient bacterial isolates were identified from midgut, whereas the natural bacterial system as well as one isolate showed the higher degradation efficiency of bamboo lignocellulose.					
35993784	1	32	theme	Fast-growing	103:114	arg1	source					132:137	a source	130:137	a source of high-quality cellulose with the potential to contribute to energy sustainability	130:221	Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed.					
35993784	1	32	theme	Fast-growing	103:114	arg1	bamboo					116:121	Fast-growing bamboo	103:121	Fast-growing bamboo	103:121	Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed.					
35993784	4	33	theme	bacterial	785:793	arg1	system					795:800	an artificial bacterial system	771:800	an artificial bacterial system	771:800	After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose.					
35993784	4	34	theme	relative	748:755	arg1	abundance					757:765	the microbial relative abundance	734:765	the microbial relative abundance	734:765	After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose.					
35993784	7	35	theme	lignocellulolytic	1162:1178	arg1	ability					1180:1186	lignocellulolytic ability	1162:1186	lignocellulolytic ability	1162:1186	The results can thus serve as important reference for further research and development of a synthetic bacterial consortium to maximize lignocellulolytic ability.					
35993784	10	36	theme	diet	1469:1472	arg1	bacteria					1446:1453	the gut bacteria	1438:1453	the gut bacteria of the bamboo diet	1438:1472	The lignocellulosic pathways were enriched in the gut bacteria of the bamboo diet.					
35993784	3	37	theme	abundant	548:555	arg1	microorganisms					557:570	its most abundant microorganisms	539:570	its most abundant microorganisms that in the fermentation and chemoheterotroph pathways	539:625	The results revealed that diet has a distinctive effect on microbial composition in the midgut, including its most abundant microorganisms that in the fermentation and chemoheterotroph pathways.					
35993784	10	38	theme	bamboo	1462:1467	arg1	diet					1469:1472	the bamboo diet	1458:1472	the bamboo diet	1458:1472	The lignocellulosic pathways were enriched in the gut bacteria of the bamboo diet.					
35993784	1	39	theme	energy	201:206	arg1	sustainability					208:221	energy sustainability	201:221	energy sustainability	201:221	Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed.					
35993784	0	40	theme	Gut	22:24	arg1	Bacteria					26:33	Trypoxylus dichotomus Gut Bacteria	0:33	Trypoxylus dichotomus Gut Bacteria	0:33	Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation.					
35993784	11	41	theme	bamboo	1648:1653	arg1	lignocellulose					1655:1668	bamboo lignocellulose	1648:1668	bamboo lignocellulose	1648:1668	The highly efficient bacterial isolates were identified from midgut, whereas the natural bacterial system as well as one isolate showed the higher degradation efficiency of bamboo lignocellulose.					
35993784	9	42	theme	gut	1306:1308	arg1	microbiome					1310:1319	The gut microbiome	1302:1319	The gut microbiome of Trypoxylus dichotomus	1302:1344	The gut microbiome of Trypoxylus dichotomus differed between bamboo and wood fiber diets.					
35993784	4	43	theme	bacterial	708:716	arg1	system					718:723	a natural bacterial system	698:723	a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate	698:839	After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose.					
35993784	2	44	theme	wood	421:424	arg1	fiber					426:430	wood fiber	421:430	wood fiber	421:430	This study compared the gut microbiomes of rhinoceros beetle (Trypoxylus dichotomus) feeding on bamboo and wood fiber.					
35993784	4	45	theme	bacterial	663:671	arg1	isolates					673:680	the 13 efficient bacterial isolates	646:680	the 13 efficient bacterial isolates	646:680	After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose.					
35993784	4	46	theme	natural	700:706	arg1	system					718:723	a natural bacterial system	698:723	a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate	698:839	After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose.					
35993784	8	47	theme	high	1268:1271	arg1	efficiency					1273:1282	its high efficiency	1264:1282	its high efficiency in carbon fixing	1264:1299	IMPORTANCE Bamboo produces a great yield of lignocellulosic biomass due to its high efficiency in carbon fixing.					
35993784	3	48	theme	chemoheterotroph	601:616	arg1	pathways					618:625	the fermentation and chemoheterotroph pathways	580:625	pathways	618:625	The results revealed that diet has a distinctive effect on microbial composition in the midgut, including its most abundant microorganisms that in the fermentation and chemoheterotroph pathways.					
35993784	4	49	theme	efficient	653:661	arg1	isolates					673:680	the 13 efficient bacterial isolates	646:680	the 13 efficient bacterial isolates	646:680	After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose.					
35993784	8	50	theme	carbon	1287:1292	arg1	fixing					1294:1299	carbon fixing	1287:1299	carbon fixing	1287:1299	IMPORTANCE Bamboo produces a great yield of lignocellulosic biomass due to its high efficiency in carbon fixing.					
35993784	6	51	theme	single	986:991	arg1	strains					993:999	other single strains	980:999	other single strains	980:999	AZA_4_5 and the natural system showed higher degradation efficiency than other single strains or the artificial system.					
35993784	7	52	theme	further	1081:1087	arg1	research					1089:1096	further research	1081:1096	further research	1081:1096	The results can thus serve as important reference for further research and development of a synthetic bacterial consortium to maximize lignocellulolytic ability.					
35993784	3	53	contain	has	464:466	arg1	diet					459:462	diet	459:462	diet	459:462	The results revealed that diet has a distinctive effect on microbial composition in the midgut, including its most abundant microorganisms that in the fermentation and chemoheterotroph pathways.					
35993784	3	53	contain	has	464:466	arg2	effect					482:487	a distinctive effect	468:487	a distinctive effect	468:487	The results revealed that diet has a distinctive effect on microbial composition in the midgut, including its most abundant microorganisms that in the fermentation and chemoheterotroph pathways.					
35993784	4	54	theme	bamboo	855:860	arg1	lignocellulose					862:875	bamboo lignocellulose	855:875	bamboo lignocellulose	855:875	After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose.					
35993784	7	55	theme	important	1057:1065	arg1	reference					1067:1075	important reference	1057:1075	important reference for further research and development of a synthetic bacterial consortium	1057:1148	The results can thus serve as important reference for further research and development of a synthetic bacterial consortium to maximize lignocellulolytic ability.					
35993784	0	56	theme	Effective	47:55	arg1	System					57:62	an Effective System	44:62	an Effective System for Bamboo Lignocellulose Degradation	44:100	Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation.					
35993784	6	57	theme	other	980:984	arg1	strains					993:999	other single strains	980:999	other single strains	980:999	AZA_4_5 and the natural system showed higher degradation efficiency than other single strains or the artificial system.					
35993784	3	58	theme	distinctive	470:480	arg1	effect					482:487	a distinctive effect	468:487	a distinctive effect	468:487	The results revealed that diet has a distinctive effect on microbial composition in the midgut, including its most abundant microorganisms that in the fermentation and chemoheterotroph pathways.					
35993784	3	59	theme	fermentation	584:595	arg1	pathways					618:625	the fermentation and chemoheterotroph pathways	580:625	pathways	618:625	The results revealed that diet has a distinctive effect on microbial composition in the midgut, including its most abundant microorganisms that in the fermentation and chemoheterotroph pathways.					
35993784	10	60	theme	lignocellulosic	1396:1410	arg1	pathways					1412:1419	The lignocellulosic pathways	1392:1419	The lignocellulosic pathways	1392:1419	The lignocellulosic pathways were enriched in the gut bacteria of the bamboo diet.					
35993784	3	61	theme	microbial	492:500	arg1	composition					502:512	microbial composition	492:512	microbial composition	492:512	The results revealed that diet has a distinctive effect on microbial composition in the midgut, including its most abundant microorganisms that in the fermentation and chemoheterotroph pathways.					
35993784	11	62	theme	natural	1556:1562	arg1	system					1574:1579	the natural bacterial system	1552:1579	the natural bacterial system as well as one isolate	1552:1602	The highly efficient bacterial isolates were identified from midgut, whereas the natural bacterial system as well as one isolate showed the higher degradation efficiency of bamboo lignocellulose.					
35993784	11	63	theme	degradation	1622:1632	arg1	efficiency					1634:1643	the higher degradation efficiency	1611:1643	the higher degradation efficiency of bamboo lignocellulose	1611:1668	The highly efficient bacterial isolates were identified from midgut, whereas the natural bacterial system as well as one isolate showed the higher degradation efficiency of bamboo lignocellulose.					
35993784	8	64	theme	great	1218:1222	arg1	yield					1224:1228	a great yield	1216:1228	a great yield of lignocellulosic biomass due to its high efficiency in carbon fixing	1216:1299	IMPORTANCE Bamboo produces a great yield of lignocellulosic biomass due to its high efficiency in carbon fixing.					
35993784	12	65	theme	gut	1701:1703	arg1	bacteria					1705:1712	the gut bacteria	1697:1712	the gut bacteria	1697:1712	The results indicate that the gut bacteria could provide an effective system to utilize the bamboo lignocellulosic biomass.					
35993784	4	66	theme	isolate	833:839	arg1	proportions					813:823	equal proportions	807:823	equal proportions of each isolate	807:839	After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose.					
35993784	2	67	theme	gut	338:340	arg1	microbiomes					342:352	the gut microbiomes	334:352	the gut microbiomes of rhinoceros beetle (Trypoxylus dichotomus) feeding on bamboo and wood fiber	334:430	This study compared the gut microbiomes of rhinoceros beetle (Trypoxylus dichotomus) feeding on bamboo and wood fiber.					
35993784	3	68	from	microorganisms	557:570	arg1	pathways					618:625	the fermentation and chemoheterotroph pathways	580:625	pathways	618:625	The results revealed that diet has a distinctive effect on microbial composition in the midgut, including its most abundant microorganisms that in the fermentation and chemoheterotroph pathways.					
35993784	11	69	theme	lignocellulose	1655:1668	arg1	efficiency					1634:1643	the higher degradation efficiency	1611:1643	the higher degradation efficiency of bamboo lignocellulose	1611:1668	The highly efficient bacterial isolates were identified from midgut, whereas the natural bacterial system as well as one isolate showed the higher degradation efficiency of bamboo lignocellulose.					
35993784	11	70	theme	bacterial	1496:1504	arg1	isolates					1506:1513	The highly efficient bacterial isolates	1475:1513	The highly efficient bacterial isolates	1475:1513	The highly efficient bacterial isolates were identified from midgut, whereas the natural bacterial system as well as one isolate showed the higher degradation efficiency of bamboo lignocellulose.					
35993784	4	71	theme	equal	807:811	arg1	proportions					813:823	equal proportions	807:823	equal proportions of each isolate	807:839	After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose.					
35993784	10	72	theme	gut	1442:1444	arg1	bacteria					1446:1453	the gut bacteria	1438:1453	the gut bacteria of the bamboo diet	1438:1472	The lignocellulosic pathways were enriched in the gut bacteria of the bamboo diet.					
35993784	3	73	dep	microorganisms	557:570	arg1	that					572:575	that	572:575	that	572:575	The results revealed that diet has a distinctive effect on microbial composition in the midgut, including its most abundant microorganisms that in the fermentation and chemoheterotroph pathways.					
35993784	1	74	theme	efficient	230:238	arg1	solution					253:260	an efficient and low-cost solution	227:260	an efficient and low-cost solution to bamboo cellulose decomposition	227:294	Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed.					
35993784	0	75	theme	Bamboo	68:73	arg1	Degradation					90:100	Bamboo Lignocellulose Degradation	68:100	Bamboo Lignocellulose Degradation	68:100	Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation.					
35993784	5	76	theme	isolate	882:888	arg1	sp					903:904	The isolate Enterobacter sp	878:904	The isolate Enterobacter sp.	878:905	The isolate Enterobacter sp.					
35993784	1	77	theme	high-quality	142:153	arg1	cellulose					155:163	high-quality cellulose	142:163	high-quality cellulose	142:163	Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed.					
35993784	11	78	theme	bacterial	1564:1572	arg1	system					1574:1579	the natural bacterial system	1552:1579	the natural bacterial system as well as one isolate	1552:1602	The highly efficient bacterial isolates were identified from midgut, whereas the natural bacterial system as well as one isolate showed the higher degradation efficiency of bamboo lignocellulose.					
35993784	0	79	dep	Trypoxylus	0:9	arg1	dichotomus					11:20	dichotomus	11:20	dichotomus	11:20	Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation.					
35993784	1	80	theme	cellulose	155:163	arg1	source					132:137	a source	130:137	a source of high-quality cellulose with the potential to contribute to energy sustainability	130:221	Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed.					
35993784	1	80	theme	cellulose	155:163	arg1	bamboo					116:121	Fast-growing bamboo	103:121	Fast-growing bamboo	103:121	Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed.					
35993784	1	81	theme	low-cost	244:251	arg1	solution					253:260	an efficient and low-cost solution	227:260	an efficient and low-cost solution to bamboo cellulose decomposition	227:294	Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed.					
35926287	2	0	theme	AgNCs	423:427	arg1	loading					412:418	loading	412:418	loading of AgNCs	412:427	The surface of MSNs was coated by CS as the gatekeeper and the template for loading of AgNCs.					
35926287	6	1	theme	Ag	890:891	arg1	nanoparticles					893:905	The MSN/TPE-CS@Ag nanoparticles	875:905	The MSN/TPE-CS@Ag nanoparticles	875:905	The MSN/TPE-CS@Ag nanoparticles showed good sensitivity and selectivity for H2O2 even with various interfering ions and agents.					
35926287	1	2	theme	silver	306:311	arg1	AgNCs					328:332	AgNCs	328:332	AgNCs	328:332	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	1	2	theme	silver	306:311	arg1	nanoparticles					313:325	1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles	252:325	1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs)	252:333	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	8	3	theme	practical	1206:1214	arg1	samples					1216:1222	practical samples	1206:1222	practical samples	1206:1222	The feasibility of the practical application of this probe was confirmed by accurate quantitative of H2O2 in practical samples.					
35926287	6	4	theme	@	889:889	arg1	nanoparticles					893:905	The MSN/TPE-CS@Ag nanoparticles	875:905	The MSN/TPE-CS@Ag nanoparticles	875:905	The MSN/TPE-CS@Ag nanoparticles showed good sensitivity and selectivity for H2O2 even with various interfering ions and agents.					
35926287	8	5	from	quantitative	1182:1193	arg1	samples					1216:1222	practical samples	1206:1222	practical samples	1206:1222	The feasibility of the practical application of this probe was confirmed by accurate quantitative of H2O2 in practical samples.					
35926287	6	6	theme	MSN/TPE-CS	879:888	arg1	nanoparticles					893:905	The MSN/TPE-CS@Ag nanoparticles	875:905	The MSN/TPE-CS@Ag nanoparticles	875:905	The MSN/TPE-CS@Ag nanoparticles showed good sensitivity and selectivity for H2O2 even with various interfering ions and agents.					
35926287	0	7	theme	H2O2	91:94	arg1	detection					78:86	the sensitive and selective detection	50:86	the sensitive and selective detection of H2O2	50:94	Chitosan coated fluorescent mesoporous silica for the sensitive and selective detection of H2O2.					
35926287	7	8	theme	optimized	1009:1017	arg1	conditions					1019:1028	optimized conditions	1009:1028	optimized conditions	1009:1028	Under optimized conditions, the detection limit for H2O2 was 0.64 μM in the rage of 1-300 μM.					
35926287	5	9	theme	fluorescence	848:859	arg1	spectroscopy					861:872	fluorescence spectroscopy	848:872	fluorescence spectroscopy	848:872	This fluorescent sensor was characterized with respect to its chemical composition, morphological features and optical properties by means of FTIR, XRD, TGA, SEM, TEM, XPS, UV-Vis and fluorescence spectroscopy.					
35926287	3	10	theme	energy	470:475	arg1	SPEET					487:491	SPEET	487:491	SPEET	487:491	Because of the surface plasmon-enhanced energy transfer (SPEET), AgNCs effectively quenched the fluorescence emission of nanoparticles.					
35926287	3	10	theme	energy	470:475	arg1	transfer					477:484	the surface plasmon-enhanced energy transfer	441:484	the surface plasmon-enhanced energy transfer (SPEET)	441:492	Because of the surface plasmon-enhanced energy transfer (SPEET), AgNCs effectively quenched the fluorescence emission of nanoparticles.					
35926287	2	11	theme	MSNs	351:354	arg1	gatekeeper					380:389	the gatekeeper	376:389	the gatekeeper	376:389	The surface of MSNs was coated by CS as the gatekeeper and the template for loading of AgNCs.					
35926287	2	11	theme	MSNs	351:354	arg1	template					399:406	the template	395:406	the template for loading of AgNCs	395:427	The surface of MSNs was coated by CS as the gatekeeper and the template for loading of AgNCs.					
35926287	2	11	theme	MSNs	351:354	arg1	surface					340:346	The surface	336:346	The surface of MSNs	336:354	The surface of MSNs was coated by CS as the gatekeeper and the template for loading of AgNCs.					
35926287	8	12	from	samples	1216:1222	arg1	quantitative					1182:1193	quantitative	1182:1193	quantitative	1182:1193	The feasibility of the practical application of this probe was confirmed by accurate quantitative of H2O2 in practical samples.					
35926287	6	13	theme	good	914:917	arg1	sensitivity					919:929	good sensitivity	914:929	good sensitivity	914:929	The MSN/TPE-CS@Ag nanoparticles showed good sensitivity and selectivity for H2O2 even with various interfering ions and agents.					
35926287	6	14	theme	various	966:972	arg1	ions					986:989	various interfering ions	966:989	various interfering ions	966:989	The MSN/TPE-CS@Ag nanoparticles showed good sensitivity and selectivity for H2O2 even with various interfering ions and agents.					
35926287	5	15	theme	chemical	726:733	arg1	composition					735:745	its chemical composition	722:745	its chemical composition	722:745	This fluorescent sensor was characterized with respect to its chemical composition, morphological features and optical properties by means of FTIR, XRD, TGA, SEM, TEM, XPS, UV-Vis and fluorescence spectroscopy.					
35926287	5	16	theme	fluorescent	669:679	arg1	sensor					681:686	This fluorescent sensor	664:686	This fluorescent sensor	664:686	This fluorescent sensor was characterized with respect to its chemical composition, morphological features and optical properties by means of FTIR, XRD, TGA, SEM, TEM, XPS, UV-Vis and fluorescence spectroscopy.					
35926287	4	17	theme	fluorescence	650:661	arg1	recovery					638:645	the recovery	634:645	the recovery of fluorescence	634:661	In the presence of H2O2, AgNCs can be oxidized to Ag+, resulting in the recovery of fluorescence.					
35926287	8	18	theme	practical	1120:1128	arg1	application					1130:1140	the practical application	1116:1140	the practical application of this probe	1116:1154	The feasibility of the practical application of this probe was confirmed by accurate quantitative of H2O2 in practical samples.					
35926287	8	19	theme	application	1130:1140	arg1	feasibility					1101:1111	The feasibility	1097:1111	The feasibility of the practical application of this probe	1097:1154	The feasibility of the practical application of this probe was confirmed by accurate quantitative of H2O2 in practical samples.					
35926287	0	20	theme	mesoporous	28:37	arg1	silica					39:44	fluorescent mesoporous silica	16:44	fluorescent mesoporous silica	16:44	Chitosan coated fluorescent mesoporous silica for the sensitive and selective detection of H2O2.					
35926287	1	21	theme	1-	252:253	arg1	-1,2,2-triphenylethene					269:290	1-(4-Aminophenyl)-1,2,2-triphenylethene	252:290	1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2)	252:300	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	1	21	theme	1-	252:253	arg1	TPE-NH2					293:299	TPE-NH2	293:299	TPE-NH2	293:299	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	4	22	dep	H2O2	585:588	arg1	the					569:571	the	569:571	the	569:571	In the presence of H2O2, AgNCs can be oxidized to Ag+, resulting in the recovery of fluorescence.					
35926287	4	22	dep	H2O2	585:588	arg1	presence					573:580	presence	573:580	presence	573:580	In the presence of H2O2, AgNCs can be oxidized to Ag+, resulting in the recovery of fluorescence.					
35926287	8	23	theme	probe	1150:1154	arg1	application					1130:1140	the practical application	1116:1140	the practical application of this probe	1116:1154	The feasibility of the practical application of this probe was confirmed by accurate quantitative of H2O2 in practical samples.					
35926287	0	24	theme	fluorescent	16:26	arg1	silica					39:44	fluorescent mesoporous silica	16:44	fluorescent mesoporous silica	16:44	Chitosan coated fluorescent mesoporous silica for the sensitive and selective detection of H2O2.					
35926287	1	25	theme	novel	99:103	arg1	sensor					125:130	A novel turn-on fluorescent sensor	97:130	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2)	97:159	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	1	26	theme	hydrogen	136:143	arg1	peroxide					145:152	hydrogen peroxide	136:152	hydrogen peroxide (H2O2)	136:159	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	1	26	theme	hydrogen	136:143	arg1	H2O2					155:158	H2O2	155:158	H2O2	155:158	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	3	27	theme	fluorescence	526:537	arg1	emission					539:546	the fluorescence emission	522:546	the fluorescence emission of nanoparticles	522:563	Because of the surface plasmon-enhanced energy transfer (SPEET), AgNCs effectively quenched the fluorescence emission of nanoparticles.					
35926287	5	28	theme	optical	775:781	arg1	properties					783:792	optical properties	775:792	optical properties	775:792	This fluorescent sensor was characterized with respect to its chemical composition, morphological features and optical properties by means of FTIR, XRD, TGA, SEM, TEM, XPS, UV-Vis and fluorescence spectroscopy.					
35926287	6	29	theme	interfering	974:984	arg1	ions					986:989	various interfering ions	966:989	various interfering ions	966:989	The MSN/TPE-CS@Ag nanoparticles showed good sensitivity and selectivity for H2O2 even with various interfering ions and agents.					
35926287	0	30	theme	sensitive	54:62	arg1	detection					78:86	the sensitive and selective detection	50:86	the sensitive and selective detection of H2O2	50:94	Chitosan coated fluorescent mesoporous silica for the sensitive and selective detection of H2O2.					
35926287	1	31	theme	-1,2,2-triphenylethene	269:290	arg1	AgNCs					328:332	AgNCs	328:332	AgNCs	328:332	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	1	31	theme	-1,2,2-triphenylethene	269:290	arg1	nanoparticles					313:325	1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles	252:325	1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs)	252:333	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	1	32	theme	turn-on	105:111	arg1	sensor					125:130	A novel turn-on fluorescent sensor	97:130	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2)	97:159	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	7	33	theme	detection	1035:1043	arg1	limit					1045:1049	the detection limit	1031:1049	the detection limit for H2O2	1031:1058	Under optimized conditions, the detection limit for H2O2 was 0.64 μM in the rage of 1-300 μM.					
35926287	1	34	theme	fluorescent	113:123	arg1	sensor					125:130	A novel turn-on fluorescent sensor	97:130	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2)	97:159	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	5	35	theme	morphological	748:760	arg1	features					762:769	morphological features	748:769	morphological features	748:769	This fluorescent sensor was characterized with respect to its chemical composition, morphological features and optical properties by means of FTIR, XRD, TGA, SEM, TEM, XPS, UV-Vis and fluorescence spectroscopy.					
35926287	8	36	theme	H2O2	1198:1201	arg1	quantitative					1182:1193	quantitative	1182:1193	quantitative	1182:1193	The feasibility of the practical application of this probe was confirmed by accurate quantitative of H2O2 in practical samples.					
35926287	1	37	theme	mesoporous	201:210	arg1	MSNs					234:237	MSNs	234:237	MSNs	234:237	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	1	37	theme	mesoporous	201:210	arg1	nanoparticles					219:231	mesoporous silica nanoparticles	201:231	mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs)	201:333	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	0	38	theme	selective	68:76	arg1	detection					78:86	the sensitive and selective detection	50:86	the sensitive and selective detection of H2O2	50:94	Chitosan coated fluorescent mesoporous silica for the sensitive and selective detection of H2O2.					
35926287	3	39	theme	surface	445:451	arg1	SPEET					487:491	SPEET	487:491	SPEET	487:491	Because of the surface plasmon-enhanced energy transfer (SPEET), AgNCs effectively quenched the fluorescence emission of nanoparticles.					
35926287	3	39	theme	surface	445:451	arg1	transfer					477:484	the surface plasmon-enhanced energy transfer	441:484	the surface plasmon-enhanced energy transfer (SPEET)	441:492	Because of the surface plasmon-enhanced energy transfer (SPEET), AgNCs effectively quenched the fluorescence emission of nanoparticles.					
35926287	1	40	theme	silica	212:217	arg1	MSNs					234:237	MSNs	234:237	MSNs	234:237	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	1	40	theme	silica	212:217	arg1	nanoparticles					219:231	mesoporous silica nanoparticles	201:231	mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs)	201:333	A novel turn-on fluorescent sensor for hydrogen peroxide (H2O2) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH2) and silver nanoparticles (AgNCs).					
35926287	3	41	theme	nanoparticles	551:563	arg1	emission					539:546	the fluorescence emission	522:546	the fluorescence emission of nanoparticles	522:563	Because of the surface plasmon-enhanced energy transfer (SPEET), AgNCs effectively quenched the fluorescence emission of nanoparticles.					
35926287	3	42	theme	plasmon-enhanced	453:468	arg1	SPEET					487:491	SPEET	487:491	SPEET	487:491	Because of the surface plasmon-enhanced energy transfer (SPEET), AgNCs effectively quenched the fluorescence emission of nanoparticles.					
35926287	3	42	theme	plasmon-enhanced	453:468	arg1	transfer					477:484	the surface plasmon-enhanced energy transfer	441:484	the surface plasmon-enhanced energy transfer (SPEET)	441:492	Because of the surface plasmon-enhanced energy transfer (SPEET), AgNCs effectively quenched the fluorescence emission of nanoparticles.					
35926287	7	43	theme	1-300 μM	1087:1094	arg1	rage					1079:1082	the rage	1075:1082	the rage of 1-300 μM	1075:1094	Under optimized conditions, the detection limit for H2O2 was 0.64 μM in the rage of 1-300 μM.					
35700733	3	0	theme	walls	1023:1027	arg1	penetration					991:1001	its penetration	987:1001	its penetration of green algal cell walls	987:1027	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	2	1	theme	biochemical	787:797	arg1	composition					799:809	diverse biochemical composition	779:809	diverse biochemical composition	779:809	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	6	2	theme	enzyme	1576:1581	arg1	exploration					1583:1593	enzyme exploration	1576:1593	enzyme exploration	1576:1593	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases) belong to families that are largely uncharacterized, emphasizing the potential of non-fungal microeukaryotes for enzyme exploration.					
35700733	2	3	theme	well-defined	627:638	arg1	perforations					640:651	well-defined perforations	627:651	well-defined perforations of species-specific size	627:676	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	4	dep	feeders	283:289	arg1	represent					292:300	represent	292:300	represent	292:300	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	6	5	theme	microeukaryotes	1556:1570	arg1	potential					1532:1540	the potential	1528:1540	the potential of non-fungal microeukaryotes for enzyme exploration	1528:1593	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases) belong to families that are largely uncharacterized, emphasizing the potential of non-fungal microeukaryotes for enzyme exploration.					
35700733	7	6	theme	Orciraptor	1701:1710	arg1	agilis					1712:1717	Orciraptor agilis	1701:1717	Orciraptor agilis	1701:1717	Unexpectedly, we discovered various chitin-related factors that point to an unknown chitin metabolism in Orciraptor agilis, potentially also involved in the feeding process.					
35700733	3	7	dep	used	830:833	arg1	Rhizaria					887:894	Rhizaria	887:894	Rhizaria	887:894	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	2	8	theme	mechanistic	316:326	arg1	intermediate					328:339	a fascinating mechanistic intermediate	302:339	a fascinating mechanistic intermediate	302:339	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	7	9	theme	unknown	1672:1678	arg1	metabolism					1687:1696	an unknown chitin metabolism	1669:1696	an unknown chitin metabolism in Orciraptor agilis	1669:1717	Unexpectedly, we discovered various chitin-related factors that point to an unknown chitin metabolism in Orciraptor agilis, potentially also involved in the feeding process.					
35700733	2	10	theme	cell	599:602	arg1	wall					604:607	the prey cell wall	590:607	the prey cell wall	590:607	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	11	theme	protoplast	734:743	arg1	feeders					745:751	protoplast feeders	734:751	protoplast feeders	734:751	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	8	12	theme	microbial	1834:1842	arg1	behavior					1852:1859	an important microbial feeding behavior	1821:1859	an important microbial feeding behavior	1821:1859	Our findings provide first molecular insights into an important microbial feeding behavior and new directions for cell biology research on non-model eukaryotes.					
35700733	3	13	theme	flagellate	839:848	arg1	feeder					919:924	a model protoplast feeder	900:924	a model protoplast feeder	900:924	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	3	13	theme	flagellate	839:848	arg1	agilis					861:866	the flagellate Orciraptor agilis	835:866	the flagellate Orciraptor agilis	835:866	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	2	14	theme	cell	765:768	arg1	walls					770:774	cell walls	765:774	cell walls of diverse biochemical composition	765:809	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	15	theme	unicellular	259:269	arg1	protoplast					272:281	The unicellular "protoplast	255:281	The unicellular "protoplast	255:281	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	8	16	theme	new	1865:1867	arg1	directions					1869:1878	new directions	1865:1878	new directions for cell biology research on non-model eukaryotes	1865:1928	Our findings provide first molecular insights into an important microbial feeding behavior and new directions for cell biology research on non-model eukaryotes.					
35700733	7	17	theme	chitin-related	1632:1645	arg1	factors					1647:1653	various chitin-related factors	1624:1653	various chitin-related factors that point to an unknown chitin metabolism in Orciraptor agilis, potentially also involved in the feeding process	1624:1767	Unexpectedly, we discovered various chitin-related factors that point to an unknown chitin metabolism in Orciraptor agilis, potentially also involved in the feeding process.					
35700733	3	18	used	used	830:833	arg2	We					827:828	We	827:828	We	827:828	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	6	19	theme	Other	1395:1399	arg1	enzymes					1411:1417	Other candidate enzymes	1395:1417	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases)	1395:1461	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases) belong to families that are largely uncharacterized, emphasizing the potential of non-fungal microeukaryotes for enzyme exploration.					
35700733	6	19	theme	Other	1395:1399	arg1	monooxygenases					1447:1460	lytic polysaccharide monooxygenases	1426:1460	lytic polysaccharide monooxygenases	1426:1460	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases) belong to families that are largely uncharacterized, emphasizing the potential of non-fungal microeukaryotes for enzyme exploration.					
35700733	4	20	dep	locomotion	1111:1120	arg1	e.g.					1105:1108	e.g.	1105:1108	e.g.	1105:1108	Besides distinct expression changes that reflect major cellular processes (e.g., locomotion and cell division), we found lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga.					
35700733	1	21	theme	feeding	168:174	arg1	strategies					176:185	feeding strategies	168:185	feeding strategies	168:185	Microbial eukaryotes display a stunning diversity of feeding strategies, ranging from generalist predators to highly specialized parasites.					
35700733	3	22	theme	model	902:906	arg1	feeder					919:924	a model protoplast feeder	900:924	a model protoplast feeder	900:924	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	3	22	theme	model	902:906	arg1	agilis					861:866	the flagellate Orciraptor agilis	835:866	the flagellate Orciraptor agilis	835:866	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	0	23	theme	cell	92:95	arg1	perforation					102:112	cell wall perforation	92:112	cell wall perforation	92:112	Comparative transcriptomics reveals the molecular toolkit used by an algivorous protist for cell wall perforation.					
35700733	8	24	theme	biology	1889:1895	arg1	research					1897:1904	cell biology research	1884:1904	cell biology research on non-model eukaryotes	1884:1928	Our findings provide first molecular insights into an important microbial feeding behavior and new directions for cell biology research on non-model eukaryotes.					
35700733	3	25	theme	gene	951:954	arg1	analysis					967:974	differential gene expression analysis	938:974	differential gene expression analysis	938:974	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	2	26	theme	species-specific	656:671	arg1	size					673:676	species-specific size	656:676	species-specific size	656:676	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	5	27	theme	key	1357:1359	arg1	factor					1361:1366	a potential key factor	1345:1366	a potential key factor for cell wall dissolution	1345:1392	A putative endocellulase (family GH5_5) with a secretion signal is most prominent, and a potential key factor for cell wall dissolution.					
35700733	7	28	from	metabolism	1687:1696	arg1	agilis					1712:1717	Orciraptor agilis	1701:1717	Orciraptor agilis	1701:1717	Unexpectedly, we discovered various chitin-related factors that point to an unknown chitin metabolism in Orciraptor agilis, potentially also involved in the feeding process.					
35700733	4	29	theme	major	1079:1083	arg1	processes					1094:1102	major cellular processes	1079:1102	major cellular processes (e.g., locomotion and cell division)	1079:1139	Besides distinct expression changes that reflect major cellular processes (e.g., locomotion and cell division), we found lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga.					
35700733	2	30	theme	molecular	702:710	arg1	processes					712:720	structure.2 Yet the molecular processes	682:720	structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition	682:809	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	0	31	theme	Comparative	0:10	arg1	transcriptomics					12:26	Comparative transcriptomics	0:26	Comparative transcriptomics	0:26	Comparative transcriptomics reveals the molecular toolkit used by an algivorous protist for cell wall perforation.					
35700733	2	32	theme	cell	443:446	arg1	contents					448:455	their cell contents	437:455	their cell contents	437:455	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	4	33	theme	expression	1047:1056	arg1	changes					1058:1064	distinct expression changes	1038:1064	distinct expression changes that reflect major cellular processes (e.g., locomotion and cell division)	1038:1139	Besides distinct expression changes that reflect major cellular processes (e.g., locomotion and cell division), we found lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga.					
35700733	4	34	theme	carbohydrate-active	1157:1175	arg1	enzymes					1177:1183	lytic carbohydrate-active enzymes	1151:1183	lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga	1151:1255	Besides distinct expression changes that reflect major cellular processes (e.g., locomotion and cell division), we found lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga.					
35700733	6	35	theme	lytic	1426:1430	arg1	enzymes					1411:1417	Other candidate enzymes	1395:1417	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases)	1395:1461	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases) belong to families that are largely uncharacterized, emphasizing the potential of non-fungal microeukaryotes for enzyme exploration.					
35700733	6	35	theme	lytic	1426:1430	arg1	monooxygenases					1447:1460	lytic polysaccharide monooxygenases	1426:1460	lytic polysaccharide monooxygenases	1426:1460	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases) belong to families that are largely uncharacterized, emphasizing the potential of non-fungal microeukaryotes for enzyme exploration.					
35700733	0	36	theme	molecular	40:48	arg1	toolkit					50:56	the molecular toolkit	36:56	the molecular toolkit used by an algivorous protist for cell wall perforation	36:112	Comparative transcriptomics reveals the molecular toolkit used by an algivorous protist for cell wall perforation.					
35700733	4	37	from	attack	1238:1243	arg1	alga					1252:1255	the alga	1248:1255	the alga	1248:1255	Besides distinct expression changes that reflect major cellular processes (e.g., locomotion and cell division), we found lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga.					
35700733	3	38	theme	green	1006:1010	arg1	walls					1023:1027	green algal cell walls	1006:1027	green algal cell walls	1006:1027	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	2	39	theme	eukaryotic	366:375	arg1	fungi					394:398	fungi	394:398	fungi	394:398	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	39	theme	eukaryotic	366:375	arg1	algae					384:388	algae	384:388	algae	384:388	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	39	theme	eukaryotic	366:375	arg1	cells					377:381	other eukaryotic cells	360:381	other eukaryotic cells (algae and fungi) like some parasites	360:419	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	6	40	dep	monooxygenases	1447:1460	arg1	e.g.					1420:1423	e.g.	1420:1423	e.g.	1420:1423	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases) belong to families that are largely uncharacterized, emphasizing the potential of non-fungal microeukaryotes for enzyme exploration.					
35700733	4	41	theme	cell	1126:1129	arg1	division					1131:1138	cell division	1126:1138	cell division	1126:1138	Besides distinct expression changes that reflect major cellular processes (e.g., locomotion and cell division), we found lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga.					
35700733	5	42	theme	putative	1260:1267	arg1	endocellulase					1269:1281	A putative endocellulase	1258:1281	A putative endocellulase (family GH5_5) with a secretion signal	1258:1320	A putative endocellulase (family GH5_5) with a secretion signal is most prominent, and a potential key factor for cell wall dissolution.					
35700733	5	42	theme	putative	1260:1267	arg1	GH5_5					1291:1295	family GH5_5	1284:1295	family GH5_5	1284:1295	A putative endocellulase (family GH5_5) with a secretion signal is most prominent, and a potential key factor for cell wall dissolution.					
35700733	5	42	theme	putative	1260:1267	arg1	prominent					1330:1338	prominent	1330:1338	prominent	1330:1338	A putative endocellulase (family GH5_5) with a secretion signal is most prominent, and a potential key factor for cell wall dissolution.					
35700733	5	43	theme	wall	1377:1380	arg1	dissolution					1382:1392	cell wall dissolution	1372:1392	cell wall dissolution	1372:1392	A putative endocellulase (family GH5_5) with a secretion signal is most prominent, and a potential key factor for cell wall dissolution.					
35700733	3	44	theme	cell	1018:1021	arg1	walls					1023:1027	green algal cell walls	1006:1027	green algal cell walls	1006:1027	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	5	45	with	endocellulase	1269:1281	arg1	signal					1315:1320	a secretion signal	1303:1320	a secretion signal	1303:1320	A putative endocellulase (family GH5_5) with a secretion signal is most prominent, and a potential key factor for cell wall dissolution.					
35700733	2	46	theme	fascinating	304:314	arg1	intermediate					328:339	a fascinating mechanistic intermediate	302:339	a fascinating mechanistic intermediate	302:339	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	47	dep	local	551:555	arg1	pre-phagocytotic					558:573	pre-phagocytotic	558:573	pre-phagocytotic	558:573	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	48	theme	wall	604:607	arg1	dissolution					575:585	the local, pre-phagocytotic dissolution	547:585	the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size	547:676	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	49	theme	composition	799:809	arg1	walls					770:774	cell walls	765:774	cell walls of diverse biochemical composition	765:809	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	1	50	theme	strategies	176:185	arg1	diversity					155:163	a stunning diversity	144:163	a stunning diversity of feeding strategies, ranging from generalist predators to highly specialized parasites	144:252	Microbial eukaryotes display a stunning diversity of feeding strategies, ranging from generalist predators to highly specialized parasites.					
35700733	2	51	theme	diverse	779:785	arg1	composition					799:809	diverse biochemical composition	779:809	diverse biochemical composition	779:809	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	6	52	theme	non-fungal	1545:1554	arg1	microeukaryotes					1556:1570	non-fungal microeukaryotes	1545:1570	non-fungal microeukaryotes	1545:1570	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases) belong to families that are largely uncharacterized, emphasizing the potential of non-fungal microeukaryotes for enzyme exploration.					
35700733	1	53	theme	generalist	201:210	arg1	predators					212:220	generalist predators	201:220	generalist predators	201:220	Microbial eukaryotes display a stunning diversity of feeding strategies, ranging from generalist predators to highly specialized parasites.					
35700733	7	54	theme	chitin	1680:1685	arg1	metabolism					1687:1696	an unknown chitin metabolism	1669:1696	an unknown chitin metabolism in Orciraptor agilis	1669:1717	Unexpectedly, we discovered various chitin-related factors that point to an unknown chitin metabolism in Orciraptor agilis, potentially also involved in the feeding process.					
35700733	4	55	theme	lytic	1151:1155	arg1	enzymes					1177:1183	lytic carbohydrate-active enzymes	1151:1183	lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga	1151:1255	Besides distinct expression changes that reflect major cellular processes (e.g., locomotion and cell division), we found lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga.					
35700733	8	56	theme	first	1791:1795	arg1	insights					1807:1814	first molecular insights	1791:1814	first molecular insights into an important microbial feeding behavior and new directions for cell biology research on non-model eukaryotes	1791:1928	Our findings provide first molecular insights into an important microbial feeding behavior and new directions for cell biology research on non-model eukaryotes.					
35700733	8	57	theme	molecular	1797:1805	arg1	insights					1807:1814	first molecular insights	1791:1814	first molecular insights into an important microbial feeding behavior and new directions for cell biology research on non-model eukaryotes	1791:1928	Our findings provide first molecular insights into an important microbial feeding behavior and new directions for cell biology research on non-model eukaryotes.					
35700733	2	58	theme	local	551:555	arg1	dissolution					575:585	the local, pre-phagocytotic dissolution	547:585	the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size	547:676	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	1	59	theme	Microbial	115:123	arg1	eukaryotes					125:134	Microbial eukaryotes	115:134	Microbial eukaryotes	115:134	Microbial eukaryotes display a stunning diversity of feeding strategies, ranging from generalist predators to highly specialized parasites.					
35700733	2	60	theme	prey	594:597	arg1	wall					604:607	the prey cell wall	590:607	the prey cell wall	590:607	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	5	61	theme	secretion	1305:1313	arg1	signal					1315:1320	a secretion signal	1303:1320	a secretion signal	1303:1320	A putative endocellulase (family GH5_5) with a secretion signal is most prominent, and a potential key factor for cell wall dissolution.					
35700733	8	62	theme	important	1824:1832	arg1	behavior					1852:1859	an important microbial feeding behavior	1821:1859	an important microbial feeding behavior	1821:1859	Our findings provide first molecular insights into an important microbial feeding behavior and new directions for cell biology research on non-model eukaryotes.					
35700733	3	63	theme	Orciraptor	850:859	arg1	feeder					919:924	a model protoplast feeder	900:924	a model protoplast feeder	900:924	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	3	63	theme	Orciraptor	850:859	arg1	agilis					861:866	the flagellate Orciraptor agilis	835:866	the flagellate Orciraptor agilis	835:866	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	0	64	theme	algivorous	69:78	arg1	protist					80:86	an algivorous protist	66:86	an algivorous protist for cell wall perforation	66:112	Comparative transcriptomics reveals the molecular toolkit used by an algivorous protist for cell wall perforation.					
35700733	8	65	theme	feeding	1844:1850	arg1	behavior					1852:1859	an important microbial feeding behavior	1821:1859	an important microbial feeding behavior	1821:1859	Our findings provide first molecular insights into an important microbial feeding behavior and new directions for cell biology research on non-model eukaryotes.					
35700733	1	66	theme	stunning	146:153	arg1	diversity					155:163	a stunning diversity	144:163	a stunning diversity of feeding strategies, ranging from generalist predators to highly specialized parasites	144:252	Microbial eukaryotes display a stunning diversity of feeding strategies, ranging from generalist predators to highly specialized parasites.					
35700733	7	67	theme	various	1624:1630	arg1	factors					1647:1653	various chitin-related factors	1624:1653	various chitin-related factors that point to an unknown chitin metabolism in Orciraptor agilis, potentially also involved in the feeding process	1624:1767	Unexpectedly, we discovered various chitin-related factors that point to an unknown chitin metabolism in Orciraptor agilis, potentially also involved in the feeding process.					
35700733	4	68	theme	distinct	1038:1045	arg1	changes					1058:1064	distinct expression changes	1038:1064	distinct expression changes that reflect major cellular processes (e.g., locomotion and cell division)	1038:1139	Besides distinct expression changes that reflect major cellular processes (e.g., locomotion and cell division), we found lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga.					
35700733	1	69	theme	specialized	232:242	arg1	parasites					244:252	highly specialized parasites	225:252	highly specialized parasites	225:252	Microbial eukaryotes display a stunning diversity of feeding strategies, ranging from generalist predators to highly specialized parasites.					
35700733	6	70	theme	candidate	1401:1409	arg1	enzymes					1411:1417	Other candidate enzymes	1395:1417	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases)	1395:1461	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases) belong to families that are largely uncharacterized, emphasizing the potential of non-fungal microeukaryotes for enzyme exploration.					
35700733	6	70	theme	candidate	1401:1409	arg1	monooxygenases					1447:1460	lytic polysaccharide monooxygenases	1426:1460	lytic polysaccharide monooxygenases	1426:1460	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases) belong to families that are largely uncharacterized, emphasizing the potential of non-fungal microeukaryotes for enzyme exploration.					
35700733	8	71	from	research	1897:1904	arg1	eukaryotes					1919:1928	non-model eukaryotes	1909:1928	non-model eukaryotes	1909:1928	Our findings provide first molecular insights into an important microbial feeding behavior and new directions for cell biology research on non-model eukaryotes.					
35700733	0	72	theme	wall	97:100	arg1	perforation					102:112	cell wall perforation	92:112	cell wall perforation	92:112	Comparative transcriptomics reveals the molecular toolkit used by an algivorous protist for cell wall perforation.					
35700733	3	73	theme	protoplast	908:917	arg1	feeder					919:924	a model protoplast feeder	900:924	a model protoplast feeder	900:924	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	3	73	theme	protoplast	908:917	arg1	agilis					861:866	the flagellate Orciraptor agilis	835:866	the flagellate Orciraptor agilis	835:866	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	2	74	theme	size	673:676	arg1	perforations					640:651	well-defined perforations	627:651	well-defined perforations of species-specific size	627:676	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	75	dep	cells	377:381	arg1	fungi					394:398	fungi	394:398	fungi	394:398	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	75	dep	cells	377:381	arg1	algae					384:388	algae	384:388	algae	384:388	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	75	dep	cells	377:381	arg1	cells					377:381	other eukaryotic cells	360:381	other eukaryotic cells (algae and fungi) like some parasites	360:419	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	5	76	theme	potential	1347:1355	arg1	factor					1361:1366	a potential key factor	1345:1366	a potential key factor for cell wall dissolution	1345:1392	A putative endocellulase (family GH5_5) with a secretion signal is most prominent, and a potential key factor for cell wall dissolution.					
35700733	8	77	theme	cell	1884:1887	arg1	research					1897:1904	cell biology research	1884:1904	cell biology research on non-model eukaryotes	1884:1928	Our findings provide first molecular insights into an important microbial feeding behavior and new directions for cell biology research on non-model eukaryotes.					
35700733	3	78	theme	expression	956:965	arg1	analysis					967:974	differential gene expression analysis	938:974	differential gene expression analysis	938:974	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	2	79	theme	complex	521:527	arg1	behavior					529:536	this complex behavior	516:536	this complex behavior	516:536	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	3	80	theme	differential	938:949	arg1	analysis					967:974	differential gene expression analysis	938:974	differential gene expression analysis	938:974	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	8	81	theme	non-model	1909:1917	arg1	eukaryotes					1919:1928	non-model eukaryotes	1909:1928	non-model eukaryotes	1909:1928	Our findings provide first molecular insights into an important microbial feeding behavior and new directions for cell biology research on non-model eukaryotes.					
35700733	4	82	theme	cellular	1085:1092	arg1	processes					1094:1102	major cellular processes	1079:1102	major cellular processes (e.g., locomotion and cell division)	1079:1139	Besides distinct expression changes that reflect major cellular processes (e.g., locomotion and cell division), we found lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga.					
35700733	6	83	theme	polysaccharide	1432:1445	arg1	enzymes					1411:1417	Other candidate enzymes	1395:1417	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases)	1395:1461	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases) belong to families that are largely uncharacterized, emphasizing the potential of non-fungal microeukaryotes for enzyme exploration.					
35700733	6	83	theme	polysaccharide	1432:1445	arg1	monooxygenases					1447:1460	lytic polysaccharide monooxygenases	1426:1460	lytic polysaccharide monooxygenases	1426:1460	Other candidate enzymes (e.g., lytic polysaccharide monooxygenases) belong to families that are largely uncharacterized, emphasizing the potential of non-fungal microeukaryotes for enzyme exploration.					
35700733	7	84	theme	feeding	1753:1759	arg1	process					1761:1767	the feeding process	1749:1767	the feeding process	1749:1767	Unexpectedly, we discovered various chitin-related factors that point to an unknown chitin metabolism in Orciraptor agilis, potentially also involved in the feeding process.					
35700733	2	85	theme	prey	483:486	arg1	recognition					488:498	prey recognition	483:498	prey recognition	483:498	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	3	86	theme	algal	1012:1016	arg1	walls					1023:1027	green algal cell walls	1006:1027	green algal cell walls	1006:1027	We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder and applied differential gene expression analysis to examine its penetration of green algal cell walls.					
35700733	2	87	theme	other	360:364	arg1	fungi					394:398	fungi	394:398	fungi	394:398	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	87	theme	other	360:364	arg1	algae					384:388	algae	384:388	algae	384:388	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	2	87	theme	other	360:364	arg1	cells					377:381	other eukaryotic cells	360:381	other eukaryotic cells (algae and fungi) like some parasites	360:419	The unicellular "protoplast feeders" represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae and fungi) like some parasites but then devour their cell contents by phagocytosis.1 Besides prey recognition and attachment, this complex behavior involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure.2 Yet the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown.					
35700733	5	88	theme	cell	1372:1375	arg1	dissolution					1382:1392	cell wall dissolution	1372:1392	cell wall dissolution	1372:1392	A putative endocellulase (family GH5_5) with a secretion signal is most prominent, and a potential key factor for cell wall dissolution.					
35700733	5	89	theme	family	1284:1289	arg1	endocellulase					1269:1281	A putative endocellulase	1258:1281	A putative endocellulase (family GH5_5) with a secretion signal	1258:1320	A putative endocellulase (family GH5_5) with a secretion signal is most prominent, and a potential key factor for cell wall dissolution.					
35700733	5	89	theme	family	1284:1289	arg1	GH5_5					1291:1295	family GH5_5	1284:1295	family GH5_5	1284:1295	A putative endocellulase (family GH5_5) with a secretion signal is most prominent, and a potential key factor for cell wall dissolution.					
35429497	3	0	theme	monsoon	456:462	arg1	season					464:469	the monsoon season	452:469	the monsoon season (0.423 particles/L)	452:489	The concentration of MPs was found to be higher during the monsoon season (0.423 particles/L) in comparison with the post-monsoon (0.117 particles/L) period.					
35429497	3	0	theme	monsoon	456:462	arg1	particles/L					478:488	0.423 particles/L	472:488	0.423 particles/L	472:488	The concentration of MPs was found to be higher during the monsoon season (0.423 particles/L) in comparison with the post-monsoon (0.117 particles/L) period.					
35429497	1	1	theme	serious	222:228	arg1	microplastics					172:184	microplastics	172:184	microplastics (MPs) in the lake ecosystems	172:213	Hazardous anthropogenic particles, such as microplastics (MPs) in the lake ecosystems, are a serious concern.					
35429497	1	1	theme	serious	222:228	arg1	particles					153:161	Hazardous anthropogenic particles	129:161	Hazardous anthropogenic particles	129:161	Hazardous anthropogenic particles, such as microplastics (MPs) in the lake ecosystems, are a serious concern.					
35429497	1	1	theme	serious	222:228	arg1	concern					230:236	a serious concern	220:236	a serious concern	220:236	Hazardous anthropogenic particles, such as microplastics (MPs) in the lake ecosystems, are a serious concern.					
35429497	12	2	theme	water	1597:1601	arg1	column					1603:1608	the water column	1593:1608	the water column	1593:1608	During the post-monsoon period, the PLI values decreased, suggesting that MPs in the water column may have settled and mixed with the sediments.					
35429497	10	3	theme	Load	1277:1280	arg1	data					1294:1297	The Pollution Load Index (PLI) data	1263:1297	The Pollution Load Index (PLI) data	1263:1297	The Pollution Load Index (PLI) data reveals that pollution due to microplastics in Lake Manipal falls within the Level I risk category.					
35429497	11	4	theme	monsoon	1429:1435	arg1	season					1437:1442	the monsoon season	1425:1442	the monsoon season due to an increased flux of these particles from the nearby region	1425:1509	The PLI was higher during the monsoon season due to an increased flux of these particles from the nearby region.					
35429497	4	5	theme	storm-water	606:616	arg1	sewers					618:623	storm-water sewers	606:623	storm-water sewers connected to the lake as well as surface runoff during periods of high rainfall	606:703	The higher abundance is attributed to the input of storm-water sewers connected to the lake as well as surface runoff during periods of high rainfall.					
35429497	7	6	theme	microplastics	994:1006	arg1	terephthalate					929:941	Polyethylene terephthalate	916:941	Polyethylene terephthalate (PET)	916:947	Polyethylene terephthalate (PET) was the principal polymer composition of the microplastics, followed by cellulose.					
35429497	7	6	theme	microplastics	994:1006	arg1	composition					975:985	the principal polymer composition	953:985	the principal polymer composition of the microplastics	953:1006	Polyethylene terephthalate (PET) was the principal polymer composition of the microplastics, followed by cellulose.					
35429497	13	7	theme	microorganisms	1763:1776	arg1	types					1725:1729	different types	1715:1729	different types of birds, amphibians, and other microorganisms	1715:1776	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	3	8	theme	post-monsoon	514:525	arg1	period					547:552	the post-monsoon (0.117 particles/L) period	510:552	the post-monsoon (0.117 particles/L) period	510:552	The concentration of MPs was found to be higher during the monsoon season (0.423 particles/L) in comparison with the post-monsoon (0.117 particles/L) period.					
35429497	8	9	theme	clothes	1104:1110	arg1	laundering					1090:1099	the laundering	1086:1099	the laundering of clothes in the residential colonies and hostels situated close to the lake	1086:1177	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	6	10	theme	pellets	889:895	arg1	amounts					867:873	smaller amounts	859:873	smaller amounts of fragments, pellets, films, and foams	859:913	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	10	theme	pellets	889:895	arg1	films					898:902	films	898:902	films	898:902	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	10	theme	pellets	889:895	arg1	foams					909:913	foams	909:913	foams	909:913	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	10	theme	pellets	889:895	arg1	pellets					889:895	pellets	889:895	pellets	889:895	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	10	theme	pellets	889:895	arg1	fragments					878:886	fragments	878:886	fragments	878:886	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	8	11	attach	derived	1073:1079	arg2	fibres					1054:1059	The PET and cellulose fibres	1032:1059	fibres	1054:1059	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	8	11	attach	derived	1073:1079	arg1	laundering					1090:1099	the laundering	1086:1099	the laundering of clothes in the residential colonies and hostels situated close to the lake	1086:1177	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	0	12	theme	Southern	107:114	arg1	Lake					123:126	a Southern Indian Lake	105:126	a Southern Indian Lake	105:126	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	6	13	theme	fragments	878:886	arg1	amounts					867:873	smaller amounts	859:873	smaller amounts of fragments, pellets, films, and foams	859:913	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	13	theme	fragments	878:886	arg1	films					898:902	films	898:902	films	898:902	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	13	theme	fragments	878:886	arg1	foams					909:913	foams	909:913	foams	909:913	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	13	theme	fragments	878:886	arg1	pellets					889:895	pellets	889:895	pellets	889:895	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	13	theme	fragments	878:886	arg1	fragments					878:886	fragments	878:886	fragments	878:886	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	14	14	theme	its	1964:1966	arg1	vicinity					1968:1975	the lake and its vicinity	1951:1975	vicinity	1968:1975	We also propose certain policy measures that can be adopted by the regional population to mitigate microplastic pollution in the lake and its vicinity.					
35429497	0	15	theme	Lake	123:126	arg1	waters					95:100	the surface waters	83:100	the surface waters of a Southern Indian Lake	83:126	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	14	16	theme	lake	1955:1958	arg1	vicinity					1968:1975	the lake and its vicinity	1951:1975	vicinity	1968:1975	We also propose certain policy measures that can be adopted by the regional population to mitigate microplastic pollution in the lake and its vicinity.					
35429497	8	17	dep	colonies	1131:1138	arg1	the					1115:1117	the	1115:1117	the	1115:1117	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	8	18	from	hostels	1144:1150	arg1	laundering					1090:1099	the laundering	1086:1099	the laundering of clothes in the residential colonies and hostels situated close to the lake	1086:1177	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	1	19	from	microplastics	172:184	arg1	ecosystems					204:213	the lake ecosystems	195:213	the lake ecosystems	195:213	Hazardous anthropogenic particles, such as microplastics (MPs) in the lake ecosystems, are a serious concern.					
35429497	13	20	located	present	1782:1788	arg2	types					1725:1729	different types	1715:1729	different types of birds, amphibians, and other microorganisms	1715:1776	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	13	20	located	present	1782:1788	arg1	environment					1797:1807	the environment	1793:1807	the environment of Lake Manipal	1793:1823	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	0	21	theme	microplastic	57:68	arg1	particles					70:78	microplastic particles	57:78	microplastic particles	57:78	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	1	22	theme	anthropogenic	139:151	arg1	microplastics					172:184	microplastics	172:184	microplastics (MPs) in the lake ecosystems	172:213	Hazardous anthropogenic particles, such as microplastics (MPs) in the lake ecosystems, are a serious concern.					
35429497	1	22	theme	anthropogenic	139:151	arg1	concern					230:236	a serious concern	220:236	a serious concern	220:236	Hazardous anthropogenic particles, such as microplastics (MPs) in the lake ecosystems, are a serious concern.					
35429497	1	22	theme	anthropogenic	139:151	arg1	particles					153:161	Hazardous anthropogenic particles	129:161	Hazardous anthropogenic particles	129:161	Hazardous anthropogenic particles, such as microplastics (MPs) in the lake ecosystems, are a serious concern.					
35429497	2	23	from	India	390:394	arg1	samples					353:359	the surface water samples	335:359	the surface water samples of Lake Manipal in southwest India	335:394	In this work, we have investigated the seasonal occurrence and distribution of microplastics in the surface water samples of Lake Manipal in southwest India.					
35429497	6	24	theme	microplastics	820:832	arg1	%					811:811	Approximately 96%	795:811	Approximately 96% of the microplastics	795:832	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	24	theme	microplastics	820:832	arg1	microplastics					820:832	the microplastics	816:832	the microplastics	816:832	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	3	25	dep	post-monsoon	514:525	arg1	particles/L					534:544	0.117 particles/L	528:544	0.117 particles/L	528:544	The concentration of MPs was found to be higher during the monsoon season (0.423 particles/L) in comparison with the post-monsoon (0.117 particles/L) period.					
35429497	12	26	theme	PLI	1548:1550	arg1	values					1552:1557	the PLI values	1544:1557	the PLI values	1544:1557	During the post-monsoon period, the PLI values decreased, suggesting that MPs in the water column may have settled and mixed with the sediments.					
35429497	4	27	theme	high	691:694	arg1	rainfall					696:703	high rainfall	691:703	high rainfall	691:703	The higher abundance is attributed to the input of storm-water sewers connected to the lake as well as surface runoff during periods of high rainfall.					
35429497	0	28	theme	surface	87:93	arg1	waters					95:100	the surface waters	83:100	the surface waters of a Southern Indian Lake	83:126	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	6	29	theme	films	898:902	arg1	amounts					867:873	smaller amounts	859:873	smaller amounts of fragments, pellets, films, and foams	859:913	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	29	theme	films	898:902	arg1	films					898:902	films	898:902	films	898:902	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	29	theme	films	898:902	arg1	foams					909:913	foams	909:913	foams	909:913	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	29	theme	films	898:902	arg1	pellets					889:895	pellets	889:895	pellets	889:895	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	29	theme	films	898:902	arg1	fragments					878:886	fragments	878:886	fragments	878:886	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	0	30	from	distribution	41:52	arg1	waters					95:100	the surface waters	83:100	the surface waters of a Southern Indian Lake	83:126	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	12	31	theme	post-monsoon	1523:1534	arg1	period					1536:1541	the post-monsoon period	1519:1541	the post-monsoon period	1519:1541	During the post-monsoon period, the PLI values decreased, suggesting that MPs in the water column may have settled and mixed with the sediments.					
35429497	0	32	from	abundance	27:35	arg1	waters					95:100	the surface waters	83:100	the surface waters of a Southern Indian Lake	83:126	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	2	33	dep	occurrence	287:296	arg1	the					274:276	the	274:276	the	274:276	In this work, we have investigated the seasonal occurrence and distribution of microplastics in the surface water samples of Lake Manipal in southwest India.					
35429497	2	34	theme	water	347:351	arg1	samples					353:359	the surface water samples	335:359	the surface water samples of Lake Manipal in southwest India	335:394	In this work, we have investigated the seasonal occurrence and distribution of microplastics in the surface water samples of Lake Manipal in southwest India.					
35429497	0	35	theme	Seasonal	0:7	arg1	variations					9:18	Seasonal variations	0:18	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.	0:127	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	10	36	theme	Level	1376:1380	arg1	category					1389:1396	the Level I risk category	1372:1396	the Level I risk category	1372:1396	The Pollution Load Index (PLI) data reveals that pollution due to microplastics in Lake Manipal falls within the Level I risk category.					
35429497	2	37	theme	southwest	380:388	arg1	India					390:394	southwest India	380:394	southwest India	380:394	In this work, we have investigated the seasonal occurrence and distribution of microplastics in the surface water samples of Lake Manipal in southwest India.					
35429497	5	38	theme	microplastics	751:763	arg1	concentrations					710:723	The concentrations	706:723	The concentrations of small-sized (0.3-1 mm) microplastics	706:763	The concentrations of small-sized (0.3-1 mm) microplastics were greater in both seasons.					
35429497	5	38	theme	microplastics	751:763	arg1	greater					770:776	greater	770:776	greater	770:776	The concentrations of small-sized (0.3-1 mm) microplastics were greater in both seasons.					
35429497	2	39	theme	Manipal	369:375	arg1	samples					353:359	the surface water samples	335:359	the surface water samples of Lake Manipal in southwest India	335:394	In this work, we have investigated the seasonal occurrence and distribution of microplastics in the surface water samples of Lake Manipal in southwest India.					
35429497	11	40	theme	increased	1454:1462	arg1	flux					1464:1467	an increased flux	1451:1467	an increased flux of these particles from the nearby region	1451:1509	The PLI was higher during the monsoon season due to an increased flux of these particles from the nearby region.					
35429497	5	41	theme	small-sized	728:738	arg1	0.3-1 mm					741:748	0.3-1 mm	741:748	0.3-1 mm	741:748	The concentrations of small-sized (0.3-1 mm) microplastics were greater in both seasons.					
35429497	5	41	theme	small-sized	728:738	arg1	microplastics					751:763	small-sized (0.3-1 mm) microplastics	728:763	small-sized (0.3-1 mm) microplastics	728:763	The concentrations of small-sized (0.3-1 mm) microplastics were greater in both seasons.					
35429497	0	42	dep	abundance	27:35	arg1	the					23:25	the	23:25	the	23:25	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	10	43	theme	risk	1384:1387	arg1	category					1389:1396	the Level I risk category	1372:1396	the Level I risk category	1372:1396	The Pollution Load Index (PLI) data reveals that pollution due to microplastics in Lake Manipal falls within the Level I risk category.					
35429497	1	44	theme	lake	199:202	arg1	ecosystems					204:213	the lake ecosystems	195:213	the lake ecosystems	195:213	Hazardous anthropogenic particles, such as microplastics (MPs) in the lake ecosystems, are a serious concern.					
35429497	13	45	from	environment	1797:1807	arg1	present					1782:1788	present	1782:1788	present	1782:1788	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	11	46	theme	particles	1478:1486	arg1	flux					1464:1467	an increased flux	1451:1467	an increased flux of these particles from the nearby region	1451:1509	The PLI was higher during the monsoon season due to an increased flux of these particles from the nearby region.					
35429497	8	47	theme	PET	1036:1038	arg1	fibres					1054:1059	The PET and cellulose fibres	1032:1059	fibres	1054:1059	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	8	48	from	clothes	1104:1110	arg1	hostels					1144:1150	hostels	1144:1150	hostels	1144:1150	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	8	48	from	clothes	1104:1110	arg1	colonies					1131:1138	colonies	1131:1138	colonies	1131:1138	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	2	49	theme	microplastics	318:330	arg1	distribution					302:313	distribution	302:313	distribution	302:313	In this work, we have investigated the seasonal occurrence and distribution of microplastics in the surface water samples of Lake Manipal in southwest India.					
35429497	2	49	theme	microplastics	318:330	arg1	occurrence					287:296	seasonal occurrence	278:296	seasonal occurrence	278:296	In this work, we have investigated the seasonal occurrence and distribution of microplastics in the surface water samples of Lake Manipal in southwest India.					
35429497	8	50	theme	cellulose	1044:1052	arg1	fibres					1054:1059	The PET and cellulose fibres	1032:1059	fibres	1054:1059	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	8	51	from	laundering	1090:1099	arg1	hostels					1144:1150	hostels	1144:1150	hostels	1144:1150	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	8	51	from	laundering	1090:1099	arg1	colonies					1131:1138	colonies	1131:1138	colonies	1131:1138	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	2	52	from	occurrence	287:296	arg1	samples					353:359	the surface water samples	335:359	the surface water samples of Lake Manipal in southwest India	335:394	In this work, we have investigated the seasonal occurrence and distribution of microplastics in the surface water samples of Lake Manipal in southwest India.					
35429497	10	53	theme	Index	1282:1286	arg1	data					1294:1297	The Pollution Load Index (PLI) data	1263:1297	The Pollution Load Index (PLI) data	1263:1297	The Pollution Load Index (PLI) data reveals that pollution due to microplastics in Lake Manipal falls within the Level I risk category.					
35429497	8	54	from	colonies	1131:1138	arg1	laundering					1090:1099	the laundering	1086:1099	the laundering of clothes in the residential colonies and hostels situated close to the lake	1086:1177	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	2	55	from	distribution	302:313	arg1	samples					353:359	the surface water samples	335:359	the surface water samples of Lake Manipal in southwest India	335:394	In this work, we have investigated the seasonal occurrence and distribution of microplastics in the surface water samples of Lake Manipal in southwest India.					
35429497	10	56	theme	Pollution	1267:1275	arg1	data					1294:1297	The Pollution Load Index (PLI) data	1263:1297	The Pollution Load Index (PLI) data	1263:1297	The Pollution Load Index (PLI) data reveals that pollution due to microplastics in Lake Manipal falls within the Level I risk category.					
35429497	11	57	from	region	1504:1509	arg1	flux					1464:1467	an increased flux	1451:1467	an increased flux of these particles from the nearby region	1451:1509	The PLI was higher during the monsoon season due to an increased flux of these particles from the nearby region.					
35429497	13	58	theme	other	1757:1761	arg1	microorganisms					1763:1776	other microorganisms	1757:1776	other microorganisms	1757:1776	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	10	59	from	pollution	1312:1320	arg1	Manipal					1351:1357	Manipal	1351:1357	Manipal	1351:1357	The Pollution Load Index (PLI) data reveals that pollution due to microplastics in Lake Manipal falls within the Level I risk category.					
35429497	8	60	theme	situated	1152:1159	arg1	colonies					1131:1138	colonies	1131:1138	colonies	1131:1138	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	0	61	theme	Indian	116:121	arg1	Lake					123:126	a Southern Indian Lake	105:126	a Southern Indian Lake	105:126	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	10	62	theme	PLI	1289:1291	arg1	data					1294:1297	The Pollution Load Index (PLI) data	1263:1297	The Pollution Load Index (PLI) data	1263:1297	The Pollution Load Index (PLI) data reveals that pollution due to microplastics in Lake Manipal falls within the Level I risk category.					
35429497	7	63	theme	principal	957:965	arg1	terephthalate					929:941	Polyethylene terephthalate	916:941	Polyethylene terephthalate (PET)	916:947	Polyethylene terephthalate (PET) was the principal polymer composition of the microplastics, followed by cellulose.					
35429497	7	63	theme	principal	957:965	arg1	composition					975:985	the principal polymer composition	953:985	the principal polymer composition of the microplastics	953:1006	Polyethylene terephthalate (PET) was the principal polymer composition of the microplastics, followed by cellulose.					
35429497	8	64	theme	residential	1119:1129	arg1	colonies					1131:1138	colonies	1131:1138	colonies	1131:1138	The PET and cellulose fibres were mainly derived from the laundering of clothes in the residential colonies and hostels situated close to the lake.					
35429497	6	65	theme	smaller	859:865	arg1	amounts					867:873	smaller amounts	859:873	smaller amounts of fragments, pellets, films, and foams	859:913	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	65	theme	smaller	859:865	arg1	films					898:902	films	898:902	films	898:902	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	65	theme	smaller	859:865	arg1	foams					909:913	foams	909:913	foams	909:913	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	65	theme	smaller	859:865	arg1	pellets					889:895	pellets	889:895	pellets	889:895	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	65	theme	smaller	859:865	arg1	fragments					878:886	fragments	878:886	fragments	878:886	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	13	66	theme	birds	1734:1738	arg1	types					1725:1729	different types	1715:1729	different types of birds, amphibians, and other microorganisms	1715:1776	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	7	67	theme	Polyethylene	916:927	arg1	composition					975:985	the principal polymer composition	953:985	the principal polymer composition of the microplastics	953:1006	Polyethylene terephthalate (PET) was the principal polymer composition of the microplastics, followed by cellulose.					
35429497	7	67	theme	Polyethylene	916:927	arg1	PET					944:946	PET	944:946	PET	944:946	Polyethylene terephthalate (PET) was the principal polymer composition of the microplastics, followed by cellulose.					
35429497	7	67	theme	Polyethylene	916:927	arg1	terephthalate					929:941	Polyethylene terephthalate	916:941	Polyethylene terephthalate (PET)	916:947	Polyethylene terephthalate (PET) was the principal polymer composition of the microplastics, followed by cellulose.					
35429497	13	68	theme	amphibians	1741:1750	arg1	types					1725:1729	different types	1715:1729	different types of birds, amphibians, and other microorganisms	1715:1776	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	0	69	theme	particles	70:78	arg1	distribution					41:52	distribution	41:52	distribution	41:52	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	0	69	theme	particles	70:78	arg1	abundance					27:35	abundance	27:35	abundance	27:35	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	1	70	theme	Hazardous	129:137	arg1	microplastics					172:184	microplastics	172:184	microplastics (MPs) in the lake ecosystems	172:213	Hazardous anthropogenic particles, such as microplastics (MPs) in the lake ecosystems, are a serious concern.					
35429497	1	70	theme	Hazardous	129:137	arg1	concern					230:236	a serious concern	220:236	a serious concern	220:236	Hazardous anthropogenic particles, such as microplastics (MPs) in the lake ecosystems, are a serious concern.					
35429497	1	70	theme	Hazardous	129:137	arg1	particles					153:161	Hazardous anthropogenic particles	129:161	Hazardous anthropogenic particles	129:161	Hazardous anthropogenic particles, such as microplastics (MPs) in the lake ecosystems, are a serious concern.					
35429497	4	71	theme	sewers	618:623	arg1	input					597:601	the input	593:601	the input of storm-water sewers connected to the lake as well as surface runoff during periods of high rainfall	593:703	The higher abundance is attributed to the input of storm-water sewers connected to the lake as well as surface runoff during periods of high rainfall.					
35429497	9	72	theme	storm-water	1184:1194	arg1	sewers					1196:1201	The storm-water sewers	1180:1201	The storm-water sewers	1180:1201	The storm-water sewers were the likely conduit for these PET fibres into the lake.					
35429497	9	72	theme	storm-water	1184:1194	arg1	conduit					1219:1225	the likely conduit	1208:1225	the likely conduit for these PET fibres into the lake	1208:1260	The storm-water sewers were the likely conduit for these PET fibres into the lake.					
35429497	14	73	theme	policy	1850:1855	arg1	measures					1857:1864	certain policy measures	1842:1864	certain policy measures that can be adopted by the regional population to mitigate microplastic pollution in the lake and its vicinity	1842:1975	We also propose certain policy measures that can be adopted by the regional population to mitigate microplastic pollution in the lake and its vicinity.					
35429497	13	74	from	present	1782:1788	arg1	environment					1797:1807	the environment	1793:1807	the environment of Lake Manipal	1793:1823	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	6	75	theme	foams	909:913	arg1	amounts					867:873	smaller amounts	859:873	smaller amounts of fragments, pellets, films, and foams	859:913	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	75	theme	foams	909:913	arg1	films					898:902	films	898:902	films	898:902	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	75	theme	foams	909:913	arg1	foams					909:913	foams	909:913	foams	909:913	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	75	theme	foams	909:913	arg1	pellets					889:895	pellets	889:895	pellets	889:895	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	6	75	theme	foams	909:913	arg1	fragments					878:886	fragments	878:886	fragments	878:886	Approximately 96% of the microplastics were fibres, followed by smaller amounts of fragments, pellets, films, and foams.					
35429497	0	76	from	variations	9:18	arg1	distribution					41:52	distribution	41:52	distribution	41:52	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	0	76	from	variations	9:18	arg1	abundance					27:35	abundance	27:35	abundance	27:35	Seasonal variations in the abundance and distribution of microplastic particles in the surface waters of a Southern Indian Lake.					
35429497	14	77	theme	certain	1842:1848	arg1	measures					1857:1864	certain policy measures	1842:1864	certain policy measures that can be adopted by the regional population to mitigate microplastic pollution in the lake and its vicinity	1842:1975	We also propose certain policy measures that can be adopted by the regional population to mitigate microplastic pollution in the lake and its vicinity.					
35429497	14	78	theme	microplastic	1925:1936	arg1	pollution					1938:1946	microplastic pollution	1925:1946	microplastic pollution	1925:1946	We also propose certain policy measures that can be adopted by the regional population to mitigate microplastic pollution in the lake and its vicinity.					
35429497	9	79	theme	likely	1212:1217	arg1	sewers					1196:1201	The storm-water sewers	1180:1201	The storm-water sewers	1180:1201	The storm-water sewers were the likely conduit for these PET fibres into the lake.					
35429497	9	79	theme	likely	1212:1217	arg1	conduit					1219:1225	the likely conduit	1208:1225	the likely conduit for these PET fibres into the lake	1208:1260	The storm-water sewers were the likely conduit for these PET fibres into the lake.					
35429497	13	80	theme	different	1715:1723	arg1	types					1725:1729	different types	1715:1729	different types of birds, amphibians, and other microorganisms	1715:1776	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	4	81	theme	rainfall	696:703	arg1	periods					680:686	periods	680:686	periods of high rainfall	680:703	The higher abundance is attributed to the input of storm-water sewers connected to the lake as well as surface runoff during periods of high rainfall.					
35429497	3	82	with	comparison	494:503	arg1	period					547:552	the post-monsoon (0.117 particles/L) period	510:552	the post-monsoon (0.117 particles/L) period	510:552	The concentration of MPs was found to be higher during the monsoon season (0.423 particles/L) in comparison with the post-monsoon (0.117 particles/L) period.					
35429497	14	83	theme	regional	1893:1900	arg1	population					1902:1911	the regional population	1889:1911	the regional population	1889:1911	We also propose certain policy measures that can be adopted by the regional population to mitigate microplastic pollution in the lake and its vicinity.					
35429497	13	84	theme	baseline	1661:1668	arg1	data					1670:1673	The baseline data	1657:1673	The baseline data generated in this study	1657:1697	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	13	84	theme	baseline	1661:1668	arg1	important					1702:1710	important	1702:1710	important	1702:1710	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	2	85	theme	surface	339:345	arg1	samples					353:359	the surface water samples	335:359	the surface water samples of Lake Manipal in southwest India	335:394	In this work, we have investigated the seasonal occurrence and distribution of microplastics in the surface water samples of Lake Manipal in southwest India.					
35429497	9	86	theme	PET	1237:1239	arg1	fibres					1241:1246	these PET fibres	1231:1246	these PET fibres	1231:1246	The storm-water sewers were the likely conduit for these PET fibres into the lake.					
35429497	12	87	from	MPs	1586:1588	arg1	column					1603:1608	the water column	1593:1608	the water column	1593:1608	During the post-monsoon period, the PLI values decreased, suggesting that MPs in the water column may have settled and mixed with the sediments.					
35429497	3	88	theme	MPs	418:420	arg1	higher					438:443	higher	438:443	higher	438:443	The concentration of MPs was found to be higher during the monsoon season (0.423 particles/L) in comparison with the post-monsoon (0.117 particles/L) period.					
35429497	3	88	theme	MPs	418:420	arg1	concentration					401:413	The concentration	397:413	The concentration of MPs	397:420	The concentration of MPs was found to be higher during the monsoon season (0.423 particles/L) in comparison with the post-monsoon (0.117 particles/L) period.					
35429497	7	89	theme	polymer	967:973	arg1	terephthalate					929:941	Polyethylene terephthalate	916:941	Polyethylene terephthalate (PET)	916:947	Polyethylene terephthalate (PET) was the principal polymer composition of the microplastics, followed by cellulose.					
35429497	7	89	theme	polymer	967:973	arg1	composition					975:985	the principal polymer composition	953:985	the principal polymer composition of the microplastics	953:1006	Polyethylene terephthalate (PET) was the principal polymer composition of the microplastics, followed by cellulose.					
35429497	11	90	theme	due	1444:1446	arg1	season					1437:1442	the monsoon season	1425:1442	the monsoon season due to an increased flux of these particles from the nearby region	1425:1509	The PLI was higher during the monsoon season due to an increased flux of these particles from the nearby region.					
35429497	2	91	from	samples	353:359	arg1	India					390:394	southwest India	380:394	southwest India	380:394	In this work, we have investigated the seasonal occurrence and distribution of microplastics in the surface water samples of Lake Manipal in southwest India.					
35429497	10	92	theme	I	1382:1382	arg1	category					1389:1396	the Level I risk category	1372:1396	the Level I risk category	1372:1396	The Pollution Load Index (PLI) data reveals that pollution due to microplastics in Lake Manipal falls within the Level I risk category.					
35429497	2	93	theme	seasonal	278:285	arg1	occurrence					287:296	seasonal occurrence	278:296	seasonal occurrence	278:296	In this work, we have investigated the seasonal occurrence and distribution of microplastics in the surface water samples of Lake Manipal in southwest India.					
35429497	13	94	attach	present	1782:1788	arg1	environment					1797:1807	the environment	1793:1807	the environment of Lake Manipal	1793:1823	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	13	94	attach	present	1782:1788	arg2	types					1725:1729	different types	1715:1729	different types of birds, amphibians, and other microorganisms	1715:1776	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
35429497	4	95	theme	higher	559:564	arg1	abundance					566:574	The higher abundance	555:574	The higher abundance	555:574	The higher abundance is attributed to the input of storm-water sewers connected to the lake as well as surface runoff during periods of high rainfall.					
35429497	11	96	theme	nearby	1497:1502	arg1	region					1504:1509	the nearby region	1493:1509	the nearby region	1493:1509	The PLI was higher during the monsoon season due to an increased flux of these particles from the nearby region.					
35429497	13	97	theme	Manipal	1817:1823	arg1	environment					1797:1807	the environment	1793:1807	the environment of Lake Manipal	1793:1823	The baseline data generated in this study is important as different types of birds, amphibians, and other microorganisms are present in the environment of Lake Manipal.					
36448728	1	0	theme	energy	320:325	arg1	homeostasis					327:337	the host energy homeostasis	311:337	the host energy homeostasis	311:337	Obesity is a major risk factor for the development of type 2 diabetes and cardiovascular diseases, and gut microbiota plays a key role in influencing the host energy homeostasis.					
36448728	6	1	theme	layer	1539:1543	arg1	markers					1518:1524	markers	1518:1524	markers of the mucus layer	1518:1543	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	5	2	from	changes	1169:1175	arg1	composition					1199:1209	the gut microbiota composition	1180:1209	the gut microbiota composition	1180:1209	These results were associated with significant changes in the gut microbiota composition, with FOS significantly increasing the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae.					
36448728	5	3	theme	gut	1184:1186	arg1	composition					1199:1209	the gut microbiota composition	1180:1209	the gut microbiota composition	1180:1209	These results were associated with significant changes in the gut microbiota composition, with FOS significantly increasing the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae.					
36448728	1	4	theme	diseases	250:257	arg1	development					200:210	the development	196:210	the development of type 2 diabetes and cardiovascular diseases	196:257	Obesity is a major risk factor for the development of type 2 diabetes and cardiovascular diseases, and gut microbiota plays a key role in influencing the host energy homeostasis.					
36448728	0	5	theme	gut	87:89	arg1	microbiota					91:100	the gut microbiota	83:100	the gut microbiota	83:100	Prebiotic oligofructose protects against high-fat diet-induced obesity by changing the gut microbiota, intestinal mucus production, glycosylation and secretion.					
36448728	0	5	theme	gut	87:89	arg1	secretion					150:158	secretion	150:158	secretion	150:158	Prebiotic oligofructose protects against high-fat diet-induced obesity by changing the gut microbiota, intestinal mucus production, glycosylation and secretion.					
36448728	0	5	theme	gut	87:89	arg1	glycosylation					132:144	glycosylation	132:144	glycosylation	132:144	Prebiotic oligofructose protects against high-fat diet-induced obesity by changing the gut microbiota, intestinal mucus production, glycosylation and secretion.					
36448728	0	5	theme	gut	87:89	arg1	production					120:129	intestinal mucus production	103:129	intestinal mucus production	103:129	Prebiotic oligofructose protects against high-fat diet-induced obesity by changing the gut microbiota, intestinal mucus production, glycosylation and secretion.					
36448728	5	6	theme	Ruminococcaceae	1374:1388	arg1	abundance					1272:1280	the relative and absolute abundance	1246:1280	the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae	1246:1388	These results were associated with significant changes in the gut microbiota composition, with FOS significantly increasing the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae.					
36448728	5	7	theme	microbiota	1188:1197	arg1	composition					1199:1209	the gut microbiota composition	1180:1209	the gut microbiota composition	1180:1209	These results were associated with significant changes in the gut microbiota composition, with FOS significantly increasing the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae.					
36448728	0	8	theme	intestinal	103:112	arg1	microbiota					91:100	the gut microbiota	83:100	the gut microbiota	83:100	Prebiotic oligofructose protects against high-fat diet-induced obesity by changing the gut microbiota, intestinal mucus production, glycosylation and secretion.					
36448728	0	8	theme	intestinal	103:112	arg1	production					120:129	intestinal mucus production	103:129	intestinal mucus production	103:129	Prebiotic oligofructose protects against high-fat diet-induced obesity by changing the gut microbiota, intestinal mucus production, glycosylation and secretion.					
36448728	6	9	with	association	1448:1458	arg1	parameters					1475:1484	metabolic parameters	1465:1484	metabolic parameters	1465:1484	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	6	9	with	association	1448:1458	arg1	markers					1518:1524	markers	1518:1524	markers of the mucus layer	1518:1543	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	7	10	theme	mucus	1689:1693	arg1	production					1695:1704	the mucus production	1685:1704	the mucus production	1685:1704	Our study shows that FOS treatment is able to prevent HFD-induced metabolic disorders, at least in part, by acting on all the processes of the mucus production.					
36448728	3	11	dep	together	660:667	arg1	with					669:672	with	669:672	with	669:672	We previously demonstrated that prebiotic treatment with oligofructose (FOS) counteracted the effects of diet-induced obesity, together with changes in the gut microbiota composition, but it is not known if the intestinal mucus layer could be involved.					
36448728	3	12	with	treatment	575:583	arg1	FOS					605:607	FOS	605:607	FOS	605:607	We previously demonstrated that prebiotic treatment with oligofructose (FOS) counteracted the effects of diet-induced obesity, together with changes in the gut microbiota composition, but it is not known if the intestinal mucus layer could be involved.					
36448728	3	12	with	treatment	575:583	arg1	oligofructose					590:602	oligofructose	590:602	oligofructose (FOS)	590:608	We previously demonstrated that prebiotic treatment with oligofructose (FOS) counteracted the effects of diet-induced obesity, together with changes in the gut microbiota composition, but it is not known if the intestinal mucus layer could be involved.					
36448728	6	13	theme	positive	1492:1499	arg1	association					1501:1511	a positive association	1490:1511	a positive association with markers of the mucus layer	1490:1543	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	7	14	theme	metabolic	1612:1620	arg1	disorders					1622:1630	HFD-induced metabolic disorders	1600:1630	HFD-induced metabolic disorders	1600:1630	Our study shows that FOS treatment is able to prevent HFD-induced metabolic disorders, at least in part, by acting on all the processes of the mucus production.					
36448728	6	15	theme	metabolic	1465:1473	arg1	parameters					1475:1484	metabolic parameters	1465:1484	metabolic parameters	1465:1484	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	4	16	theme	mucins	1062:1067	arg1	number					1098:1103	number	1098:1103	number	1098:1103	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	4	16	theme	mucins	1062:1067	arg1	expression					924:933	the expression	920:933	the expression of numerous genes involved in mucus production, glycosylation and secretion	920:1009	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	4	16	theme	mucins	1062:1067	arg1	differentiation					1078:1092	differentiation	1078:1092	differentiation	1078:1092	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	4	16	theme	mucins	1062:1067	arg1	expression					1016:1025	the expression	1012:1025	the expression of both secreted and transmembrane mucins	1012:1067	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	1	17	theme	gut	264:266	arg1	microbiota					268:277	gut microbiota	264:277	gut microbiota	264:277	Obesity is a major risk factor for the development of type 2 diabetes and cardiovascular diseases, and gut microbiota plays a key role in influencing the host energy homeostasis.					
36448728	5	18	theme	absolute	1263:1270	arg1	abundance					1272:1280	the relative and absolute abundance	1246:1280	the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae	1246:1388	These results were associated with significant changes in the gut microbiota composition, with FOS significantly increasing the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae.					
36448728	6	19	theme	bacterial	1416:1424	arg1	genera					1426:1431	all these bacterial genera	1406:1431	all these bacterial genera	1406:1431	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	4	20	theme	transmembrane	1048:1060	arg1	mucins					1062:1067	both secreted and transmembrane mucins	1030:1067	mucins	1062:1067	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	1	21	theme	major	174:178	arg1	factor					185:190	a major risk factor	172:190	a major risk factor for the development of type 2 diabetes and cardiovascular diseases	172:257	Obesity is a major risk factor for the development of type 2 diabetes and cardiovascular diseases, and gut microbiota plays a key role in influencing the host energy homeostasis.					
36448728	1	21	theme	major	174:178	arg1	Obesity					161:167	Obesity	161:167	Obesity	161:167	Obesity is a major risk factor for the development of type 2 diabetes and cardiovascular diseases, and gut microbiota plays a key role in influencing the host energy homeostasis.					
36448728	0	22	theme	mucus	114:118	arg1	microbiota					91:100	the gut microbiota	83:100	the gut microbiota	83:100	Prebiotic oligofructose protects against high-fat diet-induced obesity by changing the gut microbiota, intestinal mucus production, glycosylation and secretion.					
36448728	0	22	theme	mucus	114:118	arg1	production					120:129	intestinal mucus production	103:129	intestinal mucus production	103:129	Prebiotic oligofructose protects against high-fat diet-induced obesity by changing the gut microbiota, intestinal mucus production, glycosylation and secretion.					
36448728	3	23	theme	microbiota	693:702	arg1	composition					704:714	the gut microbiota composition	685:714	the gut microbiota composition	685:714	We previously demonstrated that prebiotic treatment with oligofructose (FOS) counteracted the effects of diet-induced obesity, together with changes in the gut microbiota composition, but it is not known if the intestinal mucus layer could be involved.					
36448728	2	24	theme	layer	460:464	arg1	alteration					425:434	an alteration	422:434	an alteration of the intestinal mucus layer	422:464	Moreover, obese mice have a different gut microbiota composition, associated with an alteration of the intestinal mucus layer, which represents the interface between the bacteria and the host.					
36448728	7	25	theme	production	1695:1704	arg1	processes					1672:1680	all the processes	1664:1680	all the processes of the mucus production	1664:1704	Our study shows that FOS treatment is able to prevent HFD-induced metabolic disorders, at least in part, by acting on all the processes of the mucus production.					
36448728	4	26	theme	genes	947:951	arg1	number					1098:1103	number	1098:1103	number	1098:1103	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	4	26	theme	genes	947:951	arg1	expression					924:933	the expression	920:933	the expression of numerous genes involved in mucus production, glycosylation and secretion	920:1009	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	4	26	theme	genes	947:951	arg1	differentiation					1078:1092	differentiation	1078:1092	differentiation	1078:1092	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	4	26	theme	genes	947:951	arg1	expression					1016:1025	the expression	1012:1025	the expression of both secreted and transmembrane mucins	1012:1067	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	4	27	dep	differentiation	1078:1092	arg1	the					1074:1076	the	1074:1076	the	1074:1076	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	1	28	theme	risk	180:183	arg1	factor					185:190	a major risk factor	172:190	a major risk factor for the development of type 2 diabetes and cardiovascular diseases	172:257	Obesity is a major risk factor for the development of type 2 diabetes and cardiovascular diseases, and gut microbiota plays a key role in influencing the host energy homeostasis.					
36448728	1	28	theme	risk	180:183	arg1	Obesity					161:167	Obesity	161:167	Obesity	161:167	Obesity is a major risk factor for the development of type 2 diabetes and cardiovascular diseases, and gut microbiota plays a key role in influencing the host energy homeostasis.					
36448728	4	29	dep	preventing	831:840	arg1	addition					819:826	addition	819:826	addition	819:826	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	6	30	theme	mucus	1533:1537	arg1	layer					1539:1543	the mucus layer	1529:1543	the mucus layer	1529:1543	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	0	31	theme	Prebiotic	0:8	arg1	oligofructose					10:22	Prebiotic oligofructose	0:22	Prebiotic oligofructose	0:22	Prebiotic oligofructose protects against high-fat diet-induced obesity by changing the gut microbiota, intestinal mucus production, glycosylation and secretion.					
36448728	3	32	from	changes	674:680	arg1	composition					704:714	the gut microbiota composition	685:714	the gut microbiota composition	685:714	We previously demonstrated that prebiotic treatment with oligofructose (FOS) counteracted the effects of diet-induced obesity, together with changes in the gut microbiota composition, but it is not known if the intestinal mucus layer could be involved.					
36448728	4	33	theme	mucus	965:969	arg1	production					971:980	mucus production	965:980	mucus production	965:980	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	6	34	contain	had	1433:1435	arg2	association					1448:1458	a negative association	1437:1458	a negative association with metabolic parameters	1437:1484	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	6	34	contain	had	1433:1435	arg1	genera					1426:1431	all these bacterial genera	1406:1431	all these bacterial genera	1406:1431	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	6	34	contain	had	1433:1435	arg2	association					1501:1511	a positive association	1490:1511	a positive association with markers of the mucus layer	1490:1543	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	5	35	theme	unknown	1366:1372	arg1	Ruminococcaceae					1374:1388	an unknown Ruminococcaceae	1363:1388	an unknown Ruminococcaceae	1363:1388	These results were associated with significant changes in the gut microbiota composition, with FOS significantly increasing the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae.					
36448728	7	36	theme	HFD-induced	1600:1610	arg1	disorders					1622:1630	HFD-induced metabolic disorders	1600:1630	HFD-induced metabolic disorders	1600:1630	Our study shows that FOS treatment is able to prevent HFD-induced metabolic disorders, at least in part, by acting on all the processes of the mucus production.					
36448728	2	37	contain	have	361:364	arg2	composition					393:403	a different gut microbiota composition	366:403	a different gut microbiota composition	366:403	Moreover, obese mice have a different gut microbiota composition, associated with an alteration of the intestinal mucus layer, which represents the interface between the bacteria and the host.					
36448728	2	37	contain	have	361:364	arg1	mice					356:359	obese mice	350:359	obese mice	350:359	Moreover, obese mice have a different gut microbiota composition, associated with an alteration of the intestinal mucus layer, which represents the interface between the bacteria and the host.					
36448728	5	38	dep	genera	1299:1304	arg1	Muribaculaceae					1344:1357	Muribaculaceae	1344:1357	Muribaculaceae	1344:1357	These results were associated with significant changes in the gut microbiota composition, with FOS significantly increasing the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae.					
36448728	5	38	dep	genera	1299:1304	arg1	Akkermansia					1319:1329	Akkermansia	1319:1329	Akkermansia	1319:1329	These results were associated with significant changes in the gut microbiota composition, with FOS significantly increasing the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae.					
36448728	5	39	theme	relative	1250:1257	arg1	abundance					1272:1280	the relative and absolute abundance	1246:1280	the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae	1246:1388	These results were associated with significant changes in the gut microbiota composition, with FOS significantly increasing the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae.					
36448728	0	40	theme	diet-induced	50:61	arg1	obesity					63:69	high-fat diet-induced obesity	41:69	high-fat diet-induced obesity	41:69	Prebiotic oligofructose protects against high-fat diet-induced obesity by changing the gut microbiota, intestinal mucus production, glycosylation and secretion.					
36448728	1	41	theme	key	287:289	arg1	role					291:294	a key role	285:294	a key role	285:294	Obesity is a major risk factor for the development of type 2 diabetes and cardiovascular diseases, and gut microbiota plays a key role in influencing the host energy homeostasis.					
36448728	3	42	theme	diet-induced	638:649	arg1	obesity					651:657	diet-induced obesity	638:657	diet-induced obesity	638:657	We previously demonstrated that prebiotic treatment with oligofructose (FOS) counteracted the effects of diet-induced obesity, together with changes in the gut microbiota composition, but it is not known if the intestinal mucus layer could be involved.					
36448728	4	43	theme	secreted	1035:1042	arg1	mucins					1062:1067	both secreted and transmembrane mucins	1030:1067	mucins	1062:1067	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	0	44	theme	high-fat	41:48	arg1	obesity					63:69	high-fat diet-induced obesity	41:69	high-fat diet-induced obesity	41:69	Prebiotic oligofructose protects against high-fat diet-induced obesity by changing the gut microbiota, intestinal mucus production, glycosylation and secretion.					
36448728	3	45	theme	intestinal	744:753	arg1	layer					761:765	the intestinal mucus layer	740:765	the intestinal mucus layer	740:765	We previously demonstrated that prebiotic treatment with oligofructose (FOS) counteracted the effects of diet-induced obesity, together with changes in the gut microbiota composition, but it is not known if the intestinal mucus layer could be involved.					
36448728	2	46	theme	mucus	454:458	arg1	layer					460:464	the intestinal mucus layer	439:464	the intestinal mucus layer	439:464	Moreover, obese mice have a different gut microbiota composition, associated with an alteration of the intestinal mucus layer, which represents the interface between the bacteria and the host.					
36448728	4	47	theme	goblet	1108:1113	arg1	cells					1115:1119	goblet cells	1108:1119	goblet cells	1108:1119	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	1	48	theme	type	215:218	arg1	diabetes					222:229	type 2 diabetes	215:229	type 2 diabetes	215:229	Obesity is a major risk factor for the development of type 2 diabetes and cardiovascular diseases, and gut microbiota plays a key role in influencing the host energy homeostasis.					
36448728	2	49	theme	intestinal	443:452	arg1	layer					460:464	the intestinal mucus layer	439:464	the intestinal mucus layer	439:464	Moreover, obese mice have a different gut microbiota composition, associated with an alteration of the intestinal mucus layer, which represents the interface between the bacteria and the host.					
36448728	3	50	theme	mucus	755:759	arg1	layer					761:765	the intestinal mucus layer	740:765	the intestinal mucus layer	740:765	We previously demonstrated that prebiotic treatment with oligofructose (FOS) counteracted the effects of diet-induced obesity, together with changes in the gut microbiota composition, but it is not known if the intestinal mucus layer could be involved.					
36448728	4	51	theme	high-fat	842:849	arg1	diet					851:854	high-fat diet	842:854	high-fat diet (HFD) induced obesity in mice	842:884	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	4	51	theme	high-fat	842:849	arg1	HFD					857:859	HFD	857:859	HFD	857:859	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	3	52	theme	prebiotic	565:573	arg1	treatment					575:583	prebiotic treatment	565:583	prebiotic treatment with oligofructose (FOS)	565:608	We previously demonstrated that prebiotic treatment with oligofructose (FOS) counteracted the effects of diet-induced obesity, together with changes in the gut microbiota composition, but it is not known if the intestinal mucus layer could be involved.					
36448728	6	53	with	association	1501:1511	arg1	parameters					1475:1484	metabolic parameters	1465:1484	metabolic parameters	1465:1484	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	6	53	with	association	1501:1511	arg1	markers					1518:1524	markers	1518:1524	markers of the mucus layer	1518:1543	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	2	54	theme	gut	378:380	arg1	composition					393:403	a different gut microbiota composition	366:403	a different gut microbiota composition	366:403	Moreover, obese mice have a different gut microbiota composition, associated with an alteration of the intestinal mucus layer, which represents the interface between the bacteria and the host.					
36448728	8	55	theme	related	1840:1846	arg1	disorders					1848:1856	related disorders	1840:1856	related disorders	1840:1856	These data suggest that targeting the mucus and the gut microbiota by using prebiotics could help to prevent or mitigate obesity and related disorders.					
36448728	7	56	from	able	1584:1587	arg1	part					1645:1648	part	1645:1648	part	1645:1648	Our study shows that FOS treatment is able to prevent HFD-induced metabolic disorders, at least in part, by acting on all the processes of the mucus production.					
36448728	5	57	theme	significant	1157:1167	arg1	changes					1169:1175	significant changes	1157:1175	significant changes in the gut microbiota composition	1157:1209	These results were associated with significant changes in the gut microbiota composition, with FOS significantly increasing the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae.					
36448728	8	58	theme	gut	1759:1761	arg1	microbiota					1763:1772	the gut microbiota	1755:1772	the gut microbiota	1755:1772	These data suggest that targeting the mucus and the gut microbiota by using prebiotics could help to prevent or mitigate obesity and related disorders.					
36448728	4	59	theme	cells	1115:1119	arg1	number					1098:1103	number	1098:1103	number	1098:1103	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	4	59	theme	cells	1115:1119	arg1	expression					924:933	the expression	920:933	the expression of numerous genes involved in mucus production, glycosylation and secretion	920:1009	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	4	59	theme	cells	1115:1119	arg1	differentiation					1078:1092	differentiation	1078:1092	differentiation	1078:1092	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	4	59	theme	cells	1115:1119	arg1	expression					1016:1025	the expression	1012:1025	the expression of both secreted and transmembrane mucins	1012:1067	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	6	60	theme	negative	1439:1446	arg1	association					1448:1458	a negative association	1437:1458	a negative association with metabolic parameters	1437:1484	Interestingly, all these bacterial genera had a negative association with metabolic parameters and a positive association with markers of the mucus layer.					
36448728	4	61	with	treatment	891:899	arg1	FOS					906:908	FOS	906:908	FOS	906:908	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	3	62	theme	obesity	651:657	arg1	effects					627:633	the effects	623:633	the effects of diet-induced obesity	623:657	We previously demonstrated that prebiotic treatment with oligofructose (FOS) counteracted the effects of diet-induced obesity, together with changes in the gut microbiota composition, but it is not known if the intestinal mucus layer could be involved.					
36448728	5	63	theme	bacterial	1289:1297	arg1	genera					1299:1304	the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae	1285:1388	genera	1299:1304	These results were associated with significant changes in the gut microbiota composition, with FOS significantly increasing the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae.					
36448728	1	64	theme	diabetes	222:229	arg1	development					200:210	the development	196:210	the development of type 2 diabetes and cardiovascular diseases	196:257	Obesity is a major risk factor for the development of type 2 diabetes and cardiovascular diseases, and gut microbiota plays a key role in influencing the host energy homeostasis.					
36448728	2	65	theme	microbiota	382:391	arg1	composition					393:403	a different gut microbiota composition	366:403	a different gut microbiota composition	366:403	Moreover, obese mice have a different gut microbiota composition, associated with an alteration of the intestinal mucus layer, which represents the interface between the bacteria and the host.					
36448728	5	66	theme	genera	1299:1304	arg1	abundance					1272:1280	the relative and absolute abundance	1246:1280	the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae	1246:1388	These results were associated with significant changes in the gut microbiota composition, with FOS significantly increasing the relative and absolute abundance of the bacterial genera Odoribacter, Akkermansia, two unknown Muribaculaceae and an unknown Ruminococcaceae.					
36448728	2	67	theme	different	368:376	arg1	composition					393:403	a different gut microbiota composition	366:403	a different gut microbiota composition	366:403	Moreover, obese mice have a different gut microbiota composition, associated with an alteration of the intestinal mucus layer, which represents the interface between the bacteria and the host.					
36448728	4	68	theme	numerous	938:945	arg1	genes					947:951	numerous genes	938:951	numerous genes involved in mucus production, glycosylation and secretion	938:1009	In this study, we found that, in addition to preventing high-fat diet (HFD) induced obesity in mice, the treatment with FOS increased the expression of numerous genes involved in mucus production, glycosylation and secretion, the expression of both secreted and transmembrane mucins, and the differentiation and number of goblet cells.					
36448728	1	69	theme	host	315:318	arg1	homeostasis					327:337	the host energy homeostasis	311:337	the host energy homeostasis	311:337	Obesity is a major risk factor for the development of type 2 diabetes and cardiovascular diseases, and gut microbiota plays a key role in influencing the host energy homeostasis.					
36448728	7	70	from	part	1645:1648	arg1	able					1584:1587	able	1584:1587	able	1584:1587	Our study shows that FOS treatment is able to prevent HFD-induced metabolic disorders, at least in part, by acting on all the processes of the mucus production.					
36448728	3	71	theme	gut	689:691	arg1	composition					704:714	the gut microbiota composition	685:714	the gut microbiota composition	685:714	We previously demonstrated that prebiotic treatment with oligofructose (FOS) counteracted the effects of diet-induced obesity, together with changes in the gut microbiota composition, but it is not known if the intestinal mucus layer could be involved.					
36448728	2	72	theme	obese	350:354	arg1	mice					356:359	obese mice	350:359	obese mice	350:359	Moreover, obese mice have a different gut microbiota composition, associated with an alteration of the intestinal mucus layer, which represents the interface between the bacteria and the host.					
36448728	7	73	theme	FOS	1567:1569	arg1	treatment					1571:1579	FOS treatment	1567:1579	FOS treatment	1567:1579	Our study shows that FOS treatment is able to prevent HFD-induced metabolic disorders, at least in part, by acting on all the processes of the mucus production.					
36448728	1	74	theme	cardiovascular	235:248	arg1	diseases					250:257	cardiovascular diseases	235:257	cardiovascular diseases	235:257	Obesity is a major risk factor for the development of type 2 diabetes and cardiovascular diseases, and gut microbiota plays a key role in influencing the host energy homeostasis.					
35151707	5	0	theme	composite	756:764	arg1	scaffold					766:773	This composite scaffold	751:773	This composite scaffold	751:773	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	5	1	theme	YAP	1113:1115	arg1	pathway					1128:1134	the yes-associated protein (YAP) signaling pathway	1085:1134	the yes-associated protein (YAP) signaling pathway	1085:1134	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	7	2	theme	Sprague-Dawley	1453:1466	arg1	rats					1468:1471	Sprague-Dawley rats	1453:1471	Sprague-Dawley rats	1453:1471	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	4	3	theme	@	693:693	arg1	scaffolds					697:705	CS/PCL@AL scaffolds	687:705	CS/PCL@AL scaffolds	687:705	Additionally, we explored the possible molecular mechanism of CS/PCL@AL scaffolds in the osteogenic differentiation of EMSCs.					
35151707	1	4	theme	calvarial	275:283	arg1	defect					285:290	a critical-sized calvarial defect	258:290	a critical-sized calvarial defect	258:290	Here, we evaluated osteogenic differentiation in vitro and new bone formation in vivo using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect.					
35151707	2	5	theme	release	466:472	arg1	system					474:479	an AL sustained release system	450:479	an AL sustained release system	450:479	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	8	6	theme	low-cost	1490:1497	arg1	technology					1499:1508	This simple and low-cost technology	1474:1508	This simple and low-cost technology	1474:1508	This simple and low-cost technology may represent a promising strategy to construct an efficient delivery system to repair bone defects.					
35151707	4	7	theme	CS/PCL	687:692	arg1	scaffolds					697:705	CS/PCL@AL scaffolds	687:705	CS/PCL@AL scaffolds	687:705	Additionally, we explored the possible molecular mechanism of CS/PCL@AL scaffolds in the osteogenic differentiation of EMSCs.					
35151707	7	8	theme	@	1341:1341	arg1	scaffold					1345:1352	the CS/PCL@AL scaffold	1331:1352	the CS/PCL@AL scaffold	1331:1352	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	5	9	theme	signaling	1118:1126	arg1	pathway					1128:1134	the yes-associated protein (YAP) signaling pathway	1085:1134	the yes-associated protein (YAP) signaling pathway	1085:1134	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	2	10	dep	sustained	456:464	arg1	AL					453:454	AL	453:454	AL	453:454	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	5	11	theme	@	1009:1009	arg1	scaffold					1013:1020	the CS/PCL@AL scaffold	999:1020	the CS/PCL@AL scaffold	999:1020	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	2	12	theme	genipin	315:321	arg1	action					305:310	the action	301:310	the action	301:310	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	6	13	from	mediators	1196:1204	arg1	differentiation					1224:1238	the osteogenic differentiation	1209:1238	the osteogenic differentiation of EMSCs	1209:1247	YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs.					
35151707	7	14	theme	micro-computed	1259:1272	arg1	tomography					1274:1283	micro-computed tomography	1259:1283	micro-computed tomography analyses	1259:1292	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	6	15	theme	target	1160:1165	arg1	mediators					1196:1204	crucial mediators	1188:1204	crucial mediators	1188:1204	YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs.					
35151707	6	15	theme	target	1160:1165	arg1	YAP					1137:1139	YAP	1137:1139	YAP	1137:1139	YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs.					
35151707	6	15	theme	target	1160:1165	arg1	transglutaminase					1167:1182	its downstream target transglutaminase	1145:1182	its downstream target transglutaminase	1145:1182	YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs.					
35151707	5	16	theme	morphogenetic	928:940	arg1	protein					942:948	bone morphogenetic protein 2	923:950	bone morphogenetic protein 2	923:950	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	5	17	theme	osteogenic	880:889	arg1	differentiation					891:905	EMSC osteogenic differentiation	875:905	EMSC osteogenic differentiation	875:905	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	1	18	theme	bone	150:153	arg1	formation					155:163	new bone formation	146:163	new bone formation	146:163	Here, we evaluated osteogenic differentiation in vitro and new bone formation in vivo using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect.					
35151707	8	19	theme	promising	1526:1534	arg1	strategy					1536:1543	a promising strategy	1524:1543	a promising strategy to construct an efficient delivery system to repair bone defects	1524:1608	This simple and low-cost technology may represent a promising strategy to construct an efficient delivery system to repair bone defects.					
35151707	3	20	theme	AL	510:511	arg1	scaffolds					513:521	CS/PCL@AL scaffolds	503:521	CS/PCL@AL scaffolds	503:521	We demonstrated that CS/PCL@AL scaffolds significantly enhanced the osteogenic differentiation of ectomesenchymal stem cells (EMSCs) in vitro.					
35151707	4	21	theme	molecular	664:672	arg1	mechanism					674:682	the possible molecular mechanism	651:682	the possible molecular mechanism of CS/PCL@AL scaffolds	651:705	Additionally, we explored the possible molecular mechanism of CS/PCL@AL scaffolds in the osteogenic differentiation of EMSCs.					
35151707	2	22	theme	CS/PCL	404:409	arg1	scaffold					421:428	the CS/PCL composite scaffold	400:428	the CS/PCL composite scaffold (CS/PCL@AL)	400:440	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	2	22	theme	CS/PCL	404:409	arg1	@					437:437	CS/PCL@	431:437	CS/PCL@AL	431:439	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	5	23	theme	yes-associated	1089:1102	arg1	pathway					1128:1134	the yes-associated protein (YAP) signaling pathway	1085:1134	the yes-associated protein (YAP) signaling pathway	1085:1134	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	0	24	theme	bone	63:66	arg1	defects					68:74	bone defects	63:74	bone defects repairing	63:84	Alendronate crosslinked chitosan/polycaprolactone scaffold for bone defects repairing.					
35151707	7	25	theme	skull	1431:1435	arg1	defects					1442:1448	skull bone defects	1431:1448	skull bone defects in Sprague-Dawley rats	1431:1471	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	1	26	theme	alendronate-loaded	182:199	arg1	CS/PCL					237:242	CS/PCL	237:242	CS/PCL	237:242	Here, we evaluated osteogenic differentiation in vitro and new bone formation in vivo using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect.					
35151707	1	26	theme	alendronate-loaded	182:199	arg1	scaffold					227:234	an alendronate-loaded chitosan/polycaprolactone scaffold	179:234	an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect	179:290	Here, we evaluated osteogenic differentiation in vitro and new bone formation in vivo using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect.					
35151707	5	27	theme	protein	1104:1110	arg1	pathway					1128:1134	the yes-associated protein (YAP) signaling pathway	1085:1134	the yes-associated protein (YAP) signaling pathway	1085:1134	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	5	28	dep	exerted	775:781	arg1	enhanced					866:873	enhanced	866:873	enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression	866:989	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	5	28	dep	exerted	775:781	arg1	1					840:840	1	840:840	1	840:840	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	5	28	dep	exerted	775:781	arg1	promoted					1022:1029	promoted	1022:1029	promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway	1022:1134	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	7	29	theme	bone	1413:1416	arg1	formation					1418:1426	new and mature bone formation	1398:1426	new and mature bone formation	1398:1426	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	7	30	theme	tomography	1274:1283	arg1	analyses					1285:1292	micro-computed tomography analyses	1259:1292	micro-computed tomography analyses	1259:1292	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	1	31	theme	chitosan/polycaprolactone	201:225	arg1	CS/PCL					237:242	CS/PCL	237:242	CS/PCL	237:242	Here, we evaluated osteogenic differentiation in vitro and new bone formation in vivo using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect.					
35151707	1	31	theme	chitosan/polycaprolactone	201:225	arg1	scaffold					227:234	an alendronate-loaded chitosan/polycaprolactone scaffold	179:234	an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect	179:290	Here, we evaluated osteogenic differentiation in vitro and new bone formation in vivo using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect.					
35151707	5	32	theme	EMSC	807:810	arg1	differentiation					823:837	EMSC osteogenic differentiation	807:837	EMSC osteogenic differentiation	807:837	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	8	33	theme	efficient	1561:1569	arg1	system					1580:1585	an efficient delivery system	1558:1585	an efficient delivery system to repair bone defects	1558:1608	This simple and low-cost technology may represent a promising strategy to construct an efficient delivery system to repair bone defects.					
35151707	5	34	theme	osteogenic	1035:1044	arg1	differentiation					1046:1060	the osteogenic differentiation	1031:1060	the osteogenic differentiation of EMSCs	1031:1069	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	1	35	theme	osteogenic	106:115	arg1	differentiation					117:131	osteogenic differentiation	106:131	osteogenic differentiation	106:131	Here, we evaluated osteogenic differentiation in vitro and new bone formation in vivo using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect.					
35151707	4	36	theme	possible	655:662	arg1	mechanism					674:682	the possible molecular mechanism	651:682	the possible molecular mechanism of CS/PCL@AL scaffolds	651:705	Additionally, we explored the possible molecular mechanism of CS/PCL@AL scaffolds in the osteogenic differentiation of EMSCs.					
35151707	5	37	theme	osteogenic	812:821	arg1	differentiation					823:837	EMSC osteogenic differentiation	807:837	EMSC osteogenic differentiation	807:837	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	4	38	theme	osteogenic	714:723	arg1	differentiation					725:739	the osteogenic differentiation	710:739	the osteogenic differentiation of EMSCs	710:748	Additionally, we explored the possible molecular mechanism of CS/PCL@AL scaffolds in the osteogenic differentiation of EMSCs.					
35151707	5	39	theme	AL	1010:1011	arg1	scaffold					1013:1020	the CS/PCL@AL scaffold	999:1020	the CS/PCL@AL scaffold	999:1020	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	2	40	dep	alendronate	359:369	arg1	AL					372:373	AL	372:373	AL	372:373	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	2	41	theme	CS/PCL	431:436	arg1	scaffold					421:428	the CS/PCL composite scaffold	400:428	the CS/PCL composite scaffold (CS/PCL@AL)	400:440	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	2	41	theme	CS/PCL	431:436	arg1	@					437:437	CS/PCL@	431:437	CS/PCL@AL	431:439	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	0	42	theme	chitosan/polycaprolactone	24:48	arg1	scaffold					50:57	chitosan/polycaprolactone scaffold	24:57	chitosan/polycaprolactone scaffold for bone defects repairing	24:84	Alendronate crosslinked chitosan/polycaprolactone scaffold for bone defects repairing.					
35151707	5	43	theme	laminin	972:978	arg1	expression					980:989	laminin expression	972:989	laminin expression	972:989	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	3	44	theme	osteogenic	550:559	arg1	differentiation					561:575	the osteogenic differentiation	546:575	the osteogenic differentiation of ectomesenchymal stem cells (EMSCs)	546:613	We demonstrated that CS/PCL@AL scaffolds significantly enhanced the osteogenic differentiation of ectomesenchymal stem cells (EMSCs) in vitro.					
35151707	7	45	theme	superior	1366:1373	arg1	capacity					1375:1382	a superior capacity	1364:1382	a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats	1364:1471	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	2	46	theme	sustained	456:464	arg1	system					474:479	an AL sustained release system	450:479	an AL sustained release system	450:479	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	8	47	theme	delivery	1571:1578	arg1	system					1580:1585	an efficient delivery system	1558:1585	an efficient delivery system to repair bone defects	1558:1608	This simple and low-cost technology may represent a promising strategy to construct an efficient delivery system to repair bone defects.					
35151707	6	48	theme	osteogenic	1213:1222	arg1	differentiation					1224:1238	the osteogenic differentiation	1209:1238	the osteogenic differentiation of EMSCs	1209:1247	YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs.					
35151707	3	49	theme	cells	601:605	arg1	differentiation					561:575	the osteogenic differentiation	546:575	the osteogenic differentiation of ectomesenchymal stem cells (EMSCs)	546:613	We demonstrated that CS/PCL@AL scaffolds significantly enhanced the osteogenic differentiation of ectomesenchymal stem cells (EMSCs) in vitro.					
35151707	7	50	theme	CS/PCL	1335:1340	arg1	scaffold					1345:1352	the CS/PCL@AL scaffold	1331:1352	the CS/PCL@AL scaffold	1331:1352	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	7	51	theme	histology	1298:1306	arg1	results					1308:1314	histology results	1298:1314	histology results	1298:1314	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	5	52	theme	CS/PCL	847:852	arg1	scaffold					857:864	the CS/PCL@AL scaffold	843:864	the CS/PCL@AL scaffold	843:864	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	8	53	theme	repair	1590:1595	arg1	defects					1602:1608	repair bone defects	1590:1608	repair bone defects	1590:1608	This simple and low-cost technology may represent a promising strategy to construct an efficient delivery system to repair bone defects.					
35151707	5	54	theme	AL	854:855	arg1	scaffold					857:864	the CS/PCL@AL scaffold	843:864	the CS/PCL@AL scaffold	843:864	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	5	55	theme	EMSCs	1065:1069	arg1	differentiation					1046:1060	the osteogenic differentiation	1031:1060	the osteogenic differentiation of EMSCs	1031:1069	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	5	56	theme	CS/PCL	1003:1008	arg1	scaffold					1013:1020	the CS/PCL@AL scaffold	999:1020	the CS/PCL@AL scaffold	999:1020	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	2	57	theme	composite	411:419	arg1	scaffold					421:428	the CS/PCL composite scaffold	400:428	the CS/PCL composite scaffold (CS/PCL@AL)	400:440	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	2	57	theme	composite	411:419	arg1	@					437:437	CS/PCL@	431:437	CS/PCL@AL	431:439	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	5	58	theme	positive	787:794	arg1	effects					796:802	two positive effects	783:802	two positive effects	783:802	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	3	59	theme	ectomesenchymal	580:594	arg1	cells					601:605	ectomesenchymal stem cells	580:605	ectomesenchymal stem cells (EMSCs)	580:613	We demonstrated that CS/PCL@AL scaffolds significantly enhanced the osteogenic differentiation of ectomesenchymal stem cells (EMSCs) in vitro.					
35151707	3	59	theme	ectomesenchymal	580:594	arg1	EMSCs					608:612	EMSCs	608:612	EMSCs	608:612	We demonstrated that CS/PCL@AL scaffolds significantly enhanced the osteogenic differentiation of ectomesenchymal stem cells (EMSCs) in vitro.					
35151707	4	60	theme	scaffolds	697:705	arg1	mechanism					674:682	the possible molecular mechanism	651:682	the possible molecular mechanism of CS/PCL@AL scaffolds	651:705	Additionally, we explored the possible molecular mechanism of CS/PCL@AL scaffolds in the osteogenic differentiation of EMSCs.					
35151707	2	61	contain	has	330:332	arg1	action					305:310	the action	301:310	the action	301:310	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	2	61	contain	has	330:332	arg2	function					349:356	a crosslinking function	334:356	a crosslinking function	334:356	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	8	62	theme	simple	1479:1484	arg1	technology					1499:1508	This simple and low-cost technology	1474:1508	This simple and low-cost technology	1474:1508	This simple and low-cost technology may represent a promising strategy to construct an efficient delivery system to repair bone defects.					
35151707	3	63	theme	stem	596:599	arg1	cells					601:605	ectomesenchymal stem cells	580:605	ectomesenchymal stem cells (EMSCs)	580:613	We demonstrated that CS/PCL@AL scaffolds significantly enhanced the osteogenic differentiation of ectomesenchymal stem cells (EMSCs) in vitro.					
35151707	3	63	theme	stem	596:599	arg1	EMSCs					608:612	EMSCs	608:612	EMSCs	608:612	We demonstrated that CS/PCL@AL scaffolds significantly enhanced the osteogenic differentiation of ectomesenchymal stem cells (EMSCs) in vitro.					
35151707	7	64	theme	AL	1342:1343	arg1	scaffold					1345:1352	the CS/PCL@AL scaffold	1331:1352	the CS/PCL@AL scaffold	1331:1352	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	4	65	theme	AL	694:695	arg1	scaffolds					697:705	CS/PCL@AL scaffolds	687:705	CS/PCL@AL scaffolds	687:705	Additionally, we explored the possible molecular mechanism of CS/PCL@AL scaffolds in the osteogenic differentiation of EMSCs.					
35151707	8	66	theme	bone	1597:1600	arg1	defects					1602:1608	repair bone defects	1590:1608	repair bone defects	1590:1608	This simple and low-cost technology may represent a promising strategy to construct an efficient delivery system to repair bone defects.					
35151707	6	67	theme	downstream	1149:1158	arg1	mediators					1196:1204	crucial mediators	1188:1204	crucial mediators	1188:1204	YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs.					
35151707	6	67	theme	downstream	1149:1158	arg1	YAP					1137:1139	YAP	1137:1139	YAP	1137:1139	YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs.					
35151707	6	67	theme	downstream	1149:1158	arg1	transglutaminase					1167:1182	its downstream target transglutaminase	1145:1182	its downstream target transglutaminase	1145:1182	YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs.					
35151707	7	68	theme	new	1398:1400	arg1	formation					1418:1426	new and mature bone formation	1398:1426	new and mature bone formation	1398:1426	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	1	69	theme	new	146:148	arg1	formation					155:163	new bone formation	146:163	new bone formation	146:163	Here, we evaluated osteogenic differentiation in vitro and new bone formation in vivo using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect.					
35151707	4	70	theme	EMSCs	744:748	arg1	differentiation					725:739	the osteogenic differentiation	710:739	the osteogenic differentiation of EMSCs	710:748	Additionally, we explored the possible molecular mechanism of CS/PCL@AL scaffolds in the osteogenic differentiation of EMSCs.					
35151707	7	71	theme	mature	1406:1411	arg1	formation					1418:1426	new and mature bone formation	1398:1426	new and mature bone formation	1398:1426	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	7	72	from	defects	1442:1448	arg1	rats					1468:1471	Sprague-Dawley rats	1453:1471	Sprague-Dawley rats	1453:1471	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	7	73	theme	bone	1437:1440	arg1	defects					1442:1448	skull bone defects	1431:1448	skull bone defects in Sprague-Dawley rats	1431:1471	Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats.					
35151707	2	74	theme	crosslinking	336:347	arg1	function					349:356	a crosslinking function	334:356	a crosslinking function	334:356	Through the action of genipin, which has a crosslinking function, alendronate (AL) was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL) to form an AL sustained release system.					
35151707	5	75	theme	bone	923:926	arg1	protein					942:948	bone morphogenetic protein 2	923:950	bone morphogenetic protein 2	923:950	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	5	76	theme	EMSC	875:878	arg1	differentiation					891:905	EMSC osteogenic differentiation	875:905	EMSC osteogenic differentiation	875:905	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	6	77	theme	crucial	1188:1194	arg1	mediators					1196:1204	crucial mediators	1188:1204	crucial mediators	1188:1204	YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs.					
35151707	6	77	theme	crucial	1188:1194	arg1	YAP					1137:1139	YAP	1137:1139	YAP	1137:1139	YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs.					
35151707	6	77	theme	crucial	1188:1194	arg1	transglutaminase					1167:1182	its downstream target transglutaminase	1145:1182	its downstream target transglutaminase	1145:1182	YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs.					
35151707	1	78	from	scaffold	227:234	arg1	rats					248:251	rats	248:251	rats with a critical-sized calvarial defect	248:290	Here, we evaluated osteogenic differentiation in vitro and new bone formation in vivo using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect.					
35151707	3	79	theme	CS/PCL	503:508	arg1	scaffolds					513:521	CS/PCL@AL scaffolds	503:521	CS/PCL@AL scaffolds	503:521	We demonstrated that CS/PCL@AL scaffolds significantly enhanced the osteogenic differentiation of ectomesenchymal stem cells (EMSCs) in vitro.					
35151707	5	80	theme	@	853:853	arg1	scaffold					857:864	the CS/PCL@AL scaffold	843:864	the CS/PCL@AL scaffold	843:864	This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1) the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2) the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP) signaling pathway.					
35151707	6	81	theme	EMSCs	1243:1247	arg1	differentiation					1224:1238	the osteogenic differentiation	1209:1238	the osteogenic differentiation of EMSCs	1209:1247	YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs.					
35151707	1	82	with	rats	248:251	arg1	defect					285:290	a critical-sized calvarial defect	258:290	a critical-sized calvarial defect	258:290	Here, we evaluated osteogenic differentiation in vitro and new bone formation in vivo using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect.					
35151707	3	83	theme	@	509:509	arg1	scaffolds					513:521	CS/PCL@AL scaffolds	503:521	CS/PCL@AL scaffolds	503:521	We demonstrated that CS/PCL@AL scaffolds significantly enhanced the osteogenic differentiation of ectomesenchymal stem cells (EMSCs) in vitro.					
35151707	1	84	theme	critical-sized	260:273	arg1	defect					285:290	a critical-sized calvarial defect	258:290	a critical-sized calvarial defect	258:290	Here, we evaluated osteogenic differentiation in vitro and new bone formation in vivo using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL) in rats with a critical-sized calvarial defect.					
36283439	9	0	theme	food	1200:1203	arg1	pathogens					1205:1213	tested food pathogens	1193:1213	tested food pathogens	1193:1213	The antibacterial activity of the amalgamated AgNPs against five bacterial pathogens studied was found to be highly active against tested food pathogens, except for Proteus vulgari.					
36283439	8	1	theme	group	1019:1023	arg1	changes					1025:1031	the functional group changes	1004:1031	the functional group changes	1004:1031	The Fourier transform infrared spectroscopic studies (FT-IR) disclose the functional group changes during the film preparation.					
36283439	6	2	contain	have	762:765	arg2	peak					779:782	an elevated peak	767:782	an elevated peak with a crystallinity of 37.5%	767:812	The X-ray diffraction (XRD) studies of the final film prepared have an elevated peak with a crystallinity of 37.5%.					
36283439	6	2	contain	have	762:765	arg1	studies					727:733	The X-ray diffraction (XRD) studies	699:733	The X-ray diffraction (XRD) studies of the final film prepared	699:760	The X-ray diffraction (XRD) studies of the final film prepared have an elevated peak with a crystallinity of 37.5%.					
36283439	5	3	theme	crystallite	654:664	arg1	size					666:669	The determined average crystallite size	631:669	The determined average crystallite size of AgNPs	631:678	The determined average crystallite size of AgNPs was seen at 20 nm.					
36283439	1	4	dep	film	235:238	arg1	based					240:244	based	240:244	based on microcrystalline cellulose (MCC)	240:280	In order to be used in food packaging, the study aims to develop a composite film based on microcrystalline cellulose (MCC) and coated with silver nanoparticles (AgNPs).					
36283439	1	4	dep	film	235:238	arg1	coated					286:291	coated	286:291	coated with silver nanoparticles (AgNPs)	286:325	In order to be used in food packaging, the study aims to develop a composite film based on microcrystalline cellulose (MCC) and coated with silver nanoparticles (AgNPs).					
36283439	6	5	theme	final	742:746	arg1	film					748:751	the final film	738:751	the final film prepared	738:760	The X-ray diffraction (XRD) studies of the final film prepared have an elevated peak with a crystallinity of 37.5%.					
36283439	5	6	theme	AgNPs	674:678	arg1	size					666:669	The determined average crystallite size	631:669	The determined average crystallite size of AgNPs	631:678	The determined average crystallite size of AgNPs was seen at 20 nm.					
36283439	10	7	theme	shelf	1329:1333	arg1	life					1335:1338	an increased shelf life	1316:1338	an increased shelf life for the vegetable	1316:1356	When coated over a vegetable, the produced nanocomposite film displayed an increased shelf life for the vegetable by limiting the decay impact caused by food pathogens.					
36283439	10	8	theme	produced	1278:1285	arg1	film					1301:1304	the produced nanocomposite film	1274:1304	the produced nanocomposite film	1274:1304	When coated over a vegetable, the produced nanocomposite film displayed an increased shelf life for the vegetable by limiting the decay impact caused by food pathogens.					
36283439	7	9	theme	AgNPs	869:873	arg1	SEM					857:859	SEM	857:859	SEM	857:859	The scanning electron microscopic images (SEM) of the AgNPs and the prepared samples, reveal their surface morphology.					
36283439	7	9	theme	AgNPs	869:873	arg1	samples					892:898	the prepared samples	879:898	the prepared samples	879:898	The scanning electron microscopic images (SEM) of the AgNPs and the prepared samples, reveal their surface morphology.					
36283439	7	9	theme	AgNPs	869:873	arg1	images					849:854	The scanning electron microscopic images	815:854	The scanning electron microscopic images (SEM) of the AgNPs	815:873	The scanning electron microscopic images (SEM) of the AgNPs and the prepared samples, reveal their surface morphology.					
36283439	0	10	theme	AgNP/MCC/starch/whey	95:114	arg1	film					89:92	impregnated nano composite film	62:92	silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications	41:155	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	0	10	theme	AgNP/MCC/starch/whey	95:114	arg1	protein					116:122	AgNP/MCC/starch/whey protein	95:122	AgNP/MCC/starch/whey protein	95:122	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	6	11	theme	%	812:812	arg1	crystallinity					791:803	a crystallinity	789:803	a crystallinity of 37.5%	789:812	The X-ray diffraction (XRD) studies of the final film prepared have an elevated peak with a crystallinity of 37.5%.					
36283439	9	12	theme	amalgamated	1096:1106	arg1	AgNPs					1108:1112	the amalgamated AgNPs	1092:1112	the amalgamated AgNPs	1092:1112	The antibacterial activity of the amalgamated AgNPs against five bacterial pathogens studied was found to be highly active against tested food pathogens, except for Proteus vulgari.					
36283439	10	13	theme	food	1397:1400	arg1	pathogens					1402:1410	food pathogens	1397:1410	food pathogens	1397:1410	When coated over a vegetable, the produced nanocomposite film displayed an increased shelf life for the vegetable by limiting the decay impact caused by food pathogens.					
36283439	1	14	theme	microcrystalline	249:264	arg1	MCC					277:279	MCC	277:279	MCC	277:279	In order to be used in food packaging, the study aims to develop a composite film based on microcrystalline cellulose (MCC) and coated with silver nanoparticles (AgNPs).					
36283439	1	14	theme	microcrystalline	249:264	arg1	cellulose					266:274	microcrystalline cellulose	249:274	microcrystalline cellulose (MCC)	249:280	In order to be used in food packaging, the study aims to develop a composite film based on microcrystalline cellulose (MCC) and coated with silver nanoparticles (AgNPs).					
36283439	9	15	theme	AgNPs	1108:1112	arg1	active					1178:1183	active	1178:1183	active	1178:1183	The antibacterial activity of the amalgamated AgNPs against five bacterial pathogens studied was found to be highly active against tested food pathogens, except for Proteus vulgari.					
36283439	9	15	theme	AgNPs	1108:1112	arg1	activity					1080:1087	The antibacterial activity	1062:1087	The antibacterial activity of the amalgamated AgNPs against five bacterial pathogens studied	1062:1153	The antibacterial activity of the amalgamated AgNPs against five bacterial pathogens studied was found to be highly active against tested food pathogens, except for Proteus vulgari.					
36283439	4	16	theme	reducing	615:622	arg1	agent					624:628	reducing agent	615:628	reducing agent	615:628	The AgNPs was synthesized by a green route employing Azadirachtaindica leaf extract as reducing agent.					
36283439	2	17	theme	cane	359:362	arg1	bagasse					364:370	sugar cane bagasse	353:370	sugar cane bagasse	353:370	The MCC was derived from sugar cane bagasse.					
36283439	10	18	theme	decay	1374:1378	arg1	impact					1380:1385	the decay impact	1370:1385	the decay impact caused by food pathogens	1370:1410	When coated over a vegetable, the produced nanocomposite film displayed an increased shelf life for the vegetable by limiting the decay impact caused by food pathogens.					
36283439	3	19	theme	poly-ethylene	394:406	arg1	glycol					408:413	poly-ethylene glycol 1500	394:418	poly-ethylene glycol 1500 (PEG-1500)	394:429	Protein, starch, and poly-ethylene glycol 1500 (PEG-1500) are employed to improve the tensile strength, flexibility, and durability of the packaging film.					
36283439	3	19	theme	poly-ethylene	394:406	arg1	PEG-1500					421:428	PEG-1500	421:428	PEG-1500	421:428	Protein, starch, and poly-ethylene glycol 1500 (PEG-1500) are employed to improve the tensile strength, flexibility, and durability of the packaging film.					
36283439	0	20	theme	indica	12:17	arg1	extract					24:30	Azadirachta indica leaf extract	0:30	Azadirachta indica leaf extract	0:30	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	8	21	theme	film	1044:1047	arg1	preparation					1049:1059	the film preparation	1040:1059	the film preparation	1040:1059	The Fourier transform infrared spectroscopic studies (FT-IR) disclose the functional group changes during the film preparation.					
36283439	4	22	theme	leaf	599:602	arg1	extract					604:610	Azadirachtaindica leaf extract	581:610	Azadirachtaindica leaf extract	581:610	The AgNPs was synthesized by a green route employing Azadirachtaindica leaf extract as reducing agent.					
36283439	1	23	theme	food	181:184	arg1	packaging					186:194	food packaging	181:194	food packaging	181:194	In order to be used in food packaging, the study aims to develop a composite film based on microcrystalline cellulose (MCC) and coated with silver nanoparticles (AgNPs).					
36283439	0	24	theme	Azadirachta	0:10	arg1	extract					24:30	Azadirachta indica leaf extract	0:30	Azadirachta indica leaf extract	0:30	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	8	25	theme	spectroscopic	965:977	arg1	FT-IR					988:992	FT-IR	988:992	FT-IR	988:992	The Fourier transform infrared spectroscopic studies (FT-IR) disclose the functional group changes during the film preparation.					
36283439	8	25	theme	spectroscopic	965:977	arg1	studies					979:985	spectroscopic studies	965:985	spectroscopic studies (FT-IR)	965:993	The Fourier transform infrared spectroscopic studies (FT-IR) disclose the functional group changes during the film preparation.					
36283439	7	26	theme	microscopic	837:847	arg1	SEM					857:859	SEM	857:859	SEM	857:859	The scanning electron microscopic images (SEM) of the AgNPs and the prepared samples, reveal their surface morphology.					
36283439	7	26	theme	microscopic	837:847	arg1	images					849:854	The scanning electron microscopic images	815:854	The scanning electron microscopic images (SEM) of the AgNPs	815:873	The scanning electron microscopic images (SEM) of the AgNPs and the prepared samples, reveal their surface morphology.					
36283439	3	27	theme	packaging	512:520	arg1	film					522:525	the packaging film	508:525	the packaging film	508:525	Protein, starch, and poly-ethylene glycol 1500 (PEG-1500) are employed to improve the tensile strength, flexibility, and durability of the packaging film.					
36283439	9	28	theme	bacterial	1127:1135	arg1	pathogens					1137:1145	five bacterial pathogens	1122:1145	five bacterial pathogens studied	1122:1153	The antibacterial activity of the amalgamated AgNPs against five bacterial pathogens studied was found to be highly active against tested food pathogens, except for Proteus vulgari.					
36283439	0	29	theme	packaging	134:142	arg1	applications					144:155	food packaging applications	129:155	food packaging applications	129:155	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	6	30	theme	diffraction	709:719	arg1	studies					727:733	The X-ray diffraction (XRD) studies	699:733	The X-ray diffraction (XRD) studies of the final film prepared	699:760	The X-ray diffraction (XRD) studies of the final film prepared have an elevated peak with a crystallinity of 37.5%.					
36283439	0	31	theme	leaf	19:22	arg1	extract					24:30	Azadirachta indica leaf extract	0:30	Azadirachta indica leaf extract	0:30	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	0	32	theme	food	129:132	arg1	applications					144:155	food packaging applications	129:155	food packaging applications	129:155	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	4	33	theme	green	559:563	arg1	route					565:569	a green route	557:569	a green route employing Azadirachtaindica leaf extract as reducing agent	557:628	The AgNPs was synthesized by a green route employing Azadirachtaindica leaf extract as reducing agent.					
36283439	0	34	theme	silver	41:46	arg1	nanoparticles					48:60	silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein)	41:123	silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications	41:155	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	11	35	theme	antimicrobial	1525:1537	arg1	material					1549:1556	an antimicrobial packaging material	1522:1556	an antimicrobial packaging material	1522:1556	According to the findings, the AgNPs-impregnated MCC/Starch/Whey protein has the potential to be employed as an antimicrobial packaging material.					
36283439	4	36	theme	Azadirachtaindica	581:597	arg1	extract					604:610	Azadirachtaindica leaf extract	581:610	Azadirachtaindica leaf extract	581:610	The AgNPs was synthesized by a green route employing Azadirachtaindica leaf extract as reducing agent.					
36283439	6	37	with	peak	779:782	arg1	crystallinity					791:803	a crystallinity	789:803	a crystallinity of 37.5%	789:812	The X-ray diffraction (XRD) studies of the final film prepared have an elevated peak with a crystallinity of 37.5%.					
36283439	10	38	theme	nanocomposite	1287:1299	arg1	film					1301:1304	the produced nanocomposite film	1274:1304	the produced nanocomposite film	1274:1304	When coated over a vegetable, the produced nanocomposite film displayed an increased shelf life for the vegetable by limiting the decay impact caused by food pathogens.					
36283439	11	39	theme	MCC/Starch/Whey	1462:1476	arg1	protein					1478:1484	the AgNPs-impregnated MCC/Starch/Whey protein	1440:1484	the AgNPs-impregnated MCC/Starch/Whey protein	1440:1484	According to the findings, the AgNPs-impregnated MCC/Starch/Whey protein has the potential to be employed as an antimicrobial packaging material.					
36283439	0	40	dep	nanoparticles	48:60	arg1	film					89:92	impregnated nano composite film	62:92	silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications	41:155	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	0	40	dep	nanoparticles	48:60	arg1	protein					116:122	AgNP/MCC/starch/whey protein	95:122	AgNP/MCC/starch/whey protein	95:122	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	6	41	theme	X-ray	703:707	arg1	XRD					722:724	XRD	722:724	XRD	722:724	The X-ray diffraction (XRD) studies of the final film prepared have an elevated peak with a crystallinity of 37.5%.					
36283439	6	41	theme	X-ray	703:707	arg1	diffraction					709:719	X-ray diffraction	703:719	The X-ray diffraction (XRD) studies of the final film prepared	699:760	The X-ray diffraction (XRD) studies of the final film prepared have an elevated peak with a crystallinity of 37.5%.					
36283439	0	42	theme	impregnated	62:72	arg1	film					89:92	impregnated nano composite film	62:92	silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications	41:155	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	0	42	theme	impregnated	62:72	arg1	protein					116:122	AgNP/MCC/starch/whey protein	95:122	AgNP/MCC/starch/whey protein	95:122	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	8	43	dep	Fourier	938:944	arg1	transform					946:954	transform	946:954	transform infrared spectroscopic studies (FT-IR)	946:993	The Fourier transform infrared spectroscopic studies (FT-IR) disclose the functional group changes during the film preparation.					
36283439	8	44	theme	functional	1008:1017	arg1	changes					1025:1031	the functional group changes	1004:1031	the functional group changes	1004:1031	The Fourier transform infrared spectroscopic studies (FT-IR) disclose the functional group changes during the film preparation.					
36283439	6	45	theme	elevated	770:777	arg1	peak					779:782	an elevated peak	767:782	an elevated peak with a crystallinity of 37.5%	767:812	The X-ray diffraction (XRD) studies of the final film prepared have an elevated peak with a crystallinity of 37.5%.					
36283439	3	46	theme	film	522:525	arg1	flexibility					477:487	flexibility	477:487	flexibility	477:487	Protein, starch, and poly-ethylene glycol 1500 (PEG-1500) are employed to improve the tensile strength, flexibility, and durability of the packaging film.					
36283439	3	46	theme	film	522:525	arg1	durability					494:503	durability	494:503	durability	494:503	Protein, starch, and poly-ethylene glycol 1500 (PEG-1500) are employed to improve the tensile strength, flexibility, and durability of the packaging film.					
36283439	3	46	theme	film	522:525	arg1	strength					467:474	the tensile strength	455:474	the tensile strength	455:474	Protein, starch, and poly-ethylene glycol 1500 (PEG-1500) are employed to improve the tensile strength, flexibility, and durability of the packaging film.					
36283439	7	47	theme	surface	914:920	arg1	morphology					922:931	their surface morphology	908:931	their surface morphology	908:931	The scanning electron microscopic images (SEM) of the AgNPs and the prepared samples, reveal their surface morphology.					
36283439	5	48	theme	average	646:652	arg1	size					666:669	The determined average crystallite size	631:669	The determined average crystallite size of AgNPs	631:678	The determined average crystallite size of AgNPs was seen at 20 nm.					
36283439	10	49	theme	increased	1319:1327	arg1	life					1335:1338	an increased shelf life	1316:1338	an increased shelf life for the vegetable	1316:1356	When coated over a vegetable, the produced nanocomposite film displayed an increased shelf life for the vegetable by limiting the decay impact caused by food pathogens.					
36283439	7	50	theme	scanning	819:826	arg1	SEM					857:859	SEM	857:859	SEM	857:859	The scanning electron microscopic images (SEM) of the AgNPs and the prepared samples, reveal their surface morphology.					
36283439	7	50	theme	scanning	819:826	arg1	images					849:854	The scanning electron microscopic images	815:854	The scanning electron microscopic images (SEM) of the AgNPs	815:873	The scanning electron microscopic images (SEM) of the AgNPs and the prepared samples, reveal their surface morphology.					
36283439	1	51	theme	silver	298:303	arg1	AgNPs					320:324	AgNPs	320:324	AgNPs	320:324	In order to be used in food packaging, the study aims to develop a composite film based on microcrystalline cellulose (MCC) and coated with silver nanoparticles (AgNPs).					
36283439	1	51	theme	silver	298:303	arg1	nanoparticles					305:317	silver nanoparticles	298:317	silver nanoparticles (AgNPs)	298:325	In order to be used in food packaging, the study aims to develop a composite film based on microcrystalline cellulose (MCC) and coated with silver nanoparticles (AgNPs).					
36283439	11	52	theme	AgNPs-impregnated	1444:1460	arg1	protein					1478:1484	the AgNPs-impregnated MCC/Starch/Whey protein	1440:1484	the AgNPs-impregnated MCC/Starch/Whey protein	1440:1484	According to the findings, the AgNPs-impregnated MCC/Starch/Whey protein has the potential to be employed as an antimicrobial packaging material.					
36283439	8	53	dep	transform	946:954	arg1	infrared					956:963	infrared	956:963	transform infrared spectroscopic studies (FT-IR)	946:993	The Fourier transform infrared spectroscopic studies (FT-IR) disclose the functional group changes during the film preparation.					
36283439	7	54	theme	electron	828:835	arg1	SEM					857:859	SEM	857:859	SEM	857:859	The scanning electron microscopic images (SEM) of the AgNPs and the prepared samples, reveal their surface morphology.					
36283439	7	54	theme	electron	828:835	arg1	images					849:854	The scanning electron microscopic images	815:854	The scanning electron microscopic images (SEM) of the AgNPs	815:873	The scanning electron microscopic images (SEM) of the AgNPs and the prepared samples, reveal their surface morphology.					
36283439	5	55	theme	determined	635:644	arg1	size					666:669	The determined average crystallite size	631:669	The determined average crystallite size of AgNPs	631:678	The determined average crystallite size of AgNPs was seen at 20 nm.					
36283439	2	56	attach	derived	340:346	arg2	MCC					332:334	The MCC	328:334	The MCC	328:334	The MCC was derived from sugar cane bagasse.					
36283439	2	56	attach	derived	340:346	arg1	bagasse					364:370	sugar cane bagasse	353:370	sugar cane bagasse	353:370	The MCC was derived from sugar cane bagasse.					
36283439	7	57	theme	prepared	883:890	arg1	samples					892:898	the prepared samples	879:898	the prepared samples	879:898	The scanning electron microscopic images (SEM) of the AgNPs and the prepared samples, reveal their surface morphology.					
36283439	0	58	theme	composite	79:87	arg1	film					89:92	impregnated nano composite film	62:92	silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications	41:155	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	0	58	theme	composite	79:87	arg1	protein					116:122	AgNP/MCC/starch/whey protein	95:122	AgNP/MCC/starch/whey protein	95:122	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	6	59	theme	film	748:751	arg1	studies					727:733	The X-ray diffraction (XRD) studies	699:733	The X-ray diffraction (XRD) studies of the final film prepared	699:760	The X-ray diffraction (XRD) studies of the final film prepared have an elevated peak with a crystallinity of 37.5%.					
36283439	11	60	theme	packaging	1539:1547	arg1	material					1549:1556	an antimicrobial packaging material	1522:1556	an antimicrobial packaging material	1522:1556	According to the findings, the AgNPs-impregnated MCC/Starch/Whey protein has the potential to be employed as an antimicrobial packaging material.					
36283439	3	61	theme	tensile	459:465	arg1	strength					467:474	the tensile strength	455:474	the tensile strength	455:474	Protein, starch, and poly-ethylene glycol 1500 (PEG-1500) are employed to improve the tensile strength, flexibility, and durability of the packaging film.					
36283439	2	62	theme	sugar	353:357	arg1	bagasse					364:370	sugar cane bagasse	353:370	sugar cane bagasse	353:370	The MCC was derived from sugar cane bagasse.					
36283439	0	63	theme	nano	74:77	arg1	film					89:92	impregnated nano composite film	62:92	silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications	41:155	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	0	63	theme	nano	74:77	arg1	protein					116:122	AgNP/MCC/starch/whey protein	95:122	AgNP/MCC/starch/whey protein	95:122	Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications.					
36283439	9	64	theme	tested	1193:1198	arg1	pathogens					1205:1213	tested food pathogens	1193:1213	tested food pathogens	1193:1213	The antibacterial activity of the amalgamated AgNPs against five bacterial pathogens studied was found to be highly active against tested food pathogens, except for Proteus vulgari.					
36283439	11	65	contain	has	1486:1488	arg1	protein					1478:1484	the AgNPs-impregnated MCC/Starch/Whey protein	1440:1484	the AgNPs-impregnated MCC/Starch/Whey protein	1440:1484	According to the findings, the AgNPs-impregnated MCC/Starch/Whey protein has the potential to be employed as an antimicrobial packaging material.					
36283439	11	65	contain	has	1486:1488	arg2	potential					1494:1502	the potential to be employed as an antimicrobial packaging material	1490:1556	the potential to be employed as an antimicrobial packaging material	1490:1556	According to the findings, the AgNPs-impregnated MCC/Starch/Whey protein has the potential to be employed as an antimicrobial packaging material.					
36283439	9	66	theme	antibacterial	1066:1078	arg1	active					1178:1183	active	1178:1183	active	1178:1183	The antibacterial activity of the amalgamated AgNPs against five bacterial pathogens studied was found to be highly active against tested food pathogens, except for Proteus vulgari.					
36283439	9	66	theme	antibacterial	1066:1078	arg1	activity					1080:1087	The antibacterial activity	1062:1087	The antibacterial activity of the amalgamated AgNPs against five bacterial pathogens studied	1062:1153	The antibacterial activity of the amalgamated AgNPs against five bacterial pathogens studied was found to be highly active against tested food pathogens, except for Proteus vulgari.					
36283439	1	67	theme	composite	225:233	arg1	film					235:238	a composite film	223:238	a composite film based on microcrystalline cellulose (MCC) and coated with silver nanoparticles (AgNPs)	223:325	In order to be used in food packaging, the study aims to develop a composite film based on microcrystalline cellulose (MCC) and coated with silver nanoparticles (AgNPs).					
35432720	7	0	theme	further	1592:1598	arg1	studies					1609:1615	further clinical studies	1592:1615	further clinical studies	1592:1615	However, this study was only performed using animal models, and the lipid synthesis and metabolism mechanism are complicated; hence, further clinical studies are needed.					
35432720	2	1	theme	mice	537:540	arg1	indexes					526:532	the body weight, epididymal fat index, blood lipid level, and liver function indexes	449:532	the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice	449:540	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	1	2	theme	AX	247:248	arg1	HFY06					267:271	AX and L. fermentum HFY06	247:271	AX and L. fermentum HFY06	247:271	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	6	3	contain	have	1404:1407	arg1	AX					1378:1379	AX	1378:1379	AX	1378:1379	From the above results, it can be concluded that AX and L. fermentum HFY06 have a synergistic effect in improving hyperlipidemia.					
35432720	6	3	contain	have	1404:1407	arg2	effect					1423:1428	a synergistic effect	1409:1428	a synergistic effect	1409:1428	From the above results, it can be concluded that AX and L. fermentum HFY06 have a synergistic effect in improving hyperlipidemia.					
35432720	6	3	contain	have	1404:1407	arg1	fermentum					1388:1396	fermentum	1388:1396	fermentum	1388:1396	From the above results, it can be concluded that AX and L. fermentum HFY06 have a synergistic effect in improving hyperlipidemia.					
35432720	5	4	theme	mice	1213:1216	arg1	intestine					1200:1208	the small intestine	1190:1208	the small intestine of mice	1190:1216	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	6	5	theme	above	1338:1342	arg1	results					1344:1350	the above results	1334:1350	the above results	1334:1350	From the above results, it can be concluded that AX and L. fermentum HFY06 have a synergistic effect in improving hyperlipidemia.					
35432720	2	6	theme	function	517:524	arg1	indexes					526:532	the body weight, epididymal fat index, blood lipid level, and liver function indexes	449:532	the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice	449:540	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	4	7	theme	PPAR-α	976:981	arg1	expressions					954:964	the mRNA expressions	945:964	the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL	945:998	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	3	8	theme	mice	665:668	arg1	serum					656:660	the serum	652:660	the serum of mice	652:668	In addition, the synbiotics comprising AX and L. fermentum HFY06 increased the CAT activity in the serum of mice on a high-fat diet, reduced NO and MDA levels, and improved the body's oxidative stress.					
35432720	0	9	theme	High-Fat	115:122	arg1	Diet					124:127	High-Fat Diet	115:127	High-Fat Diet	115:127	Effect of Lactobacillus fermentum HFY06 Combined with Arabinoxylan on Reducing Lipid Accumulation in Mice Fed with High-Fat Diet.					
35432720	2	10	theme	lipid	494:498	arg1	level					500:504	blood lipid level	488:504	blood lipid level	488:504	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	4	11	theme	CYP7A1	984:989	arg1	expressions					954:964	the mRNA expressions	945:964	the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL	945:998	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	1	12	from	effect	237:242	arg1	prevention					280:289	the prevention	276:289	the prevention of hyperlipidemia and its potential regulatory mechanism	276:346	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	1	13	from	hyperlipidemia	185:198	arg1	mice					203:206	mice	203:206	mice	203:206	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	4	14	theme	biology	793:799	arg1	perspective					768:778	the perspective	764:778	the perspective of molecular biology	764:799	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	3	15	from	activity	640:647	arg1	serum					656:660	the serum	652:660	the serum of mice	652:668	In addition, the synbiotics comprising AX and L. fermentum HFY06 increased the CAT activity in the serum of mice on a high-fat diet, reduced NO and MDA levels, and improved the body's oxidative stress.					
35432720	3	15	from	activity	640:647	arg1	diet					684:687	a high-fat diet	673:687	a high-fat diet	673:687	In addition, the synbiotics comprising AX and L. fermentum HFY06 increased the CAT activity in the serum of mice on a high-fat diet, reduced NO and MDA levels, and improved the body's oxidative stress.					
35432720	2	16	theme	liver	511:515	arg1	function					517:524	liver function	511:524	liver function	511:524	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	1	17	used	used	170:173	arg2	diet					161:164	a high-fat diet	150:164	a high-fat diet	150:164	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	5	18	theme	AX	1111:1112	arg1	effect					1101:1106	the synergistic effect	1085:1106	the synergistic effect of AX and HFY06	1085:1122	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	4	19	theme	synergistic	845:855	arg1	intervention					857:868	AX and L. fermentum HFY06 synergistic intervention	819:868	AX and L. fermentum HFY06 synergistic intervention	819:868	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	5	20	theme	HFY06	1118:1122	arg1	effect					1101:1106	the synergistic effect	1085:1106	the synergistic effect of AX and HFY06	1085:1122	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	1	21	theme	hyperlipidemia	294:307	arg1	prevention					280:289	the prevention	276:289	the prevention of hyperlipidemia and its potential regulatory mechanism	276:346	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	2	22	theme	AX	397:398	arg1	intervention					435:446	the AX and L. fermentum HFY06 synergistic intervention	393:446	the AX and L. fermentum HFY06 synergistic intervention	393:446	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	7	23	theme	animal	1504:1509	arg1	models					1511:1516	animal models	1504:1516	animal models	1504:1516	However, this study was only performed using animal models, and the lipid synthesis and metabolism mechanism are complicated; hence, further clinical studies are needed.					
35432720	2	24	theme	body	453:456	arg1	weight					458:463	body weight	453:463	body weight	453:463	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	4	25	theme	mRNA	1024:1027	arg1	expressions					1029:1039	the mRNA expressions	1020:1039	the mRNA expressions of ACC, C/EBPα, and LPL	1020:1063	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	0	26	theme	Lipid	79:83	arg1	Accumulation					85:96	Lipid Accumulation	79:96	Lipid Accumulation in Mice Fed with High-Fat Diet	79:127	Effect of Lactobacillus fermentum HFY06 Combined with Arabinoxylan on Reducing Lipid Accumulation in Mice Fed with High-Fat Diet.					
35432720	1	27	dep	L.	254:255	arg1	fermentum					257:265	fermentum	257:265	fermentum	257:265	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	1	28	theme	regulatory	327:336	arg1	mechanism					338:346	its potential regulatory mechanism	313:346	its potential regulatory mechanism	313:346	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	2	29	theme	fat	477:479	arg1	index					481:485	epididymal fat index	466:485	epididymal fat index	466:485	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	5	30	theme	barrier	1260:1266	arg1	strength					1233:1240	the strength	1229:1240	the strength of the intestinal barrier	1229:1266	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	5	31	theme	ZO-1	1157:1160	arg1	expressions					1142:1152	the mRNA expressions	1133:1152	the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice	1133:1216	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	2	32	theme	study	369:373	arg1	results					353:359	The results	349:359	The results of this study	349:373	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	4	33	theme	body	909:912	arg1	metabolism					920:929	body lipid metabolism	909:929	body lipid metabolism	909:929	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	5	34	theme	occludin	1163:1170	arg1	expressions					1142:1152	the mRNA expressions	1133:1152	the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice	1133:1216	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	2	35	dep	L.	404:405	arg1	fermentum					407:415	fermentum	407:415	fermentum	407:415	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	4	36	theme	LPL	1061:1063	arg1	expressions					1029:1039	the mRNA expressions	1020:1039	the mRNA expressions of ACC, C/EBPα, and LPL	1020:1063	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	0	37	theme	Lactobacillus	10:22	arg1	HFY06					34:38	Lactobacillus fermentum HFY06	10:38	Lactobacillus fermentum HFY06 Combined with Arabinoxylan	10:65	Effect of Lactobacillus fermentum HFY06 Combined with Arabinoxylan on Reducing Lipid Accumulation in Mice Fed with High-Fat Diet.					
35432720	4	38	theme	AMPK	884:887	arg1	pathway					889:895	the AMPK pathway	880:895	the AMPK pathway	880:895	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	0	39	theme	HFY06	34:38	arg1	Effect					0:5	Effect	0:5	Effect of Lactobacillus fermentum HFY06 Combined with Arabinoxylan on Reducing Lipid Accumulation in Mice Fed with High-Fat Diet.	0:128	Effect of Lactobacillus fermentum HFY06 Combined with Arabinoxylan on Reducing Lipid Accumulation in Mice Fed with High-Fat Diet.					
35432720	2	40	theme	synergistic	423:433	arg1	intervention					435:446	the AX and L. fermentum HFY06 synergistic intervention	393:446	the AX and L. fermentum HFY06 synergistic intervention	393:446	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	5	41	theme	intestinal	1306:1315	arg1	microbiota					1317:1326	the intestinal microbiota	1302:1326	the intestinal microbiota	1302:1326	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	2	42	theme	weight	458:463	arg1	indexes					526:532	the body weight, epididymal fat index, blood lipid level, and liver function indexes	449:532	the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice	449:540	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	5	43	theme	small	1194:1198	arg1	intestine					1200:1208	the small intestine	1190:1208	the small intestine of mice	1190:1216	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	0	44	dep	Lactobacillus	10:22	arg1	fermentum					24:32	fermentum	24:32	fermentum	24:32	Effect of Lactobacillus fermentum HFY06 Combined with Arabinoxylan on Reducing Lipid Accumulation in Mice Fed with High-Fat Diet.					
35432720	7	45	theme	clinical	1600:1607	arg1	studies					1609:1615	further clinical studies	1592:1615	further clinical studies	1592:1615	However, this study was only performed using animal models, and the lipid synthesis and metabolism mechanism are complicated; hence, further clinical studies are needed.					
35432720	5	46	theme	other	1073:1077	arg1	hand					1079:1082	the other hand	1069:1082	the other hand	1069:1082	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	3	47	theme	MDA	705:707	arg1	levels					709:714	NO and MDA levels	698:714	levels	709:714	In addition, the synbiotics comprising AX and L. fermentum HFY06 increased the CAT activity in the serum of mice on a high-fat diet, reduced NO and MDA levels, and improved the body's oxidative stress.					
35432720	7	48	dep	synthesis	1533:1541	arg1	mechanism					1558:1566	mechanism	1558:1566	mechanism	1558:1566	However, this study was only performed using animal models, and the lipid synthesis and metabolism mechanism are complicated; hence, further clinical studies are needed.					
35432720	1	49	theme	synergistic	225:235	arg1	effect					237:242	the synergistic effect	221:242	the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism	221:346	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	4	50	theme	mRNA	949:952	arg1	expressions					954:964	the mRNA expressions	945:964	the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL	945:998	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	4	51	theme	HSL	996:998	arg1	expressions					954:964	the mRNA expressions	945:964	the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL	945:998	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	5	52	theme	intestinal	1249:1258	arg1	barrier					1260:1266	the intestinal barrier	1245:1266	the intestinal barrier	1245:1266	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	1	53	theme	high-fat	152:159	arg1	diet					161:164	a high-fat diet	150:164	a high-fat diet	150:164	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	3	54	theme	high-fat	675:682	arg1	diet					684:687	a high-fat diet	673:687	a high-fat diet	673:687	In addition, the synbiotics comprising AX and L. fermentum HFY06 increased the CAT activity in the serum of mice on a high-fat diet, reduced NO and MDA levels, and improved the body's oxidative stress.					
35432720	2	55	theme	level	500:504	arg1	indexes					526:532	the body weight, epididymal fat index, blood lipid level, and liver function indexes	449:532	the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice	449:540	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	0	56	from	Accumulation	85:96	arg1	Mice					101:104	Mice	101:104	Mice Fed with High-Fat Diet	101:127	Effect of Lactobacillus fermentum HFY06 Combined with Arabinoxylan on Reducing Lipid Accumulation in Mice Fed with High-Fat Diet.					
35432720	2	57	theme	blood	488:492	arg1	level					500:504	blood lipid level	488:504	blood lipid level	488:504	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	5	58	from	expressions	1142:1152	arg1	intestine					1200:1208	the small intestine	1190:1208	the small intestine of mice	1190:1216	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	4	59	theme	ACC	1044:1046	arg1	expressions					1029:1039	the mRNA expressions	1020:1039	the mRNA expressions of ACC, C/EBPα, and LPL	1020:1063	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	3	60	theme	NO	698:699	arg1	levels					709:714	NO and MDA levels	698:714	levels	709:714	In addition, the synbiotics comprising AX and L. fermentum HFY06 increased the CAT activity in the serum of mice on a high-fat diet, reduced NO and MDA levels, and improved the body's oxidative stress.					
35432720	4	61	theme	molecular	783:791	arg1	biology					793:799	molecular biology	783:799	molecular biology	783:799	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	4	62	theme	C/EBPα	1049:1054	arg1	expressions					1029:1039	the mRNA expressions	1020:1039	the mRNA expressions of ACC, C/EBPα, and LPL	1020:1063	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	7	63	theme	lipid	1527:1531	arg1	synthesis					1533:1541	the lipid synthesis	1523:1541	the lipid synthesis	1523:1541	However, this study was only performed using animal models, and the lipid synthesis and metabolism mechanism are complicated; hence, further clinical studies are needed.					
35432720	3	64	theme	oxidative	741:749	arg1	stress					751:756	the body's oxidative stress	730:756	the body's oxidative stress	730:756	In addition, the synbiotics comprising AX and L. fermentum HFY06 increased the CAT activity in the serum of mice on a high-fat diet, reduced NO and MDA levels, and improved the body's oxidative stress.					
35432720	4	65	theme	HFY06	839:843	arg1	intervention					857:868	AX and L. fermentum HFY06 synergistic intervention	819:868	AX and L. fermentum HFY06 synergistic intervention	819:868	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	4	66	theme	AX	819:820	arg1	intervention					857:868	AX and L. fermentum HFY06 synergistic intervention	819:868	AX and L. fermentum HFY06 synergistic intervention	819:868	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	4	67	dep	L.	826:827	arg1	fermentum					829:837	fermentum	829:837	fermentum	829:837	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	5	68	theme	synergistic	1089:1099	arg1	effect					1101:1106	the synergistic effect	1085:1106	the synergistic effect of AX and HFY06	1085:1122	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	1	69	theme	potential	317:325	arg1	mechanism					338:346	its potential regulatory mechanism	313:346	its potential regulatory mechanism	313:346	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	2	70	theme	index	481:485	arg1	indexes					526:532	the body weight, epididymal fat index, blood lipid level, and liver function indexes	449:532	the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice	449:540	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	1	71	theme	mechanism	338:346	arg1	prevention					280:289	the prevention	276:289	the prevention of hyperlipidemia and its potential regulatory mechanism	276:346	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	6	72	theme	synergistic	1411:1421	arg1	effect					1423:1428	a synergistic effect	1409:1428	a synergistic effect	1409:1428	From the above results, it can be concluded that AX and L. fermentum HFY06 have a synergistic effect in improving hyperlipidemia.					
35432720	2	73	theme	epididymal	466:475	arg1	index					481:485	epididymal fat index	466:485	epididymal fat index	466:485	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	2	74	theme	L.	404:405	arg1	intervention					435:446	the AX and L. fermentum HFY06 synergistic intervention	393:446	the AX and L. fermentum HFY06 synergistic intervention	393:446	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	1	75	theme	L.	254:255	arg1	HFY06					267:271	AX and L. fermentum HFY06	247:271	AX and L. fermentum HFY06	247:271	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	4	76	theme	lipid	914:918	arg1	metabolism					920:929	body lipid metabolism	909:929	body lipid metabolism	909:929	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	5	77	theme	mRNA	1137:1140	arg1	expressions					1142:1152	the mRNA expressions	1133:1152	the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice	1133:1216	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	1	78	theme	HFY06	267:271	arg1	effect					237:242	the synergistic effect	221:242	the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism	221:346	In this experiment, a high-fat diet was used to induce hyperlipidemia in mice to determine the synergistic effect of AX and L. fermentum HFY06 on the prevention of hyperlipidemia and its potential regulatory mechanism.					
35432720	4	79	theme	L.	826:827	arg1	intervention					857:868	AX and L. fermentum HFY06 synergistic intervention	819:868	AX and L. fermentum HFY06 synergistic intervention	819:868	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	2	80	theme	HFY06	417:421	arg1	intervention					435:446	the AX and L. fermentum HFY06 synergistic intervention	393:446	the AX and L. fermentum HFY06 synergistic intervention	393:446	The results of this study showed that after the AX and L. fermentum HFY06 synergistic intervention, the body weight, epididymal fat index, blood lipid level, and liver function indexes of mice were improved.					
35432720	5	81	theme	microbiota	1317:1326	arg1	composition					1287:1297	the composition	1283:1297	the composition of the intestinal microbiota	1283:1326	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35432720	7	82	dep	complicated	1572:1582	arg1	needed					1621:1626	needed	1621:1626	are needed	1617:1626	However, this study was only performed using animal models, and the lipid synthesis and metabolism mechanism are complicated; hence, further clinical studies are needed.					
35432720	4	83	theme	CPT-1	969:973	arg1	expressions					954:964	the mRNA expressions	945:964	the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL	945:998	From the perspective of molecular biology, on the one hand, AX and L. fermentum HFY06 synergistic intervention activated the AMPK pathway to regulate body lipid metabolism; up-regulated the mRNA expressions of CPT-1, PPAR-α, CYP7A1, and HSL; and down-regulated the mRNA expressions of ACC, C/EBPα, and LPL.					
35432720	3	84	theme	CAT	636:638	arg1	activity					640:647	the CAT activity	632:647	the CAT activity in the serum of mice on a high-fat diet	632:687	In addition, the synbiotics comprising AX and L. fermentum HFY06 increased the CAT activity in the serum of mice on a high-fat diet, reduced NO and MDA levels, and improved the body's oxidative stress.					
35432720	5	85	theme	claudin-1	1177:1185	arg1	expressions					1142:1152	the mRNA expressions	1133:1152	the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice	1133:1216	On the other hand, the synergistic effect of AX and HFY06 enhanced the mRNA expressions of ZO-1, occludin, and claudin-1 in the small intestine of mice, increased the strength of the intestinal barrier, and optimized the composition of the intestinal microbiota.					
35242132	9	0	theme	higher	1605:1610	arg1	release					1616:1622	significantly higher NET release	1591:1622	significantly higher NET release at 48 h compared to untreated cells	1591:1658	Similarly, incubation with G-CSF resulted in significantly higher NET release at 48 h compared to untreated cells.					
35242132	4	1	theme	surviving	486:494	arg1	neutrophils					496:506	These long-term surviving neutrophils	470:506	These long-term surviving neutrophils	470:506	These long-term surviving neutrophils are reported to maintain phagocytic activity and cytokine release; however, little is known regarding their capability to release NETs.					
35242132	6	2	theme	cell-free	1111:1119	arg1	DNA					1121:1123	cell-free DNA	1111:1123	cell-free DNA (cfDNA)	1111:1131	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	6	2	theme	cell-free	1111:1119	arg1	cfDNA					1126:1130	cfDNA	1126:1130	cfDNA	1126:1130	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	7	3	with	stimulation	1238:1248	arg1	PMA					1255:1257	PMA	1255:1257	PMA	1255:1257	RESULTS Untreated neutrophils could form NETs after stimulation with PMA for up to 24 h. Incubation with LPS extended their ability to form NETs for up to 48 h.					
35242132	13	4	theme	new	2112:2114	arg1	information					2116:2126	new information	2112:2126	new information for the use of neutrophils	2112:2153	These results provide new information for the use of neutrophils in long-term experiments for NET formation and provide novel insights for neutrophil behaviour under inflammatory conditions.					
35242132	2	5	theme	short	289:293	arg1	lifetime					295:302	their short lifetime	283:302	their short lifetime of only a few hours	283:322	Research on neutrophil granulocytes is limited because of their short lifetime of only a few hours.					
35242132	3	6	theme	bacterial	417:425	arg1	products					427:434	bacterial products	417:434	bacterial products	417:434	Several attempts have been made to prolong the half-life of neutrophils using cytokines and bacterial products and have shown promising results.					
35242132	0	7	theme	NET	0:2	arg1	Release					4:10	NET Release	0:10	NET Release of Long-Term Surviving Neutrophils	0:45	NET Release of Long-Term Surviving Neutrophils.					
35242132	5	8	theme	flow	867:870	arg1	cytometry					872:880	flow cytometry	867:880	flow cytometry	867:880	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	6	9	theme	12-myristate	980:991	arg1	PMA					1005:1007	PMA	1005:1007	PMA	1005:1007	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	6	9	theme	12-myristate	980:991	arg1	13-acetate					993:1002	phorbol 12-myristate 13-acetate	972:1002	phorbol 12-myristate 13-acetate (PMA)	972:1008	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	6	10	with	stimulation	955:965	arg1	PMA					1005:1007	PMA	1005:1007	PMA	1005:1007	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	6	10	with	stimulation	955:965	arg1	13-acetate					993:1002	phorbol 12-myristate 13-acetate	972:1002	phorbol 12-myristate 13-acetate (PMA)	972:1008	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	12	11	theme	NET	2059:2061	arg1	formation					2063:2071	NET formation	2059:2071	NET formation	2059:2071	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	5	12	theme	various	719:725	arg1	conditions					735:744	various culture conditions	719:744	various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α)	719:862	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	5	13	theme	necrosis	834:841	arg1	TNF-α					857:861	TNF-α	857:861	TNF-α	857:861	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	5	13	theme	necrosis	834:841	arg1	alpha					850:854	tumour necrosis factor alpha	827:854	tumour necrosis factor alpha (TNF-α)	827:862	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	12	14	from	differences	2044:2054	arg1	formation					2063:2071	NET formation	2059:2071	NET formation	2059:2071	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	12	14	from	differences	2044:2054	arg1	composition					2077:2087	composition	2077:2087	composition	2077:2087	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	5	15	theme	survival	695:702	arg1	prolongation					668:679	the prolongation	664:679	the prolongation of neutrophil survival	664:702	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	6	16	theme	NET	931:933	arg1	formation					935:943	NET formation	931:943	NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO)	931:1183	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	1	17	theme	immunity-are	130:141	arg1	swords					113:118	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords	48:118	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are	48:141	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	13	18	theme	inflammatory	2256:2267	arg1	conditions					2269:2278	inflammatory conditions	2256:2278	inflammatory conditions	2256:2278	These results provide new information for the use of neutrophils in long-term experiments for NET formation and provide novel insights for neutrophil behaviour under inflammatory conditions.					
35242132	12	19	theme	cultured	1996:2003	arg1	neutrophils					2005:2015	cultured neutrophils	1996:2015	cultured neutrophils	1996:2015	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	5	20	theme	viability	888:896	arg1	assay					898:902	a viability assay	886:902	a viability assay	886:902	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	6	21	theme	immunofluorescence	1013:1030	arg1	staining					1032:1039	immunofluorescence staining	1013:1039	immunofluorescence staining	1013:1039	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	13	22	theme	neutrophil	2229:2238	arg1	behaviour					2240:2248	neutrophil behaviour	2229:2248	neutrophil behaviour	2229:2248	These results provide new information for the use of neutrophils in long-term experiments for NET formation and provide novel insights for neutrophil behaviour under inflammatory conditions.					
35242132	5	23	theme	granulocyte	752:762	arg1	G-CSF					791:795	G-CSF	791:795	G-CSF	791:795	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	5	23	theme	granulocyte	752:762	arg1	factor					783:788	granulocyte colony-stimulating factor	752:788	granulocyte colony-stimulating factor (G-CSF)	752:796	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	1	24	theme	extracellular	70:82	arg1	swords					113:118	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords	48:118	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are	48:141	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	2	25	from	Research	225:232	arg1	granulocytes					248:259	neutrophil granulocytes	237:259	neutrophil granulocytes	237:259	Research on neutrophil granulocytes is limited because of their short lifetime of only a few hours.					
35242132	1	26	dep	extracellular	70:82	arg1	traps					84:88	traps	84:88	traps	84:88	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	7	27	dep	24 h.	1269:1273	arg1	to					1266:1267	to	1266:1267	to	1266:1267	RESULTS Untreated neutrophils could form NETs after stimulation with PMA for up to 24 h. Incubation with LPS extended their ability to form NETs for up to 48 h.					
35242132	6	28	theme	fluorometric	1087:1098	arg1	myeloperoxidase					1163:1177	myeloperoxidase	1163:1177	myeloperoxidase (MPO)	1163:1183	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	6	28	theme	fluorometric	1087:1098	arg1	elastase					1145:1152	neutrophil elastase	1134:1152	neutrophil elastase (NE)	1134:1157	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	6	28	theme	fluorometric	1087:1098	arg1	assays					1100:1105	fluorometric assays	1087:1105	fluorometric assays for cell-free DNA (cfDNA)	1087:1131	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	8	29	theme	different	1489:1497	arg1	activity					1509:1516	no significantly different enzymatic activity	1472:1516	no significantly different enzymatic activity of NE and MPO	1472:1530	At 48 h, NET release of neutrophils cultured with LPS was significantly higher compared to that of untreated cells; however, no significantly different enzymatic activity of NE and MPO was observed.					
35242132	11	30	theme	untreated	1843:1851	arg1	cells					1853:1857	untreated cells	1843:1857	untreated cells	1843:1857	Lastly, incubation with TNF-α had no influence on NET release compared to untreated cells although survival counts were altered by TNF-α.					
35242132	5	31	theme	tumour	827:832	arg1	TNF-α					857:861	TNF-α	857:861	TNF-α	857:861	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	5	31	theme	tumour	827:832	arg1	alpha					850:854	tumour necrosis factor alpha	827:854	tumour necrosis factor alpha (TNF-α)	827:862	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	13	32	theme	NET	2184:2186	arg1	formation					2188:2196	NET formation	2184:2196	NET formation	2184:2196	These results provide new information for the use of neutrophils in long-term experiments for NET formation and provide novel insights for neutrophil behaviour under inflammatory conditions.					
35242132	5	33	theme	factor	843:848	arg1	TNF-α					857:861	TNF-α	857:861	TNF-α	857:861	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	5	33	theme	factor	843:848	arg1	alpha					850:854	tumour necrosis factor alpha	827:854	tumour necrosis factor alpha (TNF-α)	827:862	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	1	34	theme	double-edged	100:111	arg1	swords					113:118	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords	48:118	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are	48:141	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	5	35	dep	METHODS	644:650	arg1	analysed					655:662	analysed	655:662	analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay	655:902	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	8	36	theme	NE	1521:1522	arg1	activity					1509:1516	no significantly different enzymatic activity	1472:1516	no significantly different enzymatic activity of NE and MPO	1472:1530	At 48 h, NET release of neutrophils cultured with LPS was significantly higher compared to that of untreated cells; however, no significantly different enzymatic activity of NE and MPO was observed.					
35242132	3	37	dep	Several	325:331	arg1	attempts					333:340	attempts	333:340	attempts	333:340	Several attempts have been made to prolong the half-life of neutrophils using cytokines and bacterial products and have shown promising results.					
35242132	8	38	theme	NET	1356:1358	arg1	release					1360:1366	NET release	1356:1366	NET release of neutrophils cultured with LPS	1356:1399	At 48 h, NET release of neutrophils cultured with LPS was significantly higher compared to that of untreated cells; however, no significantly different enzymatic activity of NE and MPO was observed.					
35242132	0	39	theme	Surviving	25:33	arg1	Neutrophils					35:45	Long-Term Surviving Neutrophils	15:45	Long-Term Surviving Neutrophils	15:45	NET Release of Long-Term Surviving Neutrophils.					
35242132	3	40	theme	neutrophils	385:395	arg1	half-life					372:380	the half-life	368:380	the half-life of neutrophils	368:395	Several attempts have been made to prolong the half-life of neutrophils using cytokines and bacterial products and have shown promising results.					
35242132	8	41	theme	MPO	1528:1530	arg1	activity					1509:1516	no significantly different enzymatic activity	1472:1516	no significantly different enzymatic activity of NE and MPO	1472:1530	At 48 h, NET release of neutrophils cultured with LPS was significantly higher compared to that of untreated cells; however, no significantly different enzymatic activity of NE and MPO was observed.					
35242132	11	42	contain	had	1799:1801	arg1	incubation					1777:1786	incubation	1777:1786	incubation with TNF-α	1777:1797	Lastly, incubation with TNF-α had no influence on NET release compared to untreated cells although survival counts were altered by TNF-α.					
35242132	11	42	contain	had	1799:1801	arg2	influence					1806:1814	no influence	1803:1814	no influence on NET release	1803:1829	Lastly, incubation with TNF-α had no influence on NET release compared to untreated cells although survival counts were altered by TNF-α.					
35242132	7	43	dep	RESULTS	1186:1192	arg1	form					1222:1225	form	1222:1225	could form NETs after stimulation with PMA for up to 24 h. Incubation with LPS extended their ability to form NETs for up to 48 h	1216:1344	RESULTS Untreated neutrophils could form NETs after stimulation with PMA for up to 24 h. Incubation with LPS extended their ability to form NETs for up to 48 h.					
35242132	10	44	theme	enzymatic	1707:1715	arg1	activity					1717:1724	significantly higher enzymatic activity	1686:1724	significantly higher enzymatic activity of NE and MPO	1686:1738	Furthermore, NETs showed significantly higher enzymatic activity of NE and MPO after incubation with G-CSF.					
35242132	10	45	theme	higher	1700:1705	arg1	activity					1717:1724	significantly higher enzymatic activity	1686:1724	significantly higher enzymatic activity of NE and MPO	1686:1738	Furthermore, NETs showed significantly higher enzymatic activity of NE and MPO after incubation with G-CSF.					
35242132	1	46	theme	BACKGROUND	48:57	arg1	swords					113:118	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords	48:118	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are	48:141	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	4	47	theme	phagocytic	533:542	arg1	activity					544:551	phagocytic activity	533:551	phagocytic activity	533:551	These long-term surviving neutrophils are reported to maintain phagocytic activity and cytokine release; however, little is known regarding their capability to release NETs.					
35242132	11	48	theme	NET	1819:1821	arg1	release					1823:1829	NET release	1819:1829	NET release	1819:1829	Lastly, incubation with TNF-α had no influence on NET release compared to untreated cells although survival counts were altered by TNF-α.					
35242132	12	49	theme	prolonged	1974:1982	arg1	survival					1984:1991	prolonged survival	1974:1991	prolonged survival of cultured neutrophils	1974:2015	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	9	50	theme	NET	1612:1614	arg1	release					1616:1622	significantly higher NET release	1591:1622	significantly higher NET release at 48 h compared to untreated cells	1591:1658	Similarly, incubation with G-CSF resulted in significantly higher NET release at 48 h compared to untreated cells.					
35242132	9	51	with	incubation	1557:1566	arg1	G-CSF					1573:1577	G-CSF	1573:1577	G-CSF	1573:1577	Similarly, incubation with G-CSF resulted in significantly higher NET release at 48 h compared to untreated cells.					
35242132	10	52	theme	NE	1729:1730	arg1	activity					1717:1724	significantly higher enzymatic activity	1686:1724	significantly higher enzymatic activity of NE and MPO	1686:1738	Furthermore, NETs showed significantly higher enzymatic activity of NE and MPO after incubation with G-CSF.					
35242132	7	53	dep	48 h	1341:1344	arg1	to					1338:1339	to	1338:1339	to	1338:1339	RESULTS Untreated neutrophils could form NETs after stimulation with PMA for up to 24 h. Incubation with LPS extended their ability to form NETs for up to 48 h.					
35242132	12	54	theme	low	1947:1949	arg1	concentrations					1951:1964	low concentrations	1947:1964	low concentrations	1947:1964	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	9	55	theme	untreated	1644:1652	arg1	cells					1654:1658	untreated cells	1644:1658	untreated cells	1644:1658	Similarly, incubation with G-CSF resulted in significantly higher NET release at 48 h compared to untreated cells.					
35242132	7	56	with	Incubation	1275:1284	arg1	LPS					1291:1293	LPS	1291:1293	LPS extended their ability to form NETs for up to 48 h	1291:1344	RESULTS Untreated neutrophils could form NETs after stimulation with PMA for up to 24 h. Incubation with LPS extended their ability to form NETs for up to 48 h.					
35242132	10	57	theme	MPO	1736:1738	arg1	activity					1717:1724	significantly higher enzymatic activity	1686:1724	significantly higher enzymatic activity of NE and MPO	1686:1738	Furthermore, NETs showed significantly higher enzymatic activity of NE and MPO after incubation with G-CSF.					
35242132	9	58	from	48 h	1627:1630	arg1	release					1616:1622	significantly higher NET release	1591:1622	significantly higher NET release at 48 h compared to untreated cells	1591:1658	Similarly, incubation with G-CSF resulted in significantly higher NET release at 48 h compared to untreated cells.					
35242132	5	59	theme	culture	727:733	arg1	conditions					735:744	various culture conditions	719:744	various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α)	719:862	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	2	60	theme	neutrophil	237:246	arg1	granulocytes					248:259	neutrophil granulocytes	237:259	neutrophil granulocytes	237:259	Research on neutrophil granulocytes is limited because of their short lifetime of only a few hours.					
35242132	1	61	theme	tissue	210:215	arg1	repair					217:222	tissue repair	210:222	tissue repair	210:222	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	5	62	theme	neutrophil	684:693	arg1	survival					695:702	neutrophil survival	684:702	neutrophil survival	684:702	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	1	63	theme	innate	123:128	arg1	immunity-are					130:141	innate immunity-are	123:141	innate immunity-are	123:141	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	6	64	theme	myeloperoxidase	1042:1056	arg1	sandwich-ELISA					1068:1081	myeloperoxidase (MPO)-DNA sandwich-ELISA	1042:1081	myeloperoxidase (MPO)-DNA sandwich-ELISA	1042:1081	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	8	65	theme	untreated	1446:1454	arg1	cells					1456:1460	untreated cells	1446:1460	untreated cells	1446:1460	At 48 h, NET release of neutrophils cultured with LPS was significantly higher compared to that of untreated cells; however, no significantly different enzymatic activity of NE and MPO was observed.					
35242132	12	66	theme	considerable	2031:2042	arg1	differences					2044:2054	considerable differences	2031:2054	considerable differences in NET formation and composition	2031:2087	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	13	67	theme	novel	2210:2214	arg1	insights					2216:2223	novel insights	2210:2223	novel insights for neutrophil behaviour	2210:2248	These results provide new information for the use of neutrophils in long-term experiments for NET formation and provide novel insights for neutrophil behaviour under inflammatory conditions.					
35242132	7	68	theme	Untreated	1194:1202	arg1	neutrophils					1204:1214	Untreated neutrophils	1194:1214	Untreated neutrophils	1194:1214	RESULTS Untreated neutrophils could form NETs after stimulation with PMA for up to 24 h. Incubation with LPS extended their ability to form NETs for up to 48 h.					
35242132	6	69	theme	phorbol	972:978	arg1	PMA					1005:1007	PMA	1005:1007	PMA	1005:1007	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	6	69	theme	phorbol	972:978	arg1	13-acetate					993:1002	phorbol 12-myristate 13-acetate	972:1002	phorbol 12-myristate 13-acetate (PMA)	972:1008	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	1	70	theme	numerous	155:162	arg1	infection					182:190	infection	182:190	infection	182:190	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	1	70	theme	numerous	155:162	arg1	repair					217:222	tissue repair	210:222	tissue repair	210:222	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	1	70	theme	numerous	155:162	arg1	inflammation					193:204	inflammation	193:204	inflammation	193:204	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	1	70	theme	numerous	155:162	arg1	processes					164:172	numerous processes	155:172	numerous processes such as infection, inflammation and tissue repair	155:222	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	12	71	dep	CONCLUSIONS	1907:1917	arg1	CONCLUSIONS					1907:1917	CONCLUSIONS G-CSF, LPS or TNF-α	1907:1937	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations	1907:1964	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	12	71	dep	CONCLUSIONS	1907:1917	arg1	LPS					1926:1928	LPS	1926:1928	LPS	1926:1928	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	12	71	dep	CONCLUSIONS	1907:1917	arg1	each					1939:1942	each	1939:1942	each	1939:1942	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	12	71	dep	CONCLUSIONS	1907:1917	arg1	G-CSF					1919:1923	G-CSF	1919:1923	G-CSF	1919:1923	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	12	71	dep	CONCLUSIONS	1907:1917	arg1	TNF-α					1933:1937	TNF-α	1933:1937	TNF-α	1933:1937	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	12	72	theme	neutrophils	2005:2015	arg1	survival					1984:1991	prolonged survival	1974:1991	prolonged survival of cultured neutrophils	1974:2015	CONCLUSIONS G-CSF, LPS or TNF-α each at low concentrations lead to prolonged survival of cultured neutrophils, resulting in considerable differences in NET formation and composition.					
35242132	11	73	with	incubation	1777:1786	arg1	TNF-α					1793:1797	TNF-α	1793:1797	TNF-α	1793:1797	Lastly, incubation with TNF-α had no influence on NET release compared to untreated cells although survival counts were altered by TNF-α.					
35242132	1	74	theme	Neutrophil	59:68	arg1	swords					113:118	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords	48:118	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are	48:141	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	10	75	with	incubation	1746:1755	arg1	G-CSF					1762:1766	G-CSF	1762:1766	G-CSF	1762:1766	Furthermore, NETs showed significantly higher enzymatic activity of NE and MPO after incubation with G-CSF.					
35242132	4	76	theme	long-term	476:484	arg1	neutrophils					496:506	These long-term surviving neutrophils	470:506	These long-term surviving neutrophils	470:506	These long-term surviving neutrophils are reported to maintain phagocytic activity and cytokine release; however, little is known regarding their capability to release NETs.					
35242132	5	77	theme	colony-stimulating	764:781	arg1	G-CSF					791:795	G-CSF	791:795	G-CSF	791:795	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	5	77	theme	colony-stimulating	764:781	arg1	factor					783:788	granulocyte colony-stimulating factor	752:788	granulocyte colony-stimulating factor (G-CSF)	752:796	METHODS We analysed the prolongation of neutrophil survival in vitro under various culture conditions using granulocyte colony-stimulating factor (G-CSF), lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNF-α) by flow cytometry and a viability assay.					
35242132	8	78	theme	enzymatic	1499:1507	arg1	activity					1509:1516	no significantly different enzymatic activity	1472:1516	no significantly different enzymatic activity of NE and MPO	1472:1530	At 48 h, NET release of neutrophils cultured with LPS was significantly higher compared to that of untreated cells; however, no significantly different enzymatic activity of NE and MPO was observed.					
35242132	11	79	theme	survival	1868:1875	arg1	counts					1877:1882	survival counts	1868:1882	survival counts	1868:1882	Lastly, incubation with TNF-α had no influence on NET release compared to untreated cells although survival counts were altered by TNF-α.					
35242132	11	80	from	influence	1806:1814	arg1	release					1823:1829	NET release	1819:1829	NET release	1819:1829	Lastly, incubation with TNF-α had no influence on NET release compared to untreated cells although survival counts were altered by TNF-α.					
35242132	0	81	theme	Long-Term	15:23	arg1	Neutrophils					35:45	Long-Term Surviving Neutrophils	15:45	Long-Term Surviving Neutrophils	15:45	NET Release of Long-Term Surviving Neutrophils.					
35242132	1	82	theme	-as	96:98	arg1	swords					113:118	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords	48:118	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are	48:141	BACKGROUND Neutrophil extracellular traps (NETs)-as double-edged swords of innate immunity-are involved in numerous processes such as infection, inflammation and tissue repair.					
35242132	6	83	theme	-DNA	1063:1066	arg1	sandwich-ELISA					1068:1081	myeloperoxidase (MPO)-DNA sandwich-ELISA	1042:1081	myeloperoxidase (MPO)-DNA sandwich-ELISA	1042:1081	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	0	84	theme	Neutrophils	35:45	arg1	Release					4:10	NET Release	0:10	NET Release of Long-Term Surviving Neutrophils	0:45	NET Release of Long-Term Surviving Neutrophils.					
35242132	3	85	theme	promising	451:459	arg1	results					461:467	promising results	451:467	promising results	451:467	Several attempts have been made to prolong the half-life of neutrophils using cytokines and bacterial products and have shown promising results.					
35242132	13	86	theme	long-term	2158:2166	arg1	experiments					2168:2178	long-term experiments	2158:2178	long-term experiments	2158:2178	These results provide new information for the use of neutrophils in long-term experiments for NET formation and provide novel insights for neutrophil behaviour under inflammatory conditions.					
35242132	6	87	theme	neutrophil	1134:1143	arg1	NE					1155:1156	NE	1155:1156	NE	1155:1156	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	6	87	theme	neutrophil	1134:1143	arg1	elastase					1145:1152	neutrophil elastase	1134:1152	neutrophil elastase (NE)	1134:1157	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	6	87	theme	neutrophil	1134:1143	arg1	assays					1100:1105	fluorometric assays	1087:1105	fluorometric assays for cell-free DNA (cfDNA)	1087:1131	Additionally, we assessed NET formation following stimulation with phorbol 12-myristate 13-acetate (PMA) by immunofluorescence staining, myeloperoxidase (MPO)-DNA sandwich-ELISA and fluorometric assays for cell-free DNA (cfDNA), neutrophil elastase (NE) and myeloperoxidase (MPO).					
35242132	4	88	theme	cytokine	557:564	arg1	release					566:572	cytokine release	557:572	cytokine release	557:572	These long-term surviving neutrophils are reported to maintain phagocytic activity and cytokine release; however, little is known regarding their capability to release NETs.					
35242132	8	89	theme	neutrophils	1371:1381	arg1	release					1360:1366	NET release	1356:1366	NET release of neutrophils cultured with LPS	1356:1399	At 48 h, NET release of neutrophils cultured with LPS was significantly higher compared to that of untreated cells; however, no significantly different enzymatic activity of NE and MPO was observed.					
35242132	2	90	theme	hours	318:322	arg1	lifetime					295:302	their short lifetime	283:302	their short lifetime of only a few hours	283:322	Research on neutrophil granulocytes is limited because of their short lifetime of only a few hours.					
35242132	13	91	theme	neutrophils	2143:2153	arg1	use					2136:2138	the use	2132:2138	the use of neutrophils	2132:2153	These results provide new information for the use of neutrophils in long-term experiments for NET formation and provide novel insights for neutrophil behaviour under inflammatory conditions.					
36409543	1	0	theme	human	129:133	arg1	composition					140:150	human milk composition	129:150	human milk composition	129:150	Gestational diabetes mellitus (GDM) is known to affect human milk composition.					
36409543	7	1	dep	Weight-for-age	711:724	arg1	WAZ					727:729	WAZ	727:729	WAZ	727:729	Weight-for-age (WAZ) and weight-for-length z-scores were calculated.					
36409543	1	2	theme	milk	135:138	arg1	composition					140:150	human milk composition	129:150	human milk composition	129:150	Gestational diabetes mellitus (GDM) is known to affect human milk composition.					
36409543	13	3	dep	unexposed	1535:1543	arg1	children					1526:1533	children	1526:1533	children unexposed (GDM-) to GDM	1526:1557	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	13	3	dep	unexposed	1535:1543	arg1	GDM-					1546:1549	GDM-	1546:1549	GDM-	1546:1549	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	13	4	theme	%	1488:1488	arg1	CI					1490:1491	95% CI	1486:1491	95% CI	1486:1491	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	10	5	theme	GDM-	954:957	arg1	women					959:963	GDM- women	954:963	GDM- women	954:963	TG concentration was higher in GDM+ than in GDM- women (6.3 ± 2.0 versus 5.3 ± 1.2, p = 0.04).					
36409543	13	6	dep	exposed	1583:1589	arg1	children					1574:1581	children	1574:1581	children exposed (GDM+) In conclusion	1574:1610	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	13	6	dep	exposed	1583:1589	arg1	GDM+					1592:1595	GDM+	1592:1595	GDM+	1592:1595	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	12	7	dep	associated	1125:1134	arg1	r = -0.30					1178:1186	r = -0.30	1178:1186	r = -0.30	1178:1186	Maternal age was associated with TG (r = 0.28, p = 0.04) and lactose (r = -0.30, p = 0.03), while fasting glucose was associated with proteins (r = 0.30, p = 0.03) and tended to be associated with TG (r = 0.27, p = 0.05) and energy (r = 0.24, p = 0.08).					
36409543	10	8	dep	higher	931:936	arg1	5.3 ± 1.2					983:991	5.3 ± 1.2	983:991	5.3 ± 1.2	983:991	TG concentration was higher in GDM+ than in GDM- women (6.3 ± 2.0 versus 5.3 ± 1.2, p = 0.04).					
36409543	10	8	dep	higher	931:936	arg1	6.3 ± 2.0					966:974	6.3 ± 2.0	966:974	6.3 ± 2.0	966:974	TG concentration was higher in GDM+ than in GDM- women (6.3 ± 2.0 versus 5.3 ± 1.2, p = 0.04).					
36409543	11	9	theme	infant	1085:1090	arg1	p = 0.23					1097:1104	p = 0.23	1097:1104	p = 0.23	1097:1104	This difference was no longer significant after adjustment for maternal age and infant sex (p = 0.23).					
36409543	11	9	theme	infant	1085:1090	arg1	sex					1092:1094	infant sex	1085:1094	infant sex (p = 0.23)	1085:1105	This difference was no longer significant after adjustment for maternal age and infant sex (p = 0.23).					
36409543	14	10	from	TG	1719:1720	arg1	milk					1731:1734	human milk	1725:1734	human milk	1725:1734	Finally, TG in human milk was associated with infant growth among GDM- children but not among GDM+ children.					
36409543	13	11	theme	GDM	1613:1615	arg1	status					1617:1622	GDM status	1613:1622	GDM status	1613:1622	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	11	12	theme	maternal	1068:1075	arg1	age					1077:1079	maternal age	1068:1079	maternal age	1068:1079	This difference was no longer significant after adjustment for maternal age and infant sex (p = 0.23).					
36409543	13	13	dep	associated	1391:1400	arg1	0.50					1462:1465	0.50	1462:1465	0.50	1462:1465	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	13	13	dep	associated	1391:1400	arg1	WAZ					1472:1474	WAZ (β: 0.40	1472:1483	WAZ (β: 0.40	1472:1483	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	2	14	theme	infant	450:455	arg1	growth					457:462	infant growth	450:462	infant growth	450:462	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	13	15	theme	interval	1439:1446	arg1	[CI					1448:1450	β: 0.26, 95% confidence interval [CI	1415:1450	[CI	1448:1450	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	13	15	theme	interval	1439:1446	arg1	weight					1407:1412	weight	1407:1412	weight (β: 0.26, 95% confidence interval [CI]	1407:1451	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	2	16	theme	women	238:242	arg1	macronutrient					188:200	macronutrient	188:200	macronutrient	188:200	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	2	16	theme	women	238:242	arg1	content					213:219	energy content	206:219	energy content	206:219	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	4	17	theme	human	576:580	arg1	milk					582:585	human milk	576:585	human milk	576:585	Triglyceride (TG), lactose, and protein content of human milk were measured.					
36409543	14	18	theme	infant	1756:1761	arg1	growth					1763:1768	infant growth	1756:1768	infant growth among GDM- children but not among GDM+ children	1756:1816	Finally, TG in human milk was associated with infant growth among GDM- children but not among GDM+ children.					
36409543	2	19	theme	milk	408:411	arg1	macronutrient					413:425	human milk macronutrient and energy content	402:444	macronutrient	413:425	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	9	20	theme	energy	852:857	arg1	content					859:865	energy content	852:865	energy content of human milk	852:879	Protein, lactose, and energy content of human milk were similar between groups.					
36409543	9	21	theme	human	870:874	arg1	milk					876:879	human milk	870:879	human milk	870:879	Protein, lactose, and energy content of human milk were similar between groups.					
36409543	2	22	theme	maternal	314:321	arg1	health					323:328	maternal health	314:328	maternal health	314:328	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	0	23	theme	Diabetes	12:19	arg1	Mellitus					21:28	Gestational Diabetes Mellitus	0:28	Gestational Diabetes Mellitus	0:28	Gestational Diabetes Mellitus, Human Milk Composition, and Infant Growth.					
36409543	13	24	theme	human	1686:1690	arg1	composition					1697:1707	human milk composition	1686:1707	human milk composition	1686:1707	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	1	25	theme	Gestational	74:84	arg1	GDM					105:107	GDM	105:107	GDM	105:107	Gestational diabetes mellitus (GDM) is known to affect human milk composition.					
36409543	1	25	theme	Gestational	74:84	arg1	mellitus					95:102	Gestational diabetes mellitus	74:102	Gestational diabetes mellitus (GDM)	74:108	Gestational diabetes mellitus (GDM) is known to affect human milk composition.					
36409543	0	26	theme	Gestational	0:10	arg1	Mellitus					21:28	Gestational Diabetes Mellitus	0:28	Gestational Diabetes Mellitus	0:28	Gestational Diabetes Mellitus, Human Milk Composition, and Infant Growth.					
36409543	7	27	theme	Weight-for-age	711:724	arg1	z-scores					754:761	Weight-for-age (WAZ) and weight-for-length z-scores	711:761	Weight-for-age (WAZ) and weight-for-length z-scores	711:761	Weight-for-age (WAZ) and weight-for-length z-scores were calculated.					
36409543	13	28	theme	maternal	1625:1632	arg1	age					1634:1636	maternal age	1625:1636	maternal age	1625:1636	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	1	29	theme	diabetes	86:93	arg1	GDM					105:107	GDM	105:107	GDM	105:107	Gestational diabetes mellitus (GDM) is known to affect human milk composition.					
36409543	1	29	theme	diabetes	86:93	arg1	mellitus					95:102	Gestational diabetes mellitus	74:102	Gestational diabetes mellitus (GDM)	74:108	Gestational diabetes mellitus (GDM) is known to affect human milk composition.					
36409543	4	30	theme	milk	582:585	arg1	Triglyceride					525:536	Triglyceride	525:536	Triglyceride (TG)	525:541	Triglyceride (TG), lactose, and protein content of human milk were measured.					
36409543	4	30	theme	milk	582:585	arg1	content					565:571	protein content	557:571	protein content	557:571	Triglyceride (TG), lactose, and protein content of human milk were measured.					
36409543	4	30	theme	milk	582:585	arg1	TG					539:540	TG	539:540	TG	539:540	Triglyceride (TG), lactose, and protein content of human milk were measured.					
36409543	4	30	theme	milk	582:585	arg1	lactose					544:550	lactose	544:550	lactose	544:550	Triglyceride (TG), lactose, and protein content of human milk were measured.					
36409543	2	31	theme	energy	363:368	arg1	content					370:376	human milk macronutrient and energy content	334:376	content	370:376	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	5	32	theme	glucose	610:616	arg1	test					628:631	An oral glucose tolerance test	602:631	An oral glucose tolerance test	602:631	An oral glucose tolerance test was performed.					
36409543	13	33	theme	human	1375:1379	arg1	milk					1381:1384	human milk	1375:1384	human milk	1375:1384	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	7	34	theme	weight-for-length	736:752	arg1	z-scores					754:761	Weight-for-age (WAZ) and weight-for-length z-scores	711:761	Weight-for-age (WAZ) and weight-for-length z-scores	711:761	Weight-for-age (WAZ) and weight-for-length z-scores were calculated.					
36409543	12	35	theme	fasting	1206:1212	arg1	glucose					1214:1220	fasting glucose	1206:1220	fasting glucose	1206:1220	Maternal age was associated with TG (r = 0.28, p = 0.04) and lactose (r = -0.30, p = 0.03), while fasting glucose was associated with proteins (r = 0.30, p = 0.03) and tended to be associated with TG (r = 0.27, p = 0.05) and energy (r = 0.24, p = 0.08).					
36409543	5	36	theme	tolerance	618:626	arg1	test					628:631	An oral glucose tolerance test	602:631	An oral glucose tolerance test	602:631	An oral glucose tolerance test was performed.					
36409543	3	37	theme	hindmilk	492:499	arg1	samples					501:507	hindmilk samples	492:507	hindmilk samples	492:507	Two months after delivery, hindmilk samples were collected.					
36409543	2	38	theme	milk	340:343	arg1	macronutrient					345:357	human milk macronutrient and energy content	334:376	macronutrient	345:357	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	0	39	theme	Milk	37:40	arg1	Composition					42:52	Human Milk Composition	31:52	Human Milk Composition	31:52	Gestational Diabetes Mellitus, Human Milk Composition, and Infant Growth.					
36409543	12	40	theme	Maternal	1108:1115	arg1	age					1117:1119	Maternal age	1108:1119	Maternal age	1108:1119	Maternal age was associated with TG (r = 0.28, p = 0.04) and lactose (r = -0.30, p = 0.03), while fasting glucose was associated with proteins (r = 0.30, p = 0.03) and tended to be associated with TG (r = 0.27, p = 0.05) and energy (r = 0.24, p = 0.08).					
36409543	13	41	theme	%	1426:1426	arg1	[CI					1448:1450	β: 0.26, 95% confidence interval [CI	1415:1450	[CI	1448:1450	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	13	41	theme	%	1426:1426	arg1	weight					1407:1412	weight	1407:1412	weight (β: 0.26, 95% confidence interval [CI]	1407:1451	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	12	42	dep	r = 0.24	1341:1348	arg1	p = 0.08					1351:1358	p = 0.08	1351:1358	p = 0.08	1351:1358	Maternal age was associated with TG (r = 0.28, p = 0.04) and lactose (r = -0.30, p = 0.03), while fasting glucose was associated with proteins (r = 0.30, p = 0.03) and tended to be associated with TG (r = 0.27, p = 0.05) and energy (r = 0.24, p = 0.08).					
36409543	2	43	theme	energy	206:211	arg1	content					213:219	energy content	206:219	energy content	206:219	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	2	44	theme	human	334:338	arg1	macronutrient					345:357	human milk macronutrient and energy content	334:376	macronutrient	345:357	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	0	45	theme	Human	31:35	arg1	Composition					42:52	Human Milk Composition	31:52	Human Milk Composition	31:52	Gestational Diabetes Mellitus, Human Milk Composition, and Infant Growth.					
36409543	12	46	dep	associated	1289:1298	arg1	r = 0.24					1341:1348	r = 0.24	1341:1348	r = 0.24	1341:1348	Maternal age was associated with TG (r = 0.28, p = 0.04) and lactose (r = -0.30, p = 0.03), while fasting glucose was associated with proteins (r = 0.30, p = 0.03) and tended to be associated with TG (r = 0.27, p = 0.05) and energy (r = 0.24, p = 0.08).					
36409543	2	47	theme	human	402:406	arg1	macronutrient					413:425	human milk macronutrient and energy content	402:444	macronutrient	413:425	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	9	48	theme	milk	876:879	arg1	lactose					839:845	lactose	839:845	lactose	839:845	Protein, lactose, and energy content of human milk were similar between groups.					
36409543	9	48	theme	milk	876:879	arg1	Protein					830:836	Protein	830:836	Protein	830:836	Protein, lactose, and energy content of human milk were similar between groups.					
36409543	9	48	theme	milk	876:879	arg1	content					859:865	energy content	852:865	energy content of human milk	852:879	Protein, lactose, and energy content of human milk were similar between groups.					
36409543	4	49	theme	protein	557:563	arg1	content					565:571	protein content	557:571	protein content	557:571	Triglyceride (TG), lactose, and protein content of human milk were measured.					
36409543	10	50	theme	TG	910:911	arg1	concentration					913:925	TG concentration	910:925	TG concentration	910:925	TG concentration was higher in GDM+ than in GDM- women (6.3 ± 2.0 versus 5.3 ± 1.2, p = 0.04).					
36409543	12	51	dep	TG	1141:1142	arg1	r = 0.28					1145:1152	r = 0.28	1145:1152	r = 0.28	1145:1152	Maternal age was associated with TG (r = 0.28, p = 0.04) and lactose (r = -0.30, p = 0.03), while fasting glucose was associated with proteins (r = 0.30, p = 0.03) and tended to be associated with TG (r = 0.27, p = 0.05) and energy (r = 0.24, p = 0.08).					
36409543	13	52	theme	fasting	1643:1649	arg1	level					1659:1663	fasting glucose level	1643:1663	fasting glucose level	1643:1663	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	12	53	dep	associated	1226:1235	arg1	r = 0.30					1252:1259	r = 0.30	1252:1259	r = 0.30	1252:1259	Maternal age was associated with TG (r = 0.28, p = 0.04) and lactose (r = -0.30, p = 0.03), while fasting glucose was associated with proteins (r = 0.30, p = 0.03) and tended to be associated with TG (r = 0.27, p = 0.05) and energy (r = 0.24, p = 0.08).					
36409543	13	54	theme	confidence	1428:1437	arg1	[CI					1448:1450	β: 0.26, 95% confidence interval [CI	1415:1450	[CI	1448:1450	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	13	54	theme	confidence	1428:1437	arg1	weight					1407:1412	weight	1407:1412	weight (β: 0.26, 95% confidence interval [CI]	1407:1451	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	10	55	dep	6.3 ± 2.0	966:974	arg1	p = 0.04					994:1001	p = 0.04	994:1001	p = 0.04	994:1001	TG concentration was higher in GDM+ than in GDM- women (6.3 ± 2.0 versus 5.3 ± 1.2, p = 0.04).					
36409543	13	56	theme	TG	1362:1363	arg1	levels					1365:1370	TG levels	1362:1370	TG levels in human milk	1362:1384	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	13	57	theme	glucose	1651:1657	arg1	level					1659:1663	fasting glucose level	1643:1663	fasting glucose level	1643:1663	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	3	58	dep	months	469:474	arg1	delivery					482:489	delivery	482:489	delivery	482:489	Two months after delivery, hindmilk samples were collected.					
36409543	2	59	theme	milk	230:233	arg1	macronutrient					188:200	macronutrient	188:200	macronutrient	188:200	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	2	59	theme	milk	230:233	arg1	content					213:219	energy content	206:219	energy content	206:219	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	6	60	from	birth	676:680	arg1	length					666:671	length	666:671	length	666:671	Infant weight and length at birth and 2 months were collected.					
36409543	6	60	from	birth	676:680	arg1	weight					655:660	Infant weight	648:660	Infant weight	648:660	Infant weight and length at birth and 2 months were collected.					
36409543	13	61	dep	0.75	1502:1505	arg1	to					1499:1500	to	1499:1500	to	1499:1500	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	2	62	with	women	238:242	arg1	GDM+					250:253	(GDM+)	249:254	(GDM+)	249:254	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	5	63	theme	oral	605:608	arg1	test					628:631	An oral glucose tolerance test	602:631	An oral glucose tolerance test	602:631	An oral glucose tolerance test was performed.					
36409543	2	64	theme	study	166:170	arg1	Aims					153:156	Aims	153:156	Aims of this study	153:170	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	2	65	theme	human	224:228	arg1	milk					230:233	human milk	224:233	human milk	224:233	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	8	66	theme	GDM-	804:807	arg1	women					809:813	29 GDM- women	801:813	29 GDM- women	801:813	Twenty-four GDM+ and 29 GDM- women were included.					
36409543	13	67	from	levels	1365:1370	arg1	milk					1381:1384	human milk	1375:1384	human milk	1375:1384	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	14	68	theme	GDM+	1804:1807	arg1	children					1809:1816	GDM+ children	1804:1816	GDM+ children	1804:1816	Finally, TG in human milk was associated with infant growth among GDM- children but not among GDM+ children.					
36409543	0	69	theme	Infant	59:64	arg1	Growth					66:71	Infant Growth	59:71	Infant Growth	59:71	Gestational Diabetes Mellitus, Human Milk Composition, and Infant Growth.					
36409543	6	70	from	months	688:693	arg1	length					666:671	length	666:671	length	666:671	Infant weight and length at birth and 2 months were collected.					
36409543	6	70	from	months	688:693	arg1	weight					655:660	Infant weight	648:660	Infant weight	648:660	Infant weight and length at birth and 2 months were collected.					
36409543	14	71	theme	GDM-	1776:1779	arg1	children					1781:1788	GDM- children	1776:1788	GDM- children	1776:1788	Finally, TG in human milk was associated with infant growth among GDM- children but not among GDM+ children.					
36409543	6	72	theme	Infant	648:653	arg1	weight					655:660	Infant weight	648:660	Infant weight	648:660	Infant weight and length at birth and 2 months were collected.					
36409543	12	73	dep	r = 0.30	1252:1259	arg1	p = 0.03					1262:1269	p = 0.03	1262:1269	p = 0.03	1262:1269	Maternal age was associated with TG (r = 0.28, p = 0.04) and lactose (r = -0.30, p = 0.03), while fasting glucose was associated with proteins (r = 0.30, p = 0.03) and tended to be associated with TG (r = 0.27, p = 0.05) and energy (r = 0.24, p = 0.08).					
36409543	12	74	dep	r = -0.30	1178:1186	arg1	p = 0.03					1189:1196	p = 0.03	1189:1196	p = 0.03	1189:1196	Maternal age was associated with TG (r = 0.28, p = 0.04) and lactose (r = -0.30, p = 0.03), while fasting glucose was associated with proteins (r = 0.30, p = 0.03) and tended to be associated with TG (r = 0.27, p = 0.05) and energy (r = 0.24, p = 0.08).					
36409543	12	75	dep	TG	1305:1306	arg1	r = 0.27					1309:1316	r = 0.27	1309:1316	r = 0.27	1309:1316	Maternal age was associated with TG (r = 0.28, p = 0.04) and lactose (r = -0.30, p = 0.03), while fasting glucose was associated with proteins (r = 0.30, p = 0.03) and tended to be associated with TG (r = 0.27, p = 0.05) and energy (r = 0.24, p = 0.08).					
36409543	2	76	theme	energy	431:436	arg1	content					438:444	human milk macronutrient and energy content	402:444	content	438:444	Aims of this study were to compare macronutrient and energy content of human milk of women with (GDM+) and without GDM (GDM-), to assess the association between maternal health and human milk macronutrient and energy content and association between human milk macronutrient and energy content and infant growth.					
36409543	13	77	theme	milk	1692:1695	arg1	composition					1697:1707	human milk composition	1686:1707	human milk composition	1686:1707	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	13	78	dep	0.50	1462:1465	arg1	to					1459:1460	to	1459:1460	to	1459:1460	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	13	78	dep	0.50	1462:1465	arg1	CI					1490:1491	95% CI	1486:1491	95% CI	1486:1491	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	13	78	dep	0.50	1462:1465	arg1	0.75					1502:1505	0.75	1502:1505	0.75	1502:1505	TG levels in human milk were associated with weight (β: 0.26, 95% confidence interval [CI]: 0.02 to 0.50) and WAZ (β: 0.40, 95% CI: 0.05 to 0.75) at 2 months among children unexposed (GDM-) to GDM, but not among children exposed (GDM+) In conclusion, GDM status, maternal age, and fasting glucose level were associated with human milk composition.					
36409543	14	79	theme	human	1725:1729	arg1	milk					1731:1734	human milk	1725:1734	human milk	1725:1734	Finally, TG in human milk was associated with infant growth among GDM- children but not among GDM+ children.					
36996661	4	0	theme	uptake	956:961	arg1	process					963:969	the water uptake process	946:969	the water uptake process that occurs over time with various kinetics	946:1013	In this work, the main purpose of MCR is to provide spectral signatures and distribution maps of the components involved in the water uptake process that occurs over time with various kinetics.					
36996661	6	1	theme	physico-chemical	1481:1496	arg1	point					1498:1502	a biological and physico-chemical point	1464:1502	a biological and physico-chemical point of view	1464:1510	The results were supplemented by scanning electron microscopy (SEM) observations in order to interpret these two waterfronts in a biological and physico-chemical point of view.					
36996661	1	2	theme	microimaging	167:178	arg1	resonance					157:165	Magnetic resonance microimaging	148:178	Magnetic resonance microimaging (MRμI)	148:185	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	1	2	theme	microimaging	167:178	arg1	MRμI					181:184	MRμI	181:184	MRμI	181:184	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	1	2	theme	microimaging	167:178	arg1	technique					205:213	an outstanding technique	190:213	an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner	190:322	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	6	3	theme	scanning	1369:1376	arg1	SEM					1399:1401	SEM	1399:1401	SEM	1399:1401	The results were supplemented by scanning electron microscopy (SEM) observations in order to interpret these two waterfronts in a biological and physico-chemical point of view.					
36996661	6	3	theme	scanning	1369:1376	arg1	microscopy					1387:1396	scanning electron microscopy	1369:1396	scanning electron microscopy (SEM) observations in order to interpret these two waterfronts in a biological and physico-chemical point of view	1369:1510	The results were supplemented by scanning electron microscopy (SEM) observations in order to interpret these two waterfronts in a biological and physico-chemical point of view.					
36996661	1	4	theme	bio-based	259:267	arg1	materials					269:277	millimetric bio-based materials	247:277	millimetric bio-based materials	247:277	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	2	5	theme	analysis	490:497	arg1	tools					499:503	reliable image processing and analysis tools	460:503	tools	499:503	However, depending on the composition of the material, monitoring and quantification of these transfers can be very complex, and hence reliable image processing and analysis tools are necessary.					
36996661	3	6	theme	starch	682:687	arg1	blend					698:702	a potato starch extruded blend	673:702	a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications	673:819	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	4	7	theme	main	840:843	arg1	purpose					845:851	the main purpose	836:851	the main purpose of MCR	836:858	In this work, the main purpose of MCR is to provide spectral signatures and distribution maps of the components involved in the water uptake process that occurs over time with various kinetics.					
36996661	3	8	dep	curve	574:578	arg1	squares					609:615	resolution-alternating least squares	580:615	multivariate curve resolution-alternating least squares (MCR-ALS)	561:625	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	3	8	dep	curve	574:578	arg1	MCR-ALS					618:624	MCR-ALS	618:624	MCR-ALS	618:624	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	5	9	dep	allowed	1030:1036	arg1	permitted					1132:1140	permitted	1132:1140	permitted	1132:1140	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	0	10	theme	resonance	95:103	arg1	imaging					105:111	magnetic resonance imaging	86:111	magnetic resonance imaging	86:111	Monitoring of water sorption and swelling of potato starch-glycerol extruded blend by magnetic resonance imaging and multivariate curve resolution.					
36996661	3	11	theme	extruded	689:696	arg1	blend					698:702	a potato starch extruded blend	673:702	a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications	673:819	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	4	12	theme	MCR	856:858	arg1	purpose					845:851	the main purpose	836:851	the main purpose of MCR	836:858	In this work, the main purpose of MCR is to provide spectral signatures and distribution maps of the components involved in the water uptake process that occurs over time with various kinetics.					
36996661	5	13	theme	different	1184:1192	arg1	times					1194:1198	two different times	1180:1198	two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI)	1180:1333	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	3	14	theme	food	803:806	arg1	applications					808:819	biomedical, textile, and food applications	778:819	biomedical, textile, and food applications	778:819	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	0	15	theme	magnetic	86:93	arg1	imaging					105:111	magnetic resonance imaging	86:111	magnetic resonance imaging	86:111	Monitoring of water sorption and swelling of potato starch-glycerol extruded blend by magnetic resonance imaging and multivariate curve resolution.					
36996661	5	16	theme	system	1061:1066	arg1	evolution					1068:1076	the system evolution	1057:1076	the system evolution	1057:1076	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	5	17	theme	waterfronts	1164:1174	arg1	resolution					1146:1155	the resolution	1142:1155	the resolution of two waterfronts	1142:1174	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	2	18	theme	material	370:377	arg1	composition					351:361	the composition	347:361	the composition of the material	347:377	However, depending on the composition of the material, monitoring and quantification of these transfers can be very complex, and hence reliable image processing and analysis tools are necessary.					
36996661	5	19	theme	evolution	1068:1076	arg1	description					1042:1052	the description	1038:1052	the description of the system evolution	1038:1076	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	4	20	with	time	988:991	arg1	kinetics					1006:1013	various kinetics	998:1013	various kinetics	998:1013	In this work, the main purpose of MCR is to provide spectral signatures and distribution maps of the components involved in the water uptake process that occurs over time with various kinetics.					
36996661	4	21	theme	components	923:932	arg1	signatures					883:892	spectral signatures	874:892	spectral signatures	874:892	In this work, the main purpose of MCR is to provide spectral signatures and distribution maps of the components involved in the water uptake process that occurs over time with various kinetics.					
36996661	4	21	theme	components	923:932	arg1	maps					911:914	distribution maps	898:914	distribution maps	898:914	In this work, the main purpose of MCR is to provide spectral signatures and distribution maps of the components involved in the water uptake process that occurs over time with various kinetics.					
36996661	3	22	theme	20	715:716	arg1	%					717:717	%	717:717	%	717:717	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	5	23	theme	local	1104:1108	arg1	level					1118:1122	a global (image) and a local (pixel) level	1081:1122	level	1118:1122	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	3	24	theme	%	717:717	arg1	glycerol					719:726	20% glycerol	715:726	20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications	715:819	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	5	25	theme	pixel	1111:1115	arg1	level					1118:1122	a global (image) and a local (pixel) level	1081:1122	level	1118:1122	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	3	26	theme	multivariate	561:572	arg1	curve					574:578	multivariate curve resolution-alternating least squares (MCR-ALS)	561:625	multivariate curve resolution-alternating least squares (MCR-ALS)	561:625	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	5	27	theme	other	1249:1253	arg1	method					1279:1284	any other mathematical processing method	1245:1284	any other mathematical processing method usually used in magnetic resonance imaging (MRI)	1245:1333	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	1	28	theme	non-destructive	284:298	arg1	manner					317:322	a non-destructive and non-invasive manner	282:322	a non-destructive and non-invasive manner	282:322	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	3	29	theme	curve	574:578	arg1	combination					537:547	a combination	535:547	a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS)	535:625	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	6	30	theme	biological	1466:1475	arg1	point					1498:1502	a biological and physico-chemical point	1464:1502	a biological and physico-chemical point of view	1464:1510	The results were supplemented by scanning electron microscopy (SEM) observations in order to interpret these two waterfronts in a biological and physico-chemical point of view.					
36996661	0	31	theme	sorption	20:27	arg1	swelling					33:40	swelling	33:40	swelling of potato starch-glycerol	33:66	Monitoring of water sorption and swelling of potato starch-glycerol extruded blend by magnetic resonance imaging and multivariate curve resolution.					
36996661	0	31	theme	sorption	20:27	arg1	Monitoring					0:9	Monitoring	0:9	Monitoring of water sorption	0:27	Monitoring of water sorption and swelling of potato starch-glycerol extruded blend by magnetic resonance imaging and multivariate curve resolution.					
36996661	0	32	theme	curve	130:134	arg1	resolution					136:145	multivariate curve resolution	117:145	multivariate curve resolution	117:145	Monitoring of water sorption and swelling of potato starch-glycerol extruded blend by magnetic resonance imaging and multivariate curve resolution.					
36996661	3	33	contain	have	746:749	arg2	properties					763:772	interesting properties	751:772	interesting properties	751:772	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	3	33	contain	have	746:749	arg1	glycerol					719:726	20% glycerol	715:726	20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications	715:819	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	1	34	theme	outstanding	193:203	arg1	technique					205:213	an outstanding technique	190:213	an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner	190:322	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	1	34	theme	outstanding	193:203	arg1	resonance					157:165	Magnetic resonance microimaging	148:178	Magnetic resonance microimaging (MRμI)	148:185	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	0	35	theme	water	14:18	arg1	sorption					20:27	water sorption	14:27	water sorption	14:27	Monitoring of water sorption and swelling of potato starch-glycerol extruded blend by magnetic resonance imaging and multivariate curve resolution.					
36996661	1	36	theme	non-invasive	304:315	arg1	manner					317:322	a non-destructive and non-invasive manner	282:322	a non-destructive and non-invasive manner	282:322	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	0	37	theme	multivariate	117:128	arg1	resolution					136:145	multivariate curve resolution	117:145	multivariate curve resolution	117:145	Monitoring of water sorption and swelling of potato starch-glycerol extruded blend by magnetic resonance imaging and multivariate curve resolution.					
36996661	3	38	theme	water	654:658	arg1	ingress					660:666	the water ingress	650:666	the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications	650:819	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	4	39	theme	water	950:954	arg1	process					963:969	the water uptake process	946:969	the water uptake process that occurs over time with various kinetics	946:1013	In this work, the main purpose of MCR is to provide spectral signatures and distribution maps of the components involved in the water uptake process that occurs over time with various kinetics.					
36996661	4	40	theme	distribution	898:909	arg1	maps					911:914	distribution maps	898:914	distribution maps	898:914	In this work, the main purpose of MCR is to provide spectral signatures and distribution maps of the components involved in the water uptake process that occurs over time with various kinetics.					
36996661	3	41	contain	containing	704:713	arg1	blend					698:702	a potato starch extruded blend	673:702	a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications	673:819	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	3	41	contain	containing	704:713	arg2	glycerol					719:726	20% glycerol	715:726	20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications	715:819	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	2	42	theme	transfers	419:427	arg1	quantification					395:408	quantification	395:408	quantification	395:408	However, depending on the composition of the material, monitoring and quantification of these transfers can be very complex, and hence reliable image processing and analysis tools are necessary.					
36996661	2	42	theme	transfers	419:427	arg1	monitoring					380:389	monitoring	380:389	monitoring	380:389	However, depending on the composition of the material, monitoring and quantification of these transfers can be very complex, and hence reliable image processing and analysis tools are necessary.					
36996661	0	43	theme	potato	45:50	arg1	starch-glycerol					52:66	potato starch-glycerol	45:66	potato starch-glycerol	45:66	Monitoring of water sorption and swelling of potato starch-glycerol extruded blend by magnetic resonance imaging and multivariate curve resolution.					
36996661	3	44	theme	biomedical	778:787	arg1	applications					808:819	biomedical, textile, and food applications	778:819	biomedical, textile, and food applications	778:819	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	0	45	theme	starch-glycerol	52:66	arg1	swelling					33:40	swelling	33:40	swelling of potato starch-glycerol	33:66	Monitoring of water sorption and swelling of potato starch-glycerol extruded blend by magnetic resonance imaging and multivariate curve resolution.					
36996661	0	45	theme	starch-glycerol	52:66	arg1	Monitoring					0:9	Monitoring	0:9	Monitoring of water sorption	0:27	Monitoring of water sorption and swelling of potato starch-glycerol extruded blend by magnetic resonance imaging and multivariate curve resolution.					
36996661	3	46	theme	resolution-alternating	580:601	arg1	squares					609:615	resolution-alternating least squares	580:615	multivariate curve resolution-alternating least squares (MCR-ALS)	561:625	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	3	46	theme	resolution-alternating	580:601	arg1	MCR-ALS					618:624	MCR-ALS	618:624	MCR-ALS	618:624	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	2	47	theme	reliable	460:467	arg1	processing					475:484	reliable image processing and analysis tools	460:503	processing	475:484	However, depending on the composition of the material, monitoring and quantification of these transfers can be very complex, and hence reliable image processing and analysis tools are necessary.					
36996661	3	48	theme	interesting	751:761	arg1	properties					763:772	interesting properties	751:772	interesting properties	751:772	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	5	49	theme	global	1083:1088	arg1	image					1091:1095	a global (image) and a local (pixel) level	1081:1122	image	1091:1095	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	3	50	theme	least	603:607	arg1	squares					609:615	resolution-alternating least squares	580:615	multivariate curve resolution-alternating least squares (MCR-ALS)	561:625	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	3	50	theme	least	603:607	arg1	MCR-ALS					618:624	MCR-ALS	618:624	MCR-ALS	618:624	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	5	51	theme	magnetic	1302:1309	arg1	MRI					1330:1332	MRI	1330:1332	MRI	1330:1332	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	5	51	theme	magnetic	1302:1309	arg1	imaging					1321:1327	magnetic resonance imaging	1302:1327	magnetic resonance imaging (MRI)	1302:1333	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	4	52	theme	spectral	874:881	arg1	signatures					883:892	spectral signatures	874:892	spectral signatures	874:892	In this work, the main purpose of MCR is to provide spectral signatures and distribution maps of the components involved in the water uptake process that occurs over time with various kinetics.					
36996661	3	53	theme	potato	675:680	arg1	blend					698:702	a potato starch extruded blend	673:702	a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications	673:819	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	5	54	theme	resonance	1311:1319	arg1	MRI					1330:1332	MRI	1330:1332	MRI	1330:1332	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	5	54	theme	resonance	1311:1319	arg1	imaging					1321:1327	magnetic resonance imaging	1302:1327	magnetic resonance imaging (MRI)	1302:1333	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	3	55	theme	MRμI	552:555	arg1	combination					537:547	a combination	535:547	a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS)	535:625	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	1	56	theme	water	228:232	arg1	transfers					234:242	water transfers	228:242	water transfers in millimetric bio-based materials	228:277	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	1	57	from	transfers	234:242	arg1	materials					269:277	millimetric bio-based materials	247:277	millimetric bio-based materials	247:277	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	6	58	theme	view	1507:1510	arg1	point					1498:1502	a biological and physico-chemical point	1464:1502	a biological and physico-chemical point of view	1464:1510	The results were supplemented by scanning electron microscopy (SEM) observations in order to interpret these two waterfronts in a biological and physico-chemical point of view.					
36996661	5	59	theme	mathematical	1255:1266	arg1	method					1279:1284	any other mathematical processing method	1245:1284	any other mathematical processing method usually used in magnetic resonance imaging (MRI)	1245:1333	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	4	60	theme	various	998:1004	arg1	kinetics					1006:1013	various kinetics	998:1013	various kinetics	998:1013	In this work, the main purpose of MCR is to provide spectral signatures and distribution maps of the components involved in the water uptake process that occurs over time with various kinetics.					
36996661	5	61	theme	processing	1268:1277	arg1	method					1279:1284	any other mathematical processing method	1245:1284	any other mathematical processing method usually used in magnetic resonance imaging (MRI)	1245:1333	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	1	62	theme	Magnetic	148:155	arg1	resonance					157:165	Magnetic resonance microimaging	148:178	Magnetic resonance microimaging (MRμI)	148:185	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	1	62	theme	Magnetic	148:155	arg1	MRμI					181:184	MRμI	181:184	MRμI	181:184	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	1	62	theme	Magnetic	148:155	arg1	technique					205:213	an outstanding technique	190:213	an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner	190:322	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36996661	6	63	theme	microscopy	1387:1396	arg1	observations					1404:1415	scanning electron microscopy (SEM) observations	1369:1415	scanning electron microscopy (SEM) observations in order to interpret these two waterfronts in a biological and physico-chemical point of view	1369:1510	The results were supplemented by scanning electron microscopy (SEM) observations in order to interpret these two waterfronts in a biological and physico-chemical point of view.					
36996661	2	64	theme	image	469:473	arg1	processing					475:484	reliable image processing and analysis tools	460:503	processing	475:484	However, depending on the composition of the material, monitoring and quantification of these transfers can be very complex, and hence reliable image processing and analysis tools are necessary.					
36996661	5	65	theme	a	1102:1102	arg1	level					1118:1122	a global (image) and a local (pixel) level	1081:1122	level	1118:1122	This approach allowed the description of the system evolution at a global (image) and a local (pixel) level, hence, permitted the resolution of two waterfronts, at two different times into the blend that could not be resolved by any other mathematical processing method usually used in magnetic resonance imaging (MRI).					
36996661	3	66	theme	textile	790:796	arg1	applications					808:819	biomedical, textile, and food applications	778:819	biomedical, textile, and food applications	778:819	In this study, a combination of MRμI and multivariate curve resolution-alternating least squares (MCR-ALS) is proposed to monitor the water ingress into a potato starch extruded blend containing 20% glycerol that was shown to have interesting properties for biomedical, textile, and food applications.					
36996661	6	67	theme	electron	1378:1385	arg1	SEM					1399:1401	SEM	1399:1401	SEM	1399:1401	The results were supplemented by scanning electron microscopy (SEM) observations in order to interpret these two waterfronts in a biological and physico-chemical point of view.					
36996661	6	67	theme	electron	1378:1385	arg1	microscopy					1387:1396	scanning electron microscopy	1369:1396	scanning electron microscopy (SEM) observations in order to interpret these two waterfronts in a biological and physico-chemical point of view	1369:1510	The results were supplemented by scanning electron microscopy (SEM) observations in order to interpret these two waterfronts in a biological and physico-chemical point of view.					
36996661	1	68	theme	millimetric	247:257	arg1	materials					269:277	millimetric bio-based materials	247:277	millimetric bio-based materials	247:277	Magnetic resonance microimaging (MRμI) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner.					
36979370	8	0	theme	polymer	1078:1084	arg1	distribution					1094:1105	polymer spatial distribution	1078:1105	polymer spatial distribution	1078:1105	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	0	theme	polymer	1078:1084	arg1	architecture					1064:1075	the architecture	1060:1075	the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers	1060:1149	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	1	theme	different	1208:1216	arg1	category					1234:1241	a different cell wall layer category	1206:1241	a different cell wall layer category based upon ontogenetical and chemical composition parameters	1206:1302	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	1	2	theme	tilted	206:211	arg1	plants					218:223	tilted flax plants	206:223	tilted flax plants	206:223	In a response to gravitropic stress, G-layers (gelatinous layers) were deposited in xylem cell walls of tilted flax plants.					
36979370	5	3	theme	Discriminating	697:710	arg1	bands					712:716	Discriminating bands	697:716	Discriminating bands	697:716	Discriminating bands were observed for cellulose (380-1150-1376 cm-1), hemicelluloses (517-1094-1126-1452 cm-1) and aromatics (1270-1599-1658 cm-1).					
36979370	0	4	from	Analysis	12:19	arg1	Walls					62:66	Bast Fiber and Xylem Cell Walls	36:66	Walls	62:66	Comparative Analysis of G-Layers in Bast Fiber and Xylem Cell Walls in Flax Using Raman Spectroscopy.					
36979370	0	4	from	Analysis	12:19	arg1	Fiber					41:45	Bast Fiber and Xylem Cell Walls	36:66	Fiber	41:45	Comparative Analysis of G-Layers in Bast Fiber and Xylem Cell Walls in Flax Using Raman Spectroscopy.					
36979370	2	5	dep	produced	240:247	arg1	side					277:280	side	277:280	side	277:280	G-layers were produced in both tension wood (upper side) as expected but were also observed in opposite wood (lower side).					
36979370	1	6	theme	flax	213:216	arg1	plants					218:223	tilted flax plants	206:223	tilted flax plants	206:223	In a response to gravitropic stress, G-layers (gelatinous layers) were deposited in xylem cell walls of tilted flax plants.					
36979370	1	7	theme	plants	218:223	arg1	walls					197:201	xylem cell walls	186:201	xylem cell walls of tilted flax plants	186:223	In a response to gravitropic stress, G-layers (gelatinous layers) were deposited in xylem cell walls of tilted flax plants.					
36979370	4	8	theme	fiber	661:665	arg1	G-layers					667:674	bast fiber G-layers	656:674	bast fiber G-layers	656:674	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	3	9	from	side	436:439	arg1	G-layers					397:404	xylem G-layers	391:404	xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions	391:516	Raman spectral profiles were acquired for xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions.					
36979370	4	10	theme	xylem	681:685	arg1	G-layers					687:694	xylem G-layers	681:694	xylem G-layers	681:694	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	8	11	theme	cell	1218:1221	arg1	category					1234:1241	a different cell wall layer category	1206:1241	a different cell wall layer category based upon ontogenetical and chemical composition parameters	1206:1302	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	0	12	from	Fiber	41:45	arg1	Flax					71:74	Flax	71:74	Flax	71:74	Comparative Analysis of G-Layers in Bast Fiber and Xylem Cell Walls in Flax Using Raman Spectroscopy.					
36979370	4	13	theme	principal	543:551	arg1	analysis					563:570	principal component analysis	543:570	principal component analysis (PCA)	543:576	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	4	13	theme	principal	543:551	arg1	PCA					573:575	PCA	573:575	PCA	573:575	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	4	14	theme	partial	582:588	arg1	analysis					616:623	partial least square-discriminant analysis	582:623	partial least square-discriminant analysis (PLS-DA)	582:632	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	4	14	theme	partial	582:588	arg1	PLS-DA					626:631	PLS-DA	626:631	PLS-DA	626:631	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	3	15	theme	spectral	355:362	arg1	profiles					364:371	Raman spectral profiles	349:371	Raman spectral profiles	349:371	Raman spectral profiles were acquired for xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions.					
36979370	0	16	theme	Raman	82:86	arg1	Spectroscopy					88:99	Raman Spectroscopy	82:99	Raman Spectroscopy	82:99	Comparative Analysis of G-Layers in Bast Fiber and Xylem Cell Walls in Flax Using Raman Spectroscopy.					
36979370	8	17	theme	chemical	1272:1279	arg1	composition					1281:1291	chemical composition	1272:1291	chemical composition	1272:1291	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	18	theme	bast	1111:1114	arg1	G-layers					1123:1130	G-layers	1123:1130	G-layers	1123:1130	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	18	theme	bast	1111:1114	arg1	G-layers					1142:1149	xylem G-layers	1136:1149	xylem G-layers	1136:1149	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	18	theme	bast	1111:1114	arg1	fibers					1116:1121	bast fibers G-layers and xylem G-layers	1111:1149	bast fibers G-layers and xylem G-layers	1111:1149	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	2	19	theme	opposite	321:328	arg1	wood					330:333	opposite wood	321:333	opposite wood (lower side)	321:346	G-layers were produced in both tension wood (upper side) as expected but were also observed in opposite wood (lower side).					
36979370	8	20	theme	fibers	1116:1121	arg1	distribution					1094:1105	polymer spatial distribution	1078:1105	polymer spatial distribution	1078:1105	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	20	theme	fibers	1116:1121	arg1	architecture					1064:1075	the architecture	1060:1075	the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers	1060:1149	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	20	theme	fibers	1116:1121	arg1	similar					1155:1161	similar	1155:1161	similar	1155:1161	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	3	21	theme	bast	472:475	arg1	fibers					477:482	bast fibers	472:482	bast fibers grown under non-tilted conditions	472:516	Raman spectral profiles were acquired for xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions.					
36979370	1	22	theme	gelatinous	149:158	arg1	G-layers					139:146	G-layers	139:146	G-layers (gelatinous layers)	139:166	In a response to gravitropic stress, G-layers (gelatinous layers) were deposited in xylem cell walls of tilted flax plants.					
36979370	1	22	theme	gelatinous	149:158	arg1	layers					160:165	gelatinous layers	149:165	gelatinous layers	149:165	In a response to gravitropic stress, G-layers (gelatinous layers) were deposited in xylem cell walls of tilted flax plants.					
36979370	0	23	theme	Comparative	0:10	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of G-Layers in Bast Fiber and Xylem Cell Walls in Flax	0:74	Comparative Analysis of G-Layers in Bast Fiber and Xylem Cell Walls in Flax Using Raman Spectroscopy.					
36979370	3	24	theme	fibers	477:482	arg1	G-layer					461:467	the G-layer	457:467	the G-layer of bast fibers grown under non-tilted conditions	457:516	Raman spectral profiles were acquired for xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions.					
36979370	3	25	theme	Raman	349:353	arg1	profiles					364:371	Raman spectral profiles	349:371	Raman spectral profiles	349:371	Raman spectral profiles were acquired for xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions.					
36979370	0	26	theme	G-Layers	24:31	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of G-Layers in Bast Fiber and Xylem Cell Walls in Flax	0:74	Comparative Analysis of G-Layers in Bast Fiber and Xylem Cell Walls in Flax Using Raman Spectroscopy.					
36979370	6	27	theme	tension/opposite-wood	892:912	arg1	sides					914:918	tension/opposite-wood sides	892:918	tension/opposite-wood sides	892:918	PCA did not allow separation of G-layers from tension/opposite-wood sides.					
36979370	2	28	theme	tension	257:263	arg1	wood					265:268	both tension wood	252:268	both tension wood	252:268	G-layers were produced in both tension wood (upper side) as expected but were also observed in opposite wood (lower side).					
36979370	4	29	theme	component	553:561	arg1	analysis					563:570	principal component analysis	543:570	principal component analysis (PCA)	543:576	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	4	29	theme	component	553:561	arg1	PCA					573:575	PCA	573:575	PCA	573:575	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	4	30	theme	bast	656:659	arg1	G-layers					667:674	bast fiber G-layers	656:674	bast fiber G-layers	656:674	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	4	31	theme	Statistical	519:529	arg1	analysis					531:538	Statistical analysis	519:538	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA)	519:632	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	3	32	theme	opposite	427:434	arg1	side					436:439	the tension and opposite side	411:439	side	436:439	Raman spectral profiles were acquired for xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions.					
36979370	3	33	theme	xylem	391:395	arg1	G-layers					397:404	xylem G-layers	391:404	xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions	391:516	Raman spectral profiles were acquired for xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions.					
36979370	0	34	theme	Bast	36:39	arg1	Fiber					41:45	Bast Fiber and Xylem Cell Walls	36:66	Fiber	41:45	Comparative Analysis of G-Layers in Bast Fiber and Xylem Cell Walls in Flax Using Raman Spectroscopy.					
36979370	6	35	theme	G-layers	878:885	arg1	separation					864:873	separation	864:873	separation of G-layers from tension/opposite-wood sides	864:918	PCA did not allow separation of G-layers from tension/opposite-wood sides.					
36979370	6	36	from	sides	914:918	arg1	separation					864:873	separation	864:873	separation of G-layers from tension/opposite-wood sides	864:918	PCA did not allow separation of G-layers from tension/opposite-wood sides.					
36979370	0	37	from	Walls	62:66	arg1	Flax					71:74	Flax	71:74	Flax	71:74	Comparative Analysis of G-Layers in Bast Fiber and Xylem Cell Walls in Flax Using Raman Spectroscopy.					
36979370	3	38	theme	non-tilted	496:505	arg1	conditions					507:516	non-tilted conditions	496:516	non-tilted conditions	496:516	Raman spectral profiles were acquired for xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions.					
36979370	8	39	theme	xylem	1136:1140	arg1	G-layers					1142:1149	xylem G-layers	1136:1149	xylem G-layers	1136:1149	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	39	theme	xylem	1136:1140	arg1	fibers					1116:1121	bast fibers G-layers and xylem G-layers	1111:1149	bast fibers G-layers and xylem G-layers	1111:1149	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	40	dep	ontogenetical	1254:1266	arg1	parameters					1293:1302	parameters	1293:1302	parameters	1293:1302	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	41	dep	fibers	1116:1121	arg1	G-layers					1123:1130	G-layers	1123:1130	G-layers	1123:1130	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	41	dep	fibers	1116:1121	arg1	G-layers					1142:1149	xylem G-layers	1136:1149	xylem G-layers	1136:1149	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	41	dep	fibers	1116:1121	arg1	fibers					1116:1121	bast fibers G-layers and xylem G-layers	1111:1149	bast fibers G-layers and xylem G-layers	1111:1149	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	42	theme	layer	1228:1232	arg1	category					1234:1241	a different cell wall layer category	1206:1241	a different cell wall layer category based upon ontogenetical and chemical composition parameters	1206:1302	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	3	43	theme	tension	415:421	arg1	side					436:439	the tension and opposite side	411:439	side	436:439	Raman spectral profiles were acquired for xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions.					
36979370	3	44	from	G-layer	461:467	arg1	G-layers					397:404	xylem G-layers	391:404	xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions	391:516	Raman spectral profiles were acquired for xylem G-layers from the tension and opposite side as well as from the G-layer of bast fibers grown under non-tilted conditions.					
36979370	7	45	theme	xylem	951:955	arg1	G-layers					957:964	xylem G-layers	951:964	xylem G-layers	951:964	In contrast, the two types of xylem G-layers could be incompletely discriminated through PLS-DA.					
36979370	8	46	theme	wall	1223:1226	arg1	category					1234:1241	a different cell wall layer category	1206:1241	a different cell wall layer category based upon ontogenetical and chemical composition parameters	1206:1302	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	1	47	theme	xylem	186:190	arg1	walls					197:201	xylem cell walls	186:201	xylem cell walls of tilted flax plants	186:223	In a response to gravitropic stress, G-layers (gelatinous layers) were deposited in xylem cell walls of tilted flax plants.					
36979370	7	48	theme	G-layers	957:964	arg1	types					942:946	the two types	934:946	the two types of xylem G-layers	934:964	In contrast, the two types of xylem G-layers could be incompletely discriminated through PLS-DA.					
36979370	2	49	located	observed	309:316	arg2	G-layers					226:233	G-layers	226:233	G-layers	226:233	G-layers were produced in both tension wood (upper side) as expected but were also observed in opposite wood (lower side).					
36979370	2	49	located	observed	309:316	arg1	wood					330:333	opposite wood	321:333	opposite wood (lower side)	321:346	G-layers were produced in both tension wood (upper side) as expected but were also observed in opposite wood (lower side).					
36979370	4	50	theme	least	590:594	arg1	analysis					616:623	partial least square-discriminant analysis	582:623	partial least square-discriminant analysis (PLS-DA)	582:632	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	4	50	theme	least	590:594	arg1	PLS-DA					626:631	PLS-DA	626:631	PLS-DA	626:631	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	1	51	theme	cell	192:195	arg1	walls					197:201	xylem cell walls	186:201	xylem cell walls of tilted flax plants	186:223	In a response to gravitropic stress, G-layers (gelatinous layers) were deposited in xylem cell walls of tilted flax plants.					
36979370	0	52	theme	Cell	57:60	arg1	Walls					62:66	Bast Fiber and Xylem Cell Walls	36:66	Walls	62:66	Comparative Analysis of G-Layers in Bast Fiber and Xylem Cell Walls in Flax Using Raman Spectroscopy.					
36979370	2	53	dep	wood	330:333	arg1	lower					336:340	lower	336:340	lower	336:340	G-layers were produced in both tension wood (upper side) as expected but were also observed in opposite wood (lower side).					
36979370	4	54	theme	square-discriminant	596:614	arg1	analysis					616:623	partial least square-discriminant analysis	582:623	partial least square-discriminant analysis (PLS-DA)	582:632	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	4	54	theme	square-discriminant	596:614	arg1	PLS-DA					626:631	PLS-DA	626:631	PLS-DA	626:631	Statistical analysis by principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) clearly distinguished bast fiber G-layers from xylem G-layers.					
36979370	0	55	theme	Xylem	51:55	arg1	Walls					62:66	Bast Fiber and Xylem Cell Walls	36:66	Walls	62:66	Comparative Analysis of G-Layers in Bast Fiber and Xylem Cell Walls in Flax Using Raman Spectroscopy.					
36979370	1	56	theme	gravitropic	119:129	arg1	stress					131:136	gravitropic stress	119:136	gravitropic stress	119:136	In a response to gravitropic stress, G-layers (gelatinous layers) were deposited in xylem cell walls of tilted flax plants.					
36979370	8	57	theme	spatial	1086:1092	arg1	distribution					1094:1105	polymer spatial distribution	1078:1105	polymer spatial distribution	1078:1105	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36979370	8	57	theme	spatial	1086:1092	arg1	architecture					1064:1075	the architecture	1060:1075	the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers	1060:1149	Overall, the results suggested that while the architecture (polymer spatial distribution) of bast fibers G-layers and xylem G-layers are similar, they should be considered as belonging to a different cell wall layer category based upon ontogenetical and chemical composition parameters.					
36142684	5	0	theme	different	846:854	arg1	substrates					856:865	different substrates	846:865	different substrates	846:865	Enrichment cultures facilitated growth of similar, but not identical cellulose-decomposing bacteria from different substrates.					
36142684	3	1	theme	effective	533:541	arg1	decomposition					543:555	rapid and effective decomposition	523:555	rapid and effective decomposition of plant biomass	523:572	Thus, using microbial consortia can be a solution to rapid and effective decomposition of plant biomass.					
36142684	5	2	from	growth	773:778	arg1	substrates					856:865	different substrates	846:865	different substrates	846:865	Enrichment cultures facilitated growth of similar, but not identical cellulose-decomposing bacteria from different substrates.					
36142684	6	3	from	sawdust	1078:1084	arg1	substrates-Verrucomicrobiota					1000:1027	different substrates-Verrucomicrobiota	990:1027	different substrates-Verrucomicrobiota	990:1027	Major components in all consortia were from Proteobacteria, Actinobacteriota and Bacteroidota, but some were specific for different substrates-Verrucomicrobiota and Myxococcota from straw, Planctomycetota from sawdust and Firmicutes from leaf litter.					
36142684	4	4	theme	enrichment	622:631	arg1	cultures					633:640	enrichment cultures	622:640	enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter	622:738	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	7	5	theme	additional	1222:1231	arg1	activities					1243:1252	additional metabolic activities	1222:1252	additional metabolic activities	1222:1252	While most members of the consortia were involved in the lignocellulose degradation, some demonstrated additional metabolic activities.					
36142684	9	6	from	substrates	1575:1584	arg1	consortia					1536:1544	reproducible cellulolytic consortia	1510:1544	reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components	1510:1671	Our findings show that enrichment cultures can provide reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components.					
36142684	4	7	theme	composting	647:656	arg1	straw					697:701	composting natural lignocellulosic substrates-oat straw	647:701	composting natural lignocellulosic substrates-oat straw	647:701	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	2	8	theme	responsible	355:365	arg1	CAZymes					317:323	the CAZymes	313:323	the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities	313:386	Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat.					
36142684	2	8	theme	responsible	355:365	arg1	enZYmes					346:352	Carbohydrate-Active enZYmes	326:352	Carbohydrate-Active enZYmes	326:352	Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat.					
36142684	2	8	theme	responsible	355:365	arg1	diverse					397:403	diverse	397:403	diverse	397:403	Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat.					
36142684	6	9	theme	different	990:998	arg1	substrates-Verrucomicrobiota					1000:1027	different substrates-Verrucomicrobiota	990:1027	different substrates-Verrucomicrobiota	990:1027	Major components in all consortia were from Proteobacteria, Actinobacteriota and Bacteroidota, but some were specific for different substrates-Verrucomicrobiota and Myxococcota from straw, Planctomycetota from sawdust and Firmicutes from leaf litter.					
36142684	5	10	theme	bacteria	832:839	arg1	growth					773:778	growth	773:778	growth of similar, but not identical cellulose-decomposing bacteria from different substrates	773:865	Enrichment cultures facilitated growth of similar, but not identical cellulose-decomposing bacteria from different substrates.					
36142684	4	11	from	litter	733:738	arg1	cultures					633:640	enrichment cultures	622:640	enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter	622:738	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	6	12	theme	leaf	1106:1109	arg1	litter					1111:1116	leaf litter	1106:1116	leaf litter	1106:1116	Major components in all consortia were from Proteobacteria, Actinobacteriota and Bacteroidota, but some were specific for different substrates-Verrucomicrobiota and Myxococcota from straw, Planctomycetota from sawdust and Firmicutes from leaf litter.					
36142684	5	13	theme	identical	800:808	arg1	bacteria					832:839	similar, but not identical cellulose-decomposing bacteria	783:839	similar, but not identical cellulose-decomposing bacteria from different substrates	783:865	Enrichment cultures facilitated growth of similar, but not identical cellulose-decomposing bacteria from different substrates.					
36142684	8	14	theme	associated	1430:1439	arg1	ABC-transporters					1359:1374	ABC-transporters	1359:1374	ABC-transporters	1359:1374	Consortia did not differ in the composition of CAZymes genes, but rather in axillary functions, such as ABC-transporters and two-component systems, usually taxon-specific and associated with CAZymes.					
36142684	6	15	from	litter	1111:1116	arg1	substrates-Verrucomicrobiota					1000:1027	different substrates-Verrucomicrobiota	990:1027	different substrates-Verrucomicrobiota	990:1027	Major components in all consortia were from Proteobacteria, Actinobacteriota and Bacteroidota, but some were specific for different substrates-Verrucomicrobiota and Myxococcota from straw, Planctomycetota from sawdust and Firmicutes from leaf litter.					
36142684	9	16	theme	microbial	1630:1638	arg1	relations					1640:1648	tight microbial relations	1624:1648	tight microbial relations	1624:1648	Our findings show that enrichment cultures can provide reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components.					
36142684	3	17	theme	biomass	566:572	arg1	decomposition					543:555	rapid and effective decomposition	523:555	rapid and effective decomposition of plant biomass	523:572	Thus, using microbial consortia can be a solution to rapid and effective decomposition of plant biomass.					
36142684	8	18	theme	two-component	1380:1392	arg1	systems					1394:1400	two-component systems	1380:1400	two-component systems	1380:1400	Consortia did not differ in the composition of CAZymes genes, but rather in axillary functions, such as ABC-transporters and two-component systems, usually taxon-specific and associated with CAZymes.					
36142684	4	19	theme	birch	722:726	arg1	litter					733:738	birch leaf litter	722:738	birch leaf litter	722:738	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	5	20	theme	cellulose-decomposing	810:830	arg1	bacteria					832:839	similar, but not identical cellulose-decomposing bacteria	783:839	similar, but not identical cellulose-decomposing bacteria from different substrates	783:865	Enrichment cultures facilitated growth of similar, but not identical cellulose-decomposing bacteria from different substrates.					
36142684	7	21	theme	metabolic	1233:1241	arg1	activities					1243:1252	additional metabolic activities	1222:1252	additional metabolic activities	1222:1252	While most members of the consortia were involved in the lignocellulose degradation, some demonstrated additional metabolic activities.					
36142684	9	22	theme	various	1551:1557	arg1	substrates					1575:1584	various lignocellulosic substrates	1551:1584	various lignocellulosic substrates	1551:1584	Our findings show that enrichment cultures can provide reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components.					
36142684	9	23	theme	cellulolytic	1523:1534	arg1	consortia					1536:1544	reproducible cellulolytic consortia	1510:1544	reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components	1510:1671	Our findings show that enrichment cultures can provide reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components.					
36142684	2	24	theme	different	435:443	arg1	bacteria					445:452	different bacteria	435:452	different bacteria in one habitat	435:467	Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat.					
36142684	4	25	from	straw	697:701	arg1	cultures					633:640	enrichment cultures	622:640	enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter	622:738	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	1	26	theme	Recycling	97:105	arg1	matter					113:118	Recycling plant matter	97:118	Recycling plant matter	97:118	Recycling plant matter is one of the challenges facing humanity today and depends on efficient lignocellulose degradation.					
36142684	6	27	from	straw	1050:1054	arg1	substrates-Verrucomicrobiota					1000:1027	different substrates-Verrucomicrobiota	990:1027	different substrates-Verrucomicrobiota	990:1027	Major components in all consortia were from Proteobacteria, Actinobacteriota and Bacteroidota, but some were specific for different substrates-Verrucomicrobiota and Myxococcota from straw, Planctomycetota from sawdust and Firmicutes from leaf litter.					
36142684	1	28	theme	efficient	182:190	arg1	degradation					207:217	efficient lignocellulose degradation	182:217	efficient lignocellulose degradation	182:217	Recycling plant matter is one of the challenges facing humanity today and depends on efficient lignocellulose degradation.					
36142684	6	29	theme	Major	868:872	arg1	components					874:883	Major components	868:883	Major components in all consortia	868:900	Major components in all consortia were from Proteobacteria, Actinobacteriota and Bacteroidota, but some were specific for different substrates-Verrucomicrobiota and Myxococcota from straw, Planctomycetota from sawdust and Firmicutes from leaf litter.					
36142684	1	30	theme	plant	107:111	arg1	matter					113:118	Recycling plant matter	97:118	Recycling plant matter	97:118	Recycling plant matter is one of the challenges facing humanity today and depends on efficient lignocellulose degradation.					
36142684	1	31	theme	lignocellulose	192:205	arg1	degradation					207:217	efficient lignocellulose degradation	182:217	efficient lignocellulose degradation	182:217	Recycling plant matter is one of the challenges facing humanity today and depends on efficient lignocellulose degradation.					
36142684	0	32	theme	Stable	17:22	arg1	Consortia					37:45	Stable Cellulolytic Consortia	17:45	Stable Cellulolytic Consortia	17:45	The Structure of Stable Cellulolytic Consortia Isolated from Natural Lignocellulosic Substrates.					
36142684	8	33	theme	CAZymes	1302:1308	arg1	genes					1310:1314	CAZymes genes	1302:1314	CAZymes genes	1302:1314	Consortia did not differ in the composition of CAZymes genes, but rather in axillary functions, such as ABC-transporters and two-component systems, usually taxon-specific and associated with CAZymes.					
36142684	2	34	from	substrates	265:274	arg1	strains					244:250	many bacterial strains	229:250	many bacterial strains from natural substrates	229:274	Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat.					
36142684	4	35	theme	pine	704:707	arg1	sawdust					709:715	pine sawdust	704:715	pine sawdust	704:715	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	4	36	attach	isolated	608:615	arg2	consortia					593:601	Four cellulolytic consortia	575:601	Four cellulolytic consortia	575:601	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	4	36	attach	isolated	608:615	arg1	cultures					633:640	enrichment cultures	622:640	enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter	622:738	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	2	37	theme	Carbohydrate-Active	326:344	arg1	CAZymes					317:323	the CAZymes	313:323	the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities	313:386	Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat.					
36142684	2	37	theme	Carbohydrate-Active	326:344	arg1	enZYmes					346:352	Carbohydrate-Active enZYmes	326:352	Carbohydrate-Active enZYmes	326:352	Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat.					
36142684	4	38	from	sawdust	709:715	arg1	cultures					633:640	enrichment cultures	622:640	enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter	622:738	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	0	39	theme	Consortia	37:45	arg1	Structure					4:12	The Structure	0:12	The Structure of Stable Cellulolytic Consortia	0:45	The Structure of Stable Cellulolytic Consortia Isolated from Natural Lignocellulosic Substrates.					
36142684	5	40	theme	similar	783:789	arg1	bacteria					832:839	similar, but not identical cellulose-decomposing bacteria	783:839	similar, but not identical cellulose-decomposing bacteria from different substrates	783:865	Enrichment cultures facilitated growth of similar, but not identical cellulose-decomposing bacteria from different substrates.					
36142684	2	41	theme	natural	257:263	arg1	substrates					265:274	natural substrates	257:274	natural substrates	257:274	Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat.					
36142684	4	42	theme	leaf	728:731	arg1	litter					733:738	birch leaf litter	722:738	birch leaf litter	722:738	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	8	43	theme	taxon-specific	1411:1424	arg1	ABC-transporters					1359:1374	ABC-transporters	1359:1374	ABC-transporters	1359:1374	Consortia did not differ in the composition of CAZymes genes, but rather in axillary functions, such as ABC-transporters and two-component systems, usually taxon-specific and associated with CAZymes.					
36142684	0	44	theme	Cellulolytic	24:35	arg1	Consortia					37:45	Stable Cellulolytic Consortia	17:45	Stable Cellulolytic Consortia	17:45	The Structure of Stable Cellulolytic Consortia Isolated from Natural Lignocellulosic Substrates.					
36142684	7	45	theme	lignocellulose	1176:1189	arg1	degradation					1191:1201	the lignocellulose degradation	1172:1201	the lignocellulose degradation	1172:1201	While most members of the consortia were involved in the lignocellulose degradation, some demonstrated additional metabolic activities.					
36142684	6	46	from	components	874:883	arg1	consortia					892:900	all consortia	888:900	all consortia	888:900	Major components in all consortia were from Proteobacteria, Actinobacteriota and Bacteroidota, but some were specific for different substrates-Verrucomicrobiota and Myxococcota from straw, Planctomycetota from sawdust and Firmicutes from leaf litter.					
36142684	9	47	theme	reproducible	1510:1521	arg1	consortia					1536:1544	reproducible cellulolytic consortia	1510:1544	reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components	1510:1671	Our findings show that enrichment cultures can provide reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components.					
36142684	8	48	theme	axillary	1331:1338	arg1	functions					1340:1348	axillary functions	1331:1348	axillary functions	1331:1348	Consortia did not differ in the composition of CAZymes genes, but rather in axillary functions, such as ABC-transporters and two-component systems, usually taxon-specific and associated with CAZymes.					
36142684	8	48	theme	axillary	1331:1338	arg1	ABC-transporters					1359:1374	ABC-transporters	1359:1374	ABC-transporters	1359:1374	Consortia did not differ in the composition of CAZymes genes, but rather in axillary functions, such as ABC-transporters and two-component systems, usually taxon-specific and associated with CAZymes.					
36142684	8	48	theme	axillary	1331:1338	arg1	systems					1394:1400	two-component systems	1380:1400	two-component systems	1380:1400	Consortia did not differ in the composition of CAZymes genes, but rather in axillary functions, such as ABC-transporters and two-component systems, usually taxon-specific and associated with CAZymes.					
36142684	9	49	theme	enrichment	1478:1487	arg1	cultures					1489:1496	enrichment cultures	1478:1496	enrichment cultures	1478:1496	Our findings show that enrichment cultures can provide reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components.					
36142684	7	50	theme	most	1125:1128	arg1	members					1130:1136	most members	1125:1136	most members of the consortia	1125:1153	While most members of the consortia were involved in the lignocellulose degradation, some demonstrated additional metabolic activities.					
36142684	3	51	theme	rapid	523:527	arg1	decomposition					543:555	rapid and effective decomposition	523:555	rapid and effective decomposition of plant biomass	523:572	Thus, using microbial consortia can be a solution to rapid and effective decomposition of plant biomass.					
36142684	4	52	theme	cellulolytic	580:591	arg1	consortia					593:601	Four cellulolytic consortia	575:601	Four cellulolytic consortia	575:601	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	5	53	theme	Enrichment	741:750	arg1	cultures					752:759	Enrichment cultures	741:759	Enrichment cultures	741:759	Enrichment cultures facilitated growth of similar, but not identical cellulose-decomposing bacteria from different substrates.					
36142684	2	54	theme	many	229:232	arg1	strains					244:250	many bacterial strains	229:250	many bacterial strains from natural substrates	229:274	Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat.					
36142684	2	55	theme	bacterial	234:242	arg1	strains					244:250	many bacterial strains	229:250	many bacterial strains from natural substrates	229:274	Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat.					
36142684	2	56	from	bacteria	445:452	arg1	habitat					461:467	one habitat	457:467	one habitat	457:467	Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat.					
36142684	7	57	theme	consortia	1145:1153	arg1	members					1130:1136	most members	1125:1136	most members of the consortia	1125:1153	While most members of the consortia were involved in the lignocellulose degradation, some demonstrated additional metabolic activities.					
36142684	5	58	from	substrates	856:865	arg1	bacteria					832:839	similar, but not identical cellulose-decomposing bacteria	783:839	similar, but not identical cellulose-decomposing bacteria from different substrates	783:865	Enrichment cultures facilitated growth of similar, but not identical cellulose-decomposing bacteria from different substrates.					
36142684	5	58	from	substrates	856:865	arg1	growth					773:778	growth	773:778	growth of similar, but not identical cellulose-decomposing bacteria from different substrates	773:865	Enrichment cultures facilitated growth of similar, but not identical cellulose-decomposing bacteria from different substrates.					
36142684	9	59	theme	tight	1624:1628	arg1	relations					1640:1648	tight microbial relations	1624:1648	tight microbial relations	1624:1648	Our findings show that enrichment cultures can provide reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components.					
36142684	4	60	theme	lignocellulosic	666:680	arg1	straw					697:701	composting natural lignocellulosic substrates-oat straw	647:701	composting natural lignocellulosic substrates-oat straw	647:701	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	8	61	theme	genes	1310:1314	arg1	composition					1287:1297	the composition	1283:1297	the composition of CAZymes genes	1283:1314	Consortia did not differ in the composition of CAZymes genes, but rather in axillary functions, such as ABC-transporters and two-component systems, usually taxon-specific and associated with CAZymes.					
36142684	2	62	theme	cellulolytic	288:299	arg1	activities					301:310	cellulolytic activities	288:310	cellulolytic activities	288:310	Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat.					
36142684	1	63	theme	challenges	134:143	arg1	one					123:125	one	123:125	one	123:125	Recycling plant matter is one of the challenges facing humanity today and depends on efficient lignocellulose degradation.					
36142684	1	63	theme	challenges	134:143	arg1	challenges					134:143	the challenges	130:143	the challenges facing humanity today	130:165	Recycling plant matter is one of the challenges facing humanity today and depends on efficient lignocellulose degradation.					
36142684	4	64	theme	natural	658:664	arg1	straw					697:701	composting natural lignocellulosic substrates-oat straw	647:701	composting natural lignocellulosic substrates-oat straw	647:701	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
36142684	0	65	theme	Lignocellulosic	69:83	arg1	Substrates					85:94	Natural Lignocellulosic Substrates	61:94	Natural Lignocellulosic Substrates	61:94	The Structure of Stable Cellulolytic Consortia Isolated from Natural Lignocellulosic Substrates.					
36142684	1	66	theme	humanity	152:159	arg1	today					161:165	humanity today	152:165	humanity today	152:165	Recycling plant matter is one of the challenges facing humanity today and depends on efficient lignocellulose degradation.					
36142684	3	67	theme	microbial	482:490	arg1	consortia					492:500	microbial consortia	482:500	microbial consortia	482:500	Thus, using microbial consortia can be a solution to rapid and effective decomposition of plant biomass.					
36142684	9	68	theme	lignocellulosic	1559:1573	arg1	substrates					1575:1584	various lignocellulosic substrates	1551:1584	various lignocellulosic substrates	1551:1584	Our findings show that enrichment cultures can provide reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components.					
36142684	0	69	theme	Natural	61:67	arg1	Substrates					85:94	Natural Lignocellulosic Substrates	61:94	Natural Lignocellulosic Substrates	61:94	The Structure of Stable Cellulolytic Consortia Isolated from Natural Lignocellulosic Substrates.					
36142684	9	70	theme	substrates	1575:1584	arg1	stability					1591:1599	the stability	1587:1599	the stability	1587:1599	Our findings show that enrichment cultures can provide reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components.					
36142684	3	71	theme	plant	560:564	arg1	biomass					566:572	plant biomass	560:572	plant biomass	560:572	Thus, using microbial consortia can be a solution to rapid and effective decomposition of plant biomass.					
36142684	4	72	theme	substrates-oat	682:695	arg1	straw					697:701	composting natural lignocellulosic substrates-oat straw	647:701	composting natural lignocellulosic substrates-oat straw	647:701	Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates-oat straw, pine sawdust, and birch leaf litter.					
35807944	8	0	theme	chain	1000:1004	arg1	SCFAs					1019:1023	SCFAs	1019:1023	SCFAs	1019:1023	Notably, the low-dose sucrose restored microbial dysfunction and enhanced the production of short chain fatty acids (SCFAs).					
35807944	8	0	theme	chain	1000:1004	arg1	acids					1012:1016	short chain fatty acids	994:1016	short chain fatty acids (SCFAs)	994:1024	Notably, the low-dose sucrose restored microbial dysfunction and enhanced the production of short chain fatty acids (SCFAs).					
35807944	6	1	from	contrast	744:751	arg1	exacerbated					785:795	exacerbated	785:795	exacerbated	785:795	In contrast, high-dose sucrose intervention exacerbated colitis.					
35807944	9	2	theme	LOW	1163:1165	arg1	group					1167:1171	the LOW group	1159:1171	the LOW group	1159:1171	Specifically, the abundance of SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia were increased significantly in the LOW group.					
35807944	0	3	theme	PPAR-γ/MAPK/NF-κB	80:96	arg1	Pathway					98:104	PPAR-γ/MAPK/NF-κB Pathway	80:104	PPAR-γ/MAPK/NF-κB Pathway	80:104	Different Dose of Sucrose Consumption Divergently Influences Gut Microbiota and PPAR-γ/MAPK/NF-κB Pathway in DSS-Induced Colitis Mice.					
35807944	4	4	theme	sucrose	500:506	arg1	doses					491:495	three doses	485:495	three doses of sucrose	485:506	Hence, our study aimed to elucidate the potential role of three doses of sucrose on DSS-induced colitis in C57BL/6 mice and the underlying mechanisms.					
35807944	11	5	theme	different	1373:1381	arg1	doses					1383:1387	different doses	1373:1387	different doses	1373:1387	Together, our study demonstrates the differential effects of sucrose on colitis at different doses, providing a scientific basis for measuring and modifying the safe intake level of sugar and providing favorable evidence for implementing sugar reduction policies.					
35807944	10	6	theme	LOW	1236:1238	arg1	group					1240:1244	the LOW group	1232:1244	the LOW group	1232:1244	Consistently, PPAR-γ, activated by SCFAs, was elevated in the LOW group, thereby inhibiting the MAPK/NF-κB pathway.					
35807944	4	7	theme	underlying	555:564	arg1	mechanisms					566:575	the underlying mechanisms	551:575	the underlying mechanisms	551:575	Hence, our study aimed to elucidate the potential role of three doses of sucrose on DSS-induced colitis in C57BL/6 mice and the underlying mechanisms.					
35807944	0	8	theme	DSS-Induced	109:119	arg1	Colitis					121:127	DSS-Induced Colitis	109:127	DSS-Induced Colitis Mice	109:132	Different Dose of Sucrose Consumption Divergently Influences Gut Microbiota and PPAR-γ/MAPK/NF-κB Pathway in DSS-Induced Colitis Mice.					
35807944	8	9	theme	acids	1012:1016	arg1	production					980:989	the production	976:989	the production of short chain fatty acids (SCFAs)	976:1024	Notably, the low-dose sucrose restored microbial dysfunction and enhanced the production of short chain fatty acids (SCFAs).					
35807944	10	10	theme	MAPK/NF-κB	1270:1279	arg1	pathway					1281:1287	the MAPK/NF-κB pathway	1266:1287	the MAPK/NF-κB pathway	1266:1287	Consistently, PPAR-γ, activated by SCFAs, was elevated in the LOW group, thereby inhibiting the MAPK/NF-κB pathway.					
35807944	4	11	theme	potential	467:475	arg1	role					477:480	the potential role	463:480	the potential role of three doses of sucrose on DSS-induced colitis in C57BL/6 mice	463:545	Hence, our study aimed to elucidate the potential role of three doses of sucrose on DSS-induced colitis in C57BL/6 mice and the underlying mechanisms.					
35807944	0	12	theme	Different	0:8	arg1	Dose					10:13	Different Dose	0:13	Different Dose of Sucrose Consumption	0:36	Different Dose of Sucrose Consumption Divergently Influences Gut Microbiota and PPAR-γ/MAPK/NF-κB Pathway in DSS-Induced Colitis Mice.					
35807944	11	13	theme	intake	1456:1461	arg1	level					1463:1467	the safe intake level	1447:1467	the safe intake level of sugar	1447:1476	Together, our study demonstrates the differential effects of sucrose on colitis at different doses, providing a scientific basis for measuring and modifying the safe intake level of sugar and providing favorable evidence for implementing sugar reduction policies.					
35807944	4	14	from	mechanisms	566:575	arg1	colitis					523:529	DSS-induced colitis	511:529	DSS-induced colitis in C57BL/6 mice	511:545	Hence, our study aimed to elucidate the potential role of three doses of sucrose on DSS-induced colitis in C57BL/6 mice and the underlying mechanisms.					
35807944	5	15	theme	sucrose	611:617	arg1	intervention					619:630	low-dose sucrose intervention	602:630	low-dose sucrose intervention	602:630	The results showed that low-dose sucrose intervention alleviated colitis in mice, reducing the expression of inflammatory cytokines and repairing mucosal damages.					
35807944	5	16	theme	low-dose	602:609	arg1	intervention					619:630	low-dose sucrose intervention	602:630	low-dose sucrose intervention	602:630	The results showed that low-dose sucrose intervention alleviated colitis in mice, reducing the expression of inflammatory cytokines and repairing mucosal damages.					
35807944	0	17	theme	Colitis	121:127	arg1	Mice					129:132	DSS-Induced Colitis Mice	109:132	DSS-Induced Colitis Mice	109:132	Different Dose of Sucrose Consumption Divergently Influences Gut Microbiota and PPAR-γ/MAPK/NF-κB Pathway in DSS-Induced Colitis Mice.					
35807944	4	18	from	role	477:480	arg1	colitis					523:529	DSS-induced colitis	511:529	DSS-induced colitis in C57BL/6 mice	511:545	Hence, our study aimed to elucidate the potential role of three doses of sucrose on DSS-induced colitis in C57BL/6 mice and the underlying mechanisms.					
35807944	2	19	theme	daily	274:278	arg1	diet					280:283	our daily diet	270:283	our daily diet	270:283	Sucrose, as the widely consumed ingredient in our daily diet, has been reported a relation to gastrointestinal diseases.					
35807944	7	20	theme	gut	874:876	arg1	composition					889:899	gut microbiota composition	874:899	gut microbiota composition	874:899	Furthermore, three doses of sucrose administration markedly altered gut microbiota composition.					
35807944	11	21	theme	scientific	1402:1411	arg1	basis					1413:1417	a scientific basis	1400:1417	a scientific basis for measuring and modifying the safe intake level of sugar and providing favorable evidence for implementing sugar reduction policies	1400:1551	Together, our study demonstrates the differential effects of sucrose on colitis at different doses, providing a scientific basis for measuring and modifying the safe intake level of sugar and providing favorable evidence for implementing sugar reduction policies.					
35807944	11	22	theme	sugar	1472:1476	arg1	level					1463:1467	the safe intake level	1447:1467	the safe intake level of sugar	1447:1476	Together, our study demonstrates the differential effects of sucrose on colitis at different doses, providing a scientific basis for measuring and modifying the safe intake level of sugar and providing favorable evidence for implementing sugar reduction policies.					
35807944	7	23	theme	microbiota	878:887	arg1	composition					889:899	gut microbiota composition	874:899	gut microbiota composition	874:899	Furthermore, three doses of sucrose administration markedly altered gut microbiota composition.					
35807944	3	24	theme	inflammatory	377:388	arg1	disease					396:402	inflammatory bowel disease	377:402	inflammatory bowel disease	377:402	However, the role of sucrose in inflammatory bowel disease remains controversial.					
35807944	11	25	theme	differential	1327:1338	arg1	effects					1340:1346	the differential effects	1323:1346	the differential effects of sucrose on colitis	1323:1368	Together, our study demonstrates the differential effects of sucrose on colitis at different doses, providing a scientific basis for measuring and modifying the safe intake level of sugar and providing favorable evidence for implementing sugar reduction policies.					
35807944	2	26	from	ingredient	256:265	arg1	diet					280:283	our daily diet	270:283	our daily diet	270:283	Sucrose, as the widely consumed ingredient in our daily diet, has been reported a relation to gastrointestinal diseases.					
35807944	0	27	theme	Sucrose	18:24	arg1	Consumption					26:36	Sucrose Consumption	18:36	Sucrose Consumption	18:36	Different Dose of Sucrose Consumption Divergently Influences Gut Microbiota and PPAR-γ/MAPK/NF-κB Pathway in DSS-Induced Colitis Mice.					
35807944	4	28	theme	C57BL/6	534:540	arg1	mice					542:545	C57BL/6 mice	534:545	C57BL/6 mice	534:545	Hence, our study aimed to elucidate the potential role of three doses of sucrose on DSS-induced colitis in C57BL/6 mice and the underlying mechanisms.					
35807944	5	29	theme	cytokines	700:708	arg1	expression					673:682	the expression	669:682	the expression of inflammatory cytokines	669:708	The results showed that low-dose sucrose intervention alleviated colitis in mice, reducing the expression of inflammatory cytokines and repairing mucosal damages.					
35807944	11	30	theme	favorable	1492:1500	arg1	evidence					1502:1509	favorable evidence	1492:1509	favorable evidence for implementing sugar reduction policies	1492:1551	Together, our study demonstrates the differential effects of sucrose on colitis at different doses, providing a scientific basis for measuring and modifying the safe intake level of sugar and providing favorable evidence for implementing sugar reduction policies.					
35807944	3	31	theme	sucrose	366:372	arg1	role					358:361	the role	354:361	the role of sucrose in inflammatory bowel disease	354:402	However, the role of sucrose in inflammatory bowel disease remains controversial.					
35807944	11	32	from	effects	1340:1346	arg1	colitis					1362:1368	colitis	1362:1368	colitis	1362:1368	Together, our study demonstrates the differential effects of sucrose on colitis at different doses, providing a scientific basis for measuring and modifying the safe intake level of sugar and providing favorable evidence for implementing sugar reduction policies.					
35807944	8	33	theme	microbial	941:949	arg1	dysfunction					951:961	microbial dysfunction	941:961	microbial dysfunction	941:961	Notably, the low-dose sucrose restored microbial dysfunction and enhanced the production of short chain fatty acids (SCFAs).					
35807944	7	34	theme	sucrose	834:840	arg1	administration					842:855	sucrose administration	834:855	sucrose administration	834:855	Furthermore, three doses of sucrose administration markedly altered gut microbiota composition.					
35807944	6	35	from	exacerbated	785:795	arg1	contrast					744:751	contrast	744:751	contrast	744:751	In contrast, high-dose sucrose intervention exacerbated colitis.					
35807944	5	36	dep	alleviated	632:641	arg1	repairing					714:722	repairing	714:722	repairing mucosal damages	714:738	The results showed that low-dose sucrose intervention alleviated colitis in mice, reducing the expression of inflammatory cytokines and repairing mucosal damages.					
35807944	5	36	dep	alleviated	632:641	arg1	reducing					660:667	reducing	660:667	reducing the expression of inflammatory cytokines	660:708	The results showed that low-dose sucrose intervention alleviated colitis in mice, reducing the expression of inflammatory cytokines and repairing mucosal damages.					
35807944	11	37	theme	reduction	1534:1542	arg1	policies					1544:1551	sugar reduction policies	1528:1551	sugar reduction policies	1528:1551	Together, our study demonstrates the differential effects of sucrose on colitis at different doses, providing a scientific basis for measuring and modifying the safe intake level of sugar and providing favorable evidence for implementing sugar reduction policies.					
35807944	7	38	theme	administration	842:855	arg1	doses					825:829	three doses	819:829	three doses of sucrose administration	819:855	Furthermore, three doses of sucrose administration markedly altered gut microbiota composition.					
35807944	0	39	theme	Consumption	26:36	arg1	Dose					10:13	Different Dose	0:13	Different Dose of Sucrose Consumption	0:36	Different Dose of Sucrose Consumption Divergently Influences Gut Microbiota and PPAR-γ/MAPK/NF-κB Pathway in DSS-Induced Colitis Mice.					
35807944	4	40	theme	DSS-induced	511:521	arg1	colitis					523:529	DSS-induced colitis	511:529	DSS-induced colitis in C57BL/6 mice	511:545	Hence, our study aimed to elucidate the potential role of three doses of sucrose on DSS-induced colitis in C57BL/6 mice and the underlying mechanisms.					
35807944	4	41	from	colitis	523:529	arg1	mice					542:545	C57BL/6 mice	534:545	C57BL/6 mice	534:545	Hence, our study aimed to elucidate the potential role of three doses of sucrose on DSS-induced colitis in C57BL/6 mice and the underlying mechanisms.					
35807944	11	42	theme	sucrose	1351:1357	arg1	effects					1340:1346	the differential effects	1323:1346	the differential effects of sucrose on colitis	1323:1368	Together, our study demonstrates the differential effects of sucrose on colitis at different doses, providing a scientific basis for measuring and modifying the safe intake level of sugar and providing favorable evidence for implementing sugar reduction policies.					
35807944	0	43	theme	Gut	61:63	arg1	Microbiota					65:74	Gut Microbiota	61:74	Gut Microbiota	61:74	Different Dose of Sucrose Consumption Divergently Influences Gut Microbiota and PPAR-γ/MAPK/NF-κB Pathway in DSS-Induced Colitis Mice.					
35807944	3	44	from	role	358:361	arg1	disease					396:402	inflammatory bowel disease	377:402	inflammatory bowel disease	377:402	However, the role of sucrose in inflammatory bowel disease remains controversial.					
35807944	6	45	theme	sucrose	764:770	arg1	intervention					772:783	high-dose sucrose intervention	754:783	high-dose sucrose intervention	754:783	In contrast, high-dose sucrose intervention exacerbated colitis.					
35807944	5	46	theme	mucosal	724:730	arg1	damages					732:738	mucosal damages	724:738	mucosal damages	724:738	The results showed that low-dose sucrose intervention alleviated colitis in mice, reducing the expression of inflammatory cytokines and repairing mucosal damages.					
35807944	11	47	theme	safe	1451:1454	arg1	level					1463:1467	the safe intake level	1447:1467	the safe intake level of sugar	1447:1476	Together, our study demonstrates the differential effects of sucrose on colitis at different doses, providing a scientific basis for measuring and modifying the safe intake level of sugar and providing favorable evidence for implementing sugar reduction policies.					
35807944	6	48	theme	high-dose	754:762	arg1	intervention					772:783	high-dose sucrose intervention	754:783	high-dose sucrose intervention	754:783	In contrast, high-dose sucrose intervention exacerbated colitis.					
35807944	1	49	theme	Sugar	135:139	arg1	reduction					141:149	Sugar reduction	135:149	Sugar reduction	135:149	Sugar reduction and sugar control are advocated and gaining popularity around the world.					
35807944	9	50	dep	bacteria	1074:1081	arg1	bacteria					1074:1081	SCFAs-producing bacteria	1058:1081	SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia	1058:1125	Specifically, the abundance of SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia were increased significantly in the LOW group.					
35807944	9	50	dep	bacteria	1074:1081	arg1	Bacteroides					1099:1109	Bacteroides	1099:1109	Bacteroides	1099:1109	Specifically, the abundance of SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia were increased significantly in the LOW group.					
35807944	9	50	dep	bacteria	1074:1081	arg1	Romboutsia					1116:1125	Romboutsia	1116:1125	Romboutsia	1116:1125	Specifically, the abundance of SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia were increased significantly in the LOW group.					
35807944	9	50	dep	bacteria	1074:1081	arg1	Faecalibaculum					1083:1096	Faecalibaculum	1083:1096	Faecalibaculum	1083:1096	Specifically, the abundance of SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia were increased significantly in the LOW group.					
35807944	2	51	theme	gastrointestinal	318:333	arg1	diseases					335:342	gastrointestinal diseases	318:342	gastrointestinal diseases	318:342	Sucrose, as the widely consumed ingredient in our daily diet, has been reported a relation to gastrointestinal diseases.					
35807944	11	52	theme	sugar	1528:1532	arg1	policies					1544:1551	sugar reduction policies	1528:1551	sugar reduction policies	1528:1551	Together, our study demonstrates the differential effects of sucrose on colitis at different doses, providing a scientific basis for measuring and modifying the safe intake level of sugar and providing favorable evidence for implementing sugar reduction policies.					
35807944	5	53	theme	inflammatory	687:698	arg1	cytokines					700:708	inflammatory cytokines	687:708	inflammatory cytokines	687:708	The results showed that low-dose sucrose intervention alleviated colitis in mice, reducing the expression of inflammatory cytokines and repairing mucosal damages.					
35807944	8	54	theme	short	994:998	arg1	SCFAs					1019:1023	SCFAs	1019:1023	SCFAs	1019:1023	Notably, the low-dose sucrose restored microbial dysfunction and enhanced the production of short chain fatty acids (SCFAs).					
35807944	8	54	theme	short	994:998	arg1	acids					1012:1016	short chain fatty acids	994:1016	short chain fatty acids (SCFAs)	994:1024	Notably, the low-dose sucrose restored microbial dysfunction and enhanced the production of short chain fatty acids (SCFAs).					
35807944	9	55	theme	SCFAs-producing	1058:1072	arg1	bacteria					1074:1081	SCFAs-producing bacteria	1058:1081	SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia	1058:1125	Specifically, the abundance of SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia were increased significantly in the LOW group.					
35807944	9	55	theme	SCFAs-producing	1058:1072	arg1	Bacteroides					1099:1109	Bacteroides	1099:1109	Bacteroides	1099:1109	Specifically, the abundance of SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia were increased significantly in the LOW group.					
35807944	9	55	theme	SCFAs-producing	1058:1072	arg1	Romboutsia					1116:1125	Romboutsia	1116:1125	Romboutsia	1116:1125	Specifically, the abundance of SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia were increased significantly in the LOW group.					
35807944	9	55	theme	SCFAs-producing	1058:1072	arg1	Faecalibaculum					1083:1096	Faecalibaculum	1083:1096	Faecalibaculum	1083:1096	Specifically, the abundance of SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia were increased significantly in the LOW group.					
35807944	8	56	theme	low-dose	915:922	arg1	sucrose					924:930	the low-dose sucrose	911:930	the low-dose sucrose	911:930	Notably, the low-dose sucrose restored microbial dysfunction and enhanced the production of short chain fatty acids (SCFAs).					
35807944	1	57	theme	sugar	155:159	arg1	control					161:167	sugar control	155:167	sugar control	155:167	Sugar reduction and sugar control are advocated and gaining popularity around the world.					
35807944	9	58	theme	bacteria	1074:1081	arg1	abundance					1045:1053	the abundance	1041:1053	the abundance of SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia	1041:1125	Specifically, the abundance of SCFAs-producing bacteria Faecalibaculum, Bacteroides, and Romboutsia were increased significantly in the LOW group.					
35807944	3	59	theme	bowel	390:394	arg1	disease					396:402	inflammatory bowel disease	377:402	inflammatory bowel disease	377:402	However, the role of sucrose in inflammatory bowel disease remains controversial.					
35807944	4	60	theme	doses	491:495	arg1	role					477:480	the potential role	463:480	the potential role of three doses of sucrose on DSS-induced colitis in C57BL/6 mice	463:545	Hence, our study aimed to elucidate the potential role of three doses of sucrose on DSS-induced colitis in C57BL/6 mice and the underlying mechanisms.					
35807944	4	60	theme	doses	491:495	arg1	mechanisms					566:575	the underlying mechanisms	551:575	the underlying mechanisms	551:575	Hence, our study aimed to elucidate the potential role of three doses of sucrose on DSS-induced colitis in C57BL/6 mice and the underlying mechanisms.					
35807944	2	61	theme	consumed	247:254	arg1	ingredient					256:265	the widely consumed ingredient	236:265	the widely consumed ingredient in our daily diet	236:283	Sucrose, as the widely consumed ingredient in our daily diet, has been reported a relation to gastrointestinal diseases.					
35807944	8	62	theme	fatty	1006:1010	arg1	SCFAs					1019:1023	SCFAs	1019:1023	SCFAs	1019:1023	Notably, the low-dose sucrose restored microbial dysfunction and enhanced the production of short chain fatty acids (SCFAs).					
35807944	8	62	theme	fatty	1006:1010	arg1	acids					1012:1016	short chain fatty acids	994:1016	short chain fatty acids (SCFAs)	994:1024	Notably, the low-dose sucrose restored microbial dysfunction and enhanced the production of short chain fatty acids (SCFAs).					
36839244	0	0	theme	Infants	80:86	arg1	Feces					60:64	Feces	60:64	Feces	60:64	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	0	0	theme	Infants	80:86	arg1	Milk					51:54	Breast Milk	44:54	Breast Milk	44:54	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	1	1	theme	metabolic	290:298	arg1	fate					300:303	their metabolic fate	284:303	breast-fed babies' feces as well as their metabolic fate	248:303	Currently, it must be acknowledged that little is known about the quantity and make-up of oligosaccharides (OS) found in breast-fed babies' feces as well as their metabolic fate.					
36839244	7	2	theme	HMOs	1178:1181	arg1	profiles					1183:1190	HMOs profiles	1178:1190	HMOs profiles	1178:1190	Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.					
36839244	0	3	theme	Breast-Fed	69:78	arg1	Infants					80:86	Breast-Fed Infants	69:86	Breast-Fed Infants	69:86	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	0	4	from	Analysis	12:19	arg1	Feces					60:64	Feces	60:64	Feces	60:64	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	0	4	from	Analysis	12:19	arg1	Milk					51:54	Breast Milk	44:54	Breast Milk	44:54	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	7	5	theme	profiles	1183:1190	arg1	bioconversion					1161:1173	the gastrointestinal bioconversion	1140:1173	the gastrointestinal bioconversion of HMOs profiles	1140:1190	Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.					
36839244	2	6	from	feces	484:488	arg1	milk					443:446	the breast milk	432:446	the breast milk of four mothers and fecal OS in the feces of their breast-fed infant	432:515	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	5	7	theme	OS	921:922	arg1	profiles					924:931	fecal OS profiles	915:931	fecal OS profiles	915:931	The fate of HMOs during passage through the gastrointestinal tract may be profoundly informed by the comparison of OS between breast milk and fecal OS profiles.					
36839244	4	8	theme	HMOs	767:770	arg1	bioconversion					737:749	bioconversion	737:749	bioconversion	737:749	The early-life gastrointestinal microbiota metabolism may be triggered into the advanced breakdown, synthesis, bioconversion, or redesign of HMOs.					
36839244	4	8	theme	HMOs	767:770	arg1	redesign					755:762	redesign	755:762	redesign	755:762	The early-life gastrointestinal microbiota metabolism may be triggered into the advanced breakdown, synthesis, bioconversion, or redesign of HMOs.					
36839244	4	8	theme	HMOs	767:770	arg1	synthesis					726:734	synthesis	726:734	synthesis	726:734	The early-life gastrointestinal microbiota metabolism may be triggered into the advanced breakdown, synthesis, bioconversion, or redesign of HMOs.					
36839244	4	8	theme	HMOs	767:770	arg1	breakdown					715:723	the advanced breakdown	702:723	the advanced breakdown	702:723	The early-life gastrointestinal microbiota metabolism may be triggered into the advanced breakdown, synthesis, bioconversion, or redesign of HMOs.					
36839244	4	9	theme	advanced	706:713	arg1	breakdown					715:723	the advanced breakdown	702:723	the advanced breakdown	702:723	The early-life gastrointestinal microbiota metabolism may be triggered into the advanced breakdown, synthesis, bioconversion, or redesign of HMOs.					
36839244	2	10	theme	mothers	456:462	arg1	milk					443:446	the breast milk	432:446	the breast milk of four mothers and fecal OS in the feces of their breast-fed infant	432:515	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	3	11	theme	fecal	616:620	arg1	OS					622:623	fecal OS	616:623	fecal OS	616:623	There were significant variations and differences in both number and composition between HMOs and fecal OS.					
36839244	2	12	theme	infant	510:515	arg1	feces					484:488	the feces	480:488	the feces of their breast-fed infant	480:515	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	5	13	theme	breast	899:904	arg1	milk					906:909	breast milk	899:909	breast milk	899:909	The fate of HMOs during passage through the gastrointestinal tract may be profoundly informed by the comparison of OS between breast milk and fecal OS profiles.					
36839244	0	14	from	Milk	51:54	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.	0:125	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	3	15	from	variations	541:550	arg1	number					576:581	number	576:581	number	576:581	There were significant variations and differences in both number and composition between HMOs and fecal OS.					
36839244	3	15	from	variations	541:550	arg1	composition					587:597	composition	587:597	composition	587:597	There were significant variations and differences in both number and composition between HMOs and fecal OS.					
36839244	5	16	theme	HMOs	785:788	arg1	fate					777:780	The fate	773:780	The fate of HMOs during passage through the gastrointestinal tract	773:838	The fate of HMOs during passage through the gastrointestinal tract may be profoundly informed by the comparison of OS between breast milk and fecal OS profiles.					
36839244	7	17	theme	Further	1120:1126	arg1	research					1128:1135	Further research	1120:1135	Further research on the gastrointestinal bioconversion of HMOs profiles	1120:1190	Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.					
36839244	2	18	theme	human	394:398	arg1	HMOs					423:426	HMOs	423:426	HMOs	423:426	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	2	18	theme	human	394:398	arg1	oligosaccharides					405:420	human milk oligosaccharides	394:420	human milk oligosaccharides (HMOs)	394:427	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	6	19	theme	valuable	989:996	arg1	characterization					938:953	The characterization	934:953	The characterization of fecal OS	934:965	The characterization of fecal OS could be applied as a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation.					
36839244	6	19	theme	valuable	989:996	arg1	tool					998:1001	a valuable tool	987:1001	a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation	987:1117	The characterization of fecal OS could be applied as a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation.					
36839244	2	20	theme	milk	400:403	arg1	HMOs					423:426	HMOs	423:426	HMOs	423:426	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	2	20	theme	milk	400:403	arg1	oligosaccharides					405:420	human milk oligosaccharides	394:420	human milk oligosaccharides (HMOs)	394:427	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	7	21	theme	gastrointestinal	1144:1159	arg1	bioconversion					1161:1173	the gastrointestinal bioconversion	1140:1173	the gastrointestinal bioconversion of HMOs profiles	1140:1190	Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.					
36839244	1	22	located	found	239:243	arg1	fate					300:303	their metabolic fate	284:303	breast-fed babies' feces as well as their metabolic fate	248:303	Currently, it must be acknowledged that little is known about the quantity and make-up of oligosaccharides (OS) found in breast-fed babies' feces as well as their metabolic fate.					
36839244	1	22	located	found	239:243	arg2	OS					235:236	OS	235:236	OS	235:236	Currently, it must be acknowledged that little is known about the quantity and make-up of oligosaccharides (OS) found in breast-fed babies' feces as well as their metabolic fate.					
36839244	1	22	located	found	239:243	arg2	oligosaccharides					217:232	oligosaccharides	217:232	oligosaccharides (OS) found in breast-fed babies' feces as well as their metabolic fate	217:303	Currently, it must be acknowledged that little is known about the quantity and make-up of oligosaccharides (OS) found in breast-fed babies' feces as well as their metabolic fate.					
36839244	1	22	located	found	239:243	arg1	feces					267:271	breast-fed babies' feces	248:271	breast-fed babies' feces as well as their metabolic fate	248:303	Currently, it must be acknowledged that little is known about the quantity and make-up of oligosaccharides (OS) found in breast-fed babies' feces as well as their metabolic fate.					
36839244	3	23	from	differences	556:566	arg1	number					576:581	number	576:581	number	576:581	There were significant variations and differences in both number and composition between HMOs and fecal OS.					
36839244	3	23	from	differences	556:566	arg1	composition					587:597	composition	587:597	composition	587:597	There were significant variations and differences in both number and composition between HMOs and fecal OS.					
36839244	7	24	from	research	1128:1135	arg1	bioconversion					1161:1173	the gastrointestinal bioconversion	1140:1173	the gastrointestinal bioconversion of HMOs profiles	1140:1190	Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.					
36839244	0	25	theme	Comparative	0:10	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.	0:125	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	7	26	theme	feeding	1277:1283	arg1	time					1247:1250	the lactation time	1233:1250	the lactation time of milk, as well as baby feeding	1233:1283	Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.					
36839244	7	26	theme	feeding	1277:1283	arg1	type					1224:1227	secretor type	1215:1227	secretor type	1215:1227	Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.					
36839244	7	27	theme	milk	1255:1258	arg1	time					1247:1250	the lactation time	1233:1250	the lactation time of milk, as well as baby feeding	1233:1283	Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.					
36839244	7	27	theme	milk	1255:1258	arg1	type					1224:1227	secretor type	1215:1227	secretor type	1215:1227	Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.					
36839244	0	28	theme	Oligosaccharides	24:39	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.	0:125	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	1	29	theme	breast-fed	248:257	arg1	babies					259:264	breast-fed babies'	248:265	breast-fed babies' feces as well as their metabolic fate	248:303	Currently, it must be acknowledged that little is known about the quantity and make-up of oligosaccharides (OS) found in breast-fed babies' feces as well as their metabolic fate.					
36839244	6	30	theme	infant	1067:1072	arg1	development					1074:1084	infant development	1067:1084	infant development	1067:1084	The characterization of fecal OS could be applied as a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation.					
36839244	7	31	theme	lactation	1237:1245	arg1	time					1247:1250	the lactation time	1233:1250	the lactation time of milk, as well as baby feeding	1233:1283	Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.					
36839244	6	32	theme	lactation	1109:1117	arg1	stages					1099:1104	different stages	1089:1104	different stages of lactation	1089:1117	The characterization of fecal OS could be applied as a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation.					
36839244	5	33	theme	OS	888:889	arg1	comparison					874:883	the comparison	870:883	the comparison of OS between breast milk and fecal OS profiles	870:931	The fate of HMOs during passage through the gastrointestinal tract may be profoundly informed by the comparison of OS between breast milk and fecal OS profiles.					
36839244	6	34	theme	gastrointestinal	1022:1037	arg1	fate					1039:1042	the gastrointestinal fate	1018:1042	the gastrointestinal fate of HMOs	1018:1050	The characterization of fecal OS could be applied as a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation.					
36839244	0	35	theme	Breast	44:49	arg1	Milk					51:54	Breast Milk	44:54	Breast Milk	44:54	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	6	36	theme	HMOs	1047:1050	arg1	fate					1039:1042	the gastrointestinal fate	1018:1042	the gastrointestinal fate of HMOs	1018:1050	The characterization of fecal OS could be applied as a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation.					
36839244	2	37	theme	OS	474:475	arg1	milk					443:446	the breast milk	432:446	the breast milk of four mothers and fecal OS in the feces of their breast-fed infant	432:515	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	6	38	theme	fecal	958:962	arg1	OS					964:965	fecal OS	958:965	fecal OS	958:965	The characterization of fecal OS could be applied as a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation.					
36839244	2	39	theme	fecal	468:472	arg1	OS					474:475	fecal OS	468:475	fecal OS in the feces of their breast-fed infant	468:515	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	2	40	from	milk	443:446	arg1	feces					484:488	the feces	480:488	the feces of their breast-fed infant	480:515	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	6	41	theme	OS	964:965	arg1	characterization					938:953	The characterization	934:953	The characterization of fecal OS	934:965	The characterization of fecal OS could be applied as a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation.					
36839244	6	41	theme	OS	964:965	arg1	tool					998:1001	a valuable tool	987:1001	a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation	987:1117	The characterization of fecal OS could be applied as a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation.					
36839244	2	42	theme	breast-fed	499:508	arg1	infant					510:515	their breast-fed infant	493:515	their breast-fed infant	493:515	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	0	43	from	Feces	60:64	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.	0:125	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	2	44	from	profiles	382:389	arg1	milk					443:446	the breast milk	432:446	the breast milk of four mothers and fecal OS in the feces of their breast-fed infant	432:515	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	4	45	theme	gastrointestinal	641:656	arg1	metabolism					669:678	The early-life gastrointestinal microbiota metabolism	626:678	The early-life gastrointestinal microbiota metabolism	626:678	The early-life gastrointestinal microbiota metabolism may be triggered into the advanced breakdown, synthesis, bioconversion, or redesign of HMOs.					
36839244	2	46	theme	oligosaccharides	405:420	arg1	profiles					382:389	the profiles	378:389	the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant	378:515	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	7	47	theme	secretor	1215:1222	arg1	type					1224:1227	secretor type	1215:1227	secretor type	1215:1227	Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.					
36839244	4	48	theme	early-life	630:639	arg1	metabolism					669:678	The early-life gastrointestinal microbiota metabolism	626:678	The early-life gastrointestinal microbiota metabolism	626:678	The early-life gastrointestinal microbiota metabolism may be triggered into the advanced breakdown, synthesis, bioconversion, or redesign of HMOs.					
36839244	7	49	theme	baby	1272:1275	arg1	feeding					1277:1283	baby feeding	1272:1283	baby feeding	1272:1283	Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.					
36839244	5	50	theme	fecal	915:919	arg1	profiles					924:931	fecal OS profiles	915:931	fecal OS profiles	915:931	The fate of HMOs during passage through the gastrointestinal tract may be profoundly informed by the comparison of OS between breast milk and fecal OS profiles.					
36839244	3	51	theme	significant	529:539	arg1	variations					541:550	significant variations	529:550	significant variations	529:550	There were significant variations and differences in both number and composition between HMOs and fecal OS.					
36839244	2	52	from	OS	474:475	arg1	feces					484:488	the feces	480:488	the feces of their breast-fed infant	480:515	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	6	53	theme	different	1089:1097	arg1	stages					1099:1104	different stages	1089:1104	different stages of lactation	1089:1117	The characterization of fecal OS could be applied as a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation.					
36839244	1	54	dep	quantity	193:200	arg1	the					189:191	the	189:191	the	189:191	Currently, it must be acknowledged that little is known about the quantity and make-up of oligosaccharides (OS) found in breast-fed babies' feces as well as their metabolic fate.					
36839244	4	55	theme	microbiota	658:667	arg1	metabolism					669:678	The early-life gastrointestinal microbiota metabolism	626:678	The early-life gastrointestinal microbiota metabolism	626:678	The early-life gastrointestinal microbiota metabolism may be triggered into the advanced breakdown, synthesis, bioconversion, or redesign of HMOs.					
36839244	2	56	from	mothers	456:462	arg1	feces					484:488	the feces	480:488	the feces of their breast-fed infant	480:515	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	0	57	dep	Analysis	12:19	arg1	Communication					112:124	A Communication	110:124	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.	0:125	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	5	58	theme	gastrointestinal	817:832	arg1	tract					834:838	the gastrointestinal tract	813:838	the gastrointestinal tract	813:838	The fate of HMOs during passage through the gastrointestinal tract may be profoundly informed by the comparison of OS between breast milk and fecal OS profiles.					
36839244	2	59	theme	breast	436:441	arg1	milk					443:446	the breast milk	432:446	the breast milk of four mothers and fecal OS in the feces of their breast-fed infant	432:515	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	0	60	from	Oligosaccharides	24:39	arg1	Feces					60:64	Feces	60:64	Feces	60:64	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	0	60	from	Oligosaccharides	24:39	arg1	Milk					51:54	Breast Milk	44:54	Breast Milk	44:54	Comparative Analysis of Oligosaccharides in Breast Milk and Feces of Breast-Fed Infants by Using LC-QE-HF-MS: A Communication.					
36839244	2	61	theme	present	313:319	arg1	work					321:324	the present work	309:324	the present work	309:324	In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant.					
36839244	1	62	theme	oligosaccharides	217:232	arg1	make-up					206:212	make-up	206:212	make-up	206:212	Currently, it must be acknowledged that little is known about the quantity and make-up of oligosaccharides (OS) found in breast-fed babies' feces as well as their metabolic fate.					
36839244	1	62	theme	oligosaccharides	217:232	arg1	quantity					193:200	quantity	193:200	quantity	193:200	Currently, it must be acknowledged that little is known about the quantity and make-up of oligosaccharides (OS) found in breast-fed babies' feces as well as their metabolic fate.					
35688402	6	0	theme	cross-linking	964:976	arg1	effect					948:953	Synergistic effect	936:953	Synergistic effect of ionic cross-linking and enzymatic mineralization	936:1005	Synergistic effect of ionic cross-linking and enzymatic mineralization was clearly observed with dramatic increase in compressive modulus.					
35688402	10	1	theme	cell	1628:1631	arg1	adhesion					1648:1655	adhesion	1648:1655	adhesion	1648:1655	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	10	1	theme	cell	1628:1631	arg1	result					1707:1712	a result	1705:1712	a result of enzymatic mineralization	1705:1740	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	10	1	theme	cell	1628:1631	arg1	differentiation					1672:1686	osteogenic differentiation	1661:1686	osteogenic differentiation	1661:1686	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	10	1	theme	cell	1628:1631	arg1	proliferation					1633:1645	cell proliferation	1628:1645	cell proliferation	1628:1645	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	15	2	theme	Such	2545:2548	arg1	effect					2562:2567	Such synergistic effect	2545:2567	Such synergistic effect from Sr<sup>2+</sup>	2545:2588	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process.					
35688402	9	3	theme	early	1490:1494	arg1	initiation					1496:1505	the early initiation	1486:1505	the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure	1486:1590	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	13	4	theme	synergistic	2125:2135	arg1	effect					2137:2142	the synergistic effect	2121:2142	the synergistic effect of ionic cross-linking and enzymatic mineralization	2121:2194	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	6	5	theme	Synergistic	936:946	arg1	effect					948:953	Synergistic effect	936:953	Synergistic effect of ionic cross-linking and enzymatic mineralization	936:1005	Synergistic effect of ionic cross-linking and enzymatic mineralization was clearly observed with dramatic increase in compressive modulus.					
35688402	8	6	theme	Young	1306:1310	arg1	modulus					1314:1320	tensile Young's modulus	1298:1320	the highest tensile Young's modulus	1286:1320	In addition, it had the highest tensile Young's modulus at 2.60 ± 0.25 MPa and 84 ± 5.5% elongation at break.					
35688402	11	7	theme	effective	1767:1775	arg1	way					1777:1779	an effective way	1764:1779	an effective way for the preparation of stiff and tough hydrogels with osteogenesis	1764:1846	Our results provided an effective way for the preparation of stiff and tough hydrogels with osteogenesis, and demonstrated potential in bone tissue engineering applications.					
35688402	2	8	theme	other	280:284	arg1	hand					286:289	the other hand	276:289	the other hand	276:289	On the other hand, double network cross-linking provides hydrogel with enhanced toughness.					
35688402	12	9	with	Hydrogels	1944:1952	arg1	biocompatibility					1994:2009	biocompatibility	1994:2009	biocompatibility	1994:2009	STATEMENT OF SIGNIFICANCE: Hydrogels with excellent stiffness, stability and biocompatibility have attracted significant attentions in the bone tissue engineering applications.					
35688402	12	9	with	Hydrogels	1944:1952	arg1	stability					1980:1988	stability	1980:1988	stability	1980:1988	STATEMENT OF SIGNIFICANCE: Hydrogels with excellent stiffness, stability and biocompatibility have attracted significant attentions in the bone tissue engineering applications.					
35688402	12	9	with	Hydrogels	1944:1952	arg1	stiffness					1969:1977	excellent stiffness	1959:1977	excellent stiffness	1959:1977	STATEMENT OF SIGNIFICANCE: Hydrogels with excellent stiffness, stability and biocompatibility have attracted significant attentions in the bone tissue engineering applications.					
35688402	9	10	theme	nucleation	1529:1538	arg1	process					1540:1546	a more homogeneous nucleation process	1510:1546	a more homogeneous nucleation process	1510:1546	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	6	11	theme	dramatic	1033:1040	arg1	increase					1042:1049	dramatic increase	1033:1049	dramatic increase in compressive modulus	1033:1072	Synergistic effect of ionic cross-linking and enzymatic mineralization was clearly observed with dramatic increase in compressive modulus.					
35688402	15	12	from	Sr<sup>2+</sup>	2574:2588	arg1	effect					2562:2567	Such synergistic effect	2545:2567	Such synergistic effect from Sr<sup>2+</sup>	2545:2588	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process.					
35688402	16	13	theme	cell	2653:2656	arg1	differentiation					2697:2711	osteogenic differentiation	2686:2711	osteogenic differentiation	2686:2711	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	16	13	theme	cell	2653:2656	arg1	proliferation					2658:2670	The cell proliferation	2649:2670	The cell proliferation	2649:2670	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	16	13	theme	cell	2653:2656	arg1	result					2740:2745	a result	2738:2745	a result	2738:2745	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	16	13	theme	cell	2653:2656	arg1	adhesion					2673:2680	adhesion	2673:2680	adhesion	2673:2680	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	4	14	theme	biological	690:699	arg1	properties					701:710	biological properties	690:710	biological properties	690:710	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	7	15	theme	compressive	1164:1174	arg1	modulus					1184:1190	compressive Young's modulus	1164:1190	compressive Young's modulus	1164:1190	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56 MPa, which was 37 times of that of the original hydrogel.					
35688402	7	15	theme	compressive	1164:1174	arg1	times					1226:1230	37 times	1223:1230	37 times of that of the original hydrogel	1223:1263	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56 MPa, which was 37 times of that of the original hydrogel.					
35688402	15	16	theme	synergistic	2550:2560	arg1	effect					2562:2567	Such synergistic effect	2545:2567	Such synergistic effect from Sr<sup>2+</sup>	2545:2588	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process.					
35688402	2	17	theme	network	299:305	arg1	cross-linking					307:319	double network cross-linking	292:319	double network cross-linking	292:319	On the other hand, double network cross-linking provides hydrogel with enhanced toughness.					
35688402	5	18	theme	strontium	878:886	arg1	ions					888:891	calcium, zinc and strontium ions	860:891	calcium, zinc and strontium ions	860:891	In particular, we examined the role of specific cross-linking ions, i.e., calcium, zinc and strontium ions, in modulating the mineralization process.					
35688402	16	19	theme	strontium	2803:2811	arg1	hydrogel					2842:2849	strontium ion cross-linked mineralized hydrogel	2803:2849	strontium ion cross-linked mineralized hydrogel	2803:2849	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	8	20	theme	84 ± 5.5	1345:1352	arg1	%					1353:1353	%	1353:1353	%	1353:1353	In addition, it had the highest tensile Young's modulus at 2.60 ± 0.25 MPa and 84 ± 5.5% elongation at break.					
35688402	10	21	theme	mineralization	1727:1740	arg1	adhesion					1648:1655	adhesion	1648:1655	adhesion	1648:1655	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	10	21	theme	mineralization	1727:1740	arg1	result					1707:1712	a result	1705:1712	a result of enzymatic mineralization	1705:1740	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	10	21	theme	mineralization	1727:1740	arg1	differentiation					1672:1686	osteogenic differentiation	1661:1686	osteogenic differentiation	1661:1686	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	10	21	theme	mineralization	1727:1740	arg1	proliferation					1633:1645	cell proliferation	1628:1645	cell proliferation	1628:1645	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	9	22	theme	denser	1563:1568	arg1	structure					1582:1590	subsequent denser mineralized structure	1552:1590	subsequent denser mineralized structure	1552:1590	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	8	23	theme	highest	1290:1296	arg1	modulus					1314:1320	tensile Young's modulus	1298:1320	the highest tensile Young's modulus	1286:1320	In addition, it had the highest tensile Young's modulus at 2.60 ± 0.25 MPa and 84 ± 5.5% elongation at break.					
35688402	16	24	theme	cross-linked	2817:2828	arg1	hydrogel					2842:2849	strontium ion cross-linked mineralized hydrogel	2803:2849	strontium ion cross-linked mineralized hydrogel	2803:2849	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	4	25	theme	mineralization	770:783	arg1	presence					758:765	presence	758:765	presence	758:765	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	4	25	theme	mineralization	770:783	arg1	absence					746:752	absence	746:752	absence	746:752	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	13	26	theme	SA-PAM	2266:2271	arg1	hydrogels					2276:2284	SA-PAM DN hydrogels	2266:2284	SA-PAM DN hydrogels	2266:2284	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	8	27	contain	had	1282:1284	arg2	modulus					1314:1320	tensile Young's modulus	1298:1320	the highest tensile Young's modulus	1286:1320	In addition, it had the highest tensile Young's modulus at 2.60 ± 0.25 MPa and 84 ± 5.5% elongation at break.					
35688402	8	27	contain	had	1282:1284	arg1	it					1279:1280	it	1279:1280	it	1279:1280	In addition, it had the highest tensile Young's modulus at 2.60 ± 0.25 MPa and 84 ± 5.5% elongation at break.					
35688402	9	28	theme	structure	1582:1590	arg1	initiation					1496:1505	the early initiation	1486:1505	the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure	1486:1590	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	6	29	theme	enzymatic	982:990	arg1	mineralization					992:1005	enzymatic mineralization	982:1005	enzymatic mineralization	982:1005	Synergistic effect of ionic cross-linking and enzymatic mineralization was clearly observed with dramatic increase in compressive modulus.					
35688402	13	30	theme	hydrogels	2276:2284	arg1	stiffness					2253:2261	the compressive and tensile stiffness	2225:2261	the compressive and tensile stiffness of SA-PAM DN hydrogels	2225:2284	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	1	31	theme	effective	147:155	arg1	approach					157:164	an effective approach	144:164	an effective approach to enhancing the stiffness of hydrogels for bone tissue engineering, but generally with limited toughness	144:270	Enzymatic mineralization has become an effective approach to enhancing the stiffness of hydrogels for bone tissue engineering, but generally with limited toughness.					
35688402	0	32	theme	Ionic	58:62	arg1	Cross-linking					64:76	Ionic Cross-linking	58:76	Ionic Cross-linking	58:76	Stiff and Tough Hydrogels Prepared Through Integration of Ionic Cross-linking and Enzymatic Mineralization.					
35688402	15	33	theme	mineralization	2625:2638	arg1	process					2640:2646	a more uniformed mineralization process	2608:2646	a more uniformed mineralization process	2608:2646	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process.					
35688402	16	34	theme	enzymatic	2750:2758	arg1	mineralization					2760:2773	enzymatic mineralization	2750:2773	enzymatic mineralization	2750:2773	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	11	35	theme	tissue	1884:1889	arg1	engineering					1891:1901	bone tissue engineering	1879:1901	bone tissue engineering	1879:1901	Our results provided an effective way for the preparation of stiff and tough hydrogels with osteogenesis, and demonstrated potential in bone tissue engineering applications.					
35688402	5	36	theme	specific	825:832	arg1	ions					848:851	specific cross-linking ions	825:851	specific cross-linking ions	825:851	In particular, we examined the role of specific cross-linking ions, i.e., calcium, zinc and strontium ions, in modulating the mineralization process.					
35688402	13	37	theme	stiffness	2253:2261	arg1	enhancement					2210:2220	more enhancement	2205:2220	more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels	2205:2284	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	13	37	theme	stiffness	2253:2261	arg1	toughness					2302:2310	the toughness	2298:2310	the toughness	2298:2310	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	12	38	theme	bone	2056:2059	arg1	applications					2080:2091	the bone tissue engineering applications	2052:2091	the bone tissue engineering applications	2052:2091	STATEMENT OF SIGNIFICANCE: Hydrogels with excellent stiffness, stability and biocompatibility have attracted significant attentions in the bone tissue engineering applications.					
35688402	4	39	theme	different	719:727	arg1	hydrogels					729:737	the different hydrogels	715:737	the different hydrogels	715:737	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	0	40	theme	Mineralization	92:105	arg1	Integration					43:53	Integration	43:53	Integration of Ionic Cross-linking and Enzymatic Mineralization	43:105	Stiff and Tough Hydrogels Prepared Through Integration of Ionic Cross-linking and Enzymatic Mineralization.					
35688402	12	41	theme	engineering	2068:2078	arg1	applications					2080:2091	the bone tissue engineering applications	2052:2091	the bone tissue engineering applications	2052:2091	STATEMENT OF SIGNIFICANCE: Hydrogels with excellent stiffness, stability and biocompatibility have attracted significant attentions in the bone tissue engineering applications.					
35688402	12	42	dep	STATEMENT	1917:1925	arg1	attracted					2016:2024	attracted	2016:2024	have attracted significant attentions in the bone tissue engineering applications	2011:2091	STATEMENT OF SIGNIFICANCE: Hydrogels with excellent stiffness, stability and biocompatibility have attracted significant attentions in the bone tissue engineering applications.					
35688402	3	43	theme	stiff	465:469	arg1	hydrogels					481:489	stiff and tough hydrogels	465:489	stiff and tough hydrogels	465:489	In this study, we integrated double cross-linking method with enzymatic mineralization to synthesize stiff and tough hydrogels.					
35688402	9	44	theme	synergistic	1381:1391	arg1	effect					1393:1398	Such synergistic effect	1376:1398	Such synergistic effect from Sr<sup>2+</sup>	1376:1419	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	12	45	theme	significant	2026:2036	arg1	attentions					2038:2047	significant attentions	2026:2047	significant attentions	2026:2047	STATEMENT OF SIGNIFICANCE: Hydrogels with excellent stiffness, stability and biocompatibility have attracted significant attentions in the bone tissue engineering applications.					
35688402	3	46	theme	double	393:398	arg1	method					414:419	double cross-linking method	393:419	double cross-linking method	393:419	In this study, we integrated double cross-linking method with enzymatic mineralization to synthesize stiff and tough hydrogels.					
35688402	14	47	theme	Young	2456:2460	arg1	times					2505:2509	37 times	2502:2509	37 times of that of the original hydrogel	2502:2542	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56MPa, which was 37 times of that of the original hydrogel.					
35688402	14	47	theme	Young	2456:2460	arg1	modulus					2464:2470	compressive Young's modulus	2444:2470	compressive Young's modulus	2444:2470	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56MPa, which was 37 times of that of the original hydrogel.					
35688402	5	48	theme	calcium	860:866	arg1	ions					888:891	calcium, zinc and strontium ions	860:891	calcium, zinc and strontium ions	860:891	In particular, we examined the role of specific cross-linking ions, i.e., calcium, zinc and strontium ions, in modulating the mineralization process.					
35688402	9	49	from	Sr<sup>2+</sup>	1405:1419	arg1	effect					1393:1398	Such synergistic effect	1376:1398	Such synergistic effect from Sr<sup>2+</sup>	1376:1419	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	10	50	theme	Cellular	1593:1600	arg1	study					1602:1606	Cellular study	1593:1606	Cellular study	1593:1606	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	14	51	theme	highest	2436:2442	arg1	times					2505:2509	37 times	2502:2509	37 times of that of the original hydrogel	2502:2542	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56MPa, which was 37 times of that of the original hydrogel.					
35688402	14	51	theme	highest	2436:2442	arg1	modulus					2464:2470	compressive Young's modulus	2444:2470	compressive Young's modulus	2444:2470	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56MPa, which was 37 times of that of the original hydrogel.					
35688402	5	52	theme	zinc	869:872	arg1	ions					888:891	calcium, zinc and strontium ions	860:891	calcium, zinc and strontium ions	860:891	In particular, we examined the role of specific cross-linking ions, i.e., calcium, zinc and strontium ions, in modulating the mineralization process.					
35688402	16	53	theme	osteogenic	2686:2695	arg1	differentiation					2697:2711	osteogenic differentiation	2686:2711	osteogenic differentiation	2686:2711	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	16	53	theme	osteogenic	2686:2695	arg1	proliferation					2658:2670	The cell proliferation	2649:2670	The cell proliferation	2649:2670	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	16	53	theme	osteogenic	2686:2695	arg1	result					2740:2745	a result	2738:2745	a result	2738:2745	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	16	53	theme	osteogenic	2686:2695	arg1	adhesion					2673:2680	adhesion	2673:2680	adhesion	2673:2680	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	3	54	theme	enzymatic	426:434	arg1	mineralization					436:449	enzymatic mineralization	426:449	enzymatic mineralization	426:449	In this study, we integrated double cross-linking method with enzymatic mineralization to synthesize stiff and tough hydrogels.					
35688402	11	55	with	hydrogels	1820:1828	arg1	osteogenesis					1835:1846	osteogenesis	1835:1846	osteogenesis	1835:1846	Our results provided an effective way for the preparation of stiff and tough hydrogels with osteogenesis, and demonstrated potential in bone tissue engineering applications.					
35688402	6	56	from	increase	1042:1049	arg1	modulus					1066:1072	compressive modulus	1054:1072	compressive modulus	1054:1072	Synergistic effect of ionic cross-linking and enzymatic mineralization was clearly observed with dramatic increase in compressive modulus.					
35688402	3	57	theme	tough	475:479	arg1	hydrogels					481:489	stiff and tough hydrogels	465:489	stiff and tough hydrogels	465:489	In this study, we integrated double cross-linking method with enzymatic mineralization to synthesize stiff and tough hydrogels.					
35688402	7	58	theme	that	1235:1238	arg1	modulus					1184:1190	compressive Young's modulus	1164:1190	compressive Young's modulus	1164:1190	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56 MPa, which was 37 times of that of the original hydrogel.					
35688402	7	58	theme	that	1235:1238	arg1	times					1226:1230	37 times	1223:1230	37 times of that of the original hydrogel	1223:1263	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56 MPa, which was 37 times of that of the original hydrogel.					
35688402	5	59	theme	ions	848:851	arg1	role					817:820	the role	813:820	the role of specific cross-linking ions, i.e., calcium, zinc and strontium ions, in modulating the mineralization process	813:933	In particular, we examined the role of specific cross-linking ions, i.e., calcium, zinc and strontium ions, in modulating the mineralization process.					
35688402	13	60	theme	mineralization	2181:2194	arg1	effect					2137:2142	the synergistic effect	2121:2142	the synergistic effect of ionic cross-linking and enzymatic mineralization	2121:2194	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	12	61	theme	excellent	1959:1967	arg1	stiffness					1969:1977	excellent stiffness	1959:1977	excellent stiffness	1959:1977	STATEMENT OF SIGNIFICANCE: Hydrogels with excellent stiffness, stability and biocompatibility have attracted significant attentions in the bone tissue engineering applications.					
35688402	4	62	theme	double-network	567:580	arg1	hydrogels					582:590	sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels	527:590	sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels	527:590	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	5	63	dep	ions	888:891	arg1	i.e.					854:857	i.e.	854:857	i.e.	854:857	In particular, we examined the role of specific cross-linking ions, i.e., calcium, zinc and strontium ions, in modulating the mineralization process.					
35688402	13	64	theme	more	2205:2208	arg1	enhancement					2210:2220	more enhancement	2205:2220	more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels	2205:2284	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	2	65	theme	enhanced	344:351	arg1	toughness					353:361	enhanced toughness	344:361	enhanced toughness	344:361	On the other hand, double network cross-linking provides hydrogel with enhanced toughness.					
35688402	9	66	theme	mineralization	1456:1469	arg1	process					1471:1477	a more uniformed mineralization process	1439:1477	a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure	1439:1590	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	11	67	theme	hydrogels	1820:1828	arg1	preparation					1789:1799	the preparation	1785:1799	the preparation of stiff and tough hydrogels with osteogenesis	1785:1846	Our results provided an effective way for the preparation of stiff and tough hydrogels with osteogenesis, and demonstrated potential in bone tissue engineering applications.					
35688402	13	68	theme	ionic	2147:2151	arg1	cross-linking					2153:2165	ionic cross-linking	2147:2165	ionic cross-linking	2147:2165	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	9	69	theme	due	1479:1481	arg1	process					1471:1477	a more uniformed mineralization process	1439:1477	a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure	1439:1590	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	15	70	theme	uniformed	2615:2623	arg1	process					2640:2646	a more uniformed mineralization process	2608:2646	a more uniformed mineralization process	2608:2646	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process.					
35688402	6	71	theme	ionic	958:962	arg1	cross-linking					964:976	ionic cross-linking	958:976	ionic cross-linking	958:976	Synergistic effect of ionic cross-linking and enzymatic mineralization was clearly observed with dramatic increase in compressive modulus.					
35688402	14	72	theme	mineralized	2370:2380	arg1	hydrogel					2382:2389	mineralized hydrogel	2370:2389	mineralized hydrogel cross-linked with Sr<sup>2+</sup>	2370:2423	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56MPa, which was 37 times of that of the original hydrogel.					
35688402	7	73	theme	17.28 ± 3.56 MPa	1195:1210	arg1	modulus					1184:1190	compressive Young's modulus	1164:1190	compressive Young's modulus	1164:1190	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56 MPa, which was 37 times of that of the original hydrogel.					
35688402	7	73	theme	17.28 ± 3.56 MPa	1195:1210	arg1	times					1226:1230	37 times	1223:1230	37 times of that of the original hydrogel	1223:1263	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56 MPa, which was 37 times of that of the original hydrogel.					
35688402	2	74	theme	double	292:297	arg1	cross-linking					307:319	double network cross-linking	292:319	double network cross-linking	292:319	On the other hand, double network cross-linking provides hydrogel with enhanced toughness.					
35688402	10	75	theme	osteogenic	1661:1670	arg1	adhesion					1648:1655	adhesion	1648:1655	adhesion	1648:1655	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	10	75	theme	osteogenic	1661:1670	arg1	result					1707:1712	a result	1705:1712	a result of enzymatic mineralization	1705:1740	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	10	75	theme	osteogenic	1661:1670	arg1	differentiation					1672:1686	osteogenic differentiation	1661:1686	osteogenic differentiation	1661:1686	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	10	75	theme	osteogenic	1661:1670	arg1	proliferation					1633:1645	cell proliferation	1628:1645	cell proliferation	1628:1645	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	1	76	theme	hydrogels	196:204	arg1	stiffness					183:191	the stiffness	179:191	the stiffness of hydrogels for bone tissue engineering, but generally with limited toughness	179:270	Enzymatic mineralization has become an effective approach to enhancing the stiffness of hydrogels for bone tissue engineering, but generally with limited toughness.					
35688402	8	77	theme	tensile	1298:1304	arg1	modulus					1314:1320	tensile Young's modulus	1298:1320	the highest tensile Young's modulus	1286:1320	In addition, it had the highest tensile Young's modulus at 2.60 ± 0.25 MPa and 84 ± 5.5% elongation at break.					
35688402	7	78	theme	Young	1176:1180	arg1	modulus					1184:1190	compressive Young's modulus	1164:1190	compressive Young's modulus	1164:1190	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56 MPa, which was 37 times of that of the original hydrogel.					
35688402	7	78	theme	Young	1176:1180	arg1	times					1226:1230	37 times	1223:1230	37 times of that of the original hydrogel	1223:1263	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56 MPa, which was 37 times of that of the original hydrogel.					
35688402	14	79	theme	original	2526:2533	arg1	hydrogel					2535:2542	the original hydrogel	2522:2542	the original hydrogel	2522:2542	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56MPa, which was 37 times of that of the original hydrogel.					
35688402	9	80	theme	homogeneous	1517:1527	arg1	process					1540:1546	a more homogeneous nucleation process	1510:1546	a more homogeneous nucleation process	1510:1546	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	4	81	theme	hydrogels	729:737	arg1	properties					701:710	biological properties	690:710	biological properties	690:710	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	4	81	theme	hydrogels	729:737	arg1	structure					640:648	structure	640:648	structure	640:648	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	4	81	theme	hydrogels	729:737	arg1	composition					624:634	composition	624:634	composition	624:634	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	4	81	theme	hydrogels	729:737	arg1	properties					674:683	mechanical properties	663:683	mechanical properties	663:683	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	7	82	theme	highest	1156:1162	arg1	modulus					1184:1190	compressive Young's modulus	1164:1190	compressive Young's modulus	1164:1190	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56 MPa, which was 37 times of that of the original hydrogel.					
35688402	7	82	theme	highest	1156:1162	arg1	times					1226:1230	37 times	1223:1230	37 times of that of the original hydrogel	1223:1263	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56 MPa, which was 37 times of that of the original hydrogel.					
35688402	9	83	theme	process	1540:1546	arg1	initiation					1496:1505	the early initiation	1486:1505	the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure	1486:1590	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	16	84	theme	ion	2813:2815	arg1	hydrogel					2842:2849	strontium ion cross-linked mineralized hydrogel	2803:2849	strontium ion cross-linked mineralized hydrogel	2803:2849	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	16	85	link	cross-linked	2817:2828	arg1	hydrogel					2842:2849	strontium ion cross-linked mineralized hydrogel	2803:2849	strontium ion cross-linked mineralized hydrogel	2803:2849	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	5	86	theme	mineralization	912:925	arg1	process					927:933	the mineralization process	908:933	the mineralization process	908:933	In particular, we examined the role of specific cross-linking ions, i.e., calcium, zinc and strontium ions, in modulating the mineralization process.					
35688402	9	87	theme	subsequent	1552:1561	arg1	structure					1582:1590	subsequent denser mineralized structure	1552:1590	subsequent denser mineralized structure	1552:1590	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	16	88	theme	mineralized	2830:2840	arg1	hydrogel					2842:2849	strontium ion cross-linked mineralized hydrogel	2803:2849	strontium ion cross-linked mineralized hydrogel	2803:2849	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	11	89	from	engineering	1891:1901	arg1	potential					1866:1874	potential	1866:1874	potential	1866:1874	Our results provided an effective way for the preparation of stiff and tough hydrogels with osteogenesis, and demonstrated potential in bone tissue engineering applications.					
35688402	10	90	theme	enzymatic	1717:1725	arg1	mineralization					1727:1740	enzymatic mineralization	1717:1740	enzymatic mineralization	1717:1740	Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.					
35688402	4	91	dep	composition	624:634	arg1	differences					650:660	differences	650:660	differences	650:660	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	7	92	theme	mineralized	1090:1100	arg1	hydrogel					1102:1109	mineralized hydrogel	1090:1109	mineralized hydrogel cross-linked with Sr<sup>2+</sup>	1090:1143	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56 MPa, which was 37 times of that of the original hydrogel.					
35688402	9	93	theme	mineralized	1570:1580	arg1	structure					1582:1590	subsequent denser mineralized structure	1552:1590	subsequent denser mineralized structure	1552:1590	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	6	94	theme	mineralization	992:1005	arg1	effect					948:953	Synergistic effect	936:953	Synergistic effect of ionic cross-linking and enzymatic mineralization	936:1005	Synergistic effect of ionic cross-linking and enzymatic mineralization was clearly observed with dramatic increase in compressive modulus.					
35688402	8	95	theme	%	1353:1353	arg1	elongation					1355:1364	84 ± 5.5% elongation	1345:1364	84 ± 5.5% elongation	1345:1364	In addition, it had the highest tensile Young's modulus at 2.60 ± 0.25 MPa and 84 ± 5.5% elongation at break.					
35688402	14	96	theme	that	2514:2517	arg1	times					2505:2509	37 times	2502:2509	37 times of that of the original hydrogel	2502:2542	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56MPa, which was 37 times of that of the original hydrogel.					
35688402	14	96	theme	that	2514:2517	arg1	modulus					2464:2470	compressive Young's modulus	2444:2470	compressive Young's modulus	2444:2470	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56MPa, which was 37 times of that of the original hydrogel.					
35688402	13	97	theme	DN	2273:2274	arg1	hydrogels					2276:2284	SA-PAM DN hydrogels	2266:2284	SA-PAM DN hydrogels	2266:2284	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	11	98	from	potential	1866:1874	arg1	engineering					1891:1901	bone tissue engineering	1879:1901	bone tissue engineering	1879:1901	Our results provided an effective way for the preparation of stiff and tough hydrogels with osteogenesis, and demonstrated potential in bone tissue engineering applications.					
35688402	11	99	theme	potential	1866:1874	arg1	applications					1903:1914	potential in bone tissue engineering applications	1866:1914	potential in bone tissue engineering applications	1866:1914	Our results provided an effective way for the preparation of stiff and tough hydrogels with osteogenesis, and demonstrated potential in bone tissue engineering applications.					
35688402	0	100	theme	Cross-linking	64:76	arg1	Integration					43:53	Integration	43:53	Integration of Ionic Cross-linking and Enzymatic Mineralization	43:105	Stiff and Tough Hydrogels Prepared Through Integration of Ionic Cross-linking and Enzymatic Mineralization.					
35688402	14	101	theme	17.28 ± 3.56MPa	2475:2489	arg1	times					2505:2509	37 times	2502:2509	37 times of that of the original hydrogel	2502:2542	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56MPa, which was 37 times of that of the original hydrogel.					
35688402	14	101	theme	17.28 ± 3.56MPa	2475:2489	arg1	modulus					2464:2470	compressive Young's modulus	2444:2470	compressive Young's modulus	2444:2470	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56MPa, which was 37 times of that of the original hydrogel.					
35688402	1	102	theme	bone	210:213	arg1	engineering					222:232	bone tissue engineering	210:232	bone tissue engineering	210:232	Enzymatic mineralization has become an effective approach to enhancing the stiffness of hydrogels for bone tissue engineering, but generally with limited toughness.					
35688402	16	103	theme	mineralization	2760:2773	arg1	differentiation					2697:2711	osteogenic differentiation	2686:2711	osteogenic differentiation	2686:2711	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	16	103	theme	mineralization	2760:2773	arg1	proliferation					2658:2670	The cell proliferation	2649:2670	The cell proliferation	2649:2670	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	16	103	theme	mineralization	2760:2773	arg1	result					2740:2745	a result	2738:2745	a result	2738:2745	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	16	103	theme	mineralization	2760:2773	arg1	adhesion					2673:2680	adhesion	2673:2680	adhesion	2673:2680	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	6	104	theme	compressive	1054:1064	arg1	modulus					1066:1072	compressive modulus	1054:1072	compressive modulus	1054:1072	Synergistic effect of ionic cross-linking and enzymatic mineralization was clearly observed with dramatic increase in compressive modulus.					
35688402	0	105	theme	Enzymatic	82:90	arg1	Mineralization					92:105	Enzymatic Mineralization	82:105	Enzymatic Mineralization	82:105	Stiff and Tough Hydrogels Prepared Through Integration of Ionic Cross-linking and Enzymatic Mineralization.					
35688402	13	106	theme	tensile	2245:2251	arg1	stiffness					2253:2261	the compressive and tensile stiffness	2225:2261	the compressive and tensile stiffness of SA-PAM DN hydrogels	2225:2284	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	12	107	theme	tissue	2061:2066	arg1	applications					2080:2091	the bone tissue engineering applications	2052:2091	the bone tissue engineering applications	2052:2091	STATEMENT OF SIGNIFICANCE: Hydrogels with excellent stiffness, stability and biocompatibility have attracted significant attentions in the bone tissue engineering applications.					
35688402	2	108	with	hydrogel	330:337	arg1	toughness					353:361	enhanced toughness	344:361	enhanced toughness	344:361	On the other hand, double network cross-linking provides hydrogel with enhanced toughness.					
35688402	11	109	theme	bone	1879:1882	arg1	engineering					1891:1901	bone tissue engineering	1879:1901	bone tissue engineering	1879:1901	Our results provided an effective way for the preparation of stiff and tough hydrogels with osteogenesis, and demonstrated potential in bone tissue engineering applications.					
35688402	9	110	theme	Such	1376:1379	arg1	effect					1393:1398	Such synergistic effect	1376:1398	Such synergistic effect from Sr<sup>2+</sup>	1376:1419	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	1	111	theme	tissue	215:220	arg1	engineering					222:232	bone tissue engineering	210:232	bone tissue engineering	210:232	Enzymatic mineralization has become an effective approach to enhancing the stiffness of hydrogels for bone tissue engineering, but generally with limited toughness.					
35688402	4	112	dep	absence	746:752	arg1	the					742:744	the	742:744	the	742:744	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	7	113	theme	original	1247:1254	arg1	hydrogel					1256:1263	the original hydrogel	1243:1263	the original hydrogel	1243:1263	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56 MPa, which was 37 times of that of the original hydrogel.					
35688402	13	114	theme	compressive	2229:2239	arg1	stiffness					2253:2261	the compressive and tensile stiffness	2225:2261	the compressive and tensile stiffness of SA-PAM DN hydrogels	2225:2284	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	3	115	theme	cross-linking	400:412	arg1	method					414:419	double cross-linking method	393:419	double cross-linking method	393:419	In this study, we integrated double cross-linking method with enzymatic mineralization to synthesize stiff and tough hydrogels.					
35688402	14	116	theme	compressive	2444:2454	arg1	times					2505:2509	37 times	2502:2509	37 times of that of the original hydrogel	2502:2542	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56MPa, which was 37 times of that of the original hydrogel.					
35688402	14	116	theme	compressive	2444:2454	arg1	modulus					2464:2470	compressive Young's modulus	2444:2470	compressive Young's modulus	2444:2470	In particular, mineralized hydrogel cross-linked with Sr<sup>2+</sup> showed the highest compressive Young's modulus of 17.28 ± 3.56MPa, which was 37 times of that of the original hydrogel.					
35688402	1	117	theme	Enzymatic	108:116	arg1	mineralization					118:131	Enzymatic mineralization	108:131	Enzymatic mineralization	108:131	Enzymatic mineralization has become an effective approach to enhancing the stiffness of hydrogels for bone tissue engineering, but generally with limited toughness.					
35688402	11	118	theme	stiff	1804:1808	arg1	hydrogels					1820:1828	stiff and tough hydrogels	1804:1828	stiff and tough hydrogels with osteogenesis	1804:1846	Our results provided an effective way for the preparation of stiff and tough hydrogels with osteogenesis, and demonstrated potential in bone tissue engineering applications.					
35688402	5	119	theme	cross-linking	834:846	arg1	ions					848:851	specific cross-linking ions	825:851	specific cross-linking ions	825:851	In particular, we examined the role of specific cross-linking ions, i.e., calcium, zinc and strontium ions, in modulating the mineralization process.					
35688402	13	120	theme	enzymatic	2171:2179	arg1	mineralization					2181:2194	enzymatic mineralization	2171:2194	enzymatic mineralization	2171:2194	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	12	121	theme	SIGNIFICANCE	1930:1941	arg1	STATEMENT					1917:1925	STATEMENT	1917:1925	STATEMENT OF SIGNIFICANCE: Hydrogels with excellent stiffness, stability and biocompatibility have attracted significant attentions in the bone tissue engineering applications.	1917:2092	STATEMENT OF SIGNIFICANCE: Hydrogels with excellent stiffness, stability and biocompatibility have attracted significant attentions in the bone tissue engineering applications.					
35688402	16	122	theme	best	2862:2865	arg1	performance					2867:2877	the best performance	2858:2877	the best performance	2858:2877	The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the MSCs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.					
35688402	4	123	theme	hydrogels	582:590	arg1	kinds					518:522	three kinds	512:522	three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels	512:590	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	9	124	theme	uniformed	1446:1454	arg1	process					1471:1477	a more uniformed mineralization process	1439:1477	a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure	1439:1590	Such synergistic effect from Sr<sup>2+</sup> was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.					
35688402	1	125	with	stiffness	183:191	arg1	toughness					262:270	limited toughness	254:270	limited toughness	254:270	Enzymatic mineralization has become an effective approach to enhancing the stiffness of hydrogels for bone tissue engineering, but generally with limited toughness.					
35688402	4	126	theme	mechanical	663:672	arg1	properties					674:683	mechanical properties	663:683	mechanical properties	663:683	We have synthesized three kinds of sodium alginate-polyacrylamide (SA-PAM) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.					
35688402	11	127	theme	tough	1814:1818	arg1	hydrogels					1820:1828	stiff and tough hydrogels	1804:1828	stiff and tough hydrogels with osteogenesis	1804:1846	Our results provided an effective way for the preparation of stiff and tough hydrogels with osteogenesis, and demonstrated potential in bone tissue engineering applications.					
35688402	13	128	theme	cellular	2336:2343	arg1	response					2345:2352	cellular response	2336:2352	cellular response	2336:2352	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	13	129	theme	cross-linking	2153:2165	arg1	effect					2137:2142	the synergistic effect	2121:2142	the synergistic effect of ionic cross-linking and enzymatic mineralization	2121:2194	Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of SA-PAM DN hydrogels, as well as the toughness, swelling stability and cellular response.					
35688402	1	130	theme	limited	254:260	arg1	toughness					262:270	limited toughness	254:270	limited toughness	254:270	Enzymatic mineralization has become an effective approach to enhancing the stiffness of hydrogels for bone tissue engineering, but generally with limited toughness.					
37073751	5	0	theme	insignificant	664:676	arg1	changes					678:684	RESULTS insignificant changes	656:684	RESULTS insignificant changes	656:684	RESULTS insignificant changes were observed in pH values and acetic acid accumulated during fermentation.					
37073751	10	1	theme	prebiotic	1459:1467	arg1	activity					1469:1476	the prebiotic activity	1455:1476	the prebiotic activity of food ingredients	1455:1496	CONCLUSION despite the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients, it is not useful for detecting the prebiotic nature of Lf due to its nature as a protein.					
37073751	3	2	from	GOS	465:467	arg1	vessels					478:484	vessels	478:484	vessels of a small-scale batch culture fermentation model	478:534	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	9	3	theme	Enterococci	1343:1353	arg1	growth					1355:1360	Enterococci growth	1343:1360	Enterococci growth	1343:1360	After 24 hours of fermentation, all tested ingredients showed significant similarities in Enterococcus for controls except for 0.20 % rhLf + 1 % GOS, which provoked a diminution of Enterococci growth.					
37073751	0	4	theme	intestinal	74:83	arg1	microbiota					85:94	infant intestinal microbiota	67:94	infant intestinal microbiota	67:94	In vitro prebiotic activity of rhLf and galactooligosaccharides on infant intestinal microbiota.					
37073751	2	5	theme	composition	319:329	arg1	modulation					290:299	the modulation	286:299	the modulation of gut microbiota composition	286:329	So, the present study focusses on the role of Lf and/or galactooligosaccharides (GOS) in the modulation of gut microbiota composition.					
37073751	9	6	theme	growth	1355:1360	arg1	diminution					1329:1338	a diminution	1327:1338	a diminution of Enterococci growth	1327:1360	After 24 hours of fermentation, all tested ingredients showed significant similarities in Enterococcus for controls except for 0.20 % rhLf + 1 % GOS, which provoked a diminution of Enterococci growth.					
37073751	6	7	theme	butyric	826:832	arg1	acid					834:837	butyric acid	826:837	butyric acid	826:837	Propionic acid content has been insignificantly increased while butyric acid has been insignificantly decreased.					
37073751	11	8	from	activity	1624:1631	arg1	microbiota					1644:1653	the gut microbiota	1636:1653	the gut microbiota	1636:1653	Thus, Lf maybe shows its prebiotic activity on the gut microbiota through other mechanisms.					
37073751	10	9	theme	Lf	1554:1555	arg1	nature					1544:1549	the prebiotic nature	1530:1549	the prebiotic nature of Lf	1530:1555	CONCLUSION despite the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients, it is not useful for detecting the prebiotic nature of Lf due to its nature as a protein.					
37073751	3	10	theme	recombinant	340:350	arg1	rhLf					371:374	rhLf	371:374	rhLf	371:374	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	3	10	theme	recombinant	340:350	arg1	lactoferrin					358:368	recombinant human lactoferrin	340:368	METHODS recombinant human lactoferrin (rhLf)	332:375	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	1	11	contain	possess	162:168	arg2	range					177:181	a wide range	170:181	a wide range of functions	170:194	OBJECTIVE human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions.					
37073751	1	11	contain	possess	162:168	arg1	oligosaccharides					145:160	human milk oligosaccharides	134:160	human milk oligosaccharides	134:160	OBJECTIVE human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions.					
37073751	1	11	contain	possess	162:168	arg1	Lf					126:127	Lf	126:127	Lf	126:127	OBJECTIVE human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions.					
37073751	1	11	contain	possess	162:168	arg1	lactoferrin					113:123	OBJECTIVE human lactoferrin	97:123	OBJECTIVE human lactoferrin (Lf)	97:128	OBJECTIVE human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions.					
37073751	3	12	with	combination	448:458	arg1	GOS					465:467	GOS	465:467	GOS (1 %) in vessels of a small-scale batch culture fermentation model	465:534	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	3	12	with	combination	448:458	arg1	%					472:472	1 %	470:472	1 %	470:472	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	2	13	theme	galactooligosaccharides	253:275	arg1	role					235:238	the role	231:238	the role of Lf and/or galactooligosaccharides (GOS) in the modulation of gut microbiota composition	231:329	So, the present study focusses on the role of Lf and/or galactooligosaccharides (GOS) in the modulation of gut microbiota composition.					
37073751	11	14	theme	Lf	1595:1596	arg1	maybe					1598:1602	Lf maybe	1595:1602	Lf maybe	1595:1602	Thus, Lf maybe shows its prebiotic activity on the gut microbiota through other mechanisms.					
37073751	10	15	theme	fermentation	1418:1429	arg1	technique					1431:1439	the batch culture fermentation technique	1400:1439	the batch culture fermentation technique	1400:1439	CONCLUSION despite the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients, it is not useful for detecting the prebiotic nature of Lf due to its nature as a protein.					
37073751	3	16	theme	infant	400:405	arg1	%					433:433	0.10, 0.15, 0.20 %	416:433	0.10, 0.15, 0.20 %	416:433	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	3	16	theme	infant	400:405	arg1	formula					407:413	the first infant formula	390:413	the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model	390:534	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	8	17	theme	prebiotic	1121:1129	arg1	effect					1131:1136	the prebiotic effect	1117:1136	the prebiotic effect of lactoferrin and GOS	1117:1159	Lactobacillus and Bifidobacterium showed an increase in relation to initial time over the fermentation process, demonstrating the prebiotic effect of lactoferrin and GOS.					
37073751	10	18	theme	culture	1410:1416	arg1	technique					1431:1439	the batch culture fermentation technique	1400:1439	the batch culture fermentation technique	1400:1439	CONCLUSION despite the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients, it is not useful for detecting the prebiotic nature of Lf due to its nature as a protein.					
37073751	5	19	located	observed	691:698	arg2	changes					678:684	RESULTS insignificant changes	656:684	RESULTS insignificant changes	656:684	RESULTS insignificant changes were observed in pH values and acetic acid accumulated during fermentation.					
37073751	5	19	located	observed	691:698	arg1	values					706:711	pH values	703:711	pH values	703:711	RESULTS insignificant changes were observed in pH values and acetic acid accumulated during fermentation.					
37073751	2	20	theme	Lf	243:244	arg1	role					235:238	the role	231:238	the role of Lf and/or galactooligosaccharides (GOS) in the modulation of gut microbiota composition	231:329	So, the present study focusses on the role of Lf and/or galactooligosaccharides (GOS) in the modulation of gut microbiota composition.					
37073751	1	21	theme	wide	172:175	arg1	range					177:181	a wide range	170:181	a wide range of functions	170:194	OBJECTIVE human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions.					
37073751	8	22	theme	lactoferrin	1141:1151	arg1	effect					1131:1136	the prebiotic effect	1117:1136	the prebiotic effect of lactoferrin and GOS	1117:1159	Lactobacillus and Bifidobacterium showed an increase in relation to initial time over the fermentation process, demonstrating the prebiotic effect of lactoferrin and GOS.					
37073751	9	23	theme	0.20	1289:1292	arg1	%					1294:1294	%	1294:1294	%	1294:1294	After 24 hours of fermentation, all tested ingredients showed significant similarities in Enterococcus for controls except for 0.20 % rhLf + 1 % GOS, which provoked a diminution of Enterococci growth.					
37073751	10	24	theme	batch	1404:1408	arg1	technique					1431:1439	the batch culture fermentation technique	1400:1439	the batch culture fermentation technique	1400:1439	CONCLUSION despite the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients, it is not useful for detecting the prebiotic nature of Lf due to its nature as a protein.					
37073751	8	25	theme	fermentation	1081:1092	arg1	process					1094:1100	the fermentation process	1077:1100	the fermentation process	1077:1100	Lactobacillus and Bifidobacterium showed an increase in relation to initial time over the fermentation process, demonstrating the prebiotic effect of lactoferrin and GOS.					
37073751	10	26	theme	prebiotic	1534:1542	arg1	nature					1544:1549	the prebiotic nature	1530:1549	the prebiotic nature of Lf	1530:1555	CONCLUSION despite the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients, it is not useful for detecting the prebiotic nature of Lf due to its nature as a protein.					
37073751	4	27	theme	microbial	570:578	arg1	groups					591:596	microbial population groups	570:596	microbial population groups	570:596	Short-chain fatty acids (SCFAs), microbial population groups, and pH were monitored through fermentation for 24 hours.					
37073751	0	28	theme	In	0:1	arg1	activity					19:26	In vitro prebiotic activity	0:26	In vitro prebiotic activity of rhLf	0:34	In vitro prebiotic activity of rhLf and galactooligosaccharides on infant intestinal microbiota.					
37073751	9	29	theme	%	1294:1294	arg1	rhLf					1296:1299	0.20 % rhLf	1289:1299	0.20 % rhLf	1289:1299	After 24 hours of fermentation, all tested ingredients showed significant similarities in Enterococcus for controls except for 0.20 % rhLf + 1 % GOS, which provoked a diminution of Enterococci growth.					
37073751	10	30	theme	ingredients	1486:1496	arg1	activity					1469:1476	the prebiotic activity	1455:1476	the prebiotic activity of food ingredients	1455:1496	CONCLUSION despite the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients, it is not useful for detecting the prebiotic nature of Lf due to its nature as a protein.					
37073751	1	31	theme	OBJECTIVE	97:105	arg1	Lf					126:127	Lf	126:127	Lf	126:127	OBJECTIVE human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions.					
37073751	1	31	theme	OBJECTIVE	97:105	arg1	lactoferrin					113:123	OBJECTIVE human lactoferrin	97:123	OBJECTIVE human lactoferrin (Lf)	97:128	OBJECTIVE human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions.					
37073751	3	32	theme	first	394:398	arg1	%					433:433	0.10, 0.15, 0.20 %	416:433	0.10, 0.15, 0.20 %	416:433	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	3	32	theme	first	394:398	arg1	formula					407:413	the first infant formula	390:413	the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model	390:534	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	3	33	theme	human	352:356	arg1	rhLf					371:374	rhLf	371:374	rhLf	371:374	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	3	33	theme	human	352:356	arg1	lactoferrin					358:368	recombinant human lactoferrin	340:368	METHODS recombinant human lactoferrin (rhLf)	332:375	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	8	34	theme	GOS	1157:1159	arg1	effect					1131:1136	the prebiotic effect	1117:1136	the prebiotic effect of lactoferrin and GOS	1117:1159	Lactobacillus and Bifidobacterium showed an increase in relation to initial time over the fermentation process, demonstrating the prebiotic effect of lactoferrin and GOS.					
37073751	1	35	theme	human	107:111	arg1	Lf					126:127	Lf	126:127	Lf	126:127	OBJECTIVE human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions.					
37073751	1	35	theme	human	107:111	arg1	lactoferrin					113:123	OBJECTIVE human lactoferrin	97:123	OBJECTIVE human lactoferrin (Lf)	97:128	OBJECTIVE human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions.					
37073751	1	36	theme	functions	186:194	arg1	range					177:181	a wide range	170:181	a wide range of functions	170:194	OBJECTIVE human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions.					
37073751	0	37	theme	prebiotic	9:17	arg1	activity					19:26	In vitro prebiotic activity	0:26	In vitro prebiotic activity of rhLf	0:34	In vitro prebiotic activity of rhLf and galactooligosaccharides on infant intestinal microbiota.					
37073751	10	38	theme	food	1481:1484	arg1	ingredients					1486:1496	food ingredients	1481:1496	food ingredients	1481:1496	CONCLUSION despite the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients, it is not useful for detecting the prebiotic nature of Lf due to its nature as a protein.					
37073751	4	39	theme	Short-chain	537:547	arg1	acids					555:559	Short-chain fatty acids	537:559	Short-chain fatty acids (SCFAs)	537:567	Short-chain fatty acids (SCFAs), microbial population groups, and pH were monitored through fermentation for 24 hours.					
37073751	4	39	theme	Short-chain	537:547	arg1	SCFAs					562:566	SCFAs	562:566	SCFAs	562:566	Short-chain fatty acids (SCFAs), microbial population groups, and pH were monitored through fermentation for 24 hours.					
37073751	0	40	theme	rhLf	31:34	arg1	activity					19:26	In vitro prebiotic activity	0:26	In vitro prebiotic activity of rhLf	0:34	In vitro prebiotic activity of rhLf and galactooligosaccharides on infant intestinal microbiota.					
37073751	9	41	theme	1	1303:1303	arg1	%					1305:1305	%	1305:1305	%	1305:1305	After 24 hours of fermentation, all tested ingredients showed significant similarities in Enterococcus for controls except for 0.20 % rhLf + 1 % GOS, which provoked a diminution of Enterococci growth.					
37073751	11	42	theme	prebiotic	1614:1622	arg1	activity					1624:1631	its prebiotic activity	1610:1631	its prebiotic activity on the gut microbiota	1610:1653	Thus, Lf maybe shows its prebiotic activity on the gut microbiota through other mechanisms.					
37073751	9	43	theme	fermentation	1180:1191	arg1	hours					1171:1175	24 hours	1168:1175	24 hours of fermentation	1168:1191	After 24 hours of fermentation, all tested ingredients showed significant similarities in Enterococcus for controls except for 0.20 % rhLf + 1 % GOS, which provoked a diminution of Enterococci growth.					
37073751	3	44	theme	small-scale	491:501	arg1	model					530:534	a small-scale batch culture fermentation model	489:534	a small-scale batch culture fermentation model	489:534	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	2	45	from	role	235:238	arg1	modulation					290:299	the modulation	286:299	the modulation of gut microbiota composition	286:329	So, the present study focusses on the role of Lf and/or galactooligosaccharides (GOS) in the modulation of gut microbiota composition.					
37073751	7	46	from	increments	885:894	arg1	groups					913:918	all bacterial groups	899:918	all bacterial groups except for Bacteroides	899:941	Moreover, increments in all bacterial groups except for Bacteroides were observed through the fermentation process.					
37073751	10	47	theme	technique	1431:1439	arg1	importance					1386:1395	the importance	1382:1395	the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients	1382:1496	CONCLUSION despite the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients, it is not useful for detecting the prebiotic nature of Lf due to its nature as a protein.					
37073751	6	48	theme	acid	772:775	arg1	content					777:783	Propionic acid content	762:783	Propionic acid content	762:783	Propionic acid content has been insignificantly increased while butyric acid has been insignificantly decreased.					
37073751	3	49	theme	batch	503:507	arg1	model					530:534	a small-scale batch culture fermentation model	489:534	a small-scale batch culture fermentation model	489:534	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	5	50	theme	acetic	717:722	arg1	acid					724:727	acetic acid	717:727	acetic acid	717:727	RESULTS insignificant changes were observed in pH values and acetic acid accumulated during fermentation.					
37073751	9	51	from	similarities	1236:1247	arg1	Enterococcus					1252:1263	Enterococcus	1252:1263	Enterococcus	1252:1263	After 24 hours of fermentation, all tested ingredients showed significant similarities in Enterococcus for controls except for 0.20 % rhLf + 1 % GOS, which provoked a diminution of Enterococci growth.					
37073751	9	52	theme	%	1305:1305	arg1	GOS					1307:1309	1 % GOS	1303:1309	1 % GOS	1303:1309	After 24 hours of fermentation, all tested ingredients showed significant similarities in Enterococcus for controls except for 0.20 % rhLf + 1 % GOS, which provoked a diminution of Enterococci growth.					
37073751	8	53	theme	initial	1059:1065	arg1	time					1067:1070	initial time	1059:1070	initial time	1059:1070	Lactobacillus and Bifidobacterium showed an increase in relation to initial time over the fermentation process, demonstrating the prebiotic effect of lactoferrin and GOS.					
37073751	6	54	theme	Propionic	762:770	arg1	content					777:783	Propionic acid content	762:783	Propionic acid content	762:783	Propionic acid content has been insignificantly increased while butyric acid has been insignificantly decreased.					
37073751	2	55	theme	present	205:211	arg1	study					213:217	the present study	201:217	the present study	201:217	So, the present study focusses on the role of Lf and/or galactooligosaccharides (GOS) in the modulation of gut microbiota composition.					
37073751	2	56	theme	microbiota	308:317	arg1	composition					319:329	gut microbiota composition	304:329	gut microbiota composition	304:329	So, the present study focusses on the role of Lf and/or galactooligosaccharides (GOS) in the modulation of gut microbiota composition.					
37073751	5	57	theme	pH	703:704	arg1	values					706:711	pH values	703:711	pH values	703:711	RESULTS insignificant changes were observed in pH values and acetic acid accumulated during fermentation.					
37073751	9	58	theme	tested	1198:1203	arg1	ingredients					1205:1215	all tested ingredients	1194:1215	all tested ingredients	1194:1215	After 24 hours of fermentation, all tested ingredients showed significant similarities in Enterococcus for controls except for 0.20 % rhLf + 1 % GOS, which provoked a diminution of Enterococci growth.					
37073751	2	59	theme	gut	304:306	arg1	composition					319:329	gut microbiota composition	304:329	gut microbiota composition	304:329	So, the present study focusses on the role of Lf and/or galactooligosaccharides (GOS) in the modulation of gut microbiota composition.					
37073751	3	60	theme	fermentation	517:528	arg1	model					530:534	a small-scale batch culture fermentation model	489:534	a small-scale batch culture fermentation model	489:534	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	3	61	dep	METHODS	332:338	arg1	rhLf					371:374	rhLf	371:374	rhLf	371:374	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	3	61	dep	METHODS	332:338	arg1	lactoferrin					358:368	recombinant human lactoferrin	340:368	METHODS recombinant human lactoferrin (rhLf)	332:375	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	11	62	theme	gut	1640:1642	arg1	microbiota					1644:1653	the gut microbiota	1636:1653	the gut microbiota	1636:1653	Thus, Lf maybe shows its prebiotic activity on the gut microbiota through other mechanisms.					
37073751	3	63	theme	model	530:534	arg1	vessels					478:484	vessels	478:484	vessels of a small-scale batch culture fermentation model	478:534	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	11	64	theme	other	1663:1667	arg1	mechanisms					1669:1678	other mechanisms	1663:1678	other mechanisms	1663:1678	Thus, Lf maybe shows its prebiotic activity on the gut microbiota through other mechanisms.					
37073751	7	65	theme	fermentation	969:980	arg1	process					982:988	the fermentation process	965:988	the fermentation process	965:988	Moreover, increments in all bacterial groups except for Bacteroides were observed through the fermentation process.					
37073751	4	66	theme	population	580:589	arg1	groups					591:596	microbial population groups	570:596	microbial population groups	570:596	Short-chain fatty acids (SCFAs), microbial population groups, and pH were monitored through fermentation for 24 hours.					
37073751	10	67	dep	CONCLUSION	1363:1372	arg1	importance					1386:1395	the importance	1382:1395	the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients	1382:1496	CONCLUSION despite the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients, it is not useful for detecting the prebiotic nature of Lf due to its nature as a protein.					
37073751	0	68	theme	infant	67:72	arg1	microbiota					85:94	infant intestinal microbiota	67:94	infant intestinal microbiota	67:94	In vitro prebiotic activity of rhLf and galactooligosaccharides on infant intestinal microbiota.					
37073751	9	69	theme	significant	1224:1234	arg1	similarities					1236:1247	significant similarities	1224:1247	significant similarities in Enterococcus	1224:1263	After 24 hours of fermentation, all tested ingredients showed significant similarities in Enterococcus for controls except for 0.20 % rhLf + 1 % GOS, which provoked a diminution of Enterococci growth.					
37073751	3	70	theme	culture	509:515	arg1	model					530:534	a small-scale batch culture fermentation model	489:534	a small-scale batch culture fermentation model	489:534	METHODS recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model.					
37073751	4	71	theme	fatty	549:553	arg1	acids					555:559	Short-chain fatty acids	537:559	Short-chain fatty acids (SCFAs)	537:567	Short-chain fatty acids (SCFAs), microbial population groups, and pH were monitored through fermentation for 24 hours.					
37073751	4	71	theme	fatty	549:553	arg1	SCFAs					562:566	SCFAs	562:566	SCFAs	562:566	Short-chain fatty acids (SCFAs), microbial population groups, and pH were monitored through fermentation for 24 hours.					
37073751	1	72	theme	human	134:138	arg1	oligosaccharides					145:160	human milk oligosaccharides	134:160	human milk oligosaccharides	134:160	OBJECTIVE human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions.					
37073751	8	73	from	increase	1035:1042	arg1	relation					1047:1054	relation	1047:1054	relation to initial time	1047:1070	Lactobacillus and Bifidobacterium showed an increase in relation to initial time over the fermentation process, demonstrating the prebiotic effect of lactoferrin and GOS.					
37073751	5	74	theme	RESULTS	656:662	arg1	changes					678:684	RESULTS insignificant changes	656:684	RESULTS insignificant changes	656:684	RESULTS insignificant changes were observed in pH values and acetic acid accumulated during fermentation.					
37073751	0	75	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro prebiotic activity of rhLf and galactooligosaccharides on infant intestinal microbiota.					
37073751	7	76	theme	bacterial	903:911	arg1	groups					913:918	all bacterial groups	899:918	all bacterial groups except for Bacteroides	899:941	Moreover, increments in all bacterial groups except for Bacteroides were observed through the fermentation process.					
37073751	1	77	theme	milk	140:143	arg1	oligosaccharides					145:160	human milk oligosaccharides	134:160	human milk oligosaccharides	134:160	OBJECTIVE human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions.					
35905759	1	0	theme	biopolymer	287:296	arg1	matrix					298:303	a biopolymer matrix	285:303	a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA)	285:357	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	6	1	theme	anthocyanin	964:974	arg1	concentration					976:988	An increased anthocyanin concentration	951:988	An increased anthocyanin concentration on polymer films	951:1005	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	10	2	theme	room	1748:1751	arg1	temperature					1753:1763	room temperature	1748:1763	room temperature	1748:1763	The CPC smart films also showed promising application in terms of monitoring freshness of the fish fillets at room temperature.					
35905759	10	3	theme	fillets	1737:1743	arg1	freshness					1715:1723	freshness	1715:1723	freshness of the fish fillets at room temperature	1715:1763	The CPC smart films also showed promising application in terms of monitoring freshness of the fish fillets at room temperature.					
35905759	1	4	theme	natural	206:212	arg1	anthocyanin					214:224	the natural anthocyanin	202:224	the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC)	202:409	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	4	5	theme	matrix	717:722	arg1	miscibility					724:734	CS/PVA matrix miscibility	710:734	CS/PVA matrix miscibility	710:734	The smooth SEM images demonstrated CS/PVA matrix miscibility and compatibility with anthocyanin for the film formation.					
35905759	5	6	theme	films	931:935	arg1	transparency					937:948	the films transparency	927:948	the films transparency	927:948	The addition of anthocyanin to the CS/PVA films significantly reduced UV-Vis light transmission, while causing a slight decrease in the films transparency.					
35905759	3	7	theme	prepared	580:587	arg1	films					599:603	The prepared composite films	576:603	The prepared composite films	576:603	The prepared composite films are characterized by various analytical and spectroscopic techniques.					
35905759	1	8	theme	cuspidifolia	241:252	arg1	flower					259:264	cuspidifolia (JC) flower	241:264	cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA)	241:357	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	2	9	theme	conventional	555:566	arg1	method					568:573	the conventional method	551:573	the conventional method	551:573	We introduced microwave irradiation to prepare polymeric composite films noticed faster mixing of the polymers and extract take place than the conventional method.					
35905759	4	10	theme	SEM	686:688	arg1	images					690:695	The smooth SEM images	675:695	The smooth SEM images	675:695	The smooth SEM images demonstrated CS/PVA matrix miscibility and compatibility with anthocyanin for the film formation.					
35905759	11	11	theme	smart	1820:1824	arg1	films					1826:1830	the prepared CPC smart films	1803:1830	the prepared CPC smart films	1803:1830	The obtained results suggested that, the prepared CPC smart films have potential to be used as quality indicator in the marine food packaging system.					
35905759	0	12	theme	freshness	164:172	arg1	monitoring					145:154	real-time monitoring	135:154	real-time monitoring of fish freshness	135:172	Fabrication of intelligent/active films based on chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness.					
35905759	3	13	theme	various	626:632	arg1	techniques					663:672	various analytical and spectroscopic techniques	626:672	various analytical and spectroscopic techniques	626:672	The prepared composite films are characterized by various analytical and spectroscopic techniques.					
35905759	5	14	theme	slight	908:913	arg1	decrease					915:922	a slight decrease	906:922	a slight decrease in the films transparency	906:948	The addition of anthocyanin to the CS/PVA films significantly reduced UV-Vis light transmission, while causing a slight decrease in the films transparency.					
35905759	6	15	theme	improved	1014:1021	arg1	permeability					1030:1041	improved oxygen permeability	1014:1041	improved oxygen permeability (77.09 %)	1014:1051	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	6	15	theme	improved	1014:1021	arg1	%					1050:1050	77.09 %	1044:1050	77.09 %	1044:1050	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	11	16	theme	prepared	1807:1814	arg1	films					1826:1830	the prepared CPC smart films	1803:1830	the prepared CPC smart films	1803:1830	The obtained results suggested that, the prepared CPC smart films have potential to be used as quality indicator in the marine food packaging system.					
35905759	1	17	from	Jacaranda	231:239	arg1	anthocyanin					214:224	the natural anthocyanin	202:224	the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC)	202:409	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	4	18	theme	film	779:782	arg1	formation					784:792	the film formation	775:792	the film formation	775:792	The smooth SEM images demonstrated CS/PVA matrix miscibility and compatibility with anthocyanin for the film formation.					
35905759	10	19	from	temperature	1753:1763	arg1	freshness					1715:1723	freshness	1715:1723	freshness of the fish fillets at room temperature	1715:1763	The CPC smart films also showed promising application in terms of monitoring freshness of the fish fillets at room temperature.					
35905759	5	20	theme	anthocyanin	811:821	arg1	addition					799:806	The addition	795:806	The addition of anthocyanin to the CS/PVA films	795:841	The addition of anthocyanin to the CS/PVA films significantly reduced UV-Vis light transmission, while causing a slight decrease in the films transparency.					
35905759	7	21	theme	strong	1205:1210	arg1	antioxidant					1212:1222	strong antioxidant	1205:1222	strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli	1205:1334	Additionally, the prepared CPC smart films exhibited strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli.					
35905759	7	21	theme	strong	1205:1210	arg1	%					1231:1231	97.92 %	1225:1231	97.92 %	1225:1231	Additionally, the prepared CPC smart films exhibited strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli.					
35905759	5	22	from	decrease	915:922	arg1	transparency					937:948	the films transparency	927:948	the films transparency	927:948	The addition of anthocyanin to the CS/PVA films significantly reduced UV-Vis light transmission, while causing a slight decrease in the films transparency.					
35905759	1	23	theme	present	179:185	arg1	work					187:190	The present work	175:190	The present work	175:190	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	11	24	theme	obtained	1770:1777	arg1	results					1779:1785	The obtained results	1766:1785	The obtained results	1766:1785	The obtained results suggested that, the prepared CPC smart films have potential to be used as quality indicator in the marine food packaging system.					
35905759	0	25	theme	Jacaranda	96:104	arg1	anthocyanin					119:129	Jacaranda cuspidifolia anthocyanin	96:129	Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness	96:172	Fabrication of intelligent/active films based on chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness.					
35905759	9	26	theme	Total	1536:1540	arg1	content					1574:1580	the spoilage Total Volatile Basic-Nitrogen (TVB-N) content	1523:1580	the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period	1523:1635	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	6	27	theme	moisture	1054:1061	arg1	capacity					1073:1080	moisture retention capacity	1054:1080	moisture retention capacity (11.64 %)	1054:1090	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	6	27	theme	moisture	1054:1061	arg1	%					1089:1089	11.64 %	1083:1089	11.64 %	1083:1089	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	8	28	theme	smart	1363:1367	arg1	films					1369:1373	the prepared smart films	1350:1373	the prepared smart films	1350:1373	Furthermore, the prepared smart films demonstrated pink color in acidic, while grey to yellowish in basic solvent.					
35905759	1	29	theme	novel	364:368	arg1	CPC					406:408	CPC	406:408	CPC	406:408	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	1	29	theme	novel	364:368	arg1	films					399:403	novel intelligent/active packaging films	364:403	novel intelligent/active packaging films (CPC)	364:409	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	9	30	theme	Basic-Nitrogen	1551:1564	arg1	content					1574:1580	the spoilage Total Volatile Basic-Nitrogen (TVB-N) content	1523:1580	the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period	1523:1635	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	10	31	theme	promising	1670:1678	arg1	application					1680:1690	promising application	1670:1690	promising application in terms of monitoring freshness of the fish fillets at room temperature	1670:1763	The CPC smart films also showed promising application in terms of monitoring freshness of the fish fillets at room temperature.					
35905759	9	32	dep	freshness	1487:1495	arg1	label					1497:1501	label	1497:1501	label	1497:1501	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	11	33	theme	marine	1886:1891	arg1	system					1908:1913	the marine food packaging system	1882:1913	the marine food packaging system	1882:1913	The obtained results suggested that, the prepared CPC smart films have potential to be used as quality indicator in the marine food packaging system.					
35905759	0	34	theme	real-time	135:143	arg1	monitoring					145:154	real-time monitoring	135:154	real-time monitoring of fish freshness	135:172	Fabrication of intelligent/active films based on chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness.					
35905759	1	35	theme	packaging	389:397	arg1	CPC					406:408	CPC	406:408	CPC	406:408	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	1	35	theme	packaging	389:397	arg1	films					399:403	novel intelligent/active packaging films	364:403	novel intelligent/active packaging films (CPC)	364:409	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	5	36	theme	CS/PVA	830:835	arg1	films					837:841	the CS/PVA films	826:841	the CS/PVA films	826:841	The addition of anthocyanin to the CS/PVA films significantly reduced UV-Vis light transmission, while causing a slight decrease in the films transparency.					
35905759	9	37	theme	TVB-N	1567:1571	arg1	content					1574:1580	the spoilage Total Volatile Basic-Nitrogen (TVB-N) content	1523:1580	the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period	1523:1635	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	2	38	theme	composite	469:477	arg1	films					479:483	polymeric composite films	459:483	polymeric composite films noticed faster mixing of the polymers and extract take place than the conventional method	459:573	We introduced microwave irradiation to prepare polymeric composite films noticed faster mixing of the polymers and extract take place than the conventional method.					
35905759	2	38	theme	composite	469:477	arg1	mixing					500:505	mixing	500:505	mixing of the polymers and extract take place than the conventional method	500:573	We introduced microwave irradiation to prepare polymeric composite films noticed faster mixing of the polymers and extract take place than the conventional method.					
35905759	10	39	theme	CPC	1642:1644	arg1	films					1652:1656	The CPC smart films	1638:1656	The CPC smart films	1638:1656	The CPC smart films also showed promising application in terms of monitoring freshness of the fish fillets at room temperature.					
35905759	5	40	theme	light	872:876	arg1	transmission					878:889	UV-Vis light transmission	865:889	UV-Vis light transmission	865:889	The addition of anthocyanin to the CS/PVA films significantly reduced UV-Vis light transmission, while causing a slight decrease in the films transparency.					
35905759	6	41	theme	vapor	1103:1107	arg1	rate					1122:1125	water vapor transmission rate	1097:1125	water vapor transmission rate (43.10 %)	1097:1135	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	6	41	theme	vapor	1103:1107	arg1	%					1134:1134	43.10 %	1128:1134	43.10 %	1128:1134	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	0	42	theme	intelligent/active	15:32	arg1	films					34:38	intelligent/active films	15:38	intelligent/active films	15:38	Fabrication of intelligent/active films based on chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness.					
35905759	11	43	theme	packaging	1898:1906	arg1	system					1908:1913	the marine food packaging system	1882:1913	the marine food packaging system	1882:1913	The obtained results suggested that, the prepared CPC smart films have potential to be used as quality indicator in the marine food packaging system.					
35905759	9	44	theme	fish	1600:1603	arg1	samples					1605:1611	the fish samples	1596:1611	the fish samples with varied time period	1596:1635	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	0	45	theme	chitosan/polyvinyl	49:66	arg1	matrices					76:83	chitosan/polyvinyl alcohol matrices	49:83	chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness	49:172	Fabrication of intelligent/active films based on chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness.					
35905759	1	46	theme	polyvinyl	335:343	arg1	PVA					354:356	PVA	354:356	PVA	354:356	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	1	46	theme	polyvinyl	335:343	arg1	alcohol					345:351	polyvinyl alcohol	335:351	polyvinyl alcohol (PVA)	335:357	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	11	47	contain	have	1832:1835	arg2	potential					1837:1845	potential	1837:1845	potential	1837:1845	The obtained results suggested that, the prepared CPC smart films have potential to be used as quality indicator in the marine food packaging system.					
35905759	11	47	contain	have	1832:1835	arg1	films					1826:1830	the prepared CPC smart films	1803:1830	the prepared CPC smart films	1803:1830	The obtained results suggested that, the prepared CPC smart films have potential to be used as quality indicator in the marine food packaging system.					
35905759	2	48	dep	polymers	514:521	arg1	the					510:512	the	510:512	the	510:512	We introduced microwave irradiation to prepare polymeric composite films noticed faster mixing of the polymers and extract take place than the conventional method.					
35905759	9	49	theme	time	1625:1628	arg1	period					1630:1635	varied time period	1618:1635	varied time period	1618:1635	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	6	50	from	concentration	976:988	arg1	films					1001:1005	polymer films	993:1005	polymer films	993:1005	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	1	51	dep	Jacaranda	231:239	arg1	gave					359:362	gave	359:362	gave novel intelligent/active packaging films (CPC)	359:409	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	10	52	theme	fish	1732:1735	arg1	fillets					1737:1743	the fish fillets	1728:1743	the fish fillets	1728:1743	The CPC smart films also showed promising application in terms of monitoring freshness of the fish fillets at room temperature.					
35905759	0	53	theme	alcohol	68:74	arg1	matrices					76:83	chitosan/polyvinyl alcohol matrices	49:83	chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness	49:172	Fabrication of intelligent/active films based on chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness.					
35905759	6	54	theme	polymer	993:999	arg1	films					1001:1005	polymer films	993:1005	polymer films	993:1005	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	10	55	from	application	1680:1690	arg1	terms					1695:1699	terms	1695:1699	terms of monitoring freshness of the fish fillets at room temperature	1695:1763	The CPC smart films also showed promising application in terms of monitoring freshness of the fish fillets at room temperature.					
35905759	0	56	contain	containing	85:94	arg1	matrices					76:83	chitosan/polyvinyl alcohol matrices	49:83	chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness	49:172	Fabrication of intelligent/active films based on chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness.					
35905759	0	56	contain	containing	85:94	arg2	anthocyanin					119:129	Jacaranda cuspidifolia anthocyanin	96:129	Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness	96:172	Fabrication of intelligent/active films based on chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness.					
35905759	7	57	theme	common	1278:1283	arg1	coli					1331:1334	E. coli	1328:1334	E. coli	1328:1334	Additionally, the prepared CPC smart films exhibited strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli.					
35905759	7	57	theme	common	1278:1283	arg1	pathogens					1295:1303	common foodborne pathogens	1278:1303	common foodborne pathogens such as S. aureus, and E. coli	1278:1334	Additionally, the prepared CPC smart films exhibited strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli.					
35905759	7	57	theme	common	1278:1283	arg1	aureus					1316:1321	S. aureus	1313:1321	S. aureus	1313:1321	Additionally, the prepared CPC smart films exhibited strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli.					
35905759	9	58	with	samples	1605:1611	arg1	period					1630:1635	varied time period	1618:1635	varied time period	1618:1635	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	6	59	theme	increased	954:962	arg1	concentration					976:988	An increased anthocyanin concentration	951:988	An increased anthocyanin concentration on polymer films	951:1005	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	4	60	with	compatibility	740:752	arg1	anthocyanin					759:769	anthocyanin	759:769	anthocyanin	759:769	The smooth SEM images demonstrated CS/PVA matrix miscibility and compatibility with anthocyanin for the film formation.					
35905759	7	61	theme	CPC	1179:1181	arg1	films					1189:1193	the prepared CPC smart films	1166:1193	the prepared CPC smart films	1166:1193	Additionally, the prepared CPC smart films exhibited strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli.					
35905759	4	62	with	miscibility	724:734	arg1	anthocyanin					759:769	anthocyanin	759:769	anthocyanin	759:769	The smooth SEM images demonstrated CS/PVA matrix miscibility and compatibility with anthocyanin for the film formation.					
35905759	3	63	theme	composite	589:597	arg1	films					599:603	The prepared composite films	576:603	The prepared composite films	576:603	The prepared composite films are characterized by various analytical and spectroscopic techniques.					
35905759	4	64	theme	CS/PVA	710:715	arg1	miscibility					724:734	CS/PVA matrix miscibility	710:734	CS/PVA matrix miscibility	710:734	The smooth SEM images demonstrated CS/PVA matrix miscibility and compatibility with anthocyanin for the film formation.					
35905759	0	65	theme	fish	159:162	arg1	freshness					164:172	fish freshness	159:172	fish freshness	159:172	Fabrication of intelligent/active films based on chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness.					
35905759	9	66	theme	color	1465:1469	arg1	response					1471:1478	the color response	1461:1478	the color response of the freshness label	1461:1501	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	8	67	from	yellowish	1424:1432	arg1	solvent					1443:1449	basic solvent	1437:1449	basic solvent	1437:1449	Furthermore, the prepared smart films demonstrated pink color in acidic, while grey to yellowish in basic solvent.					
35905759	6	68	theme	oxygen	1023:1028	arg1	permeability					1030:1041	improved oxygen permeability	1014:1041	improved oxygen permeability (77.09 %)	1014:1051	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	6	68	theme	oxygen	1023:1028	arg1	%					1050:1050	77.09 %	1044:1050	77.09 %	1044:1050	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	4	69	theme	smooth	679:684	arg1	images					690:695	The smooth SEM images	675:695	The smooth SEM images	675:695	The smooth SEM images demonstrated CS/PVA matrix miscibility and compatibility with anthocyanin for the film formation.					
35905759	9	70	theme	freshness	1487:1495	arg1	response					1471:1478	the color response	1461:1478	the color response of the freshness label	1461:1501	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	11	71	theme	CPC	1816:1818	arg1	films					1826:1830	the prepared CPC smart films	1803:1830	the prepared CPC smart films	1803:1830	The obtained results suggested that, the prepared CPC smart films have potential to be used as quality indicator in the marine food packaging system.					
35905759	3	72	theme	spectroscopic	649:661	arg1	techniques					663:672	various analytical and spectroscopic techniques	626:672	various analytical and spectroscopic techniques	626:672	The prepared composite films are characterized by various analytical and spectroscopic techniques.					
35905759	7	73	theme	antibacterial	1245:1257	arg1	activities					1259:1268	antibacterial activities	1245:1268	strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli	1205:1334	Additionally, the prepared CPC smart films exhibited strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli.					
35905759	3	74	theme	analytical	634:643	arg1	techniques					663:672	various analytical and spectroscopic techniques	626:672	various analytical and spectroscopic techniques	626:672	The prepared composite films are characterized by various analytical and spectroscopic techniques.					
35905759	0	75	theme	cuspidifolia	106:117	arg1	anthocyanin					119:129	Jacaranda cuspidifolia anthocyanin	96:129	Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness	96:172	Fabrication of intelligent/active films based on chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness.					
35905759	2	76	dep	noticed	485:491	arg1	films					479:483	polymeric composite films	459:483	polymeric composite films noticed faster mixing of the polymers and extract take place than the conventional method	459:573	We introduced microwave irradiation to prepare polymeric composite films noticed faster mixing of the polymers and extract take place than the conventional method.					
35905759	2	76	dep	noticed	485:491	arg1	mixing					500:505	mixing	500:505	mixing of the polymers and extract take place than the conventional method	500:573	We introduced microwave irradiation to prepare polymeric composite films noticed faster mixing of the polymers and extract take place than the conventional method.					
35905759	9	77	theme	spoilage	1527:1534	arg1	content					1574:1580	the spoilage Total Volatile Basic-Nitrogen (TVB-N) content	1523:1580	the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period	1523:1635	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	6	78	theme	retention	1063:1071	arg1	capacity					1073:1080	moisture retention capacity	1054:1080	moisture retention capacity (11.64 %)	1054:1090	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	6	78	theme	retention	1063:1071	arg1	%					1089:1089	11.64 %	1083:1089	11.64 %	1083:1089	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	9	79	theme	Volatile	1542:1549	arg1	content					1574:1580	the spoilage Total Volatile Basic-Nitrogen (TVB-N) content	1523:1580	the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period	1523:1635	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	8	80	theme	prepared	1354:1361	arg1	films					1369:1373	the prepared smart films	1350:1373	the prepared smart films	1350:1373	Furthermore, the prepared smart films demonstrated pink color in acidic, while grey to yellowish in basic solvent.					
35905759	1	81	theme	intelligent/active	370:387	arg1	CPC					406:408	CPC	406:408	CPC	406:408	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	1	81	theme	intelligent/active	370:387	arg1	films					399:403	novel intelligent/active packaging films	364:403	novel intelligent/active packaging films (CPC)	364:409	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	9	82	with	consistent	1507:1516	arg1	content					1574:1580	the spoilage Total Volatile Basic-Nitrogen (TVB-N) content	1523:1580	the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period	1523:1635	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	10	83	theme	smart	1646:1650	arg1	films					1652:1656	The CPC smart films	1638:1656	The CPC smart films	1638:1656	The CPC smart films also showed promising application in terms of monitoring freshness of the fish fillets at room temperature.					
35905759	5	84	theme	UV-Vis	865:870	arg1	transmission					878:889	UV-Vis light transmission	865:889	UV-Vis light transmission	865:889	The addition of anthocyanin to the CS/PVA films significantly reduced UV-Vis light transmission, while causing a slight decrease in the films transparency.					
35905759	6	85	theme	transmission	1109:1120	arg1	rate					1122:1125	water vapor transmission rate	1097:1125	water vapor transmission rate (43.10 %)	1097:1135	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	6	85	theme	transmission	1109:1120	arg1	%					1134:1134	43.10 %	1128:1134	43.10 %	1128:1134	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	2	86	theme	polymeric	459:467	arg1	films					479:483	polymeric composite films	459:483	polymeric composite films noticed faster mixing of the polymers and extract take place than the conventional method	459:573	We introduced microwave irradiation to prepare polymeric composite films noticed faster mixing of the polymers and extract take place than the conventional method.					
35905759	2	86	theme	polymeric	459:467	arg1	mixing					500:505	mixing	500:505	mixing of the polymers and extract take place than the conventional method	500:573	We introduced microwave irradiation to prepare polymeric composite films noticed faster mixing of the polymers and extract take place than the conventional method.					
35905759	8	87	theme	pink	1388:1391	arg1	color					1393:1397	pink color	1388:1397	pink color	1388:1397	Furthermore, the prepared smart films demonstrated pink color in acidic, while grey to yellowish in basic solvent.					
35905759	0	88	theme	films	34:38	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of intelligent/active films	0:38	Fabrication of intelligent/active films based on chitosan/polyvinyl alcohol matrices containing Jacaranda cuspidifolia anthocyanin for real-time monitoring of fish freshness.					
35905759	11	89	from	indicator	1869:1877	arg1	system					1908:1913	the marine food packaging system	1882:1913	the marine food packaging system	1882:1913	The obtained results suggested that, the prepared CPC smart films have potential to be used as quality indicator in the marine food packaging system.					
35905759	6	90	theme	water	1097:1101	arg1	rate					1122:1125	water vapor transmission rate	1097:1125	water vapor transmission rate (43.10 %)	1097:1135	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	6	90	theme	water	1097:1101	arg1	%					1134:1134	43.10 %	1128:1134	43.10 %	1128:1134	An increased anthocyanin concentration on polymer films showed improved oxygen permeability (77.09 %), moisture retention capacity (11.64 %), and water vapor transmission rate (43.10 %) substantially.					
35905759	2	91	theme	microwave	426:434	arg1	irradiation					436:446	microwave irradiation	426:446	microwave irradiation	426:446	We introduced microwave irradiation to prepare polymeric composite films noticed faster mixing of the polymers and extract take place than the conventional method.					
35905759	7	92	theme	smart	1183:1187	arg1	films					1189:1193	the prepared CPC smart films	1166:1193	the prepared CPC smart films	1166:1193	Additionally, the prepared CPC smart films exhibited strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli.					
35905759	11	93	theme	quality	1861:1867	arg1	indicator					1869:1877	quality indicator	1861:1877	quality indicator in the marine food packaging system	1861:1913	The obtained results suggested that, the prepared CPC smart films have potential to be used as quality indicator in the marine food packaging system.					
35905759	7	94	theme	prepared	1170:1177	arg1	films					1189:1193	the prepared CPC smart films	1166:1193	the prepared CPC smart films	1166:1193	Additionally, the prepared CPC smart films exhibited strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli.					
35905759	9	95	theme	varied	1618:1623	arg1	period					1630:1635	varied time period	1618:1635	varied time period	1618:1635	Further, the color response of the freshness label was consistent with the spoilage Total Volatile Basic-Nitrogen (TVB-N) content determined in the fish samples with varied time period.					
35905759	1	96	theme	JC	255:256	arg1	flower					259:264	cuspidifolia (JC) flower	241:264	cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA)	241:357	The present work describes the natural anthocyanin from Jacaranda cuspidifolia (JC) flower immobilized within a biopolymer matrix composed of chitosan (CS) and polyvinyl alcohol (PVA) gave novel intelligent/active packaging films (CPC).					
35905759	7	97	theme	foodborne	1285:1293	arg1	coli					1331:1334	E. coli	1328:1334	E. coli	1328:1334	Additionally, the prepared CPC smart films exhibited strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli.					
35905759	7	97	theme	foodborne	1285:1293	arg1	pathogens					1295:1303	common foodborne pathogens	1278:1303	common foodborne pathogens such as S. aureus, and E. coli	1278:1334	Additionally, the prepared CPC smart films exhibited strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli.					
35905759	7	97	theme	foodborne	1285:1293	arg1	aureus					1316:1321	S. aureus	1313:1321	S. aureus	1313:1321	Additionally, the prepared CPC smart films exhibited strong antioxidant (97.92 %) as well as antibacterial activities against common foodborne pathogens such as S. aureus, and E. coli.					
35905759	11	98	theme	food	1893:1896	arg1	system					1908:1913	the marine food packaging system	1882:1913	the marine food packaging system	1882:1913	The obtained results suggested that, the prepared CPC smart films have potential to be used as quality indicator in the marine food packaging system.					
35905759	8	99	theme	basic	1437:1441	arg1	solvent					1443:1449	basic solvent	1437:1449	basic solvent	1437:1449	Furthermore, the prepared smart films demonstrated pink color in acidic, while grey to yellowish in basic solvent.					
35902014	9	0	theme	LCNPs	1369:1373	arg1	effects					1358:1364	the superior therapeutic effects	1333:1364	the superior therapeutic effects of LCNPs	1333:1373	The in vivo result over epidermal-infected wound demonstrates the superior therapeutic effects of LCNPs.					
35902014	8	1	theme	antibiotics	1175:1185	arg1	accumulation					1159:1170	the accumulation	1155:1170	the accumulation of antibiotics in the epidermis with retention ratio 2-3 fold higher than commercial formulations	1155:1268	Moreover, in vitro permeation studies demonstrate that LCNPs can increase the accumulation of antibiotics in the epidermis with retention ratio 2-3 fold higher than commercial formulations.					
35902014	0	2	theme	enhanced	98:105	arg1	therapy					107:113	enhanced therapy	98:113	enhanced therapy on epidermal bacterial infections	98:147	Efficient epidermal delivery of antibiotics by self-assembled lecithin/chitosan nanoparticles for enhanced therapy on epidermal bacterial infections.					
35902014	10	3	theme	bacterial	1500:1508	arg1	infections					1510:1519	epidermal bacterial infections	1490:1519	epidermal bacterial infections	1490:1519	The developed LCNPs represent an important advance in fabricating therapeutic materials for enhanced therapy over epidermal bacterial infections.					
35902014	2	4	theme	drug	395:398	arg1	delivery					400:407	efficient epidermal drug delivery	375:407	efficient epidermal drug delivery	375:407	Here we present a facile strategy to fabricate lecithin/chitosan nanoparticles (LCNPs) for efficient epidermal drug delivery over epidermal bacterial infections.					
35902014	0	5	theme	epidermal	118:126	arg1	infections					138:147	epidermal bacterial infections	118:147	epidermal bacterial infections	118:147	Efficient epidermal delivery of antibiotics by self-assembled lecithin/chitosan nanoparticles for enhanced therapy on epidermal bacterial infections.					
35902014	10	6	theme	epidermal	1490:1498	arg1	infections					1510:1519	epidermal bacterial infections	1490:1519	epidermal bacterial infections	1490:1519	The developed LCNPs represent an important advance in fabricating therapeutic materials for enhanced therapy over epidermal bacterial infections.					
35902014	9	7	dep	in	1275:1276	arg1	vivo					1278:1281	vivo	1278:1281	vivo	1278:1281	The in vivo result over epidermal-infected wound demonstrates the superior therapeutic effects of LCNPs.					
35902014	8	8	theme	ratio	1219:1223	arg1	fold					1229:1232	ratio 2-3 fold	1219:1232	ratio 2-3 fold	1219:1232	Moreover, in vitro permeation studies demonstrate that LCNPs can increase the accumulation of antibiotics in the epidermis with retention ratio 2-3 fold higher than commercial formulations.					
35902014	8	9	theme	in	1091:1092	arg1	studies					1111:1117	in vitro permeation studies	1091:1117	in vitro permeation studies	1091:1117	Moreover, in vitro permeation studies demonstrate that LCNPs can increase the accumulation of antibiotics in the epidermis with retention ratio 2-3 fold higher than commercial formulations.					
35902014	8	10	with	epidermis	1194:1202	arg1	retention					1209:1217	retention	1209:1217	retention ratio 2-3 fold higher than commercial formulations	1209:1268	Moreover, in vitro permeation studies demonstrate that LCNPs can increase the accumulation of antibiotics in the epidermis with retention ratio 2-3 fold higher than commercial formulations.					
35902014	8	11	from	accumulation	1159:1170	arg1	epidermis					1194:1202	the epidermis	1190:1202	the epidermis with retention ratio 2-3 fold higher than commercial formulations	1190:1268	Moreover, in vitro permeation studies demonstrate that LCNPs can increase the accumulation of antibiotics in the epidermis with retention ratio 2-3 fold higher than commercial formulations.					
35902014	4	12	theme	26.6 ± 1.2 mV	724:736	arg1	diameter					673:680	an average diameter	662:680	an average diameter of 325.9 ± 7.4 nm	662:698	The prepared CIP-LCNPs show an average diameter of 325.9 ± 7.4 nm and a zeta potential of 26.6 ± 1.2 mV.					
35902014	4	12	theme	26.6 ± 1.2 mV	724:736	arg1	potential					711:719	a zeta potential	704:719	a zeta potential of 26.6 ± 1.2 mV	704:736	The prepared CIP-LCNPs show an average diameter of 325.9 ± 7.4 nm and a zeta potential of 26.6 ± 1.2 mV.					
35902014	8	13	theme	higher	1234:1239	arg1	retention					1209:1217	retention	1209:1217	retention ratio 2-3 fold higher than commercial formulations	1209:1268	Moreover, in vitro permeation studies demonstrate that LCNPs can increase the accumulation of antibiotics in the epidermis with retention ratio 2-3 fold higher than commercial formulations.					
35902014	3	14	dep	and	542:544	arg1	obtained					591:598	obtained	591:598	was obtained via analysis of variance (ANOVA)	587:631	The central rotatable composite design method was used for the optimization of the preparation, and that the optimal size (212.63 ± 1.95 nm) was obtained via analysis of variance (ANOVA).					
35902014	4	15	theme	zeta	706:709	arg1	potential					711:719	a zeta potential	704:719	a zeta potential of 26.6 ± 1.2 mV	704:736	The prepared CIP-LCNPs show an average diameter of 325.9 ± 7.4 nm and a zeta potential of 26.6 ± 1.2 mV.					
35902014	0	16	theme	bacterial	128:136	arg1	infections					138:147	epidermal bacterial infections	118:147	epidermal bacterial infections	118:147	Efficient epidermal delivery of antibiotics by self-assembled lecithin/chitosan nanoparticles for enhanced therapy on epidermal bacterial infections.					
35902014	6	17	theme	skin	915:918	arg1	irritation					920:929	skin irritation	915:929	skin irritation	915:929	The hemolytic activity, cytotoxicity, and skin irritation are further investigated.					
35902014	8	18	theme	permeation	1100:1109	arg1	studies					1111:1117	in vitro permeation studies	1091:1117	in vitro permeation studies	1091:1117	Moreover, in vitro permeation studies demonstrate that LCNPs can increase the accumulation of antibiotics in the epidermis with retention ratio 2-3 fold higher than commercial formulations.					
35902014	7	19	theme	inhibition	970:979	arg1	zones					961:965	The zones	957:965	The zones of inhibition	957:979	The zones of inhibition are 2.16 ± 0.04 cm and 2.92 ± 0.03 cm for Escherichia coli and Staphylococcus aureus, respectively.					
35902014	7	19	theme	inhibition	970:979	arg1	2.16 ± 0.04 cm					985:998	2.16 ± 0.04 cm	985:998	2.16 ± 0.04 cm	985:998	The zones of inhibition are 2.16 ± 0.04 cm and 2.92 ± 0.03 cm for Escherichia coli and Staphylococcus aureus, respectively.					
35902014	2	20	theme	facile	302:307	arg1	strategy					309:316	a facile strategy	300:316	a facile strategy to fabricate lecithin/chitosan nanoparticles (LCNPs) for efficient epidermal drug delivery over epidermal bacterial infections	300:443	Here we present a facile strategy to fabricate lecithin/chitosan nanoparticles (LCNPs) for efficient epidermal drug delivery over epidermal bacterial infections.					
35902014	10	21	theme	therapeutic	1442:1452	arg1	materials					1454:1462	therapeutic materials	1442:1462	therapeutic materials for enhanced therapy	1442:1483	The developed LCNPs represent an important advance in fabricating therapeutic materials for enhanced therapy over epidermal bacterial infections.					
35902014	4	22	theme	325.9 ± 7.4 nm	685:698	arg1	diameter					673:680	an average diameter	662:680	an average diameter of 325.9 ± 7.4 nm	662:698	The prepared CIP-LCNPs show an average diameter of 325.9 ± 7.4 nm and a zeta potential of 26.6 ± 1.2 mV.					
35902014	4	22	theme	325.9 ± 7.4 nm	685:698	arg1	potential					711:719	a zeta potential	704:719	a zeta potential of 26.6 ± 1.2 mV	704:736	The prepared CIP-LCNPs show an average diameter of 325.9 ± 7.4 nm and a zeta potential of 26.6 ± 1.2 mV.					
35902014	0	23	theme	epidermal	10:18	arg1	delivery					20:27	Efficient epidermal delivery	0:27	Efficient epidermal delivery of antibiotics by self-assembled lecithin/chitosan	0:78	Efficient epidermal delivery of antibiotics by self-assembled lecithin/chitosan nanoparticles for enhanced therapy on epidermal bacterial infections.					
35902014	9	24	theme	in	1275:1276	arg1	result					1283:1288	The in vivo result	1271:1288	The in vivo result over epidermal-infected wound	1271:1318	The in vivo result over epidermal-infected wound demonstrates the superior therapeutic effects of LCNPs.					
35902014	3	25	theme	rotatable	458:466	arg1	method					485:490	The central rotatable composite design method	446:490	The central rotatable composite design method	446:490	The central rotatable composite design method was used for the optimization of the preparation, and that the optimal size (212.63 ± 1.95 nm) was obtained via analysis of variance (ANOVA).					
35902014	1	26	theme	primary	212:218	arg1	concern					228:234	a primary healthy concern	210:234	a primary healthy concern	210:234	The treatment for epidermal bacterial infections has become a primary healthy concern, producing a significant therapeutic challenge.					
35902014	0	27	theme	Efficient	0:8	arg1	delivery					20:27	Efficient epidermal delivery	0:27	Efficient epidermal delivery of antibiotics by self-assembled lecithin/chitosan	0:78	Efficient epidermal delivery of antibiotics by self-assembled lecithin/chitosan nanoparticles for enhanced therapy on epidermal bacterial infections.					
35902014	3	28	theme	variance	616:623	arg1	analysis					604:611	analysis	604:611	analysis of variance (ANOVA)	604:631	The central rotatable composite design method was used for the optimization of the preparation, and that the optimal size (212.63 ± 1.95 nm) was obtained via analysis of variance (ANOVA).					
35902014	2	29	theme	bacterial	424:432	arg1	infections					434:443	epidermal bacterial infections	414:443	epidermal bacterial infections	414:443	Here we present a facile strategy to fabricate lecithin/chitosan nanoparticles (LCNPs) for efficient epidermal drug delivery over epidermal bacterial infections.					
35902014	5	30	theme	%	861:861	arg1	release					837:843	cumulative release	826:843	cumulative release of 93.81 ± 2.05 % for 48 h	826:870	Antibiotics can be well encapsulated in LCNPs and its release kinetics is studied with cumulative release of 93.81 ± 2.05 % for 48 h.					
35902014	5	31	theme	cumulative	826:835	arg1	release					837:843	cumulative release	826:843	cumulative release of 93.81 ± 2.05 % for 48 h	826:870	Antibiotics can be well encapsulated in LCNPs and its release kinetics is studied with cumulative release of 93.81 ± 2.05 % for 48 h.					
35902014	10	32	dep	important	1409:1417	arg1	advance					1419:1425	advance	1419:1425	advance	1419:1425	The developed LCNPs represent an important advance in fabricating therapeutic materials for enhanced therapy over epidermal bacterial infections.					
35902014	4	33	theme	average	665:671	arg1	diameter					673:680	an average diameter	662:680	an average diameter of 325.9 ± 7.4 nm	662:698	The prepared CIP-LCNPs show an average diameter of 325.9 ± 7.4 nm and a zeta potential of 26.6 ± 1.2 mV.					
35902014	4	34	theme	prepared	638:645	arg1	CIP-LCNPs					647:655	The prepared CIP-LCNPs	634:655	The prepared CIP-LCNPs	634:655	The prepared CIP-LCNPs show an average diameter of 325.9 ± 7.4 nm and a zeta potential of 26.6 ± 1.2 mV.					
35902014	0	35	from	therapy	107:113	arg1	infections					138:147	epidermal bacterial infections	118:147	epidermal bacterial infections	118:147	Efficient epidermal delivery of antibiotics by self-assembled lecithin/chitosan nanoparticles for enhanced therapy on epidermal bacterial infections.					
35902014	2	36	theme	lecithin/chitosan	331:347	arg1	nanoparticles					349:361	lecithin/chitosan nanoparticles	331:361	lecithin/chitosan nanoparticles (LCNPs) for efficient epidermal drug delivery	331:407	Here we present a facile strategy to fabricate lecithin/chitosan nanoparticles (LCNPs) for efficient epidermal drug delivery over epidermal bacterial infections.					
35902014	2	36	theme	lecithin/chitosan	331:347	arg1	LCNPs					364:368	LCNPs	364:368	LCNPs	364:368	Here we present a facile strategy to fabricate lecithin/chitosan nanoparticles (LCNPs) for efficient epidermal drug delivery over epidermal bacterial infections.					
35902014	1	37	theme	epidermal	168:176	arg1	infections					188:197	epidermal bacterial infections	168:197	epidermal bacterial infections	168:197	The treatment for epidermal bacterial infections has become a primary healthy concern, producing a significant therapeutic challenge.					
35902014	3	38	theme	composite	468:476	arg1	method					485:490	The central rotatable composite design method	446:490	The central rotatable composite design method	446:490	The central rotatable composite design method was used for the optimization of the preparation, and that the optimal size (212.63 ± 1.95 nm) was obtained via analysis of variance (ANOVA).					
35902014	9	39	theme	epidermal-infected	1295:1312	arg1	wound					1314:1318	epidermal-infected wound	1295:1318	epidermal-infected wound	1295:1318	The in vivo result over epidermal-infected wound demonstrates the superior therapeutic effects of LCNPs.					
35902014	1	40	theme	healthy	220:226	arg1	concern					228:234	a primary healthy concern	210:234	a primary healthy concern	210:234	The treatment for epidermal bacterial infections has become a primary healthy concern, producing a significant therapeutic challenge.					
35902014	0	41	theme	antibiotics	32:42	arg1	delivery					20:27	Efficient epidermal delivery	0:27	Efficient epidermal delivery of antibiotics by self-assembled lecithin/chitosan	0:78	Efficient epidermal delivery of antibiotics by self-assembled lecithin/chitosan nanoparticles for enhanced therapy on epidermal bacterial infections.					
35902014	3	42	theme	design	478:483	arg1	method					485:490	The central rotatable composite design method	446:490	The central rotatable composite design method	446:490	The central rotatable composite design method was used for the optimization of the preparation, and that the optimal size (212.63 ± 1.95 nm) was obtained via analysis of variance (ANOVA).					
35902014	8	43	theme	commercial	1246:1255	arg1	formulations					1257:1268	commercial formulations	1246:1268	commercial formulations	1246:1268	Moreover, in vitro permeation studies demonstrate that LCNPs can increase the accumulation of antibiotics in the epidermis with retention ratio 2-3 fold higher than commercial formulations.					
35902014	3	44	theme	central	450:456	arg1	method					485:490	The central rotatable composite design method	446:490	The central rotatable composite design method	446:490	The central rotatable composite design method was used for the optimization of the preparation, and that the optimal size (212.63 ± 1.95 nm) was obtained via analysis of variance (ANOVA).					
35902014	8	45	theme	2-3	1225:1227	arg1	fold					1229:1232	ratio 2-3 fold	1219:1232	ratio 2-3 fold	1219:1232	Moreover, in vitro permeation studies demonstrate that LCNPs can increase the accumulation of antibiotics in the epidermis with retention ratio 2-3 fold higher than commercial formulations.					
35902014	10	46	theme	developed	1380:1388	arg1	LCNPs					1390:1394	The developed LCNPs	1376:1394	The developed LCNPs	1376:1394	The developed LCNPs represent an important advance in fabricating therapeutic materials for enhanced therapy over epidermal bacterial infections.					
35902014	3	47	dep	used	496:499	arg1	and					542:544	and	542:544	and	542:544	The central rotatable composite design method was used for the optimization of the preparation, and that the optimal size (212.63 ± 1.95 nm) was obtained via analysis of variance (ANOVA).					
35902014	2	48	theme	epidermal	385:393	arg1	delivery					400:407	efficient epidermal drug delivery	375:407	efficient epidermal drug delivery	375:407	Here we present a facile strategy to fabricate lecithin/chitosan nanoparticles (LCNPs) for efficient epidermal drug delivery over epidermal bacterial infections.					
35902014	0	49	theme	self-assembled	47:60	arg1	lecithin/chitosan					62:78	self-assembled lecithin/chitosan	47:78	self-assembled lecithin/chitosan	47:78	Efficient epidermal delivery of antibiotics by self-assembled lecithin/chitosan nanoparticles for enhanced therapy on epidermal bacterial infections.					
35902014	6	50	theme	hemolytic	877:885	arg1	activity					887:894	The hemolytic activity	873:894	The hemolytic activity	873:894	The hemolytic activity, cytotoxicity, and skin irritation are further investigated.					
35902014	3	51	theme	optimal	555:561	arg1	212.63 ± 1.95 nm					569:584	212.63 ± 1.95 nm	569:584	212.63 ± 1.95 nm	569:584	The central rotatable composite design method was used for the optimization of the preparation, and that the optimal size (212.63 ± 1.95 nm) was obtained via analysis of variance (ANOVA).					
35902014	3	51	theme	optimal	555:561	arg1	size					563:566	the optimal size	551:566	the optimal size (212.63 ± 1.95 nm)	551:585	The central rotatable composite design method was used for the optimization of the preparation, and that the optimal size (212.63 ± 1.95 nm) was obtained via analysis of variance (ANOVA).					
35902014	2	52	theme	efficient	375:383	arg1	delivery					400:407	efficient epidermal drug delivery	375:407	efficient epidermal drug delivery	375:407	Here we present a facile strategy to fabricate lecithin/chitosan nanoparticles (LCNPs) for efficient epidermal drug delivery over epidermal bacterial infections.					
35902014	9	53	theme	superior	1337:1344	arg1	effects					1358:1364	the superior therapeutic effects	1333:1364	the superior therapeutic effects of LCNPs	1333:1373	The in vivo result over epidermal-infected wound demonstrates the superior therapeutic effects of LCNPs.					
35902014	3	54	theme	preparation	529:539	arg1	optimization					509:520	the optimization	505:520	the optimization of the preparation	505:539	The central rotatable composite design method was used for the optimization of the preparation, and that the optimal size (212.63 ± 1.95 nm) was obtained via analysis of variance (ANOVA).					
35902014	1	55	theme	significant	249:259	arg1	challenge					273:281	a significant therapeutic challenge	247:281	a significant therapeutic challenge	247:281	The treatment for epidermal bacterial infections has become a primary healthy concern, producing a significant therapeutic challenge.					
35902014	3	56	used	used	496:499	arg2	method					485:490	The central rotatable composite design method	446:490	The central rotatable composite design method	446:490	The central rotatable composite design method was used for the optimization of the preparation, and that the optimal size (212.63 ± 1.95 nm) was obtained via analysis of variance (ANOVA).					
35902014	9	57	theme	therapeutic	1346:1356	arg1	effects					1358:1364	the superior therapeutic effects	1333:1364	the superior therapeutic effects of LCNPs	1333:1373	The in vivo result over epidermal-infected wound demonstrates the superior therapeutic effects of LCNPs.					
35902014	10	58	theme	enhanced	1468:1475	arg1	therapy					1477:1483	enhanced therapy	1468:1483	enhanced therapy	1468:1483	The developed LCNPs represent an important advance in fabricating therapeutic materials for enhanced therapy over epidermal bacterial infections.					
35902014	1	59	theme	therapeutic	261:271	arg1	challenge					273:281	a significant therapeutic challenge	247:281	a significant therapeutic challenge	247:281	The treatment for epidermal bacterial infections has become a primary healthy concern, producing a significant therapeutic challenge.					
35902014	1	60	dep	concern	228:234	arg1	producing					237:245	producing	237:245	producing a significant therapeutic challenge	237:281	The treatment for epidermal bacterial infections has become a primary healthy concern, producing a significant therapeutic challenge.					
35902014	2	61	theme	epidermal	414:422	arg1	infections					434:443	epidermal bacterial infections	414:443	epidermal bacterial infections	414:443	Here we present a facile strategy to fabricate lecithin/chitosan nanoparticles (LCNPs) for efficient epidermal drug delivery over epidermal bacterial infections.					
35902014	5	62	theme	release	793:799	arg1	kinetics					801:808	its release kinetics	789:808	its release kinetics	789:808	Antibiotics can be well encapsulated in LCNPs and its release kinetics is studied with cumulative release of 93.81 ± 2.05 % for 48 h.					
35902014	1	63	theme	bacterial	178:186	arg1	infections					188:197	epidermal bacterial infections	168:197	epidermal bacterial infections	168:197	The treatment for epidermal bacterial infections has become a primary healthy concern, producing a significant therapeutic challenge.					
35902014	8	64	dep	in	1091:1092	arg1	vitro					1094:1098	vitro	1094:1098	vitro	1094:1098	Moreover, in vitro permeation studies demonstrate that LCNPs can increase the accumulation of antibiotics in the epidermis with retention ratio 2-3 fold higher than commercial formulations.					
35358544	0	0	theme	layered	77:83	arg1	biosorbent					102:111	chitosan impregnated Ni-Fe layered double hydroxide biosorbent	50:111	chitosan impregnated Ni-Fe layered double hydroxide biosorbent	50:111	Ultrasonication expedited As(III) adsorption onto chitosan impregnated Ni-Fe layered double hydroxide biosorbent: Optimization studies and artificial intelligence modelling.					
35358544	3	1	theme	concentration	704:716	arg1	50 mgL-1					718:725	initial arsenic concentration 50 mgL-1	688:725	initial arsenic concentration 50 mgL-1	688:725	Maximal As(III) removal was obtained at adsorbent dose 1 gL-1, pH 7, ultrasonication time 30 min, temperature 298 K, and initial arsenic concentration 50 mgL-1.					
35358544	6	2	theme	consecutive	1300:1310	arg1	cycles					1322:1327	5 consecutive treatment cycles	1298:1327	5 consecutive treatment cycles	1298:1327	The prepared material successfully brought down arsenic level by 62% in a natural water sample and showed good reusability up to 5 consecutive treatment cycles.					
35358544	2	3	theme	X-ray	364:368	arg1	diffraction					370:380	X-ray diffraction	364:380	X-ray diffraction	364:380	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	0	4	theme	Ni-Fe	71:75	arg1	biosorbent					102:111	chitosan impregnated Ni-Fe layered double hydroxide biosorbent	50:111	chitosan impregnated Ni-Fe layered double hydroxide biosorbent	50:111	Ultrasonication expedited As(III) adsorption onto chitosan impregnated Ni-Fe layered double hydroxide biosorbent: Optimization studies and artificial intelligence modelling.					
35358544	4	5	theme	spontaneous	896:906	arg1	reaction					908:915	spontaneous reaction	896:915	spontaneous reaction (ΔG = -8.13 kJ mol-1)	896:937	The experimentally obtained values fit the Langmuir isotherm and pseudo-second-order dynamics well (R2 > 0.98), while thermodynamic evaluation confirmed exothermic and spontaneous reaction (ΔG = -8.13 kJ mol-1).					
35358544	4	5	theme	spontaneous	896:906	arg1	ΔG = -8.13 kJ mol-1					918:936	ΔG = -8.13 kJ mol-1	918:936	ΔG = -8.13 kJ mol-1	918:936	The experimentally obtained values fit the Langmuir isotherm and pseudo-second-order dynamics well (R2 > 0.98), while thermodynamic evaluation confirmed exothermic and spontaneous reaction (ΔG = -8.13 kJ mol-1).					
35358544	1	6	theme	Chitosan	174:181	arg1	hydroxide					217:225	Chitosan intercalated Ni-Fe layered double hydroxide	174:225	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch)	174:240	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch), prepared by co-precipitation was examined for adsorptive elimination of arsenic (III).					
35358544	1	6	theme	Chitosan	174:181	arg1	LDH/Ch					234:239	LDH/Ch	234:239	LDH/Ch	234:239	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch), prepared by co-precipitation was examined for adsorptive elimination of arsenic (III).					
35358544	0	7	theme	hydroxide	92:100	arg1	biosorbent					102:111	chitosan impregnated Ni-Fe layered double hydroxide biosorbent	50:111	chitosan impregnated Ni-Fe layered double hydroxide biosorbent	50:111	Ultrasonication expedited As(III) adsorption onto chitosan impregnated Ni-Fe layered double hydroxide biosorbent: Optimization studies and artificial intelligence modelling.					
35358544	3	8	theme	initial	688:694	arg1	50 mgL-1					718:725	initial arsenic concentration 50 mgL-1	688:725	initial arsenic concentration 50 mgL-1	688:725	Maximal As(III) removal was obtained at adsorbent dose 1 gL-1, pH 7, ultrasonication time 30 min, temperature 298 K, and initial arsenic concentration 50 mgL-1.					
35358544	5	9	theme	high	1074:1077	arg1	R2 > 0.94					1104:1112	R2 > 0.94	1104:1112	R2 > 0.94	1104:1112	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	5	9	theme	high	1074:1077	arg1	coefficient					1091:1101	a high correlation coefficient	1072:1101	a high correlation coefficient (R2 > 0.94)	1072:1113	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	3	10	theme	As	575:576	arg1	removal					583:589	Maximal As(III) removal	567:589	Maximal As(III) removal	567:589	Maximal As(III) removal was obtained at adsorbent dose 1 gL-1, pH 7, ultrasonication time 30 min, temperature 298 K, and initial arsenic concentration 50 mgL-1.					
35358544	6	11	from	%	1236:1236	arg1	sample					1257:1262	a natural water sample	1241:1262	a natural water sample	1241:1262	The prepared material successfully brought down arsenic level by 62% in a natural water sample and showed good reusability up to 5 consecutive treatment cycles.					
35358544	0	12	theme	double	85:90	arg1	biosorbent					102:111	chitosan impregnated Ni-Fe layered double hydroxide biosorbent	50:111	chitosan impregnated Ni-Fe layered double hydroxide biosorbent	50:111	Ultrasonication expedited As(III) adsorption onto chitosan impregnated Ni-Fe layered double hydroxide biosorbent: Optimization studies and artificial intelligence modelling.					
35358544	4	13	theme	Langmuir	771:778	arg1	isotherm					780:787	the Langmuir isotherm	767:787	the Langmuir isotherm	767:787	The experimentally obtained values fit the Langmuir isotherm and pseudo-second-order dynamics well (R2 > 0.98), while thermodynamic evaluation confirmed exothermic and spontaneous reaction (ΔG = -8.13 kJ mol-1).					
35358544	3	14	dep	removal	583:589	arg1	III					578:580	III	578:580	III	578:580	Maximal As(III) removal was obtained at adsorbent dose 1 gL-1, pH 7, ultrasonication time 30 min, temperature 298 K, and initial arsenic concentration 50 mgL-1.					
35358544	2	15	theme	enhanced	540:547	arg1	sites					560:564	enhanced adsorption sites	540:564	enhanced adsorption sites	540:564	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	5	16	theme	artificial	991:1000	arg1	network					1009:1015	artificial neural network	991:1015	artificial neural network	991:1015	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	5	17	dep	percentage	1056:1065	arg1	III					1043:1045	III	1043:1045	III	1043:1045	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	4	18	theme	pseudo-second-order	793:811	arg1	dynamics					813:820	pseudo-second-order dynamics	793:820	pseudo-second-order dynamics	793:820	The experimentally obtained values fit the Langmuir isotherm and pseudo-second-order dynamics well (R2 > 0.98), while thermodynamic evaluation confirmed exothermic and spontaneous reaction (ΔG = -8.13 kJ mol-1).					
35358544	4	19	dep	well	822:825	arg1	R2 > 0.98					828:836	R2 > 0.98	828:836	R2 > 0.98	828:836	The experimentally obtained values fit the Langmuir isotherm and pseudo-second-order dynamics well (R2 > 0.98), while thermodynamic evaluation confirmed exothermic and spontaneous reaction (ΔG = -8.13 kJ mol-1).					
35358544	1	20	theme	intercalated	183:194	arg1	hydroxide					217:225	Chitosan intercalated Ni-Fe layered double hydroxide	174:225	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch)	174:240	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch), prepared by co-precipitation was examined for adsorptive elimination of arsenic (III).					
35358544	1	20	theme	intercalated	183:194	arg1	LDH/Ch					234:239	LDH/Ch	234:239	LDH/Ch	234:239	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch), prepared by co-precipitation was examined for adsorptive elimination of arsenic (III).					
35358544	7	21	theme	large-scale	1467:1477	arg1	applications					1479:1490	large-scale applications	1467:1490	large-scale applications	1467:1490	The results recommended that Ni-Fe LDH/Ch has ample potential for arsenic remediation, and further investigations can be carried out for large-scale applications.					
35358544	6	22	theme	good	1275:1278	arg1	reusability					1280:1290	good reusability	1275:1290	good reusability up to 5 consecutive treatment cycles	1275:1327	The prepared material successfully brought down arsenic level by 62% in a natural water sample and showed good reusability up to 5 consecutive treatment cycles.					
35358544	3	23	theme	Maximal	567:573	arg1	removal					583:589	Maximal As(III) removal	567:589	Maximal As(III) removal	567:589	Maximal As(III) removal was obtained at adsorbent dose 1 gL-1, pH 7, ultrasonication time 30 min, temperature 298 K, and initial arsenic concentration 50 mgL-1.					
35358544	1	24	theme	Ni-Fe	196:200	arg1	hydroxide					217:225	Chitosan intercalated Ni-Fe layered double hydroxide	174:225	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch)	174:240	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch), prepared by co-precipitation was examined for adsorptive elimination of arsenic (III).					
35358544	1	24	theme	Ni-Fe	196:200	arg1	LDH/Ch					234:239	LDH/Ch	234:239	LDH/Ch	234:239	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch), prepared by co-precipitation was examined for adsorptive elimination of arsenic (III).					
35358544	2	25	theme	Light	466:470	arg1	Scattering					472:481	Dynamic Light Scattering	458:481	Dynamic Light Scattering	458:481	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	2	26	theme	composite	525:533	arg1	synthesis					508:516	the successful synthesis	493:516	the successful synthesis of the composite	493:533	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	6	27	theme	water	1251:1255	arg1	sample					1257:1262	a natural water sample	1241:1262	a natural water sample	1241:1262	The prepared material successfully brought down arsenic level by 62% in a natural water sample and showed good reusability up to 5 consecutive treatment cycles.					
35358544	1	28	theme	layered	202:208	arg1	hydroxide					217:225	Chitosan intercalated Ni-Fe layered double hydroxide	174:225	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch)	174:240	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch), prepared by co-precipitation was examined for adsorptive elimination of arsenic (III).					
35358544	1	28	theme	layered	202:208	arg1	LDH/Ch					234:239	LDH/Ch	234:239	LDH/Ch	234:239	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch), prepared by co-precipitation was examined for adsorptive elimination of arsenic (III).					
35358544	2	29	theme	Dynamic	458:464	arg1	Scattering					472:481	Dynamic Light Scattering	458:481	Dynamic Light Scattering	458:481	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	1	30	theme	adsorptive	289:298	arg1	elimination					300:310	adsorptive elimination	289:310	adsorptive elimination of arsenic (III)	289:327	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch), prepared by co-precipitation was examined for adsorptive elimination of arsenic (III).					
35358544	4	31	theme	obtained	747:754	arg1	values					756:761	The experimentally obtained values	728:761	The experimentally obtained values	728:761	The experimentally obtained values fit the Langmuir isotherm and pseudo-second-order dynamics well (R2 > 0.98), while thermodynamic evaluation confirmed exothermic and spontaneous reaction (ΔG = -8.13 kJ mol-1).					
35358544	1	32	theme	double	210:215	arg1	hydroxide					217:225	Chitosan intercalated Ni-Fe layered double hydroxide	174:225	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch)	174:240	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch), prepared by co-precipitation was examined for adsorptive elimination of arsenic (III).					
35358544	1	32	theme	double	210:215	arg1	LDH/Ch					234:239	LDH/Ch	234:239	LDH/Ch	234:239	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch), prepared by co-precipitation was examined for adsorptive elimination of arsenic (III).					
35358544	7	33	theme	arsenic	1396:1402	arg1	remediation					1404:1414	arsenic remediation	1396:1414	arsenic remediation	1396:1414	The results recommended that Ni-Fe LDH/Ch has ample potential for arsenic remediation, and further investigations can be carried out for large-scale applications.					
35358544	5	34	theme	neural	1002:1007	arg1	network					1009:1015	artificial neural network	991:1015	artificial neural network	991:1015	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	5	35	theme	neuro-fuzzy	958:968	arg1	system					980:985	adaptive neuro-fuzzy inference system	949:985	adaptive neuro-fuzzy inference system	949:985	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	0	36	theme	Optimization	114:125	arg1	studies					127:133	Optimization studies	114:133	Optimization studies	114:133	Ultrasonication expedited As(III) adsorption onto chitosan impregnated Ni-Fe layered double hydroxide biosorbent: Optimization studies and artificial intelligence modelling.					
35358544	3	37	theme	time	652:655	arg1	30 min					657:662	ultrasonication time 30 min	636:662	ultrasonication time 30 min	636:662	Maximal As(III) removal was obtained at adsorbent dose 1 gL-1, pH 7, ultrasonication time 30 min, temperature 298 K, and initial arsenic concentration 50 mgL-1.					
35358544	7	38	theme	further	1421:1427	arg1	investigations					1429:1442	further investigations	1421:1442	further investigations	1421:1442	The results recommended that Ni-Fe LDH/Ch has ample potential for arsenic remediation, and further investigations can be carried out for large-scale applications.					
35358544	2	39	theme	Electron	433:440	arg1	Microscopy					442:451	Scanning Electron Microscopy	424:451	Scanning Electron Microscopy	424:451	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	2	40	theme	successful	497:506	arg1	synthesis					508:516	the successful synthesis	493:516	the successful synthesis of the composite	493:533	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	1	41	theme	arsenic	315:321	arg1	elimination					300:310	adsorptive elimination	289:310	adsorptive elimination of arsenic (III)	289:327	Chitosan intercalated Ni-Fe layered double hydroxide (Ni-Fe LDH/Ch), prepared by co-precipitation was examined for adsorptive elimination of arsenic (III).					
35358544	0	42	theme	artificial	139:148	arg1	modelling					163:171	artificial intelligence modelling	139:171	artificial intelligence modelling	139:171	Ultrasonication expedited As(III) adsorption onto chitosan impregnated Ni-Fe layered double hydroxide biosorbent: Optimization studies and artificial intelligence modelling.					
35358544	5	43	theme	low	1119:1121	arg1	errors					1135:1140	low statistical errors	1119:1140	low statistical errors (MSE< 0.002, AARE< 0.063)	1119:1166	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	3	44	theme	adsorbent	607:615	arg1	1 gL-1					622:627	adsorbent dose 1 gL-1	607:627	adsorbent dose 1 gL-1	607:627	Maximal As(III) removal was obtained at adsorbent dose 1 gL-1, pH 7, ultrasonication time 30 min, temperature 298 K, and initial arsenic concentration 50 mgL-1.					
35358544	2	45	theme	Scanning	424:431	arg1	Microscopy					442:451	Scanning Electron Microscopy	424:451	Scanning Electron Microscopy	424:451	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	5	46	theme	statistical	1123:1133	arg1	errors					1135:1140	low statistical errors	1119:1140	low statistical errors (MSE< 0.002, AARE< 0.063)	1119:1166	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	0	47	dep	adsorption	34:43	arg1	III					29:31	III	29:31	III	29:31	Ultrasonication expedited As(III) adsorption onto chitosan impregnated Ni-Fe layered double hydroxide biosorbent: Optimization studies and artificial intelligence modelling.					
35358544	3	48	theme	ultrasonication	636:650	arg1	30 min					657:662	ultrasonication time 30 min	636:662	ultrasonication time 30 min	636:662	Maximal As(III) removal was obtained at adsorbent dose 1 gL-1, pH 7, ultrasonication time 30 min, temperature 298 K, and initial arsenic concentration 50 mgL-1.					
35358544	4	49	theme	thermodynamic	846:858	arg1	evaluation					860:869	thermodynamic evaluation	846:869	thermodynamic evaluation	846:869	The experimentally obtained values fit the Langmuir isotherm and pseudo-second-order dynamics well (R2 > 0.98), while thermodynamic evaluation confirmed exothermic and spontaneous reaction (ΔG = -8.13 kJ mol-1).					
35358544	5	50	theme	adaptive	949:956	arg1	system					980:985	adaptive neuro-fuzzy inference system	949:985	adaptive neuro-fuzzy inference system	949:985	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	6	51	theme	arsenic	1217:1223	arg1	level					1225:1229	down arsenic level	1212:1229	down arsenic level	1212:1229	The prepared material successfully brought down arsenic level by 62% in a natural water sample and showed good reusability up to 5 consecutive treatment cycles.					
35358544	0	52	theme	intelligence	150:161	arg1	modelling					163:171	artificial intelligence modelling	139:171	artificial intelligence modelling	139:171	Ultrasonication expedited As(III) adsorption onto chitosan impregnated Ni-Fe layered double hydroxide biosorbent: Optimization studies and artificial intelligence modelling.					
35358544	5	53	theme	correlation	1079:1089	arg1	R2 > 0.94					1104:1112	R2 > 0.94	1104:1112	R2 > 0.94	1104:1112	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	5	53	theme	correlation	1079:1089	arg1	coefficient					1091:1101	a high correlation coefficient	1072:1101	a high correlation coefficient (R2 > 0.94)	1072:1113	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	7	54	theme	Ni-Fe	1359:1363	arg1	LDH/Ch					1365:1370	Ni-Fe LDH/Ch	1359:1370	Ni-Fe LDH/Ch	1359:1370	The results recommended that Ni-Fe LDH/Ch has ample potential for arsenic remediation, and further investigations can be carried out for large-scale applications.					
35358544	2	55	theme	X-ray	348:352	arg1	analysis					354:361	Energy Dispersive X-ray analysis	330:361	Energy Dispersive X-ray analysis	330:361	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	5	56	with	percentage	1056:1065	arg1	R2 > 0.94					1104:1112	R2 > 0.94	1104:1112	R2 > 0.94	1104:1112	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	5	56	with	percentage	1056:1065	arg1	coefficient					1091:1101	a high correlation coefficient	1072:1101	a high correlation coefficient (R2 > 0.94)	1072:1113	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	5	56	with	percentage	1056:1065	arg1	errors					1135:1140	low statistical errors	1119:1140	low statistical errors (MSE< 0.002, AARE< 0.063)	1119:1166	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	6	57	theme	down	1212:1215	arg1	level					1225:1229	down arsenic level	1212:1229	down arsenic level	1212:1229	The prepared material successfully brought down arsenic level by 62% in a natural water sample and showed good reusability up to 5 consecutive treatment cycles.					
35358544	7	58	theme	ample	1376:1380	arg1	potential					1382:1390	ample potential	1376:1390	ample potential for arsenic remediation	1376:1414	The results recommended that Ni-Fe LDH/Ch has ample potential for arsenic remediation, and further investigations can be carried out for large-scale applications.					
35358544	3	59	theme	dose	617:620	arg1	1 gL-1					622:627	adsorbent dose 1 gL-1	607:627	adsorbent dose 1 gL-1	607:627	Maximal As(III) removal was obtained at adsorbent dose 1 gL-1, pH 7, ultrasonication time 30 min, temperature 298 K, and initial arsenic concentration 50 mgL-1.					
35358544	2	60	theme	Dispersive	337:346	arg1	analysis					354:361	Energy Dispersive X-ray analysis	330:361	Energy Dispersive X-ray analysis	330:361	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	6	61	theme	prepared	1173:1180	arg1	material					1182:1189	The prepared material	1169:1189	The prepared material	1169:1189	The prepared material successfully brought down arsenic level by 62% in a natural water sample and showed good reusability up to 5 consecutive treatment cycles.					
35358544	7	62	contain	has	1372:1374	arg1	LDH/Ch					1365:1370	Ni-Fe LDH/Ch	1359:1370	Ni-Fe LDH/Ch	1359:1370	The results recommended that Ni-Fe LDH/Ch has ample potential for arsenic remediation, and further investigations can be carried out for large-scale applications.					
35358544	7	62	contain	has	1372:1374	arg2	potential					1382:1390	ample potential	1376:1390	ample potential for arsenic remediation	1376:1414	The results recommended that Ni-Fe LDH/Ch has ample potential for arsenic remediation, and further investigations can be carried out for large-scale applications.					
35358544	6	63	theme	treatment	1312:1320	arg1	cycles					1322:1327	5 consecutive treatment cycles	1298:1327	5 consecutive treatment cycles	1298:1327	The prepared material successfully brought down arsenic level by 62% in a natural water sample and showed good reusability up to 5 consecutive treatment cycles.					
35358544	6	64	theme	natural	1243:1249	arg1	sample					1257:1262	a natural water sample	1241:1262	a natural water sample	1241:1262	The prepared material successfully brought down arsenic level by 62% in a natural water sample and showed good reusability up to 5 consecutive treatment cycles.					
35358544	2	65	dep	Fourier	383:389	arg1	Transform					391:399	Transform	391:399	Transform Infrared spectroscopy	391:421	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	2	66	theme	Energy	330:335	arg1	analysis					354:361	Energy Dispersive X-ray analysis	330:361	Energy Dispersive X-ray analysis	330:361	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	5	67	theme	inference	970:978	arg1	system					980:985	adaptive neuro-fuzzy inference system	949:985	adaptive neuro-fuzzy inference system	949:985	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	0	68	theme	impregnated	59:69	arg1	biosorbent					102:111	chitosan impregnated Ni-Fe layered double hydroxide biosorbent	50:111	chitosan impregnated Ni-Fe layered double hydroxide biosorbent	50:111	Ultrasonication expedited As(III) adsorption onto chitosan impregnated Ni-Fe layered double hydroxide biosorbent: Optimization studies and artificial intelligence modelling.					
35358544	3	69	theme	temperature	665:675	arg1	298 K					677:681	temperature 298 K	665:681	temperature 298 K	665:681	Maximal As(III) removal was obtained at adsorbent dose 1 gL-1, pH 7, ultrasonication time 30 min, temperature 298 K, and initial arsenic concentration 50 mgL-1.					
35358544	5	70	theme	removal	1048:1054	arg1	percentage					1056:1065	(III) removal percentage	1042:1065	(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063)	1042:1166	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	2	71	theme	Infrared	401:408	arg1	spectroscopy					410:421	Infrared spectroscopy	401:421	Infrared spectroscopy	401:421	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
35358544	0	72	theme	chitosan	50:57	arg1	biosorbent					102:111	chitosan impregnated Ni-Fe layered double hydroxide biosorbent	50:111	chitosan impregnated Ni-Fe layered double hydroxide biosorbent	50:111	Ultrasonication expedited As(III) adsorption onto chitosan impregnated Ni-Fe layered double hydroxide biosorbent: Optimization studies and artificial intelligence modelling.					
35358544	3	73	theme	arsenic	696:702	arg1	50 mgL-1					718:725	initial arsenic concentration 50 mgL-1	688:725	initial arsenic concentration 50 mgL-1	688:725	Maximal As(III) removal was obtained at adsorbent dose 1 gL-1, pH 7, ultrasonication time 30 min, temperature 298 K, and initial arsenic concentration 50 mgL-1.					
35358544	5	74	dep	errors	1135:1140	arg1	MSE<					1143:1146	MSE<	1143:1146	MSE<	1143:1146	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	5	74	dep	errors	1135:1140	arg1	AARE<					1155:1159	AARE<	1155:1159	AARE<	1155:1159	Further, adaptive neuro-fuzzy inference system and artificial neural network successfully predicted As(III) removal percentage with a high correlation coefficient (R2 > 0.94) and low statistical errors (MSE< 0.002, AARE< 0.063).					
35358544	2	75	theme	adsorption	549:558	arg1	sites					560:564	enhanced adsorption sites	540:564	enhanced adsorption sites	540:564	Energy Dispersive X-ray analysis, X-ray diffraction, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, and Dynamic Light Scattering validated the successful synthesis of the composite with enhanced adsorption sites.					
37195240	10	0	theme	third	1232:1236	arg1	phases					1238:1243	the second and third phases	1217:1243	the second and third phases	1217:1243	In the second and third phases, higher energy consumption occurred in the OF group than in the UF and AF groups.					
37195240	4	1	theme	LPS	597:599	arg1	rats					601:604	LPS rats	597:604	LPS rats	597:604	METHODS Rats were categorized into the lipopolysaccharide (LPS) received or control groups; LPS rats were categorized into underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups.					
37195240	13	2	theme	calorie	1584:1590	arg1	intake					1592:1597	calorie intake	1584:1597	calorie intake	1584:1597	CONCLUSIONS We observed metabolic changes with IDC during the acute systemic inflammation phase owing to differences in calorie intake.					
37195240	10	3	theme	second	1221:1226	arg1	phases					1238:1243	the second and third phases	1217:1243	the second and third phases	1217:1243	In the second and third phases, higher energy consumption occurred in the OF group than in the UF and AF groups.					
37195240	4	4	theme	adjusted	647:654	arg1	AF					665:666	AF	665:666	AF	665:666	METHODS Rats were categorized into the lipopolysaccharide (LPS) received or control groups; LPS rats were categorized into underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups.					
37195240	4	4	theme	adjusted	647:654	arg1	feeding					656:662	adjusted feeding	647:662	adjusted feeding (AF)	647:667	METHODS Rats were categorized into the lipopolysaccharide (LPS) received or control groups; LPS rats were categorized into underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups.					
37195240	7	5	theme	control	1006:1012	arg1	group					1014:1018	the control group	1002:1018	the control group	1002:1018	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	10	6	theme	energy	1253:1258	arg1	consumption					1260:1270	higher energy consumption	1246:1270	higher energy consumption	1246:1270	In the second and third phases, higher energy consumption occurred in the OF group than in the UF and AF groups.					
37195240	1	7	theme	ill	204:206	arg1	patients					208:215	severely ill patients	195:215	severely ill patients	195:215	BACKGROUND Nutritional management is crucial for severely ill patients.					
37195240	6	8	theme	tissue	813:818	arg1	weight					820:825	tissue weight	813:825	tissue weight	813:825	Body composition was measured at -24 and 72 or 144 hours and tissue weight was measured at 72 or 144 hours.					
37195240	8	9	from	REE	1077:1079	arg1	the					1084:1086	the	1084:1086	the	1084:1086	The REE in the OF group was higher than that in the UF and AF groups.					
37195240	14	10	theme	rat	1698:1700	arg1	model					1702:1706	the LPS-induced systemic inflammation rat model	1660:1706	the LPS-induced systemic inflammation rat model	1660:1706	This is the first report of long-term IDC measurement using the LPS-induced systemic inflammation rat model.					
37195240	12	11	theme	weight	1417:1422	arg1	loss					1424:1427	body weight loss	1412:1427	body weight loss	1412:1427	Muscle atrophy caused body weight loss but fat tissue loss did not occur.					
37195240	13	12	theme	acute	1526:1530	arg1	phase					1554:1558	the acute systemic inflammation phase	1522:1558	the acute systemic inflammation phase owing to differences in calorie intake	1522:1597	CONCLUSIONS We observed metabolic changes with IDC during the acute systemic inflammation phase owing to differences in calorie intake.					
37195240	3	13	with	patients	468:475	arg1	inflammation					491:502	systemic inflammation	482:502	systemic inflammation	482:502	Indirect calorimetry (IDC) is assumed to be useful for acute intensive care; however, there are few studies on long-term IDC measurement in patients with systemic inflammation.					
37195240	7	14	theme	expenditure	939:949	arg1	variation					911:919	diurnal variation	903:919	diurnal variation of resting energy expenditure (REE)	903:955	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	14	15	theme	systemic	1676:1683	arg1	model					1702:1706	the LPS-induced systemic inflammation rat model	1660:1706	the LPS-induced systemic inflammation rat model	1660:1706	This is the first report of long-term IDC measurement using the LPS-induced systemic inflammation rat model.					
37195240	13	16	dep	CONCLUSIONS	1464:1474	arg1	observed					1479:1486	observed	1479:1486	observed metabolic changes with IDC during the acute systemic inflammation phase owing to differences in calorie intake	1479:1597	CONCLUSIONS We observed metabolic changes with IDC during the acute systemic inflammation phase owing to differences in calorie intake.					
37195240	13	17	theme	inflammation	1541:1552	arg1	phase					1554:1558	the acute systemic inflammation phase	1522:1558	the acute systemic inflammation phase owing to differences in calorie intake	1522:1597	CONCLUSIONS We observed metabolic changes with IDC during the acute systemic inflammation phase owing to differences in calorie intake.					
37195240	7	18	theme	resting	924:930	arg1	REE					952:954	REE	952:954	REE	952:954	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	7	18	theme	resting	924:930	arg1	expenditure					939:949	resting energy expenditure	924:949	resting energy expenditure (REE)	924:955	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	12	19	theme	Muscle	1390:1395	arg1	atrophy					1397:1403	Muscle atrophy	1390:1403	Muscle atrophy	1390:1403	Muscle atrophy caused body weight loss but fat tissue loss did not occur.					
37195240	9	20	located	observed	1190:1197	arg2	consumption					1174:1184	low energy consumption	1163:1184	low energy consumption	1163:1184	In the first phase, low energy consumption was observed in all groups.					
37195240	9	20	located	observed	1190:1197	arg1	phase					1156:1160	the first phase	1146:1160	the first phase	1146:1160	In the first phase, low energy consumption was observed in all groups.					
37195240	9	20	located	observed	1190:1197	arg1	groups					1206:1211	all groups	1202:1211	all groups	1202:1211	In the first phase, low energy consumption was observed in all groups.					
37195240	2	21	theme	sepsis	281:286	arg1	phase					288:292	the acute sepsis phase	271:292	the acute sepsis phase	271:292	Measuring metabolism is believed to be necessary for the acute sepsis phase to accurately estimate nutrition.					
37195240	7	22	theme	variation	911:919	arg1	consumption					879:889	Low energy consumption	868:889	Low energy consumption	868:889	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	7	22	theme	variation	911:919	arg1	loss					895:898	loss	895:898	loss of diurnal variation of resting energy expenditure (REE)	895:955	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	1	23	theme	Nutritional	157:167	arg1	management					169:178	BACKGROUND Nutritional management	146:178	BACKGROUND Nutritional management	146:178	BACKGROUND Nutritional management is crucial for severely ill patients.					
37195240	0	24	theme	inflammation	62:73	arg1	model					79:83	an LPS-induced systemic inflammation rat model	38:83	an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments	38:143	Dynamic metabolic changes observed in an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments.					
37195240	14	25	theme	long-term	1628:1636	arg1	measurement					1642:1652	long-term IDC measurement	1628:1652	long-term IDC measurement using the LPS-induced systemic inflammation rat model	1628:1706	This is the first report of long-term IDC measurement using the LPS-induced systemic inflammation rat model.					
37195240	4	26	theme	METHODS	505:511	arg1	Rats					513:516	METHODS Rats	505:516	METHODS Rats	505:516	METHODS Rats were categorized into the lipopolysaccharide (LPS) received or control groups; LPS rats were categorized into underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups.					
37195240	13	27	with	changes	1498:1504	arg1	IDC					1511:1513	IDC	1511:1513	IDC	1511:1513	CONCLUSIONS We observed metabolic changes with IDC during the acute systemic inflammation phase owing to differences in calorie intake.					
37195240	5	28	theme	72	735:736	arg1	hours					745:749	72 or 144 hours	735:749	hours	745:749	IDC measurement was performed until 72 or 144 hours.					
37195240	11	29	theme	third	1334:1338	arg1	phase					1340:1344	the third phase	1330:1344	the third phase	1330:1344	In the third phase, diurnal variation recovered in all groups.					
37195240	0	30	theme	continuous	91:100	arg1	experiments					133:143	continuous long-term indirect calorimetry experiments	91:143	continuous long-term indirect calorimetry experiments	91:143	Dynamic metabolic changes observed in an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments.					
37195240	7	31	theme	energy	872:877	arg1	consumption					879:889	Low energy consumption	868:889	Low energy consumption	868:889	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	14	32	theme	measurement	1642:1652	arg1	report					1618:1623	the first report	1608:1623	the first report of long-term IDC measurement using the LPS-induced systemic inflammation rat model	1608:1706	This is the first report of long-term IDC measurement using the LPS-induced systemic inflammation rat model.					
37195240	14	32	theme	measurement	1642:1652	arg1	This					1600:1603	This	1600:1603	This	1600:1603	This is the first report of long-term IDC measurement using the LPS-induced systemic inflammation rat model.					
37195240	0	33	theme	indirect	112:119	arg1	experiments					133:143	continuous long-term indirect calorimetry experiments	91:143	continuous long-term indirect calorimetry experiments	91:143	Dynamic metabolic changes observed in an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments.					
37195240	9	34	theme	low	1163:1165	arg1	consumption					1174:1184	low energy consumption	1163:1184	low energy consumption	1163:1184	In the first phase, low energy consumption was observed in all groups.					
37195240	3	35	theme	few	424:426	arg1	studies					428:434	few studies	424:434	few studies on long-term IDC measurement in patients with systemic inflammation	424:502	Indirect calorimetry (IDC) is assumed to be useful for acute intensive care; however, there are few studies on long-term IDC measurement in patients with systemic inflammation.					
37195240	0	36	theme	Dynamic	0:6	arg1	changes					18:24	Dynamic metabolic changes	0:24	Dynamic metabolic changes	0:24	Dynamic metabolic changes observed in an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments.					
37195240	14	37	theme	first	1612:1616	arg1	report					1618:1623	the first report	1608:1623	the first report of long-term IDC measurement using the LPS-induced systemic inflammation rat model	1608:1706	This is the first report of long-term IDC measurement using the LPS-induced systemic inflammation rat model.					
37195240	14	37	theme	first	1612:1616	arg1	This					1600:1603	This	1600:1603	This	1600:1603	This is the first report of long-term IDC measurement using the LPS-induced systemic inflammation rat model.					
37195240	12	38	theme	fat	1433:1435	arg1	loss					1444:1447	fat tissue loss	1433:1447	fat tissue loss	1433:1447	Muscle atrophy caused body weight loss but fat tissue loss did not occur.					
37195240	3	39	theme	acute	383:387	arg1	care					399:402	acute intensive care	383:402	acute intensive care	383:402	Indirect calorimetry (IDC) is assumed to be useful for acute intensive care; however, there are few studies on long-term IDC measurement in patients with systemic inflammation.					
37195240	10	40	theme	AF	1316:1317	arg1	groups					1319:1324	the UF and AF groups	1305:1324	the UF and AF groups	1305:1324	In the second and third phases, higher energy consumption occurred in the OF group than in the UF and AF groups.					
37195240	4	41	dep	overfeeding	674:684	arg1	OF					687:688	OF	687:688	OF	687:688	METHODS Rats were categorized into the lipopolysaccharide (LPS) received or control groups; LPS rats were categorized into underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups.					
37195240	4	42	theme	received	569:576	arg1	groups					589:594	the lipopolysaccharide (LPS) received or control groups	540:594	the lipopolysaccharide (LPS) received or control groups	540:594	METHODS Rats were categorized into the lipopolysaccharide (LPS) received or control groups; LPS rats were categorized into underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups.					
37195240	10	43	theme	UF	1309:1310	arg1	groups					1319:1324	the UF and AF groups	1305:1324	the UF and AF groups	1305:1324	In the second and third phases, higher energy consumption occurred in the OF group than in the UF and AF groups.					
37195240	3	44	theme	IDC	449:451	arg1	measurement					453:463	long-term IDC measurement	439:463	long-term IDC measurement in patients with systemic inflammation	439:502	Indirect calorimetry (IDC) is assumed to be useful for acute intensive care; however, there are few studies on long-term IDC measurement in patients with systemic inflammation.					
37195240	5	45	theme	144	741:743	arg1	hours					745:749	72 or 144 hours	735:749	hours	745:749	IDC measurement was performed until 72 or 144 hours.					
37195240	3	46	from	measurement	453:463	arg1	patients					468:475	patients	468:475	patients with systemic inflammation	468:502	Indirect calorimetry (IDC) is assumed to be useful for acute intensive care; however, there are few studies on long-term IDC measurement in patients with systemic inflammation.					
37195240	0	47	theme	LPS-induced	41:51	arg1	model					79:83	an LPS-induced systemic inflammation rat model	38:83	an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments	38:143	Dynamic metabolic changes observed in an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments.					
37195240	2	48	theme	Measuring	218:226	arg1	metabolism					228:237	Measuring metabolism	218:237	Measuring metabolism	218:237	Measuring metabolism is believed to be necessary for the acute sepsis phase to accurately estimate nutrition.					
37195240	3	49	from	studies	428:434	arg1	measurement					453:463	long-term IDC measurement	439:463	long-term IDC measurement in patients with systemic inflammation	439:502	Indirect calorimetry (IDC) is assumed to be useful for acute intensive care; however, there are few studies on long-term IDC measurement in patients with systemic inflammation.					
37195240	6	50	theme	144	849:851	arg1	hours					853:857	72 or 144 hours	843:857	hours	853:857	Body composition was measured at -24 and 72 or 144 hours and tissue weight was measured at 72 or 144 hours.					
37195240	6	51	theme	72	843:844	arg1	hours					853:857	72 or 144 hours	843:857	hours	853:857	Body composition was measured at -24 and 72 or 144 hours and tissue weight was measured at 72 or 144 hours.					
37195240	4	52	theme	control	581:587	arg1	groups					589:594	the lipopolysaccharide (LPS) received or control groups	540:594	the lipopolysaccharide (LPS) received or control groups	540:594	METHODS Rats were categorized into the lipopolysaccharide (LPS) received or control groups; LPS rats were categorized into underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups.					
37195240	11	53	theme	diurnal	1347:1353	arg1	variation					1355:1363	diurnal variation	1347:1363	diurnal variation	1347:1363	In the third phase, diurnal variation recovered in all groups.					
37195240	6	54	theme	Body	752:755	arg1	composition					757:767	Body composition	752:767	Body composition	752:767	Body composition was measured at -24 and 72 or 144 hours and tissue weight was measured at 72 or 144 hours.					
37195240	10	55	theme	higher	1246:1251	arg1	consumption					1260:1270	higher energy consumption	1246:1270	higher energy consumption	1246:1270	In the second and third phases, higher energy consumption occurred in the OF group than in the UF and AF groups.					
37195240	12	56	theme	body	1412:1415	arg1	loss					1424:1427	body weight loss	1412:1427	body weight loss	1412:1427	Muscle atrophy caused body weight loss but fat tissue loss did not occur.					
37195240	4	57	theme	feeding	656:662	arg1	groups					691:696	underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups	628:696	underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups	628:696	METHODS Rats were categorized into the lipopolysaccharide (LPS) received or control groups; LPS rats were categorized into underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups.					
37195240	7	58	theme	LPS	978:980	arg1	group					982:986	the LPS group	974:986	the LPS group compared with the control group until 72 hours	974:1033	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	8	59	theme	UF	1125:1126	arg1	groups					1135:1140	the UF and AF groups	1121:1140	the UF and AF groups	1121:1140	The REE in the OF group was higher than that in the UF and AF groups.					
37195240	14	60	theme	inflammation	1685:1696	arg1	model					1702:1706	the LPS-induced systemic inflammation rat model	1660:1706	the LPS-induced systemic inflammation rat model	1660:1706	This is the first report of long-term IDC measurement using the LPS-induced systemic inflammation rat model.					
37195240	8	61	theme	AF	1132:1133	arg1	groups					1135:1140	the UF and AF groups	1121:1140	the UF and AF groups	1121:1140	The REE in the OF group was higher than that in the UF and AF groups.					
37195240	13	62	theme	systemic	1532:1539	arg1	phase					1554:1558	the acute systemic inflammation phase	1522:1558	the acute systemic inflammation phase owing to differences in calorie intake	1522:1597	CONCLUSIONS We observed metabolic changes with IDC during the acute systemic inflammation phase owing to differences in calorie intake.					
37195240	14	63	theme	LPS-induced	1664:1674	arg1	model					1702:1706	the LPS-induced systemic inflammation rat model	1660:1706	the LPS-induced systemic inflammation rat model	1660:1706	This is the first report of long-term IDC measurement using the LPS-induced systemic inflammation rat model.					
37195240	4	64	theme	overfeeding	674:684	arg1	groups					691:696	underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups	628:696	underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups	628:696	METHODS Rats were categorized into the lipopolysaccharide (LPS) received or control groups; LPS rats were categorized into underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups.					
37195240	7	65	theme	energy	932:937	arg1	REE					952:954	REE	952:954	REE	952:954	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	7	65	theme	energy	932:937	arg1	expenditure					939:949	resting energy expenditure	924:949	resting energy expenditure (REE)	924:955	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	1	66	theme	BACKGROUND	146:155	arg1	management					169:178	BACKGROUND Nutritional management	146:178	BACKGROUND Nutritional management	146:178	BACKGROUND Nutritional management is crucial for severely ill patients.					
37195240	3	67	theme	Indirect	328:335	arg1	IDC					350:352	IDC	350:352	IDC	350:352	Indirect calorimetry (IDC) is assumed to be useful for acute intensive care; however, there are few studies on long-term IDC measurement in patients with systemic inflammation.					
37195240	3	67	theme	Indirect	328:335	arg1	calorimetry					337:347	Indirect calorimetry	328:347	Indirect calorimetry (IDC)	328:353	Indirect calorimetry (IDC) is assumed to be useful for acute intensive care; however, there are few studies on long-term IDC measurement in patients with systemic inflammation.					
37195240	0	68	theme	rat	75:77	arg1	model					79:83	an LPS-induced systemic inflammation rat model	38:83	an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments	38:143	Dynamic metabolic changes observed in an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments.					
37195240	2	69	theme	acute	275:279	arg1	phase					288:292	the acute sepsis phase	271:292	the acute sepsis phase	271:292	Measuring metabolism is believed to be necessary for the acute sepsis phase to accurately estimate nutrition.					
37195240	14	70	theme	IDC	1638:1640	arg1	measurement					1642:1652	long-term IDC measurement	1628:1652	long-term IDC measurement using the LPS-induced systemic inflammation rat model	1628:1706	This is the first report of long-term IDC measurement using the LPS-induced systemic inflammation rat model.					
37195240	7	71	theme	diurnal	903:909	arg1	variation					911:919	diurnal variation	903:919	diurnal variation of resting energy expenditure (REE)	903:955	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	7	72	dep	RESULTS	860:866	arg1	observed					962:969	observed	962:969	observed in the LPS group compared with the control group until 72 hours	962:1033	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	0	73	theme	long-term	102:110	arg1	experiments					133:143	continuous long-term indirect calorimetry experiments	91:143	continuous long-term indirect calorimetry experiments	91:143	Dynamic metabolic changes observed in an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments.					
37195240	9	74	theme	first	1150:1154	arg1	phase					1156:1160	the first phase	1146:1160	the first phase	1146:1160	In the first phase, low energy consumption was observed in all groups.					
37195240	7	75	located	observed	962:969	arg2	loss					895:898	loss	895:898	loss of diurnal variation of resting energy expenditure (REE)	895:955	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	7	75	located	observed	962:969	arg2	consumption					879:889	Low energy consumption	868:889	Low energy consumption	868:889	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	7	75	located	observed	962:969	arg1	group					982:986	the LPS group	974:986	the LPS group compared with the control group until 72 hours	974:1033	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	0	76	theme	calorimetry	121:131	arg1	experiments					133:143	continuous long-term indirect calorimetry experiments	91:143	continuous long-term indirect calorimetry experiments	91:143	Dynamic metabolic changes observed in an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments.					
37195240	4	77	dep	received	569:576	arg1	lipopolysaccharide					544:561	lipopolysaccharide	544:561	lipopolysaccharide (LPS)	544:567	METHODS Rats were categorized into the lipopolysaccharide (LPS) received or control groups; LPS rats were categorized into underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups.					
37195240	4	77	dep	received	569:576	arg1	LPS					564:566	LPS	564:566	LPS	564:566	METHODS Rats were categorized into the lipopolysaccharide (LPS) received or control groups; LPS rats were categorized into underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups.					
37195240	4	78	theme	underfeeding	628:639	arg1	groups					691:696	underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups	628:696	underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups	628:696	METHODS Rats were categorized into the lipopolysaccharide (LPS) received or control groups; LPS rats were categorized into underfeeding (UF), adjusted feeding (AF), and overfeeding (OF) groups.					
37195240	5	79	theme	IDC	699:701	arg1	measurement					703:713	IDC measurement	699:713	IDC measurement	699:713	IDC measurement was performed until 72 or 144 hours.					
37195240	0	80	theme	metabolic	8:16	arg1	changes					18:24	Dynamic metabolic changes	0:24	Dynamic metabolic changes	0:24	Dynamic metabolic changes observed in an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments.					
37195240	9	81	theme	energy	1167:1172	arg1	consumption					1174:1184	low energy consumption	1163:1184	low energy consumption	1163:1184	In the first phase, low energy consumption was observed in all groups.					
37195240	7	82	theme	Low	868:870	arg1	consumption					879:889	Low energy consumption	868:889	Low energy consumption	868:889	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	13	83	from	differences	1569:1579	arg1	intake					1592:1597	calorie intake	1584:1597	calorie intake	1584:1597	CONCLUSIONS We observed metabolic changes with IDC during the acute systemic inflammation phase owing to differences in calorie intake.					
37195240	13	84	theme	metabolic	1488:1496	arg1	changes					1498:1504	metabolic changes	1488:1504	metabolic changes with IDC	1488:1513	CONCLUSIONS We observed metabolic changes with IDC during the acute systemic inflammation phase owing to differences in calorie intake.					
37195240	7	85	theme	LPS	1052:1054	arg1	group					1056:1060	the LPS group	1048:1060	the LPS group	1048:1060	RESULTS Low energy consumption and loss of diurnal variation of resting energy expenditure (REE) were observed in the LPS group compared with the control group until 72 hours, after which the LPS group recovered.					
37195240	3	86	theme	intensive	389:397	arg1	care					399:402	acute intensive care	383:402	acute intensive care	383:402	Indirect calorimetry (IDC) is assumed to be useful for acute intensive care; however, there are few studies on long-term IDC measurement in patients with systemic inflammation.					
37195240	12	87	theme	tissue	1437:1442	arg1	loss					1444:1447	fat tissue loss	1433:1447	fat tissue loss	1433:1447	Muscle atrophy caused body weight loss but fat tissue loss did not occur.					
37195240	0	88	theme	systemic	53:60	arg1	model					79:83	an LPS-induced systemic inflammation rat model	38:83	an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments	38:143	Dynamic metabolic changes observed in an LPS-induced systemic inflammation rat model using continuous long-term indirect calorimetry experiments.					
37195240	3	89	theme	systemic	482:489	arg1	inflammation					491:502	systemic inflammation	482:502	systemic inflammation	482:502	Indirect calorimetry (IDC) is assumed to be useful for acute intensive care; however, there are few studies on long-term IDC measurement in patients with systemic inflammation.					
37195240	3	90	theme	long-term	439:447	arg1	measurement					453:463	long-term IDC measurement	439:463	long-term IDC measurement in patients with systemic inflammation	439:502	Indirect calorimetry (IDC) is assumed to be useful for acute intensive care; however, there are few studies on long-term IDC measurement in patients with systemic inflammation.					
36727418	2	0	from	roll	377:380	arg1	pathogenicity					385:397	pathogenicity	385:397	pathogenicity	385:397	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	3	1	theme	infection	517:525	arg1	impact					502:507	the impact	498:507	the impact of GRBV infection on cell wall metabolism	498:549	Understanding the impact of GRBV infection on cell wall metabolism is important for the development of potential mitigations strategies.					
36727418	6	2	theme	Log	889:891	arg1	changes					898:904	Log fold changes	889:904	Log fold changes	889:904	RESULTS Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell wall solubilization and degradation.					
36727418	11	3	theme	grape	1755:1759	arg1	walls					1766:1770	the grape cell walls	1751:1770	the grape cell walls	1751:1770	These results suggest GRBV alters the grape cell walls, consequently decreasing phenolic extraction during winemaking.					
36727418	7	4	theme	cell	1102:1105	arg1	composition					1112:1122	cell wall composition	1102:1122	cell wall composition	1102:1122	However, these observations did not translate to changes in cell wall composition at either harvest point in diseased grapes potentially suggesting post-transcriptional regulation.					
36727418	0	5	theme	Grapevine	0:8	arg1	virus					21:25	Grapevine red blotch virus	0:25	Grapevine red blotch virus	0:25	Grapevine red blotch virus alters grape skin cell wall composition impacting phenolic extractability during winemaking.					
36727418	9	6	theme	phenolic	1518:1525	arg1	compounds					1527:1535	phenolic compounds	1518:1535	phenolic compounds	1518:1535	Both pectin and pathogenesis-related proteins are known to retain phenolic compounds during winemaking.					
36727418	2	7	with	roll	377:380	arg1	plants					429:434	host plants	424:434	host plants	424:434	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	4	8	theme	transcriptome	652:664	arg1	sequencing					666:675	high-throughput transcriptome sequencing	636:675	high-throughput transcriptome sequencing	636:675	In this study, high-throughput transcriptome sequencing was conducted on Vitis vinifera L. Merlot grapes during ripening.					
36727418	10	9	theme	GRBV	1696:1699	arg1	fruit					1710:1714	GRBV infected fruit	1696:1714	GRBV infected fruit	1696:1714	CONCLUSION Our study corroborates this finding when the percent extractability of flavonols in wines was significantly lower when made from GRBV infected fruit.					
36727418	8	10	theme	pathogenesis-related	1321:1340	arg1	proteins					1342:1349	pathogenesis-related proteins	1321:1349	pathogenesis-related proteins	1321:1349	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	3	11	theme	cell	530:533	arg1	metabolism					540:549	cell wall metabolism	530:549	cell wall metabolism	530:549	Understanding the impact of GRBV infection on cell wall metabolism is important for the development of potential mitigations strategies.					
36727418	5	12	theme	different	823:831	arg1	points					852:857	two different commercial harvest points	819:857	two different commercial harvest points	819:857	The cell wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined.					
36727418	8	13	theme	pectin	1245:1250	arg1	transcripts					1277:1287	pectin methylesterase inhibitor transcripts	1245:1287	pectin methylesterase inhibitor transcripts	1245:1287	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	1	14	theme	blotch	202:207	arg1	disease					209:215	grapevine red blotch disease	188:215	grapevine red blotch disease	188:215	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	8	15	theme	inhibitor	1267:1275	arg1	transcripts					1277:1287	pectin methylesterase inhibitor transcripts	1245:1287	pectin methylesterase inhibitor transcripts	1245:1287	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	6	16	theme	diseased	938:945	arg1	grapes					947:952	diseased grapes	938:952	diseased grapes	938:952	RESULTS Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell wall solubilization and degradation.					
36727418	2	17	from	extractability	450:463	arg1	pathogenicity					385:397	pathogenicity	385:397	pathogenicity	385:397	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	4	18	theme	vinifera	700:707	arg1	grapes					719:724	Vitis vinifera L. Merlot grapes	694:724	Vitis vinifera L. Merlot grapes	694:724	In this study, high-throughput transcriptome sequencing was conducted on Vitis vinifera L. Merlot grapes during ripening.					
36727418	2	19	with	interactions	406:417	arg1	plants					429:434	host plants	424:434	host plants	424:434	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	1	20	theme	Grapevine	131:139	arg1	agent					179:183	the causal agent	168:183	the causal agent of grapevine red blotch disease	168:215	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	1	20	theme	Grapevine	131:139	arg1	GRBV					159:162	GRBV	159:162	GRBV	159:162	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	1	20	theme	Grapevine	131:139	arg1	virus					152:156	BACKGROUND Grapevine red blotch virus	120:156	BACKGROUND Grapevine red blotch virus (GRBV)	120:163	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	4	21	theme	Merlot	712:717	arg1	grapes					719:724	Vitis vinifera L. Merlot grapes	694:724	Vitis vinifera L. Merlot grapes	694:724	In this study, high-throughput transcriptome sequencing was conducted on Vitis vinifera L. Merlot grapes during ripening.					
36727418	3	22	theme	mitigations	597:607	arg1	strategies					609:618	potential mitigations strategies	587:618	potential mitigations strategies	587:618	Understanding the impact of GRBV infection on cell wall metabolism is important for the development of potential mitigations strategies.					
36727418	1	23	theme	blotch	145:150	arg1	agent					179:183	the causal agent	168:183	the causal agent of grapevine red blotch disease	168:215	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	1	23	theme	blotch	145:150	arg1	GRBV					159:162	GRBV	159:162	GRBV	159:162	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	1	23	theme	blotch	145:150	arg1	virus					152:156	BACKGROUND Grapevine red blotch virus	120:156	BACKGROUND Grapevine red blotch virus (GRBV)	120:163	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	8	24	theme	soluble	1390:1396	arg1	proteins					1398:1405	soluble proteins	1390:1405	soluble proteins	1390:1405	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	0	25	theme	wall	50:53	arg1	composition					55:65	grape skin cell wall composition	34:65	grape skin cell wall composition impacting phenolic extractability	34:99	Grapevine red blotch virus alters grape skin cell wall composition impacting phenolic extractability during winemaking.					
36727418	3	26	theme	GRBV	512:515	arg1	infection					517:525	GRBV infection	512:525	GRBV infection	512:525	Understanding the impact of GRBV infection on cell wall metabolism is important for the development of potential mitigations strategies.					
36727418	10	27	dep	CONCLUSION	1556:1565	arg1	corroborates					1577:1588	corroborates	1577:1588	corroborates this finding when the percent extractability of flavonols in wines was significantly lower when made from GRBV infected fruit	1577:1714	CONCLUSION Our study corroborates this finding when the percent extractability of flavonols in wines was significantly lower when made from GRBV infected fruit.					
36727418	5	28	from	points	852:857	arg1	content					779:785	phenolic content	770:785	phenolic content	770:785	The cell wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined.					
36727418	5	28	from	points	852:857	arg1	extractability					801:814	phenolic extractability	792:814	phenolic extractability	792:814	The cell wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined.					
36727418	5	28	from	points	852:857	arg1	composition					757:767	The cell wall composition	743:767	The cell wall composition	743:767	The cell wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined.					
36727418	2	29	theme	phenolic	441:448	arg1	extractability					450:463	phenolic extractability	441:463	phenolic extractability	441:463	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	7	30	from	point	1142:1146	arg1	grapes					1160:1165	diseased grapes	1151:1165	diseased grapes	1151:1165	However, these observations did not translate to changes in cell wall composition at either harvest point in diseased grapes potentially suggesting post-transcriptional regulation.					
36727418	8	31	from	increases	1366:1374	arg1	pectin					1379:1384	pectin	1379:1384	pectin	1379:1384	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	8	31	from	increases	1366:1374	arg1	proteins					1398:1405	soluble proteins	1390:1405	soluble proteins	1390:1405	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	8	31	from	increases	1366:1374	arg1	walls					1415:1419	cell walls	1410:1419	cell walls of diseased grapes at harvest	1410:1449	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	6	32	theme	transcripts	976:986	arg1	harvest					957:963	harvest	957:963	harvest of several transcripts involved in cell wall solubilization and degradation	957:1039	RESULTS Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell wall solubilization and degradation.					
36727418	6	33	dep	wall	1005:1008	arg1	solubilization					1010:1023	solubilization	1010:1023	solubilization	1010:1023	RESULTS Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell wall solubilization and degradation.					
36727418	8	34	theme	diseased	1424:1431	arg1	grapes					1433:1438	diseased grapes	1424:1438	diseased grapes at harvest	1424:1449	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	2	35	theme	cell	353:356	arg1	wall					358:361	the cell wall	349:361	the cell wall	349:361	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	7	36	theme	post-transcriptional	1190:1209	arg1	regulation					1211:1220	post-transcriptional regulation	1190:1220	post-transcriptional regulation	1190:1220	However, these observations did not translate to changes in cell wall composition at either harvest point in diseased grapes potentially suggesting post-transcriptional regulation.					
36727418	11	37	dep	suggest	1731:1737	arg1	alters					1744:1749	alters	1744:1749	alters	1744:1749	These results suggest GRBV alters the grape cell walls, consequently decreasing phenolic extraction during winemaking.					
36727418	7	38	from	changes	1091:1097	arg1	composition					1112:1122	cell wall composition	1102:1122	cell wall composition	1102:1122	However, these observations did not translate to changes in cell wall composition at either harvest point in diseased grapes potentially suggesting post-transcriptional regulation.					
36727418	0	39	theme	blotch	14:19	arg1	virus					21:25	Grapevine red blotch virus	0:25	Grapevine red blotch virus	0:25	Grapevine red blotch virus alters grape skin cell wall composition impacting phenolic extractability during winemaking.					
36727418	2	40	theme	large	371:375	arg1	roll					377:380	a large roll	369:380	a large roll in pathogenicity	369:397	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	5	41	theme	phenolic	770:777	arg1	content					779:785	phenolic content	770:785	phenolic content	770:785	The cell wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined.					
36727418	7	42	theme	diseased	1151:1158	arg1	grapes					1160:1165	diseased grapes	1151:1165	diseased grapes	1151:1165	However, these observations did not translate to changes in cell wall composition at either harvest point in diseased grapes potentially suggesting post-transcriptional regulation.					
36727418	2	43	theme	host	424:427	arg1	plants					429:434	host plants	424:434	host plants	424:434	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	5	44	theme	cell	747:750	arg1	composition					757:767	The cell wall composition	743:767	The cell wall composition	743:767	The cell wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined.					
36727418	0	45	theme	skin	40:43	arg1	composition					55:65	grape skin cell wall composition	34:65	grape skin cell wall composition impacting phenolic extractability	34:99	Grapevine red blotch virus alters grape skin cell wall composition impacting phenolic extractability during winemaking.					
36727418	1	46	theme	disease	209:215	arg1	agent					179:183	the causal agent	168:183	the causal agent of grapevine red blotch disease	168:215	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	1	46	theme	disease	209:215	arg1	virus					152:156	BACKGROUND Grapevine red blotch virus	120:156	BACKGROUND Grapevine red blotch virus (GRBV)	120:163	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	0	47	theme	cell	45:48	arg1	composition					55:65	grape skin cell wall composition	34:65	grape skin cell wall composition impacting phenolic extractability	34:99	Grapevine red blotch virus alters grape skin cell wall composition impacting phenolic extractability during winemaking.					
36727418	6	48	theme	strong	918:923	arg1	induction					925:933	a strong induction	916:933	a strong induction in diseased grapes	916:952	RESULTS Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell wall solubilization and degradation.					
36727418	10	49	theme	flavonols	1638:1646	arg1	lower					1675:1679	lower	1675:1679	lower	1675:1679	CONCLUSION Our study corroborates this finding when the percent extractability of flavonols in wines was significantly lower when made from GRBV infected fruit.					
36727418	10	49	theme	flavonols	1638:1646	arg1	extractability					1620:1633	the percent extractability	1608:1633	the percent extractability of flavonols in wines	1608:1655	CONCLUSION Our study corroborates this finding when the percent extractability of flavonols in wines was significantly lower when made from GRBV infected fruit.					
36727418	5	50	theme	wall	752:755	arg1	composition					757:767	The cell wall composition	743:767	The cell wall composition	743:767	The cell wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined.					
36727418	8	51	from	harvest	1443:1449	arg1	walls					1415:1419	cell walls	1410:1419	cell walls of diseased grapes at harvest	1410:1449	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	8	51	from	harvest	1443:1449	arg1	grapes					1433:1438	diseased grapes	1424:1438	diseased grapes at harvest	1424:1449	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	1	52	theme	grapevine	188:196	arg1	disease					209:215	grapevine red blotch disease	188:215	grapevine red blotch disease	188:215	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	7	53	theme	wall	1107:1110	arg1	composition					1112:1122	cell wall composition	1102:1122	cell wall composition	1102:1122	However, these observations did not translate to changes in cell wall composition at either harvest point in diseased grapes potentially suggesting post-transcriptional regulation.					
36727418	2	54	theme	grape	264:268	arg1	modifications					280:292	grape cell wall modifications	264:292	grape cell wall modifications during GRBV infection	264:314	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	1	55	theme	grape	239:243	arg1	ripening					245:252	grape ripening	239:252	grape ripening	239:252	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	6	56	theme	fold	893:896	arg1	changes					898:904	Log fold changes	889:904	Log fold changes	889:904	RESULTS Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell wall solubilization and degradation.					
36727418	8	57	theme	transcripts	1277:1287	arg1	induction					1232:1240	Moderate induction	1223:1240	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins	1223:1349	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	10	58	theme	infected	1701:1708	arg1	fruit					1710:1714	GRBV infected fruit	1696:1714	GRBV infected fruit	1696:1714	CONCLUSION Our study corroborates this finding when the percent extractability of flavonols in wines was significantly lower when made from GRBV infected fruit.					
36727418	11	59	theme	cell	1761:1764	arg1	walls					1766:1770	the grape cell walls	1751:1770	the grape cell walls	1751:1770	These results suggest GRBV alters the grape cell walls, consequently decreasing phenolic extraction during winemaking.					
36727418	3	60	theme	wall	535:538	arg1	metabolism					540:549	cell wall metabolism	530:549	cell wall metabolism	530:549	Understanding the impact of GRBV infection on cell wall metabolism is important for the development of potential mitigations strategies.					
36727418	10	61	theme	percent	1612:1618	arg1	lower					1675:1679	lower	1675:1679	lower	1675:1679	CONCLUSION Our study corroborates this finding when the percent extractability of flavonols in wines was significantly lower when made from GRBV infected fruit.					
36727418	10	61	theme	percent	1612:1618	arg1	extractability					1620:1633	the percent extractability	1608:1633	the percent extractability of flavonols in wines	1608:1655	CONCLUSION Our study corroborates this finding when the percent extractability of flavonols in wines was significantly lower when made from GRBV infected fruit.					
36727418	1	62	theme	causal	172:177	arg1	agent					179:183	the causal agent	168:183	the causal agent of grapevine red blotch disease	168:215	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	1	62	theme	causal	172:177	arg1	virus					152:156	BACKGROUND Grapevine red blotch virus	120:156	BACKGROUND Grapevine red blotch virus (GRBV)	120:163	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	6	63	theme	several	968:974	arg1	transcripts					976:986	several transcripts	968:986	several transcripts involved in cell wall solubilization and degradation	968:1039	RESULTS Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell wall solubilization and degradation.					
36727418	4	64	theme	high-throughput	636:650	arg1	sequencing					666:675	high-throughput transcriptome sequencing	636:675	high-throughput transcriptome sequencing	636:675	In this study, high-throughput transcriptome sequencing was conducted on Vitis vinifera L. Merlot grapes during ripening.					
36727418	2	65	theme	wall	275:278	arg1	modifications					280:292	grape cell wall modifications	264:292	grape cell wall modifications during GRBV infection	264:314	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	8	66	theme	Moderate	1223:1230	arg1	induction					1232:1240	Moderate induction	1223:1240	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins	1223:1349	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	8	67	theme	transcripts	1293:1303	arg1	induction					1232:1240	Moderate induction	1223:1240	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins	1223:1349	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	5	68	theme	commercial	833:842	arg1	points					852:857	two different commercial harvest points	819:857	two different commercial harvest points	819:857	The cell wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined.					
36727418	1	69	theme	BACKGROUND	120:129	arg1	agent					179:183	the causal agent	168:183	the causal agent of grapevine red blotch disease	168:215	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	1	69	theme	BACKGROUND	120:129	arg1	GRBV					159:162	GRBV	159:162	GRBV	159:162	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	1	69	theme	BACKGROUND	120:129	arg1	virus					152:156	BACKGROUND Grapevine red blotch virus	120:156	BACKGROUND Grapevine red blotch virus (GRBV)	120:163	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	8	70	theme	methylesterase	1252:1265	arg1	transcripts					1277:1287	pectin methylesterase inhibitor transcripts	1245:1287	pectin methylesterase inhibitor transcripts	1245:1287	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	4	71	theme	Vitis	694:698	arg1	vinifera					700:707	Vitis vinifera	694:707	Vitis vinifera L. Merlot grapes	694:724	In this study, high-throughput transcriptome sequencing was conducted on Vitis vinifera L. Merlot grapes during ripening.					
36727418	3	72	theme	potential	587:595	arg1	strategies					609:618	potential mitigations strategies	587:618	potential mitigations strategies	587:618	Understanding the impact of GRBV infection on cell wall metabolism is important for the development of potential mitigations strategies.					
36727418	1	73	theme	red	141:143	arg1	agent					179:183	the causal agent	168:183	the causal agent of grapevine red blotch disease	168:215	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	1	73	theme	red	141:143	arg1	GRBV					159:162	GRBV	159:162	GRBV	159:162	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	1	73	theme	red	141:143	arg1	virus					152:156	BACKGROUND Grapevine red blotch virus	120:156	BACKGROUND Grapevine red blotch virus (GRBV)	120:163	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	4	74	theme	L.	709:710	arg1	grapes					719:724	Vitis vinifera L. Merlot grapes	694:724	Vitis vinifera L. Merlot grapes	694:724	In this study, high-throughput transcriptome sequencing was conducted on Vitis vinifera L. Merlot grapes during ripening.					
36727418	3	75	from	impact	502:507	arg1	metabolism					540:549	cell wall metabolism	530:549	cell wall metabolism	530:549	Understanding the impact of GRBV infection on cell wall metabolism is important for the development of potential mitigations strategies.					
36727418	0	76	theme	phenolic	77:84	arg1	extractability					86:99	phenolic extractability	77:99	phenolic extractability	77:99	Grapevine red blotch virus alters grape skin cell wall composition impacting phenolic extractability during winemaking.					
36727418	6	77	theme	cell	1000:1003	arg1	wall					1005:1008	cell wall solubilization and degradation	1000:1039	wall	1005:1008	RESULTS Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell wall solubilization and degradation.					
36727418	2	78	from	interactions	406:417	arg1	pathogenicity					385:397	pathogenicity	385:397	pathogenicity	385:397	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	2	79	theme	cell	270:273	arg1	modifications					280:292	grape cell wall modifications	264:292	grape cell wall modifications during GRBV infection	264:314	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	10	80	from	extractability	1620:1633	arg1	wines					1651:1655	wines	1651:1655	wines	1651:1655	CONCLUSION Our study corroborates this finding when the percent extractability of flavonols in wines was significantly lower when made from GRBV infected fruit.					
36727418	8	81	theme	cell	1410:1413	arg1	walls					1415:1419	cell walls	1410:1419	cell walls of diseased grapes at harvest	1410:1449	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	2	82	with	extractability	450:463	arg1	plants					429:434	host plants	424:434	host plants	424:434	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	0	83	theme	red	10:12	arg1	virus					21:25	Grapevine red blotch virus	0:25	Grapevine red blotch virus	0:25	Grapevine red blotch virus alters grape skin cell wall composition impacting phenolic extractability during winemaking.					
36727418	2	84	theme	viral	400:404	arg1	interactions					406:417	viral interactions	400:417	viral interactions with host plants	400:434	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	8	85	theme	grapes	1433:1438	arg1	walls					1415:1419	cell walls	1410:1419	cell walls of diseased grapes at harvest	1410:1449	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	5	86	theme	phenolic	792:799	arg1	extractability					801:814	phenolic extractability	792:814	phenolic extractability	792:814	The cell wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined.					
36727418	11	87	theme	phenolic	1797:1804	arg1	extraction					1806:1815	phenolic extraction	1797:1815	phenolic extraction	1797:1815	These results suggest GRBV alters the grape cell walls, consequently decreasing phenolic extraction during winemaking.					
36727418	0	88	theme	grape	34:38	arg1	composition					55:65	grape skin cell wall composition	34:65	grape skin cell wall composition impacting phenolic extractability	34:99	Grapevine red blotch virus alters grape skin cell wall composition impacting phenolic extractability during winemaking.					
36727418	1	89	theme	red	198:200	arg1	disease					209:215	grapevine red blotch disease	188:215	grapevine red blotch disease	188:215	BACKGROUND Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening.					
36727418	3	90	theme	strategies	609:618	arg1	development					572:582	the development	568:582	the development of potential mitigations strategies	568:618	Understanding the impact of GRBV infection on cell wall metabolism is important for the development of potential mitigations strategies.					
36727418	7	91	theme	harvest	1134:1140	arg1	point					1142:1146	either harvest point	1127:1146	either harvest point in diseased grapes	1127:1165	However, these observations did not translate to changes in cell wall composition at either harvest point in diseased grapes potentially suggesting post-transcriptional regulation.					
36727418	6	92	from	induction	925:933	arg1	grapes					947:952	diseased grapes	938:952	diseased grapes	938:952	RESULTS Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell wall solubilization and degradation.					
36727418	6	93	dep	RESULTS	881:887	arg1	indicated					906:914	indicated	906:914	indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell wall solubilization and degradation	906:1039	RESULTS Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell wall solubilization and degradation.					
36727418	2	94	theme	GRBV	301:304	arg1	infection					306:314	GRBV infection	301:314	GRBV infection	301:314	However, grape cell wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large roll in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking.					
36727418	8	95	from	walls	1415:1419	arg1	harvest					1443:1449	harvest	1443:1449	harvest	1443:1449	Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest.					
36727418	5	96	theme	harvest	844:850	arg1	points					852:857	two different commercial harvest points	819:857	two different commercial harvest points	819:857	The cell wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined.					
36727418	9	97	theme	pathogenesis-related	1468:1487	arg1	proteins					1489:1496	pathogenesis-related proteins	1468:1496	pathogenesis-related proteins	1468:1496	Both pectin and pathogenesis-related proteins are known to retain phenolic compounds during winemaking.					
36990416	4	0	theme	color	856:860	arg1	change					862:867	the total color change	846:867	the total color change (ΔE)	846:872	The whiteness index (WI) of the films decreased from 63.4 to 47.79, while the total color change (ΔE) increased significantly (p < 0.05) with the increase in GSO-NE concentration.					
36990416	4	0	theme	color	856:860	arg1	ΔE					870:871	ΔE	870:871	ΔE	870:871	The whiteness index (WI) of the films decreased from 63.4 to 47.79, while the total color change (ΔE) increased significantly (p < 0.05) with the increase in GSO-NE concentration.					
36990416	3	1	theme	GSO-NE	658:663	arg1	incorporation					641:653	incorporation	641:653	incorporation of GSO-NE at 6 % concentration	641:684	The results revealed that incorporation of GSO-NE at 6 % concentration decreased the tensile strength (TS) and puncture force (PF) significantly (p < 0.05).					
36990416	3	2	from	concentration	672:684	arg1	incorporation					641:653	incorporation	641:653	incorporation of GSO-NE at 6 % concentration	641:684	The results revealed that incorporation of GSO-NE at 6 % concentration decreased the tensile strength (TS) and puncture force (PF) significantly (p < 0.05).					
36990416	6	3	theme	GSO-NE	1118:1123	arg1	incorporation					1101:1113	The incorporation	1097:1113	The incorporation of GSO-NE into the films	1097:1138	The incorporation of GSO-NE into the films led to the formation of a slightly porous structure.					
36990416	2	4	with	films	556:560	arg1	properties					603:612	improved physical and antibacterial properties	567:612	improved physical and antibacterial properties	567:612	In this study, ultrasonic treatment was used to prepare GSO-NE, and different levels (2, 4, and 6 %) of nanoemulsioned GSO were incorporated into gelatin (Ge)/sodium alginate (SA)-based films to produce films with improved physical and antibacterial properties.					
36990416	7	5	theme	content	1304:1310	arg1	%					1317:1317	moisture content (MC) %, and water solubility (WS) %	1295:1346	%	1317:1317	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	7	5	theme	content	1304:1310	arg1	permeability					1275:1286	the water vapor permeability	1259:1286	the water vapor permeability (WVP)	1259:1292	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	9	6	theme	Ge/SA/GSO-NE	1454:1465	arg1	films					1467:1471	Ge/SA/GSO-NE films	1454:1471	Ge/SA/GSO-NE films	1454:1471	Ge/SA/GSO-NE films were found to be effective against both Gram-positive and Gram-negative bacteria.					
36990416	10	7	contain	containing	1581:1590	arg1	films					1575:1579	The prepared active films	1555:1579	The prepared active films containing GSO-NE	1555:1597	The prepared active films containing GSO-NE had a high potential for preventing food spoilage in food packaging.					
36990416	10	7	contain	containing	1581:1590	arg2	GSO-NE					1592:1597	GSO-NE	1592:1597	GSO-NE	1592:1597	The prepared active films containing GSO-NE had a high potential for preventing food spoilage in food packaging.					
36990416	5	8	theme	thermal	1057:1063	arg1	stability					1065:1073	the thermal stability	1053:1073	the thermal stability of Ge/SA-based films	1053:1094	Thermogravimetric analysis (TGA) results showed that GSO-NE at different concentrations had improved the thermal stability of Ge/SA-based films.					
36990416	0	9	theme	alginate	105:112	arg1	blends					119:124	gelatin‑sodium alginate film blends	90:124	gelatin‑sodium alginate film blends	90:124	Effects of grape seed oil nanoemulsion on physicochemical and antibacterial properties of gelatin‑sodium alginate film blends.					
36990416	0	10	from	Effects	0:6	arg1	properties					76:85	physicochemical and antibacterial properties	42:85	physicochemical and antibacterial properties of gelatin‑sodium alginate film blends	42:124	Effects of grape seed oil nanoemulsion on physicochemical and antibacterial properties of gelatin‑sodium alginate film blends.					
36990416	1	11	theme	varying	233:239	arg1	concentrations					241:254	varying concentrations	233:254	varying concentrations	233:254	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	0	12	theme	gelatin‑sodium	90:103	arg1	blends					119:124	gelatin‑sodium alginate film blends	90:124	gelatin‑sodium alginate film blends	90:124	Effects of grape seed oil nanoemulsion on physicochemical and antibacterial properties of gelatin‑sodium alginate film blends.					
36990416	1	13	theme	resulting	336:344	arg1	films					346:350	the resulting films	332:350	the resulting films	332:350	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	3	14	dep	significantly	746:758	arg1	p < 0.05					761:768	p < 0.05	761:768	p < 0.05	761:768	The results revealed that incorporation of GSO-NE at 6 % concentration decreased the tensile strength (TS) and puncture force (PF) significantly (p < 0.05).					
36990416	7	15	theme	vapor	1269:1273	arg1	%					1317:1317	moisture content (MC) %, and water solubility (WS) %	1295:1346	%	1317:1317	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	7	15	theme	vapor	1269:1273	arg1	WVP					1289:1291	WVP	1289:1291	WVP	1289:1291	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	7	15	theme	vapor	1269:1273	arg1	permeability					1275:1286	the water vapor permeability	1259:1286	the water vapor permeability (WVP)	1259:1292	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	7	15	theme	vapor	1269:1273	arg1	%					1346:1346	moisture content (MC) %, and water solubility (WS) %	1295:1346	%	1346:1346	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	4	16	theme	total	850:854	arg1	change					862:867	the total color change	846:867	the total color change (ΔE)	846:872	The whiteness index (WI) of the films decreased from 63.4 to 47.79, while the total color change (ΔE) increased significantly (p < 0.05) with the increase in GSO-NE concentration.					
36990416	4	16	theme	total	850:854	arg1	ΔE					870:871	ΔE	870:871	ΔE	870:871	The whiteness index (WI) of the films decreased from 63.4 to 47.79, while the total color change (ΔE) increased significantly (p < 0.05) with the increase in GSO-NE concentration.					
36990416	3	17	theme	puncture	726:733	arg1	PF					742:743	PF	742:743	PF	742:743	The results revealed that incorporation of GSO-NE at 6 % concentration decreased the tensile strength (TS) and puncture force (PF) significantly (p < 0.05).					
36990416	3	17	theme	puncture	726:733	arg1	force					735:739	puncture force	726:739	puncture force (PF)	726:744	The results revealed that incorporation of GSO-NE at 6 % concentration decreased the tensile strength (TS) and puncture force (PF) significantly (p < 0.05).					
36990416	0	18	theme	blends	119:124	arg1	properties					76:85	physicochemical and antibacterial properties	42:85	physicochemical and antibacterial properties of gelatin‑sodium alginate film blends	42:124	Effects of grape seed oil nanoemulsion on physicochemical and antibacterial properties of gelatin‑sodium alginate film blends.					
36990416	1	19	theme	films	346:350	arg1	properties					318:327	the physicochemical and antimicrobial properties	280:327	the physicochemical and antimicrobial properties of the resulting films	280:350	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	7	20	theme	water	1263:1267	arg1	%					1317:1317	moisture content (MC) %, and water solubility (WS) %	1295:1346	%	1317:1317	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	7	20	theme	water	1263:1267	arg1	WVP					1289:1291	WVP	1289:1291	WVP	1289:1291	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	7	20	theme	water	1263:1267	arg1	permeability					1275:1286	the water vapor permeability	1259:1286	the water vapor permeability (WVP)	1259:1292	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	7	20	theme	water	1263:1267	arg1	%					1346:1346	moisture content (MC) %, and water solubility (WS) %	1295:1346	%	1346:1346	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	2	21	theme	ultrasonic	368:377	arg1	treatment					379:387	ultrasonic treatment	368:387	ultrasonic treatment	368:387	In this study, ultrasonic treatment was used to prepare GSO-NE, and different levels (2, 4, and 6 %) of nanoemulsioned GSO were incorporated into gelatin (Ge)/sodium alginate (SA)-based films to produce films with improved physical and antibacterial properties.					
36990416	0	22	theme	film	114:117	arg1	blends					119:124	gelatin‑sodium alginate film blends	90:124	gelatin‑sodium alginate film blends	90:124	Effects of grape seed oil nanoemulsion on physicochemical and antibacterial properties of gelatin‑sodium alginate film blends.					
36990416	5	23	theme	Thermogravimetric	952:968	arg1	TGA					980:982	TGA	980:982	TGA	980:982	Thermogravimetric analysis (TGA) results showed that GSO-NE at different concentrations had improved the thermal stability of Ge/SA-based films.					
36990416	5	23	theme	Thermogravimetric	952:968	arg1	analysis					970:977	Thermogravimetric analysis	952:977	Thermogravimetric analysis (TGA) results	952:991	Thermogravimetric analysis (TGA) results showed that GSO-NE at different concentrations had improved the thermal stability of Ge/SA-based films.					
36990416	2	24	theme	-based	532:537	arg1	films					539:543	gelatin (Ge)/sodium alginate (SA)-based films	499:543	gelatin (Ge)/sodium alginate (SA)-based films	499:543	In this study, ultrasonic treatment was used to prepare GSO-NE, and different levels (2, 4, and 6 %) of nanoemulsioned GSO were incorporated into gelatin (Ge)/sodium alginate (SA)-based films to produce films with improved physical and antibacterial properties.					
36990416	2	25	theme	antibacterial	589:601	arg1	properties					603:612	improved physical and antibacterial properties	567:612	improved physical and antibacterial properties	567:612	In this study, ultrasonic treatment was used to prepare GSO-NE, and different levels (2, 4, and 6 %) of nanoemulsioned GSO were incorporated into gelatin (Ge)/sodium alginate (SA)-based films to produce films with improved physical and antibacterial properties.					
36990416	5	26	theme	films	1090:1094	arg1	stability					1065:1073	the thermal stability	1053:1073	the thermal stability of Ge/SA-based films	1053:1094	Thermogravimetric analysis (TGA) results showed that GSO-NE at different concentrations had improved the thermal stability of Ge/SA-based films.					
36990416	8	27	theme	composite	1378:1386	arg1	films					1388:1392	All composite films	1374:1392	All composite films	1374:1392	All composite films exhibited hydrophobic surfaces with contact angles θ > 90°.					
36990416	5	28	theme	analysis	970:977	arg1	results					985:991	Thermogravimetric analysis (TGA) results	952:991	Thermogravimetric analysis (TGA) results	952:991	Thermogravimetric analysis (TGA) results showed that GSO-NE at different concentrations had improved the thermal stability of Ge/SA-based films.					
36990416	7	29	theme	MC	1313:1314	arg1	%					1317:1317	moisture content (MC) %, and water solubility (WS) %	1295:1346	%	1317:1317	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	7	29	theme	MC	1313:1314	arg1	permeability					1275:1286	the water vapor permeability	1259:1286	the water vapor permeability (WVP)	1259:1292	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	5	30	theme	different	1015:1023	arg1	concentrations					1025:1038	different concentrations	1015:1038	different concentrations	1015:1038	Thermogravimetric analysis (TGA) results showed that GSO-NE at different concentrations had improved the thermal stability of Ge/SA-based films.					
36990416	10	31	theme	high	1605:1608	arg1	potential					1610:1618	a high potential	1603:1618	a high potential for preventing food spoilage in food packaging	1603:1665	The prepared active films containing GSO-NE had a high potential for preventing food spoilage in food packaging.					
36990416	7	32	theme	moisture	1295:1302	arg1	%					1317:1317	moisture content (MC) %, and water solubility (WS) %	1295:1346	%	1317:1317	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	7	32	theme	moisture	1295:1302	arg1	permeability					1275:1286	the water vapor permeability	1259:1286	the water vapor permeability (WVP)	1259:1292	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	10	33	theme	active	1568:1573	arg1	films					1575:1579	The prepared active films	1555:1579	The prepared active films containing GSO-NE	1555:1597	The prepared active films containing GSO-NE had a high potential for preventing food spoilage in food packaging.					
36990416	4	34	theme	films	804:808	arg1	WI					793:794	WI	793:794	WI	793:794	The whiteness index (WI) of the films decreased from 63.4 to 47.79, while the total color change (ΔE) increased significantly (p < 0.05) with the increase in GSO-NE concentration.					
36990416	4	34	theme	films	804:808	arg1	index					786:790	The whiteness index	772:790	The whiteness index (WI) of the films	772:808	The whiteness index (WI) of the films decreased from 63.4 to 47.79, while the total color change (ΔE) increased significantly (p < 0.05) with the increase in GSO-NE concentration.					
36990416	3	35	theme	6 	668:669	arg1	%					670:670	%	670:670	%	670:670	The results revealed that incorporation of GSO-NE at 6 % concentration decreased the tensile strength (TS) and puncture force (PF) significantly (p < 0.05).					
36990416	5	36	from	concentrations	1025:1038	arg1	GSO-NE					1005:1010	GSO-NE	1005:1010	GSO-NE at different concentrations	1005:1038	Thermogravimetric analysis (TGA) results showed that GSO-NE at different concentrations had improved the thermal stability of Ge/SA-based films.					
36990416	2	37	theme	physical	576:583	arg1	properties					603:612	improved physical and antibacterial properties	567:612	improved physical and antibacterial properties	567:612	In this study, ultrasonic treatment was used to prepare GSO-NE, and different levels (2, 4, and 6 %) of nanoemulsioned GSO were incorporated into gelatin (Ge)/sodium alginate (SA)-based films to produce films with improved physical and antibacterial properties.					
36990416	1	38	theme	grape	191:195	arg1	NE					226:227	NE	226:227	NE	226:227	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	1	38	theme	grape	191:195	arg1	nanoemulsion					212:223	grape seed oil (GSO) nanoemulsion	191:223	grape seed oil (GSO) nanoemulsion (NE)	191:228	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	0	39	theme	seed	17:20	arg1	nanoemulsion					26:37	grape seed oil nanoemulsion	11:37	grape seed oil nanoemulsion	11:37	Effects of grape seed oil nanoemulsion on physicochemical and antibacterial properties of gelatin‑sodium alginate film blends.					
36990416	3	40	theme	%	670:670	arg1	concentration					672:684	6 % concentration	668:684	6 % concentration	668:684	The results revealed that incorporation of GSO-NE at 6 % concentration decreased the tensile strength (TS) and puncture force (PF) significantly (p < 0.05).					
36990416	2	41	theme	improved	567:574	arg1	properties					603:612	improved physical and antibacterial properties	567:612	improved physical and antibacterial properties	567:612	In this study, ultrasonic treatment was used to prepare GSO-NE, and different levels (2, 4, and 6 %) of nanoemulsioned GSO were incorporated into gelatin (Ge)/sodium alginate (SA)-based films to produce films with improved physical and antibacterial properties.					
36990416	7	42	theme	%	1232:1232	arg1	concentrations					1234:1247	4 and 6 % concentrations	1224:1247	4 and 6 % concentrations	1224:1247	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	10	43	theme	food	1635:1638	arg1	spoilage					1640:1647	food spoilage	1635:1647	food spoilage in food packaging	1635:1665	The prepared active films containing GSO-NE had a high potential for preventing food spoilage in food packaging.					
36990416	1	44	theme	seed	197:200	arg1	NE					226:227	NE	226:227	NE	226:227	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	1	44	theme	seed	197:200	arg1	nanoemulsion					212:223	grape seed oil (GSO) nanoemulsion	191:223	grape seed oil (GSO) nanoemulsion (NE)	191:228	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	0	45	theme	grape	11:15	arg1	nanoemulsion					26:37	grape seed oil nanoemulsion	11:37	grape seed oil nanoemulsion	11:37	Effects of grape seed oil nanoemulsion on physicochemical and antibacterial properties of gelatin‑sodium alginate film blends.					
36990416	1	46	theme	film	265:268	arg1	matrix					270:275	the film matrix	261:275	the film matrix	261:275	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	6	47	theme	structure	1182:1190	arg1	formation					1151:1159	the formation	1147:1159	the formation of a slightly porous structure	1147:1190	The incorporation of GSO-NE into the films led to the formation of a slightly porous structure.					
36990416	7	48	theme	WS	1342:1343	arg1	%					1346:1346	moisture content (MC) %, and water solubility (WS) %	1295:1346	%	1346:1346	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	7	48	theme	WS	1342:1343	arg1	permeability					1275:1286	the water vapor permeability	1259:1286	the water vapor permeability (WVP)	1259:1292	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	1	49	theme	oil	202:204	arg1	NE					226:227	NE	226:227	NE	226:227	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	1	49	theme	oil	202:204	arg1	nanoemulsion					212:223	grape seed oil (GSO) nanoemulsion	191:223	grape seed oil (GSO) nanoemulsion (NE)	191:228	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	0	50	theme	nanoemulsion	26:37	arg1	Effects					0:6	Effects	0:6	Effects of grape seed oil nanoemulsion on physicochemical and antibacterial properties of gelatin‑sodium alginate film blends.	0:125	Effects of grape seed oil nanoemulsion on physicochemical and antibacterial properties of gelatin‑sodium alginate film blends.					
36990416	10	51	contain	had	1599:1601	arg1	films					1575:1579	The prepared active films	1555:1579	The prepared active films containing GSO-NE	1555:1597	The prepared active films containing GSO-NE had a high potential for preventing food spoilage in food packaging.					
36990416	10	51	contain	had	1599:1601	arg2	potential					1610:1618	a high potential	1603:1618	a high potential for preventing food spoilage in food packaging	1603:1665	The prepared active films containing GSO-NE had a high potential for preventing food spoilage in food packaging.					
36990416	2	52	theme	GSO	472:474	arg1	levels					431:436	different levels	421:436	different levels (2, 4, and 6 %) of nanoemulsioned GSO	421:474	In this study, ultrasonic treatment was used to prepare GSO-NE, and different levels (2, 4, and 6 %) of nanoemulsioned GSO were incorporated into gelatin (Ge)/sodium alginate (SA)-based films to produce films with improved physical and antibacterial properties.					
36990416	2	52	theme	GSO	472:474	arg1	%					451:451	2, 4, and 6 %	439:451	2, 4, and 6 %	439:451	In this study, ultrasonic treatment was used to prepare GSO-NE, and different levels (2, 4, and 6 %) of nanoemulsioned GSO were incorporated into gelatin (Ge)/sodium alginate (SA)-based films to produce films with improved physical and antibacterial properties.					
36990416	8	53	theme	contact	1430:1436	arg1	angles					1438:1443	contact angles	1430:1443	contact angles θ > 90°	1430:1451	All composite films exhibited hydrophobic surfaces with contact angles θ > 90°.					
36990416	0	54	theme	oil	22:24	arg1	nanoemulsion					26:37	grape seed oil nanoemulsion	11:37	grape seed oil nanoemulsion	11:37	Effects of grape seed oil nanoemulsion on physicochemical and antibacterial properties of gelatin‑sodium alginate film blends.					
36990416	2	55	theme	nanoemulsioned	457:470	arg1	GSO					472:474	nanoemulsioned GSO	457:474	nanoemulsioned GSO	457:474	In this study, ultrasonic treatment was used to prepare GSO-NE, and different levels (2, 4, and 6 %) of nanoemulsioned GSO were incorporated into gelatin (Ge)/sodium alginate (SA)-based films to produce films with improved physical and antibacterial properties.					
36990416	6	56	theme	porous	1175:1180	arg1	structure					1182:1190	a slightly porous structure	1164:1190	a slightly porous structure	1164:1190	The incorporation of GSO-NE into the films led to the formation of a slightly porous structure.					
36990416	7	57	theme	4	1224:1224	arg1	concentrations					1234:1247	4 and 6 % concentrations	1224:1247	4 and 6 % concentrations	1224:1247	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	1	58	theme	GSO	207:209	arg1	NE					226:227	NE	226:227	NE	226:227	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	1	58	theme	GSO	207:209	arg1	nanoemulsion					212:223	grape seed oil (GSO) nanoemulsion	191:223	grape seed oil (GSO) nanoemulsion (NE)	191:228	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	0	59	theme	physicochemical	42:56	arg1	properties					76:85	physicochemical and antibacterial properties	42:85	physicochemical and antibacterial properties of gelatin‑sodium alginate film blends	42:124	Effects of grape seed oil nanoemulsion on physicochemical and antibacterial properties of gelatin‑sodium alginate film blends.					
36990416	3	60	theme	tensile	700:706	arg1	TS					718:719	TS	718:719	TS	718:719	The results revealed that incorporation of GSO-NE at 6 % concentration decreased the tensile strength (TS) and puncture force (PF) significantly (p < 0.05).					
36990416	3	60	theme	tensile	700:706	arg1	strength					708:715	the tensile strength	696:715	the tensile strength (TS)	696:720	The results revealed that incorporation of GSO-NE at 6 % concentration decreased the tensile strength (TS) and puncture force (PF) significantly (p < 0.05).					
36990416	9	61	theme	Gram-negative	1531:1543	arg1	bacteria					1545:1552	both Gram-positive and Gram-negative bacteria	1508:1552	both Gram-positive and Gram-negative bacteria	1508:1552	Ge/SA/GSO-NE films were found to be effective against both Gram-positive and Gram-negative bacteria.					
36990416	2	62	theme	different	421:429	arg1	levels					431:436	different levels	421:436	different levels (2, 4, and 6 %) of nanoemulsioned GSO	421:474	In this study, ultrasonic treatment was used to prepare GSO-NE, and different levels (2, 4, and 6 %) of nanoemulsioned GSO were incorporated into gelatin (Ge)/sodium alginate (SA)-based films to produce films with improved physical and antibacterial properties.					
36990416	2	62	theme	different	421:429	arg1	%					451:451	2, 4, and 6 %	439:451	2, 4, and 6 %	439:451	In this study, ultrasonic treatment was used to prepare GSO-NE, and different levels (2, 4, and 6 %) of nanoemulsioned GSO were incorporated into gelatin (Ge)/sodium alginate (SA)-based films to produce films with improved physical and antibacterial properties.					
36990416	1	63	theme	physicochemical	284:298	arg1	properties					318:327	the physicochemical and antimicrobial properties	280:327	the physicochemical and antimicrobial properties of the resulting films	280:350	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	4	64	theme	whiteness	776:784	arg1	WI					793:794	WI	793:794	WI	793:794	The whiteness index (WI) of the films decreased from 63.4 to 47.79, while the total color change (ΔE) increased significantly (p < 0.05) with the increase in GSO-NE concentration.					
36990416	4	64	theme	whiteness	776:784	arg1	index					786:790	The whiteness index	772:790	The whiteness index (WI) of the films	772:808	The whiteness index (WI) of the films decreased from 63.4 to 47.79, while the total color change (ΔE) increased significantly (p < 0.05) with the increase in GSO-NE concentration.					
36990416	4	65	dep	increased	874:882	arg1	p < 0.05					899:906	p < 0.05	899:906	p < 0.05	899:906	The whiteness index (WI) of the films decreased from 63.4 to 47.79, while the total color change (ΔE) increased significantly (p < 0.05) with the increase in GSO-NE concentration.					
36990416	2	66	used	used	393:396	arg2	treatment					379:387	ultrasonic treatment	368:387	ultrasonic treatment	368:387	In this study, ultrasonic treatment was used to prepare GSO-NE, and different levels (2, 4, and 6 %) of nanoemulsioned GSO were incorporated into gelatin (Ge)/sodium alginate (SA)-based films to produce films with improved physical and antibacterial properties.					
36990416	7	67	theme	solubility	1330:1339	arg1	%					1346:1346	moisture content (MC) %, and water solubility (WS) %	1295:1346	%	1346:1346	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	7	67	theme	solubility	1330:1339	arg1	permeability					1275:1286	the water vapor permeability	1259:1286	the water vapor permeability (WVP)	1259:1292	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	10	68	theme	food	1652:1655	arg1	packaging					1657:1665	food packaging	1652:1665	food packaging	1652:1665	The prepared active films containing GSO-NE had a high potential for preventing food spoilage in food packaging.					
36990416	7	69	theme	water	1324:1328	arg1	%					1346:1346	moisture content (MC) %, and water solubility (WS) %	1295:1346	%	1346:1346	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	7	69	theme	water	1324:1328	arg1	permeability					1275:1286	the water vapor permeability	1259:1286	the water vapor permeability (WVP)	1259:1292	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	1	70	theme	present	131:137	arg1	study					139:143	The present study	127:143	The present study	127:143	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	7	71	from	concentrations	1234:1247	arg1	incorporation					1197:1209	The incorporation	1193:1209	The incorporation of GSO-NE at 4 and 6 % concentrations	1193:1247	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	5	72	theme	Ge/SA-based	1078:1088	arg1	films					1090:1094	Ge/SA-based films	1078:1094	Ge/SA-based films	1078:1094	Thermogravimetric analysis (TGA) results showed that GSO-NE at different concentrations had improved the thermal stability of Ge/SA-based films.					
36990416	4	73	dep	47.79	833:837	arg1	to					830:831	to	830:831	to	830:831	The whiteness index (WI) of the films decreased from 63.4 to 47.79, while the total color change (ΔE) increased significantly (p < 0.05) with the increase in GSO-NE concentration.					
36990416	7	74	theme	GSO-NE	1214:1219	arg1	incorporation					1197:1209	The incorporation	1193:1209	The incorporation of GSO-NE at 4 and 6 % concentrations	1193:1247	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	10	75	from	spoilage	1640:1647	arg1	packaging					1657:1665	food packaging	1652:1665	food packaging	1652:1665	The prepared active films containing GSO-NE had a high potential for preventing food spoilage in food packaging.					
36990416	4	76	theme	GSO-NE	930:935	arg1	concentration					937:949	GSO-NE concentration	930:949	GSO-NE concentration	930:949	The whiteness index (WI) of the films decreased from 63.4 to 47.79, while the total color change (ΔE) increased significantly (p < 0.05) with the increase in GSO-NE concentration.					
36990416	4	77	from	increase	918:925	arg1	concentration					937:949	GSO-NE concentration	930:949	GSO-NE concentration	930:949	The whiteness index (WI) of the films decreased from 63.4 to 47.79, while the total color change (ΔE) increased significantly (p < 0.05) with the increase in GSO-NE concentration.					
36990416	0	78	theme	antibacterial	62:74	arg1	properties					76:85	physicochemical and antibacterial properties	42:85	physicochemical and antibacterial properties of gelatin‑sodium alginate film blends	42:124	Effects of grape seed oil nanoemulsion on physicochemical and antibacterial properties of gelatin‑sodium alginate film blends.					
36990416	7	79	dep	significantly	1348:1360	arg1	p < 0.05					1363:1370	p < 0.05	1363:1370	p < 0.05	1363:1370	The incorporation of GSO-NE at 4 and 6 % concentrations decreased the water vapor permeability (WVP), moisture content (MC) %, and water solubility (WS) % significantly (p < 0.05).					
36990416	10	80	theme	prepared	1559:1566	arg1	films					1575:1579	The prepared active films	1555:1579	The prepared active films containing GSO-NE	1555:1597	The prepared active films containing GSO-NE had a high potential for preventing food spoilage in food packaging.					
36990416	1	81	theme	antimicrobial	304:316	arg1	properties					318:327	the physicochemical and antimicrobial properties	280:327	the physicochemical and antimicrobial properties of the resulting films	280:350	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	1	82	from	impact	167:172	arg1	properties					318:327	the physicochemical and antimicrobial properties	280:327	the physicochemical and antimicrobial properties of the resulting films	280:350	The present study aimed to evaluate the impact of incorporating grape seed oil (GSO) nanoemulsion (NE) at varying concentrations into the film matrix on the physicochemical and antimicrobial properties of the resulting films.					
36990416	9	83	theme	Gram-positive	1513:1525	arg1	bacteria					1545:1552	both Gram-positive and Gram-negative bacteria	1508:1552	both Gram-positive and Gram-negative bacteria	1508:1552	Ge/SA/GSO-NE films were found to be effective against both Gram-positive and Gram-negative bacteria.					
36990416	8	84	theme	hydrophobic	1404:1414	arg1	surfaces					1416:1423	hydrophobic surfaces	1404:1423	hydrophobic surfaces	1404:1423	All composite films exhibited hydrophobic surfaces with contact angles θ > 90°.					
36773870	0	0	theme	wastewater	87:96	arg1	purification					98:109	wastewater purification	87:109	wastewater purification	87:109	Preparation of cellulose-based porous adsorption materials derived from corn straw for wastewater purification.					
36773870	4	1	dep	durability	878:887	arg1	%					893:893	70 %	890:893	70 % of adsorption efficiency after 5 cycles	890:933	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	4	1	dep	durability	878:887	arg1	efficiency					909:918	adsorption efficiency	898:918	adsorption efficiency after 5 cycles	898:933	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	1	2	theme	heavy	156:160	arg1	contamination					172:184	heavy metal ion contamination	156:184	heavy metal ion contamination	156:184	Various methods have been used to cope with heavy metal ion contamination in wastewater, which caused serious hazards to ecological and human health.					
36773870	1	3	theme	human	248:252	arg1	health					254:259	ecological and human health	233:259	ecological and human health	233:259	Various methods have been used to cope with heavy metal ion contamination in wastewater, which caused serious hazards to ecological and human health.					
36773870	1	4	theme	metal	162:166	arg1	contamination					172:184	heavy metal ion contamination	156:184	heavy metal ion contamination	156:184	Various methods have been used to cope with heavy metal ion contamination in wastewater, which caused serious hazards to ecological and human health.					
36773870	4	5	theme	straw	601:605	arg1	substrate					607:615	straw substrate	601:615	straw substrate	601:615	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	7	6	theme	adsorbents	1196:1205	arg1	manufacture					1157:1167	the manufacture	1153:1167	the manufacture of efficient biomass-based adsorbents	1153:1205	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	4	7	theme	adsorption	748:757	arg1	behavior					759:766	rapid adsorption behavior	742:766	rapid adsorption behavior (within 180 min achieve adsorption equilibrium)	742:814	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	7	8	theme	sustainable	1129:1139	arg1	pathway					1141:1147	a sustainable pathway	1127:1147	a sustainable pathway for the manufacture of efficient biomass-based adsorbents	1127:1205	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	4	9	theme	various	721:727	arg1	ions					735:738	various metal ions	721:738	various metal ions	721:738	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	4	9	theme	various	721:727	arg1	universality					707:718	universality	707:718	universality (various metal ions)	707:739	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	7	10	theme	efficient	1172:1180	arg1	adsorbents					1196:1205	efficient biomass-based adsorbents	1172:1205	efficient biomass-based adsorbents	1172:1205	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	7	11	theme	ions	1308:1311	arg1	treatment					1283:1291	the treatment	1279:1291	the treatment of heavy metal ions	1279:1311	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	3	12	theme	corn	559:562	arg1	straw					564:568	delignified corn straw	547:568	delignified corn straw	547:568	Herein, a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property was successfully prepared by covalently cross-linking polyethyleneimine on delignified corn straw.					
36773870	1	13	theme	ion	168:170	arg1	contamination					172:184	heavy metal ion contamination	156:184	heavy metal ion contamination	156:184	Various methods have been used to cope with heavy metal ion contamination in wastewater, which caused serious hazards to ecological and human health.					
36773870	7	14	theme	biomass-based	1182:1194	arg1	adsorbents					1196:1205	efficient biomass-based adsorbents	1172:1205	efficient biomass-based adsorbents	1172:1205	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	3	15	theme	cellulose-based	400:414	arg1	PEI-PCS					437:443	a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS)	376:444	a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property	376:470	Herein, a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property was successfully prepared by covalently cross-linking polyethyleneimine on delignified corn straw.					
36773870	4	16	theme	substrate	607:615	arg1	ability					635:641	the chelating ability	621:641	the chelating ability of amino group to metal ions	621:670	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	4	16	theme	substrate	607:615	arg1	porosity					589:596	the porosity	585:596	the porosity of straw substrate	585:615	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	2	17	theme	economical	302:311	arg1	methods					327:333	the most frequent, economical and effective methods	283:333	the most frequent, economical and effective methods for removing these contaminants	283:365	Adsorption is one of the most frequent, economical and effective methods for removing these contaminants.					
36773870	3	18	theme	adsorbent	426:434	arg1	PEI-PCS					437:443	a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS)	376:444	a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property	376:470	Herein, a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property was successfully prepared by covalently cross-linking polyethyleneimine on delignified corn straw.					
36773870	3	19	theme	porous	378:383	arg1	PEI-PCS					437:443	a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS)	376:444	a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property	376:470	Herein, a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property was successfully prepared by covalently cross-linking polyethyleneimine on delignified corn straw.					
36773870	1	20	used	used	138:141	arg2	methods					120:126	Various methods	112:126	Various methods	112:126	Various methods have been used to cope with heavy metal ion contamination in wastewater, which caused serious hazards to ecological and human health.					
36773870	4	21	theme	as-prepared	677:687	arg1	PEI-PCS					689:695	the as-prepared PEI-PCS	673:695	the as-prepared PEI-PCS	673:695	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	0	22	theme	porous	31:36	arg1	materials					49:57	cellulose-based porous adsorption materials	15:57	cellulose-based porous adsorption materials	15:57	Preparation of cellulose-based porous adsorption materials derived from corn straw for wastewater purification.					
36773870	4	23	theme	rapid	742:746	arg1	behavior					759:766	rapid adsorption behavior	742:766	rapid adsorption behavior (within 180 min achieve adsorption equilibrium)	742:814	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	2	24	theme	frequent	292:299	arg1	methods					327:333	the most frequent, economical and effective methods	283:333	the most frequent, economical and effective methods for removing these contaminants	283:365	Adsorption is one of the most frequent, economical and effective methods for removing these contaminants.					
36773870	7	25	theme	metal	1302:1306	arg1	ions					1308:1311	heavy metal ions	1296:1311	heavy metal ions	1296:1311	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	0	26	theme	cellulose-based	15:29	arg1	materials					49:57	cellulose-based porous adsorption materials	15:57	cellulose-based porous adsorption materials	15:57	Preparation of cellulose-based porous adsorption materials derived from corn straw for wastewater purification.					
36773870	4	27	theme	adsorption	792:801	arg1	equilibrium					803:813	adsorption equilibrium	792:813	adsorption equilibrium	792:813	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	4	28	theme	adsorption	898:907	arg1	efficiency					909:918	adsorption efficiency	898:918	adsorption efficiency after 5 cycles	898:933	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	6	29	theme	adsorption	1065:1074	arg1	mechanism					1076:1084	the adsorption mechanism	1061:1084	the adsorption mechanism	1061:1084	Lastly, the adsorption mechanism was also elucidated.					
36773870	4	30	theme	group	652:656	arg1	ability					635:641	the chelating ability	621:641	the chelating ability of amino group to metal ions	621:670	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	4	30	theme	group	652:656	arg1	porosity					589:596	the porosity	585:596	the porosity of straw substrate	585:615	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	0	31	theme	materials	49:57	arg1	Preparation					0:10	Preparation	0:10	Preparation of cellulose-based porous adsorption materials	0:57	Preparation of cellulose-based porous adsorption materials derived from corn straw for wastewater purification.					
36773870	4	32	theme	metal	729:733	arg1	ions					735:738	various metal ions	721:738	various metal ions	721:738	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	4	32	theme	metal	729:733	arg1	universality					707:718	universality	707:718	universality (various metal ions)	707:739	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	4	33	theme	efficiency	909:918	arg1	%					893:893	70 %	890:893	70 % of adsorption efficiency after 5 cycles	890:933	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	4	33	theme	efficiency	909:918	arg1	efficiency					909:918	adsorption efficiency	898:918	adsorption efficiency after 5 cycles	898:933	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	4	34	theme	metal	661:665	arg1	ions					667:670	metal ions	661:670	metal ions	661:670	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	7	35	theme	heavy	1296:1300	arg1	ions					1308:1311	heavy metal ions	1296:1311	heavy metal ions	1296:1311	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	4	36	dep	capacity	833:840	arg1	85.47 mg g-1					843:854	85.47 mg g-1	843:854	85.47 mg g-1 for Cu(II)	843:865	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	0	37	theme	adsorption	38:47	arg1	materials					49:57	cellulose-based porous adsorption materials	15:57	cellulose-based porous adsorption materials	15:57	Preparation of cellulose-based porous adsorption materials derived from corn straw for wastewater purification.					
36773870	3	38	theme	composite	416:424	arg1	PEI-PCS					437:443	a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS)	376:444	a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property	376:470	Herein, a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property was successfully prepared by covalently cross-linking polyethyleneimine on delignified corn straw.					
36773870	4	39	theme	adsorption	822:831	arg1	capacity					833:840	high adsorption capacity	817:840	high adsorption capacity (85.47 mg g-1 for Cu(II))	817:866	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	1	40	theme	serious	214:220	arg1	hazards					222:228	serious hazards	214:228	serious hazards	214:228	Various methods have been used to cope with heavy metal ion contamination in wastewater, which caused serious hazards to ecological and human health.					
36773870	7	41	theme	corn	1240:1243	arg1	straw					1245:1249	functionalized corn straw	1225:1249	functionalized corn straw	1225:1249	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	7	41	theme	corn	1240:1243	arg1	material					1266:1273	a promising material	1254:1273	a promising material for the treatment of heavy metal ions	1254:1311	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	3	42	theme	amino-rich	389:398	arg1	PEI-PCS					437:443	a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS)	376:444	a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property	376:470	Herein, a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property was successfully prepared by covalently cross-linking polyethyleneimine on delignified corn straw.					
36773870	4	43	theme	good	873:876	arg1	durability					878:887	good durability	873:887	good durability (70 % of adsorption efficiency after 5 cycles)	873:934	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	4	44	dep	180 min	776:782	arg1	achieve					784:790	achieve	784:790	achieve adsorption equilibrium	784:813	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	2	45	theme	methods	327:333	arg1	methods					327:333	the most frequent, economical and effective methods	283:333	the most frequent, economical and effective methods for removing these contaminants	283:365	Adsorption is one of the most frequent, economical and effective methods for removing these contaminants.					
36773870	2	45	theme	methods	327:333	arg1	one					276:278	one	276:278	one	276:278	Adsorption is one of the most frequent, economical and effective methods for removing these contaminants.					
36773870	3	46	with	PEI-PCS	437:443	arg1	property					463:470	anisotropic property	451:470	anisotropic property	451:470	Herein, a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property was successfully prepared by covalently cross-linking polyethyleneimine on delignified corn straw.					
36773870	5	47	theme	Langmuir	1027:1034	arg1	models					1045:1050	the Langmuir isotherm models	1023:1050	the Langmuir isotherm models	1023:1050	In addition, the adsorption process was conformed to pseudo-second-order dynamics and the Langmuir isotherm models.					
36773870	5	48	theme	adsorption	954:963	arg1	process					965:971	the adsorption process	950:971	the adsorption process	950:971	In addition, the adsorption process was conformed to pseudo-second-order dynamics and the Langmuir isotherm models.					
36773870	5	49	theme	pseudo-second-order	990:1008	arg1	dynamics					1010:1017	pseudo-second-order dynamics	990:1017	pseudo-second-order dynamics	990:1017	In addition, the adsorption process was conformed to pseudo-second-order dynamics and the Langmuir isotherm models.					
36773870	2	50	theme	effective	317:325	arg1	methods					327:333	the most frequent, economical and effective methods	283:333	the most frequent, economical and effective methods for removing these contaminants	283:365	Adsorption is one of the most frequent, economical and effective methods for removing these contaminants.					
36773870	1	51	theme	Various	112:118	arg1	methods					120:126	Various methods	112:126	Various methods	112:126	Various methods have been used to cope with heavy metal ion contamination in wastewater, which caused serious hazards to ecological and human health.					
36773870	5	52	theme	isotherm	1036:1043	arg1	models					1045:1050	the Langmuir isotherm models	1023:1050	the Langmuir isotherm models	1023:1050	In addition, the adsorption process was conformed to pseudo-second-order dynamics and the Langmuir isotherm models.					
36773870	7	53	theme	promising	1256:1264	arg1	straw					1245:1249	functionalized corn straw	1225:1249	functionalized corn straw	1225:1249	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	7	53	theme	promising	1256:1264	arg1	material					1266:1273	a promising material	1254:1273	a promising material for the treatment of heavy metal ions	1254:1311	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	4	54	theme	chelating	625:633	arg1	ability					635:641	the chelating ability	621:641	the chelating ability of amino group to metal ions	621:670	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	7	55	theme	functionalized	1225:1238	arg1	straw					1245:1249	functionalized corn straw	1225:1249	functionalized corn straw	1225:1249	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	7	55	theme	functionalized	1225:1238	arg1	material					1266:1273	a promising material	1254:1273	a promising material for the treatment of heavy metal ions	1254:1311	This study provides a sustainable pathway for the manufacture of efficient biomass-based adsorbents and confirms that functionalized corn straw is a promising material for the treatment of heavy metal ions.					
36773870	3	56	theme	anisotropic	451:461	arg1	property					463:470	anisotropic property	451:470	anisotropic property	451:470	Herein, a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property was successfully prepared by covalently cross-linking polyethyleneimine on delignified corn straw.					
36773870	4	57	theme	high	817:820	arg1	capacity					833:840	high adsorption capacity	817:840	high adsorption capacity (85.47 mg g-1 for Cu(II))	817:866	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	4	58	theme	amino	646:650	arg1	group					652:656	amino group	646:656	amino group	646:656	Combined with the porosity of straw substrate and the chelating ability of amino group to metal ions, the as-prepared PEI-PCS exhibited universality (various metal ions), rapid adsorption behavior (within 180 min achieve adsorption equilibrium), high adsorption capacity (85.47 mg g-1 for Cu(II)), and good durability (70 % of adsorption efficiency after 5 cycles).					
36773870	1	59	theme	ecological	233:242	arg1	health					254:259	ecological and human health	233:259	ecological and human health	233:259	Various methods have been used to cope with heavy metal ion contamination in wastewater, which caused serious hazards to ecological and human health.					
36773870	0	60	theme	corn	72:75	arg1	straw					77:81	corn straw	72:81	corn straw for wastewater purification	72:109	Preparation of cellulose-based porous adsorption materials derived from corn straw for wastewater purification.					
36773870	3	61	theme	delignified	547:557	arg1	straw					564:568	delignified corn straw	547:568	delignified corn straw	547:568	Herein, a porous and amino-rich cellulose-based composite adsorbent (PEI-PCS) with anisotropic property was successfully prepared by covalently cross-linking polyethyleneimine on delignified corn straw.					
35572846	10	0	from	evidence	1523:1530	arg1	formulation					1572:1582	the commercial palivizumab formulation	1545:1582	the commercial palivizumab formulation using DMB labelling	1545:1602	Next, we provide the first experimental evidence of Neu5Gc in the commercial palivizumab formulation using DMB labelling.					
35572846	5	1	theme	profile	656:662	arg1	glycosylation					676:688	glycosylation	676:688	especially glycosylation	665:688	Therefore, a thorough estimation of their PTM profile, especially glycosylation, is relevant.					
35572846	5	1	theme	profile	656:662	arg1	relevant					694:701	relevant	694:701	relevant	694:701	Therefore, a thorough estimation of their PTM profile, especially glycosylation, is relevant.					
35572846	5	1	theme	profile	656:662	arg1	estimation					632:641	a thorough estimation	621:641	a thorough estimation of their PTM profile	621:662	Therefore, a thorough estimation of their PTM profile, especially glycosylation, is relevant.					
35572846	10	2	theme	palivizumab	1560:1570	arg1	formulation					1572:1582	the commercial palivizumab formulation	1545:1582	the commercial palivizumab formulation using DMB labelling	1545:1602	Next, we provide the first experimental evidence of Neu5Gc in the commercial palivizumab formulation using DMB labelling.					
35572846	2	3	from	infections	349:358	arg1	infants					382:388	infants	382:388	infants	382:388	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	6	4	theme	HILIC	794:798	arg1	chromatography					800:813	HILIC chromatography	794:813	HILIC chromatography	794:813	Here, we describe the intact and released glycan analysis of palivizumab (Synagis®) using HILIC chromatography and mass spectrometry.					
35572846	13	5	theme	release	2055:2061	arg1	analysis					2039:2046	the analysis	2035:2046	the analysis of the release of N-glycan, sialic acid, and monosaccharide composition	2035:2118	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	2	6	theme	negative-sense	288:301	arg1	virus					307:311	another highly contagious negative-sense RNA virus	262:311	another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants	262:388	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	9	7	theme	palivizumab.Release	1189:1207	arg1	analysis					1216:1223	palivizumab.Release glycan analysis	1189:1223	palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans	1189:1303	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	2	8	theme	respiratory	331:341	arg1	infections					349:358	lower respiratory tract infections	325:358	lower respiratory tract infections	325:358	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	1	9	theme	COVID-19	206:213	arg1	pandemic					215:222	the ongoing COVID-19 pandemic	194:222	the ongoing COVID-19 pandemic	194:222	Viral infections are progressively becoming a global health burden, as witnessed in the ongoing COVID-19 pandemic.					
35572846	12	10	theme	intact	1848:1853	arg1	approach					1881:1888	the intact glycoprotein/glycopeptide approach	1844:1888	the intact glycoprotein/glycopeptide approach	1844:1888	The findings of the study highlight the efficiency of the release glycan method in providing a correct measure of the total palivizumab glycan pool compared to the intact glycoprotein/glycopeptide approach.					
35572846	16	11	theme	online	2546:2551	arg1	version					2553:2559	The online version	2542:2559	Supplementary Information The online version	2516:2559	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00086-1.					
35572846	9	12	with	forms	1426:1430	arg1	resolution					1450:1459	the superior resolution	1437:1459	the superior resolution of isomeric G1F/G1F'	1437:1480	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	9	13	theme	analysis	1216:1223	arg1	chain					1180:1184	the heavy chain	1170:1184	the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans	1170:1303	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	16	14	contain	contains	2561:2568	arg1	Information					2530:2540	Supplementary Information	2516:2540	Supplementary Information The online version	2516:2559	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00086-1.					
35572846	16	14	contain	contains	2561:2568	arg2	material					2584:2591	supplementary material	2570:2591	supplementary material available at 10.1007/s42485-022-00086-1	2570:2631	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00086-1.					
35572846	12	15	theme	palivizumab	1808:1818	arg1	pool					1827:1830	the total palivizumab glycan pool	1798:1830	the total palivizumab glycan pool	1798:1830	The findings of the study highlight the efficiency of the release glycan method in providing a correct measure of the total palivizumab glycan pool compared to the intact glycoprotein/glycopeptide approach.					
35572846	12	16	theme	pool	1827:1830	arg1	measure					1787:1793	a correct measure	1777:1793	a correct measure of the total palivizumab glycan pool	1777:1830	The findings of the study highlight the efficiency of the release glycan method in providing a correct measure of the total palivizumab glycan pool compared to the intact glycoprotein/glycopeptide approach.					
35572846	15	17	theme	cost-effective	2254:2267	arg1	immunoprophylaxis					2269:2285	cost-effective immunoprophylaxis	2254:2285	cost-effective immunoprophylaxis	2254:2285	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	13	18	theme	parallel	1996:2003	arg1	use					2005:2007	the parallel use	1992:2007	the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition	1992:2118	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	9	19	theme	glycan	1262:1267	arg1	profile					1269:1275	a typical glycan profile	1252:1275	a typical glycan profile consisting of major glycans	1252:1303	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	7	20	theme	palivizumab	970:980	arg1	spectra					939:945	deconvoluted MS spectra	923:945	deconvoluted MS spectra of intact glycosylated palivizumab	923:980	We detected five glycoforms (Man5/G0FB, G0F/G1F, G1F/G1F, G0FB/G0FB, and G2F/G2F) in deconvoluted MS spectra of intact glycosylated palivizumab.					
35572846	1	21	theme	health	163:168	arg1	burden					170:175	a global health burden	154:175	a global health burden	154:175	Viral infections are progressively becoming a global health burden, as witnessed in the ongoing COVID-19 pandemic.					
35572846	4	22	dep	nature	550:555	arg1	the					546:548	the	546:548	the	546:548	The virus neutralization efficacy often depends on the nature and abundance of the glycoforms in therapeutic mAbs.					
35572846	7	23	theme	intact	950:955	arg1	palivizumab					970:980	intact glycosylated palivizumab	950:980	intact glycosylated palivizumab	950:980	We detected five glycoforms (Man5/G0FB, G0F/G1F, G1F/G1F, G0FB/G0FB, and G2F/G2F) in deconvoluted MS spectra of intact glycosylated palivizumab.					
35572846	4	24	theme	virus	499:503	arg1	efficacy					520:527	The virus neutralization efficacy	495:527	The virus neutralization efficacy	495:527	The virus neutralization efficacy often depends on the nature and abundance of the glycoforms in therapeutic mAbs.					
35572846	16	25	from	10.1007/s42485-022-00086-1	2606:2631	arg1	available					2593:2601	available	2593:2601	available	2593:2601	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00086-1.					
35572846	6	26	theme	glycan	746:751	arg1	analysis					753:760	the intact and released glycan analysis	722:760	the intact and released glycan analysis of palivizumab (Synagis®)	722:786	Here, we describe the intact and released glycan analysis of palivizumab (Synagis®) using HILIC chromatography and mass spectrometry.					
35572846	9	27	theme	major	1291:1295	arg1	glycans					1297:1303	major glycans	1291:1303	major glycans	1291:1303	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	8	28	theme	peptide	1002:1008	arg1	mapping					987:993	The mapping	983:993	The mapping of the peptide and glycopeptides using LC-ESI-MS	983:1042	The mapping of the peptide and glycopeptides using LC-ESI-MS led to the detection of associated PTMs and the direct identification of a glycopeptide, GlcNAc3Man2.					
35572846	11	29	theme	monosaccharide	1619:1632	arg1	composition					1634:1644	The estimated monosaccharide composition	1605:1644	The estimated monosaccharide composition	1605:1644	The estimated monosaccharide composition was consistent with previous studies.					
35572846	11	29	theme	monosaccharide	1619:1632	arg1	consistent					1650:1659	consistent	1650:1659	consistent	1650:1659	The estimated monosaccharide composition was consistent with previous studies.					
35572846	15	30	theme	next-generation	2329:2343	arg1	variants					2345:2352	next-generation variants	2329:2352	next-generation variants	2329:2352	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	15	30	theme	next-generation	2329:2343	arg1	MEDI8897					2378:2385	MEDI8897	2378:2385	MEDI8897	2378:2385	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	8	31	theme	glycopeptides	1014:1026	arg1	mapping					987:993	The mapping	983:993	The mapping of the peptide and glycopeptides using LC-ESI-MS	983:1042	The mapping of the peptide and glycopeptides using LC-ESI-MS led to the detection of associated PTMs and the direct identification of a glycopeptide, GlcNAc3Man2.					
35572846	7	32	theme	deconvoluted	923:934	arg1	spectra					939:945	deconvoluted MS spectra	923:945	deconvoluted MS spectra of intact glycosylated palivizumab	923:980	We detected five glycoforms (Man5/G0FB, G0F/G1F, G1F/G1F, G0FB/G0FB, and G2F/G2F) in deconvoluted MS spectra of intact glycosylated palivizumab.					
35572846	0	33	theme	palivizumab	55:65	arg1	glycoprofiling					37:50	glycoprofiling	37:50	glycoprofiling of palivizumab by UHPLC-RPLC/HILIC and mass spectrometry	37:107	Post-translational modifications and glycoprofiling of palivizumab by UHPLC-RPLC/HILIC and mass spectrometry.					
35572846	0	33	theme	palivizumab	55:65	arg1	modifications					19:31	Post-translational modifications	0:31	Post-translational modifications	0:31	Post-translational modifications and glycoprofiling of palivizumab by UHPLC-RPLC/HILIC and mass spectrometry.					
35572846	14	34	theme	quality	2157:2163	arg1	testing					2165:2171	quick quality testing	2151:2171	quick quality testing	2151:2171	This approach is suitable for quick quality testing and market surveillance of therapeutic mAbs.					
35572846	0	35	theme	Post-translational	0:17	arg1	modifications					19:31	Post-translational modifications	0:31	Post-translational modifications	0:31	Post-translational modifications and glycoprofiling of palivizumab by UHPLC-RPLC/HILIC and mass spectrometry.					
35572846	13	36	theme	composition	2108:2118	arg1	release					2055:2061	the release	2051:2061	the release of N-glycan, sialic acid, and monosaccharide composition	2051:2118	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	13	37	theme	glycoprofile	1961:1972	arg1	assessment					1974:1983	a more comprehensive glycoprofile assessment	1940:1983	a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition	1940:2118	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	1	38	dep	burden	170:175	arg1	witnessed					181:189	witnessed	181:189	witnessed in the ongoing COVID-19 pandemic	181:222	Viral infections are progressively becoming a global health burden, as witnessed in the ongoing COVID-19 pandemic.					
35572846	3	39	theme	humanized	426:434	arg1	mAb					457:459	mAb	457:459	mAb	457:459	Palivizumab (Synagis®) is the only humanized monoclonal antibody (mAb) approved by the FDA against RSV.					
35572846	3	39	theme	humanized	426:434	arg1	antibody					447:454	the only humanized monoclonal antibody	417:454	the only humanized monoclonal antibody (mAb) approved by the FDA against RSV	417:492	Palivizumab (Synagis®) is the only humanized monoclonal antibody (mAb) approved by the FDA against RSV.					
35572846	3	39	theme	humanized	426:434	arg1	Palivizumab					391:401	Palivizumab	391:401	Palivizumab (Synagis®)	391:412	Palivizumab (Synagis®) is the only humanized monoclonal antibody (mAb) approved by the FDA against RSV.					
35572846	3	40	theme	approved	462:469	arg1	mAb					457:459	mAb	457:459	mAb	457:459	Palivizumab (Synagis®) is the only humanized monoclonal antibody (mAb) approved by the FDA against RSV.					
35572846	3	40	theme	approved	462:469	arg1	antibody					447:454	the only humanized monoclonal antibody	417:454	the only humanized monoclonal antibody (mAb) approved by the FDA against RSV	417:492	Palivizumab (Synagis®) is the only humanized monoclonal antibody (mAb) approved by the FDA against RSV.					
35572846	3	40	theme	approved	462:469	arg1	Palivizumab					391:401	Palivizumab	391:401	Palivizumab (Synagis®)	391:412	Palivizumab (Synagis®) is the only humanized monoclonal antibody (mAb) approved by the FDA against RSV.					
35572846	10	41	theme	experimental	1510:1521	arg1	evidence					1523:1530	the first experimental evidence	1500:1530	the first experimental evidence of Neu5Gc in the commercial palivizumab formulation using DMB labelling	1500:1602	Next, we provide the first experimental evidence of Neu5Gc in the commercial palivizumab formulation using DMB labelling.					
35572846	13	42	theme	acid	2083:2086	arg1	release					2055:2061	the release	2051:2061	the release of N-glycan, sialic acid, and monosaccharide composition	2051:2118	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	12	43	theme	release	1742:1748	arg1	method					1757:1762	the release glycan method	1738:1762	the release glycan method	1738:1762	The findings of the study highlight the efficiency of the release glycan method in providing a correct measure of the total palivizumab glycan pool compared to the intact glycoprotein/glycopeptide approach.					
35572846	10	44	theme	commercial	1549:1558	arg1	formulation					1572:1582	the commercial palivizumab formulation	1545:1582	the commercial palivizumab formulation using DMB labelling	1545:1602	Next, we provide the first experimental evidence of Neu5Gc in the commercial palivizumab formulation using DMB labelling.					
35572846	8	45	theme	associated	1068:1077	arg1	PTMs					1079:1082	associated PTMs	1068:1082	associated PTMs	1068:1082	The mapping of the peptide and glycopeptides using LC-ESI-MS led to the detection of associated PTMs and the direct identification of a glycopeptide, GlcNAc3Man2.					
35572846	12	46	theme	study	1704:1708	arg1	findings					1688:1695	The findings	1684:1695	The findings of the study	1684:1708	The findings of the study highlight the efficiency of the release glycan method in providing a correct measure of the total palivizumab glycan pool compared to the intact glycoprotein/glycopeptide approach.					
35572846	9	47	theme	isomeric	1464:1471	arg1	G1F/G1F					1473:1479	isomeric G1F/G1F'	1464:1480	isomeric G1F/G1F'	1464:1480	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	14	48	theme	therapeutic	2200:2210	arg1	mAbs					2212:2215	therapeutic mAbs	2200:2215	therapeutic mAbs	2200:2215	This approach is suitable for quick quality testing and market surveillance of therapeutic mAbs.					
35572846	15	49	theme	well-perceived	2230:2243	arg1	need					2245:2248	a well-perceived need	2228:2248	a well-perceived need for cost-effective immunoprophylaxis	2228:2285	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	2	50	theme	Respiratory	225:235	arg1	RSV					254:256	RSV	254:256	RSV	254:256	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	2	50	theme	Respiratory	225:235	arg1	Virus					247:251	Respiratory Syncytial Virus	225:251	Respiratory Syncytial Virus (RSV)	225:257	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	6	51	theme	released	737:744	arg1	analysis					753:760	the intact and released glycan analysis	722:760	the intact and released glycan analysis of palivizumab (Synagis®)	722:786	Here, we describe the intact and released glycan analysis of palivizumab (Synagis®) using HILIC chromatography and mass spectrometry.					
35572846	8	52	theme	direct	1092:1097	arg1	identification					1099:1112	the direct identification	1088:1112	the direct identification of a glycopeptide	1088:1130	The mapping of the peptide and glycopeptides using LC-ESI-MS led to the detection of associated PTMs and the direct identification of a glycopeptide, GlcNAc3Man2.					
35572846	6	53	theme	intact	726:731	arg1	analysis					753:760	the intact and released glycan analysis	722:760	the intact and released glycan analysis of palivizumab (Synagis®)	722:786	Here, we describe the intact and released glycan analysis of palivizumab (Synagis®) using HILIC chromatography and mass spectrometry.					
35572846	10	54	theme	DMB	1590:1592	arg1	labelling					1594:1602	DMB labelling	1590:1602	DMB labelling	1590:1602	Next, we provide the first experimental evidence of Neu5Gc in the commercial palivizumab formulation using DMB labelling.					
35572846	5	55	theme	PTM	652:654	arg1	profile					656:662	their PTM profile	646:662	their PTM profile	646:662	Therefore, a thorough estimation of their PTM profile, especially glycosylation, is relevant.					
35572846	8	56	theme	glycopeptide	1119:1130	arg1	detection					1055:1063	the detection	1051:1063	the detection of associated PTMs	1051:1082	The mapping of the peptide and glycopeptides using LC-ESI-MS led to the detection of associated PTMs and the direct identification of a glycopeptide, GlcNAc3Man2.					
35572846	8	56	theme	glycopeptide	1119:1130	arg1	GlcNAc3Man2					1133:1143	GlcNAc3Man2	1133:1143	GlcNAc3Man2	1133:1143	The mapping of the peptide and glycopeptides using LC-ESI-MS led to the detection of associated PTMs and the direct identification of a glycopeptide, GlcNAc3Man2.					
35572846	8	56	theme	glycopeptide	1119:1130	arg1	identification					1099:1112	the direct identification	1088:1112	the direct identification of a glycopeptide	1088:1130	The mapping of the peptide and glycopeptides using LC-ESI-MS led to the detection of associated PTMs and the direct identification of a glycopeptide, GlcNAc3Man2.					
35572846	4	57	theme	glycoforms	578:587	arg1	abundance					561:569	abundance	561:569	abundance	561:569	The virus neutralization efficacy often depends on the nature and abundance of the glycoforms in therapeutic mAbs.					
35572846	4	57	theme	glycoforms	578:587	arg1	nature					550:555	nature	550:555	nature	550:555	The virus neutralization efficacy often depends on the nature and abundance of the glycoforms in therapeutic mAbs.					
35572846	16	58	dep	Information	2530:2540	arg1	version					2553:2559	The online version	2542:2559	Supplementary Information The online version	2516:2559	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00086-1.					
35572846	12	59	theme	glycoprotein/glycopeptide	1855:1879	arg1	approach					1881:1888	the intact glycoprotein/glycopeptide approach	1844:1888	the intact glycoprotein/glycopeptide approach	1844:1888	The findings of the study highlight the efficiency of the release glycan method in providing a correct measure of the total palivizumab glycan pool compared to the intact glycoprotein/glycopeptide approach.					
35572846	2	60	from	mortality	369:377	arg1	infants					382:388	infants	382:388	infants	382:388	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	4	61	theme	therapeutic	592:602	arg1	mAbs					604:607	therapeutic mAbs	592:607	therapeutic mAbs	592:607	The virus neutralization efficacy often depends on the nature and abundance of the glycoforms in therapeutic mAbs.					
35572846	5	62	theme	thorough	623:630	arg1	glycosylation					676:688	glycosylation	676:688	especially glycosylation	665:688	Therefore, a thorough estimation of their PTM profile, especially glycosylation, is relevant.					
35572846	5	62	theme	thorough	623:630	arg1	relevant					694:701	relevant	694:701	relevant	694:701	Therefore, a thorough estimation of their PTM profile, especially glycosylation, is relevant.					
35572846	5	62	theme	thorough	623:630	arg1	estimation					632:641	a thorough estimation	621:641	a thorough estimation of their PTM profile	621:662	Therefore, a thorough estimation of their PTM profile, especially glycosylation, is relevant.					
35572846	9	63	theme	heavy	1174:1178	arg1	chain					1180:1184	the heavy chain	1170:1184	the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans	1170:1303	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	2	64	theme	RNA	303:305	arg1	virus					307:311	another highly contagious negative-sense RNA virus	262:311	another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants	262:388	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	1	65	theme	ongoing	198:204	arg1	pandemic					215:222	the ongoing COVID-19 pandemic	194:222	the ongoing COVID-19 pandemic	194:222	Viral infections are progressively becoming a global health burden, as witnessed in the ongoing COVID-19 pandemic.					
35572846	2	66	theme	tract	343:347	arg1	infections					349:358	lower respiratory tract infections	325:358	lower respiratory tract infections	325:358	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	9	67	theme	minor	1376:1380	arg1	Man5					1396:1399	Man5	1396:1399	Man5 (3.96%)	1396:1407	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	9	67	theme	minor	1376:1380	arg1	G1F					1382:1384	minor G1F' (5.81%)	1376:1393	minor G1F' (5.81%)	1376:1393	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	9	67	theme	minor	1376:1380	arg1	%					1392:1392	5.81%	1388:1392	5.81%	1388:1392	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	13	68	theme	due	1985:1987	arg1	assessment					1974:1983	a more comprehensive glycoprofile assessment	1940:1983	a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition	1940:2118	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	12	69	theme	correct	1779:1785	arg1	measure					1787:1793	a correct measure	1777:1793	a correct measure of the total palivizumab glycan pool	1777:1830	The findings of the study highlight the efficiency of the release glycan method in providing a correct measure of the total palivizumab glycan pool compared to the intact glycoprotein/glycopeptide approach.					
35572846	6	70	theme	palivizumab	765:775	arg1	analysis					753:760	the intact and released glycan analysis	722:760	the intact and released glycan analysis of palivizumab (Synagis®)	722:786	Here, we describe the intact and released glycan analysis of palivizumab (Synagis®) using HILIC chromatography and mass spectrometry.					
35572846	2	71	theme	lower	325:329	arg1	infections					349:358	lower respiratory tract infections	325:358	lower respiratory tract infections	325:358	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	16	72	theme	supplementary	2570:2582	arg1	material					2584:2591	supplementary material	2570:2591	supplementary material available at 10.1007/s42485-022-00086-1	2570:2631	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00086-1.					
35572846	8	73	gly	glycopeptide	1119:1130	arg2	glycopeptide					1119:1130	a glycopeptide	1117:1130	a glycopeptide	1117:1130	The mapping of the peptide and glycopeptides using LC-ESI-MS led to the detection of associated PTMs and the direct identification of a glycopeptide, GlcNAc3Man2.					
35572846	11	74	theme	previous	1666:1673	arg1	studies					1675:1681	previous studies	1666:1681	previous studies	1666:1681	The estimated monosaccharide composition was consistent with previous studies.					
35572846	13	75	theme	labels	2024:2029	arg1	use					2005:2007	the parallel use	1992:2007	the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition	1992:2118	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	12	76	theme	total	1802:1806	arg1	pool					1827:1830	the total palivizumab glycan pool	1798:1830	the total palivizumab glycan pool	1798:1830	The findings of the study highlight the efficiency of the release glycan method in providing a correct measure of the total palivizumab glycan pool compared to the intact glycoprotein/glycopeptide approach.					
35572846	16	77	theme	available	2593:2601	arg1	material					2584:2591	supplementary material	2570:2591	supplementary material available at 10.1007/s42485-022-00086-1	2570:2631	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00086-1.					
35572846	12	78	theme	glycan	1820:1825	arg1	pool					1827:1830	the total palivizumab glycan pool	1798:1830	the total palivizumab glycan pool	1798:1830	The findings of the study highlight the efficiency of the release glycan method in providing a correct measure of the total palivizumab glycan pool compared to the intact glycoprotein/glycopeptide approach.					
35572846	9	79	theme	glycan	1209:1214	arg1	analysis					1216:1223	palivizumab.Release glycan analysis	1189:1223	palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans	1189:1303	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	9	80	theme	typical	1254:1260	arg1	profile					1269:1275	a typical glycan profile	1252:1275	a typical glycan profile consisting of major glycans	1252:1303	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	2	81	theme	contagious	277:286	arg1	virus					307:311	another highly contagious negative-sense RNA virus	262:311	another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants	262:388	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	11	82	with	consistent	1650:1659	arg1	studies					1675:1681	previous studies	1666:1681	previous studies	1666:1681	The estimated monosaccharide composition was consistent with previous studies.					
35572846	1	83	theme	global	156:161	arg1	burden					170:175	a global health burden	154:175	a global health burden	154:175	Viral infections are progressively becoming a global health burden, as witnessed in the ongoing COVID-19 pandemic.					
35572846	8	84	gly	glycopeptides	1014:1026	arg2	glycopeptides					1014:1026	glycopeptides	1014:1026	glycopeptides using LC-ESI-MS	1014:1042	The mapping of the peptide and glycopeptides using LC-ESI-MS led to the detection of associated PTMs and the direct identification of a glycopeptide, GlcNAc3Man2.					
35572846	11	85	theme	estimated	1609:1617	arg1	composition					1634:1644	The estimated monosaccharide composition	1605:1644	The estimated monosaccharide composition	1605:1644	The estimated monosaccharide composition was consistent with previous studies.					
35572846	11	85	theme	estimated	1609:1617	arg1	consistent					1650:1659	consistent	1650:1659	consistent	1650:1659	The estimated monosaccharide composition was consistent with previous studies.					
35572846	0	86	theme	mass	91:94	arg1	spectrometry					96:107	mass spectrometry	91:107	mass spectrometry	91:107	Post-translational modifications and glycoprofiling of palivizumab by UHPLC-RPLC/HILIC and mass spectrometry.					
35572846	13	87	theme	MS	1916:1917	arg1	combinations					1919:1930	The UHPLC-RPLC/HILIC and MS combinations	1891:1930	combinations	1919:1930	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	4	88	theme	neutralization	505:518	arg1	efficacy					520:527	The virus neutralization efficacy	495:527	The virus neutralization efficacy	495:527	The virus neutralization efficacy often depends on the nature and abundance of the glycoforms in therapeutic mAbs.					
35572846	14	89	theme	mAbs	2212:2215	arg1	testing					2165:2171	quick quality testing	2151:2171	quick quality testing	2151:2171	This approach is suitable for quick quality testing and market surveillance of therapeutic mAbs.					
35572846	14	89	theme	mAbs	2212:2215	arg1	surveillance					2184:2195	market surveillance	2177:2195	market surveillance	2177:2195	This approach is suitable for quick quality testing and market surveillance of therapeutic mAbs.					
35572846	7	90	theme	glycosylated	957:968	arg1	palivizumab					970:980	intact glycosylated palivizumab	950:980	intact glycosylated palivizumab	950:980	We detected five glycoforms (Man5/G0FB, G0F/G1F, G1F/G1F, G0FB/G0FB, and G2F/G2F) in deconvoluted MS spectra of intact glycosylated palivizumab.					
35572846	16	91	theme	Supplementary	2516:2528	arg1	Information					2530:2540	Supplementary Information	2516:2540	Supplementary Information The online version	2516:2559	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00086-1.					
35572846	13	92	theme	fluorescent	2012:2022	arg1	labels					2024:2029	fluorescent labels	2012:2029	fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition	2012:2118	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	15	93	theme	palivizumab	2357:2367	arg1	variants					2345:2352	next-generation variants	2329:2352	next-generation variants	2329:2352	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	15	93	theme	palivizumab	2357:2367	arg1	MEDI8897					2378:2385	MEDI8897	2378:2385	MEDI8897	2378:2385	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	15	94	theme	drug	2473:2476	arg1	characterization					2478:2493	drug characterization	2473:2493	drug characterization	2473:2493	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	9	95	theme	G0FB	1413:1416	arg1	forms					1426:1430	G0FB (2.26%) forms	1413:1430	G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'	1413:1480	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	14	96	theme	market	2177:2182	arg1	surveillance					2184:2195	market surveillance	2177:2195	market surveillance	2177:2195	This approach is suitable for quick quality testing and market surveillance of therapeutic mAbs.					
35572846	15	97	theme	variants	2345:2352	arg1	need					2245:2248	a well-perceived need	2228:2248	a well-perceived need for cost-effective immunoprophylaxis	2228:2285	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	15	97	theme	variants	2345:2352	arg1	development					2314:2324	the ongoing fast-paced development	2291:2324	the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897	2291:2385	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	7	98	theme	MS	936:937	arg1	spectra					939:945	deconvoluted MS spectra	923:945	deconvoluted MS spectra of intact glycosylated palivizumab	923:980	We detected five glycoforms (Man5/G0FB, G0F/G1F, G1F/G1F, G0FB/G0FB, and G2F/G2F) in deconvoluted MS spectra of intact glycosylated palivizumab.					
35572846	13	99	theme	N-glycan	2066:2073	arg1	release					2055:2061	the release	2051:2061	the release of N-glycan, sialic acid, and monosaccharide composition	2051:2118	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	13	100	theme	comprehensive	1947:1959	arg1	assessment					1974:1983	a more comprehensive glycoprofile assessment	1940:1983	a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition	1940:2118	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	1	101	theme	Viral	110:114	arg1	infections					116:125	Viral infections	110:125	Viral infections	110:125	Viral infections are progressively becoming a global health burden, as witnessed in the ongoing COVID-19 pandemic.					
35572846	14	102	theme	quick	2151:2155	arg1	testing					2165:2171	quick quality testing	2151:2171	quick quality testing	2151:2171	This approach is suitable for quick quality testing and market surveillance of therapeutic mAbs.					
35572846	15	103	theme	fast-paced	2303:2312	arg1	development					2314:2324	the ongoing fast-paced development	2291:2324	the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897	2291:2385	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	4	104	from	abundance	561:569	arg1	mAbs					604:607	therapeutic mAbs	592:607	therapeutic mAbs	592:607	The virus neutralization efficacy often depends on the nature and abundance of the glycoforms in therapeutic mAbs.					
35572846	15	105	theme	critical	2428:2435	arg1	parameter					2437:2445	a critical parameter	2426:2445	a critical parameter that needs monitoring for drug characterization and quality control	2426:2513	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	15	105	theme	critical	2428:2435	arg1	glycosylation					2409:2421	glycosylation	2409:2421	glycosylation	2409:2421	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	7	106	gly	glycosylated	957:968	arg1	palivizumab					970:980	intact glycosylated palivizumab	950:980	intact glycosylated palivizumab	950:980	We detected five glycoforms (Man5/G0FB, G0F/G1F, G1F/G1F, G0FB/G0FB, and G2F/G2F) in deconvoluted MS spectra of intact glycosylated palivizumab.					
35572846	4	107	from	nature	550:555	arg1	mAbs					604:607	therapeutic mAbs	592:607	therapeutic mAbs	592:607	The virus neutralization efficacy often depends on the nature and abundance of the glycoforms in therapeutic mAbs.					
35572846	12	108	theme	glycan	1750:1755	arg1	method					1757:1762	the release glycan method	1738:1762	the release glycan method	1738:1762	The findings of the study highlight the efficiency of the release glycan method in providing a correct measure of the total palivizumab glycan pool compared to the intact glycoprotein/glycopeptide approach.					
35572846	9	109	theme	superior	1441:1448	arg1	resolution					1450:1459	the superior resolution	1437:1459	the superior resolution of isomeric G1F/G1F'	1437:1480	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	3	110	theme	monoclonal	436:445	arg1	mAb					457:459	mAb	457:459	mAb	457:459	Palivizumab (Synagis®) is the only humanized monoclonal antibody (mAb) approved by the FDA against RSV.					
35572846	3	110	theme	monoclonal	436:445	arg1	antibody					447:454	the only humanized monoclonal antibody	417:454	the only humanized monoclonal antibody (mAb) approved by the FDA against RSV	417:492	Palivizumab (Synagis®) is the only humanized monoclonal antibody (mAb) approved by the FDA against RSV.					
35572846	3	110	theme	monoclonal	436:445	arg1	Palivizumab					391:401	Palivizumab	391:401	Palivizumab (Synagis®)	391:412	Palivizumab (Synagis®) is the only humanized monoclonal antibody (mAb) approved by the FDA against RSV.					
35572846	13	111	theme	UHPLC-RPLC/HILIC	1895:1910	arg1	combinations					1919:1930	The UHPLC-RPLC/HILIC and MS combinations	1891:1930	combinations	1919:1930	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	10	112	theme	first	1504:1508	arg1	evidence					1523:1530	the first experimental evidence	1500:1530	the first experimental evidence of Neu5Gc in the commercial palivizumab formulation using DMB labelling	1500:1602	Next, we provide the first experimental evidence of Neu5Gc in the commercial palivizumab formulation using DMB labelling.					
35572846	15	113	theme	ongoing	2295:2301	arg1	development					2314:2324	the ongoing fast-paced development	2291:2324	the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897	2291:2385	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	9	114	with	G1F	1382:1384	arg1	resolution					1450:1459	the superior resolution	1437:1459	the superior resolution of isomeric G1F/G1F'	1437:1480	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	2	115	theme	Syncytial	237:245	arg1	RSV					254:256	RSV	254:256	RSV	254:256	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	2	115	theme	Syncytial	237:245	arg1	Virus					247:251	Respiratory Syncytial Virus	225:251	Respiratory Syncytial Virus (RSV)	225:257	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	12	116	theme	method	1757:1762	arg1	efficiency					1724:1733	the efficiency	1720:1733	the efficiency of the release glycan method	1720:1762	The findings of the study highlight the efficiency of the release glycan method in providing a correct measure of the total palivizumab glycan pool compared to the intact glycoprotein/glycopeptide approach.					
35572846	7	117	dep	glycoforms	855:864	arg1	G0F/G1F					878:884	G0F/G1F	878:884	G0F/G1F	878:884	We detected five glycoforms (Man5/G0FB, G0F/G1F, G1F/G1F, G0FB/G0FB, and G2F/G2F) in deconvoluted MS spectra of intact glycosylated palivizumab.					
35572846	7	117	dep	glycoforms	855:864	arg1	glycoforms					855:864	five glycoforms	850:864	five glycoforms (Man5/G0FB, G0F/G1F, G1F/G1F, G0FB/G0FB, and G2F/G2F)	850:918	We detected five glycoforms (Man5/G0FB, G0F/G1F, G1F/G1F, G0FB/G0FB, and G2F/G2F) in deconvoluted MS spectra of intact glycosylated palivizumab.					
35572846	7	117	dep	glycoforms	855:864	arg1	G0FB/G0FB					896:904	G0FB/G0FB	896:904	G0FB/G0FB	896:904	We detected five glycoforms (Man5/G0FB, G0F/G1F, G1F/G1F, G0FB/G0FB, and G2F/G2F) in deconvoluted MS spectra of intact glycosylated palivizumab.					
35572846	7	117	dep	glycoforms	855:864	arg1	G2F/G2F					911:917	G2F/G2F	911:917	G2F/G2F	911:917	We detected five glycoforms (Man5/G0FB, G0F/G1F, G1F/G1F, G0FB/G0FB, and G2F/G2F) in deconvoluted MS spectra of intact glycosylated palivizumab.					
35572846	2	118	theme	high	364:367	arg1	mortality					369:377	high mortality	364:377	high mortality in infants	364:388	Respiratory Syncytial Virus (RSV) is another highly contagious negative-sense RNA virus that causes lower respiratory tract infections and high mortality in infants.					
35572846	13	119	theme	monosaccharide	2093:2106	arg1	composition					2108:2118	monosaccharide composition	2093:2118	monosaccharide composition	2093:2118	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
35572846	8	120	theme	PTMs	1079:1082	arg1	detection					1055:1063	the detection	1051:1063	the detection of associated PTMs	1051:1082	The mapping of the peptide and glycopeptides using LC-ESI-MS led to the detection of associated PTMs and the direct identification of a glycopeptide, GlcNAc3Man2.					
35572846	8	120	theme	PTMs	1079:1082	arg1	GlcNAc3Man2					1133:1143	GlcNAc3Man2	1133:1143	GlcNAc3Man2	1133:1143	The mapping of the peptide and glycopeptides using LC-ESI-MS led to the detection of associated PTMs and the direct identification of a glycopeptide, GlcNAc3Man2.					
35572846	8	120	theme	PTMs	1079:1082	arg1	identification					1099:1112	the direct identification	1088:1112	the direct identification of a glycopeptide	1088:1130	The mapping of the peptide and glycopeptides using LC-ESI-MS led to the detection of associated PTMs and the direct identification of a glycopeptide, GlcNAc3Man2.					
35572846	12	121	gly	glycoprotein/glycopeptide	1855:1879	arg2	glycoprotein/glycopeptide					1855:1879	the intact glycoprotein/glycopeptide approach	1844:1888	the intact glycoprotein/glycopeptide approach	1844:1888	The findings of the study highlight the efficiency of the release glycan method in providing a correct measure of the total palivizumab glycan pool compared to the intact glycoprotein/glycopeptide approach.					
35572846	10	122	theme	Neu5Gc	1535:1540	arg1	evidence					1523:1530	the first experimental evidence	1500:1530	the first experimental evidence of Neu5Gc in the commercial palivizumab formulation using DMB labelling	1500:1602	Next, we provide the first experimental evidence of Neu5Gc in the commercial palivizumab formulation using DMB labelling.					
35572846	9	123	attach	derived	1157:1163	arg2	G0F					1306:1308	G0F	1306:1308	G0F	1306:1308	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	9	123	attach	derived	1157:1163	arg2	G1F					1320:1322	G1F	1320:1322	G1F	1320:1322	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	9	123	attach	derived	1157:1163	arg2	G2F					1334:1336	G2F	1334:1336	G2F	1334:1336	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	9	123	attach	derived	1157:1163	arg1	chain					1180:1184	the heavy chain	1170:1184	the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans	1170:1303	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	9	123	attach	derived	1157:1163	arg2	EEQYNSTYR					1146:1154	EEQYNSTYR	1146:1154	EEQYNSTYR	1146:1154	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	9	123	attach	derived	1157:1163	arg2	%					1344:1344	17.24%	1339:1344	17.24%	1339:1344	EEQYNSTYR, derived from the heavy chain of palivizumab.Release glycan analysis using UHPLC-HILIC revealed a typical glycan profile consisting of major glycans, G0F (33.94%), G1F (35.50%), G2F (17.24%) also reported previously and minor G1F' (5.81%), Man5 (3.96%) and G0FB (2.26%) forms with the superior resolution of isomeric G1F/G1F'.					
35572846	6	124	theme	mass	819:822	arg1	spectrometry					824:835	mass spectrometry	819:835	mass spectrometry	819:835	Here, we describe the intact and released glycan analysis of palivizumab (Synagis®) using HILIC chromatography and mass spectrometry.					
35572846	15	125	theme	quality	2499:2505	arg1	control					2507:2513	quality control	2499:2513	quality control	2499:2513	Alongside a well-perceived need for cost-effective immunoprophylaxis and the ongoing fast-paced development of next-generation variants of palivizumab, such as MEDI8897, the study reiterates glycosylation as a critical parameter that needs monitoring for drug characterization and quality control.					
35572846	13	126	theme	sialic	2076:2081	arg1	acid					2083:2086	sialic acid	2076:2086	sialic acid	2076:2086	The UHPLC-RPLC/HILIC and MS combinations provide a more comprehensive glycoprofile assessment due to the parallel use of fluorescent labels for the analysis of the release of N-glycan, sialic acid, and monosaccharide composition.					
36103798	2	0	theme	fermentation	476:487	arg1	method					489:494	the existing chemical or microbial fermentation method	441:494	the existing chemical or microbial fermentation method	441:494	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	1	1	theme	market	339:344	arg1	capacity					346:353	NMN market capacity	335:353	NMN market capacity in the fields of food and health care	335:391	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	1	2	from	growth	325:330	arg1	fields					362:367	the fields	358:367	the fields of food and health care	358:391	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	1	3	from	precursor	193:201	arg1	synthesis					210:218	the synthesis	206:218	the synthesis of nicotinamide adenine dinucleotide (NAD+)	206:262	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	6	4	theme	environmental	1233:1245	arg1	technology					1277:1286	an environmental friendliness biomanufacturing technology	1230:1286	an environmental friendliness biomanufacturing technology for the production of NMN	1230:1312	This in vitro enzymatic platform provides an environmental friendliness biomanufacturing technology for the production of NMN, showing a highly promising alternative approach for NMN production.					
36103798	2	5	theme	microbial	466:474	arg1	method					489:494	the existing chemical or microbial fermentation method	441:494	the existing chemical or microbial fermentation method	441:494	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	1	6	theme	capacity	346:353	arg1	growth					325:330	the rapid growth	315:330	the rapid growth of NMN market capacity in the fields of food and health care	315:391	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	6	7	dep	in	1193:1194	arg1	vitro					1196:1200	vitro	1196:1200	vitro	1196:1200	This in vitro enzymatic platform provides an environmental friendliness biomanufacturing technology for the production of NMN, showing a highly promising alternative approach for NMN production.					
36103798	2	8	with	strategy	561:568	arg1	cost					579:582	low cost	575:582	low cost	575:582	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	2	8	with	strategy	561:568	arg1	pollution					589:597	low pollution	585:597	low pollution	585:597	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	2	8	with	strategy	561:568	arg1	yield					609:613	high yield	604:613	high yield	604:613	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	6	9	theme	NMN	1367:1369	arg1	production					1371:1380	NMN production	1367:1380	NMN production	1367:1380	This in vitro enzymatic platform provides an environmental friendliness biomanufacturing technology for the production of NMN, showing a highly promising alternative approach for NMN production.					
36103798	2	10	theme	urgent	509:514	arg1	need					516:519	an urgent need	506:519	an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield	506:613	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	4	11	theme	multi-enzyme	796:807	arg1	reaction					817:824	This multi-enzyme cascade reaction	791:824	This multi-enzyme cascade reaction	791:824	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	2	12	theme	low	575:577	arg1	cost					579:582	low cost	575:582	low cost	575:582	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	1	13	theme	multiple	276:283	arg1	benefits					292:299	multiple health benefits	276:299	multiple health benefits	276:299	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	4	14	theme	inorganic	884:892	arg1	phosphate					894:902	inorganic phosphate	884:902	inorganic phosphate concentration	884:916	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	3	15	theme	cascade	683:689	arg1	biocatalysis					691:702	an artificial in vitro multi-enzyme cascade biocatalysis	647:702	an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot	647:788	In this study, we demonstrated an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot.					
36103798	3	16	theme	multi-enzyme	670:681	arg1	biocatalysis					691:702	an artificial in vitro multi-enzyme cascade biocatalysis	647:702	an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot	647:788	In this study, we demonstrated an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot.					
36103798	4	17	theme	pH	852:853	arg1	value					855:859	pH value	852:859	pH value	852:859	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	1	18	theme	health	285:290	arg1	benefits					292:299	multiple health benefits	276:299	multiple health benefits	276:299	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	0	19	from	biomanufacturing	52:67	arg1	nicotinamide					116:127	nicotinamide	116:127	nicotinamide	116:127	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	0	19	from	biomanufacturing	52:67	arg1	starch					105:110	starch	105:110	starch	105:110	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	0	19	from	biomanufacturing	52:67	arg1	one-pot					132:138	one-pot	132:138	one-pot	132:138	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	2	20	theme	high	604:607	arg1	yield					609:613	high yield	604:613	high yield	604:613	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	5	21	theme	initial	1150:1156	arg1	concentration					1162:1174	the initial Nam concentration	1146:1174	the initial Nam concentration of 9.21 mM	1146:1185	Under optimized conditions, a high molar yield of 87.8% for NMN was achieved using 3.2 mM Nam as substrate, and a molar yield of 55.37% for NMN was also achieved under the initial Nam concentration of 9.21 mM.					
36103798	6	22	theme	enzymatic	1202:1210	arg1	platform					1212:1219	This in vitro enzymatic platform	1188:1219	This in vitro enzymatic platform	1188:1219	This in vitro enzymatic platform provides an environmental friendliness biomanufacturing technology for the production of NMN, showing a highly promising alternative approach for NMN production.					
36103798	1	23	theme	important	183:191	arg1	mononucleotide					156:169	β-Nicotinamide mononucleotide	141:169	β-Nicotinamide mononucleotide (NMN)	141:175	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	1	23	theme	important	183:191	arg1	precursor					193:201	an important precursor	180:201	an important precursor	180:201	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	3	24	theme	NMN	775:777	arg1	synthesis					762:770	the synthesis	758:770	the synthesis of NMN in one-pot	758:788	In this study, we demonstrated an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot.					
36103798	4	25	theme	concentration	963:975	arg1	terms					843:847	terms	843:847	terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration	843:975	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	0	26	theme	artificial	3:12	arg1	cascade					27:33	An artificial multi-enzyme cascade	0:33	An artificial multi-enzyme cascade	0:33	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	1	27	theme	food	372:375	arg1	fields					362:367	the fields	358:367	the fields of food and health care	358:391	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	4	28	theme	cascade	809:815	arg1	reaction					817:824	This multi-enzyme cascade reaction	791:824	This multi-enzyme cascade reaction	791:824	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	4	29	theme	composition	926:936	arg1	terms					843:847	terms	843:847	terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration	843:975	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	4	30	theme	phosphate	894:902	arg1	concentration					904:916	inorganic phosphate concentration	884:916	inorganic phosphate concentration	884:916	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	6	31	theme	alternative	1342:1352	arg1	approach					1354:1361	a highly promising alternative approach	1323:1361	a highly promising alternative approach for NMN production	1323:1380	This in vitro enzymatic platform provides an environmental friendliness biomanufacturing technology for the production of NMN, showing a highly promising alternative approach for NMN production.					
36103798	2	32	theme	production	427:436	arg1	drawbacks					410:418	the drawbacks	406:418	the drawbacks of NMN production	406:436	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	2	33	theme	low	585:587	arg1	pollution					589:597	low pollution	585:597	low pollution	585:597	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	5	34	theme	high	1008:1011	arg1	yield					1019:1023	a high molar yield	1006:1023	a high molar yield of 87.8% for NMN	1006:1040	Under optimized conditions, a high molar yield of 87.8% for NMN was achieved using 3.2 mM Nam as substrate, and a molar yield of 55.37% for NMN was also achieved under the initial Nam concentration of 9.21 mM.					
36103798	5	35	theme	%	1032:1032	arg1	yield					1019:1023	a high molar yield	1006:1023	a high molar yield of 87.8% for NMN	1006:1040	Under optimized conditions, a high molar yield of 87.8% for NMN was achieved using 3.2 mM Nam as substrate, and a molar yield of 55.37% for NMN was also achieved under the initial Nam concentration of 9.21 mM.					
36103798	2	36	theme	NMN	423:425	arg1	production					427:436	NMN production	423:436	NMN production	423:436	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	4	37	theme	buffer	862:867	arg1	concentration					869:881	buffer concentration	862:881	buffer concentration	862:881	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	3	38	theme	artificial	650:659	arg1	biocatalysis					691:702	an artificial in vitro multi-enzyme cascade biocatalysis	647:702	an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot	647:788	In this study, we demonstrated an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot.					
36103798	0	39	theme	multi-enzyme	14:25	arg1	cascade					27:33	An artificial multi-enzyme cascade	0:33	An artificial multi-enzyme cascade	0:33	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	5	40	theme	%	1112:1112	arg1	yield					1098:1102	a molar yield	1090:1102	a molar yield of 55.37% for NMN	1090:1120	Under optimized conditions, a high molar yield of 87.8% for NMN was achieved using 3.2 mM Nam as substrate, and a molar yield of 55.37% for NMN was also achieved under the initial Nam concentration of 9.21 mM.					
36103798	3	41	from	NMN	775:777	arg1	one-pot					782:788	one-pot	782:788	one-pot	782:788	In this study, we demonstrated an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot.					
36103798	0	42	from	starch	105:110	arg1	biomanufacturing					52:67	biomanufacturing	52:67	biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot	52:138	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	0	42	from	starch	105:110	arg1	mononucleotide					85:98	nicotinamide mononucleotide	72:98	nicotinamide mononucleotide from starch and nicotinamide in one-pot	72:138	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	5	43	theme	9.21 mM	1179:1185	arg1	concentration					1162:1174	the initial Nam concentration	1146:1174	the initial Nam concentration of 9.21 mM	1146:1185	Under optimized conditions, a high molar yield of 87.8% for NMN was achieved using 3.2 mM Nam as substrate, and a molar yield of 55.37% for NMN was also achieved under the initial Nam concentration of 9.21 mM.					
36103798	1	44	from	capacity	346:353	arg1	fields					362:367	the fields	358:367	the fields of food and health care	358:391	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	3	45	dep	in	661:662	arg1	vitro					664:668	vitro	664:668	vitro	664:668	In this study, we demonstrated an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot.					
36103798	6	46	theme	biomanufacturing	1260:1275	arg1	technology					1277:1286	an environmental friendliness biomanufacturing technology	1230:1286	an environmental friendliness biomanufacturing technology for the production of NMN	1230:1312	This in vitro enzymatic platform provides an environmental friendliness biomanufacturing technology for the production of NMN, showing a highly promising alternative approach for NMN production.					
36103798	1	47	theme	health	381:386	arg1	care					388:391	health care	381:391	health care	381:391	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	5	48	theme	optimized	984:992	arg1	conditions					994:1003	optimized conditions	984:1003	optimized conditions	984:1003	Under optimized conditions, a high molar yield of 87.8% for NMN was achieved using 3.2 mM Nam as substrate, and a molar yield of 55.37% for NMN was also achieved under the initial Nam concentration of 9.21 mM.					
36103798	1	49	theme	nicotinamide	223:234	arg1	NAD+					258:261	NAD+	258:261	NAD+	258:261	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	1	49	theme	nicotinamide	223:234	arg1	dinucleotide					244:255	nicotinamide adenine dinucleotide	223:255	nicotinamide adenine dinucleotide (NAD+)	223:262	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	2	50	theme	chemical	454:461	arg1	method					489:494	the existing chemical or microbial fermentation method	441:494	the existing chemical or microbial fermentation method	441:494	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	3	51	from	one-pot	782:788	arg1	synthesis					762:770	the synthesis	758:770	the synthesis of NMN in one-pot	758:788	In this study, we demonstrated an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot.					
36103798	0	52	from	mononucleotide	85:98	arg1	one-pot					132:138	one-pot	132:138	one-pot	132:138	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	5	53	theme	3.2 mM	1061:1066	arg1	Nam					1068:1070	3.2 mM Nam	1061:1070	3.2 mM Nam	1061:1070	Under optimized conditions, a high molar yield of 87.8% for NMN was achieved using 3.2 mM Nam as substrate, and a molar yield of 55.37% for NMN was also achieved under the initial Nam concentration of 9.21 mM.					
36103798	1	54	theme	adenine	236:242	arg1	NAD+					258:261	NAD+	258:261	NAD+	258:261	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	1	54	theme	adenine	236:242	arg1	dinucleotide					244:255	nicotinamide adenine dinucleotide	223:255	nicotinamide adenine dinucleotide (NAD+)	223:262	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	2	55	theme	existing	445:452	arg1	method					489:494	the existing chemical or microbial fermentation method	441:494	the existing chemical or microbial fermentation method	441:494	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	1	56	theme	rapid	319:323	arg1	growth					325:330	the rapid growth	315:330	the rapid growth of NMN market capacity in the fields of food and health care	315:391	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	6	57	theme	NMN	1310:1312	arg1	production					1296:1305	the production	1292:1305	the production of NMN	1292:1312	This in vitro enzymatic platform provides an environmental friendliness biomanufacturing technology for the production of NMN, showing a highly promising alternative approach for NMN production.					
36103798	4	58	theme	enzyme	919:924	arg1	composition					926:936	enzyme composition	919:936	enzyme composition	919:936	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	4	59	theme	value	855:859	arg1	terms					843:847	terms	843:847	terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration	843:975	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	4	60	theme	concentration	869:881	arg1	terms					843:847	terms	843:847	terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration	843:975	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	5	61	theme	molar	1092:1096	arg1	yield					1098:1102	a molar yield	1090:1102	a molar yield of 55.37% for NMN	1090:1120	Under optimized conditions, a high molar yield of 87.8% for NMN was achieved using 3.2 mM Nam as substrate, and a molar yield of 55.37% for NMN was also achieved under the initial Nam concentration of 9.21 mM.					
36103798	2	62	theme	production	550:559	arg1	strategy					561:568	a prospective NMN production strategy	532:568	a prospective NMN production strategy with low cost, low pollution, and high yield	532:613	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	0	63	from	one-pot	132:138	arg1	biomanufacturing					52:67	biomanufacturing	52:67	biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot	52:138	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	3	64	from	synthesis	762:770	arg1	one-pot					782:788	one-pot	782:788	one-pot	782:788	In this study, we demonstrated an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot.					
36103798	1	65	theme	β-Nicotinamide	141:154	arg1	mononucleotide					156:169	β-Nicotinamide mononucleotide	141:169	β-Nicotinamide mononucleotide (NMN)	141:175	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	1	65	theme	β-Nicotinamide	141:154	arg1	precursor					193:201	an important precursor	180:201	an important precursor	180:201	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	1	65	theme	β-Nicotinamide	141:154	arg1	NMN					172:174	NMN	172:174	NMN	172:174	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	2	66	theme	NMN	546:548	arg1	strategy					561:568	a prospective NMN production strategy	532:568	a prospective NMN production strategy with low cost, low pollution, and high yield	532:613	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	5	67	theme	molar	1013:1017	arg1	yield					1019:1023	a high molar yield	1006:1023	a high molar yield of 87.8% for NMN	1006:1040	Under optimized conditions, a high molar yield of 87.8% for NMN was achieved using 3.2 mM Nam as substrate, and a molar yield of 55.37% for NMN was also achieved under the initial Nam concentration of 9.21 mM.					
36103798	4	68	theme	phosphoenolpyruvate	943:961	arg1	concentration					963:975	phosphoenolpyruvate concentration	943:975	phosphoenolpyruvate concentration	943:975	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	6	69	theme	friendliness	1247:1258	arg1	technology					1277:1286	an environmental friendliness biomanufacturing technology	1230:1286	an environmental friendliness biomanufacturing technology for the production of NMN	1230:1312	This in vitro enzymatic platform provides an environmental friendliness biomanufacturing technology for the production of NMN, showing a highly promising alternative approach for NMN production.					
36103798	1	70	theme	dinucleotide	244:255	arg1	synthesis					210:218	the synthesis	206:218	the synthesis of nicotinamide adenine dinucleotide (NAD+)	206:262	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	2	71	theme	prospective	534:544	arg1	strategy					561:568	a prospective NMN production strategy	532:568	a prospective NMN production strategy with low cost, low pollution, and high yield	532:613	To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield.					
36103798	0	72	from	nicotinamide	116:127	arg1	biomanufacturing					52:67	biomanufacturing	52:67	biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot	52:138	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	0	72	from	nicotinamide	116:127	arg1	mononucleotide					85:98	nicotinamide mononucleotide	72:98	nicotinamide mononucleotide from starch and nicotinamide in one-pot	72:138	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	0	73	theme	mononucleotide	85:98	arg1	biomanufacturing					52:67	biomanufacturing	52:67	biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot	52:138	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	6	74	theme	promising	1332:1340	arg1	approach					1354:1361	a highly promising alternative approach	1323:1361	a highly promising alternative approach for NMN production	1323:1380	This in vitro enzymatic platform provides an environmental friendliness biomanufacturing technology for the production of NMN, showing a highly promising alternative approach for NMN production.					
36103798	1	75	theme	care	388:391	arg1	fields					362:367	the fields	358:367	the fields of food and health care	358:391	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	0	76	theme	nicotinamide	72:83	arg1	mononucleotide					85:98	nicotinamide mononucleotide	72:98	nicotinamide mononucleotide from starch and nicotinamide in one-pot	72:138	An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot.					
36103798	1	77	theme	NMN	335:337	arg1	capacity					346:353	NMN market capacity	335:353	NMN market capacity in the fields of food and health care	335:391	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	1	78	from	fields	362:367	arg1	growth					325:330	the rapid growth	315:330	the rapid growth of NMN market capacity in the fields of food and health care	315:391	β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care.					
36103798	3	79	theme	in	661:662	arg1	biocatalysis					691:702	an artificial in vitro multi-enzyme cascade biocatalysis	647:702	an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot	647:788	In this study, we demonstrated an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot.					
36103798	4	80	theme	concentration	904:916	arg1	terms					843:847	terms	843:847	terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration	843:975	This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration.					
36103798	6	81	theme	in	1193:1194	arg1	platform					1212:1219	This in vitro enzymatic platform	1188:1219	This in vitro enzymatic platform	1188:1219	This in vitro enzymatic platform provides an environmental friendliness biomanufacturing technology for the production of NMN, showing a highly promising alternative approach for NMN production.					
36103798	5	82	theme	Nam	1158:1160	arg1	concentration					1162:1174	the initial Nam concentration	1146:1174	the initial Nam concentration of 9.21 mM	1146:1185	Under optimized conditions, a high molar yield of 87.8% for NMN was achieved using 3.2 mM Nam as substrate, and a molar yield of 55.37% for NMN was also achieved under the initial Nam concentration of 9.21 mM.					
36080292	0	0	theme	Lactation	65:73	arg1	Stages					55:60	Different Stages	45:60	Different Stages of Lactation	45:73	1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors.					
36080292	8	1	theme	non-secretor	1198:1209	arg1	development					1183:1193	the development	1179:1193	the development of non-secretor and secretor HMO profiles	1179:1235	A temporal difference of approximately 1-2 months between the development of non-secretor and secretor HMO profiles was shown.					
36080292	9	2	theme	long-term	1290:1298	arg1	breastfeeding					1300:1312	long-term breastfeeding	1290:1312	long-term breastfeeding	1290:1312	The results highlighted the importance of long-term breastfeeding, especially among non-secretors.					
36080292	3	3	theme	samples	322:328	arg1	total					292:296	A total	290:296	A total of 72 human breast milk samples	290:328	A total of 72 human breast milk samples were collected from ten Chinese mothers at eight different time points.					
36080292	3	4	theme	Chinese	354:360	arg1	mothers					362:368	ten Chinese mothers	350:368	ten Chinese mothers	350:368	A total of 72 human breast milk samples were collected from ten Chinese mothers at eight different time points.					
36080292	2	5	theme	1H	269:270	arg1	metabolomics					276:287	1H NMR metabolomics	269:287	1H NMR metabolomics	269:287	We studied the composition of human milk samples collected over an 8-month lactation using 1H NMR metabolomics.					
36080292	2	6	theme	NMR	272:274	arg1	metabolomics					276:287	1H NMR metabolomics	269:287	1H NMR metabolomics	269:287	We studied the composition of human milk samples collected over an 8-month lactation using 1H NMR metabolomics.					
36080292	9	7	theme	breastfeeding	1300:1312	arg1	importance					1276:1285	the importance	1272:1285	the importance of long-term breastfeeding	1272:1312	The results highlighted the importance of long-term breastfeeding, especially among non-secretors.					
36080292	7	8	theme	fucose	1048:1053	arg1	concentrations					905:918	The concentrations	901:918	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose	901:1053	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	7	8	theme	fucose	1048:1053	arg1	different					1074:1082	different	1074:1082	different	1074:1082	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	4	9	theme	oligosaccharides	439:454	arg1	concentrations					406:419	The concentrations	402:419	The concentrations of ten human milk oligosaccharides (HMOs), fucose and lactose	402:481	The concentrations of ten human milk oligosaccharides (HMOs), fucose and lactose were quantified.					
36080292	7	10	theme	LNFP	995:998	arg1	concentrations					905:918	The concentrations	901:918	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose	901:1053	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	7	10	theme	LNFP	995:998	arg1	different					1074:1082	different	1074:1082	different	1074:1082	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	3	11	theme	breast	310:315	arg1	samples					322:328	72 human breast milk samples	301:328	72 human breast milk samples	301:328	A total of 72 human breast milk samples were collected from ten Chinese mothers at eight different time points.					
36080292	4	12	theme	milk	434:437	arg1	lactose					475:481	lactose	475:481	lactose	475:481	The concentrations of ten human milk oligosaccharides (HMOs), fucose and lactose were quantified.					
36080292	4	12	theme	milk	434:437	arg1	HMOs					457:460	HMOs	457:460	HMOs	457:460	The concentrations of ten human milk oligosaccharides (HMOs), fucose and lactose were quantified.					
36080292	4	12	theme	milk	434:437	arg1	fucose					464:469	fucose	464:469	fucose	464:469	The concentrations of ten human milk oligosaccharides (HMOs), fucose and lactose were quantified.					
36080292	4	12	theme	milk	434:437	arg1	oligosaccharides					439:454	ten human milk oligosaccharides	424:454	ten human milk oligosaccharides (HMOs)	424:461	The concentrations of ten human milk oligosaccharides (HMOs), fucose and lactose were quantified.					
36080292	7	13	theme	LNFP	987:990	arg1	concentrations					905:918	The concentrations	901:918	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose	901:1053	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	7	13	theme	LNFP	987:990	arg1	different					1074:1082	different	1074:1082	different	1074:1082	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	8	14	theme	profiles	1228:1235	arg1	development					1183:1193	the development	1179:1193	the development of non-secretor and secretor HMO profiles	1179:1235	A temporal difference of approximately 1-2 months between the development of non-secretor and secretor HMO profiles was shown.					
36080292	3	15	theme	time	389:392	arg1	points					394:399	eight different time points	373:399	eight different time points	373:399	A total of 72 human breast milk samples were collected from ten Chinese mothers at eight different time points.					
36080292	3	16	theme	milk	317:320	arg1	samples					322:328	72 human breast milk samples	301:328	72 human breast milk samples	301:328	A total of 72 human breast milk samples were collected from ten Chinese mothers at eight different time points.					
36080292	5	17	theme	lactodifucotetraose	704:722	arg1	levels					635:640	the levels	631:640	the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT)	631:760	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	2	18	theme	8-month	245:251	arg1	lactation					253:261	an 8-month lactation	242:261	an 8-month lactation using 1H NMR metabolomics	242:287	We studied the composition of human milk samples collected over an 8-month lactation using 1H NMR metabolomics.					
36080292	4	19	theme	human	428:432	arg1	lactose					475:481	lactose	475:481	lactose	475:481	The concentrations of ten human milk oligosaccharides (HMOs), fucose and lactose were quantified.					
36080292	4	19	theme	human	428:432	arg1	HMOs					457:460	HMOs	457:460	HMOs	457:460	The concentrations of ten human milk oligosaccharides (HMOs), fucose and lactose were quantified.					
36080292	4	19	theme	human	428:432	arg1	fucose					464:469	fucose	464:469	fucose	464:469	The concentrations of ten human milk oligosaccharides (HMOs), fucose and lactose were quantified.					
36080292	4	19	theme	human	428:432	arg1	oligosaccharides					439:454	ten human milk oligosaccharides	424:454	ten human milk oligosaccharides (HMOs)	424:461	The concentrations of ten human milk oligosaccharides (HMOs), fucose and lactose were quantified.					
36080292	0	20	theme	NMR	3:5	arg1	Metabolomics					7:18	1H NMR Metabolomics	0:18	1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors.	0:108	1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors.					
36080292	7	21	theme	LDFT	981:984	arg1	concentrations					905:918	The concentrations	901:918	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose	901:1053	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	7	21	theme	LDFT	981:984	arg1	different					1074:1082	different	1074:1082	different	1074:1082	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	7	22	theme	3'-SL	955:959	arg1	concentrations					905:918	The concentrations	901:918	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose	901:1053	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	7	22	theme	3'-SL	955:959	arg1	different					1074:1082	different	1074:1082	different	1074:1082	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	1	23	theme	infant	163:168	arg1	health					170:175	infant health	163:175	infant health	163:175	Human milk is an intricate, bioactive food promoting infant health.					
36080292	0	24	theme	1H	0:1	arg1	Metabolomics					7:18	1H NMR Metabolomics	0:18	1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors.	0:108	1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors.					
36080292	6	25	theme	lactation	890:898	arg1	stage					881:885	the stage	877:885	the stage of lactation	877:898	Acetate, citrate, short/medium-chain fatty acids, glutamine and urea showed a time-dependent trend in relation to the stage of lactation.					
36080292	5	26	theme	Lewis-positive	584:597	arg1	Se-Le+					614:619	Se-Le+	614:619	Se-Le+	614:619	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	26	theme	Lewis-positive	584:597	arg1	Six					500:502	Six	500:502	Six	500:502	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	26	theme	Lewis-positive	584:597	arg1	non-secretors					599:611	Lewis-positive non-secretors	584:611	Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT)	584:760	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	26	theme	Lewis-positive	584:597	arg1	mothers					511:517	the mothers	507:517	the mothers	507:517	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	27	theme	lacto-N-neotetraose	735:753	arg1	levels					635:640	the levels	631:640	the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT)	631:760	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	28	theme	Lewis-positive	538:551	arg1	Se+Le+					564:569	Se+Le+	564:569	Se+Le+	564:569	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	28	theme	Lewis-positive	538:551	arg1	Six					500:502	Six	500:502	Six	500:502	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	28	theme	Lewis-positive	538:551	arg1	secretors					553:561	Lewis-positive secretors	538:561	Lewis-positive secretors (Se+Le+)	538:570	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	28	theme	Lewis-positive	538:551	arg1	mothers					511:517	the mothers	507:517	the mothers	507:517	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	8	29	theme	temporal	1123:1130	arg1	difference					1132:1141	A temporal difference	1121:1141	A temporal difference of approximately 1-2 months between the development of non-secretor and secretor HMO profiles	1121:1235	A temporal difference of approximately 1-2 months between the development of non-secretor and secretor HMO profiles was shown.					
36080292	7	30	theme	LNT	1020:1022	arg1	concentrations					905:918	The concentrations	901:918	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose	901:1053	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	7	30	theme	LNT	1020:1022	arg1	different					1074:1082	different	1074:1082	different	1074:1082	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	5	31	theme	2'-fucosyllactose	645:661	arg1	levels					635:640	the levels	631:640	the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT)	631:760	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	6	32	theme	fatty	800:804	arg1	acids					806:810	short/medium-chain fatty acids	781:810	short/medium-chain fatty acids	781:810	Acetate, citrate, short/medium-chain fatty acids, glutamine and urea showed a time-dependent trend in relation to the stage of lactation.					
36080292	1	33	dep	intricate	127:135	arg1	bioactive					138:146	bioactive	138:146	bioactive	138:146	Human milk is an intricate, bioactive food promoting infant health.					
36080292	7	34	theme	LNnT	1014:1017	arg1	concentrations					905:918	The concentrations	901:918	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose	901:1053	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	7	34	theme	LNnT	1014:1017	arg1	different					1074:1082	different	1074:1082	different	1074:1082	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	0	35	theme	Human	31:35	arg1	Milk					37:40	Chinese Human Milk	23:40	Chinese Human Milk	23:40	1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors.					
36080292	6	36	theme	short/medium-chain	781:798	arg1	acids					806:810	short/medium-chain fatty acids	781:810	short/medium-chain fatty acids	781:810	Acetate, citrate, short/medium-chain fatty acids, glutamine and urea showed a time-dependent trend in relation to the stage of lactation.					
36080292	2	37	theme	milk	214:217	arg1	samples					219:225	human milk samples	208:225	human milk samples collected over an 8-month lactation using 1H NMR metabolomics	208:287	We studied the composition of human milk samples collected over an 8-month lactation using 1H NMR metabolomics.					
36080292	0	38	theme	Chinese	23:29	arg1	Milk					37:40	Chinese Human Milk	23:40	Chinese Human Milk	23:40	1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors.					
36080292	3	39	theme	different	379:387	arg1	points					394:399	eight different time points	373:399	eight different time points	373:399	A total of 72 human breast milk samples were collected from ten Chinese mothers at eight different time points.					
36080292	0	40	from	Stages	55:60	arg1	Metabolomics					7:18	1H NMR Metabolomics	0:18	1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors.	0:108	1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors.					
36080292	2	41	theme	human	208:212	arg1	samples					219:225	human milk samples	208:225	human milk samples collected over an 8-month lactation using 1H NMR metabolomics	208:287	We studied the composition of human milk samples collected over an 8-month lactation using 1H NMR metabolomics.					
36080292	7	42	theme	3-FL	930:933	arg1	concentrations					905:918	The concentrations	901:918	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose	901:1053	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	7	42	theme	3-FL	930:933	arg1	different					1074:1082	different	1074:1082	different	1074:1082	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	7	43	theme	LNFP	1004:1007	arg1	concentrations					905:918	The concentrations	901:918	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose	901:1053	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	7	43	theme	LNFP	1004:1007	arg1	different					1074:1082	different	1074:1082	different	1074:1082	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	3	44	theme	human	304:308	arg1	samples					322:328	72 human breast milk samples	301:328	72 human breast milk samples	301:328	A total of 72 human breast milk samples were collected from ten Chinese mothers at eight different time points.					
36080292	0	45	theme	Milk	37:40	arg1	Metabolomics					7:18	1H NMR Metabolomics	0:18	1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors.	0:108	1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors.					
36080292	6	46	from	trend	856:860	arg1	relation					865:872	relation	865:872	relation to the stage of lactation	865:898	Acetate, citrate, short/medium-chain fatty acids, glutamine and urea showed a time-dependent trend in relation to the stage of lactation.					
36080292	7	47	theme	2'-FL	923:927	arg1	concentrations					905:918	The concentrations	901:918	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose	901:1053	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	7	47	theme	2'-FL	923:927	arg1	different					1074:1082	different	1074:1082	different	1074:1082	The concentrations of 2'-FL, 3-FL (3-fucosyllactose), 3'-SL (3'-sialyllactose), LDFT, LNFP I, LNFP II, LNFP III, LNnT, LNT (lacto-N-tetraose), and fucose were statistically different between secretors and non-secretors.					
36080292	1	48	theme	Human	110:114	arg1	milk					116:119	Human milk	110:119	Human milk	110:119	Human milk is an intricate, bioactive food promoting infant health.					
36080292	1	48	theme	Human	110:114	arg1	food					148:151	an intricate, bioactive food	124:151	an intricate, bioactive food promoting infant health	124:175	Human milk is an intricate, bioactive food promoting infant health.					
36080292	5	49	theme	mothers	511:517	arg1	four					576:579	four	576:579	four	576:579	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	49	theme	mothers	511:517	arg1	secretors					553:561	Lewis-positive secretors	538:561	Lewis-positive secretors (Se+Le+)	538:570	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	49	theme	mothers	511:517	arg1	non-secretors					599:611	Lewis-positive non-secretors	584:611	Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT)	584:760	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	49	theme	mothers	511:517	arg1	Six					500:502	Six	500:502	Six	500:502	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	49	theme	mothers	511:517	arg1	mothers					511:517	the mothers	507:517	the mothers	507:517	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	5	50	theme	lacto-N-fucopentaose	672:691	arg1	levels					635:640	the levels	631:640	the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT)	631:760	Six of the mothers were classified as Lewis-positive secretors (Se+Le+) and four as Lewis-positive non-secretors (Se-Le+) based on the levels of 2'-fucosyllactose (2'-FL), lacto-N-fucopentaose (LNFP) II, lactodifucotetraose (LDFT) and lacto-N-neotetraose (LNnT).					
36080292	8	51	theme	HMO	1224:1226	arg1	profiles					1228:1235	secretor HMO profiles	1215:1235	secretor HMO profiles	1215:1235	A temporal difference of approximately 1-2 months between the development of non-secretor and secretor HMO profiles was shown.					
36080292	8	52	theme	secretor	1215:1222	arg1	profiles					1228:1235	secretor HMO profiles	1215:1235	secretor HMO profiles	1215:1235	A temporal difference of approximately 1-2 months between the development of non-secretor and secretor HMO profiles was shown.					
36080292	8	53	theme	months	1164:1169	arg1	difference					1132:1141	A temporal difference	1121:1141	A temporal difference of approximately 1-2 months between the development of non-secretor and secretor HMO profiles	1121:1235	A temporal difference of approximately 1-2 months between the development of non-secretor and secretor HMO profiles was shown.					
36080292	0	54	theme	Different	45:53	arg1	Stages					55:60	Different Stages	45:60	Different Stages of Lactation	45:73	1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors.					
36080292	6	55	theme	time-dependent	841:854	arg1	trend					856:860	a time-dependent trend	839:860	a time-dependent trend in relation to the stage of lactation	839:898	Acetate, citrate, short/medium-chain fatty acids, glutamine and urea showed a time-dependent trend in relation to the stage of lactation.					
36080292	2	56	theme	samples	219:225	arg1	composition					193:203	the composition	189:203	the composition of human milk samples collected over an 8-month lactation using 1H NMR metabolomics	189:287	We studied the composition of human milk samples collected over an 8-month lactation using 1H NMR metabolomics.					
36080292	1	57	theme	intricate	127:135	arg1	milk					116:119	Human milk	110:119	Human milk	110:119	Human milk is an intricate, bioactive food promoting infant health.					
36080292	1	57	theme	intricate	127:135	arg1	food					148:151	an intricate, bioactive food	124:151	an intricate, bioactive food promoting infant health	124:175	Human milk is an intricate, bioactive food promoting infant health.					
35638590	11	0	from	perspective	1932:1942	arg1	pathogenesis					1951:1962	the pathogenesis	1947:1962	the pathogenesis of lung inflammation injury	1947:1990	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	6	1	theme	lung	893:896	arg1	damage					911:916	lung inflammatory damage	893:916	lung inflammatory damage	893:916	Our data revealed that LPS nebulization combined with a high-calorie diet resulted in significant changes in rats, such as slow weight gain, increased lung index, and aggravated lung inflammatory damage.					
35638590	5	2	theme	high-calorie	641:652	arg1	diet					654:657	a high-calorie diet	639:657	a high-calorie diet	639:657	In this study, specific pathogen-free juvenile rats were placed in a routine environment, and subjected to a high-calorie diet or LPS atomization in isolation as well as combination.					
35638590	11	3	theme	lung	2059:2062	arg1	diseases					2064:2071	inflammatory lung diseases	2046:2071	inflammatory lung diseases	2046:2071	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	11	4	theme	lung	1882:1885	arg1	damage					1900:1905	LPS-induced lung inflammatory damage	1870:1905	LPS-induced lung inflammatory damage	1870:1905	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	11	5	theme	injury	1985:1990	arg1	pathogenesis					1951:1962	the pathogenesis	1947:1962	the pathogenesis of lung inflammation injury	1947:1990	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	6	6	theme	slow	838:841	arg1	gain					850:853	slow weight gain	838:853	slow weight gain	838:853	Our data revealed that LPS nebulization combined with a high-calorie diet resulted in significant changes in rats, such as slow weight gain, increased lung index, and aggravated lung inflammatory damage.					
35638590	8	7	theme	gut	1158:1160	arg1	composition					1173:1183	the gut microbiome composition	1154:1183	the gut microbiome composition	1154:1183	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	4	8	theme	juvenile	517:524	arg1	rats					526:529	juvenile rats	517:529	juvenile rats	517:529	Our previous studies demonstrated that a high-calorie diet and LPS atomization synergistically promoted lung inflammation injury in juvenile rats.					
35638590	5	9	theme	routine	601:607	arg1	environment					609:619	a routine environment	599:619	a routine environment	599:619	In this study, specific pathogen-free juvenile rats were placed in a routine environment, and subjected to a high-calorie diet or LPS atomization in isolation as well as combination.					
35638590	11	10	theme	microbiota-targeting	2013:2032	arg1	therapy					2034:2040	a novel microbiota-targeting therapy	2005:2040	a novel microbiota-targeting therapy for inflammatory lung diseases	2005:2071	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	11	11	theme	inflammatory	2046:2057	arg1	diseases					2064:2071	inflammatory lung diseases	2046:2071	inflammatory lung diseases	2046:2071	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	11	12	theme	gut	1769:1771	arg1	microbiome					1773:1782	the gut microbiome	1765:1782	the gut microbiome	1765:1782	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	0	13	theme	Th17/Treg	90:98	arg1	balance					100:106	Th17/Treg balance	90:106	Th17/Treg balance	90:106	A high-calorie diet aggravates LPS-induced pneumonia by disturbing the gut microbiota and Th17/Treg balance.					
35638590	5	14	theme	LPS	662:664	arg1	atomization					666:676	LPS atomization	662:676	LPS atomization	662:676	In this study, specific pathogen-free juvenile rats were placed in a routine environment, and subjected to a high-calorie diet or LPS atomization in isolation as well as combination.					
35638590	6	15	theme	significant	801:811	arg1	gain					850:853	slow weight gain	838:853	slow weight gain	838:853	Our data revealed that LPS nebulization combined with a high-calorie diet resulted in significant changes in rats, such as slow weight gain, increased lung index, and aggravated lung inflammatory damage.					
35638590	6	15	theme	significant	801:811	arg1	changes					813:819	significant changes	801:819	significant changes	801:819	Our data revealed that LPS nebulization combined with a high-calorie diet resulted in significant changes in rats, such as slow weight gain, increased lung index, and aggravated lung inflammatory damage.					
35638590	8	16	from	production	1305:1314	arg1	rats					1354:1357	rats	1354:1357	rats	1354:1357	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	8	17	theme	microbiota	1274:1283	arg1	abundance					1246:1254	the abundance	1242:1254	the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats	1242:1357	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	10	18	theme	high-calorie	1555:1566	arg1	diet					1568:1571	a high-calorie diet	1553:1571	a high-calorie diet	1553:1571	More critically, the effects of a high-calorie diet were shown to be transmissible among pneumonia rats through cohousing microbiota transplantation.					
35638590	4	19	theme	previous	389:396	arg1	studies					398:404	Our previous studies	385:404	Our previous studies	385:404	Our previous studies demonstrated that a high-calorie diet and LPS atomization synergistically promoted lung inflammation injury in juvenile rats.					
35638590	1	20	theme	inflammatory	161:172	arg1	response					174:181	the inflammatory response	157:181	the inflammatory response to the systemic or local infections in the host	157:229	The intestinal flora plays an important role in the inflammatory response to the systemic or local infections in the host.					
35638590	8	21	theme	microbiome	1162:1171	arg1	composition					1173:1183	the gut microbiome composition	1154:1183	the gut microbiome composition	1154:1183	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	4	22	theme	inflammation	494:505	arg1	injury					507:512	lung inflammation injury	489:512	lung inflammation injury	489:512	Our previous studies demonstrated that a high-calorie diet and LPS atomization synergistically promoted lung inflammation injury in juvenile rats.					
35638590	9	23	theme	diet	1515:1518	arg1	intervention					1484:1495	the intervention	1480:1495	the intervention of a high-calorie diet	1480:1518	Consequently, the levels of SCFAs, especially acetate, propionate, and butyrate, were significantly decreased following the intervention of a high-calorie diet.					
35638590	11	24	theme	novel	2007:2011	arg1	therapy					2034:2040	a novel microbiota-targeting therapy	2005:2040	a novel microbiota-targeting therapy for inflammatory lung diseases	2005:2071	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	0	25	theme	high-calorie	2:13	arg1	diet					15:18	A high-calorie diet	0:18	A high-calorie diet	0:18	A high-calorie diet aggravates LPS-induced pneumonia by disturbing the gut microbiota and Th17/Treg balance.					
35638590	4	26	theme	lung	489:492	arg1	injury					507:512	lung inflammation injury	489:512	lung inflammation injury	489:512	Our previous studies demonstrated that a high-calorie diet and LPS atomization synergistically promoted lung inflammation injury in juvenile rats.					
35638590	8	27	theme	high-calorie	1128:1139	arg1	diet					1141:1144	a high-calorie diet	1126:1144	a high-calorie diet	1126:1144	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	5	28	theme	specific	547:554	arg1	rats					579:582	specific pathogen-free juvenile rats	547:582	specific pathogen-free juvenile rats	547:582	In this study, specific pathogen-free juvenile rats were placed in a routine environment, and subjected to a high-calorie diet or LPS atomization in isolation as well as combination.					
35638590	10	29	theme	microbiota	1643:1652	arg1	transplantation					1654:1668	microbiota transplantation	1643:1668	microbiota transplantation	1643:1668	More critically, the effects of a high-calorie diet were shown to be transmissible among pneumonia rats through cohousing microbiota transplantation.					
35638590	11	30	theme	Th17/Treg	1809:1817	arg1	balance					1824:1830	Th17/Treg cell balance	1809:1830	Th17/Treg cell balance	1809:1830	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	6	31	theme	high-calorie	771:782	arg1	diet					784:787	a high-calorie diet	769:787	a high-calorie diet	769:787	Our data revealed that LPS nebulization combined with a high-calorie diet resulted in significant changes in rats, such as slow weight gain, increased lung index, and aggravated lung inflammatory damage.					
35638590	8	32	theme	intestinal	1263:1272	arg1	microbiota					1274:1283	the intestinal microbiota	1259:1283	the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats	1259:1357	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	6	33	from	changes	813:819	arg1	rats					824:827	rats	824:827	rats	824:827	Our data revealed that LPS nebulization combined with a high-calorie diet resulted in significant changes in rats, such as slow weight gain, increased lung index, and aggravated lung inflammatory damage.					
35638590	3	34	theme	underlying	347:356	arg1	mechanisms					358:367	the underlying mechanisms	343:367	the underlying mechanisms	343:367	However, the underlying mechanisms remain unclear.					
35638590	11	35	theme	cell	1819:1822	arg1	balance					1824:1830	Th17/Treg cell balance	1809:1830	Th17/Treg cell balance	1809:1830	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	8	36	theme	high-throughput	1062:1076	arg1	sequencing					1078:1087	high-throughput sequencing	1062:1087	high-throughput sequencing of intestinal contents	1062:1110	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	1	37	theme	systemic	190:197	arg1	infections					208:217	the systemic or local infections	186:217	the systemic or local infections in the host	186:229	The intestinal flora plays an important role in the inflammatory response to the systemic or local infections in the host.					
35638590	8	38	theme	microbial	1196:1204	arg1	diversity					1206:1214	microbial diversity	1196:1214	microbial diversity	1196:1214	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	11	39	theme	inflammatory	1887:1898	arg1	damage					1900:1905	LPS-induced lung inflammatory damage	1870:1905	LPS-induced lung inflammatory damage	1870:1905	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	8	40	theme	acids	1337:1341	arg1	production					1305:1314	the production	1301:1314	the production of short-chain fatty acids (SCFAs) in rats	1301:1357	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	6	41	theme	lung	866:869	arg1	index					871:875	lung index	866:875	lung index	866:875	Our data revealed that LPS nebulization combined with a high-calorie diet resulted in significant changes in rats, such as slow weight gain, increased lung index, and aggravated lung inflammatory damage.					
35638590	7	42	theme	Th17/Treg	1032:1040	arg1	cells					1042:1046	Th17/Treg cells	1032:1046	Th17/Treg cells	1032:1046	Meanwhile, we found that the aggravation of LPS-induced pneumonia by a high-calorie diet disturbs the balance of Th17/Treg cells.					
35638590	11	43	theme	new	1928:1930	arg1	perspective					1932:1942	a new perspective	1926:1942	a new perspective on the pathogenesis of lung inflammation injury	1926:1990	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	11	44	theme	lung	1967:1970	arg1	injury					1985:1990	lung inflammation injury	1967:1990	lung inflammation injury	1967:1990	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	1	45	theme	local	202:206	arg1	infections					208:217	the systemic or local infections	186:217	the systemic or local infections in the host	186:229	The intestinal flora plays an important role in the inflammatory response to the systemic or local infections in the host.					
35638590	0	46	theme	LPS-induced	31:41	arg1	pneumonia					43:51	LPS-induced pneumonia	31:51	LPS-induced pneumonia	31:51	A high-calorie diet aggravates LPS-induced pneumonia by disturbing the gut microbiota and Th17/Treg balance.					
35638590	11	47	theme	immune	1836:1841	arg1	homeostasis					1843:1853	immune homeostasis	1836:1853	immune homeostasis	1836:1853	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	7	48	theme	cells	1042:1046	arg1	balance					1021:1027	the balance	1017:1027	the balance of Th17/Treg cells	1017:1046	Meanwhile, we found that the aggravation of LPS-induced pneumonia by a high-calorie diet disturbs the balance of Th17/Treg cells.					
35638590	6	49	theme	weight	843:848	arg1	gain					850:853	slow weight gain	838:853	slow weight gain	838:853	Our data revealed that LPS nebulization combined with a high-calorie diet resulted in significant changes in rats, such as slow weight gain, increased lung index, and aggravated lung inflammatory damage.					
35638590	11	50	theme	inflammation	1972:1983	arg1	injury					1985:1990	lung inflammation injury	1967:1990	lung inflammation injury	1967:1990	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	7	51	theme	high-calorie	990:1001	arg1	diet					1003:1006	a high-calorie diet	988:1006	a high-calorie diet	988:1006	Meanwhile, we found that the aggravation of LPS-induced pneumonia by a high-calorie diet disturbs the balance of Th17/Treg cells.					
35638590	10	52	theme	pneumonia	1610:1618	arg1	rats					1620:1623	pneumonia rats	1610:1623	pneumonia rats	1610:1623	More critically, the effects of a high-calorie diet were shown to be transmissible among pneumonia rats through cohousing microbiota transplantation.					
35638590	8	53	theme	contents	1103:1110	arg1	sequencing					1078:1087	high-throughput sequencing	1062:1087	high-throughput sequencing of intestinal contents	1062:1110	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	4	54	theme	LPS	448:450	arg1	atomization					452:462	LPS atomization	448:462	LPS atomization	448:462	Our previous studies demonstrated that a high-calorie diet and LPS atomization synergistically promoted lung inflammation injury in juvenile rats.					
35638590	1	55	from	infections	208:217	arg1	host					226:229	the host	222:229	the host	222:229	The intestinal flora plays an important role in the inflammatory response to the systemic or local infections in the host.					
35638590	1	56	theme	intestinal	113:122	arg1	flora					124:128	The intestinal flora	109:128	The intestinal flora	109:128	The intestinal flora plays an important role in the inflammatory response to the systemic or local infections in the host.					
35638590	8	57	theme	intestinal	1092:1101	arg1	contents					1103:1110	intestinal contents	1092:1110	intestinal contents	1092:1110	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	11	58	theme	high-calorie	1725:1736	arg1	diet					1738:1741	a high-calorie diet	1723:1741	a high-calorie diet	1723:1741	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	5	59	theme	pathogen-free	556:568	arg1	rats					579:582	specific pathogen-free juvenile rats	547:582	specific pathogen-free juvenile rats	547:582	In this study, specific pathogen-free juvenile rats were placed in a routine environment, and subjected to a high-calorie diet or LPS atomization in isolation as well as combination.					
35638590	2	60	dep	pneumonia	280:288	arg1	recovery					300:307	recovery	300:307	recovery	300:307	A high-calorie diet has been shown to aggravate pneumonia and delay recovery, especially in children.					
35638590	11	61	theme	LPS-induced	1870:1880	arg1	damage					1900:1905	LPS-induced lung inflammatory damage	1870:1905	LPS-induced lung inflammatory damage	1870:1905	Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.					
35638590	5	62	theme	juvenile	570:577	arg1	rats					579:582	specific pathogen-free juvenile rats	547:582	specific pathogen-free juvenile rats	547:582	In this study, specific pathogen-free juvenile rats were placed in a routine environment, and subjected to a high-calorie diet or LPS atomization in isolation as well as combination.					
35638590	8	63	theme	short-chain	1319:1329	arg1	acids					1337:1341	short-chain fatty acids	1319:1341	short-chain fatty acids (SCFAs)	1319:1349	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	8	63	theme	short-chain	1319:1329	arg1	SCFAs					1344:1348	SCFAs	1344:1348	SCFAs	1344:1348	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	6	64	theme	LPS	738:740	arg1	nebulization					742:753	LPS nebulization	738:753	LPS nebulization combined with a high-calorie diet	738:787	Our data revealed that LPS nebulization combined with a high-calorie diet resulted in significant changes in rats, such as slow weight gain, increased lung index, and aggravated lung inflammatory damage.					
35638590	4	65	theme	high-calorie	426:437	arg1	diet					439:442	a high-calorie diet	424:442	a high-calorie diet	424:442	Our previous studies demonstrated that a high-calorie diet and LPS atomization synergistically promoted lung inflammation injury in juvenile rats.					
35638590	7	66	theme	LPS-induced	963:973	arg1	pneumonia					975:983	LPS-induced pneumonia	963:983	LPS-induced pneumonia	963:983	Meanwhile, we found that the aggravation of LPS-induced pneumonia by a high-calorie diet disturbs the balance of Th17/Treg cells.					
35638590	9	67	theme	high-calorie	1502:1513	arg1	diet					1515:1518	a high-calorie diet	1500:1518	a high-calorie diet	1500:1518	Consequently, the levels of SCFAs, especially acetate, propionate, and butyrate, were significantly decreased following the intervention of a high-calorie diet.					
35638590	9	68	theme	SCFAs	1388:1392	arg1	levels					1378:1383	the levels	1374:1383	the levels of SCFAs, especially acetate, propionate, and butyrate,	1374:1439	Consequently, the levels of SCFAs, especially acetate, propionate, and butyrate, were significantly decreased following the intervention of a high-calorie diet.					
35638590	0	69	theme	gut	71:73	arg1	microbiota					75:84	the gut microbiota	67:84	the gut microbiota	67:84	A high-calorie diet aggravates LPS-induced pneumonia by disturbing the gut microbiota and Th17/Treg balance.					
35638590	2	70	theme	high-calorie	234:245	arg1	diet					247:250	A high-calorie diet	232:250	A high-calorie diet	232:250	A high-calorie diet has been shown to aggravate pneumonia and delay recovery, especially in children.					
35638590	7	71	theme	pneumonia	975:983	arg1	aggravation					948:958	the aggravation	944:958	the aggravation of LPS-induced pneumonia by a high-calorie diet	944:1006	Meanwhile, we found that the aggravation of LPS-induced pneumonia by a high-calorie diet disturbs the balance of Th17/Treg cells.					
35638590	1	72	theme	important	139:147	arg1	role					149:152	an important role	136:152	an important role	136:152	The intestinal flora plays an important role in the inflammatory response to the systemic or local infections in the host.					
35638590	6	73	theme	inflammatory	898:909	arg1	damage					911:916	lung inflammatory damage	893:916	lung inflammatory damage	893:916	Our data revealed that LPS nebulization combined with a high-calorie diet resulted in significant changes in rats, such as slow weight gain, increased lung index, and aggravated lung inflammatory damage.					
35638590	8	74	theme	fatty	1331:1335	arg1	acids					1337:1341	short-chain fatty acids	1319:1341	short-chain fatty acids (SCFAs)	1319:1349	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	8	74	theme	fatty	1331:1335	arg1	SCFAs					1344:1348	SCFAs	1344:1348	SCFAs	1344:1348	Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats.					
35638590	10	75	theme	diet	1568:1571	arg1	effects					1542:1548	the effects	1538:1548	the effects of a high-calorie diet	1538:1571	More critically, the effects of a high-calorie diet were shown to be transmissible among pneumonia rats through cohousing microbiota transplantation.					
35638590	10	75	theme	diet	1568:1571	arg1	transmissible					1590:1602	transmissible	1590:1602	transmissible	1590:1602	More critically, the effects of a high-calorie diet were shown to be transmissible among pneumonia rats through cohousing microbiota transplantation.					
37080073	0	0	theme	human	100:104	arg1	sweat					106:110	human sweat	100:110	human sweat	100:110	A molecularly imprinted electrochemical microneedle sensor for multiplexed metabolites detection in human sweat.					
37080073	1	1	theme	imprinted	175:183	arg1	platforms					197:205	inexpensive molecularly imprinted microneedle platforms	151:205	inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat	151:319	This article demonstrates an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat.					
37080073	8	2	theme	PON	1463:1465	arg1	sensor					1468:1473	need (PON) sensor	1457:1473	need (PON) sensor for sweat analytics	1457:1493	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	4	3	theme	layer	862:866	arg1	atop					868:871	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop	779:871	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer	779:909	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	1	4	theme	microneedle	185:195	arg1	platforms					197:205	inexpensive molecularly imprinted microneedle platforms	151:205	inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat	151:319	This article demonstrates an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat.					
37080073	8	5	from	dopamine	1362:1369	arg1	sample					1401:1406	a human sweat sample	1387:1406	a human sweat sample	1387:1406	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	2	6	theme	PDMS/CNT/CNC	541:552	arg1	PDMS					554:557	PDMS/CNT/CNC@PDMS	541:557	PDMS/CNT/CNC@PDMS	541:557	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	4	7	theme	PANI/CNT/CNC/AgNP	877:893	arg1	layer					905:909	the PANI/CNT/CNC/AgNP composite layer	873:909	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer	779:909	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	8	8	theme	human	1389:1393	arg1	sample					1401:1406	a human sweat sample	1387:1406	a human sweat sample	1387:1406	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	8	9	theme	pH	1345:1346	arg1	quantification					1327:1340	quantification	1327:1340	quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample	1327:1406	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	3	10	theme	pH	565:566	arg1	sensor					568:573	The pH sensor	561:573	The pH sensor	561:573	The pH sensor was comprised of a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer.					
37080073	8	11	theme	sensor	1468:1473	arg1	promise					1417:1423	promise	1417:1423	promise for use	1417:1431	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	8	11	theme	sensor	1468:1473	arg1	point					1448:1452	a wearable, point	1436:1452	point	1448:1452	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	8	12	from	lactate	1376:1382	arg1	sample					1401:1406	a human sweat sample	1387:1406	a human sweat sample	1387:1406	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	7	13	theme	pH	1229:1230	arg1	range					1232:1236	a pH range	1227:1236	a pH range of 4.25-10	1227:1247	The pH sensor accurately responded to a pH range of 4.25-10.					
37080073	8	14	theme	platforms	1282:1290	arg1	applicability					1254:1266	The applicability	1250:1266	The applicability of the sensor platforms	1250:1290	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	2	15	theme	composite	530:538	arg1	CNT					497:499	CNT	497:499	CNT	497:499	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	2	15	theme	composite	530:538	arg1	nanotube					487:494	a conductive PDMS/carbon nanotube	462:494	a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS)	462:558	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	2	16	dep	composite	530:538	arg1	PDMS					554:557	PDMS/CNT/CNC@PDMS	541:557	PDMS/CNT/CNC@PDMS	541:557	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	4	17	dep	epinephrine	683:693	arg1	sensors					718:724	sensors	718:724	sensors	718:724	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	1	18	theme	platforms	197:205	arg1	array					142:146	an array	139:146	an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat	139:319	This article demonstrates an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat.					
37080073	8	19	theme	sweat	1479:1483	arg1	analytics					1485:1493	sweat analytics	1479:1493	sweat analytics	1479:1493	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	4	20	theme	acid	826:829	arg1	atop					868:871	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop	779:871	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer	779:909	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	5	21	dep	rapidly	924:930	arg1	∼2 min					933:938	∼2 min	933:938	∼2 min	933:938	Each sensor rapidly (∼2 min) and selectively responded to their target analytes, with excellent precision between scans.					
37080073	6	22	theme	epinephrine	1071:1081	arg1	sensors					1106:1112	the epinephrine, dopamine, and lactate sensors	1067:1112	the epinephrine, dopamine, and lactate sensors	1067:1112	The limits of detection (LOD) for the epinephrine, dopamine, and lactate sensors were 0.0007 ± 0.0002 μM, 2.11 ± 0.05 nM, and 0.07 ± 0.07 mM, respectively.					
37080073	2	23	theme	PDMS/carbon	475:485	arg1	CNT					497:499	CNT	497:499	CNT	497:499	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	2	23	theme	PDMS/carbon	475:485	arg1	nanotube					487:494	a conductive PDMS/carbon nanotube	462:494	a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS)	462:558	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	5	24	theme	excellent	998:1006	arg1	precision					1008:1016	excellent precision	998:1016	excellent precision between scans	998:1030	Each sensor rapidly (∼2 min) and selectively responded to their target analytes, with excellent precision between scans.					
37080073	4	25	theme	composite	895:903	arg1	layer					905:909	the PANI/CNT/CNC/AgNP composite layer	873:909	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer	779:909	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	2	26	theme	conductive	464:473	arg1	CNT					497:499	CNT	497:499	CNT	497:499	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	2	26	theme	conductive	464:473	arg1	nanotube					487:494	a conductive PDMS/carbon nanotube	462:494	a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS)	462:558	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	4	27	theme	AuNP	856:859	arg1	atop					868:871	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop	779:871	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer	779:909	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	8	28	theme	wearable	1438:1445	arg1	promise					1417:1423	promise	1417:1423	promise for use	1417:1431	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	8	28	theme	wearable	1438:1445	arg1	point					1448:1452	a wearable, point	1436:1452	point	1448:1452	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	1	29	theme	multiplexed	215:225	arg1	detection					243:251	the multiplexed electrochemical detection	211:251	the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat	211:319	This article demonstrates an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat.					
37080073	0	30	theme	electrochemical	24:38	arg1	sensor					52:57	A molecularly imprinted electrochemical microneedle sensor	0:57	A molecularly imprinted electrochemical microneedle sensor for multiplexed metabolites	0:85	A molecularly imprinted electrochemical microneedle sensor for multiplexed metabolites detection in human sweat.					
37080073	1	31	theme	human	309:313	arg1	sweat					315:319	human sweat	309:319	human sweat	309:319	This article demonstrates an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat.					
37080073	1	32	from	lactate	287:293	arg1	sweat					315:319	human sweat	309:319	human sweat	309:319	This article demonstrates an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat.					
37080073	2	33	from	assembly	387:394	arg1	platform					441:448	a polydimethylsiloxane (PDMS) microneedle platform	399:448	a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS)	399:558	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	2	34	theme	multiplexed	326:336	arg1	sensors					338:344	The multiplexed sensors	322:344	The multiplexed sensors	322:344	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	1	35	theme	electrochemical	227:241	arg1	detection					243:251	the multiplexed electrochemical detection	211:251	the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat	211:319	This article demonstrates an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat.					
37080073	0	36	theme	imprinted	14:22	arg1	sensor					52:57	A molecularly imprinted electrochemical microneedle sensor	0:57	A molecularly imprinted electrochemical microneedle sensor for multiplexed metabolites	0:85	A molecularly imprinted electrochemical microneedle sensor for multiplexed metabolites detection in human sweat.					
37080073	5	37	theme	target	976:981	arg1	analytes					983:990	their target analytes	970:990	their target analytes	970:990	Each sensor rapidly (∼2 min) and selectively responded to their target analytes, with excellent precision between scans.					
37080073	2	38	theme	LbL	382:384	arg1	assembly					387:394	layer-by-layer (LbL) assembly	366:394	layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS)	366:558	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	3	39	theme	/CNT/CNC/silver	626:640	arg1	layer					672:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	The pH sensor was comprised of a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer.					
37080073	6	40	theme	detection	1047:1055	arg1	limits					1037:1042	The limits	1033:1042	The limits of detection (LOD) for the epinephrine, dopamine, and lactate sensors	1033:1112	The limits of detection (LOD) for the epinephrine, dopamine, and lactate sensors were 0.0007 ± 0.0002 μM, 2.11 ± 0.05 nM, and 0.07 ± 0.07 mM, respectively.					
37080073	6	40	theme	detection	1047:1055	arg1	0.0007 ± 0.0002 μM					1119:1136	0.0007 ± 0.0002 μM	1119:1136	0.0007 ± 0.0002 μM	1119:1136	The limits of detection (LOD) for the epinephrine, dopamine, and lactate sensors were 0.0007 ± 0.0002 μM, 2.11 ± 0.05 nM, and 0.07 ± 0.07 mM, respectively.					
37080073	3	41	theme	AgNP	656:659	arg1	layer					672:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	The pH sensor was comprised of a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer.					
37080073	2	42	theme	microneedle	429:439	arg1	platform					441:448	a polydimethylsiloxane (PDMS) microneedle platform	399:448	a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS)	399:558	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	0	43	theme	microneedle	40:50	arg1	sensor					52:57	A molecularly imprinted electrochemical microneedle sensor	0:57	A molecularly imprinted electrochemical microneedle sensor for multiplexed metabolites	0:85	A molecularly imprinted electrochemical microneedle sensor for multiplexed metabolites detection in human sweat.					
37080073	2	44	theme	@	553:553	arg1	PDMS					554:557	PDMS/CNT/CNC@PDMS	541:557	PDMS/CNT/CNC@PDMS	541:557	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	0	45	from	detection	87:95	arg1	sweat					106:110	human sweat	100:110	human sweat	100:110	A molecularly imprinted electrochemical microneedle sensor for multiplexed metabolites detection in human sweat.					
37080073	3	46	theme	pH-responsive	594:606	arg1	layer					672:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	The pH sensor was comprised of a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer.					
37080073	1	47	theme	pH	256:257	arg1	detection					243:251	the multiplexed electrochemical detection	211:251	the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat	211:319	This article demonstrates an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat.					
37080073	0	48	theme	multiplexed	63:73	arg1	metabolites					75:85	multiplexed metabolites	63:85	multiplexed metabolites	63:85	A molecularly imprinted electrochemical microneedle sensor for multiplexed metabolites detection in human sweat.					
37080073	7	49	theme	pH	1193:1194	arg1	sensor					1196:1201	The pH sensor	1189:1201	The pH sensor	1189:1201	The pH sensor accurately responded to a pH range of 4.25-10.					
37080073	1	50	dep	epinephrine	260:270	arg1	biomarkers					295:304	biomarkers	295:304	biomarkers	295:304	This article demonstrates an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat.					
37080073	4	51	theme	/gold	836:840	arg1	atop					868:871	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop	779:871	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer	779:909	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	8	52	theme	sensor	1275:1280	arg1	platforms					1282:1290	the sensor platforms	1271:1290	the sensor platforms	1271:1290	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	1	53	from	dopamine	273:280	arg1	sweat					315:319	human sweat	309:319	human sweat	309:319	This article demonstrates an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat.					
37080073	3	54	theme	nanoparticle	642:653	arg1	layer					672:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	The pH sensor was comprised of a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer.					
37080073	4	55	theme	nanoparticle	842:853	arg1	atop					868:871	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop	779:871	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer	779:909	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	2	56	theme	layer-by-layer	366:379	arg1	assembly					387:394	layer-by-layer (LbL) assembly	366:394	layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS)	366:558	The multiplexed sensors were fabricated via layer-by-layer (LbL) assembly on a polydimethylsiloxane (PDMS) microneedle platform coated with a conductive PDMS/carbon nanotube (CNT)/cellulose nanocrystal (CNC) composite (PDMS/CNT/CNC@PDMS).					
37080073	8	57	from	epinephrine	1349:1359	arg1	sample					1401:1406	a human sweat sample	1387:1406	a human sweat sample	1387:1406	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	4	58	theme	lactate-imprinted	779:795	arg1	PBA					832:834	PBA	832:834	PBA	832:834	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	4	58	theme	lactate-imprinted	779:795	arg1	acid					826:829	lactate-imprinted PANI-co-3-aminophenylboronic acid	779:829	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer	779:909	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	3	59	theme	PANI	621:624	arg1	layer					672:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	The pH sensor was comprised of a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer.					
37080073	6	60	theme	lactate	1098:1104	arg1	sensors					1106:1112	the epinephrine, dopamine, and lactate sensors	1067:1112	the epinephrine, dopamine, and lactate sensors	1067:1112	The limits of detection (LOD) for the epinephrine, dopamine, and lactate sensors were 0.0007 ± 0.0002 μM, 2.11 ± 0.05 nM, and 0.07 ± 0.07 mM, respectively.					
37080073	4	61	theme	additional	742:751	arg1	epinephrine					753:763	an additional epinephrine	739:763	an additional epinephrine	739:763	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	1	62	from	epinephrine	260:270	arg1	sweat					315:319	human sweat	309:319	human sweat	309:319	This article demonstrates an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat.					
37080073	8	63	theme	sweat	1395:1399	arg1	sample					1401:1406	a human sweat sample	1387:1406	a human sweat sample	1387:1406	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	7	64	theme	4.25-10	1241:1247	arg1	range					1232:1236	a pH range	1227:1236	a pH range of 4.25-10	1227:1247	The pH sensor accurately responded to a pH range of 4.25-10.					
37080073	3	65	theme	composite	662:670	arg1	layer					672:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	The pH sensor was comprised of a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer.					
37080073	3	66	theme	polyaniline	608:618	arg1	layer					672:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer	592:676	The pH sensor was comprised of a pH-responsive polyaniline (PANI)/CNT/CNC/silver nanoparticle (AgNP) composite layer.					
37080073	1	67	theme	inexpensive	151:161	arg1	platforms					197:205	inexpensive molecularly imprinted microneedle platforms	151:205	inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat	151:319	This article demonstrates an array of inexpensive molecularly imprinted microneedle platforms for the multiplexed electrochemical detection of pH, epinephrine, dopamine, and lactate biomarkers in human sweat.					
37080073	4	68	dep	atop	868:871	arg1	layer					905:909	the PANI/CNT/CNC/AgNP composite layer	873:909	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer	779:909	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	8	69	theme	need	1457:1460	arg1	sensor					1468:1473	need (PON) sensor	1457:1473	need (PON) sensor for sweat analytics	1457:1493	The applicability of the sensor platforms were successfully verified through quantification of pH, epinephrine, dopamine, and lactate in a human sweat sample, showing promise for use as a wearable, point of need (PON) sensor for sweat analytics.					
37080073	4	70	theme	PANI-co-3-aminophenylboronic	797:824	arg1	PBA					832:834	PBA	832:834	PBA	832:834	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	4	70	theme	PANI-co-3-aminophenylboronic	797:824	arg1	acid					826:829	lactate-imprinted PANI-co-3-aminophenylboronic acid	779:829	lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer	779:909	The epinephrine, dopamine, and lactate sensors consisted of an additional epinephrine, dopamine, or lactate-imprinted PANI-co-3-aminophenylboronic acid (PBA)/gold nanoparticle (AuNP) layer atop the PANI/CNT/CNC/AgNP composite layer.					
37080073	6	71	theme	dopamine	1084:1091	arg1	sensors					1106:1112	the epinephrine, dopamine, and lactate sensors	1067:1112	the epinephrine, dopamine, and lactate sensors	1067:1112	The limits of detection (LOD) for the epinephrine, dopamine, and lactate sensors were 0.0007 ± 0.0002 μM, 2.11 ± 0.05 nM, and 0.07 ± 0.07 mM, respectively.					
35716796	8	0	theme	growth	1202:1207	arg1	ability					1209:1215	growth ability	1202:1215	growth ability	1202:1215	The results indicated that cells in low-concentration RSFMA composite gel presented better growth ability, proliferation ability and osteogenic differentiation ability.					
35716796	1	1	from	challenge	185:193	arg1	field					202:206	the field	198:206	the field of stomatology	198:221	The construction of suitable biomaterials for pulp regeneration has always been a major challenge in the field of stomatology.					
35716796	7	2	theme	high	1081:1084	arg1	concentrations					1086:1099	high concentrations	1081:1099	high concentrations of RSFMA	1081:1108	In contrast to MeHA hydrogels, hDPSCs were encapsulated in hydrogels either in the absence or presence of high concentrations of RSFMA.					
35716796	4	3	theme	human	629:633	arg1	cells					652:656	encapsulated human dental pulp stem cells	616:656	encapsulated human dental pulp stem cells (hDPSCs)	616:665	The biocompatibility of encapsulated human dental pulp stem cells (hDPSCs) was subsequently investigated.					
35716796	4	3	theme	human	629:633	arg1	hDPSCs					659:664	hDPSCs	659:664	hDPSCs	659:664	The biocompatibility of encapsulated human dental pulp stem cells (hDPSCs) was subsequently investigated.					
35716796	6	4	theme	cross-linking	949:961	arg1	efficiency					963:972	higher cross-linking efficiency	942:972	higher cross-linking efficiency	942:972	Furthermore, the low concentration of RSFMA hydrogel in the composite hydrogel had higher cross-linking efficiency.					
35716796	9	5	theme	injectable	1285:1294	arg1	hydrogel					1342:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel	1280:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel	1280:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel shows great potential in the field of dental pulp tissue engineering.					
35716796	3	6	theme	composite	523:531	arg1	hydrogel					533:540	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	4	7	theme	encapsulated	616:627	arg1	cells					652:656	encapsulated human dental pulp stem cells	616:656	encapsulated human dental pulp stem cells (hDPSCs)	616:665	The biocompatibility of encapsulated human dental pulp stem cells (hDPSCs) was subsequently investigated.					
35716796	4	7	theme	encapsulated	616:627	arg1	hDPSCs					659:664	hDPSCs	659:664	hDPSCs	659:664	The biocompatibility of encapsulated human dental pulp stem cells (hDPSCs) was subsequently investigated.					
35716796	5	8	theme	scaffolds	747:755	arg1	size					735:738	the pore size	726:738	the pore size of the scaffolds	726:755	With the addition of RSFMA, the pore size of the scaffolds became more regular with negligible change in porosity and exhibited excellent mechanical properties.					
35716796	6	9	theme	higher	942:947	arg1	efficiency					963:972	higher cross-linking efficiency	942:972	higher cross-linking efficiency	942:972	Furthermore, the low concentration of RSFMA hydrogel in the composite hydrogel had higher cross-linking efficiency.					
35716796	3	10	theme	physicochemical	564:578	arg1	properties					580:589	its physicochemical properties	560:589	its physicochemical properties	560:589	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	2	11	theme	injectable	296:305	arg1	hydrogels					307:315	injectable hydrogels	296:315	injectable hydrogels	296:315	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
35716796	4	12	theme	pulp	642:645	arg1	cells					652:656	encapsulated human dental pulp stem cells	616:656	encapsulated human dental pulp stem cells (hDPSCs)	616:665	The biocompatibility of encapsulated human dental pulp stem cells (hDPSCs) was subsequently investigated.					
35716796	4	12	theme	pulp	642:645	arg1	hDPSCs					659:664	hDPSCs	659:664	hDPSCs	659:664	The biocompatibility of encapsulated human dental pulp stem cells (hDPSCs) was subsequently investigated.					
35716796	9	13	theme	pulp	1396:1399	arg1	field					1380:1384	the field	1376:1384	the field of dental pulp tissue engineering	1376:1418	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel shows great potential in the field of dental pulp tissue engineering.					
35716796	8	14	dep	ability	1209:1215	arg1	ability					1271:1277	ability	1271:1277	ability	1271:1277	The results indicated that cells in low-concentration RSFMA composite gel presented better growth ability, proliferation ability and osteogenic differentiation ability.					
35716796	8	15	theme	low-concentration	1147:1163	arg1	gel					1181:1183	low-concentration RSFMA composite gel	1147:1183	low-concentration RSFMA composite gel	1147:1183	The results indicated that cells in low-concentration RSFMA composite gel presented better growth ability, proliferation ability and osteogenic differentiation ability.					
35716796	2	16	theme	system	288:293	arg1	anatomy					262:268	the complex and irregular anatomy	236:268	the complex and irregular anatomy of the root canal system	236:293	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
35716796	3	17	theme	acid	511:514	arg1	hydrogel					533:540	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	9	18	dep	pulp	1396:1399	arg1	engineering					1408:1418	tissue engineering	1401:1418	dental pulp tissue engineering	1389:1418	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel shows great potential in the field of dental pulp tissue engineering.					
35716796	2	19	theme	cell	354:357	arg1	attention					341:349	extensive attention	331:349	extensive attention	331:349	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
35716796	2	19	theme	cell	354:357	arg1	carriers					359:366	cell carriers	354:366	cell carriers in dental pulp regeneration	354:394	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
35716796	3	20	link	photocrosslinked	430:445	arg1	MeHA					517:520	MeHA	517:520	MeHA	517:520	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	3	20	link	photocrosslinked	430:445	arg1	acid					511:514	photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid	430:514	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	0	21	link	Photocross-linked	0:16	arg1	hydrogel					47:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel	0:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel	0:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel loaded with hDPSC for pulp regeneration.					
35716796	2	22	theme	canal	282:286	arg1	system					288:293	the root canal system	273:293	the root canal system	273:293	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
35716796	1	23	theme	major	179:183	arg1	construction					101:112	The construction	97:112	The construction of suitable biomaterials for pulp regeneration	97:159	The construction of suitable biomaterials for pulp regeneration has always been a major challenge in the field of stomatology.					
35716796	1	23	theme	major	179:183	arg1	challenge					185:193	a major challenge	177:193	a major challenge in the field of stomatology	177:221	The construction of suitable biomaterials for pulp regeneration has always been a major challenge in the field of stomatology.					
35716796	4	24	theme	dental	635:640	arg1	cells					652:656	encapsulated human dental pulp stem cells	616:656	encapsulated human dental pulp stem cells (hDPSCs)	616:665	The biocompatibility of encapsulated human dental pulp stem cells (hDPSCs) was subsequently investigated.					
35716796	4	24	theme	dental	635:640	arg1	hDPSCs					659:664	hDPSCs	659:664	hDPSCs	659:664	The biocompatibility of encapsulated human dental pulp stem cells (hDPSCs) was subsequently investigated.					
35716796	6	25	theme	composite	919:927	arg1	hydrogel					929:936	the composite hydrogel	915:936	the composite hydrogel	915:936	Furthermore, the low concentration of RSFMA hydrogel in the composite hydrogel had higher cross-linking efficiency.					
35716796	5	26	theme	mechanical	836:845	arg1	properties					847:856	excellent mechanical properties	826:856	excellent mechanical properties	826:856	With the addition of RSFMA, the pore size of the scaffolds became more regular with negligible change in porosity and exhibited excellent mechanical properties.					
35716796	2	27	theme	root	277:280	arg1	system					288:293	the root canal system	273:293	the root canal system	273:293	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
35716796	7	28	dep	absence	1058:1064	arg1	the					1054:1056	the	1054:1056	the	1054:1056	In contrast to MeHA hydrogels, hDPSCs were encapsulated in hydrogels either in the absence or presence of high concentrations of RSFMA.					
35716796	3	29	theme	fibroin	468:474	arg1	MeHA					517:520	MeHA	517:520	MeHA	517:520	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	3	29	theme	fibroin	468:474	arg1	acid					511:514	photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid	430:514	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	0	30	theme	silk	18:21	arg1	hydrogel					47:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel	0:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel	0:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel loaded with hDPSC for pulp regeneration.					
35716796	2	31	from	carriers	359:366	arg1	regeneration					383:394	dental pulp regeneration	371:394	dental pulp regeneration	371:394	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
35716796	7	32	theme	concentrations	1086:1099	arg1	presence					1069:1076	presence	1069:1076	presence	1069:1076	In contrast to MeHA hydrogels, hDPSCs were encapsulated in hydrogels either in the absence or presence of high concentrations of RSFMA.					
35716796	7	32	theme	concentrations	1086:1099	arg1	absence					1058:1064	absence	1058:1064	absence	1058:1064	In contrast to MeHA hydrogels, hDPSCs were encapsulated in hydrogels either in the absence or presence of high concentrations of RSFMA.					
35716796	3	33	theme	photocrosslinked	430:445	arg1	MeHA					517:520	MeHA	517:520	MeHA	517:520	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	3	33	theme	photocrosslinked	430:445	arg1	acid					511:514	photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid	430:514	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	0	34	theme	Photocross-linked	0:16	arg1	hydrogel					47:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel	0:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel	0:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel loaded with hDPSC for pulp regeneration.					
35716796	7	35	theme	RSFMA	1104:1108	arg1	concentrations					1086:1099	high concentrations	1081:1099	high concentrations of RSFMA	1081:1108	In contrast to MeHA hydrogels, hDPSCs were encapsulated in hydrogels either in the absence or presence of high concentrations of RSFMA.					
35716796	9	36	theme	dental	1389:1394	arg1	pulp					1396:1399	dental pulp tissue engineering	1389:1418	dental pulp tissue engineering	1389:1418	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel shows great potential in the field of dental pulp tissue engineering.					
35716796	3	37	theme	methacrylylated	447:461	arg1	MeHA					517:520	MeHA	517:520	MeHA	517:520	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	3	37	theme	methacrylylated	447:461	arg1	acid					511:514	photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid	430:514	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	0	38	theme	acid	42:45	arg1	hydrogel					47:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel	0:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel	0:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel loaded with hDPSC for pulp regeneration.					
35716796	8	39	theme	proliferation	1218:1230	arg1	ability					1232:1238	proliferation ability	1218:1238	proliferation ability	1218:1238	The results indicated that cells in low-concentration RSFMA composite gel presented better growth ability, proliferation ability and osteogenic differentiation ability.					
35716796	5	40	theme	pore	730:733	arg1	size					735:738	the pore size	726:738	the pore size of the scaffolds	726:755	With the addition of RSFMA, the pore size of the scaffolds became more regular with negligible change in porosity and exhibited excellent mechanical properties.					
35716796	9	41	link	photocrosslinked	1296:1311	arg1	hydrogel					1342:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel	1280:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel	1280:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel shows great potential in the field of dental pulp tissue engineering.					
35716796	9	42	theme	fibroin/hyaluronic	1318:1335	arg1	hydrogel					1342:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel	1280:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel	1280:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel shows great potential in the field of dental pulp tissue engineering.					
35716796	0	43	theme	fibroin/hyaluronic	23:40	arg1	hydrogel					47:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel	0:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel	0:54	Photocross-linked silk fibroin/hyaluronic acid hydrogel loaded with hDPSC for pulp regeneration.					
35716796	9	44	theme	silk	1313:1316	arg1	hydrogel					1342:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel	1280:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel	1280:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel shows great potential in the field of dental pulp tissue engineering.					
35716796	1	45	theme	suitable	117:124	arg1	biomaterials					126:137	suitable biomaterials	117:137	suitable biomaterials	117:137	The construction of suitable biomaterials for pulp regeneration has always been a major challenge in the field of stomatology.					
35716796	7	46	dep	hydrogels	1034:1042	arg1	either					1044:1049	either	1044:1049	either	1044:1049	In contrast to MeHA hydrogels, hDPSCs were encapsulated in hydrogels either in the absence or presence of high concentrations of RSFMA.					
35716796	3	47	theme	/methacrylylated	483:498	arg1	MeHA					517:520	MeHA	517:520	MeHA	517:520	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	3	47	theme	/methacrylylated	483:498	arg1	acid					511:514	photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid	430:514	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	9	48	theme	tissue	1401:1406	arg1	engineering					1408:1418	tissue engineering	1401:1418	dental pulp tissue engineering	1389:1418	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel shows great potential in the field of dental pulp tissue engineering.					
35716796	6	49	theme	hydrogel	903:910	arg1	concentration					880:892	the low concentration	872:892	the low concentration of RSFMA hydrogel in the composite hydrogel	872:936	Furthermore, the low concentration of RSFMA hydrogel in the composite hydrogel had higher cross-linking efficiency.					
35716796	8	50	from	cells	1138:1142	arg1	gel					1181:1183	low-concentration RSFMA composite gel	1147:1183	low-concentration RSFMA composite gel	1147:1183	The results indicated that cells in low-concentration RSFMA composite gel presented better growth ability, proliferation ability and osteogenic differentiation ability.					
35716796	1	51	theme	stomatology	211:221	arg1	field					202:206	the field	198:206	the field of stomatology	198:221	The construction of suitable biomaterials for pulp regeneration has always been a major challenge in the field of stomatology.					
35716796	3	52	theme	silk	463:466	arg1	MeHA					517:520	MeHA	517:520	MeHA	517:520	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	3	52	theme	silk	463:466	arg1	acid					511:514	photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid	430:514	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	3	53	theme	injectable	419:428	arg1	hydrogel					533:540	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	6	54	theme	RSFMA	897:901	arg1	hydrogel					903:910	RSFMA hydrogel	897:910	RSFMA hydrogel	897:910	Furthermore, the low concentration of RSFMA hydrogel in the composite hydrogel had higher cross-linking efficiency.					
35716796	2	55	theme	complex	240:246	arg1	anatomy					262:268	the complex and irregular anatomy	236:268	the complex and irregular anatomy of the root canal system	236:293	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
35716796	3	56	theme	RSFMA	477:481	arg1	MeHA					517:520	MeHA	517:520	MeHA	517:520	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	3	56	theme	RSFMA	477:481	arg1	acid					511:514	photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid	430:514	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	6	57	from	concentration	880:892	arg1	hydrogel					929:936	the composite hydrogel	915:936	the composite hydrogel	915:936	Furthermore, the low concentration of RSFMA hydrogel in the composite hydrogel had higher cross-linking efficiency.					
35716796	5	58	theme	excellent	826:834	arg1	properties					847:856	excellent mechanical properties	826:856	excellent mechanical properties	826:856	With the addition of RSFMA, the pore size of the scaffolds became more regular with negligible change in porosity and exhibited excellent mechanical properties.					
35716796	9	59	theme	photocrosslinked	1296:1311	arg1	hydrogel					1342:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel	1280:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel	1280:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel shows great potential in the field of dental pulp tissue engineering.					
35716796	1	60	theme	biomaterials	126:137	arg1	construction					101:112	The construction	97:112	The construction of suitable biomaterials for pulp regeneration	97:159	The construction of suitable biomaterials for pulp regeneration has always been a major challenge in the field of stomatology.					
35716796	1	60	theme	biomaterials	126:137	arg1	challenge					185:193	a major challenge	177:193	a major challenge in the field of stomatology	177:221	The construction of suitable biomaterials for pulp regeneration has always been a major challenge in the field of stomatology.					
35716796	4	61	theme	stem	647:650	arg1	cells					652:656	encapsulated human dental pulp stem cells	616:656	encapsulated human dental pulp stem cells (hDPSCs)	616:665	The biocompatibility of encapsulated human dental pulp stem cells (hDPSCs) was subsequently investigated.					
35716796	4	61	theme	stem	647:650	arg1	hDPSCs					659:664	hDPSCs	659:664	hDPSCs	659:664	The biocompatibility of encapsulated human dental pulp stem cells (hDPSCs) was subsequently investigated.					
35716796	7	62	theme	MeHA	990:993	arg1	hydrogels					995:1003	MeHA hydrogels	990:1003	MeHA hydrogels	990:1003	In contrast to MeHA hydrogels, hDPSCs were encapsulated in hydrogels either in the absence or presence of high concentrations of RSFMA.					
35716796	5	63	theme	RSFMA	719:723	arg1	addition					707:714	the addition	703:714	the addition of RSFMA	703:723	With the addition of RSFMA, the pore size of the scaffolds became more regular with negligible change in porosity and exhibited excellent mechanical properties.					
35716796	5	64	from	change	793:798	arg1	porosity					803:810	porosity	803:810	porosity	803:810	With the addition of RSFMA, the pore size of the scaffolds became more regular with negligible change in porosity and exhibited excellent mechanical properties.					
35716796	9	65	theme	great	1357:1361	arg1	potential					1363:1371	great potential	1357:1371	great potential	1357:1371	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel shows great potential in the field of dental pulp tissue engineering.					
35716796	9	66	theme	acid	1337:1340	arg1	hydrogel					1342:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel	1280:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel	1280:1349	This injectable photocrosslinked silk fibroin/hyaluronic acid hydrogel shows great potential in the field of dental pulp tissue engineering.					
35716796	7	67	dep	hydrogels	995:1003	arg1	contrast					978:985	contrast	978:985	contrast	978:985	In contrast to MeHA hydrogels, hDPSCs were encapsulated in hydrogels either in the absence or presence of high concentrations of RSFMA.					
35716796	8	68	theme	osteogenic	1244:1253	arg1	differentiation					1255:1269	osteogenic differentiation	1244:1269	osteogenic differentiation	1244:1269	The results indicated that cells in low-concentration RSFMA composite gel presented better growth ability, proliferation ability and osteogenic differentiation ability.					
35716796	8	69	theme	composite	1171:1179	arg1	gel					1181:1183	low-concentration RSFMA composite gel	1147:1183	low-concentration RSFMA composite gel	1147:1183	The results indicated that cells in low-concentration RSFMA composite gel presented better growth ability, proliferation ability and osteogenic differentiation ability.					
35716796	6	70	theme	low	876:878	arg1	concentration					880:892	the low concentration	872:892	the low concentration of RSFMA hydrogel in the composite hydrogel	872:936	Furthermore, the low concentration of RSFMA hydrogel in the composite hydrogel had higher cross-linking efficiency.					
35716796	1	71	theme	pulp	143:146	arg1	regeneration					148:159	pulp regeneration	143:159	pulp regeneration	143:159	The construction of suitable biomaterials for pulp regeneration has always been a major challenge in the field of stomatology.					
35716796	4	72	theme	cells	652:656	arg1	biocompatibility					596:611	The biocompatibility	592:611	The biocompatibility of encapsulated human dental pulp stem cells (hDPSCs)	592:665	The biocompatibility of encapsulated human dental pulp stem cells (hDPSCs) was subsequently investigated.					
35716796	8	73	theme	RSFMA	1165:1169	arg1	gel					1181:1183	low-concentration RSFMA composite gel	1147:1183	low-concentration RSFMA composite gel	1147:1183	The results indicated that cells in low-concentration RSFMA composite gel presented better growth ability, proliferation ability and osteogenic differentiation ability.					
35716796	2	74	theme	extensive	331:339	arg1	attention					341:349	extensive attention	331:349	extensive attention	331:349	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
35716796	2	74	theme	extensive	331:339	arg1	carriers					359:366	cell carriers	354:366	cell carriers in dental pulp regeneration	354:394	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
35716796	3	75	theme	hyaluronic	500:509	arg1	MeHA					517:520	MeHA	517:520	MeHA	517:520	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	3	75	theme	hyaluronic	500:509	arg1	acid					511:514	photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid	430:514	an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel	416:540	Here, we developed an injectable photocrosslinked methacrylylated silk fibroin (RSFMA)/methacrylylated hyaluronic acid (MeHA) composite hydrogel and characterized its physicochemical properties.					
35716796	0	76	theme	pulp	78:81	arg1	regeneration					83:94	pulp regeneration	78:94	pulp regeneration	78:94	Photocross-linked silk fibroin/hyaluronic acid hydrogel loaded with hDPSC for pulp regeneration.					
35716796	6	77	contain	had	938:940	arg1	concentration					880:892	the low concentration	872:892	the low concentration of RSFMA hydrogel in the composite hydrogel	872:936	Furthermore, the low concentration of RSFMA hydrogel in the composite hydrogel had higher cross-linking efficiency.					
35716796	6	77	contain	had	938:940	arg2	efficiency					963:972	higher cross-linking efficiency	942:972	higher cross-linking efficiency	942:972	Furthermore, the low concentration of RSFMA hydrogel in the composite hydrogel had higher cross-linking efficiency.					
35716796	2	78	theme	pulp	378:381	arg1	regeneration					383:394	dental pulp regeneration	371:394	dental pulp regeneration	371:394	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
35716796	5	79	theme	negligible	782:791	arg1	change					793:798	negligible change	782:798	negligible change in porosity	782:810	With the addition of RSFMA, the pore size of the scaffolds became more regular with negligible change in porosity and exhibited excellent mechanical properties.					
35716796	2	80	theme	irregular	252:260	arg1	anatomy					262:268	the complex and irregular anatomy	236:268	the complex and irregular anatomy of the root canal system	236:293	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
35716796	2	81	theme	dental	371:376	arg1	regeneration					383:394	dental pulp regeneration	371:394	dental pulp regeneration	371:394	Considering the complex and irregular anatomy of the root canal system, injectable hydrogels have received extensive attention as cell carriers in dental pulp regeneration.					
37012442	14	0	theme	milk	2134:2137	arg1	TS					2127:2128	TS	2127:2128	TS	2127:2128	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	14	0	theme	milk	2134:2137	arg1	solids					2119:2124	the total solids	2109:2124	the total solids (TS) of milk	2109:2137	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	10	1	dep	tended	1555:1560	arg1	greater					1568:1574	greater	1568:1574	greater	1568:1574	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	13	2	theme	P	2060:2060	arg1	<					2062:2062	P < 0.05	2060:2067	P < 0.05	2060:2067	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	2	3	theme	experimental	339:350	arg1	period					352:357	experimental period 1	339:359	experimental period 1	339:359	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	6	4	theme	detergent	1218:1226	arg1	fiber					1228:1232	neutral detergent fiber	1210:1232	neutral detergent fiber	1210:1232	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	2	5	theme	HSC	558:560	arg1	diet					563:566	high SC (HSC) diet	549:566	high SC (HSC) diet (0.8 g SC/head/day; n=10)	549:592	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	5	theme	HSC	558:560	arg1	SC/head/day					575:585	0.8 g SC/head/day	569:585	0.8 g SC/head/day	569:585	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	3	6	theme	experimental	853:864	arg1	days					918:921	4 days	916:921	4 days for data and sample collection	916:952	For experimental period 2, 4 ewes from each group were randomly selected and were individually housed in metabolism crates with 7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection.					
37012442	3	6	theme	experimental	853:864	arg1	periods					866:872	7-day experimental periods	847:872	7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection	847:952	For experimental period 2, 4 ewes from each group were randomly selected and were individually housed in metabolism crates with 7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection.					
37012442	3	6	theme	experimental	853:864	arg1	days					886:889	3 days	884:889	3 days for crate adaptation	884:910	For experimental period 2, 4 ewes from each group were randomly selected and were individually housed in metabolism crates with 7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection.					
37012442	8	7	theme	rumen	1370:1374	arg1	pH					1382:1383	the rumen fluid pH	1366:1383	the rumen fluid pH of lactating ewe supplemented with SC	1366:1421	A significant increase was observed for the rumen fluid pH of lactating ewe supplemented with SC.					
37012442	12	8	theme	Milk	1828:1831	arg1	%					1837:1837	Milk fat % and yield	1828:1847	%	1837:1837	Milk fat % and yield in the SC treatment groups were also greater (P = 0.05).					
37012442	16	9	theme	other	2478:2482	arg1	concentrations					2501:2514	all other serum metabolite concentrations	2474:2514	all other serum metabolite concentrations of lactating ewes in between treatment groups	2474:2560	With the exception of aspartate aminotransferase and alkaline phosphatase, no differences were detected in all other serum metabolite concentrations of lactating ewes in between treatment groups.					
37012442	8	10	theme	ewe	1398:1400	arg1	pH					1382:1383	the rumen fluid pH	1366:1383	the rumen fluid pH of lactating ewe supplemented with SC	1366:1421	A significant increase was observed for the rumen fluid pH of lactating ewe supplemented with SC.					
37012442	2	11	theme	experimental	606:617	arg1	week					639:642	1 week	637:642	1 week for dietary and pen adaptation	637:673	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	11	theme	experimental	606:617	arg1	periods					619:625	9-week experimental periods	599:625	9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection	599:716	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	11	theme	experimental	606:617	arg1	weeks					681:685	8 weeks	679:685	8 weeks for data and sample collection	679:716	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	10	12	theme	average	1617:1623	arg1	gain					1631:1634	average daily gain	1617:1634	average daily gain	1617:1634	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	12	13	from	yield	1843:1847	arg1	groups					1869:1874	the SC treatment groups	1852:1874	the SC treatment groups	1852:1874	Milk fat % and yield in the SC treatment groups were also greater (P = 0.05).					
37012442	12	14	from	%	1837:1837	arg1	groups					1869:1874	the SC treatment groups	1852:1874	the SC treatment groups	1852:1874	Milk fat % and yield in the SC treatment groups were also greater (P = 0.05).					
37012442	1	15	theme	Saccharomyces	183:195	arg1	SC					209:210	SC	209:210	SC	209:210	This study aimed to investigate the impact of supplementing two levels of Saccharomyces cerevisiae (SC) during suckling period on performance and serum metabolites of Awassi ewes.					
37012442	1	15	theme	Saccharomyces	183:195	arg1	cerevisiae					197:206	Saccharomyces cerevisiae	183:206	Saccharomyces cerevisiae (SC)	183:211	This study aimed to investigate the impact of supplementing two levels of Saccharomyces cerevisiae (SC) during suckling period on performance and serum metabolites of Awassi ewes.					
37012442	14	16	theme	SC	2250:2251	arg1	groups					2263:2268	SC treatment groups	2250:2268	SC treatment groups	2250:2268	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	10	17	theme	total	1640:1644	arg1	gain					1653:1656	total weight gain	1640:1656	total weight gain	1640:1656	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	17	18	contain	had	2655:2657	arg1	supplementation					2607:2621	SC supplementation	2604:2621	SC supplementation with varying levels in the diet	2604:2653	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	17	18	contain	had	2655:2657	arg2	effect					2678:2683	a similar positive effect	2659:2683	a similar positive effect	2659:2683	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	16	19	theme	ewes	2529:2532	arg1	concentrations					2501:2514	all other serum metabolite concentrations	2474:2514	all other serum metabolite concentrations of lactating ewes in between treatment groups	2474:2560	With the exception of aspartate aminotransferase and alkaline phosphatase, no differences were detected in all other serum metabolite concentrations of lactating ewes in between treatment groups.					
37012442	14	20	theme	solids	2119:2124	arg1	%					2104:2104	%	2104:2104	% of the total solids (TS) of milk	2104:2137	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	14	20	theme	solids	2119:2124	arg1	TS					2127:2128	TS	2127:2128	TS	2127:2128	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	14	20	theme	solids	2119:2124	arg1	solids					2119:2124	the total solids	2109:2124	the total solids (TS) of milk	2109:2137	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	15	21	theme	CON	2356:2358	arg1	diets					2360:2364	CON diets	2356:2364	CON diets	2356:2364	Energy-corrected milk values were greater (P < 0.05) in HSC diet compared to LSC and CON diets.					
37012442	15	22	theme	Energy-corrected	2271:2286	arg1	values					2293:2298	Energy-corrected milk values	2271:2298	Energy-corrected milk values	2271:2298	Energy-corrected milk values were greater (P < 0.05) in HSC diet compared to LSC and CON diets.					
37012442	11	23	theme	treatment	1745:1753	arg1	groups					1755:1760	the SC treatment groups	1738:1760	the SC treatment groups	1738:1760	Lactating ewes in the SC treatment groups produced more milk per day (P ≤ 0.05) than those in the CON diet.					
37012442	0	24	from	period	71:76	arg1	performance					81:91	performance	81:91	performance of Awassi ewes	81:106	Effect of Saccharomyces cerevisiae supplementation during the suckling period on performance of Awassi ewes.					
37012442	3	25	from	group	763:767	arg1	ewes					748:751	4 ewes	746:751	4 ewes from each group	746:767	For experimental period 2, 4 ewes from each group were randomly selected and were individually housed in metabolism crates with 7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection.					
37012442	17	26	dep	performance	2693:2703	arg1	some					2688:2691	some	2688:2691	some	2688:2691	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	17	27	theme	similar	2661:2667	arg1	effect					2678:2683	a similar positive effect	2659:2683	a similar positive effect	2659:2683	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	13	28	theme	P	2007:2007	arg1	>					2009:2009	P > 0.05	2007:2014	P > 0.05	2007:2014	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	28	theme	P	2007:2007	arg1	groups					1999:2004	all groups	1995:2004	all groups (P > 0.05) while lactose	1995:2029	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	2	29	theme	SC	504:505	arg1	SC/head/day					525:535	0.4 g SC/head/day; n=10	519:541	0.4 g SC/head/day; n=10	519:541	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	29	theme	SC	504:505	arg1	diet					513:516	low SC (LSC) diet	500:516	low SC (LSC) diet (0.4 g SC/head/day; n=10)	500:542	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	14	30	theme	TS	2210:2211	arg1	yields					2213:2218	TS yields	2210:2218	TS yields	2210:2218	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	5	31	dep	higher	1081:1086	arg1	<					1091:1091	P < 0.05	1089:1096	P < 0.05	1089:1096	Digestibility of DM was higher (P < 0.05) for SC treatment groups.					
37012442	14	32	dep	higher	2143:2148	arg1	<					2196:2196	P < 0.05	2194:2201	P < 0.05	2194:2201	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	5	33	theme	DM	1074:1075	arg1	Digestibility					1057:1069	Digestibility	1057:1069	Digestibility of DM	1057:1075	Digestibility of DM was higher (P < 0.05) for SC treatment groups.					
37012442	4	34	theme	matter	1018:1023	arg1	intake					1030:1035	dry matter (DM) intake	1014:1035	dry matter (DM) intake of ewes (P = 0.03)	1014:1054	The results showed that the supplementation of SC improved dry matter (DM) intake of ewes (P = 0.03).					
37012442	17	35	theme	physiological	2709:2721	arg1	parameters					2723:2732	physiological parameters	2709:2732	physiological parameters	2709:2732	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	9	36	theme	weight	1463:1468	arg1	change					1470:1475	the live weight change	1454:1475	the live weight change of lactating ewes in all treatment groups	1454:1517	No difference was detected in the live weight change of lactating ewes in all treatment groups.					
37012442	15	37	theme	HSC	2327:2329	arg1	diet					2331:2334	HSC diet	2327:2334	HSC diet	2327:2334	Energy-corrected milk values were greater (P < 0.05) in HSC diet compared to LSC and CON diets.					
37012442	17	38	theme	Awassi	2747:2752	arg1	ewes					2754:2757	lactating Awassi ewes	2737:2757	lactating Awassi ewes	2737:2757	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	10	39	theme	=	1687:1687	arg1	P					1685:1685	P = 0.05	1685:1692	P = 0.05	1685:1692	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	16	40	theme	alkaline	2420:2427	arg1	phosphatase					2429:2439	alkaline phosphatase	2420:2439	alkaline phosphatase	2420:2439	With the exception of aspartate aminotransferase and alkaline phosphatase, no differences were detected in all other serum metabolite concentrations of lactating ewes in between treatment groups.					
37012442	10	41	theme	body	1530:1533	arg1	weight					1535:1540	The final body weight	1520:1540	The final body weight of the lambs	1520:1553	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	16	42	located	detected	2462:2469	arg2	differences					2445:2455	no differences	2442:2455	no differences	2442:2455	With the exception of aspartate aminotransferase and alkaline phosphatase, no differences were detected in all other serum metabolite concentrations of lactating ewes in between treatment groups.					
37012442	16	42	located	detected	2462:2469	arg1	concentrations					2501:2514	all other serum metabolite concentrations	2474:2514	all other serum metabolite concentrations of lactating ewes in between treatment groups	2474:2560	With the exception of aspartate aminotransferase and alkaline phosphatase, no differences were detected in all other serum metabolite concentrations of lactating ewes in between treatment groups.					
37012442	9	43	located	detected	1442:1449	arg1	change					1470:1475	the live weight change	1454:1475	the live weight change of lactating ewes in all treatment groups	1454:1517	No difference was detected in the live weight change of lactating ewes in all treatment groups.					
37012442	9	43	located	detected	1442:1449	arg2	difference					1427:1436	No difference	1424:1436	No difference	1424:1436	No difference was detected in the live weight change of lactating ewes in all treatment groups.					
37012442	3	44	theme	experimental	723:734	arg1	period					736:741	experimental period 2	723:743	experimental period 2	723:743	For experimental period 2, 4 ewes from each group were randomly selected and were individually housed in metabolism crates with 7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection.					
37012442	2	45	theme	groups	464:469	arg1	groups					464:469	three equal treatment groups	442:469	three equal treatment groups	442:469	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	45	theme	groups	464:469	arg1	one					435:437	one	435:437	one	435:437	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	46	theme	low	500:502	arg1	SC/head/day					525:535	0.4 g SC/head/day; n=10	519:541	0.4 g SC/head/day; n=10	519:541	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	46	theme	low	500:502	arg1	diet					513:516	low SC (LSC) diet	500:516	low SC (LSC) diet (0.4 g SC/head/day; n=10)	500:542	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	17	47	theme	lambs	2769:2773	arg1	performance					2693:2703	performance	2693:2703	performance	2693:2703	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	17	47	theme	lambs	2769:2773	arg1	parameters					2723:2732	physiological parameters	2709:2732	physiological parameters	2709:2732	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	16	48	theme	aminotransferase	2399:2414	arg1	exception					2376:2384	the exception	2372:2384	the exception of aspartate aminotransferase and alkaline phosphatase	2372:2439	With the exception of aspartate aminotransferase and alkaline phosphatase, no differences were detected in all other serum metabolite concentrations of lactating ewes in between treatment groups.					
37012442	6	49	theme	treatment	1131:1139	arg1	groups					1141:1146	The SC treatment groups	1124:1146	The SC treatment groups	1124:1146	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	7	50	theme	balance	1298:1304	arg1	parameters					1306:1315	N balance parameters	1296:1315	N balance parameters of ewes	1296:1323	Addition of SC improved (P < 0.05) N balance parameters of ewes.					
37012442	9	51	theme	ewes	1490:1493	arg1	change					1470:1475	the live weight change	1454:1475	the live weight change of lactating ewes in all treatment groups	1454:1517	No difference was detected in the live weight change of lactating ewes in all treatment groups.					
37012442	10	52	dep	higher	1677:1682	arg1	P					1685:1685	P = 0.05	1685:1692	P = 0.05	1685:1692	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	4	53	theme	ewes	1040:1043	arg1	intake					1030:1035	dry matter (DM) intake	1014:1035	dry matter (DM) intake of ewes (P = 0.03)	1014:1054	The results showed that the supplementation of SC improved dry matter (DM) intake of ewes (P = 0.03).					
37012442	10	54	theme	SC	1699:1700	arg1	groups					1712:1717	SC treatment groups	1699:1717	SC treatment groups	1699:1717	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	0	55	theme	Awassi	96:101	arg1	ewes					103:106	Awassi ewes	96:106	Awassi ewes	96:106	Effect of Saccharomyces cerevisiae supplementation during the suckling period on performance of Awassi ewes.					
37012442	3	56	theme	crate	895:899	arg1	adaptation					901:910	crate adaptation	895:910	crate adaptation	895:910	For experimental period 2, 4 ewes from each group were randomly selected and were individually housed in metabolism crates with 7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection.					
37012442	12	57	theme	SC	1856:1857	arg1	groups					1869:1874	the SC treatment groups	1852:1874	the SC treatment groups	1852:1874	Milk fat % and yield in the SC treatment groups were also greater (P = 0.05).					
37012442	3	58	theme	data	927:930	arg1	collection					943:952	data and sample collection	927:952	collection	943:952	For experimental period 2, 4 ewes from each group were randomly selected and were individually housed in metabolism crates with 7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection.					
37012442	14	59	theme	P	2194:2194	arg1	<					2196:2196	P < 0.05	2194:2201	P < 0.05	2194:2201	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	4	60	theme	DM	1026:1027	arg1	intake					1030:1035	dry matter (DM) intake	1014:1035	dry matter (DM) intake of ewes (P = 0.03)	1014:1054	The results showed that the supplementation of SC improved dry matter (DM) intake of ewes (P = 0.03).					
37012442	2	61	dep	periods	326:332	arg1	assigned					423:430	assigned	423:430	were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection	409:716	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	5	62	theme	treatment	1106:1114	arg1	groups					1116:1121	SC treatment groups	1103:1121	SC treatment groups	1103:1121	Digestibility of DM was higher (P < 0.05) for SC treatment groups.					
37012442	2	63	theme	nursing	365:371	arg1	ewes					380:383	30 nursing Awassi ewes	362:383	30 nursing Awassi ewes with their single lambs	362:407	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	6	64	theme	protein	1201:1207	arg1	digestibility					1178:1190	digestibility	1178:1190	digestibility of crude protein, neutral detergent fiber, and acid detergent fiber	1178:1258	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	13	65	theme	solid-not-fat	1967:1979	arg1	SNF					1982:1984	SNF	1982:1984	SNF	1982:1984	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	65	theme	solid-not-fat	1967:1979	arg1	lactose					1954:1960	lactose	1954:1960	lactose	1954:1960	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	65	theme	solid-not-fat	1967:1979	arg1	%					1935:1935	%	1935:1935	% of milk protein, lactose, and solid-not-fat (SNF)	1935:1985	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	65	theme	solid-not-fat	1967:1979	arg1	protein					1945:1951	milk protein	1940:1951	milk protein	1940:1951	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	65	theme	solid-not-fat	1967:1979	arg1	solid-not-fat					1967:1979	solid-not-fat	1967:1979	solid-not-fat (SNF)	1967:1985	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	2	66	theme	SC	554:555	arg1	diet					563:566	high SC (HSC) diet	549:566	high SC (HSC) diet (0.8 g SC/head/day; n=10)	549:592	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	66	theme	SC	554:555	arg1	SC/head/day					575:585	0.8 g SC/head/day	569:585	0.8 g SC/head/day	569:585	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	5	67	theme	P	1089:1089	arg1	<					1091:1091	P < 0.05	1089:1096	P < 0.05	1089:1096	Digestibility of DM was higher (P < 0.05) for SC treatment groups.					
37012442	0	68	theme	Saccharomyces	10:22	arg1	supplementation					35:49	Saccharomyces cerevisiae supplementation	10:49	Saccharomyces cerevisiae supplementation	10:49	Effect of Saccharomyces cerevisiae supplementation during the suckling period on performance of Awassi ewes.					
37012442	1	69	theme	ewes	283:286	arg1	performance					239:249	performance	239:249	performance	239:249	This study aimed to investigate the impact of supplementing two levels of Saccharomyces cerevisiae (SC) during suckling period on performance and serum metabolites of Awassi ewes.					
37012442	1	69	theme	ewes	283:286	arg1	metabolites					261:271	serum metabolites	255:271	serum metabolites	255:271	This study aimed to investigate the impact of supplementing two levels of Saccharomyces cerevisiae (SC) during suckling period on performance and serum metabolites of Awassi ewes.					
37012442	7	70	theme	P	1286:1286	arg1	<					1288:1288	P < 0.05	1286:1293	P < 0.05	1286:1293	Addition of SC improved (P < 0.05) N balance parameters of ewes.					
37012442	2	71	theme	equal	448:452	arg1	groups					464:469	three equal treatment groups	442:469	three equal treatment groups	442:469	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	0	72	theme	supplementation	35:49	arg1	Effect					0:5	Effect	0:5	Effect of Saccharomyces cerevisiae supplementation during the suckling period on performance of Awassi ewes.	0:107	Effect of Saccharomyces cerevisiae supplementation during the suckling period on performance of Awassi ewes.					
37012442	3	73	theme	sample	936:941	arg1	collection					943:952	data and sample collection	927:952	collection	943:952	For experimental period 2, 4 ewes from each group were randomly selected and were individually housed in metabolism crates with 7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection.					
37012442	10	74	theme	lambs	1549:1553	arg1	weight					1535:1540	The final body weight	1520:1540	The final body weight of the lambs	1520:1553	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	6	75	theme	tendency	1154:1161	arg1	improvement					1163:1173	a tendency improvement	1152:1173	a tendency improvement	1152:1173	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	12	76	dep	greater	1886:1892	arg1	=					1897:1897	=	1897:1897	=	1897:1897	Milk fat % and yield in the SC treatment groups were also greater (P = 0.05).					
37012442	10	77	theme	SC	1580:1581	arg1	groups					1593:1598	SC treatment groups	1580:1598	SC treatment groups	1580:1598	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	6	78	theme	detergent	1244:1252	arg1	fiber					1254:1258	acid detergent fiber	1239:1258	acid detergent fiber	1239:1258	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	7	79	dep	improved	1276:1283	arg1	<					1288:1288	P < 0.05	1286:1293	P < 0.05	1286:1293	Addition of SC improved (P < 0.05) N balance parameters of ewes.					
37012442	0	80	dep	Saccharomyces	10:22	arg1	cerevisiae					24:33	cerevisiae	24:33	cerevisiae	24:33	Effect of Saccharomyces cerevisiae supplementation during the suckling period on performance of Awassi ewes.					
37012442	15	81	dep	greater	2305:2311	arg1	<					2316:2316	P < 0.05	2314:2321	P < 0.05	2314:2321	Energy-corrected milk values were greater (P < 0.05) in HSC diet compared to LSC and CON diets.					
37012442	2	82	theme	sample	700:705	arg1	collection					707:716	data and sample collection	691:716	collection	707:716	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	16	83	theme	treatment	2545:2553	arg1	groups					2555:2560	treatment groups	2545:2560	treatment groups	2545:2560	With the exception of aspartate aminotransferase and alkaline phosphatase, no differences were detected in all other serum metabolite concentrations of lactating ewes in between treatment groups.					
37012442	13	84	theme	SC	2074:2075	arg1	groups					2087:2092	SC treatment groups	2074:2092	SC treatment groups	2074:2092	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	85	theme	milk	1940:1943	arg1	protein					1945:1951	milk protein	1940:1951	milk protein	1940:1951	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	2	86	theme	data	691:694	arg1	collection					707:716	data and sample collection	691:716	collection	707:716	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	10	87	theme	CON	1603:1605	arg1	group					1607:1611	CON group	1603:1611	CON group	1603:1611	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	6	88	theme	fiber	1228:1232	arg1	digestibility					1178:1190	digestibility	1178:1190	digestibility of crude protein, neutral detergent fiber, and acid detergent fiber	1178:1258	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	2	89	with	ewes	380:383	arg1	lambs					403:407	their single lambs	390:407	their single lambs	390:407	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	3	90	theme	7-day	847:851	arg1	days					918:921	4 days	916:921	4 days for data and sample collection	916:952	For experimental period 2, 4 ewes from each group were randomly selected and were individually housed in metabolism crates with 7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection.					
37012442	3	90	theme	7-day	847:851	arg1	periods					866:872	7-day experimental periods	847:872	7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection	847:952	For experimental period 2, 4 ewes from each group were randomly selected and were individually housed in metabolism crates with 7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection.					
37012442	3	90	theme	7-day	847:851	arg1	days					886:889	3 days	884:889	3 days for crate adaptation	884:910	For experimental period 2, 4 ewes from each group were randomly selected and were individually housed in metabolism crates with 7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection.					
37012442	13	91	located	found	1926:1930	arg1	SNF					1982:1984	SNF	1982:1984	SNF	1982:1984	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	91	located	found	1926:1930	arg1	protein					1945:1951	milk protein	1940:1951	milk protein	1940:1951	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	91	located	found	1926:1930	arg2	differences					1909:1919	No differences	1906:1919	No differences	1906:1919	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	91	located	found	1926:1930	arg1	lactose					1954:1960	lactose	1954:1960	lactose	1954:1960	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	91	located	found	1926:1930	arg1	%					1935:1935	%	1935:1935	% of milk protein, lactose, and solid-not-fat (SNF)	1935:1985	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	91	located	found	1926:1930	arg1	solid-not-fat					1967:1979	solid-not-fat	1967:1979	solid-not-fat (SNF)	1967:1985	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	6	92	theme	neutral	1210:1216	arg1	fiber					1228:1232	neutral detergent fiber	1210:1232	neutral detergent fiber	1210:1232	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	1	93	theme	serum	255:259	arg1	metabolites					261:271	serum metabolites	255:271	serum metabolites	255:271	This study aimed to investigate the impact of supplementing two levels of Saccharomyces cerevisiae (SC) during suckling period on performance and serum metabolites of Awassi ewes.					
37012442	11	94	theme	more	1771:1774	arg1	milk					1776:1779	more milk	1771:1779	more milk	1771:1779	Lactating ewes in the SC treatment groups produced more milk per day (P ≤ 0.05) than those in the CON diet.					
37012442	6	95	contain	had	1148:1150	arg1	groups					1141:1146	The SC treatment groups	1124:1146	The SC treatment groups	1124:1146	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	6	95	contain	had	1148:1150	arg2	improvement					1163:1173	a tendency improvement	1152:1173	a tendency improvement	1152:1173	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	10	96	theme	daily	1625:1629	arg1	gain					1631:1634	average daily gain	1617:1634	average daily gain	1617:1634	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	13	97	theme	SNF	2035:2037	arg1	yields					2039:2044	SNF yields	2035:2044	SNF yields	2035:2044	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	98	theme	treatment	2077:2085	arg1	groups					2087:2092	SC treatment groups	2074:2092	SC treatment groups	2074:2092	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	11	99	theme	≤	1792:1792	arg1	P					1790:1790	P ≤ 0.05	1790:1797	P ≤ 0.05	1790:1797	Lactating ewes in the SC treatment groups produced more milk per day (P ≤ 0.05) than those in the CON diet.					
37012442	11	99	theme	≤	1792:1792	arg1	day					1785:1787	day	1785:1787	day (P ≤ 0.05)	1785:1798	Lactating ewes in the SC treatment groups produced more milk per day (P ≤ 0.05) than those in the CON diet.					
37012442	16	100	theme	metabolite	2490:2499	arg1	concentrations					2501:2514	all other serum metabolite concentrations	2474:2514	all other serum metabolite concentrations of lactating ewes in between treatment groups	2474:2560	With the exception of aspartate aminotransferase and alkaline phosphatase, no differences were detected in all other serum metabolite concentrations of lactating ewes in between treatment groups.					
37012442	2	101	theme	9-week	599:604	arg1	week					639:642	1 week	637:642	1 week for dietary and pen adaptation	637:673	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	101	theme	9-week	599:604	arg1	periods					619:625	9-week experimental periods	599:625	9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection	599:716	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	101	theme	9-week	599:604	arg1	weeks					681:685	8 weeks	679:685	8 weeks for data and sample collection	679:716	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	9	102	theme	lactating	1480:1488	arg1	ewes					1490:1493	lactating ewes	1480:1493	lactating ewes	1480:1493	No difference was detected in the live weight change of lactating ewes in all treatment groups.					
37012442	17	103	from	levels	2636:2641	arg1	diet					2650:2653	the diet	2646:2653	the diet	2646:2653	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	16	104	theme	serum	2484:2488	arg1	concentrations					2501:2514	all other serum metabolite concentrations	2474:2514	all other serum metabolite concentrations of lactating ewes in between treatment groups	2474:2560	With the exception of aspartate aminotransferase and alkaline phosphatase, no differences were detected in all other serum metabolite concentrations of lactating ewes in between treatment groups.					
37012442	2	105	theme	g	523:523	arg1	SC/head/day					525:535	0.4 g SC/head/day; n=10	519:541	0.4 g SC/head/day; n=10	519:541	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	105	theme	g	523:523	arg1	diet					513:516	low SC (LSC) diet	500:516	low SC (LSC) diet (0.4 g SC/head/day; n=10)	500:542	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	14	106	theme	treatment	2253:2261	arg1	groups					2263:2268	SC treatment groups	2250:2268	SC treatment groups	2250:2268	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	10	107	theme	weight	1646:1651	arg1	gain					1653:1656	total weight gain	1640:1656	total weight gain	1640:1656	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	15	108	theme	milk	2288:2291	arg1	values					2293:2298	Energy-corrected milk values	2271:2298	Energy-corrected milk values	2271:2298	Energy-corrected milk values were greater (P < 0.05) in HSC diet compared to LSC and CON diets.					
37012442	1	109	theme	cerevisiae	197:206	arg1	levels					173:178	two levels	169:178	two levels of Saccharomyces cerevisiae (SC)	169:211	This study aimed to investigate the impact of supplementing two levels of Saccharomyces cerevisiae (SC) during suckling period on performance and serum metabolites of Awassi ewes.					
37012442	15	110	theme	P	2314:2314	arg1	<					2316:2316	P < 0.05	2314:2321	P < 0.05	2314:2321	Energy-corrected milk values were greater (P < 0.05) in HSC diet compared to LSC and CON diets.					
37012442	10	111	theme	final	1524:1528	arg1	weight					1535:1540	The final body weight	1520:1540	The final body weight of the lambs	1520:1553	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	16	112	theme	lactating	2519:2527	arg1	ewes					2529:2532	lactating ewes	2519:2532	lactating ewes in between treatment groups	2519:2560	With the exception of aspartate aminotransferase and alkaline phosphatase, no differences were detected in all other serum metabolite concentrations of lactating ewes in between treatment groups.					
37012442	14	113	theme	total	2113:2117	arg1	TS					2127:2128	TS	2127:2128	TS	2127:2128	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	14	113	theme	total	2113:2117	arg1	solids					2119:2124	the total solids	2109:2124	the total solids (TS) of milk	2109:2137	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	11	114	theme	Lactating	1720:1728	arg1	ewes					1730:1733	Lactating ewes	1720:1733	Lactating ewes in the SC treatment groups	1720:1760	Lactating ewes in the SC treatment groups produced more milk per day (P ≤ 0.05) than those in the CON diet.					
37012442	4	115	theme	dry	1014:1016	arg1	intake					1030:1035	dry matter (DM) intake	1014:1035	dry matter (DM) intake of ewes (P = 0.03)	1014:1054	The results showed that the supplementation of SC improved dry matter (DM) intake of ewes (P = 0.03).					
37012442	6	116	theme	SC	1128:1129	arg1	groups					1141:1146	The SC treatment groups	1124:1146	The SC treatment groups	1124:1146	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	17	117	theme	lactating	2737:2745	arg1	ewes					2754:2757	lactating Awassi ewes	2737:2757	lactating Awassi ewes	2737:2757	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	17	118	theme	positive	2669:2676	arg1	effect					2678:2683	a similar positive effect	2659:2683	a similar positive effect	2659:2683	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	7	119	theme	ewes	1320:1323	arg1	parameters					1306:1315	N balance parameters	1296:1315	N balance parameters of ewes	1296:1323	Addition of SC improved (P < 0.05) N balance parameters of ewes.					
37012442	9	120	theme	live	1458:1461	arg1	change					1470:1475	the live weight change	1454:1475	the live weight change of lactating ewes in all treatment groups	1454:1517	No difference was detected in the live weight change of lactating ewes in all treatment groups.					
37012442	17	121	theme	ewes	2754:2757	arg1	performance					2693:2703	performance	2693:2703	performance	2693:2703	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	17	121	theme	ewes	2754:2757	arg1	parameters					2723:2732	physiological parameters	2709:2732	physiological parameters	2709:2732	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	16	122	theme	phosphatase	2429:2439	arg1	exception					2376:2384	the exception	2372:2384	the exception of aspartate aminotransferase and alkaline phosphatase	2372:2439	With the exception of aspartate aminotransferase and alkaline phosphatase, no differences were detected in all other serum metabolite concentrations of lactating ewes in between treatment groups.					
37012442	8	123	theme	lactating	1388:1396	arg1	ewe					1398:1400	lactating ewe	1388:1400	lactating ewe supplemented with SC	1388:1421	A significant increase was observed for the rumen fluid pH of lactating ewe supplemented with SC.					
37012442	8	124	theme	fluid	1376:1380	arg1	pH					1382:1383	the rumen fluid pH	1366:1383	the rumen fluid pH of lactating ewe supplemented with SC	1366:1421	A significant increase was observed for the rumen fluid pH of lactating ewe supplemented with SC.					
37012442	11	125	from	ewes	1730:1733	arg1	groups					1755:1760	the SC treatment groups	1738:1760	the SC treatment groups	1738:1760	Lactating ewes in the SC treatment groups produced more milk per day (P ≤ 0.05) than those in the CON diet.					
37012442	4	126	theme	SC	1002:1003	arg1	supplementation					983:997	the supplementation	979:997	the supplementation of SC	979:1003	The results showed that the supplementation of SC improved dry matter (DM) intake of ewes (P = 0.03).					
37012442	11	127	theme	CON	1818:1820	arg1	diet					1822:1825	the CON diet	1814:1825	the CON diet	1814:1825	Lactating ewes in the SC treatment groups produced more milk per day (P ≤ 0.05) than those in the CON diet.					
37012442	7	128	theme	N	1296:1296	arg1	parameters					1306:1315	N balance parameters	1296:1315	N balance parameters of ewes	1296:1323	Addition of SC improved (P < 0.05) N balance parameters of ewes.					
37012442	2	129	theme	treatment	454:462	arg1	groups					464:469	three equal treatment groups	442:469	three equal treatment groups	442:469	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	130	theme	high	549:552	arg1	diet					563:566	high SC (HSC) diet	549:566	high SC (HSC) diet (0.8 g SC/head/day; n=10)	549:592	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	130	theme	high	549:552	arg1	SC/head/day					575:585	0.8 g SC/head/day	569:585	0.8 g SC/head/day	569:585	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	10	131	theme	treatment	1702:1710	arg1	groups					1712:1717	SC treatment groups	1699:1717	SC treatment groups	1699:1717	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	0	132	theme	ewes	103:106	arg1	performance					81:91	performance	81:91	performance of Awassi ewes	81:106	Effect of Saccharomyces cerevisiae supplementation during the suckling period on performance of Awassi ewes.					
37012442	12	133	theme	treatment	1859:1867	arg1	groups					1869:1874	the SC treatment groups	1852:1874	the SC treatment groups	1852:1874	Milk fat % and yield in the SC treatment groups were also greater (P = 0.05).					
37012442	4	134	theme	=	1048:1048	arg1	P					1046:1046	P = 0.03	1046:1053	P = 0.03	1046:1053	The results showed that the supplementation of SC improved dry matter (DM) intake of ewes (P = 0.03).					
37012442	4	134	theme	=	1048:1048	arg1	ewes					1040:1043	ewes	1040:1043	ewes (P = 0.03)	1040:1054	The results showed that the supplementation of SC improved dry matter (DM) intake of ewes (P = 0.03).					
37012442	17	135	theme	varying	2628:2634	arg1	levels					2636:2641	varying levels	2628:2641	varying levels in the diet	2628:2653	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	17	136	theme	SC	2604:2605	arg1	supplementation					2607:2621	SC supplementation	2604:2621	SC supplementation with varying levels in the diet	2604:2653	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	1	137	from	impact	145:150	arg1	performance					239:249	performance	239:249	performance	239:249	This study aimed to investigate the impact of supplementing two levels of Saccharomyces cerevisiae (SC) during suckling period on performance and serum metabolites of Awassi ewes.					
37012442	1	137	from	impact	145:150	arg1	metabolites					261:271	serum metabolites	255:271	serum metabolites	255:271	This study aimed to investigate the impact of supplementing two levels of Saccharomyces cerevisiae (SC) during suckling period on performance and serum metabolites of Awassi ewes.					
37012442	2	138	dep	SC/head/day	575:585	arg1	n=10					588:591	n=10	588:591	n=10	588:591	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	139	theme	control	474:480	arg1	diet					482:485	a control diet	472:485	a control diet (CON; n=10)	472:497	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	139	theme	control	474:480	arg1	CON					488:490	CON; n=10	488:496	CON; n=10	488:496	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	5	140	theme	SC	1103:1104	arg1	groups					1116:1121	SC treatment groups	1103:1121	SC treatment groups	1103:1121	Digestibility of DM was higher (P < 0.05) for SC treatment groups.					
37012442	2	141	theme	Awassi	373:378	arg1	ewes					380:383	30 nursing Awassi ewes	362:383	30 nursing Awassi ewes with their single lambs	362:407	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	11	142	theme	SC	1742:1743	arg1	groups					1755:1760	the SC treatment groups	1738:1760	the SC treatment groups	1738:1760	Lactating ewes in the SC treatment groups produced more milk per day (P ≤ 0.05) than those in the CON diet.					
37012442	6	143	theme	crude	1195:1199	arg1	protein					1201:1207	crude protein	1195:1207	crude protein	1195:1207	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	1	144	theme	Awassi	276:281	arg1	ewes					283:286	Awassi ewes	276:286	Awassi ewes	276:286	This study aimed to investigate the impact of supplementing two levels of Saccharomyces cerevisiae (SC) during suckling period on performance and serum metabolites of Awassi ewes.					
37012442	14	145	theme	CON	2183:2185	arg1	diets					2187:2191	CON diets	2183:2191	CON diets	2183:2191	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	12	146	theme	fat	1833:1835	arg1	%					1837:1837	Milk fat % and yield	1828:1847	%	1837:1837	Milk fat % and yield in the SC treatment groups were also greater (P = 0.05).					
37012442	2	147	theme	single	396:401	arg1	lambs					403:407	their single lambs	390:407	their single lambs	390:407	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	16	148	theme	aspartate	2389:2397	arg1	aminotransferase					2399:2414	aspartate aminotransferase	2389:2414	aspartate aminotransferase	2389:2414	With the exception of aspartate aminotransferase and alkaline phosphatase, no differences were detected in all other serum metabolite concentrations of lactating ewes in between treatment groups.					
37012442	13	149	theme	protein	1945:1951	arg1	SNF					1982:1984	SNF	1982:1984	SNF	1982:1984	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	149	theme	protein	1945:1951	arg1	lactose					1954:1960	lactose	1954:1960	lactose	1954:1960	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	149	theme	protein	1945:1951	arg1	%					1935:1935	%	1935:1935	% of milk protein, lactose, and solid-not-fat (SNF)	1935:1985	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	149	theme	protein	1945:1951	arg1	protein					1945:1951	milk protein	1940:1951	milk protein	1940:1951	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	149	theme	protein	1945:1951	arg1	solid-not-fat					1967:1979	solid-not-fat	1967:1979	solid-not-fat (SNF)	1967:1985	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	150	dep	greater	2051:2057	arg1	<					2062:2062	P < 0.05	2060:2067	P < 0.05	2060:2067	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	9	151	theme	treatment	1502:1510	arg1	groups					1512:1517	all treatment groups	1498:1517	all treatment groups	1498:1517	No difference was detected in the live weight change of lactating ewes in all treatment groups.					
37012442	0	152	theme	suckling	62:69	arg1	period					71:76	the suckling period	58:76	the suckling period on performance of Awassi ewes	58:106	Effect of Saccharomyces cerevisiae supplementation during the suckling period on performance of Awassi ewes.					
37012442	13	153	theme	lactose	1954:1960	arg1	SNF					1982:1984	SNF	1982:1984	SNF	1982:1984	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	153	theme	lactose	1954:1960	arg1	lactose					1954:1960	lactose	1954:1960	lactose	1954:1960	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	153	theme	lactose	1954:1960	arg1	%					1935:1935	%	1935:1935	% of milk protein, lactose, and solid-not-fat (SNF)	1935:1985	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	153	theme	lactose	1954:1960	arg1	protein					1945:1951	milk protein	1940:1951	milk protein	1940:1951	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	13	153	theme	lactose	1954:1960	arg1	solid-not-fat					1967:1979	solid-not-fat	1967:1979	solid-not-fat (SNF)	1967:1985	No differences were found in % of milk protein, lactose, and solid-not-fat (SNF) between all groups (P > 0.05) while lactose and SNF yields were greater (P < 0.05) for SC treatment groups.					
37012442	17	154	with	supplementation	2607:2621	arg1	levels					2636:2641	varying levels	2628:2641	varying levels in the diet	2628:2653	In conclusion, this study indicates that SC supplementation with varying levels in the diet had a similar positive effect on some performance and physiological parameters of lactating Awassi ewes and their lambs.					
37012442	10	155	theme	treatment	1583:1591	arg1	groups					1593:1598	SC treatment groups	1580:1598	SC treatment groups	1580:1598	The final body weight of the lambs tended to be greater for SC treatment groups vs CON group but average daily gain and total weight gain were significantly higher (P = 0.05) for SC treatment groups.					
37012442	6	156	theme	fiber	1254:1258	arg1	digestibility					1178:1190	digestibility	1178:1190	digestibility of crude protein, neutral detergent fiber, and acid detergent fiber	1178:1258	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	7	157	theme	SC	1273:1274	arg1	Addition					1261:1268	Addition	1261:1268	Addition of SC	1261:1274	Addition of SC improved (P < 0.05) N balance parameters of ewes.					
37012442	14	158	theme	HSC	2154:2156	arg1	diet					2158:2161	HSC diet	2154:2161	HSC diet	2154:2161	However, % of the total solids (TS) of milk was higher for HSC diet compared to LSC and CON diets (P < 0.05) while TS yields were significantly higher for SC treatment groups.					
37012442	2	159	theme	pen	660:662	arg1	adaptation					664:673	dietary and pen adaptation	648:673	adaptation	664:673	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	8	160	theme	significant	1328:1338	arg1	increase					1340:1347	A significant increase	1326:1347	A significant increase	1326:1347	A significant increase was observed for the rumen fluid pH of lactating ewe supplemented with SC.					
37012442	2	161	theme	g	573:573	arg1	diet					563:566	high SC (HSC) diet	549:566	high SC (HSC) diet (0.8 g SC/head/day; n=10)	549:592	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	161	theme	g	573:573	arg1	SC/head/day					575:585	0.8 g SC/head/day	569:585	0.8 g SC/head/day	569:585	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	2	162	theme	experimental	313:324	arg1	periods					326:332	two experimental periods	309:332	two experimental periods	309:332	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37012442	6	163	theme	acid	1239:1242	arg1	fiber					1254:1258	acid detergent fiber	1239:1258	acid detergent fiber	1239:1258	The SC treatment groups had a tendency improvement in digestibility of crude protein, neutral detergent fiber, and acid detergent fiber.					
37012442	1	164	theme	suckling	220:227	arg1	period					229:234	suckling period	220:234	suckling period	220:234	This study aimed to investigate the impact of supplementing two levels of Saccharomyces cerevisiae (SC) during suckling period on performance and serum metabolites of Awassi ewes.					
37012442	9	165	from	change	1470:1475	arg1	groups					1512:1517	all treatment groups	1498:1517	all treatment groups	1498:1517	No difference was detected in the live weight change of lactating ewes in all treatment groups.					
37012442	3	166	theme	metabolism	824:833	arg1	crates					835:840	metabolism crates	824:840	metabolism crates	824:840	For experimental period 2, 4 ewes from each group were randomly selected and were individually housed in metabolism crates with 7-day experimental periods including 3 days for crate adaptation and 4 days for data and sample collection.					
37012442	2	167	theme	dietary	648:654	arg1	adaptation					664:673	dietary and pen adaptation	648:673	adaptation	664:673	The study comprised two experimental periods, for experimental period 1, 30 nursing Awassi ewes with their single lambs were randomly assigned to one of three equal treatment groups: a control diet (CON; n=10), low SC (LSC) diet (0.4 g SC/head/day; n=10), and high SC (HSC) diet (0.8 g SC/head/day; n=10) with 9-week experimental periods including 1 week for dietary and pen adaptation and 8 weeks for data and sample collection.					
37085452	6	0	from	Force	875:879	arg1	GTFCC					901:905	GTFCC	901:905	GTFCC	901:905	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	6	0	from	Force	875:879	arg1	Control					892:898	Cholera Control	884:898	Cholera Control (GTFCC)	884:906	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	2	1	theme	Health	321:326	arg1	program					356:362	the World Health Organisations (WHOs) global program	311:362	the World Health Organisations (WHOs) global program for "Ending cholera by 2030"	311:391	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	6	2	theme	Global	863:868	arg1	Force					875:879	WHOs Global Task Force	858:879	WHOs Global Task Force on Cholera Control (GTFCC)	858:906	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	2	3	from	cornerstone	296:306	arg1	program					356:362	the World Health Organisations (WHOs) global program	311:362	the World Health Organisations (WHOs) global program for "Ending cholera by 2030"	311:391	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	7	4	theme	vaccine	1049:1055	arg1	development					1027:1037	the development	1023:1037	the development of such a vaccine	1023:1055	We describe here the development of such a vaccine, based on a lyophilized mixture of a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB.					
37085452	9	5	dep	ShancholTM	1547:1556	arg1	OCVs					1571:1574	OCVs	1571:1574	OCVs	1571:1574	It also proved to be well tolerated and to have equivalent oral immunogenicity in mice as ShancholTM and Dukoral® OCVs with regard to both serum and intestinal-mucosal antibody responses.					
37085452	8	6	from	25 °C	1304:1308	arg1	26 months					1291:1299	26 months	1291:1299	26 months at 25 °C	1291:1308	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	1	7	theme	health	175:180	arg1	problem					182:188	an important global health problem	155:188	an important global health problem	155:188	The feared diarrheal disease cholera remains an important global health problem.					
37085452	2	8	theme	endemic	275:281	arg1	cholera					283:289	endemic cholera	275:289	endemic cholera	275:289	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	5	9	theme	present	799:805	arg1	toxin					769:773	the protective cholera toxin B-subunit (CTB)	746:789	the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®	746:817	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	2	10	theme	vaccine	211:217	arg1	cornerstone					296:306	a cornerstone	294:306	a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030"	294:391	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	2	10	theme	vaccine	211:217	arg1	Use					191:193	Use	191:193	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera	191:289	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	1	11	theme	feared	114:119	arg1	cholera					139:145	The feared diarrheal disease cholera	110:145	The feared diarrheal disease cholera	110:145	The feared diarrheal disease cholera remains an important global health problem.					
37085452	6	12	theme	Cholera	884:890	arg1	GTFCC					901:905	GTFCC	901:905	GTFCC	901:905	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	6	12	theme	Cholera	884:890	arg1	Control					892:898	Cholera Control	884:898	Cholera Control (GTFCC)	884:906	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	9	13	theme	antibody	1625:1632	arg1	responses					1634:1642	intestinal-mucosal antibody responses	1606:1642	intestinal-mucosal antibody responses	1606:1642	It also proved to be well tolerated and to have equivalent oral immunogenicity in mice as ShancholTM and Dukoral® OCVs with regard to both serum and intestinal-mucosal antibody responses.					
37085452	8	14	theme	cell	1366:1369	arg1	morphology					1371:1380	cell morphology	1366:1380	cell morphology	1366:1380	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	2	15	theme	global	232:237	arg1	stockpile					239:247	a global stockpile	230:247	a global stockpile	230:247	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	1	16	theme	disease	131:137	arg1	cholera					139:145	The feared diarrheal disease cholera	110:145	The feared diarrheal disease cholera	110:145	The feared diarrheal disease cholera remains an important global health problem.					
37085452	4	17	theme	transport	646:654	arg1	costs					656:660	transport costs	646:660	transport costs	646:660	However, their multicomponent composition and cold-chain requirement increase manufacturing, storage and transport costs.					
37085452	7	18	theme	low-cost	1161:1168	arg1	CTB					1194:1196	a low-cost, recombinantly produced CTB	1159:1196	a low-cost, recombinantly produced CTB	1159:1196	We describe here the development of such a vaccine, based on a lyophilized mixture of a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB.					
37085452	6	19	dep	thermostable	925:936	arg1	dry					939:941	dry	939:941	dry	939:941	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	0	20	theme	B	78:78	arg1	vaccine					101:107	Hikojima whole cell/cholera toxin B subunit oral cholera vaccine	44:107	Hikojima whole cell/cholera toxin B subunit oral cholera vaccine	44:107	A thermostable, dry formulation inactivated Hikojima whole cell/cholera toxin B subunit oral cholera vaccine.					
37085452	1	21	theme	important	158:166	arg1	problem					182:188	an important global health problem	155:188	an important global health problem	155:188	The feared diarrheal disease cholera remains an important global health problem.					
37085452	0	22	theme	oral	88:91	arg1	vaccine					101:107	Hikojima whole cell/cholera toxin B subunit oral cholera vaccine	44:107	Hikojima whole cell/cholera toxin B subunit oral cholera vaccine	44:107	A thermostable, dry formulation inactivated Hikojima whole cell/cholera toxin B subunit oral cholera vaccine.					
37085452	6	23	theme	thermostable	925:936	arg1	vaccine					955:961	a thermostable, dry formulation vaccine	923:961	a thermostable, dry formulation vaccine	923:961	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	6	23	theme	thermostable	925:936	arg1	priority					968:975	a priority	966:975	a priority for further OCV development	966:1003	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	8	24	theme	Ogawa	1413:1417	arg1	loss					1394:1397	no loss	1391:1397	no loss of protective Ogawa and Inaba lipopolysaccharides	1391:1447	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	8	24	theme	Ogawa	1413:1417	arg1	CTB					1452:1454	CTB	1452:1454	CTB	1452:1454	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	5	25	theme	global	711:716	arg1	stockpile					726:734	WHOs global vaccine stockpile	706:734	WHOs global vaccine stockpile	706:734	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	8	26	theme	Inaba	1423:1427	arg1	loss					1394:1397	no loss	1391:1397	no loss of protective Ogawa and Inaba lipopolysaccharides	1391:1447	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	8	26	theme	Inaba	1423:1427	arg1	CTB					1452:1454	CTB	1452:1454	CTB	1452:1454	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	0	27	theme	toxin	72:76	arg1	B					78:78	Hikojima whole cell/cholera toxin B	44:78	Hikojima whole cell/cholera toxin B subunit oral cholera vaccine	44:107	A thermostable, dry formulation inactivated Hikojima whole cell/cholera toxin B subunit oral cholera vaccine.					
37085452	2	28	theme	global	349:354	arg1	program					356:362	the World Health Organisations (WHOs) global program	311:362	the World Health Organisations (WHOs) global program for "Ending cholera by 2030"	311:391	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	7	29	theme	strain	1101:1106	arg1	mixture					1081:1087	a lyophilized mixture	1067:1087	a lyophilized mixture of a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB	1067:1196	We describe here the development of such a vaccine, based on a lyophilized mixture of a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB.					
37085452	3	30	theme	whole-cell	419:428	arg1	ShancholTM					446:455	ShancholTM	446:455	ShancholTM	446:455	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	3	30	theme	whole-cell	419:428	arg1	Euvichol-Plus®					462:475	Euvichol-Plus®	462:475	Euvichol-Plus®	462:475	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	3	30	theme	whole-cell	419:428	arg1	Dukoral®					436:443	Dukoral®	436:443	Dukoral®	436:443	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	3	30	theme	whole-cell	419:428	arg1	OCVs					430:433	Three liquid inactivated whole-cell OCVs	394:433	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®)	394:476	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	2	31	from	stockpile	239:247	arg1	OCV					220:222	OCV	220:222	OCV	220:222	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	2	31	from	stockpile	239:247	arg1	vaccine					211:217	oral cholera vaccine	198:217	oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera	198:289	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	2	31	from	stockpile	239:247	arg1	cornerstone					296:306	a cornerstone	294:306	a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030"	294:391	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	2	31	from	stockpile	239:247	arg1	Use					191:193	Use	191:193	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera	191:289	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	6	32	theme	OCV	989:991	arg1	development					993:1003	further OCV development	981:1003	further OCV development	981:1003	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	2	33	theme	Ending	369:374	arg1	cholera					376:382	"Ending cholera	368:382	"Ending cholera by 2030"	368:391	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	3	34	theme	liquid	400:405	arg1	ShancholTM					446:455	ShancholTM	446:455	ShancholTM	446:455	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	3	34	theme	liquid	400:405	arg1	Euvichol-Plus®					462:475	Euvichol-Plus®	462:475	Euvichol-Plus®	462:475	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	3	34	theme	liquid	400:405	arg1	Dukoral®					436:443	Dukoral®	436:443	Dukoral®	436:443	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	3	34	theme	liquid	400:405	arg1	OCVs					430:433	Three liquid inactivated whole-cell OCVs	394:433	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®)	394:476	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	5	35	theme	protective	750:759	arg1	toxin					769:773	the protective cholera toxin B-subunit (CTB)	746:789	the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®	746:817	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	0	36	theme	cell/cholera	59:70	arg1	B					78:78	Hikojima whole cell/cholera toxin B	44:78	Hikojima whole cell/cholera toxin B subunit oral cholera vaccine	44:107	A thermostable, dry formulation inactivated Hikojima whole cell/cholera toxin B subunit oral cholera vaccine.					
37085452	2	37	theme	World	315:319	arg1	program					356:362	the World Health Organisations (WHOs) global program	311:362	the World Health Organisations (WHOs) global program for "Ending cholera by 2030"	311:391	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	0	38	theme	Hikojima	44:51	arg1	B					78:78	Hikojima whole cell/cholera toxin B	44:78	Hikojima whole cell/cholera toxin B subunit oral cholera vaccine	44:107	A thermostable, dry formulation inactivated Hikojima whole cell/cholera toxin B subunit oral cholera vaccine.					
37085452	5	39	attach	present	799:805	arg1	Dukoral®					810:817	Dukoral®	810:817	Dukoral®	810:817	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	5	39	attach	present	799:805	arg2	toxin					769:773	the protective cholera toxin B-subunit (CTB)	746:789	the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®	746:817	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	2	40	theme	oral	198:201	arg1	OCV					220:222	OCV	220:222	OCV	220:222	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	2	40	theme	oral	198:201	arg1	vaccine					211:217	oral cholera vaccine	198:217	oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera	198:289	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	9	41	theme	equivalent	1505:1514	arg1	immunogenicity					1521:1534	equivalent oral immunogenicity	1505:1534	equivalent oral immunogenicity in mice	1505:1542	It also proved to be well tolerated and to have equivalent oral immunogenicity in mice as ShancholTM and Dukoral® OCVs with regard to both serum and intestinal-mucosal antibody responses.					
37085452	2	42	theme	WHOs	343:346	arg1	program					356:362	the World Health Organisations (WHOs) global program	311:362	the World Health Organisations (WHOs) global program for "Ending cholera by 2030"	311:391	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	0	43	theme	whole	53:57	arg1	B					78:78	Hikojima whole cell/cholera toxin B	44:78	Hikojima whole cell/cholera toxin B subunit oral cholera vaccine	44:107	A thermostable, dry formulation inactivated Hikojima whole cell/cholera toxin B subunit oral cholera vaccine.					
37085452	3	44	dep	OCVs	430:433	arg1	ShancholTM					446:455	ShancholTM	446:455	ShancholTM	446:455	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	3	44	dep	OCVs	430:433	arg1	Euvichol-Plus®					462:475	Euvichol-Plus®	462:475	Euvichol-Plus®	462:475	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	3	44	dep	OCVs	430:433	arg1	Dukoral®					436:443	Dukoral®	436:443	Dukoral®	436:443	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	3	44	dep	OCVs	430:433	arg1	OCVs					430:433	Three liquid inactivated whole-cell OCVs	394:433	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®)	394:476	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	2	45	theme	Organisations	328:340	arg1	program					356:362	the World Health Organisations (WHOs) global program	311:362	the World Health Organisations (WHOs) global program for "Ending cholera by 2030"	311:391	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	6	46	theme	Task	870:873	arg1	Force					875:879	WHOs Global Task Force	858:879	WHOs Global Task Force on Cholera Control (GTFCC)	858:906	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	2	47	from	Use	191:193	arg1	stockpile					239:247	a global stockpile	230:247	a global stockpile	230:247	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	6	48	theme	WHOs	858:861	arg1	Force					875:879	WHOs Global Task Force	858:879	WHOs Global Task Force on Cholera Control (GTFCC)	858:906	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	5	49	theme	B-subunit	775:783	arg1	toxin					769:773	the protective cholera toxin B-subunit (CTB)	746:789	the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®	746:817	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	1	50	theme	global	168:173	arg1	problem					182:188	an important global health problem	155:188	an important global health problem	155:188	The feared diarrheal disease cholera remains an important global health problem.					
37085452	5	51	dep	toxin	769:773	arg1	antigen					791:797	antigen	791:797	the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®	746:817	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	8	52	from	40 °C	1339:1343	arg1	8 months					1327:1334	8 months	1327:1334	8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB	1327:1454	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	5	53	theme	ShancholTM	663:672	arg1	OCVs					693:696	ShancholTM and Euvichol-Plus® OCVs	663:696	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile	663:734	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	5	54	dep	lack	741:744	arg1	results					826:832	results	826:832	results in suboptimal efficacy	826:855	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	5	55	theme	Euvichol-Plus®	678:691	arg1	OCVs					693:696	ShancholTM and Euvichol-Plus® OCVs	663:696	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile	663:734	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	8	56	theme	new	1203:1205	arg1	easy					1225:1228	easy	1225:1228	easy	1225:1228	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	8	56	theme	new	1203:1205	arg1	vaccine					1207:1213	The new vaccine	1199:1213	The new vaccine	1199:1213	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	7	57	theme	Hikojima	1127:1134	arg1	bacteria					1136:1143	formalin-killed Hikojima bacteria	1111:1143	formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB	1111:1196	We describe here the development of such a vaccine, based on a lyophilized mixture of a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB.					
37085452	5	58	from	Dukoral®	810:817	arg1	present					799:805	present	799:805	present	799:805	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	9	59	theme	intestinal-mucosal	1606:1623	arg1	responses					1634:1642	intestinal-mucosal antibody responses	1606:1642	intestinal-mucosal antibody responses	1606:1642	It also proved to be well tolerated and to have equivalent oral immunogenicity in mice as ShancholTM and Dukoral® OCVs with regard to both serum and intestinal-mucosal antibody responses.					
37085452	8	60	theme	morphology	1371:1380	arg1	preservation					1350:1361	preservation	1350:1361	preservation of cell morphology	1350:1380	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	5	61	from	present	799:805	arg1	Dukoral®					810:817	Dukoral®	810:817	Dukoral®	810:817	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	1	62	theme	diarrheal	121:129	arg1	cholera					139:145	The feared diarrheal disease cholera	110:145	The feared diarrheal disease cholera	110:145	The feared diarrheal disease cholera remains an important global health problem.					
37085452	5	63	theme	vaccine	718:724	arg1	stockpile					726:734	WHOs global vaccine stockpile	706:734	WHOs global vaccine stockpile	706:734	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	5	64	theme	suboptimal	837:846	arg1	efficacy					848:855	suboptimal efficacy	837:855	suboptimal efficacy	837:855	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	7	65	theme	bacteria	1136:1143	arg1	strain					1101:1106	a single strain	1092:1106	a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB	1092:1196	We describe here the development of such a vaccine, based on a lyophilized mixture of a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB.					
37085452	0	66	theme	subunit	80:86	arg1	vaccine					101:107	Hikojima whole cell/cholera toxin B subunit oral cholera vaccine	44:107	Hikojima whole cell/cholera toxin B subunit oral cholera vaccine	44:107	A thermostable, dry formulation inactivated Hikojima whole cell/cholera toxin B subunit oral cholera vaccine.					
37085452	6	67	theme	formulation	943:953	arg1	vaccine					955:961	a thermostable, dry formulation vaccine	923:961	a thermostable, dry formulation vaccine	923:961	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	6	67	theme	formulation	943:953	arg1	priority					968:975	a priority	966:975	a priority for further OCV development	966:1003	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	0	68	theme	cholera	93:99	arg1	vaccine					101:107	Hikojima whole cell/cholera toxin B subunit oral cholera vaccine	44:107	Hikojima whole cell/cholera toxin B subunit oral cholera vaccine	44:107	A thermostable, dry formulation inactivated Hikojima whole cell/cholera toxin B subunit oral cholera vaccine.					
37085452	7	69	theme	formalin-killed	1111:1125	arg1	bacteria					1136:1143	formalin-killed Hikojima bacteria	1111:1143	formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB	1111:1196	We describe here the development of such a vaccine, based on a lyophilized mixture of a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB.					
37085452	5	70	theme	WHOs	706:709	arg1	stockpile					726:734	WHOs global vaccine stockpile	706:734	WHOs global vaccine stockpile	706:734	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	9	71	from	immunogenicity	1521:1534	arg1	mice					1539:1542	mice	1539:1542	mice	1539:1542	It also proved to be well tolerated and to have equivalent oral immunogenicity in mice as ShancholTM and Dukoral® OCVs with regard to both serum and intestinal-mucosal antibody responses.					
37085452	0	72	theme	thermostable	2:13	arg1	formulation					20:30	A thermostable, dry formulation	0:30	A thermostable, dry formulation	0:30	A thermostable, dry formulation inactivated Hikojima whole cell/cholera toxin B subunit oral cholera vaccine.					
37085452	0	73	dep	thermostable	2:13	arg1	dry					16:18	dry	16:18	dry	16:18	A thermostable, dry formulation inactivated Hikojima whole cell/cholera toxin B subunit oral cholera vaccine.					
37085452	2	74	theme	2030	387:390	arg1	"					391:391	2030"	387:391	2030"	387:391	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	7	75	theme	lyophilized	1069:1079	arg1	mixture					1081:1087	a lyophilized mixture	1067:1087	a lyophilized mixture of a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB	1067:1196	We describe here the development of such a vaccine, based on a lyophilized mixture of a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB.					
37085452	6	76	theme	further	981:987	arg1	development					993:1003	further OCV development	981:1003	further OCV development	981:1003	WHOs Global Task Force on Cholera Control (GTFCC) has identified a thermostable, dry formulation vaccine as a priority for further OCV development.					
37085452	7	77	dep	low-cost	1161:1168	arg1	produced					1185:1192	produced	1185:1192	produced	1185:1192	We describe here the development of such a vaccine, based on a lyophilized mixture of a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB.					
37085452	3	78	theme	inactivated	407:417	arg1	ShancholTM					446:455	ShancholTM	446:455	ShancholTM	446:455	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	3	78	theme	inactivated	407:417	arg1	Euvichol-Plus®					462:475	Euvichol-Plus®	462:475	Euvichol-Plus®	462:475	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	3	78	theme	inactivated	407:417	arg1	Dukoral®					436:443	Dukoral®	436:443	Dukoral®	436:443	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	3	78	theme	inactivated	407:417	arg1	OCVs					430:433	Three liquid inactivated whole-cell OCVs	394:433	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®)	394:476	Three liquid inactivated whole-cell OCVs (Dukoral®, ShancholTM, and Euvichol-Plus®) are WHO prequalified and have proved to be safe and effective.					
37085452	5	79	theme	cholera	761:767	arg1	toxin					769:773	the protective cholera toxin B-subunit (CTB)	746:789	the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®	746:817	ShancholTM and Euvichol-Plus® OCVs used in WHOs global vaccine stockpile also lack the protective cholera toxin B-subunit (CTB) antigen present in Dukoral®, which results in suboptimal efficacy.					
37085452	2	80	theme	cholera	203:209	arg1	OCV					220:222	OCV	220:222	OCV	220:222	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	2	80	theme	cholera	203:209	arg1	vaccine					211:217	oral cholera vaccine	198:217	oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera	198:289	Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) global program for "Ending cholera by 2030".					
37085452	8	81	with	40 °C	1339:1343	arg1	preservation					1350:1361	preservation	1350:1361	preservation of cell morphology	1350:1380	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	8	81	with	40 °C	1339:1343	arg1	loss					1394:1397	no loss	1391:1397	no loss of protective Ogawa and Inaba lipopolysaccharides	1391:1447	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	8	81	with	40 °C	1339:1343	arg1	CTB					1452:1454	CTB	1452:1454	CTB	1452:1454	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
37085452	4	82	theme	cold-chain	587:596	arg1	requirement					598:608	cold-chain requirement	587:608	cold-chain requirement	587:608	However, their multicomponent composition and cold-chain requirement increase manufacturing, storage and transport costs.					
37085452	9	83	theme	oral	1516:1519	arg1	immunogenicity					1521:1534	equivalent oral immunogenicity	1505:1534	equivalent oral immunogenicity in mice	1505:1542	It also proved to be well tolerated and to have equivalent oral immunogenicity in mice as ShancholTM and Dukoral® OCVs with regard to both serum and intestinal-mucosal antibody responses.					
37085452	4	84	theme	multicomponent	556:569	arg1	composition					571:581	their multicomponent composition	550:581	their multicomponent composition	550:581	However, their multicomponent composition and cold-chain requirement increase manufacturing, storage and transport costs.					
37085452	7	85	theme	single	1094:1099	arg1	strain					1101:1106	a single strain	1092:1106	a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB	1092:1196	We describe here the development of such a vaccine, based on a lyophilized mixture of a single strain of formalin-killed Hikojima bacteria together with a low-cost, recombinantly produced CTB.					
37085452	8	86	dep	Ogawa	1413:1417	arg1	lipopolysaccharides					1429:1447	lipopolysaccharides	1429:1447	lipopolysaccharides	1429:1447	The new vaccine, which is easy and inexpensive to manufacture, could be stored for at least 26 months at 25 °C and for at least 8 months at 40 °C with preservation of cell morphology and with no loss of protective Ogawa and Inaba lipopolysaccharides or CTB.					
36472784	7	0	theme	immunotherapy-based	1420:1438	arg1	treatments					1447:1456	nanomedicine and immunotherapy-based cancer treatments	1403:1456	nanomedicine and immunotherapy-based cancer treatments	1403:1456	This work highlights the promise shown by the HA-Man NCs to target TAMs and thus provides new options for the development of nanomedicine and immunotherapy-based cancer treatments.					
36472784	4	1	theme	TAMs	1015:1018	arg1	infiltration					999:1010	high infiltration	994:1010	high infiltration of TAMs	994:1018	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	6	2	from	accumulation	1233:1244	arg1	tumor					1271:1275	the tumor	1267:1275	the tumor	1267:1275	Finally, the pre-administration of the liposomal liver occupying agent Nanoprimer™ further increased the accumulation of the HA-Man NCs in the tumor.					
36472784	7	3	theme	treatments	1447:1456	arg1	development					1388:1398	the development	1384:1398	the development of nanomedicine and immunotherapy-based cancer treatments	1384:1456	This work highlights the promise shown by the HA-Man NCs to target TAMs and thus provides new options for the development of nanomedicine and immunotherapy-based cancer treatments.					
36472784	3	4	theme	nanocapsules	495:506	arg1	panel					465:469	a panel	463:469	a panel of hyaluronic acid (HA) nanocapsules (NCs)	463:512	This work describes the development and biological evaluation of a panel of hyaluronic acid (HA) nanocapsules (NCs), with different compositions and prepared by different techniques, designed to target macrophages.					
36472784	5	5	theme	non-modified	1107:1118	arg1	NCs					1123:1125	non-modified HA NCs	1107:1125	non-modified HA NCs	1107:1125	These functionalized HA-Man NCs showed a higher accumulation in the tumor compared to non-modified HA NCs.					
36472784	4	6	theme	significant	853:863	arg1	increase					865:872	a significant increase	851:872	a significant increase of NCs uptake by M2 macrophages in vitro	851:913	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	4	7	theme	pro-inflammatory	748:763	arg1	phenotype					765:773	an M1-like pro-inflammatory phenotype	737:773	an M1-like pro-inflammatory phenotype	737:773	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	1	8	theme	cancer	254:259	arg1	prognosis					261:269	cancer prognosis	254:269	cancer prognosis	254:269	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	7	9	theme	new	1368:1370	arg1	options					1372:1378	new options	1368:1378	new options for the development of nanomedicine and immunotherapy-based cancer treatments	1368:1456	This work highlights the promise shown by the HA-Man NCs to target TAMs and thus provides new options for the development of nanomedicine and immunotherapy-based cancer treatments.					
36472784	5	10	theme	higher	1062:1067	arg1	accumulation					1069:1080	a higher accumulation	1060:1080	a higher accumulation in the tumor	1060:1093	These functionalized HA-Man NCs showed a higher accumulation in the tumor compared to non-modified HA NCs.					
36472784	4	11	theme	M1-like	740:746	arg1	phenotype					765:773	an M1-like pro-inflammatory phenotype	737:773	an M1-like pro-inflammatory phenotype	737:773	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	6	12	theme	HA-Man	1253:1258	arg1	NCs					1260:1262	the HA-Man NCs	1249:1262	the HA-Man NCs	1249:1262	Finally, the pre-administration of the liposomal liver occupying agent Nanoprimer™ further increased the accumulation of the HA-Man NCs in the tumor.					
36472784	3	13	theme	different	520:528	arg1	compositions					530:541	different compositions	520:541	different compositions and prepared by different techniques, designed to target macrophages	520:610	This work describes the development and biological evaluation of a panel of hyaluronic acid (HA) nanocapsules (NCs), with different compositions and prepared by different techniques, designed to target macrophages.					
36472784	3	14	theme	acid	485:488	arg1	nanocapsules					495:506	hyaluronic acid (HA) nanocapsules	474:506	hyaluronic acid (HA) nanocapsules (NCs)	474:512	This work describes the development and biological evaluation of a panel of hyaluronic acid (HA) nanocapsules (NCs), with different compositions and prepared by different techniques, designed to target macrophages.					
36472784	3	14	theme	acid	485:488	arg1	NCs					509:511	NCs	509:511	NCs	509:511	This work describes the development and biological evaluation of a panel of hyaluronic acid (HA) nanocapsules (NCs), with different compositions and prepared by different techniques, designed to target macrophages.					
36472784	7	15	theme	cancer	1440:1445	arg1	treatments					1447:1456	nanomedicine and immunotherapy-based cancer treatments	1403:1456	nanomedicine and immunotherapy-based cancer treatments	1403:1456	This work highlights the promise shown by the HA-Man NCs to target TAMs and thus provides new options for the development of nanomedicine and immunotherapy-based cancer treatments.					
36472784	3	16	theme	biological	438:447	arg1	evaluation					449:458	biological evaluation	438:458	biological evaluation	438:458	This work describes the development and biological evaluation of a panel of hyaluronic acid (HA) nanocapsules (NCs), with different compositions and prepared by different techniques, designed to target macrophages.					
36472784	7	17	theme	nanomedicine	1403:1414	arg1	treatments					1447:1456	nanomedicine and immunotherapy-based cancer treatments	1403:1456	nanomedicine and immunotherapy-based cancer treatments	1403:1456	This work highlights the promise shown by the HA-Man NCs to target TAMs and thus provides new options for the development of nanomedicine and immunotherapy-based cancer treatments.					
36472784	4	18	theme	high	994:997	arg1	infiltration					999:1010	high infiltration	994:1010	high infiltration of TAMs	994:1018	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	4	19	with	model	983:987	arg1	infiltration					999:1010	high infiltration	994:1010	high infiltration of TAMs	994:1018	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	1	20	theme	Tumor-associated	97:112	arg1	class					136:140	a class	134:140	a class of immune cells that play a key role in tumor immunosuppression	134:204	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	1	20	theme	Tumor-associated	97:112	arg1	targets					235:241	important targets	225:241	important targets to improve cancer prognosis and treatment	225:283	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	1	20	theme	Tumor-associated	97:112	arg1	TAMs					127:130	TAMs	127:130	TAMs	127:130	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	1	20	theme	Tumor-associated	97:112	arg1	macrophages					114:124	Tumor-associated macrophages	97:124	Tumor-associated macrophages (TAMs)	97:131	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	1	21	theme	key	170:172	arg1	role					174:177	a key role	168:177	a key role	168:177	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	0	22	theme	hyaluronic	17:26	arg1	acid					28:31	Mannose-modified hyaluronic acid	0:31	Mannose-modified hyaluronic acid	0:31	Mannose-modified hyaluronic acid nanocapsules for the targeting of tumor-associated macrophages.					
36472784	4	23	theme	macrophages	717:727	arg1	polarization					686:697	the polarization	682:697	the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype	682:773	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	2	24	theme	special	379:385	arg1	relevance					387:395	special relevance	379:395	special relevance	379:395	Consequently, the engineering of drug delivery nanocarriers that can reach TAMs has acquired special relevance.					
36472784	0	25	theme	Mannose-modified	0:15	arg1	acid					28:31	Mannose-modified hyaluronic acid	0:31	Mannose-modified hyaluronic acid	0:31	Mannose-modified hyaluronic acid nanocapsules for the targeting of tumor-associated macrophages.					
36472784	4	26	theme	M0	702:703	arg1	polarization					686:697	the polarization	682:697	the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype	682:773	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	4	27	theme	M2-like	709:715	arg1	macrophages					717:727	M2-like macrophages	709:727	M2-like macrophages	709:727	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	6	28	theme	liposomal	1167:1175	arg1	liver					1177:1181	the liposomal liver	1163:1181	the liposomal liver occupying agent Nanoprimer™	1163:1209	Finally, the pre-administration of the liposomal liver occupying agent Nanoprimer™ further increased the accumulation of the HA-Man NCs in the tumor.					
36472784	4	29	theme	M2	891:892	arg1	macrophages					894:904	M2 macrophages	891:904	M2 macrophages	891:904	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	6	30	theme	agent	1193:1197	arg1	Nanoprimer™					1199:1209	agent Nanoprimer™	1193:1209	agent Nanoprimer™	1193:1209	Finally, the pre-administration of the liposomal liver occupying agent Nanoprimer™ further increased the accumulation of the HA-Man NCs in the tumor.					
36472784	3	31	theme	panel	465:469	arg1	development					422:432	development	422:432	development	422:432	This work describes the development and biological evaluation of a panel of hyaluronic acid (HA) nanocapsules (NCs), with different compositions and prepared by different techniques, designed to target macrophages.					
36472784	3	31	theme	panel	465:469	arg1	evaluation					449:458	biological evaluation	438:458	biological evaluation	438:458	This work describes the development and biological evaluation of a panel of hyaluronic acid (HA) nanocapsules (NCs), with different compositions and prepared by different techniques, designed to target macrophages.					
36472784	5	32	from	accumulation	1069:1080	arg1	tumor					1089:1093	the tumor	1085:1093	the tumor	1085:1093	These functionalized HA-Man NCs showed a higher accumulation in the tumor compared to non-modified HA NCs.					
36472784	5	33	theme	HA-Man	1042:1047	arg1	NCs					1049:1051	These functionalized HA-Man NCs	1021:1051	These functionalized HA-Man NCs	1021:1051	These functionalized HA-Man NCs showed a higher accumulation in the tumor compared to non-modified HA NCs.					
36472784	3	34	dep	compositions	530:541	arg1	prepared					547:554	prepared	547:554	prepared by different techniques, designed to target macrophages	547:610	This work describes the development and biological evaluation of a panel of hyaluronic acid (HA) nanocapsules (NCs), with different compositions and prepared by different techniques, designed to target macrophages.					
36472784	4	35	theme	fibrosarcoma	964:975	arg1	model					983:987	a MN/MNCA1 fibrosarcoma mouse model	953:987	a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs	953:1018	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	1	36	dep	targets	235:241	arg1	improve					246:252	improve	246:252	to improve cancer prognosis and treatment	243:283	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	3	37	dep	development	422:432	arg1	the					418:420	the	418:420	the	418:420	This work describes the development and biological evaluation of a panel of hyaluronic acid (HA) nanocapsules (NCs), with different compositions and prepared by different techniques, designed to target macrophages.					
36472784	4	38	theme	HA	643:644	arg1	NCs					646:648	plain HA NCs	637:648	plain HA NCs	637:648	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	5	39	theme	HA	1120:1121	arg1	NCs					1123:1125	non-modified HA NCs	1107:1125	non-modified HA NCs	1107:1125	These functionalized HA-Man NCs showed a higher accumulation in the tumor compared to non-modified HA NCs.					
36472784	4	40	theme	MN/MNCA1	955:962	arg1	model					983:987	a MN/MNCA1 fibrosarcoma mouse model	953:987	a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs	953:1018	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	4	41	from	biodistribution	934:948	arg1	model					983:987	a MN/MNCA1 fibrosarcoma mouse model	953:987	a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs	953:1018	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	4	42	theme	improved	925:932	arg1	biodistribution					934:948	an improved biodistribution	922:948	an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs	922:1018	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	3	43	theme	different	559:567	arg1	techniques					569:578	different techniques	559:578	different techniques	559:578	This work describes the development and biological evaluation of a panel of hyaluronic acid (HA) nanocapsules (NCs), with different compositions and prepared by different techniques, designed to target macrophages.					
36472784	4	44	theme	mouse	977:981	arg1	model					983:987	a MN/MNCA1 fibrosarcoma mouse model	953:987	a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs	953:1018	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	4	45	theme	HA	819:820	arg1	functionalization					798:814	the chemical functionalization	785:814	the chemical functionalization of HA with mannose (HA-Man)	785:842	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	2	46	theme	nanocarriers	333:344	arg1	engineering					304:314	the engineering	300:314	the engineering of drug delivery nanocarriers that can reach TAMs	300:364	Consequently, the engineering of drug delivery nanocarriers that can reach TAMs has acquired special relevance.					
36472784	4	47	theme	uptake	881:886	arg1	increase					865:872	a significant increase	851:872	a significant increase of NCs uptake by M2 macrophages in vitro	851:913	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	7	48	theme	HA-Man	1324:1329	arg1	NCs					1331:1333	the HA-Man NCs	1320:1333	the HA-Man NCs	1320:1333	This work highlights the promise shown by the HA-Man NCs to target TAMs and thus provides new options for the development of nanomedicine and immunotherapy-based cancer treatments.					
36472784	3	49	theme	hyaluronic	474:483	arg1	HA					491:492	HA	491:492	HA	491:492	This work describes the development and biological evaluation of a panel of hyaluronic acid (HA) nanocapsules (NCs), with different compositions and prepared by different techniques, designed to target macrophages.					
36472784	3	49	theme	hyaluronic	474:483	arg1	acid					485:488	hyaluronic acid	474:488	hyaluronic acid (HA) nanocapsules (NCs)	474:512	This work describes the development and biological evaluation of a panel of hyaluronic acid (HA) nanocapsules (NCs), with different compositions and prepared by different techniques, designed to target macrophages.					
36472784	5	50	dep	showed	1053:1058	arg1	compared					1095:1102	compared	1095:1102	showed a higher accumulation in the tumor compared to non-modified HA NCs	1053:1125	These functionalized HA-Man NCs showed a higher accumulation in the tumor compared to non-modified HA NCs.					
36472784	4	51	theme	NCs	877:879	arg1	uptake					881:886	NCs uptake	877:886	NCs uptake by M2 macrophages in vitro	877:913	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	6	52	theme	liver	1177:1181	arg1	pre-administration					1141:1158	the pre-administration	1137:1158	the pre-administration of the liposomal liver occupying agent Nanoprimer™	1137:1209	Finally, the pre-administration of the liposomal liver occupying agent Nanoprimer™ further increased the accumulation of the HA-Man NCs in the tumor.					
36472784	2	53	theme	drug	319:322	arg1	nanocarriers					333:344	drug delivery nanocarriers	319:344	drug delivery nanocarriers that can reach TAMs	319:364	Consequently, the engineering of drug delivery nanocarriers that can reach TAMs has acquired special relevance.					
36472784	2	54	theme	delivery	324:331	arg1	nanocarriers					333:344	drug delivery nanocarriers	319:344	drug delivery nanocarriers that can reach TAMs	319:364	Consequently, the engineering of drug delivery nanocarriers that can reach TAMs has acquired special relevance.					
36472784	0	55	theme	macrophages	84:94	arg1	targeting					54:62	the targeting	50:62	the targeting of tumor-associated macrophages	50:94	Mannose-modified hyaluronic acid nanocapsules for the targeting of tumor-associated macrophages.					
36472784	5	56	theme	functionalized	1027:1040	arg1	NCs					1049:1051	These functionalized HA-Man NCs	1021:1051	These functionalized HA-Man NCs	1021:1051	These functionalized HA-Man NCs showed a higher accumulation in the tumor compared to non-modified HA NCs.					
36472784	4	57	theme	chemical	789:796	arg1	functionalization					798:814	the chemical functionalization	785:814	the chemical functionalization of HA with mannose (HA-Man)	785:842	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	1	58	theme	tumor	182:186	arg1	immunosuppression					188:204	tumor immunosuppression	182:204	tumor immunosuppression	182:204	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	1	59	theme	immune	145:150	arg1	cells					152:156	immune cells	145:156	immune cells that play a key role in tumor immunosuppression	145:204	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	4	60	theme	plain	637:641	arg1	NCs					646:648	plain HA NCs	637:648	plain HA NCs	637:648	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	1	61	theme	important	225:233	arg1	targets					235:241	important targets	225:241	important targets to improve cancer prognosis and treatment	225:283	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	1	61	theme	important	225:233	arg1	macrophages					114:124	Tumor-associated macrophages	97:124	Tumor-associated macrophages (TAMs)	97:131	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	0	62	theme	tumor-associated	67:82	arg1	macrophages					84:94	tumor-associated macrophages	67:94	tumor-associated macrophages	67:94	Mannose-modified hyaluronic acid nanocapsules for the targeting of tumor-associated macrophages.					
36472784	1	63	theme	cells	152:156	arg1	class					136:140	a class	134:140	a class of immune cells that play a key role in tumor immunosuppression	134:204	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	1	63	theme	cells	152:156	arg1	macrophages					114:124	Tumor-associated macrophages	97:124	Tumor-associated macrophages (TAMs)	97:131	Tumor-associated macrophages (TAMs), a class of immune cells that play a key role in tumor immunosuppression, are recognized as important targets to improve cancer prognosis and treatment.					
36472784	6	64	theme	NCs	1260:1262	arg1	accumulation					1233:1244	the accumulation	1229:1244	the accumulation of the HA-Man NCs in the tumor	1229:1275	Finally, the pre-administration of the liposomal liver occupying agent Nanoprimer™ further increased the accumulation of the HA-Man NCs in the tumor.					
36472784	4	65	with	functionalization	798:814	arg1	HA-Man					836:841	HA-Man	836:841	HA-Man	836:841	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36472784	4	65	with	functionalization	798:814	arg1	mannose					827:833	mannose	827:833	mannose (HA-Man)	827:842	The results showed that plain HA NCs did not significantly influence the polarization of M0 and M2-like macrophages towards an M1-like pro-inflammatory phenotype; however, the chemical functionalization of HA with mannose (HA-Man) led to a significant increase of NCs uptake by M2 macrophages in vitro and to an improved biodistribution in a MN/MNCA1 fibrosarcoma mouse model with high infiltration of TAMs.					
36217671	2	0	theme	oxygen	547:552	arg1	species					554:560	excessive reactive oxygen species	528:560	excessive reactive oxygen species (ROS)	528:566	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	0	theme	oxygen	547:552	arg1	ROS					563:565	ROS	563:565	ROS	563:565	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	4	1	theme	catalytic	1015:1023	arg1	reactions					1025:1033	cascade catalytic reactions	1007:1033	cascade catalytic reactions	1007:1033	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	4	2	theme	GCNC	895:898	arg1	complex					909:915	the GCNC hydrogel complex	891:915	the GCNC hydrogel complex	891:915	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	2	3	theme	inadequate	397:406	arg1	supply					408:413	inadequate supply	397:413	inadequate supply	397:413	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	5	4	theme	chitosan	1288:1295	arg1	chitosan					1288:1295	chitosan	1288:1295	chitosan	1288:1295	In addition, the byproduct gluconic acid produced by the cascade reaction can activate the amino group of chitosan to reinforce the antibacterial performance and prevent microbial infection.					
36217671	5	4	theme	chitosan	1288:1295	arg1	group					1279:1283	the amino group	1269:1283	the amino group of chitosan to reinforce the antibacterial performance and prevent microbial infection	1269:1370	In addition, the byproduct gluconic acid produced by the cascade reaction can activate the amino group of chitosan to reinforce the antibacterial performance and prevent microbial infection.					
36217671	2	5	theme	reactive	538:545	arg1	species					554:560	excessive reactive oxygen species	528:560	excessive reactive oxygen species (ROS)	528:566	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	5	theme	reactive	538:545	arg1	ROS					563:565	ROS	563:565	ROS	563:565	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	4	6	theme	hydrogel	900:907	arg1	complex					909:915	the GCNC hydrogel complex	891:915	the GCNC hydrogel complex	891:915	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	5	7	theme	cascade	1239:1245	arg1	reaction					1247:1254	the cascade reaction	1235:1254	the cascade reaction	1235:1254	In addition, the byproduct gluconic acid produced by the cascade reaction can activate the amino group of chitosan to reinforce the antibacterial performance and prevent microbial infection.					
36217671	6	8	theme	oxygen	1432:1437	arg1	supply					1439:1444	continuous oxygen supply	1421:1444	continuous oxygen supply	1421:1444	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	4	9	theme	cascade	1007:1013	arg1	reactions					1025:1033	cascade catalytic reactions	1007:1033	cascade catalytic reactions	1007:1033	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	6	10	theme	continuous	1421:1430	arg1	supply					1439:1444	continuous oxygen supply	1421:1444	continuous oxygen supply	1421:1444	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	3	11	theme	wounds	749:754	arg1	microenvironment					720:735	the microenvironment	716:735	the microenvironment of diabetic wounds	716:754	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	2	12	theme	wound	333:337	arg1	healing					339:345	wound healing	333:345	wound healing	333:345	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	1	13	dep	disability	166:175	arg1	The					150:152	The	150:152	The	150:152	The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques.					
36217671	0	14	theme	wounds	142:147	arg1	healing					122:128	healing	122:128	healing of diabetic wounds	122:147	Nanoenzyme-chitosan hydrogel complex with cascade catalytic and self-reinforced antibacterial performance for accelerated healing of diabetic wounds.					
36217671	1	15	theme	wounds	215:220	arg1	disability					166:175	significant disability	154:175	significant disability	154:175	The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques.					
36217671	1	15	theme	wounds	215:220	arg1	rate					190:193	fatality rate	181:193	fatality rate	181:193	The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques.					
36217671	1	16	theme	diabetes	198:205	arg1	wounds					215:220	diabetes chronic wounds	198:220	diabetes chronic wounds	198:220	The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques.					
36217671	0	17	theme	diabetic	133:140	arg1	wounds					142:147	diabetic wounds	133:147	diabetic wounds	133:147	Nanoenzyme-chitosan hydrogel complex with cascade catalytic and self-reinforced antibacterial performance for accelerated healing of diabetic wounds.					
36217671	4	18	theme	GCNE	874:877	arg1	composite					880:888	the GOx-CAT nanoenzyme (GCNE) composite	850:888	the GOx-CAT nanoenzyme (GCNE) composite	850:888	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	4	19	theme	oxygen	1058:1063	arg1	supply					1065:1070	continuous oxygen supply	1047:1070	continuous oxygen supply	1047:1070	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	6	20	theme	byproduct-free	1494:1507	arg1	features					1509:1516	byproduct-free features	1494:1516	byproduct-free features	1494:1516	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	1	21	theme	chronic	207:213	arg1	wounds					215:220	diabetes chronic wounds	198:220	diabetes chronic wounds	198:220	The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques.					
36217671	0	22	theme	hydrogel	20:27	arg1	complex					29:35	Nanoenzyme-chitosan hydrogel complex	0:35	Nanoenzyme-chitosan hydrogel complex with cascade catalytic and self-reinforced antibacterial performance for	0:108	Nanoenzyme-chitosan hydrogel complex with cascade catalytic and self-reinforced antibacterial performance for accelerated healing of diabetic wounds.					
36217671	3	23	theme	diabetic	740:747	arg1	wounds					749:754	diabetic wounds	740:754	diabetic wounds	740:754	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	5	24	theme	gluconic	1209:1216	arg1	acid					1218:1221	the byproduct gluconic acid	1195:1221	the byproduct gluconic acid produced by the cascade reaction	1195:1254	In addition, the byproduct gluconic acid produced by the cascade reaction can activate the amino group of chitosan to reinforce the antibacterial performance and prevent microbial infection.					
36217671	5	25	theme	byproduct	1199:1207	arg1	acid					1218:1221	the byproduct gluconic acid	1195:1221	the byproduct gluconic acid produced by the cascade reaction	1195:1254	In addition, the byproduct gluconic acid produced by the cascade reaction can activate the amino group of chitosan to reinforce the antibacterial performance and prevent microbial infection.					
36217671	2	26	theme	absorption	420:429	arg1	difficulties					431:442	absorption difficulties	420:442	absorption difficulties	420:442	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	26	theme	absorption	420:429	arg1	hyperglycemia					513:525	hyperglycemia	513:525	hyperglycemia	513:525	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	26	theme	absorption	420:429	arg1	species					554:560	excessive reactive oxygen species	528:560	excessive reactive oxygen species (ROS)	528:566	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	26	theme	absorption	420:429	arg1	hypoxia					573:579	hypoxia	573:579	hypoxia	573:579	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	0	27	theme	Nanoenzyme-chitosan	0:18	arg1	complex					29:35	Nanoenzyme-chitosan hydrogel complex	0:35	Nanoenzyme-chitosan hydrogel complex with cascade catalytic and self-reinforced antibacterial performance for	0:108	Nanoenzyme-chitosan hydrogel complex with cascade catalytic and self-reinforced antibacterial performance for accelerated healing of diabetic wounds.					
36217671	3	28	theme	oxygen	779:784	arg1	delivery					786:793	continuous oxygen delivery	768:793	continuous oxygen delivery	768:793	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	4	29	theme	continuous	1047:1056	arg1	supply					1065:1070	continuous oxygen supply	1047:1070	continuous oxygen supply	1047:1070	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	3	30	theme	multifunctional	604:618	arg1	GOx					637:639	GOx	637:639	GOx	637:639	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	3	30	theme	multifunctional	604:618	arg1	oxidase					628:634	a multifunctional glucose oxidase	602:634	a multifunctional glucose oxidase (GOx)	602:640	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	2	31	theme	microenvironment	481:496	arg1	issues					498:503	tanglesome diabetic wound microenvironment issues	455:503	poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia	379:579	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	31	theme	microenvironment	481:496	arg1	hyperglycemia					513:525	hyperglycemia	513:525	hyperglycemia	513:525	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	31	theme	microenvironment	481:496	arg1	species					554:560	excessive reactive oxygen species	528:560	excessive reactive oxygen species (ROS)	528:566	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	31	theme	microenvironment	481:496	arg1	hypoxia					573:579	hypoxia	573:579	hypoxia	573:579	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	4	32	theme	ROS	954:956	arg1	concentrations					965:978	ROS (H2O2) concentrations	954:978	ROS (H2O2) concentrations	954:978	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	6	33	from	damage	1581:1586	arg1	diabetes					1591:1598	diabetes	1591:1598	diabetes	1591:1598	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	4	34	from	glucose	942:948	arg1	wounds					992:997	diabetic wounds	983:997	diabetic wounds	983:997	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	5	35	theme	microbial	1352:1360	arg1	infection					1362:1370	microbial infection	1352:1370	microbial infection	1352:1370	In addition, the byproduct gluconic acid produced by the cascade reaction can activate the amino group of chitosan to reinforce the antibacterial performance and prevent microbial infection.					
36217671	2	36	theme	diabetic	466:473	arg1	issues					498:503	tanglesome diabetic wound microenvironment issues	455:503	poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia	379:579	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	36	theme	diabetic	466:473	arg1	hyperglycemia					513:525	hyperglycemia	513:525	hyperglycemia	513:525	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	36	theme	diabetic	466:473	arg1	species					554:560	excessive reactive oxygen species	528:560	excessive reactive oxygen species (ROS)	528:566	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	36	theme	diabetic	466:473	arg1	hypoxia					573:579	hypoxia	573:579	hypoxia	573:579	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	37	theme	wound	475:479	arg1	issues					498:503	tanglesome diabetic wound microenvironment issues	455:503	poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia	379:579	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	37	theme	wound	475:479	arg1	hyperglycemia					513:525	hyperglycemia	513:525	hyperglycemia	513:525	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	37	theme	wound	475:479	arg1	species					554:560	excessive reactive oxygen species	528:560	excessive reactive oxygen species (ROS)	528:566	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	37	theme	wound	475:479	arg1	hypoxia					573:579	hypoxia	573:579	hypoxia	573:579	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	6	38	theme	GCNC	1394:1397	arg1	complex					1408:1414	This multifunctional GCNC hydrogel complex	1373:1414	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features	1373:1516	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	3	39	theme	nanoenzyme-chitosan	661:679	arg1	complex					697:703	nanoenzyme-chitosan (GCNC) hydrogel complex	661:703	nanoenzyme-chitosan (GCNC) hydrogel complex	661:703	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	4	40	theme	diabetic	1158:1165	arg1	healing					1173:1179	diabetic wound healing	1158:1179	diabetic wound healing	1158:1179	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	2	41	theme	tanglesome	455:464	arg1	issues					498:503	tanglesome diabetic wound microenvironment issues	455:503	poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia	379:579	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	41	theme	tanglesome	455:464	arg1	hyperglycemia					513:525	hyperglycemia	513:525	hyperglycemia	513:525	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	41	theme	tanglesome	455:464	arg1	species					554:560	excessive reactive oxygen species	528:560	excessive reactive oxygen species (ROS)	528:566	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	41	theme	tanglesome	455:464	arg1	hypoxia					573:579	hypoxia	573:579	hypoxia	573:579	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	42	theme	excessive	528:536	arg1	species					554:560	excessive reactive oxygen species	528:560	excessive reactive oxygen species (ROS)	528:566	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	2	42	theme	excessive	528:536	arg1	ROS					563:565	ROS	563:565	ROS	563:565	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	3	43	theme	continuous	768:777	arg1	delivery					786:793	continuous oxygen delivery	768:793	continuous oxygen delivery	768:793	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	4	44	theme	H2O2	959:962	arg1	concentrations					965:978	ROS (H2O2) concentrations	954:978	ROS (H2O2) concentrations	954:978	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	4	45	theme	GOx-CAT	854:860	arg1	composite					880:888	the GOx-CAT nanoenzyme (GCNE) composite	850:888	the GOx-CAT nanoenzyme (GCNE) composite	850:888	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	6	46	theme	extensive	1564:1572	arg1	damage					1581:1586	the extensive tissue damage	1560:1586	the extensive tissue damage in diabetes	1560:1598	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	3	47	theme	wound	809:813	arg1	healing					815:821	efficient wound healing	799:821	efficient wound healing	799:821	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	6	48	theme	multifunctional	1378:1392	arg1	complex					1408:1414	This multifunctional GCNC hydrogel complex	1373:1414	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features	1373:1516	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	5	49	theme	amino	1273:1277	arg1	chitosan					1288:1295	chitosan	1288:1295	chitosan	1288:1295	In addition, the byproduct gluconic acid produced by the cascade reaction can activate the amino group of chitosan to reinforce the antibacterial performance and prevent microbial infection.					
36217671	5	49	theme	amino	1273:1277	arg1	group					1279:1283	the amino group	1269:1283	the amino group of chitosan to reinforce the antibacterial performance and prevent microbial infection	1269:1370	In addition, the byproduct gluconic acid produced by the cascade reaction can activate the amino group of chitosan to reinforce the antibacterial performance and prevent microbial infection.					
36217671	1	50	theme	efficient	254:262	arg1	healing					279:285	efficient diabetic wound healing	254:285	efficient diabetic wound healing techniques	254:296	The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques.					
36217671	0	51	theme	self-reinforced	64:78	arg1	performance					94:104	cascade catalytic and self-reinforced antibacterial performance	42:104	performance	94:104	Nanoenzyme-chitosan hydrogel complex with cascade catalytic and self-reinforced antibacterial performance for accelerated healing of diabetic wounds.					
36217671	4	52	theme	wound	1167:1171	arg1	healing					1173:1179	diabetic wound healing	1158:1179	diabetic wound healing	1158:1179	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	6	53	theme	self-reinforcing	1447:1462	arg1	properties					1478:1487	self-reinforcing antibacterial properties	1447:1487	self-reinforcing antibacterial properties	1447:1487	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	3	54	theme	glucose	620:626	arg1	GOx					637:639	GOx	637:639	GOx	637:639	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	3	54	theme	glucose	620:626	arg1	oxidase					628:634	a multifunctional glucose oxidase	602:634	a multifunctional glucose oxidase (GOx)	602:640	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	0	55	with	complex	29:35	arg1	catalytic					50:58	catalytic	50:58	catalytic	50:58	Nanoenzyme-chitosan hydrogel complex with cascade catalytic and self-reinforced antibacterial performance for accelerated healing of diabetic wounds.					
36217671	0	55	with	complex	29:35	arg1	performance					94:104	cascade catalytic and self-reinforced antibacterial performance	42:104	performance	94:104	Nanoenzyme-chitosan hydrogel complex with cascade catalytic and self-reinforced antibacterial performance for accelerated healing of diabetic wounds.					
36217671	6	56	theme	hydrogel	1399:1406	arg1	complex					1408:1414	This multifunctional GCNC hydrogel complex	1373:1414	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features	1373:1516	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	3	57	theme	hydrogel	688:695	arg1	complex					697:703	nanoenzyme-chitosan (GCNC) hydrogel complex	661:703	nanoenzyme-chitosan (GCNC) hydrogel complex	661:703	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	4	58	theme	nanoenzyme	862:871	arg1	composite					880:888	the GOx-CAT nanoenzyme (GCNE) composite	850:888	the GOx-CAT nanoenzyme (GCNE) composite	850:888	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	2	59	theme	poor	379:382	arg1	penetration					384:394	poor penetration	379:394	poor penetration	379:394	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	4	60	theme	diabetic	983:990	arg1	wounds					992:997	diabetic wounds	983:997	diabetic wounds	983:997	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	3	61	theme	efficient	799:807	arg1	healing					815:821	efficient wound healing	799:821	efficient wound healing	799:821	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	4	62	from	concentrations	965:978	arg1	wounds					992:997	diabetic wounds	983:997	diabetic wounds	983:997	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	2	63	theme	oxygen	311:316	arg1	treatments					318:327	The present oxygen treatments	299:327	The present oxygen treatments for wound healing	299:345	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	6	64	theme	general	1529:1535	arg1	strategy					1537:1544	a general strategy	1527:1544	a general strategy for repairing the extensive tissue damage in diabetes	1527:1598	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	1	65	theme	significant	154:164	arg1	disability					166:175	significant disability	154:175	significant disability	154:175	The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques.					
36217671	5	66	theme	antibacterial	1314:1326	arg1	performance					1328:1338	the antibacterial performance	1310:1338	the antibacterial performance	1310:1338	In addition, the byproduct gluconic acid produced by the cascade reaction can activate the amino group of chitosan to reinforce the antibacterial performance and prevent microbial infection.					
36217671	1	67	theme	diabetic	264:271	arg1	healing					279:285	efficient diabetic wound healing	254:285	efficient diabetic wound healing techniques	254:296	The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques.					
36217671	3	68	theme	GCNC	682:685	arg1	complex					697:703	nanoenzyme-chitosan (GCNC) hydrogel complex	661:703	nanoenzyme-chitosan (GCNC) hydrogel complex	661:703	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	2	69	theme	present	303:309	arg1	treatments					318:327	The present oxygen treatments	299:327	The present oxygen treatments for wound healing	299:345	The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.					
36217671	6	70	theme	tissue	1574:1579	arg1	damage					1581:1586	the extensive tissue damage	1560:1586	the extensive tissue damage in diabetes	1560:1598	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	1	71	theme	wound	273:277	arg1	healing					279:285	efficient diabetic wound healing	254:285	efficient diabetic wound healing techniques	254:296	The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques.					
36217671	6	72	theme	antibacterial	1464:1476	arg1	properties					1478:1487	self-reinforcing antibacterial properties	1447:1487	self-reinforcing antibacterial properties	1447:1487	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	6	73	with	complex	1408:1414	arg1	properties					1478:1487	self-reinforcing antibacterial properties	1447:1487	self-reinforcing antibacterial properties	1447:1487	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	6	73	with	complex	1408:1414	arg1	supply					1439:1444	continuous oxygen supply	1421:1444	continuous oxygen supply	1421:1444	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	6	73	with	complex	1408:1414	arg1	features					1509:1516	byproduct-free features	1494:1516	byproduct-free features	1494:1516	This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.					
36217671	3	74	dep	oxidase	628:634	arg1	complex					697:703	nanoenzyme-chitosan (GCNC) hydrogel complex	661:703	nanoenzyme-chitosan (GCNC) hydrogel complex	661:703	Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing.					
36217671	1	75	theme	healing	279:285	arg1	techniques					287:296	efficient diabetic wound healing techniques	254:296	efficient diabetic wound healing techniques	254:296	The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques.					
36217671	0	76	theme	antibacterial	80:92	arg1	performance					94:104	cascade catalytic and self-reinforced antibacterial performance	42:104	performance	94:104	Nanoenzyme-chitosan hydrogel complex with cascade catalytic and self-reinforced antibacterial performance for accelerated healing of diabetic wounds.					
36217671	1	77	theme	fatality	181:188	arg1	rate					190:193	fatality rate	181:193	fatality rate	181:193	The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques.					
36217671	4	78	theme	cell	1088:1091	arg1	proliferation					1093:1105	cell proliferation	1088:1105	cell proliferation	1088:1105	By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing.					
36217671	1	79	theme	techniques	287:296	arg1	development					239:249	the development	235:249	the development of efficient diabetic wound healing techniques	235:296	The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques.					
37108455	7	0	theme	alveolar-capillary	1252:1269	arg1	barrier					1271:1277	the alveolar-capillary barrier	1248:1277	the alveolar-capillary barrier	1248:1277	VD3 effectively protected the alveolar-capillary barrier against LPS treatment, as indicated by TEER measurement and morphological assessment.					
37108455	6	1	theme	liquid	1164:1169	arg1	proteomics					1210:1219	quantitative liquid chromatography-mass spectrometry-based proteomics	1151:1219	quantitative liquid chromatography-mass spectrometry-based proteomics	1151:1219	The effect of VD3 on the intracellular protein composition in H441 cells was analyzed by quantitative liquid chromatography-mass spectrometry-based proteomics.					
37108455	2	2	dep	mechanisms	299:308	arg1	behind					310:315	behind	310:315	behind this observation	310:332	However, the molecular mechanisms behind this observation are not yet understood.					
37108455	12	3	theme	cell	2085:2088	arg1	barrier					2090:2096	the alveolar-epithelial cell barrier	2061:2096	the alveolar-epithelial cell barrier	2061:2096	DCLK1, as a newly described target molecule for VD3, was prominently stimulated by VD3 (10 nM) and seems to influence the alveolar-epithelial cell barrier and regeneration.					
37108455	0	4	theme	Alveolar-Capillary	0:17	arg1	Protection					27:36	Alveolar-Capillary Barrier Protection	0:36	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.	0:142	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.					
37108455	4	5	theme	epithelial	637:646	arg1	cells					648:652	alveolar epithelial cells	628:652	alveolar epithelial cells	628:652	In this study, we investigated the impact of VD3 on the alveolar-capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types.					
37108455	5	6	theme	proteins	866:873	arg1	expression					797:806	gene expression	792:806	gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1)	792:936	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	6	7	theme	H441	1124:1127	arg1	cells					1129:1133	H441 cells	1124:1133	H441 cells	1124:1133	The effect of VD3 on the intracellular protein composition in H441 cells was analyzed by quantitative liquid chromatography-mass spectrometry-based proteomics.					
37108455	8	8	theme	IL-6	1389:1392	arg1	secretion					1394:1402	the IL-6 secretion	1385:1402	the IL-6 secretion by H441 and OEC	1385:1418	VD3 did not inhibit the IL-6 secretion by H441 and OEC but restricted the diffusion of IL-6 to the epithelial compartment.					
37108455	11	9	theme	matrix	1858:1863	arg1	components					1865:1874	constitutional extracellular matrix components	1829:1874	constitutional extracellular matrix components	1829:1874	Quantitative proteomics identified VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules.					
37108455	5	10	theme	Western	1048:1054	arg1	blot					1056:1059	Western blot	1048:1059	Western blot	1048:1059	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	5	11	theme	cytokines	824:832	arg1	expression					797:806	gene expression	792:806	gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1)	792:936	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	4	12	theme	co-culture	608:617	arg1	model					619:623	a co-culture model	606:623	a co-culture model of alveolar epithelial cells and microvascular endothelial cells	606:688	In this study, we investigated the impact of VD3 on the alveolar-capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types.					
37108455	11	13	theme	immune-regulatory	1914:1930	arg1	molecules					1932:1940	immune-regulatory molecules	1914:1940	immune-regulatory molecules	1914:1940	Quantitative proteomics identified VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules.					
37108455	5	14	theme	surfactant	835:844	arg1	proteins					846:853	surfactant proteins	835:853	surfactant proteins	835:853	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	8	15	theme	epithelial	1464:1473	arg1	compartment					1475:1485	the epithelial compartment	1460:1485	the epithelial compartment	1460:1485	VD3 did not inhibit the IL-6 secretion by H441 and OEC but restricted the diffusion of IL-6 to the epithelial compartment.					
37108455	5	16	theme	doublecortin-like	903:919	arg1	kinase					921:926	doublecortin-like kinase 1	903:928	doublecortin-like kinase 1 (DCLK1)	903:936	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	5	16	theme	doublecortin-like	903:919	arg1	DCLK1					931:935	DCLK1	931:935	DCLK1	931:935	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	11	17	theme	abundance	1798:1806	arg1	changes					1808:1814	VD3-dependent protein abundance changes	1776:1814	VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules	1776:1940	Quantitative proteomics identified VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules.					
37108455	6	18	theme	spectrometry-based	1191:1208	arg1	proteomics					1210:1219	quantitative liquid chromatography-mass spectrometry-based proteomics	1151:1219	quantitative liquid chromatography-mass spectrometry-based proteomics	1151:1219	The effect of VD3 on the intracellular protein composition in H441 cells was analyzed by quantitative liquid chromatography-mass spectrometry-based proteomics.					
37108455	11	19	theme	VD3-dependent	1776:1788	arg1	changes					1808:1814	VD3-dependent protein abundance changes	1776:1814	VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules	1776:1940	Quantitative proteomics identified VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules.					
37108455	0	20	theme	Type-Specific	58:70	arg1	Effects					72:78	Lung Cell Type-Specific Effects	48:78	Lung Cell Type-Specific Effects	48:78	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.					
37108455	9	21	theme	protein	1545:1551	arg1	expression					1555:1564	the surfactant protein A expression	1530:1564	the surfactant protein A expression induced in the co-culture system by LPS treatment	1530:1614	Further, VD3 could significantly suppress the surfactant protein A expression induced in the co-culture system by LPS treatment.					
37108455	4	22	theme	endothelial	672:682	arg1	cells					684:688	microvascular endothelial cells	658:688	microvascular endothelial cells	658:688	In this study, we investigated the impact of VD3 on the alveolar-capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types.					
37108455	11	23	theme	constitutional	1829:1842	arg1	components					1865:1874	constitutional extracellular matrix components	1829:1874	constitutional extracellular matrix components	1829:1874	Quantitative proteomics identified VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules.					
37108455	5	24	theme	bacterial	756:764	arg1	lipopolysaccharide					771:788	lipopolysaccharide	771:788	lipopolysaccharide	771:788	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	5	24	theme	bacterial	756:764	arg1	LPS					766:768	bacterial LPS	756:768	bacterial LPS (lipopolysaccharide)	756:789	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	4	25	from	impact	555:560	arg1	barrier					595:601	the alveolar-capillary barrier	572:601	the alveolar-capillary barrier	572:601	In this study, we investigated the impact of VD3 on the alveolar-capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types.					
37108455	3	26	theme	alveolar	399:406	arg1	differentiation					421:435	alveolar type II cell differentiation	399:435	alveolar type II cell differentiation	399:435	VD3 is known to stimulate lung maturity, alveolar type II cell differentiation, or pulmonary surfactant synthesis and guides epithelial defense during infection.					
37108455	9	27	theme	co-culture	1581:1590	arg1	system					1592:1597	the co-culture system	1577:1597	the co-culture system	1577:1597	Further, VD3 could significantly suppress the surfactant protein A expression induced in the co-culture system by LPS treatment.					
37108455	9	28	theme	A	1553:1553	arg1	expression					1555:1564	the surfactant protein A expression	1530:1564	the surfactant protein A expression induced in the co-culture system by LPS treatment	1530:1614	Further, VD3 could significantly suppress the surfactant protein A expression induced in the co-culture system by LPS treatment.					
37108455	4	29	theme	cell	721:724	arg1	types					726:730	the individual cell types	706:730	the individual cell types	706:730	In this study, we investigated the impact of VD3 on the alveolar-capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types.					
37108455	10	30	theme	LL-37	1670:1674	arg1	levels					1634:1639	high levels	1629:1639	high levels	1629:1639	VD3 induced high levels of the antimicrobial peptide LL-37, which counteracted effects by LPS and strengthened the barrier.					
37108455	5	31	theme	gene	792:795	arg1	expression					797:806	gene expression	792:806	gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1)	792:936	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	1	32	theme	Low	144:146	arg1	levels					154:159	Low serum levels	144:159	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3)	144:195	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS.					
37108455	5	33	theme	proteins	846:853	arg1	expression					797:806	gene expression	792:806	gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1)	792:936	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	12	34	theme	described	1961:1969	arg1	molecule					1978:1985	a newly described target molecule	1953:1985	a newly described target molecule for VD3	1953:1993	DCLK1, as a newly described target molecule for VD3, was prominently stimulated by VD3 (10 nM) and seems to influence the alveolar-epithelial cell barrier and regeneration.					
37108455	7	35	theme	TEER	1318:1321	arg1	measurement					1323:1333	TEER measurement	1318:1333	TEER measurement	1318:1333	VD3 effectively protected the alveolar-capillary barrier against LPS treatment, as indicated by TEER measurement and morphological assessment.					
37108455	3	36	theme	surfactant	451:460	arg1	synthesis					462:470	pulmonary surfactant synthesis	441:470	pulmonary surfactant synthesis	441:470	VD3 is known to stimulate lung maturity, alveolar type II cell differentiation, or pulmonary surfactant synthesis and guides epithelial defense during infection.					
37108455	6	37	theme	protein	1101:1107	arg1	composition					1109:1119	the intracellular protein composition	1083:1119	the intracellular protein composition in H441 cells	1083:1133	The effect of VD3 on the intracellular protein composition in H441 cells was analyzed by quantitative liquid chromatography-mass spectrometry-based proteomics.					
37108455	5	38	with	stimulation	739:749	arg1	lipopolysaccharide					771:788	lipopolysaccharide	771:788	lipopolysaccharide	771:788	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	5	38	with	stimulation	739:749	arg1	LPS					766:768	bacterial LPS	756:768	bacterial LPS (lipopolysaccharide)	756:789	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	0	39	dep	Protection	27:36	arg1	Effects					72:78	Lung Cell Type-Specific Effects	48:78	Lung Cell Type-Specific Effects	48:78	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.					
37108455	0	39	dep	Protection	27:36	arg1	Mechanisms					94:103	Molecular Mechanisms	84:103	Molecular Mechanisms	84:103	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.					
37108455	5	40	theme	antimicrobial	876:888	arg1	peptide					890:896	antimicrobial peptide	876:896	antimicrobial peptide	876:896	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	4	41	theme	VD3	565:567	arg1	impact					555:560	the impact	551:560	the impact of VD3 on the alveolar-capillary barrier	551:601	In this study, we investigated the impact of VD3 on the alveolar-capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types.					
37108455	11	42	theme	Quantitative	1741:1752	arg1	proteomics					1754:1763	Quantitative proteomics	1741:1763	Quantitative proteomics	1741:1763	Quantitative proteomics identified VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules.					
37108455	8	43	theme	IL-6	1452:1455	arg1	diffusion					1439:1447	the diffusion	1435:1447	the diffusion of IL-6 to the epithelial compartment	1435:1485	VD3 did not inhibit the IL-6 secretion by H441 and OEC but restricted the diffusion of IL-6 to the epithelial compartment.					
37108455	6	44	from	effect	1066:1071	arg1	composition					1109:1119	the intracellular protein composition	1083:1119	the intracellular protein composition in H441 cells	1083:1133	The effect of VD3 on the intracellular protein composition in H441 cells was analyzed by quantitative liquid chromatography-mass spectrometry-based proteomics.					
37108455	3	45	theme	epithelial	483:492	arg1	defense					494:500	epithelial defense	483:500	epithelial defense during infection	483:517	VD3 is known to stimulate lung maturity, alveolar type II cell differentiation, or pulmonary surfactant synthesis and guides epithelial defense during infection.					
37108455	6	46	theme	VD3	1076:1078	arg1	effect					1066:1071	The effect	1062:1071	The effect of VD3 on the intracellular protein composition in H441 cells	1062:1133	The effect of VD3 on the intracellular protein composition in H441 cells was analyzed by quantitative liquid chromatography-mass spectrometry-based proteomics.					
37108455	1	47	theme	trauma	239:244	arg1	patients					246:253	trauma patients	239:253	trauma patients with sepsis or ARDS	239:273	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS.					
37108455	6	48	theme	quantitative	1151:1162	arg1	proteomics					1210:1219	quantitative liquid chromatography-mass spectrometry-based proteomics	1151:1219	quantitative liquid chromatography-mass spectrometry-based proteomics	1151:1219	The effect of VD3 on the intracellular protein composition in H441 cells was analyzed by quantitative liquid chromatography-mass spectrometry-based proteomics.					
37108455	5	49	theme	transport	856:864	arg1	proteins					866:873	transport proteins	856:873	transport proteins	856:873	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	1	50	theme	1α	164:165	arg1	levels					154:159	Low serum levels	144:159	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3)	144:195	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS.					
37108455	12	51	theme	alveolar-epithelial	2065:2083	arg1	barrier					2090:2096	the alveolar-epithelial cell barrier	2061:2096	the alveolar-epithelial cell barrier	2061:2096	DCLK1, as a newly described target molecule for VD3, was prominently stimulated by VD3 (10 nM) and seems to influence the alveolar-epithelial cell barrier and regeneration.					
37108455	10	52	theme	high	1629:1632	arg1	levels					1634:1639	high levels	1629:1639	high levels	1629:1639	VD3 induced high levels of the antimicrobial peptide LL-37, which counteracted effects by LPS and strengthened the barrier.					
37108455	1	53	theme	25-dihydroxyvitamin	168:186	arg1	VD3					192:194	VD3	192:194	VD3	192:194	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS.					
37108455	1	53	theme	25-dihydroxyvitamin	168:186	arg1	1α					164:165	1α	164:165	1α	164:165	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS.					
37108455	1	53	theme	25-dihydroxyvitamin	168:186	arg1	D3					188:189	25-dihydroxyvitamin D3	168:189	25-dihydroxyvitamin D3 (VD3)	168:195	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS.					
37108455	4	54	theme	alveolar	628:635	arg1	cells					648:652	alveolar epithelial cells	628:652	alveolar epithelial cells	628:652	In this study, we investigated the impact of VD3 on the alveolar-capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types.					
37108455	11	55	theme	surfactant-associated	1880:1900	arg1	proteins					1902:1909	surfactant-associated proteins	1880:1909	surfactant-associated proteins	1880:1909	Quantitative proteomics identified VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules.					
37108455	0	56	theme	25-Dihydroxyvitamin	120:138	arg1	D3					140:141	25-Dihydroxyvitamin D3	120:141	25-Dihydroxyvitamin D3	120:141	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.					
37108455	0	56	theme	25-Dihydroxyvitamin	120:138	arg1	1α					116:117	1α	116:117	1α	116:117	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.					
37108455	5	57	theme	inflammatory	811:822	arg1	cytokines					824:832	inflammatory cytokines	811:832	inflammatory cytokines	811:832	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	10	58	theme	antimicrobial	1648:1660	arg1	LL-37					1670:1674	the antimicrobial peptide LL-37	1644:1674	the antimicrobial peptide LL-37	1644:1674	VD3 induced high levels of the antimicrobial peptide LL-37, which counteracted effects by LPS and strengthened the barrier.					
37108455	1	59	from	mortality	226:234	arg1	patients					246:253	trauma patients	239:253	trauma patients with sepsis or ARDS	239:273	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS.					
37108455	4	60	theme	cells	684:688	arg1	model					619:623	a co-culture model	606:623	a co-culture model of alveolar epithelial cells and microvascular endothelial cells	606:688	In this study, we investigated the impact of VD3 on the alveolar-capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types.					
37108455	7	61	theme	LPS	1287:1289	arg1	treatment					1291:1299	LPS treatment	1287:1299	LPS treatment	1287:1299	VD3 effectively protected the alveolar-capillary barrier against LPS treatment, as indicated by TEER measurement and morphological assessment.					
37108455	5	62	theme	kinase	921:926	arg1	expression					797:806	gene expression	792:806	gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1)	792:936	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	9	63	theme	surfactant	1534:1543	arg1	expression					1555:1564	the surfactant protein A expression	1530:1564	the surfactant protein A expression induced in the co-culture system by LPS treatment	1530:1614	Further, VD3 could significantly suppress the surfactant protein A expression induced in the co-culture system by LPS treatment.					
37108455	6	64	theme	chromatography-mass	1171:1189	arg1	proteomics					1210:1219	quantitative liquid chromatography-mass spectrometry-based proteomics	1151:1219	quantitative liquid chromatography-mass spectrometry-based proteomics	1151:1219	The effect of VD3 on the intracellular protein composition in H441 cells was analyzed by quantitative liquid chromatography-mass spectrometry-based proteomics.					
37108455	4	65	theme	cells	648:652	arg1	model					619:623	a co-culture model	606:623	a co-culture model of alveolar epithelial cells and microvascular endothelial cells	606:688	In this study, we investigated the impact of VD3 on the alveolar-capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types.					
37108455	11	66	theme	protein	1790:1796	arg1	changes					1808:1814	VD3-dependent protein abundance changes	1776:1814	VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules	1776:1940	Quantitative proteomics identified VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules.					
37108455	0	67	theme	Molecular	84:92	arg1	Mechanisms					94:103	Molecular Mechanisms	84:103	Molecular Mechanisms	84:103	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.					
37108455	5	68	theme	peptide	890:896	arg1	expression					797:806	gene expression	792:806	gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1)	792:936	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	4	69	theme	microvascular	658:670	arg1	cells					684:688	microvascular endothelial cells	658:688	microvascular endothelial cells	658:688	In this study, we investigated the impact of VD3 on the alveolar-capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types.					
37108455	11	70	theme	extracellular	1844:1856	arg1	components					1865:1874	constitutional extracellular matrix components	1829:1874	constitutional extracellular matrix components	1829:1874	Quantitative proteomics identified VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules.					
37108455	2	71	theme	molecular	289:297	arg1	mechanisms					299:308	the molecular mechanisms	285:308	the molecular mechanisms behind this observation	285:332	However, the molecular mechanisms behind this observation are not yet understood.					
37108455	5	72	theme	real-time	955:963	arg1	PCR					965:967	real-time PCR	955:967	real-time PCR	955:967	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	3	73	theme	type	408:411	arg1	differentiation					421:435	alveolar type II cell differentiation	399:435	alveolar type II cell differentiation	399:435	VD3 is known to stimulate lung maturity, alveolar type II cell differentiation, or pulmonary surfactant synthesis and guides epithelial defense during infection.					
37108455	0	74	theme	Barrier	19:25	arg1	Protection					27:36	Alveolar-Capillary Barrier Protection	0:36	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.	0:142	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.					
37108455	7	75	theme	morphological	1339:1351	arg1	assessment					1353:1362	morphological assessment	1339:1362	morphological assessment	1339:1362	VD3 effectively protected the alveolar-capillary barrier against LPS treatment, as indicated by TEER measurement and morphological assessment.					
37108455	4	76	theme	individual	710:719	arg1	types					726:730	the individual cell types	706:730	the individual cell types	706:730	In this study, we investigated the impact of VD3 on the alveolar-capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types.					
37108455	10	77	theme	peptide	1662:1668	arg1	LL-37					1670:1674	the antimicrobial peptide LL-37	1644:1674	the antimicrobial peptide LL-37	1644:1674	VD3 induced high levels of the antimicrobial peptide LL-37, which counteracted effects by LPS and strengthened the barrier.					
37108455	0	78	theme	In	38:39	arg1	Protection					27:36	Alveolar-Capillary Barrier Protection	0:36	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.	0:142	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.					
37108455	9	79	theme	LPS	1602:1604	arg1	treatment					1606:1614	LPS treatment	1602:1614	LPS treatment	1602:1614	Further, VD3 could significantly suppress the surfactant protein A expression induced in the co-culture system by LPS treatment.					
37108455	12	80	theme	target	1971:1976	arg1	molecule					1978:1985	a newly described target molecule	1953:1985	a newly described target molecule for VD3	1953:1993	DCLK1, as a newly described target molecule for VD3, was prominently stimulated by VD3 (10 nM) and seems to influence the alveolar-epithelial cell barrier and regeneration.					
37108455	3	81	theme	lung	384:387	arg1	maturity					389:396	lung maturity	384:396	lung maturity	384:396	VD3 is known to stimulate lung maturity, alveolar type II cell differentiation, or pulmonary surfactant synthesis and guides epithelial defense during infection.					
37108455	3	82	theme	cell	416:419	arg1	differentiation					421:435	alveolar type II cell differentiation	399:435	alveolar type II cell differentiation	399:435	VD3 is known to stimulate lung maturity, alveolar type II cell differentiation, or pulmonary surfactant synthesis and guides epithelial defense during infection.					
37108455	1	83	with	patients	246:253	arg1	ARDS					270:273	ARDS	270:273	ARDS	270:273	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS.					
37108455	1	83	with	patients	246:253	arg1	sepsis					260:265	sepsis	260:265	sepsis	260:265	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS.					
37108455	1	84	theme	serum	148:152	arg1	levels					154:159	Low serum levels	144:159	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3)	144:195	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS.					
37108455	6	85	from	composition	1109:1119	arg1	cells					1129:1133	H441 cells	1124:1133	H441 cells	1124:1133	The effect of VD3 on the intracellular protein composition in H441 cells was analyzed by quantitative liquid chromatography-mass spectrometry-based proteomics.					
37108455	0	86	dep	In	38:39	arg1	Vitro					41:45	Vitro	41:45	Vitro	41:45	Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.					
37108455	6	87	theme	intracellular	1087:1099	arg1	composition					1109:1119	the intracellular protein composition	1083:1119	the intracellular protein composition in H441 cells	1083:1133	The effect of VD3 on the intracellular protein composition in H441 cells was analyzed by quantitative liquid chromatography-mass spectrometry-based proteomics.					
37108455	3	88	theme	pulmonary	441:449	arg1	synthesis					462:470	pulmonary surfactant synthesis	441:470	pulmonary surfactant synthesis	441:470	VD3 is known to stimulate lung maturity, alveolar type II cell differentiation, or pulmonary surfactant synthesis and guides epithelial defense during infection.					
37108455	5	89	theme	corresponding	976:988	arg1	proteins					990:997	corresponding proteins	976:997	corresponding proteins	976:997	After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot.					
37108455	4	90	theme	alveolar-capillary	576:593	arg1	barrier					595:601	the alveolar-capillary barrier	572:601	the alveolar-capillary barrier	572:601	In this study, we investigated the impact of VD3 on the alveolar-capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types.					
37108455	1	91	theme	higher	219:224	arg1	mortality					226:234	a higher mortality	217:234	a higher mortality in trauma patients with sepsis or ARDS	217:273	Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS.					
37028725	3	0	theme	various	616:622	arg1	analyses					624:631	various analyses	616:631	various analyses	616:631	First, Fe3O4@GA nanocomposite was synthesized by the co-precipitation approach and then its morphology, elemental composition, thermal stability, and functional groups were characterized by various analyses.					
37028725	3	1	theme	Fe3O4	433:437	arg1	nanocomposite					442:454	Fe3O4@GA nanocomposite	433:454	Fe3O4@GA nanocomposite	433:454	First, Fe3O4@GA nanocomposite was synthesized by the co-precipitation approach and then its morphology, elemental composition, thermal stability, and functional groups were characterized by various analyses.					
37028725	11	2	theme	casting	1433:1439	arg1	solution					1441:1448	the membrane casting solution	1420:1448	the membrane casting solution	1420:1448	According to the results, adding Fe3O4@GA green nanocomposite to the membrane casting solution is a suitable and efficient procedure for modifying NF membranes.					
37028725	10	3	theme	Reactive	1247:1254	arg1	Red-195					1256:1262	98% Reactive Red-195	1243:1262	98% Reactive Red-195	1243:1262	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	1	4	theme	hydrophobic	257:267	arg1	nature					269:274	their hydrophobic nature	251:274	their hydrophobic nature	251:274	Nanofiltration (NF) membranes are promising media for water and wastewater treatment; however, they suffer from their hydrophobic nature and low permeability.					
37028725	10	5	theme	Blue-19	1278:1284	arg1	rates					1234:1238	high rejection rates	1219:1238	high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic	1219:1315	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	2	6	mod	modified	360:367	arg1	membrane					347:354	the polyvinyl chloride (PVC) NF membrane	315:354	the polyvinyl chloride (PVC) NF membrane	315:354	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	2	6	mod	modified	360:367	arg3	nanocomposite					411:423	iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite	372:423	iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite	372:423	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	1	7	theme	promising	173:181	arg1	media					183:187	promising media	173:187	promising media for water and wastewater treatment	173:222	Nanofiltration (NF) membranes are promising media for water and wastewater treatment; however, they suffer from their hydrophobic nature and low permeability.					
37028725	1	7	theme	promising	173:181	arg1	membranes					159:167	Nanofiltration (NF) membranes	139:167	Nanofiltration (NF) membranes	139:167	Nanofiltration (NF) membranes are promising media for water and wastewater treatment; however, they suffer from their hydrophobic nature and low permeability.					
37028725	10	8	theme	%	1267:1267	arg1	Blue-19					1278:1284	95% Reactive Blue-19	1265:1284	95% Reactive Blue-19	1265:1284	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	0	9	theme	organic	119:125	arg1	pollutants					127:136	organic pollutants	119:136	organic pollutants	119:136	Fe3O4@Gum Arabic modified polyvinyl chloride membranes to improve antifouling performance and separation efficiency of organic pollutants.					
37028725	3	10	theme	co-precipitation	479:494	arg1	approach					496:503	the co-precipitation approach	475:503	the co-precipitation approach	475:503	First, Fe3O4@GA nanocomposite was synthesized by the co-precipitation approach and then its morphology, elemental composition, thermal stability, and functional groups were characterized by various analyses.					
37028725	6	11	theme	mechanical	887:896	arg1	strength					898:905	mechanical strength	887:905	mechanical strength	887:905	The characteristics of fabricated membranes were assessed by mechanical strength, water contact angle, pore size, and porosity measurements.					
37028725	7	12	theme	@	984:984	arg1	membrane					992:999	The optimum Fe3O4@GA/PVC membrane	967:999	The optimum Fe3O4@GA/PVC membrane	967:999	The optimum Fe3O4@GA/PVC membrane had a 52 L m-2.					
37028725	11	13	theme	suitable	1455:1462	arg1	procedure					1478:1486	a suitable and efficient procedure	1453:1486	a suitable and efficient procedure for modifying NF membranes	1453:1513	According to the results, adding Fe3O4@GA green nanocomposite to the membrane casting solution is a suitable and efficient procedure for modifying NF membranes.					
37028725	7	14	theme	optimum	971:977	arg1	membrane					992:999	The optimum Fe3O4@GA/PVC membrane	967:999	The optimum Fe3O4@GA/PVC membrane	967:999	The optimum Fe3O4@GA/PVC membrane had a 52 L m-2.					
37028725	6	15	theme	membranes	860:868	arg1	characteristics					830:844	The characteristics	826:844	The characteristics of fabricated membranes	826:868	The characteristics of fabricated membranes were assessed by mechanical strength, water contact angle, pore size, and porosity measurements.					
37028725	7	16	contain	had	1001:1003	arg2	52 L m-2					1007:1014	a 52 L m-2	1005:1014	a 52 L m-2	1005:1014	The optimum Fe3O4@GA/PVC membrane had a 52 L m-2.					
37028725	7	16	contain	had	1001:1003	arg1	membrane					992:999	The optimum Fe3O4@GA/PVC membrane	967:999	The optimum Fe3O4@GA/PVC membrane	967:999	The optimum Fe3O4@GA/PVC membrane had a 52 L m-2.					
37028725	3	17	theme	thermal	553:559	arg1	stability					561:569	thermal stability	553:569	thermal stability	553:569	First, Fe3O4@GA nanocomposite was synthesized by the co-precipitation approach and then its morphology, elemental composition, thermal stability, and functional groups were characterized by various analyses.					
37028725	1	18	theme	Nanofiltration	139:152	arg1	media					183:187	promising media	173:187	promising media for water and wastewater treatment	173:222	Nanofiltration (NF) membranes are promising media for water and wastewater treatment; however, they suffer from their hydrophobic nature and low permeability.					
37028725	1	18	theme	Nanofiltration	139:152	arg1	membranes					159:167	Nanofiltration (NF) membranes	139:167	Nanofiltration (NF) membranes	139:167	Nanofiltration (NF) membranes are promising media for water and wastewater treatment; however, they suffer from their hydrophobic nature and low permeability.					
37028725	10	19	theme	%	1293:1293	arg1	Rifampicin					1295:1304	96% Rifampicin antibiotic	1291:1315	96% Rifampicin antibiotic	1291:1315	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	0	20	theme	antifouling	66:76	arg1	performance					78:88	antifouling performance	66:88	antifouling performance	66:88	Fe3O4@Gum Arabic modified polyvinyl chloride membranes to improve antifouling performance and separation efficiency of organic pollutants.					
37028725	6	21	theme	contact	914:920	arg1	angle					922:926	water contact angle	908:926	water contact angle	908:926	The characteristics of fabricated membranes were assessed by mechanical strength, water contact angle, pore size, and porosity measurements.					
37028725	10	22	theme	0.25 wt	1320:1326	arg1	membrane					1345:1352	Fe3O4@GA/PVC membrane	1332:1352	Fe3O4@GA/PVC membrane	1332:1352	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	10	22	theme	0.25 wt	1320:1326	arg1	%					1327:1327	0.25 wt%	1320:1327	0.25 wt% of Fe3O4@GA/PVC membrane	1320:1352	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	10	23	theme	GA/PVC	1147:1152	arg1	membrane					1154:1161	the Fe3O4@GA/PVC membrane	1137:1161	the Fe3O4@GA/PVC membrane	1137:1161	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	2	24	theme	Arabic	393:398	arg1	nanocomposite					411:423	iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite	372:423	iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite	372:423	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	10	25	theme	antibiotic	1306:1315	arg1	Rifampicin					1295:1304	96% Rifampicin antibiotic	1291:1315	96% Rifampicin antibiotic	1291:1315	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	10	26	theme	Fe3O4	1141:1145	arg1	membrane					1154:1161	the Fe3O4@GA/PVC membrane	1137:1161	the Fe3O4@GA/PVC membrane	1137:1161	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	0	27	theme	Fe3O4	0:4	arg1	Arabic					10:15	Fe3O4@Gum Arabic	0:15	Fe3O4@Gum Arabic	0:15	Fe3O4@Gum Arabic modified polyvinyl chloride membranes to improve antifouling performance and separation efficiency of organic pollutants.					
37028725	2	28	theme	@	388:388	arg1	Arabic					393:398	iron (III) oxide@Gum Arabic	372:398	iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite	372:423	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	2	28	theme	@	388:388	arg1	GA					407:408	Fe3O4@GA	401:408	Fe3O4@GA	401:408	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	10	29	theme	@	1337:1337	arg1	membrane					1345:1352	Fe3O4@GA/PVC membrane	1332:1352	Fe3O4@GA/PVC membrane	1332:1352	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	0	30	theme	Gum	6:8	arg1	Arabic					10:15	Fe3O4@Gum Arabic	0:15	Fe3O4@Gum Arabic	0:15	Fe3O4@Gum Arabic modified polyvinyl chloride membranes to improve antifouling performance and separation efficiency of organic pollutants.					
37028725	1	31	theme	low	280:282	arg1	permeability					284:295	low permeability	280:295	low permeability	280:295	Nanofiltration (NF) membranes are promising media for water and wastewater treatment; however, they suffer from their hydrophobic nature and low permeability.					
37028725	3	32	theme	functional	576:585	arg1	groups					587:592	functional groups	576:592	functional groups	576:592	First, Fe3O4@GA nanocomposite was synthesized by the co-precipitation approach and then its morphology, elemental composition, thermal stability, and functional groups were characterized by various analyses.					
37028725	9	33	theme	water	1028:1032	arg1	flux					1034:1037	bar-1 water flux	1022:1037	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).	1022:1088	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).					
37028725	2	34	theme	Fe3O4	401:405	arg1	Arabic					393:398	iron (III) oxide@Gum Arabic	372:398	iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite	372:423	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	2	34	theme	Fe3O4	401:405	arg1	GA					407:408	Fe3O4@GA	401:408	Fe3O4@GA	401:408	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	2	35	theme	chloride	329:336	arg1	membrane					347:354	the polyvinyl chloride (PVC) NF membrane	315:354	the polyvinyl chloride (PVC) NF membrane	315:354	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	0	36	theme	chloride	36:43	arg1	membranes					45:53	polyvinyl chloride membranes	26:53	polyvinyl chloride membranes	26:53	Fe3O4@Gum Arabic modified polyvinyl chloride membranes to improve antifouling performance and separation efficiency of organic pollutants.					
37028725	10	37	theme	membrane	1345:1352	arg1	membrane					1345:1352	Fe3O4@GA/PVC membrane	1332:1352	Fe3O4@GA/PVC membrane	1332:1352	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	10	37	theme	membrane	1345:1352	arg1	%					1327:1327	0.25 wt%	1320:1327	0.25 wt% of Fe3O4@GA/PVC membrane	1320:1352	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	11	38	theme	@	1393:1393	arg1	nanocomposite					1403:1415	Fe3O4@GA green nanocomposite	1388:1415	Fe3O4@GA green nanocomposite	1388:1415	According to the results, adding Fe3O4@GA green nanocomposite to the membrane casting solution is a suitable and efficient procedure for modifying NF membranes.					
37028725	4	39	theme	membrane	712:719	arg1	solution					692:699	the casting solution	680:699	the casting solution of the PVC membrane	680:719	Next, the prepared nanocomposite was added to the casting solution of the PVC membrane.					
37028725	9	40	theme	high	1046:1049	arg1	FRR					1072:1074	FRR	1072:1074	FRR	1072:1074	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).					
37028725	9	40	theme	high	1046:1049	arg1	ratio					1065:1069	high flux recovery ratio	1046:1069	a high flux recovery ratio (FRR) value (82%)	1044:1087	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).					
37028725	1	41	dep	water	193:197	arg1	treatment					214:222	treatment	214:222	treatment	214:222	Nanofiltration (NF) membranes are promising media for water and wastewater treatment; however, they suffer from their hydrophobic nature and low permeability.					
37028725	9	42	theme	recovery	1056:1063	arg1	FRR					1072:1074	FRR	1072:1074	FRR	1072:1074	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).					
37028725	9	42	theme	recovery	1056:1063	arg1	ratio					1065:1069	high flux recovery ratio	1046:1069	a high flux recovery ratio (FRR) value (82%)	1044:1087	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).					
37028725	3	43	theme	@	438:438	arg1	nanocomposite					442:454	Fe3O4@GA nanocomposite	433:454	Fe3O4@GA nanocomposite	433:454	First, Fe3O4@GA nanocomposite was synthesized by the co-precipitation approach and then its morphology, elemental composition, thermal stability, and functional groups were characterized by various analyses.					
37028725	5	44	theme	phase	794:798	arg1	method					818:823	a nonsolvent-induced phase separation (NIPS) method	773:823	a nonsolvent-induced phase separation (NIPS) method	773:823	The bare and modified membranes were fabricated by a nonsolvent-induced phase separation (NIPS) method.					
37028725	6	45	theme	fabricated	849:858	arg1	membranes					860:868	fabricated membranes	849:868	fabricated membranes	849:868	The characteristics of fabricated membranes were assessed by mechanical strength, water contact angle, pore size, and porosity measurements.					
37028725	11	46	theme	efficient	1468:1476	arg1	procedure					1478:1486	a suitable and efficient procedure	1453:1486	a suitable and efficient procedure for modifying NF membranes	1453:1513	According to the results, adding Fe3O4@GA green nanocomposite to the membrane casting solution is a suitable and efficient procedure for modifying NF membranes.					
37028725	10	47	theme	rejection	1224:1232	arg1	rates					1234:1238	high rejection rates	1219:1238	high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic	1219:1315	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	11	48	theme	GA	1394:1395	arg1	nanocomposite					1403:1415	Fe3O4@GA green nanocomposite	1388:1415	Fe3O4@GA green nanocomposite	1388:1415	According to the results, adding Fe3O4@GA green nanocomposite to the membrane casting solution is a suitable and efficient procedure for modifying NF membranes.					
37028725	10	49	theme	Red-195	1256:1262	arg1	rates					1234:1238	high rejection rates	1219:1238	high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic	1219:1315	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	9	50	with	flux	1034:1037	arg1	value					1077:1081	a high flux recovery ratio (FRR) value	1044:1081	a high flux recovery ratio (FRR) value (82%)	1044:1087	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).					
37028725	9	50	with	flux	1034:1037	arg1	%					1086:1086	82%	1084:1086	82%	1084:1086	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).					
37028725	11	51	theme	NF	1502:1503	arg1	membranes					1505:1513	NF membranes	1502:1513	NF membranes	1502:1513	According to the results, adding Fe3O4@GA green nanocomposite to the membrane casting solution is a suitable and efficient procedure for modifying NF membranes.					
37028725	10	52	theme	%	1245:1245	arg1	Red-195					1256:1262	98% Reactive Red-195	1243:1262	98% Reactive Red-195	1243:1262	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	5	53	theme	nonsolvent-induced	775:792	arg1	method					818:823	a nonsolvent-induced phase separation (NIPS) method	773:823	a nonsolvent-induced phase separation (NIPS) method	773:823	The bare and modified membranes were fabricated by a nonsolvent-induced phase separation (NIPS) method.					
37028725	10	54	theme	Reactive	1269:1276	arg1	Blue-19					1278:1284	95% Reactive Blue-19	1265:1284	95% Reactive Blue-19	1265:1284	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	5	55	theme	separation	800:809	arg1	method					818:823	a nonsolvent-induced phase separation (NIPS) method	773:823	a nonsolvent-induced phase separation (NIPS) method	773:823	The bare and modified membranes were fabricated by a nonsolvent-induced phase separation (NIPS) method.					
37028725	6	56	theme	water	908:912	arg1	angle					922:926	water contact angle	908:926	water contact angle	908:926	The characteristics of fabricated membranes were assessed by mechanical strength, water contact angle, pore size, and porosity measurements.					
37028725	0	57	theme	pollutants	127:136	arg1	performance					78:88	antifouling performance	66:88	antifouling performance	66:88	Fe3O4@Gum Arabic modified polyvinyl chloride membranes to improve antifouling performance and separation efficiency of organic pollutants.					
37028725	0	57	theme	pollutants	127:136	arg1	efficiency					105:114	separation efficiency	94:114	separation efficiency	94:114	Fe3O4@Gum Arabic modified polyvinyl chloride membranes to improve antifouling performance and separation efficiency of organic pollutants.					
37028725	10	58	theme	95	1265:1266	arg1	%					1267:1267	%	1267:1267	%	1267:1267	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	7	59	theme	Fe3O4	979:983	arg1	membrane					992:999	The optimum Fe3O4@GA/PVC membrane	967:999	The optimum Fe3O4@GA/PVC membrane	967:999	The optimum Fe3O4@GA/PVC membrane had a 52 L m-2.					
37028725	7	60	theme	GA/PVC	985:990	arg1	membrane					992:999	The optimum Fe3O4@GA/PVC membrane	967:999	The optimum Fe3O4@GA/PVC membrane	967:999	The optimum Fe3O4@GA/PVC membrane had a 52 L m-2.					
37028725	10	61	theme	filtration	1100:1109	arg1	experiment					1111:1120	the filtration experiment	1096:1120	the filtration experiment	1096:1120	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	11	62	theme	Fe3O4	1388:1392	arg1	nanocomposite					1403:1415	Fe3O4@GA green nanocomposite	1388:1415	Fe3O4@GA green nanocomposite	1388:1415	According to the results, adding Fe3O4@GA green nanocomposite to the membrane casting solution is a suitable and efficient procedure for modifying NF membranes.					
37028725	6	63	theme	porosity	944:951	arg1	measurements					953:964	porosity measurements	944:964	porosity measurements	944:964	The characteristics of fabricated membranes were assessed by mechanical strength, water contact angle, pore size, and porosity measurements.					
37028725	4	64	theme	prepared	644:651	arg1	nanocomposite					653:665	the prepared nanocomposite	640:665	the prepared nanocomposite	640:665	Next, the prepared nanocomposite was added to the casting solution of the PVC membrane.					
37028725	3	65	theme	elemental	530:538	arg1	composition					540:550	elemental composition	530:550	elemental composition	530:550	First, Fe3O4@GA nanocomposite was synthesized by the co-precipitation approach and then its morphology, elemental composition, thermal stability, and functional groups were characterized by various analyses.					
37028725	5	66	theme	NIPS	812:815	arg1	method					818:823	a nonsolvent-induced phase separation (NIPS) method	773:823	a nonsolvent-induced phase separation (NIPS) method	773:823	The bare and modified membranes were fabricated by a nonsolvent-induced phase separation (NIPS) method.					
37028725	6	67	theme	pore	929:932	arg1	size					934:937	pore size	929:937	pore size	929:937	The characteristics of fabricated membranes were assessed by mechanical strength, water contact angle, pore size, and porosity measurements.					
37028725	10	68	theme	96	1291:1292	arg1	%					1293:1293	%	1293:1293	%	1293:1293	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	0	69	theme	separation	94:103	arg1	efficiency					105:114	separation efficiency	94:114	separation efficiency	94:114	Fe3O4@Gum Arabic modified polyvinyl chloride membranes to improve antifouling performance and separation efficiency of organic pollutants.					
37028725	9	70	theme	flux	1051:1054	arg1	FRR					1072:1074	FRR	1072:1074	FRR	1072:1074	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).					
37028725	9	70	theme	flux	1051:1054	arg1	ratio					1065:1069	high flux recovery ratio	1046:1069	a high flux recovery ratio (FRR) value (82%)	1044:1087	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).					
37028725	4	71	theme	casting	684:690	arg1	solution					692:699	the casting solution	680:699	the casting solution of the PVC membrane	680:719	Next, the prepared nanocomposite was added to the casting solution of the PVC membrane.					
37028725	10	72	theme	@	1146:1146	arg1	membrane					1154:1161	the Fe3O4@GA/PVC membrane	1137:1161	the Fe3O4@GA/PVC membrane	1137:1161	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	5	73	theme	bare	726:729	arg1	membranes					744:752	The bare and modified membranes	722:752	The bare and modified membranes	722:752	The bare and modified membranes were fabricated by a nonsolvent-induced phase separation (NIPS) method.					
37028725	2	74	theme	Gum	389:391	arg1	Arabic					393:398	iron (III) oxide@Gum Arabic	372:398	iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite	372:423	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	2	74	theme	Gum	389:391	arg1	GA					407:408	Fe3O4@GA	401:408	Fe3O4@GA	401:408	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	10	75	theme	Rifampicin	1295:1304	arg1	rates					1234:1238	high rejection rates	1219:1238	high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic	1219:1315	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	10	76	theme	organic	1187:1193	arg1	contaminants					1195:1206	organic contaminants	1187:1206	organic contaminants	1187:1206	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	5	77	theme	modified	735:742	arg1	membranes					744:752	The bare and modified membranes	722:752	The bare and modified membranes	722:752	The bare and modified membranes were fabricated by a nonsolvent-induced phase separation (NIPS) method.					
37028725	2	78	theme	oxide	383:387	arg1	Arabic					393:398	iron (III) oxide@Gum Arabic	372:398	iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite	372:423	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	2	78	theme	oxide	383:387	arg1	GA					407:408	Fe3O4@GA	401:408	Fe3O4@GA	401:408	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	10	79	theme	Fe3O4	1332:1336	arg1	membrane					1345:1352	Fe3O4@GA/PVC membrane	1332:1352	Fe3O4@GA/PVC membrane	1332:1352	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	9	80	theme	bar-1	1022:1026	arg1	flux					1034:1037	bar-1 water flux	1022:1037	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).	1022:1088	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).					
37028725	2	81	theme	NF	344:345	arg1	membrane					347:354	the polyvinyl chloride (PVC) NF membrane	315:354	the polyvinyl chloride (PVC) NF membrane	315:354	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	0	82	theme	polyvinyl	26:34	arg1	membranes					45:53	polyvinyl chloride membranes	26:53	polyvinyl chloride membranes	26:53	Fe3O4@Gum Arabic modified polyvinyl chloride membranes to improve antifouling performance and separation efficiency of organic pollutants.					
37028725	2	83	theme	@	406:406	arg1	Arabic					393:398	iron (III) oxide@Gum Arabic	372:398	iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite	372:423	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	2	83	theme	@	406:406	arg1	GA					407:408	Fe3O4@GA	401:408	Fe3O4@GA	401:408	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	10	84	theme	high	1219:1222	arg1	rates					1234:1238	high rejection rates	1219:1238	high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic	1219:1315	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	2	85	theme	iron	372:375	arg1	Arabic					393:398	iron (III) oxide@Gum Arabic	372:398	iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite	372:423	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	2	85	theme	iron	372:375	arg1	GA					407:408	Fe3O4@GA	401:408	Fe3O4@GA	401:408	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	11	86	theme	green	1397:1401	arg1	nanocomposite					1403:1415	Fe3O4@GA green nanocomposite	1388:1415	Fe3O4@GA green nanocomposite	1388:1415	According to the results, adding Fe3O4@GA green nanocomposite to the membrane casting solution is a suitable and efficient procedure for modifying NF membranes.					
37028725	2	87	theme	polyvinyl	319:327	arg1	PVC					339:341	PVC	339:341	PVC	339:341	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	2	87	theme	polyvinyl	319:327	arg1	chloride					329:336	polyvinyl chloride	319:336	the polyvinyl chloride (PVC) NF membrane	315:354	For this reason, the polyvinyl chloride (PVC) NF membrane was modified by iron (III) oxide@Gum Arabic (Fe3O4@GA) nanocomposite.					
37028725	11	88	theme	membrane	1424:1431	arg1	solution					1441:1448	the membrane casting solution	1420:1448	the membrane casting solution	1420:1448	According to the results, adding Fe3O4@GA green nanocomposite to the membrane casting solution is a suitable and efficient procedure for modifying NF membranes.					
37028725	10	89	theme	GA/PVC	1338:1343	arg1	membrane					1345:1352	Fe3O4@GA/PVC membrane	1332:1352	Fe3O4@GA/PVC membrane	1332:1352	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	4	90	theme	PVC	708:710	arg1	membrane					712:719	the PVC membrane	704:719	the PVC membrane	704:719	Next, the prepared nanocomposite was added to the casting solution of the PVC membrane.					
37028725	10	91	theme	98	1243:1244	arg1	%					1245:1245	%	1245:1245	%	1245:1245	Also, the filtration experiment exhibited that the Fe3O4@GA/PVC membrane could remarkably remove organic contaminants, achieving high rejection rates of 98% Reactive Red-195, 95% Reactive Blue-19, and 96% Rifampicin antibiotic by 0.25 wt% of Fe3O4@GA/PVC membrane.					
37028725	3	92	theme	GA	439:440	arg1	nanocomposite					442:454	Fe3O4@GA nanocomposite	433:454	Fe3O4@GA nanocomposite	433:454	First, Fe3O4@GA nanocomposite was synthesized by the co-precipitation approach and then its morphology, elemental composition, thermal stability, and functional groups were characterized by various analyses.					
37028725	9	93	theme	ratio	1065:1069	arg1	value					1077:1081	a high flux recovery ratio (FRR) value	1044:1081	a high flux recovery ratio (FRR) value (82%)	1044:1087	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).					
37028725	9	93	theme	ratio	1065:1069	arg1	%					1086:1086	82%	1084:1086	82%	1084:1086	bar-1 water flux with a high flux recovery ratio (FRR) value (82%).					
37028725	0	94	theme	@	5:5	arg1	Arabic					10:15	Fe3O4@Gum Arabic	0:15	Fe3O4@Gum Arabic	0:15	Fe3O4@Gum Arabic modified polyvinyl chloride membranes to improve antifouling performance and separation efficiency of organic pollutants.					
36063576	5	0	theme	5 	929:930	arg1	%					936:936	5 %-15 %	929:936	5 %-15 %	929:936	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	5	0	theme	5 	929:930	arg1	addition					919:926	CSH addition	915:926	CSH addition (5 %-15 %)	915:937	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	2	1	theme	low	495:497	arg1	biodegradability					499:514	low biodegradability	495:514	low biodegradability	495:514	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	7	2	theme	bone	1524:1527	arg1	defects					1529:1535	bone defects	1524:1535	bone defects in situations of trauma and diseased bone	1524:1577	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	6	3	theme	α-TCP-based	1102:1112	arg1	composites					1114:1123	These α-TCP-based composites	1096:1123	These α-TCP-based composites	1096:1123	These α-TCP-based composites could also accelerate the biodegradation rate and new bone regeneration in rabbit lateral femoral bone defect models in vivo.					
36063576	5	4	theme	%	931:931	arg1	%					936:936	5 %-15 %	929:936	5 %-15 %	929:936	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	5	4	theme	%	931:931	arg1	addition					919:926	CSH addition	915:926	CSH addition (5 %-15 %)	915:937	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	7	5	theme	physicochemical	1390:1404	arg1	properties					1406:1415	the physicochemical properties	1386:1415	the physicochemical properties	1386:1415	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	0	6	theme	bone	99:102	arg1	repair					104:109	bone repair	99:109	bone repair	99:109	A new injectable quick hardening anti-collapse bone cement allows for improving biodegradation and bone repair.					
36063576	3	7	theme	liquid	710:715	arg1	optimization					723:734	liquid phase optimization	710:734	liquid phase optimization	710:734	This study aimed to develop new injectable α-TCP-based bone cements via strontium doping, α-calcium sulfate hemihydrate (CSH) addition and liquid phase optimization.					
36063576	4	8	theme	higher	861:866	arg1	resistance					868:877	higher resistance	861:877	higher resistance to structure collapse	861:899	A combination of citric acid and chitosan was identified to facilitate the injectable and anti-washout properties, enabling higher resistance to structure collapse.					
36063576	5	9	theme	setting	972:978	arg1	time					980:983	the setting time	968:983	the setting time (5-20 min)	968:994	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	5	9	theme	setting	972:978	arg1	5-20 min					986:993	5-20 min	986:993	5-20 min	986:993	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	7	10	theme	α-TCP-based	1447:1457	arg1	bone					1459:1462	α-TCP-based bone cements	1447:1470	α-TCP-based bone cements	1447:1470	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	6	11	theme	new	1175:1177	arg1	regeneration					1184:1195	new bone regeneration	1175:1195	new bone regeneration	1175:1195	These α-TCP-based composites could also accelerate the biodegradation rate and new bone regeneration in rabbit lateral femoral bone defect models in vivo.					
36063576	2	12	theme	phosphate	316:324	arg1	powders					334:340	α-tricalcium phosphate (α-TCP) powders	303:340	α-tricalcium phosphate (α-TCP) powders	303:340	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	6	13	from	regeneration	1184:1195	arg1	models					1235:1240	rabbit lateral femoral bone defect models	1200:1240	rabbit lateral femoral bone defect models	1200:1240	These α-TCP-based composites could also accelerate the biodegradation rate and new bone regeneration in rabbit lateral femoral bone defect models in vivo.					
36063576	7	14	theme	ion	1288:1290	arg1	doping					1292:1297	foreign ion doping	1280:1297	foreign ion doping	1280:1297	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	2	15	theme	α-tricalcium	303:314	arg1	phosphate					316:324	α-tricalcium phosphate	303:324	α-tricalcium phosphate (α-TCP) powders	303:340	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	2	15	theme	α-tricalcium	303:314	arg1	α-TCP					327:331	α-TCP	327:331	α-TCP	327:331	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	6	16	dep	rate	1166:1169	arg1	the					1147:1149	the	1147:1149	the	1147:1149	These α-TCP-based composites could also accelerate the biodegradation rate and new bone regeneration in rabbit lateral femoral bone defect models in vivo.					
36063576	0	17	theme	bone	47:50	arg1	cement					52:57	A new injectable quick hardening anti-collapse bone cement	0:57	A new injectable quick hardening anti-collapse bone cement	0:57	A new injectable quick hardening anti-collapse bone cement allows for improving biodegradation and bone repair.					
36063576	7	18	theme	promising	1487:1495	arg1	biomaterials					1497:1508	promising biomaterials	1487:1508	promising biomaterials	1487:1508	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	2	19	theme	poor	460:463	arg1	properties					479:488	poor anti-collapse properties	460:488	poor anti-collapse properties	460:488	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	2	20	theme	CDHA	423:426	arg1	cement					429:434	hydraulic calcium-deficient hydroxyapatite (CDHA) cement	379:434	hydraulic calcium-deficient hydroxyapatite (CDHA) cement	379:434	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	0	21	theme	new	2:4	arg1	cement					52:57	A new injectable quick hardening anti-collapse bone cement	0:57	A new injectable quick hardening anti-collapse bone cement	0:57	A new injectable quick hardening anti-collapse bone cement allows for improving biodegradation and bone repair.					
36063576	7	22	theme	secondary	1300:1308	arg1	addition					1316:1323	secondary phase addition	1300:1323	secondary phase addition	1300:1323	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	4	23	theme	acid	761:764	arg1	combination					739:749	A combination	737:749	A combination of citric acid and chitosan	737:777	A combination of citric acid and chitosan was identified to facilitate the injectable and anti-washout properties, enabling higher resistance to structure collapse.					
36063576	7	24	theme	liquid	1329:1334	arg1	optimization					1343:1354	liquid medium optimization	1329:1354	liquid medium optimization	1329:1354	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	6	25	theme	femoral	1215:1221	arg1	models					1235:1240	rabbit lateral femoral bone defect models	1200:1240	rabbit lateral femoral bone defect models	1200:1240	These α-TCP-based composites could also accelerate the biodegradation rate and new bone regeneration in rabbit lateral femoral bone defect models in vivo.					
36063576	4	26	theme	structure	882:890	arg1	collapse					892:899	structure collapse	882:899	structure collapse	882:899	A combination of citric acid and chitosan was identified to facilitate the injectable and anti-washout properties, enabling higher resistance to structure collapse.					
36063576	4	27	theme	citric	754:759	arg1	acid					761:764	citric acid	754:764	citric acid	754:764	A combination of citric acid and chitosan was identified to facilitate the injectable and anti-washout properties, enabling higher resistance to structure collapse.					
36063576	6	28	from	rate	1166:1169	arg1	models					1235:1240	rabbit lateral femoral bone defect models	1200:1240	rabbit lateral femoral bone defect models	1200:1240	These α-TCP-based composites could also accelerate the biodegradation rate and new bone regeneration in rabbit lateral femoral bone defect models in vivo.					
36063576	2	29	theme	hydroxyapatite	407:420	arg1	cement					429:434	hydraulic calcium-deficient hydroxyapatite (CDHA) cement	379:434	hydraulic calcium-deficient hydroxyapatite (CDHA) cement	379:434	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	0	30	theme	quick	17:21	arg1	cement					52:57	A new injectable quick hardening anti-collapse bone cement	0:57	A new injectable quick hardening anti-collapse bone cement	0:57	A new injectable quick hardening anti-collapse bone cement allows for improving biodegradation and bone repair.					
36063576	2	31	theme	hydraulic	379:387	arg1	cement					429:434	hydraulic calcium-deficient hydroxyapatite (CDHA) cement	379:434	hydraulic calcium-deficient hydroxyapatite (CDHA) cement	379:434	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	4	32	theme	chitosan	770:777	arg1	combination					739:749	A combination	737:749	A combination of citric acid and chitosan	737:777	A combination of citric acid and chitosan was identified to facilitate the injectable and anti-washout properties, enabling higher resistance to structure collapse.					
36063576	5	33	theme	compressive	1016:1026	arg1	strength					1028:1035	the compressive strength	1012:1035	the compressive strength (10-14 MPa)	1012:1047	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	5	33	theme	compressive	1016:1026	arg1	10-14 MPa					1038:1046	10-14 MPa	1038:1046	10-14 MPa	1038:1046	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	2	34	theme	long	441:444	arg1	time					454:457	its long setting time	437:457	its long setting time	437:457	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	2	35	theme	setting	446:452	arg1	time					454:457	its long setting time	437:457	its long setting time	437:457	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	6	36	theme	rabbit	1200:1205	arg1	models					1235:1240	rabbit lateral femoral bone defect models	1200:1240	rabbit lateral femoral bone defect models	1200:1240	These α-TCP-based composites could also accelerate the biodegradation rate and new bone regeneration in rabbit lateral femoral bone defect models in vivo.					
36063576	3	37	theme	sulfate	671:677	arg1	addition					697:704	α-calcium sulfate hemihydrate (CSH) addition	661:704	α-calcium sulfate hemihydrate (CSH) addition	661:704	This study aimed to develop new injectable α-TCP-based bone cements via strontium doping, α-calcium sulfate hemihydrate (CSH) addition and liquid phase optimization.					
36063576	6	38	theme	biodegradation	1151:1164	arg1	rate					1166:1169	biodegradation rate	1151:1169	biodegradation rate	1151:1169	These α-TCP-based composites could also accelerate the biodegradation rate and new bone regeneration in rabbit lateral femoral bone defect models in vivo.					
36063576	7	39	theme	bone	1459:1462	arg1	properties					1406:1415	the physicochemical properties	1386:1415	the physicochemical properties	1386:1415	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	7	39	theme	bone	1459:1462	arg1	performance					1432:1442	biological performance	1421:1442	biological performance	1421:1442	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	2	40	theme	calcium-deficient	389:405	arg1	cement					429:434	hydraulic calcium-deficient hydroxyapatite (CDHA) cement	379:434	hydraulic calcium-deficient hydroxyapatite (CDHA) cement	379:434	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	0	41	theme	anti-collapse	33:45	arg1	cement					52:57	A new injectable quick hardening anti-collapse bone cement	0:57	A new injectable quick hardening anti-collapse bone cement	0:57	A new injectable quick hardening anti-collapse bone cement allows for improving biodegradation and bone repair.					
36063576	7	42	theme	phase	1310:1314	arg1	addition					1316:1323	secondary phase addition	1300:1323	secondary phase addition	1300:1323	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	2	43	theme	applications	557:568	arg1	variety					537:543	a variety	535:543	a variety of clinical applications	535:568	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	2	43	theme	applications	557:568	arg1	applications					557:568	clinical applications	548:568	clinical applications	548:568	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	3	44	theme	hemihydrate	679:689	arg1	addition					697:704	α-calcium sulfate hemihydrate (CSH) addition	661:704	α-calcium sulfate hemihydrate (CSH) addition	661:704	This study aimed to develop new injectable α-TCP-based bone cements via strontium doping, α-calcium sulfate hemihydrate (CSH) addition and liquid phase optimization.					
36063576	3	45	theme	α-TCP-based	614:624	arg1	bone					626:629	new injectable α-TCP-based bone	599:629	new injectable α-TCP-based bone	599:629	This study aimed to develop new injectable α-TCP-based bone cements via strontium doping, α-calcium sulfate hemihydrate (CSH) addition and liquid phase optimization.					
36063576	5	46	theme	CSH	915:917	arg1	%					936:936	5 %-15 %	929:936	5 %-15 %	929:936	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	5	46	theme	CSH	915:917	arg1	addition					919:926	CSH addition	915:926	CSH addition (5 %-15 %)	915:937	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	1	47	theme	considerable	222:233	arg1	interest					244:251	considerable clinical interest	222:251	considerable clinical interest	222:251	The development of injectable cement-like biomaterials via a minimally invasive approach has always attracted considerable clinical interest for modern bone regeneration and repair.					
36063576	0	48	theme	hardening	23:31	arg1	cement					52:57	A new injectable quick hardening anti-collapse bone cement	0:57	A new injectable quick hardening anti-collapse bone cement	0:57	A new injectable quick hardening anti-collapse bone cement allows for improving biodegradation and bone repair.					
36063576	2	49	theme	clinical	548:555	arg1	applications					557:568	clinical applications	548:568	clinical applications	548:568	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	5	50	theme	buffer	1081:1086	arg1	medium					1088:1093	an aqueous buffer medium	1070:1093	an aqueous buffer medium	1070:1093	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	1	51	theme	clinical	235:242	arg1	interest					244:251	considerable clinical interest	222:251	considerable clinical interest	222:251	The development of injectable cement-like biomaterials via a minimally invasive approach has always attracted considerable clinical interest for modern bone regeneration and repair.					
36063576	7	52	theme	trauma	1554:1559	arg1	situations					1540:1549	situations	1540:1549	situations of trauma and diseased bone	1540:1577	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	3	53	theme	α-calcium	661:669	arg1	addition					697:704	α-calcium sulfate hemihydrate (CSH) addition	661:704	α-calcium sulfate hemihydrate (CSH) addition	661:704	This study aimed to develop new injectable α-TCP-based bone cements via strontium doping, α-calcium sulfate hemihydrate (CSH) addition and liquid phase optimization.					
36063576	3	54	theme	new	599:601	arg1	bone					626:629	new injectable α-TCP-based bone	599:629	new injectable α-TCP-based bone	599:629	This study aimed to develop new injectable α-TCP-based bone cements via strontium doping, α-calcium sulfate hemihydrate (CSH) addition and liquid phase optimization.					
36063576	7	55	dep	bone	1459:1462	arg1	cements					1464:1470	cements	1464:1470	cements	1464:1470	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	2	56	theme	anti-collapse	465:477	arg1	properties					479:488	poor anti-collapse properties	460:488	poor anti-collapse properties	460:488	Although α-tricalcium phosphate (α-TCP) powders may readily react with water to form hydraulic calcium-deficient hydroxyapatite (CDHA) cement, its long setting time, poor anti-collapse properties, and low biodegradability are suboptimal for a variety of clinical applications.					
36063576	7	57	theme	medium	1336:1341	arg1	optimization					1343:1354	liquid medium optimization	1329:1354	liquid medium optimization	1329:1354	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	7	58	theme	biological	1421:1430	arg1	performance					1432:1442	biological performance	1421:1442	biological performance	1421:1442	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	3	59	theme	injectable	603:612	arg1	bone					626:629	new injectable α-TCP-based bone	599:629	new injectable α-TCP-based bone	599:629	This study aimed to develop new injectable α-TCP-based bone cements via strontium doping, α-calcium sulfate hemihydrate (CSH) addition and liquid phase optimization.					
36063576	7	60	theme	bone	1574:1577	arg1	situations					1540:1549	situations	1540:1549	situations of trauma and diseased bone	1540:1577	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	6	61	theme	lateral	1207:1213	arg1	models					1235:1240	rabbit lateral femoral bone defect models	1200:1240	rabbit lateral femoral bone defect models	1200:1240	These α-TCP-based composites could also accelerate the biodegradation rate and new bone regeneration in rabbit lateral femoral bone defect models in vivo.					
36063576	4	62	theme	injectable	812:821	arg1	properties					840:849	the injectable and anti-washout properties	808:849	the injectable and anti-washout properties	808:849	A combination of citric acid and chitosan was identified to facilitate the injectable and anti-washout properties, enabling higher resistance to structure collapse.					
36063576	3	63	theme	strontium	643:651	arg1	doping					653:658	strontium doping	643:658	strontium doping	643:658	This study aimed to develop new injectable α-TCP-based bone cements via strontium doping, α-calcium sulfate hemihydrate (CSH) addition and liquid phase optimization.					
36063576	7	64	theme	foreign	1280:1286	arg1	doping					1292:1297	foreign ion doping	1280:1297	foreign ion doping	1280:1297	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	7	65	from	defects	1529:1535	arg1	situations					1540:1549	situations	1540:1549	situations of trauma and diseased bone	1540:1577	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	6	66	theme	bone	1179:1182	arg1	regeneration					1184:1195	new bone regeneration	1175:1195	new bone regeneration	1175:1195	These α-TCP-based composites could also accelerate the biodegradation rate and new bone regeneration in rabbit lateral femoral bone defect models in vivo.					
36063576	6	67	theme	defect	1228:1233	arg1	models					1235:1240	rabbit lateral femoral bone defect models	1200:1240	rabbit lateral femoral bone defect models	1200:1240	These α-TCP-based composites could also accelerate the biodegradation rate and new bone regeneration in rabbit lateral femoral bone defect models in vivo.					
36063576	6	68	theme	bone	1223:1226	arg1	models					1235:1240	rabbit lateral femoral bone defect models	1200:1240	rabbit lateral femoral bone defect models	1200:1240	These α-TCP-based composites could also accelerate the biodegradation rate and new bone regeneration in rabbit lateral femoral bone defect models in vivo.					
36063576	5	69	theme	aqueous	1073:1079	arg1	medium					1088:1093	an aqueous buffer medium	1070:1093	an aqueous buffer medium	1070:1093	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	1	70	theme	injectable	131:140	arg1	biomaterials					154:165	injectable cement-like biomaterials	131:165	injectable cement-like biomaterials	131:165	The development of injectable cement-like biomaterials via a minimally invasive approach has always attracted considerable clinical interest for modern bone regeneration and repair.					
36063576	5	71	from	incubation	1056:1065	arg1	medium					1088:1093	an aqueous buffer medium	1070:1093	an aqueous buffer medium	1070:1093	Furthermore, CSH addition (5 %-15 %) was favorable for shortening the setting time (5-20 min) and maintaining the compressive strength (10-14 MPa) during incubation in an aqueous buffer medium.					
36063576	3	72	theme	CSH	692:694	arg1	addition					697:704	α-calcium sulfate hemihydrate (CSH) addition	661:704	α-calcium sulfate hemihydrate (CSH) addition	661:704	This study aimed to develop new injectable α-TCP-based bone cements via strontium doping, α-calcium sulfate hemihydrate (CSH) addition and liquid phase optimization.					
36063576	4	73	theme	anti-washout	827:838	arg1	properties					840:849	the injectable and anti-washout properties	808:849	the injectable and anti-washout properties	808:849	A combination of citric acid and chitosan was identified to facilitate the injectable and anti-washout properties, enabling higher resistance to structure collapse.					
36063576	1	74	theme	cement-like	142:152	arg1	biomaterials					154:165	injectable cement-like biomaterials	131:165	injectable cement-like biomaterials	131:165	The development of injectable cement-like biomaterials via a minimally invasive approach has always attracted considerable clinical interest for modern bone regeneration and repair.					
36063576	1	75	theme	invasive	183:190	arg1	approach					192:199	a minimally invasive approach	171:199	a minimally invasive approach	171:199	The development of injectable cement-like biomaterials via a minimally invasive approach has always attracted considerable clinical interest for modern bone regeneration and repair.					
36063576	1	76	theme	modern	257:262	arg1	regeneration					269:280	modern bone regeneration	257:280	modern bone regeneration	257:280	The development of injectable cement-like biomaterials via a minimally invasive approach has always attracted considerable clinical interest for modern bone regeneration and repair.					
36063576	3	77	theme	phase	717:721	arg1	optimization					723:734	liquid phase optimization	710:734	liquid phase optimization	710:734	This study aimed to develop new injectable α-TCP-based bone cements via strontium doping, α-calcium sulfate hemihydrate (CSH) addition and liquid phase optimization.					
36063576	0	78	theme	injectable	6:15	arg1	cement					52:57	A new injectable quick hardening anti-collapse bone cement	0:57	A new injectable quick hardening anti-collapse bone cement	0:57	A new injectable quick hardening anti-collapse bone cement allows for improving biodegradation and bone repair.					
36063576	7	79	theme	diseased	1565:1572	arg1	bone					1574:1577	diseased bone	1565:1577	diseased bone	1565:1577	Our studies demonstrate that foreign ion doping, secondary phase addition and liquid medium optimization could synergistically improve the physicochemical properties and biological performance of α-TCP-based bone cements, which will be promising biomaterials for repairing bone defects in situations of trauma and diseased bone.					
36063576	1	80	theme	biomaterials	154:165	arg1	development					116:126	The development	112:126	The development of injectable cement-like biomaterials via a minimally invasive approach	112:199	The development of injectable cement-like biomaterials via a minimally invasive approach has always attracted considerable clinical interest for modern bone regeneration and repair.					
36063576	1	81	theme	bone	264:267	arg1	regeneration					269:280	modern bone regeneration	257:280	modern bone regeneration	257:280	The development of injectable cement-like biomaterials via a minimally invasive approach has always attracted considerable clinical interest for modern bone regeneration and repair.					
37290507	3	0	theme	particles	551:559	arg1	nature					518:523	The nature	514:523	The nature of synthesized iron oxide particles	514:559	The nature of synthesized iron oxide particles was confirmed as magnetite using XRD diffraction.					
37290507	3	0	theme	particles	551:559	arg1	magnetite					578:586	magnetite	578:586	magnetite using XRD diffraction	578:608	The nature of synthesized iron oxide particles was confirmed as magnetite using XRD diffraction.					
37290507	11	1	theme	sewage	1502:1507	arg1	water					1509:1513	sewage water	1502:1513	sewage water spiked with SMX	1502:1529	Fabricated beads were successfully applied in a floating bed column in which the beads were allowed to float in sewage water spiked with SMX for 40 min.					
37290507	3	2	theme	iron	540:543	arg1	particles					551:559	synthesized iron oxide particles	528:559	synthesized iron oxide particles	528:559	The nature of synthesized iron oxide particles was confirmed as magnetite using XRD diffraction.					
37290507	5	3	theme	initial	855:861	arg1	30 mg L-1					882:890	30 mg L-1	882:890	30 mg L-1	882:890	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	5	3	theme	initial	855:861	arg1	concentration					867:879	initial SMX concentration	855:879	initial SMX concentration (30 mg L-1)	855:891	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	8	4	dep	cleaving	1078:1085	arg1	neither					1063:1069	neither	1063:1069	neither	1063:1069	SMX degradation was initiated neither by the cleaving of C-S nor C-N followed by some chemical reactions.					
37290507	14	5	theme	active	1800:1805	arg1	sites					1807:1811	active sites	1800:1811	active sites	1800:1811	It was found that the degradation efficiency was attributed to a stable structure, textural property, active sites and *OH radicals.					
37290507	0	6	theme	bed	129:131	arg1	reactor					133:139	bed reactor	129:139	bed reactor	129:139	Two-step fabrication of cellulose embedded Fe3O4/Fe3+ composite beads as catalyst in degradation of sulfamethoxazole in floating bed reactor.					
37290507	1	7	theme	sodium	207:212	arg1	cellulose					228:236	sodium carboxymethyl cellulose	207:236	sodium carboxymethyl cellulose	207:236	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	6	8	theme	%	948:948	arg1	SMX					950:952	81.89% SMX	943:952	81.89% SMX	943:952	The results showed that under optimal conditions 81.89% SMX degraded in 40 min using H2O2.					
37290507	4	9	theme	oxide	655:659	arg1	particles					661:669	Fe3+ and iron oxide particles	641:669	Fe3+ and iron oxide particles with CMC polymer	641:686	The structural arrangement of Fe3+ and iron oxide particles with CMC polymer was discussed.					
37290507	5	10	theme	SMX	731:733	arg1	efficiency					747:756	SMX degradation efficiency	731:756	SMX degradation efficiency	731:756	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	0	11	theme	cellulose	24:32	arg1	fabrication					9:19	Two-step fabrication	0:19	Two-step fabrication of cellulose	0:32	Two-step fabrication of cellulose embedded Fe3O4/Fe3+ composite beads as catalyst in degradation of sulfamethoxazole in floating bed reactor.					
37290507	3	12	theme	XRD	594:596	arg1	diffraction					598:608	XRD diffraction	594:608	XRD diffraction	594:608	The nature of synthesized iron oxide particles was confirmed as magnetite using XRD diffraction.					
37290507	14	13	theme	stable	1763:1768	arg1	structure					1770:1778	a stable structure	1761:1778	a stable structure	1761:1778	It was found that the degradation efficiency was attributed to a stable structure, textural property, active sites and *OH radicals.					
37290507	11	14	theme	bed	1447:1449	arg1	column					1451:1456	a floating bed column	1436:1456	a floating bed column in which the beads were allowed to float in sewage water spiked with SMX for 40 min	1436:1540	Fabricated beads were successfully applied in a floating bed column in which the beads were allowed to float in sewage water spiked with SMX for 40 min.					
37290507	9	15	theme	insufficient	1212:1223	arg1	particles					1238:1246	Fe particles	1235:1246	Fe particles	1235:1246	Complete mineralization of SMX was not achieved which could be due to an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals.					
37290507	9	15	theme	insufficient	1212:1223	arg1	amount					1225:1230	an insufficient amount	1209:1230	an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals	1209:1316	Complete mineralization of SMX was not achieved which could be due to an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals.					
37290507	1	16	theme	Fenton-like	319:329	arg1	it					302:303	it	302:303	it	302:303	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	1	16	theme	Fenton-like	319:329	arg1	catalyst					331:338	a Fenton-like catalyst	317:338	a Fenton-like catalyst	317:338	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	9	17	theme	particles	1238:1246	arg1	particles					1238:1246	Fe particles	1235:1246	Fe particles	1235:1246	Complete mineralization of SMX was not achieved which could be due to an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals.					
37290507	9	17	theme	particles	1238:1246	arg1	amount					1225:1230	an insufficient amount	1209:1230	an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals	1209:1316	Complete mineralization of SMX was not achieved which could be due to an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals.					
37290507	1	18	theme	magnetite	157:165	arg1	particles					167:175	magnetite particles	157:175	magnetite particles	157:175	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	9	19	theme	CMC	1251:1253	arg1	matrix					1255:1260	CMC matrix	1251:1260	CMC matrix that are responsible for the generation of *OH radicals	1251:1316	Complete mineralization of SMX was not achieved which could be due to an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals.					
37290507	12	20	theme	COD	1571:1573	arg1	COD					1571:1573	COD	1571:1573	COD	1571:1573	A total reduction of 79% of COD was achieved in treating sewage water.					
37290507	12	20	theme	COD	1571:1573	arg1	%					1566:1566	79%	1564:1566	79% of COD	1564:1573	A total reduction of 79% of COD was achieved in treating sewage water.					
37290507	11	21	theme	Fabricated	1390:1399	arg1	beads					1401:1405	Fabricated beads	1390:1405	Fabricated beads	1390:1405	Fabricated beads were successfully applied in a floating bed column in which the beads were allowed to float in sewage water spiked with SMX for 40 min.					
37290507	1	22	theme	aqueous	371:377	arg1	solution					379:386	aqueous solution	371:386	aqueous solution	371:386	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	2	23	theme	magnetic	458:465	arg1	beads					467:471	the Na-CMC magnetic beads	447:471	the Na-CMC magnetic beads	447:471	The surface morphology and functional groups influence of the Na-CMC magnetic beads was studied using FTIR and SEM analysis.					
37290507	0	24	theme	Two-step	0:7	arg1	fabrication					9:19	Two-step fabrication	0:19	Two-step fabrication of cellulose	0:32	Two-step fabrication of cellulose embedded Fe3O4/Fe3+ composite beads as catalyst in degradation of sulfamethoxazole in floating bed reactor.					
37290507	5	25	theme	catalyst	823:830	arg1	0.2 g L-1					840:848	0.2 g L-1	840:848	0.2 g L-1	840:848	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	5	25	theme	catalyst	823:830	arg1	dosage					832:837	catalyst dosage	823:837	catalyst dosage (0.2 g L-1)	823:849	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	12	26	theme	%	1566:1566	arg1	reduction					1551:1559	A total reduction	1543:1559	A total reduction of 79% of COD	1543:1573	A total reduction of 79% of COD was achieved in treating sewage water.					
37290507	2	27	theme	functional	416:425	arg1	groups					427:432	functional groups	416:432	functional groups	416:432	The surface morphology and functional groups influence of the Na-CMC magnetic beads was studied using FTIR and SEM analysis.					
37290507	2	27	theme	functional	416:425	arg1	beads					467:471	the Na-CMC magnetic beads	447:471	the Na-CMC magnetic beads	447:471	The surface morphology and functional groups influence of the Na-CMC magnetic beads was studied using FTIR and SEM analysis.					
37290507	5	28	theme	reaction	800:807	arg1	medium					809:814	the reaction medium	796:814	the reaction medium	796:814	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	4	29	with	particles	661:669	arg1	polymer					680:686	CMC polymer	676:686	CMC polymer	676:686	The structural arrangement of Fe3+ and iron oxide particles with CMC polymer was discussed.					
37290507	0	30	theme	composite	54:62	arg1	beads					64:68	Fe3O4/Fe3+ composite beads	43:68	Fe3O4/Fe3+ composite beads	43:68	Two-step fabrication of cellulose embedded Fe3O4/Fe3+ composite beads as catalyst in degradation of sulfamethoxazole in floating bed reactor.					
37290507	9	31	theme	OH	1306:1307	arg1	radicals					1309:1316	*OH radicals	1305:1316	*OH radicals	1305:1316	Complete mineralization of SMX was not achieved which could be due to an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals.					
37290507	9	32	theme	SMX	1166:1168	arg1	mineralization					1148:1161	Complete mineralization	1139:1161	Complete mineralization of SMX	1139:1168	Complete mineralization of SMX was not achieved which could be due to an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals.					
37290507	2	33	dep	morphology	401:410	arg1	The					389:391	The	389:391	The	389:391	The surface morphology and functional groups influence of the Na-CMC magnetic beads was studied using FTIR and SEM analysis.					
37290507	2	33	dep	morphology	401:410	arg1	influence					434:442	influence	434:442	influence	434:442	The surface morphology and functional groups influence of the Na-CMC magnetic beads was studied using FTIR and SEM analysis.					
37290507	8	34	theme	C-S	1090:1092	arg1	cleaving					1078:1085	the cleaving	1074:1085	the cleaving of C-S nor C-N followed by some chemical reactions	1074:1136	SMX degradation was initiated neither by the cleaving of C-S nor C-N followed by some chemical reactions.					
37290507	14	35	theme	OH	1818:1819	arg1	radicals					1821:1828	*OH radicals	1817:1828	*OH radicals	1817:1828	It was found that the degradation efficiency was attributed to a stable structure, textural property, active sites and *OH radicals.					
37290507	8	36	theme	C-N	1098:1100	arg1	cleaving					1078:1085	the cleaving	1074:1085	the cleaving of C-S nor C-N followed by some chemical reactions	1074:1136	SMX degradation was initiated neither by the cleaving of C-S nor C-N followed by some chemical reactions.					
37290507	1	37	used	used	309:312	arg2	it					302:303	it	302:303	it	302:303	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	1	37	used	used	309:312	arg2	catalyst					331:338	a Fenton-like catalyst	317:338	a Fenton-like catalyst	317:338	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	13	38	theme	catalytic	1678:1686	arg1	activity					1688:1695	catalytic activity	1678:1695	catalytic activity	1678:1695	The beads could be used 2-3 times with significant reduction in catalytic activity.					
37290507	9	39	theme	Complete	1139:1146	arg1	mineralization					1148:1161	Complete mineralization	1139:1161	Complete mineralization of SMX	1139:1168	Complete mineralization of SMX was not achieved which could be due to an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals.					
37290507	3	40	theme	oxide	545:549	arg1	particles					551:559	synthesized iron oxide particles	528:559	synthesized iron oxide particles	528:559	The nature of synthesized iron oxide particles was confirmed as magnetite using XRD diffraction.					
37290507	3	41	theme	synthesized	528:538	arg1	particles					551:559	synthesized iron oxide particles	528:559	synthesized iron oxide particles	528:559	The nature of synthesized iron oxide particles was confirmed as magnetite using XRD diffraction.					
37290507	14	42	theme	*	1817:1817	arg1	radicals					1821:1828	*OH radicals	1817:1828	*OH radicals	1817:1828	It was found that the degradation efficiency was attributed to a stable structure, textural property, active sites and *OH radicals.					
37290507	8	43	theme	chemical	1119:1126	arg1	reactions					1128:1136	some chemical reactions	1114:1136	some chemical reactions	1114:1136	SMX degradation was initiated neither by the cleaving of C-S nor C-N followed by some chemical reactions.					
37290507	13	44	theme	significant	1653:1663	arg1	reduction					1665:1673	significant reduction	1653:1673	significant reduction in catalytic activity	1653:1695	The beads could be used 2-3 times with significant reduction in catalytic activity.					
37290507	4	45	theme	particles	661:669	arg1	arrangement					626:636	The structural arrangement	611:636	The structural arrangement of Fe3+ and iron oxide particles with CMC polymer	611:686	The structural arrangement of Fe3+ and iron oxide particles with CMC polymer was discussed.					
37290507	10	46	theme	pseudo-first	1361:1372	arg1	kinetics					1380:1387	pseudo-first order kinetics	1361:1387	pseudo-first order kinetics	1361:1387	It was explored that degradation followed pseudo-first order kinetics.					
37290507	5	47	theme	SMX	863:865	arg1	30 mg L-1					882:890	30 mg L-1	882:890	30 mg L-1	882:890	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	5	47	theme	SMX	863:865	arg1	concentration					867:879	initial SMX concentration	855:879	initial SMX concentration (30 mg L-1)	855:891	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	4	48	theme	CMC	676:678	arg1	polymer					680:686	CMC polymer	676:686	CMC polymer	676:686	The structural arrangement of Fe3+ and iron oxide particles with CMC polymer was discussed.					
37290507	1	49	from	cross-linker	266:277	arg1	step-method					286:296	two step-method	282:296	two step-method	282:296	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	12	50	theme	total	1545:1549	arg1	reduction					1551:1559	A total reduction	1543:1559	A total reduction of 79% of COD	1543:1573	A total reduction of 79% of COD was achieved in treating sewage water.					
37290507	14	51	theme	degradation	1720:1730	arg1	efficiency					1732:1741	the degradation efficiency	1716:1741	the degradation efficiency	1716:1741	It was found that the degradation efficiency was attributed to a stable structure, textural property, active sites and *OH radicals.					
37290507	7	52	from	reduction	989:997	arg1	COD					1002:1004	COD	1002:1004	COD	1002:1004	The reduction in COD was estimated to be 81.2%.					
37290507	7	53	theme	81.2	1026:1029	arg1	%					1030:1030	81.2%	1026:1030	81.2%	1026:1030	The reduction in COD was estimated to be 81.2%.					
37290507	7	53	theme	81.2	1026:1029	arg1	reduction					989:997	The reduction	985:997	The reduction in COD	985:1004	The reduction in COD was estimated to be 81.2%.					
37290507	1	54	theme	carboxymethyl	214:226	arg1	cellulose					228:236	sodium carboxymethyl cellulose	207:236	sodium carboxymethyl cellulose	207:236	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	6	55	theme	81.89	943:947	arg1	%					948:948	%	948:948	%	948:948	The results showed that under optimal conditions 81.89% SMX degraded in 40 min using H2O2.					
37290507	0	56	from	catalyst	73:80	arg1	degradation					85:95	degradation	85:95	degradation of sulfamethoxazole	85:115	Two-step fabrication of cellulose embedded Fe3O4/Fe3+ composite beads as catalyst in degradation of sulfamethoxazole in floating bed reactor.					
37290507	4	57	theme	iron	650:653	arg1	particles					661:669	Fe3+ and iron oxide particles	641:669	Fe3+ and iron oxide particles with CMC polymer	641:686	The structural arrangement of Fe3+ and iron oxide particles with CMC polymer was discussed.					
37290507	13	58	theme	2-3	1638:1640	arg1	times					1642:1646	2-3 times	1638:1646	2-3 times	1638:1646	The beads could be used 2-3 times with significant reduction in catalytic activity.					
37290507	5	59	theme	degradation	735:745	arg1	efficiency					747:756	SMX degradation efficiency	731:756	SMX degradation efficiency	731:756	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	1	60	from	cellulose	228:236	arg1	embedded					195:202	embedded	195:202	embedded	195:202	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	11	61	from	float	1493:1497	arg1	water					1509:1513	sewage water	1502:1513	sewage water spiked with SMX	1502:1529	Fabricated beads were successfully applied in a floating bed column in which the beads were allowed to float in sewage water spiked with SMX for 40 min.					
37290507	6	62	theme	optimal	924:930	arg1	conditions					932:941	optimal conditions	924:941	optimal conditions	924:941	The results showed that under optimal conditions 81.89% SMX degraded in 40 min using H2O2.					
37290507	5	63	theme	influential	707:717	arg1	factors					719:725	The influential factors	703:725	The influential factors for SMX degradation efficiency	703:756	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	11	64	theme	floating	1438:1445	arg1	column					1451:1456	a floating bed column	1436:1456	a floating bed column in which the beads were allowed to float in sewage water spiked with SMX for 40 min	1436:1540	Fabricated beads were successfully applied in a floating bed column in which the beads were allowed to float in sewage water spiked with SMX for 40 min.					
37290507	1	65	from	embedded	195:202	arg1	study					150:154	this study	145:154	this study	145:154	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	1	65	from	embedded	195:202	arg1	cellulose					228:236	sodium carboxymethyl cellulose	207:236	sodium carboxymethyl cellulose	207:236	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	2	66	theme	SEM	500:502	arg1	analysis					504:511	FTIR and SEM analysis	491:511	FTIR and SEM analysis	491:511	The surface morphology and functional groups influence of the Na-CMC magnetic beads was studied using FTIR and SEM analysis.					
37290507	9	67	theme	Fe	1235:1236	arg1	particles					1238:1246	Fe particles	1235:1246	Fe particles	1235:1246	Complete mineralization of SMX was not achieved which could be due to an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals.					
37290507	13	68	from	reduction	1665:1673	arg1	activity					1688:1695	catalytic activity	1678:1695	catalytic activity	1678:1695	The beads could be used 2-3 times with significant reduction in catalytic activity.					
37290507	2	69	theme	FTIR	491:494	arg1	analysis					504:511	FTIR and SEM analysis	491:511	FTIR and SEM analysis	491:511	The surface morphology and functional groups influence of the Na-CMC magnetic beads was studied using FTIR and SEM analysis.					
37290507	13	70	used	used	1633:1636	arg2	beads					1618:1622	The beads	1614:1622	The beads	1614:1622	The beads could be used 2-3 times with significant reduction in catalytic activity.					
37290507	0	71	theme	sulfamethoxazole	100:115	arg1	degradation					85:95	degradation	85:95	degradation of sulfamethoxazole	85:115	Two-step fabrication of cellulose embedded Fe3O4/Fe3+ composite beads as catalyst in degradation of sulfamethoxazole in floating bed reactor.					
37290507	12	72	theme	sewage	1600:1605	arg1	water					1607:1611	sewage water	1600:1611	sewage water	1600:1611	A total reduction of 79% of COD was achieved in treating sewage water.					
37290507	10	73	theme	order	1374:1378	arg1	kinetics					1380:1387	pseudo-first order kinetics	1361:1387	pseudo-first order kinetics	1361:1387	It was explored that degradation followed pseudo-first order kinetics.					
37290507	4	74	theme	Fe3+	641:644	arg1	particles					661:669	Fe3+ and iron oxide particles	641:669	Fe3+ and iron oxide particles with CMC polymer	641:686	The structural arrangement of Fe3+ and iron oxide particles with CMC polymer was discussed.					
37290507	2	75	theme	surface	393:399	arg1	morphology					401:410	surface morphology	393:410	surface morphology	393:410	The surface morphology and functional groups influence of the Na-CMC magnetic beads was studied using FTIR and SEM analysis.					
37290507	8	76	theme	SMX	1033:1035	arg1	degradation					1037:1047	SMX degradation	1033:1047	SMX degradation	1033:1047	SMX degradation was initiated neither by the cleaving of C-S nor C-N followed by some chemical reactions.					
37290507	2	77	theme	Na-CMC	451:456	arg1	beads					467:471	the Na-CMC magnetic beads	447:471	the Na-CMC magnetic beads	447:471	The surface morphology and functional groups influence of the Na-CMC magnetic beads was studied using FTIR and SEM analysis.					
37290507	1	78	from	sulfamethoxazole	351:366	arg1	solution					379:386	aqueous solution	371:386	aqueous solution	371:386	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	1	79	from	study	150:154	arg1	embedded					195:202	embedded	195:202	embedded	195:202	In this study, magnetite particles were successfully embedded in sodium carboxymethyl cellulose as beads using FeCl3 as the cross-linker in two step-method and it was used as a Fenton-like catalyst to degrade sulfamethoxazole in aqueous solution.					
37290507	0	80	theme	Fe3O4/Fe3+	43:52	arg1	beads					64:68	Fe3O4/Fe3+ composite beads	43:68	Fe3O4/Fe3+ composite beads	43:68	Two-step fabrication of cellulose embedded Fe3O4/Fe3+ composite beads as catalyst in degradation of sulfamethoxazole in floating bed reactor.					
37290507	14	81	theme	textural	1781:1788	arg1	property					1790:1797	textural property	1781:1797	textural property	1781:1797	It was found that the degradation efficiency was attributed to a stable structure, textural property, active sites and *OH radicals.					
37290507	5	82	theme	medium	809:814	arg1	dosage					832:837	catalyst dosage	823:837	catalyst dosage (0.2 g L-1)	823:849	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	5	82	theme	medium	809:814	arg1	0.2 g L-1					840:848	0.2 g L-1	840:848	0.2 g L-1	840:848	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	5	82	theme	medium	809:814	arg1	pH					790:791	the pH	786:791	the pH of the reaction medium (4.0)	786:820	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	5	82	theme	medium	809:814	arg1	30 mg L-1					882:890	30 mg L-1	882:890	30 mg L-1	882:890	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	5	82	theme	medium	809:814	arg1	4.0					817:819	4.0	817:819	4.0	817:819	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	5	82	theme	medium	809:814	arg1	concentration					867:879	initial SMX concentration	855:879	initial SMX concentration (30 mg L-1)	855:891	The influential factors for SMX degradation efficiency were investigated including the pH of the reaction medium (4.0), catalyst dosage (0.2 g L-1) and initial SMX concentration (30 mg L-1).					
37290507	9	83	theme	*	1305:1305	arg1	radicals					1309:1316	*OH radicals	1305:1316	*OH radicals	1305:1316	Complete mineralization of SMX was not achieved which could be due to an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals.					
37290507	9	84	from	amount	1225:1230	arg1	matrix					1255:1260	CMC matrix	1251:1260	CMC matrix that are responsible for the generation of *OH radicals	1251:1316	Complete mineralization of SMX was not achieved which could be due to an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals.					
37290507	2	85	theme	beads	467:471	arg1	morphology					401:410	surface morphology	393:410	surface morphology	393:410	The surface morphology and functional groups influence of the Na-CMC magnetic beads was studied using FTIR and SEM analysis.					
37290507	2	85	theme	beads	467:471	arg1	groups					427:432	functional groups	416:432	functional groups	416:432	The surface morphology and functional groups influence of the Na-CMC magnetic beads was studied using FTIR and SEM analysis.					
37290507	2	85	theme	beads	467:471	arg1	beads					467:471	the Na-CMC magnetic beads	447:471	the Na-CMC magnetic beads	447:471	The surface morphology and functional groups influence of the Na-CMC magnetic beads was studied using FTIR and SEM analysis.					
37290507	9	86	theme	radicals	1309:1316	arg1	generation					1291:1300	the generation	1287:1300	the generation of *OH radicals	1287:1316	Complete mineralization of SMX was not achieved which could be due to an insufficient amount of Fe particles in CMC matrix that are responsible for the generation of *OH radicals.					
37290507	4	87	theme	structural	615:624	arg1	arrangement					626:636	The structural arrangement	611:636	The structural arrangement of Fe3+ and iron oxide particles with CMC polymer	611:686	The structural arrangement of Fe3+ and iron oxide particles with CMC polymer was discussed.					
36347864	6	0	dep	R2	1093:1094	arg1	0.998					1097:1101	0.998	1097:1101	0.998	1097:1101	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	6	0	dep	R2	1093:1094	arg1	0.011					1108:1112	0.011	1108:1112	0.011	1108:1112	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	1	1	theme	azithromycin	273:284	arg1	removal					262:268	the removal	258:268	the removal of azithromycin (AZT) from an aqueous solution	258:315	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	4	2	theme	determination > 0.997	706:726	arg1	coefficient					691:701	the coefficient	687:701	the coefficient of determination > 0.997	687:726	The low value of p-value (1.3101e-06) and RSD (1.873) parameters, along with the coefficient of determination > 0.997 implied that the developed model was well fitted with experimental data.					
36347864	0	3	dep	modeling	102:109	arg1	optimization					123:134	optimization	123:134	optimization	123:134	Magnetic NH2-MIL-101(Al)/Chitosan nanocomposite as a novel adsorbent for the removal of azithromycin: modeling and process optimization.					
36347864	1	4	theme	MIL/Cs	212:217	arg1	nanocomposite					197:209	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite	159:209	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs)	159:228	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	1	4	theme	MIL/Cs	212:217	arg1	NCs					225:227	MIL/Cs@Fe3O4 NCs	212:227	MIL/Cs@Fe3O4 NCs	212:227	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	3	5	theme	composite	582:590	arg1	RSM-CCD					600:606	RSM-CCD	600:606	RSM-CCD	600:606	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	3	5	theme	composite	582:590	arg1	design					592:597	central composite design	574:597	central composite design (RSM-CCD)	574:607	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	5	6	theme	adsorption	961:970	arg1	capacity					972:979	adsorption capacity	961:979	adsorption capacity	961:979	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	8	7	from	effect	1347:1352	arg1	adsorption					1374:1383	AZT adsorption	1370:1383	AZT adsorption	1370:1383	The effect of Magnesium on AZT adsorption was more complicated than other background ions.					
36347864	9	8	theme	adsorbent	1447:1455	arg1	Reuse					1434:1438	Reuse	1434:1438	Reuse of the adsorbent in 10 consecutive experiments	1434:1485	Reuse of the adsorbent in 10 consecutive experiments showed that removal efficiency was reduced by about 30.24%.					
36347864	1	9	theme	@	218:218	arg1	nanocomposite					197:209	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite	159:209	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs)	159:228	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	1	9	theme	@	218:218	arg1	NCs					225:227	MIL/Cs@Fe3O4 NCs	212:227	MIL/Cs@Fe3O4 NCs	212:227	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	10	10	theme	NCs	1579:1581	arg1	performance					1551:1561	The performance	1547:1561	The performance of MIL/Cs@Fe3O4 NCs under real conditions	1547:1603	The performance of MIL/Cs@Fe3O4 NCs under real conditions was also tested and promising results were achieved, except in the treatment of AZT from raw wastewater.					
36347864	1	11	used	used	250:253	arg2	NCs					225:227	MIL/Cs@Fe3O4 NCs	212:227	MIL/Cs@Fe3O4 NCs	212:227	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	1	11	used	used	250:253	arg2	nanocomposite					197:209	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite	159:209	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs)	159:228	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	3	12	theme	various	454:460	arg1	factors					466:472	various key factors	454:472	various key factors in the AZT adsorption process	454:502	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	4	13	theme	p-value	627:633	arg1	parameters					664:673	p-value (1.3101e-06) and RSD (1.873) parameters	627:673	p-value (1.3101e-06) and RSD (1.873) parameters	627:673	The low value of p-value (1.3101e-06) and RSD (1.873) parameters, along with the coefficient of determination > 0.997 implied that the developed model was well fitted with experimental data.					
36347864	1	14	theme	Fe3O4	219:223	arg1	nanocomposite					197:209	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite	159:209	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs)	159:228	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	1	14	theme	Fe3O4	219:223	arg1	NCs					225:227	MIL/Cs@Fe3O4 NCs	212:227	MIL/Cs@Fe3O4 NCs	212:227	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	10	15	from	wastewater	1698:1707	arg1	AZT					1685:1687	AZT	1685:1687	AZT from raw wastewater	1685:1707	The performance of MIL/Cs@Fe3O4 NCs under real conditions was also tested and promising results were achieved, except in the treatment of AZT from raw wastewater.					
36347864	10	15	from	wastewater	1698:1707	arg1	treatment					1672:1680	the treatment	1668:1680	the treatment of AZT from raw wastewater	1668:1707	The performance of MIL/Cs@Fe3O4 NCs under real conditions was also tested and promising results were achieved, except in the treatment of AZT from raw wastewater.					
36347864	1	16	from	solution	308:315	arg1	removal					262:268	the removal	258:268	the removal of azithromycin (AZT) from an aqueous solution	258:315	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	10	17	theme	AZT	1685:1687	arg1	treatment					1672:1680	the treatment	1668:1680	the treatment of AZT from raw wastewater	1668:1707	The performance of MIL/Cs@Fe3O4 NCs under real conditions was also tested and promising results were achieved, except in the treatment of AZT from raw wastewater.					
36347864	7	18	from	nature	1335:1340	arg1	spontaneous					1304:1314	spontaneous	1304:1314	spontaneous	1304:1314	ΔH° > 0, ΔS° > 0, and ∆G° < 0 indicated that AZT removal was spontaneous and endothermic in nature.					
36347864	1	19	theme	present	144:150	arg1	study					152:156	the present study	140:156	the present study	140:156	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	5	20	theme	removal	934:940	arg1	AZT					957:959	AZT	957:959	AZT	957:959	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	5	20	theme	removal	934:940	arg1	efficiency					942:951	removal efficiency	934:951	removal efficiency	934:951	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	5	20	theme	removal	934:940	arg1	238.553 mg/g					1017:1028	238.553 mg/g	1017:1028	238.553 mg/g	1017:1028	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	5	20	theme	removal	934:940	arg1	%					1011:1011	98.362 ± 3.24%	998:1011	98.362 ± 3.24%	998:1011	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	2	21	theme	Fe3O4	363:367	arg1	NCs					369:371	The as-synthesized MIL/Cs@Fe3O4 NCs	337:371	The as-synthesized MIL/Cs@Fe3O4 NCs	337:371	The as-synthesized MIL/Cs@Fe3O4 NCs was characterized by SEM, TEM, XRD, FTIR, BET, and VSM techniques.					
36347864	0	22	theme	azithromycin	88:99	arg1	removal					77:83	the removal	73:83	the removal of azithromycin	73:99	Magnetic NH2-MIL-101(Al)/Chitosan nanocomposite as a novel adsorbent for the removal of azithromycin: modeling and process optimization.					
36347864	1	23	theme	aqueous	300:306	arg1	solution					308:315	an aqueous solution	297:315	an aqueous solution	297:315	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	4	24	theme	coefficient	691:701	arg1	value					618:622	The low value	610:622	The low value of p-value (1.3101e-06) and RSD (1.873) parameters, along with the coefficient of determination > 0.997	610:726	The low value of p-value (1.3101e-06) and RSD (1.873) parameters, along with the coefficient of determination > 0.997 implied that the developed model was well fitted with experimental data.					
36347864	4	25	dep	along	676:680	arg1	with					682:685	with	682:685	with	682:685	The low value of p-value (1.3101e-06) and RSD (1.873) parameters, along with the coefficient of determination > 0.997 implied that the developed model was well fitted with experimental data.					
36347864	7	26	from	spontaneous	1304:1314	arg1	nature					1335:1340	nature	1335:1340	nature	1335:1340	ΔH° > 0, ΔS° > 0, and ∆G° < 0 indicated that AZT removal was spontaneous and endothermic in nature.					
36347864	3	27	from	process	496:502	arg1	effect					444:449	The effect	440:449	The effect of various key factors in the AZT adsorption process	440:502	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	10	28	theme	MIL/Cs	1566:1571	arg1	NCs					1579:1581	MIL/Cs@Fe3O4 NCs	1566:1581	MIL/Cs@Fe3O4 NCs	1566:1581	The performance of MIL/Cs@Fe3O4 NCs under real conditions was also tested and promising results were achieved, except in the treatment of AZT from raw wastewater.					
36347864	0	29	theme	Magnetic	0:7	arg1	NH2-MIL-101					9:19	Magnetic NH2-MIL-101	0:19	Magnetic NH2-MIL-101(Al)	0:23	Magnetic NH2-MIL-101(Al)/Chitosan nanocomposite as a novel adsorbent for the removal of azithromycin: modeling and process optimization.					
36347864	6	30	theme	Langmuir	1074:1081	arg1	isotherm					1083:1090	the Langmuir isotherm	1070:1090	the Langmuir isotherm (R2: 0.998, X2: 0.011)	1070:1113	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	10	31	from	treatment	1672:1680	arg1	wastewater					1698:1707	raw wastewater	1694:1707	raw wastewater	1694:1707	The performance of MIL/Cs@Fe3O4 NCs under real conditions was also tested and promising results were achieved, except in the treatment of AZT from raw wastewater.					
36347864	5	32	dep	dose	864:867	arg1	AZT					902:904	AZT	902:904	AZT	902:904	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	5	32	dep	dose	864:867	arg1	10.107 mg/L					921:931	10.107 mg/L	921:931	10.107 mg/L	921:931	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	5	32	dep	dose	864:867	arg1	64.256 min					887:896	64.256 min	887:896	64.256 min	887:896	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	5	32	dep	dose	864:867	arg1	0.279 g/L					870:878	0.279 g/L	870:878	0.279 g/L	870:878	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	2	33	theme	@	362:362	arg1	NCs					369:371	The as-synthesized MIL/Cs@Fe3O4 NCs	337:371	The as-synthesized MIL/Cs@Fe3O4 NCs	337:371	The as-synthesized MIL/Cs@Fe3O4 NCs was characterized by SEM, TEM, XRD, FTIR, BET, and VSM techniques.					
36347864	4	34	theme	parameters	664:673	arg1	value					618:622	The low value	610:622	The low value of p-value (1.3101e-06) and RSD (1.873) parameters, along with the coefficient of determination > 0.997	610:726	The low value of p-value (1.3101e-06) and RSD (1.873) parameters, along with the coefficient of determination > 0.997 implied that the developed model was well fitted with experimental data.					
36347864	5	35	theme	adsorbent	854:862	arg1	dose					864:867	adsorbent dose	854:867	adsorbent dose	854:867	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	5	35	theme	adsorbent	854:862	arg1	pH					843:844	pH: 7.992	843:851	pH: 7.992	843:851	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	5	35	theme	adsorbent	854:862	arg1	time					881:884	time	881:884	time	881:884	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	1	36	theme	magnetic	163:170	arg1	NH2-MIL-101					172:182	magnetic NH2-MIL-101	163:182	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs)	159:228	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	2	37	theme	MIL/Cs	356:361	arg1	NCs					369:371	The as-synthesized MIL/Cs@Fe3O4 NCs	337:371	The as-synthesized MIL/Cs@Fe3O4 NCs	337:371	The as-synthesized MIL/Cs@Fe3O4 NCs was characterized by SEM, TEM, XRD, FTIR, BET, and VSM techniques.					
36347864	2	38	theme	VSM	424:426	arg1	techniques					428:437	VSM techniques	424:437	VSM techniques	424:437	The as-synthesized MIL/Cs@Fe3O4 NCs was characterized by SEM, TEM, XRD, FTIR, BET, and VSM techniques.					
36347864	6	39	from	monolayer	1209:1217	arg1	nature					1235:1240	nature	1235:1240	nature	1235:1240	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	4	40	theme	low	614:616	arg1	value					618:622	The low value	610:622	The low value of p-value (1.3101e-06) and RSD (1.873) parameters, along with the coefficient of determination > 0.997	610:726	The low value of p-value (1.3101e-06) and RSD (1.873) parameters, along with the coefficient of determination > 0.997 implied that the developed model was well fitted with experimental data.					
36347864	3	41	from	factors	466:472	arg1	process					496:502	the AZT adsorption process	477:502	the AZT adsorption process	477:502	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	1	42	theme	NH2-MIL-101	172:182	arg1	nanocomposite					197:209	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite	159:209	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs)	159:228	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	1	42	theme	NH2-MIL-101	172:182	arg1	NCs					225:227	MIL/Cs@Fe3O4 NCs	212:227	MIL/Cs@Fe3O4 NCs	212:227	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	10	43	theme	raw	1694:1696	arg1	wastewater					1698:1707	raw wastewater	1694:1707	raw wastewater	1694:1707	The performance of MIL/Cs@Fe3O4 NCs under real conditions was also tested and promising results were achieved, except in the treatment of AZT from raw wastewater.					
36347864	4	44	theme	experimental	782:793	arg1	data					795:798	experimental data	782:798	experimental data	782:798	The low value of p-value (1.3101e-06) and RSD (1.873) parameters, along with the coefficient of determination > 0.997 implied that the developed model was well fitted with experimental data.					
36347864	3	45	theme	factors	466:472	arg1	effect					444:449	The effect	440:449	The effect of various key factors in the AZT adsorption process	440:502	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	7	46	theme	AZT	1288:1290	arg1	removal					1292:1298	AZT removal	1288:1298	AZT removal	1288:1298	ΔH° > 0, ΔS° > 0, and ∆G° < 0 indicated that AZT removal was spontaneous and endothermic in nature.					
36347864	6	47	theme	adsorption	1187:1196	arg1	monolayer					1209:1217	monolayer	1209:1217	monolayer	1209:1217	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	6	47	theme	adsorption	1187:1196	arg1	process					1198:1204	the adsorption process	1183:1204	the adsorption process	1183:1204	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	9	48	theme	consecutive	1463:1473	arg1	experiments					1475:1485	10 consecutive experiments	1460:1485	10 consecutive experiments	1460:1485	Reuse of the adsorbent in 10 consecutive experiments showed that removal efficiency was reduced by about 30.24%.					
36347864	5	49	dep	64.256 min	887:896	arg1	concentration					906:918	concentration	906:918	concentration	906:918	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	10	50	theme	@	1572:1572	arg1	NCs					1579:1581	MIL/Cs@Fe3O4 NCs	1566:1581	MIL/Cs@Fe3O4 NCs	1566:1581	The performance of MIL/Cs@Fe3O4 NCs under real conditions was also tested and promising results were achieved, except in the treatment of AZT from raw wastewater.					
36347864	6	51	dep	R2	1149:1150	arg1	0.013					1164:1168	0.013	1164:1168	0.013	1164:1168	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	6	51	dep	R2	1149:1150	arg1	0.999					1153:1157	0.999	1153:1157	0.999	1153:1157	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	5	52	dep	efficiency	942:951	arg1	capacity					972:979	adsorption capacity	961:979	adsorption capacity	961:979	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	4	53	theme	developed	745:753	arg1	model					755:759	the developed model	741:759	the developed model	741:759	The low value of p-value (1.3101e-06) and RSD (1.873) parameters, along with the coefficient of determination > 0.997 implied that the developed model was well fitted with experimental data.					
36347864	1	54	theme	first	325:329	arg1	time					331:334	the first time	321:334	the first time	321:334	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	0	55	dep	/Chitosan	24:32	arg1	process					115:121	process	115:121	process	115:121	Magnetic NH2-MIL-101(Al)/Chitosan nanocomposite as a novel adsorbent for the removal of azithromycin: modeling and process optimization.					
36347864	0	55	dep	/Chitosan	24:32	arg1	modeling					102:109	modeling	102:109	modeling	102:109	Magnetic NH2-MIL-101(Al)/Chitosan nanocomposite as a novel adsorbent for the removal of azithromycin: modeling and process optimization.					
36347864	10	56	theme	real	1589:1592	arg1	conditions					1594:1603	real conditions	1589:1603	real conditions	1589:1603	The performance of MIL/Cs@Fe3O4 NCs under real conditions was also tested and promising results were achieved, except in the treatment of AZT from raw wastewater.					
36347864	6	57	theme	Pseudo-second-order	1119:1137	arg1	kinetics					1139:1146	Pseudo-second-order kinetics	1119:1146	Pseudo-second-order kinetics	1119:1146	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	8	58	theme	Magnesium	1357:1365	arg1	effect					1347:1352	The effect	1343:1352	The effect of Magnesium on AZT adsorption	1343:1383	The effect of Magnesium on AZT adsorption was more complicated than other background ions.					
36347864	8	58	theme	Magnesium	1357:1365	arg1	complicated					1394:1404	complicated	1394:1404	complicated	1394:1404	The effect of Magnesium on AZT adsorption was more complicated than other background ions.					
36347864	9	59	theme	removal	1499:1505	arg1	efficiency					1507:1516	removal efficiency	1499:1516	removal efficiency	1499:1516	Reuse of the adsorbent in 10 consecutive experiments showed that removal efficiency was reduced by about 30.24%.					
36347864	3	60	theme	surface	545:551	arg1	methodology					553:563	response surface methodology	536:563	response surface methodology based on central composite design (RSM-CCD)	536:607	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	3	61	theme	key	462:464	arg1	factors					466:472	various key factors	454:472	various key factors in the AZT adsorption process	454:502	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	4	62	theme	RSD	652:654	arg1	parameters					664:673	p-value (1.3101e-06) and RSD (1.873) parameters	627:673	p-value (1.3101e-06) and RSD (1.873) parameters	627:673	The low value of p-value (1.3101e-06) and RSD (1.873) parameters, along with the coefficient of determination > 0.997 implied that the developed model was well fitted with experimental data.					
36347864	3	63	theme	AZT	481:483	arg1	process					496:502	the AZT adsorption process	477:502	the AZT adsorption process	477:502	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	6	64	from	nature	1235:1240	arg1	monolayer					1209:1217	monolayer	1209:1217	monolayer	1209:1217	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	6	64	from	nature	1235:1240	arg1	process					1198:1204	the adsorption process	1183:1204	the adsorption process	1183:1204	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	3	65	theme	adsorption	485:494	arg1	process					496:502	the AZT adsorption process	477:502	the AZT adsorption process	477:502	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	8	66	theme	background	1417:1426	arg1	ions					1428:1431	other background ions	1411:1431	other background ions	1411:1431	The effect of Magnesium on AZT adsorption was more complicated than other background ions.					
36347864	8	67	theme	AZT	1370:1372	arg1	adsorption					1374:1383	AZT adsorption	1370:1383	AZT adsorption	1370:1383	The effect of Magnesium on AZT adsorption was more complicated than other background ions.					
36347864	6	68	theme	data	1060:1063	arg1	fitting					1049:1055	The fitting	1045:1055	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013)	1045:1169	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	5	69	theme	optimized	811:819	arg1	conditions					821:830	the optimized conditions	807:830	the optimized conditions	807:830	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	5	69	theme	optimized	811:819	arg1	pH					843:844	pH: 7.992	843:851	pH: 7.992	843:851	Under the optimized conditions, including pH: 7.992, adsorbent dose: 0.279 g/L, time: 64.256 min and AZT concentration: 10.107 mg/L, removal efficiency and AZT adsorption capacity were obtained as 98.362 ± 3.24% and 238.553 mg/g, respectively.					
36347864	3	70	from	effect	444:449	arg1	process					496:502	the AZT adsorption process	477:502	the AZT adsorption process	477:502	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	1	71	theme	/chitosan	187:195	arg1	nanocomposite					197:209	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite	159:209	the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs)	159:228	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	1	71	theme	/chitosan	187:195	arg1	NCs					225:227	MIL/Cs@Fe3O4 NCs	212:227	MIL/Cs@Fe3O4 NCs	212:227	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	6	72	dep	isotherm	1083:1090	arg1	R2					1093:1094	R2	1093:1094	R2	1093:1094	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	6	72	dep	isotherm	1083:1090	arg1	X2					1160:1161	X2	1160:1161	X2	1160:1161	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	6	72	dep	isotherm	1083:1090	arg1	X2					1104:1105	X2	1104:1105	X2	1104:1105	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	6	72	dep	isotherm	1083:1090	arg1	R2					1149:1150	R2	1149:1150	R2	1149:1150	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	3	73	theme	response	536:543	arg1	methodology					553:563	response surface methodology	536:563	response surface methodology based on central composite design (RSM-CCD)	536:607	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	0	74	dep	NH2-MIL-101	9:19	arg1	Al					21:22	Al	21:22	Al	21:22	Magnetic NH2-MIL-101(Al)/Chitosan nanocomposite as a novel adsorbent for the removal of azithromycin: modeling and process optimization.					
36347864	2	75	theme	as-synthesized	341:354	arg1	NCs					369:371	The as-synthesized MIL/Cs@Fe3O4 NCs	337:371	The as-synthesized MIL/Cs@Fe3O4 NCs	337:371	The as-synthesized MIL/Cs@Fe3O4 NCs was characterized by SEM, TEM, XRD, FTIR, BET, and VSM techniques.					
36347864	10	76	theme	promising	1625:1633	arg1	results					1635:1641	promising results	1625:1641	promising results	1625:1641	The performance of MIL/Cs@Fe3O4 NCs under real conditions was also tested and promising results were achieved, except in the treatment of AZT from raw wastewater.					
36347864	6	77	with	fitting	1049:1055	arg1	kinetics					1139:1146	Pseudo-second-order kinetics	1119:1146	Pseudo-second-order kinetics	1119:1146	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	6	77	with	fitting	1049:1055	arg1	isotherm					1083:1090	the Langmuir isotherm	1070:1090	the Langmuir isotherm (R2: 0.998, X2: 0.011)	1070:1113	The fitting of data with the Langmuir isotherm (R2: 0.998, X2: 0.011) and Pseudo-second-order kinetics (R2: 0.999, X2: 0.013) showed that the adsorption process is monolayer and chemical in nature.					
36347864	3	78	theme	central	574:580	arg1	RSM-CCD					600:606	RSM-CCD	600:606	RSM-CCD	600:606	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	3	78	theme	central	574:580	arg1	design					592:597	central composite design	574:597	central composite design (RSM-CCD)	574:607	The effect of various key factors in the AZT adsorption process was modeled and optimized using response surface methodology based on central composite design (RSM-CCD).					
36347864	1	79	dep	NH2-MIL-101	172:182	arg1	Al					184:185	Al	184:185	Al	184:185	In the present study, the magnetic NH2-MIL-101(Al)/chitosan nanocomposite (MIL/Cs@Fe3O4 NCs) was synthesized and used in the removal of azithromycin (AZT) from an aqueous solution for the first time.					
36347864	10	80	theme	Fe3O4	1573:1577	arg1	NCs					1579:1581	MIL/Cs@Fe3O4 NCs	1566:1581	MIL/Cs@Fe3O4 NCs	1566:1581	The performance of MIL/Cs@Fe3O4 NCs under real conditions was also tested and promising results were achieved, except in the treatment of AZT from raw wastewater.					
36347864	8	81	theme	other	1411:1415	arg1	ions					1428:1431	other background ions	1411:1431	other background ions	1411:1431	The effect of Magnesium on AZT adsorption was more complicated than other background ions.					
36347864	9	82	from	Reuse	1434:1438	arg1	experiments					1475:1485	10 consecutive experiments	1460:1485	10 consecutive experiments	1460:1485	Reuse of the adsorbent in 10 consecutive experiments showed that removal efficiency was reduced by about 30.24%.					
36600379	0	0	theme	cell	79:82	arg1	walls					84:88	wood cell walls	74:88	wood cell walls	74:88	Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls.					
36600379	1	1	theme	carbon	144:149	arg1	repository					118:127	the most important repository	99:127	the most important repository of assimilated carbon	99:149	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	1	1	theme	carbon	144:149	arg1	Wood					91:94	Wood	91:94	Wood	91:94	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	9	2	theme	complex	1501:1507	arg1	assembly					1509:1516	lignin-carbohydrate complex assembly	1481:1516	lignin-carbohydrate complex assembly	1481:1516	They also suggest an intracellular initiation of lignin-carbohydrate complex assembly.					
36600379	0	3	theme	wood	74:77	arg1	walls					84:88	wood cell walls	74:88	wood cell walls	74:88	Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls.					
36600379	7	4	from	contrast	1084:1091	arg1	insensitive					1138:1148	insensitive	1138:1148	insensitive	1138:1148	In contrast, γ-ester bonds between mGlcA and lignin were insensitive to cell wall-localized α-glucuronidase, supporting the intracellular formation of these bonds.					
36600379	5	5	contain	having	830:835	arg2	composition					837:847	composition	837:847	composition similar to suberin, which required mGlcA for binding to cell walls	837:914	The enzyme affected the content of aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls.					
36600379	5	5	contain	having	830:835	arg1	components					819:828	aliphatic insoluble cell wall components	789:828	aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls	789:914	The enzyme affected the content of aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls.					
36600379	6	6	theme	softwood	1063:1070	arg1	species					1072:1078	both hardwood and softwood species	1045:1078	species	1072:1078	Such suberin-like compounds have been previously identified in decayed wood, but here we show their presence in healthy wood of both hardwood and softwood species.					
36600379	1	7	theme	soluble	315:321	arg1	compounds					368:376	phenolic and aliphatic compounds	345:376	phenolic and aliphatic compounds	345:376	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	1	7	theme	soluble	315:321	arg1	extractives					323:333	soluble extractives	315:333	soluble extractives including phenolic and aliphatic compounds	315:376	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	8	8	theme	mGlcA	1352:1356	arg1	substituent					1358:1368	mGlcA substituent	1352:1368	mGlcA substituent of xylan	1352:1377	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	5	9	theme	similar	849:855	arg1	composition					837:847	composition	837:847	composition similar to suberin, which required mGlcA for binding to cell walls	837:914	The enzyme affected the content of aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls.					
36600379	4	10	theme	substituent	700:710	arg1	contents					642:649	contents	642:649	contents of the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan	642:719	and arabidopsis (Arabidopsis thaliana (L.) Heynh], to decrease contents of the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan, to elucidate mGlcA's functions.					
36600379	6	11	theme	Such	917:920	arg1	compounds					935:943	Such suberin-like compounds	917:943	Such suberin-like compounds	917:943	Such suberin-like compounds have been previously identified in decayed wood, but here we show their presence in healthy wood of both hardwood and softwood species.					
36600379	6	12	attach	presence	1017:1024	arg2	species					1072:1078	both hardwood and softwood species	1045:1078	species	1072:1078	Such suberin-like compounds have been previously identified in decayed wood, but here we show their presence in healthy wood of both hardwood and softwood species.					
36600379	6	12	attach	presence	1017:1024	arg1	wood					1037:1040	healthy wood	1029:1040	healthy wood	1029:1040	Such suberin-like compounds have been previously identified in decayed wood, but here we show their presence in healthy wood of both hardwood and softwood species.					
36600379	3	13	theme	aspen	531:535	arg1	Michx					571:575	aspen [Populus tremula L. x tremuloides Michx	531:575	aspen [Populus tremula L. x tremuloides Michx	531:575	We have targeted expression of a fungal α-glucuronidase to the wood cell wall of aspen [Populus tremula L. x tremuloides Michx.)					
36600379	3	14	theme	α-glucuronidase	490:504	arg1	expression					467:476	expression	467:476	expression of a fungal α-glucuronidase	467:504	We have targeted expression of a fungal α-glucuronidase to the wood cell wall of aspen [Populus tremula L. x tremuloides Michx.)					
36600379	4	15	theme	acid	687:690	arg1	substituent					700:710	the 4-O-methyl glucuronopyranose acid (mGlcA) substituent	654:710	the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan	654:719	and arabidopsis (Arabidopsis thaliana (L.) Heynh], to decrease contents of the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan, to elucidate mGlcA's functions.					
36600379	6	16	theme	species	1072:1078	arg1	presence					1017:1024	their presence	1011:1024	their presence in healthy wood of both hardwood and softwood species	1011:1078	Such suberin-like compounds have been previously identified in decayed wood, but here we show their presence in healthy wood of both hardwood and softwood species.					
36600379	6	17	from	presence	1017:1024	arg1	wood					1037:1040	healthy wood	1029:1040	healthy wood	1029:1040	Such suberin-like compounds have been previously identified in decayed wood, but here we show their presence in healthy wood of both hardwood and softwood species.					
36600379	3	18	theme	cell	518:521	arg1	wall					523:526	the wood cell wall	509:526	the wood cell wall of aspen [Populus tremula L. x tremuloides Michx	509:575	We have targeted expression of a fungal α-glucuronidase to the wood cell wall of aspen [Populus tremula L. x tremuloides Michx.)					
36600379	2	19	theme	Molecular	379:387	arg1	interactions					389:400	Molecular interactions	379:400	Molecular interactions among these compounds	379:422	Molecular interactions among these compounds are not fully understood.					
36600379	8	20	theme	current	1274:1280	arg1	view					1282:1285	the current view	1270:1285	the current view of the wood cell wall composition	1270:1319	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	1	21	theme	phenolic	345:352	arg1	compounds					368:376	phenolic and aliphatic compounds	345:376	phenolic and aliphatic compounds	345:376	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	8	22	theme	cell	1299:1302	arg1	composition					1309:1319	the wood cell wall composition	1290:1319	the wood cell wall composition	1290:1319	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	6	23	theme	healthy	1029:1035	arg1	wood					1037:1040	healthy wood	1029:1040	healthy wood	1029:1040	Such suberin-like compounds have been previously identified in decayed wood, but here we show their presence in healthy wood of both hardwood and softwood species.					
36600379	5	24	theme	wall	814:817	arg1	components					819:828	aliphatic insoluble cell wall components	789:828	aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls	789:914	The enzyme affected the content of aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls.					
36600379	0	25	theme	glucuronic	6:15	arg1	chains					27:32	Xylan glucuronic acid side chains	0:32	Xylan glucuronic acid side chains	0:32	Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls.					
36600379	7	26	theme	cell	1153:1156	arg1	α-glucuronidase					1173:1187	cell wall-localized α-glucuronidase	1153:1187	cell wall-localized α-glucuronidase	1153:1187	In contrast, γ-ester bonds between mGlcA and lignin were insensitive to cell wall-localized α-glucuronidase, supporting the intracellular formation of these bonds.					
36600379	1	27	from	repository	118:127	arg1	form					176:179	the form	172:179	the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds	172:376	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	1	27	from	repository	118:127	arg1	biosphere					158:166	the biosphere	154:166	the biosphere	154:166	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	3	28	theme	L.	554:555	arg1	Michx					571:575	aspen [Populus tremula L. x tremuloides Michx	531:575	aspen [Populus tremula L. x tremuloides Michx	531:575	We have targeted expression of a fungal α-glucuronidase to the wood cell wall of aspen [Populus tremula L. x tremuloides Michx.)					
36600379	5	29	theme	components	819:828	arg1	content					778:784	the content	774:784	the content of aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls	774:914	The enzyme affected the content of aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls.					
36600379	0	30	theme	Xylan	0:4	arg1	chains					27:32	Xylan glucuronic acid side chains	0:32	Xylan glucuronic acid side chains	0:32	Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls.					
36600379	6	31	theme	suberin-like	922:933	arg1	compounds					935:943	Such suberin-like compounds	917:943	Such suberin-like compounds	917:943	Such suberin-like compounds have been previously identified in decayed wood, but here we show their presence in healthy wood of both hardwood and softwood species.					
36600379	3	32	theme	x	557:557	arg1	Michx					571:575	aspen [Populus tremula L. x tremuloides Michx	531:575	aspen [Populus tremula L. x tremuloides Michx	531:575	We have targeted expression of a fungal α-glucuronidase to the wood cell wall of aspen [Populus tremula L. x tremuloides Michx.)					
36600379	8	33	theme	substituent	1358:1368	arg1	function					1340:1347	a novel function	1332:1347	a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall	1332:1429	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	0	34	theme	side	22:25	arg1	chains					27:32	Xylan glucuronic acid side chains	0:32	Xylan glucuronic acid side chains	0:32	Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls.					
36600379	4	35	theme	mGlcA	693:697	arg1	substituent					700:710	the 4-O-methyl glucuronopyranose acid (mGlcA) substituent	654:710	the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan	654:719	and arabidopsis (Arabidopsis thaliana (L.) Heynh], to decrease contents of the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan, to elucidate mGlcA's functions.					
36600379	8	36	theme	suberin-like	1395:1406	arg1	compounds					1408:1416	suberin-like compounds	1395:1416	suberin-like compounds	1395:1416	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	3	37	theme	[Populus	537:544	arg1	Michx					571:575	aspen [Populus tremula L. x tremuloides Michx	531:575	aspen [Populus tremula L. x tremuloides Michx	531:575	We have targeted expression of a fungal α-glucuronidase to the wood cell wall of aspen [Populus tremula L. x tremuloides Michx.)					
36600379	0	38	theme	acid	17:20	arg1	chains					27:32	Xylan glucuronic acid side chains	0:32	Xylan glucuronic acid side chains	0:32	Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls.					
36600379	8	39	theme	wall	1304:1307	arg1	composition					1309:1319	the wood cell wall composition	1290:1319	the wood cell wall composition	1290:1319	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	1	40	theme	aliphatic	358:366	arg1	compounds					368:376	phenolic and aliphatic compounds	345:376	phenolic and aliphatic compounds	345:376	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	3	41	theme	tremula	546:552	arg1	Michx					571:575	aspen [Populus tremula L. x tremuloides Michx	531:575	aspen [Populus tremula L. x tremuloides Michx	531:575	We have targeted expression of a fungal α-glucuronidase to the wood cell wall of aspen [Populus tremula L. x tremuloides Michx.)					
36600379	9	42	theme	lignin-carbohydrate	1481:1499	arg1	assembly					1509:1516	lignin-carbohydrate complex assembly	1481:1516	lignin-carbohydrate complex assembly	1481:1516	They also suggest an intracellular initiation of lignin-carbohydrate complex assembly.					
36600379	8	43	theme	cell	1421:1424	arg1	wall					1426:1429	cell wall	1421:1429	cell wall	1421:1429	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	4	44	theme	4-O-methyl	658:667	arg1	substituent					700:710	the 4-O-methyl glucuronopyranose acid (mGlcA) substituent	654:710	the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan	654:719	and arabidopsis (Arabidopsis thaliana (L.) Heynh], to decrease contents of the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan, to elucidate mGlcA's functions.					
36600379	7	45	from	insensitive	1138:1148	arg1	contrast					1084:1091	contrast	1084:1091	contrast	1084:1091	In contrast, γ-ester bonds between mGlcA and lignin were insensitive to cell wall-localized α-glucuronidase, supporting the intracellular formation of these bonds.					
36600379	5	46	theme	aliphatic	789:797	arg1	components					819:828	aliphatic insoluble cell wall components	789:828	aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls	789:914	The enzyme affected the content of aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls.					
36600379	5	47	theme	cell	809:812	arg1	components					819:828	aliphatic insoluble cell wall components	789:828	aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls	789:914	The enzyme affected the content of aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls.					
36600379	1	48	dep	polymers	190:197	arg1	cellulose					200:208	cellulose	200:208	cellulose	200:208	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	1	48	dep	polymers	190:197	arg1	polymers					190:197	large polymers	184:197	large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds	184:376	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	1	48	dep	polymers	190:197	arg1	glucuronoxylan					236:249	glucuronoxylan	236:249	glucuronoxylan	236:249	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	1	48	dep	polymers	190:197	arg1	lignin					256:261	lignin	256:261	lignin	256:261	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	1	48	dep	polymers	190:197	arg1	hemicelluloses					211:224	hemicelluloses	211:224	hemicelluloses including glucuronoxylan	211:249	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	7	49	theme	γ-ester	1094:1100	arg1	bonds					1102:1106	γ-ester bonds	1094:1106	γ-ester bonds between mGlcA and lignin	1094:1131	In contrast, γ-ester bonds between mGlcA and lignin were insensitive to cell wall-localized α-glucuronidase, supporting the intracellular formation of these bonds.					
36600379	1	50	theme	large	184:188	arg1	cellulose					200:208	cellulose	200:208	cellulose	200:208	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	1	50	theme	large	184:188	arg1	polymers					190:197	large polymers	184:197	large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds	184:376	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	1	50	theme	large	184:188	arg1	lignin					256:261	lignin	256:261	lignin	256:261	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	0	51	theme	aliphatic	51:59	arg1	compounds					61:69	suberin-like aliphatic compounds	38:69	suberin-like aliphatic compounds	38:69	Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls.					
36600379	8	52	theme	compounds	1408:1416	arg1	fastening					1382:1390	fastening	1382:1390	fastening of suberin-like compounds to cell wall	1382:1429	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	4	53	theme	xylan	715:719	arg1	substituent					700:710	the 4-O-methyl glucuronopyranose acid (mGlcA) substituent	654:710	the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan	654:719	and arabidopsis (Arabidopsis thaliana (L.) Heynh], to decrease contents of the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan, to elucidate mGlcA's functions.					
36600379	3	54	theme	tremuloides	559:569	arg1	Michx					571:575	aspen [Populus tremula L. x tremuloides Michx	531:575	aspen [Populus tremula L. x tremuloides Michx	531:575	We have targeted expression of a fungal α-glucuronidase to the wood cell wall of aspen [Populus tremula L. x tremuloides Michx.)					
36600379	8	55	theme	composition	1309:1319	arg1	view					1282:1285	the current view	1270:1285	the current view of the wood cell wall composition	1270:1319	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	4	56	theme	glucuronopyranose	669:685	arg1	substituent					700:710	the 4-O-methyl glucuronopyranose acid (mGlcA) substituent	654:710	the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan	654:719	and arabidopsis (Arabidopsis thaliana (L.) Heynh], to decrease contents of the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan, to elucidate mGlcA's functions.					
36600379	0	57	theme	suberin-like	38:49	arg1	compounds					61:69	suberin-like aliphatic compounds	38:69	suberin-like aliphatic compounds	38:69	Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls.					
36600379	4	58	dep	Arabidopsis	596:606	arg1	thaliana					608:615	Arabidopsis thaliana	596:615	Arabidopsis thaliana (L.) Heynh	596:626	and arabidopsis (Arabidopsis thaliana (L.) Heynh], to decrease contents of the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan, to elucidate mGlcA's functions.					
36600379	3	59	theme	Michx	571:575	arg1	wall					523:526	the wood cell wall	509:526	the wood cell wall of aspen [Populus tremula L. x tremuloides Michx	509:575	We have targeted expression of a fungal α-glucuronidase to the wood cell wall of aspen [Populus tremula L. x tremuloides Michx.)					
36600379	8	60	theme	xylan	1373:1377	arg1	substituent					1358:1368	mGlcA substituent	1352:1368	mGlcA substituent of xylan	1352:1377	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	3	61	theme	fungal	483:488	arg1	α-glucuronidase					490:504	a fungal α-glucuronidase	481:504	a fungal α-glucuronidase	481:504	We have targeted expression of a fungal α-glucuronidase to the wood cell wall of aspen [Populus tremula L. x tremuloides Michx.)					
36600379	7	62	theme	intracellular	1205:1217	arg1	formation					1219:1227	the intracellular formation	1201:1227	the intracellular formation of these bonds	1201:1242	In contrast, γ-ester bonds between mGlcA and lignin were insensitive to cell wall-localized α-glucuronidase, supporting the intracellular formation of these bonds.					
36600379	8	63	theme	novel	1334:1338	arg1	function					1340:1347	a novel function	1332:1347	a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall	1332:1429	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	1	64	theme	important	108:116	arg1	repository					118:127	the most important repository	99:127	the most important repository of assimilated carbon	99:149	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	1	64	theme	important	108:116	arg1	Wood					91:94	Wood	91:94	Wood	91:94	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	6	65	theme	hardwood	1050:1057	arg1	species					1072:1078	both hardwood and softwood species	1045:1078	species	1072:1078	Such suberin-like compounds have been previously identified in decayed wood, but here we show their presence in healthy wood of both hardwood and softwood species.					
36600379	1	66	theme	polymers	190:197	arg1	form					176:179	the form	172:179	the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds	172:376	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36600379	4	67	dep	arabidopsis	583:593	arg1	L.					618:619	L.	618:619	L.	618:619	and arabidopsis (Arabidopsis thaliana (L.) Heynh], to decrease contents of the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan, to elucidate mGlcA's functions.					
36600379	4	67	dep	arabidopsis	583:593	arg1	Heynh					622:626	Heynh	622:626	Heynh	622:626	and arabidopsis (Arabidopsis thaliana (L.) Heynh], to decrease contents of the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan, to elucidate mGlcA's functions.					
36600379	4	67	dep	arabidopsis	583:593	arg1	Arabidopsis					596:606	Arabidopsis	596:606	Arabidopsis	596:606	and arabidopsis (Arabidopsis thaliana (L.) Heynh], to decrease contents of the 4-O-methyl glucuronopyranose acid (mGlcA) substituent of xylan, to elucidate mGlcA's functions.					
36600379	8	68	theme	wood	1294:1297	arg1	composition					1309:1319	the wood cell wall composition	1290:1319	the wood cell wall composition	1290:1319	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	3	69	theme	wood	513:516	arg1	wall					523:526	the wood cell wall	509:526	the wood cell wall of aspen [Populus tremula L. x tremuloides Michx	509:575	We have targeted expression of a fungal α-glucuronidase to the wood cell wall of aspen [Populus tremula L. x tremuloides Michx.)					
36600379	8	70	from	function	1340:1347	arg1	fastening					1382:1390	fastening	1382:1390	fastening of suberin-like compounds to cell wall	1382:1429	These findings challenge the current view of the wood cell wall composition and reveal a novel function of mGlcA substituent of xylan in fastening of suberin-like compounds to cell wall.					
36600379	7	71	theme	wall-localized	1158:1171	arg1	α-glucuronidase					1173:1187	cell wall-localized α-glucuronidase	1153:1187	cell wall-localized α-glucuronidase	1153:1187	In contrast, γ-ester bonds between mGlcA and lignin were insensitive to cell wall-localized α-glucuronidase, supporting the intracellular formation of these bonds.					
36600379	5	72	theme	insoluble	799:807	arg1	components					819:828	aliphatic insoluble cell wall components	789:828	aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls	789:914	The enzyme affected the content of aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls.					
36600379	9	73	theme	intracellular	1453:1465	arg1	initiation					1467:1476	an intracellular initiation	1450:1476	an intracellular initiation of lignin-carbohydrate complex assembly	1450:1516	They also suggest an intracellular initiation of lignin-carbohydrate complex assembly.					
36600379	5	74	theme	cell	905:908	arg1	walls					910:914	cell walls	905:914	cell walls	905:914	The enzyme affected the content of aliphatic insoluble cell wall components having composition similar to suberin, which required mGlcA for binding to cell walls.					
36600379	9	75	theme	assembly	1509:1516	arg1	initiation					1467:1476	an intracellular initiation	1450:1476	an intracellular initiation of lignin-carbohydrate complex assembly	1450:1516	They also suggest an intracellular initiation of lignin-carbohydrate complex assembly.					
36600379	7	76	theme	bonds	1238:1242	arg1	formation					1219:1227	the intracellular formation	1201:1227	the intracellular formation of these bonds	1201:1242	In contrast, γ-ester bonds between mGlcA and lignin were insensitive to cell wall-localized α-glucuronidase, supporting the intracellular formation of these bonds.					
36600379	1	77	theme	assimilated	132:142	arg1	carbon					144:149	assimilated carbon	132:149	assimilated carbon	132:149	Wood is the most important repository of assimilated carbon in the biosphere, in the form of large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively form a composite, together with soluble extractives including phenolic and aliphatic compounds.					
36646346	0	0	theme	enhanced	88:95	arg1	properties					111:120	enhanced antibacterial properties	88:120	enhanced antibacterial properties	88:120	Chitosan/silk fibroin composite bilayer PCL nanofibrous mats for bone regeneration with enhanced antibacterial properties and improved osteogenic potential.					
36646346	4	1	theme	mats	854:857	arg1	side					841:844	The antibacterial side	823:844	The antibacterial side of PCSP mats (A-layer)	823:867	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	1	2	from	engineering	198:208	arg1	popular					254:260	popular	254:260	popular	254:260	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	1	3	theme	final	271:275	arg1	satisfactory					318:329	satisfactory	318:329	satisfactory	318:329	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	1	3	theme	final	271:275	arg1	outcome					296:302	the final tissue restoration outcome	267:302	the final tissue restoration outcome	267:302	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	4	4	theme	PCSP	849:852	arg1	mats					854:857	PCSP mats	849:857	PCSP mats (A-layer)	849:867	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	4	4	theme	PCSP	849:852	arg1	A-layer					860:866	A-layer	860:866	A-layer	860:866	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	1	5	theme	bone	186:189	arg1	engineering					198:208	bone tissue engineering	186:208	bone tissue engineering	186:208	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	5	6	theme	PCSP	1198:1201	arg1	O-layer					1209:1215	O-layer	1209:1215	O-layer	1209:1215	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	5	6	theme	PCSP	1198:1201	arg1	mats					1203:1206	PCSP mats	1198:1206	PCSP mats (O-layer)	1198:1216	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	1	7	theme	tissue	277:282	arg1	satisfactory					318:329	satisfactory	318:329	satisfactory	318:329	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	1	7	theme	tissue	277:282	arg1	outcome					296:302	the final tissue restoration outcome	267:302	the final tissue restoration outcome	267:302	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	2	8	theme	ε-caprolactone	546:559	arg1	/chitosan					561:569	poly (ε-caprolactone)/chitosan	540:569	poly (ε-caprolactone)/chitosan (referred to as PCL/CS)	540:593	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	3	9	theme	mechanical	707:716	arg1	properties					718:727	mechanical properties	707:727	mechanical properties	707:727	The PCL/CS-SF/PCL (referred to as PCSP) mats exhibited biocompatible properties, sufficient hydrophilicity and mechanical properties, as well as a higher breaking strength (3.6 MPa) than the monolayer of SF/PCL mats (1.5 MPa).					
36646346	4	10	dep	lower	1070:1074	arg1	both					1051:1054	both	1051:1054	both	1051:1054	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	1	11	theme	restoration	284:294	arg1	satisfactory					318:329	satisfactory	318:329	satisfactory	318:329	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	1	11	theme	restoration	284:294	arg1	outcome					296:302	the final tissue restoration outcome	267:302	the final tissue restoration outcome	267:302	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	0	12	theme	antibacterial	97:109	arg1	properties					111:120	enhanced antibacterial properties	88:120	enhanced antibacterial properties	88:120	Chitosan/silk fibroin composite bilayer PCL nanofibrous mats for bone regeneration with enhanced antibacterial properties and improved osteogenic potential.					
36646346	7	13	theme	excellent	1481:1489	arg1	prospects					1491:1499	the excellent prospects	1477:1499	the excellent prospects of applying PCSP mats to bone regeneration with gene delivery	1477:1561	These results indicated the excellent prospects of applying PCSP mats to bone regeneration with gene delivery.					
36646346	0	14	with	regeneration	70:81	arg1	properties					111:120	enhanced antibacterial properties	88:120	enhanced antibacterial properties	88:120	Chitosan/silk fibroin composite bilayer PCL nanofibrous mats for bone regeneration with enhanced antibacterial properties and improved osteogenic potential.					
36646346	0	14	with	regeneration	70:81	arg1	potential					146:154	improved osteogenic potential	126:154	improved osteogenic potential	126:154	Chitosan/silk fibroin composite bilayer PCL nanofibrous mats for bone regeneration with enhanced antibacterial properties and improved osteogenic potential.					
36646346	7	15	theme	PCSP	1513:1516	arg1	mats					1518:1521	PCSP mats	1513:1521	PCSP mats	1513:1521	These results indicated the excellent prospects of applying PCSP mats to bone regeneration with gene delivery.					
36646346	0	16	theme	improved	126:133	arg1	potential					146:154	improved osteogenic potential	126:154	improved osteogenic potential	126:154	Chitosan/silk fibroin composite bilayer PCL nanofibrous mats for bone regeneration with enhanced antibacterial properties and improved osteogenic potential.					
36646346	4	17	theme	antibacterial	827:839	arg1	side					841:844	The antibacterial side	823:844	The antibacterial side of PCSP mats (A-layer)	823:867	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	1	18	from	medicine	173:180	arg1	popular					254:260	popular	254:260	popular	254:260	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	1	19	theme	tissue	191:196	arg1	engineering					198:208	bone tissue engineering	186:208	bone tissue engineering	186:208	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	5	20	theme	nodules	1344:1350	arg1	expression					1300:1309	osteogenesis-related gene expression	1274:1309	osteogenesis-related gene expression	1274:1309	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	5	20	theme	nodules	1344:1350	arg1	formation					1319:1327	the formation	1315:1327	the formation of mineralized nodules	1315:1350	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	7	21	theme	gene	1549:1552	arg1	delivery					1554:1561	gene delivery	1549:1561	gene delivery	1549:1561	These results indicated the excellent prospects of applying PCSP mats to bone regeneration with gene delivery.					
36646346	4	22	theme	survival	922:929	arg1	rate					931:934	the survival rate	918:934	the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %)	918:1044	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	4	22	theme	survival	922:929	arg1	lower					1070:1074	lower	1070:1074	lower	1070:1074	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	2	23	theme	poly	540:543	arg1	/chitosan					561:569	poly (ε-caprolactone)/chitosan	540:569	poly (ε-caprolactone)/chitosan (referred to as PCL/CS)	540:593	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	0	24	theme	fibroin	14:20	arg1	nanofibrous					44:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous	0:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous	0:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous mats for bone regeneration with enhanced antibacterial properties and improved osteogenic potential.					
36646346	3	25	dep	PCL/CS-SF/PCL	600:612	arg1	referred					615:622	referred	615:622	referred to as PCSP	615:633	The PCL/CS-SF/PCL (referred to as PCSP) mats exhibited biocompatible properties, sufficient hydrophilicity and mechanical properties, as well as a higher breaking strength (3.6 MPa) than the monolayer of SF/PCL mats (1.5 MPa).					
36646346	0	26	theme	Chitosan/silk	0:12	arg1	nanofibrous					44:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous	0:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous	0:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous mats for bone regeneration with enhanced antibacterial properties and improved osteogenic potential.					
36646346	5	27	theme	related	1117:1123	arg1	factor					1139:1144	runt related transcription factor 2	1112:1146	runt related transcription factor 2 (Runx2)	1112:1154	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	5	27	theme	related	1117:1123	arg1	Runx2					1149:1153	Runx2	1149:1153	Runx2	1149:1153	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	7	28	with	regeneration	1531:1542	arg1	delivery					1554:1561	gene delivery	1549:1561	gene delivery	1549:1561	These results indicated the excellent prospects of applying PCSP mats to bone regeneration with gene delivery.					
36646346	6	29	theme	mats	1395:1398	arg1	implantation					1368:1379	the implantation	1364:1379	the implantation of PCSP+Runx2 mats	1364:1398	Similarly, the implantation of PCSP+Runx2 mats effectively promoted bone tissue generation in vivo.					
36646346	4	30	dep	aureus	1006:1011	arg1	aureus					1017:1022	S. aureus	1014:1022	S. aureus	1014:1022	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	1	31	theme	various	211:217	arg1	materials					229:237	various composite materials	211:237	various composite materials	211:237	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	0	32	theme	bilayer	32:38	arg1	nanofibrous					44:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous	0:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous	0:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous mats for bone regeneration with enhanced antibacterial properties and improved osteogenic potential.					
36646346	5	33	theme	transcription	1125:1137	arg1	factor					1139:1144	runt related transcription factor 2	1112:1146	runt related transcription factor 2 (Runx2)	1112:1154	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	5	33	theme	transcription	1125:1137	arg1	Runx2					1149:1153	Runx2	1149:1153	Runx2	1149:1153	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	4	34	dep	coli	951:954	arg1	coli					960:963	E. coli	957:963	E. coli	957:963	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	1	35	theme	composite	219:227	arg1	materials					229:237	various composite materials	211:237	various composite materials	211:237	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	0	36	theme	composite	22:30	arg1	nanofibrous					44:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous	0:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous	0:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous mats for bone regeneration with enhanced antibacterial properties and improved osteogenic potential.					
36646346	3	37	theme	higher	743:748	arg1	strength					759:766	a higher breaking strength	741:766	a higher breaking strength (3.6 MPa)	741:776	The PCL/CS-SF/PCL (referred to as PCSP) mats exhibited biocompatible properties, sufficient hydrophilicity and mechanical properties, as well as a higher breaking strength (3.6 MPa) than the monolayer of SF/PCL mats (1.5 MPa).					
36646346	3	37	theme	higher	743:748	arg1	3.6 MPa					769:775	3.6 MPa	769:775	3.6 MPa	769:775	The PCL/CS-SF/PCL (referred to as PCSP) mats exhibited biocompatible properties, sufficient hydrophilicity and mechanical properties, as well as a higher breaking strength (3.6 MPa) than the monolayer of SF/PCL mats (1.5 MPa).					
36646346	0	38	theme	osteogenic	135:144	arg1	potential					146:154	improved osteogenic potential	126:154	improved osteogenic potential	126:154	Chitosan/silk fibroin composite bilayer PCL nanofibrous mats for bone regeneration with enhanced antibacterial properties and improved osteogenic potential.					
36646346	2	39	theme	fibroin/poly	457:468	arg1	side					444:447	an osteogenic side	430:447	an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL)	430:509	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	2	39	theme	fibroin/poly	457:468	arg1	side					532:535	an antibacterial side	515:535	an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS)	515:593	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	3	40	theme	breaking	750:757	arg1	strength					759:766	a higher breaking strength	741:766	a higher breaking strength (3.6 MPa)	741:776	The PCL/CS-SF/PCL (referred to as PCSP) mats exhibited biocompatible properties, sufficient hydrophilicity and mechanical properties, as well as a higher breaking strength (3.6 MPa) than the monolayer of SF/PCL mats (1.5 MPa).					
36646346	3	40	theme	breaking	750:757	arg1	3.6 MPa					769:775	3.6 MPa	769:775	3.6 MPa	769:775	The PCL/CS-SF/PCL (referred to as PCSP) mats exhibited biocompatible properties, sufficient hydrophilicity and mechanical properties, as well as a higher breaking strength (3.6 MPa) than the monolayer of SF/PCL mats (1.5 MPa).					
36646346	2	41	theme	/chitosan	561:569	arg1	side					444:447	an osteogenic side	430:447	an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL)	430:509	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	2	41	theme	/chitosan	561:569	arg1	side					532:535	an antibacterial side	515:535	an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS)	515:593	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	3	42	theme	biocompatible	651:663	arg1	properties					665:674	biocompatible properties	651:674	biocompatible properties	651:674	The PCL/CS-SF/PCL (referred to as PCSP) mats exhibited biocompatible properties, sufficient hydrophilicity and mechanical properties, as well as a higher breaking strength (3.6 MPa) than the monolayer of SF/PCL mats (1.5 MPa).					
36646346	3	43	theme	PCL/CS-SF/PCL	600:612	arg1	mats					636:639	The PCL/CS-SF/PCL (referred to as PCSP) mats	596:639	The PCL/CS-SF/PCL (referred to as PCSP) mats	596:639	The PCL/CS-SF/PCL (referred to as PCSP) mats exhibited biocompatible properties, sufficient hydrophilicity and mechanical properties, as well as a higher breaking strength (3.6 MPa) than the monolayer of SF/PCL mats (1.5 MPa).					
36646346	6	44	theme	tissue	1426:1431	arg1	generation					1433:1442	bone tissue generation	1421:1442	bone tissue generation	1421:1442	Similarly, the implantation of PCSP+Runx2 mats effectively promoted bone tissue generation in vivo.					
36646346	2	45	theme	silk	452:455	arg1	ε-caprolactone					471:484	ε-caprolactone	471:484	ε-caprolactone	471:484	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	2	45	theme	silk	452:455	arg1	fibroin/poly					457:468	silk fibroin/poly	452:468	silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL)	452:509	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	0	46	theme	PCL	40:42	arg1	nanofibrous					44:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous	0:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous	0:54	Chitosan/silk fibroin composite bilayer PCL nanofibrous mats for bone regeneration with enhanced antibacterial properties and improved osteogenic potential.					
36646346	4	47	theme	aureus	1006:1011	arg1	rate					931:934	the survival rate	918:934	the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %)	918:1044	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	4	47	theme	aureus	1006:1011	arg1	lower					1070:1074	lower	1070:1074	lower	1070:1074	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	2	48	dep	/chitosan	561:569	arg1	referred					572:579	referred	572:579	referred to as PCL/CS	572:592	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	2	49	theme	antibacterial	518:530	arg1	side					532:535	an antibacterial side	515:535	an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS)	515:593	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	4	50	theme	antibacterial	888:900	arg1	potency					902:908	ideal antibacterial potency	882:908	ideal antibacterial potency	882:908	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	2	51	theme	nanofiber	381:389	arg1	mats					391:394	bilayer-deposited multifunctional nanofiber mats	347:394	bilayer-deposited multifunctional nanofiber mats	347:394	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	3	52	theme	sufficient	677:686	arg1	hydrophilicity					688:701	sufficient hydrophilicity	677:701	sufficient hydrophilicity	677:701	The PCL/CS-SF/PCL (referred to as PCSP) mats exhibited biocompatible properties, sufficient hydrophilicity and mechanical properties, as well as a higher breaking strength (3.6 MPa) than the monolayer of SF/PCL mats (1.5 MPa).					
36646346	2	53	dep	fibroin/poly	457:468	arg1	referred					488:495	referred	488:495	referred to as SF/PCL	488:508	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	2	54	theme	multifunctional	365:379	arg1	mats					391:394	bilayer-deposited multifunctional nanofiber mats	347:394	bilayer-deposited multifunctional nanofiber mats	347:394	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	4	55	theme	coli	951:954	arg1	rate					931:934	the survival rate	918:934	the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %)	918:1044	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	4	55	theme	coli	951:954	arg1	lower					1070:1074	lower	1070:1074	lower	1070:1074	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	5	56	theme	runt	1112:1115	arg1	factor					1139:1144	runt related transcription factor 2	1112:1146	runt related transcription factor 2 (Runx2)	1112:1154	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	5	56	theme	runt	1112:1115	arg1	Runx2					1149:1153	Runx2	1149:1153	Runx2	1149:1153	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	1	57	from	popular	254:260	arg1	medicine					173:180	regenerative medicine	160:180	regenerative medicine	160:180	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	1	57	from	popular	254:260	arg1	engineering					198:208	bone tissue engineering	186:208	bone tissue engineering	186:208	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	5	58	theme	mineralized	1332:1342	arg1	nodules					1344:1350	mineralized nodules	1332:1350	mineralized nodules	1332:1350	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	2	59	theme	bilayer-deposited	347:363	arg1	mats					391:394	bilayer-deposited multifunctional nanofiber mats	347:394	bilayer-deposited multifunctional nanofiber mats	347:394	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	3	60	theme	SF/PCL	800:805	arg1	1.5 MPa					813:819	1.5 MPa	813:819	1.5 MPa	813:819	The PCL/CS-SF/PCL (referred to as PCSP) mats exhibited biocompatible properties, sufficient hydrophilicity and mechanical properties, as well as a higher breaking strength (3.6 MPa) than the monolayer of SF/PCL mats (1.5 MPa).					
36646346	3	60	theme	SF/PCL	800:805	arg1	mats					807:810	SF/PCL mats	800:810	SF/PCL mats (1.5 MPa)	800:820	The PCL/CS-SF/PCL (referred to as PCSP) mats exhibited biocompatible properties, sufficient hydrophilicity and mechanical properties, as well as a higher breaking strength (3.6 MPa) than the monolayer of SF/PCL mats (1.5 MPa).					
36646346	4	61	theme	ideal	882:886	arg1	potency					902:908	ideal antibacterial potency	882:908	ideal antibacterial potency	882:908	The antibacterial side of PCSP mats (A-layer) demonstrated ideal antibacterial potency because the survival rate of Escherichia coli (E. coli) (approximately 25 %) and Staphylococcus aureus (S. aureus) (approximately 15 %) were both significantly lower.					
36646346	5	62	theme	osteogenesis-related	1274:1293	arg1	expression					1300:1309	osteogenesis-related gene expression	1274:1309	osteogenesis-related gene expression	1274:1309	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	3	63	theme	mats	807:810	arg1	monolayer					787:795	the monolayer	783:795	the monolayer of SF/PCL mats (1.5 MPa)	783:820	The PCL/CS-SF/PCL (referred to as PCSP) mats exhibited biocompatible properties, sufficient hydrophilicity and mechanical properties, as well as a higher breaking strength (3.6 MPa) than the monolayer of SF/PCL mats (1.5 MPa).					
36646346	5	64	theme	mats	1203:1206	arg1	side					1190:1193	the osteogenic side	1175:1193	the osteogenic side of PCSP mats (O-layer)	1175:1216	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	5	65	theme	gene	1295:1298	arg1	expression					1300:1309	osteogenesis-related gene expression	1274:1309	osteogenesis-related gene expression	1274:1309	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
36646346	1	66	theme	regenerative	160:171	arg1	medicine					173:180	regenerative medicine	160:180	regenerative medicine	160:180	In regenerative medicine and bone tissue engineering, various composite materials are enormously popular, but the final tissue restoration outcome is not always satisfactory.					
36646346	2	67	theme	osteogenic	433:442	arg1	side					444:447	an osteogenic side	430:447	an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL)	430:509	In this study, bilayer-deposited multifunctional nanofiber mats were successfully fabricated with an osteogenic side of silk fibroin/poly (ε-caprolactone) (referred to as SF/PCL) and an antibacterial side of poly (ε-caprolactone)/chitosan (referred to as PCL/CS).					
36646346	7	68	theme	bone	1526:1529	arg1	regeneration					1531:1542	bone regeneration	1526:1542	bone regeneration with gene delivery	1526:1561	These results indicated the excellent prospects of applying PCSP mats to bone regeneration with gene delivery.					
36646346	0	69	theme	bone	65:68	arg1	regeneration					70:81	bone regeneration	65:81	bone regeneration with enhanced antibacterial properties and improved osteogenic potential	65:154	Chitosan/silk fibroin composite bilayer PCL nanofibrous mats for bone regeneration with enhanced antibacterial properties and improved osteogenic potential.					
36646346	6	70	theme	PCSP+Runx2	1384:1393	arg1	mats					1395:1398	PCSP+Runx2 mats	1384:1398	PCSP+Runx2 mats	1384:1398	Similarly, the implantation of PCSP+Runx2 mats effectively promoted bone tissue generation in vivo.					
36646346	6	71	theme	bone	1421:1424	arg1	generation					1433:1442	bone tissue generation	1421:1442	bone tissue generation	1421:1442	Similarly, the implantation of PCSP+Runx2 mats effectively promoted bone tissue generation in vivo.					
36646346	5	72	theme	osteogenic	1179:1188	arg1	side					1190:1193	the osteogenic side	1175:1193	the osteogenic side of PCSP mats (O-layer)	1175:1216	Subsequently, the plasmid encoding runt related transcription factor 2 (Runx2) was complexed with the osteogenic side of PCSP mats (O-layer) through polyethyleneimine (PEI), thereby enhancing both osteogenesis-related gene expression and the formation of mineralized nodules.					
35534267	10	0	from	change	1375:1380	arg1	dams					1357:1360	dams	1357:1360	dams	1357:1360	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	14	1	theme	kg	1872:1873	arg1	g					1858:1858	16.6 g	1853:1858	16.6 g of MOLM/100 kg of BW	1853:1879	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	10	2	theme	colostrum	1405:1413	arg1	yield					1415:1419	colostrum yield	1405:1419	colostrum yield	1405:1419	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	1	3	theme	Holstein	211:218	arg1	cows					220:223	Holstein cows	211:223	Holstein cows	211:223	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	14	4	theme	dairy	2086:2090	arg1	cows					2092:2095	dairy cows	2086:2095	dairy cows as late as 5 wk prepartum	2086:2121	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	2	5	theme	treatments	490:499	arg1	one					481:483	one	481:483	one	481:483	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	2	5	theme	treatments	490:499	arg1	treatments					490:499	2 treatments	488:499	2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM)	488:599	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	13	6	dep	peroxidation	1749:1760	arg1	malondialdehyde					1793:1807	malondialdehyde	1793:1807	malondialdehyde	1793:1807	Calves born to MOLM-fed cows were characterized by higher blood glucose whereas lipid peroxidation (nonesterified fatty acids and malondialdehyde) was greatly reduced.					
35534267	13	6	dep	peroxidation	1749:1760	arg1	acids					1783:1787	nonesterified fatty acids	1763:1787	nonesterified fatty acids	1763:1787	Calves born to MOLM-fed cows were characterized by higher blood glucose whereas lipid peroxidation (nonesterified fatty acids and malondialdehyde) was greatly reduced.					
35534267	14	7	theme	IgG	1995:1997	arg1	absorption					1999:2008	higher IgG absorption	1988:2008	higher IgG absorption in newborn calves	1988:2026	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	10	8	from	score	1398:1402	arg1	dams					1357:1360	dams	1357:1360	dams	1357:1360	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	2	9	theme	body	582:585	arg1	MOLM					595:598	MOLM	595:598	MOLM	595:598	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	2	9	theme	body	582:585	arg1	weight					587:592	body weight	582:592	body weight (MOLM)	582:599	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	0	10	theme	Prepartum	0:8	arg1	supplementation					10:24	Prepartum supplementation	0:24	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.	0:145	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	2	11	theme	dry	515:517	arg1	period					519:524	a 6-wk dry period	508:524	a 6-wk dry period	508:524	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	14	12	from	colostrum	1973:1981	arg1	calves					2021:2026	newborn calves	2013:2026	newborn calves	2013:2026	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	2	13	theme	kg	576:577	arg1	g					562:562	16.66 g	556:562	(2) 16.66 g of MOLM/100 kg of body weight (MOLM)	552:599	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	6	14	theme	birth	910:914	arg1	h					905:905	2.5 h	901:905	2.5 h of birth	901:914	Calves received their first colostrum meal within 2.5 h of birth.					
35534267	11	15	theme	MOLM-fed	1473:1480	arg1	cows					1482:1485	MOLM-fed cows	1473:1485	MOLM-fed cows	1473:1485	The colostrum harvested from MOLM-fed cows was higher in composition (fat, protein lactose, and total solids).					
35534267	0	16	theme	quality	97:103	arg1	Effects					57:63	Effects	57:63	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.	0:145	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	5	17	theme	respective	834:843	arg1	dams					845:848	their respective dams	828:848	their respective dams	828:848	At calving, calves were weighed immediately after birth and blood was sampled before receiving colostrum from their respective dams.					
35534267	11	18	theme	total	1540:1544	arg1	solids					1546:1551	total solids	1540:1551	total solids	1540:1551	The colostrum harvested from MOLM-fed cows was higher in composition (fat, protein lactose, and total solids).					
35534267	1	19	theme	Moringa	174:180	arg1	MOLM					202:205	MOLM	202:205	MOLM	202:205	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	1	19	theme	Moringa	174:180	arg1	meal					196:199	supplementing Moringa olifeira leaf meal	160:199	supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows	160:223	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	2	20	dep	treatments	490:499	arg1	g					562:562	16.66 g	556:562	(2) 16.66 g of MOLM/100 kg of body weight (MOLM)	552:599	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	2	20	dep	treatments	490:499	arg1	CON					544:546	CON	544:546	CON	544:546	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	2	20	dep	treatments	490:499	arg1	additive					534:541	additive	534:541	additive	534:541	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	2	21	theme	calving	448:454	arg1	weight					421:426	weight	421:426	weight	421:426	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	2	21	theme	calving	448:454	arg1	day					441:443	expected day	432:443	expected day of calving	432:454	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	10	22	theme	dry	1336:1338	arg1	intake					1347:1352	dry matter intake	1336:1352	dry matter intake in dams	1336:1360	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	9	23	theme	Colostrum	1167:1175	arg1	composition					1177:1187	Colostrum composition	1167:1187	Colostrum composition (protein, fat, lactose, total solids, ash, and metabolizable energy)	1167:1256	Colostrum composition (protein, fat, lactose, total solids, ash, and metabolizable energy) was also determined.					
35534267	1	24	theme	leaf	191:194	arg1	MOLM					202:205	MOLM	202:205	MOLM	202:205	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	1	24	theme	leaf	191:194	arg1	meal					196:199	supplementing Moringa olifeira leaf meal	160:199	supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows	160:223	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	4	25	theme	dry	681:683	arg1	ration					704:709	a dry period total mixed ration	679:709	a dry period total mixed ration (TMR)	679:715	All cows received a dry period total mixed ration (TMR).					
35534267	4	25	theme	dry	681:683	arg1	TMR					712:714	TMR	712:714	TMR	712:714	All cows received a dry period total mixed ration (TMR).					
35534267	0	26	from	Effects	57:63	arg1	health					68:73	health	68:73	health	68:73	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	0	26	from	Effects	57:63	arg1	calf					141:144	the calf	137:144	the calf	137:144	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	14	27	dep	late	2100:2103	arg1	as					2105:2106	as	2105:2106	as	2105:2106	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	1	28	theme	colostrum	327:335	arg1	stage					337:341	the colostrum stage	323:341	the colostrum stage	323:341	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	15	29	theme	dairy	2345:2349	arg1	calf					2351:2354	dairy calf	2345:2354	dairy calf	2345:2354	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
35534267	15	30	with	glucose	2138:2144	arg1	peroxidation					2165:2176	reduced lipid peroxidation	2151:2176	reduced lipid peroxidation	2151:2176	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
35534267	4	31	theme	total	692:696	arg1	ration					704:709	a dry period total mixed ration	679:709	a dry period total mixed ration (TMR)	679:715	All cows received a dry period total mixed ration (TMR).					
35534267	4	31	theme	total	692:696	arg1	TMR					712:714	TMR	712:714	TMR	712:714	All cows received a dry period total mixed ration (TMR).					
35534267	13	32	theme	nonesterified	1763:1775	arg1	acids					1783:1787	nonesterified fatty acids	1763:1787	nonesterified fatty acids	1763:1787	Calves born to MOLM-fed cows were characterized by higher blood glucose whereas lipid peroxidation (nonesterified fatty acids and malondialdehyde) was greatly reduced.					
35534267	1	33	from	Influence	147:155	arg1	quality					252:258	quality	252:258	quality	252:258	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	1	33	from	Influence	147:155	arg1	yield					242:246	(1) colostrum yield	228:246	(1) colostrum yield	228:246	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	7	34	from	colostrum	1001:1009	arg1	feedings					956:963	their first and second feedings	933:963	their first and second feedings of colostrum from the first-harvest colostrum	933:1009	Calves received their first and second feedings of colostrum from the first-harvest colostrum.					
35534267	14	35	theme	colostrum	1941:1949	arg1	yield					1951:1955	colostrum yield	1941:1955	colostrum yield	1941:1955	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	13	36	theme	blood	1721:1725	arg1	glucose					1727:1733	higher blood glucose	1714:1733	higher blood glucose	1714:1733	Calves born to MOLM-fed cows were characterized by higher blood glucose whereas lipid peroxidation (nonesterified fatty acids and malondialdehyde) was greatly reduced.					
35534267	13	37	theme	lipid	1743:1747	arg1	peroxidation					1749:1760	lipid peroxidation	1743:1760	lipid peroxidation (nonesterified fatty acids and malondialdehyde)	1743:1808	Calves born to MOLM-fed cows were characterized by higher blood glucose whereas lipid peroxidation (nonesterified fatty acids and malondialdehyde) was greatly reduced.					
35534267	15	38	theme	increased	2128:2136	arg1	glucose					2138:2144	The increased glucose	2124:2144	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM	2124:2206	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
35534267	14	39	theme	calf	1919:1922	arg1	weight					1930:1935	calf birth weight	1919:1935	calf birth weight	1919:1935	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	7	40	theme	colostrum	968:976	arg1	feedings					956:963	their first and second feedings	933:963	their first and second feedings of colostrum from the first-harvest colostrum	933:1009	Calves received their first and second feedings of colostrum from the first-harvest colostrum.					
35534267	2	41	dep	g	562:562	arg1	2					553:553	2	553:553	2	553:553	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	5	42	from	dams	845:848	arg1	colostrum					813:821	colostrum	813:821	colostrum from their respective dams	813:848	At calving, calves were weighed immediately after birth and blood was sampled before receiving colostrum from their respective dams.					
35534267	1	43	from	absorption	289:298	arg1	calves					309:314	dairy calves	303:314	dairy calves during the colostrum stage	303:341	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	1	44	dep	immunoglobulin	268:281	arg1	2					265:265	2	265:265	2	265:265	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	8	45	theme	fatty	1133:1137	arg1	acids					1139:1143	nonesterified fatty acids	1119:1143	nonesterified fatty acids	1119:1143	Cows' blood was sampled to determine IgG, whereas calves were blood sampled to determine the IgG, glucose, nonesterified fatty acids, and malondialdehyde.					
35534267	2	46	theme	Holstein	391:398	arg1	cows					400:403	Thirty-two pregnant Holstein cows	371:403	Thirty-two pregnant Holstein cows	371:403	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	0	47	from	calf	141:144	arg1	Effects					57:63	Effects	57:63	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.	0:145	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	10	48	theme	weight	1368:1373	arg1	change					1375:1380	body weight change	1363:1380	body weight change	1363:1380	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	0	49	theme	oleifera	37:44	arg1	meal					51:54	Moringa oleifera leaf meal	29:54	Moringa oleifera leaf meal	29:54	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	8	50	dep	sampled	1080:1086	arg1	blood					1074:1078	blood	1074:1078	blood	1074:1078	Cows' blood was sampled to determine IgG, whereas calves were blood sampled to determine the IgG, glucose, nonesterified fatty acids, and malondialdehyde.					
35534267	15	51	from	calf	2351:2354	arg1	days					2329:2332	early days	2323:2332	early days of life in dairy calf	2323:2354	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
35534267	0	52	theme	meal	51:54	arg1	supplementation					10:24	Prepartum supplementation	0:24	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.	0:145	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	10	53	theme	condition	1388:1396	arg1	score					1398:1402	body condition score	1383:1402	body condition score	1383:1402	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	1	54	theme	meal	196:199	arg1	Influence					147:155	Influence	147:155	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage	147:341	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	10	55	theme	calf	1425:1428	arg1	weight					1436:1441	calf birth weight	1425:1441	calf birth weight	1425:1441	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	14	56	theme	5	2108:2108	arg1	wk					2110:2111	wk	2110:2111	wk	2110:2111	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	6	57	theme	first	873:877	arg1	meal					889:892	their first colostrum meal	867:892	their first colostrum meal	867:892	Calves received their first colostrum meal within 2.5 h of birth.					
35534267	0	58	from	quality	97:103	arg1	calf					141:144	the calf	137:144	the calf	137:144	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	1	59	from	quality	252:258	arg1	calves					309:314	dairy calves	303:314	dairy calves during the colostrum stage	303:341	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	14	60	theme	newborn	2013:2019	arg1	calves					2021:2026	newborn calves	2013:2026	newborn calves	2013:2026	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	15	61	used	used	2234:2237	arg2	additive					2249:2256	additive	2249:2256	additive	2249:2256	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
35534267	15	61	used	used	2234:2237	arg2	MOLM					2222:2225	MOLM	2222:2225	MOLM	2222:2225	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
35534267	10	62	theme	study	1298:1302	arg1	results					1283:1289	The results	1279:1289	The results of the study	1279:1302	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	1	63	from	yield	242:246	arg1	calves					309:314	dairy calves	303:314	dairy calves during the colostrum stage	303:341	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	15	64	theme	reduced	2151:2157	arg1	peroxidation					2165:2176	reduced lipid peroxidation	2151:2176	reduced lipid peroxidation	2151:2176	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
35534267	12	65	theme	colostrum	1643:1651	arg1	harvests					1653:1660	all 6 colostrum harvests	1637:1660	all 6 colostrum harvests	1637:1660	Colostrum IgG was greater from cows that received MOLM compared with control from all 6 colostrum harvests.					
35534267	2	66	theme	weight	587:592	arg1	kg					576:577	MOLM/100 kg	567:577	MOLM/100 kg of body weight (MOLM)	567:599	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	14	67	theme	g	1858:1858	arg1	level					1844:1848	the level	1840:1848	the level of 16.6 g of MOLM/100 kg of BW	1840:1879	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	15	68	theme	life	2337:2340	arg1	days					2329:2332	early days	2323:2332	early days of life in dairy calf	2323:2354	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
35534267	14	69	theme	higher	1988:1993	arg1	absorption					1999:2008	higher IgG absorption	1988:2008	higher IgG absorption in newborn calves	1988:2026	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	10	70	theme	birth	1430:1434	arg1	weight					1436:1441	calf birth weight	1425:1441	calf birth weight	1425:1441	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	12	71	from	harvests	1653:1660	arg1	control					1624:1630	control	1624:1630	control from all 6 colostrum harvests	1624:1660	Colostrum IgG was greater from cows that received MOLM compared with control from all 6 colostrum harvests.					
35534267	10	72	from	yield	1415:1419	arg1	dams					1357:1360	dams	1357:1360	dams	1357:1360	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	14	73	from	absorption	1999:2008	arg1	calves					2021:2026	newborn calves	2013:2026	newborn calves	2013:2026	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	2	74	theme	6-wk	510:513	arg1	period					519:524	a 6-wk dry period	508:524	a 6-wk dry period	508:524	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	15	75	theme	early	2323:2327	arg1	days					2329:2332	early days	2323:2332	early days of life in dairy calf	2323:2354	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
35534267	14	76	theme	quality	1965:1971	arg1	colostrum					1973:1981	higher quality colostrum	1958:1981	higher quality colostrum	1958:1981	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	1	77	theme	supplementing	160:172	arg1	MOLM					202:205	MOLM	202:205	MOLM	202:205	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	1	77	theme	supplementing	160:172	arg1	meal					196:199	supplementing Moringa olifeira leaf meal	160:199	supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows	160:223	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	4	78	theme	mixed	698:702	arg1	ration					704:709	a dry period total mixed ration	679:709	a dry period total mixed ration (TMR)	679:715	All cows received a dry period total mixed ration (TMR).					
35534267	4	78	theme	mixed	698:702	arg1	TMR					712:714	TMR	712:714	TMR	712:714	All cows received a dry period total mixed ration (TMR).					
35534267	10	79	theme	matter	1340:1345	arg1	intake					1347:1352	dry matter intake	1336:1352	dry matter intake in dams	1336:1360	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	0	80	theme	acquisition	110:120	arg1	Effects					57:63	Effects	57:63	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.	0:145	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	1	81	theme	dairy	303:307	arg1	calves					309:314	dairy calves	303:314	dairy calves during the colostrum stage	303:341	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	1	82	theme	olifeira	182:189	arg1	MOLM					202:205	MOLM	202:205	MOLM	202:205	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	1	82	theme	olifeira	182:189	arg1	meal					196:199	supplementing Moringa olifeira leaf meal	160:199	supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows	160:223	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	0	83	theme	immunity	125:132	arg1	dam					82:84	the dam	78:84	the dam	78:84	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	0	83	theme	immunity	125:132	arg1	quality					97:103	colostrum quality	87:103	colostrum quality	87:103	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	0	83	theme	immunity	125:132	arg1	acquisition					110:120	acquisition	110:120	acquisition of immunity in the calf	110:144	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	11	84	dep	higher	1491:1496	arg1	lactose					1527:1533	protein lactose	1519:1533	protein lactose	1519:1533	The colostrum harvested from MOLM-fed cows was higher in composition (fat, protein lactose, and total solids).					
35534267	11	84	dep	higher	1491:1496	arg1	solids					1546:1551	total solids	1540:1551	total solids	1540:1551	The colostrum harvested from MOLM-fed cows was higher in composition (fat, protein lactose, and total solids).					
35534267	11	84	dep	higher	1491:1496	arg1	fat					1514:1516	fat	1514:1516	fat	1514:1516	The colostrum harvested from MOLM-fed cows was higher in composition (fat, protein lactose, and total solids).					
35534267	9	85	dep	composition	1177:1187	arg1	energy					1250:1255	metabolizable energy	1236:1255	metabolizable energy	1236:1255	Colostrum composition (protein, fat, lactose, total solids, ash, and metabolizable energy) was also determined.					
35534267	9	85	dep	composition	1177:1187	arg1	protein					1190:1196	protein	1190:1196	protein	1190:1196	Colostrum composition (protein, fat, lactose, total solids, ash, and metabolizable energy) was also determined.					
35534267	9	85	dep	composition	1177:1187	arg1	fat					1199:1201	fat	1199:1201	fat	1199:1201	Colostrum composition (protein, fat, lactose, total solids, ash, and metabolizable energy) was also determined.					
35534267	9	85	dep	composition	1177:1187	arg1	ash					1227:1229	ash	1227:1229	ash	1227:1229	Colostrum composition (protein, fat, lactose, total solids, ash, and metabolizable energy) was also determined.					
35534267	9	85	dep	composition	1177:1187	arg1	lactose					1204:1210	lactose	1204:1210	lactose	1204:1210	Colostrum composition (protein, fat, lactose, total solids, ash, and metabolizable energy) was also determined.					
35534267	9	85	dep	composition	1177:1187	arg1	solids					1219:1224	total solids	1213:1224	total solids	1213:1224	Colostrum composition (protein, fat, lactose, total solids, ash, and metabolizable energy) was also determined.					
35534267	14	86	theme	higher	1958:1963	arg1	colostrum					1973:1981	higher quality colostrum	1958:1981	higher quality colostrum	1958:1981	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	3	87	dep	d	629:629	arg1	to					635:636	to	635:636	to	635:636	Cows were dosed daily from d -35 to 0 relative to calving.					
35534267	2	88	dep	additive	534:541	arg1	1					528:528	1	528:528	1	528:528	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	4	89	theme	period	685:690	arg1	ration					704:709	a dry period total mixed ration	679:709	a dry period total mixed ration (TMR)	679:715	All cows received a dry period total mixed ration (TMR).					
35534267	4	89	theme	period	685:690	arg1	TMR					712:714	TMR	712:714	TMR	712:714	All cows received a dry period total mixed ration (TMR).					
35534267	0	90	from	dam	82:84	arg1	calf					141:144	the calf	137:144	the calf	137:144	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	7	91	theme	first-harvest	987:999	arg1	colostrum					1001:1009	the first-harvest colostrum	983:1009	the first-harvest colostrum	983:1009	Calves received their first and second feedings of colostrum from the first-harvest colostrum.					
35534267	13	92	theme	fatty	1777:1781	arg1	acids					1783:1787	nonesterified fatty acids	1763:1787	nonesterified fatty acids	1763:1787	Calves born to MOLM-fed cows were characterized by higher blood glucose whereas lipid peroxidation (nonesterified fatty acids and malondialdehyde) was greatly reduced.					
35534267	9	93	theme	metabolizable	1236:1248	arg1	energy					1250:1255	metabolizable energy	1236:1255	metabolizable energy	1236:1255	Colostrum composition (protein, fat, lactose, total solids, ash, and metabolizable energy) was also determined.					
35534267	10	94	from	weight	1436:1441	arg1	dams					1357:1360	dams	1357:1360	dams	1357:1360	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	14	95	theme	birth	1924:1928	arg1	weight					1930:1935	calf birth weight	1919:1935	calf birth weight	1919:1935	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	0	96	theme	dam	82:84	arg1	Effects					57:63	Effects	57:63	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.	0:145	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	1	97	theme	immunoglobulin	268:281	arg1	absorption					289:298	(2) immunoglobulin (IgG) absorption	264:298	(2) immunoglobulin (IgG) absorption	264:298	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	2	98	theme	expected	432:439	arg1	day					441:443	expected day	432:443	expected day of calving	432:454	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	10	99	theme	body	1383:1386	arg1	score					1398:1402	body condition score	1383:1402	body condition score	1383:1402	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	0	100	theme	colostrum	87:95	arg1	quality					97:103	colostrum quality	87:103	colostrum quality	87:103	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	12	101	theme	Colostrum	1555:1563	arg1	IgG					1565:1567	Colostrum IgG	1555:1567	Colostrum IgG	1555:1567	Colostrum IgG was greater from cows that received MOLM compared with control from all 6 colostrum harvests.					
35534267	1	102	dep	yield	242:246	arg1	1					229:229	1	229:229	1	229:229	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	14	103	theme	body	1906:1909	arg1	weight					1911:1916	the dam's body weight	1896:1916	the dam's body weight	1896:1916	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	7	104	theme	second	949:954	arg1	feedings					956:963	their first and second feedings	933:963	their first and second feedings of colostrum from the first-harvest colostrum	933:1009	Calves received their first and second feedings of colostrum from the first-harvest colostrum.					
35534267	7	105	theme	first	939:943	arg1	feedings					956:963	their first and second feedings	933:963	their first and second feedings of colostrum from the first-harvest colostrum	933:1009	Calves received their first and second feedings of colostrum from the first-harvest colostrum.					
35534267	13	106	theme	higher	1714:1719	arg1	glucose					1727:1733	higher blood glucose	1714:1733	higher blood glucose	1714:1733	Calves born to MOLM-fed cows were characterized by higher blood glucose whereas lipid peroxidation (nonesterified fatty acids and malondialdehyde) was greatly reduced.					
35534267	15	107	from	days	2329:2332	arg1	calf					2351:2354	dairy calf	2345:2354	dairy calf	2345:2354	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
35534267	0	108	theme	Moringa	29:35	arg1	meal					51:54	Moringa oleifera leaf meal	29:54	Moringa oleifera leaf meal	29:54	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	15	109	from	life	2337:2340	arg1	calf					2351:2354	dairy calf	2345:2354	dairy calf	2345:2354	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
35534267	8	110	theme	nonesterified	1119:1131	arg1	acids					1139:1143	nonesterified fatty acids	1119:1143	nonesterified fatty acids	1119:1143	Cows' blood was sampled to determine IgG, whereas calves were blood sampled to determine the IgG, glucose, nonesterified fatty acids, and malondialdehyde.					
35534267	0	111	theme	leaf	46:49	arg1	meal					51:54	Moringa oleifera leaf meal	29:54	Moringa oleifera leaf meal	29:54	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	1	112	theme	colostrum	232:240	arg1	yield					242:246	(1) colostrum yield	228:246	(1) colostrum yield	228:246	Influence of supplementing Moringa olifeira leaf meal (MOLM) to Holstein cows on (1) colostrum yield and quality and (2) immunoglobulin (IgG) absorption in dairy calves during the colostrum stage was evaluated in the study.					
35534267	9	113	theme	total	1213:1217	arg1	solids					1219:1224	total solids	1213:1224	total solids	1213:1224	Colostrum composition (protein, fat, lactose, total solids, ash, and metabolizable energy) was also determined.					
35534267	2	114	theme	pregnant	382:389	arg1	cows					400:403	Thirty-two pregnant Holstein cows	371:403	Thirty-two pregnant Holstein cows	371:403	Thirty-two pregnant Holstein cows were blocked by weight and expected day of calving and randomly assigned to one of 2 treatments during a 6-wk dry period: (1) no additive (CON) or (2) 16.66 g of MOLM/100 kg of body weight (MOLM).					
35534267	14	115	theme	MOLM	2056:2059	arg1	level					2061:2065	that MOLM level	2051:2065	that MOLM level	2051:2065	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	10	116	theme	body	1363:1366	arg1	change					1375:1380	body weight change	1363:1380	body weight change	1363:1380	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	10	117	from	intake	1347:1352	arg1	dams					1357:1360	dams	1357:1360	dams	1357:1360	The results of the study showed that MOLM did not affect dry matter intake in dams, body weight change, body condition score, colostrum yield, or calf birth weight.					
35534267	13	118	theme	MOLM-fed	1678:1685	arg1	cows					1687:1690	MOLM-fed cows	1678:1690	MOLM-fed cows	1678:1690	Calves born to MOLM-fed cows were characterized by higher blood glucose whereas lipid peroxidation (nonesterified fatty acids and malondialdehyde) was greatly reduced.					
35534267	15	119	theme	maternal	2194:2201	arg1	MOLM					2203:2206	maternal MOLM	2194:2206	maternal MOLM	2194:2206	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
35534267	14	120	theme	wk	2110:2111	arg1	prepartum					2113:2121	5 wk prepartum	2108:2121	dairy cows as late as 5 wk prepartum	2086:2121	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	0	121	from	acquisition	110:120	arg1	calf					141:144	the calf	137:144	the calf	137:144	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	11	122	theme	protein	1519:1525	arg1	lactose					1527:1533	protein lactose	1519:1533	protein lactose	1519:1533	The colostrum harvested from MOLM-fed cows was higher in composition (fat, protein lactose, and total solids).					
35534267	0	123	dep	supplementation	10:24	arg1	Effects					57:63	Effects	57:63	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.	0:145	Prepartum supplementation of Moringa oleifera leaf meal: Effects on health of the dam, colostrum quality, and acquisition of immunity in the calf.					
35534267	6	124	theme	colostrum	879:887	arg1	meal					889:892	their first colostrum meal	867:892	their first colostrum meal	867:892	Calves received their first colostrum meal within 2.5 h of birth.					
35534267	14	125	theme	BW	1878:1879	arg1	kg					1872:1873	MOLM/100 kg	1863:1873	MOLM/100 kg of BW	1863:1879	Although the level of 16.6 g of MOLM/100 kg of BW did not affect the dam's body weight, calf birth weight and colostrum yield, higher quality colostrum, and higher IgG absorption in newborn calves could be achieved when that MOLM level is supplemented to dairy cows as late as 5 wk prepartum.					
35534267	15	126	theme	lipid	2159:2163	arg1	peroxidation					2165:2176	reduced lipid peroxidation	2151:2176	reduced lipid peroxidation	2151:2176	The increased glucose with reduced lipid peroxidation after receiving maternal MOLM suggests that MOLM can be used as a feed additive not only to improve dams' immunity but to also improve health in early days of life in dairy calf.					
36598781	11	0	attach	derived	1603:1609	arg1	myoblasts					1642:1650	human and murine skeletal myoblasts	1616:1650	human and murine skeletal myoblasts	1616:1650	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	11	0	attach	derived	1603:1609	arg2	myotubes					1594:1601	myotubes	1594:1601	myotubes derived from human and murine skeletal myoblasts	1594:1650	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	5	1	theme	collagen	861:868	arg1	fibers					870:875	natural collagen fibers	853:875	natural collagen fibers	853:875	In this range, the fibers structurally match the size and dimensions of natural collagen fibers making them sufficiently large to orient cells.					
36598781	1	2	theme	automated	147:155	arg1	fabrication					157:167	the automated fabrication	143:167	the automated fabrication of complex tissue-mimicking constructs	143:206	For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissue-mimicking constructs, there is a need for developing bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties.					
36598781	11	3	theme	soft	1721:1724	arg1	substrates					1734:1743	soft printed substrates	1721:1743	soft printed substrates with intermediate fiber contents	1721:1776	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	11	4	theme	nonlinear	1694:1702	arg1	architectures					1704:1716	linear and complex nonlinear architectures	1675:1716	linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents	1675:1776	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	11	5	theme	linear	1675:1680	arg1	architectures					1704:1716	linear and complex nonlinear architectures	1675:1716	linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents	1675:1776	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	8	6	link	-derived	1234:1241	arg1	proteins					1243:1250	-derived proteins	1234:1250	-derived proteins	1234:1250	In addition, as the fibers are readily dispersible, composite inks with both carbohydrates and extracellular matrix (ECM)-derived proteins can easily be generated.					
36598781	10	7	theme	tissues	1473:1479	arg1	organization					1446:1457	the spatial organization	1434:1457	the spatial organization of engineered tissues	1434:1479	Interestingly, we find that the spatial organization of engineered tissues can be defined by the shear-induced alignment of fibers during the printing procedure.					
36598781	7	8	with	inks	1047:1050	arg1	properties					1082:1091	excellent shear-thinning properties	1057:1091	excellent shear-thinning properties	1057:1091	By adjusting fiber concentration, 3D printing inks with excellent shear-thinning properties can be established.					
36598781	11	9	theme	skeletal	1633:1640	arg1	myoblasts					1642:1650	human and murine skeletal myoblasts	1616:1650	human and murine skeletal myoblasts	1616:1650	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	12	10	theme	cellulose	1799:1807	arg1	tool					1854:1857	a simple and scalable tool	1832:1857	a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function	1832:1947	Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.					
36598781	12	10	theme	cellulose	1799:1807	arg1	inks					1809:1812	Our nanofibrillated cellulose inks	1779:1812	Our nanofibrillated cellulose inks	1779:1812	Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.					
36598781	12	11	theme	simple	1834:1839	arg1	tool					1854:1857	a simple and scalable tool	1832:1857	a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function	1832:1947	Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.					
36598781	12	11	theme	simple	1834:1839	arg1	inks					1809:1812	Our nanofibrillated cellulose inks	1779:1812	Our nanofibrillated cellulose inks	1779:1812	Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.					
36598781	3	12	theme	cellulose	558:566	arg1	fibrils					568:574	cellulose fibrils	558:574	cellulose fibrils	558:574	Our core methodology relies on tailoring the size and dispersibility of cellulose fibrils through a solvent-controlled partial carboxymethylation.					
36598781	9	13	theme	cross-linkable	1337:1350	arg1	structures					1352:1361	3D printing cell-laden and cross-linkable structures	1310:1361	structures	1352:1361	We apply such composite inks for 3D printing cell-laden and cross-linkable structures, as well as tissue-guiding gel substrates.					
36598781	8	14	dep	carbohydrates	1190:1202	arg1	proteins					1243:1250	-derived proteins	1234:1250	-derived proteins	1234:1250	In addition, as the fibers are readily dispersible, composite inks with both carbohydrates and extracellular matrix (ECM)-derived proteins can easily be generated.					
36598781	8	15	with	inks	1175:1178	arg1	ECM					1230:1232	ECM	1230:1232	ECM	1230:1232	In addition, as the fibers are readily dispersible, composite inks with both carbohydrates and extracellular matrix (ECM)-derived proteins can easily be generated.					
36598781	8	15	with	inks	1175:1178	arg1	carbohydrates					1190:1202	carbohydrates	1190:1202	carbohydrates	1190:1202	In addition, as the fibers are readily dispersible, composite inks with both carbohydrates and extracellular matrix (ECM)-derived proteins can easily be generated.					
36598781	8	15	with	inks	1175:1178	arg1	matrix					1222:1227	extracellular matrix	1208:1227	extracellular matrix (ECM)	1208:1233	In addition, as the fibers are readily dispersible, composite inks with both carbohydrates and extracellular matrix (ECM)-derived proteins can easily be generated.					
36598781	3	16	theme	fibrils	568:574	arg1	dispersibility					540:553	dispersibility	540:553	dispersibility	540:553	Our core methodology relies on tailoring the size and dispersibility of cellulose fibrils through a solvent-controlled partial carboxymethylation.					
36598781	3	16	theme	fibrils	568:574	arg1	size					531:534	size	531:534	size	531:534	Our core methodology relies on tailoring the size and dispersibility of cellulose fibrils through a solvent-controlled partial carboxymethylation.					
36598781	12	17	theme	anisotropic	1875:1885	arg1	tissues					1900:1906	anisotropic human muscle tissues	1875:1906	anisotropic human muscle tissues that mimic native structure and function	1875:1947	Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.					
36598781	4	18	with	lengths	735:741	arg1	diameters					710:718	diameters	710:718	diameters of ∼250 nm	710:729	This way, we generate partially negatively charged cellulose nanofibers with diameters of ∼250 nm and lengths spanning tens to hundreds of microns.					
36598781	11	19	from	architectures	1704:1716	arg1	substrates					1734:1743	soft printed substrates	1721:1743	soft printed substrates with intermediate fiber contents	1721:1776	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	5	20	theme	fibers	870:875	arg1	dimensions					839:848	dimensions	839:848	dimensions	839:848	In this range, the fibers structurally match the size and dimensions of natural collagen fibers making them sufficiently large to orient cells.					
36598781	5	20	theme	fibers	870:875	arg1	size					830:833	size	830:833	size	830:833	In this range, the fibers structurally match the size and dimensions of natural collagen fibers making them sufficiently large to orient cells.					
36598781	1	21	theme	three-dimensional	74:90	arg1	bioprinting					97:107	three-dimensional (3D) bioprinting	74:107	three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissue-mimicking constructs	74:206	For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissue-mimicking constructs, there is a need for developing bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties.					
36598781	0	22	theme	Printing	52:59	arg1	Bioinks					61:67	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks	0:67	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks.	0:68	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks.					
36598781	5	23	dep	size	830:833	arg1	the					826:828	the	826:828	the	826:828	In this range, the fibers structurally match the size and dimensions of natural collagen fibers making them sufficiently large to orient cells.					
36598781	10	24	theme	engineered	1462:1471	arg1	tissues					1473:1479	engineered tissues	1462:1479	engineered tissues	1462:1479	Interestingly, we find that the spatial organization of engineered tissues can be defined by the shear-induced alignment of fibers during the printing procedure.					
36598781	7	25	theme	3D	1035:1036	arg1	inks					1047:1050	3D printing inks	1035:1050	3D printing inks with excellent shear-thinning properties	1035:1091	By adjusting fiber concentration, 3D printing inks with excellent shear-thinning properties can be established.					
36598781	2	26	theme	scalable	384:391	arg1	technique					393:401	a scalable technique	382:401	a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities	382:483	Toward this goal, we here present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities.					
36598781	2	27	theme	unique	450:455	arg1	capabilities					472:483	unique tissue-guiding capabilities	450:483	unique tissue-guiding capabilities	450:483	Toward this goal, we here present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities.					
36598781	11	28	theme	human	1616:1620	arg1	myoblasts					1642:1650	human and murine skeletal myoblasts	1616:1650	human and murine skeletal myoblasts	1616:1650	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	3	29	theme	solvent-controlled	586:603	arg1	carboxymethylation					613:630	a solvent-controlled partial carboxymethylation	584:630	a solvent-controlled partial carboxymethylation	584:630	Our core methodology relies on tailoring the size and dispersibility of cellulose fibrils through a solvent-controlled partial carboxymethylation.					
36598781	11	30	theme	printed	1726:1732	arg1	substrates					1734:1743	soft printed substrates	1721:1743	soft printed substrates with intermediate fiber contents	1721:1776	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	1	31	theme	tissue-mimicking	180:195	arg1	constructs					197:206	complex tissue-mimicking constructs	172:206	complex tissue-mimicking constructs	172:206	For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissue-mimicking constructs, there is a need for developing bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties.					
36598781	0	32	theme	Transparent	0:10	arg1	Bioinks					61:67	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks	0:67	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks.	0:68	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks.					
36598781	8	33	theme	extracellular	1208:1220	arg1	ECM					1230:1232	ECM	1230:1232	ECM	1230:1232	In addition, as the fibers are readily dispersible, composite inks with both carbohydrates and extracellular matrix (ECM)-derived proteins can easily be generated.					
36598781	8	33	theme	extracellular	1208:1220	arg1	matrix					1222:1227	extracellular matrix	1208:1227	extracellular matrix (ECM)	1208:1233	In addition, as the fibers are readily dispersible, composite inks with both carbohydrates and extracellular matrix (ECM)-derived proteins can easily be generated.					
36598781	3	34	theme	partial	605:611	arg1	carboxymethylation					613:630	a solvent-controlled partial carboxymethylation	584:630	a solvent-controlled partial carboxymethylation	584:630	Our core methodology relies on tailoring the size and dispersibility of cellulose fibrils through a solvent-controlled partial carboxymethylation.					
36598781	11	35	with	substrates	1734:1743	arg1	contents					1769:1776	intermediate fiber contents	1750:1776	intermediate fiber contents	1750:1776	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	1	36	theme	constructs	197:206	arg1	fabrication					157:167	the automated fabrication	143:167	the automated fabrication of complex tissue-mimicking constructs	143:206	For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissue-mimicking constructs, there is a need for developing bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties.					
36598781	0	37	theme	Cellulose	29:37	arg1	Bioinks					61:67	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks	0:67	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks.	0:68	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks.					
36598781	4	38	theme	microns	772:778	arg1	hundreds					760:767	hundreds	760:767	hundreds of microns	760:778	This way, we generate partially negatively charged cellulose nanofibers with diameters of ∼250 nm and lengths spanning tens to hundreds of microns.					
36598781	10	39	theme	spatial	1438:1444	arg1	organization					1446:1457	the spatial organization	1434:1457	the spatial organization of engineered tissues	1434:1479	Interestingly, we find that the spatial organization of engineered tissues can be defined by the shear-induced alignment of fibers during the printing procedure.					
36598781	1	40	contain	have	303:306	arg2	properties					336:345	integrated cell-instructive properties	308:345	integrated cell-instructive properties	308:345	For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissue-mimicking constructs, there is a need for developing bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties.					
36598781	1	40	contain	have	303:306	arg1	bioinks					240:246	bioinks	240:246	bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties	240:345	For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissue-mimicking constructs, there is a need for developing bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties.					
36598781	2	41	theme	3D	428:429	arg1	inks					440:443	nanofiber 3D printing inks	418:443	nanofiber 3D printing inks with unique tissue-guiding capabilities	418:483	Toward this goal, we here present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities.					
36598781	2	42	theme	printing	431:438	arg1	inks					440:443	nanofiber 3D printing inks	418:443	nanofiber 3D printing inks with unique tissue-guiding capabilities	418:483	Toward this goal, we here present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities.					
36598781	9	43	theme	tissue-guiding	1375:1388	arg1	substrates					1394:1403	tissue-guiding gel substrates	1375:1403	tissue-guiding gel substrates	1375:1403	We apply such composite inks for 3D printing cell-laden and cross-linkable structures, as well as tissue-guiding gel substrates.					
36598781	12	44	theme	muscle	1893:1898	arg1	tissues					1900:1906	anisotropic human muscle tissues	1875:1906	anisotropic human muscle tissues that mimic native structure and function	1875:1947	Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.					
36598781	11	45	theme	intermediate	1750:1761	arg1	contents					1769:1776	intermediate fiber contents	1750:1776	intermediate fiber contents	1750:1776	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	2	46	theme	nanofiber	418:426	arg1	inks					440:443	nanofiber 3D printing inks	418:443	nanofiber 3D printing inks with unique tissue-guiding capabilities	418:483	Toward this goal, we here present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities.					
36598781	0	47	theme	3D	49:50	arg1	Bioinks					61:67	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks	0:67	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks.	0:68	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks.					
36598781	9	48	theme	gel	1390:1392	arg1	substrates					1394:1403	tissue-guiding gel substrates	1375:1403	tissue-guiding gel substrates	1375:1403	We apply such composite inks for 3D printing cell-laden and cross-linkable structures, as well as tissue-guiding gel substrates.					
36598781	10	49	theme	fibers	1530:1535	arg1	alignment					1517:1525	the shear-induced alignment	1499:1525	the shear-induced alignment of fibers	1499:1535	Interestingly, we find that the spatial organization of engineered tissues can be defined by the shear-induced alignment of fibers during the printing procedure.					
36598781	11	50	theme	fiber	1763:1767	arg1	contents					1769:1776	intermediate fiber contents	1750:1776	intermediate fiber contents	1750:1776	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	0	51	theme	Nanofiber	39:47	arg1	Bioinks					61:67	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks	0:67	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks.	0:68	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks.					
36598781	12	52	theme	native	1919:1924	arg1	structure					1926:1934	native structure	1919:1934	native structure	1919:1934	Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.					
36598781	8	53	theme	-derived	1234:1241	arg1	proteins					1243:1250	-derived proteins	1234:1250	-derived proteins	1234:1250	In addition, as the fibers are readily dispersible, composite inks with both carbohydrates and extracellular matrix (ECM)-derived proteins can easily be generated.					
36598781	2	54	with	inks	440:443	arg1	capabilities					472:483	unique tissue-guiding capabilities	450:483	unique tissue-guiding capabilities	450:483	Toward this goal, we here present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities.					
36598781	4	55	theme	cellulose	684:692	arg1	nanofibers					694:703	partially negatively charged cellulose nanofibers	655:703	partially negatively charged cellulose nanofibers with diameters of ∼250 nm	655:729	This way, we generate partially negatively charged cellulose nanofibers with diameters of ∼250 nm and lengths spanning tens to hundreds of microns.					
36598781	12	56	theme	human	1887:1891	arg1	tissues					1900:1906	anisotropic human muscle tissues	1875:1906	anisotropic human muscle tissues that mimic native structure and function	1875:1947	Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.					
36598781	7	57	theme	excellent	1057:1065	arg1	properties					1082:1091	excellent shear-thinning properties	1057:1091	excellent shear-thinning properties	1057:1091	By adjusting fiber concentration, 3D printing inks with excellent shear-thinning properties can be established.					
36598781	1	58	dep	three-dimensional	74:90	arg1	3D					93:94	3D	93:94	3D	93:94	For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissue-mimicking constructs, there is a need for developing bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties.					
36598781	2	59	theme	tissue-guiding	457:470	arg1	capabilities					472:483	unique tissue-guiding capabilities	450:483	unique tissue-guiding capabilities	450:483	Toward this goal, we here present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities.					
36598781	1	60	theme	integrated	308:317	arg1	properties					336:345	integrated cell-instructive properties	308:345	integrated cell-instructive properties	308:345	For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissue-mimicking constructs, there is a need for developing bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties.					
36598781	4	61	with	nanofibers	694:703	arg1	diameters					710:718	diameters	710:718	diameters of ∼250 nm	710:729	This way, we generate partially negatively charged cellulose nanofibers with diameters of ∼250 nm and lengths spanning tens to hundreds of microns.					
36598781	10	62	theme	shear-induced	1503:1515	arg1	alignment					1517:1525	the shear-induced alignment	1499:1525	the shear-induced alignment of fibers	1499:1535	Interestingly, we find that the spatial organization of engineered tissues can be defined by the shear-induced alignment of fibers during the printing procedure.					
36598781	7	63	theme	printing	1038:1045	arg1	inks					1047:1050	3D printing inks	1035:1050	3D printing inks with excellent shear-thinning properties	1035:1091	By adjusting fiber concentration, 3D printing inks with excellent shear-thinning properties can be established.					
36598781	1	64	theme	cell-instructive	319:334	arg1	properties					336:345	integrated cell-instructive properties	308:345	integrated cell-instructive properties	308:345	For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissue-mimicking constructs, there is a need for developing bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties.					
36598781	7	65	theme	shear-thinning	1067:1080	arg1	properties					1082:1091	excellent shear-thinning properties	1057:1091	excellent shear-thinning properties	1057:1091	By adjusting fiber concentration, 3D printing inks with excellent shear-thinning properties can be established.					
36598781	9	66	theme	composite	1291:1299	arg1	inks					1301:1304	such composite inks	1286:1304	such composite inks for 3D printing cell-laden and cross-linkable structures	1286:1361	We apply such composite inks for 3D printing cell-laden and cross-linkable structures, as well as tissue-guiding gel substrates.					
36598781	11	67	theme	murine	1626:1631	arg1	myoblasts					1642:1650	human and murine skeletal myoblasts	1616:1650	human and murine skeletal myoblasts	1616:1650	Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents.					
36598781	3	68	theme	core	490:493	arg1	methodology					495:505	Our core methodology	486:505	Our core methodology	486:505	Our core methodology relies on tailoring the size and dispersibility of cellulose fibrils through a solvent-controlled partial carboxymethylation.					
36598781	3	69	dep	size	531:534	arg1	the					527:529	the	527:529	the	527:529	Our core methodology relies on tailoring the size and dispersibility of cellulose fibrils through a solvent-controlled partial carboxymethylation.					
36598781	10	70	theme	printing	1548:1555	arg1	procedure					1557:1565	the printing procedure	1544:1565	the printing procedure	1544:1565	Interestingly, we find that the spatial organization of engineered tissues can be defined by the shear-induced alignment of fibers during the printing procedure.					
36598781	5	71	theme	natural	853:859	arg1	fibers					870:875	natural collagen fibers	853:875	natural collagen fibers	853:875	In this range, the fibers structurally match the size and dimensions of natural collagen fibers making them sufficiently large to orient cells.					
36598781	12	72	theme	scalable	1845:1852	arg1	tool					1854:1857	a simple and scalable tool	1832:1857	a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function	1832:1947	Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.					
36598781	12	72	theme	scalable	1845:1852	arg1	inks					1809:1812	Our nanofibrillated cellulose inks	1779:1812	Our nanofibrillated cellulose inks	1779:1812	Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.					
36598781	9	73	theme	such	1286:1289	arg1	inks					1301:1304	such composite inks	1286:1304	such composite inks for 3D printing cell-laden and cross-linkable structures	1286:1361	We apply such composite inks for 3D printing cell-laden and cross-linkable structures, as well as tissue-guiding gel substrates.					
36598781	12	74	theme	nanofibrillated	1783:1797	arg1	tool					1854:1857	a simple and scalable tool	1832:1857	a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function	1832:1947	Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.					
36598781	12	74	theme	nanofibrillated	1783:1797	arg1	inks					1809:1812	Our nanofibrillated cellulose inks	1779:1812	Our nanofibrillated cellulose inks	1779:1812	Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.					
36598781	4	75	theme	charged	676:682	arg1	nanofibers					694:703	partially negatively charged cellulose nanofibers	655:703	partially negatively charged cellulose nanofibers with diameters of ∼250 nm	655:729	This way, we generate partially negatively charged cellulose nanofibers with diameters of ∼250 nm and lengths spanning tens to hundreds of microns.					
36598781	1	76	theme	complex	172:178	arg1	constructs					197:206	complex tissue-mimicking constructs	172:206	complex tissue-mimicking constructs	172:206	For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissue-mimicking constructs, there is a need for developing bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties.					
36598781	8	77	theme	composite	1165:1173	arg1	inks					1175:1178	composite inks	1165:1178	composite inks with both carbohydrates and extracellular matrix (ECM)-derived proteins	1165:1250	In addition, as the fibers are readily dispersible, composite inks with both carbohydrates and extracellular matrix (ECM)-derived proteins can easily be generated.					
36598781	0	78	theme	Cell-Guiding	16:27	arg1	Bioinks					61:67	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks	0:67	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks.	0:68	Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks.					
36598781	7	79	theme	fiber	1014:1018	arg1	concentration					1020:1032	fiber concentration	1014:1032	fiber concentration	1014:1032	By adjusting fiber concentration, 3D printing inks with excellent shear-thinning properties can be established.					
36598781	4	80	theme	nm	728:729	arg1	diameters					710:718	diameters	710:718	diameters of ∼250 nm	710:729	This way, we generate partially negatively charged cellulose nanofibers with diameters of ∼250 nm and lengths spanning tens to hundreds of microns.					
37268230	8	0	theme	optimal	1052:1058	arg1	4					1078:1078	4	1078:1078	4	1078:1078	The optimal adsorption pH was 4.					
37268230	8	0	theme	optimal	1052:1058	arg1	pH					1071:1072	The optimal adsorption pH	1048:1072	The optimal adsorption pH	1048:1072	The optimal adsorption pH was 4.					
37268230	9	1	theme	geopolymer	1188:1197	arg1	matrix					1199:1204	the geopolymer matrix	1184:1204	the geopolymer matrix	1184:1204	Besides pore filling sorption process, the carboxyl and hydroxyl groups from alginate immobilized onto the geopolymer matrix favored the retention of 5-FU ions by hydrogen bonds.					
37268230	2	2	from	samples	447:453	arg1	decontamination					375:389	a promising decontamination	363:389	a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples	363:453	In this work, cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay) were developed for a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples.					
37268230	2	2	from	samples	447:453	arg1	drug					431:434	the 5-fluorouracil (5-FU) cytostatic drug	394:434	the 5-fluorouracil (5-FU) cytostatic drug from water samples	394:453	In this work, cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay) were developed for a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples.					
37268230	3	3	theme	hybrid	512:517	arg1	derivative					519:528	its hybrid derivative	508:528	its hybrid derivative	508:528	The characterization of the prepared geopolymer and its hybrid derivative was performed by scanning electron microscopy, X-ray diffraction, Fourier transform infrared and termogravimetric analysis.					
37268230	11	4	contain	has	1387:1389	arg2	advantages					1432:1441	eco-friendly and cost-effective advantages	1400:1441	not only eco-friendly and cost-effective advantages but also excellent efficiency	1391:1471	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	11	4	contain	has	1387:1389	arg2	efficiency					1462:1471	excellent efficiency	1452:1471	not only eco-friendly and cost-effective advantages but also excellent efficiency	1391:1471	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	11	4	contain	has	1387:1389	arg1	material					1378:1385	this material	1373:1385	this material	1373:1385	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	4	5	theme	dosage	800:805	arg1	adsorbent/water					807:821	a dosage adsorbent/water	798:821	a dosage adsorbent/water of 0.002 g/mL	798:835	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	7	6	theme	maximum	999:1005	arg1	qmax					1028:1031	qmax	1028:1031	qmax	1028:1031	The maximum adsorption capacity (qmax) was 6.2 mg/g.					
37268230	7	6	theme	maximum	999:1005	arg1	6.2 mg/g					1038:1045	6.2 mg/g	1038:1045	6.2 mg/g	1038:1045	The maximum adsorption capacity (qmax) was 6.2 mg/g.					
37268230	7	6	theme	maximum	999:1005	arg1	capacity					1018:1025	The maximum adsorption capacity	995:1025	The maximum adsorption capacity (qmax)	995:1032	The maximum adsorption capacity (qmax) was 6.2 mg/g.					
37268230	3	7	theme	scanning	547:554	arg1	microscopy					565:574	scanning electron microscopy	547:574	scanning electron microscopy	547:574	The characterization of the prepared geopolymer and its hybrid derivative was performed by scanning electron microscopy, X-ray diffraction, Fourier transform infrared and termogravimetric analysis.					
37268230	4	8	theme	alginate/geopolymer	698:716	arg1	AGHB					732:735	AGHB	732:735	AGHB	732:735	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	4	8	theme	alginate/geopolymer	698:716	arg1	beads					725:729	alginate/geopolymer hybrid beads	698:729	alginate/geopolymer hybrid beads (AGHB)	698:736	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	0	9	theme	5-fluorouracil	86:99	arg1	removal					75:81	the removal	71:81	the removal of 5-fluorouracil from contaminated environmental water	71:137	Alginate/geopolymer hybrid beads as an innovative adsorbent applied to the removal of 5-fluorouracil from contaminated environmental water.					
37268230	1	10	contain	has	179:181	arg1	Water					140:144	Water	140:144	Water contaminated by cytostatic drugs	140:177	Water contaminated by cytostatic drugs has many negative impacts on the ecosystems.					
37268230	1	10	contain	has	179:181	arg2	impacts					197:203	many negative impacts	183:203	many negative impacts	183:203	Water contaminated by cytostatic drugs has many negative impacts on the ecosystems.					
37268230	8	11	theme	adsorption	1060:1069	arg1	4					1078:1078	4	1078:1078	4	1078:1078	The optimal adsorption pH was 4.					
37268230	8	11	theme	adsorption	1060:1069	arg1	pH					1071:1072	The optimal adsorption pH	1048:1072	The optimal adsorption pH	1048:1072	The optimal adsorption pH was 4.					
37268230	3	12	theme	electron	556:563	arg1	microscopy					565:574	scanning electron microscopy	547:574	scanning electron microscopy	547:574	The characterization of the prepared geopolymer and its hybrid derivative was performed by scanning electron microscopy, X-ray diffraction, Fourier transform infrared and termogravimetric analysis.					
37268230	9	13	theme	pore	1089:1092	arg1	process					1111:1117	pore filling sorption process	1089:1117	pore filling sorption process	1089:1117	Besides pore filling sorption process, the carboxyl and hydroxyl groups from alginate immobilized onto the geopolymer matrix favored the retention of 5-FU ions by hydrogen bonds.					
37268230	3	14	theme	prepared	484:491	arg1	geopolymer					493:502	the prepared geopolymer	480:502	the prepared geopolymer	480:502	The characterization of the prepared geopolymer and its hybrid derivative was performed by scanning electron microscopy, X-ray diffraction, Fourier transform infrared and termogravimetric analysis.					
37268230	0	15	theme	environmental	119:131	arg1	water					133:137	contaminated environmental water	106:137	contaminated environmental water	106:137	Alginate/geopolymer hybrid beads as an innovative adsorbent applied to the removal of 5-fluorouracil from contaminated environmental water.					
37268230	4	16	theme	excellent	747:755	arg1	efficiency					770:779	an excellent 5-FU removal efficiency	744:779	an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL	744:835	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	4	17	theme	Batch	654:658	arg1	experiments					671:681	Batch adsorption experiments	654:681	Batch adsorption experiments	654:681	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	5	18	theme	isotherms	893:901	arg1	data					903:906	The adsorption isotherms data	878:906	The adsorption isotherms data	878:906	The adsorption isotherms data follow well the Langmuir model.					
37268230	1	19	theme	many	183:186	arg1	impacts					197:203	many negative impacts	183:203	many negative impacts	183:203	Water contaminated by cytostatic drugs has many negative impacts on the ecosystems.					
37268230	0	20	theme	contaminated	106:117	arg1	water					133:137	contaminated environmental water	106:137	contaminated environmental water	106:137	Alginate/geopolymer hybrid beads as an innovative adsorbent applied to the removal of 5-fluorouracil from contaminated environmental water.					
37268230	5	21	theme	Langmuir	924:931	arg1	model					933:937	the Langmuir model	920:937	the Langmuir model	920:937	The adsorption isotherms data follow well the Langmuir model.					
37268230	4	22	theme	5-FU	860:863	arg1	efficiency					770:779	an excellent 5-FU removal efficiency	744:779	an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL	744:835	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	4	22	theme	5-FU	860:863	arg1	concentration					843:855	a concentration	841:855	a concentration of 5-FU of 2.5 mg/L	841:875	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	12	23	contain	have	1587:1590	arg2	application					1600:1610	a great application	1592:1610	a great application	1592:1610	This fact suggests that it could have a great application in the purification of contaminated water.					
37268230	12	23	contain	have	1587:1590	arg1	it					1578:1579	it	1578:1579	it	1578:1579	This fact suggests that it could have a great application in the purification of contaminated water.					
37268230	0	24	theme	Alginate/geopolymer	0:18	arg1	beads					27:31	Alginate/geopolymer hybrid beads	0:31	Alginate/geopolymer hybrid beads as an innovative adsorbent	0:58	Alginate/geopolymer hybrid beads as an innovative adsorbent applied to the removal of 5-fluorouracil from contaminated environmental water.					
37268230	9	25	theme	sorption	1102:1109	arg1	process					1111:1117	pore filling sorption process	1089:1117	pore filling sorption process	1089:1117	Besides pore filling sorption process, the carboxyl and hydroxyl groups from alginate immobilized onto the geopolymer matrix favored the retention of 5-FU ions by hydrogen bonds.					
37268230	1	26	theme	negative	188:195	arg1	impacts					197:203	many negative impacts	183:203	many negative impacts	183:203	Water contaminated by cytostatic drugs has many negative impacts on the ecosystems.					
37268230	0	27	theme	hybrid	20:25	arg1	beads					27:31	Alginate/geopolymer hybrid beads	0:31	Alginate/geopolymer hybrid beads as an innovative adsorbent	0:58	Alginate/geopolymer hybrid beads as an innovative adsorbent applied to the removal of 5-fluorouracil from contaminated environmental water.					
37268230	12	28	theme	contaminated	1635:1646	arg1	water					1648:1652	contaminated water	1635:1652	contaminated water	1635:1652	This fact suggests that it could have a great application in the purification of contaminated water.					
37268230	9	29	theme	5-FU	1231:1234	arg1	ions					1236:1239	5-FU ions	1231:1239	5-FU ions	1231:1239	Besides pore filling sorption process, the carboxyl and hydroxyl groups from alginate immobilized onto the geopolymer matrix favored the retention of 5-FU ions by hydrogen bonds.					
37268230	3	30	theme	X-ray	577:581	arg1	diffraction					583:593	X-ray diffraction	577:593	X-ray diffraction	577:593	The characterization of the prepared geopolymer and its hybrid derivative was performed by scanning electron microscopy, X-ray diffraction, Fourier transform infrared and termogravimetric analysis.					
37268230	10	31	theme	dissolved	1288:1296	arg1	matter					1306:1311	dissolved organic matter	1288:1311	dissolved organic matter	1288:1311	Common competitors, such as dissolved organic matter, do not significantly affected the adsorption.					
37268230	3	32	dep	transform	604:612	arg1	infrared					614:621	infrared	614:621	transform infrared	604:621	The characterization of the prepared geopolymer and its hybrid derivative was performed by scanning electron microscopy, X-ray diffraction, Fourier transform infrared and termogravimetric analysis.					
37268230	2	33	theme	water	441:445	arg1	samples					447:453	water samples	441:453	water samples	441:453	In this work, cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay) were developed for a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples.					
37268230	4	34	theme	5-FU	757:760	arg1	efficiency					770:779	an excellent 5-FU removal efficiency	744:779	an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL	744:835	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	9	35	theme	ions	1236:1239	arg1	retention					1218:1226	the retention	1214:1226	the retention of 5-FU ions	1214:1239	Besides pore filling sorption process, the carboxyl and hydroxyl groups from alginate immobilized onto the geopolymer matrix favored the retention of 5-FU ions by hydrogen bonds.					
37268230	5	36	theme	adsorption	882:891	arg1	data					903:906	The adsorption isotherms data	878:906	The adsorption isotherms data	878:906	The adsorption isotherms data follow well the Langmuir model.					
37268230	9	37	from	alginate	1158:1165	arg1	groups					1146:1151	the carboxyl and hydroxyl groups	1120:1151	groups	1146:1151	Besides pore filling sorption process, the carboxyl and hydroxyl groups from alginate immobilized onto the geopolymer matrix favored the retention of 5-FU ions by hydrogen bonds.					
37268230	4	38	theme	hybrid	718:723	arg1	AGHB					732:735	AGHB	732:735	AGHB	732:735	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	4	38	theme	hybrid	718:723	arg1	beads					725:729	alginate/geopolymer hybrid beads	698:729	alginate/geopolymer hybrid beads (AGHB)	698:736	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	11	39	theme	environmental	1494:1506	arg1	samples					1508:1514	real environmental samples	1489:1514	real environmental samples such as wastewater and surface water	1489:1551	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	11	39	theme	environmental	1494:1506	arg1	wastewater					1524:1533	wastewater	1524:1533	wastewater	1524:1533	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	11	39	theme	environmental	1494:1506	arg1	water					1547:1551	surface water	1539:1551	surface water	1539:1551	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	2	40	theme	cytostatic	420:429	arg1	drug					431:434	the 5-fluorouracil (5-FU) cytostatic drug	394:434	the 5-fluorouracil (5-FU) cytostatic drug from water samples	394:453	In this work, cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay) were developed for a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples.					
37268230	2	41	theme	drug	431:434	arg1	decontamination					375:389	a promising decontamination	363:389	a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples	363:453	In this work, cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay) were developed for a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples.					
37268230	12	42	theme	water	1648:1652	arg1	purification					1619:1630	the purification	1615:1630	the purification of contaminated water	1615:1652	This fact suggests that it could have a great application in the purification of contaminated water.					
37268230	9	43	theme	hydrogen	1244:1251	arg1	bonds					1253:1257	hydrogen bonds	1244:1257	hydrogen bonds	1244:1257	Besides pore filling sorption process, the carboxyl and hydroxyl groups from alginate immobilized onto the geopolymer matrix favored the retention of 5-FU ions by hydrogen bonds.					
37268230	4	44	dep	80	790:791	arg1	to					787:788	to	787:788	to	787:788	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	9	45	theme	carboxyl	1124:1131	arg1	groups					1146:1151	the carboxyl and hydroxyl groups	1120:1151	groups	1146:1151	Besides pore filling sorption process, the carboxyl and hydroxyl groups from alginate immobilized onto the geopolymer matrix favored the retention of 5-FU ions by hydrogen bonds.					
37268230	3	46	theme	derivative	519:528	arg1	characterization					460:475	The characterization	456:475	The characterization of the prepared geopolymer and its hybrid derivative	456:528	The characterization of the prepared geopolymer and its hybrid derivative was performed by scanning electron microscopy, X-ray diffraction, Fourier transform infrared and termogravimetric analysis.					
37268230	9	47	theme	filling	1094:1100	arg1	process					1111:1117	pore filling sorption process	1089:1117	pore filling sorption process	1089:1117	Besides pore filling sorption process, the carboxyl and hydroxyl groups from alginate immobilized onto the geopolymer matrix favored the retention of 5-FU ions by hydrogen bonds.					
37268230	2	48	theme	cross-linked	238:249	arg1	beads					261:265	cross-linked adsorbent beads	238:265	cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay)	238:342	In this work, cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay) were developed for a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples.					
37268230	2	49	theme	5-fluorouracil	398:411	arg1	drug					431:434	the 5-fluorouracil (5-FU) cytostatic drug	394:434	the 5-fluorouracil (5-FU) cytostatic drug from water samples	394:453	In this work, cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay) were developed for a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples.					
37268230	6	50	theme	pseudo-second-order	968:986	arg1	model					988:992	the pseudo-second-order model	964:992	the pseudo-second-order model	964:992	The kinetics data favor the pseudo-second-order model.					
37268230	9	51	theme	hydroxyl	1137:1144	arg1	groups					1146:1151	the carboxyl and hydroxyl groups	1120:1151	groups	1146:1151	Besides pore filling sorption process, the carboxyl and hydroxyl groups from alginate immobilized onto the geopolymer matrix favored the retention of 5-FU ions by hydrogen bonds.					
37268230	11	52	theme	cost-effective	1417:1430	arg1	advantages					1432:1441	eco-friendly and cost-effective advantages	1400:1441	not only eco-friendly and cost-effective advantages but also excellent efficiency	1391:1471	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	12	53	theme	great	1594:1598	arg1	application					1600:1610	a great application	1592:1610	a great application	1592:1610	This fact suggests that it could have a great application in the purification of contaminated water.					
37268230	11	54	theme	real	1489:1492	arg1	samples					1508:1514	real environmental samples	1489:1514	real environmental samples such as wastewater and surface water	1489:1551	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	11	54	theme	real	1489:1492	arg1	wastewater					1524:1533	wastewater	1524:1533	wastewater	1524:1533	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	11	54	theme	real	1489:1492	arg1	water					1547:1551	surface water	1539:1551	surface water	1539:1551	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	2	55	from	decontamination	375:389	arg1	samples					447:453	water samples	441:453	water samples	441:453	In this work, cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay) were developed for a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples.					
37268230	3	56	theme	geopolymer	493:502	arg1	characterization					460:475	The characterization	456:475	The characterization of the prepared geopolymer and its hybrid derivative	456:528	The characterization of the prepared geopolymer and its hybrid derivative was performed by scanning electron microscopy, X-ray diffraction, Fourier transform infrared and termogravimetric analysis.					
37268230	11	57	theme	excellent	1452:1460	arg1	efficiency					1462:1471	excellent efficiency	1452:1471	not only eco-friendly and cost-effective advantages but also excellent efficiency	1391:1471	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	10	58	theme	Common	1260:1265	arg1	matter					1306:1311	dissolved organic matter	1288:1311	dissolved organic matter	1288:1311	Common competitors, such as dissolved organic matter, do not significantly affected the adsorption.					
37268230	10	58	theme	Common	1260:1265	arg1	competitors					1267:1277	Common competitors	1260:1277	Common competitors	1260:1277	Common competitors, such as dissolved organic matter, do not significantly affected the adsorption.					
37268230	3	59	theme	termogravimetric	627:642	arg1	analysis					644:651	termogravimetric analysis	627:651	termogravimetric analysis	627:651	The characterization of the prepared geopolymer and its hybrid derivative was performed by scanning electron microscopy, X-ray diffraction, Fourier transform infrared and termogravimetric analysis.					
37268230	4	60	theme	removal	762:768	arg1	efficiency					770:779	an excellent 5-FU removal efficiency	744:779	an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL	744:835	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	7	61	theme	adsorption	1007:1016	arg1	qmax					1028:1031	qmax	1028:1031	qmax	1028:1031	The maximum adsorption capacity (qmax) was 6.2 mg/g.					
37268230	7	61	theme	adsorption	1007:1016	arg1	6.2 mg/g					1038:1045	6.2 mg/g	1038:1045	6.2 mg/g	1038:1045	The maximum adsorption capacity (qmax) was 6.2 mg/g.					
37268230	7	61	theme	adsorption	1007:1016	arg1	capacity					1018:1025	The maximum adsorption capacity	995:1025	The maximum adsorption capacity (qmax)	995:1032	The maximum adsorption capacity (qmax) was 6.2 mg/g.					
37268230	4	62	theme	%	792:792	arg1	efficiency					770:779	an excellent 5-FU removal efficiency	744:779	an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL	744:835	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	4	62	theme	%	792:792	arg1	concentration					843:855	a concentration	841:855	a concentration of 5-FU of 2.5 mg/L	841:875	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	2	63	theme	illito-kaolinitic	320:336	arg1	clay					338:341	an illito-kaolinitic clay	317:341	an illito-kaolinitic clay	317:341	In this work, cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay) were developed for a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples.					
37268230	10	64	theme	organic	1298:1304	arg1	matter					1306:1311	dissolved organic matter	1288:1311	dissolved organic matter	1288:1311	Common competitors, such as dissolved organic matter, do not significantly affected the adsorption.					
37268230	11	65	theme	surface	1539:1545	arg1	water					1547:1551	surface water	1539:1551	surface water	1539:1551	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	4	66	theme	0.002 g/mL	826:835	arg1	adsorbent/water					807:821	a dosage adsorbent/water	798:821	a dosage adsorbent/water of 0.002 g/mL	798:835	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	4	67	theme	adsorption	660:669	arg1	experiments					671:681	Batch adsorption experiments	654:681	Batch adsorption experiments	654:681	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	2	68	theme	promising	365:373	arg1	decontamination					375:389	a promising decontamination	363:389	a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples	363:453	In this work, cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay) were developed for a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples.					
37268230	3	69	dep	Fourier	596:602	arg1	transform					604:612	transform	604:612	transform infrared	604:621	The characterization of the prepared geopolymer and its hybrid derivative was performed by scanning electron microscopy, X-ray diffraction, Fourier transform infrared and termogravimetric analysis.					
37268230	2	70	link	cross-linked	238:249	arg1	beads					261:265	cross-linked adsorbent beads	238:265	cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay)	238:342	In this work, cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay) were developed for a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples.					
37268230	11	71	theme	eco-friendly	1400:1411	arg1	advantages					1432:1441	eco-friendly and cost-effective advantages	1400:1441	not only eco-friendly and cost-effective advantages but also excellent efficiency	1391:1471	In addition, this material has not only eco-friendly and cost-effective advantages but also excellent efficiency when applied to real environmental samples such as wastewater and surface water.					
37268230	6	72	theme	kinetics	944:951	arg1	data					953:956	The kinetics data	940:956	The kinetics data	940:956	The kinetics data favor the pseudo-second-order model.					
37268230	2	73	theme	adsorbent	251:259	arg1	beads					261:265	cross-linked adsorbent beads	238:265	cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay)	238:342	In this work, cross-linked adsorbent beads based on alginate and a geopolymer (prepared from an illito-kaolinitic clay) were developed for a promising decontamination of the 5-fluorouracil (5-FU) cytostatic drug from water samples.					
37268230	4	74	theme	2.5 mg/L	868:875	arg1	efficiency					770:779	an excellent 5-FU removal efficiency	744:779	an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL	744:835	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	4	74	theme	2.5 mg/L	868:875	arg1	concentration					843:855	a concentration	841:855	a concentration of 5-FU of 2.5 mg/L	841:875	Batch adsorption experiments indicated that alginate/geopolymer hybrid beads (AGHB) allow an excellent 5-FU removal efficiency of up to 80% for a dosage adsorbent/water of 0.002 g/mL and a concentration of 5-FU of 2.5 mg/L.					
37268230	1	75	theme	cytostatic	162:171	arg1	drugs					173:177	cytostatic drugs	162:177	cytostatic drugs	162:177	Water contaminated by cytostatic drugs has many negative impacts on the ecosystems.					
37268230	0	76	from	water	133:137	arg1	removal					75:81	the removal	71:81	the removal of 5-fluorouracil from contaminated environmental water	71:137	Alginate/geopolymer hybrid beads as an innovative adsorbent applied to the removal of 5-fluorouracil from contaminated environmental water.					
35311861	0	0	theme	high-fat/high-sucrose	87:107	arg1	mice					121:124	high-fat/high-sucrose fed C57BL/6 mice	87:124	high-fat/high-sucrose fed C57BL/6 mice	87:124	Pot-pollen supplementation reduces fasting glucose and modulates the gut microbiota in high-fat/high-sucrose fed C57BL/6 mice.					
35311861	1	1	from	flowers	177:183	arg1	pollen					154:159	pollen	154:159	pollen	154:159	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	1	from	flowers	177:183	arg1	pots					282:285	fermented inside cerumen pots	257:285	fermented inside cerumen pots	257:285	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	1	from	flowers	177:183	arg1	mixture					143:149	a mixture	141:149	a mixture	141:149	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	1	from	flowers	177:183	arg1	Pot-pollen					127:136	Pot-pollen	127:136	Pot-pollen	127:136	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	1	from	flowers	177:183	arg1	nectar					165:170	nectar	165:170	nectar	165:170	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	4	2	theme	pot-pollen	640:649	arg1	supplementation					651:665	pot-pollen supplementation	640:665	pot-pollen supplementation	640:665	In HF/HS-fed mice, pot-pollen supplementation decreased fasting blood glucose and increased glucose-stimulated insulin secretion without modifying weight gain, body composition, glucose tolerance, and insulin sensitivity.					
35311861	5	3	from	increase	1006:1013	arg1	abundance					935:943	the abundance	931:943	the abundance of the Rikenellaceae RC9 gut group	931:978	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	5	3	from	increase	1006:1013	arg1	abundance					1022:1030	the abundance	1018:1030	the abundance of Romboutsia	1018:1044	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	5	3	from	increase	1006:1013	arg1	Lactobacillus					984:996	Lactobacillus	984:996	Lactobacillus	984:996	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	5	4	theme	Romboutsia	1035:1044	arg1	abundance					1022:1030	the abundance	1018:1030	the abundance of Romboutsia	1018:1044	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	1	5	theme	nectar	165:170	arg1	pots					282:285	fermented inside cerumen pots	257:285	fermented inside cerumen pots	257:285	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	5	theme	nectar	165:170	arg1	mixture					143:149	a mixture	141:149	a mixture	141:149	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	5	theme	nectar	165:170	arg1	Pot-pollen					127:136	Pot-pollen	127:136	Pot-pollen	127:136	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	2	6	from	polyphenols	313:323	arg1	rich					305:308	rich	305:308	rich	305:308	As pot-pollen is rich in polyphenols, we hypothesized that dietary ingestion could modulate obesity, glucose metabolism, and the gut microbiota in an animal model of diet-induced obesity.					
35311861	1	7	theme	fermented	257:265	arg1	pots					282:285	fermented inside cerumen pots	257:285	fermented inside cerumen pots	257:285	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	7	theme	fermented	257:265	arg1	mixture					143:149	a mixture	141:149	a mixture	141:149	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	7	theme	fermented	257:265	arg1	Pot-pollen					127:136	Pot-pollen	127:136	Pot-pollen	127:136	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	0	8	theme	C57BL/6	113:119	arg1	mice					121:124	high-fat/high-sucrose fed C57BL/6 mice	87:124	high-fat/high-sucrose fed C57BL/6 mice	87:124	Pot-pollen supplementation reduces fasting glucose and modulates the gut microbiota in high-fat/high-sucrose fed C57BL/6 mice.					
35311861	5	9	theme	RC9	966:968	arg1	group					974:978	the Rikenellaceae RC9 gut group	948:978	the Rikenellaceae RC9 gut group	948:978	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	7	10	theme	microbiota	1309:1318	arg1	composition					1320:1330	the gut microbiota composition	1301:1330	the gut microbiota composition	1301:1330	Pot-pollen supplementation-associated changes in the gut microbiota composition correlated with the lowering of fasting glucose levels without modulating weight gain.					
35311861	6	11	theme	positive	1191:1198	arg1	effects					1200:1206	the positive effects	1187:1206	the positive effects of pot-pollen ingestion on fasting glucose	1187:1249	Correlations between genus abundances and metabolic changes in response to supplementation indicated that the gut microbiota contributed to the positive effects of pot-pollen ingestion on fasting glucose.					
35311861	1	12	theme	inside	267:272	arg1	pots					282:285	fermented inside cerumen pots	257:285	fermented inside cerumen pots	257:285	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	12	theme	inside	267:272	arg1	mixture					143:149	a mixture	141:149	a mixture	141:149	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	12	theme	inside	267:272	arg1	Pot-pollen					127:136	Pot-pollen	127:136	Pot-pollen	127:136	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	0	13	theme	fed	109:111	arg1	mice					121:124	high-fat/high-sucrose fed C57BL/6 mice	87:124	high-fat/high-sucrose fed C57BL/6 mice	87:124	Pot-pollen supplementation reduces fasting glucose and modulates the gut microbiota in high-fat/high-sucrose fed C57BL/6 mice.					
35311861	6	14	theme	gut	1157:1159	arg1	microbiota					1161:1170	the gut microbiota	1153:1170	the gut microbiota	1153:1170	Correlations between genus abundances and metabolic changes in response to supplementation indicated that the gut microbiota contributed to the positive effects of pot-pollen ingestion on fasting glucose.					
35311861	4	15	theme	insulin	732:738	arg1	secretion					740:748	glucose-stimulated insulin secretion	713:748	glucose-stimulated insulin secretion	713:748	In HF/HS-fed mice, pot-pollen supplementation decreased fasting blood glucose and increased glucose-stimulated insulin secretion without modifying weight gain, body composition, glucose tolerance, and insulin sensitivity.					
35311861	1	16	theme	cerumen	274:280	arg1	pots					282:285	fermented inside cerumen pots	257:285	fermented inside cerumen pots	257:285	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	16	theme	cerumen	274:280	arg1	mixture					143:149	a mixture	141:149	a mixture	141:149	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	16	theme	cerumen	274:280	arg1	Pot-pollen					127:136	Pot-pollen	127:136	Pot-pollen	127:136	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	5	17	theme	microbiota	895:904	arg1	changes					876:882	changes	876:882	changes	876:882	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	5	17	theme	microbiota	895:904	arg1	decrease					919:926	a decrease	917:926	a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus	917:996	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	5	17	theme	microbiota	895:904	arg1	increase					1006:1013	an increase	1003:1013	an increase in the abundance of Romboutsia	1003:1044	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	6	18	theme	metabolic	1089:1097	arg1	changes					1099:1105	metabolic changes	1089:1105	metabolic changes in response to supplementation	1089:1136	Correlations between genus abundances and metabolic changes in response to supplementation indicated that the gut microbiota contributed to the positive effects of pot-pollen ingestion on fasting glucose.					
35311861	2	19	theme	glucose	389:395	arg1	metabolism					397:406	glucose metabolism	389:406	glucose metabolism	389:406	As pot-pollen is rich in polyphenols, we hypothesized that dietary ingestion could modulate obesity, glucose metabolism, and the gut microbiota in an animal model of diet-induced obesity.					
35311861	2	20	theme	animal	438:443	arg1	model					445:449	an animal model	435:449	an animal model of diet-induced obesity	435:473	As pot-pollen is rich in polyphenols, we hypothesized that dietary ingestion could modulate obesity, glucose metabolism, and the gut microbiota in an animal model of diet-induced obesity.					
35311861	6	21	theme	fasting	1235:1241	arg1	glucose					1243:1249	fasting glucose	1235:1249	fasting glucose	1235:1249	Correlations between genus abundances and metabolic changes in response to supplementation indicated that the gut microbiota contributed to the positive effects of pot-pollen ingestion on fasting glucose.					
35311861	1	22	from	mixture	143:149	arg1	flowers					177:183	flowers	177:183	flowers combined with salivary substances of stingless bees	177:235	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	3	23	theme	HF/HS	542:546	arg1	diet					548:551	a HF/HS diet	540:551	a HF/HS diet	540:551	Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks.					
35311861	7	24	theme	supplementation-associated	1263:1288	arg1	changes					1290:1296	Pot-pollen supplementation-associated changes	1252:1296	Pot-pollen supplementation-associated changes in the gut microbiota composition	1252:1330	Pot-pollen supplementation-associated changes in the gut microbiota composition correlated with the lowering of fasting glucose levels without modulating weight gain.					
35311861	0	25	theme	Pot-pollen	0:9	arg1	supplementation					11:25	Pot-pollen supplementation	0:25	Pot-pollen supplementation	0:25	Pot-pollen supplementation reduces fasting glucose and modulates the gut microbiota in high-fat/high-sucrose fed C57BL/6 mice.					
35311861	4	26	theme	insulin	822:828	arg1	sensitivity					830:840	insulin sensitivity	822:840	insulin sensitivity	822:840	In HF/HS-fed mice, pot-pollen supplementation decreased fasting blood glucose and increased glucose-stimulated insulin secretion without modifying weight gain, body composition, glucose tolerance, and insulin sensitivity.					
35311861	7	27	from	changes	1290:1296	arg1	composition					1320:1330	the gut microbiota composition	1301:1330	the gut microbiota composition	1301:1330	Pot-pollen supplementation-associated changes in the gut microbiota composition correlated with the lowering of fasting glucose levels without modulating weight gain.					
35311861	7	28	theme	glucose	1372:1378	arg1	levels					1380:1385	glucose levels	1372:1385	glucose levels	1372:1385	Pot-pollen supplementation-associated changes in the gut microbiota composition correlated with the lowering of fasting glucose levels without modulating weight gain.					
35311861	5	29	theme	pot-pollen	853:862	arg1	Intake					843:848	Intake	843:848	Intake of pot-pollen	843:862	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	0	30	theme	fasting	35:41	arg1	glucose					43:49	fasting glucose	35:49	fasting glucose	35:49	Pot-pollen supplementation reduces fasting glucose and modulates the gut microbiota in high-fat/high-sucrose fed C57BL/6 mice.					
35311861	5	31	theme	gut	970:972	arg1	group					974:978	the Rikenellaceae RC9 gut group	948:978	the Rikenellaceae RC9 gut group	948:978	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	4	32	theme	glucose-stimulated	713:730	arg1	secretion					740:748	glucose-stimulated insulin secretion	713:748	glucose-stimulated insulin secretion	713:748	In HF/HS-fed mice, pot-pollen supplementation decreased fasting blood glucose and increased glucose-stimulated insulin secretion without modifying weight gain, body composition, glucose tolerance, and insulin sensitivity.					
35311861	4	33	theme	fasting	677:683	arg1	glucose					691:697	fasting blood glucose	677:697	fasting blood glucose	677:697	In HF/HS-fed mice, pot-pollen supplementation decreased fasting blood glucose and increased glucose-stimulated insulin secretion without modifying weight gain, body composition, glucose tolerance, and insulin sensitivity.					
35311861	2	34	theme	gut	417:419	arg1	microbiota					421:430	the gut microbiota	413:430	the gut microbiota	413:430	As pot-pollen is rich in polyphenols, we hypothesized that dietary ingestion could modulate obesity, glucose metabolism, and the gut microbiota in an animal model of diet-induced obesity.					
35311861	2	35	theme	dietary	347:353	arg1	ingestion					355:363	dietary ingestion	347:363	dietary ingestion	347:363	As pot-pollen is rich in polyphenols, we hypothesized that dietary ingestion could modulate obesity, glucose metabolism, and the gut microbiota in an animal model of diet-induced obesity.					
35311861	2	36	theme	obesity	467:473	arg1	model					445:449	an animal model	435:449	an animal model of diet-induced obesity	435:473	As pot-pollen is rich in polyphenols, we hypothesized that dietary ingestion could modulate obesity, glucose metabolism, and the gut microbiota in an animal model of diet-induced obesity.					
35311861	7	37	theme	gut	1305:1307	arg1	composition					1320:1330	the gut microbiota composition	1301:1330	the gut microbiota composition	1301:1330	Pot-pollen supplementation-associated changes in the gut microbiota composition correlated with the lowering of fasting glucose levels without modulating weight gain.					
35311861	3	38	theme	HF/HS	558:562	arg1	diet					564:567	a HF/HS diet	556:567	a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP)	556:605	Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks.					
35311861	3	38	theme	HF/HS	558:562	arg1	HF/HS-PP					597:604	HF/HS-PP	597:604	HF/HS-PP	597:604	Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks.					
35311861	6	39	theme	ingestion	1222:1230	arg1	effects					1200:1206	the positive effects	1187:1206	the positive effects of pot-pollen ingestion on fasting glucose	1187:1249	Correlations between genus abundances and metabolic changes in response to supplementation indicated that the gut microbiota contributed to the positive effects of pot-pollen ingestion on fasting glucose.					
35311861	2	40	theme	diet-induced	454:465	arg1	obesity					467:473	diet-induced obesity	454:473	diet-induced obesity	454:473	As pot-pollen is rich in polyphenols, we hypothesized that dietary ingestion could modulate obesity, glucose metabolism, and the gut microbiota in an animal model of diet-induced obesity.					
35311861	0	41	from	microbiota	73:82	arg1	mice					121:124	high-fat/high-sucrose fed C57BL/6 mice	87:124	high-fat/high-sucrose fed C57BL/6 mice	87:124	Pot-pollen supplementation reduces fasting glucose and modulates the gut microbiota in high-fat/high-sucrose fed C57BL/6 mice.					
35311861	6	42	theme	pot-pollen	1211:1220	arg1	ingestion					1222:1230	pot-pollen ingestion	1211:1230	pot-pollen ingestion	1211:1230	Correlations between genus abundances and metabolic changes in response to supplementation indicated that the gut microbiota contributed to the positive effects of pot-pollen ingestion on fasting glucose.					
35311861	1	43	theme	stingless	222:230	arg1	bees					232:235	stingless bees	222:235	stingless bees	222:235	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	3	44	theme	C57BL/6J	481:488	arg1	mice					490:493	Male C57BL/6J mice	476:493	Male C57BL/6J mice	476:493	Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks.					
35311861	7	45	theme	Pot-pollen	1252:1261	arg1	changes					1290:1296	Pot-pollen supplementation-associated changes	1252:1296	Pot-pollen supplementation-associated changes in the gut microbiota composition	1252:1330	Pot-pollen supplementation-associated changes in the gut microbiota composition correlated with the lowering of fasting glucose levels without modulating weight gain.					
35311861	7	46	theme	weight	1406:1411	arg1	gain					1413:1416	weight gain	1406:1416	weight gain	1406:1416	Pot-pollen supplementation-associated changes in the gut microbiota composition correlated with the lowering of fasting glucose levels without modulating weight gain.					
35311861	4	47	theme	glucose	799:805	arg1	tolerance					807:815	glucose tolerance	799:815	glucose tolerance	799:815	In HF/HS-fed mice, pot-pollen supplementation decreased fasting blood glucose and increased glucose-stimulated insulin secretion without modifying weight gain, body composition, glucose tolerance, and insulin sensitivity.					
35311861	5	48	theme	gut	891:893	arg1	microbiota					895:904	the gut microbiota	887:904	the gut microbiota	887:904	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	5	49	from	decrease	919:926	arg1	abundance					935:943	the abundance	931:943	the abundance of the Rikenellaceae RC9 gut group	931:978	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	5	49	from	decrease	919:926	arg1	abundance					1022:1030	the abundance	1018:1030	the abundance of Romboutsia	1018:1044	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	5	49	from	decrease	919:926	arg1	Lactobacillus					984:996	Lactobacillus	984:996	Lactobacillus	984:996	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	5	50	theme	group	974:978	arg1	abundance					935:943	the abundance	931:943	the abundance of the Rikenellaceae RC9 gut group	931:978	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	5	50	theme	group	974:978	arg1	Lactobacillus					984:996	Lactobacillus	984:996	Lactobacillus	984:996	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	5	51	theme	Rikenellaceae	952:964	arg1	group					974:978	the Rikenellaceae RC9 gut group	948:978	the Rikenellaceae RC9 gut group	948:978	Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia.					
35311861	3	52	theme	%	583:583	arg1	pot-pollen					585:594	0.1% pot-pollen	580:594	0.1% pot-pollen	580:594	Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks.					
35311861	4	53	theme	HF/HS-fed	624:632	arg1	mice					634:637	HF/HS-fed mice	624:637	HF/HS-fed mice	624:637	In HF/HS-fed mice, pot-pollen supplementation decreased fasting blood glucose and increased glucose-stimulated insulin secretion without modifying weight gain, body composition, glucose tolerance, and insulin sensitivity.					
35311861	4	54	theme	blood	685:689	arg1	glucose					691:697	fasting blood glucose	677:697	fasting blood glucose	677:697	In HF/HS-fed mice, pot-pollen supplementation decreased fasting blood glucose and increased glucose-stimulated insulin secretion without modifying weight gain, body composition, glucose tolerance, and insulin sensitivity.					
35311861	6	55	from	effects	1200:1206	arg1	glucose					1243:1249	fasting glucose	1235:1249	fasting glucose	1235:1249	Correlations between genus abundances and metabolic changes in response to supplementation indicated that the gut microbiota contributed to the positive effects of pot-pollen ingestion on fasting glucose.					
35311861	1	56	theme	bees	232:235	arg1	substances					208:217	salivary substances	199:217	salivary substances of stingless bees	199:235	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	6	57	from	changes	1099:1105	arg1	response					1110:1117	response	1110:1117	response to supplementation	1110:1136	Correlations between genus abundances and metabolic changes in response to supplementation indicated that the gut microbiota contributed to the positive effects of pot-pollen ingestion on fasting glucose.					
35311861	4	58	theme	weight	768:773	arg1	gain					775:778	weight gain	768:778	weight gain	768:778	In HF/HS-fed mice, pot-pollen supplementation decreased fasting blood glucose and increased glucose-stimulated insulin secretion without modifying weight gain, body composition, glucose tolerance, and insulin sensitivity.					
35311861	3	59	theme	low-fat/low-sucrose	506:524	arg1	LF/LS					532:536	LF/LS	532:536	LF/LS	532:536	Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks.					
35311861	3	59	theme	low-fat/low-sucrose	506:524	arg1	diet					526:529	a low-fat/low-sucrose diet	504:529	a low-fat/low-sucrose diet (LF/LS)	504:537	Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks.					
35311861	3	60	contain	containing	569:578	arg2	pot-pollen					585:594	0.1% pot-pollen	580:594	0.1% pot-pollen	580:594	Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks.					
35311861	3	60	contain	containing	569:578	arg1	diet					564:567	a HF/HS diet	556:567	a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP)	556:605	Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks.					
35311861	3	60	contain	containing	569:578	arg1	HF/HS-PP					597:604	HF/HS-PP	597:604	HF/HS-PP	597:604	Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks.					
35311861	6	61	theme	genus	1068:1072	arg1	abundances					1074:1083	genus abundances	1068:1083	genus abundances	1068:1083	Correlations between genus abundances and metabolic changes in response to supplementation indicated that the gut microbiota contributed to the positive effects of pot-pollen ingestion on fasting glucose.					
35311861	4	62	theme	body	781:784	arg1	composition					786:796	body composition	781:796	body composition	781:796	In HF/HS-fed mice, pot-pollen supplementation decreased fasting blood glucose and increased glucose-stimulated insulin secretion without modifying weight gain, body composition, glucose tolerance, and insulin sensitivity.					
35311861	1	63	theme	salivary	199:206	arg1	substances					208:217	salivary substances	199:217	salivary substances of stingless bees	199:235	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	3	64	theme	Male	476:479	arg1	mice					490:493	Male C57BL/6J mice	476:493	Male C57BL/6J mice	476:493	Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks.					
35311861	0	65	theme	gut	69:71	arg1	microbiota					73:82	the gut microbiota	65:82	the gut microbiota in high-fat/high-sucrose fed C57BL/6 mice	65:124	Pot-pollen supplementation reduces fasting glucose and modulates the gut microbiota in high-fat/high-sucrose fed C57BL/6 mice.					
35311861	3	66	theme	0.1	580:582	arg1	%					583:583	%	583:583	%	583:583	Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks.					
35311861	6	67	from	abundances	1074:1083	arg1	response					1110:1117	response	1110:1117	response to supplementation	1110:1136	Correlations between genus abundances and metabolic changes in response to supplementation indicated that the gut microbiota contributed to the positive effects of pot-pollen ingestion on fasting glucose.					
35311861	1	68	theme	pollen	154:159	arg1	pots					282:285	fermented inside cerumen pots	257:285	fermented inside cerumen pots	257:285	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	68	theme	pollen	154:159	arg1	mixture					143:149	a mixture	141:149	a mixture	141:149	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	1	68	theme	pollen	154:159	arg1	Pot-pollen					127:136	Pot-pollen	127:136	Pot-pollen	127:136	Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots.					
35311861	2	69	from	rich	305:308	arg1	polyphenols					313:323	polyphenols	313:323	polyphenols	313:323	As pot-pollen is rich in polyphenols, we hypothesized that dietary ingestion could modulate obesity, glucose metabolism, and the gut microbiota in an animal model of diet-induced obesity.					
36521750	7	0	theme	soil	1450:1453	arg1	EC					1455:1456	soil EC	1450:1456	soil EC	1450:1456	Redundancy analysis (RDA) and Spearman correlation analysis showed that these beneficial functional bacteria were mainly positively correlated with soil EC, A-N, and N-N.					
36521750	8	1	theme	Cu	1718:1719	arg1	uptake					1721:1726	Cu uptake	1718:1726	Cu uptake by crops	1718:1735	In summary, this study showed that the technique of combining physically modified carrier materials with pesticides has the potential to reduce Cu contamination in the surrounding agricultural soil during pesticide application, thereby reducing Cu uptake by crops.					
36521750	4	2	theme	different	652:660	arg1	temperatures					662:673	different temperatures	652:673	different temperatures	652:673	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	6	3	theme	HM	1247:1248	arg1	immobilization/tolerance					1251:1274	heavy metal (HM) immobilization/tolerance	1234:1274	heavy metal (HM) immobilization/tolerance	1234:1274	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	2	4	theme	sodium	257:262	arg1	pyrrolidone					302:312	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone	257:312	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)	257:318	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	2	4	theme	sodium	257:262	arg1	PVP					315:317	PVP	315:317	PVP	315:317	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	6	5	theme	metal	1240:1244	arg1	immobilization/tolerance					1251:1274	heavy metal (HM) immobilization/tolerance	1234:1274	heavy metal (HM) immobilization/tolerance	1234:1274	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	1	6	theme	significant	190:200	arg1	risks					209:213	significant health risks	190:213	significant health risks	190:213	Excessive copper (Cu) concentrations pose significant health risks to both plants and humans.					
36521750	6	7	theme	microspheres	1122:1133	arg1	use					1109:1111	the use	1105:1111	the use of blank microspheres	1105:1133	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	4	8	theme	controlled	619:628	arg1	capacities					638:647	variable stimuli-responsive controlled release capacities	591:647	variable stimuli-responsive controlled release capacities	591:647	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	0	9	from	reduction	86:94	arg1	soils					141:145	agricultural soils	128:145	agricultural soils	128:145	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	7	10	theme	beneficial	1380:1389	arg1	bacteria					1402:1409	these beneficial functional bacteria	1374:1409	these beneficial functional bacteria	1374:1409	Redundancy analysis (RDA) and Spearman correlation analysis showed that these beneficial functional bacteria were mainly positively correlated with soil EC, A-N, and N-N.					
36521750	4	11	theme	high	874:877	arg1	temperatures					879:890	high temperatures	874:890	high temperatures	874:890	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	0	12	from	pesticides	71:80	arg1	soils					141:145	agricultural soils	128:145	agricultural soils	128:145	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	8	13	contain	has	1589:1591	arg1	technique					1512:1520	the technique	1508:1520	the technique of combining physically modified carrier materials with pesticides	1508:1587	In summary, this study showed that the technique of combining physically modified carrier materials with pesticides has the potential to reduce Cu contamination in the surrounding agricultural soil during pesticide application, thereby reducing Cu uptake by crops.					
36521750	8	13	contain	has	1589:1591	arg2	potential					1597:1605	the potential to reduce Cu contamination in the surrounding agricultural soil during pesticide application, thereby reducing Cu uptake by crops	1593:1735	the potential to reduce Cu contamination in the surrounding agricultural soil during pesticide application, thereby reducing Cu uptake by crops	1593:1735	In summary, this study showed that the technique of combining physically modified carrier materials with pesticides has the potential to reduce Cu contamination in the surrounding agricultural soil during pesticide application, thereby reducing Cu uptake by crops.					
36521750	6	14	theme	communities	1207:1217	arg1	abundance					1158:1166	the relative abundance	1145:1166	the relative abundance of soil beneficial functional bacteria communities	1145:1217	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	5	15	theme	soil	1039:1042	arg1	content					1053:1059	soil nutrient content	1039:1059	soil nutrient content	1039:1059	In addition, blank microspheres improved the growth and physiological activity of cucumber seedlings, reduced copper content in leaves, increased soil nutrient content, and prevented soil acidification.					
36521750	6	16	theme	soil	1171:1174	arg1	bacteria					1198:1205	soil beneficial functional bacteria	1171:1205	soil beneficial functional bacteria communities	1171:1217	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	5	17	theme	blank	906:910	arg1	microspheres					912:923	blank microspheres	906:923	blank microspheres	906:923	In addition, blank microspheres improved the growth and physiological activity of cucumber seedlings, reduced copper content in leaves, increased soil nutrient content, and prevented soil acidification.					
36521750	7	18	theme	Spearman	1332:1339	arg1	analysis					1353:1360	Spearman correlation analysis	1332:1360	Spearman correlation analysis	1332:1360	Redundancy analysis (RDA) and Spearman correlation analysis showed that these beneficial functional bacteria were mainly positively correlated with soil EC, A-N, and N-N.					
36521750	0	19	theme	pesticides	71:80	arg1	release					60:66	controlled release	49:66	controlled release of pesticides and reduction of adverse effects of copper in agricultural soils	49:145	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	1	20	theme	copper	158:163	arg1	concentrations					170:183	Excessive copper (Cu) concentrations	148:183	Excessive copper (Cu) concentrations	148:183	Excessive copper (Cu) concentrations pose significant health risks to both plants and humans.					
36521750	0	21	theme	reduction	86:94	arg1	release					60:66	controlled release	49:66	controlled release of pesticides and reduction of adverse effects of copper in agricultural soils	49:145	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	0	22	theme	adverse	99:105	arg1	effects					107:113	adverse effects	99:113	adverse effects of copper	99:123	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	5	23	theme	soil	1076:1079	arg1	acidification					1081:1093	soil acidification	1076:1093	soil acidification	1076:1093	In addition, blank microspheres improved the growth and physiological activity of cucumber seedlings, reduced copper content in leaves, increased soil nutrient content, and prevented soil acidification.					
36521750	5	24	theme	physiological	949:961	arg1	activity					963:970	physiological activity	949:970	physiological activity	949:970	In addition, blank microspheres improved the growth and physiological activity of cucumber seedlings, reduced copper content in leaves, increased soil nutrient content, and prevented soil acidification.					
36521750	0	25	from	release	60:66	arg1	soils					141:145	agricultural soils	128:145	agricultural soils	128:145	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	4	26	theme	brevitarsis	761:771	arg1	larvae					741:746	larvae	741:746	larvae of Protaetia brevitarsis	741:771	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	4	27	theme	control	719:725	arg1	efficiency					727:736	better control efficiency	712:736	better control efficiency of larvae of Protaetia brevitarsis	712:771	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	6	28	theme	functional	1187:1196	arg1	bacteria					1198:1205	soil beneficial functional bacteria	1171:1205	soil beneficial functional bacteria communities	1171:1217	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	0	29	theme	composite	22:30	arg1	microspheres					32:43	composite microspheres	22:43	composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils	22:145	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	8	30	theme	modified	1546:1553	arg1	materials					1563:1571	physically modified carrier materials	1535:1571	physically modified carrier materials	1535:1571	In summary, this study showed that the technique of combining physically modified carrier materials with pesticides has the potential to reduce Cu contamination in the surrounding agricultural soil during pesticide application, thereby reducing Cu uptake by crops.					
36521750	4	31	theme	early	805:809	arg1	stage					811:815	the early stage	801:815	the early stage	801:815	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	3	32	theme	loading	410:416	arg1	content					418:424	loading content	410:424	loading content	410:424	The loading content and encapsulation efficiency of optimal microspheres determined by physical modifications were 19.77% and 99.32%, respectively.					
36521750	3	33	theme	optimal	458:464	arg1	microspheres					466:477	optimal microspheres	458:477	optimal microspheres determined by physical modifications	458:514	The loading content and encapsulation efficiency of optimal microspheres determined by physical modifications were 19.77% and 99.32%, respectively.					
36521750	6	34	theme	beneficial	1176:1185	arg1	bacteria					1198:1205	soil beneficial functional bacteria	1171:1205	soil beneficial functional bacteria communities	1171:1217	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	4	35	theme	pesticide	776:784	arg1	application					786:796	pesticide application	776:796	pesticide application in the early stage	776:815	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	2	36	theme	-polyvinyl	291:300	arg1	pyrrolidone					302:312	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone	257:312	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)	257:318	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	2	36	theme	-polyvinyl	291:300	arg1	PVP					315:317	PVP	315:317	PVP	315:317	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	8	37	theme	surrounding	1641:1651	arg1	soil					1666:1669	the surrounding agricultural soil	1637:1669	the surrounding agricultural soil	1637:1669	In summary, this study showed that the technique of combining physically modified carrier materials with pesticides has the potential to reduce Cu contamination in the surrounding agricultural soil during pesticide application, thereby reducing Cu uptake by crops.					
36521750	5	38	theme	seedlings	984:992	arg1	growth					938:943	growth	938:943	growth	938:943	In addition, blank microspheres improved the growth and physiological activity of cucumber seedlings, reduced copper content in leaves, increased soil nutrient content, and prevented soil acidification.					
36521750	5	38	theme	seedlings	984:992	arg1	activity					963:970	physiological activity	949:970	physiological activity	949:970	In addition, blank microspheres improved the growth and physiological activity of cucumber seedlings, reduced copper content in leaves, increased soil nutrient content, and prevented soil acidification.					
36521750	0	39	from	soils	141:145	arg1	release					60:66	controlled release	49:66	controlled release of pesticides and reduction of adverse effects of copper in agricultural soils	49:145	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	3	40	theme	physical	493:500	arg1	modifications					502:514	physical modifications	493:514	physical modifications	493:514	The loading content and encapsulation efficiency of optimal microspheres determined by physical modifications were 19.77% and 99.32%, respectively.					
36521750	6	41	theme	plant	1288:1292	arg1	growth					1294:1299	plant growth	1288:1299	plant growth	1288:1299	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	8	42	theme	pesticide	1678:1686	arg1	application					1688:1698	pesticide application	1678:1698	pesticide application	1678:1698	In summary, this study showed that the technique of combining physically modified carrier materials with pesticides has the potential to reduce Cu contamination in the surrounding agricultural soil during pesticide application, thereby reducing Cu uptake by crops.					
36521750	8	43	theme	Cu	1617:1618	arg1	contamination					1620:1632	Cu contamination	1617:1632	Cu contamination	1617:1632	In summary, this study showed that the technique of combining physically modified carrier materials with pesticides has the potential to reduce Cu contamination in the surrounding agricultural soil during pesticide application, thereby reducing Cu uptake by crops.					
36521750	2	44	theme	SA	274:275	arg1	pyrrolidone					302:312	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone	257:312	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)	257:318	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	2	44	theme	SA	274:275	arg1	PVP					315:317	PVP	315:317	PVP	315:317	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	4	45	theme	pest	856:859	arg1	outbreaks					861:869	pest outbreaks	856:869	pest outbreaks	856:869	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	2	46	theme	alginate	264:271	arg1	pyrrolidone					302:312	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone	257:312	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)	257:318	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	2	46	theme	alginate	264:271	arg1	PVP					315:317	PVP	315:317	PVP	315:317	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	6	47	theme	relative	1149:1156	arg1	abundance					1158:1166	the relative abundance	1145:1166	the relative abundance of soil beneficial functional bacteria communities	1145:1217	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	2	48	theme	GEL	287:289	arg1	pyrrolidone					302:312	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone	257:312	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)	257:318	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	2	48	theme	GEL	287:289	arg1	PVP					315:317	PVP	315:317	PVP	315:317	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	4	49	theme	potential	827:835	arg1	ability					837:843	the potential ability	823:843	the potential ability to control pest outbreaks at high temperatures	823:890	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	6	50	dep	increased	1135:1143	arg1	promote					1280:1286	promote	1280:1286	promote plant growth	1280:1299	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	6	50	dep	increased	1135:1143	arg1	mediate					1226:1232	mediate	1226:1232	mediate heavy metal (HM) immobilization/tolerance	1226:1274	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	0	51	theme	agricultural	128:139	arg1	soils					141:145	agricultural soils	128:145	agricultural soils	128:145	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	2	52	theme	-gelatin	277:284	arg1	pyrrolidone					302:312	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone	257:312	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)	257:318	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	2	52	theme	-gelatin	277:284	arg1	PVP					315:317	PVP	315:317	PVP	315:317	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	1	53	theme	health	202:207	arg1	risks					209:213	significant health risks	190:213	significant health risks	190:213	Excessive copper (Cu) concentrations pose significant health risks to both plants and humans.					
36521750	2	54	theme	embedded	321:328	arg1	DIN					343:345	DIN	343:345	DIN	343:345	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	2	54	theme	embedded	321:328	arg1	dinotefuran					330:340	embedded dinotefuran	321:340	embedded dinotefuran (DIN)	321:346	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	6	55	theme	blank	1116:1120	arg1	microspheres					1122:1133	blank microspheres	1116:1133	blank microspheres	1116:1133	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	4	56	theme	stimuli-responsive	600:617	arg1	capacities					638:647	variable stimuli-responsive controlled release capacities	591:647	variable stimuli-responsive controlled release capacities	591:647	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	2	57	theme	pyrrolidone	302:312	arg1	microspheres					348:359	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres	257:359	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres	257:359	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	5	58	from	content	1010:1016	arg1	leaves					1021:1026	leaves	1021:1026	leaves	1021:1026	In addition, blank microspheres improved the growth and physiological activity of cucumber seedlings, reduced copper content in leaves, increased soil nutrient content, and prevented soil acidification.					
36521750	7	59	theme	functional	1391:1400	arg1	bacteria					1402:1409	these beneficial functional bacteria	1374:1409	these beneficial functional bacteria	1374:1409	Redundancy analysis (RDA) and Spearman correlation analysis showed that these beneficial functional bacteria were mainly positively correlated with soil EC, A-N, and N-N.					
36521750	4	60	theme	release	630:636	arg1	capacities					638:647	variable stimuli-responsive controlled release capacities	591:647	variable stimuli-responsive controlled release capacities	591:647	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	6	61	theme	bacteria	1198:1205	arg1	communities					1207:1217	soil beneficial functional bacteria communities	1171:1217	soil beneficial functional bacteria communities	1171:1217	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	5	62	theme	nutrient	1044:1051	arg1	content					1053:1059	soil nutrient content	1039:1059	soil nutrient content	1039:1059	In addition, blank microspheres improved the growth and physiological activity of cucumber seedlings, reduced copper content in leaves, increased soil nutrient content, and prevented soil acidification.					
36521750	4	63	from	application	786:796	arg1	stage					811:815	the early stage	801:815	the early stage	801:815	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	1	64	theme	Excessive	148:156	arg1	copper					158:163	Excessive copper	148:163	Excessive copper (Cu) concentrations	148:183	Excessive copper (Cu) concentrations pose significant health risks to both plants and humans.					
36521750	1	64	theme	Excessive	148:156	arg1	Cu					166:167	Cu	166:167	Cu	166:167	Excessive copper (Cu) concentrations pose significant health risks to both plants and humans.					
36521750	7	65	theme	correlation	1341:1351	arg1	analysis					1353:1360	Spearman correlation analysis	1332:1360	Spearman correlation analysis	1332:1360	Redundancy analysis (RDA) and Spearman correlation analysis showed that these beneficial functional bacteria were mainly positively correlated with soil EC, A-N, and N-N.					
36521750	5	66	dep	growth	938:943	arg1	the					934:936	the	934:936	the	934:936	In addition, blank microspheres improved the growth and physiological activity of cucumber seedlings, reduced copper content in leaves, increased soil nutrient content, and prevented soil acidification.					
36521750	4	67	theme	soil	688:691	arg1	types					679:683	types	679:683	types of soil	679:691	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	4	67	theme	soil	688:691	arg1	temperatures					662:673	different temperatures	652:673	different temperatures	652:673	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	0	68	theme	effects	107:113	arg1	reduction					86:94	reduction	86:94	reduction of adverse effects of copper in agricultural soils	86:145	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	0	68	theme	effects	107:113	arg1	pesticides					71:80	pesticides	71:80	pesticides	71:80	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	3	69	dep	content	418:424	arg1	The					406:408	The	406:408	The	406:408	The loading content and encapsulation efficiency of optimal microspheres determined by physical modifications were 19.77% and 99.32%, respectively.					
36521750	4	70	theme	larvae	741:746	arg1	efficiency					727:736	better control efficiency	712:736	better control efficiency of larvae of Protaetia brevitarsis	712:771	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	0	71	theme	copper	118:123	arg1	effects					107:113	adverse effects	99:113	adverse effects of copper	99:123	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	4	72	theme	Protaetia	751:759	arg1	brevitarsis					761:771	Protaetia brevitarsis	751:771	Protaetia brevitarsis	751:771	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	4	73	theme	better	712:717	arg1	efficiency					727:736	better control efficiency	712:736	better control efficiency of larvae of Protaetia brevitarsis	712:771	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
36521750	2	74	theme	dinotefuran	330:340	arg1	microspheres					348:359	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres	257:359	sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres	257:359	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	6	75	theme	heavy	1234:1238	arg1	immobilization/tolerance					1251:1274	heavy metal (HM) immobilization/tolerance	1234:1274	heavy metal (HM) immobilization/tolerance	1234:1274	Further, the use of blank microspheres increased the relative abundance of soil beneficial functional bacteria communities, which mediate heavy metal (HM) immobilization/tolerance and promote plant growth.					
36521750	7	76	theme	Redundancy	1302:1311	arg1	RDA					1323:1325	RDA	1323:1325	RDA	1323:1325	Redundancy analysis (RDA) and Spearman correlation analysis showed that these beneficial functional bacteria were mainly positively correlated with soil EC, A-N, and N-N.					
36521750	7	76	theme	Redundancy	1302:1311	arg1	analysis					1313:1320	Redundancy analysis	1302:1320	Redundancy analysis (RDA)	1302:1326	Redundancy analysis (RDA) and Spearman correlation analysis showed that these beneficial functional bacteria were mainly positively correlated with soil EC, A-N, and N-N.					
36521750	2	77	theme	spray-drying	381:392	arg1	technology					394:403	spray-drying technology	381:403	spray-drying technology	381:403	In this study, sodium alginate (SA)-gelatin (GEL)-polyvinyl pyrrolidone (PVP)- embedded dinotefuran (DIN) microspheres were prepared using spray-drying technology.					
36521750	8	78	theme	carrier	1555:1561	arg1	materials					1563:1571	physically modified carrier materials	1535:1571	physically modified carrier materials	1535:1571	In summary, this study showed that the technique of combining physically modified carrier materials with pesticides has the potential to reduce Cu contamination in the surrounding agricultural soil during pesticide application, thereby reducing Cu uptake by crops.					
36521750	3	79	theme	encapsulation	430:442	arg1	efficiency					444:453	encapsulation efficiency	430:453	encapsulation efficiency	430:453	The loading content and encapsulation efficiency of optimal microspheres determined by physical modifications were 19.77% and 99.32%, respectively.					
36521750	0	80	theme	controlled	49:58	arg1	release					60:66	controlled release	49:66	controlled release of pesticides and reduction of adverse effects of copper in agricultural soils	49:145	Sodium alginate-based composite microspheres for controlled release of pesticides and reduction of adverse effects of copper in agricultural soils.					
36521750	5	81	theme	copper	1003:1008	arg1	content					1010:1016	copper content	1003:1016	copper content in leaves	1003:1026	In addition, blank microspheres improved the growth and physiological activity of cucumber seedlings, reduced copper content in leaves, increased soil nutrient content, and prevented soil acidification.					
36521750	3	82	theme	microspheres	466:477	arg1	content					418:424	loading content	410:424	loading content	410:424	The loading content and encapsulation efficiency of optimal microspheres determined by physical modifications were 19.77% and 99.32%, respectively.					
36521750	3	82	theme	microspheres	466:477	arg1	efficiency					444:453	encapsulation efficiency	430:453	encapsulation efficiency	430:453	The loading content and encapsulation efficiency of optimal microspheres determined by physical modifications were 19.77% and 99.32%, respectively.					
36521750	8	83	theme	agricultural	1653:1664	arg1	soil					1666:1669	the surrounding agricultural soil	1637:1669	the surrounding agricultural soil	1637:1669	In summary, this study showed that the technique of combining physically modified carrier materials with pesticides has the potential to reduce Cu contamination in the surrounding agricultural soil during pesticide application, thereby reducing Cu uptake by crops.					
36521750	5	84	theme	cucumber	975:982	arg1	seedlings					984:992	cucumber seedlings	975:992	cucumber seedlings	975:992	In addition, blank microspheres improved the growth and physiological activity of cucumber seedlings, reduced copper content in leaves, increased soil nutrient content, and prevented soil acidification.					
36521750	4	85	theme	variable	591:598	arg1	capacities					638:647	variable stimuli-responsive controlled release capacities	591:647	variable stimuli-responsive controlled release capacities	591:647	In addition, the microspheres showed variable stimuli-responsive controlled release capacities in different temperatures and types of soil, as well as showed better control efficiency of larvae of Protaetia brevitarsis at pesticide application in the early stage, with the potential ability to control pest outbreaks at high temperatures.					
35763923	0	0	theme	antioxidant	107:117	arg1	activity					119:126	antioxidant activity	107:126	antioxidant activity	107:126	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.					
35763923	2	1	from	property	471:478	arg1	treatment					507:515	the pH-shifting treatment	491:515	the pH-shifting treatment	491:515	Intrinsic fluorescence and far-UV CD spectra revealed that glycation did not affect the self-assembly property of β-CG in the pH-shifting treatment.					
35763923	2	2	theme	far-UV	396:401	arg1	spectra					406:412	far-UV CD spectra	396:412	far-UV CD spectra	396:412	Intrinsic fluorescence and far-UV CD spectra revealed that glycation did not affect the self-assembly property of β-CG in the pH-shifting treatment.					
35763923	7	3	contain	has	1122:1124	arg2	potential					1134:1142	immense potential	1126:1142	immense potential to become a new delivery carrier for hydrophobic food components by means of a self-assembly strategy	1126:1244	Thus, β-CG-DEX has immense potential to become a new delivery carrier for hydrophobic food components by means of a self-assembly strategy.					
35763923	7	3	contain	has	1122:1124	arg1	β-CG-DEX					1113:1120	β-CG-DEX	1113:1120	β-CG-DEX	1113:1120	Thus, β-CG-DEX has immense potential to become a new delivery carrier for hydrophobic food components by means of a self-assembly strategy.					
35763923	7	4	theme	self-assembly	1223:1235	arg1	strategy					1237:1244	a self-assembly strategy	1221:1244	a self-assembly strategy	1221:1244	Thus, β-CG-DEX has immense potential to become a new delivery carrier for hydrophobic food components by means of a self-assembly strategy.					
35763923	1	5	theme	glycated	187:194	arg1	β-CG					215:218	β-CG	215:218	β-CG	215:218	In this study, glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites were fabricated by a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX).					
35763923	1	5	theme	glycated	187:194	arg1	β-conglycinin					200:212	glycated soy β-conglycinin	187:212	glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites	187:256	In this study, glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites were fabricated by a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX).					
35763923	6	6	theme	gastrointestinal	969:984	arg1	condition					986:994	the simulated gastrointestinal condition	955:994	the simulated gastrointestinal condition	955:994	During the simulated gastrointestinal condition, compared with β-CG-Cur, β-CG-DEX-3d-Cur nanoparticles showed a more stable and sustained release of curcumin.					
35763923	7	7	theme	immense	1126:1132	arg1	potential					1134:1142	immense potential	1126:1142	immense potential to become a new delivery carrier for hydrophobic food components by means of a self-assembly strategy	1126:1244	Thus, β-CG-DEX has immense potential to become a new delivery carrier for hydrophobic food components by means of a self-assembly strategy.					
35763923	1	8	theme	unique	279:284	arg1	character					311:319	a unique reversible self-assembly character	277:319	a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX)	277:366	In this study, glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites were fabricated by a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX).					
35763923	5	9	theme	higher	913:918	arg1	times					907:911	approximately 1.5 times	889:911	approximately 1.5 times higher than that of free curcumin	889:945	Moreover, the reassembled β-CG-DEX-3d-Cur showed eminent antioxidant activity of approximately 1.5 times higher than that of free curcumin.					
35763923	6	10	theme	curcumin	1097:1104	arg1	release					1086:1092	a more stable and sustained release	1058:1092	a more stable and sustained release of curcumin	1058:1104	During the simulated gastrointestinal condition, compared with β-CG-Cur, β-CG-DEX-3d-Cur nanoparticles showed a more stable and sustained release of curcumin.					
35763923	1	11	theme	soy	196:198	arg1	β-CG					215:218	β-CG	215:218	β-CG	215:218	In this study, glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites were fabricated by a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX).					
35763923	1	11	theme	soy	196:198	arg1	β-conglycinin					200:212	glycated soy β-conglycinin	187:212	glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites	187:256	In this study, glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites were fabricated by a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX).					
35763923	0	12	theme	sustained-release	132:148	arg1	property					150:157	sustained-release property	132:157	sustained-release property	132:157	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.					
35763923	7	13	theme	new	1156:1158	arg1	carrier					1169:1175	a new delivery carrier	1154:1175	a new delivery carrier for hydrophobic food components	1154:1207	Thus, β-CG-DEX has immense potential to become a new delivery carrier for hydrophobic food components by means of a self-assembly strategy.					
35763923	0	14	dep	activity	119:126	arg1	the					103:105	the	103:105	the	103:105	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.					
35763923	5	15	theme	eminent	857:863	arg1	activity					877:884	eminent antioxidant activity	857:884	eminent antioxidant activity of approximately 1.5 times higher than that of free curcumin	857:945	Moreover, the reassembled β-CG-DEX-3d-Cur showed eminent antioxidant activity of approximately 1.5 times higher than that of free curcumin.					
35763923	7	16	theme	hydrophobic	1181:1191	arg1	components					1198:1207	hydrophobic food components	1181:1207	hydrophobic food components	1181:1207	Thus, β-CG-DEX has immense potential to become a new delivery carrier for hydrophobic food components by means of a self-assembly strategy.					
35763923	5	17	theme	free	933:936	arg1	curcumin					938:945	free curcumin	933:945	free curcumin	933:945	Moreover, the reassembled β-CG-DEX-3d-Cur showed eminent antioxidant activity of approximately 1.5 times higher than that of free curcumin.					
35763923	5	18	theme	antioxidant	865:875	arg1	activity					877:884	eminent antioxidant activity	857:884	eminent antioxidant activity of approximately 1.5 times higher than that of free curcumin	857:945	Moreover, the reassembled β-CG-DEX-3d-Cur showed eminent antioxidant activity of approximately 1.5 times higher than that of free curcumin.					
35763923	2	19	theme	β-CG	483:486	arg1	property					471:478	the self-assembly property	453:478	the self-assembly property of β-CG in the pH-shifting treatment	453:515	Intrinsic fluorescence and far-UV CD spectra revealed that glycation did not affect the self-assembly property of β-CG in the pH-shifting treatment.					
35763923	1	20	theme	reversible	286:295	arg1	character					311:319	a unique reversible self-assembly character	277:319	a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX)	277:366	In this study, glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites were fabricated by a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX).					
35763923	6	21	theme	sustained	1076:1084	arg1	release					1086:1092	a more stable and sustained release	1058:1092	a more stable and sustained release of curcumin	1058:1104	During the simulated gastrointestinal condition, compared with β-CG-Cur, β-CG-DEX-3d-Cur nanoparticles showed a more stable and sustained release of curcumin.					
35763923	1	22	theme	self-assembly	297:309	arg1	character					311:319	a unique reversible self-assembly character	277:319	a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX)	277:366	In this study, glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites were fabricated by a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX).					
35763923	0	23	theme	curcumin	162:169	arg1	activity					119:126	antioxidant activity	107:126	antioxidant activity	107:126	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.					
35763923	0	23	theme	curcumin	162:169	arg1	property					150:157	sustained-release property	132:157	sustained-release property	132:157	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.					
35763923	3	24	dep	pH	616:617	arg1	to					624:625	to	624:625	to	624:625	The structure of β-CG-DEX could be unfolded at pH 12.0 and reassembled during acidification (from pH 12.0 to 7.0).					
35763923	3	25	dep	unfolded	553:560	arg1	pH					616:617	pH 12.0 to 7.0	616:629	pH 12.0 to 7.0	616:629	The structure of β-CG-DEX could be unfolded at pH 12.0 and reassembled during acidification (from pH 12.0 to 7.0).					
35763923	2	26	theme	self-assembly	457:469	arg1	property					471:478	the self-assembly property	453:478	the self-assembly property of β-CG in the pH-shifting treatment	453:515	Intrinsic fluorescence and far-UV CD spectra revealed that glycation did not affect the self-assembly property of β-CG in the pH-shifting treatment.					
35763923	6	27	theme	simulated	959:967	arg1	condition					986:994	the simulated gastrointestinal condition	955:994	the simulated gastrointestinal condition	955:994	During the simulated gastrointestinal condition, compared with β-CG-Cur, β-CG-DEX-3d-Cur nanoparticles showed a more stable and sustained release of curcumin.					
35763923	5	28	theme	times	907:911	arg1	activity					877:884	eminent antioxidant activity	857:884	eminent antioxidant activity of approximately 1.5 times higher than that of free curcumin	857:945	Moreover, the reassembled β-CG-DEX-3d-Cur showed eminent antioxidant activity of approximately 1.5 times higher than that of free curcumin.					
35763923	6	29	theme	stable	1065:1070	arg1	release					1086:1092	a more stable and sustained release	1058:1092	a more stable and sustained release of curcumin	1058:1104	During the simulated gastrointestinal condition, compared with β-CG-Cur, β-CG-DEX-3d-Cur nanoparticles showed a more stable and sustained release of curcumin.					
35763923	0	30	theme	β-conglycinin-dextran-polyphenol	40:71	arg1	nanocomplexes					73:85	soy β-conglycinin-dextran-polyphenol nanocomplexes	36:85	soy β-conglycinin-dextran-polyphenol nanocomplexes	36:85	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.					
35763923	3	31	theme	β-CG-DEX	535:542	arg1	structure					522:530	The structure	518:530	The structure of β-CG-DEX	518:542	The structure of β-CG-DEX could be unfolded at pH 12.0 and reassembled during acidification (from pH 12.0 to 7.0).					
35763923	5	32	theme	reassembled	822:832	arg1	β-CG-DEX-3d-Cur					834:848	the reassembled β-CG-DEX-3d-Cur	818:848	the reassembled β-CG-DEX-3d-Cur	818:848	Moreover, the reassembled β-CG-DEX-3d-Cur showed eminent antioxidant activity of approximately 1.5 times higher than that of free curcumin.					
35763923	1	33	theme	curcumin	232:239	arg1	composites					247:256	curcumin (Cur) composites	232:256	curcumin (Cur) composites	232:256	In this study, glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites were fabricated by a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX).					
35763923	0	34	theme	soy	36:38	arg1	nanocomplexes					73:85	soy β-conglycinin-dextran-polyphenol nanocomplexes	36:85	soy β-conglycinin-dextran-polyphenol nanocomplexes	36:85	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.					
35763923	1	35	theme	β-conglycinin-dextran	324:344	arg1	conjugates					346:355	β-conglycinin-dextran conjugates	324:355	β-conglycinin-dextran conjugates (β-CG-DEX)	324:366	In this study, glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites were fabricated by a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX).					
35763923	1	35	theme	β-conglycinin-dextran	324:344	arg1	β-CG-DEX					358:365	β-CG-DEX	358:365	β-CG-DEX	358:365	In this study, glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites were fabricated by a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX).					
35763923	7	36	theme	delivery	1160:1167	arg1	carrier					1169:1175	a new delivery carrier	1154:1175	a new delivery carrier for hydrophobic food components	1154:1207	Thus, β-CG-DEX has immense potential to become a new delivery carrier for hydrophobic food components by means of a self-assembly strategy.					
35763923	2	37	theme	pH-shifting	495:505	arg1	treatment					507:515	the pH-shifting treatment	491:515	the pH-shifting treatment	491:515	Intrinsic fluorescence and far-UV CD spectra revealed that glycation did not affect the self-assembly property of β-CG in the pH-shifting treatment.					
35763923	1	38	theme	conjugates	346:355	arg1	character					311:319	a unique reversible self-assembly character	277:319	a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX)	277:366	In this study, glycated soy β-conglycinin (β-CG) stabilized curcumin (Cur) composites were fabricated by a unique reversible self-assembly character of β-conglycinin-dextran conjugates (β-CG-DEX).					
35763923	0	39	from	Improvement	88:98	arg1	activity					119:126	antioxidant activity	107:126	antioxidant activity	107:126	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.					
35763923	0	39	from	Improvement	88:98	arg1	property					150:157	sustained-release property	132:157	sustained-release property	132:157	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.					
35763923	4	40	theme	loading	715:721	arg1	123.4 mg/g					733:742	123.4 mg/g	733:742	123.4 mg/g	733:742	Meanwhile, β-CG-DEX-3d, which was incubated at 60 °C for 3 days, exhibited a high loading capacity (123.4 mg/g) for curcumin, which far exceeds that (74.90 mg/g) of β-CG-Cur.					
35763923	4	40	theme	loading	715:721	arg1	capacity					723:730	a high loading capacity	708:730	a high loading capacity (123.4 mg/g)	708:743	Meanwhile, β-CG-DEX-3d, which was incubated at 60 °C for 3 days, exhibited a high loading capacity (123.4 mg/g) for curcumin, which far exceeds that (74.90 mg/g) of β-CG-Cur.					
35763923	0	41	theme	nanocomplexes	73:85	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.					
35763923	0	41	theme	nanocomplexes	73:85	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.					
35763923	2	42	theme	Intrinsic	369:377	arg1	fluorescence					379:390	Intrinsic fluorescence	369:390	Intrinsic fluorescence	369:390	Intrinsic fluorescence and far-UV CD spectra revealed that glycation did not affect the self-assembly property of β-CG in the pH-shifting treatment.					
35763923	6	43	theme	β-CG-DEX-3d-Cur	1021:1035	arg1	nanoparticles					1037:1049	β-CG-DEX-3d-Cur nanoparticles	1021:1049	β-CG-DEX-3d-Cur nanoparticles	1021:1049	During the simulated gastrointestinal condition, compared with β-CG-Cur, β-CG-DEX-3d-Cur nanoparticles showed a more stable and sustained release of curcumin.					
35763923	0	44	dep	Fabrication	0:10	arg1	Improvement					88:98	Improvement	88:98	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.	0:170	Fabrication and characterization of soy β-conglycinin-dextran-polyphenol nanocomplexes: Improvement on the antioxidant activity and sustained-release property of curcumin.					
35763923	2	45	theme	CD	403:404	arg1	spectra					406:412	far-UV CD spectra	396:412	far-UV CD spectra	396:412	Intrinsic fluorescence and far-UV CD spectra revealed that glycation did not affect the self-assembly property of β-CG in the pH-shifting treatment.					
35763923	7	46	theme	food	1193:1196	arg1	components					1198:1207	hydrophobic food components	1181:1207	hydrophobic food components	1181:1207	Thus, β-CG-DEX has immense potential to become a new delivery carrier for hydrophobic food components by means of a self-assembly strategy.					
35763923	4	47	theme	high	710:713	arg1	123.4 mg/g					733:742	123.4 mg/g	733:742	123.4 mg/g	733:742	Meanwhile, β-CG-DEX-3d, which was incubated at 60 °C for 3 days, exhibited a high loading capacity (123.4 mg/g) for curcumin, which far exceeds that (74.90 mg/g) of β-CG-Cur.					
35763923	4	47	theme	high	710:713	arg1	capacity					723:730	a high loading capacity	708:730	a high loading capacity (123.4 mg/g)	708:743	Meanwhile, β-CG-DEX-3d, which was incubated at 60 °C for 3 days, exhibited a high loading capacity (123.4 mg/g) for curcumin, which far exceeds that (74.90 mg/g) of β-CG-Cur.					
36531989	7	0	theme	B.	1164:1165	arg1	Bv46					1176:1179	B. vulgatus Bv46	1164:1179	B. vulgatus Bv46	1164:1179	B. vulgatus Bv46 modified the gut microbiota community of colitis mice and observably increased the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level.					
36531989	8	1	theme	IL-6	1532:1535	arg1	secretion					1502:1510	the secretion	1498:1510	the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro	1498:1577	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	8	2	theme	B	1717:1717	arg1	responses					1724:1732	B cell responses	1717:1732	B cell responses	1717:1732	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	6	3	theme	colon	1099:1103	arg1	shortening					1105:1114	colon shortening	1099:1114	colon shortening	1099:1114	Results and Discussion B. vulgatus Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage.					
36531989	8	4	theme	responses	1724:1732	arg1	regulation					1703:1712	the regulation	1699:1712	the regulation of B cell responses	1699:1732	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	3	5	theme	B.	513:514	arg1	Bv46					525:528	B. vulgatus Bv46	513:528	B. vulgatus Bv46	513:528	Methods Female C57BL/6J mice were given 3% DSS in drinking water to induce colitis and simultaneously treated with B. vulgatus Bv46 by gavage for 7 days.					
36531989	9	6	theme	B.	1771:1772	arg1	Bv46					1783:1786	B. vulgatus Bv46	1771:1786	B. vulgatus Bv46	1771:1786	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	3	7	from	DSS	441:443	arg1	water					457:461	drinking water	448:461	drinking water	448:461	Methods Female C57BL/6J mice were given 3% DSS in drinking water to induce colitis and simultaneously treated with B. vulgatus Bv46 by gavage for 7 days.					
36531989	2	8	theme	study	270:274	arg1	aim					258:260	The aim	254:260	The aim of this study	254:274	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	8	9	theme	Ccl19	1616:1620	arg1	genes					1650:1654	Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes	1616:1654	Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes	1616:1654	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	7	10	theme	genus	1341:1345	arg1	level					1347:1351	the genus level	1337:1351	the genus level	1337:1351	B. vulgatus Bv46 modified the gut microbiota community of colitis mice and observably increased the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level.					
36531989	9	11	theme	Bv46	1783:1786	arg1	administration					1753:1766	oral administration	1748:1766	oral administration of B. vulgatus Bv46	1748:1786	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	8	12	theme	Cd19	1623:1626	arg1	genes					1650:1654	Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes	1616:1654	Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes	1616:1654	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	7	13	theme	Anaerotignum	1307:1318	arg1	abundance					1264:1272	the abundance	1260:1272	the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level	1260:1351	B. vulgatus Bv46 modified the gut microbiota community of colitis mice and observably increased the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level.					
36531989	8	14	theme	Cd22	1629:1632	arg1	genes					1650:1654	Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes	1616:1654	Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes	1616:1654	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	5	15	theme	chain	758:762	arg1	SCFAs					777:781	SCFAs	777:781	SCFAs	777:781	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	15	theme	chain	758:762	arg1	acids					770:774	fecal short chain fatty acids	746:774	fecal short chain fatty acids (SCFAs) concentration	746:796	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	8	16	theme	B.	1367:1368	arg1	treatment					1384:1392	B. vulgatus Bv46 treatment	1367:1392	B. vulgatus Bv46 treatment	1367:1392	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	8	17	theme	Cd40	1635:1638	arg1	genes					1650:1654	Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes	1616:1654	Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes	1616:1654	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	5	18	theme	acids	770:774	arg1	concentration					784:796	fecal short chain fatty acids (SCFAs) concentration	746:796	fecal short chain fatty acids (SCFAs) concentration	746:796	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	8	19	theme	Bv46	1379:1382	arg1	treatment					1384:1392	B. vulgatus Bv46 treatment	1367:1392	B. vulgatus Bv46 treatment	1367:1392	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	6	20	theme	histopathological	1138:1154	arg1	damage					1156:1161	colon histopathological damage	1132:1161	colon histopathological damage	1132:1161	Results and Discussion B. vulgatus Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage.					
36531989	9	21	theme	fecal	1822:1826	arg1	SCFAs					1828:1832	fecal SCFAs	1822:1832	fecal SCFAs	1822:1832	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	8	22	theme	Cxcr5	1644:1648	arg1	genes					1650:1654	Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes	1616:1654	Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes	1616:1654	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	5	23	theme	colonic	823:829	arg1	level					840:844	colonic cytokine level	823:844	colonic cytokine level	823:844	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	4	24	theme	histological	646:657	arg1	changes					659:665	the colon length and histological changes	625:665	changes	659:665	Daily weight and disease activity index (DAI) of mice were recorded, and the colon length and histological changes were evaluated.					
36531989	3	25	dep	B.	513:514	arg1	vulgatus					516:523	vulgatus	516:523	vulgatus	516:523	Methods Female C57BL/6J mice were given 3% DSS in drinking water to induce colitis and simultaneously treated with B. vulgatus Bv46 by gavage for 7 days.					
36531989	9	26	theme	butyric	1846:1852	arg1	acid					1854:1857	butyric acid	1846:1857	butyric acid	1846:1857	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	9	26	theme	butyric	1846:1852	arg1	contents					1810:1817	the contents	1806:1817	the contents of fecal SCFAs	1806:1832	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	0	27	theme	gut	77:79	arg1	microbiota					81:90	gut microbiota	77:90	gut microbiota	77:90	Bacteroides vulgatus attenuates experimental mice colitis through modulating gut microbiota and immune responses.					
36531989	1	28	theme	predominant	162:172	arg1	species					186:192	the predominant Bacteroides species	158:192	the predominant Bacteroides species in the human gut	158:209	Introduction Bacteroides vulgatus is one of the predominant Bacteroides species in the human gut and exerts a series of beneficial effects.					
36531989	8	29	theme	colonic	1422:1428	arg1	TNF-α					1430:1434	colonic TNF-α	1422:1434	colonic TNF-α	1422:1434	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	5	30	theme	B.	698:699	arg1	Bv46					710:713	B. vulgatus Bv46	698:713	B. vulgatus Bv46	698:713	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	1	31	theme	species	186:192	arg1	one					151:153	one	151:153	one	151:153	Introduction Bacteroides vulgatus is one of the predominant Bacteroides species in the human gut and exerts a series of beneficial effects.					
36531989	1	31	theme	species	186:192	arg1	species					186:192	the predominant Bacteroides species	158:192	the predominant Bacteroides species in the human gut	158:209	Introduction Bacteroides vulgatus is one of the predominant Bacteroides species in the human gut and exerts a series of beneficial effects.					
36531989	8	32	from	expression	1408:1417	arg1	colitis					1473:1479	DSS-induced mouse colitis	1455:1479	DSS-induced mouse colitis in vivo	1455:1487	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	5	33	theme	264·7	876:880	arg1	line					898:901	RAW 264·7 macrophage cell line	872:901	RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS)	872:943	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	2	34	theme	induced	369:375	arg1	model					391:395	a dextran sodium sulfate (DSS) induced colitis mouse model	338:395	a dextran sodium sulfate (DSS) induced colitis mouse model	338:395	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	5	35	theme	cell	893:896	arg1	line					898:901	RAW 264·7 macrophage cell line	872:901	RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS)	872:943	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	6	36	theme	B.	983:984	arg1	Bv46					995:998	Discussion B. vulgatus Bv46	972:998	Discussion B. vulgatus Bv46	972:998	Results and Discussion B. vulgatus Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage.					
36531989	3	37	theme	Methods	398:404	arg1	mice					422:425	Methods Female C57BL/6J mice	398:425	Methods Female C57BL/6J mice	398:425	Methods Female C57BL/6J mice were given 3% DSS in drinking water to induce colitis and simultaneously treated with B. vulgatus Bv46 by gavage for 7 days.					
36531989	0	38	theme	Bacteroides	0:10	arg1	vulgatus					12:19	Bacteroides vulgatus	0:19	Bacteroides vulgatus	0:19	Bacteroides vulgatus attenuates experimental mice colitis through modulating gut microbiota and immune responses.					
36531989	5	39	theme	cytokine	850:857	arg1	secretion					859:867	cytokine secretion	850:867	cytokine secretion	850:867	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	8	40	theme	mouse	1467:1471	arg1	colitis					1473:1479	DSS-induced mouse colitis	1455:1479	DSS-induced mouse colitis in vivo	1455:1487	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	5	41	theme	fecal	746:750	arg1	SCFAs					777:781	SCFAs	777:781	SCFAs	777:781	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	41	theme	fecal	746:750	arg1	acids					770:774	fecal short chain fatty acids	746:774	fecal short chain fatty acids (SCFAs) concentration	746:796	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	1	42	theme	Bacteroides	127:137	arg1	Introduction					114:125	Introduction	114:125	Introduction Bacteroides vulgatus	114:146	Introduction Bacteroides vulgatus is one of the predominant Bacteroides species in the human gut and exerts a series of beneficial effects.					
36531989	10	43	theme	vulgatus	1977:1984	arg1	Bv46					1986:1989	B. vulgatus Bv46	1974:1989	B. vulgatus Bv46	1974:1989	Supplementation with B. vulgatus Bv46 serves as a promising strategy for the prevention of colitis.					
36531989	2	44	theme	mouse	385:389	arg1	model					391:395	a dextran sodium sulfate (DSS) induced colitis mouse model	338:395	a dextran sodium sulfate (DSS) induced colitis mouse model	338:395	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	0	45	theme	mice	45:48	arg1	colitis					50:56	experimental mice colitis	32:56	experimental mice colitis	32:56	Bacteroides vulgatus attenuates experimental mice colitis through modulating gut microbiota and immune responses.					
36531989	5	46	theme	Bv46	710:713	arg1	effects					687:693	The effects	683:693	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS)	683:943	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	7	47	theme	colitis	1222:1228	arg1	mice					1230:1233	colitis mice	1222:1233	colitis mice	1222:1233	B. vulgatus Bv46 modified the gut microbiota community of colitis mice and observably increased the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level.					
36531989	9	48	theme	B.	1935:1936	arg1	Bv46					1947:1950	B. vulgatus Bv46	1935:1950	B. vulgatus Bv46	1935:1950	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	8	49	from	expression	1602:1611	arg1	colon					1664:1668	mice colon	1659:1668	mice colon	1659:1668	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	3	50	theme	drinking	448:455	arg1	water					457:461	drinking water	448:461	drinking water	448:461	Methods Female C57BL/6J mice were given 3% DSS in drinking water to induce colitis and simultaneously treated with B. vulgatus Bv46 by gavage for 7 days.					
36531989	5	51	theme	gut	718:720	arg1	composition					733:743	gut microbiota composition	718:743	gut microbiota composition	718:743	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	6	52	theme	colitis	1049:1055	arg1	symptoms					1025:1032	symptoms	1025:1032	symptoms of DSS-induced colitis	1025:1055	Results and Discussion B. vulgatus Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage.					
36531989	9	53	theme	Bv46	1947:1950	arg1	effect					1925:1930	the anti-inflammatory effect	1903:1930	the anti-inflammatory effect of B. vulgatus Bv46	1903:1950	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	2	54	theme	vulgatus	321:328	arg1	Bv46					330:333	B. vulgatus Bv46	318:333	B. vulgatus Bv46	318:333	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	7	55	theme	gut	1194:1196	arg1	community					1209:1217	the gut microbiota community	1190:1217	the gut microbiota community of colitis mice	1190:1233	B. vulgatus Bv46 modified the gut microbiota community of colitis mice and observably increased the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level.					
36531989	4	56	theme	Daily	552:556	arg1	weight					558:563	Daily weight	552:563	Daily weight	552:563	Daily weight and disease activity index (DAI) of mice were recorded, and the colon length and histological changes were evaluated.					
36531989	4	56	theme	Daily	552:556	arg1	DAI					593:595	DAI	593:595	DAI	593:595	Daily weight and disease activity index (DAI) of mice were recorded, and the colon length and histological changes were evaluated.					
36531989	2	57	dep	induced	369:375	arg1	DSS					364:366	DSS	364:366	DSS	364:366	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	2	57	dep	induced	369:375	arg1	sulfate					355:361	dextran sodium sulfate	340:361	dextran sodium sulfate (DSS)	340:367	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	1	58	theme	effects	245:251	arg1	series					224:229	a series	222:229	a series of beneficial effects	222:251	Introduction Bacteroides vulgatus is one of the predominant Bacteroides species in the human gut and exerts a series of beneficial effects.					
36531989	10	59	theme	B.	1974:1975	arg1	Bv46					1986:1989	B. vulgatus Bv46	1974:1989	B. vulgatus Bv46	1974:1989	Supplementation with B. vulgatus Bv46 serves as a promising strategy for the prevention of colitis.					
36531989	8	60	theme	cell	1719:1722	arg1	responses					1724:1732	B cell responses	1717:1732	B cell responses	1717:1732	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	2	61	theme	dextran	340:346	arg1	DSS					364:366	DSS	364:366	DSS	364:366	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	2	61	theme	dextran	340:346	arg1	sulfate					355:361	dextran sodium sulfate	340:361	dextran sodium sulfate (DSS)	340:367	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	9	62	theme	oral	1748:1751	arg1	administration					1753:1766	oral administration	1748:1766	oral administration of B. vulgatus Bv46	1748:1786	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	9	63	dep	increased	1796:1804	arg1	contribute					1889:1898	contribute	1889:1898	may contribute to the anti-inflammatory effect of B. vulgatus Bv46	1885:1950	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	8	64	from	secretion	1502:1510	arg1	macrophages					1540:1550	macrophages	1540:1550	macrophages stimulated by LPS in vitro	1540:1577	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	9	65	theme	vulgatus	1774:1781	arg1	Bv46					1783:1786	B. vulgatus Bv46	1771:1786	B. vulgatus Bv46	1771:1786	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	6	66	dep	mice	1060:1063	arg1	including					1066:1074	including	1066:1074	including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage	1066:1161	Results and Discussion B. vulgatus Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage.					
36531989	1	67	theme	human	201:205	arg1	gut					207:209	the human gut	197:209	the human gut	197:209	Introduction Bacteroides vulgatus is one of the predominant Bacteroides species in the human gut and exerts a series of beneficial effects.					
36531989	10	68	theme	promising	2003:2011	arg1	strategy					2013:2020	a promising strategy	2001:2020	a promising strategy for the prevention of colitis	2001:2050	Supplementation with B. vulgatus Bv46 serves as a promising strategy for the prevention of colitis.					
36531989	10	68	theme	promising	2003:2011	arg1	Supplementation					1953:1967	Supplementation	1953:1967	Supplementation with B. vulgatus Bv46	1953:1989	Supplementation with B. vulgatus Bv46 serves as a promising strategy for the prevention of colitis.					
36531989	6	69	dep	including	1066:1074	arg1	alleviated					1121:1130	alleviated	1121:1130	alleviated colon histopathological damage	1121:1161	Results and Discussion B. vulgatus Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage.					
36531989	6	69	dep	including	1066:1074	arg1	reduced					1076:1082	reduced	1076:1082	reduced DAI	1076:1086	Results and Discussion B. vulgatus Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage.					
36531989	6	69	dep	including	1066:1074	arg1	prevented					1089:1097	prevented	1089:1097	prevented colon shortening	1089:1114	Results and Discussion B. vulgatus Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage.					
36531989	2	70	theme	protective	299:308	arg1	role					310:313	the protective role	295:313	the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model	295:395	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	5	71	theme	short	752:756	arg1	SCFAs					777:781	SCFAs	777:781	SCFAs	777:781	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	71	theme	short	752:756	arg1	acids					770:774	fecal short chain fatty acids	746:774	fecal short chain fatty acids (SCFAs) concentration	746:796	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	72	dep	B.	698:699	arg1	vulgatus					701:708	vulgatus	701:708	vulgatus	701:708	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	8	73	theme	vulgatus	1370:1377	arg1	treatment					1384:1392	B. vulgatus Bv46 treatment	1367:1392	B. vulgatus Bv46 treatment	1367:1392	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	5	74	theme	colon	816:820	arg1	secretion					859:867	cytokine secretion	850:867	cytokine secretion	850:867	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	74	theme	colon	816:820	arg1	composition					733:743	gut microbiota composition	718:743	gut microbiota composition	718:743	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	74	theme	colon	816:820	arg1	concentration					784:796	fecal short chain fatty acids (SCFAs) concentration	746:796	fecal short chain fatty acids (SCFAs) concentration	746:796	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	74	theme	colon	816:820	arg1	transcriptome					799:811	transcriptome	799:811	transcriptome of colon	799:820	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	74	theme	colon	816:820	arg1	level					840:844	colonic cytokine level	823:844	colonic cytokine level	823:844	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	4	75	theme	activity	577:584	arg1	index					586:590	disease activity index	569:590	disease activity index	569:590	Daily weight and disease activity index (DAI) of mice were recorded, and the colon length and histological changes were evaluated.					
36531989	4	76	theme	mice	601:604	arg1	weight					558:563	Daily weight	552:563	Daily weight	552:563	Daily weight and disease activity index (DAI) of mice were recorded, and the colon length and histological changes were evaluated.					
36531989	4	76	theme	mice	601:604	arg1	DAI					593:595	DAI	593:595	DAI	593:595	Daily weight and disease activity index (DAI) of mice were recorded, and the colon length and histological changes were evaluated.					
36531989	4	76	theme	mice	601:604	arg1	index					586:590	disease activity index	569:590	disease activity index	569:590	Daily weight and disease activity index (DAI) of mice were recorded, and the colon length and histological changes were evaluated.					
36531989	5	77	theme	fatty	764:768	arg1	SCFAs					777:781	SCFAs	777:781	SCFAs	777:781	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	77	theme	fatty	764:768	arg1	acids					770:774	fecal short chain fatty acids	746:774	fecal short chain fatty acids (SCFAs) concentration	746:796	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	7	78	theme	Alistipes	1324:1332	arg1	abundance					1264:1272	the abundance	1260:1272	the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level	1260:1351	B. vulgatus Bv46 modified the gut microbiota community of colitis mice and observably increased the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level.					
36531989	6	79	theme	colon	1132:1136	arg1	damage					1156:1161	colon histopathological damage	1132:1161	colon histopathological damage	1132:1161	Results and Discussion B. vulgatus Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage.					
36531989	9	80	theme	SCFAs	1828:1832	arg1	acid					1854:1857	butyric acid	1846:1857	butyric acid	1846:1857	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	9	80	theme	SCFAs	1828:1832	arg1	acid					1873:1876	propionic acid	1863:1876	propionic acid	1863:1876	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	9	80	theme	SCFAs	1828:1832	arg1	contents					1810:1817	the contents	1806:1817	the contents of fecal SCFAs	1806:1832	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	4	81	theme	colon	629:633	arg1	length					635:640	the colon length and histological changes	625:665	length	635:640	Daily weight and disease activity index (DAI) of mice were recorded, and the colon length and histological changes were evaluated.					
36531989	10	82	with	Supplementation	1953:1967	arg1	Bv46					1986:1989	B. vulgatus Bv46	1974:1989	B. vulgatus Bv46	1974:1989	Supplementation with B. vulgatus Bv46 serves as a promising strategy for the prevention of colitis.					
36531989	5	83	theme	cytokine	831:838	arg1	level					840:844	colonic cytokine level	823:844	colonic cytokine level	823:844	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	7	84	theme	Bacteroides	1294:1304	arg1	abundance					1264:1272	the abundance	1260:1272	the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level	1260:1351	B. vulgatus Bv46 modified the gut microbiota community of colitis mice and observably increased the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level.					
36531989	5	85	from	effects	687:693	arg1	secretion					859:867	cytokine secretion	850:867	cytokine secretion	850:867	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	85	from	effects	687:693	arg1	composition					733:743	gut microbiota composition	718:743	gut microbiota composition	718:743	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	85	from	effects	687:693	arg1	level					840:844	colonic cytokine level	823:844	colonic cytokine level	823:844	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	85	from	effects	687:693	arg1	transcriptome					799:811	transcriptome	799:811	transcriptome of colon	799:820	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	85	from	effects	687:693	arg1	concentration					784:796	fecal short chain fatty acids (SCFAs) concentration	746:796	fecal short chain fatty acids (SCFAs) concentration	746:796	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	8	86	theme	genes	1650:1654	arg1	expression					1602:1611	the expression	1598:1611	the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses	1598:1732	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	7	87	from	level	1347:1351	arg1	abundance					1264:1272	the abundance	1260:1272	the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level	1260:1351	B. vulgatus Bv46 modified the gut microbiota community of colitis mice and observably increased the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level.					
36531989	8	88	theme	TNF-α	1430:1434	arg1	expression					1408:1417	the expression	1404:1417	the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo	1404:1487	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	10	89	theme	colitis	2044:2050	arg1	prevention					2030:2039	the prevention	2026:2039	the prevention of colitis	2026:2050	Supplementation with B. vulgatus Bv46 serves as a promising strategy for the prevention of colitis.					
36531989	1	90	dep	Bacteroides	127:137	arg1	vulgatus					139:146	vulgatus	139:146	vulgatus	139:146	Introduction Bacteroides vulgatus is one of the predominant Bacteroides species in the human gut and exerts a series of beneficial effects.					
36531989	0	91	theme	immune	96:101	arg1	responses					103:111	immune responses	96:111	immune responses	96:111	Bacteroides vulgatus attenuates experimental mice colitis through modulating gut microbiota and immune responses.					
36531989	8	92	theme	mice	1659:1662	arg1	colon					1664:1668	mice colon	1659:1668	mice colon	1659:1668	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	1	93	theme	Bacteroides	174:184	arg1	species					186:192	the predominant Bacteroides species	158:192	the predominant Bacteroides species in the human gut	158:209	Introduction Bacteroides vulgatus is one of the predominant Bacteroides species in the human gut and exerts a series of beneficial effects.					
36531989	8	94	theme	IL-1β	1437:1441	arg1	expression					1408:1417	the expression	1404:1417	the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo	1404:1487	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	9	95	theme	propionic	1863:1871	arg1	acid					1873:1876	propionic acid	1863:1876	propionic acid	1863:1876	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	9	95	theme	propionic	1863:1871	arg1	contents					1810:1817	the contents	1806:1817	the contents of fecal SCFAs	1806:1832	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	1	96	from	species	186:192	arg1	gut					207:209	the human gut	197:209	the human gut	197:209	Introduction Bacteroides vulgatus is one of the predominant Bacteroides species in the human gut and exerts a series of beneficial effects.					
36531989	5	97	theme	RAW	872:874	arg1	line					898:901	RAW 264·7 macrophage cell line	872:901	RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS)	872:943	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	2	98	from	role	310:313	arg1	model					391:395	a dextran sodium sulfate (DSS) induced colitis mouse model	338:395	a dextran sodium sulfate (DSS) induced colitis mouse model	338:395	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	8	99	theme	IL-6	1447:1450	arg1	expression					1408:1417	the expression	1404:1417	the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo	1404:1487	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	5	100	theme	macrophage	882:891	arg1	line					898:901	RAW 264·7 macrophage cell line	872:901	RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS)	872:943	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	8	101	theme	DSS-induced	1455:1465	arg1	colitis					1473:1479	DSS-induced mouse colitis	1455:1479	DSS-induced mouse colitis in vivo	1455:1487	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	5	102	theme	microbiota	722:731	arg1	composition					733:743	gut microbiota composition	718:743	gut microbiota composition	718:743	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	7	103	dep	B.	1164:1165	arg1	vulgatus					1167:1174	vulgatus	1167:1174	vulgatus	1167:1174	B. vulgatus Bv46 modified the gut microbiota community of colitis mice and observably increased the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level.					
36531989	6	104	theme	Discussion	972:981	arg1	Bv46					995:998	Discussion B. vulgatus Bv46	972:998	Discussion B. vulgatus Bv46	972:998	Results and Discussion B. vulgatus Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage.					
36531989	3	105	theme	Female	406:411	arg1	mice					422:425	Methods Female C57BL/6J mice	398:425	Methods Female C57BL/6J mice	398:425	Methods Female C57BL/6J mice were given 3% DSS in drinking water to induce colitis and simultaneously treated with B. vulgatus Bv46 by gavage for 7 days.					
36531989	0	106	theme	experimental	32:43	arg1	colitis					50:56	experimental mice colitis	32:56	experimental mice colitis	32:56	Bacteroides vulgatus attenuates experimental mice colitis through modulating gut microbiota and immune responses.					
36531989	7	107	theme	mice	1230:1233	arg1	community					1209:1217	the gut microbiota community	1190:1217	the gut microbiota community of colitis mice	1190:1233	B. vulgatus Bv46 modified the gut microbiota community of colitis mice and observably increased the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level.					
36531989	9	108	theme	anti-inflammatory	1907:1923	arg1	effect					1925:1930	the anti-inflammatory effect	1903:1930	the anti-inflammatory effect of B. vulgatus Bv46	1903:1950	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	6	109	dep	B.	983:984	arg1	vulgatus					986:993	vulgatus	986:993	vulgatus	986:993	Results and Discussion B. vulgatus Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage.					
36531989	3	110	theme	%	439:439	arg1	DSS					441:443	3% DSS	438:443	3% DSS in drinking water	438:461	Methods Female C57BL/6J mice were given 3% DSS in drinking water to induce colitis and simultaneously treated with B. vulgatus Bv46 by gavage for 7 days.					
36531989	3	111	theme	C57BL/6J	413:420	arg1	mice					422:425	Methods Female C57BL/6J mice	398:425	Methods Female C57BL/6J mice	398:425	Methods Female C57BL/6J mice were given 3% DSS in drinking water to induce colitis and simultaneously treated with B. vulgatus Bv46 by gavage for 7 days.					
36531989	8	112	theme	IL-1β	1522:1526	arg1	secretion					1502:1510	the secretion	1498:1510	the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro	1498:1577	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	2	113	theme	colitis	377:383	arg1	model					391:395	a dextran sodium sulfate (DSS) induced colitis mouse model	338:395	a dextran sodium sulfate (DSS) induced colitis mouse model	338:395	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	7	114	theme	microbiota	1198:1207	arg1	community					1209:1217	the gut microbiota community	1190:1217	the gut microbiota community of colitis mice	1190:1233	B. vulgatus Bv46 modified the gut microbiota community of colitis mice and observably increased the abundance of Parabacteroides, Bacteroides, Anaerotignum and Alistipes at the genus level.					
36531989	9	115	theme	vulgatus	1938:1945	arg1	Bv46					1947:1950	B. vulgatus Bv46	1935:1950	B. vulgatus Bv46	1935:1950	Furthermore, oral administration of B. vulgatus Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of B. vulgatus Bv46.					
36531989	2	116	theme	Bv46	330:333	arg1	role					310:313	the protective role	295:313	the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model	295:395	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	5	117	theme	line	898:901	arg1	secretion					859:867	cytokine secretion	850:867	cytokine secretion	850:867	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	117	theme	line	898:901	arg1	composition					733:743	gut microbiota composition	718:743	gut microbiota composition	718:743	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	117	theme	line	898:901	arg1	concentration					784:796	fecal short chain fatty acids (SCFAs) concentration	746:796	fecal short chain fatty acids (SCFAs) concentration	746:796	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	117	theme	line	898:901	arg1	transcriptome					799:811	transcriptome	799:811	transcriptome of colon	799:820	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	5	117	theme	line	898:901	arg1	level					840:844	colonic cytokine level	823:844	colonic cytokine level	823:844	The effects of B. vulgatus Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264·7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed.					
36531989	6	118	theme	DSS-induced	1037:1047	arg1	colitis					1049:1055	DSS-induced colitis	1037:1055	DSS-induced colitis	1037:1055	Results and Discussion B. vulgatus Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage.					
36531989	8	119	theme	TNF-α	1515:1519	arg1	secretion					1502:1510	the secretion	1498:1510	the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro	1498:1577	In addition, B. vulgatus Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis in vivo, reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro, and downregulated the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon, which mainly participate in the regulation of B cell responses.					
36531989	2	120	theme	B.	318:319	arg1	Bv46					330:333	B. vulgatus Bv46	318:333	B. vulgatus Bv46	318:333	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	1	121	theme	beneficial	234:243	arg1	effects					245:251	beneficial effects	234:251	beneficial effects	234:251	Introduction Bacteroides vulgatus is one of the predominant Bacteroides species in the human gut and exerts a series of beneficial effects.					
36531989	4	122	theme	disease	569:575	arg1	index					586:590	disease activity index	569:590	disease activity index	569:590	Daily weight and disease activity index (DAI) of mice were recorded, and the colon length and histological changes were evaluated.					
36531989	2	123	theme	sodium	348:353	arg1	DSS					364:366	DSS	364:366	DSS	364:366	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36531989	2	123	theme	sodium	348:353	arg1	sulfate					355:361	dextran sodium sulfate	340:361	dextran sodium sulfate (DSS)	340:367	The aim of this study was to investigate the protective role of B. vulgatus Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model.					
36801218	1	0	from	alginate	297:304	arg1	presence					318:325	the presence	314:325	the presence of silver nanoparticles (Ag NPs)	314:358	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	2	1	from	effect	365:370	arg1	content					422:428	gel content	418:428	gel content	418:428	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	2	1	from	effect	365:370	arg1	characters					443:452	swelling characters	434:452	swelling characters	434:452	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	5	2	theme	gamma	828:832	arg1	irradiation					834:844	gamma irradiation	828:844	gamma irradiation	828:844	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	2	3	dep	content	422:428	arg1	the					414:416	the	414:416	the	414:416	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	0	4	theme	gamma-radiation	82:96	arg1	copolymerization					98:113	gamma-radiation copolymerization	82:113	gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	82:179	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	0	5	theme	AG	166:167	arg1	NPs					177:179	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	118:179	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	118:179	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	5	6	theme	irradiation	834:844	arg1	swelling					915:922	the highest swelling	903:922	the highest swelling in water	903:931	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	5	6	theme	irradiation	834:844	arg1	dose					820:823	the appropriate dose	804:823	the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films	804:897	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	5	6	theme	irradiation	834:844	arg1	30 kGy					937:942	30 kGy	937:942	30 kGy	937:942	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	1	7	theme	poly	259:262	arg1	AG					307:308	AG	307:308	AG	307:308	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	1	7	theme	poly	259:262	arg1	alginate					297:304	poly (vinyl pyrrolidone) (PVP)/sodium alginate	259:304	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs)	259:358	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	0	8	theme	poly	118:121	arg1	NPs					177:179	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	118:179	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	118:179	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	3	9	theme	x-ray	638:642	arg1	diffraction					644:654	x-ray diffraction	638:654	x-ray diffraction (XRD)	638:660	In addition, the structure-property behavior of the copolymers was characterized by IR spectroscopy, thermogravimetric analysis (TGA) and x-ray diffraction (XRD).					
36801218	3	9	theme	x-ray	638:642	arg1	XRD					657:659	XRD	657:659	XRD	657:659	In addition, the structure-property behavior of the copolymers was characterized by IR spectroscopy, thermogravimetric analysis (TGA) and x-ray diffraction (XRD).					
36801218	0	10	theme	NPs	177:179	arg1	copolymerization					98:113	gamma-radiation copolymerization	82:113	gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	82:179	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	4	11	theme	NPs	715:717	arg1	copolymers					719:728	PVP/AG/silver NPs copolymers	701:728	PVP/AG/silver NPs copolymers	701:728	The drug uptake-release characters of PVP/AG/silver NPs copolymers, taking Prednisolone as a model drug, were studied.					
36801218	5	12	from	swelling	915:922	arg1	water					927:931	water	927:931	water	927:931	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	2	13	theme	PVP/AG/Ag	457:465	arg1	copolymers					471:480	PVP/AG/Ag NPs copolymers	457:480	PVP/AG/Ag NPs copolymers	457:480	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	0	14	theme	/silver	169:175	arg1	NPs					177:179	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	118:179	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	118:179	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	5	15	from	dose	820:823	arg1	water					927:931	water	927:931	water	927:931	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	4	16	theme	PVP/AG/silver	701:713	arg1	copolymers					719:728	PVP/AG/silver NPs copolymers	701:728	PVP/AG/silver NPs copolymers	701:728	The drug uptake-release characters of PVP/AG/silver NPs copolymers, taking Prednisolone as a model drug, were studied.					
36801218	1	17	theme	vinyl	265:269	arg1	poly					259:262	poly	259:262	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs)	259:358	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	1	17	theme	vinyl	265:269	arg1	pyrrolidone					271:281	vinyl pyrrolidone	265:281	vinyl pyrrolidone	265:281	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	0	18	theme	vinyl	124:128	arg1	poly					118:121	poly	118:121	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	118:179	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	0	18	theme	vinyl	124:128	arg1	pyrrolidone					130:140	vinyl pyrrolidone	124:140	vinyl pyrrolidone	124:140	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	6	19	theme	Ag	992:993	arg1	NPs					995:997	Ag NPs	992:997	Ag NPs	992:997	The introduction of Ag NPs up to 5 wt% improved the physical properties and enhanced the drug uptake-release characters.					
36801218	2	20	theme	NPs	399:401	arg1	content					403:409	Ag NPs content	396:409	Ag NPs content	396:409	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	1	21	theme	silver	330:335	arg1	NPs					355:357	Ag NPs	352:357	Ag NPs	352:357	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	1	21	theme	silver	330:335	arg1	nanoparticles					337:349	silver nanoparticles	330:349	silver nanoparticles (Ag NPs)	330:358	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	4	22	theme	copolymers	719:728	arg1	characters					687:696	The drug uptake-release characters	663:696	The drug uptake-release characters	663:696	The drug uptake-release characters of PVP/AG/silver NPs copolymers, taking Prednisolone as a model drug, were studied.					
36801218	6	23	theme	5 wt	1005:1008	arg1	%					1009:1009	5 wt%	1005:1009	5 wt%	1005:1009	The introduction of Ag NPs up to 5 wt% improved the physical properties and enhanced the drug uptake-release characters.					
36801218	2	24	theme	Ag	396:397	arg1	content					403:409	Ag NPs content	396:409	Ag NPs content	396:409	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	1	25	theme	nanoparticles	337:349	arg1	presence					318:325	the presence	314:325	the presence of silver nanoparticles (Ag NPs)	314:358	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	4	26	theme	uptake-release	672:685	arg1	characters					687:696	The drug uptake-release characters	663:696	The drug uptake-release characters	663:696	The drug uptake-release characters of PVP/AG/silver NPs copolymers, taking Prednisolone as a model drug, were studied.					
36801218	4	27	theme	drug	667:670	arg1	characters					687:696	The drug uptake-release characters	663:696	The drug uptake-release characters	663:696	The drug uptake-release characters of PVP/AG/silver NPs copolymers, taking Prednisolone as a model drug, were studied.					
36801218	3	28	theme	thermogravimetric	601:617	arg1	TGA					629:631	TGA	629:631	TGA	629:631	In addition, the structure-property behavior of the copolymers was characterized by IR spectroscopy, thermogravimetric analysis (TGA) and x-ray diffraction (XRD).					
36801218	3	28	theme	thermogravimetric	601:617	arg1	analysis					619:626	thermogravimetric analysis	601:626	thermogravimetric analysis (TGA)	601:632	In addition, the structure-property behavior of the copolymers was characterized by IR spectroscopy, thermogravimetric analysis (TGA) and x-ray diffraction (XRD).					
36801218	0	29	theme	delivery	26:33	arg1	characters					35:44	drug delivery characters	21:44	drug delivery characters	21:44	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	5	30	dep	dose	820:823	arg1	achieve					849:855	achieve	849:855	to achieve homogeneous nanocomposites hydrogel films	846:897	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	2	31	theme	NPs	467:469	arg1	copolymers					471:480	PVP/AG/Ag NPs copolymers	457:480	PVP/AG/Ag NPs copolymers	457:480	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	0	32	theme	drug	21:24	arg1	characters					35:44	drug delivery characters	21:44	drug delivery characters	21:44	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	6	33	theme	uptake-release	1066:1079	arg1	characters					1081:1090	the drug uptake-release characters	1057:1090	the drug uptake-release characters	1057:1090	The introduction of Ag NPs up to 5 wt% improved the physical properties and enhanced the drug uptake-release characters.					
36801218	1	34	theme	Nanocomposite	182:194	arg1	hydrogels					196:204	Nanocomposite hydrogels	182:204	Nanocomposite hydrogels	182:204	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	1	35	theme	Ag	352:353	arg1	NPs					355:357	Ag NPs	352:357	Ag NPs	352:357	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	1	35	theme	Ag	352:353	arg1	nanoparticles					337:349	silver nanoparticles	330:349	silver nanoparticles (Ag NPs)	330:358	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	2	36	theme	gel	418:420	arg1	content					422:428	gel content	418:428	gel content	418:428	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	2	37	theme	copolymers	471:480	arg1	content					422:428	gel content	418:428	gel content	418:428	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	2	37	theme	copolymers	471:480	arg1	characters					443:452	swelling characters	434:452	swelling characters	434:452	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	3	38	theme	IR	584:585	arg1	spectroscopy					587:598	IR spectroscopy	584:598	IR spectroscopy	584:598	In addition, the structure-property behavior of the copolymers was characterized by IR spectroscopy, thermogravimetric analysis (TGA) and x-ray diffraction (XRD).					
36801218	2	39	theme	dose	387:390	arg1	effect					365:370	The effect	361:370	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers	361:480	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	0	40	theme	hydrogels	63:71	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	0	40	theme	hydrogels	63:71	arg1	characters					35:44	drug delivery characters	21:44	drug delivery characters	21:44	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	1	41	theme	/sodium	289:295	arg1	AG					307:308	AG	307:308	AG	307:308	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	1	41	theme	/sodium	289:295	arg1	alginate					297:304	poly (vinyl pyrrolidone) (PVP)/sodium alginate	259:304	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs)	259:358	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	0	42	theme	/sodium	148:154	arg1	NPs					177:179	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	118:179	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	118:179	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	3	43	theme	structure-property	517:534	arg1	behavior					536:543	the structure-property behavior	513:543	the structure-property behavior of the copolymers	513:561	In addition, the structure-property behavior of the copolymers was characterized by IR spectroscopy, thermogravimetric analysis (TGA) and x-ray diffraction (XRD).					
36801218	6	44	theme	drug	1061:1064	arg1	characters					1081:1090	the drug uptake-release characters	1057:1090	the drug uptake-release characters	1057:1090	The introduction of Ag NPs up to 5 wt% improved the physical properties and enhanced the drug uptake-release characters.					
36801218	0	45	theme	nanocomposite	49:61	arg1	hydrogels					63:71	nanocomposite hydrogels	49:71	nanocomposite hydrogels	49:71	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	1	46	theme	alginate	297:304	arg1	copolymerization					239:254	copolymerization	239:254	copolymerization	239:254	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
36801218	5	47	theme	hydrogel	884:891	arg1	films					893:897	homogeneous nanocomposites hydrogel films	857:897	homogeneous nanocomposites hydrogel films	857:897	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	4	48	theme	model	756:760	arg1	drug					762:765	a model drug	754:765	a model drug	754:765	The drug uptake-release characters of PVP/AG/silver NPs copolymers, taking Prednisolone as a model drug, were studied.					
36801218	4	48	theme	model	756:760	arg1	Prednisolone					738:749	Prednisolone	738:749	Prednisolone	738:749	The drug uptake-release characters of PVP/AG/silver NPs copolymers, taking Prednisolone as a model drug, were studied.					
36801218	5	49	theme	homogeneous	857:867	arg1	films					893:897	homogeneous nanocomposites hydrogel films	857:897	homogeneous nanocomposites hydrogel films	857:897	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	5	50	theme	appropriate	808:818	arg1	dose					820:823	the appropriate dose	804:823	the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films	804:897	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	5	50	theme	appropriate	808:818	arg1	30 kGy					937:942	30 kGy	937:942	30 kGy	937:942	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	0	51	theme	alginate	156:163	arg1	NPs					177:179	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	118:179	poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs	118:179	Characterization and drug delivery characters of nanocomposite hydrogels based on gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG)/silver NPs.					
36801218	5	52	theme	highest	907:913	arg1	swelling					915:922	the highest swelling	903:922	the highest swelling in water	903:931	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	5	52	theme	highest	907:913	arg1	30 kGy					937:942	30 kGy	937:942	30 kGy	937:942	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	5	53	theme	nanocomposites	869:882	arg1	films					893:897	homogeneous nanocomposites hydrogel films	857:897	homogeneous nanocomposites hydrogel films	857:897	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposites hydrogel films and the highest swelling in water was 30 kGy, regardless of composition.					
36801218	2	54	theme	swelling	434:441	arg1	characters					443:452	swelling characters	434:452	swelling characters	434:452	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	3	55	theme	copolymers	552:561	arg1	behavior					536:543	the structure-property behavior	513:543	the structure-property behavior of the copolymers	513:561	In addition, the structure-property behavior of the copolymers was characterized by IR spectroscopy, thermogravimetric analysis (TGA) and x-ray diffraction (XRD).					
36801218	6	56	theme	NPs	995:997	arg1	introduction					976:987	The introduction	972:987	The introduction of Ag NPs up to 5 wt%	972:1009	The introduction of Ag NPs up to 5 wt% improved the physical properties and enhanced the drug uptake-release characters.					
36801218	2	57	theme	content	403:409	arg1	effect					365:370	The effect	361:370	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers	361:480	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	2	58	theme	irradiation	375:385	arg1	dose					387:390	irradiation dose	375:390	irradiation dose	375:390	The effect of irradiation dose and Ag NPs content on the gel content and swelling characters of PVP/AG/Ag NPs copolymers was investigated.					
36801218	6	59	theme	physical	1024:1031	arg1	properties					1033:1042	the physical properties	1020:1042	the physical properties	1020:1042	The introduction of Ag NPs up to 5 wt% improved the physical properties and enhanced the drug uptake-release characters.					
36801218	1	60	theme	copolymerization	239:254	arg1	gamma-radiation					223:237	gamma-radiation	223:237	gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs)	223:358	Nanocomposite hydrogels were prepared by gamma-radiation copolymerization of poly (vinyl pyrrolidone) (PVP)/sodium alginate (AG) in the presence of silver nanoparticles (Ag NPs).					
35940142	0	0	theme	Pickering	115:123	arg1	emulsion					125:132	Pickering emulsion	115:132	Pickering emulsion	115:132	A resilient and lightweight cellulose/graphene oxide/polymer-derived multifunctional carbon aerogel generated from Pickering emulsion toward a wearable pressure sensor.					
35940142	6	1	theme	conversion	1294:1303	arg1	performance					1305:1315	photothermal conversion performance	1281:1315	photothermal conversion performance	1281:1315	Moreover, the carbon aerogel reveals effective thermal insulation and photothermal conversion performance.					
35940142	7	2	with	aerogels	1412:1419	arg1	functions					1435:1443	multiple functions	1426:1443	multiple functions	1426:1443	These results suggest the great potentials for developing lightweight and compressible carbon aerogels with multiple functions to meet various applications.					
35940142	2	3	theme	multifunctional	388:402	arg1	aerogel					411:417	multifunctional carbon aerogel	388:417	multifunctional carbon aerogel	388:417	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	7	4	theme	multiple	1426:1433	arg1	functions					1435:1443	multiple functions	1426:1443	multiple functions	1426:1443	These results suggest the great potentials for developing lightweight and compressible carbon aerogels with multiple functions to meet various applications.					
35940142	2	5	theme	emulsion	515:522	arg1	gel					524:526	co-stabilized Pickering emulsion gel	491:526	co-stabilized Pickering emulsion gel	491:526	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	5	6	theme	desirable	958:966	arg1	17.65 kPa-1					981:991	17.65 kPa-1	981:991	17.65 kPa-1	981:991	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	5	6	theme	desirable	958:966	arg1	sensitivity					968:978	desirable sensitivity	958:978	desirable sensitivity (17.65 kPa-1)	958:992	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	4	7	dep	%	821:821	arg1	99 					818:820	99 	818:820	99 	818:820	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	7	8	theme	carbon	1405:1410	arg1	aerogels					1412:1419	lightweight and compressible carbon aerogels	1376:1419	lightweight and compressible carbon aerogels with multiple functions	1376:1443	These results suggest the great potentials for developing lightweight and compressible carbon aerogels with multiple functions to meet various applications.					
35940142	5	9	link	styrene-derived	898:912	arg1	-based					934:939	-based	934:939	-based	934:939	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	5	10	theme	pressure	1023:1030	arg1	17.65 kPa-1					981:991	17.65 kPa-1	981:991	17.65 kPa-1	981:991	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	5	10	theme	pressure	1023:1030	arg1	60 Pa					1033:1037	60 Pa	1033:1037	60 Pa	1033:1037	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	5	10	theme	pressure	1023:1030	arg1	time					1061:1064	fast responsive time	1045:1064	fast responsive time (130 ms)	1045:1073	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	5	10	theme	pressure	1023:1030	arg1	sensitivity					968:978	desirable sensitivity	958:978	desirable sensitivity (17.65 kPa-1)	958:992	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	5	10	theme	pressure	1023:1030	arg1	130 ms					1067:1072	130 ms	1067:1072	130 ms	1067:1072	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	5	10	theme	pressure	1023:1030	arg1	limit					1014:1018	ultralow detection limit	995:1018	ultralow detection limit of pressure (60 Pa)	995:1038	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	4	11	theme	compression	793:803	arg1	strain					805:810	high compression strain	788:810	high compression strain up to 99 %	788:821	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	4	12	theme	high	788:791	arg1	strain					805:810	high compression strain	788:810	high compression strain up to 99 %	788:821	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	0	13	theme	wearable	143:150	arg1	sensor					161:166	a wearable pressure sensor	141:166	a wearable pressure sensor	141:166	A resilient and lightweight cellulose/graphene oxide/polymer-derived multifunctional carbon aerogel generated from Pickering emulsion toward a wearable pressure sensor.					
35940142	3	14	theme	resulting	572:580	arg1	aerogel					589:595	The resulting carbon aerogel	568:595	The resulting carbon aerogel	568:595	The resulting carbon aerogel exhibits tunable mechanical, hydrophilic and hydrophobic properties due to varying the elemental composition and the pyrolysis of introduced polymers.					
35940142	5	15	theme	-based	934:939	arg1	sensor					941:946	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor	863:946	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor	863:946	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	1	16	theme	competitive	187:197	arg1	electronics					205:215	competitive smart electronics	187:215	competitive smart electronics	187:215	In the new era of competitive smart electronics, the development of compressible multifunctional carbon aerogels is highly needed, but still faces enormous challenges.					
35940142	2	17	theme	high-temperature	540:555	arg1	annealing					557:565	high-temperature annealing	540:565	high-temperature annealing	540:565	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	5	18	theme	spatial	1134:1140	arg1	distribution					1151:1162	spatial pressure distribution	1134:1162	spatial pressure distribution	1134:1162	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	3	19	theme	carbon	582:587	arg1	aerogel					589:595	The resulting carbon aerogel	568:595	The resulting carbon aerogel	568:595	The resulting carbon aerogel exhibits tunable mechanical, hydrophilic and hydrophobic properties due to varying the elemental composition and the pyrolysis of introduced polymers.					
35940142	1	20	theme	smart	199:203	arg1	electronics					205:215	competitive smart electronics	187:215	competitive smart electronics	187:215	In the new era of competitive smart electronics, the development of compressible multifunctional carbon aerogels is highly needed, but still faces enormous challenges.					
35940142	0	21	theme	resilient	2:10	arg1	cellulose/graphene					28:45	A resilient and lightweight cellulose/graphene	0:45	A resilient and lightweight cellulose/graphene	0:45	A resilient and lightweight cellulose/graphene oxide/polymer-derived multifunctional carbon aerogel generated from Pickering emulsion toward a wearable pressure sensor.					
35940142	5	22	theme	pressure	1142:1149	arg1	distribution					1151:1162	spatial pressure distribution	1134:1162	spatial pressure distribution	1134:1162	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	5	23	theme	ultralow	995:1002	arg1	limit					1014:1018	ultralow detection limit	995:1018	ultralow detection limit of pressure (60 Pa)	995:1038	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	5	23	theme	ultralow	995:1002	arg1	60 Pa					1033:1037	60 Pa	1033:1037	60 Pa	1033:1037	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	1	24	theme	electronics	205:215	arg1	era					180:182	the new era	172:182	the new era of competitive smart electronics	172:215	In the new era of competitive smart electronics, the development of compressible multifunctional carbon aerogels is highly needed, but still faces enormous challenges.					
35940142	2	25	theme	oxide	480:484	arg1	freeze-drying					423:435	freeze-drying	423:435	freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing	423:565	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	2	26	dep	oxide	480:484	arg1	GO					487:488	GO	487:488	GO	487:488	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	3	27	theme	hydrophilic	626:636	arg1	properties					654:663	tunable mechanical, hydrophilic and hydrophobic properties	606:663	tunable mechanical, hydrophilic and hydrophobic properties	606:663	The resulting carbon aerogel exhibits tunable mechanical, hydrophilic and hydrophobic properties due to varying the elemental composition and the pyrolysis of introduced polymers.					
35940142	7	28	theme	compressible	1392:1403	arg1	aerogels					1412:1419	lightweight and compressible carbon aerogels	1376:1419	lightweight and compressible carbon aerogels with multiple functions	1376:1443	These results suggest the great potentials for developing lightweight and compressible carbon aerogels with multiple functions to meet various applications.					
35940142	0	29	theme	lightweight	16:26	arg1	cellulose/graphene					28:45	A resilient and lightweight cellulose/graphene	0:45	A resilient and lightweight cellulose/graphene	0:45	A resilient and lightweight cellulose/graphene oxide/polymer-derived multifunctional carbon aerogel generated from Pickering emulsion toward a wearable pressure sensor.					
35940142	6	30	theme	thermal	1258:1264	arg1	insulation					1266:1275	effective thermal insulation	1248:1275	effective thermal insulation	1248:1275	Moreover, the carbon aerogel reveals effective thermal insulation and photothermal conversion performance.					
35940142	3	31	theme	tunable	606:612	arg1	properties					654:663	tunable mechanical, hydrophilic and hydrophobic properties	606:663	tunable mechanical, hydrophilic and hydrophobic properties	606:663	The resulting carbon aerogel exhibits tunable mechanical, hydrophilic and hydrophobic properties due to varying the elemental composition and the pyrolysis of introduced polymers.					
35940142	5	32	theme	detection	1004:1012	arg1	limit					1014:1018	ultralow detection limit	995:1018	ultralow detection limit of pressure (60 Pa)	995:1038	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	5	32	theme	detection	1004:1012	arg1	60 Pa					1033:1037	60 Pa	1033:1037	60 Pa	1033:1037	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	1	33	theme	enormous	316:323	arg1	challenges					325:334	enormous challenges	316:334	enormous challenges	316:334	In the new era of competitive smart electronics, the development of compressible multifunctional carbon aerogels is highly needed, but still faces enormous challenges.					
35940142	0	34	theme	pressure	152:159	arg1	sensor					161:166	a wearable pressure sensor	141:166	a wearable pressure sensor	141:166	A resilient and lightweight cellulose/graphene oxide/polymer-derived multifunctional carbon aerogel generated from Pickering emulsion toward a wearable pressure sensor.					
35940142	3	35	theme	mechanical	614:623	arg1	properties					654:663	tunable mechanical, hydrophilic and hydrophobic properties	606:663	tunable mechanical, hydrophilic and hydrophobic properties	606:663	The resulting carbon aerogel exhibits tunable mechanical, hydrophilic and hydrophobic properties due to varying the elemental composition and the pyrolysis of introduced polymers.					
35940142	7	36	theme	lightweight	1376:1386	arg1	aerogels					1412:1419	lightweight and compressible carbon aerogels	1376:1419	lightweight and compressible carbon aerogels with multiple functions	1376:1443	These results suggest the great potentials for developing lightweight and compressible carbon aerogels with multiple functions to meet various applications.					
35940142	2	37	theme	Pickering	505:513	arg1	gel					524:526	co-stabilized Pickering emulsion gel	491:526	co-stabilized Pickering emulsion gel	491:526	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	3	38	theme	introduced	727:736	arg1	polymers					738:745	introduced polymers	727:745	introduced polymers	727:745	The resulting carbon aerogel exhibits tunable mechanical, hydrophilic and hydrophobic properties due to varying the elemental composition and the pyrolysis of introduced polymers.					
35940142	6	39	theme	photothermal	1281:1292	arg1	performance					1305:1315	photothermal conversion performance	1281:1315	photothermal conversion performance	1281:1315	Moreover, the carbon aerogel reveals effective thermal insulation and photothermal conversion performance.					
35940142	6	40	theme	carbon	1225:1230	arg1	aerogel					1232:1238	the carbon aerogel	1221:1238	the carbon aerogel	1221:1238	Moreover, the carbon aerogel reveals effective thermal insulation and photothermal conversion performance.					
35940142	2	41	theme	cellulose	440:448	arg1	CNF					462:464	CNF	462:464	CNF	462:464	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	2	41	theme	cellulose	440:448	arg1	nanofibers					450:459	cellulose nanofibers	440:459	cellulose nanofibers (CNF)	440:465	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	4	42	contain	has	827:829	arg1	aerogel					759:765	The carbon aerogel	748:765	The carbon aerogel	748:765	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	4	42	contain	has	827:829	arg2	density					840:846	ultralow density	831:846	ultralow density (1.82 mg/cm3)	831:860	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	4	42	contain	has	827:829	arg1	resilient					770:778	resilient	770:778	resilient	770:778	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	4	42	contain	has	827:829	arg2	1.82 mg/cm3					849:859	1.82 mg/cm3	849:859	1.82 mg/cm3	849:859	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	2	43	theme	co-stabilized	491:503	arg1	gel					524:526	co-stabilized Pickering emulsion gel	491:526	co-stabilized Pickering emulsion gel	491:526	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	5	44	theme	human	1106:1110	arg1	activity					1112:1119	human activity	1106:1119	human activity	1106:1119	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	2	45	theme	graphene	471:478	arg1	oxide					480:484	graphene oxide	471:484	graphene oxide (GO)	471:489	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	7	46	theme	various	1453:1459	arg1	applications					1461:1472	various applications	1453:1472	various applications	1453:1472	These results suggest the great potentials for developing lightweight and compressible carbon aerogels with multiple functions to meet various applications.					
35940142	1	47	theme	compressible	237:248	arg1	aerogels					273:280	compressible multifunctional carbon aerogels	237:280	compressible multifunctional carbon aerogels	237:280	In the new era of competitive smart electronics, the development of compressible multifunctional carbon aerogels is highly needed, but still faces enormous challenges.					
35940142	0	48	theme	carbon	85:90	arg1	aerogel					92:98	multifunctional carbon aerogel	69:98	multifunctional carbon aerogel generated from Pickering emulsion toward a wearable pressure sensor	69:166	A resilient and lightweight cellulose/graphene oxide/polymer-derived multifunctional carbon aerogel generated from Pickering emulsion toward a wearable pressure sensor.					
35940142	4	49	dep	99 	818:820	arg1	to					815:816	to	815:816	to	815:816	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	0	50	theme	multifunctional	69:83	arg1	aerogel					92:98	multifunctional carbon aerogel	69:98	multifunctional carbon aerogel generated from Pickering emulsion toward a wearable pressure sensor	69:166	A resilient and lightweight cellulose/graphene oxide/polymer-derived multifunctional carbon aerogel generated from Pickering emulsion toward a wearable pressure sensor.					
35940142	3	51	theme	polymers	738:745	arg1	composition					694:704	the elemental composition	680:704	the elemental composition	680:704	The resulting carbon aerogel exhibits tunable mechanical, hydrophilic and hydrophobic properties due to varying the elemental composition and the pyrolysis of introduced polymers.					
35940142	3	51	theme	polymers	738:745	arg1	pyrolysis					714:722	the pyrolysis	710:722	the pyrolysis of introduced polymers	710:745	The resulting carbon aerogel exhibits tunable mechanical, hydrophilic and hydrophobic properties due to varying the elemental composition and the pyrolysis of introduced polymers.					
35940142	3	52	theme	hydrophobic	642:652	arg1	properties					654:663	tunable mechanical, hydrophilic and hydrophobic properties	606:663	tunable mechanical, hydrophilic and hydrophobic properties	606:663	The resulting carbon aerogel exhibits tunable mechanical, hydrophilic and hydrophobic properties due to varying the elemental composition and the pyrolysis of introduced polymers.					
35940142	7	53	theme	great	1344:1348	arg1	potentials					1350:1359	the great potentials	1340:1359	the great potentials for developing lightweight and compressible carbon aerogels with multiple functions to meet various applications	1340:1472	These results suggest the great potentials for developing lightweight and compressible carbon aerogels with multiple functions to meet various applications.					
35940142	5	54	theme	responsive	1050:1059	arg1	130 ms					1067:1072	130 ms	1067:1072	130 ms	1067:1072	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	5	54	theme	responsive	1050:1059	arg1	time					1061:1064	fast responsive time	1045:1064	fast responsive time (130 ms)	1045:1073	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	6	55	theme	effective	1248:1256	arg1	insulation					1266:1275	effective thermal insulation	1248:1275	effective thermal insulation	1248:1275	Moreover, the carbon aerogel reveals effective thermal insulation and photothermal conversion performance.					
35940142	2	56	dep	nanofibers	450:459	arg1	gel					524:526	co-stabilized Pickering emulsion gel	491:526	co-stabilized Pickering emulsion gel	491:526	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	2	57	theme	robust	359:364	arg1	strategy					366:373	a robust strategy	357:373	a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing	357:565	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	1	58	theme	multifunctional	250:264	arg1	aerogels					273:280	compressible multifunctional carbon aerogels	237:280	compressible multifunctional carbon aerogels	237:280	In the new era of competitive smart electronics, the development of compressible multifunctional carbon aerogels is highly needed, but still faces enormous challenges.					
35940142	4	59	theme	ultralow	831:838	arg1	1.82 mg/cm3					849:859	1.82 mg/cm3	849:859	1.82 mg/cm3	849:859	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	4	59	theme	ultralow	831:838	arg1	density					840:846	ultralow density	831:846	ultralow density (1.82 mg/cm3)	831:860	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	3	60	theme	elemental	684:692	arg1	composition					694:704	the elemental composition	680:704	the elemental composition	680:704	The resulting carbon aerogel exhibits tunable mechanical, hydrophilic and hydrophobic properties due to varying the elemental composition and the pyrolysis of introduced polymers.					
35940142	2	61	theme	carbon	404:409	arg1	aerogel					411:417	multifunctional carbon aerogel	388:417	multifunctional carbon aerogel	388:417	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	1	62	theme	carbon	266:271	arg1	aerogels					273:280	compressible multifunctional carbon aerogels	237:280	compressible multifunctional carbon aerogels	237:280	In the new era of competitive smart electronics, the development of compressible multifunctional carbon aerogels is highly needed, but still faces enormous challenges.					
35940142	2	63	theme	nanofibers	450:459	arg1	freeze-drying					423:435	freeze-drying	423:435	freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing	423:565	Here we demonstrate a robust strategy to fabricate multifunctional carbon aerogel via freeze-drying of cellulose nanofibers (CNF) and graphene oxide (GO) co-stabilized Pickering emulsion gel followed by high-temperature annealing.					
35940142	5	64	theme	fast	1045:1048	arg1	130 ms					1067:1072	130 ms	1067:1072	130 ms	1067:1072	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	5	64	theme	fast	1045:1048	arg1	time					1061:1064	fast responsive time	1045:1064	fast responsive time (130 ms)	1045:1073	The CNF/GO/acrylonitrile butadiene styrene-derived carbon aerogel (CRA)-based sensor has shown desirable sensitivity (17.65 kPa-1), ultralow detection limit of pressure (60 Pa), and fast responsive time (130 ms), which is capable of detecting human activity, identifying spatial pressure distribution, and communicating with smartphones via Wi-Fi.					
35940142	4	65	theme	carbon	752:757	arg1	aerogel					759:765	The carbon aerogel	748:765	The carbon aerogel	748:765	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	4	65	theme	carbon	752:757	arg1	resilient					770:778	resilient	770:778	resilient	770:778	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	1	66	theme	aerogels	273:280	arg1	development					222:232	the development	218:232	the development of compressible multifunctional carbon aerogels	218:280	In the new era of competitive smart electronics, the development of compressible multifunctional carbon aerogels is highly needed, but still faces enormous challenges.					
35940142	4	67	dep	strain	805:810	arg1	%					821:821	%	821:821	%	821:821	The carbon aerogel is resilient against high compression strain up to 99 % and has ultralow density (1.82 mg/cm3).					
35940142	1	68	theme	new	176:178	arg1	era					180:182	the new era	172:182	the new era of competitive smart electronics	172:215	In the new era of competitive smart electronics, the development of compressible multifunctional carbon aerogels is highly needed, but still faces enormous challenges.					
37039647	10	0	theme	host	1838:1841	arg1	defenses					1843:1850	host defenses	1838:1850	host defenses	1838:1850	These changes are anticipated to enable the subcuticular differentiation of runner hyphae and stromata by V. inaequalis, as well as to protect these structures from host defenses and recognition by the host immune system.					
37039647	14	1	theme	important	2329:2337	arg1	scab					2301:2304	scab	2301:2304	scab	2301:2304	This is typified by Venturia inaequalis, which causes scab, the most economically important disease of apples.					
37039647	14	1	theme	important	2329:2337	arg1	disease					2339:2345	the most economically important disease	2307:2345	the most economically important disease of apples	2307:2355	This is typified by Venturia inaequalis, which causes scab, the most economically important disease of apples.					
37039647	1	2	theme	apples	244:249	arg1	disease					233:239	the most economically important disease	201:239	the most economically important disease of apples	201:249	Scab, caused by the biotrophic fungal pathogen Venturia inaequalis, is the most economically important disease of apples.					
37039647	1	2	theme	apples	244:249	arg1	Scab					130:133	Scab	130:133	Scab	130:133	Scab, caused by the biotrophic fungal pathogen Venturia inaequalis, is the most economically important disease of apples.					
37039647	6	3	theme	linkage	882:888	arg1	analysis					890:897	a glycosidic linkage analysis	869:897	a glycosidic linkage analysis of sporulating tubular hyphae	869:927	To better understand these processes, we first performed a glycosidic linkage analysis of sporulating tubular hyphae from V. inaequalis developed in culture.					
37039647	9	4	theme	hyphae	1582:1587	arg1	surface					1564:1570	the surface	1560:1570	the surface of runner hyphae and stromata	1560:1600	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	10	5	theme	host	1875:1878	arg1	system					1887:1892	the host immune system	1871:1892	the host immune system	1871:1892	These changes are anticipated to enable the subcuticular differentiation of runner hyphae and stromata by V. inaequalis, as well as to protect these structures from host defenses and recognition by the host immune system.					
37039647	11	6	theme	food	1951:1954	arg1	security					1956:1963	food security	1951:1963	food security	1951:1963	IMPORTANCE Plant-pathogenic fungi are a major threat to food security.					
37039647	9	7	theme	stromata	1593:1600	arg1	surface					1564:1570	the surface	1560:1570	the surface of runner hyphae and stromata	1560:1600	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	16	8	theme	host	2789:2792	arg1	colonization					2794:2805	subcuticular host colonization	2776:2805	subcuticular host colonization by other plant-pathogenic fungi	2776:2837	These changes are anticipated to enable host colonization by V. inaequalis and provide a foundation for understanding subcuticular host colonization by other plant-pathogenic fungi.					
37039647	0	9	theme	Cell	0:3	arg1	Dynamics					23:30	Cell Wall Carbohydrate Dynamics	0:30	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.	0:128	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.					
37039647	1	10	theme	fungal	161:166	arg1	pathogen					168:175	the biotrophic fungal pathogen	146:175	the biotrophic fungal pathogen	146:175	Scab, caused by the biotrophic fungal pathogen Venturia inaequalis, is the most economically important disease of apples.					
37039647	2	11	theme	subcuticular	298:309	arg1	environment					316:326	the subcuticular host environment	294:326	the subcuticular host environment	294:326	During infection, V. inaequalis colonizes the subcuticular host environment, where it develops specialized infection structures called runner hyphae and stromata.					
37039647	9	12	theme	V.	1381:1382	arg1	inaequalis					1384:1393	V. inaequalis	1381:1393	V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan,	1381:1500	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	17	13	theme	subcuticular	2945:2956	arg1	fungi					2975:2979	subcuticular plant-pathogenic fungi	2945:2979	subcuticular plant-pathogenic fungi	2945:2979	Such an understanding is important, as it may inform the development of novel control strategies against subcuticular plant-pathogenic fungi.					
37039647	16	14	theme	plant-pathogenic	2816:2831	arg1	fungi					2833:2837	other plant-pathogenic fungi	2810:2837	other plant-pathogenic fungi	2810:2837	These changes are anticipated to enable host colonization by V. inaequalis and provide a foundation for understanding subcuticular host colonization by other plant-pathogenic fungi.					
37039647	8	15	theme	laser	1182:1186	arg1	microscopy					1197:1206	confocal laser scanning microscopy	1173:1206	confocal laser scanning microscopy	1173:1206	Next, we used transcriptomics and confocal laser scanning microscopy to provide insights into the cell wall carbohydrate composition of runner hyphae and stromata.					
37039647	0	16	theme	Venturia	109:116	arg1	Fungus					101:106	the Apple Scab Fungus	86:106	the Apple Scab Fungus	86:106	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.					
37039647	0	16	theme	Venturia	109:116	arg1	inaequalis					118:127	Venturia inaequalis	109:127	Venturia inaequalis	109:127	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.					
37039647	9	17	theme	subcuticular	1340:1351	arg1	colonization					1358:1369	subcuticular host colonization	1340:1369	subcuticular host colonization	1340:1369	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	10	18	theme	hyphae	1756:1761	arg1	differentiation					1730:1744	the subcuticular differentiation	1713:1744	the subcuticular differentiation of runner hyphae and stromata by V. inaequalis	1713:1791	These changes are anticipated to enable the subcuticular differentiation of runner hyphae and stromata by V. inaequalis, as well as to protect these structures from host defenses and recognition by the host immune system.					
37039647	7	19	theme	V.	1002:1003	arg1	wall					1021:1024	the V. inaequalis cell wall	998:1024	the V. inaequalis cell wall	998:1024	This analysis revealed that the V. inaequalis cell wall is mostly composed of glucans (44%) and mannans (37%), whereas chitin represents a much smaller proportion (4%).					
37039647	9	20	theme	carbohydrate	1650:1661	arg1	chitosan					1663:1670	the less-immunogenic carbohydrate chitosan	1629:1670	the less-immunogenic carbohydrate chitosan	1629:1670	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	3	21	theme	infection	572:580	arg1	cycle					582:586	the infection cycle	568:586	the infection cycle	568:586	These structures are thought to be involved in nutrient acquisition and effector (virulence factor) delivery, but also give rise to conidia that further the infection cycle.					
37039647	6	22	theme	tubular	914:920	arg1	hyphae					922:927	sporulating tubular hyphae	902:927	sporulating tubular hyphae	902:927	To better understand these processes, we first performed a glycosidic linkage analysis of sporulating tubular hyphae from V. inaequalis developed in culture.					
37039647	12	23	theme	latent	2024:2029	arg1	infections					2044:2053	latent asymptomatic infections	2024:2053	latent asymptomatic infections	2024:2053	Among these are subcuticular pathogens, which often cause latent asymptomatic infections, making them difficult to control.					
37039647	10	24	theme	V.	1779:1780	arg1	inaequalis					1782:1791	V. inaequalis	1779:1791	V. inaequalis	1779:1791	These changes are anticipated to enable the subcuticular differentiation of runner hyphae and stromata by V. inaequalis, as well as to protect these structures from host defenses and recognition by the host immune system.					
37039647	9	25	theme	immunogenic	1442:1452	arg1	β-1,6-glucan					1488:1499	β-1,6-glucan	1488:1499	β-1,6-glucan	1488:1499	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	9	25	theme	immunogenic	1442:1452	arg1	chitin					1477:1482	chitin	1477:1482	chitin	1477:1482	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	9	25	theme	immunogenic	1442:1452	arg1	carbohydrates					1454:1466	immunogenic carbohydrates	1442:1466	immunogenic carbohydrates	1442:1466	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	12	26	theme	subcuticular	1982:1993	arg1	pathogens					1995:2003	subcuticular pathogens	1982:2003	subcuticular pathogens	1982:2003	Among these are subcuticular pathogens, which often cause latent asymptomatic infections, making them difficult to control.					
37039647	0	27	theme	Infection	62:70	arg1	Structures					72:81	Infection Structures	62:81	Infection Structures	62:81	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.					
37039647	10	28	theme	stromata	1767:1774	arg1	differentiation					1730:1744	the subcuticular differentiation	1713:1744	the subcuticular differentiation of runner hyphae and stromata by V. inaequalis	1713:1791	These changes are anticipated to enable the subcuticular differentiation of runner hyphae and stromata by V. inaequalis, as well as to protect these structures from host defenses and recognition by the host immune system.					
37039647	8	29	theme	carbohydrate	1247:1258	arg1	composition					1260:1270	the cell wall carbohydrate composition	1233:1270	the cell wall carbohydrate composition of runner hyphae and stromata	1233:1300	Next, we used transcriptomics and confocal laser scanning microscopy to provide insights into the cell wall carbohydrate composition of runner hyphae and stromata.					
37039647	5	30	theme	host	756:759	arg1	defenses					761:768	host defenses	756:768	host defenses	756:768	Likewise, nothing is known about how these structures are protected from host defenses or recognition by the host immune system.					
37039647	0	31	theme	Apple	90:94	arg1	Fungus					101:106	the Apple Scab Fungus	86:106	the Apple Scab Fungus	86:106	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.					
37039647	0	31	theme	Apple	90:94	arg1	inaequalis					118:127	Venturia inaequalis	109:127	Venturia inaequalis	109:127	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.					
37039647	8	32	theme	hyphae	1282:1287	arg1	composition					1260:1270	the cell wall carbohydrate composition	1233:1270	the cell wall carbohydrate composition of runner hyphae and stromata	1233:1300	Next, we used transcriptomics and confocal laser scanning microscopy to provide insights into the cell wall carbohydrate composition of runner hyphae and stromata.					
37039647	17	33	theme	strategies	2926:2935	arg1	development					2897:2907	the development	2893:2907	the development of novel control strategies against subcuticular plant-pathogenic fungi	2893:2979	Such an understanding is important, as it may inform the development of novel control strategies against subcuticular plant-pathogenic fungi.					
37039647	8	34	theme	stromata	1293:1300	arg1	composition					1260:1270	the cell wall carbohydrate composition	1233:1270	the cell wall carbohydrate composition of runner hyphae and stromata	1233:1300	Next, we used transcriptomics and confocal laser scanning microscopy to provide insights into the cell wall carbohydrate composition of runner hyphae and stromata.					
37039647	2	35	theme	runner	387:392	arg1	hyphae					394:399	runner hyphae	387:399	runner hyphae	387:399	During infection, V. inaequalis colonizes the subcuticular host environment, where it develops specialized infection structures called runner hyphae and stromata.					
37039647	5	36	theme	host	792:795	arg1	system					804:809	the host immune system	788:809	the host immune system	788:809	Likewise, nothing is known about how these structures are protected from host defenses or recognition by the host immune system.					
37039647	13	37	theme	specialized	2157:2167	arg1	structures					2192:2201	specialized subcuticular infection structures	2157:2201	specialized subcuticular infection structures that, to date, remain largely understudied	2157:2244	A key feature of these pathogens is their ability to differentiate specialized subcuticular infection structures that, to date, remain largely understudied.					
37039647	3	38	theme	nutrient	462:469	arg1	acquisition					471:481	nutrient acquisition	462:481	nutrient acquisition	462:481	These structures are thought to be involved in nutrient acquisition and effector (virulence factor) delivery, but also give rise to conidia that further the infection cycle.					
37039647	0	39	theme	Carbohydrate	10:21	arg1	Dynamics					23:30	Cell Wall Carbohydrate Dynamics	0:30	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.	0:128	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.					
37039647	13	40	theme	infection	2182:2190	arg1	structures					2192:2201	specialized subcuticular infection structures	2157:2201	specialized subcuticular infection structures that, to date, remain largely understudied	2157:2244	A key feature of these pathogens is their ability to differentiate specialized subcuticular infection structures that, to date, remain largely understudied.					
37039647	9	41	from	growth	1532:1537	arg1	culture					1542:1548	culture	1542:1548	culture	1542:1548	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	8	42	theme	cell	1237:1240	arg1	composition					1260:1270	the cell wall carbohydrate composition	1233:1270	the cell wall carbohydrate composition of runner hyphae and stromata	1233:1300	Next, we used transcriptomics and confocal laser scanning microscopy to provide insights into the cell wall carbohydrate composition of runner hyphae and stromata.					
37039647	7	43	theme	smaller	1114:1120	arg1	proportion					1122:1131	a much smaller proportion	1107:1131	a much smaller proportion (4%)	1107:1136	This analysis revealed that the V. inaequalis cell wall is mostly composed of glucans (44%) and mannans (37%), whereas chitin represents a much smaller proportion (4%).					
37039647	7	43	theme	smaller	1114:1120	arg1	%					1135:1135	4%	1134:1135	4%	1134:1135	This analysis revealed that the V. inaequalis cell wall is mostly composed of glucans (44%) and mannans (37%), whereas chitin represents a much smaller proportion (4%).					
37039647	8	44	used	used	1148:1151	arg2	we					1145:1146	we	1145:1146	we	1145:1146	Next, we used transcriptomics and confocal laser scanning microscopy to provide insights into the cell wall carbohydrate composition of runner hyphae and stromata.					
37039647	7	45	dep	composed	1036:1043	arg1	whereas					1081:1087	whereas	1081:1087	whereas	1081:1087	This analysis revealed that the V. inaequalis cell wall is mostly composed of glucans (44%) and mannans (37%), whereas chitin represents a much smaller proportion (4%).					
37039647	14	46	theme	apples	2350:2355	arg1	scab					2301:2304	scab	2301:2304	scab	2301:2304	This is typified by Venturia inaequalis, which causes scab, the most economically important disease of apples.					
37039647	14	46	theme	apples	2350:2355	arg1	disease					2339:2345	the most economically important disease	2307:2345	the most economically important disease of apples	2307:2355	This is typified by Venturia inaequalis, which causes scab, the most economically important disease of apples.					
37039647	15	47	theme	wall	2517:2520	arg1	β-1,6-glucan					2548:2559	β-1,6-glucan	2548:2559	β-1,6-glucan	2548:2559	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	15	47	theme	wall	2517:2520	arg1	chitin					2537:2542	chitin	2537:2542	chitin	2537:2542	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	15	47	theme	wall	2517:2520	arg1	carbohydrates					2522:2534	two immunogenic cell wall carbohydrates	2496:2534	two immunogenic cell wall carbohydrates	2496:2534	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	13	48	theme	pathogens	2113:2121	arg1	feature					2096:2102	A key feature	2090:2102	A key feature of these pathogens	2090:2121	A key feature of these pathogens is their ability to differentiate specialized subcuticular infection structures that, to date, remain largely understudied.					
37039647	13	48	theme	pathogens	2113:2121	arg1	ability					2132:2138	their ability to differentiate specialized subcuticular infection structures that, to date, remain largely understudied	2126:2244	their ability to differentiate specialized subcuticular infection structures that, to date, remain largely understudied	2126:2244	A key feature of these pathogens is their ability to differentiate specialized subcuticular infection structures that, to date, remain largely understudied.					
37039647	6	49	theme	sporulating	902:912	arg1	hyphae					922:927	sporulating tubular hyphae	902:927	sporulating tubular hyphae	902:927	To better understand these processes, we first performed a glycosidic linkage analysis of sporulating tubular hyphae from V. inaequalis developed in culture.					
37039647	2	50	theme	specialized	347:357	arg1	structures					369:378	specialized infection structures	347:378	specialized infection structures called runner hyphae and stromata	347:412	During infection, V. inaequalis colonizes the subcuticular host environment, where it develops specialized infection structures called runner hyphae and stromata.					
37039647	15	51	theme	immunogenic	2500:2510	arg1	β-1,6-glucan					2548:2559	β-1,6-glucan	2548:2559	β-1,6-glucan	2548:2559	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	15	51	theme	immunogenic	2500:2510	arg1	chitin					2537:2542	chitin	2537:2542	chitin	2537:2542	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	15	51	theme	immunogenic	2500:2510	arg1	carbohydrates					2522:2534	two immunogenic cell wall carbohydrates	2496:2534	two immunogenic cell wall carbohydrates	2496:2534	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	9	52	theme	runner	1575:1580	arg1	hyphae					1582:1587	runner hyphae	1575:1587	runner hyphae	1575:1587	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	9	53	theme	host	1353:1356	arg1	colonization					1358:1369	subcuticular host colonization	1340:1369	subcuticular host colonization	1340:1369	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	6	54	theme	glycosidic	871:880	arg1	analysis					890:897	a glycosidic linkage analysis	869:897	a glycosidic linkage analysis of sporulating tubular hyphae	869:927	To better understand these processes, we first performed a glycosidic linkage analysis of sporulating tubular hyphae from V. inaequalis developed in culture.					
37039647	11	55	theme	major	1935:1939	arg1	fungi					1923:1927	IMPORTANCE Plant-pathogenic fungi	1895:1927	IMPORTANCE Plant-pathogenic fungi	1895:1927	IMPORTANCE Plant-pathogenic fungi are a major threat to food security.					
37039647	11	55	theme	major	1935:1939	arg1	threat					1941:1946	a major threat	1933:1946	a major threat to food security	1933:1963	IMPORTANCE Plant-pathogenic fungi are a major threat to food security.					
37039647	15	56	theme	subcuticular	2576:2587	arg1	structures					2599:2608	its subcuticular infection structures	2572:2608	its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan	2572:2655	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	1	57	theme	biotrophic	150:159	arg1	pathogen					168:175	the biotrophic fungal pathogen	146:175	the biotrophic fungal pathogen	146:175	Scab, caused by the biotrophic fungal pathogen Venturia inaequalis, is the most economically important disease of apples.					
37039647	16	58	theme	subcuticular	2776:2787	arg1	colonization					2794:2805	subcuticular host colonization	2776:2805	subcuticular host colonization by other plant-pathogenic fungi	2776:2837	These changes are anticipated to enable host colonization by V. inaequalis and provide a foundation for understanding subcuticular host colonization by other plant-pathogenic fungi.					
37039647	2	59	theme	host	311:314	arg1	environment					316:326	the subcuticular host environment	294:326	the subcuticular host environment	294:326	During infection, V. inaequalis colonizes the subcuticular host environment, where it develops specialized infection structures called runner hyphae and stromata.					
37039647	9	60	theme	inaequalis	1384:1393	arg1	genes					1372:1376	genes	1372:1376	genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan,	1372:1500	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	10	61	theme	subcuticular	1717:1728	arg1	differentiation					1730:1744	the subcuticular differentiation	1713:1744	the subcuticular differentiation of runner hyphae and stromata by V. inaequalis	1713:1791	These changes are anticipated to enable the subcuticular differentiation of runner hyphae and stromata by V. inaequalis, as well as to protect these structures from host defenses and recognition by the host immune system.					
37039647	16	62	theme	other	2810:2814	arg1	fungi					2833:2837	other plant-pathogenic fungi	2810:2837	other plant-pathogenic fungi	2810:2837	These changes are anticipated to enable host colonization by V. inaequalis and provide a foundation for understanding subcuticular host colonization by other plant-pathogenic fungi.					
37039647	12	63	theme	asymptomatic	2031:2042	arg1	infections					2044:2053	latent asymptomatic infections	2024:2053	latent asymptomatic infections	2024:2053	Among these are subcuticular pathogens, which often cause latent asymptomatic infections, making them difficult to control.					
37039647	3	64	theme	factor	507:512	arg1	delivery					515:522	effector (virulence factor) delivery	487:522	effector (virulence factor) delivery	487:522	These structures are thought to be involved in nutrient acquisition and effector (virulence factor) delivery, but also give rise to conidia that further the infection cycle.					
37039647	10	65	theme	immune	1880:1885	arg1	system					1887:1892	the host immune system	1871:1892	the host immune system	1871:1892	These changes are anticipated to enable the subcuticular differentiation of runner hyphae and stromata by V. inaequalis, as well as to protect these structures from host defenses and recognition by the host immune system.					
37039647	17	66	theme	control	2918:2924	arg1	strategies					2926:2935	novel control strategies	2912:2935	novel control strategies against subcuticular plant-pathogenic fungi	2912:2979	Such an understanding is important, as it may inform the development of novel control strategies against subcuticular plant-pathogenic fungi.					
37039647	9	67	theme	less-immunogenic	1633:1648	arg1	chitosan					1663:1670	the less-immunogenic carbohydrate chitosan	1629:1670	the less-immunogenic carbohydrate chitosan	1629:1670	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	15	68	with	structures	2599:2608	arg1	carbohydrate					2634:2645	a less-immunogenic carbohydrate	2615:2645	a less-immunogenic carbohydrate	2615:2645	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	15	68	with	structures	2599:2608	arg1	chitosan					2648:2655	chitosan	2648:2655	chitosan	2648:2655	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	8	69	theme	scanning	1188:1195	arg1	microscopy					1197:1206	confocal laser scanning microscopy	1173:1206	confocal laser scanning microscopy	1173:1206	Next, we used transcriptomics and confocal laser scanning microscopy to provide insights into the cell wall carbohydrate composition of runner hyphae and stromata.					
37039647	10	70	theme	runner	1749:1754	arg1	hyphae					1756:1761	runner hyphae	1749:1761	runner hyphae	1749:1761	These changes are anticipated to enable the subcuticular differentiation of runner hyphae and stromata by V. inaequalis, as well as to protect these structures from host defenses and recognition by the host immune system.					
37039647	7	71	theme	inaequalis	1005:1014	arg1	wall					1021:1024	the V. inaequalis cell wall	998:1024	the V. inaequalis cell wall	998:1024	This analysis revealed that the V. inaequalis cell wall is mostly composed of glucans (44%) and mannans (37%), whereas chitin represents a much smaller proportion (4%).					
37039647	6	72	theme	hyphae	922:927	arg1	analysis					890:897	a glycosidic linkage analysis	869:897	a glycosidic linkage analysis of sporulating tubular hyphae	869:927	To better understand these processes, we first performed a glycosidic linkage analysis of sporulating tubular hyphae from V. inaequalis developed in culture.					
37039647	16	73	theme	V.	2719:2720	arg1	inaequalis					2722:2731	V. inaequalis	2719:2731	V. inaequalis	2719:2731	These changes are anticipated to enable host colonization by V. inaequalis and provide a foundation for understanding subcuticular host colonization by other plant-pathogenic fungi.					
37039647	7	74	theme	cell	1016:1019	arg1	wall					1021:1024	the V. inaequalis cell wall	998:1024	the V. inaequalis cell wall	998:1024	This analysis revealed that the V. inaequalis cell wall is mostly composed of glucans (44%) and mannans (37%), whereas chitin represents a much smaller proportion (4%).					
37039647	17	75	theme	plant-pathogenic	2958:2973	arg1	fungi					2975:2979	subcuticular plant-pathogenic fungi	2945:2979	subcuticular plant-pathogenic fungi	2945:2979	Such an understanding is important, as it may inform the development of novel control strategies against subcuticular plant-pathogenic fungi.					
37039647	15	76	theme	subcuticular	2394:2405	arg1	colonization					2412:2423	subcuticular host colonization	2394:2423	subcuticular host colonization	2394:2423	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	14	77	theme	Venturia	2267:2274	arg1	inaequalis					2276:2285	Venturia inaequalis	2267:2285	Venturia inaequalis	2267:2285	This is typified by Venturia inaequalis, which causes scab, the most economically important disease of apples.					
37039647	0	78	theme	Structures	72:81	arg1	Differentiation					43:57	the Differentiation	39:57	the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis	39:127	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.					
37039647	11	79	theme	Plant-pathogenic	1906:1921	arg1	fungi					1923:1927	IMPORTANCE Plant-pathogenic fungi	1895:1927	IMPORTANCE Plant-pathogenic fungi	1895:1927	IMPORTANCE Plant-pathogenic fungi are a major threat to food security.					
37039647	11	79	theme	Plant-pathogenic	1906:1921	arg1	threat					1941:1946	a major threat	1933:1946	a major threat to food security	1933:1963	IMPORTANCE Plant-pathogenic fungi are a major threat to food security.					
37039647	9	80	theme	carbohydrates	1454:1466	arg1	biosynthesis					1426:1437	the biosynthesis	1422:1437	the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan,	1422:1500	These analyses revealed that, during subcuticular host colonization, genes of V. inaequalis putatively associated with the biosynthesis of immunogenic carbohydrates, such as chitin and β-1,6-glucan, are downregulated relative to growth in culture, while on the surface of runner hyphae and stromata, chitin is deacetylated to the less-immunogenic carbohydrate chitosan.					
37039647	15	81	theme	less-immunogenic	2617:2632	arg1	carbohydrate					2634:2645	a less-immunogenic carbohydrate	2615:2645	a less-immunogenic carbohydrate	2615:2645	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	15	81	theme	less-immunogenic	2617:2632	arg1	chitosan					2648:2655	chitosan	2648:2655	chitosan	2648:2655	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	8	82	theme	wall	1242:1245	arg1	composition					1260:1270	the cell wall carbohydrate composition	1233:1270	the cell wall carbohydrate composition of runner hyphae and stromata	1233:1300	Next, we used transcriptomics and confocal laser scanning microscopy to provide insights into the cell wall carbohydrate composition of runner hyphae and stromata.					
37039647	0	83	theme	Scab	96:99	arg1	Fungus					101:106	the Apple Scab Fungus	86:106	the Apple Scab Fungus	86:106	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.					
37039647	0	83	theme	Scab	96:99	arg1	inaequalis					118:127	Venturia inaequalis	109:127	Venturia inaequalis	109:127	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.					
37039647	15	84	theme	infection	2589:2597	arg1	structures					2599:2608	its subcuticular infection structures	2572:2608	its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan	2572:2655	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	8	85	theme	confocal	1173:1180	arg1	microscopy					1197:1206	confocal laser scanning microscopy	1173:1206	confocal laser scanning microscopy	1173:1206	Next, we used transcriptomics and confocal laser scanning microscopy to provide insights into the cell wall carbohydrate composition of runner hyphae and stromata.					
37039647	8	86	theme	runner	1275:1280	arg1	hyphae					1282:1287	runner hyphae	1275:1287	runner hyphae	1275:1287	Next, we used transcriptomics and confocal laser scanning microscopy to provide insights into the cell wall carbohydrate composition of runner hyphae and stromata.					
37039647	0	87	theme	Wall	5:8	arg1	Dynamics					23:30	Cell Wall Carbohydrate Dynamics	0:30	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.	0:128	Cell Wall Carbohydrate Dynamics during the Differentiation of Infection Structures by the Apple Scab Fungus, Venturia inaequalis.					
37039647	5	88	theme	immune	797:802	arg1	system					804:809	the host immune system	788:809	the host immune system	788:809	Likewise, nothing is known about how these structures are protected from host defenses or recognition by the host immune system.					
37039647	13	89	theme	subcuticular	2169:2180	arg1	structures					2192:2201	specialized subcuticular infection structures	2157:2201	specialized subcuticular infection structures that, to date, remain largely understudied	2157:2244	A key feature of these pathogens is their ability to differentiate specialized subcuticular infection structures that, to date, remain largely understudied.					
37039647	15	90	theme	V.	2426:2427	arg1	inaequalis					2429:2438	V. inaequalis	2426:2438	V. inaequalis	2426:2438	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	16	91	theme	host	2698:2701	arg1	colonization					2703:2714	host colonization	2698:2714	host colonization by V. inaequalis	2698:2731	These changes are anticipated to enable host colonization by V. inaequalis and provide a foundation for understanding subcuticular host colonization by other plant-pathogenic fungi.					
37039647	1	92	theme	important	223:231	arg1	disease					233:239	the most economically important disease	201:239	the most economically important disease of apples	201:249	Scab, caused by the biotrophic fungal pathogen Venturia inaequalis, is the most economically important disease of apples.					
37039647	1	92	theme	important	223:231	arg1	Scab					130:133	Scab	130:133	Scab	130:133	Scab, caused by the biotrophic fungal pathogen Venturia inaequalis, is the most economically important disease of apples.					
37039647	17	93	theme	novel	2912:2916	arg1	strategies					2926:2935	novel control strategies	2912:2935	novel control strategies against subcuticular plant-pathogenic fungi	2912:2979	Such an understanding is important, as it may inform the development of novel control strategies against subcuticular plant-pathogenic fungi.					
37039647	3	94	theme	virulence	497:505	arg1	delivery					515:522	effector (virulence factor) delivery	487:522	effector (virulence factor) delivery	487:522	These structures are thought to be involved in nutrient acquisition and effector (virulence factor) delivery, but also give rise to conidia that further the infection cycle.					
37039647	15	95	theme	carbohydrates	2522:2534	arg1	biosynthesis					2480:2491	the biosynthesis	2476:2491	the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan,	2476:2560	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	11	96	theme	IMPORTANCE	1895:1904	arg1	fungi					1923:1927	IMPORTANCE Plant-pathogenic fungi	1895:1927	IMPORTANCE Plant-pathogenic fungi	1895:1927	IMPORTANCE Plant-pathogenic fungi are a major threat to food security.					
37039647	11	96	theme	IMPORTANCE	1895:1904	arg1	threat					1941:1946	a major threat	1933:1946	a major threat to food security	1933:1963	IMPORTANCE Plant-pathogenic fungi are a major threat to food security.					
37039647	15	97	theme	host	2407:2410	arg1	colonization					2412:2423	subcuticular host colonization	2394:2423	subcuticular host colonization	2394:2423	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	3	98	theme	effector	487:494	arg1	delivery					515:522	effector (virulence factor) delivery	487:522	effector (virulence factor) delivery	487:522	These structures are thought to be involved in nutrient acquisition and effector (virulence factor) delivery, but also give rise to conidia that further the infection cycle.					
37039647	15	99	theme	cell	2512:2515	arg1	β-1,6-glucan					2548:2559	β-1,6-glucan	2548:2559	β-1,6-glucan	2548:2559	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	15	99	theme	cell	2512:2515	arg1	chitin					2537:2542	chitin	2537:2542	chitin	2537:2542	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	15	99	theme	cell	2512:2515	arg1	carbohydrates					2522:2534	two immunogenic cell wall carbohydrates	2496:2534	two immunogenic cell wall carbohydrates	2496:2534	In this study, we show that, during subcuticular host colonization, V. inaequalis downregulates genes associated with the biosynthesis of two immunogenic cell wall carbohydrates, chitin and β-1,6-glucan, and coats its subcuticular infection structures with a less-immunogenic carbohydrate, chitosan.					
37039647	13	100	theme	key	2092:2094	arg1	feature					2096:2102	A key feature	2090:2102	A key feature of these pathogens	2090:2121	A key feature of these pathogens is their ability to differentiate specialized subcuticular infection structures that, to date, remain largely understudied.					
37039647	13	100	theme	key	2092:2094	arg1	ability					2132:2138	their ability to differentiate specialized subcuticular infection structures that, to date, remain largely understudied	2126:2244	their ability to differentiate specialized subcuticular infection structures that, to date, remain largely understudied	2126:2244	A key feature of these pathogens is their ability to differentiate specialized subcuticular infection structures that, to date, remain largely understudied.					
37039647	2	101	theme	infection	359:367	arg1	structures					369:378	specialized infection structures	347:378	specialized infection structures called runner hyphae and stromata	347:412	During infection, V. inaequalis colonizes the subcuticular host environment, where it develops specialized infection structures called runner hyphae and stromata.					
37092658	9	0	theme	PRACTICAL	1438:1446	arg1	APPLICATION					1448:1458	PRACTICAL APPLICATION	1438:1458	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.	1438:1620	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.					
37092658	1	1	theme	Vigna	219:223	arg1	GBF					245:247	GBF	245:247	GBF	245:247	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	1	theme	Vigna	219:223	arg1	flour					238:242	germinated Bambara groundnut (Vigna subterranea) flour	189:242	germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%)	189:307	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	4	2	theme	resistant	885:893	arg1	starch					895:900	resistant starch	885:900	resistant starch	885:900	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	2	theme	resistant	885:893	arg1	fiber					816:820	significantly higher dietary fiber	787:820	significantly higher dietary fiber	787:820	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	10	3	from	knowledge	1686:1694	arg1	characteristics					1718:1732	the dough handling characteristics	1699:1732	the dough handling characteristics	1699:1732	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	4	4	theme	total	946:950	arg1	flavonoids					952:961	total flavonoids	946:961	total flavonoids	946:961	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	4	theme	total	946:950	arg1	fiber					816:820	significantly higher dietary fiber	787:820	significantly higher dietary fiber	787:820	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	1	5	theme	Wheat	151:155	arg1	flour					157:161	Wheat flour	151:161	Wheat flour (WF)	151:166	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	5	theme	Wheat	151:155	arg1	WF					164:165	WF	164:165	WF	164:165	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	7	6	theme	20	1265:1266	arg1	%					1267:1267	%	1267:1267	%	1267:1267	Wheat bread supplemented with 20% GBF had significantly higher scores in taste, aroma, and overall acceptability.					
37092658	2	7	theme	nutritional	448:458	arg1	properties					393:402	The dough mixing, pasting, and gelatinization properties	347:402	The dough mixing, pasting, and gelatinization properties of the blends	347:416	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	7	theme	nutritional	448:458	arg1	quality					460:466	the nutritional quality	444:466	the nutritional quality	444:466	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	5	8	dep	GBF	1088:1090	arg1	%					1086:1086	%	1086:1086	%	1086:1086	The addition of up to 15% GBF had no significant impact on the specific volume of wheat bread.					
37092658	9	9	theme	wheat	1531:1535	arg1	flour					1537:1541	wheat flour	1531:1541	wheat flour	1531:1541	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.					
37092658	2	10	theme	sensory	571:577	arg1	properties					393:402	The dough mixing, pasting, and gelatinization properties	347:402	The dough mixing, pasting, and gelatinization properties of the blends	347:416	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	10	theme	sensory	571:577	arg1	properties					579:588	sensory properties	571:588	sensory properties	571:588	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	5	11	dep	15	1084:1085	arg1	to					1081:1082	to	1081:1082	to	1081:1082	The addition of up to 15% GBF had no significant impact on the specific volume of wheat bread.					
37092658	9	12	theme	partial	1507:1513	arg1	substitution					1515:1526	partial substitution	1507:1526	partial substitution of wheat flour with germinated Bambara groundnut	1507:1575	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.					
37092658	0	13	from	ingredient	61:70	arg1	bread					81:85	wheat bread	75:85	wheat bread	75:85	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.					
37092658	10	14	theme	wheat	1801:1805	arg1	dough					1807:1811	wheat dough	1801:1811	wheat dough with germinated Bambara groundnut	1801:1845	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	4	15	theme	significant	1002:1012	arg1	reduction					1014:1022	significant reduction	1002:1022	significant reduction in rapidly digestible starch content	1002:1059	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	0	16	theme	sensory	122:128	arg1	properties					130:139	Physicochemical, nutritional, and sensory properties	88:139	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.	0:149	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.					
37092658	5	17	contain	had	1092:1094	arg2	impact					1111:1116	no significant impact	1096:1116	no significant impact	1096:1116	The addition of up to 15% GBF had no significant impact on the specific volume of wheat bread.					
37092658	5	17	contain	had	1092:1094	arg1	addition					1066:1073	The addition	1062:1073	The addition of up to 15% GBF	1062:1090	The addition of up to 15% GBF had no significant impact on the specific volume of wheat bread.					
37092658	9	18	theme	flour	1537:1541	arg1	substitution					1515:1526	partial substitution	1507:1526	partial substitution of wheat flour with germinated Bambara groundnut	1507:1575	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.					
37092658	2	19	theme	antioxidant	528:538	arg1	properties					393:402	The dough mixing, pasting, and gelatinization properties	347:402	The dough mixing, pasting, and gelatinization properties of the blends	347:416	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	19	theme	antioxidant	528:538	arg1	potential					540:548	antioxidant potential	528:548	antioxidant potential	528:548	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	10	20	theme	Bambara	1829:1835	arg1	groundnut					1837:1845	germinated Bambara groundnut	1818:1845	germinated Bambara groundnut	1818:1845	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	4	21	theme	dietary	808:814	arg1	flavonoids					952:961	total flavonoids	946:961	total flavonoids	946:961	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	21	theme	dietary	808:814	arg1	scores					877:882	corrected amino acid scores	856:882	corrected amino acid scores	856:882	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	21	theme	dietary	808:814	arg1	digestibility					841:853	protein digestibility	833:853	protein digestibility	833:853	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	21	theme	dietary	808:814	arg1	starch					895:900	resistant starch	885:900	resistant starch	885:900	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	21	theme	dietary	808:814	arg1	fiber					816:820	significantly higher dietary fiber	787:820	significantly higher dietary fiber	787:820	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	21	theme	dietary	808:814	arg1	phenolics					935:943	total phenolics	929:943	total phenolics	929:943	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	21	theme	dietary	808:814	arg1	activities					980:989	antioxidant activities	968:989	antioxidant activities	968:989	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	21	theme	dietary	808:814	arg1	minerals					823:830	minerals	823:830	minerals	823:830	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	21	theme	dietary	808:814	arg1	starch					921:926	slowly digestible starch	903:926	slowly digestible starch	903:926	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	22	theme	antioxidant	968:978	arg1	fiber					816:820	significantly higher dietary fiber	787:820	significantly higher dietary fiber	787:820	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	22	theme	antioxidant	968:978	arg1	activities					980:989	antioxidant activities	968:989	antioxidant activities	968:989	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	6	23	theme	color	1196:1200	arg1	properties					1214:1223	color and texture properties	1196:1223	color and texture properties of bread	1196:1232	Substitution of WF with GBF influenced color and texture properties of bread.					
37092658	9	24	dep	APPLICATION	1448:1458	arg1	provides					1472:1479	provides	1472:1479	provides	1472:1479	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.					
37092658	7	25	contain	had	1273:1275	arg2	scores					1298:1303	significantly higher scores	1277:1303	significantly higher scores	1277:1303	Wheat bread supplemented with 20% GBF had significantly higher scores in taste, aroma, and overall acceptability.					
37092658	7	25	contain	had	1273:1275	arg1	bread					1241:1245	Wheat bread	1235:1245	Wheat bread supplemented with 20% GBF	1235:1271	Wheat bread supplemented with 20% GBF had significantly higher scores in taste, aroma, and overall acceptability.					
37092658	3	26	theme	absorption	653:662	arg1	capacity					664:671	higher water absorption capacity	640:671	higher water absorption capacity	640:671	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	3	27	theme	dough	703:707	arg1	time					721:724	dough development time	703:724	dough development time	703:724	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	2	28	theme	pasting	365:371	arg1	texture					558:564	texture	558:564	texture	558:564	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	28	theme	pasting	365:371	arg1	digestibility					485:497	in vitro starch digestibility	469:497	in vitro starch digestibility	469:497	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	28	theme	pasting	365:371	arg1	potential					540:548	antioxidant potential	528:548	antioxidant potential	528:548	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	28	theme	pasting	365:371	arg1	color					551:555	color	551:555	color	551:555	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	28	theme	pasting	365:371	arg1	quality					460:466	the nutritional quality	444:466	the nutritional quality	444:466	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	28	theme	pasting	365:371	arg1	properties					579:588	sensory properties	571:588	sensory properties	571:588	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	28	theme	pasting	365:371	arg1	properties					393:402	The dough mixing, pasting, and gelatinization properties	347:402	The dough mixing, pasting, and gelatinization properties of the blends	347:416	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	28	theme	pasting	365:371	arg1	constituents					514:525	phytochemical constituents	500:525	phytochemical constituents	500:525	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	29	theme	starch	478:483	arg1	properties					393:402	The dough mixing, pasting, and gelatinization properties	347:402	The dough mixing, pasting, and gelatinization properties of the blends	347:416	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	29	theme	starch	478:483	arg1	digestibility					485:497	in vitro starch digestibility	469:497	in vitro starch digestibility	469:497	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	5	30	theme	GBF	1088:1090	arg1	addition					1066:1073	The addition	1062:1073	The addition of up to 15% GBF	1062:1090	The addition of up to 15% GBF had no significant impact on the specific volume of wheat bread.					
37092658	3	31	theme	higher	640:645	arg1	capacity					664:671	higher water absorption capacity	640:671	higher water absorption capacity	640:671	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	0	32	theme	wheat	75:79	arg1	bread					81:85	wheat bread	75:85	wheat bread	75:85	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.					
37092658	8	33	from	ingredient	1410:1419	arg1	bread					1424:1428	bread making	1424:1435	bread making	1424:1435	This study demonstrated the potential of GBF as a functional ingredient in bread making.					
37092658	2	34	theme	mixing	357:362	arg1	texture					558:564	texture	558:564	texture	558:564	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	34	theme	mixing	357:362	arg1	digestibility					485:497	in vitro starch digestibility	469:497	in vitro starch digestibility	469:497	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	34	theme	mixing	357:362	arg1	potential					540:548	antioxidant potential	528:548	antioxidant potential	528:548	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	34	theme	mixing	357:362	arg1	color					551:555	color	551:555	color	551:555	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	34	theme	mixing	357:362	arg1	quality					460:466	the nutritional quality	444:466	the nutritional quality	444:466	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	34	theme	mixing	357:362	arg1	properties					579:588	sensory properties	571:588	sensory properties	571:588	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	34	theme	mixing	357:362	arg1	properties					393:402	The dough mixing, pasting, and gelatinization properties	347:402	The dough mixing, pasting, and gelatinization properties of the blends	347:416	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	34	theme	mixing	357:362	arg1	constituents					514:525	phytochemical constituents	500:525	phytochemical constituents	500:525	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	35	theme	in	469:470	arg1	properties					393:402	The dough mixing, pasting, and gelatinization properties	347:402	The dough mixing, pasting, and gelatinization properties of the blends	347:416	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	35	theme	in	469:470	arg1	digestibility					485:497	in vitro starch digestibility	469:497	in vitro starch digestibility	469:497	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	5	36	theme	wheat	1144:1148	arg1	bread					1150:1154	wheat bread	1144:1154	wheat bread	1144:1154	The addition of up to 15% GBF had no significant impact on the specific volume of wheat bread.					
37092658	0	37	theme	subterranea	36:46	arg1	flour					49:53	Germinated Bambara groundnut (Vigna subterranea) flour	0:53	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.	0:149	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.					
37092658	3	38	theme	low	727:729	arg1	peak					731:734	low peak	727:734	low peak	727:734	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	4	39	theme	corrected	856:864	arg1	scores					877:882	corrected amino acid scores	856:882	corrected amino acid scores	856:882	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	39	theme	corrected	856:864	arg1	fiber					816:820	significantly higher dietary fiber	787:820	significantly higher dietary fiber	787:820	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	40	theme	digestible	1035:1044	arg1	starch					1046:1051	rapidly digestible starch	1027:1051	rapidly digestible starch content	1027:1059	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	0	41	theme	Germinated	0:9	arg1	flour					49:53	Germinated Bambara groundnut (Vigna subterranea) flour	0:53	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.	0:149	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.					
37092658	9	42	theme	germinated	1548:1557	arg1	groundnut					1567:1575	germinated Bambara groundnut	1548:1575	germinated Bambara groundnut	1548:1575	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.					
37092658	4	43	theme	acid	872:875	arg1	scores					877:882	corrected amino acid scores	856:882	corrected amino acid scores	856:882	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	43	theme	acid	872:875	arg1	fiber					816:820	significantly higher dietary fiber	787:820	significantly higher dietary fiber	787:820	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	0	44	theme	groundnut	19:27	arg1	flour					49:53	Germinated Bambara groundnut (Vigna subterranea) flour	0:53	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.	0:149	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.					
37092658	7	45	theme	overall	1326:1332	arg1	acceptability					1334:1346	overall acceptability	1326:1346	overall acceptability	1326:1346	Wheat bread supplemented with 20% GBF had significantly higher scores in taste, aroma, and overall acceptability.					
37092658	3	46	theme	setback	741:747	arg1	viscosities					749:759	setback viscosities	741:759	setback viscosities	741:759	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	0	47	theme	Vigna	30:34	arg1	flour					49:53	Germinated Bambara groundnut (Vigna subterranea) flour	0:53	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.	0:149	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.					
37092658	4	48	contain	had	783:785	arg2	activities					980:989	antioxidant activities	968:989	antioxidant activities	968:989	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	48	contain	had	783:785	arg2	phenolics					935:943	total phenolics	929:943	total phenolics	929:943	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	48	contain	had	783:785	arg2	starch					921:926	slowly digestible starch	903:926	slowly digestible starch	903:926	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	48	contain	had	783:785	arg2	flavonoids					952:961	total flavonoids	946:961	total flavonoids	946:961	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	48	contain	had	783:785	arg2	minerals					823:830	minerals	823:830	minerals	823:830	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	48	contain	had	783:785	arg2	digestibility					841:853	protein digestibility	833:853	protein digestibility	833:853	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	48	contain	had	783:785	arg1	breads					776:781	The composite breads	762:781	The composite breads	762:781	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	48	contain	had	783:785	arg2	scores					877:882	corrected amino acid scores	856:882	corrected amino acid scores	856:882	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	48	contain	had	783:785	arg2	fiber					816:820	significantly higher dietary fiber	787:820	significantly higher dietary fiber	787:820	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	48	contain	had	783:785	arg2	starch					895:900	resistant starch	885:900	resistant starch	885:900	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	9	49	theme	Bambara	1559:1565	arg1	groundnut					1567:1575	germinated Bambara groundnut	1548:1575	germinated Bambara groundnut	1548:1575	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.					
37092658	1	50	theme	bread	340:344	arg1	preparation					325:335	the preparation	321:335	the preparation of bread	321:344	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	6	51	theme	texture	1206:1212	arg1	properties					1214:1223	color and texture properties	1196:1223	color and texture properties of bread	1196:1232	Substitution of WF with GBF influenced color and texture properties of bread.					
37092658	3	52	contain	containing	621:630	arg1	dough					615:619	All the wheat dough	601:619	All the wheat dough containing GBF	601:634	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	3	52	contain	containing	621:630	arg2	GBF					632:634	GBF	632:634	GBF	632:634	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	3	53	contain	had	636:638	arg2	capacity					664:671	higher water absorption capacity	640:671	higher water absorption capacity	640:671	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	3	53	contain	had	636:638	arg2	viscosities					749:759	setback viscosities	741:759	setback viscosities	741:759	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	3	53	contain	had	636:638	arg2	time					721:724	dough development time	703:724	dough development time	703:724	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	3	53	contain	had	636:638	arg1	dough					615:619	All the wheat dough	601:619	All the wheat dough containing GBF	601:634	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	3	53	contain	had	636:638	arg2	peak					731:734	low peak	727:734	low peak	727:734	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	3	53	contain	had	636:638	arg2	temperatures					689:700	gelatinization temperatures	674:700	gelatinization temperatures	674:700	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	1	54	used	used	313:316	arg2	WF					164:165	WF	164:165	WF	164:165	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	54	used	used	313:316	arg2	flour					157:161	Wheat flour	151:161	Wheat flour (WF)	151:166	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	55	theme	Bambara	200:206	arg1	GBF					245:247	GBF	245:247	GBF	245:247	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	55	theme	Bambara	200:206	arg1	flour					238:242	germinated Bambara groundnut (Vigna subterranea) flour	189:242	germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%)	189:307	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	10	56	from	results	1651:1657	arg1	study					1667:1671	this study	1662:1671	this study	1662:1671	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	7	57	theme	higher	1291:1296	arg1	scores					1298:1303	significantly higher scores	1277:1303	significantly higher scores	1277:1303	Wheat bread supplemented with 20% GBF had significantly higher scores in taste, aroma, and overall acceptability.					
37092658	10	58	theme	dough	1807:1811	arg1	properties					1787:1796	the mixing properties	1776:1796	the mixing properties of wheat dough with germinated Bambara groundnut	1776:1845	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	8	59	theme	functional	1399:1408	arg1	ingredient					1410:1419	a functional ingredient	1397:1419	a functional ingredient in bread making	1397:1435	This study demonstrated the potential of GBF as a functional ingredient in bread making.					
37092658	10	60	theme	dough	1703:1707	arg1	characteristics					1718:1732	the dough handling characteristics	1699:1732	the dough handling characteristics	1699:1732	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	1	61	from	proportions	263:273	arg1	GBF					245:247	GBF	245:247	GBF	245:247	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	61	from	proportions	263:273	arg1	flour					238:242	germinated Bambara groundnut (Vigna subterranea) flour	189:242	germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%)	189:307	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	62	theme	subterranea	225:235	arg1	GBF					245:247	GBF	245:247	GBF	245:247	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	62	theme	subterranea	225:235	arg1	flour					238:242	germinated Bambara groundnut (Vigna subterranea) flour	189:242	germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%)	189:307	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	7	63	theme	%	1267:1267	arg1	GBF					1269:1271	20% GBF	1265:1271	20% GBF	1265:1271	Wheat bread supplemented with 20% GBF had significantly higher scores in taste, aroma, and overall acceptability.					
37092658	10	64	with	dough	1807:1811	arg1	groundnut					1837:1845	germinated Bambara groundnut	1818:1845	germinated Bambara groundnut	1818:1845	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	2	65	theme	breads	593:598	arg1	texture					558:564	texture	558:564	texture	558:564	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	65	theme	breads	593:598	arg1	digestibility					485:497	in vitro starch digestibility	469:497	in vitro starch digestibility	469:497	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	65	theme	breads	593:598	arg1	potential					540:548	antioxidant potential	528:548	antioxidant potential	528:548	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	65	theme	breads	593:598	arg1	color					551:555	color	551:555	color	551:555	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	65	theme	breads	593:598	arg1	quality					460:466	the nutritional quality	444:466	the nutritional quality	444:466	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	65	theme	breads	593:598	arg1	properties					579:588	sensory properties	571:588	sensory properties	571:588	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	65	theme	breads	593:598	arg1	properties					393:402	The dough mixing, pasting, and gelatinization properties	347:402	The dough mixing, pasting, and gelatinization properties of the blends	347:416	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	65	theme	breads	593:598	arg1	constituents					514:525	phytochemical constituents	500:525	phytochemical constituents	500:525	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	8	66	theme	making	1430:1435	arg1	bread					1424:1428	bread making	1424:1435	bread making	1424:1435	This study demonstrated the potential of GBF as a functional ingredient in bread making.					
37092658	5	67	dep	%	1086:1086	arg1	15					1084:1085	15	1084:1085	15	1084:1085	The addition of up to 15% GBF had no significant impact on the specific volume of wheat bread.					
37092658	7	68	theme	Wheat	1235:1239	arg1	bread					1241:1245	Wheat bread	1235:1245	Wheat bread supplemented with 20% GBF	1235:1271	Wheat bread supplemented with 20% GBF had significantly higher scores in taste, aroma, and overall acceptability.					
37092658	9	69	theme	suitable	1483:1490	arg1	possibility					1492:1502	a suitable possibility	1481:1502	a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut	1481:1575	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.					
37092658	4	70	theme	total	929:933	arg1	phenolics					935:943	total phenolics	929:943	total phenolics	929:943	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	70	theme	total	929:933	arg1	fiber					816:820	significantly higher dietary fiber	787:820	significantly higher dietary fiber	787:820	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	0	71	theme	nutritional	105:115	arg1	properties					130:139	Physicochemical, nutritional, and sensory properties	88:139	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.	0:149	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.					
37092658	2	72	theme	gelatinization	378:391	arg1	texture					558:564	texture	558:564	texture	558:564	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	72	theme	gelatinization	378:391	arg1	digestibility					485:497	in vitro starch digestibility	469:497	in vitro starch digestibility	469:497	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	72	theme	gelatinization	378:391	arg1	potential					540:548	antioxidant potential	528:548	antioxidant potential	528:548	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	72	theme	gelatinization	378:391	arg1	color					551:555	color	551:555	color	551:555	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	72	theme	gelatinization	378:391	arg1	quality					460:466	the nutritional quality	444:466	the nutritional quality	444:466	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	72	theme	gelatinization	378:391	arg1	properties					579:588	sensory properties	571:588	sensory properties	571:588	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	72	theme	gelatinization	378:391	arg1	properties					393:402	The dough mixing, pasting, and gelatinization properties	347:402	The dough mixing, pasting, and gelatinization properties of the blends	347:416	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	72	theme	gelatinization	378:391	arg1	constituents					514:525	phytochemical constituents	500:525	phytochemical constituents	500:525	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	10	73	theme	limited	1746:1752	arg1	information					1754:1764	limited information	1746:1764	limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut	1746:1845	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	9	74	theme	substitution	1515:1526	arg1	possibility					1492:1502	a suitable possibility	1481:1502	a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut	1481:1575	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.					
37092658	3	75	theme	wheat	609:613	arg1	dough					615:619	All the wheat dough	601:619	All the wheat dough containing GBF	601:634	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	2	76	theme	blends	411:416	arg1	texture					558:564	texture	558:564	texture	558:564	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	76	theme	blends	411:416	arg1	digestibility					485:497	in vitro starch digestibility	469:497	in vitro starch digestibility	469:497	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	76	theme	blends	411:416	arg1	potential					540:548	antioxidant potential	528:548	antioxidant potential	528:548	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	76	theme	blends	411:416	arg1	color					551:555	color	551:555	color	551:555	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	76	theme	blends	411:416	arg1	quality					460:466	the nutritional quality	444:466	the nutritional quality	444:466	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	76	theme	blends	411:416	arg1	properties					579:588	sensory properties	571:588	sensory properties	571:588	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	76	theme	blends	411:416	arg1	properties					393:402	The dough mixing, pasting, and gelatinization properties	347:402	The dough mixing, pasting, and gelatinization properties of the blends	347:416	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	76	theme	blends	411:416	arg1	constituents					514:525	phytochemical constituents	500:525	phytochemical constituents	500:525	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	10	77	theme	mixing	1780:1785	arg1	properties					1787:1796	the mixing properties	1776:1796	the mixing properties of wheat dough with germinated Bambara groundnut	1776:1845	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	0	78	theme	bread	144:148	arg1	properties					130:139	Physicochemical, nutritional, and sensory properties	88:139	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.	0:149	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.					
37092658	10	79	theme	pasting	1643:1649	arg1	results					1651:1657	The dough mixing and pasting results	1622:1657	The dough mixing and pasting results in this study	1622:1671	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	5	80	theme	significant	1099:1109	arg1	impact					1111:1116	no significant impact	1096:1116	no significant impact	1096:1116	The addition of up to 15% GBF had no significant impact on the specific volume of wheat bread.					
37092658	10	81	theme	germinated	1818:1827	arg1	groundnut					1837:1845	germinated Bambara groundnut	1818:1845	germinated Bambara groundnut	1818:1845	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	10	82	theme	mixing	1632:1637	arg1	results					1651:1657	The dough mixing and pasting results	1622:1657	The dough mixing and pasting results in this study	1622:1671	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	6	83	with	Substitution	1157:1168	arg1	GBF					1181:1183	GBF	1181:1183	GBF	1181:1183	Substitution of WF with GBF influenced color and texture properties of bread.					
37092658	3	84	theme	gelatinization	674:687	arg1	temperatures					689:700	gelatinization temperatures	674:700	gelatinization temperatures	674:700	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	4	85	theme	higher	801:806	arg1	flavonoids					952:961	total flavonoids	946:961	total flavonoids	946:961	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	85	theme	higher	801:806	arg1	scores					877:882	corrected amino acid scores	856:882	corrected amino acid scores	856:882	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	85	theme	higher	801:806	arg1	digestibility					841:853	protein digestibility	833:853	protein digestibility	833:853	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	85	theme	higher	801:806	arg1	starch					895:900	resistant starch	885:900	resistant starch	885:900	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	85	theme	higher	801:806	arg1	fiber					816:820	significantly higher dietary fiber	787:820	significantly higher dietary fiber	787:820	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	85	theme	higher	801:806	arg1	phenolics					935:943	total phenolics	929:943	total phenolics	929:943	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	85	theme	higher	801:806	arg1	activities					980:989	antioxidant activities	968:989	antioxidant activities	968:989	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	85	theme	higher	801:806	arg1	minerals					823:830	minerals	823:830	minerals	823:830	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	85	theme	higher	801:806	arg1	starch					921:926	slowly digestible starch	903:926	slowly digestible starch	903:926	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	6	86	theme	WF	1173:1174	arg1	Substitution					1157:1168	Substitution	1157:1168	Substitution of WF with GBF	1157:1183	Substitution of WF with GBF influenced color and texture properties of bread.					
37092658	2	87	dep	in	469:470	arg1	vitro					472:476	vitro	472:476	vitro	472:476	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	3	88	theme	development	709:719	arg1	time					721:724	dough development time	703:724	dough development time	703:724	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	4	89	theme	composite	766:774	arg1	breads					776:781	The composite breads	762:781	The composite breads	762:781	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	1	90	dep	proportions	263:273	arg1	%					292:292	20%	290:292	20%	290:292	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	90	dep	proportions	263:273	arg1	%					306:306	30%	304:306	30%	304:306	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	90	dep	proportions	263:273	arg1	%					282:282	10%	280:282	10%	280:282	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	90	dep	proportions	263:273	arg1	%					287:287	15%	285:287	15%	285:287	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	90	dep	proportions	263:273	arg1	%					297:297	25%	295:297	25%	295:297	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	90	dep	proportions	263:273	arg1	%					277:277	5%	276:277	5%	276:277	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	3	91	theme	water	647:651	arg1	capacity					664:671	higher water absorption capacity	640:671	higher water absorption capacity	640:671	All the wheat dough containing GBF had higher water absorption capacity, gelatinization temperatures, dough development time, low peak, and setback viscosities.					
37092658	0	92	theme	Physicochemical	88:102	arg1	properties					130:139	Physicochemical, nutritional, and sensory properties	88:139	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.	0:149	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.					
37092658	2	93	theme	dough	351:355	arg1	mixing					357:362	The dough mixing	347:362	The dough mixing	347:362	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	4	94	theme	starch	1046:1051	arg1	content					1053:1059	rapidly digestible starch content	1027:1059	rapidly digestible starch content	1027:1059	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	5	95	theme	bread	1150:1154	arg1	volume					1134:1139	the specific volume	1121:1139	the specific volume of wheat bread	1121:1154	The addition of up to 15% GBF had no significant impact on the specific volume of wheat bread.					
37092658	9	96	theme	functional	1589:1598	arg1	bread					1615:1619	functional and acceptable bread	1589:1619	functional and acceptable bread	1589:1619	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.					
37092658	0	97	theme	Bambara	11:17	arg1	flour					49:53	Germinated Bambara groundnut (Vigna subterranea) flour	0:53	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.	0:149	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.					
37092658	2	98	theme	phytochemical	500:512	arg1	properties					393:402	The dough mixing, pasting, and gelatinization properties	347:402	The dough mixing, pasting, and gelatinization properties of the blends	347:416	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	2	98	theme	phytochemical	500:512	arg1	constituents					514:525	phytochemical constituents	500:525	phytochemical constituents	500:525	The dough mixing, pasting, and gelatinization properties of the blends were evaluated as well as the nutritional quality, in vitro starch digestibility, phytochemical constituents, antioxidant potential, color, texture, and sensory properties of breads.					
37092658	4	99	theme	amino	866:870	arg1	scores					877:882	corrected amino acid scores	856:882	corrected amino acid scores	856:882	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	99	theme	amino	866:870	arg1	fiber					816:820	significantly higher dietary fiber	787:820	significantly higher dietary fiber	787:820	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	5	100	theme	specific	1125:1132	arg1	volume					1134:1139	the specific volume	1121:1139	the specific volume of wheat bread	1121:1154	The addition of up to 15% GBF had no significant impact on the specific volume of wheat bread.					
37092658	9	101	with	substitution	1515:1526	arg1	groundnut					1567:1575	germinated Bambara groundnut	1548:1575	germinated Bambara groundnut	1548:1575	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.					
37092658	6	102	theme	bread	1228:1232	arg1	properties					1214:1223	color and texture properties	1196:1223	color and texture properties of bread	1196:1232	Substitution of WF with GBF influenced color and texture properties of bread.					
37092658	0	103	dep	flour	49:53	arg1	properties					130:139	Physicochemical, nutritional, and sensory properties	88:139	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.	0:149	Germinated Bambara groundnut (Vigna subterranea) flour as an ingredient in wheat bread: Physicochemical, nutritional, and sensory properties of bread.					
37092658	1	104	theme	different	253:261	arg1	proportions					263:273	different proportions	253:273	different proportions (5%, 10%, 15%, 20%, 25%, and 30%)	253:307	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	4	105	from	reduction	1014:1022	arg1	content					1053:1059	rapidly digestible starch content	1027:1059	rapidly digestible starch content	1027:1059	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	106	theme	protein	833:839	arg1	digestibility					841:853	protein digestibility	833:853	protein digestibility	833:853	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	106	theme	protein	833:839	arg1	fiber					816:820	significantly higher dietary fiber	787:820	significantly higher dietary fiber	787:820	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	8	107	theme	GBF	1390:1392	arg1	potential					1377:1385	the potential	1373:1385	the potential of GBF as a functional ingredient in bread making	1373:1435	This study demonstrated the potential of GBF as a functional ingredient in bread making.					
37092658	1	108	theme	germinated	189:198	arg1	GBF					245:247	GBF	245:247	GBF	245:247	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	108	theme	germinated	189:198	arg1	flour					238:242	germinated Bambara groundnut (Vigna subterranea) flour	189:242	germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%)	189:307	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	4	109	theme	digestible	910:919	arg1	fiber					816:820	significantly higher dietary fiber	787:820	significantly higher dietary fiber	787:820	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	4	109	theme	digestible	910:919	arg1	starch					921:926	slowly digestible starch	903:926	slowly digestible starch	903:926	The composite breads had significantly higher dietary fiber, minerals, protein digestibility, corrected amino acid scores, resistant starch, slowly digestible starch, total phenolics, total flavonoids, and antioxidant activities and caused significant reduction in rapidly digestible starch content.					
37092658	10	110	theme	handling	1709:1716	arg1	characteristics					1718:1732	the dough handling characteristics	1699:1732	the dough handling characteristics	1699:1732	The dough mixing and pasting results in this study would add to knowledge on the dough handling characteristics as there is limited information regarding the mixing properties of wheat dough with germinated Bambara groundnut.					
37092658	9	111	theme	acceptable	1604:1613	arg1	bread					1615:1619	functional and acceptable bread	1589:1619	functional and acceptable bread	1589:1619	PRACTICAL APPLICATION: This study provides a suitable possibility of partial substitution of wheat flour with germinated Bambara groundnut, to develop functional and acceptable bread.					
37092658	1	112	theme	groundnut	208:216	arg1	GBF					245:247	GBF	245:247	GBF	245:247	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
37092658	1	112	theme	groundnut	208:216	arg1	flour					238:242	germinated Bambara groundnut (Vigna subterranea) flour	189:242	germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%)	189:307	Wheat flour (WF) was substituted with germinated Bambara groundnut (Vigna subterranea) flour (GBF) at different proportions (5%, 10%, 15%, 20%, 25%, and 30%) and used in the preparation of bread.					
35710072	7	0	theme	ST.	1125:1127	arg1	efficiency					1088:1097	high efficiency	1083:1097	high efficiency	1083:1097	The results indicated that ST-GMS/ST-HA MNs, in comparison with the traditional subcutaneous application approach, achieved both high efficiency and continuous release of ST. Therefore, this device is promising for the delivery ST for allergic asthma therapy.					
35710072	7	0	theme	ST.	1125:1127	arg1	release					1114:1120	continuous release	1103:1120	continuous release of ST.	1103:1127	The results indicated that ST-GMS/ST-HA MNs, in comparison with the traditional subcutaneous application approach, achieved both high efficiency and continuous release of ST. Therefore, this device is promising for the delivery ST for allergic asthma therapy.					
35710072	7	1	theme	subcutaneous	1034:1045	arg1	approach					1059:1066	the traditional subcutaneous application approach	1018:1066	the traditional subcutaneous application approach	1018:1066	The results indicated that ST-GMS/ST-HA MNs, in comparison with the traditional subcutaneous application approach, achieved both high efficiency and continuous release of ST. Therefore, this device is promising for the delivery ST for allergic asthma therapy.					
35710072	3	2	theme	two-step	453:460	arg1	method					487:492	a two-step centrifuging and molding method	451:492	a two-step centrifuging and molding method	451:492	To immobilize ST in MNs, we used a two-step centrifuging and molding method.					
35710072	0	3	theme	allergic	106:113	arg1	asthma					115:120	allergic asthma	106:120	allergic asthma in guinea pigs	106:135	Transdermal delivery of sinapine thiocyanate by gelatin microspheres and hyaluronic acid microneedles for allergic asthma in guinea pigs.					
35710072	5	4	from	site	758:761	arg1	penetration					728:738	progressive penetration	716:738	progressive penetration from the puncture site into deeper tissues	716:781	In vitro, skin implantation ability was assessed with fluorescein isothiocyanate staining, which revealed progressive penetration from the puncture site into deeper tissues.					
35710072	3	5	theme	centrifuging	462:473	arg1	method					487:492	a two-step centrifuging and molding method	451:492	a two-step centrifuging and molding method	451:492	To immobilize ST in MNs, we used a two-step centrifuging and molding method.					
35710072	6	6	dep	in	903:904	arg1	vivo					906:909	vivo	906:909	vivo	906:909	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	7	7	theme	asthma	1198:1203	arg1	therapy					1205:1211	allergic asthma therapy	1189:1211	allergic asthma therapy	1189:1211	The results indicated that ST-GMS/ST-HA MNs, in comparison with the traditional subcutaneous application approach, achieved both high efficiency and continuous release of ST. Therefore, this device is promising for the delivery ST for allergic asthma therapy.					
35710072	1	8	theme	permeable	261:269	arg1	medicines					271:279	poorly permeable medicines	254:279	poorly permeable medicines	254:279	Dissolving microneedles (MNs) are an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines.					
35710072	7	9	theme	ST	1182:1183	arg1	delivery					1173:1180	the delivery	1169:1180	the delivery ST for allergic asthma therapy	1169:1211	The results indicated that ST-GMS/ST-HA MNs, in comparison with the traditional subcutaneous application approach, achieved both high efficiency and continuous release of ST. Therefore, this device is promising for the delivery ST for allergic asthma therapy.					
35710072	3	10	theme	molding	479:485	arg1	method					487:492	a two-step centrifuging and molding method	451:492	a two-step centrifuging and molding method	451:492	To immobilize ST in MNs, we used a two-step centrifuging and molding method.					
35710072	6	11	from	feasibility	788:798	arg1	pigs					870:873	allergic asthma guinea pigs	847:873	allergic asthma guinea pigs	847:873	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	5	12	theme	deeper	768:773	arg1	tissues					775:781	deeper tissues	768:781	deeper tissues	768:781	In vitro, skin implantation ability was assessed with fluorescein isothiocyanate staining, which revealed progressive penetration from the puncture site into deeper tissues.					
35710072	6	13	theme	guinea	863:868	arg1	pigs					870:873	allergic asthma guinea pigs	847:873	allergic asthma guinea pigs	847:873	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	7	14	theme	continuous	1103:1112	arg1	release					1114:1120	continuous release	1103:1120	continuous release of ST.	1103:1127	The results indicated that ST-GMS/ST-HA MNs, in comparison with the traditional subcutaneous application approach, achieved both high efficiency and continuous release of ST. Therefore, this device is promising for the delivery ST for allergic asthma therapy.					
35710072	6	15	theme	asthma	856:861	arg1	pigs					870:873	allergic asthma guinea pigs	847:873	allergic asthma guinea pigs	847:873	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	7	16	theme	high	1083:1086	arg1	efficiency					1088:1097	high efficiency	1083:1097	high efficiency	1083:1097	The results indicated that ST-GMS/ST-HA MNs, in comparison with the traditional subcutaneous application approach, achieved both high efficiency and continuous release of ST. Therefore, this device is promising for the delivery ST for allergic asthma therapy.					
35710072	5	17	theme	progressive	716:726	arg1	penetration					728:738	progressive penetration	716:738	progressive penetration from the puncture site into deeper tissues	716:781	In vitro, skin implantation ability was assessed with fluorescein isothiocyanate staining, which revealed progressive penetration from the puncture site into deeper tissues.					
35710072	7	18	theme	application	1047:1057	arg1	approach					1059:1066	the traditional subcutaneous application approach	1018:1066	the traditional subcutaneous application approach	1018:1066	The results indicated that ST-GMS/ST-HA MNs, in comparison with the traditional subcutaneous application approach, achieved both high efficiency and continuous release of ST. Therefore, this device is promising for the delivery ST for allergic asthma therapy.					
35710072	1	19	theme	efficient	175:183	arg1	method					215:220	an efficient, safe, and generally painless method	172:220	an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines	172:279	Dissolving microneedles (MNs) are an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines.					
35710072	1	19	theme	efficient	175:183	arg1	microneedles					149:160	Dissolving microneedles	138:160	Dissolving microneedles (MNs)	138:166	Dissolving microneedles (MNs) are an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines.					
35710072	0	20	theme	Transdermal	0:10	arg1	delivery					12:19	Transdermal delivery	0:19	Transdermal delivery of sinapine thiocyanate by gelatin microspheres and hyaluronic acid	0:87	Transdermal delivery of sinapine thiocyanate by gelatin microspheres and hyaluronic acid microneedles for allergic asthma in guinea pigs.					
35710072	1	21	theme	medicines	271:279	arg1	distribution					238:249	transdermal distribution	226:249	transdermal distribution of poorly permeable medicines	226:279	Dissolving microneedles (MNs) are an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines.					
35710072	2	22	theme	gelatin	365:371	arg1	GMS					387:389	GMS	387:389	GMS	387:389	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	2	22	theme	gelatin	365:371	arg1	microspheres					373:384	sinapine thiocyanate (ST)-loaded gelatin microspheres	332:384	sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS)	332:390	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	0	23	theme	sinapine	24:31	arg1	thiocyanate					33:43	sinapine thiocyanate	24:43	sinapine thiocyanate	24:43	Transdermal delivery of sinapine thiocyanate by gelatin microspheres and hyaluronic acid microneedles for allergic asthma in guinea pigs.					
35710072	4	24	theme	ST-GMS/ST-HA	500:511	arg1	MNs					513:515	ST-GMS/ST-HA MNs	500:515	ST-GMS/ST-HA MNs	500:515	When ST-GMS/ST-HA MNs were placed on the skin, they showed extraordinary mechanical strength and dissolved slowly.					
35710072	2	25	theme	composite	299:307	arg1	MNs					309:311	dissolving composite MNs	288:311	dissolving composite MNs	288:311	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	1	26	theme	safe	186:189	arg1	method					215:220	an efficient, safe, and generally painless method	172:220	an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines	172:279	Dissolving microneedles (MNs) are an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines.					
35710072	1	26	theme	safe	186:189	arg1	microneedles					149:160	Dissolving microneedles	138:160	Dissolving microneedles (MNs)	138:166	Dissolving microneedles (MNs) are an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines.					
35710072	0	27	theme	guinea	125:130	arg1	pigs					132:135	guinea pigs	125:135	guinea pigs	125:135	Transdermal delivery of sinapine thiocyanate by gelatin microspheres and hyaluronic acid microneedles for allergic asthma in guinea pigs.					
35710072	6	28	theme	allergic	847:854	arg1	asthma					856:861	allergic asthma	847:861	allergic asthma guinea pigs	847:873	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	2	29	theme	dissolving	288:297	arg1	MNs					309:311	dissolving composite MNs	288:311	dissolving composite MNs	288:311	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	2	30	theme	hyaluronic	396:405	arg1	HA					413:414	HA	413:414	HA	413:414	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	2	30	theme	hyaluronic	396:405	arg1	acid					407:410	hyaluronic acid	396:410	hyaluronic acid (HA)	396:415	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	5	31	theme	puncture	749:756	arg1	site					758:761	the puncture site	745:761	the puncture site	745:761	In vitro, skin implantation ability was assessed with fluorescein isothiocyanate staining, which revealed progressive penetration from the puncture site into deeper tissues.					
35710072	5	32	theme	fluorescein	664:674	arg1	staining					691:698	fluorescein isothiocyanate staining	664:698	fluorescein isothiocyanate staining	664:698	In vitro, skin implantation ability was assessed with fluorescein isothiocyanate staining, which revealed progressive penetration from the puncture site into deeper tissues.					
35710072	0	33	theme	thiocyanate	33:43	arg1	delivery					12:19	Transdermal delivery	0:19	Transdermal delivery of sinapine thiocyanate by gelatin microspheres and hyaluronic acid	0:87	Transdermal delivery of sinapine thiocyanate by gelatin microspheres and hyaluronic acid microneedles for allergic asthma in guinea pigs.					
35710072	6	34	theme	pharmacokinetic	931:945	arg1	tests					947:951	in vivo pharmacodynamic and pharmacokinetic tests	903:951	in vivo pharmacodynamic and pharmacokinetic tests	903:951	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	5	35	theme	isothiocyanate	676:689	arg1	staining					691:698	fluorescein isothiocyanate staining	664:698	fluorescein isothiocyanate staining	664:698	In vitro, skin implantation ability was assessed with fluorescein isothiocyanate staining, which revealed progressive penetration from the puncture site into deeper tissues.					
35710072	6	36	theme	MNs	840:842	arg1	delivery					815:822	transdermal delivery	803:822	transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs	803:873	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	6	37	from	delivery	815:822	arg1	pigs					870:873	allergic asthma guinea pigs	847:873	allergic asthma guinea pigs	847:873	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	6	38	from	pigs	870:873	arg1	feasibility					788:798	The feasibility	784:798	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs	784:873	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	7	39	theme	allergic	1189:1196	arg1	therapy					1205:1211	allergic asthma therapy	1189:1211	allergic asthma therapy	1189:1211	The results indicated that ST-GMS/ST-HA MNs, in comparison with the traditional subcutaneous application approach, achieved both high efficiency and continuous release of ST. Therefore, this device is promising for the delivery ST for allergic asthma therapy.					
35710072	5	40	theme	skin	620:623	arg1	ability					638:644	skin implantation ability	620:644	skin implantation ability	620:644	In vitro, skin implantation ability was assessed with fluorescein isothiocyanate staining, which revealed progressive penetration from the puncture site into deeper tissues.					
35710072	6	41	theme	ST-GMS/ST-HA	827:838	arg1	MNs					840:842	ST-GMS/ST-HA MNs	827:842	ST-GMS/ST-HA MNs	827:842	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	3	42	used	used	446:449	arg2	we					443:444	we	443:444	we	443:444	To immobilize ST in MNs, we used a two-step centrifuging and molding method.					
35710072	2	43	theme	thiocyanate	341:351	arg1	GMS					387:389	GMS	387:389	GMS	387:389	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	2	43	theme	thiocyanate	341:351	arg1	microspheres					373:384	sinapine thiocyanate (ST)-loaded gelatin microspheres	332:384	sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS)	332:390	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	0	44	theme	gelatin	48:54	arg1	microspheres					56:67	gelatin microspheres	48:67	gelatin microspheres	48:67	Transdermal delivery of sinapine thiocyanate by gelatin microspheres and hyaluronic acid microneedles for allergic asthma in guinea pigs.					
35710072	6	45	theme	pharmacodynamic	911:925	arg1	tests					947:951	in vivo pharmacodynamic and pharmacokinetic tests	903:951	in vivo pharmacodynamic and pharmacokinetic tests	903:951	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	5	46	theme	implantation	625:636	arg1	ability					638:644	skin implantation ability	620:644	skin implantation ability	620:644	In vitro, skin implantation ability was assessed with fluorescein isothiocyanate staining, which revealed progressive penetration from the puncture site into deeper tissues.					
35710072	6	47	theme	delivery	815:822	arg1	feasibility					788:798	The feasibility	784:798	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs	784:873	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	2	48	theme	sinapine	332:339	arg1	GMS					387:389	GMS	387:389	GMS	387:389	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	2	48	theme	sinapine	332:339	arg1	microspheres					373:384	sinapine thiocyanate (ST)-loaded gelatin microspheres	332:384	sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS)	332:390	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	0	49	from	asthma	115:120	arg1	pigs					132:135	guinea pigs	125:135	guinea pigs	125:135	Transdermal delivery of sinapine thiocyanate by gelatin microspheres and hyaluronic acid microneedles for allergic asthma in guinea pigs.					
35710072	2	50	dep	microspheres	373:384	arg1	ST					354:355	ST	354:355	ST	354:355	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	1	51	theme	painless	206:213	arg1	method					215:220	an efficient, safe, and generally painless method	172:220	an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines	172:279	Dissolving microneedles (MNs) are an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines.					
35710072	1	51	theme	painless	206:213	arg1	microneedles					149:160	Dissolving microneedles	138:160	Dissolving microneedles (MNs)	138:166	Dissolving microneedles (MNs) are an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines.					
35710072	7	52	theme	traditional	1022:1032	arg1	approach					1059:1066	the traditional subcutaneous application approach	1018:1066	the traditional subcutaneous application approach	1018:1066	The results indicated that ST-GMS/ST-HA MNs, in comparison with the traditional subcutaneous application approach, achieved both high efficiency and continuous release of ST. Therefore, this device is promising for the delivery ST for allergic asthma therapy.					
35710072	6	53	theme	transdermal	803:813	arg1	delivery					815:822	transdermal delivery	803:822	transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs	803:873	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	7	54	theme	ST-GMS/ST-HA	981:992	arg1	MNs					994:996	ST-GMS/ST-HA MNs	981:996	ST-GMS/ST-HA MNs	981:996	The results indicated that ST-GMS/ST-HA MNs, in comparison with the traditional subcutaneous application approach, achieved both high efficiency and continuous release of ST. Therefore, this device is promising for the delivery ST for allergic asthma therapy.					
35710072	1	55	theme	Dissolving	138:147	arg1	MNs					163:165	MNs	163:165	MNs	163:165	Dissolving microneedles (MNs) are an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines.					
35710072	1	55	theme	Dissolving	138:147	arg1	method					215:220	an efficient, safe, and generally painless method	172:220	an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines	172:279	Dissolving microneedles (MNs) are an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines.					
35710072	1	55	theme	Dissolving	138:147	arg1	microneedles					149:160	Dissolving microneedles	138:160	Dissolving microneedles (MNs)	138:166	Dissolving microneedles (MNs) are an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines.					
35710072	0	56	theme	hyaluronic	73:82	arg1	acid					84:87	hyaluronic acid	73:87	hyaluronic acid	73:87	Transdermal delivery of sinapine thiocyanate by gelatin microspheres and hyaluronic acid microneedles for allergic asthma in guinea pigs.					
35710072	2	57	theme	-loaded	357:363	arg1	GMS					387:389	GMS	387:389	GMS	387:389	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	2	57	theme	-loaded	357:363	arg1	microspheres					373:384	sinapine thiocyanate (ST)-loaded gelatin microspheres	332:384	sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS)	332:390	Here, dissolving composite MNs were prepared from sinapine thiocyanate (ST)-loaded gelatin microspheres (GMS) and hyaluronic acid (HA).					
35710072	4	58	theme	mechanical	568:577	arg1	strength					579:586	extraordinary mechanical strength	554:586	extraordinary mechanical strength	554:586	When ST-GMS/ST-HA MNs were placed on the skin, they showed extraordinary mechanical strength and dissolved slowly.					
35710072	7	59	with	comparison	1002:1011	arg1	approach					1059:1066	the traditional subcutaneous application approach	1018:1066	the traditional subcutaneous application approach	1018:1066	The results indicated that ST-GMS/ST-HA MNs, in comparison with the traditional subcutaneous application approach, achieved both high efficiency and continuous release of ST. Therefore, this device is promising for the delivery ST for allergic asthma therapy.					
35710072	6	60	theme	in	903:904	arg1	tests					947:951	in vivo pharmacodynamic and pharmacokinetic tests	903:951	in vivo pharmacodynamic and pharmacokinetic tests	903:951	The feasibility of transdermal delivery of ST-GMS/ST-HA MNs in allergic asthma guinea pigs was then determined through in vivo pharmacodynamic and pharmacokinetic tests.					
35710072	4	61	theme	extraordinary	554:566	arg1	strength					579:586	extraordinary mechanical strength	554:586	extraordinary mechanical strength	554:586	When ST-GMS/ST-HA MNs were placed on the skin, they showed extraordinary mechanical strength and dissolved slowly.					
35710072	1	62	theme	transdermal	226:236	arg1	distribution					238:249	transdermal distribution	226:249	transdermal distribution of poorly permeable medicines	226:279	Dissolving microneedles (MNs) are an efficient, safe, and generally painless method for transdermal distribution of poorly permeable medicines.					
35023809	7	0	theme	correct	1313:1319	arg1	answers					1321:1327	correct answers	1313:1327	correct answers	1313:1327	Overall, the highest percentage of correct answers was obtained when examining urine samples containing Escherichia coli: Microscopy (78.3%), culture (87.0%) and susceptibility testing (range: 84.3-90.7%).					
35023809	11	1	theme	used	1899:1902	arg1	microscopy					1874:1883	microscopy	1874:1883	microscopy	1874:1883	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	11	1	theme	used	1899:1902	arg1	method					1904:1909	the least used method	1889:1909	the least used method	1889:1909	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	2	2	theme	Descriptive	317:327	arg1	studySetting					329:340	Descriptive studySetting	317:340	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).	309:555	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	4	3	theme	Flexicult	817:825	arg1	kittm					839:843	Flexicult SSI urinary kittm	817:843	Flexicult SSI urinary kittm (87.6%)	817:851	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	4	3	theme	Flexicult	817:825	arg1	%					850:850	87.6%	846:850	87.6%	846:850	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	11	4	theme	general	2070:2076	arg1	practice					2078:2085	general practice	2070:2085	general practice	2070:2085	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	5	5	theme	strains	1099:1105	arg1	testing					1073:1079	susceptibility testing	1058:1079	susceptibility testing of most bacterial strains (84.6%)	1058:1113	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	2	6	contain	containing	439:448	arg1	samples					431:437	Simulated urine samples	415:437	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).	309:555	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	2	6	contain	containing	439:448	arg2	bacteria					464:471	uropathogenic bacteria	450:471	uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP)	450:554	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	10	7	theme	only	1759:1762	arg1	decision					1747:1754	the treatment decision	1733:1754	the treatment decision	1733:1754	This approach may compromise the treatment decision as only cultures contribute with information about the flora composition and bacterial quantification.					
35023809	10	7	theme	only	1759:1762	arg1	cultures					1764:1771	only cultures	1759:1771	only cultures contribute with information about the flora composition and bacterial quantification	1759:1856	This approach may compromise the treatment decision as only cultures contribute with information about the flora composition and bacterial quantification.					
35023809	1	8	from	practice	262:269	arg1	2013					296:299	2013	296:299	2013	296:299	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	1	8	from	practice	262:269	arg1	Denmark					283:289	Northern Denmark	274:289	Northern Denmark from 2013 to 2018	274:307	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	6	9	with	testing	1175:1181	arg1	tablets					1188:1194	tablets	1188:1194	tablets (range: 76.1-84.6%)	1188:1214	Furthermore, susceptibility testing with tablets (range: 76.1-84.6%) was found to be more accurate than discs (range: 72.9-75.5%).					
35023809	1	10	theme	point-of-care	168:180	arg1	microscopy					188:197	point-of-care (POC) microscopy	168:197	point-of-care (POC) microscopy	168:197	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	6	11	dep	tablets	1188:1194	arg1	range					1197:1201	range	1197:1201	range: 76.1-84.6%	1197:1213	Furthermore, susceptibility testing with tablets (range: 76.1-84.6%) was found to be more accurate than discs (range: 72.9-75.5%).					
35023809	7	12	theme	susceptibility	1440:1453	arg1	testing					1455:1461	susceptibility testing	1440:1461	susceptibility testing (range: 84.3-90.7%)	1440:1481	Overall, the highest percentage of correct answers was obtained when examining urine samples containing Escherichia coli: Microscopy (78.3%), culture (87.0%) and susceptibility testing (range: 84.3-90.7%).					
35023809	3	13	theme	outcome	561:567	arg1	measures					569:576	Main outcome measures	556:576	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).	556:802	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	1	14	theme	POC	183:185	arg1	microscopy					188:197	point-of-care (POC) microscopy	168:197	point-of-care (POC) microscopy	168:197	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	6	15	dep	range	1197:1201	arg1	%					1213:1213	76.1-84.6%	1204:1213	range: 76.1-84.6%	1197:1213	Furthermore, susceptibility testing with tablets (range: 76.1-84.6%) was found to be more accurate than discs (range: 72.9-75.5%).					
35023809	5	16	theme	Mueller	975:981	arg1	agar					990:993	Mueller Hinton agar	975:993	Mueller Hinton agar with tablets	975:1006	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	4	17	theme	2-plate	949:955	arg1	dipslide					957:964	2-plate dipslide	949:964	2-plate dipslide (85.2%)	949:972	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	4	17	theme	2-plate	949:955	arg1	%					971:971	85.2%	967:971	85.2%	967:971	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	1	18	theme	microscopy	188:197	arg1	quality					157:163	quality	157:163	quality	157:163	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	1	18	theme	microscopy	188:197	arg1	use					149:151	use	149:151	use	149:151	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	4	19	theme	urinary	831:837	arg1	kittm					839:843	Flexicult SSI urinary kittm	817:843	Flexicult SSI urinary kittm (87.6%)	817:851	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	4	19	theme	urinary	831:837	arg1	%					850:850	87.6%	846:850	87.6%	846:850	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	11	20	theme	urine	2330:2334	arg1	cultures					2336:2343	only urine cultures	2325:2343	only urine cultures	2325:2343	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	11	21	theme	pneumoniae.Susceptibility	2217:2241	arg1	testing					2243:2249	pneumoniae.Susceptibility testing	2217:2249	pneumoniae.Susceptibility testing	2217:2249	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	6	22	theme	susceptibility	1160:1173	arg1	testing					1175:1181	susceptibility testing	1160:1181	susceptibility testing with tablets (range: 76.1-84.6%)	1160:1214	Furthermore, susceptibility testing with tablets (range: 76.1-84.6%) was found to be more accurate than discs (range: 72.9-75.5%).					
35023809	4	23	theme	answers	900:906	arg1	percentage					878:887	the highest percentage	866:887	the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%)	866:972	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	11	24	contain	containing	2117:2126	arg1	urine					2111:2115	urine	2111:2115	urine containing E. coli	2111:2134	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	11	24	contain	containing	2117:2126	arg2	coli					2131:2134	E. coli	2128:2134	E. coli	2128:2134	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	0	25	theme	culture	51:57	arg1	quality					8:14	quality	8:14	quality	8:14	Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.					
35023809	0	25	theme	culture	51:57	arg1	Use					0:2	Use	0:2	Use	0:2	Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.					
35023809	0	26	from	quality	8:14	arg1	practice					112:119	general practice	104:119	general practice	104:119	Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.					
35023809	3	27	theme	testing	787:793	arg1	use					722:724	the use	718:724	the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%)	718:801	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	0	28	theme	susceptibility	63:76	arg1	testing					78:84	susceptibility testing	63:84	susceptibility testing	63:84	Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.					
35023809	2	29	theme	quality	540:546	arg1	improvement					509:519	improvement	509:519	improvement of microbiological quality (MIKAP)	509:554	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	0	30	from	Use	0:2	arg1	practice					112:119	general practice	104:119	general practice	104:119	Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.					
35023809	10	31	theme	bacterial	1833:1841	arg1	quantification					1843:1856	bacterial quantification	1833:1856	bacterial quantification	1833:1856	This approach may compromise the treatment decision as only cultures contribute with information about the flora composition and bacterial quantification.					
35023809	8	32	theme	common	1600:1605	arg1	uropathogen					1607:1617	the most common uropathogen	1591:1617	the most common uropathogen E. coli	1591:1625	Conclusion: The quality of POC testing in general practice was high when examining urine samples containing the most common uropathogen E. coli.					
35023809	8	33	theme	E.	1619:1620	arg1	uropathogen					1607:1617	the most common uropathogen	1591:1617	the most common uropathogen E. coli	1591:1625	Conclusion: The quality of POC testing in general practice was high when examining urine samples containing the most common uropathogen E. coli.					
35023809	11	34	theme	minutes.Key	1962:1972	arg1	pointsThe					1974:1982	a few minutes.Key pointsThe	1956:1982	a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification	1956:2428	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	5	35	theme	bacterial	1089:1097	arg1	strains					1099:1105	most bacterial strains	1084:1105	most bacterial strains (84.6%)	1084:1113	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	5	35	theme	bacterial	1089:1097	arg1	%					1112:1112	84.6%	1108:1112	84.6%	1108:1112	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	11	36	theme	susceptibility	2033:2046	arg1	microscopy					2006:2015	microscopy	2006:2015	microscopy	2006:2015	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	11	36	theme	susceptibility	2033:2046	arg1	testing					2048:2054	susceptibility testing	2033:2054	susceptibility testing	2033:2054	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	1	37	theme	general	254:260	arg1	practice					262:269	general practice	254:269	general practice in Northern Denmark from 2013 to 2018	254:307	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	2	38	theme	Simulated	415:423	arg1	samples					431:437	Simulated urine samples	415:437	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).	309:555	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	5	39	contain	had	1008:1010	arg1	agar					990:993	Mueller Hinton agar	975:993	Mueller Hinton agar with tablets	975:1006	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	5	39	contain	had	1008:1010	arg2	percentage					1024:1033	the highest percentage	1012:1033	the highest percentage of correct answers	1012:1052	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	5	40	theme	most	1084:1087	arg1	strains					1099:1105	most bacterial strains	1084:1105	most bacterial strains (84.6%)	1084:1113	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	5	40	theme	most	1084:1087	arg1	%					1112:1112	84.6%	1108:1112	84.6%	1108:1112	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	11	41	theme	POC	1995:1997	arg1	tests					1999:2003	POC tests	1995:2003	POC tests (microscopy, urine culture, susceptibility testing) performed in general practice	1995:2085	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	3	42	theme	susceptibility	645:658	arg1	testing.Results					660:674	susceptibility testing.Results	645:674	susceptibility testing.Results	645:674	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	8	43	from	quality	1499:1505	arg1	practice					1533:1540	general practice	1525:1540	general practice	1525:1540	Conclusion: The quality of POC testing in general practice was high when examining urine samples containing the most common uropathogen E. coli.					
35023809	0	44	theme	point-of-care	19:31	arg1	microscopy					33:42	point-of-care microscopy	19:42	point-of-care microscopy	19:42	Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.					
35023809	5	45	theme	correct	1038:1044	arg1	answers					1046:1052	correct answers	1038:1052	correct answers	1038:1052	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	11	46	theme	tests	1999:2003	arg1	quality					1984:1990	quality	1984:1990	quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice	1984:2085	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	2	47	theme	uropathogenic	450:462	arg1	bacteria					464:471	uropathogenic bacteria	450:471	uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP)	450:554	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	11	48	theme	urine	2018:2022	arg1	microscopy					2006:2015	microscopy	2006:2015	microscopy	2006:2015	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	11	48	theme	urine	2018:2022	arg1	culture					2024:2030	urine culture	2018:2030	urine culture	2018:2030	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	7	49	theme	answers	1321:1327	arg1	percentage					1299:1308	the highest percentage	1287:1308	the highest percentage of correct answers	1287:1327	Overall, the highest percentage of correct answers was obtained when examining urine samples containing Escherichia coli: Microscopy (78.3%), culture (87.0%) and susceptibility testing (range: 84.3-90.7%).					
35023809	9	50	theme	susceptibility	1642:1655	arg1	testing					1657:1663	susceptibility testing	1642:1663	susceptibility testing	1642:1663	Surprisingly, susceptibility testing was more frequently used than culture.					
35023809	11	51	theme	bacterial	2405:2413	arg1	quantification					2415:2428	bacterial quantification	2405:2428	bacterial quantification	2405:2428	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	2	52	theme	General	343:349	arg1	practices					351:359	General practices	343:359	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).	309:555	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	1	53	from	2013	296:299	arg1	practice					262:269	general practice	254:269	general practice in Northern Denmark from 2013 to 2018	254:307	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	1	53	from	2013	296:299	arg1	Denmark					283:289	Northern Denmark	274:289	Northern Denmark from 2013 to 2018	274:307	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	1	54	theme	susceptibility	218:231	arg1	testing					233:239	susceptibility testing	218:239	susceptibility testing	218:239	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	7	55	theme	highest	1291:1297	arg1	percentage					1299:1308	the highest percentage	1287:1308	the highest percentage of correct answers	1287:1327	Overall, the highest percentage of correct answers was obtained when examining urine samples containing Escherichia coli: Microscopy (78.3%), culture (87.0%) and susceptibility testing (range: 84.3-90.7%).					
35023809	3	56	theme	5361	688:691	arg1	samples					693:699	5361 samples	688:699	5361 samples	688:699	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	8	57	contain	containing	1580:1589	arg2	uropathogen					1607:1617	the most common uropathogen	1591:1617	the most common uropathogen E. coli	1591:1625	Conclusion: The quality of POC testing in general practice was high when examining urine samples containing the most common uropathogen E. coli.					
35023809	8	57	contain	containing	1580:1589	arg1	samples					1572:1578	urine samples	1566:1578	urine samples containing the most common uropathogen E. coli	1566:1625	Conclusion: The quality of POC testing in general practice was high when examining urine samples containing the most common uropathogen E. coli.					
35023809	8	58	dep	E.	1619:1620	arg1	coli					1622:1625	coli	1622:1625	coli	1622:1625	Conclusion: The quality of POC testing in general practice was high when examining urine samples containing the most common uropathogen E. coli.					
35023809	11	59	theme	urine	2281:2285	arg1	culture					2287:2293	urine culture	2281:2293	urine culture	2281:2293	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	3	60	theme	Main	556:559	arg1	measures					569:576	Main outcome measures	556:576	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).	556:802	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	8	61	theme	POC	1510:1512	arg1	testing					1514:1520	POC testing	1510:1520	POC testing	1510:1520	Conclusion: The quality of POC testing in general practice was high when examining urine samples containing the most common uropathogen E. coli.					
35023809	3	62	theme	microscopy	729:738	arg1	use					722:724	the use	718:724	the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%)	718:801	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	3	63	theme	use	593:595	arg1	answers					609:615	correct answers	601:615	correct answers for microscopy, culture and susceptibility testing.Results	601:674	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	3	63	theme	use	593:595	arg1	Percentage					579:588	Percentage	579:588	Percentage of use	579:595	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	8	64	theme	testing	1514:1520	arg1	quality					1499:1505	The quality	1495:1505	The quality of POC testing in general practice	1495:1540	Conclusion: The quality of POC testing in general practice was high when examining urine samples containing the most common uropathogen E. coli.					
35023809	8	64	theme	testing	1514:1520	arg1	high					1546:1549	high	1546:1549	high	1546:1549	Conclusion: The quality of POC testing in general practice was high when examining urine samples containing the most common uropathogen E. coli.					
35023809	7	65	contain	containing	1371:1380	arg1	samples					1363:1369	urine samples	1357:1369	urine samples containing Escherichia coli	1357:1397	Overall, the highest percentage of correct answers was obtained when examining urine samples containing Escherichia coli: Microscopy (78.3%), culture (87.0%) and susceptibility testing (range: 84.3-90.7%).					
35023809	7	65	contain	containing	1371:1380	arg2	coli					1394:1397	Escherichia coli	1382:1397	Escherichia coli	1382:1397	Overall, the highest percentage of correct answers was obtained when examining urine samples containing Escherichia coli: Microscopy (78.3%), culture (87.0%) and susceptibility testing (range: 84.3-90.7%).					
35023809	4	66	theme	highest	870:876	arg1	percentage					878:887	the highest percentage	866:887	the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%)	866:972	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	5	67	theme	Hinton	983:988	arg1	agar					990:993	Mueller Hinton agar	975:993	Mueller Hinton agar with tablets	975:1006	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	8	68	theme	general	1525:1531	arg1	practice					1533:1540	general practice	1525:1540	general practice	1525:1540	Conclusion: The quality of POC testing in general practice was high when examining urine samples containing the most common uropathogen E. coli.					
35023809	4	69	theme	SSI	827:829	arg1	kittm					839:843	Flexicult SSI urinary kittm	817:843	Flexicult SSI urinary kittm (87.6%)	817:851	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	4	69	theme	SSI	827:829	arg1	%					850:850	87.6%	846:850	87.6%	846:850	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	10	70	with	cultures	1764:1771	arg1	information					1789:1799	information	1789:1799	information about the flora composition and bacterial quantification	1789:1856	This approach may compromise the treatment decision as only cultures contribute with information about the flora composition and bacterial quantification.					
35023809	7	71	dep	testing	1455:1461	arg1	range					1464:1468	range	1464:1468	range: 84.3-90.7%	1464:1480	Overall, the highest percentage of correct answers was obtained when examining urine samples containing Escherichia coli: Microscopy (78.3%), culture (87.0%) and susceptibility testing (range: 84.3-90.7%).					
35023809	5	72	with	agar	990:993	arg1	tablets					1000:1006	tablets	1000:1006	tablets	1000:1006	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	10	73	theme	flora	1811:1815	arg1	composition					1817:1827	the flora composition	1807:1827	the flora composition	1807:1827	This approach may compromise the treatment decision as only cultures contribute with information about the flora composition and bacterial quantification.					
35023809	3	74	theme	correct	601:607	arg1	answers					609:615	correct answers	601:615	correct answers for microscopy, culture and susceptibility testing.Results	601:674	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	4	75	theme	correct	892:898	arg1	answers					900:906	correct answers	892:906	correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%)	892:972	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	7	76	dep	range	1464:1468	arg1	%					1480:1480	84.3-90.7%	1471:1480	range: 84.3-90.7%	1464:1480	Overall, the highest percentage of correct answers was obtained when examining urine samples containing Escherichia coli: Microscopy (78.3%), culture (87.0%) and susceptibility testing (range: 84.3-90.7%).					
35023809	0	77	theme	testing	78:84	arg1	quality					8:14	quality	8:14	quality	8:14	Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.					
35023809	0	77	theme	testing	78:84	arg1	Use					0:2	Use	0:2	Use	0:2	Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.					
35023809	5	78	theme	highest	1016:1022	arg1	percentage					1024:1033	the highest percentage	1012:1033	the highest percentage of correct answers	1012:1052	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	8	79	theme	urine	1566:1570	arg1	samples					1572:1578	urine samples	1566:1578	urine samples containing the most common uropathogen E. coli	1566:1625	Conclusion: The quality of POC testing in general practice was high when examining urine samples containing the most common uropathogen E. coli.					
35023809	3	80	dep	measures	569:576	arg1	answers					609:615	correct answers	601:615	correct answers for microscopy, culture and susceptibility testing.Results	601:674	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	3	80	dep	measures	569:576	arg1	Percentage					579:588	Percentage	579:588	Percentage of use	579:595	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	3	80	dep	measures	569:576	arg1	analysed					706:713	analysed	706:713	were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%)	701:801	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	10	81	dep	information	1789:1799	arg1	contribute					1773:1782	contribute	1773:1782	contribute	1773:1782	This approach may compromise the treatment decision as only cultures contribute with information about the flora composition and bacterial quantification.					
35023809	11	82	dep	saprophyticus	2197:2209	arg1	performed					2266:2274	performed	2266:2274	was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification	2251:2428	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	2	83	theme	microbiological	524:538	arg1	MIKAP					549:553	MIKAP	549:553	MIKAP	549:553	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	2	83	theme	microbiological	524:538	arg1	quality					540:546	microbiological quality	524:546	microbiological quality (MIKAP)	524:554	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	11	84	dep	pointsThe	1974:1982	arg1	high					2091:2094	high	2091:2094	high	2091:2094	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	3	85	theme	susceptibility	772:785	arg1	testing					787:793	susceptibility testing	772:793	susceptibility testing (76.5%)	772:801	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	3	85	theme	susceptibility	772:785	arg1	%					800:800	76.5%	796:800	76.5%	796:800	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	0	86	theme	general	104:110	arg1	practice					112:119	general practice	104:119	general practice	104:119	Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.					
35023809	10	87	theme	treatment	1737:1745	arg1	decision					1747:1754	the treatment decision	1733:1754	the treatment decision	1733:1754	This approach may compromise the treatment decision as only cultures contribute with information about the flora composition and bacterial quantification.					
35023809	10	87	theme	treatment	1737:1745	arg1	cultures					1764:1771	only cultures	1759:1771	only cultures contribute with information about the flora composition and bacterial quantification	1759:1856	This approach may compromise the treatment decision as only cultures contribute with information about the flora composition and bacterial quantification.					
35023809	2	88	theme	urine	425:429	arg1	samples					431:437	Simulated urine samples	415:437	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).	309:555	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	11	89	dep	tests	1999:2003	arg1	microscopy					2006:2015	microscopy	2006:2015	microscopy	2006:2015	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	11	89	dep	tests	1999:2003	arg1	culture					2024:2030	urine culture	2018:2030	urine culture	2018:2030	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	11	89	dep	tests	1999:2003	arg1	testing					2048:2054	susceptibility testing	2033:2054	susceptibility testing	2033:2054	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	1	90	dep	use	149:151	arg1	the					145:147	the	145:147	the	145:147	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	6	91	dep	discs	1251:1255	arg1	range					1258:1262	range	1258:1262	range: 72.9-75.5%	1258:1274	Furthermore, susceptibility testing with tablets (range: 76.1-84.6%) was found to be more accurate than discs (range: 72.9-75.5%).					
35023809	11	92	theme	few	1958:1960	arg1	pointsThe					1974:1982	a few minutes.Key pointsThe	1956:1982	a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification	1956:2428	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	2	93	dep	Design	309:314	arg1	studySetting					329:340	Descriptive studySetting	317:340	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).	309:555	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	2	93	dep	Design	309:314	arg1	practices					351:359	General practices	343:359	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).	309:555	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	2	93	dep	Design	309:314	arg1	samples					431:437	Simulated urine samples	415:437	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).	309:555	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	2	94	theme	urine	391:395	arg1	samples.Subjects					397:412	urine samples.Subjects	391:412	urine samples.Subjects	391:412	Design: Descriptive studySetting: General practices receiving a fee for examining urine samples.Subjects: Simulated urine samples containing uropathogenic bacteria distributed by the organisation for improvement of microbiological quality (MIKAP).					
35023809	0	95	theme	microscopy	33:42	arg1	quality					8:14	quality	8:14	quality	8:14	Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.					
35023809	0	95	theme	microscopy	33:42	arg1	Use					0:2	Use	0:2	Use	0:2	Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.					
35023809	1	96	theme	Northern	274:281	arg1	Denmark					283:289	Northern Denmark	274:289	Northern Denmark from 2013 to 2018	274:307	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	6	97	dep	range	1258:1262	arg1	%					1274:1274	72.9-75.5%	1265:1274	range: 72.9-75.5%	1258:1274	Furthermore, susceptibility testing with tablets (range: 76.1-84.6%) was found to be more accurate than discs (range: 72.9-75.5%).					
35023809	4	98	theme	chromogenic	920:930	arg1	agar					932:935	chromogenic agar	920:935	chromogenic agar (85.1%)	920:943	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	4	98	theme	chromogenic	920:930	arg1	%					942:942	85.1%	938:942	85.1%	938:942	For culture, Flexicult SSI urinary kittm (87.6%) demonstrated the highest percentage of correct answers followed by chromogenic agar (85.1%) and 2-plate dipslide (85.2%).					
35023809	0	99	theme	urine	45:49	arg1	culture					51:57	urine culture	45:57	urine culture	45:57	Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.					
35023809	5	100	theme	answers	1046:1052	arg1	percentage					1024:1033	the highest percentage	1012:1033	the highest percentage of correct answers	1012:1052	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	7	101	theme	urine	1357:1361	arg1	samples					1363:1369	urine samples	1357:1369	urine samples containing Escherichia coli	1357:1397	Overall, the highest percentage of correct answers was obtained when examining urine samples containing Escherichia coli: Microscopy (78.3%), culture (87.0%) and susceptibility testing (range: 84.3-90.7%).					
35023809	9	102	used	used	1685:1688	arg2	testing					1657:1663	susceptibility testing	1642:1663	susceptibility testing	1642:1663	Surprisingly, susceptibility testing was more frequently used than culture.					
35023809	5	103	theme	susceptibility	1058:1071	arg1	testing					1073:1079	susceptibility testing	1058:1079	susceptibility testing of most bacterial strains (84.6%)	1058:1113	Mueller Hinton agar with tablets had the highest percentage of correct answers for susceptibility testing of most bacterial strains (84.6%), followed by Flexicult (77.2%).					
35023809	1	104	theme	urine	200:204	arg1	culture					206:212	urine culture	200:212	urine culture	200:212	Objective: To describe the use and quality of point-of-care (POC) microscopy, urine culture and susceptibility testing performed in general practice in Northern Denmark from 2013 to 2018.					
35023809	11	105	theme	flora	2383:2387	arg1	composition					2389:2399	the flora composition	2379:2399	the flora composition	2379:2399	Interestingly, microscopy was the least used method even though the result may be reached within a few minutes.Key pointsThe quality of POC tests (microscopy, urine culture, susceptibility testing) performed in general practice was high when examining urine containing E. coli, whereas difficulties were observed for samples including S. saprophyticus or K. pneumoniae.Susceptibility testing was more often performed than urine culture, which indicates a problem as only urine cultures contribute with information about the flora composition and bacterial quantification.					
35023809	3	106	theme	samples	693:699	arg1	total					679:683	A total	677:683	A total of 5361 samples	677:699	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
35023809	3	107	theme	culture	749:755	arg1	use					722:724	the use	718:724	the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%)	718:801	Main outcome measures: Percentage of use and correct answers for microscopy, culture and susceptibility testing.Results: A total of 5361 samples were analysed by the use of microscopy (39.7%), culture (66.0%) and/or susceptibility testing (76.5%).					
36870659	0	0	theme	cinnamon	112:119	arg1	oil					131:133	cinnamon essential oil	112:133	cinnamon essential oil as an active packaging material	112:165	Preparation and characterization of functionalized chitosan/polyvinyl alcohol composite films incorporated with cinnamon essential oil as an active packaging material.					
36870659	1	1	theme	active	335:340	arg1	film					349:352	an active edible film	332:352	an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method	332:466	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	4	2	theme	film	801:804	arg1	strength					743:750	the tensile strength	731:750	the tensile strength	731:750	And, the tensile strength and elongation at break of the NPCS-CA/PVA (5/5) film with 0.4 % CEO were 20.32 MPa and 65.73 %, respectively.					
36870659	4	2	theme	film	801:804	arg1	20.32 MPa					826:834	20.32 MPa	826:834	20.32 MPa	826:834	And, the tensile strength and elongation at break of the NPCS-CA/PVA (5/5) film with 0.4 % CEO were 20.32 MPa and 65.73 %, respectively.					
36870659	4	2	theme	film	801:804	arg1	elongation					756:765	elongation	756:765	elongation	756:765	And, the tensile strength and elongation at break of the NPCS-CA/PVA (5/5) film with 0.4 % CEO were 20.32 MPa and 65.73 %, respectively.					
36870659	7	3	theme	quality	1417:1423	arg1	indexes					1425:1431	quality indexes	1417:1431	quality indexes	1417:1431	And, the multifunctional films effectively extended the shelf-life of mangoes at 25 °C based on the characterization of surface changes and quality indexes.					
36870659	7	4	theme	indexes	1425:1431	arg1	characterization					1377:1392	the characterization	1373:1392	the characterization of surface changes and quality indexes	1373:1431	And, the multifunctional films effectively extended the shelf-life of mangoes at 25 °C based on the characterization of surface changes and quality indexes.					
36870659	5	5	theme	vapor	1076:1080	arg1	permeability					1082:1093	water vapor permeability	1070:1093	water vapor permeability	1070:1093	The results revealed that the NPCS-CA/PVA-CEO composite films exhibited an excellent ultraviolet barrier property at 200-300 nm and significantly reduced oxygen permeability, carbon dioxide permeability and water vapor permeability.					
36870659	1	6	theme	edible	342:347	arg1	film					349:352	an active edible film	332:352	an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method	332:466	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	5	7	theme	reduced	1009:1015	arg1	permeability					1024:1035	significantly reduced oxygen permeability	995:1035	significantly reduced oxygen permeability	995:1035	The results revealed that the NPCS-CA/PVA-CEO composite films exhibited an excellent ultraviolet barrier property at 200-300 nm and significantly reduced oxygen permeability, carbon dioxide permeability and water vapor permeability.					
36870659	1	8	theme	essential	426:434	arg1	CEO					441:443	CEO	441:443	CEO	441:443	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	1	8	theme	essential	426:434	arg1	oil					436:438	cinnamon essential oil	417:438	cinnamon essential oil (CEO)	417:444	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	1	9	theme	cholic	274:279	arg1	acid					281:284	cholic acid	274:284	cholic acid	274:284	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	2	10	theme	chitosan	499:506	arg1	derivative					508:517	the chitosan derivative	495:517	the chitosan derivative	495:517	The chemical structure of the chitosan derivative was characterized by FT-IR, 1H NMR and XRD.					
36870659	3	11	theme	composite	648:656	arg1	films					658:662	the composite films	644:662	the composite films	644:662	Through the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films, the optimal proportion of NPCS-CA/PVA was determined as 5/5.					
36870659	4	12	theme	0.4 	811:814	arg1	%					815:815	%	815:815	%	815:815	And, the tensile strength and elongation at break of the NPCS-CA/PVA (5/5) film with 0.4 % CEO were 20.32 MPa and 65.73 %, respectively.					
36870659	6	13	theme	solutions	1152:1160	arg1	property					1127:1134	the antibacterial property	1109:1134	the antibacterial property of film-forming solutions against E. coli, S. aureus, and C. lagenarium	1109:1206	Furthermore, the antibacterial property of film-forming solutions against E. coli, S. aureus, and C. lagenarium was distinctly improved with the increase of NPCS-CA/PVA proportion.					
36870659	0	14	theme	essential	121:129	arg1	oil					131:133	cinnamon essential oil	112:133	cinnamon essential oil as an active packaging material	112:165	Preparation and characterization of functionalized chitosan/polyvinyl alcohol composite films incorporated with cinnamon essential oil as an active packaging material.					
36870659	5	15	theme	carbon	1038:1043	arg1	dioxide					1045:1051	carbon dioxide	1038:1051	carbon dioxide permeability	1038:1064	The results revealed that the NPCS-CA/PVA-CEO composite films exhibited an excellent ultraviolet barrier property at 200-300 nm and significantly reduced oxygen permeability, carbon dioxide permeability and water vapor permeability.					
36870659	7	16	theme	changes	1405:1411	arg1	characterization					1377:1392	the characterization	1373:1392	the characterization of surface changes and quality indexes	1373:1431	And, the multifunctional films effectively extended the shelf-life of mangoes at 25 °C based on the characterization of surface changes and quality indexes.					
36870659	6	17	theme	film-forming	1139:1150	arg1	solutions					1152:1160	film-forming solutions	1139:1160	film-forming solutions	1139:1160	Furthermore, the antibacterial property of film-forming solutions against E. coli, S. aureus, and C. lagenarium was distinctly improved with the increase of NPCS-CA/PVA proportion.					
36870659	5	18	theme	dioxide	1045:1051	arg1	permeability					1053:1064	carbon dioxide permeability	1038:1064	carbon dioxide permeability	1038:1064	The results revealed that the NPCS-CA/PVA-CEO composite films exhibited an excellent ultraviolet barrier property at 200-300 nm and significantly reduced oxygen permeability, carbon dioxide permeability and water vapor permeability.					
36870659	4	19	theme	NPCS-CA/PVA	783:793	arg1	film					801:804	the NPCS-CA/PVA (5/5) film	779:804	the NPCS-CA/PVA (5/5) film with 0.4 % CEO	779:819	And, the tensile strength and elongation at break of the NPCS-CA/PVA (5/5) film with 0.4 % CEO were 20.32 MPa and 65.73 %, respectively.					
36870659	1	20	theme	amphiphilic	183:193	arg1	NPCS-CA					205:211	NPCS-CA	205:211	NPCS-CA	205:211	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	1	20	theme	amphiphilic	183:193	arg1	chitosan					195:202	amphiphilic chitosan	183:202	amphiphilic chitosan (NPCS-CA)	183:212	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	6	21	theme	proportion	1265:1274	arg1	increase					1241:1248	the increase	1237:1248	the increase of NPCS-CA/PVA proportion	1237:1274	Furthermore, the antibacterial property of film-forming solutions against E. coli, S. aureus, and C. lagenarium was distinctly improved with the increase of NPCS-CA/PVA proportion.					
36870659	5	22	theme	water	1070:1074	arg1	permeability					1082:1093	water vapor permeability	1070:1093	water vapor permeability	1070:1093	The results revealed that the NPCS-CA/PVA-CEO composite films exhibited an excellent ultraviolet barrier property at 200-300 nm and significantly reduced oxygen permeability, carbon dioxide permeability and water vapor permeability.					
36870659	8	23	theme	packaging	1500:1508	arg1	films					1454:1458	The NPCS-CA/PVA-CEO films	1434:1458	The NPCS-CA/PVA-CEO films	1434:1458	The NPCS-CA/PVA-CEO films could be developed as biocomposite food packaging material.					
36870659	8	23	theme	packaging	1500:1508	arg1	material					1510:1517	biocomposite food packaging material	1482:1517	biocomposite food packaging material	1482:1517	The NPCS-CA/PVA-CEO films could be developed as biocomposite food packaging material.					
36870659	5	24	theme	NPCS-CA/PVA-CEO	893:907	arg1	films					919:923	the NPCS-CA/PVA-CEO composite films	889:923	the NPCS-CA/PVA-CEO composite films	889:923	The results revealed that the NPCS-CA/PVA-CEO composite films exhibited an excellent ultraviolet barrier property at 200-300 nm and significantly reduced oxygen permeability, carbon dioxide permeability and water vapor permeability.					
36870659	7	25	theme	multifunctional	1286:1300	arg1	films					1302:1306	the multifunctional films	1282:1306	the multifunctional films	1282:1306	And, the multifunctional films effectively extended the shelf-life of mangoes at 25 °C based on the characterization of surface changes and quality indexes.					
36870659	3	26	theme	optimal	669:675	arg1	proportion					677:686	the optimal proportion	665:686	the optimal proportion of NPCS-CA/PVA	665:701	Through the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films, the optimal proportion of NPCS-CA/PVA was determined as 5/5.					
36870659	3	26	theme	optimal	669:675	arg1	5/5					721:723	5/5	721:723	5/5	721:723	Through the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films, the optimal proportion of NPCS-CA/PVA was determined as 5/5.					
36870659	6	27	theme	antibacterial	1113:1125	arg1	property					1127:1134	the antibacterial property	1109:1134	the antibacterial property of film-forming solutions against E. coli, S. aureus, and C. lagenarium	1109:1206	Furthermore, the antibacterial property of film-forming solutions against E. coli, S. aureus, and C. lagenarium was distinctly improved with the increase of NPCS-CA/PVA proportion.					
36870659	4	28	theme	%	815:815	arg1	CEO					817:819	0.4 % CEO	811:819	0.4 % CEO	811:819	And, the tensile strength and elongation at break of the NPCS-CA/PVA (5/5) film with 0.4 % CEO were 20.32 MPa and 65.73 %, respectively.					
36870659	8	29	theme	biocomposite	1482:1493	arg1	films					1454:1458	The NPCS-CA/PVA-CEO films	1434:1458	The NPCS-CA/PVA-CEO films	1434:1458	The NPCS-CA/PVA-CEO films could be developed as biocomposite food packaging material.					
36870659	8	29	theme	biocomposite	1482:1493	arg1	material					1510:1517	biocomposite food packaging material	1482:1517	biocomposite food packaging material	1482:1517	The NPCS-CA/PVA-CEO films could be developed as biocomposite food packaging material.					
36870659	7	30	theme	surface	1397:1403	arg1	changes					1405:1411	surface changes	1397:1411	surface changes	1397:1411	And, the multifunctional films effectively extended the shelf-life of mangoes at 25 °C based on the characterization of surface changes and quality indexes.					
36870659	3	31	theme	barrier	622:628	arg1	properties					630:639	mechanical and barrier properties	607:639	properties	630:639	Through the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films, the optimal proportion of NPCS-CA/PVA was determined as 5/5.					
36870659	3	31	theme	barrier	622:628	arg1	FT-IR					595:599	FT-IR	595:599	FT-IR	595:599	Through the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films, the optimal proportion of NPCS-CA/PVA was determined as 5/5.					
36870659	5	32	theme	oxygen	1017:1022	arg1	permeability					1024:1035	significantly reduced oxygen permeability	995:1035	significantly reduced oxygen permeability	995:1035	The results revealed that the NPCS-CA/PVA-CEO composite films exhibited an excellent ultraviolet barrier property at 200-300 nm and significantly reduced oxygen permeability, carbon dioxide permeability and water vapor permeability.					
36870659	0	33	theme	packaging	148:156	arg1	material					158:165	an active packaging material	138:165	an active packaging material	138:165	Preparation and characterization of functionalized chitosan/polyvinyl alcohol composite films incorporated with cinnamon essential oil as an active packaging material.					
36870659	1	34	theme	casting	453:459	arg1	method					461:466	the casting method	449:466	the casting method	449:466	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	7	35	theme	mangoes	1347:1353	arg1	shelf-life					1333:1342	the shelf-life	1329:1342	the shelf-life of mangoes	1329:1353	And, the multifunctional films effectively extended the shelf-life of mangoes at 25 °C based on the characterization of surface changes and quality indexes.					
36870659	3	36	theme	NPCS-CA/PVA	691:701	arg1	proportion					677:686	the optimal proportion	665:686	the optimal proportion of NPCS-CA/PVA	665:701	Through the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films, the optimal proportion of NPCS-CA/PVA was determined as 5/5.					
36870659	3	36	theme	NPCS-CA/PVA	691:701	arg1	5/5					721:723	5/5	721:723	5/5	721:723	Through the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films, the optimal proportion of NPCS-CA/PVA was determined as 5/5.					
36870659	0	37	theme	active	141:146	arg1	material					158:165	an active packaging material	138:165	an active packaging material	138:165	Preparation and characterization of functionalized chitosan/polyvinyl alcohol composite films incorporated with cinnamon essential oil as an active packaging material.					
36870659	1	38	theme	polyvinyl	375:383	arg1	PVA					394:396	PVA	394:396	PVA	394:396	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	1	38	theme	polyvinyl	375:383	arg1	alcohol					385:391	polyvinyl alcohol	375:391	polyvinyl alcohol (PVA)	375:397	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	0	39	theme	chitosan/polyvinyl	51:68	arg1	films					88:92	functionalized chitosan/polyvinyl alcohol composite films	36:92	functionalized chitosan/polyvinyl alcohol composite films	36:92	Preparation and characterization of functionalized chitosan/polyvinyl alcohol composite films incorporated with cinnamon essential oil as an active packaging material.					
36870659	1	40	theme	chitosan	304:311	arg1	chain					295:299	the chain	291:299	the chain of chitosan	291:311	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	3	41	theme	mechanical	607:616	arg1	properties					630:639	mechanical and barrier properties	607:639	properties	630:639	Through the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films, the optimal proportion of NPCS-CA/PVA was determined as 5/5.					
36870659	3	41	theme	mechanical	607:616	arg1	FT-IR					595:599	FT-IR	595:599	FT-IR	595:599	Through the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films, the optimal proportion of NPCS-CA/PVA was determined as 5/5.					
36870659	5	42	theme	excellent	938:946	arg1	property					968:975	an excellent ultraviolet barrier property	935:975	an excellent ultraviolet barrier property	935:975	The results revealed that the NPCS-CA/PVA-CEO composite films exhibited an excellent ultraviolet barrier property at 200-300 nm and significantly reduced oxygen permeability, carbon dioxide permeability and water vapor permeability.					
36870659	0	43	theme	functionalized	36:49	arg1	films					88:92	functionalized chitosan/polyvinyl alcohol composite films	36:92	functionalized chitosan/polyvinyl alcohol composite films	36:92	Preparation and characterization of functionalized chitosan/polyvinyl alcohol composite films incorporated with cinnamon essential oil as an active packaging material.					
36870659	2	44	theme	derivative	508:517	arg1	structure					482:490	The chemical structure	469:490	The chemical structure of the chitosan derivative	469:517	The chemical structure of the chitosan derivative was characterized by FT-IR, 1H NMR and XRD.					
36870659	5	45	theme	ultraviolet	948:958	arg1	property					968:975	an excellent ultraviolet barrier property	935:975	an excellent ultraviolet barrier property	935:975	The results revealed that the NPCS-CA/PVA-CEO composite films exhibited an excellent ultraviolet barrier property at 200-300 nm and significantly reduced oxygen permeability, carbon dioxide permeability and water vapor permeability.					
36870659	0	46	theme	composite	78:86	arg1	films					88:92	functionalized chitosan/polyvinyl alcohol composite films	36:92	functionalized chitosan/polyvinyl alcohol composite films	36:92	Preparation and characterization of functionalized chitosan/polyvinyl alcohol composite films incorporated with cinnamon essential oil as an active packaging material.					
36870659	5	47	theme	barrier	960:966	arg1	property					968:975	an excellent ultraviolet barrier property	935:975	an excellent ultraviolet barrier property	935:975	The results revealed that the NPCS-CA/PVA-CEO composite films exhibited an excellent ultraviolet barrier property at 200-300 nm and significantly reduced oxygen permeability, carbon dioxide permeability and water vapor permeability.					
36870659	4	48	from	break	770:774	arg1	strength					743:750	the tensile strength	731:750	the tensile strength	731:750	And, the tensile strength and elongation at break of the NPCS-CA/PVA (5/5) film with 0.4 % CEO were 20.32 MPa and 65.73 %, respectively.					
36870659	4	48	from	break	770:774	arg1	20.32 MPa					826:834	20.32 MPa	826:834	20.32 MPa	826:834	And, the tensile strength and elongation at break of the NPCS-CA/PVA (5/5) film with 0.4 % CEO were 20.32 MPa and 65.73 %, respectively.					
36870659	4	48	from	break	770:774	arg1	elongation					756:765	elongation	756:765	elongation	756:765	And, the tensile strength and elongation at break of the NPCS-CA/PVA (5/5) film with 0.4 % CEO were 20.32 MPa and 65.73 %, respectively.					
36870659	0	49	theme	alcohol	70:76	arg1	films					88:92	functionalized chitosan/polyvinyl alcohol composite films	36:92	functionalized chitosan/polyvinyl alcohol composite films	36:92	Preparation and characterization of functionalized chitosan/polyvinyl alcohol composite films incorporated with cinnamon essential oil as an active packaging material.					
36870659	2	50	theme	1H	547:548	arg1	NMR					550:552	1H NMR	547:552	1H NMR	547:552	The chemical structure of the chitosan derivative was characterized by FT-IR, 1H NMR and XRD.					
36870659	8	51	theme	NPCS-CA/PVA-CEO	1438:1452	arg1	material					1510:1517	biocomposite food packaging material	1482:1517	biocomposite food packaging material	1482:1517	The NPCS-CA/PVA-CEO films could be developed as biocomposite food packaging material.					
36870659	8	51	theme	NPCS-CA/PVA-CEO	1438:1452	arg1	films					1454:1458	The NPCS-CA/PVA-CEO films	1434:1458	The NPCS-CA/PVA-CEO films	1434:1458	The NPCS-CA/PVA-CEO films could be developed as biocomposite food packaging material.					
36870659	6	52	theme	NPCS-CA/PVA	1253:1263	arg1	proportion					1265:1274	NPCS-CA/PVA proportion	1253:1274	NPCS-CA/PVA proportion	1253:1274	Furthermore, the antibacterial property of film-forming solutions against E. coli, S. aureus, and C. lagenarium was distinctly improved with the increase of NPCS-CA/PVA proportion.					
36870659	5	53	theme	composite	909:917	arg1	films					919:923	the NPCS-CA/PVA-CEO composite films	889:923	the NPCS-CA/PVA-CEO composite films	889:923	The results revealed that the NPCS-CA/PVA-CEO composite films exhibited an excellent ultraviolet barrier property at 200-300 nm and significantly reduced oxygen permeability, carbon dioxide permeability and water vapor permeability.					
36870659	4	54	theme	tensile	735:741	arg1	strength					743:750	the tensile strength	731:750	the tensile strength	731:750	And, the tensile strength and elongation at break of the NPCS-CA/PVA (5/5) film with 0.4 % CEO were 20.32 MPa and 65.73 %, respectively.					
36870659	4	54	theme	tensile	735:741	arg1	20.32 MPa					826:834	20.32 MPa	826:834	20.32 MPa	826:834	And, the tensile strength and elongation at break of the NPCS-CA/PVA (5/5) film with 0.4 % CEO were 20.32 MPa and 65.73 %, respectively.					
36870659	8	55	theme	food	1495:1498	arg1	films					1454:1458	The NPCS-CA/PVA-CEO films	1434:1458	The NPCS-CA/PVA-CEO films	1434:1458	The NPCS-CA/PVA-CEO films could be developed as biocomposite food packaging material.					
36870659	8	55	theme	food	1495:1498	arg1	material					1510:1517	biocomposite food packaging material	1482:1517	biocomposite food packaging material	1482:1517	The NPCS-CA/PVA-CEO films could be developed as biocomposite food packaging material.					
36870659	3	56	theme	FT-IR	595:599	arg1	characterization					575:590	the characterization	571:590	the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films	571:662	Through the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films, the optimal proportion of NPCS-CA/PVA was determined as 5/5.					
36870659	4	57	with	film	801:804	arg1	CEO					817:819	0.4 % CEO	811:819	0.4 % CEO	811:819	And, the tensile strength and elongation at break of the NPCS-CA/PVA (5/5) film with 0.4 % CEO were 20.32 MPa and 65.73 %, respectively.					
36870659	1	58	theme	quaternary	242:251	arg1	salt					265:268	quaternary phosphonium salt	242:268	quaternary phosphonium salt	242:268	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	2	59	theme	chemical	473:480	arg1	structure					482:490	The chemical structure	469:490	The chemical structure of the chitosan derivative	469:517	The chemical structure of the chitosan derivative was characterized by FT-IR, 1H NMR and XRD.					
36870659	1	60	theme	cinnamon	417:424	arg1	CEO					441:443	CEO	441:443	CEO	441:443	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	1	60	theme	cinnamon	417:424	arg1	oil					436:438	cinnamon essential oil	417:438	cinnamon essential oil (CEO)	417:444	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	1	61	theme	phosphonium	253:263	arg1	salt					265:268	quaternary phosphonium salt	242:268	quaternary phosphonium salt	242:268	In this study, amphiphilic chitosan (NPCS-CA) was synthesized by grafting quaternary phosphonium salt and cholic acid onto the chain of chitosan, aiming to develop an active edible film based on NPCS-CA and polyvinyl alcohol (PVA) incorporated with cinnamon essential oil (CEO) by the casting method.					
36870659	0	62	theme	films	88:92	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of functionalized chitosan/polyvinyl alcohol composite films incorporated with cinnamon essential oil as an active packaging material.					
36870659	0	62	theme	films	88:92	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of functionalized chitosan/polyvinyl alcohol composite films incorporated with cinnamon essential oil as an active packaging material.					
36870659	3	63	theme	films	658:662	arg1	properties					630:639	mechanical and barrier properties	607:639	properties	630:639	Through the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films, the optimal proportion of NPCS-CA/PVA was determined as 5/5.					
36870659	3	63	theme	films	658:662	arg1	FT-IR					595:599	FT-IR	595:599	FT-IR	595:599	Through the characterization of FT-IR, TGA, mechanical and barrier properties of the composite films, the optimal proportion of NPCS-CA/PVA was determined as 5/5.					
35862452	8	0	theme	strains	1608:1614	arg1	bacteriophages					1573:1586	temperate bacteriophages	1563:1586	temperate bacteriophages of chitin-degrading strains	1563:1614	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	8	0	theme	strains	1608:1614	arg1	members					1630:1636	the keystone members	1617:1636	the keystone members of the community	1617:1653	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	6	1	from	populations	1114:1124	arg1	subset					1131:1136	a subset	1129:1136	a subset of particles	1129:1149	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	6	1	from	populations	1114:1124	arg1	rare					1079:1082	rare	1079:1082	rare	1079:1082	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	5	2	from	variation	934:942	arg1	composition					968:978	microscale community composition	947:978	microscale community composition	947:978	Using low-template shotgun metagenomics and imaging, we find significant variation in microscale community composition despite the similarity in initial species pools across replicates.					
35862452	6	3	theme	established	1096:1106	arg1	populations					1114:1124	seawater established large populations	1087:1124	seawater established large populations on a subset of particles	1087:1149	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	8	4	theme	keystone	1621:1628	arg1	bacteriophages					1573:1586	temperate bacteriophages	1563:1586	temperate bacteriophages of chitin-degrading strains	1563:1614	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	8	4	theme	keystone	1621:1628	arg1	members					1630:1636	the keystone members	1617:1636	the keystone members of the community	1617:1653	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	2	5	theme	microscale	362:371	arg1	ecosystems					373:382	such microscale ecosystems	357:382	such microscale ecosystems	357:382	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	6	6	theme	seawater	1087:1094	arg1	populations					1114:1124	seawater established large populations	1087:1124	seawater established large populations on a subset of particles	1087:1149	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	2	7	from	growth	222:227	arg1	patches					275:281	these resource patches	260:281	these resource patches	260:281	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	7	8	theme	growth	1422:1427	arg1	tempo					1413:1417	the tempo	1409:1417	the tempo of growth on particles	1409:1440	We show, through a mathematical model, that this variability can be attributed to stochastic colonization and historical contingencies affecting the tempo of growth on particles.					
35862452	3	9	theme	natural	628:634	arg1	populations					636:646	natural populations	628:646	natural populations	628:646	Despite the potential importance of patch-level dynamics for the large-scale functioning of heterotrophic microbial communities, we have not yet been able to delineate the ecological processes that control natural populations at the microscale.					
35862452	7	10	theme	mathematical	1283:1294	arg1	model					1296:1300	a mathematical model	1281:1300	a mathematical model	1281:1300	We show, through a mathematical model, that this variability can be attributed to stochastic colonization and historical contingencies affecting the tempo of growth on particles.					
35862452	5	11	theme	shotgun	880:886	arg1	metagenomics					888:899	low-template shotgun metagenomics	867:899	low-template shotgun metagenomics	867:899	Using low-template shotgun metagenomics and imaging, we find significant variation in microscale community composition despite the similarity in initial species pools across replicates.					
35862452	9	12	theme	significant	1780:1790	arg1	consequences					1792:1803	significant consequences	1780:1803	significant consequences for the diversity and functionality of systems of microscale patches	1780:1872	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	8	13	theme	biological	1469:1478	arg1	process					1480:1486	one biological process	1465:1486	one biological process leading to such noisy growth across particles	1465:1532	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	1	14	theme	microscale	158:167	arg1	patches					178:184	microscale resource patches	158:184	microscale resource patches made of complex organic matter	158:215	In many natural environments, microorganisms decompose microscale resource patches made of complex organic matter.					
35862452	2	15	from	collapse	233:240	arg1	patches					275:281	these resource patches	260:281	these resource patches	260:281	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	6	16	theme	particles	1141:1149	arg1	subset					1131:1136	a subset	1129:1136	a subset of particles	1129:1149	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	4	17	theme	abundant	829:836	arg1	chitin					812:817	chitin	812:817	chitin	812:817	Here, we address this challenge by characterizing the natural marine communities that assembled on over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide.					
35862452	4	17	theme	abundant	829:836	arg1	polysaccharide					845:858	the most abundant marine polysaccharide	820:858	the most abundant marine polysaccharide	820:858	Here, we address this challenge by characterizing the natural marine communities that assembled on over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide.					
35862452	2	18	theme	metabolism	410:419	arg1	hotspots					384:391	hotspots	384:391	hotspots of heterotrophic metabolism	384:419	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	1	19	theme	complex	194:200	arg1	matter					210:215	complex organic matter	194:215	complex organic matter	194:215	In many natural environments, microorganisms decompose microscale resource patches made of complex organic matter.					
35862452	3	20	theme	potential	434:442	arg1	importance					444:453	the potential importance	430:453	the potential importance of patch-level dynamics for the large-scale functioning of heterotrophic microbial communities	430:548	Despite the potential importance of patch-level dynamics for the large-scale functioning of heterotrophic microbial communities, we have not yet been able to delineate the ecological processes that control natural populations at the microscale.					
35862452	8	21	theme	community	1645:1653	arg1	bacteriophages					1573:1586	temperate bacteriophages	1563:1586	temperate bacteriophages of chitin-degrading strains	1563:1614	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	8	21	theme	community	1645:1653	arg1	members					1630:1636	the keystone members	1617:1636	the keystone members of the community	1617:1653	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	6	22	from	rare	1079:1082	arg1	populations					1114:1124	seawater established large populations	1087:1124	seawater established large populations on a subset of particles	1087:1149	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	2	23	theme	spatial	297:303	arg1	ranges					305:310	spatial ranges	297:310	spatial ranges of a few hundred micrometers or less	297:347	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	1	24	theme	organic	202:208	arg1	matter					210:215	complex organic matter	194:215	complex organic matter	194:215	In many natural environments, microorganisms decompose microscale resource patches made of complex organic matter.					
35862452	5	25	theme	low-template	867:878	arg1	metagenomics					888:899	low-template shotgun metagenomics	867:899	low-template shotgun metagenomics	867:899	Using low-template shotgun metagenomics and imaging, we find significant variation in microscale community composition despite the similarity in initial species pools across replicates.					
35862452	6	26	theme	Chitin-degrading	1047:1062	arg1	taxa					1064:1067	Chitin-degrading taxa	1047:1067	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.	1047:1262	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	6	27	theme	wide	1167:1170	arg1	range					1172:1176	a wide range	1165:1176	a wide range of predicted chitinolytic abilities and biomass	1165:1224	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	9	28	theme	initial	1662:1668	arg1	stochasticity					1670:1682	initial stochasticity	1662:1682	initial stochasticity in assembly states on individual particles, amplified through ecological interactions,	1662:1769	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	6	29	theme	individual	1242:1251	arg1	particles					1253:1261	individual particles	1242:1261	individual particles	1242:1261	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	7	30	theme	stochastic	1346:1355	arg1	colonization					1357:1368	stochastic colonization	1346:1368	stochastic colonization	1346:1368	We show, through a mathematical model, that this variability can be attributed to stochastic colonization and historical contingencies affecting the tempo of growth on particles.					
35862452	3	31	theme	patch-level	458:468	arg1	dynamics					470:477	patch-level dynamics	458:477	patch-level dynamics	458:477	Despite the potential importance of patch-level dynamics for the large-scale functioning of heterotrophic microbial communities, we have not yet been able to delineate the ecological processes that control natural populations at the microscale.					
35862452	0	32	theme	Historical	0:9	arg1	contingencies					11:23	Historical contingencies	0:23	Historical contingencies	0:23	Historical contingencies and phage induction diversify bacterioplankton communities at the microscale.					
35862452	9	33	theme	assembly	1687:1694	arg1	states					1696:1701	assembly states	1687:1701	assembly states	1687:1701	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	5	34	from	similarity	992:1001	arg1	pools					1022:1026	initial species pools	1006:1026	initial species pools	1006:1026	Using low-template shotgun metagenomics and imaging, we find significant variation in microscale community composition despite the similarity in initial species pools across replicates.					
35862452	1	35	theme	many	106:109	arg1	environments					119:130	many natural environments	106:130	many natural environments	106:130	In many natural environments, microorganisms decompose microscale resource patches made of complex organic matter.					
35862452	9	36	contain	have	1775:1778	arg2	consequences					1792:1803	significant consequences	1780:1803	significant consequences for the diversity and functionality of systems of microscale patches	1780:1872	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	9	36	contain	have	1775:1778	arg1	stochasticity					1670:1682	initial stochasticity	1662:1682	initial stochasticity in assembly states on individual particles, amplified through ecological interactions,	1662:1769	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	2	37	theme	heterotrophic	396:408	arg1	metabolism					410:419	heterotrophic metabolism	396:419	heterotrophic metabolism	396:419	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	0	38	theme	phage	29:33	arg1	induction					35:43	phage induction	29:43	phage induction	29:43	Historical contingencies and phage induction diversify bacterioplankton communities at the microscale.					
35862452	4	39	theme	microscale	788:797	arg1	particles					799:807	over 1,000 individual microscale particles	766:807	over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide	766:858	Here, we address this challenge by characterizing the natural marine communities that assembled on over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide.					
35862452	5	40	theme	species	1014:1020	arg1	pools					1022:1026	initial species pools	1006:1026	initial species pools	1006:1026	Using low-template shotgun metagenomics and imaging, we find significant variation in microscale community composition despite the similarity in initial species pools across replicates.					
35862452	1	41	theme	natural	111:117	arg1	environments					119:130	many natural environments	106:130	many natural environments	106:130	In many natural environments, microorganisms decompose microscale resource patches made of complex organic matter.					
35862452	2	42	theme	micrometers	329:339	arg1	ranges					305:310	spatial ranges	297:310	spatial ranges of a few hundred micrometers or less	297:347	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	8	43	theme	differential	1537:1548	arg1	predation					1550:1558	differential predation	1537:1558	differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community	1537:1653	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	2	44	theme	few	317:319	arg1	micrometers					329:339	a few hundred micrometers	315:339	a few hundred micrometers	315:339	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	3	45	theme	heterotrophic	514:526	arg1	communities					538:548	heterotrophic microbial communities	514:548	heterotrophic microbial communities	514:548	Despite the potential importance of patch-level dynamics for the large-scale functioning of heterotrophic microbial communities, we have not yet been able to delineate the ecological processes that control natural populations at the microscale.					
35862452	4	46	theme	natural	721:727	arg1	communities					736:746	the natural marine communities	717:746	the natural marine communities that assembled on over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide	717:858	Here, we address this challenge by characterizing the natural marine communities that assembled on over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide.					
35862452	6	47	theme	biomass	1218:1224	arg1	range					1172:1176	a wide range	1165:1176	a wide range of predicted chitinolytic abilities and biomass	1165:1224	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	3	48	theme	dynamics	470:477	arg1	importance					444:453	the potential importance	430:453	the potential importance of patch-level dynamics for the large-scale functioning of heterotrophic microbial communities	430:548	Despite the potential importance of patch-level dynamics for the large-scale functioning of heterotrophic microbial communities, we have not yet been able to delineate the ecological processes that control natural populations at the microscale.					
35862452	7	49	theme	historical	1374:1383	arg1	contingencies					1385:1397	historical contingencies	1374:1397	historical contingencies affecting the tempo of growth on particles	1374:1440	We show, through a mathematical model, that this variability can be attributed to stochastic colonization and historical contingencies affecting the tempo of growth on particles.					
35862452	5	50	theme	significant	922:932	arg1	variation					934:942	significant variation	922:942	significant variation in microscale community composition	922:978	Using low-template shotgun metagenomics and imaging, we find significant variation in microscale community composition despite the similarity in initial species pools across replicates.					
35862452	9	51	theme	systems	1844:1850	arg1	functionality					1827:1839	functionality	1827:1839	functionality	1827:1839	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	9	51	theme	systems	1844:1850	arg1	diversity					1813:1821	diversity	1813:1821	diversity	1813:1821	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	9	52	theme	individual	1706:1715	arg1	particles					1717:1725	individual particles	1706:1725	individual particles	1706:1725	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	4	53	theme	chitin	812:817	arg1	particles					799:807	over 1,000 individual microscale particles	766:807	over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide	766:858	Here, we address this challenge by characterizing the natural marine communities that assembled on over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide.					
35862452	5	54	theme	community	958:966	arg1	composition					968:978	microscale community composition	947:978	microscale community composition	947:978	Using low-template shotgun metagenomics and imaging, we find significant variation in microscale community composition despite the similarity in initial species pools across replicates.					
35862452	2	55	theme	populations	245:255	arg1	growth					222:227	growth	222:227	growth	222:227	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	2	55	theme	populations	245:255	arg1	collapse					233:240	collapse	233:240	collapse	233:240	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	6	56	theme	chitinolytic	1191:1202	arg1	abilities					1204:1212	predicted chitinolytic abilities	1181:1212	predicted chitinolytic abilities	1181:1212	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	2	57	theme	such	357:360	arg1	ecosystems					373:382	such microscale ecosystems	357:382	such microscale ecosystems	357:382	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	0	58	theme	bacterioplankton	55:70	arg1	communities					72:82	bacterioplankton communities	55:82	bacterioplankton communities	55:82	Historical contingencies and phage induction diversify bacterioplankton communities at the microscale.					
35862452	8	59	theme	such	1499:1502	arg1	growth					1510:1515	such noisy growth	1499:1515	such noisy growth across particles	1499:1532	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	9	60	theme	microscale	1855:1864	arg1	patches					1866:1872	microscale patches	1855:1872	microscale patches	1855:1872	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	8	61	theme	temperate	1563:1571	arg1	bacteriophages					1573:1586	temperate bacteriophages	1563:1586	temperate bacteriophages of chitin-degrading strains	1563:1614	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	8	61	theme	temperate	1563:1571	arg1	members					1630:1636	the keystone members	1617:1636	the keystone members of the community	1617:1653	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	5	62	theme	initial	1006:1012	arg1	pools					1022:1026	initial species pools	1006:1026	initial species pools	1006:1026	Using low-template shotgun metagenomics and imaging, we find significant variation in microscale community composition despite the similarity in initial species pools across replicates.					
35862452	9	63	dep	diversity	1813:1821	arg1	the					1809:1811	the	1809:1811	the	1809:1811	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	9	64	theme	patches	1866:1872	arg1	systems					1844:1850	systems	1844:1850	systems of microscale patches	1844:1872	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	5	65	theme	microscale	947:956	arg1	composition					968:978	microscale community composition	947:978	microscale community composition	947:978	Using low-template shotgun metagenomics and imaging, we find significant variation in microscale community composition despite the similarity in initial species pools across replicates.					
35862452	3	66	theme	microbial	528:536	arg1	communities					538:548	heterotrophic microbial communities	514:548	heterotrophic microbial communities	514:548	Despite the potential importance of patch-level dynamics for the large-scale functioning of heterotrophic microbial communities, we have not yet been able to delineate the ecological processes that control natural populations at the microscale.					
35862452	3	67	theme	ecological	594:603	arg1	processes					605:613	the ecological processes	590:613	the ecological processes that control natural populations at the microscale	590:664	Despite the potential importance of patch-level dynamics for the large-scale functioning of heterotrophic microbial communities, we have not yet been able to delineate the ecological processes that control natural populations at the microscale.					
35862452	6	68	theme	abilities	1204:1212	arg1	range					1172:1176	a wide range	1165:1176	a wide range of predicted chitinolytic abilities and biomass	1165:1224	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	8	69	theme	chitin-degrading	1591:1606	arg1	strains					1608:1614	chitin-degrading strains	1591:1614	chitin-degrading strains	1591:1614	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	4	70	theme	marine	838:843	arg1	chitin					812:817	chitin	812:817	chitin	812:817	Here, we address this challenge by characterizing the natural marine communities that assembled on over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide.					
35862452	4	70	theme	marine	838:843	arg1	polysaccharide					845:858	the most abundant marine polysaccharide	820:858	the most abundant marine polysaccharide	820:858	Here, we address this challenge by characterizing the natural marine communities that assembled on over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide.					
35862452	7	71	from	tempo	1413:1417	arg1	particles					1432:1440	particles	1432:1440	particles	1432:1440	We show, through a mathematical model, that this variability can be attributed to stochastic colonization and historical contingencies affecting the tempo of growth on particles.					
35862452	2	72	theme	less	344:347	arg1	ranges					305:310	spatial ranges	297:310	spatial ranges of a few hundred micrometers or less	297:347	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	9	73	from	stochasticity	1670:1682	arg1	particles					1717:1725	individual particles	1706:1725	individual particles	1706:1725	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	9	73	from	stochasticity	1670:1682	arg1	states					1696:1701	assembly states	1687:1701	assembly states	1687:1701	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	4	74	theme	individual	777:786	arg1	particles					799:807	over 1,000 individual microscale particles	766:807	over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide	766:858	Here, we address this challenge by characterizing the natural marine communities that assembled on over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide.					
35862452	8	75	theme	noisy	1504:1508	arg1	growth					1510:1515	such noisy growth	1499:1515	such noisy growth across particles	1499:1532	We find evidence that one biological process leading to such noisy growth across particles is differential predation by temperate bacteriophages of chitin-degrading strains, the keystone members of the community.					
35862452	6	76	theme	predicted	1181:1189	arg1	abilities					1204:1212	predicted chitinolytic abilities	1181:1212	predicted chitinolytic abilities	1181:1212	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	9	77	theme	ecological	1746:1755	arg1	interactions					1757:1768	ecological interactions	1746:1768	ecological interactions	1746:1768	Thus, initial stochasticity in assembly states on individual particles, amplified through ecological interactions, may have significant consequences for the diversity and functionality of systems of microscale patches.					
35862452	6	78	theme	particles	1253:1261	arg1	level					1233:1237	the level	1229:1237	the level of individual particles	1229:1261	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
35862452	1	79	theme	resource	169:176	arg1	patches					178:184	microscale resource patches	158:184	microscale resource patches made of complex organic matter	158:215	In many natural environments, microorganisms decompose microscale resource patches made of complex organic matter.					
35862452	3	80	theme	communities	538:548	arg1	large-scale					487:497	large-scale	487:497	large-scale	487:497	Despite the potential importance of patch-level dynamics for the large-scale functioning of heterotrophic microbial communities, we have not yet been able to delineate the ecological processes that control natural populations at the microscale.					
35862452	2	81	theme	resource	266:273	arg1	patches					275:281	these resource patches	260:281	these resource patches	260:281	The growth and collapse of populations on these resource patches unfold within spatial ranges of a few hundred micrometers or less, making such microscale ecosystems hotspots of heterotrophic metabolism.					
35862452	4	82	theme	marine	729:734	arg1	communities					736:746	the natural marine communities	717:746	the natural marine communities that assembled on over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide	717:858	Here, we address this challenge by characterizing the natural marine communities that assembled on over 1,000 individual microscale particles of chitin, the most abundant marine polysaccharide.					
35862452	6	83	theme	large	1108:1112	arg1	populations					1114:1124	seawater established large populations	1087:1124	seawater established large populations on a subset of particles	1087:1149	Chitin-degrading taxa that were rare in seawater established large populations on a subset of particles, resulting in a wide range of predicted chitinolytic abilities and biomass at the level of individual particles.					
36604080	7	0	theme	Salecan	1260:1266	arg1	effect					1250:1255	the hepatoprotective and anti-inflammatory effect	1207:1255	the hepatoprotective and anti-inflammatory effect of Salecan against liver injury	1207:1287	Besides, microbiota-derived metabolites of Salecan also contributed to the hepatoprotective and anti-inflammatory effect of Salecan against liver injury.					
36604080	6	1	theme	microbiota	987:996	arg1	transplantation					998:1012	Fecal microbiota transplantation	981:1012	Fecal microbiota transplantation	981:1012	Fecal microbiota transplantation was used to further verify the mechanism, and we confirmed that microbial colonization partially alleviated liver injury.					
36604080	8	2	theme	gut	1379:1381	arg1	microbiota					1383:1392	gut microbiota	1379:1392	gut microbiota	1379:1392	These findings supported that Salecan intervention attenuated liver injury by regulating gut microbiota and its metabolites.					
36604080	7	3	theme	microbiota-derived	1145:1162	arg1	metabolites					1164:1174	microbiota-derived metabolites	1145:1174	microbiota-derived metabolites of Salecan	1145:1185	Besides, microbiota-derived metabolites of Salecan also contributed to the hepatoprotective and anti-inflammatory effect of Salecan against liver injury.					
36604080	6	4	theme	liver	1122:1126	arg1	injury					1128:1133	liver injury	1122:1133	liver injury	1122:1133	Fecal microbiota transplantation was used to further verify the mechanism, and we confirmed that microbial colonization partially alleviated liver injury.					
36604080	1	5	theme	positive	222:229	arg1	effects					238:244	positive health effects	222:244	positive health effects	222:244	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	5	6	theme	gut	965:967	arg1	microbiota					969:978	the gut microbiota	961:978	the gut microbiota	961:978	In addition, Salecan treatment modulated the composition of gut microbiota and antibiotic cocktail treatment indicated that the hepatoprotective effect of Salecan was dependent on the gut microbiota.					
36604080	1	7	theme	health	231:236	arg1	effects					238:244	positive health effects	222:244	positive health effects	222:244	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	3	8	from	injury	610:615	arg1	mechanism					551:559	the underlying mechanism	536:559	the underlying mechanism of Salecan supplementation in CCl4-induced liver injury	536:615	Here, the anti-inflammatory effects and the underlying mechanism of Salecan supplementation in CCl4-induced liver injury were investigated.					
36604080	3	8	from	injury	610:615	arg1	effects					524:530	the anti-inflammatory effects	502:530	the anti-inflammatory effects	502:530	Here, the anti-inflammatory effects and the underlying mechanism of Salecan supplementation in CCl4-induced liver injury were investigated.					
36604080	6	9	used	used	1018:1021	arg2	transplantation					998:1012	Fecal microbiota transplantation	981:1012	Fecal microbiota transplantation	981:1012	Fecal microbiota transplantation was used to further verify the mechanism, and we confirmed that microbial colonization partially alleviated liver injury.					
36604080	1	10	theme	effects	238:244	arg1	variety					211:217	a variety	209:217	a variety of positive health effects	209:244	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	1	10	theme	effects	238:244	arg1	effects					238:244	positive health effects	222:244	positive health effects	222:244	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	2	11	theme	health	465:470	arg1	effects					472:478	their positive health effects	450:478	their positive health effects remain elusive	450:493	In addition, β-glucan could induce the shifts in gut microbiota, however, whether modulation of gut microbiota by β-glucan is associated with their positive health effects remain elusive.					
36604080	4	12	theme	macrophage	756:765	arg1	polarization					767:778	M2 macrophage polarization	753:778	M2 macrophage polarization	753:778	After 8 weeks of treatment, we observed that Salecan alleviated liver injury by regulating inflammatory response and M2 macrophage polarization.					
36604080	3	13	from	supplementation	572:586	arg1	injury					610:615	CCl4-induced liver injury	591:615	CCl4-induced liver injury	591:615	Here, the anti-inflammatory effects and the underlying mechanism of Salecan supplementation in CCl4-induced liver injury were investigated.					
36604080	1	14	theme	novel	152:156	arg1	ingredients					163:173	the novel food ingredients	148:173	the novel food ingredients approved in China	148:191	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	2	15	theme	positive	456:463	arg1	effects					472:478	their positive health effects	450:478	their positive health effects remain elusive	450:493	In addition, β-glucan could induce the shifts in gut microbiota, however, whether modulation of gut microbiota by β-glucan is associated with their positive health effects remain elusive.					
36604080	7	16	theme	Salecan	1179:1185	arg1	metabolites					1164:1174	microbiota-derived metabolites	1145:1174	microbiota-derived metabolites of Salecan	1145:1185	Besides, microbiota-derived metabolites of Salecan also contributed to the hepatoprotective and anti-inflammatory effect of Salecan against liver injury.					
36604080	4	17	theme	M2	753:754	arg1	polarization					767:778	M2 macrophage polarization	753:778	M2 macrophage polarization	753:778	After 8 weeks of treatment, we observed that Salecan alleviated liver injury by regulating inflammatory response and M2 macrophage polarization.					
36604080	1	18	theme	food	158:161	arg1	ingredients					163:173	the novel food ingredients	148:173	the novel food ingredients approved in China	148:191	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	7	19	theme	liver	1276:1280	arg1	injury					1282:1287	liver injury	1276:1287	liver injury	1276:1287	Besides, microbiota-derived metabolites of Salecan also contributed to the hepatoprotective and anti-inflammatory effect of Salecan against liver injury.					
36604080	1	20	theme	ingredients	163:173	arg1	ingredients					163:173	the novel food ingredients	148:173	the novel food ingredients approved in China	148:191	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	1	20	theme	ingredients	163:173	arg1	one					141:143	one	141:143	one	141:143	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	1	20	theme	ingredients	163:173	arg1	Salecan					109:115	Salecan	109:115	Salecan	109:115	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	5	21	theme	Salecan	794:800	arg1	treatment					802:810	Salecan treatment	794:810	Salecan treatment	794:810	In addition, Salecan treatment modulated the composition of gut microbiota and antibiotic cocktail treatment indicated that the hepatoprotective effect of Salecan was dependent on the gut microbiota.					
36604080	6	22	theme	Fecal	981:985	arg1	transplantation					998:1012	Fecal microbiota transplantation	981:1012	Fecal microbiota transplantation	981:1012	Fecal microbiota transplantation was used to further verify the mechanism, and we confirmed that microbial colonization partially alleviated liver injury.					
36604080	1	23	theme	approved	175:182	arg1	ingredients					163:173	the novel food ingredients	148:173	the novel food ingredients approved in China	148:191	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	4	24	theme	inflammatory	727:738	arg1	response					740:747	inflammatory response	727:747	inflammatory response	727:747	After 8 weeks of treatment, we observed that Salecan alleviated liver injury by regulating inflammatory response and M2 macrophage polarization.					
36604080	8	25	theme	Salecan	1320:1326	arg1	intervention					1328:1339	Salecan intervention	1320:1339	Salecan intervention	1320:1339	These findings supported that Salecan intervention attenuated liver injury by regulating gut microbiota and its metabolites.					
36604080	0	26	theme	anti-inflammatory	16:32	arg1	effects					34:40	anti-inflammatory effects	16:40	anti-inflammatory effects	16:40	Salecan confers anti-inflammatory effects in liver injury via regulating gut microbiota and its metabolites.					
36604080	3	27	theme	anti-inflammatory	506:522	arg1	effects					524:530	the anti-inflammatory effects	502:530	the anti-inflammatory effects	502:530	Here, the anti-inflammatory effects and the underlying mechanism of Salecan supplementation in CCl4-induced liver injury were investigated.					
36604080	3	28	from	mechanism	551:559	arg1	injury					610:615	CCl4-induced liver injury	591:615	CCl4-induced liver injury	591:615	Here, the anti-inflammatory effects and the underlying mechanism of Salecan supplementation in CCl4-induced liver injury were investigated.					
36604080	0	29	theme	liver	45:49	arg1	injury					51:56	liver injury	45:56	liver injury	45:56	Salecan confers anti-inflammatory effects in liver injury via regulating gut microbiota and its metabolites.					
36604080	7	30	theme	hepatoprotective	1211:1226	arg1	effect					1250:1255	the hepatoprotective and anti-inflammatory effect	1207:1255	the hepatoprotective and anti-inflammatory effect of Salecan against liver injury	1207:1287	Besides, microbiota-derived metabolites of Salecan also contributed to the hepatoprotective and anti-inflammatory effect of Salecan against liver injury.					
36604080	5	31	theme	cocktail	871:878	arg1	treatment					880:888	antibiotic cocktail treatment	860:888	antibiotic cocktail treatment	860:888	In addition, Salecan treatment modulated the composition of gut microbiota and antibiotic cocktail treatment indicated that the hepatoprotective effect of Salecan was dependent on the gut microbiota.					
36604080	5	32	theme	antibiotic	860:869	arg1	treatment					880:888	antibiotic cocktail treatment	860:888	antibiotic cocktail treatment	860:888	In addition, Salecan treatment modulated the composition of gut microbiota and antibiotic cocktail treatment indicated that the hepatoprotective effect of Salecan was dependent on the gut microbiota.					
36604080	5	33	theme	microbiota	845:854	arg1	composition					826:836	the composition	822:836	the composition of gut microbiota	822:854	In addition, Salecan treatment modulated the composition of gut microbiota and antibiotic cocktail treatment indicated that the hepatoprotective effect of Salecan was dependent on the gut microbiota.					
36604080	2	34	dep	effects	472:478	arg1	remain					480:485	remain	480:485	remain elusive	480:493	In addition, β-glucan could induce the shifts in gut microbiota, however, whether modulation of gut microbiota by β-glucan is associated with their positive health effects remain elusive.					
36604080	3	35	theme	liver	604:608	arg1	injury					610:615	CCl4-induced liver injury	591:615	CCl4-induced liver injury	591:615	Here, the anti-inflammatory effects and the underlying mechanism of Salecan supplementation in CCl4-induced liver injury were investigated.					
36604080	2	36	theme	gut	357:359	arg1	microbiota					361:370	gut microbiota	357:370	gut microbiota	357:370	In addition, β-glucan could induce the shifts in gut microbiota, however, whether modulation of gut microbiota by β-glucan is associated with their positive health effects remain elusive.					
36604080	2	37	theme	microbiota	408:417	arg1	modulation					390:399	modulation	390:399	modulation of gut microbiota by β-glucan	390:429	In addition, β-glucan could induce the shifts in gut microbiota, however, whether modulation of gut microbiota by β-glucan is associated with their positive health effects remain elusive.					
36604080	1	38	theme	liver	268:272	arg1	injury					274:279	liver injury	268:279	liver injury	268:279	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	5	39	theme	hepatoprotective	909:924	arg1	effect					926:931	the hepatoprotective effect	905:931	the hepatoprotective effect of Salecan	905:942	In addition, Salecan treatment modulated the composition of gut microbiota and antibiotic cocktail treatment indicated that the hepatoprotective effect of Salecan was dependent on the gut microbiota.					
36604080	5	39	theme	hepatoprotective	909:924	arg1	dependent					948:956	dependent	948:956	dependent	948:956	In addition, Salecan treatment modulated the composition of gut microbiota and antibiotic cocktail treatment indicated that the hepatoprotective effect of Salecan was dependent on the gut microbiota.					
36604080	2	40	theme	gut	404:406	arg1	microbiota					408:417	gut microbiota	404:417	gut microbiota	404:417	In addition, β-glucan could induce the shifts in gut microbiota, however, whether modulation of gut microbiota by β-glucan is associated with their positive health effects remain elusive.					
36604080	1	41	theme	injury	274:279	arg1	mechanism					255:263	the mechanism	251:263	the mechanism of liver injury	251:279	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	7	42	theme	anti-inflammatory	1232:1248	arg1	effect					1250:1255	the hepatoprotective and anti-inflammatory effect	1207:1255	the hepatoprotective and anti-inflammatory effect of Salecan against liver injury	1207:1287	Besides, microbiota-derived metabolites of Salecan also contributed to the hepatoprotective and anti-inflammatory effect of Salecan against liver injury.					
36604080	3	43	theme	underlying	540:549	arg1	mechanism					551:559	the underlying mechanism	536:559	the underlying mechanism of Salecan supplementation in CCl4-induced liver injury	536:615	Here, the anti-inflammatory effects and the underlying mechanism of Salecan supplementation in CCl4-induced liver injury were investigated.					
36604080	8	44	theme	liver	1352:1356	arg1	injury					1358:1363	liver injury	1352:1363	liver injury	1352:1363	These findings supported that Salecan intervention attenuated liver injury by regulating gut microbiota and its metabolites.					
36604080	1	45	from	China	187:191	arg1	approved					175:182	approved	175:182	approved	175:182	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	1	46	theme	natural	120:126	arg1	β-glucan					128:135	a natural β-glucan	118:135	a natural β-glucan	118:135	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	1	46	theme	natural	120:126	arg1	Salecan					109:115	Salecan	109:115	Salecan	109:115	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	2	47	from	shifts	347:352	arg1	microbiota					361:370	gut microbiota	357:370	gut microbiota	357:370	In addition, β-glucan could induce the shifts in gut microbiota, however, whether modulation of gut microbiota by β-glucan is associated with their positive health effects remain elusive.					
36604080	3	48	theme	CCl4-induced	591:602	arg1	injury					610:615	CCl4-induced liver injury	591:615	CCl4-induced liver injury	591:615	Here, the anti-inflammatory effects and the underlying mechanism of Salecan supplementation in CCl4-induced liver injury were investigated.					
36604080	5	49	theme	Salecan	936:942	arg1	effect					926:931	the hepatoprotective effect	905:931	the hepatoprotective effect of Salecan	905:942	In addition, Salecan treatment modulated the composition of gut microbiota and antibiotic cocktail treatment indicated that the hepatoprotective effect of Salecan was dependent on the gut microbiota.					
36604080	5	49	theme	Salecan	936:942	arg1	dependent					948:956	dependent	948:956	dependent	948:956	In addition, Salecan treatment modulated the composition of gut microbiota and antibiotic cocktail treatment indicated that the hepatoprotective effect of Salecan was dependent on the gut microbiota.					
36604080	3	50	from	effects	524:530	arg1	injury					610:615	CCl4-induced liver injury	591:615	CCl4-induced liver injury	591:615	Here, the anti-inflammatory effects and the underlying mechanism of Salecan supplementation in CCl4-induced liver injury were investigated.					
36604080	4	51	theme	liver	700:704	arg1	injury					706:711	liver injury	700:711	liver injury	700:711	After 8 weeks of treatment, we observed that Salecan alleviated liver injury by regulating inflammatory response and M2 macrophage polarization.					
36604080	0	52	theme	gut	73:75	arg1	microbiota					77:86	gut microbiota	73:86	gut microbiota	73:86	Salecan confers anti-inflammatory effects in liver injury via regulating gut microbiota and its metabolites.					
36604080	5	53	theme	gut	841:843	arg1	microbiota					845:854	gut microbiota	841:854	gut microbiota	841:854	In addition, Salecan treatment modulated the composition of gut microbiota and antibiotic cocktail treatment indicated that the hepatoprotective effect of Salecan was dependent on the gut microbiota.					
36604080	3	54	theme	Salecan	564:570	arg1	supplementation					572:586	Salecan supplementation	564:586	Salecan supplementation in CCl4-induced liver injury	564:615	Here, the anti-inflammatory effects and the underlying mechanism of Salecan supplementation in CCl4-induced liver injury were investigated.					
36604080	1	55	from	approved	175:182	arg1	China					187:191	China	187:191	China	187:191	Salecan, a natural β-glucan and one of the novel food ingredients approved in China, has been shown a variety of positive health effects, yet the mechanism of liver injury remains poorly understood.					
36604080	6	56	theme	microbial	1078:1086	arg1	colonization					1088:1099	microbial colonization	1078:1099	microbial colonization	1078:1099	Fecal microbiota transplantation was used to further verify the mechanism, and we confirmed that microbial colonization partially alleviated liver injury.					
36604080	3	57	theme	supplementation	572:586	arg1	mechanism					551:559	the underlying mechanism	536:559	the underlying mechanism of Salecan supplementation in CCl4-induced liver injury	536:615	Here, the anti-inflammatory effects and the underlying mechanism of Salecan supplementation in CCl4-induced liver injury were investigated.					
36604080	3	57	theme	supplementation	572:586	arg1	effects					524:530	the anti-inflammatory effects	502:530	the anti-inflammatory effects	502:530	Here, the anti-inflammatory effects and the underlying mechanism of Salecan supplementation in CCl4-induced liver injury were investigated.					
36604080	4	58	theme	treatment	653:661	arg1	8 weeks					642:648	8 weeks	642:648	8 weeks of treatment	642:661	After 8 weeks of treatment, we observed that Salecan alleviated liver injury by regulating inflammatory response and M2 macrophage polarization.					
36604080	7	59	link	microbiota-derived	1145:1162	arg1	metabolites					1164:1174	microbiota-derived metabolites	1145:1174	microbiota-derived metabolites of Salecan	1145:1185	Besides, microbiota-derived metabolites of Salecan also contributed to the hepatoprotective and anti-inflammatory effect of Salecan against liver injury.					
36596372	7	0	theme	bacterial	1346:1354	arg1	Bifidobacterium					1363:1377	bacterial genera Bifidobacterium	1346:1377	bacterial genera Bifidobacterium	1346:1377	Notably, relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus displayed significantly positive correlations with the production of microbial fermentation-derived SCFAs.					
36596372	6	1	theme	mannose	1286:1292	arg1	pathway					1305:1311	mannose metabolism pathway	1286:1311	mannose metabolism pathway	1286:1311	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	3	2	theme	pre-	560:563	arg1	week					555:558	one week	551:558	one week pre- and post-weaning	551:580	Herein, fresh feces were collected at one week pre- and post-weaning as well as 3rd month post-weaning, respectively.					
36596372	2	3	with	association	418:428	arg1	microbiota					439:448	gut microbiota	435:448	gut microbiota	435:448	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	4	4	theme	increasing	698:707	arg1	concentrations					709:722	increasing concentrations	698:722	increasing concentrations of fecal short-chain fatty acids (SCFAs)	698:763	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	4	theme	increasing	698:707	arg1	utilizability					779:791	elevating utilizability	769:791	elevating utilizability of inulin	769:801	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	4	theme	increasing	698:707	arg1	substrate					821:829	a fermentation substrate	806:829	a fermentation substrate	806:829	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	2	5	theme	inulin	306:311	arg1	utilizability					313:325	inulin utilizability	306:325	inulin utilizability	306:325	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	1	6	theme	dietary	184:190	arg1	supplement					192:201	a promising dietary supplement	172:201	a promising dietary supplement due to its multiple health-promoting effects	172:246	Inulin is a typical kind of fermentable polysaccharide and has emerged as a promising dietary supplement due to its multiple health-promoting effects.					
36596372	6	7	theme	microbial	1043:1051	arg1	analyses					1064:1071	gut microbial functional analyses	1039:1071	gut microbial functional analyses	1039:1071	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	6	8	theme	phosphate	1184:1192	arg1	pathway					1194:1200	pentose phosphate pathway	1176:1200	pentose phosphate pathway	1176:1200	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	1	9	theme	due	203:205	arg1	supplement					192:201	a promising dietary supplement	172:201	a promising dietary supplement due to its multiple health-promoting effects	172:246	Inulin is a typical kind of fermentable polysaccharide and has emerged as a promising dietary supplement due to its multiple health-promoting effects.					
36596372	3	10	theme	post-weaning	603:614	arg1	month					597:601	3rd month post-weaning	593:614	3rd month post-weaning	593:614	Herein, fresh feces were collected at one week pre- and post-weaning as well as 3rd month post-weaning, respectively.					
36596372	5	11	theme	gut	909:911	arg1	composition					923:933	gut microbial composition	909:933	gut microbial composition	909:933	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	6	12	theme	galactose	1203:1211	arg1	pathway					1224:1230	galactose metabolism pathway	1203:1230	galactose metabolism pathway	1203:1230	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	4	13	theme	acids	751:755	arg1	concentrations					709:722	increasing concentrations	698:722	increasing concentrations of fecal short-chain fatty acids (SCFAs)	698:763	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	13	theme	acids	751:755	arg1	utilizability					779:791	elevating utilizability	769:791	elevating utilizability of inulin	769:801	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	13	theme	acids	751:755	arg1	substrate					821:829	a fermentation substrate	806:829	a fermentation substrate	806:829	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	7	14	theme	fermentation-derived	1504:1523	arg1	SCFAs					1525:1529	microbial fermentation-derived SCFAs	1494:1529	microbial fermentation-derived SCFAs	1494:1529	Notably, relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus displayed significantly positive correlations with the production of microbial fermentation-derived SCFAs.					
36596372	5	15	theme	gut	998:1000	arg1	development					1013:1023	gut microbiota development	998:1023	gut microbiota development	998:1023	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	8	16	theme	microbiota	1661:1670	arg1	development					1672:1682	gut microbiota development	1657:1682	gut microbiota development	1657:1682	Collectively, these findings offer novel insights into understanding inulin utilizability variations from the perspective of gut microbiota development.					
36596372	4	17	theme	short-chain	733:743	arg1	acids					751:755	fecal short-chain fatty acids	727:755	fecal short-chain fatty acids (SCFAs)	727:763	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	17	theme	short-chain	733:743	arg1	SCFAs					758:762	SCFAs	758:762	SCFAs	758:762	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	0	18	theme	gut	82:84	arg1	microbiota					86:95	gut microbiota	82:95	gut microbiota	82:95	Dynamic changes of inulin utilization associated with longitudinal development of gut microbiota.					
36596372	5	19	theme	microbial	961:969	arg1	diversity					981:989	microbial community diversity	961:989	microbial community diversity	961:989	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	4	20	theme	inulin	796:801	arg1	concentrations					709:722	increasing concentrations	698:722	increasing concentrations of fecal short-chain fatty acids (SCFAs)	698:763	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	20	theme	inulin	796:801	arg1	utilizability					779:791	elevating utilizability	769:791	elevating utilizability of inulin	769:801	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	20	theme	inulin	796:801	arg1	substrate					821:829	a fermentation substrate	806:829	a fermentation substrate	806:829	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	2	21	theme	dynamic	280:286	arg1	substrate					345:353	a fermentation substrate	330:353	a fermentation substrate	330:353	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	2	21	theme	dynamic	280:286	arg1	pattern					295:301	the dynamic change pattern	276:301	the dynamic change pattern of inulin utilizability	276:325	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	1	22	theme	fermentable	126:136	arg1	polysaccharide					138:151	fermentable polysaccharide	126:151	fermentable polysaccharide	126:151	Inulin is a typical kind of fermentable polysaccharide and has emerged as a promising dietary supplement due to its multiple health-promoting effects.					
36596372	6	23	theme	carbohydrate	1132:1143	arg1	pathway					1224:1230	galactose metabolism pathway	1203:1230	galactose metabolism pathway	1203:1230	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	6	23	theme	carbohydrate	1132:1143	arg1	pathway					1254:1260	butanoate metabolism pathway	1233:1260	butanoate metabolism pathway as well as fructose	1233:1280	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	6	23	theme	carbohydrate	1132:1143	arg1	pathway					1194:1200	pentose phosphate pathway	1176:1200	pentose phosphate pathway	1176:1200	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	6	23	theme	carbohydrate	1132:1143	arg1	pathways					1156:1163	carbohydrate metabolism pathways	1132:1163	carbohydrate metabolism pathways	1132:1163	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	6	23	theme	carbohydrate	1132:1143	arg1	pathway					1305:1311	mannose metabolism pathway	1286:1311	mannose metabolism pathway	1286:1311	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	2	24	theme	potential	408:416	arg1	association					418:428	its potential association	404:428	its potential association with gut microbiota	404:448	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	2	25	theme	native	468:473	arg1	models					479:484	Chinese Jinhua native pig models	453:484	Chinese Jinhua native pig models	453:484	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	0	26	theme	Dynamic	0:6	arg1	changes					8:14	Dynamic changes	0:14	Dynamic changes of inulin utilization	0:36	Dynamic changes of inulin utilization associated with longitudinal development of gut microbiota.					
36596372	6	27	theme	pathways	1156:1163	arg1	abundances					1118:1127	the relative abundances	1105:1127	the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway	1105:1311	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	2	28	theme	Chinese	453:459	arg1	models					479:484	Chinese Jinhua native pig models	453:484	Chinese Jinhua native pig models	453:484	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	5	29	theme	conspicuous	870:880	arg1	alteration					895:904	the conspicuous longitudinal alteration	866:904	the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity	866:989	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	7	30	theme	positive	1449:1456	arg1	correlations					1458:1469	positive correlations	1449:1469	positive correlations with the production of microbial fermentation-derived SCFAs	1449:1529	Notably, relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus displayed significantly positive correlations with the production of microbial fermentation-derived SCFAs.					
36596372	2	31	theme	longitudinal	490:501	arg1	analyses					503:510	longitudinal analyses	490:510	longitudinal analyses	490:510	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	0	32	theme	utilization	26:36	arg1	changes					8:14	Dynamic changes	0:14	Dynamic changes of inulin utilization	0:36	Dynamic changes of inulin utilization associated with longitudinal development of gut microbiota.					
36596372	4	33	dep	in	657:658	arg1	vitro					660:664	vitro	660:664	vitro	660:664	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	5	34	theme	Microbiomic	832:842	arg1	analyses					844:851	Microbiomic analyses	832:851	Microbiomic analyses	832:851	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	1	35	theme	typical	110:116	arg1	Inulin					98:103	Inulin	98:103	Inulin	98:103	Inulin is a typical kind of fermentable polysaccharide and has emerged as a promising dietary supplement due to its multiple health-promoting effects.					
36596372	1	35	theme	typical	110:116	arg1	kind					118:121	a typical kind	108:121	a typical kind of fermentable polysaccharide	108:151	Inulin is a typical kind of fermentable polysaccharide and has emerged as a promising dietary supplement due to its multiple health-promoting effects.					
36596372	6	36	theme	metabolism	1294:1303	arg1	pathway					1305:1311	mannose metabolism pathway	1286:1311	mannose metabolism pathway	1286:1311	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	1	37	theme	multiple	214:221	arg1	effects					240:246	its multiple health-promoting effects	210:246	its multiple health-promoting effects	210:246	Inulin is a typical kind of fermentable polysaccharide and has emerged as a promising dietary supplement due to its multiple health-promoting effects.					
36596372	6	38	theme	metabolism	1213:1222	arg1	pathway					1224:1230	galactose metabolism pathway	1203:1230	galactose metabolism pathway	1203:1230	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	8	39	theme	utilizability	1608:1620	arg1	variations					1622:1631	inulin utilizability variations	1601:1631	inulin utilizability variations from the perspective of gut microbiota development	1601:1682	Collectively, these findings offer novel insights into understanding inulin utilizability variations from the perspective of gut microbiota development.					
36596372	2	40	theme	gut	362:364	arg1	development					377:387	gut microbiota development	362:387	gut microbiota development	362:387	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	7	41	theme	genera	1356:1361	arg1	Bifidobacterium					1363:1377	bacterial genera Bifidobacterium	1346:1377	bacterial genera Bifidobacterium	1346:1377	Notably, relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus displayed significantly positive correlations with the production of microbial fermentation-derived SCFAs.					
36596372	2	42	theme	change	288:293	arg1	substrate					345:353	a fermentation substrate	330:353	a fermentation substrate	330:353	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	2	42	theme	change	288:293	arg1	pattern					295:301	the dynamic change pattern	276:301	the dynamic change pattern of inulin utilizability	276:325	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	7	43	link	fermentation-derived	1504:1523	arg1	SCFAs					1525:1529	microbial fermentation-derived SCFAs	1494:1529	microbial fermentation-derived SCFAs	1494:1529	Notably, relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus displayed significantly positive correlations with the production of microbial fermentation-derived SCFAs.					
36596372	6	44	theme	butanoate	1233:1241	arg1	pathway					1254:1260	butanoate metabolism pathway	1233:1260	butanoate metabolism pathway as well as fructose	1233:1280	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	5	45	theme	microbial	913:921	arg1	composition					923:933	gut microbial composition	909:933	gut microbial composition	909:933	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	4	46	theme	simulated	666:674	arg1	fermentation					676:687	in vitro simulated fermentation	657:687	in vitro simulated fermentation	657:687	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	7	47	theme	relative	1323:1330	arg1	abundances					1332:1341	relative abundances	1323:1341	relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus	1323:1423	Notably, relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus displayed significantly positive correlations with the production of microbial fermentation-derived SCFAs.					
36596372	2	48	theme	utilizability	313:325	arg1	substrate					345:353	a fermentation substrate	330:353	a fermentation substrate	330:353	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	2	48	theme	utilizability	313:325	arg1	pattern					295:301	the dynamic change pattern	276:301	the dynamic change pattern of inulin utilizability	276:325	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	1	49	theme	promising	174:182	arg1	supplement					192:201	a promising dietary supplement	172:201	a promising dietary supplement due to its multiple health-promoting effects	172:246	Inulin is a typical kind of fermentable polysaccharide and has emerged as a promising dietary supplement due to its multiple health-promoting effects.					
36596372	6	50	theme	functional	1053:1062	arg1	analyses					1064:1071	gut microbial functional analyses	1039:1071	gut microbial functional analyses	1039:1071	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	5	51	theme	longitudinal	882:893	arg1	alteration					895:904	the conspicuous longitudinal alteration	866:904	the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity	866:989	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	6	52	theme	gut	1039:1041	arg1	analyses					1064:1071	gut microbial functional analyses	1039:1071	gut microbial functional analyses	1039:1071	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	6	53	theme	metabolism	1243:1252	arg1	pathway					1254:1260	butanoate metabolism pathway	1233:1260	butanoate metabolism pathway as well as fructose	1233:1280	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	1	54	theme	polysaccharide	138:151	arg1	Inulin					98:103	Inulin	98:103	Inulin	98:103	Inulin is a typical kind of fermentable polysaccharide and has emerged as a promising dietary supplement due to its multiple health-promoting effects.					
36596372	1	54	theme	polysaccharide	138:151	arg1	kind					118:121	a typical kind	108:121	a typical kind of fermentable polysaccharide	108:151	Inulin is a typical kind of fermentable polysaccharide and has emerged as a promising dietary supplement due to its multiple health-promoting effects.					
36596372	3	55	theme	fresh	521:525	arg1	feces					527:531	fresh feces	521:531	fresh feces	521:531	Herein, fresh feces were collected at one week pre- and post-weaning as well as 3rd month post-weaning, respectively.					
36596372	4	56	theme	in	657:658	arg1	fermentation					676:687	in vitro simulated fermentation	657:687	in vitro simulated fermentation	657:687	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	7	57	with	correlations	1458:1469	arg1	production					1480:1489	the production	1476:1489	the production of microbial fermentation-derived SCFAs	1476:1529	Notably, relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus displayed significantly positive correlations with the production of microbial fermentation-derived SCFAs.					
36596372	5	58	theme	community	971:979	arg1	diversity					981:989	microbial community diversity	961:989	microbial community diversity	961:989	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	8	59	theme	gut	1657:1659	arg1	development					1672:1682	gut microbiota development	1657:1682	gut microbiota development	1657:1682	Collectively, these findings offer novel insights into understanding inulin utilizability variations from the perspective of gut microbiota development.					
36596372	4	60	theme	fatty	745:749	arg1	acids					751:755	fecal short-chain fatty acids	727:755	fecal short-chain fatty acids (SCFAs)	727:763	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	60	theme	fatty	745:749	arg1	SCFAs					758:762	SCFAs	758:762	SCFAs	758:762	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	5	61	from	alteration	895:904	arg1	composition					923:933	gut microbial composition	909:933	gut microbial composition	909:933	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	5	61	from	alteration	895:904	arg1	rise					953:956	a significant rise	939:956	a significant rise in microbial community diversity	939:989	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	6	62	theme	relative	1109:1116	arg1	abundances					1118:1127	the relative abundances	1105:1127	the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway	1105:1311	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	8	63	theme	development	1672:1682	arg1	perspective					1642:1652	the perspective	1638:1652	the perspective of gut microbiota development	1638:1682	Collectively, these findings offer novel insights into understanding inulin utilizability variations from the perspective of gut microbiota development.					
36596372	7	64	theme	SCFAs	1525:1529	arg1	production					1480:1489	the production	1476:1489	the production of microbial fermentation-derived SCFAs	1476:1529	Notably, relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus displayed significantly positive correlations with the production of microbial fermentation-derived SCFAs.					
36596372	8	65	from	perspective	1642:1652	arg1	variations					1622:1631	inulin utilizability variations	1601:1631	inulin utilizability variations from the perspective of gut microbiota development	1601:1682	Collectively, these findings offer novel insights into understanding inulin utilizability variations from the perspective of gut microbiota development.					
36596372	5	66	theme	significant	941:951	arg1	rise					953:956	a significant rise	939:956	a significant rise in microbial community diversity	939:989	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	7	67	theme	microbial	1494:1502	arg1	SCFAs					1525:1529	microbial fermentation-derived SCFAs	1494:1529	microbial fermentation-derived SCFAs	1494:1529	Notably, relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus displayed significantly positive correlations with the production of microbial fermentation-derived SCFAs.					
36596372	0	68	theme	microbiota	86:95	arg1	development					67:77	longitudinal development	54:77	longitudinal development of gut microbiota	54:95	Dynamic changes of inulin utilization associated with longitudinal development of gut microbiota.					
36596372	5	69	from	composition	923:933	arg1	diversity					981:989	microbial community diversity	961:989	microbial community diversity	961:989	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	6	70	theme	remarkable	1082:1091	arg1	increase					1093:1100	a remarkable increase	1080:1100	a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway	1080:1311	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	6	71	from	increase	1093:1100	arg1	abundances					1118:1127	the relative abundances	1105:1127	the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway	1105:1311	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	4	72	theme	fecal	727:731	arg1	acids					751:755	fecal short-chain fatty acids	727:755	fecal short-chain fatty acids (SCFAs)	727:763	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	72	theme	fecal	727:731	arg1	SCFAs					758:762	SCFAs	758:762	SCFAs	758:762	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	3	73	theme	post-weaning	569:580	arg1	week					555:558	one week	551:558	one week pre- and post-weaning	551:580	Herein, fresh feces were collected at one week pre- and post-weaning as well as 3rd month post-weaning, respectively.					
36596372	5	74	from	rise	953:956	arg1	diversity					981:989	microbial community diversity	961:989	microbial community diversity	961:989	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	6	75	theme	pentose	1176:1182	arg1	pathway					1194:1200	pentose phosphate pathway	1176:1200	pentose phosphate pathway	1176:1200	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	5	76	theme	microbiota	1002:1011	arg1	development					1013:1023	gut microbiota development	998:1023	gut microbiota development	998:1023	Microbiomic analyses demonstrated the conspicuous longitudinal alteration in gut microbial composition and a significant rise in microbial community diversity during gut microbiota development.					
36596372	2	77	theme	Jinhua	461:466	arg1	models					479:484	Chinese Jinhua native pig models	453:484	Chinese Jinhua native pig models	453:484	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	0	78	theme	inulin	19:24	arg1	utilization					26:36	inulin utilization	19:36	inulin utilization	19:36	Dynamic changes of inulin utilization associated with longitudinal development of gut microbiota.					
36596372	6	79	theme	metabolism	1145:1154	arg1	pathway					1224:1230	galactose metabolism pathway	1203:1230	galactose metabolism pathway	1203:1230	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	6	79	theme	metabolism	1145:1154	arg1	pathway					1254:1260	butanoate metabolism pathway	1233:1260	butanoate metabolism pathway as well as fructose	1233:1280	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	6	79	theme	metabolism	1145:1154	arg1	pathway					1194:1200	pentose phosphate pathway	1176:1200	pentose phosphate pathway	1176:1200	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	6	79	theme	metabolism	1145:1154	arg1	pathways					1156:1163	carbohydrate metabolism pathways	1132:1163	carbohydrate metabolism pathways	1132:1163	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	6	79	theme	metabolism	1145:1154	arg1	pathway					1305:1311	mannose metabolism pathway	1286:1311	mannose metabolism pathway	1286:1311	Furthermore, gut microbial functional analyses showed a remarkable increase in the relative abundances of carbohydrate metabolism pathways, including pentose phosphate pathway, galactose metabolism pathway, butanoate metabolism pathway as well as fructose and mannose metabolism pathway.					
36596372	4	80	theme	elevating	769:777	arg1	concentrations					709:722	increasing concentrations	698:722	increasing concentrations of fecal short-chain fatty acids (SCFAs)	698:763	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	80	theme	elevating	769:777	arg1	utilizability					779:791	elevating utilizability	769:791	elevating utilizability of inulin	769:801	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	80	theme	elevating	769:777	arg1	substrate					821:829	a fermentation substrate	806:829	a fermentation substrate	806:829	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	2	81	theme	gut	435:437	arg1	microbiota					439:448	gut microbiota	435:448	gut microbiota	435:448	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	7	82	theme	Enterococcus	1412:1423	arg1	abundances					1332:1341	relative abundances	1323:1341	relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus	1323:1423	Notably, relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus displayed significantly positive correlations with the production of microbial fermentation-derived SCFAs.					
36596372	0	83	theme	longitudinal	54:65	arg1	development					67:77	longitudinal development	54:77	longitudinal development of gut microbiota	54:95	Dynamic changes of inulin utilization associated with longitudinal development of gut microbiota.					
36596372	7	84	theme	Faecalibacterium	1391:1406	arg1	abundances					1332:1341	relative abundances	1323:1341	relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus	1323:1423	Notably, relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus displayed significantly positive correlations with the production of microbial fermentation-derived SCFAs.					
36596372	2	85	theme	pig	475:477	arg1	models					479:484	Chinese Jinhua native pig models	453:484	Chinese Jinhua native pig models	453:484	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	8	86	theme	novel	1567:1571	arg1	insights					1573:1580	novel insights	1567:1580	novel insights into understanding inulin utilizability variations from the perspective of gut microbiota development	1567:1682	Collectively, these findings offer novel insights into understanding inulin utilizability variations from the perspective of gut microbiota development.					
36596372	2	87	theme	fermentation	332:343	arg1	substrate					345:353	a fermentation substrate	330:353	a fermentation substrate	330:353	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	2	87	theme	fermentation	332:343	arg1	pattern					295:301	the dynamic change pattern	276:301	the dynamic change pattern of inulin utilizability	276:325	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
36596372	4	88	theme	fermentation	808:819	arg1	concentrations					709:722	increasing concentrations	698:722	increasing concentrations of fecal short-chain fatty acids (SCFAs)	698:763	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	88	theme	fermentation	808:819	arg1	utilizability					779:791	elevating utilizability	769:791	elevating utilizability of inulin	769:801	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	4	88	theme	fermentation	808:819	arg1	substrate					821:829	a fermentation substrate	806:829	a fermentation substrate	806:829	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	1	89	theme	health-promoting	223:238	arg1	effects					240:246	its multiple health-promoting effects	210:246	its multiple health-promoting effects	210:246	Inulin is a typical kind of fermentable polysaccharide and has emerged as a promising dietary supplement due to its multiple health-promoting effects.					
36596372	7	90	theme	Bifidobacterium	1363:1377	arg1	abundances					1332:1341	relative abundances	1323:1341	relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus	1323:1423	Notably, relative abundances of bacterial genera Bifidobacterium, Roseburia, Faecalibacterium and Enterococcus displayed significantly positive correlations with the production of microbial fermentation-derived SCFAs.					
36596372	4	91	theme	Targeted	631:638	arg1	metabolomics					640:651	Targeted metabolomics	631:651	Targeted metabolomics	631:651	Targeted metabolomics and in vitro simulated fermentation revealed increasing concentrations of fecal short-chain fatty acids (SCFAs) and elevating utilizability of inulin as a fermentation substrate.					
36596372	8	92	theme	inulin	1601:1606	arg1	variations					1622:1631	inulin utilizability variations	1601:1631	inulin utilizability variations from the perspective of gut microbiota development	1601:1682	Collectively, these findings offer novel insights into understanding inulin utilizability variations from the perspective of gut microbiota development.					
36596372	2	93	theme	microbiota	366:375	arg1	development					377:387	gut microbiota development	362:387	gut microbiota development	362:387	This study aimed to unveil the dynamic change pattern of inulin utilizability as a fermentation substrate during gut microbiota development and illuminate its potential association with gut microbiota in Chinese Jinhua native pig models via longitudinal analyses.					
35296709	2	0	theme	urea	300:303	arg1	effectiveness					283:295	the effectiveness	279:295	the effectiveness of urea	279:303	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	2	1	dep	necessary	396:404	arg1	availability					365:376	the availability	361:376	the availability of carbohydrates	361:393	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	2	1	dep	necessary	396:404	arg1	lack					312:315	the lack	308:315	the lack of synchronization between the nitrogen	308:355	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	2	1	dep	necessary	396:404	arg1	factor					259:264	Another factor	251:264	Another factor that affects the effectiveness of urea	251:303	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	5	2	theme	carbohydrate	791:802	arg1	source					804:809	the carbohydrate source	787:809	the carbohydrate source	787:809	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	12	3	contain	had	1776:1778	arg1	formula					1721:1727	The formula	1717:1727	The formula with cassava starch associated with urea (F17)	1717:1774	The formula with cassava starch associated with urea (F17) had a better synchronization during the degradation of its ingredients.					
35296709	12	3	contain	had	1776:1778	arg2	synchronization					1789:1803	a better synchronization	1780:1803	a better synchronization	1780:1803	The formula with cassava starch associated with urea (F17) had a better synchronization during the degradation of its ingredients.					
35296709	3	4	theme	different	611:619	arg1	sources					634:640	different carbohydrate sources	611:640	different carbohydrate sources	611:640	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	6	5	theme	gas	1082:1084	arg1	production					1086:1095	cumulative gas production	1071:1095	cumulative gas production	1071:1095	The supplements developed and two other commercial products based on extruded urea (UE) and polymer-coated urea (UP) were tested for solubility and cumulative gas production.					
35296709	9	6	theme	gas	1407:1409	arg1	production					1411:1420	The cumulative gas production	1392:1420	The cumulative gas production	1392:1420	The cumulative gas production showed a higher production related to the product F17 (p < 0.05).					
35296709	5	7	theme	urea	856:859	arg1	carbonate					878:886	calcium carbonate	870:886	calcium carbonate	870:886	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	5	7	theme	urea	856:859	arg1	urea					856:859	urea	856:859	urea	856:859	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	5	7	theme	urea	856:859	arg1	amounts					845:851	the same amounts	836:851	the same amounts of urea, sulfur, calcium carbonate	836:886	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	5	7	theme	urea	856:859	arg1	sulfur					862:867	sulfur	862:867	sulfur	862:867	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	7	8	theme	coating	1118:1124	arg1	process					1126:1132	the coating process	1114:1132	the coating process of the developed products	1114:1158	The wax used in the coating process of the developed products (F16 and F17) proved to be efficient in reducing the solubility of the ingredients used.					
35296709	11	9	theme	carnauba	1681:1688	arg1	wax					1690:1692	carnauba wax	1681:1692	carnauba wax	1681:1692	Therefore, is possible to reduce the solubility of urea using carnauba wax as a coating material.					
35296709	11	9	theme	carnauba	1681:1688	arg1	material					1707:1714	a coating material	1697:1714	a coating material	1697:1714	Therefore, is possible to reduce the solubility of urea using carnauba wax as a coating material.					
35296709	6	10	theme	other	957:961	arg1	products					974:981	two other commercial products	953:981	two other commercial products	953:981	The supplements developed and two other commercial products based on extruded urea (UE) and polymer-coated urea (UP) were tested for solubility and cumulative gas production.					
35296709	0	11	theme	controlled	82:91	arg1	release					93:99	controlled release	82:99	controlled release in ruminal fluid	82:116	A new feed additive composed of urea and soluble carbohydrate coated with wax for controlled release in ruminal fluid.					
35296709	8	12	theme	crude	1377:1381	arg1	protein					1383:1389	crude protein	1377:1389	crude protein	1377:1389	During chemical composition analysis it was verified that both supplements developed contained protein equivalent above 150% of crude protein.					
35296709	4	13	theme	sugarcane	684:692	arg1	source					721:726	a carbohydrate source	706:726	a carbohydrate source	706:726	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	4	13	theme	sugarcane	684:692	arg1	molasses					694:701	sugarcane molasses	684:701	sugarcane molasses	684:701	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	2	14	theme	synchronization	320:334	arg1	lack					312:315	the lack	308:315	the lack of synchronization between the nitrogen	308:355	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	2	14	theme	synchronization	320:334	arg1	availability					365:376	the availability	361:376	the availability of carbohydrates	361:393	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	2	14	theme	synchronization	320:334	arg1	factor					259:264	Another factor	251:264	Another factor that affects the effectiveness of urea	251:303	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	12	15	theme	ingredients	1835:1845	arg1	degradation					1816:1826	the degradation	1812:1826	the degradation of its ingredients	1812:1845	The formula with cassava starch associated with urea (F17) had a better synchronization during the degradation of its ingredients.					
35296709	7	16	theme	ingredients	1231:1241	arg1	solubility					1213:1222	the solubility	1209:1222	the solubility of the ingredients used	1209:1246	The wax used in the coating process of the developed products (F16 and F17) proved to be efficient in reducing the solubility of the ingredients used.					
35296709	2	17	theme	carbohydrates	381:393	arg1	lack					312:315	the lack	308:315	the lack of synchronization between the nitrogen	308:355	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	2	17	theme	carbohydrates	381:393	arg1	availability					365:376	the availability	361:376	the availability of carbohydrates	361:393	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	2	17	theme	carbohydrates	381:393	arg1	factor					259:264	Another factor	251:264	Another factor that affects the effectiveness of urea	251:303	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	9	18	theme	product	1464:1470	arg1	p < 0.05					1477:1484	p < 0.05	1477:1484	p < 0.05	1477:1484	The cumulative gas production showed a higher production related to the product F17 (p < 0.05).					
35296709	9	18	theme	product	1464:1470	arg1	F17					1472:1474	the product F17	1460:1474	the product F17 (p < 0.05)	1460:1485	The cumulative gas production showed a higher production related to the product F17 (p < 0.05).					
35296709	4	19	theme	products	654:661	arg1	One					643:645	One	643:645	One	643:645	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	4	19	theme	products	654:661	arg1	products					654:661	the products	650:661	the products developed	650:671	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	4	19	theme	products	654:661	arg1	F16					674:676	F16	674:676	F16	674:676	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	4	20	theme	cassava	756:762	arg1	starch					764:769	cassava starch	756:769	cassava starch	756:769	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	12	21	theme	cassava	1734:1740	arg1	starch					1742:1747	cassava starch	1734:1747	cassava starch associated with urea (F17)	1734:1774	The formula with cassava starch associated with urea (F17) had a better synchronization during the degradation of its ingredients.					
35296709	8	22	theme	composition	1265:1275	arg1	analysis					1277:1284	chemical composition analysis	1256:1284	chemical composition analysis	1256:1284	During chemical composition analysis it was verified that both supplements developed contained protein equivalent above 150% of crude protein.					
35296709	0	23	theme	ruminal	104:110	arg1	fluid					112:116	ruminal fluid	104:116	ruminal fluid	104:116	A new feed additive composed of urea and soluble carbohydrate coated with wax for controlled release in ruminal fluid.					
35296709	6	24	theme	cumulative	1071:1080	arg1	production					1086:1095	cumulative gas production	1071:1095	cumulative gas production	1071:1095	The supplements developed and two other commercial products based on extruded urea (UE) and polymer-coated urea (UP) were tested for solubility and cumulative gas production.					
35296709	5	25	theme	same	840:843	arg1	carbonate					878:886	calcium carbonate	870:886	calcium carbonate	870:886	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	5	25	theme	same	840:843	arg1	urea					856:859	urea	856:859	urea	856:859	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	5	25	theme	same	840:843	arg1	amounts					845:851	the same amounts	836:851	the same amounts of urea, sulfur, calcium carbonate	836:886	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	5	25	theme	same	840:843	arg1	sulfur					862:867	sulfur	862:867	sulfur	862:867	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	7	26	theme	products	1151:1158	arg1	process					1126:1132	the coating process	1114:1132	the coating process of the developed products	1114:1158	The wax used in the coating process of the developed products (F16 and F17) proved to be efficient in reducing the solubility of the ingredients used.					
35296709	6	27	theme	polymer-coated	1015:1028	arg1	UP					1036:1037	UP	1036:1037	UP	1036:1037	The supplements developed and two other commercial products based on extruded urea (UE) and polymer-coated urea (UP) were tested for solubility and cumulative gas production.					
35296709	6	27	theme	polymer-coated	1015:1028	arg1	urea					1030:1033	polymer-coated urea	1015:1033	polymer-coated urea (UP)	1015:1038	The supplements developed and two other commercial products based on extruded urea (UE) and polymer-coated urea (UP) were tested for solubility and cumulative gas production.					
35296709	6	28	theme	commercial	963:972	arg1	products					974:981	two other commercial products	953:981	two other commercial products	953:981	The supplements developed and two other commercial products based on extruded urea (UE) and polymer-coated urea (UP) were tested for solubility and cumulative gas production.					
35296709	8	29	contain	contained	1334:1342	arg1	supplements					1312:1322	both supplements	1307:1322	both supplements developed	1307:1332	During chemical composition analysis it was verified that both supplements developed contained protein equivalent above 150% of crude protein.					
35296709	8	29	contain	contained	1334:1342	arg2	equivalent					1352:1361	protein equivalent	1344:1361	protein equivalent	1344:1361	During chemical composition analysis it was verified that both supplements developed contained protein equivalent above 150% of crude protein.					
35296709	11	30	theme	coating	1699:1705	arg1	wax					1690:1692	carnauba wax	1681:1692	carnauba wax	1681:1692	Therefore, is possible to reduce the solubility of urea using carnauba wax as a coating material.					
35296709	11	30	theme	coating	1699:1705	arg1	material					1707:1714	a coating material	1697:1714	a coating material	1697:1714	Therefore, is possible to reduce the solubility of urea using carnauba wax as a coating material.					
35296709	0	31	theme	soluble	41:47	arg1	carbohydrate					49:60	soluble carbohydrate	41:60	soluble carbohydrate	41:60	A new feed additive composed of urea and soluble carbohydrate coated with wax for controlled release in ruminal fluid.					
35296709	9	32	theme	higher	1431:1436	arg1	production					1438:1447	a higher production	1429:1447	a higher production related to the product F17 (p < 0.05)	1429:1485	The cumulative gas production showed a higher production related to the product F17 (p < 0.05).					
35296709	5	33	contain	contained	826:834	arg2	sulfur					862:867	sulfur	862:867	sulfur	862:867	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	5	33	contain	contained	826:834	arg2	urea					856:859	urea	856:859	urea	856:859	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	5	33	contain	contained	826:834	arg2	carbonate					878:886	calcium carbonate	870:886	calcium carbonate	870:886	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	5	33	contain	contained	826:834	arg2	amounts					845:851	the same amounts	836:851	the same amounts of urea, sulfur, calcium carbonate	836:886	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	5	33	contain	contained	826:834	arg1	products					817:824	both products	812:824	both products	812:824	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	2	34	theme	better	410:415	arg1	development					417:427	better development	410:427	better development of the ruminal microbiota	410:453	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	3	35	theme	new	566:568	arg1	additives					582:590	two new nutritional additives	562:590	two new nutritional additives (F16 and F17)	562:604	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	3	35	theme	new	566:568	arg1	F17					601:603	F17	601:603	F17	601:603	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	3	35	theme	new	566:568	arg1	F16					593:595	F16	593:595	F16	593:595	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	7	36	theme	developed	1141:1149	arg1	products					1151:1158	the developed products	1137:1158	the developed products	1137:1158	The wax used in the coating process of the developed products (F16 and F17) proved to be efficient in reducing the solubility of the ingredients used.					
35296709	4	37	used	used	751:754	arg2	other					739:743	other	739:743	other	739:743	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	4	37	used	used	751:754	arg2	F17					746:748	F17	746:748	F17	746:748	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	3	38	theme	nutritional	570:580	arg1	additives					582:590	two new nutritional additives	562:590	two new nutritional additives (F16 and F17)	562:604	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	3	38	theme	nutritional	570:580	arg1	F17					601:603	F17	601:603	F17	601:603	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	3	38	theme	nutritional	570:580	arg1	F16					593:595	F16	593:595	F16	593:595	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	3	39	theme	urea	524:527	arg1	use					529:531	urea use	524:531	urea use	524:531	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	10	40	theme	chemical	1541:1548	arg1	integrity					1550:1558	the chemical integrity	1537:1558	the chemical integrity of the ingredients that make up the supplements developed	1537:1616	Through thermogravimetric analysis, it was found the chemical integrity of the ingredients that make up the supplements developed.					
35296709	8	41	theme	protein	1344:1350	arg1	equivalent					1352:1361	protein equivalent	1344:1361	protein equivalent	1344:1361	During chemical composition analysis it was verified that both supplements developed contained protein equivalent above 150% of crude protein.					
35296709	0	42	from	release	93:99	arg1	fluid					112:116	ruminal fluid	104:116	ruminal fluid	104:116	A new feed additive composed of urea and soluble carbohydrate coated with wax for controlled release in ruminal fluid.					
35296709	5	43	theme	carnauba	909:916	arg1	wax					918:920	carnauba wax	909:920	carnauba wax	909:920	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	12	44	theme	better	1782:1787	arg1	synchronization					1789:1803	a better synchronization	1780:1803	a better synchronization	1780:1803	The formula with cassava starch associated with urea (F17) had a better synchronization during the degradation of its ingredients.					
35296709	8	45	theme	chemical	1256:1263	arg1	analysis					1277:1284	chemical composition analysis	1256:1284	chemical composition analysis	1256:1284	During chemical composition analysis it was verified that both supplements developed contained protein equivalent above 150% of crude protein.					
35296709	9	46	theme	related	1449:1455	arg1	production					1438:1447	a higher production	1429:1447	a higher production related to the product F17 (p < 0.05)	1429:1485	The cumulative gas production showed a higher production related to the product F17 (p < 0.05).					
35296709	11	47	theme	urea	1670:1673	arg1	solubility					1656:1665	the solubility	1652:1665	the solubility of urea using carnauba wax as a coating material	1652:1714	Therefore, is possible to reduce the solubility of urea using carnauba wax as a coating material.					
35296709	7	48	dep	used	1106:1109	arg1	F17					1169:1171	F17	1169:1171	F17	1169:1171	The wax used in the coating process of the developed products (F16 and F17) proved to be efficient in reducing the solubility of the ingredients used.					
35296709	7	48	dep	used	1106:1109	arg1	wax					1102:1104	The wax	1098:1104	The wax used in the coating process of the developed products (F16 and F17)	1098:1172	The wax used in the coating process of the developed products (F16 and F17) proved to be efficient in reducing the solubility of the ingredients used.					
35296709	7	48	dep	used	1106:1109	arg1	F16					1161:1163	F16	1161:1163	F16	1161:1163	The wax used in the coating process of the developed products (F16 and F17) proved to be efficient in reducing the solubility of the ingredients used.					
35296709	9	49	theme	cumulative	1396:1405	arg1	production					1411:1420	The cumulative gas production	1392:1420	The cumulative gas production	1392:1420	The cumulative gas production showed a higher production related to the product F17 (p < 0.05).					
35296709	5	50	theme	calcium	870:876	arg1	carbonate					878:886	calcium carbonate	870:886	calcium carbonate	870:886	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	5	50	theme	calcium	870:876	arg1	urea					856:859	urea	856:859	urea	856:859	In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax.					
35296709	6	51	theme	extruded	992:999	arg1	urea					1001:1004	extruded urea	992:1004	extruded urea (UE)	992:1009	The supplements developed and two other commercial products based on extruded urea (UE) and polymer-coated urea (UP) were tested for solubility and cumulative gas production.					
35296709	6	51	theme	extruded	992:999	arg1	UE					1007:1008	UE	1007:1008	UE	1007:1008	The supplements developed and two other commercial products based on extruded urea (UE) and polymer-coated urea (UP) were tested for solubility and cumulative gas production.					
35296709	3	52	from	efficiency	510:519	arg1	use					529:531	urea use	524:531	urea use	524:531	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	2	53	theme	microbiota	444:453	arg1	development					417:427	better development	410:427	better development of the ruminal microbiota	410:453	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	10	54	theme	thermogravimetric	1496:1512	arg1	analysis					1514:1521	thermogravimetric analysis	1496:1521	thermogravimetric analysis	1496:1521	Through thermogravimetric analysis, it was found the chemical integrity of the ingredients that make up the supplements developed.					
35296709	3	55	theme	present	538:544	arg1	study					546:550	the present study	534:550	the present study	534:550	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	8	56	theme	protein	1383:1389	arg1	%					1372:1372	150%	1369:1372	150% of crude protein	1369:1389	During chemical composition analysis it was verified that both supplements developed contained protein equivalent above 150% of crude protein.					
35296709	8	56	theme	protein	1383:1389	arg1	protein					1383:1389	crude protein	1377:1389	crude protein	1377:1389	During chemical composition analysis it was verified that both supplements developed contained protein equivalent above 150% of crude protein.					
35296709	10	57	theme	ingredients	1567:1577	arg1	integrity					1550:1558	the chemical integrity	1537:1558	the chemical integrity of the ingredients that make up the supplements developed	1537:1616	Through thermogravimetric analysis, it was found the chemical integrity of the ingredients that make up the supplements developed.					
35296709	4	58	used	used	679:682	arg2	products					654:661	the products	650:661	the products developed	650:671	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	4	58	used	used	679:682	arg2	One					643:645	One	643:645	One	643:645	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	4	58	used	used	679:682	arg2	F16					674:676	F16	674:676	F16	674:676	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	3	59	dep	additives	582:590	arg1	additives					582:590	two new nutritional additives	562:590	two new nutritional additives (F16 and F17)	562:604	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	3	59	dep	additives	582:590	arg1	F17					601:603	F17	601:603	F17	601:603	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	3	59	dep	additives	582:590	arg1	F16					593:595	F16	593:595	F16	593:595	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	2	60	theme	ruminal	436:442	arg1	microbiota					444:453	the ruminal microbiota	432:453	the ruminal microbiota	432:453	Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota.					
35296709	3	61	theme	carbohydrate	621:632	arg1	sources					634:640	different carbohydrate sources	611:640	different carbohydrate sources	611:640	In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources.					
35296709	4	62	theme	carbohydrate	708:719	arg1	source					721:726	a carbohydrate source	706:726	a carbohydrate source	706:726	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	4	62	theme	carbohydrate	708:719	arg1	molasses					694:701	sugarcane molasses	684:701	sugarcane molasses	684:701	One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch.					
35296709	12	63	with	formula	1721:1727	arg1	starch					1742:1747	cassava starch	1734:1747	cassava starch associated with urea (F17)	1734:1774	The formula with cassava starch associated with urea (F17) had a better synchronization during the degradation of its ingredients.					
36172867	0	0	theme	Atherosclerotic	71:85	arg1	Disease					87:93	Atherosclerotic Disease	71:93	Atherosclerotic Disease	71:93	Ex Vivo Study Using Diffusion Tensor Imaging to Identify Biomarkers of Atherosclerotic Disease in Human Cadaveric Carotid Arteries.					
36172867	7	1	theme	different	1308:1316	arg1	investigations					1318:1331	These different investigations	1302:1331	These different investigations	1302:1331	CONCLUSIONS These different investigations point to the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development.					
36172867	5	2	from	decrease	922:929	arg1	anisotropy					945:954	fractional anisotropy	934:954	fractional anisotropy	934:954	RESULTS All vessels displayed a dramatic decrease in fractional anisotropy compared with healthy animal arterial tissue, while the mean diffusivity was sensitive to regions of advanced disease.					
36172867	3	3	dep	metrics	690:696	arg1	diffusivity					730:740	mean diffusivity	725:740	mean diffusivity	725:740	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	3	dep	metrics	690:696	arg1	metrics					690:696	diffusion tensor imaging derived metrics	657:696	diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity)	657:741	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	3	dep	metrics	690:696	arg1	anisotropy					710:719	fractional anisotropy	699:719	fractional anisotropy	699:719	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	6	4	dep	anisotropy	1132:1141	arg1	r>0.7					1144:1148	r>0.7	1144:1148	r>0.7	1144:1148	Elastin content strongly correlated with both fractional anisotropy (r>0.7, P<0.001) and mean diffusivity (r>-0.79, P<0.0002), and the thickened intima was also distinguishable from arterial media by these metrics.					
36172867	6	4	dep	anisotropy	1132:1141	arg1	P<0.001					1151:1157	P<0.001	1151:1157	P<0.001	1151:1157	Elastin content strongly correlated with both fractional anisotropy (r>0.7, P<0.001) and mean diffusivity (r>-0.79, P<0.0002), and the thickened intima was also distinguishable from arterial media by these metrics.					
36172867	5	5	dep	RESULTS	881:887	arg1	displayed					901:909	displayed	901:909	displayed	901:909	RESULTS All vessels displayed a dramatic decrease in fractional anisotropy compared with healthy animal arterial tissue, while the mean diffusivity was sensitive to regions of advanced disease.					
36172867	6	6	from	media	1266:1270	arg1	intima					1220:1225	the thickened intima	1206:1225	the thickened intima	1206:1225	Elastin content strongly correlated with both fractional anisotropy (r>0.7, P<0.001) and mean diffusivity (r>-0.79, P<0.0002), and the thickened intima was also distinguishable from arterial media by these metrics.					
36172867	6	6	from	media	1266:1270	arg1	distinguishable					1236:1250	distinguishable	1236:1250	distinguishable	1236:1250	Elastin content strongly correlated with both fractional anisotropy (r>0.7, P<0.001) and mean diffusivity (r>-0.79, P<0.0002), and the thickened intima was also distinguishable from arterial media by these metrics.					
36172867	3	7	theme	unsupervised	531:542	arg1	segmentation					544:555	unsupervised segmentation	531:555	unsupervised segmentation	531:555	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	8	from	influence	576:584	arg1	diffusivity					730:740	mean diffusivity	725:740	mean diffusivity	725:740	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	8	from	influence	576:584	arg1	metrics					690:696	diffusion tensor imaging derived metrics	657:696	diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity)	657:741	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	8	from	influence	576:584	arg1	anisotropy					710:719	fractional anisotropy	699:719	fractional anisotropy	699:719	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	9	theme	mean	725:728	arg1	diffusivity					730:740	mean diffusivity	725:740	mean diffusivity	725:740	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	9	theme	mean	725:728	arg1	metrics					690:696	diffusion tensor imaging derived metrics	657:696	diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity)	657:741	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	10	theme	cell	608:611	arg1	density					613:619	cell density	608:619	cell density	608:619	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	10	theme	cell	608:611	arg1	elastin					589:595	elastin	589:595	elastin	589:595	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	7	11	theme	imaging	1376:1382	arg1	potential					1346:1354	the potential	1342:1354	the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development	1342:1486	CONCLUSIONS These different investigations point to the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development.					
36172867	3	12	link	derived	682:688	arg1	diffusivity					730:740	mean diffusivity	725:740	mean diffusivity	725:740	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	12	link	derived	682:688	arg1	metrics					690:696	diffusion tensor imaging derived metrics	657:696	diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity)	657:741	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	12	link	derived	682:688	arg1	anisotropy					710:719	fractional anisotropy	699:719	fractional anisotropy	699:719	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	1	13	theme	microstructural	248:262	arg1	composition					264:274	microstructural composition	248:274	microstructural composition	248:274	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	0	14	theme	Disease	87:93	arg1	Biomarkers					57:66	Biomarkers	57:66	Biomarkers of Atherosclerotic Disease in Human Cadaveric Carotid Arteries	57:129	Ex Vivo Study Using Diffusion Tensor Imaging to Identify Biomarkers of Atherosclerotic Disease in Human Cadaveric Carotid Arteries.					
36172867	6	15	theme	arterial	1257:1264	arg1	media					1266:1270	arterial media	1257:1270	arterial media	1257:1270	Elastin content strongly correlated with both fractional anisotropy (r>0.7, P<0.001) and mean diffusivity (r>-0.79, P<0.0002), and the thickened intima was also distinguishable from arterial media by these metrics.					
36172867	1	16	dep	BACKGROUND	132:141	arg1	aims					154:157	aims	154:157	aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries	154:351	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	6	17	theme	Elastin	1075:1081	arg1	content					1083:1089	Elastin content	1075:1089	Elastin content	1075:1089	Elastin content strongly correlated with both fractional anisotropy (r>0.7, P<0.001) and mean diffusivity (r>-0.79, P<0.0002), and the thickened intima was also distinguishable from arterial media by these metrics.					
36172867	0	18	theme	Cadaveric	104:112	arg1	Arteries					122:129	Human Cadaveric Carotid Arteries	98:129	Human Cadaveric Carotid Arteries	98:129	Ex Vivo Study Using Diffusion Tensor Imaging to Identify Biomarkers of Atherosclerotic Disease in Human Cadaveric Carotid Arteries.					
36172867	5	19	theme	animal	978:983	arg1	tissue					994:999	healthy animal arterial tissue	970:999	healthy animal arterial tissue	970:999	RESULTS All vessels displayed a dramatic decrease in fractional anisotropy compared with healthy animal arterial tissue, while the mean diffusivity was sensitive to regions of advanced disease.					
36172867	7	20	theme	late-stage	1458:1467	arg1	development					1476:1486	early and late-stage lesion development	1448:1486	early and late-stage lesion development	1448:1486	CONCLUSIONS These different investigations point to the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development.					
36172867	6	21	theme	fractional	1121:1130	arg1	anisotropy					1132:1141	fractional anisotropy	1121:1141	fractional anisotropy (r>0.7, P<0.001)	1121:1158	Elastin content strongly correlated with both fractional anisotropy (r>0.7, P<0.001) and mean diffusivity (r>-0.79, P<0.0002), and the thickened intima was also distinguishable from arterial media by these metrics.					
36172867	4	22	theme	microstructural	816:830	arg1	components					832:841	microstructural components	816:841	microstructural components	816:841	Early stage atherosclerotic features were also investigated in terms of microstructural components and diffusion tensor imaging metrics.					
36172867	7	23	theme	arterial	1415:1422	arg1	progression					1432:1442	arterial disease progression	1415:1442	arterial disease progression	1415:1442	CONCLUSIONS These different investigations point to the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development.					
36172867	5	24	theme	dramatic	913:920	arg1	decrease					922:929	a dramatic decrease	911:929	a dramatic decrease in fractional anisotropy	911:954	RESULTS All vessels displayed a dramatic decrease in fractional anisotropy compared with healthy animal arterial tissue, while the mean diffusivity was sensitive to regions of advanced disease.					
36172867	0	25	theme	Human	98:102	arg1	Arteries					122:129	Human Cadaveric Carotid Arteries	98:129	Human Cadaveric Carotid Arteries	98:129	Ex Vivo Study Using Diffusion Tensor Imaging to Identify Biomarkers of Atherosclerotic Disease in Human Cadaveric Carotid Arteries.					
36172867	4	26	theme	tensor	857:862	arg1	imaging					864:870	diffusion tensor imaging	847:870	diffusion tensor imaging metrics	847:878	Early stage atherosclerotic features were also investigated in terms of microstructural components and diffusion tensor imaging metrics.					
36172867	2	27	theme	healthy	459:465	arg1	regions					480:486	healthy and diseased regions	459:486	healthy and diseased regions	459:486	METHODS In this study, whole human carotid arteries were investigated both anatomically and by comparing healthy and diseased regions.					
36172867	5	28	theme	advanced	1057:1064	arg1	disease					1066:1072	advanced disease	1057:1072	advanced disease	1057:1072	RESULTS All vessels displayed a dramatic decrease in fractional anisotropy compared with healthy animal arterial tissue, while the mean diffusivity was sensitive to regions of advanced disease.					
36172867	5	29	theme	disease	1066:1072	arg1	regions					1046:1052	regions	1046:1052	regions of advanced disease	1046:1072	RESULTS All vessels displayed a dramatic decrease in fractional anisotropy compared with healthy animal arterial tissue, while the mean diffusivity was sensitive to regions of advanced disease.					
36172867	7	30	dep	CONCLUSIONS	1290:1300	arg1	point					1333:1337	point	1333:1337	point to the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development	1333:1486	CONCLUSIONS These different investigations point to the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development.					
36172867	2	31	theme	carotid	389:395	arg1	arteries					397:404	whole human carotid arteries	377:404	whole human carotid arteries	377:404	METHODS In this study, whole human carotid arteries were investigated both anatomically and by comparing healthy and diseased regions.					
36172867	0	32	theme	Ex	0:1	arg1	Study					8:12	Ex Vivo Study	0:12	Ex Vivo Study Using Diffusion Tensor Imaging	0:43	Ex Vivo Study Using Diffusion Tensor Imaging to Identify Biomarkers of Atherosclerotic Disease in Human Cadaveric Carotid Arteries.					
36172867	7	33	theme	progression	1432:1442	arg1	characteristics					1396:1410	characteristics	1396:1410	characteristics of arterial disease progression	1396:1442	CONCLUSIONS These different investigations point to the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development.					
36172867	2	34	theme	human	383:387	arg1	arteries					397:404	whole human carotid arteries	377:404	whole human carotid arteries	377:404	METHODS In this study, whole human carotid arteries were investigated both anatomically and by comparing healthy and diseased regions.					
36172867	1	35	theme	morphological	280:292	arg1	arrangement					294:304	morphological arrangement	280:304	morphological arrangement	280:304	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	7	36	theme	disease	1424:1430	arg1	progression					1432:1442	arterial disease progression	1415:1442	arterial disease progression	1415:1442	CONCLUSIONS These different investigations point to the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development.					
36172867	3	37	theme	elastin	589:595	arg1	influence					576:584	the influence	572:584	the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity)	572:741	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	4	38	theme	metrics	872:878	arg1	terms					807:811	terms	807:811	terms of microstructural components and diffusion tensor imaging metrics	807:878	Early stage atherosclerotic features were also investigated in terms of microstructural components and diffusion tensor imaging metrics.					
36172867	1	39	theme	ex	187:188	arg1	imaging					212:218	ex vivo diffusion tensor imaging	187:218	ex vivo diffusion tensor imaging	187:218	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	4	40	theme	diffusion	847:855	arg1	imaging					864:870	diffusion tensor imaging	847:870	diffusion tensor imaging metrics	847:878	Early stage atherosclerotic features were also investigated in terms of microstructural components and diffusion tensor imaging metrics.					
36172867	0	41	theme	Carotid	114:120	arg1	Arteries					122:129	Human Cadaveric Carotid Arteries	98:129	Human Cadaveric Carotid Arteries	98:129	Ex Vivo Study Using Diffusion Tensor Imaging to Identify Biomarkers of Atherosclerotic Disease in Human Cadaveric Carotid Arteries.					
36172867	5	42	theme	healthy	970:976	arg1	tissue					994:999	healthy animal arterial tissue	970:999	healthy animal arterial tissue	970:999	RESULTS All vessels displayed a dramatic decrease in fractional anisotropy compared with healthy animal arterial tissue, while the mean diffusivity was sensitive to regions of advanced disease.					
36172867	3	43	theme	Nonrigid	489:496	arg1	registration					504:515	Nonrigid image registration	489:515	Nonrigid image registration	489:515	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	0	44	theme	Tensor	30:35	arg1	Imaging					37:43	Diffusion Tensor Imaging	20:43	Diffusion Tensor Imaging	20:43	Ex Vivo Study Using Diffusion Tensor Imaging to Identify Biomarkers of Atherosclerotic Disease in Human Cadaveric Carotid Arteries.					
36172867	5	45	theme	arterial	985:992	arg1	tissue					994:999	healthy animal arterial tissue	970:999	healthy animal arterial tissue	970:999	RESULTS All vessels displayed a dramatic decrease in fractional anisotropy compared with healthy animal arterial tissue, while the mean diffusivity was sensitive to regions of advanced disease.					
36172867	3	46	theme	derived	682:688	arg1	diffusivity					730:740	mean diffusivity	725:740	mean diffusivity	725:740	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	46	theme	derived	682:688	arg1	metrics					690:696	diffusion tensor imaging derived metrics	657:696	diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity)	657:741	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	46	theme	derived	682:688	arg1	anisotropy					710:719	fractional anisotropy	699:719	fractional anisotropy	699:719	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	2	47	dep	both	424:427	arg1	anatomically					429:440	anatomically	429:440	anatomically	429:440	METHODS In this study, whole human carotid arteries were investigated both anatomically and by comparing healthy and diseased regions.					
36172867	5	48	theme	fractional	934:943	arg1	anisotropy					945:954	fractional anisotropy	934:954	fractional anisotropy	934:954	RESULTS All vessels displayed a dramatic decrease in fractional anisotropy compared with healthy animal arterial tissue, while the mean diffusivity was sensitive to regions of advanced disease.					
36172867	1	49	theme	diffusion	195:203	arg1	imaging					212:218	ex vivo diffusion tensor imaging	187:218	ex vivo diffusion tensor imaging	187:218	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	0	50	theme	Diffusion	20:28	arg1	Imaging					37:43	Diffusion Tensor Imaging	20:43	Diffusion Tensor Imaging	20:43	Ex Vivo Study Using Diffusion Tensor Imaging to Identify Biomarkers of Atherosclerotic Disease in Human Cadaveric Carotid Arteries.					
36172867	1	51	theme	aged	309:312	arg1	arteries					344:351	aged human atherosclerotic carotid arteries	309:351	aged human atherosclerotic carotid arteries	309:351	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	7	52	theme	lesion	1469:1474	arg1	development					1476:1486	early and late-stage lesion development	1448:1486	early and late-stage lesion development	1448:1486	CONCLUSIONS These different investigations point to the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development.					
36172867	0	53	from	Biomarkers	57:66	arg1	Arteries					122:129	Human Cadaveric Carotid Arteries	98:129	Human Cadaveric Carotid Arteries	98:129	Ex Vivo Study Using Diffusion Tensor Imaging to Identify Biomarkers of Atherosclerotic Disease in Human Cadaveric Carotid Arteries.					
36172867	2	54	dep	investigated	411:422	arg1	both					424:427	both	424:427	both	424:427	METHODS In this study, whole human carotid arteries were investigated both anatomically and by comparing healthy and diseased regions.					
36172867	2	54	dep	investigated	411:422	arg1	comparing					449:457	comparing	449:457	comparing healthy and diseased regions	449:486	METHODS In this study, whole human carotid arteries were investigated both anatomically and by comparing healthy and diseased regions.					
36172867	1	55	theme	tensor	205:210	arg1	imaging					212:218	ex vivo diffusion tensor imaging	187:218	ex vivo diffusion tensor imaging	187:218	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	1	56	theme	human	314:318	arg1	arteries					344:351	aged human atherosclerotic carotid arteries	309:351	aged human atherosclerotic carotid arteries	309:351	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	3	57	theme	tensor	667:672	arg1	imaging					674:680	diffusion tensor imaging	657:680	diffusion tensor imaging	657:680	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	58	used	used	521:524	arg2	registration					504:515	Nonrigid image registration	489:515	Nonrigid image registration	489:515	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	4	59	theme	Early	744:748	arg1	features					772:779	Early stage atherosclerotic features	744:779	Early stage atherosclerotic features	744:779	Early stage atherosclerotic features were also investigated in terms of microstructural components and diffusion tensor imaging metrics.					
36172867	2	60	theme	diseased	471:478	arg1	regions					480:486	healthy and diseased regions	459:486	healthy and diseased regions	459:486	METHODS In this study, whole human carotid arteries were investigated both anatomically and by comparing healthy and diseased regions.					
36172867	1	61	theme	atherosclerotic	320:334	arg1	arteries					344:351	aged human atherosclerotic carotid arteries	309:351	aged human atherosclerotic carotid arteries	309:351	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	3	62	theme	fractional	699:708	arg1	metrics					690:696	diffusion tensor imaging derived metrics	657:696	diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity)	657:741	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	3	62	theme	fractional	699:708	arg1	anisotropy					710:719	fractional anisotropy	699:719	fractional anisotropy	699:719	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	7	63	theme	early	1448:1452	arg1	development					1476:1486	early and late-stage lesion development	1448:1486	early and late-stage lesion development	1448:1486	CONCLUSIONS These different investigations point to the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development.					
36172867	3	64	theme	diffusion	657:665	arg1	imaging					674:680	diffusion tensor imaging	657:680	diffusion tensor imaging	657:680	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	1	65	theme	carotid	336:342	arg1	arteries					344:351	aged human atherosclerotic carotid arteries	309:351	aged human atherosclerotic carotid arteries	309:351	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	1	66	dep	composition	264:274	arg1	the					244:246	the	244:246	the	244:246	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	6	67	dep	r>-0.79	1182:1188	arg1	P<0.0002					1191:1198	P<0.0002	1191:1198	P<0.0002	1191:1198	Elastin content strongly correlated with both fractional anisotropy (r>0.7, P<0.001) and mean diffusivity (r>-0.79, P<0.0002), and the thickened intima was also distinguishable from arterial media by these metrics.					
36172867	4	68	theme	imaging	864:870	arg1	metrics					872:878	diffusion tensor imaging metrics	847:878	diffusion tensor imaging metrics	847:878	Early stage atherosclerotic features were also investigated in terms of microstructural components and diffusion tensor imaging metrics.					
36172867	3	69	theme	image	498:502	arg1	registration					504:515	Nonrigid image registration	489:515	Nonrigid image registration	489:515	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
36172867	5	70	theme	mean	1012:1015	arg1	sensitive					1033:1041	sensitive	1033:1041	sensitive	1033:1041	RESULTS All vessels displayed a dramatic decrease in fractional anisotropy compared with healthy animal arterial tissue, while the mean diffusivity was sensitive to regions of advanced disease.					
36172867	5	70	theme	mean	1012:1015	arg1	diffusivity					1017:1027	the mean diffusivity	1008:1027	the mean diffusivity	1008:1027	RESULTS All vessels displayed a dramatic decrease in fractional anisotropy compared with healthy animal arterial tissue, while the mean diffusivity was sensitive to regions of advanced disease.					
36172867	1	71	theme	imaging	212:218	arg1	potential					174:182	the potential	170:182	the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries	170:351	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	6	72	theme	thickened	1210:1218	arg1	intima					1220:1225	the thickened intima	1206:1225	the thickened intima	1206:1225	Elastin content strongly correlated with both fractional anisotropy (r>0.7, P<0.001) and mean diffusivity (r>-0.79, P<0.0002), and the thickened intima was also distinguishable from arterial media by these metrics.					
36172867	6	72	theme	thickened	1210:1218	arg1	distinguishable					1236:1250	distinguishable	1236:1250	distinguishable	1236:1250	Elastin content strongly correlated with both fractional anisotropy (r>0.7, P<0.001) and mean diffusivity (r>-0.79, P<0.0002), and the thickened intima was also distinguishable from arterial media by these metrics.					
36172867	4	73	theme	components	832:841	arg1	terms					807:811	terms	807:811	terms of microstructural components and diffusion tensor imaging metrics	807:878	Early stage atherosclerotic features were also investigated in terms of microstructural components and diffusion tensor imaging metrics.					
36172867	4	74	theme	stage	750:754	arg1	features					772:779	Early stage atherosclerotic features	744:779	Early stage atherosclerotic features	744:779	Early stage atherosclerotic features were also investigated in terms of microstructural components and diffusion tensor imaging metrics.					
36172867	4	75	theme	atherosclerotic	756:770	arg1	features					772:779	Early stage atherosclerotic features	744:779	Early stage atherosclerotic features	744:779	Early stage atherosclerotic features were also investigated in terms of microstructural components and diffusion tensor imaging metrics.					
36172867	7	76	theme	diffusion	1359:1367	arg1	imaging					1376:1382	diffusion tensor imaging	1359:1382	diffusion tensor imaging	1359:1382	CONCLUSIONS These different investigations point to the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development.					
36172867	1	77	dep	ex	187:188	arg1	vivo					190:193	vivo	190:193	vivo	190:193	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	7	78	theme	tensor	1369:1374	arg1	imaging					1376:1382	diffusion tensor imaging	1359:1382	diffusion tensor imaging	1359:1382	CONCLUSIONS These different investigations point to the potential of diffusion tensor imaging to identify characteristics of arterial disease progression, at early and late-stage lesion development.					
36172867	2	79	theme	whole	377:381	arg1	arteries					397:404	whole human carotid arteries	377:404	whole human carotid arteries	377:404	METHODS In this study, whole human carotid arteries were investigated both anatomically and by comparing healthy and diseased regions.					
36172867	1	80	theme	arteries	344:351	arg1	arrangement					294:304	morphological arrangement	280:304	morphological arrangement	280:304	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	1	80	theme	arteries	344:351	arg1	composition					264:274	microstructural composition	248:274	microstructural composition	248:274	BACKGROUND This study aims to address the potential of ex vivo diffusion tensor imaging to provide insight into the microstructural composition and morphological arrangement of aged human atherosclerotic carotid arteries.					
36172867	0	81	dep	Ex	0:1	arg1	Vivo					3:6	Vivo	3:6	Vivo	3:6	Ex Vivo Study Using Diffusion Tensor Imaging to Identify Biomarkers of Atherosclerotic Disease in Human Cadaveric Carotid Arteries.					
36172867	6	82	theme	mean	1164:1167	arg1	r>-0.79					1182:1188	r>-0.79	1182:1188	r>-0.79	1182:1188	Elastin content strongly correlated with both fractional anisotropy (r>0.7, P<0.001) and mean diffusivity (r>-0.79, P<0.0002), and the thickened intima was also distinguishable from arterial media by these metrics.					
36172867	6	82	theme	mean	1164:1167	arg1	diffusivity					1169:1179	mean diffusivity	1164:1179	mean diffusivity (r>-0.79, P<0.0002)	1164:1199	Elastin content strongly correlated with both fractional anisotropy (r>0.7, P<0.001) and mean diffusivity (r>-0.79, P<0.0002), and the thickened intima was also distinguishable from arterial media by these metrics.					
36172867	3	83	dep	derived	682:688	arg1	imaging					674:680	diffusion tensor imaging	657:680	diffusion tensor imaging	657:680	Nonrigid image registration was used with unsupervised segmentation to investigate the influence of elastin, collagen, cell density, glycosaminoglycans, and calcium on diffusion tensor imaging derived metrics (fractional anisotropy and mean diffusivity).					
35217084	2	0	theme	nanofiber	423:431	arg1	film					433:436	chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film	376:436	chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations	376:506	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	4	1	theme	DPPH	757:760	arg1	radical					767:773	DPPH free radical	757:773	the DPPH free radical scavenging rate	753:789	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	1	2	theme	treatment	149:157	arg1	cost					127:130	The high cost	118:130	The high cost of wound healing treatment	118:157	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	1	2	theme	treatment	149:157	arg1	recovery					169:176	the slow recovery	160:176	the slow recovery of wounds	160:186	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	1	2	theme	treatment	149:157	arg1	uncertainty					197:207	the uncertainty	193:207	the uncertainty of being affected by the body's physiological activities	193:264	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	2	3	dep	in	487:488	arg1	vitro					490:494	vitro	490:494	vitro	490:494	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	2	4	dep	in	475:476	arg1	vivo					478:481	vivo	478:481	vivo	478:481	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	6	5	theme	film	993:996	arg1	heal					998:1001	film heal	993:1001	film heal	993:1001	Animal experiments have proved that wounds treated with CS-PVP-DHQ nanofiber film heal faster.					
35217084	4	6	theme	free	762:765	arg1	radical					767:773	DPPH free radical	757:773	the DPPH free radical scavenging rate	753:789	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	8	7	from	potential	1265:1273	arg1	applications					1292:1303	wound healing applications	1278:1303	wound healing applications	1278:1303	Therefore, the nanofiber film herein show great potential in wound healing applications.					
35217084	0	8	theme	wound	103:107	arg1	healing					109:115	wound healing	103:115	wound healing	103:115	Fabrication of chitosan/PVP/dihydroquercetin nanocomposite film for in vitro and in vivo evaluation of wound healing.					
35217084	3	9	theme	prepared	584:591	arg1	film					593:596	the prepared film	580:596	the prepared film	580:596	The results show that the prepared film has good morphology, thermal stability and hydrophilicity.					
35217084	4	10	theme	radical	767:773	arg1	rate					786:789	the DPPH free radical scavenging rate	753:789	the DPPH free radical scavenging rate	753:789	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	5	11	theme	cytotoxicity	848:859	arg1	test					861:864	MTT cytotoxicity test	844:864	MTT cytotoxicity test	844:864	MTT cytotoxicity test proved that the film is non-toxic to Hacat cells.					
35217084	5	12	theme	MTT	844:846	arg1	test					861:864	MTT cytotoxicity test	844:864	MTT cytotoxicity test	844:864	MTT cytotoxicity test proved that the film is non-toxic to Hacat cells.					
35217084	4	13	theme	antioxidant	822:832	arg1	activity					834:841	antioxidant activity	822:841	antioxidant activity	822:841	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	1	14	from	burden	287:292	arg1	health					304:309	public health	297:309	public health	297:309	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	7	15	theme	vascular	1142:1149	arg1	factor					1170:1175	vascular endothelial growth factor VEGF and CD31	1142:1189	vascular endothelial growth factor VEGF and CD31 to promote wound healing	1142:1214	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	7	15	theme	vascular	1142:1149	arg1	pan-keratin					1129:1139	pan-keratin	1129:1139	pan-keratin	1129:1139	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	3	16	contain	has	598:600	arg1	film					593:596	the prepared film	580:596	the prepared film	580:596	The results show that the prepared film has good morphology, thermal stability and hydrophilicity.					
35217084	3	16	contain	has	598:600	arg2	stability					627:635	thermal stability	619:635	thermal stability	619:635	The results show that the prepared film has good morphology, thermal stability and hydrophilicity.					
35217084	3	16	contain	has	598:600	arg2	morphology					607:616	good morphology	602:616	good morphology	602:616	The results show that the prepared film has good morphology, thermal stability and hydrophilicity.					
35217084	3	16	contain	has	598:600	arg2	hydrophilicity					641:654	hydrophilicity	641:654	hydrophilicity	641:654	The results show that the prepared film has good morphology, thermal stability and hydrophilicity.					
35217084	2	17	theme	wound	446:450	arg1	excipients					452:461	wound excipients	446:461	wound excipients	446:461	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	3	18	theme	thermal	619:625	arg1	stability					627:635	thermal stability	619:635	thermal stability	619:635	The results show that the prepared film has good morphology, thermal stability and hydrophilicity.					
35217084	0	19	theme	healing	109:115	arg1	evaluation					89:98	in vitro and in vivo evaluation	68:98	in vitro and in vivo evaluation of wound healing	68:115	Fabrication of chitosan/PVP/dihydroquercetin nanocomposite film for in vitro and in vivo evaluation of wound healing.					
35217084	3	20	theme	good	602:605	arg1	morphology					607:616	good morphology	602:616	good morphology	602:616	The results show that the prepared film has good morphology, thermal stability and hydrophilicity.					
35217084	1	21	theme	slow	164:167	arg1	recovery					169:176	the slow recovery	160:176	the slow recovery of wounds	160:186	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	5	22	theme	Hacat	903:907	arg1	cells					909:913	Hacat cells	903:913	Hacat cells	903:913	MTT cytotoxicity test proved that the film is non-toxic to Hacat cells.					
35217084	7	23	theme	growth	1163:1168	arg1	factor					1170:1175	vascular endothelial growth factor VEGF and CD31	1142:1189	vascular endothelial growth factor VEGF and CD31 to promote wound healing	1142:1214	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	7	23	theme	growth	1163:1168	arg1	pan-keratin					1129:1139	pan-keratin	1129:1139	pan-keratin	1129:1139	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	4	24	theme	scavenging	775:784	arg1	rate					786:789	the DPPH free radical scavenging rate	753:789	the DPPH free radical scavenging rate	753:789	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	2	25	theme	PVP	391:393	arg1	film					433:436	chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film	376:436	chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations	376:506	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	1	26	theme	physiological	241:253	arg1	activities					255:264	the body's physiological activities	230:264	the body's physiological activities	230:264	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	8	27	theme	great	1259:1263	arg1	potential					1265:1273	great potential	1259:1273	great potential in wound healing applications	1259:1303	Therefore, the nanofiber film herein show great potential in wound healing applications.					
35217084	7	28	theme	nanofiber	1051:1059	arg1	film					1061:1064	the composite nanofiber film	1037:1064	the composite nanofiber film	1037:1064	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	2	29	theme	in	475:476	arg1	evaluations					496:506	in vivo and in vitro evaluations	475:506	in vivo and in vitro evaluations	475:506	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	0	30	dep	in	81:82	arg1	vivo					84:87	vivo	84:87	vivo	84:87	Fabrication of chitosan/PVP/dihydroquercetin nanocomposite film for in vitro and in vivo evaluation of wound healing.					
35217084	4	31	contain	has	818:820	arg1	film					813:816	the fiber film	803:816	the fiber film	803:816	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	4	31	contain	has	818:820	arg2	activity					834:841	antioxidant activity	822:841	antioxidant activity	822:841	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	1	32	theme	wounds	181:186	arg1	cost					127:130	The high cost	118:130	The high cost of wound healing treatment	118:157	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	1	32	theme	wounds	181:186	arg1	recovery					169:176	the slow recovery	160:176	the slow recovery of wounds	160:186	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	1	32	theme	wounds	181:186	arg1	uncertainty					197:207	the uncertainty	193:207	the uncertainty of being affected by the body's physiological activities	193:264	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	0	33	theme	chitosan/PVP/dihydroquercetin	15:43	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of chitosan/PVP/dihydroquercetin	0:43	Fabrication of chitosan/PVP/dihydroquercetin nanocomposite film for in vitro and in vivo evaluation of wound healing.					
35217084	8	34	theme	healing	1284:1290	arg1	applications					1292:1303	wound healing applications	1278:1303	wound healing applications	1278:1303	Therefore, the nanofiber film herein show great potential in wound healing applications.					
35217084	4	35	dep	In	657:658	arg1	vitro					660:664	vitro	660:664	vitro	660:664	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	7	36	theme	endothelial	1151:1161	arg1	factor					1170:1175	vascular endothelial growth factor VEGF and CD31	1142:1189	vascular endothelial growth factor VEGF and CD31 to promote wound healing	1142:1214	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	7	36	theme	endothelial	1151:1161	arg1	pan-keratin					1129:1139	pan-keratin	1129:1139	pan-keratin	1129:1139	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	2	37	from	effective	537:545	arg1	wounds					550:555	wounds	550:555	wounds	550:555	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	2	38	theme	chitosan	376:383	arg1	film					433:436	chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film	376:436	chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations	376:506	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	7	39	theme	composite	1041:1049	arg1	film					1061:1064	the composite nanofiber film	1037:1064	the composite nanofiber film	1037:1064	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	1	40	theme	serious	279:285	arg1	burden					287:292	a serious burden	277:292	a serious burden on public health	277:309	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	4	41	theme	In	657:658	arg1	studies					666:672	In vitro studies	657:672	In vitro studies	657:672	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	0	42	dep	in	68:69	arg1	vitro					71:75	vitro	71:75	vitro	71:75	Fabrication of chitosan/PVP/dihydroquercetin nanocomposite film for in vitro and in vivo evaluation of wound healing.					
35217084	6	43	theme	Animal	916:921	arg1	experiments					923:933	Animal experiments	916:933	Animal experiments	916:933	Animal experiments have proved that wounds treated with CS-PVP-DHQ nanofiber film heal faster.					
35217084	2	44	theme	dihydroquercetin	400:415	arg1	film					433:436	chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film	376:436	chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations	376:506	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	7	45	theme	pan-keratin	1129:1139	arg1	expression					1115:1124	the expression	1111:1124	the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing	1111:1214	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	0	46	theme	in	68:69	arg1	evaluation					89:98	in vitro and in vivo evaluation	68:98	in vitro and in vivo evaluation of wound healing	68:115	Fabrication of chitosan/PVP/dihydroquercetin nanocomposite film for in vitro and in vivo evaluation of wound healing.					
35217084	2	47	theme	in	487:488	arg1	evaluations					496:506	in vivo and in vitro evaluations	475:506	in vivo and in vitro evaluations	475:506	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	1	48	theme	high	122:125	arg1	cost					127:130	The high cost	118:130	The high cost of wound healing treatment	118:157	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	1	49	theme	public	297:302	arg1	health					304:309	public health	297:309	public health	297:309	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	4	50	theme	antibacterial	697:709	arg1	activity					711:718	antibacterial activity	697:718	antibacterial activity against S.aureus and E.coli	697:746	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	8	51	theme	wound	1278:1282	arg1	healing					1284:1290	wound healing	1278:1290	wound healing applications	1278:1303	Therefore, the nanofiber film herein show great potential in wound healing applications.					
35217084	7	52	dep	factor	1170:1175	arg1	VEGF					1177:1180	VEGF	1177:1180	VEGF	1177:1180	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	7	52	dep	factor	1170:1175	arg1	promote					1194:1200	promote	1194:1200	to promote wound healing	1191:1214	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	7	52	dep	factor	1170:1175	arg1	CD31					1186:1189	CD31	1186:1189	CD31	1186:1189	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	2	53	from	wounds	550:555	arg1	effective					537:545	effective	537:545	effective	537:545	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	2	53	from	wounds	550:555	arg1	film					529:532	the film	525:532	the film	525:532	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	8	54	theme	nanofiber	1232:1240	arg1	film					1242:1245	the nanofiber film	1228:1245	the nanofiber film	1228:1245	Therefore, the nanofiber film herein show great potential in wound healing applications.					
35217084	4	55	contain	has	693:695	arg1	it					690:691	it	690:691	it	690:691	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	4	55	contain	has	693:695	arg2	activity					711:718	antibacterial activity	697:718	antibacterial activity against S.aureus and E.coli	697:746	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	0	56	theme	in	81:82	arg1	evaluation					89:98	in vitro and in vivo evaluation	68:98	in vitro and in vivo evaluation of wound healing	68:115	Fabrication of chitosan/PVP/dihydroquercetin nanocomposite film for in vitro and in vivo evaluation of wound healing.					
35217084	4	57	theme	fiber	807:811	arg1	film					813:816	the fiber film	803:816	the fiber film	803:816	In vitro studies have shown that it has antibacterial activity against S.aureus and E.coli, and the DPPH free radical scavenging rate proves that the fiber film has antioxidant activity.					
35217084	7	58	theme	wound	1202:1206	arg1	healing					1208:1214	wound healing	1202:1214	wound healing	1202:1214	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	1	59	theme	wound	135:139	arg1	healing					141:147	wound healing	135:147	wound healing treatment	135:157	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
35217084	2	60	theme	film	433:436	arg1	characterization					356:371	characterization	356:371	characterization	356:371	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	2	60	theme	film	433:436	arg1	preparation					340:350	the preparation	336:350	the preparation	336:350	In this work, we report the preparation and characterization of chitosan (CS), PVP, and dihydroquercetin (DHQ) nanofiber film used as wound excipients, as well as in vivo and in vitro evaluations, and verify that the film is effective in wounds.					
35217084	7	61	theme	autophagy	1078:1086	arg1	pathway					1088:1094	autophagy pathway	1078:1094	autophagy pathway	1078:1094	This article also studied the composite nanofiber film by inducing autophagy pathway and increasing the expression of pan-keratin, vascular endothelial growth factor VEGF and CD31 to promote wound healing.					
35217084	1	62	theme	healing	141:147	arg1	treatment					149:157	wound healing treatment	135:157	wound healing treatment	135:157	The high cost of wound healing treatment, the slow recovery of wounds, and the uncertainty of being affected by the body's physiological activities constitute a serious burden on public health.					
37169054	8	0	theme	pore	1152:1155	arg1	volume					1157:1162	pore volume	1152:1162	pore volume	1152:1162	Cs-g-PAN/ZnO possesses a specific surface area of 20.23 m2/g with a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1.					
37169054	5	1	theme	ZnO	698:700	arg1	nanoparticles					702:714	ZnO nanoparticles	698:714	ZnO nanoparticles into the Cs-g-PAN matrix	698:739	The simple ultrasonic technique was used for doping ZnO nanoparticles into the Cs-g-PAN matrix to prepare chitosan-grafted polyacrylonitrile/ZnO (Cs-g-PAN/ZnO).					
37169054	10	2	theme	dose	1352:1355	arg1	effect					1297:1302	The effect	1293:1302	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II)	1293:1463	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	11	3	theme	removal	1489:1495	arg1	efficiency					1497:1506	The removal efficiency	1485:1506	The removal efficiency	1485:1506	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	10	4	theme	iron	1455:1458	arg1	removal					1444:1450	the removal	1440:1450	the removal of iron (II)	1440:1463	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	1	5	theme	serious	194:200	arg1	concern					202:208	serious concern	194:208	serious concern in wastewater environmental sustainability and human health	194:268	The highly poisonous, non-biodegradable heavy metals present serious concern in wastewater environmental sustainability and human health.					
37169054	2	6	theme	kind	341:344	arg1	treatment					323:331	the treatment	319:331	the treatment of this kind of water	319:353	Using adsorption is an effective technology for the treatment of this kind of water.					
37169054	14	7	theme	Fe	2028:2029	arg1	monolayer					2035:2043	Fe(II) monolayer	2028:2043	Fe(II) monolayer	2028:2043	Suggesting the formation of Fe(II) monolayer over the adsorbent surface and the rate-limiting step is probably controlled by chemisorption through sharing the electrons between Fe+2 and the prepared catalyst.					
37169054	11	8	theme	room	1656:1659	arg1	temperature					1661:1671	room temperature	1656:1671	room temperature	1656:1671	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	5	9	theme	chitosan-grafted	752:767	arg1	Cs-g-PAN/ZnO					792:803	Cs-g-PAN/ZnO	792:803	Cs-g-PAN/ZnO	792:803	The simple ultrasonic technique was used for doping ZnO nanoparticles into the Cs-g-PAN matrix to prepare chitosan-grafted polyacrylonitrile/ZnO (Cs-g-PAN/ZnO).					
37169054	5	9	theme	chitosan-grafted	752:767	arg1	polyacrylonitrile/ZnO					769:789	chitosan-grafted polyacrylonitrile/ZnO	752:789	chitosan-grafted polyacrylonitrile/ZnO (Cs-g-PAN/ZnO)	752:804	The simple ultrasonic technique was used for doping ZnO nanoparticles into the Cs-g-PAN matrix to prepare chitosan-grafted polyacrylonitrile/ZnO (Cs-g-PAN/ZnO).					
37169054	1	10	theme	environmental	224:236	arg1	sustainability					238:251	wastewater environmental sustainability	213:251	wastewater environmental sustainability	213:251	The highly poisonous, non-biodegradable heavy metals present serious concern in wastewater environmental sustainability and human health.					
37169054	12	11	theme	synthesized	1810:1820	arg1	nanomaterials					1822:1834	the synthesized nanomaterials	1806:1834	the synthesized nanomaterials	1806:1834	Langmuir and Freundlich's isothermal and kinetic studies have been analyzed to determine the adsorption mechanism of Fe(II) ions on the synthesized nanomaterials.					
37169054	5	12	theme	Cs-g-PAN	725:732	arg1	matrix					734:739	the Cs-g-PAN matrix	721:739	the Cs-g-PAN matrix	721:739	The simple ultrasonic technique was used for doping ZnO nanoparticles into the Cs-g-PAN matrix to prepare chitosan-grafted polyacrylonitrile/ZnO (Cs-g-PAN/ZnO).					
37169054	10	13	from	effect	1297:1302	arg1	removal					1444:1450	the removal	1440:1450	the removal of iron (II)	1440:1463	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	10	14	theme	adsorbent	1342:1350	arg1	0.01-0.3 g					1358:1367	0.01-0.3 g	1358:1367	0.01-0.3 g	1358:1367	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	10	14	theme	adsorbent	1342:1350	arg1	dose					1352:1355	adsorbent dose	1342:1355	adsorbent dose (0.01-0.3 g)	1342:1368	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	14	15	theme	prepared	2190:2197	arg1	catalyst					2199:2206	the prepared catalyst	2186:2206	the prepared catalyst	2186:2206	Suggesting the formation of Fe(II) monolayer over the adsorbent surface and the rate-limiting step is probably controlled by chemisorption through sharing the electrons between Fe+2 and the prepared catalyst.					
37169054	7	16	theme	prepared	1038:1045	arg1	materials					1047:1055	the prepared materials	1034:1055	the prepared materials	1034:1055	XRD, FTIR, SEM, TEM, BET, EDS, and TGA measurements were conducted to confirm the morphological and structural properties of the prepared materials.					
37169054	13	17	theme	pseudo-second-order	1946:1964	arg1	kinetics					1975:1982	the Langmuir model and pseudo-second-order reaction kinetics	1923:1982	kinetics	1975:1982	The adsorption process of Fe(II) over the surface of prepared catalysts proceeded via the Langmuir model and pseudo-second-order reaction kinetics with R2 > 0.99.					
37169054	1	18	from	concern	202:208	arg1	sustainability					238:251	wastewater environmental sustainability	213:251	wastewater environmental sustainability	213:251	The highly poisonous, non-biodegradable heavy metals present serious concern in wastewater environmental sustainability and human health.					
37169054	1	18	from	concern	202:208	arg1	health					263:268	human health	257:268	human health	257:268	The highly poisonous, non-biodegradable heavy metals present serious concern in wastewater environmental sustainability and human health.					
37169054	1	19	theme	poisonous	144:152	arg1	metals					179:184	The highly poisonous, non-biodegradable heavy metals	133:184	The highly poisonous, non-biodegradable heavy metals	133:184	The highly poisonous, non-biodegradable heavy metals present serious concern in wastewater environmental sustainability and human health.					
37169054	11	20	theme	concentration	1598:1610	arg1	0.93 mg/L					1612:1620	adsorbate concentration 0.93 mg/L	1588:1620	adsorbate concentration 0.93 mg/L	1588:1620	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	10	21	theme	degrees	1397:1403	arg1	effect					1297:1302	The effect	1293:1302	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II)	1293:1463	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	12	22	theme	Fe	1791:1792	arg1	ions					1798:1801	Fe(II) ions	1791:1801	Fe(II) ions	1791:1801	Langmuir and Freundlich's isothermal and kinetic studies have been analyzed to determine the adsorption mechanism of Fe(II) ions on the synthesized nanomaterials.					
37169054	10	23	theme	different	1375:1383	arg1	degrees					1397:1403	different temperature degrees	1375:1403	different temperature degrees (278, 288, 298, 308, and 318 K)	1375:1435	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	11	24	theme	adsorbent	1627:1635	arg1	0.05 g/L					1644:1651	adsorbent dosage 0.05 g/L	1627:1651	adsorbent dosage 0.05 g/L	1627:1651	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	7	25	theme	TGA	944:946	arg1	measurements					948:959	TGA measurements	944:959	TGA measurements	944:959	XRD, FTIR, SEM, TEM, BET, EDS, and TGA measurements were conducted to confirm the morphological and structural properties of the prepared materials.					
37169054	3	26	theme	developing	367:376	arg1	adsorbents					407:416	developing efficient and cost-effective adsorbents	367:416	developing efficient and cost-effective adsorbents	367:416	Therefore, developing efficient and cost-effective adsorbents considers a significant and an emerging topic in the field the water purification.					
37169054	4	27	theme	grafted	510:516	arg1	Cs-g-PAN					537:544	Cs-g-PAN	537:544	Cs-g-PAN	537:544	Chitosan grafted polyacrylonitrile (Cs-g-PAN) was facially fabricated via graft polymerization using ammonium persulfate (APS) as the initiator.					
37169054	4	27	theme	grafted	510:516	arg1	polyacrylonitrile					518:534	Chitosan grafted polyacrylonitrile	501:534	Chitosan grafted polyacrylonitrile (Cs-g-PAN)	501:545	Chitosan grafted polyacrylonitrile (Cs-g-PAN) was facially fabricated via graft polymerization using ammonium persulfate (APS) as the initiator.					
37169054	13	28	theme	prepared	1890:1897	arg1	catalysts					1899:1907	prepared catalysts	1890:1907	prepared catalysts	1890:1907	The adsorption process of Fe(II) over the surface of prepared catalysts proceeded via the Langmuir model and pseudo-second-order reaction kinetics with R2 > 0.99.					
37169054	0	29	theme	Chitosan-g-polyacrylonitrile	0:27	arg1	nano-composite					33:46	Chitosan-g-polyacrylonitrile ZnO nano-composite	0:46	Chitosan-g-polyacrylonitrile ZnO nano-composite	0:46	Chitosan-g-polyacrylonitrile ZnO nano-composite, synthesis and characterization as new and good adsorbent for Iron from groundwater.					
37169054	4	30	theme	ammonium	602:609	arg1	persulfate					611:620	ammonium persulfate	602:620	ammonium persulfate (APS)	602:626	Chitosan grafted polyacrylonitrile (Cs-g-PAN) was facially fabricated via graft polymerization using ammonium persulfate (APS) as the initiator.					
37169054	4	30	theme	ammonium	602:609	arg1	initiator					635:643	the initiator	631:643	the initiator	631:643	Chitosan grafted polyacrylonitrile (Cs-g-PAN) was facially fabricated via graft polymerization using ammonium persulfate (APS) as the initiator.					
37169054	4	30	theme	ammonium	602:609	arg1	APS					623:625	APS	623:625	APS	623:625	Chitosan grafted polyacrylonitrile (Cs-g-PAN) was facially fabricated via graft polymerization using ammonium persulfate (APS) as the initiator.					
37169054	6	31	theme	PAN	860:862	arg1	nanocomposite					843:855	nanocomposite	843:855	nanocomposite	843:855	For comparative study, pure ZnO and nanocomposite of PAN doped with ZnO (PAN/ZnO) were also prepared.					
37169054	6	31	theme	PAN	860:862	arg1	ZnO					835:837	pure ZnO	830:837	pure ZnO	830:837	For comparative study, pure ZnO and nanocomposite of PAN doped with ZnO (PAN/ZnO) were also prepared.					
37169054	6	31	theme	PAN	860:862	arg1	PAN					860:862	PAN	860:862	PAN doped with ZnO (PAN/ZnO)	860:887	For comparative study, pure ZnO and nanocomposite of PAN doped with ZnO (PAN/ZnO) were also prepared.					
37169054	8	32	theme	surface	1092:1098	arg1	area					1100:1103	a specific surface area	1081:1103	a specific surface area of 20.23 m2/g	1081:1117	Cs-g-PAN/ZnO possesses a specific surface area of 20.23 m2/g with a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1.					
37169054	0	33	dep	new	83:85	arg1	adsorbent					96:104	adsorbent	96:104	adsorbent	96:104	Chitosan-g-polyacrylonitrile ZnO nano-composite, synthesis and characterization as new and good adsorbent for Iron from groundwater.					
37169054	3	34	theme	emerging	449:456	arg1	topic					458:462	an emerging topic	446:462	an emerging topic in the field	446:475	Therefore, developing efficient and cost-effective adsorbents considers a significant and an emerging topic in the field the water purification.					
37169054	11	35	theme	contact	1567:1573	arg1	30 min					1580:1585	contact time 30 min	1567:1585	contact time 30 min	1567:1585	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	3	36	theme	water	481:485	arg1	purification					487:498	the water purification	477:498	the water purification	477:498	Therefore, developing efficient and cost-effective adsorbents considers a significant and an emerging topic in the field the water purification.					
37169054	14	37	theme	monolayer	2035:2043	arg1	formation					2015:2023	the formation	2011:2023	the formation of Fe(II) monolayer over the adsorbent surface	2011:2070	Suggesting the formation of Fe(II) monolayer over the adsorbent surface and the rate-limiting step is probably controlled by chemisorption through sharing the electrons between Fe+2 and the prepared catalyst.					
37169054	12	38	theme	isothermal	1700:1709	arg1	studies					1723:1729	Langmuir and Freundlich's isothermal and kinetic studies	1674:1729	Langmuir and Freundlich's isothermal and kinetic studies	1674:1729	Langmuir and Freundlich's isothermal and kinetic studies have been analyzed to determine the adsorption mechanism of Fe(II) ions on the synthesized nanomaterials.					
37169054	11	39	theme	adsorbate	1588:1596	arg1	0.93 mg/L					1612:1620	adsorbate concentration 0.93 mg/L	1588:1620	adsorbate concentration 0.93 mg/L	1588:1620	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	6	40	theme	comparative	811:821	arg1	study					823:827	comparative study	811:827	comparative study	811:827	For comparative study, pure ZnO and nanocomposite of PAN doped with ZnO (PAN/ZnO) were also prepared.					
37169054	1	41	theme	human	257:261	arg1	health					263:268	human health	257:268	human health	257:268	The highly poisonous, non-biodegradable heavy metals present serious concern in wastewater environmental sustainability and human health.					
37169054	8	42	theme	pore	1126:1129	arg1	size					1131:1134	a pore size	1124:1134	a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1	1124:1178	Cs-g-PAN/ZnO possesses a specific surface area of 20.23 m2/g with a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1.					
37169054	14	43	theme	rate-limiting	2080:2092	arg1	step					2094:2097	the rate-limiting step	2076:2097	the rate-limiting step	2076:2097	Suggesting the formation of Fe(II) monolayer over the adsorbent surface and the rate-limiting step is probably controlled by chemisorption through sharing the electrons between Fe+2 and the prepared catalyst.					
37169054	5	44	used	used	682:685	arg2	technique					668:676	The simple ultrasonic technique	646:676	The simple ultrasonic technique	646:676	The simple ultrasonic technique was used for doping ZnO nanoparticles into the Cs-g-PAN matrix to prepare chitosan-grafted polyacrylonitrile/ZnO (Cs-g-PAN/ZnO).					
37169054	10	45	theme	contact	1317:1323	arg1	time					1325:1328	contact time	1317:1328	contact time (5-60 min)	1317:1339	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	10	45	theme	contact	1317:1323	arg1	5-60 min					1331:1338	5-60 min	1331:1338	5-60 min	1331:1338	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	14	46	theme	adsorbent	2054:2062	arg1	surface					2064:2070	the adsorbent surface	2050:2070	the adsorbent surface	2050:2070	Suggesting the formation of Fe(II) monolayer over the adsorbent surface and the rate-limiting step is probably controlled by chemisorption through sharing the electrons between Fe+2 and the prepared catalyst.					
37169054	9	47	theme	adsorption	1185:1194	arg1	behavior					1196:1203	The adsorption behavior	1181:1203	The adsorption behavior toward Fe(II) as a pollutant for groundwater	1181:1248	The adsorption behavior toward Fe(II) as a pollutant for groundwater was studied for the synthesized materials.					
37169054	8	48	theme	volume	1157:1162	arg1	size					1131:1134	a pore size	1124:1134	a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1	1124:1178	Cs-g-PAN/ZnO possesses a specific surface area of 20.23 m2/g with a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1.					
37169054	13	49	theme	adsorption	1841:1850	arg1	process					1852:1858	The adsorption process	1837:1858	The adsorption process of Fe(II) over the surface of prepared catalysts	1837:1907	The adsorption process of Fe(II) over the surface of prepared catalysts proceeded via the Langmuir model and pseudo-second-order reaction kinetics with R2 > 0.99.					
37169054	8	50	theme	0.16 cm3 g-1	1167:1178	arg1	31.58 nm					1139:1146	31.58 nm	1139:1146	31.58 nm	1139:1146	Cs-g-PAN/ZnO possesses a specific surface area of 20.23 m2/g with a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1.					
37169054	8	50	theme	0.16 cm3 g-1	1167:1178	arg1	volume					1157:1162	pore volume	1152:1162	pore volume	1152:1162	Cs-g-PAN/ZnO possesses a specific surface area of 20.23 m2/g with a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1.					
37169054	11	51	from	temperature	1661:1671	arg1	30 min					1580:1585	contact time 30 min	1567:1585	contact time 30 min	1567:1585	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	11	51	from	temperature	1661:1671	arg1	0.05 g/L					1644:1651	adsorbent dosage 0.05 g/L	1627:1651	adsorbent dosage 0.05 g/L	1627:1651	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	11	51	from	temperature	1661:1671	arg1	pH = 7					1559:1564	pH = 7	1559:1564	pH = 7	1559:1564	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	11	51	from	temperature	1661:1671	arg1	0.93 mg/L					1612:1620	adsorbate concentration 0.93 mg/L	1588:1620	adsorbate concentration 0.93 mg/L	1588:1620	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	11	52	theme	optimum	1537:1543	arg1	condition					1545:1553	the optimum condition	1533:1553	the optimum condition	1533:1553	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	1	53	theme	wastewater	213:222	arg1	sustainability					238:251	wastewater environmental sustainability	213:251	wastewater environmental sustainability	213:251	The highly poisonous, non-biodegradable heavy metals present serious concern in wastewater environmental sustainability and human health.					
37169054	12	54	theme	ions	1798:1801	arg1	mechanism					1778:1786	the adsorption mechanism	1763:1786	the adsorption mechanism of Fe(II) ions	1763:1801	Langmuir and Freundlich's isothermal and kinetic studies have been analyzed to determine the adsorption mechanism of Fe(II) ions on the synthesized nanomaterials.					
37169054	4	55	theme	Chitosan	501:508	arg1	Cs-g-PAN					537:544	Cs-g-PAN	537:544	Cs-g-PAN	537:544	Chitosan grafted polyacrylonitrile (Cs-g-PAN) was facially fabricated via graft polymerization using ammonium persulfate (APS) as the initiator.					
37169054	4	55	theme	Chitosan	501:508	arg1	polyacrylonitrile					518:534	Chitosan grafted polyacrylonitrile	501:534	Chitosan grafted polyacrylonitrile (Cs-g-PAN)	501:545	Chitosan grafted polyacrylonitrile (Cs-g-PAN) was facially fabricated via graft polymerization using ammonium persulfate (APS) as the initiator.					
37169054	7	56	theme	materials	1047:1055	arg1	properties					1020:1029	the morphological and structural properties	987:1029	the morphological and structural properties of the prepared materials	987:1055	XRD, FTIR, SEM, TEM, BET, EDS, and TGA measurements were conducted to confirm the morphological and structural properties of the prepared materials.					
37169054	13	57	theme	Langmuir	1927:1934	arg1	model					1936:1940	the Langmuir model and pseudo-second-order reaction kinetics	1923:1982	model	1936:1940	The adsorption process of Fe(II) over the surface of prepared catalysts proceeded via the Langmuir model and pseudo-second-order reaction kinetics with R2 > 0.99.					
37169054	0	58	from	groundwater	120:130	arg1	Iron					110:113	Iron	110:113	Iron from groundwater	110:130	Chitosan-g-polyacrylonitrile ZnO nano-composite, synthesis and characterization as new and good adsorbent for Iron from groundwater.					
37169054	2	59	theme	effective	294:302	arg1	technology					304:313	an effective technology	291:313	an effective technology for the treatment of this kind of water	291:353	Using adsorption is an effective technology for the treatment of this kind of water.					
37169054	7	60	theme	structural	1009:1018	arg1	properties					1020:1029	the morphological and structural properties	987:1029	the morphological and structural properties of the prepared materials	987:1055	XRD, FTIR, SEM, TEM, BET, EDS, and TGA measurements were conducted to confirm the morphological and structural properties of the prepared materials.					
37169054	13	61	theme	reaction	1966:1973	arg1	kinetics					1975:1982	the Langmuir model and pseudo-second-order reaction kinetics	1923:1982	kinetics	1975:1982	The adsorption process of Fe(II) over the surface of prepared catalysts proceeded via the Langmuir model and pseudo-second-order reaction kinetics with R2 > 0.99.					
37169054	7	62	theme	morphological	991:1003	arg1	properties					1020:1029	the morphological and structural properties	987:1029	the morphological and structural properties of the prepared materials	987:1055	XRD, FTIR, SEM, TEM, BET, EDS, and TGA measurements were conducted to confirm the morphological and structural properties of the prepared materials.					
37169054	9	63	theme	synthesized	1270:1280	arg1	materials					1282:1290	the synthesized materials	1266:1290	the synthesized materials	1266:1290	The adsorption behavior toward Fe(II) as a pollutant for groundwater was studied for the synthesized materials.					
37169054	1	64	theme	heavy	173:177	arg1	metals					179:184	The highly poisonous, non-biodegradable heavy metals	133:184	The highly poisonous, non-biodegradable heavy metals	133:184	The highly poisonous, non-biodegradable heavy metals present serious concern in wastewater environmental sustainability and human health.					
37169054	10	65	theme	temperature	1385:1395	arg1	degrees					1397:1403	different temperature degrees	1375:1403	different temperature degrees (278, 288, 298, 308, and 318 K)	1375:1435	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	3	66	from	topic	458:462	arg1	field					471:475	the field	467:475	the field	467:475	Therefore, developing efficient and cost-effective adsorbents considers a significant and an emerging topic in the field the water purification.					
37169054	5	67	theme	simple	650:655	arg1	technique					668:676	The simple ultrasonic technique	646:676	The simple ultrasonic technique	646:676	The simple ultrasonic technique was used for doping ZnO nanoparticles into the Cs-g-PAN matrix to prepare chitosan-grafted polyacrylonitrile/ZnO (Cs-g-PAN/ZnO).					
37169054	3	68	theme	cost-effective	392:405	arg1	adsorbents					407:416	developing efficient and cost-effective adsorbents	367:416	developing efficient and cost-effective adsorbents	367:416	Therefore, developing efficient and cost-effective adsorbents considers a significant and an emerging topic in the field the water purification.					
37169054	3	69	dep	significant	430:440	arg1	a					428:428	a	428:428	a	428:428	Therefore, developing efficient and cost-effective adsorbents considers a significant and an emerging topic in the field the water purification.					
37169054	3	69	dep	significant	430:440	arg1	purification					487:498	the water purification	477:498	the water purification	477:498	Therefore, developing efficient and cost-effective adsorbents considers a significant and an emerging topic in the field the water purification.					
37169054	1	70	dep	poisonous	144:152	arg1	non-biodegradable					155:171	non-biodegradable	155:171	non-biodegradable	155:171	The highly poisonous, non-biodegradable heavy metals present serious concern in wastewater environmental sustainability and human health.					
37169054	11	71	theme	dosage	1637:1642	arg1	0.05 g/L					1644:1651	adsorbent dosage 0.05 g/L	1627:1651	adsorbent dosage 0.05 g/L	1627:1651	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	12	72	theme	adsorption	1767:1776	arg1	mechanism					1778:1786	the adsorption mechanism	1763:1786	the adsorption mechanism of Fe(II) ions	1763:1801	Langmuir and Freundlich's isothermal and kinetic studies have been analyzed to determine the adsorption mechanism of Fe(II) ions on the synthesized nanomaterials.					
37169054	5	73	theme	ultrasonic	657:666	arg1	technique					668:676	The simple ultrasonic technique	646:676	The simple ultrasonic technique	646:676	The simple ultrasonic technique was used for doping ZnO nanoparticles into the Cs-g-PAN matrix to prepare chitosan-grafted polyacrylonitrile/ZnO (Cs-g-PAN/ZnO).					
37169054	3	74	theme	efficient	378:386	arg1	adsorbents					407:416	developing efficient and cost-effective adsorbents	367:416	developing efficient and cost-effective adsorbents	367:416	Therefore, developing efficient and cost-effective adsorbents considers a significant and an emerging topic in the field the water purification.					
37169054	0	75	theme	ZnO	29:31	arg1	nano-composite					33:46	Chitosan-g-polyacrylonitrile ZnO nano-composite	0:46	Chitosan-g-polyacrylonitrile ZnO nano-composite	0:46	Chitosan-g-polyacrylonitrile ZnO nano-composite, synthesis and characterization as new and good adsorbent for Iron from groundwater.					
37169054	13	76	theme	catalysts	1899:1907	arg1	surface					1879:1885	the surface	1875:1885	the surface of prepared catalysts	1875:1907	The adsorption process of Fe(II) over the surface of prepared catalysts proceeded via the Langmuir model and pseudo-second-order reaction kinetics with R2 > 0.99.					
37169054	8	77	theme	specific	1083:1090	arg1	area					1100:1103	a specific surface area	1081:1103	a specific surface area of 20.23 m2/g	1081:1117	Cs-g-PAN/ZnO possesses a specific surface area of 20.23 m2/g with a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1.					
37169054	10	78	theme	pH	1307:1308	arg1	effect					1297:1302	The effect	1293:1302	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II)	1293:1463	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	8	79	contain	possesses	1071:1079	arg2	area					1100:1103	a specific surface area	1081:1103	a specific surface area of 20.23 m2/g	1081:1117	Cs-g-PAN/ZnO possesses a specific surface area of 20.23 m2/g with a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1.					
37169054	8	79	contain	possesses	1071:1079	arg1	Cs-g-PAN/ZnO					1058:1069	Cs-g-PAN/ZnO	1058:1069	Cs-g-PAN/ZnO	1058:1069	Cs-g-PAN/ZnO possesses a specific surface area of 20.23 m2/g with a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1.					
37169054	8	80	theme	20.23 m2/g	1108:1117	arg1	area					1100:1103	a specific surface area	1081:1103	a specific surface area of 20.23 m2/g	1081:1117	Cs-g-PAN/ZnO possesses a specific surface area of 20.23 m2/g with a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1.					
37169054	4	81	theme	graft	575:579	arg1	polymerization					581:594	graft polymerization	575:594	graft polymerization	575:594	Chitosan grafted polyacrylonitrile (Cs-g-PAN) was facially fabricated via graft polymerization using ammonium persulfate (APS) as the initiator.					
37169054	6	82	theme	pure	830:833	arg1	ZnO					835:837	pure ZnO	830:837	pure ZnO	830:837	For comparative study, pure ZnO and nanocomposite of PAN doped with ZnO (PAN/ZnO) were also prepared.					
37169054	10	83	dep	degrees	1397:1403	arg1	308					1421:1423	308	1421:1423	308	1421:1423	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	10	83	dep	degrees	1397:1403	arg1	318 K					1430:1434	318 K	1430:1434	318 K	1430:1434	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	12	84	theme	kinetic	1715:1721	arg1	studies					1723:1729	Langmuir and Freundlich's isothermal and kinetic studies	1674:1729	Langmuir and Freundlich's isothermal and kinetic studies	1674:1729	Langmuir and Freundlich's isothermal and kinetic studies have been analyzed to determine the adsorption mechanism of Fe(II) ions on the synthesized nanomaterials.					
37169054	10	85	theme	time	1325:1328	arg1	effect					1297:1302	The effect	1293:1302	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II)	1293:1463	The effect of pH (4-8), contact time (5-60 min), adsorbent dose (0.01-0.3 g), and different temperature degrees (278, 288, 298, 308, and 318 K) on the removal of iron (II) has been conducted.					
37169054	11	86	theme	time	1575:1578	arg1	30 min					1580:1585	contact time 30 min	1567:1585	contact time 30 min	1567:1585	The removal efficiency was achieved 100 % under the optimum condition, at pH = 7, contact time 30 min, adsorbate concentration 0.93 mg/L, and adsorbent dosage 0.05 g/L at room temperature.					
37169054	2	87	theme	water	349:353	arg1	kind					341:344	this kind	336:344	this kind of water	336:353	Using adsorption is an effective technology for the treatment of this kind of water.					
37169054	8	88	theme	31.58 nm	1139:1146	arg1	size					1131:1134	a pore size	1124:1134	a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1	1124:1178	Cs-g-PAN/ZnO possesses a specific surface area of 20.23 m2/g with a pore size of 31.58 nm and pore volume of 0.16 cm3 g-1.					
37169054	13	89	theme	Fe	1863:1864	arg1	process					1852:1858	The adsorption process	1837:1858	The adsorption process of Fe(II) over the surface of prepared catalysts	1837:1907	The adsorption process of Fe(II) over the surface of prepared catalysts proceeded via the Langmuir model and pseudo-second-order reaction kinetics with R2 > 0.99.					
36736136	9	0	theme	P	1538:1538	arg1	increase					1528:1535	a quadratic increase	1516:1535	a quadratic increase (P < 0.05) in crop LAB recovery	1516:1567	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	9	0	theme	P	1538:1538	arg1	<					1540:1540	P < 0.05	1538:1545	P < 0.05	1538:1545	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	12	1	theme	gut	1912:1914	arg1	microbiota					1916:1925	gut microbiota	1912:1925	gut microbiota	1912:1925	Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium.					
36736136	1	2	from	health	303:308	arg1	broilers					340:347	young broilers	334:347	young broilers	334:347	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	7	3	dep	increased	1280:1288	arg1	<					1293:1293	P < 0.01	1291:1298	P < 0.01	1291:1298	Feed conversion ratio increased (P < 0.01) linearly as GOS increased.					
36736136	7	3	dep	increased	1280:1288	arg1	increased					1317:1325	increased	1317:1325	increased (P < 0.01) linearly as GOS increased	1280:1325	Feed conversion ratio increased (P < 0.01) linearly as GOS increased.					
36736136	4	4	theme	cell	730:733	arg1	counts					735:740	complete blood cell counts	715:740	complete blood cell counts	715:740	Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were determined at 14 and 21 d.					
36736136	10	5	theme	blood	1672:1676	arg1	heterophil					1678:1687	blood heterophil	1672:1687	blood heterophil	1672:1687	At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased.					
36736136	6	6	theme	rRNA	1076:1079	arg1	sequencing					1091:1100	cecal microbiota via16S rRNA microbial sequencing	1052:1100	cecal microbiota via16S rRNA microbial sequencing	1052:1100	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	6	7	theme	bacteria	1006:1013	arg1	crop					1029:1032	crop	1029:1032	crop	1029:1032	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	6	7	theme	bacteria	1006:1013	arg1	pH					1044:1045	cecal pH	1038:1045	cecal pH	1038:1045	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	6	7	theme	bacteria	1006:1013	arg1	counts					1021:1026	crop presumptive lactic acid bacteria (LAB) counts	977:1026	crop presumptive lactic acid bacteria (LAB) counts	977:1026	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	9	8	theme	LAB	1556:1558	arg1	recovery					1560:1567	crop LAB recovery	1551:1567	crop LAB recovery	1551:1567	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	6	9	theme	microbiota	1058:1067	arg1	sequencing					1091:1100	cecal microbiota via16S rRNA microbial sequencing	1052:1100	cecal microbiota via16S rRNA microbial sequencing	1052:1100	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	6	10	theme	lactic	994:999	arg1	LAB					1016:1018	LAB	1016:1018	LAB	1016:1018	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	6	10	theme	lactic	994:999	arg1	bacteria					1006:1013	crop presumptive lactic acid bacteria	977:1013	crop presumptive lactic acid bacteria (LAB) counts	977:1026	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	0	11	theme	chicks	123:128	arg1	performance					47:57	performance	47:57	performance	47:57	Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks.					
36736136	0	11	theme	chicks	123:128	arg1	function					71:78	intestinal function	60:78	intestinal function	60:78	Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks.					
36736136	0	11	theme	chicks	123:128	arg1	composition					100:110	gut microbiota composition	85:110	gut microbiota composition	85:110	Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks.					
36736136	8	12	theme	dose-dependent	1439:1452	arg1	responses					1454:1462	dose-dependent responses	1439:1462	dose-dependent responses (P < 0.05)	1439:1473	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	8	12	theme	dose-dependent	1439:1452	arg1	<					1467:1467	P < 0.05	1465:1472	P < 0.05	1465:1472	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	6	13	dep	21	1136:1137	arg1	to					1133:1134	to	1133:1134	to	1133:1134	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	12	14	theme	beta	1876:1879	arg1	diversities					1881:1891	alpha and beta diversities	1866:1891	alpha and beta diversities	1866:1891	Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium.					
36736136	12	14	theme	beta	1876:1879	arg1	abundance					1942:1950	the abundance	1938:1950	the abundance of Ruminococcus and Bifidobacterium	1938:1986	Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium.					
36736136	6	15	theme	cecal	1052:1056	arg1	sequencing					1091:1100	cecal microbiota via16S rRNA microbial sequencing	1052:1100	cecal microbiota via16S rRNA microbial sequencing	1052:1100	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	9	16	theme	linear	1575:1580	arg1	P					1592:1592	P < 0.01	1592:1599	P < 0.01	1592:1599	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	9	16	theme	linear	1575:1580	arg1	decrease					1582:1589	a linear decrease	1573:1589	a linear decrease (P < 0.01) in ceca pH as GOS increased	1573:1628	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	1	17	theme	soy	210:212	arg1	GOS					239:241	GOS	239:241	GOS	239:241	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	1	17	theme	soy	210:212	arg1	raffinose					245:253	raffinose	245:253	raffinose	245:253	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	1	17	theme	soy	210:212	arg1	galactooligosaccharides					214:236	the dietary soy galactooligosaccharides	198:236	the dietary soy galactooligosaccharides (GOS)	198:242	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	1	17	theme	soy	210:212	arg1	stachyose					259:267	stachyose	259:267	stachyose	259:267	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	10	18	theme	dietary	1725:1731	arg1	GOS					1733:1735	dietary GOS	1725:1735	dietary GOS	1725:1735	At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased.					
36736136	12	19	theme	GOS	1851:1853	arg1	levels					1841:1846	Increasing levels	1830:1846	Increasing levels of GOS	1830:1853	Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium.					
36736136	6	20	theme	cecal	1038:1042	arg1	pH					1044:1045	cecal pH	1038:1045	cecal pH	1038:1045	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	6	20	theme	cecal	1038:1042	arg1	counts					1021:1026	crop presumptive lactic acid bacteria (LAB) counts	977:1026	crop presumptive lactic acid bacteria (LAB) counts	977:1026	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	9	21	theme	<	1594:1594	arg1	P					1592:1592	P < 0.01	1592:1599	P < 0.01	1592:1599	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	9	21	theme	<	1594:1594	arg1	decrease					1582:1589	a linear decrease	1573:1589	a linear decrease (P < 0.01) in ceca pH as GOS increased	1573:1628	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	12	22	theme	alpha	1866:1870	arg1	diversities					1881:1891	alpha and beta diversities	1866:1891	alpha and beta diversities	1866:1891	Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium.					
36736136	12	22	theme	alpha	1866:1870	arg1	abundance					1942:1950	the abundance	1938:1950	the abundance of Ruminococcus and Bifidobacterium	1938:1986	Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium.					
36736136	1	23	theme	systemic	315:322	arg1	stress					324:329	systemic stress	315:329	systemic stress	315:329	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	8	24	theme	N	1478:1478	arg1	retention					1480:1488	N retention	1478:1488	N retention	1478:1488	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	13	25	from	trial	2007:2011	arg1	Results					1989:1995	Results	1989:1995	Results from this trial	1989:2011	Results from this trial indicate that soy-derived GOS exert dose-dependent effects on nutrient utilization and intestinal health in young broilers.					
36736136	11	26	theme	<	1806:1806	arg1	P					1804:1804	P < 0.01	1804:1811	P < 0.01	1804:1811	Serum concentrations of FITC-d increased quadratically (P < 0.01) to dietary GOS.					
36736136	0	27	theme	microbiota	89:98	arg1	composition					100:110	gut microbiota composition	85:110	gut microbiota composition	85:110	Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks.					
36736136	5	28	theme	leakage	931:937	arg1	marker					917:922	a marker	915:922	a marker of gut leakage	915:937	At 21 d, 2 birds per cage were orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and serum samples were analyzed for FITC-d as a marker of gut leakage.					
36736136	5	28	theme	leakage	931:937	arg1	samples					879:885	serum samples	873:885	serum samples	873:885	At 21 d, 2 birds per cage were orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and serum samples were analyzed for FITC-d as a marker of gut leakage.					
36736136	6	29	dep	increased	1154:1162	arg1	increased					1199:1207	increased	1199:1207	increased	1199:1207	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	2	30	theme	corn	528:531	arg1	starch					533:538	corn starch	528:538	corn starch	528:538	Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch.					
36736136	2	31	theme	starch	533:538	arg1	expense					517:523	the expense	513:523	the expense of corn starch	513:538	Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch.					
36736136	4	32	theme	N	666:666	arg1	retention					668:676	N retention	666:676	N retention	666:676	Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were determined at 14 and 21 d.					
36736136	13	33	theme	nutrient	2075:2082	arg1	utilization					2084:2094	nutrient utilization	2075:2094	nutrient utilization	2075:2094	Results from this trial indicate that soy-derived GOS exert dose-dependent effects on nutrient utilization and intestinal health in young broilers.					
36736136	6	34	theme	feed	1142:1145	arg1	intake					1147:1152	feed intake	1142:1152	feed intake	1142:1152	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	5	35	theme	fluorescein	825:835	arg1	FITC-d					861:866	FITC-d	861:866	FITC-d	861:866	At 21 d, 2 birds per cage were orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and serum samples were analyzed for FITC-d as a marker of gut leakage.					
36736136	5	35	theme	fluorescein	825:835	arg1	isothiocyanate-dextran					837:858	fluorescein isothiocyanate-dextran	825:858	fluorescein isothiocyanate-dextran (FITC-d)	825:867	At 21 d, 2 birds per cage were orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and serum samples were analyzed for FITC-d as a marker of gut leakage.					
36736136	0	36	theme	Dietary	0:6	arg1	galactooligosaccharides					12:34	Dietary soy galactooligosaccharides	0:34	Dietary soy galactooligosaccharides	0:34	Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks.					
36736136	6	37	theme	microbial	1081:1089	arg1	sequencing					1091:1100	cecal microbiota via16S rRNA microbial sequencing	1052:1100	cecal microbiota via16S rRNA microbial sequencing	1052:1100	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	2	38	theme	protein	396:402	arg1	SPI					413:415	SPI	413:415	SPI	413:415	Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch.					
36736136	2	38	theme	protein	396:402	arg1	isolate					404:410	soy protein isolate	392:410	soy protein isolate (SPI)	392:416	Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch.					
36736136	3	39	dep	cages	594:598	arg1	replicate					584:592	replicate	584:592	replicate	584:592	These 5 treatments were administered to 10 replicate cages of 8 birds.					
36736136	11	40	theme	Serum	1748:1752	arg1	concentrations					1754:1767	Serum concentrations	1748:1767	Serum concentrations of FITC-d	1748:1777	Serum concentrations of FITC-d increased quadratically (P < 0.01) to dietary GOS.					
36736136	6	41	theme	dietary	1187:1193	arg1	GOS					1195:1197	dietary GOS	1187:1197	dietary GOS	1187:1197	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	1	42	theme	gastrointestinal	286:301	arg1	health					303:308	gastrointestinal health	286:308	gastrointestinal health	286:308	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	5	43	theme	serum	873:877	arg1	marker					917:922	a marker	915:922	a marker of gut leakage	915:937	At 21 d, 2 birds per cage were orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and serum samples were analyzed for FITC-d as a marker of gut leakage.					
36736136	5	43	theme	serum	873:877	arg1	samples					879:885	serum samples	873:885	serum samples	873:885	At 21 d, 2 birds per cage were orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and serum samples were analyzed for FITC-d as a marker of gut leakage.					
36736136	12	44	theme	Ruminococcus	1955:1966	arg1	abundance					1942:1950	the abundance	1938:1950	the abundance of Ruminococcus and Bifidobacterium	1938:1986	Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium.					
36736136	4	45	theme	complete	715:722	arg1	counts					735:740	complete blood cell counts	715:740	complete blood cell counts	715:740	Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were determined at 14 and 21 d.					
36736136	1	46	from	stress	324:329	arg1	broilers					340:347	young broilers	334:347	young broilers	334:347	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	12	47	theme	Bifidobacterium	1972:1986	arg1	abundance					1942:1950	the abundance	1938:1950	the abundance of Ruminococcus and Bifidobacterium	1938:1986	Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium.					
36736136	8	48	theme	excreta	1370:1376	arg1	moisture					1378:1385	excreta moisture	1370:1385	excreta moisture	1370:1385	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	1	49	theme	galactooligosaccharides	214:236	arg1	effects					187:193	the effects	183:193	the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers	183:347	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	7	50	theme	Feed	1258:1261	arg1	ratio					1274:1278	Feed conversion ratio	1258:1278	Feed conversion ratio	1258:1278	Feed conversion ratio increased (P < 0.01) linearly as GOS increased.					
36736136	6	51	theme	intestinal	954:963	arg1	morphology					965:974	intestinal morphology	954:974	intestinal morphology	954:974	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	9	52	theme	quadratic	1518:1526	arg1	increase					1528:1535	a quadratic increase	1516:1535	a quadratic increase (P < 0.05) in crop LAB recovery	1516:1567	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	9	52	theme	quadratic	1518:1526	arg1	<					1540:1540	P < 0.05	1538:1545	P < 0.05	1538:1545	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	8	53	theme	linear	1339:1344	arg1	increases					1346:1354	linear increases	1339:1354	linear increases (P < 0.05)	1339:1365	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	8	53	theme	linear	1339:1344	arg1	<					1359:1359	P < 0.05	1357:1364	P < 0.05	1357:1364	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	4	54	theme	metabolizeable	688:701	arg1	energy					703:708	apparent metabolizeable energy	679:708	apparent metabolizeable energy	679:708	Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were determined at 14 and 21 d.					
36736136	6	55	theme	P	1174:1174	arg1	<					1176:1176	P < 0.01	1174:1181	P < 0.01	1174:1181	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	6	56	theme	P	1233:1233	arg1	<					1235:1235	P < 0.05	1233:1240	P < 0.05	1233:1240	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	2	57	theme	4:1	506:508	arg1	ratio					497:501	a ratio	495:501	a ratio of 4:1 at the expense of corn starch	495:538	Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch.					
36736136	10	58	theme	P	1659:1659	arg1	increase					1649:1656	a linear increase	1640:1656	a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio	1640:1707	At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased.					
36736136	10	58	theme	P	1659:1659	arg1	<					1661:1661	P < 0.05	1659:1666	P < 0.05	1659:1666	At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased.					
36736136	13	59	theme	young	2121:2125	arg1	broilers					2127:2134	young broilers	2121:2134	young broilers	2121:2134	Results from this trial indicate that soy-derived GOS exert dose-dependent effects on nutrient utilization and intestinal health in young broilers.					
36736136	11	60	theme	dietary	1817:1823	arg1	GOS					1825:1827	dietary GOS	1817:1827	dietary GOS	1817:1827	Serum concentrations of FITC-d increased quadratically (P < 0.01) to dietary GOS.					
36736136	12	61	theme	microbiota	1916:1925	arg1	diversities					1881:1891	alpha and beta diversities	1866:1891	alpha and beta diversities	1866:1891	Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium.					
36736136	12	61	theme	microbiota	1916:1925	arg1	abundance					1942:1950	the abundance	1938:1950	the abundance of Ruminococcus and Bifidobacterium	1938:1986	Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium.					
36736136	12	61	theme	microbiota	1916:1925	arg1	composition					1897:1907	composition	1897:1907	composition	1897:1907	Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium.					
36736136	13	62	link	soy-derived	2027:2037	arg1	GOS					2039:2041	soy-derived GOS	2027:2041	soy-derived GOS	2027:2041	Results from this trial indicate that soy-derived GOS exert dose-dependent effects on nutrient utilization and intestinal health in young broilers.					
36736136	1	63	from	performance	273:283	arg1	broilers					340:347	young broilers	334:347	young broilers	334:347	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	2	64	dep	added	462:466	arg1	%					460:460	0.9, 1.8, 2.7, or 3.6%	439:460	0.9, 1.8, 2.7, or 3.6%	439:460	Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch.					
36736136	4	65	theme	blood	724:728	arg1	counts					735:740	complete blood cell counts	715:740	complete blood cell counts	715:740	Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were determined at 14 and 21 d.					
36736136	9	66	theme	crop	1551:1554	arg1	recovery					1560:1567	crop LAB recovery	1551:1567	crop LAB recovery	1551:1567	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	3	67	theme	birds	605:609	arg1	cages					594:598	10 replicate cages	581:598	10 replicate cages of 8 birds	581:609	These 5 treatments were administered to 10 replicate cages of 8 birds.					
36736136	6	68	theme	via16S	1069:1074	arg1	sequencing					1091:1100	cecal microbiota via16S rRNA microbial sequencing	1052:1100	cecal microbiota via16S rRNA microbial sequencing	1052:1100	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	13	69	theme	intestinal	2100:2109	arg1	health					2111:2116	intestinal health	2100:2116	intestinal health	2100:2116	Results from this trial indicate that soy-derived GOS exert dose-dependent effects on nutrient utilization and intestinal health in young broilers.					
36736136	6	70	theme	acid	1001:1004	arg1	LAB					1016:1018	LAB	1016:1018	LAB	1016:1018	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	6	70	theme	acid	1001:1004	arg1	bacteria					1006:1013	crop presumptive lactic acid bacteria	977:1013	crop presumptive lactic acid bacteria (LAB) counts	977:1026	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	8	71	from	responses	1454:1462	arg1	moisture					1378:1385	excreta moisture	1370:1385	excreta moisture	1370:1385	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	9	72	dep	ceca	1605:1608	arg1	pH					1610:1611	pH	1610:1611	pH	1610:1611	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	9	73	from	increase	1528:1535	arg1	recovery					1560:1567	crop LAB recovery	1551:1567	crop LAB recovery	1551:1567	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	9	73	from	increase	1528:1535	arg1	ceca					1605:1608	ceca	1605:1608	ceca pH as GOS increased	1605:1628	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	2	74	theme	added	462:466	arg1	stachyose					468:476	0.9, 1.8, 2.7, or 3.6% added stachyose	439:476	0.9, 1.8, 2.7, or 3.6% added stachyose	439:476	Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch.					
36736136	6	75	theme	presumptive	982:992	arg1	LAB					1016:1018	LAB	1016:1018	LAB	1016:1018	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	6	75	theme	presumptive	982:992	arg1	bacteria					1006:1013	crop presumptive lactic acid bacteria	977:1013	crop presumptive lactic acid bacteria (LAB) counts	977:1026	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	10	76	theme	lymphocyte	1692:1701	arg1	ratio					1703:1707	lymphocyte ratio	1692:1707	lymphocyte ratio	1692:1707	At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased.					
36736136	8	77	from	increases	1346:1354	arg1	moisture					1378:1385	excreta moisture	1370:1385	excreta moisture	1370:1385	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	1	78	theme	dietary	202:208	arg1	GOS					239:241	GOS	239:241	GOS	239:241	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	1	78	theme	dietary	202:208	arg1	raffinose					245:253	raffinose	245:253	raffinose	245:253	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	1	78	theme	dietary	202:208	arg1	galactooligosaccharides					214:236	the dietary soy galactooligosaccharides	198:236	the dietary soy galactooligosaccharides (GOS)	198:242	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	1	78	theme	dietary	202:208	arg1	stachyose					259:267	stachyose	259:267	stachyose	259:267	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	5	79	theme	gut	927:929	arg1	leakage					931:937	gut leakage	927:937	gut leakage	927:937	At 21 d, 2 birds per cage were orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and serum samples were analyzed for FITC-d as a marker of gut leakage.					
36736136	10	80	located	observed	1713:1720	arg2	<					1661:1661	P < 0.05	1659:1666	P < 0.05	1659:1666	At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased.					
36736136	10	80	located	observed	1713:1720	arg2	increase					1649:1656	a linear increase	1640:1656	a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio	1640:1707	At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased.					
36736136	10	80	located	observed	1713:1720	arg1	d					1637:1637	14 d	1634:1637	14 d	1634:1637	At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased.					
36736136	13	81	theme	soy-derived	2027:2037	arg1	GOS					2039:2041	soy-derived GOS	2027:2041	soy-derived GOS	2027:2041	Results from this trial indicate that soy-derived GOS exert dose-dependent effects on nutrient utilization and intestinal health in young broilers.					
36736136	8	82	theme	P	1465:1465	arg1	responses					1454:1462	dose-dependent responses	1439:1462	dose-dependent responses (P < 0.05)	1439:1473	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	8	82	theme	P	1465:1465	arg1	<					1467:1467	P < 0.05	1465:1472	P < 0.05	1465:1472	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	1	83	from	effects	187:193	arg1	performance					273:283	performance	273:283	performance	273:283	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	1	83	from	effects	187:193	arg1	health					303:308	gastrointestinal health	286:308	gastrointestinal health	286:308	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	1	83	from	effects	187:193	arg1	stress					324:329	systemic stress	315:329	systemic stress	315:329	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	12	84	theme	Increasing	1830:1839	arg1	levels					1841:1846	Increasing levels	1830:1846	Increasing levels of GOS	1830:1853	Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium.					
36736136	0	85	theme	gut	85:87	arg1	composition					100:110	gut microbiota composition	85:110	gut microbiota composition	85:110	Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks.					
36736136	1	86	theme	experiment	153:162	arg1	objective					135:143	The objective	131:143	The objective of this experiment	131:162	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	11	87	theme	FITC-d	1772:1777	arg1	concentrations					1754:1767	Serum concentrations	1748:1767	Serum concentrations of FITC-d	1748:1777	Serum concentrations of FITC-d increased quadratically (P < 0.01) to dietary GOS.					
36736136	1	88	theme	young	334:338	arg1	broilers					340:347	young broilers	334:347	young broilers	334:347	The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers.					
36736136	6	89	dep	linearly	1164:1171	arg1	<					1176:1176	P < 0.01	1174:1181	P < 0.01	1174:1181	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	4	90	theme	excreta	648:654	arg1	moisture					656:663	excreta moisture	648:663	excreta moisture	648:663	Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were determined at 14 and 21 d.					
36736136	0	91	theme	growing	115:121	arg1	chicks					123:128	growing chicks	115:128	growing chicks	115:128	Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks.					
36736136	13	92	theme	dose-dependent	2049:2062	arg1	effects					2064:2070	dose-dependent effects	2049:2070	dose-dependent effects	2049:2070	Results from this trial indicate that soy-derived GOS exert dose-dependent effects on nutrient utilization and intestinal health in young broilers.					
36736136	2	93	theme	GOS-devoid	367:376	arg1	diet					378:381	a GOS-devoid diet	365:381	a GOS-devoid diet based on soy protein isolate (SPI)	365:416	Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch.					
36736136	0	94	theme	soy	8:10	arg1	galactooligosaccharides					12:34	Dietary soy galactooligosaccharides	0:34	Dietary soy galactooligosaccharides	0:34	Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks.					
36736136	10	95	from	increase	1649:1656	arg1	heterophil					1678:1687	blood heterophil	1672:1687	blood heterophil	1672:1687	At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased.					
36736136	2	96	theme	soy	392:394	arg1	SPI					413:415	SPI	413:415	SPI	413:415	Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch.					
36736136	2	96	theme	soy	392:394	arg1	isolate					404:410	soy protein isolate	392:410	soy protein isolate (SPI)	392:416	Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch.					
36736136	9	97	from	decrease	1582:1589	arg1	recovery					1560:1567	crop LAB recovery	1551:1567	crop LAB recovery	1551:1567	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	9	97	from	decrease	1582:1589	arg1	ceca					1605:1608	ceca	1605:1608	ceca pH as GOS increased	1605:1628	There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased.					
36736136	2	98	theme	SPI	425:427	arg1	diet					429:432	the SPI diet	421:432	the SPI diet	421:432	Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch.					
36736136	2	99	from	expense	517:523	arg1	ratio					497:501	a ratio	495:501	a ratio of 4:1 at the expense of corn starch	495:538	Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch.					
36736136	11	100	dep	quadratically	1789:1801	arg1	P					1804:1804	P < 0.01	1804:1811	P < 0.01	1804:1811	Serum concentrations of FITC-d increased quadratically (P < 0.01) to dietary GOS.					
36736136	0	101	theme	intestinal	60:69	arg1	function					71:78	intestinal function	60:78	intestinal function	60:78	Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks.					
36736136	7	102	theme	P	1291:1291	arg1	<					1293:1293	P < 0.01	1291:1298	P < 0.01	1291:1298	Feed conversion ratio increased (P < 0.01) linearly as GOS increased.					
36736136	0	103	dep	performance	47:57	arg1	the					43:45	the	43:45	the	43:45	Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks.					
36736136	6	104	dep	increased	1222:1230	arg1	<					1235:1235	P < 0.05	1233:1240	P < 0.05	1233:1240	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	6	105	theme	crop	977:980	arg1	LAB					1016:1018	LAB	1016:1018	LAB	1016:1018	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	6	105	theme	crop	977:980	arg1	bacteria					1006:1013	crop presumptive lactic acid bacteria	977:1013	crop presumptive lactic acid bacteria (LAB) counts	977:1026	Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically.					
36736136	10	106	theme	linear	1642:1647	arg1	increase					1649:1656	a linear increase	1640:1656	a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio	1640:1707	At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased.					
36736136	10	106	theme	linear	1642:1647	arg1	<					1661:1661	P < 0.05	1659:1666	P < 0.05	1659:1666	At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased.					
36736136	8	107	theme	dietary	1390:1396	arg1	GOS					1398:1400	dietary GOS	1390:1400	dietary GOS	1390:1400	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	7	108	theme	conversion	1263:1272	arg1	ratio					1274:1278	Feed conversion ratio	1258:1278	Feed conversion ratio	1258:1278	Feed conversion ratio increased (P < 0.01) linearly as GOS increased.					
36736136	8	109	theme	P	1357:1357	arg1	increases					1346:1354	linear increases	1339:1354	linear increases (P < 0.05)	1339:1365	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	8	109	theme	P	1357:1357	arg1	<					1359:1359	P < 0.05	1357:1364	P < 0.05	1357:1364	There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn.					
36736136	4	110	theme	apparent	679:686	arg1	energy					703:708	apparent metabolizeable energy	679:708	apparent metabolizeable energy	679:708	Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were determined at 14 and 21 d.					
36716844	9	0	theme	phenolic	1306:1313	arg1	content					1315:1321	total phenolic content	1300:1321	total phenolic content of the fruit	1300:1334	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	4	1	from	chitosan	458:465	arg1	work					475:478	this work to create a novel antioxidant and biodegradable food packaging film	470:546	this work to create a novel antioxidant and biodegradable food packaging film	470:546	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	7	2	theme	water	919:923	arg1	permeability					931:942	the water vapor permeability	915:942	the water vapor permeability	915:942	Moreover, KC addition had a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film.					
36716844	7	3	from	impact	905:910	arg1	permeability					931:942	the water vapor permeability	915:942	the water vapor permeability	915:942	Moreover, KC addition had a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film.					
36716844	7	3	from	impact	905:910	arg1	biodegradability					952:967	the biodegradability	948:967	the biodegradability of the chitosan film	948:988	Moreover, KC addition had a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film.					
36716844	7	4	theme	film	985:988	arg1	permeability					931:942	the water vapor permeability	915:942	the water vapor permeability	915:942	Moreover, KC addition had a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film.					
36716844	7	4	theme	film	985:988	arg1	biodegradability					952:967	the biodegradability	948:967	the biodegradability of the chitosan film	948:988	Moreover, KC addition had a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film.					
36716844	4	5	theme	biodegradable	514:526	arg1	film					543:546	a novel antioxidant and biodegradable food packaging film	490:546	a novel antioxidant and biodegradable food packaging film	490:546	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	8	6	theme	strong	1075:1080	arg1	barrier					1082:1088	strong barrier	1075:1088	strong barrier	1075:1088	Because of the synergistic action of FLE and KC, the Chitosan/KC/FLE film delivered strong barrier and antioxidant capabilities.					
36716844	6	7	theme	moisture	762:769	arg1	characteristics					779:793	the light and moisture barrier characteristics	748:793	the light and moisture barrier characteristics	748:793	The addition of FLE and/or KC significantly improved the light and moisture barrier characteristics, mechanical properties, and antioxidant capabilities of chitosan film.					
36716844	6	8	theme	light	752:756	arg1	characteristics					779:793	the light and moisture barrier characteristics	748:793	the light and moisture barrier characteristics	748:793	The addition of FLE and/or KC significantly improved the light and moisture barrier characteristics, mechanical properties, and antioxidant capabilities of chitosan film.					
36716844	9	9	theme	apple	1200:1204	arg1	slices					1206:1211	fresh-cut apple slices	1190:1211	fresh-cut apple slices	1190:1211	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	10	10	theme	Chitosan/KC/FLE	1364:1378	arg1	film					1380:1383	Chitosan/KC/FLE film	1364:1383	Chitosan/KC/FLE film	1364:1383	According to our findings, Chitosan/KC/FLE film might be employed as a possible food packaging material in the food industry.					
36716844	10	10	theme	Chitosan/KC/FLE	1364:1378	arg1	material					1432:1439	a possible food packaging material	1406:1439	a possible food packaging material in the food industry	1406:1460	According to our findings, Chitosan/KC/FLE film might be employed as a possible food packaging material in the food industry.					
36716844	5	11	theme	physical	661:668	arg1	aspects					686:692	structural, physical, and functional aspects	649:692	structural, physical, and functional aspects	649:692	Chitosan/KC/FLE film was compared to chitosan film, Chitosan/KC, and Chitosan/FLE films in terms of structural, physical, and functional aspects.					
36716844	6	12	theme	chitosan	851:858	arg1	capabilities					835:846	antioxidant capabilities	823:846	antioxidant capabilities	823:846	The addition of FLE and/or KC significantly improved the light and moisture barrier characteristics, mechanical properties, and antioxidant capabilities of chitosan film.					
36716844	6	12	theme	chitosan	851:858	arg1	characteristics					779:793	the light and moisture barrier characteristics	748:793	the light and moisture barrier characteristics	748:793	The addition of FLE and/or KC significantly improved the light and moisture barrier characteristics, mechanical properties, and antioxidant capabilities of chitosan film.					
36716844	6	12	theme	chitosan	851:858	arg1	properties					807:816	mechanical properties	796:816	mechanical properties	796:816	The addition of FLE and/or KC significantly improved the light and moisture barrier characteristics, mechanical properties, and antioxidant capabilities of chitosan film.					
36716844	5	13	theme	Chitosan/KC/FLE	549:563	arg1	film					565:568	Chitosan/KC/FLE film	549:568	Chitosan/KC/FLE film	549:568	Chitosan/KC/FLE film was compared to chitosan film, Chitosan/KC, and Chitosan/FLE films in terms of structural, physical, and functional aspects.					
36716844	4	14	theme	packaging	533:541	arg1	film					543:546	a novel antioxidant and biodegradable food packaging film	490:546	a novel antioxidant and biodegradable food packaging film	490:546	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	9	15	theme	weight	1267:1272	arg1	loss					1274:1277	the weight loss	1263:1277	the weight loss	1263:1277	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	4	16	theme	Natural	382:388	arg1	KC					406:407	KC	406:407	KC	406:407	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	4	16	theme	Natural	382:388	arg1	clay					400:403	Natural kaolinite clay	382:403	Natural kaolinite clay (KC)	382:408	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	4	17	theme	food	528:531	arg1	film					543:546	a novel antioxidant and biodegradable food packaging film	490:546	a novel antioxidant and biodegradable food packaging film	490:546	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	10	18	theme	food	1417:1420	arg1	film					1380:1383	Chitosan/KC/FLE film	1364:1383	Chitosan/KC/FLE film	1364:1383	According to our findings, Chitosan/KC/FLE film might be employed as a possible food packaging material in the food industry.					
36716844	10	18	theme	food	1417:1420	arg1	material					1432:1439	a possible food packaging material	1406:1439	a possible food packaging material in the food industry	1406:1460	According to our findings, Chitosan/KC/FLE film might be employed as a possible food packaging material in the food industry.					
36716844	7	19	theme	remarkable	894:903	arg1	impact					905:910	a remarkable impact	892:910	a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film	892:988	Moreover, KC addition had a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film.					
36716844	5	20	theme	functional	675:684	arg1	aspects					686:692	structural, physical, and functional aspects	649:692	structural, physical, and functional aspects	649:692	Chitosan/KC/FLE film was compared to chitosan film, Chitosan/KC, and Chitosan/FLE films in terms of structural, physical, and functional aspects.					
36716844	6	21	theme	antioxidant	823:833	arg1	capabilities					835:846	antioxidant capabilities	823:846	antioxidant capabilities	823:846	The addition of FLE and/or KC significantly improved the light and moisture barrier characteristics, mechanical properties, and antioxidant capabilities of chitosan film.					
36716844	9	22	theme	good	1230:1233	arg1	preservation					1240:1251	good food preservation	1230:1251	good food preservation	1230:1251	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	6	23	theme	KC	722:723	arg1	addition					699:706	The addition	695:706	The addition of FLE and/or KC	695:723	The addition of FLE and/or KC significantly improved the light and moisture barrier characteristics, mechanical properties, and antioxidant capabilities of chitosan film.					
36716844	9	24	theme	fresh-cut	1190:1198	arg1	slices					1206:1211	fresh-cut apple slices	1190:1211	fresh-cut apple slices	1190:1211	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	0	25	theme	packaging	5:13	arg1	film					25:28	Food packaging composite film	0:28	Food packaging composite film	0:28	Food packaging composite film based on chitosan, natural kaolinite clay, and Ficus.					
36716844	9	26	theme	browning	1280:1287	arg1	index					1289:1293	browning index	1280:1293	browning index	1280:1293	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	8	27	theme	synergistic	1006:1016	arg1	action					1018:1023	the synergistic action	1002:1023	the synergistic action of FLE and KC	1002:1037	Because of the synergistic action of FLE and KC, the Chitosan/KC/FLE film delivered strong barrier and antioxidant capabilities.					
36716844	5	28	theme	aspects	686:692	arg1	terms					640:644	terms	640:644	terms of structural, physical, and functional aspects	640:692	Chitosan/KC/FLE film was compared to chitosan film, Chitosan/KC, and Chitosan/FLE films in terms of structural, physical, and functional aspects.					
36716844	5	29	theme	Chitosan/FLE	618:629	arg1	films					631:635	Chitosan/FLE films	618:635	Chitosan/FLE films in terms of structural, physical, and functional aspects	618:692	Chitosan/KC/FLE film was compared to chitosan film, Chitosan/KC, and Chitosan/FLE films in terms of structural, physical, and functional aspects.					
36716844	0	30	theme	Food	0:3	arg1	film					25:28	Food packaging composite film	0:28	Food packaging composite film	0:28	Food packaging composite film based on chitosan, natural kaolinite clay, and Ficus.					
36716844	8	31	theme	KC	1036:1037	arg1	action					1018:1023	the synergistic action	1002:1023	the synergistic action of FLE and KC	1002:1037	Because of the synergistic action of FLE and KC, the Chitosan/KC/FLE film delivered strong barrier and antioxidant capabilities.					
36716844	5	32	theme	chitosan	586:593	arg1	film					595:598	chitosan film	586:598	chitosan film	586:598	Chitosan/KC/FLE film was compared to chitosan film, Chitosan/KC, and Chitosan/FLE films in terms of structural, physical, and functional aspects.					
36716844	6	33	theme	FLE	711:713	arg1	addition					699:706	The addition	695:706	The addition of FLE and/or KC	695:723	The addition of FLE and/or KC significantly improved the light and moisture barrier characteristics, mechanical properties, and antioxidant capabilities of chitosan film.					
36716844	7	34	contain	had	888:890	arg1	addition					879:886	KC addition	876:886	KC addition	876:886	Moreover, KC addition had a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film.					
36716844	7	34	contain	had	888:890	arg2	impact					905:910	a remarkable impact	892:910	a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film	892:988	Moreover, KC addition had a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film.					
36716844	10	35	from	material	1432:1439	arg1	industry					1453:1460	the food industry	1444:1460	the food industry	1444:1460	According to our findings, Chitosan/KC/FLE film might be employed as a possible food packaging material in the food industry.					
36716844	7	36	theme	KC	876:877	arg1	addition					879:886	KC addition	876:886	KC addition	876:886	Moreover, KC addition had a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film.					
36716844	0	37	theme	composite	15:23	arg1	film					25:28	Food packaging composite film	0:28	Food packaging composite film	0:28	Food packaging composite film based on chitosan, natural kaolinite clay, and Ficus.					
36716844	4	38	theme	antioxidant	498:508	arg1	film					543:546	a novel antioxidant and biodegradable food packaging film	490:546	a novel antioxidant and biodegradable food packaging film	490:546	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	2	39	theme	environmental	162:174	arg1	contamination					184:196	environmental plastic contamination	162:196	environmental plastic contamination	162:196	The problem of environmental plastic contamination is one of the most serious issues facing our world today.					
36716844	9	40	theme	Chitosan/KC/FLE	1133:1147	arg1	material					1178:1185	packaging material	1168:1185	packaging material	1168:1185	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	9	40	theme	Chitosan/KC/FLE	1133:1147	arg1	film					1149:1152	Chitosan/KC/FLE film	1133:1152	Chitosan/KC/FLE film	1133:1152	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	4	41	theme	novel	492:496	arg1	film					543:546	a novel antioxidant and biodegradable food packaging film	490:546	a novel antioxidant and biodegradable food packaging film	490:546	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	5	42	theme	structural	649:658	arg1	aspects					686:692	structural, physical, and functional aspects	649:692	structural, physical, and functional aspects	649:692	Chitosan/KC/FLE film was compared to chitosan film, Chitosan/KC, and Chitosan/FLE films in terms of structural, physical, and functional aspects.					
36716844	2	43	theme	serious	217:223	arg1	issues					225:230	the most serious issues	208:230	the most serious issues facing our world today	208:253	The problem of environmental plastic contamination is one of the most serious issues facing our world today.					
36716844	5	44	from	Chitosan/KC	601:611	arg1	terms					640:644	terms	640:644	terms of structural, physical, and functional aspects	640:692	Chitosan/KC/FLE film was compared to chitosan film, Chitosan/KC, and Chitosan/FLE films in terms of structural, physical, and functional aspects.					
36716844	8	45	theme	FLE	1028:1030	arg1	action					1018:1023	the synergistic action	1002:1023	the synergistic action of FLE and KC	1002:1037	Because of the synergistic action of FLE and KC, the Chitosan/KC/FLE film delivered strong barrier and antioxidant capabilities.					
36716844	4	46	theme	leaf	420:423	arg1	FLE					434:436	FLE	434:436	FLE	434:436	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	4	46	theme	leaf	420:423	arg1	extract					425:431	Ficus leaf extract	414:431	Ficus leaf extract (FLE)	414:437	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	10	47	theme	possible	1408:1415	arg1	film					1380:1383	Chitosan/KC/FLE film	1364:1383	Chitosan/KC/FLE film	1364:1383	According to our findings, Chitosan/KC/FLE film might be employed as a possible food packaging material in the food industry.					
36716844	10	47	theme	possible	1408:1415	arg1	material					1432:1439	a possible food packaging material	1406:1439	a possible food packaging material in the food industry	1406:1460	According to our findings, Chitosan/KC/FLE film might be employed as a possible food packaging material in the food industry.					
36716844	3	48	theme	environmental	361:373	arg1	issue					375:379	an environmental issue	358:379	an environmental issue	358:379	The majority of the packaging materials used to preserve food are made of plastic which is considered an environmental issue.					
36716844	8	49	theme	antioxidant	1094:1104	arg1	capabilities					1106:1117	antioxidant capabilities	1094:1117	antioxidant capabilities	1094:1117	Because of the synergistic action of FLE and KC, the Chitosan/KC/FLE film delivered strong barrier and antioxidant capabilities.					
36716844	3	50	theme	packaging	276:284	arg1	materials					286:294	the packaging materials	272:294	the packaging materials used to preserve food	272:316	The majority of the packaging materials used to preserve food are made of plastic which is considered an environmental issue.					
36716844	4	51	theme	Ficus	414:418	arg1	FLE					434:436	FLE	434:436	FLE	434:436	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	4	51	theme	Ficus	414:418	arg1	extract					425:431	Ficus leaf extract	414:431	Ficus leaf extract (FLE)	414:437	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	10	52	theme	packaging	1422:1430	arg1	film					1380:1383	Chitosan/KC/FLE film	1364:1383	Chitosan/KC/FLE film	1364:1383	According to our findings, Chitosan/KC/FLE film might be employed as a possible food packaging material in the food industry.					
36716844	10	52	theme	packaging	1422:1430	arg1	material					1432:1439	a possible food packaging material	1406:1439	a possible food packaging material in the food industry	1406:1460	According to our findings, Chitosan/KC/FLE film might be employed as a possible food packaging material in the food industry.					
36716844	5	53	from	film	595:598	arg1	terms					640:644	terms	640:644	terms of structural, physical, and functional aspects	640:692	Chitosan/KC/FLE film was compared to chitosan film, Chitosan/KC, and Chitosan/FLE films in terms of structural, physical, and functional aspects.					
36716844	3	54	theme	materials	286:294	arg1	majority					260:267	The majority	256:267	The majority of the packaging materials used to preserve food	256:316	The majority of the packaging materials used to preserve food are made of plastic which is considered an environmental issue.					
36716844	6	55	theme	barrier	771:777	arg1	characteristics					779:793	the light and moisture barrier characteristics	748:793	the light and moisture barrier characteristics	748:793	The addition of FLE and/or KC significantly improved the light and moisture barrier characteristics, mechanical properties, and antioxidant capabilities of chitosan film.					
36716844	6	56	theme	mechanical	796:805	arg1	properties					807:816	mechanical properties	796:816	mechanical properties	796:816	The addition of FLE and/or KC significantly improved the light and moisture barrier characteristics, mechanical properties, and antioxidant capabilities of chitosan film.					
36716844	9	57	theme	packaging	1168:1176	arg1	material					1178:1185	packaging material	1168:1185	packaging material	1168:1185	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	9	57	theme	packaging	1168:1176	arg1	film					1149:1152	Chitosan/KC/FLE film	1133:1152	Chitosan/KC/FLE film	1133:1152	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	8	58	theme	Chitosan/KC/FLE	1044:1058	arg1	film					1060:1063	the Chitosan/KC/FLE film	1040:1063	the Chitosan/KC/FLE film	1040:1063	Because of the synergistic action of FLE and KC, the Chitosan/KC/FLE film delivered strong barrier and antioxidant capabilities.					
36716844	4	59	theme	kaolinite	390:398	arg1	KC					406:407	KC	406:407	KC	406:407	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	4	59	theme	kaolinite	390:398	arg1	clay					400:403	Natural kaolinite clay	382:403	Natural kaolinite clay (KC)	382:408	Natural kaolinite clay (KC) and Ficus leaf extract (FLE) were combined with chitosan in this work to create a novel antioxidant and biodegradable food packaging film.					
36716844	2	60	theme	world	243:247	arg1	today					249:253	our world today	239:253	our world today	239:253	The problem of environmental plastic contamination is one of the most serious issues facing our world today.					
36716844	9	61	theme	fruit	1330:1334	arg1	loss					1274:1277	the weight loss	1263:1277	the weight loss	1263:1277	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	9	61	theme	fruit	1330:1334	arg1	index					1289:1293	browning index	1280:1293	browning index	1280:1293	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	9	61	theme	fruit	1330:1334	arg1	content					1315:1321	total phenolic content	1300:1321	total phenolic content of the fruit	1300:1334	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	5	62	from	films	631:635	arg1	terms					640:644	terms	640:644	terms of structural, physical, and functional aspects	640:692	Chitosan/KC/FLE film was compared to chitosan film, Chitosan/KC, and Chitosan/FLE films in terms of structural, physical, and functional aspects.					
36716844	7	63	theme	chitosan	976:983	arg1	film					985:988	the chitosan film	972:988	the chitosan film	972:988	Moreover, KC addition had a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film.					
36716844	2	64	theme	contamination	184:196	arg1	problem					151:157	The problem	147:157	The problem of environmental plastic contamination	147:196	The problem of environmental plastic contamination is one of the most serious issues facing our world today.					
36716844	2	64	theme	contamination	184:196	arg1	issues					225:230	the most serious issues	208:230	the most serious issues facing our world today	208:253	The problem of environmental plastic contamination is one of the most serious issues facing our world today.					
36716844	2	64	theme	contamination	184:196	arg1	one					201:203	one	201:203	one	201:203	The problem of environmental plastic contamination is one of the most serious issues facing our world today.					
36716844	7	65	theme	vapor	925:929	arg1	permeability					931:942	the water vapor permeability	915:942	the water vapor permeability	915:942	Moreover, KC addition had a remarkable impact on the water vapor permeability and the biodegradability of the chitosan film.					
36716844	1	66	theme	fresh-cut	110:118	arg1	preservation					133:144	fresh-cut apple slices preservation	110:144	fresh-cut apple slices preservation	110:144	carica leaves extract for fresh-cut apple slices preservation.					
36716844	0	67	theme	kaolinite	57:65	arg1	clay					67:70	natural kaolinite clay	49:70	natural kaolinite clay	49:70	Food packaging composite film based on chitosan, natural kaolinite clay, and Ficus.					
36716844	2	68	theme	plastic	176:182	arg1	contamination					184:196	environmental plastic contamination	162:196	environmental plastic contamination	162:196	The problem of environmental plastic contamination is one of the most serious issues facing our world today.					
36716844	1	69	theme	apple	120:124	arg1	preservation					133:144	fresh-cut apple slices preservation	110:144	fresh-cut apple slices preservation	110:144	carica leaves extract for fresh-cut apple slices preservation.					
36716844	9	70	theme	total	1300:1304	arg1	content					1315:1321	total phenolic content	1300:1321	total phenolic content of the fruit	1300:1334	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	0	71	theme	natural	49:55	arg1	clay					67:70	natural kaolinite clay	49:70	natural kaolinite clay	49:70	Food packaging composite film based on chitosan, natural kaolinite clay, and Ficus.					
36716844	9	72	theme	food	1235:1238	arg1	preservation					1240:1251	good food preservation	1230:1251	good food preservation	1230:1251	Furthermore, Chitosan/KC/FLE film was tested as packaging material on fresh-cut apple slices and demonstrated good food preservation regarding the weight loss, browning index, and total phenolic content of the fruit.					
36716844	2	73	theme	issues	225:230	arg1	problem					151:157	The problem	147:157	The problem of environmental plastic contamination	147:196	The problem of environmental plastic contamination is one of the most serious issues facing our world today.					
36716844	2	73	theme	issues	225:230	arg1	issues					225:230	the most serious issues	208:230	the most serious issues facing our world today	208:253	The problem of environmental plastic contamination is one of the most serious issues facing our world today.					
36716844	2	73	theme	issues	225:230	arg1	one					201:203	one	201:203	one	201:203	The problem of environmental plastic contamination is one of the most serious issues facing our world today.					
36716844	1	74	theme	slices	126:131	arg1	preservation					133:144	fresh-cut apple slices preservation	110:144	fresh-cut apple slices preservation	110:144	carica leaves extract for fresh-cut apple slices preservation.					
36716844	10	75	theme	food	1448:1451	arg1	industry					1453:1460	the food industry	1444:1460	the food industry	1444:1460	According to our findings, Chitosan/KC/FLE film might be employed as a possible food packaging material in the food industry.					
35405211	0	0	theme	cell	92:95	arg1	types					97:101	different cell types	82:101	different cell types	82:101	Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles.					
35405211	2	1	theme	fluorescence	348:359	arg1	microspectrometry					310:326	Infra-red microspectrometry	300:326	Infra-red microspectrometry	300:326	Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale.					
35405211	2	1	theme	fluorescence	348:359	arg1	recovery					361:368	fluorescence recovery	348:368	fluorescence recovery after photobleaching and fluorescence imaging	348:414	Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale.					
35405211	0	2	theme	different	82:90	arg1	types					97:101	different cell types	82:101	different cell types	82:101	Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles.					
35405211	4	3	theme	recalcitrant	700:711	arg1	cells					713:717	the most recalcitrant cells	691:717	the most recalcitrant cells	691:717	Enzymes act sequentially from the cell walls rich in accessible cellulose to the most recalcitrant cells.					
35405211	3	4	theme	different	562:570	arg1	extent					572:577	different extent	562:577	different extent	562:577	Depending on their polymer composition and organisation, cell types exhibits different extent and rate of enzymatic degradation.					
35405211	5	5	theme	heterogeneous	757:769	arg1	distribution					771:782	the heterogeneous distribution	753:782	the heterogeneous distribution of enzymes in the liquid medium	753:814	This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.					
35405211	4	6	theme	most	695:698	arg1	cells					713:717	the most recalcitrant cells	691:717	the most recalcitrant cells	691:717	Enzymes act sequentially from the cell walls rich in accessible cellulose to the most recalcitrant cells.					
35405211	5	7	theme	cell	886:889	arg1	type					878:881	the type	874:881	the type of cell	874:889	This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.					
35405211	0	8	theme	different	108:116	arg1	profiles					118:125	different profiles	108:125	different profiles	108:125	Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles.					
35405211	5	9	attach	linked	743:748	arg1	mechanisms					846:855	the adsorption/desorption mechanisms	820:855	the adsorption/desorption mechanisms that differ with the type of cell	820:889	This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.					
35405211	5	9	attach	linked	743:748	arg1	distribution					771:782	the heterogeneous distribution	753:782	the heterogeneous distribution of enzymes in the liquid medium	753:814	This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.					
35405211	5	9	attach	linked	743:748	arg2	phenomenon					725:734	This phenomenon	720:734	This phenomenon	720:734	This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.					
35405211	1	10	theme	enzymatic	169:177	arg1	degradation					179:189	the enzymatic degradation	165:189	the enzymatic degradation of maize cell walls	165:209	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
35405211	5	11	theme	adsorption/desorption	824:844	arg1	mechanisms					846:855	the adsorption/desorption mechanisms	820:855	the adsorption/desorption mechanisms that differ with the type of cell	820:889	This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.					
35405211	5	12	from	mechanisms	846:855	arg1	medium					809:814	the liquid medium	798:814	the liquid medium	798:814	This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.					
35405211	2	13	theme	Infra-red	300:308	arg1	microspectrometry					310:326	Infra-red microspectrometry	300:326	Infra-red microspectrometry	300:326	Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale.					
35405211	2	13	theme	Infra-red	300:308	arg1	spectrometry					334:345	mass spectrometry	329:345	mass spectrometry	329:345	Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale.					
35405211	2	13	theme	Infra-red	300:308	arg1	recovery					361:368	fluorescence recovery	348:368	fluorescence recovery after photobleaching and fluorescence imaging	348:414	Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale.					
35405211	4	14	from	cellulose	678:686	arg1	rich					659:662	rich	659:662	rich	659:662	Enzymes act sequentially from the cell walls rich in accessible cellulose to the most recalcitrant cells.					
35405211	1	15	theme	degradation	179:189	arg1	view					157:160	a dynamic view	147:160	a dynamic view of the enzymatic degradation of maize cell walls	147:209	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
35405211	0	16	theme	Real-time	0:8	arg1	imaging					10:16	Real-time imaging	0:16	Real-time imaging of enzymatic degradation of pretreated maize internodes	0:72	Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles.					
35405211	1	17	theme	hot	256:258	arg1	internodes					288:297	hot water pretreated maize stem internodes	256:297	hot water pretreated maize stem internodes	256:297	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
35405211	3	18	theme	enzymatic	591:599	arg1	degradation					601:611	enzymatic degradation	591:611	enzymatic degradation	591:611	Depending on their polymer composition and organisation, cell types exhibits different extent and rate of enzymatic degradation.					
35405211	5	19	from	distribution	771:782	arg1	medium					809:814	the liquid medium	798:814	the liquid medium	798:814	This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.					
35405211	0	20	theme	enzymatic	21:29	arg1	degradation					31:41	enzymatic degradation	21:41	enzymatic degradation of pretreated maize internodes	21:72	Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles.					
35405211	1	21	theme	water	260:264	arg1	internodes					288:297	hot water pretreated maize stem internodes	256:297	hot water pretreated maize stem internodes	256:297	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
35405211	1	22	theme	maize	194:198	arg1	walls					205:209	maize cell walls	194:209	maize cell walls	194:209	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
35405211	2	23	theme	enzymatic	445:453	arg1	hydrolysis					455:464	enzymatic hydrolysis	445:464	enzymatic hydrolysis	445:464	Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale.					
35405211	1	24	theme	pretreated	266:275	arg1	internodes					288:297	hot water pretreated maize stem internodes	256:297	hot water pretreated maize stem internodes	256:297	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
35405211	4	25	from	rich	659:662	arg1	cellulose					678:686	accessible cellulose	667:686	accessible cellulose to the most recalcitrant cells	667:717	Enzymes act sequentially from the cell walls rich in accessible cellulose to the most recalcitrant cells.					
35405211	1	26	theme	cell	200:203	arg1	walls					205:209	maize cell walls	194:209	maize cell walls	194:209	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
35405211	5	27	theme	liquid	802:807	arg1	medium					809:814	the liquid medium	798:814	the liquid medium	798:814	This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.					
35405211	1	28	theme	maize	277:281	arg1	internodes					288:297	hot water pretreated maize stem internodes	256:297	hot water pretreated maize stem internodes	256:297	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
35405211	4	29	theme	accessible	667:676	arg1	cellulose					678:686	accessible cellulose	667:686	accessible cellulose to the most recalcitrant cells	667:717	Enzymes act sequentially from the cell walls rich in accessible cellulose to the most recalcitrant cells.					
35405211	1	30	theme	walls	205:209	arg1	degradation					179:189	the enzymatic degradation	165:189	the enzymatic degradation of maize cell walls	165:209	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
35405211	0	31	theme	degradation	31:41	arg1	imaging					10:16	Real-time imaging	0:16	Real-time imaging of enzymatic degradation of pretreated maize internodes	0:72	Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles.					
35405211	1	32	theme	stem	283:286	arg1	internodes					288:297	hot water pretreated maize stem internodes	256:297	hot water pretreated maize stem internodes	256:297	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
35405211	4	33	theme	cell	648:651	arg1	walls					653:657	the cell walls	644:657	the cell walls rich in accessible cellulose to the most recalcitrant cells	644:717	Enzymes act sequentially from the cell walls rich in accessible cellulose to the most recalcitrant cells.					
35405211	0	34	theme	maize	57:61	arg1	internodes					63:72	pretreated maize internodes	46:72	pretreated maize internodes	46:72	Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles.					
35405211	1	35	theme	internodes	288:297	arg1	recalcitrance					239:251	the recalcitrance	235:251	the recalcitrance of hot water pretreated maize stem internodes	235:297	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
35405211	5	36	theme	enzymes	787:793	arg1	mechanisms					846:855	the adsorption/desorption mechanisms	820:855	the adsorption/desorption mechanisms that differ with the type of cell	820:889	This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.					
35405211	5	36	theme	enzymes	787:793	arg1	distribution					771:782	the heterogeneous distribution	753:782	the heterogeneous distribution of enzymes in the liquid medium	753:814	This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.					
35405211	3	37	theme	degradation	601:611	arg1	rate					583:586	rate	583:586	rate of enzymatic degradation	583:611	Depending on their polymer composition and organisation, cell types exhibits different extent and rate of enzymatic degradation.					
35405211	3	37	theme	degradation	601:611	arg1	extent					572:577	different extent	562:577	different extent	562:577	Depending on their polymer composition and organisation, cell types exhibits different extent and rate of enzymatic degradation.					
35405211	3	38	theme	polymer	504:510	arg1	types					547:551	cell types	542:551	cell types	542:551	Depending on their polymer composition and organisation, cell types exhibits different extent and rate of enzymatic degradation.					
35405211	3	38	theme	polymer	504:510	arg1	composition					512:522	their polymer composition	498:522	their polymer composition	498:522	Depending on their polymer composition and organisation, cell types exhibits different extent and rate of enzymatic degradation.					
35405211	0	39	theme	pretreated	46:55	arg1	internodes					63:72	pretreated maize internodes	46:72	pretreated maize internodes	46:72	Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles.					
35405211	2	40	theme	cell	473:476	arg1	scale					478:482	the cell scale	469:482	the cell scale	469:482	Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale.					
35405211	2	41	theme	mass	329:332	arg1	microspectrometry					310:326	Infra-red microspectrometry	300:326	Infra-red microspectrometry	300:326	Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale.					
35405211	2	41	theme	mass	329:332	arg1	spectrometry					334:345	mass spectrometry	329:345	mass spectrometry	329:345	Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale.					
35405211	0	42	contain	have	103:106	arg2	profiles					118:125	different profiles	108:125	different profiles	108:125	Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles.					
35405211	0	42	contain	have	103:106	arg1	types					97:101	different cell types	82:101	different cell types	82:101	Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles.					
35405211	3	43	theme	cell	542:545	arg1	types					547:551	cell types	542:551	cell types	542:551	Depending on their polymer composition and organisation, cell types exhibits different extent and rate of enzymatic degradation.					
35405211	3	43	theme	cell	542:545	arg1	composition					512:522	their polymer composition	498:522	their polymer composition	498:522	Depending on their polymer composition and organisation, cell types exhibits different extent and rate of enzymatic degradation.					
35405211	4	44	theme	rich	659:662	arg1	walls					653:657	the cell walls	644:657	the cell walls rich in accessible cellulose to the most recalcitrant cells	644:717	Enzymes act sequentially from the cell walls rich in accessible cellulose to the most recalcitrant cells.					
35405211	2	45	theme	fluorescence	395:406	arg1	imaging					408:414	fluorescence imaging	395:414	fluorescence imaging	395:414	Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale.					
35405211	1	46	theme	new	222:224	arg1	light					226:230	new light	222:230	new light	222:230	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
35405211	0	47	theme	internodes	63:72	arg1	degradation					31:41	enzymatic degradation	21:41	enzymatic degradation of pretreated maize internodes	21:72	Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles.					
35405211	1	48	theme	dynamic	149:155	arg1	view					157:160	a dynamic view	147:160	a dynamic view of the enzymatic degradation of maize cell walls	147:209	This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes.					
36369784	9	0	theme	highest	1416:1422	arg1	content					1424:1430	the highest content	1412:1430	the highest content of quercetin	1412:1443	Cytological experiments showed that the biocompatibility of rat bone marrow mesenchymal stromal cells was promoted by quercetin-loaded membranes, exclusively in the group with the highest content of quercetin.					
36369784	9	1	theme	rat	1296:1298	arg1	cells					1332:1336	rat bone marrow mesenchymal stromal cells	1296:1336	rat bone marrow mesenchymal stromal cells	1296:1336	Cytological experiments showed that the biocompatibility of rat bone marrow mesenchymal stromal cells was promoted by quercetin-loaded membranes, exclusively in the group with the highest content of quercetin.					
36369784	8	2	theme	live/dead	1216:1224	arg1	staining					1226:1233	live/dead staining	1216:1233	live/dead staining	1216:1233	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, were verified by spread-plate, scanning electron microscopy, and live/dead staining.					
36369784	4	3	theme	responsible	563:573	arg1	Quercetin					532:540	Quercetin	532:540	Quercetin	532:540	Quercetin, an active flavonoid responsible for fulfilling various plant functions, was introduced as an antibacterial agent to be loaded into the membrane during preparation.					
36369784	4	3	theme	responsible	563:573	arg1	flavonoid					553:561	an active flavonoid	543:561	an active flavonoid responsible for fulfilling various plant functions	543:612	Quercetin, an active flavonoid responsible for fulfilling various plant functions, was introduced as an antibacterial agent to be loaded into the membrane during preparation.					
36369784	9	4	theme	bone	1300:1303	arg1	cells					1332:1336	rat bone marrow mesenchymal stromal cells	1296:1336	rat bone marrow mesenchymal stromal cells	1296:1336	Cytological experiments showed that the biocompatibility of rat bone marrow mesenchymal stromal cells was promoted by quercetin-loaded membranes, exclusively in the group with the highest content of quercetin.					
36369784	1	5	theme	bacterial	214:222	arg1	infections					224:233	bacterial infections	214:233	bacterial infections	214:233	Peri-implantitis is characterized by inflammation resulting from bacterial infections in peri-implant connective tissue.					
36369784	6	6	dep	Fourier	840:846	arg1	transform					848:856	transform	848:856	transform infrared spectroscopy	848:878	Fluorescent microscope, Attenuated Total Reflection Fourier transform infrared spectroscopy, and X-ray diffraction results verified the entrapment of quercetin.					
36369784	12	7	theme	potential	1689:1697	arg1	strategy					1699:1706	a potential strategy	1687:1706	a potential strategy to functionalize Ti implants	1687:1735	Thus, biocompatible and antibacterial membranes could be a potential strategy to functionalize Ti implants.					
36369784	12	7	theme	potential	1689:1697	arg1	membranes					1668:1676	biocompatible and antibacterial membranes	1636:1676	biocompatible and antibacterial membranes	1636:1676	Thus, biocompatible and antibacterial membranes could be a potential strategy to functionalize Ti implants.					
36369784	0	8	theme	electrophoretic	121:135	arg1	deposition					137:146	electrophoretic deposition	121:146	electrophoretic deposition	121:146	Investigation on characterization of novel anti-bacterial chitosan/gelatin composite membranes loaded with quercetin via electrophoretic deposition.					
36369784	0	9	from	Investigation	0:12	arg1	characterization					17:32	characterization	17:32	characterization of novel anti-bacterial chitosan/gelatin composite membranes	17:93	Investigation on characterization of novel anti-bacterial chitosan/gelatin composite membranes loaded with quercetin via electrophoretic deposition.					
36369784	9	10	theme	marrow	1305:1310	arg1	cells					1332:1336	rat bone marrow mesenchymal stromal cells	1296:1336	rat bone marrow mesenchymal stromal cells	1296:1336	Cytological experiments showed that the biocompatibility of rat bone marrow mesenchymal stromal cells was promoted by quercetin-loaded membranes, exclusively in the group with the highest content of quercetin.					
36369784	10	11	theme	antineoplastic	1492:1505	arg1	activity					1507:1514	the antineoplastic activity	1488:1514	the antineoplastic activity of MG-63 cells	1488:1529	The quercetin-loaded groups also enhanced the antineoplastic activity of MG-63 cells.					
36369784	10	12	theme	MG-63	1519:1523	arg1	cells					1525:1529	MG-63 cells	1519:1529	MG-63 cells	1519:1529	The quercetin-loaded groups also enhanced the antineoplastic activity of MG-63 cells.					
36369784	9	13	theme	quercetin	1435:1443	arg1	content					1424:1430	the highest content	1412:1430	the highest content of quercetin	1412:1443	Cytological experiments showed that the biocompatibility of rat bone marrow mesenchymal stromal cells was promoted by quercetin-loaded membranes, exclusively in the group with the highest content of quercetin.					
36369784	6	14	theme	Reflection	829:838	arg1	Fourier					840:846	Attenuated Total Reflection Fourier	812:846	Attenuated Total Reflection Fourier transform infrared spectroscopy	812:878	Fluorescent microscope, Attenuated Total Reflection Fourier transform infrared spectroscopy, and X-ray diffraction results verified the entrapment of quercetin.					
36369784	6	15	theme	X-ray	885:889	arg1	diffraction					891:901	X-ray diffraction	885:901	X-ray diffraction	885:901	Fluorescent microscope, Attenuated Total Reflection Fourier transform infrared spectroscopy, and X-ray diffraction results verified the entrapment of quercetin.					
36369784	9	16	with	group	1401:1405	arg1	content					1424:1430	the highest content	1412:1430	the highest content of quercetin	1412:1443	Cytological experiments showed that the biocompatibility of rat bone marrow mesenchymal stromal cells was promoted by quercetin-loaded membranes, exclusively in the group with the highest content of quercetin.					
36369784	4	17	theme	antibacterial	636:648	arg1	Quercetin					532:540	Quercetin	532:540	Quercetin	532:540	Quercetin, an active flavonoid responsible for fulfilling various plant functions, was introduced as an antibacterial agent to be loaded into the membrane during preparation.					
36369784	4	17	theme	antibacterial	636:648	arg1	agent					650:654	an antibacterial agent	633:654	an antibacterial agent to be loaded into the membrane during preparation	633:704	Quercetin, an active flavonoid responsible for fulfilling various plant functions, was introduced as an antibacterial agent to be loaded into the membrane during preparation.					
36369784	9	18	theme	stromal	1324:1330	arg1	cells					1332:1336	rat bone marrow mesenchymal stromal cells	1296:1336	rat bone marrow mesenchymal stromal cells	1296:1336	Cytological experiments showed that the biocompatibility of rat bone marrow mesenchymal stromal cells was promoted by quercetin-loaded membranes, exclusively in the group with the highest content of quercetin.					
36369784	6	19	dep	transform	848:856	arg1	infrared					858:865	infrared	858:865	transform infrared spectroscopy	848:878	Fluorescent microscope, Attenuated Total Reflection Fourier transform infrared spectroscopy, and X-ray diffraction results verified the entrapment of quercetin.					
36369784	8	20	from	effects	1024:1030	arg1	aureus					1095:1100	Staphylococcus aureus	1080:1100	Staphylococcus aureus	1080:1100	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, were verified by spread-plate, scanning electron microscopy, and live/dead staining.					
36369784	8	20	from	effects	1024:1030	arg1	bacteria					1062:1069	Gram-positive bacteria	1048:1069	Gram-positive bacteria	1048:1069	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, were verified by spread-plate, scanning electron microscopy, and live/dead staining.					
36369784	8	20	from	effects	1024:1030	arg1	aureus					1143:1148	Staphylococcus aureus	1128:1148	methicillin-resistant Staphylococcus aureus	1106:1148	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, were verified by spread-plate, scanning electron microscopy, and live/dead staining.					
36369784	6	21	theme	quercetin	938:946	arg1	entrapment					924:933	the entrapment	920:933	the entrapment of quercetin	920:946	Fluorescent microscope, Attenuated Total Reflection Fourier transform infrared spectroscopy, and X-ray diffraction results verified the entrapment of quercetin.					
36369784	2	22	theme	implants	439:446	arg1	surface					414:420	the surface	410:420	the surface of titanium (Ti) implants	410:446	The purpose of this study was to prepare and characterize chitosan/gelatin (CSG)-based membranes with antibacterial agents to functionalize the surface of titanium (Ti) implants.					
36369784	2	23	theme	antibacterial	372:384	arg1	agents					386:391	antibacterial agents	372:391	antibacterial agents	372:391	The purpose of this study was to prepare and characterize chitosan/gelatin (CSG)-based membranes with antibacterial agents to functionalize the surface of titanium (Ti) implants.					
36369784	1	24	theme	peri-implant	238:249	arg1	tissue					262:267	peri-implant connective tissue	238:267	peri-implant connective tissue	238:267	Peri-implantitis is characterized by inflammation resulting from bacterial infections in peri-implant connective tissue.					
36369784	10	25	theme	quercetin-loaded	1450:1465	arg1	groups					1467:1472	The quercetin-loaded groups	1446:1472	The quercetin-loaded groups	1446:1472	The quercetin-loaded groups also enhanced the antineoplastic activity of MG-63 cells.					
36369784	3	26	theme	Ti	480:481	arg1	substrates					483:492	Ti substrates	480:492	Ti substrates	480:492	CSG membranes were prepared on Ti substrates via electrophoretic deposition (EPD).					
36369784	9	27	theme	mesenchymal	1312:1322	arg1	cells					1332:1336	rat bone marrow mesenchymal stromal cells	1296:1336	rat bone marrow mesenchymal stromal cells	1296:1336	Cytological experiments showed that the biocompatibility of rat bone marrow mesenchymal stromal cells was promoted by quercetin-loaded membranes, exclusively in the group with the highest content of quercetin.					
36369784	8	28	theme	electron	1191:1198	arg1	microscopy					1200:1209	scanning electron microscopy	1182:1209	scanning electron microscopy	1182:1209	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, were verified by spread-plate, scanning electron microscopy, and live/dead staining.					
36369784	10	29	theme	cells	1525:1529	arg1	activity					1507:1514	the antineoplastic activity	1488:1514	the antineoplastic activity of MG-63 cells	1488:1529	The quercetin-loaded groups also enhanced the antineoplastic activity of MG-63 cells.					
36369784	8	30	theme	scanning	1182:1189	arg1	microscopy					1200:1209	scanning electron microscopy	1182:1209	scanning electron microscopy	1182:1209	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, were verified by spread-plate, scanning electron microscopy, and live/dead staining.					
36369784	9	31	theme	quercetin-loaded	1354:1369	arg1	membranes					1371:1379	quercetin-loaded membranes	1354:1379	quercetin-loaded membranes	1354:1379	Cytological experiments showed that the biocompatibility of rat bone marrow mesenchymal stromal cells was promoted by quercetin-loaded membranes, exclusively in the group with the highest content of quercetin.					
36369784	2	32	theme	-based	350:355	arg1	membranes					357:365	chitosan/gelatin (CSG)-based membranes	328:365	chitosan/gelatin (CSG)-based membranes	328:365	The purpose of this study was to prepare and characterize chitosan/gelatin (CSG)-based membranes with antibacterial agents to functionalize the surface of titanium (Ti) implants.					
36369784	0	33	theme	anti-bacterial	43:56	arg1	membranes					85:93	novel anti-bacterial chitosan/gelatin composite membranes	37:93	novel anti-bacterial chitosan/gelatin composite membranes	37:93	Investigation on characterization of novel anti-bacterial chitosan/gelatin composite membranes loaded with quercetin via electrophoretic deposition.					
36369784	2	34	theme	Ti	435:436	arg1	implants					439:446	titanium (Ti) implants	425:446	titanium (Ti) implants	425:446	The purpose of this study was to prepare and characterize chitosan/gelatin (CSG)-based membranes with antibacterial agents to functionalize the surface of titanium (Ti) implants.					
36369784	3	35	theme	electrophoretic	498:512	arg1	deposition					514:523	electrophoretic deposition	498:523	electrophoretic deposition (EPD)	498:529	CSG membranes were prepared on Ti substrates via electrophoretic deposition (EPD).					
36369784	3	35	theme	electrophoretic	498:512	arg1	EPD					526:528	EPD	526:528	EPD	526:528	CSG membranes were prepared on Ti substrates via electrophoretic deposition (EPD).					
36369784	0	36	theme	novel	37:41	arg1	membranes					85:93	novel anti-bacterial chitosan/gelatin composite membranes	37:93	novel anti-bacterial chitosan/gelatin composite membranes	37:93	Investigation on characterization of novel anti-bacterial chitosan/gelatin composite membranes loaded with quercetin via electrophoretic deposition.					
36369784	7	37	theme	mass	982:985	arg1	mass					982:985	mass	982:985	mass	982:985	The membranes swelled by 150% of mass after rehydration.					
36369784	7	37	theme	mass	982:985	arg1	%					977:977	150%	974:977	150% of mass	974:985	The membranes swelled by 150% of mass after rehydration.					
36369784	2	38	theme	study	290:294	arg1	purpose					274:280	The purpose	270:280	The purpose of this study	270:294	The purpose of this study was to prepare and characterize chitosan/gelatin (CSG)-based membranes with antibacterial agents to functionalize the surface of titanium (Ti) implants.					
36369784	9	39	theme	Cytological	1236:1246	arg1	experiments					1248:1258	Cytological experiments	1236:1258	Cytological experiments	1236:1258	Cytological experiments showed that the biocompatibility of rat bone marrow mesenchymal stromal cells was promoted by quercetin-loaded membranes, exclusively in the group with the highest content of quercetin.					
36369784	3	40	theme	CSG	449:451	arg1	membranes					453:461	CSG membranes	449:461	CSG membranes	449:461	CSG membranes were prepared on Ti substrates via electrophoretic deposition (EPD).					
36369784	11	41	theme	quercetin-loaded	1561:1576	arg1	membranes					1582:1590	quercetin-loaded CSG membranes	1561:1590	quercetin-loaded CSG membranes	1561:1590	These results suggested that quercetin-loaded CSG membranes were successfully fabricated via EPD.					
36369784	5	42	theme	quercetin-loaded	726:741	arg1	membranes					747:755	quercetin-loaded CSG membranes	726:755	quercetin-loaded CSG membranes	726:755	The fabrication of quercetin-loaded CSG membranes via EPD was also investigated.					
36369784	0	43	theme	membranes	85:93	arg1	characterization					17:32	characterization	17:32	characterization of novel anti-bacterial chitosan/gelatin composite membranes	17:93	Investigation on characterization of novel anti-bacterial chitosan/gelatin composite membranes loaded with quercetin via electrophoretic deposition.					
36369784	1	44	theme	connective	251:260	arg1	tissue					262:267	peri-implant connective tissue	238:267	peri-implant connective tissue	238:267	Peri-implantitis is characterized by inflammation resulting from bacterial infections in peri-implant connective tissue.					
36369784	0	45	theme	chitosan/gelatin	58:73	arg1	membranes					85:93	novel anti-bacterial chitosan/gelatin composite membranes	37:93	novel anti-bacterial chitosan/gelatin composite membranes	37:93	Investigation on characterization of novel anti-bacterial chitosan/gelatin composite membranes loaded with quercetin via electrophoretic deposition.					
36369784	4	46	theme	plant	598:602	arg1	functions					604:612	various plant functions	590:612	various plant functions	590:612	Quercetin, an active flavonoid responsible for fulfilling various plant functions, was introduced as an antibacterial agent to be loaded into the membrane during preparation.					
36369784	5	47	theme	CSG	743:745	arg1	membranes					747:755	quercetin-loaded CSG membranes	726:755	quercetin-loaded CSG membranes	726:755	The fabrication of quercetin-loaded CSG membranes via EPD was also investigated.					
36369784	12	48	theme	biocompatible	1636:1648	arg1	strategy					1699:1706	a potential strategy	1687:1706	a potential strategy to functionalize Ti implants	1687:1735	Thus, biocompatible and antibacterial membranes could be a potential strategy to functionalize Ti implants.					
36369784	12	48	theme	biocompatible	1636:1648	arg1	membranes					1668:1676	biocompatible and antibacterial membranes	1636:1676	biocompatible and antibacterial membranes	1636:1676	Thus, biocompatible and antibacterial membranes could be a potential strategy to functionalize Ti implants.					
36369784	6	49	theme	Total	823:827	arg1	Fourier					840:846	Attenuated Total Reflection Fourier	812:846	Attenuated Total Reflection Fourier transform infrared spectroscopy	812:878	Fluorescent microscope, Attenuated Total Reflection Fourier transform infrared spectroscopy, and X-ray diffraction results verified the entrapment of quercetin.					
36369784	4	50	theme	various	590:596	arg1	functions					604:612	various plant functions	590:612	various plant functions	590:612	Quercetin, an active flavonoid responsible for fulfilling various plant functions, was introduced as an antibacterial agent to be loaded into the membrane during preparation.					
36369784	5	51	theme	membranes	747:755	arg1	fabrication					711:721	The fabrication	707:721	The fabrication of quercetin-loaded CSG membranes via EPD	707:763	The fabrication of quercetin-loaded CSG membranes via EPD was also investigated.					
36369784	9	52	theme	cells	1332:1336	arg1	biocompatibility					1276:1291	the biocompatibility	1272:1291	the biocompatibility of rat bone marrow mesenchymal stromal cells	1272:1336	Cytological experiments showed that the biocompatibility of rat bone marrow mesenchymal stromal cells was promoted by quercetin-loaded membranes, exclusively in the group with the highest content of quercetin.					
36369784	6	53	theme	Attenuated	812:821	arg1	Fourier					840:846	Attenuated Total Reflection Fourier	812:846	Attenuated Total Reflection Fourier transform infrared spectroscopy	812:878	Fluorescent microscope, Attenuated Total Reflection Fourier transform infrared spectroscopy, and X-ray diffraction results verified the entrapment of quercetin.					
36369784	8	54	theme	Gram-positive	1048:1060	arg1	aureus					1095:1100	Staphylococcus aureus	1080:1100	Staphylococcus aureus	1080:1100	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, were verified by spread-plate, scanning electron microscopy, and live/dead staining.					
36369784	8	54	theme	Gram-positive	1048:1060	arg1	bacteria					1062:1069	Gram-positive bacteria	1048:1069	Gram-positive bacteria	1048:1069	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, were verified by spread-plate, scanning electron microscopy, and live/dead staining.					
36369784	8	54	theme	Gram-positive	1048:1060	arg1	aureus					1143:1148	Staphylococcus aureus	1128:1148	methicillin-resistant Staphylococcus aureus	1106:1148	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, were verified by spread-plate, scanning electron microscopy, and live/dead staining.					
36369784	8	55	theme	antibacterial	1010:1022	arg1	effects					1024:1030	The antibacterial effects	1006:1030	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus,	1006:1149	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, were verified by spread-plate, scanning electron microscopy, and live/dead staining.					
36369784	12	56	theme	antibacterial	1654:1666	arg1	strategy					1699:1706	a potential strategy	1687:1706	a potential strategy to functionalize Ti implants	1687:1735	Thus, biocompatible and antibacterial membranes could be a potential strategy to functionalize Ti implants.					
36369784	12	56	theme	antibacterial	1654:1666	arg1	membranes					1668:1676	biocompatible and antibacterial membranes	1636:1676	biocompatible and antibacterial membranes	1636:1676	Thus, biocompatible and antibacterial membranes could be a potential strategy to functionalize Ti implants.					
36369784	8	57	theme	quercetin	1035:1043	arg1	effects					1024:1030	The antibacterial effects	1006:1030	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus,	1006:1149	The antibacterial effects of quercetin on Gram-positive bacteria, such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, were verified by spread-plate, scanning electron microscopy, and live/dead staining.					
36369784	12	58	theme	Ti	1725:1726	arg1	implants					1728:1735	Ti implants	1725:1735	Ti implants	1725:1735	Thus, biocompatible and antibacterial membranes could be a potential strategy to functionalize Ti implants.					
36369784	6	59	dep	microscope	800:809	arg1	results					903:909	results	903:909	results	903:909	Fluorescent microscope, Attenuated Total Reflection Fourier transform infrared spectroscopy, and X-ray diffraction results verified the entrapment of quercetin.					
36369784	0	60	theme	composite	75:83	arg1	membranes					85:93	novel anti-bacterial chitosan/gelatin composite membranes	37:93	novel anti-bacterial chitosan/gelatin composite membranes	37:93	Investigation on characterization of novel anti-bacterial chitosan/gelatin composite membranes loaded with quercetin via electrophoretic deposition.					
36369784	4	61	theme	active	546:551	arg1	Quercetin					532:540	Quercetin	532:540	Quercetin	532:540	Quercetin, an active flavonoid responsible for fulfilling various plant functions, was introduced as an antibacterial agent to be loaded into the membrane during preparation.					
36369784	4	61	theme	active	546:551	arg1	flavonoid					553:561	an active flavonoid	543:561	an active flavonoid responsible for fulfilling various plant functions	543:612	Quercetin, an active flavonoid responsible for fulfilling various plant functions, was introduced as an antibacterial agent to be loaded into the membrane during preparation.					
36369784	6	62	theme	Fluorescent	788:798	arg1	microscope					800:809	Fluorescent microscope	788:809	Fluorescent microscope	788:809	Fluorescent microscope, Attenuated Total Reflection Fourier transform infrared spectroscopy, and X-ray diffraction results verified the entrapment of quercetin.					
36369784	2	63	theme	titanium	425:432	arg1	implants					439:446	titanium (Ti) implants	425:446	titanium (Ti) implants	425:446	The purpose of this study was to prepare and characterize chitosan/gelatin (CSG)-based membranes with antibacterial agents to functionalize the surface of titanium (Ti) implants.					
36369784	11	64	theme	CSG	1578:1580	arg1	membranes					1582:1590	quercetin-loaded CSG membranes	1561:1590	quercetin-loaded CSG membranes	1561:1590	These results suggested that quercetin-loaded CSG membranes were successfully fabricated via EPD.					
36258114	1	0	theme	new	297:299	arg1	Fe3O4					328:332	new designed [GTA-(PDA-g-DAC) @Fe3O4]	297:333	new designed [GTA-(PDA-g-DAC) @Fe3O4]	297:333	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	9	1	theme	Fenton	1820:1825	arg1	technology					1860:1869	a promising treatment technology	1838:1869	a promising treatment technology for OMW effluents	1838:1887	The gathered findings gave the evidence that sono-heterogeneous catalytic Fenton process is a promising treatment technology for OMW effluents.					
36258114	9	1	theme	Fenton	1820:1825	arg1	process					1827:1833	sono-heterogeneous catalytic Fenton process	1791:1833	sono-heterogeneous catalytic Fenton process	1791:1833	The gathered findings gave the evidence that sono-heterogeneous catalytic Fenton process is a promising treatment technology for OMW effluents.					
36258114	2	2	theme	significant	576:586	arg1	efficiency					600:609	significant degradation efficiency	576:609	significant degradation efficiency (75%)	576:615	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	2	2	theme	significant	576:586	arg1	%					614:614	75%	612:614	75%	612:614	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	2	3	with	g/L	710:712	arg1	/H2O2					723:727	US /H2O2	720:727	US /H2O2	720:727	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	1	4	theme	[GTA-	310:314	arg1	Fe3O4					328:332	new designed [GTA-(PDA-g-DAC) @Fe3O4]	297:333	new designed [GTA-(PDA-g-DAC) @Fe3O4]	297:333	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	3	5	dep	H2O2/US	819:825	arg1	%					815:815	13%	813:815	13%	813:815	The values obtained by US only were (13%), H2O2/US (18%), US/Fe3O4 (28%), and US /Fe3O4/H2O2(35%).					
36258114	3	6	theme	US	854:855	arg1	/Fe3O4/H2O2					857:867	US /Fe3O4/H2O2	854:867	US /Fe3O4/H2O2(35%)	854:872	The values obtained by US only were (13%), H2O2/US (18%), US/Fe3O4 (28%), and US /Fe3O4/H2O2(35%).					
36258114	3	6	theme	US	854:855	arg1	%					871:871	35%	869:871	35%	869:871	The values obtained by US only were (13%), H2O2/US (18%), US/Fe3O4 (28%), and US /Fe3O4/H2O2(35%).					
36258114	1	7	theme	wastewater	204:213	arg1	characteristics					231:245	the olive mill wastewater (OMW) treatment characteristics	189:245	the olive mill wastewater (OMW) treatment characteristics	189:245	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	2	8	theme	=	706:706	arg1	g/L					710:712	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L)	636:713	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2	636:727	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	1	9	theme	properties	472:481	arg1	measurements					483:494	magnetic properties measurements	463:494	magnetic properties measurements	463:494	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	1	10	theme	OMW	216:218	arg1	characteristics					231:245	the olive mill wastewater (OMW) treatment characteristics	189:245	the olive mill wastewater (OMW) treatment characteristics	189:245	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	7	11	theme	oxygen	1518:1523	arg1	rate					1548:1551	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	6	12	theme	parameters	1168:1177	arg1	effect					1136:1141	the concomitant effect	1120:1141	the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4]	1120:1215	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	1	13	theme	treatment	221:229	arg1	characteristics					231:245	the olive mill wastewater (OMW) treatment characteristics	189:245	the olive mill wastewater (OMW) treatment characteristics	189:245	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	6	14	dep	optimized	1158:1166	arg1	degradation					1146:1156	degradation	1146:1156	degradation	1146:1156	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	7	15	theme	efficient	1413:1421	arg1	rates					1435:1439	efficient degradation rates	1413:1439	efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1413:1580	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	1	16	dep	process	283:289	arg1	using					291:295	using	291:295	using new designed [GTA-(PDA-g-DAC) @Fe3O4]	291:333	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	1	16	dep	process	283:289	arg1	characterized					339:351	characterized	339:351	characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis	339:538	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	4	17	theme	@	930:930	arg1	Fe3O4					931:935	@Fe3O4	930:935	@Fe3O4	930:935	The catalytic findings have shown that [GTA-(PDA-g-DAC)@Fe3O4] exhibited good properties for OMW compound's degradation.					
36258114	8	18	theme	magnetic	1677:1684	arg1	catalyst					1686:1693	The prepared magnetic catalyst	1664:1693	The prepared magnetic catalyst	1664:1693	The prepared magnetic catalyst exhibited a good stability during repeated cycles.					
36258114	6	19	theme	significant	1357:1367	arg1	decolorization					1369:1382	a significant decolorization	1355:1382	a significant decolorization	1355:1382	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	2	20	theme	@	686:686	arg1	g/L					710:712	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L)	636:713	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2	636:727	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	2	21	theme	preliminary	543:553	arg1	study					563:567	A preliminary removal study	541:567	A preliminary removal study	541:567	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	2	22	theme	PDA-g-DAC	676:684	arg1	g/L					710:712	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L)	636:713	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2	636:727	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	8	23	theme	good	1707:1710	arg1	stability					1712:1720	a good stability	1705:1720	a good stability	1705:1720	The prepared magnetic catalyst exhibited a good stability during repeated cycles.					
36258114	6	24	theme	solution	1334:1341	arg1	mineralization					1304:1317	an almost complete mineralization	1285:1317	an almost complete mineralization of aqueous OMW solution	1285:1341	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	1	25	theme	present	164:170	arg1	study					172:176	The present study	160:176	The present study	160:176	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	2	26	theme	[catalyst	695:703	arg1	g/L					710:712	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L)	636:713	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2	636:727	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	6	27	theme	PDA-g-DAC	1198:1206	arg1	Fe3O4					1210:1214	[GTA-(PDA-g-DAC) @Fe3O4	1192:1214	[GTA-(PDA-g-DAC) @Fe3O4	1192:1214	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	6	28	theme	aqueous	1322:1328	arg1	solution					1334:1341	aqueous OMW solution	1322:1341	aqueous OMW solution	1322:1341	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	5	29	theme	degradation	1074:1084	arg1	levels					1098:1103	the degradation substantial levels	1070:1103	the degradation substantial levels	1070:1103	The sonocatalytic process coupling and extra oxidant addition resulted in the degradation substantial levels.					
36258114	0	30	theme	matter	88:93	arg1	degradation					39:49	An optimized sono-heterogeneous Fenton degradation	0:49	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked	0:137	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked developed cellulose.					
36258114	6	31	dep	H2O2	1180:1183	arg1	Fe3O4					1210:1214	[GTA-(PDA-g-DAC) @Fe3O4	1192:1214	[GTA-(PDA-g-DAC) @Fe3O4	1192:1214	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	1	32	theme	olive	193:197	arg1	characteristics					231:245	the olive mill wastewater (OMW) treatment characteristics	189:245	the olive mill wastewater (OMW) treatment characteristics	189:245	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	7	33	theme	total	1476:1480	arg1	compounds					1488:1496	total amino compounds	1476:1496	total amino compounds (TAC)	1476:1502	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	7	33	theme	total	1476:1480	arg1	TAC					1499:1501	TAC	1499:1501	TAC	1499:1501	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	0	34	theme	new	98:100	arg1	glutarlaldehyde-crosslinked					111:137	new magnetic glutarlaldehyde-crosslinked	98:137	new magnetic glutarlaldehyde-crosslinked	98:137	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked developed cellulose.					
36258114	8	35	theme	repeated	1729:1736	arg1	cycles					1738:1743	repeated cycles	1729:1743	repeated cycles	1729:1743	The prepared magnetic catalyst exhibited a good stability during repeated cycles.					
36258114	9	36	theme	sono-heterogeneous	1791:1808	arg1	technology					1860:1869	a promising treatment technology	1838:1869	a promising treatment technology for OMW effluents	1838:1887	The gathered findings gave the evidence that sono-heterogeneous catalytic Fenton process is a promising treatment technology for OMW effluents.					
36258114	9	36	theme	sono-heterogeneous	1791:1808	arg1	process					1827:1833	sono-heterogeneous catalytic Fenton process	1791:1833	sono-heterogeneous catalytic Fenton process	1791:1833	The gathered findings gave the evidence that sono-heterogeneous catalytic Fenton process is a promising treatment technology for OMW effluents.					
36258114	5	37	theme	extra	1035:1039	arg1	addition					1049:1056	extra oxidant addition	1035:1056	extra oxidant addition	1035:1056	The sonocatalytic process coupling and extra oxidant addition resulted in the degradation substantial levels.					
36258114	1	38	theme	@	327:327	arg1	Fe3O4					328:332	new designed [GTA-(PDA-g-DAC) @Fe3O4]	297:333	new designed [GTA-(PDA-g-DAC) @Fe3O4]	297:333	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	2	39	theme	[GTA-	670:674	arg1	g/L					710:712	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L)	636:713	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2	636:727	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	7	40	from	rates	1435:1439	arg1	compounds					1488:1496	total amino compounds	1476:1496	total amino compounds (TAC)	1476:1502	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	7	40	from	rates	1435:1439	arg1	TPC					1470:1472	TPC	1470:1472	TPC	1470:1472	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	7	40	from	rates	1435:1439	arg1	compounds					1459:1467	total phenolic compounds	1444:1467	total phenolic compounds (TPC)	1444:1473	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	7	40	from	rates	1435:1439	arg1	TAC					1499:1501	TAC	1499:1501	TAC	1499:1501	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	7	40	from	rates	1435:1439	arg1	rate					1548:1551	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	7	41	theme	phenolic	1450:1457	arg1	TPC					1470:1472	TPC	1470:1472	TPC	1470:1472	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	7	41	theme	phenolic	1450:1457	arg1	compounds					1459:1467	total phenolic compounds	1444:1467	total phenolic compounds (TPC)	1444:1473	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	1	42	dep	infrared	374:381	arg1	FTIR					384:387	FTIR	384:387	FTIR	384:387	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	2	43	theme	synthesized	649:659	arg1	g/L					710:712	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L)	636:713	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2	636:727	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	0	44	theme	sono-heterogeneous	13:30	arg1	degradation					39:49	An optimized sono-heterogeneous Fenton degradation	0:49	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked	0:137	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked developed cellulose.					
36258114	9	45	theme	promising	1840:1848	arg1	technology					1860:1869	a promising treatment technology	1838:1869	a promising treatment technology for OMW effluents	1838:1887	The gathered findings gave the evidence that sono-heterogeneous catalytic Fenton process is a promising treatment technology for OMW effluents.					
36258114	9	45	theme	promising	1840:1848	arg1	process					1827:1833	sono-heterogeneous catalytic Fenton process	1791:1833	sono-heterogeneous catalytic Fenton process	1791:1833	The gathered findings gave the evidence that sono-heterogeneous catalytic Fenton process is a promising treatment technology for OMW effluents.					
36258114	7	46	theme	Fenton	1648:1653	arg1	process					1655:1661	the optimized sono-heterogeneous catalytic Fenton process	1605:1661	the optimized sono-heterogeneous catalytic Fenton process	1605:1661	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	0	47	theme	wastewater	69:78	arg1	matter					88:93	olive-oil mill wastewater organic matter	54:93	olive-oil mill wastewater organic matter	54:93	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked developed cellulose.					
36258114	0	48	theme	olive-oil	54:62	arg1	matter					88:93	olive-oil mill wastewater organic matter	54:93	olive-oil mill wastewater organic matter	54:93	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked developed cellulose.					
36258114	7	49	theme	sono-heterogeneous	1619:1636	arg1	process					1655:1661	the optimized sono-heterogeneous catalytic Fenton process	1605:1661	the optimized sono-heterogeneous catalytic Fenton process	1605:1661	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	9	50	theme	OMW	1875:1877	arg1	effluents					1879:1887	OMW effluents	1875:1887	OMW effluents	1875:1887	The gathered findings gave the evidence that sono-heterogeneous catalytic Fenton process is a promising treatment technology for OMW effluents.					
36258114	5	51	theme	oxidant	1041:1047	arg1	addition					1049:1056	extra oxidant addition	1035:1056	extra oxidant addition	1035:1056	The sonocatalytic process coupling and extra oxidant addition resulted in the degradation substantial levels.					
36258114	2	52	theme	500WL-1	745:751	arg1	power					764:768	500WL-1 ultrasonic power	745:768	500WL-1 ultrasonic power (US)	745:773	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	1	53	theme	charge	515:520	arg1	analysis					531:538	zero charge (pH pzc) analysis	510:538	zero charge (pH pzc) analysis	510:538	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	1	53	theme	charge	515:520	arg1	pH					523:524	pH pzc	523:528	pH pzc	523:528	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	6	54	theme	T	1254:1254	arg1	°C					1259:1260	T 35 °C	1254:1260	T 35 °C for 70 min	1254:1271	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	1	55	theme	Fenton	276:281	arg1	process					283:289	a sono-heterogeneous Fenton process	255:289	a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis	255:538	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	7	56	theme	were	1553:1556	arg1	rate					1548:1551	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	4	57	theme	catalytic	879:887	arg1	findings					889:896	The catalytic findings	875:896	The catalytic findings	875:896	The catalytic findings have shown that [GTA-(PDA-g-DAC)@Fe3O4] exhibited good properties for OMW compound's degradation.					
36258114	9	58	theme	gathered	1750:1757	arg1	findings					1759:1766	The gathered findings	1746:1766	The gathered findings	1746:1766	The gathered findings gave the evidence that sono-heterogeneous catalytic Fenton process is a promising treatment technology for OMW effluents.					
36258114	2	59	theme	degradation	588:598	arg1	efficiency					600:609	significant degradation efficiency	576:609	significant degradation efficiency (75%)	576:615	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	2	59	theme	degradation	588:598	arg1	%					614:614	75%	612:614	75%	612:614	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	7	60	theme	oxidation	1538:1546	arg1	rate					1548:1551	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	1	61	theme	designed	301:308	arg1	Fe3O4					328:332	new designed [GTA-(PDA-g-DAC) @Fe3O4]	297:333	new designed [GTA-(PDA-g-DAC) @Fe3O4]	297:333	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	7	62	theme	COD	1533:1535	arg1	rate					1548:1551	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	4	63	theme	good	948:951	arg1	properties					953:962	good properties	948:962	good properties for OMW compound's degradation	948:993	The catalytic findings have shown that [GTA-(PDA-g-DAC)@Fe3O4] exhibited good properties for OMW compound's degradation.					
36258114	6	64	theme	complete	1295:1302	arg1	mineralization					1304:1317	an almost complete mineralization	1285:1317	an almost complete mineralization of aqueous OMW solution	1285:1341	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	1	65	theme	mill	199:202	arg1	characteristics					231:245	the olive mill wastewater (OMW) treatment characteristics	189:245	the olive mill wastewater (OMW) treatment characteristics	189:245	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	6	66	theme	@	1209:1209	arg1	Fe3O4					1210:1214	[GTA-(PDA-g-DAC) @Fe3O4	1192:1214	[GTA-(PDA-g-DAC) @Fe3O4	1192:1214	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	7	67	theme	demand	1525:1530	arg1	rate					1548:1551	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	6	68	theme	[GTA-	1192:1196	arg1	Fe3O4					1210:1214	[GTA-(PDA-g-DAC) @Fe3O4	1192:1214	[GTA-(PDA-g-DAC) @Fe3O4	1192:1214	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	1	69	theme	magnetic	463:470	arg1	measurements					483:494	magnetic properties measurements	463:494	magnetic properties measurements	463:494	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	7	70	theme	degradation	1423:1433	arg1	rates					1435:1439	efficient degradation rates	1413:1439	efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1413:1580	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	2	71	dep	power	764:768	arg1	US					771:772	US	771:772	US	771:772	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	6	72	dep	resulted	1273:1280	arg1	followed					1343:1350	followed	1343:1350	followed by a significant decolorization	1343:1382	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	7	73	theme	chemical	1509:1516	arg1	rate					1548:1551	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66	1509:1580	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	8	74	theme	prepared	1668:1675	arg1	catalyst					1686:1693	The prepared magnetic catalyst	1664:1693	The prepared magnetic catalyst	1664:1693	The prepared magnetic catalyst exhibited a good stability during repeated cycles.					
36258114	2	75	theme	US	720:721	arg1	/H2O2					723:727	US /H2O2	720:727	US /H2O2	720:727	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	6	76	theme	optimized	1158:1166	arg1	parameters					1168:1177	degradation optimized parameters	1146:1177	degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4]	1146:1215	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	1	77	theme	X-ray	404:408	arg1	diffraction					410:420	X-ray diffraction	404:420	X-ray diffraction (XRD)	404:426	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	1	77	theme	X-ray	404:408	arg1	XRD					423:425	XRD	423:425	XRD	423:425	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	2	78	dep	showed	569:574	arg1	occurred					617:624	occurred	617:624	showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US)	569:773	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	7	79	theme	Oxidation	1385:1393	arg1	results					1395:1401	Oxidation results	1385:1401	Oxidation results	1385:1401	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	2	80	theme	Fe3O4	687:691	arg1	g/L					710:712	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L)	636:713	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2	636:727	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	6	81	dep	parameters	1168:1177	arg1	H2O2					1180:1183	H2O2	1180:1183	H2O2 10 mM	1180:1189	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	2	82	theme	removal	555:561	arg1	study					563:567	A preliminary removal study	541:567	A preliminary removal study	541:567	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	6	83	theme	concomitant	1124:1134	arg1	effect					1136:1141	the concomitant effect	1120:1141	the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4]	1120:1215	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	4	84	dep	PDA-g-DAC	920:928	arg1	Fe3O4					931:935	@Fe3O4	930:935	@Fe3O4	930:935	The catalytic findings have shown that [GTA-(PDA-g-DAC)@Fe3O4] exhibited good properties for OMW compound's degradation.					
36258114	5	85	dep	process	1014:1020	arg1	addition					1049:1056	extra oxidant addition	1035:1056	extra oxidant addition	1035:1056	The sonocatalytic process coupling and extra oxidant addition resulted in the degradation substantial levels.					
36258114	5	85	dep	process	1014:1020	arg1	coupling					1022:1029	coupling	1022:1029	coupling	1022:1029	The sonocatalytic process coupling and extra oxidant addition resulted in the degradation substantial levels.					
36258114	4	86	theme	OMW	968:970	arg1	compound					972:979	OMW compound's	968:981	OMW compound's degradation	968:993	The catalytic findings have shown that [GTA-(PDA-g-DAC)@Fe3O4] exhibited good properties for OMW compound's degradation.					
36258114	0	87	theme	organic	80:86	arg1	matter					88:93	olive-oil mill wastewater organic matter	54:93	olive-oil mill wastewater organic matter	54:93	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked developed cellulose.					
36258114	1	88	theme	thermogravimetric	429:445	arg1	TGA					457:459	TGA	457:459	TGA	457:459	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	1	88	theme	thermogravimetric	429:445	arg1	analysis					447:454	thermogravimetric analysis	429:454	thermogravimetric analysis (TGA)	429:460	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	6	89	theme	OMW	1330:1332	arg1	solution					1334:1341	aqueous OMW solution	1322:1341	aqueous OMW solution	1322:1341	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	6	90	theme	mM	1188:1189	arg1	H2O2					1180:1183	H2O2	1180:1183	H2O2 10 mM	1180:1189	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	2	91	theme	magnetic	640:647	arg1	g/L					710:712	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L)	636:713	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2	636:727	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	5	92	theme	substantial	1086:1096	arg1	levels					1098:1103	the degradation substantial levels	1070:1103	the degradation substantial levels	1070:1103	The sonocatalytic process coupling and extra oxidant addition resulted in the degradation substantial levels.					
36258114	0	93	theme	magnetic	102:109	arg1	glutarlaldehyde-crosslinked					111:137	new magnetic glutarlaldehyde-crosslinked	98:137	new magnetic glutarlaldehyde-crosslinked	98:137	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked developed cellulose.					
36258114	1	94	theme	infrared	374:381	arg1	spectroscopy					390:401	infrared (FTIR) spectroscopy	374:401	infrared (FTIR) spectroscopy	374:401	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	7	95	theme	amino	1482:1486	arg1	compounds					1488:1496	total amino compounds	1476:1496	total amino compounds (TAC)	1476:1502	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	7	95	theme	amino	1482:1486	arg1	TAC					1499:1501	TAC	1499:1501	TAC	1499:1501	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	6	96	theme	10	1185:1186	arg1	mM					1188:1189	mM	1188:1189	mM	1188:1189	For instance, the concomitant effect of degradation optimized parameters; H2O2 10 mM, [GTA-(PDA-g-DAC) @Fe3O4] nanocomposites 2.5 g/L, at pH 3, and T 35 °C for 70 min resulted in an almost complete mineralization of aqueous OMW solution followed by a significant decolorization.					
36258114	9	97	theme	catalytic	1810:1818	arg1	technology					1860:1869	a promising treatment technology	1838:1869	a promising treatment technology for OMW effluents	1838:1887	The gathered findings gave the evidence that sono-heterogeneous catalytic Fenton process is a promising treatment technology for OMW effluents.					
36258114	9	97	theme	catalytic	1810:1818	arg1	process					1827:1833	sono-heterogeneous catalytic Fenton process	1791:1833	sono-heterogeneous catalytic Fenton process	1791:1833	The gathered findings gave the evidence that sono-heterogeneous catalytic Fenton process is a promising treatment technology for OMW effluents.					
36258114	1	98	theme	analysis	531:538	arg1	diffraction					410:420	X-ray diffraction	404:420	X-ray diffraction (XRD)	404:426	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	1	98	theme	analysis	531:538	arg1	analysis					447:454	thermogravimetric analysis	429:454	thermogravimetric analysis (TGA)	429:460	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	1	98	theme	analysis	531:538	arg1	TGA					457:459	TGA	457:459	TGA	457:459	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	1	98	theme	analysis	531:538	arg1	XRD					423:425	XRD	423:425	XRD	423:425	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	1	98	theme	analysis	531:538	arg1	spectroscopy					390:401	infrared (FTIR) spectroscopy	374:401	infrared (FTIR) spectroscopy	374:401	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	1	98	theme	analysis	531:538	arg1	point					501:505	point	501:505	point of zero charge (pH pzc) analysis	501:538	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	1	98	theme	analysis	531:538	arg1	measurements					483:494	magnetic properties measurements	463:494	magnetic properties measurements	463:494	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	0	99	theme	optimized	3:11	arg1	degradation					39:49	An optimized sono-heterogeneous Fenton degradation	0:49	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked	0:137	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked developed cellulose.					
36258114	2	100	theme	catalyst	661:668	arg1	g/L					710:712	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L)	636:713	the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2	636:727	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	0	101	theme	Fenton	32:37	arg1	degradation					39:49	An optimized sono-heterogeneous Fenton degradation	0:49	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked	0:137	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked developed cellulose.					
36258114	1	102	theme	PDA-g-DAC	316:324	arg1	Fe3O4					328:332	new designed [GTA-(PDA-g-DAC) @Fe3O4]	297:333	new designed [GTA-(PDA-g-DAC) @Fe3O4]	297:333	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36258114	5	103	theme	sonocatalytic	1000:1012	arg1	process					1014:1020	The sonocatalytic process coupling and extra oxidant addition	996:1056	The sonocatalytic process coupling and extra oxidant addition	996:1056	The sonocatalytic process coupling and extra oxidant addition resulted in the degradation substantial levels.					
36258114	7	104	theme	catalytic	1638:1646	arg1	process					1655:1661	the optimized sono-heterogeneous catalytic Fenton process	1605:1661	the optimized sono-heterogeneous catalytic Fenton process	1605:1661	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	7	105	theme	total	1444:1448	arg1	TPC					1470:1472	TPC	1470:1472	TPC	1470:1472	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	7	105	theme	total	1444:1448	arg1	compounds					1459:1467	total phenolic compounds	1444:1467	total phenolic compounds (TPC)	1444:1473	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	9	106	theme	treatment	1850:1858	arg1	technology					1860:1869	a promising treatment technology	1838:1869	a promising treatment technology for OMW effluents	1838:1887	The gathered findings gave the evidence that sono-heterogeneous catalytic Fenton process is a promising treatment technology for OMW effluents.					
36258114	9	106	theme	treatment	1850:1858	arg1	process					1827:1833	sono-heterogeneous catalytic Fenton process	1791:1833	sono-heterogeneous catalytic Fenton process	1791:1833	The gathered findings gave the evidence that sono-heterogeneous catalytic Fenton process is a promising treatment technology for OMW effluents.					
36258114	0	107	theme	mill	64:67	arg1	matter					88:93	olive-oil mill wastewater organic matter	54:93	olive-oil mill wastewater organic matter	54:93	An optimized sono-heterogeneous Fenton degradation of olive-oil mill wastewater organic matter by new magnetic glutarlaldehyde-crosslinked developed cellulose.					
36258114	7	108	theme	optimized	1609:1617	arg1	process					1655:1661	the optimized sono-heterogeneous catalytic Fenton process	1605:1661	the optimized sono-heterogeneous catalytic Fenton process	1605:1661	Oxidation results exhibited efficient degradation rates in total phenolic compounds (TPC), total amino compounds (TAC), and chemical oxygen demand (COD) oxidation rate were 89.88, 92.75, and 95.66 respectively following the optimized sono-heterogeneous catalytic Fenton process.					
36258114	2	109	theme	ultrasonic	753:762	arg1	power					764:768	500WL-1 ultrasonic power	745:768	500WL-1 ultrasonic power (US)	745:773	A preliminary removal study showed significant degradation efficiency (75%) occurred combining the magnetic synthesized catalyst [GTA-(PDA-g-DAC)@Fe3O4] ([catalyst] = 2 g/L) with US /H2O2 and maintaining 500WL-1 ultrasonic power (US).					
36258114	1	110	theme	sono-heterogeneous	257:274	arg1	process					283:289	a sono-heterogeneous Fenton process	255:289	a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis	255:538	The present study highlights the olive mill wastewater (OMW) treatment characteristics through a sono-heterogeneous Fenton process using new designed [GTA-(PDA-g-DAC) @Fe3O4] and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), magnetic properties measurements, and point of zero charge (pH pzc) analysis.					
36343400	10	0	theme	microorganisms	1699:1712	arg1	responses					1662:1670	responses	1662:1670	responses of β-glucosidase-producing microorganisms to cellulose degradation	1662:1737	The above results indicated that responses of β-glucosidase-producing microorganisms to cellulose degradation were reflected at both network and individual levels and multi-interaction analysis could better explain the relationship between variables concerning composting cellulose degradation.					
36343400	8	1	with	cellulose	1291:1299	arg1	Streptomyces					1306:1317	Streptomyces	1306:1317	Streptomyces (0.3045**)	1306:1328	β-Glucosidase-producing bacteria and fungi worked synergistically as a network to degrade cellulose with Streptomyces (0.3045**) as the key community revealed by multi-interaction analysis.					
36343400	8	1	with	cellulose	1291:1299	arg1	**					1326:1327	0.3045**	1320:1327	0.3045**	1320:1327	β-Glucosidase-producing bacteria and fungi worked synergistically as a network to degrade cellulose with Streptomyces (0.3045**) as the key community revealed by multi-interaction analysis.					
36343400	5	2	theme	matter	716:721	arg1	content					723:729	organic matter content	708:729	organic matter content of 37.83%	708:739	Results showed that the compost was mature with organic matter content of 37.83% and pH value of 7.36 and pure corn straw could be composted successfully.					
36343400	1	3	theme	Poor	192:195	arg1	management					197:206	Poor management	192:206	Poor management of crop residues	192:223	Poor management of crop residues leads to environmental pollution and composting is a sustainable practice for addressing the challenge.					
36343400	0	4	theme	cellulose	102:110	arg1	degradation					112:122	cellulose degradation	102:122	cellulose degradation during composting of pure corn straw by multi-interaction analysis	102:189	Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis.					
36343400	6	5	with	degradation	860:870	arg1	476.25 μmol·p-Nitr/kg·dw·min					913:940	476.25 μmol·p-Nitr/kg·dw·min	913:940	476.25 μmol·p-Nitr/kg·dw·min	913:940	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	6	5	with	degradation	860:870	arg1	activity					903:910	the highest β-glucosidase activity	877:910	the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min)	877:941	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	6	5	with	degradation	860:870	arg1	diversity					957:965	microbial diversity	947:965	microbial diversity (Shannon index, 3.63; Chao1 index, 500.81)	947:1008	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	7	6	theme	Significant	1011:1021	arg1	succession					1037:1046	Significant compositional succession	1011:1046	Significant compositional succession	1011:1046	Significant compositional succession was observed in the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera.					
36343400	4	7	theme	high-throughput	632:646	arg1	sequencing					648:657	high-throughput sequencing	632:657	high-throughput sequencing	632:657	Community structure of β-glucosidase-producing microorganisms during composting was deciphered using high-throughput sequencing.					
36343400	5	8	theme	corn	771:774	arg1	straw					776:780	pure corn straw	766:780	pure corn straw	766:780	Results showed that the compost was mature with organic matter content of 37.83% and pH value of 7.36 and pure corn straw could be composted successfully.					
36343400	4	9	theme	Community	531:539	arg1	structure					541:549	Community structure	531:549	Community structure of β-glucosidase-producing microorganisms during composting	531:609	Community structure of β-glucosidase-producing microorganisms during composting was deciphered using high-throughput sequencing.					
36343400	6	10	theme	Chao1	989:993	arg1	index					995:999	Chao1 index	989:999	Chao1 index	989:999	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	7	11	theme	compositional	1023:1035	arg1	succession					1037:1046	Significant compositional succession	1011:1046	Significant compositional succession	1011:1046	Significant compositional succession was observed in the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera.					
36343400	7	12	with	communities	1079:1089	arg1	Agromyces					1162:1170	Agromyces	1162:1170	Agromyces (11.68%)	1162:1179	Significant compositional succession was observed in the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera.					
36343400	7	12	with	communities	1079:1089	arg1	Streptomyces					1114:1125	Streptomyces	1114:1125	Streptomyces (14.32%)	1114:1134	Significant compositional succession was observed in the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera.					
36343400	7	12	with	communities	1079:1089	arg1	Trichoderma					1137:1147	Trichoderma	1137:1147	Trichoderma (13.85%)	1137:1156	Significant compositional succession was observed in the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera.					
36343400	7	12	with	communities	1079:1089	arg1	%					1155:1155	13.85%	1150:1155	13.85%	1150:1155	Significant compositional succession was observed in the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera.					
36343400	7	12	with	communities	1079:1089	arg1	%					1178:1178	11.68%	1173:1178	11.68%	1173:1178	Significant compositional succession was observed in the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera.					
36343400	7	12	with	communities	1079:1089	arg1	%					1133:1133	14.32%	1128:1133	14.32%	1128:1133	Significant compositional succession was observed in the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera.					
36343400	5	13	theme	straw	776:780	arg1	value					748:752	pH value	745:752	pH value of 7.36 and pure corn straw	745:780	Results showed that the compost was mature with organic matter content of 37.83% and pH value of 7.36 and pure corn straw could be composted successfully.					
36343400	5	14	theme	7.36	757:760	arg1	value					748:752	pH value	745:752	pH value of 7.36 and pure corn straw	745:780	Results showed that the compost was mature with organic matter content of 37.83% and pH value of 7.36 and pure corn straw could be composted successfully.					
36343400	11	15	theme	microbial	1992:2000	arg1	communities					2002:2012	cellulose degradation microbial communities	1970:2012	cellulose degradation microbial communities	1970:2012	The work is of significance for understanding cellulose degradation microbial communities and process during composting of pure corn straw.					
36343400	6	16	theme	cellulose	850:858	arg1	degradation					860:870	cellulose degradation	850:870	cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81)	850:1008	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	8	17	theme	multi-interaction	1363:1379	arg1	analysis					1381:1388	multi-interaction analysis	1363:1388	multi-interaction analysis	1363:1388	β-Glucosidase-producing bacteria and fungi worked synergistically as a network to degrade cellulose with Streptomyces (0.3045**) as the key community revealed by multi-interaction analysis.					
36343400	2	18	with	composting	354:363	arg1	straw					380:384	pure crop straw	370:384	pure crop straw	370:384	However, knowledge about composting with pure crop straw is still limited, which is a novel and feasible composting strategy.					
36343400	0	19	theme	pure	145:148	arg1	straw					155:159	pure corn straw	145:159	pure corn straw	145:159	Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis.					
36343400	8	20	theme	β-Glucosidase-producing	1201:1223	arg1	bacteria					1225:1232	β-Glucosidase-producing bacteria	1201:1232	β-Glucosidase-producing bacteria	1201:1232	β-Glucosidase-producing bacteria and fungi worked synergistically as a network to degrade cellulose with Streptomyces (0.3045**) as the key community revealed by multi-interaction analysis.					
36343400	11	21	theme	pure	2047:2050	arg1	straw					2057:2061	pure corn straw	2047:2061	pure corn straw	2047:2061	The work is of significance for understanding cellulose degradation microbial communities and process during composting of pure corn straw.					
36343400	9	22	theme	environmental	1455:1467	arg1	parameters					1469:1478	key environmental parameters	1451:1478	key environmental parameters regulating cellulose degradation via influencing β-glucosidase-producing communities	1451:1563	Organic matter (-0.415***) and temperature (-0.327***) were key environmental parameters regulating cellulose degradation via influencing β-glucosidase-producing communities, and β-glucosidase played a key role in mediating this process.					
36343400	9	23	theme	key	1451:1453	arg1	parameters					1469:1478	key environmental parameters	1451:1478	key environmental parameters regulating cellulose degradation via influencing β-glucosidase-producing communities	1451:1563	Organic matter (-0.415***) and temperature (-0.327***) were key environmental parameters regulating cellulose degradation via influencing β-glucosidase-producing communities, and β-glucosidase played a key role in mediating this process.					
36343400	6	24	theme	major	833:837	arg1	period					839:844	major period	833:844	major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81)	833:1008	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	1	25	theme	crop	211:214	arg1	residues					216:223	crop residues	211:223	crop residues	211:223	Poor management of crop residues leads to environmental pollution and composting is a sustainable practice for addressing the challenge.					
36343400	9	26	theme	key	1593:1595	arg1	role					1597:1600	a key role	1591:1600	a key role	1591:1600	Organic matter (-0.415***) and temperature (-0.327***) were key environmental parameters regulating cellulose degradation via influencing β-glucosidase-producing communities, and β-glucosidase played a key role in mediating this process.					
36343400	6	27	theme	microbial	947:955	arg1	diversity					957:965	microbial diversity	947:965	microbial diversity (Shannon index, 3.63; Chao1 index, 500.81)	947:1008	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	10	28	theme	above	1633:1637	arg1	results					1639:1645	The above results	1629:1645	The above results	1629:1645	The above results indicated that responses of β-glucosidase-producing microorganisms to cellulose degradation were reflected at both network and individual levels and multi-interaction analysis could better explain the relationship between variables concerning composting cellulose degradation.					
36343400	3	29	from	composted	498:506	arg1	study					463:467	this study	458:467	this study	458:467	In this study, pure corn straw was in-situ composted for better management.					
36343400	1	30	theme	residues	216:223	arg1	management					197:206	Poor management	192:206	Poor management of crop residues	192:223	Poor management of crop residues leads to environmental pollution and composting is a sustainable practice for addressing the challenge.					
36343400	5	31	theme	organic	708:714	arg1	content					723:729	organic matter content	708:729	organic matter content of 37.83%	708:739	Results showed that the compost was mature with organic matter content of 37.83% and pH value of 7.36 and pure corn straw could be composted successfully.					
36343400	6	32	dep	index	976:980	arg1	index					995:999	Chao1 index	989:999	Chao1 index	989:999	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	9	33	theme	cellulose	1491:1499	arg1	degradation					1501:1511	cellulose degradation	1491:1511	cellulose degradation	1491:1511	Organic matter (-0.415***) and temperature (-0.327***) were key environmental parameters regulating cellulose degradation via influencing β-glucosidase-producing communities, and β-glucosidase played a key role in mediating this process.					
36343400	0	34	theme	microorganisms	39:52	arg1	Exploration					0:10	Exploration	0:10	Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis.	0:190	Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis.					
36343400	8	35	theme	key	1337:1339	arg1	cellulose					1291:1299	cellulose	1291:1299	cellulose with Streptomyces (0.3045**)	1291:1328	β-Glucosidase-producing bacteria and fungi worked synergistically as a network to degrade cellulose with Streptomyces (0.3045**) as the key community revealed by multi-interaction analysis.					
36343400	8	35	theme	key	1337:1339	arg1	community					1341:1349	the key community	1333:1349	the key community revealed by multi-interaction analysis	1333:1388	β-Glucosidase-producing bacteria and fungi worked synergistically as a network to degrade cellulose with Streptomyces (0.3045**) as the key community revealed by multi-interaction analysis.					
36343400	0	36	theme	straw	155:159	arg1	composting					131:140	composting	131:140	composting of pure corn straw by multi-interaction analysis	131:189	Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis.					
36343400	10	37	theme	cellulose	1901:1909	arg1	degradation					1911:1921	composting cellulose degradation	1890:1921	composting cellulose degradation	1890:1921	The above results indicated that responses of β-glucosidase-producing microorganisms to cellulose degradation were reflected at both network and individual levels and multi-interaction analysis could better explain the relationship between variables concerning composting cellulose degradation.					
36343400	10	38	theme	individual	1774:1783	arg1	levels					1785:1790	individual levels	1774:1790	individual levels	1774:1790	The above results indicated that responses of β-glucosidase-producing microorganisms to cellulose degradation were reflected at both network and individual levels and multi-interaction analysis could better explain the relationship between variables concerning composting cellulose degradation.					
36343400	0	39	theme	β-glucosidase-producing	15:37	arg1	microorganisms					39:52	β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis	15:189	β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis	15:189	Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis.					
36343400	9	40	theme	Organic	1391:1397	arg1	matter					1399:1404	Organic matter	1391:1404	Organic matter (-0.415***)	1391:1416	Organic matter (-0.415***) and temperature (-0.327***) were key environmental parameters regulating cellulose degradation via influencing β-glucosidase-producing communities, and β-glucosidase played a key role in mediating this process.					
36343400	9	40	theme	Organic	1391:1397	arg1	***					1413:1415	-0.415***	1407:1415	-0.415***	1407:1415	Organic matter (-0.415***) and temperature (-0.327***) were key environmental parameters regulating cellulose degradation via influencing β-glucosidase-producing communities, and β-glucosidase played a key role in mediating this process.					
36343400	0	41	theme	corn	150:153	arg1	straw					155:159	pure corn straw	145:159	pure corn straw	145:159	Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis.					
36343400	10	42	theme	composting	1890:1899	arg1	degradation					1911:1921	composting cellulose degradation	1890:1921	composting cellulose degradation	1890:1921	The above results indicated that responses of β-glucosidase-producing microorganisms to cellulose degradation were reflected at both network and individual levels and multi-interaction analysis could better explain the relationship between variables concerning composting cellulose degradation.					
36343400	1	43	theme	environmental	234:246	arg1	pollution					248:256	environmental pollution	234:256	environmental pollution	234:256	Poor management of crop residues leads to environmental pollution and composting is a sustainable practice for addressing the challenge.					
36343400	10	44	theme	cellulose	1717:1725	arg1	degradation					1727:1737	cellulose degradation	1717:1737	cellulose degradation	1717:1737	The above results indicated that responses of β-glucosidase-producing microorganisms to cellulose degradation were reflected at both network and individual levels and multi-interaction analysis could better explain the relationship between variables concerning composting cellulose degradation.					
36343400	10	45	theme	multi-interaction	1796:1812	arg1	analysis					1814:1821	multi-interaction analysis	1796:1821	multi-interaction analysis	1796:1821	The above results indicated that responses of β-glucosidase-producing microorganisms to cellulose degradation were reflected at both network and individual levels and multi-interaction analysis could better explain the relationship between variables concerning composting cellulose degradation.					
36343400	0	46	theme	multi-interaction	164:180	arg1	analysis					182:189	multi-interaction analysis	164:189	multi-interaction analysis	164:189	Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis.					
36343400	3	47	theme	pure	470:473	arg1	straw					480:484	pure corn straw	470:484	pure corn straw	470:484	In this study, pure corn straw was in-situ composted for better management.					
36343400	11	48	theme	corn	2052:2055	arg1	straw					2057:2061	pure corn straw	2047:2061	pure corn straw	2047:2061	The work is of significance for understanding cellulose degradation microbial communities and process during composting of pure corn straw.					
36343400	2	49	theme	composting	434:443	arg1	strategy					445:452	a novel and feasible composting strategy	413:452	a novel and feasible composting strategy	413:452	However, knowledge about composting with pure crop straw is still limited, which is a novel and feasible composting strategy.					
36343400	2	49	theme	composting	434:443	arg1	which					404:408	which	404:408	which	404:408	However, knowledge about composting with pure crop straw is still limited, which is a novel and feasible composting strategy.					
36343400	0	50	theme	community	54:62	arg1	structure					64:72	community structure	54:72	community structure	54:72	Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis.					
36343400	4	51	theme	β-glucosidase-producing	554:576	arg1	microorganisms					578:591	β-glucosidase-producing microorganisms	554:591	β-glucosidase-producing microorganisms	554:591	Community structure of β-glucosidase-producing microorganisms during composting was deciphered using high-throughput sequencing.					
36343400	5	52	theme	pure	766:769	arg1	straw					776:780	pure corn straw	766:780	pure corn straw	766:780	Results showed that the compost was mature with organic matter content of 37.83% and pH value of 7.36 and pure corn straw could be composted successfully.					
36343400	7	53	theme	dominant	1184:1191	arg1	genera					1193:1198	dominant genera	1184:1198	dominant genera	1184:1198	Significant compositional succession was observed in the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera.					
36343400	6	54	theme	Cooling	815:821	arg1	phase					823:827	Cooling phase	815:827	Cooling phase	815:827	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	2	55	theme	feasible	425:432	arg1	strategy					445:452	a novel and feasible composting strategy	413:452	a novel and feasible composting strategy	413:452	However, knowledge about composting with pure crop straw is still limited, which is a novel and feasible composting strategy.					
36343400	2	55	theme	feasible	425:432	arg1	which					404:408	which	404:408	which	404:408	However, knowledge about composting with pure crop straw is still limited, which is a novel and feasible composting strategy.					
36343400	0	56	theme	key	78:80	arg1	communities					82:92	key communities	78:92	key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis	78:189	Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis.					
36343400	11	57	theme	cellulose	1970:1978	arg1	communities					2002:2012	cellulose degradation microbial communities	1970:2012	cellulose degradation microbial communities	1970:2012	The work is of significance for understanding cellulose degradation microbial communities and process during composting of pure corn straw.					
36343400	5	58	theme	%	739:739	arg1	content					723:729	organic matter content	708:729	organic matter content of 37.83%	708:739	Results showed that the compost was mature with organic matter content of 37.83% and pH value of 7.36 and pure corn straw could be composted successfully.					
36343400	10	59	theme	β-glucosidase-producing	1675:1697	arg1	microorganisms					1699:1712	β-glucosidase-producing microorganisms	1675:1712	β-glucosidase-producing microorganisms	1675:1712	The above results indicated that responses of β-glucosidase-producing microorganisms to cellulose degradation were reflected at both network and individual levels and multi-interaction analysis could better explain the relationship between variables concerning composting cellulose degradation.					
36343400	3	60	theme	better	512:517	arg1	management					519:528	better management	512:528	better management	512:528	In this study, pure corn straw was in-situ composted for better management.					
36343400	4	61	theme	microorganisms	578:591	arg1	structure					541:549	Community structure	531:549	Community structure of β-glucosidase-producing microorganisms during composting	531:609	Community structure of β-glucosidase-producing microorganisms during composting was deciphered using high-throughput sequencing.					
36343400	5	62	theme	pH	745:746	arg1	value					748:752	pH value	745:752	pH value of 7.36 and pure corn straw	745:780	Results showed that the compost was mature with organic matter content of 37.83% and pH value of 7.36 and pure corn straw could be composted successfully.					
36343400	6	63	theme	β-glucosidase	889:901	arg1	476.25 μmol·p-Nitr/kg·dw·min					913:940	476.25 μmol·p-Nitr/kg·dw·min	913:940	476.25 μmol·p-Nitr/kg·dw·min	913:940	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	6	63	theme	β-glucosidase	889:901	arg1	activity					903:910	the highest β-glucosidase activity	877:910	the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min)	877:941	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	0	64	dep	microorganisms	39:52	arg1	structure					64:72	community structure	54:72	community structure	54:72	Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis.					
36343400	0	64	dep	microorganisms	39:52	arg1	communities					82:92	key communities	78:92	key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis	78:189	Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis.					
36343400	7	65	theme	functional	1068:1077	arg1	communities					1079:1089	the functional communities	1064:1089	the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera	1064:1198	Significant compositional succession was observed in the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera.					
36343400	2	66	theme	crop	375:378	arg1	straw					380:384	pure crop straw	370:384	pure crop straw	370:384	However, knowledge about composting with pure crop straw is still limited, which is a novel and feasible composting strategy.					
36343400	6	67	dep	diversity	957:965	arg1	index					976:980	index	976:980	index	976:980	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	6	68	theme	highest	881:887	arg1	476.25 μmol·p-Nitr/kg·dw·min					913:940	476.25 μmol·p-Nitr/kg·dw·min	913:940	476.25 μmol·p-Nitr/kg·dw·min	913:940	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	6	68	theme	highest	881:887	arg1	activity					903:910	the highest β-glucosidase activity	877:910	the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min)	877:941	Cooling phase was major period for cellulose degradation with the highest β-glucosidase activity (476.25 μmol·p-Nitr/kg·dw·min) and microbial diversity (Shannon index, 3.63; Chao1 index, 500.81).					
36343400	2	69	theme	pure	370:373	arg1	straw					380:384	pure crop straw	370:384	pure crop straw	370:384	However, knowledge about composting with pure crop straw is still limited, which is a novel and feasible composting strategy.					
36343400	3	70	theme	in-situ	490:496	arg1	composted					498:506	in-situ composted	490:506	in-situ composted for better management	490:528	In this study, pure corn straw was in-situ composted for better management.					
36343400	9	71	theme	β-glucosidase-producing	1529:1551	arg1	communities					1553:1563	β-glucosidase-producing communities	1529:1563	β-glucosidase-producing communities	1529:1563	Organic matter (-0.415***) and temperature (-0.327***) were key environmental parameters regulating cellulose degradation via influencing β-glucosidase-producing communities, and β-glucosidase played a key role in mediating this process.					
36343400	3	72	theme	corn	475:478	arg1	straw					480:484	pure corn straw	470:484	pure corn straw	470:484	In this study, pure corn straw was in-situ composted for better management.					
36343400	2	73	theme	novel	415:419	arg1	strategy					445:452	a novel and feasible composting strategy	413:452	a novel and feasible composting strategy	413:452	However, knowledge about composting with pure crop straw is still limited, which is a novel and feasible composting strategy.					
36343400	2	73	theme	novel	415:419	arg1	which					404:408	which	404:408	which	404:408	However, knowledge about composting with pure crop straw is still limited, which is a novel and feasible composting strategy.					
36343400	11	74	theme	straw	2057:2061	arg1	composting					2033:2042	composting	2033:2042	composting of pure corn straw	2033:2061	The work is of significance for understanding cellulose degradation microbial communities and process during composting of pure corn straw.					
36343400	7	75	located	observed	1052:1059	arg2	succession					1037:1046	Significant compositional succession	1011:1046	Significant compositional succession	1011:1046	Significant compositional succession was observed in the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera.					
36343400	7	75	located	observed	1052:1059	arg1	communities					1079:1089	the functional communities	1064:1089	the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera	1064:1198	Significant compositional succession was observed in the functional communities during composting with Streptomyces (14.32%), Trichoderma (13.85%) and Agromyces (11.68%) as dominant genera.					
36343400	5	76	with	mature	696:701	arg1	content					723:729	organic matter content	708:729	organic matter content of 37.83%	708:739	Results showed that the compost was mature with organic matter content of 37.83% and pH value of 7.36 and pure corn straw could be composted successfully.					
36343400	11	77	theme	degradation	1980:1990	arg1	communities					2002:2012	cellulose degradation microbial communities	1970:2012	cellulose degradation microbial communities	1970:2012	The work is of significance for understanding cellulose degradation microbial communities and process during composting of pure corn straw.					
36343400	1	78	theme	sustainable	278:288	arg1	composting					262:271	composting	262:271	composting	262:271	Poor management of crop residues leads to environmental pollution and composting is a sustainable practice for addressing the challenge.					
36343400	1	78	theme	sustainable	278:288	arg1	practice					290:297	a sustainable practice	276:297	a sustainable practice for addressing the challenge	276:326	Poor management of crop residues leads to environmental pollution and composting is a sustainable practice for addressing the challenge.					
35753031	3	0	contain	have	555:558	arg1	flours					548:553	RESULTS Andean flours	533:553	RESULTS Andean flours	533:553	RESULTS Andean flours have high protein, fiber, and fat content, and display high water and oil absorption.					
35753031	3	0	contain	have	555:558	arg2	fiber					574:578	high protein, fiber, and fat content	560:595	fiber	574:578	RESULTS Andean flours have high protein, fiber, and fat content, and display high water and oil absorption.					
35753031	3	0	contain	have	555:558	arg2	content					589:595	high protein, fiber, and fat content	560:595	content	589:595	RESULTS Andean flours have high protein, fiber, and fat content, and display high water and oil absorption.					
35753031	10	1	theme	tarwi	1598:1602	arg1	flours					1615:1620	tarwi and kañiwa flours	1598:1620	tarwi and kañiwa flours	1598:1620	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	1	2	theme	excellent	219:227	arg1	profile					241:247	an excellent nutritional profile	216:247	an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations	216:302	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	5	3	contain	had	891:893	arg1	batters					854:860	the batters	850:860	the batters formulated with these flours	850:889	Depending on their ability to interact with water, the batters formulated with these flours had a higher consistency.					
35753031	5	3	contain	had	891:893	arg2	consistency					904:914	a higher consistency	895:914	a higher consistency	895:914	Depending on their ability to interact with water, the batters formulated with these flours had a higher consistency.					
35753031	1	4	from	inclusion	253:261	arg1	formulations					291:302	vegan gluten-free muffin formulations	266:302	vegan gluten-free muffin formulations	266:302	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	7	5	theme	Andean	1112:1117	arg1	flours					1119:1124	Andean flours	1112:1124	Andean flours	1112:1124	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	4	6	theme	lower	773:777	arg1	availability					785:796	lower water availability	773:796	lower water availability	773:796	In premixes formulated with potato starch and Andean flours, a reduction in paste viscosity was observed due to starch dilution and lower water availability.					
35753031	7	7	theme	kañiwa	1271:1276	arg1	%					1281:1281	25%	1279:1281	25%	1279:1281	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	7	7	theme	kañiwa	1271:1276	arg1	flours					1284:1289	tarwi (25%) and kañiwa (25%) flours	1255:1289	flours	1284:1289	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	9	8	theme	such	1495:1498	arg1	products					1500:1507	such products	1495:1507	such products	1495:1507	These color changes are desirable in gluten-free products because such products are frequently pale due to their high starch content.					
35753031	6	9	theme	fiber	1077:1081	arg1	fragments					1083:1091	fiber fragments	1077:1091	fiber fragments	1077:1091	Confocal laser scanning micrographs showed that batters with Andean flours presented a complex matrix with dispersed starch granules surrounded by proteins and fiber fragments.					
35753031	10	10	theme	kañiwa	1608:1613	arg1	flours					1615:1620	tarwi and kañiwa flours	1598:1620	tarwi and kañiwa flours	1598:1620	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	9	11	theme	starch	1547:1552	arg1	content					1554:1560	their high starch content	1536:1560	their high starch content	1536:1560	These color changes are desirable in gluten-free products because such products are frequently pale due to their high starch content.					
35753031	11	12	theme	Chemical	1813:1820	arg1	Industry					1822:1829	Chemical Industry	1813:1829	Chemical Industry	1813:1829	© 2022 Society of Chemical Industry.					
35753031	8	13	theme	intrinsic	1296:1304	arg1	color					1306:1310	The intrinsic color	1292:1310	The intrinsic color of these flours	1292:1326	The intrinsic color of these flours modified crumb color, and their reducing sugar content also favored Maillard reactions in the crust.					
35753031	7	14	theme	tarwi	1255:1259	arg1	mixture					1244:1250	a mixture	1242:1250	a mixture of tarwi (25%) and kañiwa (25%) flours	1242:1289	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	7	14	theme	tarwi	1255:1259	arg1	flour					1226:1230	tarwi flour	1220:1230	tarwi flour (50%)	1220:1236	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	7	14	theme	tarwi	1255:1259	arg1	%					1235:1235	50%	1233:1235	50%	1233:1235	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	3	15	theme	Andean	541:546	arg1	flours					548:553	RESULTS Andean flours	533:553	RESULTS Andean flours	533:553	RESULTS Andean flours have high protein, fiber, and fat content, and display high water and oil absorption.					
35753031	8	16	theme	flours	1321:1326	arg1	color					1306:1310	The intrinsic color	1292:1310	The intrinsic color of these flours	1292:1326	The intrinsic color of these flours modified crumb color, and their reducing sugar content also favored Maillard reactions in the crust.					
35753031	7	17	theme	tarwi	1220:1224	arg1	flour					1226:1230	tarwi flour	1220:1230	tarwi flour (50%)	1220:1236	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	7	17	theme	tarwi	1220:1224	arg1	%					1235:1235	50%	1233:1235	50%	1233:1235	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	3	18	theme	protein	565:571	arg1	fiber					574:578	high protein, fiber, and fat content	560:595	fiber	574:578	RESULTS Andean flours have high protein, fiber, and fat content, and display high water and oil absorption.					
35753031	1	19	theme	Andean	122:127	arg1	kañiwa					166:171	kañiwa	166:171	kañiwa (Chenopodium pallidicaule Aellen)	166:205	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	1	19	theme	Andean	122:127	arg1	tarwi					136:140	tarwi	136:140	tarwi (Lupinus mutabilis)	136:160	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	1	19	theme	Andean	122:127	arg1	crops					129:133	two Andean crops	118:133	two Andean crops	118:133	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	6	20	theme	laser	926:930	arg1	micrographs					941:951	Confocal laser scanning micrographs	917:951	Confocal laser scanning micrographs	917:951	Confocal laser scanning micrographs showed that batters with Andean flours presented a complex matrix with dispersed starch granules surrounded by proteins and fiber fragments.					
35753031	2	21	theme	muffins	451:457	arg1	quality					428:434	the technological quality	410:434	the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours	410:515	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	2	21	theme	muffins	451:457	arg1	flours					398:403	tarwi and kañiwa flours	381:403	tarwi and kañiwa flours	381:403	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	8	22	theme	crumb	1337:1341	arg1	color					1343:1347	crumb color	1337:1347	crumb color	1337:1347	The intrinsic color of these flours modified crumb color, and their reducing sugar content also favored Maillard reactions in the crust.					
35753031	7	23	theme	specific	1147:1154	arg1	volume					1156:1161	a slightly lower specific volume	1130:1161	a slightly lower specific volume	1130:1161	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	10	24	theme	improved	1711:1718	arg1	profile					1732:1738	improved nutritional profile	1711:1738	improved nutritional profile	1711:1738	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	10	25	dep	CONCLUSIONS	1563:1573	arg1	showed					1586:1591	showed	1586:1591	showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours	1586:1792	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	0	26	theme	vegan	66:70	arg1	muffins					84:90	vegan gluten-free muffins	66:90	vegan gluten-free muffins	66:90	Andean crops: kañiwa and tarwi flours used for the development of vegan gluten-free muffins.					
35753031	1	27	dep	BACKGROUND	93:102	arg1	present					208:214	present	208:214	present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations	208:302	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	0	28	theme	muffins	84:90	arg1	development					51:61	the development	47:61	the development of vegan gluten-free muffins	47:90	Andean crops: kañiwa and tarwi flours used for the development of vegan gluten-free muffins.					
35753031	9	29	from	desirable	1453:1461	arg1	products					1478:1485	gluten-free products	1466:1485	gluten-free products	1466:1485	These color changes are desirable in gluten-free products because such products are frequently pale due to their high starch content.					
35753031	8	30	theme	sugar	1369:1373	arg1	content					1375:1381	their reducing sugar content	1354:1381	their reducing sugar content	1354:1381	The intrinsic color of these flours modified crumb color, and their reducing sugar content also favored Maillard reactions in the crust.					
35753031	4	31	theme	potato	669:674	arg1	starch					676:681	potato starch	669:681	potato starch	669:681	In premixes formulated with potato starch and Andean flours, a reduction in paste viscosity was observed due to starch dilution and lower water availability.					
35753031	0	32	theme	Andean	0:5	arg1	crops					7:11	Andean crops	0:11	Andean crops: kañiwa and tarwi flours used for the development of vegan gluten-free muffins.	0:91	Andean crops: kañiwa and tarwi flours used for the development of vegan gluten-free muffins.					
35753031	1	33	theme	nutritional	229:239	arg1	profile					241:247	an excellent nutritional profile	216:247	an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations	216:302	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	2	34	theme	kañiwa	391:396	arg1	flours					398:403	tarwi and kañiwa flours	381:403	tarwi and kañiwa flours	381:403	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	2	35	theme	batters	439:445	arg1	quality					428:434	the technological quality	410:434	the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours	410:515	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	2	35	theme	batters	439:445	arg1	flours					398:403	tarwi and kañiwa flours	381:403	tarwi and kañiwa flours	381:403	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	8	36	theme	Maillard	1396:1403	arg1	reactions					1405:1413	Maillard reactions	1396:1413	Maillard reactions in the crust	1396:1426	The intrinsic color of these flours modified crumb color, and their reducing sugar content also favored Maillard reactions in the crust.					
35753031	2	37	theme	tarwi	381:385	arg1	flours					398:403	tarwi and kañiwa flours	381:403	tarwi and kañiwa flours	381:403	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	9	38	theme	color	1435:1439	arg1	changes					1441:1447	These color changes	1429:1447	These color changes	1429:1447	These color changes are desirable in gluten-free products because such products are frequently pale due to their high starch content.					
35753031	2	39	theme	quality	428:434	arg1	composition					333:343	proximal composition	324:343	proximal composition	324:343	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	2	39	theme	quality	428:434	arg1	properties					367:376	techno-functional properties	349:376	techno-functional properties	349:376	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	10	40	theme	technological	1685:1697	arg1	quality					1699:1705	good technological quality	1680:1705	good technological quality	1680:1705	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	1	41	dep	tarwi	136:140	arg1	mutabilis					151:159	Lupinus mutabilis	143:159	Lupinus mutabilis	143:159	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	1	42	theme	gluten-free	272:282	arg1	formulations					291:302	vegan gluten-free muffin formulations	266:302	vegan gluten-free muffin formulations	266:302	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	6	43	with	batters	965:971	arg1	flours					985:990	Andean flours	978:990	Andean flours	978:990	Confocal laser scanning micrographs showed that batters with Andean flours presented a complex matrix with dispersed starch granules surrounded by proteins and fiber fragments.					
35753031	10	44	theme	gluten-free	1657:1667	arg1	vegan					1650:1654	vegan	1650:1654	vegan	1650:1654	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	10	44	theme	gluten-free	1657:1667	arg1	muffins					1669:1675	gluten-free muffins	1657:1675	gluten-free muffins of good technological quality and improved nutritional profile	1657:1738	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	3	45	theme	high	610:613	arg1	water					615:619	high water	610:619	high water	610:619	RESULTS Andean flours have high protein, fiber, and fat content, and display high water and oil absorption.					
35753031	1	46	theme	Chenopodium	174:184	arg1	Aellen					199:204	Chenopodium pallidicaule Aellen	174:204	Chenopodium pallidicaule Aellen	174:204	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	1	46	theme	Chenopodium	174:184	arg1	kañiwa					166:171	kañiwa	166:171	kañiwa (Chenopodium pallidicaule Aellen)	166:205	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	4	47	located	observed	737:744	arg2	reduction					704:712	a reduction	702:712	a reduction in paste viscosity	702:731	In premixes formulated with potato starch and Andean flours, a reduction in paste viscosity was observed due to starch dilution and lower water availability.					
35753031	4	47	located	observed	737:744	arg1	premixes					644:651	premixes	644:651	premixes formulated with potato starch and Andean flours	644:699	In premixes formulated with potato starch and Andean flours, a reduction in paste viscosity was observed due to starch dilution and lower water availability.					
35753031	10	48	theme	nutritional	1720:1730	arg1	profile					1732:1738	improved nutritional profile	1711:1738	improved nutritional profile	1711:1738	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	2	49	theme	proximal	324:331	arg1	composition					333:343	proximal composition	324:343	proximal composition	324:343	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	6	50	theme	dispersed	1024:1032	arg1	granules					1041:1048	dispersed starch granules	1024:1048	dispersed starch granules surrounded by proteins and fiber fragments	1024:1091	Confocal laser scanning micrographs showed that batters with Andean flours presented a complex matrix with dispersed starch granules surrounded by proteins and fiber fragments.					
35753031	8	51	from	reactions	1405:1413	arg1	crust					1422:1426	the crust	1418:1426	the crust	1418:1426	The intrinsic color of these flours modified crumb color, and their reducing sugar content also favored Maillard reactions in the crust.					
35753031	4	52	theme	water	779:783	arg1	availability					785:796	lower water availability	773:796	lower water availability	773:796	In premixes formulated with potato starch and Andean flours, a reduction in paste viscosity was observed due to starch dilution and lower water availability.					
35753031	7	53	theme	flours	1284:1289	arg1	mixture					1244:1250	a mixture	1242:1250	a mixture of tarwi (25%) and kañiwa (25%) flours	1242:1289	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	7	53	theme	flours	1284:1289	arg1	flour					1226:1230	tarwi flour	1220:1230	tarwi flour (50%)	1220:1236	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	7	53	theme	flours	1284:1289	arg1	%					1235:1235	50%	1233:1235	50%	1233:1235	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	4	54	theme	starch	753:758	arg1	dilution					760:767	starch dilution	753:767	starch dilution	753:767	In premixes formulated with potato starch and Andean flours, a reduction in paste viscosity was observed due to starch dilution and lower water availability.					
35753031	10	55	theme	ancestral	1777:1785	arg1	flours					1787:1792	these underutilized ancestral flours	1757:1792	these underutilized ancestral flours	1757:1792	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	9	56	theme	high	1542:1545	arg1	content					1554:1560	their high starch content	1536:1560	their high starch content	1536:1560	These color changes are desirable in gluten-free products because such products are frequently pale due to their high starch content.					
35753031	3	57	theme	RESULTS	533:539	arg1	flours					548:553	RESULTS Andean flours	533:553	RESULTS Andean flours	533:553	RESULTS Andean flours have high protein, fiber, and fat content, and display high water and oil absorption.					
35753031	7	58	mod	modified	1208:1215	arg1	hardness					1191:1198	crumb hardness	1185:1198	crumb hardness	1185:1198	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	7	58	mod	modified	1208:1215	arg3	mixture					1244:1250	a mixture	1242:1250	a mixture of tarwi (25%) and kañiwa (25%) flours	1242:1289	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	7	58	mod	modified	1208:1215	arg3	flour					1226:1230	tarwi flour	1220:1230	tarwi flour (50%)	1220:1236	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	7	58	mod	modified	1208:1215	arg3	%					1235:1235	50%	1233:1235	50%	1233:1235	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	10	59	theme	good	1680:1683	arg1	quality					1699:1705	good technological quality	1680:1705	good technological quality	1680:1705	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	2	60	theme	flours	510:515	arg1	%					499:499	50%	497:499	50% of these flours	497:515	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	2	60	theme	flours	510:515	arg1	flours					510:515	these flours	504:515	these flours	504:515	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	3	61	theme	high	560:563	arg1	fiber					574:578	high protein, fiber, and fat content	560:595	fiber	574:578	RESULTS Andean flours have high protein, fiber, and fat content, and display high water and oil absorption.					
35753031	6	62	theme	scanning	932:939	arg1	micrographs					941:951	Confocal laser scanning micrographs	917:951	Confocal laser scanning micrographs	917:951	Confocal laser scanning micrographs showed that batters with Andean flours presented a complex matrix with dispersed starch granules surrounded by proteins and fiber fragments.					
35753031	7	63	theme	lower	1141:1145	arg1	volume					1156:1161	a slightly lower specific volume	1130:1161	a slightly lower specific volume	1130:1161	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	4	64	from	reduction	704:712	arg1	viscosity					723:731	paste viscosity	717:731	paste viscosity	717:731	In premixes formulated with potato starch and Andean flours, a reduction in paste viscosity was observed due to starch dilution and lower water availability.					
35753031	1	65	theme	crops	129:133	arg1	flours					108:113	The flours	104:113	The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen),	104:206	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	6	66	theme	Confocal	917:924	arg1	micrographs					941:951	Confocal laser scanning micrographs	917:951	Confocal laser scanning micrographs	917:951	Confocal laser scanning micrographs showed that batters with Andean flours presented a complex matrix with dispersed starch granules surrounded by proteins and fiber fragments.					
35753031	0	67	theme	gluten-free	72:82	arg1	muffins					84:90	vegan gluten-free muffins	66:90	vegan gluten-free muffins	66:90	Andean crops: kañiwa and tarwi flours used for the development of vegan gluten-free muffins.					
35753031	2	68	dep	batters	439:445	arg1	starch-based					467:478	starch-based	467:478	starch-based	467:478	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	8	69	theme	reducing	1360:1367	arg1	content					1375:1381	their reducing sugar content	1354:1381	their reducing sugar content	1354:1381	The intrinsic color of these flours modified crumb color, and their reducing sugar content also favored Maillard reactions in the crust.					
35753031	4	70	theme	Andean	687:692	arg1	flours					694:699	Andean flours	687:699	Andean flours	687:699	In premixes formulated with potato starch and Andean flours, a reduction in paste viscosity was observed due to starch dilution and lower water availability.					
35753031	9	71	from	products	1478:1485	arg1	desirable					1453:1461	desirable	1453:1461	desirable	1453:1461	These color changes are desirable in gluten-free products because such products are frequently pale due to their high starch content.					
35753031	6	72	theme	complex	1004:1010	arg1	matrix					1012:1017	a complex matrix	1002:1017	a complex matrix	1002:1017	Confocal laser scanning micrographs showed that batters with Andean flours presented a complex matrix with dispersed starch granules surrounded by proteins and fiber fragments.					
35753031	2	73	theme	techno-functional	349:365	arg1	properties					367:376	techno-functional properties	349:376	techno-functional properties	349:376	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	0	74	theme	kañiwa	14:19	arg1	flours					31:36	kañiwa and tarwi flours	14:36	Andean crops: kañiwa and tarwi flours used for the development of vegan gluten-free muffins.	0:91	Andean crops: kañiwa and tarwi flours used for the development of vegan gluten-free muffins.					
35753031	0	75	dep	crops	7:11	arg1	flours					31:36	kañiwa and tarwi flours	14:36	Andean crops: kañiwa and tarwi flours used for the development of vegan gluten-free muffins.	0:91	Andean crops: kañiwa and tarwi flours used for the development of vegan gluten-free muffins.					
35753031	6	76	theme	Andean	978:983	arg1	flours					985:990	Andean flours	978:990	Andean flours	978:990	Confocal laser scanning micrographs showed that batters with Andean flours presented a complex matrix with dispersed starch granules surrounded by proteins and fiber fragments.					
35753031	2	77	theme	flours	398:403	arg1	composition					333:343	proximal composition	324:343	proximal composition	324:343	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	2	77	theme	flours	398:403	arg1	properties					367:376	techno-functional properties	349:376	techno-functional properties	349:376	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	3	78	theme	fat	585:587	arg1	content					589:595	high protein, fiber, and fat content	560:595	content	589:595	RESULTS Andean flours have high protein, fiber, and fat content, and display high water and oil absorption.					
35753031	0	79	theme	tarwi	25:29	arg1	flours					31:36	kañiwa and tarwi flours	14:36	Andean crops: kañiwa and tarwi flours used for the development of vegan gluten-free muffins.	0:91	Andean crops: kañiwa and tarwi flours used for the development of vegan gluten-free muffins.					
35753031	10	80	theme	quality	1699:1705	arg1	vegan					1650:1654	vegan	1650:1654	vegan	1650:1654	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	10	80	theme	quality	1699:1705	arg1	muffins					1669:1675	gluten-free muffins	1657:1675	gluten-free muffins of good technological quality and improved nutritional profile	1657:1738	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	7	81	contain	had	1126:1128	arg2	volume					1156:1161	a slightly lower specific volume	1130:1161	a slightly lower specific volume	1130:1161	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	7	81	contain	had	1126:1128	arg1	Muffins					1094:1100	Muffins	1094:1100	Muffins made with Andean flours	1094:1124	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	1	82	theme	vegan	266:270	arg1	formulations					291:302	vegan gluten-free muffin formulations	266:302	vegan gluten-free muffin formulations	266:302	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	2	83	theme	technological	414:426	arg1	quality					428:434	the technological quality	410:434	the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours	410:515	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	10	84	theme	underutilized	1763:1775	arg1	flours					1787:1792	these underutilized ancestral flours	1757:1792	these underutilized ancestral flours	1757:1792	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	1	85	theme	muffin	284:289	arg1	formulations					291:302	vegan gluten-free muffin formulations	266:302	vegan gluten-free muffin formulations	266:302	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	5	86	theme	higher	897:902	arg1	consistency					904:914	a higher consistency	895:914	a higher consistency	895:914	Depending on their ability to interact with water, the batters formulated with these flours had a higher consistency.					
35753031	7	87	theme	crumb	1185:1189	arg1	hardness					1191:1198	crumb hardness	1185:1198	crumb hardness	1185:1198	Muffins made with Andean flours had a slightly lower specific volume than the control, but crumb hardness was not modified by tarwi flour (50%) and a mixture of tarwi (25%) and kañiwa (25%) flours.					
35753031	10	88	theme	profile	1732:1738	arg1	vegan					1650:1654	vegan	1650:1654	vegan	1650:1654	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	10	88	theme	profile	1732:1738	arg1	muffins					1669:1675	gluten-free muffins	1657:1675	gluten-free muffins of good technological quality and improved nutritional profile	1657:1738	CONCLUSIONS This study showed that tarwi and kañiwa flours are suitable for developing vegan, gluten-free muffins of good technological quality and improved nutritional profile, adding value to these underutilized ancestral flours.					
35753031	2	89	dep	composition	333:343	arg1	the					320:322	the	320:322	the	320:322	In this study, the proximal composition and techno-functional properties of tarwi and kañiwa flours, and the technological quality of batters and muffins (potato starch-based) formulated with 50% of these flours were evaluated.					
35753031	1	90	theme	pallidicaule	186:197	arg1	Aellen					199:204	Chenopodium pallidicaule Aellen	174:204	Chenopodium pallidicaule Aellen	174:204	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	1	90	theme	pallidicaule	186:197	arg1	kañiwa					166:171	kañiwa	166:171	kañiwa (Chenopodium pallidicaule Aellen)	166:205	BACKGROUND The flours of two Andean crops, tarwi (Lupinus mutabilis) and kañiwa (Chenopodium pallidicaule Aellen), present an excellent nutritional profile for inclusion in vegan gluten-free muffin formulations.					
35753031	9	91	theme	gluten-free	1466:1476	arg1	products					1478:1485	gluten-free products	1466:1485	gluten-free products	1466:1485	These color changes are desirable in gluten-free products because such products are frequently pale due to their high starch content.					
35753031	6	92	theme	starch	1034:1039	arg1	granules					1041:1048	dispersed starch granules	1024:1048	dispersed starch granules surrounded by proteins and fiber fragments	1024:1091	Confocal laser scanning micrographs showed that batters with Andean flours presented a complex matrix with dispersed starch granules surrounded by proteins and fiber fragments.					
35753031	3	93	theme	oil	625:627	arg1	absorption					629:638	oil absorption	625:638	oil absorption	625:638	RESULTS Andean flours have high protein, fiber, and fat content, and display high water and oil absorption.					
35753031	4	94	theme	paste	717:721	arg1	viscosity					723:731	paste viscosity	717:731	paste viscosity	717:731	In premixes formulated with potato starch and Andean flours, a reduction in paste viscosity was observed due to starch dilution and lower water availability.					
36880274	3	0	theme	polymeric	320:328	arg1	film					354:357	a polymeric antibacterial composite film	318:357	a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate	318:429	Herein, we developed a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate.					
36880274	6	1	from	bacteria	940:947	arg1	wounds					957:962	real wounds	952:962	real wounds	952:962	In vivo animal tests showed that YSO-Pr/PVA/SA is effective and safe for inhibiting bacteria in real wounds.					
36880274	3	2	theme	antibacterial	330:342	arg1	film					354:357	a polymeric antibacterial composite film	318:357	a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate	318:429	Herein, we developed a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate.					
36880274	5	3	dep	bacteria	823:830	arg1	coli					790:793	Escherichia coli	778:793	Escherichia coli	778:793	The YSO-Pr/PVA/SA showed upconversion luminescence in photoluminescence spectrometry tests, and the emitted UVC inhibited Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in antibacterial tests.					
36880274	5	3	dep	bacteria	823:830	arg1	aeruginosa					811:820	Pseudomonas aeruginosa	799:820	Pseudomonas aeruginosa	799:820	The YSO-Pr/PVA/SA showed upconversion luminescence in photoluminescence spectrometry tests, and the emitted UVC inhibited Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in antibacterial tests.					
36880274	5	4	from	Gram-positive	721:733	arg1	tests					849:853	antibacterial tests	835:853	antibacterial tests	835:853	The YSO-Pr/PVA/SA showed upconversion luminescence in photoluminescence spectrometry tests, and the emitted UVC inhibited Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in antibacterial tests.					
36880274	5	5	theme	photoluminescence	653:669	arg1	tests					684:688	photoluminescence spectrometry tests	653:688	photoluminescence spectrometry tests	653:688	The YSO-Pr/PVA/SA showed upconversion luminescence in photoluminescence spectrometry tests, and the emitted UVC inhibited Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in antibacterial tests.					
36880274	7	6	theme	cytotoxicity	978:989	arg1	test					991:994	The in vitro cytotoxicity test	965:994	The in vitro cytotoxicity test	965:994	The in vitro cytotoxicity test further confirmed the good biocompatibility of the antibacterial film.					
36880274	5	7	theme	spectrometry	671:682	arg1	tests					684:688	photoluminescence spectrometry tests	653:688	photoluminescence spectrometry tests	653:688	The YSO-Pr/PVA/SA showed upconversion luminescence in photoluminescence spectrometry tests, and the emitted UVC inhibited Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in antibacterial tests.					
36880274	8	8	theme	sufficient	1104:1113	arg1	strength					1123:1130	sufficient tensile strength	1104:1130	sufficient tensile strength	1104:1130	In addition, YSO-Pr/PVA/SA exhibited sufficient tensile strength.					
36880274	4	9	theme	yttrium	465:471	arg1	Y2SiO5					488:493	Y2SiO5	488:493	Y2SiO5	488:493	The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria.					
36880274	4	9	theme	yttrium	465:471	arg1	orthosilicate					473:485	praseodymium-doped yttrium orthosilicate	446:485	praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC)	446:579	The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria.					
36880274	7	10	theme	in	969:970	arg1	test					991:994	The in vitro cytotoxicity test	965:994	The in vitro cytotoxicity test	965:994	The in vitro cytotoxicity test further confirmed the good biocompatibility of the antibacterial film.					
36880274	1	11	theme	Bacterial	78:86	arg1	infections					88:97	Bacterial infections	78:97	Bacterial infections caused by the growth and reproduction of pathogenic bacteria on wounds	78:168	Bacterial infections caused by the growth and reproduction of pathogenic bacteria on wounds are one of the main reasons that hinder wound healing.					
36880274	3	12	theme	polyvinyl	365:373	arg1	substrate					421:429	the substrate	417:429	the substrate	417:429	Herein, we developed a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate.					
36880274	3	12	theme	polyvinyl	365:373	arg1	PVA					384:386	PVA	384:386	PVA	384:386	Herein, we developed a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate.					
36880274	3	12	theme	polyvinyl	365:373	arg1	alcohol					375:381	polyvinyl alcohol	365:381	polyvinyl alcohol (PVA)	365:387	Herein, we developed a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate.					
36880274	3	12	theme	polyvinyl	365:373	arg1	alginate					400:407	sodium alginate	393:407	sodium alginate (SA)	393:412	Herein, we developed a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate.					
36880274	6	13	theme	animal	864:869	arg1	tests					871:875	In vivo animal tests	856:875	In vivo animal tests	856:875	In vivo animal tests showed that YSO-Pr/PVA/SA is effective and safe for inhibiting bacteria in real wounds.					
36880274	2	14	theme	bacterial	275:283	arg1	infections					285:294	bacterial infections	275:294	bacterial infections	275:294	Antibacterial wound dressings protect wounds from bacterial infections.					
36880274	6	15	theme	real	952:955	arg1	wounds					957:962	real wounds	952:962	real wounds	952:962	In vivo animal tests showed that YSO-Pr/PVA/SA is effective and safe for inhibiting bacteria in real wounds.					
36880274	6	16	dep	In	856:857	arg1	vivo					859:862	vivo	859:862	vivo	859:862	In vivo animal tests showed that YSO-Pr/PVA/SA is effective and safe for inhibiting bacteria in real wounds.					
36880274	3	17	theme	composite	344:352	arg1	film					354:357	a polymeric antibacterial composite film	318:357	a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate	318:429	Herein, we developed a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate.					
36880274	3	18	theme	sodium	393:398	arg1	substrate					421:429	the substrate	417:429	the substrate	417:429	Herein, we developed a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate.					
36880274	3	18	theme	sodium	393:398	arg1	alcohol					375:381	polyvinyl alcohol	365:381	polyvinyl alcohol (PVA)	365:387	Herein, we developed a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate.					
36880274	3	18	theme	sodium	393:398	arg1	SA					410:411	SA	410:411	SA	410:411	Herein, we developed a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate.					
36880274	3	18	theme	sodium	393:398	arg1	alginate					400:407	sodium alginate	393:407	sodium alginate (SA)	393:412	Herein, we developed a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate.					
36880274	4	19	dep	Y2SiO5	488:493	arg1	Pr3+					496:499	Pr3+	496:499	Pr3+	496:499	The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria.					
36880274	4	19	dep	Y2SiO5	488:493	arg1	YSO-Pr					502:507	YSO-Pr	502:507	YSO-Pr	502:507	The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria.					
36880274	5	20	theme	upconversion	624:635	arg1	luminescence					637:648	upconversion luminescence	624:648	upconversion luminescence	624:648	The YSO-Pr/PVA/SA showed upconversion luminescence in photoluminescence spectrometry tests, and the emitted UVC inhibited Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in antibacterial tests.					
36880274	1	21	theme	main	185:188	arg1	reasons					190:196	the main reasons	181:196	the main reasons that hinder wound healing	181:222	Bacterial infections caused by the growth and reproduction of pathogenic bacteria on wounds are one of the main reasons that hinder wound healing.					
36880274	5	22	theme	emitted	699:705	arg1	UVC					707:709	the emitted UVC	695:709	the emitted UVC	695:709	The YSO-Pr/PVA/SA showed upconversion luminescence in photoluminescence spectrometry tests, and the emitted UVC inhibited Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in antibacterial tests.					
36880274	1	23	from	reproduction	124:135	arg1	wounds					163:168	wounds	163:168	wounds	163:168	Bacterial infections caused by the growth and reproduction of pathogenic bacteria on wounds are one of the main reasons that hinder wound healing.					
36880274	1	24	theme	reasons	190:196	arg1	one					174:176	one	174:176	one	174:176	Bacterial infections caused by the growth and reproduction of pathogenic bacteria on wounds are one of the main reasons that hinder wound healing.					
36880274	1	24	theme	reasons	190:196	arg1	reasons					190:196	the main reasons	181:196	the main reasons that hinder wound healing	181:222	Bacterial infections caused by the growth and reproduction of pathogenic bacteria on wounds are one of the main reasons that hinder wound healing.					
36880274	0	25	theme	polymer	25:31	arg1	films					33:37	polymer films	25:37	polymer films for prevention of microbial infection	25:75	Visible-UVC upconversion polymer films for prevention of microbial infection.					
36880274	4	26	theme	ultraviolet	557:567	arg1	UVC					576:578	UVC	576:578	UVC	576:578	The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria.					
36880274	4	26	theme	ultraviolet	557:567	arg1	light					569:573	short-wavelength ultraviolet light	540:573	short-wavelength ultraviolet light (UVC)	540:579	The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria.					
36880274	7	27	theme	good	1018:1021	arg1	biocompatibility					1023:1038	the good biocompatibility	1014:1038	the good biocompatibility of the antibacterial film	1014:1064	The in vitro cytotoxicity test further confirmed the good biocompatibility of the antibacterial film.					
36880274	4	28	theme	short-wavelength	540:555	arg1	UVC					576:578	UVC	576:578	UVC	576:578	The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria.					
36880274	4	28	theme	short-wavelength	540:555	arg1	light					569:573	short-wavelength ultraviolet light	540:573	short-wavelength ultraviolet light (UVC)	540:579	The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria.					
36880274	2	29	theme	wound	239:243	arg1	dressings					245:253	Antibacterial wound dressings	225:253	Antibacterial wound dressings	225:253	Antibacterial wound dressings protect wounds from bacterial infections.					
36880274	4	30	theme	praseodymium-doped	446:463	arg1	Y2SiO5					488:493	Y2SiO5	488:493	Y2SiO5	488:493	The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria.					
36880274	4	30	theme	praseodymium-doped	446:463	arg1	orthosilicate					473:485	praseodymium-doped yttrium orthosilicate	446:485	praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC)	446:579	The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria.					
36880274	2	31	theme	Antibacterial	225:237	arg1	dressings					245:253	Antibacterial wound dressings	225:253	Antibacterial wound dressings	225:253	Antibacterial wound dressings protect wounds from bacterial infections.					
36880274	1	32	theme	wound	210:214	arg1	healing					216:222	wound healing	210:222	wound healing	210:222	Bacterial infections caused by the growth and reproduction of pathogenic bacteria on wounds are one of the main reasons that hinder wound healing.					
36880274	0	33	theme	microbial	57:65	arg1	infection					67:75	microbial infection	57:75	microbial infection	57:75	Visible-UVC upconversion polymer films for prevention of microbial infection.					
36880274	5	34	theme	Gram-negative	763:775	arg1	bacteria					823:830	Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria	721:830	bacteria	823:830	The YSO-Pr/PVA/SA showed upconversion luminescence in photoluminescence spectrometry tests, and the emitted UVC inhibited Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in antibacterial tests.					
36880274	4	35	theme	visible	521:527	arg1	light					529:533	visible light	521:533	visible light	521:533	The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria.					
36880274	8	36	theme	tensile	1115:1121	arg1	strength					1123:1130	sufficient tensile strength	1104:1130	sufficient tensile strength	1104:1130	In addition, YSO-Pr/PVA/SA exhibited sufficient tensile strength.					
36880274	5	37	from	tests	849:853	arg1	Gram-positive					721:733	Gram-positive	721:733	Gram-positive	721:733	The YSO-Pr/PVA/SA showed upconversion luminescence in photoluminescence spectrometry tests, and the emitted UVC inhibited Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in antibacterial tests.					
36880274	7	38	theme	antibacterial	1047:1059	arg1	film					1061:1064	the antibacterial film	1043:1064	the antibacterial film	1043:1064	The in vitro cytotoxicity test further confirmed the good biocompatibility of the antibacterial film.					
36880274	6	39	theme	In	856:857	arg1	tests					871:875	In vivo animal tests	856:875	In vivo animal tests	856:875	In vivo animal tests showed that YSO-Pr/PVA/SA is effective and safe for inhibiting bacteria in real wounds.					
36880274	9	40	theme	medical	1217:1223	arg1	dressings					1225:1233	medical dressings	1217:1233	medical dressings	1217:1233	Overall, this study demonstrates the potential of upconversion materials for use in medical dressings.					
36880274	1	41	from	growth	113:118	arg1	wounds					163:168	wounds	163:168	wounds	163:168	Bacterial infections caused by the growth and reproduction of pathogenic bacteria on wounds are one of the main reasons that hinder wound healing.					
36880274	7	42	theme	film	1061:1064	arg1	biocompatibility					1023:1038	the good biocompatibility	1014:1038	the good biocompatibility of the antibacterial film	1014:1064	The in vitro cytotoxicity test further confirmed the good biocompatibility of the antibacterial film.					
36880274	9	43	theme	upconversion	1183:1194	arg1	materials					1196:1204	upconversion materials	1183:1204	upconversion materials	1183:1204	Overall, this study demonstrates the potential of upconversion materials for use in medical dressings.					
36880274	1	44	theme	pathogenic	140:149	arg1	bacteria					151:158	pathogenic bacteria	140:158	pathogenic bacteria	140:158	Bacterial infections caused by the growth and reproduction of pathogenic bacteria on wounds are one of the main reasons that hinder wound healing.					
36880274	9	45	from	use	1210:1212	arg1	dressings					1225:1233	medical dressings	1217:1233	medical dressings	1217:1233	Overall, this study demonstrates the potential of upconversion materials for use in medical dressings.					
36880274	4	46	used	used	441:444	arg2	film					436:439	The film	432:439	The film	432:439	The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria.					
36880274	9	47	theme	materials	1196:1204	arg1	potential					1170:1178	the potential	1166:1178	the potential of upconversion materials for use in medical dressings	1166:1233	Overall, this study demonstrates the potential of upconversion materials for use in medical dressings.					
36880274	1	48	theme	bacteria	151:158	arg1	reproduction					124:135	reproduction	124:135	reproduction	124:135	Bacterial infections caused by the growth and reproduction of pathogenic bacteria on wounds are one of the main reasons that hinder wound healing.					
36880274	1	48	theme	bacteria	151:158	arg1	growth					113:118	growth	113:118	growth	113:118	Bacterial infections caused by the growth and reproduction of pathogenic bacteria on wounds are one of the main reasons that hinder wound healing.					
36880274	7	49	dep	in	969:970	arg1	vitro					972:976	vitro	972:976	vitro	972:976	The in vitro cytotoxicity test further confirmed the good biocompatibility of the antibacterial film.					
36880274	0	50	theme	infection	67:75	arg1	prevention					43:52	prevention	43:52	prevention of microbial infection	43:75	Visible-UVC upconversion polymer films for prevention of microbial infection.					
36880274	5	51	theme	antibacterial	835:847	arg1	tests					849:853	antibacterial tests	835:853	antibacterial tests	835:853	The YSO-Pr/PVA/SA showed upconversion luminescence in photoluminescence spectrometry tests, and the emitted UVC inhibited Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in antibacterial tests.					
36880274	5	52	dep	Gram-positive	721:733	arg1	aureus					751:756	Staphylococcus aureus	736:756	Staphylococcus aureus	736:756	The YSO-Pr/PVA/SA showed upconversion luminescence in photoluminescence spectrometry tests, and the emitted UVC inhibited Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in antibacterial tests.					
36674906	6	0	theme	rGO-CTS	966:972	arg1	cryogel					974:980	the rGO-CTS cryogel	962:980	the rGO-CTS cryogel	962:980	In mouse liver trauma models, the rGO-CTS cryogel showed good blood clotting and hemostatic capabilities.					
36674906	1	1	theme	fast	174:177	arg1	recovery					179:186	fast recovery	174:186	fast recovery	174:186	In this study, an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds was prepared using a one-step method.					
36674906	7	2	theme	non-compressible	1140:1155	arg1	wounds					1157:1162	non-compressible wounds	1140:1162	non-compressible wounds	1140:1162	Therefore, this composite cryogel has great potential as a new hemostatic material for application to non-compressible wounds.					
36674906	2	3	theme	mixing	372:377	arg1	solution					379:386	a frozen mixing solution	363:386	a frozen mixing solution of chitosan	363:398	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	7	4	contain	has	1072:1074	arg2	potential					1082:1090	great potential	1076:1090	great potential	1076:1090	Therefore, this composite cryogel has great potential as a new hemostatic material for application to non-compressible wounds.					
36674906	7	4	contain	has	1072:1074	arg2	material					1112:1119	a new hemostatic material	1095:1119	a new hemostatic material for application to non-compressible wounds	1095:1162	Therefore, this composite cryogel has great potential as a new hemostatic material for application to non-compressible wounds.					
36674906	7	4	contain	has	1072:1074	arg1	cryogel					1064:1070	this composite cryogel	1049:1070	this composite cryogel	1049:1070	Therefore, this composite cryogel has great potential as a new hemostatic material for application to non-compressible wounds.					
36674906	4	5	theme	excellent	769:777	arg1	properties					806:815	excellent water-induced shape-memory properties	769:815	excellent water-induced shape-memory properties	769:815	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	2	6	theme	one-step	342:349	arg1	acid					408:411	citric acid	401:411	citric acid	401:411	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	2	6	theme	one-step	342:349	arg1	method					351:356	a one-step method	340:356	a one-step method with a frozen mixing solution of chitosan	340:398	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	2	6	theme	one-step	342:349	arg1	oxide					437:441	graphene oxide	428:441	graphene oxide	428:441	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	2	6	theme	one-step	342:349	arg1	dopamine					414:421	dopamine	414:421	dopamine	414:421	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	3	7	theme	chitosan	556:563	arg1	cross-linking					539:551	the double cross-linking	528:551	the double cross-linking of chitosan	528:563	The alkaline solution not only promoted the double cross-linking of chitosan but also induced dopamine to form polydopamine-reducing graphene oxide.					
36674906	2	8	theme	rGO-CTS	317:323	arg1	cryogel					326:332	a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel	275:332	a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions	275:485	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	4	9	theme	network	738:744	arg1	structure					746:754	a uniform porous network structure	721:754	a uniform porous network structure	721:754	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	3	10	theme	alkaline	492:499	arg1	solution					501:508	The alkaline solution	488:508	The alkaline solution	488:508	The alkaline solution not only promoted the double cross-linking of chitosan but also induced dopamine to form polydopamine-reducing graphene oxide.					
36674906	5	11	theme	antibacterial	886:898	arg1	activity					900:907	antibacterial activity	886:907	antibacterial activity	886:907	Moreover, the rGO-CTS cryogel exhibited good mechanical properties, antibacterial activity, and biocompatibility.					
36674906	3	12	theme	double	532:537	arg1	cross-linking					539:551	the double cross-linking	528:551	the double cross-linking of chitosan	528:563	The alkaline solution not only promoted the double cross-linking of chitosan but also induced dopamine to form polydopamine-reducing graphene oxide.					
36674906	6	13	theme	liver	941:945	arg1	models					954:959	mouse liver trauma models	935:959	mouse liver trauma models	935:959	In mouse liver trauma models, the rGO-CTS cryogel showed good blood clotting and hemostatic capabilities.					
36674906	2	14	theme	graphene/chitosan	298:314	arg1	cryogel					326:332	a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel	275:332	a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions	275:485	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	1	15	theme	non-compressible	193:208	arg1	wounds					210:215	non-compressible wounds	193:215	non-compressible wounds	193:215	In this study, an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds was prepared using a one-step method.					
36674906	0	16	theme	Graphene/Chitosan	21:37	arg1	Cryogels					39:46	Shape-Memory-Reduced Graphene/Chitosan Cryogels	0:46	Shape-Memory-Reduced Graphene/Chitosan Cryogels for Non-Compressible Wounds.	0:75	Shape-Memory-Reduced Graphene/Chitosan Cryogels for Non-Compressible Wounds.					
36674906	6	17	theme	mouse	935:939	arg1	models					954:959	mouse liver trauma models	935:959	mouse liver trauma models	935:959	In mouse liver trauma models, the rGO-CTS cryogel showed good blood clotting and hemostatic capabilities.					
36674906	1	18	with	cryogel	135:141	arg1	absorption					159:168	high blood absorption	148:168	high blood absorption	148:168	In this study, an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds was prepared using a one-step method.					
36674906	1	18	with	cryogel	135:141	arg1	recovery					179:186	fast recovery	174:186	fast recovery	174:186	In this study, an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds was prepared using a one-step method.					
36674906	1	19	theme	antibacterial	95:107	arg1	cryogel					135:141	an antibacterial and shape-memory chitosan cryogel	92:141	an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds	92:215	In this study, an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds was prepared using a one-step method.					
36674906	6	20	theme	trauma	947:952	arg1	models					954:959	mouse liver trauma models	935:959	mouse liver trauma models	935:959	In mouse liver trauma models, the rGO-CTS cryogel showed good blood clotting and hemostatic capabilities.					
36674906	2	21	theme	citric	401:406	arg1	acid					408:411	citric acid	401:411	citric acid	401:411	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	2	21	theme	citric	401:406	arg1	method					351:356	a one-step method	340:356	a one-step method with a frozen mixing solution of chitosan	340:398	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	0	22	theme	Shape-Memory-Reduced	0:19	arg1	Cryogels					39:46	Shape-Memory-Reduced Graphene/Chitosan Cryogels	0:46	Shape-Memory-Reduced Graphene/Chitosan Cryogels for Non-Compressible Wounds.	0:75	Shape-Memory-Reduced Graphene/Chitosan Cryogels for Non-Compressible Wounds.					
36674906	5	23	theme	mechanical	863:872	arg1	properties					874:883	good mechanical properties	858:883	good mechanical properties	858:883	Moreover, the rGO-CTS cryogel exhibited good mechanical properties, antibacterial activity, and biocompatibility.					
36674906	5	24	theme	good	858:861	arg1	properties					874:883	good mechanical properties	858:883	good mechanical properties	858:883	Moreover, the rGO-CTS cryogel exhibited good mechanical properties, antibacterial activity, and biocompatibility.					
36674906	1	25	theme	shape-memory	113:124	arg1	cryogel					135:141	an antibacterial and shape-memory chitosan cryogel	92:141	an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds	92:215	In this study, an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds was prepared using a one-step method.					
36674906	2	26	theme	graphene	428:435	arg1	oxide					437:441	graphene oxide	428:441	graphene oxide	428:441	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	2	26	theme	graphene	428:435	arg1	method					351:356	a one-step method	340:356	a one-step method with a frozen mixing solution of chitosan	340:398	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	6	27	theme	hemostatic	1013:1022	arg1	capabilities					1024:1035	hemostatic capabilities	1013:1035	hemostatic capabilities	1013:1035	In mouse liver trauma models, the rGO-CTS cryogel showed good blood clotting and hemostatic capabilities.					
36674906	1	28	theme	chitosan	126:133	arg1	cryogel					135:141	an antibacterial and shape-memory chitosan cryogel	92:141	an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds	92:215	In this study, an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds was prepared using a one-step method.					
36674906	4	29	theme	microscope	655:664	arg1	images					672:677	Scanning electron microscope (SEM) images	637:677	Scanning electron microscope (SEM) images	637:677	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	2	30	with	method	351:356	arg1	solution					379:386	a frozen mixing solution	363:386	a frozen mixing solution of chitosan	363:398	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	3	31	theme	polydopamine-reducing	599:619	arg1	oxide					630:634	polydopamine-reducing graphene oxide	599:634	polydopamine-reducing graphene oxide	599:634	The alkaline solution not only promoted the double cross-linking of chitosan but also induced dopamine to form polydopamine-reducing graphene oxide.					
36674906	2	32	theme	alkaline	468:475	arg1	solutions					477:485	alkaline solutions	468:485	alkaline solutions	468:485	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	0	33	theme	Non-Compressible	52:67	arg1	Wounds					69:74	Non-Compressible Wounds	52:74	Non-Compressible Wounds	52:74	Shape-Memory-Reduced Graphene/Chitosan Cryogels for Non-Compressible Wounds.					
36674906	4	34	theme	shape-memory	793:804	arg1	properties					806:815	excellent water-induced shape-memory properties	769:815	excellent water-induced shape-memory properties	769:815	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	3	35	theme	graphene	621:628	arg1	oxide					630:634	polydopamine-reducing graphene oxide	599:634	polydopamine-reducing graphene oxide	599:634	The alkaline solution not only promoted the double cross-linking of chitosan but also induced dopamine to form polydopamine-reducing graphene oxide.					
36674906	7	36	theme	great	1076:1080	arg1	material					1112:1119	a new hemostatic material	1095:1119	a new hemostatic material for application to non-compressible wounds	1095:1162	Therefore, this composite cryogel has great potential as a new hemostatic material for application to non-compressible wounds.					
36674906	7	36	theme	great	1076:1080	arg1	potential					1082:1090	great potential	1076:1090	great potential	1076:1090	Therefore, this composite cryogel has great potential as a new hemostatic material for application to non-compressible wounds.					
36674906	1	37	theme	one-step	238:245	arg1	method					247:252	a one-step method	236:252	a one-step method	236:252	In this study, an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds was prepared using a one-step method.					
36674906	5	38	theme	rGO-CTS	832:838	arg1	cryogel					840:846	the rGO-CTS cryogel	828:846	the rGO-CTS cryogel	828:846	Moreover, the rGO-CTS cryogel exhibited good mechanical properties, antibacterial activity, and biocompatibility.					
36674906	6	39	theme	blood	994:998	arg1	clotting					1000:1007	good blood clotting	989:1007	good blood clotting	989:1007	In mouse liver trauma models, the rGO-CTS cryogel showed good blood clotting and hemostatic capabilities.					
36674906	7	40	theme	new	1097:1099	arg1	material					1112:1119	a new hemostatic material	1095:1119	a new hemostatic material for application to non-compressible wounds	1095:1162	Therefore, this composite cryogel has great potential as a new hemostatic material for application to non-compressible wounds.					
36674906	7	40	theme	new	1097:1099	arg1	potential					1082:1090	great potential	1076:1090	great potential	1076:1090	Therefore, this composite cryogel has great potential as a new hemostatic material for application to non-compressible wounds.					
36674906	2	41	theme	frozen	365:370	arg1	solution					379:386	a frozen mixing solution	363:386	a frozen mixing solution of chitosan	363:398	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	6	42	theme	good	989:992	arg1	clotting					1000:1007	good blood clotting	989:1007	good blood clotting	989:1007	In mouse liver trauma models, the rGO-CTS cryogel showed good blood clotting and hemostatic capabilities.					
36674906	7	43	theme	hemostatic	1101:1110	arg1	material					1112:1119	a new hemostatic material	1095:1119	a new hemostatic material for application to non-compressible wounds	1095:1162	Therefore, this composite cryogel has great potential as a new hemostatic material for application to non-compressible wounds.					
36674906	7	43	theme	hemostatic	1101:1110	arg1	potential					1082:1090	great potential	1076:1090	great potential	1076:1090	Therefore, this composite cryogel has great potential as a new hemostatic material for application to non-compressible wounds.					
36674906	4	44	theme	uniform	723:729	arg1	structure					746:754	a uniform porous network structure	721:754	a uniform porous network structure	721:754	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	4	45	theme	porous	731:736	arg1	structure					746:754	a uniform porous network structure	721:754	a uniform porous network structure	721:754	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	2	46	theme	shape-memory-reduced	277:296	arg1	cryogel					326:332	a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel	275:332	a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions	275:485	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	1	47	theme	high	148:151	arg1	absorption					159:168	high blood absorption	148:168	high blood absorption	148:168	In this study, an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds was prepared using a one-step method.					
36674906	4	48	theme	water-induced	779:791	arg1	properties					806:815	excellent water-induced shape-memory properties	769:815	excellent water-induced shape-memory properties	769:815	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	1	49	from	wounds	210:215	arg1	absorption					159:168	high blood absorption	148:168	high blood absorption	148:168	In this study, an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds was prepared using a one-step method.					
36674906	1	49	from	wounds	210:215	arg1	recovery					179:186	fast recovery	174:186	fast recovery	174:186	In this study, an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds was prepared using a one-step method.					
36674906	1	50	theme	blood	153:157	arg1	absorption					159:168	high blood absorption	148:168	high blood absorption	148:168	In this study, an antibacterial and shape-memory chitosan cryogel with high blood absorption and fast recovery from non-compressible wounds was prepared using a one-step method.					
36674906	7	51	theme	composite	1054:1062	arg1	cryogel					1064:1070	this composite cryogel	1049:1070	this composite cryogel	1049:1070	Therefore, this composite cryogel has great potential as a new hemostatic material for application to non-compressible wounds.					
36674906	2	52	theme	chitosan	391:398	arg1	solution					379:386	a frozen mixing solution	363:386	a frozen mixing solution of chitosan	363:398	Herein, we prepared a shape-memory-reduced graphene/chitosan (rGO-CTS) cryogel using a one-step method with a frozen mixing solution of chitosan, citric acid, dopamine, and graphene oxide, before treating it with alkaline solutions.					
36674906	4	53	theme	electron	646:653	arg1	SEM					667:669	SEM	667:669	SEM	667:669	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	4	53	theme	electron	646:653	arg1	microscope					655:664	Scanning electron microscope	637:664	Scanning electron microscope (SEM) images	637:677	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	4	54	contain	possessed	711:719	arg2	structure					746:754	a uniform porous network structure	721:754	a uniform porous network structure	721:754	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	4	54	contain	possessed	711:719	arg1	cryogel					703:709	the rGO-CTS cryogel	691:709	the rGO-CTS cryogel	691:709	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	4	55	theme	rGO-CTS	695:701	arg1	cryogel					703:709	the rGO-CTS cryogel	691:709	the rGO-CTS cryogel	691:709	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	4	56	theme	Scanning	637:644	arg1	SEM					667:669	SEM	667:669	SEM	667:669	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
36674906	4	56	theme	Scanning	637:644	arg1	microscope					655:664	Scanning electron microscope	637:664	Scanning electron microscope (SEM) images	637:677	Scanning electron microscope (SEM) images showed that the rGO-CTS cryogel possessed a uniform porous network structure, attributing excellent water-induced shape-memory properties.					
35412807	8	0	theme	biodegradable	1260:1272	arg1	scaffold					1288:1295	a conjugatable and biodegradable microparticle scaffold	1241:1295	a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest	1241:1371	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	8	0	theme	biodegradable	1260:1272	arg1	microparticles					1222:1235	chemically modified bacterial PGN microparticles	1188:1235	chemically modified bacterial PGN microparticles	1188:1235	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	7	1	theme	antibody	1074:1081	arg1	titers					1083:1088	neutralizing antibody titers	1061:1088	neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD	1061:1143	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	4	2	theme	microparticles	535:548	arg1	integrity					498:506	integrity	498:506	integrity	498:506	We first evaluated the purification, integrity, and immunogenicity of PGN microparticles derived from a variety of bacterial species.					
35412807	4	2	theme	microparticles	535:548	arg1	immunogenicity					513:526	immunogenicity	513:526	immunogenicity	513:526	We first evaluated the purification, integrity, and immunogenicity of PGN microparticles derived from a variety of bacterial species.					
35412807	4	2	theme	microparticles	535:548	arg1	purification					484:495	purification	484:495	purification	484:495	We first evaluated the purification, integrity, and immunogenicity of PGN microparticles derived from a variety of bacterial species.					
35412807	5	3	theme	microparticle	617:629	arg1	conditions					644:653	PGN microparticle modification conditions	613:653	PGN microparticle modification conditions	613:653	We then optimized PGN microparticle modification conditions; Staphylococcus aureus PGN microparticles containing azido-d-alanine yielded robust conjugation to immunogens.					
35412807	3	4	theme	PGN	349:351	arg1	microparticles					353:366	The PGN microparticles	345:366	The PGN microparticles	345:366	The PGN microparticles contain bio-orthogonal chemical handles allowing for site-specific attachment of immunogens.					
35412807	4	5	theme	PGN	531:533	arg1	microparticles					535:548	PGN microparticles	531:548	PGN microparticles derived from a variety of bacterial species	531:592	We first evaluated the purification, integrity, and immunogenicity of PGN microparticles derived from a variety of bacterial species.					
35412807	3	6	dep	chemical	391:398	arg1	handles					400:406	handles	400:406	handles	400:406	The PGN microparticles contain bio-orthogonal chemical handles allowing for site-specific attachment of immunogens.					
35412807	5	7	theme	modification	631:642	arg1	conditions					644:653	PGN microparticle modification conditions	613:653	PGN microparticle modification conditions	613:653	We then optimized PGN microparticle modification conditions; Staphylococcus aureus PGN microparticles containing azido-d-alanine yielded robust conjugation to immunogens.					
35412807	7	8	theme	PGN	955:957	arg1	microparticle					959:971	the S. aureus PGN microparticle	941:971	the S. aureus PGN microparticle	941:971	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	8	9	theme	microparticle	1274:1286	arg1	scaffold					1288:1295	a conjugatable and biodegradable microparticle scaffold	1241:1295	a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest	1241:1371	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	8	9	theme	microparticle	1274:1286	arg1	microparticles					1222:1235	chemically modified bacterial PGN microparticles	1188:1235	chemically modified bacterial PGN microparticles	1188:1235	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	5	10	theme	robust	732:737	arg1	conjugation					739:749	robust conjugation	732:749	robust conjugation to immunogens	732:763	We then optimized PGN microparticle modification conditions; Staphylococcus aureus PGN microparticles containing azido-d-alanine yielded robust conjugation to immunogens.					
35412807	6	11	theme	limpet	896:901	arg1	protein					879:885	the conventional carrier protein	854:885	the conventional carrier protein	854:885	We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH).					
35412807	6	11	theme	limpet	896:901	arg1	KLH					915:917	KLH	915:917	KLH	915:917	We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH).					
35412807	6	11	theme	limpet	896:901	arg1	hemocyanin					903:912	keyhole limpet hemocyanin	888:912	keyhole limpet hemocyanin (KLH)	888:918	We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH).					
35412807	5	12	theme	Staphylococcus	656:669	arg1	microparticles					682:695	Staphylococcus aureus PGN microparticles	656:695	Staphylococcus aureus PGN microparticles containing azido-d-alanine	656:722	We then optimized PGN microparticle modification conditions; Staphylococcus aureus PGN microparticles containing azido-d-alanine yielded robust conjugation to immunogens.					
35412807	6	13	theme	keyhole	888:894	arg1	protein					879:885	the conventional carrier protein	854:885	the conventional carrier protein	854:885	We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH).					
35412807	6	13	theme	keyhole	888:894	arg1	KLH					915:917	KLH	915:917	KLH	915:917	We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH).					
35412807	6	13	theme	keyhole	888:894	arg1	hemocyanin					903:912	keyhole limpet hemocyanin	888:912	keyhole limpet hemocyanin (KLH)	888:918	We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH).					
35412807	1	14	theme	Vaccine	75:81	arg1	scaffolds					83:91	Vaccine scaffolds	75:91	Vaccine scaffolds	75:91	Vaccine scaffolds and carrier proteins increase the immunogenicity of subunit vaccines.					
35412807	7	15	theme	S.	945:946	arg1	microparticle					959:971	the S. aureus PGN microparticle	941:971	the S. aureus PGN microparticle	941:971	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	8	16	theme	interest	1364:1371	arg1	antigen					1353:1359	an antigen	1350:1359	an antigen of interest	1350:1371	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	8	17	theme	capable	1297:1303	arg1	scaffold					1288:1295	a conjugatable and biodegradable microparticle scaffold	1241:1295	a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest	1241:1371	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	8	17	theme	capable	1297:1303	arg1	microparticles					1222:1235	chemically modified bacterial PGN microparticles	1188:1235	chemically modified bacterial PGN microparticles	1188:1235	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	7	18	theme	─this	1028:1032	arg1	vaccine					1044:1050	the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine	984:1050	the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD	984:1143	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	5	19	theme	PGN	678:680	arg1	microparticles					682:695	Staphylococcus aureus PGN microparticles	656:695	Staphylococcus aureus PGN microparticles containing azido-d-alanine	656:722	We then optimized PGN microparticle modification conditions; Staphylococcus aureus PGN microparticles containing azido-d-alanine yielded robust conjugation to immunogens.					
35412807	3	20	contain	contain	368:374	arg2	chemical					391:398	chemical	391:398	chemical	391:398	The PGN microparticles contain bio-orthogonal chemical handles allowing for site-specific attachment of immunogens.					
35412807	3	20	contain	contain	368:374	arg1	microparticles					353:366	The PGN microparticles	345:366	The PGN microparticles	345:366	The PGN microparticles contain bio-orthogonal chemical handles allowing for site-specific attachment of immunogens.					
35412807	4	21	attach	derived	550:556	arg2	microparticles					535:548	PGN microparticles	531:548	PGN microparticles derived from a variety of bacterial species	531:592	We first evaluated the purification, integrity, and immunogenicity of PGN microparticles derived from a variety of bacterial species.					
35412807	4	21	attach	derived	550:556	arg1	variety					565:571	a variety	563:571	a variety of bacterial species	563:592	We first evaluated the purification, integrity, and immunogenicity of PGN microparticles derived from a variety of bacterial species.					
35412807	4	21	attach	derived	550:556	arg1	species					586:592	bacterial species	576:592	bacterial species	576:592	We first evaluated the purification, integrity, and immunogenicity of PGN microparticles derived from a variety of bacterial species.					
35412807	4	22	theme	bacterial	576:584	arg1	species					586:592	bacterial species	576:592	bacterial species	576:592	We first evaluated the purification, integrity, and immunogenicity of PGN microparticles derived from a variety of bacterial species.					
35412807	6	23	theme	carrier	871:877	arg1	protein					879:885	the conventional carrier protein	854:885	the conventional carrier protein	854:885	We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH).					
35412807	6	23	theme	carrier	871:877	arg1	hemocyanin					903:912	keyhole limpet hemocyanin	888:912	keyhole limpet hemocyanin (KLH)	888:918	We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH).					
35412807	1	24	theme	carrier	97:103	arg1	proteins					105:112	carrier proteins	97:112	carrier proteins	97:112	Vaccine scaffolds and carrier proteins increase the immunogenicity of subunit vaccines.					
35412807	3	25	theme	immunogens	449:458	arg1	attachment					435:444	site-specific attachment	421:444	site-specific attachment of immunogens	421:458	The PGN microparticles contain bio-orthogonal chemical handles allowing for site-specific attachment of immunogens.					
35412807	8	26	theme	immune	1327:1332	arg1	response					1334:1341	a robust immune response	1318:1341	a robust immune response toward an antigen of interest	1318:1371	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	7	27	theme	domain	1016:1021	arg1	vaccine					1044:1050	the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine	984:1050	the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD	984:1143	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	5	28	theme	PGN	613:615	arg1	conditions					644:653	PGN microparticle modification conditions	613:653	PGN microparticle modification conditions	613:653	We then optimized PGN microparticle modification conditions; Staphylococcus aureus PGN microparticles containing azido-d-alanine yielded robust conjugation to immunogens.					
35412807	0	29	theme	Bacterial	20:28	arg1	Scaffold					65:72	a Vaccine Microparticle Scaffold	41:72	a Vaccine Microparticle Scaffold	41:72	Chemically Modified Bacterial Sacculi as a Vaccine Microparticle Scaffold.					
35412807	0	29	theme	Bacterial	20:28	arg1	Sacculi					30:36	Bacterial Sacculi	20:36	Bacterial Sacculi	20:36	Chemically Modified Bacterial Sacculi as a Vaccine Microparticle Scaffold.					
35412807	2	30	theme	bacterial	283:291	arg1	peptidoglycan					293:305	bacterial peptidoglycan	283:305	bacterial peptidoglycan (PGN)	283:311	Here, we developed, characterized, and demonstrated the efficacy of a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi.					
35412807	2	30	theme	bacterial	283:291	arg1	PGN					308:310	PGN	308:310	PGN	308:310	Here, we developed, characterized, and demonstrated the efficacy of a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi.					
35412807	7	31	theme	conjugate	1034:1042	arg1	vaccine					1044:1050	the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine	984:1050	the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD	984:1143	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	3	32	theme	site-specific	421:433	arg1	attachment					435:444	site-specific attachment	421:444	site-specific attachment of immunogens	421:458	The PGN microparticles contain bio-orthogonal chemical handles allowing for site-specific attachment of immunogens.					
35412807	4	33	theme	species	586:592	arg1	variety					565:571	a variety	563:571	a variety of bacterial species	563:592	We first evaluated the purification, integrity, and immunogenicity of PGN microparticles derived from a variety of bacterial species.					
35412807	4	33	theme	species	586:592	arg1	species					586:592	bacterial species	576:592	bacterial species	576:592	We first evaluated the purification, integrity, and immunogenicity of PGN microparticles derived from a variety of bacterial species.					
35412807	8	34	theme	modified	1199:1206	arg1	scaffold					1288:1295	a conjugatable and biodegradable microparticle scaffold	1241:1295	a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest	1241:1371	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	8	34	theme	modified	1199:1206	arg1	microparticles					1222:1235	chemically modified bacterial PGN microparticles	1188:1235	chemically modified bacterial PGN microparticles	1188:1235	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	2	35	theme	novel	233:237	arg1	scaffold					261:268	a novel microparticle vaccine scaffold	231:268	a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi	231:342	Here, we developed, characterized, and demonstrated the efficacy of a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi.					
35412807	8	36	theme	robust	1320:1325	arg1	response					1334:1341	a robust immune response	1318:1341	a robust immune response toward an antigen of interest	1318:1371	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	7	37	dep	S.	945:946	arg1	aureus					948:953	aureus	948:953	aureus	948:953	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	0	38	theme	Microparticle	51:63	arg1	Scaffold					65:72	a Vaccine Microparticle Scaffold	41:72	a Vaccine Microparticle Scaffold	41:72	Chemically Modified Bacterial Sacculi as a Vaccine Microparticle Scaffold.					
35412807	0	38	theme	Microparticle	51:63	arg1	Sacculi					30:36	Bacterial Sacculi	20:36	Bacterial Sacculi	20:36	Chemically Modified Bacterial Sacculi as a Vaccine Microparticle Scaffold.					
35412807	8	39	theme	PGN	1218:1220	arg1	scaffold					1288:1295	a conjugatable and biodegradable microparticle scaffold	1241:1295	a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest	1241:1371	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	8	39	theme	PGN	1218:1220	arg1	microparticles					1222:1235	chemically modified bacterial PGN microparticles	1188:1235	chemically modified bacterial PGN microparticles	1188:1235	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	0	40	theme	Vaccine	43:49	arg1	Scaffold					65:72	a Vaccine Microparticle Scaffold	41:72	a Vaccine Microparticle Scaffold	41:72	Chemically Modified Bacterial Sacculi as a Vaccine Microparticle Scaffold.					
35412807	0	40	theme	Vaccine	43:49	arg1	Sacculi					30:36	Bacterial Sacculi	20:36	Bacterial Sacculi	20:36	Chemically Modified Bacterial Sacculi as a Vaccine Microparticle Scaffold.					
35412807	8	41	theme	bacterial	1208:1216	arg1	scaffold					1288:1295	a conjugatable and biodegradable microparticle scaffold	1241:1295	a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest	1241:1371	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	8	41	theme	bacterial	1208:1216	arg1	microparticles					1222:1235	chemically modified bacterial PGN microparticles	1188:1235	chemically modified bacterial PGN microparticles	1188:1235	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	2	42	theme	entire	329:334	arg1	sacculi					336:342	an entire sacculi	326:342	an entire sacculi	326:342	Here, we developed, characterized, and demonstrated the efficacy of a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi.					
35412807	7	43	theme	SARS-CoV-2	988:997	arg1	RBD					1024:1026	RBD	1024:1026	RBD	1024:1026	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	7	43	theme	SARS-CoV-2	988:997	arg1	domain					1016:1021	the SARS-CoV-2 receptor-binding domain	984:1021	the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD	984:1143	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	6	44	theme	conventional	858:869	arg1	protein					879:885	the conventional carrier protein	854:885	the conventional carrier protein	854:885	We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH).					
35412807	6	44	theme	conventional	858:869	arg1	hemocyanin					903:912	keyhole limpet hemocyanin	888:912	keyhole limpet hemocyanin (KLH)	888:918	We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH).					
35412807	6	45	theme	comparable	822:831	arg1	immunostimulation					833:849	comparable immunostimulation	822:849	comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH)	822:918	We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH).					
35412807	7	46	theme	neutralizing	1061:1072	arg1	titers					1083:1088	neutralizing antibody titers	1061:1088	neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD	1061:1143	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	7	47	theme	KLH-conjugated	1126:1139	arg1	RBD					1141:1143	the KLH-conjugated RBD	1122:1143	the KLH-conjugated RBD	1122:1143	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	2	48	theme	scaffold	261:268	arg1	efficacy					219:226	the efficacy	215:226	the efficacy of a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi	215:342	Here, we developed, characterized, and demonstrated the efficacy of a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi.					
35412807	1	49	theme	subunit	145:151	arg1	vaccines					153:160	subunit vaccines	145:160	subunit vaccines	145:160	Vaccine scaffolds and carrier proteins increase the immunogenicity of subunit vaccines.					
35412807	8	50	theme	conjugatable	1243:1254	arg1	scaffold					1288:1295	a conjugatable and biodegradable microparticle scaffold	1241:1295	a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest	1241:1371	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	8	50	theme	conjugatable	1243:1254	arg1	microparticles					1222:1235	chemically modified bacterial PGN microparticles	1188:1235	chemically modified bacterial PGN microparticles	1188:1235	Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.					
35412807	2	51	theme	vaccine	253:259	arg1	scaffold					261:268	a novel microparticle vaccine scaffold	231:268	a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi	231:342	Here, we developed, characterized, and demonstrated the efficacy of a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi.					
35412807	7	52	theme	comparable	1090:1099	arg1	titers					1083:1088	neutralizing antibody titers	1061:1088	neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD	1061:1143	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	1	53	theme	vaccines	153:160	arg1	immunogenicity					127:140	the immunogenicity	123:140	the immunogenicity of subunit vaccines	123:160	Vaccine scaffolds and carrier proteins increase the immunogenicity of subunit vaccines.					
35412807	7	54	theme	receptor-binding	999:1014	arg1	RBD					1024:1026	RBD	1024:1026	RBD	1024:1026	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	7	54	theme	receptor-binding	999:1014	arg1	domain					1016:1021	the SARS-CoV-2 receptor-binding domain	984:1021	the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD	984:1143	We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)─this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD.					
35412807	5	55	dep	Staphylococcus	656:669	arg1	aureus					671:676	aureus	671:676	aureus	671:676	We then optimized PGN microparticle modification conditions; Staphylococcus aureus PGN microparticles containing azido-d-alanine yielded robust conjugation to immunogens.					
35412807	2	56	theme	microparticle	239:251	arg1	scaffold					261:268	a novel microparticle vaccine scaffold	231:268	a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi	231:342	Here, we developed, characterized, and demonstrated the efficacy of a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi.					
35412807	6	57	theme	vaccine	797:803	arg1	scaffold					805:812	this vaccine scaffold	792:812	this vaccine scaffold	792:812	We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH).					
35412807	5	58	contain	containing	697:706	arg2	azido-d-alanine					708:722	azido-d-alanine	708:722	azido-d-alanine	708:722	We then optimized PGN microparticle modification conditions; Staphylococcus aureus PGN microparticles containing azido-d-alanine yielded robust conjugation to immunogens.					
35412807	5	58	contain	containing	697:706	arg1	microparticles					682:695	Staphylococcus aureus PGN microparticles	656:695	Staphylococcus aureus PGN microparticles containing azido-d-alanine	656:722	We then optimized PGN microparticle modification conditions; Staphylococcus aureus PGN microparticles containing azido-d-alanine yielded robust conjugation to immunogens.					
35259440	6	0	theme	dry	973:975	arg1	powder					977:982	the anti-PD1-1-LL-PBS dry powder	951:982	the anti-PD1-1-LL-PBS dry powder	951:982	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	2	1	theme	dry	332:334	arg1	powders					336:342	aerosolizable dry powders	318:342	aerosolizable dry powders of monoclonal antibodies (mAbs)	318:374	Herein we report the preparation of aerosolizable dry powders of monoclonal antibodies (mAbs) by TFFD.					
35259440	15	2	with	powder	2270:2275	arg1	composition					2292:2302	a similar composition	2282:2302	a similar composition by TFFD	2282:2310	Finally, we showed that anti-TNF-α, another mAb, can also be converted to a dry powder with a similar composition by TFFD.					
35259440	1	3	theme	Thin-film	93:101	arg1	freeze-drying					103:115	Thin-film freeze-drying	93:115	Thin-film freeze-drying (TFFD)	93:122	Thin-film freeze-drying (TFFD) is a rapid freezing and then drying technique used to prepare inhalable dry powders from the liquid form for applications such as drug delivery to the lungs.					
35259440	1	3	theme	Thin-film	93:101	arg1	freezing					135:142	a rapid freezing and then drying technique	127:168	freezing	135:142	Thin-film freeze-drying (TFFD) is a rapid freezing and then drying technique used to prepare inhalable dry powders from the liquid form for applications such as drug delivery to the lungs.					
35259440	1	3	theme	Thin-film	93:101	arg1	TFFD					118:121	TFFD	118:121	TFFD	118:121	Thin-film freeze-drying (TFFD) is a rapid freezing and then drying technique used to prepare inhalable dry powders from the liquid form for applications such as drug delivery to the lungs.					
35259440	10	4	theme	anti-PD-1	1550:1558	arg1	mAbs					1560:1563	the anti-PD-1 mAbs	1546:1563	the anti-PD-1 mAbs	1546:1563	The PD-1 binding activity of the anti-PD-1 mAbs after TFFD was not different from before TFFD.					
35259440	1	5	theme	drug	254:257	arg1	delivery					259:266	drug delivery	254:266	drug delivery to the lungs	254:279	Thin-film freeze-drying (TFFD) is a rapid freezing and then drying technique used to prepare inhalable dry powders from the liquid form for applications such as drug delivery to the lungs.					
35259440	16	6	theme	dry	2383:2385	arg1	powders					2387:2393	stable, aerosolizable dry powders	2361:2393	stable, aerosolizable dry powders of mAbs for pulmonary delivery	2361:2424	We conclude that TFFD can be applied to produce stable, aerosolizable dry powders of mAbs for pulmonary delivery and that formulations must be optimized to maximize aerosol performance and minimize protein aggregation.					
35259440	13	7	theme	total	2055:2059	arg1	count					2082:2086	the total subvisible aggregate count	2051:2086	the total subvisible aggregate count	2051:2086	More subvisible mAb aggregates were generated when PBS was used, but the mAb content in the dry powders did not significantly affect the total subvisible aggregate count.					
35259440	14	8	theme	aggregates	2178:2187	arg1	aggregates					2178:2187	subvisible mAb aggregates	2163:2187	subvisible mAb aggregates	2163:2187	Powders prepared with mannitol as an excipient showed the least amount of subvisible mAb aggregates.					
35259440	14	8	theme	aggregates	2178:2187	arg1	amount					2153:2158	the least amount	2143:2158	the least amount of subvisible mAb aggregates	2143:2187	Powders prepared with mannitol as an excipient showed the least amount of subvisible mAb aggregates.					
35259440	9	9	theme	storage	1486:1492	arg1	stability					1494:1502	the storage stability	1482:1502	the storage stability of the mAbs	1482:1514	The addition of polyvinylpyrrolidone K40 in the formulation raised the Tg to 152 °C, which is expected to further increase the storage stability of the mAbs.					
35259440	4	10	theme	phosphate	587:595	arg1	PBS					614:616	PBS	614:616	PBS	614:616	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	4	10	theme	phosphate	587:595	arg1	IgG-1-LL-PBS					620:631	IgG-1-LL-PBS	620:631	IgG-1-LL-PBS	620:631	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	4	10	theme	phosphate	587:595	arg1	saline					606:611	phosphate buffered saline	587:611	phosphate buffered saline (PBS) (IgG-1-LL-PBS)	587:632	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	5	11	theme	IgG-1-LL-PBS	904:915	arg1	powder					917:922	the IgG-1-LL-PBS powder	900:922	the IgG-1-LL-PBS powder	900:922	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	5	12	theme	death	774:778	arg1	protein					780:786	anti-programmed cell death protein	753:786	anti-programmed cell death protein (anti-PD-1 mAb)	753:802	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	5	12	theme	death	774:778	arg1	mAb					799:801	anti-PD-1 mAb	789:801	anti-PD-1 mAb	789:801	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	5	12	theme	death	774:778	arg1	antibody					816:823	a specific antibody	805:823	a specific antibody	805:823	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	7	13	with	structure	1148:1156	arg1	nanoaggregates					1163:1176	nanoaggregates	1163:1176	nanoaggregates	1163:1176	The TFFD dry powder had a porous structure with nanoaggregates and had a Tg value between 39 and 50 °C.					
35259440	1	14	theme	dry	196:198	arg1	powders					200:206	inhalable dry powders	186:206	inhalable dry powders from the liquid form	186:227	Thin-film freeze-drying (TFFD) is a rapid freezing and then drying technique used to prepare inhalable dry powders from the liquid form for applications such as drug delivery to the lungs.					
35259440	9	15	theme	mAbs	1511:1514	arg1	stability					1494:1502	the storage stability	1482:1502	the storage stability of the mAbs	1482:1514	The addition of polyvinylpyrrolidone K40 in the formulation raised the Tg to 152 °C, which is expected to further increase the storage stability of the mAbs.					
35259440	16	16	theme	mAbs	2398:2401	arg1	powders					2387:2393	stable, aerosolizable dry powders	2361:2393	stable, aerosolizable dry powders of mAbs for pulmonary delivery	2361:2424	We conclude that TFFD can be applied to produce stable, aerosolizable dry powders of mAbs for pulmonary delivery and that formulations must be optimized to maximize aerosol performance and minimize protein aggregation.					
35259440	11	17	theme	protein	1618:1624	arg1	loss					1626:1629	protein loss	1618:1629	protein loss	1618:1629	While protein loss, likely due to protein binding to vials and the thin-film freezing apparatus, was identified, we were able to minimize the loss by increasing the mAb concentration (i.e., from 1% to 13.2%).					
35259440	7	18	contain	had	1182:1184	arg1	powder					1128:1133	The TFFD dry powder	1115:1133	The TFFD dry powder	1115:1133	The TFFD dry powder had a porous structure with nanoaggregates and had a Tg value between 39 and 50 °C.					
35259440	7	18	contain	had	1182:1184	arg2	value					1191:1195	a Tg value	1186:1195	a Tg value between 39 and 50 °C	1186:1216	The TFFD dry powder had a porous structure with nanoaggregates and had a Tg value between 39 and 50 °C.					
35259440	11	19	theme	13.2	1813:1816	arg1	%					1808:1808	%	1808:1808	%	1808:1808	While protein loss, likely due to protein binding to vials and the thin-film freezing apparatus, was identified, we were able to minimize the loss by increasing the mAb concentration (i.e., from 1% to 13.2%).					
35259440	5	20	theme	specific	807:814	arg1	antibody					816:823	a specific antibody	805:823	a specific antibody	805:823	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	5	20	theme	specific	807:814	arg1	protein					780:786	anti-programmed cell death protein	753:786	anti-programmed cell death protein (anti-PD-1 mAb)	753:802	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	6	21	theme	shelf	1074:1078	arg1	freeze-drying					1080:1092	conventional shelf freeze-drying	1061:1092	conventional shelf freeze-drying (shelf FD)	1061:1103	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	6	21	theme	shelf	1074:1078	arg1	FD					1101:1102	shelf FD	1095:1102	shelf FD	1095:1102	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	9	22	from	addition	1363:1370	arg1	formulation					1407:1417	the formulation	1403:1417	the formulation	1403:1417	The addition of polyvinylpyrrolidone K40 in the formulation raised the Tg to 152 °C, which is expected to further increase the storage stability of the mAbs.					
35259440	1	23	theme	drying	153:158	arg1	technique					160:168	a rapid freezing and then drying technique	127:168	technique	160:168	Thin-film freeze-drying (TFFD) is a rapid freezing and then drying technique used to prepare inhalable dry powders from the liquid form for applications such as drug delivery to the lungs.					
35259440	8	24	theme	same	1322:1325	arg1	formulation					1327:1337	the same formulation	1318:1337	the same formulation stored as a liquid	1318:1356	When stored at room temperature, the anti-PD-1 mAb in the TFFD powder was more stable than that of the same formulation stored as a liquid.					
35259440	8	25	theme	anti-PD-1	1256:1264	arg1	stable					1298:1303	stable	1298:1303	stable	1298:1303	When stored at room temperature, the anti-PD-1 mAb in the TFFD powder was more stable than that of the same formulation stored as a liquid.					
35259440	8	25	theme	anti-PD-1	1256:1264	arg1	mAb					1266:1268	the anti-PD-1 mAb	1252:1268	the anti-PD-1 mAb in the TFFD powder	1252:1287	When stored at room temperature, the anti-PD-1 mAb in the TFFD powder was more stable than that of the same formulation stored as a liquid.					
35259440	4	26	with	powder	681:686	arg1	properties					711:720	desirable aerosol properties	693:720	desirable aerosol properties	693:720	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	11	27	theme	mAb	1777:1779	arg1	concentration					1781:1793	the mAb concentration	1773:1793	the mAb concentration (i.e., from 1% to 13.2%)	1773:1818	While protein loss, likely due to protein binding to vials and the thin-film freezing apparatus, was identified, we were able to minimize the loss by increasing the mAb concentration (i.e., from 1% to 13.2%).					
35259440	5	28	theme	dry	839:841	arg1	anti-PD1-1-LL-PBS					851:867	anti-PD1-1-LL-PBS	851:867	anti-PD1-1-LL-PBS	851:867	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	5	28	theme	dry	839:841	arg1	powder					843:848	a dry powder	837:848	a dry powder (anti-PD1-1-LL-PBS)	837:868	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	1	29	theme	rapid	129:133	arg1	freeze-drying					103:115	Thin-film freeze-drying	93:115	Thin-film freeze-drying (TFFD)	93:122	Thin-film freeze-drying (TFFD) is a rapid freezing and then drying technique used to prepare inhalable dry powders from the liquid form for applications such as drug delivery to the lungs.					
35259440	1	29	theme	rapid	129:133	arg1	freezing					135:142	a rapid freezing and then drying technique	127:168	freezing	135:142	Thin-film freeze-drying (TFFD) is a rapid freezing and then drying technique used to prepare inhalable dry powders from the liquid form for applications such as drug delivery to the lungs.					
35259440	11	30	theme	thin-film	1679:1687	arg1	apparatus					1698:1706	the thin-film freezing apparatus	1675:1706	the thin-film freezing apparatus	1675:1706	While protein loss, likely due to protein binding to vials and the thin-film freezing apparatus, was identified, we were able to minimize the loss by increasing the mAb concentration (i.e., from 1% to 13.2%).					
35259440	13	31	used	used	1977:1980	arg2	PBS					1969:1971	PBS	1969:1971	PBS	1969:1971	More subvisible mAb aggregates were generated when PBS was used, but the mAb content in the dry powders did not significantly affect the total subvisible aggregate count.					
35259440	7	32	theme	Tg	1188:1189	arg1	value					1191:1195	a Tg value	1186:1195	a Tg value between 39 and 50 °C	1186:1216	The TFFD dry powder had a porous structure with nanoaggregates and had a Tg value between 39 and 50 °C.					
35259440	0	33	theme	Dry	0:2	arg1	powders					4:10	Dry powders	0:10	Dry powders for inhalation containing monoclonal antibodies	0:58	Dry powders for inhalation containing monoclonal antibodies made by thin-film freeze-drying.					
35259440	9	34	theme	K40	1396:1398	arg1	addition					1363:1370	The addition	1359:1370	The addition of polyvinylpyrrolidone K40 in the formulation	1359:1417	The addition of polyvinylpyrrolidone K40 in the formulation raised the Tg to 152 °C, which is expected to further increase the storage stability of the mAbs.					
35259440	6	35	theme	aerosol	929:935	arg1	properties					937:946	The aerosol properties	925:946	The aerosol properties of the anti-PD1-1-LL-PBS dry powder	925:982	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	6	35	theme	aerosol	929:935	arg1	better					1003:1008	better	1003:1008	better	1003:1008	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	16	36	theme	pulmonary	2407:2415	arg1	delivery					2417:2424	pulmonary delivery	2407:2424	pulmonary delivery	2407:2424	We conclude that TFFD can be applied to produce stable, aerosolizable dry powders of mAbs for pulmonary delivery and that formulations must be optimized to maximize aerosol performance and minimize protein aggregation.					
35259440	6	37	used	used	1024:1027	arg2	TFFD					1015:1018	TFFD	1015:1018	TFFD	1015:1018	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	8	38	theme	room	1234:1237	arg1	temperature					1239:1249	room temperature	1234:1249	room temperature	1234:1249	When stored at room temperature, the anti-PD-1 mAb in the TFFD powder was more stable than that of the same formulation stored as a liquid.					
35259440	0	39	theme	containing	27:36	arg1	antibodies					49:58	inhalation containing monoclonal antibodies	16:58	inhalation containing monoclonal antibodies	16:58	Dry powders for inhalation containing monoclonal antibodies made by thin-film freeze-drying.					
35259440	14	40	theme	subvisible	2163:2172	arg1	aggregates					2178:2187	subvisible mAb aggregates	2163:2187	subvisible mAb aggregates	2163:2187	Powders prepared with mannitol as an excipient showed the least amount of subvisible mAb aggregates.					
35259440	11	41	theme	due	1639:1641	arg1	loss					1626:1629	protein loss	1618:1629	protein loss	1618:1629	While protein loss, likely due to protein binding to vials and the thin-film freezing apparatus, was identified, we were able to minimize the loss by increasing the mAb concentration (i.e., from 1% to 13.2%).					
35259440	4	42	dep	lactose/leucine	555:569	arg1	60:40					572:576	60:40	572:576	60:40	572:576	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	4	42	dep	lactose/leucine	555:569	arg1	w/w					579:581	w/w	579:581	w/w	579:581	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	16	43	theme	aerosol	2478:2484	arg1	performance					2486:2496	aerosol performance	2478:2496	aerosol performance	2478:2496	We conclude that TFFD can be applied to produce stable, aerosolizable dry powders of mAbs for pulmonary delivery and that formulations must be optimized to maximize aerosol performance and minimize protein aggregation.					
35259440	11	44	theme	protein	1646:1652	arg1	binding					1654:1660	protein binding	1646:1660	protein binding to vials and the thin-film freezing apparatus	1646:1706	While protein loss, likely due to protein binding to vials and the thin-film freezing apparatus, was identified, we were able to minimize the loss by increasing the mAb concentration (i.e., from 1% to 13.2%).					
35259440	4	45	theme	desirable	693:701	arg1	properties					711:720	desirable aerosol properties	693:720	desirable aerosol properties	693:720	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	2	46	theme	monoclonal	347:356	arg1	mAbs					370:373	mAbs	370:373	mAbs	370:373	Herein we report the preparation of aerosolizable dry powders of monoclonal antibodies (mAbs) by TFFD.					
35259440	2	46	theme	monoclonal	347:356	arg1	antibodies					358:367	monoclonal antibodies	347:367	monoclonal antibodies (mAbs)	347:374	Herein we report the preparation of aerosolizable dry powders of monoclonal antibodies (mAbs) by TFFD.					
35259440	5	47	theme	anti-programmed	753:767	arg1	protein					780:786	anti-programmed cell death protein	753:786	anti-programmed cell death protein (anti-PD-1 mAb)	753:802	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	5	47	theme	anti-programmed	753:767	arg1	mAb					799:801	anti-PD-1 mAb	789:801	anti-PD-1 mAb	789:801	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	5	47	theme	anti-programmed	753:767	arg1	antibody					816:823	a specific antibody	805:823	a specific antibody	805:823	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	13	48	theme	subvisible	1923:1932	arg1	aggregates					1938:1947	More subvisible mAb aggregates	1918:1947	More subvisible mAb aggregates	1918:1947	More subvisible mAb aggregates were generated when PBS was used, but the mAb content in the dry powders did not significantly affect the total subvisible aggregate count.					
35259440	13	49	theme	aggregate	2072:2080	arg1	count					2082:2086	the total subvisible aggregate count	2051:2086	the total subvisible aggregate count	2051:2086	More subvisible mAb aggregates were generated when PBS was used, but the mAb content in the dry powders did not significantly affect the total subvisible aggregate count.					
35259440	7	50	theme	TFFD	1119:1122	arg1	powder					1128:1133	The TFFD dry powder	1115:1133	The TFFD dry powder	1115:1133	The TFFD dry powder had a porous structure with nanoaggregates and had a Tg value between 39 and 50 °C.					
35259440	11	51	dep	%	1808:1808	arg1	to					1810:1811	to	1810:1811	to	1810:1811	While protein loss, likely due to protein binding to vials and the thin-film freezing apparatus, was identified, we were able to minimize the loss by increasing the mAb concentration (i.e., from 1% to 13.2%).					
35259440	2	52	theme	powders	336:342	arg1	preparation					303:313	the preparation	299:313	the preparation of aerosolizable dry powders of monoclonal antibodies (mAbs) by TFFD	299:382	Herein we report the preparation of aerosolizable dry powders of monoclonal antibodies (mAbs) by TFFD.					
35259440	6	53	theme	anti-PD1-1-LL-PBS	955:971	arg1	powder					977:982	the anti-PD1-1-LL-PBS dry powder	951:982	the anti-PD1-1-LL-PBS dry powder	951:982	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	13	54	from	content	1995:2001	arg1	powders					2014:2020	the dry powders	2006:2020	the dry powders	2006:2020	More subvisible mAb aggregates were generated when PBS was used, but the mAb content in the dry powders did not significantly affect the total subvisible aggregate count.					
35259440	10	55	theme	mAbs	1560:1563	arg1	different					1584:1592	different	1584:1592	different	1584:1592	The PD-1 binding activity of the anti-PD-1 mAbs after TFFD was not different from before TFFD.					
35259440	10	55	theme	mAbs	1560:1563	arg1	activity					1534:1541	The PD-1 binding activity	1517:1541	The PD-1 binding activity of the anti-PD-1 mAbs after TFFD	1517:1574	The PD-1 binding activity of the anti-PD-1 mAbs after TFFD was not different from before TFFD.					
35259440	2	56	theme	aerosolizable	318:330	arg1	powders					336:342	aerosolizable dry powders	318:342	aerosolizable dry powders of monoclonal antibodies (mAbs)	318:374	Herein we report the preparation of aerosolizable dry powders of monoclonal antibodies (mAbs) by TFFD.					
35259440	5	57	theme	cell	769:772	arg1	protein					780:786	anti-programmed cell death protein	753:786	anti-programmed cell death protein (anti-PD-1 mAb)	753:802	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	5	57	theme	cell	769:772	arg1	mAb					799:801	anti-PD-1 mAb	789:801	anti-PD-1 mAb	789:801	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	5	57	theme	cell	769:772	arg1	antibody					816:823	a specific antibody	805:823	a specific antibody	805:823	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	16	58	theme	protein	2511:2517	arg1	aggregation					2519:2529	protein aggregation	2511:2529	protein aggregation	2511:2529	We conclude that TFFD can be applied to produce stable, aerosolizable dry powders of mAbs for pulmonary delivery and that formulations must be optimized to maximize aerosol performance and minimize protein aggregation.					
35259440	13	59	theme	subvisible	2061:2070	arg1	count					2082:2086	the total subvisible aggregate count	2051:2086	the total subvisible aggregate count	2051:2086	More subvisible mAb aggregates were generated when PBS was used, but the mAb content in the dry powders did not significantly affect the total subvisible aggregate count.					
35259440	3	60	theme	aerosol	501:507	arg1	performance					509:519	their aerosol performance	495:519	their aerosol performance	495:519	We first formulated an IgG antibody with lactose/leucine (60:40, w/w) or trehalose/leucine (75:25) and tested their aerosol performance.					
35259440	8	61	from	mAb	1266:1268	arg1	powder					1282:1287	the TFFD powder	1273:1287	the TFFD powder	1273:1287	When stored at room temperature, the anti-PD-1 mAb in the TFFD powder was more stable than that of the same formulation stored as a liquid.					
35259440	1	62	theme	inhalable	186:194	arg1	powders					200:206	inhalable dry powders	186:206	inhalable dry powders from the liquid form	186:227	Thin-film freeze-drying (TFFD) is a rapid freezing and then drying technique used to prepare inhalable dry powders from the liquid form for applications such as drug delivery to the lungs.					
35259440	8	63	theme	TFFD	1277:1280	arg1	powder					1282:1287	the TFFD powder	1273:1287	the TFFD powder	1273:1287	When stored at room temperature, the anti-PD-1 mAb in the TFFD powder was more stable than that of the same formulation stored as a liquid.					
35259440	12	64	theme	Micro-flow	1821:1830	arg1	imaging					1832:1838	Micro-flow imaging	1821:1838	Micro-flow imaging	1821:1838	Micro-flow imaging revealed that the excipients and PBS affected subvisible aggregate formation.					
35259440	4	65	from	lactose/leucine	555:569	arg1	PBS					614:616	PBS	614:616	PBS	614:616	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	4	65	from	lactose/leucine	555:569	arg1	IgG-1-LL-PBS					620:631	IgG-1-LL-PBS	620:631	IgG-1-LL-PBS	620:631	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	4	65	from	lactose/leucine	555:569	arg1	saline					606:611	phosphate buffered saline	587:611	phosphate buffered saline (PBS) (IgG-1-LL-PBS)	587:632	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	11	66	theme	1	1807:1807	arg1	%					1808:1808	%	1808:1808	%	1808:1808	While protein loss, likely due to protein binding to vials and the thin-film freezing apparatus, was identified, we were able to minimize the loss by increasing the mAb concentration (i.e., from 1% to 13.2%).					
35259440	1	67	from	form	224:227	arg1	powders					200:206	inhalable dry powders	186:206	inhalable dry powders from the liquid form	186:227	Thin-film freeze-drying (TFFD) is a rapid freezing and then drying technique used to prepare inhalable dry powders from the liquid form for applications such as drug delivery to the lungs.					
35259440	4	68	dep	IgG	526:528	arg1	%					531:531	1%	530:531	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD	522:654	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	4	68	dep	IgG	526:528	arg1	formulated					539:548	formulated	539:548	formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS)	539:632	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	4	68	dep	IgG	526:528	arg1	processed					638:646	processed	638:646	processed by TFFD	638:654	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	4	68	dep	IgG	526:528	arg1	w/w					534:536	w/w	534:536	w/w	534:536	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	6	69	theme	shelf	1095:1099	arg1	freeze-drying					1080:1092	conventional shelf freeze-drying	1061:1092	conventional shelf freeze-drying (shelf FD)	1061:1103	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	6	69	theme	shelf	1095:1099	arg1	FD					1101:1102	shelf FD	1095:1102	shelf FD	1095:1102	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	12	70	theme	subvisible	1886:1895	arg1	formation					1907:1915	subvisible aggregate formation	1886:1915	subvisible aggregate formation	1886:1915	Micro-flow imaging revealed that the excipients and PBS affected subvisible aggregate formation.					
35259440	13	71	theme	dry	2010:2012	arg1	powders					2014:2020	the dry powders	2006:2020	the dry powders	2006:2020	More subvisible mAb aggregates were generated when PBS was used, but the mAb content in the dry powders did not significantly affect the total subvisible aggregate count.					
35259440	6	72	used	used	1109:1112	arg2	FD					1101:1102	shelf FD	1095:1102	shelf FD	1095:1102	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	6	72	used	used	1109:1112	arg2	freeze-drying					1080:1092	conventional shelf freeze-drying	1061:1092	conventional shelf freeze-drying (shelf FD)	1061:1103	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	0	73	theme	thin-film	68:76	arg1	freeze-drying					78:90	thin-film freeze-drying	68:90	thin-film freeze-drying	68:90	Dry powders for inhalation containing monoclonal antibodies made by thin-film freeze-drying.					
35259440	6	74	theme	conventional	1061:1072	arg1	freeze-drying					1080:1092	conventional shelf freeze-drying	1061:1092	conventional shelf freeze-drying (shelf FD)	1061:1103	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	6	74	theme	conventional	1061:1072	arg1	FD					1101:1102	shelf FD	1095:1102	shelf FD	1095:1102	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	4	75	theme	buffered	597:604	arg1	PBS					614:616	PBS	614:616	PBS	614:616	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	4	75	theme	buffered	597:604	arg1	IgG-1-LL-PBS					620:631	IgG-1-LL-PBS	620:631	IgG-1-LL-PBS	620:631	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	4	75	theme	buffered	597:604	arg1	saline					606:611	phosphate buffered saline	587:611	phosphate buffered saline (PBS) (IgG-1-LL-PBS)	587:632	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	16	76	theme	stable	2361:2366	arg1	powders					2387:2393	stable, aerosolizable dry powders	2361:2393	stable, aerosolizable dry powders of mAbs for pulmonary delivery	2361:2424	We conclude that TFFD can be applied to produce stable, aerosolizable dry powders of mAbs for pulmonary delivery and that formulations must be optimized to maximize aerosol performance and minimize protein aggregation.					
35259440	13	77	theme	mAb	1991:1993	arg1	content					1995:2001	the mAb content	1987:2001	the mAb content in the dry powders	1987:2020	More subvisible mAb aggregates were generated when PBS was used, but the mAb content in the dry powders did not significantly affect the total subvisible aggregate count.					
35259440	11	78	theme	freezing	1689:1696	arg1	apparatus					1698:1706	the thin-film freezing apparatus	1675:1706	the thin-film freezing apparatus	1675:1706	While protein loss, likely due to protein binding to vials and the thin-film freezing apparatus, was identified, we were able to minimize the loss by increasing the mAb concentration (i.e., from 1% to 13.2%).					
35259440	7	79	contain	had	1135:1137	arg1	powder					1128:1133	The TFFD dry powder	1115:1133	The TFFD dry powder	1115:1133	The TFFD dry powder had a porous structure with nanoaggregates and had a Tg value between 39 and 50 °C.					
35259440	7	79	contain	had	1135:1137	arg2	structure					1148:1156	a porous structure	1139:1156	a porous structure with nanoaggregates	1139:1176	The TFFD dry powder had a porous structure with nanoaggregates and had a Tg value between 39 and 50 °C.					
35259440	9	80	theme	polyvinylpyrrolidone	1375:1394	arg1	K40					1396:1398	polyvinylpyrrolidone K40	1375:1398	polyvinylpyrrolidone K40	1375:1398	The addition of polyvinylpyrrolidone K40 in the formulation raised the Tg to 152 °C, which is expected to further increase the storage stability of the mAbs.					
35259440	2	81	theme	antibodies	358:367	arg1	powders					336:342	aerosolizable dry powders	318:342	aerosolizable dry powders of monoclonal antibodies (mAbs)	318:374	Herein we report the preparation of aerosolizable dry powders of monoclonal antibodies (mAbs) by TFFD.					
35259440	0	82	theme	inhalation	16:25	arg1	antibodies					49:58	inhalation containing monoclonal antibodies	16:58	inhalation containing monoclonal antibodies	16:58	Dry powders for inhalation containing monoclonal antibodies made by thin-film freeze-drying.					
35259440	5	83	with	IgG	744:746	arg1	protein					780:786	anti-programmed cell death protein	753:786	anti-programmed cell death protein (anti-PD-1 mAb)	753:802	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	5	83	with	IgG	744:746	arg1	mAb					799:801	anti-PD-1 mAb	789:801	anti-PD-1 mAb	789:801	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	5	83	with	IgG	744:746	arg1	antibody					816:823	a specific antibody	805:823	a specific antibody	805:823	We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder.					
35259440	0	84	theme	monoclonal	38:47	arg1	antibodies					49:58	inhalation containing monoclonal antibodies	16:58	inhalation containing monoclonal antibodies	16:58	Dry powders for inhalation containing monoclonal antibodies made by thin-film freeze-drying.					
35259440	7	85	theme	porous	1141:1146	arg1	structure					1148:1156	a porous structure	1139:1156	a porous structure with nanoaggregates	1139:1176	The TFFD dry powder had a porous structure with nanoaggregates and had a Tg value between 39 and 50 °C.					
35259440	4	86	theme	aerosol	703:709	arg1	properties					711:720	desirable aerosol properties	693:720	desirable aerosol properties	693:720	The IgG 1% (w/w) formulated with lactose/leucine (60:40, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties.					
35259440	14	87	theme	mAb	2174:2176	arg1	aggregates					2178:2187	subvisible mAb aggregates	2163:2187	subvisible mAb aggregates	2163:2187	Powders prepared with mannitol as an excipient showed the least amount of subvisible mAb aggregates.					
35259440	15	88	theme	similar	2284:2290	arg1	composition					2292:2302	a similar composition	2282:2302	a similar composition by TFFD	2282:2310	Finally, we showed that anti-TNF-α, another mAb, can also be converted to a dry powder with a similar composition by TFFD.					
35259440	7	89	theme	dry	1124:1126	arg1	powder					1128:1133	The TFFD dry powder	1115:1133	The TFFD dry powder	1115:1133	The TFFD dry powder had a porous structure with nanoaggregates and had a Tg value between 39 and 50 °C.					
35259440	16	90	dep	stable	2361:2366	arg1	aerosolizable					2369:2381	aerosolizable	2369:2381	aerosolizable	2369:2381	We conclude that TFFD can be applied to produce stable, aerosolizable dry powders of mAbs for pulmonary delivery and that formulations must be optimized to maximize aerosol performance and minimize protein aggregation.					
35259440	14	91	theme	least	2147:2151	arg1	aggregates					2178:2187	subvisible mAb aggregates	2163:2187	subvisible mAb aggregates	2163:2187	Powders prepared with mannitol as an excipient showed the least amount of subvisible mAb aggregates.					
35259440	14	91	theme	least	2147:2151	arg1	amount					2153:2158	the least amount	2143:2158	the least amount of subvisible mAb aggregates	2143:2187	Powders prepared with mannitol as an excipient showed the least amount of subvisible mAb aggregates.					
35259440	6	92	theme	powder	977:982	arg1	properties					937:946	The aerosol properties	925:946	The aerosol properties of the anti-PD1-1-LL-PBS dry powder	925:982	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	6	92	theme	powder	977:982	arg1	better					1003:1008	better	1003:1008	better	1003:1008	The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used.					
35259440	1	93	theme	liquid	217:222	arg1	form					224:227	the liquid form	213:227	the liquid form	213:227	Thin-film freeze-drying (TFFD) is a rapid freezing and then drying technique used to prepare inhalable dry powders from the liquid form for applications such as drug delivery to the lungs.					
35259440	3	94	dep	lactose/leucine	426:440	arg1	w/w					450:452	w/w	450:452	w/w	450:452	We first formulated an IgG antibody with lactose/leucine (60:40, w/w) or trehalose/leucine (75:25) and tested their aerosol performance.					
35259440	3	94	dep	lactose/leucine	426:440	arg1	60:40					443:447	60:40	443:447	60:40	443:447	We first formulated an IgG antibody with lactose/leucine (60:40, w/w) or trehalose/leucine (75:25) and tested their aerosol performance.					
35259440	10	95	theme	PD-1	1521:1524	arg1	different					1584:1592	different	1584:1592	different	1584:1592	The PD-1 binding activity of the anti-PD-1 mAbs after TFFD was not different from before TFFD.					
35259440	10	95	theme	PD-1	1521:1524	arg1	activity					1534:1541	The PD-1 binding activity	1517:1541	The PD-1 binding activity of the anti-PD-1 mAbs after TFFD	1517:1574	The PD-1 binding activity of the anti-PD-1 mAbs after TFFD was not different from before TFFD.					
35259440	3	96	theme	IgG	408:410	arg1	antibody					412:419	an IgG antibody	405:419	an IgG antibody	405:419	We first formulated an IgG antibody with lactose/leucine (60:40, w/w) or trehalose/leucine (75:25) and tested their aerosol performance.					
35259440	12	97	theme	aggregate	1897:1905	arg1	formation					1907:1915	subvisible aggregate formation	1886:1915	subvisible aggregate formation	1886:1915	Micro-flow imaging revealed that the excipients and PBS affected subvisible aggregate formation.					
35259440	10	98	theme	binding	1526:1532	arg1	different					1584:1592	different	1584:1592	different	1584:1592	The PD-1 binding activity of the anti-PD-1 mAbs after TFFD was not different from before TFFD.					
35259440	10	98	theme	binding	1526:1532	arg1	activity					1534:1541	The PD-1 binding activity	1517:1541	The PD-1 binding activity of the anti-PD-1 mAbs after TFFD	1517:1574	The PD-1 binding activity of the anti-PD-1 mAbs after TFFD was not different from before TFFD.					
35259440	15	99	theme	dry	2266:2268	arg1	powder					2270:2275	a dry powder	2264:2275	a dry powder with a similar composition by TFFD	2264:2310	Finally, we showed that anti-TNF-α, another mAb, can also be converted to a dry powder with a similar composition by TFFD.					
35259440	13	100	theme	mAb	1934:1936	arg1	aggregates					1938:1947	More subvisible mAb aggregates	1918:1947	More subvisible mAb aggregates	1918:1947	More subvisible mAb aggregates were generated when PBS was used, but the mAb content in the dry powders did not significantly affect the total subvisible aggregate count.					
37300561	5	0	from	species	918:924	arg1	isolates					1011:1018	Aspergillus fumigatus isolates	989:1018	Aspergillus fumigatus isolates	989:1018	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	9	1	theme	species	1555:1561	arg1	related					1574:1580	related	1574:1580	related	1574:1580	The majority of bacterial species found were related to the human skin microflora, such as different Staphylococcus and Corynebacterium species.					
37300561	9	1	theme	species	1555:1561	arg1	majority					1533:1540	The majority	1529:1540	The majority of bacterial species found	1529:1567	The majority of bacterial species found were related to the human skin microflora, such as different Staphylococcus and Corynebacterium species.					
37300561	8	2	located	found	1506:1510	arg1	surfaces					1454:1461	surfaces	1454:1461	surfaces	1454:1461	On surfaces, the highest bacterial concentrations were found on bed railings.					
37300561	8	2	located	found	1506:1510	arg2	concentrations					1486:1499	the highest bacterial concentrations	1464:1499	the highest bacterial concentrations	1464:1499	On surfaces, the highest bacterial concentrations were found on bed railings.					
37300561	8	2	located	found	1506:1510	arg1	railings					1519:1526	bed railings	1515:1526	bed railings	1515:1526	On surfaces, the highest bacterial concentrations were found on bed railings.					
37300561	6	3	from	samples	1069:1075	arg1	concentrations					1031:1044	Microbial concentrations	1021:1044	Microbial concentrations from personal exposure samples	1021:1075	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	5	4	dep	Aspergillus	989:999	arg1	fumigatus					1001:1009	fumigatus	1001:1009	fumigatus	1001:1009	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	4	5	from	groups	629:634	arg1	samples					606:612	personal bioaerosol samples	586:612	personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands	586:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	4	5	from	groups	629:634	arg1	use					579:581	use	579:581	use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands	579:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	4	6	theme	staff	639:643	arg1	members					645:651	staff members	639:651	staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands	639:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	6	7	theme	nutrient	1192:1199	arg1	agar					1201:1204	nutrient agar	1192:1204	nutrient agar	1192:1204	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	11	8	theme	amphotericin	1918:1929	arg1	B					1931:1931	amphotericin B	1918:1931	amphotericin B	1918:1931	Of 40 tested A. fumigatus isolates, we found one multiresistant isolate, which was resistant towards both itraconazole and voriconazole, and one isolate resistant towards amphotericin B.					
37300561	9	9	theme	bacterial	1545:1553	arg1	species					1555:1561	bacterial species	1545:1561	bacterial species found	1545:1567	The majority of bacterial species found were related to the human skin microflora, such as different Staphylococcus and Corynebacterium species.					
37300561	9	10	theme	human	1589:1593	arg1	microflora					1600:1609	the human skin microflora	1585:1609	the human skin microflora	1585:1609	The majority of bacterial species found were related to the human skin microflora, such as different Staphylococcus and Corynebacterium species.					
37300561	9	10	theme	human	1589:1593	arg1	species					1665:1671	different Staphylococcus and Corynebacterium species	1620:1671	different Staphylococcus and Corynebacterium species	1620:1671	The majority of bacterial species found were related to the human skin microflora, such as different Staphylococcus and Corynebacterium species.					
37300561	4	11	theme	bioaerosol	595:604	arg1	samples					606:612	personal bioaerosol samples	586:612	personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands	586:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	10	12	theme	Endotoxin	1674:1682	arg1	levels					1684:1689	Endotoxin levels	1674:1689	Endotoxin levels	1674:1689	Endotoxin levels ranged from 0.02 to 59.0 EU/m3, with a GM of 1.5 EU/m3.					
37300561	6	13	dep	1.5	1155:1157	arg1	to					1152:1153	to	1152:1153	to	1152:1153	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	12	14	theme	general	1976:1982	arg1	exposure					1994:2001	the general microbial exposure	1972:2001	the general microbial exposure	1972:2001	In conclusion, we give an overview of the general microbial exposure in nursing homes and show that microbial exposures are higher for staff with more care and nursing tasks compared with administrative staff.					
37300561	2	15	theme	nursing	222:228	arg1	homes					230:234	nursing homes	222:234	nursing homes	222:234	Only little is known about the occupational microbial exposure in nursing homes and the factors that influence the exposure.					
37300561	4	16	theme	working	676:682	arg1	measurements					711:722	stationary bioaerosol measurements	689:722	stationary bioaerosol measurements taken during various work tasks	689:754	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	4	16	theme	working	676:682	arg1	day					684:686	a typical working day	666:686	a typical working day	666:686	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	9	17	theme	different	1620:1628	arg1	species					1665:1671	different Staphylococcus and Corynebacterium species	1620:1671	different Staphylococcus and Corynebacterium species	1620:1671	The majority of bacterial species found were related to the human skin microflora, such as different Staphylococcus and Corynebacterium species.					
37300561	5	18	dep	species	918:924	arg1	composition					926:936	composition	926:936	composition	926:936	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	5	18	dep	species	918:924	arg1	levels					949:954	endotoxin levels	939:954	endotoxin levels	939:954	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	5	18	dep	species	918:924	arg1	species					918:924	species composition, endotoxin levels, and antimicrobial resistance	918:984	species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates	918:1018	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	5	18	dep	species	918:924	arg1	resistance					975:984	antimicrobial resistance	961:984	antimicrobial resistance	961:984	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	5	19	theme	bacterial	878:886	arg1	concentrations					899:912	bacterial and fungal concentrations	878:912	bacterial and fungal concentrations	878:912	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	6	20	theme	16	1302:1303	arg1	cfu/m3					1305:1310	cfu/m3	1305:1310	cfu/m3	1305:1310	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	1	21	theme	health	140:145	arg1	effects					147:153	negative health effects	131:153	negative health effects	131:153	Indoor microbial exposure may cause negative health effects.					
37300561	0	22	theme	bacterial	65:73	arg1	species					86:92	and bacterial and fungal species	61:92	and bacterial and fungal species	61:92	Work in nursing homes and occupational exposure to endotoxin and bacterial and fungal species.					
37300561	10	23	dep	59.0	1711:1714	arg1	to					1708:1709	to	1708:1709	to	1708:1709	Endotoxin levels ranged from 0.02 to 59.0 EU/m3, with a GM of 1.5 EU/m3.					
37300561	3	24	theme	close	335:339	arg1	contact					341:347	close contact	335:347	close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms	335:435	The exposure in nursing homes may be increased due to close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms and due to handling of laundry, such as used clothing and bed linen.					
37300561	0	25	from	Work	0:3	arg1	homes					16:20	nursing homes	8:20	nursing homes	8:20	Work in nursing homes and occupational exposure to endotoxin and bacterial and fungal species.					
37300561	0	25	from	Work	0:3	arg1	exposure					39:46	occupational exposure	26:46	occupational exposure to endotoxin	26:59	Work in nursing homes and occupational exposure to endotoxin and bacterial and fungal species.					
37300561	4	26	theme	various	737:743	arg1	tasks					750:754	various work tasks	737:754	various work tasks	737:754	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	11	27	dep	A.	1760:1761	arg1	fumigatus					1763:1771	fumigatus	1763:1771	fumigatus	1763:1771	Of 40 tested A. fumigatus isolates, we found one multiresistant isolate, which was resistant towards both itraconazole and voriconazole, and one isolate resistant towards amphotericin B.					
37300561	0	28	theme	fungal	79:84	arg1	species					86:92	and bacterial and fungal species	61:92	and bacterial and fungal species	61:92	Work in nursing homes and occupational exposure to endotoxin and bacterial and fungal species.					
37300561	3	29	dep	such	469:472	arg1	used					477:480	used	477:480	used clothing and bed linen	477:503	The exposure in nursing homes may be increased due to close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms and due to handling of laundry, such as used clothing and bed linen.					
37300561	0	30	theme	occupational	26:37	arg1	exposure					39:46	occupational exposure	26:46	occupational exposure to endotoxin	26:59	Work in nursing homes and occupational exposure to endotoxin and bacterial and fungal species.					
37300561	5	31	theme	endotoxin	939:947	arg1	levels					949:954	endotoxin levels	939:954	endotoxin levels	939:954	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	6	32	theme	exposure	1060:1067	arg1	samples					1069:1075	personal exposure samples	1051:1075	personal exposure samples	1051:1075	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	12	33	theme	administrative	2122:2135	arg1	staff					2137:2141	administrative staff	2122:2141	administrative staff	2122:2141	In conclusion, we give an overview of the general microbial exposure in nursing homes and show that microbial exposures are higher for staff with more care and nursing tasks compared with administrative staff.					
37300561	4	34	theme	bioaerosol	700:709	arg1	measurements					711:722	stationary bioaerosol measurements	689:722	stationary bioaerosol measurements taken during various work tasks	689:754	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	4	34	theme	bioaerosol	700:709	arg1	day					684:686	a typical working day	666:686	a typical working day	666:686	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	3	35	theme	antimicrobial-resistant	398:420	arg1	microorganisms					422:435	infectious or antimicrobial-resistant microorganisms	384:435	microorganisms	422:435	The exposure in nursing homes may be increased due to close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms and due to handling of laundry, such as used clothing and bed linen.					
37300561	5	36	theme	fungal	892:897	arg1	concentrations					899:912	bacterial and fungal concentrations	878:912	bacterial and fungal concentrations	878:912	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	4	37	theme	microbial	526:534	arg1	exposure					536:543	the microbial exposure	522:543	the microbial exposure	522:543	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	12	38	theme	more	2080:2083	arg1	care					2085:2088	more care	2080:2088	more care	2080:2088	In conclusion, we give an overview of the general microbial exposure in nursing homes and show that microbial exposures are higher for staff with more care and nursing tasks compared with administrative staff.					
37300561	11	39	theme	multiresistant	1796:1809	arg1	isolate					1811:1817	one multiresistant isolate	1792:1817	one multiresistant isolate	1792:1817	Of 40 tested A. fumigatus isolates, we found one multiresistant isolate, which was resistant towards both itraconazole and voriconazole, and one isolate resistant towards amphotericin B.					
37300561	0	40	theme	nursing	8:14	arg1	homes					16:20	nursing homes	8:20	nursing homes	8:20	Work in nursing homes and occupational exposure to endotoxin and bacterial and fungal species.					
37300561	1	41	theme	Indoor	95:100	arg1	exposure					112:119	Indoor microbial exposure	95:119	Indoor microbial exposure	95:119	Indoor microbial exposure may cause negative health effects.					
37300561	6	42	theme	Microbial	1021:1029	arg1	concentrations					1031:1044	Microbial concentrations	1021:1044	Microbial concentrations from personal exposure samples	1021:1075	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	3	43	theme	infectious	384:393	arg1	microorganisms					422:435	infectious or antimicrobial-resistant microorganisms	384:435	microorganisms	422:435	The exposure in nursing homes may be increased due to close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms and due to handling of laundry, such as used clothing and bed linen.					
37300561	10	44	theme	EU/m3	1740:1744	arg1	GM					1730:1731	a GM	1728:1731	a GM of 1.5 EU/m3	1728:1744	Endotoxin levels ranged from 0.02 to 59.0 EU/m3, with a GM of 1.5 EU/m3.					
37300561	4	45	theme	environmental	782:794	arg1	swabs					804:808	environmental surface swabs	782:808	environmental surface swabs	782:808	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	12	46	theme	exposure	1994:2001	arg1	overview					1960:1967	an overview	1957:1967	an overview of the general microbial exposure in nursing homes	1957:2018	In conclusion, we give an overview of the general microbial exposure in nursing homes and show that microbial exposures are higher for staff with more care and nursing tasks compared with administrative staff.					
37300561	6	47	theme	potential	1320:1328	arg1	fungi					1341:1345	potential pathogenic fungi	1320:1345	potential pathogenic fungi incubated at 37 °C (below detection limit to 257)	1320:1395	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	4	48	from	use	579:581	arg1	groups					629:634	different groups	619:634	different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands	619:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	4	48	from	use	579:581	arg1	members					645:651	staff members	639:651	staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands	639:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	8	49	theme	bacterial	1476:1484	arg1	concentrations					1486:1499	the highest bacterial concentrations	1464:1499	the highest bacterial concentrations	1464:1499	On surfaces, the highest bacterial concentrations were found on bed railings.					
37300561	2	50	theme	occupational	187:198	arg1	exposure					210:217	the occupational microbial exposure	183:217	the occupational microbial exposure in nursing homes	183:234	Only little is known about the occupational microbial exposure in nursing homes and the factors that influence the exposure.					
37300561	3	51	theme	bed	495:497	arg1	linen					499:503	bed linen	495:503	bed linen	495:503	The exposure in nursing homes may be increased due to close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms and due to handling of laundry, such as used clothing and bed linen.					
37300561	12	52	theme	microbial	1984:1992	arg1	exposure					1994:2001	the general microbial exposure	1972:2001	the general microbial exposure	1972:2001	In conclusion, we give an overview of the general microbial exposure in nursing homes and show that microbial exposures are higher for staff with more care and nursing tasks compared with administrative staff.					
37300561	5	53	theme	antimicrobial	961:973	arg1	species					918:924	species composition, endotoxin levels, and antimicrobial resistance	918:984	species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates	918:1018	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	5	53	theme	antimicrobial	961:973	arg1	resistance					975:984	antimicrobial resistance	961:984	antimicrobial resistance	961:984	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	4	54	theme	staff	826:830	arg1	members					832:838	staff members	826:838	staff members' hands	826:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	3	55	contain	carry	378:382	arg1	persons					362:368	elderly persons	354:368	elderly persons who may carry infectious or antimicrobial-resistant microorganisms	354:435	The exposure in nursing homes may be increased due to close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms and due to handling of laundry, such as used clothing and bed linen.					
37300561	3	55	contain	carry	378:382	arg2	microorganisms					422:435	infectious or antimicrobial-resistant microorganisms	384:435	microorganisms	422:435	The exposure in nursing homes may be increased due to close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms and due to handling of laundry, such as used clothing and bed linen.					
37300561	4	56	theme	nursing	550:556	arg1	homes					558:562	5 nursing homes	548:562	5 nursing homes in Denmark	548:573	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	12	57	theme	nursing	2006:2012	arg1	homes					2014:2018	nursing homes	2006:2018	nursing homes	2006:2018	In conclusion, we give an overview of the general microbial exposure in nursing homes and show that microbial exposures are higher for staff with more care and nursing tasks compared with administrative staff.					
37300561	4	58	theme	different	619:627	arg1	groups					629:634	different groups	619:634	different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands	619:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	4	58	theme	different	619:627	arg1	members					645:651	staff members	639:651	staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands	639:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	6	59	dep	2.0	1227:1229	arg1	to					1224:1225	to	1224:1225	to	1224:1225	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	9	60	theme	skin	1595:1598	arg1	microflora					1600:1609	the human skin microflora	1585:1609	the human skin microflora	1585:1609	The majority of bacterial species found were related to the human skin microflora, such as different Staphylococcus and Corynebacterium species.					
37300561	9	60	theme	skin	1595:1598	arg1	species					1665:1671	different Staphylococcus and Corynebacterium species	1620:1671	different Staphylococcus and Corynebacterium species	1620:1671	The majority of bacterial species found were related to the human skin microflora, such as different Staphylococcus and Corynebacterium species.					
37300561	4	61	theme	personal	586:593	arg1	samples					606:612	personal bioaerosol samples	586:612	personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands	586:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	4	62	theme	samples	606:612	arg1	use					579:581	use	579:581	use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands	579:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	1	63	theme	microbial	102:110	arg1	exposure					112:119	Indoor microbial exposure	95:119	Indoor microbial exposure	95:119	Indoor microbial exposure may cause negative health effects.					
37300561	3	64	theme	nursing	297:303	arg1	homes					305:309	nursing homes	297:309	nursing homes	297:309	The exposure in nursing homes may be increased due to close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms and due to handling of laundry, such as used clothing and bed linen.					
37300561	3	65	with	contact	341:347	arg1	persons					362:368	elderly persons	354:368	elderly persons who may carry infectious or antimicrobial-resistant microorganisms	354:435	The exposure in nursing homes may be increased due to close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms and due to handling of laundry, such as used clothing and bed linen.					
37300561	4	66	theme	typical	668:674	arg1	measurements					711:722	stationary bioaerosol measurements	689:722	stationary bioaerosol measurements taken during various work tasks	689:754	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	4	66	theme	typical	668:674	arg1	day					684:686	a typical working day	666:686	a typical working day	666:686	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	6	67	theme	×	1231:1231	arg1	104					1233:1235	82 to 2.0 × 104	1221:1235	82 to 2.0 × 104	1221:1235	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	6	67	theme	×	1231:1231	arg1	cfu/m3					1213:1218	1,745 cfu/m3	1207:1218	1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar	1207:1295	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	9	68	theme	Staphylococcus	1630:1643	arg1	species					1665:1671	different Staphylococcus and Corynebacterium species	1620:1671	different Staphylococcus and Corynebacterium species	1620:1671	The majority of bacterial species found were related to the human skin microflora, such as different Staphylococcus and Corynebacterium species.					
37300561	6	69	theme	cfu/m3	1305:1310	arg1	air					1312:1314	16 cfu/m3 air	1302:1314	16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257)	1302:1395	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	1	70	theme	negative	131:138	arg1	effects					147:153	negative health effects	131:153	negative health effects	131:153	Indoor microbial exposure may cause negative health effects.					
37300561	9	71	theme	Corynebacterium	1649:1663	arg1	species					1665:1671	different Staphylococcus and Corynebacterium species	1620:1671	different Staphylococcus and Corynebacterium species	1620:1671	The majority of bacterial species found were related to the human skin microflora, such as different Staphylococcus and Corynebacterium species.					
37300561	4	72	theme	members	645:651	arg1	groups					629:634	different groups	619:634	different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands	619:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	4	72	theme	members	645:651	arg1	members					645:651	staff members	639:651	staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands	639:845	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	6	73	theme	selective	1282:1290	arg1	agar					1292:1295	a Staphylococcus selective agar	1265:1295	a Staphylococcus selective agar	1265:1295	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	12	74	with	staff	2069:2073	arg1	care					2085:2088	more care	2080:2088	more care	2080:2088	In conclusion, we give an overview of the general microbial exposure in nursing homes and show that microbial exposures are higher for staff with more care and nursing tasks compared with administrative staff.					
37300561	12	74	with	staff	2069:2073	arg1	tasks					2102:2106	nursing tasks	2094:2106	nursing tasks	2094:2106	In conclusion, we give an overview of the general microbial exposure in nursing homes and show that microbial exposures are higher for staff with more care and nursing tasks compared with administrative staff.					
37300561	11	75	theme	resistant	1900:1908	arg1	isolate					1892:1898	one isolate resistant	1888:1908	one isolate resistant towards amphotericin B	1888:1931	Of 40 tested A. fumigatus isolates, we found one multiresistant isolate, which was resistant towards both itraconazole and voriconazole, and one isolate resistant towards amphotericin B.					
37300561	4	76	from	homes	558:562	arg1	Denmark					567:573	Denmark	567:573	Denmark	567:573	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	0	77	dep	species	86:92	arg1	Work					0:3	Work	0:3	Work in nursing homes and occupational exposure to endotoxin	0:59	Work in nursing homes and occupational exposure to endotoxin and bacterial and fungal species.					
37300561	2	78	from	exposure	210:217	arg1	homes					230:234	nursing homes	222:234	nursing homes	222:234	Only little is known about the occupational microbial exposure in nursing homes and the factors that influence the exposure.					
37300561	4	79	theme	work	745:748	arg1	tasks					750:754	various work tasks	737:754	various work tasks	737:754	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	6	80	theme	Staphylococcus	1267:1280	arg1	agar					1292:1295	a Staphylococcus selective agar	1265:1295	a Staphylococcus selective agar	1265:1295	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	6	81	theme	personal	1051:1058	arg1	samples					1069:1075	personal exposure samples	1051:1075	personal exposure samples	1051:1075	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	11	82	theme	tested	1753:1758	arg1	isolates					1773:1780	40 tested A. fumigatus isolates	1750:1780	40 tested A. fumigatus isolates	1750:1780	Of 40 tested A. fumigatus isolates, we found one multiresistant isolate, which was resistant towards both itraconazole and voriconazole, and one isolate resistant towards amphotericin B.					
37300561	4	83	theme	stationary	689:698	arg1	measurements					711:722	stationary bioaerosol measurements	689:722	stationary bioaerosol measurements taken during various work tasks	689:754	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	4	83	theme	stationary	689:698	arg1	day					684:686	a typical working day	666:686	a typical working day	666:686	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	12	84	from	overview	1960:1967	arg1	homes					2014:2018	nursing homes	2006:2018	nursing homes	2006:2018	In conclusion, we give an overview of the general microbial exposure in nursing homes and show that microbial exposures are higher for staff with more care and nursing tasks compared with administrative staff.					
37300561	3	85	theme	elderly	354:360	arg1	persons					362:368	elderly persons	354:368	elderly persons who may carry infectious or antimicrobial-resistant microorganisms	354:435	The exposure in nursing homes may be increased due to close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms and due to handling of laundry, such as used clothing and bed linen.					
37300561	3	86	theme	laundry	460:466	arg1	handling					448:455	handling	448:455	handling of laundry, such as used clothing and bed linen	448:503	The exposure in nursing homes may be increased due to close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms and due to handling of laundry, such as used clothing and bed linen.					
37300561	6	87	theme	pathogenic	1330:1339	arg1	fungi					1341:1345	potential pathogenic fungi	1320:1345	potential pathogenic fungi incubated at 37 °C (below detection limit to 257)	1320:1395	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	7	88	theme	making	1443:1448	arg1	bed					1439:1441	bed making	1439:1448	bed making	1439:1448	Bacterial exposures were elevated during bed making.					
37300561	2	89	from	factors	244:250	arg1	homes					230:234	nursing homes	222:234	nursing homes	222:234	Only little is known about the occupational microbial exposure in nursing homes and the factors that influence the exposure.					
37300561	8	90	theme	highest	1468:1474	arg1	concentrations					1486:1499	the highest bacterial concentrations	1464:1499	the highest bacterial concentrations	1464:1499	On surfaces, the highest bacterial concentrations were found on bed railings.					
37300561	12	91	theme	microbial	2034:2042	arg1	exposures					2044:2052	microbial exposures	2034:2052	microbial exposures	2034:2052	In conclusion, we give an overview of the general microbial exposure in nursing homes and show that microbial exposures are higher for staff with more care and nursing tasks compared with administrative staff.					
37300561	5	92	theme	Aspergillus	989:999	arg1	isolates					1011:1018	Aspergillus fumigatus isolates	989:1018	Aspergillus fumigatus isolates	989:1018	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	2	93	theme	microbial	200:208	arg1	exposure					210:217	the occupational microbial exposure	183:217	the occupational microbial exposure in nursing homes	183:234	Only little is known about the occupational microbial exposure in nursing homes and the factors that influence the exposure.					
37300561	4	94	theme	surface	796:802	arg1	swabs					804:808	environmental surface swabs	782:808	environmental surface swabs	782:808	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	5	95	from	concentrations	899:912	arg1	isolates					1011:1018	Aspergillus fumigatus isolates	989:1018	Aspergillus fumigatus isolates	989:1018	From the samples, we explored bacterial and fungal concentrations and species composition, endotoxin levels, and antimicrobial resistance in Aspergillus fumigatus isolates.					
37300561	6	96	theme	geometric	1109:1117	arg1	GM					1126:1127	GM	1126:1127	GM	1126:1127	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	6	96	theme	geometric	1109:1117	arg1	means					1119:1123	geometric means	1109:1123	geometric means (GM)	1109:1128	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
37300561	12	97	theme	nursing	2094:2100	arg1	tasks					2102:2106	nursing tasks	2094:2106	nursing tasks	2094:2106	In conclusion, we give an overview of the general microbial exposure in nursing homes and show that microbial exposures are higher for staff with more care and nursing tasks compared with administrative staff.					
37300561	3	98	from	exposure	285:292	arg1	homes					305:309	nursing homes	297:309	nursing homes	297:309	The exposure in nursing homes may be increased due to close contact with elderly persons who may carry infectious or antimicrobial-resistant microorganisms and due to handling of laundry, such as used clothing and bed linen.					
37300561	7	99	theme	Bacterial	1398:1406	arg1	exposures					1408:1416	Bacterial exposures	1398:1416	Bacterial exposures	1398:1416	Bacterial exposures were elevated during bed making.					
37300561	8	100	theme	bed	1515:1517	arg1	railings					1519:1526	bed railings	1515:1526	bed railings	1515:1526	On surfaces, the highest bacterial concentrations were found on bed railings.					
37300561	4	101	theme	dust	768:771	arg1	samples					773:779	dust samples	768:779	dust samples	768:779	We investigated the microbial exposure in 5 nursing homes in Denmark, by use of personal bioaerosol samples from different groups of staff members taken during a typical working day, stationary bioaerosol measurements taken during various work tasks, sedimented dust samples, environmental surface swabs, and swabs from staff members' hands.					
37300561	11	102	theme	A.	1760:1761	arg1	isolates					1773:1780	40 tested A. fumigatus isolates	1750:1780	40 tested A. fumigatus isolates	1750:1780	Of 40 tested A. fumigatus isolates, we found one multiresistant isolate, which was resistant towards both itraconazole and voriconazole, and one isolate resistant towards amphotericin B.					
37300561	6	103	theme	detection	1373:1381	arg1	limit					1383:1387	detection limit	1373:1387	detection limit to 257	1373:1394	Microbial concentrations from personal exposure samples differed among professions, and geometric means (GM) were 2,159 cfu/m3 (84 to 1.5 × 105) for bacteria incubated on nutrient agar, 1,745 cfu/m3 (82 to 2.0 × 104) for bacteria cultivated on a Staphylococcus selective agar, and 16 cfu/m3 air for potential pathogenic fungi incubated at 37 °C (below detection limit to 257).					
36863672	5	0	theme	good	1068:1071	arg1	anti-oxidant					1073:1084	good anti-oxidant	1068:1084	good anti-oxidant	1068:1084	Moreover, the oxidation of DA sufficiently scavenged free radicals and introduced the QMPD hydrogel with good anti-oxidant and anti-inflammatory abilities.					
36863672	7	1	theme	dressings	1352:1360	arg1	design					1328:1333	the design	1324:1333	the design of wound healing dressings	1324:1360	Therefore, the QMPD hydrogel is expected to provide a new method for the design of wound healing dressings.					
36863672	5	2	theme	QMPD	1049:1052	arg1	hydrogel					1054:1061	the QMPD hydrogel	1045:1061	the QMPD hydrogel	1045:1061	Moreover, the oxidation of DA sufficiently scavenged free radicals and introduced the QMPD hydrogel with good anti-oxidant and anti-inflammatory abilities.					
36863672	1	3	theme	ammonium	239:246	arg1	chitosan					248:255	methacrylate anhydride (MA) grafted quaternary ammonium chitosan	192:255	methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA)	192:264	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	1	3	theme	ammonium	239:246	arg1	QCS-MA					258:263	QCS-MA	258:263	QCS-MA	258:263	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	5	4	theme	anti-inflammatory	1090:1106	arg1	abilities					1108:1116	anti-inflammatory abilities	1090:1116	anti-inflammatory abilities	1090:1116	Moreover, the oxidation of DA sufficiently scavenged free radicals and introduced the QMPD hydrogel with good anti-oxidant and anti-inflammatory abilities.					
36863672	0	5	theme	wound	113:117	arg1	healing					119:125	wound healing	113:125	wound healing	113:125	Chitosan-based multifunctional hydrogel for sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	7	6	theme	new	1309:1311	arg1	method					1313:1318	a new method	1307:1318	a new method for the design of wound healing dressings	1307:1360	Therefore, the QMPD hydrogel is expected to provide a new method for the design of wound healing dressings.					
36863672	1	7	theme	wound	403:407	arg1	healing					409:415	wound healing	403:415	wound healing	403:415	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	4	8	from	bacteria	818:825	arg1	wounds					830:835	wounds	830:835	wounds	830:835	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	6	9	theme	tropical	1164:1171	arg1	structure					1173:1181	the extracellular matrix-mimic tropical structure	1133:1181	the extracellular matrix-mimic tropical structure	1133:1181	Together with the extracellular matrix-mimic tropical structure, the QMPD hydrogel significantly promoted the wound management of mice.					
36863672	2	10	theme	light-triggered	479:493	arg1	polymerization					495:508	the ultraviolet light-triggered polymerization	463:508	the ultraviolet light-triggered polymerization of QCS-MA	463:518	The QMPD hydrogel formation was initiated by the ultraviolet light-triggered polymerization of QCS-MA.					
36863672	4	11	theme	ammonium	729:736	arg1	capable					799:805	capable	799:805	capable	799:805	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	11	theme	ammonium	729:736	arg1	conversion					768:777	the photothermal conversion	751:777	the photothermal conversion of polydopamine	751:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	11	theme	ammonium	729:736	arg1	groups					708:713	the quaternary ammonium groups	684:713	the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine	684:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	11	theme	ammonium	729:736	arg1	ammonium					729:736	quaternary ammonium chitosan and the photothermal conversion of polydopamine	718:793	ammonium	729:736	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	1	12	theme	QMPD	165:168	arg1	hydrogel					155:162	a composite hydrogel	143:162	a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA)	143:311	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	1	12	theme	QMPD	165:168	arg1	hydrogel					170:177	QMPD hydrogel	165:177	QMPD hydrogel	165:177	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	0	13	theme	inflammation	61:72	arg1	elimination					74:84	sequential wound inflammation elimination	44:84	sequential wound inflammation elimination	44:84	Chitosan-based multifunctional hydrogel for sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	6	14	theme	mice	1249:1252	arg1	management					1235:1244	the wound management	1225:1244	the wound management of mice	1225:1252	Together with the extracellular matrix-mimic tropical structure, the QMPD hydrogel significantly promoted the wound management of mice.					
36863672	4	15	theme	quaternary	718:727	arg1	ammonium					729:736	quaternary ammonium chitosan and the photothermal conversion of polydopamine	718:793	ammonium	729:736	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	0	16	theme	multifunctional	15:29	arg1	hydrogel					31:38	Chitosan-based multifunctional hydrogel	0:38	Chitosan-based multifunctional hydrogel for sequential wound inflammation elimination, infection inhibition, and wound healing.	0:126	Chitosan-based multifunctional hydrogel for sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	4	17	from	capable	799:805	arg1	hydrogel					674:681	this hydrogel	669:681	this hydrogel	669:681	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	18	theme	bacteria	818:825	arg1	capable					799:805	capable	799:805	capable	799:805	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	18	theme	bacteria	818:825	arg1	conversion					768:777	the photothermal conversion	751:777	the photothermal conversion of polydopamine	751:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	18	theme	bacteria	818:825	arg1	groups					708:713	the quaternary ammonium groups	684:713	the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine	684:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	18	theme	bacteria	818:825	arg1	ammonium					729:736	quaternary ammonium chitosan and the photothermal conversion of polydopamine	718:793	ammonium	729:736	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	0	19	theme	Chitosan-based	0:13	arg1	hydrogel					31:38	Chitosan-based multifunctional hydrogel	0:38	Chitosan-based multifunctional hydrogel for sequential wound inflammation elimination, infection inhibition, and wound healing.	0:126	Chitosan-based multifunctional hydrogel for sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	4	20	theme	killing	810:816	arg1	bacteria					818:825	killing bacteria	810:825	killing bacteria on wounds	810:835	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	3	21	theme	electrostatic	550:562	arg1	interactions					564:575	electrostatic interactions	550:575	electrostatic interactions	550:575	Furthermore, hydrogen bonds, electrostatic interactions, and "π-π" stacking between QCS-MA, PVP, and DA were involved in the hydrogel formation.					
36863672	4	22	theme	photothermal	755:766	arg1	conversion					768:777	the photothermal conversion	751:777	the photothermal conversion of polydopamine	751:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	7	23	theme	healing	1344:1350	arg1	dressings					1352:1360	wound healing dressings	1338:1360	wound healing dressings	1338:1360	Therefore, the QMPD hydrogel is expected to provide a new method for the design of wound healing dressings.					
36863672	3	24	theme	hydrogen	534:541	arg1	bonds					543:547	hydrogen bonds	534:547	hydrogen bonds	534:547	Furthermore, hydrogen bonds, electrostatic interactions, and "π-π" stacking between QCS-MA, PVP, and DA were involved in the hydrogel formation.					
36863672	6	25	theme	wound	1229:1233	arg1	management					1235:1244	the wound management	1225:1244	the wound management of mice	1225:1252	Together with the extracellular matrix-mimic tropical structure, the QMPD hydrogel significantly promoted the wound management of mice.					
36863672	6	26	theme	matrix-mimic	1151:1162	arg1	structure					1173:1181	the extracellular matrix-mimic tropical structure	1133:1181	the extracellular matrix-mimic tropical structure	1133:1181	Together with the extracellular matrix-mimic tropical structure, the QMPD hydrogel significantly promoted the wound management of mice.					
36863672	1	27	theme	sequential	334:343	arg1	elimination					364:374	the sequential wound inflammation elimination	330:374	the sequential wound inflammation elimination	330:374	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	4	28	theme	quaternary	688:697	arg1	capable					799:805	capable	799:805	capable	799:805	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	28	theme	quaternary	688:697	arg1	conversion					768:777	the photothermal conversion	751:777	the photothermal conversion of polydopamine	751:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	28	theme	quaternary	688:697	arg1	groups					708:713	the quaternary ammonium groups	684:713	the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine	684:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	28	theme	quaternary	688:697	arg1	ammonium					729:736	quaternary ammonium chitosan and the photothermal conversion of polydopamine	718:793	ammonium	729:736	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	1	29	theme	methacrylate	192:203	arg1	chitosan					248:255	methacrylate anhydride (MA) grafted quaternary ammonium chitosan	192:255	methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA)	192:264	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	1	29	theme	methacrylate	192:203	arg1	QCS-MA					258:263	QCS-MA	258:263	QCS-MA	258:263	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	0	30	theme	wound	55:59	arg1	elimination					74:84	sequential wound inflammation elimination	44:84	sequential wound inflammation elimination	44:84	Chitosan-based multifunctional hydrogel for sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	1	31	theme	wound	345:349	arg1	elimination					364:374	the sequential wound inflammation elimination	330:374	the sequential wound inflammation elimination	330:374	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	4	32	theme	%	885:885	arg1	ratios					870:875	the bacteriostatic ratios	851:875	the bacteriostatic ratios of 85.6 % and 92.5 %	851:896	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	1	33	theme	anhydride	205:213	arg1	chitosan					248:255	methacrylate anhydride (MA) grafted quaternary ammonium chitosan	192:255	methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA)	192:264	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	1	33	theme	anhydride	205:213	arg1	QCS-MA					258:263	QCS-MA	258:263	QCS-MA	258:263	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	0	34	theme	sequential	44:53	arg1	elimination					74:84	sequential wound inflammation elimination	44:84	sequential wound inflammation elimination	44:84	Chitosan-based multifunctional hydrogel for sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	2	35	theme	hydrogel	427:434	arg1	formation					436:444	The QMPD hydrogel formation	418:444	The QMPD hydrogel formation	418:444	The QMPD hydrogel formation was initiated by the ultraviolet light-triggered polymerization of QCS-MA.					
36863672	1	36	theme	inflammation	351:362	arg1	elimination					364:374	the sequential wound inflammation elimination	330:374	the sequential wound inflammation elimination	330:374	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	3	37	theme	"	582:582	arg1	stacking					588:595	"π-π" stacking	582:595	"π-π" stacking	582:595	Furthermore, hydrogen bonds, electrostatic interactions, and "π-π" stacking between QCS-MA, PVP, and DA were involved in the hydrogel formation.					
36863672	2	38	theme	ultraviolet	467:477	arg1	polymerization					495:508	the ultraviolet light-triggered polymerization	463:508	the ultraviolet light-triggered polymerization of QCS-MA	463:518	The QMPD hydrogel formation was initiated by the ultraviolet light-triggered polymerization of QCS-MA.					
36863672	2	39	theme	QMPD	422:425	arg1	formation					436:444	The QMPD hydrogel formation	418:444	The QMPD hydrogel formation	418:444	The QMPD hydrogel formation was initiated by the ultraviolet light-triggered polymerization of QCS-MA.					
36863672	4	40	theme	%	896:896	arg1	ratios					870:875	the bacteriostatic ratios	851:875	the bacteriostatic ratios of 85.6 % and 92.5 %	851:896	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	7	41	theme	wound	1338:1342	arg1	dressings					1352:1360	wound healing dressings	1338:1360	wound healing dressings	1338:1360	Therefore, the QMPD hydrogel is expected to provide a new method for the design of wound healing dressings.					
36863672	2	42	theme	QCS-MA	513:518	arg1	polymerization					495:508	the ultraviolet light-triggered polymerization	463:508	the ultraviolet light-triggered polymerization of QCS-MA	463:518	The QMPD hydrogel formation was initiated by the ultraviolet light-triggered polymerization of QCS-MA.					
36863672	5	43	theme	DA	990:991	arg1	oxidation					977:985	the oxidation	973:985	the oxidation of DA	973:991	Moreover, the oxidation of DA sufficiently scavenged free radicals and introduced the QMPD hydrogel with good anti-oxidant and anti-inflammatory abilities.					
36863672	4	44	theme	polydopamine	782:793	arg1	conversion					768:777	the photothermal conversion	751:777	the photothermal conversion of polydopamine	751:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	44	theme	polydopamine	782:793	arg1	ammonium					729:736	quaternary ammonium chitosan and the photothermal conversion of polydopamine	718:793	ammonium	729:736	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	7	45	theme	QMPD	1270:1273	arg1	hydrogel					1275:1282	the QMPD hydrogel	1266:1282	the QMPD hydrogel	1266:1282	Therefore, the QMPD hydrogel is expected to provide a new method for the design of wound healing dressings.					
36863672	4	46	from	hydrogel	674:681	arg1	capable					799:805	capable	799:805	capable	799:805	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	46	from	hydrogel	674:681	arg1	conversion					768:777	the photothermal conversion	751:777	the photothermal conversion of polydopamine	751:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	46	from	hydrogel	674:681	arg1	groups					708:713	the quaternary ammonium groups	684:713	the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine	684:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	46	from	hydrogel	674:681	arg1	ammonium					729:736	quaternary ammonium chitosan and the photothermal conversion of polydopamine	718:793	ammonium	729:736	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	1	47	theme	infection	377:385	arg1	inhibition					387:396	infection inhibition	377:396	infection inhibition	377:396	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	4	48	theme	conversion	768:777	arg1	capable					799:805	capable	799:805	capable	799:805	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	48	theme	conversion	768:777	arg1	conversion					768:777	the photothermal conversion	751:777	the photothermal conversion of polydopamine	751:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	48	theme	conversion	768:777	arg1	groups					708:713	the quaternary ammonium groups	684:713	the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine	684:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	48	theme	conversion	768:777	arg1	ammonium					729:736	quaternary ammonium chitosan and the photothermal conversion of polydopamine	718:793	ammonium	729:736	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	49	theme	ammonium	699:706	arg1	capable					799:805	capable	799:805	capable	799:805	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	49	theme	ammonium	699:706	arg1	conversion					768:777	the photothermal conversion	751:777	the photothermal conversion of polydopamine	751:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	49	theme	ammonium	699:706	arg1	groups					708:713	the quaternary ammonium groups	684:713	the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine	684:793	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	49	theme	ammonium	699:706	arg1	ammonium					729:736	quaternary ammonium chitosan and the photothermal conversion of polydopamine	718:793	ammonium	729:736	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	4	50	theme	bacteriostatic	855:868	arg1	ratios					870:875	the bacteriostatic ratios	851:875	the bacteriostatic ratios of 85.6 % and 92.5 %	851:896	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	6	51	theme	QMPD	1188:1191	arg1	hydrogel					1193:1200	the QMPD hydrogel	1184:1200	the QMPD hydrogel	1184:1200	Together with the extracellular matrix-mimic tropical structure, the QMPD hydrogel significantly promoted the wound management of mice.					
36863672	0	52	theme	infection	87:95	arg1	inhibition					97:106	infection inhibition	87:106	infection inhibition	87:106	Chitosan-based multifunctional hydrogel for sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	5	53	theme	free	1016:1019	arg1	radicals					1021:1028	free radicals	1016:1028	free radicals	1016:1028	Moreover, the oxidation of DA sufficiently scavenged free radicals and introduced the QMPD hydrogel with good anti-oxidant and anti-inflammatory abilities.					
36863672	3	54	theme	π-π	583:585	arg1	stacking					588:595	"π-π" stacking	582:595	"π-π" stacking	582:595	Furthermore, hydrogen bonds, electrostatic interactions, and "π-π" stacking between QCS-MA, PVP, and DA were involved in the hydrogel formation.					
36863672	6	55	theme	extracellular	1137:1149	arg1	structure					1173:1181	the extracellular matrix-mimic tropical structure	1133:1181	the extracellular matrix-mimic tropical structure	1133:1181	Together with the extracellular matrix-mimic tropical structure, the QMPD hydrogel significantly promoted the wound management of mice.					
36863672	4	56	dep	ammonium	729:736	arg1	chitosan					738:745	chitosan	738:745	chitosan	738:745	In this hydrogel, the quaternary ammonium groups of quaternary ammonium chitosan and the photothermal conversion of polydopamine are capable of killing bacteria on wounds, which showed the bacteriostatic ratios of 85.6 % and 92.5 % toward Escherichia coli and Staphylococcus aureus, respectively.					
36863672	1	57	theme	grafted	220:226	arg1	chitosan					248:255	methacrylate anhydride (MA) grafted quaternary ammonium chitosan	192:255	methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA)	192:264	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	1	57	theme	grafted	220:226	arg1	QCS-MA					258:263	QCS-MA	258:263	QCS-MA	258:263	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	3	58	theme	hydrogel	646:653	arg1	formation					655:663	the hydrogel formation	642:663	the hydrogel formation	642:663	Furthermore, hydrogen bonds, electrostatic interactions, and "π-π" stacking between QCS-MA, PVP, and DA were involved in the hydrogel formation.					
36863672	3	59	theme	"	586:586	arg1	stacking					588:595	"π-π" stacking	582:595	"π-π" stacking	582:595	Furthermore, hydrogen bonds, electrostatic interactions, and "π-π" stacking between QCS-MA, PVP, and DA were involved in the hydrogel formation.					
36863672	1	60	theme	composite	145:153	arg1	hydrogel					155:162	a composite hydrogel	143:162	a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA)	143:311	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	1	60	theme	composite	145:153	arg1	hydrogel					170:177	QMPD hydrogel	165:177	QMPD hydrogel	165:177	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	1	61	theme	quaternary	228:237	arg1	chitosan					248:255	methacrylate anhydride (MA) grafted quaternary ammonium chitosan	192:255	methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA)	192:264	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
36863672	1	61	theme	quaternary	228:237	arg1	QCS-MA					258:263	QCS-MA	258:263	QCS-MA	258:263	In this study, a composite hydrogel (QMPD hydrogel) composed of methacrylate anhydride (MA) grafted quaternary ammonium chitosan (QCS-MA), polyvinylpyrrolidone (PVP), and dopamine (DA) was designed for the sequential wound inflammation elimination, infection inhibition, and wound healing.					
35400500	0	0	theme	gut	80:82	arg1	Correlations					0:11	Correlations	0:11	Correlations between oligosaccharides in breast milk	0:51	Correlations between oligosaccharides in breast milk and the composition of the gut microbiome in breastfed infants.					
35400500	0	0	theme	gut	80:82	arg1	composition					61:71	the composition	57:71	the composition of the gut	57:82	Correlations between oligosaccharides in breast milk and the composition of the gut microbiome in breastfed infants.					
35400500	0	1	from	oligosaccharides	21:36	arg1	milk					48:51	breast milk	41:51	breast milk	41:51	Correlations between oligosaccharides in breast milk and the composition of the gut microbiome in breastfed infants.					
35400500	7	2	theme	milk-derived	1476:1487	arg1	HMO					1489:1491	breast milk-derived HMO	1469:1491	breast milk-derived HMO	1469:1491	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	1	3	from	stages	164:169	arg1	composition					121:131	The composition	117:131	The composition of the microbiome in the early stages of life	117:177	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	7	4	theme	breast	1469:1474	arg1	HMO					1489:1491	breast milk-derived HMO	1469:1491	breast milk-derived HMO	1469:1491	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	1	5	theme	critical	304:311	arg1	regulators					313:322	critical regulators	304:322	critical regulators in the maintenance of a healthy gut microbiota in infants	304:380	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	1	5	theme	critical	304:311	arg1	oligosaccharides					277:292	human milk oligosaccharides	266:292	human milk oligosaccharides (HMO)	266:298	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	3	6	theme	liquid	624:629	arg1	spectrometry					651:662	liquid chromatography-mass spectrometry	624:662	liquid chromatography-mass spectrometry	624:662	Quantification of HMO was conducted via liquid chromatography-mass spectrometry, and bacterial DNA sequencing was employed for characterization of the fecal microbiota.					
35400500	2	7	theme	profiles	539:546	arg1	Jining					473:478	Jining	473:478	Jining	473:478	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	7	theme	profiles	539:546	arg1	analysis					519:526	a corresponding analysis	503:526	a corresponding analysis of the HMO profiles in samples of maternal breast milk	503:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	7	theme	profiles	539:546	arg1	China					492:496	China	492:496	China	492:496	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	7	theme	profiles	539:546	arg1	Harbin					484:489	Harbin	484:489	Harbin	484:489	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	8	theme	1-mo-old	441:448	arg1	infants					460:466	1-mo-old breastfed infants	441:466	1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk	441:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	7	9	theme	microbiota	1534:1543	arg1	shaping					1515:1521	the shaping	1511:1521	the shaping of the gut microbiota in breastfed infants	1511:1564	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	4	10	from	group	934:938	arg1	samples					910:916	samples	910:916	samples from the Jining group relative to the Harbin group	910:967	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	3	11	theme	chromatography-mass	631:649	arg1	spectrometry					651:662	liquid chromatography-mass spectrometry	624:662	liquid chromatography-mass spectrometry	624:662	Quantification of HMO was conducted via liquid chromatography-mass spectrometry, and bacterial DNA sequencing was employed for characterization of the fecal microbiota.					
35400500	2	12	theme	HMO	535:537	arg1	profiles					539:546	the HMO profiles	531:546	the HMO profiles in samples of maternal breast milk	531:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	7	13	theme	gut	1448:1450	arg1	microflora					1452:1461	the gut microflora	1444:1461	the gut microflora	1444:1461	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	1	14	theme	prior	237:241	arg1	evidence					243:250	prior evidence	237:250	prior evidence	237:250	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	4	15	theme	relative	940:947	arg1	group					934:938	the Jining group	923:938	the Jining group relative to the Harbin group	923:967	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	3	16	theme	DNA	679:681	arg1	sequencing					683:692	bacterial DNA sequencing	669:692	bacterial DNA sequencing	669:692	Quantification of HMO was conducted via liquid chromatography-mass spectrometry, and bacterial DNA sequencing was employed for characterization of the fecal microbiota.					
35400500	6	17	theme	young	1270:1274	arg1	infants					1276:1282	young infants	1270:1282	young infants	1270:1282	Correlation analyses evaluating microbes and 19 different HMO indicated that HMO were beneficial to the development of the gut microbiota in young infants.					
35400500	3	18	theme	bacterial	669:677	arg1	sequencing					683:692	bacterial DNA sequencing	669:692	bacterial DNA sequencing	669:692	Quantification of HMO was conducted via liquid chromatography-mass spectrometry, and bacterial DNA sequencing was employed for characterization of the fecal microbiota.					
35400500	2	19	from	analysis	519:526	arg1	samples					551:557	samples	551:557	samples of maternal breast milk	551:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	4	20	theme	Harbin	956:961	arg1	group					963:967	the Harbin group	952:967	the Harbin group	952:967	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	6	21	theme	microbiota	1256:1265	arg1	development					1233:1243	the development	1229:1243	the development of the gut microbiota in young infants	1229:1282	Correlation analyses evaluating microbes and 19 different HMO indicated that HMO were beneficial to the development of the gut microbiota in young infants.					
35400500	2	22	theme	breast	571:576	arg1	milk					578:581	maternal breast milk	562:581	maternal breast milk	562:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	1	23	from	microbiome	140:149	arg1	stages					164:169	the early stages	154:169	the early stages of life	154:177	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	6	24	theme	different	1177:1185	arg1	HMO					1187:1189	19 different HMO	1174:1189	19 different HMO	1174:1189	Correlation analyses evaluating microbes and 19 different HMO indicated that HMO were beneficial to the development of the gut microbiota in young infants.					
35400500	5	25	from	species	1017:1023	arg1	Jining					1057:1062	Jining	1057:1062	Jining	1057:1062	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	5	25	from	species	1017:1023	arg1	infants					1028:1034	infants	1028:1034	infants from both Harbin and Jining	1028:1062	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	5	25	from	species	1017:1023	arg1	Harbin					1046:1051	Harbin	1046:1051	Harbin	1046:1051	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	1	26	theme	early	158:162	arg1	stages					164:169	the early stages	154:169	the early stages of life	154:177	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	0	27	theme	breastfed	98:106	arg1	infants					108:114	breastfed infants	98:114	breastfed infants	98:114	Correlations between oligosaccharides in breast milk and the composition of the gut microbiome in breastfed infants.					
35400500	2	28	from	microbiota	427:436	arg1	Jining					473:478	Jining	473:478	Jining	473:478	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	28	from	microbiota	427:436	arg1	Harbin					484:489	Harbin	484:489	Harbin	484:489	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	29	theme	microbiota	427:436	arg1	analysis					407:414	an analysis	404:414	an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk	404:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	1	30	from	composition	121:131	arg1	stages					164:169	the early stages	154:169	the early stages of life	154:177	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	5	31	from	Jining	1057:1062	arg1	infants					1028:1034	infants	1028:1034	infants from both Harbin and Jining	1028:1062	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	5	31	from	Jining	1057:1062	arg1	species					1017:1023	the predominant microbial species	991:1023	the predominant microbial species in infants from both Harbin and Jining	991:1062	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	5	31	from	Jining	1057:1062	arg1	Bifidobacterium					970:984	Bifidobacterium	970:984	Bifidobacterium	970:984	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	2	32	from	profiles	539:546	arg1	samples					551:557	samples	551:557	samples of maternal breast milk	551:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	6	33	theme	gut	1252:1254	arg1	microbiota					1256:1265	the gut microbiota	1248:1265	the gut microbiota	1248:1265	Correlation analyses evaluating microbes and 19 different HMO indicated that HMO were beneficial to the development of the gut microbiota in young infants.					
35400500	3	34	theme	HMO	602:604	arg1	Quantification					584:597	Quantification	584:597	Quantification of HMO	584:604	Quantification of HMO was conducted via liquid chromatography-mass spectrometry, and bacterial DNA sequencing was employed for characterization of the fecal microbiota.					
35400500	7	35	link	milk-derived	1476:1487	arg1	HMO					1489:1491	breast milk-derived HMO	1469:1491	breast milk-derived HMO	1469:1491	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	4	36	theme	lacto-N-fucopentaose	824:843	arg1	I					845:845	lacto-N-fucopentaose I	824:845	lacto-N-fucopentaose I	824:845	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	4	36	theme	lacto-N-fucopentaose	824:843	arg1	oligosaccharides					785:800	total neutral oligosaccharides	771:800	total neutral oligosaccharides	771:800	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	3	37	theme	microbiota	741:750	arg1	characterization					711:726	characterization	711:726	characterization of the fecal microbiota	711:750	Quantification of HMO was conducted via liquid chromatography-mass spectrometry, and bacterial DNA sequencing was employed for characterization of the fecal microbiota.					
35400500	1	38	theme	life	174:177	arg1	stages					164:169	the early stages	154:169	the early stages of life	154:177	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	7	39	from	infants	1558:1564	arg1	shaping					1515:1521	the shaping	1511:1521	the shaping of the gut microbiota in breastfed infants	1511:1564	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	2	40	theme	milk	578:581	arg1	samples					551:557	samples	551:557	samples of maternal breast milk	551:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	7	41	theme	Bifidobacterium	1305:1319	arg1	predominance					1289:1300	The predominance	1285:1300	The predominance of Bifidobacterium in these microbial communities	1285:1350	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	1	42	from	maintenance	331:341	arg1	infants					374:380	infants	374:380	infants	374:380	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	1	43	theme	human	266:270	arg1	regulators					313:322	critical regulators	304:322	critical regulators in the maintenance of a healthy gut microbiota in infants	304:380	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	1	43	theme	human	266:270	arg1	HMO					295:297	HMO	295:297	HMO	295:297	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	1	43	theme	human	266:270	arg1	oligosaccharides					277:292	human milk oligosaccharides	266:292	human milk oligosaccharides (HMO)	266:298	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	1	44	theme	healthy	348:354	arg1	microbiota					360:369	a healthy gut microbiota	346:369	a healthy gut microbiota	346:369	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	1	45	from	regulators	313:322	arg1	maintenance					331:341	the maintenance	327:341	the maintenance of a healthy gut microbiota in infants	327:380	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	1	46	theme	milk	272:275	arg1	regulators					313:322	critical regulators	304:322	critical regulators in the maintenance of a healthy gut microbiota in infants	304:380	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	1	46	theme	milk	272:275	arg1	HMO					295:297	HMO	295:297	HMO	295:297	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	1	46	theme	milk	272:275	arg1	oligosaccharides					277:292	human milk oligosaccharides	266:292	human milk oligosaccharides (HMO)	266:298	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	1	47	theme	gut	356:358	arg1	microbiota					360:369	a healthy gut microbiota	346:369	a healthy gut microbiota	346:369	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	4	48	theme	neutral	777:783	arg1	disialyl-lacto-N-tetraose					852:876	disialyl-lacto-N-tetraose	852:876	disialyl-lacto-N-tetraose	852:876	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	4	48	theme	neutral	777:783	arg1	lactodifucotetraose					803:821	lactodifucotetraose	803:821	lactodifucotetraose	803:821	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	4	48	theme	neutral	777:783	arg1	I					845:845	lacto-N-fucopentaose I	824:845	lacto-N-fucopentaose I	824:845	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	4	48	theme	neutral	777:783	arg1	oligosaccharides					785:800	total neutral oligosaccharides	771:800	total neutral oligosaccharides	771:800	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	7	49	theme	microbial	1330:1338	arg1	communities					1340:1350	these microbial communities	1324:1350	these microbial communities	1324:1350	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	7	50	theme	microflora	1452:1461	arg1	homeostasis					1429:1439	the homeostasis	1425:1439	the homeostasis of the gut microflora	1425:1461	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	0	51	theme	breast	41:46	arg1	milk					48:51	breast milk	41:51	breast milk	41:51	Correlations between oligosaccharides in breast milk and the composition of the gut microbiome in breastfed infants.					
35400500	3	52	theme	fecal	735:739	arg1	microbiota					741:750	the fecal microbiota	731:750	the fecal microbiota	731:750	Quantification of HMO was conducted via liquid chromatography-mass spectrometry, and bacterial DNA sequencing was employed for characterization of the fecal microbiota.					
35400500	2	53	theme	gut	423:425	arg1	microbiota					427:436	the gut microbiota	419:436	the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk	419:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	1	54	theme	microbiota	360:369	arg1	maintenance					331:341	the maintenance	327:341	the maintenance of a healthy gut microbiota in infants	327:380	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	4	55	theme	total	771:775	arg1	disialyl-lacto-N-tetraose					852:876	disialyl-lacto-N-tetraose	852:876	disialyl-lacto-N-tetraose	852:876	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	4	55	theme	total	771:775	arg1	lactodifucotetraose					803:821	lactodifucotetraose	803:821	lactodifucotetraose	803:821	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	4	55	theme	total	771:775	arg1	I					845:845	lacto-N-fucopentaose I	824:845	lacto-N-fucopentaose I	824:845	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	4	55	theme	total	771:775	arg1	oligosaccharides					785:800	total neutral oligosaccharides	771:800	total neutral oligosaccharides	771:800	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	7	56	from	predominance	1289:1300	arg1	communities					1340:1350	these microbial communities	1324:1350	these microbial communities	1324:1350	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	2	57	from	samples	551:557	arg1	Jining					473:478	Jining	473:478	Jining	473:478	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	57	from	samples	551:557	arg1	analysis					519:526	a corresponding analysis	503:526	a corresponding analysis of the HMO profiles in samples of maternal breast milk	503:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	57	from	samples	551:557	arg1	China					492:496	China	492:496	China	492:496	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	57	from	samples	551:557	arg1	Harbin					484:489	Harbin	484:489	Harbin	484:489	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	4	58	theme	oligosaccharides	785:800	arg1	abundances					757:766	The abundances	753:766	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose	753:876	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	7	59	attach	predominance	1289:1300	arg1	communities					1340:1350	these microbial communities	1324:1350	these microbial communities	1324:1350	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	7	59	attach	predominance	1289:1300	arg2	Bifidobacterium					1305:1319	Bifidobacterium	1305:1319	Bifidobacterium	1305:1319	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	6	60	theme	Correlation	1129:1139	arg1	analyses					1141:1148	Correlation analyses	1129:1148	Correlation analyses evaluating microbes and 19 different HMO	1129:1189	Correlation analyses evaluating microbes and 19 different HMO indicated that HMO were beneficial to the development of the gut microbiota in young infants.					
35400500	4	61	theme	Jining	927:932	arg1	group					934:938	the Jining group	923:938	the Jining group relative to the Harbin group	923:967	The abundances of total neutral oligosaccharides, lactodifucotetraose, lacto-N-fucopentaose I, and disialyl-lacto-N-tetraose were significantly increased in samples from the Jining group relative to the Harbin group.					
35400500	7	62	from	microbiota	1534:1543	arg1	infants					1558:1564	breastfed infants	1548:1564	breastfed infants	1548:1564	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	2	63	from	Jining	473:478	arg1	infants					460:466	1-mo-old breastfed infants	441:466	1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk	441:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	63	from	Jining	473:478	arg1	samples					551:557	samples	551:557	samples of maternal breast milk	551:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	63	from	Jining	473:478	arg1	microbiota					427:436	the gut microbiota	419:436	the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk	419:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	64	theme	maternal	562:569	arg1	milk					578:581	maternal breast milk	562:581	maternal breast milk	562:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	7	65	theme	gut	1530:1532	arg1	microbiota					1534:1543	the gut microbiota	1526:1543	the gut microbiota in breastfed infants	1526:1564	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	7	66	theme	breastfed	1548:1556	arg1	infants					1558:1564	breastfed infants	1548:1564	breastfed infants	1548:1564	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	2	67	theme	corresponding	505:517	arg1	analysis					519:526	a corresponding analysis	503:526	a corresponding analysis of the HMO profiles in samples of maternal breast milk	503:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	7	68	from	shaping	1515:1521	arg1	infants					1558:1564	breastfed infants	1548:1564	breastfed infants	1548:1564	The predominance of Bifidobacterium in these microbial communities suggests that their ability to efficiently utilize HMO can contribute to the homeostasis of the gut microflora, with breast milk-derived HMO being critical to the shaping of the gut microbiota in breastfed infants.					
35400500	2	69	from	China	492:496	arg1	samples					551:557	samples	551:557	samples of maternal breast milk	551:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	70	from	Harbin	484:489	arg1	infants					460:466	1-mo-old breastfed infants	441:466	1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk	441:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	70	from	Harbin	484:489	arg1	samples					551:557	samples	551:557	samples of maternal breast milk	551:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	2	70	from	Harbin	484:489	arg1	microbiota					427:436	the gut microbiota	419:436	the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk	419:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	5	71	theme	predominant	995:1005	arg1	species					1017:1023	the predominant microbial species	991:1023	the predominant microbial species in infants from both Harbin and Jining	991:1062	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	5	71	theme	predominant	995:1005	arg1	Bifidobacterium					970:984	Bifidobacterium	970:984	Bifidobacterium	970:984	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	2	72	theme	infants	460:466	arg1	microbiota					427:436	the gut microbiota	419:436	the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk	419:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	5	73	theme	microbial	1007:1015	arg1	species					1017:1023	the predominant microbial species	991:1023	the predominant microbial species in infants from both Harbin and Jining	991:1062	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	5	73	theme	microbial	1007:1015	arg1	Bifidobacterium					970:984	Bifidobacterium	970:984	Bifidobacterium	970:984	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	6	74	from	development	1233:1243	arg1	infants					1276:1282	young infants	1270:1282	young infants	1270:1282	Correlation analyses evaluating microbes and 19 different HMO indicated that HMO were beneficial to the development of the gut microbiota in young infants.					
35400500	1	75	theme	developing	213:222	arg1	infants					224:230	developing infants	213:230	developing infants	213:230	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	2	76	theme	breastfed	450:458	arg1	infants					460:466	1-mo-old breastfed infants	441:466	1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk	441:581	Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk.					
35400500	5	77	theme	former	1117:1122	arg1	set					1124:1126	the former set	1113:1126	the former set	1113:1126	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	5	78	from	Harbin	1046:1051	arg1	infants					1028:1034	infants	1028:1034	infants from both Harbin and Jining	1028:1062	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	5	78	from	Harbin	1046:1051	arg1	species					1017:1023	the predominant microbial species	991:1023	the predominant microbial species in infants from both Harbin and Jining	991:1062	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	5	78	from	Harbin	1046:1051	arg1	Bifidobacterium					970:984	Bifidobacterium	970:984	Bifidobacterium	970:984	Bifidobacterium were the predominant microbial species in infants from both Harbin and Jining, with these levels being significantly higher in the former set.					
35400500	1	79	theme	microbiome	140:149	arg1	composition					121:131	The composition	117:131	The composition of the microbiome in the early stages of life	117:177	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
35400500	1	80	theme	infants	224:230	arg1	health					203:208	the health	199:208	the health of developing infants	199:230	The composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants.					
36811411	0	0	theme	anatomical	54:63	arg1	studies					65:71	anatomical studies	54:71	anatomical studies of woody plants	54:87	Laser ablation tomography (LATscan) as a new tool for anatomical studies of woody plants.					
36811411	6	1	theme	tissue	988:993	arg1	composition					995:1005	tissue composition	988:1005	tissue composition	988:1005	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	3	2	theme	Laser	469:473	arg1	system					534:539	a high-throughput imaging system	508:539	a high-throughput imaging system that yields hundreds of images per minute	508:581	Laser Ablation Tomography (LATscan) is a high-throughput imaging system that yields hundreds of images per minute.					
36811411	3	2	theme	Laser	469:473	arg1	LATscan					496:502	LATscan	496:502	LATscan	496:502	Laser Ablation Tomography (LATscan) is a high-throughput imaging system that yields hundreds of images per minute.					
36811411	3	2	theme	Laser	469:473	arg1	Tomography					484:493	Laser Ablation Tomography	469:493	Laser Ablation Tomography (LATscan)	469:503	Laser Ablation Tomography (LATscan) is a high-throughput imaging system that yields hundreds of images per minute.					
36811411	8	3	contain	has	1435:1437	arg1	technology					1424:1433	This high-throughput imaging technology	1395:1433	This high-throughput imaging technology	1395:1433	This high-throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.					
36811411	8	3	contain	has	1435:1437	arg2	potential					1443:1451	the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems	1439:1557	the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems	1439:1557	This high-throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.					
36811411	8	4	theme	high-throughput	1400:1414	arg1	technology					1424:1433	This high-throughput imaging technology	1395:1433	This high-throughput imaging technology	1395:1433	This high-throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.					
36811411	2	5	with	vines	373:377	arg1	anatomies					407:415	heterogeneous anatomies	393:415	heterogeneous anatomies	393:415	This approach generates significant details; however, this workflow is laborious, particularly in woody vines (lianas) with heterogeneous anatomies, and ultimately yields two-dimensional (2D) images.					
36811411	5	6	theme	species	846:852	arg1	lianas					818:823	lianas	818:823	lianas (ca. 20 mm) of seven species	818:852	We report LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species and compare these results to those obtained through traditional anatomical techniques.					
36811411	5	6	theme	species	846:852	arg1	20 mm					830:834	20 mm	830:834	20 mm	830:834	We report LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species and compare these results to those obtained through traditional anatomical techniques.					
36811411	3	7	theme	Ablation	475:482	arg1	system					534:539	a high-throughput imaging system	508:539	a high-throughput imaging system that yields hundreds of images per minute	508:581	Laser Ablation Tomography (LATscan) is a high-throughput imaging system that yields hundreds of images per minute.					
36811411	3	7	theme	Ablation	475:482	arg1	LATscan					496:502	LATscan	496:502	LATscan	496:502	Laser Ablation Tomography (LATscan) is a high-throughput imaging system that yields hundreds of images per minute.					
36811411	3	7	theme	Ablation	475:482	arg1	Tomography					484:493	Laser Ablation Tomography	469:493	Laser Ablation Tomography (LATscan)	469:503	Laser Ablation Tomography (LATscan) is a high-throughput imaging system that yields hundreds of images per minute.					
36811411	8	8	theme	vegetative	1479:1488	arg1	anatomy					1507:1513	vegetative and reproductive anatomy	1479:1513	vegetative and reproductive anatomy	1479:1513	This high-throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.					
36811411	4	9	theme	woody	722:726	arg1	tissues					728:734	woody tissues	722:734	woody tissues	722:734	This method has proven useful for studying the structure of delicate plant tissues, however its utility in understanding the structure of woody tissues is underexplored.					
36811411	5	10	theme	traditional	906:916	arg1	techniques					929:938	traditional anatomical techniques	906:938	traditional anatomical techniques	906:938	We report LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species and compare these results to those obtained through traditional anatomical techniques.					
36811411	7	11	theme	qualitative	1356:1366	arg1	analyses					1385:1392	both qualitative and quantitative analyses	1351:1392	both qualitative and quantitative analyses	1351:1392	LATscan generate high-quality 2D images and 3D reconstructions of woody plant samples, therefore this new technology is useful for both qualitative and quantitative analyses.					
36811411	8	12	theme	anatomy	1507:1513	arg1	phenotyping					1464:1474	phenotyping	1464:1474	phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems	1464:1557	This high-throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.					
36811411	2	13	dep	two-dimensional	440:454	arg1	2D					457:458	2D	457:458	2D	457:458	This approach generates significant details; however, this workflow is laborious, particularly in woody vines (lianas) with heterogeneous anatomies, and ultimately yields two-dimensional (2D) images.					
36811411	4	14	theme	tissues	659:665	arg1	structure					631:639	the structure	627:639	the structure of delicate plant tissues, however its utility in understanding the structure of woody tissues is underexplored	627:751	This method has proven useful for studying the structure of delicate plant tissues, however its utility in understanding the structure of woody tissues is underexplored.					
36811411	0	15	theme	plants	82:87	arg1	studies					65:71	anatomical studies	54:71	anatomical studies of woody plants	54:87	Laser ablation tomography (LATscan) as a new tool for anatomical studies of woody plants.					
36811411	8	16	theme	reproductive	1494:1505	arg1	anatomy					1507:1513	vegetative and reproductive anatomy	1479:1513	vegetative and reproductive anatomy	1479:1513	This high-throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.					
36811411	5	17	theme	LATscan-derived	764:778	arg1	data					791:794	LATscan-derived anatomical data	764:794	LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species	764:852	We report LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species and compare these results to those obtained through traditional anatomical techniques.					
36811411	7	18	theme	quantitative	1372:1383	arg1	analyses					1385:1392	both qualitative and quantitative analyses	1351:1392	both qualitative and quantitative analyses	1351:1392	LATscan generate high-quality 2D images and 3D reconstructions of woody plant samples, therefore this new technology is useful for both qualitative and quantitative analyses.					
36811411	7	19	theme	samples	1298:1304	arg1	images					1253:1258	high-quality 2D images	1237:1258	high-quality 2D images	1237:1258	LATscan generate high-quality 2D images and 3D reconstructions of woody plant samples, therefore this new technology is useful for both qualitative and quantitative analyses.					
36811411	7	19	theme	samples	1298:1304	arg1	reconstructions					1267:1281	3D reconstructions	1264:1281	3D reconstructions	1264:1281	LATscan generate high-quality 2D images and 3D reconstructions of woody plant samples, therefore this new technology is useful for both qualitative and quantitative analyses.					
36811411	7	20	theme	woody	1286:1290	arg1	samples					1298:1304	woody plant samples	1286:1304	woody plant samples	1286:1304	LATscan generate high-quality 2D images and 3D reconstructions of woody plant samples, therefore this new technology is useful for both qualitative and quantitative analyses.					
36811411	0	21	theme	woody	76:80	arg1	plants					82:87	woody plants	76:87	woody plants	76:87	Laser ablation tomography (LATscan) as a new tool for anatomical studies of woody plants.					
36811411	8	22	theme	wood	1516:1519	arg1	anatomy					1521:1527	wood anatomy	1516:1527	wood anatomy	1516:1527	This high-throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.					
36811411	6	23	theme	unstained	1201:1209	arg1	samples					1211:1217	unstained samples	1201:1217	unstained samples	1201:1217	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	7	24	theme	3D	1264:1265	arg1	reconstructions					1267:1281	3D reconstructions	1264:1281	3D reconstructions	1264:1281	LATscan generate high-quality 2D images and 3D reconstructions of woody plant samples, therefore this new technology is useful for both qualitative and quantitative analyses.					
36811411	4	25	theme	tissues	728:734	arg1	structure					709:717	the structure	705:717	the structure of woody tissues	705:734	This method has proven useful for studying the structure of delicate plant tissues, however its utility in understanding the structure of woody tissues is underexplored.					
36811411	1	26	theme	sectioning	148:157	arg1	samples					159:165	carefully sectioning samples	138:165	carefully sectioning samples	138:165	Traditionally, botanists study plant anatomy by carefully sectioning samples, histological staining to highlight tissues of interests, then imaging slides under light microscopy.					
36811411	0	27	theme	ablation	6:13	arg1	tomography					15:24	Laser ablation tomography	0:24	Laser ablation tomography (LATscan)	0:34	Laser ablation tomography (LATscan) as a new tool for anatomical studies of woody plants.					
36811411	0	27	theme	ablation	6:13	arg1	LATscan					27:33	LATscan	27:33	LATscan	27:33	Laser ablation tomography (LATscan) as a new tool for anatomical studies of woody plants.					
36811411	6	28	theme	differential	1165:1176	arg1	signals					1190:1196	differential fluorescent signals	1165:1196	differential fluorescent signals	1165:1196	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	0	29	theme	Laser	0:4	arg1	tomography					15:24	Laser ablation tomography	0:24	Laser ablation tomography (LATscan)	0:34	Laser ablation tomography (LATscan) as a new tool for anatomical studies of woody plants.					
36811411	0	29	theme	Laser	0:4	arg1	LATscan					27:33	LATscan	27:33	LATscan	27:33	Laser ablation tomography (LATscan) as a new tool for anatomical studies of woody plants.					
36811411	1	30	theme	light	251:255	arg1	microscopy					257:266	light microscopy	251:266	light microscopy	251:266	Traditionally, botanists study plant anatomy by carefully sectioning samples, histological staining to highlight tissues of interests, then imaging slides under light microscopy.					
36811411	2	31	theme	significant	293:303	arg1	details					305:311	significant details	293:311	significant details	293:311	This approach generates significant details; however, this workflow is laborious, particularly in woody vines (lianas) with heterogeneous anatomies, and ultimately yields two-dimensional (2D) images.					
36811411	4	32	theme	plant	653:657	arg1	tissues					659:665	delicate plant tissues	644:665	delicate plant tissues	644:665	This method has proven useful for studying the structure of delicate plant tissues, however its utility in understanding the structure of woody tissues is underexplored.					
36811411	5	33	theme	anatomical	780:789	arg1	data					791:794	LATscan-derived anatomical data	764:794	LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species	764:852	We report LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species and compare these results to those obtained through traditional anatomical techniques.					
36811411	3	34	theme	high-throughput	510:524	arg1	Tomography					484:493	Laser Ablation Tomography	469:493	Laser Ablation Tomography (LATscan)	469:503	Laser Ablation Tomography (LATscan) is a high-throughput imaging system that yields hundreds of images per minute.					
36811411	3	34	theme	high-throughput	510:524	arg1	system					534:539	a high-throughput imaging system	508:539	a high-throughput imaging system that yields hundreds of images per minute	508:581	Laser Ablation Tomography (LATscan) is a high-throughput imaging system that yields hundreds of images per minute.					
36811411	6	35	theme	fluorescent	1178:1188	arg1	signals					1190:1196	differential fluorescent signals	1165:1196	differential fluorescent signals	1165:1196	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	1	36	theme	histological	168:179	arg1	staining					181:188	histological staining	168:188	histological staining	168:188	Traditionally, botanists study plant anatomy by carefully sectioning samples, histological staining to highlight tissues of interests, then imaging slides under light microscopy.					
36811411	2	37	theme	heterogeneous	393:405	arg1	anatomies					407:415	heterogeneous anatomies	393:415	heterogeneous anatomies	393:415	This approach generates significant details; however, this workflow is laborious, particularly in woody vines (lianas) with heterogeneous anatomies, and ultimately yields two-dimensional (2D) images.					
36811411	8	38	theme	other	1534:1538	arg1	systems					1551:1557	other biological systems	1534:1557	other biological systems	1534:1557	This high-throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.					
36811411	8	39	theme	imaging	1416:1422	arg1	technology					1424:1433	This high-throughput imaging technology	1395:1433	This high-throughput imaging technology	1395:1433	This high-throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.					
36811411	3	40	theme	imaging	526:532	arg1	Tomography					484:493	Laser Ablation Tomography	469:493	Laser Ablation Tomography (LATscan)	469:503	Laser Ablation Tomography (LATscan) is a high-throughput imaging system that yields hundreds of images per minute.					
36811411	3	40	theme	imaging	526:532	arg1	system					534:539	a high-throughput imaging system	508:539	a high-throughput imaging system that yields hundreds of images per minute	508:581	Laser Ablation Tomography (LATscan) is a high-throughput imaging system that yields hundreds of images per minute.					
36811411	6	41	theme	composition	1109:1119	arg1	recognition					1075:1085	the recognition	1071:1085	the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples	1071:1217	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	5	42	theme	anatomical	918:927	arg1	techniques					929:938	traditional anatomical techniques	906:938	traditional anatomical techniques	906:938	We report LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species and compare these results to those obtained through traditional anatomical techniques.					
36811411	7	43	theme	2D	1250:1251	arg1	images					1253:1258	high-quality 2D images	1237:1258	high-quality 2D images	1237:1258	LATscan generate high-quality 2D images and 3D reconstructions of woody plant samples, therefore this new technology is useful for both qualitative and quantitative analyses.					
36811411	5	44	dep	20 mm	830:834	arg1	ca.					826:828	ca.	826:828	ca.	826:828	We report LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species and compare these results to those obtained through traditional anatomical techniques.					
36811411	6	45	dep	composition	1109:1119	arg1	cellulose					1145:1153	cellulose	1145:1153	cellulose	1145:1153	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	6	45	dep	composition	1109:1119	arg1	suberin					1136:1142	suberin	1136:1142	suberin	1136:1142	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	6	45	dep	composition	1109:1119	arg1	lignin					1128:1133	lignin	1128:1133	lignin	1128:1133	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	6	46	theme	cell	1026:1029	arg1	type					1031:1034	cell type	1026:1034	cell type	1026:1034	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	2	47	theme	two-dimensional	440:454	arg1	images					461:466	two-dimensional (2D) images	440:466	two-dimensional (2D) images	440:466	This approach generates significant details; however, this workflow is laborious, particularly in woody vines (lianas) with heterogeneous anatomies, and ultimately yields two-dimensional (2D) images.					
36811411	8	48	theme	systems	1551:1557	arg1	phenotyping					1464:1474	phenotyping	1464:1474	phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems	1464:1557	This high-throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.					
36811411	3	49	theme	images	565:570	arg1	hundreds					553:560	hundreds	553:560	hundreds of images per minute	553:581	Laser Ablation Tomography (LATscan) is a high-throughput imaging system that yields hundreds of images per minute.					
36811411	5	50	theme	lianas	818:823	arg1	stems					809:813	several stems	801:813	several stems of lianas (ca. 20 mm) of seven species	801:852	We report LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species and compare these results to those obtained through traditional anatomical techniques.					
36811411	8	51	theme	biological	1540:1549	arg1	systems					1551:1557	other biological systems	1534:1557	other biological systems	1534:1557	This high-throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.					
36811411	8	52	theme	anatomy	1521:1527	arg1	phenotyping					1464:1474	phenotyping	1464:1474	phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems	1464:1557	This high-throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.					
36811411	7	53	theme	new	1322:1324	arg1	technology					1326:1335	this new technology	1317:1335	this new technology	1317:1335	LATscan generate high-quality 2D images and 3D reconstructions of woody plant samples, therefore this new technology is useful for both qualitative and quantitative analyses.					
36811411	6	54	theme	distinct	1090:1097	arg1	composition					1109:1119	distinct cell wall composition	1090:1119	distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples	1090:1217	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	7	55	theme	plant	1292:1296	arg1	samples					1298:1304	woody plant samples	1286:1304	woody plant samples	1286:1304	LATscan generate high-quality 2D images and 3D reconstructions of woody plant samples, therefore this new technology is useful for both qualitative and quantitative analyses.					
36811411	7	56	theme	high-quality	1237:1248	arg1	images					1253:1258	high-quality 2D images	1237:1258	high-quality 2D images	1237:1258	LATscan generate high-quality 2D images and 3D reconstructions of woody plant samples, therefore this new technology is useful for both qualitative and quantitative analyses.					
36811411	5	57	from	stems	809:813	arg1	data					791:794	LATscan-derived anatomical data	764:794	LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species	764:852	We report LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species and compare these results to those obtained through traditional anatomical techniques.					
36811411	6	58	theme	wall	1104:1107	arg1	composition					1109:1119	distinct cell wall composition	1090:1119	distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples	1090:1217	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	0	59	dep	tomography	15:24	arg1	tool					45:48	a new tool	39:48	a new tool for anatomical studies of woody plants	39:87	Laser ablation tomography (LATscan) as a new tool for anatomical studies of woody plants.					
36811411	6	60	theme	composition	995:1005	arg1	description					973:983	the description	969:983	the description of tissue composition	969:1005	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	6	61	dep	lignin	1128:1133	arg1	e.g.					1122:1125	e.g.	1122:1125	e.g.	1122:1125	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	5	62	link	LATscan-derived	764:778	arg1	data					791:794	LATscan-derived anatomical data	764:794	LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species	764:852	We report LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species and compare these results to those obtained through traditional anatomical techniques.					
36811411	5	63	theme	several	801:807	arg1	stems					809:813	several stems	801:813	several stems of lianas (ca. 20 mm) of seven species	801:852	We report LATscan-derived anatomical data from several stems of lianas (ca. 20 mm) of seven species and compare these results to those obtained through traditional anatomical techniques.					
36811411	4	64	theme	delicate	644:651	arg1	tissues					659:665	delicate plant tissues	644:665	delicate plant tissues	644:665	This method has proven useful for studying the structure of delicate plant tissues, however its utility in understanding the structure of woody tissues is underexplored.					
36811411	2	65	theme	woody	367:371	arg1	lianas					380:385	lianas	380:385	lianas	380:385	This approach generates significant details; however, this workflow is laborious, particularly in woody vines (lianas) with heterogeneous anatomies, and ultimately yields two-dimensional (2D) images.					
36811411	2	65	theme	woody	367:371	arg1	vines					373:377	woody vines	367:377	woody vines (lianas) with heterogeneous anatomies	367:415	This approach generates significant details; however, this workflow is laborious, particularly in woody vines (lianas) with heterogeneous anatomies, and ultimately yields two-dimensional (2D) images.					
36811411	1	66	theme	interests	214:222	arg1	tissues					203:209	tissues	203:209	tissues of interests	203:222	Traditionally, botanists study plant anatomy by carefully sectioning samples, histological staining to highlight tissues of interests, then imaging slides under light microscopy.					
36811411	0	67	theme	new	41:43	arg1	tool					45:48	a new tool	39:48	a new tool for anatomical studies of woody plants	39:87	Laser ablation tomography (LATscan) as a new tool for anatomical studies of woody plants.					
36811411	6	68	theme	cell	1099:1102	arg1	composition					1109:1119	distinct cell wall composition	1090:1119	distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples	1090:1217	LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g., lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.					
36811411	1	69	theme	plant	121:125	arg1	anatomy					127:133	plant anatomy	121:133	plant anatomy	121:133	Traditionally, botanists study plant anatomy by carefully sectioning samples, histological staining to highlight tissues of interests, then imaging slides under light microscopy.					
36811411	4	70	dep	tissues	659:665	arg1	underexplored					739:751	underexplored	739:751	is underexplored	736:751	This method has proven useful for studying the structure of delicate plant tissues, however its utility in understanding the structure of woody tissues is underexplored.					
35881531	0	0	with	Profiles	30:37	arg1	States					113:118	Different Colloidal States	93:118	Different Colloidal States	93:118	In Vitro Colonic Fermentation Profiles and Microbial Responses of Cellulose Derivatives with Different Colloidal States.					
35881531	4	1	theme	particle	689:696	arg1	size					698:701	reduced particle size	681:701	reduced particle size	681:701	The results suggested that reduced particle size and increased aspect ratio improved the fermentability of insoluble cellulose derivatives.					
35881531	8	2	theme	microbial	1239:1247	arg1	composition					1259:1269	microbial community composition	1239:1269	microbial community composition	1239:1269	The findings demonstrated that the colloidal states of cellulose derivatives, such as size and solubility, were important factors governing microbial community composition and metabolites.					
35881531	0	3	theme	Derivatives	76:86	arg1	Profiles					30:37	In Vitro Colonic Fermentation Profiles	0:37	In Vitro Colonic Fermentation Profiles	0:37	In Vitro Colonic Fermentation Profiles and Microbial Responses of Cellulose Derivatives with Different Colloidal States.					
35881531	0	3	theme	Derivatives	76:86	arg1	Responses					53:61	Microbial Responses	43:61	Microbial Responses	43:61	In Vitro Colonic Fermentation Profiles and Microbial Responses of Cellulose Derivatives with Different Colloidal States.					
35881531	4	4	theme	reduced	681:687	arg1	size					698:701	reduced particle size	681:701	reduced particle size	681:701	The results suggested that reduced particle size and increased aspect ratio improved the fermentability of insoluble cellulose derivatives.					
35881531	0	5	theme	Colloidal	103:111	arg1	States					113:118	Different Colloidal States	93:118	Different Colloidal States	93:118	In Vitro Colonic Fermentation Profiles and Microbial Responses of Cellulose Derivatives with Different Colloidal States.					
35881531	4	6	theme	insoluble	761:769	arg1	derivatives					781:791	insoluble cellulose derivatives	761:791	insoluble cellulose derivatives	761:791	The results suggested that reduced particle size and increased aspect ratio improved the fermentability of insoluble cellulose derivatives.					
35881531	0	7	theme	Different	93:101	arg1	States					113:118	Different Colloidal States	93:118	Different Colloidal States	93:118	In Vitro Colonic Fermentation Profiles and Microbial Responses of Cellulose Derivatives with Different Colloidal States.					
35881531	4	8	theme	increased	707:715	arg1	ratio					724:728	increased aspect ratio	707:728	increased aspect ratio	707:728	The results suggested that reduced particle size and increased aspect ratio improved the fermentability of insoluble cellulose derivatives.					
35881531	5	9	theme	total	841:845	arg1	production					853:862	similar total SCFAs production	833:862	similar total SCFAs production	833:862	MCC and CMC were barely fermented with similar total SCFAs production as the blank.					
35881531	4	10	theme	derivatives	781:791	arg1	fermentability					743:756	the fermentability	739:756	the fermentability of insoluble cellulose derivatives	739:791	The results suggested that reduced particle size and increased aspect ratio improved the fermentability of insoluble cellulose derivatives.					
35881531	1	11	theme	food	178:181	arg1	industry					183:190	the food industry	174:190	the food industry	174:190	Although cellulose derivatives are widely applied in the food industry, the effects of their structural properties on colonic health is unknown.					
35881531	6	12	theme	rRNA	882:885	arg1	sequencing					887:896	16S rRNA sequencing	878:896	16S rRNA sequencing	878:896	16S rRNA sequencing revealed that the fermentation of cellulose derivatives resulted in divergent microbial community structures.					
35881531	4	13	theme	cellulose	771:779	arg1	derivatives					781:791	insoluble cellulose derivatives	761:791	insoluble cellulose derivatives	761:791	The results suggested that reduced particle size and increased aspect ratio improved the fermentability of insoluble cellulose derivatives.					
35881531	8	14	theme	colloidal	1134:1142	arg1	factors					1221:1227	important factors	1211:1227	important factors governing microbial community composition and metabolites	1211:1285	The findings demonstrated that the colloidal states of cellulose derivatives, such as size and solubility, were important factors governing microbial community composition and metabolites.					
35881531	8	14	theme	colloidal	1134:1142	arg1	states					1144:1149	the colloidal states	1130:1149	the colloidal states of cellulose derivatives, such as size and solubility,	1130:1204	The findings demonstrated that the colloidal states of cellulose derivatives, such as size and solubility, were important factors governing microbial community composition and metabolites.					
35881531	6	15	theme	16S	878:880	arg1	sequencing					887:896	16S rRNA sequencing	878:896	16S rRNA sequencing	878:896	16S rRNA sequencing revealed that the fermentation of cellulose derivatives resulted in divergent microbial community structures.					
35881531	0	16	theme	In	0:1	arg1	Profiles					30:37	In Vitro Colonic Fermentation Profiles	0:37	In Vitro Colonic Fermentation Profiles	0:37	In Vitro Colonic Fermentation Profiles and Microbial Responses of Cellulose Derivatives with Different Colloidal States.					
35881531	6	17	theme	cellulose	932:940	arg1	derivatives					942:952	cellulose derivatives	932:952	cellulose derivatives	932:952	16S rRNA sequencing revealed that the fermentation of cellulose derivatives resulted in divergent microbial community structures.					
35881531	3	18	theme	highest	574:580	arg1	production					582:591	the highest production	570:591	the highest production of total short-chain fatty acids (SCFAs)	570:632	TOCNF exhibited the highest production of total short-chain fatty acids (SCFAs), followed by TOCNC.					
35881531	0	19	theme	Fermentation	17:28	arg1	Profiles					30:37	In Vitro Colonic Fermentation Profiles	0:37	In Vitro Colonic Fermentation Profiles	0:37	In Vitro Colonic Fermentation Profiles and Microbial Responses of Cellulose Derivatives with Different Colloidal States.					
35881531	6	20	theme	community	986:994	arg1	structures					996:1005	divergent microbial community structures	966:1005	divergent microbial community structures	966:1005	16S rRNA sequencing revealed that the fermentation of cellulose derivatives resulted in divergent microbial community structures.					
35881531	2	21	dep	in	522:523	arg1	vitro					525:529	vitro	525:529	vitro	525:529	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	22	theme	derivatives	296:306	arg1	types					277:281	four types	272:281	four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC)	272:485	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	5	23	theme	similar	833:839	arg1	production					853:862	similar total SCFAs production	833:862	similar total SCFAs production	833:862	MCC and CMC were barely fermented with similar total SCFAs production as the blank.					
35881531	0	24	theme	Colonic	9:15	arg1	Profiles					30:37	In Vitro Colonic Fermentation Profiles	0:37	In Vitro Colonic Fermentation Profiles	0:37	In Vitro Colonic Fermentation Profiles and Microbial Responses of Cellulose Derivatives with Different Colloidal States.					
35881531	6	25	theme	microbial	976:984	arg1	structures					996:1005	divergent microbial community structures	966:1005	divergent microbial community structures	966:1005	16S rRNA sequencing revealed that the fermentation of cellulose derivatives resulted in divergent microbial community structures.					
35881531	2	26	theme	cellulose	286:294	arg1	derivatives					296:306	cellulose derivatives	286:306	cellulose derivatives	286:306	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	26	theme	cellulose	286:294	arg1	cellulose					384:392	TEMPO-oxidized nanofibrillated cellulose	353:392	TEMPO-oxidized nanofibrillated cellulose (TOCNF)	353:400	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	26	theme	cellulose	286:294	arg1	cellulose					471:479	carboxymethyl cellulose	457:479	carboxymethyl cellulose (CMC)	457:485	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	26	theme	cellulose	286:294	arg1	cellulose					336:344	microcrystalline cellulose	319:344	microcrystalline cellulose (MCC)	319:350	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	26	theme	cellulose	286:294	arg1	cellulose					434:442	TEMPO-oxidized nanocrystalline cellulose	403:442	TEMPO-oxidized nanocrystalline cellulose (TOCNC)	403:450	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	27	theme	nanocrystalline	418:432	arg1	TOCNC					445:449	TOCNC	445:449	TOCNC	445:449	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	27	theme	nanocrystalline	418:432	arg1	cellulose					434:442	TEMPO-oxidized nanocrystalline cellulose	403:442	TEMPO-oxidized nanocrystalline cellulose (TOCNC)	403:450	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	6	28	theme	divergent	966:974	arg1	structures					996:1005	divergent microbial community structures	966:1005	divergent microbial community structures	966:1005	16S rRNA sequencing revealed that the fermentation of cellulose derivatives resulted in divergent microbial community structures.					
35881531	2	29	theme	fermentation	531:542	arg1	profiles					544:551	their in vitro fermentation profiles	516:551	their in vitro fermentation profiles	516:551	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	5	30	theme	SCFAs	847:851	arg1	production					853:862	similar total SCFAs production	833:862	similar total SCFAs production	833:862	MCC and CMC were barely fermented with similar total SCFAs production as the blank.					
35881531	3	31	theme	total	596:600	arg1	acids					620:624	total short-chain fatty acids	596:624	total short-chain fatty acids (SCFAs)	596:632	TOCNF exhibited the highest production of total short-chain fatty acids (SCFAs), followed by TOCNC.					
35881531	3	31	theme	total	596:600	arg1	SCFAs					627:631	SCFAs	627:631	SCFAs	627:631	TOCNF exhibited the highest production of total short-chain fatty acids (SCFAs), followed by TOCNC.					
35881531	2	32	theme	TEMPO-oxidized	403:416	arg1	TOCNC					445:449	TOCNC	445:449	TOCNC	445:449	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	32	theme	TEMPO-oxidized	403:416	arg1	cellulose					434:442	TEMPO-oxidized nanocrystalline cellulose	403:442	TEMPO-oxidized nanocrystalline cellulose (TOCNC)	403:450	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	8	33	theme	community	1249:1257	arg1	composition					1259:1269	microbial community composition	1239:1269	microbial community composition	1239:1269	The findings demonstrated that the colloidal states of cellulose derivatives, such as size and solubility, were important factors governing microbial community composition and metabolites.					
35881531	3	34	theme	short-chain	602:612	arg1	acids					620:624	total short-chain fatty acids	596:624	total short-chain fatty acids (SCFAs)	596:632	TOCNF exhibited the highest production of total short-chain fatty acids (SCFAs), followed by TOCNC.					
35881531	3	34	theme	short-chain	602:612	arg1	SCFAs					627:631	SCFAs	627:631	SCFAs	627:631	TOCNF exhibited the highest production of total short-chain fatty acids (SCFAs), followed by TOCNC.					
35881531	7	35	theme	high	1054:1057	arg1	specificity					1059:1069	high specificity to utilize TOCNF and TOCNC	1054:1096	high specificity to utilize TOCNF and TOCNC	1054:1096	Moreover, Bacteroides cellulosilyticus showed high specificity to utilize TOCNF and TOCNC.					
35881531	1	36	theme	structural	214:223	arg1	properties					225:234	their structural properties	208:234	their structural properties	208:234	Although cellulose derivatives are widely applied in the food industry, the effects of their structural properties on colonic health is unknown.					
35881531	2	37	theme	carboxymethyl	457:469	arg1	CMC					482:484	CMC	482:484	CMC	482:484	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	37	theme	carboxymethyl	457:469	arg1	cellulose					471:479	carboxymethyl cellulose	457:479	carboxymethyl cellulose (CMC)	457:485	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	38	theme	in	522:523	arg1	profiles					544:551	their in vitro fermentation profiles	516:551	their in vitro fermentation profiles	516:551	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	0	39	theme	Microbial	43:51	arg1	Responses					53:61	Microbial Responses	43:61	Microbial Responses	43:61	In Vitro Colonic Fermentation Profiles and Microbial Responses of Cellulose Derivatives with Different Colloidal States.					
35881531	6	40	theme	derivatives	942:952	arg1	fermentation					916:927	the fermentation	912:927	the fermentation of cellulose derivatives	912:952	16S rRNA sequencing revealed that the fermentation of cellulose derivatives resulted in divergent microbial community structures.					
35881531	1	41	from	effects	197:203	arg1	health					247:252	colonic health	239:252	colonic health	239:252	Although cellulose derivatives are widely applied in the food industry, the effects of their structural properties on colonic health is unknown.					
35881531	1	42	theme	cellulose	130:138	arg1	derivatives					140:150	cellulose derivatives	130:150	cellulose derivatives	130:150	Although cellulose derivatives are widely applied in the food industry, the effects of their structural properties on colonic health is unknown.					
35881531	3	43	theme	acids	620:624	arg1	production					582:591	the highest production	570:591	the highest production of total short-chain fatty acids (SCFAs)	570:632	TOCNF exhibited the highest production of total short-chain fatty acids (SCFAs), followed by TOCNC.					
35881531	8	44	theme	derivatives	1164:1174	arg1	factors					1221:1227	important factors	1211:1227	important factors governing microbial community composition and metabolites	1211:1285	The findings demonstrated that the colloidal states of cellulose derivatives, such as size and solubility, were important factors governing microbial community composition and metabolites.					
35881531	8	44	theme	derivatives	1164:1174	arg1	states					1144:1149	the colloidal states	1130:1149	the colloidal states of cellulose derivatives, such as size and solubility,	1130:1204	The findings demonstrated that the colloidal states of cellulose derivatives, such as size and solubility, were important factors governing microbial community composition and metabolites.					
35881531	4	45	theme	aspect	717:722	arg1	ratio					724:728	increased aspect ratio	707:728	increased aspect ratio	707:728	The results suggested that reduced particle size and increased aspect ratio improved the fermentability of insoluble cellulose derivatives.					
35881531	1	46	theme	properties	225:234	arg1	unknown					257:263	unknown	257:263	unknown	257:263	Although cellulose derivatives are widely applied in the food industry, the effects of their structural properties on colonic health is unknown.					
35881531	1	46	theme	properties	225:234	arg1	effects					197:203	the effects	193:203	the effects of their structural properties on colonic health	193:252	Although cellulose derivatives are widely applied in the food industry, the effects of their structural properties on colonic health is unknown.					
35881531	8	47	theme	cellulose	1154:1162	arg1	solubility					1194:1203	solubility	1194:1203	solubility	1194:1203	The findings demonstrated that the colloidal states of cellulose derivatives, such as size and solubility, were important factors governing microbial community composition and metabolites.					
35881531	8	47	theme	cellulose	1154:1162	arg1	size					1185:1188	size	1185:1188	size	1185:1188	The findings demonstrated that the colloidal states of cellulose derivatives, such as size and solubility, were important factors governing microbial community composition and metabolites.					
35881531	8	47	theme	cellulose	1154:1162	arg1	derivatives					1164:1174	cellulose derivatives	1154:1174	cellulose derivatives	1154:1174	The findings demonstrated that the colloidal states of cellulose derivatives, such as size and solubility, were important factors governing microbial community composition and metabolites.					
35881531	8	48	theme	important	1211:1219	arg1	factors					1221:1227	important factors	1211:1227	important factors governing microbial community composition and metabolites	1211:1285	The findings demonstrated that the colloidal states of cellulose derivatives, such as size and solubility, were important factors governing microbial community composition and metabolites.					
35881531	8	48	theme	important	1211:1219	arg1	states					1144:1149	the colloidal states	1130:1149	the colloidal states of cellulose derivatives, such as size and solubility,	1130:1204	The findings demonstrated that the colloidal states of cellulose derivatives, such as size and solubility, were important factors governing microbial community composition and metabolites.					
35881531	0	49	with	Responses	53:61	arg1	States					113:118	Different Colloidal States	93:118	Different Colloidal States	93:118	In Vitro Colonic Fermentation Profiles and Microbial Responses of Cellulose Derivatives with Different Colloidal States.					
35881531	2	50	theme	microcrystalline	319:334	arg1	MCC					347:349	MCC	347:349	MCC	347:349	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	50	theme	microcrystalline	319:334	arg1	cellulose					336:344	microcrystalline cellulose	319:344	microcrystalline cellulose (MCC)	319:350	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	0	51	theme	Cellulose	66:74	arg1	Derivatives					76:86	Cellulose Derivatives	66:86	Cellulose Derivatives	66:86	In Vitro Colonic Fermentation Profiles and Microbial Responses of Cellulose Derivatives with Different Colloidal States.					
35881531	3	52	theme	fatty	614:618	arg1	acids					620:624	total short-chain fatty acids	596:624	total short-chain fatty acids (SCFAs)	596:632	TOCNF exhibited the highest production of total short-chain fatty acids (SCFAs), followed by TOCNC.					
35881531	3	52	theme	fatty	614:618	arg1	SCFAs					627:631	SCFAs	627:631	SCFAs	627:631	TOCNF exhibited the highest production of total short-chain fatty acids (SCFAs), followed by TOCNC.					
35881531	7	53	theme	Bacteroides	1018:1028	arg1	cellulosilyticus					1030:1045	Bacteroides cellulosilyticus	1018:1045	Bacteroides cellulosilyticus	1018:1045	Moreover, Bacteroides cellulosilyticus showed high specificity to utilize TOCNF and TOCNC.					
35881531	2	54	theme	nanofibrillated	368:382	arg1	TOCNF					395:399	TOCNF	395:399	TOCNF	395:399	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	54	theme	nanofibrillated	368:382	arg1	cellulose					384:392	TEMPO-oxidized nanofibrillated cellulose	353:392	TEMPO-oxidized nanofibrillated cellulose (TOCNF)	353:400	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	1	55	theme	colonic	239:245	arg1	health					247:252	colonic health	239:252	colonic health	239:252	Although cellulose derivatives are widely applied in the food industry, the effects of their structural properties on colonic health is unknown.					
35881531	0	56	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Colonic Fermentation Profiles and Microbial Responses of Cellulose Derivatives with Different Colloidal States.					
35881531	2	57	theme	TEMPO-oxidized	353:366	arg1	TOCNF					395:399	TOCNF	395:399	TOCNF	395:399	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35881531	2	57	theme	TEMPO-oxidized	353:366	arg1	cellulose					384:392	TEMPO-oxidized nanofibrillated cellulose	353:392	TEMPO-oxidized nanofibrillated cellulose (TOCNF)	353:400	Here, four types of cellulose derivatives, including microcrystalline cellulose (MCC), TEMPO-oxidized nanofibrillated cellulose (TOCNF), TEMPO-oxidized nanocrystalline cellulose (TOCNC), and carboxymethyl cellulose (CMC) were selected to investigate their in vitro fermentation profiles.					
35127946	0	0	theme	pseudocatenulatum	93:109	arg1	Effect					67:72	the Better Protective Effect	45:72	the Better Protective Effect of Bifidobacterium pseudocatenulatum LI09 against Rat Liver Injury	45:139	Multiple Intestinal Bacteria Associated with the Better Protective Effect of Bifidobacterium pseudocatenulatum LI09 against Rat Liver Injury.					
35127946	5	1	theme	ALB	659:661	arg1	level					650:654	Higher level	643:654	Higher level of ALB	643:661	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	5	1	theme	ALB	659:661	arg1	levels					673:678	lower levels	667:678	lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α	667:717	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	7	2	theme	intestinal	978:987	arg1	different					1016:1024	different	1016:1024	different	1016:1024	The intestinal microbiota composition was different between the two cohorts.					
35127946	7	2	theme	intestinal	978:987	arg1	composition					1000:1010	The intestinal microbiota composition	974:1010	The intestinal microbiota composition	974:1010	The intestinal microbiota composition was different between the two cohorts.					
35127946	10	3	theme	microbiota	1536:1545	arg1	network					1547:1553	the microbiota network	1532:1553	the microbiota network of the nonsevere cohort	1532:1577	Among them, ASV135_Lachnospiraceae_NK4A136 was the most powerful gatekeeper in the microbiota network of the nonsevere cohort.					
35127946	6	4	theme	nonsevere	944:952	arg1	cohorts					965:971	the nonsevere and severe cohorts	940:971	the nonsevere and severe cohorts	940:971	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	0	5	theme	Bifidobacterium	77:91	arg1	pseudocatenulatum					93:109	Bifidobacterium pseudocatenulatum LI09	77:114	Bifidobacterium pseudocatenulatum LI09	77:114	Multiple Intestinal Bacteria Associated with the Better Protective Effect of Bifidobacterium pseudocatenulatum LI09 against Rat Liver Injury.					
35127946	12	6	theme	LI09	1834:1837	arg1	effectiveness					1817:1829	the effectiveness	1813:1829	the effectiveness of LI09	1813:1837	They were likely to enhance the effectiveness of LI09, and their clinical application deserves further investigation.					
35127946	12	7	theme	further	1880:1886	arg1	investigation					1888:1900	further investigation	1880:1900	further investigation	1880:1900	They were likely to enhance the effectiveness of LI09, and their clinical application deserves further investigation.					
35127946	9	8	theme	microbiota	1413:1422	arg1	networks					1424:1431	the intestinal microbiota networks	1398:1431	the intestinal microbiota networks of the two cohorts	1398:1450	At the structure level, two groups of phylotypes with most correlations were determined in the intestinal microbiota networks of the two cohorts.					
35127946	5	9	theme	lower	667:671	arg1	levels					673:678	lower levels	667:678	lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α	667:717	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	2	10	from	injury	332:337	arg1	difference					274:283	individual difference	263:283	individual difference in the protective effects of LI09 on the liver injury	263:337	However, individual difference in the protective effects of LI09 on the liver injury remains poorly understood.					
35127946	11	11	theme	better	1711:1716	arg1	effect					1729:1734	the better protective effect	1707:1734	the better protective effect of LI09 against D-GalN-induced rat liver injury	1707:1782	In conclusion, some intestinal bacteria, e.g., Lachnospiraceae_NK4A136, Parabacteroides, and Clostridium, were associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	5	12	theme	IL-5	702:705	arg1	level					650:654	Higher level	643:654	Higher level of ALB	643:661	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	5	12	theme	IL-5	702:705	arg1	levels					673:678	lower levels	667:678	lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α	667:717	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	9	13	with	phylotypes	1345:1354	arg1	correlations					1366:1377	most correlations	1361:1377	most correlations	1361:1377	At the structure level, two groups of phylotypes with most correlations were determined in the intestinal microbiota networks of the two cohorts.					
35127946	2	14	theme	LI09	314:317	arg1	effects					303:309	the protective effects	288:309	the protective effects of LI09 on the liver injury	288:337	However, individual difference in the protective effects of LI09 on the liver injury remains poorly understood.					
35127946	0	15	theme	Liver	128:132	arg1	Injury					134:139	Rat Liver Injury	124:139	Rat Liver Injury	124:139	Multiple Intestinal Bacteria Associated with the Better Protective Effect of Bifidobacterium pseudocatenulatum LI09 against Rat Liver Injury.					
35127946	4	16	dep	cohorts	583:589	arg1	i.e.					552:555	i.e.	552:555	i.e.	552:555	Two rat cohorts, i.e., the nonsevere and severe cohorts, were divided based on their liver injury severity.					
35127946	3	17	theme	D-GalN-induced	502:515	arg1	injury					527:532	D-GalN-induced rat liver injury	502:532	D-GalN-induced rat liver injury	502:532	The present study is aimed at determining the multiple intestinal bacteria associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	3	18	theme	protective	468:477	arg1	effect					479:484	the better protective effect	457:484	the better protective effect of LI09 against D-GalN-induced rat liver injury	457:532	The present study is aimed at determining the multiple intestinal bacteria associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	0	19	theme	Rat	124:126	arg1	Injury					134:139	Rat Liver Injury	124:139	Rat Liver Injury	124:139	Multiple Intestinal Bacteria Associated with the Better Protective Effect of Bifidobacterium pseudocatenulatum LI09 against Rat Liver Injury.					
35127946	9	20	theme	structure	1314:1322	arg1	level					1324:1328	the structure level	1310:1328	the structure level	1310:1328	At the structure level, two groups of phylotypes with most correlations were determined in the intestinal microbiota networks of the two cohorts.					
35127946	5	21	theme	Higher	643:648	arg1	level					650:654	Higher level	643:654	Higher level of ALB	643:661	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	0	22	theme	Multiple	0:7	arg1	Bacteria					20:27	Multiple Intestinal Bacteria	0:27	Multiple Intestinal Bacteria	0:27	Multiple Intestinal Bacteria Associated with the Better Protective Effect of Bifidobacterium pseudocatenulatum LI09 against Rat Liver Injury.					
35127946	10	23	theme	nonsevere	1562:1570	arg1	cohort					1572:1577	the nonsevere cohort	1558:1577	the nonsevere cohort	1558:1577	Among them, ASV135_Lachnospiraceae_NK4A136 was the most powerful gatekeeper in the microbiota network of the nonsevere cohort.					
35127946	3	24	theme	present	370:376	arg1	study					378:382	The present study	366:382	The present study	366:382	The present study is aimed at determining the multiple intestinal bacteria associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	0	25	theme	Intestinal	9:18	arg1	Bacteria					20:27	Multiple Intestinal Bacteria	0:27	Multiple Intestinal Bacteria	0:27	Multiple Intestinal Bacteria Associated with the Better Protective Effect of Bifidobacterium pseudocatenulatum LI09 against Rat Liver Injury.					
35127946	6	26	theme	intestinal	915:924	arg1	microbiota					926:935	the intestinal microbiota	911:935	the intestinal microbiota of the nonsevere and severe cohorts	911:971	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	9	27	theme	cohorts	1444:1450	arg1	networks					1424:1431	the intestinal microbiota networks	1398:1431	the intestinal microbiota networks of the two cohorts	1398:1450	At the structure level, two groups of phylotypes with most correlations were determined in the intestinal microbiota networks of the two cohorts.					
35127946	3	28	theme	multiple	412:419	arg1	bacteria					432:439	the multiple intestinal bacteria	408:439	the multiple intestinal bacteria associated with the better protective effect of LI09 against D-GalN-induced rat liver injury	408:532	The present study is aimed at determining the multiple intestinal bacteria associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	4	29	theme	severe	576:581	arg1	cohorts					583:589	the nonsevere and severe cohorts	558:589	the nonsevere and severe cohorts	558:589	Two rat cohorts, i.e., the nonsevere and severe cohorts, were divided based on their liver injury severity.					
35127946	9	30	theme	most	1361:1364	arg1	correlations					1366:1377	most correlations	1361:1377	most correlations	1361:1377	At the structure level, two groups of phylotypes with most correlations were determined in the intestinal microbiota networks of the two cohorts.					
35127946	3	31	theme	better	461:466	arg1	effect					479:484	the better protective effect	457:484	the better protective effect of LI09 against D-GalN-induced rat liver injury	457:532	The present study is aimed at determining the multiple intestinal bacteria associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	5	32	theme	severe	768:773	arg1	cohort					775:780	the severe cohort	764:780	the severe cohort	764:780	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	10	33	theme	powerful	1509:1516	arg1	gatekeeper					1518:1527	the most powerful gatekeeper	1500:1527	the most powerful gatekeeper	1500:1527	Among them, ASV135_Lachnospiraceae_NK4A136 was the most powerful gatekeeper in the microbiota network of the nonsevere cohort.					
35127946	10	33	theme	powerful	1509:1516	arg1	ASV135_Lachnospiraceae_NK4A136					1465:1494	ASV135_Lachnospiraceae_NK4A136	1465:1494	ASV135_Lachnospiraceae_NK4A136	1465:1494	Among them, ASV135_Lachnospiraceae_NK4A136 was the most powerful gatekeeper in the microbiota network of the nonsevere cohort.					
35127946	2	34	theme	liver	326:330	arg1	injury					332:337	the liver injury	322:337	the liver injury	322:337	However, individual difference in the protective effects of LI09 on the liver injury remains poorly understood.					
35127946	2	35	from	difference	274:283	arg1	effects					303:309	the protective effects	288:309	the protective effects of LI09 on the liver injury	288:337	However, individual difference in the protective effects of LI09 on the liver injury remains poorly understood.					
35127946	2	35	from	difference	274:283	arg1	injury					332:337	the liver injury	322:337	the liver injury	322:337	However, individual difference in the protective effects of LI09 on the liver injury remains poorly understood.					
35127946	6	36	dep	indices	803:809	arg1	Shannon					836:842	Shannon	836:842	Shannon	836:842	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	6	36	dep	indices	803:809	arg1	Pielou					849:854	Pielou	849:854	Pielou	849:854	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	6	36	dep	indices	803:809	arg1	species					827:833	observed species	818:833	observed species	818:833	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	5	37	theme	TBA	693:695	arg1	level					650:654	Higher level	643:654	Higher level of ALB	643:661	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	5	37	theme	TBA	693:695	arg1	levels					673:678	lower levels	667:678	lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α	667:717	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	6	38	theme	observed	818:825	arg1	Shannon					836:842	Shannon	836:842	Shannon	836:842	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	6	38	theme	observed	818:825	arg1	Pielou					849:854	Pielou	849:854	Pielou	849:854	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	6	38	theme	observed	818:825	arg1	species					827:833	observed species	818:833	observed species	818:833	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	11	39	dep	Lachnospiraceae_NK4A136	1627:1649	arg1	e.g.					1621:1624	e.g.	1621:1624	e.g.	1621:1624	In conclusion, some intestinal bacteria, e.g., Lachnospiraceae_NK4A136, Parabacteroides, and Clostridium, were associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	1	40	theme	induced	232:238	arg1	injury					246:251	D-galactosamine- (D-GalN-) induced liver injury	205:251	D-galactosamine- (D-GalN-) induced liver injury	205:251	Bifidobacterium pseudocatenulatum LI09 could protect rats from D-galactosamine- (D-GalN-) induced liver injury.					
35127946	11	41	theme	protective	1718:1727	arg1	effect					1729:1734	the better protective effect	1707:1734	the better protective effect of LI09 against D-GalN-induced rat liver injury	1707:1782	In conclusion, some intestinal bacteria, e.g., Lachnospiraceae_NK4A136, Parabacteroides, and Clostridium, were associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	2	42	theme	individual	263:272	arg1	difference					274:283	individual difference	263:283	individual difference in the protective effects of LI09 on the liver injury	263:337	However, individual difference in the protective effects of LI09 on the liver injury remains poorly understood.					
35127946	6	43	dep	species	827:833	arg1	i.e.					812:815	i.e.	812:815	i.e.	812:815	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	1	44	theme	liver	240:244	arg1	injury					246:251	D-galactosamine- (D-GalN-) induced liver injury	205:251	D-galactosamine- (D-GalN-) induced liver injury	205:251	Bifidobacterium pseudocatenulatum LI09 could protect rats from D-galactosamine- (D-GalN-) induced liver injury.					
35127946	5	45	theme	nonsevere	742:750	arg1	cohort					752:757	the nonsevere cohort	738:757	the nonsevere cohort than the severe cohort	738:780	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	6	46	theme	significant	879:889	arg1	differences					891:901	significant differences	879:901	significant differences between the intestinal microbiota of the nonsevere and severe cohorts	879:971	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	5	47	theme	TB	698:699	arg1	level					650:654	Higher level	643:654	Higher level of ALB	643:661	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	5	47	theme	TB	698:699	arg1	levels					673:678	lower levels	667:678	lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α	667:717	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	11	48	theme	D-GalN-induced	1752:1765	arg1	injury					1777:1782	D-GalN-induced rat liver injury	1752:1782	D-GalN-induced rat liver injury	1752:1782	In conclusion, some intestinal bacteria, e.g., Lachnospiraceae_NK4A136, Parabacteroides, and Clostridium, were associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	9	49	theme	intestinal	1402:1411	arg1	networks					1424:1431	the intestinal microbiota networks	1398:1431	the intestinal microbiota networks of the two cohorts	1398:1450	At the structure level, two groups of phylotypes with most correlations were determined in the intestinal microbiota networks of the two cohorts.					
35127946	3	50	theme	LI09	489:492	arg1	effect					479:484	the better protective effect	457:484	the better protective effect of LI09 against D-GalN-induced rat liver injury	457:532	The present study is aimed at determining the multiple intestinal bacteria associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	0	51	theme	Protective	56:65	arg1	Effect					67:72	the Better Protective Effect	45:72	the Better Protective Effect of Bifidobacterium pseudocatenulatum LI09 against Rat Liver Injury	45:139	Multiple Intestinal Bacteria Associated with the Better Protective Effect of Bifidobacterium pseudocatenulatum LI09 against Rat Liver Injury.					
35127946	9	52	theme	phylotypes	1345:1354	arg1	groups					1335:1340	two groups	1331:1340	two groups of phylotypes with most correlations	1331:1377	At the structure level, two groups of phylotypes with most correlations were determined in the intestinal microbiota networks of the two cohorts.					
35127946	9	52	theme	phylotypes	1345:1354	arg1	phylotypes					1345:1354	phylotypes	1345:1354	phylotypes with most correlations	1345:1377	At the structure level, two groups of phylotypes with most correlations were determined in the intestinal microbiota networks of the two cohorts.					
35127946	7	53	theme	microbiota	989:998	arg1	different					1016:1024	different	1016:1024	different	1016:1024	The intestinal microbiota composition was different between the two cohorts.					
35127946	7	53	theme	microbiota	989:998	arg1	composition					1000:1010	The intestinal microbiota composition	974:1010	The intestinal microbiota composition	974:1010	The intestinal microbiota composition was different between the two cohorts.					
35127946	12	54	theme	clinical	1850:1857	arg1	application					1859:1869	their clinical application	1844:1869	their clinical application	1844:1869	They were likely to enhance the effectiveness of LI09, and their clinical application deserves further investigation.					
35127946	0	55	theme	Better	49:54	arg1	Effect					67:72	the Better Protective Effect	45:72	the Better Protective Effect of Bifidobacterium pseudocatenulatum LI09 against Rat Liver Injury	45:139	Multiple Intestinal Bacteria Associated with the Better Protective Effect of Bifidobacterium pseudocatenulatum LI09 against Rat Liver Injury.					
35127946	4	56	theme	nonsevere	562:570	arg1	cohorts					583:589	the nonsevere and severe cohorts	558:589	the nonsevere and severe cohorts	558:589	Two rat cohorts, i.e., the nonsevere and severe cohorts, were divided based on their liver injury severity.					
35127946	5	57	theme	ALT	683:685	arg1	level					650:654	Higher level	643:654	Higher level of ALB	643:661	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	5	57	theme	ALT	683:685	arg1	levels					673:678	lower levels	667:678	lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α	667:717	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	11	58	theme	LI09	1739:1742	arg1	effect					1729:1734	the better protective effect	1707:1734	the better protective effect of LI09 against D-GalN-induced rat liver injury	1707:1782	In conclusion, some intestinal bacteria, e.g., Lachnospiraceae_NK4A136, Parabacteroides, and Clostridium, were associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	10	59	from	gatekeeper	1518:1527	arg1	network					1547:1553	the microbiota network	1532:1553	the microbiota network of the nonsevere cohort	1532:1577	Among them, ASV135_Lachnospiraceae_NK4A136 was the most powerful gatekeeper in the microbiota network of the nonsevere cohort.					
35127946	10	60	theme	cohort	1572:1577	arg1	network					1547:1553	the microbiota network	1532:1553	the microbiota network of the nonsevere cohort	1532:1577	Among them, ASV135_Lachnospiraceae_NK4A136 was the most powerful gatekeeper in the microbiota network of the nonsevere cohort.					
35127946	6	61	theme	diversity	793:801	arg1	indices					803:809	The alpha diversity indices	783:809	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices)	783:863	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	2	62	theme	protective	292:301	arg1	effects					303:309	the protective effects	288:309	the protective effects of LI09 on the liver injury	288:337	However, individual difference in the protective effects of LI09 on the liver injury remains poorly understood.					
35127946	6	63	theme	cohorts	965:971	arg1	microbiota					926:935	the intestinal microbiota	911:935	the intestinal microbiota of the nonsevere and severe cohorts	911:971	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	1	64	theme	Bifidobacterium	142:156	arg1	pseudocatenulatum					158:174	Bifidobacterium pseudocatenulatum LI09	142:179	Bifidobacterium pseudocatenulatum LI09	142:179	Bifidobacterium pseudocatenulatum LI09 could protect rats from D-galactosamine- (D-GalN-) induced liver injury.					
35127946	2	65	from	effects	303:309	arg1	injury					332:337	the liver injury	322:337	the liver injury	322:337	However, individual difference in the protective effects of LI09 on the liver injury remains poorly understood.					
35127946	4	66	theme	liver	620:624	arg1	severity					633:640	their liver injury severity	614:640	their liver injury severity	614:640	Two rat cohorts, i.e., the nonsevere and severe cohorts, were divided based on their liver injury severity.					
35127946	5	67	theme	AST	688:690	arg1	level					650:654	Higher level	643:654	Higher level of ALB	643:661	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	5	67	theme	AST	688:690	arg1	levels					673:678	lower levels	667:678	lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α	667:717	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	6	68	theme	alpha	787:791	arg1	indices					803:809	The alpha diversity indices	783:809	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices)	783:863	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	11	69	theme	intestinal	1600:1609	arg1	bacteria					1611:1618	some intestinal bacteria	1595:1618	some intestinal bacteria	1595:1618	In conclusion, some intestinal bacteria, e.g., Lachnospiraceae_NK4A136, Parabacteroides, and Clostridium, were associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	3	70	theme	liver	521:525	arg1	injury					527:532	D-GalN-induced rat liver injury	502:532	D-GalN-induced rat liver injury	502:532	The present study is aimed at determining the multiple intestinal bacteria associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	8	71	theme	nonsevere	1218:1226	arg1	cohort					1228:1233	the nonsevere cohort	1214:1233	the nonsevere cohort	1214:1233	Ten phylotypes assigned to Bacteroides, Clostridia_UCG-014, Clostridium Lachnospiraceae, Lachnospiraceae_NK4A136, and Parabacteroides were closely associated with the nonsevere cohort, among which, ASV8_Lachnospiraceae_NK4A136 was the most associated one.					
35127946	3	72	theme	rat	517:519	arg1	injury					527:532	D-GalN-induced rat liver injury	502:532	D-GalN-induced rat liver injury	502:532	The present study is aimed at determining the multiple intestinal bacteria associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	5	73	theme	MIP-3α	712:717	arg1	level					650:654	Higher level	643:654	Higher level of ALB	643:661	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	5	73	theme	MIP-3α	712:717	arg1	levels					673:678	lower levels	667:678	lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α	667:717	Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3α were determined in the nonsevere cohort than the severe cohort.					
35127946	11	74	theme	rat	1767:1769	arg1	injury					1777:1782	D-GalN-induced rat liver injury	1752:1782	D-GalN-induced rat liver injury	1752:1782	In conclusion, some intestinal bacteria, e.g., Lachnospiraceae_NK4A136, Parabacteroides, and Clostridium, were associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	6	75	theme	severe	958:963	arg1	cohorts					965:971	the nonsevere and severe cohorts	940:971	the nonsevere and severe cohorts	940:971	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	3	76	theme	intestinal	421:430	arg1	bacteria					432:439	the multiple intestinal bacteria	408:439	the multiple intestinal bacteria associated with the better protective effect of LI09 against D-GalN-induced rat liver injury	408:532	The present study is aimed at determining the multiple intestinal bacteria associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	4	77	theme	rat	539:541	arg1	cohorts					543:549	Two rat cohorts	535:549	Two rat cohorts	535:549	Two rat cohorts, i.e., the nonsevere and severe cohorts, were divided based on their liver injury severity.					
35127946	6	78	dep	Shannon	836:842	arg1	indices					856:862	indices	856:862	indices	856:862	The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts.					
35127946	11	79	theme	liver	1771:1775	arg1	injury					1777:1782	D-GalN-induced rat liver injury	1752:1782	D-GalN-induced rat liver injury	1752:1782	In conclusion, some intestinal bacteria, e.g., Lachnospiraceae_NK4A136, Parabacteroides, and Clostridium, were associated with the better protective effect of LI09 against D-GalN-induced rat liver injury.					
35127946	4	80	theme	injury	626:631	arg1	severity					633:640	their liver injury severity	614:640	their liver injury severity	614:640	Two rat cohorts, i.e., the nonsevere and severe cohorts, were divided based on their liver injury severity.					
35121528	4	0	theme	elution	804:810	arg1	conditions					812:821	elution conditions	804:821	elution conditions	804:821	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	4	1	theme	infectious	943:952	arg1	viruses					954:960	pure infectious viruses	938:960	pure infectious viruses	938:960	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	4	2	theme	sample	750:755	arg1	composition					762:772	sample feed composition	750:772	sample feed composition	750:772	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	8	3	theme	nanoparticles	1672:1684	arg1	purification					1636:1647	purification	1636:1647	purification of various viruses and nanoparticles, including adenovirus	1636:1706	This is especially interesting as the method has high potential to be used for purification of various viruses and nanoparticles, including adenovirus.					
35121528	6	4	theme	viruses	1347:1353	arg1	%					1380:1380	54%	1378:1380	54%	1378:1380	The yield of infectious viruses of the lysate feed was 54%.					
35121528	6	4	theme	viruses	1347:1353	arg1	yield					1327:1331	The yield	1323:1331	The yield of infectious viruses of the lysate feed	1323:1372	The yield of infectious viruses of the lysate feed was 54%.					
35121528	5	5	theme	3.5-fold	1151:1158	arg1	increase					1166:1173	a 3.5-fold yield increase	1149:1173	a 3.5-fold yield increase as compared to the conventional method	1149:1212	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	7	6	theme	methods	1428:1434	arg1	scalability					1392:1402	The easy scalability	1383:1402	The easy scalability of chromatography-based methods	1383:1434	The easy scalability of chromatography-based methods provide a direct route to industrial usage without any significant changes needed to be made to the purification regime.					
35121528	8	7	theme	high	1606:1609	arg1	potential					1611:1619	high potential	1606:1619	high potential	1606:1619	This is especially interesting as the method has high potential to be used for purification of various viruses and nanoparticles, including adenovirus.					
35121528	7	8	theme	purification	1536:1547	arg1	regime					1549:1554	the purification regime	1532:1554	the purification regime	1532:1554	The easy scalability of chromatography-based methods provide a direct route to industrial usage without any significant changes needed to be made to the purification regime.					
35121528	3	9	theme	sample	675:680	arg1	transfer					659:666	no physical transfer	647:666	no physical transfer of the sample	647:680	This inline approach helps to minimize the loss of virus in the process and streamlines time consumption, since no physical transfer of the sample is required between purification steps.					
35121528	5	10	dep	7 × 1012	1294:1301	arg1	to					1291:1292	to	1291:1292	to	1291:1292	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	2	11	theme	density	345:351	arg1	ultracentrifugation					362:380	density gradient ultracentrifugation	345:380	density gradient ultracentrifugation	345:380	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	2	12	theme	gradient	353:360	arg1	ultracentrifugation					362:380	density gradient ultracentrifugation	345:380	density gradient ultracentrifugation	345:380	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	3	13	theme	time	623:626	arg1	consumption					628:638	streamlines time consumption	611:638	streamlines time consumption	611:638	This inline approach helps to minimize the loss of virus in the process and streamlines time consumption, since no physical transfer of the sample is required between purification steps.					
35121528	7	14	theme	industrial	1462:1471	arg1	usage					1473:1477	industrial usage	1462:1477	industrial usage	1462:1477	The easy scalability of chromatography-based methods provide a direct route to industrial usage without any significant changes needed to be made to the purification regime.					
35121528	4	15	theme	maximal	921:927	arg1	yield					929:933	maximal yield	921:933	maximal yield of pure infectious viruses	921:960	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	3	16	from	loss	578:581	arg1	process					599:605	the process	595:605	the process	595:605	This inline approach helps to minimize the loss of virus in the process and streamlines time consumption, since no physical transfer of the sample is required between purification steps.					
35121528	7	17	theme	direct	1446:1451	arg1	route					1453:1457	a direct route	1444:1457	a direct route to industrial usage without any significant changes needed to be made to the purification regime	1444:1554	The easy scalability of chromatography-based methods provide a direct route to industrial usage without any significant changes needed to be made to the purification regime.					
35121528	5	18	theme	high	1240:1243	arg1	infectivity					1254:1264	the high specific infectivity	1236:1264	the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein)	1236:1320	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	6	19	theme	lysate	1362:1367	arg1	feed					1369:1372	the lysate feed	1358:1372	the lysate feed	1358:1372	The yield of infectious viruses of the lysate feed was 54%.					
35121528	0	20	from	lysate	48:53	arg1	purification					14:25	Inline-tandem purification	0:25	Inline-tandem purification of viruses from cell lysate by agarose-based chromatography	0:85	Inline-tandem purification of viruses from cell lysate by agarose-based chromatography.					
35121528	7	21	theme	chromatography-based	1407:1426	arg1	methods					1428:1434	chromatography-based methods	1407:1434	chromatography-based methods	1407:1434	The easy scalability of chromatography-based methods provide a direct route to industrial usage without any significant changes needed to be made to the purification regime.					
35121528	2	22	theme	precipitation	327:339	arg1	steps					382:386	relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps	280:386	relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps	280:386	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	1	23	theme	non-pathogenic	189:202	arg1	PRD1					221:224	the non-pathogenic infectious virus PRD1	185:224	the non-pathogenic infectious virus PRD1	185:224	An efficient chromatography-based virus purification method has been developed and validated for the non-pathogenic infectious virus PRD1.					
35121528	5	24	with	320 min	1055:1061	arg1	yield					1076:1080	a total yield	1068:1080	a total yield of 13 mg purified particles per litre of cell lysate	1068:1133	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	5	25	theme	lysate	1128:1133	arg1	litre					1114:1118	litre	1114:1118	litre of cell lysate	1114:1133	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	4	26	theme	SEC	884:886	arg1	resins					888:893	SEC resins	884:893	SEC resins	884:893	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	2	27	theme	labour-intensive	310:325	arg1	precipitation					327:339	relatively time-consuming and labour-intensive precipitation	280:339	relatively time-consuming and labour-intensive precipitation	280:339	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	1	28	theme	infectious	204:213	arg1	PRD1					221:224	the non-pathogenic infectious virus PRD1	185:224	the non-pathogenic infectious virus PRD1	185:224	An efficient chromatography-based virus purification method has been developed and validated for the non-pathogenic infectious virus PRD1.					
35121528	1	29	theme	efficient	91:99	arg1	method					141:146	An efficient chromatography-based virus purification method	88:146	An efficient chromatography-based virus purification method	88:146	An efficient chromatography-based virus purification method has been developed and validated for the non-pathogenic infectious virus PRD1.					
35121528	5	30	theme	cell	1123:1126	arg1	lysate					1128:1133	cell lysate	1123:1133	cell lysate	1123:1133	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	1	31	theme	virus	215:219	arg1	PRD1					221:224	the non-pathogenic infectious virus PRD1	185:224	the non-pathogenic infectious virus PRD1	185:224	An efficient chromatography-based virus purification method has been developed and validated for the non-pathogenic infectious virus PRD1.					
35121528	0	32	theme	Inline-tandem	0:12	arg1	purification					14:25	Inline-tandem purification	0:25	Inline-tandem purification of viruses from cell lysate by agarose-based chromatography	0:85	Inline-tandem purification of viruses from cell lysate by agarose-based chromatography.					
35121528	3	33	theme	streamlines	611:621	arg1	consumption					628:638	streamlines time consumption	611:638	streamlines time consumption	611:638	This inline approach helps to minimize the loss of virus in the process and streamlines time consumption, since no physical transfer of the sample is required between purification steps.					
35121528	5	34	dep	product	1273:1279	arg1	pfu/mg					1303:1308	6 × 1012 to 7 × 1012 pfu/mg	1282:1308	6 × 1012 to 7 × 1012 pfu/mg of protein	1282:1319	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	1	35	theme	chromatography-based	101:120	arg1	method					141:146	An efficient chromatography-based virus purification method	88:146	An efficient chromatography-based virus purification method	88:146	An efficient chromatography-based virus purification method has been developed and validated for the non-pathogenic infectious virus PRD1.					
35121528	2	36	theme	single	433:438	arg1	flow					440:443	a single flow	431:443	a single flow	431:443	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	0	37	theme	viruses	30:36	arg1	purification					14:25	Inline-tandem purification	0:25	Inline-tandem purification of viruses from cell lysate by agarose-based chromatography	0:85	Inline-tandem purification of viruses from cell lysate by agarose-based chromatography.					
35121528	4	38	theme	binding	783:789	arg1	capacity					791:798	dynamic binding capacity	775:798	dynamic binding capacity	775:798	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	2	39	theme	ultracentrifugation	362:380	arg1	steps					382:386	relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps	280:386	relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps	280:386	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	2	40	theme	size	485:488	arg1	chromatography					500:513	tandem-coupled anion exchange and size exclusion chromatography	451:513	chromatography	500:513	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	2	41	theme	exclusion	490:498	arg1	chromatography					500:513	tandem-coupled anion exchange and size exclusion chromatography	451:513	chromatography	500:513	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	3	42	theme	virus	586:590	arg1	loss					578:581	the loss	574:581	the loss of virus in the process	574:605	This inline approach helps to minimize the loss of virus in the process and streamlines time consumption, since no physical transfer of the sample is required between purification steps.					
35121528	3	42	theme	virus	586:590	arg1	consumption					628:638	streamlines time consumption	611:638	streamlines time consumption	611:638	This inline approach helps to minimize the loss of virus in the process and streamlines time consumption, since no physical transfer of the sample is required between purification steps.					
35121528	5	43	theme	purified	1091:1098	arg1	particles					1100:1108	13 mg purified particles	1085:1108	13 mg purified particles	1085:1108	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	1	44	theme	purification	128:139	arg1	method					141:146	An efficient chromatography-based virus purification method	88:146	An efficient chromatography-based virus purification method	88:146	An efficient chromatography-based virus purification method has been developed and validated for the non-pathogenic infectious virus PRD1.					
35121528	0	45	theme	cell	43:46	arg1	lysate					48:53	cell lysate	43:53	cell lysate	43:53	Inline-tandem purification of viruses from cell lysate by agarose-based chromatography.					
35121528	5	46	theme	virus	1007:1011	arg1	sample					1013:1018	a high-quality virus sample	992:1018	a high-quality virus sample	992:1018	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	6	47	theme	infectious	1336:1345	arg1	viruses					1347:1353	infectious viruses	1336:1353	infectious viruses of the lysate feed	1336:1372	The yield of infectious viruses of the lysate feed was 54%.					
35121528	3	48	theme	purification	702:713	arg1	steps					715:719	purification steps	702:719	purification steps	702:719	This inline approach helps to minimize the loss of virus in the process and streamlines time consumption, since no physical transfer of the sample is required between purification steps.					
35121528	4	49	theme	exclusion	863:871	arg1	limits					873:878	different exclusion limits	853:878	the AIEX resin as well as different exclusion limits for SEC resins	827:893	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	5	50	theme	protein	1313:1319	arg1	pfu/mg					1303:1308	6 × 1012 to 7 × 1012 pfu/mg	1282:1308	6 × 1012 to 7 × 1012 pfu/mg of protein	1282:1319	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	2	51	theme	exchange	472:479	arg1	columns					526:532	tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns	451:532	tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns	451:532	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	2	52	theme	tandem-coupled	451:464	arg1	exchange					472:479	tandem-coupled anion exchange and size exclusion chromatography	451:513	exchange	472:479	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	2	52	theme	tandem-coupled	451:464	arg1	AIEX-SEC					516:523	AIEX-SEC	516:523	AIEX-SEC	516:523	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	7	53	theme	easy	1387:1390	arg1	scalability					1392:1402	The easy scalability	1383:1402	The easy scalability of chromatography-based methods	1383:1434	The easy scalability of chromatography-based methods provide a direct route to industrial usage without any significant changes needed to be made to the purification regime.					
35121528	2	54	theme	chromatography	500:513	arg1	columns					526:532	tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns	451:532	tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns	451:532	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	2	55	theme	anion	466:470	arg1	exchange					472:479	tandem-coupled anion exchange and size exclusion chromatography	451:513	exchange	472:479	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	2	55	theme	anion	466:470	arg1	AIEX-SEC					516:523	AIEX-SEC	516:523	AIEX-SEC	516:523	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	4	56	theme	pure	938:941	arg1	viruses					954:960	pure infectious viruses	938:960	pure infectious viruses	938:960	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	5	57	theme	conventional	1194:1205	arg1	method					1207:1212	the conventional method	1190:1212	the conventional method	1190:1212	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	5	58	dep	increase	1166:1173	arg1	compared					1178:1185	compared	1178:1185	compared to the conventional method	1178:1212	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	5	59	theme	yield	1160:1164	arg1	increase					1166:1173	a 3.5-fold yield increase	1149:1173	a 3.5-fold yield increase as compared to the conventional method	1149:1212	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	5	60	theme	total	1070:1074	arg1	yield					1076:1080	a total yield	1068:1080	a total yield of 13 mg purified particles per litre of cell lysate	1068:1133	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	2	61	theme	conventional	243:254	arg1	method					256:261	the conventional method	239:261	the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps	239:386	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	4	62	theme	feed	757:760	arg1	composition					762:772	sample feed composition	750:772	sample feed composition	750:772	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	8	63	theme	various	1652:1658	arg1	viruses					1660:1666	viruses	1660:1666	viruses	1660:1666	This is especially interesting as the method has high potential to be used for purification of various viruses and nanoparticles, including adenovirus.					
35121528	8	63	theme	various	1652:1658	arg1	adenovirus					1697:1706	adenovirus	1697:1706	adenovirus	1697:1706	This is especially interesting as the method has high potential to be used for purification of various viruses and nanoparticles, including adenovirus.					
35121528	4	64	theme	different	853:861	arg1	limits					873:878	different exclusion limits	853:878	the AIEX resin as well as different exclusion limits for SEC resins	827:893	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	4	65	theme	viruses	954:960	arg1	yield					929:933	maximal yield	921:933	maximal yield of pure infectious viruses	921:960	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	8	66	theme	viruses	1660:1666	arg1	purification					1636:1647	purification	1636:1647	purification of various viruses and nanoparticles, including adenovirus	1636:1706	This is especially interesting as the method has high potential to be used for purification of various viruses and nanoparticles, including adenovirus.					
35121528	3	67	theme	physical	650:657	arg1	transfer					659:666	no physical transfer	647:666	no physical transfer of the sample	647:680	This inline approach helps to minimize the loss of virus in the process and streamlines time consumption, since no physical transfer of the sample is required between purification steps.					
35121528	3	68	theme	inline	540:545	arg1	approach					547:554	This inline approach	535:554	This inline approach	535:554	This inline approach helps to minimize the loss of virus in the process and streamlines time consumption, since no physical transfer of the sample is required between purification steps.					
35121528	1	69	theme	virus	122:126	arg1	method					141:146	An efficient chromatography-based virus purification method	88:146	An efficient chromatography-based virus purification method	88:146	An efficient chromatography-based virus purification method has been developed and validated for the non-pathogenic infectious virus PRD1.					
35121528	5	70	theme	specific	1245:1252	arg1	infectivity					1254:1264	the high specific infectivity	1236:1264	the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein)	1236:1320	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	4	71	theme	AIEX	831:834	arg1	resin					836:840	the AIEX resin	827:840	the AIEX resin as well as different exclusion limits for SEC resins	827:893	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	2	72	theme	time-consuming	291:304	arg1	precipitation					327:339	relatively time-consuming and labour-intensive precipitation	280:339	relatively time-consuming and labour-intensive precipitation	280:339	Compared to the conventional method that consists of relatively time-consuming and labour-intensive precipitation and density gradient ultracentrifugation steps, the method developed here is performed in a single flow using tandem-coupled anion exchange and size exclusion chromatography (AIEX-SEC) columns.					
35121528	5	73	theme	particles	1100:1108	arg1	yield					1076:1080	a total yield	1068:1080	a total yield of 13 mg purified particles per litre of cell lysate	1068:1133	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	5	74	theme	new	978:980	arg1	approach					982:989	this new approach	973:989	this new approach	973:989	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	4	75	theme	dynamic	775:781	arg1	capacity					791:798	dynamic binding capacity	775:798	dynamic binding capacity	775:798	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	5	76	theme	13 mg	1085:1089	arg1	particles					1100:1108	13 mg purified particles	1085:1108	13 mg purified particles	1085:1108	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	8	77	contain	has	1602:1604	arg2	potential					1611:1619	high potential	1606:1619	high potential	1606:1619	This is especially interesting as the method has high potential to be used for purification of various viruses and nanoparticles, including adenovirus.					
35121528	8	77	contain	has	1602:1604	arg1	method					1595:1600	the method	1591:1600	the method	1591:1600	This is especially interesting as the method has high potential to be used for purification of various viruses and nanoparticles, including adenovirus.					
35121528	5	78	theme	high-quality	994:1005	arg1	sample					1013:1018	a high-quality virus sample	992:1018	a high-quality virus sample	992:1018	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	3	79	from	consumption	628:638	arg1	process					599:605	the process	595:605	the process	595:605	This inline approach helps to minimize the loss of virus in the process and streamlines time consumption, since no physical transfer of the sample is required between purification steps.					
35121528	0	80	theme	agarose-based	58:70	arg1	chromatography					72:85	agarose-based chromatography	58:85	agarose-based chromatography	58:85	Inline-tandem purification of viruses from cell lysate by agarose-based chromatography.					
35121528	7	81	theme	significant	1491:1501	arg1	changes					1503:1509	any significant changes	1487:1509	any significant changes needed to be made to the purification regime	1487:1554	The easy scalability of chromatography-based methods provide a direct route to industrial usage without any significant changes needed to be made to the purification regime.					
35121528	4	82	theme	development	729:739	arg1	process					741:747	the development process	725:747	the development process	725:747	In the development process, sample feed composition, dynamic binding capacity and elution conditions for the AIEX resin as well as different exclusion limits for SEC resins were optimized to achieve maximal yield of pure infectious viruses.					
35121528	5	83	theme	product	1273:1279	arg1	infectivity					1254:1264	the high specific infectivity	1236:1264	the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein)	1236:1320	Utilizing this new approach, a high-quality virus sample was produced from a lysate feed in 320 min with a total yield of 13 mg purified particles per litre of cell lysate, constituting a 3.5-fold yield increase as compared to the conventional method, without compromising the high specific infectivity of the product (6 × 1012 to 7 × 1012 pfu/mg of protein).					
35121528	6	84	theme	feed	1369:1372	arg1	viruses					1347:1353	infectious viruses	1336:1353	infectious viruses of the lysate feed	1336:1372	The yield of infectious viruses of the lysate feed was 54%.					
36522831	4	0	theme	sequencing	668:677	arg1	approaches					679:688	biochemical and high-throughput sequencing approaches	636:688	biochemical and high-throughput sequencing approaches	636:688	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	4	1	theme	recognition	550:560	arg1	receptor					562:569	a secreted pattern recognition receptor	531:569	a secreted pattern recognition receptor (PRR)	531:575	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	4	1	theme	recognition	550:560	arg1	domestica					500:508	Musca domestica PGRP-SC (MdPGRP-SC)	494:528	Musca domestica PGRP-SC (MdPGRP-SC)	494:528	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	4	1	theme	recognition	550:560	arg1	PRR					572:574	PRR	572:574	PRR	572:574	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	5	2	theme	various	750:756	arg1	patterns					788:795	various pathogen-associated molecular patterns	750:795	various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose	750:876	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	2	theme	various	750:756	arg1	D-galactose					866:876	D-galactose	866:876	D-galactose	866:876	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	2	theme	various	750:756	arg1	lipopolysaccharide					836:853	lipopolysaccharide	836:853	lipopolysaccharide (LPS)	836:859	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	2	theme	various	750:756	arg1	PAMPs					798:802	PAMPs	798:802	PAMPs	798:802	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	2	theme	various	750:756	arg1	peptidoglycan					815:827	peptidoglycan	815:827	peptidoglycan (PGN)	815:833	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	4	3	theme	Musca	494:498	arg1	receptor					562:569	a secreted pattern recognition receptor	531:569	a secreted pattern recognition receptor (PRR)	531:575	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	4	3	theme	Musca	494:498	arg1	domestica					500:508	Musca domestica PGRP-SC (MdPGRP-SC)	494:528	Musca domestica PGRP-SC (MdPGRP-SC)	494:528	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	11	4	from	conservation	1832:1843	arg1	insects					1867:1873	insects	1867:1873	insects	1867:1873	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	2	5	theme	beneficial	327:336	arg1	interactions					338:349	mutually beneficial interactions	318:349	mutually beneficial interactions with the gut microbiota	318:373	The hosts establish mutually beneficial interactions with the gut microbiota.					
36522831	1	6	theme	microorganisms	225:238	arg1	array					216:220	a remarkable array	203:220	a remarkable array of microorganisms which are referred to collectively as the gut microbiota	203:295	The gastrointestinal tract of all animals, including insects, are colonized by a remarkable array of microorganisms which are referred to collectively as the gut microbiota.					
36522831	4	7	dep	domestica	500:508	arg1	MdPGRP-SC					519:527	MdPGRP-SC	519:527	MdPGRP-SC	519:527	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	4	7	dep	domestica	500:508	arg1	PGRP-SC					510:516	PGRP-SC	510:516	Musca domestica PGRP-SC (MdPGRP-SC)	494:528	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	10	8	theme	degrading	1708:1716	arg1	PAMPs					1742:1746	degrading or neutralizing various PAMPs	1708:1746	degrading or neutralizing various PAMPs	1708:1746	We hypothesize that MdPGRP-SC is involved in maintaining gut homeostasis by modulating the immune intensity of the gut through multiple mechanisms, including degrading or neutralizing various PAMPs and selectively suppressing the growth of some bacteria.					
36522831	11	9	theme	candidate	1915:1923	arg1	targets					1925:1931	promising candidate targets	1905:1931	promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming	1905:2036	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	11	9	theme	candidate	1915:1923	arg1	PGRPs					1890:1894	the catalytic PGRPs	1876:1894	the catalytic PGRPs	1876:1894	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	11	10	theme	bacterial	2000:2008	arg1	infection					2010:2018	bacterial infection	2000:2018	bacterial infection in insect farming	2000:2036	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	11	11	theme	insect	2023:2028	arg1	farming					2030:2036	insect farming	2023:2036	insect farming	2023:2036	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	10	12	theme	neutralizing	1721:1732	arg1	PAMPs					1742:1746	degrading or neutralizing various PAMPs	1708:1746	degrading or neutralizing various PAMPs	1708:1746	We hypothesize that MdPGRP-SC is involved in maintaining gut homeostasis by modulating the immune intensity of the gut through multiple mechanisms, including degrading or neutralizing various PAMPs and selectively suppressing the growth of some bacteria.					
36522831	11	13	theme	family	1857:1862	arg1	conservation					1832:1843	the functional conservation	1817:1843	the functional conservation of the PGRP family in insects	1817:1873	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	11	14	theme	infection	2010:2018	arg1	treatment					1987:1995	the treatment	1983:1995	the treatment of bacterial infection in insect farming	1983:2036	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	10	15	theme	multiple	1677:1684	arg1	mechanisms					1686:1695	multiple mechanisms	1677:1695	multiple mechanisms	1677:1695	We hypothesize that MdPGRP-SC is involved in maintaining gut homeostasis by modulating the immune intensity of the gut through multiple mechanisms, including degrading or neutralizing various PAMPs and selectively suppressing the growth of some bacteria.					
36522831	0	16	theme	house	97:101	arg1	domestica					113:121	the house fly Musca domestica	93:121	the house fly Musca domestica	93:121	PGRP-SC shapes gut microbiota richness, diversity, and composition by modulating immunity in the house fly Musca domestica.					
36522831	9	17	theme	Gram-negative	1526:1538	arg1	bacteria					1540:1547	Gram-negative bacteria	1526:1547	Gram-negative bacteria	1526:1547	In addition, depleted MdPGRP-SC also altered the gut microbiota composition and led to increased ratios of Gram-negative bacteria.					
36522831	10	18	theme	immune	1641:1646	arg1	intensity					1648:1656	the immune intensity	1637:1656	the immune intensity of the gut	1637:1667	We hypothesize that MdPGRP-SC is involved in maintaining gut homeostasis by modulating the immune intensity of the gut through multiple mechanisms, including degrading or neutralizing various PAMPs and selectively suppressing the growth of some bacteria.					
36522831	11	19	theme	promising	1905:1913	arg1	targets					1925:1931	promising candidate targets	1905:1931	promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming	1905:2036	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	11	19	theme	promising	1905:1913	arg1	PGRPs					1890:1894	the catalytic PGRPs	1876:1894	the catalytic PGRPs	1876:1894	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	9	20	theme	bacteria	1540:1547	arg1	ratios					1516:1521	increased ratios	1506:1521	increased ratios of Gram-negative bacteria	1506:1547	In addition, depleted MdPGRP-SC also altered the gut microbiota composition and led to increased ratios of Gram-negative bacteria.					
36522831	4	21	theme	community	607:615	arg1	structure					617:625	the gut microbial community structure	589:625	the gut microbial community structure	589:625	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	3	22	dep	mechanisms	389:398	arg1	shaping					400:406	shaping	400:406	shaping	400:406	However, the mechanisms shaping these interactions remain to be better understood.					
36522831	6	23	dep	bacteria	1037:1044	arg1	subtilis					1056:1063	Bacillus subtilis	1047:1063	Bacillus subtilis	1047:1063	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	6	23	dep	bacteria	1037:1044	arg1	aureus					1084:1089	Staphylococcus aureus	1069:1089	Staphylococcus aureus	1069:1089	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	11	24	theme	catalytic	1880:1888	arg1	targets					1925:1931	promising candidate targets	1905:1931	promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming	1905:2036	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	11	24	theme	catalytic	1880:1888	arg1	PGRPs					1890:1894	the catalytic PGRPs	1876:1894	the catalytic PGRPs	1876:1894	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	8	25	theme	coli	1343:1346	arg1	persistence					1316:1326	the persistence	1312:1326	the persistence of ingested E. coli	1312:1346	Knockdown of MdPGRP-SC by RNAi reduced the persistence of ingested E. coli and the load of indigenous microbiota in the larval gut significantly.					
36522831	8	25	theme	coli	1343:1346	arg1	load					1356:1359	the load	1352:1359	the load of indigenous microbiota	1352:1384	Knockdown of MdPGRP-SC by RNAi reduced the persistence of ingested E. coli and the load of indigenous microbiota in the larval gut significantly.					
36522831	2	26	theme	gut	360:362	arg1	microbiota					364:373	the gut microbiota	356:373	the gut microbiota	356:373	The hosts establish mutually beneficial interactions with the gut microbiota.					
36522831	0	27	theme	microbiota	19:28	arg1	richness					30:37	gut microbiota richness	15:37	gut microbiota richness	15:37	PGRP-SC shapes gut microbiota richness, diversity, and composition by modulating immunity in the house fly Musca domestica.					
36522831	8	28	theme	MdPGRP-SC	1286:1294	arg1	Knockdown					1273:1281	Knockdown	1273:1281	Knockdown of MdPGRP-SC by RNAi	1273:1302	Knockdown of MdPGRP-SC by RNAi reduced the persistence of ingested E. coli and the load of indigenous microbiota in the larval gut significantly.					
36522831	0	29	theme	Musca	107:111	arg1	domestica					113:121	the house fly Musca domestica	93:121	the house fly Musca domestica	93:121	PGRP-SC shapes gut microbiota richness, diversity, and composition by modulating immunity in the house fly Musca domestica.					
36522831	10	30	theme	bacteria	1795:1802	arg1	growth					1780:1785	the growth	1776:1785	the growth of some bacteria	1776:1802	We hypothesize that MdPGRP-SC is involved in maintaining gut homeostasis by modulating the immune intensity of the gut through multiple mechanisms, including degrading or neutralizing various PAMPs and selectively suppressing the growth of some bacteria.					
36522831	11	31	theme	vector	1955:1960	arg1	control					1962:1968	vector control	1955:1968	vector control	1955:1968	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	9	32	theme	depleted	1432:1439	arg1	MdPGRP-SC					1441:1449	depleted MdPGRP-SC	1432:1449	depleted MdPGRP-SC	1432:1449	In addition, depleted MdPGRP-SC also altered the gut microbiota composition and led to increased ratios of Gram-negative bacteria.					
36522831	0	33	theme	gut	15:17	arg1	richness					30:37	gut microbiota richness	15:37	gut microbiota richness	15:37	PGRP-SC shapes gut microbiota richness, diversity, and composition by modulating immunity in the house fly Musca domestica.					
36522831	4	34	theme	secreted	533:540	arg1	receptor					562:569	a secreted pattern recognition receptor	531:569	a secreted pattern recognition receptor (PRR)	531:575	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	4	34	theme	secreted	533:540	arg1	domestica					500:508	Musca domestica PGRP-SC (MdPGRP-SC)	494:528	Musca domestica PGRP-SC (MdPGRP-SC)	494:528	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	4	34	theme	secreted	533:540	arg1	PRR					572:574	PRR	572:574	PRR	572:574	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	0	35	theme	fly	103:105	arg1	domestica					113:121	the house fly Musca domestica	93:121	the house fly Musca domestica	93:121	PGRP-SC shapes gut microbiota richness, diversity, and composition by modulating immunity in the house fly Musca domestica.					
36522831	4	36	theme	high-throughput	652:666	arg1	approaches					679:688	biochemical and high-throughput sequencing approaches	636:688	biochemical and high-throughput sequencing approaches	636:688	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	9	37	theme	increased	1506:1514	arg1	ratios					1516:1521	increased ratios	1506:1521	increased ratios of Gram-negative bacteria	1506:1547	In addition, depleted MdPGRP-SC also altered the gut microbiota composition and led to increased ratios of Gram-negative bacteria.					
36522831	10	38	theme	gut	1665:1667	arg1	intensity					1648:1656	the immune intensity	1637:1656	the immune intensity of the gut	1637:1667	We hypothesize that MdPGRP-SC is involved in maintaining gut homeostasis by modulating the immune intensity of the gut through multiple mechanisms, including degrading or neutralizing various PAMPs and selectively suppressing the growth of some bacteria.					
36522831	5	39	theme	pathogen-associated	758:776	arg1	patterns					788:795	various pathogen-associated molecular patterns	750:795	various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose	750:876	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	39	theme	pathogen-associated	758:776	arg1	D-galactose					866:876	D-galactose	866:876	D-galactose	866:876	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	39	theme	pathogen-associated	758:776	arg1	lipopolysaccharide					836:853	lipopolysaccharide	836:853	lipopolysaccharide (LPS)	836:859	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	39	theme	pathogen-associated	758:776	arg1	PAMPs					798:802	PAMPs	798:802	PAMPs	798:802	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	39	theme	pathogen-associated	758:776	arg1	peptidoglycan					815:827	peptidoglycan	815:827	peptidoglycan (PGN)	815:833	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	9	40	theme	gut	1468:1470	arg1	composition					1483:1493	the gut microbiota composition	1464:1493	the gut microbiota composition	1464:1493	In addition, depleted MdPGRP-SC also altered the gut microbiota composition and led to increased ratios of Gram-negative bacteria.					
36522831	6	41	theme	microorganisms	997:1010	arg1	bacteria					1037:1044	gram-positive bacteria	1023:1044	gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus)	1023:1090	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	6	41	theme	microorganisms	997:1010	arg1	bacteria					1107:1114	gram-negative bacteria	1093:1114	gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa)	1093:1160	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	6	41	theme	microorganisms	997:1010	arg1	yeast					1167:1171	yeast	1167:1171	yeast (Pichia pastoris)	1167:1189	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	6	41	theme	microorganisms	997:1010	arg1	kinds					988:992	different kinds	978:992	different kinds	978:992	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	8	42	theme	indigenous	1364:1373	arg1	microbiota					1375:1384	indigenous microbiota	1364:1384	indigenous microbiota	1364:1384	Knockdown of MdPGRP-SC by RNAi reduced the persistence of ingested E. coli and the load of indigenous microbiota in the larval gut significantly.					
36522831	6	43	theme	gram-positive	1023:1035	arg1	bacteria					1037:1044	gram-positive bacteria	1023:1044	gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus)	1023:1090	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	4	44	theme	microbial	597:605	arg1	structure					617:625	the gut microbial community structure	589:625	the gut microbial community structure	589:625	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	5	45	theme	molecular	778:786	arg1	patterns					788:795	various pathogen-associated molecular patterns	750:795	various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose	750:876	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	45	theme	molecular	778:786	arg1	D-galactose					866:876	D-galactose	866:876	D-galactose	866:876	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	45	theme	molecular	778:786	arg1	lipopolysaccharide					836:853	lipopolysaccharide	836:853	lipopolysaccharide (LPS)	836:859	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	45	theme	molecular	778:786	arg1	PAMPs					798:802	PAMPs	798:802	PAMPs	798:802	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	45	theme	molecular	778:786	arg1	peptidoglycan					815:827	peptidoglycan	815:827	peptidoglycan (PGN)	815:833	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	4	46	theme	pattern	542:548	arg1	receptor					562:569	a secreted pattern recognition receptor	531:569	a secreted pattern recognition receptor (PRR)	531:575	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	4	46	theme	pattern	542:548	arg1	domestica					500:508	Musca domestica PGRP-SC (MdPGRP-SC)	494:528	Musca domestica PGRP-SC (MdPGRP-SC)	494:528	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	4	46	theme	pattern	542:548	arg1	PRR					572:574	PRR	572:574	PRR	572:574	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	4	47	theme	gut	593:595	arg1	structure					617:625	the gut microbial community structure	589:625	the gut microbial community structure	589:625	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	10	48	theme	gut	1607:1609	arg1	homeostasis					1611:1621	gut homeostasis	1607:1621	gut homeostasis	1607:1621	We hypothesize that MdPGRP-SC is involved in maintaining gut homeostasis by modulating the immune intensity of the gut through multiple mechanisms, including degrading or neutralizing various PAMPs and selectively suppressing the growth of some bacteria.					
36522831	4	49	theme	domestica	500:508	arg1	roles					485:489	the roles	481:489	the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR),	481:576	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	5	50	theme	recombinant	695:705	arg1	rMdPGRP-SC					718:727	rMdPGRP-SC	718:727	rMdPGRP-SC	718:727	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	5	50	theme	recombinant	695:705	arg1	MdPGRP-SC					707:715	The recombinant MdPGRP-SC	691:715	The recombinant MdPGRP-SC (rMdPGRP-SC)	691:728	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	11	51	theme	functional	1821:1830	arg1	conservation					1832:1843	the functional conservation	1817:1843	the functional conservation of the PGRP family in insects	1817:1873	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	9	52	theme	microbiota	1472:1481	arg1	composition					1483:1493	the gut microbiota composition	1464:1493	the gut microbiota composition	1464:1493	In addition, depleted MdPGRP-SC also altered the gut microbiota composition and led to increased ratios of Gram-negative bacteria.					
36522831	2	53	with	interactions	338:349	arg1	microbiota					364:373	the gut microbiota	356:373	the gut microbiota	356:373	The hosts establish mutually beneficial interactions with the gut microbiota.					
36522831	8	54	theme	microbiota	1375:1384	arg1	persistence					1316:1326	the persistence	1312:1326	the persistence of ingested E. coli	1312:1346	Knockdown of MdPGRP-SC by RNAi reduced the persistence of ingested E. coli and the load of indigenous microbiota in the larval gut significantly.					
36522831	8	54	theme	microbiota	1375:1384	arg1	load					1356:1359	the load	1352:1359	the load of indigenous microbiota	1352:1384	Knockdown of MdPGRP-SC by RNAi reduced the persistence of ingested E. coli and the load of indigenous microbiota in the larval gut significantly.					
36522831	6	55	theme	gram-negative	1093:1105	arg1	bacteria					1107:1114	gram-negative bacteria	1093:1114	gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa)	1093:1160	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	7	56	theme	antibacterial	1223:1235	arg1	activity					1237:1244	weak antibacterial activity	1218:1244	weak antibacterial activity against Bacillus subtilis	1218:1270	rMdPGRP-SC also exhibited weak antibacterial activity against Bacillus subtilis.					
36522831	8	57	theme	larval	1393:1398	arg1	gut					1400:1402	the larval gut	1389:1402	the larval gut	1389:1402	Knockdown of MdPGRP-SC by RNAi reduced the persistence of ingested E. coli and the load of indigenous microbiota in the larval gut significantly.					
36522831	6	58	theme	Pichia	1174:1179	arg1	yeast					1167:1171	yeast	1167:1171	yeast (Pichia pastoris)	1167:1189	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	6	58	theme	Pichia	1174:1179	arg1	pastoris					1181:1188	Pichia pastoris	1174:1188	Pichia pastoris	1174:1188	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	5	59	theme	mild	893:896	arg1	affinity					898:905	mild affinity	893:905	mild affinity to β-1, 3-glucan, and D-mannose	893:937	The recombinant MdPGRP-SC (rMdPGRP-SC) could strongly bind various pathogen-associated molecular patterns (PAMPs) including peptidoglycan (PGN), lipopolysaccharide (LPS), and D-galactose, and exhibited mild affinity to β-1, 3-glucan, and D-mannose.					
36522831	10	60	theme	various	1734:1740	arg1	PAMPs					1742:1746	degrading or neutralizing various PAMPs	1708:1746	degrading or neutralizing various PAMPs	1708:1746	We hypothesize that MdPGRP-SC is involved in maintaining gut homeostasis by modulating the immune intensity of the gut through multiple mechanisms, including degrading or neutralizing various PAMPs and selectively suppressing the growth of some bacteria.					
36522831	4	61	theme	biochemical	636:646	arg1	approaches					679:688	biochemical and high-throughput sequencing approaches	636:688	biochemical and high-throughput sequencing approaches	636:688	Here, we investigated the roles of Musca domestica PGRP-SC (MdPGRP-SC), a secreted pattern recognition receptor (PRR), in shaping the gut microbial community structure by using biochemical and high-throughput sequencing approaches.					
36522831	11	62	from	farming	2030:2036	arg1	treatment					1987:1995	the treatment	1983:1995	the treatment of bacterial infection in insect farming	1983:2036	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	6	63	theme	different	978:986	arg1	bacteria					1037:1044	gram-positive bacteria	1023:1044	gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus)	1023:1090	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	6	63	theme	different	978:986	arg1	bacteria					1107:1114	gram-negative bacteria	1093:1114	gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa)	1093:1160	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	6	63	theme	different	978:986	arg1	yeast					1167:1171	yeast	1167:1171	yeast (Pichia pastoris)	1167:1189	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	6	63	theme	different	978:986	arg1	kinds					988:992	different kinds	978:992	different kinds	978:992	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	11	64	from	infection	2010:2018	arg1	farming					2030:2036	insect farming	2023:2036	insect farming	2023:2036	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	6	65	dep	bacteria	1107:1114	arg1	coli					1129:1132	Escherichia coli	1117:1132	Escherichia coli	1117:1132	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	6	65	dep	bacteria	1107:1114	arg1	aeruginosa					1150:1159	Pseudomonas aeruginosa	1138:1159	Pseudomonas aeruginosa	1138:1159	Meanwhile, rMdPGRP-SC could also bind different kinds of microorganisms, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Pichia pastoris).					
36522831	11	66	from	treatment	1987:1995	arg1	farming					2030:2036	insect farming	2023:2036	insect farming	2023:2036	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	7	67	theme	weak	1218:1221	arg1	activity					1237:1244	weak antibacterial activity	1218:1244	weak antibacterial activity against Bacillus subtilis	1218:1270	rMdPGRP-SC also exhibited weak antibacterial activity against Bacillus subtilis.					
36522831	1	68	theme	animals	158:164	arg1	tract					145:149	The gastrointestinal tract	124:149	The gastrointestinal tract of all animals, including insects,	124:184	The gastrointestinal tract of all animals, including insects, are colonized by a remarkable array of microorganisms which are referred to collectively as the gut microbiota.					
36522831	1	69	theme	remarkable	205:214	arg1	array					216:220	a remarkable array	203:220	a remarkable array of microorganisms which are referred to collectively as the gut microbiota	203:295	The gastrointestinal tract of all animals, including insects, are colonized by a remarkable array of microorganisms which are referred to collectively as the gut microbiota.					
36522831	1	70	theme	gastrointestinal	128:143	arg1	tract					145:149	The gastrointestinal tract	124:149	The gastrointestinal tract of all animals, including insects,	124:184	The gastrointestinal tract of all animals, including insects, are colonized by a remarkable array of microorganisms which are referred to collectively as the gut microbiota.					
36522831	11	71	theme	PGRP	1852:1855	arg1	family					1857:1862	the PGRP family	1848:1862	the PGRP family	1848:1862	Considering the functional conservation of the PGRP family in insects, the catalytic PGRPs might be promising candidate targets not only for pest and vector control but also for the treatment of bacterial infection in insect farming.					
36522831	1	72	theme	gut	282:284	arg1	microorganisms					225:238	microorganisms	225:238	microorganisms which are referred to collectively as the gut microbiota	225:295	The gastrointestinal tract of all animals, including insects, are colonized by a remarkable array of microorganisms which are referred to collectively as the gut microbiota.					
36522831	1	72	theme	gut	282:284	arg1	microbiota					286:295	the gut microbiota	278:295	the gut microbiota	278:295	The gastrointestinal tract of all animals, including insects, are colonized by a remarkable array of microorganisms which are referred to collectively as the gut microbiota.					
36365536	1	0	theme	tear	149:152	arg1	lipids					193:198	lipids	193:198	lipids	193:198	The adsorption of tear film compositions such as proteins and lipids on the orthokeratology lenses often lead to infection or corneal damage.					
36365536	1	0	theme	tear	149:152	arg1	compositions					159:170	tear film compositions	149:170	tear film compositions such as proteins and lipids	149:198	The adsorption of tear film compositions such as proteins and lipids on the orthokeratology lenses often lead to infection or corneal damage.					
36365536	1	0	theme	tear	149:152	arg1	proteins					180:187	proteins	180:187	proteins	180:187	The adsorption of tear film compositions such as proteins and lipids on the orthokeratology lenses often lead to infection or corneal damage.					
36365536	6	1	dep	evidence	954:961	arg1	applied					991:997	applied	991:997	applied	991:997	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	5	2	theme	polysaccharides	718:732	arg1	distinct					761:768	distinct	761:768	distinct	761:768	Although the effect of polysaccharides on lysozyme adsorption was distinct depending on the concentrations of polysaccharides, the overall results demonstrated that polysaccharides could decrease protein deposition over time.					
36365536	5	2	theme	polysaccharides	718:732	arg1	effect					708:713	the effect	704:713	the effect of polysaccharides on lysozyme adsorption	704:755	Although the effect of polysaccharides on lysozyme adsorption was distinct depending on the concentrations of polysaccharides, the overall results demonstrated that polysaccharides could decrease protein deposition over time.					
36365536	3	3	theme	lysozyme	510:517	arg1	adsorption					483:492	daily adsorption	477:492	daily adsorption of cholesterol, lysozyme, and albumin	477:530	By measuring daily adsorption of cholesterol, lysozyme, and albumin, our results showed that polysaccharides could weakly prevent cholesterol adsorption.					
36365536	6	4	theme	corneal	1206:1212	arg1	damage					1214:1219	corneal damage	1206:1219	corneal damage	1206:1219	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	6	5	theme	in	945:946	arg1	evidence					954:961	an in vitro evidence	942:961	an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers	942:1252	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	1	6	from	adsorption	135:144	arg1	orthokeratology					207:221	the orthokeratology	203:221	the orthokeratology	203:221	The adsorption of tear film compositions such as proteins and lipids on the orthokeratology lenses often lead to infection or corneal damage.					
36365536	1	7	theme	corneal	257:263	arg1	damage					265:270	corneal damage	257:270	corneal damage	257:270	The adsorption of tear film compositions such as proteins and lipids on the orthokeratology lenses often lead to infection or corneal damage.					
36365536	3	8	theme	albumin	524:530	arg1	adsorption					483:492	daily adsorption	477:492	daily adsorption of cholesterol, lysozyme, and albumin	477:530	By measuring daily adsorption of cholesterol, lysozyme, and albumin, our results showed that polysaccharides could weakly prevent cholesterol adsorption.					
36365536	1	9	theme	compositions	159:170	arg1	adsorption					135:144	The adsorption	131:144	The adsorption of tear film compositions such as proteins and lipids on the orthokeratology	131:221	The adsorption of tear film compositions such as proteins and lipids on the orthokeratology lenses often lead to infection or corneal damage.					
36365536	6	10	theme	artificial	1057:1066	arg1	solutions					1073:1081	artificial tear solutions	1057:1081	artificial tear solutions	1057:1081	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	4	11	theme	albumin	665:671	arg1	deposition					673:682	albumin deposition	665:682	albumin deposition	665:682	In addition, polysaccharides could also reduce albumin deposition over time.					
36365536	2	12	theme	tear	449:452	arg1	solution					454:461	artificial tear solution	438:461	artificial tear solution	438:461	In order to investigate whether polysaccharides could prevent tear compositions from being adsorbed on the lens, alginic acid and lambda-carrageenan were added into artificial tear solution.					
36365536	6	13	theme	coating	1002:1008	arg1	materials					1010:1018	coating materials	1002:1018	coating materials on the lens	1002:1030	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	6	13	theme	coating	1002:1008	arg1	polysaccharides					968:982	polysaccharides	968:982	polysaccharides	968:982	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	2	14	theme	artificial	438:447	arg1	solution					454:461	artificial tear solution	438:461	artificial tear solution	438:461	In order to investigate whether polysaccharides could prevent tear compositions from being adsorbed on the lens, alginic acid and lambda-carrageenan were added into artificial tear solution.					
36365536	6	15	theme	reduced	1172:1178	arg1	incidence					1180:1188	a reduced incidence	1170:1188	a reduced incidence of infection or corneal damage for orthokeratology lens wearers	1170:1252	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	0	16	theme	Polysaccharides	14:28	arg1	Effect					4:9	The Effect	0:9	The Effect of Polysaccharides on Preventing Proteins and Cholesterol from Being Adsorbed on the Surface of Orthokeratology Lenses	0:128	The Effect of Polysaccharides on Preventing Proteins and Cholesterol from Being Adsorbed on the Surface of Orthokeratology Lenses.					
36365536	0	17	theme	Lenses	123:128	arg1	Surface					96:102	the Surface	92:102	the Surface of Orthokeratology Lenses	92:128	The Effect of Polysaccharides on Preventing Proteins and Cholesterol from Being Adsorbed on the Surface of Orthokeratology Lenses.					
36365536	0	18	theme	Orthokeratology	107:121	arg1	Lenses					123:128	Orthokeratology Lenses	107:128	Orthokeratology Lenses	107:128	The Effect of Polysaccharides on Preventing Proteins and Cholesterol from Being Adsorbed on the Surface of Orthokeratology Lenses.					
36365536	1	19	theme	film	154:157	arg1	lipids					193:198	lipids	193:198	lipids	193:198	The adsorption of tear film compositions such as proteins and lipids on the orthokeratology lenses often lead to infection or corneal damage.					
36365536	1	19	theme	film	154:157	arg1	compositions					159:170	tear film compositions	149:170	tear film compositions such as proteins and lipids	149:198	The adsorption of tear film compositions such as proteins and lipids on the orthokeratology lenses often lead to infection or corneal damage.					
36365536	1	19	theme	film	154:157	arg1	proteins					180:187	proteins	180:187	proteins	180:187	The adsorption of tear film compositions such as proteins and lipids on the orthokeratology lenses often lead to infection or corneal damage.					
36365536	6	20	theme	eyedrops	1086:1093	arg1	composition					1042:1052	the composition	1038:1052	the composition of artificial tear solutions or eyedrops	1038:1093	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	6	20	theme	eyedrops	1086:1093	arg1	polysaccharides					968:982	polysaccharides	968:982	polysaccharides	968:982	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	5	21	theme	polysaccharides	805:819	arg1	concentrations					787:800	the concentrations	783:800	the concentrations of polysaccharides	783:819	Although the effect of polysaccharides on lysozyme adsorption was distinct depending on the concentrations of polysaccharides, the overall results demonstrated that polysaccharides could decrease protein deposition over time.					
36365536	2	22	theme	tear	335:338	arg1	compositions					340:351	tear compositions	335:351	tear compositions	335:351	In order to investigate whether polysaccharides could prevent tear compositions from being adsorbed on the lens, alginic acid and lambda-carrageenan were added into artificial tear solution.					
36365536	3	23	theme	cholesterol	594:604	arg1	adsorption					606:615	cholesterol adsorption	594:615	cholesterol adsorption	594:615	By measuring daily adsorption of cholesterol, lysozyme, and albumin, our results showed that polysaccharides could weakly prevent cholesterol adsorption.					
36365536	5	24	theme	protein	891:897	arg1	deposition					899:908	protein deposition	891:908	protein deposition	891:908	Although the effect of polysaccharides on lysozyme adsorption was distinct depending on the concentrations of polysaccharides, the overall results demonstrated that polysaccharides could decrease protein deposition over time.					
36365536	2	25	theme	alginic	386:392	arg1	acid					394:397	alginic acid	386:397	alginic acid	386:397	In order to investigate whether polysaccharides could prevent tear compositions from being adsorbed on the lens, alginic acid and lambda-carrageenan were added into artificial tear solution.					
36365536	5	26	theme	lysozyme	737:744	arg1	adsorption					746:755	lysozyme adsorption	737:755	lysozyme adsorption	737:755	Although the effect of polysaccharides on lysozyme adsorption was distinct depending on the concentrations of polysaccharides, the overall results demonstrated that polysaccharides could decrease protein deposition over time.					
36365536	6	27	theme	solutions	1073:1081	arg1	composition					1042:1052	the composition	1038:1052	the composition of artificial tear solutions or eyedrops	1038:1093	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	6	27	theme	solutions	1073:1081	arg1	polysaccharides					968:982	polysaccharides	968:982	polysaccharides	968:982	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	1	28	dep	lenses	223:228	arg1	lead					236:239	lead	236:239	lenses often lead to infection or corneal damage	223:270	The adsorption of tear film compositions such as proteins and lipids on the orthokeratology lenses often lead to infection or corneal damage.					
36365536	5	29	theme	overall	826:832	arg1	results					834:840	the overall results	822:840	the overall results	822:840	Although the effect of polysaccharides on lysozyme adsorption was distinct depending on the concentrations of polysaccharides, the overall results demonstrated that polysaccharides could decrease protein deposition over time.					
36365536	6	30	from	materials	1010:1018	arg1	lens					1027:1030	the lens	1023:1030	the lens	1023:1030	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	3	31	theme	cholesterol	497:507	arg1	adsorption					483:492	daily adsorption	477:492	daily adsorption of cholesterol, lysozyme, and albumin	477:530	By measuring daily adsorption of cholesterol, lysozyme, and albumin, our results showed that polysaccharides could weakly prevent cholesterol adsorption.					
36365536	6	32	theme	lens	1241:1244	arg1	wearers					1246:1252	orthokeratology lens wearers	1225:1252	orthokeratology lens wearers	1225:1252	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	3	33	theme	daily	477:481	arg1	adsorption					483:492	daily adsorption	477:492	daily adsorption of cholesterol, lysozyme, and albumin	477:530	By measuring daily adsorption of cholesterol, lysozyme, and albumin, our results showed that polysaccharides could weakly prevent cholesterol adsorption.					
36365536	6	34	theme	tear	1068:1071	arg1	solutions					1073:1081	artificial tear solutions	1057:1081	artificial tear solutions	1057:1081	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	6	35	theme	orthokeratology	1225:1239	arg1	wearers					1246:1252	orthokeratology lens wearers	1225:1252	orthokeratology lens wearers	1225:1252	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	6	36	theme	compositions	1140:1151	arg1	adsorption					1116:1125	adsorption	1116:1125	adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers	1116:1252	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	5	37	from	effect	708:713	arg1	adsorption					746:755	lysozyme adsorption	737:755	lysozyme adsorption	737:755	Although the effect of polysaccharides on lysozyme adsorption was distinct depending on the concentrations of polysaccharides, the overall results demonstrated that polysaccharides could decrease protein deposition over time.					
36365536	6	38	theme	film	1135:1138	arg1	compositions					1140:1151	tear film compositions	1130:1151	tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers	1130:1252	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	6	39	theme	infection	1193:1201	arg1	incidence					1180:1188	a reduced incidence	1170:1188	a reduced incidence of infection or corneal damage for orthokeratology lens wearers	1170:1252	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	6	40	dep	in	945:946	arg1	vitro					948:952	vitro	948:952	vitro	948:952	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	6	41	theme	damage	1214:1219	arg1	incidence					1180:1188	a reduced incidence	1170:1188	a reduced incidence of infection or corneal damage for orthokeratology lens wearers	1170:1252	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
36365536	6	42	theme	tear	1130:1133	arg1	film					1135:1138	tear film	1130:1138	tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers	1130:1252	Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.					
35123746	0	0	theme	rot	87:89	arg1	nanocomposites					21:34	Novel chitosan-based nanocomposites	0:34	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.	0:140	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	0	theme	rot	87:89	arg1	inhibition					91:100	root rot inhibition	82:100	root rot inhibition	82:100	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	8	1	theme	from	637:640	arg1	CS					642:643	CS	642:643	CS	642:643	from CS and CS/Mt obeyed well zero-order equation.					
35123746	12	2	theme	lower	1033:1037	arg1	concentrations					1039:1052	lower concentrations	1033:1052	lower concentrations	1033:1052	, have a strong inhibitory effect on the linear growth of the target pathogens, even at lower concentrations.					
35123746	5	3	theme	composites	545:554	arg1	morphology					531:540	morphology	531:540	morphology	531:540	The structure and morphology of composites were characterized by FTIR, XRD, SEM and TEM.					
35123746	5	3	theme	composites	545:554	arg1	structure					517:525	structure	517:525	structure	517:525	The structure and morphology of composites were characterized by FTIR, XRD, SEM and TEM.					
35123746	4	4	with	montmorillonite	483:497	arg1	CS/PANI					438:444	CS/PANI	438:444	CS/PANI	438:444	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	4	4	with	montmorillonite	483:497	arg1	polyaniline					425:435	polyaniline	425:435	polyaniline (CS/PANI)	425:445	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	0	5	theme	root	82:85	arg1	rot					87:89	root rot	82:89	root rot inhibition	82:100	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	14	6	theme	untreated-infected	1300:1317	arg1	controls					1319:1326	untreated-infected controls	1300:1326	untreated-infected controls	1300:1326	reduced both disease index and disease incidence parameters of both pathogens and possessed seedlings growth promoting potential of tomato compared to untreated-infected controls.					
35123746	14	7	theme	tomato	1281:1286	arg1	potential					1268:1276	potential	1268:1276	potential of tomato compared to untreated-infected controls	1268:1326	reduced both disease index and disease incidence parameters of both pathogens and possessed seedlings growth promoting potential of tomato compared to untreated-infected controls.					
35123746	9	8	theme	release	749:755	arg1	data					757:760	the release data	745:760	the release data from CS/PANI and CS/Mt composites	745:794	However, Higuchi and Korsmeyer-Peppas models fitted well the release data from CS/PANI and CS/Mt composites.					
35123746	14	9	theme	disease	1162:1168	arg1	index					1170:1174	disease index	1162:1174	disease index	1162:1174	reduced both disease index and disease incidence parameters of both pathogens and possessed seedlings growth promoting potential of tomato compared to untreated-infected controls.					
35123746	14	10	theme	pathogens	1217:1225	arg1	index					1170:1174	disease index	1162:1174	disease index	1162:1174	reduced both disease index and disease incidence parameters of both pathogens and possessed seedlings growth promoting potential of tomato compared to untreated-infected controls.					
35123746	14	10	theme	pathogens	1217:1225	arg1	parameters					1198:1207	disease incidence parameters	1180:1207	disease incidence parameters	1180:1207	reduced both disease index and disease incidence parameters of both pathogens and possessed seedlings growth promoting potential of tomato compared to untreated-infected controls.					
35123746	11	11	dep	In	887:888	arg1	vitro					890:894	vitro	890:894	vitro	890:894	In vitro assay, CS, Cinn., Van., CS/PANI and CS/PANI/Cinn.					
35123746	14	12	theme	disease	1180:1186	arg1	parameters					1198:1207	disease incidence parameters	1180:1207	disease incidence parameters	1180:1207	reduced both disease index and disease incidence parameters of both pathogens and possessed seedlings growth promoting potential of tomato compared to untreated-infected controls.					
35123746	5	13	dep	structure	517:525	arg1	The					513:515	The	513:515	The	513:515	The structure and morphology of composites were characterized by FTIR, XRD, SEM and TEM.					
35123746	10	14	theme	Fusarium	844:851	arg1	oxysporum					853:861	Fusarium oxysporum	844:861	Fusarium oxysporum	844:861	Their antifungal activity was examined towards Fusarium oxysporum and Pythium debaryanum.					
35123746	4	15	theme	chitosan/polyaniline/exfoliated	451:481	arg1	CS/PANI/Mt					500:509	CS/PANI/Mt	500:509	CS/PANI/Mt	500:509	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	4	15	theme	chitosan/polyaniline/exfoliated	451:481	arg1	montmorillonite					483:497	chitosan/polyaniline/exfoliated montmorillonite	451:497	chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt)	451:510	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	12	16	theme	pathogens	1014:1022	arg1	growth					993:998	the linear growth	982:998	the linear growth of the target pathogens	982:1022	, have a strong inhibitory effect on the linear growth of the target pathogens, even at lower concentrations.					
35123746	10	17	theme	antifungal	803:812	arg1	activity					814:821	Their antifungal activity	797:821	Their antifungal activity	797:821	Their antifungal activity was examined towards Fusarium oxysporum and Pythium debaryanum.					
35123746	0	18	theme	growth	106:111	arg1	nanocomposites					21:34	Novel chitosan-based nanocomposites	0:34	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.	0:140	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	18	theme	growth	106:111	arg1	management					113:122	growth management	106:122	growth management of tomato plants	106:139	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	12	19	theme	target	1007:1012	arg1	pathogens					1014:1022	the target pathogens	1003:1022	the target pathogens	1003:1022	, have a strong inhibitory effect on the linear growth of the target pathogens, even at lower concentrations.					
35123746	4	20	theme	sodium	362:367	arg1	montmorillonite					369:383	sodium montmorillonite	362:383	sodium montmorillonite (CS/Mt)	362:391	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	4	20	theme	sodium	362:367	arg1	CS/Mt					386:390	CS/Mt	386:390	CS/Mt	386:390	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	0	21	theme	chitosan-based	6:19	arg1	nanocomposites					21:34	Novel chitosan-based nanocomposites	0:34	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.	0:140	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	21	theme	chitosan-based	6:19	arg1	Synthesis					71:79	Synthesis	71:79	Synthesis	71:79	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	21	theme	chitosan-based	6:19	arg1	inhibition					91:100	root rot inhibition	82:100	root rot inhibition	82:100	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	21	theme	chitosan-based	6:19	arg1	management					113:122	growth management	106:122	growth management of tomato plants	106:139	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	8	22	theme	zero-order	667:676	arg1	equation					678:685	well zero-order equation	662:685	well zero-order equation	662:685	from CS and CS/Mt obeyed well zero-order equation.					
35123746	9	23	from	CS/PANI	767:773	arg1	data					757:760	the release data	745:760	the release data from CS/PANI and CS/Mt composites	745:794	However, Higuchi and Korsmeyer-Peppas models fitted well the release data from CS/PANI and CS/Mt composites.					
35123746	0	24	theme	Novel	0:4	arg1	nanocomposites					21:34	Novel chitosan-based nanocomposites	0:34	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.	0:140	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	24	theme	Novel	0:4	arg1	Synthesis					71:79	Synthesis	71:79	Synthesis	71:79	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	24	theme	Novel	0:4	arg1	inhibition					91:100	root rot inhibition	82:100	root rot inhibition	82:100	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	24	theme	Novel	0:4	arg1	management					113:122	growth management	106:122	growth management of tomato plants	106:139	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	11	25	theme	In	887:888	arg1	CS					903:904	CS	903:904	CS	903:904	In vitro assay, CS, Cinn., Van., CS/PANI and CS/PANI/Cinn.					
35123746	11	25	theme	In	887:888	arg1	assay					896:900	In vitro assay	887:900	In vitro assay	887:900	In vitro assay, CS, Cinn., Van., CS/PANI and CS/PANI/Cinn.					
35123746	0	26	theme	tomato	127:132	arg1	plants					134:139	tomato plants	127:139	tomato plants	127:139	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	12	27	theme	linear	986:991	arg1	growth					993:998	the linear growth	982:998	the linear growth of the target pathogens	982:1022	, have a strong inhibitory effect on the linear growth of the target pathogens, even at lower concentrations.					
35123746	0	28	theme	pesticide	51:59	arg1	carriers					61:68	ecofriendly pesticide carriers	39:68	ecofriendly pesticide carriers	39:68	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	13	29	dep	CS/PANI/Cinn	1120:1131	arg1	the					1109:1111	the	1109:1111	the	1109:1111	Greenhouse assay indicated that seedling treatment by the loaded CS/PANI/Cinn and CS/Mt/Cinn.					
35123746	4	30	with	montmorillonite	369:383	arg1	CS/PANI					438:444	CS/PANI	438:444	CS/PANI	438:444	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	4	30	with	montmorillonite	369:383	arg1	polyaniline					425:435	polyaniline	425:435	polyaniline (CS/PANI)	425:445	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	0	31	theme	ecofriendly	39:49	arg1	carriers					61:68	ecofriendly pesticide carriers	39:68	ecofriendly pesticide carriers	39:68	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	14	32	theme	incidence	1188:1196	arg1	parameters					1198:1207	disease incidence parameters	1180:1207	disease incidence parameters	1180:1207	reduced both disease index and disease incidence parameters of both pathogens and possessed seedlings growth promoting potential of tomato compared to untreated-infected controls.					
35123746	1	33	dep	developed	178:186	arg1	Van					247:249	Van	247:249	Van	247:249	Novel bio-based nanocomposites were developed as carriers for loading and sustained-release of vanillin (Van.)					
35123746	4	34	theme	chitosan	343:350	arg1	intercalation					326:338	intercalation	326:338	intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt)	326:510	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	10	35	theme	Pythium	867:873	arg1	debaryanum					875:884	Pythium debaryanum	867:884	Pythium debaryanum	867:884	Their antifungal activity was examined towards Fusarium oxysporum and Pythium debaryanum.					
35123746	0	36	theme	plants	134:139	arg1	nanocomposites					21:34	Novel chitosan-based nanocomposites	0:34	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.	0:140	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	36	theme	plants	134:139	arg1	Synthesis					71:79	Synthesis	71:79	Synthesis	71:79	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	36	theme	plants	134:139	arg1	inhibition					91:100	root rot inhibition	82:100	root rot inhibition	82:100	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	36	theme	plants	134:139	arg1	management					113:122	growth management	106:122	growth management of tomato plants	106:139	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	9	37	from	composites	785:794	arg1	data					757:760	the release data	745:760	the release data from CS/PANI and CS/Mt composites	745:794	However, Higuchi and Korsmeyer-Peppas models fitted well the release data from CS/PANI and CS/Mt composites.					
35123746	12	38	theme	inhibitory	961:970	arg1	effect					972:977	a strong inhibitory effect	952:977	a strong inhibitory effect	952:977	, have a strong inhibitory effect on the linear growth of the target pathogens, even at lower concentrations.					
35123746	4	39	theme	chitosan	411:418	arg1	montmorillonite					369:383	sodium montmorillonite	362:383	sodium montmorillonite (CS/Mt)	362:391	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	4	39	theme	chitosan	411:418	arg1	CS/PANI/Mt					500:509	CS/PANI/Mt	500:509	CS/PANI/Mt	500:509	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	4	39	theme	chitosan	411:418	arg1	montmorillonite					483:497	chitosan/polyaniline/exfoliated montmorillonite	451:497	chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt)	451:510	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	4	39	theme	chitosan	411:418	arg1	CS/Mt					386:390	CS/Mt	386:390	CS/Mt	386:390	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	4	39	theme	chitosan	411:418	arg1	incorporation					394:406	incorporation	394:406	incorporation of chitosan with polyaniline (CS/PANI)	394:445	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	14	40	theme	seedlings	1241:1249	arg1	growth					1251:1256	seedlings growth	1241:1256	seedlings growth promoting potential of tomato compared to untreated-infected controls	1241:1326	reduced both disease index and disease incidence parameters of both pathogens and possessed seedlings growth promoting potential of tomato compared to untreated-infected controls.					
35123746	12	41	theme	strong	954:959	arg1	effect					972:977	a strong inhibitory effect	952:977	a strong inhibitory effect	952:977	, have a strong inhibitory effect on the linear growth of the target pathogens, even at lower concentrations.					
35123746	9	42	dep	Higuchi	697:703	arg1	models					726:731	models	726:731	models	726:731	However, Higuchi and Korsmeyer-Peppas models fitted well the release data from CS/PANI and CS/Mt composites.					
35123746	13	43	theme	Greenhouse	1055:1064	arg1	assay					1066:1070	Greenhouse assay	1055:1070	Greenhouse assay	1055:1070	Greenhouse assay indicated that seedling treatment by the loaded CS/PANI/Cinn and CS/Mt/Cinn.					
35123746	1	44	theme	vanillin	237:244	arg1	sustained-release					216:232	sustained-release	216:232	sustained-release	216:232	Novel bio-based nanocomposites were developed as carriers for loading and sustained-release of vanillin (Van.)					
35123746	1	44	theme	vanillin	237:244	arg1	loading					204:210	loading	204:210	loading	204:210	Novel bio-based nanocomposites were developed as carriers for loading and sustained-release of vanillin (Van.)					
35123746	11	45	dep	Van.	914:917	arg1	CS					903:904	CS	903:904	CS	903:904	In vitro assay, CS, Cinn., Van., CS/PANI and CS/PANI/Cinn.					
35123746	11	45	dep	Van.	914:917	arg1	assay					896:900	In vitro assay	887:900	In vitro assay	887:900	In vitro assay, CS, Cinn., Van., CS/PANI and CS/PANI/Cinn.					
35123746	0	46	dep	nanocomposites	21:34	arg1	nanocomposites					21:34	Novel chitosan-based nanocomposites	0:34	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.	0:140	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	46	dep	nanocomposites	21:34	arg1	Synthesis					71:79	Synthesis	71:79	Synthesis	71:79	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	46	dep	nanocomposites	21:34	arg1	inhibition					91:100	root rot inhibition	82:100	root rot inhibition	82:100	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	0	46	dep	nanocomposites	21:34	arg1	management					113:122	growth management	106:122	growth management of tomato plants	106:139	Novel chitosan-based nanocomposites as ecofriendly pesticide carriers: Synthesis, root rot inhibition and growth management of tomato plants.					
35123746	1	47	theme	bio-based	148:156	arg1	carriers					191:198	carriers	191:198	carriers for loading and sustained-release of vanillin	191:244	Novel bio-based nanocomposites were developed as carriers for loading and sustained-release of vanillin (Van.)					
35123746	1	47	theme	bio-based	148:156	arg1	nanocomposites					158:171	Novel bio-based nanocomposites	142:171	Novel bio-based nanocomposites	142:171	Novel bio-based nanocomposites were developed as carriers for loading and sustained-release of vanillin (Van.)					
35123746	1	48	theme	Novel	142:146	arg1	carriers					191:198	carriers	191:198	carriers for loading and sustained-release of vanillin	191:244	Novel bio-based nanocomposites were developed as carriers for loading and sustained-release of vanillin (Van.)					
35123746	1	48	theme	Novel	142:146	arg1	nanocomposites					158:171	Novel bio-based nanocomposites	142:171	Novel bio-based nanocomposites	142:171	Novel bio-based nanocomposites were developed as carriers for loading and sustained-release of vanillin (Van.)					
35123746	9	49	theme	CS/Mt	779:783	arg1	composites					785:794	CS/Mt composites	779:794	CS/Mt composites	779:794	However, Higuchi and Korsmeyer-Peppas models fitted well the release data from CS/PANI and CS/Mt composites.					
35123746	4	50	with	incorporation	394:406	arg1	CS/PANI					438:444	CS/PANI	438:444	CS/PANI	438:444	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	4	50	with	incorporation	394:406	arg1	polyaniline					425:435	polyaniline	425:435	polyaniline (CS/PANI)	425:445	The composites were obtained by intercalation of chitosan (CS) into sodium montmorillonite (CS/Mt), incorporation of chitosan with polyaniline (CS/PANI) and chitosan/polyaniline/exfoliated montmorillonite (CS/PANI/Mt).					
35123746	6	51	theme	Van	622:624	arg1	data					614:617	data	614:617	data of Van	614:624	The release data of Van.					
37263129	7	0	theme	lactose	1229:1235	arg1	production					1138:1147	milk production	1133:1147	milk production	1133:1147	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	7	0	theme	lactose	1229:1235	arg1	performance					1162:1172	kits growth performance	1150:1172	kits growth performance	1150:1172	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	7	0	theme	lactose	1229:1235	arg1	content					1183:1189	the content	1179:1189	the content of total protein, solids-not-fat, and lactose in milk	1179:1243	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	8	1	theme	pregnancies	1330:1340	arg1	impact					1293:1298	the negative impact	1280:1298	the negative impact of overlapping lactations and pregnancies on rabbit kits	1280:1355	Therefore, we can speculate about the negative impact of overlapping lactations and pregnancies on rabbit kits, as their growth is dependent on milk production and composition.					
37263129	3	2	theme	consecutive	439:449	arg1	lactations					451:460	three consecutive lactations	433:460	three consecutive lactations of Hycole does	433:475	The study was conducted on a commercial rabbit farm and included three consecutive lactations of Hycole does.					
37263129	4	3	theme	milk	514:517	arg1	production					519:528	the daily milk production	504:528	the daily milk production	504:528	It has been observed that the daily milk production increased from the 2nd till the 14th day of lactation when does produced almost 300 g of milk daily.					
37263129	8	4	theme	negative	1284:1291	arg1	impact					1293:1298	the negative impact	1280:1298	the negative impact of overlapping lactations and pregnancies on rabbit kits	1280:1355	Therefore, we can speculate about the negative impact of overlapping lactations and pregnancies on rabbit kits, as their growth is dependent on milk production and composition.					
37263129	7	5	from	production	1138:1147	arg1	milk					1240:1243	milk	1240:1243	milk	1240:1243	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	2	6	theme	status	305:310	arg1	effect					278:283	the effect	274:283	the effect of the reproductive status of the females on daily milk yield and milk attributes	274:365	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	4	7	theme	daily	508:512	arg1	production					519:528	the daily milk production	504:528	the daily milk production	504:528	It has been observed that the daily milk production increased from the 2nd till the 14th day of lactation when does produced almost 300 g of milk daily.					
37263129	2	8	from	effect	278:283	arg1	yield					341:345	daily milk yield	330:345	daily milk yield	330:345	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	2	8	from	effect	278:283	arg1	attributes					356:365	milk attributes	351:365	milk attributes	351:365	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	2	9	theme	rabbit	250:255	arg1	milk					257:260	rabbit milk	250:260	rabbit milk	250:260	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	3	10	dep	Hycole	465:470	arg1	does					472:475	does	472:475	does	472:475	The study was conducted on a commercial rabbit farm and included three consecutive lactations of Hycole does.					
37263129	5	11	theme	lactation	642:650	arg1	day					635:637	The day	631:637	The day of lactation	631:650	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	6	12	theme	small	983:987	arg1	globules					998:1005	small milk fat globules	983:1005	small milk fat globules	983:1005	The percentage of small milk fat globules decreased with lactation day, causing a possible decrease in the digestions rates of milk.					
37263129	6	13	theme	milk	1092:1095	arg1	rates					1083:1087	the digestions rates	1068:1087	the digestions rates of milk	1068:1095	The percentage of small milk fat globules decreased with lactation day, causing a possible decrease in the digestions rates of milk.					
37263129	4	14	theme	milk	619:622	arg1	300 g					610:614	300 g	610:614	300 g	610:614	It has been observed that the daily milk production increased from the 2nd till the 14th day of lactation when does produced almost 300 g of milk daily.					
37263129	6	15	theme	fat	994:996	arg1	globules					998:1005	small milk fat globules	983:1005	small milk fat globules	983:1005	The percentage of small milk fat globules decreased with lactation day, causing a possible decrease in the digestions rates of milk.					
37263129	7	16	theme	solids-not-fat	1209:1222	arg1	production					1138:1147	milk production	1133:1147	milk production	1133:1147	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	7	16	theme	solids-not-fat	1209:1222	arg1	performance					1162:1172	kits growth performance	1150:1172	kits growth performance	1150:1172	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	7	16	theme	solids-not-fat	1209:1222	arg1	content					1183:1189	the content	1179:1189	the content of total protein, solids-not-fat, and lactose in milk	1179:1243	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	2	17	theme	reproductive	292:303	arg1	status					305:310	the reproductive status	288:310	the reproductive status of the females	288:325	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	6	18	theme	globules	998:1005	arg1	percentage					969:978	The percentage	965:978	The percentage of small milk fat globules	965:1005	The percentage of small milk fat globules decreased with lactation day, causing a possible decrease in the digestions rates of milk.					
37263129	7	19	theme	milk	1133:1136	arg1	production					1138:1147	milk production	1133:1147	milk production	1133:1147	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	7	20	contain	had	1108:1110	arg1	Pregnancy					1098:1106	Pregnancy	1098:1106	Pregnancy	1098:1106	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	7	20	contain	had	1108:1110	arg2	impact					1123:1128	a negative impact	1112:1128	a negative impact	1112:1128	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	4	21	theme	14th	562:565	arg1	day					567:569	the 2nd till the 14th day	545:569	the 2nd till the 14th day of lactation	545:582	It has been observed that the daily milk production increased from the 2nd till the 14th day of lactation when does produced almost 300 g of milk daily.					
37263129	1	22	theme	rabbit	69:74	arg1	milk					76:79	rabbit milk	69:79	rabbit milk	69:79	Increasing the knowledge of rabbit milk can help in breeding practice to solve issues considering the health and growth of rabbit kits.					
37263129	2	23	theme	milk	336:339	arg1	yield					341:345	daily milk yield	330:345	daily milk yield	330:345	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	6	24	theme	milk	989:992	arg1	globules					998:1005	small milk fat globules	983:1005	small milk fat globules	983:1005	The percentage of small milk fat globules decreased with lactation day, causing a possible decrease in the digestions rates of milk.					
37263129	6	25	theme	digestions	1072:1081	arg1	rates					1083:1087	the digestions rates	1068:1087	the digestions rates of milk	1068:1095	The percentage of small milk fat globules decreased with lactation day, causing a possible decrease in the digestions rates of milk.					
37263129	1	26	theme	milk	76:79	arg1	knowledge					56:64	the knowledge	52:64	Increasing the knowledge of rabbit milk	41:79	Increasing the knowledge of rabbit milk can help in breeding practice to solve issues considering the health and growth of rabbit kits.					
37263129	8	27	theme	lactations	1315:1324	arg1	impact					1293:1298	the negative impact	1280:1298	the negative impact of overlapping lactations and pregnancies on rabbit kits	1280:1355	Therefore, we can speculate about the negative impact of overlapping lactations and pregnancies on rabbit kits, as their growth is dependent on milk production and composition.					
37263129	2	28	theme	daily	330:334	arg1	yield					341:345	daily milk yield	330:345	daily milk yield	330:345	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	8	29	theme	overlapping	1303:1313	arg1	lactations					1315:1324	overlapping lactations	1303:1324	overlapping lactations	1303:1324	Therefore, we can speculate about the negative impact of overlapping lactations and pregnancies on rabbit kits, as their growth is dependent on milk production and composition.					
37263129	5	30	theme	fat	825:827	arg1	globules					829:836	fat globules	825:836	fat globules	825:836	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	1	31	dep	health	143:148	arg1	the					139:141	the	139:141	the	139:141	Increasing the knowledge of rabbit milk can help in breeding practice to solve issues considering the health and growth of rabbit kits.					
37263129	1	32	theme	rabbit	164:169	arg1	kits					171:174	rabbit kits	164:174	rabbit kits	164:174	Increasing the knowledge of rabbit milk can help in breeding practice to solve issues considering the health and growth of rabbit kits.					
37263129	8	33	from	impact	1293:1298	arg1	kits					1352:1355	rabbit kits	1345:1355	rabbit kits	1345:1355	Therefore, we can speculate about the negative impact of overlapping lactations and pregnancies on rabbit kits, as their growth is dependent on milk production and composition.					
37263129	8	34	theme	rabbit	1345:1350	arg1	kits					1352:1355	rabbit kits	1345:1355	rabbit kits	1345:1355	Therefore, we can speculate about the negative impact of overlapping lactations and pregnancies on rabbit kits, as their growth is dependent on milk production and composition.					
37263129	7	35	from	performance	1162:1172	arg1	milk					1240:1243	milk	1240:1243	milk	1240:1243	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	7	36	theme	protein	1200:1206	arg1	production					1138:1147	milk production	1133:1147	milk production	1133:1147	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	7	36	theme	protein	1200:1206	arg1	performance					1162:1172	kits growth performance	1150:1172	kits growth performance	1150:1172	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	7	36	theme	protein	1200:1206	arg1	content					1183:1189	the content	1179:1189	the content of total protein, solids-not-fat, and lactose in milk	1179:1243	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	7	37	from	content	1183:1189	arg1	milk					1240:1243	milk	1240:1243	milk	1240:1243	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	1	38	theme	kits	171:174	arg1	growth					154:159	growth	154:159	growth	154:159	Increasing the knowledge of rabbit milk can help in breeding practice to solve issues considering the health and growth of rabbit kits.					
37263129	1	38	theme	kits	171:174	arg1	health					143:148	health	143:148	health	143:148	Increasing the knowledge of rabbit milk can help in breeding practice to solve issues considering the health and growth of rabbit kits.					
37263129	5	39	theme	total	731:735	arg1	protein					737:743	total protein	731:743	total protein	731:743	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	2	40	theme	milk	257:260	arg1	analysis					238:245	a broad physicochemical analysis	214:245	a broad physicochemical analysis of rabbit milk	214:260	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	3	41	theme	rabbit	408:413	arg1	farm					415:418	a commercial rabbit farm	395:418	a commercial rabbit farm	395:418	The study was conducted on a commercial rabbit farm and included three consecutive lactations of Hycole does.					
37263129	0	42	theme	milk	35:38	arg1	attributes					14:23	the attributes	10:23	the attributes of rabbit milk	10:38	Unveiling the attributes of rabbit milk.					
37263129	5	43	dep	7 µm	959:962	arg1	to					956:957	to	956:957	to	956:957	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	6	44	from	decrease	1056:1063	arg1	rates					1083:1087	the digestions rates	1068:1087	the digestions rates of milk	1068:1095	The percentage of small milk fat globules decreased with lactation day, causing a possible decrease in the digestions rates of milk.					
37263129	2	45	theme	milk	351:354	arg1	attributes					356:365	milk attributes	351:365	milk attributes	351:365	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	0	46	theme	rabbit	28:33	arg1	milk					35:38	rabbit milk	28:38	rabbit milk	28:38	Unveiling the attributes of rabbit milk.					
37263129	2	47	theme	study	193:197	arg1	goal					181:184	The goal	177:184	The goal of the study	177:197	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	7	48	theme	negative	1114:1121	arg1	impact					1123:1128	a negative impact	1112:1128	a negative impact	1112:1128	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	5	49	theme	total	701:705	arg1	solids					707:712	total solids	701:712	total solids	701:712	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	6	50	theme	possible	1047:1054	arg1	decrease					1056:1063	a possible decrease	1045:1063	a possible decrease in the digestions rates of milk	1045:1095	The percentage of small milk fat globules decreased with lactation day, causing a possible decrease in the digestions rates of milk.					
37263129	1	51	theme	breeding	93:100	arg1	practice					102:109	breeding practice	93:109	breeding practice	93:109	Increasing the knowledge of rabbit milk can help in breeding practice to solve issues considering the health and growth of rabbit kits.					
37263129	5	52	theme	solids	707:712	arg1	content					690:696	the content	686:696	the content of total solids	686:712	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	5	53	theme	ongoing	895:901	arg1	lactation					903:911	the ongoing lactation	891:911	the ongoing lactation	891:911	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	7	54	theme	kits	1150:1153	arg1	performance					1162:1172	kits growth performance	1150:1172	kits growth performance	1150:1172	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	6	55	theme	lactation	1022:1030	arg1	day					1032:1034	lactation day	1022:1034	lactation day	1022:1034	The percentage of small milk fat globules decreased with lactation day, causing a possible decrease in the digestions rates of milk.					
37263129	5	56	theme	globules	829:836	arg1	protein					737:743	total protein	731:743	total protein	731:743	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	5	56	theme	globules	829:836	arg1	casein					746:751	casein	746:751	casein	746:751	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	5	56	theme	globules	829:836	arg1	percentage					811:820	The percentage	807:820	The percentage of fat globules	807:836	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	5	56	theme	globules	829:836	arg1	C18					771:773	C18	771:773	C18	771:773	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	5	56	theme	globules	829:836	arg1	solids-not-fat					715:728	solids-not-fat	715:728	solids-not-fat	715:728	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	5	56	theme	globules	829:836	arg1	Count					792:796	Somatic Cell Count	779:796	Somatic Cell Count	779:796	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	5	56	theme	globules	829:836	arg1	lactose					754:760	lactose	754:760	lactose	754:760	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	5	56	theme	globules	829:836	arg1	C18					763:765	C18	763:765	C18	763:765	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	2	57	theme	broad	216:220	arg1	analysis					238:245	a broad physicochemical analysis	214:245	a broad physicochemical analysis of rabbit milk	214:260	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	7	58	theme	total	1194:1198	arg1	protein					1200:1206	total protein	1194:1206	total protein	1194:1206	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	5	59	theme	Somatic	779:785	arg1	Count					792:796	Somatic Cell Count	779:796	Somatic Cell Count	779:796	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	8	60	theme	milk	1390:1393	arg1	production					1395:1404	milk production	1390:1404	milk production	1390:1404	Therefore, we can speculate about the negative impact of overlapping lactations and pregnancies on rabbit kits, as their growth is dependent on milk production and composition.					
37263129	2	61	theme	physicochemical	222:236	arg1	analysis					238:245	a broad physicochemical analysis	214:245	a broad physicochemical analysis of rabbit milk	214:260	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	5	62	theme	Cell	787:790	arg1	Count					792:796	Somatic Cell Count	779:796	Somatic Cell Count	779:796	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	5	63	theme	significant	661:671	arg1	variation					673:681	a significant variation	659:681	a significant variation in the content of total solids	659:712	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	5	64	from	variation	673:681	arg1	content					690:696	the content	686:696	the content of total solids	686:712	The day of lactation caused a significant variation in the content of total solids, solids-not-fat, total protein, casein, lactose, C18: 2, C18: 3, Somatic Cell Count, and pH. The percentage of fat globules categorised according to their diameter changed with the ongoing lactation as well, and the diameter increased from 5 to 7 µm.					
37263129	7	65	theme	growth	1155:1160	arg1	performance					1162:1172	kits growth performance	1150:1172	kits growth performance	1150:1172	Pregnancy had a negative impact on milk production, kits growth performance, and the content of total protein, solids-not-fat, and lactose in milk.					
37263129	3	66	theme	commercial	397:406	arg1	farm					415:418	a commercial rabbit farm	395:418	a commercial rabbit farm	395:418	The study was conducted on a commercial rabbit farm and included three consecutive lactations of Hycole does.					
37263129	2	67	theme	females	319:325	arg1	status					305:310	the reproductive status	288:310	the reproductive status of the females	288:325	The goal of the study was to perform a broad physicochemical analysis of rabbit milk and examine the effect of the reproductive status of the females on daily milk yield and milk attributes.					
37263129	4	68	theme	lactation	574:582	arg1	day					567:569	the 2nd till the 14th day	545:569	the 2nd till the 14th day of lactation	545:582	It has been observed that the daily milk production increased from the 2nd till the 14th day of lactation when does produced almost 300 g of milk daily.					
37263129	4	69	theme	till	553:556	arg1	day					567:569	the 2nd till the 14th day	545:569	the 2nd till the 14th day of lactation	545:582	It has been observed that the daily milk production increased from the 2nd till the 14th day of lactation when does produced almost 300 g of milk daily.					
37263129	1	70	theme	Increasing	41:50	arg1	knowledge					56:64	the knowledge	52:64	Increasing the knowledge of rabbit milk	41:79	Increasing the knowledge of rabbit milk can help in breeding practice to solve issues considering the health and growth of rabbit kits.					
37263129	3	71	theme	Hycole	465:470	arg1	lactations					451:460	three consecutive lactations	433:460	three consecutive lactations of Hycole does	433:475	The study was conducted on a commercial rabbit farm and included three consecutive lactations of Hycole does.					
37263129	4	72	theme	2nd	549:551	arg1	day					567:569	the 2nd till the 14th day	545:569	the 2nd till the 14th day of lactation	545:582	It has been observed that the daily milk production increased from the 2nd till the 14th day of lactation when does produced almost 300 g of milk daily.					
37086562	0	0	theme	strain	75:80	arg1	O-antigen					45:53	the O-antigen	41:53	the O-antigen of Aeromonas sobria strain K928	41:85	Structure and gene cluster annotation of the O-antigen of Aeromonas sobria strain K928 isolated from common carp and classified into the new Aeromonas PGO1 serogroup.					
37086562	4	1	from	sequences	965:973	arg1	databases					992:1000	the available databases	978:1000	the available databases	978:1000	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	3	2	theme	A.	722:723	arg1	K928					732:735	A. sobria K928	722:735	A. sobria K928	722:735	The following structure of the branched O-specific polysaccharide repeating unit of A. sobria K928 was established.					
37086562	2	3	theme	sugar	574:578	arg1	analyses					596:603	sugar and methylation analyses	574:603	analyses	596:603	The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy.					
37086562	0	4	theme	sobria	68:73	arg1	strain					75:80	Aeromonas sobria strain K928	58:85	Aeromonas sobria strain K928	58:85	Structure and gene cluster annotation of the O-antigen of Aeromonas sobria strain K928 isolated from common carp and classified into the new Aeromonas PGO1 serogroup.					
37086562	3	5	theme	O-specific	678:687	arg1	polysaccharide					689:702	the branched O-specific polysaccharide	665:702	the branched O-specific polysaccharide repeating unit of A. sobria K928	665:735	The following structure of the branched O-specific polysaccharide repeating unit of A. sobria K928 was established.					
37086562	5	6	theme	O-antigen	1026:1034	arg1	region					1044:1049	the O-antigen genetic region	1022:1049	the O-antigen genetic region	1022:1049	The composition of the O-antigen genetic region was found to be consistent with the O-polysaccharide structure, and its organization was proposed.					
37086562	5	7	theme	genetic	1036:1042	arg1	region					1044:1049	the O-antigen genetic region	1022:1049	the O-antigen genetic region	1022:1049	The composition of the O-antigen genetic region was found to be consistent with the O-polysaccharide structure, and its organization was proposed.					
37086562	3	8	theme	polysaccharide	689:702	arg1	structure					652:660	The following structure	638:660	The following structure of the branched O-specific polysaccharide repeating unit of A. sobria K928	638:735	The following structure of the branched O-specific polysaccharide repeating unit of A. sobria K928 was established.					
37086562	2	9	theme	methylation	584:594	arg1	analyses					596:603	sugar and methylation analyses	574:603	analyses	596:603	The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy.					
37086562	4	10	theme	[α-D-Fucp3NRHb-	757:771	arg1	-β-D-FucpNAc-					823:835	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→	754:838	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.	754:1001	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	1	11	theme	Motile	237:242	arg1	outbreak					301:308	a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak	235:308	a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak	235:308	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	3	12	theme	sobria	725:730	arg1	K928					732:735	A. sobria K928	722:735	A. sobria K928	722:735	The following structure of the branched O-specific polysaccharide repeating unit of A. sobria K928 was established.					
37086562	4	13	theme	O-antigen	844:852	arg1	cluster					859:865	The O-antigen gene cluster	840:865	The O-antigen gene cluster	840:865	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	1	14	theme	Aeromonas	244:252	arg1	outbreak					301:308	a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak	235:308	a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak	235:308	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	0	15	theme	common	101:106	arg1	carp					108:111	common carp	101:111	common carp	101:111	Structure and gene cluster annotation of the O-antigen of Aeromonas sobria strain K928 isolated from common carp and classified into the new Aeromonas PGO1 serogroup.					
37086562	3	16	theme	K928	732:735	arg1	unit					714:717	unit	714:717	unit of A. sobria K928	714:735	The following structure of the branched O-specific polysaccharide repeating unit of A. sobria K928 was established.					
37086562	2	17	theme	NMR	620:622	arg1	spectroscopy					624:635	NMR spectroscopy	620:635	NMR spectroscopy	620:635	The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy.					
37086562	4	18	theme	available	982:990	arg1	databases					992:1000	the available databases	978:1000	the available databases	978:1000	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	4	19	theme	1→3	773:775	arg1	-β-D-FucpNAc-					823:835	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→	754:838	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.	754:1001	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	1	20	theme	new	356:358	arg1	serogroup					377:385	the new provisional PGO1 serogroup	352:385	the new provisional PGO1 serogroup	352:385	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	4	21	with	comparison	949:958	arg1	sequences					965:973	sequences	965:973	sequences in the available databases	965:1000	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	5	22	theme	region	1044:1049	arg1	consistent					1067:1076	consistent	1067:1076	consistent	1067:1076	The composition of the O-antigen genetic region was found to be consistent with the O-polysaccharide structure, and its organization was proposed.					
37086562	5	22	theme	region	1044:1049	arg1	composition					1007:1017	The composition	1003:1017	The composition of the O-antigen genetic region	1003:1049	The composition of the O-antigen genetic region was found to be consistent with the O-polysaccharide structure, and its organization was proposed.					
37086562	1	23	theme	provisional	360:370	arg1	serogroup					377:385	the new provisional PGO1 serogroup	352:385	the new provisional PGO1 serogroup	352:385	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	4	24	dep	A.	921:922	arg1	sobria					924:929	sobria	924:929	sobria	924:929	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	1	25	theme	Aeromonas	167:175	arg1	K928					191:194	Aeromonas sobria strain K928	167:194	Aeromonas sobria strain K928	167:194	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	2	26	dep	1H	609:610	arg1	spectroscopy					624:635	NMR spectroscopy	620:635	NMR spectroscopy	620:635	The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy.					
37086562	1	27	theme	Infection/Motile	254:269	arg1	outbreak					301:308	a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak	235:308	a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak	235:308	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	4	28	theme	-α-L-Rhap-	808:817	arg1	-β-D-FucpNAc-					823:835	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→	754:838	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.	754:1001	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	1	29	theme	sobria	177:182	arg1	K928					191:194	Aeromonas sobria strain K928	167:194	Aeromonas sobria strain K928	167:194	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	0	30	theme	cluster	19:25	arg1	annotation					27:36	gene cluster annotation	14:36	gene cluster annotation	14:36	Structure and gene cluster annotation of the O-antigen of Aeromonas sobria strain K928 isolated from common carp and classified into the new Aeromonas PGO1 serogroup.					
37086562	1	31	theme	Aeromonas	271:279	arg1	outbreak					301:308	a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak	235:308	a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak	235:308	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	4	32	theme	→2	754:755	arg1	-β-D-FucpNAc-					823:835	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→	754:838	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.	754:1001	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	1	33	theme	strain	184:189	arg1	K928					191:194	Aeromonas sobria strain K928	167:194	Aeromonas sobria strain K928	167:194	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	0	34	theme	gene	14:17	arg1	annotation					27:36	gene cluster annotation	14:36	gene cluster annotation	14:36	Structure and gene cluster annotation of the O-antigen of Aeromonas sobria strain K928 isolated from common carp and classified into the new Aeromonas PGO1 serogroup.					
37086562	1	35	theme	Septicaemia	281:291	arg1	outbreak					301:308	a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak	235:308	a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak	235:308	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	2	36	theme	A.	414:415	arg1	K928					424:427	A. sobria K928	414:427	A. sobria K928	414:427	The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy.					
37086562	4	37	theme	strain	936:941	arg1	genome					907:912	the genome	903:912	the genome of the A. sobria K928 strain	903:941	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	1	38	theme	disease	293:299	arg1	outbreak					301:308	a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak	235:308	a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak	235:308	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	2	39	theme	gel-permeation	525:538	arg1	chromatography					540:553	gel-permeation chromatography	525:553	gel-permeation chromatography	525:553	The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy.					
37086562	3	40	theme	following	642:650	arg1	structure					652:660	The following structure	638:660	The following structure of the branched O-specific polysaccharide repeating unit of A. sobria K928	638:735	The following structure of the branched O-specific polysaccharide repeating unit of A. sobria K928 was established.					
37086562	4	41	theme	-α-L-Rhap-	778:787	arg1	-β-D-FucpNAc-					823:835	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→	754:838	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.	754:1001	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	4	42	theme	K928	931:934	arg1	strain					936:941	the A. sobria K928 strain	917:941	the A. sobria K928 strain	917:941	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	4	43	theme	A.	921:922	arg1	strain					936:941	the A. sobria K928 strain	917:941	the A. sobria K928 strain	917:941	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	4	44	dep	-β-D-FucpNAc-	823:835	arg1	characterized					886:898	characterized	886:898	characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases	886:1000	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	4	44	dep	-β-D-FucpNAc-	823:835	arg1	identified					871:880	identified	871:880	identified	871:880	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	4	45	theme	1→3	789:791	arg1	-β-D-FucpNAc-					823:835	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→	754:838	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.	754:1001	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	0	46	theme	O-antigen	45:53	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and gene cluster annotation of the O-antigen of Aeromonas sobria strain K928 isolated from common carp and classified into the new Aeromonas PGO1 serogroup.					
37086562	0	46	theme	O-antigen	45:53	arg1	annotation					27:36	gene cluster annotation	14:36	gene cluster annotation	14:36	Structure and gene cluster annotation of the O-antigen of Aeromonas sobria strain K928 isolated from common carp and classified into the new Aeromonas PGO1 serogroup.					
37086562	5	47	theme	O-polysaccharide	1087:1102	arg1	structure					1104:1112	the O-polysaccharide structure	1083:1112	the O-polysaccharide structure	1083:1112	The composition of the O-antigen genetic region was found to be consistent with the O-polysaccharide structure, and its organization was proposed.					
37086562	0	48	theme	new	137:139	arg1	serogroup					156:164	the new Aeromonas PGO1 serogroup	133:164	the new Aeromonas PGO1 serogroup	133:164	Structure and gene cluster annotation of the O-antigen of Aeromonas sobria strain K928 isolated from common carp and classified into the new Aeromonas PGO1 serogroup.					
37086562	2	49	theme	O-specific	476:485	arg1	polysaccharide					487:500	the O-specific polysaccharide	472:500	the O-specific polysaccharide	472:500	The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy.					
37086562	2	50	theme	mild	446:449	arg1	hydrolysis					456:465	mild acid hydrolysis	446:465	mild acid hydrolysis	446:465	The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy.					
37086562	1	51	from	carp	223:226	arg1	farm					327:330	a Polish fish farm	313:330	a Polish fish farm	313:330	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	4	52	theme	-β-L-Rhap-	793:802	arg1	-β-D-FucpNAc-					823:835	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→	754:838	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.	754:1001	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	1	53	theme	Polish	315:320	arg1	farm					327:330	a Polish fish farm	313:330	a Polish fish farm	313:330	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	4	54	theme	gene	854:857	arg1	cluster					859:865	The O-antigen gene cluster	840:865	The O-antigen gene cluster	840:865	→2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases.					
37086562	3	55	theme	branched	669:676	arg1	polysaccharide					689:702	the branched O-specific polysaccharide	665:702	the branched O-specific polysaccharide repeating unit of A. sobria K928	665:735	The following structure of the branched O-specific polysaccharide repeating unit of A. sobria K928 was established.					
37086562	0	56	theme	Aeromonas	141:149	arg1	serogroup					156:164	the new Aeromonas PGO1 serogroup	133:164	the new Aeromonas PGO1 serogroup	133:164	Structure and gene cluster annotation of the O-antigen of Aeromonas sobria strain K928 isolated from common carp and classified into the new Aeromonas PGO1 serogroup.					
37086562	1	57	attach	isolated	200:207	arg2	K928					191:194	Aeromonas sobria strain K928	167:194	Aeromonas sobria strain K928	167:194	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	1	57	attach	isolated	200:207	arg1	carp					223:226	a common carp	214:226	a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm	214:330	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	0	58	theme	Aeromonas	58:66	arg1	strain					75:80	Aeromonas sobria strain K928	58:85	Aeromonas sobria strain K928	58:85	Structure and gene cluster annotation of the O-antigen of Aeromonas sobria strain K928 isolated from common carp and classified into the new Aeromonas PGO1 serogroup.					
37086562	2	59	theme	sobria	417:422	arg1	K928					424:427	A. sobria K928	414:427	A. sobria K928	414:427	The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy.					
37086562	1	60	theme	common	216:221	arg1	carp					223:226	a common carp	214:226	a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm	214:330	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	2	61	theme	acid	451:454	arg1	hydrolysis					456:465	mild acid hydrolysis	446:465	mild acid hydrolysis	446:465	The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy.					
37086562	1	62	theme	fish	322:325	arg1	farm					327:330	a Polish fish farm	313:330	a Polish fish farm	313:330	Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup.					
37086562	2	63	theme	K928	424:427	arg1	lipopolysaccharide					392:409	The lipopolysaccharide	388:409	The lipopolysaccharide of A. sobria K928	388:427	The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy.					
37086562	5	64	with	consistent	1067:1076	arg1	structure					1104:1112	the O-polysaccharide structure	1083:1112	the O-polysaccharide structure	1083:1112	The composition of the O-antigen genetic region was found to be consistent with the O-polysaccharide structure, and its organization was proposed.					
36871174	8	0	theme	repair	1109:1114	arg1	similar					1162:1168	similar	1162:1168	similar	1162:1168	Morphological and functional examination showed that the repair effects of the TEN grafts and autografts were similar.					
36871174	8	0	theme	repair	1109:1114	arg1	effects					1116:1122	the repair effects	1105:1122	the repair effects of the TEN grafts and autografts	1105:1155	Morphological and functional examination showed that the repair effects of the TEN grafts and autografts were similar.					
36871174	6	1	theme	cell-derived	854:865	arg1	matrix					881:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	The lumen of the structure was filled with human bone mesenchymal stem cell-derived extracellular matrix and fibers to provide nutrition and topographic guidance, respectively.					
36871174	5	2	theme	scaffold	773:780	arg1	layer					753:757	the outer layer	743:757	the outer layer of the chitin scaffold	743:780	For this purpose, a chitin helical scaffold was fabricated by means of mold casting and acetylation using chitosan as the raw material, following which a fibrous membrane was electrospun on the outer layer of the chitin scaffold.					
36871174	1	3	theme	autologous	149:158	arg1	treatment					213:221	the gold-standard treatment	195:221	the gold-standard treatment for them	195:230	Peripheral nerve defects are a worldwide problem, and autologous nerve transplantation is currently the gold-standard treatment for them.					
36871174	1	3	theme	autologous	149:158	arg1	transplantation					166:180	autologous nerve transplantation	149:180	autologous nerve transplantation	149:180	Peripheral nerve defects are a worldwide problem, and autologous nerve transplantation is currently the gold-standard treatment for them.					
36871174	0	4	theme	Tissue	75:80	arg1	Engineering					82:92	Novel Bionic Tissue Engineering	62:92	Novel Bionic Tissue Engineering	62:92	Experimental Study on Repairing Peripheral Nerve Defects with Novel Bionic Tissue Engineering.					
36871174	0	5	from	Study	13:17	arg1	Nerve					43:47	Repairing Peripheral Nerve	22:47	Repairing Peripheral Nerve	22:47	Experimental Study on Repairing Peripheral Nerve Defects with Novel Bionic Tissue Engineering.					
36871174	1	6	theme	nerve	160:164	arg1	treatment					213:221	the gold-standard treatment	195:221	the gold-standard treatment for them	195:230	Peripheral nerve defects are a worldwide problem, and autologous nerve transplantation is currently the gold-standard treatment for them.					
36871174	1	6	theme	nerve	160:164	arg1	transplantation					166:180	autologous nerve transplantation	149:180	autologous nerve transplantation	149:180	Peripheral nerve defects are a worldwide problem, and autologous nerve transplantation is currently the gold-standard treatment for them.					
36871174	4	7	theme	bionic	478:483	arg1	graft					489:493	a novel bionic TEN graft	470:493	a novel bionic TEN graft with a biomimetic structure and composition	470:537	In this study, a novel bionic TEN graft with a biomimetic structure and composition was designed.					
36871174	6	8	theme	bone	832:835	arg1	matrix					881:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	The lumen of the structure was filled with human bone mesenchymal stem cell-derived extracellular matrix and fibers to provide nutrition and topographic guidance, respectively.					
36871174	5	9	theme	mold	624:627	arg1	casting					629:635	mold casting	624:635	mold casting	624:635	For this purpose, a chitin helical scaffold was fabricated by means of mold casting and acetylation using chitosan as the raw material, following which a fibrous membrane was electrospun on the outer layer of the chitin scaffold.					
36871174	2	10	theme	promising	292:300	arg1	methods					302:308	promising methods	292:308	promising methods for the same	292:321	Tissue-engineered nerve (TEN) grafts are widely considered promising methods for the same, and have attracted much attention.					
36871174	6	11	theme	human	826:830	arg1	matrix					881:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	The lumen of the structure was filled with human bone mesenchymal stem cell-derived extracellular matrix and fibers to provide nutrition and topographic guidance, respectively.					
36871174	5	12	theme	helical	580:586	arg1	scaffold					588:595	a chitin helical scaffold	571:595	a chitin helical scaffold	571:595	For this purpose, a chitin helical scaffold was fabricated by means of mold casting and acetylation using chitosan as the raw material, following which a fibrous membrane was electrospun on the outer layer of the chitin scaffold.					
36871174	5	13	theme	chitin	573:578	arg1	scaffold					588:595	a chitin helical scaffold	571:595	a chitin helical scaffold	571:595	For this purpose, a chitin helical scaffold was fabricated by means of mold casting and acetylation using chitosan as the raw material, following which a fibrous membrane was electrospun on the outer layer of the chitin scaffold.					
36871174	5	14	theme	outer	747:751	arg1	layer					753:757	the outer layer	743:757	the outer layer of the chitin scaffold	743:780	For this purpose, a chitin helical scaffold was fabricated by means of mold casting and acetylation using chitosan as the raw material, following which a fibrous membrane was electrospun on the outer layer of the chitin scaffold.					
36871174	9	15	theme	new	1267:1269	arg1	way					1271:1273	a new way	1265:1273	a new way to repair clinical peripheral nerve defects	1265:1317	The bionic TEN graft described in this study shows great potential for application and offers a new way to repair clinical peripheral nerve defects.					
36871174	8	16	theme	grafts	1135:1140	arg1	similar					1162:1168	similar	1162:1168	similar	1162:1168	Morphological and functional examination showed that the repair effects of the TEN grafts and autografts were similar.					
36871174	8	16	theme	grafts	1135:1140	arg1	effects					1116:1122	the repair effects	1105:1122	the repair effects of the TEN grafts and autografts	1105:1155	Morphological and functional examination showed that the repair effects of the TEN grafts and autografts were similar.					
36871174	7	17	from	defects	1035:1041	arg1	rats					1046:1049	rats	1046:1049	rats	1046:1049	The prepared TEN graft was then transplanted to bridge 10-mm sciatic nerve defects in rats.					
36871174	8	18	theme	Morphological	1052:1064	arg1	examination					1081:1091	Morphological and functional examination	1052:1091	Morphological and functional examination	1052:1091	Morphological and functional examination showed that the repair effects of the TEN grafts and autografts were similar.					
36871174	0	19	theme	Experimental	0:11	arg1	Study					13:17	Experimental Study	0:17	Experimental Study on Repairing Peripheral Nerve	0:47	Experimental Study on Repairing Peripheral Nerve Defects with Novel Bionic Tissue Engineering.					
36871174	6	20	theme	topographic	924:934	arg1	guidance					936:943	topographic guidance	924:943	topographic guidance	924:943	The lumen of the structure was filled with human bone mesenchymal stem cell-derived extracellular matrix and fibers to provide nutrition and topographic guidance, respectively.					
36871174	1	21	theme	Peripheral	95:104	arg1	problem					136:142	a worldwide problem	124:142	a worldwide problem	124:142	Peripheral nerve defects are a worldwide problem, and autologous nerve transplantation is currently the gold-standard treatment for them.					
36871174	1	21	theme	Peripheral	95:104	arg1	defects					112:118	Peripheral nerve defects	95:118	Peripheral nerve defects	95:118	Peripheral nerve defects are a worldwide problem, and autologous nerve transplantation is currently the gold-standard treatment for them.					
36871174	6	22	link	cell-derived	854:865	arg1	matrix					881:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	The lumen of the structure was filled with human bone mesenchymal stem cell-derived extracellular matrix and fibers to provide nutrition and topographic guidance, respectively.					
36871174	0	23	theme	Repairing	22:30	arg1	Nerve					43:47	Repairing Peripheral Nerve	22:47	Repairing Peripheral Nerve	22:47	Experimental Study on Repairing Peripheral Nerve Defects with Novel Bionic Tissue Engineering.					
36871174	9	24	theme	repair	1278:1283	arg1	defects					1311:1317	repair clinical peripheral nerve defects	1278:1317	repair clinical peripheral nerve defects	1278:1317	The bionic TEN graft described in this study shows great potential for application and offers a new way to repair clinical peripheral nerve defects.					
36871174	7	25	theme	sciatic	1021:1027	arg1	defects					1035:1041	bridge 10-mm sciatic nerve defects	1008:1041	bridge 10-mm sciatic nerve defects in rats	1008:1049	The prepared TEN graft was then transplanted to bridge 10-mm sciatic nerve defects in rats.					
36871174	9	26	theme	bionic	1175:1180	arg1	graft					1186:1190	The bionic TEN graft	1171:1190	The bionic TEN graft described in this study	1171:1214	The bionic TEN graft described in this study shows great potential for application and offers a new way to repair clinical peripheral nerve defects.					
36871174	1	27	theme	nerve	106:110	arg1	problem					136:142	a worldwide problem	124:142	a worldwide problem	124:142	Peripheral nerve defects are a worldwide problem, and autologous nerve transplantation is currently the gold-standard treatment for them.					
36871174	1	27	theme	nerve	106:110	arg1	defects					112:118	Peripheral nerve defects	95:118	Peripheral nerve defects	95:118	Peripheral nerve defects are a worldwide problem, and autologous nerve transplantation is currently the gold-standard treatment for them.					
36871174	1	28	theme	gold-standard	199:211	arg1	treatment					213:221	the gold-standard treatment	195:221	the gold-standard treatment for them	195:230	Peripheral nerve defects are a worldwide problem, and autologous nerve transplantation is currently the gold-standard treatment for them.					
36871174	1	28	theme	gold-standard	199:211	arg1	transplantation					166:180	autologous nerve transplantation	149:180	autologous nerve transplantation	149:180	Peripheral nerve defects are a worldwide problem, and autologous nerve transplantation is currently the gold-standard treatment for them.					
36871174	5	29	theme	chitin	766:771	arg1	scaffold					773:780	the chitin scaffold	762:780	the chitin scaffold	762:780	For this purpose, a chitin helical scaffold was fabricated by means of mold casting and acetylation using chitosan as the raw material, following which a fibrous membrane was electrospun on the outer layer of the chitin scaffold.					
36871174	9	30	theme	clinical	1285:1292	arg1	defects					1311:1317	repair clinical peripheral nerve defects	1278:1317	repair clinical peripheral nerve defects	1278:1317	The bionic TEN graft described in this study shows great potential for application and offers a new way to repair clinical peripheral nerve defects.					
36871174	7	31	theme	nerve	1029:1033	arg1	defects					1035:1041	bridge 10-mm sciatic nerve defects	1008:1041	bridge 10-mm sciatic nerve defects in rats	1008:1049	The prepared TEN graft was then transplanted to bridge 10-mm sciatic nerve defects in rats.					
36871174	4	32	with	graft	489:493	arg1	composition					527:537	composition	527:537	composition	527:537	In this study, a novel bionic TEN graft with a biomimetic structure and composition was designed.					
36871174	4	32	with	graft	489:493	arg1	structure					513:521	a biomimetic structure	500:521	a biomimetic structure	500:521	In this study, a novel bionic TEN graft with a biomimetic structure and composition was designed.					
36871174	9	33	theme	peripheral	1294:1303	arg1	defects					1311:1317	repair clinical peripheral nerve defects	1278:1317	repair clinical peripheral nerve defects	1278:1317	The bionic TEN graft described in this study shows great potential for application and offers a new way to repair clinical peripheral nerve defects.					
36871174	7	34	theme	bridge	1008:1013	arg1	defects					1035:1041	bridge 10-mm sciatic nerve defects	1008:1041	bridge 10-mm sciatic nerve defects in rats	1008:1049	The prepared TEN graft was then transplanted to bridge 10-mm sciatic nerve defects in rats.					
36871174	6	35	theme	structure	800:808	arg1	lumen					787:791	The lumen	783:791	The lumen of the structure	783:808	The lumen of the structure was filled with human bone mesenchymal stem cell-derived extracellular matrix and fibers to provide nutrition and topographic guidance, respectively.					
36871174	0	36	theme	Peripheral	32:41	arg1	Nerve					43:47	Repairing Peripheral Nerve	22:47	Repairing Peripheral Nerve	22:47	Experimental Study on Repairing Peripheral Nerve Defects with Novel Bionic Tissue Engineering.					
36871174	9	37	theme	nerve	1305:1309	arg1	defects					1311:1317	repair clinical peripheral nerve defects	1278:1317	repair clinical peripheral nerve defects	1278:1317	The bionic TEN graft described in this study shows great potential for application and offers a new way to repair clinical peripheral nerve defects.					
36871174	5	38	theme	raw	675:677	arg1	material					679:686	the raw material	671:686	the raw material	671:686	For this purpose, a chitin helical scaffold was fabricated by means of mold casting and acetylation using chitosan as the raw material, following which a fibrous membrane was electrospun on the outer layer of the chitin scaffold.					
36871174	5	38	theme	raw	675:677	arg1	chitosan					659:666	chitosan	659:666	chitosan	659:666	For this purpose, a chitin helical scaffold was fabricated by means of mold casting and acetylation using chitosan as the raw material, following which a fibrous membrane was electrospun on the outer layer of the chitin scaffold.					
36871174	2	39	theme	nerve	251:255	arg1	grafts					263:268	Tissue-engineered nerve (TEN) grafts	233:268	Tissue-engineered nerve (TEN) grafts	233:268	Tissue-engineered nerve (TEN) grafts are widely considered promising methods for the same, and have attracted much attention.					
36871174	5	40	theme	fibrous	707:713	arg1	membrane					715:722	a fibrous membrane	705:722	a fibrous membrane	705:722	For this purpose, a chitin helical scaffold was fabricated by means of mold casting and acetylation using chitosan as the raw material, following which a fibrous membrane was electrospun on the outer layer of the chitin scaffold.					
36871174	2	41	theme	Tissue-engineered	233:249	arg1	grafts					263:268	Tissue-engineered nerve (TEN) grafts	233:268	Tissue-engineered nerve (TEN) grafts	233:268	Tissue-engineered nerve (TEN) grafts are widely considered promising methods for the same, and have attracted much attention.					
36871174	2	42	theme	much	343:346	arg1	attention					348:356	much attention	343:356	much attention	343:356	Tissue-engineered nerve (TEN) grafts are widely considered promising methods for the same, and have attracted much attention.					
36871174	3	43	theme	bionics	399:405	arg1	incorporation					382:394	the incorporation	378:394	the incorporation of bionics into TEN grafts	378:421	To improve repair, the incorporation of bionics into TEN grafts has become a focus of research.					
36871174	4	44	theme	biomimetic	502:511	arg1	structure					513:521	a biomimetic structure	500:521	a biomimetic structure	500:521	In this study, a novel bionic TEN graft with a biomimetic structure and composition was designed.					
36871174	6	45	theme	extracellular	867:879	arg1	matrix					881:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	The lumen of the structure was filled with human bone mesenchymal stem cell-derived extracellular matrix and fibers to provide nutrition and topographic guidance, respectively.					
36871174	6	46	theme	stem	849:852	arg1	matrix					881:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	The lumen of the structure was filled with human bone mesenchymal stem cell-derived extracellular matrix and fibers to provide nutrition and topographic guidance, respectively.					
36871174	7	47	theme	prepared	964:971	arg1	graft					977:981	The prepared TEN graft	960:981	The prepared TEN graft	960:981	The prepared TEN graft was then transplanted to bridge 10-mm sciatic nerve defects in rats.					
36871174	1	48	theme	worldwide	126:134	arg1	problem					136:142	a worldwide problem	124:142	a worldwide problem	124:142	Peripheral nerve defects are a worldwide problem, and autologous nerve transplantation is currently the gold-standard treatment for them.					
36871174	1	48	theme	worldwide	126:134	arg1	defects					112:118	Peripheral nerve defects	95:118	Peripheral nerve defects	95:118	Peripheral nerve defects are a worldwide problem, and autologous nerve transplantation is currently the gold-standard treatment for them.					
36871174	8	49	theme	functional	1070:1079	arg1	examination					1081:1091	Morphological and functional examination	1052:1091	Morphological and functional examination	1052:1091	Morphological and functional examination showed that the repair effects of the TEN grafts and autografts were similar.					
36871174	0	50	theme	Bionic	68:73	arg1	Engineering					82:92	Novel Bionic Tissue Engineering	62:92	Novel Bionic Tissue Engineering	62:92	Experimental Study on Repairing Peripheral Nerve Defects with Novel Bionic Tissue Engineering.					
36871174	9	51	theme	great	1222:1226	arg1	potential					1228:1236	great potential	1222:1236	great potential for application	1222:1252	The bionic TEN graft described in this study shows great potential for application and offers a new way to repair clinical peripheral nerve defects.					
36871174	3	52	theme	research	445:452	arg1	focus					436:440	a focus	434:440	a focus of research	434:452	To improve repair, the incorporation of bionics into TEN grafts has become a focus of research.					
36871174	6	53	theme	mesenchymal	837:847	arg1	matrix					881:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	human bone mesenchymal stem cell-derived extracellular matrix	826:886	The lumen of the structure was filled with human bone mesenchymal stem cell-derived extracellular matrix and fibers to provide nutrition and topographic guidance, respectively.					
36871174	0	54	theme	Novel	62:66	arg1	Engineering					82:92	Novel Bionic Tissue Engineering	62:92	Novel Bionic Tissue Engineering	62:92	Experimental Study on Repairing Peripheral Nerve Defects with Novel Bionic Tissue Engineering.					
36871174	4	55	theme	novel	472:476	arg1	graft					489:493	a novel bionic TEN graft	470:493	a novel bionic TEN graft with a biomimetic structure and composition	470:537	In this study, a novel bionic TEN graft with a biomimetic structure and composition was designed.					
36871174	8	56	theme	autografts	1146:1155	arg1	similar					1162:1168	similar	1162:1168	similar	1162:1168	Morphological and functional examination showed that the repair effects of the TEN grafts and autografts were similar.					
36871174	8	56	theme	autografts	1146:1155	arg1	effects					1116:1122	the repair effects	1105:1122	the repair effects of the TEN grafts and autografts	1105:1155	Morphological and functional examination showed that the repair effects of the TEN grafts and autografts were similar.					
36986193	4	0	theme	grouped	568:574	arg1	HMOs					588:591	grouped fucosylated HMOs	568:591	grouped fucosylated HMOs	568:591	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	7	1	theme	age	1069:1071	arg1	years					1079:1083	age three years	1069:1083	age three years with two executive function questionnaires independently filled in by mothers and their partners, and four behavioural tasks	1069:1208	Executive functions were assessed at age three years with two executive function questionnaires independently filled in by mothers and their partners, and four behavioural tasks.					
36986193	2	2	theme	better	314:319	arg1	outcomes					331:338	better cognitive outcomes	314:338	better cognitive outcomes in the offspring	314:355	Animal studies have confirmed a link between early life exposure to HMOs and better cognitive outcomes in the offspring.					
36986193	9	3	theme	formula-fed	1650:1660	arg1	infants					1662:1668	exclusively formula-fed infants	1638:1668	exclusively formula-fed infants	1638:1668	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	2	4	from	exposure	293:300	arg1	offspring					347:355	the offspring	343:355	the offspring	343:355	Animal studies have confirmed a link between early life exposure to HMOs and better cognitive outcomes in the offspring.					
36986193	3	5	with	studies	364:370	arg1	cognition					414:422	later child cognition	402:422	later child cognition	402:422	Human studies on HMOs and associations with later child cognition are scarce.					
36986193	4	6	theme	first	643:647	arg1	weeks					666:670	the first twelve postnatal weeks	639:670	the first twelve postnatal weeks	639:670	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	8	7	gly	sialylated	1430:1439	arg1	HMOs					1441:1444	grouped sialylated HMOs	1422:1444	grouped sialylated HMOs	1422:1444	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	4	8	theme	postnatal	656:664	arg1	weeks					666:670	the first twelve postnatal weeks	639:670	the first twelve postnatal weeks	639:670	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	2	9	theme	early	282:286	arg1	exposure					293:300	early life exposure	282:300	early life exposure to HMOs	282:308	Animal studies have confirmed a link between early life exposure to HMOs and better cognitive outcomes in the offspring.					
36986193	2	10	from	outcomes	331:338	arg1	offspring					347:355	the offspring	343:355	the offspring	343:355	Animal studies have confirmed a link between early life exposure to HMOs and better cognitive outcomes in the offspring.					
36986193	8	11	theme	Multiple	1211:1218	arg1	analyses					1231:1238	Multiple regression analyses	1211:1238	Multiple regression analyses	1211:1238	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	1	12	theme	most	186:189	arg1	components					206:215	the most abundant solid components	182:215	the most abundant solid components in a mother's milk	182:234	Human milk oligosaccharides (HMOs) are one of the most abundant solid components in a mother's milk.					
36986193	9	13	theme	cognitive	1713:1721	arg1	development					1723:1733	child cognitive development	1707:1733	child cognitive development	1707:1733	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	8	14	theme	grouped	1422:1428	arg1	HMOs					1441:1444	grouped sialylated HMOs	1422:1444	grouped sialylated HMOs	1422:1444	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	1	15	theme	solid	200:204	arg1	components					206:215	the most abundant solid components	182:215	the most abundant solid components in a mother's milk	182:234	Human milk oligosaccharides (HMOs) are one of the most abundant solid components in a mother's milk.					
36986193	8	16	theme	HMOs	1441:1444	arg1	concentrations					1404:1417	concentrations	1404:1417	concentrations of grouped sialylated HMOs	1404:1444	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	4	17	theme	better	693:698	arg1	functions					716:724	better child executive functions	693:724	better child executive functions at age three years	693:743	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	9	18	theme	potential	1747:1755	arg1	causality					1757:1765	potential causality	1747:1765	potential causality	1747:1765	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	9	19	from	studies	1520:1526	arg1	infants					1662:1668	exclusively formula-fed infants	1638:1668	exclusively formula-fed infants	1638:1668	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	9	19	from	studies	1520:1526	arg1	HMOs					1531:1534	HMOs	1531:1534	HMOs that sample frequently during the first months of life	1531:1589	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	5	20	dep	breastfeeding	879:891	arg1	n					894:894	n = 18	894:899	n = 18	894:899	At infant age two, six, and twelve weeks, a sample of human milk was collected by mothers who were exclusively (n = 45) or partially breastfeeding (n = 18).					
36986193	1	21	theme	Human	136:140	arg1	oligosaccharides					147:162	Human milk oligosaccharides	136:162	Human milk oligosaccharides (HMOs)	136:169	Human milk oligosaccharides (HMOs) are one of the most abundant solid components in a mother's milk.					
36986193	1	21	theme	Human	136:140	arg1	HMOs					165:168	HMOs	165:168	HMOs	165:168	Human milk oligosaccharides (HMOs) are one of the most abundant solid components in a mother's milk.					
36986193	4	22	theme	executive	706:714	arg1	functions					716:724	better child executive functions	693:724	better child executive functions at age three years	693:743	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	8	23	theme	worse	1467:1471	arg1	functions					1483:1491	worse executive functions	1467:1491	worse executive functions at age three years	1467:1510	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	5	24	dep	or	866:867	arg1	n					858:858	n = 45	858:863	n = 45	858:863	At infant age two, six, and twelve weeks, a sample of human milk was collected by mothers who were exclusively (n = 45) or partially breastfeeding (n = 18).					
36986193	0	25	theme	Postnatal	60:68	arg1	Weeks					70:74	the First 12 Postnatal Weeks	47:74	the First 12 Postnatal Weeks	47:74	Fucosylated Human Milk Oligosaccharides during the First 12 Postnatal Weeks Are Associated with Better Executive Functions in Toddlers.					
36986193	3	26	from	studies	364:370	arg1	associations					384:395	associations	384:395	associations	384:395	Human studies on HMOs and associations with later child cognition are scarce.					
36986193	3	26	from	studies	364:370	arg1	HMOs					375:378	HMOs	375:378	HMOs	375:378	Human studies on HMOs and associations with later child cognition are scarce.					
36986193	7	27	theme	function	1104:1111	arg1	questionnaires					1113:1126	two executive function questionnaires	1090:1126	two executive function questionnaires independently filled in by mothers and their partners, and four behavioural tasks	1090:1208	Executive functions were assessed at age three years with two executive function questionnaires independently filled in by mothers and their partners, and four behavioural tasks.					
36986193	5	28	theme	infant	749:754	arg1	age					756:758	infant age	749:758	infant age	749:758	At infant age two, six, and twelve weeks, a sample of human milk was collected by mothers who were exclusively (n = 45) or partially breastfeeding (n = 18).					
36986193	5	29	from	age	756:758	arg1	weeks					781:785	two, six, and twelve weeks	760:785	two, six, and twelve weeks	760:785	At infant age two, six, and twelve weeks, a sample of human milk was collected by mothers who were exclusively (n = 45) or partially breastfeeding (n = 18).					
36986193	8	30	theme	age	1496:1498	arg1	years					1506:1510	age three years	1496:1510	age three years	1496:1510	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	8	31	theme	2'-fucosyllactose	1302:1318	arg1	concentrations					1284:1297	concentrations	1284:1297	concentrations of 2'-fucosyllactose and grouped fucosylated HMOs	1284:1347	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	5	32	theme	=	860:860	arg1	n					858:858	n = 45	858:863	n = 45	858:863	At infant age two, six, and twelve weeks, a sample of human milk was collected by mothers who were exclusively (n = 45) or partially breastfeeding (n = 18).					
36986193	8	33	theme	grouped	1324:1330	arg1	HMOs					1344:1347	grouped fucosylated HMOs	1324:1347	grouped fucosylated HMOs	1324:1347	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	3	34	theme	later	402:406	arg1	cognition					414:422	later child cognition	402:422	later child cognition	402:422	Human studies on HMOs and associations with later child cognition are scarce.					
36986193	0	35	theme	Executive	103:111	arg1	Functions					113:121	Better Executive Functions	96:121	Better Executive Functions in Toddlers	96:133	Fucosylated Human Milk Oligosaccharides during the First 12 Postnatal Weeks Are Associated with Better Executive Functions in Toddlers.					
36986193	5	36	theme	=	896:896	arg1	n					894:894	n = 18	894:899	n = 18	894:899	At infant age two, six, and twelve weeks, a sample of human milk was collected by mothers who were exclusively (n = 45) or partially breastfeeding (n = 18).					
36986193	6	37	theme	liquid	991:996	arg1	spectrometry					1018:1029	porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	942:1029	porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	942:1029	HMO composition was analysed by use of porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry.					
36986193	4	38	theme	preregistered	444:456	arg1	study					471:475	this preregistered longitudinal study	439:475	this preregistered longitudinal study	439:475	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	8	39	theme	HMOs	1344:1347	arg1	concentrations					1284:1297	concentrations	1284:1297	concentrations of 2'-fucosyllactose and grouped fucosylated HMOs	1284:1347	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	0	40	theme	Fucosylated	0:10	arg1	Oligosaccharides					23:38	Fucosylated Human Milk Oligosaccharides	0:38	Fucosylated Human Milk Oligosaccharides during the First 12 Postnatal Weeks	0:74	Fucosylated Human Milk Oligosaccharides during the First 12 Postnatal Weeks Are Associated with Better Executive Functions in Toddlers.					
36986193	4	41	gly	fucosylated	576:586	arg1	HMOs					588:591	grouped fucosylated HMOs	568:591	grouped fucosylated HMOs	568:591	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	6	42	theme	carbon-ultra	961:972	arg1	spectrometry					1018:1029	porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	942:1029	porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	942:1029	HMO composition was analysed by use of porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry.					
36986193	0	43	theme	Milk	18:21	arg1	Oligosaccharides					23:38	Fucosylated Human Milk Oligosaccharides	0:38	Fucosylated Human Milk Oligosaccharides during the First 12 Postnatal Weeks	0:74	Fucosylated Human Milk Oligosaccharides during the First 12 Postnatal Weeks Are Associated with Better Executive Functions in Toddlers.					
36986193	9	44	theme	first	1570:1574	arg1	months					1576:1581	the first months	1566:1581	the first months of life	1566:1589	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	6	45	theme	porous	942:947	arg1	spectrometry					1018:1029	porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	942:1029	porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	942:1029	HMO composition was analysed by use of porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry.					
36986193	8	46	theme	better	1370:1375	arg1	functions					1387:1395	better executive functions	1370:1395	better executive functions	1370:1395	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	8	47	gly	fucosylated	1332:1342	arg1	HMOs					1344:1347	grouped fucosylated HMOs	1324:1347	grouped fucosylated HMOs	1324:1347	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	8	48	from	years	1506:1510	arg1	functions					1483:1491	worse executive functions	1467:1491	worse executive functions at age three years	1467:1510	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	4	49	theme	milk	508:511	arg1	2'-fucosyllactose					513:529	human milk 2'-fucosyllactose	502:529	human milk 2'-fucosyllactose	502:529	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	1	50	theme	components	206:215	arg1	one					175:177	one	175:177	one	175:177	Human milk oligosaccharides (HMOs) are one of the most abundant solid components in a mother's milk.					
36986193	1	50	theme	components	206:215	arg1	components					206:215	the most abundant solid components	182:215	the most abundant solid components in a mother's milk	182:234	Human milk oligosaccharides (HMOs) are one of the most abundant solid components in a mother's milk.					
36986193	9	51	theme	HMO	1608:1610	arg1	studies					1627:1633	experimental HMO administration studies	1595:1633	experimental HMO administration studies in exclusively formula-fed infants	1595:1668	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	5	52	theme	milk	806:809	arg1	sample					790:795	a sample	788:795	a sample of human milk	788:809	At infant age two, six, and twelve weeks, a sample of human milk was collected by mothers who were exclusively (n = 45) or partially breastfeeding (n = 18).					
36986193	4	53	theme	grouped	598:604	arg1	HMOs					617:620	grouped sialylated HMOs	598:620	grouped sialylated HMOs	598:620	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	2	54	theme	cognitive	321:329	arg1	outcomes					331:338	better cognitive outcomes	314:338	better cognitive outcomes in the offspring	314:355	Animal studies have confirmed a link between early life exposure to HMOs and better cognitive outcomes in the offspring.					
36986193	6	55	theme	spectrometry	1018:1029	arg1	use					935:937	use	935:937	use of porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	935:1029	HMO composition was analysed by use of porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry.					
36986193	7	56	with	years	1079:1083	arg1	questionnaires					1113:1126	two executive function questionnaires	1090:1126	two executive function questionnaires independently filled in by mothers and their partners, and four behavioural tasks	1090:1208	Executive functions were assessed at age three years with two executive function questionnaires independently filled in by mothers and their partners, and four behavioural tasks.					
36986193	4	57	theme	fucosylated	576:586	arg1	HMOs					588:591	grouped fucosylated HMOs	568:591	grouped fucosylated HMOs	568:591	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	7	58	theme	Executive	1032:1040	arg1	functions					1042:1050	Executive functions	1032:1050	Executive functions	1032:1050	Executive functions were assessed at age three years with two executive function questionnaires independently filled in by mothers and their partners, and four behavioural tasks.					
36986193	5	59	theme	human	800:804	arg1	milk					806:809	human milk	800:809	human milk	800:809	At infant age two, six, and twelve weeks, a sample of human milk was collected by mothers who were exclusively (n = 45) or partially breastfeeding (n = 18).					
36986193	0	60	theme	First	51:55	arg1	Weeks					70:74	the First 12 Postnatal Weeks	47:74	the First 12 Postnatal Weeks	47:74	Fucosylated Human Milk Oligosaccharides during the First 12 Postnatal Weeks Are Associated with Better Executive Functions in Toddlers.					
36986193	2	61	theme	life	288:291	arg1	exposure					293:300	early life exposure	282:300	early life exposure to HMOs	282:308	Animal studies have confirmed a link between early life exposure to HMOs and better cognitive outcomes in the offspring.					
36986193	9	62	theme	child	1707:1711	arg1	development					1723:1733	child cognitive development	1707:1733	child cognitive development	1707:1733	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	8	63	theme	sialylated	1430:1439	arg1	HMOs					1441:1444	grouped sialylated HMOs	1422:1444	grouped sialylated HMOs	1422:1444	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	1	64	theme	abundant	191:198	arg1	components					206:215	the most abundant solid components	182:215	the most abundant solid components in a mother's milk	182:234	Human milk oligosaccharides (HMOs) are one of the most abundant solid components in a mother's milk.					
36986193	8	65	theme	regression	1220:1229	arg1	analyses					1231:1238	Multiple regression analyses	1211:1238	Multiple regression analyses	1211:1238	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	0	66	from	Functions	113:121	arg1	Toddlers					126:133	Toddlers	126:133	Toddlers	126:133	Fucosylated Human Milk Oligosaccharides during the First 12 Postnatal Weeks Are Associated with Better Executive Functions in Toddlers.					
36986193	1	67	from	components	206:215	arg1	milk					231:234	a mother's milk	220:234	a mother's milk	220:234	Human milk oligosaccharides (HMOs) are one of the most abundant solid components in a mother's milk.					
36986193	9	68	theme	experimental	1595:1606	arg1	studies					1627:1633	experimental HMO administration studies	1595:1633	experimental HMO administration studies in exclusively formula-fed infants	1595:1668	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	2	69	theme	Animal	237:242	arg1	studies					244:250	Animal studies	237:250	Animal studies	237:250	Animal studies have confirmed a link between early life exposure to HMOs and better cognitive outcomes in the offspring.					
36986193	8	70	theme	executive	1473:1481	arg1	functions					1483:1491	worse executive functions	1467:1491	worse executive functions at age three years	1467:1510	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	4	71	theme	child	700:704	arg1	functions					716:724	better child executive functions	693:724	better child executive functions at age three years	693:743	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	1	72	theme	milk	142:145	arg1	oligosaccharides					147:162	Human milk oligosaccharides	136:162	Human milk oligosaccharides (HMOs)	136:169	Human milk oligosaccharides (HMOs) are one of the most abundant solid components in a mother's milk.					
36986193	1	72	theme	milk	142:145	arg1	HMOs					165:168	HMOs	165:168	HMOs	165:168	Human milk oligosaccharides (HMOs) are one of the most abundant solid components in a mother's milk.					
36986193	9	73	theme	sensitive	1771:1779	arg1	periods					1781:1787	sensitive periods	1771:1787	sensitive periods	1771:1787	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	9	74	theme	Future	1513:1518	arg1	studies					1520:1526	Future studies	1513:1526	Future studies on HMOs that sample frequently during the first months of life	1513:1589	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	6	75	theme	HMO	903:905	arg1	composition					907:917	HMO composition	903:917	HMO composition	903:917	HMO composition was analysed by use of porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry.					
36986193	4	76	gly	sialylated	606:615	arg1	HMOs					617:620	grouped sialylated HMOs	598:620	grouped sialylated HMOs	598:620	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	9	77	from	studies	1627:1633	arg1	infants					1662:1668	exclusively formula-fed infants	1638:1668	exclusively formula-fed infants	1638:1668	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	9	77	from	studies	1627:1633	arg1	HMOs					1531:1534	HMOs	1531:1534	HMOs that sample frequently during the first months of life	1531:1589	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	6	78	theme	chromatography-mass	998:1016	arg1	spectrometry					1018:1029	porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	942:1029	porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	942:1029	HMO composition was analysed by use of porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry.					
36986193	4	79	theme	longitudinal	458:469	arg1	study					471:475	this preregistered longitudinal study	439:475	this preregistered longitudinal study	439:475	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	3	80	theme	child	408:412	arg1	cognition					414:422	later child cognition	402:422	later child cognition	402:422	Human studies on HMOs and associations with later child cognition are scarce.					
36986193	0	81	theme	Human	12:16	arg1	Oligosaccharides					23:38	Fucosylated Human Milk Oligosaccharides	0:38	Fucosylated Human Milk Oligosaccharides during the First 12 Postnatal Weeks	0:74	Fucosylated Human Milk Oligosaccharides during the First 12 Postnatal Weeks Are Associated with Better Executive Functions in Toddlers.					
36986193	6	82	theme	high-performance	974:989	arg1	spectrometry					1018:1029	porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	942:1029	porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	942:1029	HMO composition was analysed by use of porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry.					
36986193	3	83	theme	Human	358:362	arg1	scarce					428:433	scarce	428:433	scarce	428:433	Human studies on HMOs and associations with later child cognition are scarce.					
36986193	3	83	theme	Human	358:362	arg1	studies					364:370	Human studies	358:370	Human studies on HMOs and associations with later child cognition	358:422	Human studies on HMOs and associations with later child cognition are scarce.					
36986193	8	84	theme	fucosylated	1332:1342	arg1	HMOs					1344:1347	grouped fucosylated HMOs	1324:1347	grouped fucosylated HMOs	1324:1347	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	7	85	theme	executive	1094:1102	arg1	questionnaires					1113:1126	two executive function questionnaires	1090:1126	two executive function questionnaires independently filled in by mothers and their partners, and four behavioural tasks	1090:1208	Executive functions were assessed at age three years with two executive function questionnaires independently filled in by mothers and their partners, and four behavioural tasks.					
36986193	4	86	from	years	739:743	arg1	functions					716:724	better child executive functions	693:724	better child executive functions at age three years	693:743	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	6	87	theme	graphitized	949:959	arg1	spectrometry					1018:1029	porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	942:1029	porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry	942:1029	HMO composition was analysed by use of porous graphitized carbon-ultra high-performance liquid chromatography-mass spectrometry.					
36986193	4	88	theme	age	729:731	arg1	years					739:743	age three years	729:743	age three years	729:743	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	4	89	theme	human	502:506	arg1	2'-fucosyllactose					513:529	human milk 2'-fucosyllactose	502:529	human milk 2'-fucosyllactose	502:529	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
36986193	9	90	theme	life	1586:1589	arg1	months					1576:1581	the first months	1566:1581	the first months of life	1566:1589	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	8	91	theme	executive	1377:1385	arg1	functions					1387:1395	better executive functions	1370:1395	better executive functions	1370:1395	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	9	92	with	associations	1689:1700	arg1	development					1723:1733	child cognitive development	1707:1733	child cognitive development	1707:1733	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	8	93	dep	R.	1258:1259	arg1	indicated					1269:1277	indicated	1269:1277	indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years	1269:1510	Multiple regression analyses were performed in R. Results indicated that concentrations of 2'-fucosyllactose and grouped fucosylated HMOs were associated with better executive functions, while concentrations of grouped sialylated HMOs were associated with worse executive functions at age three years.					
36986193	9	94	theme	administration	1612:1625	arg1	studies					1627:1633	experimental HMO administration studies	1595:1633	experimental HMO administration studies in exclusively formula-fed infants	1595:1668	Future studies on HMOs that sample frequently during the first months of life and experimental HMO administration studies in exclusively formula-fed infants can further reveal associations with child cognitive development and uncover potential causality and sensitive periods.					
36986193	7	95	theme	behavioural	1192:1202	arg1	tasks					1204:1208	four behavioural tasks	1187:1208	four behavioural tasks	1187:1208	Executive functions were assessed at age three years with two executive function questionnaires independently filled in by mothers and their partners, and four behavioural tasks.					
36986193	4	96	theme	sialylated	606:615	arg1	HMOs					617:620	grouped sialylated HMOs	598:620	grouped sialylated HMOs	598:620	In this preregistered longitudinal study, we investigated whether human milk 2'-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, grouped fucosylated HMOs, and grouped sialylated HMOs, assessed during the first twelve postnatal weeks, are associated with better child executive functions at age three years.					
35322314	12	0	theme	individuals	1345:1355	arg1	proportion					1331:1340	The proportion	1327:1340	The proportion of individuals with abnormal lactase activity	1327:1386	The proportion of individuals with abnormal lactase activity did not differ by lactose intolerance status.					
35322314	9	1	theme	composition	1055:1065	arg1	associations					1017:1028	associations	1017:1028	associations of small bowel microbial composition with self-reported lactose intolerance and symptoms	1017:1117	We examined associations of small bowel microbial composition with self-reported lactose intolerance and symptoms.					
35322314	15	2	theme	beta	1710:1713	arg1	diversity					1715:1723	small intestinal microbiome beta diversity	1682:1723	small intestinal microbiome beta diversity	1682:1723	In 19 patients, we observed significant associations of small intestinal microbiome beta diversity with stool consistency after consuming dairy (p = 0.03).					
35322314	14	3	theme	lactose	1580:1586	arg1	intake					1588:1593	Daily lactose intake	1574:1593	Daily lactose intake	1574:1593	Daily lactose intake did not differ between groups.					
35322314	9	4	theme	self-reported	1072:1084	arg1	intolerance					1094:1104	self-reported lactose intolerance	1072:1104	self-reported lactose intolerance	1072:1104	We examined associations of small bowel microbial composition with self-reported lactose intolerance and symptoms.					
35322314	15	5	theme	intestinal	1688:1697	arg1	diversity					1715:1723	small intestinal microbiome beta diversity	1682:1723	small intestinal microbiome beta diversity	1682:1723	In 19 patients, we observed significant associations of small intestinal microbiome beta diversity with stool consistency after consuming dairy (p = 0.03).					
35322314	1	6	theme	lactose	274:280	arg1	intolerance					282:292	lactose intolerance	274:292	lactose intolerance	274:292	BACKGROUND Approximately two-thirds of adults are genetically predisposed to decreased lactase activity after weaning, putting them at risk of lactose intolerance.					
35322314	11	7	theme	lactose	1197:1203	arg1	intolerance					1205:1215	lactose intolerance	1197:1215	lactose intolerance	1197:1215	Those with lactose intolerance had higher total symptom scores, more frequent bowel urgency, and more bowel movements after consuming dairy.					
35322314	16	8	theme	Intestinal	1794:1803	arg1	lactase					1805:1811	CONCLUSIONS Intestinal lactase	1782:1811	CONCLUSIONS Intestinal lactase	1782:1811	CONCLUSIONS Intestinal lactase is associated with lactose intolerance symptoms and bowel urgency in adults but does not distinguish the clinical phenotype entirely.					
35322314	1	9	theme	lactase	218:224	arg1	activity					226:233	decreased lactase activity	208:233	decreased lactase activity	208:233	BACKGROUND Approximately two-thirds of adults are genetically predisposed to decreased lactase activity after weaning, putting them at risk of lactose intolerance.					
35322314	0	10	from	Symptoms	77:84	arg1	Adults					123:128	Adults	123:128	Adults	123:128	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	15	11	with	associations	1666:1677	arg1	consistency					1736:1746	stool consistency	1730:1746	stool consistency	1730:1746	In 19 patients, we observed significant associations of small intestinal microbiome beta diversity with stool consistency after consuming dairy (p = 0.03).					
35322314	13	12	theme	intolerance	1491:1501	arg1	scores					1511:1516	total lactose intolerance symptom scores	1477:1516	total lactose intolerance symptom scores (p = 0.038)	1477:1528	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	13	12	theme	intolerance	1491:1501	arg1	p = 0.038					1519:1527	p = 0.038	1519:1527	p = 0.038	1519:1527	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	5	13	theme	study	608:612	arg1	participants					614:625	Observational study participants	594:625	Observational study participants	594:625	Observational study participants completed a lactose intolerance symptom questionnaire and reported lactose intake.					
35322314	8	14	theme	lactose	944:950	arg1	intolerance					952:962	lactose intolerance	944:962	lactose intolerance symptoms	944:971	We assessed associations between lactose intolerance symptoms and lactase and lactose intake.					
35322314	13	15	theme	total	1477:1481	arg1	scores					1511:1516	total lactose intolerance symptom scores	1477:1516	total lactose intolerance symptom scores (p = 0.038)	1477:1528	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	13	15	theme	total	1477:1481	arg1	p = 0.038					1519:1527	p = 0.038	1519:1527	p = 0.038	1519:1527	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	16	16	theme	clinical	1918:1925	arg1	phenotype					1927:1935	the clinical phenotype	1914:1935	the clinical phenotype	1914:1935	CONCLUSIONS Intestinal lactase is associated with lactose intolerance symptoms and bowel urgency in adults but does not distinguish the clinical phenotype entirely.					
35322314	13	17	theme	Median	1434:1439	arg1	levels					1449:1454	Median lactase levels	1434:1454	Median lactase levels	1434:1454	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	5	18	theme	symptom	659:665	arg1	questionnaire					667:679	a lactose intolerance symptom questionnaire	637:679	a lactose intolerance symptom questionnaire	637:679	Observational study participants completed a lactose intolerance symptom questionnaire and reported lactose intake.					
35322314	1	19	theme	adults	170:175	arg1	BACKGROUND					131:140	BACKGROUND Approximately two-thirds	131:165	BACKGROUND Approximately two-thirds of adults	131:175	BACKGROUND Approximately two-thirds of adults are genetically predisposed to decreased lactase activity after weaning, putting them at risk of lactose intolerance.					
35322314	0	20	theme	Lactose	89:95	arg1	Intolerance					97:107	Lactose Intolerance	89:107	Lactose Intolerance	89:107	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	8	21	theme	lactose	989:995	arg1	intake					997:1002	lactose intake	989:1002	lactose intake	989:1002	We assessed associations between lactose intolerance symptoms and lactase and lactose intake.					
35322314	6	22	theme	small	788:792	arg1	microbiome					813:822	the small intestinal mucosal microbiome	784:822	the small intestinal mucosal microbiome	784:822	Post-bulbar biopsies were obtained to measure lactase activity and assess the small intestinal mucosal microbiome.					
35322314	12	23	theme	lactose	1406:1412	arg1	status					1426:1431	lactose intolerance status	1406:1431	lactose intolerance status	1406:1431	The proportion of individuals with abnormal lactase activity did not differ by lactose intolerance status.					
35322314	3	24	theme	self-reported	393:405	arg1	intolerance					415:425	self-reported lactose intolerance	393:425	self-reported lactose intolerance	393:425	AIMS We assessed association between self-reported lactose intolerance and intestinal lactase, lactose intake, and the small intestinal microbiome.					
35322314	4	25	theme	METHODS	504:510	arg1	18-75 years					521:531	METHODS Patients 18-75 years	504:531	METHODS Patients 18-75 years presenting for upper endoscopy	504:562	METHODS Patients 18-75 years presenting for upper endoscopy were recruited prospectively.					
35322314	1	26	theme	intolerance	282:292	arg1	risk					266:269	risk	266:269	risk of lactose intolerance	266:292	BACKGROUND Approximately two-thirds of adults are genetically predisposed to decreased lactase activity after weaning, putting them at risk of lactose intolerance.					
35322314	17	27	theme	intolerance	2053:2063	arg1	pathophysiology					2026:2040	the pathophysiology	2022:2040	the pathophysiology of lactose intolerance	2022:2063	Studying other contributing factors (microbiota, diet) may further clarify the pathophysiology of lactose intolerance.					
35322314	5	28	theme	lactose	639:645	arg1	questionnaire					667:679	a lactose intolerance symptom questionnaire	637:679	a lactose intolerance symptom questionnaire	637:679	Observational study participants completed a lactose intolerance symptom questionnaire and reported lactose intake.					
35322314	16	29	from	urgency	1871:1877	arg1	adults					1882:1887	adults	1882:1887	adults	1882:1887	CONCLUSIONS Intestinal lactase is associated with lactose intolerance symptoms and bowel urgency in adults but does not distinguish the clinical phenotype entirely.					
35322314	3	30	theme	small	475:479	arg1	microbiome					492:501	the small intestinal microbiome	471:501	the small intestinal microbiome	471:501	AIMS We assessed association between self-reported lactose intolerance and intestinal lactase, lactose intake, and the small intestinal microbiome.					
35322314	0	31	theme	Intestinal	17:26	arg1	Lactase					28:34	Intestinal Lactase	17:34	Intestinal Lactase	17:34	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	7	32	theme	lactose	890:896	arg1	intolerance					898:908	lactose intolerance	890:908	lactose intolerance	890:908	We compared intestinal lactase between patients with and without lactose intolerance.					
35322314	17	33	theme	contributing	1962:1973	arg1	factors					1975:1981	other contributing factors	1956:1981	other contributing factors (microbiota, diet)	1956:2000	Studying other contributing factors (microbiota, diet) may further clarify the pathophysiology of lactose intolerance.					
35322314	16	34	dep	intolerance	1840:1850	arg1	symptoms					1852:1859	symptoms	1852:1859	symptoms	1852:1859	CONCLUSIONS Intestinal lactase is associated with lactose intolerance symptoms and bowel urgency in adults but does not distinguish the clinical phenotype entirely.					
35322314	3	35	theme	intestinal	431:440	arg1	lactase					442:448	intestinal lactase	431:448	intestinal lactase	431:448	AIMS We assessed association between self-reported lactose intolerance and intestinal lactase, lactose intake, and the small intestinal microbiome.					
35322314	2	36	theme	maldigestion	342:353	arg1	marker					324:329	a poor marker	317:329	a poor marker of lactose maldigestion	317:353	However, symptoms are a poor marker of lactose maldigestion.					
35322314	2	36	theme	maldigestion	342:353	arg1	symptoms					304:311	symptoms	304:311	symptoms	304:311	However, symptoms are a poor marker of lactose maldigestion.					
35322314	0	37	theme	Small	44:48	arg1	Microbiome					61:70	the Small Intestinal Microbiome	40:70	the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults	40:128	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	0	38	with	Relationships	0:12	arg1	Symptoms					77:84	Symptoms	77:84	Symptoms of Lactose Intolerance	77:107	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	0	38	with	Relationships	0:12	arg1	Intake					113:118	Intake	113:118	Intake in Adults	113:128	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	12	39	theme	lactase	1371:1377	arg1	activity					1379:1386	abnormal lactase activity	1362:1386	abnormal lactase activity	1362:1386	The proportion of individuals with abnormal lactase activity did not differ by lactose intolerance status.					
35322314	9	40	theme	small	1033:1037	arg1	composition					1055:1065	small bowel microbial composition	1033:1065	small bowel microbial composition	1033:1065	We examined associations of small bowel microbial composition with self-reported lactose intolerance and symptoms.					
35322314	11	41	theme	bowel	1264:1268	arg1	urgency					1270:1276	more frequent bowel urgency	1250:1276	more frequent bowel urgency	1250:1276	Those with lactose intolerance had higher total symptom scores, more frequent bowel urgency, and more bowel movements after consuming dairy.					
35322314	9	42	theme	microbial	1045:1053	arg1	composition					1055:1065	small bowel microbial composition	1033:1065	small bowel microbial composition	1033:1065	We examined associations of small bowel microbial composition with self-reported lactose intolerance and symptoms.					
35322314	15	43	theme	stool	1730:1734	arg1	consistency					1736:1746	stool consistency	1730:1746	stool consistency	1730:1746	In 19 patients, we observed significant associations of small intestinal microbiome beta diversity with stool consistency after consuming dairy (p = 0.03).					
35322314	11	44	contain	had	1217:1219	arg2	urgency					1270:1276	more frequent bowel urgency	1250:1276	more frequent bowel urgency	1250:1276	Those with lactose intolerance had higher total symptom scores, more frequent bowel urgency, and more bowel movements after consuming dairy.					
35322314	11	44	contain	had	1217:1219	arg2	movements					1294:1302	more bowel movements	1283:1302	more bowel movements	1283:1302	Those with lactose intolerance had higher total symptom scores, more frequent bowel urgency, and more bowel movements after consuming dairy.					
35322314	11	44	contain	had	1217:1219	arg1	Those					1186:1190	Those	1186:1190	Those	1186:1190	Those with lactose intolerance had higher total symptom scores, more frequent bowel urgency, and more bowel movements after consuming dairy.					
35322314	11	44	contain	had	1217:1219	arg2	scores					1242:1247	higher total symptom scores	1221:1247	higher total symptom scores	1221:1247	Those with lactose intolerance had higher total symptom scores, more frequent bowel urgency, and more bowel movements after consuming dairy.					
35322314	6	45	theme	mucosal	805:811	arg1	microbiome					813:822	the small intestinal mucosal microbiome	784:822	the small intestinal mucosal microbiome	784:822	Post-bulbar biopsies were obtained to measure lactase activity and assess the small intestinal mucosal microbiome.					
35322314	13	46	theme	urgency	1553:1559	arg1	scores					1511:1516	total lactose intolerance symptom scores	1477:1516	total lactose intolerance symptom scores (p = 0.038)	1477:1528	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	13	46	theme	urgency	1553:1559	arg1	p = 0.038					1519:1527	p = 0.038	1519:1527	p = 0.038	1519:1527	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	13	46	theme	urgency	1553:1559	arg1	frequency					1534:1542	frequency	1534:1542	frequency of bowel urgency (p = 0.012)	1534:1571	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	16	47	from	intolerance	1840:1850	arg1	adults					1882:1887	adults	1882:1887	adults	1882:1887	CONCLUSIONS Intestinal lactase is associated with lactose intolerance symptoms and bowel urgency in adults but does not distinguish the clinical phenotype entirely.					
35322314	15	48	theme	diversity	1715:1723	arg1	associations					1666:1677	significant associations	1654:1677	significant associations of small intestinal microbiome beta diversity with stool consistency	1654:1746	In 19 patients, we observed significant associations of small intestinal microbiome beta diversity with stool consistency after consuming dairy (p = 0.03).					
35322314	14	49	theme	Daily	1574:1578	arg1	intake					1588:1593	Daily lactose intake	1574:1593	Daily lactose intake	1574:1593	Daily lactose intake did not differ between groups.					
35322314	9	50	theme	lactose	1086:1092	arg1	intolerance					1094:1104	self-reported lactose intolerance	1072:1104	self-reported lactose intolerance	1072:1104	We examined associations of small bowel microbial composition with self-reported lactose intolerance and symptoms.					
35322314	15	51	theme	microbiome	1699:1708	arg1	diversity					1715:1723	small intestinal microbiome beta diversity	1682:1723	small intestinal microbiome beta diversity	1682:1723	In 19 patients, we observed significant associations of small intestinal microbiome beta diversity with stool consistency after consuming dairy (p = 0.03).					
35322314	5	52	theme	lactose	694:700	arg1	intake					702:707	lactose intake	694:707	lactose intake	694:707	Observational study participants completed a lactose intolerance symptom questionnaire and reported lactose intake.					
35322314	0	53	with	Microbiome	61:70	arg1	Symptoms					77:84	Symptoms	77:84	Symptoms of Lactose Intolerance	77:107	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	0	53	with	Microbiome	61:70	arg1	Intake					113:118	Intake	113:118	Intake in Adults	113:128	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	9	54	with	associations	1017:1028	arg1	symptoms					1110:1117	symptoms	1110:1117	symptoms	1110:1117	We examined associations of small bowel microbial composition with self-reported lactose intolerance and symptoms.					
35322314	9	54	with	associations	1017:1028	arg1	intolerance					1094:1104	self-reported lactose intolerance	1072:1104	self-reported lactose intolerance	1072:1104	We examined associations of small bowel microbial composition with self-reported lactose intolerance and symptoms.					
35322314	15	55	theme	small	1682:1686	arg1	diversity					1715:1723	small intestinal microbiome beta diversity	1682:1723	small intestinal microbiome beta diversity	1682:1723	In 19 patients, we observed significant associations of small intestinal microbiome beta diversity with stool consistency after consuming dairy (p = 0.03).					
35322314	2	56	theme	poor	319:322	arg1	marker					324:329	a poor marker	317:329	a poor marker of lactose maldigestion	317:353	However, symptoms are a poor marker of lactose maldigestion.					
35322314	2	56	theme	poor	319:322	arg1	symptoms					304:311	symptoms	304:311	symptoms	304:311	However, symptoms are a poor marker of lactose maldigestion.					
35322314	11	57	theme	higher	1221:1226	arg1	scores					1242:1247	higher total symptom scores	1221:1247	higher total symptom scores	1221:1247	Those with lactose intolerance had higher total symptom scores, more frequent bowel urgency, and more bowel movements after consuming dairy.					
35322314	16	58	theme	CONCLUSIONS	1782:1792	arg1	lactase					1805:1811	CONCLUSIONS Intestinal lactase	1782:1811	CONCLUSIONS Intestinal lactase	1782:1811	CONCLUSIONS Intestinal lactase is associated with lactose intolerance symptoms and bowel urgency in adults but does not distinguish the clinical phenotype entirely.					
35322314	13	59	theme	symptom	1503:1509	arg1	scores					1511:1516	total lactose intolerance symptom scores	1477:1516	total lactose intolerance symptom scores (p = 0.038)	1477:1528	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	13	59	theme	symptom	1503:1509	arg1	p = 0.038					1519:1527	p = 0.038	1519:1527	p = 0.038	1519:1527	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	5	60	theme	Observational	594:606	arg1	participants					614:625	Observational study participants	594:625	Observational study participants	594:625	Observational study participants completed a lactose intolerance symptom questionnaire and reported lactose intake.					
35322314	0	61	from	Intake	113:118	arg1	Adults					123:128	Adults	123:128	Adults	123:128	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	10	62	theme	lactose	1165:1171	arg1	intolerance					1173:1183	lactose intolerance	1165:1183	lactose intolerance	1165:1183	RESULTS Among 34 patients, 23 (68%) reported lactose intolerance.					
35322314	17	63	dep	factors	1975:1981	arg1	diet					1996:1999	diet	1996:1999	diet	1996:1999	Studying other contributing factors (microbiota, diet) may further clarify the pathophysiology of lactose intolerance.					
35322314	17	63	dep	factors	1975:1981	arg1	microbiota					1984:1993	microbiota	1984:1993	microbiota	1984:1993	Studying other contributing factors (microbiota, diet) may further clarify the pathophysiology of lactose intolerance.					
35322314	16	64	theme	lactose	1832:1838	arg1	intolerance					1840:1850	lactose intolerance symptoms and bowel urgency	1832:1877	intolerance	1840:1850	CONCLUSIONS Intestinal lactase is associated with lactose intolerance symptoms and bowel urgency in adults but does not distinguish the clinical phenotype entirely.					
35322314	1	65	theme	decreased	208:216	arg1	activity					226:233	decreased lactase activity	208:233	decreased lactase activity	208:233	BACKGROUND Approximately two-thirds of adults are genetically predisposed to decreased lactase activity after weaning, putting them at risk of lactose intolerance.					
35322314	6	66	theme	Post-bulbar	710:720	arg1	biopsies					722:729	Post-bulbar biopsies	710:729	Post-bulbar biopsies	710:729	Post-bulbar biopsies were obtained to measure lactase activity and assess the small intestinal mucosal microbiome.					
35322314	13	67	theme	lactose	1483:1489	arg1	intolerance					1491:1501	lactose intolerance	1483:1501	total lactose intolerance symptom scores (p = 0.038)	1477:1528	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	8	68	theme	intolerance	952:962	arg1	symptoms					964:971	lactose intolerance symptoms	944:971	lactose intolerance symptoms	944:971	We assessed associations between lactose intolerance symptoms and lactase and lactose intake.					
35322314	4	69	theme	upper	548:552	arg1	endoscopy					554:562	upper endoscopy	548:562	upper endoscopy	548:562	METHODS Patients 18-75 years presenting for upper endoscopy were recruited prospectively.					
35322314	13	70	theme	lactase	1441:1447	arg1	levels					1449:1454	Median lactase levels	1434:1454	Median lactase levels	1434:1454	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	0	71	theme	Intolerance	97:107	arg1	Symptoms					77:84	Symptoms	77:84	Symptoms of Lactose Intolerance	77:107	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	0	71	theme	Intolerance	97:107	arg1	Intake					113:118	Intake	113:118	Intake in Adults	113:128	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	11	72	theme	bowel	1288:1292	arg1	movements					1294:1302	more bowel movements	1283:1302	more bowel movements	1283:1302	Those with lactose intolerance had higher total symptom scores, more frequent bowel urgency, and more bowel movements after consuming dairy.					
35322314	6	73	theme	intestinal	794:803	arg1	microbiome					813:822	the small intestinal mucosal microbiome	784:822	the small intestinal mucosal microbiome	784:822	Post-bulbar biopsies were obtained to measure lactase activity and assess the small intestinal mucosal microbiome.					
35322314	11	74	theme	total	1228:1232	arg1	scores					1242:1247	higher total symptom scores	1221:1247	higher total symptom scores	1221:1247	Those with lactose intolerance had higher total symptom scores, more frequent bowel urgency, and more bowel movements after consuming dairy.					
35322314	12	75	theme	intolerance	1414:1424	arg1	status					1426:1431	lactose intolerance status	1406:1431	lactose intolerance status	1406:1431	The proportion of individuals with abnormal lactase activity did not differ by lactose intolerance status.					
35322314	4	76	theme	Patients	512:519	arg1	18-75 years					521:531	METHODS Patients 18-75 years	504:531	METHODS Patients 18-75 years presenting for upper endoscopy	504:562	METHODS Patients 18-75 years presenting for upper endoscopy were recruited prospectively.					
35322314	12	77	with	individuals	1345:1355	arg1	activity					1379:1386	abnormal lactase activity	1362:1386	abnormal lactase activity	1362:1386	The proportion of individuals with abnormal lactase activity did not differ by lactose intolerance status.					
35322314	17	78	theme	lactose	2045:2051	arg1	intolerance					2053:2063	lactose intolerance	2045:2063	lactose intolerance	2045:2063	Studying other contributing factors (microbiota, diet) may further clarify the pathophysiology of lactose intolerance.					
35322314	3	79	theme	lactose	407:413	arg1	intolerance					415:425	self-reported lactose intolerance	393:425	self-reported lactose intolerance	393:425	AIMS We assessed association between self-reported lactose intolerance and intestinal lactase, lactose intake, and the small intestinal microbiome.					
35322314	3	80	theme	lactose	451:457	arg1	intake					459:464	lactose intake	451:464	lactose intake	451:464	AIMS We assessed association between self-reported lactose intolerance and intestinal lactase, lactose intake, and the small intestinal microbiome.					
35322314	0	81	theme	Lactase	28:34	arg1	Relationships					0:12	Relationships	0:12	Relationships of Intestinal Lactase	0:34	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	0	81	theme	Lactase	28:34	arg1	Microbiome					61:70	the Small Intestinal Microbiome	40:70	the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults	40:128	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	10	82	theme	34	1134:1135	arg1	patients					1137:1144	34 patients	1134:1144	34 patients	1134:1144	RESULTS Among 34 patients, 23 (68%) reported lactose intolerance.					
35322314	17	83	theme	other	1956:1960	arg1	factors					1975:1981	other contributing factors	1956:1981	other contributing factors (microbiota, diet)	1956:2000	Studying other contributing factors (microbiota, diet) may further clarify the pathophysiology of lactose intolerance.					
35322314	5	84	theme	intolerance	647:657	arg1	questionnaire					667:679	a lactose intolerance symptom questionnaire	637:679	a lactose intolerance symptom questionnaire	637:679	Observational study participants completed a lactose intolerance symptom questionnaire and reported lactose intake.					
35322314	15	85	theme	significant	1654:1664	arg1	associations					1666:1677	significant associations	1654:1677	significant associations of small intestinal microbiome beta diversity with stool consistency	1654:1746	In 19 patients, we observed significant associations of small intestinal microbiome beta diversity with stool consistency after consuming dairy (p = 0.03).					
35322314	6	86	theme	lactase	756:762	arg1	activity					764:771	lactase activity	756:771	lactase activity	756:771	Post-bulbar biopsies were obtained to measure lactase activity and assess the small intestinal mucosal microbiome.					
35322314	3	87	theme	intestinal	481:490	arg1	microbiome					492:501	the small intestinal microbiome	471:501	the small intestinal microbiome	471:501	AIMS We assessed association between self-reported lactose intolerance and intestinal lactase, lactose intake, and the small intestinal microbiome.					
35322314	10	88	dep	RESULTS	1120:1126	arg1	reported					1156:1163	reported	1156:1163	reported lactose intolerance	1156:1183	RESULTS Among 34 patients, 23 (68%) reported lactose intolerance.					
35322314	0	89	theme	Intestinal	50:59	arg1	Microbiome					61:70	the Small Intestinal Microbiome	40:70	the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults	40:128	Relationships of Intestinal Lactase and the Small Intestinal Microbiome with Symptoms of Lactose Intolerance and Intake in Adults.					
35322314	11	90	theme	symptom	1234:1240	arg1	scores					1242:1247	higher total symptom scores	1221:1247	higher total symptom scores	1221:1247	Those with lactose intolerance had higher total symptom scores, more frequent bowel urgency, and more bowel movements after consuming dairy.					
35322314	12	91	theme	abnormal	1362:1369	arg1	activity					1379:1386	abnormal lactase activity	1362:1386	abnormal lactase activity	1362:1386	The proportion of individuals with abnormal lactase activity did not differ by lactose intolerance status.					
35322314	3	92	dep	AIMS	356:359	arg1	assessed					364:371	assessed	364:371	assessed association between self-reported lactose intolerance and intestinal lactase, lactose intake, and the small intestinal microbiome	364:501	AIMS We assessed association between self-reported lactose intolerance and intestinal lactase, lactose intake, and the small intestinal microbiome.					
35322314	2	93	theme	lactose	334:340	arg1	maldigestion					342:353	lactose maldigestion	334:353	lactose maldigestion	334:353	However, symptoms are a poor marker of lactose maldigestion.					
35322314	11	94	theme	frequent	1255:1262	arg1	urgency					1270:1276	more frequent bowel urgency	1250:1276	more frequent bowel urgency	1250:1276	Those with lactose intolerance had higher total symptom scores, more frequent bowel urgency, and more bowel movements after consuming dairy.					
35322314	9	95	theme	bowel	1039:1043	arg1	composition					1055:1065	small bowel microbial composition	1033:1065	small bowel microbial composition	1033:1065	We examined associations of small bowel microbial composition with self-reported lactose intolerance and symptoms.					
35322314	16	96	theme	bowel	1865:1869	arg1	urgency					1871:1877	bowel urgency	1865:1877	bowel urgency	1865:1877	CONCLUSIONS Intestinal lactase is associated with lactose intolerance symptoms and bowel urgency in adults but does not distinguish the clinical phenotype entirely.					
35322314	13	97	theme	bowel	1547:1551	arg1	urgency					1553:1559	bowel urgency	1547:1559	bowel urgency (p = 0.012)	1547:1571	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	13	97	theme	bowel	1547:1551	arg1	p = 0.012					1562:1570	p = 0.012	1562:1570	p = 0.012	1562:1570	Median lactase levels were correlated with total lactose intolerance symptom scores (p = 0.038) and frequency of bowel urgency (p = 0.012).					
35322314	7	98	theme	intestinal	837:846	arg1	lactase					848:854	intestinal lactase	837:854	intestinal lactase between patients with and without lactose intolerance	837:908	We compared intestinal lactase between patients with and without lactose intolerance.					
36868181	7	0	theme	enhanced	1527:1534	arg1	adhesion					1536:1543	adhesion	1536:1543	adhesion	1536:1543	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	4	1	theme	Ti	927:928	arg1	Ti-Se5					950:955	Ti-Se5	950:955	Ti-Se5	950:955	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	1	theme	Ti	927:928	arg1	Ti-Se10					962:968	Ti-Se10	962:968	Ti-Se10	962:968	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	1	theme	Ti	927:928	arg1	Ti-Se1					942:947	Ti-Se1	942:947	Ti-Se1	942:947	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	1	theme	Ti	927:928	arg1	substrates					930:939	SLA Ti substrates	923:939	SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10)	923:969	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	8	2	theme	nuclear	1743:1749	arg1	B					1764:1764	nuclear factor kappa B	1743:1764	the nuclear factor kappa B pathway in Raw 264.7 cells	1739:1791	In addition, Ti-Se1, Ti-Se5, and Ti-Se10 surfaces modulated the secretion of pro-/anti-inflammatory cytokines by inhibiting the nuclear factor kappa B pathway in Raw 264.7 cells.					
36868181	5	3	theme	Ti	1212:1213	arg1	pretreatment					1225:1236	Ti substrate pretreatment	1212:1236	Ti substrate pretreatment	1212:1236	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	7	4	theme	good	1466:1469	arg1	biocompatibility					1471:1486	good biocompatibility	1466:1486	good biocompatibility	1466:1486	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	6	5	theme	spectroscopy	1295:1306	arg1	analysis					1308:1315	X-ray photoelectron spectroscopy analysis	1275:1315	X-ray photoelectron spectroscopy analysis	1275:1315	Besides, X-ray photoelectron spectroscopy analysis showed that CS-SeNPs were successfully anchored to Ti surfaces.					
36868181	8	6	theme	kappa	1758:1762	arg1	B					1764:1764	nuclear factor kappa B	1743:1764	the nuclear factor kappa B pathway in Raw 264.7 cells	1739:1791	In addition, Ti-Se1, Ti-Se5, and Ti-Se10 surfaces modulated the secretion of pro-/anti-inflammatory cytokines by inhibiting the nuclear factor kappa B pathway in Raw 264.7 cells.					
36868181	2	7	theme	anti-inflammatory	660:676	arg1	activities					678:687	osteogenic and anti-inflammatory activities	645:687	its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro	606:696	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	9	8	theme	osteogenic	1919:1928	arg1	activities					1952:1961	the osteogenic and anti-inflammatory activities	1915:1961	the osteogenic and anti-inflammatory activities of Ti implants	1915:1976	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	6	9	theme	X-ray	1275:1279	arg1	analysis					1308:1315	X-ray photoelectron spectroscopy analysis	1275:1315	X-ray photoelectron spectroscopy analysis	1275:1315	Besides, X-ray photoelectron spectroscopy analysis showed that CS-SeNPs were successfully anchored to Ti surfaces.					
36868181	9	10	theme	doping	1809:1814	arg1	strategy					1895:1902	a promising strategy	1883:1902	a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants	1883:1976	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	9	10	theme	doping	1809:1814	arg1	substrates					1823:1832	doping SLA Ti substrates	1809:1832	doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM)	1809:1874	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	2	11	theme	osteogenic	645:654	arg1	activities					678:687	osteogenic and anti-inflammatory activities	645:687	its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro	606:696	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	7	12	theme	as-prepared	1432:1442	arg1	surfaces					1447:1454	the four as-prepared Ti surfaces	1423:1454	the four as-prepared Ti surfaces	1423:1454	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	9	13	theme	anti-inflammatory	1934:1950	arg1	activities					1952:1961	the osteogenic and anti-inflammatory activities	1915:1961	the osteogenic and anti-inflammatory activities of Ti implants	1915:1976	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	8	14	theme	Raw	1777:1779	arg1	cells					1787:1791	Raw 264.7 cells	1777:1791	Raw 264.7 cells	1777:1791	In addition, Ti-Se1, Ti-Se5, and Ti-Se10 surfaces modulated the secretion of pro-/anti-inflammatory cytokines by inhibiting the nuclear factor kappa B pathway in Raw 264.7 cells.					
36868181	1	15	theme	dental	211:216	arg1	implants					218:225	dental implants	211:225	dental implants	211:225	Osseointegration is a prerequisite for the function of dental implants, and macrophage-dominated immune responses triggered by implantation determine the outcome of ultimate bone healing mediated by osteogenic cells.					
36868181	3	16	theme	elemental	793:801	arg1	composition					803:813	elemental composition	793:813	elemental composition	793:813	CS-SeNPs were successfully prepared by chemical synthesis and characterized their morphology, elemental composition, particle size, and Zeta potential.					
36868181	0	17	theme	coupling	137:144	arg1	strategy					146:153	a covalent coupling strategy	126:153	a covalent coupling strategy	126:153	Osteogenic and anti-inflammatory effects of SLA titanium substrates doped with chitosan-stabilized selenium nanoparticles via a covalent coupling strategy.					
36868181	5	18	theme	electron	1071:1078	arg1	microscopy					1080:1089	Scanning electron microscopy	1062:1089	Scanning electron microscopy images	1062:1096	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	9	19	theme	implants	1969:1976	arg1	activities					1952:1961	the osteogenic and anti-inflammatory activities	1915:1961	the osteogenic and anti-inflammatory activities of Ti implants	1915:1976	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	9	20	theme	modest	1841:1846	arg1	CS-SeNPs					1858:1865	CS-SeNPs	1858:1865	CS-SeNPs (1-5 mM)	1858:1874	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	9	20	theme	modest	1841:1846	arg1	amount					1848:1853	a modest amount	1839:1853	a modest amount of CS-SeNPs (1-5 mM)	1839:1874	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	9	20	theme	modest	1841:1846	arg1	1-5 mM					1868:1873	1-5 mM	1868:1873	1-5 mM	1868:1873	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	7	21	dep	in	1396:1397	arg1	vitro					1399:1403	vitro	1399:1403	vitro	1399:1403	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	2	22	theme	grit	544:547	arg1	substrates					575:584	sandblasted, large grit, and acid-etched (SLA) Ti substrates	525:584	sandblasted, large grit, and acid-etched (SLA) Ti substrates	525:584	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	4	23	used	used	1043:1046	arg2	Ti-SLA					1031:1036	the SLA Ti surface (Ti-SLA)	1011:1037	the SLA Ti surface (Ti-SLA)	1011:1037	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	23	used	used	1043:1046	arg2	control					1053:1059	a control	1051:1059	a control	1051:1059	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	24	theme	SLA	1015:1017	arg1	Ti-SLA					1031:1036	the SLA Ti surface (Ti-SLA)	1011:1037	the SLA Ti surface (Ti-SLA)	1011:1037	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	24	theme	SLA	1015:1017	arg1	control					1053:1059	a control	1051:1059	a control	1051:1059	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	5	25	dep	susceptible	1197:1207	arg1	immobilization					1250:1263	immobilization	1250:1263	immobilization	1250:1263	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	0	26	theme	chitosan-stabilized	79:97	arg1	nanoparticles					108:120	chitosan-stabilized selenium nanoparticles	79:120	chitosan-stabilized selenium nanoparticles	79:120	Osteogenic and anti-inflammatory effects of SLA titanium substrates doped with chitosan-stabilized selenium nanoparticles via a covalent coupling strategy.					
36868181	9	27	theme	CS-SeNPs	1858:1865	arg1	CS-SeNPs					1858:1865	CS-SeNPs	1858:1865	CS-SeNPs (1-5 mM)	1858:1874	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	9	27	theme	CS-SeNPs	1858:1865	arg1	amount					1848:1853	a modest amount	1839:1853	a modest amount of CS-SeNPs (1-5 mM)	1839:1874	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	9	27	theme	CS-SeNPs	1858:1865	arg1	1-5 mM					1868:1873	1-5 mM	1868:1873	1-5 mM	1868:1873	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	4	28	theme	covalent	979:986	arg1	strategy					997:1004	a covalent coupling strategy	977:1004	a covalent coupling strategy	977:1004	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	5	29	theme	different	1107:1115	arg1	CS-SeNPs					1128:1135	CS-SeNPs	1128:1135	CS-SeNPs	1128:1135	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	5	29	theme	different	1107:1115	arg1	amounts					1117:1123	different amounts	1107:1123	different amounts of CS-SeNPs	1107:1135	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	7	30	theme	Ti-SLA	1601:1606	arg1	group					1608:1612	the Ti-SLA group	1597:1612	the Ti-SLA group	1597:1612	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	9	31	theme	Ti	1820:1821	arg1	strategy					1895:1902	a promising strategy	1883:1902	a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants	1883:1976	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	9	31	theme	Ti	1820:1821	arg1	substrates					1823:1832	doping SLA Ti substrates	1809:1832	doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM)	1809:1874	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	2	32	theme	titanium	419:426	arg1	surface					433:439	a modified titanium (Ti) surface	408:439	a modified titanium (Ti) surface	408:439	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	0	33	theme	Osteogenic	0:9	arg1	effects					33:39	Osteogenic and anti-inflammatory effects	0:39	Osteogenic and anti-inflammatory effects of SLA titanium substrates	0:66	Osteogenic and anti-inflammatory effects of SLA titanium substrates doped with chitosan-stabilized selenium nanoparticles via a covalent coupling strategy.					
36868181	5	34	theme	surfaces	1178:1185	arg1	wettability					1160:1170	wettability	1160:1170	wettability	1160:1170	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	5	34	theme	surfaces	1178:1185	arg1	roughness					1146:1154	roughness	1146:1154	roughness	1146:1154	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	2	35	theme	chitosan-stabilized	468:486	arg1	CS-SeNPs					512:519	CS-SeNPs	512:519	CS-SeNPs	512:519	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	2	35	theme	chitosan-stabilized	468:486	arg1	nanoparticles					497:509	chitosan-stabilized selenium nanoparticles	468:509	chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates	468:584	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	5	36	dep	roughness	1146:1154	arg1	the					1142:1144	the	1142:1144	the	1142:1144	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	8	37	from	pathway	1766:1772	arg1	cells					1787:1791	Raw 264.7 cells	1777:1791	Raw 264.7 cells	1777:1791	In addition, Ti-Se1, Ti-Se5, and Ti-Se10 surfaces modulated the secretion of pro-/anti-inflammatory cytokines by inhibiting the nuclear factor kappa B pathway in Raw 264.7 cells.					
36868181	0	38	theme	anti-inflammatory	15:31	arg1	effects					33:39	Osteogenic and anti-inflammatory effects	0:39	Osteogenic and anti-inflammatory effects of SLA titanium substrates	0:66	Osteogenic and anti-inflammatory effects of SLA titanium substrates doped with chitosan-stabilized selenium nanoparticles via a covalent coupling strategy.					
36868181	1	39	theme	bone	330:333	arg1	healing					335:341	ultimate bone healing	321:341	ultimate bone healing mediated by osteogenic cells	321:370	Osseointegration is a prerequisite for the function of dental implants, and macrophage-dominated immune responses triggered by implantation determine the outcome of ultimate bone healing mediated by osteogenic cells.					
36868181	4	40	theme	surface	1022:1028	arg1	Ti-SLA					1031:1036	the SLA Ti surface (Ti-SLA)	1011:1037	the SLA Ti surface (Ti-SLA)	1011:1037	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	40	theme	surface	1022:1028	arg1	control					1053:1059	a control	1051:1059	a control	1051:1059	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	41	theme	different	871:879	arg1	concentrations					881:894	three different concentrations	865:894	three different concentrations of CS-SeNPs	865:906	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	42	dep	substrates	930:939	arg1	Ti-Se5					950:955	Ti-Se5	950:955	Ti-Se5	950:955	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	42	dep	substrates	930:939	arg1	Ti-Se10					962:968	Ti-Se10	962:968	Ti-Se10	962:968	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	42	dep	substrates	930:939	arg1	Ti-Se1					942:947	Ti-Se1	942:947	Ti-Se1	942:947	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	42	dep	substrates	930:939	arg1	substrates					930:939	SLA Ti substrates	923:939	SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10)	923:969	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	2	43	dep	acid-etched	554:564	arg1	SLA					567:569	SLA	567:569	SLA	567:569	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	7	44	theme	MC3T3-E1	1568:1575	arg1	cells					1577:1581	MC3T3-E1 cells	1568:1581	MC3T3-E1 cells	1568:1581	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	0	45	theme	titanium	48:55	arg1	substrates					57:66	SLA titanium substrates	44:66	SLA titanium substrates	44:66	Osteogenic and anti-inflammatory effects of SLA titanium substrates doped with chitosan-stabilized selenium nanoparticles via a covalent coupling strategy.					
36868181	1	46	theme	osteogenic	355:364	arg1	cells					366:370	osteogenic cells	355:370	osteogenic cells	355:370	Osseointegration is a prerequisite for the function of dental implants, and macrophage-dominated immune responses triggered by implantation determine the outcome of ultimate bone healing mediated by osteogenic cells.					
36868181	1	47	theme	immune	253:258	arg1	responses					260:268	macrophage-dominated immune responses	232:268	macrophage-dominated immune responses triggered by implantation	232:294	Osseointegration is a prerequisite for the function of dental implants, and macrophage-dominated immune responses triggered by implantation determine the outcome of ultimate bone healing mediated by osteogenic cells.					
36868181	8	48	theme	pro-/anti-inflammatory	1692:1713	arg1	cytokines					1715:1723	pro-/anti-inflammatory cytokines	1692:1723	pro-/anti-inflammatory cytokines	1692:1723	In addition, Ti-Se1, Ti-Se5, and Ti-Se10 surfaces modulated the secretion of pro-/anti-inflammatory cytokines by inhibiting the nuclear factor kappa B pathway in Raw 264.7 cells.					
36868181	9	49	theme	promising	1885:1893	arg1	strategy					1895:1902	a promising strategy	1883:1902	a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants	1883:1976	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	9	49	theme	promising	1885:1893	arg1	substrates					1823:1832	doping SLA Ti substrates	1809:1832	doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM)	1809:1874	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	7	50	theme	Ti-Se5	1505:1510	arg1	groups					1512:1517	Ti-Se1 and Ti-Se5 groups	1494:1517	groups	1512:1517	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	4	51	theme	SLA	923:925	arg1	Ti-Se5					950:955	Ti-Se5	950:955	Ti-Se5	950:955	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	51	theme	SLA	923:925	arg1	Ti-Se10					962:968	Ti-Se10	962:968	Ti-Se10	962:968	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	51	theme	SLA	923:925	arg1	Ti-Se1					942:947	Ti-Se1	942:947	Ti-Se1	942:947	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	51	theme	SLA	923:925	arg1	substrates					930:939	SLA Ti substrates	923:939	SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10)	923:969	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	8	52	theme	Ti-Se10	1648:1654	arg1	surfaces					1656:1663	Ti-Se10 surfaces	1648:1663	Ti-Se10 surfaces	1648:1663	In addition, Ti-Se1, Ti-Se5, and Ti-Se10 surfaces modulated the secretion of pro-/anti-inflammatory cytokines by inhibiting the nuclear factor kappa B pathway in Raw 264.7 cells.					
36868181	3	53	theme	chemical	738:745	arg1	synthesis					747:755	chemical synthesis	738:755	chemical synthesis	738:755	CS-SeNPs were successfully prepared by chemical synthesis and characterized their morphology, elemental composition, particle size, and Zeta potential.					
36868181	5	54	theme	substrate	1215:1223	arg1	pretreatment					1225:1236	Ti substrate pretreatment	1212:1236	Ti substrate pretreatment	1212:1236	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	7	55	theme	Ti-Se1	1494:1499	arg1	groups					1512:1517	Ti-Se1 and Ti-Se5 groups	1494:1517	groups	1512:1517	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	2	56	theme	surface	610:616	arg1	characteristics					618:632	its surface characteristics	606:632	its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro	606:696	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	6	57	theme	photoelectron	1281:1293	arg1	analysis					1308:1315	X-ray photoelectron spectroscopy analysis	1275:1315	X-ray photoelectron spectroscopy analysis	1275:1315	Besides, X-ray photoelectron spectroscopy analysis showed that CS-SeNPs were successfully anchored to Ti surfaces.					
36868181	8	58	theme	factor	1751:1756	arg1	B					1764:1764	nuclear factor kappa B	1743:1764	the nuclear factor kappa B pathway in Raw 264.7 cells	1739:1791	In addition, Ti-Se1, Ti-Se5, and Ti-Se10 surfaces modulated the secretion of pro-/anti-inflammatory cytokines by inhibiting the nuclear factor kappa B pathway in Raw 264.7 cells.					
36868181	4	59	theme	CS-SeNPs	899:906	arg1	concentrations					881:894	three different concentrations	865:894	three different concentrations of CS-SeNPs	865:906	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	9	60	from	strategy	1895:1902	arg1	conclusion					1797:1806	conclusion	1797:1806	conclusion	1797:1806	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	8	61	theme	B	1764:1764	arg1	pathway					1766:1772	the nuclear factor kappa B pathway	1739:1772	the nuclear factor kappa B pathway in Raw 264.7 cells	1739:1791	In addition, Ti-Se1, Ti-Se5, and Ti-Se10 surfaces modulated the secretion of pro-/anti-inflammatory cytokines by inhibiting the nuclear factor kappa B pathway in Raw 264.7 cells.					
36868181	7	62	theme	Ti	1444:1445	arg1	surfaces					1447:1454	the four as-prepared Ti surfaces	1423:1454	the four as-prepared Ti surfaces	1423:1454	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	3	63	theme	particle	816:823	arg1	size					825:828	particle size	816:828	particle size	816:828	CS-SeNPs were successfully prepared by chemical synthesis and characterized their morphology, elemental composition, particle size, and Zeta potential.					
36868181	1	64	theme	implants	218:225	arg1	function					199:206	the function	195:206	the function of dental implants	195:225	Osseointegration is a prerequisite for the function of dental implants, and macrophage-dominated immune responses triggered by implantation determine the outcome of ultimate bone healing mediated by osteogenic cells.					
36868181	2	65	theme	Ti	572:573	arg1	substrates					575:584	sandblasted, large grit, and acid-etched (SLA) Ti substrates	525:584	sandblasted, large grit, and acid-etched (SLA) Ti substrates	525:584	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	9	66	theme	Ti	1966:1967	arg1	implants					1969:1976	Ti implants	1966:1976	Ti implants	1966:1976	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	6	67	theme	Ti	1368:1369	arg1	surfaces					1371:1378	Ti surfaces	1368:1378	Ti surfaces	1368:1378	Besides, X-ray photoelectron spectroscopy analysis showed that CS-SeNPs were successfully anchored to Ti surfaces.					
36868181	5	68	theme	microscopy	1080:1089	arg1	images					1091:1096	Scanning electron microscopy images	1062:1096	Scanning electron microscopy images	1062:1096	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	3	69	theme	Zeta	835:838	arg1	potential					840:848	Zeta potential	835:848	Zeta potential	835:848	CS-SeNPs were successfully prepared by chemical synthesis and characterized their morphology, elemental composition, particle size, and Zeta potential.					
36868181	7	70	theme	study	1405:1409	arg1	results					1385:1391	The results	1381:1391	The results of in vitro study	1381:1409	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	2	71	from	activities	678:687	arg1	vitro					692:696	vitro	692:696	vitro	692:696	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	7	72	theme	in	1396:1397	arg1	study					1405:1409	in vitro study	1396:1409	in vitro study	1396:1409	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	5	73	theme	Scanning	1062:1069	arg1	microscopy					1080:1089	Scanning electron microscopy	1062:1089	Scanning electron microscopy images	1062:1096	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	2	74	theme	large	538:542	arg1	grit					544:547	large grit	538:547	large grit	538:547	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	9	75	theme	SLA	1816:1818	arg1	strategy					1895:1902	a promising strategy	1883:1902	a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants	1883:1976	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	9	75	theme	SLA	1816:1818	arg1	substrates					1823:1832	doping SLA Ti substrates	1809:1832	doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM)	1809:1874	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	0	76	theme	selenium	99:106	arg1	nanoparticles					108:120	chitosan-stabilized selenium nanoparticles	79:120	chitosan-stabilized selenium nanoparticles	79:120	Osteogenic and anti-inflammatory effects of SLA titanium substrates doped with chitosan-stabilized selenium nanoparticles via a covalent coupling strategy.					
36868181	2	77	theme	sandblasted	525:535	arg1	substrates					575:584	sandblasted, large grit, and acid-etched (SLA) Ti substrates	525:584	sandblasted, large grit, and acid-etched (SLA) Ti substrates	525:584	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	2	78	theme	acid-etched	554:564	arg1	substrates					575:584	sandblasted, large grit, and acid-etched (SLA) Ti substrates	525:584	sandblasted, large grit, and acid-etched (SLA) Ti substrates	525:584	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	0	79	theme	covalent	128:135	arg1	strategy					146:153	a covalent coupling strategy	126:153	a covalent coupling strategy	126:153	Osteogenic and anti-inflammatory effects of SLA titanium substrates doped with chitosan-stabilized selenium nanoparticles via a covalent coupling strategy.					
36868181	4	80	theme	coupling	988:995	arg1	strategy					997:1004	a covalent coupling strategy	977:1004	a covalent coupling strategy	977:1004	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	5	81	theme	CS-SeNPs	1128:1135	arg1	CS-SeNPs					1128:1135	CS-SeNPs	1128:1135	CS-SeNPs	1128:1135	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	5	81	theme	CS-SeNPs	1128:1135	arg1	amounts					1117:1123	different amounts	1107:1123	different amounts of CS-SeNPs	1107:1135	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	2	82	theme	selenium	488:495	arg1	CS-SeNPs					512:519	CS-SeNPs	512:519	CS-SeNPs	512:519	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	2	82	theme	selenium	488:495	arg1	nanoparticles					497:509	chitosan-stabilized selenium nanoparticles	468:509	chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates	468:584	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	2	83	from	characteristics	618:632	arg1	vitro					692:696	vitro	692:696	vitro	692:696	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	2	84	theme	modified	410:417	arg1	surface					433:439	a modified titanium (Ti) surface	408:439	a modified titanium (Ti) surface	408:439	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	1	85	theme	ultimate	321:328	arg1	healing					335:341	ultimate bone healing	321:341	ultimate bone healing mediated by osteogenic cells	321:370	Osseointegration is a prerequisite for the function of dental implants, and macrophage-dominated immune responses triggered by implantation determine the outcome of ultimate bone healing mediated by osteogenic cells.					
36868181	0	86	theme	SLA	44:46	arg1	substrates					57:66	SLA titanium substrates	44:66	SLA titanium substrates	44:66	Osteogenic and anti-inflammatory effects of SLA titanium substrates doped with chitosan-stabilized selenium nanoparticles via a covalent coupling strategy.					
36868181	1	87	theme	healing	335:341	arg1	outcome					310:316	the outcome	306:316	the outcome of ultimate bone healing mediated by osteogenic cells	306:370	Osseointegration is a prerequisite for the function of dental implants, and macrophage-dominated immune responses triggered by implantation determine the outcome of ultimate bone healing mediated by osteogenic cells.					
36868181	4	88	theme	Ti	1019:1020	arg1	Ti-SLA					1031:1036	the SLA Ti surface (Ti-SLA)	1011:1037	the SLA Ti surface (Ti-SLA)	1011:1037	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	4	88	theme	Ti	1019:1020	arg1	control					1053:1059	a control	1051:1059	a control	1051:1059	Subsequently, three different concentrations of CS-SeNPs were loaded to SLA Ti substrates (Ti-Se1, Ti-Se5, and Ti-Se10) using a covalent coupling strategy, and the SLA Ti surface (Ti-SLA) was used as a control.					
36868181	0	89	theme	substrates	57:66	arg1	effects					33:39	Osteogenic and anti-inflammatory effects	0:39	Osteogenic and anti-inflammatory effects of SLA titanium substrates	0:66	Osteogenic and anti-inflammatory effects of SLA titanium substrates doped with chitosan-stabilized selenium nanoparticles via a covalent coupling strategy.					
36868181	7	90	theme	cells	1577:1581	arg1	differentiation					1549:1563	differentiation	1549:1563	differentiation	1549:1563	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	7	90	theme	cells	1577:1581	arg1	adhesion					1536:1543	adhesion	1536:1543	adhesion	1536:1543	The results of in vitro study showed that the four as-prepared Ti surfaces exhibited good biocompatibility, with Ti-Se1 and Ti-Se5 groups showing enhanced adhesion and differentiation of MC3T3-E1 cells compared with the Ti-SLA group.					
36868181	2	91	theme	present	377:383	arg1	study					385:389	The present study	373:389	The present study	373:389	The present study aimed to develop a modified titanium (Ti) surface by covalently immobilizing chitosan-stabilized selenium nanoparticles (CS-SeNPs) to sandblasted, large grit, and acid-etched (SLA) Ti substrates and further explore its surface characteristics as well as osteogenic and anti-inflammatory activities in vitro.					
36868181	5	92	theme	Ti	1175:1176	arg1	surfaces					1178:1185	Ti surfaces	1175:1185	Ti surfaces	1175:1185	Scanning electron microscopy images revealed different amounts of CS-SeNPs, and the roughness and wettability of Ti surfaces were less susceptible to Ti substrate pretreatment and CS-SeNP immobilization.					
36868181	9	93	with	substrates	1823:1832	arg1	CS-SeNPs					1858:1865	CS-SeNPs	1858:1865	CS-SeNPs (1-5 mM)	1858:1874	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	9	93	with	substrates	1823:1832	arg1	amount					1848:1853	a modest amount	1839:1853	a modest amount of CS-SeNPs (1-5 mM)	1839:1874	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	9	93	with	substrates	1823:1832	arg1	1-5 mM					1868:1873	1-5 mM	1868:1873	1-5 mM	1868:1873	In conclusion, doping SLA Ti substrates with a modest amount of CS-SeNPs (1-5 mM) may be a promising strategy to improve the osteogenic and anti-inflammatory activities of Ti implants.					
36868181	8	94	theme	cytokines	1715:1723	arg1	secretion					1679:1687	the secretion	1675:1687	the secretion of pro-/anti-inflammatory cytokines	1675:1723	In addition, Ti-Se1, Ti-Se5, and Ti-Se10 surfaces modulated the secretion of pro-/anti-inflammatory cytokines by inhibiting the nuclear factor kappa B pathway in Raw 264.7 cells.					
36868181	1	95	theme	macrophage-dominated	232:251	arg1	responses					260:268	macrophage-dominated immune responses	232:268	macrophage-dominated immune responses triggered by implantation	232:294	Osseointegration is a prerequisite for the function of dental implants, and macrophage-dominated immune responses triggered by implantation determine the outcome of ultimate bone healing mediated by osteogenic cells.					
36325648	6	0	theme	cell	788:791	arg1	transplantation					793:807	islet cell transplantation	782:807	islet cell transplantation	782:807	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	1	1	theme	native	175:180	arg1	function					191:198	native pancreas function	175:198	native pancreas function	175:198	Self-regulated insulin delivery that mimics native pancreas function has been a long-term goal for diabetes therapies.					
36325648	4	2	theme	glycemic	571:578	arg1	control					580:586	glycemic control	571:586	glycemic control in diabetic mice	571:603	Material composition and formulation are tuned in each of these contexts to enable glycemic control in diabetic mice.					
36325648	6	3	theme	alginate	879:886	arg1	blend					841:845	a blend	839:845	a blend of unoxidized and 2.5% oxidized alginate	839:886	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	1	4	theme	pancreas	182:189	arg1	function					191:198	native pancreas function	175:198	native pancreas function	175:198	Self-regulated insulin delivery that mimics native pancreas function has been a long-term goal for diabetes therapies.					
36325648	0	5	theme	Cell	105:108	arg1	Therapy					122:128	Islet Cell Replacement Therapy	99:128	Islet Cell Replacement Therapy	99:128	Partially Oxidized Alginate as a Biodegradable Carrier for Glucose-Responsive Insulin Delivery and Islet Cell Replacement Therapy.					
36325648	6	6	theme	unoxidized	850:859	arg1	blend					841:845	a blend	839:845	a blend of unoxidized and 2.5% oxidized alginate	839:886	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	7	7	theme	limited	1167:1173	arg1	accumulation					1184:1195	limited material accumulation	1167:1195	limited material accumulation in the event of multiple doses	1167:1226	These data show the potential of these biodegradable carriers for controlled drug and cell delivery for the treatment of diabetes with limited material accumulation in the event of multiple doses.					
36325648	6	8	theme	oxidized	870:877	arg1	alginate					879:886	2.5% oxidized alginate	865:886	2.5% oxidized alginate	865:886	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	3	9	theme	alginate	403:410	arg1	carriers					412:419	biodegradable, partially oxidized alginate carriers	369:419	biodegradable, partially oxidized alginate carriers for glucose-responsive nanoparticles or islet cells	369:471	Here, biodegradable, partially oxidized alginate carriers for glucose-responsive nanoparticles or islet cells are developed.					
36325648	7	10	theme	material	1175:1182	arg1	accumulation					1184:1195	limited material accumulation	1167:1195	limited material accumulation in the event of multiple doses	1167:1226	These data show the potential of these biodegradable carriers for controlled drug and cell delivery for the treatment of diabetes with limited material accumulation in the event of multiple doses.					
36325648	6	11	theme	%	868:868	arg1	alginate					879:886	2.5% oxidized alginate	865:886	2.5% oxidized alginate	865:886	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	5	12	dep	injectable	610:619	arg1	glucose-responsive					622:639	glucose-responsive	622:639	glucose-responsive	622:639	For injectable, glucose-responsive insulin delivery, 0.5 mm 2.5% oxidized alginate microgels facilitate repeat dosing and consistently provide 10 days of glycemic control.					
36325648	6	13	theme	islet	782:786	arg1	transplantation					793:807	islet cell transplantation	782:807	islet cell transplantation	782:807	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	5	14	theme	alginate	680:687	arg1	microgels					689:697	0.5 mm 2.5% oxidized alginate microgels	659:697	0.5 mm 2.5% oxidized alginate microgels	659:697	For injectable, glucose-responsive insulin delivery, 0.5 mm 2.5% oxidized alginate microgels facilitate repeat dosing and consistently provide 10 days of glycemic control.					
36325648	5	15	theme	oxidized	671:678	arg1	microgels					689:697	0.5 mm 2.5% oxidized alginate microgels	659:697	0.5 mm 2.5% oxidized alginate microgels	659:697	For injectable, glucose-responsive insulin delivery, 0.5 mm 2.5% oxidized alginate microgels facilitate repeat dosing and consistently provide 10 days of glycemic control.					
36325648	2	16	theme	insulin	306:312	arg1	delivery					314:321	glucose-responsive insulin delivery	287:321	glucose-responsive insulin delivery	287:321	Two approaches towards this goal are glucose-responsive insulin delivery and islet cell transplantation therapy.					
36325648	2	16	theme	insulin	306:312	arg1	approaches					254:263	Two approaches	250:263	Two approaches towards this goal	250:281	Two approaches towards this goal are glucose-responsive insulin delivery and islet cell transplantation therapy.					
36325648	7	17	theme	diabetes	1153:1160	arg1	treatment					1140:1148	the treatment	1136:1148	the treatment of diabetes with limited material accumulation in the event of multiple doses	1136:1226	These data show the potential of these biodegradable carriers for controlled drug and cell delivery for the treatment of diabetes with limited material accumulation in the event of multiple doses.					
36325648	0	18	theme	Replacement	110:120	arg1	Therapy					122:128	Islet Cell Replacement Therapy	99:128	Islet Cell Replacement Therapy	99:128	Partially Oxidized Alginate as a Biodegradable Carrier for Glucose-Responsive Insulin Delivery and Islet Cell Replacement Therapy.					
36325648	6	19	theme	glycemic	916:923	arg1	control					925:931	glycemic control	916:931	glycemic control	916:931	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	2	20	theme	glucose-responsive	287:304	arg1	delivery					314:321	glucose-responsive insulin delivery	287:321	glucose-responsive insulin delivery	287:321	Two approaches towards this goal are glucose-responsive insulin delivery and islet cell transplantation therapy.					
36325648	2	20	theme	glucose-responsive	287:304	arg1	approaches					254:263	Two approaches	250:263	Two approaches towards this goal	250:281	Two approaches towards this goal are glucose-responsive insulin delivery and islet cell transplantation therapy.					
36325648	3	21	theme	glucose-responsive	425:442	arg1	nanoparticles					444:456	glucose-responsive nanoparticles	425:456	glucose-responsive nanoparticles	425:456	Here, biodegradable, partially oxidized alginate carriers for glucose-responsive nanoparticles or islet cells are developed.					
36325648	5	22	theme	insulin	641:647	arg1	delivery					649:656	injectable, glucose-responsive insulin delivery	610:656	injectable, glucose-responsive insulin delivery	610:656	For injectable, glucose-responsive insulin delivery, 0.5 mm 2.5% oxidized alginate microgels facilitate repeat dosing and consistently provide 10 days of glycemic control.					
36325648	6	23	theme	months	962:967	arg1	period					940:945	a period	938:945	a period of more than 2 months	938:967	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	5	24	theme	repeat	710:715	arg1	dosing					717:722	repeat dosing	710:722	repeat dosing	710:722	For injectable, glucose-responsive insulin delivery, 0.5 mm 2.5% oxidized alginate microgels facilitate repeat dosing and consistently provide 10 days of glycemic control.					
36325648	2	25	theme	transplantation	338:352	arg1	therapy					354:360	islet cell transplantation therapy	327:360	islet cell transplantation therapy	327:360	Two approaches towards this goal are glucose-responsive insulin delivery and islet cell transplantation therapy.					
36325648	7	26	theme	doses	1222:1226	arg1	event					1204:1208	the event	1200:1208	the event of multiple doses	1200:1226	These data show the potential of these biodegradable carriers for controlled drug and cell delivery for the treatment of diabetes with limited material accumulation in the event of multiple doses.					
36325648	5	27	theme	control	769:775	arg1	days					752:755	10 days	749:755	10 days of glycemic control	749:775	For injectable, glucose-responsive insulin delivery, 0.5 mm 2.5% oxidized alginate microgels facilitate repeat dosing and consistently provide 10 days of glycemic control.					
36325648	6	28	theme	2.5	865:867	arg1	%					868:868	%	868:868	%	868:868	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	2	29	theme	cell	333:336	arg1	therapy					354:360	islet cell transplantation therapy	327:360	islet cell transplantation therapy	327:360	Two approaches towards this goal are glucose-responsive insulin delivery and islet cell transplantation therapy.					
36325648	1	30	theme	long-term	211:219	arg1	goal					221:224	a long-term goal	209:224	a long-term goal for diabetes therapies	209:247	Self-regulated insulin delivery that mimics native pancreas function has been a long-term goal for diabetes therapies.					
36325648	1	30	theme	long-term	211:219	arg1	delivery					154:161	Self-regulated insulin delivery	131:161	Self-regulated insulin delivery that mimics native pancreas function	131:198	Self-regulated insulin delivery that mimics native pancreas function has been a long-term goal for diabetes therapies.					
36325648	2	31	theme	islet	327:331	arg1	therapy					354:360	islet cell transplantation therapy	327:360	islet cell transplantation therapy	327:360	Two approaches towards this goal are glucose-responsive insulin delivery and islet cell transplantation therapy.					
36325648	0	32	theme	Biodegradable	33:45	arg1	Alginate					19:26	Alginate	19:26	Alginate	19:26	Partially Oxidized Alginate as a Biodegradable Carrier for Glucose-Responsive Insulin Delivery and Islet Cell Replacement Therapy.					
36325648	0	32	theme	Biodegradable	33:45	arg1	Carrier					47:53	a Biodegradable Carrier	31:53	a Biodegradable Carrier for Glucose-Responsive Insulin Delivery and Islet Cell Replacement Therapy	31:128	Partially Oxidized Alginate as a Biodegradable Carrier for Glucose-Responsive Insulin Delivery and Islet Cell Replacement Therapy.					
36325648	6	33	theme	material	1012:1019	arg1	volume					988:993	the volume	984:993	the volume of nondegradable material implanted	984:1029	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	7	34	theme	cell	1118:1121	arg1	delivery					1123:1130	controlled drug and cell delivery	1098:1130	delivery	1123:1130	These data show the potential of these biodegradable carriers for controlled drug and cell delivery for the treatment of diabetes with limited material accumulation in the event of multiple doses.					
36325648	7	35	theme	multiple	1213:1220	arg1	doses					1222:1226	multiple doses	1213:1226	multiple doses	1213:1226	These data show the potential of these biodegradable carriers for controlled drug and cell delivery for the treatment of diabetes with limited material accumulation in the event of multiple doses.					
36325648	6	36	theme	nondegradable	998:1010	arg1	material					1012:1019	nondegradable material	998:1019	nondegradable material implanted	998:1029	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	3	37	dep	biodegradable	369:381	arg1	oxidized					394:401	oxidized	394:401	oxidized	394:401	Here, biodegradable, partially oxidized alginate carriers for glucose-responsive nanoparticles or islet cells are developed.					
36325648	7	38	theme	controlled	1098:1107	arg1	drug					1109:1112	controlled drug and cell delivery	1098:1130	drug	1109:1112	These data show the potential of these biodegradable carriers for controlled drug and cell delivery for the treatment of diabetes with limited material accumulation in the event of multiple doses.					
36325648	7	39	theme	biodegradable	1071:1083	arg1	carriers					1085:1092	these biodegradable carriers	1065:1092	these biodegradable carriers	1065:1092	These data show the potential of these biodegradable carriers for controlled drug and cell delivery for the treatment of diabetes with limited material accumulation in the event of multiple doses.					
36325648	1	40	theme	diabetes	230:237	arg1	therapies					239:247	diabetes therapies	230:247	diabetes therapies	230:247	Self-regulated insulin delivery that mimics native pancreas function has been a long-term goal for diabetes therapies.					
36325648	4	41	from	control	580:586	arg1	mice					600:603	diabetic mice	591:603	diabetic mice	591:603	Material composition and formulation are tuned in each of these contexts to enable glycemic control in diabetic mice.					
36325648	0	42	theme	Glucose-Responsive	59:76	arg1	Delivery					86:93	Glucose-Responsive Insulin Delivery	59:93	Glucose-Responsive Insulin Delivery	59:93	Partially Oxidized Alginate as a Biodegradable Carrier for Glucose-Responsive Insulin Delivery and Islet Cell Replacement Therapy.					
36325648	7	43	theme	carriers	1085:1092	arg1	potential					1052:1060	the potential	1048:1060	the potential of these biodegradable carriers for controlled drug and cell delivery	1048:1130	These data show the potential of these biodegradable carriers for controlled drug and cell delivery for the treatment of diabetes with limited material accumulation in the event of multiple doses.					
36325648	7	44	with	treatment	1140:1148	arg1	accumulation					1184:1195	limited material accumulation	1167:1195	limited material accumulation in the event of multiple doses	1167:1226	These data show the potential of these biodegradable carriers for controlled drug and cell delivery for the treatment of diabetes with limited material accumulation in the event of multiple doses.					
36325648	3	45	theme	islet	461:465	arg1	cells					467:471	islet cells	461:471	islet cells	461:471	Here, biodegradable, partially oxidized alginate carriers for glucose-responsive nanoparticles or islet cells are developed.					
36325648	7	46	from	accumulation	1184:1195	arg1	event					1204:1208	the event	1200:1208	the event of multiple doses	1200:1226	These data show the potential of these biodegradable carriers for controlled drug and cell delivery for the treatment of diabetes with limited material accumulation in the event of multiple doses.					
36325648	5	47	theme	injectable	610:619	arg1	delivery					649:656	injectable, glucose-responsive insulin delivery	610:656	injectable, glucose-responsive insulin delivery	610:656	For injectable, glucose-responsive insulin delivery, 0.5 mm 2.5% oxidized alginate microgels facilitate repeat dosing and consistently provide 10 days of glycemic control.					
36325648	1	48	theme	Self-regulated	131:144	arg1	goal					221:224	a long-term goal	209:224	a long-term goal for diabetes therapies	209:247	Self-regulated insulin delivery that mimics native pancreas function has been a long-term goal for diabetes therapies.					
36325648	1	48	theme	Self-regulated	131:144	arg1	delivery					154:161	Self-regulated insulin delivery	131:161	Self-regulated insulin delivery that mimics native pancreas function	131:198	Self-regulated insulin delivery that mimics native pancreas function has been a long-term goal for diabetes therapies.					
36325648	4	49	theme	diabetic	591:598	arg1	mice					600:603	diabetic mice	591:603	diabetic mice	591:603	Material composition and formulation are tuned in each of these contexts to enable glycemic control in diabetic mice.					
36325648	1	50	theme	insulin	146:152	arg1	goal					221:224	a long-term goal	209:224	a long-term goal for diabetes therapies	209:247	Self-regulated insulin delivery that mimics native pancreas function has been a long-term goal for diabetes therapies.					
36325648	1	50	theme	insulin	146:152	arg1	delivery					154:161	Self-regulated insulin delivery	131:161	Self-regulated insulin delivery that mimics native pancreas function	131:198	Self-regulated insulin delivery that mimics native pancreas function has been a long-term goal for diabetes therapies.					
36325648	5	51	dep	oxidized	671:678	arg1	%					669:669	2.5%	666:669	2.5%	666:669	For injectable, glucose-responsive insulin delivery, 0.5 mm 2.5% oxidized alginate microgels facilitate repeat dosing and consistently provide 10 days of glycemic control.					
36325648	5	52	theme	glycemic	760:767	arg1	control					769:775	glycemic control	760:775	glycemic control	760:775	For injectable, glucose-responsive insulin delivery, 0.5 mm 2.5% oxidized alginate microgels facilitate repeat dosing and consistently provide 10 days of glycemic control.					
36325648	6	53	theme	cell	897:900	arg1	viability					902:910	cell viability	897:910	cell viability	897:910	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	6	54	theme	1.5 mm	810:815	arg1	capsules					817:824	1.5 mm capsules	810:824	1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate	810:886	For islet cell transplantation, 1.5 mm capsules comprised of a blend of unoxidized and 2.5% oxidized alginate maintain cell viability and glycemic control over a period of more than 2 months while reducing the volume of nondegradable material implanted.					
36325648	0	55	theme	Insulin	78:84	arg1	Delivery					86:93	Glucose-Responsive Insulin Delivery	59:93	Glucose-Responsive Insulin Delivery	59:93	Partially Oxidized Alginate as a Biodegradable Carrier for Glucose-Responsive Insulin Delivery and Islet Cell Replacement Therapy.					
36325648	4	56	theme	Material	488:495	arg1	composition					497:507	Material composition	488:507	Material composition	488:507	Material composition and formulation are tuned in each of these contexts to enable glycemic control in diabetic mice.					
36325648	3	57	theme	biodegradable	369:381	arg1	carriers					412:419	biodegradable, partially oxidized alginate carriers	369:419	biodegradable, partially oxidized alginate carriers for glucose-responsive nanoparticles or islet cells	369:471	Here, biodegradable, partially oxidized alginate carriers for glucose-responsive nanoparticles or islet cells are developed.					
36325648	0	58	theme	Islet	99:103	arg1	Therapy					122:128	Islet Cell Replacement Therapy	99:128	Islet Cell Replacement Therapy	99:128	Partially Oxidized Alginate as a Biodegradable Carrier for Glucose-Responsive Insulin Delivery and Islet Cell Replacement Therapy.					
35436025	3	0	theme	factors	678:684	arg1	difficulty					718:727	the difficulty	714:727	the difficulty associated with modulating blood flow regimes	714:773	Interrogating the effects of shear stress in vivo is complicated by the complexity of factors in the brain parenchyma and the difficulty associated with modulating blood flow regimes.					
35436025	3	0	theme	factors	678:684	arg1	complexity					664:673	the complexity	660:673	the complexity of factors in the brain parenchyma	660:708	Interrogating the effects of shear stress in vivo is complicated by the complexity of factors in the brain parenchyma and the difficulty associated with modulating blood flow regimes.					
35436025	4	1	theme	real-time	833:841	arg1	measurement					843:853	real-time measurement	833:853	real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays	833:983	The in vitro model used in this study is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays.					
35436025	3	2	theme	brain	693:697	arg1	parenchyma					699:708	the brain parenchyma	689:708	the brain parenchyma	689:708	Interrogating the effects of shear stress in vivo is complicated by the complexity of factors in the brain parenchyma and the difficulty associated with modulating blood flow regimes.					
35436025	2	3	from	changes	535:541	arg1	perfusion					555:563	cerebral perfusion	546:563	cerebral perfusion including ischemic stroke	546:589	The goal of this study is to probe components of shear mechanotransduction within the BBB to gain a better understanding of pathologies associated with changes in cerebral perfusion including ischemic stroke.					
35436025	0	4	theme	barrier	74:80	arg1	model					82:86	an in vitro blood-brain barrier model	50:86	an in vitro blood-brain barrier model	50:86	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	5	5	theme	extracellular	1122:1134	arg1	matrix					1136:1141	the extracellular matrix	1118:1141	the extracellular matrix	1118:1141	These experiments reveal that there is a threshold level of shear stress required for barrier formation and that the composition of the extracellular matrix, specifically the presence of high molecular weight hyaluronan, dictates the flow response.					
35436025	2	6	theme	ischemic	575:582	arg1	stroke					584:589	ischemic stroke	575:589	ischemic stroke	575:589	The goal of this study is to probe components of shear mechanotransduction within the BBB to gain a better understanding of pathologies associated with changes in cerebral perfusion including ischemic stroke.					
35436025	0	7	theme	small	96:100	arg1	GTPases					102:108	small GTPases RhoA and Rac1	96:122	small GTPases RhoA and Rac1	96:122	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	0	7	theme	small	96:100	arg1	Rac1					119:122	Rac1	119:122	Rac1	119:122	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	0	7	theme	small	96:100	arg1	RhoA					110:113	RhoA	110:113	RhoA	110:113	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	5	8	theme	matrix	1136:1141	arg1	composition					1103:1113	the composition	1099:1113	the composition of the extracellular matrix	1099:1141	These experiments reveal that there is a threshold level of shear stress required for barrier formation and that the composition of the extracellular matrix, specifically the presence of high molecular weight hyaluronan, dictates the flow response.					
35436025	5	9	theme	high	1173:1176	arg1	weight					1188:1193	high molecular weight	1173:1193	high molecular weight hyaluronan	1173:1204	These experiments reveal that there is a threshold level of shear stress required for barrier formation and that the composition of the extracellular matrix, specifically the presence of high molecular weight hyaluronan, dictates the flow response.					
35436025	7	10	theme	small	1433:1437	arg1	activity					1446:1453	small GTPase activity	1433:1453	small GTPase activity	1433:1453	Manipulation of small GTPase activity reveals CD44 activates Rac1 while inhibiting RhoA activation.					
35436025	1	11	theme	shear	249:253	arg1	effect					239:244	the effect	235:244	the effect of shear on the blood-brain barrier (BBB)	235:286	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	1	12	theme	cerebral	316:323	arg1	vasculature					325:335	cerebral vasculature	316:335	cerebral vasculature	316:335	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	7	13	theme	GTPase	1439:1444	arg1	activity					1446:1453	small GTPase activity	1433:1453	small GTPase activity	1433:1453	Manipulation of small GTPase activity reveals CD44 activates Rac1 while inhibiting RhoA activation.					
35436025	0	14	dep	GTPases	102:108	arg1	GTPases					102:108	small GTPases RhoA and Rac1	96:122	small GTPases RhoA and Rac1	96:122	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	0	14	dep	GTPases	102:108	arg1	Rac1					119:122	Rac1	119:122	Rac1	119:122	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	0	14	dep	GTPases	102:108	arg1	RhoA					110:113	RhoA	110:113	RhoA	110:113	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	9	15	theme	specific	1681:1688	arg1	components					1690:1699	specific components	1681:1699	specific components of the mechanosensing complex associated with the BBB response to fluid shear stress	1681:1784	This study identifies specific components of the mechanosensing complex associated with the BBB response to fluid shear stress and, therefore, illuminates potential targets for barrier manipulation in vivo.					
35436025	9	16	theme	barrier	1836:1842	arg1	manipulation					1844:1855	barrier manipulation	1836:1855	barrier manipulation	1836:1855	This study identifies specific components of the mechanosensing complex associated with the BBB response to fluid shear stress and, therefore, illuminates potential targets for barrier manipulation in vivo.					
35436025	1	17	theme	important	150:158	arg1	stress					137:142	Fluid shear stress	125:142	Fluid shear stress	125:142	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	1	17	theme	important	150:158	arg1	mediator					160:167	an important mediator	147:167	an important mediator of vascular permeability	147:192	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	4	18	theme	immunocytochemistry	933:951	arg1	assays					978:983	immunocytochemistry and dextran permeability assays	933:983	a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays	881:983	The in vitro model used in this study is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays.					
35436025	2	19	theme	mechanotransduction	438:456	arg1	components					418:427	components	418:427	components of shear mechanotransduction	418:456	The goal of this study is to probe components of shear mechanotransduction within the BBB to gain a better understanding of pathologies associated with changes in cerebral perfusion including ischemic stroke.					
35436025	5	20	theme	hyaluronan	1195:1204	arg1	presence					1161:1168	the presence	1157:1168	the presence	1157:1168	These experiments reveal that there is a threshold level of shear stress required for barrier formation and that the composition of the extracellular matrix, specifically the presence of high molecular weight hyaluronan, dictates the flow response.					
35436025	8	21	theme	RhoA	1602:1605	arg1	inhibition					1607:1616	RhoA inhibition	1602:1616	RhoA inhibition	1602:1616	Additionally, adducin-γ localizes to tight junctions in response to shear stress and RhoA inhibition and is required to maintain the barrier.					
35436025	9	22	theme	potential	1814:1822	arg1	targets					1824:1830	potential targets	1814:1830	potential targets for barrier manipulation	1814:1855	This study identifies specific components of the mechanosensing complex associated with the BBB response to fluid shear stress and, therefore, illuminates potential targets for barrier manipulation in vivo.					
35436025	0	23	from	mechanotransduction	27:45	arg1	model					82:86	an in vitro blood-brain barrier model	50:86	an in vitro blood-brain barrier model	50:86	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	3	24	from	difficulty	718:727	arg1	parenchyma					699:708	the brain parenchyma	689:708	the brain parenchyma	689:708	Interrogating the effects of shear stress in vivo is complicated by the complexity of factors in the brain parenchyma and the difficulty associated with modulating blood flow regimes.					
35436025	4	25	theme	permeability	965:976	arg1	assays					978:983	immunocytochemistry and dextran permeability assays	933:983	a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays	881:983	The in vitro model used in this study is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays.					
35436025	9	26	theme	BBB	1751:1753	arg1	response					1755:1762	the BBB response	1747:1762	the BBB response to fluid shear stress	1747:1784	This study identifies specific components of the mechanosensing complex associated with the BBB response to fluid shear stress and, therefore, illuminates potential targets for barrier manipulation in vivo.					
35436025	6	27	theme	shear	1403:1407	arg1	stress					1409:1414	shear stress	1403:1414	shear stress	1403:1414	Gene editing to modulate the expression of CD44, a mechanosensitive receptor for hyaluronan, demonstrates that the receptor is required for the endothelial response to shear stress.					
35436025	9	28	dep	identifies	1670:1679	arg1	illuminates					1802:1812	illuminates	1802:1812	illuminates potential targets for barrier manipulation in vivo	1802:1863	This study identifies specific components of the mechanosensing complex associated with the BBB response to fluid shear stress and, therefore, illuminates potential targets for barrier manipulation in vivo.					
35436025	7	29	theme	RhoA	1500:1503	arg1	activation					1505:1514	RhoA activation	1500:1514	RhoA activation	1500:1514	Manipulation of small GTPase activity reveals CD44 activates Rac1 while inhibiting RhoA activation.					
35436025	9	30	theme	fluid	1767:1771	arg1	stress					1779:1784	fluid shear stress	1767:1784	fluid shear stress	1767:1784	This study identifies specific components of the mechanosensing complex associated with the BBB response to fluid shear stress and, therefore, illuminates potential targets for barrier manipulation in vivo.					
35436025	1	31	theme	vascular	172:179	arg1	permeability					181:192	vascular permeability	172:192	vascular permeability	172:192	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	0	32	theme	stress	20:25	arg1	mechanotransduction					27:45	shear stress mechanotransduction	14:45	shear stress mechanotransduction in an in vitro blood-brain barrier model	14:86	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	1	33	theme	blood-brain	262:272	arg1	barrier					274:280	the blood-brain barrier	258:280	the blood-brain barrier (BBB)	258:286	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	1	33	theme	blood-brain	262:272	arg1	BBB					283:285	BBB	283:285	BBB	283:285	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	4	34	theme	electrical	900:909	arg1	resistance					911:920	a transendothelial electrical resistance	881:920	a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays	881:983	The in vitro model used in this study is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays.					
35436025	8	35	theme	shear	1585:1589	arg1	stress					1591:1596	shear stress	1585:1596	shear stress	1585:1596	Additionally, adducin-γ localizes to tight junctions in response to shear stress and RhoA inhibition and is required to maintain the barrier.					
35436025	6	36	theme	Gene	1235:1238	arg1	editing					1240:1246	Gene editing	1235:1246	Gene editing to modulate the expression of CD44, a mechanosensitive receptor for hyaluronan,	1235:1326	Gene editing to modulate the expression of CD44, a mechanosensitive receptor for hyaluronan, demonstrates that the receptor is required for the endothelial response to shear stress.					
35436025	1	37	theme	permeability	181:192	arg1	stress					137:142	Fluid shear stress	125:142	Fluid shear stress	125:142	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	1	37	theme	permeability	181:192	arg1	mediator					160:167	an important mediator	147:167	an important mediator of vascular permeability	147:192	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	0	38	theme	shear	14:18	arg1	mechanotransduction					27:45	shear stress mechanotransduction	14:45	shear stress mechanotransduction in an in vitro blood-brain barrier model	14:86	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	5	39	theme	molecular	1178:1186	arg1	weight					1188:1193	high molecular weight	1173:1193	high molecular weight hyaluronan	1173:1204	These experiments reveal that there is a threshold level of shear stress required for barrier formation and that the composition of the extracellular matrix, specifically the presence of high molecular weight hyaluronan, dictates the flow response.					
35436025	2	40	theme	pathologies	507:517	arg1	understanding					490:502	a better understanding	481:502	a better understanding of pathologies associated with changes in cerebral perfusion including ischemic stroke	481:589	The goal of this study is to probe components of shear mechanotransduction within the BBB to gain a better understanding of pathologies associated with changes in cerebral perfusion including ischemic stroke.					
35436025	3	41	theme	stress	627:632	arg1	effects					610:616	the effects	606:616	the effects of shear stress	606:632	Interrogating the effects of shear stress in vivo is complicated by the complexity of factors in the brain parenchyma and the difficulty associated with modulating blood flow regimes.					
35436025	8	42	theme	tight	1554:1558	arg1	junctions					1560:1568	tight junctions	1554:1568	tight junctions	1554:1568	Additionally, adducin-γ localizes to tight junctions in response to shear stress and RhoA inhibition and is required to maintain the barrier.					
35436025	2	43	theme	shear	432:436	arg1	mechanotransduction					438:456	shear mechanotransduction	432:456	shear mechanotransduction	432:456	The goal of this study is to probe components of shear mechanotransduction within the BBB to gain a better understanding of pathologies associated with changes in cerebral perfusion including ischemic stroke.					
35436025	5	44	theme	weight	1188:1193	arg1	hyaluronan					1195:1204	high molecular weight hyaluronan	1173:1204	high molecular weight hyaluronan	1173:1204	These experiments reveal that there is a threshold level of shear stress required for barrier formation and that the composition of the extracellular matrix, specifically the presence of high molecular weight hyaluronan, dictates the flow response.					
35436025	1	45	from	effect	239:244	arg1	barrier					274:280	the blood-brain barrier	258:280	the blood-brain barrier (BBB)	258:286	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	1	45	from	effect	239:244	arg1	BBB					283:285	BBB	283:285	BBB	283:285	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	0	46	dep	in	53:54	arg1	vitro					56:60	vitro	56:60	vitro	56:60	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	3	47	from	complexity	664:673	arg1	parenchyma					699:708	the brain parenchyma	689:708	the brain parenchyma	689:708	Interrogating the effects of shear stress in vivo is complicated by the complexity of factors in the brain parenchyma and the difficulty associated with modulating blood flow regimes.					
35436025	3	48	theme	blood	756:760	arg1	flow					762:765	blood flow	756:765	blood flow regimes	756:773	Interrogating the effects of shear stress in vivo is complicated by the complexity of factors in the brain parenchyma and the difficulty associated with modulating blood flow regimes.					
35436025	9	49	theme	shear	1773:1777	arg1	stress					1779:1784	fluid shear stress	1767:1784	fluid shear stress	1767:1784	This study identifies specific components of the mechanosensing complex associated with the BBB response to fluid shear stress and, therefore, illuminates potential targets for barrier manipulation in vivo.					
35436025	1	50	theme	brain	364:368	arg1	homeostasis					370:380	brain homeostasis	364:380	brain homeostasis	364:380	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	2	51	theme	study	400:404	arg1	goal					387:390	The goal	383:390	The goal of this study	383:404	The goal of this study is to probe components of shear mechanotransduction within the BBB to gain a better understanding of pathologies associated with changes in cerebral perfusion including ischemic stroke.					
35436025	0	52	theme	in	53:54	arg1	model					82:86	an in vitro blood-brain barrier model	50:86	an in vitro blood-brain barrier model	50:86	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	2	53	theme	better	483:488	arg1	understanding					490:502	a better understanding	481:502	a better understanding of pathologies associated with changes in cerebral perfusion including ischemic stroke	481:589	The goal of this study is to probe components of shear mechanotransduction within the BBB to gain a better understanding of pathologies associated with changes in cerebral perfusion including ischemic stroke.					
35436025	2	54	theme	cerebral	546:553	arg1	perfusion					555:563	cerebral perfusion	546:563	cerebral perfusion including ischemic stroke	546:589	The goal of this study is to probe components of shear mechanotransduction within the BBB to gain a better understanding of pathologies associated with changes in cerebral perfusion including ischemic stroke.					
35436025	3	55	theme	shear	621:625	arg1	stress					627:632	shear stress	621:632	shear stress	621:632	Interrogating the effects of shear stress in vivo is complicated by the complexity of factors in the brain parenchyma and the difficulty associated with modulating blood flow regimes.					
35436025	7	56	theme	activity	1446:1453	arg1	Manipulation					1417:1428	Manipulation	1417:1428	Manipulation of small GTPase activity	1417:1453	Manipulation of small GTPase activity reveals CD44 activates Rac1 while inhibiting RhoA activation.					
35436025	6	57	theme	mechanosensitive	1286:1301	arg1	receptor					1303:1310	a mechanosensitive receptor	1284:1310	a mechanosensitive receptor for hyaluronan	1284:1325	Gene editing to modulate the expression of CD44, a mechanosensitive receptor for hyaluronan, demonstrates that the receptor is required for the endothelial response to shear stress.					
35436025	6	57	theme	mechanosensitive	1286:1301	arg1	CD44					1278:1281	CD44	1278:1281	CD44	1278:1281	Gene editing to modulate the expression of CD44, a mechanosensitive receptor for hyaluronan, demonstrates that the receptor is required for the endothelial response to shear stress.					
35436025	3	58	from	factors	678:684	arg1	parenchyma					699:708	the brain parenchyma	689:708	the brain parenchyma	689:708	Interrogating the effects of shear stress in vivo is complicated by the complexity of factors in the brain parenchyma and the difficulty associated with modulating blood flow regimes.					
35436025	4	59	theme	function	866:873	arg1	measurement					843:853	real-time measurement	833:853	real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays	833:983	The in vitro model used in this study is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays.					
35436025	4	60	theme	barrier	858:864	arg1	function					866:873	barrier function	858:873	barrier function	858:873	The in vitro model used in this study is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays.					
35436025	5	61	theme	threshold	1027:1035	arg1	level					1037:1041	a threshold level	1025:1041	a threshold level of shear stress required for barrier formation	1025:1088	These experiments reveal that there is a threshold level of shear stress required for barrier formation and that the composition of the extracellular matrix, specifically the presence of high molecular weight hyaluronan, dictates the flow response.					
35436025	5	62	theme	barrier	1072:1078	arg1	formation					1080:1088	barrier formation	1072:1088	barrier formation	1072:1088	These experiments reveal that there is a threshold level of shear stress required for barrier formation and that the composition of the extracellular matrix, specifically the presence of high molecular weight hyaluronan, dictates the flow response.					
35436025	9	63	theme	mechanosensing	1708:1721	arg1	complex					1723:1729	the mechanosensing complex	1704:1729	the mechanosensing complex associated with the BBB response to fluid shear stress	1704:1784	This study identifies specific components of the mechanosensing complex associated with the BBB response to fluid shear stress and, therefore, illuminates potential targets for barrier manipulation in vivo.					
35436025	6	64	theme	CD44	1278:1281	arg1	expression					1264:1273	the expression	1260:1273	the expression of CD44, a mechanosensitive receptor for hyaluronan,	1260:1326	Gene editing to modulate the expression of CD44, a mechanosensitive receptor for hyaluronan, demonstrates that the receptor is required for the endothelial response to shear stress.					
35436025	6	65	theme	endothelial	1379:1389	arg1	response					1391:1398	the endothelial response	1375:1398	the endothelial response to shear stress	1375:1414	Gene editing to modulate the expression of CD44, a mechanosensitive receptor for hyaluronan, demonstrates that the receptor is required for the endothelial response to shear stress.					
35436025	4	66	dep	in	780:781	arg1	vitro					783:787	vitro	783:787	vitro	783:787	The in vitro model used in this study is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays.					
35436025	1	67	theme	molecular	203:211	arg1	mechanisms					213:222	the molecular mechanisms	199:222	the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB)	199:286	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	6	68	dep	editing	1240:1246	arg1	modulate					1251:1258	modulate	1251:1258	to modulate the expression of CD44, a mechanosensitive receptor for hyaluronan,	1248:1326	Gene editing to modulate the expression of CD44, a mechanosensitive receptor for hyaluronan, demonstrates that the receptor is required for the endothelial response to shear stress.					
35436025	5	69	theme	shear	1046:1050	arg1	stress					1052:1057	shear stress	1046:1057	shear stress	1046:1057	These experiments reveal that there is a threshold level of shear stress required for barrier formation and that the composition of the extracellular matrix, specifically the presence of high molecular weight hyaluronan, dictates the flow response.					
35436025	9	70	theme	complex	1723:1729	arg1	components					1690:1699	specific components	1681:1699	specific components of the mechanosensing complex associated with the BBB response to fluid shear stress	1681:1784	This study identifies specific components of the mechanosensing complex associated with the BBB response to fluid shear stress and, therefore, illuminates potential targets for barrier manipulation in vivo.					
35436025	1	71	theme	Fluid	125:129	arg1	stress					137:142	Fluid shear stress	125:142	Fluid shear stress	125:142	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	1	71	theme	Fluid	125:129	arg1	mediator					160:167	an important mediator	147:167	an important mediator of vascular permeability	147:192	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	4	72	theme	transendothelial	883:898	arg1	resistance					911:920	a transendothelial electrical resistance	881:920	a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays	881:983	The in vitro model used in this study is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays.					
35436025	0	73	theme	blood-brain	62:72	arg1	model					82:86	an in vitro blood-brain barrier model	50:86	an in vitro blood-brain barrier model	50:86	CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1.					
35436025	5	74	theme	stress	1052:1057	arg1	level					1037:1041	a threshold level	1025:1041	a threshold level of shear stress required for barrier formation	1025:1088	These experiments reveal that there is a threshold level of shear stress required for barrier formation and that the composition of the extracellular matrix, specifically the presence of high molecular weight hyaluronan, dictates the flow response.					
35436025	4	75	theme	in	780:781	arg1	model					789:793	The in vitro model	776:793	The in vitro model used in this study	776:812	The in vitro model used in this study is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays.					
35436025	4	75	theme	in	780:781	arg1	compatible					817:826	compatible	817:826	compatible	817:826	The in vitro model used in this study is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays.					
35436025	1	76	theme	shear	131:135	arg1	stress					137:142	Fluid shear stress	125:142	Fluid shear stress	125:142	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	1	76	theme	shear	131:135	arg1	mediator					160:167	an important mediator	147:167	an important mediator of vascular permeability	147:192	Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.					
35436025	4	77	theme	dextran	957:963	arg1	assays					978:983	immunocytochemistry and dextran permeability assays	933:983	a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays	881:983	The in vitro model used in this study is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays.					
35436025	3	78	from	parenchyma	699:708	arg1	difficulty					718:727	the difficulty	714:727	the difficulty associated with modulating blood flow regimes	714:773	Interrogating the effects of shear stress in vivo is complicated by the complexity of factors in the brain parenchyma and the difficulty associated with modulating blood flow regimes.					
35436025	3	78	from	parenchyma	699:708	arg1	complexity					664:673	the complexity	660:673	the complexity of factors in the brain parenchyma	660:708	Interrogating the effects of shear stress in vivo is complicated by the complexity of factors in the brain parenchyma and the difficulty associated with modulating blood flow regimes.					
35436025	5	79	theme	flow	1220:1223	arg1	response					1225:1232	the flow response	1216:1232	the flow response	1216:1232	These experiments reveal that there is a threshold level of shear stress required for barrier formation and that the composition of the extracellular matrix, specifically the presence of high molecular weight hyaluronan, dictates the flow response.					
35436025	4	80	with	compatible	817:826	arg1	measurement					843:853	real-time measurement	833:853	real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays	833:983	The in vitro model used in this study is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays.					
35436025	3	81	theme	flow	762:765	arg1	regimes					767:773	blood flow regimes	756:773	blood flow regimes	756:773	Interrogating the effects of shear stress in vivo is complicated by the complexity of factors in the brain parenchyma and the difficulty associated with modulating blood flow regimes.					
35072812	4	0	theme	present	558:564	arg1	work					566:569	the present work	554:569	the present work	554:569	In the present work, the chitosan/collagen/hydroxyapatite membranes were prepared with different concentrations of collagen and hydroxyapatite.					
35072812	8	1	with	membranes	1186:1194	arg1	hydroxyapatite					1213:1226	hydroxyapatite	1213:1226	hydroxyapatite	1213:1226	The highest cell adhesion after 48 h was obtained for chitosan membranes with the highest hydroxyapatite and collagen content.					
35072812	8	1	with	membranes	1186:1194	arg1	content					1241:1247	collagen content	1232:1247	collagen content	1232:1247	The highest cell adhesion after 48 h was obtained for chitosan membranes with the highest hydroxyapatite and collagen content.					
35072812	5	2	theme	Cell	695:698	arg1	adhesion					700:707	Cell adhesion	695:707	Cell adhesion	695:707	Cell adhesion was evaluated by MTS assay for two in vitro models.					
35072812	4	3	theme	collagen	666:673	arg1	concentrations					648:661	different concentrations	638:661	different concentrations of collagen and hydroxyapatite	638:692	In the present work, the chitosan/collagen/hydroxyapatite membranes were prepared with different concentrations of collagen and hydroxyapatite.					
35072812	5	4	theme	vitro	747:751	arg1	models					753:758	vitro models	747:758	vitro models	747:758	Cell adhesion was evaluated by MTS assay for two in vitro models.					
35072812	7	5	theme	thermal	1058:1064	arg1	properties					1076:1085	thermal stability properties	1058:1085	thermal stability properties	1058:1085	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	9	6	theme	tissue	1414:1419	arg1	engineering					1421:1431	tissue engineering	1414:1431	tissue engineering	1414:1431	All composite membranes showed good cell adhesion and low cytotoxicity, suggesting that these materials have a significant potential to be used as biomaterials for tissue engineering.					
35072812	6	7	theme	membranes	805:813	arg1	cytotoxicity					775:786	cytotoxicity	775:786	cytotoxicity of the different membranes employing hemolysis of erythrocytes isolated from human blood	775:875	Additionally, cytotoxicity of the different membranes employing hemolysis of erythrocytes isolated from human blood was carried out.					
35072812	4	8	theme	hydroxyapatite	679:692	arg1	concentrations					648:661	different concentrations	638:661	different concentrations of collagen and hydroxyapatite	638:692	In the present work, the chitosan/collagen/hydroxyapatite membranes were prepared with different concentrations of collagen and hydroxyapatite.					
35072812	3	9	theme	phosphates	393:402	arg1	collagen					404:411	calcium phosphates collagen	385:411	calcium phosphates collagen	385:411	Chitosan, calcium phosphates collagen, and their combination as composite materials fulfill the required properties and could result in biostimulation for tissue regeneration.					
35072812	8	10	theme	highest	1127:1133	arg1	adhesion					1140:1147	The highest cell adhesion	1123:1147	The highest cell adhesion after 48 h	1123:1158	The highest cell adhesion after 48 h was obtained for chitosan membranes with the highest hydroxyapatite and collagen content.					
35072812	9	11	contain	have	1354:1357	arg1	materials					1344:1352	these materials	1338:1352	these materials	1338:1352	All composite membranes showed good cell adhesion and low cytotoxicity, suggesting that these materials have a significant potential to be used as biomaterials for tissue engineering.					
35072812	9	11	contain	have	1354:1357	arg2	potential					1373:1381	a significant potential	1359:1381	a significant potential to be used as biomaterials for tissue engineering	1359:1431	All composite membranes showed good cell adhesion and low cytotoxicity, suggesting that these materials have a significant potential to be used as biomaterials for tissue engineering.					
35072812	6	12	theme	different	795:803	arg1	membranes					805:813	the different membranes	791:813	the different membranes employing hemolysis of erythrocytes isolated from human blood	791:875	Additionally, cytotoxicity of the different membranes employing hemolysis of erythrocytes isolated from human blood was carried out.					
35072812	1	13	theme	different	209:217	arg1	types					219:223	different types	209:223	different types of connective tissues	209:245	Tissue engineering is growing in developing new technologies focused on providing effective solutions to degenerative pathologies that affect different types of connective tissues.					
35072812	5	14	theme	MTS	726:728	arg1	assay					730:734	MTS assay	726:734	MTS assay	726:734	Cell adhesion was evaluated by MTS assay for two in vitro models.					
35072812	7	15	theme	scanning	1018:1025	arg1	SEM					1048:1050	SEM	1048:1050	SEM	1048:1050	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	7	15	theme	scanning	1018:1025	arg1	microscopy					1036:1045	scanning electron microscopy	1018:1045	scanning electron microscopy (SEM)	1018:1051	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	2	16	theme	multifunctional	308:322	arg1	materials					324:332	biocompatible, bioactive, biodegradable, and multifunctional materials	263:332	biocompatible, bioactive, biodegradable, and multifunctional materials	263:332	The search for biocompatible, bioactive, biodegradable, and multifunctional materials has grown significantly in recent years.					
35072812	7	17	theme	X-rays	941:946	arg1	diffraction					948:958	X-rays diffraction	941:958	X-rays diffraction (XRD)	941:964	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	7	17	theme	X-rays	941:946	arg1	XRD					961:963	XRD	961:963	XRD	961:963	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	7	18	theme	electron	1027:1034	arg1	SEM					1048:1050	SEM	1048:1050	SEM	1048:1050	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	7	18	theme	electron	1027:1034	arg1	microscopy					1036:1045	scanning electron microscopy	1018:1045	scanning electron microscopy (SEM)	1018:1051	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	1	19	theme	new	111:113	arg1	technologies					115:126	new technologies	111:126	new technologies focused on providing effective solutions to degenerative pathologies that affect different types of connective tissues	111:245	Tissue engineering is growing in developing new technologies focused on providing effective solutions to degenerative pathologies that affect different types of connective tissues.					
35072812	3	20	theme	composite	439:447	arg1	materials					449:457	composite materials	439:457	composite materials	439:457	Chitosan, calcium phosphates collagen, and their combination as composite materials fulfill the required properties and could result in biostimulation for tissue regeneration.					
35072812	4	21	theme	different	638:646	arg1	concentrations					648:661	different concentrations	638:661	different concentrations of collagen and hydroxyapatite	638:692	In the present work, the chitosan/collagen/hydroxyapatite membranes were prepared with different concentrations of collagen and hydroxyapatite.					
35072812	8	22	theme	cell	1135:1138	arg1	adhesion					1140:1147	The highest cell adhesion	1123:1147	The highest cell adhesion after 48 h	1123:1158	The highest cell adhesion after 48 h was obtained for chitosan membranes with the highest hydroxyapatite and collagen content.					
35072812	7	23	dep	Fourier	970:976	arg1	transform					978:986	transform	978:986	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA)	978:1120	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	2	24	theme	recent	361:366	arg1	years					368:372	recent years	361:372	recent years	361:372	The search for biocompatible, bioactive, biodegradable, and multifunctional materials has grown significantly in recent years.					
35072812	1	25	theme	connective	228:237	arg1	tissues					239:245	connective tissues	228:245	connective tissues	228:245	Tissue engineering is growing in developing new technologies focused on providing effective solutions to degenerative pathologies that affect different types of connective tissues.					
35072812	0	26	theme	tissue	47:52	arg1	engineering					54:64	tissue engineering	47:64	tissue engineering	47:64	Chitosan-collagen-hydroxyapatite membranes for tissue engineering.					
35072812	2	27	theme	biodegradable	289:301	arg1	materials					324:332	biocompatible, bioactive, biodegradable, and multifunctional materials	263:332	biocompatible, bioactive, biodegradable, and multifunctional materials	263:332	The search for biocompatible, bioactive, biodegradable, and multifunctional materials has grown significantly in recent years.					
35072812	7	28	dep	transform	978:986	arg1	infrared					988:995	infrared	988:995	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA)	978:1120	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	9	29	theme	composite	1254:1262	arg1	membranes					1264:1272	All composite membranes	1250:1272	All composite membranes	1250:1272	All composite membranes showed good cell adhesion and low cytotoxicity, suggesting that these materials have a significant potential to be used as biomaterials for tissue engineering.					
35072812	1	30	theme	tissues	239:245	arg1	types					219:223	different types	209:223	different types of connective tissues	209:245	Tissue engineering is growing in developing new technologies focused on providing effective solutions to degenerative pathologies that affect different types of connective tissues.					
35072812	7	31	theme	membranes	915:923	arg1	structure					898:906	The structure	894:906	The structure of the membranes	894:923	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	3	32	theme	tissue	530:535	arg1	regeneration					537:548	tissue regeneration	530:548	tissue regeneration	530:548	Chitosan, calcium phosphates collagen, and their combination as composite materials fulfill the required properties and could result in biostimulation for tissue regeneration.					
35072812	10	33	dep	abstract	1444:1451	arg1	Graphical					1434:1442	Graphical	1434:1442	Graphical	1434:1442	Graphical abstract.					
35072812	2	34	theme	bioactive	278:286	arg1	materials					324:332	biocompatible, bioactive, biodegradable, and multifunctional materials	263:332	biocompatible, bioactive, biodegradable, and multifunctional materials	263:332	The search for biocompatible, bioactive, biodegradable, and multifunctional materials has grown significantly in recent years.					
35072812	3	35	theme	required	471:478	arg1	properties					480:489	the required properties	467:489	the required properties	467:489	Chitosan, calcium phosphates collagen, and their combination as composite materials fulfill the required properties and could result in biostimulation for tissue regeneration.					
35072812	7	36	theme	thermogravimetric	1090:1106	arg1	TGA					1117:1119	TGA	1117:1119	TGA	1117:1119	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	7	36	theme	thermogravimetric	1090:1106	arg1	methods					1108:1114	thermogravimetric methods	1090:1114	thermogravimetric methods (TGA)	1090:1120	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	6	37	attach	isolated	851:858	arg2	erythrocytes					838:849	erythrocytes	838:849	erythrocytes isolated from human blood	838:875	Additionally, cytotoxicity of the different membranes employing hemolysis of erythrocytes isolated from human blood was carried out.					
35072812	6	37	attach	isolated	851:858	arg1	blood					871:875	human blood	865:875	human blood	865:875	Additionally, cytotoxicity of the different membranes employing hemolysis of erythrocytes isolated from human blood was carried out.					
35072812	4	38	theme	chitosan/collagen/hydroxyapatite	576:607	arg1	membranes					609:617	the chitosan/collagen/hydroxyapatite membranes	572:617	the chitosan/collagen/hydroxyapatite membranes	572:617	In the present work, the chitosan/collagen/hydroxyapatite membranes were prepared with different concentrations of collagen and hydroxyapatite.					
35072812	8	39	theme	chitosan	1177:1184	arg1	membranes					1186:1194	chitosan membranes	1177:1194	chitosan membranes with the highest hydroxyapatite and collagen content	1177:1247	The highest cell adhesion after 48 h was obtained for chitosan membranes with the highest hydroxyapatite and collagen content.					
35072812	2	40	theme	biocompatible	263:275	arg1	materials					324:332	biocompatible, bioactive, biodegradable, and multifunctional materials	263:332	biocompatible, bioactive, biodegradable, and multifunctional materials	263:332	The search for biocompatible, bioactive, biodegradable, and multifunctional materials has grown significantly in recent years.					
35072812	6	41	theme	human	865:869	arg1	blood					871:875	human blood	865:875	human blood	865:875	Additionally, cytotoxicity of the different membranes employing hemolysis of erythrocytes isolated from human blood was carried out.					
35072812	5	42	from	two	740:742	arg1	models					753:758	vitro models	747:758	vitro models	747:758	Cell adhesion was evaluated by MTS assay for two in vitro models.					
35072812	8	43	dep	hydroxyapatite	1213:1226	arg1	the					1201:1203	the	1201:1203	the	1201:1203	The highest cell adhesion after 48 h was obtained for chitosan membranes with the highest hydroxyapatite and collagen content.					
35072812	1	44	theme	effective	149:157	arg1	solutions					159:167	effective solutions	149:167	effective solutions	149:167	Tissue engineering is growing in developing new technologies focused on providing effective solutions to degenerative pathologies that affect different types of connective tissues.					
35072812	9	45	theme	low	1304:1306	arg1	cytotoxicity					1308:1319	low cytotoxicity	1304:1319	low cytotoxicity	1304:1319	All composite membranes showed good cell adhesion and low cytotoxicity, suggesting that these materials have a significant potential to be used as biomaterials for tissue engineering.					
35072812	9	46	theme	cell	1286:1289	arg1	adhesion					1291:1298	good cell adhesion	1281:1298	good cell adhesion	1281:1298	All composite membranes showed good cell adhesion and low cytotoxicity, suggesting that these materials have a significant potential to be used as biomaterials for tissue engineering.					
35072812	6	47	theme	erythrocytes	838:849	arg1	hemolysis					825:833	hemolysis	825:833	hemolysis of erythrocytes isolated from human blood	825:875	Additionally, cytotoxicity of the different membranes employing hemolysis of erythrocytes isolated from human blood was carried out.					
35072812	9	48	theme	good	1281:1284	arg1	adhesion					1291:1298	good cell adhesion	1281:1298	good cell adhesion	1281:1298	All composite membranes showed good cell adhesion and low cytotoxicity, suggesting that these materials have a significant potential to be used as biomaterials for tissue engineering.					
35072812	8	49	theme	highest	1205:1211	arg1	hydroxyapatite					1213:1226	hydroxyapatite	1213:1226	hydroxyapatite	1213:1226	The highest cell adhesion after 48 h was obtained for chitosan membranes with the highest hydroxyapatite and collagen content.					
35072812	1	50	theme	Tissue	67:72	arg1	engineering					74:84	Tissue engineering	67:84	Tissue engineering	67:84	Tissue engineering is growing in developing new technologies focused on providing effective solutions to degenerative pathologies that affect different types of connective tissues.					
35072812	7	51	theme	stability	1066:1074	arg1	properties					1076:1085	thermal stability properties	1058:1085	thermal stability properties	1058:1085	The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA).					
35072812	1	52	theme	degenerative	172:183	arg1	pathologies					185:195	degenerative pathologies	172:195	degenerative pathologies that affect different types of connective tissues	172:245	Tissue engineering is growing in developing new technologies focused on providing effective solutions to degenerative pathologies that affect different types of connective tissues.					
35072812	3	53	theme	calcium	385:391	arg1	collagen					404:411	calcium phosphates collagen	385:411	calcium phosphates collagen	385:411	Chitosan, calcium phosphates collagen, and their combination as composite materials fulfill the required properties and could result in biostimulation for tissue regeneration.					
35072812	9	54	theme	significant	1361:1371	arg1	potential					1373:1381	a significant potential	1359:1381	a significant potential to be used as biomaterials for tissue engineering	1359:1431	All composite membranes showed good cell adhesion and low cytotoxicity, suggesting that these materials have a significant potential to be used as biomaterials for tissue engineering.					
35072812	8	55	theme	collagen	1232:1239	arg1	content					1241:1247	collagen content	1232:1247	collagen content	1232:1247	The highest cell adhesion after 48 h was obtained for chitosan membranes with the highest hydroxyapatite and collagen content.					
37048237	9	0	theme	HPI-pullulan	1579:1590	arg1	time					1606:1609	the HPI-pullulan glycosylation time	1575:1609	the HPI-pullulan glycosylation time of 24 h	1575:1617	Moreover, the HPI-pullulan glycosylation time of 24 h showed maximum foaming activity (23.04 ± 0.95%) compared to HPI alone (14.20 ± 1.23%).					
37048237	3	1	theme	incubation	627:636	arg1	temperature					638:648	incubation temperature	627:648	incubation temperature (i.e., 50, 60, 70, 80, and 90 °C)	627:682	The conjugation variables were HPI to pullulan ratio (i.e., 3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time (i.e., 3, 6, 12, 24, and 48 h).					
37048237	3	1	theme	incubation	627:636	arg1	°C					680:681	50, 60, 70, 80, and 90 °C	657:681	50, 60, 70, 80, and 90 °C	657:681	The conjugation variables were HPI to pullulan ratio (i.e., 3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time (i.e., 3, 6, 12, 24, and 48 h).					
37048237	1	2	from	implementation	322:335	arg1	food					340:343	food	340:343	food	340:343	Hemp protein, with its important nutritional and industrial value, has trickled into the aisles of protein demand; however, its poor functional properties have largely limited its implementation in food.					
37048237	3	3	dep	w/w	621:623	arg1	i.e.					587:590	i.e.	587:590	i.e.	587:590	The conjugation variables were HPI to pullulan ratio (i.e., 3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time (i.e., 3, 6, 12, 24, and 48 h).					
37048237	10	4	theme	HPI	1731:1733	arg1	stability					1718:1726	The foaming stability	1706:1726	The foaming stability of HPI (79.61 ± 3.33%)	1706:1749	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	3	5	theme	conjugation	537:547	arg1	variables					549:557	The conjugation variables	533:557	The conjugation variables	533:557	The conjugation variables were HPI to pullulan ratio (i.e., 3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time (i.e., 3, 6, 12, 24, and 48 h).					
37048237	3	5	theme	conjugation	537:547	arg1	HPI					564:566	HPI	564:566	HPI	564:566	The conjugation variables were HPI to pullulan ratio (i.e., 3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time (i.e., 3, 6, 12, 24, and 48 h).					
37048237	1	6	theme	industrial	191:200	arg1	value					202:206	its important nutritional and industrial value	161:206	its important nutritional and industrial value	161:206	Hemp protein, with its important nutritional and industrial value, has trickled into the aisles of protein demand; however, its poor functional properties have largely limited its implementation in food.					
37048237	10	7	dep	WHC	1918:1920	arg1	±					1928:1928	4.41 ± 0.73 versus 9.59 ± 0.36 g/g	1923:1956	±	1928:1928	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	10	7	dep	WHC	1918:1920	arg1	g/g					1954:1956	4.41 ± 0.73 versus 9.59 ± 0.36 g/g	1923:1956	g/g	1954:1956	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	4	8	theme	Native	737:742	arg1	control					762:768	a control	760:768	a control for comparison purposes	760:792	Native HPI was used as a control for comparison purposes.					
37048237	4	8	theme	Native	737:742	arg1	HPI					744:746	Native HPI	737:746	Native HPI	737:746	Native HPI was used as a control for comparison purposes.					
37048237	9	9	theme	foaming	1634:1640	arg1	%					1664:1664	23.04 ± 0.95%	1652:1664	23.04 ± 0.95%	1652:1664	Moreover, the HPI-pullulan glycosylation time of 24 h showed maximum foaming activity (23.04 ± 0.95%) compared to HPI alone (14.20 ± 1.23%).					
37048237	9	9	theme	foaming	1634:1640	arg1	activity					1642:1649	maximum foaming activity	1626:1649	maximum foaming activity (23.04 ± 0.95%)	1626:1665	Moreover, the HPI-pullulan glycosylation time of 24 h showed maximum foaming activity (23.04 ± 0.95%) compared to HPI alone (14.20 ± 1.23%).					
37048237	12	10	theme	food	2323:2326	arg1	systems					2328:2334	complicated food systems	2311:2334	complicated food systems	2311:2334	Overall, our findings support the notion that pullulan conjugation provides further functional attributes to the HPI, thereby broadening its potential implementation in complicated food systems.					
37048237	3	11	theme	3:1	593:595	arg1	w/w					621:623	3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time	593:703	w/w	621:623	The conjugation variables were HPI to pullulan ratio (i.e., 3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time (i.e., 3, 6, 12, 24, and 48 h).					
37048237	2	12	with	coupling	418:425	arg1	polysaccharide					441:454	pullulan polysaccharide	432:454	pullulan polysaccharide	432:454	Herein, we aimed to modify hemp protein isolate (HPI) via glycosylation coupling with pullulan polysaccharide, and we subsequently characterized its structural and functional properties.					
37048237	9	13	theme	±	1658:1658	arg1	%					1664:1664	23.04 ± 0.95%	1652:1664	23.04 ± 0.95%	1652:1664	Moreover, the HPI-pullulan glycosylation time of 24 h showed maximum foaming activity (23.04 ± 0.95%) compared to HPI alone (14.20 ± 1.23%).					
37048237	9	13	theme	±	1658:1658	arg1	activity					1642:1649	maximum foaming activity	1626:1649	maximum foaming activity (23.04 ± 0.95%)	1626:1665	Moreover, the HPI-pullulan glycosylation time of 24 h showed maximum foaming activity (23.04 ± 0.95%) compared to HPI alone (14.20 ± 1.23%).					
37048237	3	14	dep	h	733:733	arg1	i.e.					706:709	i.e.	706:709	i.e.	706:709	The conjugation variables were HPI to pullulan ratio (i.e., 3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time (i.e., 3, 6, 12, 24, and 48 h).					
37048237	1	15	theme	Hemp	142:145	arg1	protein					147:153	Hemp protein	142:153	Hemp protein	142:153	Hemp protein, with its important nutritional and industrial value, has trickled into the aisles of protein demand; however, its poor functional properties have largely limited its implementation in food.					
37048237	10	16	theme	0.36	1949:1952	arg1	g/g					1954:1956	4.41 ± 0.73 versus 9.59 ± 0.36 g/g	1923:1956	g/g	1954:1956	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	4	17	used	used	752:755	arg2	HPI					744:746	Native HPI	737:746	Native HPI	737:746	Native HPI was used as a control for comparison purposes.					
37048237	4	17	used	used	752:755	arg2	control					762:768	a control	760:768	a control for comparison purposes	760:792	Native HPI was used as a control for comparison purposes.					
37048237	8	18	theme	±	1521:1521	arg1	m2/g					1528:1531	118.78 ± 4.48 m2/g	1514:1531	118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g)	1514:1562	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	2	19	theme	structural	495:504	arg1	properties					521:530	its structural and functional properties	491:530	its structural and functional properties	491:530	Herein, we aimed to modify hemp protein isolate (HPI) via glycosylation coupling with pullulan polysaccharide, and we subsequently characterized its structural and functional properties.					
37048237	11	20	theme	correlation	2020:2030	arg1	analysis					2032:2039	correlation analysis	2020:2039	correlation analysis	2020:2039	Intriguingly, correlation analysis showed that protein functional characteristics were significantly and positively correlated with DG.					
37048237	5	21	theme	gel	1055:1057	arg1	top					1036:1038	the top	1032:1038	the top of the running gel	1032:1057	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	0	22	theme	Polysaccharide	75:88	arg1	Conjugate					104:112	Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate	45:112	Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate	45:112	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.					
37048237	8	23	dep	significantly	1393:1405	arg1	<					1410:1410	p < 0.05	1408:1415	p < 0.05	1408:1415	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	2	24	theme	glycosylation	404:416	arg1	coupling					418:425	glycosylation coupling	404:425	glycosylation coupling with pullulan polysaccharide	404:454	Herein, we aimed to modify hemp protein isolate (HPI) via glycosylation coupling with pullulan polysaccharide, and we subsequently characterized its structural and functional properties.					
37048237	8	25	theme	m2/g	1528:1531	arg1	activity					1502:1509	maximum emulsifying activity	1482:1509	maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g)	1482:1562	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	11	26	theme	protein	2053:2059	arg1	characteristics					2072:2086	protein functional characteristics	2053:2086	protein functional characteristics	2053:2086	Intriguingly, correlation analysis showed that protein functional characteristics were significantly and positively correlated with DG.					
37048237	12	27	theme	functional	2226:2235	arg1	attributes					2237:2246	further functional attributes	2218:2246	further functional attributes	2218:2246	Overall, our findings support the notion that pullulan conjugation provides further functional attributes to the HPI, thereby broadening its potential implementation in complicated food systems.					
37048237	0	28	theme	Conjugate	104:112	arg1	Characteristics					26:40	Structural and Functional Characteristics	0:40	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.	0:140	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.					
37048237	12	29	theme	further	2218:2224	arg1	attributes					2237:2246	further functional attributes	2218:2246	further functional attributes	2218:2246	Overall, our findings support the notion that pullulan conjugation provides further functional attributes to the HPI, thereby broadening its potential implementation in complicated food systems.					
37048237	2	30	theme	functional	510:519	arg1	properties					521:530	its structural and functional properties	491:530	its structural and functional properties	491:530	Herein, we aimed to modify hemp protein isolate (HPI) via glycosylation coupling with pullulan polysaccharide, and we subsequently characterized its structural and functional properties.					
37048237	9	31	theme	±	1696:1696	arg1	%					1702:1702	14.20 ± 1.23%	1690:1702	14.20 ± 1.23%	1690:1702	Moreover, the HPI-pullulan glycosylation time of 24 h showed maximum foaming activity (23.04 ± 0.95%) compared to HPI alone (14.20 ± 1.23%).					
37048237	9	31	theme	±	1696:1696	arg1	HPI					1679:1681	HPI	1679:1681	HPI alone (14.20 ± 1.23%)	1679:1703	Moreover, the HPI-pullulan glycosylation time of 24 h showed maximum foaming activity (23.04 ± 0.95%) compared to HPI alone (14.20 ± 1.23%).					
37048237	0	32	theme	Model	128:132	arg1	System					134:139	an Aqueous Model System	117:139	an Aqueous Model System	117:139	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.					
37048237	2	33	theme	pullulan	432:439	arg1	polysaccharide					441:454	pullulan polysaccharide	432:454	pullulan polysaccharide	432:454	Herein, we aimed to modify hemp protein isolate (HPI) via glycosylation coupling with pullulan polysaccharide, and we subsequently characterized its structural and functional properties.					
37048237	10	34	theme	8.48	1968:1971	arg1	±					1973:1973	8.48 ± 0.51 versus 13.73 ± 0.59 g/g	1968:2002	±	1973:1973	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	1	35	dep	important	165:173	arg1	nutritional					175:185	nutritional	175:185	nutritional	175:185	Hemp protein, with its important nutritional and industrial value, has trickled into the aisles of protein demand; however, its poor functional properties have largely limited its implementation in food.					
37048237	2	36	theme	hemp	373:376	arg1	HPI					395:397	HPI	395:397	HPI	395:397	Herein, we aimed to modify hemp protein isolate (HPI) via glycosylation coupling with pullulan polysaccharide, and we subsequently characterized its structural and functional properties.					
37048237	2	36	theme	hemp	373:376	arg1	isolate					386:392	hemp protein isolate	373:392	hemp protein isolate (HPI)	373:398	Herein, we aimed to modify hemp protein isolate (HPI) via glycosylation coupling with pullulan polysaccharide, and we subsequently characterized its structural and functional properties.					
37048237	0	37	theme	Structural	0:9	arg1	Characteristics					26:40	Structural and Functional Characteristics	0:40	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.	0:140	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.					
37048237	1	38	theme	poor	270:273	arg1	properties					286:295	its poor functional properties	266:295	its poor functional properties	266:295	Hemp protein, with its important nutritional and industrial value, has trickled into the aisles of protein demand; however, its poor functional properties have largely limited its implementation in food.					
37048237	5	39	dep	°C.	920:922	arg1	shows					942:946	shows	942:946	shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control	942:1109	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	0	40	theme	Functional	15:24	arg1	Characteristics					26:40	Structural and Functional Characteristics	0:40	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.	0:140	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.					
37048237	5	41	theme	SDS-PAGE	924:931	arg1	analysis					933:940	SDS-PAGE analysis	924:940	SDS-PAGE analysis	924:940	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	6	42	dep	secondary	1158:1166	arg1	structures					1181:1190	structures	1181:1190	structures	1181:1190	Further, glycosylation could loosen the HPI's secondary and tertiary structures, as well as increase surface hydrophobicity.					
37048237	8	43	with	glycosylation	1424:1436	arg1	HPI-pullulan					1443:1454	HPI-pullulan	1443:1454	HPI-pullulan	1443:1454	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	0	44	theme	Protein	50:56	arg1	Conjugate					104:112	Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate	45:112	Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate	45:112	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.					
37048237	8	45	dep	m2/g	1528:1531	arg1	HPI					1534:1536	HPI	1534:1536	HPI alone: 32.38 ± 3.65 m2/g	1534:1561	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	5	46	theme	heavier	989:995	arg1	bands					1014:1018	wider and heavier molecular weight bands	979:1018	wider and heavier molecular weight bands	979:1018	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	10	47	theme	foaming	1710:1716	arg1	stability					1718:1726	The foaming stability	1706:1726	The foaming stability of HPI (79.61 ± 3.33%)	1706:1749	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	1	48	theme	protein	241:247	arg1	demand					249:254	protein demand	241:254	protein demand	241:254	Hemp protein, with its important nutritional and industrial value, has trickled into the aisles of protein demand; however, its poor functional properties have largely limited its implementation in food.					
37048237	9	49	theme	glycosylation	1592:1604	arg1	time					1606:1609	the HPI-pullulan glycosylation time	1575:1609	the HPI-pullulan glycosylation time of 24 h	1575:1617	Moreover, the HPI-pullulan glycosylation time of 24 h showed maximum foaming activity (23.04 ± 0.95%) compared to HPI alone (14.20 ± 1.23%).					
37048237	8	50	theme	Emulsifying	1363:1373	arg1	activity					1375:1382	Emulsifying activity	1363:1382	Emulsifying activity	1363:1382	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	5	51	theme	wider	979:983	arg1	bands					1014:1018	wider and heavier molecular weight bands	979:1018	wider and heavier molecular weight bands	979:1018	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	9	52	theme	h	1617:1617	arg1	time					1606:1609	the HPI-pullulan glycosylation time	1575:1609	the HPI-pullulan glycosylation time of 24 h	1575:1617	Moreover, the HPI-pullulan glycosylation time of 24 h showed maximum foaming activity (23.04 ± 0.95%) compared to HPI alone (14.20 ± 1.23%).					
37048237	3	53	theme	pullulan	571:578	arg1	ratio					580:584	pullulan ratio	571:584	pullulan ratio	571:584	The conjugation variables were HPI to pullulan ratio (i.e., 3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time (i.e., 3, 6, 12, 24, and 48 h).					
37048237	8	54	gly	glycosylation	1424:1436	arg2	ratio					1461:1465	a ratio	1459:1465	a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g)	1459:1562	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	8	54	gly	glycosylation	1424:1436	arg1	ratio					1461:1465	a ratio	1459:1465	a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g)	1459:1562	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	9	55	theme	maximum	1626:1632	arg1	%					1664:1664	23.04 ± 0.95%	1652:1664	23.04 ± 0.95%	1652:1664	Moreover, the HPI-pullulan glycosylation time of 24 h showed maximum foaming activity (23.04 ± 0.95%) compared to HPI alone (14.20 ± 1.23%).					
37048237	9	55	theme	maximum	1626:1632	arg1	activity					1642:1649	maximum foaming activity	1626:1649	maximum foaming activity (23.04 ± 0.95%)	1626:1665	Moreover, the HPI-pullulan glycosylation time of 24 h showed maximum foaming activity (23.04 ± 0.95%) compared to HPI alone (14.20 ± 1.23%).					
37048237	8	56	dep	HPI	1534:1536	arg1	m2/g					1558:1561	32.38 ± 3.65 m2/g	1545:1561	HPI alone: 32.38 ± 3.65 m2/g	1534:1561	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	4	57	theme	comparison	774:783	arg1	purposes					785:792	comparison purposes	774:792	comparison purposes	774:792	Native HPI was used as a control for comparison purposes.					
37048237	8	58	theme	1:3	1470:1472	arg1	ratio					1461:1465	a ratio	1459:1465	a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g)	1459:1562	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	12	59	theme	complicated	2311:2321	arg1	systems					2328:2334	complicated food systems	2311:2334	complicated food systems	2311:2334	Overall, our findings support the notion that pullulan conjugation provides further functional attributes to the HPI, thereby broadening its potential implementation in complicated food systems.					
37048237	8	60	theme	maximum	1482:1488	arg1	activity					1502:1509	maximum emulsifying activity	1482:1509	maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g)	1482:1562	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	5	61	theme	pullulan	840:847	arg1	greater					866:872	greater	866:872	greater	866:872	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	5	61	theme	pullulan	840:847	arg1	ratio					856:860	the pullulan to HPI ratio	836:860	the pullulan to HPI ratio	836:860	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	10	62	theme	±	1947:1947	arg1	g/g					1954:1956	4.41 ± 0.73 versus 9.59 ± 0.36 g/g	1923:1956	g/g	1954:1956	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	10	63	theme	glycosylation	1819:1831	arg1	temperature					1833:1843	a glycosylation temperature	1817:1843	a glycosylation temperature of 80 °C.	1817:1853	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	5	64	theme	running	1047:1053	arg1	gel					1055:1057	the running gel	1043:1057	the running gel	1043:1057	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	3	65	theme	incubation	689:698	arg1	time					700:703	incubation time	689:703	incubation time	689:703	The conjugation variables were HPI to pullulan ratio (i.e., 3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time (i.e., 3, 6, 12, 24, and 48 h).					
37048237	0	66	theme	Glycosylation	90:102	arg1	Conjugate					104:112	Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate	45:112	Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate	45:112	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.					
37048237	5	67	theme	HPI	852:854	arg1	greater					866:872	greater	866:872	greater	866:872	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	5	67	theme	HPI	852:854	arg1	ratio					856:860	the pullulan to HPI ratio	836:860	the pullulan to HPI ratio	836:860	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	8	68	theme	118.78	1514:1519	arg1	m2/g					1528:1531	118.78 ± 4.48 m2/g	1514:1531	118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g)	1514:1562	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	3	69	dep	HPI	564:566	arg1	h					733:733	3, 6, 12, 24, and 48 h	712:733	3, 6, 12, 24, and 48 h	712:733	The conjugation variables were HPI to pullulan ratio (i.e., 3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time (i.e., 3, 6, 12, 24, and 48 h).					
37048237	0	70	from	Characteristics	26:40	arg1	System					134:139	an Aqueous Model System	117:139	an Aqueous Model System	117:139	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.					
37048237	5	71	from	absent	1089:1094	arg1	control					1103:1109	the control	1099:1109	the control	1099:1109	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	1	72	theme	important	165:173	arg1	value					202:206	its important nutritional and industrial value	161:206	its important nutritional and industrial value	161:206	Hemp protein, with its important nutritional and industrial value, has trickled into the aisles of protein demand; however, its poor functional properties have largely limited its implementation in food.					
37048237	10	73	theme	°C.	1851:1853	arg1	temperature					1833:1843	a glycosylation temperature	1817:1843	a glycosylation temperature of 80 °C.	1817:1853	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	0	74	theme	Aqueous	120:126	arg1	System					134:139	an Aqueous Model System	117:139	an Aqueous Model System	117:139	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.					
37048237	1	75	with	protein	147:153	arg1	value					202:206	its important nutritional and industrial value	161:206	its important nutritional and industrial value	161:206	Hemp protein, with its important nutritional and industrial value, has trickled into the aisles of protein demand; however, its poor functional properties have largely limited its implementation in food.					
37048237	11	76	theme	functional	2061:2070	arg1	characteristics					2072:2086	protein functional characteristics	2053:2086	protein functional characteristics	2053:2086	Intriguingly, correlation analysis showed that protein functional characteristics were significantly and positively correlated with DG.					
37048237	10	77	theme	un-glycated	1873:1883	arg1	HPI					1885:1887	the un-glycated HPI	1869:1887	the un-glycated HPI	1869:1887	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	7	78	dep	increased	1293:1301	arg1	<					1306:1306	p < 0.05	1304:1311	p < 0.05	1304:1311	The solubility of HPI after glycosylation significantly increased (p < 0.05) at pH 7.0 compared to HPI without glycosylation.					
37048237	2	79	theme	protein	378:384	arg1	HPI					395:397	HPI	395:397	HPI	395:397	Herein, we aimed to modify hemp protein isolate (HPI) via glycosylation coupling with pullulan polysaccharide, and we subsequently characterized its structural and functional properties.					
37048237	2	79	theme	protein	378:384	arg1	isolate					386:392	hemp protein isolate	373:392	hemp protein isolate (HPI)	373:398	Herein, we aimed to modify hemp protein isolate (HPI) via glycosylation coupling with pullulan polysaccharide, and we subsequently characterized its structural and functional properties.					
37048237	3	80	dep	°C	680:681	arg1	i.e.					651:654	i.e.	651:654	i.e.	651:654	The conjugation variables were HPI to pullulan ratio (i.e., 3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time (i.e., 3, 6, 12, 24, and 48 h).					
37048237	5	81	from	control	1103:1109	arg1	absent					1089:1094	absent	1089:1094	absent	1089:1094	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	1	82	theme	functional	275:284	arg1	properties					286:295	its poor functional properties	266:295	its poor functional properties	266:295	Hemp protein, with its important nutritional and industrial value, has trickled into the aisles of protein demand; however, its poor functional properties have largely limited its implementation in food.					
37048237	12	83	theme	pullulan	2188:2195	arg1	conjugation					2197:2207	pullulan conjugation	2188:2207	pullulan conjugation	2188:2207	Overall, our findings support the notion that pullulan conjugation provides further functional attributes to the HPI, thereby broadening its potential implementation in complicated food systems.					
37048237	10	84	theme	±	1993:1993	arg1	g/g					2000:2002	8.48 ± 0.51 versus 13.73 ± 0.59 g/g	1968:2002	g/g	2000:2002	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	5	85	theme	such	1066:1069	arg1	observations					1071:1082	such observations	1066:1082	such observations	1066:1082	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	0	86	theme	Hemp	45:48	arg1	Conjugate					104:112	Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate	45:112	Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate	45:112	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.					
37048237	12	87	from	implementation	2293:2306	arg1	systems					2328:2334	complicated food systems	2311:2334	complicated food systems	2311:2334	Overall, our findings support the notion that pullulan conjugation provides further functional attributes to the HPI, thereby broadening its potential implementation in complicated food systems.					
37048237	7	88	theme	p	1304:1304	arg1	<					1306:1306	p < 0.05	1304:1311	p < 0.05	1304:1311	The solubility of HPI after glycosylation significantly increased (p < 0.05) at pH 7.0 compared to HPI without glycosylation.					
37048237	12	89	theme	potential	2283:2291	arg1	implementation					2293:2306	its potential implementation	2279:2306	its potential implementation in complicated food systems	2279:2334	Overall, our findings support the notion that pullulan conjugation provides further functional attributes to the HPI, thereby broadening its potential implementation in complicated food systems.					
37048237	5	90	theme	weight	1007:1012	arg1	bands					1014:1018	wider and heavier molecular weight bands	979:1018	wider and heavier molecular weight bands	979:1018	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	0	91	theme	Isolate-Pullulan	58:73	arg1	Conjugate					104:112	Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate	45:112	Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate	45:112	Structural and Functional Characteristics of Hemp Protein Isolate-Pullulan Polysaccharide Glycosylation Conjugate in an Aqueous Model System.					
37048237	10	92	dep	OHC	1963:1965	arg1	g/g					2000:2002	8.48 ± 0.51 versus 13.73 ± 0.59 g/g	1968:2002	g/g	2000:2002	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	10	92	dep	OHC	1963:1965	arg1	±					1973:1973	8.48 ± 0.51 versus 13.73 ± 0.59 g/g	1968:2002	±	1973:1973	The foaming stability of HPI (79.61 ± 3.33%) increased to 97.78 ± 3.85% when HPI-pullulan was conjugated using a glycosylation temperature of 80 °C. Compared with the un-glycated HPI, HPI-pullulan also increased WHC (4.41 ± 0.73 versus 9.59 ± 0.36 g/g) and OHC (8.48 ± 0.51 versus 13.73 ± 0.59 g/g).					
37048237	8	93	theme	p	1408:1408	arg1	<					1410:1410	p < 0.05	1408:1415	p < 0.05	1408:1415	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	3	94	theme	1:3	617:619	arg1	w/w					621:623	3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time	593:703	w/w	621:623	The conjugation variables were HPI to pullulan ratio (i.e., 3:1, 2:1, 1:1, 1:2, and 1:3 w/w), incubation temperature (i.e., 50, 60, 70, 80, and 90 °C), and incubation time (i.e., 3, 6, 12, 24, and 48 h).					
37048237	8	95	theme	emulsifying	1490:1500	arg1	activity					1502:1509	maximum emulsifying activity	1482:1509	maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g)	1482:1562	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	5	96	theme	molecular	997:1005	arg1	bands					1014:1018	wider and heavier molecular weight bands	979:1018	wider and heavier molecular weight bands	979:1018	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	7	97	theme	HPI	1255:1257	arg1	solubility					1241:1250	The solubility	1237:1250	The solubility of HPI after glycosylation	1237:1277	The solubility of HPI after glycosylation significantly increased (p < 0.05) at pH 7.0 compared to HPI without glycosylation.					
37048237	5	98	dep	ratio	856:860	arg1	to					849:850	to	849:850	to	849:850	We found that DG tended to decrease when the pullulan to HPI ratio was greater than 1:1 and when the temperature exceeded 80 °C. SDS-PAGE analysis shows that when the DG is increased, wider and heavier molecular weight bands emerge near the top of the running gel, while such observations were absent in the control.					
37048237	8	99	from	ratio	1461:1465	arg1	glycosylation					1424:1436	glycosylation	1424:1436	glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g)	1424:1562	Emulsifying activity improved significantly (p < 0.05), with glycosylation with HPI-pullulan at a ratio of 1:3 showing maximum emulsifying activity of 118.78 ± 4.48 m2/g (HPI alone: 32.38 ± 3.65 m2/g).					
37048237	6	100	theme	surface	1213:1219	arg1	hydrophobicity					1221:1234	surface hydrophobicity	1213:1234	surface hydrophobicity	1213:1234	Further, glycosylation could loosen the HPI's secondary and tertiary structures, as well as increase surface hydrophobicity.					
37048237	1	101	theme	demand	249:254	arg1	aisles					231:236	the aisles	227:236	the aisles of protein demand	227:254	Hemp protein, with its important nutritional and industrial value, has trickled into the aisles of protein demand; however, its poor functional properties have largely limited its implementation in food.					
35153297	6	0	theme	Ginsenoside	999:1009	arg1	Rb1					1011:1013	Ginsenoside Rb1	999:1013	Ginsenoside Rb1	999:1013	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	4	1	from	role	510:513	arg1	viability					542:550	cell viability	537:550	cell viability	537:550	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	4	1	from	role	510:513	arg1	activity					612:619	ALP activity	608:619	ALP activity of rat BMSCs	608:632	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	4	1	from	role	510:513	arg1	proliferation					553:565	proliferation	553:565	proliferation	553:565	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	4	1	from	role	510:513	arg1	apoptosis					568:576	apoptosis	568:576	apoptosis	568:576	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	4	1	from	role	510:513	arg1	expression					596:605	osteogenic genes expression	579:605	osteogenic genes expression	579:605	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	3	2	from	ability	451:457	arg1	defect					467:472	bone defect	462:472	bone defect	462:472	Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive.					
35153297	1	3	theme	ginseng	184:190	arg1	Rb1					150:152	Ginsenoside Rb1	138:152	Ginsenoside Rb1	138:152	Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system.					
35153297	1	3	theme	ginseng	184:190	arg1	constituent					169:179	the effective constituent	155:179	the effective constituent of ginseng	155:190	Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system.					
35153297	9	4	theme	angiogenesis	1461:1472	arg1	factor					1474:1479	angiogenesis factor	1461:1479	angiogenesis factor	1461:1479	All the outcomes hint that Ginsenoside Rb1 could reinforce the osteogenesis differentiation and angiogenesis factor's expression of BMSCs.					
35153297	7	5	theme	delivery	1218:1225	arg1	carrier					1227:1233	a delivery carrier	1216:1233	a delivery carrier for Ginsenoside Rb1	1216:1253	Meanwhile, micro-nano HAp granules combined with silk were fabricated smoothly and were a delivery carrier for Ginsenoside Rb1.					
35153297	5	6	theme	calvarial	869:877	arg1	models					886:891	rat calvarial defect models	865:891	rat calvarial defect models	865:891	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	5	7	theme	defect	879:884	arg1	models					886:891	rat calvarial defect models	865:891	rat calvarial defect models	865:891	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	6	8	theme	osteogenic	1084:1093	arg1	viability					1035:1043	cell viability	1030:1043	cell viability	1030:1043	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	6	8	theme	osteogenic	1084:1093	arg1	expression					1116:1125	osteogenic and angiogenic genes expression	1084:1125	osteogenic and angiogenic genes expression	1084:1125	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	6	9	theme	cell	1030:1033	arg1	viability					1035:1043	cell viability	1030:1043	cell viability	1030:1043	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	6	9	theme	cell	1030:1033	arg1	expression					1116:1125	osteogenic and angiogenic genes expression	1084:1125	osteogenic and angiogenic genes expression	1084:1125	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	6	9	theme	cell	1030:1033	arg1	activity					1074:1081	significantly increased ALP activity	1046:1081	significantly increased ALP activity	1046:1081	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	4	10	theme	osteogenic	579:588	arg1	expression					596:605	osteogenic genes expression	579:605	osteogenic genes expression	579:605	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	4	10	theme	osteogenic	579:588	arg1	viability					542:550	cell viability	537:550	cell viability	537:550	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	0	11	theme	calvarial	120:128	arg1	defect					130:135	calvarial defect	120:135	calvarial defect	120:135	The osteogenesis of Ginsenoside Rb1 incorporated silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect.					
35153297	5	12	theme	osteogenic	780:789	arg1	effect					806:811	the osteogenic and angiogenic effect	776:811	the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models	776:891	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	7	13	theme	HAp	1150:1152	arg1	granules					1154:1161	micro-nano HAp granules	1139:1161	micro-nano HAp granules combined with silk	1139:1180	Meanwhile, micro-nano HAp granules combined with silk were fabricated smoothly and were a delivery carrier for Ginsenoside Rb1.					
35153297	7	13	theme	HAp	1150:1152	arg1	Meanwhile					1128:1136	Meanwhile	1128:1136	Meanwhile	1128:1136	Meanwhile, micro-nano HAp granules combined with silk were fabricated smoothly and were a delivery carrier for Ginsenoside Rb1.					
35153297	5	14	theme	histology	948:956	arg1	analysis					958:965	histology analysis	948:965	histology analysis	948:965	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	6	15	theme	genes	1110:1114	arg1	viability					1035:1043	cell viability	1030:1043	cell viability	1030:1043	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	6	15	theme	genes	1110:1114	arg1	expression					1116:1125	osteogenic and angiogenic genes expression	1084:1125	osteogenic and angiogenic genes expression	1084:1125	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	10	16	theme	repair	1594:1599	arg1	defect					1606:1611	repair bone defect	1594:1611	repair bone defect	1594:1611	Moreover, micro-nano HAp combined with silk could act as a carrier for Ginsenoside Rb1 to repair bone defect.					
35153297	8	17	theme	micro-nano	1297:1306	arg1	HAp/silk					1308:1315	micro-nano HAp/silk	1297:1315	micro-nano HAp/silk	1297:1315	Significantly, Ginsenoside Rb1 loaded on micro-nano HAp/silk could facilitate osteogenesis and angiogenesis.					
35153297	10	18	theme	micro-nano	1514:1523	arg1	HAp					1525:1527	micro-nano HAp	1514:1527	micro-nano HAp combined with silk	1514:1546	Moreover, micro-nano HAp combined with silk could act as a carrier for Ginsenoside Rb1 to repair bone defect.					
35153297	10	18	theme	micro-nano	1514:1523	arg1	carrier					1563:1569	a carrier	1561:1569	a carrier for Ginsenoside Rb1 to repair bone defect	1561:1611	Moreover, micro-nano HAp combined with silk could act as a carrier for Ginsenoside Rb1 to repair bone defect.					
35153297	2	19	theme	little	291:296	arg1	study					298:302	little study	291:302	little study	291:302	However, there is little study on the osteogenesis and angiogenesis effect of Ginsenoside Rb1.					
35153297	6	20	theme	increased	1060:1068	arg1	viability					1035:1043	cell viability	1030:1043	cell viability	1030:1043	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	6	20	theme	increased	1060:1068	arg1	activity					1074:1081	significantly increased ALP activity	1046:1081	significantly increased ALP activity	1046:1081	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	3	21	theme	repairment	440:449	arg1	ability					451:457	its repairment ability	436:457	its repairment ability in bone defect	436:472	Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive.					
35153297	7	22	theme	micro-nano	1139:1148	arg1	granules					1154:1161	micro-nano HAp granules	1139:1161	micro-nano HAp granules combined with silk	1139:1180	Meanwhile, micro-nano HAp granules combined with silk were fabricated smoothly and were a delivery carrier for Ginsenoside Rb1.					
35153297	7	22	theme	micro-nano	1139:1148	arg1	Meanwhile					1128:1136	Meanwhile	1128:1136	Meanwhile	1128:1136	Meanwhile, micro-nano HAp granules combined with silk were fabricated smoothly and were a delivery carrier for Ginsenoside Rb1.					
35153297	5	23	theme	Ginsenoside	755:765	arg1	Rb1					767:769	Ginsenoside Rb1	755:769	Ginsenoside Rb1	755:769	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	5	24	theme	sequential	910:919	arg1	labeling					934:941	sequential fluorescence labeling	910:941	sequential fluorescence labeling	910:941	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	6	25	theme	ALP	1070:1072	arg1	viability					1035:1043	cell viability	1030:1043	cell viability	1030:1043	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	6	25	theme	ALP	1070:1072	arg1	activity					1074:1081	significantly increased ALP activity	1046:1081	significantly increased ALP activity	1046:1081	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	10	26	theme	bone	1601:1604	arg1	defect					1606:1611	repair bone defect	1594:1611	repair bone defect	1594:1611	Moreover, micro-nano HAp combined with silk could act as a carrier for Ginsenoside Rb1 to repair bone defect.					
35153297	2	27	theme	angiogenesis	328:339	arg1	effect					341:346	the osteogenesis and angiogenesis effect	307:346	the osteogenesis and angiogenesis effect of Ginsenoside Rb1	307:365	However, there is little study on the osteogenesis and angiogenesis effect of Ginsenoside Rb1.					
35153297	5	28	theme	micro-nano	842:851	arg1	HAp/silk					853:860	micro-nano HAp/silk	842:860	micro-nano HAp/silk in rat calvarial defect models	842:891	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	3	29	theme	delivery	397:404	arg1	system					406:411	a delivery system	395:411	a delivery system of Ginsenoside Rb1	395:430	Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive.					
35153297	5	30	theme	fluorescence	921:932	arg1	labeling					934:941	sequential fluorescence labeling	910:941	sequential fluorescence labeling	910:941	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	9	31	theme	BMSCs	1497:1501	arg1	expression					1483:1492	the osteogenesis differentiation and angiogenesis factor's expression	1424:1492	the osteogenesis differentiation and angiogenesis factor's expression of BMSCs	1424:1501	All the outcomes hint that Ginsenoside Rb1 could reinforce the osteogenesis differentiation and angiogenesis factor's expression of BMSCs.					
35153297	0	32	theme	Ginsenoside	20:30	arg1	Rb1					32:34	Ginsenoside Rb1	20:34	Ginsenoside Rb1	20:34	The osteogenesis of Ginsenoside Rb1 incorporated silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect.					
35153297	4	33	theme	cell	537:540	arg1	viability					542:550	cell viability	537:550	cell viability	537:550	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	4	33	theme	cell	537:540	arg1	activity					612:619	ALP activity	608:619	ALP activity of rat BMSCs	608:632	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	4	33	theme	cell	537:540	arg1	proliferation					553:565	proliferation	553:565	proliferation	553:565	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	4	33	theme	cell	537:540	arg1	apoptosis					568:576	apoptosis	568:576	apoptosis	568:576	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	4	33	theme	cell	537:540	arg1	expression					596:605	osteogenic genes expression	579:605	osteogenic genes expression	579:605	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	2	34	theme	osteogenesis	311:322	arg1	effect					341:346	the osteogenesis and angiogenesis effect	307:346	the osteogenesis and angiogenesis effect of Ginsenoside Rb1	307:365	However, there is little study on the osteogenesis and angiogenesis effect of Ginsenoside Rb1.					
35153297	8	35	theme	Ginsenoside	1271:1281	arg1	Rb1					1283:1285	Ginsenoside Rb1	1271:1285	Ginsenoside Rb1 loaded on micro-nano HAp/silk	1271:1315	Significantly, Ginsenoside Rb1 loaded on micro-nano HAp/silk could facilitate osteogenesis and angiogenesis.					
35153297	5	36	theme	angiogenic	795:804	arg1	effect					806:811	the osteogenic and angiogenic effect	776:811	the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models	776:891	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	1	37	theme	Ginsenoside	138:148	arg1	Rb1					150:152	Ginsenoside Rb1	138:152	Ginsenoside Rb1	138:152	Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system.					
35153297	1	37	theme	Ginsenoside	138:148	arg1	constituent					169:179	the effective constituent	155:179	the effective constituent of ginseng	155:190	Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system.					
35153297	1	38	theme	favorable	223:231	arg1	roles					233:237	favorable roles	223:237	favorable roles	223:237	Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system.					
35153297	3	39	theme	Ginsenoside	416:426	arg1	Rb1					428:430	Ginsenoside Rb1	416:430	Ginsenoside Rb1	416:430	Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive.					
35153297	5	40	theme	Ginsenoside	816:826	arg1	Rb1					828:830	Ginsenoside Rb1	816:830	Ginsenoside Rb1	816:830	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	4	41	theme	BMSCs	628:632	arg1	viability					542:550	cell viability	537:550	cell viability	537:550	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	4	41	theme	BMSCs	628:632	arg1	activity					612:619	ALP activity	608:619	ALP activity of rat BMSCs	608:632	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	0	42	theme	Rb1	32:34	arg1	osteogenesis					4:15	The osteogenesis	0:15	The osteogenesis of Ginsenoside Rb1	0:34	The osteogenesis of Ginsenoside Rb1 incorporated silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect.					
35153297	4	43	theme	Ginsenoside	518:528	arg1	Rb1					530:532	Ginsenoside Rb1	518:532	Ginsenoside Rb1	518:532	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	3	44	theme	Rb1	428:430	arg1	ability					451:457	its repairment ability	436:457	its repairment ability in bone defect	436:472	Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive.					
35153297	3	44	theme	Rb1	428:430	arg1	system					406:411	a delivery system	395:411	a delivery system of Ginsenoside Rb1	395:430	Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive.					
35153297	4	45	theme	ALP	608:610	arg1	viability					542:550	cell viability	537:550	cell viability	537:550	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	4	45	theme	ALP	608:610	arg1	activity					612:619	ALP activity	608:619	ALP activity of rat BMSCs	608:632	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	5	46	theme	Rb1	828:830	arg1	effect					806:811	the osteogenic and angiogenic effect	776:811	the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models	776:891	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	3	47	theme	bone	462:465	arg1	defect					467:472	bone defect	462:472	bone defect	462:472	Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive.					
35153297	0	48	theme	hydroxyapatite/sodium	65:85	arg1	scaffolds					106:114	silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds	49:114	silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect	49:135	The osteogenesis of Ginsenoside Rb1 incorporated silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect.					
35153297	5	49	theme	delivery	736:743	arg1	system					745:750	a delivery system	734:750	a delivery system of Ginsenoside Rb1	734:769	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	2	50	theme	Rb1	363:365	arg1	effect					341:346	the osteogenesis and angiogenesis effect	307:346	the osteogenesis and angiogenesis effect of Ginsenoside Rb1	307:365	However, there is little study on the osteogenesis and angiogenesis effect of Ginsenoside Rb1.					
35153297	0	51	theme	silk/micro-nano	49:63	arg1	scaffolds					106:114	silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds	49:114	silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect	49:135	The osteogenesis of Ginsenoside Rb1 incorporated silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect.					
35153297	9	52	theme	Ginsenoside	1392:1402	arg1	Rb1					1404:1406	Ginsenoside Rb1	1392:1406	Ginsenoside Rb1	1392:1406	All the outcomes hint that Ginsenoside Rb1 could reinforce the osteogenesis differentiation and angiogenesis factor's expression of BMSCs.					
35153297	5	53	from	effect	806:811	arg1	HAp/silk					853:860	micro-nano HAp/silk	842:860	micro-nano HAp/silk in rat calvarial defect models	842:891	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	2	54	theme	Ginsenoside	351:361	arg1	Rb1					363:365	Ginsenoside Rb1	351:365	Ginsenoside Rb1	351:365	However, there is little study on the osteogenesis and angiogenesis effect of Ginsenoside Rb1.					
35153297	4	55	theme	Rb1	530:532	arg1	role					510:513	the role	506:513	the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs	506:632	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	5	56	theme	micro-nano	664:673	arg1	granules					679:686	micro-nano HAp granules	664:686	micro-nano HAp granules combined with silk	664:705	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	10	57	theme	Ginsenoside	1575:1585	arg1	Rb1					1587:1589	Ginsenoside Rb1	1575:1589	Ginsenoside Rb1 to repair bone defect	1575:1611	Moreover, micro-nano HAp combined with silk could act as a carrier for Ginsenoside Rb1 to repair bone defect.					
35153297	5	58	theme	HAp	675:677	arg1	granules					679:686	micro-nano HAp granules	664:686	micro-nano HAp granules combined with silk	664:705	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	1	59	theme	effective	159:167	arg1	Rb1					150:152	Ginsenoside Rb1	138:152	Ginsenoside Rb1	138:152	Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system.					
35153297	1	59	theme	effective	159:167	arg1	constituent					169:179	the effective constituent	155:179	the effective constituent of ginseng	155:190	Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system.					
35153297	4	60	theme	genes	590:594	arg1	expression					596:605	osteogenic genes expression	579:605	osteogenic genes expression	579:605	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	4	60	theme	genes	590:594	arg1	viability					542:550	cell viability	537:550	cell viability	537:550	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	0	61	theme	composite	96:104	arg1	scaffolds					106:114	silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds	49:114	silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect	49:135	The osteogenesis of Ginsenoside Rb1 incorporated silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect.					
35153297	4	62	theme	rat	624:626	arg1	BMSCs					628:632	rat BMSCs	624:632	rat BMSCs	624:632	In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly.					
35153297	5	63	from	HAp/silk	853:860	arg1	models					886:891	rat calvarial defect models	865:891	rat calvarial defect models	865:891	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	6	64	theme	angiogenic	1099:1108	arg1	viability					1035:1043	cell viability	1030:1043	cell viability	1030:1043	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	6	64	theme	angiogenic	1099:1108	arg1	expression					1116:1125	osteogenic and angiogenic genes expression	1084:1125	osteogenic and angiogenic genes expression	1084:1125	It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression.					
35153297	1	65	theme	immunity	256:263	arg1	system					265:270	the immunity system	252:270	the immunity system	252:270	Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system.					
35153297	0	66	theme	alginate	87:94	arg1	scaffolds					106:114	silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds	49:114	silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect	49:135	The osteogenesis of Ginsenoside Rb1 incorporated silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect.					
35153297	3	67	from	system	406:411	arg1	defect					467:472	bone defect	462:472	bone defect	462:472	Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive.					
35153297	5	68	theme	Rb1	767:769	arg1	system					745:750	a delivery system	734:750	a delivery system of Ginsenoside Rb1	734:769	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	5	69	theme	rat	865:867	arg1	models					886:891	rat calvarial defect models	865:891	rat calvarial defect models	865:891	Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively.					
35153297	7	70	theme	Ginsenoside	1239:1249	arg1	Rb1					1251:1253	Ginsenoside Rb1	1239:1253	Ginsenoside Rb1	1239:1253	Meanwhile, micro-nano HAp granules combined with silk were fabricated smoothly and were a delivery carrier for Ginsenoside Rb1.					
35153297	9	71	theme	osteogenesis	1428:1439	arg1	differentiation					1441:1455	osteogenesis differentiation	1428:1455	osteogenesis differentiation	1428:1455	All the outcomes hint that Ginsenoside Rb1 could reinforce the osteogenesis differentiation and angiogenesis factor's expression of BMSCs.					
37217043	4	0	theme	suitable	685:692	arg1	system					694:699	a suitable system	683:699	a suitable system for the envisaged application	683:729	The variables considered critical to obtain a suitable system for the envisaged application are the composition of hydrogels, especially the concentration of chitosan, and the concentration ratio between chitosan and collagen.					
37217043	6	1	theme	mechanical	1055:1064	arg1	properties					1066:1075	scaffold mechanical properties	1046:1075	scaffold mechanical properties	1046:1075	To assess the influence of the above-mentioned variables on scaffold mechanical properties, a Design of Experiments (DoE) approach was exploited, which resulted in the identification of the best hydrogel composition.					
37217043	2	2	theme	numerous	344:351	arg1	cues					362:365	numerous positive cues	344:365	numerous positive cues for wound repair	344:382	It is made of collagen, the most abundant protein of mammals ECM, and chitosan, a natural polysaccharide endowed with numerous positive cues for wound repair.					
37217043	4	3	theme	chitosan	797:804	arg1	variables					643:651	The variables	639:651	The variables considered critical to obtain a suitable system for the envisaged application	639:729	The variables considered critical to obtain a suitable system for the envisaged application are the composition of hydrogels, especially the concentration of chitosan, and the concentration ratio between chitosan and collagen.					
37217043	4	3	theme	chitosan	797:804	arg1	ratio					829:833	the concentration ratio	811:833	the concentration ratio between chitosan and collagen	811:863	The variables considered critical to obtain a suitable system for the envisaged application are the composition of hydrogels, especially the concentration of chitosan, and the concentration ratio between chitosan and collagen.					
37217043	4	3	theme	chitosan	797:804	arg1	composition					739:749	the composition	735:749	the composition of hydrogels	735:762	The variables considered critical to obtain a suitable system for the envisaged application are the composition of hydrogels, especially the concentration of chitosan, and the concentration ratio between chitosan and collagen.					
37217043	4	3	theme	chitosan	797:804	arg1	concentration					780:792	especially the concentration	765:792	especially the concentration of chitosan	765:804	The variables considered critical to obtain a suitable system for the envisaged application are the composition of hydrogels, especially the concentration of chitosan, and the concentration ratio between chitosan and collagen.					
37217043	6	4	from	influence	1000:1008	arg1	properties					1066:1075	scaffold mechanical properties	1046:1075	scaffold mechanical properties	1046:1075	To assess the influence of the above-mentioned variables on scaffold mechanical properties, a Design of Experiments (DoE) approach was exploited, which resulted in the identification of the best hydrogel composition.					
37217043	7	5	from	assays	1224:1229	arg1	model					1279:1283	a murine model	1270:1283	a murine model	1270:1283	In vitro and in vivo assays on a fibroblast model cell line and on a murine model, respectively, demonstrated scaffold biocompatibility, biomimicry, and safety.					
37217043	7	5	from	assays	1224:1229	arg1	line					1258:1261	a fibroblast model cell line	1234:1261	a fibroblast model cell line	1234:1261	In vitro and in vivo assays on a fibroblast model cell line and on a murine model, respectively, demonstrated scaffold biocompatibility, biomimicry, and safety.					
37217043	6	6	theme	Experiments	1090:1100	arg1	approach					1108:1115	Experiments (DoE) approach	1090:1115	Experiments (DoE) approach	1090:1115	To assess the influence of the above-mentioned variables on scaffold mechanical properties, a Design of Experiments (DoE) approach was exploited, which resulted in the identification of the best hydrogel composition.					
37217043	3	7	theme	cross-linking	497:509	arg1	agent					511:515	cross-linking agent	497:515	cross-linking agent	497:515	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	5	8	theme	high	899:902	arg1	porosity					904:911	high porosity	899:911	high porosity	899:911	Stable systems, characterized by high porosity and stability, were obtained thanks to the use of freeze-drying process.					
37217043	3	9	with	hydrogel	578:585	arg1	structure					628:636	a highly interconnected 3D internal structure	592:636	a highly interconnected 3D internal structure	592:636	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	5	10	theme	freeze-drying	963:975	arg1	process					977:983	freeze-drying process	963:983	freeze-drying process	963:983	Stable systems, characterized by high porosity and stability, were obtained thanks to the use of freeze-drying process.					
37217043	2	11	theme	mammals	279:285	arg1	protein					268:274	the most abundant protein	250:274	the most abundant protein of mammals ECM	250:289	It is made of collagen, the most abundant protein of mammals ECM, and chitosan, a natural polysaccharide endowed with numerous positive cues for wound repair.					
37217043	6	12	theme	approach	1108:1115	arg1	Design					1080:1085	a Design	1078:1085	a Design of Experiments (DoE) approach	1078:1115	To assess the influence of the above-mentioned variables on scaffold mechanical properties, a Design of Experiments (DoE) approach was exploited, which resulted in the identification of the best hydrogel composition.					
37217043	7	13	dep	in	1216:1217	arg1	vivo					1219:1222	vivo	1219:1222	vivo	1219:1222	In vitro and in vivo assays on a fibroblast model cell line and on a murine model, respectively, demonstrated scaffold biocompatibility, biomimicry, and safety.					
37217043	4	14	theme	envisaged	709:717	arg1	application					719:729	the envisaged application	705:729	the envisaged application	705:729	The variables considered critical to obtain a suitable system for the envisaged application are the composition of hydrogels, especially the concentration of chitosan, and the concentration ratio between chitosan and collagen.					
37217043	3	15	theme	glucose	461:467	arg1	addition					449:456	the addition	445:456	the addition of glucose	445:467	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	3	15	theme	glucose	461:467	arg1	addition					470:477	addition	470:477	addition of tannic acid as cross-linking agent	470:515	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	3	15	theme	glucose	461:467	arg1	ultrasonication					521:535	ultrasonication	521:535	ultrasonication	521:535	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	5	16	theme	process	977:983	arg1	use					956:958	the use	952:958	the use of freeze-drying process	952:983	Stable systems, characterized by high porosity and stability, were obtained thanks to the use of freeze-drying process.					
37217043	5	17	theme	Stable	866:871	arg1	systems					873:879	Stable systems	866:879	Stable systems	866:879	Stable systems, characterized by high porosity and stability, were obtained thanks to the use of freeze-drying process.					
37217043	2	18	theme	protein	268:274	arg1	ECM					287:289	the most abundant protein of mammals ECM	250:289	the most abundant protein of mammals ECM	250:289	It is made of collagen, the most abundant protein of mammals ECM, and chitosan, a natural polysaccharide endowed with numerous positive cues for wound repair.					
37217043	3	19	dep	methods	409:415	arg1	irradiation					428:438	UV irradiation	425:438	UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication	425:535	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	7	20	theme	fibroblast	1236:1245	arg1	line					1258:1261	a fibroblast model cell line	1234:1261	a fibroblast model cell line	1234:1261	In vitro and in vivo assays on a fibroblast model cell line and on a murine model, respectively, demonstrated scaffold biocompatibility, biomimicry, and safety.					
37217043	7	21	theme	cell	1253:1256	arg1	line					1258:1261	a fibroblast model cell line	1234:1261	a fibroblast model cell line	1234:1261	In vitro and in vivo assays on a fibroblast model cell line and on a murine model, respectively, demonstrated scaffold biocompatibility, biomimicry, and safety.					
37217043	2	22	theme	wound	371:375	arg1	repair					377:382	wound repair	371:382	wound repair	371:382	It is made of collagen, the most abundant protein of mammals ECM, and chitosan, a natural polysaccharide endowed with numerous positive cues for wound repair.					
37217043	0	23	theme	Formulation	0:10	arg1	development					12:22	Formulation development	0:22	Formulation development of collagen/chitosan-based porous	0:56	Formulation development of collagen/chitosan-based porous scaffolds for skin wounds repair and regeneration.					
37217043	1	24	theme	chronic	205:211	arg1	ulcers					218:223	chronic skin ulcers	205:223	chronic skin ulcers	205:223	Herein we developed a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers.					
37217043	0	25	theme	collagen/chitosan-based	27:49	arg1	porous					51:56	collagen/chitosan-based porous	27:56	collagen/chitosan-based porous	27:56	Formulation development of collagen/chitosan-based porous scaffolds for skin wounds repair and regeneration.					
37217043	7	26	theme	scaffold	1313:1320	arg1	biocompatibility					1322:1337	scaffold biocompatibility	1313:1337	scaffold biocompatibility	1313:1337	In vitro and in vivo assays on a fibroblast model cell line and on a murine model, respectively, demonstrated scaffold biocompatibility, biomimicry, and safety.					
37217043	4	27	theme	hydrogels	754:762	arg1	variables					643:651	The variables	639:651	The variables considered critical to obtain a suitable system for the envisaged application	639:729	The variables considered critical to obtain a suitable system for the envisaged application are the composition of hydrogels, especially the concentration of chitosan, and the concentration ratio between chitosan and collagen.					
37217043	4	27	theme	hydrogels	754:762	arg1	ratio					829:833	the concentration ratio	811:833	the concentration ratio between chitosan and collagen	811:863	The variables considered critical to obtain a suitable system for the envisaged application are the composition of hydrogels, especially the concentration of chitosan, and the concentration ratio between chitosan and collagen.					
37217043	4	27	theme	hydrogels	754:762	arg1	composition					739:749	the composition	735:749	the composition of hydrogels	735:762	The variables considered critical to obtain a suitable system for the envisaged application are the composition of hydrogels, especially the concentration of chitosan, and the concentration ratio between chitosan and collagen.					
37217043	4	27	theme	hydrogels	754:762	arg1	concentration					780:792	especially the concentration	765:792	especially the concentration of chitosan	765:804	The variables considered critical to obtain a suitable system for the envisaged application are the composition of hydrogels, especially the concentration of chitosan, and the concentration ratio between chitosan and collagen.					
37217043	1	28	theme	skin	213:216	arg1	ulcers					218:223	chronic skin ulcers	205:223	chronic skin ulcers	205:223	Herein we developed a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers.					
37217043	6	29	theme	best	1176:1179	arg1	composition					1190:1200	the best hydrogel composition	1172:1200	the best hydrogel composition	1172:1200	To assess the influence of the above-mentioned variables on scaffold mechanical properties, a Design of Experiments (DoE) approach was exploited, which resulted in the identification of the best hydrogel composition.					
37217043	0	30	dep	wounds	77:82	arg1	wounds					77:82	skin wounds repair and regeneration	72:106	skin wounds repair and regeneration	72:106	Formulation development of collagen/chitosan-based porous scaffolds for skin wounds repair and regeneration.					
37217043	0	30	dep	wounds	77:82	arg1	regeneration					95:106	regeneration	95:106	regeneration	95:106	Formulation development of collagen/chitosan-based porous scaffolds for skin wounds repair and regeneration.					
37217043	0	30	dep	wounds	77:82	arg1	repair					84:89	repair	84:89	repair	84:89	Formulation development of collagen/chitosan-based porous scaffolds for skin wounds repair and regeneration.					
37217043	1	31	theme	ulcers	218:223	arg1	treatment					192:200	the treatment	188:200	the treatment of chronic skin ulcers	188:223	Herein we developed a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers.					
37217043	3	32	link	cross-linked	565:576	arg1	hydrogel					578:585	a cross-linked hydrogel	563:585	a cross-linked hydrogel with a highly interconnected 3D internal structure	563:636	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	3	33	theme	tannic	482:487	arg1	acid					489:492	tannic acid	482:492	tannic acid	482:492	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	1	34	link	cross-linked	153:164	arg1	scaffold					166:173	a hydrogel based porous cross-linked scaffold	129:173	a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers	129:223	Herein we developed a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers.					
37217043	4	35	theme	concentration	815:827	arg1	ratio					829:833	the concentration ratio	811:833	the concentration ratio between chitosan and collagen	811:863	The variables considered critical to obtain a suitable system for the envisaged application are the composition of hydrogels, especially the concentration of chitosan, and the concentration ratio between chitosan and collagen.					
37217043	3	36	theme	UV	425:426	arg1	irradiation					428:438	UV irradiation	425:438	UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication	425:535	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	6	37	theme	scaffold	1046:1053	arg1	properties					1066:1075	scaffold mechanical properties	1046:1075	scaffold mechanical properties	1046:1075	To assess the influence of the above-mentioned variables on scaffold mechanical properties, a Design of Experiments (DoE) approach was exploited, which resulted in the identification of the best hydrogel composition.					
37217043	0	38	theme	porous	51:56	arg1	development					12:22	Formulation development	0:22	Formulation development of collagen/chitosan-based porous	0:56	Formulation development of collagen/chitosan-based porous scaffolds for skin wounds repair and regeneration.					
37217043	2	39	with	endowed	331:337	arg1	cues					362:365	numerous positive cues	344:365	numerous positive cues for wound repair	344:382	It is made of collagen, the most abundant protein of mammals ECM, and chitosan, a natural polysaccharide endowed with numerous positive cues for wound repair.					
37217043	0	40	theme	skin	72:75	arg1	wounds					77:82	skin wounds repair and regeneration	72:106	skin wounds repair and regeneration	72:106	Formulation development of collagen/chitosan-based porous scaffolds for skin wounds repair and regeneration.					
37217043	0	40	theme	skin	72:75	arg1	regeneration					95:106	regeneration	95:106	regeneration	95:106	Formulation development of collagen/chitosan-based porous scaffolds for skin wounds repair and regeneration.					
37217043	0	40	theme	skin	72:75	arg1	repair					84:89	repair	84:89	repair	84:89	Formulation development of collagen/chitosan-based porous scaffolds for skin wounds repair and regeneration.					
37217043	3	41	theme	acid	489:492	arg1	addition					449:456	the addition	445:456	the addition of glucose	445:467	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	3	41	theme	acid	489:492	arg1	addition					470:477	addition	470:477	addition of tannic acid as cross-linking agent	470:515	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	3	41	theme	acid	489:492	arg1	ultrasonication					521:535	ultrasonication	521:535	ultrasonication	521:535	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	3	42	theme	cross-linked	565:576	arg1	hydrogel					578:585	a cross-linked hydrogel	563:585	a cross-linked hydrogel with a highly interconnected 3D internal structure	563:636	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	2	43	theme	endowed	331:337	arg1	polysaccharide					316:329	a natural polysaccharide	306:329	a natural polysaccharide endowed with numerous positive cues for wound repair	306:382	It is made of collagen, the most abundant protein of mammals ECM, and chitosan, a natural polysaccharide endowed with numerous positive cues for wound repair.					
37217043	2	43	theme	endowed	331:337	arg1	chitosan					296:303	chitosan	296:303	chitosan	296:303	It is made of collagen, the most abundant protein of mammals ECM, and chitosan, a natural polysaccharide endowed with numerous positive cues for wound repair.					
37217043	3	44	theme	cross-linking	395:407	arg1	methods					409:415	Different cross-linking methods	385:415	Different cross-linking methods	385:415	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	1	45	theme	hydrogel	131:138	arg1	scaffold					166:173	a hydrogel based porous cross-linked scaffold	129:173	a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers	129:223	Herein we developed a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers.					
37217043	6	46	theme	hydrogel	1181:1188	arg1	composition					1190:1200	the best hydrogel composition	1172:1200	the best hydrogel composition	1172:1200	To assess the influence of the above-mentioned variables on scaffold mechanical properties, a Design of Experiments (DoE) approach was exploited, which resulted in the identification of the best hydrogel composition.					
37217043	3	47	with	irradiation	428:438	arg1	addition					449:456	the addition	445:456	the addition of glucose	445:467	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	3	47	with	irradiation	428:438	arg1	ultrasonication					521:535	ultrasonication	521:535	ultrasonication	521:535	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	3	47	with	irradiation	428:438	arg1	addition					470:477	addition	470:477	addition of tannic acid as cross-linking agent	470:515	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	3	48	theme	3D	616:617	arg1	structure					628:636	a highly interconnected 3D internal structure	592:636	a highly interconnected 3D internal structure	592:636	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	2	49	theme	abundant	259:266	arg1	protein					268:274	the most abundant protein	250:274	the most abundant protein of mammals ECM	250:289	It is made of collagen, the most abundant protein of mammals ECM, and chitosan, a natural polysaccharide endowed with numerous positive cues for wound repair.					
37217043	6	50	theme	above-mentioned	1017:1031	arg1	variables					1033:1041	the above-mentioned variables	1013:1041	the above-mentioned variables	1013:1041	To assess the influence of the above-mentioned variables on scaffold mechanical properties, a Design of Experiments (DoE) approach was exploited, which resulted in the identification of the best hydrogel composition.					
37217043	1	51	theme	based	140:144	arg1	scaffold					166:173	a hydrogel based porous cross-linked scaffold	129:173	a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers	129:223	Herein we developed a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers.					
37217043	6	52	theme	variables	1033:1041	arg1	influence					1000:1008	the influence	996:1008	the influence of the above-mentioned variables on scaffold mechanical properties	996:1075	To assess the influence of the above-mentioned variables on scaffold mechanical properties, a Design of Experiments (DoE) approach was exploited, which resulted in the identification of the best hydrogel composition.					
37217043	7	53	dep	In	1203:1204	arg1	vitro					1206:1210	vitro	1206:1210	vitro	1206:1210	In vitro and in vivo assays on a fibroblast model cell line and on a murine model, respectively, demonstrated scaffold biocompatibility, biomimicry, and safety.					
37217043	3	54	theme	internal	619:626	arg1	structure					628:636	a highly interconnected 3D internal structure	592:636	a highly interconnected 3D internal structure	592:636	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	1	55	theme	porous	146:151	arg1	scaffold					166:173	a hydrogel based porous cross-linked scaffold	129:173	a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers	129:223	Herein we developed a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers.					
37217043	2	56	theme	natural	308:314	arg1	polysaccharide					316:329	a natural polysaccharide	306:329	a natural polysaccharide endowed with numerous positive cues for wound repair	306:382	It is made of collagen, the most abundant protein of mammals ECM, and chitosan, a natural polysaccharide endowed with numerous positive cues for wound repair.					
37217043	2	56	theme	natural	308:314	arg1	chitosan					296:303	chitosan	296:303	chitosan	296:303	It is made of collagen, the most abundant protein of mammals ECM, and chitosan, a natural polysaccharide endowed with numerous positive cues for wound repair.					
37217043	7	57	theme	in	1216:1217	arg1	assays					1224:1229	In vitro and in vivo assays	1203:1229	In vitro and in vivo assays on a fibroblast model cell line and on a murine model	1203:1283	In vitro and in vivo assays on a fibroblast model cell line and on a murine model, respectively, demonstrated scaffold biocompatibility, biomimicry, and safety.					
37217043	6	58	theme	composition	1190:1200	arg1	identification					1154:1167	the identification	1150:1167	the identification of the best hydrogel composition	1150:1200	To assess the influence of the above-mentioned variables on scaffold mechanical properties, a Design of Experiments (DoE) approach was exploited, which resulted in the identification of the best hydrogel composition.					
37217043	7	59	theme	murine	1272:1277	arg1	model					1279:1283	a murine model	1270:1283	a murine model	1270:1283	In vitro and in vivo assays on a fibroblast model cell line and on a murine model, respectively, demonstrated scaffold biocompatibility, biomimicry, and safety.					
37217043	1	60	theme	cross-linked	153:164	arg1	scaffold					166:173	a hydrogel based porous cross-linked scaffold	129:173	a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers	129:223	Herein we developed a hydrogel based porous cross-linked scaffold intended for the treatment of chronic skin ulcers.					
37217043	3	61	theme	Different	385:393	arg1	methods					409:415	Different cross-linking methods	385:415	Different cross-linking methods	385:415	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	3	62	theme	interconnected	601:614	arg1	structure					628:636	a highly interconnected 3D internal structure	592:636	a highly interconnected 3D internal structure	592:636	Different cross-linking methods, namely UV irradiation with the addition of glucose, addition of tannic acid as cross-linking agent and ultrasonication, were employed to prepare a cross-linked hydrogel with a highly interconnected 3D internal structure.					
37217043	7	63	theme	model	1247:1251	arg1	line					1258:1261	a fibroblast model cell line	1234:1261	a fibroblast model cell line	1234:1261	In vitro and in vivo assays on a fibroblast model cell line and on a murine model, respectively, demonstrated scaffold biocompatibility, biomimicry, and safety.					
37217043	2	64	theme	positive	353:360	arg1	cues					362:365	numerous positive cues	344:365	numerous positive cues for wound repair	344:382	It is made of collagen, the most abundant protein of mammals ECM, and chitosan, a natural polysaccharide endowed with numerous positive cues for wound repair.					
37217043	7	65	theme	In	1203:1204	arg1	assays					1224:1229	In vitro and in vivo assays	1203:1229	In vitro and in vivo assays on a fibroblast model cell line and on a murine model	1203:1283	In vitro and in vivo assays on a fibroblast model cell line and on a murine model, respectively, demonstrated scaffold biocompatibility, biomimicry, and safety.					
36289259	0	0	theme	action	81:86	arg1	mechanism					68:76	mechanism	68:76	mechanism of action	68:86	Hetastarch induced volume changes of starving adenoma and suggested mechanism of action.					
36289259	5	1	theme	HCT116	595:600	arg1	cells					602:606	HCT116 cells	595:606	HCT116 cells	595:606	The significant volume increase of HCT116 cells was observed, but only for starving cell culture, suggesting that the starving is essential for the neoplasms-specific volume change.					
36289259	4	2	theme	same	532:535	arg1	solution					541:548	the same EMR solution	528:548	the same EMR solution	528:548	The HCT116 cell line of human colon cancer was exposed to the same EMR solution in vitro.					
36289259	4	3	theme	cell	481:484	arg1	line					486:489	The HCT116 cell line	470:489	The HCT116 cell line of human colon cancer	470:511	The HCT116 cell line of human colon cancer was exposed to the same EMR solution in vitro.					
36289259	5	4	theme	cells	602:606	arg1	increase					583:590	The significant volume increase	560:590	The significant volume increase of HCT116 cells	560:606	The significant volume increase of HCT116 cells was observed, but only for starving cell culture, suggesting that the starving is essential for the neoplasms-specific volume change.					
36289259	1	5	dep	step	128:131	arg1	EMR					170:172	EMR	170:172	EMR	170:172	Submucosal injection is often required step during endoscopic mucosal resection (EMR).					
36289259	1	5	dep	step	128:131	arg1	resection					159:167	endoscopic mucosal resection	140:167	endoscopic mucosal resection (EMR)	140:173	Submucosal injection is often required step during endoscopic mucosal resection (EMR).					
36289259	2	6	contain	containing	246:255	arg2	hetastarch					257:266	hetastarch	257:266	hetastarch (HES)	257:272	In clinical practice we have observed that the EMR injection solution containing hetastarch (HES) lead to selective increase of the neoplasms volume, facilitating their resection.					
36289259	2	6	contain	containing	246:255	arg1	solution					237:244	the EMR injection solution	219:244	the EMR injection solution containing hetastarch (HES)	219:272	In clinical practice we have observed that the EMR injection solution containing hetastarch (HES) lead to selective increase of the neoplasms volume, facilitating their resection.					
36289259	2	6	contain	containing	246:255	arg2	HES					269:271	HES	269:271	HES	269:271	In clinical practice we have observed that the EMR injection solution containing hetastarch (HES) lead to selective increase of the neoplasms volume, facilitating their resection.					
36289259	6	7	theme	HES	835:837	arg1	crucial					852:858	crucial	852:858	crucial	852:858	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	6	7	theme	HES	835:837	arg1	component					839:847	the HES component	831:847	the HES component	831:847	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	4	8	theme	EMR	537:539	arg1	solution					541:548	the same EMR solution	528:548	the same EMR solution	528:548	The HCT116 cell line of human colon cancer was exposed to the same EMR solution in vitro.					
36289259	5	9	theme	significant	564:574	arg1	increase					583:590	The significant volume increase	560:590	The significant volume increase of HCT116 cells	560:606	The significant volume increase of HCT116 cells was observed, but only for starving cell culture, suggesting that the starving is essential for the neoplasms-specific volume change.					
36289259	6	10	theme	hydrolysis	896:905	arg1	subject					874:880	subject	874:880	subject	874:880	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	6	11	theme	solution	822:829	arg1	composition					779:789	the iso-oncotic composition	763:789	the iso-oncotic composition of the EMR submucosa injection solution	763:829	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	5	12	theme	volume	576:581	arg1	increase					583:590	The significant volume increase	560:590	The significant volume increase of HCT116 cells	560:606	The significant volume increase of HCT116 cells was observed, but only for starving cell culture, suggesting that the starving is essential for the neoplasms-specific volume change.					
36289259	6	13	theme	injection	812:820	arg1	solution					822:829	the EMR submucosa injection solution	794:829	the EMR submucosa injection solution	794:829	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	6	14	theme	neoplastic	998:1007	arg1	tissue					1009:1014	the neoplastic tissue	994:1014	the neoplastic tissue	994:1014	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	5	15	dep	observed	612:619	arg1	starving					635:642	starving	635:642	starving cell culture	635:655	The significant volume increase of HCT116 cells was observed, but only for starving cell culture, suggesting that the starving is essential for the neoplasms-specific volume change.					
36289259	6	16	theme	starch	889:894	arg1	hydrolysis					896:905	the starch hydrolysis	885:905	the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue	885:1014	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	1	17	theme	Submucosal	89:98	arg1	injection					100:108	Submucosal injection	89:108	Submucosal injection	89:108	Submucosal injection is often required step during endoscopic mucosal resection (EMR).					
36289259	6	18	theme	facilitated	919:929	arg1	transport					931:939	facilitated transport	919:939	facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue	919:1014	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	3	19	theme	possible	397:404	arg1	mechanisms					406:415	the possible mechanisms	393:415	the possible mechanisms of such behaviour	393:433	The aim of this study was to explore the possible mechanisms of such behaviour, which was not reported elsewhere.					
36289259	4	20	theme	cancer	506:511	arg1	line					486:489	The HCT116 cell line	470:489	The HCT116 cell line of human colon cancer	470:511	The HCT116 cell line of human colon cancer was exposed to the same EMR solution in vitro.					
36289259	6	21	from	submucosa	979:987	arg1	transport					931:939	facilitated transport	919:939	facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue	919:1014	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	6	21	from	submucosa	979:987	arg1	monosaccharides					954:968	resulting monosaccharides	944:968	resulting monosaccharides from the submucosa	944:987	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	2	22	theme	selective	282:290	arg1	increase					292:299	selective increase	282:299	selective increase of the neoplasms volume	282:323	In clinical practice we have observed that the EMR injection solution containing hetastarch (HES) lead to selective increase of the neoplasms volume, facilitating their resection.					
36289259	0	23	theme	volume	19:24	arg1	changes					26:32	volume changes	19:32	volume changes of starving adenoma	19:52	Hetastarch induced volume changes of starving adenoma and suggested mechanism of action.					
36289259	6	24	from	transport	931:939	arg1	submucosa					979:987	the submucosa	975:987	the submucosa	975:987	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	5	25	theme	cell	644:647	arg1	culture					649:655	cell culture	644:655	cell culture	644:655	The significant volume increase of HCT116 cells was observed, but only for starving cell culture, suggesting that the starving is essential for the neoplasms-specific volume change.					
36289259	6	26	theme	submucosa	802:810	arg1	solution					822:829	the EMR submucosa injection solution	794:829	the EMR submucosa injection solution	794:829	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	0	27	theme	starving	37:44	arg1	adenoma					46:52	starving adenoma	37:52	starving adenoma	37:52	Hetastarch induced volume changes of starving adenoma and suggested mechanism of action.					
36289259	6	28	theme	EMR	798:800	arg1	submucosa					802:810	the EMR submucosa	794:810	the EMR submucosa injection solution	794:829	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	2	29	theme	EMR	223:225	arg1	solution					237:244	the EMR injection solution	219:244	the EMR injection solution containing hetastarch (HES)	219:272	In clinical practice we have observed that the EMR injection solution containing hetastarch (HES) lead to selective increase of the neoplasms volume, facilitating their resection.					
36289259	3	30	theme	such	420:423	arg1	behaviour					425:433	such behaviour	420:433	such behaviour	420:433	The aim of this study was to explore the possible mechanisms of such behaviour, which was not reported elsewhere.					
36289259	5	31	theme	volume	727:732	arg1	change					734:739	the neoplasms-specific volume change	704:739	the neoplasms-specific volume change	704:739	The significant volume increase of HCT116 cells was observed, but only for starving cell culture, suggesting that the starving is essential for the neoplasms-specific volume change.					
36289259	3	32	theme	behaviour	425:433	arg1	mechanisms					406:415	the possible mechanisms	393:415	the possible mechanisms of such behaviour	393:433	The aim of this study was to explore the possible mechanisms of such behaviour, which was not reported elsewhere.					
36289259	3	33	theme	study	372:376	arg1	aim					360:362	The aim	356:362	The aim of this study	356:376	The aim of this study was to explore the possible mechanisms of such behaviour, which was not reported elsewhere.					
36289259	0	34	theme	adenoma	46:52	arg1	changes					26:32	volume changes	19:32	volume changes of starving adenoma	19:52	Hetastarch induced volume changes of starving adenoma and suggested mechanism of action.					
36289259	4	35	theme	colon	500:504	arg1	cancer					506:511	human colon cancer	494:511	human colon cancer	494:511	The HCT116 cell line of human colon cancer was exposed to the same EMR solution in vitro.					
36289259	2	36	theme	volume	318:323	arg1	increase					292:299	selective increase	282:299	selective increase of the neoplasms volume	282:323	In clinical practice we have observed that the EMR injection solution containing hetastarch (HES) lead to selective increase of the neoplasms volume, facilitating their resection.					
36289259	4	37	theme	human	494:498	arg1	cancer					506:511	human colon cancer	494:511	human colon cancer	494:511	The HCT116 cell line of human colon cancer was exposed to the same EMR solution in vitro.					
36289259	6	38	theme	iso-oncotic	767:777	arg1	composition					779:789	the iso-oncotic composition	763:789	the iso-oncotic composition of the EMR submucosa injection solution	763:829	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	2	39	theme	neoplasms	308:316	arg1	volume					318:323	the neoplasms volume	304:323	the neoplasms volume	304:323	In clinical practice we have observed that the EMR injection solution containing hetastarch (HES) lead to selective increase of the neoplasms volume, facilitating their resection.					
36289259	6	40	theme	monosaccharides	954:968	arg1	transport					931:939	facilitated transport	919:939	facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue	919:1014	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	5	41	theme	neoplasms-specific	708:725	arg1	change					734:739	the neoplasms-specific volume change	704:739	the neoplasms-specific volume change	704:739	The significant volume increase of HCT116 cells was observed, but only for starving cell culture, suggesting that the starving is essential for the neoplasms-specific volume change.					
36289259	6	42	theme	resulting	944:952	arg1	monosaccharides					954:968	resulting monosaccharides	944:968	resulting monosaccharides from the submucosa	944:987	We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.					
36289259	2	43	theme	clinical	179:186	arg1	practice					188:195	clinical practice	179:195	clinical practice	179:195	In clinical practice we have observed that the EMR injection solution containing hetastarch (HES) lead to selective increase of the neoplasms volume, facilitating their resection.					
36289259	1	44	theme	endoscopic	140:149	arg1	EMR					170:172	EMR	170:172	EMR	170:172	Submucosal injection is often required step during endoscopic mucosal resection (EMR).					
36289259	1	44	theme	endoscopic	140:149	arg1	resection					159:167	endoscopic mucosal resection	140:167	endoscopic mucosal resection (EMR)	140:173	Submucosal injection is often required step during endoscopic mucosal resection (EMR).					
36289259	2	45	theme	injection	227:235	arg1	solution					237:244	the EMR injection solution	219:244	the EMR injection solution containing hetastarch (HES)	219:272	In clinical practice we have observed that the EMR injection solution containing hetastarch (HES) lead to selective increase of the neoplasms volume, facilitating their resection.					
36289259	1	46	theme	mucosal	151:157	arg1	EMR					170:172	EMR	170:172	EMR	170:172	Submucosal injection is often required step during endoscopic mucosal resection (EMR).					
36289259	1	46	theme	mucosal	151:157	arg1	resection					159:167	endoscopic mucosal resection	140:167	endoscopic mucosal resection (EMR)	140:173	Submucosal injection is often required step during endoscopic mucosal resection (EMR).					
36257763	0	0	theme	automated	60:68	arg1	extraction					89:98	the fully automated dispersive pipette extraction	50:98	the fully automated dispersive pipette extraction	50:98	MIL-88B(Fe)/cellulose microspheres as sorbent for the fully automated dispersive pipette extraction towards trace sulfonamides in milk samples prior to UPLC-MS/MS analysis.					
36257763	1	1	theme	TGA	271:273	arg1	means					252:256	the means	248:256	the means of SEM, XRD, TGA and N2 adsorption-desorption test	248:307	MIL-88B(Fe)/cellulose microspheres (MIL-88B(Fe)/CMs) were characterized by the means of SEM, XRD, TGA and N2 adsorption-desorption test.					
36257763	3	2	theme	milk	591:594	arg1	samples					596:602	milk samples	591:602	milk samples	591:602	Coupled to UPLC-MS/MS, the proposed method was employed for the analysis of trace sulfonamides (SAs) in milk samples.					
36257763	5	3	dep	sensitivity	885:895	arg1	limit					898:902	limit	898:902	limit of detection: 0.00660-0.0136 μg kg-1	898:939	Under the optimal conditions, good linearity (r ≥ 0.9978 for five analytes), high sensitivity (limit of detection: 0.00660-0.0136 μg kg-1) and satisfactory recovery (69.8%-100.9%) were achieved.					
36257763	2	4	theme	MIL-88B	455:461	arg1	loss					447:450	the loss	443:450	the loss of MIL-88B(Fe) in DPX	443:472	The composite was used as the sorbent for fully automated dispersive pipette extraction (DPX), after introducing CMs as the support, the loss of MIL-88B(Fe) in DPX was avoided.					
36257763	3	5	theme	trace	563:567	arg1	SAs					583:585	SAs	583:585	SAs	583:585	Coupled to UPLC-MS/MS, the proposed method was employed for the analysis of trace sulfonamides (SAs) in milk samples.					
36257763	3	5	theme	trace	563:567	arg1	sulfonamides					569:580	trace sulfonamides	563:580	trace sulfonamides (SAs) in milk samples	563:602	Coupled to UPLC-MS/MS, the proposed method was employed for the analysis of trace sulfonamides (SAs) in milk samples.					
36257763	0	6	theme	pipette	81:87	arg1	extraction					89:98	the fully automated dispersive pipette extraction	50:98	the fully automated dispersive pipette extraction	50:98	MIL-88B(Fe)/cellulose microspheres as sorbent for the fully automated dispersive pipette extraction towards trace sulfonamides in milk samples prior to UPLC-MS/MS analysis.					
36257763	1	7	theme	N2	279:280	arg1	test					304:307	N2 adsorption-desorption test	279:307	N2 adsorption-desorption test	279:307	MIL-88B(Fe)/cellulose microspheres (MIL-88B(Fe)/CMs) were characterized by the means of SEM, XRD, TGA and N2 adsorption-desorption test.					
36257763	4	8	theme	extraction	634:643	arg1	efficiency					645:654	the extraction efficiency	630:654	the extraction efficiency	630:654	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	0	9	theme	dispersive	70:79	arg1	extraction					89:98	the fully automated dispersive pipette extraction	50:98	the fully automated dispersive pipette extraction	50:98	MIL-88B(Fe)/cellulose microspheres as sorbent for the fully automated dispersive pipette extraction towards trace sulfonamides in milk samples prior to UPLC-MS/MS analysis.					
36257763	1	10	theme	adsorption-desorption	282:302	arg1	test					304:307	N2 adsorption-desorption test	279:307	N2 adsorption-desorption test	279:307	MIL-88B(Fe)/cellulose microspheres (MIL-88B(Fe)/CMs) were characterized by the means of SEM, XRD, TGA and N2 adsorption-desorption test.					
36257763	5	11	theme	optimal	813:819	arg1	conditions					821:830	the optimal conditions	809:830	the optimal conditions	809:830	Under the optimal conditions, good linearity (r ≥ 0.9978 for five analytes), high sensitivity (limit of detection: 0.00660-0.0136 μg kg-1) and satisfactory recovery (69.8%-100.9%) were achieved.					
36257763	7	12	theme	friendliness	1238:1249	arg1	advantages					1166:1175	advantages	1166:1175	advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation	1166:1264	Compared with other methods for the pretreatment of SAs, the proposed method showed advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation, providing a promising protocol for sample preparation.					
36257763	1	13	theme	test	304:307	arg1	means					252:256	the means	248:256	the means of SEM, XRD, TGA and N2 adsorption-desorption test	248:307	MIL-88B(Fe)/cellulose microspheres (MIL-88B(Fe)/CMs) were characterized by the means of SEM, XRD, TGA and N2 adsorption-desorption test.					
36257763	4	14	theme	aspiration	702:711	arg1	aspiration					702:711	aspiration	702:711	aspiration	702:711	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	14	theme	aspiration	702:711	arg1	solvent					779:785	elution solvent	771:785	elution solvent	771:785	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	14	theme	aspiration	702:711	arg1	adsorbent					741:749	adsorbent	741:749	adsorbent	741:749	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	14	theme	aspiration	702:711	arg1	dispense					717:724	dispense	717:724	dispense	717:724	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	14	theme	aspiration	702:711	arg1	solution					680:687	sample solution	673:687	sample solution	673:687	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	14	theme	aspiration	702:711	arg1	type					752:755	type	752:755	type	752:755	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	14	theme	aspiration	702:711	arg1	rate					694:697	the rate	690:697	the rate of aspiration and dispense	690:724	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	14	theme	aspiration	702:711	arg1	amount					727:732	amount	727:732	amount of the adsorbent	727:749	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	14	theme	aspiration	702:711	arg1	pH					667:668	pH	667:668	pH of sample solution	667:687	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	14	theme	aspiration	702:711	arg1	volume					761:766	volume	761:766	volume of elution solvent	761:785	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	7	15	theme	automation	1255:1264	arg1	advantages					1166:1175	advantages	1166:1175	advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation	1166:1264	Compared with other methods for the pretreatment of SAs, the proposed method showed advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation, providing a promising protocol for sample preparation.					
36257763	7	16	theme	promising	1279:1287	arg1	protocol					1289:1296	a promising protocol	1277:1296	a promising protocol for sample preparation	1277:1319	Compared with other methods for the pretreatment of SAs, the proposed method showed advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation, providing a promising protocol for sample preparation.					
36257763	4	17	theme	solvent	779:785	arg1	aspiration					702:711	aspiration	702:711	aspiration	702:711	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	17	theme	solvent	779:785	arg1	solvent					779:785	elution solvent	771:785	elution solvent	771:785	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	17	theme	solvent	779:785	arg1	adsorbent					741:749	adsorbent	741:749	adsorbent	741:749	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	17	theme	solvent	779:785	arg1	dispense					717:724	dispense	717:724	dispense	717:724	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	17	theme	solvent	779:785	arg1	solution					680:687	sample solution	673:687	sample solution	673:687	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	17	theme	solvent	779:785	arg1	type					752:755	type	752:755	type	752:755	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	17	theme	solvent	779:785	arg1	rate					694:697	the rate	690:697	the rate of aspiration and dispense	690:724	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	17	theme	solvent	779:785	arg1	amount					727:732	amount	727:732	amount of the adsorbent	727:749	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	17	theme	solvent	779:785	arg1	pH					667:668	pH	667:668	pH of sample solution	667:687	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	17	theme	solvent	779:785	arg1	volume					761:766	volume	761:766	volume of elution solvent	761:785	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	5	18	theme	satisfactory	946:957	arg1	%					980:980	69.8%-100.9%	969:980	69.8%-100.9%	969:980	Under the optimal conditions, good linearity (r ≥ 0.9978 for five analytes), high sensitivity (limit of detection: 0.00660-0.0136 μg kg-1) and satisfactory recovery (69.8%-100.9%) were achieved.					
36257763	5	18	theme	satisfactory	946:957	arg1	recovery					959:966	satisfactory recovery	946:966	satisfactory recovery (69.8%-100.9%)	946:981	Under the optimal conditions, good linearity (r ≥ 0.9978 for five analytes), high sensitivity (limit of detection: 0.00660-0.0136 μg kg-1) and satisfactory recovery (69.8%-100.9%) were achieved.					
36257763	4	19	theme	dispense	717:724	arg1	aspiration					702:711	aspiration	702:711	aspiration	702:711	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	19	theme	dispense	717:724	arg1	solvent					779:785	elution solvent	771:785	elution solvent	771:785	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	19	theme	dispense	717:724	arg1	adsorbent					741:749	adsorbent	741:749	adsorbent	741:749	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	19	theme	dispense	717:724	arg1	dispense					717:724	dispense	717:724	dispense	717:724	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	19	theme	dispense	717:724	arg1	solution					680:687	sample solution	673:687	sample solution	673:687	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	19	theme	dispense	717:724	arg1	type					752:755	type	752:755	type	752:755	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	19	theme	dispense	717:724	arg1	rate					694:697	the rate	690:697	the rate of aspiration and dispense	690:724	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	19	theme	dispense	717:724	arg1	amount					727:732	amount	727:732	amount of the adsorbent	727:749	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	19	theme	dispense	717:724	arg1	pH					667:668	pH	667:668	pH of sample solution	667:687	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	19	theme	dispense	717:724	arg1	volume					761:766	volume	761:766	volume of elution solvent	761:785	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	3	20	from	analysis	551:558	arg1	samples					596:602	milk samples	591:602	milk samples	591:602	Coupled to UPLC-MS/MS, the proposed method was employed for the analysis of trace sulfonamides (SAs) in milk samples.					
36257763	7	21	theme	sample	1302:1307	arg1	preparation					1309:1319	sample preparation	1302:1319	sample preparation	1302:1319	Compared with other methods for the pretreatment of SAs, the proposed method showed advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation, providing a promising protocol for sample preparation.					
36257763	2	22	theme	pipette	379:385	arg1	DPX					399:401	DPX	399:401	DPX	399:401	The composite was used as the sorbent for fully automated dispersive pipette extraction (DPX), after introducing CMs as the support, the loss of MIL-88B(Fe) in DPX was avoided.					
36257763	2	22	theme	pipette	379:385	arg1	extraction					387:396	fully automated dispersive pipette extraction	352:396	fully automated dispersive pipette extraction (DPX)	352:402	The composite was used as the sorbent for fully automated dispersive pipette extraction (DPX), after introducing CMs as the support, the loss of MIL-88B(Fe) in DPX was avoided.					
36257763	7	23	theme	high	1180:1183	arg1	sensitivity					1185:1195	high sensitivity	1180:1195	high sensitivity	1180:1195	Compared with other methods for the pretreatment of SAs, the proposed method showed advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation, providing a promising protocol for sample preparation.					
36257763	7	24	theme	proposed	1143:1150	arg1	method					1152:1157	the proposed method	1139:1157	the proposed method	1139:1157	Compared with other methods for the pretreatment of SAs, the proposed method showed advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation, providing a promising protocol for sample preparation.					
36257763	2	25	theme	dispersive	368:377	arg1	DPX					399:401	DPX	399:401	DPX	399:401	The composite was used as the sorbent for fully automated dispersive pipette extraction (DPX), after introducing CMs as the support, the loss of MIL-88B(Fe) in DPX was avoided.					
36257763	2	25	theme	dispersive	368:377	arg1	extraction					387:396	fully automated dispersive pipette extraction	352:396	fully automated dispersive pipette extraction (DPX)	352:402	The composite was used as the sorbent for fully automated dispersive pipette extraction (DPX), after introducing CMs as the support, the loss of MIL-88B(Fe) in DPX was avoided.					
36257763	7	26	theme	sorbent	1203:1209	arg1	consumption					1211:1221	less sorbent consumption	1198:1221	less sorbent consumption	1198:1221	Compared with other methods for the pretreatment of SAs, the proposed method showed advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation, providing a promising protocol for sample preparation.					
36257763	7	27	theme	SAs	1134:1136	arg1	pretreatment					1118:1129	the pretreatment	1114:1129	the pretreatment of SAs	1114:1136	Compared with other methods for the pretreatment of SAs, the proposed method showed advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation, providing a promising protocol for sample preparation.					
36257763	2	28	theme	automated	358:366	arg1	DPX					399:401	DPX	399:401	DPX	399:401	The composite was used as the sorbent for fully automated dispersive pipette extraction (DPX), after introducing CMs as the support, the loss of MIL-88B(Fe) in DPX was avoided.					
36257763	2	28	theme	automated	358:366	arg1	extraction					387:396	fully automated dispersive pipette extraction	352:396	fully automated dispersive pipette extraction (DPX)	352:402	The composite was used as the sorbent for fully automated dispersive pipette extraction (DPX), after introducing CMs as the support, the loss of MIL-88B(Fe) in DPX was avoided.					
36257763	0	29	theme	trace	108:112	arg1	sulfonamides					114:125	trace sulfonamides	108:125	trace sulfonamides in milk samples prior to UPLC-MS/MS analysis	108:170	MIL-88B(Fe)/cellulose microspheres as sorbent for the fully automated dispersive pipette extraction towards trace sulfonamides in milk samples prior to UPLC-MS/MS analysis.					
36257763	4	30	theme	elution	771:777	arg1	solvent					779:785	elution solvent	771:785	elution solvent	771:785	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	7	31	theme	environmental	1224:1236	arg1	friendliness					1238:1249	environmental friendliness	1224:1249	environmental friendliness	1224:1249	Compared with other methods for the pretreatment of SAs, the proposed method showed advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation, providing a promising protocol for sample preparation.					
36257763	7	32	theme	sensitivity	1185:1195	arg1	advantages					1166:1175	advantages	1166:1175	advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation	1166:1264	Compared with other methods for the pretreatment of SAs, the proposed method showed advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation, providing a promising protocol for sample preparation.					
36257763	5	33	theme	%	973:973	arg1	%					980:980	69.8%-100.9%	969:980	69.8%-100.9%	969:980	Under the optimal conditions, good linearity (r ≥ 0.9978 for five analytes), high sensitivity (limit of detection: 0.00660-0.0136 μg kg-1) and satisfactory recovery (69.8%-100.9%) were achieved.					
36257763	5	33	theme	%	973:973	arg1	recovery					959:966	satisfactory recovery	946:966	satisfactory recovery (69.8%-100.9%)	946:981	Under the optimal conditions, good linearity (r ≥ 0.9978 for five analytes), high sensitivity (limit of detection: 0.00660-0.0136 μg kg-1) and satisfactory recovery (69.8%-100.9%) were achieved.					
36257763	0	34	theme	milk	130:133	arg1	samples					135:141	milk samples	130:141	milk samples prior to UPLC-MS/MS analysis	130:170	MIL-88B(Fe)/cellulose microspheres as sorbent for the fully automated dispersive pipette extraction towards trace sulfonamides in milk samples prior to UPLC-MS/MS analysis.					
36257763	6	35	theme	extraction	1063:1072	arg1	cycles					1074:1079	eight extraction cycles	1057:1079	eight extraction cycles	1057:1079	Furthermore, the sorbent showed desirable reusability over eight extraction cycles.					
36257763	1	36	theme	/cellulose	184:193	arg1	/CMs					220:223	MIL-88B(Fe)/CMs	209:223	MIL-88B(Fe)/CMs	209:223	MIL-88B(Fe)/cellulose microspheres (MIL-88B(Fe)/CMs) were characterized by the means of SEM, XRD, TGA and N2 adsorption-desorption test.					
36257763	1	36	theme	/cellulose	184:193	arg1	microspheres					195:206	MIL-88B(Fe)/cellulose microspheres	173:206	MIL-88B(Fe)/cellulose microspheres (MIL-88B(Fe)/CMs)	173:224	MIL-88B(Fe)/cellulose microspheres (MIL-88B(Fe)/CMs) were characterized by the means of SEM, XRD, TGA and N2 adsorption-desorption test.					
36257763	0	37	theme	/cellulose	11:20	arg1	microspheres					22:33	MIL-88B(Fe)/cellulose microspheres	0:33	MIL-88B(Fe)/cellulose microspheres as sorbent for the fully automated dispersive pipette extraction towards trace sulfonamides in milk samples prior to UPLC-MS/MS analysis.	0:171	MIL-88B(Fe)/cellulose microspheres as sorbent for the fully automated dispersive pipette extraction towards trace sulfonamides in milk samples prior to UPLC-MS/MS analysis.					
36257763	3	38	from	sulfonamides	569:580	arg1	samples					596:602	milk samples	591:602	milk samples	591:602	Coupled to UPLC-MS/MS, the proposed method was employed for the analysis of trace sulfonamides (SAs) in milk samples.					
36257763	3	39	theme	proposed	514:521	arg1	method					523:528	the proposed method	510:528	the proposed method	510:528	Coupled to UPLC-MS/MS, the proposed method was employed for the analysis of trace sulfonamides (SAs) in milk samples.					
36257763	4	40	theme	solution	680:687	arg1	aspiration					702:711	aspiration	702:711	aspiration	702:711	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	40	theme	solution	680:687	arg1	solvent					779:785	elution solvent	771:785	elution solvent	771:785	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	40	theme	solution	680:687	arg1	adsorbent					741:749	adsorbent	741:749	adsorbent	741:749	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	40	theme	solution	680:687	arg1	dispense					717:724	dispense	717:724	dispense	717:724	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	40	theme	solution	680:687	arg1	solution					680:687	sample solution	673:687	sample solution	673:687	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	40	theme	solution	680:687	arg1	type					752:755	type	752:755	type	752:755	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	40	theme	solution	680:687	arg1	rate					694:697	the rate	690:697	the rate of aspiration and dispense	690:724	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	40	theme	solution	680:687	arg1	amount					727:732	amount	727:732	amount of the adsorbent	727:749	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	40	theme	solution	680:687	arg1	pH					667:668	pH	667:668	pH of sample solution	667:687	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	40	theme	solution	680:687	arg1	volume					761:766	volume	761:766	volume of elution solvent	761:785	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	5	41	dep	linearity	838:846	arg1	r ≥ 0.9978					849:858	r ≥ 0.9978	849:858	r ≥ 0.9978 for five analytes	849:876	Under the optimal conditions, good linearity (r ≥ 0.9978 for five analytes), high sensitivity (limit of detection: 0.00660-0.0136 μg kg-1) and satisfactory recovery (69.8%-100.9%) were achieved.					
36257763	1	42	theme	SEM	261:263	arg1	means					252:256	the means	248:256	the means of SEM, XRD, TGA and N2 adsorption-desorption test	248:307	MIL-88B(Fe)/cellulose microspheres (MIL-88B(Fe)/CMs) were characterized by the means of SEM, XRD, TGA and N2 adsorption-desorption test.					
36257763	3	43	theme	sulfonamides	569:580	arg1	analysis					551:558	the analysis	547:558	the analysis of trace sulfonamides (SAs) in milk samples	547:602	Coupled to UPLC-MS/MS, the proposed method was employed for the analysis of trace sulfonamides (SAs) in milk samples.					
36257763	4	44	theme	adsorbent	741:749	arg1	aspiration					702:711	aspiration	702:711	aspiration	702:711	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	44	theme	adsorbent	741:749	arg1	solvent					779:785	elution solvent	771:785	elution solvent	771:785	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	44	theme	adsorbent	741:749	arg1	adsorbent					741:749	adsorbent	741:749	adsorbent	741:749	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	44	theme	adsorbent	741:749	arg1	dispense					717:724	dispense	717:724	dispense	717:724	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	44	theme	adsorbent	741:749	arg1	solution					680:687	sample solution	673:687	sample solution	673:687	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	44	theme	adsorbent	741:749	arg1	type					752:755	type	752:755	type	752:755	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	44	theme	adsorbent	741:749	arg1	rate					694:697	the rate	690:697	the rate of aspiration and dispense	690:724	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	44	theme	adsorbent	741:749	arg1	amount					727:732	amount	727:732	amount of the adsorbent	727:749	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	44	theme	adsorbent	741:749	arg1	pH					667:668	pH	667:668	pH of sample solution	667:687	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	4	44	theme	adsorbent	741:749	arg1	volume					761:766	volume	761:766	volume of elution solvent	761:785	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	5	45	theme	detection	907:915	arg1	limit					898:902	limit	898:902	limit of detection: 0.00660-0.0136 μg kg-1	898:939	Under the optimal conditions, good linearity (r ≥ 0.9978 for five analytes), high sensitivity (limit of detection: 0.00660-0.0136 μg kg-1) and satisfactory recovery (69.8%-100.9%) were achieved.					
36257763	1	46	theme	XRD	266:268	arg1	means					252:256	the means	248:256	the means of SEM, XRD, TGA and N2 adsorption-desorption test	248:307	MIL-88B(Fe)/cellulose microspheres (MIL-88B(Fe)/CMs) were characterized by the means of SEM, XRD, TGA and N2 adsorption-desorption test.					
36257763	0	47	theme	UPLC-MS/MS	152:161	arg1	analysis					163:170	UPLC-MS/MS analysis	152:170	UPLC-MS/MS analysis	152:170	MIL-88B(Fe)/cellulose microspheres as sorbent for the fully automated dispersive pipette extraction towards trace sulfonamides in milk samples prior to UPLC-MS/MS analysis.					
36257763	5	48	theme	high	880:883	arg1	sensitivity					885:895	high sensitivity	880:895	high sensitivity (limit of detection: 0.00660-0.0136 μg kg-1)	880:940	Under the optimal conditions, good linearity (r ≥ 0.9978 for five analytes), high sensitivity (limit of detection: 0.00660-0.0136 μg kg-1) and satisfactory recovery (69.8%-100.9%) were achieved.					
36257763	5	49	theme	good	833:836	arg1	linearity					838:846	good linearity	833:846	good linearity (r ≥ 0.9978 for five analytes)	833:877	Under the optimal conditions, good linearity (r ≥ 0.9978 for five analytes), high sensitivity (limit of detection: 0.00660-0.0136 μg kg-1) and satisfactory recovery (69.8%-100.9%) were achieved.					
36257763	7	50	theme	consumption	1211:1221	arg1	advantages					1166:1175	advantages	1166:1175	advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation	1166:1264	Compared with other methods for the pretreatment of SAs, the proposed method showed advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation, providing a promising protocol for sample preparation.					
36257763	7	51	theme	other	1096:1100	arg1	methods					1102:1108	other methods	1096:1108	other methods for the pretreatment of SAs	1096:1136	Compared with other methods for the pretreatment of SAs, the proposed method showed advantages of high sensitivity, less sorbent consumption, environmental friendliness and automation, providing a promising protocol for sample preparation.					
36257763	5	52	dep	limit	898:902	arg1	0.00660-0.0136 μg kg-1					918:939	0.00660-0.0136 μg kg-1	918:939	limit of detection: 0.00660-0.0136 μg kg-1	898:939	Under the optimal conditions, good linearity (r ≥ 0.9978 for five analytes), high sensitivity (limit of detection: 0.00660-0.0136 μg kg-1) and satisfactory recovery (69.8%-100.9%) were achieved.					
36257763	2	53	from	loss	447:450	arg1	DPX					470:472	DPX	470:472	DPX	470:472	The composite was used as the sorbent for fully automated dispersive pipette extraction (DPX), after introducing CMs as the support, the loss of MIL-88B(Fe) in DPX was avoided.					
36257763	4	54	theme	sample	673:678	arg1	solution					680:687	sample solution	673:687	sample solution	673:687	The parameters affecting the extraction efficiency, including pH of sample solution, the rate of aspiration and dispense, amount of the adsorbent, type and volume of elution solvent were optimized.					
36257763	0	55	from	sulfonamides	114:125	arg1	samples					135:141	milk samples	130:141	milk samples prior to UPLC-MS/MS analysis	130:170	MIL-88B(Fe)/cellulose microspheres as sorbent for the fully automated dispersive pipette extraction towards trace sulfonamides in milk samples prior to UPLC-MS/MS analysis.					
36257763	3	56	from	samples	596:602	arg1	analysis					551:558	the analysis	547:558	the analysis of trace sulfonamides (SAs) in milk samples	547:602	Coupled to UPLC-MS/MS, the proposed method was employed for the analysis of trace sulfonamides (SAs) in milk samples.					
36257763	2	57	used	used	328:331	arg2	composite					314:322	composite	314:322	composite	314:322	The composite was used as the sorbent for fully automated dispersive pipette extraction (DPX), after introducing CMs as the support, the loss of MIL-88B(Fe) in DPX was avoided.					
36257763	2	57	used	used	328:331	arg2	sorbent					340:346	the sorbent	336:346	the sorbent for fully automated dispersive pipette extraction (DPX)	336:402	The composite was used as the sorbent for fully automated dispersive pipette extraction (DPX), after introducing CMs as the support, the loss of MIL-88B(Fe) in DPX was avoided.					
36257763	6	58	theme	desirable	1030:1038	arg1	reusability					1040:1050	desirable reusability	1030:1050	desirable reusability	1030:1050	Furthermore, the sorbent showed desirable reusability over eight extraction cycles.					
35940431	7	0	theme	acid	1512:1515	arg1	biosynthesis					1657:1668	steroid hormone biosynthesis	1641:1668	steroid hormone biosynthesis	1641:1668	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	0	theme	acid	1512:1515	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	0	theme	acid	1512:1515	arg1	metabolism					1629:1638	glycerolipid metabolism	1616:1638	glycerolipid metabolism	1616:1638	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	0	theme	acid	1512:1515	arg1	biosynthesis					1570:1581	steroid hormone biosynthesis	1554:1581	steroid hormone biosynthesis	1554:1581	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	0	theme	acid	1512:1515	arg1	biosynthesis					1689:1700	primary bile acid biosynthesis	1671:1700	primary bile acid biosynthesis	1671:1700	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	0	theme	acid	1512:1515	arg1	metabolism					1604:1613	glycerophospholipid metabolism	1584:1613	glycerophospholipid metabolism	1584:1613	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	0	theme	acid	1512:1515	arg1	biosynthesis					1540:1551	fatty acid biosynthesis	1529:1551	fatty acid biosynthesis	1529:1551	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	10	1	theme	GLP-Cr	2299:2304	arg1	intervention					2306:2317	GLP-Cr intervention	2299:2317	GLP-Cr intervention	2299:2317	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	7	2	theme	Liver	1367:1371	arg1	analysis					1386:1393	Liver metabolomics analysis	1367:1393	Liver metabolomics analysis	1367:1393	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	10	3	theme	mRNA	2137:2140	arg1	levels					2142:2147	hepatic mRNA levels	2129:2147	hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c)	2129:2280	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	0	4	theme	high-fat	177:184	arg1	diet					204:207	high-fat and high-fructose diet	177:207	high-fat and high-fructose diet	177:207	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.					
35940431	8	5	theme	GLP-Cr	1724:1729	arg1	intervention					1731:1742	GLP-Cr intervention	1724:1742	GLP-Cr intervention	1724:1742	Moreover, GLP-Cr intervention regulated liver mRNA levels of key genes associated with glucose and lipid metabolism.					
35940431	5	6	theme	microbial	1147:1155	arg1	phylotypes					1157:1166	some intestinal microbial phylotypes	1131:1166	some intestinal microbial phylotypes	1131:1166	Besides, 16S rRNA amplicon sequencing showed that GLP-Cr intervention evidently ameliorated intestinal microbiota dysbiosis by changing the proportions of some intestinal microbial phylotypes.					
35940431	4	7	theme	weight	835:840	arg1	exaltation					816:825	the excessive exaltation	802:825	the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD	802:973	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	11	8	theme	functional	2611:2620	arg1	component					2622:2630	a functional component	2609:2630	a functional component in the prevention of MetS	2609:2656	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	11	8	theme	functional	2611:2620	arg1	GLP-Cr					2584:2589	GLP-Cr	2584:2589	GLP-Cr	2584:2589	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	3	9	theme	high-fructose	679:691	arg1	HFHFD					699:703	HFHFD	699:703	HFHFD	699:703	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	3	9	theme	high-fructose	679:691	arg1	diet					693:696	a high-fat and high-fructose diet	664:696	diet	693:696	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	0	10	theme	high-fructose	190:202	arg1	diet					204:207	high-fat and high-fructose diet	177:207	high-fat and high-fructose diet	177:207	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.					
35940431	1	11	theme	important	266:274	arg1	resource					296:303	an important chromium supplement resource	263:303	an important chromium supplement resource in recent years	263:319	Organic chromium is of great interest and has become an important chromium supplement resource in recent years because of its low toxicity and easy absorption.					
35940431	7	12	theme	biomarkers	1473:1482	arg1	levels					1458:1463	the levels	1454:1463	the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on	1454:1711	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	10	13	theme	excessive	2333:2341	arg1	accumulation					2343:2354	the excessive accumulation	2329:2354	the excessive accumulation of free fatty acids in the liver	2329:2387	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	9	14	theme	glucose	1849:1855	arg1	type					1869:1872	glucose transporter type 4	1849:1874	glucose transporter type 4 (Glut4)	1849:1882	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	9	14	theme	glucose	1849:1855	arg1	Glut4					1877:1881	Glut4	1877:1881	Glut4	1877:1881	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	3	15	theme	high-fat	666:673	arg1	HFHFD					699:703	HFHFD	699:703	HFHFD	699:703	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	3	15	theme	high-fat	666:673	arg1	diet					693:696	a high-fat and high-fructose diet	664:696	diet	693:696	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	8	16	theme	mRNA	1760:1763	arg1	levels					1765:1770	liver mRNA levels	1754:1770	liver mRNA levels of key genes associated with glucose and lipid metabolism	1754:1828	Moreover, GLP-Cr intervention regulated liver mRNA levels of key genes associated with glucose and lipid metabolism.					
35940431	4	17	theme	excessive	806:814	arg1	exaltation					816:825	the excessive exaltation	802:825	the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD	802:973	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	9	18	theme	mRNA	1939:1942	arg1	levels					1944:1949	the mRNA levels	1935:1949	the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver	1935:2042	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	6	19	theme	network-based	1194:1206	arg1	analysis					1208:1215	correlation network-based analysis	1182:1215	correlation network-based analysis	1182:1215	In addition, correlation network-based analysis indicated that the key intestinal microbial phylotypes were closely related to biochemical parameters associated with MetS under GLP-Cr intervention.					
35940431	6	20	theme	intestinal	1240:1249	arg1	phylotypes					1261:1270	the key intestinal microbial phylotypes	1232:1270	the key intestinal microbial phylotypes	1232:1270	In addition, correlation network-based analysis indicated that the key intestinal microbial phylotypes were closely related to biochemical parameters associated with MetS under GLP-Cr intervention.					
35940431	6	20	theme	intestinal	1240:1249	arg1	related					1285:1291	related	1285:1291	related	1285:1291	In addition, correlation network-based analysis indicated that the key intestinal microbial phylotypes were closely related to biochemical parameters associated with MetS under GLP-Cr intervention.					
35940431	4	21	theme	blood	870:874	arg1	glucose					876:882	fasting blood glucose	862:882	fasting blood glucose	862:882	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	21	theme	blood	870:874	arg1	weight					835:840	body weight	830:840	body weight	830:840	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	0	22	theme	metabolic	110:118	arg1	syndromes					120:128	metabolic syndromes	110:128	metabolic syndromes	110:128	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.					
35940431	3	23	theme	study	540:544	arg1	purpose					524:530	The purpose	520:530	The purpose of this study	520:544	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	8	24	theme	genes	1779:1783	arg1	levels					1765:1770	liver mRNA levels	1754:1770	liver mRNA levels of key genes associated with glucose and lipid metabolism	1754:1828	Moreover, GLP-Cr intervention regulated liver mRNA levels of key genes associated with glucose and lipid metabolism.					
35940431	1	25	theme	Organic	210:216	arg1	chromium					218:225	Organic chromium	210:225	Organic chromium	210:225	Organic chromium is of great interest and has become an important chromium supplement resource in recent years because of its low toxicity and easy absorption.					
35940431	7	26	theme	bile	1679:1682	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	26	theme	bile	1679:1682	arg1	biosynthesis					1689:1700	primary bile acid biosynthesis	1671:1700	primary bile acid biosynthesis	1671:1700	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	10	27	theme	hepatic	2129:2135	arg1	levels					2142:2147	hepatic mRNA levels	2129:2147	hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c)	2129:2280	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	6	28	theme	correlation	1182:1192	arg1	analysis					1208:1215	correlation network-based analysis	1182:1215	correlation network-based analysis	1182:1215	In addition, correlation network-based analysis indicated that the key intestinal microbial phylotypes were closely related to biochemical parameters associated with MetS under GLP-Cr intervention.					
35940431	9	29	theme	carboxykinase	1974:1986	arg1	levels					1944:1949	the mRNA levels	1935:1949	the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver	1935:2042	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	5	30	theme	rRNA	989:992	arg1	sequencing					1003:1012	16S rRNA amplicon sequencing	985:1012	16S rRNA amplicon sequencing	985:1012	Besides, 16S rRNA amplicon sequencing showed that GLP-Cr intervention evidently ameliorated intestinal microbiota dysbiosis by changing the proportions of some intestinal microbial phylotypes.					
35940431	4	31	theme	glucose	843:849	arg1	tolerance					851:859	glucose tolerance	843:859	glucose tolerance	843:859	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	31	theme	glucose	843:849	arg1	weight					835:840	body weight	830:840	body weight	830:840	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	11	32	theme	hepatic	2551:2557	arg1	pathways					2569:2576	hepatic metabolic pathways	2551:2576	hepatic metabolic pathways	2551:2576	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	9	33	from	levels	1944:1949	arg1	liver					2038:2042	the liver	2034:2042	the liver	2034:2042	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	10	34	theme	carboxylase	2201:2211	arg1	levels					2142:2147	hepatic mRNA levels	2129:2147	hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c)	2129:2280	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	0	35	theme	microbiota	145:154	arg1	dysbiosis					156:164	intestinal microbiota dysbiosis	134:164	intestinal microbiota dysbiosis	134:164	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.					
35940431	7	36	theme	hormone	1649:1655	arg1	biosynthesis					1657:1668	steroid hormone biosynthesis	1641:1668	steroid hormone biosynthesis	1641:1668	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	36	theme	hormone	1649:1655	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	37	dep	and	1703:1705	arg1	so					1707:1708	so	1707:1708	so	1707:1708	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	0	38	theme	lucidum	57:63	arg1	polysaccharide					65:78	Ganoderma lucidum polysaccharide	47:78	Ganoderma lucidum polysaccharide	47:78	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.					
35940431	3	39	theme	GLP-Cr	591:596	arg1	effects					580:586	the beneficial effects	565:586	the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action	565:732	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	7	40	theme	glycerolipid	1616:1627	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	40	theme	glycerolipid	1616:1627	arg1	metabolism					1629:1638	glycerolipid metabolism	1616:1638	glycerolipid metabolism	1616:1638	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	2	41	theme	lucidum	477:483	arg1	polysaccharide					485:498	Ganoderma lucidum polysaccharide	467:498	Ganoderma lucidum polysaccharide	467:498	In our previous study, we synthesized a novel organic chromium [GLP-Cr] through the chelation of Ganoderma lucidum polysaccharide and chromium (III).					
35940431	11	42	from	component	2622:2630	arg1	prevention					2639:2648	the prevention	2635:2648	the prevention of MetS	2635:2656	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	10	43	theme	element	2244:2250	arg1	Srebp-1c					2272:2279	Srebp-1c	2272:2279	Srebp-1c	2272:2279	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	43	theme	element	2244:2250	arg1	protein-1c					2260:2269	sterol regulatory element binding protein-1c	2226:2269	sterol regulatory element binding protein-1c (Srebp-1c)	2226:2280	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	1	44	theme	easy	353:356	arg1	absorption					358:367	easy absorption	353:367	easy absorption	353:367	Organic chromium is of great interest and has become an important chromium supplement resource in recent years because of its low toxicity and easy absorption.					
35940431	4	45	theme	hepatic	902:908	arg1	cholesterol					916:926	hepatic total cholesterol	902:926	hepatic total cholesterol (TC)	902:931	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	45	theme	hepatic	902:908	arg1	weight					835:840	body weight	830:840	body weight	830:840	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	45	theme	hepatic	902:908	arg1	TC					929:930	TC	929:930	TC	929:930	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	11	46	theme	composition	2535:2545	arg1	regulation					2507:2516	the regulation	2503:2516	the regulation of gut microbial composition and hepatic metabolic pathways	2503:2576	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	10	47	theme	sterol	2226:2231	arg1	Srebp-1c					2272:2279	Srebp-1c	2272:2279	Srebp-1c	2272:2279	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	47	theme	sterol	2226:2231	arg1	protein-1c					2260:2269	sterol regulatory element binding protein-1c	2226:2269	sterol regulatory element binding protein-1c (Srebp-1c)	2226:2280	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	9	48	theme	mRNA	1835:1838	arg1	level					1840:1844	The mRNA level	1831:1844	The mRNA level of glucose transporter type 4 (Glut4)	1831:1882	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	11	49	theme	gut	2521:2523	arg1	composition					2535:2545	gut microbial composition	2521:2545	gut microbial composition	2521:2545	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	2	50	theme	previous	377:384	arg1	study					386:390	our previous study	373:390	our previous study	373:390	In our previous study, we synthesized a novel organic chromium [GLP-Cr] through the chelation of Ganoderma lucidum polysaccharide and chromium (III).					
35940431	0	51	theme	Organic	0:6	arg1	chromium					8:15	Organic chromium	0:15	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide	0:78	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.					
35940431	1	52	theme	chromium	276:283	arg1	resource					296:303	an important chromium supplement resource	263:303	an important chromium supplement resource in recent years	263:319	Organic chromium is of great interest and has become an important chromium supplement resource in recent years because of its low toxicity and easy absorption.					
35940431	2	53	theme	chromium	424:431	arg1	[GLP-Cr					433:439	a novel organic chromium [GLP-Cr]	408:440	a novel organic chromium [GLP-Cr]	408:440	In our previous study, we synthesized a novel organic chromium [GLP-Cr] through the chelation of Ganoderma lucidum polysaccharide and chromium (III).					
35940431	10	54	theme	protein-1c	2260:2269	arg1	levels					2142:2147	hepatic mRNA levels	2129:2147	hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c)	2129:2280	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	2	55	theme	novel	410:414	arg1	[GLP-Cr					433:439	a novel organic chromium [GLP-Cr]	408:440	a novel organic chromium [GLP-Cr]	408:440	In our previous study, we synthesized a novel organic chromium [GLP-Cr] through the chelation of Ganoderma lucidum polysaccharide and chromium (III).					
35940431	11	56	theme	hyperglycemia	2436:2448	arg1	related					2492:2498	related	2492:2498	related	2492:2498	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	11	56	theme	hyperglycemia	2436:2448	arg1	prevention					2422:2431	the prevention	2418:2431	the prevention of hyperglycemia and dyslipidemia by GLP-Cr	2418:2475	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	7	57	theme	steroid	1554:1560	arg1	biosynthesis					1570:1581	steroid hormone biosynthesis	1554:1581	steroid hormone biosynthesis	1554:1581	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	57	theme	steroid	1554:1560	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	9	58	theme	transporter	1857:1867	arg1	type					1869:1872	glucose transporter type 4	1849:1874	glucose transporter type 4 (Glut4)	1849:1882	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	9	58	theme	transporter	1857:1867	arg1	Glut4					1877:1881	Glut4	1877:1881	Glut4	1877:1881	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	3	59	theme	syndromes	630:638	arg1	improvement					605:615	the improvement	601:615	the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action	601:732	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	4	60	theme	oral	762:765	arg1	administration					767:780	oral administration	762:780	oral administration of GLP-Cr	762:790	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	3	61	from	effects	580:586	arg1	improvement					605:615	the improvement	601:615	the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action	601:732	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	1	62	theme	recent	308:313	arg1	years					315:319	recent years	308:319	recent years	308:319	Organic chromium is of great interest and has become an important chromium supplement resource in recent years because of its low toxicity and easy absorption.					
35940431	5	63	theme	microbiota	1079:1088	arg1	dysbiosis					1090:1098	intestinal microbiota dysbiosis	1068:1098	intestinal microbiota dysbiosis	1068:1098	Besides, 16S rRNA amplicon sequencing showed that GLP-Cr intervention evidently ameliorated intestinal microbiota dysbiosis by changing the proportions of some intestinal microbial phylotypes.					
35940431	4	64	theme	total	910:914	arg1	cholesterol					916:926	hepatic total cholesterol	902:926	hepatic total cholesterol (TC)	902:931	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	64	theme	total	910:914	arg1	weight					835:840	body weight	830:840	body weight	830:840	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	64	theme	total	910:914	arg1	TC					929:930	TC	929:930	TC	929:930	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	7	65	theme	fatty	1529:1533	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	65	theme	fatty	1529:1533	arg1	biosynthesis					1540:1551	fatty acid biosynthesis	1529:1551	fatty acid biosynthesis	1529:1551	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	10	66	theme	GLP-Cr	2085:2090	arg1	intervention					2092:2103	GLP-Cr intervention	2085:2103	GLP-Cr intervention	2085:2103	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	66	theme	GLP-Cr	2085:2090	arg1	Meanwhile					2074:2082	Meanwhile	2074:2082	Meanwhile	2074:2082	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	67	from	accumulation	2343:2354	arg1	liver					2383:2387	the liver	2379:2387	the liver	2379:2387	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	7	68	theme	alpha-linolenic	1496:1510	arg1	biosynthesis					1657:1668	steroid hormone biosynthesis	1641:1668	steroid hormone biosynthesis	1641:1668	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	68	theme	alpha-linolenic	1496:1510	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	68	theme	alpha-linolenic	1496:1510	arg1	metabolism					1629:1638	glycerolipid metabolism	1616:1638	glycerolipid metabolism	1616:1638	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	68	theme	alpha-linolenic	1496:1510	arg1	biosynthesis					1570:1581	steroid hormone biosynthesis	1554:1581	steroid hormone biosynthesis	1554:1581	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	68	theme	alpha-linolenic	1496:1510	arg1	biosynthesis					1689:1700	primary bile acid biosynthesis	1671:1700	primary bile acid biosynthesis	1671:1700	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	68	theme	alpha-linolenic	1496:1510	arg1	metabolism					1604:1613	glycerophospholipid metabolism	1584:1613	glycerophospholipid metabolism	1584:1613	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	68	theme	alpha-linolenic	1496:1510	arg1	biosynthesis					1540:1551	fatty acid biosynthesis	1529:1551	fatty acid biosynthesis	1529:1551	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	6	69	theme	microbial	1251:1259	arg1	phylotypes					1261:1270	the key intestinal microbial phylotypes	1232:1270	the key intestinal microbial phylotypes	1232:1270	In addition, correlation network-based analysis indicated that the key intestinal microbial phylotypes were closely related to biochemical parameters associated with MetS under GLP-Cr intervention.					
35940431	6	69	theme	microbial	1251:1259	arg1	related					1285:1291	related	1285:1291	related	1285:1291	In addition, correlation network-based analysis indicated that the key intestinal microbial phylotypes were closely related to biochemical parameters associated with MetS under GLP-Cr intervention.					
35940431	5	70	theme	intestinal	1136:1145	arg1	phylotypes					1157:1166	some intestinal microbial phylotypes	1131:1166	some intestinal microbial phylotypes	1131:1166	Besides, 16S rRNA amplicon sequencing showed that GLP-Cr intervention evidently ameliorated intestinal microbiota dysbiosis by changing the proportions of some intestinal microbial phylotypes.					
35940431	9	71	theme	GLP-Cr	1910:1915	arg1	intervention					1917:1928	GLP-Cr intervention	1910:1928	GLP-Cr intervention	1910:1928	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	3	72	from	improvement	605:615	arg1	mice					650:653	mice	650:653	mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action	650:732	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	6	73	theme	key	1236:1238	arg1	phylotypes					1261:1270	the key intestinal microbial phylotypes	1232:1270	the key intestinal microbial phylotypes	1232:1270	In addition, correlation network-based analysis indicated that the key intestinal microbial phylotypes were closely related to biochemical parameters associated with MetS under GLP-Cr intervention.					
35940431	6	73	theme	key	1236:1238	arg1	related					1285:1291	related	1285:1291	related	1285:1291	In addition, correlation network-based analysis indicated that the key intestinal microbial phylotypes were closely related to biochemical parameters associated with MetS under GLP-Cr intervention.					
35940431	5	74	theme	phylotypes	1157:1166	arg1	proportions					1116:1126	the proportions	1112:1126	the proportions of some intestinal microbial phylotypes	1112:1166	Besides, 16S rRNA amplicon sequencing showed that GLP-Cr intervention evidently ameliorated intestinal microbiota dysbiosis by changing the proportions of some intestinal microbial phylotypes.					
35940431	4	75	theme	body	830:833	arg1	glucose					876:882	fasting blood glucose	862:882	fasting blood glucose	862:882	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	75	theme	body	830:833	arg1	cholesterol					916:926	hepatic total cholesterol	902:926	hepatic total cholesterol (TC)	902:931	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	75	theme	body	830:833	arg1	tolerance					851:859	glucose tolerance	843:859	glucose tolerance	843:859	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	75	theme	body	830:833	arg1	levels					952:957	triglyceride (TG) levels	934:957	triglyceride (TG) levels caused by HFHFD	934:973	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	75	theme	body	830:833	arg1	levels					894:899	lipid levels	888:899	lipid levels	888:899	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	75	theme	body	830:833	arg1	weight					835:840	body weight	830:840	body weight	830:840	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	0	76	attach	derived	17:23	arg1	chelation					34:42	the chelation	30:42	the chelation of Ganoderma lucidum polysaccharide	30:78	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.					
35940431	0	76	attach	derived	17:23	arg2	chromium					8:15	Organic chromium	0:15	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide	0:78	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.					
35940431	10	77	theme	differentiation	2163:2177	arg1	Cd36					2183:2186	Cd36	2183:2186	Cd36	2183:2186	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	77	theme	differentiation	2163:2177	arg1	Acc1					2216:2219	Acc1	2216:2219	Acc1	2216:2219	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	77	theme	differentiation	2163:2177	arg1	cluster					2152:2158	cluster	2152:2158	cluster of differentiation 36 (Cd36)	2152:2187	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	77	theme	differentiation	2163:2177	arg1	protein-1c					2260:2269	sterol regulatory element binding protein-1c	2226:2269	sterol regulatory element binding protein-1c (Srebp-1c)	2226:2280	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	77	theme	differentiation	2163:2177	arg1	Srebp-1c					2272:2279	Srebp-1c	2272:2279	Srebp-1c	2272:2279	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	77	theme	differentiation	2163:2177	arg1	carboxylase					2201:2211	acetyl-CoA carboxylase 1	2190:2213	acetyl-CoA carboxylase 1 (Acc1)	2190:2220	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	1	78	from	resource	296:303	arg1	years					315:319	recent years	308:319	recent years	308:319	Organic chromium is of great interest and has become an important chromium supplement resource in recent years because of its low toxicity and easy absorption.					
35940431	4	79	theme	GLP-Cr	785:790	arg1	administration					767:780	oral administration	762:780	oral administration of GLP-Cr	762:790	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	10	80	theme	cluster	2152:2158	arg1	levels					2142:2147	hepatic mRNA levels	2129:2147	hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c)	2129:2280	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	8	81	theme	liver	1754:1758	arg1	levels					1765:1770	liver mRNA levels	1754:1770	liver mRNA levels of key genes associated with glucose and lipid metabolism	1754:1828	Moreover, GLP-Cr intervention regulated liver mRNA levels of key genes associated with glucose and lipid metabolism.					
35940431	7	82	theme	acid	1684:1687	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	82	theme	acid	1684:1687	arg1	biosynthesis					1689:1700	primary bile acid biosynthesis	1671:1700	primary bile acid biosynthesis	1671:1700	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	83	theme	GLP-Cr	1410:1415	arg1	intervention					1417:1428	GLP-Cr intervention	1410:1428	GLP-Cr intervention	1410:1428	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	84	theme	primary	1671:1677	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	84	theme	primary	1671:1677	arg1	biosynthesis					1689:1700	primary bile acid biosynthesis	1671:1700	primary bile acid biosynthesis	1671:1700	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	4	85	theme	fasting	862:868	arg1	glucose					876:882	fasting blood glucose	862:882	fasting blood glucose	862:882	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	85	theme	fasting	862:868	arg1	weight					835:840	body weight	830:840	body weight	830:840	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	11	86	theme	metabolic	2559:2567	arg1	pathways					2569:2576	hepatic metabolic pathways	2551:2576	hepatic metabolic pathways	2551:2576	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	9	87	theme	phosphoenolpyruvate	1954:1972	arg1	Pepck					1989:1993	Pepck	1989:1993	Pepck	1989:1993	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	9	87	theme	phosphoenolpyruvate	1954:1972	arg1	carboxykinase					1974:1986	phosphoenolpyruvate carboxykinase	1954:1986	phosphoenolpyruvate carboxykinase (Pepck)	1954:1994	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	6	88	theme	biochemical	1296:1306	arg1	parameters					1308:1317	biochemical parameters	1296:1317	biochemical parameters associated with MetS under GLP-Cr intervention	1296:1364	In addition, correlation network-based analysis indicated that the key intestinal microbial phylotypes were closely related to biochemical parameters associated with MetS under GLP-Cr intervention.					
35940431	5	89	theme	16S	985:987	arg1	sequencing					1003:1012	16S rRNA amplicon sequencing	985:1012	16S rRNA amplicon sequencing	985:1012	Besides, 16S rRNA amplicon sequencing showed that GLP-Cr intervention evidently ameliorated intestinal microbiota dysbiosis by changing the proportions of some intestinal microbial phylotypes.					
35940431	3	90	theme	beneficial	569:578	arg1	effects					580:586	the beneficial effects	565:586	the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action	565:732	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	8	91	theme	key	1775:1777	arg1	genes					1779:1783	key genes	1775:1783	key genes associated with glucose and lipid metabolism	1775:1828	Moreover, GLP-Cr intervention regulated liver mRNA levels of key genes associated with glucose and lipid metabolism.					
35940431	7	92	theme	metabolomics	1373:1384	arg1	analysis					1386:1393	Liver metabolomics analysis	1367:1393	Liver metabolomics analysis	1367:1393	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	11	93	theme	dyslipidemia	2454:2465	arg1	related					2492:2498	related	2492:2498	related	2492:2498	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	11	93	theme	dyslipidemia	2454:2465	arg1	prevention					2422:2431	the prevention	2418:2431	the prevention of hyperglycemia and dyslipidemia by GLP-Cr	2418:2475	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	3	94	theme	action	727:732	arg1	mechanism					714:722	its mechanism	710:722	its mechanism of action	710:732	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	3	94	theme	action	727:732	arg1	HFHFD					699:703	HFHFD	699:703	HFHFD	699:703	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	3	94	theme	action	727:732	arg1	diet					693:696	a high-fat and high-fructose diet	664:696	diet	693:696	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	5	95	theme	amplicon	994:1001	arg1	sequencing					1003:1012	16S rRNA amplicon sequencing	985:1012	16S rRNA amplicon sequencing	985:1012	Besides, 16S rRNA amplicon sequencing showed that GLP-Cr intervention evidently ameliorated intestinal microbiota dysbiosis by changing the proportions of some intestinal microbial phylotypes.					
35940431	7	96	theme	steroid	1641:1647	arg1	biosynthesis					1657:1668	steroid hormone biosynthesis	1641:1668	steroid hormone biosynthesis	1641:1668	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	96	theme	steroid	1641:1647	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	10	97	theme	fatty	2364:2368	arg1	acids					2370:2374	free fatty acids	2359:2374	free fatty acids	2359:2374	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	98	theme	acetyl-CoA	2190:2199	arg1	carboxylase					2201:2211	acetyl-CoA carboxylase 1	2190:2213	acetyl-CoA carboxylase 1 (Acc1)	2190:2220	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	98	theme	acetyl-CoA	2190:2199	arg1	Acc1					2216:2219	Acc1	2216:2219	Acc1	2216:2219	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	99	theme	acids	2370:2374	arg1	accumulation					2343:2354	the excessive accumulation	2329:2354	the excessive accumulation of free fatty acids in the liver	2329:2387	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	9	100	theme	glucose-6-phosphatase	2000:2020	arg1	levels					1944:1949	the mRNA levels	1935:1949	the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver	1935:2042	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	0	101	theme	polysaccharide	65:78	arg1	chelation					34:42	the chelation	30:42	the chelation of Ganoderma lucidum polysaccharide	30:78	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.					
35940431	8	102	theme	lipid	1813:1817	arg1	metabolism					1819:1828	lipid metabolism	1813:1828	lipid metabolism	1813:1828	Moreover, GLP-Cr intervention regulated liver mRNA levels of key genes associated with glucose and lipid metabolism.					
35940431	2	103	theme	polysaccharide	485:498	arg1	chelation					454:462	the chelation	450:462	the chelation of Ganoderma lucidum polysaccharide and chromium (III)	450:517	In our previous study, we synthesized a novel organic chromium [GLP-Cr] through the chelation of Ganoderma lucidum polysaccharide and chromium (III).					
35940431	1	104	theme	low	336:338	arg1	toxicity					340:347	low toxicity	336:347	low toxicity	336:347	Organic chromium is of great interest and has become an important chromium supplement resource in recent years because of its low toxicity and easy absorption.					
35940431	7	105	theme	glycerophospholipid	1584:1602	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	105	theme	glycerophospholipid	1584:1602	arg1	metabolism					1604:1613	glycerophospholipid metabolism	1584:1613	glycerophospholipid metabolism	1584:1613	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	2	106	theme	Ganoderma	467:475	arg1	lucidum					477:483	Ganoderma lucidum	467:483	Ganoderma lucidum polysaccharide	467:498	In our previous study, we synthesized a novel organic chromium [GLP-Cr] through the chelation of Ganoderma lucidum polysaccharide and chromium (III).					
35940431	10	107	theme	binding	2252:2258	arg1	Srebp-1c					2272:2279	Srebp-1c	2272:2279	Srebp-1c	2272:2279	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	107	theme	binding	2252:2258	arg1	protein-1c					2260:2269	sterol regulatory element binding protein-1c	2226:2269	sterol regulatory element binding protein-1c (Srebp-1c)	2226:2280	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	6	108	theme	GLP-Cr	1346:1351	arg1	intervention					1353:1364	GLP-Cr intervention	1346:1364	GLP-Cr intervention	1346:1364	In addition, correlation network-based analysis indicated that the key intestinal microbial phylotypes were closely related to biochemical parameters associated with MetS under GLP-Cr intervention.					
35940431	5	109	theme	intestinal	1068:1077	arg1	dysbiosis					1090:1098	intestinal microbiota dysbiosis	1068:1098	intestinal microbiota dysbiosis	1068:1098	Besides, 16S rRNA amplicon sequencing showed that GLP-Cr intervention evidently ameliorated intestinal microbiota dysbiosis by changing the proportions of some intestinal microbial phylotypes.					
35940431	10	110	theme	free	2359:2362	arg1	acids					2370:2374	free fatty acids	2359:2374	free fatty acids	2359:2374	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	4	111	theme	lipid	888:892	arg1	levels					894:899	lipid levels	888:899	lipid levels	888:899	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	111	theme	lipid	888:892	arg1	weight					835:840	body weight	830:840	body weight	830:840	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	11	112	theme	microbial	2525:2533	arg1	composition					2535:2545	gut microbial composition	2521:2545	gut microbial composition	2521:2545	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	10	113	theme	regulatory	2233:2242	arg1	Srebp-1c					2272:2279	Srebp-1c	2272:2279	Srebp-1c	2272:2279	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	10	113	theme	regulatory	2233:2242	arg1	protein-1c					2260:2269	sterol regulatory element binding protein-1c	2226:2269	sterol regulatory element binding protein-1c (Srebp-1c)	2226:2280	Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver.					
35940431	2	114	theme	chromium	504:511	arg1	chelation					454:462	the chelation	450:462	the chelation of Ganoderma lucidum polysaccharide and chromium (III)	450:517	In our previous study, we synthesized a novel organic chromium [GLP-Cr] through the chelation of Ganoderma lucidum polysaccharide and chromium (III).					
35940431	5	115	theme	GLP-Cr	1026:1031	arg1	intervention					1033:1044	GLP-Cr intervention	1026:1044	GLP-Cr intervention	1026:1044	Besides, 16S rRNA amplicon sequencing showed that GLP-Cr intervention evidently ameliorated intestinal microbiota dysbiosis by changing the proportions of some intestinal microbial phylotypes.					
35940431	0	116	theme	intestinal	134:143	arg1	dysbiosis					156:164	intestinal microbiota dysbiosis	134:164	intestinal microbiota dysbiosis	134:164	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.					
35940431	11	117	theme	MetS	2653:2656	arg1	prevention					2639:2648	the prevention	2635:2648	the prevention of MetS	2635:2656	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	11	118	theme	pathways	2569:2576	arg1	regulation					2507:2516	the regulation	2503:2516	the regulation of gut microbial composition and hepatic metabolic pathways	2503:2576	These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.					
35940431	1	119	theme	supplement	285:294	arg1	resource					296:303	an important chromium supplement resource	263:303	an important chromium supplement resource in recent years	263:319	Organic chromium is of great interest and has become an important chromium supplement resource in recent years because of its low toxicity and easy absorption.					
35940431	7	120	theme	on	1710:1711	arg1	levels					1458:1463	the levels	1454:1463	the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on	1454:1711	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	2	121	theme	organic	416:422	arg1	[GLP-Cr					433:439	a novel organic chromium [GLP-Cr]	408:440	a novel organic chromium [GLP-Cr]	408:440	In our previous study, we synthesized a novel organic chromium [GLP-Cr] through the chelation of Ganoderma lucidum polysaccharide and chromium (III).					
35940431	3	122	theme	metabolic	620:628	arg1	MetS					641:644	MetS	641:644	MetS	641:644	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	3	122	theme	metabolic	620:628	arg1	syndromes					630:638	metabolic syndromes	620:638	metabolic syndromes (MetS)	620:645	The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action.					
35940431	7	123	theme	hormone	1562:1568	arg1	biosynthesis					1570:1581	steroid hormone biosynthesis	1554:1581	steroid hormone biosynthesis	1554:1581	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	123	theme	hormone	1562:1568	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	9	124	theme	type	1869:1872	arg1	level					1840:1844	The mRNA level	1831:1844	The mRNA level of glucose transporter type 4 (Glut4)	1831:1882	The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased.					
35940431	0	125	theme	Ganoderma	47:55	arg1	lucidum					57:63	Ganoderma lucidum	47:63	Ganoderma lucidum polysaccharide	47:78	Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.					
35940431	4	126	theme	triglyceride	934:945	arg1	levels					952:957	triglyceride (TG) levels	934:957	triglyceride (TG) levels caused by HFHFD	934:973	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	4	126	theme	triglyceride	934:945	arg1	weight					835:840	body weight	830:840	body weight	830:840	The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD.					
35940431	7	127	theme	acid	1535:1538	arg1	metabolism					1517:1526	alpha-linolenic acid metabolism	1496:1526	alpha-linolenic acid metabolism	1496:1526	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	7	127	theme	acid	1535:1538	arg1	biosynthesis					1540:1551	fatty acid biosynthesis	1529:1551	fatty acid biosynthesis	1529:1551	Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on.					
35940431	1	128	theme	great	233:237	arg1	interest					239:246	great interest	233:246	great interest	233:246	Organic chromium is of great interest and has become an important chromium supplement resource in recent years because of its low toxicity and easy absorption.					
35780919	2	0	theme	scanning	465:472	arg1	FEG-SEM					495:501	FEG-SEM	495:501	FEG-SEM	495:501	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	2	0	theme	scanning	465:472	arg1	microscopy					483:492	field emission scanning electron microscopy	450:492	field emission scanning electron microscopy (FEG-SEM)	450:502	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	3	1	theme	average	656:662	arg1	weight					669:674	average body weight	656:674	average body weight of 150 ± 10 g	656:688	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	2	2	theme	x-ray	525:529	arg1	diffraction					531:541	x-ray diffraction	525:541	x-ray diffraction (XRD)	525:547	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	2	2	theme	x-ray	525:529	arg1	XRD					544:546	XRD	544:546	XRD	544:546	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	0	3	theme	fluoride	87:94	arg1	toxicity					75:82	the toxicity	71:82	the toxicity of fluoride	71:94	Potential of chitosan nanoparticle/fluoride nanocomposite for reducing the toxicity of fluoride an in-vivo study on the rat heart functions: Hematopoietic and immune systems.					
35780919	3	4	theme	Dawley	643:648	arg1	rats					650:653	twenty-eight male adult Sprague Dawley rats	611:653	twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g)	611:689	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	3	5	theme	twenty-eight	611:622	arg1	rats					650:653	twenty-eight male adult Sprague Dawley rats	611:653	twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g)	611:689	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	3	6	theme	well-balanced	934:946	arg1	diet					959:962	a well-balanced commercial diet	932:962	a well-balanced commercial diet	932:962	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	7	7	theme	modified	1372:1379	arg1	chitosan					1381:1388	The modified chitosan	1368:1388	The modified chitosan with fluoride	1368:1402	The modified chitosan with fluoride decreases the toxicity of fluoride via improving the rat heart function due to the presence of Cs NPs helped to mitigate some of the negative effects of fluoride therapy.					
35780919	7	8	theme	heart	1461:1465	arg1	function					1467:1474	the rat heart function	1453:1474	the rat heart function	1453:1474	The modified chitosan with fluoride decreases the toxicity of fluoride via improving the rat heart function due to the presence of Cs NPs helped to mitigate some of the negative effects of fluoride therapy.					
35780919	3	9	theme	commercial	948:957	arg1	diet					959:962	a well-balanced commercial diet	932:962	a well-balanced commercial diet	932:962	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	3	10	theme	12-h	907:910	arg1	photoperiod					912:922	a 12-h photoperiod	905:922	a 12-h photoperiod	905:922	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	0	11	theme	in-vivo	99:105	arg1	study					107:111	an in-vivo study	96:111	an in-vivo study on the rat heart functions: Hematopoietic and immune systems	96:172	Potential of chitosan nanoparticle/fluoride nanocomposite for reducing the toxicity of fluoride an in-vivo study on the rat heart functions: Hematopoietic and immune systems.					
35780919	7	12	with	chitosan	1381:1388	arg1	fluoride					1395:1402	fluoride	1395:1402	fluoride	1395:1402	The modified chitosan with fluoride decreases the toxicity of fluoride via improving the rat heart function due to the presence of Cs NPs helped to mitigate some of the negative effects of fluoride therapy.					
35780919	6	13	theme	multiple	1342:1349	arg1	test					1362:1365	Tukey's multiple comparison test	1334:1365	Tukey's multiple comparison test	1334:1365	To express all of the data, the mean and standard error of the mean are utilized by (ANOVA), followed by Tukey's multiple comparison test.					
35780919	3	14	theme	galvanized	847:856	arg1	cages					863:867	galvanized wire cages	847:867	galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod	847:922	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	1	15	theme	compatible	342:351	arg1	NaF					367:369	Cs@NaF	364:369	Cs@NaF	364:369	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	1	15	theme	compatible	342:351	arg1	composite					353:361	the biologically compatible composite	325:361	the biologically compatible composite (Cs@NaF)	325:370	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	6	16	theme	mean	1292:1295	arg1	error					1279:1283	the mean and standard error	1257:1283	the mean and standard error of the mean	1257:1295	To express all of the data, the mean and standard error of the mean are utilized by (ANOVA), followed by Tukey's multiple comparison test.					
35780919	3	17	theme	room	872:875	arg1	22-25 °C					890:897	22-25 °C	890:897	22-25 °C	890:897	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	3	17	theme	room	872:875	arg1	temperature					877:887	room temperature	872:887	room temperature (22-25 °C) with a 12-h photoperiod	872:922	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	7	18	theme	Cs	1499:1500	arg1	NPs					1502:1504	Cs NPs	1499:1504	Cs NPs helped to mitigate some of the negative effects of fluoride therapy	1499:1572	The modified chitosan with fluoride decreases the toxicity of fluoride via improving the rat heart function due to the presence of Cs NPs helped to mitigate some of the negative effects of fluoride therapy.					
35780919	3	19	theme	wire	858:861	arg1	cages					863:867	galvanized wire cages	847:867	galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod	847:922	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	4	20	theme	rat	1023:1025	arg1	heart					1027:1031	the rat heart	1019:1031	the rat heart	1019:1031	The blood samples were obtained from the vena cava of the rat heart via estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase.					
35780919	3	21	theme	body	664:667	arg1	weight					669:674	average body weight	656:674	average body weight of 150 ± 10 g	656:688	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	4	22	theme	troponin	1055:1062	arg1	T					1064:1064	troponin T	1055:1064	troponin T	1055:1064	The blood samples were obtained from the vena cava of the rat heart via estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase.					
35780919	2	23	theme	different	421:429	arg1	diffraction					531:541	x-ray diffraction	525:541	x-ray diffraction (XRD)	525:547	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	2	23	theme	different	421:429	arg1	microscopy					483:492	field emission scanning electron microscopy	450:492	field emission scanning electron microscopy (FEG-SEM)	450:502	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	2	23	theme	different	421:429	arg1	potential					510:518	zeta potential	505:518	zeta potential	505:518	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	2	23	theme	different	421:429	arg1	techniques					431:440	different techniques	421:440	different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD)	421:547	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	2	24	theme	emission	456:463	arg1	FEG-SEM					495:501	FEG-SEM	495:501	FEG-SEM	495:501	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	2	24	theme	emission	456:463	arg1	microscopy					483:492	field emission scanning electron microscopy	450:492	field emission scanning electron microscopy (FEG-SEM)	450:502	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	3	25	theme	150 ± 10 g	679:688	arg1	weight					669:674	average body weight	656:674	average body weight of 150 ± 10 g	656:688	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	1	26	theme	present	179:185	arg1	work-study					187:196	The present work-study	175:196	The present work-study	175:196	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	6	27	used	utilized	1301:1308	arg2	error					1279:1283	the mean and standard error	1257:1283	the mean and standard error of the mean	1257:1295	To express all of the data, the mean and standard error of the mean are utilized by (ANOVA), followed by Tukey's multiple comparison test.					
35780919	3	28	theme	adult	629:633	arg1	rats					650:653	twenty-eight male adult Sprague Dawley rats	611:653	twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g)	611:689	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	3	29	theme	Agriculture	725:735	arg1	Faculty					714:720	the Faculty	710:720	the Faculty of Agriculture, Alexandria University	710:758	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	0	30	theme	nanoparticle/fluoride	22:42	arg1	Potential					0:8	Potential	0:8	Potential of chitosan nanoparticle/fluoride	0:42	Potential of chitosan nanoparticle/fluoride nanocomposite for reducing the toxicity of fluoride an in-vivo study on the rat heart functions: Hematopoietic and immune systems.					
35780919	3	31	dep	rats	650:653	arg1	weight					669:674	average body weight	656:674	average body weight of 150 ± 10 g	656:688	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	0	32	theme	heart	124:128	arg1	functions					130:138	the rat heart functions	116:138	the rat heart functions: Hematopoietic and immune systems	116:172	Potential of chitosan nanoparticle/fluoride nanocomposite for reducing the toxicity of fluoride an in-vivo study on the rat heart functions: Hematopoietic and immune systems.					
35780919	6	33	theme	comparison	1351:1360	arg1	test					1362:1365	Tukey's multiple comparison test	1334:1365	Tukey's multiple comparison test	1334:1365	To express all of the data, the mean and standard error of the mean are utilized by (ANOVA), followed by Tukey's multiple comparison test.					
35780919	0	34	theme	chitosan	13:20	arg1	nanoparticle/fluoride					22:42	chitosan nanoparticle/fluoride	13:42	chitosan nanoparticle/fluoride	13:42	Potential of chitosan nanoparticle/fluoride nanocomposite for reducing the toxicity of fluoride an in-vivo study on the rat heart functions: Hematopoietic and immune systems.					
35780919	1	35	theme	chitosan	277:284	arg1	nanoparticles					286:298	chitosan nanoparticles	277:298	the chitosan nanoparticles (Cs NPs) surface	273:315	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	1	35	theme	chitosan	277:284	arg1	NPs					304:306	Cs NPs	301:306	Cs NPs	301:306	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	0	36	theme	rat	120:122	arg1	functions					130:138	the rat heart functions	116:138	the rat heart functions: Hematopoietic and immune systems	116:172	Potential of chitosan nanoparticle/fluoride nanocomposite for reducing the toxicity of fluoride an in-vivo study on the rat heart functions: Hematopoietic and immune systems.					
35780919	1	37	theme	Cs	364:365	arg1	NaF					367:369	Cs@NaF	364:369	Cs@NaF	364:369	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	1	37	theme	Cs	364:365	arg1	composite					353:361	the biologically compatible composite	325:361	the biologically compatible composite (Cs@NaF)	325:370	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	4	38	theme	heart	1027:1031	arg1	cava					1011:1014	the vena cava	1002:1014	the vena cava of the rat heart	1002:1031	The blood samples were obtained from the vena cava of the rat heart via estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase.					
35780919	4	39	theme	Lactate	1067:1073	arg1	dehydrogenase					1075:1087	Lactate dehydrogenase	1067:1087	Lactate dehydrogenase	1067:1087	The blood samples were obtained from the vena cava of the rat heart via estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase.					
35780919	2	40	theme	obtained	377:384	arg1	nanocomposite					386:398	The obtained nanocomposite	373:398	The obtained nanocomposite	373:398	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	2	41	theme	field	450:454	arg1	FEG-SEM					495:501	FEG-SEM	495:501	FEG-SEM	495:501	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	2	41	theme	field	450:454	arg1	microscopy					483:492	field emission scanning electron microscopy	450:492	field emission scanning electron microscopy (FEG-SEM)	450:502	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	1	42	theme	nanoparticles	286:298	arg1	surface					309:315	the chitosan nanoparticles (Cs NPs) surface	273:315	the chitosan nanoparticles (Cs NPs) surface	273:315	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	7	43	theme	NPs	1502:1504	arg1	presence					1487:1494	the presence	1483:1494	the presence of Cs NPs helped to mitigate some of the negative effects of fluoride therapy	1483:1572	The modified chitosan with fluoride decreases the toxicity of fluoride via improving the rat heart function due to the presence of Cs NPs helped to mitigate some of the negative effects of fluoride therapy.					
35780919	1	44	theme	@	366:366	arg1	NaF					367:369	Cs@NaF	364:369	Cs@NaF	364:369	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	1	44	theme	@	366:366	arg1	composite					353:361	the biologically compatible composite	325:361	the biologically compatible composite (Cs@NaF)	325:370	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	4	45	theme	blood	969:973	arg1	samples					975:981	The blood samples	965:981	The blood samples	965:981	The blood samples were obtained from the vena cava of the rat heart via estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase.					
35780919	1	46	theme	fluoride	213:220	arg1	toxicity					227:234	fluoride ions toxicity	213:234	fluoride ions toxicity on the rat heart	213:251	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	7	47	theme	therapy	1566:1572	arg1	effects					1546:1552	the negative effects	1533:1552	the negative effects of fluoride therapy	1533:1572	The modified chitosan with fluoride decreases the toxicity of fluoride via improving the rat heart function due to the presence of Cs NPs helped to mitigate some of the negative effects of fluoride therapy.					
35780919	7	48	theme	rat	1457:1459	arg1	heart					1461:1465	the rat heart	1453:1465	the rat heart function	1453:1474	The modified chitosan with fluoride decreases the toxicity of fluoride via improving the rat heart function due to the presence of Cs NPs helped to mitigate some of the negative effects of fluoride therapy.					
35780919	2	49	theme	zeta	505:508	arg1	potential					510:518	zeta potential	505:518	zeta potential	505:518	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	3	50	theme	biochemical	554:564	arg1	parameters					566:575	The biochemical parameters	550:575	The biochemical parameters	550:575	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	1	51	theme	ions	222:225	arg1	toxicity					227:234	fluoride ions toxicity	213:234	fluoride ions toxicity on the rat heart	213:251	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	6	52	theme	standard	1270:1277	arg1	error					1279:1283	the mean and standard error	1257:1283	the mean and standard error of the mean	1257:1295	To express all of the data, the mean and standard error of the mean are utilized by (ANOVA), followed by Tukey's multiple comparison test.					
35780919	1	53	theme	Cs	301:302	arg1	nanoparticles					286:298	chitosan nanoparticles	277:298	the chitosan nanoparticles (Cs NPs) surface	273:315	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	1	53	theme	Cs	301:302	arg1	NPs					304:306	Cs NPs	301:306	Cs NPs	301:306	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	7	54	theme	fluoride	1430:1437	arg1	toxicity					1418:1425	the toxicity	1414:1425	the toxicity of fluoride	1414:1437	The modified chitosan with fluoride decreases the toxicity of fluoride via improving the rat heart function due to the presence of Cs NPs helped to mitigate some of the negative effects of fluoride therapy.					
35780919	3	55	with	temperature	877:887	arg1	photoperiod					912:922	a 12-h photoperiod	905:922	a 12-h photoperiod	905:922	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	6	56	dep	utilized	1301:1308	arg1	followed					1322:1329	followed	1322:1329	followed by Tukey's multiple comparison test	1322:1365	To express all of the data, the mean and standard error of the mean are utilized by (ANOVA), followed by Tukey's multiple comparison test.					
35780919	1	57	from	loading	257:263	arg1	surface					309:315	the chitosan nanoparticles (Cs NPs) surface	273:315	the chitosan nanoparticles (Cs NPs) surface	273:315	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	1	58	dep	loading	257:263	arg1	them					265:268	them	265:268	them	265:268	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	3	59	from	temperature	877:887	arg1	groups					837:842	four groups	832:842	four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod	832:922	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	3	59	from	temperature	877:887	arg1	cages					863:867	galvanized wire cages	847:867	galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod	847:922	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	4	60	theme	phosphokinase	1103:1115	arg1	estimation					1037:1046	estimation	1037:1046	estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase	1037:1115	The blood samples were obtained from the vena cava of the rat heart via estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase.					
35780919	4	61	theme	vena	1006:1009	arg1	cava					1011:1014	the vena cava	1002:1014	the vena cava of the rat heart	1002:1031	The blood samples were obtained from the vena cava of the rat heart via estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase.					
35780919	1	62	from	toxicity	227:234	arg1	heart					247:251	the rat heart	239:251	the rat heart	239:251	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	5	63	theme	rat	1218:1220	arg1	heart					1222:1226	the rat heart	1214:1226	the rat heart	1214:1226	Also, immunoglobulins (IgA, IgM, and IgG) and hematological measurements have been performed on the rat heart.					
35780919	3	64	from	groups	837:842	arg1	22-25 °C					890:897	22-25 °C	890:897	22-25 °C	890:897	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	3	64	from	groups	837:842	arg1	cages					863:867	galvanized wire cages	847:867	galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod	847:922	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	3	64	from	groups	837:842	arg1	temperature					877:887	room temperature	872:887	room temperature (22-25 °C) with a 12-h photoperiod	872:922	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	4	65	theme	creatine	1094:1101	arg1	phosphokinase					1103:1115	creatine phosphokinase	1094:1115	creatine phosphokinase	1094:1115	The blood samples were obtained from the vena cava of the rat heart via estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase.					
35780919	5	66	theme	hematological	1164:1176	arg1	measurements					1178:1189	hematological measurements	1164:1189	hematological measurements	1164:1189	Also, immunoglobulins (IgA, IgM, and IgG) and hematological measurements have been performed on the rat heart.					
35780919	3	67	dep	rats	591:594	arg1	perform					596:602	perform	596:602	perform	596:602	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	0	68	theme	immune	159:164	arg1	systems					166:172	immune systems	159:172	immune systems	159:172	Potential of chitosan nanoparticle/fluoride nanocomposite for reducing the toxicity of fluoride an in-vivo study on the rat heart functions: Hematopoietic and immune systems.					
35780919	0	69	from	study	107:111	arg1	functions					130:138	the rat heart functions	116:138	the rat heart functions: Hematopoietic and immune systems	116:172	Potential of chitosan nanoparticle/fluoride nanocomposite for reducing the toxicity of fluoride an in-vivo study on the rat heart functions: Hematopoietic and immune systems.					
35780919	3	70	theme	albino	584:589	arg1	rats					591:594	the albino rats	580:594	the albino rats perform	580:602	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	3	71	from	parameters	566:575	arg1	rats					591:594	the albino rats	580:594	the albino rats perform	580:602	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	5	72	dep	immunoglobulins	1124:1138	arg1	IgG					1155:1157	IgG	1155:1157	IgG	1155:1157	Also, immunoglobulins (IgA, IgM, and IgG) and hematological measurements have been performed on the rat heart.					
35780919	5	72	dep	immunoglobulins	1124:1138	arg1	IgA					1141:1143	IgA	1141:1143	IgA	1141:1143	Also, immunoglobulins (IgA, IgM, and IgG) and hematological measurements have been performed on the rat heart.					
35780919	5	72	dep	immunoglobulins	1124:1138	arg1	immunoglobulins					1124:1138	immunoglobulins	1124:1138	immunoglobulins (IgA, IgM, and IgG)	1124:1158	Also, immunoglobulins (IgA, IgM, and IgG) and hematological measurements have been performed on the rat heart.					
35780919	5	72	dep	immunoglobulins	1124:1138	arg1	IgM					1146:1148	IgM	1146:1148	IgM	1146:1148	Also, immunoglobulins (IgA, IgM, and IgG) and hematological measurements have been performed on the rat heart.					
35780919	6	73	theme	mean	1261:1264	arg1	error					1279:1283	the mean and standard error	1257:1283	the mean and standard error of the mean	1257:1295	To express all of the data, the mean and standard error of the mean are utilized by (ANOVA), followed by Tukey's multiple comparison test.					
35780919	3	74	theme	male	624:627	arg1	rats					650:653	twenty-eight male adult Sprague Dawley rats	611:653	twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g)	611:689	The biochemical parameters in the albino rats perform, where twenty-eight male adult Sprague Dawley rats (average body weight of 150 ± 10 g) were obtained from the Faculty of Agriculture, Alexandria University, then acclimatized for two weeks before the experiment and divided into four groups in galvanized wire cages at room temperature (22-25 °C) with a 12-h photoperiod and fed a well-balanced commercial diet.					
35780919	4	75	theme	T	1064:1064	arg1	estimation					1037:1046	estimation	1037:1046	estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase	1037:1115	The blood samples were obtained from the vena cava of the rat heart via estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase.					
35780919	7	76	theme	fluoride	1557:1564	arg1	therapy					1566:1572	fluoride therapy	1557:1572	fluoride therapy	1557:1572	The modified chitosan with fluoride decreases the toxicity of fluoride via improving the rat heart function due to the presence of Cs NPs helped to mitigate some of the negative effects of fluoride therapy.					
35780919	2	77	theme	electron	474:481	arg1	FEG-SEM					495:501	FEG-SEM	495:501	FEG-SEM	495:501	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	2	77	theme	electron	474:481	arg1	microscopy					483:492	field emission scanning electron microscopy	450:492	field emission scanning electron microscopy (FEG-SEM)	450:502	The obtained nanocomposite was characterized by different techniques such as field emission scanning electron microscopy (FEG-SEM), zeta potential, and x-ray diffraction (XRD).					
35780919	1	78	dep	decreasing	202:211	arg1	the					198:200	the	198:200	the	198:200	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35780919	4	79	theme	dehydrogenase	1075:1087	arg1	estimation					1037:1046	estimation	1037:1046	estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase	1037:1115	The blood samples were obtained from the vena cava of the rat heart via estimation of the troponin T, Lactate dehydrogenase, and creatine phosphokinase.					
35780919	0	80	dep	functions	130:138	arg1	Hematopoietic					141:153	Hematopoietic	141:153	Hematopoietic	141:153	Potential of chitosan nanoparticle/fluoride nanocomposite for reducing the toxicity of fluoride an in-vivo study on the rat heart functions: Hematopoietic and immune systems.					
35780919	0	80	dep	functions	130:138	arg1	systems					166:172	immune systems	159:172	immune systems	159:172	Potential of chitosan nanoparticle/fluoride nanocomposite for reducing the toxicity of fluoride an in-vivo study on the rat heart functions: Hematopoietic and immune systems.					
35780919	7	81	theme	negative	1537:1544	arg1	effects					1546:1552	the negative effects	1533:1552	the negative effects of fluoride therapy	1533:1572	The modified chitosan with fluoride decreases the toxicity of fluoride via improving the rat heart function due to the presence of Cs NPs helped to mitigate some of the negative effects of fluoride therapy.					
35780919	1	82	theme	rat	243:245	arg1	heart					247:251	the rat heart	239:251	the rat heart	239:251	The present work-study the decreasing fluoride ions toxicity on the rat heart via loading them on the chitosan nanoparticles (Cs NPs) surface to form the biologically compatible composite (Cs@NaF).					
35543538	8	0	theme	prepared	1081:1088	arg1	scaffolds					1099:1107	prepared chitosan scaffolds	1081:1107	prepared chitosan scaffolds	1081:1107	Improvements in the physical properties of prepared chitosan scaffolds were concluded through higher water absorption and retention values.					
35543538	10	1	from	degrees	1406:1412	arg1	surface					1586:1592	scaffold surface	1577:1592	scaffold surface	1577:1592	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	10	2	theme	enzymatic	1373:1381	arg1	degradation					1383:1393	enzymatic degradation	1373:1393	enzymatic degradation	1373:1393	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	2	3	theme	modified	334:341	arg1	method					352:357	modified Hummer's method	334:357	modified Hummer's method	334:357	GO was synthesized according to modified Hummer's method and covalently linked to polymeric chains of PEG to produce polyethylene glycolated GO (PGO).					
35543538	8	4	theme	scaffolds	1099:1107	arg1	properties					1067:1076	the physical properties	1054:1076	the physical properties of prepared chitosan scaffolds	1054:1107	Improvements in the physical properties of prepared chitosan scaffolds were concluded through higher water absorption and retention values.					
35543538	10	5	theme	Cs	1536:1537	arg1	scaffold					1539:1546	Cs scaffold	1536:1546	Cs scaffold	1536:1546	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	11	6	theme	highest	1626:1632	arg1	degree					1634:1639	the highest degree	1622:1639	the highest degree of cell viability and lower hemolysis than both Cs and Cs-GO scaffolds	1622:1710	Also, Cs-PGO sample showed the highest degree of cell viability and lower hemolysis than both Cs and Cs-GO scaffolds.					
35543538	7	7	dep	higher	969:974	arg1	density					989:995	density	989:995	density	989:995	Modified samples offered higher porosity and density, indicating adequate scaffold structure.					
35543538	7	7	dep	higher	969:974	arg1	porosity					976:983	porosity	976:983	porosity	976:983	Modified samples offered higher porosity and density, indicating adequate scaffold structure.					
35543538	8	8	theme	higher	1132:1137	arg1	absorption					1145:1154	higher water absorption	1132:1154	higher water absorption	1132:1154	Improvements in the physical properties of prepared chitosan scaffolds were concluded through higher water absorption and retention values.					
35543538	10	9	theme	biomineral	1553:1562	arg1	formation					1564:1572	biomineral formation	1553:1572	biomineral formation on scaffold surface	1553:1592	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	7	10	theme	adequate	1009:1016	arg1	structure					1027:1035	adequate scaffold structure	1009:1035	adequate scaffold structure	1009:1035	Modified samples offered higher porosity and density, indicating adequate scaffold structure.					
35543538	5	11	theme	porous	780:785	arg1	scaffolds					787:795	The highly porous scaffolds	769:795	The highly porous scaffolds	769:795	The highly porous scaffolds were developed by lyophilization of solutions.					
35543538	6	12	theme	chitosan	877:884	arg1	network					895:901	chitosan scaffold network	877:901	chitosan scaffold network	877:901	Incorporation of GO and PGO into chitosan scaffold network resulted in uniform and spherical pores.					
35543538	9	13	theme	Compressive	1178:1188	arg1	measurement					1199:1209	Compressive strength measurement	1178:1209	Compressive strength measurement	1178:1209	Compressive strength measurement showed 6.33 and 5.5 times improvement respectively for Cs-GO and Cs-PGO samples compared to Cs scaffold.					
35543538	8	14	theme	retention	1160:1168	arg1	values					1170:1175	retention values	1160:1175	retention values	1160:1175	Improvements in the physical properties of prepared chitosan scaffolds were concluded through higher water absorption and retention values.					
35543538	6	15	theme	PGO	868:870	arg1	Incorporation					844:856	Incorporation	844:856	Incorporation of GO and PGO into chitosan scaffold network	844:901	Incorporation of GO and PGO into chitosan scaffold network resulted in uniform and spherical pores.					
35543538	6	16	theme	GO	861:862	arg1	Incorporation					844:856	Incorporation	844:856	Incorporation of GO and PGO into chitosan scaffold network	844:901	Incorporation of GO and PGO into chitosan scaffold network resulted in uniform and spherical pores.					
35543538	4	17	theme	Nanocomposite	654:666	arg1	scaffolds					668:676	Nanocomposite scaffolds	654:676	Nanocomposite scaffolds	654:676	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	3	18	theme	chemical	545:552	arg1	bonding					554:560	the chemical bonding	541:560	the chemical bonding of PEG and GO nanosheets	541:585	Successful preparation of GO and PGO was confirmed by FT-IR and Raman techniques, where the chemical bonding of PEG and GO nanosheets were concluded based on PGOs' lower zeta potential compared to GO.					
35543538	12	19	from	prominent	1798:1806	arg1	scaffolds					1818:1826	Cs-PGO scaffolds	1811:1826	Cs-PGO scaffolds	1811:1826	Investigations showed that cell infiltration into scaffold porous structure was more prominent in Cs-PGO scaffolds than in Cs and Cs-GO scaffolds.					
35543538	12	19	from	prominent	1798:1806	arg1	scaffolds					1849:1857	Cs and Cs-GO scaffolds	1836:1857	Cs and Cs-GO scaffolds	1836:1857	Investigations showed that cell infiltration into scaffold porous structure was more prominent in Cs-PGO scaffolds than in Cs and Cs-GO scaffolds.					
35543538	11	20	theme	Cs-GO	1696:1700	arg1	scaffolds					1702:1710	both Cs and Cs-GO scaffolds	1684:1710	both Cs and Cs-GO scaffolds	1684:1710	Also, Cs-PGO sample showed the highest degree of cell viability and lower hemolysis than both Cs and Cs-GO scaffolds.					
35543538	11	21	theme	Cs	1689:1690	arg1	scaffolds					1702:1710	both Cs and Cs-GO scaffolds	1684:1710	both Cs and Cs-GO scaffolds	1684:1710	Also, Cs-PGO sample showed the highest degree of cell viability and lower hemolysis than both Cs and Cs-GO scaffolds.					
35543538	0	22	from	effect	15:20	arg1	properties					58:67	the properties	54:67	the properties of chitosan-graphene oxide nanocomposite scaffold	54:117	Evaluating the effect of graphene oxide PEGylation on the properties of chitosan-graphene oxide nanocomposite scaffold.					
35543538	10	23	theme	protein	1417:1423	arg1	adsorption					1425:1434	protein adsorption	1417:1434	protein adsorption	1417:1434	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	0	24	theme	oxide	90:94	arg1	scaffold					110:117	chitosan-graphene oxide nanocomposite scaffold	72:117	chitosan-graphene oxide nanocomposite scaffold	72:117	Evaluating the effect of graphene oxide PEGylation on the properties of chitosan-graphene oxide nanocomposite scaffold.					
35543538	10	25	theme	23	1445:1446	arg1	%					1447:1447	%	1447:1447	%	1447:1447	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	0	26	theme	scaffold	110:117	arg1	properties					58:67	the properties	54:67	the properties of chitosan-graphene oxide nanocomposite scaffold	54:117	Evaluating the effect of graphene oxide PEGylation on the properties of chitosan-graphene oxide nanocomposite scaffold.					
35543538	12	27	theme	Cs-GO	1843:1847	arg1	scaffolds					1849:1857	Cs and Cs-GO scaffolds	1836:1857	Cs and Cs-GO scaffolds	1836:1857	Investigations showed that cell infiltration into scaffold porous structure was more prominent in Cs-PGO scaffolds than in Cs and Cs-GO scaffolds.					
35543538	9	28	dep	Cs-GO	1266:1270	arg1	samples					1283:1289	samples	1283:1289	samples	1283:1289	Compressive strength measurement showed 6.33 and 5.5 times improvement respectively for Cs-GO and Cs-PGO samples compared to Cs scaffold.					
35543538	4	29	theme	equal	702:706	arg1	PGO					726:728	PGO	726:728	PGO	726:728	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	4	29	theme	equal	702:706	arg1	GO					719:720	GO	719:720	GO	719:720	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	4	29	theme	equal	702:706	arg1	amounts					708:714	equal amounts	702:714	equal amounts of GO and PGO	702:728	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	2	30	theme	PEG	404:406	arg1	chains					394:399	polymeric chains	384:399	polymeric chains of PEG	384:406	GO was synthesized according to modified Hummer's method and covalently linked to polymeric chains of PEG to produce polyethylene glycolated GO (PGO).					
35543538	0	31	theme	graphene	25:32	arg1	PEGylation					40:49	graphene oxide PEGylation	25:49	graphene oxide PEGylation	25:49	Evaluating the effect of graphene oxide PEGylation on the properties of chitosan-graphene oxide nanocomposite scaffold.					
35543538	1	32	theme	nanocomposite	278:290	arg1	scaffold					292:299	chitosan-based nanocomposite scaffold	263:299	chitosan-based nanocomposite scaffold	263:299	In this study, graphene oxide (GO) was functionalized with polyethylene glycol (PEG) to understand the effect of PEGlayted GO on properties of chitosan-based nanocomposite scaffold.					
35543538	12	33	theme	Cs	1836:1837	arg1	scaffolds					1849:1857	Cs and Cs-GO scaffolds	1836:1857	Cs and Cs-GO scaffolds	1836:1857	Investigations showed that cell infiltration into scaffold porous structure was more prominent in Cs-PGO scaffolds than in Cs and Cs-GO scaffolds.					
35543538	11	34	theme	cell	1644:1647	arg1	viability					1649:1657	cell viability	1644:1657	cell viability	1644:1657	Also, Cs-PGO sample showed the highest degree of cell viability and lower hemolysis than both Cs and Cs-GO scaffolds.					
35543538	4	35	theme	chitosan	744:751	arg1	solutions					758:766	2% (w/v) chitosan (Cs) solutions	735:766	2% (w/v) chitosan (Cs) solutions	735:766	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	0	36	theme	PEGylation	40:49	arg1	effect					15:20	the effect	11:20	the effect of graphene oxide PEGylation on the properties of chitosan-graphene oxide nanocomposite scaffold	11:117	Evaluating the effect of graphene oxide PEGylation on the properties of chitosan-graphene oxide nanocomposite scaffold.					
35543538	2	37	attach	linked	374:379	arg2	GO					302:303	GO	302:303	GO	302:303	GO was synthesized according to modified Hummer's method and covalently linked to polymeric chains of PEG to produce polyethylene glycolated GO (PGO).					
35543538	2	37	attach	linked	374:379	arg1	chains					394:399	polymeric chains	384:399	polymeric chains of PEG	384:406	GO was synthesized according to modified Hummer's method and covalently linked to polymeric chains of PEG to produce polyethylene glycolated GO (PGO).					
35543538	10	38	theme	adsorbed	1473:1480	arg1	protein					1482:1488	adsorbed protein	1473:1488	adsorbed protein	1473:1488	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	3	39	theme	zeta	623:626	arg1	potential					628:636	PGOs' lower zeta potential	611:636	PGOs' lower zeta potential compared to GO	611:651	Successful preparation of GO and PGO was confirmed by FT-IR and Raman techniques, where the chemical bonding of PEG and GO nanosheets were concluded based on PGOs' lower zeta potential compared to GO.					
35543538	10	40	dep	degradation	1383:1393	arg1	%					1439:1439	26%	1437:1439	26%	1437:1439	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	10	40	dep	degradation	1383:1393	arg1	improvement					1449:1459	23% improvement	1445:1459	23% improvement in value of adsorbed protein	1445:1488	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	12	41	theme	Cs-PGO	1811:1816	arg1	scaffolds					1818:1826	Cs-PGO scaffolds	1811:1826	Cs-PGO scaffolds	1811:1826	Investigations showed that cell infiltration into scaffold porous structure was more prominent in Cs-PGO scaffolds than in Cs and Cs-GO scaffolds.					
35543538	9	42	dep	showed	1211:1216	arg1	compared					1291:1298	compared	1291:1298	compared to Cs scaffold	1291:1313	Compressive strength measurement showed 6.33 and 5.5 times improvement respectively for Cs-GO and Cs-PGO samples compared to Cs scaffold.					
35543538	11	43	theme	hemolysis	1669:1677	arg1	degree					1634:1639	the highest degree	1622:1639	the highest degree of cell viability and lower hemolysis than both Cs and Cs-GO scaffolds	1622:1710	Also, Cs-PGO sample showed the highest degree of cell viability and lower hemolysis than both Cs and Cs-GO scaffolds.					
35543538	4	44	theme	%	736:736	arg1	Cs					754:755	Cs	754:755	Cs	754:755	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	4	44	theme	%	736:736	arg1	chitosan					744:751	2% (w/v) chitosan	735:751	2% (w/v) chitosan (Cs) solutions	735:766	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	3	45	theme	PGO	486:488	arg1	preparation					464:474	Successful preparation	453:474	Successful preparation of GO and PGO	453:488	Successful preparation of GO and PGO was confirmed by FT-IR and Raman techniques, where the chemical bonding of PEG and GO nanosheets were concluded based on PGOs' lower zeta potential compared to GO.					
35543538	8	46	theme	physical	1058:1065	arg1	properties					1067:1076	the physical properties	1054:1076	the physical properties of prepared chitosan scaffolds	1054:1107	Improvements in the physical properties of prepared chitosan scaffolds were concluded through higher water absorption and retention values.					
35543538	12	47	theme	scaffold	1763:1770	arg1	structure					1779:1787	scaffold porous structure	1763:1787	scaffold porous structure	1763:1787	Investigations showed that cell infiltration into scaffold porous structure was more prominent in Cs-PGO scaffolds than in Cs and Cs-GO scaffolds.					
35543538	10	48	from	degradation	1383:1393	arg1	surface					1586:1592	scaffold surface	1577:1592	scaffold surface	1577:1592	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	3	49	theme	GO	479:480	arg1	preparation					464:474	Successful preparation	453:474	Successful preparation of GO and PGO	453:488	Successful preparation of GO and PGO was confirmed by FT-IR and Raman techniques, where the chemical bonding of PEG and GO nanosheets were concluded based on PGOs' lower zeta potential compared to GO.					
35543538	10	50	from	formation	1564:1572	arg1	surface					1586:1592	scaffold surface	1577:1592	scaffold surface	1577:1592	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	3	51	theme	FT-IR	507:511	arg1	techniques					523:532	FT-IR and Raman techniques	507:532	techniques	523:532	Successful preparation of GO and PGO was confirmed by FT-IR and Raman techniques, where the chemical bonding of PEG and GO nanosheets were concluded based on PGOs' lower zeta potential compared to GO.					
35543538	1	52	theme	PEGlayted	233:241	arg1	effect					223:228	the effect	219:228	the effect of PEGlayted GO on properties of chitosan-based nanocomposite scaffold	219:299	In this study, graphene oxide (GO) was functionalized with polyethylene glycol (PEG) to understand the effect of PEGlayted GO on properties of chitosan-based nanocomposite scaffold.					
35543538	8	53	theme	chitosan	1090:1097	arg1	scaffolds					1099:1107	prepared chitosan scaffolds	1081:1107	prepared chitosan scaffolds	1081:1107	Improvements in the physical properties of prepared chitosan scaffolds were concluded through higher water absorption and retention values.					
35543538	7	54	theme	scaffold	1018:1025	arg1	structure					1027:1035	adequate scaffold structure	1009:1035	adequate scaffold structure	1009:1035	Modified samples offered higher porosity and density, indicating adequate scaffold structure.					
35543538	10	55	theme	minimum	1343:1349	arg1	susceptibility					1351:1364	minimum susceptibility	1343:1364	minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface	1343:1592	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	4	56	dep	%	736:736	arg1	w/v					739:741	w/v	739:741	w/v	739:741	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	10	57	theme	higher	1399:1404	arg1	degrees					1406:1412	higher degrees	1399:1412	higher degrees of protein adsorption	1399:1434	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	6	58	theme	scaffold	886:893	arg1	network					895:901	chitosan scaffold network	877:901	chitosan scaffold network	877:901	Incorporation of GO and PGO into chitosan scaffold network resulted in uniform and spherical pores.					
35543538	3	59	theme	Raman	517:521	arg1	techniques					523:532	FT-IR and Raman techniques	507:532	techniques	523:532	Successful preparation of GO and PGO was confirmed by FT-IR and Raman techniques, where the chemical bonding of PEG and GO nanosheets were concluded based on PGOs' lower zeta potential compared to GO.					
35543538	3	60	dep	PEG	565:567	arg1	nanosheets					576:585	nanosheets	576:585	nanosheets	576:585	Successful preparation of GO and PGO was confirmed by FT-IR and Raman techniques, where the chemical bonding of PEG and GO nanosheets were concluded based on PGOs' lower zeta potential compared to GO.					
35543538	8	61	theme	water	1139:1143	arg1	absorption					1145:1154	higher water absorption	1132:1154	higher water absorption	1132:1154	Improvements in the physical properties of prepared chitosan scaffolds were concluded through higher water absorption and retention values.					
35543538	1	62	from	effect	223:228	arg1	properties					249:258	properties	249:258	properties of chitosan-based nanocomposite scaffold	249:299	In this study, graphene oxide (GO) was functionalized with polyethylene glycol (PEG) to understand the effect of PEGlayted GO on properties of chitosan-based nanocomposite scaffold.					
35543538	9	63	theme	strength	1190:1197	arg1	measurement					1199:1209	Compressive strength measurement	1178:1209	Compressive strength measurement	1178:1209	Compressive strength measurement showed 6.33 and 5.5 times improvement respectively for Cs-GO and Cs-PGO samples compared to Cs scaffold.					
35543538	10	64	from	%	1439:1439	arg1	value					1464:1468	value	1464:1468	value of adsorbed protein	1464:1488	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	10	65	theme	scaffold	1577:1584	arg1	surface					1586:1592	scaffold surface	1577:1592	scaffold surface	1577:1592	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	1	66	dep	PEGlayted	233:241	arg1	GO					243:244	GO	243:244	GO	243:244	In this study, graphene oxide (GO) was functionalized with polyethylene glycol (PEG) to understand the effect of PEGlayted GO on properties of chitosan-based nanocomposite scaffold.					
35543538	7	67	theme	Modified	944:951	arg1	samples					953:959	Modified samples	944:959	Modified samples	944:959	Modified samples offered higher porosity and density, indicating adequate scaffold structure.					
35543538	3	68	theme	GO	573:574	arg1	bonding					554:560	the chemical bonding	541:560	the chemical bonding of PEG and GO nanosheets	541:585	Successful preparation of GO and PGO was confirmed by FT-IR and Raman techniques, where the chemical bonding of PEG and GO nanosheets were concluded based on PGOs' lower zeta potential compared to GO.					
35543538	5	69	theme	solutions	833:841	arg1	lyophilization					815:828	lyophilization	815:828	lyophilization of solutions	815:841	The highly porous scaffolds were developed by lyophilization of solutions.					
35543538	10	70	from	improvement	1449:1459	arg1	value					1464:1468	value	1464:1468	value of adsorbed protein	1464:1488	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	3	71	theme	PEG	565:567	arg1	bonding					554:560	the chemical bonding	541:560	the chemical bonding of PEG and GO nanosheets	541:585	Successful preparation of GO and PGO was confirmed by FT-IR and Raman techniques, where the chemical bonding of PEG and GO nanosheets were concluded based on PGOs' lower zeta potential compared to GO.					
35543538	1	72	theme	polyethylene	179:190	arg1	glycol					192:197	polyethylene glycol	179:197	polyethylene glycol (PEG)	179:203	In this study, graphene oxide (GO) was functionalized with polyethylene glycol (PEG) to understand the effect of PEGlayted GO on properties of chitosan-based nanocomposite scaffold.					
35543538	1	72	theme	polyethylene	179:190	arg1	PEG					200:202	PEG	200:202	PEG	200:202	In this study, graphene oxide (GO) was functionalized with polyethylene glycol (PEG) to understand the effect of PEGlayted GO on properties of chitosan-based nanocomposite scaffold.					
35543538	1	73	theme	graphene	135:142	arg1	oxide					144:148	graphene oxide	135:148	graphene oxide (GO)	135:153	In this study, graphene oxide (GO) was functionalized with polyethylene glycol (PEG) to understand the effect of PEGlayted GO on properties of chitosan-based nanocomposite scaffold.					
35543538	6	74	theme	spherical	927:935	arg1	pores					937:941	uniform and spherical pores	915:941	uniform and spherical pores	915:941	Incorporation of GO and PGO into chitosan scaffold network resulted in uniform and spherical pores.					
35543538	12	75	from	scaffolds	1849:1857	arg1	prominent					1798:1806	prominent	1798:1806	prominent	1798:1806	Investigations showed that cell infiltration into scaffold porous structure was more prominent in Cs-PGO scaffolds than in Cs and Cs-GO scaffolds.					
35543538	0	76	theme	chitosan-graphene	72:88	arg1	scaffold					110:117	chitosan-graphene oxide nanocomposite scaffold	72:117	chitosan-graphene oxide nanocomposite scaffold	72:117	Evaluating the effect of graphene oxide PEGylation on the properties of chitosan-graphene oxide nanocomposite scaffold.					
35543538	12	77	from	scaffolds	1818:1826	arg1	prominent					1798:1806	prominent	1798:1806	prominent	1798:1806	Investigations showed that cell infiltration into scaffold porous structure was more prominent in Cs-PGO scaffolds than in Cs and Cs-GO scaffolds.					
35543538	6	78	theme	uniform	915:921	arg1	pores					937:941	uniform and spherical pores	915:941	uniform and spherical pores	915:941	Incorporation of GO and PGO into chitosan scaffold network resulted in uniform and spherical pores.					
35543538	10	79	theme	adsorption	1425:1434	arg1	formation					1564:1572	biomineral formation	1553:1572	biomineral formation on scaffold surface	1553:1592	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	10	79	theme	adsorption	1425:1434	arg1	degradation					1383:1393	enzymatic degradation	1373:1393	enzymatic degradation	1373:1393	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	10	79	theme	adsorption	1425:1434	arg1	degrees					1406:1412	higher degrees	1399:1412	higher degrees of protein adsorption	1399:1434	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	0	80	theme	nanocomposite	96:108	arg1	scaffold					110:117	chitosan-graphene oxide nanocomposite scaffold	72:117	chitosan-graphene oxide nanocomposite scaffold	72:117	Evaluating the effect of graphene oxide PEGylation on the properties of chitosan-graphene oxide nanocomposite scaffold.					
35543538	8	81	from	Improvements	1038:1049	arg1	properties					1067:1076	the physical properties	1054:1076	the physical properties of prepared chitosan scaffolds	1054:1107	Improvements in the physical properties of prepared chitosan scaffolds were concluded through higher water absorption and retention values.					
35543538	4	82	theme	GO	719:720	arg1	PGO					726:728	PGO	726:728	PGO	726:728	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	4	82	theme	GO	719:720	arg1	GO					719:720	GO	719:720	GO	719:720	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	4	82	theme	GO	719:720	arg1	amounts					708:714	equal amounts	702:714	equal amounts of GO and PGO	702:728	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	9	83	theme	Cs	1303:1304	arg1	scaffold					1306:1313	Cs scaffold	1303:1313	Cs scaffold	1303:1313	Compressive strength measurement showed 6.33 and 5.5 times improvement respectively for Cs-GO and Cs-PGO samples compared to Cs scaffold.					
35543538	11	84	theme	viability	1649:1657	arg1	degree					1634:1639	the highest degree	1622:1639	the highest degree of cell viability and lower hemolysis than both Cs and Cs-GO scaffolds	1622:1710	Also, Cs-PGO sample showed the highest degree of cell viability and lower hemolysis than both Cs and Cs-GO scaffolds.					
35543538	1	85	dep	oxide	144:148	arg1	GO					151:152	GO	151:152	GO	151:152	In this study, graphene oxide (GO) was functionalized with polyethylene glycol (PEG) to understand the effect of PEGlayted GO on properties of chitosan-based nanocomposite scaffold.					
35543538	0	86	theme	oxide	34:38	arg1	PEGylation					40:49	graphene oxide PEGylation	25:49	graphene oxide PEGylation	25:49	Evaluating the effect of graphene oxide PEGylation on the properties of chitosan-graphene oxide nanocomposite scaffold.					
35543538	1	87	theme	chitosan-based	263:276	arg1	scaffold					292:299	chitosan-based nanocomposite scaffold	263:299	chitosan-based nanocomposite scaffold	263:299	In this study, graphene oxide (GO) was functionalized with polyethylene glycol (PEG) to understand the effect of PEGlayted GO on properties of chitosan-based nanocomposite scaffold.					
35543538	10	88	theme	%	1447:1447	arg1	improvement					1449:1459	23% improvement	1445:1459	23% improvement in value of adsorbed protein	1445:1488	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	2	89	theme	polymeric	384:392	arg1	chains					394:399	polymeric chains	384:399	polymeric chains of PEG	384:406	GO was synthesized according to modified Hummer's method and covalently linked to polymeric chains of PEG to produce polyethylene glycolated GO (PGO).					
35543538	1	90	theme	scaffold	292:299	arg1	properties					249:258	properties	249:258	properties of chitosan-based nanocomposite scaffold	249:299	In this study, graphene oxide (GO) was functionalized with polyethylene glycol (PEG) to understand the effect of PEGlayted GO on properties of chitosan-based nanocomposite scaffold.					
35543538	12	91	theme	porous	1772:1777	arg1	structure					1779:1787	scaffold porous structure	1763:1787	scaffold porous structure	1763:1787	Investigations showed that cell infiltration into scaffold porous structure was more prominent in Cs-PGO scaffolds than in Cs and Cs-GO scaffolds.					
35543538	10	92	theme	protein	1482:1488	arg1	value					1464:1468	value	1464:1468	value of adsorbed protein	1464:1488	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35543538	3	93	theme	lower	617:621	arg1	potential					628:636	PGOs' lower zeta potential	611:636	PGOs' lower zeta potential compared to GO	611:651	Successful preparation of GO and PGO was confirmed by FT-IR and Raman techniques, where the chemical bonding of PEG and GO nanosheets were concluded based on PGOs' lower zeta potential compared to GO.					
35543538	11	94	theme	lower	1663:1667	arg1	hemolysis					1669:1677	lower hemolysis	1663:1677	lower hemolysis	1663:1677	Also, Cs-PGO sample showed the highest degree of cell viability and lower hemolysis than both Cs and Cs-GO scaffolds.					
35543538	4	95	theme	PGO	726:728	arg1	PGO					726:728	PGO	726:728	PGO	726:728	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	4	95	theme	PGO	726:728	arg1	GO					719:720	GO	719:720	GO	719:720	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	4	95	theme	PGO	726:728	arg1	amounts					708:714	equal amounts	702:714	equal amounts of GO and PGO	702:728	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	3	96	theme	Successful	453:462	arg1	preparation					464:474	Successful preparation	453:474	Successful preparation of GO and PGO	453:488	Successful preparation of GO and PGO was confirmed by FT-IR and Raman techniques, where the chemical bonding of PEG and GO nanosheets were concluded based on PGOs' lower zeta potential compared to GO.					
35543538	12	97	theme	cell	1740:1743	arg1	infiltration					1745:1756	cell infiltration	1740:1756	cell infiltration into scaffold porous structure	1740:1787	Investigations showed that cell infiltration into scaffold porous structure was more prominent in Cs-PGO scaffolds than in Cs and Cs-GO scaffolds.					
35543538	11	98	theme	Cs-PGO	1601:1606	arg1	sample					1608:1613	Cs-PGO sample	1601:1613	Cs-PGO sample	1601:1613	Also, Cs-PGO sample showed the highest degree of cell viability and lower hemolysis than both Cs and Cs-GO scaffolds.					
35543538	4	99	theme	2	735:735	arg1	%					736:736	%	736:736	%	736:736	Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions.					
35543538	10	100	theme	Cs-GO	1320:1324	arg1	scaffolds					1326:1334	The Cs-GO scaffolds	1316:1334	The Cs-GO scaffolds	1316:1334	The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface.					
35379265	12	0	theme	PSCI	1790:1793	arg1	mice					1795:1798	PSCI mice	1790:1798	PSCI mice	1790:1798	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	13	1	theme	LPS	1860:1862	arg1	injection					1847:1855	Intraperitoneal injection	1831:1855	Intraperitoneal injection of LPS after stroke	1831:1875	Intraperitoneal injection of LPS after stroke caused similar pathology to those seen in PSCI mice.					
35379265	12	2	theme	Aβ	1757:1758	arg1	deposition					1760:1769	Aβ deposition	1757:1769	Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice	1757:1828	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	12	3	from	activation	1684:1693	arg1	thalamus					1778:1785	the thalamus	1774:1785	the thalamus of PSCI mice in comparison with nPSCI mice	1774:1828	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	12	3	from	activation	1684:1693	arg1	region					1726:1731	the CA1 region	1718:1731	the CA1 region of the hippocampus	1718:1750	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	11	4	theme	lower	1439:1443	arg1	level					1445:1449	a lower level	1437:1449	a lower level of fecal butyrate	1437:1467	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	12	5	theme	microglial	1673:1682	arg1	activation					1684:1693	microglial activation	1673:1693	microglial activation	1673:1693	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	7	6	dep	gut	827:829	arg1	microbiota					831:840	microbiota	831:840	microbiota	831:840	Fecal microbiota transplantation from patients to stroke mice was performed to examine the causal relationship between the gut microbiota and PSCI.					
35379265	6	7	theme	factor	627:632	arg1	levels					634:639	The peripheral inflammatory factor levels	599:639	The peripheral inflammatory factor levels	599:639	The peripheral inflammatory factor levels and gut microbiota compositions of the patients were analyzed.					
35379265	11	8	theme	nPSCI	1581:1585	arg1	patients					1571:1578	nPSCI patients	1565:1578	nPSCI patients (nPSCI mice)	1565:1591	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	8	theme	nPSCI	1581:1585	arg1	mice					1587:1590	nPSCI mice	1581:1590	nPSCI mice	1581:1590	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	3	9	theme	microbiota	289:298	arg1	dysbiosis					300:308	gut microbiota dysbiosis	285:308	gut microbiota dysbiosis which affects brain injury	285:335	We previously found that stroke induces gut microbiota dysbiosis which affects brain injury.					
35379265	6	10	theme	peripheral	603:612	arg1	levels					634:639	The peripheral inflammatory factor levels	599:639	The peripheral inflammatory factor levels	599:639	The peripheral inflammatory factor levels and gut microbiota compositions of the patients were analyzed.					
35379265	5	11	theme	cognitive	490:498	arg1	function					500:507	their cognitive function	484:507	their cognitive function	484:507	METHODS 83 stroke patients were recruited and their cognitive function were measured by Montreal Cognitive Assessment (MoCA) scores 3 months after stroke onset.					
35379265	12	12	theme	CA1	1722:1724	arg1	region					1726:1731	the CA1 region	1718:1731	the CA1 region of the hippocampus	1718:1750	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	10	13	theme	PSCI	1043:1046	arg1	patients					1048:1055	PSCI patients	1043:1055	PSCI patients	1043:1055	Compared with non-PSCI patients, PSCI patients showed significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers.					
35379265	3	14	theme	brain	324:328	arg1	injury					330:335	brain injury	324:335	brain injury	324:335	We previously found that stroke induces gut microbiota dysbiosis which affects brain injury.					
35379265	7	15	theme	Fecal	704:708	arg1	transplantation					721:735	Fecal microbiota transplantation	704:735	Fecal microbiota transplantation from patients to stroke mice	704:764	Fecal microbiota transplantation from patients to stroke mice was performed to examine the causal relationship between the gut microbiota and PSCI.					
35379265	11	16	theme	cognitive	1504:1512	arg1	impairment					1514:1523	cognitive impairment	1504:1523	cognitive impairment	1504:1523	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	8	17	theme	cognitive	856:864	arg1	function					866:873	The cognitive function	852:873	The cognitive function of mice	852:881	The cognitive function of mice was evaluated by Morris water maze test.					
35379265	4	18	theme	PSCI	432:435	arg1	pathogenesis					416:427	the pathogenesis	412:427	the pathogenesis of PSCI	412:435	Hereby, we aimed to investigate whether the gut microbiota contributes to the pathogenesis of PSCI.					
35379265	11	19	from	patients	1242:1249	arg1	microbiota					1221:1230	microbiota	1221:1230	microbiota from PSCI patients (PSCI mice)	1221:1261	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	13	20	theme	PSCI	1919:1922	arg1	mice					1924:1927	PSCI mice	1919:1927	PSCI mice	1919:1927	Intraperitoneal injection of LPS after stroke caused similar pathology to those seen in PSCI mice.					
35379265	10	21	theme	higher	1078:1083	arg1	levels					1085:1090	significantly higher levels	1064:1090	significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers	1064:1178	Compared with non-PSCI patients, PSCI patients showed significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers.					
35379265	5	22	theme	Cognitive	535:543	arg1	scores					563:568	Montreal Cognitive Assessment (MoCA) scores	526:568	Montreal Cognitive Assessment (MoCA) scores 3 months after stroke onset	526:596	METHODS 83 stroke patients were recruited and their cognitive function were measured by Montreal Cognitive Assessment (MoCA) scores 3 months after stroke onset.					
35379265	6	23	theme	patients	680:687	arg1	compositions					660:671	gut microbiota compositions	645:671	gut microbiota compositions	645:671	The peripheral inflammatory factor levels and gut microbiota compositions of the patients were analyzed.					
35379265	6	23	theme	patients	680:687	arg1	levels					634:639	The peripheral inflammatory factor levels	599:639	The peripheral inflammatory factor levels	599:639	The peripheral inflammatory factor levels and gut microbiota compositions of the patients were analyzed.					
35379265	10	24	theme	lipopolysaccharide	1119:1136	arg1	levels					1085:1090	significantly higher levels	1064:1090	significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers	1064:1178	Compared with non-PSCI patients, PSCI patients showed significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers.					
35379265	12	25	theme	neuronal	1696:1703	arg1	apoptosis					1705:1713	neuronal apoptosis	1696:1713	neuronal apoptosis in the CA1 region of the hippocampus	1696:1750	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	11	26	theme	Toll-like	1322:1330	arg1	TLR4					1344:1347	TLR4	1344:1347	TLR4	1344:1347	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	26	theme	Toll-like	1322:1330	arg1	receptor-4					1332:1341	intestinal Toll-like receptor-4	1311:1341	intestinal Toll-like receptor-4 (TLR4) expression	1311:1359	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	6	27	theme	microbiota	649:658	arg1	compositions					660:671	gut microbiota compositions	645:671	gut microbiota compositions	645:671	The peripheral inflammatory factor levels and gut microbiota compositions of the patients were analyzed.					
35379265	10	28	theme	Enterobacteriaceae	1099:1116	arg1	levels					1085:1090	significantly higher levels	1064:1090	significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers	1064:1178	Compared with non-PSCI patients, PSCI patients showed significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers.					
35379265	1	29	from	prevalent	169:177	arg1	patients					189:196	stroke patients	182:196	stroke patients	182:196	BACKGROUND Poststroke cognitive impairment (PSCI) is prevalent in stroke patients.					
35379265	14	30	theme	detrimental	2006:2016	arg1	changes					2018:2024	these detrimental changes	2000:2024	these detrimental changes	2000:2024	Supplementation with sodium butyrate (NaB) via drinking water rescued these detrimental changes in PSCI mice.					
35379265	8	31	theme	water	907:911	arg1	test					918:921	Morris water maze test	900:921	Morris water maze test	900:921	The cognitive function of mice was evaluated by Morris water maze test.					
35379265	7	32	from	patients	742:749	arg1	transplantation					721:735	Fecal microbiota transplantation	704:735	Fecal microbiota transplantation from patients to stroke mice	704:764	Fecal microbiota transplantation from patients to stroke mice was performed to examine the causal relationship between the gut microbiota and PSCI.					
35379265	11	33	theme	butyrate	1460:1467	arg1	level					1445:1449	a lower level	1437:1449	a lower level of fecal butyrate	1437:1467	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	33	theme	butyrate	1460:1467	arg1	cytokines					1422:1430	inflammatory cytokines	1409:1430	inflammatory cytokines	1409:1430	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	33	theme	butyrate	1460:1467	arg1	protein					1391:1397	LPS-binding protein	1379:1397	LPS-binding protein (LBP)	1379:1403	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	33	theme	butyrate	1460:1467	arg1	LBP					1400:1402	LBP	1400:1402	LBP	1400:1402	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	33	theme	butyrate	1460:1467	arg1	impairment					1514:1523	cognitive impairment	1504:1523	cognitive impairment	1504:1523	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	33	theme	butyrate	1460:1467	arg1	LPS					1374:1376	LPS	1374:1376	LPS	1374:1376	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	33	theme	butyrate	1460:1467	arg1	destruction					1488:1498	severer intestine destruction	1470:1498	severer intestine destruction	1470:1498	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	5	34	theme	stroke	585:590	arg1	onset					592:596	stroke onset	585:596	stroke onset	585:596	METHODS 83 stroke patients were recruited and their cognitive function were measured by Montreal Cognitive Assessment (MoCA) scores 3 months after stroke onset.					
35379265	0	35	theme	Gut	0:2	arg1	microbiota					4:13	Gut microbiota	0:13	Gut microbiota	0:13	Gut microbiota is causally associated with poststroke cognitive impairment through lipopolysaccharide and butyrate.					
35379265	11	36	theme	PSCI	1252:1255	arg1	patients					1242:1249	PSCI patients	1237:1249	PSCI patients (PSCI mice)	1237:1261	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	36	theme	PSCI	1252:1255	arg1	mice					1257:1260	PSCI mice	1252:1260	PSCI mice	1252:1260	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	10	37	theme	inflammation	1159:1170	arg1	markers					1172:1178	peripheral inflammation markers	1148:1178	peripheral inflammation markers	1148:1178	Compared with non-PSCI patients, PSCI patients showed significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers.					
35379265	1	38	theme	Poststroke	127:136	arg1	PSCI					160:163	PSCI	160:163	PSCI	160:163	BACKGROUND Poststroke cognitive impairment (PSCI) is prevalent in stroke patients.					
35379265	1	38	theme	Poststroke	127:136	arg1	impairment					148:157	BACKGROUND Poststroke cognitive impairment	116:157	BACKGROUND Poststroke cognitive impairment (PSCI)	116:164	BACKGROUND Poststroke cognitive impairment (PSCI) is prevalent in stroke patients.					
35379265	11	39	theme	severer	1470:1476	arg1	destruction					1488:1498	severer intestine destruction	1470:1498	severer intestine destruction	1470:1498	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	0	40	theme	poststroke	43:52	arg1	impairment					64:73	poststroke cognitive impairment	43:73	poststroke cognitive impairment	43:73	Gut microbiota is causally associated with poststroke cognitive impairment through lipopolysaccharide and butyrate.					
35379265	14	41	theme	sodium	1951:1956	arg1	butyrate					1958:1965	sodium butyrate	1951:1965	sodium butyrate (NaB)	1951:1971	Supplementation with sodium butyrate (NaB) via drinking water rescued these detrimental changes in PSCI mice.					
35379265	14	41	theme	sodium	1951:1956	arg1	NaB					1968:1970	NaB	1968:1970	NaB	1968:1970	Supplementation with sodium butyrate (NaB) via drinking water rescued these detrimental changes in PSCI mice.					
35379265	12	42	theme	nPSCI	1819:1823	arg1	mice					1825:1828	nPSCI mice	1819:1828	nPSCI mice	1819:1828	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	11	43	theme	higher	1275:1280	arg1	level					1282:1286	a higher level	1273:1286	a higher level of Enterobacteriaceae	1273:1308	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	43	theme	higher	1275:1280	arg1	expression					1350:1359	intestinal Toll-like receptor-4 (TLR4) expression	1311:1359	intestinal Toll-like receptor-4 (TLR4) expression	1311:1359	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	12	44	theme	hippocampus	1740:1750	arg1	region					1726:1731	the CA1 region	1718:1731	the CA1 region of the hippocampus	1718:1750	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	10	45	theme	non-PSCI	1024:1031	arg1	patients					1033:1040	non-PSCI patients	1024:1040	non-PSCI patients	1024:1040	Compared with non-PSCI patients, PSCI patients showed significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers.					
35379265	11	46	theme	inflammatory	1409:1420	arg1	cytokines					1422:1430	inflammatory cytokines	1409:1430	inflammatory cytokines	1409:1430	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	15	47	theme	cause-effect	2072:2083	arg1	relationship					2085:2096	a cause-effect relationship	2070:2096	a cause-effect relationship between gut microbiota and PSCI for the first time, which is likely mediated by inflammation-regulating metabolites including LPS and butyrate	2070:2239	CONCLUSIONS Our data indicate a cause-effect relationship between gut microbiota and PSCI for the first time, which is likely mediated by inflammation-regulating metabolites including LPS and butyrate.					
35379265	12	48	theme	mice	1795:1798	arg1	thalamus					1778:1785	the thalamus	1774:1785	the thalamus of PSCI mice in comparison with nPSCI mice	1774:1828	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	12	49	from	thalamus	1778:1785	arg1	comparison					1803:1812	comparison	1803:1812	comparison with nPSCI mice	1803:1828	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	14	50	theme	drinking	1977:1984	arg1	water					1986:1990	drinking water	1977:1990	drinking water	1977:1990	Supplementation with sodium butyrate (NaB) via drinking water rescued these detrimental changes in PSCI mice.					
35379265	1	51	from	patients	189:196	arg1	prevalent					169:177	prevalent	169:177	prevalent	169:177	BACKGROUND Poststroke cognitive impairment (PSCI) is prevalent in stroke patients.					
35379265	7	52	theme	stroke	754:759	arg1	mice					761:764	stroke mice	754:764	stroke mice	754:764	Fecal microbiota transplantation from patients to stroke mice was performed to examine the causal relationship between the gut microbiota and PSCI.					
35379265	11	53	theme	nPSCI	1565:1569	arg1	patients					1571:1578	nPSCI patients	1565:1578	nPSCI patients (nPSCI mice)	1565:1591	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	53	theme	nPSCI	1565:1569	arg1	mice					1587:1590	nPSCI mice	1581:1590	nPSCI mice	1581:1590	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	1	54	theme	stroke	182:187	arg1	patients					189:196	stroke patients	182:196	stroke patients	182:196	BACKGROUND Poststroke cognitive impairment (PSCI) is prevalent in stroke patients.					
35379265	9	55	dep	RESULTS	924:930	arg1	classified					963:972	classified	963:972	classified	963:972	RESULTS 34 and 49 stroke patients were classified as PSCI and non-PSCI, respectively.					
35379265	4	56	theme	gut	382:384	arg1	microbiota					386:395	the gut microbiota	378:395	the gut microbiota	378:395	Hereby, we aimed to investigate whether the gut microbiota contributes to the pathogenesis of PSCI.					
35379265	5	57	theme	Montreal	526:533	arg1	scores					563:568	Montreal Cognitive Assessment (MoCA) scores	526:568	Montreal Cognitive Assessment (MoCA) scores 3 months after stroke onset	526:596	METHODS 83 stroke patients were recruited and their cognitive function were measured by Montreal Cognitive Assessment (MoCA) scores 3 months after stroke onset.					
35379265	12	58	with	comparison	1803:1812	arg1	mice					1825:1828	nPSCI mice	1819:1828	nPSCI mice	1819:1828	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	6	59	theme	inflammatory	614:625	arg1	levels					634:639	The peripheral inflammatory factor levels	599:639	The peripheral inflammatory factor levels	599:639	The peripheral inflammatory factor levels and gut microbiota compositions of the patients were analyzed.					
35379265	15	60	theme	first	2138:2142	arg1	time					2144:2147	the first time	2134:2147	the first time	2134:2147	CONCLUSIONS Our data indicate a cause-effect relationship between gut microbiota and PSCI for the first time, which is likely mediated by inflammation-regulating metabolites including LPS and butyrate.					
35379265	12	61	theme	blood-brain	1636:1646	arg1	BBB					1657:1659	BBB	1657:1659	BBB	1657:1659	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	12	61	theme	blood-brain	1636:1646	arg1	barrier					1648:1654	blood-brain barrier	1636:1654	blood-brain barrier (BBB) integrity	1636:1670	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	1	62	theme	BACKGROUND	116:125	arg1	PSCI					160:163	PSCI	160:163	PSCI	160:163	BACKGROUND Poststroke cognitive impairment (PSCI) is prevalent in stroke patients.					
35379265	1	62	theme	BACKGROUND	116:125	arg1	impairment					148:157	BACKGROUND Poststroke cognitive impairment	116:157	BACKGROUND Poststroke cognitive impairment (PSCI)	116:164	BACKGROUND Poststroke cognitive impairment (PSCI) is prevalent in stroke patients.					
35379265	3	63	theme	gut	285:287	arg1	dysbiosis					300:308	gut microbiota dysbiosis	285:308	gut microbiota dysbiosis which affects brain injury	285:335	We previously found that stroke induces gut microbiota dysbiosis which affects brain injury.					
35379265	11	64	theme	LPS-binding	1379:1389	arg1	protein					1391:1397	LPS-binding protein	1379:1397	LPS-binding protein (LBP)	1379:1403	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	64	theme	LPS-binding	1379:1389	arg1	LBP					1400:1402	LBP	1400:1402	LBP	1400:1402	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	14	65	theme	PSCI	2029:2032	arg1	mice					2034:2037	PSCI mice	2029:2037	PSCI mice	2029:2037	Supplementation with sodium butyrate (NaB) via drinking water rescued these detrimental changes in PSCI mice.					
35379265	1	66	theme	cognitive	138:146	arg1	PSCI					160:163	PSCI	160:163	PSCI	160:163	BACKGROUND Poststroke cognitive impairment (PSCI) is prevalent in stroke patients.					
35379265	1	66	theme	cognitive	138:146	arg1	impairment					148:157	BACKGROUND Poststroke cognitive impairment	116:157	BACKGROUND Poststroke cognitive impairment (PSCI)	116:164	BACKGROUND Poststroke cognitive impairment (PSCI) is prevalent in stroke patients.					
35379265	2	67	theme	PSCI	215:218	arg1	etiology					203:210	The etiology	199:210	The etiology of PSCI	199:218	The etiology of PSCI remains largely unknown.					
35379265	7	68	theme	microbiota	710:719	arg1	transplantation					721:735	Fecal microbiota transplantation	704:735	Fecal microbiota transplantation from patients to stroke mice	704:764	Fecal microbiota transplantation from patients to stroke mice was performed to examine the causal relationship between the gut microbiota and PSCI.					
35379265	12	69	from	apoptosis	1705:1713	arg1	thalamus					1778:1785	the thalamus	1774:1785	the thalamus of PSCI mice in comparison with nPSCI mice	1774:1828	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	12	69	from	apoptosis	1705:1713	arg1	region					1726:1731	the CA1 region	1718:1731	the CA1 region of the hippocampus	1718:1750	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	5	70	theme	Assessment	545:554	arg1	scores					563:568	Montreal Cognitive Assessment (MoCA) scores	526:568	Montreal Cognitive Assessment (MoCA) scores 3 months after stroke onset	526:596	METHODS 83 stroke patients were recruited and their cognitive function were measured by Montreal Cognitive Assessment (MoCA) scores 3 months after stroke onset.					
35379265	15	71	theme	inflammation-regulating	2178:2200	arg1	LPS					2224:2226	LPS	2224:2226	LPS	2224:2226	CONCLUSIONS Our data indicate a cause-effect relationship between gut microbiota and PSCI for the first time, which is likely mediated by inflammation-regulating metabolites including LPS and butyrate.					
35379265	15	71	theme	inflammation-regulating	2178:2200	arg1	metabolites					2202:2212	inflammation-regulating metabolites	2178:2212	inflammation-regulating metabolites including LPS and butyrate	2178:2239	CONCLUSIONS Our data indicate a cause-effect relationship between gut microbiota and PSCI for the first time, which is likely mediated by inflammation-regulating metabolites including LPS and butyrate.					
35379265	15	71	theme	inflammation-regulating	2178:2200	arg1	butyrate					2232:2239	butyrate	2232:2239	butyrate	2232:2239	CONCLUSIONS Our data indicate a cause-effect relationship between gut microbiota and PSCI for the first time, which is likely mediated by inflammation-regulating metabolites including LPS and butyrate.					
35379265	5	72	theme	MoCA	557:560	arg1	scores					563:568	Montreal Cognitive Assessment (MoCA) scores	526:568	Montreal Cognitive Assessment (MoCA) scores 3 months after stroke onset	526:596	METHODS 83 stroke patients were recruited and their cognitive function were measured by Montreal Cognitive Assessment (MoCA) scores 3 months after stroke onset.					
35379265	11	73	from	patients	1571:1578	arg1	microbiota					1549:1558	microbiota	1549:1558	microbiota from nPSCI patients (nPSCI mice)	1549:1591	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	8	74	theme	mice	878:881	arg1	function					866:873	The cognitive function	852:873	The cognitive function of mice	852:881	The cognitive function of mice was evaluated by Morris water maze test.					
35379265	11	75	theme	receptor-4	1332:1341	arg1	level					1282:1286	a higher level	1273:1286	a higher level of Enterobacteriaceae	1273:1308	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	75	theme	receptor-4	1332:1341	arg1	expression					1350:1359	intestinal Toll-like receptor-4 (TLR4) expression	1311:1359	intestinal Toll-like receptor-4 (TLR4) expression	1311:1359	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	6	76	theme	gut	645:647	arg1	compositions					660:671	gut microbiota compositions	645:671	gut microbiota compositions	645:671	The peripheral inflammatory factor levels and gut microbiota compositions of the patients were analyzed.					
35379265	10	77	theme	gut	1095:1097	arg1	Enterobacteriaceae					1099:1116	gut Enterobacteriaceae	1095:1116	gut Enterobacteriaceae	1095:1116	Compared with non-PSCI patients, PSCI patients showed significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers.					
35379265	11	78	theme	intestinal	1311:1320	arg1	TLR4					1344:1347	TLR4	1344:1347	TLR4	1344:1347	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	78	theme	intestinal	1311:1320	arg1	receptor-4					1332:1341	intestinal Toll-like receptor-4	1311:1341	intestinal Toll-like receptor-4 (TLR4) expression	1311:1359	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	79	theme	fecal	1454:1458	arg1	butyrate					1460:1467	fecal butyrate	1454:1467	fecal butyrate	1454:1467	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	13	80	theme	similar	1884:1890	arg1	pathology					1892:1900	similar pathology	1884:1900	similar pathology	1884:1900	Intraperitoneal injection of LPS after stroke caused similar pathology to those seen in PSCI mice.					
35379265	8	81	theme	Morris	900:905	arg1	test					918:921	Morris water maze test	900:921	Morris water maze test	900:921	The cognitive function of mice was evaluated by Morris water maze test.					
35379265	5	82	dep	METHODS	438:444	arg1	patients					456:463	83 stroke patients	446:463	METHODS 83 stroke patients	438:463	METHODS 83 stroke patients were recruited and their cognitive function were measured by Montreal Cognitive Assessment (MoCA) scores 3 months after stroke onset.					
35379265	14	83	with	Supplementation	1930:1944	arg1	butyrate					1958:1965	sodium butyrate	1951:1965	sodium butyrate (NaB)	1951:1971	Supplementation with sodium butyrate (NaB) via drinking water rescued these detrimental changes in PSCI mice.					
35379265	14	83	with	Supplementation	1930:1944	arg1	NaB					1968:1970	NaB	1968:1970	NaB	1968:1970	Supplementation with sodium butyrate (NaB) via drinking water rescued these detrimental changes in PSCI mice.					
35379265	8	84	theme	maze	913:916	arg1	test					918:921	Morris water maze test	900:921	Morris water maze test	900:921	The cognitive function of mice was evaluated by Morris water maze test.					
35379265	12	85	from	deposition	1760:1769	arg1	thalamus					1778:1785	the thalamus	1774:1785	the thalamus of PSCI mice in comparison with nPSCI mice	1774:1828	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	12	85	from	deposition	1760:1769	arg1	region					1726:1731	the CA1 region	1718:1731	the CA1 region of the hippocampus	1718:1750	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	11	86	theme	intestine	1478:1486	arg1	destruction					1488:1498	severer intestine destruction	1470:1498	severer intestine destruction	1470:1498	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	10	87	theme	markers	1172:1178	arg1	levels					1085:1090	significantly higher levels	1064:1090	significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers	1064:1178	Compared with non-PSCI patients, PSCI patients showed significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers.					
35379265	11	88	theme	PSCI	1237:1240	arg1	patients					1242:1249	PSCI patients	1237:1249	PSCI patients (PSCI mice)	1237:1261	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	88	theme	PSCI	1237:1240	arg1	mice					1257:1260	PSCI mice	1252:1260	PSCI mice	1252:1260	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	12	89	theme	barrier	1648:1654	arg1	integrity					1662:1670	blood-brain barrier (BBB) integrity	1636:1670	blood-brain barrier (BBB) integrity	1636:1670	In addition, we observed exacerbations in blood-brain barrier (BBB) integrity, microglial activation, neuronal apoptosis in the CA1 region of the hippocampus, and Aβ deposition in the thalamus of PSCI mice in comparison with nPSCI mice.					
35379265	13	90	theme	Intraperitoneal	1831:1845	arg1	injection					1847:1855	Intraperitoneal injection	1831:1855	Intraperitoneal injection of LPS after stroke	1831:1875	Intraperitoneal injection of LPS after stroke caused similar pathology to those seen in PSCI mice.					
35379265	10	91	theme	peripheral	1148:1157	arg1	markers					1172:1178	peripheral inflammation markers	1148:1178	peripheral inflammation markers	1148:1178	Compared with non-PSCI patients, PSCI patients showed significantly higher levels of gut Enterobacteriaceae, lipopolysaccharide (LPS) and peripheral inflammation markers.					
35379265	0	92	theme	cognitive	54:62	arg1	impairment					64:73	poststroke cognitive impairment	43:73	poststroke cognitive impairment	43:73	Gut microbiota is causally associated with poststroke cognitive impairment through lipopolysaccharide and butyrate.					
35379265	15	93	theme	gut	2106:2108	arg1	microbiota					2110:2119	gut microbiota	2106:2119	gut microbiota	2106:2119	CONCLUSIONS Our data indicate a cause-effect relationship between gut microbiota and PSCI for the first time, which is likely mediated by inflammation-regulating metabolites including LPS and butyrate.					
35379265	15	94	dep	CONCLUSIONS	2040:2050	arg1	indicate					2061:2068	indicate	2061:2068	indicate a cause-effect relationship between gut microbiota and PSCI for the first time, which is likely mediated by inflammation-regulating metabolites including LPS and butyrate	2061:2239	CONCLUSIONS Our data indicate a cause-effect relationship between gut microbiota and PSCI for the first time, which is likely mediated by inflammation-regulating metabolites including LPS and butyrate.					
35379265	11	95	dep	expression	1350:1359	arg1	circulating					1362:1372	circulating	1362:1372	circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice)	1362:1591	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	96	theme	Enterobacteriaceae	1291:1308	arg1	level					1282:1286	a higher level	1273:1286	a higher level of Enterobacteriaceae	1273:1308	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	11	96	theme	Enterobacteriaceae	1291:1308	arg1	expression					1350:1359	intestinal Toll-like receptor-4 (TLR4) expression	1311:1359	intestinal Toll-like receptor-4 (TLR4) expression	1311:1359	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	5	97	theme	stroke	449:454	arg1	patients					456:463	83 stroke patients	446:463	METHODS 83 stroke patients	438:463	METHODS 83 stroke patients were recruited and their cognitive function were measured by Montreal Cognitive Assessment (MoCA) scores 3 months after stroke onset.					
35379265	11	98	theme	stroke	1195:1200	arg1	mice					1202:1205	stroke mice	1195:1205	stroke mice that received microbiota from PSCI patients (PSCI mice)	1195:1261	Consistently, stroke mice that received microbiota from PSCI patients (PSCI mice) presented a higher level of Enterobacteriaceae, intestinal Toll-like receptor-4 (TLR4) expression, circulating LPS, LPS-binding protein (LBP) and inflammatory cytokines, and a lower level of fecal butyrate, severer intestine destruction and cognitive impairment than mice that received microbiota from nPSCI patients (nPSCI mice).					
35379265	7	99	theme	causal	795:800	arg1	relationship					802:813	the causal relationship	791:813	the causal relationship between the gut microbiota and PSCI	791:849	Fecal microbiota transplantation from patients to stroke mice was performed to examine the causal relationship between the gut microbiota and PSCI.					
35379265	9	100	theme	stroke	942:947	arg1	patients					949:956	34 and 49 stroke patients	932:956	34 and 49 stroke patients	932:956	RESULTS 34 and 49 stroke patients were classified as PSCI and non-PSCI, respectively.					
35379265	9	100	theme	stroke	942:947	arg1	non-PSCI					986:993	non-PSCI	986:993	non-PSCI	986:993	RESULTS 34 and 49 stroke patients were classified as PSCI and non-PSCI, respectively.					
35379265	9	100	theme	stroke	942:947	arg1	PSCI					977:980	PSCI	977:980	PSCI	977:980	RESULTS 34 and 49 stroke patients were classified as PSCI and non-PSCI, respectively.					
35660768	6	0	theme	adsorption	1011:1020	arg1	performance					1022:1032	The adsorption performance	1007:1032	The adsorption performance	1007:1032	The adsorption performance was accomplished rapidly, since the adsorption equilibrium was closely gotten within 30 min.					
35660768	1	1	theme	cellulose	269:277	arg1	beads					292:296	cellulose acetate (CA) beads	269:296	cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye	269:352	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	11	2	theme	fast	1899:1902	arg1	removal					1904:1910	the fast removal	1895:1910	the fast removal of toxic cationic dyes	1895:1933	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	3	3	theme	optimal	499:505	arg1	levels					507:512	The optimal levels	495:512	The optimal levels of the most significant identified variables affecting the adsorption process	495:590	The optimal levels of the most significant identified variables affecting the adsorption process were sequential determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs.					
35660768	4	4	theme	pristine	855:862	arg1	CA					864:865	pristine CA	855:865	pristine CA	855:865	The gained results denoted that the surface of SGO@CA beads displayed the higher negative charges (- 42.2 mV) compared to - 35.7 and - 38.7 mV for pristine CA and SGO, respectively.					
35660768	6	5	theme	adsorption	1070:1079	arg1	equilibrium					1081:1091	the adsorption equilibrium	1066:1091	the adsorption equilibrium	1066:1091	The adsorption performance was accomplished rapidly, since the adsorption equilibrium was closely gotten within 30 min.					
35660768	6	5	theme	adsorption	1070:1079	arg1	gotten					1105:1110	gotten	1105:1110	gotten	1105:1110	The adsorption performance was accomplished rapidly, since the adsorption equilibrium was closely gotten within 30 min.					
35660768	10	6	theme	acceptable	1541:1550	arg1	characteristics					1563:1577	acceptable adsorption characteristics	1541:1577	acceptable adsorption characteristics	1541:1577	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	9	7	theme	thermodynamic	1412:1424	arg1	studies					1426:1432	The thermodynamic studies	1408:1432	The thermodynamic studies	1408:1432	The thermodynamic studies designated the spontaneity and endothermic nature of MB dye adsorption.					
35660768	10	8	theme	reuse	1598:1602	arg1	cycles					1604:1609	six successive reuse cycles	1583:1609	six successive reuse cycles	1583:1609	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	11	9	theme	CA	1788:1789	arg1	adsorbents					1880:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	11	9	theme	CA	1788:1789	arg1	beads					1799:1803	the formulated SGO@CA floated beads	1769:1803	the formulated SGO@CA floated beads	1769:1803	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	0	10	theme	surface	136:142	arg1	methodology					144:154	response surface methodology	127:154	response surface methodology	127:154	Sulfonated graphene oxide impregnated cellulose acetate floated beads for adsorption of methylene blue dye: optimization using response surface methodology.					
35660768	11	11	theme	toxic	1915:1919	arg1	dyes					1930:1933	toxic cationic dyes	1915:1933	toxic cationic dyes	1915:1933	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	1	12	theme	graphene	243:250	arg1	oxide					258:262	sulfonated graphene (SGO) oxide	232:262	sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye	232:352	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	11	13	theme	SGO	1784:1786	arg1	adsorbents					1880:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	11	13	theme	SGO	1784:1786	arg1	beads					1799:1803	the formulated SGO@CA floated beads	1769:1803	the formulated SGO@CA floated beads	1769:1803	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	3	14	theme	identified	538:547	arg1	variables					549:557	the most significant identified variables	517:557	the most significant identified variables affecting the adsorption process	517:590	The optimal levels of the most significant identified variables affecting the adsorption process were sequential determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs.					
35660768	9	15	theme	spontaneity	1449:1459	arg1	nature					1477:1482	the spontaneity and endothermic nature	1445:1482	the spontaneity and endothermic nature of MB dye adsorption	1445:1503	The thermodynamic studies designated the spontaneity and endothermic nature of MB dye adsorption.					
35660768	5	16	theme	fast	970:973	arg1	adsorption					975:984	fast adsorption	970:984	fast adsorption	970:984	In addition, the floated SGO@CA beads demonstrated excellent floating property, fast adsorption and easy separation.					
35660768	9	17	theme	endothermic	1465:1475	arg1	nature					1477:1482	the spontaneity and endothermic nature	1445:1482	the spontaneity and endothermic nature of MB dye adsorption	1445:1503	The thermodynamic studies designated the spontaneity and endothermic nature of MB dye adsorption.					
35660768	3	18	theme	adsorption	573:582	arg1	process					584:590	the adsorption process	569:590	the adsorption process	569:590	The optimal levels of the most significant identified variables affecting the adsorption process were sequential determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs.					
35660768	1	19	theme	New	157:159	arg1	beads					186:190	New multi-featured adsorbent beads	157:190	New multi-featured adsorbent beads	157:190	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	1	20	theme	adsorbent	176:184	arg1	beads					186:190	New multi-featured adsorbent beads	157:190	New multi-featured adsorbent beads	157:190	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	2	21	theme	zeta	479:482	arg1	potential					484:492	zeta potential	479:492	zeta potential	479:492	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	7	22	theme	adsorption	1144:1153	arg1	capacity					1155:1162	the adsorption capacity	1140:1162	the adsorption capacity	1140:1162	Furthermore, the adsorption capacity was greatly improved with increasing SGO content from 10 to 30%.					
35660768	0	23	theme	blue	98:101	arg1	dye					103:105	methylene blue dye	88:105	methylene blue dye	88:105	Sulfonated graphene oxide impregnated cellulose acetate floated beads for adsorption of methylene blue dye: optimization using response surface methodology.					
35660768	10	24	theme	Congo	1726:1730	arg1	dyes					1736:1739	anionic Congo red dyes	1718:1739	anionic Congo red dyes	1718:1739	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	8	25	theme	kinetic	1285:1291	arg1	model					1293:1297	the pseudo-second order kinetic model	1261:1297	the pseudo-second order kinetic model	1261:1297	The obtained data were followed the pseudo-second order kinetic model and agreed with Langmuir adsorption isotherm model with a maximum adsorption capacity reached 234.74 mg g-1.					
35660768	2	26	theme	CA	374:375	arg1	beads					387:391	The formulated SGO@CA composite beads	355:391	The formulated SGO@CA composite beads	355:391	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	4	27	theme	gained	712:717	arg1	results					719:725	The gained results	708:725	The gained results denoted that the surface of SGO@CA beads	708:766	The gained results denoted that the surface of SGO@CA beads displayed the higher negative charges (- 42.2 mV) compared to - 35.7 and - 38.7 mV for pristine CA and SGO, respectively.					
35660768	2	28	theme	SGO	370:372	arg1	beads					387:391	The formulated SGO@CA composite beads	355:391	The formulated SGO@CA composite beads	355:391	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	0	29	theme	Sulfonated	0:9	arg1	acetate					48:54	Sulfonated graphene oxide impregnated cellulose acetate	0:54	Sulfonated graphene oxide impregnated cellulose acetate	0:54	Sulfonated graphene oxide impregnated cellulose acetate floated beads for adsorption of methylene blue dye: optimization using response surface methodology.					
35660768	1	30	theme	CA	288:289	arg1	beads					292:296	cellulose acetate (CA) beads	269:296	cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye	269:352	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	0	31	theme	oxide	20:24	arg1	acetate					48:54	Sulfonated graphene oxide impregnated cellulose acetate	0:54	Sulfonated graphene oxide impregnated cellulose acetate	0:54	Sulfonated graphene oxide impregnated cellulose acetate floated beads for adsorption of methylene blue dye: optimization using response surface methodology.					
35660768	11	32	dep	efficient	1841:1849	arg1	easy					1852:1855	easy	1852:1855	easy	1852:1855	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	1	33	dep	blue	340:343	arg1	MB					346:347	MB	346:347	MB	346:347	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	11	34	theme	reusable	1871:1878	arg1	adsorbents					1880:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	11	34	theme	reusable	1871:1878	arg1	beads					1799:1803	the formulated SGO@CA floated beads	1769:1803	the formulated SGO@CA floated beads	1769:1803	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	8	35	theme	adsorption	1324:1333	arg1	model					1344:1348	Langmuir adsorption isotherm model	1315:1348	Langmuir adsorption isotherm model	1315:1348	The obtained data were followed the pseudo-second order kinetic model and agreed with Langmuir adsorption isotherm model with a maximum adsorption capacity reached 234.74 mg g-1.					
35660768	10	36	theme	better	1633:1638	arg1	selectivity					1651:1661	their better adsorption selectivity	1627:1661	their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes	1627:1739	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	0	37	theme	cellulose	38:46	arg1	acetate					48:54	Sulfonated graphene oxide impregnated cellulose acetate	0:54	Sulfonated graphene oxide impregnated cellulose acetate	0:54	Sulfonated graphene oxide impregnated cellulose acetate floated beads for adsorption of methylene blue dye: optimization using response surface methodology.					
35660768	1	38	theme	fast	302:305	arg1	adsorption					307:316	fast adsorption	302:316	fast adsorption of cationic methylene blue (MB) dye	302:352	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	0	39	dep	floated	56:62	arg1	optimization					108:119	optimization	108:119	optimization using response surface methodology	108:154	Sulfonated graphene oxide impregnated cellulose acetate floated beads for adsorption of methylene blue dye: optimization using response surface methodology.					
35660768	4	40	theme	negative	789:796	arg1	charges					798:804	the higher negative charges	778:804	the higher negative charges (- 42.2 mV)	778:816	The gained results denoted that the surface of SGO@CA beads displayed the higher negative charges (- 42.2 mV) compared to - 35.7 and - 38.7 mV for pristine CA and SGO, respectively.					
35660768	4	40	theme	negative	789:796	arg1	42.2 mV					809:815	42.2 mV	809:815	42.2 mV	809:815	The gained results denoted that the surface of SGO@CA beads displayed the higher negative charges (- 42.2 mV) compared to - 35.7 and - 38.7 mV for pristine CA and SGO, respectively.					
35660768	5	41	theme	floating	951:958	arg1	property					960:967	excellent floating property	941:967	excellent floating property	941:967	In addition, the floated SGO@CA beads demonstrated excellent floating property, fast adsorption and easy separation.					
35660768	11	42	theme	dyes	1930:1933	arg1	removal					1904:1910	the fast removal	1895:1910	the fast removal of toxic cationic dyes	1895:1933	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	4	43	theme	@	758:758	arg1	beads					762:766	SGO@CA beads	755:766	SGO@CA beads	755:766	The gained results denoted that the surface of SGO@CA beads displayed the higher negative charges (- 42.2 mV) compared to - 35.7 and - 38.7 mV for pristine CA and SGO, respectively.					
35660768	10	44	theme	MB	1671:1672	arg1	dye					1674:1676	MB dye	1671:1676	MB dye	1671:1676	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	3	45	dep	sequential	597:606	arg1	determined					608:617	determined	608:617	determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs	608:705	The optimal levels of the most significant identified variables affecting the adsorption process were sequential determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs.					
35660768	5	46	theme	SGO	915:917	arg1	beads					922:926	the floated SGO@CA beads	903:926	the floated SGO@CA beads	903:926	In addition, the floated SGO@CA beads demonstrated excellent floating property, fast adsorption and easy separation.					
35660768	3	47	theme	surface	635:641	arg1	RSM					656:658	RSM	656:658	RSM	656:658	The optimal levels of the most significant identified variables affecting the adsorption process were sequential determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs.					
35660768	3	47	theme	surface	635:641	arg1	methodology					643:653	the response surface methodology	622:653	the response surface methodology (RSM)	622:659	The optimal levels of the most significant identified variables affecting the adsorption process were sequential determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs.					
35660768	8	48	theme	adsorption	1365:1374	arg1	capacity					1376:1383	a maximum adsorption capacity	1355:1383	a maximum adsorption capacity reached 234.74 mg g-1	1355:1405	The obtained data were followed the pseudo-second order kinetic model and agreed with Langmuir adsorption isotherm model with a maximum adsorption capacity reached 234.74 mg g-1.					
35660768	5	49	theme	CA	919:920	arg1	beads					922:926	the floated SGO@CA beads	903:926	the floated SGO@CA beads	903:926	In addition, the floated SGO@CA beads demonstrated excellent floating property, fast adsorption and easy separation.					
35660768	7	50	theme	SGO	1201:1203	arg1	content					1205:1211	SGO content	1201:1211	SGO content	1201:1211	Furthermore, the adsorption capacity was greatly improved with increasing SGO content from 10 to 30%.					
35660768	3	51	theme	Plackett-Burman	667:681	arg1	designs					699:705	Plackett-Burman and Box-Behnken designs	667:705	Plackett-Burman and Box-Behnken designs	667:705	The optimal levels of the most significant identified variables affecting the adsorption process were sequential determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs.					
35660768	10	52	theme	cationic	1690:1697	arg1	violet					1707:1712	cationic crystal violet	1690:1712	cationic crystal violet	1690:1712	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	10	53	dep	selectivity	1651:1661	arg1	addition					1615:1622	addition	1615:1622	addition	1615:1622	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	10	54	theme	red	1732:1734	arg1	dyes					1736:1739	anionic Congo red dyes	1718:1739	anionic Congo red dyes	1718:1739	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	1	55	theme	acetate	279:285	arg1	beads					292:296	cellulose acetate (CA) beads	269:296	cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye	269:352	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	11	56	used	used	1814:1817	arg2	beads					1799:1803	the formulated SGO@CA floated beads	1769:1803	the formulated SGO@CA floated beads	1769:1803	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	11	56	used	used	1814:1817	arg2	adsorbents					1880:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	9	57	theme	MB	1487:1488	arg1	adsorption					1494:1503	MB dye adsorption	1487:1503	MB dye adsorption	1487:1503	The thermodynamic studies designated the spontaneity and endothermic nature of MB dye adsorption.					
35660768	10	58	theme	floated	1519:1525	arg1	beads					1527:1531	the floated beads	1515:1531	the floated beads	1515:1531	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	10	59	theme	anionic	1718:1724	arg1	dyes					1736:1739	anionic Congo red dyes	1718:1739	anionic Congo red dyes	1718:1739	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	3	60	theme	Box-Behnken	687:697	arg1	designs					699:705	Plackett-Burman and Box-Behnken designs	667:705	Plackett-Burman and Box-Behnken designs	667:705	The optimal levels of the most significant identified variables affecting the adsorption process were sequential determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs.					
35660768	10	61	theme	adsorption	1552:1561	arg1	characteristics					1563:1577	acceptable adsorption characteristics	1541:1577	acceptable adsorption characteristics	1541:1577	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	11	62	theme	formulated	1773:1782	arg1	adsorbents					1880:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	11	62	theme	formulated	1773:1782	arg1	beads					1799:1803	the formulated SGO@CA floated beads	1769:1803	the formulated SGO@CA floated beads	1769:1803	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	0	63	theme	response	127:134	arg1	methodology					144:154	response surface methodology	127:154	response surface methodology	127:154	Sulfonated graphene oxide impregnated cellulose acetate floated beads for adsorption of methylene blue dye: optimization using response surface methodology.					
35660768	8	64	theme	isotherm	1335:1342	arg1	model					1344:1348	Langmuir adsorption isotherm model	1315:1348	Langmuir adsorption isotherm model	1315:1348	The obtained data were followed the pseudo-second order kinetic model and agreed with Langmuir adsorption isotherm model with a maximum adsorption capacity reached 234.74 mg g-1.					
35660768	9	65	theme	dye	1490:1492	arg1	adsorption					1494:1503	MB dye adsorption	1487:1503	MB dye adsorption	1487:1503	The thermodynamic studies designated the spontaneity and endothermic nature of MB dye adsorption.					
35660768	5	66	theme	easy	990:993	arg1	separation					995:1004	easy separation	990:1004	easy separation	990:1004	In addition, the floated SGO@CA beads demonstrated excellent floating property, fast adsorption and easy separation.					
35660768	3	67	theme	variables	549:557	arg1	levels					507:512	The optimal levels	495:512	The optimal levels of the most significant identified variables affecting the adsorption process	495:590	The optimal levels of the most significant identified variables affecting the adsorption process were sequential determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs.					
35660768	1	68	theme	sulfonated	232:241	arg1	oxide					258:262	sulfonated graphene (SGO) oxide	232:262	sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye	232:352	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	1	69	theme	cationic	321:328	arg1	dye					350:352	cationic methylene blue (MB) dye	321:352	cationic methylene blue (MB) dye	321:352	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	10	70	theme	successive	1587:1596	arg1	cycles					1604:1609	six successive reuse cycles	1583:1609	six successive reuse cycles	1583:1609	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	11	71	theme	floated	1791:1797	arg1	adsorbents					1880:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	11	71	theme	floated	1791:1797	arg1	beads					1799:1803	the formulated SGO@CA floated beads	1769:1803	the formulated SGO@CA floated beads	1769:1803	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	3	72	theme	significant	526:536	arg1	variables					549:557	the most significant identified variables	517:557	the most significant identified variables affecting the adsorption process	517:590	The optimal levels of the most significant identified variables affecting the adsorption process were sequential determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs.					
35660768	11	73	theme	@	1787:1787	arg1	adsorbents					1880:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	11	73	theme	@	1787:1787	arg1	beads					1799:1803	the formulated SGO@CA floated beads	1769:1803	the formulated SGO@CA floated beads	1769:1803	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	5	74	theme	floated	907:913	arg1	beads					922:926	the floated SGO@CA beads	903:926	the floated SGO@CA beads	903:926	In addition, the floated SGO@CA beads demonstrated excellent floating property, fast adsorption and easy separation.					
35660768	8	75	theme	pseudo-second	1265:1277	arg1	model					1293:1297	the pseudo-second order kinetic model	1261:1297	the pseudo-second order kinetic model	1261:1297	The obtained data were followed the pseudo-second order kinetic model and agreed with Langmuir adsorption isotherm model with a maximum adsorption capacity reached 234.74 mg g-1.					
35660768	11	76	theme	cationic	1921:1928	arg1	dyes					1930:1933	toxic cationic dyes	1915:1933	toxic cationic dyes	1915:1933	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	8	77	theme	obtained	1233:1240	arg1	data					1242:1245	The obtained data	1229:1245	The obtained data	1229:1245	The obtained data were followed the pseudo-second order kinetic model and agreed with Langmuir adsorption isotherm model with a maximum adsorption capacity reached 234.74 mg g-1.					
35660768	0	78	theme	methylene	88:96	arg1	dye					103:105	methylene blue dye	88:105	methylene blue dye	88:105	Sulfonated graphene oxide impregnated cellulose acetate floated beads for adsorption of methylene blue dye: optimization using response surface methodology.					
35660768	1	79	theme	blue	340:343	arg1	dye					350:352	cationic methylene blue (MB) dye	321:352	cationic methylene blue (MB) dye	321:352	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	1	80	theme	multi-featured	161:174	arg1	beads					186:190	New multi-featured adsorbent beads	157:190	New multi-featured adsorbent beads	157:190	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	0	81	theme	dye	103:105	arg1	adsorption					74:83	adsorption	74:83	adsorption of methylene blue dye	74:105	Sulfonated graphene oxide impregnated cellulose acetate floated beads for adsorption of methylene blue dye: optimization using response surface methodology.					
35660768	9	82	theme	adsorption	1494:1503	arg1	nature					1477:1482	the spontaneity and endothermic nature	1445:1482	the spontaneity and endothermic nature of MB dye adsorption	1445:1503	The thermodynamic studies designated the spontaneity and endothermic nature of MB dye adsorption.					
35660768	1	83	theme	dye	350:352	arg1	adsorption					307:316	fast adsorption	302:316	fast adsorption of cationic methylene blue (MB) dye	302:352	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	8	84	theme	order	1279:1283	arg1	model					1293:1297	the pseudo-second order kinetic model	1261:1297	the pseudo-second order kinetic model	1261:1297	The obtained data were followed the pseudo-second order kinetic model and agreed with Langmuir adsorption isotherm model with a maximum adsorption capacity reached 234.74 mg g-1.					
35660768	2	85	theme	@	373:373	arg1	beads					387:391	The formulated SGO@CA composite beads	355:391	The formulated SGO@CA composite beads	355:391	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	0	86	theme	graphene	11:18	arg1	acetate					48:54	Sulfonated graphene oxide impregnated cellulose acetate	0:54	Sulfonated graphene oxide impregnated cellulose acetate	0:54	Sulfonated graphene oxide impregnated cellulose acetate floated beads for adsorption of methylene blue dye: optimization using response surface methodology.					
35660768	2	87	theme	several	426:432	arg1	XPS					471:473	XPS	471:473	XPS	471:473	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	2	87	theme	several	426:432	arg1	tools					434:438	several tools	426:438	several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential	426:492	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	2	87	theme	several	426:432	arg1	SEM					461:463	SEM	461:463	SEM	461:463	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	2	87	theme	several	426:432	arg1	potential					484:492	zeta potential	479:492	zeta potential	479:492	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	2	87	theme	several	426:432	arg1	XRD					466:468	XRD	466:468	XRD	466:468	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	2	87	theme	several	426:432	arg1	FTIR					450:453	FTIR	450:453	FTIR	450:453	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	2	87	theme	several	426:432	arg1	TGA					456:458	TGA	456:458	TGA	456:458	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	2	88	theme	formulated	359:368	arg1	beads					387:391	The formulated SGO@CA composite beads	355:391	The formulated SGO@CA composite beads	355:391	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	0	89	theme	impregnated	26:36	arg1	acetate					48:54	Sulfonated graphene oxide impregnated cellulose acetate	0:54	Sulfonated graphene oxide impregnated cellulose acetate	0:54	Sulfonated graphene oxide impregnated cellulose acetate floated beads for adsorption of methylene blue dye: optimization using response surface methodology.					
35660768	4	90	theme	beads	762:766	arg1	surface					744:750	the surface	740:750	the surface of SGO@CA beads	740:766	The gained results denoted that the surface of SGO@CA beads displayed the higher negative charges (- 42.2 mV) compared to - 35.7 and - 38.7 mV for pristine CA and SGO, respectively.					
35660768	10	91	theme	adsorption	1640:1649	arg1	selectivity					1651:1661	their better adsorption selectivity	1627:1661	their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes	1627:1739	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	11	92	theme	separable	1857:1865	arg1	adsorbents					1880:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	11	92	theme	separable	1857:1865	arg1	beads					1799:1803	the formulated SGO@CA floated beads	1769:1803	the formulated SGO@CA floated beads	1769:1803	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	8	93	theme	Langmuir	1315:1322	arg1	adsorption					1324:1333	Langmuir adsorption	1315:1333	Langmuir adsorption isotherm model	1315:1348	The obtained data were followed the pseudo-second order kinetic model and agreed with Langmuir adsorption isotherm model with a maximum adsorption capacity reached 234.74 mg g-1.					
35660768	2	94	theme	composite	377:385	arg1	beads					387:391	The formulated SGO@CA composite beads	355:391	The formulated SGO@CA composite beads	355:391	The formulated SGO@CA composite beads were thoroughly characterized by several tools including FTIR, TGA, SEM, XRD, XPS and zeta potential.					
35660768	5	95	theme	excellent	941:949	arg1	property					960:967	excellent floating property	941:967	excellent floating property	941:967	In addition, the floated SGO@CA beads demonstrated excellent floating property, fast adsorption and easy separation.					
35660768	4	96	theme	higher	782:787	arg1	charges					798:804	the higher negative charges	778:804	the higher negative charges (- 42.2 mV)	778:816	The gained results denoted that the surface of SGO@CA beads displayed the higher negative charges (- 42.2 mV) compared to - 35.7 and - 38.7 mV for pristine CA and SGO, respectively.					
35660768	4	96	theme	higher	782:787	arg1	42.2 mV					809:815	42.2 mV	809:815	42.2 mV	809:815	The gained results denoted that the surface of SGO@CA beads displayed the higher negative charges (- 42.2 mV) compared to - 35.7 and - 38.7 mV for pristine CA and SGO, respectively.					
35660768	4	97	theme	CA	759:760	arg1	beads					762:766	SGO@CA beads	755:766	SGO@CA beads	755:766	The gained results denoted that the surface of SGO@CA beads displayed the higher negative charges (- 42.2 mV) compared to - 35.7 and - 38.7 mV for pristine CA and SGO, respectively.					
35660768	7	98	dep	30	1224:1225	arg1	to					1221:1222	to	1221:1222	to	1221:1222	Furthermore, the adsorption capacity was greatly improved with increasing SGO content from 10 to 30%.					
35660768	3	99	theme	response	626:633	arg1	RSM					656:658	RSM	656:658	RSM	656:658	The optimal levels of the most significant identified variables affecting the adsorption process were sequential determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs.					
35660768	3	99	theme	response	626:633	arg1	methodology					643:653	the response surface methodology	622:653	the response surface methodology (RSM)	622:659	The optimal levels of the most significant identified variables affecting the adsorption process were sequential determined by the response surface methodology (RSM) using Plackett-Burman and Box-Behnken designs.					
35660768	4	100	theme	SGO	755:757	arg1	beads					762:766	SGO@CA beads	755:766	SGO@CA beads	755:766	The gained results denoted that the surface of SGO@CA beads displayed the higher negative charges (- 42.2 mV) compared to - 35.7 and - 38.7 mV for pristine CA and SGO, respectively.					
35660768	11	101	theme	efficient	1841:1849	arg1	adsorbents					1880:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	highly efficient, easy separable and reusable adsorbents	1834:1889	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	11	101	theme	efficient	1841:1849	arg1	beads					1799:1803	the formulated SGO@CA floated beads	1769:1803	the formulated SGO@CA floated beads	1769:1803	These findings assume that the formulated SGO@CA floated beads could be used effectively as highly efficient, easy separable and reusable adsorbents for the fast removal of toxic cationic dyes.					
35660768	1	102	theme	SGO	253:255	arg1	oxide					258:262	sulfonated graphene (SGO) oxide	232:262	sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye	232:352	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
35660768	5	103	theme	@	918:918	arg1	beads					922:926	the floated SGO@CA beads	903:926	the floated SGO@CA beads	903:926	In addition, the floated SGO@CA beads demonstrated excellent floating property, fast adsorption and easy separation.					
35660768	8	104	theme	maximum	1357:1363	arg1	capacity					1376:1383	a maximum adsorption capacity	1355:1383	a maximum adsorption capacity reached 234.74 mg g-1	1355:1405	The obtained data were followed the pseudo-second order kinetic model and agreed with Langmuir adsorption isotherm model with a maximum adsorption capacity reached 234.74 mg g-1.					
35660768	10	105	theme	crystal	1699:1705	arg1	violet					1707:1712	cationic crystal violet	1690:1712	cationic crystal violet	1690:1712	Besides, the floated beads exposed acceptable adsorption characteristics for six successive reuse cycles, in addition to their better adsorption selectivity towards MB dye compared to cationic crystal violet and anionic Congo red dyes.					
35660768	1	106	theme	oxide	258:262	arg1	impregnation					216:227	impregnation	216:227	impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye	216:352	New multi-featured adsorbent beads were fabricated through impregnation of sulfonated graphene (SGO) oxide into cellulose acetate (CA) beads for fast adsorption of cationic methylene blue (MB) dye.					
36549539	7	0	theme	adsorption	1023:1032	arg1	sites					1034:1038	adsorption sites	1023:1038	adsorption sites	1023:1038	suggesting that the BMSAH can provide a larger specific surface area and high number of adsorption sites.					
36549539	10	1	theme	model	1447:1451	arg1	lines					1505:1509	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines	1417:1509	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines	1417:1509	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	8	2	theme	pH	1069:1070	arg1	effects					1045:1051	The effects	1041:1051	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH	1041:1143	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	12	3	theme	hydrogels	2009:2017	arg1	preparation					1908:1918	the preparation	1904:1918	the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions	1904:2058	Our work provides a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions.					
36549539	10	4	theme	pseudo-second-order	1419:1437	arg1	model					1447:1451	A pseudo-second-order kinetic model	1417:1451	A pseudo-second-order kinetic model	1417:1451	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	8	5	theme	ionic	1073:1077	arg1	strength					1079:1086	ionic strength	1073:1086	ionic strength	1073:1086	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	10	6	theme	model	1473:1477	arg1	lines					1505:1509	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines	1417:1509	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines	1417:1509	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	7	7	theme	surface	991:997	arg1	area					999:1002	a larger specific surface area	973:1002	a larger specific surface area	973:1002	suggesting that the BMSAH can provide a larger specific surface area and high number of adsorption sites.					
36549539	2	8	theme	friendly	280:287	arg1	material					336:343	an environmentally friendly, low-cost, biodegradable and easily recyclable material	261:343	an environmentally friendly, low-cost, biodegradable and easily recyclable material	261:343	However, there has been a lack of an environmentally friendly, low-cost, biodegradable and easily recyclable material.					
36549539	9	9	theme	coordination	1314:1325	arg1	interactions					1327:1338	coordination interactions	1314:1338	coordination interactions	1314:1338	XPS and FT-IR analysis showed that Cd2+ was adsorbed mainly via coordination interactions and hydrogen bonds with the carboxyl groups and nitrogen atoms in the BMSAH.					
36549539	10	10	theme	particle	1454:1461	arg1	model					1473:1477	particle diffusion model	1454:1477	particle diffusion model	1454:1477	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	7	11	theme	larger	975:980	arg1	area					999:1002	a larger specific surface area	973:1002	a larger specific surface area	973:1002	suggesting that the BMSAH can provide a larger specific surface area and high number of adsorption sites.					
36549539	8	12	theme	time	1100:1103	arg1	effects					1045:1051	The effects	1041:1051	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH	1041:1143	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	12	13	theme	recyclable	1989:1998	arg1	hydrogels					2009:2017	easily recyclable physical hydrogels	1982:2017	easily recyclable physical hydrogels	1982:2017	Our work provides a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions.					
36549539	0	14	theme	metals	113:118	arg1	ion					128:130	heavy metals cadmium ion	107:130	heavy metals cadmium ion	107:130	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.					
36549539	10	15	theme	intraparticle	1678:1690	arg1	mechanisms					1702:1711	intraparticle diffusion mechanisms	1678:1711	intraparticle diffusion mechanisms	1678:1711	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	5	16	theme	mechanical	762:771	arg1	structure					773:781	the mechanical structure	758:781	the mechanical structure of SA	758:787	Enhance the mechanical structure of SA while making the BM easy to recycle and reuse.					
36549539	6	17	dep	morphology	840:849	arg1	The					836:838	The	836:838	The	836:838	The morphology and structure of the BMSAH were characterized using FT-IR spectroscopy and SEM-EDS.					
36549539	12	18	theme	mechanical	1940:1949	arg1	performance					1951:1961	a low-cost, high mechanical performance	1923:1961	a low-cost, high mechanical performance	1923:1961	Our work provides a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions.					
36549539	10	19	theme	Isothermal	1483:1492	arg1	adsorption					1494:1503	Isothermal adsorption	1483:1503	Isothermal adsorption	1483:1503	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	0	20	theme	ion	128:130	arg1	adsorption					93:102	efficient adsorption	83:102	efficient adsorption of heavy metals cadmium ion	83:130	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.					
36549539	10	21	theme	adsorption	1663:1672	arg1	combination					1640:1650	a combination	1638:1650	a combination	1638:1650	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	10	21	theme	adsorption	1663:1672	arg1	process					1763:1769	an overall chemical-physical adsorption process	1723:1769	an overall chemical-physical adsorption process	1723:1769	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	8	22	theme	different	1179:1187	arg1	conditions					1189:1198	different conditions	1179:1198	different conditions	1179:1198	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	4	23	theme	BM	624:625	arg1	SA					648:649	SA	648:649	SA	648:649	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	4	23	theme	BM	624:625	arg1	alginate					638:645	sodium alginate	631:645	sodium alginate (SA)	631:650	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	4	23	theme	BM	624:625	arg1	combination					609:619	a combination	607:619	a combination of BM	607:625	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	10	24	theme	mechanisms	1702:1711	arg1	combination					1640:1650	a combination	1638:1650	a combination	1638:1650	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	10	24	theme	mechanisms	1702:1711	arg1	process					1763:1769	an overall chemical-physical adsorption process	1723:1769	an overall chemical-physical adsorption process	1723:1769	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	9	25	theme	nitrogen	1388:1395	arg1	atoms					1397:1401	nitrogen atoms	1388:1401	nitrogen atoms in the BMSAH	1388:1414	XPS and FT-IR analysis showed that Cd2+ was adsorbed mainly via coordination interactions and hydrogen bonds with the carboxyl groups and nitrogen atoms in the BMSAH.					
36549539	9	26	with	atoms	1397:1401	arg1	groups					1377:1382	the carboxyl groups	1364:1382	the carboxyl groups	1364:1382	XPS and FT-IR analysis showed that Cd2+ was adsorbed mainly via coordination interactions and hydrogen bonds with the carboxyl groups and nitrogen atoms in the BMSAH.					
36549539	4	27	theme	sodium	631:636	arg1	SA					648:649	SA	648:649	SA	648:649	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	4	27	theme	sodium	631:636	arg1	alginate					638:645	sodium alginate	631:645	sodium alginate (SA)	631:650	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	0	28	theme	waste	63:67	arg1	meal					74:77	rural waste bone meal	57:77	rural waste bone meal	57:77	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.					
36549539	8	29	theme	strong	1213:1218	arg1	capacity					1231:1238	a strong adsorption capacity	1211:1238	a strong adsorption capacity of >90 %	1211:1247	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	12	30	dep	low-cost	1925:1932	arg1	high					1935:1938	high	1935:1938	high	1935:1938	Our work provides a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions.					
36549539	12	31	theme	performance	1951:1961	arg1	preparation					1908:1918	the preparation	1904:1918	the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions	1904:2058	Our work provides a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions.					
36549539	4	32	theme	BM/SA/Ca2+	690:699	arg1	BMSAH					742:746	BMSAH	742:746	BMSAH	742:746	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	4	32	theme	BM/SA/Ca2+	690:699	arg1	hydrogel					732:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel	663:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH)	663:747	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	11	33	theme	adsorption	1789:1798	arg1	capacity					1800:1807	the adsorption capacity	1785:1807	the adsorption capacity of BMSAH	1785:1816	In addition, the adsorption capacity of BMSAH remained above 90 % after three desorption cycles.					
36549539	0	34	theme	efficient	83:91	arg1	adsorption					93:102	efficient adsorption	83:102	efficient adsorption of heavy metals cadmium ion	83:130	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.					
36549539	12	35	theme	new	1889:1891	arg1	method					1893:1898	a new method	1887:1898	a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions	1887:2058	Our work provides a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions.					
36549539	10	36	theme	chemical-physical	1734:1750	arg1	combination					1640:1650	a combination	1638:1650	a combination	1638:1650	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	10	36	theme	chemical-physical	1734:1750	arg1	process					1763:1769	an overall chemical-physical adsorption process	1723:1769	an overall chemical-physical adsorption process	1723:1769	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	4	37	theme	novel	665:669	arg1	BMSAH					742:746	BMSAH	742:746	BMSAH	742:746	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	4	37	theme	novel	665:669	arg1	hydrogel					732:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel	663:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH)	663:747	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	8	38	from	effects	1045:1051	arg1	capacity					1123:1130	the adsorption capacity	1108:1130	the adsorption capacity of the BMSAH	1108:1143	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	4	39	link	cross-linked	709:720	arg1	BMSAH					742:746	BMSAH	742:746	BMSAH	742:746	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	4	39	link	cross-linked	709:720	arg1	hydrogel					732:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel	663:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH)	663:747	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	9	40	with	interactions	1327:1338	arg1	groups					1377:1382	the carboxyl groups	1364:1382	the carboxyl groups	1364:1382	XPS and FT-IR analysis showed that Cd2+ was adsorbed mainly via coordination interactions and hydrogen bonds with the carboxyl groups and nitrogen atoms in the BMSAH.					
36549539	10	41	theme	BMSAH	1544:1548	arg1	surface					1529:1535	the surface	1525:1535	the surface of the BMSAH	1525:1548	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	10	41	theme	BMSAH	1544:1548	arg1	non-uniform					1553:1563	non-uniform	1553:1563	non-uniform	1553:1563	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	12	42	theme	ions	2055:2058	arg1	removal					2032:2038	the removal	2028:2038	the removal of heavy metal ions	2028:2058	Our work provides a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions.					
36549539	3	43	theme	resources	410:418	arg1	waste					401:405	a waste	399:405	a waste of resources	399:418	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	0	44	theme	hydrogel	23:30	arg1	Preparation					0:10	Preparation	0:10	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.	0:131	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.					
36549539	8	45	dep	BMSAH	1201:1205	arg1	has					1207:1209	has	1207:1209	has a strong adsorption capacity of >90 %	1207:1247	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	3	46	theme	bone	456:459	arg1	BM					467:468	BM	467:468	BM	467:468	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	3	46	theme	bone	456:459	arg1	meal					461:464	bone meal	456:464	bone meal (BM)	456:469	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	0	47	theme	sodium	35:40	arg1	alginate					42:49	sodium alginate	35:49	sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion	35:130	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.					
36549539	9	48	from	interactions	1327:1338	arg1	BMSAH					1410:1414	the BMSAH	1406:1414	the BMSAH	1406:1414	XPS and FT-IR analysis showed that Cd2+ was adsorbed mainly via coordination interactions and hydrogen bonds with the carboxyl groups and nitrogen atoms in the BMSAH.					
36549539	4	49	theme	double	702:707	arg1	BMSAH					742:746	BMSAH	742:746	BMSAH	742:746	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	4	49	theme	double	702:707	arg1	hydrogel					732:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel	663:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH)	663:747	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	9	50	theme	XPS	1250:1252	arg1	analysis					1264:1271	XPS and FT-IR analysis	1250:1271	analysis	1264:1271	XPS and FT-IR analysis showed that Cd2+ was adsorbed mainly via coordination interactions and hydrogen bonds with the carboxyl groups and nitrogen atoms in the BMSAH.					
36549539	3	51	used	utilized	381:388	arg2	bones					361:365	bones	361:365	bones	361:365	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	4	52	theme	composite	722:730	arg1	BMSAH					742:746	BMSAH	742:746	BMSAH	742:746	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	4	52	theme	composite	722:730	arg1	hydrogel					732:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel	663:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH)	663:747	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	10	53	theme	ions	1611:1614	arg1	adsorption					1585:1594	the adsorption	1581:1594	the adsorption of heavy metal ions by the BMSAH	1581:1627	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	12	54	theme	physical	2000:2007	arg1	hydrogels					2009:2017	easily recyclable physical hydrogels	1982:2017	easily recyclable physical hydrogels	1982:2017	Our work provides a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions.					
36549539	9	55	theme	FT-IR	1258:1262	arg1	analysis					1264:1271	XPS and FT-IR analysis	1250:1271	analysis	1264:1271	XPS and FT-IR analysis showed that Cd2+ was adsorbed mainly via coordination interactions and hydrogen bonds with the carboxyl groups and nitrogen atoms in the BMSAH.					
36549539	7	56	theme	sites	1034:1038	arg1	area					999:1002	a larger specific surface area	973:1002	a larger specific surface area	973:1002	suggesting that the BMSAH can provide a larger specific surface area and high number of adsorption sites.					
36549539	7	56	theme	sites	1034:1038	arg1	number					1013:1018	high number	1008:1018	high number of adsorption sites	1008:1038	suggesting that the BMSAH can provide a larger specific surface area and high number of adsorption sites.					
36549539	10	57	theme	heavy	1599:1603	arg1	ions					1611:1614	heavy metal ions	1599:1614	heavy metal ions	1599:1614	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	9	58	from	atoms	1397:1401	arg1	BMSAH					1410:1414	the BMSAH	1406:1414	the BMSAH	1406:1414	XPS and FT-IR analysis showed that Cd2+ was adsorbed mainly via coordination interactions and hydrogen bonds with the carboxyl groups and nitrogen atoms in the BMSAH.					
36549539	7	59	theme	high	1008:1011	arg1	number					1013:1018	high number	1008:1018	high number of adsorption sites	1008:1038	suggesting that the BMSAH can provide a larger specific surface area and high number of adsorption sites.					
36549539	3	60	theme	contaminants	490:501	arg1	remediation					475:485	remediation	475:485	remediation of contaminants in water	475:510	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	10	61	theme	kinetic	1439:1445	arg1	model					1447:1451	A pseudo-second-order kinetic model	1417:1451	A pseudo-second-order kinetic model	1417:1451	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	1	62	theme	important	156:164	arg1	method					166:171	an important method	153:171	an important method for removing heavy metals from industrial wastewater	153:224	Adsorption has been an important method for removing heavy metals from industrial wastewater.					
36549539	1	62	theme	important	156:164	arg1	Adsorption					133:142	Adsorption	133:142	Adsorption	133:142	Adsorption has been an important method for removing heavy metals from industrial wastewater.					
36549539	8	63	theme	solution	1060:1067	arg1	pH					1069:1070	the solution pH	1056:1070	the solution pH	1056:1070	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	8	64	theme	adsorption	1112:1121	arg1	capacity					1123:1130	the adsorption capacity	1108:1130	the adsorption capacity of the BMSAH	1108:1143	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	7	65	theme	specific	982:989	arg1	area					999:1002	a larger specific surface area	973:1002	a larger specific surface area	973:1002	suggesting that the BMSAH can provide a larger specific surface area and high number of adsorption sites.					
36549539	10	66	theme	surface	1655:1661	arg1	adsorption					1663:1672	surface adsorption	1655:1672	surface adsorption	1655:1672	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	5	67	theme	SA	786:787	arg1	structure					773:781	the mechanical structure	758:781	the mechanical structure of SA	758:787	Enhance the mechanical structure of SA while making the BM easy to recycle and reuse.					
36549539	6	68	theme	BMSAH	872:876	arg1	morphology					840:849	morphology	840:849	morphology	840:849	The morphology and structure of the BMSAH were characterized using FT-IR spectroscopy and SEM-EDS.					
36549539	6	68	theme	BMSAH	872:876	arg1	structure					855:863	structure	855:863	structure	855:863	The morphology and structure of the BMSAH were characterized using FT-IR spectroscopy and SEM-EDS.					
36549539	10	69	theme	diffusion	1463:1471	arg1	model					1473:1477	particle diffusion model	1454:1477	particle diffusion model	1454:1477	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	0	70	theme	heavy	107:111	arg1	ion					128:130	heavy metals cadmium ion	107:130	heavy metals cadmium ion	107:130	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.					
36549539	8	71	theme	strength	1079:1086	arg1	effects					1045:1051	The effects	1041:1051	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH	1041:1143	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	8	72	theme	BMSAH	1139:1143	arg1	capacity					1123:1130	the adsorption capacity	1108:1130	the adsorption capacity of the BMSAH	1108:1143	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	0	73	theme	cadmium	120:126	arg1	ion					128:130	heavy metals cadmium ion	107:130	heavy metals cadmium ion	107:130	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.					
36549539	10	74	theme	diffusion	1692:1700	arg1	mechanisms					1702:1711	intraparticle diffusion mechanisms	1678:1711	intraparticle diffusion mechanisms	1678:1711	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	9	75	theme	hydrogen	1344:1351	arg1	bonds					1353:1357	hydrogen bonds	1344:1357	hydrogen bonds	1344:1357	XPS and FT-IR analysis showed that Cd2+ was adsorbed mainly via coordination interactions and hydrogen bonds with the carboxyl groups and nitrogen atoms in the BMSAH.					
36549539	8	76	theme	contact	1092:1098	arg1	time					1100:1103	contact time	1092:1103	contact time	1092:1103	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	10	77	theme	adsorption	1494:1503	arg1	lines					1505:1509	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines	1417:1509	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines	1417:1509	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	3	78	theme	promising	528:536	arg1	alternative					538:548	a promising alternative	526:548	a promising alternative for resource utilization of bones	526:582	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	3	79	from	water	506:510	arg1	remediation					475:485	remediation	475:485	remediation of contaminants in water	475:510	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	3	80	theme	bones	578:582	arg1	utilization					563:573	resource utilization	554:573	resource utilization of bones	554:582	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	9	81	theme	carboxyl	1368:1375	arg1	groups					1377:1382	the carboxyl groups	1364:1382	the carboxyl groups	1364:1382	XPS and FT-IR analysis showed that Cd2+ was adsorbed mainly via coordination interactions and hydrogen bonds with the carboxyl groups and nitrogen atoms in the BMSAH.					
36549539	4	82	theme	ion	685:687	arg1	BMSAH					742:746	BMSAH	742:746	BMSAH	742:746	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	4	82	theme	ion	685:687	arg1	hydrogel					732:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel	663:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH)	663:747	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	2	83	dep	friendly	280:287	arg1	biodegradable					300:312	biodegradable	300:312	biodegradable	300:312	However, there has been a lack of an environmentally friendly, low-cost, biodegradable and easily recyclable material.					
36549539	2	83	dep	friendly	280:287	arg1	recyclable					325:334	recyclable	325:334	recyclable	325:334	However, there has been a lack of an environmentally friendly, low-cost, biodegradable and easily recyclable material.					
36549539	2	83	dep	friendly	280:287	arg1	low-cost					290:297	low-cost	290:297	low-cost	290:297	However, there has been a lack of an environmentally friendly, low-cost, biodegradable and easily recyclable material.					
36549539	0	84	theme	rural	57:61	arg1	meal					74:77	rural waste bone meal	57:77	rural waste bone meal	57:77	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.					
36549539	11	85	theme	desorption	1850:1859	arg1	cycles					1861:1866	three desorption cycles	1844:1866	three desorption cycles	1844:1866	In addition, the adsorption capacity of BMSAH remained above 90 % after three desorption cycles.					
36549539	3	86	from	remediation	475:485	arg1	water					506:510	water	506:510	water	506:510	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	0	87	theme	bone	69:72	arg1	meal					74:77	rural waste bone meal	57:77	rural waste bone meal	57:77	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.					
36549539	6	88	theme	FT-IR	903:907	arg1	spectroscopy					909:920	FT-IR spectroscopy	903:920	FT-IR spectroscopy	903:920	The morphology and structure of the BMSAH were characterized using FT-IR spectroscopy and SEM-EDS.					
36549539	12	89	theme	low-cost	1925:1932	arg1	performance					1951:1961	a low-cost, high mechanical performance	1923:1961	a low-cost, high mechanical performance	1923:1961	Our work provides a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions.					
36549539	3	90	from	contaminants	490:501	arg1	water					506:510	water	506:510	water	506:510	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	4	91	theme	BM/SA/calcium	671:683	arg1	BMSAH					742:746	BMSAH	742:746	BMSAH	742:746	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	4	91	theme	BM/SA/calcium	671:683	arg1	hydrogel					732:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel	663:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH)	663:747	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	12	92	theme	biodegradable	1964:1976	arg1	preparation					1908:1918	the preparation	1904:1918	the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions	1904:2058	Our work provides a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions.					
36549539	8	93	theme	adsorption	1220:1229	arg1	capacity					1231:1238	a strong adsorption capacity	1211:1238	a strong adsorption capacity of >90 %	1211:1247	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	0	94	theme	novel	17:21	arg1	hydrogel					23:30	a novel hydrogel	15:30	a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion	15:130	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.					
36549539	12	95	theme	heavy	2043:2047	arg1	ions					2055:2058	heavy metal ions	2043:2058	heavy metal ions	2043:2058	Our work provides a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions.					
36549539	9	96	with	bonds	1353:1357	arg1	groups					1377:1382	the carboxyl groups	1364:1382	the carboxyl groups	1364:1382	XPS and FT-IR analysis showed that Cd2+ was adsorbed mainly via coordination interactions and hydrogen bonds with the carboxyl groups and nitrogen atoms in the BMSAH.					
36549539	10	97	theme	adsorption	1752:1761	arg1	combination					1640:1650	a combination	1638:1650	a combination	1638:1650	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	10	97	theme	adsorption	1752:1761	arg1	process					1763:1769	an overall chemical-physical adsorption process	1723:1769	an overall chemical-physical adsorption process	1723:1769	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	8	98	theme	%	1247:1247	arg1	capacity					1231:1238	a strong adsorption capacity	1211:1238	a strong adsorption capacity of >90 %	1211:1247	The effects of the solution pH, ionic strength and contact time on the adsorption capacity of the BMSAH were investigated in depth, Under different conditions, BMSAH has a strong adsorption capacity of >90 %.					
36549539	3	99	theme	efficient	431:439	arg1	application					441:451	efficient application	431:451	efficient application of bone meal (BM) for remediation of contaminants in water	431:510	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	1	100	theme	heavy	186:190	arg1	metals					192:197	heavy metals	186:197	heavy metals	186:197	Adsorption has been an important method for removing heavy metals from industrial wastewater.					
36549539	10	101	theme	overall	1726:1732	arg1	combination					1640:1650	a combination	1638:1650	a combination	1638:1650	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	10	101	theme	overall	1726:1732	arg1	process					1763:1769	an overall chemical-physical adsorption process	1723:1769	an overall chemical-physical adsorption process	1723:1769	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	12	102	theme	metal	2049:2053	arg1	ions					2055:2058	heavy metal ions	2043:2058	heavy metal ions	2043:2058	Our work provides a new method for the preparation of a low-cost, high mechanical performance, biodegradable and easily recyclable physical hydrogels used for the removal of heavy metal ions.					
36549539	11	103	theme	BMSAH	1812:1816	arg1	capacity					1800:1807	the adsorption capacity	1785:1807	the adsorption capacity of BMSAH	1785:1816	In addition, the adsorption capacity of BMSAH remained above 90 % after three desorption cycles.					
36549539	0	104	theme	alginate	42:49	arg1	hydrogel					23:30	a novel hydrogel	15:30	a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion	15:130	Preparation of a novel hydrogel of sodium alginate using rural waste bone meal for efficient adsorption of heavy metals cadmium ion.					
36549539	9	105	from	bonds	1353:1357	arg1	BMSAH					1410:1414	the BMSAH	1406:1414	the BMSAH	1406:1414	XPS and FT-IR analysis showed that Cd2+ was adsorbed mainly via coordination interactions and hydrogen bonds with the carboxyl groups and nitrogen atoms in the BMSAH.					
36549539	1	106	attach	removing	177:184	arg1	wastewater					215:224	industrial wastewater	204:224	industrial wastewater	204:224	Adsorption has been an important method for removing heavy metals from industrial wastewater.					
36549539	1	106	attach	removing	177:184	arg2	Adsorption					133:142	Adsorption	133:142	Adsorption	133:142	Adsorption has been an important method for removing heavy metals from industrial wastewater.					
36549539	1	106	attach	removing	177:184	arg2	method					166:171	an important method	153:171	an important method for removing heavy metals from industrial wastewater	153:224	Adsorption has been an important method for removing heavy metals from industrial wastewater.					
36549539	3	107	theme	meal	461:464	arg1	application					441:451	efficient application	431:451	efficient application of bone meal (BM) for remediation of contaminants in water	431:510	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	3	108	theme	resource	554:561	arg1	utilization					563:573	resource utilization	554:573	resource utilization of bones	554:582	China produces bones are not fully utilized leads to a waste of resources Therefore, efficient application of bone meal (BM) for remediation of contaminants in water would provide a promising alternative for resource utilization of bones.					
36549539	10	109	theme	metal	1605:1609	arg1	ions					1611:1614	heavy metal ions	1599:1614	heavy metal ions	1599:1614	A pseudo-second-order kinetic model, particle diffusion model and Isothermal adsorption lines indicate that the surface of the BMSAH is non-uniform suggesting that the adsorption of heavy metal ions by the BMSAH involves a combination of surface adsorption and intraparticle diffusion mechanisms, which is an overall chemical-physical adsorption process.					
36549539	1	110	theme	industrial	204:213	arg1	wastewater					215:224	industrial wastewater	204:224	industrial wastewater	204:224	Adsorption has been an important method for removing heavy metals from industrial wastewater.					
36549539	4	111	theme	cross-linked	709:720	arg1	BMSAH					742:746	BMSAH	742:746	BMSAH	742:746	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	4	111	theme	cross-linked	709:720	arg1	hydrogel					732:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel	663:739	a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH)	663:747	In this paper, we use a combination of BM and sodium alginate (SA) to prepare a novel BM/SA/calcium ion (BM/SA/Ca2+) double cross-linked composite hydrogel (BMSAH).					
36549539	2	112	theme	material	336:343	arg1	lack					253:256	a lack	251:256	a lack of an environmentally friendly, low-cost, biodegradable and easily recyclable material	251:343	However, there has been a lack of an environmentally friendly, low-cost, biodegradable and easily recyclable material.					
35044402	4	0	theme	PCO	772:774	arg1	efficiency					758:767	higher encapsulation efficiency	737:767	higher encapsulation efficiency of PCO	737:774	NE-PCO-SA showed a smaller particle size and higher encapsulation efficiency of PCO than ME-PCO-SA (0.16 μm vs. 3.5 μm; 98.1% vs. 92.0%).					
35044402	4	0	theme	PCO	772:774	arg1	size					728:731	a smaller particle size	709:731	a smaller particle size	709:731	NE-PCO-SA showed a smaller particle size and higher encapsulation efficiency of PCO than ME-PCO-SA (0.16 μm vs. 3.5 μm; 98.1% vs. 92.0%).					
35044402	2	1	theme	work	284:287	arg1	aim					272:274	The aim	268:274	The aim of this work	268:287	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	1	2	theme	high	224:227	arg1	content					244:250	its high linolenic acid content	220:250	its high linolenic acid content (ALA, 18:3 ω3)	220:265	Among vegetable oils, chia oil has been gaining interest in recent years due to its high linolenic acid content (ALA, 18:3 ω3).					
35044402	8	3	dep	NE-PCO-SA	1732:1740	arg1	%					1767:1767	61.8%	1763:1767	61.8%	1763:1767	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	8	3	dep	NE-PCO-SA	1732:1740	arg1	%					1747:1747	58.8%	1743:1747	58.8%	1743:1747	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	7	4	theme	surface	1559:1565	arg1	area					1567:1570	their greater surface area	1545:1570	their greater surface area	1545:1570	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	6	5	theme	PCO	1101:1103	arg1	release					1105:1111	the PCO release	1097:1111	the PCO release from ME-PCO-SA and NE-PCO-SA	1097:1140	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	3	6	theme	micro-	456:461	arg1	particles					478:486	PCO micro- and nano-sized particles	452:486	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA)	452:564	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	1	7	theme	acid	239:242	arg1	content					244:250	its high linolenic acid content	220:250	its high linolenic acid content (ALA, 18:3 ω3)	220:265	Among vegetable oils, chia oil has been gaining interest in recent years due to its high linolenic acid content (ALA, 18:3 ω3).					
35044402	7	8	theme	PCO	1315:1317	arg1	release					1319:1325	a significantly higher PCO release	1292:1325	a significantly higher PCO release	1292:1325	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	4	9	theme	higher	737:742	arg1	efficiency					758:767	higher encapsulation efficiency	737:767	higher encapsulation efficiency of PCO	737:774	NE-PCO-SA showed a smaller particle size and higher encapsulation efficiency of PCO than ME-PCO-SA (0.16 μm vs. 3.5 μm; 98.1% vs. 92.0%).					
35044402	6	10	theme	sodium	1210:1215	arg1	alginate					1217:1224	sodium alginate	1210:1224	sodium alginate	1210:1224	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	1	11	theme	chia	162:165	arg1	oil					167:169	chia oil	162:169	chia oil	162:169	Among vegetable oils, chia oil has been gaining interest in recent years due to its high linolenic acid content (ALA, 18:3 ω3).					
35044402	3	12	theme	central	637:643	arg1	point					665:669	a central composite plus star point	635:669	a central composite plus star point experimental design	635:689	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	8	13	theme	PCO	1646:1648	arg1	NE-PCO-SA					1732:1740	NE-PCO-SA	1732:1740	NE-PCO-SA	1732:1740	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	8	13	theme	PCO	1646:1648	arg1	similar					1723:1729	similar	1723:1729	similar	1723:1729	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	8	13	theme	PCO	1646:1648	arg1	release					1650:1656	the PCO release	1642:1656	the PCO release from both particle systems after the digestion of the matrix	1642:1717	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	7	14	dep	NE-PCO-SA	1353:1361	arg1	%					1368:1368	78.4%	1364:1368	78.4%	1364:1368	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	15	theme	free	1465:1468	arg1	ω-3					1497:1499	C18:3 ω-3	1491:1499	C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%)	1491:1535	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	15	theme	free	1465:1468	arg1	acids					1476:1480	individual free fatty acids	1454:1480	individual free fatty acids	1454:1480	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	4	16	theme	μm	808:809	arg1	%					816:816	0.16 μm vs. 3.5 μm; 98.1%	792:816	%	816:816	NE-PCO-SA showed a smaller particle size and higher encapsulation efficiency of PCO than ME-PCO-SA (0.16 μm vs. 3.5 μm; 98.1% vs. 92.0%).					
35044402	6	17	theme	oil	997:999	arg1	release					1001:1007	sustained oil release	987:1007	sustained oil release	987:1007	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	6	18	theme	intestinal	1165:1174	arg1	phase					1176:1180	the intestinal phase	1161:1180	the intestinal phase	1161:1180	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	3	19	theme	nano-sized	467:476	arg1	particles					478:486	PCO micro- and nano-sized particles	452:486	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA)	452:564	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	0	20	theme	gastrointestinal	112:127	arg1	digestion					129:137	in vitro gastrointestinal digestion	103:137	in vitro gastrointestinal digestion	103:137	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.					
35044402	5	21	theme	in	918:919	arg1	digestion					951:959	in vitro static gastrointestinal digestion	918:959	in vitro static gastrointestinal digestion	918:959	Emulsions (NE-PCO and ME-PCO) and particles (NE-PCO-SA and ME-PCO-SA) were subjected to in vitro static gastrointestinal digestion.					
35044402	0	22	theme	particle	17:24	arg1	size					26:29	the particle size	13:29	the particle size of encapsulated chia oil	13:54	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.					
35044402	1	23	from	interest	188:195	arg1	years					207:211	recent years	200:211	recent years	200:211	Among vegetable oils, chia oil has been gaining interest in recent years due to its high linolenic acid content (ALA, 18:3 ω3).					
35044402	9	24	theme	spray-dried	1910:1920	arg1	micro					1922:1926	Sodium alginate spray-dried micro	1894:1926	Sodium alginate spray-dried micro	1894:1926	Sodium alginate spray-dried micro and nanoparticles showed great potential for vehiculation of omega-3 rich oils in the design of functional foods.					
35044402	7	25	theme	higher	1427:1432	arg1	bioaccessibility					1434:1449	also higher bioaccessibility	1422:1449	also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area	1422:1570	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	5	26	dep	Emulsions	830:838	arg1	ME-PCO					852:857	ME-PCO	852:857	ME-PCO	852:857	Emulsions (NE-PCO and ME-PCO) and particles (NE-PCO-SA and ME-PCO-SA) were subjected to in vitro static gastrointestinal digestion.					
35044402	5	26	dep	Emulsions	830:838	arg1	NE-PCO					841:846	NE-PCO	841:846	NE-PCO	841:846	Emulsions (NE-PCO and ME-PCO) and particles (NE-PCO-SA and ME-PCO-SA) were subjected to in vitro static gastrointestinal digestion.					
35044402	5	26	dep	Emulsions	830:838	arg1	Emulsions					830:838	Emulsions	830:838	Emulsions (NE-PCO and ME-PCO)	830:858	Emulsions (NE-PCO and ME-PCO) and particles (NE-PCO-SA and ME-PCO-SA) were subjected to in vitro static gastrointestinal digestion.					
35044402	3	27	theme	plus	655:658	arg1	point					665:669	a central composite plus star point	635:669	a central composite plus star point experimental design	635:689	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	3	28	theme	encapsulating	520:532	arg1	agent					534:538	an encapsulating agent	517:538	an encapsulating agent (ME-PCO-SA and NE-PCO-SA)	517:564	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	2	29	theme	PCO	413:415	arg1	release					417:423	PCO release	413:423	PCO release	413:423	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	6	30	theme	alginate	1217:1224	arg1	suitability					1195:1205	the suitability	1191:1205	the suitability of sodium alginate as an intestine-site release polymer	1191:1261	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	9	31	theme	great	1953:1957	arg1	potential					1959:1967	great potential	1953:1967	great potential for vehiculation of omega-3 rich oils	1953:2005	Sodium alginate spray-dried micro and nanoparticles showed great potential for vehiculation of omega-3 rich oils in the design of functional foods.					
35044402	0	32	dep	in	103:104	arg1	vitro					106:110	vitro	106:110	vitro	106:110	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.					
35044402	0	33	from	Influence	0:8	arg1	bioaccessibility					79:94	bioaccessibility	79:94	bioaccessibility	79:94	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.					
35044402	0	33	from	Influence	0:8	arg1	release					67:73	oil release	63:73	oil release	63:73	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.					
35044402	5	34	theme	static	927:932	arg1	digestion					951:959	in vitro static gastrointestinal digestion	918:959	in vitro static gastrointestinal digestion	918:959	Emulsions (NE-PCO and ME-PCO) and particles (NE-PCO-SA and ME-PCO-SA) were subjected to in vitro static gastrointestinal digestion.					
35044402	3	35	dep	point	665:669	arg1	design					684:689	experimental design	671:689	a central composite plus star point experimental design	635:689	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	0	36	dep	release	67:73	arg1	the					59:61	the	59:61	the	59:61	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.					
35044402	9	37	theme	rich	1997:2000	arg1	oils					2002:2005	omega-3 rich oils	1989:2005	omega-3 rich oils	1989:2005	Sodium alginate spray-dried micro and nanoparticles showed great potential for vehiculation of omega-3 rich oils in the design of functional foods.					
35044402	8	38	dep	similar	1723:1729	arg1	NE-PCO-SA					1732:1740	NE-PCO-SA	1732:1740	NE-PCO-SA	1732:1740	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	8	38	dep	similar	1723:1729	arg1	similar					1723:1729	similar	1723:1729	similar	1723:1729	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	8	38	dep	similar	1723:1729	arg1	release					1650:1656	the PCO release	1642:1656	the PCO release from both particle systems after the digestion of the matrix	1642:1717	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	2	39	theme	chia	362:365	arg1	oil					367:369	encapsulated purified chia oil	340:369	encapsulated purified chia oil (PCO)	340:375	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	2	39	theme	chia	362:365	arg1	PCO					372:374	PCO	372:374	PCO	372:374	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	2	40	theme	encapsulated	340:351	arg1	oil					367:369	encapsulated purified chia oil	340:369	encapsulated purified chia oil (PCO)	340:375	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	2	40	theme	encapsulated	340:351	arg1	PCO					372:374	PCO	372:374	PCO	372:374	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	7	41	dep	in	1333:1334	arg1	vitro					1336:1340	vitro	1336:1340	vitro	1336:1340	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	42	theme	fatty	1470:1474	arg1	ω-3					1497:1499	C18:3 ω-3	1491:1499	C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%)	1491:1535	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	42	theme	fatty	1470:1474	arg1	acids					1476:1480	individual free fatty acids	1454:1480	individual free fatty acids	1454:1480	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	4	43	theme	smaller	711:717	arg1	size					728:731	a smaller particle size	709:731	a smaller particle size	709:731	NE-PCO-SA showed a smaller particle size and higher encapsulation efficiency of PCO than ME-PCO-SA (0.16 μm vs. 3.5 μm; 98.1% vs. 92.0%).					
35044402	2	44	theme	size	332:335	arg1	influence					306:314	the influence	302:314	the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion	302:449	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	6	45	theme	release	1247:1253	arg1	polymer					1255:1261	an intestine-site release polymer	1229:1261	an intestine-site release polymer	1229:1261	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	6	46	from	NE-PCO-SA	1132:1140	arg1	release					1105:1111	the PCO release	1097:1111	the PCO release from ME-PCO-SA and NE-PCO-SA	1097:1140	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	0	47	theme	size	26:29	arg1	Influence					0:8	Influence	0:8	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.	0:138	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.					
35044402	2	48	from	influence	306:314	arg1	efficiency					398:407	encapsulation efficiency	384:407	encapsulation efficiency	384:407	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	2	48	from	influence	306:314	arg1	release					417:423	PCO release	413:423	PCO release	413:423	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	0	49	theme	oil	52:54	arg1	size					26:29	the particle size	13:29	the particle size of encapsulated chia oil	13:54	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.					
35044402	9	50	theme	functional	2024:2033	arg1	foods					2035:2039	functional foods	2024:2039	functional foods	2024:2039	Sodium alginate spray-dried micro and nanoparticles showed great potential for vehiculation of omega-3 rich oils in the design of functional foods.					
35044402	0	51	theme	encapsulated	34:45	arg1	oil					52:54	encapsulated chia oil	34:54	encapsulated chia oil	34:54	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.					
35044402	9	52	theme	alginate	1901:1908	arg1	micro					1922:1926	Sodium alginate spray-dried micro	1894:1926	Sodium alginate spray-dried micro	1894:1926	Sodium alginate spray-dried micro and nanoparticles showed great potential for vehiculation of omega-3 rich oils in the design of functional foods.					
35044402	1	53	theme	recent	200:205	arg1	years					207:211	recent years	200:211	recent years	200:211	Among vegetable oils, chia oil has been gaining interest in recent years due to its high linolenic acid content (ALA, 18:3 ω3).					
35044402	8	54	theme	PCO	1881:1883	arg1	release					1885:1891	the PCO release	1877:1891	the PCO release	1877:1891	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	2	55	dep	in	432:433	arg1	vitro					435:439	vitro	435:439	vitro	435:439	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	8	56	theme	partial	1837:1843	arg1	gelation					1851:1858	partial ionic gelation	1837:1858	partial ionic gelation of SA	1837:1864	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	6	57	dep	showed	980:985	arg1	whereas					1089:1095	whereas	1089:1095	whereas	1089:1095	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	6	58	from	ME-PCO-SA	1118:1126	arg1	release					1105:1111	the PCO release	1097:1111	the PCO release from ME-PCO-SA and NE-PCO-SA	1097:1140	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	0	59	theme	chia	47:50	arg1	oil					52:54	encapsulated chia oil	34:54	encapsulated chia oil	34:54	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.					
35044402	7	60	theme	in	1333:1334	arg1	NE-PCO-SA					1353:1361	NE-PCO-SA	1353:1361	NE-PCO-SA	1353:1361	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	60	theme	in	1333:1334	arg1	digestion					1342:1350	in vitro digestion	1333:1350	in vitro digestion (NE-PCO-SA, 78.4%)	1333:1369	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	3	61	theme	nano	593:596	arg1	spray-drying					598:609	micro and nano spray-drying	583:609	spray-drying	598:609	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	7	62	theme	greater	1551:1557	arg1	area					1567:1570	their greater surface area	1545:1570	their greater surface area	1545:1570	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	8	63	theme	SA	1863:1864	arg1	gelation					1851:1858	partial ionic gelation	1837:1858	partial ionic gelation of SA	1837:1864	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	3	64	theme	micro	583:587	arg1	spray-drying					598:609	micro and nano spray-drying	583:609	spray-drying	598:609	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	1	65	theme	linolenic	229:237	arg1	acid					239:242	linolenic acid	229:242	its high linolenic acid content (ALA, 18:3 ω3)	220:265	Among vegetable oils, chia oil has been gaining interest in recent years due to its high linolenic acid content (ALA, 18:3 ω3).					
35044402	7	66	theme	higher	1308:1313	arg1	release					1319:1325	a significantly higher PCO release	1292:1325	a significantly higher PCO release	1292:1325	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	67	theme	Nano-sized	1264:1273	arg1	particles					1275:1283	Nano-sized particles	1264:1283	Nano-sized particles	1264:1283	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	68	theme	individual	1454:1463	arg1	ω-3					1497:1499	C18:3 ω-3	1491:1499	C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%)	1491:1535	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	68	theme	individual	1454:1463	arg1	acids					1476:1480	individual free fatty acids	1454:1480	individual free fatty acids	1454:1480	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	4	69	theme	encapsulation	744:756	arg1	efficiency					758:767	higher encapsulation efficiency	737:767	higher encapsulation efficiency of PCO	737:774	NE-PCO-SA showed a smaller particle size and higher encapsulation efficiency of PCO than ME-PCO-SA (0.16 μm vs. 3.5 μm; 98.1% vs. 92.0%).					
35044402	5	70	dep	particles	864:872	arg1	particles					864:872	particles	864:872	particles (NE-PCO-SA and ME-PCO-SA)	864:898	Emulsions (NE-PCO and ME-PCO) and particles (NE-PCO-SA and ME-PCO-SA) were subjected to in vitro static gastrointestinal digestion.					
35044402	5	70	dep	particles	864:872	arg1	ME-PCO-SA					889:897	ME-PCO-SA	889:897	ME-PCO-SA	889:897	Emulsions (NE-PCO and ME-PCO) and particles (NE-PCO-SA and ME-PCO-SA) were subjected to in vitro static gastrointestinal digestion.					
35044402	5	70	dep	particles	864:872	arg1	NE-PCO-SA					875:883	NE-PCO-SA	875:883	NE-PCO-SA	875:883	Emulsions (NE-PCO and ME-PCO) and particles (NE-PCO-SA and ME-PCO-SA) were subjected to in vitro static gastrointestinal digestion.					
35044402	3	71	theme	composite	645:653	arg1	point					665:669	a central composite plus star point	635:669	a central composite plus star point experimental design	635:689	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	6	72	theme	sustained	987:995	arg1	release					1001:1007	sustained oil release	987:1007	sustained oil release	987:1007	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	6	73	theme	intestinal	1069:1078	arg1	digestion					1040:1048	digestion	1040:1048	digestion (oral, gastric and intestinal phases)	1040:1086	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	6	73	theme	intestinal	1069:1078	arg1	phases					1080:1085	oral, gastric and intestinal phases	1051:1085	oral, gastric and intestinal phases	1051:1085	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	3	74	theme	experimental	671:682	arg1	design					684:689	experimental design	671:689	a central composite plus star point experimental design	635:689	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	3	75	theme	sodium	493:498	arg1	SA					510:511	SA	510:511	SA	510:511	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	3	75	theme	sodium	493:498	arg1	alginate					500:507	sodium alginate	493:507	sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA)	493:564	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	6	76	theme	gastric	1057:1063	arg1	digestion					1040:1048	digestion	1040:1048	digestion (oral, gastric and intestinal phases)	1040:1086	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	6	76	theme	gastric	1057:1063	arg1	phases					1080:1085	oral, gastric and intestinal phases	1051:1085	oral, gastric and intestinal phases	1051:1085	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	3	77	theme	star	660:663	arg1	point					665:669	a central composite plus star point	635:669	a central composite plus star point experimental design	635:689	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	7	78	dep	particles	1388:1396	arg1	ME-PCO-SA					1399:1407	ME-PCO-SA	1399:1407	ME-PCO-SA	1399:1407	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	78	dep	particles	1388:1396	arg1	%					1414:1414	69.8%	1410:1414	69.8%	1410:1414	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	8	79	dep	%	1747:1747	arg1	ME-PCO-SA-Y					1750:1760	ME-PCO-SA-Y	1750:1760	ME-PCO-SA-Y	1750:1760	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	6	80	theme	oral	1051:1054	arg1	digestion					1040:1048	digestion	1040:1048	digestion (oral, gastric and intestinal phases)	1040:1086	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	6	80	theme	oral	1051:1054	arg1	phases					1080:1085	oral, gastric and intestinal phases	1051:1085	oral, gastric and intestinal phases	1051:1085	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	8	81	theme	matrix	1712:1717	arg1	digestion					1695:1703	the digestion	1691:1703	the digestion of the matrix	1691:1717	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	6	82	theme	digestion	1040:1048	arg1	phases					1030:1035	the three phases	1020:1035	the three phases of digestion (oral, gastric and intestinal phases)	1020:1086	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	3	83	dep	agent	534:538	arg1	ME-PCO-SA					541:549	ME-PCO-SA	541:549	ME-PCO-SA	541:549	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	3	83	dep	agent	534:538	arg1	NE-PCO-SA					555:563	NE-PCO-SA	555:563	NE-PCO-SA	555:563	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	0	84	theme	oil	63:65	arg1	release					67:73	oil release	63:73	oil release	63:73	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.					
35044402	1	85	theme	vegetable	146:154	arg1	oils					156:159	vegetable oils	146:159	vegetable oils	146:159	Among vegetable oils, chia oil has been gaining interest in recent years due to its high linolenic acid content (ALA, 18:3 ω3).					
35044402	2	86	theme	encapsulation	384:396	arg1	efficiency					398:407	encapsulation efficiency	384:407	encapsulation efficiency	384:407	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	1	87	dep	content	244:250	arg1	ALA					253:255	ALA	253:255	ALA	253:255	Among vegetable oils, chia oil has been gaining interest in recent years due to its high linolenic acid content (ALA, 18:3 ω3).					
35044402	1	87	dep	content	244:250	arg1	18:3					258:261	18:3 ω3	258:264	18:3 ω3	258:264	Among vegetable oils, chia oil has been gaining interest in recent years due to its high linolenic acid content (ALA, 18:3 ω3).					
35044402	7	88	theme	micro-sized	1376:1386	arg1	particles					1388:1396	micro-sized particles	1376:1396	micro-sized particles (ME-PCO-SA, 69.8%)	1376:1415	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	2	89	theme	in	432:433	arg1	digestion					441:449	in vitro digestion	432:449	in vitro digestion	432:449	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	5	90	theme	gastrointestinal	934:949	arg1	digestion					951:959	in vitro static gastrointestinal digestion	918:959	in vitro static gastrointestinal digestion	918:959	Emulsions (NE-PCO and ME-PCO) and particles (NE-PCO-SA and ME-PCO-SA) were subjected to in vitro static gastrointestinal digestion.					
35044402	7	91	dep	NE-PCO-SA	1502:1510	arg1	ME-PCO-SA					1520:1528	ME-PCO-SA	1520:1528	ME-PCO-SA	1520:1528	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	91	dep	NE-PCO-SA	1502:1510	arg1	%					1534:1534	7.9%	1531:1534	7.9%	1531:1534	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	91	dep	NE-PCO-SA	1502:1510	arg1	%					1517:1517	23.6%	1513:1517	23.6%	1513:1517	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	92	theme	C18:3	1491:1495	arg1	NE-PCO-SA					1502:1510	NE-PCO-SA	1502:1510	NE-PCO-SA	1502:1510	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	92	theme	C18:3	1491:1495	arg1	ω-3					1497:1499	C18:3 ω-3	1491:1499	C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%)	1491:1535	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	8	93	theme	calcium	1792:1798	arg1	ions					1800:1803	the calcium ions	1788:1803	the calcium ions contained in the yogurt	1788:1827	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	2	94	theme	oil	367:369	arg1	size					332:335	the particle size	319:335	the particle size of encapsulated purified chia oil (PCO)	319:375	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	9	95	theme	oils	2002:2005	arg1	potential					1959:1967	great potential	1953:1967	great potential for vehiculation of omega-3 rich oils	1953:2005	Sodium alginate spray-dried micro and nanoparticles showed great potential for vehiculation of omega-3 rich oils in the design of functional foods.					
35044402	3	96	with	particles	478:486	arg1	SA					510:511	SA	510:511	SA	510:511	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	3	96	with	particles	478:486	arg1	alginate					500:507	sodium alginate	493:507	sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA)	493:564	PCO micro- and nano-sized particles with sodium alginate (SA) as an encapsulating agent (ME-PCO-SA and NE-PCO-SA) were designed by micro and nano spray-drying, respectively, applying a central composite plus star point experimental design.					
35044402	2	97	theme	purified	353:360	arg1	oil					367:369	encapsulated purified chia oil	340:369	encapsulated purified chia oil (PCO)	340:375	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	2	97	theme	purified	353:360	arg1	PCO					372:374	PCO	372:374	PCO	372:374	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	4	98	dep	ME-PCO-SA	781:789	arg1	μm					797:798	0.16 μm vs. 3.5 μm; 98.1%	792:816	μm	797:798	NE-PCO-SA showed a smaller particle size and higher encapsulation efficiency of PCO than ME-PCO-SA (0.16 μm vs. 3.5 μm; 98.1% vs. 92.0%).					
35044402	4	98	dep	ME-PCO-SA	781:789	arg1	%					816:816	0.16 μm vs. 3.5 μm; 98.1%	792:816	%	816:816	NE-PCO-SA showed a smaller particle size and higher encapsulation efficiency of PCO than ME-PCO-SA (0.16 μm vs. 3.5 μm; 98.1% vs. 92.0%).					
35044402	4	98	dep	ME-PCO-SA	781:789	arg1	%					826:826	92.0%	822:826	92.0%	822:826	NE-PCO-SA showed a smaller particle size and higher encapsulation efficiency of PCO than ME-PCO-SA (0.16 μm vs. 3.5 μm; 98.1% vs. 92.0%).					
35044402	7	99	theme	acids	1476:1480	arg1	particles					1388:1396	micro-sized particles	1376:1396	micro-sized particles (ME-PCO-SA, 69.8%)	1376:1415	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	7	99	theme	acids	1476:1480	arg1	bioaccessibility					1434:1449	also higher bioaccessibility	1422:1449	also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area	1422:1570	Nano-sized particles showed a significantly higher PCO release after in vitro digestion (NE-PCO-SA, 78.4%) than micro-sized particles (ME-PCO-SA, 69.8%), and also higher bioaccessibility of individual free fatty acids, such as C18:3 ω-3 (NE-PCO-SA, 23.6%; ME-PCO-SA, 7.9%), due to their greater surface area.					
35044402	2	100	theme	particle	323:330	arg1	size					332:335	the particle size	319:335	the particle size of encapsulated purified chia oil (PCO)	319:375	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	6	101	theme	intestine-site	1232:1245	arg1	polymer					1255:1261	an intestine-site release polymer	1229:1261	an intestine-site release polymer	1229:1261	ME-PCO and NE-PCO showed sustained oil release throughout the three phases of digestion (oral, gastric and intestinal phases), whereas the PCO release from ME-PCO-SA and NE-PCO-SA occurred mainly in the intestinal phase, showing the suitability of sodium alginate as an intestine-site release polymer.					
35044402	2	102	dep	efficiency	398:407	arg1	the					380:382	the	380:382	the	380:382	The aim of this work was to study the influence of the particle size of encapsulated purified chia oil (PCO) on the encapsulation efficiency and PCO release during in vitro digestion.					
35044402	8	103	theme	particle	1668:1675	arg1	systems					1677:1683	both particle systems	1663:1683	both particle systems	1663:1683	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	4	104	theme	particle	719:726	arg1	size					728:731	a smaller particle size	709:731	a smaller particle size	709:731	NE-PCO-SA showed a smaller particle size and higher encapsulation efficiency of PCO than ME-PCO-SA (0.16 μm vs. 3.5 μm; 98.1% vs. 92.0%).					
35044402	9	105	theme	foods	2035:2039	arg1	design					2014:2019	the design	2010:2019	the design of functional foods	2010:2039	Sodium alginate spray-dried micro and nanoparticles showed great potential for vehiculation of omega-3 rich oils in the design of functional foods.					
35044402	8	106	theme	ionic	1845:1849	arg1	gelation					1851:1858	partial ionic gelation	1837:1858	partial ionic gelation of SA	1837:1864	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	5	107	dep	in	918:919	arg1	vitro					921:925	vitro	921:925	vitro	921:925	Emulsions (NE-PCO and ME-PCO) and particles (NE-PCO-SA and ME-PCO-SA) were subjected to in vitro static gastrointestinal digestion.					
35044402	0	108	theme	in	103:104	arg1	digestion					129:137	in vitro gastrointestinal digestion	103:137	in vitro gastrointestinal digestion	103:137	Influence of the particle size of encapsulated chia oil on the oil release and bioaccessibility during in vitro gastrointestinal digestion.					
35044402	8	109	from	systems	1677:1683	arg1	NE-PCO-SA					1732:1740	NE-PCO-SA	1732:1740	NE-PCO-SA	1732:1740	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	8	109	from	systems	1677:1683	arg1	similar					1723:1729	similar	1723:1729	similar	1723:1729	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	8	109	from	systems	1677:1683	arg1	release					1650:1656	the PCO release	1642:1656	the PCO release from both particle systems after the digestion of the matrix	1642:1717	However, when ME-PCO-SA and NE-PCO-SA were incorporated into yogurt, the PCO release from both particle systems after the digestion of the matrix was similar (NE-PCO-SA, 58.8%; ME-PCO-SA-Y, 61.8%), possibly because the calcium ions contained in the yogurt induced partial ionic gelation of SA, impairing the PCO release.					
35044402	9	110	theme	Sodium	1894:1899	arg1	micro					1922:1926	Sodium alginate spray-dried micro	1894:1926	Sodium alginate spray-dried micro	1894:1926	Sodium alginate spray-dried micro and nanoparticles showed great potential for vehiculation of omega-3 rich oils in the design of functional foods.					
35698210	12	0	theme	family	2013:2018	arg1	Members					1982:1988	Members	1982:1988	Members of the Lachnospiraceae family	1982:2018	Members of the Lachnospiraceae family seem to play a central role in this protection.					
35698210	14	1	dep	Abstract	2266:2273	arg1	Video					2260:2264	Video	2260:2264	Video	2260:2264	Video Abstract.					
35698210	3	2	theme	Dectin-1	466:473	arg1	deficiency					538:547	Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency	466:547	Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency	466:547	Here, we explored the role of Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency in intestinal inflammation.					
35698210	8	3	theme	fungal	1311:1316	arg1	microbiota					1318:1327	the fungal microbiota	1307:1327	the fungal microbiota	1307:1327	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents did not affect the protection against gut inflammation, suggesting that the fungal microbiota had no role in the protective phenotype.					
35698210	9	4	theme	Amplicon-based	1370:1383	arg1	analysis					1396:1403	Amplicon-based microbiota analysis	1370:1403	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice	1370:1464	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	8	5	theme	mice	1173:1176	arg1	Treatment					1152:1160	Treatment	1152:1160	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents	1152:1233	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents did not affect the protection against gut inflammation, suggesting that the fungal microbiota had no role in the protective phenotype.					
35698210	11	6	from	effects	1962:1968	arg1	colitis					1973:1979	colitis	1973:1979	colitis	1973:1979	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	8	7	theme	opportunistic	1183:1195	arg1	pathogens					1204:1212	opportunistic fungal pathogens	1183:1212	opportunistic fungal pathogens	1183:1212	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents did not affect the protection against gut inflammation, suggesting that the fungal microbiota had no role in the protective phenotype.					
35698210	9	8	theme	strong	1520:1525	arg1	modification					1527:1538	strong modification	1520:1538	strong modification	1520:1538	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	1	9	from	colitis	249:255	arg1	mice					260:263	mice	260:263	mice	260:263	BACKGROUND Innate immunity genes have been reported to affect susceptibility to inflammatory bowel diseases (IBDs) and colitis in mice.					
35698210	3	10	theme	cell	517:520	arg1	molecules					527:535	fungal cell wall molecules	510:535	fungal cell wall molecules	510:535	Here, we explored the role of Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency in intestinal inflammation.					
35698210	4	11	dep	mice	757:760	arg1	Dectin-1KO					721:730	double Dectin-1KO	714:730	double Dectin-1KO	714:730	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	11	dep	mice	757:760	arg1	mice					757:760	wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice	663:760	mice	757:760	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	11	dep	mice	757:760	arg1	KO					693:694	Dectin-1 knockout (KO)	674:695	Dectin-1 knockout (KO)	674:695	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	11	dep	mice	757:760	arg1	Dectin-2KO					698:707	Dectin-2KO	698:707	Dectin-2KO	698:707	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	11	dep	mice	757:760	arg1	D-1/2KO					748:754	D-1/2KO	748:754	D-1/2KO	748:754	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	12	theme	wild-type	663:671	arg1	Dectin-2KO					736:745	Dectin-2KO	736:745	Dectin-2KO	736:745	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	12	theme	wild-type	663:671	arg1	Dectin-1KO					721:730	double Dectin-1KO	714:730	double Dectin-1KO	714:730	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	12	theme	wild-type	663:671	arg1	mice					757:760	wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice	663:760	mice	757:760	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	12	theme	wild-type	663:671	arg1	KO					693:694	Dectin-1 knockout (KO)	674:695	Dectin-1 knockout (KO)	674:695	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	12	theme	wild-type	663:671	arg1	Dectin-2KO					698:707	Dectin-2KO	698:707	Dectin-2KO	698:707	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	8	13	theme	antifungal	1217:1226	arg1	agents					1228:1233	antifungal agents	1217:1233	antifungal agents	1217:1233	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents did not affect the protection against gut inflammation, suggesting that the fungal microbiota had no role in the protective phenotype.					
35698210	11	14	theme	Dectin-1	1791:1798	arg1	receptors					1813:1821	both the Dectin-1 and Dectin-2 receptors	1782:1821	receptors	1813:1821	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	7	15	theme	gut	1089:1091	arg1	microbiota					1093:1102	the gut microbiota	1085:1102	the gut microbiota	1085:1102	The protection was largely mediated by the gut microbiota, as demonstrated by fecal transfer experiments.					
35698210	1	16	from	susceptibility	192:205	arg1	mice					260:263	mice	260:263	mice	260:263	BACKGROUND Innate immunity genes have been reported to affect susceptibility to inflammatory bowel diseases (IBDs) and colitis in mice.					
35698210	9	17	theme	fungal	1432:1437	arg1	microbiota					1439:1448	the fecal bacterial and fungal microbiota	1408:1448	the fecal bacterial and fungal microbiota of D-1/2KO mice	1408:1464	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	3	18	theme	deficiency	538:547	arg1	role					458:461	the role	454:461	the role of Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency in intestinal inflammation	454:574	Here, we explored the role of Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency in intestinal inflammation.					
35698210	13	19	theme	intestinal	2221:2230	arg1	physiopathology					2232:2246	intestinal physiopathology	2221:2246	intestinal physiopathology	2221:2246	These findings provide new insights into the role of the Dectin receptors, which have been described to date as affecting only the fungal population, in intestinal physiopathology and in IBD.					
35698210	9	20	from	modification	1527:1538	arg1	absence					1480:1486	the absence	1476:1486	the absence of changes in the mycobiota but strong modification of the bacterial microbiota	1476:1566	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	4	21	theme	knockout	683:690	arg1	mice					757:760	wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice	663:760	mice	757:760	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	21	theme	knockout	683:690	arg1	KO					693:694	Dectin-1 knockout (KO)	674:695	Dectin-1 knockout (KO)	674:695	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	9	22	theme	mice	1461:1464	arg1	microbiota					1439:1448	the fecal bacterial and fungal microbiota	1408:1448	the fecal bacterial and fungal microbiota of D-1/2KO mice	1408:1464	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	8	23	with	Treatment	1152:1160	arg1	pathogens					1204:1212	opportunistic fungal pathogens	1183:1212	opportunistic fungal pathogens	1183:1212	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents did not affect the protection against gut inflammation, suggesting that the fungal microbiota had no role in the protective phenotype.					
35698210	8	23	with	Treatment	1152:1160	arg1	agents					1228:1233	antifungal agents	1217:1233	antifungal agents	1217:1233	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents did not affect the protection against gut inflammation, suggesting that the fungal microbiota had no role in the protective phenotype.					
35698210	0	24	theme	bacterial	51:59	arg1	microbiota					80:89	the bacterial but not fungal gut microbiota	47:89	the bacterial but not fungal gut microbiota	47:89	Deletion of both Dectin-1 and Dectin-2 affects the bacterial but not fungal gut microbiota and susceptibility to colitis in mice.					
35698210	5	25	theme	microbiota	818:827	arg1	compositions					829:840	bacterial and fungal microbiota compositions	797:840	bacterial and fungal microbiota compositions	797:840	Inflammation severity, as well as bacterial and fungal microbiota compositions, was monitored.					
35698210	8	26	theme	gut	1273:1275	arg1	inflammation					1277:1288	gut inflammation	1273:1288	gut inflammation	1273:1288	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents did not affect the protection against gut inflammation, suggesting that the fungal microbiota had no role in the protective phenotype.					
35698210	1	27	theme	immunity	148:155	arg1	genes					157:161	BACKGROUND Innate immunity genes	130:161	BACKGROUND Innate immunity genes	130:161	BACKGROUND Innate immunity genes have been reported to affect susceptibility to inflammatory bowel diseases (IBDs) and colitis in mice.					
35698210	9	28	from	changes	1491:1497	arg1	mycobiota					1506:1514	mycobiota	1506:1514	mycobiota	1506:1514	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	9	28	from	changes	1491:1497	arg1	modification					1527:1538	strong modification	1520:1538	strong modification	1520:1538	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	6	29	theme	strong	920:925	arg1	effect					927:932	a strong effect	918:932	a strong effect	918:932	RESULTS While deletion of Dectin-1 or Dectin-2 did not have a strong effect on DSS-induced colitis, double deletion of Dectin-1 and Dectin-2 significantly protected the mice from colitis.					
35698210	11	30	from	shift	1842:1846	arg1	environment					1869:1879	the microbial gut environment	1851:1879	the microbial gut environment	1851:1879	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	0	31	theme	gut	76:78	arg1	microbiota					80:89	the bacterial but not fungal gut microbiota	47:89	the bacterial but not fungal gut microbiota	47:89	Deletion of both Dectin-1 and Dectin-2 affects the bacterial but not fungal gut microbiota and susceptibility to colitis in mice.					
35698210	4	32	theme	double	714:719	arg1	Dectin-1KO					721:730	double Dectin-1KO	714:730	double Dectin-1KO	714:730	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	32	theme	double	714:719	arg1	mice					757:760	wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice	663:760	mice	757:760	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	32	theme	double	714:719	arg1	D-1/2KO					748:754	D-1/2KO	748:754	D-1/2KO	748:754	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	2	33	theme	microbiota	354:363	arg1	modulation					365:374	gut microbiota modulation	350:374	gut microbiota modulation	350:374	Dectin-1, a receptor for fungal cell wall β-glucans, has been clearly implicated in gut microbiota modulation and modification of the susceptibility to gut inflammation.					
35698210	2	34	theme	gut	418:420	arg1	inflammation					422:433	gut inflammation	418:433	gut inflammation	418:433	Dectin-1, a receptor for fungal cell wall β-glucans, has been clearly implicated in gut microbiota modulation and modification of the susceptibility to gut inflammation.					
35698210	6	35	contain	have	913:916	arg2	effect					927:932	a strong effect	918:932	a strong effect	918:932	RESULTS While deletion of Dectin-1 or Dectin-2 did not have a strong effect on DSS-induced colitis, double deletion of Dectin-1 and Dectin-2 significantly protected the mice from colitis.					
35698210	6	35	contain	have	913:916	arg1	deletion					872:879	deletion	872:879	deletion of Dectin-1 or Dectin-2	872:903	RESULTS While deletion of Dectin-1 or Dectin-2 did not have a strong effect on DSS-induced colitis, double deletion of Dectin-1 and Dectin-2 significantly protected the mice from colitis.					
35698210	11	36	theme	microbial	1855:1863	arg1	environment					1869:1879	the microbial gut environment	1851:1879	the microbial gut environment	1851:1879	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	3	37	dep	Dectin-1	466:473	arg1	receptor					497:504	another receptor	489:504	another receptor for fungal cell wall molecules	489:535	Here, we explored the role of Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency in intestinal inflammation.					
35698210	2	38	theme	susceptibility	400:413	arg1	modification					380:391	modification	380:391	modification	380:391	Dectin-1, a receptor for fungal cell wall β-glucans, has been clearly implicated in gut microbiota modulation and modification of the susceptibility to gut inflammation.					
35698210	2	38	theme	susceptibility	400:413	arg1	modulation					365:374	gut microbiota modulation	350:374	gut microbiota modulation	350:374	Dectin-1, a receptor for fungal cell wall β-glucans, has been clearly implicated in gut microbiota modulation and modification of the susceptibility to gut inflammation.					
35698210	9	39	theme	bacterial	1547:1555	arg1	microbiota					1557:1566	the bacterial microbiota	1543:1566	the bacterial microbiota	1543:1566	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	6	40	theme	double	958:963	arg1	deletion					965:972	double deletion	958:972	double deletion of Dectin-1 and Dectin-2	958:997	RESULTS While deletion of Dectin-1 or Dectin-2 did not have a strong effect on DSS-induced colitis, double deletion of Dectin-1 and Dectin-2 significantly protected the mice from colitis.					
35698210	10	41	from	family	1618:1623	arg1	bacteria					1584:1591	bacteria	1584:1591	bacteria from the Lachnospiraceae family	1584:1623	We showed that bacteria from the Lachnospiraceae family were at least partly involved in this protection and that treatment with Blautia hansenii was enough to recapitulate the protection.					
35698210	11	42	dep	shift	1842:1846	arg1	driving					1943:1949	driving	1943:1949	driving protective effects in colitis	1943:1979	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	11	42	dep	shift	1842:1846	arg1	affecting					1882:1890	affecting	1882:1890	affecting, surprisingly, mainly the bacterial population	1882:1937	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	11	43	theme	Dectin-2	1804:1811	arg1	receptors					1813:1821	both the Dectin-1 and Dectin-2 receptors	1782:1821	receptors	1813:1821	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	1	44	theme	inflammatory	210:221	arg1	diseases					229:236	inflammatory bowel diseases	210:236	inflammatory bowel diseases (IBDs)	210:243	BACKGROUND Innate immunity genes have been reported to affect susceptibility to inflammatory bowel diseases (IBDs) and colitis in mice.					
35698210	1	44	theme	inflammatory	210:221	arg1	IBDs					239:242	IBDs	239:242	IBDs	239:242	BACKGROUND Innate immunity genes have been reported to affect susceptibility to inflammatory bowel diseases (IBDs) and colitis in mice.					
35698210	1	45	theme	BACKGROUND	130:139	arg1	genes					157:161	BACKGROUND Innate immunity genes	130:161	BACKGROUND Innate immunity genes	130:161	BACKGROUND Innate immunity genes have been reported to affect susceptibility to inflammatory bowel diseases (IBDs) and colitis in mice.					
35698210	13	46	theme	fungal	2199:2204	arg1	population					2206:2215	only the fungal population	2190:2215	only the fungal population	2190:2215	These findings provide new insights into the role of the Dectin receptors, which have been described to date as affecting only the fungal population, in intestinal physiopathology and in IBD.					
35698210	4	47	theme	DESIGN	577:582	arg1	Susceptibility					584:597	DESIGN Susceptibility	577:597	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis	577:645	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	11	48	theme	global	1835:1840	arg1	shift					1842:1846	a global shift	1833:1846	a global shift	1833:1846	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	12	49	theme	Lachnospiraceae	1997:2011	arg1	family					2013:2018	the Lachnospiraceae family	1993:2018	the Lachnospiraceae family	1993:2018	Members of the Lachnospiraceae family seem to play a central role in this protection.					
35698210	9	50	from	absence	1480:1486	arg1	mycobiota					1506:1514	mycobiota	1506:1514	mycobiota	1506:1514	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	9	50	from	absence	1480:1486	arg1	modification					1527:1538	strong modification	1520:1538	strong modification	1520:1538	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	4	51	theme	-induced	630:637	arg1	colitis					639:645	dextran sodium sulfate (DSS)-induced colitis	602:645	dextran sodium sulfate (DSS)-induced colitis	602:645	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	9	52	theme	microbiota	1385:1394	arg1	analysis					1396:1403	Amplicon-based microbiota analysis	1370:1403	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice	1370:1464	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	8	53	theme	D-1/2KO	1165:1171	arg1	mice					1173:1176	D-1/2KO mice	1165:1176	D-1/2KO mice	1165:1176	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents did not affect the protection against gut inflammation, suggesting that the fungal microbiota had no role in the protective phenotype.					
35698210	3	54	theme	wall	522:525	arg1	molecules					527:535	fungal cell wall molecules	510:535	fungal cell wall molecules	510:535	Here, we explored the role of Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency in intestinal inflammation.					
35698210	5	55	theme	bacterial	797:805	arg1	compositions					829:840	bacterial and fungal microbiota compositions	797:840	bacterial and fungal microbiota compositions	797:840	Inflammation severity, as well as bacterial and fungal microbiota compositions, was monitored.					
35698210	3	56	from	role	458:461	arg1	inflammation					563:574	intestinal inflammation	552:574	intestinal inflammation	552:574	Here, we explored the role of Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency in intestinal inflammation.					
35698210	3	57	theme	fungal	510:515	arg1	molecules					527:535	fungal cell wall molecules	510:535	fungal cell wall molecules	510:535	Here, we explored the role of Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency in intestinal inflammation.					
35698210	9	58	theme	fecal	1412:1416	arg1	microbiota					1439:1448	the fecal bacterial and fungal microbiota	1408:1448	the fecal bacterial and fungal microbiota of D-1/2KO mice	1408:1464	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	6	59	theme	Dectin-2	896:903	arg1	deletion					872:879	deletion	872:879	deletion of Dectin-1 or Dectin-2	872:903	RESULTS While deletion of Dectin-1 or Dectin-2 did not have a strong effect on DSS-induced colitis, double deletion of Dectin-1 and Dectin-2 significantly protected the mice from colitis.					
35698210	8	60	theme	fungal	1197:1202	arg1	pathogens					1204:1212	opportunistic fungal pathogens	1183:1212	opportunistic fungal pathogens	1183:1212	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents did not affect the protection against gut inflammation, suggesting that the fungal microbiota had no role in the protective phenotype.					
35698210	11	61	theme	CONCLUSIONS	1758:1768	arg1	Deletion					1770:1777	CONCLUSIONS Deletion	1758:1777	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors	1758:1821	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	13	62	theme	new	2091:2093	arg1	insights					2095:2102	new insights	2091:2102	new insights into the role of the Dectin receptors, which have been described to date as affecting only the fungal population, in intestinal physiopathology and in IBD	2091:2257	These findings provide new insights into the role of the Dectin receptors, which have been described to date as affecting only the fungal population, in intestinal physiopathology and in IBD.					
35698210	11	63	theme	bacterial	1918:1926	arg1	population					1928:1937	mainly the bacterial population	1907:1937	mainly the bacterial population	1907:1937	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	6	64	theme	Dectin-1	884:891	arg1	deletion					872:879	deletion	872:879	deletion of Dectin-1 or Dectin-2	872:903	RESULTS While deletion of Dectin-1 or Dectin-2 did not have a strong effect on DSS-induced colitis, double deletion of Dectin-1 and Dectin-2 significantly protected the mice from colitis.					
35698210	9	65	theme	microbiota	1439:1448	arg1	analysis					1396:1403	Amplicon-based microbiota analysis	1370:1403	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice	1370:1464	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	0	66	from	susceptibility	95:108	arg1	mice					124:127	mice	124:127	mice	124:127	Deletion of both Dectin-1 and Dectin-2 affects the bacterial but not fungal gut microbiota and susceptibility to colitis in mice.					
35698210	12	67	theme	central	2035:2041	arg1	role					2043:2046	a central role	2033:2046	a central role	2033:2046	Members of the Lachnospiraceae family seem to play a central role in this protection.					
35698210	9	68	dep	mycobiota	1506:1514	arg1	the					1502:1504	the	1502:1504	the	1502:1504	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	11	69	theme	protective	1951:1960	arg1	effects					1962:1968	protective effects	1951:1968	protective effects in colitis	1951:1979	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	9	70	theme	D-1/2KO	1453:1459	arg1	mice					1461:1464	D-1/2KO mice	1453:1464	D-1/2KO mice	1453:1464	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	0	71	from	microbiota	80:89	arg1	mice					124:127	mice	124:127	mice	124:127	Deletion of both Dectin-1 and Dectin-2 affects the bacterial but not fungal gut microbiota and susceptibility to colitis in mice.					
35698210	4	72	theme	Dectin-1	674:681	arg1	mice					757:760	wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice	663:760	mice	757:760	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	4	72	theme	Dectin-1	674:681	arg1	KO					693:694	Dectin-1 knockout (KO)	674:695	Dectin-1 knockout (KO)	674:695	DESIGN Susceptibility to dextran sodium sulfate (DSS)-induced colitis was assessed in wild-type, Dectin-1 knockout (KO), Dectin-2KO, and double Dectin-1KO and Dectin-2KO (D-1/2KO) mice.					
35698210	2	73	theme	cell	298:301	arg1	β-glucans					308:316	fungal cell wall β-glucans	291:316	fungal cell wall β-glucans	291:316	Dectin-1, a receptor for fungal cell wall β-glucans, has been clearly implicated in gut microbiota modulation and modification of the susceptibility to gut inflammation.					
35698210	5	74	theme	Inflammation	763:774	arg1	severity					776:783	Inflammation severity	763:783	Inflammation severity	763:783	Inflammation severity, as well as bacterial and fungal microbiota compositions, was monitored.					
35698210	5	75	theme	fungal	811:816	arg1	compositions					829:840	bacterial and fungal microbiota compositions	797:840	bacterial and fungal microbiota compositions	797:840	Inflammation severity, as well as bacterial and fungal microbiota compositions, was monitored.					
35698210	6	76	theme	DSS-induced	937:947	arg1	colitis					949:955	DSS-induced colitis	937:955	DSS-induced colitis	937:955	RESULTS While deletion of Dectin-1 or Dectin-2 did not have a strong effect on DSS-induced colitis, double deletion of Dectin-1 and Dectin-2 significantly protected the mice from colitis.					
35698210	8	77	contain	had	1329:1331	arg1	microbiota					1318:1327	the fungal microbiota	1307:1327	the fungal microbiota	1307:1327	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents did not affect the protection against gut inflammation, suggesting that the fungal microbiota had no role in the protective phenotype.					
35698210	8	77	contain	had	1329:1331	arg2	role					1336:1339	no role	1333:1339	no role	1333:1339	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents did not affect the protection against gut inflammation, suggesting that the fungal microbiota had no role in the protective phenotype.					
35698210	13	78	theme	Dectin	2125:2130	arg1	receptors					2132:2140	the Dectin receptors	2121:2140	the Dectin receptors	2121:2140	These findings provide new insights into the role of the Dectin receptors, which have been described to date as affecting only the fungal population, in intestinal physiopathology and in IBD.					
35698210	7	79	theme	transfer	1130:1137	arg1	experiments					1139:1149	fecal transfer experiments	1124:1149	fecal transfer experiments	1124:1149	The protection was largely mediated by the gut microbiota, as demonstrated by fecal transfer experiments.					
35698210	0	80	theme	fungal	69:74	arg1	microbiota					80:89	the bacterial but not fungal gut microbiota	47:89	the bacterial but not fungal gut microbiota	47:89	Deletion of both Dectin-1 and Dectin-2 affects the bacterial but not fungal gut microbiota and susceptibility to colitis in mice.					
35698210	1	81	theme	Innate	141:146	arg1	genes					157:161	BACKGROUND Innate immunity genes	130:161	BACKGROUND Innate immunity genes	130:161	BACKGROUND Innate immunity genes have been reported to affect susceptibility to inflammatory bowel diseases (IBDs) and colitis in mice.					
35698210	9	82	from	mycobiota	1506:1514	arg1	absence					1480:1486	the absence	1476:1486	the absence of changes in the mycobiota but strong modification of the bacterial microbiota	1476:1566	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	7	83	theme	fecal	1124:1128	arg1	experiments					1139:1149	fecal transfer experiments	1124:1149	fecal transfer experiments	1124:1149	The protection was largely mediated by the gut microbiota, as demonstrated by fecal transfer experiments.					
35698210	9	84	theme	changes	1491:1497	arg1	absence					1480:1486	the absence	1476:1486	the absence of changes in the mycobiota but strong modification of the bacterial microbiota	1476:1566	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	11	85	theme	receptors	1813:1821	arg1	Deletion					1770:1777	CONCLUSIONS Deletion	1758:1777	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors	1758:1821	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	9	86	theme	bacterial	1418:1426	arg1	microbiota					1439:1448	the fecal bacterial and fungal microbiota	1408:1448	the fecal bacterial and fungal microbiota of D-1/2KO mice	1408:1464	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	13	87	theme	receptors	2132:2140	arg1	role					2113:2116	the role	2109:2116	the role of the Dectin receptors, which have been described to date as affecting only the fungal population, in intestinal physiopathology and in IBD	2109:2257	These findings provide new insights into the role of the Dectin receptors, which have been described to date as affecting only the fungal population, in intestinal physiopathology and in IBD.					
35698210	2	88	theme	gut	350:352	arg1	modulation					365:374	gut microbiota modulation	350:374	gut microbiota modulation	350:374	Dectin-1, a receptor for fungal cell wall β-glucans, has been clearly implicated in gut microbiota modulation and modification of the susceptibility to gut inflammation.					
35698210	6	89	theme	Dectin-2	990:997	arg1	deletion					965:972	double deletion	958:972	double deletion of Dectin-1 and Dectin-2	958:997	RESULTS While deletion of Dectin-1 or Dectin-2 did not have a strong effect on DSS-induced colitis, double deletion of Dectin-1 and Dectin-2 significantly protected the mice from colitis.					
35698210	11	90	theme	gut	1865:1867	arg1	environment					1869:1879	the microbial gut environment	1851:1879	the microbial gut environment	1851:1879	CONCLUSIONS Deletion of both the Dectin-1 and Dectin-2 receptors triggered a global shift in the microbial gut environment, affecting, surprisingly, mainly the bacterial population and driving protective effects in colitis.					
35698210	0	91	theme	Dectin-1	17:24	arg1	Deletion					0:7	Deletion	0:7	Deletion of both Dectin-1 and Dectin-2	0:37	Deletion of both Dectin-1 and Dectin-2 affects the bacterial but not fungal gut microbiota and susceptibility to colitis in mice.					
35698210	6	92	theme	Dectin-1	977:984	arg1	deletion					965:972	double deletion	958:972	double deletion of Dectin-1 and Dectin-2	958:997	RESULTS While deletion of Dectin-1 or Dectin-2 did not have a strong effect on DSS-induced colitis, double deletion of Dectin-1 and Dectin-2 significantly protected the mice from colitis.					
35698210	0	93	theme	Dectin-2	30:37	arg1	Deletion					0:7	Deletion	0:7	Deletion of both Dectin-1 and Dectin-2	0:37	Deletion of both Dectin-1 and Dectin-2 affects the bacterial but not fungal gut microbiota and susceptibility to colitis in mice.					
35698210	13	94	from	role	2113:2116	arg1	physiopathology					2232:2246	intestinal physiopathology	2221:2246	intestinal physiopathology	2221:2246	These findings provide new insights into the role of the Dectin receptors, which have been described to date as affecting only the fungal population, in intestinal physiopathology and in IBD.					
35698210	13	94	from	role	2113:2116	arg1	IBD					2255:2257	IBD	2255:2257	IBD	2255:2257	These findings provide new insights into the role of the Dectin receptors, which have been described to date as affecting only the fungal population, in intestinal physiopathology and in IBD.					
35698210	9	95	theme	microbiota	1557:1566	arg1	mycobiota					1506:1514	mycobiota	1506:1514	mycobiota	1506:1514	Amplicon-based microbiota analysis of the fecal bacterial and fungal microbiota of D-1/2KO mice confirmed the absence of changes in the mycobiota but strong modification of the bacterial microbiota.					
35698210	2	96	theme	wall	303:306	arg1	β-glucans					308:316	fungal cell wall β-glucans	291:316	fungal cell wall β-glucans	291:316	Dectin-1, a receptor for fungal cell wall β-glucans, has been clearly implicated in gut microbiota modulation and modification of the susceptibility to gut inflammation.					
35698210	3	97	theme	intestinal	552:561	arg1	inflammation					563:574	intestinal inflammation	552:574	intestinal inflammation	552:574	Here, we explored the role of Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency in intestinal inflammation.					
35698210	8	98	theme	protective	1348:1357	arg1	phenotype					1359:1367	the protective phenotype	1344:1367	the protective phenotype	1344:1367	Treatment of D-1/2KO mice with opportunistic fungal pathogens or antifungal agents did not affect the protection against gut inflammation, suggesting that the fungal microbiota had no role in the protective phenotype.					
35698210	2	99	theme	fungal	291:296	arg1	β-glucans					308:316	fungal cell wall β-glucans	291:316	fungal cell wall β-glucans	291:316	Dectin-1, a receptor for fungal cell wall β-glucans, has been clearly implicated in gut microbiota modulation and modification of the susceptibility to gut inflammation.					
35698210	1	100	theme	bowel	223:227	arg1	diseases					229:236	inflammatory bowel diseases	210:236	inflammatory bowel diseases (IBDs)	210:243	BACKGROUND Innate immunity genes have been reported to affect susceptibility to inflammatory bowel diseases (IBDs) and colitis in mice.					
35698210	1	100	theme	bowel	223:227	arg1	IBDs					239:242	IBDs	239:242	IBDs	239:242	BACKGROUND Innate immunity genes have been reported to affect susceptibility to inflammatory bowel diseases (IBDs) and colitis in mice.					
35698210	10	101	with	treatment	1683:1691	arg1	Blautia					1698:1704	Blautia	1698:1704	Blautia	1698:1704	We showed that bacteria from the Lachnospiraceae family were at least partly involved in this protection and that treatment with Blautia hansenii was enough to recapitulate the protection.					
35698210	10	102	theme	Lachnospiraceae	1602:1616	arg1	family					1618:1623	the Lachnospiraceae family	1598:1623	the Lachnospiraceae family	1598:1623	We showed that bacteria from the Lachnospiraceae family were at least partly involved in this protection and that treatment with Blautia hansenii was enough to recapitulate the protection.					
35698210	3	103	theme	Dectin-2	479:486	arg1	deficiency					538:547	Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency	466:547	Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency	466:547	Here, we explored the role of Dectin-1 and Dectin-2 (another receptor for fungal cell wall molecules) deficiency in intestinal inflammation.					
37363916	6	0	theme	proteomics	1010:1019	arg1	analysis					1021:1028	proteomics analysis	1010:1028	proteomics analysis	1010:1028	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	1	1	theme	regulatory	254:263	arg1	cells					274:278	IL-10-producing regulatory B (Breg) cells	238:278	IL-10-producing regulatory B (Breg) cells	238:278	During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role.					
37363916	0	2	link	egg-derived	20:30	arg1	thioredoxin					32:42	egg-derived thioredoxin	20:42	egg-derived thioredoxin	20:42	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	0	2	link	egg-derived	20:30	arg1	mansoni					12:18	Schistosoma mansoni egg-derived thioredoxin and Sm14	0:51	Schistosoma mansoni egg-derived thioredoxin and Sm14	0:51	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	6	3	theme	putative	1098:1105	arg1	Sm14					1200:1203	the fatty acid binding protein Sm14	1169:1203	the fatty acid binding protein Sm14	1169:1203	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	6	3	theme	putative	1098:1105	arg1	properties					1124:1133	putative immunomodulatory properties	1098:1133	putative immunomodulatory properties	1098:1133	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	6	3	theme	putative	1098:1105	arg1	thioredoxin					1144:1154	thioredoxin	1144:1154	thioredoxin (SmTrx1)	1144:1163	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	6	4	theme	binding	1184:1190	arg1	protein					1192:1198	the fatty acid binding protein	1169:1198	the fatty acid binding protein Sm14	1169:1203	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	6	5	theme	proteins	1084:1091	arg1	number					1074:1079	a number	1072:1079	a number of proteins	1072:1091	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	6	6	theme	fatty	1173:1177	arg1	protein					1192:1198	the fatty acid binding protein	1169:1198	the fatty acid binding protein Sm14	1169:1203	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	3	7	dep	found	550:554	arg1	18					619:620	18	619:620	18	619:620	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	4	8	theme	multi-fucosylated	757:773	arg1	proteins					775:782	multi-fucosylated proteins	757:782	multi-fucosylated proteins	757:782	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	5	9	theme	SEA	791:793	arg1	glycoproteins					795:807	SEA glycoproteins	791:807	SEA glycoproteins purified by affinity chromatography and synthetic glycans coupled to gold nanoparticles	791:895	Using SEA glycoproteins purified by affinity chromatography and synthetic glycans coupled to gold nanoparticles, we investigated the role of these glycan structures in inducing IL-10 production by B cells.					
37363916	3	10	theme	molecular	651:659	arg1	MW					669:670	MW	669:670	MW	669:670	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	3	10	theme	molecular	651:659	arg1	weight					661:666	molecular weight	651:666	the high, medium and low molecular weight (MW) range	626:677	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	3	11	theme	size	516:519	arg1	chromatography					531:544	size exclusion chromatography	516:544	size exclusion chromatography	516:544	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	1	12	theme	immune	214:219	arg1	system					221:226	the host immune system	205:226	the host immune system	205:226	During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role.					
37363916	7	13	with	stimulations	1239:1250	arg1	Sm14					1303:1306	Sm14	1303:1306	Sm14	1303:1306	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	7	13	with	stimulations	1239:1250	arg1	SmTrx1					1292:1297	recombinant SmTrx1	1280:1297	recombinant SmTrx1	1280:1297	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	1	14	theme	chronic	121:127	arg1	infections					141:150	chronic schistosome infections	121:150	chronic schistosome infections	121:150	During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role.					
37363916	7	15	theme	recombinant	1280:1290	arg1	SmTrx1					1292:1297	recombinant SmTrx1	1280:1297	recombinant SmTrx1	1280:1297	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	5	16	theme	B	982:982	arg1	cells					984:988	B cells	982:988	B cells	982:988	Using SEA glycoproteins purified by affinity chromatography and synthetic glycans coupled to gold nanoparticles, we investigated the role of these glycan structures in inducing IL-10 production by B cells.					
37363916	3	17	theme	IL-10	583:587	arg1	production					589:598	IL-10 production	583:598	IL-10 production	583:598	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	1	18	theme	schistosome	129:139	arg1	infections					141:150	chronic schistosome infections	121:150	chronic schistosome infections	121:150	During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role.					
37363916	4	19	gly	glycosylated	723:734	arg1	molecules					736:744	heavily glycosylated molecules	715:744	heavily glycosylated molecules	715:744	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	4	19	gly	glycosylated	723:734	arg1	proteins					775:782	multi-fucosylated proteins	757:782	multi-fucosylated proteins	757:782	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	0	20	theme	IL-10	78:82	arg1	cells					107:111	IL-10 producing regulatory B cells	78:111	IL-10 producing regulatory B cells	78:111	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	1	21	theme	regulatory	163:172	arg1	network					174:180	a complex regulatory network	153:180	a complex regulatory network	153:180	During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role.					
37363916	8	22	theme	unique	1429:1434	arg1	molecules					1456:1464	unique Breg cells-inducing molecules	1429:1464	unique Breg cells-inducing molecules	1429:1464	Identification of unique Breg cells-inducing molecules may pave the way to innovative therapeutic strategies for inflammatory and auto-immune diseases.					
37363916	0	23	theme	regulatory	94:103	arg1	cells					107:111	IL-10 producing regulatory B cells	78:111	IL-10 producing regulatory B cells	78:111	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	8	24	theme	cells-inducing	1441:1454	arg1	molecules					1456:1464	unique Breg cells-inducing molecules	1429:1464	unique Breg cells-inducing molecules	1429:1464	Identification of unique Breg cells-inducing molecules may pave the way to innovative therapeutic strategies for inflammatory and auto-immune diseases.					
37363916	0	25	theme	cells	107:111	arg1	development					63:73	the development	59:73	the development of IL-10 producing regulatory B cells	59:111	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	7	26	theme	hock	1256:1259	arg1	immunizations					1261:1273	hock immunizations	1256:1273	hock immunizations with recombinant SmTrx1 and Sm14	1256:1306	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	0	27	theme	Schistosoma	0:10	arg1	Sm14					48:51	Sm14	48:51	Sm14	48:51	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	0	27	theme	Schistosoma	0:10	arg1	thioredoxin					32:42	egg-derived thioredoxin	20:42	egg-derived thioredoxin	20:42	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	0	27	theme	Schistosoma	0:10	arg1	mansoni					12:18	Schistosoma mansoni egg-derived thioredoxin and Sm14	0:51	Schistosoma mansoni egg-derived thioredoxin and Sm14	0:51	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	1	28	theme	Breg	268:271	arg1	cells					274:278	IL-10-producing regulatory B (Breg) cells	238:278	IL-10-producing regulatory B (Breg) cells	238:278	During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role.					
37363916	2	29	dep	human	406:410	arg1	IL-10					422:426	IL-10	422:426	IL-10	422:426	Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells.					
37363916	0	30	theme	egg-derived	20:30	arg1	thioredoxin					32:42	egg-derived thioredoxin	20:42	egg-derived thioredoxin	20:42	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	0	30	theme	egg-derived	20:30	arg1	mansoni					12:18	Schistosoma mansoni egg-derived thioredoxin and Sm14	0:51	Schistosoma mansoni egg-derived thioredoxin and Sm14	0:51	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	7	31	theme	IL-10	1356:1360	arg1	production					1362:1371	IL-10 production	1356:1371	IL-10 production	1356:1371	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	7	32	theme	B	1232:1232	arg1	stimulations					1239:1250	Subsequent splenic murine B cell stimulations	1206:1250	Subsequent splenic murine B cell stimulations	1206:1250	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	2	33	theme	Breg	438:441	arg1	cells					443:447	Breg cells	438:447	Breg cells	438:447	Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells.					
37363916	2	34	theme	B	382:382	arg1	cells					384:388	B cells	382:388	B cells	382:388	Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells.					
37363916	7	35	theme	splenic	1217:1223	arg1	stimulations					1239:1250	Subsequent splenic murine B cell stimulations	1206:1250	Subsequent splenic murine B cell stimulations	1206:1250	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	8	36	theme	therapeutic	1497:1507	arg1	strategies					1509:1518	innovative therapeutic strategies	1486:1518	innovative therapeutic strategies	1486:1518	Identification of unique Breg cells-inducing molecules may pave the way to innovative therapeutic strategies for inflammatory and auto-immune diseases.					
37363916	4	37	theme	glycosylated	723:734	arg1	molecules					736:744	heavily glycosylated molecules	715:744	heavily glycosylated molecules	715:744	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	4	37	theme	glycosylated	723:734	arg1	proteins					775:782	multi-fucosylated proteins	757:782	multi-fucosylated proteins	757:782	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	2	38	theme	egg	333:335	arg1	SEA					347:349	SEA	347:349	SEA	347:349	Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells.					
37363916	2	38	theme	egg	333:335	arg1	antigens					337:344	Schistosoma mansoni soluble egg antigens	305:344	Schistosoma mansoni soluble egg antigens (SEA)	305:350	Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells.					
37363916	5	39	theme	IL-10	962:966	arg1	production					968:977	IL-10 production	962:977	IL-10 production	962:977	Using SEA glycoproteins purified by affinity chromatography and synthetic glycans coupled to gold nanoparticles, we investigated the role of these glycan structures in inducing IL-10 production by B cells.					
37363916	6	40	theme	MW	1044:1045	arg1	fractions					1047:1055	active low MW fractions	1033:1055	active low MW fractions	1033:1055	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	4	41	theme	MW	689:690	arg1	fractions					692:700	The high MW fractions	680:700	The high MW fractions	680:700	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	4	41	theme	MW	689:690	arg1	rich					707:710	rich	707:710	rich	707:710	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	2	42	theme	mansoni	317:323	arg1	SEA					347:349	SEA	347:349	SEA	347:349	Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells.					
37363916	2	42	theme	mansoni	317:323	arg1	antigens					337:344	Schistosoma mansoni soluble egg antigens	305:344	Schistosoma mansoni soluble egg antigens (SEA)	305:350	Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells.					
37363916	6	43	theme	active	1033:1038	arg1	fractions					1047:1055	active low MW fractions	1033:1055	active low MW fractions	1033:1055	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	5	44	theme	glycan	932:937	arg1	structures					939:948	these glycan structures	926:948	these glycan structures	926:948	Using SEA glycoproteins purified by affinity chromatography and synthetic glycans coupled to gold nanoparticles, we investigated the role of these glycan structures in inducing IL-10 production by B cells.					
37363916	3	45	theme	high	630:633	arg1	range					673:677	the high, medium and low molecular weight (MW) range	626:677	the high, medium and low molecular weight (MW) range	626:677	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	3	46	theme	Breg-inducing	462:474	arg1	proteins					476:483	Breg-inducing proteins	462:483	Breg-inducing proteins in SEA	462:490	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	1	47	theme	IL-10-producing	238:252	arg1	cells					274:278	IL-10-producing regulatory B (Breg) cells	238:278	IL-10-producing regulatory B (Breg) cells	238:278	During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role.					
37363916	6	48	theme	protein	1192:1198	arg1	Sm14					1200:1203	the fatty acid binding protein Sm14	1169:1203	the fatty acid binding protein Sm14	1169:1203	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	6	48	theme	protein	1192:1198	arg1	properties					1124:1133	putative immunomodulatory properties	1098:1133	putative immunomodulatory properties	1098:1133	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	3	49	from	proteins	476:483	arg1	SEA					488:490	SEA	488:490	SEA	488:490	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	1	50	theme	B	265:265	arg1	cells					274:278	IL-10-producing regulatory B (Breg) cells	238:278	IL-10-producing regulatory B (Breg) cells	238:278	During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role.					
37363916	7	51	theme	B	1376:1376	arg1	cells					1378:1382	B cells	1376:1382	B cells	1376:1382	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	3	52	theme	low	647:649	arg1	range					673:677	the high, medium and low molecular weight (MW) range	626:677	the high, medium and low molecular weight (MW) range	626:677	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	6	53	theme	acid	1179:1182	arg1	protein					1192:1198	the fatty acid binding protein	1169:1198	the fatty acid binding protein Sm14	1169:1203	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	3	54	theme	medium	636:641	arg1	range					673:677	the high, medium and low molecular weight (MW) range	626:677	the high, medium and low molecular weight (MW) range	626:677	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	3	55	theme	exclusion	521:529	arg1	chromatography					531:544	size exclusion chromatography	516:544	size exclusion chromatography	516:544	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	5	56	theme	affinity	821:828	arg1	chromatography					830:843	affinity chromatography	821:843	affinity chromatography	821:843	Using SEA glycoproteins purified by affinity chromatography and synthetic glycans coupled to gold nanoparticles, we investigated the role of these glycan structures in inducing IL-10 production by B cells.					
37363916	3	57	theme	weight	661:666	arg1	range					673:677	the high, medium and low molecular weight (MW) range	626:677	the high, medium and low molecular weight (MW) range	626:677	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	1	58	theme	host	209:212	arg1	system					221:226	the host immune system	205:226	the host immune system	205:226	During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role.					
37363916	5	59	gly	glycoproteins	795:807	arg1	glycoproteins					795:807	SEA glycoproteins	791:807	SEA glycoproteins purified by affinity chromatography and synthetic glycans coupled to gold nanoparticles	791:895	Using SEA glycoproteins purified by affinity chromatography and synthetic glycans coupled to gold nanoparticles, we investigated the role of these glycan structures in inducing IL-10 production by B cells.					
37363916	4	60	from	rich	707:710	arg1	molecules					736:744	heavily glycosylated molecules	715:744	heavily glycosylated molecules	715:744	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	4	60	from	rich	707:710	arg1	proteins					775:782	multi-fucosylated proteins	757:782	multi-fucosylated proteins	757:782	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	5	61	theme	synthetic	849:857	arg1	glycans					859:865	synthetic glycans	849:865	synthetic glycans	849:865	Using SEA glycoproteins purified by affinity chromatography and synthetic glycans coupled to gold nanoparticles, we investigated the role of these glycan structures in inducing IL-10 production by B cells.					
37363916	7	62	with	immunizations	1261:1273	arg1	Sm14					1303:1306	Sm14	1303:1306	Sm14	1303:1306	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	7	62	with	immunizations	1261:1273	arg1	SmTrx1					1292:1297	recombinant SmTrx1	1280:1297	recombinant SmTrx1	1280:1297	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	3	63	theme	able	568:571	arg1	fractions					558:566	6 fractions	556:566	6 fractions able to induce IL-10 production by B cells	556:609	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	6	64	theme	immunomodulatory	1107:1122	arg1	Sm14					1200:1203	the fatty acid binding protein Sm14	1169:1203	the fatty acid binding protein Sm14	1169:1203	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	6	64	theme	immunomodulatory	1107:1122	arg1	properties					1124:1133	putative immunomodulatory properties	1098:1133	putative immunomodulatory properties	1098:1133	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	6	64	theme	immunomodulatory	1107:1122	arg1	thioredoxin					1144:1154	thioredoxin	1144:1154	thioredoxin (SmTrx1)	1144:1163	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	0	65	theme	producing	84:92	arg1	cells					107:111	IL-10 producing regulatory B cells	78:111	IL-10 producing regulatory B cells	78:111	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	1	66	theme	complex	155:161	arg1	network					174:180	a complex regulatory network	153:180	a complex regulatory network	153:180	During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role.					
37363916	8	67	theme	Breg	1436:1439	arg1	molecules					1456:1464	unique Breg cells-inducing molecules	1429:1464	unique Breg cells-inducing molecules	1429:1464	Identification of unique Breg cells-inducing molecules may pave the way to innovative therapeutic strategies for inflammatory and auto-immune diseases.					
37363916	0	68	theme	B	105:105	arg1	cells					107:111	IL-10 producing regulatory B cells	78:111	IL-10 producing regulatory B cells	78:111	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	8	69	theme	molecules	1456:1464	arg1	Identification					1411:1424	Identification	1411:1424	Identification of unique Breg cells-inducing molecules	1411:1464	Identification of unique Breg cells-inducing molecules may pave the way to innovative therapeutic strategies for inflammatory and auto-immune diseases.					
37363916	4	70	from	molecules	736:744	arg1	fractions					692:700	The high MW fractions	680:700	The high MW fractions	680:700	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	4	70	from	molecules	736:744	arg1	rich					707:710	rich	707:710	rich	707:710	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	7	71	theme	cell	1234:1237	arg1	stimulations					1239:1250	Subsequent splenic murine B cell stimulations	1206:1250	Subsequent splenic murine B cell stimulations	1206:1250	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	5	72	theme	gold	878:881	arg1	nanoparticles					883:895	gold nanoparticles	878:895	gold nanoparticles	878:895	Using SEA glycoproteins purified by affinity chromatography and synthetic glycans coupled to gold nanoparticles, we investigated the role of these glycan structures in inducing IL-10 production by B cells.					
37363916	7	73	theme	murine	1225:1230	arg1	stimulations					1239:1250	Subsequent splenic murine B cell stimulations	1206:1250	Subsequent splenic murine B cell stimulations	1206:1250	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	8	74	theme	innovative	1486:1495	arg1	strategies					1509:1518	innovative therapeutic strategies	1486:1518	innovative therapeutic strategies	1486:1518	Identification of unique Breg cells-inducing molecules may pave the way to innovative therapeutic strategies for inflammatory and auto-immune diseases.					
37363916	3	75	theme	B	603:603	arg1	cells					605:609	B cells	603:609	B cells	603:609	To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.					
37363916	5	76	theme	structures	939:948	arg1	role					918:921	the role	914:921	the role of these glycan structures in inducing IL-10 production by B cells	914:988	Using SEA glycoproteins purified by affinity chromatography and synthetic glycans coupled to gold nanoparticles, we investigated the role of these glycan structures in inducing IL-10 production by B cells.					
37363916	7	77	theme	Subsequent	1206:1215	arg1	stimulations					1239:1250	Subsequent splenic murine B cell stimulations	1206:1250	Subsequent splenic murine B cell stimulations	1206:1250	Subsequent splenic murine B cell stimulations and hock immunizations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production by B cells both in vitro and in vivo.					
37363916	1	78	theme	significant	287:297	arg1	role					299:302	a significant role	285:302	a significant role	285:302	During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role.					
37363916	2	79	theme	soluble	325:331	arg1	SEA					347:349	SEA	347:349	SEA	347:349	Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells.					
37363916	2	79	theme	soluble	325:331	arg1	antigens					337:344	Schistosoma mansoni soluble egg antigens	305:344	Schistosoma mansoni soluble egg antigens (SEA)	305:350	Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells.					
37363916	6	80	theme	low	1040:1042	arg1	fractions					1047:1055	active low MW fractions	1033:1055	active low MW fractions	1033:1055	Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14.					
37363916	8	81	theme	auto-immune	1541:1551	arg1	diseases					1553:1560	auto-immune diseases	1541:1560	auto-immune diseases	1541:1560	Identification of unique Breg cells-inducing molecules may pave the way to innovative therapeutic strategies for inflammatory and auto-immune diseases.					
37363916	4	82	theme	high	684:687	arg1	fractions					692:700	The high MW fractions	680:700	The high MW fractions	680:700	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	4	82	theme	high	684:687	arg1	rich					707:710	rich	707:710	rich	707:710	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	2	83	theme	Schistosoma	305:315	arg1	SEA					347:349	SEA	347:349	SEA	347:349	Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells.					
37363916	2	83	theme	Schistosoma	305:315	arg1	antigens					337:344	Schistosoma mansoni soluble egg antigens	305:344	Schistosoma mansoni soluble egg antigens (SEA)	305:350	Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells.					
37363916	4	84	gly	multi-fucosylated	757:773	arg1	proteins					775:782	multi-fucosylated proteins	757:782	multi-fucosylated proteins	757:782	The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins.					
37363916	0	85	dep	mansoni	12:18	arg1	Sm14					48:51	Sm14	48:51	Sm14	48:51	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	0	85	dep	mansoni	12:18	arg1	thioredoxin					32:42	egg-derived thioredoxin	20:42	egg-derived thioredoxin	20:42	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
37363916	0	85	dep	mansoni	12:18	arg1	mansoni					12:18	Schistosoma mansoni egg-derived thioredoxin and Sm14	0:51	Schistosoma mansoni egg-derived thioredoxin and Sm14	0:51	Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.					
35671132	0	0	theme	hepatic	85:91	arg1	metabolome					93:102	the hepatic metabolome	81:102	the hepatic metabolome in high-fat diet-fed mice	81:128	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	2	1	theme	-α-D-Glcp	484:492	arg1	-α-D-Glcp					484:492	(1 → 4)-α-D-Glcp	477:492	(1 → 4)-α-D-Glcp	477:492	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	1	theme	-α-D-Glcp	484:492	arg1	residues					425:432	the sugar residues	415:432	the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp	415:492	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	1	theme	-α-D-Glcp	484:492	arg1	-α-D-Glcp					462:470	(1 → 2)-α-D-Glcp	455:470	(1 → 2)-α-D-Glcp	455:470	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	1	theme	-α-D-Glcp	484:492	arg1	-β-D-Glcp					444:452	(1 → 2)-β-D-Glcp	437:452	(1 → 2)-β-D-Glcp	437:452	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	1	2	theme	novel	146:150	arg1	PCO					182:184	PCO	182:184	PCO	182:184	In this study, novel Poria cocos oligosaccharides (PCO) were prepared by enzymatic degradation, and their polymerization degree was determined to be 2-6 by LC-MS analysis.					
35671132	1	2	theme	novel	146:150	arg1	oligosaccharides					164:179	novel Poria cocos oligosaccharides	146:179	novel Poria cocos oligosaccharides (PCO)	146:185	In this study, novel Poria cocos oligosaccharides (PCO) were prepared by enzymatic degradation, and their polymerization degree was determined to be 2-6 by LC-MS analysis.					
35671132	2	3	theme	NMR	373:375	arg1	analysis					377:384	NMR analysis	373:384	NMR analysis	373:384	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	0	4	from	characterization	11:26	arg1	metabolome					93:102	the hepatic metabolome	81:102	the hepatic metabolome in high-fat diet-fed mice	81:128	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	1	5	theme	Poria	152:156	arg1	PCO					182:184	PCO	182:184	PCO	182:184	In this study, novel Poria cocos oligosaccharides (PCO) were prepared by enzymatic degradation, and their polymerization degree was determined to be 2-6 by LC-MS analysis.					
35671132	1	5	theme	Poria	152:156	arg1	oligosaccharides					164:179	novel Poria cocos oligosaccharides	146:179	novel Poria cocos oligosaccharides (PCO)	146:185	In this study, novel Poria cocos oligosaccharides (PCO) were prepared by enzymatic degradation, and their polymerization degree was determined to be 2-6 by LC-MS analysis.					
35671132	1	6	theme	polymerization	237:250	arg1	degree					252:257	their polymerization degree	231:257	their polymerization degree	231:257	In this study, novel Poria cocos oligosaccharides (PCO) were prepared by enzymatic degradation, and their polymerization degree was determined to be 2-6 by LC-MS analysis.					
35671132	4	7	theme	PCO	841:843	arg1	effect					831:836	the preventive effect	816:836	the preventive effect of PCO on dyslipidemia	816:859	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	3	8	theme	lipid	576:580	arg1	disorders					593:601	lipid metabolism disorders	576:601	lipid metabolism disorders	576:601	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	4	9	theme	correlation	778:788	arg1	analysis					790:797	Spearman's correlation analysis	767:797	Spearman's correlation analysis	767:797	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	5	10	theme	PCO	1147:1149	arg1	application					1132:1142	the potential application	1118:1142	the potential application of PCO for the treatment of dyslipidemia	1118:1183	Our research shed light on the potential application of PCO for the treatment of dyslipidemia.					
35671132	2	11	contain	contain	407:413	arg2	-α-D-Glcp					462:470	(1 → 2)-α-D-Glcp	455:470	(1 → 2)-α-D-Glcp	455:470	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	11	contain	contain	407:413	arg2	residues					425:432	the sugar residues	415:432	the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp	415:492	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	11	contain	contain	407:413	arg1	PCO					387:389	PCO	387:389	PCO	387:389	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	11	contain	contain	407:413	arg2	-α-D-Glcp					484:492	(1 → 4)-α-D-Glcp	477:492	(1 → 4)-α-D-Glcp	477:492	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	11	contain	contain	407:413	arg2	-β-D-Glcp					444:452	(1 → 2)-β-D-Glcp	437:452	(1 → 2)-β-D-Glcp	437:452	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	12	theme	methylation	343:353	arg1	assay					355:359	methylation assay	343:359	methylation assay	343:359	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	4	13	theme	changed	897:903	arg1	pathways					915:922	changed metabolic pathways	897:922	changed metabolic pathways	897:922	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	0	14	theme	diet-fed	116:123	arg1	mice					125:128	high-fat diet-fed mice	107:128	high-fat diet-fed mice	107:128	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	3	15	from	reduction	625:633	arg1	liver					701:705	liver	701:705	liver	701:705	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	3	15	from	reduction	625:633	arg1	blood					691:695	blood	691:695	blood	691:695	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	1	16	theme	cocos	158:162	arg1	PCO					182:184	PCO	182:184	PCO	182:184	In this study, novel Poria cocos oligosaccharides (PCO) were prepared by enzymatic degradation, and their polymerization degree was determined to be 2-6 by LC-MS analysis.					
35671132	1	16	theme	cocos	158:162	arg1	oligosaccharides					164:179	novel Poria cocos oligosaccharides	146:179	novel Poria cocos oligosaccharides (PCO)	146:185	In this study, novel Poria cocos oligosaccharides (PCO) were prepared by enzymatic degradation, and their polymerization degree was determined to be 2-6 by LC-MS analysis.					
35671132	0	17	theme	high-fat	107:114	arg1	mice					125:128	high-fat diet-fed mice	107:128	high-fat diet-fed mice	107:128	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	0	18	from	oligosaccharides	43:58	arg1	metabolome					93:102	the hepatic metabolome	81:102	the hepatic metabolome in high-fat diet-fed mice	81:128	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	4	19	theme	pathways	915:922	arg1	reversal					885:892	the reversal	881:892	the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione	881:1088	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	0	20	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.	0:129	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	3	21	theme	lipid	638:642	arg1	accumulation					644:655	lipid accumulation	638:655	lipid accumulation	638:655	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	4	22	theme	metabolomic	742:752	arg1	analysis					754:761	the non-targeted metabolomic analysis	725:761	the non-targeted metabolomic analysis	725:761	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	23	theme	non-targeted	729:740	arg1	analysis					754:761	the non-targeted metabolomic analysis	725:761	the non-targeted metabolomic analysis	725:761	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	5	24	theme	dyslipidemia	1172:1183	arg1	treatment					1159:1167	the treatment	1155:1167	the treatment of dyslipidemia	1155:1183	Our research shed light on the potential application of PCO for the treatment of dyslipidemia.					
35671132	0	25	theme	Poria	31:35	arg1	oligosaccharides					43:58	Poria cocos oligosaccharides	31:58	Poria cocos oligosaccharides	31:58	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	3	26	theme	accumulation	644:655	arg1	reduction					625:633	the reduction	621:633	the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues	621:713	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	2	27	theme	-α-D-Glcp	462:470	arg1	-α-D-Glcp					484:492	(1 → 4)-α-D-Glcp	477:492	(1 → 4)-α-D-Glcp	477:492	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	27	theme	-α-D-Glcp	462:470	arg1	residues					425:432	the sugar residues	415:432	the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp	415:492	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	27	theme	-α-D-Glcp	462:470	arg1	-α-D-Glcp					462:470	(1 → 2)-α-D-Glcp	455:470	(1 → 2)-α-D-Glcp	455:470	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	27	theme	-α-D-Glcp	462:470	arg1	-β-D-Glcp					444:452	(1 → 2)-β-D-Glcp	437:452	(1 → 2)-β-D-Glcp	437:452	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	3	28	with	model	518:522	arg1	dyslipidemia					529:540	dyslipidemia	529:540	dyslipidemia	529:540	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	4	29	from	effect	831:836	arg1	dyslipidemia					848:859	dyslipidemia	848:859	dyslipidemia	848:859	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	30	theme	amino	1013:1017	arg1	acids					1006:1010	unsaturated fatty acids	988:1010	unsaturated fatty acids	988:1010	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	30	theme	amino	1013:1017	arg1	acids					1019:1023	amino acids	1013:1023	amino acids	1013:1023	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	0	31	theme	oligosaccharides	43:58	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.	0:129	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	3	32	dep	blood	691:695	arg1	the					687:689	the	687:689	the	687:689	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	3	32	dep	blood	691:695	arg1	tissues					707:713	tissues	707:713	tissues	707:713	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	0	33	theme	cocos	37:41	arg1	oligosaccharides					43:58	Poria cocos oligosaccharides	31:58	Poria cocos oligosaccharides	31:58	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	3	34	theme	responses	674:682	arg1	reduction					625:633	the reduction	621:633	the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues	621:713	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	4	35	theme	preventive	820:829	arg1	effect					831:836	the preventive effect	816:836	the preventive effect of PCO on dyslipidemia	816:859	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	3	36	theme	mouse	512:516	arg1	model					518:522	an HFD-fed mouse model	501:522	an HFD-fed mouse model with dyslipidemia	501:540	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	2	37	theme	-β-D-Glcp	444:452	arg1	-α-D-Glcp					484:492	(1 → 4)-α-D-Glcp	477:492	(1 → 4)-α-D-Glcp	477:492	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	37	theme	-β-D-Glcp	444:452	arg1	residues					425:432	the sugar residues	415:432	the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp	415:492	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	37	theme	-β-D-Glcp	444:452	arg1	-α-D-Glcp					462:470	(1 → 2)-α-D-Glcp	455:470	(1 → 2)-α-D-Glcp	455:470	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	37	theme	-β-D-Glcp	444:452	arg1	-β-D-Glcp					444:452	(1 → 2)-β-D-Glcp	437:452	(1 → 2)-β-D-Glcp	437:452	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	4	38	theme	metabolic	905:913	arg1	pathways					915:922	changed metabolic pathways	897:922	changed metabolic pathways	897:922	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	1	39	theme	LC-MS	287:291	arg1	analysis					293:300	LC-MS analysis	287:300	LC-MS analysis	287:300	In this study, novel Poria cocos oligosaccharides (PCO) were prepared by enzymatic degradation, and their polymerization degree was determined to be 2-6 by LC-MS analysis.					
35671132	0	40	from	effects	70:76	arg1	metabolome					93:102	the hepatic metabolome	81:102	the hepatic metabolome in high-fat diet-fed mice	81:128	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	2	41	theme	sugar	419:423	arg1	-α-D-Glcp					484:492	(1 → 4)-α-D-Glcp	477:492	(1 → 4)-α-D-Glcp	477:492	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	41	theme	sugar	419:423	arg1	residues					425:432	the sugar residues	415:432	the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp	415:492	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	41	theme	sugar	419:423	arg1	-α-D-Glcp					462:470	(1 → 2)-α-D-Glcp	455:470	(1 → 2)-α-D-Glcp	455:470	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	41	theme	sugar	419:423	arg1	-β-D-Glcp					444:452	(1 → 2)-β-D-Glcp	437:452	(1 → 2)-β-D-Glcp	437:452	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	2	42	theme	composition	321:331	arg1	analysis					333:340	monosaccharide composition analysis	306:340	monosaccharide composition analysis	306:340	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	4	43	theme	glycerophospholipids	966:985	arg1	choline					1026:1032	choline	1026:1032	choline	1026:1032	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	43	theme	glycerophospholipids	966:985	arg1	sphingolipids					1059:1071	sphingolipids	1059:1071	sphingolipids	1059:1071	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	43	theme	glycerophospholipids	966:985	arg1	metabolisms					951:961	the metabolisms	947:961	the metabolisms of glycerophospholipids	947:985	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	43	theme	glycerophospholipids	966:985	arg1	acids					1006:1010	unsaturated fatty acids	988:1010	unsaturated fatty acids	988:1010	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	43	theme	glycerophospholipids	966:985	arg1	acids					1040:1044	bile acids	1035:1044	bile acids	1035:1044	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	43	theme	glycerophospholipids	966:985	arg1	glutathione					1078:1088	glutathione	1078:1088	glutathione	1078:1088	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	43	theme	glycerophospholipids	966:985	arg1	tryptophan					1047:1056	tryptophan	1047:1056	tryptophan	1047:1056	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	43	theme	glycerophospholipids	966:985	arg1	acids					1019:1023	amino acids	1013:1023	amino acids	1013:1023	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	3	44	theme	inflammatory	661:672	arg1	responses					674:682	inflammatory responses	661:682	inflammatory responses	661:682	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	2	45	theme	monosaccharide	306:319	arg1	analysis					333:340	monosaccharide composition analysis	306:340	monosaccharide composition analysis	306:340	By monosaccharide composition analysis, methylation assay, FT-IR, and NMR analysis, PCO were deduced to contain the sugar residues of (1 → 2)-β-D-Glcp, (1 → 2)-α-D-Glcp, and (1 → 4)-α-D-Glcp.					
35671132	1	46	theme	enzymatic	204:212	arg1	degradation					214:224	enzymatic degradation	204:224	enzymatic degradation	204:224	In this study, novel Poria cocos oligosaccharides (PCO) were prepared by enzymatic degradation, and their polymerization degree was determined to be 2-6 by LC-MS analysis.					
35671132	3	47	theme	HFD-fed	504:510	arg1	model					518:522	an HFD-fed mouse model	501:522	an HFD-fed mouse model with dyslipidemia	501:540	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	4	48	theme	fatty	1000:1004	arg1	choline					1026:1032	choline	1026:1032	choline	1026:1032	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	48	theme	fatty	1000:1004	arg1	acids					1006:1010	unsaturated fatty acids	988:1010	unsaturated fatty acids	988:1010	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	48	theme	fatty	1000:1004	arg1	acids					1019:1023	amino acids	1013:1023	amino acids	1013:1023	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	48	theme	fatty	1000:1004	arg1	acids					1040:1044	bile acids	1035:1044	bile acids	1035:1044	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	49	theme	unsaturated	988:998	arg1	choline					1026:1032	choline	1026:1032	choline	1026:1032	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	49	theme	unsaturated	988:998	arg1	acids					1006:1010	unsaturated fatty acids	988:1010	unsaturated fatty acids	988:1010	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	49	theme	unsaturated	988:998	arg1	acids					1019:1023	amino acids	1013:1023	amino acids	1013:1023	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	49	theme	unsaturated	988:998	arg1	acids					1040:1044	bile acids	1035:1044	bile acids	1035:1044	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	0	50	from	metabolome	93:102	arg1	mice					125:128	high-fat diet-fed mice	107:128	high-fat diet-fed mice	107:128	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	0	50	from	metabolome	93:102	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.	0:129	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	4	51	theme	bile	1035:1038	arg1	acids					1040:1044	bile acids	1035:1044	bile acids	1035:1044	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	4	51	theme	bile	1035:1038	arg1	acids					1006:1010	unsaturated fatty acids	988:1010	unsaturated fatty acids	988:1010	Based on the non-targeted metabolomic analysis and Spearman's correlation analysis, we presume that the preventive effect of PCO on dyslipidemia might contribute to the reversal of changed metabolic pathways, which were related to the metabolisms of glycerophospholipids, unsaturated fatty acids, amino acids, choline, bile acids, tryptophan, sphingolipids, and glutathione.					
35671132	0	52	theme	effects	70:76	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.	0:129	Structural characterization of Poria cocos oligosaccharides and their effects on the hepatic metabolome in high-fat diet-fed mice.					
35671132	3	53	theme	metabolism	582:591	arg1	disorders					593:601	lipid metabolism disorders	576:601	lipid metabolism disorders	576:601	Using an HFD-fed mouse model with dyslipidemia, PCO could significantly suppress lipid metabolism disorders, characterized by the reduction of lipid accumulation and inflammatory responses in the blood and liver tissues.					
35671132	5	54	theme	potential	1122:1130	arg1	application					1132:1142	the potential application	1118:1142	the potential application of PCO for the treatment of dyslipidemia	1118:1183	Our research shed light on the potential application of PCO for the treatment of dyslipidemia.					
35550762	0	0	theme	Comparative	84:94	arg1	study					96:100	Comparative study	84:100	Comparative study between different processes	84:128	Lignin containing micro and nano-fibrillated cellulose obtained by steam explosion: Comparative study between different processes.					
35550762	7	1	with	pulps	788:792	arg1	content					813:819	higher lignin content	799:819	higher lignin content	799:819	Results showed that SE can replace SC for the production of pulps with higher lignin content: 8-12% wt.					
35550762	1	2	theme	micro-	166:171	arg1	isolation					135:143	The isolation	131:143	The isolation of lignin containing micro- and nanofibrillated cellulose (L-MNFC)	131:210	The isolation of lignin containing micro- and nanofibrillated cellulose (L-MNFC) requires a multistep process.					
35550762	7	3	theme	higher	799:804	arg1	content					813:819	higher lignin content	799:819	higher lignin content	799:819	Results showed that SE can replace SC for the production of pulps with higher lignin content: 8-12% wt.					
35550762	3	4	theme	control	447:453	arg1	sample					455:460	a control sample	445:460	a control sample	445:460	A conventional cooking in an autoclave (SC) was applied in order to get a control sample.					
35550762	5	5	theme	processes	636:644	arg1	effect					612:617	the effect	608:617	the effect of the different processes	608:644	The chemical composition of the pulps was assessed as well as fiber morphology to compare the effect of the different processes.					
35550762	3	6	from	cooking	388:394	arg1	autoclave					402:410	an autoclave	399:410	an autoclave (SC)	399:415	A conventional cooking in an autoclave (SC) was applied in order to get a control sample.					
35550762	3	6	from	cooking	388:394	arg1	SC					413:414	SC	413:414	SC	413:414	A conventional cooking in an autoclave (SC) was applied in order to get a control sample.					
35550762	5	7	theme	chemical	522:529	arg1	morphology					586:595	fiber morphology	580:595	fiber morphology	580:595	The chemical composition of the pulps was assessed as well as fiber morphology to compare the effect of the different processes.					
35550762	5	7	theme	chemical	522:529	arg1	composition					531:541	The chemical composition	518:541	The chemical composition of the pulps	518:554	The chemical composition of the pulps was assessed as well as fiber morphology to compare the effect of the different processes.					
35550762	2	8	theme	steam	287:291	arg1	SE					304:305	SE	304:305	SE	304:305	In this study, beech wood was pre-treated by steam explosion (SE), refined or pre-treated by 3 SE and grinded until gel formation.					
35550762	2	8	theme	steam	287:291	arg1	explosion					293:301	steam explosion	287:301	steam explosion (SE)	287:306	In this study, beech wood was pre-treated by steam explosion (SE), refined or pre-treated by 3 SE and grinded until gel formation.					
35550762	7	9	theme	pulps	788:792	arg1	production					774:783	the production	770:783	the production of pulps with higher lignin content: 8-12% wt	770:829	Results showed that SE can replace SC for the production of pulps with higher lignin content: 8-12% wt.					
35550762	5	10	theme	fiber	580:584	arg1	morphology					586:595	fiber morphology	580:595	fiber morphology	580:595	The chemical composition of the pulps was assessed as well as fiber morphology to compare the effect of the different processes.					
35550762	5	10	theme	fiber	580:584	arg1	composition					531:541	The chemical composition	518:541	The chemical composition of the pulps	518:554	The chemical composition of the pulps was assessed as well as fiber morphology to compare the effect of the different processes.					
35550762	9	11	theme	nanoparticles	955:967	arg1	dispersion					934:943	a dispersion	932:943	a dispersion of lignin nanoparticles and some nanocrystals	932:989	Gels contain micro and nanofibrillated cellulose and residual fibers with a dispersion of lignin nanoparticles and some nanocrystals.					
35550762	1	12	theme	nanofibrillated	177:191	arg1	L-MNFC					204:209	L-MNFC	204:209	L-MNFC	204:209	The isolation of lignin containing micro- and nanofibrillated cellulose (L-MNFC) requires a multistep process.					
35550762	1	12	theme	nanofibrillated	177:191	arg1	cellulose					193:201	nanofibrillated cellulose	177:201	nanofibrillated cellulose (L-MNFC)	177:210	The isolation of lignin containing micro- and nanofibrillated cellulose (L-MNFC) requires a multistep process.					
35550762	0	13	theme	different	110:118	arg1	processes					120:128	different processes	110:128	different processes	110:128	Lignin containing micro and nano-fibrillated cellulose obtained by steam explosion: Comparative study between different processes.					
35550762	5	14	theme	pulps	550:554	arg1	morphology					586:595	fiber morphology	580:595	fiber morphology	580:595	The chemical composition of the pulps was assessed as well as fiber morphology to compare the effect of the different processes.					
35550762	5	14	theme	pulps	550:554	arg1	composition					531:541	The chemical composition	518:541	The chemical composition of the pulps	518:554	The chemical composition of the pulps was assessed as well as fiber morphology to compare the effect of the different processes.					
35550762	1	15	theme	cellulose	193:201	arg1	isolation					135:143	The isolation	131:143	The isolation of lignin containing micro- and nanofibrillated cellulose (L-MNFC)	131:210	The isolation of lignin containing micro- and nanofibrillated cellulose (L-MNFC) requires a multistep process.					
35550762	10	16	theme	ultrafine	1012:1020	arg1	step					1031:1034	the ultrafine grinding step	1008:1034	the ultrafine grinding step	1008:1034	Optimization of the ultrafine grinding step was required to minimize the energy consumption.					
35550762	7	17	dep	pulps	788:792	arg1	wt					828:829	 8-12% wt	821:829	pulps with higher lignin content: 8-12% wt	788:829	Results showed that SE can replace SC for the production of pulps with higher lignin content: 8-12% wt.					
35550762	9	18	theme	nanocrystals	978:989	arg1	dispersion					934:943	a dispersion	932:943	a dispersion of lignin nanoparticles and some nanocrystals	932:989	Gels contain micro and nanofibrillated cellulose and residual fibers with a dispersion of lignin nanoparticles and some nanocrystals.					
35550762	6	19	theme	production	695:704	arg1	process					706:712	the production process	691:712	the production process	691:712	Quality index and energy consumption during the production process were studied.					
35550762	7	20	theme	%	826:826	arg1	wt					828:829	 8-12% wt	821:829	pulps with higher lignin content: 8-12% wt	788:829	Results showed that SE can replace SC for the production of pulps with higher lignin content: 8-12% wt.					
35550762	0	21	theme	micro	18:22	arg1	cellulose					45:53	micro and nano-fibrillated cellulose	18:53	cellulose	45:53	Lignin containing micro and nano-fibrillated cellulose obtained by steam explosion: Comparative study between different processes.					
35550762	4	22	theme	pulp	495:498	arg1	bleaching					477:485	bleaching	477:485	bleaching of a SE pulp	477:498	The effect of bleaching of a SE pulp was also studied.					
35550762	9	23	theme	residual	911:918	arg1	fibers					920:925	nanofibrillated cellulose and residual fibers	881:925	nanofibrillated cellulose and residual fibers	881:925	Gels contain micro and nanofibrillated cellulose and residual fibers with a dispersion of lignin nanoparticles and some nanocrystals.					
35550762	3	24	theme	conventional	375:386	arg1	cooking					388:394	A conventional cooking	373:394	A conventional cooking in an autoclave (SC)	373:415	A conventional cooking in an autoclave (SC) was applied in order to get a control sample.					
35550762	4	25	theme	SE	492:493	arg1	pulp					495:498	a SE pulp	490:498	a SE pulp	490:498	The effect of bleaching of a SE pulp was also studied.					
35550762	9	26	contain	contain	863:869	arg1	Gels					858:861	Gels	858:861	Gels	858:861	Gels contain micro and nanofibrillated cellulose and residual fibers with a dispersion of lignin nanoparticles and some nanocrystals.					
35550762	9	26	contain	contain	863:869	arg2	micro					871:875	micro	871:875	micro	871:875	Gels contain micro and nanofibrillated cellulose and residual fibers with a dispersion of lignin nanoparticles and some nanocrystals.					
35550762	9	26	contain	contain	863:869	arg2	fibers					920:925	nanofibrillated cellulose and residual fibers	881:925	nanofibrillated cellulose and residual fibers	881:925	Gels contain micro and nanofibrillated cellulose and residual fibers with a dispersion of lignin nanoparticles and some nanocrystals.					
35550762	0	27	theme	nano-fibrillated	28:43	arg1	cellulose					45:53	micro and nano-fibrillated cellulose	18:53	cellulose	45:53	Lignin containing micro and nano-fibrillated cellulose obtained by steam explosion: Comparative study between different processes.					
35550762	5	28	theme	different	626:634	arg1	processes					636:644	the different processes	622:644	the different processes	622:644	The chemical composition of the pulps was assessed as well as fiber morphology to compare the effect of the different processes.					
35550762	1	29	theme	multistep	223:231	arg1	process					233:239	a multistep process	221:239	a multistep process	221:239	The isolation of lignin containing micro- and nanofibrillated cellulose (L-MNFC) requires a multistep process.					
35550762	9	30	theme	nanofibrillated	881:895	arg1	fibers					920:925	nanofibrillated cellulose and residual fibers	881:925	nanofibrillated cellulose and residual fibers	881:925	Gels contain micro and nanofibrillated cellulose and residual fibers with a dispersion of lignin nanoparticles and some nanocrystals.					
35550762	10	31	theme	step	1031:1034	arg1	Optimization					992:1003	Optimization	992:1003	Optimization of the ultrafine grinding step	992:1034	Optimization of the ultrafine grinding step was required to minimize the energy consumption.					
35550762	9	32	theme	cellulose	897:905	arg1	fibers					920:925	nanofibrillated cellulose and residual fibers	881:925	nanofibrillated cellulose and residual fibers	881:925	Gels contain micro and nanofibrillated cellulose and residual fibers with a dispersion of lignin nanoparticles and some nanocrystals.					
35550762	4	33	theme	bleaching	477:485	arg1	effect					467:472	The effect	463:472	The effect of bleaching of a SE pulp	463:498	The effect of bleaching of a SE pulp was also studied.					
35550762	6	34	theme	energy	665:670	arg1	consumption					672:682	energy consumption	665:682	energy consumption	665:682	Quality index and energy consumption during the production process were studied.					
35550762	7	35	theme	 8-12	821:825	arg1	%					826:826	%	826:826	%	826:826	Results showed that SE can replace SC for the production of pulps with higher lignin content: 8-12% wt.					
35550762	10	36	theme	grinding	1022:1029	arg1	step					1031:1034	the ultrafine grinding step	1008:1034	the ultrafine grinding step	1008:1034	Optimization of the ultrafine grinding step was required to minimize the energy consumption.					
35550762	2	37	theme	beech	257:261	arg1	wood					263:266	beech wood	257:266	beech wood	257:266	In this study, beech wood was pre-treated by steam explosion (SE), refined or pre-treated by 3 SE and grinded until gel formation.					
35550762	7	38	theme	lignin	806:811	arg1	content					813:819	higher lignin content	799:819	higher lignin content	799:819	Results showed that SE can replace SC for the production of pulps with higher lignin content: 8-12% wt.					
35550762	9	39	theme	lignin	948:953	arg1	nanoparticles					955:967	lignin nanoparticles	948:967	lignin nanoparticles	948:967	Gels contain micro and nanofibrillated cellulose and residual fibers with a dispersion of lignin nanoparticles and some nanocrystals.					
35550762	0	40	dep	containing	7:16	arg1	study					96:100	Comparative study	84:100	Comparative study between different processes	84:128	Lignin containing micro and nano-fibrillated cellulose obtained by steam explosion: Comparative study between different processes.					
35550762	6	41	theme	Quality	647:653	arg1	index					655:659	Quality index	647:659	Quality index	647:659	Quality index and energy consumption during the production process were studied.					
35550762	1	42	theme	lignin	148:153	arg1	micro-					166:171	lignin containing micro-	148:171	lignin containing micro-	148:171	The isolation of lignin containing micro- and nanofibrillated cellulose (L-MNFC) requires a multistep process.					
35550762	2	43	theme	gel	358:360	arg1	formation					362:370	gel formation	358:370	gel formation	358:370	In this study, beech wood was pre-treated by steam explosion (SE), refined or pre-treated by 3 SE and grinded until gel formation.					
35550762	0	44	theme	steam	67:71	arg1	explosion					73:81	steam explosion	67:81	steam explosion	67:81	Lignin containing micro and nano-fibrillated cellulose obtained by steam explosion: Comparative study between different processes.					
35550762	1	45	theme	containing	155:164	arg1	micro-					166:171	lignin containing micro-	148:171	lignin containing micro-	148:171	The isolation of lignin containing micro- and nanofibrillated cellulose (L-MNFC) requires a multistep process.					
35550762	10	46	theme	energy	1065:1070	arg1	consumption					1072:1082	the energy consumption	1061:1082	the energy consumption	1061:1082	Optimization of the ultrafine grinding step was required to minimize the energy consumption.					
35485965	6	0	theme	proper	1699:1704	arg1	growth					1711:1716	proper body growth	1699:1716	proper body growth	1699:1716	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	7	1	attach	present	1836:1842	arg2	maize					1811:1815	the high maize and millet content	1802:1834	maize	1811:1815	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	7	1	attach	present	1836:1842	arg1	them					1847:1850	them	1847:1850	them	1847:1850	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	6	2	theme	more	1566:1569	arg1	emphasis					1571:1578	more emphasis	1566:1578	more emphasis	1566:1578	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	7	3	from	present	1836:1842	arg1	them					1847:1850	them	1847:1850	them	1847:1850	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	2	4	theme	moringa	553:559	arg1	amount					476:481	the amount	472:481	the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn	472:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	4	theme	moringa	553:559	arg1	moringa					553:559	moringa	553:559	moringa leaves while maintaining a constant amount of yellow corn	553:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	4	theme	moringa	553:559	arg1	peels					543:547	measured acha flour, soybean flour, and blends of orange peels	486:547	measured acha flour, soybean flour, and blends of orange peels	486:547	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	3	5	theme	functional	685:694	arg1	cereals					696:702	functional cereals	685:702	functional cereals	685:702	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	1	6	from	index	279:283	arg1	enzymes					354:360	α-amylase and α-glucosidase enzymes	326:360	α-amylase and α-glucosidase enzymes	326:360	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	3	7	dep	peels	640:644	arg1	blends					665:670	blends	665:670	blends	665:670	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	7	8	from	them	1847:1850	arg1	present					1836:1842	present	1836:1842	present	1836:1842	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	4	9	theme	starch	1250:1255	arg1	content					1257:1263	starch content	1250:1263	starch content	1250:1263	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	5	10	dep	APPLICATIONS	1348:1359	arg1	consumed					1394:1401	consumed	1394:1401	are highly consumed by children and by adults inclusively	1383:1439	PRACTICAL APPLICATIONS: Ready-to-eat cereals are highly consumed by children and by adults inclusively.					
35485965	1	11	theme	orange	215:220	arg1	peels					222:226	orange peels	215:226	orange peels	215:226	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	3	12	contain	had	704:706	arg2	constituents					741:752	significantly increased phenolic constituents	708:752	significantly increased phenolic constituents	708:752	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	3	12	contain	had	704:706	arg1	inclusion					672:680	The produced orange peels and moringa leaves blends inclusion	620:680	The produced orange peels and moringa leaves blends inclusion to functional cereals	620:702	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	2	13	dep	moringa	553:559	arg1	leaves					561:566	leaves	561:566	leaves while maintaining a constant amount of yellow corn	561:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	14	theme	flour	515:519	arg1	peels					543:547	measured acha flour, soybean flour, and blends of orange peels	486:547	measured acha flour, soybean flour, and blends of orange peels	486:547	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	0	15	from	role	70:73	arg1	indices					87:93	glycemic indices	78:93	glycemic indices in-vitro	78:102	Functional cereals' anti-diabetic property, phenolic composition, and role on glycemic indices in-vitro.					
35485965	2	16	theme	peels	543:547	arg1	amount					476:481	the amount	472:481	the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn	472:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	16	theme	peels	543:547	arg1	moringa					553:559	moringa	553:559	moringa leaves while maintaining a constant amount of yellow corn	553:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	16	theme	peels	543:547	arg1	peels					543:547	measured acha flour, soybean flour, and blends of orange peels	486:547	measured acha flour, soybean flour, and blends of orange peels	486:547	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	3	17	dep	moringa	650:656	arg1	leaves					658:663	leaves	658:663	leaves	658:663	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	3	18	theme	α-amylase	785:793	arg1	enzymes					813:819	α-amylase and α-glucosidase enzymes	785:819	α-amylase and α-glucosidase enzymes in-vitro	785:828	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	2	19	with	proportion	442:451	arg1	variations					458:467	variations	458:467	variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn	458:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	8	20	from	nutritious	2038:2047	arg1	nature					2071:2076	nature	2071:2076	nature	2071:2076	This study shows that functional cereals produced in-vitro extensively are not only nutritious but also medicinal in nature, offering benefits in the management of diabetes and related complications.					
35485965	4	21	theme	best	961:964	arg1	cereals					970:976	the best two cereals	957:976	the best two cereals	957:976	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	6	22	theme	high	1538:1541	arg1	constituent					1551:1561	high calorie constituent	1538:1561	high calorie constituent	1538:1561	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	7	23	theme	diabetic	1935:1942	arg1	patients					1944:1951	diabetic patients	1935:1951	diabetic patients	1935:1951	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	4	24	theme	golden	1324:1329	arg1	cereals					1315:1321	commercially consumed cereals	1293:1321	commercially consumed cereals (golden morn)	1293:1335	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	24	theme	golden	1324:1329	arg1	morn					1331:1334	golden morn	1324:1334	golden morn	1324:1334	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	6	25	theme	vitamins	1630:1637	arg1	A					1639:1639	vitamins A	1630:1639	vitamins A	1630:1639	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	4	26	theme	high	1161:1164	arg1	effects					1177:1183	the high inhibitory effects	1157:1183	the high inhibitory effects on the activities of α-amylase and α-glucosidase	1157:1232	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	3	27	theme	in-vitro	821:828	arg1	enzymes					813:819	α-amylase and α-glucosidase enzymes	785:819	α-amylase and α-glucosidase enzymes in-vitro	785:828	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	1	28	theme	cereals	151:157	arg1	effects					129:135	the effects	125:135	the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro	125:369	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	3	29	theme	starch	858:863	arg1	content					865:871	starch content	858:871	starch content	858:871	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	4	30	theme	functional	937:946	arg1	cereals					948:954	the produced functional cereals	924:954	the produced functional cereals	924:954	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	5	31	theme	Ready-to-eat	1362:1373	arg1	cereals					1375:1381	Ready-to-eat cereals	1362:1381	Ready-to-eat cereals	1362:1381	PRACTICAL APPLICATIONS: Ready-to-eat cereals are highly consumed by children and by adults inclusively.					
35485965	2	32	theme	corn	614:617	arg1	corn					614:617	yellow corn	607:617	yellow corn	607:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	32	theme	corn	614:617	arg1	amount					597:602	a constant amount	586:602	a constant amount of yellow corn	586:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	3	33	theme	α-glucosidase	799:811	arg1	enzymes					813:819	α-amylase and α-glucosidase enzymes	785:819	α-amylase and α-glucosidase enzymes in-vitro	785:828	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	0	34	theme	glycemic	78:85	arg1	indices					87:93	glycemic indices	78:93	glycemic indices in-vitro	78:102	Functional cereals' anti-diabetic property, phenolic composition, and role on glycemic indices in-vitro.					
35485965	2	35	theme	acha	495:498	arg1	flour					500:504	acha flour	495:504	acha flour	495:504	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	6	36	theme	nutrients	1599:1607	arg1	value					1609:1613	their nutrients value	1593:1613	their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning	1593:1732	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	4	37	from	effects	1177:1183	arg1	activities					1192:1201	the activities	1188:1201	the activities of α-amylase and α-glucosidase	1188:1232	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	0	38	theme	in-vitro	95:102	arg1	indices					87:93	glycemic indices	78:93	glycemic indices in-vitro	78:102	Functional cereals' anti-diabetic property, phenolic composition, and role on glycemic indices in-vitro.					
35485965	8	39	from	benefits	2088:2095	arg1	management					2104:2113	the management	2100:2113	the management of diabetes and related complications	2100:2151	This study shows that functional cereals produced in-vitro extensively are not only nutritious but also medicinal in nature, offering benefits in the management of diabetes and related complications.					
35485965	0	40	from	property	34:41	arg1	indices					87:93	glycemic indices	78:93	glycemic indices in-vitro	78:102	Functional cereals' anti-diabetic property, phenolic composition, and role on glycemic indices in-vitro.					
35485965	0	41	theme	Functional	0:9	arg1	cereals					11:17	Functional cereals	0:17	Functional cereals	0:17	Functional cereals' anti-diabetic property, phenolic composition, and role on glycemic indices in-vitro.					
35485965	6	42	dep	fact	1526:1529	arg1	has					1534:1536	has	1534:1536	has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning	1534:1732	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	2	43	theme	ready-to-eat	408:419	arg1	cereals					421:427	ready-to-eat cereals	408:427	ready-to-eat cereals	408:427	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	43	theme	ready-to-eat	408:419	arg1	cereals					383:389	Functional cereals	372:389	Functional cereals	372:389	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	8	44	theme	complications	2139:2151	arg1	management					2104:2113	the management	2100:2113	the management of diabetes and related complications	2100:2151	This study shows that functional cereals produced in-vitro extensively are not only nutritious but also medicinal in nature, offering benefits in the management of diabetes and related complications.					
35485965	1	45	from	effects	315:321	arg1	enzymes					354:360	α-amylase and α-glucosidase enzymes	326:360	α-amylase and α-glucosidase enzymes	326:360	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	1	46	theme	glycemic	270:277	arg1	index					279:283	glycemic index	270:283	glycemic index	270:283	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	7	47	theme	breakfast	1761:1769	arg1	cereals					1771:1777	these commercial breakfast cereals	1744:1777	these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them	1744:1850	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	7	47	theme	breakfast	1761:1769	arg1	morn					1787:1790	golden morn	1780:1790	golden morn	1780:1790	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	7	48	with	consumers	1879:1887	arg1	patients					1944:1951	diabetic patients	1935:1951	diabetic patients	1935:1951	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	7	48	with	consumers	1879:1887	arg1	challenges					1916:1925	health challenges	1909:1925	health challenges such as diabetic patients	1909:1951	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	0	49	theme	phenolic	44:51	arg1	composition					53:63	phenolic composition	44:63	phenolic composition	44:63	Functional cereals' anti-diabetic property, phenolic composition, and role on glycemic indices in-vitro.					
35485965	0	50	dep	property	34:41	arg1	cereals					11:17	Functional cereals	0:17	Functional cereals	0:17	Functional cereals' anti-diabetic property, phenolic composition, and role on glycemic indices in-vitro.					
35485965	4	51	theme	orange	1038:1043	arg1	peel					1045:1048	orange peel	1038:1048	orange peel	1038:1048	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	51	theme	orange	1038:1043	arg1	corn					1017:1020	corn	1017:1020	corn	1017:1020	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	3	52	theme	peels	640:644	arg1	inclusion					672:680	The produced orange peels and moringa leaves blends inclusion	620:680	The produced orange peels and moringa leaves blends inclusion to functional cereals	620:702	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	7	53	with	individuals	1892:1902	arg1	patients					1944:1951	diabetic patients	1935:1951	diabetic patients	1935:1951	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	7	53	with	individuals	1892:1902	arg1	challenges					1916:1925	health challenges	1909:1925	health challenges such as diabetic patients	1909:1951	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	4	54	dep	moringa	1112:1118	arg1	leaves					1120:1125	leaves	1120:1125	leaves	1120:1125	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	3	55	theme	produced	624:631	arg1	inclusion					672:680	The produced orange peels and moringa leaves blends inclusion	620:680	The produced orange peels and moringa leaves blends inclusion to functional cereals	620:702	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	6	56	theme	body	1706:1709	arg1	growth					1711:1716	proper body growth	1699:1716	proper body growth	1699:1716	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	6	57	contain	has	1534:1536	arg1	it					1531:1532	it	1531:1532	it	1531:1532	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	6	57	contain	has	1534:1536	arg2	constituent					1551:1561	high calorie constituent	1538:1561	high calorie constituent	1538:1561	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	3	58	theme	moringa	650:656	arg1	inclusion					672:680	The produced orange peels and moringa leaves blends inclusion	620:680	The produced orange peels and moringa leaves blends inclusion to functional cereals	620:702	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	1	59	from	content	261:267	arg1	enzymes					354:360	α-amylase and α-glucosidase enzymes	326:360	α-amylase and α-glucosidase enzymes	326:360	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	7	60	theme	certain	1871:1877	arg1	consumers					1879:1887	certain consumers	1871:1887	certain consumers	1871:1887	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	2	61	theme	constant	588:595	arg1	corn					614:617	yellow corn	607:617	yellow corn	607:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	61	theme	constant	588:595	arg1	amount					597:602	a constant amount	586:602	a constant amount of yellow corn	586:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	4	62	theme	inhibitory	1166:1175	arg1	effects					1177:1183	the high inhibitory effects	1157:1183	the high inhibitory effects on the activities of α-amylase and α-glucosidase	1157:1232	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	3	63	theme	increased	722:730	arg1	constituents					741:752	significantly increased phenolic constituents	708:752	significantly increased phenolic constituents	708:752	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	6	64	theme	breakfast	1453:1461	arg1	cereals					1463:1469	Commercial breakfast cereals	1442:1469	Commercial breakfast cereals	1442:1469	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	3	65	theme	phenolic	732:739	arg1	constituents					741:752	significantly increased phenolic constituents	708:752	significantly increased phenolic constituents	708:752	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	1	66	theme	yellow	187:192	arg1	corn					194:197	yellow corn	187:197	yellow corn	187:197	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	4	67	contain	had	1153:1155	arg2	corn					1017:1020	corn	1017:1020	corn	1017:1020	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	67	contain	had	1153:1155	arg2	moringa					1051:1057	moringa leaves	1051:1064	moringa leaves (30: 5:15: 0.5: 0.5)	1051:1085	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	67	contain	had	1153:1155	arg2	effects					1177:1183	the high inhibitory effects	1157:1183	the high inhibitory effects on the activities of α-amylase and α-glucosidase	1157:1232	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	67	contain	had	1153:1155	arg2	soybean					1029:1035	soybean	1029:1035	soybean	1029:1035	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	67	contain	had	1153:1155	arg2	peel					1045:1048	orange peel	1038:1048	orange peel	1038:1048	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	67	contain	had	1153:1155	arg2	soybean					1103:1109	soybean	1103:1109	soybean	1103:1109	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	67	contain	had	1153:1155	arg2	corn					1091:1094	corn	1091:1094	corn	1091:1094	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	67	contain	had	1153:1155	arg2	acha					1023:1026	acha	1023:1026	acha	1023:1026	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	67	contain	had	1153:1155	arg2	moringa					1112:1118	moringa leaves	1112:1125	moringa leaves	1112:1125	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	67	contain	had	1153:1155	arg2	acha					1097:1100	acha	1097:1100	acha	1097:1100	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	67	contain	had	1153:1155	arg1	both					1148:1151	both	1148:1151	both	1148:1151	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	7	68	theme	present	1836:1842	arg1	maize					1811:1815	the high maize and millet content	1802:1834	maize	1811:1815	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	2	69	theme	soybean	507:513	arg1	flour					515:519	soybean flour	507:519	soybean flour	507:519	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	4	70	theme	α-amylase	1206:1214	arg1	activities					1192:1201	the activities	1188:1201	the activities of α-amylase and α-glucosidase	1188:1232	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	7	71	theme	millet	1821:1826	arg1	content					1828:1834	the high maize and millet content	1802:1834	content	1828:1834	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	2	72	theme	orange	536:541	arg1	flour					500:504	acha flour	495:504	acha flour	495:504	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	72	theme	orange	536:541	arg1	flour					515:519	soybean flour	507:519	soybean flour	507:519	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	72	theme	orange	536:541	arg1	blends					526:531	blends	526:531	blends of orange	526:541	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	4	73	theme	α-glucosidase	1220:1232	arg1	activities					1192:1201	the activities	1188:1201	the activities of α-amylase and α-glucosidase	1188:1232	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	74	dep	moringa	1051:1057	arg1	leaves					1059:1064	leaves	1059:1064	leaves	1059:1064	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	2	75	theme	blends	526:531	arg1	peels					543:547	measured acha flour, soybean flour, and blends of orange peels	486:547	measured acha flour, soybean flour, and blends of orange peels	486:547	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	6	76	theme	calorie	1543:1549	arg1	constituent					1551:1561	high calorie constituent	1538:1561	high calorie constituent	1538:1561	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	4	77	theme	consumed	1306:1313	arg1	cereals					1315:1321	commercially consumed cereals	1293:1321	commercially consumed cereals (golden morn)	1293:1335	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	4	77	theme	consumed	1306:1313	arg1	morn					1331:1334	golden morn	1324:1334	golden morn	1324:1334	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	6	78	theme	Commercial	1442:1451	arg1	cereals					1463:1469	Commercial breakfast cereals	1442:1469	Commercial breakfast cereals	1442:1469	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	3	79	theme	glycemic	842:849	arg1	index					851:855	glycemic index	842:855	glycemic index	842:855	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	8	80	theme	diabetes	2118:2125	arg1	management					2104:2113	the management	2100:2113	the management of diabetes and related complications	2100:2151	This study shows that functional cereals produced in-vitro extensively are not only nutritious but also medicinal in nature, offering benefits in the management of diabetes and related complications.					
35485965	4	81	theme	produced	928:935	arg1	cereals					948:954	the produced functional cereals	924:954	the produced functional cereals	924:954	Interestingly, amongst the produced functional cereals, the best two cereals were with their composition made up of corn, acha, soybean, orange peel, moringa leaves (30: 5:15: 0.5: 0.5) and corn, acha, soybean, moringa leaves (30: 5: 15: 1) which both had the high inhibitory effects on the activities of α-amylase and α-glucosidase while depleting starch content in-vitro when compared with commercially consumed cereals (golden morn).					
35485965	7	82	theme	high	1806:1809	arg1	maize					1811:1815	the high maize and millet content	1802:1834	maize	1811:1815	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	1	83	theme	functional	140:149	arg1	cereals					151:157	functional cereals	140:157	functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro	140:369	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	8	84	from	medicinal	2058:2066	arg1	nature					2071:2076	nature	2071:2076	nature	2071:2076	This study shows that functional cereals produced in-vitro extensively are not only nutritious but also medicinal in nature, offering benefits in the management of diabetes and related complications.					
35485965	2	85	theme	flour	500:504	arg1	peels					543:547	measured acha flour, soybean flour, and blends of orange peels	486:547	measured acha flour, soybean flour, and blends of orange peels	486:547	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	0	86	from	composition	53:63	arg1	indices					87:93	glycemic indices	78:93	glycemic indices in-vitro	78:102	Functional cereals' anti-diabetic property, phenolic composition, and role on glycemic indices in-vitro.					
35485965	2	87	theme	yellow	607:612	arg1	corn					614:617	yellow corn	607:617	yellow corn	607:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	1	88	theme	α-amylase	326:334	arg1	enzymes					354:360	α-amylase and α-glucosidase enzymes	326:360	α-amylase and α-glucosidase enzymes	326:360	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	2	89	theme	measured	486:493	arg1	peels					543:547	measured acha flour, soybean flour, and blends of orange peels	486:547	measured acha flour, soybean flour, and blends of orange peels	486:547	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	90	from	variations	458:467	arg1	amount					476:481	the amount	472:481	the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn	472:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	90	from	variations	458:467	arg1	moringa					553:559	moringa	553:559	moringa leaves while maintaining a constant amount of yellow corn	553:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	90	from	variations	458:467	arg1	peels					543:547	measured acha flour, soybean flour, and blends of orange peels	486:547	measured acha flour, soybean flour, and blends of orange peels	486:547	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	1	91	theme	α-glucosidase	340:352	arg1	enzymes					354:360	α-amylase and α-glucosidase enzymes	326:360	α-amylase and α-glucosidase enzymes	326:360	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	7	92	theme	golden	1780:1785	arg1	cereals					1771:1777	these commercial breakfast cereals	1744:1777	these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them	1744:1850	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	7	92	theme	golden	1780:1785	arg1	morn					1787:1790	golden morn	1780:1790	golden morn	1780:1790	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	3	93	theme	sugar	887:891	arg1	ratios					893:898	sugar ratios	887:898	sugar ratios	887:898	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	6	94	theme	immune	1677:1682	arg1	system					1684:1689	immune system	1677:1689	immune system	1677:1689	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	5	95	theme	PRACTICAL	1338:1346	arg1	APPLICATIONS					1348:1359	PRACTICAL APPLICATIONS	1338:1359	PRACTICAL APPLICATIONS: Ready-to-eat cereals are highly consumed by children and by adults inclusively.	1338:1440	PRACTICAL APPLICATIONS: Ready-to-eat cereals are highly consumed by children and by adults inclusively.					
35485965	1	96	dep	cereals	151:157	arg1	produced					173:180	produced	173:180	produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro	173:369	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	1	96	dep	cereals	151:157	arg1	processed					159:167	processed	159:167	processed	159:167	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	0	97	theme	anti-diabetic	20:32	arg1	property					34:41	anti-diabetic property	20:41	anti-diabetic property	20:41	Functional cereals' anti-diabetic property, phenolic composition, and role on glycemic indices in-vitro.					
35485965	1	98	theme	polyphenolic	248:259	arg1	content					261:267	polyphenolic content	248:267	polyphenolic content	248:267	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	7	99	theme	commercial	1750:1759	arg1	cereals					1771:1777	these commercial breakfast cereals	1744:1777	these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them	1744:1850	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	7	99	theme	commercial	1750:1759	arg1	morn					1787:1790	golden morn	1780:1790	golden morn	1780:1790	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	8	100	theme	related	2131:2137	arg1	complications					2139:2151	related complications	2131:2151	related complications	2131:2151	This study shows that functional cereals produced in-vitro extensively are not only nutritious but also medicinal in nature, offering benefits in the management of diabetes and related complications.					
35485965	2	101	theme	Functional	372:381	arg1	cereals					421:427	ready-to-eat cereals	408:427	ready-to-eat cereals	408:427	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	2	101	theme	Functional	372:381	arg1	cereals					383:389	Functional cereals	372:389	Functional cereals	372:389	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	1	102	from	composition	293:303	arg1	enzymes					354:360	α-amylase and α-glucosidase enzymes	326:360	α-amylase and α-glucosidase enzymes	326:360	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	8	103	theme	functional	1976:1985	arg1	cereals					1987:1993	functional cereals	1976:1993	functional cereals produced in-vitro	1976:2011	This study shows that functional cereals produced in-vitro extensively are not only nutritious but also medicinal in nature, offering benefits in the management of diabetes and related complications.					
35485965	8	103	theme	functional	1976:1985	arg1	nutritious					2038:2047	nutritious	2038:2047	not only nutritious but also medicinal	2029:2066	This study shows that functional cereals produced in-vitro extensively are not only nutritious but also medicinal in nature, offering benefits in the management of diabetes and related complications.					
35485965	2	104	theme	different	432:440	arg1	proportion					442:451	different proportion	432:451	different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn	432:617	Functional cereals were produced as ready-to-eat cereals at different proportion with variations in the amount of measured acha flour, soybean flour, and blends of orange peels and moringa leaves while maintaining a constant amount of yellow corn.					
35485965	6	105	theme	vitamin	1642:1648	arg1	C					1650:1650	vitamin C	1642:1650	vitamin C	1642:1650	Commercial breakfast cereals are readily available and consumed irrespective of the fact it has high calorie constituent as more emphasis is placed on their nutrients value which includes vitamins A, vitamin C and calcium for boosting immune system and for proper body growth and functioning.					
35485965	3	106	theme	orange	633:638	arg1	peels					640:644	orange peels	633:644	orange peels	633:644	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
35485965	1	107	theme	starch	286:291	arg1	composition					293:303	starch composition	286:303	starch composition	286:303	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	7	108	theme	health	1909:1914	arg1	patients					1944:1951	diabetic patients	1935:1951	diabetic patients	1935:1951	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	7	108	theme	health	1909:1914	arg1	challenges					1916:1925	health challenges	1909:1925	health challenges such as diabetic patients	1909:1951	However, these commercial breakfast cereals (golden morn) owing to the high maize and millet content present in them are unfavorable to certain consumers or individuals with health challenges such as diabetic patients.					
35485965	1	109	dep	corn	194:197	arg1	leaves					240:245	leaves	240:245	leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro	240:369	This study analyzed the effects of functional cereals processed and produced from yellow corn, soybean, acha, orange peels and moringa leaves' polyphenolic content, glycemic index, starch composition and their effects on α-amylase and α-glucosidase enzymes in vitro.					
35485965	3	110	theme	inhibitory	762:771	arg1	activity					773:780	inhibitory activity	762:780	inhibitory activity	762:780	The produced orange peels and moringa leaves blends inclusion to functional cereals had significantly increased phenolic constituents, showed inhibitory activity on α-amylase and α-glucosidase enzymes in-vitro and reduced glycemic index, starch content and starch to sugar ratios.					
36241315	5	0	theme	acids	837:841	arg1	Levels					809:814	Levels	809:814	Levels of short-chain fatty acids (SCFAs)	809:849	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	1	1	theme	gut	157:159	arg1	consortia					171:179	gut microbial consortia	157:179	gut microbial consortia	157:179	The ecology driving the remodeling of gut microbial consortia with dietary fiber intervention remains incomplete.					
36241315	0	2	theme	temporal	81:88	arg1	response					90:97	the temporal response	77:97	the temporal response in SCFA production	77:116	Dynamics of microbial communities during inulin fermentation associated with the temporal response in SCFA production.					
36241315	6	3	theme	fiber-induced	1152:1164	arg1	changes					1191:1197	fiber-induced microbiome and metabolic changes	1152:1197	fiber-induced microbiome and metabolic changes	1152:1197	This study emphasizes the importance of complex ecology to understand fiber-induced microbiome and metabolic changes, thus providing a reference for predictable dietary responses.					
36241315	2	4	theme	swine	379:383	arg1	breeds					385:390	two swine breeds	375:390	two swine breeds	375:390	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	0	5	theme	SCFA	102:105	arg1	production					107:116	SCFA production	102:116	SCFA production	102:116	Dynamics of microbial communities during inulin fermentation associated with the temporal response in SCFA production.					
36241315	1	6	with	remodeling	143:152	arg1	intervention					200:211	dietary fiber intervention	186:211	dietary fiber intervention	186:211	The ecology driving the remodeling of gut microbial consortia with dietary fiber intervention remains incomplete.					
36241315	2	7	theme	function	309:316	arg1	dynamics					264:271	the short-term dynamics	249:271	the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds	249:390	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	5	8	with	predictable	856:866	arg1	bacteria					881:888	the key bacteria	873:888	the key bacteria	873:888	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	2	9	theme	metabolic	299:307	arg1	function					309:316	metabolic function	299:316	metabolic function	299:316	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	4	10	theme	particular	781:790	arg1	α-diversity					796:806	particular the α-diversity	781:806	particular the α-diversity	781:806	Inulin-induced bacterial succession and individual metabolic change were determined by the original microbial compositions, in particular the α-diversity.					
36241315	4	11	theme	microbial	754:762	arg1	compositions					764:775	the original microbial compositions	741:775	the original microbial compositions	741:775	Inulin-induced bacterial succession and individual metabolic change were determined by the original microbial compositions, in particular the α-diversity.					
36241315	6	12	theme	dietary	1243:1249	arg1	responses					1251:1259	predictable dietary responses	1231:1259	predictable dietary responses	1231:1259	This study emphasizes the importance of complex ecology to understand fiber-induced microbiome and metabolic changes, thus providing a reference for predictable dietary responses.					
36241315	3	13	theme	fermentation	556:567	arg1	stage					569:573	the similar fermentation stage	544:573	the similar fermentation stage	544:573	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	2	14	dep	gut	280:282	arg1	microbiota					284:293	microbiota	284:293	microbiota	284:293	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	1	15	theme	consortia	171:179	arg1	remodeling					143:152	the remodeling	139:152	the remodeling of gut microbial consortia with dietary fiber intervention	139:211	The ecology driving the remodeling of gut microbial consortia with dietary fiber intervention remains incomplete.					
36241315	3	16	theme	gut	440:442	arg1	microbiota					444:453	Different gut microbiota	430:453	Different gut microbiota at a transient temporal time	430:482	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	2	17	from	breeds	385:390	arg1	function					309:316	metabolic function	299:316	metabolic function	299:316	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	2	17	from	breeds	385:390	arg1	dynamics					264:271	the short-term dynamics	249:271	the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds	249:390	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	2	17	from	breeds	385:390	arg1	gut					280:282	the gut microbiota and metabolic function	276:316	gut	280:282	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	5	18	theme	elsdenii	1072:1079	arg1	abundance					959:967	abundance	959:967	abundance	959:967	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	5	18	theme	elsdenii	1072:1079	arg1	interactions					984:995	ecological interactions	973:995	ecological interactions	973:995	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	2	19	theme	fermentation	410:421	arg1	model					423:427	an in vitro fermentation model	398:427	an in vitro fermentation model	398:427	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	1	20	theme	dietary	186:192	arg1	intervention					200:211	dietary fiber intervention	186:211	dietary fiber intervention	186:211	The ecology driving the remodeling of gut microbial consortia with dietary fiber intervention remains incomplete.					
36241315	5	21	theme	delbrueckii	1014:1024	arg1	abundance					959:967	abundance	959:967	abundance	959:967	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	5	21	theme	delbrueckii	1014:1024	arg1	interactions					984:995	ecological interactions	973:995	ecological interactions	973:995	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	2	22	theme	in	401:402	arg1	model					423:427	an in vitro fermentation model	398:427	an in vitro fermentation model	398:427	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	0	23	theme	communities	22:32	arg1	Dynamics					0:7	Dynamics	0:7	Dynamics of microbial communities during inulin fermentation	0:59	Dynamics of microbial communities during inulin fermentation associated with the temporal response in SCFA production.					
36241315	6	24	theme	metabolic	1181:1189	arg1	changes					1191:1197	fiber-induced microbiome and metabolic changes	1152:1197	fiber-induced microbiome and metabolic changes	1152:1197	This study emphasizes the importance of complex ecology to understand fiber-induced microbiome and metabolic changes, thus providing a reference for predictable dietary responses.					
36241315	2	25	theme	inulin	325:330	arg1	fermentation					332:343	inulin fermentation	325:343	inulin fermentation	325:343	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	2	26	from	dynamics	264:271	arg1	breeds					385:390	two swine breeds	375:390	two swine breeds	375:390	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	5	27	theme	thermophilum	1043:1054	arg1	abundance					959:967	abundance	959:967	abundance	959:967	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	5	27	theme	thermophilum	1043:1054	arg1	interactions					984:995	ecological interactions	973:995	ecological interactions	973:995	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	0	28	theme	microbial	12:20	arg1	communities					22:32	microbial communities	12:32	microbial communities	12:32	Dynamics of microbial communities during inulin fermentation associated with the temporal response in SCFA production.					
36241315	0	29	from	response	90:97	arg1	production					107:116	SCFA production	102:116	SCFA production	102:116	Dynamics of microbial communities during inulin fermentation associated with the temporal response in SCFA production.					
36241315	1	30	theme	microbial	161:169	arg1	consortia					171:179	gut microbial consortia	157:179	gut microbial consortia	157:179	The ecology driving the remodeling of gut microbial consortia with dietary fiber intervention remains incomplete.					
36241315	3	31	theme	similar	496:502	arg1	response					504:511	a similar response	494:511	a similar response to inulin intervention such as the similar fermentation stage	494:573	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	0	32	theme	inulin	41:46	arg1	fermentation					48:59	inulin fermentation	41:59	inulin fermentation	41:59	Dynamics of microbial communities during inulin fermentation associated with the temporal response in SCFA production.					
36241315	6	33	theme	predictable	1231:1241	arg1	responses					1251:1259	predictable dietary responses	1231:1259	predictable dietary responses	1231:1259	This study emphasizes the importance of complex ecology to understand fiber-induced microbiome and metabolic changes, thus providing a reference for predictable dietary responses.					
36241315	5	34	theme	regression	897:906	arg1	model					908:912	the regression model	893:912	the regression model	893:912	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	2	35	theme	short-term	253:262	arg1	dynamics					264:271	the short-term dynamics	249:271	the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds	249:390	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	1	36	theme	fiber	194:198	arg1	intervention					200:211	dietary fiber intervention	186:211	dietary fiber intervention	186:211	The ecology driving the remodeling of gut microbial consortia with dietary fiber intervention remains incomplete.					
36241315	4	37	theme	metabolic	705:713	arg1	change					715:720	individual metabolic change	694:720	individual metabolic change	694:720	Inulin-induced bacterial succession and individual metabolic change were determined by the original microbial compositions, in particular the α-diversity.					
36241315	3	38	theme	transient	460:468	arg1	time					479:482	a transient temporal time	458:482	a transient temporal time	458:482	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	3	39	theme	microbial	633:641	arg1	diversity					643:651	microbial diversity	633:651	microbial diversity	633:651	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	3	40	from	time	479:482	arg1	microbiota					444:453	Different gut microbiota	430:453	Different gut microbiota at a transient temporal time	430:482	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	4	41	theme	individual	694:703	arg1	change					715:720	individual metabolic change	694:720	individual metabolic change	694:720	Inulin-induced bacterial succession and individual metabolic change were determined by the original microbial compositions, in particular the α-diversity.					
36241315	3	42	theme	gradual	608:614	arg1	stabilization					616:628	gradual stabilization	608:628	gradual stabilization of microbial diversity	608:651	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	5	43	dep	abundance	959:967	arg1	the					955:957	the	955:957	the	955:957	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	3	44	theme	inulin	516:521	arg1	intervention					523:534	inulin intervention	516:534	inulin intervention	516:534	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	3	45	theme	Different	430:438	arg1	microbiota					444:453	Different gut microbiota	430:453	Different gut microbiota at a transient temporal time	430:482	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	2	46	with	gut	280:282	arg1	microbiota					359:368	distinct microbiota	350:368	distinct microbiota	350:368	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	6	47	theme	microbiome	1166:1175	arg1	changes					1191:1197	fiber-induced microbiome and metabolic changes	1152:1197	fiber-induced microbiome and metabolic changes	1152:1197	This study emphasizes the importance of complex ecology to understand fiber-induced microbiome and metabolic changes, thus providing a reference for predictable dietary responses.					
36241315	5	48	theme	key	877:879	arg1	bacteria					881:888	the key bacteria	873:888	the key bacteria	873:888	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	5	49	theme	ecological	973:982	arg1	interactions					984:995	ecological interactions	973:995	ecological interactions	973:995	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	2	50	theme	gut	280:282	arg1	dynamics					264:271	the short-term dynamics	249:271	the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds	249:390	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	3	51	theme	rapid	581:585	arg1	response					587:594	a rapid response	579:594	a rapid response followed by gradual stabilization of microbial diversity	579:651	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	6	52	theme	complex	1122:1128	arg1	ecology					1130:1136	complex ecology	1122:1136	complex ecology	1122:1136	This study emphasizes the importance of complex ecology to understand fiber-induced microbiome and metabolic changes, thus providing a reference for predictable dietary responses.					
36241315	2	53	dep	in	401:402	arg1	vitro					404:408	vitro	404:408	vitro	404:408	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	2	54	theme	distinct	350:357	arg1	microbiota					359:368	distinct microbiota	350:368	distinct microbiota	350:368	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	5	55	theme	short-chain	819:829	arg1	SCFAs					844:848	SCFAs	844:848	SCFAs	844:848	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	5	55	theme	short-chain	819:829	arg1	acids					837:841	short-chain fatty acids	819:841	short-chain fatty acids (SCFAs)	819:849	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	6	56	theme	ecology	1130:1136	arg1	importance					1108:1117	the importance	1104:1117	the importance of complex ecology	1104:1136	This study emphasizes the importance of complex ecology to understand fiber-induced microbiome and metabolic changes, thus providing a reference for predictable dietary responses.					
36241315	3	57	theme	diversity	643:651	arg1	stabilization					616:628	gradual stabilization	608:628	gradual stabilization of microbial diversity	608:651	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	4	58	theme	bacterial	669:677	arg1	succession					679:688	Inulin-induced bacterial succession	654:688	Inulin-induced bacterial succession	654:688	Inulin-induced bacterial succession and individual metabolic change were determined by the original microbial compositions, in particular the α-diversity.					
36241315	4	59	theme	original	745:752	arg1	compositions					764:775	the original microbial compositions	741:775	the original microbial compositions	741:775	Inulin-induced bacterial succession and individual metabolic change were determined by the original microbial compositions, in particular the α-diversity.					
36241315	3	60	theme	temporal	470:477	arg1	time					479:482	a transient temporal time	458:482	a transient temporal time	458:482	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	2	61	with	function	309:316	arg1	microbiota					359:368	distinct microbiota	350:368	distinct microbiota	350:368	We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model.					
36241315	5	62	theme	fatty	831:835	arg1	SCFAs					844:848	SCFAs	844:848	SCFAs	844:848	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	5	62	theme	fatty	831:835	arg1	acids					837:841	short-chain fatty acids	819:841	short-chain fatty acids (SCFAs)	819:849	Levels of short-chain fatty acids (SCFAs) were predictable with the key bacteria by the regression model, especially butyrate was associated with the abundance and ecological interactions of Lactobacillus delbrueckii, Bifidobacterium thermophilum and Megasphaera elsdenii.					
36241315	3	63	theme	similar	548:554	arg1	stage					569:573	the similar fermentation stage	544:573	the similar fermentation stage	544:573	Different gut microbiota at a transient temporal time displayed a similar response to inulin intervention such as the similar fermentation stage and a rapid response followed by gradual stabilization of microbial diversity.					
36241315	4	64	theme	Inulin-induced	654:667	arg1	succession					679:688	Inulin-induced bacterial succession	654:688	Inulin-induced bacterial succession	654:688	Inulin-induced bacterial succession and individual metabolic change were determined by the original microbial compositions, in particular the α-diversity.					
37367215	9	0	theme	significant	1112:1122	arg1	difference					1124:1133	no significant difference	1109:1133	no significant difference in lactose, protein, bacteria, or IgA content	1109:1179	There was no significant difference in lactose, protein, bacteria, or IgA content over storage duration (p = 0.649, p = 0.690, and p = 0.385, p = 0.805, respectively).					
37367215	6	1	theme	Aerobic	854:860	arg1	bacteria					862:869	Aerobic bacteria	854:869	Aerobic bacteria	854:869	Aerobic bacteria, protein, lactose, and immunoglobulin A (IgA) content were measured.					
37367215	1	2	from	milk	279:282	arg1	States					304:309	the United States	293:309	the United States	293:309	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	10	3	theme	aerobic	1273:1279	arg1	bacteria					1281:1288	Total aerobic bacteria	1267:1288	Total aerobic bacteria	1267:1288	Total aerobic bacteria were less than 102 colony-forming units (CFUs) in 81% of the time points tested for DHM samples.					
37367215	1	4	theme	Milk	174:177	arg1	Association					187:197	The Human Milk Banking Association	164:197	The Human Milk Banking Association of North America (HMBANA)	164:223	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	7	5	theme	variance	1034:1041	arg1	analysis					1022:1029	repeated measures analysis	1004:1029	repeated measures analysis of variance and mixed models test	1004:1063	Longitudinal changes between 0 and 96 hours were analyzed using repeated measures analysis of variance and mixed models test.					
37367215	3	6	theme	Twenty-five	552:562	arg1	samples					571:577	Twenty-five unique samples	552:577	Twenty-five unique samples of DHM with limited bacterial growth postpasteurization	552:633	Twenty-five unique samples of DHM with limited bacterial growth postpasteurization were collected from two HMBANA milk banks.					
37367215	11	7	from	points	1486:1491	arg1	many					1419:1422	many	1419:1422	many	1419:1422	Total aerobic bacteria were too many to count (>300 CFUs) in the infant formula sample at all time points.					
37367215	7	8	dep	variance	1034:1041	arg1	test					1060:1063	test	1060:1063	test	1060:1063	Longitudinal changes between 0 and 96 hours were analyzed using repeated measures analysis of variance and mixed models test.					
37367215	7	9	theme	repeated	1004:1011	arg1	analysis					1022:1029	repeated measures analysis	1004:1029	repeated measures analysis of variance and mixed models test	1004:1063	Longitudinal changes between 0 and 96 hours were analyzed using repeated measures analysis of variance and mixed models test.					
37367215	1	10	theme	Holder	357:362	arg1	pasteurization					364:377	Holder pasteurization	357:377	Holder pasteurization	357:377	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	3	11	theme	DHM	582:584	arg1	samples					571:577	Twenty-five unique samples	552:577	Twenty-five unique samples of DHM with limited bacterial growth postpasteurization	552:633	Twenty-five unique samples of DHM with limited bacterial growth postpasteurization were collected from two HMBANA milk banks.					
37367215	11	12	from	sample	1467:1472	arg1	many					1419:1422	many	1419:1422	many	1419:1422	Total aerobic bacteria were too many to count (>300 CFUs) in the infant formula sample at all time points.					
37367215	6	13	theme	immunoglobulin	894:907	arg1	IgA					912:914	IgA	912:914	IgA	912:914	Aerobic bacteria, protein, lactose, and immunoglobulin A (IgA) content were measured.					
37367215	6	13	theme	immunoglobulin	894:907	arg1	A					909:909	immunoglobulin A	894:909	immunoglobulin A (IgA) content	894:923	Aerobic bacteria, protein, lactose, and immunoglobulin A (IgA) content were measured.					
37367215	0	14	theme	Bacterial	146:154	arg1	Growth					156:161	Bacterial Growth	146:161	Bacterial Growth	146:161	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	9	15	theme	storage	1186:1192	arg1	duration					1194:1201	storage duration	1186:1201	storage duration (p = 0.649, p = 0.690, and p = 0.385, p = 0.805, respectively)	1186:1264	There was no significant difference in lactose, protein, bacteria, or IgA content over storage duration (p = 0.649, p = 0.690, and p = 0.385, p = 0.805, respectively).					
37367215	12	16	theme	growth	1551:1556	arg1	postpasteurization					1558:1575	low bacteria growth postpasteurization	1538:1575	low bacteria growth postpasteurization	1538:1575	In periods of high demand for DHM, DHM with low bacteria growth postpasteurization may be an option as a supplemental food for the growing number of healthy infants who receive DHM.					
37367215	2	17	theme	storage	543:549	arg1	days					522:525	4 days	520:525	4 days of refrigerated storage	520:549	This study aimed to determine if nutrient and bacterial composition of DHM with limited bacterial presence after pasteurization change over 4 days of refrigerated storage.					
37367215	11	18	theme	Total	1387:1391	arg1	bacteria					1401:1408	Total aerobic bacteria	1387:1408	Total aerobic bacteria	1387:1408	Total aerobic bacteria were too many to count (>300 CFUs) in the infant formula sample at all time points.					
37367215	3	19	theme	growth	609:614	arg1	postpasteurization					616:633	limited bacterial growth postpasteurization	591:633	limited bacterial growth postpasteurization	591:633	Twenty-five unique samples of DHM with limited bacterial growth postpasteurization were collected from two HMBANA milk banks.					
37367215	2	20	theme	bacterial	468:476	arg1	presence					478:485	limited bacterial presence	460:485	limited bacterial presence	460:485	This study aimed to determine if nutrient and bacterial composition of DHM with limited bacterial presence after pasteurization change over 4 days of refrigerated storage.					
37367215	4	21	theme	Infant	678:683	arg1	formula					685:691	Infant formula	678:691	Infant formula	678:691	Infant formula was used as a comparison.					
37367215	4	21	theme	Infant	678:683	arg1	comparison					707:716	a comparison	705:716	a comparison	705:716	Infant formula was used as a comparison.					
37367215	0	22	theme	Milk	51:54	arg1	Storage					28:34	Refrigerated Storage	15:34	Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization	15:114	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	3	23	theme	limited	591:597	arg1	postpasteurization					616:633	limited bacterial growth postpasteurization	591:633	limited bacterial growth postpasteurization	591:633	Twenty-five unique samples of DHM with limited bacterial growth postpasteurization were collected from two HMBANA milk banks.					
37367215	9	24	from	difference	1124:1133	arg1	lactose					1138:1144	lactose	1138:1144	lactose	1138:1144	There was no significant difference in lactose, protein, bacteria, or IgA content over storage duration (p = 0.649, p = 0.690, and p = 0.385, p = 0.805, respectively).					
37367215	9	24	from	difference	1124:1133	arg1	content					1173:1179	IgA content	1169:1179	IgA content	1169:1179	There was no significant difference in lactose, protein, bacteria, or IgA content over storage duration (p = 0.649, p = 0.690, and p = 0.385, p = 0.805, respectively).					
37367215	9	24	from	difference	1124:1133	arg1	bacteria					1156:1163	bacteria	1156:1163	bacteria	1156:1163	There was no significant difference in lactose, protein, bacteria, or IgA content over storage duration (p = 0.649, p = 0.690, and p = 0.385, p = 0.805, respectively).					
37367215	9	24	from	difference	1124:1133	arg1	protein					1147:1153	protein	1147:1153	protein	1147:1153	There was no significant difference in lactose, protein, bacteria, or IgA content over storage duration (p = 0.649, p = 0.690, and p = 0.385, p = 0.805, respectively).					
37367215	0	25	theme	Limited	61:67	arg1	Presence					79:86	Limited Bacterial Presence	61:86	Limited Bacterial Presence	61:86	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	12	26	theme	low	1538:1540	arg1	postpasteurization					1558:1575	low bacteria growth postpasteurization	1538:1575	low bacteria growth postpasteurization	1538:1575	In periods of high demand for DHM, DHM with low bacteria growth postpasteurization may be an option as a supplemental food for the growing number of healthy infants who receive DHM.					
37367215	3	27	with	samples	571:577	arg1	postpasteurization					616:633	limited bacterial growth postpasteurization	591:633	limited bacterial growth postpasteurization	591:633	Twenty-five unique samples of DHM with limited bacterial growth postpasteurization were collected from two HMBANA milk banks.					
37367215	4	28	used	used	697:700	arg2	comparison					707:716	a comparison	705:716	a comparison	705:716	Infant formula was used as a comparison.					
37367215	4	28	used	used	697:700	arg2	formula					685:691	Infant formula	678:691	Infant formula	678:691	Infant formula was used as a comparison.					
37367215	12	29	theme	demand	1513:1518	arg1	periods					1497:1503	periods	1497:1503	periods of high demand for DHM	1497:1526	In periods of high demand for DHM, DHM with low bacteria growth postpasteurization may be an option as a supplemental food for the growing number of healthy infants who receive DHM.					
37367215	0	30	theme	Holder	94:99	arg1	Pasteurization					101:114	Holder Pasteurization	94:114	Holder Pasteurization	94:114	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	2	31	theme	nutrient	413:420	arg1	composition					436:446	nutrient and bacterial composition	413:446	nutrient and bacterial composition of DHM with limited bacterial presence after pasteurization	413:506	This study aimed to determine if nutrient and bacterial composition of DHM with limited bacterial presence after pasteurization change over 4 days of refrigerated storage.					
37367215	10	32	theme	points	1356:1361	arg1	points					1356:1361	the time points	1347:1361	the time points tested for DHM samples	1347:1384	Total aerobic bacteria were less than 102 colony-forming units (CFUs) in 81% of the time points tested for DHM samples.					
37367215	10	32	theme	points	1356:1361	arg1	%					1342:1342	81%	1340:1342	81% of the time points tested for DHM samples	1340:1384	Total aerobic bacteria were less than 102 colony-forming units (CFUs) in 81% of the time points tested for DHM samples.					
37367215	13	33	theme	bacterial	1714:1722	arg1	strains					1724:1730	the bacterial strains	1710:1730	the bacterial strains	1710:1730	Future studies should investigate the bacterial strains in this milk.					
37367215	5	34	theme	24-hour	796:802	arg1	intervals					804:812	24-hour intervals	796:812	24-hour intervals beginning at hour 0 to 96 for analysis	796:851	Samples were stored in the refrigerator and a portion of milk was removed at 24-hour intervals beginning at hour 0 to 96 for analysis.					
37367215	1	35	theme	human	273:277	arg1	DHM					285:287	DHM	285:287	DHM	285:287	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	1	35	theme	human	273:277	arg1	milk					279:282	donor human milk	267:282	donor human milk (DHM)	267:288	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	12	36	from	option	1587:1592	arg1	periods					1497:1503	periods	1497:1503	periods of high demand for DHM	1497:1526	In periods of high demand for DHM, DHM with low bacteria growth postpasteurization may be an option as a supplemental food for the growing number of healthy infants who receive DHM.					
37367215	0	37	theme	Storage	28:34	arg1	Effects					4:10	The Effects	0:10	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.	0:162	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	12	38	theme	infants	1651:1657	arg1	number					1633:1638	the growing number	1621:1638	the growing number of healthy infants who receive DHM	1621:1673	In periods of high demand for DHM, DHM with low bacteria growth postpasteurization may be an option as a supplemental food for the growing number of healthy infants who receive DHM.					
37367215	2	39	theme	bacterial	426:434	arg1	composition					436:446	nutrient and bacterial composition	413:446	nutrient and bacterial composition of DHM with limited bacterial presence after pasteurization	413:506	This study aimed to determine if nutrient and bacterial composition of DHM with limited bacterial presence after pasteurization change over 4 days of refrigerated storage.					
37367215	5	40	theme	milk	776:779	arg1	portion					765:771	a portion	763:771	a portion of milk	763:779	Samples were stored in the refrigerator and a portion of milk was removed at 24-hour intervals beginning at hour 0 to 96 for analysis.					
37367215	7	41	theme	mixed	1047:1051	arg1	models					1053:1058	mixed models	1047:1058	mixed models	1047:1058	Longitudinal changes between 0 and 96 hours were analyzed using repeated measures analysis of variance and mixed models test.					
37367215	11	42	theme	infant	1452:1457	arg1	sample					1467:1472	the infant formula sample	1448:1472	the infant formula sample	1448:1472	Total aerobic bacteria were too many to count (>300 CFUs) in the infant formula sample at all time points.					
37367215	11	43	theme	formula	1459:1465	arg1	sample					1467:1472	the infant formula sample	1448:1472	the infant formula sample	1448:1472	Total aerobic bacteria were too many to count (>300 CFUs) in the infant formula sample at all time points.					
37367215	12	44	theme	growing	1625:1631	arg1	number					1633:1638	the growing number	1621:1638	the growing number of healthy infants who receive DHM	1621:1673	In periods of high demand for DHM, DHM with low bacteria growth postpasteurization may be an option as a supplemental food for the growing number of healthy infants who receive DHM.					
37367215	3	45	theme	HMBANA	659:664	arg1	banks					671:675	two HMBANA milk banks	655:675	two HMBANA milk banks	655:675	Twenty-five unique samples of DHM with limited bacterial growth postpasteurization were collected from two HMBANA milk banks.					
37367215	0	46	with	Storage	28:34	arg1	Presence					79:86	Limited Bacterial Presence	61:86	Limited Bacterial Presence	61:86	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	1	47	theme	purity	234:239	arg1	standards					253:261	the purity and quality standards	230:261	the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization	230:377	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	12	48	theme	supplemental	1599:1610	arg1	food					1612:1615	a supplemental food	1597:1615	a supplemental food for the growing number of healthy infants who receive DHM	1597:1673	In periods of high demand for DHM, DHM with low bacteria growth postpasteurization may be an option as a supplemental food for the growing number of healthy infants who receive DHM.					
37367215	7	49	theme	models	1053:1058	arg1	analysis					1022:1029	repeated measures analysis	1004:1029	repeated measures analysis of variance and mixed models test	1004:1063	Longitudinal changes between 0 and 96 hours were analyzed using repeated measures analysis of variance and mixed models test.					
37367215	10	50	theme	colony-forming	1309:1322	arg1	CFUs					1331:1334	CFUs	1331:1334	CFUs	1331:1334	Total aerobic bacteria were less than 102 colony-forming units (CFUs) in 81% of the time points tested for DHM samples.					
37367215	10	50	theme	colony-forming	1309:1322	arg1	units					1324:1328	102 colony-forming units	1305:1328	102 colony-forming units (CFUs) in 81% of the time points tested for DHM samples	1305:1384	Total aerobic bacteria were less than 102 colony-forming units (CFUs) in 81% of the time points tested for DHM samples.					
37367215	1	51	theme	quality	245:251	arg1	standards					253:261	the purity and quality standards	230:261	the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization	230:377	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	9	52	dep	duration	1194:1201	arg1	p = 0.805					1241:1249	p = 0.805	1241:1249	p = 0.805	1241:1249	There was no significant difference in lactose, protein, bacteria, or IgA content over storage duration (p = 0.649, p = 0.690, and p = 0.385, p = 0.805, respectively).					
37367215	9	52	dep	duration	1194:1201	arg1	p = 0.690					1215:1223	p = 0.690	1215:1223	p = 0.690	1215:1223	There was no significant difference in lactose, protein, bacteria, or IgA content over storage duration (p = 0.649, p = 0.690, and p = 0.385, p = 0.805, respectively).					
37367215	9	52	dep	duration	1194:1201	arg1	p = 0.385					1230:1238	p = 0.385	1230:1238	p = 0.385	1230:1238	There was no significant difference in lactose, protein, bacteria, or IgA content over storage duration (p = 0.649, p = 0.690, and p = 0.385, p = 0.805, respectively).					
37367215	9	52	dep	duration	1194:1201	arg1	p = 0.649					1204:1212	p = 0.649	1204:1212	p = 0.649	1204:1212	There was no significant difference in lactose, protein, bacteria, or IgA content over storage duration (p = 0.649, p = 0.690, and p = 0.385, p = 0.805, respectively).					
37367215	1	53	theme	Human	168:172	arg1	Association					187:197	The Human Milk Banking Association	164:197	The Human Milk Banking Association of North America (HMBANA)	164:223	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	0	54	theme	Donor	39:43	arg1	Milk					51:54	Donor Human Milk	39:54	Donor Human Milk	39:54	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	1	55	theme	Banking	179:185	arg1	Association					187:197	The Human Milk Banking Association	164:197	The Human Milk Banking Association of North America (HMBANA)	164:223	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	7	56	theme	measures	1013:1020	arg1	analysis					1022:1029	repeated measures analysis	1004:1029	repeated measures analysis of variance and mixed models test	1004:1063	Longitudinal changes between 0 and 96 hours were analyzed using repeated measures analysis of variance and mixed models test.					
37367215	9	57	theme	IgA	1169:1171	arg1	content					1173:1179	IgA content	1169:1179	IgA content	1169:1179	There was no significant difference in lactose, protein, bacteria, or IgA content over storage duration (p = 0.649, p = 0.690, and p = 0.385, p = 0.805, respectively).					
37367215	1	58	theme	America	208:214	arg1	Association					187:197	The Human Milk Banking Association	164:197	The Human Milk Banking Association of North America (HMBANA)	164:223	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	6	59	theme	A	909:909	arg1	content					917:923	immunoglobulin A (IgA) content	894:923	immunoglobulin A (IgA) content	894:923	Aerobic bacteria, protein, lactose, and immunoglobulin A (IgA) content were measured.					
37367215	3	60	theme	unique	564:569	arg1	samples					571:577	Twenty-five unique samples	552:577	Twenty-five unique samples of DHM with limited bacterial growth postpasteurization	552:633	Twenty-five unique samples of DHM with limited bacterial growth postpasteurization were collected from two HMBANA milk banks.					
37367215	0	61	theme	Nutrient	119:126	arg1	Concentration					128:140	Nutrient Concentration	119:140	Nutrient Concentration	119:140	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	3	62	theme	bacterial	599:607	arg1	postpasteurization					616:633	limited bacterial growth postpasteurization	591:633	limited bacterial growth postpasteurization	591:633	Twenty-five unique samples of DHM with limited bacterial growth postpasteurization were collected from two HMBANA milk banks.					
37367215	12	63	theme	bacteria	1542:1549	arg1	postpasteurization					1558:1575	low bacteria growth postpasteurization	1538:1575	low bacteria growth postpasteurization	1538:1575	In periods of high demand for DHM, DHM with low bacteria growth postpasteurization may be an option as a supplemental food for the growing number of healthy infants who receive DHM.					
37367215	2	64	theme	refrigerated	530:541	arg1	storage					543:549	refrigerated storage	530:549	refrigerated storage	530:549	This study aimed to determine if nutrient and bacterial composition of DHM with limited bacterial presence after pasteurization change over 4 days of refrigerated storage.					
37367215	7	65	theme	Longitudinal	940:951	arg1	changes					953:959	Longitudinal changes	940:959	Longitudinal changes between 0 and 96 hours	940:982	Longitudinal changes between 0 and 96 hours were analyzed using repeated measures analysis of variance and mixed models test.					
37367215	11	66	from	many	1419:1422	arg1	sample					1467:1472	the infant formula sample	1448:1472	the infant formula sample	1448:1472	Total aerobic bacteria were too many to count (>300 CFUs) in the infant formula sample at all time points.					
37367215	2	67	theme	limited	460:466	arg1	presence					478:485	limited bacterial presence	460:485	limited bacterial presence	460:485	This study aimed to determine if nutrient and bacterial composition of DHM with limited bacterial presence after pasteurization change over 4 days of refrigerated storage.					
37367215	0	68	theme	Bacterial	69:77	arg1	Presence					79:86	Limited Bacterial Presence	61:86	Limited Bacterial Presence	61:86	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	1	69	theme	United	297:302	arg1	States					304:309	the United States	293:309	the United States	293:309	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	2	70	theme	DHM	451:453	arg1	composition					436:446	nutrient and bacterial composition	413:446	nutrient and bacterial composition of DHM with limited bacterial presence after pasteurization	413:506	This study aimed to determine if nutrient and bacterial composition of DHM with limited bacterial presence after pasteurization change over 4 days of refrigerated storage.					
37367215	0	71	from	Effects	4:10	arg1	Concentration					128:140	Nutrient Concentration	119:140	Nutrient Concentration	119:140	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	0	71	from	Effects	4:10	arg1	Growth					156:161	Bacterial Growth	146:161	Bacterial Growth	146:161	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	11	72	theme	aerobic	1393:1399	arg1	bacteria					1401:1408	Total aerobic bacteria	1387:1408	Total aerobic bacteria	1387:1408	Total aerobic bacteria were too many to count (>300 CFUs) in the infant formula sample at all time points.					
37367215	2	73	with	DHM	451:453	arg1	presence					478:485	limited bacterial presence	460:485	limited bacterial presence	460:485	This study aimed to determine if nutrient and bacterial composition of DHM with limited bacterial presence after pasteurization change over 4 days of refrigerated storage.					
37367215	12	74	theme	high	1508:1511	arg1	demand					1513:1518	high demand	1508:1518	high demand for DHM	1508:1526	In periods of high demand for DHM, DHM with low bacteria growth postpasteurization may be an option as a supplemental food for the growing number of healthy infants who receive DHM.					
37367215	1	75	theme	bacterial	332:340	arg1	presence					342:349	zero bacterial presence	327:349	zero bacterial presence	327:349	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	10	76	theme	time	1351:1354	arg1	points					1356:1361	the time points	1347:1361	the time points tested for DHM samples	1347:1384	Total aerobic bacteria were less than 102 colony-forming units (CFUs) in 81% of the time points tested for DHM samples.					
37367215	0	77	theme	Refrigerated	15:26	arg1	Storage					28:34	Refrigerated Storage	15:34	Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization	15:114	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	1	78	theme	donor	267:271	arg1	DHM					285:287	DHM	285:287	DHM	285:287	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	1	78	theme	donor	267:271	arg1	milk					279:282	donor human milk	267:282	donor human milk (DHM)	267:288	The Human Milk Banking Association of North America (HMBANA) sets the purity and quality standards for donor human milk (DHM) in the United States, which includes zero bacterial presence after Holder pasteurization.					
37367215	12	79	with	DHM	1529:1531	arg1	postpasteurization					1558:1575	low bacteria growth postpasteurization	1538:1575	low bacteria growth postpasteurization	1538:1575	In periods of high demand for DHM, DHM with low bacteria growth postpasteurization may be an option as a supplemental food for the growing number of healthy infants who receive DHM.					
37367215	13	80	theme	Future	1676:1681	arg1	studies					1683:1689	Future studies	1676:1689	Future studies	1676:1689	Future studies should investigate the bacterial strains in this milk.					
37367215	10	81	theme	DHM	1374:1376	arg1	samples					1378:1384	DHM samples	1374:1384	DHM samples	1374:1384	Total aerobic bacteria were less than 102 colony-forming units (CFUs) in 81% of the time points tested for DHM samples.					
37367215	10	82	theme	Total	1267:1271	arg1	bacteria					1281:1288	Total aerobic bacteria	1267:1288	Total aerobic bacteria	1267:1288	Total aerobic bacteria were less than 102 colony-forming units (CFUs) in 81% of the time points tested for DHM samples.					
37367215	0	83	theme	Human	45:49	arg1	Milk					51:54	Donor Human Milk	39:54	Donor Human Milk	39:54	The Effects of Refrigerated Storage of Donor Human Milk with Limited Bacterial Presence After Holder Pasteurization on Nutrient Concentration and Bacterial Growth.					
37367215	12	84	theme	healthy	1643:1649	arg1	infants					1651:1657	healthy infants	1643:1657	healthy infants who receive DHM	1643:1673	In periods of high demand for DHM, DHM with low bacteria growth postpasteurization may be an option as a supplemental food for the growing number of healthy infants who receive DHM.					
37367215	3	85	theme	milk	666:669	arg1	banks					671:675	two HMBANA milk banks	655:675	two HMBANA milk banks	655:675	Twenty-five unique samples of DHM with limited bacterial growth postpasteurization were collected from two HMBANA milk banks.					
37367215	10	86	from	units	1324:1328	arg1	points					1356:1361	the time points	1347:1361	the time points tested for DHM samples	1347:1384	Total aerobic bacteria were less than 102 colony-forming units (CFUs) in 81% of the time points tested for DHM samples.					
37367215	10	86	from	units	1324:1328	arg1	%					1342:1342	81%	1340:1342	81% of the time points tested for DHM samples	1340:1384	Total aerobic bacteria were less than 102 colony-forming units (CFUs) in 81% of the time points tested for DHM samples.					
37367215	5	87	dep	hour	827:830	arg1	to					834:835	to	834:835	to	834:835	Samples were stored in the refrigerator and a portion of milk was removed at 24-hour intervals beginning at hour 0 to 96 for analysis.					
37367215	11	88	theme	time	1481:1484	arg1	points					1486:1491	all time points	1477:1491	all time points	1477:1491	Total aerobic bacteria were too many to count (>300 CFUs) in the infant formula sample at all time points.					
35699986	4	0	with	strains	280:286	arg1	motion					301:306	gliding motion	293:306	gliding motion (MYSH2T, MJ1aT and dk17T)	293:332	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	11	1	theme	19906T=NBRC	1607:1617	arg1	113667T					1619:1625	=KACC 19906T=NBRC 113667T	1601:1625	=KACC 19906T=NBRC 113667T	1601:1625	nov. (=KACC 19906T=NBRC 113667T) and Mucilaginibacter pallidiroseus sp.					
35699986	11	1	theme	19906T=NBRC	1607:1617	arg1	nov					1595:1597	nov	1595:1597	nov	1595:1597	nov. (=KACC 19906T=NBRC 113667T) and Mucilaginibacter pallidiroseus sp.					
35699986	7	2	theme	CAZys	841:845	arg1	gene					854:857	the CAZys coding gene	837:857	the CAZys coding gene of KAAS	837:865	According to the CAZys coding gene of KAAS, MYSH2T and MJ1aT were interpreted as strains containing both GH18 and 19 family coding genes, except for dk17T, which shows only GH19 family genes.					
35699986	5	3	theme	16S	425:427	arg1	sequences					439:447	the 16S rRNA gene sequences	421:447	the 16S rRNA gene sequences	421:447	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	5	4	theme	rRNA	429:432	arg1	sequences					439:447	the 16S rRNA gene sequences	421:447	the 16S rRNA gene sequences	421:447	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	6	5	dep	feature	718:724	arg1	ω6					752:753	ω6	752:753	ω6	752:753	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	5	dep	feature	718:724	arg1	ω7c					735:737	ω7c	735:737	ω7c	735:737	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	13	6	theme	emended	1717:1723	arg1	description					1725:1735	An emended description	1714:1735	An emended description of the genus Mucilaginibacter	1714:1765	An emended description of the genus Mucilaginibacter is proposed.					
35699986	12	7	theme	=KACC	1673:1677	arg1	113666T					1691:1697	=KACC 19907T=NBRC 113666T	1673:1697	=KACC 19907T=NBRC 113666T	1673:1697	nov. (=KACC 19907T=NBRC 113666T) are proposed.					
35699986	12	7	theme	=KACC	1673:1677	arg1	nov					1667:1669	nov	1667:1669	nov	1667:1669	nov. (=KACC 19907T=NBRC 113666T) are proposed.					
35699986	5	8	theme	high	605:608	arg1	similarity					628:637	a high 16S rRNA sequence similarity	603:637	a high 16S rRNA sequence similarity	603:637	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	1	9	theme	Mucilaginibacter	56:71	arg1	sp					87:88	Mucilaginibacter achroorhodeus sp	56:88	Mucilaginibacter achroorhodeus sp	56:88	nov., Mucilaginibacter achroorhodeus sp.					
35699986	1	9	theme	Mucilaginibacter	56:71	arg1	nov.					50:53	nov.	50:53	nov.	50:53	nov., Mucilaginibacter achroorhodeus sp.					
35699986	5	10	theme	gene	434:437	arg1	sequences					439:447	the 16S rRNA gene sequences	421:447	the 16S rRNA gene sequences	421:447	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	9	11	theme	distinct	1240:1247	arg1	differences					1311:1321	distinct morphological, physiological, chemotaxonomic and phylogenetic differences	1240:1321	distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours	1240:1364	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	5	12	theme	novel	545:549	arg1	species					551:557	novel species	545:557	novel species of the genus Mucilaginibacter	545:587	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	3	13	theme	genus	171:175	arg1	Mucilaginibacter					177:192	the genus Mucilaginibacter	167:192	the genus Mucilaginibacter	167:192	nov. and emended description of the genus Mucilaginibacter.					
35699986	1	14	theme	achroorhodeus	73:85	arg1	sp					87:88	Mucilaginibacter achroorhodeus sp	56:88	Mucilaginibacter achroorhodeus sp	56:88	nov., Mucilaginibacter achroorhodeus sp.					
35699986	1	14	theme	achroorhodeus	73:85	arg1	nov.					50:53	nov.	50:53	nov.	50:53	nov., Mucilaginibacter achroorhodeus sp.					
35699986	6	15	theme	novel	687:691	arg1	strains					693:699	These novel strains	681:699	These novel strains	681:699	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	5	16	dep	%	660:660	arg1	i.e.					640:643	i.e.	640:643	i.e.	640:643	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	9	17	theme	morphological	1249:1261	arg1	differences					1311:1321	distinct morphological, physiological, chemotaxonomic and phylogenetic differences	1240:1321	distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours	1240:1364	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	5	18	theme	rRNA	614:617	arg1	similarity					628:637	a high 16S rRNA sequence similarity	603:637	a high 16S rRNA sequence similarity	603:637	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	5	19	theme	16S	610:612	arg1	similarity					628:637	a high 16S rRNA sequence similarity	603:637	a high 16S rRNA sequence similarity	603:637	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	9	20	theme	conchicola	1513:1522	arg1	sp					1524:1525	conchicola sp	1513:1525	conchicola sp	1513:1525	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	5	21	dep	exhibited	593:601	arg1	%					660:660	more than 97.2 %	645:660	more than 97.2 %	645:660	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	4	22	attach	isolated	339:346	arg2	strains					280:286	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains	195:286	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T)	195:332	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	4	22	attach	isolated	339:346	arg1	seashells					353:361	seashells	353:361	seashells	353:361	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	4	22	attach	isolated	339:346	arg1	foxtail					373:379	foxtail	373:379	foxtail	373:379	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	4	22	attach	isolated	339:346	arg1	soil					364:367	soil	364:367	soil	364:367	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	11	23	theme	=KACC	1601:1605	arg1	113667T					1619:1625	=KACC 19906T=NBRC 113667T	1601:1625	=KACC 19906T=NBRC 113667T	1601:1625	nov. (=KACC 19906T=NBRC 113667T) and Mucilaginibacter pallidiroseus sp.					
35699986	11	23	theme	=KACC	1601:1605	arg1	nov					1595:1597	nov	1595:1597	nov	1595:1597	nov. (=KACC 19906T=NBRC 113667T) and Mucilaginibacter pallidiroseus sp.					
35699986	5	24	theme	sequence	619:626	arg1	similarity					628:637	a high 16S rRNA sequence similarity	603:637	a high 16S rRNA sequence similarity	603:637	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	12	25	theme	19907T=NBRC	1679:1689	arg1	113666T					1691:1697	=KACC 19907T=NBRC 113666T	1673:1697	=KACC 19907T=NBRC 113666T	1673:1697	nov. (=KACC 19907T=NBRC 113666T) are proposed.					
35699986	12	25	theme	19907T=NBRC	1679:1689	arg1	nov					1667:1669	nov	1667:1669	nov	1667:1669	nov. (=KACC 19907T=NBRC 113666T) are proposed.					
35699986	9	26	theme	physiological	1264:1276	arg1	differences					1311:1321	distinct morphological, physiological, chemotaxonomic and phylogenetic differences	1240:1321	distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours	1240:1364	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	5	27	theme	genus	566:570	arg1	Mucilaginibacter					572:587	the genus Mucilaginibacter	562:587	the genus Mucilaginibacter	562:587	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	11	28	theme	pallidiroseus	1649:1661	arg1	sp					1663:1664	pallidiroseus sp	1649:1664	pallidiroseus sp	1649:1664	nov. (=KACC 19906T=NBRC 113667T) and Mucilaginibacter pallidiroseus sp.					
35699986	4	29	theme	chitinolytic	201:212	arg1	strains					280:286	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains	195:286	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T)	195:332	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	4	30	dep	chitinolytic	201:212	arg1	rod-shaped					259:268	rod-shaped	259:268	rod-shaped	259:268	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	4	30	dep	chitinolytic	201:212	arg1	capsule-forming					242:256	capsule-forming	242:256	capsule-forming	242:256	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	4	30	dep	chitinolytic	201:212	arg1	Gram-negative					215:227	Gram-negative	215:227	Gram-negative	215:227	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	4	30	dep	chitinolytic	201:212	arg1	pink					236:239	pink	236:239	pink	236:239	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	5	31	theme	Mucilaginibacter	572:587	arg1	species					551:557	novel species	545:557	novel species of the genus Mucilaginibacter	545:587	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	7	32	theme	coding	847:852	arg1	gene					854:857	the CAZys coding gene	837:857	the CAZys coding gene of KAAS	837:865	According to the CAZys coding gene of KAAS, MYSH2T and MJ1aT were interpreted as strains containing both GH18 and 19 family coding genes, except for dk17T, which shows only GH19 family genes.					
35699986	4	33	theme	gliding	293:299	arg1	motion					301:306	gliding motion	293:306	gliding motion (MYSH2T, MJ1aT and dk17T)	293:332	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	11	34	dep	sp	1663:1664	arg1	113667T					1619:1625	=KACC 19906T=NBRC 113667T	1601:1625	=KACC 19906T=NBRC 113667T	1601:1625	nov. (=KACC 19906T=NBRC 113667T) and Mucilaginibacter pallidiroseus sp.					
35699986	11	34	dep	sp	1663:1664	arg1	nov					1595:1597	nov	1595:1597	nov	1595:1597	nov. (=KACC 19906T=NBRC 113667T) and Mucilaginibacter pallidiroseus sp.					
35699986	11	34	dep	sp	1663:1664	arg1	Mucilaginibacter					1632:1647	Mucilaginibacter	1632:1647	Mucilaginibacter	1632:1647	nov. (=KACC 19906T=NBRC 113667T) and Mucilaginibacter pallidiroseus sp.					
35699986	0	35	theme	conchicola	35:44	arg1	sp					46:47	Mucilaginibacter conchicola sp	18:47	Mucilaginibacter conchicola sp	18:47	Identification of Mucilaginibacter conchicola sp.					
35699986	9	36	theme	chemotaxonomic	1279:1292	arg1	differences					1311:1321	distinct morphological, physiological, chemotaxonomic and phylogenetic differences	1240:1321	distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours	1240:1364	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	9	37	dep	strains	1383:1389	arg1	dk17T					1409:1413	dk17T	1409:1413	dk17T	1409:1413	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	9	37	dep	strains	1383:1389	arg1	strains					1383:1389	strains	1383:1389	strains MYSH2T, MJ1aT and dk17T	1383:1413	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	9	37	dep	strains	1383:1389	arg1	MJ1aT					1399:1403	MJ1aT	1399:1403	MJ1aT	1399:1403	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	9	37	dep	strains	1383:1389	arg1	MYSH2T					1391:1396	MYSH2T	1391:1396	MYSH2T	1391:1396	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	3	38	theme	Mucilaginibacter	177:192	arg1	nov.					135:138	nov.	135:138	nov.	135:138	nov. and emended description of the genus Mucilaginibacter.					
35699986	3	38	theme	Mucilaginibacter	177:192	arg1	description					152:162	emended description	144:162	emended description	144:162	nov. and emended description of the genus Mucilaginibacter.					
35699986	9	39	theme	closest	1334:1340	arg1	neighbours					1355:1364	their closest phylogenetic neighbours	1328:1364	their closest phylogenetic neighbours	1328:1364	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	0	40	theme	Mucilaginibacter	18:33	arg1	sp					46:47	Mucilaginibacter conchicola sp	18:47	Mucilaginibacter conchicola sp	18:47	Identification of Mucilaginibacter conchicola sp.					
35699986	9	41	from	species	1437:1443	arg1	Mucilaginibacter					1458:1473	the genus Mucilaginibacter	1448:1473	the genus Mucilaginibacter	1448:1473	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	5	42	theme	core	482:485	arg1	genes					487:491	92 core genes	479:491	92 core genes	479:491	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	7	43	theme	family	1002:1007	arg1	genes					1009:1013	only GH19 family genes	992:1013	only GH19 family genes	992:1013	According to the CAZys coding gene of KAAS, MYSH2T and MJ1aT were interpreted as strains containing both GH18 and 19 family coding genes, except for dk17T, which shows only GH19 family genes.					
35699986	9	44	theme	phylogenetic	1298:1309	arg1	differences					1311:1321	distinct morphological, physiological, chemotaxonomic and phylogenetic differences	1240:1321	distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours	1240:1364	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	9	45	theme	novel	1431:1435	arg1	species					1437:1443	three novel species	1425:1443	three novel species	1425:1443	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	5	46	theme	genes	487:491	arg1	analysis					409:416	Phylogenetic analysis	396:416	Phylogenetic analysis of the 16S rRNA gene sequences	396:447	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	5	46	theme	genes	487:491	arg1	alignment					466:474	concatenated alignment	453:474	concatenated alignment of 92 core genes	453:491	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	8	47	theme	chitin	1211:1216	arg1	degradation					1218:1228	industrial chitin degradation	1200:1228	industrial chitin degradation	1200:1228	These strains likely degrade chitin to chitobiose or directly to N-acetyl-d-glucosamine, which may enhance their chitinolytic capacity, thus making these stains potentially useful for industrial chitin degradation.					
35699986	0	48	theme	sp	46:47	arg1	Identification					0:13	Identification	0:13	Identification of Mucilaginibacter conchicola sp.	0:48	Identification of Mucilaginibacter conchicola sp.					
35699986	6	49	contain	contained	701:709	arg2	MK-7					771:774	MK-7	771:774	MK-7	771:774	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	49	contain	contained	701:709	arg1	strains					693:699	These novel strains	681:699	These novel strains	681:699	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	49	contain	contained	701:709	arg2	feature					718:724	summed feature 3	711:726	summed feature 3 (C16:1 ω7c and/or C16:1 ω6)	711:754	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	49	contain	contained	701:709	arg2	iso-C15:0					757:765	iso-C15:0	757:765	iso-C15:0	757:765	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	49	contain	contained	701:709	arg2	acids					801:805	the predominant fatty acids	779:805	the predominant fatty acids	779:805	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	10	50	theme	19716T=JCM	1540:1549	arg1	32787T					1551:1556	=KACC 19716T=JCM 32787T	1534:1556	=KACC 19716T=JCM 32787T	1534:1556	nov. (=KACC 19716T=JCM 32787T), Mucilaginibacter achroorhodeus sp.					
35699986	10	50	theme	19716T=JCM	1540:1549	arg1	nov					1528:1530	nov	1528:1530	nov	1528:1530	nov. (=KACC 19716T=JCM 32787T), Mucilaginibacter achroorhodeus sp.					
35699986	5	51	dep	strains	508:514	arg1	dk17T					534:538	dk17T	534:538	dk17T	534:538	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	5	51	dep	strains	508:514	arg1	strains					508:514	strains	508:514	strains MYSH2T, MJ1aT and dk17T	508:538	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	5	51	dep	strains	508:514	arg1	MJ1aT					524:528	MJ1aT	524:528	MJ1aT	524:528	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	5	51	dep	strains	508:514	arg1	MYSH2T					516:521	MYSH2T	516:521	MYSH2T	516:521	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	8	52	theme	useful	1189:1194	arg1	stains					1170:1175	these stains	1164:1175	these stains potentially useful for industrial chitin degradation	1164:1228	These strains likely degrade chitin to chitobiose or directly to N-acetyl-d-glucosamine, which may enhance their chitinolytic capacity, thus making these stains potentially useful for industrial chitin degradation.					
35699986	10	53	theme	Mucilaginibacter	1560:1575	arg1	nov					1528:1530	nov	1528:1530	nov	1528:1530	nov. (=KACC 19716T=JCM 32787T), Mucilaginibacter achroorhodeus sp.					
35699986	10	53	theme	Mucilaginibacter	1560:1575	arg1	sp					1591:1592	Mucilaginibacter achroorhodeus sp	1560:1592	Mucilaginibacter achroorhodeus sp	1560:1592	nov. (=KACC 19716T=JCM 32787T), Mucilaginibacter achroorhodeus sp.					
35699986	2	54	dep	sp	131:132	arg1	pallidiroseus					117:129	nov. and Mucilaginibacter pallidiroseus	91:129	nov. and Mucilaginibacter pallidiroseus	91:129	nov. and Mucilaginibacter pallidiroseus sp.					
35699986	8	55	theme	chitinolytic	1129:1140	arg1	capacity					1142:1149	their chitinolytic capacity	1123:1149	their chitinolytic capacity	1123:1149	These strains likely degrade chitin to chitobiose or directly to N-acetyl-d-glucosamine, which may enhance their chitinolytic capacity, thus making these stains potentially useful for industrial chitin degradation.					
35699986	13	56	theme	Mucilaginibacter	1750:1765	arg1	description					1725:1735	An emended description	1714:1735	An emended description of the genus Mucilaginibacter	1714:1765	An emended description of the genus Mucilaginibacter is proposed.					
35699986	4	57	dep	motion	301:306	arg1	dk17T					327:331	dk17T	327:331	dk17T	327:331	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	4	57	dep	motion	301:306	arg1	MJ1aT					317:321	MJ1aT	317:321	MJ1aT	317:321	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	4	57	dep	motion	301:306	arg1	MYSH2T					309:314	MYSH2T	309:314	MYSH2T	309:314	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	6	58	theme	fatty	795:799	arg1	iso-C15:0					757:765	iso-C15:0	757:765	iso-C15:0	757:765	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	58	theme	fatty	795:799	arg1	feature					718:724	summed feature 3	711:726	summed feature 3 (C16:1 ω7c and/or C16:1 ω6)	711:754	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	58	theme	fatty	795:799	arg1	acids					801:805	the predominant fatty acids	779:805	the predominant fatty acids	779:805	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	58	theme	fatty	795:799	arg1	MK-7					771:774	MK-7	771:774	MK-7	771:774	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	5	59	theme	sequences	439:447	arg1	analysis					409:416	Phylogenetic analysis	396:416	Phylogenetic analysis of the 16S rRNA gene sequences	396:447	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	5	59	theme	sequences	439:447	arg1	alignment					466:474	concatenated alignment	453:474	concatenated alignment of 92 core genes	453:491	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	2	60	theme	nov.	91:94	arg1	pallidiroseus					117:129	nov. and Mucilaginibacter pallidiroseus	91:129	nov. and Mucilaginibacter pallidiroseus	91:129	nov. and Mucilaginibacter pallidiroseus sp.					
35699986	6	61	theme	predominant	783:793	arg1	iso-C15:0					757:765	iso-C15:0	757:765	iso-C15:0	757:765	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	61	theme	predominant	783:793	arg1	feature					718:724	summed feature 3	711:726	summed feature 3 (C16:1 ω7c and/or C16:1 ω6)	711:754	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	61	theme	predominant	783:793	arg1	acids					801:805	the predominant fatty acids	779:805	the predominant fatty acids	779:805	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	61	theme	predominant	783:793	arg1	MK-7					771:774	MK-7	771:774	MK-7	771:774	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	7	62	theme	family	941:946	arg1	genes					955:959	both GH18 and 19 family coding genes	924:959	genes	955:959	According to the CAZys coding gene of KAAS, MYSH2T and MJ1aT were interpreted as strains containing both GH18 and 19 family coding genes, except for dk17T, which shows only GH19 family genes.					
35699986	9	63	theme	genus	1452:1456	arg1	Mucilaginibacter					1458:1473	the genus Mucilaginibacter	1448:1473	the genus Mucilaginibacter	1448:1473	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	7	64	theme	KAAS	862:865	arg1	gene					854:857	the CAZys coding gene	837:857	the CAZys coding gene of KAAS	837:865	According to the CAZys coding gene of KAAS, MYSH2T and MJ1aT were interpreted as strains containing both GH18 and 19 family coding genes, except for dk17T, which shows only GH19 family genes.					
35699986	9	65	theme	phylogenetic	1342:1353	arg1	neighbours					1355:1364	their closest phylogenetic neighbours	1328:1364	their closest phylogenetic neighbours	1328:1364	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	9	66	from	neighbours	1355:1364	arg1	differences					1311:1321	distinct morphological, physiological, chemotaxonomic and phylogenetic differences	1240:1321	distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours	1240:1364	Based on distinct morphological, physiological, chemotaxonomic and phylogenetic differences from their closest phylogenetic neighbours, we propose that strains MYSH2T, MJ1aT and dk17T represent three novel species in the genus Mucilaginibacter, for which the names Mucilaginibacter conchicola sp.					
35699986	7	67	theme	coding	948:953	arg1	genes					955:959	both GH18 and 19 family coding genes	924:959	genes	955:959	According to the CAZys coding gene of KAAS, MYSH2T and MJ1aT were interpreted as strains containing both GH18 and 19 family coding genes, except for dk17T, which shows only GH19 family genes.					
35699986	5	68	theme	concatenated	453:464	arg1	alignment					466:474	concatenated alignment	453:474	concatenated alignment of 92 core genes	453:491	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	5	69	theme	Phylogenetic	396:407	arg1	analysis					409:416	Phylogenetic analysis	396:416	Phylogenetic analysis of the 16S rRNA gene sequences	396:447	Phylogenetic analysis of the 16S rRNA gene sequences and concatenated alignment of 92 core genes indicated that strains MYSH2T, MJ1aT and dk17T were novel species of the genus Mucilaginibacter and exhibited a high 16S rRNA sequence similarity (i.e. more than 97.2 %) among each other.					
35699986	13	70	theme	genus	1744:1748	arg1	Mucilaginibacter					1750:1765	the genus Mucilaginibacter	1740:1765	the genus Mucilaginibacter	1740:1765	An emended description of the genus Mucilaginibacter is proposed.					
35699986	8	71	theme	industrial	1200:1209	arg1	degradation					1218:1228	industrial chitin degradation	1200:1228	industrial chitin degradation	1200:1228	These strains likely degrade chitin to chitobiose or directly to N-acetyl-d-glucosamine, which may enhance their chitinolytic capacity, thus making these stains potentially useful for industrial chitin degradation.					
35699986	6	72	theme	summed	711:716	arg1	iso-C15:0					757:765	iso-C15:0	757:765	iso-C15:0	757:765	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	72	theme	summed	711:716	arg1	feature					718:724	summed feature 3	711:726	summed feature 3 (C16:1 ω7c and/or C16:1 ω6)	711:754	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	72	theme	summed	711:716	arg1	acids					801:805	the predominant fatty acids	779:805	the predominant fatty acids	779:805	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	6	72	theme	summed	711:716	arg1	MK-7					771:774	MK-7	771:774	MK-7	771:774	These novel strains contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6), iso-C15:0 and MK-7 as the predominant fatty acids and menaquinone.					
35699986	4	73	theme	bacterial	270:278	arg1	strains					280:286	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains	195:286	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T)	195:332	Three chitinolytic, Gram-negative, light pink, capsule-forming, rod-shaped bacterial strains with gliding motion (MYSH2T, MJ1aT and dk17T) were isolated from seashells, soil and foxtail, respectively.					
35699986	7	74	contain	containing	913:922	arg2	genes					955:959	both GH18 and 19 family coding genes	924:959	genes	955:959	According to the CAZys coding gene of KAAS, MYSH2T and MJ1aT were interpreted as strains containing both GH18 and 19 family coding genes, except for dk17T, which shows only GH19 family genes.					
35699986	7	74	contain	containing	913:922	arg2	GH18					929:932	both GH18 and 19 family coding genes	924:959	GH18	929:932	According to the CAZys coding gene of KAAS, MYSH2T and MJ1aT were interpreted as strains containing both GH18 and 19 family coding genes, except for dk17T, which shows only GH19 family genes.					
35699986	7	74	contain	containing	913:922	arg1	MYSH2T					868:873	MYSH2T	868:873	MYSH2T	868:873	According to the CAZys coding gene of KAAS, MYSH2T and MJ1aT were interpreted as strains containing both GH18 and 19 family coding genes, except for dk17T, which shows only GH19 family genes.					
35699986	7	74	contain	containing	913:922	arg1	MJ1aT					879:883	MJ1aT	879:883	MJ1aT	879:883	According to the CAZys coding gene of KAAS, MYSH2T and MJ1aT were interpreted as strains containing both GH18 and 19 family coding genes, except for dk17T, which shows only GH19 family genes.					
35699986	7	74	contain	containing	913:922	arg1	strains					905:911	strains	905:911	strains containing both GH18 and 19 family coding genes	905:959	According to the CAZys coding gene of KAAS, MYSH2T and MJ1aT were interpreted as strains containing both GH18 and 19 family coding genes, except for dk17T, which shows only GH19 family genes.					
35699986	10	75	theme	=KACC	1534:1538	arg1	32787T					1551:1556	=KACC 19716T=JCM 32787T	1534:1556	=KACC 19716T=JCM 32787T	1534:1556	nov. (=KACC 19716T=JCM 32787T), Mucilaginibacter achroorhodeus sp.					
35699986	10	75	theme	=KACC	1534:1538	arg1	nov					1528:1530	nov	1528:1530	nov	1528:1530	nov. (=KACC 19716T=JCM 32787T), Mucilaginibacter achroorhodeus sp.					
35699986	3	76	theme	emended	144:150	arg1	description					152:162	emended description	144:162	emended description	144:162	nov. and emended description of the genus Mucilaginibacter.					
35699986	10	77	theme	achroorhodeus	1577:1589	arg1	nov					1528:1530	nov	1528:1530	nov	1528:1530	nov. (=KACC 19716T=JCM 32787T), Mucilaginibacter achroorhodeus sp.					
35699986	10	77	theme	achroorhodeus	1577:1589	arg1	sp					1591:1592	Mucilaginibacter achroorhodeus sp	1560:1592	Mucilaginibacter achroorhodeus sp	1560:1592	nov. (=KACC 19716T=JCM 32787T), Mucilaginibacter achroorhodeus sp.					
35699986	2	78	theme	Mucilaginibacter	100:115	arg1	pallidiroseus					117:129	nov. and Mucilaginibacter pallidiroseus	91:129	nov. and Mucilaginibacter pallidiroseus	91:129	nov. and Mucilaginibacter pallidiroseus sp.					
36529123	10	0	theme	intense	1729:1735	arg1	fluorescence					1741:1752	intense red fluorescence	1729:1752	intense red fluorescence	1729:1752	A. naeslundii and P. intermedia emitted intense red fluorescence when grown on caso or caso blood agar but not on caso chlorophyll agar.					
36529123	10	1	theme	caso	1768:1771	arg1	agar					1787:1790	caso or caso blood agar	1768:1790	agar	1787:1790	A. naeslundii and P. intermedia emitted intense red fluorescence when grown on caso or caso blood agar but not on caso chlorophyll agar.					
36529123	11	2	theme	Fluorescence	1826:1837	arg1	spectra					1848:1854	Fluorescence emission spectra	1826:1854	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar	1826:1914	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar correlated exactly with both fluorescence peaks for protoporphyrin-IX at 632 and 701nm.					
36529123	1	3	theme	infected	285:292	arg1	tissue					294:299	bacterially infected tissue	273:299	bacterially infected tissue	273:299	Red fluorophores synthesized by oral bacteria are important for fluorescence-based diagnosis and treatment because they are used as markers for bacterially infected tissue, mature plaque or calculus.					
36529123	11	4	from	632	1989:1991	arg1	peaks					1958:1962	both fluorescence peaks	1940:1962	both fluorescence peaks for protoporphyrin-IX at 632 and 701nm	1940:2001	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar correlated exactly with both fluorescence peaks for protoporphyrin-IX at 632 and 701nm.					
36529123	11	5	theme	A.	1859:1860	arg1	spectra					1848:1854	Fluorescence emission spectra	1826:1854	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar	1826:1914	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar correlated exactly with both fluorescence peaks for protoporphyrin-IX at 632 and 701nm.					
36529123	1	6	theme	fluorescence-based	193:210	arg1	diagnosis					212:220	fluorescence-based diagnosis	193:220	fluorescence-based diagnosis	193:220	Red fluorophores synthesized by oral bacteria are important for fluorescence-based diagnosis and treatment because they are used as markers for bacterially infected tissue, mature plaque or calculus.					
36529123	11	7	theme	P.	1877:1878	arg1	spectra					1848:1854	Fluorescence emission spectra	1826:1854	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar	1826:1914	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar correlated exactly with both fluorescence peaks for protoporphyrin-IX at 632 and 701nm.					
36529123	4	8	theme	selected	628:635	arg1	bacteria					651:658	selected cultured oral bacteria	628:658	selected cultured oral bacteria	628:658	This study examined and compared the fluorescence spectra produced by selected cultured oral bacteria when grown on agars containing different nutrients with spectra for protoporphyrin IX, Zn-protoporphyrin IX, haematoporphyrin and haematin.					
36529123	10	9	theme	caso	1776:1779	arg1	agar					1787:1790	caso or caso blood agar	1768:1790	agar	1787:1790	A. naeslundii and P. intermedia emitted intense red fluorescence when grown on caso or caso blood agar but not on caso chlorophyll agar.					
36529123	5	10	contain	containing	1220:1229	arg1	blood					1213:1217	caso blood	1208:1217	caso blood (containing 5% sheep blood)	1208:1245	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	5	10	contain	containing	1220:1229	arg2	blood					1240:1244	5% sheep blood	1231:1244	5% sheep blood	1231:1244	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	5	11	theme	Mikroorganismen	849:863	arg1	43320					866:870	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742)	800:1157	43320	866:870	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	4	12	theme	oral	646:649	arg1	bacteria					651:658	selected cultured oral bacteria	628:658	selected cultured oral bacteria	628:658	This study examined and compared the fluorescence spectra produced by selected cultured oral bacteria when grown on agars containing different nutrients with spectra for protoporphyrin IX, Zn-protoporphyrin IX, haematoporphyrin and haematin.					
36529123	10	13	theme	caso	1803:1806	arg1	agar					1820:1823	caso chlorophyll agar	1803:1823	caso chlorophyll agar	1803:1823	A. naeslundii and P. intermedia emitted intense red fluorescence when grown on caso or caso blood agar but not on caso chlorophyll agar.					
36529123	11	14	theme	caso	1900:1903	arg1	agar					1911:1914	caso blood agar	1900:1914	caso blood agar	1900:1914	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar correlated exactly with both fluorescence peaks for protoporphyrin-IX at 632 and 701nm.					
36529123	12	15	theme	IX	2124:2125	arg1	emission-peaks					2069:2082	the emission-peaks	2065:2082	the emission-peaks of protoporphyrin IX, Zn-protoporphyrin IX or haematoporphyrin	2065:2145	Most peaks observed could be correlated with at least one of the emission-peaks of protoporphyrin IX, Zn-protoporphyrin IX or haematoporphyrin.					
36529123	5	16	dep	Lactobacillus	947:959	arg1	casei					961:965	Lactobacillus casei (DSM 20011)	947:977	Lactobacillus casei (DSM 20011)	947:977	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	5	17	theme	caso	1208:1211	arg1	blood					1213:1217	caso blood	1208:1217	caso blood (containing 5% sheep blood)	1208:1245	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	7	18	dep	recorded	1439:1446	arg1	Fluorolog					1490:1498	Fluorolog	1490:1498	Fluorolog	1490:1498	Fluorescence emission spectra were recorded from the supernatant at 405nm excitation (Fluorolog 3-22.					
36529123	7	19	theme	405nm	1472:1476	arg1	excitation					1478:1487	405nm excitation	1472:1487	405nm excitation	1472:1487	Fluorescence emission spectra were recorded from the supernatant at 405nm excitation (Fluorolog 3-22.					
36529123	12	20	theme	haematoporphyrin	2130:2145	arg1	emission-peaks					2069:2082	the emission-peaks	2065:2082	the emission-peaks of protoporphyrin IX, Zn-protoporphyrin IX or haematoporphyrin	2065:2145	Most peaks observed could be correlated with at least one of the emission-peaks of protoporphyrin IX, Zn-protoporphyrin IX or haematoporphyrin.					
36529123	12	21	theme	protoporphyrin	2087:2100	arg1	IX					2102:2103	protoporphyrin IX	2087:2103	protoporphyrin IX	2087:2103	Most peaks observed could be correlated with at least one of the emission-peaks of protoporphyrin IX, Zn-protoporphyrin IX or haematoporphyrin.					
36529123	5	22	theme	caso	1202:1205	arg1	agar					1300:1303	caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar	1202:1303	caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar	1202:1303	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	4	23	theme	protoporphyrin	728:741	arg1	IX					743:744	protoporphyrin IX	728:744	protoporphyrin IX	728:744	This study examined and compared the fluorescence spectra produced by selected cultured oral bacteria when grown on agars containing different nutrients with spectra for protoporphyrin IX, Zn-protoporphyrin IX, haematoporphyrin and haematin.					
36529123	2	24	theme	fluorescence	393:404	arg1	source					378:383	the source	374:383	the source of this fluorescence in carious tissue	374:422	A range of porphyrins has been identified as the source of this fluorescence in carious tissue.					
36529123	2	24	theme	fluorescence	393:404	arg1	range					331:335	A range	329:335	A range of porphyrins	329:349	A range of porphyrins has been identified as the source of this fluorescence in carious tissue.					
36529123	0	25	theme	growth	114:119	arg1	media					121:125	growth media	114:125	growth media	114:125	[Fluorescence spectroscopy shows porphyrins produced by cultured oral bacteria differ depending on composition of growth media.]					
36529123	9	26	theme	caso	1638:1641	arg1	agar					1655:1658	caso chlorophyll agar	1638:1658	caso chlorophyll agar	1638:1658	All streptococci, L. casei and F. nucleatum produced red fluorescence when grown on caso and caso chlorophyll agar, but not on caso blood agar.					
36529123	12	27	theme	emission-peaks	2069:2082	arg1	emission-peaks					2069:2082	the emission-peaks	2065:2082	the emission-peaks of protoporphyrin IX, Zn-protoporphyrin IX or haematoporphyrin	2065:2145	Most peaks observed could be correlated with at least one of the emission-peaks of protoporphyrin IX, Zn-protoporphyrin IX or haematoporphyrin.					
36529123	12	27	theme	emission-peaks	2069:2082	arg1	one					2058:2060	one	2058:2060	one	2058:2060	Most peaks observed could be correlated with at least one of the emission-peaks of protoporphyrin IX, Zn-protoporphyrin IX or haematoporphyrin.					
36529123	5	28	theme	caso	1251:1254	arg1	chlorophyll					1256:1266	caso chlorophyll	1251:1266	caso chlorophyll (containing 5% spinach extract)	1251:1298	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	0	29	theme	[Fluorescence	0:12	arg1	spectroscopy					14:25	[Fluorescence spectroscopy	0:25	[Fluorescence spectroscopy	0:25	[Fluorescence spectroscopy shows porphyrins produced by cultured oral bacteria differ depending on composition of growth media.]					
36529123	5	30	theme	5	1231:1231	arg1	%					1232:1232	%	1232:1232	%	1232:1232	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	5	31	theme	Sammlung	836:843	arg1	43320					866:870	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742)	800:1157	43320	866:870	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	1	32	theme	mature	302:307	arg1	plaque					309:314	mature plaque	302:314	mature plaque	302:314	Red fluorophores synthesized by oral bacteria are important for fluorescence-based diagnosis and treatment because they are used as markers for bacterially infected tissue, mature plaque or calculus.					
36529123	1	32	theme	mature	302:307	arg1	markers					261:267	markers	261:267	markers for bacterially infected tissue	261:299	Red fluorophores synthesized by oral bacteria are important for fluorescence-based diagnosis and treatment because they are used as markers for bacterially infected tissue, mature plaque or calculus.					
36529123	7	33	theme	Fluorescence	1404:1415	arg1	spectra					1426:1432	Fluorescence emission spectra	1404:1432	Fluorescence emission spectra	1404:1432	Fluorescence emission spectra were recorded from the supernatant at 405nm excitation (Fluorolog 3-22.					
36529123	0	34	dep	bacteria	70:77	arg1	differ					79:84	differ	79:84	differ depending on composition of growth media	79:125	[Fluorescence spectroscopy shows porphyrins produced by cultured oral bacteria differ depending on composition of growth media.]					
36529123	5	35	theme	sheep	1234:1238	arg1	blood					1240:1244	5% sheep blood	1231:1244	5% sheep blood	1231:1244	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	2	36	theme	carious	409:415	arg1	tissue					417:422	carious tissue	409:422	carious tissue	409:422	A range of porphyrins has been identified as the source of this fluorescence in carious tissue.					
36529123	5	37	theme	Actinomyces	800:810	arg1	43320					866:870	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742)	800:1157	43320	866:870	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	5	38	theme	spinach	1283:1289	arg1	extract					1291:1297	5% spinach extract	1280:1297	5% spinach extract	1280:1297	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	0	39	theme	cultured	56:63	arg1	bacteria					70:77	cultured oral bacteria	56:77	cultured oral bacteria differ depending on composition of growth media	56:125	[Fluorescence spectroscopy shows porphyrins produced by cultured oral bacteria differ depending on composition of growth media.]					
36529123	9	40	theme	blood	1677:1681	arg1	agar					1683:1686	caso blood agar	1672:1686	caso blood agar	1672:1686	All streptococci, L. casei and F. nucleatum produced red fluorescence when grown on caso and caso chlorophyll agar, but not on caso blood agar.					
36529123	5	41	theme	israelii	812:819	arg1	43320					866:870	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742)	800:1157	43320	866:870	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	11	42	dep	P.	1877:1878	arg1	intermedia					1880:1889	P. intermedia grown on caso blood agar	1877:1914	P. intermedia grown on caso blood agar	1877:1914	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar correlated exactly with both fluorescence peaks for protoporphyrin-IX at 632 and 701nm.					
36529123	11	42	dep	P.	1877:1878	arg1	grown					1891:1895	grown	1891:1895	grown on caso blood agar	1891:1914	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar correlated exactly with both fluorescence peaks for protoporphyrin-IX at 632 and 701nm.					
36529123	10	43	theme	red	1737:1739	arg1	fluorescence					1741:1752	intense red fluorescence	1729:1752	intense red fluorescence	1729:1752	A. naeslundii and P. intermedia emitted intense red fluorescence when grown on caso or caso blood agar but not on caso chlorophyll agar.					
36529123	5	44	theme	5	1280:1280	arg1	%					1281:1281	%	1281:1281	%	1281:1281	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	3	45	theme	oral	494:497	arg1	bacteria					499:506	individual oral bacteria	483:506	individual oral bacteria	483:506	It is not clear which of these porphyrins are produced by individual oral bacteria or whether this ability depends on other factors.					
36529123	4	46	contain	containing	680:689	arg1	agars					674:678	agars	674:678	agars containing different nutrients with spectra for protoporphyrin IX, Zn-protoporphyrin IX, haematoporphyrin and haematin	674:797	This study examined and compared the fluorescence spectra produced by selected cultured oral bacteria when grown on agars containing different nutrients with spectra for protoporphyrin IX, Zn-protoporphyrin IX, haematoporphyrin and haematin.					
36529123	4	46	contain	containing	680:689	arg2	nutrients					701:709	different nutrients	691:709	different nutrients	691:709	This study examined and compared the fluorescence spectra produced by selected cultured oral bacteria when grown on agars containing different nutrients with spectra for protoporphyrin IX, Zn-protoporphyrin IX, haematoporphyrin and haematin.					
36529123	13	47	theme	Oral	2148:2151	arg1	bacteria					2153:2160	Oral bacteria	2148:2160	Oral bacteria	2148:2160	Oral bacteria emitted red fluorescence matching known porphyrins, but this depended on nutrients available in the agar.					
36529123	11	48	theme	fluorescence	1945:1956	arg1	peaks					1958:1962	both fluorescence peaks	1940:1962	both fluorescence peaks for protoporphyrin-IX at 632 and 701nm	1940:2001	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar correlated exactly with both fluorescence peaks for protoporphyrin-IX at 632 and 701nm.					
36529123	10	49	theme	blood	1781:1785	arg1	agar					1787:1790	caso or caso blood agar	1768:1790	agar	1787:1790	A. naeslundii and P. intermedia emitted intense red fluorescence when grown on caso or caso blood agar but not on caso chlorophyll agar.					
36529123	3	50	theme	other	543:547	arg1	factors					549:555	other factors	543:555	other factors	543:555	It is not clear which of these porphyrins are produced by individual oral bacteria or whether this ability depends on other factors.					
36529123	5	51	theme	von	845:847	arg1	43320					866:870	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742)	800:1157	43320	866:870	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	5	52	dep	Prevotella	980:989	arg1	intermedia					991:1000	Prevotella intermedia	980:1000	Prevotella intermedia (DSM 20706)	980:1012	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	2	53	theme	porphyrins	340:349	arg1	range					331:335	A range	329:335	A range of porphyrins	329:349	A range of porphyrins has been identified as the source of this fluorescence in carious tissue.					
36529123	2	53	theme	porphyrins	340:349	arg1	source					378:383	the source	374:383	the source of this fluorescence in carious tissue	374:422	A range of porphyrins has been identified as the source of this fluorescence in carious tissue.					
36529123	13	54	theme	red	2170:2172	arg1	fluorescence					2174:2185	red fluorescence	2170:2185	red fluorescence matching known porphyrins	2170:2211	Oral bacteria emitted red fluorescence matching known porphyrins, but this depended on nutrients available in the agar.					
36529123	4	55	theme	cultured	637:644	arg1	bacteria					651:658	selected cultured oral bacteria	628:658	selected cultured oral bacteria	628:658	This study examined and compared the fluorescence spectra produced by selected cultured oral bacteria when grown on agars containing different nutrients with spectra for protoporphyrin IX, Zn-protoporphyrin IX, haematoporphyrin and haematin.					
36529123	11	56	theme	blood	1905:1909	arg1	agar					1911:1914	caso blood agar	1900:1914	caso blood agar	1900:1914	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar correlated exactly with both fluorescence peaks for protoporphyrin-IX at 632 and 701nm.					
36529123	13	57	from	available	2245:2253	arg1	agar					2262:2265	the agar	2258:2265	the agar	2258:2265	Oral bacteria emitted red fluorescence matching known porphyrins, but this depended on nutrients available in the agar.					
36529123	1	58	theme	Red	129:131	arg1	fluorophores					133:144	Red fluorophores	129:144	Red fluorophores synthesized by oral bacteria	129:173	Red fluorophores synthesized by oral bacteria are important for fluorescence-based diagnosis and treatment because they are used as markers for bacterially infected tissue, mature plaque or calculus.					
36529123	11	59	theme	emission	1839:1846	arg1	spectra					1848:1854	Fluorescence emission spectra	1826:1854	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar	1826:1914	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar correlated exactly with both fluorescence peaks for protoporphyrin-IX at 632 and 701nm.					
36529123	5	60	theme	blood	1213:1217	arg1	agar					1300:1303	caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar	1202:1303	caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar	1202:1303	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	13	61	from	agar	2262:2265	arg1	available					2245:2253	available	2245:2253	available	2245:2253	Oral bacteria emitted red fluorescence matching known porphyrins, but this depended on nutrients available in the agar.					
36529123	9	62	theme	red	1598:1600	arg1	fluorescence					1602:1613	red fluorescence	1598:1613	red fluorescence	1598:1613	All streptococci, L. casei and F. nucleatum produced red fluorescence when grown on caso and caso chlorophyll agar, but not on caso blood agar.					
36529123	12	63	theme	IX	2102:2103	arg1	emission-peaks					2069:2082	the emission-peaks	2065:2082	the emission-peaks of protoporphyrin IX, Zn-protoporphyrin IX or haematoporphyrin	2065:2145	Most peaks observed could be correlated with at least one of the emission-peaks of protoporphyrin IX, Zn-protoporphyrin IX or haematoporphyrin.					
36529123	10	64	theme	chlorophyll	1808:1818	arg1	agar					1820:1823	caso chlorophyll agar	1803:1823	caso chlorophyll agar	1803:1823	A. naeslundii and P. intermedia emitted intense red fluorescence when grown on caso or caso blood agar but not on caso chlorophyll agar.					
36529123	1	65	theme	oral	161:164	arg1	bacteria					166:173	oral bacteria	161:173	oral bacteria	161:173	Red fluorophores synthesized by oral bacteria are important for fluorescence-based diagnosis and treatment because they are used as markers for bacterially infected tissue, mature plaque or calculus.					
36529123	12	66	theme	Zn-protoporphyrin	2106:2122	arg1	IX					2124:2125	Zn-protoporphyrin IX	2106:2125	Zn-protoporphyrin IX	2106:2125	Most peaks observed could be correlated with at least one of the emission-peaks of protoporphyrin IX, Zn-protoporphyrin IX or haematoporphyrin.					
36529123	4	67	theme	different	691:699	arg1	nutrients					701:709	different nutrients	691:709	different nutrients	691:709	This study examined and compared the fluorescence spectra produced by selected cultured oral bacteria when grown on agars containing different nutrients with spectra for protoporphyrin IX, Zn-protoporphyrin IX, haematoporphyrin and haematin.					
36529123	9	68	dep	F.	1576:1577	arg1	nucleatum					1579:1587	F. nucleatum	1576:1587	F. nucleatum	1576:1587	All streptococci, L. casei and F. nucleatum produced red fluorescence when grown on caso and caso chlorophyll agar, but not on caso blood agar.					
36529123	0	69	theme	media	121:125	arg1	composition					99:109	composition	99:109	composition of growth media	99:125	[Fluorescence spectroscopy shows porphyrins produced by cultured oral bacteria differ depending on composition of growth media.]					
36529123	5	70	dep	Fusobacterium	910:922	arg1	nucleatum					924:932	Fusobacterium nucleatum (DSM 20482)	910:944	Fusobacterium nucleatum (DSM 20482)	910:944	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	5	71	theme	DSM	822:824	arg1	43320					866:870	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742)	800:1157	43320	866:870	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	9	72	theme	chlorophyll	1643:1653	arg1	agar					1655:1658	caso chlorophyll agar	1638:1658	caso chlorophyll agar	1638:1658	All streptococci, L. casei and F. nucleatum produced red fluorescence when grown on caso and caso chlorophyll agar, but not on caso blood agar.					
36529123	13	73	theme	available	2245:2253	arg1	nutrients					2235:2243	nutrients	2235:2243	nutrients available in the agar	2235:2265	Oral bacteria emitted red fluorescence matching known porphyrins, but this depended on nutrients available in the agar.					
36529123	4	74	theme	Zn-protoporphyrin	747:763	arg1	IX					765:766	Zn-protoporphyrin IX	747:766	Zn-protoporphyrin IX	747:766	This study examined and compared the fluorescence spectra produced by selected cultured oral bacteria when grown on agars containing different nutrients with spectra for protoporphyrin IX, Zn-protoporphyrin IX, haematoporphyrin and haematin.					
36529123	5	75	theme	%	1232:1232	arg1	blood					1240:1244	5% sheep blood	1231:1244	5% sheep blood	1231:1244	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	7	76	theme	emission	1417:1424	arg1	spectra					1426:1432	Fluorescence emission spectra	1404:1432	Fluorescence emission spectra	1404:1432	Fluorescence emission spectra were recorded from the supernatant at 405nm excitation (Fluorolog 3-22.					
36529123	5	77	theme	%	1281:1281	arg1	extract					1291:1297	5% spinach extract	1280:1297	5% spinach extract	1280:1297	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	0	78	theme	oral	65:68	arg1	bacteria					70:77	cultured oral bacteria	56:77	cultured oral bacteria differ depending on composition of growth media	56:125	[Fluorescence spectroscopy shows porphyrins produced by cultured oral bacteria differ depending on composition of growth media.]					
36529123	2	79	from	source	378:383	arg1	tissue					417:422	carious tissue	409:422	carious tissue	409:422	A range of porphyrins has been identified as the source of this fluorescence in carious tissue.					
36529123	9	80	theme	caso	1672:1675	arg1	agar					1683:1686	caso blood agar	1672:1686	caso blood agar	1672:1686	All streptococci, L. casei and F. nucleatum produced red fluorescence when grown on caso and caso chlorophyll agar, but not on caso blood agar.					
36529123	12	81	theme	Most	2004:2007	arg1	peaks					2009:2013	Most peaks	2004:2013	Most peaks observed	2004:2022	Most peaks observed could be correlated with at least one of the emission-peaks of protoporphyrin IX, Zn-protoporphyrin IX or haematoporphyrin.					
36529123	3	82	theme	individual	483:492	arg1	bacteria					499:506	individual oral bacteria	483:506	individual oral bacteria	483:506	It is not clear which of these porphyrins are produced by individual oral bacteria or whether this ability depends on other factors.					
36529123	1	83	used	used	253:256	arg2	they					244:247	they	244:247	they	244:247	Red fluorophores synthesized by oral bacteria are important for fluorescence-based diagnosis and treatment because they are used as markers for bacterially infected tissue, mature plaque or calculus.					
36529123	1	83	used	used	253:256	arg2	markers					261:267	markers	261:267	markers for bacterially infected tissue	261:299	Red fluorophores synthesized by oral bacteria are important for fluorescence-based diagnosis and treatment because they are used as markers for bacterially infected tissue, mature plaque or calculus.					
36529123	4	84	theme	fluorescence	595:606	arg1	spectra					608:614	the fluorescence spectra	591:614	the fluorescence spectra produced by selected cultured oral bacteria	591:658	This study examined and compared the fluorescence spectra produced by selected cultured oral bacteria when grown on agars containing different nutrients with spectra for protoporphyrin IX, Zn-protoporphyrin IX, haematoporphyrin and haematin.					
36529123	5	85	dep	43320	866:870	arg1	Actinomyces					874:884	Actinomyces	874:884	Actinomyces	874:884	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	5	85	dep	43320	866:870	arg1	Deutsche					827:834	Deutsche	827:834	Deutsche	827:834	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	5	85	dep	43320	866:870	arg1	DSM					898:900	DSM 43013	898:906	DSM 43013	898:906	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	5	85	dep	43320	866:870	arg1	Fusobacterium					910:922	Fusobacterium	910:922	Fusobacterium	910:922	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	5	86	theme	chlorophyll	1256:1266	arg1	agar					1300:1303	caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar	1202:1303	caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar	1202:1303	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	13	87	theme	known	2196:2200	arg1	porphyrins					2202:2211	known porphyrins	2196:2211	known porphyrins	2196:2211	Oral bacteria emitted red fluorescence matching known porphyrins, but this depended on nutrients available in the agar.					
36529123	11	88	from	701nm	1997:2001	arg1	peaks					1958:1962	both fluorescence peaks	1940:1962	both fluorescence peaks for protoporphyrin-IX at 632 and 701nm	1940:2001	Fluorescence emission spectra of A. naeslundii and P. intermedia grown on caso blood agar correlated exactly with both fluorescence peaks for protoporphyrin-IX at 632 and 701nm.					
36529123	5	89	contain	containing	1269:1278	arg1	chlorophyll					1256:1266	caso chlorophyll	1251:1266	caso chlorophyll (containing 5% spinach extract)	1251:1298	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
36529123	5	89	contain	containing	1269:1278	arg2	extract					1291:1297	5% spinach extract	1280:1297	5% spinach extract	1280:1297	Actinomyces israelii (DSM, Deutsche Sammlung von Mikroorganismen, 43320), Actinomyces naeslundii (DSM 43013), Fusobacterium nucleatum (DSM 20482), Lactobacillus casei (DSM 20011), Prevotella intermedia (DSM 20706), Streptococcus mutans (DSM 20523), Streptococcus oralis (DSM 20627), Streptococcus salivarius (DSM 20560) and Streptococcus sobrinus (DSM 20742) were rehydrated and grown anaerobically on caso, caso blood (containing 5% sheep blood) and caso chlorophyll (containing 5% spinach extract) agar for 3 days at 37°C, in the dark.					
35631205	9	0	dep	increasing	1123:1132	arg1	FL					1120:1121	FL	1120:1121	FL	1120:1121	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	9	0	dep	increasing	1123:1132	arg1	%					1136:1136	7%	1135:1136	7%	1135:1136	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	9	0	dep	increasing	1123:1132	arg1	'					1119:1119	'	1119:1119	'	1119:1119	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	9	0	dep	increasing	1123:1132	arg1	%					1146:1146	21%	1144:1146	21%	1144:1146	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	9	0	dep	increasing	1123:1132	arg1	%					1141:1141	13%	1139:1141	13%	1139:1141	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	5	1	theme	high	580:583	arg1	chromatography					604:617	high performance liquid chromatography	580:617	high performance liquid chromatography with fluorescence detection	580:645	Samples were analysed using high performance liquid chromatography with fluorescence detection.					
35631205	1	2	theme	Human	131:135	arg1	HMOs					160:163	HMOs	160:163	HMOs	160:163	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35631205	1	2	theme	Human	131:135	arg1	oligosaccharides					142:157	Human milk oligosaccharides	131:157	Human milk oligosaccharides (HMOs)	131:164	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35631205	4	3	theme	77	484:485	arg1	women					487:491	77 women	484:491	77 women with a gestational age of ranging from 14.3 to 40.9 weeks	484:549	(2) Methods: AF was collected during diagnostic amniocentesis, fetal surgery, or C-section from 77 women with a gestational age of ranging from 14.3 to 40.9 weeks.					
35631205	0	4	theme	Specific	67:74	arg1	Patterns					76:83	Specific Patterns	67:83	Specific Patterns Dependent on Gestational Age	67:112	Human Milk Oligosaccharides Are Present in Amniotic Fluid and Show Specific Patterns Dependent on Gestational Age.					
35631205	3	5	from	profiles	343:350	arg1	AF					355:356	AF	355:356	AF	355:356	We aimed to assess HMO profiles in AF over the course of gestation.					
35631205	2	6	from	blood	289:293	arg1	present					273:279	present	273:279	present	273:279	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	1	7	theme	milk	137:140	arg1	HMOs					160:163	HMOs	160:163	HMOs	160:163	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35631205	1	7	theme	milk	137:140	arg1	oligosaccharides					142:157	Human milk oligosaccharides	131:157	Human milk oligosaccharides (HMOs)	131:164	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35631205	5	8	theme	fluorescence	624:635	arg1	detection					637:645	fluorescence detection	624:645	fluorescence detection	624:645	Samples were analysed using high performance liquid chromatography with fluorescence detection.					
35631205	2	9	from	fluid	308:312	arg1	present					273:279	present	273:279	present	273:279	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	6	10	dep	Results	652:658	arg1	found					664:668	found	664:668	found	664:668	(3) Results: We found lactose and up to 16 HMO structures in all AF samples investigated, starting at 14 weeks of gestation.					
35631205	9	11	theme	late	1020:1023	arg1	pregnancy					1025:1033	early, mid- and late pregnancy	1004:1033	early, mid- and late pregnancy	1004:1033	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	9	12	theme	mid-	1011:1014	arg1	pregnancy					1025:1033	early, mid- and late pregnancy	1004:1033	early, mid- and late pregnancy	1004:1033	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	2	13	from	present	273:279	arg1	AF					315:316	AF	315:316	AF	315:316	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	2	13	from	present	273:279	arg1	blood					289:293	cord blood	284:293	cord blood	284:293	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	2	13	from	present	273:279	arg1	fluid					308:312	amniotic fluid	299:312	amniotic fluid (AF)	299:317	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	9	14	dep	decreasing	1087:1096	arg1	%					1106:1106	25%	1104:1106	25%	1104:1106	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	9	14	dep	decreasing	1087:1096	arg1	%					1111:1111	24%	1109:1111	24%	1109:1111	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	9	14	dep	decreasing	1087:1096	arg1	%					1101:1101	44%	1099:1101	44%	1099:1101	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	0	15	theme	Dependent	85:93	arg1	Patterns					76:83	Specific Patterns	67:83	Specific Patterns Dependent on Gestational Age	67:112	Human Milk Oligosaccharides Are Present in Amniotic Fluid and Show Specific Patterns Dependent on Gestational Age.					
35631205	0	16	from	Fluid	52:56	arg1	Present					32:38	Present	32:38	Present	32:38	Human Milk Oligosaccharides Are Present in Amniotic Fluid and Show Specific Patterns Dependent on Gestational Age.					
35631205	11	17	theme	broad	1308:1312	arg1	HMOs					1325:1328	HMOs	1325:1328	HMOs	1325:1328	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	11	17	theme	broad	1308:1312	arg1	variety					1314:1320	a broad variety	1306:1320	a broad variety of HMOs	1306:1328	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	11	18	theme	extensive	1270:1278	arg1	contact					1280:1286	extensive contact	1270:1286	extensive contact of the fetus with a broad variety of HMOs	1270:1328	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	10	19	attach	present	1218:1224	arg1	pregnancy					1241:1249	pregnancy	1241:1249	pregnancy	1241:1249	(4) Conclusion: Our study shows that HMOs are already present in AF early in pregnancy.					
35631205	10	19	attach	present	1218:1224	arg1	AF					1229:1230	AF	1229:1230	AF	1229:1230	(4) Conclusion: Our study shows that HMOs are already present in AF early in pregnancy.					
35631205	10	19	attach	present	1218:1224	arg2	HMOs					1201:1204	HMOs	1201:1204	HMOs	1201:1204	(4) Conclusion: Our study shows that HMOs are already present in AF early in pregnancy.					
35631205	11	20	theme	fetal	1360:1364	arg1	development					1373:1383	fetal tissue development	1360:1383	fetal tissue development	1360:1383	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	8	21	theme	total	879:883	arg1	concentrations					889:902	Individual and total HMO concentrations	864:902	concentrations	889:902	Individual and total HMO concentrations were significantly positively correlated with gestational age.					
35631205	11	22	with	contact	1280:1286	arg1	HMOs					1325:1328	HMOs	1325:1328	HMOs	1325:1328	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	11	22	with	contact	1280:1286	arg1	variety					1314:1320	a broad variety	1306:1320	a broad variety of HMOs	1306:1328	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	0	23	theme	Gestational	98:108	arg1	Age					110:112	Gestational Age	98:112	Gestational Age	98:112	Human Milk Oligosaccharides Are Present in Amniotic Fluid and Show Specific Patterns Dependent on Gestational Age.					
35631205	9	24	theme	relative	1041:1048	arg1	concentrations					1050:1063	relative concentrations	1041:1063	relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%)	1041:1147	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	0	25	attach	Present	32:38	arg1	Fluid					52:56	Amniotic Fluid	43:56	Amniotic Fluid	43:56	Human Milk Oligosaccharides Are Present in Amniotic Fluid and Show Specific Patterns Dependent on Gestational Age.					
35631205	0	25	attach	Present	32:38	arg2	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides Are Present in Amniotic Fluid and Show Specific Patterns Dependent on Gestational Age.					
35631205	10	26	dep	Conclusion	1168:1177	arg1	4					1165:1165	4	1165:1165	4	1165:1165	(4) Conclusion: Our study shows that HMOs are already present in AF early in pregnancy.					
35631205	10	26	dep	Conclusion	1168:1177	arg1	shows					1190:1194	shows	1190:1194	shows that HMOs are already present in AF early in pregnancy	1190:1249	(4) Conclusion: Our study shows that HMOs are already present in AF early in pregnancy.					
35631205	0	27	theme	Milk	6:9	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides Are Present in Amniotic Fluid and Show Specific Patterns Dependent on Gestational Age.					
35631205	0	28	from	Present	32:38	arg1	Fluid					52:56	Amniotic Fluid	43:56	Amniotic Fluid	43:56	Human Milk Oligosaccharides Are Present in Amniotic Fluid and Show Specific Patterns Dependent on Gestational Age.					
35631205	2	29	attach	present	273:279	arg1	AF					315:316	AF	315:316	AF	315:316	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	2	29	attach	present	273:279	arg2	HMOs					259:262	HMOs	259:262	HMOs	259:262	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	2	29	attach	present	273:279	arg1	blood					289:293	cord blood	284:293	cord blood	284:293	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	2	29	attach	present	273:279	arg1	fluid					308:312	amniotic fluid	299:312	amniotic fluid (AF)	299:317	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	3	30	theme	HMO	339:341	arg1	profiles					343:350	HMO profiles	339:350	HMO profiles in AF	339:356	We aimed to assess HMO profiles in AF over the course of gestation.					
35631205	0	31	theme	Human	0:4	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides Are Present in Amniotic Fluid and Show Specific Patterns Dependent on Gestational Age.					
35631205	2	32	located	present	273:279	arg1	AF					315:316	AF	315:316	AF	315:316	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	2	32	located	present	273:279	arg2	HMOs					259:262	HMOs	259:262	HMOs	259:262	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	2	32	located	present	273:279	arg1	blood					289:293	cord blood	284:293	cord blood	284:293	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	2	32	located	present	273:279	arg1	fluid					308:312	amniotic fluid	299:312	amniotic fluid (AF)	299:317	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	8	33	theme	HMO	885:887	arg1	concentrations					889:902	Individual and total HMO concentrations	864:902	concentrations	889:902	Individual and total HMO concentrations were significantly positively correlated with gestational age.					
35631205	9	34	theme	decreasing	1087:1096	arg1	SL					1070:1071	3'SL	1068:1071	3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%)	1068:1147	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	11	35	theme	HMOs	1325:1328	arg1	HMOs					1325:1328	HMOs	1325:1328	HMOs	1325:1328	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	11	35	theme	HMOs	1325:1328	arg1	variety					1314:1320	a broad variety	1306:1320	a broad variety of HMOs	1306:1328	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	4	36	dep	Methods	392:398	arg1	collected					408:416	collected	408:416	was collected during diagnostic amniocentesis, fetal surgery, or C-section from 77 women with a gestational age of ranging from 14.3 to 40.9 weeks	404:549	(2) Methods: AF was collected during diagnostic amniocentesis, fetal surgery, or C-section from 77 women with a gestational age of ranging from 14.3 to 40.9 weeks.					
35631205	5	37	theme	performance	585:595	arg1	chromatography					604:617	high performance liquid chromatography	580:617	high performance liquid chromatography with fluorescence detection	580:645	Samples were analysed using high performance liquid chromatography with fluorescence detection.					
35631205	1	38	dep	Background	119:128	arg1	1					116:116	1	116:116	1	116:116	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35631205	1	38	dep	Background	119:128	arg1	found					178:182	found	178:182	are already found in maternal circulation in early pregnancy, changing with gestational age	166:256	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35631205	6	39	theme	HMO	691:693	arg1	structures					695:704	up to 16 HMO structures	682:704	up to 16 HMO structures	682:704	(3) Results: We found lactose and up to 16 HMO structures in all AF samples investigated, starting at 14 weeks of gestation.					
35631205	5	40	with	chromatography	604:617	arg1	detection					637:645	fluorescence detection	624:645	fluorescence detection	624:645	Samples were analysed using high performance liquid chromatography with fluorescence detection.					
35631205	5	41	theme	liquid	597:602	arg1	chromatography					604:617	high performance liquid chromatography	580:617	high performance liquid chromatography with fluorescence detection	580:645	Samples were analysed using high performance liquid chromatography with fluorescence detection.					
35631205	10	42	from	present	1218:1224	arg1	pregnancy					1241:1249	pregnancy	1241:1249	pregnancy	1241:1249	(4) Conclusion: Our study shows that HMOs are already present in AF early in pregnancy.					
35631205	10	42	from	present	1218:1224	arg1	AF					1229:1230	AF	1229:1230	AF	1229:1230	(4) Conclusion: Our study shows that HMOs are already present in AF early in pregnancy.					
35631205	9	43	theme	increasing	1123:1132	arg1	SL					1070:1071	3'SL	1068:1071	3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%)	1068:1147	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	6	44	dep	16	688:689	arg1	to					685:686	to	685:686	to	685:686	(3) Results: We found lactose and up to 16 HMO structures in all AF samples investigated, starting at 14 weeks of gestation.					
35631205	9	45	theme	SL	1070:1071	arg1	concentrations					1050:1063	relative concentrations	1041:1063	relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%)	1041:1147	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	9	46	theme	HMO	967:969	arg1	composition					971:981	HMO composition	967:981	HMO composition	967:981	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	3	47	theme	gestation	377:385	arg1	course					367:372	the course	363:372	the course of gestation	363:385	We aimed to assess HMO profiles in AF over the course of gestation.					
35631205	4	48	theme	diagnostic	425:434	arg1	amniocentesis					436:448	diagnostic amniocentesis	425:448	diagnostic amniocentesis	425:448	(2) Methods: AF was collected during diagnostic amniocentesis, fetal surgery, or C-section from 77 women with a gestational age of ranging from 14.3 to 40.9 weeks.					
35631205	8	49	theme	gestational	950:960	arg1	age					962:964	gestational age	950:964	gestational age	950:964	Individual and total HMO concentrations were significantly positively correlated with gestational age.					
35631205	10	50	from	pregnancy	1241:1249	arg1	present					1218:1224	present	1218:1224	present	1218:1224	(4) Conclusion: Our study shows that HMOs are already present in AF early in pregnancy.					
35631205	10	51	located	present	1218:1224	arg1	pregnancy					1241:1249	pregnancy	1241:1249	pregnancy	1241:1249	(4) Conclusion: Our study shows that HMOs are already present in AF early in pregnancy.					
35631205	10	51	located	present	1218:1224	arg1	AF					1229:1230	AF	1229:1230	AF	1229:1230	(4) Conclusion: Our study shows that HMOs are already present in AF early in pregnancy.					
35631205	10	51	located	present	1218:1224	arg2	HMOs					1201:1204	HMOs	1201:1204	HMOs	1201:1204	(4) Conclusion: Our study shows that HMOs are already present in AF early in pregnancy.					
35631205	2	52	theme	amniotic	299:306	arg1	AF					315:316	AF	315:316	AF	315:316	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	2	52	theme	amniotic	299:306	arg1	fluid					308:312	amniotic fluid	299:312	amniotic fluid (AF)	299:317	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	0	53	theme	Amniotic	43:50	arg1	Fluid					52:56	Amniotic Fluid	43:56	Amniotic Fluid	43:56	Human Milk Oligosaccharides Are Present in Amniotic Fluid and Show Specific Patterns Dependent on Gestational Age.					
35631205	4	54	theme	fetal	451:455	arg1	surgery					457:463	fetal surgery	451:463	fetal surgery	451:463	(2) Methods: AF was collected during diagnostic amniocentesis, fetal surgery, or C-section from 77 women with a gestational age of ranging from 14.3 to 40.9 weeks.					
35631205	6	55	theme	AF	713:714	arg1	samples					716:722	all AF samples	709:722	all AF samples investigated	709:735	(3) Results: We found lactose and up to 16 HMO structures in all AF samples investigated, starting at 14 weeks of gestation.					
35631205	11	56	theme	fetus	1295:1299	arg1	contact					1280:1286	extensive contact	1270:1286	extensive contact of the fetus with a broad variety of HMOs	1270:1328	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	8	57	theme	Individual	864:873	arg1	concentrations					889:902	Individual and total HMO concentrations	864:902	concentrations	889:902	Individual and total HMO concentrations were significantly positively correlated with gestational age.					
35631205	7	58	theme	abundant	849:856	arg1	3'-sialyllactose					782:797	3'-sialyllactose	782:797	3'-sialyllactose (3'SL)	782:804	Overall, 3'-sialyllactose (3'SL) and 2'-fucosyllactose (2'FL) were the most abundant HMOs.					
35631205	7	58	theme	abundant	849:856	arg1	HMOs					858:861	the most abundant HMOs	840:861	the most abundant HMOs	840:861	Overall, 3'-sialyllactose (3'SL) and 2'-fucosyllactose (2'FL) were the most abundant HMOs.					
35631205	7	58	theme	abundant	849:856	arg1	2'-fucosyllactose					810:826	2'-fucosyllactose	810:826	2'-fucosyllactose (2'FL)	810:833	Overall, 3'-sialyllactose (3'SL) and 2'-fucosyllactose (2'FL) were the most abundant HMOs.					
35631205	11	59	theme	pregnancy	1411:1419	arg1	course					1401:1406	the time course	1392:1406	the time course of pregnancy	1392:1419	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	1	60	theme	gestational	242:252	arg1	age					254:256	gestational age	242:256	gestational age	242:256	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35631205	1	61	theme	maternal	187:194	arg1	circulation					196:206	maternal circulation	187:206	maternal circulation in early pregnancy, changing with gestational age	187:256	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35631205	1	62	from	circulation	196:206	arg1	pregnancy					217:225	early pregnancy	211:225	early pregnancy	211:225	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35631205	11	63	theme	time	1396:1399	arg1	course					1401:1406	the time course	1392:1406	the time course of pregnancy	1392:1419	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	4	64	with	women	487:491	arg1	age					512:514	a gestational age	498:514	a gestational age of ranging from 14.3 to 40.9 weeks	498:549	(2) Methods: AF was collected during diagnostic amniocentesis, fetal surgery, or C-section from 77 women with a gestational age of ranging from 14.3 to 40.9 weeks.					
35631205	2	65	theme	cord	284:287	arg1	blood					289:293	cord blood	284:293	cord blood	284:293	HMOs are also present in cord blood and amniotic fluid (AF).					
35631205	1	66	located	found	178:182	arg2	oligosaccharides					142:157	Human milk oligosaccharides	131:157	Human milk oligosaccharides (HMOs)	131:164	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35631205	1	66	located	found	178:182	arg1	circulation					196:206	maternal circulation	187:206	maternal circulation in early pregnancy, changing with gestational age	187:256	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35631205	1	66	located	found	178:182	arg2	HMOs					160:163	HMOs	160:163	HMOs	160:163	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35631205	11	67	theme	tissue	1366:1371	arg1	development					1373:1383	fetal tissue development	1360:1383	fetal tissue development	1360:1383	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	9	68	theme	early	1004:1008	arg1	pregnancy					1025:1033	early, mid- and late pregnancy	1004:1033	early, mid- and late pregnancy	1004:1033	HMO composition also changed between early, mid- and late pregnancy, with relative concentrations of 3'SL significantly decreasing (44%, 25%, 24%) and 2'FL increasing (7%, 13%, 21%), respectively.					
35631205	6	69	theme	gestation	762:770	arg1	weeks					753:757	14 weeks	750:757	14 weeks of gestation	750:770	(3) Results: We found lactose and up to 16 HMO structures in all AF samples investigated, starting at 14 weeks of gestation.					
35631205	10	70	from	AF	1229:1230	arg1	present					1218:1224	present	1218:1224	present	1218:1224	(4) Conclusion: Our study shows that HMOs are already present in AF early in pregnancy.					
35631205	4	71	dep	40.9	540:543	arg1	to					537:538	to	537:538	to	537:538	(2) Methods: AF was collected during diagnostic amniocentesis, fetal surgery, or C-section from 77 women with a gestational age of ranging from 14.3 to 40.9 weeks.					
35631205	4	72	theme	gestational	500:510	arg1	age					512:514	a gestational age	498:514	a gestational age of ranging from 14.3 to 40.9 weeks	498:549	(2) Methods: AF was collected during diagnostic amniocentesis, fetal surgery, or C-section from 77 women with a gestational age of ranging from 14.3 to 40.9 weeks.					
35631205	11	73	from	roles	1342:1346	arg1	development					1373:1383	fetal tissue development	1360:1383	fetal tissue development	1360:1383	This demonstrates extensive contact of the fetus with a broad variety of HMOs, suggesting roles for HMOs in fetal tissue development during the time course of pregnancy.					
35631205	1	74	theme	early	211:215	arg1	pregnancy					217:225	early pregnancy	211:225	early pregnancy	211:225	(1) Background: Human milk oligosaccharides (HMOs) are already found in maternal circulation in early pregnancy, changing with gestational age.					
35647651	4	0	theme	islet	781:785	arg1	tissue					787:792	islet tissue	781:792	islet tissue with a clear and integrated morphology	781:831	Meanwhile, CMOS prevented STZ plus HFSD-induced damage to islet tissue with a clear and integrated morphology and reduced the glucagon/insulin area ratio (by 97.9% for 300 mg per kg b.w. per d CMOS).					
35647651	10	1	theme	AKT/IRS/AMPK	1757:1768	arg1	pathway					1780:1786	the AKT/IRS/AMPK signaling pathway	1753:1786	the AKT/IRS/AMPK signaling pathway	1753:1786	Furthermore, CMOS regulated the AKT/IRS/AMPK signaling pathway and improved glucose metabolism in the liver.					
35647651	3	2	theme	fasting	529:535	arg1	L-1					571:573	7.1-8.2 mmol L-1	558:573	7.1-8.2 mmol L-1	558:573	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	3	2	theme	fasting	529:535	arg1	level					551:555	the fasting blood glucose level	525:555	the fasting blood glucose level (7.1-8.2 mmol L-1)	525:574	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	10	3	theme	improved	1792:1799	arg1	metabolism					1809:1818	improved glucose metabolism	1792:1818	improved glucose metabolism	1792:1818	Furthermore, CMOS regulated the AKT/IRS/AMPK signaling pathway and improved glucose metabolism in the liver.					
35647651	1	4	theme	glucose	218:224	arg1	metabolism					226:235	improved glucose metabolism	209:235	improved glucose metabolism	209:235	Functional oligosaccharides show anti-diabetic effects through inflammation regulation with improved glucose metabolism.					
35647651	9	5	theme	metabolism	1560:1569	arg1	disorder					1571:1578	glucose metabolism disorder	1552:1578	glucose metabolism disorder	1552:1578	Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation.					
35647651	7	6	theme	molecular	1280:1288	arg1	mechanisms					1290:1299	The molecular mechanisms	1276:1299	The molecular mechanisms involved in the anti-diabetic effects of CMOS	1276:1345	The molecular mechanisms involved in the anti-diabetic effects of CMOS were further studied.					
35647651	3	7	theme	test	682:685	arg1	curve					687:691	the oral glucose tolerance test curve	655:691	the oral glucose tolerance test curve	655:691	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	11	8	theme	functional	1921:1930	arg1	supplement					1940:1949	a functional dietary supplement	1919:1949	a functional dietary supplement	1919:1949	Findings obtained here implicated that CMOS could modulate metabolic-inflammation as a functional dietary supplement.					
35647651	11	8	theme	functional	1921:1930	arg1	metabolic-inflammation					1893:1914	metabolic-inflammation	1893:1914	metabolic-inflammation	1893:1914	Findings obtained here implicated that CMOS could modulate metabolic-inflammation as a functional dietary supplement.					
35647651	2	9	theme	novel	253:257	arg1	prebiotics					259:268	novel prebiotics	253:268	novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS)	253:322	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	3	10	theme	glucose	664:670	arg1	test					682:685	the oral glucose tolerance test	655:685	the oral glucose tolerance test curve	655:691	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	3	11	theme	glucose	543:549	arg1	L-1					571:573	7.1-8.2 mmol L-1	558:573	7.1-8.2 mmol L-1	558:573	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	3	11	theme	glucose	543:549	arg1	level					551:555	the fasting blood glucose level	525:555	the fasting blood glucose level (7.1-8.2 mmol L-1)	525:574	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	11	12	theme	dietary	1932:1938	arg1	supplement					1940:1949	a functional dietary supplement	1919:1949	a functional dietary supplement	1919:1949	Findings obtained here implicated that CMOS could modulate metabolic-inflammation as a functional dietary supplement.					
35647651	11	12	theme	dietary	1932:1938	arg1	metabolic-inflammation					1893:1914	metabolic-inflammation	1893:1914	metabolic-inflammation	1893:1914	Findings obtained here implicated that CMOS could modulate metabolic-inflammation as a functional dietary supplement.					
35647651	0	13	from	metabolism	88:97	arg1	rats					111:114	diabetic rats	102:114	diabetic rats	102:114	Manno-oligosaccharides from cassia seed gum ameliorate inflammation and improve glucose metabolism in diabetic rats.					
35647651	6	14	theme	increased	1190:1198	arg1	abundance					1209:1217	an increased relative abundance	1187:1217	an increased relative abundance of Bifidobacteria	1187:1235	Upon 16S rDNA sequencing, it was found that CMOS regulated the microbiota composition in the cecum with an increased relative abundance of Bifidobacteria, while that of Shigella was decreased.					
35647651	4	15	theme	clear	801:805	arg1	morphology					822:831	a clear and integrated morphology	799:831	a clear and integrated morphology	799:831	Meanwhile, CMOS prevented STZ plus HFSD-induced damage to islet tissue with a clear and integrated morphology and reduced the glucagon/insulin area ratio (by 97.9% for 300 mg per kg b.w. per d CMOS).					
35647651	3	16	theme	mmol	566:569	arg1	L-1					571:573	7.1-8.2 mmol L-1	558:573	7.1-8.2 mmol L-1	558:573	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	3	16	theme	mmol	566:569	arg1	level					551:555	the fasting blood glucose level	525:555	the fasting blood glucose level (7.1-8.2 mmol L-1)	525:574	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	4	17	theme	integrated	811:820	arg1	morphology					822:831	a clear and integrated morphology	799:831	a clear and integrated morphology	799:831	Meanwhile, CMOS prevented STZ plus HFSD-induced damage to islet tissue with a clear and integrated morphology and reduced the glucagon/insulin area ratio (by 97.9% for 300 mg per kg b.w. per d CMOS).					
35647651	6	18	theme	rDNA	1092:1095	arg1	sequencing					1097:1106	16S rDNA sequencing	1088:1106	16S rDNA sequencing	1088:1106	Upon 16S rDNA sequencing, it was found that CMOS regulated the microbiota composition in the cecum with an increased relative abundance of Bifidobacteria, while that of Shigella was decreased.					
35647651	2	19	theme	seed	308:311	arg1	CMOS					318:321	CMOS	318:321	CMOS	318:321	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	2	19	theme	seed	308:311	arg1	gum					313:315	cassia seed gum	301:315	cassia seed gum (CMOS)	301:322	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	9	20	theme	interleukin-1β	1624:1637	arg1	secretion					1639:1647	reduced interleukin-1β secretion	1616:1647	reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d)	1616:1705	Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation.					
35647651	5	21	theme	recovered	1004:1012	arg1	expression					1019:1028	recovered mRNA expression	1004:1028	recovered mRNA expression of Zo-1 and occludin to the normal comparable level	1004:1080	CMOS also reduced metabolic endotoxemia and maintained intestinal integrity with recovered mRNA expression of Zo-1 and occludin to the normal comparable level.					
35647651	0	22	theme	cassia	28:33	arg1	gum					40:42	cassia seed gum	28:42	cassia seed gum	28:42	Manno-oligosaccharides from cassia seed gum ameliorate inflammation and improve glucose metabolism in diabetic rats.					
35647651	1	23	theme	inflammation	180:191	arg1	regulation					193:202	inflammation regulation	180:202	inflammation regulation with improved glucose metabolism	180:235	Functional oligosaccharides show anti-diabetic effects through inflammation regulation with improved glucose metabolism.					
35647651	4	24	theme	area	866:869	arg1	ratio					871:875	the glucagon/insulin area ratio	845:875	the glucagon/insulin area ratio	845:875	Meanwhile, CMOS prevented STZ plus HFSD-induced damage to islet tissue with a clear and integrated morphology and reduced the glucagon/insulin area ratio (by 97.9% for 300 mg per kg b.w. per d CMOS).					
35647651	8	25	theme	mRNA	1386:1389	arg1	expression					1391:1400	the mRNA expression	1382:1400	the mRNA expression of Tlr2 and Tlr4 in the intestines of STZ plus HFSD-induced rats	1382:1465	CMOS reduced the mRNA expression of Tlr2 and Tlr4 in the intestines of STZ plus HFSD-induced rats.					
35647651	4	26	dep	reduced	837:843	arg1	%					885:885	97.9%	881:885	97.9% for 300 mg per kg b.w. per d CMOS	881:919	Meanwhile, CMOS prevented STZ plus HFSD-induced damage to islet tissue with a clear and integrated morphology and reduced the glucagon/insulin area ratio (by 97.9% for 300 mg per kg b.w. per d CMOS).					
35647651	9	27	from	activation	1509:1518	arg1	liver					1541:1545	liver	1541:1545	liver	1541:1545	Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation.					
35647651	9	27	from	activation	1509:1518	arg1	intestine					1527:1535	intestine	1527:1535	intestine	1527:1535	Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation.					
35647651	6	28	theme	Bifidobacteria	1222:1235	arg1	abundance					1209:1217	an increased relative abundance	1187:1217	an increased relative abundance of Bifidobacteria	1187:1235	Upon 16S rDNA sequencing, it was found that CMOS regulated the microbiota composition in the cecum with an increased relative abundance of Bifidobacteria, while that of Shigella was decreased.					
35647651	0	29	from	gum	40:42	arg1	Manno-oligosaccharides					0:21	Manno-oligosaccharides	0:21	Manno-oligosaccharides from cassia seed gum	0:42	Manno-oligosaccharides from cassia seed gum ameliorate inflammation and improve glucose metabolism in diabetic rats.					
35647651	3	30	theme	oral	659:662	arg1	test					682:685	the oral glucose tolerance test	655:685	the oral glucose tolerance test curve	655:691	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	9	31	theme	mg	1685:1686	arg1	CMOS					1668:1671	CMOS	1668:1671	CMOS of 300-1200 mg per kg b.w. per d	1668:1704	Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation.					
35647651	9	32	dep	intestine	1527:1535	arg1	the					1523:1525	the	1523:1525	the	1523:1525	Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation.					
35647651	5	33	theme	comparable	1065:1074	arg1	level					1076:1080	the normal comparable level	1054:1080	the normal comparable level	1054:1080	CMOS also reduced metabolic endotoxemia and maintained intestinal integrity with recovered mRNA expression of Zo-1 and occludin to the normal comparable level.					
35647651	1	34	theme	Functional	117:126	arg1	oligosaccharides					128:143	Functional oligosaccharides	117:143	Functional oligosaccharides	117:143	Functional oligosaccharides show anti-diabetic effects through inflammation regulation with improved glucose metabolism.					
35647651	2	35	from	gum	313:315	arg1	manno-oligosaccharides					273:294	manno-oligosaccharides	273:294	manno-oligosaccharides from cassia seed gum (CMOS)	273:322	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	2	35	from	gum	313:315	arg1	prebiotics					259:268	novel prebiotics	253:268	novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS)	253:322	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	2	36	dep	-induced	420:427	arg1	diet					409:412	high-fat and high-sugar diet	385:412	high-fat and high-sugar diet	385:412	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	2	36	dep	-induced	420:427	arg1	diet					351:354	the diet	347:354	the diet of streptozotocin (STZ)	347:378	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	2	37	theme	-induced	420:427	arg1	rats					429:432	the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats	347:432	the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats	347:432	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	5	38	theme	Zo-1	1033:1036	arg1	expression					1019:1028	recovered mRNA expression	1004:1028	recovered mRNA expression of Zo-1 and occludin to the normal comparable level	1004:1080	CMOS also reduced metabolic endotoxemia and maintained intestinal integrity with recovered mRNA expression of Zo-1 and occludin to the normal comparable level.					
35647651	8	39	theme	STZ	1440:1442	arg1	intestines					1426:1435	the intestines	1422:1435	the intestines of STZ plus HFSD-induced rats	1422:1465	CMOS reduced the mRNA expression of Tlr2 and Tlr4 in the intestines of STZ plus HFSD-induced rats.					
35647651	5	40	theme	occludin	1042:1049	arg1	expression					1019:1028	recovered mRNA expression	1004:1028	recovered mRNA expression of Zo-1 and occludin to the normal comparable level	1004:1080	CMOS also reduced metabolic endotoxemia and maintained intestinal integrity with recovered mRNA expression of Zo-1 and occludin to the normal comparable level.					
35647651	9	41	theme	Nlrp3	1479:1483	arg1	activation					1509:1518	Nlrp3 associated inflammasome activation	1479:1518	Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder	1479:1578	Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation.					
35647651	8	42	theme	HFSD-induced	1449:1460	arg1	rats					1462:1465	HFSD-induced rats	1449:1465	HFSD-induced rats	1449:1465	CMOS reduced the mRNA expression of Tlr2 and Tlr4 in the intestines of STZ plus HFSD-induced rats.					
35647651	2	43	theme	streptozotocin	359:372	arg1	diet					351:354	the diet	347:354	the diet of streptozotocin (STZ)	347:378	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	9	44	theme	inflammasome	1496:1507	arg1	activation					1509:1518	Nlrp3 associated inflammasome activation	1479:1518	Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder	1479:1578	Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation.					
35647651	9	45	with	activation	1509:1518	arg1	disorder					1571:1578	glucose metabolism disorder	1552:1578	glucose metabolism disorder	1552:1578	Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation.					
35647651	6	46	with	composition	1157:1167	arg1	abundance					1209:1217	an increased relative abundance	1187:1217	an increased relative abundance of Bifidobacteria	1187:1235	Upon 16S rDNA sequencing, it was found that CMOS regulated the microbiota composition in the cecum with an increased relative abundance of Bifidobacteria, while that of Shigella was decreased.					
35647651	3	47	theme	-24.5	715:719	arg1	%					714:714	%	714:714	%	714:714	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	10	48	theme	signaling	1770:1778	arg1	pathway					1780:1786	the AKT/IRS/AMPK signaling pathway	1753:1786	the AKT/IRS/AMPK signaling pathway	1753:1786	Furthermore, CMOS regulated the AKT/IRS/AMPK signaling pathway and improved glucose metabolism in the liver.					
35647651	6	49	theme	microbiota	1146:1155	arg1	composition					1157:1167	the microbiota composition	1142:1167	the microbiota composition in the cecum with an increased relative abundance of Bifidobacteria	1142:1235	Upon 16S rDNA sequencing, it was found that CMOS regulated the microbiota composition in the cecum with an increased relative abundance of Bifidobacteria, while that of Shigella was decreased.					
35647651	7	50	theme	CMOS	1342:1345	arg1	effects					1331:1337	the anti-diabetic effects	1313:1337	the anti-diabetic effects of CMOS	1313:1345	The molecular mechanisms involved in the anti-diabetic effects of CMOS were further studied.					
35647651	7	51	theme	anti-diabetic	1317:1329	arg1	effects					1331:1337	the anti-diabetic effects	1313:1337	the anti-diabetic effects of CMOS	1313:1345	The molecular mechanisms involved in the anti-diabetic effects of CMOS were further studied.					
35647651	10	52	theme	glucose	1801:1807	arg1	metabolism					1809:1818	improved glucose metabolism	1792:1818	improved glucose metabolism	1792:1818	Furthermore, CMOS regulated the AKT/IRS/AMPK signaling pathway and improved glucose metabolism in the liver.					
35647651	1	53	theme	improved	209:216	arg1	metabolism					226:235	improved glucose metabolism	209:235	improved glucose metabolism	209:235	Functional oligosaccharides show anti-diabetic effects through inflammation regulation with improved glucose metabolism.					
35647651	9	54	theme	glucose	1552:1558	arg1	metabolism					1560:1569	glucose metabolism	1552:1569	glucose metabolism disorder	1552:1578	Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation.					
35647651	4	55	theme	HFSD-induced	758:769	arg1	damage					771:776	HFSD-induced damage	758:776	HFSD-induced damage	758:776	Meanwhile, CMOS prevented STZ plus HFSD-induced damage to islet tissue with a clear and integrated morphology and reduced the glucagon/insulin area ratio (by 97.9% for 300 mg per kg b.w. per d CMOS).					
35647651	3	56	theme	blood	537:541	arg1	L-1					571:573	7.1-8.2 mmol L-1	558:573	7.1-8.2 mmol L-1	558:573	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	3	56	theme	blood	537:541	arg1	level					551:555	the fasting blood glucose level	525:555	the fasting blood glucose level (7.1-8.2 mmol L-1)	525:574	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	3	57	theme	tolerance	672:680	arg1	test					682:685	the oral glucose tolerance test	655:685	the oral glucose tolerance test curve	655:691	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	6	58	theme	relative	1200:1207	arg1	abundance					1209:1217	an increased relative abundance	1187:1217	an increased relative abundance of Bifidobacteria	1187:1235	Upon 16S rDNA sequencing, it was found that CMOS regulated the microbiota composition in the cecum with an increased relative abundance of Bifidobacteria, while that of Shigella was decreased.					
35647651	5	59	theme	metabolic	941:949	arg1	endotoxemia					951:961	metabolic endotoxemia	941:961	metabolic endotoxemia	941:961	CMOS also reduced metabolic endotoxemia and maintained intestinal integrity with recovered mRNA expression of Zo-1 and occludin to the normal comparable level.					
35647651	2	60	theme	manno-oligosaccharides	273:294	arg1	prebiotics					259:268	novel prebiotics	253:268	novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS)	253:322	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	3	61	theme	20.0	710:713	arg1	%					714:714	%	714:714	%	714:714	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	1	62	theme	anti-diabetic	150:162	arg1	effects					164:170	anti-diabetic effects	150:170	anti-diabetic effects	150:170	Functional oligosaccharides show anti-diabetic effects through inflammation regulation with improved glucose metabolism.					
35647651	4	63	with	tissue	787:792	arg1	morphology					822:831	a clear and integrated morphology	799:831	a clear and integrated morphology	799:831	Meanwhile, CMOS prevented STZ plus HFSD-induced damage to islet tissue with a clear and integrated morphology and reduced the glucagon/insulin area ratio (by 97.9% for 300 mg per kg b.w. per d CMOS).					
35647651	9	64	theme	reduced	1616:1622	arg1	secretion					1639:1647	reduced interleukin-1β secretion	1616:1647	reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d)	1616:1705	Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation.					
35647651	6	65	theme	16S	1088:1090	arg1	sequencing					1097:1106	16S rDNA sequencing	1088:1106	16S rDNA sequencing	1088:1106	Upon 16S rDNA sequencing, it was found that CMOS regulated the microbiota composition in the cecum with an increased relative abundance of Bifidobacteria, while that of Shigella was decreased.					
35647651	0	66	theme	glucose	80:86	arg1	metabolism					88:97	glucose metabolism	80:97	glucose metabolism in diabetic rats	80:114	Manno-oligosaccharides from cassia seed gum ameliorate inflammation and improve glucose metabolism in diabetic rats.					
35647651	2	67	theme	cassia	301:306	arg1	CMOS					318:321	CMOS	318:321	CMOS	318:321	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	2	67	theme	cassia	301:306	arg1	gum					313:315	cassia seed gum	301:315	cassia seed gum (CMOS)	301:322	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	0	68	theme	diabetic	102:109	arg1	rats					111:114	diabetic rats	102:114	diabetic rats	102:114	Manno-oligosaccharides from cassia seed gum ameliorate inflammation and improve glucose metabolism in diabetic rats.					
35647651	5	69	theme	mRNA	1014:1017	arg1	expression					1019:1028	recovered mRNA expression	1004:1028	recovered mRNA expression of Zo-1 and occludin to the normal comparable level	1004:1080	CMOS also reduced metabolic endotoxemia and maintained intestinal integrity with recovered mRNA expression of Zo-1 and occludin to the normal comparable level.					
35647651	1	70	with	regulation	193:202	arg1	metabolism					226:235	improved glucose metabolism	209:235	improved glucose metabolism	209:235	Functional oligosaccharides show anti-diabetic effects through inflammation regulation with improved glucose metabolism.					
35647651	4	71	theme	glucagon/insulin	849:864	arg1	ratio					871:875	the glucagon/insulin area ratio	845:875	the glucagon/insulin area ratio	845:875	Meanwhile, CMOS prevented STZ plus HFSD-induced damage to islet tissue with a clear and integrated morphology and reduced the glucagon/insulin area ratio (by 97.9% for 300 mg per kg b.w. per d CMOS).					
35647651	8	72	theme	Tlr2	1405:1408	arg1	expression					1391:1400	the mRNA expression	1382:1400	the mRNA expression of Tlr2 and Tlr4 in the intestines of STZ plus HFSD-induced rats	1382:1465	CMOS reduced the mRNA expression of Tlr2 and Tlr4 in the intestines of STZ plus HFSD-induced rats.					
35647651	2	73	from	prebiotics	259:268	arg1	CMOS					318:321	CMOS	318:321	CMOS	318:321	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	2	73	from	prebiotics	259:268	arg1	gum					313:315	cassia seed gum	301:315	cassia seed gum (CMOS)	301:322	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	8	74	theme	Tlr4	1414:1417	arg1	expression					1391:1400	the mRNA expression	1382:1400	the mRNA expression of Tlr2 and Tlr4 in the intestines of STZ plus HFSD-induced rats	1382:1465	CMOS reduced the mRNA expression of Tlr2 and Tlr4 in the intestines of STZ plus HFSD-induced rats.					
35647651	8	75	from	expression	1391:1400	arg1	intestines					1426:1435	the intestines	1422:1435	the intestines of STZ plus HFSD-induced rats	1422:1465	CMOS reduced the mRNA expression of Tlr2 and Tlr4 in the intestines of STZ plus HFSD-induced rats.					
35647651	5	76	theme	intestinal	978:987	arg1	integrity					989:997	intestinal integrity	978:997	intestinal integrity	978:997	CMOS also reduced metabolic endotoxemia and maintained intestinal integrity with recovered mRNA expression of Zo-1 and occludin to the normal comparable level.					
35647651	2	77	theme	high-sugar	398:407	arg1	diet					409:412	high-fat and high-sugar diet	385:412	high-fat and high-sugar diet	385:412	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	0	78	theme	seed	35:38	arg1	gum					40:42	cassia seed gum	28:42	cassia seed gum	28:42	Manno-oligosaccharides from cassia seed gum ameliorate inflammation and improve glucose metabolism in diabetic rats.					
35647651	5	79	theme	normal	1058:1063	arg1	level					1076:1080	the normal comparable level	1054:1080	the normal comparable level	1054:1080	CMOS also reduced metabolic endotoxemia and maintained intestinal integrity with recovered mRNA expression of Zo-1 and occludin to the normal comparable level.					
35647651	4	80	theme	d	914:914	arg1	CMOS					916:919	d CMOS	914:919	d CMOS	914:919	Meanwhile, CMOS prevented STZ plus HFSD-induced damage to islet tissue with a clear and integrated morphology and reduced the glucagon/insulin area ratio (by 97.9% for 300 mg per kg b.w. per d CMOS).					
35647651	3	81	theme	model	605:609	arg1	group					611:615	the model group	601:615	the model group (14.2 mmol L-1)	601:631	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	3	81	theme	model	605:609	arg1	L-1					628:630	14.2 mmol L-1	618:630	14.2 mmol L-1	618:630	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	8	82	theme	rats	1462:1465	arg1	intestines					1426:1435	the intestines	1422:1435	the intestines of STZ plus HFSD-induced rats	1422:1465	CMOS reduced the mRNA expression of Tlr2 and Tlr4 in the intestines of STZ plus HFSD-induced rats.					
35647651	3	83	theme	mmol	623:626	arg1	group					611:615	the model group	601:615	the model group (14.2 mmol L-1)	601:631	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	3	83	theme	mmol	623:626	arg1	L-1					628:630	14.2 mmol L-1	618:630	14.2 mmol L-1	618:630	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	9	84	theme	associated	1485:1494	arg1	activation					1509:1518	Nlrp3 associated inflammasome activation	1479:1518	Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder	1479:1578	Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation.					
35647651	3	85	dep	CMOS	462:465	arg1	mg					477:478	300-1200 mg	468:478	300-1200 mg per kg b.w. per d	468:496	After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%.					
35647651	2	86	theme	high-fat	385:392	arg1	diet					409:412	high-fat and high-sugar diet	385:412	high-fat and high-sugar diet	385:412	In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats.					
35647651	6	87	from	composition	1157:1167	arg1	cecum					1176:1180	the cecum	1172:1180	the cecum	1172:1180	Upon 16S rDNA sequencing, it was found that CMOS regulated the microbiota composition in the cecum with an increased relative abundance of Bifidobacteria, while that of Shigella was decreased.					
37400423	3	0	theme	-treated	478:485	arg1	mice					487:490	azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice	431:490	azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice	431:505	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	9	1	theme	CRC	1471:1473	arg1	strategy					1459:1466	a potential sex-targeting therapeutic strategy	1421:1466	a potential sex-targeting therapeutic strategy of CRC	1421:1473	Modulating sex-biased gut microbiota and metabolites could be a potential sex-targeting therapeutic strategy of CRC.					
37400423	0	2	theme	Male-Biased	0:10	arg1	Microbiome					16:25	Male-Biased Gut Microbiome	0:25	Male-Biased Gut Microbiome	0:25	Male-Biased Gut Microbiome and Metabolites Aggravate Colorectal Cancer Development.					
37400423	6	3	theme	metabolism	1182:1191	arg1	pathway					1193:1199	glycerophospholipids metabolism pathway	1161:1199	glycerophospholipids metabolism pathway	1161:1199	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	8	4	theme	sex-biased	1277:1286	arg1	microbiome					1292:1301	the sex-biased gut microbiome	1273:1301	the sex-biased gut microbiome	1273:1301	In conclusion, the sex-biased gut microbiome and metabolites contribute to sexual dimorphism in CRC.					
37400423	2	5	theme	microbiota	296:305	arg1	perspective					266:276	the perspective	262:276	the perspective of sex-biased gut microbiota and metabolites	262:321	This study aims to explain the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites.					
37400423	2	6	theme	sex-biased	281:290	arg1	microbiota					296:305	sex-biased gut microbiota	281:305	sex-biased gut microbiota	281:305	This study aims to explain the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites.					
37400423	4	7	theme	pseudo-germ	611:621	arg1	mice					623:626	pseudo-germ mice	611:626	pseudo-germ mice receiving fecal samples from male mice or patients	611:677	Moreover, pseudo-germ mice receiving fecal samples from male mice or patients show more severe intestinal barrier damage and higher level of inflammation.					
37400423	5	8	theme	increased	821:829	arg1	bacteria					842:849	increased pathogenic bacteria	821:849	increased pathogenic bacteria Akkermansia muciniphila	821:873	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	2	9	theme	potential	207:215	arg1	causes					217:222	the potential causes	203:222	the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites	203:321	This study aims to explain the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites.					
37400423	4	10	theme	severe	689:694	arg1	damage					715:720	intestinal barrier damage	696:720	intestinal barrier damage	696:720	Moreover, pseudo-germ mice receiving fecal samples from male mice or patients show more severe intestinal barrier damage and higher level of inflammation.					
37400423	1	11	theme	cancer	151:156	arg1	incidence					107:115	higher incidence and mortality rates	100:135	incidence	107:115	Men demonstrate higher incidence and mortality rates of colorectal cancer (CRC) than women.					
37400423	1	11	theme	cancer	151:156	arg1	rates					131:135	higher incidence and mortality rates	100:135	rates	131:135	Men demonstrate higher incidence and mortality rates of colorectal cancer (CRC) than women.					
37400423	1	12	theme	colorectal	140:149	arg1	CRC					159:161	CRC	159:161	CRC	159:161	Men demonstrate higher incidence and mortality rates of colorectal cancer (CRC) than women.					
37400423	1	12	theme	colorectal	140:149	arg1	cancer					151:156	colorectal cancer	140:156	colorectal cancer (CRC) than women	140:173	Men demonstrate higher incidence and mortality rates of colorectal cancer (CRC) than women.					
37400423	4	13	theme	male	657:660	arg1	mice					662:665	male mice	657:665	male mice	657:665	Moreover, pseudo-germ mice receiving fecal samples from male mice or patients show more severe intestinal barrier damage and higher level of inflammation.					
37400423	3	14	theme	gut	579:581	arg1	function					591:598	more impaired gut barrier function	565:598	more impaired gut barrier function	565:598	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	6	15	theme	gut	1014:1016	arg1	metabolites					1018:1028	Sex-biased gut metabolites	1003:1028	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice	1003:1101	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	6	16	from	metabolites	1018:1028	arg1	mice					1045:1048	pseudo-germ mice	1033:1048	pseudo-germ mice receiving fecal sample from CRC patients	1033:1089	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	6	16	from	metabolites	1018:1028	arg1	mice					1098:1101	CRC mice	1094:1101	CRC mice	1094:1101	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	7	17	theme	models	1250:1255	arg1	tumorigenesis					1223:1235	tumorigenesis	1223:1235	tumorigenesis of CRC mouse models	1223:1255	Sexual dimorphism in tumorigenesis of CRC mouse models.					
37400423	8	18	from	dimorphism	1340:1349	arg1	CRC					1354:1356	CRC	1354:1356	CRC	1354:1356	In conclusion, the sex-biased gut microbiome and metabolites contribute to sexual dimorphism in CRC.					
37400423	5	19	theme	Parabacteroides	897:911	arg1	goldsteinii					913:923	probiotic Parabacteroides goldsteinii	887:923	probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice	887:1000	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	3	20	theme	sexual	346:351	arg1	dimorphism					353:362	sexual dimorphism	346:362	sexual dimorphism in colorectal tumorigenesis	346:390	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	7	21	theme	CRC	1240:1242	arg1	models					1250:1255	CRC mouse models	1240:1255	CRC mouse models	1240:1255	Sexual dimorphism in tumorigenesis of CRC mouse models.					
37400423	5	22	theme	fecal	974:978	arg1	sample					980:985	fecal sample	974:985	fecal sample from male mice	974:1000	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	2	23	from	causes	217:222	arg1	CRC					253:255	CRC	253:255	CRC	253:255	This study aims to explain the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites.					
37400423	5	24	theme	male	933:936	arg1	mice					938:941	male mice	933:941	male mice	933:941	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	4	25	theme	inflammation	742:753	arg1	damage					715:720	intestinal barrier damage	696:720	intestinal barrier damage	696:720	Moreover, pseudo-germ mice receiving fecal samples from male mice or patients show more severe intestinal barrier damage and higher level of inflammation.					
37400423	4	25	theme	inflammation	742:753	arg1	level					733:737	higher level	726:737	higher level of inflammation	726:753	Moreover, pseudo-germ mice receiving fecal samples from male mice or patients show more severe intestinal barrier damage and higher level of inflammation.					
37400423	7	26	from	dimorphism	1209:1218	arg1	tumorigenesis					1223:1235	tumorigenesis	1223:1235	tumorigenesis of CRC mouse models	1223:1255	Sexual dimorphism in tumorigenesis of CRC mouse models.					
37400423	4	27	theme	barrier	707:713	arg1	damage					715:720	intestinal barrier damage	696:720	intestinal barrier damage	696:720	Moreover, pseudo-germ mice receiving fecal samples from male mice or patients show more severe intestinal barrier damage and higher level of inflammation.					
37400423	5	28	theme	gut	780:782	arg1	composition					795:805	gut microbiota composition	780:805	gut microbiota composition	780:805	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	9	29	theme	sex-biased	1370:1379	arg1	microbiota					1385:1394	sex-biased gut microbiota	1370:1394	sex-biased gut microbiota	1370:1394	Modulating sex-biased gut microbiota and metabolites could be a potential sex-targeting therapeutic strategy of CRC.					
37400423	5	30	from	goldsteinii	913:923	arg1	mice					938:941	male mice	933:941	male mice	933:941	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	5	30	from	goldsteinii	913:923	arg1	mice					959:962	pseudo-germ mice	947:962	pseudo-germ mice receiving fecal sample from male mice	947:1000	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	2	31	from	dimorphism	239:248	arg1	CRC					253:255	CRC	253:255	CRC	253:255	This study aims to explain the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites.					
37400423	2	32	theme	dimorphism	239:248	arg1	causes					217:222	the potential causes	203:222	the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites	203:321	This study aims to explain the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites.					
37400423	1	33	theme	mortality	121:129	arg1	rates					131:135	higher incidence and mortality rates	100:135	rates	131:135	Men demonstrate higher incidence and mortality rates of colorectal cancer (CRC) than women.					
37400423	2	34	from	CRC	253:255	arg1	causes					217:222	the potential causes	203:222	the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites	203:321	This study aims to explain the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites.					
37400423	6	35	theme	fecal	1060:1064	arg1	sample					1066:1071	fecal sample	1060:1071	fecal sample	1060:1071	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	5	36	dep	Akkermansia	851:861	arg1	muciniphila					863:873	muciniphila	863:873	muciniphila	863:873	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	0	37	theme	Colorectal	53:62	arg1	Development					71:81	Colorectal Cancer Development	53:81	Colorectal Cancer Development	53:81	Male-Biased Gut Microbiome and Metabolites Aggravate Colorectal Cancer Development.					
37400423	2	38	theme	sexual	232:237	arg1	dimorphism					239:248	such sexual dimorphism	227:248	such sexual dimorphism in CRC	227:255	This study aims to explain the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites.					
37400423	3	39	theme	sulfate	458:464	arg1	DSS					474:476	DSS	474:476	DSS	474:476	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	3	39	theme	sulfate	458:464	arg1	sodium					466:471	azoxymethane (AOM)/dextran sulfate sodium	431:471	azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice	431:505	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	9	40	theme	potential	1423:1431	arg1	strategy					1459:1466	a potential sex-targeting therapeutic strategy	1421:1466	a potential sex-targeting therapeutic strategy of CRC	1421:1473	Modulating sex-biased gut microbiota and metabolites could be a potential sex-targeting therapeutic strategy of CRC.					
37400423	6	41	theme	sex	1117:1119	arg1	dimorphism					1121:1130	sex dimorphism	1117:1130	sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway	1117:1199	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	9	42	theme	therapeutic	1447:1457	arg1	strategy					1459:1466	a potential sex-targeting therapeutic strategy	1421:1466	a potential sex-targeting therapeutic strategy of CRC	1421:1473	Modulating sex-biased gut microbiota and metabolites could be a potential sex-targeting therapeutic strategy of CRC.					
37400423	5	43	theme	significant	758:768	arg1	change					770:775	A significant change	756:775	A significant change in gut microbiota composition	756:805	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	6	44	theme	CRC	1094:1096	arg1	mice					1098:1101	CRC mice	1094:1101	CRC mice	1094:1101	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	3	45	with	mice	487:490	arg1	mice					502:505	male mice	497:505	male mice	497:505	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	5	46	theme	probiotic	887:895	arg1	goldsteinii					913:923	probiotic Parabacteroides goldsteinii	887:923	probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice	887:1000	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	3	47	theme	male	497:500	arg1	mice					502:505	male mice	497:505	male mice	497:505	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	5	48	theme	pathogenic	831:840	arg1	bacteria					842:849	increased pathogenic bacteria	821:849	increased pathogenic bacteria Akkermansia muciniphila	821:873	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	5	49	from	mice	997:1000	arg1	sample					980:985	fecal sample	974:985	fecal sample from male mice	974:1000	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	5	50	theme	Akkermansia	851:861	arg1	bacteria					842:849	increased pathogenic bacteria	821:849	increased pathogenic bacteria Akkermansia muciniphila	821:873	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	6	51	theme	glycerophospholipids	1161:1180	arg1	pathway					1193:1199	glycerophospholipids metabolism pathway	1161:1199	glycerophospholipids metabolism pathway	1161:1199	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	4	52	theme	fecal	638:642	arg1	samples					644:650	fecal samples	638:650	fecal samples	638:650	Moreover, pseudo-germ mice receiving fecal samples from male mice or patients show more severe intestinal barrier damage and higher level of inflammation.					
37400423	2	53	theme	gut	292:294	arg1	microbiota					296:305	sex-biased gut microbiota	281:305	sex-biased gut microbiota	281:305	This study aims to explain the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites.					
37400423	8	54	theme	gut	1288:1290	arg1	microbiome					1292:1301	the sex-biased gut microbiome	1273:1301	the sex-biased gut microbiome	1273:1301	In conclusion, the sex-biased gut microbiome and metabolites contribute to sexual dimorphism in CRC.					
37400423	2	55	theme	such	227:230	arg1	dimorphism					239:248	such sexual dimorphism	227:248	such sexual dimorphism in CRC	227:255	This study aims to explain the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites.					
37400423	3	56	from	dimorphism	353:362	arg1	tumorigenesis					378:390	colorectal tumorigenesis	367:390	colorectal tumorigenesis	367:390	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	3	57	theme	barrier	583:589	arg1	function					591:598	more impaired gut barrier function	565:598	more impaired gut barrier function	565:598	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	3	58	dep	have	507:510	arg1	accompanied					550:560	accompanied	550:560	accompanied by more impaired gut barrier function	550:598	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	6	59	theme	pseudo-germ	1033:1043	arg1	mice					1045:1048	pseudo-germ mice	1033:1048	pseudo-germ mice receiving fecal sample from CRC patients	1033:1089	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	8	60	theme	sexual	1333:1338	arg1	dimorphism					1340:1349	sexual dimorphism	1333:1349	sexual dimorphism in CRC	1333:1356	In conclusion, the sex-biased gut microbiome and metabolites contribute to sexual dimorphism in CRC.					
37400423	3	61	theme	impaired	570:577	arg1	function					591:598	more impaired gut barrier function	565:598	more impaired gut barrier function	565:598	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	2	62	from	perspective	266:276	arg1	causes					217:222	the potential causes	203:222	the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites	203:321	This study aims to explain the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites.					
37400423	7	63	theme	mouse	1244:1248	arg1	models					1250:1255	CRC mouse models	1240:1255	CRC mouse models	1240:1255	Sexual dimorphism in tumorigenesis of CRC mouse models.					
37400423	5	64	from	change	770:775	arg1	composition					795:805	gut microbiota composition	780:805	gut microbiota composition	780:805	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	3	65	dep	more	537:540	arg1	tumors					542:547	tumors	542:547	tumors	542:547	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	6	66	theme	Sex-biased	1003:1012	arg1	metabolites					1018:1028	Sex-biased gut metabolites	1003:1028	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice	1003:1101	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	4	67	theme	higher	726:731	arg1	level					733:737	higher level	726:737	higher level of inflammation	726:753	Moreover, pseudo-germ mice receiving fecal samples from male mice or patients show more severe intestinal barrier damage and higher level of inflammation.					
37400423	2	68	theme	metabolites	311:321	arg1	perspective					266:276	the perspective	262:276	the perspective of sex-biased gut microbiota and metabolites	262:321	This study aims to explain the potential causes of such sexual dimorphism in CRC from the perspective of sex-biased gut microbiota and metabolites.					
37400423	3	69	theme	azoxymethane	431:442	arg1	DSS					474:476	DSS	474:476	DSS	474:476	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	3	69	theme	azoxymethane	431:442	arg1	sodium					466:471	azoxymethane (AOM)/dextran sulfate sodium	431:471	azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice	431:505	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	0	70	theme	Gut	12:14	arg1	Microbiome					16:25	Male-Biased Gut Microbiome	0:25	Male-Biased Gut Microbiome	0:25	Male-Biased Gut Microbiome and Metabolites Aggravate Colorectal Cancer Development.					
37400423	1	71	theme	higher	100:105	arg1	incidence					107:115	higher incidence and mortality rates	100:135	incidence	107:115	Men demonstrate higher incidence and mortality rates of colorectal cancer (CRC) than women.					
37400423	6	72	theme	CRC	1078:1080	arg1	patients					1082:1089	CRC patients	1078:1089	CRC patients	1078:1089	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	4	73	theme	intestinal	696:705	arg1	damage					715:720	intestinal barrier damage	696:720	intestinal barrier damage	696:720	Moreover, pseudo-germ mice receiving fecal samples from male mice or patients show more severe intestinal barrier damage and higher level of inflammation.					
37400423	5	74	theme	microbiota	784:793	arg1	composition					795:805	gut microbiota composition	780:805	gut microbiota composition	780:805	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	9	75	theme	gut	1381:1383	arg1	microbiota					1385:1394	sex-biased gut microbiota	1370:1394	sex-biased gut microbiota	1370:1394	Modulating sex-biased gut microbiota and metabolites could be a potential sex-targeting therapeutic strategy of CRC.					
37400423	5	76	theme	pseudo-germ	947:957	arg1	mice					959:962	pseudo-germ mice	947:962	pseudo-germ mice receiving fecal sample from male mice	947:1000	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	3	77	theme	colorectal	367:376	arg1	tumorigenesis					378:390	colorectal tumorigenesis	367:390	colorectal tumorigenesis	367:390	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	3	78	theme	/dextran	449:456	arg1	DSS					474:476	DSS	474:476	DSS	474:476	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	3	78	theme	/dextran	449:456	arg1	sodium					466:471	azoxymethane (AOM)/dextran sulfate sodium	431:471	azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice	431:505	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	6	79	from	dimorphism	1121:1130	arg1	tumorigenesis					1139:1151	CRC tumorigenesis	1135:1151	CRC tumorigenesis	1135:1151	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	0	80	theme	Cancer	64:69	arg1	Development					71:81	Colorectal Cancer Development	53:81	Colorectal Cancer Development	53:81	Male-Biased Gut Microbiome and Metabolites Aggravate Colorectal Cancer Development.					
37400423	3	81	located	observed	395:402	arg2	dimorphism					353:362	sexual dimorphism	346:362	sexual dimorphism in colorectal tumorigenesis	346:390	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	3	81	located	observed	395:402	arg1	mice					422:425	mice	422:425	mice	422:425	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	3	81	located	observed	395:402	arg1	ApcMin/					412:418	ApcMin/	412:418	ApcMin/	412:418	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	6	82	theme	CRC	1135:1137	arg1	tumorigenesis					1139:1151	CRC tumorigenesis	1135:1151	CRC tumorigenesis	1135:1151	Sex-biased gut metabolites in pseudo-germ mice receiving fecal sample from CRC patients or CRC mice contribute to sex dimorphism in CRC tumorigenesis through glycerophospholipids metabolism pathway.					
37400423	5	83	theme	male	992:995	arg1	mice					997:1000	male mice	992:1000	male mice	992:1000	A significant change in gut microbiota composition is found with increased pathogenic bacteria Akkermansia muciniphila and deplets probiotic Parabacteroides goldsteinii in both male mice and pseudo-germ mice receiving fecal sample from male mice.					
37400423	3	84	theme	sodium	466:471	arg1	mice					487:490	azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice	431:490	azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice	431:505	The results show that sexual dimorphism in colorectal tumorigenesis is observed in both ApcMin/ + mice and azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice with male mice have significantly larger and more tumors, accompanied by more impaired gut barrier function.					
37400423	9	85	theme	sex-targeting	1433:1445	arg1	strategy					1459:1466	a potential sex-targeting therapeutic strategy	1421:1466	a potential sex-targeting therapeutic strategy of CRC	1421:1473	Modulating sex-biased gut microbiota and metabolites could be a potential sex-targeting therapeutic strategy of CRC.					
37400423	7	86	theme	Sexual	1202:1207	arg1	dimorphism					1209:1218	Sexual dimorphism	1202:1218	Sexual dimorphism in tumorigenesis of CRC mouse models	1202:1255	Sexual dimorphism in tumorigenesis of CRC mouse models.					
36710533	0	0	theme	microbiota	70:79	arg1	diversity					52:60	the diversity	48:60	the diversity of skin microbiota in healthy people	48:97	Sodium hyaluronates applied in the face affects the diversity of skin microbiota in healthy people.					
36710533	4	1	theme	hyaluronate	653:663	arg1	solution					665:672	sodium hyaluronate solution	646:672	sodium hyaluronate solution	646:672	METHODS Face of 20 healthy female volunteers between 18 and 24 years was smeared with sodium hyaluronate solution once per day.					
36710533	6	2	theme	pathogenic	924:933	arg1	Cutibacterium					953:965	Cutibacterium	953:965	Cutibacterium	953:965	RESULTS Facial application of HA can reduce the abundance of pathogenic bacteria, such as Cutibacterium and S. aureus, and increase the colonization of beneficial-bacteria.					
36710533	6	2	theme	pathogenic	924:933	arg1	aureus					974:979	S. aureus	971:979	S. aureus	971:979	RESULTS Facial application of HA can reduce the abundance of pathogenic bacteria, such as Cutibacterium and S. aureus, and increase the colonization of beneficial-bacteria.					
36710533	6	2	theme	pathogenic	924:933	arg1	bacteria					935:942	pathogenic bacteria	924:942	pathogenic bacteria	924:942	RESULTS Facial application of HA can reduce the abundance of pathogenic bacteria, such as Cutibacterium and S. aureus, and increase the colonization of beneficial-bacteria.					
36710533	0	3	theme	skin	65:68	arg1	microbiota					70:79	skin microbiota	65:79	skin microbiota	65:79	Sodium hyaluronates applied in the face affects the diversity of skin microbiota in healthy people.					
36710533	1	4	theme	many	146:149	arg1	effects					162:168	many beneficial effects	146:168	many beneficial effects	146:168	OBJECTIVE A healthy and stable microbiome has many beneficial effects on the host, while an unbalanced or disordered microbiome can lead to various skin diseases.					
36710533	0	5	theme	healthy	84:90	arg1	people					92:97	healthy people	84:97	healthy people	84:97	Sodium hyaluronates applied in the face affects the diversity of skin microbiota in healthy people.					
36710533	5	6	from	changes	819:825	arg1	composition					850:860	bacterial community composition	830:860	bacterial community composition	830:860	Cotton swabs were used to retrieve samples on days 0, 14, and 28, high-throughput sequencing of 16S rRNA was used to determine the changes in bacterial community composition.					
36710533	2	7	theme	Hyaluronic	263:272	arg1	acid					274:277	Hyaluronic acid	263:277	Hyaluronic acid	263:277	Hyaluronic acid is widely used in the cosmetics and pharmaceutical industries; however, specific reports on its effect on the skin microflora of healthy people have not been published.					
36710533	5	8	used	used	797:800	arg2	sequencing					770:779	high-throughput sequencing	754:779	high-throughput sequencing of 16S rRNA	754:791	Cotton swabs were used to retrieve samples on days 0, 14, and 28, high-throughput sequencing of 16S rRNA was used to determine the changes in bacterial community composition.					
36710533	3	9	theme	hyaluronate	499:509	arg1	effect					482:487	the effect	478:487	the effect of sodium hyaluronate on the facial microflora of healthy individuals	478:557	This study aimed to determine the effect of sodium hyaluronate on the facial microflora of healthy individuals.					
36710533	1	10	theme	various	240:246	arg1	diseases					253:260	various skin diseases	240:260	various skin diseases	240:260	OBJECTIVE A healthy and stable microbiome has many beneficial effects on the host, while an unbalanced or disordered microbiome can lead to various skin diseases.					
36710533	4	11	theme	sodium	646:651	arg1	solution					665:672	sodium hyaluronate solution	646:672	sodium hyaluronate solution	646:672	METHODS Face of 20 healthy female volunteers between 18 and 24 years was smeared with sodium hyaluronate solution once per day.					
36710533	1	12	contain	has	142:144	arg1	microbiome					131:140	A healthy and stable microbiome	110:140	OBJECTIVE A healthy and stable microbiome	100:140	OBJECTIVE A healthy and stable microbiome has many beneficial effects on the host, while an unbalanced or disordered microbiome can lead to various skin diseases.					
36710533	1	12	contain	has	142:144	arg2	effects					162:168	many beneficial effects	146:168	many beneficial effects	146:168	OBJECTIVE A healthy and stable microbiome has many beneficial effects on the host, while an unbalanced or disordered microbiome can lead to various skin diseases.					
36710533	8	13	theme	sodium	1180:1185	arg1	hyaluronate					1187:1197	sodium hyaluronate	1180:1197	sodium hyaluronate	1180:1197	Accordingly, sodium hyaluronate was found to have a positive effect on facial skin health.					
36710533	5	14	theme	high-throughput	754:768	arg1	sequencing					770:779	high-throughput sequencing	754:779	high-throughput sequencing of 16S rRNA	754:791	Cotton swabs were used to retrieve samples on days 0, 14, and 28, high-throughput sequencing of 16S rRNA was used to determine the changes in bacterial community composition.					
36710533	5	15	theme	community	840:848	arg1	composition					850:860	bacterial community composition	830:860	bacterial community composition	830:860	Cotton swabs were used to retrieve samples on days 0, 14, and 28, high-throughput sequencing of 16S rRNA was used to determine the changes in bacterial community composition.					
36710533	1	16	theme	beneficial	151:160	arg1	effects					162:168	many beneficial effects	146:168	many beneficial effects	146:168	OBJECTIVE A healthy and stable microbiome has many beneficial effects on the host, while an unbalanced or disordered microbiome can lead to various skin diseases.					
36710533	7	17	theme	acid	1122:1125	arg1	effect					1101:1106	the effect	1097:1106	the effect of hyaluronic acid on facial microflora in healthy people	1097:1164	CONCLUSION This is the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people.					
36710533	7	18	from	effect	1101:1106	arg1	microflora					1137:1146	facial microflora	1130:1146	facial microflora in healthy people	1130:1164	CONCLUSION This is the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people.					
36710533	5	19	theme	rRNA	788:791	arg1	sequencing					770:779	high-throughput sequencing	754:779	high-throughput sequencing of 16S rRNA	754:791	Cotton swabs were used to retrieve samples on days 0, 14, and 28, high-throughput sequencing of 16S rRNA was used to determine the changes in bacterial community composition.					
36710533	2	20	theme	healthy	408:414	arg1	people					416:421	healthy people	408:421	healthy people	408:421	Hyaluronic acid is widely used in the cosmetics and pharmaceutical industries; however, specific reports on its effect on the skin microflora of healthy people have not been published.					
36710533	4	21	with	smeared	633:639	arg1	solution					665:672	sodium hyaluronate solution	646:672	sodium hyaluronate solution	646:672	METHODS Face of 20 healthy female volunteers between 18 and 24 years was smeared with sodium hyaluronate solution once per day.					
36710533	3	22	theme	facial	518:523	arg1	microflora					525:534	the facial microflora	514:534	the facial microflora of healthy individuals	514:557	This study aimed to determine the effect of sodium hyaluronate on the facial microflora of healthy individuals.					
36710533	2	23	theme	specific	351:358	arg1	reports					360:366	specific reports	351:366	specific reports on its effect on the skin microflora of healthy people	351:421	Hyaluronic acid is widely used in the cosmetics and pharmaceutical industries; however, specific reports on its effect on the skin microflora of healthy people have not been published.					
36710533	0	24	theme	Sodium	0:5	arg1	hyaluronates					7:18	Sodium hyaluronates	0:18	Sodium hyaluronates applied in the face	0:38	Sodium hyaluronates applied in the face affects the diversity of skin microbiota in healthy people.					
36710533	1	25	theme	skin	248:251	arg1	diseases					253:260	various skin diseases	240:260	various skin diseases	240:260	OBJECTIVE A healthy and stable microbiome has many beneficial effects on the host, while an unbalanced or disordered microbiome can lead to various skin diseases.					
36710533	5	26	used	used	706:709	arg2	swabs					695:699	Cotton swabs	688:699	Cotton swabs	688:699	Cotton swabs were used to retrieve samples on days 0, 14, and 28, high-throughput sequencing of 16S rRNA was used to determine the changes in bacterial community composition.					
36710533	5	27	dep	retrieve	714:721	arg1	used					797:800	used	797:800	was used to determine the changes in bacterial community composition	793:860	Cotton swabs were used to retrieve samples on days 0, 14, and 28, high-throughput sequencing of 16S rRNA was used to determine the changes in bacterial community composition.					
36710533	8	28	theme	skin	1245:1248	arg1	health					1250:1255	facial skin health	1238:1255	facial skin health	1238:1255	Accordingly, sodium hyaluronate was found to have a positive effect on facial skin health.					
36710533	4	29	theme	female	587:592	arg1	volunteers					594:603	20 healthy female volunteers	576:603	20 healthy female volunteers	576:603	METHODS Face of 20 healthy female volunteers between 18 and 24 years was smeared with sodium hyaluronate solution once per day.					
36710533	2	30	theme	skin	389:392	arg1	microflora					394:403	the skin microflora	385:403	the skin microflora of healthy people	385:421	Hyaluronic acid is widely used in the cosmetics and pharmaceutical industries; however, specific reports on its effect on the skin microflora of healthy people have not been published.					
36710533	6	31	theme	HA	893:894	arg1	application					878:888	RESULTS Facial application	863:888	RESULTS Facial application of HA	863:894	RESULTS Facial application of HA can reduce the abundance of pathogenic bacteria, such as Cutibacterium and S. aureus, and increase the colonization of beneficial-bacteria.					
36710533	2	32	theme	pharmaceutical	315:328	arg1	industries					330:339	pharmaceutical industries	315:339	pharmaceutical industries	315:339	Hyaluronic acid is widely used in the cosmetics and pharmaceutical industries; however, specific reports on its effect on the skin microflora of healthy people have not been published.					
36710533	4	33	theme	healthy	579:585	arg1	volunteers					594:603	20 healthy female volunteers	576:603	20 healthy female volunteers	576:603	METHODS Face of 20 healthy female volunteers between 18 and 24 years was smeared with sodium hyaluronate solution once per day.					
36710533	3	34	theme	individuals	547:557	arg1	microflora					525:534	the facial microflora	514:534	the facial microflora of healthy individuals	514:557	This study aimed to determine the effect of sodium hyaluronate on the facial microflora of healthy individuals.					
36710533	5	35	theme	16S	784:786	arg1	rRNA					788:791	16S rRNA	784:791	16S rRNA	784:791	Cotton swabs were used to retrieve samples on days 0, 14, and 28, high-throughput sequencing of 16S rRNA was used to determine the changes in bacterial community composition.					
36710533	4	36	theme	volunteers	594:603	arg1	Face					568:571	METHODS Face	560:571	METHODS Face of 20 healthy female volunteers between 18 and 24 years	560:627	METHODS Face of 20 healthy female volunteers between 18 and 24 years was smeared with sodium hyaluronate solution once per day.					
36710533	7	37	theme	first	1059:1063	arg1	report					1075:1080	the first intuitive report	1055:1080	the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people	1055:1164	CONCLUSION This is the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people.					
36710533	7	37	theme	first	1059:1063	arg1	CONCLUSION					1036:1045	CONCLUSION	1036:1045	CONCLUSION This	1036:1050	CONCLUSION This is the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people.					
36710533	6	38	theme	Facial	871:876	arg1	application					878:888	RESULTS Facial application	863:888	RESULTS Facial application of HA	863:894	RESULTS Facial application of HA can reduce the abundance of pathogenic bacteria, such as Cutibacterium and S. aureus, and increase the colonization of beneficial-bacteria.					
36710533	0	39	from	diversity	52:60	arg1	people					92:97	healthy people	84:97	healthy people	84:97	Sodium hyaluronates applied in the face affects the diversity of skin microbiota in healthy people.					
36710533	1	40	theme	OBJECTIVE	100:108	arg1	microbiome					131:140	A healthy and stable microbiome	110:140	OBJECTIVE A healthy and stable microbiome	100:140	OBJECTIVE A healthy and stable microbiome has many beneficial effects on the host, while an unbalanced or disordered microbiome can lead to various skin diseases.					
36710533	2	41	theme	people	416:421	arg1	microflora					394:403	the skin microflora	385:403	the skin microflora of healthy people	385:421	Hyaluronic acid is widely used in the cosmetics and pharmaceutical industries; however, specific reports on its effect on the skin microflora of healthy people have not been published.					
36710533	6	42	theme	RESULTS	863:869	arg1	application					878:888	RESULTS Facial application	863:888	RESULTS Facial application of HA	863:894	RESULTS Facial application of HA can reduce the abundance of pathogenic bacteria, such as Cutibacterium and S. aureus, and increase the colonization of beneficial-bacteria.					
36710533	7	43	from	microflora	1137:1146	arg1	people					1159:1164	healthy people	1151:1164	healthy people	1151:1164	CONCLUSION This is the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people.					
36710533	3	44	theme	healthy	539:545	arg1	individuals					547:557	healthy individuals	539:557	healthy individuals	539:557	This study aimed to determine the effect of sodium hyaluronate on the facial microflora of healthy individuals.					
36710533	7	45	theme	facial	1130:1135	arg1	microflora					1137:1146	facial microflora	1130:1146	facial microflora in healthy people	1130:1164	CONCLUSION This is the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people.					
36710533	5	46	theme	bacterial	830:838	arg1	composition					850:860	bacterial community composition	830:860	bacterial community composition	830:860	Cotton swabs were used to retrieve samples on days 0, 14, and 28, high-throughput sequencing of 16S rRNA was used to determine the changes in bacterial community composition.					
36710533	8	47	theme	facial	1238:1243	arg1	health					1250:1255	facial skin health	1238:1255	facial skin health	1238:1255	Accordingly, sodium hyaluronate was found to have a positive effect on facial skin health.					
36710533	8	48	contain	have	1212:1215	arg2	effect					1228:1233	a positive effect	1217:1233	a positive effect	1217:1233	Accordingly, sodium hyaluronate was found to have a positive effect on facial skin health.					
36710533	8	48	contain	have	1212:1215	arg1	hyaluronate					1187:1197	sodium hyaluronate	1180:1197	sodium hyaluronate	1180:1197	Accordingly, sodium hyaluronate was found to have a positive effect on facial skin health.					
36710533	7	49	theme	intuitive	1065:1073	arg1	report					1075:1080	the first intuitive report	1055:1080	the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people	1055:1164	CONCLUSION This is the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people.					
36710533	7	49	theme	intuitive	1065:1073	arg1	CONCLUSION					1036:1045	CONCLUSION	1036:1045	CONCLUSION This	1036:1050	CONCLUSION This is the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people.					
36710533	2	50	from	reports	360:366	arg1	effect					375:380	its effect	371:380	its effect on the skin microflora of healthy people	371:421	Hyaluronic acid is widely used in the cosmetics and pharmaceutical industries; however, specific reports on its effect on the skin microflora of healthy people have not been published.					
36710533	1	51	theme	healthy	112:118	arg1	microbiome					131:140	A healthy and stable microbiome	110:140	OBJECTIVE A healthy and stable microbiome	100:140	OBJECTIVE A healthy and stable microbiome has many beneficial effects on the host, while an unbalanced or disordered microbiome can lead to various skin diseases.					
36710533	1	52	theme	unbalanced	192:201	arg1	microbiome					217:226	an unbalanced or disordered microbiome	189:226	an unbalanced or disordered microbiome	189:226	OBJECTIVE A healthy and stable microbiome has many beneficial effects on the host, while an unbalanced or disordered microbiome can lead to various skin diseases.					
36710533	7	53	dep	CONCLUSION	1036:1045	arg1	This					1047:1050	This	1047:1050	This	1047:1050	CONCLUSION This is the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people.					
36710533	3	54	theme	sodium	492:497	arg1	hyaluronate					499:509	sodium hyaluronate	492:509	sodium hyaluronate	492:509	This study aimed to determine the effect of sodium hyaluronate on the facial microflora of healthy individuals.					
36710533	2	55	from	effect	375:380	arg1	microflora					394:403	the skin microflora	385:403	the skin microflora of healthy people	385:421	Hyaluronic acid is widely used in the cosmetics and pharmaceutical industries; however, specific reports on its effect on the skin microflora of healthy people have not been published.					
36710533	8	56	theme	positive	1219:1226	arg1	effect					1228:1233	a positive effect	1217:1233	a positive effect	1217:1233	Accordingly, sodium hyaluronate was found to have a positive effect on facial skin health.					
36710533	4	57	theme	METHODS	560:566	arg1	Face					568:571	METHODS Face	560:571	METHODS Face of 20 healthy female volunteers between 18 and 24 years	560:627	METHODS Face of 20 healthy female volunteers between 18 and 24 years was smeared with sodium hyaluronate solution once per day.					
36710533	7	58	theme	healthy	1151:1157	arg1	people					1159:1164	healthy people	1151:1164	healthy people	1151:1164	CONCLUSION This is the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people.					
36710533	1	59	theme	stable	124:129	arg1	microbiome					131:140	A healthy and stable microbiome	110:140	OBJECTIVE A healthy and stable microbiome	100:140	OBJECTIVE A healthy and stable microbiome has many beneficial effects on the host, while an unbalanced or disordered microbiome can lead to various skin diseases.					
36710533	2	60	used	used	289:292	arg2	acid					274:277	Hyaluronic acid	263:277	Hyaluronic acid	263:277	Hyaluronic acid is widely used in the cosmetics and pharmaceutical industries; however, specific reports on its effect on the skin microflora of healthy people have not been published.					
36710533	1	61	theme	disordered	206:215	arg1	microbiome					217:226	an unbalanced or disordered microbiome	189:226	an unbalanced or disordered microbiome	189:226	OBJECTIVE A healthy and stable microbiome has many beneficial effects on the host, while an unbalanced or disordered microbiome can lead to various skin diseases.					
36710533	7	62	theme	hyaluronic	1111:1120	arg1	acid					1122:1125	hyaluronic acid	1111:1125	hyaluronic acid	1111:1125	CONCLUSION This is the first intuitive report to demonstrate the effect of hyaluronic acid on facial microflora in healthy people.					
36710533	6	63	theme	bacteria	935:942	arg1	abundance					911:919	the abundance	907:919	the abundance of pathogenic bacteria, such as Cutibacterium and S. aureus,	907:980	RESULTS Facial application of HA can reduce the abundance of pathogenic bacteria, such as Cutibacterium and S. aureus, and increase the colonization of beneficial-bacteria.					
36710533	6	64	theme	beneficial-bacteria	1015:1033	arg1	colonization					999:1010	the colonization	995:1010	the colonization of beneficial-bacteria	995:1033	RESULTS Facial application of HA can reduce the abundance of pathogenic bacteria, such as Cutibacterium and S. aureus, and increase the colonization of beneficial-bacteria.					
36710533	3	65	from	effect	482:487	arg1	microflora					525:534	the facial microflora	514:534	the facial microflora of healthy individuals	514:557	This study aimed to determine the effect of sodium hyaluronate on the facial microflora of healthy individuals.					
36710533	5	66	theme	Cotton	688:693	arg1	swabs					695:699	Cotton swabs	688:699	Cotton swabs	688:699	Cotton swabs were used to retrieve samples on days 0, 14, and 28, high-throughput sequencing of 16S rRNA was used to determine the changes in bacterial community composition.					
35221063	8	0	theme	estimated	1779:1787	arg1	eaten					1794:1798	estimated feed eaten	1779:1798	estimated feed eaten	1779:1798	There were no differences in estimated feed eaten, or pasture herbage accumulation, composition, or quality.					
35221063	4	1	dep	lactation	913:921	arg1	either					896:901	either	896:901	either	896:901	One herd was milked TAD for the whole lactation (August 2019 to May 2020), with the remaining 3 milked 3-in-2 for either the whole lactation, after December 1 when cows were an average of 101 d in milk, or after March 1 when days in milk averaged 189 d. Milking intervals over 48 h were 10-14-10-14 h for TAD and 12-18-18 h for 3-in-2.					
35221063	2	2	theme	pasture-based	519:531	arg1	farmers					539:545	pasture-based dairy farmers	519:545	pasture-based dairy farmers	519:545	The objective of this study was to quantify the farm system interactions associated with milking 3-in-2 at 3 stages of lactation, with the aim of providing guidance to pasture-based dairy farmers and advisors on the likely consequences of adopting 3-in-2 milking on farm productivity and business performance.					
35221063	3	3	theme	multiparous	674:684	arg1	Seventy-nine					661:672	Seventy-nine multiparous	661:684	Seventy-nine multiparous	661:684	Seventy-nine multiparous and 37 primiparous cows were randomly allocated to 4 experimental farms stocked at 3.5 cows/ha.					
35221063	5	4	theme	metric	1266:1271	arg1	value					1241:1245	the annualized value	1226:1245	the annualized value of the performance metric of interest	1226:1283	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	11	5	from	distances	2148:2156	arg1	potential					2183:2191	the research farm and potential	2161:2191	potential	2183:2191	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings suggests better production may be achieved with 3-in-2 milking on a commercial farm.					
35221063	11	5	from	distances	2148:2156	arg1	farm					2174:2177	the research farm and potential	2161:2191	farm	2174:2177	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings suggests better production may be achieved with 3-in-2 milking on a commercial farm.					
35221063	8	6	theme	herbage	1812:1818	arg1	accumulation					1820:1831	pasture herbage accumulation	1804:1831	pasture herbage accumulation	1804:1831	There were no differences in estimated feed eaten, or pasture herbage accumulation, composition, or quality.					
35221063	6	7	from	effect	1441:1446	arg1	yield					1498:1502	milk (-11%), protein (-8%), and lactose (-12%) yield	1451:1502	milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year	1451:1519	For the proportion of the season milked 3-in-2, there was a significant effect on milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year, but no effect of fat.					
35221063	7	8	theme	liveweight	1659:1668	arg1	change					1670:1675	liveweight change	1659:1675	liveweight change (+26%)	1659:1682	Additionally, there was a positive effect (+6%) on body condition score before dry-off and the energy required for liveweight change (+26%), and a negative effect on the energy required for walking (-30%).					
35221063	7	8	theme	liveweight	1659:1668	arg1	%					1681:1681	+26%	1678:1681	+26%	1678:1681	Additionally, there was a positive effect (+6%) on body condition score before dry-off and the energy required for liveweight change (+26%), and a negative effect on the energy required for walking (-30%).					
35221063	6	9	theme	protein	1464:1470	arg1	yield					1498:1502	milk (-11%), protein (-8%), and lactose (-12%) yield	1451:1502	milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year	1451:1519	For the proportion of the season milked 3-in-2, there was a significant effect on milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year, but no effect of fat.					
35221063	11	10	theme	commercial	2329:2338	arg1	farm					2340:2343	a commercial farm	2327:2343	a commercial farm	2327:2343	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings suggests better production may be achieved with 3-in-2 milking on a commercial farm.					
35221063	6	11	theme	milked	1402:1407	arg1	3-in-2					1409:1414	the season milked 3-in-2	1391:1414	the season milked 3-in-2	1391:1414	For the proportion of the season milked 3-in-2, there was a significant effect on milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year, but no effect of fat.					
35221063	2	12	theme	business	639:646	arg1	performance					648:658	business performance	639:658	business performance	639:658	The objective of this study was to quantify the farm system interactions associated with milking 3-in-2 at 3 stages of lactation, with the aim of providing guidance to pasture-based dairy farmers and advisors on the likely consequences of adopting 3-in-2 milking on farm productivity and business performance.					
35221063	11	13	theme	better	2270:2275	arg1	production					2277:2286	better production	2270:2286	better production	2270:2286	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings suggests better production may be achieved with 3-in-2 milking on a commercial farm.					
35221063	10	14	theme	commercial	1951:1960	arg1	farms					1962:1966	commercial farms	1951:1966	commercial farms	1951:1966	On commercial farms, the degree to which reduced milk income can be offset by lower costs will be highly farm-specific, but opportunities for savings were identified in the results.					
35221063	5	15	theme	Animal	1118:1123	arg1	pasture					1126:1132	Animal, pasture, and farm system data	1118:1154	pasture	1126:1132	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	0	16	theme	dairy	130:134	arg1	system					136:141	a pasture-based dairy system	114:141	a pasture-based dairy system	114:141	Reducing milking frequency from twice each day to three times each two days affected protein but not fat yield in a pasture-based dairy system.					
35221063	11	17	theme	short	2134:2138	arg1	distances					2148:2156	The short walking distances	2130:2156	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings	2130:2259	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings suggests better production may be achieved with 3-in-2 milking on a commercial farm.					
35221063	3	18	theme	experimental	739:750	arg1	farms					752:756	4 experimental farms	737:756	4 experimental farms stocked at 3.5 cows/ha	737:779	Seventy-nine multiparous and 37 primiparous cows were randomly allocated to 4 experimental farms stocked at 3.5 cows/ha.					
35221063	5	19	from	lactation	1316:1324	arg1	number					1294:1299	the number	1290:1299	the number of days in the lactation	1290:1324	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	5	20	theme	days	1304:1307	arg1	number					1294:1299	the number	1290:1299	the number of days in the lactation	1290:1324	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	6	21	theme	significant	1429:1439	arg1	effect					1441:1446	a significant effect	1427:1446	a significant effect on milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year	1427:1519	For the proportion of the season milked 3-in-2, there was a significant effect on milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year, but no effect of fat.					
35221063	10	22	theme	milk	1997:2000	arg1	income					2002:2007	reduced milk income	1989:2007	reduced milk income	1989:2007	On commercial farms, the degree to which reduced milk income can be offset by lower costs will be highly farm-specific, but opportunities for savings were identified in the results.					
35221063	0	23	from	day	43:45	arg1	frequency					17:25	Reducing milking frequency	0:25	Reducing milking frequency from twice each day to three times each two days	0:74	Reducing milking frequency from twice each day to three times each two days affected protein but not fat yield in a pasture-based dairy system.					
35221063	5	24	theme	interest	1276:1283	arg1	metric					1266:1271	the performance metric	1250:1271	the performance metric of interest	1250:1283	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	2	25	theme	likely	567:572	arg1	consequences					574:585	the likely consequences	563:585	the likely consequences of adopting 3-in-2 milking on farm productivity and business performance	563:658	The objective of this study was to quantify the farm system interactions associated with milking 3-in-2 at 3 stages of lactation, with the aim of providing guidance to pasture-based dairy farmers and advisors on the likely consequences of adopting 3-in-2 milking on farm productivity and business performance.					
35221063	7	26	theme	condition	1600:1608	arg1	score					1610:1614	body condition score	1595:1614	body condition score before dry-off and the energy required for liveweight change (+26%)	1595:1682	Additionally, there was a positive effect (+6%) on body condition score before dry-off and the energy required for liveweight change (+26%), and a negative effect on the energy required for walking (-30%).					
35221063	10	27	theme	lower	2026:2030	arg1	costs					2032:2036	lower costs	2026:2036	lower costs	2026:2036	On commercial farms, the degree to which reduced milk income can be offset by lower costs will be highly farm-specific, but opportunities for savings were identified in the results.					
35221063	9	28	theme	feed	1893:1896	arg1	allocation					1898:1907	feed allocation	1893:1907	feed allocation	1893:1907	Therefore, pasture management and feed allocation under 3-in-2 should be similar to TAD.					
35221063	0	29	theme	Reducing	0:7	arg1	frequency					17:25	Reducing milking frequency	0:25	Reducing milking frequency from twice each day to three times each two days	0:74	Reducing milking frequency from twice each day to three times each two days affected protein but not fat yield in a pasture-based dairy system.					
35221063	1	30	theme	dairy	216:220	arg1	workplace					222:230	the dairy workplace	212:230	the dairy workplace	212:230	Milking 3 times in 2 d (3-in-2) could enhance the attractiveness of the dairy workplace relative to twice-a-day milking (TAD) by reducing labor requirements for milking and increasing workforce flexibility.					
35221063	6	31	theme	milk	1451:1454	arg1	yield					1498:1502	milk (-11%), protein (-8%), and lactose (-12%) yield	1451:1502	milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year	1451:1519	For the proportion of the season milked 3-in-2, there was a significant effect on milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year, but no effect of fat.					
35221063	2	32	theme	3-in-2	599:604	arg1	milking					606:612	3-in-2 milking	599:612	3-in-2 milking	599:612	The objective of this study was to quantify the farm system interactions associated with milking 3-in-2 at 3 stages of lactation, with the aim of providing guidance to pasture-based dairy farmers and advisors on the likely consequences of adopting 3-in-2 milking on farm productivity and business performance.					
35221063	4	33	theme	whole	907:911	arg1	lactation					913:921	either the whole lactation	896:921	lactation	913:921	One herd was milked TAD for the whole lactation (August 2019 to May 2020), with the remaining 3 milked 3-in-2 for either the whole lactation, after December 1 when cows were an average of 101 d in milk, or after March 1 when days in milk averaged 189 d. Milking intervals over 48 h were 10-14-10-14 h for TAD and 12-18-18 h for 3-in-2.					
35221063	5	34	theme	independent	1347:1357	arg1	3-in-2					1333:1338	3-in-2	1333:1338	3-in-2	1333:1338	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	5	34	theme	independent	1347:1357	arg1	variable					1359:1366	the independent variable	1343:1366	the independent variable	1343:1366	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	5	35	theme	system	1144:1149	arg1	data					1151:1154	Animal, pasture, and farm system data	1118:1154	data	1151:1154	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	1	36	theme	twice-a-day	244:254	arg1	milking					256:262	twice-a-day milking	244:262	twice-a-day milking (TAD)	244:268	Milking 3 times in 2 d (3-in-2) could enhance the attractiveness of the dairy workplace relative to twice-a-day milking (TAD) by reducing labor requirements for milking and increasing workforce flexibility.					
35221063	1	36	theme	twice-a-day	244:254	arg1	TAD					265:267	TAD	265:267	TAD	265:267	Milking 3 times in 2 d (3-in-2) could enhance the attractiveness of the dairy workplace relative to twice-a-day milking (TAD) by reducing labor requirements for milking and increasing workforce flexibility.					
35221063	2	37	theme	farm	399:402	arg1	interactions					411:422	the farm system interactions	395:422	the farm system interactions associated with milking 3-in-2 at 3 stages of lactation, with the aim of providing guidance to pasture-based dairy farmers and advisors on the likely consequences of adopting 3-in-2 milking on farm productivity and business performance	395:658	The objective of this study was to quantify the farm system interactions associated with milking 3-in-2 at 3 stages of lactation, with the aim of providing guidance to pasture-based dairy farmers and advisors on the likely consequences of adopting 3-in-2 milking on farm productivity and business performance.					
35221063	11	38	theme	fewer	2246:2250	arg1	milkings					2252:2259	fewer milkings	2246:2259	fewer milkings	2246:2259	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings suggests better production may be achieved with 3-in-2 milking on a commercial farm.					
35221063	5	39	theme	dependent	1201:1209	arg1	variable					1211:1218	the dependent variable	1197:1218	the dependent variable being the annualized value of the performance metric of interest	1197:1283	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	7	40	theme	positive	1570:1577	arg1	effect					1579:1584	a positive effect	1568:1584	a positive effect (+6%) on body condition score before dry-off and the energy required for liveweight change (+26%)	1568:1682	Additionally, there was a positive effect (+6%) on body condition score before dry-off and the energy required for liveweight change (+26%), and a negative effect on the energy required for walking (-30%).					
35221063	7	40	theme	positive	1570:1577	arg1	%					1589:1589	+6%	1587:1589	+6%	1587:1589	Additionally, there was a positive effect (+6%) on body condition score before dry-off and the energy required for liveweight change (+26%), and a negative effect on the energy required for walking (-30%).					
35221063	7	41	from	effect	1700:1705	arg1	energy					1714:1719	the energy	1710:1719	the energy required for walking (-30%)	1710:1747	Additionally, there was a positive effect (+6%) on body condition score before dry-off and the energy required for liveweight change (+26%), and a negative effect on the energy required for walking (-30%).					
35221063	7	41	from	effect	1700:1705	arg1	score					1610:1614	body condition score	1595:1614	body condition score before dry-off and the energy required for liveweight change (+26%)	1595:1682	Additionally, there was a positive effect (+6%) on body condition score before dry-off and the energy required for liveweight change (+26%), and a negative effect on the energy required for walking (-30%).					
35221063	0	42	from	yield	105:109	arg1	system					136:141	a pasture-based dairy system	114:141	a pasture-based dairy system	114:141	Reducing milking frequency from twice each day to three times each two days affected protein but not fat yield in a pasture-based dairy system.					
35221063	6	43	theme	fat	1539:1541	arg1	effect					1529:1534	no effect	1526:1534	no effect of fat	1526:1541	For the proportion of the season milked 3-in-2, there was a significant effect on milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year, but no effect of fat.					
35221063	7	44	theme	negative	1691:1698	arg1	effect					1700:1705	a negative effect	1689:1705	a negative effect on the energy required for walking (-30%)	1689:1747	Additionally, there was a positive effect (+6%) on body condition score before dry-off and the energy required for liveweight change (+26%), and a negative effect on the energy required for walking (-30%).					
35221063	2	45	theme	dairy	533:537	arg1	farmers					539:545	pasture-based dairy farmers	519:545	pasture-based dairy farmers	519:545	The objective of this study was to quantify the farm system interactions associated with milking 3-in-2 at 3 stages of lactation, with the aim of providing guidance to pasture-based dairy farmers and advisors on the likely consequences of adopting 3-in-2 milking on farm productivity and business performance.					
35221063	5	46	theme	performance	1254:1264	arg1	metric					1266:1271	the performance metric	1250:1271	the performance metric of interest	1250:1283	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	4	47	theme	whole	814:818	arg1	lactation					820:828	the whole lactation	810:828	the whole lactation	810:828	One herd was milked TAD for the whole lactation (August 2019 to May 2020), with the remaining 3 milked 3-in-2 for either the whole lactation, after December 1 when cows were an average of 101 d in milk, or after March 1 when days in milk averaged 189 d. Milking intervals over 48 h were 10-14-10-14 h for TAD and 12-18-18 h for 3-in-2.					
35221063	8	48	theme	feed	1789:1792	arg1	eaten					1794:1798	estimated feed eaten	1779:1798	estimated feed eaten	1779:1798	There were no differences in estimated feed eaten, or pasture herbage accumulation, composition, or quality.					
35221063	4	49	from	days	1007:1010	arg1	milk					1015:1018	milk	1015:1018	milk	1015:1018	One herd was milked TAD for the whole lactation (August 2019 to May 2020), with the remaining 3 milked 3-in-2 for either the whole lactation, after December 1 when cows were an average of 101 d in milk, or after March 1 when days in milk averaged 189 d. Milking intervals over 48 h were 10-14-10-14 h for TAD and 12-18-18 h for 3-in-2.					
35221063	11	50	from	milkings	2252:2259	arg1	saved					2235:2239	the time saved	2226:2239	the time saved from fewer milkings	2226:2259	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings suggests better production may be achieved with 3-in-2 milking on a commercial farm.					
35221063	5	51	theme	annualized	1230:1239	arg1	value					1241:1245	the annualized value	1226:1245	the annualized value of the performance metric of interest	1226:1283	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	4	52	theme	milked	795:800	arg1	TAD					802:804	milked TAD	795:804	milked TAD for the whole lactation	795:828	One herd was milked TAD for the whole lactation (August 2019 to May 2020), with the remaining 3 milked 3-in-2 for either the whole lactation, after December 1 when cows were an average of 101 d in milk, or after March 1 when days in milk averaged 189 d. Milking intervals over 48 h were 10-14-10-14 h for TAD and 12-18-18 h for 3-in-2.					
35221063	8	53	from	differences	1764:1774	arg1	composition					1834:1844	composition	1834:1844	composition	1834:1844	There were no differences in estimated feed eaten, or pasture herbage accumulation, composition, or quality.					
35221063	8	53	from	differences	1764:1774	arg1	quality					1850:1856	quality	1850:1856	quality	1850:1856	There were no differences in estimated feed eaten, or pasture herbage accumulation, composition, or quality.					
35221063	8	53	from	differences	1764:1774	arg1	accumulation					1820:1831	pasture herbage accumulation	1804:1831	pasture herbage accumulation	1804:1831	There were no differences in estimated feed eaten, or pasture herbage accumulation, composition, or quality.					
35221063	8	53	from	differences	1764:1774	arg1	eaten					1794:1798	estimated feed eaten	1779:1798	estimated feed eaten	1779:1798	There were no differences in estimated feed eaten, or pasture herbage accumulation, composition, or quality.					
35221063	8	54	theme	pasture	1804:1810	arg1	accumulation					1820:1831	pasture herbage accumulation	1804:1831	pasture herbage accumulation	1804:1831	There were no differences in estimated feed eaten, or pasture herbage accumulation, composition, or quality.					
35221063	0	55	theme	fat	101:103	arg1	yield					105:109	fat yield	101:109	protein but not fat yield in a pasture-based dairy system	85:141	Reducing milking frequency from twice each day to three times each two days affected protein but not fat yield in a pasture-based dairy system.					
35221063	3	56	theme	primiparous	693:703	arg1	cows					705:708	37 primiparous cows	690:708	37 primiparous cows	690:708	Seventy-nine multiparous and 37 primiparous cows were randomly allocated to 4 experimental farms stocked at 3.5 cows/ha.					
35221063	1	57	theme	workforce	328:336	arg1	flexibility					338:348	workforce flexibility	328:348	workforce flexibility	328:348	Milking 3 times in 2 d (3-in-2) could enhance the attractiveness of the dairy workplace relative to twice-a-day milking (TAD) by reducing labor requirements for milking and increasing workforce flexibility.					
35221063	5	58	from	days	1304:1307	arg1	lactation					1316:1324	the lactation	1312:1324	the lactation	1312:1324	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	2	59	theme	lactation	470:478	arg1	stages					460:465	3 stages	458:465	3 stages of lactation	458:478	The objective of this study was to quantify the farm system interactions associated with milking 3-in-2 at 3 stages of lactation, with the aim of providing guidance to pasture-based dairy farmers and advisors on the likely consequences of adopting 3-in-2 milking on farm productivity and business performance.					
35221063	4	60	theme	Milking	1036:1042	arg1	intervals					1044:1052	189 d. Milking intervals	1029:1052	189 d. Milking intervals	1029:1052	One herd was milked TAD for the whole lactation (August 2019 to May 2020), with the remaining 3 milked 3-in-2 for either the whole lactation, after December 1 when cows were an average of 101 d in milk, or after March 1 when days in milk averaged 189 d. Milking intervals over 48 h were 10-14-10-14 h for TAD and 12-18-18 h for 3-in-2.					
35221063	0	61	theme	pasture-based	116:128	arg1	system					136:141	a pasture-based dairy system	114:141	a pasture-based dairy system	114:141	Reducing milking frequency from twice each day to three times each two days affected protein but not fat yield in a pasture-based dairy system.					
35221063	6	62	theme	season	1395:1400	arg1	3-in-2					1409:1414	the season milked 3-in-2	1391:1414	the season milked 3-in-2	1391:1414	For the proportion of the season milked 3-in-2, there was a significant effect on milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year, but no effect of fat.					
35221063	5	63	from	number	1294:1299	arg1	lactation					1316:1324	the lactation	1312:1324	the lactation	1312:1324	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	11	64	theme	walking	2140:2146	arg1	distances					2148:2156	The short walking distances	2130:2156	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings	2130:2259	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings suggests better production may be achieved with 3-in-2 milking on a commercial farm.					
35221063	1	65	from	times	154:158	arg1	3-in-2					168:173	3-in-2	168:173	3-in-2	168:173	Milking 3 times in 2 d (3-in-2) could enhance the attractiveness of the dairy workplace relative to twice-a-day milking (TAD) by reducing labor requirements for milking and increasing workforce flexibility.					
35221063	1	65	from	times	154:158	arg1	d					165:165	2 d	163:165	2 d (3-in-2)	163:174	Milking 3 times in 2 d (3-in-2) could enhance the attractiveness of the dairy workplace relative to twice-a-day milking (TAD) by reducing labor requirements for milking and increasing workforce flexibility.					
35221063	4	66	from	average	959:965	arg1	milk					979:982	milk	979:982	milk	979:982	One herd was milked TAD for the whole lactation (August 2019 to May 2020), with the remaining 3 milked 3-in-2 for either the whole lactation, after December 1 when cows were an average of 101 d in milk, or after March 1 when days in milk averaged 189 d. Milking intervals over 48 h were 10-14-10-14 h for TAD and 12-18-18 h for 3-in-2.					
35221063	7	67	from	effect	1579:1584	arg1	energy					1714:1719	the energy	1710:1719	the energy required for walking (-30%)	1710:1747	Additionally, there was a positive effect (+6%) on body condition score before dry-off and the energy required for liveweight change (+26%), and a negative effect on the energy required for walking (-30%).					
35221063	7	67	from	effect	1579:1584	arg1	score					1610:1614	body condition score	1595:1614	body condition score before dry-off and the energy required for liveweight change (+26%)	1595:1682	Additionally, there was a positive effect (+6%) on body condition score before dry-off and the energy required for liveweight change (+26%), and a negative effect on the energy required for walking (-30%).					
35221063	6	68	theme	lactose	1483:1489	arg1	yield					1498:1502	milk (-11%), protein (-8%), and lactose (-12%) yield	1451:1502	milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year	1451:1519	For the proportion of the season milked 3-in-2, there was a significant effect on milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year, but no effect of fat.					
35221063	1	69	theme	labor	282:286	arg1	requirements					288:299	labor requirements	282:299	labor requirements for milking	282:311	Milking 3 times in 2 d (3-in-2) could enhance the attractiveness of the dairy workplace relative to twice-a-day milking (TAD) by reducing labor requirements for milking and increasing workforce flexibility.					
35221063	9	70	theme	pasture	1870:1876	arg1	management					1878:1887	pasture management	1870:1887	pasture management	1870:1887	Therefore, pasture management and feed allocation under 3-in-2 should be similar to TAD.					
35221063	7	71	theme	body	1595:1598	arg1	score					1610:1614	body condition score	1595:1614	body condition score before dry-off and the energy required for liveweight change (+26%)	1595:1682	Additionally, there was a positive effect (+6%) on body condition score before dry-off and the energy required for liveweight change (+26%), and a negative effect on the energy required for walking (-30%).					
35221063	10	72	theme	reduced	1989:1995	arg1	income					2002:2007	reduced milk income	1989:2007	reduced milk income	1989:2007	On commercial farms, the degree to which reduced milk income can be offset by lower costs will be highly farm-specific, but opportunities for savings were identified in the results.					
35221063	2	73	theme	farm	617:620	arg1	productivity					622:633	farm productivity	617:633	farm productivity	617:633	The objective of this study was to quantify the farm system interactions associated with milking 3-in-2 at 3 stages of lactation, with the aim of providing guidance to pasture-based dairy farmers and advisors on the likely consequences of adopting 3-in-2 milking on farm productivity and business performance.					
35221063	0	74	theme	milking	9:15	arg1	frequency					17:25	Reducing milking frequency	0:25	Reducing milking frequency from twice each day to three times each two days	0:74	Reducing milking frequency from twice each day to three times each two days affected protein but not fat yield in a pasture-based dairy system.					
35221063	1	75	theme	workplace	222:230	arg1	attractiveness					194:207	the attractiveness	190:207	the attractiveness of the dairy workplace relative to twice-a-day milking (TAD)	190:268	Milking 3 times in 2 d (3-in-2) could enhance the attractiveness of the dairy workplace relative to twice-a-day milking (TAD) by reducing labor requirements for milking and increasing workforce flexibility.					
35221063	6	76	theme	3-in-2	1409:1414	arg1	proportion					1377:1386	the proportion	1373:1386	the proportion of the season milked 3-in-2	1373:1414	For the proportion of the season milked 3-in-2, there was a significant effect on milk (-11%), protein (-8%), and lactose (-12%) yield per cow per year, but no effect of fat.					
35221063	0	77	dep	times	56:60	arg1	days					71:74	each two days	62:74	three times each two days	50:74	Reducing milking frequency from twice each day to three times each two days affected protein but not fat yield in a pasture-based dairy system.					
35221063	2	78	theme	study	373:377	arg1	objective					355:363	The objective	351:363	The objective of this study	351:377	The objective of this study was to quantify the farm system interactions associated with milking 3-in-2 at 3 stages of lactation, with the aim of providing guidance to pasture-based dairy farmers and advisors on the likely consequences of adopting 3-in-2 milking on farm productivity and business performance.					
35221063	11	79	theme	farm	2204:2207	arg1	management					2209:2218	farm management	2204:2218	farm management using the time saved from fewer milkings	2204:2259	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings suggests better production may be achieved with 3-in-2 milking on a commercial farm.					
35221063	5	80	theme	farm	1139:1142	arg1	data					1151:1154	Animal, pasture, and farm system data	1118:1154	data	1151:1154	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	2	81	theme	system	404:409	arg1	interactions					411:422	the farm system interactions	395:422	the farm system interactions associated with milking 3-in-2 at 3 stages of lactation, with the aim of providing guidance to pasture-based dairy farmers and advisors on the likely consequences of adopting 3-in-2 milking on farm productivity and business performance	395:658	The objective of this study was to quantify the farm system interactions associated with milking 3-in-2 at 3 stages of lactation, with the aim of providing guidance to pasture-based dairy farmers and advisors on the likely consequences of adopting 3-in-2 milking on farm productivity and business performance.					
35221063	11	82	dep	farm	2174:2177	arg1	improve					2196:2202	improve	2196:2202	to improve farm management using the time saved from fewer milkings	2193:2259	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings suggests better production may be achieved with 3-in-2 milking on a commercial farm.					
35221063	11	83	theme	time	2230:2233	arg1	saved					2235:2239	the time saved	2226:2239	the time saved from fewer milkings	2226:2259	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings suggests better production may be achieved with 3-in-2 milking on a commercial farm.					
35221063	11	84	theme	research	2165:2172	arg1	farm					2174:2177	the research farm and potential	2161:2191	farm	2174:2177	The short walking distances on the research farm and potential to improve farm management using the time saved from fewer milkings suggests better production may be achieved with 3-in-2 milking on a commercial farm.					
35221063	5	85	theme	linear	1173:1178	arg1	regression					1180:1189	linear regression	1173:1189	linear regression	1173:1189	Animal, pasture, and farm system data were analyzed by linear regression, with the dependent variable being the annualized value of the performance metric of interest, and the number of days in the lactation milked 3-in-2 as the independent variable.					
35221063	4	86	theme	d	974:974	arg1	cows					946:949	cows	946:949	cows	946:949	One herd was milked TAD for the whole lactation (August 2019 to May 2020), with the remaining 3 milked 3-in-2 for either the whole lactation, after December 1 when cows were an average of 101 d in milk, or after March 1 when days in milk averaged 189 d. Milking intervals over 48 h were 10-14-10-14 h for TAD and 12-18-18 h for 3-in-2.					
35221063	4	86	theme	d	974:974	arg1	average					959:965	an average	956:965	an average of 101 d	956:974	One herd was milked TAD for the whole lactation (August 2019 to May 2020), with the remaining 3 milked 3-in-2 for either the whole lactation, after December 1 when cows were an average of 101 d in milk, or after March 1 when days in milk averaged 189 d. Milking intervals over 48 h were 10-14-10-14 h for TAD and 12-18-18 h for 3-in-2.					
35221063	4	87	dep	TAD	802:804	arg1	with					857:860	with	857:860	with	857:860	One herd was milked TAD for the whole lactation (August 2019 to May 2020), with the remaining 3 milked 3-in-2 for either the whole lactation, after December 1 when cows were an average of 101 d in milk, or after March 1 when days in milk averaged 189 d. Milking intervals over 48 h were 10-14-10-14 h for TAD and 12-18-18 h for 3-in-2.					
35221063	4	87	dep	TAD	802:804	arg1	August					831:836	August	831:836	August	831:836	One herd was milked TAD for the whole lactation (August 2019 to May 2020), with the remaining 3 milked 3-in-2 for either the whole lactation, after December 1 when cows were an average of 101 d in milk, or after March 1 when days in milk averaged 189 d. Milking intervals over 48 h were 10-14-10-14 h for TAD and 12-18-18 h for 3-in-2.					
35180910	0	0	theme	Immune	100:105	arg1	Response					107:114	Immune Response	100:114	Immune Response	100:114	Hyaluronic Acid-Melatonin Nanoparticles Improve the Dysregulated Intestinal Barrier, Microbiome and Immune Response in Mice with Dextran Sodium Sulfate-Induced Colitis.					
35180910	1	1	theme	mucosal	335:341	arg1	response					350:357	mucosal immune response	335:357	mucosal immune response	335:357	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	3	2	theme	inflamed	691:698	arg1	epithelium					706:715	the inflamed colon epithelium	687:715	the inflamed colon epithelium of colitis mice	687:731	Inducing colitis by dextran sodium sulfate (DSS), HA-MT accumulated in the inflamed colon epithelium of colitis mice, and markedly improved the colitis symptoms, repaired the damaged intestinal barrier and inhibited colon inflammation.					
35180910	1	3	theme	immune	343:348	arg1	response					350:357	mucosal immune response	335:357	mucosal immune response	335:357	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	4	4	with	mice	1080:1083	arg1	colitis					1090:1096	colitis	1090:1096	colitis	1090:1096	In addition, through bacterial 16S rDNA sequencing, it was found that HA-MT can restore the ratio of Firmicutes/Bacteroidetes by increasing the overall microbial richness and diversity, and alleviate the intestinal dysbiosis of mice with colitis.					
35180910	4	5	theme	intestinal	1056:1065	arg1	dysbiosis					1067:1075	the intestinal dysbiosis	1052:1075	the intestinal dysbiosis of mice with colitis	1052:1096	In addition, through bacterial 16S rDNA sequencing, it was found that HA-MT can restore the ratio of Firmicutes/Bacteroidetes by increasing the overall microbial richness and diversity, and alleviate the intestinal dysbiosis of mice with colitis.					
35180910	5	6	theme	Lactobacillus	1178:1190	arg1	abundance					1165:1173	the abundance	1161:1173	the abundance of Lactobacillus	1161:1190	In the analysis of the intestinal flora at the species level, the abundance of Lactobacillus increased in colitis mice treated with HA-MT while that of Bacteroides, Blautia and Streptococcus decreased in the colitis mice treated with HA-MT.					
35180910	1	7	theme	response	350:357	arg1	imbalance					322:330	imbalance	322:330	imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier	322:422	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	4	8	theme	bacterial	873:881	arg1	sequencing					892:901	bacterial 16S rDNA sequencing	873:901	bacterial 16S rDNA sequencing	873:901	In addition, through bacterial 16S rDNA sequencing, it was found that HA-MT can restore the ratio of Firmicutes/Bacteroidetes by increasing the overall microbial richness and diversity, and alleviate the intestinal dysbiosis of mice with colitis.					
35180910	3	9	theme	intestinal	799:808	arg1	barrier					810:816	the damaged intestinal barrier	787:816	the damaged intestinal barrier	787:816	Inducing colitis by dextran sodium sulfate (DSS), HA-MT accumulated in the inflamed colon epithelium of colitis mice, and markedly improved the colitis symptoms, repaired the damaged intestinal barrier and inhibited colon inflammation.					
35180910	5	10	theme	colitis	1307:1313	arg1	mice					1315:1318	the colitis mice	1303:1318	the colitis mice treated with HA-MT	1303:1337	In the analysis of the intestinal flora at the species level, the abundance of Lactobacillus increased in colitis mice treated with HA-MT while that of Bacteroides, Blautia and Streptococcus decreased in the colitis mice treated with HA-MT.					
35180910	6	11	theme	intestinal	1501:1510	arg1	homeostasis					1512:1522	intestinal homeostasis	1501:1522	intestinal homeostasis	1501:1522	Our findings suggest that the HA-MT system is a promising prebiotic, which can relieve the symptoms of IBD by regulating the intestinal microflora and restoring intestinal homeostasis, inhibiting inflammation.					
35180910	1	12	theme	intestinal	360:369	arg1	dysbiosis					371:379	intestinal dysbiosis	360:379	intestinal dysbiosis	360:379	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	4	13	theme	overall	996:1002	arg1	richness					1014:1021	the overall microbial richness	992:1021	the overall microbial richness	992:1021	In addition, through bacterial 16S rDNA sequencing, it was found that HA-MT can restore the ratio of Firmicutes/Bacteroidetes by increasing the overall microbial richness and diversity, and alleviate the intestinal dysbiosis of mice with colitis.					
35180910	1	14	theme	dysbiosis	371:379	arg1	imbalance					322:330	imbalance	322:330	imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier	322:422	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	6	15	theme	promising	1388:1396	arg1	system					1376:1381	the HA-MT system	1366:1381	the HA-MT system	1366:1381	Our findings suggest that the HA-MT system is a promising prebiotic, which can relieve the symptoms of IBD by regulating the intestinal microflora and restoring intestinal homeostasis, inhibiting inflammation.					
35180910	6	15	theme	promising	1388:1396	arg1	prebiotic					1398:1406	a promising prebiotic	1386:1406	a promising prebiotic	1386:1406	Our findings suggest that the HA-MT system is a promising prebiotic, which can relieve the symptoms of IBD by regulating the intestinal microflora and restoring intestinal homeostasis, inhibiting inflammation.					
35180910	2	16	theme	amphiphilic	443:453	arg1	conjugate					455:463	an amphiphilic conjugate	440:463	an amphiphilic conjugate	440:463	We synthesized an amphiphilic conjugate of hyaluronic acid (HA) and melatonin (MT), which have established immunomodulatory and antioxidant properties, by stimulating their nano-aggregation.					
35180910	4	17	located	found	911:915	arg2	it					904:905	it	904:905	it	904:905	In addition, through bacterial 16S rDNA sequencing, it was found that HA-MT can restore the ratio of Firmicutes/Bacteroidetes by increasing the overall microbial richness and diversity, and alleviate the intestinal dysbiosis of mice with colitis.					
35180910	4	17	located	found	911:915	arg1	addition					855:862	addition	855:862	addition	855:862	In addition, through bacterial 16S rDNA sequencing, it was found that HA-MT can restore the ratio of Firmicutes/Bacteroidetes by increasing the overall microbial richness and diversity, and alleviate the intestinal dysbiosis of mice with colitis.					
35180910	1	18	theme	inflammatory	191:202	arg1	IBD					219:221	IBD	219:221	IBD	219:221	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	1	18	theme	inflammatory	191:202	arg1	disease					210:216	inflammatory bowel disease	191:216	inflammatory bowel disease (IBD)	191:222	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	0	19	theme	Acid-Melatonin	11:24	arg1	Nanoparticles					26:38	Hyaluronic Acid-Melatonin Nanoparticles	0:38	Hyaluronic Acid-Melatonin Nanoparticles	0:38	Hyaluronic Acid-Melatonin Nanoparticles Improve the Dysregulated Intestinal Barrier, Microbiome and Immune Response in Mice with Dextran Sodium Sulfate-Induced Colitis.					
35180910	4	20	theme	Firmicutes/Bacteroidetes	953:976	arg1	ratio					944:948	the ratio	940:948	the ratio of Firmicutes/Bacteroidetes	940:976	In addition, through bacterial 16S rDNA sequencing, it was found that HA-MT can restore the ratio of Firmicutes/Bacteroidetes by increasing the overall microbial richness and diversity, and alleviate the intestinal dysbiosis of mice with colitis.					
35180910	5	21	from	level	1154:1158	arg1	analysis					1106:1113	the analysis	1102:1113	the analysis of the intestinal flora at the species level	1102:1158	In the analysis of the intestinal flora at the species level, the abundance of Lactobacillus increased in colitis mice treated with HA-MT while that of Bacteroides, Blautia and Streptococcus decreased in the colitis mice treated with HA-MT.					
35180910	0	22	with	Mice	119:122	arg1	Colitis					160:166	Dextran Sodium Sulfate-Induced Colitis	129:166	Dextran Sodium Sulfate-Induced Colitis	129:166	Hyaluronic Acid-Melatonin Nanoparticles Improve the Dysregulated Intestinal Barrier, Microbiome and Immune Response in Mice with Dextran Sodium Sulfate-Induced Colitis.					
35180910	3	23	theme	colitis	720:726	arg1	mice					728:731	colitis mice	720:731	colitis mice	720:731	Inducing colitis by dextran sodium sulfate (DSS), HA-MT accumulated in the inflamed colon epithelium of colitis mice, and markedly improved the colitis symptoms, repaired the damaged intestinal barrier and inhibited colon inflammation.					
35180910	1	24	theme	bowel	204:208	arg1	IBD					219:221	IBD	219:221	IBD	219:221	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	1	24	theme	bowel	204:208	arg1	disease					210:216	inflammatory bowel disease	191:216	inflammatory bowel disease (IBD)	191:222	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	0	25	theme	Hyaluronic	0:9	arg1	Nanoparticles					26:38	Hyaluronic Acid-Melatonin Nanoparticles	0:38	Hyaluronic Acid-Melatonin Nanoparticles	0:38	Hyaluronic Acid-Melatonin Nanoparticles Improve the Dysregulated Intestinal Barrier, Microbiome and Immune Response in Mice with Dextran Sodium Sulfate-Induced Colitis.					
35180910	1	26	theme	main	272:275	arg1	factors					277:283	the main factors	268:283	the main factors involved in its pathogenesis	268:312	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	1	27	theme	disease	210:216	arg1	unclear					227:233	unclear	227:233	unclear	227:233	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	1	27	theme	disease	210:216	arg1	cause					182:186	the cause	178:186	the cause of inflammatory bowel disease (IBD)	178:222	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	2	28	theme	immunomodulatory	532:547	arg1	properties					565:574	immunomodulatory and antioxidant properties	532:574	immunomodulatory and antioxidant properties	532:574	We synthesized an amphiphilic conjugate of hyaluronic acid (HA) and melatonin (MT), which have established immunomodulatory and antioxidant properties, by stimulating their nano-aggregation.					
35180910	3	29	theme	mice	728:731	arg1	epithelium					706:715	the inflamed colon epithelium	687:715	the inflamed colon epithelium of colitis mice	687:731	Inducing colitis by dextran sodium sulfate (DSS), HA-MT accumulated in the inflamed colon epithelium of colitis mice, and markedly improved the colitis symptoms, repaired the damaged intestinal barrier and inhibited colon inflammation.					
35180910	6	30	theme	intestinal	1465:1474	arg1	microflora					1476:1485	the intestinal microflora	1461:1485	the intestinal microflora	1461:1485	Our findings suggest that the HA-MT system is a promising prebiotic, which can relieve the symptoms of IBD by regulating the intestinal microflora and restoring intestinal homeostasis, inhibiting inflammation.					
35180910	0	31	theme	Dysregulated	52:63	arg1	Barrier					76:82	the Dysregulated Intestinal Barrier	48:82	the Dysregulated Intestinal Barrier	48:82	Hyaluronic Acid-Melatonin Nanoparticles Improve the Dysregulated Intestinal Barrier, Microbiome and Immune Response in Mice with Dextran Sodium Sulfate-Induced Colitis.					
35180910	1	32	theme	destruction	386:396	arg1	imbalance					322:330	imbalance	322:330	imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier	322:422	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	6	33	theme	IBD	1443:1445	arg1	symptoms					1431:1438	the symptoms	1427:1438	the symptoms of IBD	1427:1445	Our findings suggest that the HA-MT system is a promising prebiotic, which can relieve the symptoms of IBD by regulating the intestinal microflora and restoring intestinal homeostasis, inhibiting inflammation.					
35180910	2	34	theme	acid	479:482	arg1	conjugate					455:463	an amphiphilic conjugate	440:463	an amphiphilic conjugate	440:463	We synthesized an amphiphilic conjugate of hyaluronic acid (HA) and melatonin (MT), which have established immunomodulatory and antioxidant properties, by stimulating their nano-aggregation.					
35180910	3	35	theme	colitis	760:766	arg1	symptoms					768:775	the colitis symptoms	756:775	the colitis symptoms	756:775	Inducing colitis by dextran sodium sulfate (DSS), HA-MT accumulated in the inflamed colon epithelium of colitis mice, and markedly improved the colitis symptoms, repaired the damaged intestinal barrier and inhibited colon inflammation.					
35180910	0	36	theme	Sulfate-Induced	144:158	arg1	Colitis					160:166	Dextran Sodium Sulfate-Induced Colitis	129:166	Dextran Sodium Sulfate-Induced Colitis	129:166	Hyaluronic Acid-Melatonin Nanoparticles Improve the Dysregulated Intestinal Barrier, Microbiome and Immune Response in Mice with Dextran Sodium Sulfate-Induced Colitis.					
35180910	3	37	theme	dextran	636:642	arg1	sulfate					651:657	dextran sodium sulfate	636:657	dextran sodium sulfate (DSS)	636:663	Inducing colitis by dextran sodium sulfate (DSS), HA-MT accumulated in the inflamed colon epithelium of colitis mice, and markedly improved the colitis symptoms, repaired the damaged intestinal barrier and inhibited colon inflammation.					
35180910	3	37	theme	dextran	636:642	arg1	DSS					660:662	DSS	660:662	DSS	660:662	Inducing colitis by dextran sodium sulfate (DSS), HA-MT accumulated in the inflamed colon epithelium of colitis mice, and markedly improved the colitis symptoms, repaired the damaged intestinal barrier and inhibited colon inflammation.					
35180910	2	38	theme	hyaluronic	468:477	arg1	HA					485:486	HA	485:486	HA	485:486	We synthesized an amphiphilic conjugate of hyaluronic acid (HA) and melatonin (MT), which have established immunomodulatory and antioxidant properties, by stimulating their nano-aggregation.					
35180910	2	38	theme	hyaluronic	468:477	arg1	acid					479:482	hyaluronic acid	468:482	hyaluronic acid (HA)	468:487	We synthesized an amphiphilic conjugate of hyaluronic acid (HA) and melatonin (MT), which have established immunomodulatory and antioxidant properties, by stimulating their nano-aggregation.					
35180910	5	39	theme	flora	1133:1137	arg1	analysis					1106:1113	the analysis	1102:1113	the analysis of the intestinal flora at the species level	1102:1158	In the analysis of the intestinal flora at the species level, the abundance of Lactobacillus increased in colitis mice treated with HA-MT while that of Bacteroides, Blautia and Streptococcus decreased in the colitis mice treated with HA-MT.					
35180910	4	40	theme	rDNA	887:890	arg1	sequencing					892:901	bacterial 16S rDNA sequencing	873:901	bacterial 16S rDNA sequencing	873:901	In addition, through bacterial 16S rDNA sequencing, it was found that HA-MT can restore the ratio of Firmicutes/Bacteroidetes by increasing the overall microbial richness and diversity, and alleviate the intestinal dysbiosis of mice with colitis.					
35180910	3	41	theme	sodium	644:649	arg1	sulfate					651:657	dextran sodium sulfate	636:657	dextran sodium sulfate (DSS)	636:663	Inducing colitis by dextran sodium sulfate (DSS), HA-MT accumulated in the inflamed colon epithelium of colitis mice, and markedly improved the colitis symptoms, repaired the damaged intestinal barrier and inhibited colon inflammation.					
35180910	3	41	theme	sodium	644:649	arg1	DSS					660:662	DSS	660:662	DSS	660:662	Inducing colitis by dextran sodium sulfate (DSS), HA-MT accumulated in the inflamed colon epithelium of colitis mice, and markedly improved the colitis symptoms, repaired the damaged intestinal barrier and inhibited colon inflammation.					
35180910	3	42	theme	colon	700:704	arg1	epithelium					706:715	the inflamed colon epithelium	687:715	the inflamed colon epithelium of colitis mice	687:731	Inducing colitis by dextran sodium sulfate (DSS), HA-MT accumulated in the inflamed colon epithelium of colitis mice, and markedly improved the colitis symptoms, repaired the damaged intestinal barrier and inhibited colon inflammation.					
35180910	0	43	theme	Intestinal	65:74	arg1	Barrier					76:82	the Dysregulated Intestinal Barrier	48:82	the Dysregulated Intestinal Barrier	48:82	Hyaluronic Acid-Melatonin Nanoparticles Improve the Dysregulated Intestinal Barrier, Microbiome and Immune Response in Mice with Dextran Sodium Sulfate-Induced Colitis.					
35180910	4	44	theme	16S	883:885	arg1	sequencing					892:901	bacterial 16S rDNA sequencing	873:901	bacterial 16S rDNA sequencing	873:901	In addition, through bacterial 16S rDNA sequencing, it was found that HA-MT can restore the ratio of Firmicutes/Bacteroidetes by increasing the overall microbial richness and diversity, and alleviate the intestinal dysbiosis of mice with colitis.					
35180910	1	45	theme	intestinal	405:414	arg1	barrier					416:422	the intestinal barrier	401:422	the intestinal barrier	401:422	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	5	46	theme	species	1146:1152	arg1	level					1154:1158	the species level	1142:1158	the species level	1142:1158	In the analysis of the intestinal flora at the species level, the abundance of Lactobacillus increased in colitis mice treated with HA-MT while that of Bacteroides, Blautia and Streptococcus decreased in the colitis mice treated with HA-MT.					
35180910	2	47	theme	antioxidant	553:563	arg1	properties					565:574	immunomodulatory and antioxidant properties	532:574	immunomodulatory and antioxidant properties	532:574	We synthesized an amphiphilic conjugate of hyaluronic acid (HA) and melatonin (MT), which have established immunomodulatory and antioxidant properties, by stimulating their nano-aggregation.					
35180910	1	48	theme	barrier	416:422	arg1	response					350:357	mucosal immune response	335:357	mucosal immune response	335:357	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	1	48	theme	barrier	416:422	arg1	dysbiosis					371:379	intestinal dysbiosis	360:379	intestinal dysbiosis	360:379	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	1	48	theme	barrier	416:422	arg1	destruction					386:396	destruction	386:396	destruction of the intestinal barrier	386:422	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	5	49	theme	colitis	1205:1211	arg1	mice					1213:1216	colitis mice	1205:1216	colitis mice treated with HA-MT	1205:1235	In the analysis of the intestinal flora at the species level, the abundance of Lactobacillus increased in colitis mice treated with HA-MT while that of Bacteroides, Blautia and Streptococcus decreased in the colitis mice treated with HA-MT.					
35180910	6	50	theme	HA-MT	1370:1374	arg1	system					1376:1381	the HA-MT system	1366:1381	the HA-MT system	1366:1381	Our findings suggest that the HA-MT system is a promising prebiotic, which can relieve the symptoms of IBD by regulating the intestinal microflora and restoring intestinal homeostasis, inhibiting inflammation.					
35180910	6	50	theme	HA-MT	1370:1374	arg1	prebiotic					1398:1406	a promising prebiotic	1386:1406	a promising prebiotic	1386:1406	Our findings suggest that the HA-MT system is a promising prebiotic, which can relieve the symptoms of IBD by regulating the intestinal microflora and restoring intestinal homeostasis, inhibiting inflammation.					
35180910	3	51	theme	colon	832:836	arg1	inflammation					838:849	colon inflammation	832:849	colon inflammation	832:849	Inducing colitis by dextran sodium sulfate (DSS), HA-MT accumulated in the inflamed colon epithelium of colitis mice, and markedly improved the colitis symptoms, repaired the damaged intestinal barrier and inhibited colon inflammation.					
35180910	3	52	theme	damaged	791:797	arg1	barrier					810:816	the damaged intestinal barrier	787:816	the damaged intestinal barrier	787:816	Inducing colitis by dextran sodium sulfate (DSS), HA-MT accumulated in the inflamed colon epithelium of colitis mice, and markedly improved the colitis symptoms, repaired the damaged intestinal barrier and inhibited colon inflammation.					
35180910	2	53	theme	melatonin	493:501	arg1	conjugate					455:463	an amphiphilic conjugate	440:463	an amphiphilic conjugate	440:463	We synthesized an amphiphilic conjugate of hyaluronic acid (HA) and melatonin (MT), which have established immunomodulatory and antioxidant properties, by stimulating their nano-aggregation.					
35180910	5	54	theme	intestinal	1122:1131	arg1	flora					1133:1137	the intestinal flora	1118:1137	the intestinal flora	1118:1137	In the analysis of the intestinal flora at the species level, the abundance of Lactobacillus increased in colitis mice treated with HA-MT while that of Bacteroides, Blautia and Streptococcus decreased in the colitis mice treated with HA-MT.					
35180910	4	55	theme	mice	1080:1083	arg1	dysbiosis					1067:1075	the intestinal dysbiosis	1052:1075	the intestinal dysbiosis of mice with colitis	1052:1096	In addition, through bacterial 16S rDNA sequencing, it was found that HA-MT can restore the ratio of Firmicutes/Bacteroidetes by increasing the overall microbial richness and diversity, and alleviate the intestinal dysbiosis of mice with colitis.					
35180910	1	56	theme	current	236:242	arg1	studies					244:250	current studies	236:250	current studies	236:250	Although the cause of inflammatory bowel disease (IBD) is unclear, current studies have found that the main factors involved in its pathogenesis include imbalance of mucosal immune response, intestinal dysbiosis, and destruction of the intestinal barrier.					
35180910	4	57	theme	microbial	1004:1012	arg1	richness					1014:1021	the overall microbial richness	992:1021	the overall microbial richness	992:1021	In addition, through bacterial 16S rDNA sequencing, it was found that HA-MT can restore the ratio of Firmicutes/Bacteroidetes by increasing the overall microbial richness and diversity, and alleviate the intestinal dysbiosis of mice with colitis.					
36264059	4	0	theme	LPS	969:971	arg1	challenge					973:981	secondary LPS challenge	959:981	secondary LPS challenge	959:981	We find KD alters the composition of the hematopoietic stem cell compartment and enhances the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge.					
36264059	10	1	theme	reprogramming	1902:1914	arg1	plasticity					1869:1878	the plasticity	1865:1878	the plasticity of this innate immune reprogramming	1865:1914	</i> These implicate enriched dietary SFAs, and specifically PA, in the induction of long-lived innate immune memory and highlight the plasticity of this innate immune reprogramming by dietary constituents.					
36264059	4	2	theme	stem	853:856	arg1	compartment					863:873	the hematopoietic stem cell compartment	835:873	the hematopoietic stem cell compartment	835:873	We find KD alters the composition of the hematopoietic stem cell compartment and enhances the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge.					
36264059	10	3	theme	dietary	1919:1925	arg1	constituents					1927:1938	dietary constituents	1919:1938	dietary constituents	1919:1938	</i> These implicate enriched dietary SFAs, and specifically PA, in the induction of long-lived innate immune memory and highlight the plasticity of this innate immune reprogramming by dietary constituents.					
36264059	3	4	dep	find	553:556	arg1	confer					641:646	confer	641:646	find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia	553:795	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	4	5	theme	secondary	959:967	arg1	challenge					973:981	secondary LPS challenge	959:981	secondary LPS challenge	959:981	We find KD alters the composition of the hematopoietic stem cell compartment and enhances the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge.					
36264059	1	6	theme	host	354:357	arg1	response					359:366	the host response	350:366	the host response to infection and inflammatory disease	350:404	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	4	7	theme	hematopoietic	839:851	arg1	compartment					863:873	the hematopoietic stem cell compartment	835:873	the hematopoietic stem cell compartment	835:873	We find KD alters the composition of the hematopoietic stem cell compartment and enhances the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge.					
36264059	9	8	theme	PA-induced	1644:1653	arg1	hyper-inflammation					1655:1672	PA-induced hyper-inflammation	1644:1672	PA-induced hyper-inflammation in macrophages	1644:1687	Lastly, we show that oleic acid, which depletes intracellular ceramide, reverses PA-induced hyper-inflammation in macrophages and enhanced mortality in response to LPS<i>.					
36264059	8	9	theme	PA	1470:1471	arg1	exposure					1473:1480	PA exposure	1470:1480	PA exposure	1470:1480	Conversely, we find PA exposure enhanced clearance of <i>Candida albicans</i> in <i>Rag1</i><sup>-/-</sup> mice.					
36264059	5	10	theme	PA-associated	1043:1055	arg1	lipids					1057:1062	PA-associated lipids	1043:1062	PA-associated lipids	1043:1062	Lipidomics identified enhanced free palmitic acid (PA) and PA-associated lipids in KD-fed mice serum.					
36264059	4	11	theme	monocytes	929:937	arg1	response					892:899	the response	888:899	the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge	888:981	We find KD alters the composition of the hematopoietic stem cell compartment and enhances the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge.					
36264059	3	12	theme	hyper-inflammatory	650:667	arg1	response					669:676	a hyper-inflammatory response	648:676	a hyper-inflammatory response	648:676	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	4	13	theme	compartment	863:873	arg1	composition					820:830	the composition	816:830	the composition of the hematopoietic stem cell compartment	816:873	We find KD alters the composition of the hematopoietic stem cell compartment and enhances the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge.					
36264059	9	14	theme	oleic	1584:1588	arg1	acid					1590:1593	oleic acid	1584:1593	oleic acid	1584:1593	Lastly, we show that oleic acid, which depletes intracellular ceramide, reverses PA-induced hyper-inflammation in macrophages and enhanced mortality in response to LPS<i>.					
36264059	10	15	theme	dietary	1764:1770	arg1	SFAs					1772:1775	enriched dietary SFAs	1755:1775	enriched dietary SFAs	1755:1775	</i> These implicate enriched dietary SFAs, and specifically PA, in the induction of long-lived innate immune memory and highlight the plasticity of this innate immune reprogramming by dietary constituents.					
36264059	5	16	from	serum	1079:1083	arg1	mice					1074:1077	KD-fed mice	1067:1077	KD-fed mice	1067:1077	Lipidomics identified enhanced free palmitic acid (PA) and PA-associated lipids in KD-fed mice serum.					
36264059	4	17	theme	cell	858:861	arg1	compartment					863:873	the hematopoietic stem cell compartment	835:873	the hematopoietic stem cell compartment	835:873	We find KD alters the composition of the hematopoietic stem cell compartment and enhances the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge.					
36264059	9	18	theme	intracellular	1611:1623	arg1	ceramide					1625:1632	intracellular ceramide	1611:1632	intracellular ceramide	1611:1632	Lastly, we show that oleic acid, which depletes intracellular ceramide, reverses PA-induced hyper-inflammation in macrophages and enhanced mortality in response to LPS<i>.					
36264059	6	19	theme	hyper-inflammatory	1170:1187	arg1	response					1189:1196	a hyper-inflammatory response	1168:1196	a hyper-inflammatory response to LPS in macrophages	1168:1218	We found pre-treatment with physiologically relevant concentrations of PA induces a hyper-inflammatory response to LPS in macrophages, and this was dependent on the synthesis of ceramide.					
36264059	3	20	dep	diet	631:634	arg1	KD					637:638	KD	637:638	ketogenic diet; KD	621:638	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	2	21	theme	primary	501:507	arg1	they					481:484	they	481:484	they	481:484	Dietary fatty acids can act as inflammatory stimuli, but it is unknown if they can act as the primary stimuli to induce trained immunity.					
36264059	2	21	theme	primary	501:507	arg1	stimuli					509:515	the primary stimuli	497:515	the primary stimuli	497:515	Dietary fatty acids can act as inflammatory stimuli, but it is unknown if they can act as the primary stimuli to induce trained immunity.					
36264059	7	22	from	inflammation	1321:1332	arg1	response					1351:1358	response	1351:1358	response to systemic LPS	1351:1374	In vivo, we found systemic PA confers enhanced inflammation and mortality in response to systemic LPS, and this phenotype was not reversible for up to 7 days post-PA-exposure.					
36264059	7	23	theme	systemic	1292:1299	arg1	PA					1301:1302	systemic PA	1292:1302	systemic PA	1292:1302	In vivo, we found systemic PA confers enhanced inflammation and mortality in response to systemic LPS, and this phenotype was not reversible for up to 7 days post-PA-exposure.					
36264059	2	24	theme	fatty	415:419	arg1	stimuli					451:457	inflammatory stimuli	438:457	inflammatory stimuli	438:457	Dietary fatty acids can act as inflammatory stimuli, but it is unknown if they can act as the primary stimuli to induce trained immunity.					
36264059	2	24	theme	fatty	415:419	arg1	acids					421:425	Dietary fatty acids	407:425	Dietary fatty acids	407:425	Dietary fatty acids can act as inflammatory stimuli, but it is unknown if they can act as the primary stimuli to induce trained immunity.					
36264059	9	25	dep	LPS<i>	1727:1732	arg1	response					1715:1722	response	1715:1722	response	1715:1722	Lastly, we show that oleic acid, which depletes intracellular ceramide, reverses PA-induced hyper-inflammation in macrophages and enhanced mortality in response to LPS<i>.					
36264059	3	26	theme	systemic	681:688	arg1	lipopolysaccharide					690:707	systemic lipopolysaccharide	681:707	systemic lipopolysaccharide (LPS)	681:713	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	3	26	theme	systemic	681:688	arg1	LPS					710:712	LPS	710:712	LPS	710:712	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	0	27	theme	Enriched	0:7	arg1	acids					33:37	Enriched dietary saturated fatty acids	0:37	Enriched dietary saturated fatty acids	0:37	Enriched dietary saturated fatty acids induce trained immunity via ceramide production that enhances severity of endotoxemia and clearance of infection.					
36264059	4	28	theme	macrophages	916:926	arg1	response					892:899	the response	888:899	the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge	888:981	We find KD alters the composition of the hematopoietic stem cell compartment and enhances the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge.					
36264059	2	29	theme	Dietary	407:413	arg1	stimuli					451:457	inflammatory stimuli	438:457	inflammatory stimuli	438:457	Dietary fatty acids can act as inflammatory stimuli, but it is unknown if they can act as the primary stimuli to induce trained immunity.					
36264059	2	29	theme	Dietary	407:413	arg1	acids					421:425	Dietary fatty acids	407:425	Dietary fatty acids	407:425	Dietary fatty acids can act as inflammatory stimuli, but it is unknown if they can act as the primary stimuli to induce trained immunity.					
36264059	0	30	theme	dietary	9:15	arg1	acids					33:37	Enriched dietary saturated fatty acids	0:37	Enriched dietary saturated fatty acids	0:37	Enriched dietary saturated fatty acids induce trained immunity via ceramide production that enhances severity of endotoxemia and clearance of infection.					
36264059	1	31	theme	inflammatory	385:396	arg1	disease					398:404	inflammatory disease	385:404	inflammatory disease	385:404	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	6	32	theme	PA	1157:1158	arg1	concentrations					1139:1152	physiologically relevant concentrations	1114:1152	physiologically relevant concentrations of PA	1114:1158	We found pre-treatment with physiologically relevant concentrations of PA induces a hyper-inflammatory response to LPS in macrophages, and this was dependent on the synthesis of ceramide.					
36264059	1	33	theme	immune	183:188	arg1	immunity					161:168	Trained immunity	153:168	Trained immunity	153:168	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	1	33	theme	immune	183:188	arg1	response					197:204	an innate immune memory response	173:204	an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease	173:404	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	0	34	theme	infection	142:150	arg1	severity					101:108	severity	101:108	severity of endotoxemia	101:123	Enriched dietary saturated fatty acids induce trained immunity via ceramide production that enhances severity of endotoxemia and clearance of infection.					
36264059	0	34	theme	infection	142:150	arg1	clearance					129:137	clearance	129:137	clearance of infection	129:150	Enriched dietary saturated fatty acids induce trained immunity via ceramide production that enhances severity of endotoxemia and clearance of infection.					
36264059	3	35	theme	increased	719:727	arg1	mortality					729:737	increased mortality	719:737	increased mortality	719:737	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	10	36	theme	immune	1837:1842	arg1	memory					1844:1849	long-lived innate immune memory	1819:1849	long-lived innate immune memory	1819:1849	</i> These implicate enriched dietary SFAs, and specifically PA, in the induction of long-lived innate immune memory and highlight the plasticity of this innate immune reprogramming by dietary constituents.					
36264059	3	37	theme	diet-induced	755:766	arg1	microbiome					768:777	diet-induced microbiome	755:777	diet-induced microbiome	755:777	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	1	38	theme	memory	190:195	arg1	immunity					161:168	Trained immunity	153:168	Trained immunity	153:168	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	1	38	theme	memory	190:195	arg1	response					197:204	an innate immune memory response	173:204	an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease	173:404	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	0	39	theme	fatty	27:31	arg1	acids					33:37	Enriched dietary saturated fatty acids	0:37	Enriched dietary saturated fatty acids	0:37	Enriched dietary saturated fatty acids induce trained immunity via ceramide production that enhances severity of endotoxemia and clearance of infection.					
36264059	8	40	theme	albicans</i>	1515:1526	arg1	clearance					1491:1499	clearance	1491:1499	clearance of <i>Candida albicans</i> in <i>Rag1</i><sup>-/-</sup> mice	1491:1560	Conversely, we find PA exposure enhanced clearance of <i>Candida albicans</i> in <i>Rag1</i><sup>-/-</sup> mice.					
36264059	4	41	theme	bone	904:907	arg1	macrophages					916:926	bone marrow macrophages	904:926	bone marrow macrophages	904:926	We find KD alters the composition of the hematopoietic stem cell compartment and enhances the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge.					
36264059	7	42	from	mortality	1338:1346	arg1	response					1351:1358	response	1351:1358	response to systemic LPS	1351:1374	In vivo, we found systemic PA confers enhanced inflammation and mortality in response to systemic LPS, and this phenotype was not reversible for up to 7 days post-PA-exposure.					
36264059	5	43	theme	enhanced	1006:1013	arg1	PA					1035:1036	PA	1035:1036	PA	1035:1036	Lipidomics identified enhanced free palmitic acid (PA) and PA-associated lipids in KD-fed mice serum.					
36264059	5	43	theme	enhanced	1006:1013	arg1	acid					1029:1032	free palmitic acid	1015:1032	free palmitic acid (PA)	1015:1037	Lipidomics identified enhanced free palmitic acid (PA) and PA-associated lipids in KD-fed mice serum.					
36264059	2	44	theme	inflammatory	438:449	arg1	stimuli					451:457	inflammatory stimuli	438:457	inflammatory stimuli	438:457	Dietary fatty acids can act as inflammatory stimuli, but it is unknown if they can act as the primary stimuli to induce trained immunity.					
36264059	2	44	theme	inflammatory	438:449	arg1	acids					421:425	Dietary fatty acids	407:425	Dietary fatty acids	407:425	Dietary fatty acids can act as inflammatory stimuli, but it is unknown if they can act as the primary stimuli to induce trained immunity.					
36264059	9	45	from	hyper-inflammation	1655:1672	arg1	macrophages					1677:1687	macrophages	1677:1687	macrophages	1677:1687	Lastly, we show that oleic acid, which depletes intracellular ceramide, reverses PA-induced hyper-inflammation in macrophages and enhanced mortality in response to LPS<i>.					
36264059	3	46	theme	ketogenic	621:629	arg1	diet					569:572	a diet	567:572	a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD)	567:639	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	3	46	theme	ketogenic	621:629	arg1	diet					631:634	ketogenic diet	621:634	ketogenic diet; KD	621:638	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	6	47	theme	relevant	1130:1137	arg1	concentrations					1139:1152	physiologically relevant concentrations	1114:1152	physiologically relevant concentrations of PA	1114:1158	We found pre-treatment with physiologically relevant concentrations of PA induces a hyper-inflammatory response to LPS in macrophages, and this was dependent on the synthesis of ceramide.					
36264059	10	48	theme	enriched	1755:1762	arg1	SFAs					1772:1775	enriched dietary SFAs	1755:1775	enriched dietary SFAs	1755:1775	</i> These implicate enriched dietary SFAs, and specifically PA, in the induction of long-lived innate immune memory and highlight the plasticity of this innate immune reprogramming by dietary constituents.					
36264059	10	49	theme	immune	1895:1900	arg1	reprogramming					1902:1914	this innate immune reprogramming	1883:1914	this innate immune reprogramming	1883:1914	</i> These implicate enriched dietary SFAs, and specifically PA, in the induction of long-lived innate immune memory and highlight the plasticity of this innate immune reprogramming by dietary constituents.					
36264059	8	50	theme	<i>Rag1</i><sup>-/-</sup>	1531:1555	arg1	mice					1557:1560	<i>Rag1</i><sup>-/-</sup> mice	1531:1560	<i>Rag1</i><sup>-/-</sup> mice	1531:1560	Conversely, we find PA exposure enhanced clearance of <i>Candida albicans</i> in <i>Rag1</i><sup>-/-</sup> mice.					
36264059	2	51	theme	trained	527:533	arg1	immunity					535:542	trained immunity	527:542	trained immunity	527:542	Dietary fatty acids can act as inflammatory stimuli, but it is unknown if they can act as the primary stimuli to induce trained immunity.					
36264059	0	52	theme	endotoxemia	113:123	arg1	severity					101:108	severity	101:108	severity of endotoxemia	101:123	Enriched dietary saturated fatty acids induce trained immunity via ceramide production that enhances severity of endotoxemia and clearance of infection.					
36264059	0	52	theme	endotoxemia	113:123	arg1	clearance					129:137	clearance	129:137	clearance of infection	129:150	Enriched dietary saturated fatty acids induce trained immunity via ceramide production that enhances severity of endotoxemia and clearance of infection.					
36264059	1	53	theme	secondary	304:312	arg1	challenge					325:333	a secondary pathogenic challenge	302:333	a secondary pathogenic challenge	302:333	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	4	54	theme	marrow	909:914	arg1	macrophages					916:926	bone marrow macrophages	904:926	bone marrow macrophages	904:926	We find KD alters the composition of the hematopoietic stem cell compartment and enhances the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge.					
36264059	10	55	theme	memory	1844:1849	arg1	induction					1806:1814	the induction	1802:1814	the induction of long-lived innate immune memory	1802:1849	</i> These implicate enriched dietary SFAs, and specifically PA, in the induction of long-lived innate immune memory and highlight the plasticity of this innate immune reprogramming by dietary constituents.					
36264059	3	56	theme	microbiome	768:777	arg1	independent					740:750	independent	740:750	independent	740:750	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	7	57	theme	post-PA-exposure	1432:1447	arg1	days					1427:1430	up to 7 days post-PA-exposure	1419:1447	up to 7 days post-PA-exposure	1419:1447	In vivo, we found systemic PA confers enhanced inflammation and mortality in response to systemic LPS, and this phenotype was not reversible for up to 7 days post-PA-exposure.					
36264059	10	58	theme	innate	1888:1893	arg1	reprogramming					1902:1914	this innate immune reprogramming	1883:1914	this innate immune reprogramming	1883:1914	</i> These implicate enriched dietary SFAs, and specifically PA, in the induction of long-lived innate immune memory and highlight the plasticity of this innate immune reprogramming by dietary constituents.					
36264059	8	59	from	clearance	1491:1499	arg1	mice					1557:1560	<i>Rag1</i><sup>-/-</sup> mice	1531:1560	<i>Rag1</i><sup>-/-</sup> mice	1531:1560	Conversely, we find PA exposure enhanced clearance of <i>Candida albicans</i> in <i>Rag1</i><sup>-/-</sup> mice.					
36264059	1	60	theme	pathogenic	314:323	arg1	challenge					325:333	a secondary pathogenic challenge	302:333	a secondary pathogenic challenge	302:333	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	5	61	theme	KD-fed	1067:1072	arg1	mice					1074:1077	KD-fed mice	1067:1077	KD-fed mice	1067:1077	Lipidomics identified enhanced free palmitic acid (PA) and PA-associated lipids in KD-fed mice serum.					
36264059	5	62	theme	palmitic	1020:1027	arg1	PA					1035:1036	PA	1035:1036	PA	1035:1036	Lipidomics identified enhanced free palmitic acid (PA) and PA-associated lipids in KD-fed mice serum.					
36264059	5	62	theme	palmitic	1020:1027	arg1	acid					1029:1032	free palmitic acid	1015:1032	free palmitic acid (PA)	1015:1037	Lipidomics identified enhanced free palmitic acid (PA) and PA-associated lipids in KD-fed mice serum.					
36264059	8	63	theme	<i>Candida	1504:1513	arg1	albicans</i>					1515:1526	<i>Candida albicans</i>	1504:1526	<i>Candida albicans</i>	1504:1526	Conversely, we find PA exposure enhanced clearance of <i>Candida albicans</i> in <i>Rag1</i><sup>-/-</sup> mice.					
36264059	0	64	theme	trained	46:52	arg1	immunity					54:61	trained immunity	46:61	trained immunity	46:61	Enriched dietary saturated fatty acids induce trained immunity via ceramide production that enhances severity of endotoxemia and clearance of infection.					
36264059	7	65	dep	7	1425:1425	arg1	to					1422:1423	to	1422:1423	to	1422:1423	In vivo, we found systemic PA confers enhanced inflammation and mortality in response to systemic LPS, and this phenotype was not reversible for up to 7 days post-PA-exposure.					
36264059	7	66	theme	enhanced	1312:1319	arg1	inflammation					1321:1332	inflammation	1321:1332	inflammation	1321:1332	In vivo, we found systemic PA confers enhanced inflammation and mortality in response to systemic LPS, and this phenotype was not reversible for up to 7 days post-PA-exposure.					
36264059	6	67	theme	ceramide	1264:1271	arg1	synthesis					1251:1259	the synthesis	1247:1259	the synthesis of ceramide	1247:1271	We found pre-treatment with physiologically relevant concentrations of PA induces a hyper-inflammatory response to LPS in macrophages, and this was dependent on the synthesis of ceramide.					
36264059	3	68	theme	independent	740:750	arg1	response					669:676	a hyper-inflammatory response	648:676	a hyper-inflammatory response	648:676	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	10	69	theme	innate	1830:1835	arg1	memory					1844:1849	long-lived innate immune memory	1819:1849	long-lived innate immune memory	1819:1849	</i> These implicate enriched dietary SFAs, and specifically PA, in the induction of long-lived innate immune memory and highlight the plasticity of this innate immune reprogramming by dietary constituents.					
36264059	3	70	theme	saturated	598:606	arg1	acids					614:618	saturated fatty acids	598:618	saturated fatty acids	598:618	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	5	71	theme	free	1015:1018	arg1	PA					1035:1036	PA	1035:1036	PA	1035:1036	Lipidomics identified enhanced free palmitic acid (PA) and PA-associated lipids in KD-fed mice serum.					
36264059	5	71	theme	free	1015:1018	arg1	acid					1029:1032	free palmitic acid	1015:1032	free palmitic acid (PA)	1015:1037	Lipidomics identified enhanced free palmitic acid (PA) and PA-associated lipids in KD-fed mice serum.					
36264059	10	72	theme	long-lived	1819:1828	arg1	memory					1844:1849	long-lived innate immune memory	1819:1849	long-lived innate immune memory	1819:1849	</i> These implicate enriched dietary SFAs, and specifically PA, in the induction of long-lived innate immune memory and highlight the plasticity of this innate immune reprogramming by dietary constituents.					
36264059	3	73	theme	hyperglycemia	783:795	arg1	independent					740:750	independent	740:750	independent	740:750	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	3	74	theme	fatty	608:612	arg1	acids					614:618	saturated fatty acids	598:618	saturated fatty acids	598:618	Here we find mice fed a diet enriched exclusively in saturated fatty acids (ketogenic diet; KD) confer a hyper-inflammatory response to systemic lipopolysaccharide (LPS) and increased mortality, independent of diet-induced microbiome and hyperglycemia.					
36264059	7	75	theme	systemic	1363:1370	arg1	LPS					1372:1374	systemic LPS	1363:1374	systemic LPS	1363:1374	In vivo, we found systemic PA confers enhanced inflammation and mortality in response to systemic LPS, and this phenotype was not reversible for up to 7 days post-PA-exposure.					
36264059	6	76	from	response	1189:1196	arg1	macrophages					1208:1218	macrophages	1208:1218	macrophages	1208:1218	We found pre-treatment with physiologically relevant concentrations of PA induces a hyper-inflammatory response to LPS in macrophages, and this was dependent on the synthesis of ceramide.					
36264059	0	77	theme	ceramide	67:74	arg1	production					76:85	ceramide production	67:85	ceramide production that enhances severity of endotoxemia and clearance of infection	67:150	Enriched dietary saturated fatty acids induce trained immunity via ceramide production that enhances severity of endotoxemia and clearance of infection.					
36264059	1	78	theme	innate	176:181	arg1	immunity					161:168	Trained immunity	153:168	Trained immunity	153:168	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	1	78	theme	innate	176:181	arg1	response					197:204	an innate immune memory response	173:204	an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease	173:404	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	1	79	theme	primary	227:233	arg1	stimulus					248:255	a primary inflammatory stimulus	225:255	a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease	225:404	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	4	80	theme	splenocytes	944:954	arg1	response					892:899	the response	888:899	the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge	888:981	We find KD alters the composition of the hematopoietic stem cell compartment and enhances the response of bone marrow macrophages, monocytes, and splenocytes to secondary LPS challenge.					
36264059	6	81	with	pre-treatment	1095:1107	arg1	concentrations					1139:1152	physiologically relevant concentrations	1114:1152	physiologically relevant concentrations of PA	1114:1158	We found pre-treatment with physiologically relevant concentrations of PA induces a hyper-inflammatory response to LPS in macrophages, and this was dependent on the synthesis of ceramide.					
36264059	0	82	theme	saturated	17:25	arg1	acids					33:37	Enriched dietary saturated fatty acids	0:37	Enriched dietary saturated fatty acids	0:37	Enriched dietary saturated fatty acids induce trained immunity via ceramide production that enhances severity of endotoxemia and clearance of infection.					
36264059	1	83	theme	Trained	153:159	arg1	immunity					161:168	Trained immunity	153:168	Trained immunity	153:168	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	1	83	theme	Trained	153:159	arg1	response					197:204	an innate immune memory response	173:204	an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease	173:404	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
36264059	1	84	theme	inflammatory	235:246	arg1	stimulus					248:255	a primary inflammatory stimulus	225:255	a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease	225:404	Trained immunity is an innate immune memory response that is induced by a primary inflammatory stimulus that sensitizes monocytes and macrophages to a secondary pathogenic challenge, reprogramming the host response to infection and inflammatory disease.					
37002139	19	0	theme	calf	2650:2653	arg1	health					2655:2660	dairy calf health	2644:2660	dairy calf health	2644:2660	Understanding how MR composition affects dairy calf health may improve nutritional strategies on farm.					
37002139	16	1	theme	n-3	2229:2231	arg1	calves					2233:2238	n-3 calves	2229:2238	n-3 calves	2229:2238	Plasma concentrations of interleukin-4 were decreased in response to the ex vivo LPS challenge in n-3 calves.					
37002139	18	2	dep	proteins	2452:2459	arg1	haptoglobin					2461:2471	haptoglobin	2461:2471	haptoglobin	2461:2471	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	18	2	dep	proteins	2452:2459	arg1	A					2491:2491	serum amyloid A	2477:2491	serum amyloid A	2477:2491	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	18	2	dep	proteins	2452:2459	arg1	proteins					2452:2459	the acute-phase proteins	2436:2459	the acute-phase proteins haptoglobin and serum amyloid A postprandially	2436:2506	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	1	3	theme	intestinal	342:351	arg1	epithelium					353:362	the intestinal epithelium	338:362	the intestinal epithelium	338:362	The ratio of n-6 to n-3 fatty acid (FA) is between 2 and 10 times higher in milk replacer (MR) than in whole milk, which may promote inflammation and compromise the integrity of the intestinal epithelium.					
37002139	7	4	from	cells	1164:1168	arg1	secretion					1143:1151	cytokine secretion	1134:1151	cytokine secretion from blood cells	1134:1168	Whole blood collected 4 h after the meal was subjected to an ex vivo lipopolysaccharide (LPS) challenge to evaluate cytokine secretion from blood cells.					
37002139	14	5	theme	jejunum	1972:1978	arg1	tissue					1980:1985	proximal jejunum tissue	1963:1985	proximal jejunum tissue	1963:1985	Expression of TJP1 tended to be higher in proximal jejunum tissue and lower in ileum tissue of n-3 calves.					
37002139	2	6	dep	6.5:1	665:669	arg1	=					702:702	=	702:702	=	702:702	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	5	7	theme	GIT	887:889	arg1	permeability					891:902	GIT permeability	887:902	GIT permeability	887:902	On d 22, calves were administered Cr-EDTA, lactulose, and d-mannitol to assess GIT permeability.					
37002139	9	8	theme	gas	1305:1307	arg1	chromatography					1309:1322	gas chromatography	1305:1322	gas chromatography	1305:1322	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	2	9	dep	%	635:635	arg1	fat					643:645	crude fat	637:645	29.3% crude fat of DM	631:651	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	12	10	theme	lower	1728:1732	arg1	ratios					1734:1739	2-fold lower ratios	1721:1739	2-fold lower ratios	1721:1739	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	12	11	dep	n-3	1751:1753	arg1	to					1748:1749	to	1748:1749	to	1748:1749	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	7	12	theme	lipopolysaccharide	1087:1104	arg1	challenge					1112:1120	an ex vivo lipopolysaccharide (LPS) challenge	1076:1120	an ex vivo lipopolysaccharide (LPS) challenge	1076:1120	Whole blood collected 4 h after the meal was subjected to an ex vivo lipopolysaccharide (LPS) challenge to evaluate cytokine secretion from blood cells.					
37002139	2	13	theme	inflammatory	467:478	arg1	status					480:485	inflammatory status	467:485	inflammatory status	467:485	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	2	14	dep	=	702:702	arg1	%					681:681	n-3; 29.1%	672:681	n-3; 29.1% crude fat of DM	672:697	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	6	15	theme	total	915:919	arg1	urine					921:925	total urine	915:925	total urine	915:925	Blood and total urine were sequentially collected for 6 and 24 h, respectively, and analyzed for marker content.					
37002139	14	16	theme	TJP1	1935:1938	arg1	Expression					1921:1930	Expression	1921:1930	Expression of TJP1	1921:1938	Expression of TJP1 tended to be higher in proximal jejunum tissue and lower in ileum tissue of n-3 calves.					
37002139	9	17	theme	FA	1287:1288	arg1	composition					1290:1300	FA composition	1287:1300	FA composition	1287:1300	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	16	18	theme	Plasma	2131:2136	arg1	concentrations					2138:2151	Plasma concentrations	2131:2151	Plasma concentrations of interleukin-4	2131:2168	Plasma concentrations of interleukin-4 were decreased in response to the ex vivo LPS challenge in n-3 calves.					
37002139	2	19	theme	FA	608:609	arg1	ratio					611:615	an n-6:n-3 FA ratio	597:615	an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15)	597:706	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	12	20	theme	FA	1705:1706	arg1	contents					1708:1715	2-fold higher n-3 FA contents	1687:1715	2-fold higher n-3 FA contents	1687:1715	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	0	21	theme	n-6	120:122	arg1	ratio					111:115	the ratio	107:115	the ratio of n-6 to n-3 fatty acid in milk replacer	107:157	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	12	22	dep	jejunum	1770:1776	arg1	tissues					1788:1794	tissues	1788:1794	tissues	1788:1794	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	0	23	theme	n-3	127:129	arg1	acid					137:140	n-3 fatty acid	127:140	n-3 fatty acid in milk replacer	127:157	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	18	24	theme	proteins	2452:2459	arg1	concentrations					2418:2431	greater concentrations	2410:2431	greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially	2410:2506	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	14	25	theme	proximal	1963:1970	arg1	tissue					1980:1985	proximal jejunum tissue	1963:1985	proximal jejunum tissue	1963:1985	Expression of TJP1 tended to be higher in proximal jejunum tissue and lower in ileum tissue of n-3 calves.					
37002139	1	26	theme	fatty	184:188	arg1	FA					196:197	FA	196:197	FA	196:197	The ratio of n-6 to n-3 fatty acid (FA) is between 2 and 10 times higher in milk replacer (MR) than in whole milk, which may promote inflammation and compromise the integrity of the intestinal epithelium.					
37002139	1	26	theme	fatty	184:188	arg1	acid					190:193	n-3 fatty acid	180:193	n-3 fatty acid (FA)	180:198	The ratio of n-6 to n-3 fatty acid (FA) is between 2 and 10 times higher in milk replacer (MR) than in whole milk, which may promote inflammation and compromise the integrity of the intestinal epithelium.					
37002139	10	27	from	GLIMMIX	1525:1531	arg1	SAS					1536:1538	SAS	1536:1538	SAS (version 9.4, SAS Institute Inc.)	1536:1572	Data were analyzed using PROC GLIMMIX in SAS (version 9.4, SAS Institute Inc.).					
37002139	11	28	theme	Growth	1575:1580	arg1	performance					1582:1592	Growth performance	1575:1592	Growth performance	1575:1592	Growth performance and fecal consistency were unaffected.					
37002139	12	29	theme	higher	1694:1699	arg1	contents					1708:1715	2-fold higher n-3 FA contents	1687:1715	2-fold higher n-3 FA contents	1687:1715	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	0	30	theme	milk	145:148	arg1	replacer					150:157	milk replacer	145:157	milk replacer	145:157	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	18	31	theme	amyloid	2483:2489	arg1	A					2491:2491	serum amyloid A	2477:2491	serum amyloid A	2477:2491	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	18	31	theme	amyloid	2483:2489	arg1	proteins					2452:2459	the acute-phase proteins	2436:2459	the acute-phase proteins haptoglobin and serum amyloid A postprandially	2436:2506	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	7	32	dep	ex	1079:1080	arg1	vivo					1082:1085	vivo	1082:1085	vivo	1082:1085	Whole blood collected 4 h after the meal was subjected to an ex vivo lipopolysaccharide (LPS) challenge to evaluate cytokine secretion from blood cells.					
37002139	9	33	theme	tight	1392:1396	arg1	proteins					1407:1414	tight junction proteins	1392:1414	tight junction proteins	1392:1414	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	18	34	theme	linseed	2346:2352	arg1	inclusion					2333:2341	the inclusion	2329:2341	the inclusion of linseed and algae oil	2329:2366	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	0	35	theme	calves	74:79	arg1	permeability					17:28	Gastrointestinal permeability	0:28	Gastrointestinal permeability	0:28	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	0	35	theme	calves	74:79	arg1	status					47:52	inflammatory status	34:52	inflammatory status	34:52	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	13	36	theme	relative	1828:1835	arg1	h					1826:1826	0-24 h	1821:1826	0-24 h relative to marker administration	1821:1860	Total urinary recovery (0-24 h relative to marker administration) and plasma concentrations of the markers were unaffected.					
37002139	9	37	theme	proteins	1407:1414	arg1	chromatography					1309:1322	gas chromatography	1305:1322	gas chromatography	1305:1322	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	9	37	theme	proteins	1407:1414	arg1	expression					1378:1387	gene expression	1373:1387	gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules	1373:1462	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	9	37	theme	proteins	1407:1414	arg1	histomorphology					1325:1339	histomorphology	1325:1339	histomorphology by bright-field microscopy	1325:1366	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	2	38	theme	DM	696:697	arg1	fat					689:691	crude fat	683:691	n-3; 29.1% crude fat of DM	672:697	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	13	39	theme	marker	1840:1845	arg1	administration					1847:1860	marker administration	1840:1860	marker administration	1840:1860	Total urinary recovery (0-24 h relative to marker administration) and plasma concentrations of the markers were unaffected.					
37002139	9	40	theme	lipid	1417:1421	arg1	enzymes					1434:1440	lipid metabolism enzymes	1417:1440	lipid metabolism enzymes	1417:1440	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	13	41	dep	recovery	1811:1818	arg1	h					1826:1826	0-24 h	1821:1826	0-24 h relative to marker administration	1821:1860	Total urinary recovery (0-24 h relative to marker administration) and plasma concentrations of the markers were unaffected.					
37002139	2	42	theme	age	521:523	arg1	d					516:516	2.8 ± 1.06 d	505:516	2.8 ± 1.06 d of age	505:523	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	2	42	theme	age	521:523	arg1	calves					497:502	30 dairy calves	488:502	30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation)	488:551	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	13	43	theme	plasma	1867:1872	arg1	concentrations					1874:1887	plasma concentrations	1867:1887	plasma concentrations	1867:1887	Total urinary recovery (0-24 h relative to marker administration) and plasma concentrations of the markers were unaffected.					
37002139	9	44	theme	enzymes	1434:1440	arg1	chromatography					1309:1322	gas chromatography	1305:1322	gas chromatography	1305:1322	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	9	44	theme	enzymes	1434:1440	arg1	expression					1378:1387	gene expression	1373:1387	gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules	1373:1462	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	9	44	theme	enzymes	1434:1440	arg1	histomorphology					1325:1339	histomorphology	1325:1339	histomorphology by bright-field microscopy	1325:1366	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	6	45	theme	marker	1002:1007	arg1	content					1009:1015	marker content	1002:1015	marker content	1002:1015	Blood and total urine were sequentially collected for 6 and 24 h, respectively, and analyzed for marker content.					
37002139	0	46	from	status	47:52	arg1	response					84:91	response	84:91	response	84:91	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	2	47	theme	standard	533:540	arg1	deviation					542:550	mean ± standard deviation	526:550	mean ± standard deviation	526:550	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	0	48	theme	Gastrointestinal	0:15	arg1	permeability					17:28	Gastrointestinal permeability	0:28	Gastrointestinal permeability	0:28	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	13	49	theme	urinary	1803:1809	arg1	recovery					1811:1818	Total urinary recovery	1797:1818	Total urinary recovery (0-24 h relative to marker administration)	1797:1861	Total urinary recovery (0-24 h relative to marker administration) and plasma concentrations of the markers were unaffected.					
37002139	18	50	theme	serum	2477:2481	arg1	A					2491:2491	serum amyloid A	2477:2491	serum amyloid A	2477:2491	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	18	50	theme	serum	2477:2481	arg1	proteins					2452:2459	the acute-phase proteins	2436:2459	the acute-phase proteins haptoglobin and serum amyloid A postprandially	2436:2506	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	15	51	theme	TNFA	2055:2058	arg1	higher					2073:2078	higher	2073:2078	higher	2073:2078	The expression of TLR4 and TNFA tended to be higher and CD14 was higher in ileum tissue of n-3 calves.					
37002139	15	51	theme	TNFA	2055:2058	arg1	expression					2032:2041	The expression	2028:2041	The expression of TLR4 and TNFA	2028:2058	The expression of TLR4 and TNFA tended to be higher and CD14 was higher in ileum tissue of n-3 calves.					
37002139	17	52	theme	GIT	2261:2263	arg1	permeability					2265:2276	GIT permeability	2261:2276	GIT permeability	2261:2276	Histomorphology and GIT permeability were largely unaffected by treatment.					
37002139	18	53	dep	linseed	2346:2352	arg1	oil					2364:2366	oil	2364:2366	oil	2364:2366	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	9	54	theme	molecules	1454:1462	arg1	chromatography					1309:1322	gas chromatography	1305:1322	gas chromatography	1305:1322	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	9	54	theme	molecules	1454:1462	arg1	expression					1378:1387	gene expression	1373:1387	gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules	1373:1462	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	9	54	theme	molecules	1454:1462	arg1	histomorphology					1325:1339	histomorphology	1325:1339	histomorphology by bright-field microscopy	1325:1366	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	10	55	dep	SAS	1554:1556	arg1	Inc.					1568:1571	Inc.	1568:1571	Inc.	1568:1571	Data were analyzed using PROC GLIMMIX in SAS (version 9.4, SAS Institute Inc.).					
37002139	2	56	theme	mean	526:529	arg1	deviation					542:550	mean ± standard deviation	526:550	mean ± standard deviation	526:550	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	9	57	theme	real-time	1467:1475	arg1	PCR					1490:1492	real-time quantitative PCR	1467:1492	real-time quantitative PCR	1467:1492	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	12	58	theme	proximal	1761:1768	arg1	jejunum					1770:1776	proximal jejunum	1761:1776	proximal jejunum	1761:1776	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	1	59	theme	milk	236:239	arg1	replacer					241:248	milk replacer	236:248	milk replacer (MR)	236:253	The ratio of n-6 to n-3 fatty acid (FA) is between 2 and 10 times higher in milk replacer (MR) than in whole milk, which may promote inflammation and compromise the integrity of the intestinal epithelium.					
37002139	1	59	theme	milk	236:239	arg1	MR					251:252	MR	251:252	MR	251:252	The ratio of n-6 to n-3 fatty acid (FA) is between 2 and 10 times higher in milk replacer (MR) than in whole milk, which may promote inflammation and compromise the integrity of the intestinal epithelium.					
37002139	2	60	theme	crude	683:687	arg1	fat					689:691	crude fat	683:691	n-3; 29.1% crude fat of DM	672:697	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	9	61	theme	junction	1398:1405	arg1	proteins					1407:1414	tight junction proteins	1392:1414	tight junction proteins	1392:1414	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	0	62	theme	preweaning	57:66	arg1	calves					74:79	preweaning dairy calves	57:79	preweaning dairy calves	57:79	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	9	63	theme	Tissue	1247:1252	arg1	samples					1254:1260	Tissue samples	1247:1260	Tissue samples	1247:1260	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	19	64	theme	MR	2621:2622	arg1	composition					2624:2634	MR composition	2621:2634	MR composition	2621:2634	Understanding how MR composition affects dairy calf health may improve nutritional strategies on farm.					
37002139	18	65	theme	fat	2528:2530	arg1	sources					2532:2538	fat sources	2528:2538	fat sources	2528:2538	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	7	66	theme	cytokine	1134:1141	arg1	secretion					1143:1151	cytokine secretion	1134:1151	cytokine secretion from blood cells	1134:1168	Whole blood collected 4 h after the meal was subjected to an ex vivo lipopolysaccharide (LPS) challenge to evaluate cytokine secretion from blood cells.					
37002139	16	67	theme	ex	2204:2205	arg1	challenge					2216:2224	the ex vivo LPS challenge	2200:2224	the ex vivo LPS challenge in n-3 calves	2200:2238	Plasma concentrations of interleukin-4 were decreased in response to the ex vivo LPS challenge in n-3 calves.					
37002139	2	68	theme	40:1	620:623	arg1	ratio					611:615	an n-6:n-3 FA ratio	597:615	an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15)	597:706	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	8	69	theme	tissue	1231:1236	arg1	samples					1238:1244	intestinal tissue samples	1220:1244	intestinal tissue samples	1220:1244	Calves were euthanized on d 25 for collection of intestinal tissue samples.					
37002139	10	70	theme	PROC	1520:1523	arg1	GLIMMIX					1525:1531	PROC GLIMMIX	1520:1531	PROC GLIMMIX in SAS (version 9.4, SAS Institute Inc.)	1520:1572	Data were analyzed using PROC GLIMMIX in SAS (version 9.4, SAS Institute Inc.).					
37002139	12	71	theme	FA	1755:1756	arg1	contents					1708:1715	2-fold higher n-3 FA contents	1687:1715	2-fold higher n-3 FA contents	1687:1715	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	12	71	theme	FA	1755:1756	arg1	ratios					1734:1739	2-fold lower ratios	1721:1739	2-fold lower ratios	1721:1739	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	15	72	theme	n-3	2119:2121	arg1	calves					2123:2128	n-3 calves	2119:2128	n-3 calves	2119:2128	The expression of TLR4 and TNFA tended to be higher and CD14 was higher in ileum tissue of n-3 calves.					
37002139	16	73	theme	LPS	2212:2214	arg1	challenge					2216:2224	the ex vivo LPS challenge	2200:2224	the ex vivo LPS challenge in n-3 calves	2200:2238	Plasma concentrations of interleukin-4 were decreased in response to the ex vivo LPS challenge in n-3 calves.					
37002139	2	74	theme	crude	637:641	arg1	fat					643:645	crude fat	637:645	29.3% crude fat of DM	631:651	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	14	75	theme	n-3	2016:2018	arg1	calves					2020:2025	n-3 calves	2016:2025	n-3 calves	2016:2025	Expression of TJP1 tended to be higher in proximal jejunum tissue and lower in ileum tissue of n-3 calves.					
37002139	12	76	theme	lower	1654:1658	arg1	ratio					1660:1664	a lower ratio	1652:1664	a lower ratio of n-6 to n-3 FA	1652:1681	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	2	77	theme	dairy	491:495	arg1	d					516:516	2.8 ± 1.06 d	505:516	2.8 ± 1.06 d of age	505:523	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	2	77	theme	dairy	491:495	arg1	calves					497:502	30 dairy calves	488:502	30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation)	488:551	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	2	78	dep	%	681:681	arg1	fat					689:691	crude fat	683:691	n-3; 29.1% crude fat of DM	672:697	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	1	79	theme	epithelium	353:362	arg1	integrity					325:333	the integrity	321:333	the integrity of the intestinal epithelium	321:362	The ratio of n-6 to n-3 fatty acid (FA) is between 2 and 10 times higher in milk replacer (MR) than in whole milk, which may promote inflammation and compromise the integrity of the intestinal epithelium.					
37002139	7	80	theme	ex	1079:1080	arg1	challenge					1112:1120	an ex vivo lipopolysaccharide (LPS) challenge	1076:1120	an ex vivo lipopolysaccharide (LPS) challenge	1076:1120	Whole blood collected 4 h after the meal was subjected to an ex vivo lipopolysaccharide (LPS) challenge to evaluate cytokine secretion from blood cells.					
37002139	2	81	theme	n-6	600:602	arg1	ratio					611:615	an n-6:n-3 FA ratio	597:615	an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15)	597:706	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	0	82	from	acid	137:140	arg1	replacer					150:157	milk replacer	145:157	milk replacer	145:157	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	16	83	dep	ex	2204:2205	arg1	vivo					2207:2210	vivo	2207:2210	vivo	2207:2210	Plasma concentrations of interleukin-4 were decreased in response to the ex vivo LPS challenge in n-3 calves.					
37002139	14	84	theme	calves	2020:2025	arg1	tissue					2006:2011	ileum tissue	2000:2011	ileum tissue of n-3 calves	2000:2025	Expression of TJP1 tended to be higher in proximal jejunum tissue and lower in ileum tissue of n-3 calves.					
37002139	2	85	theme	±	509:509	arg1	d					516:516	2.8 ± 1.06 d	505:516	2.8 ± 1.06 d of age	505:523	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	2	85	theme	±	509:509	arg1	calves					497:502	30 dairy calves	488:502	30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation)	488:551	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	2	86	dep	40:1	620:623	arg1	=					656:656	=	656:656	=	656:656	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	1	87	theme	n-6	173:175	arg1	ratio					164:168	The ratio	160:168	The ratio of n-6 to n-3 fatty acid (FA)	160:198	The ratio of n-6 to n-3 fatty acid (FA) is between 2 and 10 times higher in milk replacer (MR) than in whole milk, which may promote inflammation and compromise the integrity of the intestinal epithelium.					
37002139	1	87	theme	n-6	173:175	arg1	higher					226:231	higher	226:231	higher	226:231	The ratio of n-6 to n-3 fatty acid (FA) is between 2 and 10 times higher in milk replacer (MR) than in whole milk, which may promote inflammation and compromise the integrity of the intestinal epithelium.					
37002139	16	88	theme	interleukin-4	2156:2168	arg1	concentrations					2138:2151	Plasma concentrations	2131:2151	Plasma concentrations of interleukin-4	2131:2168	Plasma concentrations of interleukin-4 were decreased in response to the ex vivo LPS challenge in n-3 calves.					
37002139	9	89	theme	bright-field	1344:1355	arg1	microscopy					1357:1366	bright-field microscopy	1344:1366	bright-field microscopy	1344:1366	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	2	90	theme	n-3	604:606	arg1	ratio					611:615	an n-6:n-3 FA ratio	597:615	an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15)	597:706	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	12	91	theme	n-3	1701:1703	arg1	contents					1708:1715	2-fold higher n-3 FA contents	1687:1715	2-fold higher n-3 FA contents	1687:1715	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	0	92	theme	fatty	131:135	arg1	acid					137:140	n-3 fatty acid	127:140	n-3 fatty acid in milk replacer	127:157	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	1	93	theme	n-3	180:182	arg1	FA					196:197	FA	196:197	FA	196:197	The ratio of n-6 to n-3 fatty acid (FA) is between 2 and 10 times higher in milk replacer (MR) than in whole milk, which may promote inflammation and compromise the integrity of the intestinal epithelium.					
37002139	1	93	theme	n-3	180:182	arg1	acid					190:193	n-3 fatty acid	180:193	n-3 fatty acid (FA)	180:198	The ratio of n-6 to n-3 fatty acid (FA) is between 2 and 10 times higher in milk replacer (MR) than in whole milk, which may promote inflammation and compromise the integrity of the intestinal epithelium.					
37002139	2	94	theme	gastrointestinal	427:442	arg1	permeability					450:461	gastrointestinal (GIT) permeability	427:461	gastrointestinal (GIT) permeability	427:461	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	18	95	theme	MR	2586:2587	arg1	formulations					2589:2600	MR formulations	2586:2600	MR formulations	2586:2600	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	18	96	theme	acute-phase	2440:2450	arg1	haptoglobin					2461:2471	haptoglobin	2461:2471	haptoglobin	2461:2471	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	18	96	theme	acute-phase	2440:2450	arg1	A					2491:2491	serum amyloid A	2477:2491	serum amyloid A	2477:2491	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	18	96	theme	acute-phase	2440:2450	arg1	proteins					2452:2459	the acute-phase proteins	2436:2459	the acute-phase proteins haptoglobin and serum amyloid A postprandially	2436:2506	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	9	97	theme	gene	1373:1376	arg1	expression					1378:1387	gene expression	1373:1387	gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules	1373:1462	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	12	98	theme	FA	1680:1681	arg1	ratio					1660:1664	a lower ratio	1652:1664	a lower ratio of n-6 to n-3 FA	1652:1681	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	12	99	contain	had	1683:1685	arg2	ratios					1734:1739	2-fold lower ratios	1721:1739	2-fold lower ratios	1721:1739	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	12	99	contain	had	1683:1685	arg1	Calves					1633:1638	Calves	1633:1638	Calves fed MR with a lower ratio of n-6 to n-3 FA	1633:1681	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	12	99	contain	had	1683:1685	arg2	contents					1708:1715	2-fold higher n-3 FA contents	1687:1715	2-fold higher n-3 FA contents	1687:1715	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	7	100	theme	Whole	1018:1022	arg1	blood					1024:1028	Whole blood	1018:1028	Whole blood collected 4 h after the meal	1018:1057	Whole blood collected 4 h after the meal was subjected to an ex vivo lipopolysaccharide (LPS) challenge to evaluate cytokine secretion from blood cells.					
37002139	16	101	from	challenge	2216:2224	arg1	calves					2233:2238	n-3 calves	2229:2238	n-3 calves	2229:2238	Plasma concentrations of interleukin-4 were decreased in response to the ex vivo LPS challenge in n-3 calves.					
37002139	11	102	theme	fecal	1598:1602	arg1	consistency					1604:1614	fecal consistency	1598:1614	fecal consistency	1598:1614	Growth performance and fecal consistency were unaffected.					
37002139	2	103	theme	n-3	400:402	arg1	ratio					407:411	the n-6:n-3 FA ratio	392:411	the n-6:n-3 FA ratio of MR	392:417	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	9	104	theme	metabolism	1423:1432	arg1	enzymes					1434:1440	lipid metabolism enzymes	1417:1440	lipid metabolism enzymes	1417:1440	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	19	105	from	strategies	2686:2695	arg1	farm					2700:2703	farm	2700:2703	farm	2700:2703	Understanding how MR composition affects dairy calf health may improve nutritional strategies on farm.					
37002139	2	106	dep	d	516:516	arg1	deviation					542:550	mean ± standard deviation	526:550	mean ± standard deviation	526:550	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	2	107	theme	n-6	396:398	arg1	ratio					407:411	the n-6:n-3 FA ratio	392:411	the n-6:n-3 FA ratio of MR	392:417	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	0	108	from	permeability	17:28	arg1	response					84:91	response	84:91	response	84:91	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	1	109	theme	whole	263:267	arg1	milk					269:272	whole milk	263:272	whole milk	263:272	The ratio of n-6 to n-3 fatty acid (FA) is between 2 and 10 times higher in milk replacer (MR) than in whole milk, which may promote inflammation and compromise the integrity of the intestinal epithelium.					
37002139	12	110	from	ratios	1734:1739	arg1	ileum					1782:1786	ileum	1782:1786	ileum	1782:1786	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	12	110	from	ratios	1734:1739	arg1	jejunum					1770:1776	proximal jejunum	1761:1776	proximal jejunum	1761:1776	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	12	111	dep	n-3	1676:1678	arg1	to					1673:1674	to	1673:1674	to	1673:1674	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	2	112	theme	MR	416:417	arg1	ratio					407:411	the n-6:n-3 FA ratio	392:411	the n-6:n-3 FA ratio of MR	392:417	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	2	113	theme	GIT	445:447	arg1	permeability					450:461	gastrointestinal (GIT) permeability	427:461	gastrointestinal (GIT) permeability	427:461	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	19	114	dep	Understanding	2603:2615	arg1	affects					2636:2642	affects	2636:2642	affects dairy calf health	2636:2660	Understanding how MR composition affects dairy calf health may improve nutritional strategies on farm.					
37002139	2	115	theme	DM	650:651	arg1	fat					643:645	crude fat	637:645	29.3% crude fat of DM	631:651	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	18	116	theme	greater	2410:2416	arg1	concentrations					2418:2431	greater concentrations	2410:2431	greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially	2410:2506	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	12	117	from	contents	1708:1715	arg1	ileum					1782:1786	ileum	1782:1786	ileum	1782:1786	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	12	117	from	contents	1708:1715	arg1	jejunum					1770:1776	proximal jejunum	1761:1776	proximal jejunum	1761:1776	Calves fed MR with a lower ratio of n-6 to n-3 FA had 2-fold higher n-3 FA contents and 2-fold lower ratios of n-6 to n-3 FA in proximal jejunum and ileum tissues.					
37002139	19	118	theme	nutritional	2674:2684	arg1	strategies					2686:2695	nutritional strategies	2674:2695	nutritional strategies on farm	2674:2703	Understanding how MR composition affects dairy calf health may improve nutritional strategies on farm.					
37002139	9	119	theme	immune	1447:1452	arg1	molecules					1454:1462	immune molecules	1447:1462	immune molecules	1447:1462	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	2	120	theme	±	531:531	arg1	deviation					542:550	mean ± standard deviation	526:550	mean ± standard deviation	526:550	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	0	121	theme	inflammatory	34:45	arg1	status					47:52	inflammatory status	34:52	inflammatory status	34:52	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	2	122	theme	6.5:1	665:669	arg1	ratio					611:615	an n-6:n-3 FA ratio	597:615	an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15)	597:706	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	2	123	dep	=	656:656	arg1	CON					626:628	CON	626:628	CON	626:628	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	2	123	dep	=	656:656	arg1	%					635:635	29.3%	631:635	29.3% crude fat of DM	631:651	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	9	124	theme	quantitative	1477:1488	arg1	PCR					1490:1492	real-time quantitative PCR	1467:1492	real-time quantitative PCR	1467:1492	Tissue samples were processed to assess FA composition by gas chromatography, histomorphology by bright-field microscopy, and gene expression of tight junction proteins, lipid metabolism enzymes, and immune molecules by real-time quantitative PCR.					
37002139	10	125	dep	version	1541:1547	arg1	SAS					1554:1556	SAS	1554:1556	SAS Institute Inc.	1554:1571	Data were analyzed using PROC GLIMMIX in SAS (version 9.4, SAS Institute Inc.).					
37002139	18	126	theme	algae	2358:2362	arg1	inclusion					2333:2341	the inclusion	2329:2341	the inclusion of linseed and algae oil	2329:2366	Furthermore, the inclusion of linseed and algae oil may promote inflammation, as suggested by greater concentrations of the acute-phase proteins haptoglobin and serum amyloid A postprandially, demonstrating that fat sources should be evaluated for their suitability for MR formulations.					
37002139	0	127	theme	dairy	68:72	arg1	calves					74:79	preweaning dairy calves	57:79	preweaning dairy calves	57:79	Gastrointestinal permeability and inflammatory status of preweaning dairy calves in response to decreasing the ratio of n-6 to n-3 fatty acid in milk replacer.					
37002139	2	128	theme	FA	404:405	arg1	ratio					407:411	the n-6:n-3 FA ratio	392:411	the n-6:n-3 FA ratio of MR	392:417	To evaluate how decreasing the n-6:n-3 FA ratio of MR affects gastrointestinal (GIT) permeability and inflammatory status, 30 dairy calves (2.8 ± 1.06 d of age; mean ± standard deviation) were randomly assigned to be fed an MR with an n-6:n-3 FA ratio of 40:1 (CON; 29.3% crude fat of DM; n = 15) or 6.5:1 (n-3; 29.1% crude fat of DM; n = 15).					
37002139	8	129	theme	intestinal	1220:1229	arg1	samples					1238:1244	intestinal tissue samples	1220:1244	intestinal tissue samples	1220:1244	Calves were euthanized on d 25 for collection of intestinal tissue samples.					
37002139	14	130	theme	ileum	2000:2004	arg1	tissue					2006:2011	ileum tissue	2000:2011	ileum tissue of n-3 calves	2000:2025	Expression of TJP1 tended to be higher in proximal jejunum tissue and lower in ileum tissue of n-3 calves.					
37002139	15	131	theme	calves	2123:2128	arg1	tissue					2109:2114	ileum tissue	2103:2114	ileum tissue of n-3 calves	2103:2128	The expression of TLR4 and TNFA tended to be higher and CD14 was higher in ileum tissue of n-3 calves.					
37002139	13	132	theme	Total	1797:1801	arg1	recovery					1811:1818	Total urinary recovery	1797:1818	Total urinary recovery (0-24 h relative to marker administration)	1797:1861	Total urinary recovery (0-24 h relative to marker administration) and plasma concentrations of the markers were unaffected.					
37002139	7	133	theme	blood	1158:1162	arg1	cells					1164:1168	blood cells	1158:1168	blood cells	1158:1168	Whole blood collected 4 h after the meal was subjected to an ex vivo lipopolysaccharide (LPS) challenge to evaluate cytokine secretion from blood cells.					
37002139	8	134	theme	samples	1238:1244	arg1	collection					1206:1215	collection	1206:1215	collection of intestinal tissue samples	1206:1244	Calves were euthanized on d 25 for collection of intestinal tissue samples.					
37002139	15	135	theme	TLR4	2046:2049	arg1	higher					2073:2078	higher	2073:2078	higher	2073:2078	The expression of TLR4 and TNFA tended to be higher and CD14 was higher in ileum tissue of n-3 calves.					
37002139	15	135	theme	TLR4	2046:2049	arg1	expression					2032:2041	The expression	2028:2041	The expression of TLR4 and TNFA	2028:2058	The expression of TLR4 and TNFA tended to be higher and CD14 was higher in ileum tissue of n-3 calves.					
37002139	13	136	theme	markers	1896:1902	arg1	recovery					1811:1818	Total urinary recovery	1797:1818	Total urinary recovery (0-24 h relative to marker administration)	1797:1861	Total urinary recovery (0-24 h relative to marker administration) and plasma concentrations of the markers were unaffected.					
37002139	13	136	theme	markers	1896:1902	arg1	concentrations					1874:1887	plasma concentrations	1867:1887	plasma concentrations	1867:1887	Total urinary recovery (0-24 h relative to marker administration) and plasma concentrations of the markers were unaffected.					
37002139	4	137	theme	fecal	769:773	arg1	consistency					775:785	fecal consistency	769:785	fecal consistency	769:785	Calves were weighed and fecal consistency was analyzed weekly.					
37002139	10	138	dep	SAS	1536:1538	arg1	version					1541:1547	version 9.4	1541:1551	version 9.4	1541:1551	Data were analyzed using PROC GLIMMIX in SAS (version 9.4, SAS Institute Inc.).					
37002139	19	139	theme	dairy	2644:2648	arg1	health					2655:2660	dairy calf health	2644:2660	dairy calf health	2644:2660	Understanding how MR composition affects dairy calf health may improve nutritional strategies on farm.					
37002139	15	140	theme	ileum	2103:2107	arg1	tissue					2109:2114	ileum tissue	2103:2114	ileum tissue of n-3 calves	2103:2128	The expression of TLR4 and TNFA tended to be higher and CD14 was higher in ileum tissue of n-3 calves.					
35271579	8	0	theme	intestinal	1274:1283	arg1	microbiome					1285:1294	the intestinal microbiome	1270:1294	the intestinal microbiome producing SCFAs	1270:1310	Many studies have proposed that the mechanism of dietary fiber regulating asthma may involve the intestinal microbiome producing SCFAs, but the current research shows that a high-cellulose diet cannot increase the content of SCFAs in the intestine.					
35271579	6	1	theme	Romboutsla	1029:1038	arg1	_torques_group					1055:1068	the unique genus Romboutsla, [Ruminococcus]_torques_group	1012:1068	_torques_group	1055:1068	At the family level, a new dominant fungus family Peptostreptococcaceae is produced, and at the genus level, the unique genus Romboutsla, [Ruminococcus]_torques_group was generated.					
35271579	6	2	theme	family	946:951	arg1	Peptostreptococcaceae					953:973	a new dominant fungus family Peptostreptococcaceae	924:973	a new dominant fungus family Peptostreptococcaceae	924:973	At the family level, a new dominant fungus family Peptostreptococcaceae is produced, and at the genus level, the unique genus Romboutsla, [Ruminococcus]_torques_group was generated.					
35271579	8	3	from	content	1391:1397	arg1	intestine					1415:1423	the intestine	1411:1423	the intestine	1411:1423	Many studies have proposed that the mechanism of dietary fiber regulating asthma may involve the intestinal microbiome producing SCFAs, but the current research shows that a high-cellulose diet cannot increase the content of SCFAs in the intestine.					
35271579	3	4	theme	intestinal	640:649	arg1	microbiome					651:660	the intestinal microbiome	636:660	the intestinal microbiome	636:660	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	7	5	dep	genera	1091:1096	arg1	These					1085:1089	These	1085:1089	These	1085:1089	These genera and families of bacteria are closely correlated with lipid metabolism in vivo.					
35271579	1	6	theme	high	162:165	arg1	intake					181:186	a high dietary fiber intake	160:186	a high dietary fiber intake	160:186	Numerous epidemiological studies have shown that a high dietary fiber intake is associated inversely with the incidence of asthma in the population.					
35271579	3	7	from	outcomes	505:512	arg1	model					550:554	a mouse model	542:554	a mouse model of asthma	542:564	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	6	8	theme	fungus	939:944	arg1	Peptostreptococcaceae					953:973	a new dominant fungus family Peptostreptococcaceae	924:973	a new dominant fungus family Peptostreptococcaceae	924:973	At the family level, a new dominant fungus family Peptostreptococcaceae is produced, and at the genus level, the unique genus Romboutsla, [Ruminococcus]_torques_group was generated.					
35271579	0	9	theme	mouse	98:102	arg1	model					104:108	a mouse model	96:108	a mouse model	96:108	High cellulose dietary intake relieves asthma inflammation through the intestinal microbiome in a mouse model.					
35271579	8	10	theme	current	1321:1327	arg1	research					1329:1336	the current research	1317:1336	the current research	1317:1336	Many studies have proposed that the mechanism of dietary fiber regulating asthma may involve the intestinal microbiome producing SCFAs, but the current research shows that a high-cellulose diet cannot increase the content of SCFAs in the intestine.					
35271579	6	11	theme	dominant	930:937	arg1	Peptostreptococcaceae					953:973	a new dominant fungus family Peptostreptococcaceae	924:973	a new dominant fungus family Peptostreptococcaceae	924:973	At the family level, a new dominant fungus family Peptostreptococcaceae is produced, and at the genus level, the unique genus Romboutsla, [Ruminococcus]_torques_group was generated.					
35271579	5	12	theme	microbiome	891:900	arg1	composition					861:871	the composition	857:871	the composition of the intestinal microbiome	857:900	Furthermore, it dramatically changes the composition of the intestinal microbiome.					
35271579	3	13	theme	intestinal	678:687	arg1	SCFAs					714:718	SCFAs	714:718	SCFAs	714:718	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	3	13	theme	intestinal	678:687	arg1	acids					707:711	intestinal short-chain fatty acids	678:711	intestinal short-chain fatty acids (SCFAs)	678:719	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	2	14	theme	many	276:279	arg1	studies					281:287	many studies	276:287	many studies on the role of soluble dietary fiber	276:324	There have been many studies on the role of soluble dietary fiber, but the mechanism of action for insoluble dietary fiber, such as cellulose-the most widely existing dietary fiber, in asthma is still unclear.					
35271579	5	15	theme	intestinal	880:889	arg1	microbiome					891:900	the intestinal microbiome	876:900	the intestinal microbiome	876:900	Furthermore, it dramatically changes the composition of the intestinal microbiome.					
35271579	3	16	theme	short-chain	689:699	arg1	SCFAs					714:718	SCFAs	714:718	SCFAs	714:718	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	3	16	theme	short-chain	689:699	arg1	acids					707:711	intestinal short-chain fatty acids	678:711	intestinal short-chain fatty acids (SCFAs)	678:719	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	9	17	theme	SCFAs	1612:1616	arg1	independent					1597:1607	independent	1597:1607	independent	1597:1607	These data suggest that a high-cellulose diet decreases asthma symptoms by altering the composition of the intestinal microbiome, however, this mechanism is thought to be independent of SCFAs and may involve the regulation of lipid metabolism.					
35271579	6	18	theme	unique	1016:1021	arg1	_torques_group					1055:1068	the unique genus Romboutsla, [Ruminococcus]_torques_group	1012:1068	_torques_group	1055:1068	At the family level, a new dominant fungus family Peptostreptococcaceae is produced, and at the genus level, the unique genus Romboutsla, [Ruminococcus]_torques_group was generated.					
35271579	9	19	theme	intestinal	1533:1542	arg1	microbiome					1544:1553	the intestinal microbiome	1529:1553	the intestinal microbiome	1529:1553	These data suggest that a high-cellulose diet decreases asthma symptoms by altering the composition of the intestinal microbiome, however, this mechanism is thought to be independent of SCFAs and may involve the regulation of lipid metabolism.					
35271579	1	20	theme	dietary	167:173	arg1	intake					181:186	a high dietary fiber intake	160:186	a high dietary fiber intake	160:186	Numerous epidemiological studies have shown that a high dietary fiber intake is associated inversely with the incidence of asthma in the population.					
35271579	6	21	theme	new	926:928	arg1	Peptostreptococcaceae					953:973	a new dominant fungus family Peptostreptococcaceae	924:973	a new dominant fungus family Peptostreptococcaceae	924:973	At the family level, a new dominant fungus family Peptostreptococcaceae is produced, and at the genus level, the unique genus Romboutsla, [Ruminococcus]_torques_group was generated.					
35271579	4	22	from	symptoms	792:799	arg1	mice					814:817	asthmatic mice	804:817	asthmatic mice	804:817	A high-cellulose diet can reduce lung inflammation and asthma symptoms in asthmatic mice.					
35271579	4	23	theme	asthma	785:790	arg1	symptoms					792:799	asthma symptoms	785:799	asthma symptoms	785:799	A high-cellulose diet can reduce lung inflammation and asthma symptoms in asthmatic mice.					
35271579	6	24	theme	genus	1023:1027	arg1	_torques_group					1055:1068	the unique genus Romboutsla, [Ruminococcus]_torques_group	1012:1068	_torques_group	1055:1068	At the family level, a new dominant fungus family Peptostreptococcaceae is produced, and at the genus level, the unique genus Romboutsla, [Ruminococcus]_torques_group was generated.					
35271579	2	25	theme	insoluble	359:367	arg1	fiber					377:381	insoluble dietary fiber	359:381	insoluble dietary fiber	359:381	There have been many studies on the role of soluble dietary fiber, but the mechanism of action for insoluble dietary fiber, such as cellulose-the most widely existing dietary fiber, in asthma is still unclear.					
35271579	0	26	theme	cellulose	5:13	arg1	intake					23:28	High cellulose dietary intake	0:28	High cellulose dietary intake	0:28	High cellulose dietary intake relieves asthma inflammation through the intestinal microbiome in a mouse model.					
35271579	6	27	theme	family	910:915	arg1	level					917:921	the family level	906:921	the family level	906:921	At the family level, a new dominant fungus family Peptostreptococcaceae is produced, and at the genus level, the unique genus Romboutsla, [Ruminococcus]_torques_group was generated.					
35271579	4	28	theme	asthmatic	804:812	arg1	mice					814:817	asthmatic mice	804:817	asthmatic mice	804:817	A high-cellulose diet can reduce lung inflammation and asthma symptoms in asthmatic mice.					
35271579	3	29	theme	diet	534:537	arg1	outcomes					505:512	the outcomes	501:512	the outcomes of a high-cellulose diet in a mouse model of asthma	501:564	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	7	30	theme	bacteria	1114:1121	arg1	genera					1091:1096	genera	1091:1096	genera	1091:1096	These genera and families of bacteria are closely correlated with lipid metabolism in vivo.					
35271579	7	30	theme	bacteria	1114:1121	arg1	families					1102:1109	families	1102:1109	families	1102:1109	These genera and families of bacteria are closely correlated with lipid metabolism in vivo.					
35271579	0	31	theme	High	0:3	arg1	intake					23:28	High cellulose dietary intake	0:28	High cellulose dietary intake	0:28	High cellulose dietary intake relieves asthma inflammation through the intestinal microbiome in a mouse model.					
35271579	2	32	theme	action	348:353	arg1	unclear					461:467	unclear	461:467	unclear	461:467	There have been many studies on the role of soluble dietary fiber, but the mechanism of action for insoluble dietary fiber, such as cellulose-the most widely existing dietary fiber, in asthma is still unclear.					
35271579	2	32	theme	action	348:353	arg1	mechanism					335:343	the mechanism	331:343	the mechanism of action for insoluble dietary fiber, such as cellulose-the most widely existing dietary fiber, in asthma	331:450	There have been many studies on the role of soluble dietary fiber, but the mechanism of action for insoluble dietary fiber, such as cellulose-the most widely existing dietary fiber, in asthma is still unclear.					
35271579	2	33	from	mechanism	335:343	arg1	asthma					445:450	asthma	445:450	asthma	445:450	There have been many studies on the role of soluble dietary fiber, but the mechanism of action for insoluble dietary fiber, such as cellulose-the most widely existing dietary fiber, in asthma is still unclear.					
35271579	9	34	theme	microbiome	1544:1553	arg1	composition					1514:1524	the composition	1510:1524	the composition of the intestinal microbiome	1510:1553	These data suggest that a high-cellulose diet decreases asthma symptoms by altering the composition of the intestinal microbiome, however, this mechanism is thought to be independent of SCFAs and may involve the regulation of lipid metabolism.					
35271579	6	35	theme	genus	999:1003	arg1	level					1005:1009	the genus level	995:1009	the genus level	995:1009	At the family level, a new dominant fungus family Peptostreptococcaceae is produced, and at the genus level, the unique genus Romboutsla, [Ruminococcus]_torques_group was generated.					
35271579	0	36	theme	dietary	15:21	arg1	intake					23:28	High cellulose dietary intake	0:28	High cellulose dietary intake	0:28	High cellulose dietary intake relieves asthma inflammation through the intestinal microbiome in a mouse model.					
35271579	3	37	theme	fatty	701:705	arg1	SCFAs					714:718	SCFAs	714:718	SCFAs	714:718	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	3	37	theme	fatty	701:705	arg1	acids					707:711	intestinal short-chain fatty acids	678:711	intestinal short-chain fatty acids (SCFAs)	678:719	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	3	38	theme	high-cellulose	519:532	arg1	diet					534:537	a high-cellulose diet	517:537	a high-cellulose diet	517:537	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	9	39	theme	asthma	1482:1487	arg1	symptoms					1489:1496	asthma symptoms	1482:1496	asthma symptoms	1482:1496	These data suggest that a high-cellulose diet decreases asthma symptoms by altering the composition of the intestinal microbiome, however, this mechanism is thought to be independent of SCFAs and may involve the regulation of lipid metabolism.					
35271579	9	40	theme	high-cellulose	1452:1465	arg1	diet					1467:1470	a high-cellulose diet	1450:1470	a high-cellulose diet	1450:1470	These data suggest that a high-cellulose diet decreases asthma symptoms by altering the composition of the intestinal microbiome, however, this mechanism is thought to be independent of SCFAs and may involve the regulation of lipid metabolism.					
35271579	0	41	theme	asthma	39:44	arg1	inflammation					46:57	asthma inflammation	39:57	asthma inflammation	39:57	High cellulose dietary intake relieves asthma inflammation through the intestinal microbiome in a mouse model.					
35271579	2	42	theme	fiber	320:324	arg1	role					296:299	the role	292:299	the role of soluble dietary fiber	292:324	There have been many studies on the role of soluble dietary fiber, but the mechanism of action for insoluble dietary fiber, such as cellulose-the most widely existing dietary fiber, in asthma is still unclear.					
35271579	0	43	from	microbiome	82:91	arg1	model					104:108	a mouse model	96:108	a mouse model	96:108	High cellulose dietary intake relieves asthma inflammation through the intestinal microbiome in a mouse model.					
35271579	4	44	theme	lung	763:766	arg1	inflammation					768:779	lung inflammation	763:779	lung inflammation	763:779	A high-cellulose diet can reduce lung inflammation and asthma symptoms in asthmatic mice.					
35271579	2	45	theme	dietary	369:375	arg1	fiber					377:381	insoluble dietary fiber	359:381	insoluble dietary fiber	359:381	There have been many studies on the role of soluble dietary fiber, but the mechanism of action for insoluble dietary fiber, such as cellulose-the most widely existing dietary fiber, in asthma is still unclear.					
35271579	9	46	theme	lipid	1652:1656	arg1	metabolism					1658:1667	lipid metabolism	1652:1667	lipid metabolism	1652:1667	These data suggest that a high-cellulose diet decreases asthma symptoms by altering the composition of the intestinal microbiome, however, this mechanism is thought to be independent of SCFAs and may involve the regulation of lipid metabolism.					
35271579	8	47	theme	regulating	1240:1249	arg1	asthma					1251:1256	dietary fiber regulating asthma	1226:1256	dietary fiber regulating asthma	1226:1256	Many studies have proposed that the mechanism of dietary fiber regulating asthma may involve the intestinal microbiome producing SCFAs, but the current research shows that a high-cellulose diet cannot increase the content of SCFAs in the intestine.					
35271579	8	48	theme	fiber	1234:1238	arg1	asthma					1251:1256	dietary fiber regulating asthma	1226:1256	dietary fiber regulating asthma	1226:1256	Many studies have proposed that the mechanism of dietary fiber regulating asthma may involve the intestinal microbiome producing SCFAs, but the current research shows that a high-cellulose diet cannot increase the content of SCFAs in the intestine.					
35271579	3	49	from	manifestations	592:605	arg1	SCFAs					714:718	SCFAs	714:718	SCFAs	714:718	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	3	49	from	manifestations	592:605	arg1	acids					707:711	intestinal short-chain fatty acids	678:711	intestinal short-chain fatty acids (SCFAs)	678:719	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	3	49	from	manifestations	592:605	arg1	microbiome					651:660	the intestinal microbiome	636:660	the intestinal microbiome	636:660	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	9	50	theme	metabolism	1658:1667	arg1	regulation					1638:1647	the regulation	1634:1647	the regulation of lipid metabolism	1634:1667	These data suggest that a high-cellulose diet decreases asthma symptoms by altering the composition of the intestinal microbiome, however, this mechanism is thought to be independent of SCFAs and may involve the regulation of lipid metabolism.					
35271579	8	51	theme	SCFAs	1402:1406	arg1	content					1391:1397	the content	1387:1397	the content of SCFAs in the intestine	1387:1423	Many studies have proposed that the mechanism of dietary fiber regulating asthma may involve the intestinal microbiome producing SCFAs, but the current research shows that a high-cellulose diet cannot increase the content of SCFAs in the intestine.					
35271579	8	52	theme	high-cellulose	1351:1364	arg1	diet					1366:1369	a high-cellulose diet	1349:1369	a high-cellulose diet	1349:1369	Many studies have proposed that the mechanism of dietary fiber regulating asthma may involve the intestinal microbiome producing SCFAs, but the current research shows that a high-cellulose diet cannot increase the content of SCFAs in the intestine.					
35271579	4	53	from	inflammation	768:779	arg1	mice					814:817	asthmatic mice	804:817	asthmatic mice	804:817	A high-cellulose diet can reduce lung inflammation and asthma symptoms in asthmatic mice.					
35271579	1	54	theme	Numerous	111:118	arg1	studies					136:142	Numerous epidemiological studies	111:142	Numerous epidemiological studies	111:142	Numerous epidemiological studies have shown that a high dietary fiber intake is associated inversely with the incidence of asthma in the population.					
35271579	2	55	theme	dietary	427:433	arg1	fiber					435:439	dietary fiber	427:439	dietary fiber	427:439	There have been many studies on the role of soluble dietary fiber, but the mechanism of action for insoluble dietary fiber, such as cellulose-the most widely existing dietary fiber, in asthma is still unclear.					
35271579	3	56	theme	mouse	544:548	arg1	model					550:554	a mouse model	542:554	a mouse model of asthma	542:564	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	1	57	theme	epidemiological	120:134	arg1	studies					136:142	Numerous epidemiological studies	111:142	Numerous epidemiological studies	111:142	Numerous epidemiological studies have shown that a high dietary fiber intake is associated inversely with the incidence of asthma in the population.					
35271579	1	58	from	incidence	221:229	arg1	population					248:257	the population	244:257	the population	244:257	Numerous epidemiological studies have shown that a high dietary fiber intake is associated inversely with the incidence of asthma in the population.					
35271579	6	59	dep	_torques_group	1055:1068	arg1	[Ruminococcus					1041:1053	the unique genus Romboutsla, [Ruminococcus]_torques_group	1012:1068	[Ruminococcus	1041:1053	At the family level, a new dominant fungus family Peptostreptococcaceae is produced, and at the genus level, the unique genus Romboutsla, [Ruminococcus]_torques_group was generated.					
35271579	4	60	theme	high-cellulose	732:745	arg1	diet					747:750	A high-cellulose diet	730:750	A high-cellulose diet	730:750	A high-cellulose diet can reduce lung inflammation and asthma symptoms in asthmatic mice.					
35271579	2	61	theme	dietary	312:318	arg1	fiber					320:324	soluble dietary fiber	304:324	soluble dietary fiber	304:324	There have been many studies on the role of soluble dietary fiber, but the mechanism of action for insoluble dietary fiber, such as cellulose-the most widely existing dietary fiber, in asthma is still unclear.					
35271579	3	62	theme	pathological	579:590	arg1	manifestations					592:605	pathological manifestations	579:605	pathological manifestations within the lungs	579:622	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	2	63	theme	soluble	304:310	arg1	fiber					320:324	soluble dietary fiber	304:324	soluble dietary fiber	304:324	There have been many studies on the role of soluble dietary fiber, but the mechanism of action for insoluble dietary fiber, such as cellulose-the most widely existing dietary fiber, in asthma is still unclear.					
35271579	2	64	from	studies	281:287	arg1	role					296:299	the role	292:299	the role of soluble dietary fiber	292:324	There have been many studies on the role of soluble dietary fiber, but the mechanism of action for insoluble dietary fiber, such as cellulose-the most widely existing dietary fiber, in asthma is still unclear.					
35271579	8	65	theme	asthma	1251:1256	arg1	mechanism					1213:1221	the mechanism	1209:1221	the mechanism of dietary fiber regulating asthma	1209:1256	Many studies have proposed that the mechanism of dietary fiber regulating asthma may involve the intestinal microbiome producing SCFAs, but the current research shows that a high-cellulose diet cannot increase the content of SCFAs in the intestine.					
35271579	0	66	theme	intestinal	71:80	arg1	microbiome					82:91	the intestinal microbiome	67:91	the intestinal microbiome in a mouse model	67:108	High cellulose dietary intake relieves asthma inflammation through the intestinal microbiome in a mouse model.					
35271579	3	67	theme	asthma	559:564	arg1	model					550:554	a mouse model	542:554	a mouse model of asthma	542:564	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	3	68	theme	current	474:480	arg1	study					482:486	The current study	470:486	The current study	470:486	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	1	69	theme	fiber	175:179	arg1	intake					181:186	a high dietary fiber intake	160:186	a high dietary fiber intake	160:186	Numerous epidemiological studies have shown that a high dietary fiber intake is associated inversely with the incidence of asthma in the population.					
35271579	1	70	theme	asthma	234:239	arg1	incidence					221:229	the incidence	217:229	the incidence of asthma in the population	217:257	Numerous epidemiological studies have shown that a high dietary fiber intake is associated inversely with the incidence of asthma in the population.					
35271579	3	71	from	changes	625:631	arg1	SCFAs					714:718	SCFAs	714:718	SCFAs	714:718	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	3	71	from	changes	625:631	arg1	acids					707:711	intestinal short-chain fatty acids	678:711	intestinal short-chain fatty acids (SCFAs)	678:719	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	3	71	from	changes	625:631	arg1	microbiome					651:660	the intestinal microbiome	636:660	the intestinal microbiome	636:660	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	8	72	theme	Many	1177:1180	arg1	studies					1182:1188	Many studies	1177:1188	Many studies	1177:1188	Many studies have proposed that the mechanism of dietary fiber regulating asthma may involve the intestinal microbiome producing SCFAs, but the current research shows that a high-cellulose diet cannot increase the content of SCFAs in the intestine.					
35271579	3	73	from	changes	667:673	arg1	SCFAs					714:718	SCFAs	714:718	SCFAs	714:718	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	3	73	from	changes	667:673	arg1	acids					707:711	intestinal short-chain fatty acids	678:711	intestinal short-chain fatty acids (SCFAs)	678:719	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	3	73	from	changes	667:673	arg1	microbiome					651:660	the intestinal microbiome	636:660	the intestinal microbiome	636:660	The current study investigated the outcomes of a high-cellulose diet in a mouse model of asthma and detected pathological manifestations within the lungs, changes in the intestinal microbiome, and changes in intestinal short-chain fatty acids (SCFAs) in mice.					
35271579	8	74	theme	dietary	1226:1232	arg1	asthma					1251:1256	dietary fiber regulating asthma	1226:1256	dietary fiber regulating asthma	1226:1256	Many studies have proposed that the mechanism of dietary fiber regulating asthma may involve the intestinal microbiome producing SCFAs, but the current research shows that a high-cellulose diet cannot increase the content of SCFAs in the intestine.					
35271579	7	75	theme	lipid	1151:1155	arg1	metabolism					1157:1166	lipid metabolism	1151:1166	lipid metabolism	1151:1166	These genera and families of bacteria are closely correlated with lipid metabolism in vivo.					
35880540	2	0	theme	Schiff	521:526	arg1	reaction					533:540	a simple one-step Schiff base reaction	503:540	a simple one-step Schiff base reaction	503:540	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	4	1	from	plasma	935:940	arg1	extract					909:915	extract	909:915	extract	909:915	The as-prepared material can quickly extract angoroside C from plasma with high extraction efficiency, be easily separated with a magnet afterward, and can be reused for at least five times.					
35880540	0	2	theme	Granule	206:212	arg1	administration					169:182	oral administration	164:182	oral administration of Xuanbo Shuangsheng Granule	164:212	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	2	3	theme	trace	585:589	arg1	C					602:602	trace angoroside C	585:602	trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection	585:705	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	6	4	theme	C	1382:1382	arg1	study					1356:1360	the pharmacokinetic study	1336:1360	the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule	1336:1446	Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.					
35880540	3	5	theme	strong	854:859	arg1	stability					861:869	strong stability	854:869	strong stability	854:869	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	2	6	from	C	602:602	arg1	plasma					611:616	rat plasma	607:616	rat plasma	607:616	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	0	7	theme	pharmacokinetic	100:114	arg1	study					116:120	pharmacokinetic study	100:120	pharmacokinetic study	100:120	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	3	8	theme	high	766:769	arg1	responsiveness					780:793	high magnetic responsiveness	766:793	high magnetic responsiveness (35.67 emu·g-1 )	766:810	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	3	8	theme	high	766:769	arg1	emu·g-1					802:808	35.67 emu·g-1	796:808	35.67 emu·g-1	796:808	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	0	9	theme	Shuangsheng	194:204	arg1	Granule					206:212	Xuanbo Shuangsheng Granule	187:212	Xuanbo Shuangsheng Granule	187:212	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	5	10	theme	low	1156:1158	arg1	0.1 ng·ml-1					1185:1195	0.1 ng·ml-1	1185:1195	0.1 ng·ml-1	1185:1195	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	10	theme	low	1156:1158	arg1	limit					1160:1164	low limit	1156:1164	low limit of quantification (0.1 ng·ml-1 )	1156:1197	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	0	11	from	study	116:120	arg1	plasma					151:156	rat plasma	147:156	rat plasma	147:156	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	0	12	theme	trace	125:129	arg1	C					142:142	trace angoroside C	125:142	trace angoroside C in rat plasma	125:156	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	3	13	theme	magnetic	771:778	arg1	responsiveness					780:793	high magnetic responsiveness	766:793	high magnetic responsiveness (35.67 emu·g-1 )	766:810	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	3	13	theme	magnetic	771:778	arg1	emu·g-1					802:808	35.67 emu·g-1	796:808	35.67 emu·g-1	796:808	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	6	14	theme	Granule	1440:1446	arg1	administration					1403:1416	oral administration	1398:1416	oral administration of Xuanbo Shuangsheng Granule	1398:1446	Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.					
35880540	6	15	theme	angoroside	1371:1380	arg1	C					1382:1382	trace angoroside C	1365:1382	trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule	1365:1446	Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.					
35880540	5	16	theme	relative	1262:1269	arg1	deviation					1280:1288	percentage relative standard deviation 3.60-10.90%	1251:1300	percentage relative standard deviation 3.60-10.90%	1251:1300	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	16	theme	relative	1262:1269	arg1	precision					1240:1248	good precision	1235:1248	good precision (percentage relative standard deviation 3.60-10.90%)	1235:1301	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	17	theme	good	1200:1203	arg1	accuracy					1205:1212	good accuracy	1200:1212	good accuracy (93.18-105.36%)	1200:1228	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	17	theme	good	1200:1203	arg1	%					1227:1227	93.18-105.36%	1215:1227	93.18-105.36%	1215:1227	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	18	dep	deviation	1280:1288	arg1	%					1300:1300	3.60-10.90%	1290:1300	percentage relative standard deviation 3.60-10.90%	1251:1300	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	6	19	used	used	1328:1331	arg2	method					1317:1322	the method	1313:1322	the method	1313:1322	Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.					
35880540	5	20	theme	good	1235:1238	arg1	deviation					1280:1288	percentage relative standard deviation 3.60-10.90%	1251:1300	percentage relative standard deviation 3.60-10.90%	1251:1300	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	20	theme	good	1235:1238	arg1	precision					1240:1248	good precision	1235:1248	good precision (percentage relative standard deviation 3.60-10.90%)	1235:1301	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	0	21	theme	C	142:142	arg1	determination					82:94	determination	82:94	determination	82:94	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	0	21	theme	C	142:142	arg1	study					116:120	pharmacokinetic study	100:120	pharmacokinetic study	100:120	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	2	22	theme	spectrometry	684:695	arg1	detection					697:705	ultra-high-performance liquid chromatography-tandem mass spectrometry detection	627:705	ultra-high-performance liquid chromatography-tandem mass spectrometry detection	627:705	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	0	23	theme	angoroside	131:140	arg1	C					142:142	trace angoroside C	125:142	trace angoroside C in rat plasma	125:156	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	2	24	theme	one-step	512:519	arg1	reaction					533:540	a simple one-step Schiff base reaction	503:540	a simple one-step Schiff base reaction	503:540	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	2	25	theme	mass	679:682	arg1	spectrometry					684:695	ultra-high-performance liquid chromatography-tandem mass spectrometry	627:695	ultra-high-performance liquid chromatography-tandem mass spectrometry detection	627:705	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	6	26	from	study	1356:1360	arg1	rats					1387:1390	rats	1387:1390	rats after oral administration of Xuanbo Shuangsheng Granule	1387:1446	Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.					
35880540	1	27	theme	medicine	258:265	arg1	preparation					276:286	the traditional Chinese medicine compound preparation	234:286	the traditional Chinese medicine compound preparation	234:286	The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection.					
35880540	6	28	theme	Shuangsheng	1428:1438	arg1	Granule					1440:1446	Xuanbo Shuangsheng Granule	1421:1446	Xuanbo Shuangsheng Granule	1421:1446	Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.					
35880540	6	29	theme	trace	1365:1369	arg1	C					1382:1382	trace angoroside C	1365:1382	trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule	1365:1446	Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.					
35880540	0	30	theme	covalent-organic	9:24	arg1	extraction					43:52	Magnetic covalent-organic frameworks-based extraction	0:52	Magnetic covalent-organic frameworks-based extraction	0:52	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	1	31	theme	plasma	395:400	arg1	detection					416:424	the plasma concentration detection	391:424	the plasma concentration detection	391:424	The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection.					
35880540	2	32	theme	chromatography-tandem	657:677	arg1	spectrometry					684:695	ultra-high-performance liquid chromatography-tandem mass spectrometry	627:695	ultra-high-performance liquid chromatography-tandem mass spectrometry detection	627:705	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	4	33	theme	magnet	1002:1007	arg1	afterward					1009:1017	a magnet afterward	1000:1017	a magnet afterward	1000:1017	The as-prepared material can quickly extract angoroside C from plasma with high extraction efficiency, be easily separated with a magnet afterward, and can be reused for at least five times.					
35880540	4	34	theme	high	947:950	arg1	efficiency					963:972	high extraction efficiency	947:972	high extraction efficiency	947:972	The as-prepared material can quickly extract angoroside C from plasma with high extraction efficiency, be easily separated with a magnet afterward, and can be reused for at least five times.					
35880540	3	35	contain	have	761:764	arg2	responsiveness					780:793	high magnetic responsiveness	766:793	high magnetic responsiveness (35.67 emu·g-1 )	766:810	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	3	35	contain	have	761:764	arg2	emu·g-1					802:808	35.67 emu·g-1	796:808	35.67 emu·g-1	796:808	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	3	35	contain	have	761:764	arg2	area					827:830	large surface area	813:830	large surface area (110.9 m2 ·g-1 )	813:847	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	3	35	contain	have	761:764	arg2	stability					861:869	strong stability	854:869	strong stability	854:869	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	3	35	contain	have	761:764	arg2	·g-1					842:845	110.9 m2 ·g-1	833:845	110.9 m2 ·g-1	833:845	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	3	35	contain	have	761:764	arg1	frameworks					750:759	The synthesized magnetic covalent-organic frameworks	708:759	The synthesized magnetic covalent-organic frameworks	708:759	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	0	36	theme	Magnetic	0:7	arg1	extraction					43:52	Magnetic covalent-organic frameworks-based extraction	0:52	Magnetic covalent-organic frameworks-based extraction	0:52	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	2	37	theme	ultra-high-performance	627:648	arg1	spectrometry					684:695	ultra-high-performance liquid chromatography-tandem mass spectrometry	627:695	ultra-high-performance liquid chromatography-tandem mass spectrometry detection	627:705	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	2	38	theme	liquid	650:655	arg1	spectrometry					684:695	ultra-high-performance liquid chromatography-tandem mass spectrometry	627:695	ultra-high-performance liquid chromatography-tandem mass spectrometry detection	627:705	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	5	39	theme	good	1123:1126	arg1	0.1-5 ng·ml-1					1139:1151	0.1-5 ng·ml-1	1139:1151	0.1-5 ng·ml-1	1139:1151	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	39	theme	good	1123:1126	arg1	linearity					1128:1136	good linearity	1123:1136	good linearity (0.1-5 ng·ml-1 )	1123:1153	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	2	40	theme	covalent-organic	455:470	arg1	frameworks					472:481	the magnetic covalent-organic frameworks	442:481	the magnetic covalent-organic frameworks	442:481	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	2	41	from	plasma	611:616	arg1	extraction					571:580	the specific extraction	558:580	the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection	558:705	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	0	42	theme	rat	147:149	arg1	plasma					151:156	rat plasma	147:156	rat plasma	147:156	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	5	43	theme	established	1067:1077	arg1	method					1079:1084	The established method	1063:1084	The established method	1063:1084	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	0	44	theme	frameworks-based	26:41	arg1	extraction					43:52	Magnetic covalent-organic frameworks-based extraction	0:52	Magnetic covalent-organic frameworks-based extraction	0:52	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	3	45	theme	large	813:817	arg1	area					827:830	large surface area	813:830	large surface area (110.9 m2 ·g-1 )	813:847	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	3	45	theme	large	813:817	arg1	·g-1					842:845	110.9 m2 ·g-1	833:845	110.9 m2 ·g-1	833:845	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	6	46	theme	pharmacokinetic	1340:1354	arg1	study					1356:1360	the pharmacokinetic study	1336:1360	the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule	1336:1446	Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.					
35880540	1	47	theme	concentration	402:414	arg1	detection					416:424	the plasma concentration detection	391:424	the plasma concentration detection	391:424	The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection.					
35880540	4	48	theme	angoroside	917:926	arg1	C					928:928	angoroside C	917:928	angoroside C	917:928	The as-prepared material can quickly extract angoroside C from plasma with high extraction efficiency, be easily separated with a magnet afterward, and can be reused for at least five times.					
35880540	2	49	theme	simple	505:510	arg1	reaction					533:540	a simple one-step Schiff base reaction	503:540	a simple one-step Schiff base reaction	503:540	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	3	50	theme	surface	819:825	arg1	area					827:830	large surface area	813:830	large surface area (110.9 m2 ·g-1 )	813:847	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	3	50	theme	surface	819:825	arg1	·g-1					842:845	110.9 m2 ·g-1	833:845	110.9 m2 ·g-1	833:845	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	3	51	theme	synthesized	712:722	arg1	frameworks					750:759	The synthesized magnetic covalent-organic frameworks	708:759	The synthesized magnetic covalent-organic frameworks	708:759	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	6	52	theme	Xuanbo	1421:1426	arg1	Granule					1440:1446	Xuanbo Shuangsheng Granule	1421:1446	Xuanbo Shuangsheng Granule	1421:1446	Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.					
35880540	1	53	theme	active	326:331	arg1	ingredient					333:342	each active ingredient	321:342	each active ingredient	321:342	The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection.					
35880540	2	54	theme	specific	562:569	arg1	extraction					571:580	the specific extraction	558:580	the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection	558:705	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	5	55	theme	percentage	1251:1260	arg1	deviation					1280:1288	percentage relative standard deviation 3.60-10.90%	1251:1300	percentage relative standard deviation 3.60-10.90%	1251:1300	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	55	theme	percentage	1251:1260	arg1	precision					1240:1248	good precision	1235:1248	good precision (percentage relative standard deviation 3.60-10.90%)	1235:1301	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	1	56	theme	traditional	238:248	arg1	medicine					258:265	the traditional Chinese medicine	234:265	the traditional Chinese medicine compound preparation	234:286	The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection.					
35880540	1	57	theme	ingredient	333:342	arg1	low					357:359	low	357:359	low	357:359	The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection.					
35880540	1	57	theme	ingredient	333:342	arg1	content					310:316	the content	306:316	the content of each active ingredient	306:342	The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection.					
35880540	2	58	theme	rat	607:609	arg1	plasma					611:616	rat plasma	607:616	rat plasma	607:616	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	0	59	from	determination	82:94	arg1	plasma					151:156	rat plasma	147:156	rat plasma	147:156	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	2	60	theme	C	602:602	arg1	extraction					571:580	the specific extraction	558:580	the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection	558:705	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	5	61	theme	quantification	1169:1182	arg1	deviation					1280:1288	percentage relative standard deviation 3.60-10.90%	1251:1300	percentage relative standard deviation 3.60-10.90%	1251:1300	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	61	theme	quantification	1169:1182	arg1	accuracy					1205:1212	good accuracy	1200:1212	good accuracy (93.18-105.36%)	1200:1228	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	61	theme	quantification	1169:1182	arg1	0.1-5 ng·ml-1					1139:1151	0.1-5 ng·ml-1	1139:1151	0.1-5 ng·ml-1	1139:1151	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	61	theme	quantification	1169:1182	arg1	precision					1240:1248	good precision	1235:1248	good precision (percentage relative standard deviation 3.60-10.90%)	1235:1301	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	61	theme	quantification	1169:1182	arg1	%					1227:1227	93.18-105.36%	1215:1227	93.18-105.36%	1215:1227	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	61	theme	quantification	1169:1182	arg1	0.1 ng·ml-1					1185:1195	0.1 ng·ml-1	1185:1195	0.1 ng·ml-1	1185:1195	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	61	theme	quantification	1169:1182	arg1	linearity					1128:1136	good linearity	1123:1136	good linearity (0.1-5 ng·ml-1 )	1123:1153	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	61	theme	quantification	1169:1182	arg1	limit					1160:1164	low limit	1156:1164	low limit of quantification (0.1 ng·ml-1 )	1156:1197	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	4	62	theme	extraction	952:961	arg1	efficiency					963:972	high extraction efficiency	947:972	high extraction efficiency	947:972	The as-prepared material can quickly extract angoroside C from plasma with high extraction efficiency, be easily separated with a magnet afterward, and can be reused for at least five times.					
35880540	0	63	theme	oral	164:167	arg1	administration					169:182	oral administration	164:182	oral administration of Xuanbo Shuangsheng Granule	164:212	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	0	64	from	C	142:142	arg1	plasma					151:156	rat plasma	147:156	rat plasma	147:156	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	4	65	theme	as-prepared	876:886	arg1	material					888:895	The as-prepared material	872:895	The as-prepared material	872:895	The as-prepared material can quickly extract angoroside C from plasma with high extraction efficiency, be easily separated with a magnet afterward, and can be reused for at least five times.					
35880540	3	66	theme	m2	839:840	arg1	area					827:830	large surface area	813:830	large surface area (110.9 m2 ·g-1 )	813:847	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	3	66	theme	m2	839:840	arg1	·g-1					842:845	110.9 m2 ·g-1	833:845	110.9 m2 ·g-1	833:845	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	0	67	theme	Xuanbo	187:192	arg1	Granule					206:212	Xuanbo Shuangsheng Granule	187:212	Xuanbo Shuangsheng Granule	187:212	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	6	68	from	C	1382:1382	arg1	rats					1387:1390	rats	1387:1390	rats after oral administration of Xuanbo Shuangsheng Granule	1387:1446	Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.					
35880540	4	69	with	plasma	935:940	arg1	efficiency					963:972	high extraction efficiency	947:972	high extraction efficiency	947:972	The as-prepared material can quickly extract angoroside C from plasma with high extraction efficiency, be easily separated with a magnet afterward, and can be reused for at least five times.					
35880540	1	70	theme	Chinese	250:256	arg1	medicine					258:265	the traditional Chinese medicine	234:265	the traditional Chinese medicine compound preparation	234:286	The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection.					
35880540	2	71	theme	angoroside	591:600	arg1	C					602:602	trace angoroside C	585:602	trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection	585:705	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	3	72	theme	magnetic	724:731	arg1	frameworks					750:759	The synthesized magnetic covalent-organic frameworks	708:759	The synthesized magnetic covalent-organic frameworks	708:759	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	0	73	from	plasma	151:156	arg1	determination					82:94	determination	82:94	determination	82:94	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	0	73	from	plasma	151:156	arg1	study					116:120	pharmacokinetic study	100:120	pharmacokinetic study	100:120	Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.					
35880540	6	74	theme	oral	1398:1401	arg1	administration					1403:1416	oral administration	1398:1416	oral administration of Xuanbo Shuangsheng Granule	1398:1446	Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.					
35880540	3	75	theme	covalent-organic	733:748	arg1	frameworks					750:759	The synthesized magnetic covalent-organic frameworks	708:759	The synthesized magnetic covalent-organic frameworks	708:759	The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability.					
35880540	1	76	theme	compound	267:274	arg1	preparation					276:286	the traditional Chinese medicine compound preparation	234:286	the traditional Chinese medicine compound preparation	234:286	The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection.					
35880540	2	77	theme	base	528:531	arg1	reaction					533:540	a simple one-step Schiff base reaction	503:540	a simple one-step Schiff base reaction	503:540	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	6	78	from	rats	1387:1390	arg1	study					1356:1360	the pharmacokinetic study	1336:1360	the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule	1336:1446	Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.					
35880540	2	79	theme	magnetic	446:453	arg1	frameworks					472:481	the magnetic covalent-organic frameworks	442:481	the magnetic covalent-organic frameworks	442:481	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	2	80	from	extraction	571:580	arg1	plasma					611:616	rat plasma	607:616	rat plasma	607:616	In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection.					
35880540	5	81	theme	standard	1271:1278	arg1	deviation					1280:1288	percentage relative standard deviation 3.60-10.90%	1251:1300	percentage relative standard deviation 3.60-10.90%	1251:1300	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	5	81	theme	standard	1271:1278	arg1	precision					1240:1248	good precision	1235:1248	good precision (percentage relative standard deviation 3.60-10.90%)	1235:1301	The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%).					
35880540	1	82	theme	preparation	276:286	arg1	complex					291:297	complex	291:297	complex	291:297	The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection.					
35880540	1	82	theme	preparation	276:286	arg1	composition					219:229	The composition	215:229	The composition of the traditional Chinese medicine compound preparation	215:286	The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection.					
35491004	8	0	theme	LPS-exposed	1153:1163	arg1	females					1165:1171	LPS-exposed females	1153:1171	LPS-exposed females	1153:1171	LPS-exposed females displayed a different behavioural phenotype with significantly increased social interaction (F(1,22) = 6.094, p = 0.018), and exploration (F(1,24) = 6.359, p = 0.015), compared to saline controls.					
35491004	9	1	theme	microbiota	1386:1395	arg1	data					1407:1410	microbiota profiling data	1386:1410	microbiota profiling data	1386:1410	With respect to microbiota profiling data, Bacteroidota was significantly increased for LPS-exposed females (F(1,14) = 4.931p = 0.035) and Proteobacteria was decreased for LPS-exposed rats of both sexes versus controls (F(1,30) = 4.923p = 0.035).					
35491004	12	2	theme	social	2100:2105	arg1	behaviour					2107:2115	social behaviour	2100:2115	social behaviour	2100:2115	These findings highlight the importance of sex in determining the impact of neonatal immune activation on social behaviour and the gut microbiota.					
35491004	3	3	from	effect	486:491	arg1	behaviour					548:556	adult behaviour	542:556	adult behaviour	542:556	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	3	3	from	effect	486:491	arg1	relationship					566:577	the relationship	562:577	the relationship to microbiome composition	562:603	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	3	4	theme	adult	542:546	arg1	behaviour					548:556	adult behaviour	542:556	adult behaviour	542:556	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	8	5	theme	different	1185:1193	arg1	phenotype					1207:1215	a different behavioural phenotype	1183:1215	a different behavioural phenotype	1183:1215	LPS-exposed females displayed a different behavioural phenotype with significantly increased social interaction (F(1,22) = 6.094, p = 0.018), and exploration (F(1,24) = 6.359, p = 0.015), compared to saline controls.					
35491004	6	6	theme	behavioural	875:885	arg1	phenotypes					887:896	distinctly different behavioural phenotypes	854:896	distinctly different behavioural phenotypes for LPS-exposed males and females	854:930	There were distinctly different behavioural phenotypes for LPS-exposed males and females.					
35491004	7	7	theme	saline	1107:1112	arg1	controls					1114:1121	saline controls	1107:1121	saline controls (F(1,23) = 8.623, p = 0.005)	1107:1150	LPS-exposed males displayed typical anxiety-like behaviours with significantly decreased social interaction (F(1,22) = 7.576, p = 0.009) and increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005).					
35491004	2	8	theme	neonatal	319:326	arg1	period					328:333	the neonatal period	315:333	the neonatal period	315:333	The microbiome gut-brain-axis, a bidirectional communication system, is plastic throughout the neonatal period and is a possible mediator of this relationship.					
35491004	12	9	theme	neonatal	2070:2077	arg1	activation					2086:2095	neonatal immune activation	2070:2095	neonatal immune activation	2070:2095	These findings highlight the importance of sex in determining the impact of neonatal immune activation on social behaviour and the gut microbiota.					
35491004	7	10	theme	relative	1095:1102	arg1	defecation					1084:1093	increased defecation	1074:1093	increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005)	1074:1150	LPS-exposed males displayed typical anxiety-like behaviours with significantly decreased social interaction (F(1,22) = 7.576, p = 0.009) and increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005).					
35491004	5	11	theme	stool	828:832	arg1	samples					834:840	stool samples	828:840	stool samples	828:840	In adulthood, behavioural tests were performed to assess anxiety-like behaviour, and microbiota sequencing was performed on stool samples.					
35491004	12	12	theme	activation	2086:2095	arg1	impact					2060:2065	the impact	2056:2065	the impact of neonatal immune activation on social behaviour and the gut microbiota	2056:2138	These findings highlight the importance of sex in determining the impact of neonatal immune activation on social behaviour and the gut microbiota.					
35491004	7	13	theme	increased	1074:1082	arg1	defecation					1084:1093	increased defecation	1074:1093	increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005)	1074:1150	LPS-exposed males displayed typical anxiety-like behaviours with significantly decreased social interaction (F(1,22) = 7.576, p = 0.009) and increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005).					
35491004	11	14	theme	life	1932:1935	arg1	behaviour					1937:1945	later life behaviour	1926:1945	later life behaviour	1926:1945	This suggests that neonatal immune activation alters both later life behaviour and adult gut microbiota in sex-specific ways.					
35491004	12	15	from	impact	2060:2065	arg1	microbiota					2129:2138	the gut microbiota	2121:2138	the gut microbiota	2121:2138	These findings highlight the importance of sex in determining the impact of neonatal immune activation on social behaviour and the gut microbiota.					
35491004	12	15	from	impact	2060:2065	arg1	behaviour					2107:2115	social behaviour	2100:2115	social behaviour	2100:2115	These findings highlight the importance of sex in determining the impact of neonatal immune activation on social behaviour and the gut microbiota.					
35491004	8	16	theme	social	1246:1251	arg1	interaction					1253:1263	significantly increased social interaction	1222:1263	significantly increased social interaction (F(1,22) = 6.094, p = 0.018)	1222:1292	LPS-exposed females displayed a different behavioural phenotype with significantly increased social interaction (F(1,22) = 6.094, p = 0.018), and exploration (F(1,24) = 6.359, p = 0.015), compared to saline controls.					
35491004	2	17	theme	communication	271:283	arg1	system					285:290	a bidirectional communication system	255:290	a bidirectional communication system	255:290	The microbiome gut-brain-axis, a bidirectional communication system, is plastic throughout the neonatal period and is a possible mediator of this relationship.					
35491004	2	17	theme	communication	271:283	arg1	gut-brain-axis					239:252	The microbiome gut-brain-axis	224:252	The microbiome gut-brain-axis	224:252	The microbiome gut-brain-axis, a bidirectional communication system, is plastic throughout the neonatal period and is a possible mediator of this relationship.					
35491004	11	18	theme	gut	1957:1959	arg1	microbiota					1961:1970	adult gut microbiota	1951:1970	adult gut microbiota	1951:1970	This suggests that neonatal immune activation alters both later life behaviour and adult gut microbiota in sex-specific ways.					
35491004	4	19	theme	postnatal	680:688	arg1	days					690:693	postnatal days 3 and 5	680:701	postnatal days 3 and 5	680:701	Wistar rats were injected with LPS (0.05 mg/kg) or saline (equivolume) on postnatal days 3 and 5.					
35491004	10	20	theme	D-glutamate	1782:1792	arg1	abundance					1753:1761	the relative abundance	1740:1761	the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females	1740:1826	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	7	21	theme	decreased	1012:1020	arg1	interaction					1029:1039	significantly decreased social interaction	998:1039	significantly decreased social interaction (F(1,22) = 7.576, p = 0.009)	998:1068	LPS-exposed males displayed typical anxiety-like behaviours with significantly decreased social interaction (F(1,22) = 7.576, p = 0.009) and increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005).					
35491004	1	22	theme	gastrointestinal	149:164	arg1	disorders					166:174	gastrointestinal disorders	149:174	gastrointestinal disorders	149:174	There is comorbidity between anxiety disorders and gastrointestinal disorders, with both linked to adverse early life events.					
35491004	12	23	theme	sex	2037:2039	arg1	importance					2023:2032	the importance	2019:2032	the importance of sex in determining the impact of neonatal immune activation on social behaviour and the gut microbiota	2019:2138	These findings highlight the importance of sex in determining the impact of neonatal immune activation on social behaviour and the gut microbiota.					
35491004	2	24	theme	bidirectional	257:269	arg1	system					285:290	a bidirectional communication system	255:290	a bidirectional communication system	255:290	The microbiome gut-brain-axis, a bidirectional communication system, is plastic throughout the neonatal period and is a possible mediator of this relationship.					
35491004	2	24	theme	bidirectional	257:269	arg1	gut-brain-axis					239:252	The microbiome gut-brain-axis	224:252	The microbiome gut-brain-axis	224:252	The microbiome gut-brain-axis, a bidirectional communication system, is plastic throughout the neonatal period and is a possible mediator of this relationship.					
35491004	10	25	theme	D-glutamine	1766:1776	arg1	abundance					1753:1761	the relative abundance	1740:1761	the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females	1740:1826	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	7	26	theme	anxiety-like	969:980	arg1	behaviours					982:991	typical anxiety-like behaviours	961:991	typical anxiety-like behaviours	961:991	LPS-exposed males displayed typical anxiety-like behaviours with significantly decreased social interaction (F(1,22) = 7.576, p = 0.009) and increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005).					
35491004	4	27	dep	days	690:693	arg1	5					701:701	5	701:701	5	701:701	Wistar rats were injected with LPS (0.05 mg/kg) or saline (equivolume) on postnatal days 3 and 5.					
35491004	4	27	dep	days	690:693	arg1	3					695:695	3	695:695	3	695:695	Wistar rats were injected with LPS (0.05 mg/kg) or saline (equivolume) on postnatal days 3 and 5.					
35491004	3	28	theme	activation	440:449	arg1	model					451:455	a well-established neonatal rodent immune activation model	398:455	a well-established neonatal rodent immune activation model	398:455	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	3	29	theme	rodent	426:431	arg1	model					451:455	a well-established neonatal rodent immune activation model	398:455	a well-established neonatal rodent immune activation model	398:455	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	0	30	theme	Neonatal	0:7	arg1	challenge					16:24	Neonatal immune challenge	0:24	Neonatal immune challenge	0:24	Neonatal immune challenge influences the microbiota and behaviour in a sexually dimorphic manner.					
35491004	7	31	theme	LPS-exposed	933:943	arg1	males					945:949	LPS-exposed males	933:949	LPS-exposed males	933:949	LPS-exposed males displayed typical anxiety-like behaviours with significantly decreased social interaction (F(1,22) = 7.576, p = 0.009) and increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005).					
35491004	11	32	theme	immune	1896:1901	arg1	activation					1903:1912	neonatal immune activation	1887:1912	neonatal immune activation	1887:1912	This suggests that neonatal immune activation alters both later life behaviour and adult gut microbiota in sex-specific ways.					
35491004	6	33	theme	different	865:873	arg1	phenotypes					887:896	distinctly different behavioural phenotypes	854:896	distinctly different behavioural phenotypes for LPS-exposed males and females	854:930	There were distinctly different behavioural phenotypes for LPS-exposed males and females.					
35491004	8	34	dep	interaction	1253:1263	arg1	F					1266:1266	F(1,22) = 6.094	1266:1280	F(1,22) = 6.094	1266:1280	LPS-exposed females displayed a different behavioural phenotype with significantly increased social interaction (F(1,22) = 6.094, p = 0.018), and exploration (F(1,24) = 6.359, p = 0.015), compared to saline controls.					
35491004	8	34	dep	interaction	1253:1263	arg1	p = 0.018					1283:1291	p = 0.018	1283:1291	p = 0.018	1283:1291	LPS-exposed females displayed a different behavioural phenotype with significantly increased social interaction (F(1,22) = 6.094, p = 0.018), and exploration (F(1,24) = 6.359, p = 0.015), compared to saline controls.					
35491004	3	35	theme	well-established	400:415	arg1	model					451:455	a well-established neonatal rodent immune activation model	398:455	a well-established neonatal rodent immune activation model	398:455	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	8	36	theme	saline	1353:1358	arg1	controls					1360:1367	saline controls	1353:1367	saline controls	1353:1367	LPS-exposed females displayed a different behavioural phenotype with significantly increased social interaction (F(1,22) = 6.094, p = 0.018), and exploration (F(1,24) = 6.359, p = 0.015), compared to saline controls.					
35491004	10	37	theme	exposed	1812:1818	arg1	females					1820:1826	LPS exposed females	1808:1826	LPS exposed females	1808:1826	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	3	38	theme	neonatal	496:503	arg1	exposure					530:537	neonatal lipopolysaccharide (LPS) exposure	496:537	neonatal lipopolysaccharide (LPS) exposure	496:537	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	3	39	used	used	393:396	arg2	we					390:391	we	390:391	we	390:391	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	8	40	dep	exploration	1299:1309	arg1	F					1312:1312	F(1,24) = 6.359	1312:1326	F(1,24) = 6.359	1312:1326	LPS-exposed females displayed a different behavioural phenotype with significantly increased social interaction (F(1,22) = 6.094, p = 0.018), and exploration (F(1,24) = 6.359, p = 0.015), compared to saline controls.					
35491004	8	40	dep	exploration	1299:1309	arg1	p = 0.015					1329:1337	p = 0.015	1329:1337	p = 0.015	1329:1337	LPS-exposed females displayed a different behavioural phenotype with significantly increased social interaction (F(1,22) = 6.094, p = 0.018), and exploration (F(1,24) = 6.359, p = 0.015), compared to saline controls.					
35491004	8	41	dep	different	1185:1193	arg1	behavioural					1195:1205	behavioural	1195:1205	behavioural	1195:1205	LPS-exposed females displayed a different behavioural phenotype with significantly increased social interaction (F(1,22) = 6.094, p = 0.018), and exploration (F(1,24) = 6.359, p = 0.015), compared to saline controls.					
35491004	12	42	theme	gut	2125:2127	arg1	microbiota					2129:2138	the gut microbiota	2121:2138	the gut microbiota	2121:2138	These findings highlight the importance of sex in determining the impact of neonatal immune activation on social behaviour and the gut microbiota.					
35491004	10	43	theme	predicted	1645:1653	arg1	pathways					1666:1673	predicted functional pathways	1645:1673	predicted functional pathways for neurotransmitters in faeces	1645:1705	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	10	44	theme	control	1840:1846	arg1	females					1848:1854	control females	1840:1854	control females (p < 0.05)	1840:1865	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	10	44	theme	control	1840:1846	arg1	p < 0.05					1857:1864	p < 0.05	1857:1864	p < 0.05	1857:1864	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	2	45	theme	relationship	370:381	arg1	plastic					296:302	plastic	296:302	plastic throughout the neonatal period	296:333	The microbiome gut-brain-axis, a bidirectional communication system, is plastic throughout the neonatal period and is a possible mediator of this relationship.					
35491004	2	45	theme	relationship	370:381	arg1	gut-brain-axis					239:252	The microbiome gut-brain-axis	224:252	The microbiome gut-brain-axis	224:252	The microbiome gut-brain-axis, a bidirectional communication system, is plastic throughout the neonatal period and is a possible mediator of this relationship.					
35491004	2	45	theme	relationship	370:381	arg1	mediator					353:360	a possible mediator	342:360	a possible mediator of this relationship	342:381	The microbiome gut-brain-axis, a bidirectional communication system, is plastic throughout the neonatal period and is a possible mediator of this relationship.					
35491004	10	46	from	alterations	1630:1640	arg1	pathways					1666:1673	predicted functional pathways	1645:1673	predicted functional pathways for neurotransmitters in faeces	1645:1705	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	11	47	from	microbiota	1961:1970	arg1	ways					1988:1991	sex-specific ways	1975:1991	sex-specific ways	1975:1991	This suggests that neonatal immune activation alters both later life behaviour and adult gut microbiota in sex-specific ways.					
35491004	1	48	attach	linked	187:192	arg2	both					182:185	both	182:185	both	182:185	There is comorbidity between anxiety disorders and gastrointestinal disorders, with both linked to adverse early life events.					
35491004	1	48	attach	linked	187:192	arg1	events					216:221	adverse early life events	197:221	adverse early life events	197:221	There is comorbidity between anxiety disorders and gastrointestinal disorders, with both linked to adverse early life events.					
35491004	10	49	dep	exposed	1812:1818	arg1	LPS					1808:1810	LPS	1808:1810	LPS	1808:1810	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	11	50	theme	sex-specific	1975:1986	arg1	ways					1988:1991	sex-specific ways	1975:1991	sex-specific ways	1975:1991	This suggests that neonatal immune activation alters both later life behaviour and adult gut microbiota in sex-specific ways.					
35491004	6	51	theme	LPS-exposed	902:912	arg1	males					914:918	LPS-exposed males	902:918	LPS-exposed males	902:918	There were distinctly different behavioural phenotypes for LPS-exposed males and females.					
35491004	11	52	from	behaviour	1937:1945	arg1	ways					1988:1991	sex-specific ways	1975:1991	sex-specific ways	1975:1991	This suggests that neonatal immune activation alters both later life behaviour and adult gut microbiota in sex-specific ways.					
35491004	4	53	theme	Wistar	606:611	arg1	rats					613:616	Wistar rats	606:616	Wistar rats	606:616	Wistar rats were injected with LPS (0.05 mg/kg) or saline (equivolume) on postnatal days 3 and 5.					
35491004	2	54	theme	possible	344:351	arg1	mediator					353:360	a possible mediator	342:360	a possible mediator of this relationship	342:381	The microbiome gut-brain-axis, a bidirectional communication system, is plastic throughout the neonatal period and is a possible mediator of this relationship.					
35491004	9	55	theme	profiling	1397:1405	arg1	data					1407:1410	microbiota profiling data	1386:1410	microbiota profiling data	1386:1410	With respect to microbiota profiling data, Bacteroidota was significantly increased for LPS-exposed females (F(1,14) = 4.931p = 0.035) and Proteobacteria was decreased for LPS-exposed rats of both sexes versus controls (F(1,30) = 4.923p = 0.035).					
35491004	10	56	from	neurotransmitters	1679:1695	arg1	faeces					1700:1705	faeces	1700:1705	faeces	1700:1705	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	10	57	theme	relative	1744:1751	arg1	abundance					1753:1761	the relative abundance	1740:1761	the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females	1740:1826	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	1	58	theme	adverse	197:203	arg1	events					216:221	adverse early life events	197:221	adverse early life events	197:221	There is comorbidity between anxiety disorders and gastrointestinal disorders, with both linked to adverse early life events.					
35491004	3	59	theme	microbiome	582:591	arg1	composition					593:603	microbiome composition	582:603	microbiome composition	582:603	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	3	60	theme	lipopolysaccharide	505:522	arg1	exposure					530:537	neonatal lipopolysaccharide (LPS) exposure	496:537	neonatal lipopolysaccharide (LPS) exposure	496:537	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	11	61	theme	later	1926:1930	arg1	behaviour					1937:1945	later life behaviour	1926:1945	later life behaviour	1926:1945	This suggests that neonatal immune activation alters both later life behaviour and adult gut microbiota in sex-specific ways.					
35491004	1	62	theme	life	211:214	arg1	events					216:221	adverse early life events	197:221	adverse early life events	197:221	There is comorbidity between anxiety disorders and gastrointestinal disorders, with both linked to adverse early life events.					
35491004	10	63	dep	D-glutamine	1766:1776	arg1	metabolism					1794:1803	metabolism	1794:1803	metabolism	1794:1803	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	7	64	dep	controls	1114:1121	arg1	p = 0.005					1141:1149	p = 0.005	1141:1149	p = 0.005	1141:1149	LPS-exposed males displayed typical anxiety-like behaviours with significantly decreased social interaction (F(1,22) = 7.576, p = 0.009) and increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005).					
35491004	7	64	dep	controls	1114:1121	arg1	F					1124:1124	F(1,23) = 8.623	1124:1138	F(1,23) = 8.623	1124:1138	LPS-exposed males displayed typical anxiety-like behaviours with significantly decreased social interaction (F(1,22) = 7.576, p = 0.009) and increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005).					
35491004	7	65	theme	p = 0.009	1059:1067	arg1	F					1042:1042	F(1,22) = 7.576	1042:1056	F(1,22) = 7.576	1042:1056	LPS-exposed males displayed typical anxiety-like behaviours with significantly decreased social interaction (F(1,22) = 7.576, p = 0.009) and increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005).					
35491004	9	66	theme	LPS-exposed	1458:1468	arg1	F					1479:1479	F(1,14) = 4.931p = 0.035	1479:1502	F(1,14) = 4.931p = 0.035	1479:1502	With respect to microbiota profiling data, Bacteroidota was significantly increased for LPS-exposed females (F(1,14) = 4.931p = 0.035) and Proteobacteria was decreased for LPS-exposed rats of both sexes versus controls (F(1,30) = 4.923p = 0.035).					
35491004	9	66	theme	LPS-exposed	1458:1468	arg1	females					1470:1476	LPS-exposed females	1458:1476	LPS-exposed females (F(1,14) = 4.931p = 0.035)	1458:1503	With respect to microbiota profiling data, Bacteroidota was significantly increased for LPS-exposed females (F(1,14) = 4.931p = 0.035) and Proteobacteria was decreased for LPS-exposed rats of both sexes versus controls (F(1,30) = 4.923p = 0.035).					
35491004	8	67	theme	increased	1236:1244	arg1	interaction					1253:1263	significantly increased social interaction	1222:1263	significantly increased social interaction (F(1,22) = 6.094, p = 0.018)	1222:1292	LPS-exposed females displayed a different behavioural phenotype with significantly increased social interaction (F(1,22) = 6.094, p = 0.018), and exploration (F(1,24) = 6.359, p = 0.015), compared to saline controls.					
35491004	11	68	theme	adult	1951:1955	arg1	microbiota					1961:1970	adult gut microbiota	1951:1970	adult gut microbiota	1951:1970	This suggests that neonatal immune activation alters both later life behaviour and adult gut microbiota in sex-specific ways.					
35491004	1	69	theme	anxiety	127:133	arg1	disorders					135:143	anxiety disorders	127:143	anxiety disorders	127:143	There is comorbidity between anxiety disorders and gastrointestinal disorders, with both linked to adverse early life events.					
35491004	0	70	theme	dimorphic	80:88	arg1	manner					90:95	a sexually dimorphic manner	69:95	a sexually dimorphic manner	69:95	Neonatal immune challenge influences the microbiota and behaviour in a sexually dimorphic manner.					
35491004	7	71	theme	social	1022:1027	arg1	interaction					1029:1039	significantly decreased social interaction	998:1039	significantly decreased social interaction (F(1,22) = 7.576, p = 0.009)	998:1068	LPS-exposed males displayed typical anxiety-like behaviours with significantly decreased social interaction (F(1,22) = 7.576, p = 0.009) and increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005).					
35491004	10	72	from	decrease	1728:1735	arg1	abundance					1753:1761	the relative abundance	1740:1761	the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females	1740:1826	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	5	73	theme	behavioural	718:728	arg1	tests					730:734	behavioural tests	718:734	behavioural tests	718:734	In adulthood, behavioural tests were performed to assess anxiety-like behaviour, and microbiota sequencing was performed on stool samples.					
35491004	1	74	theme	early	205:209	arg1	events					216:221	adverse early life events	197:221	adverse early life events	197:221	There is comorbidity between anxiety disorders and gastrointestinal disorders, with both linked to adverse early life events.					
35491004	10	75	from	abundance	1753:1761	arg1	females					1820:1826	LPS exposed females	1808:1826	LPS exposed females	1808:1826	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	5	76	theme	microbiota	789:798	arg1	sequencing					800:809	microbiota sequencing	789:809	microbiota sequencing	789:809	In adulthood, behavioural tests were performed to assess anxiety-like behaviour, and microbiota sequencing was performed on stool samples.					
35491004	7	77	theme	typical	961:967	arg1	behaviours					982:991	typical anxiety-like behaviours	961:991	typical anxiety-like behaviours	961:991	LPS-exposed males displayed typical anxiety-like behaviours with significantly decreased social interaction (F(1,22) = 7.576, p = 0.009) and increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005).					
35491004	3	78	theme	immune	433:438	arg1	model					451:455	a well-established neonatal rodent immune activation model	398:455	a well-established neonatal rodent immune activation model	398:455	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	9	79	theme	LPS-exposed	1542:1552	arg1	rats					1554:1557	LPS-exposed rats	1542:1557	LPS-exposed rats of both sexes	1542:1571	With respect to microbiota profiling data, Bacteroidota was significantly increased for LPS-exposed females (F(1,14) = 4.931p = 0.035) and Proteobacteria was decreased for LPS-exposed rats of both sexes versus controls (F(1,30) = 4.923p = 0.035).					
35491004	7	80	dep	interaction	1029:1039	arg1	F					1042:1042	F(1,22) = 7.576	1042:1056	F(1,22) = 7.576	1042:1056	LPS-exposed males displayed typical anxiety-like behaviours with significantly decreased social interaction (F(1,22) = 7.576, p = 0.009) and increased defecation relative to saline controls (F(1,23) = 8.623, p = 0.005).					
35491004	3	81	theme	long-term	476:484	arg1	effect					486:491	the long-term effect	472:491	the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition	472:603	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	5	82	theme	anxiety-like	761:772	arg1	behaviour					774:782	anxiety-like behaviour	761:782	anxiety-like behaviour	761:782	In adulthood, behavioural tests were performed to assess anxiety-like behaviour, and microbiota sequencing was performed on stool samples.					
35491004	3	83	theme	neonatal	417:424	arg1	model					451:455	a well-established neonatal rodent immune activation model	398:455	a well-established neonatal rodent immune activation model	398:455	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	11	84	theme	neonatal	1887:1894	arg1	activation					1903:1912	neonatal immune activation	1887:1912	neonatal immune activation	1887:1912	This suggests that neonatal immune activation alters both later life behaviour and adult gut microbiota in sex-specific ways.					
35491004	9	85	theme	sexes	1567:1571	arg1	rats					1554:1557	LPS-exposed rats	1542:1557	LPS-exposed rats of both sexes	1542:1571	With respect to microbiota profiling data, Bacteroidota was significantly increased for LPS-exposed females (F(1,14) = 4.931p = 0.035) and Proteobacteria was decreased for LPS-exposed rats of both sexes versus controls (F(1,30) = 4.923p = 0.035).					
35491004	9	86	dep	data	1407:1410	arg1	respect					1375:1381	respect	1375:1381	respect	1375:1381	With respect to microbiota profiling data, Bacteroidota was significantly increased for LPS-exposed females (F(1,14) = 4.931p = 0.035) and Proteobacteria was decreased for LPS-exposed rats of both sexes versus controls (F(1,30) = 4.923p = 0.035).					
35491004	10	87	theme	functional	1655:1664	arg1	pathways					1666:1673	predicted functional pathways	1645:1673	predicted functional pathways for neurotransmitters in faeces	1645:1705	Furthermore, alterations in predicted functional pathways for neurotransmitters in faeces were observed with a decrease in the relative abundance of D-glutamine and D-glutamate metabolism in LPS exposed females compared to control females (p < 0.05).					
35491004	2	88	theme	microbiome	228:237	arg1	system					285:290	a bidirectional communication system	255:290	a bidirectional communication system	255:290	The microbiome gut-brain-axis, a bidirectional communication system, is plastic throughout the neonatal period and is a possible mediator of this relationship.					
35491004	2	88	theme	microbiome	228:237	arg1	gut-brain-axis					239:252	The microbiome gut-brain-axis	224:252	The microbiome gut-brain-axis	224:252	The microbiome gut-brain-axis, a bidirectional communication system, is plastic throughout the neonatal period and is a possible mediator of this relationship.					
35491004	2	88	theme	microbiome	228:237	arg1	plastic					296:302	plastic	296:302	plastic throughout the neonatal period	296:333	The microbiome gut-brain-axis, a bidirectional communication system, is plastic throughout the neonatal period and is a possible mediator of this relationship.					
35491004	3	89	theme	exposure	530:537	arg1	effect					486:491	the long-term effect	472:491	the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition	472:603	Here, we used a well-established neonatal rodent immune activation model to investigate the long-term effect of neonatal lipopolysaccharide (LPS) exposure on adult behaviour and the relationship to microbiome composition.					
35491004	12	90	theme	immune	2079:2084	arg1	activation					2086:2095	neonatal immune activation	2070:2095	neonatal immune activation	2070:2095	These findings highlight the importance of sex in determining the impact of neonatal immune activation on social behaviour and the gut microbiota.					
35491004	0	91	theme	immune	9:14	arg1	challenge					16:24	Neonatal immune challenge	0:24	Neonatal immune challenge	0:24	Neonatal immune challenge influences the microbiota and behaviour in a sexually dimorphic manner.					
37298671	0	0	theme	Biostimulants	95:107	arg1	Effects					71:77	the Positive Effects	58:77	the Positive Effects of Protein-Rich Biostimulants on Sugar Beet Plant Development	58:139	Metabolic Processes and Biological Macromolecules Defined the Positive Effects of Protein-Rich Biostimulants on Sugar Beet Plant Development.					
37298671	3	1	theme	metabolic	567:575	arg1	processes					577:585	metabolic processes	567:585	metabolic processes	567:585	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	5	2	from	growth	906:911	arg1	soil					920:923	HNC soil	916:923	HNC soil	916:923	Sucrose and total sugar content in the roots were high in NS-treated plants and correlated to root growth in HNC soil.					
37298671	9	3	theme	g/kg	1737:1740	arg1	soil					1742:1745	2 g/kg soil	1735:1745	2 g/kg soil	1735:1745	Thus, the PBBs enhanced protein-related traits in the plants through a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil).					
37298671	9	3	theme	g/kg	1737:1740	arg1	amounts					1726:1732	certain amounts	1718:1732	certain amounts (2 g/kg soil)	1718:1746	Thus, the PBBs enhanced protein-related traits in the plants through a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil).					
37298671	2	4	theme	protein	344:350	arg1	PBBs					473:476	PBBs	473:476	PBBs	473:476	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	4	theme	protein	344:350	arg1	PF					358:359	PF	358:359	PF	358:359	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	4	theme	protein	344:350	arg1	film					352:355	potato protein film	337:355	potato protein film (PF)	337:360	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	4	theme	protein	344:350	arg1	gluten					320:325	hydrolyzed wheat gluten	303:325	hydrolyzed wheat gluten (HWG)	303:331	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	5	5	from	Sucrose	807:813	arg1	roots					846:850	the roots	842:850	the roots	842:850	Sucrose and total sugar content in the roots were high in NS-treated plants and correlated to root growth in HNC soil.					
37298671	3	6	theme	nutrient	678:685	arg1	NS					697:698	NS	697:698	NS	697:698	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	3	6	theme	nutrient	678:685	arg1	solution					687:694	nutrient solution	678:694	nutrient solution (NS)	678:699	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	0	7	theme	Protein-Rich	82:93	arg1	Biostimulants					95:107	Protein-Rich Biostimulants	82:107	Protein-Rich Biostimulants	82:107	Metabolic Processes and Biological Macromolecules Defined the Positive Effects of Protein-Rich Biostimulants on Sugar Beet Plant Development.					
37298671	3	8	with	comparison	620:629	arg1	control					650:656	control	650:656	control	650:656	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	3	8	with	comparison	620:629	arg1	treatment					663:671	treatment	663:671	treatment with nutrient solution (NS)	663:699	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	3	8	with	comparison	620:629	arg1	treatment					639:647	no treatment	636:647	no treatment (control)	636:657	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	2	9	theme	potato	337:342	arg1	PBBs					473:476	PBBs	473:476	PBBs	473:476	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	9	theme	potato	337:342	arg1	PF					358:359	PF	358:359	PF	358:359	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	9	theme	potato	337:342	arg1	film					352:355	potato protein film	337:355	potato protein film (PF)	337:360	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	9	theme	potato	337:342	arg1	gluten					320:325	hydrolyzed wheat gluten	303:325	hydrolyzed wheat gluten (HWG)	303:331	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	10	from	gluten	320:325	arg1	soil					390:393	1 and 2 g/kg soil	377:393	1 and 2 g/kg soil	377:393	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	10	from	gluten	320:325	arg1	soils					417:421	two different soils	403:421	two different soils (low and high nutrient; LNC and HNC)	403:458	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	10	from	gluten	320:325	arg1	levels					369:374	two levels	365:374	two levels (1 and 2 g/kg soil)	365:394	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	9	11	theme	related	1611:1617	arg1	genes					1605:1609	genes	1605:1609	genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil)	1605:1746	Thus, the PBBs enhanced protein-related traits in the plants through a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil).					
37298671	0	12	theme	Sugar	112:116	arg1	Development					129:139	Sugar Beet Plant Development	112:139	Sugar Beet Plant Development	112:139	Metabolic Processes and Biological Macromolecules Defined the Positive Effects of Protein-Rich Biostimulants on Sugar Beet Plant Development.					
37298671	7	13	theme	transcriptomic	1164:1177	arg1	analysis					1179:1186	The transcriptomic analysis	1160:1186	The transcriptomic analysis	1160:1186	The transcriptomic analysis revealed that genes associated with ribosomes and photosynthesis were upregulated in the leaf samples of plants treated with either HWG or PP compared to the control.					
37298671	10	14	theme	beet	1801:1804	arg1	roots					1786:1790	the roots	1782:1790	the roots of sugar beet	1782:1804	However, sucrose accumulation in the roots of sugar beet seemed to be related to the easy availability of nitrogen.					
37298671	10	15	theme	sucrose	1758:1764	arg1	accumulation					1766:1777	sucrose accumulation	1758:1777	sucrose accumulation in the roots of sugar beet	1758:1804	However, sucrose accumulation in the roots of sugar beet seemed to be related to the easy availability of nitrogen.					
37298671	6	16	dep	HWG	1064:1066	arg1	mostly					1053:1058	mostly	1053:1058	mostly	1053:1058	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	1	17	contain	have	177:180	arg1	PBBs					171:174	PBBs	171:174	PBBs	171:174	Protein-based biostimulants (PBBs) have a positive effect on plant development, although the biological background for this effect is not well understood.					
37298671	1	17	contain	have	177:180	arg1	biostimulants					156:168	Protein-based biostimulants	142:168	Protein-based biostimulants (PBBs)	142:175	Protein-based biostimulants (PBBs) have a positive effect on plant development, although the biological background for this effect is not well understood.					
37298671	1	17	contain	have	177:180	arg2	effect					193:198	a positive effect	182:198	a positive effect	182:198	Protein-based biostimulants (PBBs) have a positive effect on plant development, although the biological background for this effect is not well understood.					
37298671	8	18	theme	root	1463:1466	arg1	samples					1468:1474	root samples	1463:1474	root samples of HWG or PF-treated plants	1463:1502	Furthermore, genes associated with the biosynthesis of secondary metabolites were largely down-regulated in root samples of HWG or PF-treated plants.					
37298671	9	19	theme	increased	1669:1677	arg1	growth					1685:1690	increased plant growth	1669:1690	increased plant growth	1669:1690	Thus, the PBBs enhanced protein-related traits in the plants through a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil).					
37298671	0	20	theme	Plant	123:127	arg1	Development					129:139	Sugar Beet Plant Development	112:139	Sugar Beet Plant Development	112:139	Metabolic Processes and Biological Macromolecules Defined the Positive Effects of Protein-Rich Biostimulants on Sugar Beet Plant Development.					
37298671	10	21	theme	easy	1834:1837	arg1	availability					1839:1850	the easy availability	1830:1850	the easy availability of nitrogen	1830:1862	However, sucrose accumulation in the roots of sugar beet seemed to be related to the easy availability of nitrogen.					
37298671	3	22	with	treatment	639:647	arg1	NS					697:698	NS	697:698	NS	697:698	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	3	22	with	treatment	639:647	arg1	solution					687:694	nutrient solution	678:694	nutrient solution (NS)	678:699	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	5	23	from	content	831:837	arg1	roots					846:850	the roots	842:850	the roots	842:850	Sucrose and total sugar content in the roots were high in NS-treated plants and correlated to root growth in HNC soil.					
37298671	5	24	theme	root	901:904	arg1	growth					906:911	root growth	901:911	root growth in HNC soil	901:923	Sucrose and total sugar content in the roots were high in NS-treated plants and correlated to root growth in HNC soil.					
37298671	0	25	theme	Beet	118:121	arg1	Development					129:139	Sugar Beet Plant Development	112:139	Sugar Beet Plant Development	112:139	Metabolic Processes and Biological Macromolecules Defined the Positive Effects of Protein-Rich Biostimulants on Sugar Beet Plant Development.					
37298671	5	26	theme	HNC	916:918	arg1	soil					920:923	HNC soil	916:923	HNC soil	916:923	Sucrose and total sugar content in the roots were high in NS-treated plants and correlated to root growth in HNC soil.					
37298671	1	27	theme	positive	184:191	arg1	effect					193:198	a positive effect	182:198	a positive effect	182:198	Protein-based biostimulants (PBBs) have a positive effect on plant development, although the biological background for this effect is not well understood.					
37298671	8	28	theme	metabolites	1420:1430	arg1	biosynthesis					1394:1405	the biosynthesis	1390:1405	the biosynthesis of secondary metabolites	1390:1430	Furthermore, genes associated with the biosynthesis of secondary metabolites were largely down-regulated in root samples of HWG or PF-treated plants.					
37298671	6	29	from	%	1097:1097	arg1	LNC					1120:1122	LNC	1120:1122	LNC	1120:1122	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	6	29	from	%	1097:1097	arg1	HNC					1112:1114	HNC	1112:1114	HNC	1112:1114	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	4	30	theme	significant	723:733	arg1	enhancement					742:752	a significant growth enhancement	721:752	a significant growth enhancement of the plants using HWG and PF across the two soils	721:804	The results showed a significant growth enhancement of the plants using HWG and PF across the two soils.					
37298671	0	31	theme	Metabolic	0:8	arg1	Processes					10:18	Metabolic Processes	0:18	Metabolic Processes	0:18	Metabolic Processes and Biological Macromolecules Defined the Positive Effects of Protein-Rich Biostimulants on Sugar Beet Plant Development.					
37298671	6	32	theme	RuBisCO	998:1004	arg1	contents					1006:1013	RuBisCO contents	998:1013	RuBisCO contents	998:1013	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	10	33	theme	nitrogen	1855:1862	arg1	availability					1839:1850	the easy availability	1830:1850	the easy availability of nitrogen	1830:1862	However, sucrose accumulation in the roots of sugar beet seemed to be related to the easy availability of nitrogen.					
37298671	2	34	theme	wheat	314:318	arg1	film					352:355	potato protein film	337:355	potato protein film (PF)	337:360	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	34	theme	wheat	314:318	arg1	PBBs					473:476	PBBs	473:476	PBBs	473:476	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	34	theme	wheat	314:318	arg1	HWG					328:330	HWG	328:330	HWG	328:330	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	34	theme	wheat	314:318	arg1	gluten					320:325	hydrolyzed wheat gluten	303:325	hydrolyzed wheat gluten (HWG)	303:331	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	9	35	theme	plant	1679:1683	arg1	growth					1685:1690	increased plant growth	1669:1690	increased plant growth	1669:1690	Thus, the PBBs enhanced protein-related traits in the plants through a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil).					
37298671	9	36	from	traits	1545:1550	arg1	plants					1559:1564	the plants	1555:1564	the plants	1555:1564	Thus, the PBBs enhanced protein-related traits in the plants through a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil).					
37298671	0	37	theme	Biological	24:33	arg1	Macromolecules					35:48	Biological Macromolecules	24:48	Biological Macromolecules	24:48	Metabolic Processes and Biological Macromolecules Defined the Positive Effects of Protein-Rich Biostimulants on Sugar Beet Plant Development.					
37298671	5	38	theme	sugar	825:829	arg1	content					831:837	total sugar content	819:837	total sugar content	819:837	Sucrose and total sugar content in the roots were high in NS-treated plants and correlated to root growth in HNC soil.					
37298671	2	39	dep	nutrient	437:444	arg1	HNC					455:457	HNC	455:457	HNC	455:457	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	39	dep	nutrient	437:444	arg1	LNC					447:449	LNC	447:449	LNC	447:449	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	40	from	film	352:355	arg1	soil					390:393	1 and 2 g/kg soil	377:393	1 and 2 g/kg soil	377:393	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	40	from	film	352:355	arg1	soils					417:421	two different soils	403:421	two different soils (low and high nutrient; LNC and HNC)	403:458	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	40	from	film	352:355	arg1	levels					369:374	two levels	365:374	two levels (1 and 2 g/kg soil)	365:394	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	41	theme	hydrolyzed	303:312	arg1	film					352:355	potato protein film	337:355	potato protein film (PF)	337:360	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	41	theme	hydrolyzed	303:312	arg1	PBBs					473:476	PBBs	473:476	PBBs	473:476	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	41	theme	hydrolyzed	303:312	arg1	HWG					328:330	HWG	328:330	HWG	328:330	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	41	theme	hydrolyzed	303:312	arg1	gluten					320:325	hydrolyzed wheat gluten	303:325	hydrolyzed wheat gluten (HWG)	303:331	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	3	42	from	effect	483:488	arg1	protein					533:539	protein	533:539	protein	533:539	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	3	42	from	effect	483:488	arg1	peptides					546:553	peptides	546:553	peptides	546:553	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	3	42	from	effect	483:488	arg1	sugars					525:530	sugars	525:530	sugars	525:530	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	3	42	from	effect	483:488	arg1	processes					577:585	metabolic processes	567:585	metabolic processes	567:585	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	3	42	from	effect	483:488	arg1	traits					517:522	agronomic traits	507:522	agronomic traits	507:522	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	1	43	theme	plant	203:207	arg1	development					209:219	plant development	203:219	plant development	203:219	Protein-based biostimulants (PBBs) have a positive effect on plant development, although the biological background for this effect is not well understood.					
37298671	6	44	theme	protein	944:950	arg1	composition					952:962	protein composition	944:962	protein composition	944:962	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	8	45	theme	plants	1497:1502	arg1	samples					1468:1474	root samples	1463:1474	root samples of HWG or PF-treated plants	1463:1502	Furthermore, genes associated with the biosynthesis of secondary metabolites were largely down-regulated in root samples of HWG or PF-treated plants.					
37298671	10	46	from	accumulation	1766:1777	arg1	roots					1786:1790	the roots	1782:1790	the roots of sugar beet	1782:1804	However, sucrose accumulation in the roots of sugar beet seemed to be related to the easy availability of nitrogen.					
37298671	2	47	theme	different	407:415	arg1	soils					417:421	two different soils	403:421	two different soils (low and high nutrient; LNC and HNC)	403:458	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	3	48	theme	sugar	606:610	arg1	beet					612:615	sugar beet	606:615	sugar beet	606:615	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	7	49	theme	plants	1293:1298	arg1	samples					1282:1288	the leaf samples	1273:1288	the leaf samples of plants treated with either HWG or PP compared to the control	1273:1352	The transcriptomic analysis revealed that genes associated with ribosomes and photosynthesis were upregulated in the leaf samples of plants treated with either HWG or PP compared to the control.					
37298671	6	50	theme	PBB-treated	1033:1043	arg1	plants					1045:1050	PBB-treated plants	1033:1050	PBB-treated plants (mostly for HWG and PF at 2 g/kg soil)	1033:1089	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	8	51	theme	PF-treated	1486:1495	arg1	plants					1497:1502	HWG or PF-treated plants	1479:1502	HWG or PF-treated plants	1479:1502	Furthermore, genes associated with the biosynthesis of secondary metabolites were largely down-regulated in root samples of HWG or PF-treated plants.					
37298671	9	52	theme	higher	1576:1581	arg1	rate					1597:1600	a higher transcription rate	1574:1600	a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil)	1574:1746	Thus, the PBBs enhanced protein-related traits in the plants through a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil).					
37298671	3	53	theme	no	636:637	arg1	control					650:656	control	650:656	control	650:656	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	3	53	theme	no	636:637	arg1	treatment					639:647	no treatment	636:647	no treatment (control)	636:657	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	7	54	theme	leaf	1277:1280	arg1	samples					1282:1288	the leaf samples	1273:1288	the leaf samples of plants treated with either HWG or PP compared to the control	1273:1352	The transcriptomic analysis revealed that genes associated with ribosomes and photosynthesis were upregulated in the leaf samples of plants treated with either HWG or PP compared to the control.					
37298671	2	55	theme	g/kg	385:388	arg1	soil					390:393	1 and 2 g/kg soil	377:393	1 and 2 g/kg soil	377:393	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	55	theme	g/kg	385:388	arg1	levels					369:374	two levels	365:374	two levels (1 and 2 g/kg soil)	365:394	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	3	56	with	treatment	663:671	arg1	NS					697:698	NS	697:698	NS	697:698	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	3	56	with	treatment	663:671	arg1	solution					687:694	nutrient solution	678:694	nutrient solution (NS)	678:699	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	2	57	theme	high	432:435	arg1	nutrient					437:444	low and high nutrient	424:444	low and high nutrient; LNC and HNC	424:457	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	0	58	theme	Positive	62:69	arg1	Effects					71:77	the Positive Effects	58:77	the Positive Effects of Protein-Rich Biostimulants on Sugar Beet Plant Development	58:139	Metabolic Processes and Biological Macromolecules Defined the Positive Effects of Protein-Rich Biostimulants on Sugar Beet Plant Development.					
37298671	5	59	from	plants	876:881	arg1	high					857:860	high	857:860	high	857:860	Sucrose and total sugar content in the roots were high in NS-treated plants and correlated to root growth in HNC soil.					
37298671	4	60	theme	growth	735:740	arg1	enhancement					742:752	a significant growth enhancement	721:752	a significant growth enhancement of the plants using HWG and PF across the two soils	721:804	The results showed a significant growth enhancement of the plants using HWG and PF across the two soils.					
37298671	0	61	from	Effects	71:77	arg1	Development					129:139	Sugar Beet Plant Development	112:139	Sugar Beet Plant Development	112:139	Metabolic Processes and Biological Macromolecules Defined the Positive Effects of Protein-Rich Biostimulants on Sugar Beet Plant Development.					
37298671	2	62	dep	soils	417:421	arg1	nutrient					437:444	low and high nutrient	424:444	low and high nutrient; LNC and HNC	424:457	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	6	63	theme	related	933:939	arg1	contents					1006:1013	RuBisCO contents	998:1013	RuBisCO contents	998:1013	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	6	63	theme	related	933:939	arg1	Traits					926:931	Traits	926:931	Traits	926:931	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	6	63	theme	related	933:939	arg1	peptide					985:991	peptide	985:991	peptide	985:991	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	6	63	theme	related	933:939	arg1	nitrogen					975:982	nitrogen	975:982	nitrogen	975:982	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	10	64	theme	sugar	1795:1799	arg1	beet					1801:1804	sugar beet	1795:1804	sugar beet	1795:1804	However, sucrose accumulation in the roots of sugar beet seemed to be related to the easy availability of nitrogen.					
37298671	6	65	from	soil	1085:1088	arg1	PF					1072:1073	PF	1072:1073	PF	1072:1073	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	6	65	from	soil	1085:1088	arg1	HWG					1064:1066	HWG	1064:1066	HWG	1064:1066	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	6	66	theme	g/kg	1080:1083	arg1	soil					1085:1088	2 g/kg soil	1078:1088	2 g/kg soil	1078:1088	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	3	67	theme	agronomic	507:515	arg1	traits					517:522	agronomic traits	507:522	agronomic traits	507:522	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	9	68	theme	protein-related	1529:1543	arg1	traits					1545:1550	protein-related traits	1529:1550	protein-related traits in the plants	1529:1564	Thus, the PBBs enhanced protein-related traits in the plants through a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil).					
37298671	1	69	theme	Protein-based	142:154	arg1	PBBs					171:174	PBBs	171:174	PBBs	171:174	Protein-based biostimulants (PBBs) have a positive effect on plant development, although the biological background for this effect is not well understood.					
37298671	1	69	theme	Protein-based	142:154	arg1	biostimulants					156:168	Protein-based biostimulants	142:168	Protein-based biostimulants (PBBs)	142:175	Protein-based biostimulants (PBBs) have a positive effect on plant development, although the biological background for this effect is not well understood.					
37298671	9	70	theme	genes	1605:1609	arg1	rate					1597:1600	a higher transcription rate	1574:1600	a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil)	1574:1746	Thus, the PBBs enhanced protein-related traits in the plants through a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil).					
37298671	2	71	used	used	465:468	arg2	gluten					320:325	hydrolyzed wheat gluten	303:325	hydrolyzed wheat gluten (HWG)	303:331	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	71	used	used	465:468	arg2	PF					358:359	PF	358:359	PF	358:359	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	71	used	used	465:468	arg2	film					352:355	potato protein film	337:355	potato protein film (PF)	337:360	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	71	used	used	465:468	arg2	HWG					328:330	HWG	328:330	HWG	328:330	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	2	71	used	used	465:468	arg2	PBBs					473:476	PBBs	473:476	PBBs	473:476	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	9	72	theme	certain	1718:1724	arg1	soil					1742:1745	2 g/kg soil	1735:1745	2 g/kg soil	1735:1745	Thus, the PBBs enhanced protein-related traits in the plants through a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil).					
37298671	9	72	theme	certain	1718:1724	arg1	amounts					1726:1732	certain amounts	1718:1732	certain amounts (2 g/kg soil)	1718:1746	Thus, the PBBs enhanced protein-related traits in the plants through a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil).					
37298671	5	73	theme	NS-treated	865:874	arg1	plants					876:881	NS-treated plants	865:881	NS-treated plants	865:881	Sucrose and total sugar content in the roots were high in NS-treated plants and correlated to root growth in HNC soil.					
37298671	5	74	theme	total	819:823	arg1	content					831:837	total sugar content	819:837	total sugar content	819:837	Sucrose and total sugar content in the roots were high in NS-treated plants and correlated to root growth in HNC soil.					
37298671	6	75	from	%	1107:1107	arg1	LNC					1120:1122	LNC	1120:1122	LNC	1120:1122	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	6	75	from	%	1107:1107	arg1	HNC					1112:1114	HNC	1112:1114	HNC	1112:1114	Traits related to protein composition, including nitrogen, peptide, and RuBisCO contents, were enhanced in PBB-treated plants (mostly for HWG and PF at 2 g/kg soil) by 100% and >250% in HNC and LNC, respectively, compared to control.					
37298671	8	76	theme	HWG	1479:1481	arg1	plants					1497:1502	HWG or PF-treated plants	1479:1502	HWG or PF-treated plants	1479:1502	Furthermore, genes associated with the biosynthesis of secondary metabolites were largely down-regulated in root samples of HWG or PF-treated plants.					
37298671	8	77	theme	secondary	1410:1418	arg1	metabolites					1420:1430	secondary metabolites	1410:1430	secondary metabolites	1410:1430	Furthermore, genes associated with the biosynthesis of secondary metabolites were largely down-regulated in root samples of HWG or PF-treated plants.					
37298671	1	78	theme	biological	235:244	arg1	background					246:255	the biological background	231:255	the biological background for this effect	231:271	Protein-based biostimulants (PBBs) have a positive effect on plant development, although the biological background for this effect is not well understood.					
37298671	4	79	theme	plants	761:766	arg1	enhancement					742:752	a significant growth enhancement	721:752	a significant growth enhancement of the plants using HWG and PF across the two soils	721:804	The results showed a significant growth enhancement of the plants using HWG and PF across the two soils.					
37298671	3	80	theme	PBBs	499:502	arg1	effect					483:488	The effect	479:488	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes,	479:586	The effect of these PBBs on agronomic traits, sugars, protein, and peptides, as well as metabolic processes, were evaluated on sugar beet in comparison with no treatment (control) and treatment with nutrient solution (NS).					
37298671	2	81	theme	low	424:426	arg1	nutrient					437:444	low and high nutrient	424:444	low and high nutrient; LNC and HNC	424:457	Here, hydrolyzed wheat gluten (HWG) and potato protein film (PF) in two levels (1 and 2 g/kg soil) and in two different soils (low and high nutrient; LNC and HNC) were used as PBBs.					
37298671	5	82	from	high	857:860	arg1	plants					876:881	NS-treated plants	865:881	NS-treated plants	865:881	Sucrose and total sugar content in the roots were high in NS-treated plants and correlated to root growth in HNC soil.					
37298671	9	83	theme	transcription	1583:1595	arg1	rate					1597:1600	a higher transcription rate	1574:1600	a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil)	1574:1746	Thus, the PBBs enhanced protein-related traits in the plants through a higher transcription rate of genes related to protein- and photosynthesis, which resulted in increased plant growth, especially when added in certain amounts (2 g/kg soil).					
36569196	9	0	theme	stabilizers	1724:1734	arg1	effect					1689:1694	the effect	1685:1694	the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin)	1685:1776	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	4	1	theme	phages	540:545	arg1	selection					527:535	the selection	523:535	the selection of phages for therapeutic use	523:565	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	11	2	from	storage	2142:2148	arg1	volume					2158:2163	1 ml volume	2153:2163	1 ml volume	2153:2163	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	5	3	dep	hydrogels	752:760	arg1	Purilon					780:786	Purilon	780:786	Purilon	780:786	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	5	3	dep	hydrogels	752:760	arg1	Intrasite					793:801	Intrasite	793:801	Intrasite	793:801	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	5	3	dep	hydrogels	752:760	arg1	Askina					772:777	Askina	772:777	Askina	772:777	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	5	3	dep	hydrogels	752:760	arg1	hydrogels					752:760	four commercially available hydrogels	724:760	four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus	724:938	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	7	4	theme	different	1324:1332	arg1	morphologies					1334:1345	different morphologies	1324:1345	different morphologies	1324:1345	We then optimized the assay for both optical density and microscopy readers using GrowDex as matrix with 23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes.					
36569196	4	5	theme	therapeutic	551:561	arg1	use					563:565	therapeutic use	551:565	therapeutic use	551:565	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	11	6	from	positives	2107:2115	arg1	PST					2120:2122	PST	2120:2122	PST	2120:2122	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	8	7	theme	density	1569:1575	arg1	reader					1577:1582	optical density reader	1561:1582	optical density reader	1561:1582	When the bacterial growth was monitored by microscopy reader, the PST was executed in just 3 hours, and there was no need for overnight culturing bacterial cells prior to the assay, whereas using optical density reader, bacteria had to be pre-cultured overnight, and the assay time was five hours.					
36569196	9	8	theme	hyaluronic	1748:1757	arg1	acid					1759:1762	hyaluronic acid	1748:1762	hyaluronic acid	1748:1762	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	9	8	theme	hyaluronic	1748:1757	arg1	stabilizers					1724:1734	three different chemical stabilizers	1699:1734	three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin)	1699:1776	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	8	9	theme	overnight	1491:1499	arg1	cells					1521:1525	overnight culturing bacterial cells	1491:1525	overnight culturing bacterial cells prior to the assay	1491:1544	When the bacterial growth was monitored by microscopy reader, the PST was executed in just 3 hours, and there was no need for overnight culturing bacterial cells prior to the assay, whereas using optical density reader, bacteria had to be pre-cultured overnight, and the assay time was five hours.					
36569196	7	10	theme	genome	1351:1356	arg1	sizes					1358:1362	genome sizes	1351:1362	genome sizes	1351:1362	We then optimized the assay for both optical density and microscopy readers using GrowDex as matrix with 23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes.					
36569196	7	11	theme	different	1281:1289	arg1	species					1291:1297	10 different species	1278:1297	10 different species	1278:1297	We then optimized the assay for both optical density and microscopy readers using GrowDex as matrix with 23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes.					
36569196	11	12	theme	tested	2068:2073	arg1	positives					2107:2115	positives	2107:2115	positives in PST after a six-month storage in 1 ml volume	2107:2163	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	11	12	theme	tested	2068:2073	arg1	phages					2075:2080	all tested phages	2064:2080	all tested phages	2064:2080	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	2	13	theme	bacterial	276:284	arg1	pathogens					286:294	bacterial pathogens	276:294	bacterial pathogens	276:294	Due to the narrow host range of phages, hundreds to thousands of phages are required to cover the diversity of bacterial pathogens.					
36569196	10	14	dep	behaved	1851:1857	arg1	phages					1838:1843	These phages	1832:1843	These phages	1832:1843	These phages assay behaved very differently in respect to the chemical stabilizers, and there was not a single stabilizer suitable for all phages.					
36569196	4	15	theme	"	602:602	arg1	concept					610:616	a "ready-to-screen" plate concept	584:616	a "ready-to-screen" plate concept	584:616	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	12	16	from	plates	2187:2192	arg1	profound					2247:2254	profound	2247:2254	profound	2247:2254	In "ready-to-screen" plates, the differences in phage stabilities were even more profound, varying from two to six months for the most and least stable phages, respectively.					
36569196	12	16	from	plates	2187:2192	arg1	differences					2199:2209	the differences	2195:2209	the differences in phage stabilities	2195:2230	In "ready-to-screen" plates, the differences in phage stabilities were even more profound, varying from two to six months for the most and least stable phages, respectively.					
36569196	4	17	theme	study	435:439	arg1	aims					422:425	The aims	418:425	The aims of this study	418:439	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	7	18	theme	bacterial	1247:1255	arg1	strains					1257:1263	23 bacterial strains	1244:1263	23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes	1244:1362	We then optimized the assay for both optical density and microscopy readers using GrowDex as matrix with 23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes.					
36569196	9	19	theme	six-month	1783:1791	arg1	assay					1803:1807	a six-month stability assay	1781:1807	a six-month stability assay with six model phages	1781:1829	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	9	20	dep	stabilizers	1724:1734	arg1	acid					1759:1762	hyaluronic acid	1748:1762	hyaluronic acid	1748:1762	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	9	20	dep	stabilizers	1724:1734	arg1	gelatin					1769:1775	gelatin	1769:1775	gelatin	1769:1775	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	9	20	dep	stabilizers	1724:1734	arg1	stabilizers					1724:1734	three different chemical stabilizers	1699:1734	three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin)	1699:1776	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	9	20	dep	stabilizers	1724:1734	arg1	trehalose					1737:1745	trehalose	1737:1745	trehalose	1737:1745	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	4	21	theme	small	666:670	arg1	droplets					672:679	small droplets	666:679	small droplets in microtiter plate wells	666:705	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	4	21	theme	small	666:670	arg1	phages					625:630	phages	625:630	phages	625:630	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	3	22	from	therapy	319:325	arg1	essential					384:392	essential	384:392	essential	384:392	In personalized phage therapy, fast selection of the phages for individual patients is essential for successful therapy.					
36569196	8	23	theme	assay	1636:1640	arg1	time					1642:1645	the assay time	1632:1645	the assay time	1632:1645	When the bacterial growth was monitored by microscopy reader, the PST was executed in just 3 hours, and there was no need for overnight culturing bacterial cells prior to the assay, whereas using optical density reader, bacteria had to be pre-cultured overnight, and the assay time was five hours.					
36569196	8	23	theme	assay	1636:1640	arg1	hours					1656:1660	five hours	1651:1660	five hours	1651:1660	When the bacterial growth was monitored by microscopy reader, the PST was executed in just 3 hours, and there was no need for overnight culturing bacterial cells prior to the assay, whereas using optical density reader, bacteria had to be pre-cultured overnight, and the assay time was five hours.					
36569196	1	24	theme	antibiotic	134:143	arg1	bacteria					155:162	antibiotic resistant bacteria	134:162	antibiotic resistant bacteria	134:162	Phage therapy is one alternative to cure infections caused by antibiotic resistant bacteria.					
36569196	8	25	theme	bacterial	1374:1382	arg1	growth					1384:1389	the bacterial growth	1370:1389	the bacterial growth	1370:1389	When the bacterial growth was monitored by microscopy reader, the PST was executed in just 3 hours, and there was no need for overnight culturing bacterial cells prior to the assay, whereas using optical density reader, bacteria had to be pre-cultured overnight, and the assay time was five hours.					
36569196	6	26	theme	bacterial	1083:1091	arg1	culture					1093:1099	bacterial culture	1083:1099	bacterial culture	1083:1099	Of these four hydrogels, GrowDex was the best matrix for PST, as it did not inhibit bacterial growth, released phages quickly when mixed with bacterial culture, and maintained phage viability well.					
36569196	7	27	with	matrix	1232:1237	arg1	strains					1257:1263	23 bacterial strains	1244:1263	23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes	1244:1362	We then optimized the assay for both optical density and microscopy readers using GrowDex as matrix with 23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes.					
36569196	2	28	theme	phages	230:235	arg1	thousands					217:225	thousands	217:225	thousands of phages	217:235	Due to the narrow host range of phages, hundreds to thousands of phages are required to cover the diversity of bacterial pathogens.					
36569196	8	29	theme	bacterial	1511:1519	arg1	cells					1521:1525	overnight culturing bacterial cells	1491:1525	overnight culturing bacterial cells prior to the assay	1491:1544	When the bacterial growth was monitored by microscopy reader, the PST was executed in just 3 hours, and there was no need for overnight culturing bacterial cells prior to the assay, whereas using optical density reader, bacteria had to be pre-cultured overnight, and the assay time was five hours.					
36569196	3	30	theme	personalized	300:311	arg1	therapy					319:325	personalized phage therapy	300:325	personalized phage therapy	300:325	In personalized phage therapy, fast selection of the phages for individual patients is essential for successful therapy.					
36569196	4	31	theme	rapid	458:462	arg1	PST					514:516	PST	514:516	PST	514:516	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	4	31	theme	rapid	458:462	arg1	assay					507:511	a rapid hydrogel-based liquid phage susceptibility assay	456:511	a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use	456:565	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	5	32	theme	phage	829:833	arg1	matrices					835:842	phage matrices	829:842	phage matrices	829:842	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	4	33	theme	liquid	479:484	arg1	PST					514:516	PST	514:516	PST	514:516	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	4	33	theme	liquid	479:484	arg1	assay					507:511	a rapid hydrogel-based liquid phage susceptibility assay	456:511	a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use	456:565	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	5	34	theme	which	877:881	arg1	which					877:881	which	877:881	which	877:881	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	5	34	theme	which	877:881	arg1	two					870:872	two	870:872	two	870:872	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	5	34	theme	which	877:881	arg1	phages					862:867	four phages	857:867	four phages	857:867	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	3	35	theme	fast	328:331	arg1	selection					333:341	fast selection	328:341	fast selection of the phages for individual patients	328:379	In personalized phage therapy, fast selection of the phages for individual patients is essential for successful therapy.					
36569196	5	36	with	PSTs	847:850	arg1	which					877:881	which	877:881	which	877:881	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	5	36	with	PSTs	847:850	arg1	two					870:872	two	870:872	two	870:872	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	5	36	with	PSTs	847:850	arg1	phages					862:867	four phages	857:867	four phages	857:867	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	6	37	theme	best	982:985	arg1	GrowDex					966:972	GrowDex	966:972	GrowDex	966:972	Of these four hydrogels, GrowDex was the best matrix for PST, as it did not inhibit bacterial growth, released phages quickly when mixed with bacterial culture, and maintained phage viability well.					
36569196	6	37	theme	best	982:985	arg1	matrix					987:992	the best matrix	978:992	the best matrix for PST	978:1000	Of these four hydrogels, GrowDex was the best matrix for PST, as it did not inhibit bacterial growth, released phages quickly when mixed with bacterial culture, and maintained phage viability well.					
36569196	3	38	theme	individual	361:370	arg1	patients					372:379	individual patients	361:379	individual patients	361:379	In personalized phage therapy, fast selection of the phages for individual patients is essential for successful therapy.					
36569196	0	39	theme	Rapid	0:4	arg1	susceptibility					27:40	Rapid hydrogel-based phage susceptibility	0:40	Rapid hydrogel-based phage susceptibility	0:40	Rapid hydrogel-based phage susceptibility test for pathogenic bacteria.					
36569196	3	40	theme	phages	350:355	arg1	selection					333:341	fast selection	328:341	fast selection of the phages for individual patients	328:379	In personalized phage therapy, fast selection of the phages for individual patients is essential for successful therapy.					
36569196	11	41	theme	1 ml	2153:2156	arg1	volume					2158:2163	1 ml volume	2153:2163	1 ml volume	2153:2163	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	4	42	theme	plate	695:699	arg1	wells					701:705	microtiter plate wells	684:705	microtiter plate wells	684:705	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	0	43	theme	phage	21:25	arg1	susceptibility					27:40	Rapid hydrogel-based phage susceptibility	0:40	Rapid hydrogel-based phage susceptibility	0:40	Rapid hydrogel-based phage susceptibility test for pathogenic bacteria.					
36569196	1	44	theme	cure	108:111	arg1	infections					113:122	cure infections	108:122	cure infections caused by antibiotic resistant bacteria	108:162	Phage therapy is one alternative to cure infections caused by antibiotic resistant bacteria.					
36569196	2	45	theme	narrow	176:181	arg1	range					188:192	the narrow host range	172:192	the narrow host range of phages	172:202	Due to the narrow host range of phages, hundreds to thousands of phages are required to cover the diversity of bacterial pathogens.					
36569196	7	46	contain	possessing	1313:1322	arg2	morphologies					1334:1345	different morphologies	1324:1345	different morphologies	1324:1345	We then optimized the assay for both optical density and microscopy readers using GrowDex as matrix with 23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes.					
36569196	7	46	contain	possessing	1313:1322	arg1	phages					1306:1311	23 phages	1303:1311	23 phages possessing different morphologies and genome sizes	1303:1362	We then optimized the assay for both optical density and microscopy readers using GrowDex as matrix with 23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes.					
36569196	7	46	contain	possessing	1313:1322	arg2	sizes					1358:1362	genome sizes	1351:1362	genome sizes	1351:1362	We then optimized the assay for both optical density and microscopy readers using GrowDex as matrix with 23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes.					
36569196	7	47	theme	optical	1176:1182	arg1	density					1184:1190	optical density	1176:1190	optical density	1176:1190	We then optimized the assay for both optical density and microscopy readers using GrowDex as matrix with 23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes.					
36569196	4	48	theme	susceptibility	492:505	arg1	PST					514:516	PST	514:516	PST	514:516	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	4	48	theme	susceptibility	492:505	arg1	assay					507:511	a rapid hydrogel-based liquid phage susceptibility assay	456:511	a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use	456:565	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	5	49	theme	available	742:750	arg1	Purilon					780:786	Purilon	780:786	Purilon	780:786	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	5	49	theme	available	742:750	arg1	Intrasite					793:801	Intrasite	793:801	Intrasite	793:801	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	5	49	theme	available	742:750	arg1	Askina					772:777	Askina	772:777	Askina	772:777	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	5	49	theme	available	742:750	arg1	hydrogels					752:760	four commercially available hydrogels	724:760	four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus	724:938	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	10	50	theme	chemical	1894:1901	arg1	stabilizers					1903:1913	the chemical stabilizers	1890:1913	the chemical stabilizers	1890:1913	These phages assay behaved very differently in respect to the chemical stabilizers, and there was not a single stabilizer suitable for all phages.					
36569196	12	51	theme	ready-to-screen	2170:2184	arg1	plates					2187:2192	"ready-to-screen" plates	2169:2192	"ready-to-screen" plates	2169:2192	In "ready-to-screen" plates, the differences in phage stabilities were even more profound, varying from two to six months for the most and least stable phages, respectively.					
36569196	2	52	theme	phages	197:202	arg1	range					188:192	the narrow host range	172:192	the narrow host range of phages	172:202	Due to the narrow host range of phages, hundreds to thousands of phages are required to cover the diversity of bacterial pathogens.					
36569196	12	53	theme	least	2305:2309	arg1	phages					2318:2323	the most and least stable phages	2292:2323	phages	2318:2323	In "ready-to-screen" plates, the differences in phage stabilities were even more profound, varying from two to six months for the most and least stable phages, respectively.					
36569196	9	54	theme	chemical	1715:1722	arg1	acid					1759:1762	hyaluronic acid	1748:1762	hyaluronic acid	1748:1762	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	9	54	theme	chemical	1715:1722	arg1	stabilizers					1724:1734	three different chemical stabilizers	1699:1734	three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin)	1699:1776	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	9	54	theme	chemical	1715:1722	arg1	trehalose					1737:1745	trehalose	1737:1745	trehalose	1737:1745	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	9	54	theme	chemical	1715:1722	arg1	gelatin					1769:1775	gelatin	1769:1775	gelatin	1769:1775	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	10	55	theme	single	1936:1941	arg1	stabilizer					1943:1952	a single stabilizer	1934:1952	a single stabilizer suitable for all phages	1934:1976	These phages assay behaved very differently in respect to the chemical stabilizers, and there was not a single stabilizer suitable for all phages.					
36569196	8	56	theme	culturing	1501:1509	arg1	cells					1521:1525	overnight culturing bacterial cells	1491:1525	overnight culturing bacterial cells prior to the assay	1491:1544	When the bacterial growth was monitored by microscopy reader, the PST was executed in just 3 hours, and there was no need for overnight culturing bacterial cells prior to the assay, whereas using optical density reader, bacteria had to be pre-cultured overnight, and the assay time was five hours.					
36569196	8	57	theme	optical	1561:1567	arg1	reader					1577:1582	optical density reader	1561:1582	optical density reader	1561:1582	When the bacterial growth was monitored by microscopy reader, the PST was executed in just 3 hours, and there was no need for overnight culturing bacterial cells prior to the assay, whereas using optical density reader, bacteria had to be pre-cultured overnight, and the assay time was five hours.					
36569196	0	58	theme	pathogenic	51:60	arg1	bacteria					62:69	pathogenic bacteria	51:69	pathogenic bacteria	51:69	Rapid hydrogel-based phage susceptibility test for pathogenic bacteria.					
36569196	4	59	theme	plate	604:608	arg1	concept					610:616	a "ready-to-screen" plate concept	584:616	a "ready-to-screen" plate concept	584:616	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	8	60	theme	microscopy	1408:1417	arg1	reader					1419:1424	microscopy reader	1408:1424	microscopy reader	1408:1424	When the bacterial growth was monitored by microscopy reader, the PST was executed in just 3 hours, and there was no need for overnight culturing bacterial cells prior to the assay, whereas using optical density reader, bacteria had to be pre-cultured overnight, and the assay time was five hours.					
36569196	12	61	theme	phage	2214:2218	arg1	stabilities					2220:2230	phage stabilities	2214:2230	phage stabilities	2214:2230	In "ready-to-screen" plates, the differences in phage stabilities were even more profound, varying from two to six months for the most and least stable phages, respectively.					
36569196	12	62	from	differences	2199:2209	arg1	stabilities					2220:2230	phage stabilities	2214:2230	phage stabilities	2214:2230	In "ready-to-screen" plates, the differences in phage stabilities were even more profound, varying from two to six months for the most and least stable phages, respectively.					
36569196	2	63	theme	pathogens	286:294	arg1	diversity					263:271	the diversity	259:271	the diversity of bacterial pathogens	259:294	Due to the narrow host range of phages, hundreds to thousands of phages are required to cover the diversity of bacterial pathogens.					
36569196	6	64	theme	bacterial	1025:1033	arg1	growth					1035:1040	bacterial growth	1025:1040	bacterial growth	1025:1040	Of these four hydrogels, GrowDex was the best matrix for PST, as it did not inhibit bacterial growth, released phages quickly when mixed with bacterial culture, and maintained phage viability well.					
36569196	10	65	theme	suitable	1954:1961	arg1	stabilizer					1943:1952	a single stabilizer	1934:1952	a single stabilizer suitable for all phages	1934:1976	These phages assay behaved very differently in respect to the chemical stabilizers, and there was not a single stabilizer suitable for all phages.					
36569196	6	66	theme	phage	1117:1121	arg1	viability					1123:1131	phage viability	1117:1131	phage viability	1117:1131	Of these four hydrogels, GrowDex was the best matrix for PST, as it did not inhibit bacterial growth, released phages quickly when mixed with bacterial culture, and maintained phage viability well.					
36569196	4	67	theme	ready-to-screen	587:601	arg1	concept					610:616	a "ready-to-screen" plate concept	584:616	a "ready-to-screen" plate concept	584:616	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	9	68	theme	stability	1793:1801	arg1	assay					1803:1807	a six-month stability assay	1781:1807	a six-month stability assay with six model phages	1781:1829	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	5	69	dep	which	877:881	arg1	infecting					883:891	infecting	883:891	infecting Escherichia coli and two Staphylococcus aureus	883:938	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	1	70	theme	resistant	145:153	arg1	bacteria					155:162	antibiotic resistant bacteria	134:162	antibiotic resistant bacteria	134:162	Phage therapy is one alternative to cure infections caused by antibiotic resistant bacteria.					
36569196	11	71	theme	hyaluronic	2012:2021	arg1	gelatin					1993:1999	gelatin	1993:1999	gelatin (0.01%)	1993:2007	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	11	71	theme	hyaluronic	2012:2021	arg1	stabilizer					2052:2061	stabilizer	2052:2061	stabilizer	2052:2061	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	11	71	theme	hyaluronic	2012:2021	arg1	acid					2023:2026	hyaluronic acid	2012:2026	hyaluronic acid (0.2 mg/ml)	2012:2038	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	11	71	theme	hyaluronic	2012:2021	arg1	mg/ml					2033:2037	0.2 mg/ml	2029:2037	0.2 mg/ml	2029:2037	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	4	72	theme	hydrogel-based	464:477	arg1	PST					514:516	PST	514:516	PST	514:516	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	4	72	theme	hydrogel-based	464:477	arg1	assay					507:511	a rapid hydrogel-based liquid phage susceptibility assay	456:511	a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use	456:565	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	9	73	theme	model	1818:1822	arg1	phages					1824:1829	six model phages	1814:1829	six model phages	1814:1829	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	3	74	from	essential	384:392	arg1	therapy					319:325	personalized phage therapy	300:325	personalized phage therapy	300:325	In personalized phage therapy, fast selection of the phages for individual patients is essential for successful therapy.					
36569196	3	75	theme	phage	313:317	arg1	therapy					319:325	personalized phage therapy	300:325	personalized phage therapy	300:325	In personalized phage therapy, fast selection of the phages for individual patients is essential for successful therapy.					
36569196	4	76	theme	phage	486:490	arg1	PST					514:516	PST	514:516	PST	514:516	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	4	76	theme	phage	486:490	arg1	assay					507:511	a rapid hydrogel-based liquid phage susceptibility assay	456:511	a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use	456:565	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	11	77	theme	six-month	2132:2140	arg1	storage					2142:2148	a six-month storage	2130:2148	a six-month storage in 1 ml volume	2130:2163	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	9	78	with	assay	1803:1807	arg1	phages					1824:1829	six model phages	1814:1829	six model phages	1814:1829	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	1	79	theme	Phage	72:76	arg1	therapy					78:84	Phage therapy	72:84	Phage therapy	72:84	Phage therapy is one alternative to cure infections caused by antibiotic resistant bacteria.					
36569196	5	80	from	suitability	814:824	arg1	PSTs					847:850	PSTs	847:850	PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus	847:938	We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus.					
36569196	12	81	from	profound	2247:2254	arg1	plates					2187:2192	"ready-to-screen" plates	2169:2192	"ready-to-screen" plates	2169:2192	In "ready-to-screen" plates, the differences in phage stabilities were even more profound, varying from two to six months for the most and least stable phages, respectively.					
36569196	0	82	theme	hydrogel-based	6:19	arg1	susceptibility					27:40	Rapid hydrogel-based phage susceptibility	0:40	Rapid hydrogel-based phage susceptibility	0:40	Rapid hydrogel-based phage susceptibility test for pathogenic bacteria.					
36569196	7	83	theme	microscopy	1196:1205	arg1	readers					1207:1213	both optical density and microscopy readers	1171:1213	both optical density and microscopy readers	1171:1213	We then optimized the assay for both optical density and microscopy readers using GrowDex as matrix with 23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes.					
36569196	12	84	theme	stable	2311:2316	arg1	phages					2318:2323	the most and least stable phages	2292:2323	phages	2318:2323	In "ready-to-screen" plates, the differences in phage stabilities were even more profound, varying from two to six months for the most and least stable phages, respectively.					
36569196	4	85	from	droplets	672:679	arg1	wells					701:705	microtiter plate wells	684:705	microtiter plate wells	684:705	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	7	86	theme	density	1184:1190	arg1	readers					1207:1213	both optical density and microscopy readers	1171:1213	both optical density and microscopy readers	1171:1213	We then optimized the assay for both optical density and microscopy readers using GrowDex as matrix with 23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes.					
36569196	2	87	theme	host	183:186	arg1	range					188:192	the narrow host range	172:192	the narrow host range of phages	172:202	Due to the narrow host range of phages, hundreds to thousands of phages are required to cover the diversity of bacterial pathogens.					
36569196	4	88	theme	microtiter	684:693	arg1	wells					701:705	microtiter plate wells	684:705	microtiter plate wells	684:705	The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a "ready-to-screen" plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells.					
36569196	6	89	theme	hydrogels	955:963	arg1	GrowDex					966:972	GrowDex	966:972	GrowDex	966:972	Of these four hydrogels, GrowDex was the best matrix for PST, as it did not inhibit bacterial growth, released phages quickly when mixed with bacterial culture, and maintained phage viability well.					
36569196	6	89	theme	hydrogels	955:963	arg1	matrix					987:992	the best matrix	978:992	the best matrix for PST	978:1000	Of these four hydrogels, GrowDex was the best matrix for PST, as it did not inhibit bacterial growth, released phages quickly when mixed with bacterial culture, and maintained phage viability well.					
36569196	3	90	theme	successful	398:407	arg1	therapy					409:415	successful therapy	398:415	successful therapy	398:415	In personalized phage therapy, fast selection of the phages for individual patients is essential for successful therapy.					
36569196	12	91	theme	"	2185:2185	arg1	plates					2187:2192	"ready-to-screen" plates	2169:2192	"ready-to-screen" plates	2169:2192	In "ready-to-screen" plates, the differences in phage stabilities were even more profound, varying from two to six months for the most and least stable phages, respectively.					
36569196	12	92	dep	six	2277:2279	arg1	to					2274:2275	to	2274:2275	to	2274:2275	In "ready-to-screen" plates, the differences in phage stabilities were even more profound, varying from two to six months for the most and least stable phages, respectively.					
36569196	11	93	used	used	2044:2047	arg2	mg/ml					2033:2037	0.2 mg/ml	2029:2037	0.2 mg/ml	2029:2037	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	11	93	used	used	2044:2047	arg2	%					2006:2006	0.01%	2002:2006	0.01%	2002:2006	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	11	93	used	used	2044:2047	arg2	gelatin					1993:1999	gelatin	1993:1999	gelatin (0.01%)	1993:2007	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	11	93	used	used	2044:2047	arg2	acid					2023:2026	hyaluronic acid	2012:2026	hyaluronic acid (0.2 mg/ml)	2012:2038	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	11	93	used	used	2044:2047	arg2	stabilizer					2052:2061	stabilizer	2052:2061	stabilizer	2052:2061	However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume.					
36569196	9	94	theme	different	1705:1713	arg1	acid					1759:1762	hyaluronic acid	1748:1762	hyaluronic acid	1748:1762	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	9	94	theme	different	1705:1713	arg1	stabilizers					1724:1734	three different chemical stabilizers	1699:1734	three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin)	1699:1776	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	9	94	theme	different	1705:1713	arg1	trehalose					1737:1745	trehalose	1737:1745	trehalose	1737:1745	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36569196	9	94	theme	different	1705:1713	arg1	gelatin					1769:1775	gelatin	1769:1775	gelatin	1769:1775	Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages.					
36948263	4	0	theme	field	856:860	arg1	sites					862:866	three experimental field sites	837:866	three experimental field sites in the Molise	837:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	10	1	theme	transcription	1900:1912	arg1	factor					1914:1919	NF-κB transcription factor	1894:1919	NF-κB transcription factor	1894:1919	These effects were correlated to a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor.					
36948263	14	2	theme	inflammatory	2384:2395	arg1	processes					2397:2405	inflammatory processes	2384:2405	inflammatory processes	2384:2405	Next, we demonstrated that EOs were able to reduce CD4+ T cell activation which are also involved in inflammatory processes.					
36948263	15	3	theme	T	2568:2568	arg1	response					2591:2598	CD4+ T cells-mediate immune response	2563:2598	CD4+ T cells-mediate immune response	2563:2598	CONCLUSIONS Our data describe for the first time that chamomile EOs exerted their anti-inflammatory and antioxidant activity by modulating macrophages and CD4+ T cells-mediate immune response.					
36948263	7	4	theme	high	1447:1450	arg1	content					1452:1458	a high content	1445:1458	a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils	1445:1546	RESULTS The chemical analysis of the samples revealed the presence of a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils.					
36948263	8	5	theme	LPS/IFN-γ-induced	1606:1622	arg1	inflammation					1624:1635	LPS/IFN-γ-induced inflammation	1606:1635	LPS/IFN-γ-induced inflammation	1606:1635	GC1, GC2 and GC3 essential oils significantly attenuated LPS/IFN-γ-induced inflammation by reducing M1 polarization.					
36948263	3	6	theme	most	537:540	arg1	diseases					572:579	most infectious and non-infectious diseases	537:579	most infectious and non-infectious diseases	537:579	Chronic inflammation is involved in the pathogenesis of most infectious and non-infectious diseases and macrophages are considered the major cellular players that drive disease initiation and maintenance.					
36948263	5	7	theme	gas	1179:1181	arg1	spectrometry					1203:1214	gas chromatography-mass spectrometry	1179:1214	gas chromatography-mass spectrometry (GC-MS)	1179:1222	MATERIAL AND METHODS Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS).					
36948263	5	7	theme	gas	1179:1181	arg1	GC-MS					1217:1221	GC-MS	1217:1221	GC-MS	1217:1221	MATERIAL AND METHODS Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS).					
36948263	4	8	theme	plants	820:825	arg1	oil					796:798	the essential oil	782:798	the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise	782:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	12	9	theme	rapid	2153:2157	arg1	scavenging					2159:2168	the rapid scavenging	2149:2168	the rapid scavenging of ROS	2149:2175	Our study demonstrated that GC1, GC2 and GC3 were highly effective at increasing GCL and HMOX-1 anti-oxidant enzymes expression leading to the rapid scavenging of ROS.					
36948263	1	10	from	use	274:276	arg1	medicine					293:300	traditional medicine	281:300	traditional medicine	281:300	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	3	11	theme	diseases	572:579	arg1	pathogenesis					521:532	the pathogenesis	517:532	the pathogenesis of most infectious and non-infectious diseases	517:579	Chronic inflammation is involved in the pathogenesis of most infectious and non-infectious diseases and macrophages are considered the major cellular players that drive disease initiation and maintenance.					
36948263	12	12	theme	anti-oxidant	2106:2117	arg1	expression					2127:2136	HMOX-1 anti-oxidant enzymes expression	2099:2136	HMOX-1 anti-oxidant enzymes expression	2099:2136	Our study demonstrated that GC1, GC2 and GC3 were highly effective at increasing GCL and HMOX-1 anti-oxidant enzymes expression leading to the rapid scavenging of ROS.					
36948263	0	13	theme	therapeutic	119:129	arg1	action					131:136	a therapeutic action	117:136	a therapeutic action	117:136	Chamomile essential oils exert anti-inflammatory effects involving human and murine macrophages: Evidence to support a therapeutic action.					
36948263	1	14	theme	herbaceous	205:214	arg1	plant					216:220	an herbaceous plant	202:220	an herbaceous plant	202:220	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	1	14	theme	herbaceous	205:214	arg1	RELEVANCE					160:168	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile	139:178	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.)	139:197	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	6	15	theme	murine	1292:1297	arg1	macrophages					1299:1309	pro-inflammatory murine macrophages	1275:1309	pro-inflammatory murine macrophages	1275:1309	The Eos were tested for their ability to modulate pro-inflammatory murine macrophages and human peripheral blood mononuclear cells (PBMCs) functions.					
36948263	5	16	theme	called	1058:1063	arg1	GC1					1065:1067	called GC1	1058:1067	called GC1	1058:1067	MATERIAL AND METHODS Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS).					
36948263	9	17	theme	anti-inflammatory	1702:1718	arg1	property					1720:1727	significant anti-inflammatory property	1690:1727	significant anti-inflammatory property	1690:1727	In details, they showed significant anti-inflammatory property by inhibiting NO, TNF-α and IL-6 production.					
36948263	3	18	theme	cellular	622:629	arg1	players					631:637	the major cellular players	612:637	the major cellular players that drive disease initiation and maintenance	612:683	Chronic inflammation is involved in the pathogenesis of most infectious and non-infectious diseases and macrophages are considered the major cellular players that drive disease initiation and maintenance.					
36948263	4	19	theme	study	719:723	arg1	aim					707:709	The aim	703:709	The aim of this study	703:723	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	14	20	theme	CD4+	2334:2337	arg1	activation					2346:2355	CD4+ T cell activation	2334:2355	CD4+ T cell activation which are also involved in inflammatory processes	2334:2405	Next, we demonstrated that EOs were able to reduce CD4+ T cell activation which are also involved in inflammatory processes.					
36948263	1	21	from	Astereaceae	234:244	arg1	plant					216:220	an herbaceous plant	202:220	an herbaceous plant	202:220	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	1	21	from	Astereaceae	234:244	arg1	RELEVANCE					160:168	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile	139:178	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.)	139:197	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	1	22	theme	ETHNOPHARMACOLOGICAL	139:158	arg1	plant					216:220	an herbaceous plant	202:220	an herbaceous plant	202:220	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	1	22	theme	ETHNOPHARMACOLOGICAL	139:158	arg1	RELEVANCE					160:168	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile	139:178	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.)	139:197	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	3	23	theme	major	616:620	arg1	players					631:637	the major cellular players	612:637	the major cellular players that drive disease initiation and maintenance	612:683	Chronic inflammation is involved in the pathogenesis of most infectious and non-infectious diseases and macrophages are considered the major cellular players that drive disease initiation and maintenance.					
36948263	15	24	theme	first	2446:2450	arg1	time					2452:2455	the first time	2442:2455	the first time	2442:2455	CONCLUSIONS Our data describe for the first time that chamomile EOs exerted their anti-inflammatory and antioxidant activity by modulating macrophages and CD4+ T cells-mediate immune response.					
36948263	7	25	dep	RESULTS	1375:1381	arg1	analysis					1396:1403	The chemical analysis	1383:1403	RESULTS The chemical analysis of the samples	1375:1418	RESULTS The chemical analysis of the samples revealed the presence of a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils.					
36948263	7	26	theme	entire	1536:1541	arg1	oils					1543:1546	the entire oils	1532:1546	the entire oils	1532:1546	RESULTS The chemical analysis of the samples revealed the presence of a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils.					
36948263	9	27	theme	IL-6	1757:1760	arg1	production					1762:1771	IL-6 production	1757:1771	IL-6 production	1757:1771	In details, they showed significant anti-inflammatory property by inhibiting NO, TNF-α and IL-6 production.					
36948263	6	28	theme	mononuclear	1338:1348	arg1	PBMCs					1357:1361	PBMCs	1357:1361	PBMCs	1357:1361	The Eos were tested for their ability to modulate pro-inflammatory murine macrophages and human peripheral blood mononuclear cells (PBMCs) functions.					
36948263	6	28	theme	mononuclear	1338:1348	arg1	cells					1350:1354	human peripheral blood mononuclear cells	1315:1354	human peripheral blood mononuclear cells (PBMCs)	1315:1362	The Eos were tested for their ability to modulate pro-inflammatory murine macrophages and human peripheral blood mononuclear cells (PBMCs) functions.					
36948263	4	29	theme	chemical	762:769	arg1	profile					771:777	the chemical profile	758:777	the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise	758:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	2	30	theme	several	371:377	arg1	diseases					379:386	several diseases	371:386	several diseases	371:386	It has been used as herbal remedies for thousands of years to treat several diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders.					
36948263	2	30	theme	several	371:377	arg1	infections					399:408	infections	399:408	infections	399:408	It has been used as herbal remedies for thousands of years to treat several diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders.					
36948263	6	31	theme	peripheral	1321:1330	arg1	PBMCs					1357:1361	PBMCs	1357:1361	PBMCs	1357:1361	The Eos were tested for their ability to modulate pro-inflammatory murine macrophages and human peripheral blood mononuclear cells (PBMCs) functions.					
36948263	6	31	theme	peripheral	1321:1330	arg1	cells					1350:1354	human peripheral blood mononuclear cells	1315:1354	human peripheral blood mononuclear cells (PBMCs)	1315:1362	The Eos were tested for their ability to modulate pro-inflammatory murine macrophages and human peripheral blood mononuclear cells (PBMCs) functions.					
36948263	2	32	used	used	315:318	arg2	It					303:304	It	303:304	It	303:304	It has been used as herbal remedies for thousands of years to treat several diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders.					
36948263	2	32	used	used	315:318	arg2	remedies					330:337	herbal remedies	323:337	herbal remedies for thousands of years	323:360	It has been used as herbal remedies for thousands of years to treat several diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders.					
36948263	0	33	theme	Chamomile	0:8	arg1	oils					20:23	Chamomile essential oils	0:23	Chamomile essential oils	0:23	Chamomile essential oils exert anti-inflammatory effects involving human and murine macrophages: Evidence to support a therapeutic action.					
36948263	5	34	theme	chamomilla	1122:1131	arg1	parts					1110:1114	aerial parts	1103:1114	aerial parts of M. chamomilla	1103:1131	MATERIAL AND METHODS Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS).					
36948263	1	35	dep	RELEVANCE	160:168	arg1	L.					195:196	M. chamomilla L.	181:196	M. chamomilla L.	181:196	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	4	36	theme	essential	993:1001	arg1	oils					1003:1006	M. chamomilla essential oils	979:1006	M. chamomilla essential oils (E.O)	979:1012	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	4	36	theme	essential	993:1001	arg1	E.O					1009:1011	E.O	1009:1011	E.O	1009:1011	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	11	37	theme	Oxidative	1922:1930	arg1	stress					1932:1937	Oxidative stress	1922:1937	Oxidative stress	1922:1937	Oxidative stress may trigger macrophages activation and elicit strong immune responses.					
36948263	0	38	theme	anti-inflammatory	31:47	arg1	effects					49:55	anti-inflammatory effects	31:55	anti-inflammatory effects involving human and murine macrophages	31:94	Chamomile essential oils exert anti-inflammatory effects involving human and murine macrophages: Evidence to support a therapeutic action.					
36948263	5	39	theme	aerial	1103:1108	arg1	parts					1110:1114	aerial parts	1103:1114	aerial parts of M. chamomilla	1103:1131	MATERIAL AND METHODS Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS).					
36948263	10	40	theme	p65	1837:1839	arg1	subunit					1882:1888	the critical transactivation subunit	1853:1888	the critical transactivation subunit for NF-κB transcription factor	1853:1919	These effects were correlated to a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor.					
36948263	10	40	theme	p65	1837:1839	arg1	activation					1841:1850	LPS-mediated p65 activation	1824:1850	LPS-mediated p65 activation	1824:1850	These effects were correlated to a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor.					
36948263	4	41	theme	chamomilla	806:815	arg1	plants					820:825	M. chamomilla L. plants	803:825	M. chamomilla L. plants collected three experimental field sites in the Molise	803:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	2	42	theme	herbal	323:328	arg1	It					303:304	It	303:304	It	303:304	It has been used as herbal remedies for thousands of years to treat several diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders.					
36948263	2	42	theme	herbal	323:328	arg1	remedies					330:337	herbal remedies	323:337	herbal remedies for thousands of years	323:360	It has been used as herbal remedies for thousands of years to treat several diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders.					
36948263	7	43	theme	sesquiterpenes	1478:1491	arg1	content					1452:1458	a high content	1445:1458	a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils	1445:1546	RESULTS The chemical analysis of the samples revealed the presence of a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils.					
36948263	1	44	contain	has	252:254	arg2	history					263:269	a long history	256:269	a long history of use in traditional medicine	256:300	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	1	44	contain	has	252:254	arg1	plant					216:220	an herbaceous plant	202:220	an herbaceous plant	202:220	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	1	44	contain	has	252:254	arg1	RELEVANCE					160:168	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile	139:178	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.)	139:197	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	5	45	theme	chromatography-mass	1183:1201	arg1	spectrometry					1203:1214	gas chromatography-mass spectrometry	1179:1214	gas chromatography-mass spectrometry (GC-MS)	1179:1222	MATERIAL AND METHODS Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS).					
36948263	5	45	theme	chromatography-mass	1183:1201	arg1	GC-MS					1217:1221	GC-MS	1217:1221	GC-MS	1217:1221	MATERIAL AND METHODS Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS).					
36948263	1	46	theme	Chamomile	170:178	arg1	plant					216:220	an herbaceous plant	202:220	an herbaceous plant	202:220	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	1	46	theme	Chamomile	170:178	arg1	RELEVANCE					160:168	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile	139:178	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.)	139:197	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	10	47	theme	critical	1857:1864	arg1	subunit					1882:1888	the critical transactivation subunit	1853:1888	the critical transactivation subunit for NF-κB transcription factor	1853:1919	These effects were correlated to a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor.					
36948263	10	47	theme	critical	1857:1864	arg1	activation					1841:1850	LPS-mediated p65 activation	1824:1850	LPS-mediated p65 activation	1824:1850	These effects were correlated to a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor.					
36948263	4	48	theme	essential	786:794	arg1	oil					796:798	the essential oil	782:798	the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise	782:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	3	49	dep	disease	650:656	arg1	maintenance					673:683	maintenance	673:683	maintenance	673:683	Chronic inflammation is involved in the pathogenesis of most infectious and non-infectious diseases and macrophages are considered the major cellular players that drive disease initiation and maintenance.					
36948263	3	49	dep	disease	650:656	arg1	initiation					658:667	initiation	658:667	initiation	658:667	Chronic inflammation is involved in the pathogenesis of most infectious and non-infectious diseases and macrophages are considered the major cellular players that drive disease initiation and maintenance.					
36948263	7	50	theme	content	1452:1458	arg1	presence					1433:1440	the presence	1429:1440	the presence of a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils	1429:1546	RESULTS The chemical analysis of the samples revealed the presence of a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils.					
36948263	13	51	theme	signaling	2264:2272	arg1	pathway					2274:2280	NRF2 signaling pathway	2259:2280	NRF2 signaling pathway	2259:2280	The antioxidant activity of these EOs was explained throughout the activation of NRF2 signaling pathway.					
36948263	4	52	theme	experimental	843:854	arg1	sites					862:866	three experimental field sites	837:866	three experimental field sites in the Molise	837:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	10	53	theme	NF-κB	1894:1898	arg1	factor					1914:1919	NF-κB transcription factor	1894:1919	NF-κB transcription factor	1894:1919	These effects were correlated to a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor.					
36948263	1	54	from	medicine	293:300	arg1	history					263:269	a long history	256:269	a long history of use in traditional medicine	256:300	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	15	55	theme	cells-mediate	2570:2582	arg1	response					2591:2598	CD4+ T cells-mediate immune response	2563:2598	CD4+ T cells-mediate immune response	2563:2598	CONCLUSIONS Our data describe for the first time that chamomile EOs exerted their anti-inflammatory and antioxidant activity by modulating macrophages and CD4+ T cells-mediate immune response.					
36948263	1	56	theme	long	258:261	arg1	history					263:269	a long history	256:269	a long history of use in traditional medicine	256:300	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	7	57	theme	samples	1412:1418	arg1	analysis					1396:1403	The chemical analysis	1383:1403	RESULTS The chemical analysis of the samples	1375:1418	RESULTS The chemical analysis of the samples revealed the presence of a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils.					
36948263	11	58	theme	immune	1992:1997	arg1	responses					1999:2007	strong immune responses	1985:2007	strong immune responses	1985:2007	Oxidative stress may trigger macrophages activation and elicit strong immune responses.					
36948263	4	59	theme	L.	817:818	arg1	plants					820:825	M. chamomilla L. plants	803:825	M. chamomilla L. plants collected three experimental field sites in the Molise	803:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	5	60	dep	MATERIAL	1015:1022	arg1	GC3					1078:1080	GC3	1078:1080	GC3	1078:1080	MATERIAL AND METHODS Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS).					
36948263	5	60	dep	MATERIAL	1015:1022	arg1	GC2					1070:1072	GC2	1070:1072	GC2	1070:1072	MATERIAL AND METHODS Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS).					
36948263	5	60	dep	MATERIAL	1015:1022	arg1	oils					1052:1055	Three essential oils	1036:1055	Three essential oils	1036:1055	MATERIAL AND METHODS Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS).					
36948263	5	60	dep	MATERIAL	1015:1022	arg1	GC1					1065:1067	called GC1	1058:1067	called GC1	1058:1067	MATERIAL AND METHODS Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS).					
36948263	3	61	theme	non-infectious	557:570	arg1	diseases					572:579	most infectious and non-infectious diseases	537:579	most infectious and non-infectious diseases	537:579	Chronic inflammation is involved in the pathogenesis of most infectious and non-infectious diseases and macrophages are considered the major cellular players that drive disease initiation and maintenance.					
36948263	6	62	dep	ability	1255:1261	arg1	functions					1364:1372	functions	1364:1372	functions	1364:1372	The Eos were tested for their ability to modulate pro-inflammatory murine macrophages and human peripheral blood mononuclear cells (PBMCs) functions.					
36948263	7	63	theme	chemical	1387:1394	arg1	analysis					1396:1403	The chemical analysis	1383:1403	RESULTS The chemical analysis of the samples	1375:1418	RESULTS The chemical analysis of the samples revealed the presence of a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils.					
36948263	6	64	theme	human	1315:1319	arg1	PBMCs					1357:1361	PBMCs	1357:1361	PBMCs	1357:1361	The Eos were tested for their ability to modulate pro-inflammatory murine macrophages and human peripheral blood mononuclear cells (PBMCs) functions.					
36948263	6	64	theme	human	1315:1319	arg1	cells					1350:1354	human peripheral blood mononuclear cells	1315:1354	human peripheral blood mononuclear cells (PBMCs)	1315:1362	The Eos were tested for their ability to modulate pro-inflammatory murine macrophages and human peripheral blood mononuclear cells (PBMCs) functions.					
36948263	14	65	theme	cell	2341:2344	arg1	activation					2346:2355	CD4+ T cell activation	2334:2355	CD4+ T cell activation which are also involved in inflammatory processes	2334:2405	Next, we demonstrated that EOs were able to reduce CD4+ T cell activation which are also involved in inflammatory processes.					
36948263	3	66	theme	infectious	542:551	arg1	diseases					572:579	most infectious and non-infectious diseases	537:579	most infectious and non-infectious diseases	537:579	Chronic inflammation is involved in the pathogenesis of most infectious and non-infectious diseases and macrophages are considered the major cellular players that drive disease initiation and maintenance.					
36948263	12	67	theme	HMOX-1	2099:2104	arg1	expression					2127:2136	HMOX-1 anti-oxidant enzymes expression	2099:2136	HMOX-1 anti-oxidant enzymes expression	2099:2136	Our study demonstrated that GC1, GC2 and GC3 were highly effective at increasing GCL and HMOX-1 anti-oxidant enzymes expression leading to the rapid scavenging of ROS.					
36948263	4	68	dep	mechanism	930:938	arg1	behind					940:945	behind	940:945	behind the anti-inflammatory effect of M. chamomilla essential oils (E.O)	940:1012	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	8	69	dep	GC1	1549:1551	arg1	oils					1576:1579	essential oils	1566:1579	essential oils	1566:1579	GC1, GC2 and GC3 essential oils significantly attenuated LPS/IFN-γ-induced inflammation by reducing M1 polarization.					
36948263	9	70	theme	significant	1690:1700	arg1	property					1720:1727	significant anti-inflammatory property	1690:1727	significant anti-inflammatory property	1690:1727	In details, they showed significant anti-inflammatory property by inhibiting NO, TNF-α and IL-6 production.					
36948263	12	71	theme	enzymes	2119:2125	arg1	expression					2127:2136	HMOX-1 anti-oxidant enzymes expression	2099:2136	HMOX-1 anti-oxidant enzymes expression	2099:2136	Our study demonstrated that GC1, GC2 and GC3 were highly effective at increasing GCL and HMOX-1 anti-oxidant enzymes expression leading to the rapid scavenging of ROS.					
36948263	6	72	theme	pro-inflammatory	1275:1290	arg1	macrophages					1299:1309	pro-inflammatory murine macrophages	1275:1309	pro-inflammatory murine macrophages	1275:1309	The Eos were tested for their ability to modulate pro-inflammatory murine macrophages and human peripheral blood mononuclear cells (PBMCs) functions.					
36948263	11	73	theme	macrophages	1951:1961	arg1	activation					1963:1972	macrophages activation	1951:1972	macrophages activation	1951:1972	Oxidative stress may trigger macrophages activation and elicit strong immune responses.					
36948263	15	74	theme	anti-inflammatory	2490:2506	arg1	activity					2524:2531	their anti-inflammatory and antioxidant activity	2484:2531	their anti-inflammatory and antioxidant activity	2484:2531	CONCLUSIONS Our data describe for the first time that chamomile EOs exerted their anti-inflammatory and antioxidant activity by modulating macrophages and CD4+ T cells-mediate immune response.					
36948263	15	75	dep	CONCLUSIONS	2408:2418	arg1	describe					2429:2436	describe	2429:2436	describe for the first time that chamomile EOs exerted their anti-inflammatory and antioxidant activity by modulating macrophages and CD4+ T cells-mediate immune response	2429:2598	CONCLUSIONS Our data describe for the first time that chamomile EOs exerted their anti-inflammatory and antioxidant activity by modulating macrophages and CD4+ T cells-mediate immune response.					
36948263	14	76	theme	T	2339:2339	arg1	activation					2346:2355	CD4+ T cell activation	2334:2355	CD4+ T cell activation which are also involved in inflammatory processes	2334:2405	Next, we demonstrated that EOs were able to reduce CD4+ T cell activation which are also involved in inflammatory processes.					
36948263	10	77	theme	LPS-mediated	1824:1835	arg1	subunit					1882:1888	the critical transactivation subunit	1853:1888	the critical transactivation subunit for NF-κB transcription factor	1853:1919	These effects were correlated to a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor.					
36948263	10	77	theme	LPS-mediated	1824:1835	arg1	activation					1841:1850	LPS-mediated p65 activation	1824:1850	LPS-mediated p65 activation	1824:1850	These effects were correlated to a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor.					
36948263	5	78	theme	essential	1042:1050	arg1	oils					1052:1055	Three essential oils	1036:1055	Three essential oils	1036:1055	MATERIAL AND METHODS Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS).					
36948263	11	79	theme	strong	1985:1990	arg1	responses					1999:2007	strong immune responses	1985:2007	strong immune responses	1985:2007	Oxidative stress may trigger macrophages activation and elicit strong immune responses.					
36948263	15	80	theme	chamomile	2462:2470	arg1	EOs					2472:2474	chamomile EOs	2462:2474	chamomile EOs	2462:2474	CONCLUSIONS Our data describe for the first time that chamomile EOs exerted their anti-inflammatory and antioxidant activity by modulating macrophages and CD4+ T cells-mediate immune response.					
36948263	13	81	theme	NRF2	2259:2262	arg1	pathway					2274:2280	NRF2 signaling pathway	2259:2280	NRF2 signaling pathway	2259:2280	The antioxidant activity of these EOs was explained throughout the activation of NRF2 signaling pathway.					
36948263	7	82	theme	oils	1543:1546	arg1	half					1524:1527	the half	1520:1527	the half of the entire oils	1520:1546	RESULTS The chemical analysis of the samples revealed the presence of a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils.					
36948263	4	83	theme	anti-inflammatory	951:967	arg1	effect					969:974	the anti-inflammatory effect	947:974	the anti-inflammatory effect of M. chamomilla essential oils (E.O)	947:1012	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	1	84	theme	family	227:232	arg1	Astereaceae					234:244	family Astereaceae	227:244	family Astereaceae	227:244	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	4	85	from	sites	862:866	arg1	Molise					875:880	the Molise	871:880	the Molise	871:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	13	86	theme	pathway	2274:2280	arg1	activation					2245:2254	the activation	2241:2254	the activation of NRF2 signaling pathway	2241:2280	The antioxidant activity of these EOs was explained throughout the activation of NRF2 signaling pathway.					
36948263	2	87	theme	liver	464:468	arg1	disorders					470:478	liver disorders	464:478	liver disorders	464:478	It has been used as herbal remedies for thousands of years to treat several diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders.					
36948263	2	87	theme	liver	464:468	arg1	infections					399:408	infections	399:408	infections	399:408	It has been used as herbal remedies for thousands of years to treat several diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders.					
36948263	4	88	theme	STUDY	697:701	arg1	AIM					686:688	AIM	686:688	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise	686:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	15	89	theme	CD4+	2563:2566	arg1	response					2591:2598	CD4+ T cells-mediate immune response	2563:2598	CD4+ T cells-mediate immune response	2563:2598	CONCLUSIONS Our data describe for the first time that chamomile EOs exerted their anti-inflammatory and antioxidant activity by modulating macrophages and CD4+ T cells-mediate immune response.					
36948263	4	90	theme	pharmacological	914:928	arg1	mechanism					930:938	the pharmacological mechanism	910:938	the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O)	910:1012	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	6	91	theme	blood	1332:1336	arg1	PBMCs					1357:1361	PBMCs	1357:1361	PBMCs	1357:1361	The Eos were tested for their ability to modulate pro-inflammatory murine macrophages and human peripheral blood mononuclear cells (PBMCs) functions.					
36948263	6	91	theme	blood	1332:1336	arg1	cells					1350:1354	human peripheral blood mononuclear cells	1315:1354	human peripheral blood mononuclear cells (PBMCs)	1315:1362	The Eos were tested for their ability to modulate pro-inflammatory murine macrophages and human peripheral blood mononuclear cells (PBMCs) functions.					
36948263	4	92	from	variation	745:753	arg1	profile					771:777	the chemical profile	758:777	the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise	758:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	0	93	theme	essential	10:18	arg1	oils					20:23	Chamomile essential oils	0:23	Chamomile essential oils	0:23	Chamomile essential oils exert anti-inflammatory effects involving human and murine macrophages: Evidence to support a therapeutic action.					
36948263	2	94	theme	years	356:360	arg1	thousands					343:351	thousands	343:351	thousands of years	343:360	It has been used as herbal remedies for thousands of years to treat several diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders.					
36948263	1	95	theme	use	274:276	arg1	history					263:269	a long history	256:269	a long history of use in traditional medicine	256:300	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	15	96	theme	antioxidant	2512:2522	arg1	activity					2524:2531	their anti-inflammatory and antioxidant activity	2484:2531	their anti-inflammatory and antioxidant activity	2484:2531	CONCLUSIONS Our data describe for the first time that chamomile EOs exerted their anti-inflammatory and antioxidant activity by modulating macrophages and CD4+ T cells-mediate immune response.					
36948263	1	97	theme	traditional	281:291	arg1	medicine					293:300	traditional medicine	281:300	traditional medicine	281:300	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	0	98	dep	human	67:71	arg1	macrophages					84:94	macrophages	84:94	macrophages	84:94	Chamomile essential oils exert anti-inflammatory effects involving human and murine macrophages: Evidence to support a therapeutic action.					
36948263	4	99	theme	chamomilla	982:991	arg1	oils					1003:1006	M. chamomilla essential oils	979:1006	M. chamomilla essential oils (E.O)	979:1012	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	4	99	theme	chamomilla	982:991	arg1	E.O					1009:1011	E.O	1009:1011	E.O	1009:1011	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	10	100	theme	activation	1841:1850	arg1	suppression					1809:1819	a suppression	1807:1819	a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor	1807:1919	These effects were correlated to a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor.					
36948263	4	101	theme	oil	796:798	arg1	profile					771:777	the chemical profile	758:777	the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise	758:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	13	102	theme	EOs	2212:2214	arg1	activity					2194:2201	The antioxidant activity	2178:2201	The antioxidant activity of these EOs	2178:2214	The antioxidant activity of these EOs was explained throughout the activation of NRF2 signaling pathway.					
36948263	4	103	theme	oils	1003:1006	arg1	effect					969:974	the anti-inflammatory effect	947:974	the anti-inflammatory effect of M. chamomilla essential oils (E.O)	947:1012	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	4	104	theme	M.	803:804	arg1	plants					820:825	M. chamomilla L. plants	803:825	M. chamomilla L. plants collected three experimental field sites in the Molise	803:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	3	105	theme	Chronic	481:487	arg1	inflammation					489:500	Chronic inflammation	481:500	Chronic inflammation	481:500	Chronic inflammation is involved in the pathogenesis of most infectious and non-infectious diseases and macrophages are considered the major cellular players that drive disease initiation and maintenance.					
36948263	4	106	dep	AIM	686:688	arg1	was					725:727	was	725:727	was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise	725:880	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla L. plants collected three experimental field sites in the Molise, Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils (E.O).					
36948263	10	107	theme	transactivation	1866:1880	arg1	subunit					1882:1888	the critical transactivation subunit	1853:1888	the critical transactivation subunit for NF-κB transcription factor	1853:1919	These effects were correlated to a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor.					
36948263	10	107	theme	transactivation	1866:1880	arg1	activation					1841:1850	LPS-mediated p65 activation	1824:1850	LPS-mediated p65 activation	1824:1850	These effects were correlated to a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor.					
36948263	8	108	theme	M1	1649:1650	arg1	polarization					1652:1663	M1 polarization	1649:1663	M1 polarization	1649:1663	GC1, GC2 and GC3 essential oils significantly attenuated LPS/IFN-γ-induced inflammation by reducing M1 polarization.					
36948263	13	109	theme	antioxidant	2182:2192	arg1	activity					2194:2201	The antioxidant activity	2178:2201	The antioxidant activity of these EOs	2178:2214	The antioxidant activity of these EOs was explained throughout the activation of NRF2 signaling pathway.					
36948263	7	110	theme	oxygenated	1467:1476	arg1	sesquiterpenes					1478:1491	the oxygenated sesquiterpenes	1463:1491	the oxygenated sesquiterpenes that represented more than the half of the entire oils	1463:1546	RESULTS The chemical analysis of the samples revealed the presence of a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils.					
36948263	1	111	from	history	263:269	arg1	medicine					293:300	traditional medicine	281:300	traditional medicine	281:300	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine.					
36948263	8	112	theme	essential	1566:1574	arg1	oils					1576:1579	essential oils	1566:1579	essential oils	1566:1579	GC1, GC2 and GC3 essential oils significantly attenuated LPS/IFN-γ-induced inflammation by reducing M1 polarization.					
36948263	12	113	theme	ROS	2173:2175	arg1	scavenging					2159:2168	the rapid scavenging	2149:2168	the rapid scavenging of ROS	2149:2175	Our study demonstrated that GC1, GC2 and GC3 were highly effective at increasing GCL and HMOX-1 anti-oxidant enzymes expression leading to the rapid scavenging of ROS.					
36948263	15	114	theme	immune	2584:2589	arg1	response					2591:2598	CD4+ T cells-mediate immune response	2563:2598	CD4+ T cells-mediate immune response	2563:2598	CONCLUSIONS Our data describe for the first time that chamomile EOs exerted their anti-inflammatory and antioxidant activity by modulating macrophages and CD4+ T cells-mediate immune response.					
37080407	6	0	theme	CC	1083:1084	arg1	film					1087:1090	collagen/chitosan (CC) film	1064:1090	collagen/chitosan (CC) film	1064:1090	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	8	1	theme	blocking	1549:1556	arg1	capacity					1558:1565	the best mechanical property, thermostability, UV light and water vapor blocking capacity	1477:1565	the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity	1436:1565	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	6	2	theme	lower	1229:1233	arg1	2.64-2.98 × 10-12 g m-1 s-1 Pa-1					1268:1299	2.64-2.98 × 10-12 g m-1 s-1 Pa-1	1268:1299	2.64-2.98 × 10-12 g m-1 s-1 Pa-1	1268:1299	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	6	2	theme	lower	1229:1233	arg1	values					1260:1265	higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values	1123:1265	higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1)	1123:1300	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	6	3	theme	water	1235:1239	arg1	2.64-2.98 × 10-12 g m-1 s-1 Pa-1					1268:1299	2.64-2.98 × 10-12 g m-1 s-1 Pa-1	1268:1299	2.64-2.98 × 10-12 g m-1 s-1 Pa-1	1268:1299	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	6	3	theme	water	1235:1239	arg1	values					1260:1265	higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values	1123:1265	higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1)	1123:1300	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	7	4	theme	films	1357:1361	arg1	properties					1307:1316	The properties	1303:1316	The properties of collagen/ phenolic acids-g-chitosan films	1303:1361	The properties of collagen/ phenolic acids-g-chitosan films were greatly affected by the type of phenolic acid grafted.					
37080407	6	5	theme	collagen/chitosan	1064:1080	arg1	film					1087:1090	collagen/chitosan (CC) film	1064:1090	collagen/chitosan (CC) film	1064:1090	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	4	6	dep	properties	715:724	arg1	the					702:704	the	702:704	the	702:704	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	6	7	dep	higher	1169:1174	arg1	69.4-71.2 °C					1210:1221	69.4-71.2 °C	1210:1221	69.4-71.2 °C	1210:1221	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	6	7	dep	higher	1169:1174	arg1	temperature					1197:1207	thermal denaturation temperature	1176:1207	thermal denaturation temperature (69.4-71.2 °C)	1176:1222	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	8	8	theme	best	1440:1443	arg1	property					1457:1464	the best antioxidant property	1436:1464	the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity	1436:1565	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	7	9	theme	collagen/	1321:1329	arg1	films					1357:1361	collagen/ phenolic acids-g-chitosan films	1321:1361	collagen/ phenolic acids-g-chitosan films	1321:1361	The properties of collagen/ phenolic acids-g-chitosan films were greatly affected by the type of phenolic acid grafted.					
37080407	7	10	theme	phenolic	1331:1338	arg1	films					1357:1361	collagen/ phenolic acids-g-chitosan films	1321:1361	collagen/ phenolic acids-g-chitosan films	1321:1361	The properties of collagen/ phenolic acids-g-chitosan films were greatly affected by the type of phenolic acid grafted.					
37080407	5	11	theme	due	1002:1004	arg1	capacity					951:958	significantly improved UV light blocking capacity	910:958	significantly improved UV light blocking capacity	910:958	The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid.					
37080407	3	12	theme	antioxidant	539:549	arg1	activities					569:578	the antioxidant and antimicrobial activities	535:578	the antioxidant and antimicrobial activities of collagen films	535:596	In this work, phenolic acid-grafted-chitosan was blended with collagen to improve the antioxidant and antimicrobial activities of collagen films.					
37080407	7	13	theme	acid	1409:1412	arg1	type					1392:1395	the type	1388:1395	the type of phenolic acid grafted	1388:1420	The properties of collagen/ phenolic acids-g-chitosan films were greatly affected by the type of phenolic acid grafted.					
37080407	0	14	theme	food	87:90	arg1	packaging					92:100	food packaging	87:100	food packaging	87:100	Development of composite film based on collagen and phenolic acid-grafted chitosan for food packaging.					
37080407	8	15	theme	best	1481:1484	arg1	capacity					1558:1565	the best mechanical property, thermostability, UV light and water vapor blocking capacity	1477:1565	the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity	1436:1565	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	6	16	theme	mechanical	1130:1139	arg1	69.08-73.79 MPa					1151:1165	69.08-73.79 MPa	1151:1165	69.08-73.79 MPa	1151:1165	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	6	16	theme	mechanical	1130:1139	arg1	strength					1141:1148	mechanical strength	1130:1148	mechanical strength (69.08-73.79 MPa)	1130:1166	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	5	17	theme	UV	933:934	arg1	capacity					951:958	significantly improved UV light blocking capacity	910:958	significantly improved UV light blocking capacity	910:958	The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid.					
37080407	1	18	theme	biodegradability	262:277	arg1	advantages					248:257	advantages	248:257	advantages of biodegradability, high mechanical strength and good water resistance	248:329	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	5	19	theme	light	936:940	arg1	capacity					951:958	significantly improved UV light blocking capacity	910:958	significantly improved UV light blocking capacity	910:958	The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid.					
37080407	4	20	dep	acids-g-chitosan	777:792	arg1	CGC					795:797	CGC	795:797	CGC	795:797	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	4	20	dep	acids-g-chitosan	777:792	arg1	CCC					808:810	CCC	808:810	CCC	808:810	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	4	20	dep	acids-g-chitosan	777:792	arg1	CFC					800:802	CFC	800:802	CFC	800:802	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	4	21	theme	Gallic	599:604	arg1	GA					612:613	GA	612:613	GA	612:613	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	4	21	theme	Gallic	599:604	arg1	acid					606:609	Gallic acid	599:609	Gallic acid (GA)	599:614	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	8	22	theme	property	1497:1504	arg1	capacity					1558:1565	the best mechanical property, thermostability, UV light and water vapor blocking capacity	1477:1565	the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity	1436:1565	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	1	23	with	protein	123:129	arg1	structure					151:159	triple-helical structure	136:159	triple-helical structure	136:159	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	4	24	theme	functional	730:739	arg1	activities					741:750	functional activities	730:750	functional activities	730:750	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	8	25	theme	vapor	1543:1547	arg1	capacity					1558:1565	the best mechanical property, thermostability, UV light and water vapor blocking capacity	1477:1565	the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity	1436:1565	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	2	26	theme	packaging	442:450	arg1	field					426:430	the field	422:430	the field of active packaging	422:450	However, collagen films lack functional activities, which may limit their applications in the field of active packaging.					
37080407	8	27	theme	mechanical	1486:1495	arg1	property					1497:1504	mechanical property	1486:1504	mechanical property	1486:1504	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	4	28	theme	films	813:817	arg1	properties					715:724	physical properties	706:724	physical properties	706:724	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	4	28	theme	films	813:817	arg1	activities					741:750	functional activities	730:750	functional activities	730:750	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	8	29	theme	thermostability	1507:1521	arg1	capacity					1558:1565	the best mechanical property, thermostability, UV light and water vapor blocking capacity	1477:1565	the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity	1436:1565	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	1	30	theme	good	167:170	arg1	substrate					185:193	a good film-forming substrate	165:193	a good film-forming substrate for food packaging films	165:218	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	1	30	theme	good	167:170	arg1	Collagen					103:110	Collagen	103:110	Collagen	103:110	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	2	31	theme	active	435:440	arg1	packaging					442:450	active packaging	435:450	active packaging	435:450	However, collagen films lack functional activities, which may limit their applications in the field of active packaging.					
37080407	7	32	theme	phenolic	1400:1407	arg1	acid					1409:1412	phenolic acid	1400:1412	phenolic acid grafted	1400:1420	The properties of collagen/ phenolic acids-g-chitosan films were greatly affected by the type of phenolic acid grafted.					
37080407	6	33	theme	denaturation	1184:1195	arg1	69.4-71.2 °C					1210:1221	69.4-71.2 °C	1210:1221	69.4-71.2 °C	1210:1221	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	6	33	theme	denaturation	1184:1195	arg1	temperature					1197:1207	thermal denaturation temperature	1176:1207	thermal denaturation temperature (69.4-71.2 °C)	1176:1222	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	3	34	theme	collagen	583:590	arg1	films					592:596	collagen films	583:596	collagen films	583:596	In this work, phenolic acid-grafted-chitosan was blended with collagen to improve the antioxidant and antimicrobial activities of collagen films.					
37080407	1	35	theme	film-forming	172:183	arg1	substrate					185:193	a good film-forming substrate	165:193	a good film-forming substrate for food packaging films	165:218	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	1	35	theme	film-forming	172:183	arg1	Collagen					103:110	Collagen	103:110	Collagen	103:110	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	0	36	theme	film	25:28	arg1	Development					0:10	Development	0:10	Development of composite film	0:28	Development of composite film based on collagen and phenolic acid-grafted chitosan for food packaging.					
37080407	1	37	theme	high	280:283	arg1	strength					296:303	high mechanical strength	280:303	high mechanical strength	280:303	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	5	38	theme	color	889:893	arg1	degrees					871:877	varying degrees	863:877	varying degrees of yellow color	863:893	The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid.					
37080407	3	39	theme	antimicrobial	555:567	arg1	activities					569:578	the antioxidant and antimicrobial activities	535:578	the antioxidant and antimicrobial activities of collagen films	535:596	In this work, phenolic acid-grafted-chitosan was blended with collagen to improve the antioxidant and antimicrobial activities of collagen films.					
37080407	6	40	theme	thermal	1176:1182	arg1	69.4-71.2 °C					1210:1221	69.4-71.2 °C	1210:1221	69.4-71.2 °C	1210:1221	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	6	40	theme	thermal	1176:1182	arg1	temperature					1197:1207	thermal denaturation temperature	1176:1207	thermal denaturation temperature (69.4-71.2 °C)	1176:1222	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	0	41	theme	composite	15:23	arg1	film					25:28	composite film	15:28	composite film	15:28	Development of composite film based on collagen and phenolic acid-grafted chitosan for food packaging.					
37080407	1	42	theme	mechanical	285:294	arg1	strength					296:303	high mechanical strength	280:303	high mechanical strength	280:303	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	5	43	theme	acid	1034:1037	arg1	function					1013:1020	the function	1009:1020	the function of phenolic acid	1009:1037	The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid.					
37080407	5	44	theme	varying	863:869	arg1	degrees					871:877	varying degrees	863:877	varying degrees of yellow color	863:893	The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid.					
37080407	8	45	theme	UV	1524:1525	arg1	light					1527:1531	UV light	1524:1531	UV light	1524:1531	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	4	46	theme	ferulic	617:623	arg1	FA					631:632	FA	631:632	FA	631:632	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	4	46	theme	ferulic	617:623	arg1	acid					625:628	ferulic acid	617:628	ferulic acid (FA)	617:633	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	1	47	theme	strength	296:303	arg1	advantages					248:257	advantages	248:257	advantages of biodegradability, high mechanical strength and good water resistance	248:329	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	6	48	dep	higher	1123:1128	arg1	69.08-73.79 MPa					1151:1165	69.08-73.79 MPa	1151:1165	69.08-73.79 MPa	1151:1165	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	6	48	dep	higher	1123:1128	arg1	strength					1141:1148	mechanical strength	1130:1148	mechanical strength (69.08-73.79 MPa)	1130:1166	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	5	49	theme	antimicrobial	977:989	arg1	properties					991:1000	antimicrobial properties	977:1000	antimicrobial properties	977:1000	The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid.					
37080407	4	50	theme	caffeic	639:645	arg1	CA					653:654	CA	653:654	CA	653:654	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	4	50	theme	caffeic	639:645	arg1	acid					647:650	caffeic acid	639:650	caffeic acid (CA)	639:655	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	1	51	theme	food	199:202	arg1	films					214:218	food packaging films	199:218	food packaging films	199:218	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	6	52	theme	higher	1169:1174	arg1	2.64-2.98 × 10-12 g m-1 s-1 Pa-1					1268:1299	2.64-2.98 × 10-12 g m-1 s-1 Pa-1	1268:1299	2.64-2.98 × 10-12 g m-1 s-1 Pa-1	1268:1299	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	6	52	theme	higher	1169:1174	arg1	values					1260:1265	higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values	1123:1265	higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1)	1123:1300	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	1	53	theme	packaging	204:212	arg1	films					214:218	food packaging films	199:218	food packaging films	199:218	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	1	54	theme	good	309:312	arg1	resistance					320:329	good water resistance	309:329	good water resistance	309:329	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	8	55	theme	CGC	1423:1425	arg1	film					1427:1430	CGC film	1423:1430	CGC film	1423:1430	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	3	56	theme	films	592:596	arg1	activities					569:578	the antioxidant and antimicrobial activities	535:578	the antioxidant and antimicrobial activities of collagen films	535:596	In this work, phenolic acid-grafted-chitosan was blended with collagen to improve the antioxidant and antimicrobial activities of collagen films.					
37080407	2	57	theme	collagen	341:348	arg1	films					350:354	collagen films	341:354	collagen films	341:354	However, collagen films lack functional activities, which may limit their applications in the field of active packaging.					
37080407	1	58	theme	water	314:318	arg1	resistance					320:329	good water resistance	309:329	good water resistance	309:329	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	6	59	theme	permeability	1247:1258	arg1	2.64-2.98 × 10-12 g m-1 s-1 Pa-1					1268:1299	2.64-2.98 × 10-12 g m-1 s-1 Pa-1	1268:1299	2.64-2.98 × 10-12 g m-1 s-1 Pa-1	1268:1299	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	6	59	theme	permeability	1247:1258	arg1	values					1260:1265	higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values	1123:1265	higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1)	1123:1300	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	7	60	theme	acids-g-chitosan	1340:1355	arg1	films					1357:1361	collagen/ phenolic acids-g-chitosan films	1321:1361	collagen/ phenolic acids-g-chitosan films	1321:1361	The properties of collagen/ phenolic acids-g-chitosan films were greatly affected by the type of phenolic acid grafted.					
37080407	8	61	theme	water	1537:1541	arg1	vapor					1543:1547	water vapor	1537:1547	water vapor	1537:1547	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	1	62	theme	resistance	320:329	arg1	advantages					248:257	advantages	248:257	advantages of biodegradability, high mechanical strength and good water resistance	248:329	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	6	63	theme	vapor	1241:1245	arg1	2.64-2.98 × 10-12 g m-1 s-1 Pa-1					1268:1299	2.64-2.98 × 10-12 g m-1 s-1 Pa-1	1268:1299	2.64-2.98 × 10-12 g m-1 s-1 Pa-1	1268:1299	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	6	63	theme	vapor	1241:1245	arg1	values					1260:1265	higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values	1123:1265	higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1)	1123:1300	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	1	64	theme	fibrous	115:121	arg1	protein					123:129	a fibrous protein	113:129	a fibrous protein with triple-helical structure	113:159	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	1	64	theme	fibrous	115:121	arg1	Collagen					103:110	Collagen	103:110	Collagen	103:110	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	4	65	theme	physical	706:713	arg1	properties					715:724	physical properties	706:724	physical properties	706:724	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	5	66	theme	blocking	942:949	arg1	capacity					951:958	significantly improved UV light blocking capacity	910:958	significantly improved UV light blocking capacity	910:958	The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid.					
37080407	8	67	theme	antioxidant	1445:1455	arg1	property					1457:1464	the best antioxidant property	1436:1464	the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity	1436:1565	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	5	68	theme	improved	924:931	arg1	capacity					951:958	significantly improved UV light blocking capacity	910:958	significantly improved UV light blocking capacity	910:958	The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid.					
37080407	2	69	theme	functional	361:370	arg1	activities					372:381	functional activities	361:381	functional activities	361:381	However, collagen films lack functional activities, which may limit their applications in the field of active packaging.					
37080407	8	70	contain	had	1432:1434	arg2	property					1457:1464	the best antioxidant property	1436:1464	the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity	1436:1565	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	8	70	contain	had	1432:1434	arg1	film					1427:1430	CGC film	1423:1430	CGC film	1423:1430	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	8	70	contain	had	1432:1434	arg2	capacity					1558:1565	the best mechanical property, thermostability, UV light and water vapor blocking capacity	1477:1565	the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity	1436:1565	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
37080407	5	71	theme	yellow	882:887	arg1	color					889:893	yellow color	882:893	yellow color	882:893	The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid.					
37080407	0	72	theme	acid-grafted	61:72	arg1	chitosan					74:81	phenolic acid-grafted chitosan	52:81	phenolic acid-grafted chitosan for food packaging	52:100	Development of composite film based on collagen and phenolic acid-grafted chitosan for food packaging.					
37080407	5	73	theme	phenolic	1025:1032	arg1	acid					1034:1037	phenolic acid	1025:1037	phenolic acid	1025:1037	The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid.					
37080407	4	74	theme	acids-g-chitosan	777:792	arg1	films					813:817	the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films	755:817	the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films	755:817	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	5	75	theme	prepared	838:845	arg1	films					847:851	The prepared films	834:851	The prepared films	834:851	The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid.					
37080407	3	76	theme	phenolic	467:474	arg1	acid-grafted-chitosan					476:496	phenolic acid-grafted-chitosan	467:496	phenolic acid-grafted-chitosan	467:496	In this work, phenolic acid-grafted-chitosan was blended with collagen to improve the antioxidant and antimicrobial activities of collagen films.					
37080407	1	77	theme	collagen	228:235	arg1	films					237:241	collagen films	228:241	collagen films	228:241	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	0	78	theme	phenolic	52:59	arg1	chitosan					74:81	phenolic acid-grafted chitosan	52:81	phenolic acid-grafted chitosan for food packaging	52:100	Development of composite film based on collagen and phenolic acid-grafted chitosan for food packaging.					
37080407	4	79	theme	collagen/phenolic	759:775	arg1	acids-g-chitosan					777:792	the collagen/phenolic acids-g-chitosan	755:792	the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films	755:817	Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared.					
37080407	6	80	theme	higher	1123:1128	arg1	2.64-2.98 × 10-12 g m-1 s-1 Pa-1					1268:1299	2.64-2.98 × 10-12 g m-1 s-1 Pa-1	1268:1299	2.64-2.98 × 10-12 g m-1 s-1 Pa-1	1268:1299	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	6	80	theme	higher	1123:1128	arg1	values					1260:1265	higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values	1123:1265	higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1)	1123:1300	Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10-12 g m-1 s-1 Pa-1).					
37080407	1	81	theme	triple-helical	136:149	arg1	structure					151:159	triple-helical structure	136:159	triple-helical structure	136:159	Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance.					
37080407	8	82	theme	light	1527:1531	arg1	capacity					1558:1565	the best mechanical property, thermostability, UV light and water vapor blocking capacity	1477:1565	the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity	1436:1565	CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.					
36470349	3	0	theme	sorbent	510:516	arg1	dose					518:521	sorbent dose	510:521	sorbent dose	510:521	Batch removal of chromium (Cr) was optimized with respect to solution pH, batch stirring time, sorbent dose, initial chromium concentration and temperature.					
36470349	2	1	from	effect	347:352	arg1	process					389:395	adsorption process	378:395	adsorption process	378:395	The effect of process parameter on adsorption process was investigated.					
36470349	11	2	theme	pinnata	1473:1479	arg1	modification					1450:1461	Surface modification	1442:1461	Surface modification of Azolla pinnata with chitosan and iron oxide	1442:1508	Surface modification of Azolla pinnata with chitosan and iron oxide improved the ability of Azolla in the adsorption of chromium from aqueous media.					
36470349	9	3	theme	removal	1214:1220	arg1	efficiency					1222:1231	removal efficiency	1214:1231	removal efficiency attained	1214:1240	Reusability studies have confirmed that removal efficiency attained was 85% after completion of five adsorption-desorption cycles.					
36470349	6	4	theme	adsorption	904:913	arg1	R2 = 0.996					925:934	R2 = 0.996	925:934	R2 = 0.996	925:934	The adsorption data fitted well with Langmuir adsorption isotherm (R2 = 0.996) confirming mono layer sorption and the maximum uptake was found to be 294.12 mg/g.					
36470349	6	4	theme	adsorption	904:913	arg1	isotherm					915:922	Langmuir adsorption isotherm	895:922	Langmuir adsorption isotherm (R2 = 0.996) confirming mono layer sorption	895:966	The adsorption data fitted well with Langmuir adsorption isotherm (R2 = 0.996) confirming mono layer sorption and the maximum uptake was found to be 294.12 mg/g.					
36470349	3	5	theme	chromium	532:539	arg1	concentration					541:553	initial chromium concentration	524:553	initial chromium concentration	524:553	Batch removal of chromium (Cr) was optimized with respect to solution pH, batch stirring time, sorbent dose, initial chromium concentration and temperature.					
36470349	6	6	theme	layer	953:957	arg1	sorption					959:966	mono layer sorption	948:966	mono layer sorption	948:966	The adsorption data fitted well with Langmuir adsorption isotherm (R2 = 0.996) confirming mono layer sorption and the maximum uptake was found to be 294.12 mg/g.					
36470349	1	7	theme	novel	190:194	arg1	nanocomposite					203:215	a novel porous nanocomposite	188:215	a novel porous nanocomposite	188:215	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	1	7	theme	novel	190:194	arg1	nanocomposite					262:274	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	225:274	namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite	218:274	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	5	8	theme	nanocomposite	843:855	arg1	presence					762:769	the presence	758:769	the presence of characteristic functional groups and porous structure of synthesized nanocomposite	758:855	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	7	9	theme	order	1069:1073	arg1	R2 = 0.997					1082:1091	R2 = 0.997	1082:1091	R2 = 0.997	1082:1091	The adsorption was found to follow pseudo second order model (R2 = 0.997).					
36470349	7	9	theme	order	1069:1073	arg1	model					1075:1079	pseudo second order model	1055:1079	pseudo second order model (R2 = 0.997)	1055:1092	The adsorption was found to follow pseudo second order model (R2 = 0.997).					
36470349	5	10	theme	structure	818:826	arg1	presence					762:769	the presence	758:769	the presence of characteristic functional groups and porous structure of synthesized nanocomposite	758:855	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	12	11	theme	synthesis	1622:1630	arg1	effects					1604:1610	The combined effects	1591:1610	The combined effects	1591:1610	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	7	12	theme	pseudo	1055:1060	arg1	R2 = 0.997					1082:1091	R2 = 0.997	1082:1091	R2 = 0.997	1082:1091	The adsorption was found to follow pseudo second order model (R2 = 0.997).					
36470349	7	12	theme	pseudo	1055:1060	arg1	model					1075:1079	pseudo second order model	1055:1079	pseudo second order model (R2 = 0.997)	1055:1092	The adsorption was found to follow pseudo second order model (R2 = 0.997).					
36470349	9	13	theme	adsorption-desorption	1275:1295	arg1	cycles					1297:1302	five adsorption-desorption cycles	1270:1302	five adsorption-desorption cycles	1270:1302	Reusability studies have confirmed that removal efficiency attained was 85% after completion of five adsorption-desorption cycles.					
36470349	6	14	theme	mono	948:951	arg1	sorption					959:966	mono layer sorption	948:966	mono layer sorption	948:966	The adsorption data fitted well with Langmuir adsorption isotherm (R2 = 0.996) confirming mono layer sorption and the maximum uptake was found to be 294.12 mg/g.					
36470349	5	15	theme	electron	691:698	arg1	SEM					712:714	SEM	712:714	SEM	712:714	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	5	15	theme	electron	691:698	arg1	microscope					700:709	scanning electron microscope	682:709	scanning electron microscope (SEM) analysis of the nano composite	682:746	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	12	16	theme	combined	1595:1602	arg1	effects					1604:1610	The combined effects	1591:1610	The combined effects	1591:1610	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	11	17	theme	Azolla	1534:1539	arg1	ability					1523:1529	the ability	1519:1529	the ability of Azolla	1519:1539	Surface modification of Azolla pinnata with chitosan and iron oxide improved the ability of Azolla in the adsorption of chromium from aqueous media.					
36470349	11	18	from	media	1584:1588	arg1	adsorption					1548:1557	the adsorption	1544:1557	the adsorption of chromium from aqueous media	1544:1588	Surface modification of Azolla pinnata with chitosan and iron oxide improved the ability of Azolla in the adsorption of chromium from aqueous media.					
36470349	11	19	theme	aqueous	1576:1582	arg1	media					1584:1588	aqueous media	1576:1588	aqueous media	1576:1588	Surface modification of Azolla pinnata with chitosan and iron oxide improved the ability of Azolla in the adsorption of chromium from aqueous media.					
36470349	0	20	theme	chromium	100:107	arg1	removal					89:95	removal	89:95	removal of chromium	89:107	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	12	21	theme	pinnata	1731:1737	arg1	adsorbent					1764:1772	adsorbent	1764:1772	adsorbent	1764:1772	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	12	21	theme	pinnata	1731:1737	arg1	nanocomposite					1739:1751	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	1702:1751	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	1702:1751	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	11	22	theme	iron	1499:1502	arg1	oxide					1504:1508	iron oxide	1499:1508	iron oxide	1499:1508	Surface modification of Azolla pinnata with chitosan and iron oxide improved the ability of Azolla in the adsorption of chromium from aqueous media.					
36470349	0	23	theme	Parametric	111:120	arg1	characterization					139:154	surface characterization	131:154	surface characterization	131:154	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	0	23	theme	Parametric	111:120	arg1	kinetics					160:167	kinetics	160:167	kinetics	160:167	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	0	23	theme	Parametric	111:120	arg1	effects					122:128	Parametric effects	111:128	Parametric effects	111:128	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	3	24	theme	Batch	415:419	arg1	removal					421:427	Batch removal	415:427	Batch removal of chromium (Cr)	415:444	Batch removal of chromium (Cr) was optimized with respect to solution pH, batch stirring time, sorbent dose, initial chromium concentration and temperature.					
36470349	5	25	theme	porous	811:816	arg1	structure					818:826	porous structure	811:826	porous structure	811:826	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	10	26	theme	Electrostatic	1305:1317	arg1	attraction					1319:1328	Electrostatic attraction	1305:1328	Electrostatic attraction	1305:1328	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	26	theme	Electrostatic	1305:1317	arg1	exchange					1335:1342	ion exchange	1331:1342	ion exchange	1331:1342	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	26	theme	Electrostatic	1305:1317	arg1	bonding					1358:1364	coordination bonding	1345:1364	coordination bonding	1345:1364	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	26	theme	Electrostatic	1305:1317	arg1	reduction					1370:1378	reduction	1370:1378	reduction	1370:1378	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	26	theme	Electrostatic	1305:1317	arg1	mechanisms					1394:1403	the major mechanisms	1384:1403	the major mechanisms responsible for removal of chromium	1384:1439	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	5	27	dep	infrared	649:656	arg1	FTIR					659:662	FTIR	659:662	FTIR	659:662	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	3	28	theme	solution	476:483	arg1	pH					485:486	solution pH	476:486	solution pH	476:486	Batch removal of chromium (Cr) was optimized with respect to solution pH, batch stirring time, sorbent dose, initial chromium concentration and temperature.					
36470349	0	29	theme	porous	19:24	arg1	nanocomposite					50:62	porous magnetic Chitosan/Fe3O4 nanocomposite	19:62	porous magnetic Chitosan/Fe3O4 nanocomposite	19:62	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	1	30	theme	co-precipitation	301:316	arg1	method					335:340	co-precipitation and hydrothermal method	301:340	co-precipitation and hydrothermal method	301:340	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	12	31	theme	@	1722:1722	arg1	adsorbent					1764:1772	adsorbent	1764:1772	adsorbent	1764:1772	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	12	31	theme	@	1722:1722	arg1	nanocomposite					1739:1751	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	1702:1751	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	1702:1751	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	5	32	dep	Fourier	631:637	arg1	transform					639:647	transform	639:647	transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite	639:746	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	11	33	with	modification	1450:1461	arg1	chitosan					1486:1493	chitosan	1486:1493	chitosan	1486:1493	Surface modification of Azolla pinnata with chitosan and iron oxide improved the ability of Azolla in the adsorption of chromium from aqueous media.					
36470349	11	33	with	modification	1450:1461	arg1	oxide					1504:1508	iron oxide	1499:1508	iron oxide	1499:1508	Surface modification of Azolla pinnata with chitosan and iron oxide improved the ability of Azolla in the adsorption of chromium from aqueous media.					
36470349	0	34	theme	Chitosan/Fe3O4	35:48	arg1	nanocomposite					50:62	porous magnetic Chitosan/Fe3O4 nanocomposite	19:62	porous magnetic Chitosan/Fe3O4 nanocomposite	19:62	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	1	35	theme	hydrothermal	322:333	arg1	method					335:340	co-precipitation and hydrothermal method	301:340	co-precipitation and hydrothermal method	301:340	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	5	36	theme	characteristic	774:787	arg1	groups					800:805	characteristic functional groups	774:805	characteristic functional groups	774:805	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	0	37	dep	using	64:68	arg1	characterization					139:154	surface characterization	131:154	surface characterization	131:154	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	0	37	dep	using	64:68	arg1	kinetics					160:167	kinetics	160:167	kinetics	160:167	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	0	37	dep	using	64:68	arg1	effects					122:128	Parametric effects	111:128	Parametric effects	111:128	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	3	38	theme	stirring	495:502	arg1	time					504:507	batch stirring time	489:507	batch stirring time	489:507	Batch removal of chromium (Cr) was optimized with respect to solution pH, batch stirring time, sorbent dose, initial chromium concentration and temperature.					
36470349	5	39	theme	groups	800:805	arg1	presence					762:769	the presence	758:769	the presence of characteristic functional groups and porous structure of synthesized nanocomposite	758:855	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	12	40	dep	separation	1682:1691	arg1	promotes					1693:1700	promotes	1693:1700	promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent	1693:1772	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	1	41	theme	Chitosan-iron-oxide	225:243	arg1	nanocomposite					203:215	a novel porous nanocomposite	188:215	a novel porous nanocomposite	188:215	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	1	41	theme	Chitosan-iron-oxide	225:243	arg1	nanocomposite					262:274	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	225:274	namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite	218:274	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	9	42	theme	Reusability	1174:1184	arg1	studies					1186:1192	Reusability studies	1174:1192	Reusability studies	1174:1192	Reusability studies have confirmed that removal efficiency attained was 85% after completion of five adsorption-desorption cycles.					
36470349	1	43	theme	Azolla	247:252	arg1	nanocomposite					203:215	a novel porous nanocomposite	188:215	a novel porous nanocomposite	188:215	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	1	43	theme	Azolla	247:252	arg1	nanocomposite					262:274	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	225:274	namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite	218:274	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	5	44	theme	functional	789:798	arg1	groups					800:805	characteristic functional groups	774:805	characteristic functional groups	774:805	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	5	45	theme	infrared	649:656	arg1	spectroscopy					665:676	infrared (FTIR) spectroscopy	649:676	infrared (FTIR) spectroscopy	649:676	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	2	46	theme	adsorption	378:387	arg1	process					389:395	adsorption process	378:395	adsorption process	378:395	The effect of process parameter on adsorption process was investigated.					
36470349	4	47	theme	removal	584:590	arg1	efficiency					592:601	The maximum removal efficiency	572:601	The maximum removal efficiency	572:601	The maximum removal efficiency was found to be 98.58%.					
36470349	4	47	theme	removal	584:590	arg1	%					624:624	98.58%	619:624	98.58%	619:624	The maximum removal efficiency was found to be 98.58%.					
36470349	11	48	theme	Azolla	1466:1471	arg1	pinnata					1473:1479	Azolla pinnata	1466:1479	Azolla pinnata	1466:1479	Surface modification of Azolla pinnata with chitosan and iron oxide improved the ability of Azolla in the adsorption of chromium from aqueous media.					
36470349	2	49	theme	parameter	365:373	arg1	effect					347:352	The effect	343:352	The effect of process parameter on adsorption process	343:395	The effect of process parameter on adsorption process was investigated.					
36470349	3	50	theme	initial	524:530	arg1	concentration					541:553	initial chromium concentration	524:553	initial chromium concentration	524:553	Batch removal of chromium (Cr) was optimized with respect to solution pH, batch stirring time, sorbent dose, initial chromium concentration and temperature.					
36470349	6	51	theme	Langmuir	895:902	arg1	adsorption					904:913	Langmuir adsorption	895:913	Langmuir adsorption isotherm (R2 = 0.996) confirming mono layer sorption	895:966	The adsorption data fitted well with Langmuir adsorption isotherm (R2 = 0.996) confirming mono layer sorption and the maximum uptake was found to be 294.12 mg/g.					
36470349	0	52	theme	surface	131:137	arg1	characterization					139:154	surface characterization	131:154	surface characterization	131:154	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	0	52	theme	surface	131:137	arg1	effects					122:128	Parametric effects	111:128	Parametric effects	111:128	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	5	53	theme	synthesized	831:841	arg1	nanocomposite					843:855	synthesized nanocomposite	831:855	synthesized nanocomposite	831:855	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	12	54	theme	magnetic	1673:1680	arg1	separation					1682:1691	magnetic separation	1673:1691	magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent	1673:1772	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	7	55	theme	second	1062:1067	arg1	R2 = 0.997					1082:1091	R2 = 0.997	1082:1091	R2 = 0.997	1082:1091	The adsorption was found to follow pseudo second order model (R2 = 0.997).					
36470349	7	55	theme	second	1062:1067	arg1	model					1075:1079	pseudo second order model	1055:1079	pseudo second order model (R2 = 0.997)	1055:1092	The adsorption was found to follow pseudo second order model (R2 = 0.997).					
36470349	12	56	theme	adsorption	1642:1651	arg1	features					1653:1660	adsorption features	1642:1660	adsorption features	1642:1660	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	5	57	theme	microscope	700:709	arg1	analysis					717:724	scanning electron microscope (SEM) analysis	682:724	scanning electron microscope (SEM) analysis of the nano composite	682:746	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	5	58	theme	scanning	682:689	arg1	SEM					712:714	SEM	712:714	SEM	712:714	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	5	58	theme	scanning	682:689	arg1	microscope					700:709	scanning electron microscope	682:709	scanning electron microscope (SEM) analysis of the nano composite	682:746	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	9	59	theme	cycles	1297:1302	arg1	completion					1256:1265	completion	1256:1265	completion of five adsorption-desorption cycles	1256:1302	Reusability studies have confirmed that removal efficiency attained was 85% after completion of five adsorption-desorption cycles.					
36470349	11	60	mod	modification	1450:1461	arg3	Surface					1442:1448	Surface modification	1442:1461	Surface modification of Azolla pinnata with chitosan and iron oxide	1442:1508	Surface modification of Azolla pinnata with chitosan and iron oxide improved the ability of Azolla in the adsorption of chromium from aqueous media.					
36470349	11	60	mod	modification	1450:1461	arg1	pinnata					1473:1479	Azolla pinnata	1466:1479	Azolla pinnata	1466:1479	Surface modification of Azolla pinnata with chitosan and iron oxide improved the ability of Azolla in the adsorption of chromium from aqueous media.					
36470349	5	61	theme	composite	738:746	arg1	spectroscopy					665:676	infrared (FTIR) spectroscopy	649:676	infrared (FTIR) spectroscopy	649:676	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	5	61	theme	composite	738:746	arg1	analysis					717:724	scanning electron microscope (SEM) analysis	682:724	scanning electron microscope (SEM) analysis of the nano composite	682:746	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	1	62	theme	porous	196:201	arg1	nanocomposite					203:215	a novel porous nanocomposite	188:215	a novel porous nanocomposite	188:215	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	1	62	theme	porous	196:201	arg1	nanocomposite					262:274	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	225:274	namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite	218:274	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	12	63	theme	facile	1615:1620	arg1	synthesis					1622:1630	facile synthesis	1615:1630	facile synthesis	1615:1630	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	11	64	theme	chromium	1562:1569	arg1	adsorption					1548:1557	the adsorption	1544:1557	the adsorption of chromium from aqueous media	1544:1588	Surface modification of Azolla pinnata with chitosan and iron oxide improved the ability of Azolla in the adsorption of chromium from aqueous media.					
36470349	6	65	theme	maximum	976:982	arg1	294.12 mg/g					1007:1017	294.12 mg/g	1007:1017	294.12 mg/g	1007:1017	The adsorption data fitted well with Langmuir adsorption isotherm (R2 = 0.996) confirming mono layer sorption and the maximum uptake was found to be 294.12 mg/g.					
36470349	6	65	theme	maximum	976:982	arg1	uptake					984:989	the maximum uptake	972:989	the maximum uptake	972:989	The adsorption data fitted well with Langmuir adsorption isotherm (R2 = 0.996) confirming mono layer sorption and the maximum uptake was found to be 294.12 mg/g.					
36470349	12	66	theme	Azolla	1724:1729	arg1	adsorbent					1764:1772	adsorbent	1764:1772	adsorbent	1764:1772	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	12	66	theme	Azolla	1724:1729	arg1	nanocomposite					1739:1751	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	1702:1751	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	1702:1751	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	0	67	theme	magnetic	26:33	arg1	nanocomposite					50:62	porous magnetic Chitosan/Fe3O4 nanocomposite	19:62	porous magnetic Chitosan/Fe3O4 nanocomposite	19:62	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	4	68	theme	maximum	576:582	arg1	efficiency					592:601	The maximum removal efficiency	572:601	The maximum removal efficiency	572:601	The maximum removal efficiency was found to be 98.58%.					
36470349	4	68	theme	maximum	576:582	arg1	%					624:624	98.58%	619:624	98.58%	619:624	The maximum removal efficiency was found to be 98.58%.					
36470349	10	69	theme	coordination	1345:1356	arg1	attraction					1319:1328	Electrostatic attraction	1305:1328	Electrostatic attraction	1305:1328	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	69	theme	coordination	1345:1356	arg1	exchange					1335:1342	ion exchange	1331:1342	ion exchange	1331:1342	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	69	theme	coordination	1345:1356	arg1	bonding					1358:1364	coordination bonding	1345:1364	coordination bonding	1345:1364	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	69	theme	coordination	1345:1356	arg1	reduction					1370:1378	reduction	1370:1378	reduction	1370:1378	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	69	theme	coordination	1345:1356	arg1	mechanisms					1394:1403	the major mechanisms	1384:1403	the major mechanisms responsible for removal of chromium	1384:1439	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	0	70	theme	nanocomposite	50:62	arg1	Bio-fabrication					0:14	Bio-fabrication	0:14	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite	0:62	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	10	71	theme	ion	1331:1333	arg1	attraction					1319:1328	Electrostatic attraction	1305:1328	Electrostatic attraction	1305:1328	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	71	theme	ion	1331:1333	arg1	exchange					1335:1342	ion exchange	1331:1342	ion exchange	1331:1342	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	71	theme	ion	1331:1333	arg1	bonding					1358:1364	coordination bonding	1345:1364	coordination bonding	1345:1364	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	71	theme	ion	1331:1333	arg1	reduction					1370:1378	reduction	1370:1378	reduction	1370:1378	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	71	theme	ion	1331:1333	arg1	mechanisms					1394:1403	the major mechanisms	1384:1403	the major mechanisms responsible for removal of chromium	1384:1439	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	8	72	theme	Thermodynamic	1095:1107	arg1	studies					1109:1115	Thermodynamic studies	1095:1115	Thermodynamic studies	1095:1115	Thermodynamic studies revealed that adsorption is endothermic and spontaneous.					
36470349	12	73	theme	Chitosan-iron-oxide	1702:1720	arg1	adsorbent					1764:1772	adsorbent	1764:1772	adsorbent	1764:1772	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	12	73	theme	Chitosan-iron-oxide	1702:1720	arg1	nanocomposite					1739:1751	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	1702:1751	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	1702:1751	The combined effects of facile synthesis, improved adsorption features and easier magnetic separation promotes Chitosan-iron-oxide @ Azolla pinnata nanocomposite as a novel adsorbent.					
36470349	10	74	theme	major	1388:1392	arg1	attraction					1319:1328	Electrostatic attraction	1305:1328	Electrostatic attraction	1305:1328	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	74	theme	major	1388:1392	arg1	exchange					1335:1342	ion exchange	1331:1342	ion exchange	1331:1342	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	74	theme	major	1388:1392	arg1	bonding					1358:1364	coordination bonding	1345:1364	coordination bonding	1345:1364	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	74	theme	major	1388:1392	arg1	reduction					1370:1378	reduction	1370:1378	reduction	1370:1378	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	74	theme	major	1388:1392	arg1	mechanisms					1394:1403	the major mechanisms	1384:1403	the major mechanisms responsible for removal of chromium	1384:1439	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	5	75	theme	nano	733:736	arg1	composite					738:746	the nano composite	729:746	the nano composite	729:746	The Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM) analysis of the nano composite confirmed the presence of characteristic functional groups and porous structure of synthesized nanocomposite.					
36470349	0	76	theme	Azolla	70:75	arg1	pinnata					77:83	Azolla pinnata	70:83	Azolla pinnata for removal of chromium	70:107	Bio-fabrication of porous magnetic Chitosan/Fe3O4 nanocomposite using Azolla pinnata for removal of chromium - Parametric effects, surface characterization and kinetics.					
36470349	6	77	theme	adsorption	862:871	arg1	data					873:876	The adsorption data	858:876	The adsorption data	858:876	The adsorption data fitted well with Langmuir adsorption isotherm (R2 = 0.996) confirming mono layer sorption and the maximum uptake was found to be 294.12 mg/g.					
36470349	3	78	theme	chromium	432:439	arg1	removal					421:427	Batch removal	415:427	Batch removal of chromium (Cr)	415:444	Batch removal of chromium (Cr) was optimized with respect to solution pH, batch stirring time, sorbent dose, initial chromium concentration and temperature.					
36470349	2	79	theme	process	357:363	arg1	parameter					365:373	process parameter	357:373	process parameter	357:373	The effect of process parameter on adsorption process was investigated.					
36470349	3	80	theme	batch	489:493	arg1	time					504:507	batch stirring time	489:507	batch stirring time	489:507	Batch removal of chromium (Cr) was optimized with respect to solution pH, batch stirring time, sorbent dose, initial chromium concentration and temperature.					
36470349	1	81	theme	@	245:245	arg1	nanocomposite					203:215	a novel porous nanocomposite	188:215	a novel porous nanocomposite	188:215	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	1	81	theme	@	245:245	arg1	nanocomposite					262:274	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	225:274	namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite	218:274	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	10	82	theme	chromium	1432:1439	arg1	removal					1421:1427	removal	1421:1427	removal of chromium	1421:1439	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	11	83	theme	Surface	1442:1448	arg1	modification					1450:1461	Surface modification	1442:1461	Surface modification of Azolla pinnata with chitosan and iron oxide	1442:1508	Surface modification of Azolla pinnata with chitosan and iron oxide improved the ability of Azolla in the adsorption of chromium from aqueous media.					
36470349	10	84	theme	responsible	1405:1415	arg1	attraction					1319:1328	Electrostatic attraction	1305:1328	Electrostatic attraction	1305:1328	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	84	theme	responsible	1405:1415	arg1	exchange					1335:1342	ion exchange	1331:1342	ion exchange	1331:1342	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	84	theme	responsible	1405:1415	arg1	bonding					1358:1364	coordination bonding	1345:1364	coordination bonding	1345:1364	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	84	theme	responsible	1405:1415	arg1	reduction					1370:1378	reduction	1370:1378	reduction	1370:1378	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	10	84	theme	responsible	1405:1415	arg1	mechanisms					1394:1403	the major mechanisms	1384:1403	the major mechanisms responsible for removal of chromium	1384:1439	Electrostatic attraction, ion exchange, coordination bonding and reduction are the major mechanisms responsible for removal of chromium.					
36470349	1	85	theme	pinnata	254:260	arg1	nanocomposite					203:215	a novel porous nanocomposite	188:215	a novel porous nanocomposite	188:215	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
36470349	1	85	theme	pinnata	254:260	arg1	nanocomposite					262:274	Chitosan-iron-oxide @ Azolla pinnata nanocomposite	225:274	namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite	218:274	In this research, a novel porous nanocomposite, namely Chitosan-iron-oxide @ Azolla pinnata nanocomposite, has been synthesized by co-precipitation and hydrothermal method.					
35926229	1	0	with	patients	250:257	arg1	sepsis					264:269	sepsis	264:269	sepsis	264:269	Hemoperfusion is an important method to remove endotoxins and save the lives of patients with sepsis.					
35926229	10	1	theme	adsorbents	1821:1830	arg1	compatibility					1782:1794	poor blood compatibility	1771:1794	poor blood compatibility	1771:1794	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	10	1	theme	adsorbents	1821:1830	arg1	efficiency					1756:1765	low adsorption efficiency	1741:1765	low adsorption efficiency	1741:1765	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	2	2	theme	poor	388:391	arg1	compatibility					399:411	poor blood compatibility	388:411	poor blood compatibility	388:411	However, the current adsorbents for hemoperfusion have disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so on.					
35926229	7	3	from	ratio	1312:1316	arg1	plasma					1334:1339	plasma	1334:1339	plasma	1334:1339	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	3	4	theme	double-network	512:525	arg1	-poly					554:558	double-network carboxylated chitosan (CCS)-poly	512:558	double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA)	512:600	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	4	theme	double-network	512:525	arg1	chloride					585:592	diallyl dimethylammonium chloride	560:592	diallyl dimethylammonium chloride	560:592	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	4	theme	double-network	512:525	arg1	PDDA					596:599	PDDA	596:599	PDDA	596:599	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	9	5	theme	good	1558:1561	arg1	effect					1574:1579	a good inhibitory effect	1556:1579	a good inhibitory effect	1556:1579	Interestingly, the ET-CCSPDs had a good inhibitory effect on the cytokines produced by endotoxin-mediated septic blood.					
35926229	7	6	theme	=	1366:1366	arg1	level					1397:1401	critical illness level	1380:1401	critical illness level	1380:1401	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	7	6	theme	=	1366:1366	arg1	EU/mL					1373:1377	C0 = 5.50 EU/mL	1363:1377	C0 = 5.50 EU/mL	1363:1377	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	2	7	theme	current	285:291	arg1	adsorbents					293:302	the current adsorbents	281:302	the current adsorbents for hemoperfusion	281:320	However, the current adsorbents for hemoperfusion have disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so on.					
35926229	7	8	theme	illness	1389:1395	arg1	level					1397:1401	critical illness level	1380:1401	critical illness level	1380:1401	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	7	8	theme	illness	1389:1395	arg1	EU/mL					1373:1377	C0 = 5.50 EU/mL	1363:1377	C0 = 5.50 EU/mL	1363:1377	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	4	9	theme	adsorption	859:868	arg1	efficacy					870:877	enhanced adsorption efficacy	850:877	enhanced adsorption efficacy	850:877	CCS was introduced to balance the strong positive charges of PDDA to improve hemocompatibility, and emulsion templates endowed the adsorbent with an ultraporous structure for enhanced adsorption efficacy.					
35926229	6	10	theme	partial	985:991	arg1	APTT					1014:1017	APTT	1014:1017	APTT	1014:1017	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	6	10	theme	partial	985:991	arg1	time					1008:1011	the activated partial thromboplastin time	971:1011	the activated partial thromboplastin time (APTT)	971:1018	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	6	10	theme	partial	985:991	arg1	times					1041:1045	prolonged to 8.5 times	1024:1045	prolonged to 8.5 times	1024:1045	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	10	11	theme	new	1865:1867	arg1	route					1869:1873	a new route	1863:1873	a new route to fabricate absorbents for blood purification	1863:1920	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	3	12	theme	dimethylammonium	568:583	arg1	-poly					554:558	double-network carboxylated chitosan (CCS)-poly	512:558	double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA)	512:600	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	12	theme	dimethylammonium	568:583	arg1	chloride					585:592	diallyl dimethylammonium chloride	560:592	diallyl dimethylammonium chloride	560:592	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	10	13	theme	blood	1903:1907	arg1	purification					1909:1920	blood purification	1903:1920	blood purification	1903:1920	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	3	14	theme	hydrogel	602:609	arg1	ET-CCSPD					620:627	ET-CCSPD	620:627	ET-CCSPD	620:627	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	14	theme	hydrogel	602:609	arg1	spheres					611:617	hydrogel spheres	602:617	hydrogel spheres (ET-CCSPD)	602:628	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	9	15	theme	endotoxin-mediated	1610:1627	arg1	blood					1636:1640	endotoxin-mediated septic blood	1610:1640	endotoxin-mediated septic blood	1610:1640	Interestingly, the ET-CCSPDs had a good inhibitory effect on the cytokines produced by endotoxin-mediated septic blood.					
35926229	4	16	theme	positive	716:723	arg1	charges					725:731	the strong positive charges	705:731	the strong positive charges of PDDA	705:739	CCS was introduced to balance the strong positive charges of PDDA to improve hemocompatibility, and emulsion templates endowed the adsorbent with an ultraporous structure for enhanced adsorption efficacy.					
35926229	8	17	theme	adsorption	1408:1417	arg1	cartridge					1419:1427	An adsorption cartridge	1405:1427	An adsorption cartridge filled with the ET-CCSPDs	1405:1453	An adsorption cartridge filled with the ET-CCSPDs could remove 84.7% of endotoxin within 1 h (C0 = 100 EU/mL in PBS).					
35926229	7	18	dep	%	1360:1360	arg1	level					1397:1401	critical illness level	1380:1401	critical illness level	1380:1401	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	7	18	dep	%	1360:1360	arg1	EU/mL					1373:1377	C0 = 5.50 EU/mL	1363:1377	C0 = 5.50 EU/mL	1363:1377	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	1	19	theme	important	190:198	arg1	method					200:205	an important method	187:205	an important method to remove endotoxins and save the lives of patients with sepsis	187:269	Hemoperfusion is an important method to remove endotoxins and save the lives of patients with sepsis.					
35926229	1	19	theme	important	190:198	arg1	Hemoperfusion					170:182	Hemoperfusion	170:182	Hemoperfusion	170:182	Hemoperfusion is an important method to remove endotoxins and save the lives of patients with sepsis.					
35926229	0	20	theme	Rapid	115:119	arg1	Profiles					139:146	Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles	81:146	Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment	81:167	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.					
35926229	3	21	theme	emulsion	453:460	arg1	method					478:483	a novel emulsion templating (ET) method	445:483	a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD)	445:628	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	0	22	theme	Removal	131:137	arg1	Profiles					139:146	Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles	81:146	Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment	81:167	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.					
35926229	0	23	theme	Antibacterial	96:108	arg1	Profiles					139:146	Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles	81:146	Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment	81:167	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.					
35926229	2	24	theme	so	418:419	arg1	disadvantages					327:339	disadvantages	327:339	disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so	327:419	However, the current adsorbents for hemoperfusion have disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so on.					
35926229	0	25	theme	Ultraporous	0:10	arg1	Spheres					68:74	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres	0:74	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.	0:168	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.					
35926229	7	26	theme	static	1297:1302	arg1	high					1348:1351	high	1348:1351	high	1348:1351	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	7	26	theme	static	1297:1302	arg1	ratio					1312:1316	the static removal ratio	1293:1316	the static removal ratio of endotoxin in plasma	1293:1339	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	0	27	theme	Chitosan	40:47	arg1	Spheres					68:74	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres	0:74	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.	0:168	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.					
35926229	3	28	theme	negative	644:651	arg1	charges					666:672	both negative and positive charges	639:672	both negative and positive charges	639:672	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	0	29	theme	Hydrogel	59:66	arg1	Spheres					68:74	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres	0:74	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.	0:168	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.					
35926229	2	30	contain	have	322:325	arg2	disadvantages					327:339	disadvantages	327:339	disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so	327:419	However, the current adsorbents for hemoperfusion have disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so on.					
35926229	2	30	contain	have	322:325	arg1	adsorbents					293:302	the current adsorbents	281:302	the current adsorbents for hemoperfusion	281:320	However, the current adsorbents for hemoperfusion have disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so on.					
35926229	10	31	theme	adsorption	1745:1754	arg1	efficiency					1756:1765	low adsorption efficiency	1741:1765	low adsorption efficiency	1741:1765	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	4	32	theme	ultraporous	824:834	arg1	structure					836:844	an ultraporous structure	821:844	an ultraporous structure for enhanced adsorption efficacy	821:877	CCS was introduced to balance the strong positive charges of PDDA to improve hemocompatibility, and emulsion templates endowed the adsorbent with an ultraporous structure for enhanced adsorption efficacy.					
35926229	7	33	theme	excellent	1144:1152	arg1	performance					1165:1175	excellent scavenging performance	1144:1175	excellent scavenging performance	1144:1175	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	2	34	theme	adsorption	367:376	arg1	capacity					378:385	insufficient endotoxin adsorption capacity	344:385	insufficient endotoxin adsorption capacity	344:385	However, the current adsorbents for hemoperfusion have disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so on.					
35926229	3	35	theme	positive	657:664	arg1	charges					666:672	both negative and positive charges	639:672	both negative and positive charges	639:672	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	6	36	from	injection	1086:1094	arg1	patients					1116:1123	patients	1116:1123	patients	1116:1123	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	3	37	theme	chitosan	540:547	arg1	-poly					554:558	double-network carboxylated chitosan (CCS)-poly	512:558	double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA)	512:600	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	37	theme	chitosan	540:547	arg1	chloride					585:592	diallyl dimethylammonium chloride	560:592	diallyl dimethylammonium chloride	560:592	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	37	theme	chitosan	540:547	arg1	PDDA					596:599	PDDA	596:599	PDDA	596:599	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	38	dep	method	478:483	arg1	ET					474:475	ET	474:475	ET	474:475	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	6	39	theme	anticoagulant	1099:1111	arg1	injection					1086:1094	the injection	1082:1094	the injection of anticoagulant in patients	1082:1123	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	2	40	theme	blood	393:397	arg1	compatibility					399:411	poor blood compatibility	388:411	poor blood compatibility	388:411	However, the current adsorbents for hemoperfusion have disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so on.					
35926229	10	41	theme	endotoxin	1811:1819	arg1	adsorbents					1821:1830	traditional endotoxin adsorbents	1799:1830	traditional endotoxin adsorbents	1799:1830	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	7	42	contain	had	1140:1142	arg2	performance					1165:1175	excellent scavenging performance	1144:1175	excellent scavenging performance	1144:1175	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	7	42	contain	had	1140:1142	arg1	ET-CCSPDs					1130:1138	The ET-CCSPDs	1126:1138	The ET-CCSPDs	1126:1138	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	10	43	theme	ET	1661:1662	arg1	method					1664:1669	the ET method	1657:1669	the ET method to prepare ultraporous and double-network adsorbents	1657:1722	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	10	44	theme	ultraporous	1682:1692	arg1	adsorbents					1713:1722	ultraporous and double-network adsorbents	1682:1722	ultraporous and double-network adsorbents	1682:1722	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	8	45	from	=	1502:1502	arg1	PBS					1517:1519	PBS	1517:1519	PBS	1517:1519	An adsorption cartridge filled with the ET-CCSPDs could remove 84.7% of endotoxin within 1 h (C0 = 100 EU/mL in PBS).					
35926229	3	46	theme	CCS	550:552	arg1	-poly					554:558	double-network carboxylated chitosan (CCS)-poly	512:558	double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA)	512:600	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	46	theme	CCS	550:552	arg1	chloride					585:592	diallyl dimethylammonium chloride	560:592	diallyl dimethylammonium chloride	560:592	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	46	theme	CCS	550:552	arg1	PDDA					596:599	PDDA	596:599	PDDA	596:599	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	7	47	theme	critical	1380:1387	arg1	level					1397:1401	critical illness level	1380:1401	critical illness level	1380:1401	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	7	47	theme	critical	1380:1387	arg1	EU/mL					1373:1377	C0 = 5.50 EU/mL	1363:1377	C0 = 5.50 EU/mL	1363:1377	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	9	48	theme	inhibitory	1563:1572	arg1	effect					1574:1579	a good inhibitory effect	1556:1579	a good inhibitory effect	1556:1579	Interestingly, the ET-CCSPDs had a good inhibitory effect on the cytokines produced by endotoxin-mediated septic blood.					
35926229	7	49	theme	5.50	1368:1371	arg1	level					1397:1401	critical illness level	1380:1401	critical illness level	1380:1401	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	7	49	theme	5.50	1368:1371	arg1	EU/mL					1373:1377	C0 = 5.50 EU/mL	1363:1377	C0 = 5.50 EU/mL	1363:1377	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	6	50	from	times	1041:1045	arg1	addition					961:968	addition	961:968	addition	961:968	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	2	51	theme	insufficient	344:355	arg1	capacity					378:385	insufficient endotoxin adsorption capacity	344:385	insufficient endotoxin adsorption capacity	344:385	However, the current adsorbents for hemoperfusion have disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so on.					
35926229	4	52	dep	templates	784:792	arg1	endowed					794:800	endowed	794:800	endowed	794:800	CCS was introduced to balance the strong positive charges of PDDA to improve hemocompatibility, and emulsion templates endowed the adsorbent with an ultraporous structure for enhanced adsorption efficacy.					
35926229	6	53	theme	thromboplastin	993:1006	arg1	APTT					1014:1017	APTT	1014:1017	APTT	1014:1017	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	6	53	theme	thromboplastin	993:1006	arg1	time					1008:1011	the activated partial thromboplastin time	971:1011	the activated partial thromboplastin time (APTT)	971:1018	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	6	53	theme	thromboplastin	993:1006	arg1	times					1041:1045	prolonged to 8.5 times	1024:1045	prolonged to 8.5 times	1024:1045	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	4	54	theme	enhanced	850:857	arg1	efficacy					870:877	enhanced adsorption efficacy	850:877	enhanced adsorption efficacy	850:877	CCS was introduced to balance the strong positive charges of PDDA to improve hemocompatibility, and emulsion templates endowed the adsorbent with an ultraporous structure for enhanced adsorption efficacy.					
35926229	7	55	theme	C0	1363:1364	arg1	level					1397:1401	critical illness level	1380:1401	critical illness level	1380:1401	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	7	55	theme	C0	1363:1364	arg1	EU/mL					1373:1377	C0 = 5.50 EU/mL	1363:1377	C0 = 5.50 EU/mL	1363:1377	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	8	56	from	PBS	1517:1519	arg1	=					1502:1502	=	1502:1502	=	1502:1502	An adsorption cartridge filled with the ET-CCSPDs could remove 84.7% of endotoxin within 1 h (C0 = 100 EU/mL in PBS).					
35926229	3	57	theme	diallyl	560:566	arg1	-poly					554:558	double-network carboxylated chitosan (CCS)-poly	512:558	double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA)	512:600	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	57	theme	diallyl	560:566	arg1	chloride					585:592	diallyl dimethylammonium chloride	560:592	diallyl dimethylammonium chloride	560:592	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	6	58	theme	activated	975:983	arg1	APTT					1014:1017	APTT	1014:1017	APTT	1014:1017	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	6	58	theme	activated	975:983	arg1	time					1008:1011	the activated partial thromboplastin time	971:1011	the activated partial thromboplastin time (APTT)	971:1018	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	6	58	theme	activated	975:983	arg1	times					1041:1045	prolonged to 8.5 times	1024:1045	prolonged to 8.5 times	1024:1045	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	7	59	theme	removal	1214:1220	arg1	ratios					1222:1227	removal ratios	1214:1227	removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively	1214:1286	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	8	60	dep	h	1496:1496	arg1	=					1502:1502	=	1502:1502	=	1502:1502	An adsorption cartridge filled with the ET-CCSPDs could remove 84.7% of endotoxin within 1 h (C0 = 100 EU/mL in PBS).					
35926229	10	61	theme	double-network	1698:1711	arg1	adsorbents					1713:1722	ultraporous and double-network adsorbents	1682:1722	ultraporous and double-network adsorbents	1682:1722	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	0	62	theme	Sepsis	152:157	arg1	Treatment					159:167	Sepsis Treatment	152:167	Sepsis Treatment	152:167	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.					
35926229	9	63	theme	septic	1629:1634	arg1	blood					1636:1640	endotoxin-mediated septic blood	1610:1640	endotoxin-mediated septic blood	1610:1640	Interestingly, the ET-CCSPDs had a good inhibitory effect on the cytokines produced by endotoxin-mediated septic blood.					
35926229	10	64	theme	low	1741:1743	arg1	efficiency					1756:1765	low adsorption efficiency	1741:1765	low adsorption efficiency	1741:1765	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	5	65	theme	complement	936:945	arg1	responses					947:955	complement responses	936:955	complement responses	936:955	The ET-CCSPDs neither damaged blood cells nor activated complement responses.					
35926229	4	66	theme	strong	709:714	arg1	charges					725:731	the strong positive charges	705:731	the strong positive charges of PDDA	705:739	CCS was introduced to balance the strong positive charges of PDDA to improve hemocompatibility, and emulsion templates endowed the adsorbent with an ultraporous structure for enhanced adsorption efficacy.					
35926229	7	67	theme	%	1236:1236	arg1	ratios					1222:1227	removal ratios	1214:1227	removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively	1214:1286	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	7	68	theme	endotoxin	1321:1329	arg1	high					1348:1351	high	1348:1351	high	1348:1351	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	7	68	theme	endotoxin	1321:1329	arg1	ratio					1312:1316	the static removal ratio	1293:1316	the static removal ratio of endotoxin in plasma	1293:1339	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	5	69	theme	blood	910:914	arg1	cells					916:920	blood cells	910:920	blood cells	910:920	The ET-CCSPDs neither damaged blood cells nor activated complement responses.					
35926229	4	70	with	endowed	794:800	arg1	structure					836:844	an ultraporous structure	821:844	an ultraporous structure for enhanced adsorption efficacy	821:877	CCS was introduced to balance the strong positive charges of PDDA to improve hemocompatibility, and emulsion templates endowed the adsorbent with an ultraporous structure for enhanced adsorption efficacy.					
35926229	0	71	theme	Endotoxin	121:129	arg1	Profiles					139:146	Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles	81:146	Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment	81:167	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.					
35926229	6	72	dep	times	1041:1045	arg1	beneficial					1058:1067	beneficial	1058:1067	beneficial	1058:1067	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	3	73	dep	ultraporous	496:506	arg1	ET-CCSPD					620:627	ET-CCSPD	620:627	ET-CCSPD	620:627	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	73	dep	ultraporous	496:506	arg1	spheres					611:617	hydrogel spheres	602:617	hydrogel spheres (ET-CCSPD)	602:628	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	7	74	theme	removal	1304:1310	arg1	high					1348:1351	high	1348:1351	high	1348:1351	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	7	74	theme	removal	1304:1310	arg1	ratio					1312:1316	the static removal ratio	1293:1316	the static removal ratio of endotoxin in plasma	1293:1339	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	6	75	dep	8.5	1037:1039	arg1	to					1034:1035	to	1034:1035	to	1034:1035	In addition, the activated partial thromboplastin time (APTT) was prolonged to 8.5 times, which was beneficial for reducing the injection of anticoagulant in patients.					
35926229	3	76	theme	templating	462:471	arg1	method					478:483	a novel emulsion templating (ET) method	445:483	a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD)	445:628	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	0	77	theme	Polyquaternium-Carboxylated	12:38	arg1	Spheres					68:74	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres	0:74	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.	0:168	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.					
35926229	8	78	theme	endotoxin	1477:1485	arg1	endotoxin					1477:1485	endotoxin	1477:1485	endotoxin	1477:1485	An adsorption cartridge filled with the ET-CCSPDs could remove 84.7% of endotoxin within 1 h (C0 = 100 EU/mL in PBS).					
35926229	8	78	theme	endotoxin	1477:1485	arg1	%					1472:1472	84.7%	1468:1472	84.7% of endotoxin	1468:1485	An adsorption cartridge filled with the ET-CCSPDs could remove 84.7% of endotoxin within 1 h (C0 = 100 EU/mL in PBS).					
35926229	10	79	theme	blood	1776:1780	arg1	compatibility					1782:1794	poor blood compatibility	1771:1794	poor blood compatibility	1771:1794	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	3	80	theme	novel	447:451	arg1	method					478:483	a novel emulsion templating (ET) method	445:483	a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD)	445:628	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	0	81	theme	Composite	49:57	arg1	Spheres					68:74	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres	0:74	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.	0:168	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.					
35926229	10	82	theme	poor	1771:1774	arg1	compatibility					1782:1794	poor blood compatibility	1771:1794	poor blood compatibility	1771:1794	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	4	83	theme	PDDA	736:739	arg1	charges					725:731	the strong positive charges	705:731	the strong positive charges of PDDA	705:739	CCS was introduced to balance the strong positive charges of PDDA to improve hemocompatibility, and emulsion templates endowed the adsorbent with an ultraporous structure for enhanced adsorption efficacy.					
35926229	7	84	theme	scavenging	1154:1163	arg1	performance					1165:1175	excellent scavenging performance	1144:1175	excellent scavenging performance	1144:1175	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	10	85	theme	efficiency	1756:1765	arg1	problems					1729:1736	the problems	1725:1736	the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents	1725:1830	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	0	86	theme	Anticoagulant	81:93	arg1	Profiles					139:146	Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles	81:146	Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment	81:167	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.					
35926229	10	87	theme	traditional	1799:1809	arg1	adsorbents					1821:1830	traditional endotoxin adsorbents	1799:1830	traditional endotoxin adsorbents	1799:1830	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	3	88	theme	carboxylated	527:538	arg1	-poly					554:558	double-network carboxylated chitosan (CCS)-poly	512:558	double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA)	512:600	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	88	theme	carboxylated	527:538	arg1	chloride					585:592	diallyl dimethylammonium chloride	560:592	diallyl dimethylammonium chloride	560:592	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	3	88	theme	carboxylated	527:538	arg1	PDDA					596:599	PDDA	596:599	PDDA	596:599	Herein, we proposed a novel emulsion templating (ET) method to prepare ultraporous and double-network carboxylated chitosan (CCS)-poly(diallyl dimethylammonium chloride) (PDDA) hydrogel spheres (ET-CCSPD), bearing both negative and positive charges.					
35926229	2	89	theme	capacity	378:385	arg1	disadvantages					327:339	disadvantages	327:339	disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so	327:419	However, the current adsorbents for hemoperfusion have disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so on.					
35926229	7	90	theme	%	1258:1258	arg1	ratios					1222:1227	removal ratios	1214:1227	removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively	1214:1286	The ET-CCSPDs had excellent scavenging performance against bacteria and endotoxin, with removal ratios of 96.7% for E. coli and 99.8% for S. aureus, respectively, and the static removal ratio of endotoxin in plasma was as high as 99.1% (C0 = 5.50 EU/mL, critical illness level).					
35926229	10	91	theme	compatibility	1782:1794	arg1	problems					1729:1736	the problems	1725:1736	the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents	1725:1830	By developing the ET method to prepare ultraporous and double-network adsorbents, the problems of low adsorption efficiency and poor blood compatibility of traditional endotoxin adsorbents have been solved, thus opening a new route to fabricate absorbents for blood purification.					
35926229	4	92	theme	emulsion	775:782	arg1	templates					784:792	emulsion templates	775:792	emulsion templates endowed the adsorbent with an ultraporous structure for enhanced adsorption efficacy	775:877	CCS was introduced to balance the strong positive charges of PDDA to improve hemocompatibility, and emulsion templates endowed the adsorbent with an ultraporous structure for enhanced adsorption efficacy.					
35926229	2	93	theme	endotoxin	357:365	arg1	capacity					378:385	insufficient endotoxin adsorption capacity	344:385	insufficient endotoxin adsorption capacity	344:385	However, the current adsorbents for hemoperfusion have disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so on.					
35926229	1	94	theme	patients	250:257	arg1	lives					241:245	the lives	237:245	the lives of patients with sepsis	237:269	Hemoperfusion is an important method to remove endotoxins and save the lives of patients with sepsis.					
35926229	9	95	contain	had	1552:1554	arg2	effect					1574:1579	a good inhibitory effect	1556:1579	a good inhibitory effect	1556:1579	Interestingly, the ET-CCSPDs had a good inhibitory effect on the cytokines produced by endotoxin-mediated septic blood.					
35926229	9	95	contain	had	1552:1554	arg1	ET-CCSPDs					1542:1550	the ET-CCSPDs	1538:1550	the ET-CCSPDs	1538:1550	Interestingly, the ET-CCSPDs had a good inhibitory effect on the cytokines produced by endotoxin-mediated septic blood.					
35926229	0	96	with	Spheres	68:74	arg1	Profiles					139:146	Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles	81:146	Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment	81:167	Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment.					
35926229	2	97	theme	compatibility	399:411	arg1	disadvantages					327:339	disadvantages	327:339	disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so	327:419	However, the current adsorbents for hemoperfusion have disadvantages of insufficient endotoxin adsorption capacity, poor blood compatibility, and so on.					
35475171	0	0	theme	Protein	85:91	arg1	RBD					93:95	Spike Protein RBD	79:95	Spike Protein RBD	79:95	Distinct Core Glycan and O-Glycoform Utilization of SARS-CoV-2 Omicron Variant Spike Protein RBD Revealed by Top-Down Mass Spectrometry.					
35475171	1	1	theme	neutralizing	332:343	arg1	antibodies					345:354	neutralizing antibodies	332:354	neutralizing antibodies	332:354	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	4	2	theme	mass	941:944	arg1	MS					960:961	MS	960:961	MS	960:961	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	4	2	theme	mass	941:944	arg1	spectrometry					946:957	high-resolution top-down mass spectrometry	916:957	high-resolution top-down mass spectrometry (MS)	916:962	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	3	3	theme	principal	509:517	arg1	target					519:524	the principal target	505:524	the principal target for neutralizing antibodies and therapeutics	505:569	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	1	4	theme	S	239:239	arg1	S-RBD					266:270	S-RBD	266:270	S-RBD	266:270	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	1	4	theme	S	239:239	arg1	domain					258:263	the S receptor-binding domain	235:263	the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies	235:354	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	0	5	theme	Spike	79:83	arg1	RBD					93:95	Spike Protein RBD	79:95	Spike Protein RBD	79:95	Distinct Core Glycan and O-Glycoform Utilization of SARS-CoV-2 Omicron Variant Spike Protein RBD Revealed by Top-Down Mass Spectrometry.					
35475171	1	6	theme	antibodies	345:354	arg1	transmissibility					300:315	transmissibility	300:315	transmissibility	300:315	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	1	6	theme	antibodies	345:354	arg1	evasion					321:327	evasion	321:327	evasion	321:327	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	1	7	theme	B.1.1.529	161:169	arg1	variant					172:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	1	8	theme	receptor-binding	241:256	arg1	S-RBD					266:270	S-RBD	266:270	S-RBD	266:270	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	1	8	theme	receptor-binding	241:256	arg1	domain					258:263	the S receptor-binding domain	235:263	the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies	235:354	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	7	9	theme	O-glycoforms	1255:1266	arg1	utilization					1278:1288	their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1272:1450	their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1272:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	7	9	theme	O-glycoforms	1255:1266	arg1	detail					1237:1242	high resolution detail	1221:1242	high resolution detail of Omicron O-glycoforms	1221:1266	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	2	10	from	protection	450:459	arg1	escape					424:429	viral escape	418:429	viral escape from immunological protection	418:459	But exactly how the mutations in the Omicron variant enhance viral escape from immunological protection remains to be understood.					
35475171	5	11	theme	Omicron	1008:1014	arg1	variant					1016:1022	the Omicron variant	1004:1022	the Omicron variant	1004:1022	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.					
35475171	7	12	theme	Omicron	1360:1366	arg1	variant					1368:1374	the Omicron variant	1356:1374	the Omicron variant which could shed light on how this variant escapes immunological protection	1356:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	6	13	dep	Core	1081:1084	arg1	structures					1108:1117	O-glycan structures	1099:1117	O-glycan structures	1099:1117	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.					
35475171	6	13	dep	Core	1081:1084	arg1	the					1077:1079	the	1077:1079	the	1077:1079	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.					
35475171	3	14	theme	vaccines	723:730	arg1	design					713:718	rational design	704:718	rational design of vaccines and therapeutics	704:747	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	7	15	theme	direct	1301:1306	arg1	evidence					1318:1325	direct molecular evidence	1301:1325	direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1301:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	0	16	theme	Top-Down	109:116	arg1	Spectrometry					123:134	Top-Down Mass Spectrometry	109:134	Top-Down Mass Spectrometry	109:134	Distinct Core Glycan and O-Glycoform Utilization of SARS-CoV-2 Omicron Variant Spike Protein RBD Revealed by Top-Down Mass Spectrometry.					
35475171	7	17	theme	molecular	1308:1316	arg1	evidence					1318:1325	direct molecular evidence	1301:1325	direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1301:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	3	18	dep	remains	497:503	arg1	inform					697:702	inform	697:702	can inform rational design of vaccines and therapeutics	693:747	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	2	19	theme	Omicron	394:400	arg1	variant					402:408	the Omicron variant	390:408	the Omicron variant	390:408	But exactly how the mutations in the Omicron variant enhance viral escape from immunological protection remains to be understood.					
35475171	5	20	gly	O-glycosite	973:983	arg2	Thr376					986:991	Thr376	986:991	Thr376	986:991	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.					
35475171	5	20	gly	O-glycosite	973:983	arg2	O-glycosite					973:983	A novel O-glycosite	965:983	A novel O-glycosite (Thr376) unique to the Omicron variant	965:1022	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.					
35475171	5	21	theme	unique	994:999	arg1	O-glycosite					973:983	A novel O-glycosite	965:983	A novel O-glycosite (Thr376) unique to the Omicron variant	965:1022	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.					
35475171	5	21	theme	unique	994:999	arg1	Thr376					986:991	Thr376	986:991	Thr376	986:991	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.					
35475171	4	22	theme	S-RBD	829:833	arg1	variant					835:841	the Omicron S-RBD variant	817:841	the Omicron S-RBD variant	817:841	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	0	23	theme	Core	9:12	arg1	Glycan					14:19	Distinct Core Glycan	0:19	Distinct Core Glycan	0:19	Distinct Core Glycan and O-Glycoform Utilization of SARS-CoV-2 Omicron Variant Spike Protein RBD Revealed by Top-Down Mass Spectrometry.					
35475171	7	24	theme	Omicron	1247:1253	arg1	O-glycoforms					1255:1266	Omicron O-glycoforms	1247:1266	Omicron O-glycoforms	1247:1266	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	3	25	theme	structural	581:590	arg1	insights					592:599	new structural insights	577:599	new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes	577:691	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	0	26	theme	Distinct	0:7	arg1	Glycan					14:19	Distinct Core Glycan	0:19	Distinct Core Glycan	0:19	Distinct Core Glycan and O-Glycoform Utilization of SARS-CoV-2 Omicron Variant Spike Protein RBD Revealed by Top-Down Mass Spectrometry.					
35475171	4	27	theme	molecular	769:777	arg1	variations					779:788	molecular variations	769:788	molecular variations	769:788	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	1	28	theme	numerous	190:197	arg1	mutations					209:217	numerous spike (S) mutations	190:217	numerous spike (S) mutations	190:217	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	2	29	theme	immunological	436:448	arg1	protection					450:459	immunological protection	436:459	immunological protection	436:459	But exactly how the mutations in the Omicron variant enhance viral escape from immunological protection remains to be understood.					
35475171	6	30	theme	structural	1153:1162	arg1	heterogeneity					1164:1176	the O-glycoform structural heterogeneity	1137:1176	the O-glycoform structural heterogeneity of the three variants	1137:1198	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.					
35475171	4	31	theme	O-glycoform	794:804	arg1	changes					806:812	O-glycoform changes	794:812	O-glycoform changes	794:812	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	1	32	theme	spike	199:203	arg1	mutations					209:217	numerous spike (S) mutations	190:217	numerous spike (S) mutations	190:217	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	5	33	theme	novel	967:971	arg1	O-glycosite					973:983	A novel O-glycosite	965:983	A novel O-glycosite (Thr376) unique to the Omicron variant	965:1022	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.					
35475171	5	33	theme	novel	967:971	arg1	Thr376					986:991	Thr376	986:991	Thr376	986:991	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.					
35475171	0	34	theme	Mass	118:121	arg1	Spectrometry					123:134	Top-Down Mass Spectrometry	109:134	Top-Down Mass Spectrometry	109:134	Distinct Core Glycan and O-Glycoform Utilization of SARS-CoV-2 Omicron Variant Spike Protein RBD Revealed by Top-Down Mass Spectrometry.					
35475171	3	35	theme	changes	685:691	arg1	characterization					628:643	characterization	628:643	characterization	628:643	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	3	35	theme	changes	685:691	arg1	S-RBD					618:622	Omicron S-RBD	610:622	Omicron S-RBD	610:622	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	3	36	theme	new	577:579	arg1	insights					592:599	new structural insights	577:599	new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes	577:691	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	2	37	theme	viral	418:422	arg1	escape					424:429	viral escape	418:429	viral escape from immunological protection	418:459	But exactly how the mutations in the Omicron variant enhance viral escape from immunological protection remains to be understood.					
35475171	1	38	contain	possesses	180:188	arg2	mutations					209:217	numerous spike (S) mutations	190:217	numerous spike (S) mutations	190:217	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	1	38	contain	possesses	180:188	arg1	variant					172:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	3	39	theme	Omicron	610:616	arg1	S-RBD					618:622	Omicron S-RBD	610:622	Omicron S-RBD	610:622	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	3	40	dep	S-RBD	618:622	arg1	the					606:608	the	606:608	the	606:608	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	4	41	dep	wild-type	858:866	arg1	variants					901:908	variants	901:908	variants	901:908	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	0	42	theme	O-Glycoform	25:35	arg1	Utilization					37:47	O-Glycoform Utilization	25:47	O-Glycoform Utilization of SARS-CoV-2 Omicron Variant	25:77	Distinct Core Glycan and O-Glycoform Utilization of SARS-CoV-2 Omicron Variant Spike Protein RBD Revealed by Top-Down Mass Spectrometry.					
35475171	3	43	theme	post-translational	652:669	arg1	changes					685:691	the post-translational glycosylation changes	648:691	the post-translational glycosylation changes	648:691	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	7	44	theme	proteoform	1330:1339	arg1	alterations					1341:1351	proteoform alterations	1330:1351	proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1330:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	0	45	theme	SARS-CoV-2	52:61	arg1	Variant					71:77	SARS-CoV-2 Omicron Variant	52:77	SARS-CoV-2 Omicron Variant	52:77	Distinct Core Glycan and O-Glycoform Utilization of SARS-CoV-2 Omicron Variant Spike Protein RBD Revealed by Top-Down Mass Spectrometry.					
35475171	7	46	theme	high	1221:1224	arg1	detail					1237:1242	high resolution detail	1221:1242	high resolution detail of Omicron O-glycoforms	1221:1266	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	4	47	theme	top-down	932:939	arg1	MS					960:961	MS	960:961	MS	960:961	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	4	47	theme	top-down	932:939	arg1	spectrometry					946:957	high-resolution top-down mass spectrometry	916:957	high-resolution top-down mass spectrometry (MS)	916:962	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	3	48	theme	glycosylation	671:683	arg1	changes					685:691	the post-translational glycosylation changes	648:691	the post-translational glycosylation changes	648:691	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	2	49	from	mutations	377:385	arg1	variant					402:408	the Omicron variant	390:408	the Omicron variant	390:408	But exactly how the mutations in the Omicron variant enhance viral escape from immunological protection remains to be understood.					
35475171	6	50	theme	O-glycoform	1141:1151	arg1	heterogeneity					1164:1176	the O-glycoform structural heterogeneity	1137:1176	the O-glycoform structural heterogeneity of the three variants	1137:1198	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.					
35475171	4	51	theme	high-resolution	916:930	arg1	MS					960:961	MS	960:961	MS	960:961	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	4	51	theme	high-resolution	916:930	arg1	spectrometry					946:957	high-resolution top-down mass spectrometry	916:957	high-resolution top-down mass spectrometry (MS)	916:962	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	7	52	gly	O-glycoforms	1255:1266	arg1	Omicron					1247:1253	Omicron O-glycoforms	1247:1266	Omicron O-glycoforms	1247:1266	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	7	53	from	alterations	1341:1351	arg1	variant					1368:1374	the Omicron variant	1356:1374	the Omicron variant which could shed light on how this variant escapes immunological protection	1356:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	7	54	from	evidence	1318:1325	arg1	variant					1368:1374	the Omicron variant	1356:1374	the Omicron variant which could shed light on how this variant escapes immunological protection	1356:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	4	55	theme	variant	835:841	arg1	variations					779:788	molecular variations	769:788	molecular variations	769:788	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	4	55	theme	variant	835:841	arg1	changes					806:812	O-glycoform changes	794:812	O-glycoform changes	794:812	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	4	56	dep	variations	779:788	arg1	the					765:767	the	765:767	the	765:767	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	7	57	theme	immunological	1427:1439	arg1	protection					1441:1450	immunological protection	1427:1450	immunological protection	1427:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	4	58	theme	Omicron	821:827	arg1	variant					835:841	the Omicron S-RBD variant	817:841	the Omicron S-RBD variant	817:841	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).					
35475171	0	59	dep	Glycan	14:19	arg1	RBD					93:95	Spike Protein RBD	79:95	Spike Protein RBD	79:95	Distinct Core Glycan and O-Glycoform Utilization of SARS-CoV-2 Omicron Variant Spike Protein RBD Revealed by Top-Down Mass Spectrometry.					
35475171	6	60	theme	O-glycan	1099:1106	arg1	structures					1108:1117	O-glycan structures	1099:1117	O-glycan structures	1099:1117	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.					
35475171	0	61	theme	Variant	71:77	arg1	Glycan					14:19	Distinct Core Glycan	0:19	Distinct Core Glycan	0:19	Distinct Core Glycan and O-Glycoform Utilization of SARS-CoV-2 Omicron Variant Spike Protein RBD Revealed by Top-Down Mass Spectrometry.					
35475171	0	61	theme	Variant	71:77	arg1	Utilization					37:47	O-Glycoform Utilization	25:47	O-Glycoform Utilization of SARS-CoV-2 Omicron Variant	25:77	Distinct Core Glycan and O-Glycoform Utilization of SARS-CoV-2 Omicron Variant Spike Protein RBD Revealed by Top-Down Mass Spectrometry.					
35475171	3	62	theme	rational	704:711	arg1	design					713:718	rational design	704:718	rational design of vaccines and therapeutics	704:747	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	1	63	dep	spike	199:203	arg1	S					206:206	S	206:206	S	206:206	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	7	64	theme	alterations	1341:1351	arg1	evidence					1318:1325	direct molecular evidence	1301:1325	direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1301:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	1	65	theme	SARS-CoV-2	141:150	arg1	variant					172:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	6	66	theme	variants	1191:1198	arg1	heterogeneity					1164:1176	the O-glycoform structural heterogeneity	1137:1176	the O-glycoform structural heterogeneity of the three variants	1137:1198	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.					
35475171	0	67	theme	Omicron	63:69	arg1	Variant					71:77	SARS-CoV-2 Omicron Variant	52:77	SARS-CoV-2 Omicron Variant	52:77	Distinct Core Glycan and O-Glycoform Utilization of SARS-CoV-2 Omicron Variant Spike Protein RBD Revealed by Top-Down Mass Spectrometry.					
35475171	3	68	theme	therapeutics	736:747	arg1	design					713:718	rational design	704:718	rational design of vaccines and therapeutics	704:747	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.					
35475171	6	69	gly	heterogeneity	1164:1176	arg1	variants					1191:1198	the three variants	1181:1198	the three variants	1181:1198	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.					
35475171	7	70	from	variant	1368:1374	arg1	evidence					1318:1325	direct molecular evidence	1301:1325	direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1301:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35475171	1	71	theme	Omicron	152:158	arg1	variant					172:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.					
35475171	7	72	theme	resolution	1226:1235	arg1	detail					1237:1242	high resolution detail	1221:1242	high resolution detail of Omicron O-glycoforms	1221:1266	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.					
35545925	6	0	theme	mDAP	977:980	arg1	deamination					983:993	meso-diaminopimelic acid (mDAP) deamination	951:993	meso-diaminopimelic acid (mDAP) deamination	951:993	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	9	1	theme	unique	1691:1696	arg1	acids					1704:1708	unique amino acids	1691:1708	unique amino acids	1691:1708	We also identified strain-specific changes where LESlike1 increased the addition of unique amino acids to the terminus of the stem peptide and LESB58 increased amidase activity.					
35545925	10	2	theme	strain-to-strain	1941:1956	arg1	differences					1958:1968	inherent strain-to-strain differences	1932:1968	inherent strain-to-strain differences	1932:1968	Overall, this study demonstrates that P. aeruginosa PG composition is primarily influenced by nutrient conditions that mimic the CF lung; however, inherent strain-to-strain differences also exist.					
35545925	6	3	theme	acid	971:974	arg1	deamination					983:993	meso-diaminopimelic acid (mDAP) deamination	951:993	meso-diaminopimelic acid (mDAP) deamination	951:993	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	9	4	theme	acids	1704:1708	arg1	addition					1679:1686	the addition	1675:1686	the addition of unique amino acids to the terminus of the stem peptide	1675:1744	We also identified strain-specific changes where LESlike1 increased the addition of unique amino acids to the terminus of the stem peptide and LESB58 increased amidase activity.					
35545925	6	5	theme	unique	853:858	arg1	muropeptides					860:871	448 unique muropeptides	849:871	448 unique muropeptides	849:871	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	14	6	theme	infections	2609:2618	arg1	virulence					2538:2546	virulence	2538:2546	virulence	2538:2546	Analyzing the PG composition of clinical P. aeruginosa epidemic strains provides insights into the increased virulence and antimicrobial resistance of these difficult-to-eradicate infections.					
35545925	14	6	theme	infections	2609:2618	arg1	resistance					2566:2575	antimicrobial resistance	2552:2575	antimicrobial resistance	2552:2575	Analyzing the PG composition of clinical P. aeruginosa epidemic strains provides insights into the increased virulence and antimicrobial resistance of these difficult-to-eradicate infections.					
35545925	12	7	contain	have	2197:2200	arg2	implications					2202:2213	implications	2202:2213	implications for numerous physiological processes, including virulence and antimicrobial resistance	2202:2300	Changes within the PG structure have implications for numerous physiological processes, including virulence and antimicrobial resistance.					
35545925	12	7	contain	have	2197:2200	arg1	Changes					2165:2171	Changes	2165:2171	Changes within the PG structure	2165:2195	Changes within the PG structure have implications for numerous physiological processes, including virulence and antimicrobial resistance.					
35545925	1	8	theme	increased	218:226	arg1	transmissibility					228:243	increased transmissibility	218:243	increased transmissibility	218:243	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	13	9	from	complexity	2372:2381	arg1	aeruginosa					2417:2426	Pseudomonas aeruginosa	2405:2426	Pseudomonas aeruginosa	2405:2426	The identification of highly unique PG modifications illustrates the complexity of this biopolymer in Pseudomonas aeruginosa.					
35545925	10	10	theme	P.	1823:1824	arg1	composition					1840:1850	P. aeruginosa PG composition	1823:1850	P. aeruginosa PG composition	1823:1850	Overall, this study demonstrates that P. aeruginosa PG composition is primarily influenced by nutrient conditions that mimic the CF lung; however, inherent strain-to-strain differences also exist.					
35545925	6	11	theme	mDAP	1023:1026	arg1	residues					1028:1035	mDAP residues	1023:1035	mDAP residues	1023:1035	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	9	12	theme	stem	1733:1736	arg1	peptide					1738:1744	the stem peptide	1729:1744	the stem peptide	1729:1744	We also identified strain-specific changes where LESlike1 increased the addition of unique amino acids to the terminus of the stem peptide and LESB58 increased amidase activity.					
35545925	8	13	theme	cystic	1302:1307	arg1	CF					1319:1320	CF	1319:1320	CF	1319:1320	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	8	13	theme	cystic	1302:1307	arg1	fibrosis					1309:1316	cystic fibrosis	1302:1316	cystic fibrosis (CF) lung infections	1302:1337	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	1	14	theme	antimicrobial	258:270	arg1	resistance					272:281	enhanced antimicrobial resistance	249:281	enhanced antimicrobial resistance	249:281	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	8	15	theme	strains	1234:1240	arg1	composition					1206:1216	The PG composition	1199:1216	The PG composition of the epidemic strains	1199:1240	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	11	16	theme	enzymatic	2101:2109	arg1	activity					2111:2118	the enzymatic activity	2097:2118	the enzymatic activity that functions on this important biopolymer	2097:2162	IMPORTANCE Using peptidoglycomics to examine the global composition of the peptidoglycan (PG) allows insights into the enzymatic activity that functions on this important biopolymer.					
35545925	2	17	theme	cellular	302:309	arg1	mechanisms					311:320	the cellular mechanisms	298:320	the cellular mechanisms behind this heightened virulence and resistance	298:368	Understanding the cellular mechanisms behind this heightened virulence and resistance is critical.					
35545925	11	18	theme	important	2143:2151	arg1	biopolymer					2153:2162	this important biopolymer	2138:2162	this important biopolymer	2138:2162	IMPORTANCE Using peptidoglycomics to examine the global composition of the peptidoglycan (PG) allows insights into the enzymatic activity that functions on this important biopolymer.					
35545925	8	19	theme	sugars	1485:1490	arg1	related					1535:1541	related	1535:1541	related	1535:1541	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	8	19	theme	sugars	1485:1490	arg1	activity					1515:1522	d-transpeptidase activity	1498:1522	d-transpeptidase activity	1498:1522	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	8	19	theme	sugars	1485:1490	arg1	activity					1423:1430	d-endopeptidase activity	1407:1430	d-endopeptidase activity	1407:1430	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	8	19	theme	sugars	1485:1490	arg1	l					1496:1496	l	1496:1496	l	1496:1496	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	8	19	theme	sugars	1485:1490	arg1	abundance					1456:1464	an increased abundance	1443:1464	an increased abundance of de-N-acetylated sugars	1443:1490	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	5	20	theme	PG	715:716	arg1	composition					718:728	PG composition	715:728	PG composition	715:728	We also examined changes in PG composition when the strains were cultured under nutrient conditions that resembled cystic fibrosis lung infections.					
35545925	4	21	dep	strains	664:670	arg1	PA14					681:684	PA14	681:684	PA14	681:684	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	4	21	dep	strains	664:670	arg1	PAO1					672:675	PAO1	672:675	PAO1	672:675	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	4	21	dep	strains	664:670	arg1	strains					664:670	the common laboratory strains	642:670	the common laboratory strains PAO1 and PA14	642:684	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	10	22	theme	PG	1837:1838	arg1	composition					1840:1850	P. aeruginosa PG composition	1823:1850	P. aeruginosa PG composition	1823:1850	Overall, this study demonstrates that P. aeruginosa PG composition is primarily influenced by nutrient conditions that mimic the CF lung; however, inherent strain-to-strain differences also exist.					
35545925	14	23	theme	PG	2443:2444	arg1	composition					2446:2456	the PG composition	2439:2456	the PG composition of clinical P. aeruginosa epidemic strains	2439:2499	Analyzing the PG composition of clinical P. aeruginosa epidemic strains provides insights into the increased virulence and antimicrobial resistance of these difficult-to-eradicate infections.					
35545925	10	24	theme	nutrient	1879:1886	arg1	conditions					1888:1897	nutrient conditions	1879:1897	nutrient conditions that mimic the CF lung	1879:1920	Overall, this study demonstrates that P. aeruginosa PG composition is primarily influenced by nutrient conditions that mimic the CF lung; however, inherent strain-to-strain differences also exist.					
35545925	13	25	theme	biopolymer	2391:2400	arg1	complexity					2372:2381	the complexity	2368:2381	the complexity of this biopolymer in Pseudomonas aeruginosa	2368:2426	The identification of highly unique PG modifications illustrates the complexity of this biopolymer in Pseudomonas aeruginosa.					
35545925	8	26	theme	d-transpeptidase	1498:1513	arg1	activity					1515:1522	d-transpeptidase activity	1498:1522	d-transpeptidase activity	1498:1522	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	5	27	theme	cystic	802:807	arg1	fibrosis					809:816	cystic fibrosis	802:816	cystic fibrosis lung infections	802:832	We also examined changes in PG composition when the strains were cultured under nutrient conditions that resembled cystic fibrosis lung infections.					
35545925	1	28	theme	virulent	180:187	arg1	strains					135:141	Epidemic strains	126:141	Epidemic strains of Pseudomonas aeruginosa	126:167	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	1	28	theme	virulent	180:187	arg1	pathogens					203:211	highly virulent opportunistic pathogens	173:211	highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance	173:281	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	5	29	theme	lung	818:821	arg1	infections					823:832	cystic fibrosis lung infections	802:832	cystic fibrosis lung infections	802:832	We also examined changes in PG composition when the strains were cultured under nutrient conditions that resembled cystic fibrosis lung infections.					
35545925	1	30	with	pathogens	203:211	arg1	transmissibility					228:243	increased transmissibility	218:243	increased transmissibility	218:243	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	1	30	with	pathogens	203:211	arg1	resistance					272:281	enhanced antimicrobial resistance	249:281	enhanced antimicrobial resistance	249:281	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	3	31	theme	P.	430:431	arg1	cells					444:448	P. aeruginosa cells	430:448	P. aeruginosa cells	430:448	Peptidoglycan (PG) is an integral component of P. aeruginosa cells that is essential to its survival and a target for antimicrobials.					
35545925	2	32	theme	heightened	334:343	arg1	virulence					345:353	virulence	345:353	virulence	345:353	Understanding the cellular mechanisms behind this heightened virulence and resistance is critical.					
35545925	14	33	theme	strains	2493:2499	arg1	composition					2446:2456	the PG composition	2439:2456	the PG composition of clinical P. aeruginosa epidemic strains	2439:2499	Analyzing the PG composition of clinical P. aeruginosa epidemic strains provides insights into the increased virulence and antimicrobial resistance of these difficult-to-eradicate infections.					
35545925	8	34	theme	stem	1374:1377	arg1	peptides					1379:1386	O-methylated stem peptides	1361:1386	O-methylated stem peptides	1361:1386	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	5	35	theme	nutrient	767:774	arg1	conditions					776:785	nutrient conditions	767:785	nutrient conditions that resembled cystic fibrosis lung infections	767:832	We also examined changes in PG composition when the strains were cultured under nutrient conditions that resembled cystic fibrosis lung infections.					
35545925	8	36	theme	lung	1323:1326	arg1	infections					1328:1337	cystic fibrosis (CF) lung infections	1302:1337	cystic fibrosis (CF) lung infections	1302:1337	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	14	37	dep	P.	2470:2471	arg1	aeruginosa					2473:2482	aeruginosa	2473:2482	aeruginosa	2473:2482	Analyzing the PG composition of clinical P. aeruginosa epidemic strains provides insights into the increased virulence and antimicrobial resistance of these difficult-to-eradicate infections.					
35545925	4	38	theme	global	539:544	arg1	composition					549:559	the global PG composition	535:559	the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1,	535:619	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	0	39	dep	Fibrosis	100:107	arg1	Infections					114:123	Lung Infections	109:123	Cystic Fibrosis Lung Infections	93:123	Pseudomonas aeruginosa Alters Peptidoglycan Composition under Nutrient Conditions Resembling Cystic Fibrosis Lung Infections.					
35545925	3	40	theme	cells	444:448	arg1	Peptidoglycan					383:395	Peptidoglycan	383:395	Peptidoglycan (PG)	383:400	Peptidoglycan (PG) is an integral component of P. aeruginosa cells that is essential to its survival and a target for antimicrobials.					
35545925	3	40	theme	cells	444:448	arg1	essential					458:466	essential	458:466	essential	458:466	Peptidoglycan (PG) is an integral component of P. aeruginosa cells that is essential to its survival and a target for antimicrobials.					
35545925	3	40	theme	cells	444:448	arg1	component					417:425	an integral component	405:425	an integral component of P. aeruginosa cells that is essential to its survival and a target for antimicrobials	405:514	Peptidoglycan (PG) is an integral component of P. aeruginosa cells that is essential to its survival and a target for antimicrobials.					
35545925	8	41	theme	antibiotic	1570:1579	arg1	resistance					1581:1590	antibiotic resistance	1570:1590	antibiotic resistance	1570:1590	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	7	42	theme	organism	1189:1196	arg1	sacculus					1161:1168	sacculus	1161:1168	sacculus	1161:1168	Our results also present the first evidence for both d,l- and l,d-endopeptidase activity on the PG sacculus of a Gram-negative organism.					
35545925	4	43	dep	P.	568:569	arg1	aeruginosa					571:580	aeruginosa	571:580	aeruginosa	571:580	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	6	44	theme	stem	908:911	arg1	peptides					913:920	stem peptides	908:920	stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG	908:1059	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	7	45	from	activity	1142:1149	arg1	sacculus					1161:1168	sacculus	1161:1168	sacculus	1161:1168	Our results also present the first evidence for both d,l- and l,d-endopeptidase activity on the PG sacculus of a Gram-negative organism.					
35545925	0	46	theme	Nutrient	62:69	arg1	Conditions					71:80	Nutrient Conditions	62:80	Nutrient Conditions Resembling Cystic Fibrosis Lung Infections	62:123	Pseudomonas aeruginosa Alters Peptidoglycan Composition under Nutrient Conditions Resembling Cystic Fibrosis Lung Infections.					
35545925	4	47	theme	strains	591:597	arg1	composition					549:559	the global PG composition	535:559	the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1,	535:619	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	12	48	theme	numerous	2219:2226	arg1	processes					2242:2250	numerous physiological processes	2219:2250	numerous physiological processes	2219:2250	Changes within the PG structure have implications for numerous physiological processes, including virulence and antimicrobial resistance.					
35545925	12	48	theme	numerous	2219:2226	arg1	virulence					2263:2271	virulence	2263:2271	virulence	2263:2271	Changes within the PG structure have implications for numerous physiological processes, including virulence and antimicrobial resistance.					
35545925	12	48	theme	numerous	2219:2226	arg1	resistance					2291:2300	antimicrobial resistance	2277:2300	antimicrobial resistance	2277:2300	Changes within the PG structure have implications for numerous physiological processes, including virulence and antimicrobial resistance.					
35545925	13	49	theme	modifications	2342:2354	arg1	identification					2307:2320	The identification	2303:2320	The identification of highly unique PG modifications	2303:2354	The identification of highly unique PG modifications illustrates the complexity of this biopolymer in Pseudomonas aeruginosa.					
35545925	14	50	theme	increased	2528:2536	arg1	virulence					2538:2546	virulence	2538:2546	virulence	2538:2546	Analyzing the PG composition of clinical P. aeruginosa epidemic strains provides insights into the increased virulence and antimicrobial resistance of these difficult-to-eradicate infections.					
35545925	4	51	theme	laboratory	653:662	arg1	PA14					681:684	PA14	681:684	PA14	681:684	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	4	51	theme	laboratory	653:662	arg1	PAO1					672:675	PAO1	672:675	PAO1	672:675	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	4	51	theme	laboratory	653:662	arg1	strains					664:670	the common laboratory strains	642:670	the common laboratory strains PAO1 and PA14	642:684	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	7	52	dep	d	1115:1115	arg1	activity					1142:1149	d-endopeptidase activity	1126:1149	d-endopeptidase activity on the PG sacculus of a Gram-negative organism	1126:1196	Our results also present the first evidence for both d,l- and l,d-endopeptidase activity on the PG sacculus of a Gram-negative organism.					
35545925	5	53	theme	fibrosis	809:816	arg1	infections					823:832	cystic fibrosis lung infections	802:832	cystic fibrosis lung infections	802:832	We also examined changes in PG composition when the strains were cultured under nutrient conditions that resembled cystic fibrosis lung infections.					
35545925	9	54	theme	amino	1698:1702	arg1	acids					1704:1708	unique amino acids	1691:1708	unique amino acids	1691:1708	We also identified strain-specific changes where LESlike1 increased the addition of unique amino acids to the terminus of the stem peptide and LESB58 increased amidase activity.					
35545925	10	55	theme	inherent	1932:1939	arg1	differences					1958:1968	inherent strain-to-strain differences	1932:1968	inherent strain-to-strain differences	1932:1968	Overall, this study demonstrates that P. aeruginosa PG composition is primarily influenced by nutrient conditions that mimic the CF lung; however, inherent strain-to-strain differences also exist.					
35545925	7	56	theme	d-endopeptidase	1126:1140	arg1	activity					1142:1149	d-endopeptidase activity	1126:1149	d-endopeptidase activity on the PG sacculus of a Gram-negative organism	1126:1196	Our results also present the first evidence for both d,l- and l,d-endopeptidase activity on the PG sacculus of a Gram-negative organism.					
35545925	8	57	from	decreases	1392:1400	arg1	peptides					1379:1386	O-methylated stem peptides	1361:1386	O-methylated stem peptides	1361:1386	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	8	57	from	decreases	1392:1400	arg1	l					1405:1405	l	1405:1405	l	1405:1405	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	6	58	theme	meso-diaminopimelic	951:969	arg1	deamination					983:993	meso-diaminopimelic acid (mDAP) deamination	951:993	meso-diaminopimelic acid (mDAP) deamination	951:993	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	8	59	dep	activity	1423:1430	arg1	l					1405:1405	l	1405:1405	l	1405:1405	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	14	60	theme	epidemic	2484:2491	arg1	strains					2493:2499	clinical P. aeruginosa epidemic strains	2461:2499	clinical P. aeruginosa epidemic strains	2461:2499	Analyzing the PG composition of clinical P. aeruginosa epidemic strains provides insights into the increased virulence and antimicrobial resistance of these difficult-to-eradicate infections.					
35545925	11	61	theme	peptidoglycan	2057:2069	arg1	composition					2038:2048	the global composition	2027:2048	the global composition of the peptidoglycan (PG)	2027:2074	IMPORTANCE Using peptidoglycomics to examine the global composition of the peptidoglycan (PG) allows insights into the enzymatic activity that functions on this important biopolymer.					
35545925	14	62	theme	difficult-to-eradicate	2586:2607	arg1	infections					2609:2618	these difficult-to-eradicate infections	2580:2618	these difficult-to-eradicate infections	2580:2618	Analyzing the PG composition of clinical P. aeruginosa epidemic strains provides insights into the increased virulence and antimicrobial resistance of these difficult-to-eradicate infections.					
35545925	12	63	theme	PG	2184:2185	arg1	structure					2187:2195	the PG structure	2180:2195	the PG structure	2180:2195	Changes within the PG structure have implications for numerous physiological processes, including virulence and antimicrobial resistance.					
35545925	14	64	theme	P.	2470:2471	arg1	strains					2493:2499	clinical P. aeruginosa epidemic strains	2461:2499	clinical P. aeruginosa epidemic strains	2461:2499	Analyzing the PG composition of clinical P. aeruginosa epidemic strains provides insights into the increased virulence and antimicrobial resistance of these difficult-to-eradicate infections.					
35545925	8	65	theme	PG	1203:1204	arg1	composition					1206:1216	The PG composition	1199:1216	The PG composition of the epidemic strains	1199:1240	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	6	66	theme	residues	1028:1035	arg1	deamination					983:993	meso-diaminopimelic acid (mDAP) deamination	951:993	meso-diaminopimelic acid (mDAP) deamination	951:993	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	6	66	theme	residues	1028:1035	arg1	O-methylation					936:948	O-methylation	936:948	O-methylation	936:948	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	6	66	theme	residues	1028:1035	arg1	substitutions					1006:1018	novel substitutions	1000:1018	novel substitutions of mDAP residues within P. aeruginosa PG	1000:1059	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	14	67	theme	clinical	2461:2468	arg1	strains					2493:2499	clinical P. aeruginosa epidemic strains	2461:2499	clinical P. aeruginosa epidemic strains	2461:2499	Analyzing the PG composition of clinical P. aeruginosa epidemic strains provides insights into the increased virulence and antimicrobial resistance of these difficult-to-eradicate infections.					
35545925	1	68	theme	enhanced	249:256	arg1	resistance					272:281	enhanced antimicrobial resistance	249:281	enhanced antimicrobial resistance	249:281	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	9	69	theme	peptide	1738:1744	arg1	terminus					1717:1724	the terminus	1713:1724	the terminus of the stem peptide	1713:1744	We also identified strain-specific changes where LESlike1 increased the addition of unique amino acids to the terminus of the stem peptide and LESB58 increased amidase activity.					
35545925	8	70	theme	de-N-acetylated	1469:1483	arg1	sugars					1485:1490	de-N-acetylated sugars	1469:1490	de-N-acetylated sugars	1469:1490	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	8	71	theme	epidemic	1225:1232	arg1	strains					1234:1240	the epidemic strains	1221:1240	the epidemic strains	1221:1240	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	6	72	theme	novel	1000:1004	arg1	substitutions					1006:1018	novel substitutions	1000:1018	novel substitutions of mDAP residues within P. aeruginosa PG	1000:1059	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	8	73	theme	fibrosis	1309:1316	arg1	infections					1328:1337	cystic fibrosis (CF) lung infections	1302:1337	cystic fibrosis (CF) lung infections	1302:1337	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	7	74	theme	first	1091:1095	arg1	evidence					1097:1104	the first evidence	1087:1104	the first evidence for both d,l- and l,d-endopeptidase activity on the PG sacculus of a Gram-negative organism	1087:1196	Our results also present the first evidence for both d,l- and l,d-endopeptidase activity on the PG sacculus of a Gram-negative organism.					
35545925	9	75	theme	amidase	1767:1773	arg1	activity					1775:1782	amidase activity	1767:1782	amidase activity	1767:1782	We also identified strain-specific changes where LESlike1 increased the addition of unique amino acids to the terminus of the stem peptide and LESB58 increased amidase activity.					
35545925	7	76	theme	Gram-negative	1175:1187	arg1	organism					1189:1196	a Gram-negative organism	1173:1196	a Gram-negative organism	1173:1196	Our results also present the first evidence for both d,l- and l,d-endopeptidase activity on the PG sacculus of a Gram-negative organism.					
35545925	1	77	theme	aeruginosa	158:167	arg1	pathogens					203:211	highly virulent opportunistic pathogens	173:211	highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance	173:281	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	1	77	theme	aeruginosa	158:167	arg1	strains					135:141	Epidemic strains	126:141	Epidemic strains of Pseudomonas aeruginosa	126:167	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	10	78	dep	P.	1823:1824	arg1	aeruginosa					1826:1835	aeruginosa	1826:1835	aeruginosa	1826:1835	Overall, this study demonstrates that P. aeruginosa PG composition is primarily influenced by nutrient conditions that mimic the CF lung; however, inherent strain-to-strain differences also exist.					
35545925	0	79	theme	Cystic	93:98	arg1	Fibrosis					100:107	Cystic Fibrosis Lung Infections	93:123	Cystic Fibrosis Lung Infections	93:123	Pseudomonas aeruginosa Alters Peptidoglycan Composition under Nutrient Conditions Resembling Cystic Fibrosis Lung Infections.					
35545925	11	80	theme	global	2031:2036	arg1	composition					2038:2048	the global composition	2027:2048	the global composition of the peptidoglycan (PG)	2027:2074	IMPORTANCE Using peptidoglycomics to examine the global composition of the peptidoglycan (PG) allows insights into the enzymatic activity that functions on this important biopolymer.					
35545925	13	81	theme	PG	2339:2340	arg1	modifications					2342:2354	highly unique PG modifications	2325:2354	highly unique PG modifications	2325:2354	The identification of highly unique PG modifications illustrates the complexity of this biopolymer in Pseudomonas aeruginosa.					
35545925	3	82	theme	integral	408:415	arg1	Peptidoglycan					383:395	Peptidoglycan	383:395	Peptidoglycan (PG)	383:400	Peptidoglycan (PG) is an integral component of P. aeruginosa cells that is essential to its survival and a target for antimicrobials.					
35545925	3	82	theme	integral	408:415	arg1	essential					458:466	essential	458:466	essential	458:466	Peptidoglycan (PG) is an integral component of P. aeruginosa cells that is essential to its survival and a target for antimicrobials.					
35545925	3	82	theme	integral	408:415	arg1	component					417:425	an integral component	405:425	an integral component of P. aeruginosa cells that is essential to its survival and a target for antimicrobials	405:514	Peptidoglycan (PG) is an integral component of P. aeruginosa cells that is essential to its survival and a target for antimicrobials.					
35545925	6	83	dep	P.	1044:1045	arg1	aeruginosa					1047:1056	aeruginosa	1047:1056	aeruginosa	1047:1056	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	0	84	theme	Lung	109:112	arg1	Infections					114:123	Lung Infections	109:123	Cystic Fibrosis Lung Infections	93:123	Pseudomonas aeruginosa Alters Peptidoglycan Composition under Nutrient Conditions Resembling Cystic Fibrosis Lung Infections.					
35545925	1	85	theme	opportunistic	189:201	arg1	strains					135:141	Epidemic strains	126:141	Epidemic strains of Pseudomonas aeruginosa	126:167	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	1	85	theme	opportunistic	189:201	arg1	pathogens					203:211	highly virulent opportunistic pathogens	173:211	highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance	173:281	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	4	86	theme	P.	568:569	arg1	strains					591:597	two P. aeruginosa epidemic strains	564:597	two P. aeruginosa epidemic strains	564:597	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	4	86	theme	P.	568:569	arg1	LESlike1					611:618	LESlike1	611:618	LESlike1	611:618	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	4	86	theme	P.	568:569	arg1	LESB58					600:605	LESB58	600:605	LESB58	600:605	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	6	87	theme	P.	1044:1045	arg1	PG					1058:1059	P. aeruginosa PG	1044:1059	P. aeruginosa PG	1044:1059	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	3	88	theme	aeruginosa	433:442	arg1	cells					444:448	P. aeruginosa cells	430:448	P. aeruginosa cells	430:448	Peptidoglycan (PG) is an integral component of P. aeruginosa cells that is essential to its survival and a target for antimicrobials.					
35545925	8	89	theme	bacterial	1546:1554	arg1	virulence					1556:1564	bacterial virulence	1546:1564	bacterial virulence	1546:1564	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	8	90	theme	O-methylated	1361:1372	arg1	peptides					1379:1386	O-methylated stem peptides	1361:1386	O-methylated stem peptides	1361:1386	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	4	91	theme	PG	546:547	arg1	composition					549:559	the global PG composition	535:559	the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1,	535:619	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	8	92	from	increases	1348:1356	arg1	peptides					1379:1386	O-methylated stem peptides	1361:1386	O-methylated stem peptides	1361:1386	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	8	92	from	increases	1348:1356	arg1	l					1405:1405	l	1405:1405	l	1405:1405	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	12	93	theme	antimicrobial	2277:2289	arg1	resistance					2291:2300	antimicrobial resistance	2277:2300	antimicrobial resistance	2277:2300	Changes within the PG structure have implications for numerous physiological processes, including virulence and antimicrobial resistance.					
35545925	0	94	theme	Peptidoglycan	30:42	arg1	Composition					44:54	Peptidoglycan Composition	30:54	Peptidoglycan Composition	30:54	Pseudomonas aeruginosa Alters Peptidoglycan Composition under Nutrient Conditions Resembling Cystic Fibrosis Lung Infections.					
35545925	1	95	theme	Epidemic	126:133	arg1	pathogens					203:211	highly virulent opportunistic pathogens	173:211	highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance	173:281	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	1	95	theme	Epidemic	126:133	arg1	strains					135:141	Epidemic strains	126:141	Epidemic strains of Pseudomonas aeruginosa	126:167	Epidemic strains of Pseudomonas aeruginosa are highly virulent opportunistic pathogens with increased transmissibility and enhanced antimicrobial resistance.					
35545925	10	96	theme	CF	1914:1915	arg1	lung					1917:1920	the CF lung	1910:1920	the CF lung	1910:1920	Overall, this study demonstrates that P. aeruginosa PG composition is primarily influenced by nutrient conditions that mimic the CF lung; however, inherent strain-to-strain differences also exist.					
35545925	13	97	theme	unique	2332:2337	arg1	modifications					2342:2354	highly unique PG modifications	2325:2354	highly unique PG modifications	2325:2354	The identification of highly unique PG modifications illustrates the complexity of this biopolymer in Pseudomonas aeruginosa.					
35545925	9	98	theme	strain-specific	1626:1640	arg1	changes					1642:1648	strain-specific changes	1626:1648	strain-specific changes where LESlike1 increased the addition of unique amino acids to the terminus of the stem peptide and LESB58 increased amidase activity	1626:1782	We also identified strain-specific changes where LESlike1 increased the addition of unique amino acids to the terminus of the stem peptide and LESB58 increased amidase activity.					
35545925	4	99	theme	epidemic	582:589	arg1	strains					591:597	two P. aeruginosa epidemic strains	564:597	two P. aeruginosa epidemic strains	564:597	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	4	99	theme	epidemic	582:589	arg1	LESlike1					611:618	LESlike1	611:618	LESlike1	611:618	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	4	99	theme	epidemic	582:589	arg1	LESB58					600:605	LESB58	600:605	LESB58	600:605	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	8	100	theme	d-endopeptidase	1407:1421	arg1	activity					1423:1430	d-endopeptidase activity	1407:1430	d-endopeptidase activity	1407:1430	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	5	101	from	changes	704:710	arg1	composition					718:728	PG composition	715:728	PG composition	715:728	We also examined changes in PG composition when the strains were cultured under nutrient conditions that resembled cystic fibrosis lung infections.					
35545925	14	102	theme	antimicrobial	2552:2564	arg1	resistance					2566:2575	antimicrobial resistance	2552:2575	antimicrobial resistance	2552:2575	Analyzing the PG composition of clinical P. aeruginosa epidemic strains provides insights into the increased virulence and antimicrobial resistance of these difficult-to-eradicate infections.					
35545925	12	103	theme	physiological	2228:2240	arg1	processes					2242:2250	numerous physiological processes	2219:2250	numerous physiological processes	2219:2250	Changes within the PG structure have implications for numerous physiological processes, including virulence and antimicrobial resistance.					
35545925	12	103	theme	physiological	2228:2240	arg1	virulence					2263:2271	virulence	2263:2271	virulence	2263:2271	Changes within the PG structure have implications for numerous physiological processes, including virulence and antimicrobial resistance.					
35545925	12	103	theme	physiological	2228:2240	arg1	resistance					2291:2300	antimicrobial resistance	2277:2300	antimicrobial resistance	2277:2300	Changes within the PG structure have implications for numerous physiological processes, including virulence and antimicrobial resistance.					
35545925	6	104	theme	first	889:893	arg1	evidence					895:902	the first evidence	885:902	the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG	885:1059	We identified 448 unique muropeptides and provide the first evidence for stem peptides modified with O-methylation, meso-diaminopimelic acid (mDAP) deamination, and novel substitutions of mDAP residues within P. aeruginosa PG.					
35545925	4	105	theme	common	646:651	arg1	PA14					681:684	PA14	681:684	PA14	681:684	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	4	105	theme	common	646:651	arg1	PAO1					672:675	PAO1	672:675	PAO1	672:675	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	4	105	theme	common	646:651	arg1	strains					664:670	the common laboratory strains	642:670	the common laboratory strains PAO1 and PA14	642:684	Here, we examined the global PG composition of two P. aeruginosa epidemic strains, LESB58 and LESlike1, and compared them to the common laboratory strains PAO1 and PA14.					
35545925	8	106	theme	increased	1446:1454	arg1	related					1535:1541	related	1535:1541	related	1535:1541	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
35545925	8	106	theme	increased	1446:1454	arg1	abundance					1456:1464	an increased abundance	1443:1464	an increased abundance of de-N-acetylated sugars	1443:1490	The PG composition of the epidemic strains varied significantly when grown under conditions resembling cystic fibrosis (CF) lung infections, showing increases in O-methylated stem peptides and decreases in l,d-endopeptidase activity as well as an increased abundance of de-N-acetylated sugars and l,d-transpeptidase activity, which are related to bacterial virulence and antibiotic resistance, respectively.					
36099110	5	0	from	composition	748:758	arg1	SA					821:822	SA	821:822	SA	821:822	ECM composition of hyaluronic acid (HA) and collagen in the subglottic area (SA) was also evaluated.					
36099110	5	0	from	composition	748:758	arg1	area					815:818	the subglottic area	800:818	the subglottic area (SA)	800:823	ECM composition of hyaluronic acid (HA) and collagen in the subglottic area (SA) was also evaluated.					
36099110	2	1	from	changes	182:188	arg1	markers					236:242	markers	236:242	markers for inflammation and microcirculation of laryngeal mucosa	236:300	We investigated the changes in extracellular matrix (ECM) composition and markers for inflammation and microcirculation of laryngeal mucosa.					
36099110	2	1	from	changes	182:188	arg1	composition					220:230	extracellular matrix (ECM) composition	193:230	extracellular matrix (ECM) composition	193:230	We investigated the changes in extracellular matrix (ECM) composition and markers for inflammation and microcirculation of laryngeal mucosa.					
36099110	9	2	theme	surgical	1344:1351	arg1	extent					1353:1358	the surgical extent	1340:1358	the surgical extent	1340:1358	VEGFR-3 and CD31 expression increased with time at all postoperative phases according to the surgical extent.					
36099110	5	3	theme	hyaluronic	763:772	arg1	acid					774:777	hyaluronic acid	763:777	hyaluronic acid (HA)	763:782	ECM composition of hyaluronic acid (HA) and collagen in the subglottic area (SA) was also evaluated.					
36099110	5	3	theme	hyaluronic	763:772	arg1	HA					780:781	HA	780:781	HA	780:781	ECM composition of hyaluronic acid (HA) and collagen in the subglottic area (SA) was also evaluated.					
36099110	4	4	from	expression	570:579	arg1	relation					621:628	relation	621:628	relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21	621:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	12	5	theme	laryngeal	1732:1740	arg1	mucosa					1742:1747	the laryngeal mucosa	1728:1747	the laryngeal mucosa	1728:1747	Furthermore, the markers for acute inflammation and microcirculation with tissue remodeling increased in the laryngeal mucosa.					
36099110	7	6	from	control	1076:1082	arg1	POD					1110:1112	POD 21	1110:1115	POD 21	1110:1115	On POD 3, TGF-β1 expression and SA increased in relation to the surgical extent and decreased over time, but more than the control in all surgical groups on POD 21.					
36099110	7	6	from	control	1076:1082	arg1	groups					1100:1105	all surgical groups	1087:1105	all surgical groups	1087:1105	On POD 3, TGF-β1 expression and SA increased in relation to the surgical extent and decreased over time, but more than the control in all surgical groups on POD 21.					
36099110	4	7	theme	TGF-β1	584:589	arg1	expression					570:579	the expression	566:579	the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21	566:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	2	8	theme	laryngeal	285:293	arg1	mucosa					295:300	laryngeal mucosa	285:300	laryngeal mucosa	285:300	We investigated the changes in extracellular matrix (ECM) composition and markers for inflammation and microcirculation of laryngeal mucosa.					
36099110	5	9	theme	acid	774:777	arg1	composition					748:758	ECM composition	744:758	ECM composition of hyaluronic acid (HA)	744:782	ECM composition of hyaluronic acid (HA) and collagen in the subglottic area (SA) was also evaluated.					
36099110	5	9	theme	acid	774:777	arg1	collagen					788:795	collagen	788:795	collagen in the subglottic area (SA)	788:823	ECM composition of hyaluronic acid (HA) and collagen in the subglottic area (SA) was also evaluated.					
36099110	3	10	theme	thyroid	522:528	arg1	TI					543:544	TI	543:544	TI	543:544	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	3	10	theme	thyroid	522:528	arg1	isthmectomy					530:540	3) thyroid isthmectomy	519:540	3) thyroid isthmectomy (TI) group	519:551	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	2	11	theme	matrix	207:212	arg1	composition					220:230	extracellular matrix (ECM) composition	193:230	extracellular matrix (ECM) composition	193:230	We investigated the changes in extracellular matrix (ECM) composition and markers for inflammation and microcirculation of laryngeal mucosa.					
36099110	8	12	dep	ratio	1209:1213	arg1	to					1197:1198	to	1197:1198	to	1197:1198	Surgical groups had more HA and less collagen composition, causing a higher HA to collagen ratio in relation to the surgical extent.					
36099110	4	13	from	propria	693:699	arg1	PODs					724:727	PODs	724:727	PODs	724:727	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	4	13	from	propria	693:699	arg1	days					718:721	postoperative days	704:721	postoperative days (PODs) 3, 7, and 21	704:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	3	14	theme	Sixty	325:329	arg1	rats					346:349	MATERIALS AND METHODS Sixty Sprague-Dawley rats	303:349	MATERIALS AND METHODS Sixty Sprague-Dawley rats	303:349	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	4	15	theme	VEGFR-3	592:598	arg1	expression					570:579	the expression	566:579	the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21	566:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	11	16	theme	thyroid	1494:1500	arg1	surgery					1502:1508	thyroid surgery	1494:1508	thyroid surgery exposing the thyroid and trachea	1494:1541	CONCLUSION This study demonstrated that thyroid surgery exposing the thyroid and trachea induces an increase in the SA with a higher HA and lesser collagen composition.					
36099110	5	17	theme	ECM	744:746	arg1	composition					748:758	ECM composition	744:758	ECM composition of hyaluronic acid (HA)	744:782	ECM composition of hyaluronic acid (HA) and collagen in the subglottic area (SA) was also evaluated.					
36099110	4	18	theme	CD31	601:604	arg1	expression					570:579	the expression	566:579	the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21	566:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	0	19	theme	Rat	57:59	arg1	Model					61:65	A Rat Model	55:65	A Rat Model	55:65	Changes in the Laryngeal Mucosa After Thyroid Surgery: A Rat Model.					
36099110	4	20	from	changes	671:677	arg1	PODs					724:727	PODs	724:727	PODs	724:727	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	4	20	from	changes	671:677	arg1	propria					693:699	the lamina propria	682:699	the lamina propria on postoperative days (PODs) 3, 7, and 21	682:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	4	20	from	changes	671:677	arg1	days					718:721	postoperative days	704:721	postoperative days (PODs) 3, 7, and 21	704:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	9	21	theme	postoperative	1306:1318	arg1	phases					1320:1325	all postoperative phases	1302:1325	all postoperative phases according to the surgical extent	1302:1358	VEGFR-3 and CD31 expression increased with time at all postoperative phases according to the surgical extent.					
36099110	0	22	from	Changes	0:6	arg1	Mucosa					25:30	the Laryngeal Mucosa	11:30	the Laryngeal Mucosa	11:30	Changes in the Laryngeal Mucosa After Thyroid Surgery: A Rat Model.					
36099110	5	23	theme	subglottic	804:813	arg1	SA					821:822	SA	821:822	SA	821:822	ECM composition of hyaluronic acid (HA) and collagen in the subglottic area (SA) was also evaluated.					
36099110	5	23	theme	subglottic	804:813	arg1	area					815:818	the subglottic area	800:818	the subglottic area (SA)	800:823	ECM composition of hyaluronic acid (HA) and collagen in the subglottic area (SA) was also evaluated.					
36099110	8	24	contain	had	1134:1136	arg2	composition					1164:1174	more HA and less collagen composition	1138:1174	more HA and less collagen composition	1138:1174	Surgical groups had more HA and less collagen composition, causing a higher HA to collagen ratio in relation to the surgical extent.					
36099110	8	24	contain	had	1134:1136	arg1	groups					1127:1132	Surgical groups	1118:1132	Surgical groups	1118:1132	Surgical groups had more HA and less collagen composition, causing a higher HA to collagen ratio in relation to the surgical extent.					
36099110	3	25	theme	surgeries	430:438	arg1	extent					420:425	the extent	416:425	the extent of surgeries	416:438	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	6	26	dep	RESULTS	845:851	arg1	parameters					857:866	All parameters	853:866	RESULTS All parameters	845:866	RESULTS All parameters increased in surgical groups at each postoperative phase except collagen deposition.					
36099110	8	27	theme	collagen	1155:1162	arg1	composition					1164:1174	more HA and less collagen composition	1138:1174	more HA and less collagen composition	1138:1174	Surgical groups had more HA and less collagen composition, causing a higher HA to collagen ratio in relation to the surgical extent.					
36099110	1	28	theme	BACKGROUND/AIM	68:81	arg1	Thyroidectomy					83:95	BACKGROUND/AIM Thyroidectomy	68:95	BACKGROUND/AIM Thyroidectomy	68:95	BACKGROUND/AIM Thyroidectomy can cause various airway symptoms affecting the quality of life.					
36099110	3	29	theme	flap	444:447	arg1	group					464:468	1) flap elevation (FE) group	441:468	1) flap elevation (FE) group	441:468	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	3	29	theme	flap	444:447	arg1	groups					400:405	three surgical groups	385:405	three surgical groups based on the extent of surgeries	385:438	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	3	30	dep	thyroid	474:480	arg1	2					471:471	2	471:471	2	471:471	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	3	30	dep	thyroid	474:480	arg1	group					508:512	exposure (TE) group	494:512	exposure (TE) group	494:512	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	4	31	theme	postoperative	704:716	arg1	PODs					724:727	PODs	724:727	PODs	724:727	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	4	31	theme	postoperative	704:716	arg1	days					718:721	postoperative days	704:721	postoperative days (PODs) 3, 7, and 21	704:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	8	32	theme	HA	1194:1195	arg1	ratio					1209:1213	a higher HA to collagen ratio	1185:1213	a higher HA to collagen ratio	1185:1213	Surgical groups had more HA and less collagen composition, causing a higher HA to collagen ratio in relation to the surgical extent.					
36099110	11	33	theme	lesser	1594:1599	arg1	composition					1610:1620	lesser collagen composition	1594:1620	lesser collagen composition	1594:1620	CONCLUSION This study demonstrated that thyroid surgery exposing the thyroid and trachea induces an increase in the SA with a higher HA and lesser collagen composition.					
36099110	7	34	theme	TGF-β1	963:968	arg1	expression					970:979	TGF-β1 expression	963:979	TGF-β1 expression	963:979	On POD 3, TGF-β1 expression and SA increased in relation to the surgical extent and decreased over time, but more than the control in all surgical groups on POD 21.					
36099110	10	35	from	groups	1426:1431	arg1	POD					1436:1438	POD 7	1436:1440	POD 7	1436:1440	Expression of MMP-9 increased in TI groups compared to TE and FE groups on POD 7 and POD 21.					
36099110	10	35	from	groups	1426:1431	arg1	POD					1446:1448	POD 21	1446:1451	POD 21	1446:1451	Expression of MMP-9 increased in TI groups compared to TE and FE groups on POD 7 and POD 21.					
36099110	8	36	theme	collagen	1200:1207	arg1	ratio					1209:1213	a higher HA to collagen ratio	1185:1213	a higher HA to collagen ratio	1185:1213	Surgical groups had more HA and less collagen composition, causing a higher HA to collagen ratio in relation to the surgical extent.					
36099110	6	37	from	phase	919:923	arg1	groups					890:895	surgical groups	881:895	surgical groups at each postoperative phase except collagen deposition	881:950	RESULTS All parameters increased in surgical groups at each postoperative phase except collagen deposition.					
36099110	6	38	theme	collagen	932:939	arg1	deposition					941:950	collagen deposition	932:950	collagen deposition	932:950	RESULTS All parameters increased in surgical groups at each postoperative phase except collagen deposition.					
36099110	3	39	theme	exposure	494:501	arg1	group					508:512	exposure (TE) group	494:512	exposure (TE) group	494:512	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	1	40	theme	life	156:159	arg1	quality					145:151	the quality	141:151	the quality of life	141:159	BACKGROUND/AIM Thyroidectomy can cause various airway symptoms affecting the quality of life.					
36099110	0	41	theme	Thyroid	38:44	arg1	Surgery					46:52	Thyroid Surgery	38:52	Thyroid Surgery	38:52	Changes in the Laryngeal Mucosa After Thyroid Surgery: A Rat Model.					
36099110	11	42	from	increase	1554:1561	arg1	SA					1570:1571	the SA	1566:1571	the SA with a higher HA and lesser collagen composition	1566:1620	CONCLUSION This study demonstrated that thyroid surgery exposing the thyroid and trachea induces an increase in the SA with a higher HA and lesser collagen composition.					
36099110	10	43	theme	MMP-9	1375:1379	arg1	Expression					1361:1370	Expression	1361:1370	Expression of MMP-9	1361:1379	Expression of MMP-9 increased in TI groups compared to TE and FE groups on POD 7 and POD 21.					
36099110	3	44	theme	TE	504:505	arg1	group					508:512	exposure (TE) group	494:512	exposure (TE) group	494:512	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	11	45	with	SA	1570:1571	arg1	HA					1587:1588	a higher HA	1578:1588	a higher HA	1578:1588	CONCLUSION This study demonstrated that thyroid surgery exposing the thyroid and trachea induces an increase in the SA with a higher HA and lesser collagen composition.					
36099110	11	45	with	SA	1570:1571	arg1	composition					1610:1620	lesser collagen composition	1594:1620	lesser collagen composition	1594:1620	CONCLUSION This study demonstrated that thyroid surgery exposing the thyroid and trachea induces an increase in the SA with a higher HA and lesser collagen composition.					
36099110	3	46	dep	isthmectomy	530:540	arg1	3					519:519	3	519:519	3	519:519	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	12	47	theme	tissue	1697:1702	arg1	remodeling					1704:1713	tissue remodeling	1697:1713	tissue remodeling	1697:1713	Furthermore, the markers for acute inflammation and microcirculation with tissue remodeling increased in the laryngeal mucosa.					
36099110	8	48	theme	surgical	1234:1241	arg1	extent					1243:1248	the surgical extent	1230:1248	the surgical extent	1230:1248	Surgical groups had more HA and less collagen composition, causing a higher HA to collagen ratio in relation to the surgical extent.					
36099110	11	49	theme	higher	1580:1585	arg1	HA					1587:1588	a higher HA	1578:1588	a higher HA	1578:1588	CONCLUSION This study demonstrated that thyroid surgery exposing the thyroid and trachea induces an increase in the SA with a higher HA and lesser collagen composition.					
36099110	4	50	from	days	718:721	arg1	changes					671:677	the inflammatory and microcirculatory changes	633:677	the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21	633:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	9	51	theme	CD31	1263:1266	arg1	expression					1268:1277	CD31 expression	1263:1277	CD31 expression	1263:1277	VEGFR-3 and CD31 expression increased with time at all postoperative phases according to the surgical extent.					
36099110	3	52	theme	MATERIALS	303:311	arg1	rats					346:349	MATERIALS AND METHODS Sixty Sprague-Dawley rats	303:349	MATERIALS AND METHODS Sixty Sprague-Dawley rats	303:349	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	1	53	theme	airway	115:120	arg1	symptoms					122:129	various airway symptoms	107:129	various airway symptoms affecting the quality of life	107:159	BACKGROUND/AIM Thyroidectomy can cause various airway symptoms affecting the quality of life.					
36099110	2	54	theme	mucosa	295:300	arg1	microcirculation					265:280	microcirculation	265:280	microcirculation	265:280	We investigated the changes in extracellular matrix (ECM) composition and markers for inflammation and microcirculation of laryngeal mucosa.					
36099110	2	54	theme	mucosa	295:300	arg1	inflammation					248:259	inflammation	248:259	inflammation	248:259	We investigated the changes in extracellular matrix (ECM) composition and markers for inflammation and microcirculation of laryngeal mucosa.					
36099110	4	55	theme	MMP-	611:614	arg1	expression					570:579	the expression	566:579	the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21	566:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	3	56	theme	isthmectomy	530:540	arg1	group					547:551	3) thyroid isthmectomy (TI) group	519:551	3) thyroid isthmectomy (TI) group	519:551	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	3	56	theme	isthmectomy	530:540	arg1	groups					400:405	three surgical groups	385:405	three surgical groups based on the extent of surgeries	385:438	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	1	57	theme	various	107:113	arg1	symptoms					122:129	various airway symptoms	107:129	various airway symptoms affecting the quality of life	107:159	BACKGROUND/AIM Thyroidectomy can cause various airway symptoms affecting the quality of life.					
36099110	3	58	theme	Sprague-Dawley	331:344	arg1	rats					346:349	MATERIALS AND METHODS Sixty Sprague-Dawley rats	303:349	MATERIALS AND METHODS Sixty Sprague-Dawley rats	303:349	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	3	59	theme	METHODS	317:323	arg1	rats					346:349	MATERIALS AND METHODS Sixty Sprague-Dawley rats	303:349	MATERIALS AND METHODS Sixty Sprague-Dawley rats	303:349	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	8	60	theme	Surgical	1118:1125	arg1	groups					1127:1132	Surgical groups	1118:1132	Surgical groups	1118:1132	Surgical groups had more HA and less collagen composition, causing a higher HA to collagen ratio in relation to the surgical extent.					
36099110	7	61	theme	surgical	1017:1024	arg1	extent					1026:1031	the surgical extent	1013:1031	the surgical extent	1013:1031	On POD 3, TGF-β1 expression and SA increased in relation to the surgical extent and decreased over time, but more than the control in all surgical groups on POD 21.					
36099110	4	62	theme	inflammatory	637:648	arg1	changes					671:677	the inflammatory and microcirculatory changes	633:677	the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21	633:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	6	63	theme	postoperative	905:917	arg1	phase					919:923	each postoperative phase	900:923	each postoperative phase except collagen deposition	900:950	RESULTS All parameters increased in surgical groups at each postoperative phase except collagen deposition.					
36099110	4	64	theme	microcirculatory	654:669	arg1	changes					671:677	the inflammatory and microcirculatory changes	633:677	the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21	633:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	10	65	theme	FE	1423:1424	arg1	groups					1426:1431	TE and FE groups	1416:1431	groups	1426:1431	Expression of MMP-9 increased in TI groups compared to TE and FE groups on POD 7 and POD 21.					
36099110	12	66	theme	acute	1652:1656	arg1	inflammation					1658:1669	inflammation	1658:1669	inflammation	1658:1669	Furthermore, the markers for acute inflammation and microcirculation with tissue remodeling increased in the laryngeal mucosa.					
36099110	0	67	dep	Model	61:65	arg1	Changes					0:6	Changes	0:6	Changes in the Laryngeal Mucosa After Thyroid Surgery	0:52	Changes in the Laryngeal Mucosa After Thyroid Surgery: A Rat Model.					
36099110	3	68	theme	surgical	391:398	arg1	group					547:551	3) thyroid isthmectomy (TI) group	519:551	3) thyroid isthmectomy (TI) group	519:551	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	3	68	theme	surgical	391:398	arg1	group					464:468	1) flap elevation (FE) group	441:468	1) flap elevation (FE) group	441:468	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	3	68	theme	surgical	391:398	arg1	groups					400:405	three surgical groups	385:405	three surgical groups based on the extent of surgeries	385:438	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	3	68	theme	surgical	391:398	arg1	thyroid					474:480	thyroid	474:480	thyroid	474:480	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	8	69	theme	less	1150:1153	arg1	composition					1164:1174	more HA and less collagen composition	1138:1174	more HA and less collagen composition	1138:1174	Surgical groups had more HA and less collagen composition, causing a higher HA to collagen ratio in relation to the surgical extent.					
36099110	10	70	theme	TE	1416:1417	arg1	groups					1426:1431	TE and FE groups	1416:1431	groups	1426:1431	Expression of MMP-9 increased in TI groups compared to TE and FE groups on POD 7 and POD 21.					
36099110	0	71	theme	Laryngeal	15:23	arg1	Mucosa					25:30	the Laryngeal Mucosa	11:30	the Laryngeal Mucosa	11:30	Changes in the Laryngeal Mucosa After Thyroid Surgery: A Rat Model.					
36099110	12	72	with	microcirculation	1675:1690	arg1	remodeling					1704:1713	tissue remodeling	1697:1713	tissue remodeling	1697:1713	Furthermore, the markers for acute inflammation and microcirculation with tissue remodeling increased in the laryngeal mucosa.					
36099110	7	73	dep	more	1062:1065	arg1	control					1076:1082	the control	1072:1082	the control in all surgical groups on POD 21	1072:1115	On POD 3, TGF-β1 expression and SA increased in relation to the surgical extent and decreased over time, but more than the control in all surgical groups on POD 21.					
36099110	6	74	theme	surgical	881:888	arg1	groups					890:895	surgical groups	881:895	surgical groups at each postoperative phase except collagen deposition	881:950	RESULTS All parameters increased in surgical groups at each postoperative phase except collagen deposition.					
36099110	3	75	theme	elevation	449:457	arg1	group					464:468	1) flap elevation (FE) group	441:468	1) flap elevation (FE) group	441:468	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	3	75	theme	elevation	449:457	arg1	groups					400:405	three surgical groups	385:405	three surgical groups based on the extent of surgeries	385:438	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	12	76	with	inflammation	1658:1669	arg1	remodeling					1704:1713	tissue remodeling	1697:1713	tissue remodeling	1697:1713	Furthermore, the markers for acute inflammation and microcirculation with tissue remodeling increased in the laryngeal mucosa.					
36099110	7	77	theme	surgical	1091:1098	arg1	groups					1100:1105	all surgical groups	1087:1105	all surgical groups	1087:1105	On POD 3, TGF-β1 expression and SA increased in relation to the surgical extent and decreased over time, but more than the control in all surgical groups on POD 21.					
36099110	2	78	theme	extracellular	193:205	arg1	ECM					215:217	ECM	215:217	ECM	215:217	We investigated the changes in extracellular matrix (ECM) composition and markers for inflammation and microcirculation of laryngeal mucosa.					
36099110	2	78	theme	extracellular	193:205	arg1	matrix					207:212	extracellular matrix	193:212	extracellular matrix (ECM) composition	193:230	We investigated the changes in extracellular matrix (ECM) composition and markers for inflammation and microcirculation of laryngeal mucosa.					
36099110	8	79	theme	higher	1187:1192	arg1	ratio					1209:1213	a higher HA to collagen ratio	1185:1213	a higher HA to collagen ratio	1185:1213	Surgical groups had more HA and less collagen composition, causing a higher HA to collagen ratio in relation to the surgical extent.					
36099110	11	80	theme	collagen	1601:1608	arg1	composition					1610:1620	lesser collagen composition	1594:1620	lesser collagen composition	1594:1620	CONCLUSION This study demonstrated that thyroid surgery exposing the thyroid and trachea induces an increase in the SA with a higher HA and lesser collagen composition.					
36099110	5	81	from	collagen	788:795	arg1	SA					821:822	SA	821:822	SA	821:822	ECM composition of hyaluronic acid (HA) and collagen in the subglottic area (SA) was also evaluated.					
36099110	5	81	from	collagen	788:795	arg1	area					815:818	the subglottic area	800:818	the subglottic area (SA)	800:823	ECM composition of hyaluronic acid (HA) and collagen in the subglottic area (SA) was also evaluated.					
36099110	8	82	theme	HA	1143:1144	arg1	composition					1164:1174	more HA and less collagen composition	1138:1174	more HA and less collagen composition	1138:1174	Surgical groups had more HA and less collagen composition, causing a higher HA to collagen ratio in relation to the surgical extent.					
36099110	3	83	theme	FE	460:461	arg1	group					464:468	1) flap elevation (FE) group	441:468	1) flap elevation (FE) group	441:468	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	3	83	theme	FE	460:461	arg1	groups					400:405	three surgical groups	385:405	three surgical groups based on the extent of surgeries	385:438	MATERIALS AND METHODS Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group.					
36099110	4	84	theme	lamina	686:691	arg1	propria					693:699	the lamina propria	682:699	the lamina propria on postoperative days (PODs) 3, 7, and 21	682:741	We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21.					
36099110	11	85	dep	CONCLUSION	1454:1463	arg1	demonstrated					1476:1487	demonstrated	1476:1487	demonstrated that thyroid surgery exposing the thyroid and trachea induces an increase in the SA with a higher HA and lesser collagen composition	1476:1620	CONCLUSION This study demonstrated that thyroid surgery exposing the thyroid and trachea induces an increase in the SA with a higher HA and lesser collagen composition.					
35788394	6	0	theme	KEGG	941:944	arg1	pathways					946:953	several KEGG pathways	933:953	several KEGG pathways alterations	933:965	Predictive functional metagenomes showed that the maternal intake of SGS caused several KEGG pathways alterations with respect to the genetic information processing and metabolism.					
35788394	4	1	theme	significant	593:603	arg1	changes					605:611	significant changes	593:611	significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group)	593:745	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	2	2	theme	immune	336:341	arg1	activation					343:352	maternal immune activation	327:352	maternal immune activation	327:352	We reported that maternal diet of sulforaphane glucosinolate (SGS) could prevent behavioral abnormalities in offspring after maternal immune activation.					
35788394	1	3	theme	Mounting	119:126	arg1	evidence					128:135	Mounting evidence	119:135	Mounting evidence	119:135	Mounting evidence suggests the impact of maternal diet on the health of offspring.					
35788394	10	4	theme	dietary	1534:1540	arg1	intake					1542:1547	the dietary intake	1530:1547	the dietary intake of SGS during pregnancy and lactation	1530:1585	In conclusion, these data suggest that the dietary intake of SGS during pregnancy and lactation might produce long-lasting beneficial effects in adult offspring through the persistent modulation of gut microbiota.					
35788394	0	5	theme	Long-lasting	0:11	arg1	effects					24:30	Long-lasting beneficial effects	0:30	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.	0:117	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.					
35788394	6	6	theme	maternal	903:910	arg1	intake					912:917	the maternal intake	899:917	the maternal intake of SGS	899:924	Predictive functional metagenomes showed that the maternal intake of SGS caused several KEGG pathways alterations with respect to the genetic information processing and metabolism.					
35788394	4	7	theme	SGS-3W	691:696	arg1	offspring					680:688	3-week-old offspring	669:688	3-week-old offspring (SGS-3W group)	669:703	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	4	7	theme	SGS-3W	691:696	arg1	group					698:702	SGS-3W group	691:702	SGS-3W group	691:702	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	9	8	theme	sex	1431:1433	arg1	differences					1435:1445	sex differences	1431:1445	sex differences of gut microbiota composition in offspring	1431:1488	Moreover, there were sex differences of gut microbiota composition in offspring.					
35788394	6	9	theme	information	995:1005	arg1	processing					1007:1016	the genetic information processing	983:1016	the genetic information processing	983:1016	Predictive functional metagenomes showed that the maternal intake of SGS caused several KEGG pathways alterations with respect to the genetic information processing and metabolism.					
35788394	4	10	theme	gut	651:653	arg1	microbiota					655:664	gut microbiota	651:664	gut microbiota	651:664	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	7	11	theme	necrosis	1099:1106	arg1	TNF-α					1118:1122	TNF-α	1118:1122	TNF-α	1118:1122	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	7	11	theme	necrosis	1099:1106	arg1	factor-α					1108:1115	tumor necrosis factor-α	1093:1115	tumor necrosis factor-α (TNF-α)	1093:1123	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	10	12	theme	long-lasting	1601:1612	arg1	effects					1625:1631	long-lasting beneficial effects	1601:1631	long-lasting beneficial effects	1601:1631	In conclusion, these data suggest that the dietary intake of SGS during pregnancy and lactation might produce long-lasting beneficial effects in adult offspring through the persistent modulation of gut microbiota.					
35788394	11	13	theme	stress-related	1824:1837	arg1	disorders					1851:1859	stress-related psychiatric disorders	1824:1859	stress-related psychiatric disorders in the offspring	1824:1876	It is likely that the modulation of gut microbiota by maternal nutrition may confer resilience versus vulnerability to stress-related psychiatric disorders in the offspring.					
35788394	7	14	theme	plasma	1051:1056	arg1	lower					1224:1228	lower	1224:1228	lower	1224:1228	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	7	14	theme	plasma	1051:1056	arg1	levels					1058:1063	the plasma levels	1047:1063	the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg)	1047:1203	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	9	15	theme	microbiota	1454:1463	arg1	composition					1465:1475	gut microbiota composition	1450:1475	gut microbiota composition	1450:1475	Moreover, there were sex differences of gut microbiota composition in offspring.					
35788394	8	16	theme	positive	1296:1303	arg1	correlations					1305:1316	positive correlations	1296:1316	positive correlations between the relative abundance of the genus Blautia and IL-6 (or TNF-α) in adult offspring	1296:1407	It is noteworthy that there were positive correlations between the relative abundance of the genus Blautia and IL-6 (or TNF-α) in adult offspring.					
35788394	7	17	theme	interleukin-6	1068:1080	arg1	lower					1224:1228	lower	1224:1228	lower	1224:1228	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	7	17	theme	interleukin-6	1068:1080	arg1	levels					1058:1063	the plasma levels	1047:1063	the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg)	1047:1203	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	4	18	dep	α-diversity	620:630	arg1	the					616:618	the	616:618	the	616:618	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	0	19	theme	glucosinolate	67:79	arg1	intake					44:49	maternal intake	35:49	maternal intake of sulforaphane glucosinolate	35:79	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.					
35788394	5	20	from	phylotypes	809:818	arg1	groups					845:850	the SGS-3W or SGS-10W groups	823:850	the SGS-3W or SGS-10W groups	823:850	The LEfSe algorithm identified several microbes as important phylotypes in the SGS-3W or SGS-10W groups.					
35788394	2	21	theme	sulforaphane	236:247	arg1	glucosinolate					249:261	sulforaphane glucosinolate	236:261	sulforaphane glucosinolate (SGS)	236:267	We reported that maternal diet of sulforaphane glucosinolate (SGS) could prevent behavioral abnormalities in offspring after maternal immune activation.					
35788394	2	21	theme	sulforaphane	236:247	arg1	SGS					264:266	SGS	264:266	SGS	264:266	We reported that maternal diet of sulforaphane glucosinolate (SGS) could prevent behavioral abnormalities in offspring after maternal immune activation.					
35788394	0	22	from	effects	24:30	arg1	microbiota					88:97	gut microbiota	84:97	gut microbiota in adult offspring	84:116	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.					
35788394	7	23	dep	lipopolysaccharide	1169:1186	arg1	LPS					1189:1191	LPS	1189:1191	LPS: 0.5 mg/kg	1189:1202	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	4	24	theme	10-week-old	709:719	arg1	offspring					721:729	10-week-old offspring	709:729	10-week-old offspring (SGS-10W group)	709:745	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	4	24	theme	10-week-old	709:719	arg1	group					740:744	SGS-10W group	732:744	SGS-10W group	732:744	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	11	25	theme	maternal	1759:1766	arg1	nutrition					1768:1776	maternal nutrition	1759:1776	maternal nutrition	1759:1776	It is likely that the modulation of gut microbiota by maternal nutrition may confer resilience versus vulnerability to stress-related psychiatric disorders in the offspring.					
35788394	0	26	theme	gut	84:86	arg1	microbiota					88:97	gut microbiota	84:97	gut microbiota in adult offspring	84:116	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.					
35788394	1	27	theme	diet	169:172	arg1	impact					150:155	the impact	146:155	the impact of maternal diet on the health of offspring	146:199	Mounting evidence suggests the impact of maternal diet on the health of offspring.					
35788394	7	28	dep	LPS	1189:1191	arg1	mg/kg					1198:1202	0.5 mg/kg	1194:1202	LPS: 0.5 mg/kg	1189:1202	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	11	29	theme	microbiota	1745:1754	arg1	modulation					1727:1736	the modulation	1723:1736	the modulation of gut microbiota by maternal nutrition	1723:1776	It is likely that the modulation of gut microbiota by maternal nutrition may confer resilience versus vulnerability to stress-related psychiatric disorders in the offspring.					
35788394	4	30	from	changes	605:611	arg1	group					740:744	SGS-10W group	732:744	SGS-10W group	732:744	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	4	30	from	changes	605:611	arg1	group					698:702	SGS-3W group	691:702	SGS-3W group	691:702	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	4	30	from	changes	605:611	arg1	α-diversity					620:630	α-diversity	620:630	α-diversity	620:630	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	4	30	from	changes	605:611	arg1	β-diversity					636:646	β-diversity	636:646	β-diversity	636:646	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	4	30	from	changes	605:611	arg1	offspring					680:688	3-week-old offspring	669:688	3-week-old offspring (SGS-3W group)	669:703	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	4	30	from	changes	605:611	arg1	offspring					721:729	10-week-old offspring	709:729	10-week-old offspring (SGS-10W group)	709:745	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	1	31	from	impact	150:155	arg1	health					181:186	the health	177:186	the health of offspring	177:199	Mounting evidence suggests the impact of maternal diet on the health of offspring.					
35788394	8	32	theme	genus	1356:1360	arg1	Blautia					1362:1368	the genus Blautia	1352:1368	the genus Blautia	1352:1368	It is noteworthy that there were positive correlations between the relative abundance of the genus Blautia and IL-6 (or TNF-α) in adult offspring.					
35788394	5	33	theme	LEfSe	752:756	arg1	algorithm					758:766	The LEfSe algorithm	748:766	The LEfSe algorithm	748:766	The LEfSe algorithm identified several microbes as important phylotypes in the SGS-3W or SGS-10W groups.					
35788394	3	34	theme	SGS	431:433	arg1	intake					421:426	the dietary intake	409:426	the dietary intake of SGS during pregnancy and lactation	409:464	The present study was designed to investigate whether the dietary intake of SGS during pregnancy and lactation influences the composition of gut microbiota in the offspring.					
35788394	5	35	theme	SGS-3W	827:832	arg1	groups					845:850	the SGS-3W or SGS-10W groups	823:850	the SGS-3W or SGS-10W groups	823:850	The LEfSe algorithm identified several microbes as important phylotypes in the SGS-3W or SGS-10W groups.					
35788394	5	36	theme	SGS-10W	837:843	arg1	groups					845:850	the SGS-3W or SGS-10W groups	823:850	the SGS-3W or SGS-10W groups	823:850	The LEfSe algorithm identified several microbes as important phylotypes in the SGS-3W or SGS-10W groups.					
35788394	0	37	theme	maternal	35:42	arg1	intake					44:49	maternal intake	35:49	maternal intake of sulforaphane glucosinolate	35:79	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.					
35788394	5	38	theme	important	799:807	arg1	phylotypes					809:818	important phylotypes	799:818	important phylotypes in the SGS-3W or SGS-10W groups	799:850	The LEfSe algorithm identified several microbes as important phylotypes in the SGS-3W or SGS-10W groups.					
35788394	5	38	theme	important	799:807	arg1	microbes					787:794	several microbes	779:794	several microbes	779:794	The LEfSe algorithm identified several microbes as important phylotypes in the SGS-3W or SGS-10W groups.					
35788394	4	39	theme	dietary	533:539	arg1	intake					541:546	The dietary intake	529:546	The dietary intake of SGS during pregnancy and lactation	529:584	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	3	40	theme	microbiota	500:509	arg1	composition					481:491	the composition	477:491	the composition of gut microbiota in the offspring	477:526	The present study was designed to investigate whether the dietary intake of SGS during pregnancy and lactation influences the composition of gut microbiota in the offspring.					
35788394	6	41	theme	functional	864:873	arg1	metagenomes					875:885	Predictive functional metagenomes	853:885	Predictive functional metagenomes	853:885	Predictive functional metagenomes showed that the maternal intake of SGS caused several KEGG pathways alterations with respect to the genetic information processing and metabolism.					
35788394	9	42	from	differences	1435:1445	arg1	offspring					1480:1488	offspring	1480:1488	offspring	1480:1488	Moreover, there were sex differences of gut microbiota composition in offspring.					
35788394	3	43	from	composition	481:491	arg1	offspring					518:526	the offspring	514:526	the offspring	514:526	The present study was designed to investigate whether the dietary intake of SGS during pregnancy and lactation influences the composition of gut microbiota in the offspring.					
35788394	10	44	theme	gut	1689:1691	arg1	microbiota					1693:1702	gut microbiota	1689:1702	gut microbiota	1689:1702	In conclusion, these data suggest that the dietary intake of SGS during pregnancy and lactation might produce long-lasting beneficial effects in adult offspring through the persistent modulation of gut microbiota.					
35788394	8	45	from	abundance	1339:1347	arg1	offspring					1399:1407	adult offspring	1393:1407	adult offspring	1393:1407	It is noteworthy that there were positive correlations between the relative abundance of the genus Blautia and IL-6 (or TNF-α) in adult offspring.					
35788394	6	46	theme	pathways	946:953	arg1	alterations					955:965	several KEGG pathways alterations	933:965	several KEGG pathways alterations	933:965	Predictive functional metagenomes showed that the maternal intake of SGS caused several KEGG pathways alterations with respect to the genetic information processing and metabolism.					
35788394	7	47	theme	SGS-10W	1132:1138	arg1	group					1140:1144	the SGS-10W group	1128:1144	the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg)	1128:1203	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	8	48	from	IL-6	1374:1377	arg1	offspring					1399:1407	adult offspring	1393:1407	adult offspring	1393:1407	It is noteworthy that there were positive correlations between the relative abundance of the genus Blautia and IL-6 (or TNF-α) in adult offspring.					
35788394	6	49	theme	several	933:939	arg1	pathways					946:953	several KEGG pathways	933:953	several KEGG pathways alterations	933:965	Predictive functional metagenomes showed that the maternal intake of SGS caused several KEGG pathways alterations with respect to the genetic information processing and metabolism.					
35788394	10	50	theme	SGS	1552:1554	arg1	intake					1542:1547	the dietary intake	1530:1547	the dietary intake of SGS during pregnancy and lactation	1530:1585	In conclusion, these data suggest that the dietary intake of SGS during pregnancy and lactation might produce long-lasting beneficial effects in adult offspring through the persistent modulation of gut microbiota.					
35788394	2	51	theme	behavioral	283:292	arg1	abnormalities					294:306	behavioral abnormalities	283:306	behavioral abnormalities in offspring	283:319	We reported that maternal diet of sulforaphane glucosinolate (SGS) could prevent behavioral abnormalities in offspring after maternal immune activation.					
35788394	6	52	theme	SGS	922:924	arg1	intake					912:917	the maternal intake	899:917	the maternal intake of SGS	899:924	Predictive functional metagenomes showed that the maternal intake of SGS caused several KEGG pathways alterations with respect to the genetic information processing and metabolism.					
35788394	8	53	theme	relative	1330:1337	arg1	abundance					1339:1347	the relative abundance	1326:1347	the relative abundance of the genus Blautia	1326:1368	It is noteworthy that there were positive correlations between the relative abundance of the genus Blautia and IL-6 (or TNF-α) in adult offspring.					
35788394	4	54	theme	3-week-old	669:678	arg1	offspring					680:688	3-week-old offspring	669:688	3-week-old offspring (SGS-3W group)	669:703	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	4	54	theme	3-week-old	669:678	arg1	group					698:702	SGS-3W group	691:702	SGS-3W group	691:702	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	7	55	theme	factor-α	1108:1115	arg1	lower					1224:1228	lower	1224:1228	lower	1224:1228	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	7	55	theme	factor-α	1108:1115	arg1	levels					1058:1063	the plasma levels	1047:1063	the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg)	1047:1203	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	7	56	theme	tumor	1093:1097	arg1	TNF-α					1118:1122	TNF-α	1118:1122	TNF-α	1118:1122	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	7	56	theme	tumor	1093:1097	arg1	factor-α					1108:1115	tumor necrosis factor-α	1093:1115	tumor necrosis factor-α (TNF-α)	1093:1123	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	1	57	theme	offspring	191:199	arg1	health					181:186	the health	177:186	the health of offspring	177:199	Mounting evidence suggests the impact of maternal diet on the health of offspring.					
35788394	6	58	theme	genetic	987:993	arg1	information					995:1005	the genetic information	983:1005	the genetic information processing	983:1016	Predictive functional metagenomes showed that the maternal intake of SGS caused several KEGG pathways alterations with respect to the genetic information processing and metabolism.					
35788394	0	59	from	microbiota	88:97	arg1	offspring					108:116	adult offspring	102:116	adult offspring	102:116	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.					
35788394	10	60	theme	beneficial	1614:1623	arg1	effects					1625:1631	long-lasting beneficial effects	1601:1631	long-lasting beneficial effects	1601:1631	In conclusion, these data suggest that the dietary intake of SGS during pregnancy and lactation might produce long-lasting beneficial effects in adult offspring through the persistent modulation of gut microbiota.					
35788394	9	61	theme	gut	1450:1452	arg1	composition					1465:1475	gut microbiota composition	1450:1475	gut microbiota composition	1450:1475	Moreover, there were sex differences of gut microbiota composition in offspring.					
35788394	4	62	theme	microbiota	655:664	arg1	α-diversity					620:630	α-diversity	620:630	α-diversity	620:630	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	4	62	theme	microbiota	655:664	arg1	β-diversity					636:646	β-diversity	636:646	β-diversity	636:646	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	11	63	theme	psychiatric	1839:1849	arg1	disorders					1851:1859	stress-related psychiatric disorders	1824:1859	stress-related psychiatric disorders in the offspring	1824:1876	It is likely that the modulation of gut microbiota by maternal nutrition may confer resilience versus vulnerability to stress-related psychiatric disorders in the offspring.					
35788394	2	64	theme	maternal	219:226	arg1	diet					228:231	maternal diet	219:231	maternal diet of sulforaphane glucosinolate (SGS)	219:267	We reported that maternal diet of sulforaphane glucosinolate (SGS) could prevent behavioral abnormalities in offspring after maternal immune activation.					
35788394	9	65	theme	composition	1465:1475	arg1	differences					1435:1445	sex differences	1431:1445	sex differences of gut microbiota composition in offspring	1431:1488	Moreover, there were sex differences of gut microbiota composition in offspring.					
35788394	4	66	theme	SGS-10W	732:738	arg1	offspring					721:729	10-week-old offspring	709:729	10-week-old offspring (SGS-10W group)	709:745	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	4	66	theme	SGS-10W	732:738	arg1	group					740:744	SGS-10W group	732:744	SGS-10W group	732:744	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	11	67	theme	gut	1741:1743	arg1	microbiota					1745:1754	gut microbiota	1741:1754	gut microbiota	1741:1754	It is likely that the modulation of gut microbiota by maternal nutrition may confer resilience versus vulnerability to stress-related psychiatric disorders in the offspring.					
35788394	2	68	theme	glucosinolate	249:261	arg1	diet					228:231	maternal diet	219:231	maternal diet of sulforaphane glucosinolate (SGS)	219:267	We reported that maternal diet of sulforaphane glucosinolate (SGS) could prevent behavioral abnormalities in offspring after maternal immune activation.					
35788394	5	69	theme	several	779:785	arg1	phylotypes					809:818	important phylotypes	799:818	important phylotypes in the SGS-3W or SGS-10W groups	799:850	The LEfSe algorithm identified several microbes as important phylotypes in the SGS-3W or SGS-10W groups.					
35788394	5	69	theme	several	779:785	arg1	microbes					787:794	several microbes	779:794	several microbes	779:794	The LEfSe algorithm identified several microbes as important phylotypes in the SGS-3W or SGS-10W groups.					
35788394	1	70	theme	maternal	160:167	arg1	diet					169:172	maternal diet	160:172	maternal diet	160:172	Mounting evidence suggests the impact of maternal diet on the health of offspring.					
35788394	2	71	from	abnormalities	294:306	arg1	offspring					311:319	offspring	311:319	offspring	311:319	We reported that maternal diet of sulforaphane glucosinolate (SGS) could prevent behavioral abnormalities in offspring after maternal immune activation.					
35788394	0	72	theme	adult	102:106	arg1	offspring					108:116	adult offspring	102:116	adult offspring	102:116	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.					
35788394	2	73	theme	maternal	327:334	arg1	activation					343:352	maternal immune activation	327:352	maternal immune activation	327:352	We reported that maternal diet of sulforaphane glucosinolate (SGS) could prevent behavioral abnormalities in offspring after maternal immune activation.					
35788394	3	74	theme	present	359:365	arg1	study					367:371	The present study	355:371	The present study	355:371	The present study was designed to investigate whether the dietary intake of SGS during pregnancy and lactation influences the composition of gut microbiota in the offspring.					
35788394	11	75	from	disorders	1851:1859	arg1	offspring					1868:1876	the offspring	1864:1876	the offspring	1864:1876	It is likely that the modulation of gut microbiota by maternal nutrition may confer resilience versus vulnerability to stress-related psychiatric disorders in the offspring.					
35788394	10	76	theme	adult	1636:1640	arg1	offspring					1642:1650	adult offspring	1636:1650	adult offspring	1636:1650	In conclusion, these data suggest that the dietary intake of SGS during pregnancy and lactation might produce long-lasting beneficial effects in adult offspring through the persistent modulation of gut microbiota.					
35788394	0	77	theme	beneficial	13:22	arg1	effects					24:30	Long-lasting beneficial effects	0:30	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.	0:117	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.					
35788394	8	78	theme	Blautia	1362:1368	arg1	abundance					1339:1347	the relative abundance	1326:1347	the relative abundance of the genus Blautia	1326:1368	It is noteworthy that there were positive correlations between the relative abundance of the genus Blautia and IL-6 (or TNF-α) in adult offspring.					
35788394	8	78	theme	Blautia	1362:1368	arg1	IL-6					1374:1377	IL-6	1374:1377	IL-6 (or TNF-α) in adult offspring	1374:1407	It is noteworthy that there were positive correlations between the relative abundance of the genus Blautia and IL-6 (or TNF-α) in adult offspring.					
35788394	8	78	theme	Blautia	1362:1368	arg1	TNF-α					1383:1387	TNF-α	1383:1387	TNF-α	1383:1387	It is noteworthy that there were positive correlations between the relative abundance of the genus Blautia and IL-6 (or TNF-α) in adult offspring.					
35788394	7	79	theme	lipopolysaccharide	1169:1186	arg1	injection					1156:1164	the injection	1152:1164	the injection of lipopolysaccharide (LPS: 0.5 mg/kg)	1152:1203	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	10	80	theme	persistent	1664:1673	arg1	modulation					1675:1684	the persistent modulation	1660:1684	the persistent modulation of gut microbiota	1660:1702	In conclusion, these data suggest that the dietary intake of SGS during pregnancy and lactation might produce long-lasting beneficial effects in adult offspring through the persistent modulation of gut microbiota.					
35788394	0	81	theme	intake	44:49	arg1	effects					24:30	Long-lasting beneficial effects	0:30	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.	0:117	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.					
35788394	0	82	theme	sulforaphane	54:65	arg1	glucosinolate					67:79	sulforaphane glucosinolate	54:79	sulforaphane glucosinolate	54:79	Long-lasting beneficial effects of maternal intake of sulforaphane glucosinolate on gut microbiota in adult offspring.					
35788394	3	83	theme	dietary	413:419	arg1	intake					421:426	the dietary intake	409:426	the dietary intake of SGS during pregnancy and lactation	409:464	The present study was designed to investigate whether the dietary intake of SGS during pregnancy and lactation influences the composition of gut microbiota in the offspring.					
35788394	4	84	theme	SGS	551:553	arg1	intake					541:546	The dietary intake	529:546	The dietary intake of SGS during pregnancy and lactation	529:584	The dietary intake of SGS during pregnancy and lactation caused significant changes in the α-diversity and β-diversity of gut microbiota in 3-week-old offspring (SGS-3W group) and 10-week-old offspring (SGS-10W group).					
35788394	10	85	theme	microbiota	1693:1702	arg1	modulation					1675:1684	the persistent modulation	1660:1684	the persistent modulation of gut microbiota	1660:1702	In conclusion, these data suggest that the dietary intake of SGS during pregnancy and lactation might produce long-lasting beneficial effects in adult offspring through the persistent modulation of gut microbiota.					
35788394	6	86	theme	Predictive	853:862	arg1	metagenomes					875:885	Predictive functional metagenomes	853:885	Predictive functional metagenomes	853:885	Predictive functional metagenomes showed that the maternal intake of SGS caused several KEGG pathways alterations with respect to the genetic information processing and metabolism.					
35788394	8	87	theme	adult	1393:1397	arg1	offspring					1399:1407	adult offspring	1393:1407	adult offspring	1393:1407	It is noteworthy that there were positive correlations between the relative abundance of the genus Blautia and IL-6 (or TNF-α) in adult offspring.					
35788394	3	88	theme	gut	496:498	arg1	microbiota					500:509	gut microbiota	496:509	gut microbiota	496:509	The present study was designed to investigate whether the dietary intake of SGS during pregnancy and lactation influences the composition of gut microbiota in the offspring.					
35788394	7	89	from	levels	1058:1063	arg1	group					1140:1144	the SGS-10W group	1128:1144	the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg)	1128:1203	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35788394	7	90	theme	CON-10W	1248:1254	arg1	group					1256:1260	the CON-10W group	1244:1260	the CON-10W group	1244:1260	Furthermore, the plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the SGS-10W group after the injection of lipopolysaccharide (LPS: 0.5 mg/kg) were significantly lower than those of the CON-10W group.					
35616201	16	0	theme	UiO-66-NH2	2260:2269	arg1	UiO-66-NH2					2260:2269	UiO-66-NH2	2260:2269	UiO-66-NH2	2260:2269	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	16	0	theme	UiO-66-NH2	2260:2269	arg1	amount					2250:2255	the loading amount	2238:2255	the loading amount of UiO-66-NH2 in the hybrid aerogel	2238:2291	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	3	1	from	detection	367:375	arg1	products					401:408	health products	394:408	health products	394:408	Therefore, it is imperative to develop a method for the detection of sildenafil in health products.					
35616201	11	2	theme	carboxymethyl	1488:1500	arg1	CMC					1513:1515	CMC	1513:1515	CMC	1513:1515	Cellulose nanocrystal (CNC) was modified with an aldehyde group to obtain CNC-CHO, and carboxymethyl cellulose (CMC) was modified with a hydrazide group to obtain CMC-NHNH2.					
35616201	11	2	theme	carboxymethyl	1488:1500	arg1	cellulose					1502:1510	carboxymethyl cellulose	1488:1510	carboxymethyl cellulose (CMC)	1488:1516	Cellulose nanocrystal (CNC) was modified with an aldehyde group to obtain CNC-CHO, and carboxymethyl cellulose (CMC) was modified with a hydrazide group to obtain CMC-NHNH2.					
35616201	6	3	theme	metal	780:784	arg1	ions					786:789	metal ions	780:789	metal ions	780:789	Metal-organic frameworks (MOFs) are hybrid crystalline materials composed of metal ions and organic ligands.					
35616201	18	4	theme	0	2723:2723	arg1	min					2670:2672	40 min	2667:2672	40 min	2667:2672	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	4	theme	0	2723:2723	arg1	min					2626:2628	60 min	2623:2628	60 min	2623:2628	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	4	theme	0	2723:2723	arg1	mL					2695:2696	3×2 mL	2691:2696	3×2 mL	2691:2696	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	4	theme	0	2723:2723	arg1	acetonitrile					2639:2650	acetonitrile	2639:2650	acetonitrile	2639:2650	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	28	5	from	presence	4064:4071	arg1	others					4145:4150	the others	4141:4150	the others	4141:4150	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	28	5	from	presence	4064:4071	arg1	one					4100:4102	one	4100:4102	one	4100:4102	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	28	5	from	presence	4064:4071	arg1	products					4111:4118	the products	4107:4118	the products	4107:4118	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	18	6	theme	extraction	2606:2615	arg1	time					2617:2620	extraction time	2606:2620	extraction time	2606:2620	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	4	7	theme	matrix	548:553	arg1	interference					555:566	the matrix interference	544:566	the matrix interference of the samples	544:581	Solid-phase extraction (SPE) is typically used for the separation, purification, and enrichment of samples, with the aim of reducing the matrix interference of the samples and improving the detection sensitivity.					
35616201	26	8	theme	used	3704:3707	arg1	aerogels					3719:3726	The used composite aerogels	3700:3726	The used composite aerogels	3700:3726	The used composite aerogels were freeze-dried again and reused to extract sildenafil; the recovery was still maintained at 85.23% after five extraction cycles, indicating that the UiO-66-NH2@cellulose hybrid aerogel had good regeneration ability.					
35616201	11	9	theme	Cellulose	1401:1409	arg1	CNC					1424:1426	CNC	1424:1426	CNC	1424:1426	Cellulose nanocrystal (CNC) was modified with an aldehyde group to obtain CNC-CHO, and carboxymethyl cellulose (CMC) was modified with a hydrazide group to obtain CMC-NHNH2.					
35616201	11	9	theme	Cellulose	1401:1409	arg1	nanocrystal					1411:1421	Cellulose nanocrystal	1401:1421	Cellulose nanocrystal (CNC)	1401:1427	Cellulose nanocrystal (CNC) was modified with an aldehyde group to obtain CNC-CHO, and carboxymethyl cellulose (CMC) was modified with a hydrazide group to obtain CMC-NHNH2.					
35616201	15	10	theme	aerogel	2134:2140	arg1	surface					2112:2118	the pore surface	2103:2118	the pore surface of the hybrid aerogel, which made the pore of aerogel become more regular and a larger surface area	2103:2218	The results showed that UiO-66-NH2 was successfully loaded on the pore surface of the hybrid aerogel, which made the pore of aerogel become more regular and a larger surface area.					
35616201	12	11	theme	CNC-CHO	1617:1623	arg1	solution					1625:1632	the CNC-CHO solution	1613:1632	the CNC-CHO solution	1613:1632	Subsequently, UiO-66-NH2 was added to the CNC-CHO solution, mixed with the CMC-NHNH2 solution, and then crosslinked to load UiO-66-NH2 and form a hybrid aerogel as a bulk adsorbent.					
35616201	20	12	theme	detection	2940:2948	arg1	wavelength					2950:2959	The detection wavelength	2936:2959	The detection wavelength	2936:2959	The detection wavelength was set at 292 nm.					
35616201	8	13	theme	substrate	1133:1141	arg1	material					1143:1150	a substrate material	1131:1150	a substrate material	1131:1150	Owing to their advantages, researchers have attempted to use MOFs for SPE; however, it is difficult to collect MOFs in the powder form when they are directly used in SPE, necessitating the use of a substrate material to fix the MOFs.					
35616201	26	14	contain	had	3916:3918	arg1	aerogel					3908:3914	the UiO-66-NH2@cellulose hybrid aerogel	3876:3914	the UiO-66-NH2@cellulose hybrid aerogel	3876:3914	The used composite aerogels were freeze-dried again and reused to extract sildenafil; the recovery was still maintained at 85.23% after five extraction cycles, indicating that the UiO-66-NH2@cellulose hybrid aerogel had good regeneration ability.					
35616201	26	14	contain	had	3916:3918	arg2	ability					3938:3944	good regeneration ability	3920:3944	good regeneration ability	3920:3944	The used composite aerogels were freeze-dried again and reused to extract sildenafil; the recovery was still maintained at 85.23% after five extraction cycles, indicating that the UiO-66-NH2@cellulose hybrid aerogel had good regeneration ability.					
35616201	6	15	theme	crystalline	746:756	arg1	frameworks					717:726	Metal-organic frameworks	703:726	Metal-organic frameworks (MOFs)	703:733	Metal-organic frameworks (MOFs) are hybrid crystalline materials composed of metal ions and organic ligands.					
35616201	6	15	theme	crystalline	746:756	arg1	materials					758:766	hybrid crystalline materials	739:766	hybrid crystalline materials composed of metal ions and organic ligands	739:809	Metal-organic frameworks (MOFs) are hybrid crystalline materials composed of metal ions and organic ligands.					
35616201	11	16	mod	modified	1433:1440	arg3	group					1459:1463	an aldehyde group	1447:1463	an aldehyde group	1447:1463	Cellulose nanocrystal (CNC) was modified with an aldehyde group to obtain CNC-CHO, and carboxymethyl cellulose (CMC) was modified with a hydrazide group to obtain CMC-NHNH2.					
35616201	11	16	mod	modified	1433:1440	arg1	CNC					1424:1426	CNC	1424:1426	CNC	1424:1426	Cellulose nanocrystal (CNC) was modified with an aldehyde group to obtain CNC-CHO, and carboxymethyl cellulose (CMC) was modified with a hydrazide group to obtain CMC-NHNH2.					
35616201	11	16	mod	modified	1433:1440	arg1	nanocrystal					1411:1421	Cellulose nanocrystal	1401:1421	Cellulose nanocrystal (CNC)	1401:1427	Cellulose nanocrystal (CNC) was modified with an aldehyde group to obtain CNC-CHO, and carboxymethyl cellulose (CMC) was modified with a hydrazide group to obtain CMC-NHNH2.					
35616201	1	17	theme	pulmonary	162:170	arg1	hypertension					172:183	pulmonary hypertension	162:183	pulmonary hypertension	162:183	Sildenafil is a prescription drug used to treat pulmonary hypertension and erectile dysfunction.					
35616201	14	18	theme	nitrogen	2005:2012	arg1	adsorption/desorption					2018:2038	nitrogen gas adsorption/desorption	2005:2038	nitrogen gas adsorption/desorption	2005:2038	The synthesized hybrid aerogel was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform-infrared (FT-IR) spectroscopy, and nitrogen gas adsorption/desorption.					
35616201	9	19	with	method	1208:1213	arg1	sensitivity					1225:1235	high sensitivity	1220:1235	high sensitivity	1220:1235	Hence, this study proposes a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products.					
35616201	9	20	theme	health	1324:1329	arg1	products					1331:1338	health products	1324:1338	health products	1324:1338	Hence, this study proposes a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products.					
35616201	15	21	theme	larger	2200:2205	arg1	area					2215:2218	a larger surface area	2198:2218	a larger surface area	2198:2218	The results showed that UiO-66-NH2 was successfully loaded on the pore surface of the hybrid aerogel, which made the pore of aerogel become more regular and a larger surface area.					
35616201	16	22	from	amount	2250:2255	arg1	aerogel					2285:2291	the hybrid aerogel	2274:2291	the hybrid aerogel	2274:2291	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	8	23	used	used	1093:1096	arg2	they					1075:1078	they	1075:1078	they	1075:1078	Owing to their advantages, researchers have attempted to use MOFs for SPE; however, it is difficult to collect MOFs in the powder form when they are directly used in SPE, necessitating the use of a substrate material to fix the MOFs.					
35616201	22	24	theme	ng/mL	3213:3217	arg1	range					3196:3200	the range	3192:3200	the range of 10-2000 ng/mL	3192:3217	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	4	25	theme	detection	601:609	arg1	sensitivity					611:621	the detection sensitivity	597:621	the detection sensitivity	597:621	Solid-phase extraction (SPE) is typically used for the separation, purification, and enrichment of samples, with the aim of reducing the matrix interference of the samples and improving the detection sensitivity.					
35616201	28	26	theme	3.01	4076:4079	arg1	μg/g					4081:4084	μg/g	4081:4084	μg/g	4081:4084	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	5	27	theme	compounds	673:681	arg1	detection					654:662	the detection	650:662	the detection of trace compounds in complex samples	650:700	SPE is widely employed in the detection of trace compounds in complex samples.					
35616201	29	28	theme	89.12	4209:4213	arg1	%					4204:4204	%	4204:4204	%	4204:4204	The recoveries of this SPE method ranged from 74.93% to 89.12%, with RSDs in the range of 2.8%-5.3%, proving the feasibility of this analytical method.					
35616201	2	29	theme	illegal	224:230	arg1	hazardous					284:292	hazardous	284:292	hazardous	284:292	However, the illegal addition of sildenafil to health supplements may be hazardous to human health.					
35616201	2	29	theme	illegal	224:230	arg1	addition					232:239	the illegal addition	220:239	the illegal addition of sildenafil to health supplements	220:275	However, the illegal addition of sildenafil to health supplements may be hazardous to human health.					
35616201	29	30	theme	method	4180:4185	arg1	recoveries					4157:4166	The recoveries	4153:4166	The recoveries of this SPE method	4153:4185	The recoveries of this SPE method ranged from 74.93% to 89.12%, with RSDs in the range of 2.8%-5.3%, proving the feasibility of this analytical method.					
35616201	24	31	theme	previous	3360:3367	arg1	methods					3386:3392	previous preconcentration methods	3360:3392	previous preconcentration methods for sildenafil	3360:3407	Compared to previous preconcentration methods for sildenafil, this method offered a wider linear range.					
35616201	24	32	theme	linear	3438:3443	arg1	range					3445:3449	a wider linear range	3430:3449	a wider linear range	3430:3449	Compared to previous preconcentration methods for sildenafil, this method offered a wider linear range.					
35616201	18	33	theme	3×2	2691:2693	arg1	mL					2695:2696	3×2 mL	2691:2696	3×2 mL	2691:2696	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	33	theme	3×2	2691:2693	arg1	min					2626:2628	60 min	2623:2628	60 min	2623:2628	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	34	dep	pH	2583:2584	arg1	9.0					2601:2603	9.0	2601:2603	9.0	2601:2603	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	34	dep	pH	2583:2584	arg1	min					2626:2628	60 min	2623:2628	60 min	2623:2628	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	34	dep	pH	2583:2584	arg1	min					2670:2672	40 min	2667:2672	40 min	2667:2672	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	34	dep	pH	2583:2584	arg1	time					2617:2620	extraction time	2606:2620	extraction time	2606:2620	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	34	dep	pH	2583:2584	arg1	mL					2695:2696	3×2 mL	2691:2696	3×2 mL	2691:2696	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	34	dep	pH	2583:2584	arg1	acetonitrile					2639:2650	acetonitrile	2639:2650	acetonitrile	2639:2650	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	35	theme	sildenafil	2520:2529	arg1	enrichment					2506:2515	the enrichment	2502:2515	the enrichment of sildenafil	2502:2529	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	15	36	theme	aerogel	2166:2172	arg1	area					2215:2218	a larger surface area	2198:2218	a larger surface area	2198:2218	The results showed that UiO-66-NH2 was successfully loaded on the pore surface of the hybrid aerogel, which made the pore of aerogel become more regular and a larger surface area.					
35616201	15	36	theme	aerogel	2166:2172	arg1	pore					2158:2161	the pore	2154:2161	the pore of aerogel become more regular	2154:2192	The results showed that UiO-66-NH2 was successfully loaded on the pore surface of the hybrid aerogel, which made the pore of aerogel become more regular and a larger surface area.					
35616201	4	37	theme	Solid-phase	411:421	arg1	SPE					435:437	SPE	435:437	SPE	435:437	Solid-phase extraction (SPE) is typically used for the separation, purification, and enrichment of samples, with the aim of reducing the matrix interference of the samples and improving the detection sensitivity.					
35616201	4	37	theme	Solid-phase	411:421	arg1	extraction					423:432	Solid-phase extraction	411:432	Solid-phase extraction (SPE)	411:438	Solid-phase extraction (SPE) is typically used for the separation, purification, and enrichment of samples, with the aim of reducing the matrix interference of the samples and improving the detection sensitivity.					
35616201	17	38	theme	hybrid	2440:2445	arg1	aerogel					2447:2453	the hybrid aerogel	2436:2453	the hybrid aerogel	2436:2453	The highest amount of UiO-66-NH2 that could be loaded in the hybrid aerogel was 50%.					
35616201	27	39	theme	developed	3970:3978	arg1	method					3980:3985	the developed method	3966:3985	the developed method	3966:3985	The feasibility of the developed method was verified by analyzing five health products.					
35616201	19	40	theme	mol/L	2859:2863	arg1	30∶70					2903:2907	30∶70	2903:2907	30∶70	2903:2907	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	19	40	theme	mol/L	2859:2863	arg1	-acetonitrile					2888:2900	0.1 mol/L triethylamine (pH=6.50)-acetonitrile	2855:2900	0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v)	2855:2913	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	19	40	theme	mol/L	2859:2863	arg1	phase					2929:2933	the mobile phase	2918:2933	the mobile phase	2918:2933	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	0	41	theme	solid-phase	56:66	arg1	extraction					68:77	solid-phase extraction	56:77	solid-phase extraction of sildenafil	56:91	[Utilization of UiO-66-NH2@cellulose hybrid aerogel for solid-phase extraction of sildenafil in health products].					
35616201	22	42	theme	factor	3286:3291	arg1	limit					3225:3229	limit	3225:3229	limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17	3225:3300	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	7	43	theme	controllability	918:932	arg1	advantages					827:836	the advantages	823:836	the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability	823:932	MOFs offer the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability.					
35616201	25	44	dep	deviation	3593:3601	arg1	n=3					3609:3611	n=3	3609:3611	n=3	3609:3611	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	25	44	dep	deviation	3593:3601	arg1	RSD					3604:3606	RSD	3604:3606	RSD	3604:3606	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	15	45	theme	pore	2107:2110	arg1	surface					2112:2118	the pore surface	2103:2118	the pore surface of the hybrid aerogel, which made the pore of aerogel become more regular and a larger surface area	2103:2218	The results showed that UiO-66-NH2 was successfully loaded on the pore surface of the hybrid aerogel, which made the pore of aerogel become more regular and a larger surface area.					
35616201	14	46	theme	transform-infrared	1960:1977	arg1	spectroscopy					1987:1998	Fourier transform-infrared (FT-IR) spectroscopy	1952:1998	Fourier transform-infrared (FT-IR) spectroscopy	1952:1998	The synthesized hybrid aerogel was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform-infrared (FT-IR) spectroscopy, and nitrogen gas adsorption/desorption.					
35616201	24	47	theme	wider	3432:3436	arg1	range					3445:3449	a wider linear range	3430:3449	a wider linear range	3430:3449	Compared to previous preconcentration methods for sildenafil, this method offered a wider linear range.					
35616201	18	48	dep	following	2572:2580	arg1	pH					2583:2584	pH	2583:2584	pH of the sample	2583:2598	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	0	49	from	[Utilization	0:11	arg1	products					103:110	health products	96:110	health products	96:110	[Utilization of UiO-66-NH2@cellulose hybrid aerogel for solid-phase extraction of sildenafil in health products].					
35616201	16	50	from	Investigation	2221:2233	arg1	aerogel					2285:2291	the hybrid aerogel	2274:2291	the hybrid aerogel	2274:2291	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	26	51	theme	regeneration	3925:3936	arg1	ability					3938:3944	good regeneration ability	3920:3944	good regeneration ability	3920:3944	The used composite aerogels were freeze-dried again and reused to extract sildenafil; the recovery was still maintained at 85.23% after five extraction cycles, indicating that the UiO-66-NH2@cellulose hybrid aerogel had good regeneration ability.					
35616201	5	52	theme	complex	686:692	arg1	samples					694:700	complex samples	686:700	complex samples	686:700	SPE is widely employed in the detection of trace compounds in complex samples.					
35616201	7	53	theme	stability	898:906	arg1	advantages					827:836	the advantages	823:836	the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability	823:932	MOFs offer the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability.					
35616201	19	54	dep	Zorbax	2765:2770	arg1	Eclipse					2772:2778	Eclipse	2772:2778	Eclipse	2772:2778	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	28	55	from	sildenafil	4127:4136	arg1	others					4145:4150	the others	4141:4150	the others	4141:4150	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	28	55	from	sildenafil	4127:4136	arg1	one					4100:4102	one	4100:4102	one	4100:4102	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	28	55	from	sildenafil	4127:4136	arg1	products					4111:4118	the products	4107:4118	the products	4107:4118	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	14	56	theme	electron	1931:1938	arg1	microscopy					1940:1949	scanning electron microscopy	1922:1949	scanning electron microscopy	1922:1949	The synthesized hybrid aerogel was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform-infrared (FT-IR) spectroscopy, and nitrogen gas adsorption/desorption.					
35616201	17	57	theme	highest	2383:2389	arg1	UiO-66-NH2					2401:2410	UiO-66-NH2	2401:2410	UiO-66-NH2	2401:2410	The highest amount of UiO-66-NH2 that could be loaded in the hybrid aerogel was 50%.					
35616201	17	57	theme	highest	2383:2389	arg1	%					2461:2461	50%	2459:2461	50%	2459:2461	The highest amount of UiO-66-NH2 that could be loaded in the hybrid aerogel was 50%.					
35616201	17	57	theme	highest	2383:2389	arg1	amount					2391:2396	The highest amount	2379:2396	The highest amount of UiO-66-NH2 that could be loaded in the hybrid aerogel	2379:2453	The highest amount of UiO-66-NH2 that could be loaded in the hybrid aerogel was 50%.					
35616201	22	58	theme	sildenafil	3164:3173	arg1	concentration					3175:3187	the sildenafil concentration	3160:3187	the sildenafil concentration in the range of 10-2000 ng/mL	3160:3217	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	9	59	theme	novel	1198:1202	arg1	method					1208:1213	a novel SPE method	1196:1213	a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products	1196:1338	Hence, this study proposes a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products.					
35616201	12	60	theme	hybrid	1721:1726	arg1	aerogel					1728:1734	a hybrid aerogel	1719:1734	a hybrid aerogel	1719:1734	Subsequently, UiO-66-NH2 was added to the CNC-CHO solution, mixed with the CMC-NHNH2 solution, and then crosslinked to load UiO-66-NH2 and form a hybrid aerogel as a bulk adsorbent.					
35616201	12	60	theme	hybrid	1721:1726	arg1	bulk					1741:1744	a bulk	1739:1744	a bulk adsorbent	1739:1754	Subsequently, UiO-66-NH2 was added to the CNC-CHO solution, mixed with the CMC-NHNH2 solution, and then crosslinked to load UiO-66-NH2 and form a hybrid aerogel as a bulk adsorbent.					
35616201	25	61	theme	hybrid	3654:3659	arg1	aerogels					3661:3668	the prepared hybrid aerogels	3641:3668	the prepared hybrid aerogels	3641:3668	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	21	62	theme	cellulose	3025:3033	arg1	sildenafil					3086:3095	an adsorbent to extract sildenafil	3062:3095	an adsorbent to extract sildenafil in different concentrations	3062:3123	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	21	62	theme	cellulose	3025:3033	arg1	aerogel					3042:3048	the UiO-66-NH2@cellulose hybrid aerogel	3010:3048	the UiO-66-NH2@cellulose hybrid aerogel	3010:3048	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	26	63	theme	cellulose	3891:3899	arg1	aerogel					3908:3914	the UiO-66-NH2@cellulose hybrid aerogel	3876:3914	the UiO-66-NH2@cellulose hybrid aerogel	3876:3914	The used composite aerogels were freeze-dried again and reused to extract sildenafil; the recovery was still maintained at 85.23% after five extraction cycles, indicating that the UiO-66-NH2@cellulose hybrid aerogel had good regeneration ability.					
35616201	7	64	theme	permanent	872:880	arg1	nanopores					882:890	permanent nanopores	872:890	permanent nanopores	872:890	MOFs offer the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability.					
35616201	29	65	theme	analytical	4286:4295	arg1	method					4297:4302	this analytical method	4281:4302	this analytical method	4281:4302	The recoveries of this SPE method ranged from 74.93% to 89.12%, with RSDs in the range of 2.8%-5.3%, proving the feasibility of this analytical method.					
35616201	14	66	theme	powder	1902:1907	arg1	diffraction					1909:1919	X-ray powder diffraction	1896:1919	X-ray powder diffraction	1896:1919	The synthesized hybrid aerogel was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform-infrared (FT-IR) spectroscopy, and nitrogen gas adsorption/desorption.					
35616201	25	67	used	used	3543:3546	arg2	batches					3457:3463	Five batches	3452:3463	Five batches of hybrid aerogels	3452:3482	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	0	68	theme	UiO-66-NH2	16:25	arg1	aerogel					44:50	UiO-66-NH2@cellulose hybrid aerogel	16:50	UiO-66-NH2@cellulose hybrid aerogel	16:50	[Utilization of UiO-66-NH2@cellulose hybrid aerogel for solid-phase extraction of sildenafil in health products].					
35616201	9	69	theme	high	1220:1223	arg1	sensitivity					1225:1235	high sensitivity	1220:1235	high sensitivity	1220:1235	Hence, this study proposes a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products.					
35616201	9	70	from	detection	1297:1305	arg1	products					1331:1338	health products	1324:1338	health products	1324:1338	Hence, this study proposes a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products.					
35616201	28	71	theme	sildenafil	4086:4095	arg1	sildenafil					4127:4136	no sildenafil	4124:4136	no sildenafil in the others	4124:4150	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	28	71	theme	sildenafil	4086:4095	arg1	presence					4064:4071	the presence	4060:4071	the presence of 3.01 μg/g sildenafil in one of the products	4060:4118	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	16	72	from	aerogel	2285:2291	arg1	Investigation					2221:2233	Investigation	2221:2233	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel	2221:2291	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	21	73	theme	different	3100:3108	arg1	concentrations					3110:3123	different concentrations	3100:3123	different concentrations	3100:3123	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	18	74	theme	experimental	2468:2479	arg1	conditions					2481:2490	The experimental conditions	2464:2490	The experimental conditions affecting the enrichment of sildenafil	2464:2529	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	74	theme	experimental	2468:2479	arg1	following					2572:2580	the following	2568:2580	the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0	2568:2723	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	0	75	theme	cellulose	27:35	arg1	aerogel					44:50	UiO-66-NH2@cellulose hybrid aerogel	16:50	UiO-66-NH2@cellulose hybrid aerogel	16:50	[Utilization of UiO-66-NH2@cellulose hybrid aerogel for solid-phase extraction of sildenafil in health products].					
35616201	15	76	dep	pore	2158:2161	arg1	become					2174:2179	become	2174:2179	become more regular	2174:2192	The results showed that UiO-66-NH2 was successfully loaded on the pore surface of the hybrid aerogel, which made the pore of aerogel become more regular and a larger surface area.					
35616201	18	77	dep	min	2626:2628	arg1	eluent					2631:2636	eluent	2631:2636	eluent	2631:2636	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	77	dep	min	2626:2628	arg1	volume					2683:2688	elution volume	2675:2688	elution volume	2675:2688	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	77	dep	min	2626:2628	arg1	concentration					2708:2720	salt ion concentration	2699:2720	salt ion concentration	2699:2720	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	77	dep	min	2626:2628	arg1	time					2661:2664	elution time	2653:2664	elution time	2653:2664	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	11	78	theme	hydrazide	1538:1546	arg1	group					1548:1552	a hydrazide group	1536:1552	a hydrazide group	1536:1552	Cellulose nanocrystal (CNC) was modified with an aldehyde group to obtain CNC-CHO, and carboxymethyl cellulose (CMC) was modified with a hydrazide group to obtain CMC-NHNH2.					
35616201	26	79	theme	UiO-66-NH2	3880:3889	arg1	aerogel					3908:3914	the UiO-66-NH2@cellulose hybrid aerogel	3876:3914	the UiO-66-NH2@cellulose hybrid aerogel	3876:3914	The used composite aerogels were freeze-dried again and reused to extract sildenafil; the recovery was still maintained at 85.23% after five extraction cycles, indicating that the UiO-66-NH2@cellulose hybrid aerogel had good regeneration ability.					
35616201	4	80	used	used	453:456	arg2	SPE					435:437	SPE	435:437	SPE	435:437	Solid-phase extraction (SPE) is typically used for the separation, purification, and enrichment of samples, with the aim of reducing the matrix interference of the samples and improving the detection sensitivity.					
35616201	4	80	used	used	453:456	arg2	extraction					423:432	Solid-phase extraction	411:432	Solid-phase extraction (SPE)	411:438	Solid-phase extraction (SPE) is typically used for the separation, purification, and enrichment of samples, with the aim of reducing the matrix interference of the samples and improving the detection sensitivity.					
35616201	19	81	theme	C18	2785:2787	arg1	column					2789:2794	C18 column	2785:2794	C18 column (150 mm×4.6 mm, 5 μm)	2785:2816	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	16	82	theme	UiO-66-NH2	2326:2335	arg1	UiO-66-NH2					2326:2335	UiO-66-NH2	2326:2335	UiO-66-NH2	2326:2335	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	16	82	theme	UiO-66-NH2	2326:2335	arg1	amount					2316:2321	a higher amount	2307:2321	a higher amount of UiO-66-NH2	2307:2335	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	14	83	theme	hybrid	1860:1865	arg1	aerogel					1867:1873	The synthesized hybrid aerogel	1844:1873	The synthesized hybrid aerogel	1844:1873	The synthesized hybrid aerogel was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform-infrared (FT-IR) spectroscopy, and nitrogen gas adsorption/desorption.					
35616201	13	84	theme	bulk	1762:1765	arg1	aerogel					1774:1780	This bulk hybrid aerogel	1757:1780	This bulk hybrid aerogel	1757:1780	This bulk hybrid aerogel could be collected easily without extra force for use in SPE.					
35616201	6	85	theme	Metal-organic	703:715	arg1	frameworks					717:726	Metal-organic frameworks	703:726	Metal-organic frameworks (MOFs)	703:733	Metal-organic frameworks (MOFs) are hybrid crystalline materials composed of metal ions and organic ligands.					
35616201	6	85	theme	Metal-organic	703:715	arg1	materials					758:766	hybrid crystalline materials	739:766	hybrid crystalline materials composed of metal ions and organic ligands	739:809	Metal-organic frameworks (MOFs) are hybrid crystalline materials composed of metal ions and organic ligands.					
35616201	6	85	theme	Metal-organic	703:715	arg1	MOFs					729:732	MOFs	729:732	MOFs	729:732	Metal-organic frameworks (MOFs) are hybrid crystalline materials composed of metal ions and organic ligands.					
35616201	9	86	theme	@	1262:1262	arg1	aerogel					1280:1286	a UiO-66-NH2@cellulose hybrid aerogel	1250:1286	a UiO-66-NH2@cellulose hybrid aerogel	1250:1286	Hence, this study proposes a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products.					
35616201	16	87	theme	extraction	2356:2365	arg1	efficiency					2367:2376	better extraction efficiency	2349:2376	better extraction efficiency	2349:2376	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	29	88	theme	2.8	4243:4245	arg1	%					4246:4246	%	4246:4246	%	4246:4246	The recoveries of this SPE method ranged from 74.93% to 89.12%, with RSDs in the range of 2.8%-5.3%, proving the feasibility of this analytical method.					
35616201	29	89	from	RSDs	4222:4225	arg1	range					4234:4238	the range	4230:4238	the range of 2.8%-5.3%	4230:4251	The recoveries of this SPE method ranged from 74.93% to 89.12%, with RSDs in the range of 2.8%-5.3%, proving the feasibility of this analytical method.					
35616201	26	90	theme	extraction	3841:3850	arg1	cycles					3852:3857	five extraction cycles	3836:3857	five extraction cycles	3836:3857	The used composite aerogels were freeze-dried again and reused to extract sildenafil; the recovery was still maintained at 85.23% after five extraction cycles, indicating that the UiO-66-NH2@cellulose hybrid aerogel had good regeneration ability.					
35616201	29	91	theme	-5.3	4247:4250	arg1	%					4246:4246	%	4246:4246	%	4246:4246	The recoveries of this SPE method ranged from 74.93% to 89.12%, with RSDs in the range of 2.8%-5.3%, proving the feasibility of this analytical method.					
35616201	28	92	theme	μg/g	4081:4084	arg1	sildenafil					4086:4095	3.01 μg/g sildenafil	4076:4095	3.01 μg/g sildenafil	4076:4095	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	9	93	theme	sildenafil	1310:1319	arg1	detection					1297:1305	the detection	1293:1305	the detection of sildenafil in health products	1293:1338	Hence, this study proposes a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products.					
35616201	9	94	theme	hybrid	1273:1278	arg1	aerogel					1280:1286	a UiO-66-NH2@cellulose hybrid aerogel	1250:1286	a UiO-66-NH2@cellulose hybrid aerogel	1250:1286	Hence, this study proposes a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products.					
35616201	21	95	theme	optimal	2990:2996	arg1	conditions					2998:3007	the optimal conditions	2986:3007	the optimal conditions	2986:3007	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	21	96	used	used	3054:3057	arg2	aerogel					3042:3048	the UiO-66-NH2@cellulose hybrid aerogel	3010:3048	the UiO-66-NH2@cellulose hybrid aerogel	3010:3048	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	21	96	used	used	3054:3057	arg2	sildenafil					3086:3095	an adsorbent to extract sildenafil	3062:3095	an adsorbent to extract sildenafil in different concentrations	3062:3123	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	16	97	theme	amount	2250:2255	arg1	Investigation					2221:2233	Investigation	2221:2233	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel	2221:2291	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	7	98	theme	large	843:847	arg1	area					866:869	a large specific surface area	841:869	a large specific surface area	841:869	MOFs offer the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability.					
35616201	8	99	theme	powder	1058:1063	arg1	form					1065:1068	the powder form	1054:1068	the powder form	1054:1068	Owing to their advantages, researchers have attempted to use MOFs for SPE; however, it is difficult to collect MOFs in the powder form when they are directly used in SPE, necessitating the use of a substrate material to fix the MOFs.					
35616201	16	100	theme	hybrid	2278:2283	arg1	aerogel					2285:2291	the hybrid aerogel	2274:2291	the hybrid aerogel	2274:2291	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	26	101	theme	composite	3709:3717	arg1	aerogels					3719:3726	The used composite aerogels	3700:3726	The used composite aerogels	3700:3726	The used composite aerogels were freeze-dried again and reused to extract sildenafil; the recovery was still maintained at 85.23% after five extraction cycles, indicating that the UiO-66-NH2@cellulose hybrid aerogel had good regeneration ability.					
35616201	13	102	theme	hybrid	1767:1772	arg1	aerogel					1774:1780	This bulk hybrid aerogel	1757:1780	This bulk hybrid aerogel	1757:1780	This bulk hybrid aerogel could be collected easily without extra force for use in SPE.					
35616201	27	103	theme	method	3980:3985	arg1	feasibility					3951:3961	The feasibility	3947:3961	The feasibility of the developed method	3947:3985	The feasibility of the developed method was verified by analyzing five health products.					
35616201	5	104	from	detection	654:662	arg1	samples					694:700	complex samples	686:700	complex samples	686:700	SPE is widely employed in the detection of trace compounds in complex samples.					
35616201	25	105	theme	relative	3575:3582	arg1	deviation					3593:3601	the relative standard deviation	3571:3601	the relative standard deviation (RSD, n=3)	3571:3612	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	25	105	theme	relative	3575:3582	arg1	%					3622:3622	1.71%	3618:3622	1.71%	3618:3622	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	27	106	theme	health	4018:4023	arg1	products					4025:4032	five health products	4013:4032	five health products	4013:4032	The feasibility of the developed method was verified by analyzing five health products.					
35616201	22	107	from	concentration	3175:3187	arg1	range					3196:3200	the range	3192:3200	the range of 10-2000 ng/mL	3192:3217	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	26	108	theme	good	3920:3923	arg1	ability					3938:3944	good regeneration ability	3920:3944	good regeneration ability	3920:3944	The used composite aerogels were freeze-dried again and reused to extract sildenafil; the recovery was still maintained at 85.23% after five extraction cycles, indicating that the UiO-66-NH2@cellulose hybrid aerogel had good regeneration ability.					
35616201	0	109	theme	aerogel	44:50	arg1	[Utilization					0:11	[Utilization	0:11	[Utilization of UiO-66-NH2@cellulose hybrid aerogel for solid-phase extraction of sildenafil in health products	0:110	[Utilization of UiO-66-NH2@cellulose hybrid aerogel for solid-phase extraction of sildenafil in health products].					
35616201	19	110	dep	column	2789:2794	arg1	mm					2808:2809	150 mm×4.6 mm	2797:2809	150 mm×4.6 mm	2797:2809	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	19	110	dep	column	2789:2794	arg1	μm					2814:2815	5 μm	2812:2815	5 μm	2812:2815	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	25	111	theme	same	3523:3526	arg1	conditions					3528:3537	the same conditions	3519:3537	the same conditions	3519:3537	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	14	112	theme	gas	2014:2016	arg1	adsorption/desorption					2018:2038	nitrogen gas adsorption/desorption	2005:2038	nitrogen gas adsorption/desorption	2005:2038	The synthesized hybrid aerogel was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform-infrared (FT-IR) spectroscopy, and nitrogen gas adsorption/desorption.					
35616201	6	113	theme	hybrid	739:744	arg1	frameworks					717:726	Metal-organic frameworks	703:726	Metal-organic frameworks (MOFs)	703:733	Metal-organic frameworks (MOFs) are hybrid crystalline materials composed of metal ions and organic ligands.					
35616201	6	113	theme	hybrid	739:744	arg1	materials					758:766	hybrid crystalline materials	739:766	hybrid crystalline materials composed of metal ions and organic ligands	739:809	Metal-organic frameworks (MOFs) are hybrid crystalline materials composed of metal ions and organic ligands.					
35616201	8	114	theme	material	1143:1150	arg1	use					1124:1126	the use	1120:1126	the use of a substrate material	1120:1150	Owing to their advantages, researchers have attempted to use MOFs for SPE; however, it is difficult to collect MOFs in the powder form when they are directly used in SPE, necessitating the use of a substrate material to fix the MOFs.					
35616201	23	115	theme	correlation	3307:3317	arg1	0.9950					3340:3345	0.9950	3340:3345	0.9950	3340:3345	The correlation coefficient (R2) was 0.9950.					
35616201	23	115	theme	correlation	3307:3317	arg1	coefficient					3319:3329	The correlation coefficient	3303:3329	The correlation coefficient (R2)	3303:3334	The correlation coefficient (R2) was 0.9950.					
35616201	23	115	theme	correlation	3307:3317	arg1	R2					3332:3333	R2	3332:3333	R2	3332:3333	The correlation coefficient (R2) was 0.9950.					
35616201	29	116	dep	%	4204:4204	arg1	to					4206:4207	to	4206:4207	to	4206:4207	The recoveries of this SPE method ranged from 74.93% to 89.12%, with RSDs in the range of 2.8%-5.3%, proving the feasibility of this analytical method.					
35616201	19	117	theme	phosphate	2825:2833	arg1	solution					2835:2842	a phosphate solution	2823:2842	a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase	2823:2933	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	2	118	theme	human	297:301	arg1	health					303:308	human health	297:308	human health	297:308	However, the illegal addition of sildenafil to health supplements may be hazardous to human health.					
35616201	1	119	theme	erectile	189:196	arg1	dysfunction					198:208	erectile dysfunction	189:208	erectile dysfunction	189:208	Sildenafil is a prescription drug used to treat pulmonary hypertension and erectile dysfunction.					
35616201	25	120	theme	standard	3584:3591	arg1	deviation					3593:3601	the relative standard deviation	3571:3601	the relative standard deviation (RSD, n=3)	3571:3612	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	25	120	theme	standard	3584:3591	arg1	%					3622:3622	1.71%	3618:3622	1.71%	3618:3622	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	29	121	theme	SPE	4176:4178	arg1	method					4180:4185	this SPE method	4171:4185	this SPE method	4171:4185	The recoveries of this SPE method ranged from 74.93% to 89.12%, with RSDs in the range of 2.8%-5.3%, proving the feasibility of this analytical method.					
35616201	26	122	theme	extract	3766:3772	arg1	sildenafil					3774:3783	extract sildenafil	3766:3783	extract sildenafil	3766:3783	The used composite aerogels were freeze-dried again and reused to extract sildenafil; the recovery was still maintained at 85.23% after five extraction cycles, indicating that the UiO-66-NH2@cellulose hybrid aerogel had good regeneration ability.					
35616201	5	123	theme	trace	667:671	arg1	compounds					673:681	trace compounds	667:681	trace compounds	667:681	SPE is widely employed in the detection of trace compounds in complex samples.					
35616201	15	124	theme	surface	2207:2213	arg1	area					2215:2218	a larger surface area	2198:2218	a larger surface area	2198:2218	The results showed that UiO-66-NH2 was successfully loaded on the pore surface of the hybrid aerogel, which made the pore of aerogel become more regular and a larger surface area.					
35616201	13	125	from	force	1822:1826	arg1	SPE					1839:1841	SPE	1839:1841	SPE	1839:1841	This bulk hybrid aerogel could be collected easily without extra force for use in SPE.					
35616201	28	126	theme	products	4111:4118	arg1	one					4100:4102	one	4100:4102	one	4100:4102	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	28	126	theme	products	4111:4118	arg1	products					4111:4118	the products	4107:4118	the products	4107:4118	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	19	127	dep	30∶70	2903:2907	arg1	v/v					2910:2912	v/v	2910:2912	v/v	2910:2912	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	4	128	dep	separation	466:475	arg1	the					462:464	the	462:464	the	462:464	Solid-phase extraction (SPE) is typically used for the separation, purification, and enrichment of samples, with the aim of reducing the matrix interference of the samples and improving the detection sensitivity.					
35616201	14	129	theme	FT-IR	1980:1984	arg1	spectroscopy					1987:1998	Fourier transform-infrared (FT-IR) spectroscopy	1952:1998	Fourier transform-infrared (FT-IR) spectroscopy	1952:1998	The synthesized hybrid aerogel was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform-infrared (FT-IR) spectroscopy, and nitrogen gas adsorption/desorption.					
35616201	22	130	dep	detection	3234:3242	arg1	S/N=3					3250:3254	S/N=3	3250:3254	S/N=3	3250:3254	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	22	130	dep	detection	3234:3242	arg1	LOD					3245:3247	LOD	3245:3247	LOD	3245:3247	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	22	131	theme	detection	3234:3242	arg1	limit					3225:3229	limit	3225:3229	limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17	3225:3300	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	21	132	from	sildenafil	3086:3095	arg1	concentrations					3110:3123	different concentrations	3100:3123	different concentrations	3100:3123	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	10	133	theme	hydrothermal	1380:1391	arg1	method					1393:1398	a hydrothermal method	1378:1398	a hydrothermal method	1378:1398	First, UiO-66-NH2 was synthesized by a hydrothermal method.					
35616201	25	134	theme	good	3678:3681	arg1	reproducibility					3683:3697	good reproducibility	3678:3697	good reproducibility	3678:3697	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	19	135	theme	triethylamine	2865:2877	arg1	30∶70					2903:2907	30∶70	2903:2907	30∶70	2903:2907	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	19	135	theme	triethylamine	2865:2877	arg1	-acetonitrile					2888:2900	0.1 mol/L triethylamine (pH=6.50)-acetonitrile	2855:2900	0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v)	2855:2913	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	19	135	theme	triethylamine	2865:2877	arg1	phase					2929:2933	the mobile phase	2918:2933	the mobile phase	2918:2933	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	2	136	theme	health	258:263	arg1	supplements					265:275	health supplements	258:275	health supplements	258:275	However, the illegal addition of sildenafil to health supplements may be hazardous to human health.					
35616201	11	137	theme	aldehyde	1450:1457	arg1	group					1459:1463	an aldehyde group	1447:1463	an aldehyde group	1447:1463	Cellulose nanocrystal (CNC) was modified with an aldehyde group to obtain CNC-CHO, and carboxymethyl cellulose (CMC) was modified with a hydrazide group to obtain CMC-NHNH2.					
35616201	29	138	theme	74.93	4199:4203	arg1	%					4204:4204	%	4204:4204	%	4204:4204	The recoveries of this SPE method ranged from 74.93% to 89.12%, with RSDs in the range of 2.8%-5.3%, proving the feasibility of this analytical method.					
35616201	19	139	theme	0.1	2855:2857	arg1	mol/L					2859:2863	mol/L	2859:2863	mol/L	2859:2863	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	18	140	theme	elution	2675:2681	arg1	volume					2683:2688	elution volume	2675:2688	elution volume	2675:2688	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	2	141	theme	sildenafil	244:253	arg1	hazardous					284:292	hazardous	284:292	hazardous	284:292	However, the illegal addition of sildenafil to health supplements may be hazardous to human health.					
35616201	2	141	theme	sildenafil	244:253	arg1	addition					232:239	the illegal addition	220:239	the illegal addition of sildenafil to health supplements	220:275	However, the illegal addition of sildenafil to health supplements may be hazardous to human health.					
35616201	1	142	theme	prescription	130:141	arg1	drug					143:146	a prescription drug	128:146	a prescription drug used to treat pulmonary hypertension and erectile dysfunction	128:208	Sildenafil is a prescription drug used to treat pulmonary hypertension and erectile dysfunction.					
35616201	1	142	theme	prescription	130:141	arg1	Sildenafil					114:123	Sildenafil	114:123	Sildenafil	114:123	Sildenafil is a prescription drug used to treat pulmonary hypertension and erectile dysfunction.					
35616201	19	143	contain	containing	2844:2853	arg2	phase					2929:2933	the mobile phase	2918:2933	the mobile phase	2918:2933	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	19	143	contain	containing	2844:2853	arg2	30∶70					2903:2907	30∶70	2903:2907	30∶70	2903:2907	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	19	143	contain	containing	2844:2853	arg2	-acetonitrile					2888:2900	0.1 mol/L triethylamine (pH=6.50)-acetonitrile	2855:2900	0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v)	2855:2913	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	19	143	contain	containing	2844:2853	arg1	solution					2835:2842	a phosphate solution	2823:2842	a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase	2823:2933	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	18	144	theme	ion	2704:2706	arg1	concentration					2708:2720	salt ion concentration	2699:2720	salt ion concentration	2699:2720	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	0	145	theme	sildenafil	82:91	arg1	extraction					68:77	solid-phase extraction	56:77	solid-phase extraction of sildenafil	56:91	[Utilization of UiO-66-NH2@cellulose hybrid aerogel for solid-phase extraction of sildenafil in health products].					
35616201	22	146	theme	enrichment	3275:3284	arg1	factor					3286:3291	enrichment factor	3275:3291	enrichment factor	3275:3291	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	12	147	theme	adsorbent	1746:1754	arg1	aerogel					1728:1734	a hybrid aerogel	1719:1734	a hybrid aerogel	1719:1734	Subsequently, UiO-66-NH2 was added to the CNC-CHO solution, mixed with the CMC-NHNH2 solution, and then crosslinked to load UiO-66-NH2 and form a hybrid aerogel as a bulk adsorbent.					
35616201	12	147	theme	adsorbent	1746:1754	arg1	bulk					1741:1744	a bulk	1739:1744	a bulk adsorbent	1739:1754	Subsequently, UiO-66-NH2 was added to the CNC-CHO solution, mixed with the CMC-NHNH2 solution, and then crosslinked to load UiO-66-NH2 and form a hybrid aerogel as a bulk adsorbent.					
35616201	19	148	theme	Agilent	2757:2763	arg1	Zorbax					2765:2770	an Agilent Zorbax Eclipse	2754:2778	an Agilent Zorbax Eclipse	2754:2778	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	8	149	from	MOFs	1046:1049	arg1	form					1065:1068	the powder form	1054:1068	the powder form	1054:1068	Owing to their advantages, researchers have attempted to use MOFs for SPE; however, it is difficult to collect MOFs in the powder form when they are directly used in SPE, necessitating the use of a substrate material to fix the MOFs.					
35616201	14	150	theme	Fourier	1952:1958	arg1	spectroscopy					1987:1998	Fourier transform-infrared (FT-IR) spectroscopy	1952:1998	Fourier transform-infrared (FT-IR) spectroscopy	1952:1998	The synthesized hybrid aerogel was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform-infrared (FT-IR) spectroscopy, and nitrogen gas adsorption/desorption.					
35616201	25	151	theme	hybrid	3468:3473	arg1	aerogels					3475:3482	hybrid aerogels	3468:3482	hybrid aerogels	3468:3482	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	0	152	theme	health	96:101	arg1	products					103:110	health products	96:110	health products	96:110	[Utilization of UiO-66-NH2@cellulose hybrid aerogel for solid-phase extraction of sildenafil in health products].					
35616201	22	153	theme	ng/mL	3265:3269	arg1	detection					3234:3242	detection	3234:3242	detection (LOD, S/N=3) of 2.85 ng/mL	3234:3269	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	22	153	theme	ng/mL	3265:3269	arg1	factor					3286:3291	enrichment factor	3275:3291	enrichment factor	3275:3291	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	7	154	theme	pore	913:916	arg1	controllability					918:932	pore controllability	913:932	pore controllability	913:932	MOFs offer the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability.					
35616201	22	155	with	proportional	3144:3155	arg1	limit					3225:3229	limit	3225:3229	limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17	3225:3300	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	16	156	theme	loading	2242:2248	arg1	UiO-66-NH2					2260:2269	UiO-66-NH2	2260:2269	UiO-66-NH2	2260:2269	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	16	156	theme	loading	2242:2248	arg1	amount					2250:2255	the loading amount	2238:2255	the loading amount of UiO-66-NH2 in the hybrid aerogel	2238:2291	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	11	157	mod	modified	1522:1529	arg3	group					1548:1552	a hydrazide group	1536:1552	a hydrazide group	1536:1552	Cellulose nanocrystal (CNC) was modified with an aldehyde group to obtain CNC-CHO, and carboxymethyl cellulose (CMC) was modified with a hydrazide group to obtain CMC-NHNH2.					
35616201	11	157	mod	modified	1522:1529	arg1	CMC					1513:1515	CMC	1513:1515	CMC	1513:1515	Cellulose nanocrystal (CNC) was modified with an aldehyde group to obtain CNC-CHO, and carboxymethyl cellulose (CMC) was modified with a hydrazide group to obtain CMC-NHNH2.					
35616201	11	157	mod	modified	1522:1529	arg1	cellulose					1502:1510	carboxymethyl cellulose	1488:1510	carboxymethyl cellulose (CMC)	1488:1516	Cellulose nanocrystal (CNC) was modified with an aldehyde group to obtain CNC-CHO, and carboxymethyl cellulose (CMC) was modified with a hydrazide group to obtain CMC-NHNH2.					
35616201	18	158	theme	sample	2593:2598	arg1	pH					2583:2584	pH	2583:2584	pH of the sample	2583:2598	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	18	159	theme	elution	2653:2659	arg1	time					2661:2664	elution time	2653:2664	elution time	2653:2664	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	25	160	theme	prepared	3645:3652	arg1	aerogels					3661:3668	the prepared hybrid aerogels	3641:3668	the prepared hybrid aerogels	3641:3668	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	14	161	theme	scanning	1922:1929	arg1	microscopy					1940:1949	scanning electron microscopy	1922:1949	scanning electron microscopy	1922:1949	The synthesized hybrid aerogel was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform-infrared (FT-IR) spectroscopy, and nitrogen gas adsorption/desorption.					
35616201	18	162	theme	salt	2699:2702	arg1	concentration					2708:2720	salt ion concentration	2699:2720	salt ion concentration	2699:2720	The experimental conditions affecting the enrichment of sildenafil were optimized, and determined to be the following: pH of the sample, 9.0; extraction time, 60 min; eluent, acetonitrile; elution time, 40 min; elution volume, 3×2 mL; salt ion concentration, 0.					
35616201	9	163	theme	SPE	1204:1206	arg1	method					1208:1213	a novel SPE method	1196:1213	a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products	1196:1338	Hence, this study proposes a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products.					
35616201	7	164	theme	good	893:896	arg1	stability					898:906	good stability	893:906	good stability	893:906	MOFs offer the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability.					
35616201	25	165	theme	aerogels	3475:3482	arg1	batches					3457:3463	Five batches	3452:3463	Five batches of hybrid aerogels	3452:3482	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	21	166	theme	hybrid	3035:3040	arg1	sildenafil					3086:3095	an adsorbent to extract sildenafil	3062:3095	an adsorbent to extract sildenafil in different concentrations	3062:3123	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	21	166	theme	hybrid	3035:3040	arg1	aerogel					3042:3048	the UiO-66-NH2@cellulose hybrid aerogel	3010:3048	the UiO-66-NH2@cellulose hybrid aerogel	3010:3048	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	17	167	theme	UiO-66-NH2	2401:2410	arg1	UiO-66-NH2					2401:2410	UiO-66-NH2	2401:2410	UiO-66-NH2	2401:2410	The highest amount of UiO-66-NH2 that could be loaded in the hybrid aerogel was 50%.					
35616201	17	167	theme	UiO-66-NH2	2401:2410	arg1	%					2461:2461	50%	2459:2461	50%	2459:2461	The highest amount of UiO-66-NH2 that could be loaded in the hybrid aerogel was 50%.					
35616201	17	167	theme	UiO-66-NH2	2401:2410	arg1	amount					2391:2396	The highest amount	2379:2396	The highest amount of UiO-66-NH2 that could be loaded in the hybrid aerogel	2379:2453	The highest amount of UiO-66-NH2 that could be loaded in the hybrid aerogel was 50%.					
35616201	21	168	theme	UiO-66-NH2	3014:3023	arg1	sildenafil					3086:3095	an adsorbent to extract sildenafil	3062:3095	an adsorbent to extract sildenafil in different concentrations	3062:3123	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	21	168	theme	UiO-66-NH2	3014:3023	arg1	aerogel					3042:3048	the UiO-66-NH2@cellulose hybrid aerogel	3010:3048	the UiO-66-NH2@cellulose hybrid aerogel	3010:3048	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	19	169	theme	mobile	2922:2927	arg1	-acetonitrile					2888:2900	0.1 mol/L triethylamine (pH=6.50)-acetonitrile	2855:2900	0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v)	2855:2913	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	19	169	theme	mobile	2922:2927	arg1	phase					2929:2933	the mobile phase	2918:2933	the mobile phase	2918:2933	Separation was performed on an Agilent Zorbax Eclipse Plus C18 column (150 mm×4.6 mm, 5 μm) with a phosphate solution containing 0.1 mol/L triethylamine (pH=6.50)-acetonitrile (30∶70, v/v) as the mobile phase.					
35616201	0	170	theme	@	26:26	arg1	aerogel					44:50	UiO-66-NH2@cellulose hybrid aerogel	16:50	UiO-66-NH2@cellulose hybrid aerogel	16:50	[Utilization of UiO-66-NH2@cellulose hybrid aerogel for solid-phase extraction of sildenafil in health products].					
35616201	7	171	theme	nanopores	882:890	arg1	advantages					827:836	the advantages	823:836	the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability	823:932	MOFs offer the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability.					
35616201	29	172	theme	method	4297:4302	arg1	feasibility					4266:4276	the feasibility	4262:4276	the feasibility of this analytical method	4262:4302	The recoveries of this SPE method ranged from 74.93% to 89.12%, with RSDs in the range of 2.8%-5.3%, proving the feasibility of this analytical method.					
35616201	14	173	theme	X-ray	1896:1900	arg1	diffraction					1909:1919	X-ray powder diffraction	1896:1919	X-ray powder diffraction	1896:1919	The synthesized hybrid aerogel was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform-infrared (FT-IR) spectroscopy, and nitrogen gas adsorption/desorption.					
35616201	0	174	theme	hybrid	37:42	arg1	aerogel					44:50	UiO-66-NH2@cellulose hybrid aerogel	16:50	UiO-66-NH2@cellulose hybrid aerogel	16:50	[Utilization of UiO-66-NH2@cellulose hybrid aerogel for solid-phase extraction of sildenafil in health products].					
35616201	16	175	theme	higher	2309:2314	arg1	UiO-66-NH2					2326:2335	UiO-66-NH2	2326:2335	UiO-66-NH2	2326:2335	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	16	175	theme	higher	2309:2314	arg1	amount					2316:2321	a higher amount	2307:2321	a higher amount of UiO-66-NH2	2307:2335	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	7	176	theme	surface	858:864	arg1	area					866:869	a large specific surface area	841:869	a large specific surface area	841:869	MOFs offer the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability.					
35616201	3	177	theme	health	394:399	arg1	products					401:408	health products	394:408	health products	394:408	Therefore, it is imperative to develop a method for the detection of sildenafil in health products.					
35616201	28	178	attach	presence	4064:4071	arg1	others					4145:4150	the others	4141:4150	the others	4141:4150	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	28	178	attach	presence	4064:4071	arg1	products					4111:4118	the products	4107:4118	the products	4107:4118	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	28	178	attach	presence	4064:4071	arg1	one					4100:4102	one	4100:4102	one	4100:4102	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	28	178	attach	presence	4064:4071	arg2	sildenafil					4086:4095	3.01 μg/g sildenafil	4076:4095	3.01 μg/g sildenafil	4076:4095	The results demonstrated the presence of 3.01 μg/g sildenafil in one of the products and no sildenafil in the others.					
35616201	29	179	theme	%	4251:4251	arg1	range					4234:4238	the range	4230:4238	the range of 2.8%-5.3%	4230:4251	The recoveries of this SPE method ranged from 74.93% to 89.12%, with RSDs in the range of 2.8%-5.3%, proving the feasibility of this analytical method.					
35616201	26	180	theme	@	3890:3890	arg1	aerogel					3908:3914	the UiO-66-NH2@cellulose hybrid aerogel	3876:3914	the UiO-66-NH2@cellulose hybrid aerogel	3876:3914	The used composite aerogels were freeze-dried again and reused to extract sildenafil; the recovery was still maintained at 85.23% after five extraction cycles, indicating that the UiO-66-NH2@cellulose hybrid aerogel had good regeneration ability.					
35616201	22	181	theme	peak	3130:3133	arg1	proportional					3144:3155	proportional	3144:3155	proportional	3144:3155	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	22	181	theme	peak	3130:3133	arg1	area					3135:3138	The peak area	3126:3138	The peak area	3126:3138	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	12	182	theme	CMC-NHNH2	1650:1658	arg1	solution					1660:1667	the CMC-NHNH2 solution	1646:1667	the CMC-NHNH2 solution	1646:1667	Subsequently, UiO-66-NH2 was added to the CNC-CHO solution, mixed with the CMC-NHNH2 solution, and then crosslinked to load UiO-66-NH2 and form a hybrid aerogel as a bulk adsorbent.					
35616201	3	183	theme	sildenafil	380:389	arg1	detection					367:375	the detection	363:375	the detection of sildenafil in health products	363:408	Therefore, it is imperative to develop a method for the detection of sildenafil in health products.					
35616201	4	184	theme	samples	510:516	arg1	purification					478:489	purification	478:489	purification	478:489	Solid-phase extraction (SPE) is typically used for the separation, purification, and enrichment of samples, with the aim of reducing the matrix interference of the samples and improving the detection sensitivity.					
35616201	4	184	theme	samples	510:516	arg1	enrichment					496:505	enrichment	496:505	enrichment	496:505	Solid-phase extraction (SPE) is typically used for the separation, purification, and enrichment of samples, with the aim of reducing the matrix interference of the samples and improving the detection sensitivity.					
35616201	4	184	theme	samples	510:516	arg1	separation					466:475	separation	466:475	separation	466:475	Solid-phase extraction (SPE) is typically used for the separation, purification, and enrichment of samples, with the aim of reducing the matrix interference of the samples and improving the detection sensitivity.					
35616201	21	185	theme	adsorbent	3065:3073	arg1	aerogel					3042:3048	the UiO-66-NH2@cellulose hybrid aerogel	3010:3048	the UiO-66-NH2@cellulose hybrid aerogel	3010:3048	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	21	185	theme	adsorbent	3065:3073	arg1	sildenafil					3086:3095	an adsorbent to extract sildenafil	3062:3095	an adsorbent to extract sildenafil in different concentrations	3062:3123	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	21	186	theme	@	3024:3024	arg1	sildenafil					3086:3095	an adsorbent to extract sildenafil	3062:3095	an adsorbent to extract sildenafil in different concentrations	3062:3123	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	21	186	theme	@	3024:3024	arg1	aerogel					3042:3048	the UiO-66-NH2@cellulose hybrid aerogel	3010:3048	the UiO-66-NH2@cellulose hybrid aerogel	3010:3048	Under the optimal conditions, the UiO-66-NH2@cellulose hybrid aerogel was used as an adsorbent to extract sildenafil in different concentrations.					
35616201	7	187	theme	area	866:869	arg1	advantages					827:836	the advantages	823:836	the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability	823:932	MOFs offer the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability.					
35616201	9	188	theme	UiO-66-NH2	1252:1261	arg1	aerogel					1280:1286	a UiO-66-NH2@cellulose hybrid aerogel	1250:1286	a UiO-66-NH2@cellulose hybrid aerogel	1250:1286	Hence, this study proposes a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products.					
35616201	12	189	theme	load	1694:1697	arg1	UiO-66-NH2					1699:1708	load UiO-66-NH2	1694:1708	load UiO-66-NH2	1694:1708	Subsequently, UiO-66-NH2 was added to the CNC-CHO solution, mixed with the CMC-NHNH2 solution, and then crosslinked to load UiO-66-NH2 and form a hybrid aerogel as a bulk adsorbent.					
35616201	6	190	theme	organic	795:801	arg1	ligands					803:809	organic ligands	795:809	organic ligands	795:809	Metal-organic frameworks (MOFs) are hybrid crystalline materials composed of metal ions and organic ligands.					
35616201	15	191	theme	hybrid	2127:2132	arg1	aerogel					2134:2140	the hybrid aerogel	2123:2140	the hybrid aerogel	2123:2140	The results showed that UiO-66-NH2 was successfully loaded on the pore surface of the hybrid aerogel, which made the pore of aerogel become more regular and a larger surface area.					
35616201	26	192	theme	hybrid	3901:3906	arg1	aerogel					3908:3914	the UiO-66-NH2@cellulose hybrid aerogel	3876:3914	the UiO-66-NH2@cellulose hybrid aerogel	3876:3914	The used composite aerogels were freeze-dried again and reused to extract sildenafil; the recovery was still maintained at 85.23% after five extraction cycles, indicating that the UiO-66-NH2@cellulose hybrid aerogel had good regeneration ability.					
35616201	14	193	theme	synthesized	1848:1858	arg1	aerogel					1867:1873	The synthesized hybrid aerogel	1844:1873	The synthesized hybrid aerogel	1844:1873	The synthesized hybrid aerogel was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform-infrared (FT-IR) spectroscopy, and nitrogen gas adsorption/desorption.					
35616201	13	194	theme	extra	1816:1820	arg1	force					1822:1826	extra force	1816:1826	extra force for use in SPE	1816:1841	This bulk hybrid aerogel could be collected easily without extra force for use in SPE.					
35616201	7	195	theme	specific	849:856	arg1	area					866:869	a large specific surface area	841:869	a large specific surface area	841:869	MOFs offer the advantages of a large specific surface area, permanent nanopores, good stability, and pore controllability.					
35616201	9	196	theme	cellulose	1263:1271	arg1	aerogel					1280:1286	a UiO-66-NH2@cellulose hybrid aerogel	1250:1286	a UiO-66-NH2@cellulose hybrid aerogel	1250:1286	Hence, this study proposes a novel SPE method with high sensitivity that employs a UiO-66-NH2@cellulose hybrid aerogel, for the detection of sildenafil in health products.					
35616201	16	197	theme	better	2349:2354	arg1	efficiency					2367:2376	better extraction efficiency	2349:2376	better extraction efficiency	2349:2376	Investigation of the loading amount of UiO-66-NH2 in the hybrid aerogel revealed that a higher amount of UiO-66-NH2 could yield better extraction efficiency.					
35616201	4	198	theme	samples	575:581	arg1	interference					555:566	the matrix interference	544:566	the matrix interference of the samples	544:581	Solid-phase extraction (SPE) is typically used for the separation, purification, and enrichment of samples, with the aim of reducing the matrix interference of the samples and improving the detection sensitivity.					
35616201	24	199	theme	preconcentration	3369:3384	arg1	methods					3386:3392	previous preconcentration methods	3360:3392	previous preconcentration methods for sildenafil	3360:3407	Compared to previous preconcentration methods for sildenafil, this method offered a wider linear range.					
35616201	25	200	theme	extract	3551:3557	arg1	sildenafil					3559:3568	extract sildenafil	3551:3568	extract sildenafil	3551:3568	Five batches of hybrid aerogels were simultaneously prepared under the same conditions and used to extract sildenafil; the relative standard deviation (RSD, n=3) was 1.71%, indicating that the prepared hybrid aerogels offered good reproducibility.					
35616201	22	201	theme	59.17	3296:3300	arg1	detection					3234:3242	detection	3234:3242	detection (LOD, S/N=3) of 2.85 ng/mL	3234:3269	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
35616201	22	201	theme	59.17	3296:3300	arg1	factor					3286:3291	enrichment factor	3275:3291	enrichment factor	3275:3291	The peak area was proportional to the sildenafil concentration in the range of 10-2000 ng/mL, with limit of detection (LOD, S/N=3) of 2.85 ng/mL and enrichment factor of 59.17.					
36357204	10	0	dep	=	1976:1976	arg1	0.81					1978:1981	0.81	1978:1981	0.81	1978:1981	The model based on the detailed MFA profile determined by GC analysis did not outperform (AUCROC = 0.81) the model using the DHI-features in combination with BHB [MIR] and MFA [MIR].					
36357204	10	1	theme	detailed	1902:1909	arg1	profile					1915:1921	the detailed MFA profile	1898:1921	the detailed MFA profile determined by GC analysis	1898:1947	The model based on the detailed MFA profile determined by GC analysis did not outperform (AUCROC = 0.81) the model using the DHI-features in combination with BHB [MIR] and MFA [MIR].					
36357204	6	2	theme	data	934:937	arg1	leakage					939:945	any data leakage	930:945	any data leakage	930:945	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	9	3	theme	DHI	1775:1777	arg1	model					1779:1783	the DHI model	1771:1783	the DHI model	1771:1783	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	9	4	theme	model	1779:1783	arg1	features					1759:1766	the features	1755:1766	the features of the DHI model	1755:1783	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	7	5	theme	milk	969:972	arg1	MFA					987:989	MFA	987:989	MFA	987:989	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	5	theme	milk	969:972	arg1	acids					980:984	either milk fatty acids	962:984	either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR]	962:1396	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	6	6	from	data	916:919	arg1	present					884:890	present	884:890	present	884:890	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	12	7	theme	High	2238:2241	arg1	performance					2254:2264	High predictive performance	2238:2264	High predictive performance	2238:2264	High predictive performance was found using samples from d 9 and 21.					
36357204	11	8	from	d	2101:2101	arg1	samples					2090:2096	samples	2090:2096	samples at d 3	2090:2103	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	13	9	theme	[MIR	2355:2358	arg1	model					2361:2365	the DHI + BHB [MIR] + MFA [MIR] model	2329:2365	model	2361:2365	To conclude, overall, the DHI + BHB [MIR] + MFA [MIR] model allowed to predict metabolic status during early lactation.					
36357204	9	10	theme	BHB	1790:1792	arg1	[MIR					1794:1797	BHB [MIR	1790:1797	BHB [MIR	1790:1797	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	3	11	theme	blood	464:468	arg1	parameters					470:479	the 5 blood parameters	458:479	the 5 blood parameters of all 4 sampling days	458:502	Clustering relied on the average and range of the 5 blood parameters of all 4 sampling days.					
36357204	11	12	theme	MFA	2208:2210	arg1	model					2217:2221	AUCROC MFA [GC] model	2201:2221	lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73)	2127:2235	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	11	13	from	d	2226:2226	arg1	model					2217:2221	AUCROC MFA [GC] model	2201:2221	lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73)	2127:2235	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	7	14	theme	urea	1196:1199	arg1	concentration					1137:1149	concentration	1137:1149	concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations)	1137:1280	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	8	15	theme	somatic	1569:1575	arg1	count					1582:1586	somatic cell count	1569:1586	somatic cell count	1569:1586	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
36357204	7	16	theme	gas	1006:1008	arg1	chromatography					1010:1023	gas chromatography	1006:1023	gas chromatography (MFA [GC])	1006:1034	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	16	theme	gas	1006:1008	arg1	[GC					1030:1032	MFA [GC	1026:1032	MFA [GC	1026:1032	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	17	theme	count	1219:1223	arg1	concentration					1137:1149	concentration	1137:1149	concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations)	1137:1280	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	18	theme	ratio	1189:1193	arg1	concentration					1137:1149	concentration	1137:1149	concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations)	1137:1280	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	9	19	theme	MFA	1804:1806	arg1	[MIR					1808:1811	MFA [MIR	1804:1811	MFA [MIR	1804:1811	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	6	20	attach	present	884:890	arg1	train					902:906	train	902:906	train	902:906	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	6	20	attach	present	884:890	arg2	data					849:852	meaning data	841:852	meaning data from a single cow	841:870	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	6	20	attach	present	884:890	arg1	data					916:919	test data	911:919	test data	911:919	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	8	21	theme	fat/protein	1541:1551	arg1	ratio					1553:1557	fat/protein ratio	1541:1557	fat/protein ratio	1541:1557	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
36357204	7	22	theme	DHI	1472:1474	arg1	registrations					1476:1488	DHI registrations	1472:1488	DHI registrations	1472:1488	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	9	23	with	combination	1740:1750	arg1	[MIR					1794:1797	BHB [MIR	1790:1797	BHB [MIR	1790:1797	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	5	24	theme	metabolic	693:701	arg1	cluster					703:709	the metabolic cluster	689:709	the metabolic cluster of cows	689:717	Various random forest models were built to predict the metabolic cluster of cows during early lactation from the milk composition.					
36357204	6	25	theme	leave-group-out	807:821	arg1	cross-validation					823:838	a leave-group-out cross-validation	805:838	a leave-group-out cross-validation	805:838	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	4	26	theme	=	548:548	arg1	n					546:546	n = 42	546:551	n = 42	546:551	The clusters were labeled as imbalanced (n = 42) and balanced (n = 72) metabolic status based on the values of the blood parameters.					
36357204	4	26	theme	=	548:548	arg1	imbalanced					534:543	imbalanced	534:543	imbalanced	534:543	The clusters were labeled as imbalanced (n = 42) and balanced (n = 72) metabolic status based on the values of the blood parameters.					
36357204	8	27	theme	DHI	1595:1597	arg1	model					1599:1603	DHI model	1595:1603	DHI model	1595:1603	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
36357204	8	27	theme	DHI	1595:1597	arg1	fat					1518:1520	fat	1518:1520	fat	1518:1520	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
36357204	12	28	from	d	2295:2295	arg1	samples					2282:2288	samples	2282:2288	samples from d 9 and 21	2282:2304	High predictive performance was found using samples from d 9 and 21.					
36357204	7	29	with	combination	1300:1310	arg1	MFA					1317:1319	MFA	1317:1319	MFA	1317:1319	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	29	with	combination	1300:1310	arg1	BHB					1325:1327	BHB	1325:1327	BHB	1325:1327	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	30	dep	fat	1154:1156	arg1	reported					1241:1248	reported	1241:1248	reported routinely in DHI registrations	1241:1279	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	30	dep	fat	1154:1156	arg1	determined					1226:1235	determined	1226:1235	determined	1226:1235	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	8	31	theme	operating	1687:1695	arg1	curve					1712:1716	the receiver operating characteristic curve	1674:1716	the receiver operating characteristic curve	1674:1716	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
36357204	4	32	theme	metabolic	576:584	arg1	status					586:591	metabolic status	576:591	metabolic status	576:591	The clusters were labeled as imbalanced (n = 42) and balanced (n = 72) metabolic status based on the values of the blood parameters.					
36357204	9	33	theme	predictive	1839:1848	arg1	0.81					1872:1875	AUCROC = 0.81	1863:1875	AUCROC = 0.81	1863:1875	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	9	33	theme	predictive	1839:1848	arg1	performance					1850:1860	the predictive performance	1835:1860	the predictive performance (AUCROC = 0.81)	1835:1876	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	3	34	theme	days	499:502	arg1	parameters					470:479	the 5 blood parameters	458:479	the 5 blood parameters of all 4 sampling days	458:502	Clustering relied on the average and range of the 5 blood parameters of all 4 sampling days.					
36357204	9	35	with	[MIR	1808:1811	arg1	[MIR					1794:1797	BHB [MIR	1790:1797	BHB [MIR	1790:1797	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	10	36	from	DHI-features	2004:2015	arg1	combination					2020:2030	combination	2020:2030	combination with BHB [MIR] and MFA [MIR	2020:2058	The model based on the detailed MFA profile determined by GC analysis did not outperform (AUCROC = 0.81) the model using the DHI-features in combination with BHB [MIR] and MFA [MIR].					
36357204	9	37	theme	=	1870:1870	arg1	0.81					1872:1875	AUCROC = 0.81	1863:1875	AUCROC = 0.81	1863:1875	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	9	37	theme	=	1870:1870	arg1	performance					1850:1860	the predictive performance	1835:1860	the predictive performance (AUCROC = 0.81)	1835:1876	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	14	38	theme	status	2508:2513	arg1	prediction					2484:2493	routine prediction	2476:2493	routine prediction of metabolic status	2476:2513	Accordingly, these parameters show potential for routine prediction of metabolic status.					
36357204	1	39	from	d	157:157	arg1	lactation					178:186	lactation	178:186	lactation	178:186	Data on metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows) were used for unsupervised k-means clustering.					
36357204	1	40	theme	blood	140:144	arg1	profiles					128:135	metabolic profiles	118:135	metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows)	118:216	Data on metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows) were used for unsupervised k-means clustering.					
36357204	8	41	theme	predictive	1639:1648	arg1	[area					1662:1666	the lowest predictive performance [area	1628:1666	the lowest predictive performance [area under the receiver operating characteristic curve	1628:1716	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
36357204	6	42	from	present	884:890	arg1	train					902:906	train	902:906	train	902:906	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	6	42	from	present	884:890	arg1	data					916:919	test data	911:919	test data	911:919	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	4	43	theme	blood	620:624	arg1	parameters					626:635	the blood parameters	616:635	the blood parameters	616:635	The clusters were labeled as imbalanced (n = 42) and balanced (n = 72) metabolic status based on the values of the blood parameters.					
36357204	14	44	theme	routine	2476:2482	arg1	prediction					2484:2493	routine prediction	2476:2493	routine prediction of metabolic status	2476:2513	Accordingly, these parameters show potential for routine prediction of metabolic status.					
36357204	11	45	theme	model	2217:2221	arg1	performance					2133:2143	lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model	2127:2185	performance	2133:2143	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	2	46	theme	fatty	340:344	arg1	acids					346:350	nonesterified fatty acids	326:350	nonesterified fatty acids	326:350	Blood metabolic parameters included β-hydroxybutyrate (BHB), nonesterified fatty acids, glucose, insulin-like growth factor-1 (IGF-1) and insulin.					
36357204	0	47	theme	Diagnostic	0:9	arg1	biomarkers					16:25	Diagnostic milk biomarkers	0:25	Diagnostic milk biomarkers for predicting the metabolic health status of dairy cattle during early lactation.	0:108	Diagnostic milk biomarkers for predicting the metabolic health status of dairy cattle during early lactation.					
36357204	11	48	theme	lower	2127:2131	arg1	performance					2133:2143	lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model	2127:2185	performance	2133:2143	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	1	49	from	Data	110:113	arg1	profiles					128:135	metabolic profiles	118:135	metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows)	118:216	Data on metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows) were used for unsupervised k-means clustering.					
36357204	3	50	dep	average	437:443	arg1	the					433:435	the	433:435	the	433:435	Clustering relied on the average and range of the 5 blood parameters of all 4 sampling days.					
36357204	7	51	theme	BHB	1388:1390	arg1	[MIR					1392:1395	a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR	1080:1395	[MIR	1392:1395	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	6	52	theme	test	911:914	arg1	data					916:919	test data	911:919	test data	911:919	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	2	53	theme	growth	375:380	arg1	factor-1					382:389	insulin-like growth factor-1	362:389	insulin-like growth factor-1 (IGF-1)	362:397	Blood metabolic parameters included β-hydroxybutyrate (BHB), nonesterified fatty acids, glucose, insulin-like growth factor-1 (IGF-1) and insulin.					
36357204	2	53	theme	growth	375:380	arg1	IGF-1					392:396	IGF-1	392:396	IGF-1	392:396	Blood metabolic parameters included β-hydroxybutyrate (BHB), nonesterified fatty acids, glucose, insulin-like growth factor-1 (IGF-1) and insulin.					
36357204	7	54	dep	analyzed	1433:1440	arg1	reported					1460:1467	reported	1460:1467	reported in DHI registrations yet	1460:1492	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	5	55	theme	forest	653:658	arg1	models					660:665	Various random forest models	638:665	Various random forest models	638:665	Various random forest models were built to predict the metabolic cluster of cows during early lactation from the milk composition.					
36357204	10	56	theme	BHB	2037:2039	arg1	[MIR					2041:2044	BHB [MIR]	2037:2045	BHB [MIR]	2037:2045	The model based on the detailed MFA profile determined by GC analysis did not outperform (AUCROC = 0.81) the model using the DHI-features in combination with BHB [MIR] and MFA [MIR].					
36357204	7	57	theme	DHI	1114:1116	arg1	analysis					1119:1126	a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR	1080:1395	analysis	1119:1126	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	8	58	dep	model	1599:1603	arg1	i.e.					1589:1592	i.e.	1589:1592	i.e.	1589:1592	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
36357204	5	59	theme	random	646:651	arg1	models					660:665	Various random forest models	638:665	Various random forest models	638:665	Various random forest models were built to predict the metabolic cluster of cows during early lactation from the milk composition.					
36357204	7	60	dep	analysis	1119:1126	arg1	such					1129:1132	such	1129:1132	such	1129:1132	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	61	theme	improvement	1101:1111	arg1	analysis					1119:1126	a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR	1080:1395	analysis	1119:1126	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	11	62	theme	0.75	2195:2198	arg1	performance					2133:2143	lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model	2127:2185	performance	2133:2143	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	13	63	theme	BHB	2339:2341	arg1	[MIR					2343:2346	the DHI + BHB [MIR] + MFA [MIR] model	2329:2365	[MIR	2343:2346	To conclude, overall, the DHI + BHB [MIR] + MFA [MIR] model allowed to predict metabolic status during early lactation.					
36357204	0	64	theme	metabolic	46:54	arg1	status					63:68	the metabolic health status	42:68	the metabolic health status of dairy cattle	42:84	Diagnostic milk biomarkers for predicting the metabolic health status of dairy cattle during early lactation.					
36357204	1	65	theme	k-means	245:251	arg1	clustering					253:262	unsupervised k-means clustering	232:262	unsupervised k-means clustering	232:262	Data on metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows) were used for unsupervised k-means clustering.					
36357204	4	66	dep	imbalanced	534:543	arg1	status					586:591	metabolic status	576:591	metabolic status	576:591	The clusters were labeled as imbalanced (n = 42) and balanced (n = 72) metabolic status based on the values of the blood parameters.					
36357204	7	67	theme	dairy	1090:1094	arg1	analysis					1119:1126	a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR	1080:1395	analysis	1119:1126	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	2	68	theme	Blood	265:269	arg1	parameters					281:290	Blood metabolic parameters	265:290	Blood metabolic parameters	265:290	Blood metabolic parameters included β-hydroxybutyrate (BHB), nonesterified fatty acids, glucose, insulin-like growth factor-1 (IGF-1) and insulin.					
36357204	7	69	theme	DHI	1263:1265	arg1	registrations					1267:1279	DHI registrations	1263:1279	DHI registrations	1263:1279	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	11	70	theme	BHB	2159:2161	arg1	[MIR					2163:2166	BHB [MIR	2159:2166	BHB [MIR	2159:2166	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	0	71	theme	health	56:61	arg1	status					63:68	the metabolic health status	42:68	the metabolic health status of dairy cattle	42:84	Diagnostic milk biomarkers for predicting the metabolic health status of dairy cattle during early lactation.					
36357204	8	72	theme	performance	1650:1660	arg1	[area					1662:1666	the lowest predictive performance [area	1628:1666	the lowest predictive performance [area under the receiver operating characteristic curve	1628:1716	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
36357204	10	73	dep	AUCROC	1969:1974	arg1	model					1988:1992	the model	1984:1992	the model using the DHI-features in combination with BHB [MIR] and MFA [MIR	1984:2058	The model based on the detailed MFA profile determined by GC analysis did not outperform (AUCROC = 0.81) the model using the DHI-features in combination with BHB [MIR] and MFA [MIR].					
36357204	13	74	theme	MFA	2351:2353	arg1	model					2361:2365	the DHI + BHB [MIR] + MFA [MIR] model	2329:2365	model	2361:2365	To conclude, overall, the DHI + BHB [MIR] + MFA [MIR] model allowed to predict metabolic status during early lactation.					
36357204	11	75	theme	MFA	2171:2173	arg1	model					2181:2185	lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model	2127:2185	model	2181:2185	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	1	76	used	used	223:226	arg2	Data					110:113	Data	110:113	Data on metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows)	110:216	Data on metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows) were used for unsupervised k-means clustering.					
36357204	1	76	used	used	223:226	arg2	profiles					128:135	metabolic profiles	118:135	metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows)	118:216	Data on metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows) were used for unsupervised k-means clustering.					
36357204	12	77	theme	predictive	2243:2252	arg1	performance					2254:2264	High predictive performance	2238:2264	High predictive performance	2238:2264	High predictive performance was found using samples from d 9 and 21.					
36357204	7	78	theme	somatic	1206:1212	arg1	count					1219:1223	somatic cell count	1206:1223	somatic cell count	1206:1223	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	8	79	theme	cell	1577:1580	arg1	count					1582:1586	somatic cell count	1569:1586	somatic cell count	1569:1586	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
36357204	5	80	theme	cows	714:717	arg1	cluster					703:709	the metabolic cluster	689:709	the metabolic cluster of cows	689:717	Various random forest models were built to predict the metabolic cluster of cows during early lactation from the milk composition.					
36357204	7	81	theme	cell	1214:1217	arg1	count					1219:1223	somatic cell count	1206:1223	somatic cell count	1206:1223	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	13	82	theme	early	2410:2414	arg1	lactation					2416:2424	early lactation	2410:2424	early lactation	2410:2424	To conclude, overall, the DHI + BHB [MIR] + MFA [MIR] model allowed to predict metabolic status during early lactation.					
36357204	0	83	theme	dairy	73:77	arg1	cattle					79:84	dairy cattle	73:84	dairy cattle	73:84	Diagnostic milk biomarkers for predicting the metabolic health status of dairy cattle during early lactation.					
36357204	6	84	theme	meaning	841:847	arg1	data					849:852	meaning data	841:852	meaning data from a single cow	841:870	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	5	85	theme	early	726:730	arg1	lactation					732:740	early lactation	726:740	early lactation from the milk composition	726:766	Various random forest models were built to predict the metabolic cluster of cows during early lactation from the milk composition.					
36357204	7	86	theme	MFA	1374:1376	arg1	[MIR					1378:1381	MFA [MIR	1374:1381	MFA [MIR	1374:1381	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	10	87	theme	MFA	1911:1913	arg1	profile					1915:1921	the detailed MFA profile	1898:1921	the detailed MFA profile determined by GC analysis	1898:1947	The model based on the detailed MFA profile determined by GC analysis did not outperform (AUCROC = 0.81) the model using the DHI-features in combination with BHB [MIR] and MFA [MIR].					
36357204	6	88	from	train	902:906	arg1	present					884:890	present	884:890	present	884:890	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	3	89	theme	parameters	470:479	arg1	average					437:443	average	437:443	average	437:443	Clustering relied on the average and range of the 5 blood parameters of all 4 sampling days.					
36357204	3	89	theme	parameters	470:479	arg1	range					449:453	range	449:453	range	449:453	Clustering relied on the average and range of the 5 blood parameters of all 4 sampling days.					
36357204	8	90	dep	=	1727:1727	arg1	0.69					1729:1732	0.69	1729:1732	0.69	1729:1732	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
36357204	7	91	theme	fat/protein	1177:1187	arg1	ratio					1189:1193	fat/protein ratio	1177:1193	fat/protein ratio	1177:1193	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	10	92	theme	GC	1937:1938	arg1	analysis					1940:1947	GC analysis	1937:1947	GC analysis	1937:1947	The model based on the detailed MFA profile determined by GC analysis did not outperform (AUCROC = 0.81) the model using the DHI-features in combination with BHB [MIR] and MFA [MIR].					
36357204	7	93	theme	lactose	1168:1174	arg1	concentration					1137:1149	concentration	1137:1149	concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations)	1137:1280	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	94	theme	MFA	1026:1028	arg1	chromatography					1010:1023	gas chromatography	1006:1023	gas chromatography (MFA [GC])	1006:1034	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	94	theme	MFA	1026:1028	arg1	[GC					1030:1032	MFA [GC	1026:1032	MFA [GC	1026:1032	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	3	95	theme	sampling	490:497	arg1	days					499:502	all 4 sampling days	484:502	all 4 sampling days	484:502	Clustering relied on the average and range of the 5 blood parameters of all 4 sampling days.					
36357204	10	96	with	combination	2020:2030	arg1	[MIR					2041:2044	BHB [MIR]	2037:2045	BHB [MIR]	2037:2045	The model based on the detailed MFA profile determined by GC analysis did not outperform (AUCROC = 0.81) the model using the DHI-features in combination with BHB [MIR] and MFA [MIR].					
36357204	10	96	with	combination	2020:2030	arg1	[MIR					2055:2058	MFA [MIR	2051:2058	MFA [MIR	2051:2058	The model based on the detailed MFA profile determined by GC analysis did not outperform (AUCROC = 0.81) the model using the DHI-features in combination with BHB [MIR] and MFA [MIR].					
36357204	6	97	dep	cross-validation	823:838	arg1	present					884:890	present	884:890	present	884:890	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	7	98	theme	protein	1159:1165	arg1	concentration					1137:1149	concentration	1137:1149	concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations)	1137:1280	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	11	99	theme	[GC	2212:2214	arg1	model					2217:2221	AUCROC MFA [GC] model	2201:2221	lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73)	2127:2235	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	1	100	theme	metabolic	118:126	arg1	profiles					128:135	metabolic profiles	118:135	metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows)	118:216	Data on metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows) were used for unsupervised k-means clustering.					
36357204	7	101	theme	fat	1154:1156	arg1	concentration					1137:1149	concentration	1137:1149	concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations)	1137:1280	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	11	102	theme	AUCROC	2201:2206	arg1	model					2217:2221	AUCROC MFA [GC] model	2201:2221	lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73)	2127:2235	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	9	103	theme	AUCROC	1863:1868	arg1	0.81					1872:1875	AUCROC = 0.81	1863:1875	AUCROC = 0.81	1863:1875	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	9	103	theme	AUCROC	1863:1868	arg1	performance					1850:1860	the predictive performance	1835:1860	the predictive performance (AUCROC = 0.81)	1835:1876	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	8	104	theme	lowest	1632:1637	arg1	[area					1662:1666	the lowest predictive performance [area	1628:1666	the lowest predictive performance [area under the receiver operating characteristic curve	1628:1716	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
36357204	0	105	theme	cattle	79:84	arg1	status					63:68	the metabolic health status	42:68	the metabolic health status of dairy cattle	42:84	Diagnostic milk biomarkers for predicting the metabolic health status of dairy cattle during early lactation.					
36357204	11	106	from	d	2190:2190	arg1	performance					2133:2143	lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model	2127:2185	performance	2133:2143	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	5	107	theme	milk	751:754	arg1	composition					756:766	the milk composition	747:766	the milk composition	747:766	Various random forest models were built to predict the metabolic cluster of cows during early lactation from the milk composition.					
36357204	6	108	theme	single	861:866	arg1	cow					868:870	a single cow	859:870	a single cow	859:870	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	6	109	from	cow	868:870	arg1	data					849:852	meaning data	841:852	meaning data from a single cow	841:870	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	4	110	theme	=	570:570	arg1	n					568:568	n = 72	568:573	n = 72	568:573	The clusters were labeled as imbalanced (n = 42) and balanced (n = 72) metabolic status based on the values of the blood parameters.					
36357204	4	110	theme	=	570:570	arg1	balanced					558:565	balanced	558:565	balanced	558:565	The clusters were labeled as imbalanced (n = 42) and balanced (n = 72) metabolic status based on the values of the blood parameters.					
36357204	0	111	theme	early	93:97	arg1	lactation					99:107	early lactation	93:107	early lactation	93:107	Diagnostic milk biomarkers for predicting the metabolic health status of dairy cattle during early lactation.					
36357204	5	112	from	composition	756:766	arg1	lactation					732:740	early lactation	726:740	early lactation from the milk composition	726:766	Various random forest models were built to predict the metabolic cluster of cows during early lactation from the milk composition.					
36357204	11	113	dep	performance	2133:2143	arg1	DHI					2153:2155	AUCROC DHI	2146:2155	AUCROC DHI	2146:2155	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	11	113	dep	performance	2133:2143	arg1	[MIR					2163:2166	BHB [MIR	2159:2166	BHB [MIR	2159:2166	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	7	114	dep	concentration	1137:1149	arg1	either					1283:1288	either	1283:1288	either	1283:1288	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	115	dep	acids	980:984	arg1	not					1446:1448	not	1446:1448	not	1446:1448	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	115	dep	acids	980:984	arg1	analyzed					1433:1440	analyzed	1433:1440	analyzed	1433:1440	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	4	116	theme	parameters	626:635	arg1	values					606:611	the values	602:611	the values of the blood parameters	602:635	The clusters were labeled as imbalanced (n = 42) and balanced (n = 72) metabolic status based on the values of the blood parameters.					
36357204	2	117	theme	nonesterified	326:338	arg1	acids					346:350	nonesterified fatty acids	326:350	nonesterified fatty acids	326:350	Blood metabolic parameters included β-hydroxybutyrate (BHB), nonesterified fatty acids, glucose, insulin-like growth factor-1 (IGF-1) and insulin.					
36357204	0	118	theme	milk	11:14	arg1	biomarkers					16:25	Diagnostic milk biomarkers	0:25	Diagnostic milk biomarkers for predicting the metabolic health status of dairy cattle during early lactation.	0:108	Diagnostic milk biomarkers for predicting the metabolic health status of dairy cattle during early lactation.					
36357204	12	119	from	21	2303:2304	arg1	samples					2282:2288	samples	2282:2288	samples from d 9 and 21	2282:2304	High predictive performance was found using samples from d 9 and 21.					
36357204	14	120	theme	metabolic	2498:2506	arg1	status					2508:2513	metabolic status	2498:2513	metabolic status	2498:2513	Accordingly, these parameters show potential for routine prediction of metabolic status.					
36357204	8	121	dep	characterized	1611:1623	arg1	=					1727:1727	=	1727:1727	=	1727:1727	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
36357204	6	122	located	present	884:890	arg1	train					902:906	train	902:906	train	902:906	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	6	122	located	present	884:890	arg2	data					849:852	meaning data	841:852	meaning data from a single cow	841:870	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	6	122	located	present	884:890	arg1	data					916:919	test data	911:919	test data	911:919	All the models were evaluated using a leave-group-out cross-validation, meaning data from a single cow were always present in either train or test data to avoid any data leakage.					
36357204	2	123	theme	insulin-like	362:373	arg1	factor-1					382:389	insulin-like growth factor-1	362:389	insulin-like growth factor-1 (IGF-1)	362:397	Blood metabolic parameters included β-hydroxybutyrate (BHB), nonesterified fatty acids, glucose, insulin-like growth factor-1 (IGF-1) and insulin.					
36357204	2	123	theme	insulin-like	362:373	arg1	IGF-1					392:396	IGF-1	392:396	IGF-1	392:396	Blood metabolic parameters included β-hydroxybutyrate (BHB), nonesterified fatty acids, glucose, insulin-like growth factor-1 (IGF-1) and insulin.					
36357204	11	124	theme	[MIR	2175:2178	arg1	model					2181:2185	lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model	2127:2185	model	2181:2185	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	5	125	theme	Various	638:644	arg1	models					660:665	Various random forest models	638:665	Various random forest models	638:665	Various random forest models were built to predict the metabolic cluster of cows during early lactation from the milk composition.					
36357204	13	126	theme	metabolic	2386:2394	arg1	status					2396:2401	metabolic status	2386:2401	metabolic status	2386:2401	To conclude, overall, the DHI + BHB [MIR] + MFA [MIR] model allowed to predict metabolic status during early lactation.					
36357204	7	127	theme	herd	1096:1099	arg1	analysis					1119:1126	a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR	1080:1395	analysis	1119:1126	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	10	128	theme	MFA	2051:2053	arg1	[MIR					2055:2058	MFA [MIR	2051:2058	MFA [MIR	2051:2058	The model based on the detailed MFA profile determined by GC analysis did not outperform (AUCROC = 0.81) the model using the DHI-features in combination with BHB [MIR] and MFA [MIR].					
36357204	7	129	theme	fatty	974:978	arg1	MFA					987:989	MFA	987:989	MFA	987:989	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	7	129	theme	fatty	974:978	arg1	acids					980:984	either milk fatty acids	962:984	either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR]	962:1396	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	1	130	theme	unsupervised	232:243	arg1	clustering					253:262	unsupervised k-means clustering	232:262	unsupervised k-means clustering	232:262	Data on metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows) were used for unsupervised k-means clustering.					
36357204	10	131	dep	outperform	1957:1966	arg1	AUCROC					1969:1974	AUCROC	1969:1974	AUCROC	1969:1974	The model based on the detailed MFA profile determined by GC analysis did not outperform (AUCROC = 0.81) the model using the DHI-features in combination with BHB [MIR] and MFA [MIR].					
36357204	7	132	theme	routine	1082:1088	arg1	analysis					1119:1126	a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR	1080:1395	analysis	1119:1126	Features were either milk fatty acids (MFA) determined by gas chromatography (MFA [GC]) or features that could be determined during a routine dairy herd improvement (DHI) analysis, such as concentration of fat, protein, lactose, fat/protein ratio, urea, and somatic cell count (determined and reported routinely in DHI registrations), either or not in combination with MFA and BHB determined by mid-infrared (MIR), denoted as MFA [MIR] and BHB [MIR], respectively, which are routinely analyzed but not routinely reported in DHI registrations yet.					
36357204	2	133	theme	metabolic	271:279	arg1	parameters					281:290	Blood metabolic parameters	265:290	Blood metabolic parameters	265:290	Blood metabolic parameters included β-hydroxybutyrate (BHB), nonesterified fatty acids, glucose, insulin-like growth factor-1 (IGF-1) and insulin.					
36357204	9	134	theme	features	1759:1766	arg1	combination					1740:1750	The combination	1736:1750	The combination of the features of the DHI model with BHB [MIR	1736:1797	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	9	134	theme	features	1759:1766	arg1	[MIR					1808:1811	MFA [MIR	1804:1811	MFA [MIR	1804:1811	The combination of the features of the DHI model with BHB [MIR] and MFA [MIR] powerfully increased the predictive performance (AUCROC = 0.81).					
36357204	11	135	theme	AUCROC	2146:2151	arg1	DHI					2153:2155	AUCROC DHI	2146:2155	AUCROC DHI	2146:2155	Predictions solely based on samples at d 3 were characterized by lower performance (AUCROC DHI + BHB [MIR] + MFA [MIR] model at d 3: 0.75; AUCROC MFA [GC] model at d 3: 0.73).					
36357204	8	136	theme	characteristic	1697:1710	arg1	curve					1712:1716	the receiver operating characteristic curve	1674:1716	the receiver operating characteristic curve	1674:1716	Models solely based on fat, protein, lactose, fat/protein ratio, urea and somatic cell count (i.e., DHI model) were characterized by the lowest predictive performance [area under the receiver operating characteristic curve (AUCROC) = 0.69].					
35088457	13	0	from	impact	2069:2074	arg1	health					2114:2119	intestinal health	2103:2119	intestinal health	2103:2119	These results indicated that application of IMO or CHE could favourably modulate the intestinal microbial composition of piglets, while their beneficial impact and molecular mechanism on intestinal health warrants further investigation.					
35088457	1	1	theme	intestinal	439:448	arg1	morphology					450:459	intestinal morphology	439:459	intestinal morphology	439:459	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	13	2	theme	further	2130:2136	arg1	investigation					2138:2150	further investigation	2130:2150	further investigation	2130:2150	These results indicated that application of IMO or CHE could favourably modulate the intestinal microbial composition of piglets, while their beneficial impact and molecular mechanism on intestinal health warrants further investigation.					
35088457	8	3	from	decrease	1328:1335	arg1	lipoprotein					1353:1363	high-density lipoprotein	1340:1363	high-density lipoprotein	1340:1363	Serum biochemical analysis showed that dietary CHE decreased aspartate aminotransferase levels, while inclusion of IMO led to a decrease in high-density lipoprotein.					
35088457	1	4	theme	herbal	278:283	arg1	medicine					285:292	Chinese herbal medicine	270:292	Chinese herbal medicine extract (CHE)	270:306	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	5	theme	current	190:196	arg1	study					198:202	the current study	186:202	the current study	186:202	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	6	theme	piglets	486:492	arg1	profiles					395:402	serum biochemical profiles	377:402	serum biochemical profiles	377:402	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	6	theme	piglets	486:492	arg1	morphology					450:459	intestinal morphology	439:459	intestinal morphology	439:459	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	6	theme	piglets	486:492	arg1	microflora					465:474	microflora	465:474	microflora	465:474	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	6	theme	piglets	486:492	arg1	incidence					366:374	diarrhoea incidence	356:374	diarrhoea incidence	356:374	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	6	theme	piglets	486:492	arg1	expression					427:436	inflammatory cytokine expression	405:436	inflammatory cytokine expression	405:436	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	6	theme	piglets	486:492	arg1	performance					343:353	growth performance	336:353	growth performance	336:353	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	13	7	theme	beneficial	2058:2067	arg1	impact					2069:2074	their beneficial impact	2052:2074	their beneficial impact	2052:2074	These results indicated that application of IMO or CHE could favourably modulate the intestinal microbial composition of piglets, while their beneficial impact and molecular mechanism on intestinal health warrants further investigation.					
35088457	5	8	theme	1 g/kg	830:835	arg1	CHE					837:839	1 g/kg CHE	830:839	1 g/kg CHE	830:839	Group III were fed diet with 0.5 g/kg CHE for 14 days and 1 g/kg CHE for another 14 days.					
35088457	1	9	theme	serum	377:381	arg1	profiles					395:402	serum biochemical profiles	377:402	serum biochemical profiles	377:402	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	6	10	theme	2 g/kg	891:896	arg1	CHE					913:915	2 g/kg IMO + 0.5 g/kg CHE	891:915	2 g/kg IMO + 0.5 g/kg CHE	891:915	Group IV were fed diet with (2 g/kg IMO + 0.5 g/kg CHE) for 14 days and (4 g/kg IMO +1 g/kg CHE) for another 14 days.					
35088457	6	10	theme	2 g/kg	891:896	arg1	diet					880:883	diet	880:883	diet with (2 g/kg IMO + 0.5 g/kg CHE) for 14 days and (4 g/kg IMO +1 g/kg CHE)	880:957	Group IV were fed diet with (2 g/kg IMO + 0.5 g/kg CHE) for 14 days and (4 g/kg IMO +1 g/kg CHE) for another 14 days.					
35088457	0	11	theme	early-weaned	147:158	arg1	piglets					160:166	early-weaned piglets	147:166	early-weaned piglets	147:166	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	13	12	theme	intestinal	2103:2112	arg1	health					2114:2119	intestinal health	2103:2119	intestinal health	2103:2119	These results indicated that application of IMO or CHE could favourably modulate the intestinal microbial composition of piglets, while their beneficial impact and molecular mechanism on intestinal health warrants further investigation.					
35088457	0	13	from	performance	67:77	arg1	piglets					160:166	early-weaned piglets	147:166	early-weaned piglets	147:166	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	8	14	theme	biochemical	1206:1216	arg1	analysis					1218:1225	Serum biochemical analysis	1200:1225	Serum biochemical analysis	1200:1225	Serum biochemical analysis showed that dietary CHE decreased aspartate aminotransferase levels, while inclusion of IMO led to a decrease in high-density lipoprotein.					
35088457	1	15	theme	inflammatory	405:416	arg1	expression					427:436	inflammatory cytokine expression	405:436	inflammatory cytokine expression	405:436	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	11	16	dep	piglets	1715:1721	arg1	the					1701:1703	the	1701:1703	the	1701:1703	Meanwhile, the combination of IMO and CHE significantly increased Bifidobacterium in the caecum of piglets.					
35088457	11	16	dep	piglets	1715:1721	arg1	caecum					1705:1710	caecum	1705:1710	caecum	1705:1710	Meanwhile, the combination of IMO and CHE significantly increased Bifidobacterium in the caecum of piglets.					
35088457	12	17	from	species	1889:1895	arg1	digesta					1907:1913	caecal digesta	1900:1913	caecal digesta	1900:1913	Additionally, dietary IMO, CHE or their combination significantly reduced the number of potential entero-pathogen Escherichia coli in ileal contents and Clostridium species in caecal digesta.					
35088457	6	18	dep	CHE	913:915	arg1	with					885:888	with	885:888	with	885:888	Group IV were fed diet with (2 g/kg IMO + 0.5 g/kg CHE) for 14 days and (4 g/kg IMO +1 g/kg CHE) for another 14 days.					
35088457	8	19	theme	dietary	1239:1245	arg1	CHE					1247:1249	dietary CHE	1239:1249	dietary CHE	1239:1249	Serum biochemical analysis showed that dietary CHE decreased aspartate aminotransferase levels, while inclusion of IMO led to a decrease in high-density lipoprotein.					
35088457	13	20	theme	piglets	2037:2043	arg1	composition					2022:2032	the intestinal microbial composition	1997:2032	the intestinal microbial composition of piglets	1997:2043	These results indicated that application of IMO or CHE could favourably modulate the intestinal microbial composition of piglets, while their beneficial impact and molecular mechanism on intestinal health warrants further investigation.					
35088457	6	21	theme	4 g/kg	935:940	arg1	CHE					954:956	4 g/kg IMO +1 g/kg CHE	935:956	(4 g/kg IMO +1 g/kg CHE)	934:957	Group IV were fed diet with (2 g/kg IMO + 0.5 g/kg CHE) for 14 days and (4 g/kg IMO +1 g/kg CHE) for another 14 days.					
35088457	0	22	theme	serum	80:84	arg1	profiles					98:105	serum biochemical profiles	80:105	serum biochemical profiles	80:105	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	1	23	theme	growth	336:341	arg1	performance					343:353	growth performance	336:353	growth performance	336:353	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	9	24	theme	potent	1463:1468	arg1	cytokine					1488:1495	a potent anti-inflammatory cytokine	1461:1495	a potent anti-inflammatory cytokine	1461:1495	Moreover, co-administration of IMO with CHE significantly upregulated the expression of TGF-β, a potent anti-inflammatory cytokine, in jejunal mucosa of piglets.					
35088457	9	24	theme	potent	1463:1468	arg1	TGF-β					1454:1458	TGF-β	1454:1458	TGF-β	1454:1458	Moreover, co-administration of IMO with CHE significantly upregulated the expression of TGF-β, a potent anti-inflammatory cytokine, in jejunal mucosa of piglets.					
35088457	0	25	from	Effects	0:6	arg1	populations					132:142	intestinal bacterial populations	111:142	intestinal bacterial populations	111:142	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	0	25	from	Effects	0:6	arg1	performance					67:77	growth performance	60:77	growth performance	60:77	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	0	25	from	Effects	0:6	arg1	profiles					98:105	serum biochemical profiles	80:105	serum biochemical profiles	80:105	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	8	26	theme	aminotransferase	1271:1286	arg1	levels					1288:1293	aspartate aminotransferase levels	1261:1293	aspartate aminotransferase levels	1261:1293	Serum biochemical analysis showed that dietary CHE decreased aspartate aminotransferase levels, while inclusion of IMO led to a decrease in high-density lipoprotein.					
35088457	13	27	theme	intestinal	2001:2010	arg1	composition					2022:2032	the intestinal microbial composition	1997:2032	the intestinal microbial composition of piglets	1997:2043	These results indicated that application of IMO or CHE could favourably modulate the intestinal microbial composition of piglets, while their beneficial impact and molecular mechanism on intestinal health warrants further investigation.					
35088457	10	28	theme	ileal	1601:1605	arg1	digesta					1607:1613	ileal digesta	1601:1613	ileal digesta	1601:1613	Further, CHE significantly increased the abundance of Bifidobacterium in ileal digesta.					
35088457	0	29	theme	intestinal	111:120	arg1	populations					132:142	intestinal bacterial populations	111:142	intestinal bacterial populations	111:142	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	10	30	theme	Bifidobacterium	1582:1596	arg1	abundance					1569:1577	the abundance	1565:1577	the abundance of Bifidobacterium in ileal digesta	1565:1613	Further, CHE significantly increased the abundance of Bifidobacterium in ileal digesta.					
35088457	7	31	theme	intestinal	1095:1104	arg1	morphology					1106:1115	intestinal morphology	1095:1115	intestinal morphology	1095:1115	Results showed that diets supplemented with IMO, CHE or their combination did not influence the diarrhoea rate and intestinal morphology, while co-administration of IMO with CHE tended to have higher average daily gain.					
35088457	1	32	theme	medicine	285:292	arg1	CHE					303:305	CHE	303:305	CHE	303:305	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	32	theme	medicine	285:292	arg1	extract					294:300	Chinese herbal medicine extract	270:300	Chinese herbal medicine extract (CHE)	270:306	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	12	33	theme	ileal	1858:1862	arg1	contents					1864:1871	ileal contents	1858:1871	ileal contents	1858:1871	Additionally, dietary IMO, CHE or their combination significantly reduced the number of potential entero-pathogen Escherichia coli in ileal contents and Clostridium species in caecal digesta.					
35088457	1	34	dep	performance	343:353	arg1	the					332:334	the	332:334	the	332:334	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	8	35	theme	IMO	1315:1317	arg1	inclusion					1302:1310	inclusion	1302:1310	inclusion of IMO	1302:1317	Serum biochemical analysis showed that dietary CHE decreased aspartate aminotransferase levels, while inclusion of IMO led to a decrease in high-density lipoprotein.					
35088457	0	36	theme	isomalto-oligosaccharides	11:35	arg1	extracts					48:55	isomalto-oligosaccharides and herbal extracts	11:55	isomalto-oligosaccharides and herbal extracts	11:55	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	9	37	theme	piglets	1519:1525	arg1	mucosa					1509:1514	jejunal mucosa	1501:1514	jejunal mucosa of piglets	1501:1525	Moreover, co-administration of IMO with CHE significantly upregulated the expression of TGF-β, a potent anti-inflammatory cytokine, in jejunal mucosa of piglets.					
35088457	5	38	with	diet	791:794	arg1	CHE					810:812	0.5 g/kg CHE	801:812	0.5 g/kg CHE	801:812	Group III were fed diet with 0.5 g/kg CHE for 14 days and 1 g/kg CHE for another 14 days.					
35088457	11	39	theme	IMO	1646:1648	arg1	Meanwhile					1616:1624	Meanwhile	1616:1624	Meanwhile	1616:1624	Meanwhile, the combination of IMO and CHE significantly increased Bifidobacterium in the caecum of piglets.					
35088457	11	39	theme	IMO	1646:1648	arg1	combination					1631:1641	the combination	1627:1641	the combination of IMO and CHE	1627:1656	Meanwhile, the combination of IMO and CHE significantly increased Bifidobacterium in the caecum of piglets.					
35088457	0	40	theme	herbal	41:46	arg1	extracts					48:55	isomalto-oligosaccharides and herbal extracts	11:55	isomalto-oligosaccharides and herbal extracts	11:55	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	12	41	theme	entero-pathogen	1822:1836	arg1	number					1802:1807	the number	1798:1807	the number of potential entero-pathogen Escherichia coli	1798:1853	Additionally, dietary IMO, CHE or their combination significantly reduced the number of potential entero-pathogen Escherichia coli in ileal contents and Clostridium species in caecal digesta.					
35088457	9	42	with	co-administration	1376:1392	arg1	CHE					1406:1408	CHE	1406:1408	CHE	1406:1408	Moreover, co-administration of IMO with CHE significantly upregulated the expression of TGF-β, a potent anti-inflammatory cytokine, in jejunal mucosa of piglets.					
35088457	8	43	theme	high-density	1340:1351	arg1	lipoprotein					1353:1363	high-density lipoprotein	1340:1363	high-density lipoprotein	1340:1363	Serum biochemical analysis showed that dietary CHE decreased aspartate aminotransferase levels, while inclusion of IMO led to a decrease in high-density lipoprotein.					
35088457	12	44	theme	dietary	1738:1744	arg1	IMO					1746:1748	dietary IMO	1738:1748	dietary IMO	1738:1748	Additionally, dietary IMO, CHE or their combination significantly reduced the number of potential entero-pathogen Escherichia coli in ileal contents and Clostridium species in caecal digesta.					
35088457	7	45	theme	daily	1188:1192	arg1	gain					1194:1197	higher average daily gain	1173:1197	higher average daily gain	1173:1197	Results showed that diets supplemented with IMO, CHE or their combination did not influence the diarrhoea rate and intestinal morphology, while co-administration of IMO with CHE tended to have higher average daily gain.					
35088457	11	46	theme	CHE	1654:1656	arg1	Meanwhile					1616:1624	Meanwhile	1616:1624	Meanwhile	1616:1624	Meanwhile, the combination of IMO and CHE significantly increased Bifidobacterium in the caecum of piglets.					
35088457	11	46	theme	CHE	1654:1656	arg1	combination					1631:1641	the combination	1627:1641	the combination of IMO and CHE	1627:1656	Meanwhile, the combination of IMO and CHE significantly increased Bifidobacterium in the caecum of piglets.					
35088457	13	47	theme	IMO	1960:1962	arg1	application					1945:1955	application	1945:1955	application of IMO or CHE	1945:1969	These results indicated that application of IMO or CHE could favourably modulate the intestinal microbial composition of piglets, while their beneficial impact and molecular mechanism on intestinal health warrants further investigation.					
35088457	7	48	theme	higher	1173:1178	arg1	gain					1194:1197	higher average daily gain	1173:1197	higher average daily gain	1173:1197	Results showed that diets supplemented with IMO, CHE or their combination did not influence the diarrhoea rate and intestinal morphology, while co-administration of IMO with CHE tended to have higher average daily gain.					
35088457	0	49	from	populations	132:142	arg1	piglets					160:166	early-weaned piglets	147:166	early-weaned piglets	147:166	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	13	50	theme	CHE	1967:1969	arg1	application					1945:1955	application	1945:1955	application of IMO or CHE	1945:1969	These results indicated that application of IMO or CHE could favourably modulate the intestinal microbial composition of piglets, while their beneficial impact and molecular mechanism on intestinal health warrants further investigation.					
35088457	12	51	dep	Escherichia	1838:1848	arg1	coli					1850:1853	coli	1850:1853	coli	1850:1853	Additionally, dietary IMO, CHE or their combination significantly reduced the number of potential entero-pathogen Escherichia coli in ileal contents and Clostridium species in caecal digesta.					
35088457	1	52	theme	Chinese	270:276	arg1	medicine					285:292	Chinese herbal medicine	270:292	Chinese herbal medicine extract (CHE)	270:306	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	13	53	from	mechanism	2090:2098	arg1	health					2114:2119	intestinal health	2103:2119	intestinal health	2103:2119	These results indicated that application of IMO or CHE could favourably modulate the intestinal microbial composition of piglets, while their beneficial impact and molecular mechanism on intestinal health warrants further investigation.					
35088457	10	54	from	abundance	1569:1577	arg1	digesta					1607:1613	ileal digesta	1601:1613	ileal digesta	1601:1613	Further, CHE significantly increased the abundance of Bifidobacterium in ileal digesta.					
35088457	9	55	from	expression	1440:1449	arg1	mucosa					1509:1514	jejunal mucosa	1501:1514	jejunal mucosa of piglets	1501:1525	Moreover, co-administration of IMO with CHE significantly upregulated the expression of TGF-β, a potent anti-inflammatory cytokine, in jejunal mucosa of piglets.					
35088457	7	56	theme	IMO	1145:1147	arg1	co-administration					1124:1140	co-administration	1124:1140	co-administration of IMO with CHE	1124:1156	Results showed that diets supplemented with IMO, CHE or their combination did not influence the diarrhoea rate and intestinal morphology, while co-administration of IMO with CHE tended to have higher average daily gain.					
35088457	1	57	theme	weaned	479:484	arg1	piglets					486:492	weaned piglets	479:492	weaned piglets	479:492	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	58	theme	study	198:202	arg1	objective					173:181	The objective	169:181	The objective of the current study	169:202	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	9	59	theme	IMO	1397:1399	arg1	co-administration					1376:1392	co-administration	1376:1392	co-administration of IMO with CHE	1376:1408	Moreover, co-administration of IMO with CHE significantly upregulated the expression of TGF-β, a potent anti-inflammatory cytokine, in jejunal mucosa of piglets.					
35088457	6	60	theme	IMO + 0.5 g/kg	898:911	arg1	CHE					913:915	2 g/kg IMO + 0.5 g/kg CHE	891:915	2 g/kg IMO + 0.5 g/kg CHE	891:915	Group IV were fed diet with (2 g/kg IMO + 0.5 g/kg CHE) for 14 days and (4 g/kg IMO +1 g/kg CHE) for another 14 days.					
35088457	6	60	theme	IMO + 0.5 g/kg	898:911	arg1	diet					880:883	diet	880:883	diet with (2 g/kg IMO + 0.5 g/kg CHE) for 14 days and (4 g/kg IMO +1 g/kg CHE)	880:957	Group IV were fed diet with (2 g/kg IMO + 0.5 g/kg CHE) for 14 days and (4 g/kg IMO +1 g/kg CHE) for another 14 days.					
35088457	1	61	theme	biochemical	383:393	arg1	profiles					395:402	serum biochemical profiles	377:402	serum biochemical profiles	377:402	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	5	62	theme	0.5 g/kg	801:808	arg1	CHE					810:812	0.5 g/kg CHE	801:812	0.5 g/kg CHE	801:812	Group III were fed diet with 0.5 g/kg CHE for 14 days and 1 g/kg CHE for another 14 days.					
35088457	12	63	theme	caecal	1900:1905	arg1	digesta					1907:1913	caecal digesta	1900:1913	caecal digesta	1900:1913	Additionally, dietary IMO, CHE or their combination significantly reduced the number of potential entero-pathogen Escherichia coli in ileal contents and Clostridium species in caecal digesta.					
35088457	1	64	from	effects	227:233	arg1	profiles					395:402	serum biochemical profiles	377:402	serum biochemical profiles	377:402	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	64	from	effects	227:233	arg1	morphology					450:459	intestinal morphology	439:459	intestinal morphology	439:459	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	64	from	effects	227:233	arg1	microflora					465:474	microflora	465:474	microflora	465:474	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	64	from	effects	227:233	arg1	incidence					366:374	diarrhoea incidence	356:374	diarrhoea incidence	356:374	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	64	from	effects	227:233	arg1	expression					427:436	inflammatory cytokine expression	405:436	inflammatory cytokine expression	405:436	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	1	64	from	effects	227:233	arg1	performance					343:353	growth performance	336:353	growth performance	336:353	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	7	65	with	co-administration	1124:1140	arg1	CHE					1154:1156	CHE	1154:1156	CHE	1154:1156	Results showed that diets supplemented with IMO, CHE or their combination did not influence the diarrhoea rate and intestinal morphology, while co-administration of IMO with CHE tended to have higher average daily gain.					
35088457	8	66	theme	Serum	1200:1204	arg1	analysis					1218:1225	Serum biochemical analysis	1200:1225	Serum biochemical analysis	1200:1225	Serum biochemical analysis showed that dietary CHE decreased aspartate aminotransferase levels, while inclusion of IMO led to a decrease in high-density lipoprotein.					
35088457	1	67	theme	cytokine	418:425	arg1	expression					427:436	inflammatory cytokine expression	405:436	inflammatory cytokine expression	405:436	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	2	68	theme	age	569:571	arg1	25 days					558:564	25 days	558:564	25 days of age	558:571	Thirty-two ([Landrace × Yorkshire] × Duroc) piglets, weaned at 25 days of age, were randomly assigned into four groups.					
35088457	4	69	theme	2 g/kg	696:701	arg1	IMO					703:705	2 g/kg IMO	696:705	2 g/kg IMO	696:705	Group II were fed a basal diet supplemented with 2 g/kg IMO for 14 consecutive days and then 4 g/kg IMO for another 14 days.					
35088457	13	70	theme	molecular	2080:2088	arg1	mechanism					2090:2098	molecular mechanism	2080:2098	molecular mechanism	2080:2098	These results indicated that application of IMO or CHE could favourably modulate the intestinal microbial composition of piglets, while their beneficial impact and molecular mechanism on intestinal health warrants further investigation.					
35088457	9	71	theme	TGF-β	1454:1458	arg1	expression					1440:1449	the expression	1436:1449	the expression of TGF-β, a potent anti-inflammatory cytokine, in jejunal mucosa of piglets	1436:1525	Moreover, co-administration of IMO with CHE significantly upregulated the expression of TGF-β, a potent anti-inflammatory cytokine, in jejunal mucosa of piglets.					
35088457	6	72	theme	IMO +1 g/kg	942:952	arg1	CHE					954:956	4 g/kg IMO +1 g/kg CHE	935:956	(4 g/kg IMO +1 g/kg CHE)	934:957	Group IV were fed diet with (2 g/kg IMO + 0.5 g/kg CHE) for 14 days and (4 g/kg IMO +1 g/kg CHE) for another 14 days.					
35088457	2	73	theme	[Landrace × Yorkshire	507:527	arg1	piglets					539:545	Thirty-two ([Landrace × Yorkshire] × Duroc) piglets	495:545	Thirty-two ([Landrace × Yorkshire] × Duroc) piglets	495:545	Thirty-two ([Landrace × Yorkshire] × Duroc) piglets, weaned at 25 days of age, were randomly assigned into four groups.					
35088457	0	74	theme	biochemical	86:96	arg1	profiles					98:105	serum biochemical profiles	80:105	serum biochemical profiles	80:105	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	12	75	theme	Clostridium	1877:1887	arg1	species					1889:1895	Clostridium species	1877:1895	Clostridium species	1877:1895	Additionally, dietary IMO, CHE or their combination significantly reduced the number of potential entero-pathogen Escherichia coli in ileal contents and Clostridium species in caecal digesta.					
35088457	12	76	from	contents	1864:1871	arg1	digesta					1907:1913	caecal digesta	1900:1913	caecal digesta	1900:1913	Additionally, dietary IMO, CHE or their combination significantly reduced the number of potential entero-pathogen Escherichia coli in ileal contents and Clostridium species in caecal digesta.					
35088457	2	77	theme	Thirty-two	495:504	arg1	piglets					539:545	Thirty-two ([Landrace × Yorkshire] × Duroc) piglets	495:545	Thirty-two ([Landrace × Yorkshire] × Duroc) piglets	495:545	Thirty-two ([Landrace × Yorkshire] × Duroc) piglets, weaned at 25 days of age, were randomly assigned into four groups.					
35088457	4	78	theme	basal	667:671	arg1	diet					673:676	a basal diet	665:676	a basal diet supplemented with 2 g/kg IMO	665:705	Group II were fed a basal diet supplemented with 2 g/kg IMO for 14 consecutive days and then 4 g/kg IMO for another 14 days.					
35088457	7	79	contain	have	1168:1171	arg2	gain					1194:1197	higher average daily gain	1173:1197	higher average daily gain	1173:1197	Results showed that diets supplemented with IMO, CHE or their combination did not influence the diarrhoea rate and intestinal morphology, while co-administration of IMO with CHE tended to have higher average daily gain.					
35088457	7	79	contain	have	1168:1171	arg1	co-administration					1124:1140	co-administration	1124:1140	co-administration of IMO with CHE	1124:1156	Results showed that diets supplemented with IMO, CHE or their combination did not influence the diarrhoea rate and intestinal morphology, while co-administration of IMO with CHE tended to have higher average daily gain.					
35088457	9	80	theme	anti-inflammatory	1470:1486	arg1	cytokine					1488:1495	a potent anti-inflammatory cytokine	1461:1495	a potent anti-inflammatory cytokine	1461:1495	Moreover, co-administration of IMO with CHE significantly upregulated the expression of TGF-β, a potent anti-inflammatory cytokine, in jejunal mucosa of piglets.					
35088457	9	80	theme	anti-inflammatory	1470:1486	arg1	TGF-β					1454:1458	TGF-β	1454:1458	TGF-β	1454:1458	Moreover, co-administration of IMO with CHE significantly upregulated the expression of TGF-β, a potent anti-inflammatory cytokine, in jejunal mucosa of piglets.					
35088457	0	81	from	profiles	98:105	arg1	piglets					160:166	early-weaned piglets	147:166	early-weaned piglets	147:166	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	8	82	theme	aspartate	1261:1269	arg1	levels					1288:1293	aspartate aminotransferase levels	1261:1293	aspartate aminotransferase levels	1261:1293	Serum biochemical analysis showed that dietary CHE decreased aspartate aminotransferase levels, while inclusion of IMO led to a decrease in high-density lipoprotein.					
35088457	0	83	theme	bacterial	122:130	arg1	populations					132:142	intestinal bacterial populations	111:142	intestinal bacterial populations	111:142	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	1	84	theme	diarrhoea	356:364	arg1	incidence					366:374	diarrhoea incidence	356:374	diarrhoea incidence	356:374	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	2	85	theme	 × Duroc	529:536	arg1	piglets					539:545	Thirty-two ([Landrace × Yorkshire] × Duroc) piglets	495:545	Thirty-two ([Landrace × Yorkshire] × Duroc) piglets	495:545	Thirty-two ([Landrace × Yorkshire] × Duroc) piglets, weaned at 25 days of age, were randomly assigned into four groups.					
35088457	4	86	theme	4 g/kg	740:745	arg1	IMO					747:749	4 g/kg IMO	740:749	4 g/kg IMO for another 14 days	740:769	Group II were fed a basal diet supplemented with 2 g/kg IMO for 14 consecutive days and then 4 g/kg IMO for another 14 days.					
35088457	9	87	theme	jejunal	1501:1507	arg1	mucosa					1509:1514	jejunal mucosa	1501:1514	jejunal mucosa of piglets	1501:1525	Moreover, co-administration of IMO with CHE significantly upregulated the expression of TGF-β, a potent anti-inflammatory cytokine, in jejunal mucosa of piglets.					
35088457	13	88	theme	microbial	2012:2020	arg1	composition					2022:2032	the intestinal microbial composition	1997:2032	the intestinal microbial composition of piglets	1997:2043	These results indicated that application of IMO or CHE could favourably modulate the intestinal microbial composition of piglets, while their beneficial impact and molecular mechanism on intestinal health warrants further investigation.					
35088457	7	89	theme	diarrhoea	1076:1084	arg1	rate					1086:1089	the diarrhoea rate	1072:1089	the diarrhoea rate	1072:1089	Results showed that diets supplemented with IMO, CHE or their combination did not influence the diarrhoea rate and intestinal morphology, while co-administration of IMO with CHE tended to have higher average daily gain.					
35088457	1	90	theme	extract	294:300	arg1	effects					227:233	the effects	223:233	the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets	223:492	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	12	91	theme	potential	1812:1820	arg1	entero-pathogen					1822:1836	potential entero-pathogen	1812:1836	potential entero-pathogen Escherichia coli	1812:1853	Additionally, dietary IMO, CHE or their combination significantly reduced the number of potential entero-pathogen Escherichia coli in ileal contents and Clostridium species in caecal digesta.					
35088457	3	92	theme	basal	635:639	arg1	diet					641:644	the basal diet	631:644	the basal diet	631:644	Group I was fed the basal diet.					
35088457	4	93	theme	consecutive	714:724	arg1	days					726:729	14 consecutive days	711:729	14 consecutive days	711:729	Group II were fed a basal diet supplemented with 2 g/kg IMO for 14 consecutive days and then 4 g/kg IMO for another 14 days.					
35088457	0	94	theme	extracts	48:55	arg1	Effects					0:6	Effects	0:6	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.	0:167	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	12	95	theme	Escherichia	1838:1848	arg1	entero-pathogen					1822:1836	potential entero-pathogen	1812:1836	potential entero-pathogen Escherichia coli	1812:1853	Additionally, dietary IMO, CHE or their combination significantly reduced the number of potential entero-pathogen Escherichia coli in ileal contents and Clostridium species in caecal digesta.					
35088457	0	96	theme	growth	60:65	arg1	performance					67:77	growth performance	60:77	growth performance	60:77	Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned piglets.					
35088457	1	97	theme	combination	317:327	arg1	effects					227:233	the effects	223:233	the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets	223:492	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
35088457	7	98	theme	average	1180:1186	arg1	gain					1194:1197	higher average daily gain	1173:1197	higher average daily gain	1173:1197	Results showed that diets supplemented with IMO, CHE or their combination did not influence the diarrhoea rate and intestinal morphology, while co-administration of IMO with CHE tended to have higher average daily gain.					
35088457	1	99	theme	isomalto-oligosaccharide	238:261	arg1	effects					227:233	the effects	223:233	the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets	223:492	The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets.					
36502627	5	0	theme	good	793:796	arg1	compatibility					803:815	good cell compatibility	793:815	good cell compatibility	793:815	The co-culture of L929 cells and the CCK-8 experiments demonstrated good cell compatibility and low scaffold cytotoxicity, respectively.					
36502627	8	1	theme	moisture	1212:1219	arg1	retention					1221:1229	high moisture retention	1207:1229	high moisture retention	1207:1229	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	2	2	theme	antibacterial	372:384	arg1	properties					386:395	antibacterial properties	372:395	antibacterial properties	372:395	The new skin tissue engineering scaffold provides innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties.					
36502627	5	3	theme	CCK-8	762:766	arg1	co-culture					729:738	The co-culture	725:738	The co-culture of L929 cells and the CCK-8 experiments	725:778	The co-culture of L929 cells and the CCK-8 experiments demonstrated good cell compatibility and low scaffold cytotoxicity, respectively.					
36502627	5	4	theme	cell	798:801	arg1	compatibility					803:815	good cell compatibility	793:815	good cell compatibility	793:815	The co-culture of L929 cells and the CCK-8 experiments demonstrated good cell compatibility and low scaffold cytotoxicity, respectively.					
36502627	7	5	theme	SSF/CS/HAP	1005:1014	arg1	group					1025:1029	the SSF/CS/HAP scaffold group	1001:1029	the SSF/CS/HAP scaffold group	1001:1029	In the wound skin tissue of the SSF/CS/HAP scaffold group, immunohistochemistry indicates a more rapid and mature development of hair follicles.					
36502627	6	6	dep	heal	960:963	arg1	faster					965:970	faster	965:970	faster	965:970	Meanwhile, in vivo experiments demonstrate that rats with SSF/CS/HAP scaffold-treated neck wounds heal faster.					
36502627	5	7	theme	L929 cells	743:752	arg1	co-culture					729:738	The co-culture	725:738	The co-culture of L929 cells and the CCK-8 experiments	725:778	The co-culture of L929 cells and the CCK-8 experiments demonstrated good cell compatibility and low scaffold cytotoxicity, respectively.					
36502627	6	8	theme	heal	960:963	arg1	wounds					953:958	neck wounds	948:958	neck wounds heal faster	948:970	Meanwhile, in vivo experiments demonstrate that rats with SSF/CS/HAP scaffold-treated neck wounds heal faster.					
36502627	7	9	theme	scaffold	1016:1023	arg1	group					1025:1029	the SSF/CS/HAP scaffold group	1001:1029	the SSF/CS/HAP scaffold group	1001:1029	In the wound skin tissue of the SSF/CS/HAP scaffold group, immunohistochemistry indicates a more rapid and mature development of hair follicles.					
36502627	2	10	theme	success	297:303	arg1	chance					287:292	a good chance	280:292	a good chance of success	280:303	The new skin tissue engineering scaffold provides innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties.					
36502627	8	11	theme	tissue	1237:1242	arg1	compatibility					1244:1256	high tissue compatibility	1232:1256	high tissue compatibility	1232:1256	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	6	12	theme	neck	948:951	arg1	wounds					953:958	neck wounds	948:958	neck wounds heal faster	948:970	Meanwhile, in vivo experiments demonstrate that rats with SSF/CS/HAP scaffold-treated neck wounds heal faster.					
36502627	3	13	dep	fabrication	422:432	arg1	the					418:420	the	418:420	the	418:420	This paper presents the fabrication and evaluation of a three-dimensional composite scaffold made with sulfated silk fibroin, chitosan, and hydroxyapatite (SSF/CS/HAP).					
36502627	8	14	theme	wound	1318:1322	arg1	repair					1324:1329	wound repair	1318:1329	wound repair	1318:1329	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	3	15	theme	composite	472:480	arg1	scaffold					482:489	a three-dimensional composite scaffold	452:489	a three-dimensional composite scaffold made with sulfated silk fibroin, chitosan, and hydroxyapatite (SSF/CS/HAP)	452:564	This paper presents the fabrication and evaluation of a three-dimensional composite scaffold made with sulfated silk fibroin, chitosan, and hydroxyapatite (SSF/CS/HAP).					
36502627	5	16	theme	scaffold	825:832	arg1	cytotoxicity					834:845	low scaffold cytotoxicity	821:845	low scaffold cytotoxicity	821:845	The co-culture of L929 cells and the CCK-8 experiments demonstrated good cell compatibility and low scaffold cytotoxicity, respectively.					
36502627	7	17	theme	skin	986:989	arg1	tissue					991:996	the wound skin tissue	976:996	the wound skin tissue of the SSF/CS/HAP scaffold group	976:1029	In the wound skin tissue of the SSF/CS/HAP scaffold group, immunohistochemistry indicates a more rapid and mature development of hair follicles.					
36502627	8	18	dep	engineering	1172:1182	arg1	material					1193:1200	scaffold material	1184:1200	a novel skin tissue engineering scaffold material	1152:1200	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	8	19	theme	novel	1154:1158	arg1	engineering					1172:1182	a novel skin tissue engineering	1152:1182	a novel skin tissue engineering scaffold material	1152:1200	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	3	20	theme	scaffold	482:489	arg1	evaluation					438:447	evaluation	438:447	evaluation	438:447	This paper presents the fabrication and evaluation of a three-dimensional composite scaffold made with sulfated silk fibroin, chitosan, and hydroxyapatite (SSF/CS/HAP).					
36502627	3	20	theme	scaffold	482:489	arg1	fabrication					422:432	fabrication	422:432	fabrication	422:432	This paper presents the fabrication and evaluation of a three-dimensional composite scaffold made with sulfated silk fibroin, chitosan, and hydroxyapatite (SSF/CS/HAP).					
36502627	0	21	theme	skin	4:7	arg1	tissue					9:14	New skin tissue	0:14	New skin tissue	0:14	New skin tissue engineering scaffold with sulfated silk fibroin/chitosan/hydroxyapatite and its application.					
36502627	8	22	theme	high	1207:1210	arg1	retention					1221:1229	high moisture retention	1207:1229	high moisture retention	1207:1229	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	2	23	theme	mechanical	327:336	arg1	properties					338:347	its stable mechanical properties	316:347	its stable mechanical properties	316:347	The new skin tissue engineering scaffold provides innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties.					
36502627	8	24	theme	skin	1160:1163	arg1	engineering					1172:1182	a novel skin tissue engineering	1152:1182	a novel skin tissue engineering scaffold material	1152:1200	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	4	25	theme	expansion	695:703	arg1	index					705:709	its expansion index	691:709	its expansion index	691:709	An electron microscope shows that the scaffold has an aperture of 15-20 μm, while an absorption performance test shows that its expansion index reaches 779%.					
36502627	0	26	theme	New	0:2	arg1	tissue					9:14	New skin tissue	0:14	New skin tissue	0:14	New skin tissue engineering scaffold with sulfated silk fibroin/chitosan/hydroxyapatite and its application.					
36502627	8	27	theme	high	1232:1235	arg1	compatibility					1244:1256	high tissue compatibility	1232:1256	high tissue compatibility	1232:1256	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	6	28	theme	Meanwhile	862:870	arg1	experiments					881:891	Meanwhile, in vivo experiments	862:891	Meanwhile, in vivo experiments	862:891	Meanwhile, in vivo experiments demonstrate that rats with SSF/CS/HAP scaffold-treated neck wounds heal faster.					
36502627	2	29	theme	stable	320:325	arg1	properties					338:347	its stable mechanical properties	316:347	its stable mechanical properties	316:347	The new skin tissue engineering scaffold provides innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties.					
36502627	3	30	theme	three-dimensional	454:470	arg1	scaffold					482:489	a three-dimensional composite scaffold	452:489	a three-dimensional composite scaffold made with sulfated silk fibroin, chitosan, and hydroxyapatite (SSF/CS/HAP)	452:564	This paper presents the fabrication and evaluation of a three-dimensional composite scaffold made with sulfated silk fibroin, chitosan, and hydroxyapatite (SSF/CS/HAP).					
36502627	8	31	theme	low	1263:1265	arg1	cytotoxicity					1267:1278	low cytotoxicity	1263:1278	low cytotoxicity	1263:1278	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	6	32	dep	Meanwhile	862:870	arg1	in vivo					873:879	in vivo	873:879	in vivo	873:879	Meanwhile, in vivo experiments demonstrate that rats with SSF/CS/HAP scaffold-treated neck wounds heal faster.					
36502627	7	33	theme	wound	980:984	arg1	tissue					991:996	the wound skin tissue	976:996	the wound skin tissue of the SSF/CS/HAP scaffold group	976:1029	In the wound skin tissue of the SSF/CS/HAP scaffold group, immunohistochemistry indicates a more rapid and mature development of hair follicles.					
36502627	1	34	theme	skin	119:122	arg1	wounds					124:129	skin wounds	119:129	skin wounds	119:129	Repairing skin wounds has always been challenging in clinical practice.					
36502627	3	35	theme	sulfated	501:508	arg1	fibroin					515:521	sulfated silk fibroin	501:521	sulfated silk fibroin	501:521	This paper presents the fabrication and evaluation of a three-dimensional composite scaffold made with sulfated silk fibroin, chitosan, and hydroxyapatite (SSF/CS/HAP).					
36502627	2	36	theme	tissue	194:199	arg1	scaffold					213:220	The new skin tissue engineering scaffold	181:220	The new skin tissue engineering scaffold	181:220	The new skin tissue engineering scaffold provides innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties.					
36502627	3	37	theme	silk	510:513	arg1	fibroin					515:521	sulfated silk fibroin	501:521	sulfated silk fibroin	501:521	This paper presents the fabrication and evaluation of a three-dimensional composite scaffold made with sulfated silk fibroin, chitosan, and hydroxyapatite (SSF/CS/HAP).					
36502627	7	38	theme	follicles	1107:1115	arg1	development					1087:1097	a more rapid and mature development	1063:1097	a more rapid and mature development of hair follicles	1063:1115	In the wound skin tissue of the SSF/CS/HAP scaffold group, immunohistochemistry indicates a more rapid and mature development of hair follicles.					
36502627	2	39	theme	skin	189:192	arg1	scaffold					213:220	The new skin tissue engineering scaffold	181:220	The new skin tissue engineering scaffold	181:220	The new skin tissue engineering scaffold provides innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties.					
36502627	6	40	with	rats	910:913	arg1	SSF/CS/HAP					920:929	SSF/CS/HAP	920:929	SSF/CS/HAP	920:929	Meanwhile, in vivo experiments demonstrate that rats with SSF/CS/HAP scaffold-treated neck wounds heal faster.					
36502627	8	41	theme	scaffold	1184:1191	arg1	material					1193:1200	scaffold material	1184:1200	a novel skin tissue engineering scaffold material	1152:1200	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	2	42	theme	new	185:187	arg1	scaffold					213:220	The new skin tissue engineering scaffold	181:220	The new skin tissue engineering scaffold	181:220	The new skin tissue engineering scaffold provides innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties.					
36502627	7	43	theme	rapid	1070:1074	arg1	development					1087:1097	a more rapid and mature development	1063:1097	a more rapid and mature development of hair follicles	1063:1115	In the wound skin tissue of the SSF/CS/HAP scaffold group, immunohistochemistry indicates a more rapid and mature development of hair follicles.					
36502627	4	44	theme	performance	663:673	arg1	test					675:678	an absorption performance test	649:678	an absorption performance test	649:678	An electron microscope shows that the scaffold has an aperture of 15-20 μm, while an absorption performance test shows that its expansion index reaches 779%.					
36502627	0	45	theme	silk	51:54	arg1	fibroin/chitosan/hydroxyapatite					56:86	sulfated silk fibroin/chitosan/hydroxyapatite	42:86	sulfated silk fibroin/chitosan/hydroxyapatite	42:86	New skin tissue engineering scaffold with sulfated silk fibroin/chitosan/hydroxyapatite and its application.					
36502627	2	46	with	challenges	264:273	arg1	chance					287:292	a good chance	280:292	a good chance of success	280:303	The new skin tissue engineering scaffold provides innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties.					
36502627	8	47	theme	tissue	1165:1170	arg1	engineering					1172:1182	a novel skin tissue engineering	1152:1182	a novel skin tissue engineering scaffold material	1152:1200	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	7	48	theme	mature	1080:1085	arg1	development					1087:1097	a more rapid and mature development	1063:1097	a more rapid and mature development of hair follicles	1063:1115	In the wound skin tissue of the SSF/CS/HAP scaffold group, immunohistochemistry indicates a more rapid and mature development of hair follicles.					
36502627	8	49	theme	tissue	1360:1365	arg1	applications					1379:1390	tissue engineering applications	1360:1390	tissue engineering applications	1360:1390	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	0	50	theme	sulfated	42:49	arg1	fibroin/chitosan/hydroxyapatite					56:86	sulfated silk fibroin/chitosan/hydroxyapatite	42:86	sulfated silk fibroin/chitosan/hydroxyapatite	42:86	New skin tissue engineering scaffold with sulfated silk fibroin/chitosan/hydroxyapatite and its application.					
36502627	4	51	theme	electron	570:577	arg1	microscope					579:588	An electron microscope	567:588	An electron microscope	567:588	An electron microscope shows that the scaffold has an aperture of 15-20 μm, while an absorption performance test shows that its expansion index reaches 779%.					
36502627	7	52	theme	hair	1102:1105	arg1	follicles					1107:1115	hair follicles	1102:1115	hair follicles	1102:1115	In the wound skin tissue of the SSF/CS/HAP scaffold group, immunohistochemistry indicates a more rapid and mature development of hair follicles.					
36502627	2	53	theme	innovative	231:240	arg1	ways					242:245	innovative ways	231:245	innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties	231:395	The new skin tissue engineering scaffold provides innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties.					
36502627	4	54	contain	has	614:616	arg1	scaffold					605:612	the scaffold	601:612	the scaffold	601:612	An electron microscope shows that the scaffold has an aperture of 15-20 μm, while an absorption performance test shows that its expansion index reaches 779%.					
36502627	4	54	contain	has	614:616	arg2	aperture					621:628	an aperture	618:628	an aperture of 15-20 μm	618:640	An electron microscope shows that the scaffold has an aperture of 15-20 μm, while an absorption performance test shows that its expansion index reaches 779%.					
36502627	5	55	dep	L929 cells	743:752	arg1	experiments					768:778	experiments	768:778	experiments	768:778	The co-culture of L929 cells and the CCK-8 experiments demonstrated good cell compatibility and low scaffold cytotoxicity, respectively.					
36502627	2	56	theme	good	282:285	arg1	chance					287:292	a good chance	280:292	a good chance of success	280:303	The new skin tissue engineering scaffold provides innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties.					
36502627	4	57	theme	15-20 μm	633:640	arg1	aperture					621:628	an aperture	618:628	an aperture of 15-20 μm	618:640	An electron microscope shows that the scaffold has an aperture of 15-20 μm, while an absorption performance test shows that its expansion index reaches 779%.					
36502627	8	58	theme	engineering	1367:1377	arg1	applications					1379:1390	tissue engineering applications	1360:1390	tissue engineering applications	1360:1390	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	2	59	theme	engineering	201:211	arg1	scaffold					213:220	The new skin tissue engineering scaffold	181:220	The new skin tissue engineering scaffold	181:220	The new skin tissue engineering scaffold provides innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties.					
36502627	8	60	theme	promising	1336:1344	arg1	potential					1346:1354	promising potential	1336:1354	promising potential for tissue engineering applications	1336:1390	This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.					
36502627	4	61	theme	absorption	652:661	arg1	test					675:678	an absorption performance test	649:678	an absorption performance test	649:678	An electron microscope shows that the scaffold has an aperture of 15-20 μm, while an absorption performance test shows that its expansion index reaches 779%.					
36502627	5	62	theme	low	821:823	arg1	cytotoxicity					834:845	low scaffold cytotoxicity	821:845	low scaffold cytotoxicity	821:845	The co-culture of L929 cells and the CCK-8 experiments demonstrated good cell compatibility and low scaffold cytotoxicity, respectively.					
36502627	7	63	theme	group	1025:1029	arg1	tissue					991:996	the wound skin tissue	976:996	the wound skin tissue of the SSF/CS/HAP scaffold group	976:1029	In the wound skin tissue of the SSF/CS/HAP scaffold group, immunohistochemistry indicates a more rapid and mature development of hair follicles.					
36502627	1	64	theme	clinical	162:169	arg1	practice					171:178	clinical practice	162:178	clinical practice	162:178	Repairing skin wounds has always been challenging in clinical practice.					
37054511	0	0	theme	films	86:90	arg1	Preparation					0:10	Preparation	0:10	Preparation of robust, water-resistant, antibacterial, and antioxidant chitosan-based films by incorporation of cinnamaldehyde-tannin acid-zinc acetate	0:150	Preparation of robust, water-resistant, antibacterial, and antioxidant chitosan-based films by incorporation of cinnamaldehyde-tannin acid-zinc acetate nanoparticles.					
37054511	5	1	theme	UV	810:811	arg1	adsorption					813:822	the UV adsorption	806:822	the UV adsorption	806:822	Furthermore, CTZA NPs significantly enhanced the UV adsorption, antibacterial, and antioxidant properties of the films, while reduced their water vapor permeability.					
37054511	3	2	theme	contact	548:554	arg1	angle					556:560	water contact angle	542:560	water contact angle	542:560	The tensile strength and water contact angle of the composite films increased about 5.25-fold and 17.55°.					
37054511	6	3	theme	NPs	1027:1029	arg1	presence					994:1001	the presence	990:1001	the presence of the hydrophobic CTZA NPs	990:1029	Moreover, it was possible to print inks onto the films because the presence of the hydrophobic CTZA NPs facilitated the deposition of carbon powder onto their surfaces.					
37054511	0	4	theme	chitosan-based	71:84	arg1	films					86:90	robust, water-resistant, antibacterial, and antioxidant chitosan-based films	15:90	robust, water-resistant, antibacterial, and antioxidant chitosan-based films	15:90	Preparation of robust, water-resistant, antibacterial, and antioxidant chitosan-based films by incorporation of cinnamaldehyde-tannin acid-zinc acetate nanoparticles.					
37054511	6	5	theme	CTZA	1022:1025	arg1	NPs					1027:1029	the hydrophobic CTZA NPs	1006:1029	the hydrophobic CTZA NPs	1006:1029	Moreover, it was possible to print inks onto the films because the presence of the hydrophobic CTZA NPs facilitated the deposition of carbon powder onto their surfaces.					
37054511	7	6	theme	antioxidant	1135:1145	arg1	activities					1147:1156	great antibacterial and antioxidant activities	1111:1156	great antibacterial and antioxidant activities	1111:1156	The films with great antibacterial and antioxidant activities can be applied for food packaging application.					
37054511	4	7	theme	CTZA	639:642	arg1	NPs					644:646	CTZA NPs	639:646	CTZA NPs	639:646	The addition of CTZA NPs reduced the water sensitivity of CS films, which could undergo appreciable stretching in water without breaking.					
37054511	5	8	theme	CTZA	774:777	arg1	NPs					779:781	CTZA NPs	774:781	CTZA NPs	774:781	Furthermore, CTZA NPs significantly enhanced the UV adsorption, antibacterial, and antioxidant properties of the films, while reduced their water vapor permeability.					
37054511	3	9	dep	strength	529:536	arg1	The					517:519	The	517:519	The	517:519	The tensile strength and water contact angle of the composite films increased about 5.25-fold and 17.55°.					
37054511	0	10	theme	cinnamaldehyde-tannin	112:132	arg1	acetate					144:150	cinnamaldehyde-tannin acid-zinc acetate	112:150	cinnamaldehyde-tannin acid-zinc acetate	112:150	Preparation of robust, water-resistant, antibacterial, and antioxidant chitosan-based films by incorporation of cinnamaldehyde-tannin acid-zinc acetate nanoparticles.					
37054511	7	11	theme	antibacterial	1117:1129	arg1	activities					1147:1156	great antibacterial and antioxidant activities	1111:1156	great antibacterial and antioxidant activities	1111:1156	The films with great antibacterial and antioxidant activities can be applied for food packaging application.					
37054511	3	12	theme	composite	569:577	arg1	films					579:583	the composite films	565:583	the composite films	565:583	The tensile strength and water contact angle of the composite films increased about 5.25-fold and 17.55°.					
37054511	2	13	theme	medicinal	424:432	arg1	extracts					440:447	edible medicinal plant extracts	417:447	edible medicinal plant extracts	417:447	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts were successfully incorporated into CS films to solve these issues.					
37054511	3	14	theme	films	579:583	arg1	angle					556:560	water contact angle	542:560	water contact angle	542:560	The tensile strength and water contact angle of the composite films increased about 5.25-fold and 17.55°.					
37054511	3	14	theme	films	579:583	arg1	strength					529:536	tensile strength	521:536	tensile strength	521:536	The tensile strength and water contact angle of the composite films increased about 5.25-fold and 17.55°.					
37054511	2	15	theme	plant	434:438	arg1	extracts					440:447	edible medicinal plant extracts	417:447	edible medicinal plant extracts	417:447	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts were successfully incorporated into CS films to solve these issues.					
37054511	5	16	theme	antioxidant	844:854	arg1	properties					856:865	antioxidant properties	844:865	antioxidant properties of the films	844:878	Furthermore, CTZA NPs significantly enhanced the UV adsorption, antibacterial, and antioxidant properties of the films, while reduced their water vapor permeability.					
37054511	1	17	from	application	294:304	arg1	industry					327:334	food preservation industry	309:334	food preservation industry	309:334	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	2	18	theme	acid-zinc	359:367	arg1	nanoparticles					377:389	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles	337:389	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts	337:447	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts were successfully incorporated into CS films to solve these issues.					
37054511	2	18	theme	acid-zinc	359:367	arg1	NPs					397:399	CTZA NPs	392:399	CTZA NPs	392:399	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts were successfully incorporated into CS films to solve these issues.					
37054511	1	19	theme	poor	192:195	arg1	activity					265:272	limited antimicrobial activity	243:272	limited antimicrobial activity	243:272	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	1	19	theme	poor	192:195	arg1	property					208:215	poor mechanical property	192:215	poor mechanical property	192:215	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	1	19	theme	poor	192:195	arg1	water-resistance					222:237	low water-resistance	218:237	low water-resistance	218:237	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	7	20	theme	packaging	1182:1190	arg1	application					1192:1202	food packaging application	1177:1202	food packaging application	1177:1202	The films with great antibacterial and antioxidant activities can be applied for food packaging application.					
37054511	7	21	theme	great	1111:1115	arg1	activities					1147:1156	great antibacterial and antioxidant activities	1111:1156	great antibacterial and antioxidant activities	1111:1156	The films with great antibacterial and antioxidant activities can be applied for food packaging application.					
37054511	2	22	theme	Cinnamaldehyde-tannic	337:357	arg1	nanoparticles					377:389	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles	337:389	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts	337:447	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts were successfully incorporated into CS films to solve these issues.					
37054511	2	22	theme	Cinnamaldehyde-tannic	337:357	arg1	NPs					397:399	CTZA NPs	392:399	CTZA NPs	392:399	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts were successfully incorporated into CS films to solve these issues.					
37054511	1	23	theme	mechanical	197:206	arg1	activity					265:272	limited antimicrobial activity	243:272	limited antimicrobial activity	243:272	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	1	23	theme	mechanical	197:206	arg1	property					208:215	poor mechanical property	192:215	poor mechanical property	192:215	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	1	23	theme	mechanical	197:206	arg1	water-resistance					222:237	low water-resistance	218:237	low water-resistance	218:237	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	4	24	theme	NPs	644:646	arg1	addition					627:634	The addition	623:634	The addition of CTZA NPs	623:646	The addition of CTZA NPs reduced the water sensitivity of CS films, which could undergo appreciable stretching in water without breaking.					
37054511	0	25	theme	acetate	144:150	arg1	incorporation					95:107	incorporation	95:107	incorporation of cinnamaldehyde-tannin acid-zinc acetate	95:150	Preparation of robust, water-resistant, antibacterial, and antioxidant chitosan-based films by incorporation of cinnamaldehyde-tannin acid-zinc acetate nanoparticles.					
37054511	6	26	theme	powder	1068:1073	arg1	deposition					1047:1056	the deposition	1043:1056	the deposition of carbon powder onto their surfaces	1043:1093	Moreover, it was possible to print inks onto the films because the presence of the hydrophobic CTZA NPs facilitated the deposition of carbon powder onto their surfaces.					
37054511	5	27	theme	films	874:878	arg1	properties					856:865	antioxidant properties	844:865	antioxidant properties of the films	844:878	Furthermore, CTZA NPs significantly enhanced the UV adsorption, antibacterial, and antioxidant properties of the films, while reduced their water vapor permeability.					
37054511	5	27	theme	films	874:878	arg1	antibacterial					825:837	antibacterial	825:837	antibacterial	825:837	Furthermore, CTZA NPs significantly enhanced the UV adsorption, antibacterial, and antioxidant properties of the films, while reduced their water vapor permeability.					
37054511	5	27	theme	films	874:878	arg1	adsorption					813:822	the UV adsorption	806:822	the UV adsorption	806:822	Furthermore, CTZA NPs significantly enhanced the UV adsorption, antibacterial, and antioxidant properties of the films, while reduced their water vapor permeability.					
37054511	0	28	theme	robust	15:20	arg1	films					86:90	robust, water-resistant, antibacterial, and antioxidant chitosan-based films	15:90	robust, water-resistant, antibacterial, and antioxidant chitosan-based films	15:90	Preparation of robust, water-resistant, antibacterial, and antioxidant chitosan-based films by incorporation of cinnamaldehyde-tannin acid-zinc acetate nanoparticles.					
37054511	1	29	theme	food	309:312	arg1	industry					327:334	food preservation industry	309:334	food preservation industry	309:334	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	0	30	theme	acid-zinc	134:142	arg1	acetate					144:150	cinnamaldehyde-tannin acid-zinc acetate	112:150	cinnamaldehyde-tannin acid-zinc acetate	112:150	Preparation of robust, water-resistant, antibacterial, and antioxidant chitosan-based films by incorporation of cinnamaldehyde-tannin acid-zinc acetate nanoparticles.					
37054511	7	31	theme	food	1177:1180	arg1	application					1192:1202	food packaging application	1177:1202	food packaging application	1177:1202	The films with great antibacterial and antioxidant activities can be applied for food packaging application.					
37054511	1	32	theme	preservation	314:325	arg1	industry					327:334	food preservation industry	309:334	food preservation industry	309:334	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	4	33	theme	films	684:688	arg1	sensitivity					666:676	the water sensitivity	656:676	the water sensitivity	656:676	The addition of CTZA NPs reduced the water sensitivity of CS films, which could undergo appreciable stretching in water without breaking.					
37054511	2	34	theme	edible	417:422	arg1	extracts					440:447	edible medicinal plant extracts	417:447	edible medicinal plant extracts	417:447	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts were successfully incorporated into CS films to solve these issues.					
37054511	1	35	theme	low	218:220	arg1	property					208:215	poor mechanical property	192:215	poor mechanical property	192:215	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	1	35	theme	low	218:220	arg1	water-resistance					222:237	low water-resistance	218:237	low water-resistance	218:237	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	0	36	theme	water-resistant	23:37	arg1	films					86:90	robust, water-resistant, antibacterial, and antioxidant chitosan-based films	15:90	robust, water-resistant, antibacterial, and antioxidant chitosan-based films	15:90	Preparation of robust, water-resistant, antibacterial, and antioxidant chitosan-based films by incorporation of cinnamaldehyde-tannin acid-zinc acetate nanoparticles.					
37054511	5	37	theme	vapor	907:911	arg1	permeability					913:924	their water vapor permeability	895:924	their water vapor permeability	895:924	Furthermore, CTZA NPs significantly enhanced the UV adsorption, antibacterial, and antioxidant properties of the films, while reduced their water vapor permeability.					
37054511	1	38	contain	have	187:190	arg2	water-resistance					222:237	low water-resistance	218:237	low water-resistance	218:237	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	1	38	contain	have	187:190	arg1	films					181:185	Chitosan (CS) films	167:185	Chitosan (CS) films	167:185	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	1	38	contain	have	187:190	arg2	activity					265:272	limited antimicrobial activity	243:272	limited antimicrobial activity	243:272	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	1	38	contain	have	187:190	arg2	property					208:215	poor mechanical property	192:215	poor mechanical property	192:215	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	4	39	theme	water	660:664	arg1	sensitivity					666:676	the water sensitivity	656:676	the water sensitivity	656:676	The addition of CTZA NPs reduced the water sensitivity of CS films, which could undergo appreciable stretching in water without breaking.					
37054511	4	40	theme	CS	681:682	arg1	films					684:688	CS films	681:688	CS films	681:688	The addition of CTZA NPs reduced the water sensitivity of CS films, which could undergo appreciable stretching in water without breaking.					
37054511	7	41	with	films	1100:1104	arg1	activities					1147:1156	great antibacterial and antioxidant activities	1111:1156	great antibacterial and antioxidant activities	1111:1156	The films with great antibacterial and antioxidant activities can be applied for food packaging application.					
37054511	2	42	theme	CTZA	392:395	arg1	nanoparticles					377:389	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles	337:389	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts	337:447	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts were successfully incorporated into CS films to solve these issues.					
37054511	2	42	theme	CTZA	392:395	arg1	NPs					397:399	CTZA NPs	392:399	CTZA NPs	392:399	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts were successfully incorporated into CS films to solve these issues.					
37054511	0	43	theme	antibacterial	40:52	arg1	films					86:90	robust, water-resistant, antibacterial, and antioxidant chitosan-based films	15:90	robust, water-resistant, antibacterial, and antioxidant chitosan-based films	15:90	Preparation of robust, water-resistant, antibacterial, and antioxidant chitosan-based films by incorporation of cinnamaldehyde-tannin acid-zinc acetate nanoparticles.					
37054511	3	44	theme	water	542:546	arg1	angle					556:560	water contact angle	542:560	water contact angle	542:560	The tensile strength and water contact angle of the composite films increased about 5.25-fold and 17.55°.					
37054511	3	45	theme	tensile	521:527	arg1	strength					529:536	tensile strength	521:536	tensile strength	521:536	The tensile strength and water contact angle of the composite films increased about 5.25-fold and 17.55°.					
37054511	2	46	theme	acetate	369:375	arg1	nanoparticles					377:389	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles	337:389	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts	337:447	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts were successfully incorporated into CS films to solve these issues.					
37054511	2	46	theme	acetate	369:375	arg1	NPs					397:399	CTZA NPs	392:399	CTZA NPs	392:399	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts were successfully incorporated into CS films to solve these issues.					
37054511	1	47	theme	limited	243:249	arg1	activity					265:272	limited antimicrobial activity	243:272	limited antimicrobial activity	243:272	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	1	47	theme	limited	243:249	arg1	property					208:215	poor mechanical property	192:215	poor mechanical property	192:215	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	0	48	theme	antioxidant	59:69	arg1	films					86:90	robust, water-resistant, antibacterial, and antioxidant chitosan-based films	15:90	robust, water-resistant, antibacterial, and antioxidant chitosan-based films	15:90	Preparation of robust, water-resistant, antibacterial, and antioxidant chitosan-based films by incorporation of cinnamaldehyde-tannin acid-zinc acetate nanoparticles.					
37054511	6	49	theme	carbon	1061:1066	arg1	powder					1068:1073	carbon powder	1061:1073	carbon powder	1061:1073	Moreover, it was possible to print inks onto the films because the presence of the hydrophobic CTZA NPs facilitated the deposition of carbon powder onto their surfaces.					
37054511	1	50	theme	Chitosan	167:174	arg1	films					181:185	Chitosan (CS) films	167:185	Chitosan (CS) films	167:185	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	5	51	theme	water	901:905	arg1	permeability					913:924	their water vapor permeability	895:924	their water vapor permeability	895:924	Furthermore, CTZA NPs significantly enhanced the UV adsorption, antibacterial, and antioxidant properties of the films, while reduced their water vapor permeability.					
37054511	1	52	theme	antimicrobial	251:263	arg1	activity					265:272	limited antimicrobial activity	243:272	limited antimicrobial activity	243:272	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	1	52	theme	antimicrobial	251:263	arg1	property					208:215	poor mechanical property	192:215	poor mechanical property	192:215	Chitosan (CS) films have poor mechanical property, low water-resistance and limited antimicrobial activity, which hinder their application in food preservation industry.					
37054511	6	53	theme	hydrophobic	1010:1020	arg1	NPs					1027:1029	the hydrophobic CTZA NPs	1006:1029	the hydrophobic CTZA NPs	1006:1029	Moreover, it was possible to print inks onto the films because the presence of the hydrophobic CTZA NPs facilitated the deposition of carbon powder onto their surfaces.					
37054511	6	54	theme	print	956:960	arg1	inks					962:965	print inks	956:965	print inks onto the films	956:980	Moreover, it was possible to print inks onto the films because the presence of the hydrophobic CTZA NPs facilitated the deposition of carbon powder onto their surfaces.					
37054511	2	55	theme	CS	485:486	arg1	films					488:492	CS films	485:492	CS films	485:492	Cinnamaldehyde-tannic acid-zinc acetate nanoparticles (CTZA NPs) assembled from edible medicinal plant extracts were successfully incorporated into CS films to solve these issues.					
37054511	4	56	theme	appreciable	711:721	arg1	stretching					723:732	appreciable stretching	711:732	appreciable stretching	711:732	The addition of CTZA NPs reduced the water sensitivity of CS films, which could undergo appreciable stretching in water without breaking.					
35351551	0	0	theme	biodegradable	72:84	arg1	films					121:125	biodegradable and edible gelatin and corn starch films	72:125	biodegradable and edible gelatin and corn starch films	72:125	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.					
35351551	7	1	theme	%	1085:1085	arg1	reduction					1087:1095	a 15% reduction	1081:1095	a 15% reduction in water vapor permeability	1081:1123	Furthermore, at this concentration, a 15% reduction in water vapor permeability was observed.					
35351551	9	2	with	films	1256:1260	arg1	extract					1279:1285	corn stigma extract	1267:1285	corn stigma extract	1267:1285	It can be inferred that the films with corn stigma extract have good antioxidant properties, indicating that they can be used as an ingredient for food packaging.					
35351551	7	3	theme	vapor	1106:1110	arg1	permeability					1112:1123	water vapor permeability	1100:1123	water vapor permeability	1100:1123	Furthermore, at this concentration, a 15% reduction in water vapor permeability was observed.					
35351551	9	4	theme	stigma	1272:1277	arg1	extract					1279:1285	corn stigma extract	1267:1285	corn stigma extract	1267:1285	It can be inferred that the films with corn stigma extract have good antioxidant properties, indicating that they can be used as an ingredient for food packaging.					
35351551	2	5	dep	%	422:422	arg1	w/v					433:435	w/v	433:435	15% and 25%; w/v	420:435	In this study, bioactive films were developed based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v).					
35351551	1	6	theme	bio-based	147:155	arg1	packaging					162:170	bio-based food packaging	147:170	bio-based food packaging with antioxidant properties	147:198	The development of bio-based food packaging with antioxidant properties is an important research topic and has gained prominence these days.					
35351551	0	7	theme	edible	90:95	arg1	films					121:125	biodegradable and edible gelatin and corn starch films	72:125	biodegradable and edible gelatin and corn starch films	72:125	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.					
35351551	0	8	from	Effect	0:5	arg1	properties					58:67	physical and antioxidant properties	33:67	physical and antioxidant properties of biodegradable and edible gelatin and corn starch films	33:125	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.					
35351551	5	9	theme	mechanical	873:882	arg1	properties					884:893	the mechanical properties	869:893	the mechanical properties (elongation at break, Young's modulus)	869:932	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	6	10	theme	a	1020:1020	arg1	character					1034:1042	the film a hydrophobic character	1011:1042	the film a hydrophobic character	1011:1042	The addition of 25% corn stigma extract increased the contact angle, giving the film a hydrophobic character.					
35351551	7	11	theme	15	1083:1084	arg1	%					1085:1085	%	1085:1085	%	1085:1085	Furthermore, at this concentration, a 15% reduction in water vapor permeability was observed.					
35351551	3	12	theme	cell	484:487	arg1	viability					489:497	cell viability	484:497	cell viability	484:497	In preliminary tests, the extract maintained cell viability above 90% indicating that it is safe for application as an active ingredient.					
35351551	9	13	theme	good	1292:1295	arg1	properties					1309:1318	good antioxidant properties	1292:1318	good antioxidant properties	1292:1318	It can be inferred that the films with corn stigma extract have good antioxidant properties, indicating that they can be used as an ingredient for food packaging.					
35351551	5	14	theme	starch	827:832	arg1	compounds					834:842	corn starch compounds	822:842	corn starch compounds	822:842	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	2	15	dep	concentrations	404:417	arg1	%					422:422	15%	420:422	15%	420:422	In this study, bioactive films were developed based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v).					
35351551	2	15	dep	concentrations	404:417	arg1	%					430:430	25%	428:430	25%	428:430	In this study, bioactive films were developed based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v).					
35351551	6	16	theme	extract	967:973	arg1	addition					939:946	The addition	935:946	The addition of 25% corn stigma extract	935:973	The addition of 25% corn stigma extract increased the contact angle, giving the film a hydrophobic character.					
35351551	4	17	theme	extract	594:600	arg1	Insertion					577:585	Insertion	577:585	Insertion of the extract	577:600	Insertion of the extract did not influence the thickness of the films but caused a slight change in optical properties.					
35351551	1	18	theme	food	157:160	arg1	packaging					162:170	bio-based food packaging	147:170	bio-based food packaging with antioxidant properties	147:198	The development of bio-based food packaging with antioxidant properties is an important research topic and has gained prominence these days.					
35351551	0	19	theme	gelatin	97:103	arg1	films					121:125	biodegradable and edible gelatin and corn starch films	72:125	biodegradable and edible gelatin and corn starch films	72:125	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.					
35351551	5	20	theme	corn	822:825	arg1	compounds					834:842	corn starch compounds	822:842	corn starch compounds	822:842	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	8	21	theme	complete	1167:1174	arg1	biodegradability					1176:1191	complete biodegradability	1167:1191	complete biodegradability	1167:1191	The elaborated films showed complete biodegradability before the tenth day of the study.					
35351551	4	22	theme	optical	677:683	arg1	properties					685:694	optical properties	677:694	optical properties	677:694	Insertion of the extract did not influence the thickness of the films but caused a slight change in optical properties.					
35351551	1	23	theme	packaging	162:170	arg1	development					132:142	The development	128:142	The development of bio-based food packaging with antioxidant properties	128:198	The development of bio-based food packaging with antioxidant properties is an important research topic and has gained prominence these days.					
35351551	1	23	theme	packaging	162:170	arg1	topic					225:229	an important research topic	203:229	an important research topic	203:229	The development of bio-based food packaging with antioxidant properties is an important research topic and has gained prominence these days.					
35351551	9	24	contain	have	1287:1290	arg1	films					1256:1260	the films	1252:1260	the films with corn stigma extract	1252:1285	It can be inferred that the films with corn stigma extract have good antioxidant properties, indicating that they can be used as an ingredient for food packaging.					
35351551	9	24	contain	have	1287:1290	arg2	properties					1309:1318	good antioxidant properties	1292:1318	good antioxidant properties	1292:1318	It can be inferred that the films with corn stigma extract have good antioxidant properties, indicating that they can be used as an ingredient for food packaging.					
35351551	5	25	from	break	910:914	arg1	modulus					925:931	Young's modulus	917:931	Young's modulus	917:931	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	5	25	from	break	910:914	arg1	elongation					896:905	elongation	896:905	elongation at break	896:914	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	2	26	theme	corn	365:368	arg1	CSE					386:388	CSE	386:388	CSE	386:388	In this study, bioactive films were developed based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v).					
35351551	2	26	theme	corn	365:368	arg1	extract					377:383	corn stigma extract	365:383	corn stigma extract (CSE)	365:389	In this study, bioactive films were developed based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v).					
35351551	5	27	with	compounds	834:842	arg1	gelatin					810:816	gelatin	810:816	gelatin	810:816	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	1	28	theme	antioxidant	177:187	arg1	properties					189:198	antioxidant properties	177:198	antioxidant properties	177:198	The development of bio-based food packaging with antioxidant properties is an important research topic and has gained prominence these days.					
35351551	0	29	theme	stigma	15:20	arg1	extract					22:28	corn stigma extract	10:28	corn stigma extract	10:28	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.					
35351551	6	30	theme	stigma	960:965	arg1	extract					967:973	25% corn stigma extract	951:973	25% corn stigma extract	951:973	The addition of 25% corn stigma extract increased the contact angle, giving the film a hydrophobic character.					
35351551	0	31	theme	starch	114:119	arg1	films					121:125	biodegradable and edible gelatin and corn starch films	72:125	biodegradable and edible gelatin and corn starch films	72:125	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.					
35351551	9	32	theme	antioxidant	1297:1307	arg1	properties					1309:1318	good antioxidant properties	1292:1318	good antioxidant properties	1292:1318	It can be inferred that the films with corn stigma extract have good antioxidant properties, indicating that they can be used as an ingredient for food packaging.					
35351551	2	33	theme	bioactive	284:292	arg1	films					294:298	bioactive films	284:298	bioactive films	284:298	In this study, bioactive films were developed based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v).					
35351551	6	34	theme	25	951:952	arg1	%					953:953	%	953:953	%	953:953	The addition of 25% corn stigma extract increased the contact angle, giving the film a hydrophobic character.					
35351551	0	35	theme	corn	10:13	arg1	extract					22:28	corn stigma extract	10:28	corn stigma extract	10:28	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.					
35351551	0	36	theme	corn	109:112	arg1	films					121:125	biodegradable and edible gelatin and corn starch films	72:125	biodegradable and edible gelatin and corn starch films	72:125	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.					
35351551	3	37	theme	preliminary	442:452	arg1	tests					454:458	preliminary tests	442:458	preliminary tests	442:458	In preliminary tests, the extract maintained cell viability above 90% indicating that it is safe for application as an active ingredient.					
35351551	6	38	theme	corn	955:958	arg1	extract					967:973	25% corn stigma extract	951:973	25% corn stigma extract	951:973	The addition of 25% corn stigma extract increased the contact angle, giving the film a hydrophobic character.					
35351551	6	39	theme	%	953:953	arg1	extract					967:973	25% corn stigma extract	951:973	25% corn stigma extract	951:973	The addition of 25% corn stigma extract increased the contact angle, giving the film a hydrophobic character.					
35351551	0	40	theme	extract	22:28	arg1	Effect					0:5	Effect	0:5	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.	0:126	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.					
35351551	0	41	theme	films	121:125	arg1	properties					58:67	physical and antioxidant properties	33:67	physical and antioxidant properties of biodegradable and edible gelatin and corn starch films	33:125	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.					
35351551	1	42	theme	important	206:214	arg1	development					132:142	The development	128:142	The development of bio-based food packaging with antioxidant properties	128:198	The development of bio-based food packaging with antioxidant properties is an important research topic and has gained prominence these days.					
35351551	1	42	theme	important	206:214	arg1	topic					225:229	an important research topic	203:229	an important research topic	203:229	The development of bio-based food packaging with antioxidant properties is an important research topic and has gained prominence these days.					
35351551	5	43	theme	electron	706:713	arg1	SEM					727:729	SEM	727:729	SEM	727:729	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	5	43	theme	electron	706:713	arg1	microscopy					715:724	Scanning electron microscopy	697:724	Scanning electron microscopy (SEM) analysis	697:739	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	5	44	theme	bioactive	785:793	arg1	compounds					795:803	the extract's bioactive compounds	771:803	the extract's bioactive compounds with gelatin	771:816	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	5	45	theme	microscopy	715:724	arg1	analysis					732:739	Scanning electron microscopy (SEM) analysis	697:739	Scanning electron microscopy (SEM) analysis	697:739	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	3	46	theme	active	558:563	arg1	ingredient					565:574	an active ingredient	555:574	an active ingredient	555:574	In preliminary tests, the extract maintained cell viability above 90% indicating that it is safe for application as an active ingredient.					
35351551	2	47	theme	starch	334:339	arg1	GCS					342:344	based gelatin-corn starch (GCS)	315:345	based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v)	315:436	In this study, bioactive films were developed based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v).					
35351551	0	48	theme	physical	33:40	arg1	properties					58:67	physical and antioxidant properties	33:67	physical and antioxidant properties of biodegradable and edible gelatin and corn starch films	33:125	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.					
35351551	5	49	with	compounds	795:803	arg1	gelatin					810:816	gelatin	810:816	gelatin	810:816	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	6	50	theme	hydrophobic	1022:1032	arg1	character					1034:1042	the film a hydrophobic character	1011:1042	the film a hydrophobic character	1011:1042	The addition of 25% corn stigma extract increased the contact angle, giving the film a hydrophobic character.					
35351551	2	51	theme	gelatin-corn	321:332	arg1	GCS					342:344	based gelatin-corn starch (GCS)	315:345	based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v)	315:436	In this study, bioactive films were developed based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v).					
35351551	5	52	theme	Young	917:921	arg1	modulus					925:931	Young's modulus	917:931	Young's modulus	917:931	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	5	52	theme	Young	917:921	arg1	elongation					896:905	elongation	896:905	elongation at break	896:914	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	7	53	from	reduction	1087:1095	arg1	permeability					1112:1123	water vapor permeability	1100:1123	water vapor permeability	1100:1123	Furthermore, at this concentration, a 15% reduction in water vapor permeability was observed.					
35351551	5	54	theme	Scanning	697:704	arg1	SEM					727:729	SEM	727:729	SEM	727:729	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	5	54	theme	Scanning	697:704	arg1	microscopy					715:724	Scanning electron microscopy	697:724	Scanning electron microscopy (SEM) analysis	697:739	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	9	55	theme	food	1375:1378	arg1	packaging					1380:1388	food packaging	1375:1388	food packaging	1375:1388	It can be inferred that the films with corn stigma extract have good antioxidant properties, indicating that they can be used as an ingredient for food packaging.					
35351551	2	56	theme	based	315:319	arg1	GCS					342:344	based gelatin-corn starch (GCS)	315:345	based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v)	315:436	In this study, bioactive films were developed based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v).					
35351551	4	57	theme	slight	660:665	arg1	change					667:672	a slight change	658:672	a slight change	658:672	Insertion of the extract did not influence the thickness of the films but caused a slight change in optical properties.					
35351551	1	58	theme	research	216:223	arg1	development					132:142	The development	128:142	The development of bio-based food packaging with antioxidant properties	128:198	The development of bio-based food packaging with antioxidant properties is an important research topic and has gained prominence these days.					
35351551	1	58	theme	research	216:223	arg1	topic					225:229	an important research topic	203:229	an important research topic	203:229	The development of bio-based food packaging with antioxidant properties is an important research topic and has gained prominence these days.					
35351551	1	59	with	packaging	162:170	arg1	properties					189:198	antioxidant properties	177:198	antioxidant properties	177:198	The development of bio-based food packaging with antioxidant properties is an important research topic and has gained prominence these days.					
35351551	8	60	theme	study	1221:1225	arg1	day					1210:1212	the tenth day	1200:1212	the tenth day of the study	1200:1225	The elaborated films showed complete biodegradability before the tenth day of the study.					
35351551	5	61	dep	properties	884:893	arg1	modulus					925:931	Young's modulus	917:931	Young's modulus	917:931	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	5	61	dep	properties	884:893	arg1	elongation					896:905	elongation	896:905	elongation at break	896:914	Scanning electron microscopy (SEM) analysis revealed interactions between the extract's bioactive compounds with gelatin and corn starch compounds, which may have improved the mechanical properties (elongation at break, Young's modulus).					
35351551	6	62	theme	film	1015:1018	arg1	character					1034:1042	the film a hydrophobic character	1011:1042	the film a hydrophobic character	1011:1042	The addition of 25% corn stigma extract increased the contact angle, giving the film a hydrophobic character.					
35351551	4	63	theme	films	641:645	arg1	thickness					624:632	the thickness	620:632	the thickness of the films	620:645	Insertion of the extract did not influence the thickness of the films but caused a slight change in optical properties.					
35351551	7	64	theme	water	1100:1104	arg1	permeability					1112:1123	water vapor permeability	1100:1123	water vapor permeability	1100:1123	Furthermore, at this concentration, a 15% reduction in water vapor permeability was observed.					
35351551	8	65	theme	elaborated	1143:1152	arg1	films					1154:1158	The elaborated films	1139:1158	The elaborated films	1139:1158	The elaborated films showed complete biodegradability before the tenth day of the study.					
35351551	8	66	theme	tenth	1204:1208	arg1	day					1210:1212	the tenth day	1200:1212	the tenth day of the study	1200:1225	The elaborated films showed complete biodegradability before the tenth day of the study.					
35351551	2	67	theme	stigma	370:375	arg1	CSE					386:388	CSE	386:388	CSE	386:388	In this study, bioactive films were developed based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v).					
35351551	2	67	theme	stigma	370:375	arg1	extract					377:383	corn stigma extract	365:383	corn stigma extract (CSE)	365:389	In this study, bioactive films were developed based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v).					
35351551	0	68	theme	antioxidant	46:56	arg1	properties					58:67	physical and antioxidant properties	33:67	physical and antioxidant properties of biodegradable and edible gelatin and corn starch films	33:125	Effect of corn stigma extract on physical and antioxidant properties of biodegradable and edible gelatin and corn starch films.					
35351551	7	69	located	observed	1129:1136	arg1	concentration					1066:1078	this concentration	1061:1078	this concentration	1061:1078	Furthermore, at this concentration, a 15% reduction in water vapor permeability was observed.					
35351551	7	69	located	observed	1129:1136	arg2	reduction					1087:1095	a 15% reduction	1081:1095	a 15% reduction in water vapor permeability	1081:1123	Furthermore, at this concentration, a 15% reduction in water vapor permeability was observed.					
35351551	2	70	theme	different	394:402	arg1	concentrations					404:417	different concentrations	394:417	different concentrations (15% and 25%; w/v)	394:436	In this study, bioactive films were developed based gelatin-corn starch (GCS) incorporated with corn stigma extract (CSE) at different concentrations (15% and 25%; w/v).					
35351551	9	71	used	used	1349:1352	arg2	they					1337:1340	they	1337:1340	they	1337:1340	It can be inferred that the films with corn stigma extract have good antioxidant properties, indicating that they can be used as an ingredient for food packaging.					
35351551	9	71	used	used	1349:1352	arg2	ingredient					1360:1369	an ingredient	1357:1369	an ingredient for food packaging	1357:1388	It can be inferred that the films with corn stigma extract have good antioxidant properties, indicating that they can be used as an ingredient for food packaging.					
35351551	6	72	theme	contact	989:995	arg1	angle					997:1001	the contact angle	985:1001	the contact angle	985:1001	The addition of 25% corn stigma extract increased the contact angle, giving the film a hydrophobic character.					
35351551	9	73	theme	corn	1267:1270	arg1	extract					1279:1285	corn stigma extract	1267:1285	corn stigma extract	1267:1285	It can be inferred that the films with corn stigma extract have good antioxidant properties, indicating that they can be used as an ingredient for food packaging.					
36907757	4	0	theme	treatment	777:785	arg1	group					787:791	PBLC treatment group	772:791	PBLC treatment group	772:791	A total of 29 BS cows and 41 HF cows were divided each into a control (CON) and PBLC treatment group.					
36907757	3	1	theme	milk	650:653	arg1	performance					655:665	milk performance	650:665	milk performance	650:665	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	7	2	theme	breed	1024:1028	arg1	interaction					1042:1052	a significant breed × treatment interaction	1010:1052	a significant breed × treatment interaction for iCa	1010:1060	Feeding PBLC induced a significant breed × treatment interaction for iCa, supporting that PBLC increased iCa exclusively in HF cows; the increase was 0.03 mM over the whole period and 0.05 mM from d 1 to 3 after calving.					
36907757	3	3	from	minerals	503:510	arg1	Swiss					521:525	Swiss	521:525	Swiss	521:525	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	16	4	theme	positive	2478:2485	arg1	effects					2487:2493	additional positive effects	2467:2493	additional positive effects	2467:2493	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	3	5	theme	lactation	681:689	arg1	d					673:673	d 80	673:676	d 80 of lactation	673:689	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	13	6	theme	lactose	1962:1968	arg1	yield					1970:1974	milk lactose yield	1957:1974	milk lactose yield	1957:1974	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	11	7	theme	condition	1626:1634	arg1	score					1636:1640	Body condition score	1621:1640	Body condition score	1621:1640	Body condition score showed no effect of treatment, except for a lower body condition score in BS-PBLC compared with BS-CON at d 14.					
36907757	16	8	theme	milk	2498:2501	arg1	performance					2503:2513	milk performance	2498:2513	milk performance in both breeds	2498:2528	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	10	9	theme	PBLC	1508:1511	arg1	feeding					1513:1519	PBLC feeding	1508:1519	PBLC feeding	1508:1519	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	13	10	theme	test	2012:2015	arg1	day					2017:2019	the first test day	2002:2019	the first test day	2002:2019	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	16	11	theme	calcium	2413:2419	arg1	status					2421:2426	calcium status	2413:2426	calcium status	2413:2426	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	10	12	theme	blood	1400:1404	arg1	minerals					1406:1413	Other tested blood minerals	1387:1413	Other tested blood minerals	1387:1413	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	10	12	theme	blood	1400:1404	arg1	chloride					1432:1439	chloride	1432:1439	chloride	1432:1439	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	10	12	theme	blood	1400:1404	arg1	potassium					1446:1454	potassium	1446:1454	potassium	1446:1454	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	10	12	theme	blood	1400:1404	arg1	sodium					1424:1429	sodium	1424:1429	sodium	1424:1429	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	15	13	theme	weekly	2220:2225	arg1	yield					2232:2236	The weekly milk yield	2216:2236	The weekly milk yield over the first 11 wk of lactation	2216:2270	The weekly milk yield over the first 11 wk of lactation was 29.5 kg/wk higher for PBLC versus CON across breeds.					
36907757	15	13	theme	weekly	2220:2225	arg1	higher					2287:2292	higher	2287:2292	higher	2287:2292	The weekly milk yield over the first 11 wk of lactation was 29.5 kg/wk higher for PBLC versus CON across breeds.					
36907757	1	14	theme	bioactive	189:197	arg1	compounds					205:213	plant bioactive lipid compounds	183:213	plant bioactive lipid compounds (PBLC)	183:220	Previous studies ex vivo suggested that plant bioactive lipid compounds (PBLC) can increase ruminal calcium absorption.					
36907757	1	14	theme	bioactive	189:197	arg1	PBLC					216:219	PBLC	216:219	PBLC	216:219	Previous studies ex vivo suggested that plant bioactive lipid compounds (PBLC) can increase ruminal calcium absorption.					
36907757	2	15	from	performance	375:385	arg1	cows					407:410	postpartum dairy cows	390:410	postpartum dairy cows	390:410	Therefore, we hypothesized that PBLC feeding around calving may potentially counteract hypocalcemia and support performance in postpartum dairy cows.					
36907757	9	16	dep	cows	1354:1357	arg1	HF-CON					1362:1367	2 HF-CON	1360:1367	2 HF-CON	1360:1367	Clinical milk fever was detected only in HF cows (2 HF-CON and one HF-PBLC).					
36907757	9	16	dep	cows	1354:1357	arg1	HF-PBLC					1377:1383	one HF-PBLC	1373:1383	one HF-PBLC	1373:1383	Clinical milk fever was detected only in HF cows (2 HF-CON and one HF-PBLC).					
36907757	9	16	dep	cows	1354:1357	arg1	cows					1354:1357	HF cows	1351:1357	HF cows (2 HF-CON and one HF-PBLC)	1351:1384	Clinical milk fever was detected only in HF cows (2 HF-CON and one HF-PBLC).					
36907757	0	17	theme	bioactive	116:124	arg1	compounds					132:140	plant bioactive lipid compounds	110:140	plant bioactive lipid compounds	110:140	Blood calcium concentration and performance in periparturient and early lactating dairy cows is influenced by plant bioactive lipid compounds.					
36907757	3	18	theme	Holstein	561:568	arg1	cows					584:587	hypocalcemia-susceptible Holstein Friesian (HF) cows	536:587	hypocalcemia-susceptible Holstein Friesian (HF) cows	536:587	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	9	19	located	detected	1334:1341	arg1	HF-CON					1362:1367	2 HF-CON	1360:1367	2 HF-CON	1360:1367	Clinical milk fever was detected only in HF cows (2 HF-CON and one HF-PBLC).					
36907757	9	19	located	detected	1334:1341	arg1	HF-PBLC					1377:1383	one HF-PBLC	1373:1383	one HF-PBLC	1373:1383	Clinical milk fever was detected only in HF cows (2 HF-CON and one HF-PBLC).					
36907757	9	19	located	detected	1334:1341	arg2	fever					1324:1328	Clinical milk fever	1310:1328	Clinical milk fever	1310:1328	Clinical milk fever was detected only in HF cows (2 HF-CON and one HF-PBLC).					
36907757	9	19	located	detected	1334:1341	arg1	cows					1354:1357	HF cows	1351:1357	HF cows (2 HF-CON and one HF-PBLC)	1351:1384	Clinical milk fever was detected only in HF cows (2 HF-CON and one HF-PBLC).					
36907757	2	20	theme	postpartum	390:399	arg1	cows					407:410	postpartum dairy cows	390:410	postpartum dairy cows	390:410	Therefore, we hypothesized that PBLC feeding around calving may potentially counteract hypocalcemia and support performance in postpartum dairy cows.					
36907757	12	21	theme	milk	1809:1812	arg1	yield					1822:1826	milk protein yield	1809:1826	milk protein yield	1809:1826	Dietary PBLC increased milk yield, milk fat yield, and milk protein yield at 2 consecutive dairy herd improvement test days.					
36907757	11	22	theme	lower	1686:1690	arg1	score					1707:1711	a lower body condition score	1684:1711	a lower body condition score in BS-PBLC compared with BS-CON at d 14	1684:1751	Body condition score showed no effect of treatment, except for a lower body condition score in BS-PBLC compared with BS-CON at d 14.					
36907757	3	23	dep	d	612:612	arg1	to					617:618	to	617:618	to	617:618	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	16	24	theme	study	2446:2450	arg1	period					2452:2457	the study period	2442:2457	the study period	2442:2457	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	10	25	theme	2-way	1547:1551	arg1	interactions					1553:1564	their 2-way interactions	1541:1564	their 2-way interactions	1541:1564	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	8	26	theme	HF-PBLC	1296:1302	arg1	cows					1304:1307	2 BS-PBLC and 4 HF-PBLC cows	1280:1307	cows	1304:1307	Subclinical hypocalcemia was seen in one BS-CON and 8 HF-CON cows and 2 BS-PBLC and 4 HF-PBLC cows.					
36907757	12	27	theme	fat	1794:1796	arg1	yield					1798:1802	milk fat yield	1789:1802	milk fat yield	1789:1802	Dietary PBLC increased milk yield, milk fat yield, and milk protein yield at 2 consecutive dairy herd improvement test days.					
36907757	10	28	theme	sodium	1585:1590	arg1	levels					1592:1597	higher sodium levels	1578:1597	higher sodium levels in PBLC cows on d 21	1578:1618	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	13	29	theme	milk	1942:1945	arg1	yield					1947:1951	energy-corrected milk yield	1925:1951	energy-corrected milk yield	1925:1951	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	5	30	theme	menthol-rich	835:846	arg1	PBLC					848:851	1.7 g/d menthol-rich PBLC	827:851	1.7 g/d menthol-rich PBLC from 8 d	827:860	The latter was supplemented with 1.7 g/d menthol-rich PBLC from 8 d before expected calving to 80 d postpartum.					
36907757	16	31	theme	additional	2467:2476	arg1	effects					2487:2493	additional positive effects	2467:2493	additional positive effects	2467:2493	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	0	32	theme	early	66:70	arg1	cows					88:91	periparturient and early lactating dairy cows	47:91	periparturient and early lactating dairy cows	47:91	Blood calcium concentration and performance in periparturient and early lactating dairy cows is influenced by plant bioactive lipid compounds.					
36907757	11	33	theme	condition	1697:1705	arg1	score					1707:1711	a lower body condition score	1684:1711	a lower body condition score in BS-PBLC compared with BS-CON at d 14	1684:1751	Body condition score showed no effect of treatment, except for a lower body condition score in BS-PBLC compared with BS-CON at d 14.					
36907757	6	34	theme	Milk	906:909	arg1	yield					911:915	Milk yield	906:915	Milk yield	906:915	Milk yield and composition, body condition score and blood minerals were measured.					
36907757	0	35	from	concentration	14:26	arg1	cows					88:91	periparturient and early lactating dairy cows	47:91	periparturient and early lactating dairy cows	47:91	Blood calcium concentration and performance in periparturient and early lactating dairy cows is influenced by plant bioactive lipid compounds.					
36907757	12	36	theme	milk	1777:1780	arg1	yield					1782:1786	milk yield	1777:1786	milk yield	1777:1786	Dietary PBLC increased milk yield, milk fat yield, and milk protein yield at 2 consecutive dairy herd improvement test days.					
36907757	0	37	theme	dairy	82:86	arg1	cows					88:91	periparturient and early lactating dairy cows	47:91	periparturient and early lactating dairy cows	47:91	Blood calcium concentration and performance in periparturient and early lactating dairy cows is influenced by plant bioactive lipid compounds.					
36907757	13	38	theme	milk	1957:1960	arg1	yield					1970:1974	milk lactose yield	1957:1974	milk lactose yield	1957:1974	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	4	39	theme	cows	709:712	arg1	total					694:698	A total	692:698	A total of 29 BS cows and 41 HF cows	692:727	A total of 29 BS cows and 41 HF cows were divided each into a control (CON) and PBLC treatment group.					
36907757	15	40	theme	lactation	2262:2270	arg1	wk					2256:2257	the first 11 wk	2243:2257	the first 11 wk of lactation	2243:2270	The weekly milk yield over the first 11 wk of lactation was 29.5 kg/wk higher for PBLC versus CON across breeds.					
36907757	16	41	theme	small	2377:2381	arg1	improvement					2398:2408	a small but measurable improvement	2375:2408	a small but measurable improvement of calcium status in HF cows	2375:2437	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	5	42	theme	expected	869:876	arg1	calving					878:884	expected calving	869:884	expected calving to 80 d postpartum	869:903	The latter was supplemented with 1.7 g/d menthol-rich PBLC from 8 d before expected calving to 80 d postpartum.					
36907757	14	43	theme	cell	2172:2175	arg1	count					2177:2181	somatic cell count	2164:2181	somatic cell count	2164:2181	The concentrations of fat, lactose, and urea, as well as somatic cell count, were not affected by treatment.					
36907757	0	44	from	performance	32:42	arg1	cows					88:91	periparturient and early lactating dairy cows	47:91	periparturient and early lactating dairy cows	47:91	Blood calcium concentration and performance in periparturient and early lactating dairy cows is influenced by plant bioactive lipid compounds.					
36907757	1	45	dep	ex	160:161	arg1	vivo					163:166	vivo	163:166	vivo	163:166	Previous studies ex vivo suggested that plant bioactive lipid compounds (PBLC) can increase ruminal calcium absorption.					
36907757	3	46	from	d	612:612	arg1	period					600:605	the period	596:605	the period from d -2 to 28 relative to calving	596:641	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	2	47	theme	dairy	401:405	arg1	cows					407:410	postpartum dairy cows	390:410	postpartum dairy cows	390:410	Therefore, we hypothesized that PBLC feeding around calving may potentially counteract hypocalcemia and support performance in postpartum dairy cows.					
36907757	0	48	theme	Blood	0:4	arg1	concentration					14:26	Blood calcium concentration	0:26	Blood calcium concentration	0:26	Blood calcium concentration and performance in periparturient and early lactating dairy cows is influenced by plant bioactive lipid compounds.					
36907757	13	49	theme	day	1907:1909	arg1	interactions					1911:1922	treatment × day interactions	1895:1922	treatment × day interactions	1895:1922	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	10	50	theme	higher	1578:1583	arg1	levels					1592:1597	higher sodium levels	1578:1597	higher sodium levels in PBLC cows on d 21	1578:1618	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	11	51	theme	treatment	1662:1670	arg1	effect					1652:1657	no effect	1649:1657	no effect of treatment	1649:1670	Body condition score showed no effect of treatment, except for a lower body condition score in BS-PBLC compared with BS-CON at d 14.					
36907757	14	52	theme	somatic	2164:2170	arg1	count					2177:2181	somatic cell count	2164:2181	somatic cell count	2164:2181	The concentrations of fat, lactose, and urea, as well as somatic cell count, were not affected by treatment.					
36907757	13	53	theme	test	2085:2088	arg1	d					2090:2090	test d 2	2085:2092	test d 2	2085:2092	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	10	54	from	levels	1592:1597	arg1	cows					1607:1610	PBLC cows	1602:1610	PBLC cows on d 21	1602:1618	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	10	54	from	levels	1592:1597	arg1	d					1615:1615	d 21	1615:1618	d 21	1615:1618	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	6	55	theme	condition	939:947	arg1	score					949:953	body condition score	934:953	body condition score	934:953	Milk yield and composition, body condition score and blood minerals were measured.					
36907757	16	56	contain	had	2463:2465	arg1	PBLC					2362:2365	the applied PBLC	2350:2365	the applied PBLC	2350:2365	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	16	56	contain	had	2463:2465	arg2	effects					2487:2493	additional positive effects	2467:2493	additional positive effects	2467:2493	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	3	57	theme	relative	623:630	arg1	d					612:612	d -2 to 28	612:621	d -2 to 28 relative to calving	612:641	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	7	58	dep	d	1186:1186	arg1	to					1190:1191	to	1190:1191	to	1190:1191	Feeding PBLC induced a significant breed × treatment interaction for iCa, supporting that PBLC increased iCa exclusively in HF cows; the increase was 0.03 mM over the whole period and 0.05 mM from d 1 to 3 after calving.					
36907757	0	59	theme	periparturient	47:60	arg1	cows					88:91	periparturient and early lactating dairy cows	47:91	periparturient and early lactating dairy cows	47:91	Blood calcium concentration and performance in periparturient and early lactating dairy cows is influenced by plant bioactive lipid compounds.					
36907757	13	60	theme	treatment	1895:1903	arg1	interactions					1911:1922	treatment × day interactions	1895:1922	treatment × day interactions	1895:1922	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	13	61	theme	test	2073:2076	arg1	d					2078:2078	test d 1	2073:2080	test d 1	2073:2080	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	7	62	theme	whole	1156:1160	arg1	period					1162:1167	the whole period	1152:1167	the whole period	1152:1167	Feeding PBLC induced a significant breed × treatment interaction for iCa, supporting that PBLC increased iCa exclusively in HF cows; the increase was 0.03 mM over the whole period and 0.05 mM from d 1 to 3 after calving.					
36907757	8	63	theme	HF-CON	1264:1269	arg1	cows					1271:1274	one BS-CON and 8 HF-CON cows	1247:1274	one BS-CON and 8 HF-CON cows	1247:1274	Subclinical hypocalcemia was seen in one BS-CON and 8 HF-CON cows and 2 BS-PBLC and 4 HF-PBLC cows.					
36907757	10	64	theme	blood	1468:1472	arg1	glucose					1474:1480	blood glucose	1468:1480	blood glucose	1468:1480	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	5	65	from	d	860:860	arg1	PBLC					848:851	1.7 g/d menthol-rich PBLC	827:851	1.7 g/d menthol-rich PBLC from 8 d	827:860	The latter was supplemented with 1.7 g/d menthol-rich PBLC from 8 d before expected calving to 80 d postpartum.					
36907757	5	66	theme	d	892:892	arg1	postpartum					894:903	80 d postpartum	889:903	80 d postpartum	889:903	The latter was supplemented with 1.7 g/d menthol-rich PBLC from 8 d before expected calving to 80 d postpartum.					
36907757	12	67	theme	improvement	1856:1866	arg1	days					1873:1876	2 consecutive dairy herd improvement test days	1831:1876	2 consecutive dairy herd improvement test days	1831:1876	Dietary PBLC increased milk yield, milk fat yield, and milk protein yield at 2 consecutive dairy herd improvement test days.					
36907757	14	68	theme	lactose	2134:2140	arg1	concentrations					2111:2124	The concentrations	2107:2124	The concentrations of fat, lactose, and urea	2107:2150	The concentrations of fat, lactose, and urea, as well as somatic cell count, were not affected by treatment.					
36907757	14	68	theme	lactose	2134:2140	arg1	count					2177:2181	somatic cell count	2164:2181	somatic cell count	2164:2181	The concentrations of fat, lactose, and urea, as well as somatic cell count, were not affected by treatment.					
36907757	13	69	theme	protein	2036:2042	arg1	concentration					2044:2056	milk protein concentration	2031:2056	milk protein concentration	2031:2056	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	7	70	theme	significant	1012:1022	arg1	interaction					1042:1052	a significant breed × treatment interaction	1010:1052	a significant breed × treatment interaction for iCa	1010:1060	Feeding PBLC induced a significant breed × treatment interaction for iCa, supporting that PBLC increased iCa exclusively in HF cows; the increase was 0.03 mM over the whole period and 0.05 mM from d 1 to 3 after calving.					
36907757	2	71	theme	support	367:373	arg1	performance					375:385	support performance	367:385	support performance	367:385	Therefore, we hypothesized that PBLC feeding around calving may potentially counteract hypocalcemia and support performance in postpartum dairy cows.					
36907757	1	72	theme	calcium	243:249	arg1	absorption					251:260	ruminal calcium absorption	235:260	ruminal calcium absorption	235:260	Previous studies ex vivo suggested that plant bioactive lipid compounds (PBLC) can increase ruminal calcium absorption.					
36907757	10	73	from	cows	1607:1610	arg1	d					1615:1615	d 21	1615:1618	d 21	1615:1618	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	14	74	theme	fat	2129:2131	arg1	concentrations					2111:2124	The concentrations	2107:2124	The concentrations of fat, lactose, and urea	2107:2150	The concentrations of fat, lactose, and urea, as well as somatic cell count, were not affected by treatment.					
36907757	14	74	theme	fat	2129:2131	arg1	count					2177:2181	somatic cell count	2164:2181	somatic cell count	2164:2181	The concentrations of fat, lactose, and urea, as well as somatic cell count, were not affected by treatment.					
36907757	4	75	theme	PBLC	772:775	arg1	group					787:791	PBLC treatment group	772:791	PBLC treatment group	772:791	A total of 29 BS cows and 41 HF cows were divided each into a control (CON) and PBLC treatment group.					
36907757	10	76	from	d	1615:1615	arg1	levels					1592:1597	higher sodium levels	1578:1597	higher sodium levels in PBLC cows on d 21	1578:1618	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	2	77	from	hypocalcemia	350:361	arg1	cows					407:410	postpartum dairy cows	390:410	postpartum dairy cows	390:410	Therefore, we hypothesized that PBLC feeding around calving may potentially counteract hypocalcemia and support performance in postpartum dairy cows.					
36907757	9	78	theme	HF	1351:1352	arg1	HF-CON					1362:1367	2 HF-CON	1360:1367	2 HF-CON	1360:1367	Clinical milk fever was detected only in HF cows (2 HF-CON and one HF-PBLC).					
36907757	9	78	theme	HF	1351:1352	arg1	HF-PBLC					1377:1383	one HF-PBLC	1373:1383	one HF-PBLC	1373:1383	Clinical milk fever was detected only in HF cows (2 HF-CON and one HF-PBLC).					
36907757	9	78	theme	HF	1351:1352	arg1	cows					1354:1357	HF cows	1351:1357	HF cows (2 HF-CON and one HF-PBLC)	1351:1384	Clinical milk fever was detected only in HF cows (2 HF-CON and one HF-PBLC).					
36907757	7	79	theme	Feeding	989:995	arg1	PBLC					997:1000	Feeding PBLC	989:1000	Feeding PBLC	989:1000	Feeding PBLC induced a significant breed × treatment interaction for iCa, supporting that PBLC increased iCa exclusively in HF cows; the increase was 0.03 mM over the whole period and 0.05 mM from d 1 to 3 after calving.					
36907757	15	80	theme	first	2247:2251	arg1	wk					2256:2257	the first 11 wk	2243:2257	the first 11 wk of lactation	2243:2270	The weekly milk yield over the first 11 wk of lactation was 29.5 kg/wk higher for PBLC versus CON across breeds.					
36907757	13	81	theme	first	2006:2010	arg1	day					2017:2019	the first test day	2002:2019	the first test day	2002:2019	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	7	82	theme	treatment	1032:1040	arg1	interaction					1042:1052	a significant breed × treatment interaction	1010:1052	a significant breed × treatment interaction for iCa	1010:1060	Feeding PBLC induced a significant breed × treatment interaction for iCa, supporting that PBLC increased iCa exclusively in HF cows; the increase was 0.03 mM over the whole period and 0.05 mM from d 1 to 3 after calving.					
36907757	16	83	theme	measurable	2387:2396	arg1	improvement					2398:2408	a small but measurable improvement	2375:2408	a small but measurable improvement of calcium status in HF cows	2375:2437	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	11	84	from	score	1707:1711	arg1	BS-PBLC					1716:1722	BS-PBLC	1716:1722	BS-PBLC compared with BS-CON at d 14	1716:1751	Body condition score showed no effect of treatment, except for a lower body condition score in BS-PBLC compared with BS-CON at d 14.					
36907757	12	85	theme	protein	1814:1820	arg1	yield					1822:1826	milk protein yield	1809:1826	milk protein yield	1809:1826	Dietary PBLC increased milk yield, milk fat yield, and milk protein yield at 2 consecutive dairy herd improvement test days.					
36907757	7	86	theme	HF	1113:1114	arg1	cows					1116:1119	HF cows	1113:1119	HF cows	1113:1119	Feeding PBLC induced a significant breed × treatment interaction for iCa, supporting that PBLC increased iCa exclusively in HF cows; the increase was 0.03 mM over the whole period and 0.05 mM from d 1 to 3 after calving.					
36907757	3	87	theme	Friesian	570:577	arg1	cows					584:587	hypocalcemia-susceptible Holstein Friesian (HF) cows	536:587	hypocalcemia-susceptible Holstein Friesian (HF) cows	536:587	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	1	88	theme	plant	183:187	arg1	compounds					205:213	plant bioactive lipid compounds	183:213	plant bioactive lipid compounds (PBLC)	183:220	Previous studies ex vivo suggested that plant bioactive lipid compounds (PBLC) can increase ruminal calcium absorption.					
36907757	1	88	theme	plant	183:187	arg1	PBLC					216:219	PBLC	216:219	PBLC	216:219	Previous studies ex vivo suggested that plant bioactive lipid compounds (PBLC) can increase ruminal calcium absorption.					
36907757	10	89	theme	tested	1393:1398	arg1	minerals					1406:1413	Other tested blood minerals	1387:1413	Other tested blood minerals	1387:1413	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	10	89	theme	tested	1393:1398	arg1	chloride					1432:1439	chloride	1432:1439	chloride	1432:1439	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	10	89	theme	tested	1393:1398	arg1	potassium					1446:1454	potassium	1446:1454	potassium	1446:1454	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	10	89	theme	tested	1393:1398	arg1	sodium					1424:1429	sodium	1424:1429	sodium	1424:1429	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	0	90	theme	lipid	126:130	arg1	compounds					132:140	plant bioactive lipid compounds	110:140	plant bioactive lipid compounds	110:140	Blood calcium concentration and performance in periparturient and early lactating dairy cows is influenced by plant bioactive lipid compounds.					
36907757	3	91	theme	hypocalcemia-susceptible	536:559	arg1	cows					584:587	hypocalcemia-susceptible Holstein Friesian (HF) cows	536:587	hypocalcemia-susceptible Holstein Friesian (HF) cows	536:587	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	15	92	theme	milk	2227:2230	arg1	yield					2232:2236	The weekly milk yield	2216:2236	The weekly milk yield over the first 11 wk of lactation	2216:2270	The weekly milk yield over the first 11 wk of lactation was 29.5 kg/wk higher for PBLC versus CON across breeds.					
36907757	15	92	theme	milk	2227:2230	arg1	higher					2287:2292	higher	2287:2292	higher	2287:2292	The weekly milk yield over the first 11 wk of lactation was 29.5 kg/wk higher for PBLC versus CON across breeds.					
36907757	1	93	theme	lipid	199:203	arg1	compounds					205:213	plant bioactive lipid compounds	183:213	plant bioactive lipid compounds (PBLC)	183:220	Previous studies ex vivo suggested that plant bioactive lipid compounds (PBLC) can increase ruminal calcium absorption.					
36907757	1	93	theme	lipid	199:203	arg1	PBLC					216:219	PBLC	216:219	PBLC	216:219	Previous studies ex vivo suggested that plant bioactive lipid compounds (PBLC) can increase ruminal calcium absorption.					
36907757	16	94	theme	status	2421:2426	arg1	improvement					2398:2408	a small but measurable improvement	2375:2408	a small but measurable improvement of calcium status in HF cows	2375:2437	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	3	95	theme	HF	580:581	arg1	cows					584:587	hypocalcemia-susceptible Holstein Friesian (HF) cows	536:587	hypocalcemia-susceptible Holstein Friesian (HF) cows	536:587	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	9	96	theme	milk	1319:1322	arg1	fever					1324:1328	Clinical milk fever	1310:1328	Clinical milk fever	1310:1328	Clinical milk fever was detected only in HF cows (2 HF-CON and one HF-PBLC).					
36907757	16	97	from	performance	2503:2513	arg1	breeds					2523:2528	both breeds	2518:2528	both breeds	2518:2528	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	4	98	theme	cows	724:727	arg1	total					694:698	A total	692:698	A total of 29 BS cows and 41 HF cows	692:727	A total of 29 BS cows and 41 HF cows were divided each into a control (CON) and PBLC treatment group.					
36907757	5	99	theme	g/d	831:833	arg1	PBLC					848:851	1.7 g/d menthol-rich PBLC	827:851	1.7 g/d menthol-rich PBLC from 8 d	827:860	The latter was supplemented with 1.7 g/d menthol-rich PBLC from 8 d before expected calving to 80 d postpartum.					
36907757	16	100	theme	applied	2354:2360	arg1	PBLC					2362:2365	the applied PBLC	2350:2365	the applied PBLC	2350:2365	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	12	101	theme	milk	1789:1792	arg1	yield					1798:1802	milk fat yield	1789:1802	milk fat yield	1789:1802	Dietary PBLC increased milk yield, milk fat yield, and milk protein yield at 2 consecutive dairy herd improvement test days.					
36907757	3	102	theme	PBLC	481:484	arg1	feeding					486:492	PBLC feeding	481:492	PBLC feeding	481:492	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	0	103	theme	lactating	72:80	arg1	cows					88:91	periparturient and early lactating dairy cows	47:91	periparturient and early lactating dairy cows	47:91	Blood calcium concentration and performance in periparturient and early lactating dairy cows is influenced by plant bioactive lipid compounds.					
36907757	11	104	theme	body	1692:1695	arg1	score					1707:1711	a lower body condition score	1684:1711	a lower body condition score in BS-PBLC compared with BS-CON at d 14	1684:1751	Body condition score showed no effect of treatment, except for a lower body condition score in BS-PBLC compared with BS-CON at d 14.					
36907757	1	105	theme	Previous	143:150	arg1	studies					152:158	Previous studies	143:158	Previous studies ex vivo	143:166	Previous studies ex vivo suggested that plant bioactive lipid compounds (PBLC) can increase ruminal calcium absorption.					
36907757	10	106	theme	Other	1387:1391	arg1	minerals					1406:1413	Other tested blood minerals	1387:1413	Other tested blood minerals	1387:1413	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	10	106	theme	Other	1387:1391	arg1	chloride					1432:1439	chloride	1432:1439	chloride	1432:1439	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	10	106	theme	Other	1387:1391	arg1	potassium					1446:1454	potassium	1446:1454	potassium	1446:1454	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	10	106	theme	Other	1387:1391	arg1	sodium					1424:1429	sodium	1424:1429	sodium	1424:1429	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	12	107	theme	Dietary	1754:1760	arg1	PBLC					1762:1765	Dietary PBLC	1754:1765	Dietary PBLC	1754:1765	Dietary PBLC increased milk yield, milk fat yield, and milk protein yield at 2 consecutive dairy herd improvement test days.					
36907757	1	108	theme	ex	160:161	arg1	studies					152:158	Previous studies	143:158	Previous studies ex vivo	143:166	Previous studies ex vivo suggested that plant bioactive lipid compounds (PBLC) can increase ruminal calcium absorption.					
36907757	16	109	theme	HF	2431:2432	arg1	cows					2434:2437	HF cows	2431:2437	HF cows	2431:2437	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	12	110	theme	consecutive	1833:1843	arg1	days					1873:1876	2 consecutive dairy herd improvement test days	1831:1876	2 consecutive dairy herd improvement test days	1831:1876	Dietary PBLC increased milk yield, milk fat yield, and milk protein yield at 2 consecutive dairy herd improvement test days.					
36907757	4	111	theme	BS	706:707	arg1	cows					709:712	29 BS cows	703:712	29 BS cows	703:712	A total of 29 BS cows and 41 HF cows were divided each into a control (CON) and PBLC treatment group.					
36907757	0	112	theme	plant	110:114	arg1	compounds					132:140	plant bioactive lipid compounds	110:140	plant bioactive lipid compounds	110:140	Blood calcium concentration and performance in periparturient and early lactating dairy cows is influenced by plant bioactive lipid compounds.					
36907757	0	113	theme	calcium	6:12	arg1	concentration					14:26	Blood calcium concentration	0:26	Blood calcium concentration	0:26	Blood calcium concentration and performance in periparturient and early lactating dairy cows is influenced by plant bioactive lipid compounds.					
36907757	6	114	theme	blood	959:963	arg1	minerals					965:972	blood minerals	959:972	blood minerals	959:972	Milk yield and composition, body condition score and blood minerals were measured.					
36907757	8	115	theme	Subclinical	1210:1220	arg1	hypocalcemia					1222:1233	Subclinical hypocalcemia	1210:1233	Subclinical hypocalcemia	1210:1233	Subclinical hypocalcemia was seen in one BS-CON and 8 HF-CON cows and 2 BS-PBLC and 4 HF-PBLC cows.					
36907757	3	116	theme	study	442:446	arg1	aim					431:433	The corresponding aim	413:433	The corresponding aim of the study	413:446	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	12	117	theme	dairy	1845:1849	arg1	days					1873:1876	2 consecutive dairy herd improvement test days	1831:1876	2 consecutive dairy herd improvement test days	1831:1876	Dietary PBLC increased milk yield, milk fat yield, and milk protein yield at 2 consecutive dairy herd improvement test days.					
36907757	13	118	theme	energy-corrected	1925:1940	arg1	yield					1947:1951	energy-corrected milk yield	1925:1951	energy-corrected milk yield	1925:1951	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	6	119	theme	body	934:937	arg1	score					949:953	body condition score	934:953	body condition score	934:953	Milk yield and composition, body condition score and blood minerals were measured.					
36907757	10	120	theme	PBLC	1602:1605	arg1	cows					1607:1610	PBLC cows	1602:1610	PBLC cows on d 21	1602:1618	Other tested blood minerals, such as sodium, chloride, and potassium, as well as blood glucose, were neither affected by PBLC feeding nor breed, nor were their 2-way interactions, except for higher sodium levels in PBLC cows on d 21.					
36907757	12	121	theme	test	1868:1871	arg1	days					1873:1876	2 consecutive dairy herd improvement test days	1831:1876	2 consecutive dairy herd improvement test days	1831:1876	Dietary PBLC increased milk yield, milk fat yield, and milk protein yield at 2 consecutive dairy herd improvement test days.					
36907757	7	122	theme	×	1030:1030	arg1	interaction					1042:1052	a significant breed × treatment interaction	1010:1052	a significant breed × treatment interaction for iCa	1010:1060	Feeding PBLC induced a significant breed × treatment interaction for iCa, supporting that PBLC increased iCa exclusively in HF cows; the increase was 0.03 mM over the whole period and 0.05 mM from d 1 to 3 after calving.					
36907757	3	123	theme	corresponding	417:429	arg1	aim					431:433	The corresponding aim	413:433	The corresponding aim of the study	413:446	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	8	124	theme	BS-CON	1251:1256	arg1	cows					1271:1274	one BS-CON and 8 HF-CON cows	1247:1274	one BS-CON and 8 HF-CON cows	1247:1274	Subclinical hypocalcemia was seen in one BS-CON and 8 HF-CON cows and 2 BS-PBLC and 4 HF-PBLC cows.					
36907757	3	125	from	effect	471:476	arg1	performance					655:665	milk performance	650:665	milk performance	650:665	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	3	125	from	effect	471:476	arg1	minerals					503:510	blood minerals	497:510	blood minerals in Brown Swiss (BS)	497:530	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	3	125	from	effect	471:476	arg1	BS					528:529	BS	528:529	BS	528:529	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	3	126	theme	blood	497:501	arg1	BS					528:529	BS	528:529	BS	528:529	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	3	126	theme	blood	497:501	arg1	minerals					503:510	blood minerals	497:510	blood minerals in Brown Swiss (BS)	497:530	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	16	127	from	improvement	2398:2408	arg1	cows					2434:2437	HF cows	2431:2437	HF cows	2431:2437	It is concluded that the applied PBLC induced a small but measurable improvement of calcium status in HF cows in the study period and had additional positive effects on milk performance in both breeds.					
36907757	13	128	theme	×	1905:1905	arg1	interactions					1911:1922	treatment × day interactions	1895:1922	treatment × day interactions	1895:1922	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	14	129	theme	urea	2147:2150	arg1	concentrations					2111:2124	The concentrations	2107:2124	The concentrations of fat, lactose, and urea	2107:2150	The concentrations of fat, lactose, and urea, as well as somatic cell count, were not affected by treatment.					
36907757	14	129	theme	urea	2147:2150	arg1	count					2177:2181	somatic cell count	2164:2181	somatic cell count	2164:2181	The concentrations of fat, lactose, and urea, as well as somatic cell count, were not affected by treatment.					
36907757	11	130	from	d	1748:1748	arg1	BS-CON					1738:1743	BS-CON	1738:1743	BS-CON at d 14	1738:1751	Body condition score showed no effect of treatment, except for a lower body condition score in BS-PBLC compared with BS-CON at d 14.					
36907757	4	131	theme	HF	721:722	arg1	cows					724:727	41 HF cows	718:727	41 HF cows	718:727	A total of 29 BS cows and 41 HF cows were divided each into a control (CON) and PBLC treatment group.					
36907757	3	132	theme	feeding	486:492	arg1	effect					471:476	the effect	467:476	the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance	467:665	The corresponding aim of the study was to investigate the effect of PBLC feeding on blood minerals in Brown Swiss (BS) and hypocalcemia-susceptible Holstein Friesian (HF) cows during the period from d -2 to 28 relative to calving and on milk performance until d 80 of lactation.					
36907757	9	133	theme	Clinical	1310:1317	arg1	fever					1324:1328	Clinical milk fever	1310:1328	Clinical milk fever	1310:1328	Clinical milk fever was detected only in HF cows (2 HF-CON and one HF-PBLC).					
36907757	13	134	theme	milk	2031:2034	arg1	concentration					2044:2056	milk protein concentration	2031:2056	milk protein concentration	2031:2056	As indicated by treatment × day interactions, energy-corrected milk yield and milk lactose yield were increased by PBLC on the first test day only, and milk protein concentration decreased from test d 1 to test d 2 in CON only.					
36907757	7	135	from	d	1186:1186	arg1	mM					1178:1179	0.05 mM	1173:1179	0.05 mM from d 1 to 3	1173:1193	Feeding PBLC induced a significant breed × treatment interaction for iCa, supporting that PBLC increased iCa exclusively in HF cows; the increase was 0.03 mM over the whole period and 0.05 mM from d 1 to 3 after calving.					
36907757	7	135	from	d	1186:1186	arg1	increase					1126:1133	the increase	1122:1133	the increase	1122:1133	Feeding PBLC induced a significant breed × treatment interaction for iCa, supporting that PBLC increased iCa exclusively in HF cows; the increase was 0.03 mM over the whole period and 0.05 mM from d 1 to 3 after calving.					
36907757	7	135	from	d	1186:1186	arg1	mM					1144:1145	0.03 mM	1139:1145	0.03 mM over the whole period	1139:1167	Feeding PBLC induced a significant breed × treatment interaction for iCa, supporting that PBLC increased iCa exclusively in HF cows; the increase was 0.03 mM over the whole period and 0.05 mM from d 1 to 3 after calving.					
36907757	12	136	theme	herd	1851:1854	arg1	days					1873:1876	2 consecutive dairy herd improvement test days	1831:1876	2 consecutive dairy herd improvement test days	1831:1876	Dietary PBLC increased milk yield, milk fat yield, and milk protein yield at 2 consecutive dairy herd improvement test days.					
36907757	11	137	theme	Body	1621:1624	arg1	score					1636:1640	Body condition score	1621:1640	Body condition score	1621:1640	Body condition score showed no effect of treatment, except for a lower body condition score in BS-PBLC compared with BS-CON at d 14.					
36907757	1	138	theme	ruminal	235:241	arg1	absorption					251:260	ruminal calcium absorption	235:260	ruminal calcium absorption	235:260	Previous studies ex vivo suggested that plant bioactive lipid compounds (PBLC) can increase ruminal calcium absorption.					
36715114	12	0	theme	diversity	1609:1617	arg1	indices					1619:1625	diversity indices	1609:1625	diversity indices	1609:1625	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	3	1	theme	hindgut	494:500	arg1	microbiota					502:511	the hindgut microbiota	490:511	the hindgut microbiota to promote health and prevent disease	490:549	Synbiotics, supplements that combine probiotic microorganisms with prebiotic ingredients, are a potential means of influencing the hindgut microbiota to promote health and prevent disease.					
36715114	15	2	theme	prebiotic	2141:2149	arg1	effect					2151:2156	a potential prebiotic effect	2129:2156	a potential prebiotic effect of the added supplement	2129:2180	Although no changes within the microbiome were apparent, the consistent changes in metabolites indicates a potential prebiotic effect of the added supplement and merits further exploration.					
36715114	4	3	theme	study	581:585	arg1	objective					556:564	The objective	552:564	The objective of the current study	552:585	The objective of the current study was to evaluate the influence of an equine probiotic/prebiotic supplement on characteristics of the microbiota and metabolite production in vitro.					
36715114	7	4	theme	anaerobic	1045:1053	arg1	n=15					1062:1065	n=15	1062:1065	n=15	1062:1065	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	7	4	theme	anaerobic	1045:1053	arg1	cecal					1055:1059	anaerobic cecal	1045:1059	anaerobic cecal	1045:1059	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	12	5	from	48h	1588:1590	arg1	treatment					1568:1576	treatment	1568:1576	treatment at 24h or 48h	1568:1590	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	12	5	from	48h	1588:1590	arg1	effect					1558:1563	no effect	1555:1563	no effect of treatment at 24h or 48h on alpha or beta diversity indices	1555:1625	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	4	6	theme	supplement	650:659	arg1	influence					607:615	the influence	603:615	the influence of an equine probiotic/prebiotic supplement on characteristics of the microbiota and metabolite production	603:722	The objective of the current study was to evaluate the influence of an equine probiotic/prebiotic supplement on characteristics of the microbiota and metabolite production in vitro.					
36715114	11	7	theme	statistical	1503:1513	arg1	analyses					1515:1522	All statistical analyses	1499:1522	All statistical analyses	1499:1522	All statistical analyses were run in SAS 9.4.					
36715114	7	8	theme	fluid	1038:1042	arg1	ml					1026:1027	One hundred ml	1014:1027	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6)	1014:1108	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	1	9	theme	equine	172:177	arg1	hindgut					179:185	the equine hindgut	168:185	the equine hindgut	168:185	The microbes that reside within the equine hindgut create a complex and dynamic ecosystem.					
36715114	14	10	theme	fermentable	1982:1992	arg1	substrate					1994:2002	the provided fermentable substrate	1969:2002	the provided fermentable substrate	1969:2002	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	0	11	theme	fecal	113:117	arg1	slurry					119:124	fecal slurry	113:124	fecal slurry in vitro	113:133	The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro.					
36715114	14	12	with	profile	1832:1838	arg1	impact					1860:1865	no discernable impact	1845:1865	no discernable impact on the microbiota of these in vitro systems	1845:1909	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	6	13	dep	fermenters	907:916	arg1	n=11					936:939	n=11	936:939	n=11	936:939	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	6	13	dep	fermenters	907:916	arg1	cecal					929:933	chemostat cecal	919:933	chemostat cecal	919:933	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	12	14	from	effect	1558:1563	arg1	48h					1588:1590	48h	1588:1590	48h	1588:1590	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	12	14	from	effect	1558:1563	arg1	24h					1581:1583	24h	1581:1583	24h	1581:1583	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	12	14	from	effect	1558:1563	arg1	beta					1604:1607	beta	1604:1607	beta	1604:1607	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	12	14	from	effect	1558:1563	arg1	alpha					1595:1599	alpha	1595:1599	alpha	1595:1599	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	4	15	theme	equine	623:628	arg1	supplement					650:659	an equine probiotic/prebiotic supplement	620:659	an equine probiotic/prebiotic supplement	620:659	The objective of the current study was to evaluate the influence of an equine probiotic/prebiotic supplement on characteristics of the microbiota and metabolite production in vitro.					
36715114	1	16	theme	complex	196:202	arg1	ecosystem					216:224	a complex and dynamic ecosystem	194:224	a complex and dynamic ecosystem	194:224	The microbes that reside within the equine hindgut create a complex and dynamic ecosystem.					
36715114	6	17	theme	chemostat	919:927	arg1	n=11					936:939	n=11	936:939	n=11	936:939	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	6	17	theme	chemostat	919:927	arg1	cecal					929:933	chemostat cecal	919:933	chemostat cecal	919:933	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	4	18	theme	production	713:722	arg1	characteristics					664:678	characteristics	664:678	characteristics of the microbiota and metabolite production	664:722	The objective of the current study was to evaluate the influence of an equine probiotic/prebiotic supplement on characteristics of the microbiota and metabolite production in vitro.					
36715114	7	19	dep	fluid	1038:1042	arg1	n=15					1062:1065	n=15	1062:1065	n=15	1062:1065	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	7	19	dep	fluid	1038:1042	arg1	cecal					1055:1059	anaerobic cecal	1045:1059	anaerobic cecal	1045:1059	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	14	20	from	effect	1789:1794	arg1	profile					1832:1838	the metabolic profile	1818:1838	the metabolic profile with no discernable impact on the microbiota of these in vitro systems	1818:1909	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	14	21	dep	in	1894:1895	arg1	vitro					1897:1901	vitro	1897:1901	vitro	1897:1901	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	7	22	theme	vessel	1220:1225	arg1	establishment					1227:1239	vessel establishment	1220:1239	vessel establishment	1220:1239	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	15	23	theme	supplement	2171:2180	arg1	merits					2186:2191	merits	2186:2191	merits	2186:2191	Although no changes within the microbiome were apparent, the consistent changes in metabolites indicates a potential prebiotic effect of the added supplement and merits further exploration.					
36715114	15	23	theme	supplement	2171:2180	arg1	effect					2151:2156	a potential prebiotic effect	2129:2156	a potential prebiotic effect of the added supplement	2129:2180	Although no changes within the microbiome were apparent, the consistent changes in metabolites indicates a potential prebiotic effect of the added supplement and merits further exploration.					
36715114	2	24	theme	hindgut	238:244	arg1	such					302:305	such	302:305	such	302:305	The equine hindgut microbiota is intimately associated with health and, as such, represents an area which can be beneficially modified.					
36715114	2	24	theme	hindgut	238:244	arg1	microbiota					246:255	The equine hindgut microbiota	227:255	The equine hindgut microbiota	227:255	The equine hindgut microbiota is intimately associated with health and, as such, represents an area which can be beneficially modified.					
36715114	8	25	theme	incubation	1319:1328	arg1	48h					1312:1314	48h	1312:1314	48h	1312:1314	Samples were taken from vessels at vessel establishment (0h), 24h, or 48h of incubation.					
36715114	8	25	theme	incubation	1319:1328	arg1	0h					1299:1300	0h	1299:1300	0h	1299:1300	Samples were taken from vessels at vessel establishment (0h), 24h, or 48h of incubation.					
36715114	8	25	theme	incubation	1319:1328	arg1	24h					1304:1306	24h	1304:1306	24h	1304:1306	Samples were taken from vessels at vessel establishment (0h), 24h, or 48h of incubation.					
36715114	8	25	theme	incubation	1319:1328	arg1	establishment					1284:1296	vessel establishment	1277:1296	vessel establishment (0h)	1277:1301	Samples were taken from vessels at vessel establishment (0h), 24h, or 48h of incubation.					
36715114	12	26	from	24h	1581:1583	arg1	treatment					1568:1576	treatment	1568:1576	treatment at 24h or 48h	1568:1590	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	12	26	from	24h	1581:1583	arg1	effect					1558:1563	no effect	1555:1563	no effect of treatment at 24h or 48h on alpha or beta diversity indices	1555:1625	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	13	27	theme	untreated	1742:1750	arg1	vessels					1752:1758	untreated vessels	1742:1758	untreated vessels in all methods	1742:1773	Acetate, propionate, and butyrate were higher in treated compared to untreated vessels in all methods.					
36715114	5	28	theme	fecal	757:761	arg1	material					763:770	fecal material	757:770	fecal material	757:770	Equine cecal fluid and fecal material were collected from an abattoir in QC, CAN.					
36715114	9	29	theme	region	1361:1366	arg1	sequencing					1340:1349	Illumina sequencing	1331:1349	Illumina sequencing of the V4 region of the 16S rRNA gene	1331:1387	Illumina sequencing of the V4 region of the 16S rRNA gene and bioinformatics were performed for microbiome analysis.					
36715114	9	29	theme	region	1361:1366	arg1	bioinformatics					1393:1406	bioinformatics	1393:1406	bioinformatics	1393:1406	Illumina sequencing of the V4 region of the 16S rRNA gene and bioinformatics were performed for microbiome analysis.					
36715114	7	30	dep	slurry	1080:1085	arg1	n=6					1105:1107	n=6	1105:1107	n=6	1105:1107	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	7	30	dep	slurry	1080:1085	arg1	fecal					1098:1102	anaerobic fecal	1088:1102	anaerobic fecal	1088:1102	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	0	31	theme	equine	91:96	arg1	fluid					104:108	equine cecal fluid	91:108	equine cecal fluid	91:108	The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro.					
36715114	9	32	theme	rRNA	1379:1382	arg1	gene					1384:1387	the 16S rRNA gene	1371:1387	the 16S rRNA gene	1371:1387	Illumina sequencing of the V4 region of the 16S rRNA gene and bioinformatics were performed for microbiome analysis.					
36715114	14	33	theme	inoculum	1921:1928	arg1	viability					1938:1946	inoculum microbe viability	1921:1946	inoculum microbe viability	1921:1946	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	0	34	theme	fluid	104:108	arg1	parameters					77:86	microbial and metabolic parameters	53:86	microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro	53:133	The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro.					
36715114	14	35	theme	metabolic	1822:1830	arg1	profile					1832:1838	the metabolic profile	1818:1838	the metabolic profile with no discernable impact on the microbiota of these in vitro systems	1818:1909	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	14	36	theme	systems	1903:1909	arg1	microbiota					1874:1883	the microbiota	1870:1883	the microbiota of these in vitro systems	1870:1909	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	0	37	theme	metabolic	67:75	arg1	parameters					77:86	microbial and metabolic parameters	53:86	microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro	53:133	The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro.					
36715114	3	38	theme	probiotic	400:408	arg1	microorganisms					410:423	probiotic microorganisms	400:423	probiotic microorganisms	400:423	Synbiotics, supplements that combine probiotic microorganisms with prebiotic ingredients, are a potential means of influencing the hindgut microbiota to promote health and prevent disease.					
36715114	14	39	theme	in	1894:1895	arg1	systems					1903:1909	these in vitro systems	1888:1909	these in vitro systems	1888:1909	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	7	40	theme	fecal	1074:1078	arg1	slurry					1080:1085	5% fecal slurry	1071:1085	5% fecal slurry (anaerobic fecal, n=6)	1071:1108	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	15	41	theme	consistent	2085:2094	arg1	changes					2096:2102	the consistent changes	2081:2102	the consistent changes in metabolites	2081:2117	Although no changes within the microbiome were apparent, the consistent changes in metabolites indicates a potential prebiotic effect of the added supplement and merits further exploration.					
36715114	15	42	dep	effect	2151:2156	arg1	exploration					2201:2211	further exploration	2193:2211	further exploration	2193:2211	Although no changes within the microbiome were apparent, the consistent changes in metabolites indicates a potential prebiotic effect of the added supplement and merits further exploration.					
36715114	0	43	theme	probiotic/prebiotic	19:37	arg1	supplement					39:48	a probiotic/prebiotic supplement	17:48	a probiotic/prebiotic supplement	17:48	The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro.					
36715114	7	44	theme	5	1071:1071	arg1	%					1072:1072	%	1072:1072	%	1072:1072	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	6	45	theme	cecal	835:839	arg1	fluid					841:845	cecal fluid	835:845	cecal fluid	835:845	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	14	46	theme	consistent	1778:1787	arg1	effect					1789:1794	A consistent effect	1776:1794	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems	1776:1909	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	12	47	theme	limited	1631:1637	arg1	differences					1649:1659	limited taxonomic differences	1631:1659	limited taxonomic differences	1631:1659	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	6	48	theme	supplement	979:988	arg1	control					958:964	control	958:964	control	958:964	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	6	48	theme	supplement	979:988	arg1	0.44g					970:974	0.44g	970:974	0.44g of supplement added at 12h intervals	970:1011	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	6	48	theme	supplement	979:988	arg1	0g					954:955	0g	954:955	0g (control)	954:965	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	8	49	theme	vessel	1277:1282	arg1	0h					1299:1300	0h	1299:1300	0h	1299:1300	Samples were taken from vessels at vessel establishment (0h), 24h, or 48h of incubation.					
36715114	8	49	theme	vessel	1277:1282	arg1	establishment					1284:1296	vessel establishment	1277:1296	vessel establishment (0h)	1277:1301	Samples were taken from vessels at vessel establishment (0h), 24h, or 48h of incubation.					
36715114	14	50	from	impact	1860:1865	arg1	microbiota					1874:1883	the microbiota	1870:1883	the microbiota of these in vitro systems	1870:1909	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	3	51	theme	potential	459:467	arg1	Synbiotics					363:372	Synbiotics	363:372	Synbiotics	363:372	Synbiotics, supplements that combine probiotic microorganisms with prebiotic ingredients, are a potential means of influencing the hindgut microbiota to promote health and prevent disease.					
36715114	3	51	theme	potential	459:467	arg1	means					469:473	a potential means	457:473	a potential means of influencing the hindgut microbiota to promote health and prevent disease	457:549	Synbiotics, supplements that combine probiotic microorganisms with prebiotic ingredients, are a potential means of influencing the hindgut microbiota to promote health and prevent disease.					
36715114	6	52	theme	batch	901:905	arg1	fermenters					907:916	batch fermenters	901:916	batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals	901:1011	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	15	53	theme	potential	2131:2139	arg1	effect					2151:2156	a potential prebiotic effect	2129:2156	a potential prebiotic effect of the added supplement	2129:2180	Although no changes within the microbiome were apparent, the consistent changes in metabolites indicates a potential prebiotic effect of the added supplement and merits further exploration.					
36715114	7	54	theme	anaerobic	1132:1140	arg1	chamber					1142:1148	an anaerobic chamber	1129:1148	an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment	1129:1239	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	7	55	with	chamber	1142:1148	arg1	0.356g					1178:1183	0.356g	1178:1183	0.356g of supplement added at the time of vessel establishment	1178:1239	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	7	55	with	chamber	1142:1148	arg1	0g					1162:1163	0g	1162:1163	0g (control)	1162:1173	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	7	55	with	chamber	1142:1148	arg1	control					1166:1172	control	1166:1172	control	1166:1172	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	6	56	theme	chemostat	869:877	arg1	vessels					879:885	chemostat vessels	869:885	chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals	869:1011	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	4	57	theme	current	573:579	arg1	study					581:585	the current study	569:585	the current study	569:585	The objective of the current study was to evaluate the influence of an equine probiotic/prebiotic supplement on characteristics of the microbiota and metabolite production in vitro.					
36715114	7	58	theme	cecal	1032:1036	arg1	fluid					1038:1042	cecal fluid	1032:1042	cecal fluid (anaerobic cecal, n=15)	1032:1066	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	15	59	from	changes	2096:2102	arg1	metabolites					2107:2117	metabolites	2107:2117	metabolites	2107:2117	Although no changes within the microbiome were apparent, the consistent changes in metabolites indicates a potential prebiotic effect of the added supplement and merits further exploration.					
36715114	12	60	theme	treatment	1568:1576	arg1	effect					1558:1563	no effect	1555:1563	no effect of treatment at 24h or 48h on alpha or beta diversity indices	1555:1625	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	4	61	theme	probiotic/prebiotic	630:648	arg1	supplement					650:659	an equine probiotic/prebiotic supplement	620:659	an equine probiotic/prebiotic supplement	620:659	The objective of the current study was to evaluate the influence of an equine probiotic/prebiotic supplement on characteristics of the microbiota and metabolite production in vitro.					
36715114	10	62	theme	Metabolite	1448:1457	arg1	data					1459:1462	Metabolite data	1448:1462	Metabolite data	1448:1462	Metabolite data was obtained via NMR spectroscopy.					
36715114	6	63	with	fermenters	907:916	arg1	control					958:964	control	958:964	control	958:964	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	6	63	with	fermenters	907:916	arg1	0.44g					970:974	0.44g	970:974	0.44g of supplement added at 12h intervals	970:1011	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	6	63	with	fermenters	907:916	arg1	0g					954:955	0g	954:955	0g (control)	954:965	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	14	64	theme	substrate	1994:2002	arg1	viability					1938:1946	inoculum microbe viability	1921:1946	inoculum microbe viability	1921:1946	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	14	64	theme	substrate	1994:2002	arg1	utilization					1954:1964	a utilization	1952:1964	a utilization of the provided fermentable substrate	1952:2002	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	0	65	theme	slurry	119:124	arg1	parameters					77:86	microbial and metabolic parameters	53:86	microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro	53:133	The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro.					
36715114	2	66	dep	associated	271:280	arg1	represents					308:317	represents	308:317	represents an area which can be beneficially modified	308:360	The equine hindgut microbiota is intimately associated with health and, as such, represents an area which can be beneficially modified.					
36715114	14	67	theme	provided	1973:1980	arg1	substrate					1994:2002	the provided fermentable substrate	1969:2002	the provided fermentable substrate	1969:2002	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	4	68	from	influence	607:615	arg1	characteristics					664:678	characteristics	664:678	characteristics of the microbiota and metabolite production	664:722	The objective of the current study was to evaluate the influence of an equine probiotic/prebiotic supplement on characteristics of the microbiota and metabolite production in vitro.					
36715114	9	69	theme	Illumina	1331:1338	arg1	sequencing					1340:1349	Illumina sequencing	1331:1349	Illumina sequencing of the V4 region of the 16S rRNA gene	1331:1387	Illumina sequencing of the V4 region of the 16S rRNA gene and bioinformatics were performed for microbiome analysis.					
36715114	4	70	theme	metabolite	702:711	arg1	production					713:722	the microbiota and metabolite production	683:722	production	713:722	The objective of the current study was to evaluate the influence of an equine probiotic/prebiotic supplement on characteristics of the microbiota and metabolite production in vitro.					
36715114	15	71	theme	further	2193:2199	arg1	exploration					2201:2211	further exploration	2193:2211	further exploration	2193:2211	Although no changes within the microbiome were apparent, the consistent changes in metabolites indicates a potential prebiotic effect of the added supplement and merits further exploration.					
36715114	13	72	from	vessels	1752:1758	arg1	methods					1767:1773	all methods	1763:1773	all methods	1763:1773	Acetate, propionate, and butyrate were higher in treated compared to untreated vessels in all methods.					
36715114	7	73	theme	establishment	1227:1239	arg1	time					1212:1215	the time	1208:1215	the time of vessel establishment	1208:1239	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	6	74	used	used	851:854	arg2	ml					829:830	Five hundred ml	816:830	Five hundred ml of cecal fluid	816:845	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	2	75	theme	equine	231:236	arg1	such					302:305	such	302:305	such	302:305	The equine hindgut microbiota is intimately associated with health and, as such, represents an area which can be beneficially modified.					
36715114	2	75	theme	equine	231:236	arg1	microbiota					246:255	The equine hindgut microbiota	227:255	The equine hindgut microbiota	227:255	The equine hindgut microbiota is intimately associated with health and, as such, represents an area which can be beneficially modified.					
36715114	9	76	theme	V4	1358:1359	arg1	region					1361:1366	the V4 region	1354:1366	the V4 region of the 16S rRNA gene	1354:1387	Illumina sequencing of the V4 region of the 16S rRNA gene and bioinformatics were performed for microbiome analysis.					
36715114	12	77	dep	alpha	1595:1599	arg1	indices					1619:1625	diversity indices	1609:1625	diversity indices	1609:1625	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	5	78	theme	Equine	734:739	arg1	fluid					747:751	Equine cecal fluid	734:751	Equine cecal fluid	734:751	Equine cecal fluid and fecal material were collected from an abattoir in QC, CAN.					
36715114	5	79	from	abattoir	795:802	arg1	QC					807:808	QC	807:808	QC	807:808	Equine cecal fluid and fecal material were collected from an abattoir in QC, CAN.					
36715114	0	80	from	influence	4:12	arg1	parameters					77:86	microbial and metabolic parameters	53:86	microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro	53:133	The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro.					
36715114	4	81	theme	microbiota	687:696	arg1	production					713:722	the microbiota and metabolite production	683:722	production	713:722	The objective of the current study was to evaluate the influence of an equine probiotic/prebiotic supplement on characteristics of the microbiota and metabolite production in vitro.					
36715114	0	82	theme	cecal	98:102	arg1	fluid					104:108	equine cecal fluid	91:108	equine cecal fluid	91:108	The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro.					
36715114	9	83	theme	16S	1375:1377	arg1	rRNA					1379:1382	16S rRNA	1375:1382	the 16S rRNA gene	1371:1387	Illumina sequencing of the V4 region of the 16S rRNA gene and bioinformatics were performed for microbiome analysis.					
36715114	14	84	theme	microbe	1930:1936	arg1	viability					1938:1946	inoculum microbe viability	1921:1946	inoculum microbe viability	1921:1946	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	7	85	theme	anaerobic	1088:1096	arg1	n=6					1105:1107	n=6	1105:1107	n=6	1105:1107	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	7	85	theme	anaerobic	1088:1096	arg1	fecal					1098:1102	anaerobic fecal	1088:1102	anaerobic fecal	1088:1102	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	9	86	theme	gene	1384:1387	arg1	region					1361:1366	the V4 region	1354:1366	the V4 region of the 16S rRNA gene	1354:1387	Illumina sequencing of the V4 region of the 16S rRNA gene and bioinformatics were performed for microbiome analysis.					
36715114	15	87	theme	added	2165:2169	arg1	supplement					2171:2180	the added supplement	2161:2180	the added supplement	2161:2180	Although no changes within the microbiome were apparent, the consistent changes in metabolites indicates a potential prebiotic effect of the added supplement and merits further exploration.					
36715114	3	88	theme	prebiotic	430:438	arg1	ingredients					440:450	prebiotic ingredients	430:450	prebiotic ingredients	430:450	Synbiotics, supplements that combine probiotic microorganisms with prebiotic ingredients, are a potential means of influencing the hindgut microbiota to promote health and prevent disease.					
36715114	7	89	theme	slurry	1080:1085	arg1	ml					1026:1027	One hundred ml	1014:1027	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6)	1014:1108	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	7	90	theme	supplement	1188:1197	arg1	0.356g					1178:1183	0.356g	1178:1183	0.356g of supplement added at the time of vessel establishment	1178:1239	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	7	90	theme	supplement	1188:1197	arg1	0g					1162:1163	0g	1162:1163	0g (control)	1162:1173	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	7	90	theme	supplement	1188:1197	arg1	control					1166:1172	control	1166:1172	control	1166:1172	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	10	91	theme	NMR	1481:1483	arg1	spectroscopy					1485:1496	NMR spectroscopy	1481:1496	NMR spectroscopy	1481:1496	Metabolite data was obtained via NMR spectroscopy.					
36715114	7	92	theme	%	1072:1072	arg1	slurry					1080:1085	5% fecal slurry	1071:1085	5% fecal slurry (anaerobic fecal, n=6)	1071:1108	One hundred ml of cecal fluid (anaerobic cecal, n=15) or 5% fecal slurry (anaerobic fecal, n=6) were maintained in an anaerobic chamber with either 0g (control) or 0.356g of supplement added at the time of vessel establishment.					
36715114	14	93	theme	supplementation	1799:1813	arg1	effect					1789:1794	A consistent effect	1776:1794	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems	1776:1909	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	6	94	theme	12h	999:1001	arg1	intervals					1003:1011	12h intervals	999:1011	12h intervals	999:1011	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	11	95	from	run	1529:1531	arg1	SAS					1536:1538	SAS 9.4	1536:1542	SAS 9.4	1536:1542	All statistical analyses were run in SAS 9.4.					
36715114	0	96	theme	supplement	39:48	arg1	influence					4:12	The influence	0:12	The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro.	0:134	The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro.					
36715114	6	97	theme	fluid	841:845	arg1	ml					829:830	Five hundred ml	816:830	Five hundred ml of cecal fluid	816:845	Five hundred ml of cecal fluid was used to inoculate chemostat vessels maintained as batch fermenters (chemostat cecal, n=11) with either 0g (control) or 0.44g of supplement added at 12h intervals.					
36715114	9	98	theme	microbiome	1427:1436	arg1	analysis					1438:1445	microbiome analysis	1427:1445	microbiome analysis	1427:1445	Illumina sequencing of the V4 region of the 16S rRNA gene and bioinformatics were performed for microbiome analysis.					
36715114	0	99	theme	microbial	53:61	arg1	parameters					77:86	microbial and metabolic parameters	53:86	microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro	53:133	The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro.					
36715114	12	100	theme	taxonomic	1639:1647	arg1	differences					1649:1659	limited taxonomic differences	1631:1659	limited taxonomic differences	1631:1659	There was no effect of treatment at 24h or 48h on alpha or beta diversity indices and limited taxonomic differences were noted.					
36715114	5	101	theme	cecal	741:745	arg1	fluid					747:751	Equine cecal fluid	734:751	Equine cecal fluid	734:751	Equine cecal fluid and fecal material were collected from an abattoir in QC, CAN.					
36715114	14	102	theme	discernable	1848:1858	arg1	impact					1860:1865	no discernable impact	1845:1865	no discernable impact on the microbiota of these in vitro systems	1845:1909	A consistent effect of supplementation on the metabolic profile with no discernable impact on the microbiota of these in vitro systems indicates inoculum microbe viability and a utilization of the provided fermentable substrate within the systems.					
36715114	1	103	theme	dynamic	208:214	arg1	ecosystem					216:224	a complex and dynamic ecosystem	194:224	a complex and dynamic ecosystem	194:224	The microbes that reside within the equine hindgut create a complex and dynamic ecosystem.					
36354485	0	0	theme	Pulse	123:127	arg1	Voltammetry					129:139	Differential Pulse Voltammetry	110:139	Differential Pulse Voltammetry	110:139	Efficient Detection of 2,6-Dinitrophenol with Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites by Differential Pulse Voltammetry.					
36354485	1	1	theme	glassy	418:423	arg1	GCE					443:445	GCE	443:445	GCE	443:445	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	1	1	theme	glassy	418:423	arg1	electrode					432:440	a glassy carbon electrode	416:440	a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor	416:532	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	6	2	theme	healthcare	1506:1515	arg1	fields					1517:1522	environmental and healthcare fields	1488:1522	environmental and healthcare fields	1488:1522	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	1	3	theme	photoreduction	194:207	arg1	technique					209:217	a photoreduction technique	192:217	a photoreduction technique	192:217	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	3	4	theme	differential	887:898	arg1	approach					924:931	the differential pulse voltammetry (DPV) approach	883:931	the differential pulse voltammetry (DPV) approach	883:931	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	0	5	theme	Differential	110:121	arg1	Voltammetry					129:139	Differential Pulse Voltammetry	110:139	Differential Pulse Voltammetry	110:139	Efficient Detection of 2,6-Dinitrophenol with Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites by Differential Pulse Voltammetry.					
36354485	1	6	theme	carbon	425:430	arg1	GCE					443:445	GCE	443:445	GCE	443:445	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	1	6	theme	carbon	425:430	arg1	electrode					432:440	a glassy carbon electrode	416:440	a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor	416:532	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	4	7	theme	LOQ	1075:1077	arg1	sensitivity					973:983	sensitivity	973:983	sensitivity (54.032 µA µM-1 cm-2)	973:1005	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	7	theme	LOQ	1075:1077	arg1	µM					1086:1087	LOQ; 0.545 µM	1075:1087	LOQ; 0.545 µM	1075:1087	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	1	8	theme	efficient	501:509	arg1	sensor					527:532	a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor	471:532	a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor	471:532	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	5	9	theme	various	1179:1185	arg1	samples					1201:1207	various environmental samples	1179:1207	various environmental samples	1179:1207	Additionally, various environmental samples were analyzed and obtained reliable analytical results.					
36354485	2	10	theme	composition	606:616	arg1	terms					577:581	terms	577:581	terms of morphology, surface composition, and optical properties	577:640	The synthesized NCs were characterized in terms of morphology, surface composition, and optical properties using FESEM, TEM, HRTEM, BET, XRD, XPS, FTIR, and UV-vis analysis.					
36354485	6	11	theme	developed	1331:1339	arg1	materials					1355:1363	newly developed nanocomposite materials	1325:1363	newly developed nanocomposite materials	1325:1363	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	1	12	theme	electrochemical	511:525	arg1	sensor					527:532	a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor	471:532	a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor	471:532	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	5	13	theme	environmental	1187:1199	arg1	samples					1201:1207	various environmental samples	1179:1207	various environmental samples	1179:1207	Additionally, various environmental samples were analyzed and obtained reliable analytical results.					
36354485	3	14	theme	detection	834:842	arg1	range					844:848	a wide detection range	827:848	a wide detection range (LDR) of 1.5~13.5 µM	827:869	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	3	14	theme	detection	834:842	arg1	LDR					851:853	LDR	851:853	LDR	851:853	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	2	15	theme	surface	598:604	arg1	composition					606:616	surface composition	598:616	surface composition	598:616	The synthesized NCs were characterized in terms of morphology, surface composition, and optical properties using FESEM, TEM, HRTEM, BET, XRD, XPS, FTIR, and UV-vis analysis.					
36354485	3	16	theme	Chitosan/SrSnO3	722:736	arg1	NC/GCE					738:743	Ag-decorated Chitosan/SrSnO3 NC/GCE	709:743	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS)	709:793	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	1	17	theme	sensor	527:532	arg1	development					456:466	the development	452:466	the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor	452:532	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	6	18	from	safety	1478:1483	arg1	scale					1535:1539	a broad scale	1527:1539	a broad scale	1527:1539	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	4	19	theme	µM-1	996:999	arg1	cm-2					1001:1004	54.032 µA µM-1 cm-2	986:1004	54.032 µA µM-1 cm-2	986:1004	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	19	theme	µM-1	996:999	arg1	sensitivity					973:983	sensitivity	973:983	sensitivity (54.032 µA µM-1 cm-2)	973:1005	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	1	20	used	used	370:373	arg2	they					347:350	they	347:350	they	347:350	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	1	20	used	used	370:373	arg2	substrates					395:404	electron-sensing substrates	378:404	electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor	378:532	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	4	21	theme	detection	1017:1025	arg1	cm-2					1001:1004	54.032 µA µM-1 cm-2	986:1004	54.032 µA µM-1 cm-2	986:1004	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	21	theme	detection	1017:1025	arg1	sensitivity					973:983	sensitivity	973:983	sensitivity (54.032 µA µM-1 cm-2)	973:1005	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	21	theme	detection	1017:1025	arg1	time					1120:1123	response time	1111:1123	response time	1111:1123	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	21	theme	detection	1017:1025	arg1	limit					1050:1054	limit	1050:1054	limit of quantification	1050:1072	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	21	theme	detection	1017:1025	arg1	limit					1008:1012	limit	1008:1012	limit of detection (LOD; 0.18 ± 0.01 µM)	1008:1047	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	21	theme	detection	1017:1025	arg1	µM					1086:1087	LOQ; 0.545 µM	1075:1087	LOQ; 0.545 µM	1075:1087	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	21	theme	detection	1017:1025	arg1	reproducibility					1090:1104	reproducibility	1090:1104	reproducibility	1090:1104	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	3	22	theme	DPV	919:921	arg1	approach					924:931	the differential pulse voltammetry (DPV) approach	883:931	the differential pulse voltammetry (DPV) approach	883:931	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	2	23	theme	morphology	586:595	arg1	terms					577:581	terms	577:581	terms of morphology, surface composition, and optical properties	577:640	The synthesized NCs were characterized in terms of morphology, surface composition, and optical properties using FESEM, TEM, HRTEM, BET, XRD, XPS, FTIR, and UV-vis analysis.					
36354485	4	24	theme	µA	993:994	arg1	cm-2					1001:1004	54.032 µA µM-1 cm-2	986:1004	54.032 µA µM-1 cm-2	986:1004	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	24	theme	µA	993:994	arg1	sensitivity					973:983	sensitivity	973:983	sensitivity (54.032 µA µM-1 cm-2)	973:1005	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	3	25	theme	wide	829:832	arg1	range					844:848	a wide detection range	827:848	a wide detection range (LDR) of 1.5~13.5 µM	827:869	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	3	25	theme	wide	829:832	arg1	LDR					851:853	LDR	851:853	LDR	851:853	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	3	26	theme	pulse	900:904	arg1	approach					924:931	the differential pulse voltammetry (DPV) approach	883:931	the differential pulse voltammetry (DPV) approach	883:931	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	3	27	theme	conducting	765:774	arg1	PEDOT					784:788	PEDOT	784:788	PEDOT:PSS	784:792	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	3	27	theme	conducting	765:774	arg1	binder					776:781	the conducting binder	761:781	the conducting binder (PEDOT:PSS)	761:793	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	6	28	with	probe	1314:1318	arg1	materials					1355:1363	newly developed nanocomposite materials	1325:1363	newly developed nanocomposite materials	1325:1363	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	3	29	theme	voltammetry	906:916	arg1	approach					924:931	the differential pulse voltammetry (DPV) approach	883:931	the differential pulse voltammetry (DPV) approach	883:931	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	0	30	theme	Efficient	0:8	arg1	Detection					10:18	Efficient Detection	0:18	Efficient Detection of 2,6-Dinitrophenol with Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites by Differential Pulse Voltammetry.	0:140	Efficient Detection of 2,6-Dinitrophenol with Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites by Differential Pulse Voltammetry.					
36354485	4	31	theme	excellent	1137:1145	arg1	results					1156:1162	excellent and good results	1137:1162	results	1156:1162	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	0	32	theme	2,6-Dinitrophenol	23:39	arg1	Detection					10:18	Efficient Detection	0:18	Efficient Detection of 2,6-Dinitrophenol with Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites by Differential Pulse Voltammetry.	0:140	Efficient Detection of 2,6-Dinitrophenol with Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites by Differential Pulse Voltammetry.					
36354485	2	33	theme	properties	631:640	arg1	terms					577:581	terms	577:581	terms of morphology, surface composition, and optical properties	577:640	The synthesized NCs were characterized in terms of morphology, surface composition, and optical properties using FESEM, TEM, HRTEM, BET, XRD, XPS, FTIR, and UV-vis analysis.					
36354485	2	34	theme	UV-vis	692:697	arg1	analysis					699:706	UV-vis analysis	692:706	UV-vis analysis	692:706	The synthesized NCs were characterized in terms of morphology, surface composition, and optical properties using FESEM, TEM, HRTEM, BET, XRD, XPS, FTIR, and UV-vis analysis.					
36354485	4	35	theme	good	1151:1154	arg1	results					1156:1162	excellent and good results	1137:1162	results	1156:1162	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	1	36	theme	silver	246:251	arg1	NCs					337:339	Ag-decorated Chitosan/SrSnO3 NCs	308:339	Ag-decorated Chitosan/SrSnO3 NCs	308:339	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	1	36	theme	silver	246:251	arg1	nanocomposites					292:305	silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites	246:305	silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs)	246:340	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	6	37	theme	nanocomposite	1341:1353	arg1	materials					1355:1363	newly developed nanocomposite materials	1325:1363	newly developed nanocomposite materials	1325:1363	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	4	38	dep	LOD	1028:1030	arg1	µM					1045:1046	0.18 ± 0.01 µM	1033:1046	LOD; 0.18 ± 0.01 µM	1028:1046	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	2	39	theme	optical	623:629	arg1	properties					631:640	optical properties	623:640	optical properties	623:640	The synthesized NCs were characterized in terms of morphology, surface composition, and optical properties using FESEM, TEM, HRTEM, BET, XRD, XPS, FTIR, and UV-vis analysis.					
36354485	5	40	theme	analytical	1245:1254	arg1	results					1256:1262	reliable analytical results	1236:1262	reliable analytical results	1236:1262	Additionally, various environmental samples were analyzed and obtained reliable analytical results.					
36354485	1	41	theme	nanoparticle-decorated	253:274	arg1	NCs					337:339	Ag-decorated Chitosan/SrSnO3 NCs	308:339	Ag-decorated Chitosan/SrSnO3 NCs	308:339	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	1	41	theme	nanoparticle-decorated	253:274	arg1	nanocomposites					292:305	silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites	246:305	silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs)	246:340	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	0	42	theme	Silver	46:51	arg1	Nanocomposites					92:105	Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites	46:105	Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites	46:105	Efficient Detection of 2,6-Dinitrophenol with Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites by Differential Pulse Voltammetry.					
36354485	4	43	theme	quantification	1059:1072	arg1	cm-2					1001:1004	54.032 µA µM-1 cm-2	986:1004	54.032 µA µM-1 cm-2	986:1004	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	43	theme	quantification	1059:1072	arg1	sensitivity					973:983	sensitivity	973:983	sensitivity (54.032 µA µM-1 cm-2)	973:1005	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	43	theme	quantification	1059:1072	arg1	time					1120:1123	response time	1111:1123	response time	1111:1123	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	43	theme	quantification	1059:1072	arg1	limit					1050:1054	limit	1050:1054	limit of quantification	1050:1072	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	43	theme	quantification	1059:1072	arg1	limit					1008:1012	limit	1008:1012	limit of detection (LOD; 0.18 ± 0.01 µM)	1008:1047	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	43	theme	quantification	1059:1072	arg1	µM					1086:1087	LOQ; 0.545 µM	1075:1087	LOQ; 0.545 µM	1075:1087	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	43	theme	quantification	1059:1072	arg1	reproducibility					1090:1104	reproducibility	1090:1104	reproducibility	1090:1104	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	6	44	theme	carcinogenic	1383:1394	arg1	contaminants					1396:1407	the carcinogenic contaminants	1379:1407	the carcinogenic contaminants from the environmental effluents	1379:1440	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	6	45	theme	electrochemical	1445:1459	arg1	approach					1461:1468	electrochemical approach	1445:1468	electrochemical approach for the safety of environmental and healthcare fields in a broad scale	1445:1539	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	1	46	theme	Chitosan/SrSnO3	276:290	arg1	NCs					337:339	Ag-decorated Chitosan/SrSnO3 NCs	308:339	Ag-decorated Chitosan/SrSnO3 NCs	308:339	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	1	46	theme	Chitosan/SrSnO3	276:290	arg1	nanocomposites					292:305	silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites	246:305	silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs)	246:340	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	1	47	theme	electron-sensing	378:393	arg1	substrates					395:404	electron-sensing substrates	378:404	electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor	378:532	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	1	47	theme	electron-sensing	378:393	arg1	they					347:350	they	347:350	they	347:350	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	0	48	with	2,6-Dinitrophenol	23:39	arg1	Nanocomposites					92:105	Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites	46:105	Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites	46:105	Efficient Detection of 2,6-Dinitrophenol with Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites by Differential Pulse Voltammetry.					
36354485	0	49	theme	Chitosan/SrSnO3	76:90	arg1	Nanocomposites					92:105	Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites	46:105	Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites	46:105	Efficient Detection of 2,6-Dinitrophenol with Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites by Differential Pulse Voltammetry.					
36354485	4	50	theme	sensor	946:951	arg1	sensitivity					973:983	sensitivity	973:983	sensitivity (54.032 µA µM-1 cm-2)	973:1005	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	50	theme	sensor	946:951	arg1	time					1120:1123	response time	1111:1123	response time	1111:1123	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	50	theme	sensor	946:951	arg1	limit					1050:1054	limit	1050:1054	limit of quantification	1050:1072	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	50	theme	sensor	946:951	arg1	limit					1008:1012	limit	1008:1012	limit of detection (LOD; 0.18 ± 0.01 µM)	1008:1047	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	50	theme	sensor	946:951	arg1	parameters					953:962	The 2,6-DNP sensor parameters	934:962	The 2,6-DNP sensor parameters	934:962	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	50	theme	sensor	946:951	arg1	reproducibility					1090:1104	reproducibility	1090:1104	reproducibility	1090:1104	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	3	51	from	2,6-DNP	816:822	arg1	range					844:848	a wide detection range	827:848	a wide detection range (LDR) of 1.5~13.5 µM	827:869	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	3	51	from	2,6-DNP	816:822	arg1	LDR					851:853	LDR	851:853	LDR	851:853	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	0	52	theme	Nanoparticle-Decorated	53:74	arg1	Nanocomposites					92:105	Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites	46:105	Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites	46:105	Efficient Detection of 2,6-Dinitrophenol with Silver Nanoparticle-Decorated Chitosan/SrSnO3 Nanocomposites by Differential Pulse Voltammetry.					
36354485	6	53	from	effluents	1432:1440	arg1	contaminants					1396:1407	the carcinogenic contaminants	1379:1407	the carcinogenic contaminants from the environmental effluents	1379:1440	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	1	54	theme	ultra-sonication	153:168	arg1	technique					170:178	an ultra-sonication technique	150:178	an ultra-sonication technique followed by a photoreduction technique	150:217	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	3	55	theme	Ag-decorated	709:720	arg1	NC/GCE					738:743	Ag-decorated Chitosan/SrSnO3 NC/GCE	709:743	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS)	709:793	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	4	56	theme	response	1111:1118	arg1	time					1120:1123	response time	1111:1123	response time	1111:1123	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	3	57	theme	µM	868:869	arg1	range					844:848	a wide detection range	827:848	a wide detection range (LDR) of 1.5~13.5 µM	827:869	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	3	57	theme	µM	868:869	arg1	LDR					851:853	LDR	851:853	LDR	851:853	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	6	58	theme	broad	1529:1533	arg1	scale					1535:1539	a broad scale	1527:1539	a broad scale	1527:1539	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	5	59	theme	reliable	1236:1243	arg1	results					1256:1262	reliable analytical results	1236:1262	reliable analytical results	1236:1262	Additionally, various environmental samples were analyzed and obtained reliable analytical results.					
36354485	6	60	theme	simplest	1281:1288	arg1	way					1290:1292	the simplest way	1277:1292	the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale	1277:1539	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	6	60	theme	simplest	1281:1288	arg1	it					1271:1272	it	1271:1272	it	1271:1272	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	1	61	theme	Ag-decorated	308:319	arg1	NCs					337:339	Ag-decorated Chitosan/SrSnO3 NCs	308:339	Ag-decorated Chitosan/SrSnO3 NCs	308:339	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	1	61	theme	Ag-decorated	308:319	arg1	nanocomposites					292:305	silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites	246:305	silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs)	246:340	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	6	62	theme	sensor	1307:1312	arg1	probe					1314:1318	a sensor probe	1305:1318	a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale	1305:1539	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	4	63	dep	detection	1017:1025	arg1	LOD					1028:1030	LOD	1028:1030	LOD; 0.18 ± 0.01 µM	1028:1046	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	6	64	theme	environmental	1418:1430	arg1	effluents					1432:1440	the environmental effluents	1414:1440	the environmental effluents	1414:1440	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	1	65	theme	Chitosan/SrSnO3	321:335	arg1	NCs					337:339	Ag-decorated Chitosan/SrSnO3 NCs	308:339	Ag-decorated Chitosan/SrSnO3 NCs	308:339	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	1	65	theme	Chitosan/SrSnO3	321:335	arg1	nanocomposites					292:305	silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites	246:305	silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs)	246:340	Herein, an ultra-sonication technique followed by a photoreduction technique was implemented to prepare silver nanoparticle-decorated Chitosan/SrSnO3 nanocomposites (Ag-decorated Chitosan/SrSnO3 NCs), and they were successively used as electron-sensing substrates coated on a glassy carbon electrode (GCE) for the development of a 2,6-dinitrophenol (2,6-DNP) efficient electrochemical sensor.					
36354485	2	66	theme	synthesized	539:549	arg1	NCs					551:553	The synthesized NCs	535:553	The synthesized NCs	535:553	The synthesized NCs were characterized in terms of morphology, surface composition, and optical properties using FESEM, TEM, HRTEM, BET, XRD, XPS, FTIR, and UV-vis analysis.					
36354485	3	67	dep	PEDOT	784:788	arg1	PSS					790:792	PSS	790:792	PEDOT:PSS	784:792	Ag-decorated Chitosan/SrSnO3 NC/GCE fabricated with the conducting binder (PEDOT:PSS) was found to analyze 2,6-DNP in a wide detection range (LDR) of 1.5~13.5 µM by applying the differential pulse voltammetry (DPV) approach.					
36354485	6	68	theme	fields	1517:1522	arg1	safety					1478:1483	the safety	1474:1483	the safety of environmental and healthcare fields in a broad scale	1474:1539	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	6	69	theme	environmental	1488:1500	arg1	fields					1517:1522	environmental and healthcare fields	1488:1522	environmental and healthcare fields	1488:1522	Thus, it is the simplest way to develop a sensor probe with newly developed nanocomposite materials for analyzing the carcinogenic contaminants from the environmental effluents by electrochemical approach for the safety of environmental and healthcare fields in a broad scale.					
36354485	4	70	theme	2,6-DNP	938:944	arg1	sensitivity					973:983	sensitivity	973:983	sensitivity (54.032 µA µM-1 cm-2)	973:1005	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	70	theme	2,6-DNP	938:944	arg1	time					1120:1123	response time	1111:1123	response time	1111:1123	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	70	theme	2,6-DNP	938:944	arg1	limit					1050:1054	limit	1050:1054	limit of quantification	1050:1072	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	70	theme	2,6-DNP	938:944	arg1	limit					1008:1012	limit	1008:1012	limit of detection (LOD; 0.18 ± 0.01 µM)	1008:1047	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	70	theme	2,6-DNP	938:944	arg1	parameters					953:962	The 2,6-DNP sensor parameters	934:962	The 2,6-DNP sensor parameters	934:962	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36354485	4	70	theme	2,6-DNP	938:944	arg1	reproducibility					1090:1104	reproducibility	1090:1104	reproducibility	1090:1104	The 2,6-DNP sensor parameters, such as sensitivity (54.032 µA µM-1 cm-2), limit of detection (LOD; 0.18 ± 0.01 µM), limit of quantification (LOQ; 0.545 µM) reproducibility, and response time, were found excellent and good results.					
36673516	12	0	theme	energy	2190:2195	arg1	kcal/mol					2170:2177	-6.20 kcal/mol	2164:2177	-6.20 kcal/mol of binding energy	2164:2195	The molecular docking prediction demonstrated that GalA had a good affinity toward AGE receptors by -6.20 kcal/mol of binding energy.					
36673516	13	1	theme	study	2211:2215	arg1	results					2217:2223	the study results	2207:2223	the study results	2207:2223	Overall, the study results provide a theoretical basis for broadening the application of CHP in the food industry.					
36673516	3	2	from	s	540:540	arg1	extract					481:487	extract	481:487	extract	481:487	Thus, the present study aimed to extract the pectin polysaccharide from Arabica coffee husk(s) (CHP).					
36673516	9	3	theme	characteristics	1338:1352	arg1	results					1354:1360	the structural characteristics results	1323:1360	the structural characteristics results	1323:1360	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	1	4	theme	coffee	194:199	arg1	husks					201:205	Arabica coffee husks	186:205	Arabica coffee husks	186:205	As primary coffee by-products, Arabica coffee husks are largely discarded during coffee-drying, posing a serious environmental threat.					
36673516	1	4	theme	coffee	194:199	arg1	by-products					173:183	primary coffee by-products	158:183	primary coffee by-products	158:183	As primary coffee by-products, Arabica coffee husks are largely discarded during coffee-drying, posing a serious environmental threat.					
36673516	5	5	dep	transform	763:771	arg1	infrared					773:780	infrared	773:780	transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM)	763:910	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	10	6	theme	elastic	1811:1817	arg1	G					1828:1828	G'	1828:1829	G'	1828:1829	The surface microstructure of CHP was rough with cracks, and its aqueous belonged to non-Newtonian fluid with a higher elastic modulus (G').					
36673516	10	6	theme	elastic	1811:1817	arg1	modulus					1819:1825	a higher elastic modulus	1802:1825	a higher elastic modulus (G')	1802:1830	The surface microstructure of CHP was rough with cracks, and its aqueous belonged to non-Newtonian fluid with a higher elastic modulus (G').					
36673516	5	7	theme	magnetic	844:851	arg1	resonance					853:861	proton nuclear magnetic resonance	829:861	proton nuclear magnetic resonance (1H NMR)	829:870	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	5	7	theme	magnetic	844:851	arg1	NMR					867:869	1H NMR	864:869	1H NMR	864:869	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	12	8	theme	good	2126:2129	arg1	affinity					2131:2138	a good affinity	2124:2138	a good affinity toward AGE receptors	2124:2159	The molecular docking prediction demonstrated that GalA had a good affinity toward AGE receptors by -6.20 kcal/mol of binding energy.					
36673516	0	9	theme	Coffee	141:146	arg1	Husks					148:152	Arabica Coffee Husks	133:152	Arabica Coffee Husks	133:152	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	9	10	theme	β-N-acetyl	1556:1565	arg1	glucosamine					1567:1577	β-N-acetyl glucosamine	1556:1577	β-N-acetyl glucosamine (GlcNAc, 5.17%)	1556:1593	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	10	theme	β-N-acetyl	1556:1565	arg1	rhamnose					1499:1506	rhamnose	1499:1506	rhamnose (Rha, 2.55%)	1499:1519	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	0	11	from	Activities	91:100	arg1	Husks					148:152	Arabica Coffee Husks	133:152	Arabica Coffee Husks	133:152	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	11	12	theme	inhibition	1975:1984	arg1	capacities					1986:1995	the inhibition capacities	1971:1995	the inhibition capacities of AGEs	1971:2003	Furthermore, the results of the antioxidant properties indicated that CHP possessed vigorous antioxidant activities in a dose manner, and the inhibition capacities of AGEs reached their highest of 66.0 ± 0.35% at 1.5 mg/mL of CHP.					
36673516	8	13	theme	CHP	1249:1251	arg1	yield					1253:1257	the CHP yield	1245:1257	the CHP yield	1245:1257	The results demonstrated that the CHP yield was 19.13 ± 0.85%, and its Mw was 1.04 × 106 Da.					
36673516	8	13	theme	CHP	1249:1251	arg1	%					1275:1275	19.13 ± 0.85%	1263:1275	19.13 ± 0.85%	1263:1275	The results demonstrated that the CHP yield was 19.13 ± 0.85%, and its Mw was 1.04 × 106 Da.					
36673516	10	14	theme	surface	1696:1702	arg1	rough					1730:1734	rough	1730:1734	rough	1730:1734	The surface microstructure of CHP was rough with cracks, and its aqueous belonged to non-Newtonian fluid with a higher elastic modulus (G').					
36673516	10	14	theme	surface	1696:1702	arg1	microstructure					1704:1717	The surface microstructure	1692:1717	The surface microstructure of CHP	1692:1724	The surface microstructure of CHP was rough with cracks, and its aqueous belonged to non-Newtonian fluid with a higher elastic modulus (G').					
36673516	10	15	with	fluid	1791:1795	arg1	G					1828:1828	G'	1828:1829	G'	1828:1829	The surface microstructure of CHP was rough with cracks, and its aqueous belonged to non-Newtonian fluid with a higher elastic modulus (G').					
36673516	10	15	with	fluid	1791:1795	arg1	modulus					1819:1825	a higher elastic modulus	1802:1825	a higher elastic modulus (G')	1802:1830	The surface microstructure of CHP was rough with cracks, and its aqueous belonged to non-Newtonian fluid with a higher elastic modulus (G').					
36673516	9	16	theme	pectic	1414:1419	arg1	polysaccharide					1421:1434	an amorphous and low-methoxyl pectic polysaccharide	1384:1434	an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond	1384:1473	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	16	theme	pectic	1414:1419	arg1	CHP					1376:1378	CHP	1376:1378	CHP	1376:1378	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	5	17	theme	scanning	877:884	arg1	SEM					907:909	SEM	907:909	SEM	907:909	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	5	17	theme	scanning	877:884	arg1	microscopy					895:904	scanning electron microscopy	877:904	scanning electron microscopy (SEM)	877:910	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	6	18	theme	CHP	973:975	arg1	properties					959:968	the rheological and antioxidant properties	927:968	the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations	927:1077	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	4	19	theme	permeation	665:674	arg1	chromatography					676:689	gel permeation chromatography	661:689	gel permeation chromatography (GPC)	661:695	The CHP yield was calculated after vacuum freeze-drying, and its average molecular weight (Mw) was detected by gel permeation chromatography (GPC).					
36673516	4	19	theme	permeation	665:674	arg1	GPC					692:694	GPC	692:694	GPC	692:694	The CHP yield was calculated after vacuum freeze-drying, and its average molecular weight (Mw) was detected by gel permeation chromatography (GPC).					
36673516	9	20	dep	glucosamine	1567:1577	arg1	GlcNAc					1580:1585	GlcNAc	1580:1585	GlcNAc	1580:1585	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	20	dep	glucosamine	1567:1577	arg1	%					1592:1592	5.17%	1588:1592	5.17%	1588:1592	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	5	21	theme	X-ray	804:808	arg1	XRD					823:825	XRD	823:825	XRD	823:825	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	5	21	theme	X-ray	804:808	arg1	diffraction					810:820	X-ray diffraction	804:820	X-ray diffraction (XRD)	804:826	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	1	22	theme	coffee	166:171	arg1	husks					201:205	Arabica coffee husks	186:205	Arabica coffee husks	186:205	As primary coffee by-products, Arabica coffee husks are largely discarded during coffee-drying, posing a serious environmental threat.					
36673516	1	22	theme	coffee	166:171	arg1	by-products					173:183	primary coffee by-products	158:183	primary coffee by-products	158:183	As primary coffee by-products, Arabica coffee husks are largely discarded during coffee-drying, posing a serious environmental threat.					
36673516	0	23	theme	Pectin	105:110	arg1	Polysaccharides					112:126	Pectin Polysaccharides	105:126	Pectin Polysaccharides from Arabica Coffee Husks	105:152	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	11	24	theme	antioxidant	1926:1936	arg1	activities					1938:1947	vigorous antioxidant activities	1917:1947	vigorous antioxidant activities	1917:1947	Furthermore, the results of the antioxidant properties indicated that CHP possessed vigorous antioxidant activities in a dose manner, and the inhibition capacities of AGEs reached their highest of 66.0 ± 0.35% at 1.5 mg/mL of CHP.					
36673516	2	25	theme	processing	425:434	arg1	properties					436:445	excellent processing properties	415:445	excellent processing properties	415:445	However, coffee husks could be used as potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties.					
36673516	5	26	theme	CHP	732:734	arg1	characteristics					713:727	The structural characteristics	698:727	The structural characteristics of CHP	698:734	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	6	27	theme	end	1029:1031	arg1	AGEs					1043:1046	AGEs	1043:1046	AGEs	1043:1046	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	6	27	theme	end	1029:1031	arg1	products					1033:1040	advanced glycation end products	1010:1040	advanced glycation end products (AGEs)	1010:1047	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	0	28	from	Properties	40:49	arg1	Husks					148:152	Arabica Coffee Husks	133:152	Arabica Coffee Husks	133:152	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	8	29	theme	1.04	1293:1296	arg1	×					1298:1298	×	1298:1298	×	1298:1298	The results demonstrated that the CHP yield was 19.13 ± 0.85%, and its Mw was 1.04 × 106 Da.					
36673516	0	30	theme	Structural	0:9	arg1	Characteristics					11:25	Structural Characteristics	0:25	Structural Characteristics	0:25	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	6	31	theme	advanced	1010:1017	arg1	AGEs					1043:1046	AGEs	1043:1046	AGEs	1043:1046	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	6	31	theme	advanced	1010:1017	arg1	products					1033:1040	advanced glycation end products	1010:1040	advanced glycation end products (AGEs)	1010:1047	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	11	32	theme	CHP	2059:2061	arg1	mg/mL					2050:2054	1.5 mg/mL	2046:2054	1.5 mg/mL of CHP	2046:2061	Furthermore, the results of the antioxidant properties indicated that CHP possessed vigorous antioxidant activities in a dose manner, and the inhibition capacities of AGEs reached their highest of 66.0 ± 0.35% at 1.5 mg/mL of CHP.					
36673516	8	33	theme	106	1300:1302	arg1	×					1298:1298	×	1298:1298	×	1298:1298	The results demonstrated that the CHP yield was 19.13 ± 0.85%, and its Mw was 1.04 × 106 Da.					
36673516	7	34	theme	galacturonic	1130:1141	arg1	GalA					1149:1152	GalA	1149:1152	GalA	1149:1152	The interaction mechanisms between galacturonic acid (GalA) and the AGE receptor were analyzed using molecular docking.					
36673516	7	34	theme	galacturonic	1130:1141	arg1	acid					1143:1146	galacturonic acid	1130:1146	galacturonic acid (GalA)	1130:1153	The interaction mechanisms between galacturonic acid (GalA) and the AGE receptor were analyzed using molecular docking.					
36673516	6	35	theme	capacities	996:1005	arg1	properties					959:968	the rheological and antioxidant properties	927:968	the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations	927:1077	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	11	36	theme	properties	1877:1886	arg1	results					1850:1856	the results	1846:1856	the results of the antioxidant properties	1846:1886	Furthermore, the results of the antioxidant properties indicated that CHP possessed vigorous antioxidant activities in a dose manner, and the inhibition capacities of AGEs reached their highest of 66.0 ± 0.35% at 1.5 mg/mL of CHP.					
36673516	9	37	theme	results	1354:1360	arg1	results					1312:1318	The results	1308:1318	The results of the structural characteristics results	1308:1360	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	13	38	from	application	2272:2282	arg1	industry					2303:2310	the food industry	2294:2310	the food industry	2294:2310	Overall, the study results provide a theoretical basis for broadening the application of CHP in the food industry.					
36673516	12	39	theme	molecular	2068:2076	arg1	prediction					2086:2095	The molecular docking prediction	2064:2095	The molecular docking prediction	2064:2095	The molecular docking prediction demonstrated that GalA had a good affinity toward AGE receptors by -6.20 kcal/mol of binding energy.					
36673516	13	40	theme	food	2298:2301	arg1	industry					2303:2310	the food industry	2294:2310	the food industry	2294:2310	Overall, the study results provide a theoretical basis for broadening the application of CHP in the food industry.					
36673516	3	41	theme	Arabica	520:526	arg1	s					540:540	Arabica coffee husk(s)	520:541	Arabica coffee husk(s) (CHP)	520:547	Thus, the present study aimed to extract the pectin polysaccharide from Arabica coffee husk(s) (CHP).					
36673516	3	41	theme	Arabica	520:526	arg1	CHP					544:546	CHP	544:546	CHP	544:546	Thus, the present study aimed to extract the pectin polysaccharide from Arabica coffee husk(s) (CHP).					
36673516	13	42	theme	theoretical	2235:2245	arg1	basis					2247:2251	a theoretical basis	2233:2251	a theoretical basis for broadening the application of CHP in the food industry	2233:2310	Overall, the study results provide a theoretical basis for broadening the application of CHP in the food industry.					
36673516	10	43	theme	non-Newtonian	1777:1789	arg1	fluid					1791:1795	non-Newtonian fluid	1777:1795	non-Newtonian fluid with a higher elastic modulus (G')	1777:1830	The surface microstructure of CHP was rough with cracks, and its aqueous belonged to non-Newtonian fluid with a higher elastic modulus (G').					
36673516	8	44	theme	±	1269:1269	arg1	yield					1253:1257	the CHP yield	1245:1257	the CHP yield	1245:1257	The results demonstrated that the CHP yield was 19.13 ± 0.85%, and its Mw was 1.04 × 106 Da.					
36673516	8	44	theme	±	1269:1269	arg1	%					1275:1275	19.13 ± 0.85%	1263:1275	19.13 ± 0.85%	1263:1275	The results demonstrated that the CHP yield was 19.13 ± 0.85%, and its Mw was 1.04 × 106 Da.					
36673516	4	45	theme	gel	661:663	arg1	chromatography					676:689	gel permeation chromatography	661:689	gel permeation chromatography (GPC)	661:695	The CHP yield was calculated after vacuum freeze-drying, and its average molecular weight (Mw) was detected by gel permeation chromatography (GPC).					
36673516	4	45	theme	gel	661:663	arg1	GPC					692:694	GPC	692:694	GPC	692:694	The CHP yield was calculated after vacuum freeze-drying, and its average molecular weight (Mw) was detected by gel permeation chromatography (GPC).					
36673516	11	46	theme	AGEs	2000:2003	arg1	capacities					1986:1995	the inhibition capacities	1971:1995	the inhibition capacities of AGEs	1971:2003	Furthermore, the results of the antioxidant properties indicated that CHP possessed vigorous antioxidant activities in a dose manner, and the inhibition capacities of AGEs reached their highest of 66.0 ± 0.35% at 1.5 mg/mL of CHP.					
36673516	2	47	theme	high	392:395	arg1	bioactivities					397:409	high bioactivities	392:409	high bioactivities	392:409	However, coffee husks could be used as potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties.					
36673516	5	48	theme	proton	829:834	arg1	resonance					853:861	proton nuclear magnetic resonance	829:861	proton nuclear magnetic resonance (1H NMR)	829:870	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	5	48	theme	proton	829:834	arg1	NMR					867:869	1H NMR	864:869	1H NMR	864:869	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	10	49	theme	higher	1804:1809	arg1	G					1828:1828	G'	1828:1829	G'	1828:1829	The surface microstructure of CHP was rough with cracks, and its aqueous belonged to non-Newtonian fluid with a higher elastic modulus (G').					
36673516	10	49	theme	higher	1804:1809	arg1	modulus					1819:1825	a higher elastic modulus	1802:1825	a higher elastic modulus (G')	1802:1830	The surface microstructure of CHP was rough with cracks, and its aqueous belonged to non-Newtonian fluid with a higher elastic modulus (G').					
36673516	3	50	theme	husk	535:538	arg1	s					540:540	Arabica coffee husk(s)	520:541	Arabica coffee husk(s) (CHP)	520:547	Thus, the present study aimed to extract the pectin polysaccharide from Arabica coffee husk(s) (CHP).					
36673516	3	50	theme	husk	535:538	arg1	CHP					544:546	CHP	544:546	CHP	544:546	Thus, the present study aimed to extract the pectin polysaccharide from Arabica coffee husk(s) (CHP).					
36673516	1	51	theme	environmental	268:280	arg1	threat					282:287	a serious environmental threat	258:287	a serious environmental threat	258:287	As primary coffee by-products, Arabica coffee husks are largely discarded during coffee-drying, posing a serious environmental threat.					
36673516	9	52	theme	galacturonic	1522:1533	arg1	acid					1535:1538	galacturonic acid	1522:1538	galacturonic acid (GalA, 45.01%)	1522:1553	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	52	theme	galacturonic	1522:1533	arg1	rhamnose					1499:1506	rhamnose	1499:1506	rhamnose (Rha, 2.55%)	1499:1519	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	7	53	theme	molecular	1196:1204	arg1	docking					1206:1212	molecular docking	1196:1212	molecular docking	1196:1212	The interaction mechanisms between galacturonic acid (GalA) and the AGE receptor were analyzed using molecular docking.					
36673516	9	54	theme	structural	1327:1336	arg1	characteristics					1338:1352	the structural characteristics	1323:1352	the structural characteristics results	1323:1360	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	55	theme	α-	1451:1452	arg1	bond					1470:1473	an α-(1→6) glycosidic bond	1448:1473	an α-(1→6) glycosidic bond	1448:1473	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	4	56	theme	vacuum	585:590	arg1	freeze-drying					592:604	vacuum freeze-drying	585:604	vacuum freeze-drying	585:604	The CHP yield was calculated after vacuum freeze-drying, and its average molecular weight (Mw) was detected by gel permeation chromatography (GPC).					
36673516	2	57	theme	pectin	363:368	arg1	polysaccharides					370:384	pectin polysaccharides	363:384	pectin polysaccharides	363:384	However, coffee husks could be used as potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties.					
36673516	2	58	theme	coffee	299:304	arg1	husks					306:310	coffee husks	299:310	coffee husks	299:310	However, coffee husks could be used as potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties.					
36673516	2	58	theme	coffee	299:304	arg1	material					339:346	potential material	329:346	potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties	329:445	However, coffee husks could be used as potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties.					
36673516	1	59	theme	Arabica	186:192	arg1	husks					201:205	Arabica coffee husks	186:205	Arabica coffee husks	186:205	As primary coffee by-products, Arabica coffee husks are largely discarded during coffee-drying, posing a serious environmental threat.					
36673516	1	59	theme	Arabica	186:192	arg1	by-products					173:183	primary coffee by-products	158:183	primary coffee by-products	158:183	As primary coffee by-products, Arabica coffee husks are largely discarded during coffee-drying, posing a serious environmental threat.					
36673516	10	60	with	rough	1730:1734	arg1	cracks					1741:1746	cracks	1741:1746	cracks	1741:1746	The surface microstructure of CHP was rough with cracks, and its aqueous belonged to non-Newtonian fluid with a higher elastic modulus (G').					
36673516	11	61	theme	dose	1954:1957	arg1	manner					1959:1964	a dose manner	1952:1964	a dose manner	1952:1964	Furthermore, the results of the antioxidant properties indicated that CHP possessed vigorous antioxidant activities in a dose manner, and the inhibition capacities of AGEs reached their highest of 66.0 ± 0.35% at 1.5 mg/mL of CHP.					
36673516	9	62	dep	Rha	1509:1511	arg1	%					1518:1518	2.55%	1514:1518	2.55%	1514:1518	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	2	63	theme	potential	329:337	arg1	husks					306:310	coffee husks	299:310	coffee husks	299:310	However, coffee husks could be used as potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties.					
36673516	2	63	theme	potential	329:337	arg1	material					339:346	potential material	329:346	potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties	329:445	However, coffee husks could be used as potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties.					
36673516	5	64	theme	nuclear	836:842	arg1	resonance					853:861	proton nuclear magnetic resonance	829:861	proton nuclear magnetic resonance (1H NMR)	829:870	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	5	64	theme	nuclear	836:842	arg1	NMR					867:869	1H NMR	864:869	1H NMR	864:869	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	5	65	dep	Fourier	755:761	arg1	transform					763:771	transform	763:771	transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM)	763:910	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	9	66	theme	Ara	1679:1681	arg1	glucose					1596:1602	glucose	1596:1602	glucose (Glc, 32.29%)	1596:1616	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	66	theme	Ara	1679:1681	arg1	%					1688:1688	Ara, 7.42%	1679:1688	%	1688:1688	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	4	67	theme	molecular	623:631	arg1	Mw					641:642	Mw	641:642	Mw	641:642	The CHP yield was calculated after vacuum freeze-drying, and its average molecular weight (Mw) was detected by gel permeation chromatography (GPC).					
36673516	4	67	theme	molecular	623:631	arg1	weight					633:638	its average molecular weight	611:638	its average molecular weight (Mw)	611:643	The CHP yield was calculated after vacuum freeze-drying, and its average molecular weight (Mw) was detected by gel permeation chromatography (GPC).					
36673516	9	68	theme	amorphous	1387:1395	arg1	polysaccharide					1421:1434	an amorphous and low-methoxyl pectic polysaccharide	1384:1434	an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond	1384:1473	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	68	theme	amorphous	1387:1395	arg1	CHP					1376:1378	CHP	1376:1378	CHP	1376:1378	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	5	69	theme	structural	702:711	arg1	characteristics					713:727	The structural characteristics	698:727	The structural characteristics of CHP	698:734	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	9	70	theme	low-methoxyl	1401:1412	arg1	polysaccharide					1421:1434	an amorphous and low-methoxyl pectic polysaccharide	1384:1434	an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond	1384:1473	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	70	theme	low-methoxyl	1401:1412	arg1	CHP					1376:1378	CHP	1376:1378	CHP	1376:1378	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	6	71	with	CHP	973:975	arg1	concentrations					1064:1077	different concentrations	1054:1077	different concentrations	1054:1077	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	12	72	contain	had	2120:2122	arg1	GalA					2115:2118	GalA	2115:2118	GalA	2115:2118	The molecular docking prediction demonstrated that GalA had a good affinity toward AGE receptors by -6.20 kcal/mol of binding energy.					
36673516	12	72	contain	had	2120:2122	arg2	affinity					2131:2138	a good affinity	2124:2138	a good affinity toward AGE receptors	2124:2159	The molecular docking prediction demonstrated that GalA had a good affinity toward AGE receptors by -6.20 kcal/mol of binding energy.					
36673516	0	73	theme	Anti-Glycosylation	72:89	arg1	Activities					91:100	Antioxidant and Anti-Glycosylation Activities	56:100	Antioxidant and Anti-Glycosylation Activities	56:100	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	1	74	theme	primary	158:164	arg1	husks					201:205	Arabica coffee husks	186:205	Arabica coffee husks	186:205	As primary coffee by-products, Arabica coffee husks are largely discarded during coffee-drying, posing a serious environmental threat.					
36673516	1	74	theme	primary	158:164	arg1	by-products					173:183	primary coffee by-products	158:183	primary coffee by-products	158:183	As primary coffee by-products, Arabica coffee husks are largely discarded during coffee-drying, posing a serious environmental threat.					
36673516	11	75	theme	vigorous	1917:1924	arg1	activities					1938:1947	vigorous antioxidant activities	1917:1947	vigorous antioxidant activities	1917:1947	Furthermore, the results of the antioxidant properties indicated that CHP possessed vigorous antioxidant activities in a dose manner, and the inhibition capacities of AGEs reached their highest of 66.0 ± 0.35% at 1.5 mg/mL of CHP.					
36673516	13	76	theme	CHP	2287:2289	arg1	application					2272:2282	the application	2268:2282	the application of CHP in the food industry	2268:2310	Overall, the study results provide a theoretical basis for broadening the application of CHP in the food industry.					
36673516	8	77	theme	19.13	1263:1267	arg1	yield					1253:1257	the CHP yield	1245:1257	the CHP yield	1245:1257	The results demonstrated that the CHP yield was 19.13 ± 0.85%, and its Mw was 1.04 × 106 Da.					
36673516	8	77	theme	19.13	1263:1267	arg1	%					1275:1275	19.13 ± 0.85%	1263:1275	19.13 ± 0.85%	1263:1275	The results demonstrated that the CHP yield was 19.13 ± 0.85%, and its Mw was 1.04 × 106 Da.					
36673516	10	78	theme	CHP	1722:1724	arg1	rough					1730:1734	rough	1730:1734	rough	1730:1734	The surface microstructure of CHP was rough with cracks, and its aqueous belonged to non-Newtonian fluid with a higher elastic modulus (G').					
36673516	10	78	theme	CHP	1722:1724	arg1	microstructure					1704:1717	The surface microstructure	1692:1717	The surface microstructure of CHP	1692:1724	The surface microstructure of CHP was rough with cracks, and its aqueous belonged to non-Newtonian fluid with a higher elastic modulus (G').					
36673516	7	79	theme	AGE	1163:1165	arg1	receptor					1167:1174	the AGE receptor	1159:1174	the AGE receptor	1159:1174	The interaction mechanisms between galacturonic acid (GalA) and the AGE receptor were analyzed using molecular docking.					
36673516	5	80	theme	1H	864:865	arg1	resonance					853:861	proton nuclear magnetic resonance	829:861	proton nuclear magnetic resonance (1H NMR)	829:870	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	5	80	theme	1H	864:865	arg1	NMR					867:869	1H NMR	864:869	1H NMR	864:869	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	6	81	theme	antioxidant	947:957	arg1	properties					959:968	the rheological and antioxidant properties	927:968	the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations	927:1077	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	9	82	dep	glucose	1596:1602	arg1	Glc					1605:1607	Glc	1605:1607	Glc	1605:1607	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	82	dep	glucose	1596:1602	arg1	%					1615:1615	32.29%	1610:1615	32.29%	1610:1615	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	0	83	from	Characteristics	11:25	arg1	Husks					148:152	Arabica Coffee Husks	133:152	Arabica Coffee Husks	133:152	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	0	84	theme	Polysaccharides	112:126	arg1	Activities					91:100	Antioxidant and Anti-Glycosylation Activities	56:100	Antioxidant and Anti-Glycosylation Activities	56:100	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	0	84	theme	Polysaccharides	112:126	arg1	Characteristics					11:25	Structural Characteristics	0:25	Structural Characteristics	0:25	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	0	84	theme	Polysaccharides	112:126	arg1	Properties					40:49	Rheological Properties	28:49	Rheological Properties	28:49	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	9	85	dep	xylose	1643:1648	arg1	Xyl					1651:1653	Xyl	1651:1653	Xyl	1651:1653	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	85	dep	xylose	1643:1648	arg1	%					1660:1660	0.76%	1656:1660	0.76%	1656:1660	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	86	theme	1→6	1454:1456	arg1	bond					1470:1473	an α-(1→6) glycosidic bond	1448:1473	an α-(1→6) glycosidic bond	1448:1473	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	6	87	theme	rheological	931:941	arg1	properties					959:968	the rheological and antioxidant properties	927:968	the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations	927:1077	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	12	88	theme	AGE	2147:2149	arg1	receptors					2151:2159	AGE receptors	2147:2159	AGE receptors	2147:2159	The molecular docking prediction demonstrated that GalA had a good affinity toward AGE receptors by -6.20 kcal/mol of binding energy.					
36673516	6	89	theme	different	1054:1062	arg1	concentrations					1064:1077	different concentrations	1054:1077	different concentrations	1054:1077	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	0	90	theme	Arabica	133:139	arg1	Husks					148:152	Arabica Coffee Husks	133:152	Arabica Coffee Husks	133:152	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	9	91	theme	glycosidic	1459:1468	arg1	bond					1470:1473	an α-(1→6) glycosidic bond	1448:1473	an α-(1→6) glycosidic bond	1448:1473	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	6	92	theme	products	1033:1040	arg1	capacities					996:1005	the inhibition capacities	981:1005	the inhibition capacities of advanced glycation end products (AGEs) with different concentrations	981:1077	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	6	92	theme	products	1033:1040	arg1	CHP					973:975	CHP	973:975	CHP	973:975	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	2	93	theme	excellent	415:423	arg1	properties					436:445	excellent processing properties	415:445	excellent processing properties	415:445	However, coffee husks could be used as potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties.					
36673516	9	94	dep	acid	1535:1538	arg1	GalA					1541:1544	GalA	1541:1544	GalA	1541:1544	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	94	dep	acid	1535:1538	arg1	%					1552:1552	45.01%	1547:1552	45.01%	1547:1552	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	5	95	theme	electron	886:893	arg1	SEM					907:909	SEM	907:909	SEM	907:909	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	5	95	theme	electron	886:893	arg1	microscopy					895:904	scanning electron microscopy	877:904	scanning electron microscopy (SEM)	877:910	The structural characteristics of CHP were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM).					
36673516	6	96	theme	glycation	1019:1027	arg1	AGEs					1043:1046	AGEs	1043:1046	AGEs	1043:1046	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	6	96	theme	glycation	1019:1027	arg1	products					1033:1040	advanced glycation end products	1010:1040	advanced glycation end products (AGEs)	1010:1047	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	4	97	theme	CHP	554:556	arg1	yield					558:562	The CHP yield	550:562	The CHP yield	550:562	The CHP yield was calculated after vacuum freeze-drying, and its average molecular weight (Mw) was detected by gel permeation chromatography (GPC).					
36673516	3	98	theme	present	458:464	arg1	study					466:470	the present study	454:470	the present study	454:470	Thus, the present study aimed to extract the pectin polysaccharide from Arabica coffee husk(s) (CHP).					
36673516	0	99	theme	Rheological	28:38	arg1	Properties					40:49	Rheological Properties	28:49	Rheological Properties	28:49	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	11	100	contain	possessed	1907:1915	arg2	activities					1938:1947	vigorous antioxidant activities	1917:1947	vigorous antioxidant activities	1917:1947	Furthermore, the results of the antioxidant properties indicated that CHP possessed vigorous antioxidant activities in a dose manner, and the inhibition capacities of AGEs reached their highest of 66.0 ± 0.35% at 1.5 mg/mL of CHP.					
36673516	11	100	contain	possessed	1907:1915	arg1	CHP					1903:1905	CHP	1903:1905	CHP	1903:1905	Furthermore, the results of the antioxidant properties indicated that CHP possessed vigorous antioxidant activities in a dose manner, and the inhibition capacities of AGEs reached their highest of 66.0 ± 0.35% at 1.5 mg/mL of CHP.					
36673516	3	101	theme	pectin	493:498	arg1	polysaccharide					500:513	the pectin polysaccharide	489:513	the pectin polysaccharide	489:513	Thus, the present study aimed to extract the pectin polysaccharide from Arabica coffee husk(s) (CHP).					
36673516	7	102	theme	interaction	1099:1109	arg1	mechanisms					1111:1120	The interaction mechanisms	1095:1120	The interaction mechanisms between galacturonic acid (GalA) and the AGE receptor	1095:1174	The interaction mechanisms between galacturonic acid (GalA) and the AGE receptor were analyzed using molecular docking.					
36673516	6	103	theme	inhibition	985:994	arg1	capacities					996:1005	the inhibition capacities	981:1005	the inhibition capacities of advanced glycation end products (AGEs) with different concentrations	981:1077	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	9	104	theme	Gal	1630:1632	arg1	%					1639:1639	Gal, 6.80%	1630:1639	%	1639:1639	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	9	104	theme	Gal	1630:1632	arg1	galactose					1619:1627	galactose	1619:1627	galactose (Gal, 6.80%)	1619:1640	The results of the structural characteristics results revealed that CHP was an amorphous and low-methoxyl pectic polysaccharide linked with an α-(1→6) glycosidic bond, and mainly composed of rhamnose (Rha, 2.55%), galacturonic acid (GalA, 45.01%), β-N-acetyl glucosamine (GlcNAc, 5.17%), glucose (Glc, 32.29%), galactose (Gal, 6.80%), xylose (Xyl, 0.76%), and arabinose (Ara, 7.42%).					
36673516	12	105	theme	docking	2078:2084	arg1	prediction					2086:2095	The molecular docking prediction	2064:2095	The molecular docking prediction	2064:2095	The molecular docking prediction demonstrated that GalA had a good affinity toward AGE receptors by -6.20 kcal/mol of binding energy.					
36673516	0	106	from	Husks	148:152	arg1	Activities					91:100	Antioxidant and Anti-Glycosylation Activities	56:100	Antioxidant and Anti-Glycosylation Activities	56:100	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	0	106	from	Husks	148:152	arg1	Polysaccharides					112:126	Pectin Polysaccharides	105:126	Pectin Polysaccharides from Arabica Coffee Husks	105:152	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	0	106	from	Husks	148:152	arg1	Characteristics					11:25	Structural Characteristics	0:25	Structural Characteristics	0:25	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	0	106	from	Husks	148:152	arg1	Properties					40:49	Rheological Properties	28:49	Rheological Properties	28:49	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	0	107	theme	Antioxidant	56:66	arg1	Activities					91:100	Antioxidant and Anti-Glycosylation Activities	56:100	Antioxidant and Anti-Glycosylation Activities	56:100	Structural Characteristics, Rheological Properties, and Antioxidant and Anti-Glycosylation Activities of Pectin Polysaccharides from Arabica Coffee Husks.					
36673516	11	108	theme	antioxidant	1865:1875	arg1	properties					1877:1886	the antioxidant properties	1861:1886	the antioxidant properties	1861:1886	Furthermore, the results of the antioxidant properties indicated that CHP possessed vigorous antioxidant activities in a dose manner, and the inhibition capacities of AGEs reached their highest of 66.0 ± 0.35% at 1.5 mg/mL of CHP.					
36673516	3	109	theme	coffee	528:533	arg1	s					540:540	Arabica coffee husk(s)	520:541	Arabica coffee husk(s) (CHP)	520:547	Thus, the present study aimed to extract the pectin polysaccharide from Arabica coffee husk(s) (CHP).					
36673516	3	109	theme	coffee	528:533	arg1	CHP					544:546	CHP	544:546	CHP	544:546	Thus, the present study aimed to extract the pectin polysaccharide from Arabica coffee husk(s) (CHP).					
36673516	2	110	with	polysaccharides	370:384	arg1	bioactivities					397:409	high bioactivities	392:409	high bioactivities	392:409	However, coffee husks could be used as potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties.					
36673516	2	110	with	polysaccharides	370:384	arg1	properties					436:445	excellent processing properties	415:445	excellent processing properties	415:445	However, coffee husks could be used as potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties.					
36673516	2	111	used	used	321:324	arg2	material					339:346	potential material	329:346	potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties	329:445	However, coffee husks could be used as potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties.					
36673516	2	111	used	used	321:324	arg2	husks					306:310	coffee husks	299:310	coffee husks	299:310	However, coffee husks could be used as potential material for extracting pectin polysaccharides, with high bioactivities and excellent processing properties.					
36673516	6	112	with	capacities	996:1005	arg1	concentrations					1064:1077	different concentrations	1054:1077	different concentrations	1054:1077	Additionally, the rheological and antioxidant properties of CHP and the inhibition capacities of advanced glycation end products (AGEs) with different concentrations were evaluated.					
36673516	12	113	theme	binding	2182:2188	arg1	energy					2190:2195	binding energy	2182:2195	binding energy	2182:2195	The molecular docking prediction demonstrated that GalA had a good affinity toward AGE receptors by -6.20 kcal/mol of binding energy.					
36673516	4	114	theme	average	615:621	arg1	Mw					641:642	Mw	641:642	Mw	641:642	The CHP yield was calculated after vacuum freeze-drying, and its average molecular weight (Mw) was detected by gel permeation chromatography (GPC).					
36673516	4	114	theme	average	615:621	arg1	weight					633:638	its average molecular weight	611:638	its average molecular weight (Mw)	611:643	The CHP yield was calculated after vacuum freeze-drying, and its average molecular weight (Mw) was detected by gel permeation chromatography (GPC).					
36673516	1	115	theme	serious	260:266	arg1	threat					282:287	a serious environmental threat	258:287	a serious environmental threat	258:287	As primary coffee by-products, Arabica coffee husks are largely discarded during coffee-drying, posing a serious environmental threat.					
35840399	9	0	used	used	1420:1423	arg2	scores					1403:1408	PC scores	1400:1408	PC scores	1400:1408	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	9	0	used	used	1420:1423	arg2	covariates					1428:1437	covariates	1428:1437	covariates	1428:1437	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	6	1	theme	milk	1003:1006	arg1	traits					1020:1025	milk composition traits	1003:1025	milk composition traits	1003:1025	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	10	2	theme	present	1544:1550	arg1	work					1552:1555	the present work	1540:1555	the present work	1540:1555	Results of the present work demonstrated that the probability of having NC samples increases as milk contents of proteins and chlorides and somatic cell score increase.					
35840399	4	3	theme	genetic	806:812	arg1	background					814:823	the genetic background	802:823	the genetic background of milk coagulation ability	802:851	We also investigated the genetic background of milk coagulation ability.					
35840399	6	4	theme	firming	1091:1097	arg1	time					1099:1102	curd firming time	1086:1102	curd firming time	1086:1102	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	2	5	theme	impaired	269:276	arg1	properties					290:299	impaired coagulation properties	269:299	impaired coagulation properties	269:299	The occurrence of milk with impaired coagulation properties is an obstacle to cheese processing and, in turn, to the profitability of the dairy companies.					
35840399	5	6	from	flocks	926:931	arg1	farmed					913:918	farmed	913:918	farmed	913:918	Individual milk samples were collected from 996 Sarda ewes farmed in 47 flocks located in Sardinia (Italy).					
35840399	2	7	theme	milk	259:262	arg1	occurrence					245:254	The occurrence	241:254	The occurrence of milk with impaired coagulation properties	241:299	The occurrence of milk with impaired coagulation properties is an obstacle to cheese processing and, in turn, to the profitability of the dairy companies.					
35840399	2	7	theme	milk	259:262	arg1	obstacle					307:314	an obstacle	304:314	an obstacle to cheese processing and, in turn, to the profitability of the dairy companies	304:393	The occurrence of milk with impaired coagulation properties is an obstacle to cheese processing and, in turn, to the profitability of the dairy companies.					
35840399	13	8	theme	coagulation	2167:2177	arg1	status					2179:2184	milk coagulation status	2162:2184	milk coagulation status	2162:2184	Genetic analysis of milk coagulation status and of the extracted PC confirmed the genetic background of the milk coagulability of sheep milk.					
35840399	9	9	theme	coagulation	1509:1519	arg1	status					1521:1526	milk coagulation status	1504:1526	milk coagulation status	1504:1526	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	6	10	theme	coagulation	1068:1078	arg1	time					1080:1083	rennet coagulation time	1061:1083	rennet coagulation time	1061:1083	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	6	11	theme	MIR	1129:1131	arg1	spectra					1133:1139	MIR spectra	1129:1139	MIR spectra	1129:1139	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	3	12	theme	properties	530:539	arg1	effect					499:504	the effect	495:504	the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status	495:778	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	11	13	theme	composition	1912:1922	arg1	traits					1924:1929	routinely collected milk composition traits	1887:1929	routinely collected milk composition traits	1887:1929	The analysis of PC extracted from milk spectra that influenced coagulation status highlighted key regions associated with lactose and protein concentrations, and others not associated with routinely collected milk composition traits.					
35840399	11	14	theme	key	1792:1794	arg1	regions					1796:1802	key regions	1792:1802	key regions associated with lactose and protein concentrations	1792:1853	The analysis of PC extracted from milk spectra that influenced coagulation status highlighted key regions associated with lactose and protein concentrations, and others not associated with routinely collected milk composition traits.					
35840399	3	15	theme	milk	509:512	arg1	properties					530:539	milk physicochemical properties	509:539	milk physicochemical properties	509:539	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	1	16	theme	sheep	195:199	arg1	milk					201:204	almost all sheep milk	184:204	almost all sheep milk	184:204	Milk coagulation ability is of central importance for the sheep dairy industry because almost all sheep milk is destined for cheese processing.					
35840399	11	17	theme	collected	1897:1905	arg1	traits					1924:1929	routinely collected milk composition traits	1887:1929	routinely collected milk composition traits	1887:1929	The analysis of PC extracted from milk spectra that influenced coagulation status highlighted key regions associated with lactose and protein concentrations, and others not associated with routinely collected milk composition traits.					
35840399	8	18	theme	coagulation	1299:1309	arg1	status					1311:1316	milk coagulation status	1294:1316	milk coagulation status	1294:1316	A logistic regression approach was used to test the effect of milk-related traits on milk coagulation status.					
35840399	11	19	theme	PC	1714:1715	arg1	analysis					1702:1709	The analysis	1698:1709	The analysis of PC	1698:1715	The analysis of PC extracted from milk spectra that influenced coagulation status highlighted key regions associated with lactose and protein concentrations, and others not associated with routinely collected milk composition traits.					
35840399	3	20	theme	mid-infrared	690:701	arg1	spectroscopy					703:714	mid-infrared spectroscopy	690:714	mid-infrared spectroscopy (MIR)	690:720	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	3	20	theme	mid-infrared	690:701	arg1	MIR					717:719	MIR	717:719	MIR	717:719	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	10	21	theme	milk	1625:1628	arg1	contents					1630:1637	milk contents	1625:1637	milk contents of proteins and chlorides	1625:1663	Results of the present work demonstrated that the probability of having NC samples increases as milk contents of proteins and chlorides and somatic cell score increase.					
35840399	2	22	with	occurrence	245:254	arg1	properties					290:299	impaired coagulation properties	269:299	impaired coagulation properties	269:299	The occurrence of milk with impaired coagulation properties is an obstacle to cheese processing and, in turn, to the profitability of the dairy companies.					
35840399	4	23	theme	coagulation	833:843	arg1	ability					845:851	milk coagulation ability	828:851	milk coagulation ability	828:851	We also investigated the genetic background of milk coagulation ability.					
35840399	10	24	theme	somatic	1669:1675	arg1	score					1682:1686	somatic cell score	1669:1686	somatic cell score	1669:1686	Results of the present work demonstrated that the probability of having NC samples increases as milk contents of proteins and chlorides and somatic cell score increase.					
35840399	6	25	theme	curd	1109:1112	arg1	firmness					1114:1121	curd firmness	1109:1121	curd firmness	1109:1121	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	13	26	theme	milk	2250:2253	arg1	coagulability					2255:2267	the milk coagulability	2246:2267	the milk coagulability of sheep milk	2246:2281	Genetic analysis of milk coagulation status and of the extracted PC confirmed the genetic background of the milk coagulability of sheep milk.					
35840399	10	27	theme	having	1594:1599	arg1	samples					1604:1610	having NC samples	1594:1610	having NC samples	1594:1610	Results of the present work demonstrated that the probability of having NC samples increases as milk contents of proteins and chlorides and somatic cell score increase.					
35840399	5	28	theme	milk	865:868	arg1	samples					870:876	Individual milk samples	854:876	Individual milk samples	854:876	Individual milk samples were collected from 996 Sarda ewes farmed in 47 flocks located in Sardinia (Italy).					
35840399	3	29	theme	coagulation	761:771	arg1	status					773:778	coagulation status	761:778	coagulation status	761:778	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	0	30	from	sheep	90:94	arg1	characterization					23:38	Phenotypic and genetic characterization	0:38	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.	0:95	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.					
35840399	11	31	theme	coagulation	1761:1771	arg1	status					1773:1778	coagulation status	1761:1778	coagulation status highlighted key regions associated with lactose and protein concentrations	1761:1853	The analysis of PC extracted from milk spectra that influenced coagulation status highlighted key regions associated with lactose and protein concentrations, and others not associated with routinely collected milk composition traits.					
35840399	13	32	theme	Genetic	2142:2148	arg1	analysis					2150:2157	Genetic analysis	2142:2157	Genetic analysis of milk coagulation status and of the extracted PC	2142:2208	Genetic analysis of milk coagulation status and of the extracted PC confirmed the genetic background of the milk coagulability of sheep milk.					
35840399	0	33	theme	noncoagulating	61:74	arg1	milk					76:79	noncoagulating milk	61:79	noncoagulating milk	61:79	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.					
35840399	12	34	theme	unhealthy	2110:2118	arg1	gland					2128:2132	unhealthy mammary gland	2110:2132	unhealthy mammary gland	2110:2132	These results suggest that the occurrence of NC is mostly related to damage of the epithelium secretory mammary cells, which occurs with the advancement of a lactation or due to unhealthy mammary gland status.					
35840399	1	35	theme	sheep	155:159	arg1	industry					167:174	the sheep dairy industry	151:174	the sheep dairy industry	151:174	Milk coagulation ability is of central importance for the sheep dairy industry because almost all sheep milk is destined for cheese processing.					
35840399	11	36	theme	protein	1832:1838	arg1	concentrations					1840:1853	protein concentrations	1832:1853	protein concentrations	1832:1853	The analysis of PC extracted from milk spectra that influenced coagulation status highlighted key regions associated with lactose and protein concentrations, and others not associated with routinely collected milk composition traits.					
35840399	3	37	from	effect	499:504	arg1	samples					610:616	milk coagulation status [coagulating and noncoagulating (NC) milk samples	544:616	samples	610:616	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	3	37	from	effect	499:504	arg1	[coagulating					568:579	[coagulating	568:579	[coagulating	568:579	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	5	38	theme	Sarda	902:906	arg1	ewes					908:911	996 Sarda ewes	898:911	996 Sarda ewes farmed in 47 flocks located in Sardinia (Italy)	898:959	Individual milk samples were collected from 996 Sarda ewes farmed in 47 flocks located in Sardinia (Italy).					
35840399	1	39	theme	central	128:134	arg1	importance					136:145	central importance	128:145	central importance for the sheep dairy industry	128:174	Milk coagulation ability is of central importance for the sheep dairy industry because almost all sheep milk is destined for cheese processing.					
35840399	0	40	from	occurrence	47:56	arg1	sheep					90:94	dairy sheep	84:94	dairy sheep	84:94	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.					
35840399	8	41	theme	regression	1220:1229	arg1	approach					1231:1238	A logistic regression approach	1209:1238	A logistic regression approach	1209:1238	A logistic regression approach was used to test the effect of milk-related traits on milk coagulation status.					
35840399	11	42	theme	milk	1732:1735	arg1	spectra					1737:1743	milk spectra	1732:1743	milk spectra that influenced coagulation status highlighted key regions associated with lactose and protein concentrations, and others not associated with routinely collected milk composition traits	1732:1929	The analysis of PC extracted from milk spectra that influenced coagulation status highlighted key regions associated with lactose and protein concentrations, and others not associated with routinely collected milk composition traits.					
35840399	5	43	theme	farmed	913:918	arg1	ewes					908:911	996 Sarda ewes	898:911	996 Sarda ewes farmed in 47 flocks located in Sardinia (Italy)	898:959	Individual milk samples were collected from 996 Sarda ewes farmed in 47 flocks located in Sardinia (Italy).					
35840399	6	44	theme	milk	991:994	arg1	yield					996:1000	daily milk yield	985:1000	daily milk yield	985:1000	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	12	45	theme	due	2103:2105	arg1	status					2134:2139	due to unhealthy mammary gland status	2103:2139	due to unhealthy mammary gland status	2103:2139	These results suggest that the occurrence of NC is mostly related to damage of the epithelium secretory mammary cells, which occurs with the advancement of a lactation or due to unhealthy mammary gland status.					
35840399	0	46	theme	Phenotypic	0:9	arg1	characterization					23:38	Phenotypic and genetic characterization	0:38	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.	0:95	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.					
35840399	6	47	dep	yield	996:1000	arg1	time					1099:1102	curd firming time	1086:1102	curd firming time	1086:1102	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	6	47	dep	yield	996:1000	arg1	firmness					1114:1121	curd firmness	1109:1121	curd firmness	1109:1121	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	6	47	dep	yield	996:1000	arg1	time					1080:1083	rennet coagulation time	1061:1083	rennet coagulation time	1061:1083	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	8	48	used	used	1244:1247	arg2	approach					1231:1238	A logistic regression approach	1209:1238	A logistic regression approach	1209:1238	A logistic regression approach was used to test the effect of milk-related traits on milk coagulation status.					
35840399	0	49	theme	genetic	15:21	arg1	characterization					23:38	Phenotypic and genetic characterization	0:38	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.	0:95	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.					
35840399	1	50	theme	coagulation	102:112	arg1	ability					114:120	Milk coagulation ability	97:120	Milk coagulation ability	97:120	Milk coagulation ability is of central importance for the sheep dairy industry because almost all sheep milk is destined for cheese processing.					
35840399	6	51	theme	Considered	962:971	arg1	traits					973:978	Considered traits	962:978	Considered traits	962:978	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	8	52	from	effect	1261:1266	arg1	status					1311:1316	milk coagulation status	1294:1316	milk coagulation status	1294:1316	A logistic regression approach was used to test the effect of milk-related traits on milk coagulation status.					
35840399	3	53	from	variability	746:756	arg1	status					773:778	coagulation status	761:778	coagulation status	761:778	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	12	54	theme	secretory	2026:2034	arg1	cells					2044:2048	the epithelium secretory mammary cells	2011:2048	the epithelium secretory mammary cells	2011:2048	These results suggest that the occurrence of NC is mostly related to damage of the epithelium secretory mammary cells, which occurs with the advancement of a lactation or due to unhealthy mammary gland status.					
35840399	2	55	theme	dairy	379:383	arg1	companies					385:393	the dairy companies	375:393	the dairy companies	375:393	The occurrence of milk with impaired coagulation properties is an obstacle to cheese processing and, in turn, to the profitability of the dairy companies.					
35840399	13	56	theme	milk	2162:2165	arg1	status					2179:2184	milk coagulation status	2162:2184	milk coagulation status	2162:2184	Genetic analysis of milk coagulation status and of the extracted PC confirmed the genetic background of the milk coagulability of sheep milk.					
35840399	7	57	theme	samples	1168:1174	arg1	samples					1168:1174	samples	1168:1174	samples	1168:1174	About 9% of samples did not coagulate within 30 min.					
35840399	7	57	theme	samples	1168:1174	arg1	%					1163:1163	About 9%	1156:1163	About 9% of samples	1156:1174	About 9% of samples did not coagulate within 30 min.					
35840399	10	58	theme	cell	1677:1680	arg1	score					1682:1686	somatic cell score	1669:1686	somatic cell score	1669:1686	Results of the present work demonstrated that the probability of having NC samples increases as milk contents of proteins and chlorides and somatic cell score increase.					
35840399	12	59	theme	cells	2044:2048	arg1	damage					2001:2006	damage	2001:2006	damage	2001:2006	These results suggest that the occurrence of NC is mostly related to damage of the epithelium secretory mammary cells, which occurs with the advancement of a lactation or due to unhealthy mammary gland status.					
35840399	0	60	theme	occurrence	47:56	arg1	characterization					23:38	Phenotypic and genetic characterization	0:38	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.	0:95	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.					
35840399	12	61	theme	mammary	2120:2126	arg1	gland					2128:2132	unhealthy mammary gland	2110:2132	unhealthy mammary gland	2110:2132	These results suggest that the occurrence of NC is mostly related to damage of the epithelium secretory mammary cells, which occurs with the advancement of a lactation or due to unhealthy mammary gland status.					
35840399	6	62	theme	milk	1032:1035	arg1	properties					1049:1058	milk coagulation properties	1032:1058	milk coagulation properties	1032:1058	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	8	63	theme	traits	1284:1289	arg1	effect					1261:1266	the effect	1257:1266	the effect of milk-related traits on milk coagulation status	1257:1316	A logistic regression approach was used to test the effect of milk-related traits on milk coagulation status.					
35840399	2	64	theme	cheese	319:324	arg1	processing					326:335	cheese processing	319:335	cheese processing	319:335	The occurrence of milk with impaired coagulation properties is an obstacle to cheese processing and, in turn, to the profitability of the dairy companies.					
35840399	9	65	theme	logistic	1444:1451	arg1	model					1464:1468	a logistic regression model	1442:1468	a logistic regression model to assess their relationship with milk coagulation status	1442:1526	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	8	66	theme	milk	1294:1297	arg1	status					1311:1316	milk coagulation status	1294:1316	milk coagulation status	1294:1316	A logistic regression approach was used to test the effect of milk-related traits on milk coagulation status.					
35840399	1	67	theme	cheese	222:227	arg1	processing					229:238	cheese processing	222:238	cheese processing	222:238	Milk coagulation ability is of central importance for the sheep dairy industry because almost all sheep milk is destined for cheese processing.					
35840399	5	68	theme	located	933:939	arg1	flocks					926:931	47 flocks	923:931	47 flocks located in Sardinia (Italy)	923:959	Individual milk samples were collected from 996 Sarda ewes farmed in 47 flocks located in Sardinia (Italy).					
35840399	6	69	theme	composition	1008:1018	arg1	traits					1020:1025	milk composition traits	1003:1025	milk composition traits	1003:1025	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	3	70	theme	milk	464:467	arg1	noncoagulation					440:453	noncoagulation	440:453	noncoagulation of sheep milk	440:467	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	5	71	from	farmed	913:918	arg1	flocks					926:931	47 flocks	923:931	47 flocks located in Sardinia (Italy)	923:959	Individual milk samples were collected from 996 Sarda ewes farmed in 47 flocks located in Sardinia (Italy).					
35840399	13	72	theme	coagulability	2255:2267	arg1	background					2232:2241	the genetic background	2220:2241	the genetic background of the milk coagulability of sheep milk	2220:2281	Genetic analysis of milk coagulation status and of the extracted PC confirmed the genetic background of the milk coagulability of sheep milk.					
35840399	2	73	theme	coagulation	278:288	arg1	properties					290:299	impaired coagulation properties	269:299	impaired coagulation properties	269:299	The occurrence of milk with impaired coagulation properties is an obstacle to cheese processing and, in turn, to the profitability of the dairy companies.					
35840399	6	74	theme	curd	1086:1089	arg1	time					1099:1102	curd firming time	1086:1102	curd firming time	1086:1102	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	5	75	from	located	933:939	arg1	Sardinia					944:951	Sardinia	944:951	Sardinia (Italy)	944:959	Individual milk samples were collected from 996 Sarda ewes farmed in 47 flocks located in Sardinia (Italy).					
35840399	5	75	from	located	933:939	arg1	Italy					954:958	Italy	954:958	Italy	954:958	Individual milk samples were collected from 996 Sarda ewes farmed in 47 flocks located in Sardinia (Italy).					
35840399	1	76	theme	dairy	161:165	arg1	industry					167:174	the sheep dairy industry	151:174	the sheep dairy industry	151:174	Milk coagulation ability is of central importance for the sheep dairy industry because almost all sheep milk is destined for cheese processing.					
35840399	9	77	theme	milk	1504:1507	arg1	status					1521:1526	milk coagulation status	1504:1526	milk coagulation status	1504:1526	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	12	78	theme	NC	1977:1978	arg1	occurrence					1963:1972	the occurrence	1959:1972	the occurrence of NC	1959:1978	These results suggest that the occurrence of NC is mostly related to damage of the epithelium secretory mammary cells, which occurs with the advancement of a lactation or due to unhealthy mammary gland status.					
35840399	12	78	theme	NC	1977:1978	arg1	related					1990:1996	related	1990:1996	related	1990:1996	These results suggest that the occurrence of NC is mostly related to damage of the epithelium secretory mammary cells, which occurs with the advancement of a lactation or due to unhealthy mammary gland status.					
35840399	3	79	theme	physicochemical	514:528	arg1	properties					530:539	milk physicochemical properties	509:539	milk physicochemical properties	509:539	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	13	80	theme	status	2179:2184	arg1	analysis					2150:2157	Genetic analysis	2142:2157	Genetic analysis of milk coagulation status and of the extracted PC	2142:2208	Genetic analysis of milk coagulation status and of the extracted PC confirmed the genetic background of the milk coagulability of sheep milk.					
35840399	12	81	theme	status	2134:2139	arg1	advancement					2073:2083	the advancement	2069:2083	the advancement of a lactation or due to unhealthy mammary gland status	2069:2139	These results suggest that the occurrence of NC is mostly related to damage of the epithelium secretory mammary cells, which occurs with the advancement of a lactation or due to unhealthy mammary gland status.					
35840399	6	82	theme	rennet	1061:1066	arg1	time					1080:1083	rennet coagulation time	1061:1083	rennet coagulation time	1061:1083	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	10	83	theme	samples	1604:1610	arg1	probability					1579:1589	the probability	1575:1589	the probability of having NC samples	1575:1610	Results of the present work demonstrated that the probability of having NC samples increases as milk contents of proteins and chlorides and somatic cell score increase.					
35840399	1	84	theme	all	191:193	arg1	milk					201:204	almost all sheep milk	184:204	almost all sheep milk	184:204	Milk coagulation ability is of central importance for the sheep dairy industry because almost all sheep milk is destined for cheese processing.					
35840399	4	85	theme	ability	845:851	arg1	background					814:823	the genetic background	802:823	the genetic background of milk coagulation ability	802:851	We also investigated the genetic background of milk coagulation ability.					
35840399	9	86	theme	milk	1378:1381	arg1	spectra					1387:1393	the milk MIR spectra	1374:1393	the milk MIR spectra	1374:1393	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	10	87	theme	proteins	1642:1649	arg1	contents					1630:1637	milk contents	1625:1637	milk contents of proteins and chlorides	1625:1663	Results of the present work demonstrated that the probability of having NC samples increases as milk contents of proteins and chlorides and somatic cell score increase.					
35840399	10	87	theme	proteins	1642:1649	arg1	score					1682:1686	somatic cell score	1669:1686	somatic cell score	1669:1686	Results of the present work demonstrated that the probability of having NC samples increases as milk contents of proteins and chlorides and somatic cell score increase.					
35840399	5	88	from	Sardinia	944:951	arg1	located					933:939	located	933:939	located	933:939	Individual milk samples were collected from 996 Sarda ewes farmed in 47 flocks located in Sardinia (Italy).					
35840399	11	89	theme	milk	1907:1910	arg1	traits					1924:1929	routinely collected milk composition traits	1887:1929	routinely collected milk composition traits	1887:1929	The analysis of PC extracted from milk spectra that influenced coagulation status highlighted key regions associated with lactose and protein concentrations, and others not associated with routinely collected milk composition traits.					
35840399	3	90	theme	noncoagulating	585:598	arg1	samples					610:616	milk coagulation status [coagulating and noncoagulating (NC) milk samples	544:616	samples	610:616	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	5	91	theme	Individual	854:863	arg1	samples					870:876	Individual milk samples	854:876	Individual milk samples	854:876	Individual milk samples were collected from 996 Sarda ewes farmed in 47 flocks located in Sardinia (Italy).					
35840399	4	92	theme	milk	828:831	arg1	ability					845:851	milk coagulation ability	828:851	milk coagulation ability	828:851	We also investigated the genetic background of milk coagulation ability.					
35840399	0	93	theme	milk	76:79	arg1	occurrence					47:56	the occurrence	43:56	the occurrence of noncoagulating milk in dairy sheep	43:94	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.					
35840399	10	94	theme	chlorides	1655:1663	arg1	contents					1630:1637	milk contents	1625:1637	milk contents of proteins and chlorides	1625:1663	Results of the present work demonstrated that the probability of having NC samples increases as milk contents of proteins and chlorides and somatic cell score increase.					
35840399	10	94	theme	chlorides	1655:1663	arg1	score					1682:1686	somatic cell score	1669:1686	somatic cell score	1669:1686	Results of the present work demonstrated that the probability of having NC samples increases as milk contents of proteins and chlorides and somatic cell score increase.					
35840399	9	95	theme	principal	1321:1329	arg1	component					1331:1339	A principal component	1319:1339	A principal component (PC) analysis	1319:1353	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	0	96	from	characterization	23:38	arg1	sheep					90:94	dairy sheep	84:94	dairy sheep	84:94	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.					
35840399	0	97	theme	dairy	84:88	arg1	sheep					90:94	dairy sheep	84:94	dairy sheep	84:94	Phenotypic and genetic characterization of the occurrence of noncoagulating milk in dairy sheep.					
35840399	3	98	used	used	731:734	arg2	MIR					717:719	MIR	717:719	MIR	717:719	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	3	98	used	used	731:734	arg2	spectroscopy					703:714	mid-infrared spectroscopy	690:714	mid-infrared spectroscopy (MIR)	690:720	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	9	99	theme	MIR	1383:1385	arg1	spectra					1387:1393	the milk MIR spectra	1374:1393	the milk MIR spectra	1374:1393	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	10	100	theme	NC	1601:1602	arg1	samples					1604:1610	having NC samples	1594:1610	having NC samples	1594:1610	Results of the present work demonstrated that the probability of having NC samples increases as milk contents of proteins and chlorides and somatic cell score increase.					
35840399	13	101	theme	sheep	2272:2276	arg1	milk					2278:2281	sheep milk	2272:2281	sheep milk	2272:2281	Genetic analysis of milk coagulation status and of the extracted PC confirmed the genetic background of the milk coagulability of sheep milk.					
35840399	9	102	theme	component	1331:1339	arg1	analysis					1346:1353	A principal component (PC) analysis	1319:1353	A principal component (PC) analysis	1319:1353	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	9	102	theme	component	1331:1339	arg1	PC					1342:1343	PC	1342:1343	PC	1342:1343	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	13	103	theme	milk	2278:2281	arg1	coagulability					2255:2267	the milk coagulability	2246:2267	the milk coagulability of sheep milk	2246:2281	Genetic analysis of milk coagulation status and of the extracted PC confirmed the genetic background of the milk coagulability of sheep milk.					
35840399	8	104	theme	logistic	1211:1218	arg1	approach					1231:1238	A logistic regression approach	1209:1238	A logistic regression approach	1209:1238	A logistic regression approach was used to test the effect of milk-related traits on milk coagulation status.					
35840399	9	105	theme	PC	1400:1401	arg1	covariates					1428:1437	covariates	1428:1437	covariates	1428:1437	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	9	105	theme	PC	1400:1401	arg1	scores					1403:1408	PC scores	1400:1408	PC scores	1400:1408	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	12	106	theme	lactation	2090:2098	arg1	advancement					2073:2083	the advancement	2069:2083	the advancement of a lactation or due to unhealthy mammary gland status	2069:2139	These results suggest that the occurrence of NC is mostly related to damage of the epithelium secretory mammary cells, which occurs with the advancement of a lactation or due to unhealthy mammary gland status.					
35840399	6	107	theme	daily	985:989	arg1	yield					996:1000	daily milk yield	985:1000	daily milk yield	985:1000	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	13	108	theme	extracted	2197:2205	arg1	PC					2207:2208	the extracted PC	2193:2208	the extracted PC	2193:2208	Genetic analysis of milk coagulation status and of the extracted PC confirmed the genetic background of the milk coagulability of sheep milk.					
35840399	1	109	theme	Milk	97:100	arg1	ability					114:120	Milk coagulation ability	97:120	Milk coagulation ability	97:120	Milk coagulation ability is of central importance for the sheep dairy industry because almost all sheep milk is destined for cheese processing.					
35840399	3	110	theme	milk	605:608	arg1	samples					610:616	milk coagulation status [coagulating and noncoagulating (NC) milk samples	544:616	samples	610:616	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	2	111	theme	companies	385:393	arg1	profitability					358:370	the profitability	354:370	the profitability of the dairy companies	354:393	The occurrence of milk with impaired coagulation properties is an obstacle to cheese processing and, in turn, to the profitability of the dairy companies.					
35840399	3	112	theme	NC	601:602	arg1	samples					610:616	milk coagulation status [coagulating and noncoagulating (NC) milk samples	544:616	samples	610:616	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	12	113	theme	epithelium	2015:2024	arg1	cells					2044:2048	the epithelium secretory mammary cells	2011:2048	the epithelium secretory mammary cells	2011:2048	These results suggest that the occurrence of NC is mostly related to damage of the epithelium secretory mammary cells, which occurs with the advancement of a lactation or due to unhealthy mammary gland status.					
35840399	13	114	theme	genetic	2224:2230	arg1	background					2232:2241	the genetic background	2220:2241	the genetic background of the milk coagulability of sheep milk	2220:2281	Genetic analysis of milk coagulation status and of the extracted PC confirmed the genetic background of the milk coagulability of sheep milk.					
35840399	6	115	theme	coagulation	1037:1047	arg1	properties					1049:1058	milk coagulation properties	1032:1058	milk coagulation properties	1032:1058	Considered traits were daily milk yield, milk composition traits, and milk coagulation properties (rennet coagulation time, curd firming time, and curd firmness), and MIR spectra were acquired.					
35840399	12	116	theme	mammary	2036:2042	arg1	cells					2044:2048	the epithelium secretory mammary cells	2011:2048	the epithelium secretory mammary cells	2011:2048	These results suggest that the occurrence of NC is mostly related to damage of the epithelium secretory mammary cells, which occurs with the advancement of a lactation or due to unhealthy mammary gland status.					
35840399	3	117	theme	sheep	458:462	arg1	milk					464:467	sheep milk	458:467	sheep milk	458:467	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	13	118	theme	PC	2207:2208	arg1	analysis					2150:2157	Genetic analysis	2142:2157	Genetic analysis of milk coagulation status and of the extracted PC	2142:2208	Genetic analysis of milk coagulation status and of the extracted PC confirmed the genetic background of the milk coagulability of sheep milk.					
35840399	2	119	from	profitability	358:370	arg1	turn					345:348	turn	345:348	turn	345:348	The occurrence of milk with impaired coagulation properties is an obstacle to cheese processing and, in turn, to the profitability of the dairy companies.					
35840399	8	120	theme	milk-related	1271:1282	arg1	traits					1284:1289	milk-related traits	1271:1289	milk-related traits	1271:1289	A logistic regression approach was used to test the effect of milk-related traits on milk coagulation status.					
35840399	3	121	theme	noncoagulation	440:453	arg1	causes					430:435	the causes	426:435	the causes of noncoagulation of sheep milk	426:467	In this work, we investigated the causes of noncoagulation of sheep milk; specifically, we studied the effect of milk physicochemical properties on milk coagulation status [coagulating and noncoagulating (NC) milk samples, which do or do not coagulate within 30 min, respectively], and whether mid-infrared spectroscopy (MIR) could be used to assess variability in coagulation status.					
35840399	9	122	theme	regression	1453:1462	arg1	model					1464:1468	a logistic regression model	1442:1468	a logistic regression model to assess their relationship with milk coagulation status	1442:1526	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
35840399	10	123	theme	work	1552:1555	arg1	Results					1529:1535	Results	1529:1535	Results of the present work	1529:1555	Results of the present work demonstrated that the probability of having NC samples increases as milk contents of proteins and chlorides and somatic cell score increase.					
35840399	9	124	with	relationship	1486:1497	arg1	status					1521:1526	milk coagulation status	1504:1526	milk coagulation status	1504:1526	A principal component (PC) analysis was carried out on the milk MIR spectra, and PC scores were then used as covariates in a logistic regression model to assess their relationship with milk coagulation status.					
37239867	6	0	theme	mineral	811:817	arg1	deposition					819:828	mineral deposition	811:828	mineral deposition	811:828	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	2	1	theme	research	340:347	arg1	scaffolds					300:308	synthetic cell-free functionalized scaffolds	265:308	synthetic cell-free functionalized scaffolds	265:308	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	2	1	theme	research	340:347	arg1	focus					324:328	the focus	320:328	the focus of recent research in designing scaffolds for bone tissue engineering	320:398	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	8	2	theme	bovine	1049:1054	arg1	bone					1067:1070	bovine cancellous bone	1049:1070	bovine cancellous bone	1049:1070	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	4	3	theme	bone	553:556	arg1	repair					558:563	bone repair	553:563	bone repair	553:563	It has good biocompatibility, but few studies have investigated its use in bone repair.					
37239867	10	4	theme	effective	1446:1454	arg1	support					1467:1473	effective structural support	1446:1473	effective structural support	1446:1473	Administered into skull defects of rats, the scaffolds showed perfect bone-binding performance and effective structural support, and significantly promoted the regeneration of new bone.					
37239867	2	5	theme	synthetic	265:273	arg1	scaffolds					300:308	synthetic cell-free functionalized scaffolds	265:308	synthetic cell-free functionalized scaffolds	265:308	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	2	5	theme	synthetic	265:273	arg1	focus					324:328	the focus	320:328	the focus of recent research in designing scaffolds for bone tissue engineering	320:398	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	9	6	theme	mechanical	1293:1302	arg1	strength					1304:1311	mechanical strength	1293:1311	mechanical strength	1293:1311	With the aim of combining the advantages of BC and HA, a BC-HA composite scaffold, with a good pore structure and mechanical strength, was prepared by physical mixing.					
37239867	10	7	theme	bone	1527:1530	arg1	regeneration					1507:1518	the regeneration	1503:1518	the regeneration of new bone	1503:1530	Administered into skull defects of rats, the scaffolds showed perfect bone-binding performance and effective structural support, and significantly promoted the regeneration of new bone.					
37239867	8	8	theme	osteoblast	1148:1157	arg1	MC3T3-E1					1169:1176	the mouse osteoblast cell line MC3T3-E1	1138:1176	the mouse osteoblast cell line MC3T3-E1	1138:1176	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	7	9	theme	good	984:987	arg1	biocompatibility					989:1004	the good biocompatibility	980:1004	the good biocompatibility of BC	980:1010	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	6	10	theme	strong	712:717	arg1	N					749:749	47.8 ± 4.54 N	737:749	47.8 ± 4.54 N	737:749	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	6	10	theme	strong	712:717	arg1	strength					727:734	strong tensile strength	712:734	strong tensile strength (47.8 ± 4.54 N)	712:750	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	8	11	theme	cytocompatibility	1082:1098	arg1	activity					1125:1132	good cytocompatibility and osteogenic induction activity	1077:1132	good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1	1077:1176	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	8	12	theme	line	1164:1167	arg1	MC3T3-E1					1169:1176	the mouse osteoblast cell line MC3T3-E1	1138:1176	the mouse osteoblast cell line MC3T3-E1	1138:1176	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	2	13	theme	functionalized	285:298	arg1	scaffolds					300:308	synthetic cell-free functionalized scaffolds	265:308	synthetic cell-free functionalized scaffolds	265:308	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	2	13	theme	functionalized	285:298	arg1	focus					324:328	the focus	320:328	the focus of recent research in designing scaffolds for bone tissue engineering	320:398	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	9	14	theme	physical	1330:1337	arg1	mixing					1339:1344	physical mixing	1330:1344	physical mixing	1330:1344	With the aim of combining the advantages of BC and HA, a BC-HA composite scaffold, with a good pore structure and mechanical strength, was prepared by physical mixing.					
37239867	8	15	theme	osteogenic	1104:1113	arg1	induction					1115:1123	osteogenic induction	1104:1123	osteogenic induction	1104:1123	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	11	16	theme	bone	1702:1705	arg1	transplantation					1707:1721	bone transplantation	1702:1721	bone transplantation	1702:1721	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	1	17	theme	human	199:203	arg1	health					205:210	human health	199:210	human health	199:210	Bone defects, a common orthopedic problem in clinical practice, are a serious threat to human health.					
37239867	5	18	theme	substitution	630:641	arg1	degree					620:625	a degree	618:625	a degree of substitution of 2.1	618:648	In this study, BC was successfully synthesized with a degree of substitution of 2.1.					
37239867	2	19	theme	autologous	241:250	arg1	grafts					257:262	alternative materials to autologous bone grafts	216:262	alternative materials to autologous bone grafts	216:262	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	1	20	theme	common	127:132	arg1	problem					145:151	a common orthopedic problem	125:151	a common orthopedic problem in clinical practice	125:172	Bone defects, a common orthopedic problem in clinical practice, are a serious threat to human health.					
37239867	1	20	theme	common	127:132	arg1	defects					116:122	Bone defects	111:122	Bone defects	111:122	Bone defects, a common orthopedic problem in clinical practice, are a serious threat to human health.					
37239867	11	21	theme	engineering	1612:1622	arg1	scaffold					1575:1582	the BC-HA porous scaffold	1558:1582	the BC-HA porous scaffold	1558:1582	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	11	21	theme	engineering	1612:1622	arg1	scaffold					1624:1631	a successful bone tissue engineering scaffold	1587:1631	a successful bone tissue engineering scaffold	1587:1631	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	8	22	contain	had	1073:1075	arg2	activity					1125:1132	good cytocompatibility and osteogenic induction activity	1077:1132	good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1	1077:1176	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	8	22	contain	had	1073:1075	arg1	HA					1029:1030	HA	1029:1030	HA	1029:1030	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	8	22	contain	had	1073:1075	arg1	Hydroxyapatite					1013:1026	Hydroxyapatite	1013:1026	Hydroxyapatite (HA)	1013:1031	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	7	23	theme	excellent	875:883	arg1	attachment					890:899	the excellent cell attachment	871:899	the excellent cell attachment	871:899	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	0	24	theme	Cranial	83:89	arg1	Repair					103:108	Cranial Bone Defect Repair	83:108	Cranial Bone Defect Repair	83:108	A Novel Porous Butyryl Chitin-Animal Derived Hydroxyapatite Composite Scaffold for Cranial Bone Defect Repair.					
37239867	1	25	theme	clinical	156:163	arg1	practice					165:172	clinical practice	156:172	clinical practice	156:172	Bone defects, a common orthopedic problem in clinical practice, are a serious threat to human health.					
37239867	11	26	theme	strong	1641:1646	arg1	potential					1648:1656	strong potential	1641:1656	strong potential to be further developed as a substitute for bone transplantation	1641:1721	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	0	27	theme	Defect	96:101	arg1	Repair					103:108	Cranial Bone Defect Repair	83:108	Cranial Bone Defect Repair	83:108	A Novel Porous Butyryl Chitin-Animal Derived Hydroxyapatite Composite Scaffold for Cranial Bone Defect Repair.					
37239867	7	28	theme	cytological	843:853	arg1	assay					855:859	An in vitro cytological assay	831:859	An in vitro cytological assay	831:859	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	6	29	theme	BC	651:652	arg1	films					654:658	BC films	651:658	BC films	651:658	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	7	30	theme	in	834:835	arg1	assay					855:859	An in vitro cytological assay	831:859	An in vitro cytological assay	831:859	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	2	31	theme	bone	376:379	arg1	engineering					388:398	bone tissue engineering	376:398	bone tissue engineering	376:398	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	9	32	theme	BC	1223:1224	arg1	advantages					1209:1218	the advantages	1205:1218	the advantages of BC and HA, a BC-HA composite scaffold	1205:1259	With the aim of combining the advantages of BC and HA, a BC-HA composite scaffold, with a good pore structure and mechanical strength, was prepared by physical mixing.					
37239867	4	33	theme	few	512:514	arg1	studies					516:522	few studies	512:522	few studies	512:522	It has good biocompatibility, but few studies have investigated its use in bone repair.					
37239867	3	34	theme	chitin	440:445	arg1	chitin					409:414	Butyryl chitin	401:414	Butyryl chitin (BC)	401:419	Butyryl chitin (BC) is a derivative of chitin (CT) with improved solubility.					
37239867	3	34	theme	chitin	440:445	arg1	derivative					426:435	a derivative	424:435	a derivative of chitin (CT) with improved solubility	424:475	Butyryl chitin (BC) is a derivative of chitin (CT) with improved solubility.					
37239867	0	35	theme	Porous	8:13	arg1	Chitin-Animal					23:35	A Novel Porous Butyryl Chitin-Animal	0:35	A Novel Porous Butyryl Chitin-Animal	0:35	A Novel Porous Butyryl Chitin-Animal Derived Hydroxyapatite Composite Scaffold for Cranial Bone Defect Repair.					
37239867	9	36	theme	HA	1230:1231	arg1	advantages					1209:1218	the advantages	1205:1218	the advantages of BC and HA, a BC-HA composite scaffold	1205:1259	With the aim of combining the advantages of BC and HA, a BC-HA composite scaffold, with a good pore structure and mechanical strength, was prepared by physical mixing.					
37239867	1	37	theme	Bone	111:114	arg1	problem					145:151	a common orthopedic problem	125:151	a common orthopedic problem in clinical practice	125:172	Bone defects, a common orthopedic problem in clinical practice, are a serious threat to human health.					
37239867	1	37	theme	Bone	111:114	arg1	defects					116:122	Bone defects	111:122	Bone defects	111:122	Bone defects, a common orthopedic problem in clinical practice, are a serious threat to human health.					
37239867	1	37	theme	Bone	111:114	arg1	threat					189:194	a serious threat	179:194	a serious threat to human health	179:210	Bone defects, a common orthopedic problem in clinical practice, are a serious threat to human health.					
37239867	6	38	dep	showed	705:710	arg1	favorable					797:805	favorable	797:805	favorable	797:805	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	4	39	theme	good	485:488	arg1	biocompatibility					490:505	good biocompatibility	485:505	good biocompatibility	485:505	It has good biocompatibility, but few studies have investigated its use in bone repair.					
37239867	11	40	theme	porous	1568:1573	arg1	scaffold					1575:1582	the BC-HA porous scaffold	1558:1582	the BC-HA porous scaffold	1558:1582	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	11	40	theme	porous	1568:1573	arg1	scaffold					1624:1631	a successful bone tissue engineering scaffold	1587:1631	a successful bone tissue engineering scaffold	1587:1631	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	2	41	theme	recent	333:338	arg1	research					340:347	recent research	333:347	recent research in designing scaffolds for bone tissue engineering	333:398	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	0	42	theme	Hydroxyapatite	45:58	arg1	Scaffold					70:77	Hydroxyapatite Composite Scaffold	45:77	Hydroxyapatite Composite Scaffold for Cranial Bone Defect Repair	45:108	A Novel Porous Butyryl Chitin-Animal Derived Hydroxyapatite Composite Scaffold for Cranial Bone Defect Repair.					
37239867	9	43	theme	composite	1242:1250	arg1	BC					1223:1224	BC	1223:1224	BC	1223:1224	With the aim of combining the advantages of BC and HA, a BC-HA composite scaffold, with a good pore structure and mechanical strength, was prepared by physical mixing.					
37239867	9	43	theme	composite	1242:1250	arg1	scaffold					1252:1259	a BC-HA composite scaffold	1234:1259	a BC-HA composite scaffold	1234:1259	With the aim of combining the advantages of BC and HA, a BC-HA composite scaffold, with a good pore structure and mechanical strength, was prepared by physical mixing.					
37239867	2	44	theme	materials	228:236	arg1	grafts					257:262	alternative materials to autologous bone grafts	216:262	alternative materials to autologous bone grafts	216:262	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	10	45	theme	rats	1382:1385	arg1	defects					1371:1377	skull defects	1365:1377	skull defects of rats	1365:1385	Administered into skull defects of rats, the scaffolds showed perfect bone-binding performance and effective structural support, and significantly promoted the regeneration of new bone.					
37239867	11	46	theme	tissue	1605:1610	arg1	engineering					1612:1622	a successful bone tissue engineering	1587:1622	a successful bone tissue engineering scaffold	1587:1631	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	6	47	theme	cast	684:687	arg1	method					694:699	the cast film method	680:699	the cast film method	680:699	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	10	48	theme	bone-binding	1417:1428	arg1	performance					1430:1440	perfect bone-binding performance	1409:1440	perfect bone-binding performance	1409:1440	Administered into skull defects of rats, the scaffolds showed perfect bone-binding performance and effective structural support, and significantly promoted the regeneration of new bone.					
37239867	11	49	theme	successful	1589:1598	arg1	engineering					1612:1622	a successful bone tissue engineering	1587:1622	a successful bone tissue engineering scaffold	1587:1631	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	9	50	theme	pore	1274:1277	arg1	structure					1279:1287	a good pore structure	1267:1287	a good pore structure	1267:1287	With the aim of combining the advantages of BC and HA, a BC-HA composite scaffold, with a good pore structure and mechanical strength, was prepared by physical mixing.					
37239867	10	51	theme	structural	1456:1465	arg1	support					1467:1473	effective structural support	1446:1473	effective structural support	1446:1473	Administered into skull defects of rats, the scaffolds showed perfect bone-binding performance and effective structural support, and significantly promoted the regeneration of new bone.					
37239867	7	52	theme	BC	1009:1010	arg1	biocompatibility					989:1004	the good biocompatibility	980:1004	the good biocompatibility of BC	980:1010	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	6	53	theme	±	742:742	arg1	N					749:749	47.8 ± 4.54 N	737:749	47.8 ± 4.54 N	737:749	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	6	53	theme	±	742:742	arg1	strength					727:734	strong tensile strength	712:734	strong tensile strength (47.8 ± 4.54 N)	712:750	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	2	54	theme	cell-free	275:283	arg1	scaffolds					300:308	synthetic cell-free functionalized scaffolds	265:308	synthetic cell-free functionalized scaffolds	265:308	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	2	54	theme	cell-free	275:283	arg1	focus					324:328	the focus	320:328	the focus of recent research in designing scaffolds for bone tissue engineering	320:398	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	7	55	dep	meanwhile	939:947	arg1	in					950:951	in	950:951	in	950:951	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	8	56	theme	cancellous	1056:1065	arg1	bone					1067:1070	bovine cancellous bone	1049:1070	bovine cancellous bone	1049:1070	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	6	57	theme	tensile	719:725	arg1	N					749:749	47.8 ± 4.54 N	737:749	47.8 ± 4.54 N	737:749	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	6	57	theme	tensile	719:725	arg1	strength					727:734	strong tensile strength	712:734	strong tensile strength (47.8 ± 4.54 N)	712:750	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	11	58	contain	has	1637:1639	arg1	scaffold					1575:1582	the BC-HA porous scaffold	1558:1582	the BC-HA porous scaffold	1558:1582	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	11	58	contain	has	1637:1639	arg2	potential					1648:1656	strong potential	1641:1656	strong potential to be further developed as a substitute for bone transplantation	1641:1721	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	11	58	contain	has	1637:1639	arg1	scaffold					1624:1631	a successful bone tissue engineering scaffold	1587:1631	a successful bone tissue engineering scaffold	1587:1631	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	8	59	theme	mouse	1142:1146	arg1	MC3T3-E1					1169:1176	the mouse osteoblast cell line MC3T3-E1	1138:1176	the mouse osteoblast cell line MC3T3-E1	1138:1176	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	3	60	with	derivative	426:435	arg1	solubility					466:475	improved solubility	457:475	improved solubility	457:475	Butyryl chitin (BC) is a derivative of chitin (CT) with improved solubility.					
37239867	1	61	theme	serious	181:187	arg1	threat					189:194	a serious threat	179:194	a serious threat to human health	179:210	Bone defects, a common orthopedic problem in clinical practice, are a serious threat to human health.					
37239867	1	61	theme	serious	181:187	arg1	defects					116:122	Bone defects	111:122	Bone defects	111:122	Bone defects, a common orthopedic problem in clinical practice, are a serious threat to human health.					
37239867	8	62	theme	good	1077:1080	arg1	activity					1125:1132	good cytocompatibility and osteogenic induction activity	1077:1132	good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1	1077:1176	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	8	63	theme	cell	1159:1162	arg1	MC3T3-E1					1169:1176	the mouse osteoblast cell line MC3T3-E1	1138:1176	the mouse osteoblast cell line MC3T3-E1	1138:1176	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	2	64	theme	alternative	216:226	arg1	grafts					257:262	alternative materials to autologous bone grafts	216:262	alternative materials to autologous bone grafts	216:262	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	7	65	theme	meanwhile	939:947	arg1	degradation					958:968	meanwhile, in vivo degradation	939:968	meanwhile, in vivo degradation	939:968	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	7	66	theme	BC	930:931	arg1	film					933:936	the BC film	926:936	the BC film	926:936	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	8	67	theme	induction	1115:1123	arg1	activity					1125:1132	good cytocompatibility and osteogenic induction activity	1077:1132	good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1	1077:1176	Hydroxyapatite (HA), extracted from bovine cancellous bone, had good cytocompatibility and osteogenic induction activity for the mouse osteoblast cell line MC3T3-E1.					
37239867	5	68	theme	2.1	646:648	arg1	substitution					630:641	substitution	630:641	substitution of 2.1	630:648	In this study, BC was successfully synthesized with a degree of substitution of 2.1.					
37239867	7	69	theme	film	933:936	arg1	attachment					890:899	the excellent cell attachment	871:899	the excellent cell attachment	871:899	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	7	69	theme	film	933:936	arg1	cytocompatibility					905:921	cytocompatibility	905:921	cytocompatibility	905:921	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	2	70	theme	bone	252:255	arg1	grafts					257:262	alternative materials to autologous bone grafts	216:262	alternative materials to autologous bone grafts	216:262	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	1	71	theme	orthopedic	134:143	arg1	problem					145:151	a common orthopedic problem	125:151	a common orthopedic problem in clinical practice	125:172	Bone defects, a common orthopedic problem in clinical practice, are a serious threat to human health.					
37239867	1	71	theme	orthopedic	134:143	arg1	defects					116:122	Bone defects	111:122	Bone defects	111:122	Bone defects, a common orthopedic problem in clinical practice, are a serious threat to human health.					
37239867	2	72	dep	grafts	257:262	arg1	to					238:239	to	238:239	to	238:239	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	0	73	theme	Bone	91:94	arg1	Repair					103:108	Cranial Bone Defect Repair	83:108	Cranial Bone Defect Repair	83:108	A Novel Porous Butyryl Chitin-Animal Derived Hydroxyapatite Composite Scaffold for Cranial Bone Defect Repair.					
37239867	7	74	theme	cell	885:888	arg1	attachment					890:899	the excellent cell attachment	871:899	the excellent cell attachment	871:899	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	7	75	dep	in	834:835	arg1	vitro					837:841	vitro	837:841	vitro	837:841	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	11	76	dep	a	1685:1685	arg1	substitute					1687:1696	substitute	1687:1696	substitute for bone transplantation	1687:1721	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	4	77	from	use	546:548	arg1	repair					558:563	bone repair	553:563	bone repair	553:563	It has good biocompatibility, but few studies have investigated its use in bone repair.					
37239867	1	78	from	problem	145:151	arg1	practice					165:172	clinical practice	156:172	clinical practice	156:172	Bone defects, a common orthopedic problem in clinical practice, are a serious threat to human health.					
37239867	2	79	theme	tissue	381:386	arg1	engineering					388:398	bone tissue engineering	376:398	bone tissue engineering	376:398	As alternative materials to autologous bone grafts, synthetic cell-free functionalized scaffolds have been the focus of recent research in designing scaffolds for bone tissue engineering.					
37239867	0	80	theme	Novel	2:6	arg1	Chitin-Animal					23:35	A Novel Porous Butyryl Chitin-Animal	0:35	A Novel Porous Butyryl Chitin-Animal	0:35	A Novel Porous Butyryl Chitin-Animal Derived Hydroxyapatite Composite Scaffold for Cranial Bone Defect Repair.					
37239867	7	81	dep	in	950:951	arg1	vivo					953:956	vivo	953:956	vivo	953:956	An in vitro cytological assay confirmed the excellent cell attachment and cytocompatibility of the BC film; meanwhile, in vivo degradation indicated the good biocompatibility of BC.					
37239867	10	82	theme	new	1523:1525	arg1	bone					1527:1530	new bone	1523:1530	new bone	1523:1530	Administered into skull defects of rats, the scaffolds showed perfect bone-binding performance and effective structural support, and significantly promoted the regeneration of new bone.					
37239867	0	83	theme	Butyryl	15:21	arg1	Chitin-Animal					23:35	A Novel Porous Butyryl Chitin-Animal	0:35	A Novel Porous Butyryl Chitin-Animal	0:35	A Novel Porous Butyryl Chitin-Animal Derived Hydroxyapatite Composite Scaffold for Cranial Bone Defect Repair.					
37239867	10	84	theme	skull	1365:1369	arg1	defects					1371:1377	skull defects	1365:1377	skull defects of rats	1365:1385	Administered into skull defects of rats, the scaffolds showed perfect bone-binding performance and effective structural support, and significantly promoted the regeneration of new bone.					
37239867	6	85	theme	±	777:777	arg1	hydrophobicity					756:769	hydrophobicity	756:769	hydrophobicity (86.4 ± 2.46°)	756:784	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	6	85	theme	±	777:777	arg1	2.46°					779:783	86.4 ± 2.46°	772:783	86.4 ± 2.46°	772:783	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	11	86	theme	BC-HA	1562:1566	arg1	scaffold					1575:1582	the BC-HA porous scaffold	1558:1582	the BC-HA porous scaffold	1558:1582	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	11	86	theme	BC-HA	1562:1566	arg1	scaffold					1624:1631	a successful bone tissue engineering scaffold	1587:1631	a successful bone tissue engineering scaffold	1587:1631	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	0	87	theme	Composite	60:68	arg1	Scaffold					70:77	Hydroxyapatite Composite Scaffold	45:77	Hydroxyapatite Composite Scaffold for Cranial Bone Defect Repair	45:108	A Novel Porous Butyryl Chitin-Animal Derived Hydroxyapatite Composite Scaffold for Cranial Bone Defect Repair.					
37239867	9	88	theme	BC-HA	1236:1240	arg1	BC					1223:1224	BC	1223:1224	BC	1223:1224	With the aim of combining the advantages of BC and HA, a BC-HA composite scaffold, with a good pore structure and mechanical strength, was prepared by physical mixing.					
37239867	9	88	theme	BC-HA	1236:1240	arg1	scaffold					1252:1259	a BC-HA composite scaffold	1234:1259	a BC-HA composite scaffold	1234:1259	With the aim of combining the advantages of BC and HA, a BC-HA composite scaffold, with a good pore structure and mechanical strength, was prepared by physical mixing.					
37239867	3	89	theme	Butyryl	401:407	arg1	BC					417:418	BC	417:418	BC	417:418	Butyryl chitin (BC) is a derivative of chitin (CT) with improved solubility.					
37239867	3	89	theme	Butyryl	401:407	arg1	chitin					409:414	Butyryl chitin	401:414	Butyryl chitin (BC)	401:419	Butyryl chitin (BC) is a derivative of chitin (CT) with improved solubility.					
37239867	3	89	theme	Butyryl	401:407	arg1	derivative					426:435	a derivative	424:435	a derivative of chitin (CT) with improved solubility	424:475	Butyryl chitin (BC) is a derivative of chitin (CT) with improved solubility.					
37239867	11	90	theme	bone	1600:1603	arg1	engineering					1612:1622	a successful bone tissue engineering	1587:1622	a successful bone tissue engineering scaffold	1587:1631	These results prove that the BC-HA porous scaffold is a successful bone tissue engineering scaffold and has strong potential to be further developed as a substitute for bone transplantation.					
37239867	6	91	theme	film	689:692	arg1	method					694:699	the cast film method	680:699	the cast film method	680:699	BC films were prepared using the cast film method and showed strong tensile strength (47.8 ± 4.54 N) and hydrophobicity (86.4 ± 2.46°), which was favorable for mineral deposition.					
37239867	3	92	theme	improved	457:464	arg1	solubility					466:475	improved solubility	457:475	improved solubility	457:475	Butyryl chitin (BC) is a derivative of chitin (CT) with improved solubility.					
37239867	10	93	theme	perfect	1409:1415	arg1	performance					1430:1440	perfect bone-binding performance	1409:1440	perfect bone-binding performance	1409:1440	Administered into skull defects of rats, the scaffolds showed perfect bone-binding performance and effective structural support, and significantly promoted the regeneration of new bone.					
37239867	4	94	contain	has	481:483	arg1	It					478:479	It	478:479	It	478:479	It has good biocompatibility, but few studies have investigated its use in bone repair.					
37239867	4	94	contain	has	481:483	arg2	biocompatibility					490:505	good biocompatibility	485:505	good biocompatibility	485:505	It has good biocompatibility, but few studies have investigated its use in bone repair.					
37239867	9	95	theme	good	1269:1272	arg1	structure					1279:1287	a good pore structure	1267:1287	a good pore structure	1267:1287	With the aim of combining the advantages of BC and HA, a BC-HA composite scaffold, with a good pore structure and mechanical strength, was prepared by physical mixing.					
36720414	4	0	theme	them	762:765	arg1	kinetics					750:757	the adsorption kinetics	735:757	the adsorption kinetics of them	735:765	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	3	1	theme	optimal	505:511	arg1	pH					513:514	the optimal pH	501:514	the optimal pH for Cr	501:521	In mono-polluted system, the optimal pH for Cr(VI), MO (methyl orange), Cu(Ⅱ) and MB (methylene blue) were 3, 2, 6 and 12 with a saturated adsorption capacity of 87.53, 66.41, 351.03 and 286.54 mg/g, respectively.					
36720414	3	1	theme	optimal	505:511	arg1	6					589:589	6	589:589	6	589:589	In mono-polluted system, the optimal pH for Cr(VI), MO (methyl orange), Cu(Ⅱ) and MB (methylene blue) were 3, 2, 6 and 12 with a saturated adsorption capacity of 87.53, 66.41, 351.03 and 286.54 mg/g, respectively.					
36720414	7	2	theme	removal	1405:1411	arg1	mechanism					1413:1421	removal mechanism	1405:1421	removal mechanism	1405:1421	Lastly, removal mechanism was revealed in detail by FT-IR, EDS and XPS.					
36720414	0	3	theme	dyes	65:68	arg1	mechanism					188:196	mechanism	188:196	mechanism	188:196	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	0	3	theme	dyes	65:68	arg1	removal					13:19	Simultaneous removal	0:19	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	0:156	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	0	3	theme	dyes	65:68	arg1	interaction					172:182	interaction	172:182	interaction	172:182	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	1	4	theme	aqueous	249:255	arg1	system					257:262	the aqueous system	245:262	the aqueous system	245:262	Heavy metals and azo dyes caused huge harm to the aqueous system and human health.					
36720414	0	5	theme	magnetic	75:82	arg1	composite					148:156	a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	73:156	a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	73:156	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	5	6	theme	heavy	1222:1226	arg1	metals					1228:1233	heavy metals	1222:1233	heavy metals	1222:1233	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	5	7	theme	heavy	1110:1114	arg1	metals					1116:1121	heavy metals	1110:1121	heavy metals	1110:1121	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	3	8	theme	methyl	532:537	arg1	MO					528:529	MO	528:529	MO (methyl orange)	528:545	In mono-polluted system, the optimal pH for Cr(VI), MO (methyl orange), Cu(Ⅱ) and MB (methylene blue) were 3, 2, 6 and 12 with a saturated adsorption capacity of 87.53, 66.41, 351.03 and 286.54 mg/g, respectively.					
36720414	3	8	theme	methyl	532:537	arg1	orange					539:544	methyl orange	532:544	methyl orange	532:544	In mono-polluted system, the optimal pH for Cr(VI), MO (methyl orange), Cu(Ⅱ) and MB (methylene blue) were 3, 2, 6 and 12 with a saturated adsorption capacity of 87.53, 66.41, 351.03 and 286.54 mg/g, respectively.					
36720414	3	9	theme	saturated	605:613	arg1	capacity					626:633	a saturated adsorption capacity	603:633	a saturated adsorption capacity of 87.53	603:642	In mono-polluted system, the optimal pH for Cr(VI), MO (methyl orange), Cu(Ⅱ) and MB (methylene blue) were 3, 2, 6 and 12 with a saturated adsorption capacity of 87.53, 66.41, 351.03 and 286.54 mg/g, respectively.					
36720414	5	10	dep	the	1211:1213	arg1	add					1215:1217	add	1215:1217	add of heavy metals	1215:1233	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	5	11	theme	anionic	1065:1071	arg1	solution					1085:1092	anionic or cationic solution	1065:1092	anionic or cationic solution	1065:1092	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	0	12	theme	embedded	111:118	arg1	composite					148:156	a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	73:156	a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	73:156	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	3	13	theme	adsorption	615:624	arg1	capacity					626:633	a saturated adsorption capacity	603:633	a saturated adsorption capacity of 87.53	603:642	In mono-polluted system, the optimal pH for Cr(VI), MO (methyl orange), Cu(Ⅱ) and MB (methylene blue) were 3, 2, 6 and 12 with a saturated adsorption capacity of 87.53, 66.41, 351.03 and 286.54 mg/g, respectively.					
36720414	0	14	theme	chitosan/polyethyleneimine	84:109	arg1	composite					148:156	a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	73:156	a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	73:156	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	4	15	theme	entropy-increasing	888:905	arg1	process					907:913	a spontaneous, endothermic, and entropy-increasing process	856:913	a spontaneous, endothermic, and entropy-increasing process	856:913	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	4	15	theme	entropy-increasing	888:905	arg1	model					844:848	the Langmuir isotherm model	822:848	the Langmuir isotherm model	822:848	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	5	16	theme	cationic	1076:1083	arg1	solution					1085:1092	anionic or cationic solution	1065:1092	anionic or cationic solution	1065:1092	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	5	17	theme	-MO	992:994	arg1	pollutants					1009:1018	Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants	986:1018	Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants	986:1018	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	4	18	theme	endothermic	871:881	arg1	process					907:913	a spontaneous, endothermic, and entropy-increasing process	856:913	a spontaneous, endothermic, and entropy-increasing process	856:913	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	4	18	theme	endothermic	871:881	arg1	model					844:848	the Langmuir isotherm model	822:848	the Langmuir isotherm model	822:848	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	5	19	from	pH	1037:1038	arg1	adsorption					971:980	simultaneous adsorption	958:980	simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH	958:1038	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	3	20	theme	87.53	638:642	arg1	capacity					626:633	a saturated adsorption capacity	603:633	a saturated adsorption capacity of 87.53	603:642	In mono-polluted system, the optimal pH for Cr(VI), MO (methyl orange), Cu(Ⅱ) and MB (methylene blue) were 3, 2, 6 and 12 with a saturated adsorption capacity of 87.53, 66.41, 351.03 and 286.54 mg/g, respectively.					
36720414	2	21	theme	hydrophobic	329:339	arg1	composite					357:365	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	282:365	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS)	282:372	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	2	21	theme	hydrophobic	329:339	arg1	MCPS					368:371	MCPS	368:371	MCPS	368:371	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	4	22	theme	adsorption	775:784	arg1	isotherms					786:794	the adsorption isotherms	771:794	the adsorption isotherms	771:794	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	4	22	theme	adsorption	775:784	arg1	consistent					806:815	consistent	806:815	consistent	806:815	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	0	23	theme	Simultaneous	0:11	arg1	mechanism					188:196	mechanism	188:196	mechanism	188:196	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	0	23	theme	Simultaneous	0:11	arg1	removal					13:19	Simultaneous removal	0:19	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	0:156	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	0	23	theme	Simultaneous	0:11	arg1	interaction					172:182	interaction	172:182	interaction	172:182	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	1	24	theme	human	268:272	arg1	health					274:279	human health	268:279	human health	268:279	Heavy metals and azo dyes caused huge harm to the aqueous system and human health.					
36720414	4	25	theme	adsorption	739:748	arg1	kinetics					750:757	the adsorption kinetics	735:757	the adsorption kinetics of them	735:765	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	2	26	theme	embedded	320:327	arg1	composite					357:365	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	282:365	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS)	282:372	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	2	26	theme	embedded	320:327	arg1	MCPS					368:371	MCPS	368:371	MCPS	368:371	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	1	27	theme	Heavy	199:203	arg1	metals					205:210	Heavy metals	199:210	Heavy metals	199:210	Heavy metals and azo dyes caused huge harm to the aqueous system and human health.					
36720414	0	28	theme	aqueous	24:30	arg1	metals					54:59	aqueous same ionic type heavy metals	24:59	aqueous same ionic type heavy metals	24:59	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	3	29	dep	6	589:589	arg1	VI					523:524	VI	523:524	VI	523:524	In mono-polluted system, the optimal pH for Cr(VI), MO (methyl orange), Cu(Ⅱ) and MB (methylene blue) were 3, 2, 6 and 12 with a saturated adsorption capacity of 87.53, 66.41, 351.03 and 286.54 mg/g, respectively.					
36720414	0	30	theme	sodium	132:137	arg1	composite					148:156	a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	73:156	a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	73:156	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	6	31	theme	solution	1362:1369	arg1	pH					1344:1345	the pH	1340:1345	the pH of the aqueous solution to realize recyclability	1340:1394	Both Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants could be effectively adsorbed and desorbed from MCPS by changing the pH of the aqueous solution to realize recyclability.					
36720414	2	32	theme	chitosan/polyethyleneimine	293:318	arg1	composite					357:365	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	282:365	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS)	282:372	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	2	32	theme	chitosan/polyethyleneimine	293:318	arg1	MCPS					368:371	MCPS	368:371	MCPS	368:371	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	2	33	theme	ionic	438:442	arg1	metals					455:460	aqueous same ionic type heavy metals	425:460	aqueous same ionic type heavy metals	425:460	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	2	34	theme	type	444:447	arg1	metals					455:460	aqueous same ionic type heavy metals	425:460	aqueous same ionic type heavy metals	425:460	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	0	35	theme	hydrophobic	120:130	arg1	composite					148:156	a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	73:156	a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	73:156	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	3	36	theme	blue	572:575	arg1	methylene					562:570	methylene blue	562:575	methylene blue	562:575	In mono-polluted system, the optimal pH for Cr(VI), MO (methyl orange), Cu(Ⅱ) and MB (methylene blue) were 3, 2, 6 and 12 with a saturated adsorption capacity of 87.53, 66.41, 351.03 and 286.54 mg/g, respectively.					
36720414	3	36	theme	blue	572:575	arg1	MB					558:559	MB	558:559	MB (methylene blue)	558:576	In mono-polluted system, the optimal pH for Cr(VI), MO (methyl orange), Cu(Ⅱ) and MB (methylene blue) were 3, 2, 6 and 12 with a saturated adsorption capacity of 87.53, 66.41, 351.03 and 286.54 mg/g, respectively.					
36720414	2	37	theme	magnetic	284:291	arg1	composite					357:365	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	282:365	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS)	282:372	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	2	37	theme	magnetic	284:291	arg1	MCPS					368:371	MCPS	368:371	MCPS	368:371	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	5	38	contain	possessed	948:956	arg1	MCPS					943:946	MCPS	943:946	MCPS	943:946	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	5	38	contain	possessed	948:956	arg2	adsorption					971:980	simultaneous adsorption	958:980	simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH	958:1038	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	2	39	theme	same	433:436	arg1	metals					455:460	aqueous same ionic type heavy metals	425:460	aqueous same ionic type heavy metals	425:460	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	0	40	theme	ionic	37:41	arg1	metals					54:59	aqueous same ionic type heavy metals	24:59	aqueous same ionic type heavy metals	24:59	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	4	41	theme	Langmuir	826:833	arg1	process					907:913	a spontaneous, endothermic, and entropy-increasing process	856:913	a spontaneous, endothermic, and entropy-increasing process	856:913	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	4	41	theme	Langmuir	826:833	arg1	model					844:848	the Langmuir isotherm model	822:848	the Langmuir isotherm model	822:848	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	2	42	theme	aqueous	425:431	arg1	metals					455:460	aqueous same ionic type heavy metals	425:460	aqueous same ionic type heavy metals	425:460	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	5	43	theme	-MB	1005:1007	arg1	pollutants					1009:1018	Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants	986:1018	Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants	986:1018	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	5	44	theme	azo	1153:1155	arg1	dyes					1157:1160	azo dyes	1153:1160	azo dyes	1153:1160	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	1	45	theme	azo	216:218	arg1	dyes					220:223	azo dyes	216:223	azo dyes	216:223	Heavy metals and azo dyes caused huge harm to the aqueous system and human health.					
36720414	0	46	theme	same	32:35	arg1	metals					54:59	aqueous same ionic type heavy metals	24:59	aqueous same ionic type heavy metals	24:59	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	3	47	from	6	589:589	arg1	system					493:498	mono-polluted system	479:498	mono-polluted system	479:498	In mono-polluted system, the optimal pH for Cr(VI), MO (methyl orange), Cu(Ⅱ) and MB (methylene blue) were 3, 2, 6 and 12 with a saturated adsorption capacity of 87.53, 66.41, 351.03 and 286.54 mg/g, respectively.					
36720414	0	48	theme	alginate	139:146	arg1	composite					148:156	a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	73:156	a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	73:156	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	2	49	theme	heavy	449:453	arg1	metals					455:460	aqueous same ionic type heavy metals	425:460	aqueous same ionic type heavy metals	425:460	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	6	50	theme	aqueous	1354:1360	arg1	solution					1362:1369	the aqueous solution to realize recyclability	1350:1394	the aqueous solution to realize recyclability	1350:1394	Both Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants could be effectively adsorbed and desorbed from MCPS by changing the pH of the aqueous solution to realize recyclability.					
36720414	0	51	theme	heavy	48:52	arg1	metals					54:59	aqueous same ionic type heavy metals	24:59	aqueous same ionic type heavy metals	24:59	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	4	52	theme	spontaneous	858:868	arg1	process					907:913	a spontaneous, endothermic, and entropy-increasing process	856:913	a spontaneous, endothermic, and entropy-increasing process	856:913	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	4	52	theme	spontaneous	858:868	arg1	model					844:848	the Langmuir isotherm model	822:848	the Langmuir isotherm model	822:848	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	0	53	theme	type	43:46	arg1	metals					54:59	aqueous same ionic type heavy metals	24:59	aqueous same ionic type heavy metals	24:59	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	3	54	theme	mono-polluted	479:491	arg1	system					493:498	mono-polluted system	479:498	mono-polluted system	479:498	In mono-polluted system, the optimal pH for Cr(VI), MO (methyl orange), Cu(Ⅱ) and MB (methylene blue) were 3, 2, 6 and 12 with a saturated adsorption capacity of 87.53, 66.41, 351.03 and 286.54 mg/g, respectively.					
36720414	2	55	theme	azo	466:468	arg1	dyes					470:473	azo dyes	466:473	azo dyes	466:473	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	5	56	theme	metals	1116:1121	arg1	removal					1099:1105	the removal	1095:1105	the removal of heavy metals	1095:1121	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	5	57	theme	optimal	1029:1035	arg1	pH					1037:1038	their optimal pH	1023:1038	their optimal pH	1023:1038	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	5	58	theme	binary-polluted	919:933	arg1	system					935:940	binary-polluted system	919:940	binary-polluted system	919:940	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	2	59	theme	alginate	348:355	arg1	composite					357:365	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	282:365	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS)	282:372	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	2	59	theme	alginate	348:355	arg1	MCPS					368:371	MCPS	368:371	MCPS	368:371	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	5	60	dep	the	1142:1144	arg1	add					1146:1148	add	1146:1148	add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals	1146:1233	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	5	61	theme	simultaneous	958:969	arg1	adsorption					971:980	simultaneous adsorption	958:980	simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH	958:1038	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	6	62	dep	-MO	1247:1249	arg1	pollutants					1264:1273	pollutants	1264:1273	pollutants	1264:1273	Both Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants could be effectively adsorbed and desorbed from MCPS by changing the pH of the aqueous solution to realize recyclability.					
36720414	2	63	theme	sodium	341:346	arg1	composite					357:365	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	282:365	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS)	282:372	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	2	63	theme	sodium	341:346	arg1	MCPS					368:371	MCPS	368:371	MCPS	368:371	A magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite (MCPS) was designed and prepared to simultaneously remove aqueous same ionic type heavy metals and azo dyes.					
36720414	1	64	theme	huge	232:235	arg1	harm					237:240	huge harm	232:240	huge harm	232:240	Heavy metals and azo dyes caused huge harm to the aqueous system and human health.					
36720414	0	65	dep	removal	13:19	arg1	Performance					159:169	Performance	159:169	Performance	159:169	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	5	66	theme	azo	1181:1183	arg1	dyes					1185:1188	azo dyes	1181:1188	azo dyes	1181:1188	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	0	67	theme	metals	54:59	arg1	mechanism					188:196	mechanism	188:196	mechanism	188:196	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	0	67	theme	metals	54:59	arg1	removal					13:19	Simultaneous removal	0:19	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite	0:156	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	0	67	theme	metals	54:59	arg1	interaction					172:182	interaction	172:182	interaction	172:182	Simultaneous removal of aqueous same ionic type heavy metals and dyes by a magnetic chitosan/polyethyleneimine embedded hydrophobic sodium alginate composite: Performance, interaction and mechanism.					
36720414	5	68	theme	dyes	1185:1188	arg1	removal					1170:1176	the removal	1166:1176	the removal of azo dyes	1166:1188	In binary-polluted system, MCPS possessed simultaneous adsorption for Cr (Ⅵ)-MO and Cu(Ⅱ)-MB pollutants at their optimal pH, in addition, whether in anionic or cationic solution, the removal of heavy metals were promoted with the add of azo dyes but the removal of azo dyes were suppressed with the add of heavy metals.					
36720414	4	69	theme	isotherm	835:842	arg1	process					907:913	a spontaneous, endothermic, and entropy-increasing process	856:913	a spontaneous, endothermic, and entropy-increasing process	856:913	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
36720414	4	69	theme	isotherm	835:842	arg1	model					844:848	the Langmuir isotherm model	822:848	the Langmuir isotherm model	822:848	Pseudo-second-order was suitable to describe the adsorption kinetics of them and the adsorption isotherms were more consistent with the Langmuir isotherm model being a spontaneous, endothermic, and entropy-increasing process.					
37290013	3	0	theme	underlying	665:674	arg1	mechanisms					676:685	the underlying mechanisms	661:685	the underlying mechanisms	661:685	Here, we evaluated the protective effects of FL and GOS on colitis exacerbated by feeding HFrD and explored the underlying mechanisms.					
37290013	7	1	theme	Western	1065:1071	arg1	blot					1073:1076	Western blot	1065:1076	Western blot	1065:1076	Intestinal barrier integrity and inflammatory pathway expression were measured using qPCR, immunofluorescence, and Western blot methods.					
37290013	9	2	theme	junction	1418:1425	arg1	expression					1435:1444	tight junction protein expression	1412:1444	tight junction protein expression	1412:1444	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	6	3	theme	rDNA	928:931	arg1	sequencing					938:947	rDNA gene sequencing	928:947	rDNA gene sequencing	928:947	Gut microbial composition was analyzed by 16S rDNA gene sequencing.					
37290013	10	4	theme	oxidative	1562:1570	arg1	stress					1572:1577	oxidative stress	1562:1577	oxidative stress	1562:1577	Also, GOS or FL inhibited the LPS/TLR4/NF-κB signaling pathway and oxidative stress to suppress the inflammatory cascade compared with the HFrD group.					
37290013	2	5	from	protective	509:518	arg1	mice					537:540	mice	537:540	mice with HFrD	537:550	2'-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD.					
37290013	2	6	contain	have	386:389	arg2	effects					419:425	preventive and ameliorative effects	391:425	preventive and ameliorative effects on colitis	391:436	2'-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD.					
37290013	2	6	contain	have	386:389	arg1	FL					315:316	FL	315:316	FL	315:316	2'-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD.					
37290013	2	6	contain	have	386:389	arg1	galactooligosaccharide					323:344	galactooligosaccharide	323:344	galactooligosaccharide (GOS)	323:350	2'-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD.					
37290013	2	6	contain	have	386:389	arg1	2'-Fucosyllactose					296:312	2'-Fucosyllactose	296:312	2'-Fucosyllactose (FL)	296:317	2'-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD.					
37290013	2	6	contain	have	386:389	arg1	GOS					347:349	GOS	347:349	GOS	347:349	2'-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD.					
37290013	0	7	theme	DSS-Induced	126:136	arg1	Colitis					138:144	DSS-Induced Colitis	126:144	DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice	126:187	Galactooligosaccharide or 2'-Fucosyllactose Modulates Gut Microbiota and Inhibits LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice.					
37290013	9	8	theme	tight	1412:1416	arg1	expression					1435:1444	tight junction protein expression	1412:1444	tight junction protein expression	1412:1444	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	4	9	theme	C57BL/6J	739:746	arg1	mice					753:756	four randomized C57BL/6J male mice	723:756	four randomized C57BL/6J male mice (n = 8 mice/group)	723:775	DSS-induced colitis was studied in four randomized C57BL/6J male mice (n = 8 mice/group).					
37290013	8	10	theme	short-chain	1260:1270	arg1	acids					1278:1282	short-chain fatty acids	1260:1282	short-chain fatty acids (SCFAs)	1260:1290	Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively.					
37290013	8	10	theme	short-chain	1260:1270	arg1	SCFAs					1285:1289	SCFAs	1285:1289	SCFAs	1285:1289	Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively.					
37290013	4	11	theme	randomized	728:737	arg1	mice					753:756	four randomized C57BL/6J male mice	723:756	four randomized C57BL/6J male mice (n = 8 mice/group)	723:775	DSS-induced colitis was studied in four randomized C57BL/6J male mice (n = 8 mice/group).					
37290013	5	12	theme	FL	854:855	arg1	treatment					857:865	FL treatment	854:865	FL treatment	854:865	Among them, three groups were fed with HFrD, and two received either GOS or FL treatment, respectively.					
37290013	9	13	theme	cells	1385:1389	arg1	loss					1370:1373	the loss	1366:1373	the loss of goblet cells	1366:1389	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	9	13	theme	cells	1385:1389	arg1	reduction					1399:1407	the reduction	1395:1407	the reduction of tight junction protein expression	1395:1444	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	3	14	from	effects	587:593	arg1	colitis					612:618	colitis	612:618	colitis exacerbated by feeding HFrD	612:646	Here, we evaluated the protective effects of FL and GOS on colitis exacerbated by feeding HFrD and explored the underlying mechanisms.					
37290013	1	15	theme	high-fructose	192:204	arg1	HFrD					212:215	HFrD	212:215	HFrD	212:215	A high-fructose diet (HFrD) has been reported to exacerbate dextran sulfate sodium (DSS)-induced colitis.					
37290013	1	15	theme	high-fructose	192:204	arg1	diet					206:209	A high-fructose diet	190:209	A high-fructose diet (HFrD)	190:216	A high-fructose diet (HFrD) has been reported to exacerbate dextran sulfate sodium (DSS)-induced colitis.					
37290013	9	16	theme	GOS	1337:1339	arg1	treatment					1347:1355	GOS or FL treatment	1337:1355	treatment	1347:1355	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	7	17	theme	immunofluorescence	1041:1058	arg1	methods					1078:1084	qPCR, immunofluorescence, and Western blot methods	1035:1084	qPCR, immunofluorescence, and Western blot methods	1035:1084	Intestinal barrier integrity and inflammatory pathway expression were measured using qPCR, immunofluorescence, and Western blot methods.					
37290013	4	18	theme	male	748:751	arg1	mice					753:756	four randomized C57BL/6J male mice	723:756	four randomized C57BL/6J male mice (n = 8 mice/group)	723:775	DSS-induced colitis was studied in four randomized C57BL/6J male mice (n = 8 mice/group).					
37290013	10	19	theme	signaling	1540:1548	arg1	pathway					1550:1556	the LPS/TLR4/NF-κB signaling pathway	1521:1556	the LPS/TLR4/NF-κB signaling pathway	1521:1556	Also, GOS or FL inhibited the LPS/TLR4/NF-κB signaling pathway and oxidative stress to suppress the inflammatory cascade compared with the HFrD group.					
37290013	4	20	dep	=	761:761	arg1	mice/group					765:774	8 mice/group	763:774	8 mice/group	763:774	DSS-induced colitis was studied in four randomized C57BL/6J male mice (n = 8 mice/group).					
37290013	11	21	theme	HFrD-exacerbated	1705:1720	arg1	colitis					1722:1728	HFrD-exacerbated colitis	1705:1728	HFrD-exacerbated colitis	1705:1728	These findings suggest that GOS or FL intake can alleviate HFrD-exacerbated colitis, with no significant difference observed between GOS and FL treatments.					
37290013	9	22	theme	intestinal	1465:1474	arg1	integrity					1484:1492	intestinal barrier integrity	1465:1492	intestinal barrier integrity	1465:1492	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	1	23	theme	-induced	278:285	arg1	colitis					287:293	dextran sulfate sodium (DSS)-induced colitis	250:293	dextran sulfate sodium (DSS)-induced colitis	250:293	A high-fructose diet (HFrD) has been reported to exacerbate dextran sulfate sodium (DSS)-induced colitis.					
37290013	7	24	theme	qPCR	1035:1038	arg1	methods					1078:1084	qPCR, immunofluorescence, and Western blot methods	1035:1084	qPCR, immunofluorescence, and Western blot methods	1035:1084	Intestinal barrier integrity and inflammatory pathway expression were measured using qPCR, immunofluorescence, and Western blot methods.					
37290013	10	25	theme	inflammatory	1595:1606	arg1	cascade					1608:1614	the inflammatory cascade	1591:1614	the inflammatory cascade	1591:1614	Also, GOS or FL inhibited the LPS/TLR4/NF-κB signaling pathway and oxidative stress to suppress the inflammatory cascade compared with the HFrD group.					
37290013	9	26	theme	barrier	1476:1482	arg1	integrity					1484:1492	intestinal barrier integrity	1465:1492	intestinal barrier integrity	1465:1492	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	11	27	dep	GOS	1779:1781	arg1	treatments					1790:1799	treatments	1790:1799	treatments	1790:1799	These findings suggest that GOS or FL intake can alleviate HFrD-exacerbated colitis, with no significant difference observed between GOS and FL treatments.					
37290013	7	28	theme	blot	1073:1076	arg1	methods					1078:1084	qPCR, immunofluorescence, and Western blot methods	1035:1084	qPCR, immunofluorescence, and Western blot methods	1035:1084	Intestinal barrier integrity and inflammatory pathway expression were measured using qPCR, immunofluorescence, and Western blot methods.					
37290013	11	29	theme	FL	1681:1682	arg1	intake					1684:1689	FL intake	1681:1689	FL intake	1681:1689	These findings suggest that GOS or FL intake can alleviate HFrD-exacerbated colitis, with no significant difference observed between GOS and FL treatments.					
37290013	11	30	theme	significant	1739:1749	arg1	difference					1751:1760	no significant difference	1736:1760	no significant difference observed between GOS and FL treatments	1736:1799	These findings suggest that GOS or FL intake can alleviate HFrD-exacerbated colitis, with no significant difference observed between GOS and FL treatments.					
37290013	8	31	theme	FL	1122:1123	arg1	treatment					1125:1133	FL treatment	1122:1133	FL treatment	1122:1133	Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively.					
37290013	6	32	theme	microbial	886:894	arg1	composition					896:906	Gut microbial composition	882:906	Gut microbial composition	882:906	Gut microbial composition was analyzed by 16S rDNA gene sequencing.					
37290013	9	33	theme	protein	1427:1433	arg1	expression					1435:1444	tight junction protein expression	1412:1444	tight junction protein expression	1412:1444	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	10	34	theme	HFrD	1634:1637	arg1	group					1639:1643	the HFrD group	1630:1643	the HFrD group	1630:1643	Also, GOS or FL inhibited the LPS/TLR4/NF-κB signaling pathway and oxidative stress to suppress the inflammatory cascade compared with the HFrD group.					
37290013	2	35	theme	ameliorative	406:417	arg1	effects					419:425	preventive and ameliorative effects	391:425	preventive and ameliorative effects on colitis	391:436	2'-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD.					
37290013	10	36	theme	LPS/TLR4/NF-κB	1525:1538	arg1	pathway					1550:1556	the LPS/TLR4/NF-κB signaling pathway	1521:1556	the LPS/TLR4/NF-κB signaling pathway	1521:1556	Also, GOS or FL inhibited the LPS/TLR4/NF-κB signaling pathway and oxidative stress to suppress the inflammatory cascade compared with the HFrD group.					
37290013	6	37	theme	Gut	882:884	arg1	composition					896:906	Gut microbial composition	882:906	Gut microbial composition	882:906	Gut microbial composition was analyzed by 16S rDNA gene sequencing.					
37290013	7	38	theme	pathway	996:1002	arg1	expression					1004:1013	inflammatory pathway expression	983:1013	inflammatory pathway expression	983:1013	Intestinal barrier integrity and inflammatory pathway expression were measured using qPCR, immunofluorescence, and Western blot methods.					
37290013	8	39	theme	microbiota	1172:1181	arg1	α-diversity					1149:1159	the α-diversity	1145:1159	the α-diversity of the gut microbiota	1145:1181	Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively.					
37290013	0	40	theme	Gut	54:56	arg1	Microbiota					58:67	Gut Microbiota	54:67	Gut Microbiota	54:67	Galactooligosaccharide or 2'-Fucosyllactose Modulates Gut Microbiota and Inhibits LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice.					
37290013	0	41	theme	High-Fructose	162:174	arg1	Diet					176:179	a High-Fructose Diet	160:179	a High-Fructose Diet in Mice	160:187	Galactooligosaccharide or 2'-Fucosyllactose Modulates Gut Microbiota and Inhibits LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice.					
37290013	9	42	theme	goblet	1378:1383	arg1	cells					1385:1389	goblet cells	1378:1389	goblet cells	1378:1389	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	9	43	theme	HFrD	1325:1328	arg1	group					1330:1334	the HFrD group	1321:1334	the HFrD group	1321:1334	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	2	44	theme	preventive	391:400	arg1	effects					419:425	preventive and ameliorative effects	391:425	preventive and ameliorative effects on colitis	391:436	2'-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD.					
37290013	8	45	theme	HFrD	1103:1106	arg1	group					1108:1112	the HFrD group	1099:1112	the HFrD group	1099:1112	Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively.					
37290013	8	46	theme	fatty	1272:1276	arg1	acids					1278:1282	short-chain fatty acids	1260:1282	short-chain fatty acids (SCFAs)	1260:1290	Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively.					
37290013	8	46	theme	fatty	1272:1276	arg1	SCFAs					1285:1289	SCFAs	1285:1289	SCFAs	1285:1289	Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively.					
37290013	3	47	theme	GOS	605:607	arg1	effects					587:593	the protective effects	572:593	the protective effects of FL and GOS on colitis exacerbated by feeding HFrD	572:646	Here, we evaluated the protective effects of FL and GOS on colitis exacerbated by feeding HFrD and explored the underlying mechanisms.					
37290013	7	48	theme	Intestinal	950:959	arg1	integrity					969:977	Intestinal barrier integrity	950:977	Intestinal barrier integrity	950:977	Intestinal barrier integrity and inflammatory pathway expression were measured using qPCR, immunofluorescence, and Western blot methods.					
37290013	8	49	theme	Akkermansia	1218:1228	arg1	abundance					1205:1213	the relative abundance	1192:1213	the relative abundance of Akkermansia	1192:1228	Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively.					
37290013	2	50	theme	limited	445:451	arg1	research					453:460	limited research	445:460	limited research	445:460	2'-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD.					
37290013	7	51	theme	inflammatory	983:994	arg1	expression					1004:1013	inflammatory pathway expression	983:1013	inflammatory pathway expression	983:1013	Intestinal barrier integrity and inflammatory pathway expression were measured using qPCR, immunofluorescence, and Western blot methods.					
37290013	9	52	theme	expression	1435:1444	arg1	loss					1370:1373	the loss	1366:1373	the loss of goblet cells	1366:1389	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	9	52	theme	expression	1435:1444	arg1	reduction					1399:1407	the reduction	1395:1407	the reduction of tight junction protein expression	1395:1444	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	7	53	theme	barrier	961:967	arg1	integrity					969:977	Intestinal barrier integrity	950:977	Intestinal barrier integrity	950:977	Intestinal barrier integrity and inflammatory pathway expression were measured using qPCR, immunofluorescence, and Western blot methods.					
37290013	0	54	from	Diet	176:179	arg1	Mice					184:187	Mice	184:187	Mice	184:187	Galactooligosaccharide or 2'-Fucosyllactose Modulates Gut Microbiota and Inhibits LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice.					
37290013	8	55	theme	acids	1278:1282	arg1	content					1249:1255	the content	1245:1255	the content of short-chain fatty acids (SCFAs)	1245:1290	Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively.					
37290013	3	56	theme	exacerbated	620:630	arg1	colitis					612:618	colitis	612:618	colitis exacerbated by feeding HFrD	612:646	Here, we evaluated the protective effects of FL and GOS on colitis exacerbated by feeding HFrD and explored the underlying mechanisms.					
37290013	3	57	theme	FL	598:599	arg1	effects					587:593	the protective effects	572:593	the protective effects of FL and GOS on colitis exacerbated by feeding HFrD	572:646	Here, we evaluated the protective effects of FL and GOS on colitis exacerbated by feeding HFrD and explored the underlying mechanisms.					
37290013	4	58	theme	DSS-induced	688:698	arg1	colitis					700:706	DSS-induced colitis	688:706	DSS-induced colitis	688:706	DSS-induced colitis was studied in four randomized C57BL/6J male mice (n = 8 mice/group).					
37290013	2	59	with	mice	537:540	arg1	HFrD					547:550	HFrD	547:550	HFrD	547:550	2'-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD.					
37290013	0	60	theme	Signaling	97:105	arg1	Pathway					107:113	LPS/TLR4/NF-κB Signaling Pathway	82:113	LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice	82:187	Galactooligosaccharide or 2'-Fucosyllactose Modulates Gut Microbiota and Inhibits LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice.					
37290013	8	61	theme	relative	1196:1203	arg1	abundance					1205:1213	the relative abundance	1192:1213	the relative abundance of Akkermansia	1192:1228	Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively.					
37290013	3	62	theme	protective	576:585	arg1	effects					587:593	the protective effects	572:593	the protective effects of FL and GOS on colitis exacerbated by feeding HFrD	572:646	Here, we evaluated the protective effects of FL and GOS on colitis exacerbated by feeding HFrD and explored the underlying mechanisms.					
37290013	4	63	dep	mice	753:756	arg1	n					759:759	n	759:759	n = 8 mice/group	759:774	DSS-induced colitis was studied in four randomized C57BL/6J male mice (n = 8 mice/group).					
37290013	9	64	theme	FL	1344:1345	arg1	treatment					1347:1355	GOS or FL treatment	1337:1355	treatment	1347:1355	Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity.					
37290013	2	65	from	mice	537:540	arg1	protective					509:518	protective	509:518	protective	509:518	2'-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD.					
37290013	0	66	theme	LPS/TLR4/NF-κB	82:95	arg1	Pathway					107:113	LPS/TLR4/NF-κB Signaling Pathway	82:113	LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice	82:187	Galactooligosaccharide or 2'-Fucosyllactose Modulates Gut Microbiota and Inhibits LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice.					
37290013	4	67	theme	=	761:761	arg1	n					759:759	n	759:759	n = 8 mice/group	759:774	DSS-induced colitis was studied in four randomized C57BL/6J male mice (n = 8 mice/group).					
37290013	2	68	from	effects	419:425	arg1	colitis					430:436	colitis	430:436	colitis	430:436	2'-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD.					
37290013	8	69	theme	gut	1168:1170	arg1	microbiota					1172:1181	the gut microbiota	1164:1181	the gut microbiota	1164:1181	Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively.					
37290013	6	70	theme	gene	933:936	arg1	sequencing					938:947	rDNA gene sequencing	928:947	rDNA gene sequencing	928:947	Gut microbial composition was analyzed by 16S rDNA gene sequencing.					
37376238	10	0	theme	water	1504:1508	arg1	content					1510:1516	water content	1504:1516	water content	1504:1516	It was concluded that alginate and CaCl2 concentrations play the most important role regarding the gelation time, homogeneity, water content, and swelling ratio of the hydrogel.					
37376238	2	1	used	used	224:227	arg2	polymers					185:192	Many natural polymers	172:192	Many natural polymers	172:192	Many natural polymers, such as polysaccharides, are used for the preparation of hydrogels.					
37376238	2	1	used	used	224:227	arg2	polysaccharides					203:217	polysaccharides	203:217	polysaccharides	203:217	Many natural polymers, such as polysaccharides, are used for the preparation of hydrogels.					
37376238	9	2	theme	water	1288:1292	arg1	content					1294:1300	water content	1288:1300	water content	1288:1300	Fourier transform infrared spectroscopy was used for the structural characterization of the prepared hydrogels, while the water content and swelling ratio of hydrogels were determined using gravimetric methods.					
37376238	8	3	theme	water-retaining	1049:1063	arg1	capacity					1065:1072	a water-retaining capacity	1047:1072	a water-retaining capacity of about 76%	1047:1085	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
37376238	4	4	theme	alginate	420:427	arg1	hydrogel					429:436	alginate hydrogel	420:436	alginate hydrogel	420:436	Since the properties of alginate hydrogel and its application depend on numerous factors, this study aimed to optimize the gel composition to enable the growth of inoculated cyanobacterial crusts for suppressing the desertification process.					
37376238	5	5	theme	surface	797:803	arg1	methodology					805:815	the response surface methodology	784:815	the response surface methodology	784:815	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity was analyzed using the response surface methodology.					
37376238	10	6	dep	alginate	1399:1406	arg1	concentrations					1418:1431	concentrations	1418:1431	concentrations	1418:1431	It was concluded that alginate and CaCl2 concentrations play the most important role regarding the gelation time, homogeneity, water content, and swelling ratio of the hydrogel.					
37376238	5	7	dep	concentration	703:715	arg1	%					725:725	0.4-4.6%	718:725	0.4-4.6%	718:725	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity was analyzed using the response surface methodology.					
37376238	5	7	dep	concentration	703:715	arg1	m/v					728:730	m/v	728:730	m/v	728:730	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity was analyzed using the response surface methodology.					
37376238	10	8	theme	hydrogel	1545:1552	arg1	ratio					1532:1536	swelling ratio	1523:1536	swelling ratio of the hydrogel	1523:1552	It was concluded that alginate and CaCl2 concentrations play the most important role regarding the gelation time, homogeneity, water content, and swelling ratio of the hydrogel.					
37376238	10	8	theme	hydrogel	1545:1552	arg1	homogeneity					1491:1501	homogeneity	1491:1501	homogeneity	1491:1501	It was concluded that alginate and CaCl2 concentrations play the most important role regarding the gelation time, homogeneity, water content, and swelling ratio of the hydrogel.					
37376238	10	8	theme	hydrogel	1545:1552	arg1	content					1510:1516	water content	1504:1516	water content	1504:1516	It was concluded that alginate and CaCl2 concentrations play the most important role regarding the gelation time, homogeneity, water content, and swelling ratio of the hydrogel.					
37376238	10	8	theme	hydrogel	1545:1552	arg1	time					1485:1488	the gelation time	1472:1488	the gelation time	1472:1488	It was concluded that alginate and CaCl2 concentrations play the most important role regarding the gelation time, homogeneity, water content, and swelling ratio of the hydrogel.					
37376238	2	9	theme	natural	177:183	arg1	polymers					185:192	Many natural polymers	172:192	Many natural polymers	172:192	Many natural polymers, such as polysaccharides, are used for the preparation of hydrogels.					
37376238	2	9	theme	natural	177:183	arg1	polysaccharides					203:217	polysaccharides	203:217	polysaccharides	203:217	Many natural polymers, such as polysaccharides, are used for the preparation of hydrogels.					
37376238	9	10	used	used	1210:1213	arg2	Fourier					1166:1172	Fourier	1166:1172	Fourier transform infrared spectroscopy	1166:1204	Fourier transform infrared spectroscopy was used for the structural characterization of the prepared hydrogels, while the water content and swelling ratio of hydrogels were determined using gravimetric methods.					
37376238	4	11	theme	desertification	612:626	arg1	process					628:634	the desertification process	608:634	the desertification process	608:634	Since the properties of alginate hydrogel and its application depend on numerous factors, this study aimed to optimize the gel composition to enable the growth of inoculated cyanobacterial crusts for suppressing the desertification process.					
37376238	8	12	with	hydrogel	1033:1040	arg1	capacity					1065:1072	a water-retaining capacity	1047:1072	a water-retaining capacity of about 76%	1047:1085	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
37376238	2	13	theme	Many	172:175	arg1	polymers					185:192	Many natural polymers	172:192	Many natural polymers	172:192	Many natural polymers, such as polysaccharides, are used for the preparation of hydrogels.					
37376238	2	13	theme	Many	172:175	arg1	polysaccharides					203:217	polysaccharides	203:217	polysaccharides	203:217	Many natural polymers, such as polysaccharides, are used for the preparation of hydrogels.					
37376238	5	14	theme	alginate	654:661	arg1	concentration					663:675	alginate concentration	654:675	alginate concentration (0.1-2.9%, m/v)	654:691	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity was analyzed using the response surface methodology.					
37376238	4	15	theme	hydrogel	429:436	arg1	properties					406:415	the properties	402:415	the properties of alginate hydrogel and its application	402:456	Since the properties of alginate hydrogel and its application depend on numerous factors, this study aimed to optimize the gel composition to enable the growth of inoculated cyanobacterial crusts for suppressing the desertification process.					
37376238	10	16	theme	important	1447:1455	arg1	role					1457:1460	the most important role	1438:1460	the most important role	1438:1460	It was concluded that alginate and CaCl2 concentrations play the most important role regarding the gelation time, homogeneity, water content, and swelling ratio of the hydrogel.					
37376238	5	17	theme	water-retaining	740:754	arg1	capacity					756:763	the water-retaining capacity	736:763	the water-retaining capacity	736:763	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity was analyzed using the response surface methodology.					
37376238	5	18	theme	concentration	663:675	arg1	influence					641:649	The influence	637:649	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity	637:763	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity was analyzed using the response surface methodology.					
37376238	7	19	theme	system	955:960	arg1	capacity					927:934	The water-retaining capacity	907:934	The water-retaining capacity	907:934	The water-retaining capacity was defined as the system response maximized in optimization studies.					
37376238	7	19	theme	system	955:960	arg1	response					962:969	the system response	951:969	the system response maximized in optimization studies	951:1003	The water-retaining capacity was defined as the system response maximized in optimization studies.					
37376238	10	20	theme	gelation	1476:1483	arg1	time					1485:1488	the gelation time	1472:1488	the gelation time	1472:1488	It was concluded that alginate and CaCl2 concentrations play the most important role regarding the gelation time, homogeneity, water content, and swelling ratio of the hydrogel.					
37376238	8	21	theme	%	1142:1142	arg1	solution					1156:1163	0.9% (m/v) CaCl2 solution	1139:1163	0.9% (m/v) CaCl2 solution	1139:1163	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
37376238	9	22	theme	hydrogels	1324:1332	arg1	content					1294:1300	water content	1288:1300	water content	1288:1300	Fourier transform infrared spectroscopy was used for the structural characterization of the prepared hydrogels, while the water content and swelling ratio of hydrogels were determined using gravimetric methods.					
37376238	9	22	theme	hydrogels	1324:1332	arg1	ratio					1315:1319	swelling ratio	1306:1319	swelling ratio	1306:1319	Fourier transform infrared spectroscopy was used for the structural characterization of the prepared hydrogels, while the water content and swelling ratio of hydrogels were determined using gravimetric methods.					
37376238	9	23	theme	swelling	1306:1313	arg1	ratio					1315:1319	swelling ratio	1306:1319	swelling ratio	1306:1319	Fourier transform infrared spectroscopy was used for the structural characterization of the prepared hydrogels, while the water content and swelling ratio of hydrogels were determined using gravimetric methods.					
37376238	4	24	theme	cyanobacterial	570:583	arg1	crusts					585:590	inoculated cyanobacterial crusts	559:590	inoculated cyanobacterial crusts	559:590	Since the properties of alginate hydrogel and its application depend on numerous factors, this study aimed to optimize the gel composition to enable the growth of inoculated cyanobacterial crusts for suppressing the desertification process.					
37376238	8	25	theme	%	1085:1085	arg1	capacity					1065:1072	a water-retaining capacity	1047:1072	a water-retaining capacity of about 76%	1047:1085	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
37376238	6	26	theme	different	869:877	arg1	compositions					879:890	different compositions	869:890	different compositions	869:890	According to the design matrix, 13 formulations of different compositions were prepared.					
37376238	4	27	theme	inoculated	559:568	arg1	crusts					585:590	inoculated cyanobacterial crusts	559:590	inoculated cyanobacterial crusts	559:590	Since the properties of alginate hydrogel and its application depend on numerous factors, this study aimed to optimize the gel composition to enable the growth of inoculated cyanobacterial crusts for suppressing the desertification process.					
37376238	8	28	theme	2.7	1106:1108	arg1	%					1109:1109	%	1109:1109	%	1109:1109	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
37376238	8	29	theme	optimal	1010:1016	arg1	composition					1018:1028	The optimal composition	1006:1028	The optimal composition of hydrogel with a water-retaining capacity of about 76%	1006:1085	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
37376238	1	30	theme	attractive	110:119	arg1	Hydrogels					91:99	Hydrogels	91:99	Hydrogels	91:99	Hydrogels are very attractive materials due to their multifunctional properties.					
37376238	1	30	theme	attractive	110:119	arg1	materials					121:129	very attractive materials	105:129	very attractive materials due to their multifunctional properties	105:169	Hydrogels are very attractive materials due to their multifunctional properties.					
37376238	7	31	theme	optimization	984:995	arg1	studies					997:1003	optimization studies	984:1003	optimization studies	984:1003	The water-retaining capacity was defined as the system response maximized in optimization studies.					
37376238	9	32	theme	structural	1223:1232	arg1	characterization					1234:1249	the structural characterization	1219:1249	the structural characterization of the prepared hydrogels	1219:1275	Fourier transform infrared spectroscopy was used for the structural characterization of the prepared hydrogels, while the water content and swelling ratio of hydrogels were determined using gravimetric methods.					
37376238	3	33	theme	used	295:298	arg1	alginate					318:325	alginate	318:325	alginate	318:325	The most important and commonly used polysaccharide is alginate because of its biodegradability, biocompatibility, and non-toxicity.					
37376238	3	33	theme	used	295:298	arg1	polysaccharide					300:313	The most important and commonly used polysaccharide	263:313	The most important and commonly used polysaccharide	263:313	The most important and commonly used polysaccharide is alginate because of its biodegradability, biocompatibility, and non-toxicity.					
37376238	6	34	theme	compositions	879:890	arg1	formulations					853:864	13 formulations	850:864	13 formulations of different compositions	850:890	According to the design matrix, 13 formulations of different compositions were prepared.					
37376238	10	35	gly	homogeneity	1491:1501	arg1	hydrogel					1545:1552	the hydrogel	1541:1552	the hydrogel	1541:1552	It was concluded that alginate and CaCl2 concentrations play the most important role regarding the gelation time, homogeneity, water content, and swelling ratio of the hydrogel.					
37376238	4	36	theme	crusts	585:590	arg1	growth					549:554	the growth	545:554	the growth of inoculated cyanobacterial crusts	545:590	Since the properties of alginate hydrogel and its application depend on numerous factors, this study aimed to optimize the gel composition to enable the growth of inoculated cyanobacterial crusts for suppressing the desertification process.					
37376238	3	37	theme	important	272:280	arg1	alginate					318:325	alginate	318:325	alginate	318:325	The most important and commonly used polysaccharide is alginate because of its biodegradability, biocompatibility, and non-toxicity.					
37376238	3	37	theme	important	272:280	arg1	polysaccharide					300:313	The most important and commonly used polysaccharide	263:313	The most important and commonly used polysaccharide	263:313	The most important and commonly used polysaccharide is alginate because of its biodegradability, biocompatibility, and non-toxicity.					
37376238	4	38	theme	numerous	468:475	arg1	factors					477:483	numerous factors	468:483	numerous factors	468:483	Since the properties of alginate hydrogel and its application depend on numerous factors, this study aimed to optimize the gel composition to enable the growth of inoculated cyanobacterial crusts for suppressing the desertification process.					
37376238	9	39	dep	Fourier	1166:1172	arg1	transform					1174:1182	transform	1174:1182	transform infrared spectroscopy	1174:1204	Fourier transform infrared spectroscopy was used for the structural characterization of the prepared hydrogels, while the water content and swelling ratio of hydrogels were determined using gravimetric methods.					
37376238	5	40	theme	response	788:795	arg1	methodology					805:815	the response surface methodology	784:815	the response surface methodology	784:815	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity was analyzed using the response surface methodology.					
37376238	9	41	theme	gravimetric	1356:1366	arg1	methods					1368:1374	gravimetric methods	1356:1374	gravimetric methods	1356:1374	Fourier transform infrared spectroscopy was used for the structural characterization of the prepared hydrogels, while the water content and swelling ratio of hydrogels were determined using gravimetric methods.					
37376238	0	42	theme	Alginate	36:43	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Preparation and Characterization of Alginate Hydrogels with High Water-Retaining Capacity.					
37376238	0	42	theme	Alginate	36:43	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Characterization of Alginate Hydrogels with High Water-Retaining Capacity.					
37376238	8	43	theme	CaCl2	1150:1154	arg1	solution					1156:1163	0.9% (m/v) CaCl2 solution	1139:1163	0.9% (m/v) CaCl2 solution	1139:1163	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
37376238	0	44	theme	High	60:63	arg1	Capacity					81:88	High Water-Retaining Capacity	60:88	High Water-Retaining Capacity	60:88	Preparation and Characterization of Alginate Hydrogels with High Water-Retaining Capacity.					
37376238	10	45	theme	swelling	1523:1530	arg1	ratio					1532:1536	swelling ratio	1523:1536	swelling ratio of the hydrogel	1523:1552	It was concluded that alginate and CaCl2 concentrations play the most important role regarding the gelation time, homogeneity, water content, and swelling ratio of the hydrogel.					
37376238	9	46	dep	content	1294:1300	arg1	the					1284:1286	the	1284:1286	the	1284:1286	Fourier transform infrared spectroscopy was used for the structural characterization of the prepared hydrogels, while the water content and swelling ratio of hydrogels were determined using gravimetric methods.					
37376238	1	47	theme	due	131:133	arg1	Hydrogels					91:99	Hydrogels	91:99	Hydrogels	91:99	Hydrogels are very attractive materials due to their multifunctional properties.					
37376238	1	47	theme	due	131:133	arg1	materials					121:129	very attractive materials	105:129	very attractive materials due to their multifunctional properties	105:169	Hydrogels are very attractive materials due to their multifunctional properties.					
37376238	5	48	dep	concentration	663:675	arg1	m/v					688:690	m/v	688:690	m/v	688:690	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity was analyzed using the response surface methodology.					
37376238	5	48	dep	concentration	663:675	arg1	%					685:685	0.1-2.9%	678:685	0.1-2.9%	678:685	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity was analyzed using the response surface methodology.					
37376238	5	49	theme	CaCl2	697:701	arg1	concentration					703:715	CaCl2 concentration	697:715	CaCl2 concentration (0.4-4.6%, m/v)	697:731	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity was analyzed using the response surface methodology.					
37376238	4	50	theme	application	446:456	arg1	properties					406:415	the properties	402:415	the properties of alginate hydrogel and its application	402:456	Since the properties of alginate hydrogel and its application depend on numerous factors, this study aimed to optimize the gel composition to enable the growth of inoculated cyanobacterial crusts for suppressing the desertification process.					
37376238	8	51	dep	%	1142:1142	arg1	m/v					1145:1147	m/v	1145:1147	m/v	1145:1147	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
37376238	9	52	dep	transform	1174:1182	arg1	infrared					1184:1191	infrared	1184:1191	transform infrared spectroscopy	1174:1204	Fourier transform infrared spectroscopy was used for the structural characterization of the prepared hydrogels, while the water content and swelling ratio of hydrogels were determined using gravimetric methods.					
37376238	5	53	theme	concentration	703:715	arg1	influence					641:649	The influence	637:649	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity	637:763	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity was analyzed using the response surface methodology.					
37376238	8	54	theme	hydrogel	1033:1040	arg1	composition					1018:1028	The optimal composition	1006:1028	The optimal composition of hydrogel with a water-retaining capacity of about 76%	1006:1085	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
37376238	8	55	theme	alginate	1117:1124	arg1	solution					1126:1133	2.7% (m/v) alginate solution	1106:1133	2.7% (m/v) alginate solution	1106:1133	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
37376238	9	56	theme	hydrogels	1267:1275	arg1	characterization					1234:1249	the structural characterization	1219:1249	the structural characterization of the prepared hydrogels	1219:1275	Fourier transform infrared spectroscopy was used for the structural characterization of the prepared hydrogels, while the water content and swelling ratio of hydrogels were determined using gravimetric methods.					
37376238	8	57	dep	%	1109:1109	arg1	m/v					1112:1114	m/v	1112:1114	m/v	1112:1114	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
37376238	1	58	theme	multifunctional	144:158	arg1	properties					160:169	their multifunctional properties	138:169	their multifunctional properties	138:169	Hydrogels are very attractive materials due to their multifunctional properties.					
37376238	7	59	theme	water-retaining	911:925	arg1	capacity					927:934	The water-retaining capacity	907:934	The water-retaining capacity	907:934	The water-retaining capacity was defined as the system response maximized in optimization studies.					
37376238	7	59	theme	water-retaining	911:925	arg1	response					962:969	the system response	951:969	the system response maximized in optimization studies	951:1003	The water-retaining capacity was defined as the system response maximized in optimization studies.					
37376238	8	60	theme	0.9	1139:1141	arg1	%					1142:1142	%	1142:1142	%	1142:1142	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
37376238	6	61	theme	design	835:840	arg1	matrix					842:847	the design matrix	831:847	the design matrix	831:847	According to the design matrix, 13 formulations of different compositions were prepared.					
37376238	5	62	from	influence	641:649	arg1	capacity					756:763	the water-retaining capacity	736:763	the water-retaining capacity	736:763	The influence of alginate concentration (0.1-2.9%, m/v) and CaCl2 concentration (0.4-4.6%, m/v) on the water-retaining capacity was analyzed using the response surface methodology.					
37376238	9	63	theme	prepared	1258:1265	arg1	hydrogels					1267:1275	the prepared hydrogels	1254:1275	the prepared hydrogels	1254:1275	Fourier transform infrared spectroscopy was used for the structural characterization of the prepared hydrogels, while the water content and swelling ratio of hydrogels were determined using gravimetric methods.					
37376238	0	64	theme	Water-Retaining	65:79	arg1	Capacity					81:88	High Water-Retaining Capacity	60:88	High Water-Retaining Capacity	60:88	Preparation and Characterization of Alginate Hydrogels with High Water-Retaining Capacity.					
37376238	4	65	theme	gel	519:521	arg1	composition					523:533	the gel composition	515:533	the gel composition to enable the growth of inoculated cyanobacterial crusts for suppressing the desertification process	515:634	Since the properties of alginate hydrogel and its application depend on numerous factors, this study aimed to optimize the gel composition to enable the growth of inoculated cyanobacterial crusts for suppressing the desertification process.					
37376238	2	66	theme	hydrogels	252:260	arg1	preparation					237:247	the preparation	233:247	the preparation of hydrogels	233:260	Many natural polymers, such as polysaccharides, are used for the preparation of hydrogels.					
37376238	8	67	theme	%	1109:1109	arg1	solution					1126:1133	2.7% (m/v) alginate solution	1106:1133	2.7% (m/v) alginate solution	1106:1133	The optimal composition of hydrogel with a water-retaining capacity of about 76% was obtained using 2.7% (m/v) alginate solution and 0.9% (m/v) CaCl2 solution.					
35283424	7	0	theme	immune	946:951	arg1	system					953:958	the immune system	942:958	the immune system of stress-induced mice	942:981	Although the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice remains unclear, the postbiotics mixture-induced NK cell activation might have affected gut microbiome modulation.					
35283424	3	1	from	modulation	493:502	arg1	mice					522:525	stress-induced mice	507:525	stress-induced mice	507:525	In this study, we examined NK cell activation influenced by a postbiotics mixture in response to gut microbiome modulation in stress-induced mice.					
35283424	0	2	theme	Microbiome	74:83	arg1	Regulation					85:94	Gut Microbiome Regulation	70:94	Gut Microbiome Regulation	70:94	Postbiotics Enhance NK Cell Activation in Stress-Induced Mice through Gut Microbiome Regulation.					
35283424	7	3	theme	cell	1035:1038	arg1	activation					1040:1049	the postbiotics mixture-induced NK cell activation	1000:1049	the postbiotics mixture-induced NK cell activation	1000:1049	Although the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice remains unclear, the postbiotics mixture-induced NK cell activation might have affected gut microbiome modulation.					
35283424	4	4	theme	cells	553:557	arg1	activation					536:545	In vivo activation	528:545	In vivo activation of NK cells	528:557	In vivo activation of NK cells increased in the postbiotics mixture treatment group in accordance with Th1/Th2 expression level.					
35283424	5	5	theme	reference	703:711	arg1	Meanwhile					657:665	Meanwhile	657:665	Meanwhile	657:665	Meanwhile, the Red Ginseng treatment group, a reference group, showed very little expression of NK cell activation.					
35283424	5	5	theme	reference	703:711	arg1	group					713:717	a reference group	701:717	a reference group	701:717	Meanwhile, the Red Ginseng treatment group, a reference group, showed very little expression of NK cell activation.					
35283424	6	6	theme	microbiome	853:862	arg1	composition					864:874	the gut microbiome composition	845:874	the gut microbiome composition	845:874	Moreover, the postbiotics mixture treatment group in particular changed the gut microbiome composition.					
35283424	7	7	theme	postbiotics	908:918	arg1	mixture					920:926	the postbiotics mixture	904:926	the postbiotics mixture	904:926	Although the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice remains unclear, the postbiotics mixture-induced NK cell activation might have affected gut microbiome modulation.					
35283424	3	8	theme	stress-induced	507:520	arg1	mice					522:525	stress-induced mice	507:525	stress-induced mice	507:525	In this study, we examined NK cell activation influenced by a postbiotics mixture in response to gut microbiome modulation in stress-induced mice.					
35283424	4	9	theme	NK	550:551	arg1	cells					553:557	NK cells	550:557	NK cells	550:557	In vivo activation of NK cells increased in the postbiotics mixture treatment group in accordance with Th1/Th2 expression level.					
35283424	4	10	dep	In	528:529	arg1	vivo					531:534	vivo	531:534	vivo	531:534	In vivo activation of NK cells increased in the postbiotics mixture treatment group in accordance with Th1/Th2 expression level.					
35283424	7	11	theme	gut	1071:1073	arg1	modulation					1086:1095	gut microbiome modulation	1071:1095	gut microbiome modulation	1071:1095	Although the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice remains unclear, the postbiotics mixture-induced NK cell activation might have affected gut microbiome modulation.					
35283424	7	12	theme	mixture	920:926	arg1	role					896:899	the exact role	886:899	the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice	886:981	Although the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice remains unclear, the postbiotics mixture-induced NK cell activation might have affected gut microbiome modulation.					
35283424	3	13	theme	microbiome	482:491	arg1	modulation					493:502	gut microbiome modulation	478:502	gut microbiome modulation in stress-induced mice	478:525	In this study, we examined NK cell activation influenced by a postbiotics mixture in response to gut microbiome modulation in stress-induced mice.					
35283424	4	14	theme	expression	639:648	arg1	level					650:654	Th1/Th2 expression level	631:654	Th1/Th2 expression level	631:654	In vivo activation of NK cells increased in the postbiotics mixture treatment group in accordance with Th1/Th2 expression level.					
35283424	2	15	theme	adaptive	362:369	arg1	immunity					371:378	innate and adaptive immunity	351:378	innate and adaptive immunity	351:378	Natural killer (NK) cells play a key role in innate and adaptive immunity.					
35283424	5	16	theme	NK	753:754	arg1	activation					761:770	NK cell activation	753:770	NK cell activation	753:770	Meanwhile, the Red Ginseng treatment group, a reference group, showed very little expression of NK cell activation.					
35283424	7	17	theme	microbiome	1075:1084	arg1	modulation					1086:1095	gut microbiome modulation	1071:1095	gut microbiome modulation	1071:1095	Although the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice remains unclear, the postbiotics mixture-induced NK cell activation might have affected gut microbiome modulation.					
35283424	1	18	theme	various	182:188	arg1	conditions					190:199	various conditions	182:199	various conditions	182:199	Recent studies have revealed that probiotics and their metabolites are present under various conditions; however, the role of probiotic metabolites (i.e., postbiotics in pathological states) is controversial.					
35283424	5	19	theme	cell	756:759	arg1	activation					761:770	NK cell activation	753:770	NK cell activation	753:770	Meanwhile, the Red Ginseng treatment group, a reference group, showed very little expression of NK cell activation.					
35283424	7	20	theme	NK	1032:1033	arg1	activation					1040:1049	the postbiotics mixture-induced NK cell activation	1000:1049	the postbiotics mixture-induced NK cell activation	1000:1049	Although the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice remains unclear, the postbiotics mixture-induced NK cell activation might have affected gut microbiome modulation.					
35283424	2	21	theme	killer	314:319	arg1	cells					326:330	Natural killer (NK) cells	306:330	Natural killer (NK) cells	306:330	Natural killer (NK) cells play a key role in innate and adaptive immunity.					
35283424	6	22	theme	gut	849:851	arg1	composition					864:874	the gut microbiome composition	845:874	the gut microbiome composition	845:874	Moreover, the postbiotics mixture treatment group in particular changed the gut microbiome composition.					
35283424	2	23	theme	innate	351:356	arg1	immunity					371:378	innate and adaptive immunity	351:378	innate and adaptive immunity	351:378	Natural killer (NK) cells play a key role in innate and adaptive immunity.					
35283424	5	24	theme	activation	761:770	arg1	expression					739:748	very little expression	727:748	very little expression of NK cell activation	727:770	Meanwhile, the Red Ginseng treatment group, a reference group, showed very little expression of NK cell activation.					
35283424	1	25	theme	pathological	267:278	arg1	states					280:285	pathological states	267:285	pathological states	267:285	Recent studies have revealed that probiotics and their metabolites are present under various conditions; however, the role of probiotic metabolites (i.e., postbiotics in pathological states) is controversial.					
35283424	7	26	theme	exact	890:894	arg1	role					896:899	the exact role	886:899	the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice	886:981	Although the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice remains unclear, the postbiotics mixture-induced NK cell activation might have affected gut microbiome modulation.					
35283424	2	27	theme	Natural	306:312	arg1	NK					322:323	NK	322:323	NK	322:323	Natural killer (NK) cells play a key role in innate and adaptive immunity.					
35283424	2	27	theme	Natural	306:312	arg1	killer					314:319	Natural killer	306:319	Natural killer (NK) cells	306:330	Natural killer (NK) cells play a key role in innate and adaptive immunity.					
35283424	4	28	theme	Th1/Th2	631:637	arg1	level					650:654	Th1/Th2 expression level	631:654	Th1/Th2 expression level	631:654	In vivo activation of NK cells increased in the postbiotics mixture treatment group in accordance with Th1/Th2 expression level.					
35283424	0	29	theme	Cell	23:26	arg1	Activation					28:37	NK Cell Activation	20:37	NK Cell Activation	20:37	Postbiotics Enhance NK Cell Activation in Stress-Induced Mice through Gut Microbiome Regulation.					
35283424	1	30	from	postbiotics	252:262	arg1	states					280:285	pathological states	267:285	pathological states	267:285	Recent studies have revealed that probiotics and their metabolites are present under various conditions; however, the role of probiotic metabolites (i.e., postbiotics in pathological states) is controversial.					
35283424	6	31	from	group	817:821	arg1	particular					826:835	particular	826:835	particular	826:835	Moreover, the postbiotics mixture treatment group in particular changed the gut microbiome composition.					
35283424	4	32	theme	treatment	596:604	arg1	group					606:610	the postbiotics mixture treatment group	572:610	the postbiotics mixture treatment group	572:610	In vivo activation of NK cells increased in the postbiotics mixture treatment group in accordance with Th1/Th2 expression level.					
35283424	1	33	theme	Recent	97:102	arg1	studies					104:110	Recent studies	97:110	Recent studies	97:110	Recent studies have revealed that probiotics and their metabolites are present under various conditions; however, the role of probiotic metabolites (i.e., postbiotics in pathological states) is controversial.					
35283424	6	34	theme	mixture	799:805	arg1	group					817:821	the postbiotics mixture treatment group	783:821	the postbiotics mixture treatment group in particular	783:835	Moreover, the postbiotics mixture treatment group in particular changed the gut microbiome composition.					
35283424	5	35	theme	Red	672:674	arg1	Meanwhile					657:665	Meanwhile	657:665	Meanwhile	657:665	Meanwhile, the Red Ginseng treatment group, a reference group, showed very little expression of NK cell activation.					
35283424	5	35	theme	Red	672:674	arg1	group					694:698	the Red Ginseng treatment group	668:698	the Red Ginseng treatment group	668:698	Meanwhile, the Red Ginseng treatment group, a reference group, showed very little expression of NK cell activation.					
35283424	1	36	dep	metabolites	233:243	arg1	postbiotics					252:262	postbiotics	252:262	postbiotics in pathological states	252:285	Recent studies have revealed that probiotics and their metabolites are present under various conditions; however, the role of probiotic metabolites (i.e., postbiotics in pathological states) is controversial.					
35283424	4	37	theme	mixture	588:594	arg1	group					606:610	the postbiotics mixture treatment group	572:610	the postbiotics mixture treatment group	572:610	In vivo activation of NK cells increased in the postbiotics mixture treatment group in accordance with Th1/Th2 expression level.					
35283424	5	38	theme	little	732:737	arg1	expression					739:748	very little expression	727:748	very little expression of NK cell activation	727:770	Meanwhile, the Red Ginseng treatment group, a reference group, showed very little expression of NK cell activation.					
35283424	4	39	theme	In	528:529	arg1	activation					536:545	In vivo activation	528:545	In vivo activation of NK cells	528:557	In vivo activation of NK cells increased in the postbiotics mixture treatment group in accordance with Th1/Th2 expression level.					
35283424	5	40	theme	Ginseng	676:682	arg1	Meanwhile					657:665	Meanwhile	657:665	Meanwhile	657:665	Meanwhile, the Red Ginseng treatment group, a reference group, showed very little expression of NK cell activation.					
35283424	5	40	theme	Ginseng	676:682	arg1	group					694:698	the Red Ginseng treatment group	668:698	the Red Ginseng treatment group	668:698	Meanwhile, the Red Ginseng treatment group, a reference group, showed very little expression of NK cell activation.					
35283424	3	41	theme	NK	408:409	arg1	activation					416:425	NK cell activation	408:425	NK cell activation	408:425	In this study, we examined NK cell activation influenced by a postbiotics mixture in response to gut microbiome modulation in stress-induced mice.					
35283424	5	42	theme	treatment	684:692	arg1	Meanwhile					657:665	Meanwhile	657:665	Meanwhile	657:665	Meanwhile, the Red Ginseng treatment group, a reference group, showed very little expression of NK cell activation.					
35283424	5	42	theme	treatment	684:692	arg1	group					694:698	the Red Ginseng treatment group	668:698	the Red Ginseng treatment group	668:698	Meanwhile, the Red Ginseng treatment group, a reference group, showed very little expression of NK cell activation.					
35283424	4	43	theme	postbiotics	576:586	arg1	group					606:610	the postbiotics mixture treatment group	572:610	the postbiotics mixture treatment group	572:610	In vivo activation of NK cells increased in the postbiotics mixture treatment group in accordance with Th1/Th2 expression level.					
35283424	3	44	theme	cell	411:414	arg1	activation					416:425	NK cell activation	408:425	NK cell activation	408:425	In this study, we examined NK cell activation influenced by a postbiotics mixture in response to gut microbiome modulation in stress-induced mice.					
35283424	7	45	theme	postbiotics	1004:1014	arg1	activation					1040:1049	the postbiotics mixture-induced NK cell activation	1000:1049	the postbiotics mixture-induced NK cell activation	1000:1049	Although the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice remains unclear, the postbiotics mixture-induced NK cell activation might have affected gut microbiome modulation.					
35283424	6	46	theme	treatment	807:815	arg1	group					817:821	the postbiotics mixture treatment group	783:821	the postbiotics mixture treatment group in particular	783:835	Moreover, the postbiotics mixture treatment group in particular changed the gut microbiome composition.					
35283424	3	47	theme	postbiotics	443:453	arg1	mixture					455:461	a postbiotics mixture	441:461	a postbiotics mixture	441:461	In this study, we examined NK cell activation influenced by a postbiotics mixture in response to gut microbiome modulation in stress-induced mice.					
35283424	0	48	theme	Stress-Induced	42:55	arg1	Mice					57:60	Stress-Induced Mice	42:60	Stress-Induced Mice	42:60	Postbiotics Enhance NK Cell Activation in Stress-Induced Mice through Gut Microbiome Regulation.					
35283424	7	49	theme	mixture-induced	1016:1030	arg1	activation					1040:1049	the postbiotics mixture-induced NK cell activation	1000:1049	the postbiotics mixture-induced NK cell activation	1000:1049	Although the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice remains unclear, the postbiotics mixture-induced NK cell activation might have affected gut microbiome modulation.					
35283424	1	50	dep	postbiotics	252:262	arg1	i.e.					246:249	i.e.	246:249	i.e.	246:249	Recent studies have revealed that probiotics and their metabolites are present under various conditions; however, the role of probiotic metabolites (i.e., postbiotics in pathological states) is controversial.					
35283424	3	51	dep	examined	399:406	arg1	influenced					427:436	influenced	427:436	examined NK cell activation influenced by a postbiotics mixture in response to gut microbiome modulation in stress-induced mice	399:525	In this study, we examined NK cell activation influenced by a postbiotics mixture in response to gut microbiome modulation in stress-induced mice.					
35283424	7	52	theme	stress-induced	963:976	arg1	mice					978:981	stress-induced mice	963:981	stress-induced mice	963:981	Although the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice remains unclear, the postbiotics mixture-induced NK cell activation might have affected gut microbiome modulation.					
35283424	3	53	dep	modulation	493:502	arg1	response					466:473	response	466:473	response	466:473	In this study, we examined NK cell activation influenced by a postbiotics mixture in response to gut microbiome modulation in stress-induced mice.					
35283424	6	54	theme	postbiotics	787:797	arg1	group					817:821	the postbiotics mixture treatment group	783:821	the postbiotics mixture treatment group in particular	783:835	Moreover, the postbiotics mixture treatment group in particular changed the gut microbiome composition.					
35283424	7	55	theme	mice	978:981	arg1	system					953:958	the immune system	942:958	the immune system of stress-induced mice	942:981	Although the exact role of the postbiotics mixture in regulating the immune system of stress-induced mice remains unclear, the postbiotics mixture-induced NK cell activation might have affected gut microbiome modulation.					
35283424	2	56	theme	key	339:341	arg1	role					343:346	a key role	337:346	a key role	337:346	Natural killer (NK) cells play a key role in innate and adaptive immunity.					
35283424	1	57	theme	probiotic	223:231	arg1	metabolites					233:243	probiotic metabolites	223:243	probiotic metabolites (i.e., postbiotics in pathological states)	223:286	Recent studies have revealed that probiotics and their metabolites are present under various conditions; however, the role of probiotic metabolites (i.e., postbiotics in pathological states) is controversial.					
35283424	0	58	theme	Gut	70:72	arg1	Regulation					85:94	Gut Microbiome Regulation	70:94	Gut Microbiome Regulation	70:94	Postbiotics Enhance NK Cell Activation in Stress-Induced Mice through Gut Microbiome Regulation.					
35283424	1	59	theme	metabolites	233:243	arg1	controversial					291:303	controversial	291:303	controversial	291:303	Recent studies have revealed that probiotics and their metabolites are present under various conditions; however, the role of probiotic metabolites (i.e., postbiotics in pathological states) is controversial.					
35283424	1	59	theme	metabolites	233:243	arg1	role					215:218	the role	211:218	the role of probiotic metabolites (i.e., postbiotics in pathological states)	211:286	Recent studies have revealed that probiotics and their metabolites are present under various conditions; however, the role of probiotic metabolites (i.e., postbiotics in pathological states) is controversial.					
35283424	0	60	theme	NK	20:21	arg1	Activation					28:37	NK Cell Activation	20:37	NK Cell Activation	20:37	Postbiotics Enhance NK Cell Activation in Stress-Induced Mice through Gut Microbiome Regulation.					
35283424	3	61	theme	gut	478:480	arg1	modulation					493:502	gut microbiome modulation	478:502	gut microbiome modulation in stress-induced mice	478:525	In this study, we examined NK cell activation influenced by a postbiotics mixture in response to gut microbiome modulation in stress-induced mice.					
35270006	0	0	theme	Exclusive	90:98	arg1	Breastfeeding					100:112	Exclusive Breastfeeding	90:112	Exclusive Breastfeeding	90:112	Human Milk Oligosaccharides and Bacterial Profile Modulate Infant Body Composition during Exclusive Breastfeeding.					
35270006	8	1	theme	maternal	1018:1025	arg1	status					1036:1041	maternal secretor status	1018:1041	maternal secretor status	1018:1041	Furthermore, when data were stratified based on maternal secretor status, some of these relationships differed significantly among infants born to secretor vs non-secretor mothers.					
35270006	1	2	theme	fundamental	162:172	arg1	ecosystem					152:160	a complex and variable ecosystem	129:160	a complex and variable ecosystem fundamental to the development of newborns	129:203	Human milk is a complex and variable ecosystem fundamental to the development of newborns.					
35270006	1	2	theme	fundamental	162:172	arg1	milk					121:124	Human milk	115:124	Human milk	115:124	Human milk is a complex and variable ecosystem fundamental to the development of newborns.					
35270006	9	3	theme	milk	1196:1199	arg1	profile					1211:1217	the human milk bacterial profile and HMO intakes	1186:1233	profile	1211:1217	In conclusion, in this pilot study the human milk bacterial profile and HMO intakes and concentrations were significantly associated with infant body composition, with associations modified by secretor status.					
35270006	10	4	theme	infant	1480:1485	arg1	composition					1492:1502	infant body composition	1480:1502	infant body composition	1480:1502	Future research designed to increase the understanding of the mechanisms by which HMO and human milk bacteria modulate infant body composition should include intakes in addition to concentrations.					
35270006	7	5	theme	fat-free	940:947	arg1	mass					949:952	fat-free mass	940:952	fat-free mass	940:952	Individual human milk bacteria and HMO intakes and concentrations were also significantly associated with infant anthropometry, fat-free mass, and adiposity.					
35270006	2	6	theme	human	298:302	arg1	profiles					319:326	human milk bacterial profiles	298:326	human milk bacterial profiles	298:326	This study aimed to investigate relationships between human milk oligosaccharides (HMO) and human milk bacterial profiles and infant body composition.					
35270006	10	7	dep	modulate	1471:1478	arg1	include					1511:1517	include	1511:1517	modulate infant body composition should include intakes in addition to concentrations	1471:1555	Future research designed to increase the understanding of the mechanisms by which HMO and human milk bacteria modulate infant body composition should include intakes in addition to concentrations.					
35270006	6	8	gly	sialylated	795:804	arg1	HMOs					806:809	several fucosylated and sialylated HMOs	771:809	several fucosylated and sialylated HMOs	771:809	Relative abundance of human milk bacterial taxa were significantly associated with concentrations of several fucosylated and sialylated HMOs.					
35270006	10	9	theme	human	1451:1455	arg1	milk					1457:1460	human milk	1451:1460	human milk	1451:1460	Future research designed to increase the understanding of the mechanisms by which HMO and human milk bacteria modulate infant body composition should include intakes in addition to concentrations.					
35270006	9	10	theme	human	1190:1194	arg1	profile					1211:1217	the human milk bacterial profile and HMO intakes	1186:1233	profile	1211:1217	In conclusion, in this pilot study the human milk bacterial profile and HMO intakes and concentrations were significantly associated with infant body composition, with associations modified by secretor status.					
35270006	9	11	theme	pilot	1174:1178	arg1	study					1180:1184	this pilot study	1169:1184	this pilot study	1169:1184	In conclusion, in this pilot study the human milk bacterial profile and HMO intakes and concentrations were significantly associated with infant body composition, with associations modified by secretor status.					
35270006	10	12	dep	HMO	1443:1445	arg1	bacteria					1462:1469	bacteria	1462:1469	bacteria	1462:1469	Future research designed to increase the understanding of the mechanisms by which HMO and human milk bacteria modulate infant body composition should include intakes in addition to concentrations.					
35270006	7	13	theme	infant	918:923	arg1	anthropometry					925:937	infant anthropometry	918:937	infant anthropometry	918:937	Individual human milk bacteria and HMO intakes and concentrations were also significantly associated with infant anthropometry, fat-free mass, and adiposity.					
35270006	3	14	theme	Human	357:361	arg1	n					377:377	n = 60	377:382	n = 60	377:382	Human milk samples (n = 60) were collected at two months postpartum.					
35270006	3	14	theme	Human	357:361	arg1	samples					368:374	Human milk samples	357:374	Human milk samples (n = 60)	357:383	Human milk samples (n = 60) were collected at two months postpartum.					
35270006	5	15	theme	high-performance	630:645	arg1	chromatography					654:667	high-performance liquid chromatography	630:667	high-performance liquid chromatography	630:667	Human milk bacterial profiles were assessed using full-length 16S rRNA gene sequencing and 19 HMOs were quantitated using high-performance liquid chromatography.					
35270006	9	16	theme	bacterial	1201:1209	arg1	profile					1211:1217	the human milk bacterial profile and HMO intakes	1186:1233	profile	1211:1217	In conclusion, in this pilot study the human milk bacterial profile and HMO intakes and concentrations were significantly associated with infant body composition, with associations modified by secretor status.					
35270006	3	17	theme	milk	363:366	arg1	n					377:377	n = 60	377:382	n = 60	377:382	Human milk samples (n = 60) were collected at two months postpartum.					
35270006	3	17	theme	milk	363:366	arg1	samples					368:374	Human milk samples	357:374	Human milk samples (n = 60)	357:383	Human milk samples (n = 60) were collected at two months postpartum.					
35270006	4	18	theme	bioimpedance	481:492	arg1	spectroscopy					494:505	bioimpedance spectroscopy	481:505	bioimpedance spectroscopy	481:505	Infant and maternal body composition was measured with bioimpedance spectroscopy.					
35270006	5	19	theme	liquid	647:652	arg1	chromatography					654:667	high-performance liquid chromatography	630:667	high-performance liquid chromatography	630:667	Human milk bacterial profiles were assessed using full-length 16S rRNA gene sequencing and 19 HMOs were quantitated using high-performance liquid chromatography.					
35270006	5	20	theme	gene	579:582	arg1	sequencing					584:593	full-length 16S rRNA gene sequencing	558:593	full-length 16S rRNA gene sequencing	558:593	Human milk bacterial profiles were assessed using full-length 16S rRNA gene sequencing and 19 HMOs were quantitated using high-performance liquid chromatography.					
35270006	7	21	theme	Individual	812:821	arg1	bacteria					834:841	Individual human milk bacteria	812:841	Individual human milk bacteria	812:841	Individual human milk bacteria and HMO intakes and concentrations were also significantly associated with infant anthropometry, fat-free mass, and adiposity.					
35270006	5	22	theme	Human	508:512	arg1	profiles					529:536	Human milk bacterial profiles	508:536	Human milk bacterial profiles	508:536	Human milk bacterial profiles were assessed using full-length 16S rRNA gene sequencing and 19 HMOs were quantitated using high-performance liquid chromatography.					
35270006	0	23	theme	Milk	6:9	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides and Bacterial Profile Modulate Infant Body Composition during Exclusive Breastfeeding.					
35270006	5	24	theme	milk	514:517	arg1	profiles					529:536	Human milk bacterial profiles	508:536	Human milk bacterial profiles	508:536	Human milk bacterial profiles were assessed using full-length 16S rRNA gene sequencing and 19 HMOs were quantitated using high-performance liquid chromatography.					
35270006	3	25	theme	months	407:412	arg1	postpartum					414:423	two months postpartum	403:423	two months postpartum	403:423	Human milk samples (n = 60) were collected at two months postpartum.					
35270006	0	26	theme	Human	0:4	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides and Bacterial Profile Modulate Infant Body Composition during Exclusive Breastfeeding.					
35270006	8	27	theme	non-secretor	1129:1140	arg1	mothers					1142:1148	secretor vs non-secretor mothers	1117:1148	secretor vs non-secretor mothers	1117:1148	Furthermore, when data were stratified based on maternal secretor status, some of these relationships differed significantly among infants born to secretor vs non-secretor mothers.					
35270006	9	28	theme	infant	1289:1294	arg1	composition					1301:1311	infant body composition	1289:1311	infant body composition	1289:1311	In conclusion, in this pilot study the human milk bacterial profile and HMO intakes and concentrations were significantly associated with infant body composition, with associations modified by secretor status.					
35270006	1	29	theme	newborns	196:203	arg1	development					181:191	the development	177:191	the development of newborns	177:203	Human milk is a complex and variable ecosystem fundamental to the development of newborns.					
35270006	9	30	theme	body	1296:1299	arg1	composition					1301:1311	infant body composition	1289:1311	infant body composition	1289:1311	In conclusion, in this pilot study the human milk bacterial profile and HMO intakes and concentrations were significantly associated with infant body composition, with associations modified by secretor status.					
35270006	2	31	theme	bacterial	309:317	arg1	profiles					319:326	human milk bacterial profiles	298:326	human milk bacterial profiles	298:326	This study aimed to investigate relationships between human milk oligosaccharides (HMO) and human milk bacterial profiles and infant body composition.					
35270006	6	32	theme	taxa	713:716	arg1	abundance					679:687	Relative abundance	670:687	Relative abundance of human milk bacterial taxa	670:716	Relative abundance of human milk bacterial taxa were significantly associated with concentrations of several fucosylated and sialylated HMOs.					
35270006	10	33	theme	body	1487:1490	arg1	composition					1492:1502	infant body composition	1480:1502	infant body composition	1480:1502	Future research designed to increase the understanding of the mechanisms by which HMO and human milk bacteria modulate infant body composition should include intakes in addition to concentrations.					
35270006	3	34	theme	=	379:379	arg1	n					377:377	n = 60	377:382	n = 60	377:382	Human milk samples (n = 60) were collected at two months postpartum.					
35270006	3	34	theme	=	379:379	arg1	samples					368:374	Human milk samples	357:374	Human milk samples (n = 60)	357:383	Human milk samples (n = 60) were collected at two months postpartum.					
35270006	7	35	theme	HMO	847:849	arg1	intakes					851:857	HMO intakes	847:857	HMO intakes	847:857	Individual human milk bacteria and HMO intakes and concentrations were also significantly associated with infant anthropometry, fat-free mass, and adiposity.					
35270006	6	36	theme	HMOs	806:809	arg1	concentrations					753:766	concentrations	753:766	concentrations of several fucosylated and sialylated HMOs	753:809	Relative abundance of human milk bacterial taxa were significantly associated with concentrations of several fucosylated and sialylated HMOs.					
35270006	4	37	theme	maternal	437:444	arg1	composition					451:461	maternal body composition	437:461	maternal body composition	437:461	Infant and maternal body composition was measured with bioimpedance spectroscopy.					
35270006	2	38	theme	milk	304:307	arg1	profiles					319:326	human milk bacterial profiles	298:326	human milk bacterial profiles	298:326	This study aimed to investigate relationships between human milk oligosaccharides (HMO) and human milk bacterial profiles and infant body composition.					
35270006	6	39	theme	bacterial	703:711	arg1	taxa					713:716	human milk bacterial taxa	692:716	human milk bacterial taxa	692:716	Relative abundance of human milk bacterial taxa were significantly associated with concentrations of several fucosylated and sialylated HMOs.					
35270006	6	40	theme	milk	698:701	arg1	taxa					713:716	human milk bacterial taxa	692:716	human milk bacterial taxa	692:716	Relative abundance of human milk bacterial taxa were significantly associated with concentrations of several fucosylated and sialylated HMOs.					
35270006	0	41	theme	Bacterial	32:40	arg1	Profile					42:48	Bacterial Profile	32:48	Bacterial Profile	32:48	Human Milk Oligosaccharides and Bacterial Profile Modulate Infant Body Composition during Exclusive Breastfeeding.					
35270006	10	42	theme	Future	1361:1366	arg1	research					1368:1375	Future research	1361:1375	Future research	1361:1375	Future research designed to increase the understanding of the mechanisms by which HMO and human milk bacteria modulate infant body composition should include intakes in addition to concentrations.					
35270006	0	43	theme	Infant	59:64	arg1	Composition					71:81	Infant Body Composition	59:81	Infant Body Composition	59:81	Human Milk Oligosaccharides and Bacterial Profile Modulate Infant Body Composition during Exclusive Breastfeeding.					
35270006	4	44	theme	body	446:449	arg1	composition					451:461	maternal body composition	437:461	maternal body composition	437:461	Infant and maternal body composition was measured with bioimpedance spectroscopy.					
35270006	5	45	theme	full-length	558:568	arg1	sequencing					584:593	full-length 16S rRNA gene sequencing	558:593	full-length 16S rRNA gene sequencing	558:593	Human milk bacterial profiles were assessed using full-length 16S rRNA gene sequencing and 19 HMOs were quantitated using high-performance liquid chromatography.					
35270006	7	46	theme	human	823:827	arg1	bacteria					834:841	Individual human milk bacteria	812:841	Individual human milk bacteria	812:841	Individual human milk bacteria and HMO intakes and concentrations were also significantly associated with infant anthropometry, fat-free mass, and adiposity.					
35270006	8	47	theme	secretor	1117:1124	arg1	mothers					1142:1148	secretor vs non-secretor mothers	1117:1148	secretor vs non-secretor mothers	1117:1148	Furthermore, when data were stratified based on maternal secretor status, some of these relationships differed significantly among infants born to secretor vs non-secretor mothers.					
35270006	5	48	theme	16S	570:572	arg1	sequencing					584:593	full-length 16S rRNA gene sequencing	558:593	full-length 16S rRNA gene sequencing	558:593	Human milk bacterial profiles were assessed using full-length 16S rRNA gene sequencing and 19 HMOs were quantitated using high-performance liquid chromatography.					
35270006	6	49	theme	human	692:696	arg1	taxa					713:716	human milk bacterial taxa	692:716	human milk bacterial taxa	692:716	Relative abundance of human milk bacterial taxa were significantly associated with concentrations of several fucosylated and sialylated HMOs.					
35270006	7	50	theme	milk	829:832	arg1	bacteria					834:841	Individual human milk bacteria	812:841	Individual human milk bacteria	812:841	Individual human milk bacteria and HMO intakes and concentrations were also significantly associated with infant anthropometry, fat-free mass, and adiposity.					
35270006	10	51	theme	mechanisms	1423:1432	arg1	understanding					1402:1414	the understanding	1398:1414	the understanding of the mechanisms by which HMO and human milk bacteria modulate infant body composition should include intakes in addition to concentrations	1398:1555	Future research designed to increase the understanding of the mechanisms by which HMO and human milk bacteria modulate infant body composition should include intakes in addition to concentrations.					
35270006	8	52	theme	secretor	1027:1034	arg1	status					1036:1041	maternal secretor status	1018:1041	maternal secretor status	1018:1041	Furthermore, when data were stratified based on maternal secretor status, some of these relationships differed significantly among infants born to secretor vs non-secretor mothers.					
35270006	5	53	theme	rRNA	574:577	arg1	sequencing					584:593	full-length 16S rRNA gene sequencing	558:593	full-length 16S rRNA gene sequencing	558:593	Human milk bacterial profiles were assessed using full-length 16S rRNA gene sequencing and 19 HMOs were quantitated using high-performance liquid chromatography.					
35270006	1	54	theme	complex	131:137	arg1	ecosystem					152:160	a complex and variable ecosystem	129:160	a complex and variable ecosystem fundamental to the development of newborns	129:203	Human milk is a complex and variable ecosystem fundamental to the development of newborns.					
35270006	1	54	theme	complex	131:137	arg1	milk					121:124	Human milk	115:124	Human milk	115:124	Human milk is a complex and variable ecosystem fundamental to the development of newborns.					
35270006	1	55	theme	Human	115:119	arg1	ecosystem					152:160	a complex and variable ecosystem	129:160	a complex and variable ecosystem fundamental to the development of newborns	129:203	Human milk is a complex and variable ecosystem fundamental to the development of newborns.					
35270006	1	55	theme	Human	115:119	arg1	milk					121:124	Human milk	115:124	Human milk	115:124	Human milk is a complex and variable ecosystem fundamental to the development of newborns.					
35270006	6	56	gly	fucosylated	779:789	arg1	HMOs					806:809	several fucosylated and sialylated HMOs	771:809	several fucosylated and sialylated HMOs	771:809	Relative abundance of human milk bacterial taxa were significantly associated with concentrations of several fucosylated and sialylated HMOs.					
35270006	6	57	theme	sialylated	795:804	arg1	HMOs					806:809	several fucosylated and sialylated HMOs	771:809	several fucosylated and sialylated HMOs	771:809	Relative abundance of human milk bacterial taxa were significantly associated with concentrations of several fucosylated and sialylated HMOs.					
35270006	9	58	theme	secretor	1344:1351	arg1	status					1353:1358	secretor status	1344:1358	secretor status	1344:1358	In conclusion, in this pilot study the human milk bacterial profile and HMO intakes and concentrations were significantly associated with infant body composition, with associations modified by secretor status.					
35270006	9	59	theme	HMO	1223:1225	arg1	intakes					1227:1233	the human milk bacterial profile and HMO intakes	1186:1233	intakes	1227:1233	In conclusion, in this pilot study the human milk bacterial profile and HMO intakes and concentrations were significantly associated with infant body composition, with associations modified by secretor status.					
35270006	5	60	theme	bacterial	519:527	arg1	profiles					529:536	Human milk bacterial profiles	508:536	Human milk bacterial profiles	508:536	Human milk bacterial profiles were assessed using full-length 16S rRNA gene sequencing and 19 HMOs were quantitated using high-performance liquid chromatography.					
35270006	2	61	theme	infant	332:337	arg1	composition					344:354	infant body composition	332:354	infant body composition	332:354	This study aimed to investigate relationships between human milk oligosaccharides (HMO) and human milk bacterial profiles and infant body composition.					
35270006	2	62	theme	body	339:342	arg1	composition					344:354	infant body composition	332:354	infant body composition	332:354	This study aimed to investigate relationships between human milk oligosaccharides (HMO) and human milk bacterial profiles and infant body composition.					
35270006	2	63	theme	milk	266:269	arg1	HMO					289:291	HMO	289:291	HMO	289:291	This study aimed to investigate relationships between human milk oligosaccharides (HMO) and human milk bacterial profiles and infant body composition.					
35270006	2	63	theme	milk	266:269	arg1	oligosaccharides					271:286	human milk oligosaccharides	260:286	human milk oligosaccharides (HMO)	260:292	This study aimed to investigate relationships between human milk oligosaccharides (HMO) and human milk bacterial profiles and infant body composition.					
35270006	6	64	theme	fucosylated	779:789	arg1	HMOs					806:809	several fucosylated and sialylated HMOs	771:809	several fucosylated and sialylated HMOs	771:809	Relative abundance of human milk bacterial taxa were significantly associated with concentrations of several fucosylated and sialylated HMOs.					
35270006	1	65	theme	variable	143:150	arg1	ecosystem					152:160	a complex and variable ecosystem	129:160	a complex and variable ecosystem fundamental to the development of newborns	129:203	Human milk is a complex and variable ecosystem fundamental to the development of newborns.					
35270006	1	65	theme	variable	143:150	arg1	milk					121:124	Human milk	115:124	Human milk	115:124	Human milk is a complex and variable ecosystem fundamental to the development of newborns.					
35270006	6	66	theme	Relative	670:677	arg1	abundance					679:687	Relative abundance	670:687	Relative abundance of human milk bacterial taxa	670:716	Relative abundance of human milk bacterial taxa were significantly associated with concentrations of several fucosylated and sialylated HMOs.					
35270006	0	67	theme	Body	66:69	arg1	Composition					71:81	Infant Body Composition	59:81	Infant Body Composition	59:81	Human Milk Oligosaccharides and Bacterial Profile Modulate Infant Body Composition during Exclusive Breastfeeding.					
35270006	2	68	theme	human	260:264	arg1	HMO					289:291	HMO	289:291	HMO	289:291	This study aimed to investigate relationships between human milk oligosaccharides (HMO) and human milk bacterial profiles and infant body composition.					
35270006	2	68	theme	human	260:264	arg1	oligosaccharides					271:286	human milk oligosaccharides	260:286	human milk oligosaccharides (HMO)	260:292	This study aimed to investigate relationships between human milk oligosaccharides (HMO) and human milk bacterial profiles and infant body composition.					
35270006	6	69	theme	several	771:777	arg1	HMOs					806:809	several fucosylated and sialylated HMOs	771:809	several fucosylated and sialylated HMOs	771:809	Relative abundance of human milk bacterial taxa were significantly associated with concentrations of several fucosylated and sialylated HMOs.					
36283436	2	0	theme	energy	563:568	arg1	spectroscopy					581:592	energy dispersive spectroscopy	563:592	energy dispersive spectroscopy (EDS)	563:598	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	0	theme	energy	563:568	arg1	EDS					595:597	EDS	595:597	EDS	595:597	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	1	1	theme	Ce-Cu	248:252	arg1	copper					227:232	copper	227:232	copper	227:232	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	1	1	theme	Ce-Cu	248:252	arg1	Alg					254:256	Ce-Cu@Alg	248:256	Ce-Cu@Alg	248:256	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	8	2	theme	large	1481:1485	arg1	volumes					1496:1502	large effluent volumes	1481:1502	large effluent volumes	1481:1502	This study shows the possibility of using this new hybrid biocomposite in the industrial sector to treat large effluent volumes.					
36283436	3	3	theme	TBZ	675:677	arg1	biosorption					685:695	the TBZ batch biosorption	671:695	the TBZ batch biosorption by Ce-Cu@Alg composite	671:718	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	5	4	theme	180 min	1126:1132	arg1	100 ppm					1034:1040	100 ppm	1034:1040	100 ppm of initial TBZ concentration	1034:1069	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	5	4	theme	180 min	1126:1132	arg1	dose					1090:1093	a Ce-Cu@Alg beads dose	1072:1093	a Ce-Cu@Alg beads dose of 6 g/L	1072:1102	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	5	4	theme	180 min	1126:1132	arg1	time					1118:1121	a contact time	1108:1121	a contact time of 180 min for maximum removal of 83.42%	1108:1162	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	1	5	theme	biocomposite	168:179	arg1	engineering					145:155	The engineering	141:155	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads	141:280	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	7	6	theme	Thomas	1326:1331	arg1	model					1333:1337	The Thomas model	1322:1337	The Thomas model	1322:1337	The Thomas model was best fitted to the kinetic data.					
36283436	3	7	theme	Ce-Cu	700:704	arg1	composite					710:718	Ce-Cu@Alg composite	700:718	Ce-Cu@Alg composite	700:718	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	5	8	theme	RSM	988:990	arg1	approach					992:999	the RSM approach	984:999	the RSM approach coupled with the CCD design	984:1027	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	3	9	theme	biosorption	685:695	arg1	results					660:666	The results	656:666	The results of the TBZ batch biosorption by Ce-Cu@Alg composite	656:718	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	6	10	from	flow	1285:1288	arg1	sorption					1192:1199	the TBZ sorption	1184:1199	the TBZ sorption on a fixed bed of Ce-Cu@Alg beads	1184:1233	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	6	10	from	flow	1285:1288	arg1	effective					1239:1247	effective	1239:1247	effective	1239:1247	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	1	11	theme	edule	203:207	arg1	shells					209:214	Cerastoderma edule shells	190:214	Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads	190:280	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	8	12	from	biocomposite	1434:1445	arg1	sector					1465:1470	the industrial sector	1450:1470	the industrial sector	1450:1470	This study shows the possibility of using this new hybrid biocomposite in the industrial sector to treat large effluent volumes.					
36283436	3	13	theme	Alg	706:708	arg1	composite					710:718	Ce-Cu@Alg composite	700:718	Ce-Cu@Alg composite	700:718	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	6	14	from	height	1264:1269	arg1	sorption					1192:1199	the TBZ sorption	1184:1199	the TBZ sorption on a fixed bed of Ce-Cu@Alg beads	1184:1233	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	6	14	from	height	1264:1269	arg1	effective					1239:1247	effective	1239:1247	effective	1239:1247	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	3	15	with	adequate	764:771	arg1	value					844:848	a maximum adsorption capacity value	814:848	a maximum adsorption capacity value of 21.98 mg/g	814:862	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	3	16	theme	adsorption	789:798	arg1	process					800:806	the adsorption process	785:806	the adsorption process	785:806	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	1	17	from	water	359:363	arg1	pesticide					344:352	dynamic adsorption thiabendazole (TBZ) pesticide	305:352	dynamic adsorption thiabendazole (TBZ) pesticide	305:352	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	1	17	from	water	359:363	arg1	batch					295:299	batch	295:299	batch	295:299	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	0	18	theme	shells	61:66	arg1	Alginate					68:75	copper-doped Cerastoderma edule shells@Alginate	29:75	copper-doped Cerastoderma edule shells@Alginate	29:75	A novel hydrogel beads based copper-doped Cerastoderma edule shells@Alginate biocomposite for highly fungicide sorption from aqueous medium.					
36283436	5	19	theme	concentration	1057:1069	arg1	100 ppm					1034:1040	100 ppm	1034:1040	100 ppm of initial TBZ concentration	1034:1069	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	5	19	theme	concentration	1057:1069	arg1	dose					1090:1093	a Ce-Cu@Alg beads dose	1072:1093	a Ce-Cu@Alg beads dose of 6 g/L	1072:1102	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	5	19	theme	concentration	1057:1069	arg1	time					1118:1121	a contact time	1108:1121	a contact time of 180 min for maximum removal of 83.42%	1108:1162	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	5	19	theme	concentration	1057:1069	arg1	conditions					959:968	The optimal conditions	947:968	The optimal conditions determined by the RSM approach coupled with the CCD design	947:1027	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	0	20	theme	hydrogel	8:15	arg1	beads					17:21	A novel hydrogel beads	0:21	A novel hydrogel beads based copper-doped Cerastoderma edule shells@Alginate	0:75	A novel hydrogel beads based copper-doped Cerastoderma edule shells@Alginate biocomposite for highly fungicide sorption from aqueous medium.					
36283436	4	21	theme	pseudo-second-order	920:938	arg1	model					940:944	the pseudo-second-order model	916:944	the pseudo-second-order model	916:944	Moreover, the adsorption kinetics were adjusted by the pseudo-second-order model.					
36283436	5	22	theme	%	1162:1162	arg1	removal					1146:1152	maximum removal	1138:1152	maximum removal of 83.42%	1138:1162	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	1	23	theme	adsorption	313:322	arg1	pesticide					344:352	dynamic adsorption thiabendazole (TBZ) pesticide	305:352	dynamic adsorption thiabendazole (TBZ) pesticide	305:352	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	4	24	theme	adsorption	879:888	arg1	kinetics					890:897	the adsorption kinetics	875:897	the adsorption kinetics	875:897	Moreover, the adsorption kinetics were adjusted by the pseudo-second-order model.					
36283436	6	25	theme	Alg	1225:1227	arg1	beads					1229:1233	Ce-Cu@Alg beads	1219:1233	Ce-Cu@Alg beads	1219:1233	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	2	26	theme	X-ray	486:490	arg1	XRD					514:516	XRD	514:516	XRD	514:516	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	26	theme	X-ray	486:490	arg1	analysis					504:511	X-ray diffraction analysis	486:511	X-ray diffraction analysis (XRD)	486:517	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	0	27	theme	fungicide	101:109	arg1	sorption					111:118	highly fungicide sorption	94:118	highly fungicide sorption from aqueous medium	94:138	A novel hydrogel beads based copper-doped Cerastoderma edule shells@Alginate biocomposite for highly fungicide sorption from aqueous medium.					
36283436	5	28	theme	initial	1045:1051	arg1	concentration					1057:1069	initial TBZ concentration	1045:1069	initial TBZ concentration	1045:1069	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	0	29	theme	copper-doped	29:40	arg1	Alginate					68:75	copper-doped Cerastoderma edule shells@Alginate	29:75	copper-doped Cerastoderma edule shells@Alginate	29:75	A novel hydrogel beads based copper-doped Cerastoderma edule shells@Alginate biocomposite for highly fungicide sorption from aqueous medium.					
36283436	3	30	theme	adsorption	824:833	arg1	value					844:848	a maximum adsorption capacity value	814:848	a maximum adsorption capacity value of 21.98 mg/g	814:862	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	0	31	theme	@	67:67	arg1	Alginate					68:75	copper-doped Cerastoderma edule shells@Alginate	29:75	copper-doped Cerastoderma edule shells@Alginate	29:75	A novel hydrogel beads based copper-doped Cerastoderma edule shells@Alginate biocomposite for highly fungicide sorption from aqueous medium.					
36283436	2	32	theme	various	406:412	arg1	analysis					504:511	X-ray diffraction analysis	486:511	X-ray diffraction analysis (XRD)	486:517	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	32	theme	various	406:412	arg1	thermogravimetric					601:617	thermogravimetric	601:617	thermogravimetric	601:617	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	32	theme	various	406:412	arg1	analysis					543:550	Brunauer-Emmett-Teller analysis	520:550	Brunauer-Emmett-Teller analysis (BET)	520:556	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	32	theme	various	406:412	arg1	microscopy					468:477	scanning electron microscopy	450:477	scanning electron microscopy (SEM)	450:483	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	32	theme	various	406:412	arg1	techniques					431:440	various characterization techniques	406:440	various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA)	406:653	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	32	theme	various	406:412	arg1	spectroscopy					581:592	energy dispersive spectroscopy	563:592	energy dispersive spectroscopy (EDS)	563:598	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	32	theme	various	406:412	arg1	analysis					636:643	differential analysis	623:643	differential analysis (TGA-DTA)	623:653	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	33	theme	differential	623:634	arg1	analysis					636:643	differential analysis	623:643	differential analysis (TGA-DTA)	623:653	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	33	theme	differential	623:634	arg1	TGA-DTA					646:652	TGA-DTA	646:652	TGA-DTA	646:652	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	6	34	theme	effluent	1276:1283	arg1	flow					1285:1288	low effluent flow	1272:1288	low effluent flow	1272:1288	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	1	35	theme	hydrogel	267:274	arg1	beads					276:280	hydrogel beads	267:280	hydrogel beads	267:280	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	3	36	theme	21.98 mg/g	853:862	arg1	value					844:848	a maximum adsorption capacity value	814:848	a maximum adsorption capacity value of 21.98 mg/g	814:862	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	2	37	theme	electron	459:466	arg1	SEM					480:482	SEM	480:482	SEM	480:482	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	37	theme	electron	459:466	arg1	microscopy					468:477	scanning electron microscopy	450:477	scanning electron microscopy (SEM)	450:483	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	5	38	theme	@	1079:1079	arg1	beads					1084:1088	Ce-Cu@Alg beads	1074:1088	a Ce-Cu@Alg beads dose of 6 g/L	1072:1102	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	0	39	theme	Cerastoderma	42:53	arg1	Alginate					68:75	copper-doped Cerastoderma edule shells@Alginate	29:75	copper-doped Cerastoderma edule shells@Alginate	29:75	A novel hydrogel beads based copper-doped Cerastoderma edule shells@Alginate biocomposite for highly fungicide sorption from aqueous medium.					
36283436	5	40	theme	beads	1084:1088	arg1	100 ppm					1034:1040	100 ppm	1034:1040	100 ppm of initial TBZ concentration	1034:1069	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	5	40	theme	beads	1084:1088	arg1	dose					1090:1093	a Ce-Cu@Alg beads dose	1072:1093	a Ce-Cu@Alg beads dose of 6 g/L	1072:1102	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	8	41	theme	new	1423:1425	arg1	biocomposite					1434:1445	this new hybrid biocomposite	1418:1445	this new hybrid biocomposite in the industrial sector	1418:1470	This study shows the possibility of using this new hybrid biocomposite in the industrial sector to treat large effluent volumes.					
36283436	8	42	theme	effluent	1487:1494	arg1	volumes					1496:1502	large effluent volumes	1481:1502	large effluent volumes	1481:1502	This study shows the possibility of using this new hybrid biocomposite in the industrial sector to treat large effluent volumes.					
36283436	0	43	from	medium	133:138	arg1	sorption					111:118	highly fungicide sorption	94:118	highly fungicide sorption from aqueous medium	94:138	A novel hydrogel beads based copper-doped Cerastoderma edule shells@Alginate biocomposite for highly fungicide sorption from aqueous medium.					
36283436	2	44	theme	prepared	370:377	arg1	biosorbent					379:388	The prepared biosorbent	366:388	The prepared biosorbent	366:388	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	5	45	theme	optimal	951:957	arg1	100 ppm					1034:1040	100 ppm	1034:1040	100 ppm of initial TBZ concentration	1034:1069	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	5	45	theme	optimal	951:957	arg1	conditions					959:968	The optimal conditions	947:968	The optimal conditions determined by the RSM approach coupled with the CCD design	947:1027	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	5	46	theme	contact	1110:1116	arg1	100 ppm					1034:1040	100 ppm	1034:1040	100 ppm of initial TBZ concentration	1034:1069	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	5	46	theme	contact	1110:1116	arg1	time					1118:1121	a contact time	1108:1121	a contact time of 180 min for maximum removal of 83.42%	1108:1162	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	1	47	used	used	286:289	arg2	engineering					145:155	The engineering	141:155	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads	141:280	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	6	48	theme	other	1172:1176	arg1	hand					1178:1181	the other hand	1168:1181	the other hand	1168:1181	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	6	49	theme	solution	1298:1305	arg1	concentration					1307:1319	low solution concentration	1294:1319	low solution concentration	1294:1319	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	1	50	theme	@	253:253	arg1	copper					227:232	copper	227:232	copper	227:232	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	1	50	theme	@	253:253	arg1	Alg					254:256	Ce-Cu@Alg	248:256	Ce-Cu@Alg	248:256	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	3	51	theme	batch	679:683	arg1	biosorption					685:695	the TBZ batch biosorption	671:695	the TBZ batch biosorption by Ce-Cu@Alg composite	671:718	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	1	52	theme	novel	162:166	arg1	biocomposite					168:179	a novel biocomposite	160:179	a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads	160:280	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	6	53	theme	column	1257:1262	arg1	height					1264:1269	high column height	1252:1269	high column height	1252:1269	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	2	54	theme	dispersive	570:579	arg1	spectroscopy					581:592	energy dispersive spectroscopy	563:592	energy dispersive spectroscopy (EDS)	563:598	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	54	theme	dispersive	570:579	arg1	EDS					595:597	EDS	595:597	EDS	595:597	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	3	55	theme	@	705:705	arg1	composite					710:718	Ce-Cu@Alg composite	700:718	Ce-Cu@Alg composite	700:718	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	0	56	theme	aqueous	125:131	arg1	medium					133:138	aqueous medium	125:138	aqueous medium	125:138	A novel hydrogel beads based copper-doped Cerastoderma edule shells@Alginate biocomposite for highly fungicide sorption from aqueous medium.					
36283436	1	57	theme	Cerastoderma	190:201	arg1	shells					209:214	Cerastoderma edule shells	190:214	Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads	190:280	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	6	58	theme	fixed	1206:1210	arg1	bed					1212:1214	a fixed bed	1204:1214	a fixed bed of Ce-Cu@Alg beads	1204:1233	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	5	59	theme	CCD	1018:1020	arg1	design					1022:1027	the CCD design	1014:1027	the CCD design	1014:1027	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	5	60	theme	Ce-Cu	1074:1078	arg1	beads					1084:1088	Ce-Cu@Alg beads	1074:1088	a Ce-Cu@Alg beads dose of 6 g/L	1072:1102	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	6	61	theme	TBZ	1188:1190	arg1	sorption					1192:1199	the TBZ sorption	1184:1199	the TBZ sorption on a fixed bed of Ce-Cu@Alg beads	1184:1233	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	6	61	theme	TBZ	1188:1190	arg1	effective					1239:1247	effective	1239:1247	effective	1239:1247	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	2	62	theme	Brunauer-Emmett-Teller	520:541	arg1	analysis					543:550	Brunauer-Emmett-Teller analysis	520:550	Brunauer-Emmett-Teller analysis (BET)	520:556	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	62	theme	Brunauer-Emmett-Teller	520:541	arg1	BET					553:555	BET	553:555	BET	553:555	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	6	63	theme	high	1252:1255	arg1	height					1264:1269	high column height	1252:1269	high column height	1252:1269	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	3	64	theme	Langmuir	736:743	arg1	model					745:749	the Langmuir model	732:749	the Langmuir model	732:749	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	3	64	theme	Langmuir	736:743	arg1	adequate					764:771	adequate	764:771	adequate	764:771	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	1	65	theme	dynamic	305:311	arg1	pesticide					344:352	dynamic adsorption thiabendazole (TBZ) pesticide	305:352	dynamic adsorption thiabendazole (TBZ) pesticide	305:352	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	3	66	theme	maximum	816:822	arg1	value					844:848	a maximum adsorption capacity value	814:848	a maximum adsorption capacity value of 21.98 mg/g	814:862	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	6	67	theme	beads	1229:1233	arg1	bed					1212:1214	a fixed bed	1204:1214	a fixed bed of Ce-Cu@Alg beads	1204:1233	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	1	68	theme	thiabendazole	324:336	arg1	pesticide					344:352	dynamic adsorption thiabendazole (TBZ) pesticide	305:352	dynamic adsorption thiabendazole (TBZ) pesticide	305:352	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	2	69	theme	diffraction	492:502	arg1	XRD					514:516	XRD	514:516	XRD	514:516	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	69	theme	diffraction	492:502	arg1	analysis					504:511	X-ray diffraction analysis	486:511	X-ray diffraction analysis (XRD)	486:517	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	6	70	theme	@	1224:1224	arg1	beads					1229:1233	Ce-Cu@Alg beads	1219:1233	Ce-Cu@Alg beads	1219:1233	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	1	71	theme	TBZ	339:341	arg1	pesticide					344:352	dynamic adsorption thiabendazole (TBZ) pesticide	305:352	dynamic adsorption thiabendazole (TBZ) pesticide	305:352	The engineering of a novel biocomposite based on Cerastoderma edule shells doped with copper and alginate (Ce-Cu@Alg) forming hydrogel beads was used for batch and dynamic adsorption thiabendazole (TBZ) pesticide from water.					
36283436	0	72	theme	novel	2:6	arg1	beads					17:21	A novel hydrogel beads	0:21	A novel hydrogel beads based copper-doped Cerastoderma edule shells@Alginate	0:75	A novel hydrogel beads based copper-doped Cerastoderma edule shells@Alginate biocomposite for highly fungicide sorption from aqueous medium.					
36283436	5	73	theme	TBZ	1053:1055	arg1	concentration					1057:1069	initial TBZ concentration	1045:1069	initial TBZ concentration	1045:1069	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	3	74	theme	capacity	835:842	arg1	value					844:848	a maximum adsorption capacity value	814:848	a maximum adsorption capacity value of 21.98 mg/g	814:862	The results of the TBZ batch biosorption by Ce-Cu@Alg composite showed that the Langmuir model was the most adequate to describe the adsorption process, with a maximum adsorption capacity value of 21.98 mg/g.					
36283436	2	75	theme	characterization	414:429	arg1	analysis					504:511	X-ray diffraction analysis	486:511	X-ray diffraction analysis (XRD)	486:517	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	75	theme	characterization	414:429	arg1	thermogravimetric					601:617	thermogravimetric	601:617	thermogravimetric	601:617	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	75	theme	characterization	414:429	arg1	analysis					543:550	Brunauer-Emmett-Teller analysis	520:550	Brunauer-Emmett-Teller analysis (BET)	520:556	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	75	theme	characterization	414:429	arg1	microscopy					468:477	scanning electron microscopy	450:477	scanning electron microscopy (SEM)	450:483	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	75	theme	characterization	414:429	arg1	techniques					431:440	various characterization techniques	406:440	various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA)	406:653	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	75	theme	characterization	414:429	arg1	spectroscopy					581:592	energy dispersive spectroscopy	563:592	energy dispersive spectroscopy (EDS)	563:598	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	75	theme	characterization	414:429	arg1	analysis					636:643	differential analysis	623:643	differential analysis (TGA-DTA)	623:653	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	5	76	theme	6 g/L	1098:1102	arg1	100 ppm					1034:1040	100 ppm	1034:1040	100 ppm of initial TBZ concentration	1034:1069	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	5	76	theme	6 g/L	1098:1102	arg1	dose					1090:1093	a Ce-Cu@Alg beads dose	1072:1093	a Ce-Cu@Alg beads dose of 6 g/L	1072:1102	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	5	76	theme	6 g/L	1098:1102	arg1	time					1118:1121	a contact time	1108:1121	a contact time of 180 min for maximum removal of 83.42%	1108:1162	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	6	77	theme	low	1272:1274	arg1	flow					1285:1288	low effluent flow	1272:1288	low effluent flow	1272:1288	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	2	78	theme	scanning	450:457	arg1	SEM					480:482	SEM	480:482	SEM	480:482	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	2	78	theme	scanning	450:457	arg1	microscopy					468:477	scanning electron microscopy	450:477	scanning electron microscopy (SEM)	450:483	The prepared biosorbent was analyzed by various characterization techniques such as scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller analysis (BET), and energy dispersive spectroscopy (EDS), thermogravimetric and differential analysis (TGA-DTA).					
36283436	6	79	theme	Ce-Cu	1219:1223	arg1	beads					1229:1233	Ce-Cu@Alg beads	1219:1233	Ce-Cu@Alg beads	1219:1233	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	0	80	theme	edule	55:59	arg1	Alginate					68:75	copper-doped Cerastoderma edule shells@Alginate	29:75	copper-doped Cerastoderma edule shells@Alginate	29:75	A novel hydrogel beads based copper-doped Cerastoderma edule shells@Alginate biocomposite for highly fungicide sorption from aqueous medium.					
36283436	5	81	theme	Alg	1080:1082	arg1	beads					1084:1088	Ce-Cu@Alg beads	1074:1088	a Ce-Cu@Alg beads dose of 6 g/L	1072:1102	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	8	82	theme	hybrid	1427:1432	arg1	biocomposite					1434:1445	this new hybrid biocomposite	1418:1445	this new hybrid biocomposite in the industrial sector	1418:1470	This study shows the possibility of using this new hybrid biocomposite in the industrial sector to treat large effluent volumes.					
36283436	6	83	from	sorption	1192:1199	arg1	bed					1212:1214	a fixed bed	1204:1214	a fixed bed of Ce-Cu@Alg beads	1204:1233	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	7	84	theme	kinetic	1362:1368	arg1	data					1370:1373	the kinetic data	1358:1373	the kinetic data	1358:1373	The Thomas model was best fitted to the kinetic data.					
36283436	6	85	from	concentration	1307:1319	arg1	sorption					1192:1199	the TBZ sorption	1184:1199	the TBZ sorption on a fixed bed of Ce-Cu@Alg beads	1184:1233	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	6	85	from	concentration	1307:1319	arg1	effective					1239:1247	effective	1239:1247	effective	1239:1247	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
36283436	5	86	theme	maximum	1138:1144	arg1	removal					1146:1152	maximum removal	1138:1152	maximum removal of 83.42%	1138:1162	The optimal conditions determined by the RSM approach coupled with the CCD design were 100 ppm of initial TBZ concentration, a Ce-Cu@Alg beads dose of 6 g/L and a contact time of 180 min for maximum removal of 83.42%.					
36283436	8	87	theme	industrial	1454:1463	arg1	sector					1465:1470	the industrial sector	1450:1470	the industrial sector	1450:1470	This study shows the possibility of using this new hybrid biocomposite in the industrial sector to treat large effluent volumes.					
36283436	6	88	theme	low	1294:1296	arg1	concentration					1307:1319	low solution concentration	1294:1319	low solution concentration	1294:1319	On the other hand, the TBZ sorption on a fixed bed of Ce-Cu@Alg beads was effective at high column height, low effluent flow and low solution concentration.					
35603878	3	0	theme	electron	753:760	arg1	donor					762:766	an electron donor	750:766	an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes	750:900	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	3	0	theme	electron	753:760	arg1	PVA-Tyr					640:646	PVA-Tyr	640:646	PVA-Tyr	640:646	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	1	1	theme	tissue	314:319	arg1	regeneration					321:332	tissue regeneration	314:332	tissue regeneration	314:332	Biocompatible electrospun fiber comprising bioactive substrates has potential to implant into the wound site as a reliable therapeutic approach in tissue regeneration.					
35603878	1	2	contain	has	231:233	arg2	potential					235:243	potential	235:243	potential	235:243	Biocompatible electrospun fiber comprising bioactive substrates has potential to implant into the wound site as a reliable therapeutic approach in tissue regeneration.					
35603878	1	2	contain	has	231:233	arg1	fiber					193:197	Biocompatible electrospun fiber	167:197	Biocompatible electrospun fiber comprising bioactive substrates	167:229	Biocompatible electrospun fiber comprising bioactive substrates has potential to implant into the wound site as a reliable therapeutic approach in tissue regeneration.					
35603878	2	3	theme	electrospun	341:351	arg1	alcohol					363:369	electrospun polyvinyl alcohol	341:369	electrospun polyvinyl alcohol	341:369	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	8	4	from	proliferation	1581:1593	arg1	fibers					1614:1619	the PVA-Tyr/Col fibers	1598:1619	the PVA-Tyr/Col fibers containing NCs-EGCG	1598:1639	Interestingly, cellular attachment and proliferation on the PVA-Tyr/Col fibers containing NCs-EGCG were higher than control samples.					
35603878	5	5	link	adipose-derived	1055:1069	arg1	cells					1076:1080	adipose-derived stem cells	1055:1080	adipose-derived stem cells	1055:1080	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	4	6	theme	anti-oxidative/inflammatory	915:941	arg1	substrate					943:951	anti-oxidative/inflammatory substrate	915:951	anti-oxidative/inflammatory substrate	915:951	The EGCG as anti-oxidative/inflammatory substrate was encapsulated efficiently in NCs and released in a sustained manner.					
35603878	6	7	theme	typical	1310:1316	arg1	characteristics					1329:1343	all typical absorption characteristics	1306:1343	all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG	1306:1386	Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG.					
35603878	8	8	from	attachment	1566:1575	arg1	fibers					1614:1619	the PVA-Tyr/Col fibers	1598:1619	the PVA-Tyr/Col fibers containing NCs-EGCG	1598:1639	Interestingly, cellular attachment and proliferation on the PVA-Tyr/Col fibers containing NCs-EGCG were higher than control samples.					
35603878	5	9	from	viewpoints	1154:1163	arg1	biocompatibility					1104:1119	appropriate biocompatibility	1092:1119	appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability	1092:1202	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	2	10	theme	patch	617:621	arg1	ability					589:595	in vivo wound healing ability	567:595	in vivo wound healing ability of fabricated wound patch	567:621	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	3	11	theme	groups	819:824	arg1	conjugation					795:805	conjugation	795:805	conjugation of phenolic groups	795:824	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	2	12	theme	alcohol	363:369	arg1	PVA-Tyr					392:398	PVA-Tyr	392:398	PVA-Tyr	392:398	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	2	12	theme	alcohol	363:369	arg1	tyramine					382:389	electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat	341:430	tyramine	382:389	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	1	13	theme	wound	265:269	arg1	site					271:274	the wound site	261:274	the wound site	261:274	Biocompatible electrospun fiber comprising bioactive substrates has potential to implant into the wound site as a reliable therapeutic approach in tissue regeneration.					
35603878	9	14	theme	histological	1679:1690	arg1	analysis					1692:1699	The histological analysis	1675:1699	The histological analysis of hybrid fibrous patch	1675:1723	The histological analysis of hybrid fibrous patch could be suggested the applicability of this structure as suitable skin substitutes to repair injured skin.					
35603878	2	15	theme	fabricated	600:609	arg1	patch					617:621	fabricated wound patch	600:621	fabricated wound patch	600:621	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	3	16	theme	hybrid	844:849	arg1	mat					859:861	hybrid fibrous mat	844:861	hybrid fibrous mat in stable structure and uniform shapes	844:900	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	1	17	from	approach	302:309	arg1	regeneration					321:332	tissue regeneration	314:332	tissue regeneration	314:332	Biocompatible electrospun fiber comprising bioactive substrates has potential to implant into the wound site as a reliable therapeutic approach in tissue regeneration.					
35603878	1	18	theme	Biocompatible	167:179	arg1	fiber					193:197	Biocompatible electrospun fiber	167:197	Biocompatible electrospun fiber comprising bioactive substrates	167:229	Biocompatible electrospun fiber comprising bioactive substrates has potential to implant into the wound site as a reliable therapeutic approach in tissue regeneration.					
35603878	6	19	theme	Col	1360:1362	arg1	NCs					1375:1377	NCs	1375:1377	NCs	1375:1377	Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG.					
35603878	6	19	theme	Col	1360:1362	arg1	EGCG					1383:1386	EGCG	1383:1386	EGCG	1383:1386	Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG.					
35603878	6	19	theme	Col	1360:1362	arg1	characteristics					1329:1343	all typical absorption characteristics	1306:1343	all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG	1306:1386	Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG.					
35603878	5	20	theme	wound	1182:1186	arg1	healing					1188:1194	following wound healing	1172:1194	following wound healing ability	1172:1202	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	3	21	theme	peroxidase	697:706	arg1	reaction					708:715	peroxidase reaction	697:715	peroxidase reaction	697:715	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	3	22	attach	crosslinked	677:687	arg3	presence					720:727	presence	720:727	presence of vaporized H2O2	720:745	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	2	23	theme	in	567:568	arg1	healing					581:587	in vivo wound healing	567:587	in vivo wound healing ability of fabricated wound patch	567:621	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	6	24	theme	PVA-Tyr	1348:1354	arg1	NCs					1375:1377	NCs	1375:1377	NCs	1375:1377	Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG.					
35603878	6	24	theme	PVA-Tyr	1348:1354	arg1	EGCG					1383:1386	EGCG	1383:1386	EGCG	1383:1386	Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG.					
35603878	6	24	theme	PVA-Tyr	1348:1354	arg1	characteristics					1329:1343	all typical absorption characteristics	1306:1343	all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG	1306:1386	Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG.					
35603878	3	25	theme	stable	866:871	arg1	structure					873:881	stable structure	866:881	stable structure	866:881	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	1	26	theme	bioactive	210:218	arg1	substrates					220:229	bioactive substrates	210:229	bioactive substrates	210:229	Biocompatible electrospun fiber comprising bioactive substrates has potential to implant into the wound site as a reliable therapeutic approach in tissue regeneration.					
35603878	3	27	from	mat	859:861	arg1	structure					873:881	stable structure	866:881	stable structure	866:881	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	3	27	from	mat	859:861	arg1	shapes					895:900	uniform shapes	887:900	uniform shapes	887:900	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	0	28	theme	polyvinyl	127:135	arg1	mat					162:164	enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat	101:164	enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat	101:164	Layered dermal reconstitution through epigallocatechin 3-gallate loaded chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat.					
35603878	5	29	theme	excisional	1224:1233	arg1	model					1242:1246	a full-thickness excisional animal model	1207:1246	a full-thickness excisional animal model	1207:1246	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	5	30	theme	stem	1071:1074	arg1	cells					1076:1080	adipose-derived stem cells	1055:1080	adipose-derived stem cells	1055:1080	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	2	31	theme	wound	575:579	arg1	healing					581:587	in vivo wound healing	567:587	in vivo wound healing ability of fabricated wound patch	567:621	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	0	32	theme	fibrous	154:160	arg1	mat					162:164	enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat	101:164	enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat	101:164	Layered dermal reconstitution through epigallocatechin 3-gallate loaded chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat.					
35603878	0	33	theme	Layered	0:6	arg1	reconstitution					15:28	Layered dermal reconstitution	0:28	Layered dermal reconstitution through epigallocatechin 3-gallate	0:63	Layered dermal reconstitution through epigallocatechin 3-gallate loaded chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat.					
35603878	7	34	theme	good	1454:1457	arg1	uniformity					1517:1526	shape uniformity	1511:1526	shape uniformity	1511:1526	The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity.					
35603878	7	34	theme	good	1454:1457	arg1	porosity					1532:1539	porosity	1532:1539	porosity	1532:1539	The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity.					
35603878	7	34	theme	good	1454:1457	arg1	characteristics					1485:1499	good mechanical and structural characteristics	1454:1499	good mechanical and structural characteristics including shape uniformity and porosity	1454:1539	The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity.					
35603878	0	35	theme	epigallocatechin	38:53	arg1	3-gallate					55:63	epigallocatechin 3-gallate	38:63	epigallocatechin 3-gallate	38:63	Layered dermal reconstitution through epigallocatechin 3-gallate loaded chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat.					
35603878	5	36	theme	biochemical	1142:1152	arg1	viewpoints					1154:1163	biophysical and biochemical viewpoints	1126:1163	biophysical and biochemical viewpoints	1126:1163	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	0	37	link	crosslinked	115:125	arg1	mat					162:164	enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat	101:164	enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat	101:164	Layered dermal reconstitution through epigallocatechin 3-gallate loaded chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat.					
35603878	2	38	theme	collagen	405:412	arg1	Col					415:417	Col	415:417	Col	415:417	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	2	38	theme	collagen	405:412	arg1	mat					428:430	electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat	341:430	mat	428:430	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	7	39	theme	perfect	1412:1418	arg1	ratio					1444:1448	the perfect hydrophilic/hydrophobic ratio	1408:1448	the perfect hydrophilic/hydrophobic ratio	1408:1448	The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity.					
35603878	6	40	dep	transform	1257:1265	arg1	infrared					1267:1274	infrared	1267:1274	transform infrared spectroscopy (FTIR)	1257:1294	Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG.					
35603878	9	41	theme	injured	1819:1825	arg1	skin					1827:1830	repair injured skin	1812:1830	repair injured skin	1812:1830	The histological analysis of hybrid fibrous patch could be suggested the applicability of this structure as suitable skin substitutes to repair injured skin.					
35603878	9	42	theme	fibrous	1711:1717	arg1	patch					1719:1723	hybrid fibrous patch	1704:1723	hybrid fibrous patch	1704:1723	The histological analysis of hybrid fibrous patch could be suggested the applicability of this structure as suitable skin substitutes to repair injured skin.					
35603878	3	43	theme	H2O2	742:745	arg1	presence					720:727	presence	720:727	presence of vaporized H2O2	720:745	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	2	44	theme	fibrous	420:426	arg1	Col					415:417	Col	415:417	Col	415:417	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	2	44	theme	fibrous	420:426	arg1	mat					428:430	electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat	341:430	mat	428:430	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	1	45	theme	therapeutic	290:300	arg1	approach					302:309	a reliable therapeutic approach	279:309	a reliable therapeutic approach in tissue regeneration	279:332	Biocompatible electrospun fiber comprising bioactive substrates has potential to implant into the wound site as a reliable therapeutic approach in tissue regeneration.					
35603878	5	46	theme	biophysical	1126:1136	arg1	viewpoints					1154:1163	biophysical and biochemical viewpoints	1126:1163	biophysical and biochemical viewpoints	1126:1163	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	8	47	contain	containing	1621:1630	arg2	NCs-EGCG					1632:1639	NCs-EGCG	1632:1639	NCs-EGCG	1632:1639	Interestingly, cellular attachment and proliferation on the PVA-Tyr/Col fibers containing NCs-EGCG were higher than control samples.					
35603878	8	47	contain	containing	1621:1630	arg1	fibers					1614:1619	the PVA-Tyr/Col fibers	1598:1619	the PVA-Tyr/Col fibers containing NCs-EGCG	1598:1639	Interestingly, cellular attachment and proliferation on the PVA-Tyr/Col fibers containing NCs-EGCG were higher than control samples.					
35603878	2	48	theme	polyvinyl	353:361	arg1	alcohol					363:369	electrospun polyvinyl alcohol	341:369	electrospun polyvinyl alcohol	341:369	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	6	49	theme	absorption	1318:1327	arg1	characteristics					1329:1343	all typical absorption characteristics	1306:1343	all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG	1306:1386	Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG.					
35603878	3	50	theme	phenolic	810:817	arg1	groups					819:824	phenolic groups	810:824	phenolic groups	810:824	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	3	51	theme	synthesized	628:638	arg1	donor					762:766	an electron donor	750:766	an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes	750:900	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	3	51	theme	synthesized	628:638	arg1	PVA-Tyr					640:646	PVA-Tyr	640:646	PVA-Tyr	640:646	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	5	52	from	biocompatibility	1104:1119	arg1	ability					1196:1202	following wound healing ability	1172:1202	following wound healing ability	1172:1202	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	2	53	theme	conjugated	371:380	arg1	PVA-Tyr					392:398	PVA-Tyr	392:398	PVA-Tyr	392:398	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	2	53	theme	conjugated	371:380	arg1	tyramine					382:389	electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat	341:430	tyramine	382:389	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	9	54	theme	repair	1812:1817	arg1	skin					1827:1830	repair injured skin	1812:1830	repair injured skin	1812:1830	The histological analysis of hybrid fibrous patch could be suggested the applicability of this structure as suitable skin substitutes to repair injured skin.					
35603878	5	55	attach	presented	1082:1090	arg1	model					1242:1246	a full-thickness excisional animal model	1207:1246	a full-thickness excisional animal model	1207:1246	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	5	55	attach	presented	1082:1090	arg2	fibers					1036:1041	The hybrid fibers	1025:1041	The hybrid fibers seeded with adipose-derived stem cells	1025:1080	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	4	56	theme	sustained	1007:1015	arg1	manner					1017:1022	a sustained manner	1005:1022	a sustained manner	1005:1022	The EGCG as anti-oxidative/inflammatory substrate was encapsulated efficiently in NCs and released in a sustained manner.					
35603878	2	57	contain	containing	432:441	arg2	nanoparticle					452:463	chitosan nanoparticle	443:463	chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite	443:541	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	2	57	contain	containing	432:441	arg1	PVA-Tyr					392:398	PVA-Tyr	392:398	PVA-Tyr	392:398	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	2	57	contain	containing	432:441	arg1	tyramine					382:389	electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat	341:430	tyramine	382:389	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	5	58	theme	full-thickness	1209:1222	arg1	model					1242:1246	a full-thickness excisional animal model	1207:1246	a full-thickness excisional animal model	1207:1246	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	8	59	theme	control	1658:1664	arg1	samples					1666:1672	control samples	1658:1672	control samples	1658:1672	Interestingly, cellular attachment and proliferation on the PVA-Tyr/Col fibers containing NCs-EGCG were higher than control samples.					
35603878	3	60	theme	fibrous	851:857	arg1	mat					859:861	hybrid fibrous mat	844:861	hybrid fibrous mat in stable structure and uniform shapes	844:900	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	5	61	theme	following	1172:1180	arg1	healing					1188:1194	following wound healing	1172:1194	following wound healing ability	1172:1202	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	1	62	theme	electrospun	181:191	arg1	fiber					193:197	Biocompatible electrospun fiber	167:197	Biocompatible electrospun fiber comprising bioactive substrates	167:229	Biocompatible electrospun fiber comprising bioactive substrates has potential to implant into the wound site as a reliable therapeutic approach in tissue regeneration.					
35603878	3	63	theme	uniform	887:893	arg1	shapes					895:900	uniform shapes	887:900	uniform shapes	887:900	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	5	64	theme	healing	1188:1194	arg1	ability					1196:1202	following wound healing ability	1172:1202	following wound healing ability	1172:1202	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	9	65	theme	hybrid	1704:1709	arg1	patch					1719:1723	hybrid fibrous patch	1704:1723	hybrid fibrous patch	1704:1723	The histological analysis of hybrid fibrous patch could be suggested the applicability of this structure as suitable skin substitutes to repair injured skin.					
35603878	7	66	theme	shape	1511:1515	arg1	uniformity					1517:1526	shape uniformity	1511:1526	shape uniformity	1511:1526	The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity.					
35603878	0	67	theme	crosslinked	115:125	arg1	mat					162:164	enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat	101:164	enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat	101:164	Layered dermal reconstitution through epigallocatechin 3-gallate loaded chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat.					
35603878	9	68	theme	patch	1719:1723	arg1	analysis					1692:1699	The histological analysis	1675:1699	The histological analysis of hybrid fibrous patch	1675:1723	The histological analysis of hybrid fibrous patch could be suggested the applicability of this structure as suitable skin substitutes to repair injured skin.					
35603878	2	69	theme	wound	611:615	arg1	patch					617:621	fabricated wound patch	600:621	fabricated wound patch	600:621	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	0	70	theme	alcohol/collagen	137:152	arg1	mat					162:164	enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat	101:164	enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat	101:164	Layered dermal reconstitution through epigallocatechin 3-gallate loaded chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat.					
35603878	5	71	theme	adipose-derived	1055:1069	arg1	cells					1076:1080	adipose-derived stem cells	1055:1080	adipose-derived stem cells	1055:1080	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	2	72	theme	healing	581:587	arg1	ability					589:595	in vivo wound healing ability	567:595	in vivo wound healing ability of fabricated wound patch	567:621	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	5	73	theme	animal	1235:1240	arg1	model					1242:1246	a full-thickness excisional animal model	1207:1246	a full-thickness excisional animal model	1207:1246	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	7	74	theme	structural	1474:1483	arg1	uniformity					1517:1526	shape uniformity	1511:1526	shape uniformity	1511:1526	The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity.					
35603878	7	74	theme	structural	1474:1483	arg1	porosity					1532:1539	porosity	1532:1539	porosity	1532:1539	The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity.					
35603878	7	74	theme	structural	1474:1483	arg1	characteristics					1485:1499	good mechanical and structural characteristics	1454:1499	good mechanical and structural characteristics including shape uniformity and porosity	1454:1539	The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity.					
35603878	2	75	theme	chitosan	443:450	arg1	nanoparticle					452:463	chitosan nanoparticle	443:463	chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite	443:541	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	0	76	theme	dermal	8:13	arg1	reconstitution					15:28	Layered dermal reconstitution	0:28	Layered dermal reconstitution through epigallocatechin 3-gallate	0:63	Layered dermal reconstitution through epigallocatechin 3-gallate loaded chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat.					
35603878	5	77	theme	hybrid	1029:1034	arg1	fibers					1036:1041	The hybrid fibers	1025:1041	The hybrid fibers seeded with adipose-derived stem cells	1025:1080	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	7	78	theme	mechanical	1459:1468	arg1	uniformity					1517:1526	shape uniformity	1511:1526	shape uniformity	1511:1526	The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity.					
35603878	7	78	theme	mechanical	1459:1468	arg1	porosity					1532:1539	porosity	1532:1539	porosity	1532:1539	The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity.					
35603878	7	78	theme	mechanical	1459:1468	arg1	characteristics					1485:1499	good mechanical and structural characteristics	1454:1499	good mechanical and structural characteristics including shape uniformity and porosity	1454:1539	The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity.					
35603878	9	79	theme	structure	1770:1778	arg1	applicability					1748:1760	the applicability	1744:1760	the applicability of this structure as suitable skin	1744:1795	The histological analysis of hybrid fibrous patch could be suggested the applicability of this structure as suitable skin substitutes to repair injured skin.					
35603878	2	80	theme	epigallocatechin	477:492	arg1	NCs-EGCG					505:512	NCs-EGCG	505:512	NCs-EGCG	505:512	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	2	80	theme	epigallocatechin	477:492	arg1	3-gallate					494:502	epigallocatechin 3-gallate	477:502	epigallocatechin 3-gallate (NCs-EGCG) developed	477:523	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	9	81	theme	suitable	1783:1790	arg1	skin					1792:1795	suitable skin	1783:1795	suitable skin	1783:1795	The histological analysis of hybrid fibrous patch could be suggested the applicability of this structure as suitable skin substitutes to repair injured skin.					
35603878	7	82	theme	hydrophilic/hydrophobic	1420:1442	arg1	ratio					1444:1448	the perfect hydrophilic/hydrophobic ratio	1408:1448	the perfect hydrophilic/hydrophobic ratio	1408:1448	The results showed the perfect hydrophilic/hydrophobic ratio and good mechanical and structural characteristics including shape uniformity and porosity.					
35603878	8	83	theme	cellular	1557:1564	arg1	attachment					1566:1575	cellular attachment	1557:1575	cellular attachment	1557:1575	Interestingly, cellular attachment and proliferation on the PVA-Tyr/Col fibers containing NCs-EGCG were higher than control samples.					
35603878	0	84	theme	chitosan	72:79	arg1	nanoparticle					81:92	chitosan nanoparticle	72:92	chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat	72:164	Layered dermal reconstitution through epigallocatechin 3-gallate loaded chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat.					
35603878	2	85	dep	in	567:568	arg1	vivo					570:573	vivo	570:573	vivo	570:573	Here, electrospun polyvinyl alcohol conjugated tyramine (PVA-Tyr) and collagen (Col) fibrous mat containing chitosan nanoparticle loaded with epigallocatechin 3-gallate (NCs-EGCG) developed and the composite was applied to evaluate in vivo wound healing ability of fabricated wound patch.					
35603878	5	86	theme	appropriate	1092:1102	arg1	biocompatibility					1104:1119	appropriate biocompatibility	1092:1119	appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability	1092:1202	The hybrid fibers seeded with adipose-derived stem cells presented appropriate biocompatibility from biophysical and biochemical viewpoints and in following wound healing ability in a full-thickness excisional animal model.					
35603878	3	87	theme	vaporized	732:740	arg1	H2O2					742:745	vaporized H2O2	732:745	vaporized H2O2	732:745	The synthesized PVA-Tyr and Col were electrospun and crosslinked through peroxidase reaction in presence of vaporized H2O2 as an electron donor which covalently proceeded conjugation of phenolic groups and could develop hybrid fibrous mat in stable structure and uniform shapes.					
35603878	6	88	dep	Fourier	1249:1255	arg1	transform					1257:1265	transform	1257:1265	transform infrared spectroscopy (FTIR)	1257:1294	Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PVA-Tyr and Col as well as NCs and EGCG.					
35603878	1	89	theme	reliable	281:288	arg1	approach					302:309	a reliable therapeutic approach	279:309	a reliable therapeutic approach in tissue regeneration	279:332	Biocompatible electrospun fiber comprising bioactive substrates has potential to implant into the wound site as a reliable therapeutic approach in tissue regeneration.					
35603878	8	90	theme	PVA-Tyr/Col	1602:1612	arg1	fibers					1614:1619	the PVA-Tyr/Col fibers	1598:1619	the PVA-Tyr/Col fibers containing NCs-EGCG	1598:1639	Interestingly, cellular attachment and proliferation on the PVA-Tyr/Col fibers containing NCs-EGCG were higher than control samples.					
37376765	10	0	theme	administered	1738:1749	arg1	PHT					1751:1753	orally administered PHT	1731:1753	orally administered PHT	1731:1753	The composition of ENs may affect the absorption of PHT differently, and these findings would aid in the selection of ENs for orally administered PHT.					
37376765	2	1	theme	degraded	557:564	arg1	guar-gum					566:573	degraded guar-gum	557:573	degraded guar-gum	557:573	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	4	2	theme	artificial	1029:1038	arg1	P-Casein					1058:1065	P-Casein	1058:1065	P-Casein	1058:1065	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	4	2	theme	artificial	1029:1038	arg1	juice					1051:1055	artificial pancreatic juice	1029:1055	artificial pancreatic juice (P-Casein)	1029:1066	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	3	3	dep	juice	790:794	arg1	mg/mL					846:850	10 mg/mL	843:850	10 mg/mL	843:850	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	3	4	theme	gastric	782:788	arg1	juice					790:794	artificial gastric juice	771:794	artificial gastric juice (G-Soy)	771:802	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	3	4	theme	gastric	782:788	arg1	G-Soy					797:801	G-Soy	797:801	G-Soy	797:801	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	3	5	theme	soy	746:748	arg1	protein					750:756	soy protein	746:756	soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL	746:850	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	2	6	theme	simulated	480:488	arg1	casein					516:521	simulated gastrointestinal digested casein	480:521	simulated gastrointestinal digested casein	480:521	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	0	7	from	Study	0:4	arg1	components					26:35	enteral nutrient components	9:35	enteral nutrient components causing	9:43	Study on enteral nutrient components causing decreased gastric phenytoin absorption.					
37376765	9	8	theme	tight	1588:1592	arg1	junctions					1594:1602	tight junctions	1588:1602	tight junctions	1588:1602	However, digested casein decreased PHT absorption by reducing the strength of tight junctions.					
37376765	2	9	dep	METHODS	335:341	arg1	measured					662:669	measured	662:669	measured the solution's properties	662:695	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	2	9	dep	METHODS	335:341	arg1	measured					346:353	measured	346:353	measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs	346:650	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	1	10	theme	antiepileptic	259:271	arg1	phenytoin					239:247	phenytoin	239:247	phenytoin (PHT)	239:253	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	1	10	theme	antiepileptic	259:271	arg1	drug					273:276	an antiepileptic drug	256:276	an antiepileptic drug	256:276	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	6	11	theme	casein	1203:1208	arg1	mg/mL					1213:1217	casein 40 mg/mL	1203:1217	casein 40 mg/mL	1203:1217	Furthermore, casein 40 mg/mL and dextrin 100 mg/mL have high viscosity.					
37376765	4	12	theme	artificial	990:999	arg1	juice					1009:1013	artificial gastric juice	990:1013	artificial gastric juice (G-casein)	990:1024	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	4	12	theme	artificial	990:999	arg1	G-casein					1016:1023	G-casein	1016:1023	G-casein	1016:1023	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	3	13	theme	artificial	807:816	arg1	P-Soy					836:840	P-Soy	836:840	P-Soy	836:840	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	3	13	theme	artificial	807:816	arg1	juice					829:833	artificial pancreatic juice	807:833	artificial pancreatic juice (P-Soy)	807:841	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	8	14	theme	gastric	1483:1489	arg1	absorption					1491:1500	the gastric absorption	1479:1500	the gastric absorption of PHT	1479:1507	CONCLUSION Casein, digested soy protein, and dextrin decreased the gastric absorption of PHT.					
37376765	3	15	dep	RESULTS	698:704	arg1	demonstrated					709:720	demonstrated	709:720	demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control	709:952	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	4	16	theme	PHT	1117:1119	arg1	rate					1109:1112	the permeability rate	1092:1112	the permeability rate of PHT	1092:1119	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	2	17	theme	permeability	359:370	arg1	rate					372:375	the permeability rate	355:375	the permeability rate of PHT	355:382	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	2	18	theme	soy	467:469	arg1	protein					471:477	soy protein	467:477	soy protein	467:477	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	2	19	theme	indigestible	576:587	arg1	dextrin					589:595	indigestible dextrin	576:595	indigestible dextrin	576:595	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	2	20	from	abundant	632:639	arg1	ENs					648:650	the ENs	644:650	the ENs	644:650	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	3	21	theme	PHT	926:928	arg1	rate					918:921	the permeability rate	901:921	the permeability rate of PHT	901:928	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	1	22	theme	plasma	191:196	arg1	concentrations					198:211	plasma concentrations	191:211	plasma concentrations	191:211	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	3	23	theme	permeability	905:916	arg1	rate					918:921	the permeability rate	901:921	the permeability rate of PHT	901:928	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	10	24	theme	ENs	1723:1725	arg1	selection					1710:1718	the selection	1706:1718	the selection of ENs for orally administered PHT	1706:1753	The composition of ENs may affect the absorption of PHT differently, and these findings would aid in the selection of ENs for orally administered PHT.					
37376765	0	25	theme	nutrient	17:24	arg1	components					26:35	enteral nutrient components	9:35	enteral nutrient components causing	9:43	Study on enteral nutrient components causing decreased gastric phenytoin absorption.					
37376765	4	26	theme	permeability	1096:1107	arg1	rate					1109:1112	the permeability rate	1092:1112	the permeability rate of PHT	1092:1119	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	9	27	theme	PHT	1545:1547	arg1	absorption					1549:1558	PHT absorption	1545:1558	PHT absorption	1545:1558	However, digested casein decreased PHT absorption by reducing the strength of tight junctions.					
37376765	5	28	theme	casein	1165:1170	arg1	mg/mL					1175:1179	casein 40 mg/mL	1165:1179	casein 40 mg/mL	1165:1179	We also found that the PHT binding rate to casein 40 mg/mL was 90%.					
37376765	10	29	theme	ENs	1624:1626	arg1	composition					1609:1619	The composition	1605:1619	The composition of ENs	1605:1626	The composition of ENs may affect the absorption of PHT differently, and these findings would aid in the selection of ENs for orally administered PHT.					
37376765	4	30	theme	casein	968:973	arg1	treatment					975:983	casein treatment	968:983	casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein)	968:1066	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	2	31	theme	Caco-2	392:397	arg1	monolayer					404:412	a Caco-2 cell monolayer	390:412	a Caco-2 cell monolayer	390:412	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	2	31	theme	Caco-2	392:397	arg1	model					447:451	a human intestinal absorption model	417:451	a human intestinal absorption model	417:451	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	2	32	theme	cell	399:402	arg1	monolayer					404:412	a Caco-2 cell monolayer	390:412	a Caco-2 cell monolayer	390:412	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	2	32	theme	cell	399:402	arg1	model					447:451	a human intestinal absorption model	417:451	a human intestinal absorption model	417:451	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	8	33	theme	CONCLUSION	1416:1425	arg1	Casein					1427:1432	CONCLUSION Casein	1416:1432	CONCLUSION Casein	1416:1432	CONCLUSION Casein, digested soy protein, and dextrin decreased the gastric absorption of PHT.					
37376765	6	34	theme	high	1246:1249	arg1	viscosity					1251:1259	high viscosity	1246:1259	high viscosity	1246:1259	Furthermore, casein 40 mg/mL and dextrin 100 mg/mL have high viscosity.					
37376765	2	35	theme	intestinal	425:434	arg1	monolayer					404:412	a Caco-2 cell monolayer	390:412	a Caco-2 cell monolayer	390:412	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	2	35	theme	intestinal	425:434	arg1	model					447:451	a human intestinal absorption model	417:451	a human intestinal absorption model	417:451	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	1	36	theme	gastric	217:223	arg1	absorption					225:234	gastric absorption	217:234	gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats	217:285	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	3	37	theme	pancreatic	818:827	arg1	P-Soy					836:840	P-Soy	836:840	P-Soy	836:840	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	3	37	theme	pancreatic	818:827	arg1	juice					829:833	artificial pancreatic juice	807:833	artificial pancreatic juice (P-Soy)	807:841	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	2	38	theme	digested	507:514	arg1	casein					516:521	simulated gastrointestinal digested casein	480:521	simulated gastrointestinal digested casein	480:521	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	10	39	theme	PHT	1657:1659	arg1	absorption					1643:1652	the absorption	1639:1652	the absorption of PHT	1639:1659	The composition of ENs may affect the absorption of PHT differently, and these findings would aid in the selection of ENs for orally administered PHT.					
37376765	6	40	contain	have	1241:1244	arg1	mg/mL					1235:1239	dextrin 100 mg/mL	1223:1239	dextrin 100 mg/mL	1223:1239	Furthermore, casein 40 mg/mL and dextrin 100 mg/mL have high viscosity.					
37376765	6	40	contain	have	1241:1244	arg2	viscosity					1251:1259	high viscosity	1246:1259	high viscosity	1246:1259	Furthermore, casein 40 mg/mL and dextrin 100 mg/mL have high viscosity.					
37376765	6	40	contain	have	1241:1244	arg1	mg/mL					1213:1217	casein 40 mg/mL	1203:1217	casein 40 mg/mL	1203:1217	Furthermore, casein 40 mg/mL and dextrin 100 mg/mL have high viscosity.					
37376765	2	41	theme	absorption	436:445	arg1	monolayer					404:412	a Caco-2 cell monolayer	390:412	a Caco-2 cell monolayer	390:412	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	2	41	theme	absorption	436:445	arg1	model					447:451	a human intestinal absorption model	417:451	a human intestinal absorption model	417:451	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	2	42	theme	gastrointestinal	490:505	arg1	casein					516:521	simulated gastrointestinal digested casein	480:521	simulated gastrointestinal digested casein	480:521	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	1	43	from	absorption	225:234	arg1	rats					282:285	rats	282:285	rats	282:285	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	2	44	theme	PHT	380:382	arg1	rate					372:375	the permeability rate	355:375	the permeability rate of PHT	355:382	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	0	45	theme	gastric	55:61	arg1	absorption					73:82	gastric phenytoin absorption	55:82	gastric phenytoin absorption	55:82	Study on enteral nutrient components causing decreased gastric phenytoin absorption.					
37376765	4	46	theme	gastric	1001:1007	arg1	juice					1009:1013	artificial gastric juice	990:1013	artificial gastric juice (G-casein)	990:1024	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	4	46	theme	gastric	1001:1007	arg1	G-casein					1016:1023	G-casein	1016:1023	G-casein	1016:1023	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	6	47	theme	dextrin	1223:1229	arg1	mg/mL					1235:1239	dextrin 100 mg/mL	1223:1239	dextrin 100 mg/mL	1223:1239	Furthermore, casein 40 mg/mL and dextrin 100 mg/mL have high viscosity.					
37376765	2	48	theme	human	419:423	arg1	monolayer					404:412	a Caco-2 cell monolayer	390:412	a Caco-2 cell monolayer	390:412	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	2	48	theme	human	419:423	arg1	model					447:451	a human intestinal absorption model	417:451	a human intestinal absorption model	417:451	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	8	49	theme	soy	1444:1446	arg1	protein					1448:1454	digested soy protein	1435:1454	digested soy protein	1435:1454	CONCLUSION Casein, digested soy protein, and dextrin decreased the gastric absorption of PHT.					
37376765	7	50	theme	cell	1364:1367	arg1	monolayers					1369:1378	Caco-2 cell monolayers	1357:1378	Caco-2 cell monolayers	1357:1378	Moreover, G- and P-Casein significantly decreased the transepithelial electrical resistance of Caco-2 cell monolayers compared to casein and the control.					
37376765	9	51	theme	digested	1519:1526	arg1	casein					1528:1533	digested casein	1519:1533	digested casein	1519:1533	However, digested casein decreased PHT absorption by reducing the strength of tight junctions.					
37376765	1	52	dep	BACKGROUND	85:94	arg1	revealed					111:118	revealed	111:118	revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.	111:333	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	8	53	theme	PHT	1505:1507	arg1	absorption					1491:1500	the gastric absorption	1479:1500	the gastric absorption of PHT	1479:1507	CONCLUSION Casein, digested soy protein, and dextrin decreased the gastric absorption of PHT.					
37376765	8	54	theme	digested	1435:1442	arg1	protein					1448:1454	digested soy protein	1435:1454	digested soy protein	1435:1454	CONCLUSION Casein, digested soy protein, and dextrin decreased the gastric absorption of PHT.					
37376765	4	55	with	treatment	975:983	arg1	juice					1009:1013	artificial gastric juice	990:1013	artificial gastric juice (G-casein)	990:1024	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	4	55	with	treatment	975:983	arg1	P-Casein					1058:1065	P-Casein	1058:1065	P-Casein	1058:1065	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	4	55	with	treatment	975:983	arg1	juice					1051:1055	artificial pancreatic juice	1029:1055	artificial pancreatic juice (P-Casein)	1029:1066	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	4	55	with	treatment	975:983	arg1	G-casein					1016:1023	G-casein	1016:1023	G-casein	1016:1023	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	7	56	theme	monolayers	1369:1378	arg1	resistance					1343:1352	the transepithelial electrical resistance	1312:1352	the transepithelial electrical resistance of Caco-2 cell monolayers	1312:1378	Moreover, G- and P-Casein significantly decreased the transepithelial electrical resistance of Caco-2 cell monolayers compared to casein and the control.					
37376765	2	57	from	ENs	648:650	arg1	abundant					632:639	abundant	632:639	abundant	632:639	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	7	58	theme	transepithelial	1316:1330	arg1	resistance					1343:1352	the transepithelial electrical resistance	1312:1352	the transepithelial electrical resistance of Caco-2 cell monolayers	1312:1378	Moreover, G- and P-Casein significantly decreased the transepithelial electrical resistance of Caco-2 cell monolayers compared to casein and the control.					
37376765	3	59	theme	artificial	771:780	arg1	juice					790:794	artificial gastric juice	771:794	artificial gastric juice (G-Soy)	771:802	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	3	59	theme	artificial	771:780	arg1	G-Soy					797:801	G-Soy	797:801	G-Soy	797:801	RESULTS We demonstrated that casein (40 mg/mL), soy protein treated with artificial gastric juice (G-Soy) or artificial pancreatic juice (P-Soy) 10 mg/mL, and dextrin (100 mg/mL) significantly decreased the permeability rate of PHT compared to the control.					
37376765	1	60	theme	phenytoin	239:247	arg1	concentrations					198:211	plasma concentrations	191:211	plasma concentrations	191:211	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	1	60	theme	phenytoin	239:247	arg1	absorption					225:234	gastric absorption	217:234	gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats	217:285	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	2	61	theme	soy	526:528	arg1	protein					530:536	soy protein	526:536	soy protein	526:536	METHODS We measured the permeability rate of PHT using a Caco-2 cell monolayer as a human intestinal absorption model, with casein, soy protein, simulated gastrointestinal digested casein or soy protein, dextrin, sucrose, degraded guar-gum, indigestible dextrin, calcium, and magnesium, which are abundant in the ENs, and also measured the solution's properties.					
37376765	1	62	theme	particular	146:155	arg1	nutrients					165:173	particular enteral nutrients	146:173	particular enteral nutrients (ENs)	146:179	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	1	62	theme	particular	146:155	arg1	ENs					176:178	ENs	176:178	ENs	176:178	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	5	63	theme	binding	1149:1155	arg1	%					1187:1187	90%	1185:1187	90%	1185:1187	We also found that the PHT binding rate to casein 40 mg/mL was 90%.					
37376765	5	63	theme	binding	1149:1155	arg1	rate					1157:1160	the PHT binding rate	1141:1160	the PHT binding rate to casein 40 mg/mL	1141:1179	We also found that the PHT binding rate to casein 40 mg/mL was 90%.					
37376765	9	64	theme	junctions	1594:1602	arg1	strength					1576:1583	the strength	1572:1583	the strength of tight junctions	1572:1602	However, digested casein decreased PHT absorption by reducing the strength of tight junctions.					
37376765	7	65	theme	Caco-2	1357:1362	arg1	monolayers					1369:1378	Caco-2 cell monolayers	1357:1378	Caco-2 cell monolayers	1357:1378	Moreover, G- and P-Casein significantly decreased the transepithelial electrical resistance of Caco-2 cell monolayers compared to casein and the control.					
37376765	1	66	from	concentrations	198:211	arg1	rats					282:285	rats	282:285	rats	282:285	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	1	67	theme	enteral	157:163	arg1	nutrients					165:173	particular enteral nutrients	146:173	particular enteral nutrients (ENs)	146:179	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	1	67	theme	enteral	157:163	arg1	ENs					176:178	ENs	176:178	ENs	176:178	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
37376765	5	68	theme	PHT	1145:1147	arg1	%					1187:1187	90%	1185:1187	90%	1185:1187	We also found that the PHT binding rate to casein 40 mg/mL was 90%.					
37376765	5	68	theme	PHT	1145:1147	arg1	rate					1157:1160	the PHT binding rate	1141:1160	the PHT binding rate to casein 40 mg/mL	1141:1179	We also found that the PHT binding rate to casein 40 mg/mL was 90%.					
37376765	0	69	theme	phenytoin	63:71	arg1	absorption					73:82	gastric phenytoin absorption	55:82	gastric phenytoin absorption	55:82	Study on enteral nutrient components causing decreased gastric phenytoin absorption.					
37376765	7	70	theme	electrical	1332:1341	arg1	resistance					1343:1352	the transepithelial electrical resistance	1312:1352	the transepithelial electrical resistance of Caco-2 cell monolayers	1312:1378	Moreover, G- and P-Casein significantly decreased the transepithelial electrical resistance of Caco-2 cell monolayers compared to casein and the control.					
37376765	0	71	theme	enteral	9:15	arg1	components					26:35	enteral nutrient components	9:35	enteral nutrient components causing	9:43	Study on enteral nutrient components causing decreased gastric phenytoin absorption.					
37376765	4	72	theme	pancreatic	1040:1049	arg1	P-Casein					1058:1065	P-Casein	1058:1065	P-Casein	1058:1065	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	4	72	theme	pancreatic	1040:1049	arg1	juice					1051:1055	artificial pancreatic juice	1029:1055	artificial pancreatic juice (P-Casein)	1029:1066	In contrast, casein treatment with artificial gastric juice (G-casein) or artificial pancreatic juice (P-Casein) significantly increased the permeability rate of PHT.					
37376765	1	73	theme	nutrients	165:173	arg1	co-administration					125:141	co-administration	125:141	co-administration of particular enteral nutrients (ENs)	125:179	BACKGROUND Previously, we revealed that co-administration of particular enteral nutrients (ENs) decreases plasma concentrations and gastric absorption of phenytoin (PHT), an antiepileptic drug, in rats; however, the mechanism has not been clarified.					
35788780	6	0	theme	related	1278:1284	arg1	genes					1286:1290	denitrification related genes	1262:1290	denitrification related genes	1262:1290	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	2	1	theme	material	582:589	arg1	application					591:601	organic material application	574:601	organic material application	574:601	The relative abundance of bacterial and viral groups was strongly affected by organic material application, whereas that of the archaeal, protist and fungal groups was less affected.					
35788780	5	2	from	Myoviridae	1135:1144	arg1	soil					1163:1166	the unamended soil	1149:1166	the unamended soil	1149:1166	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	1	3	theme	mays	446:449	arg1	L.					451:452	Zea mays L.	442:452	Zea mays L.	442:452	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	1	3	theme	mays	446:449	arg1	plants					434:439	young maize plants	422:439	young maize plants (Zea mays L.)	422:453	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	8	4	theme	soil	1695:1698	arg1	fungi					1700:1704	soil fungi	1695:1704	soil fungi	1695:1704	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	7	5	theme	bacterial	1461:1469	arg1	groups					1471:1476	Copiotrophic life-style bacterial groups	1437:1476	Copiotrophic life-style bacterial groups	1437:1476	Copiotrophic life-style bacterial groups were enriched in the amended soil, whereas oligotrophic life-style bacterial groups in the unamended soil.					
35788780	6	6	theme	C	1246:1246	arg1	compounds					1248:1256	complex C compounds	1238:1256	complex C compounds	1238:1256	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	6	7	dep	enriched	1297:1304	arg1	whereas					1372:1378	whereas	1372:1378	whereas	1372:1378	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	7	8	theme	Copiotrophic	1437:1448	arg1	groups					1471:1476	Copiotrophic life-style bacterial groups	1437:1476	Copiotrophic life-style bacterial groups	1437:1476	Copiotrophic life-style bacterial groups were enriched in the amended soil, whereas oligotrophic life-style bacterial groups in the unamended soil.					
35788780	5	9	from	soil	1163:1166	arg1	members					1067:1073	members	1067:1073	members of Siphoviridae enriched in the amended soil	1067:1118	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	5	9	from	soil	1163:1166	arg1	members					1124:1130	members	1124:1130	members of Myoviridae in the unamended soil	1124:1166	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	2	10	theme	groups	542:547	arg1	abundance					509:517	The relative abundance	496:517	The relative abundance of bacterial and viral groups	496:547	The relative abundance of bacterial and viral groups was strongly affected by organic material application, whereas that of the archaeal, protist and fungal groups was less affected.					
35788780	9	11	theme	plant	1925:1929	arg1	soil					1939:1942	the maize plant amended soil	1915:1942	the maize plant amended soil	1915:1942	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	6	12	theme	plant	1325:1329	arg1	soil					1339:1342	the young maize plant amended soil	1309:1342	the young maize plant amended soil	1309:1342	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	6	13	theme	young	1313:1317	arg1	soil					1339:1342	the young maize plant amended soil	1309:1342	the young maize plant amended soil	1309:1342	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	1	14	theme	young	422:426	arg1	L.					451:452	Zea mays L.	442:452	Zea mays L.	442:452	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	1	14	theme	young	422:426	arg1	plants					434:439	young maize plants	422:439	young maize plants (Zea mays L.)	422:453	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	6	15	theme	related	1203:1209	arg1	traits					1196:1201	More specialized metabolic traits	1169:1201	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes	1169:1290	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	9	16	theme	unamended	1956:1964	arg1	soil					1966:1969	the unamended soil	1952:1969	the unamended soil	1952:1969	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	5	17	theme	viral	1048:1052	arg1	family					1054:1059	the dominant viral family	1035:1059	the dominant viral family	1035:1059	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	5	17	theme	viral	1048:1052	arg1	Caudovirales					1018:1029	Caudovirales	1018:1029	Caudovirales	1018:1029	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	3	18	theme	growing	818:824	arg1	metabolism					865:874	slow growing oligotrophic and chemolithoautotrophic metabolism	813:874	slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea	813:902	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria) were enriched in the amended soil, whereas the groups with slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea were greater in the unamended than in the amended soil.					
35788780	4	19	theme	soil	964:967	arg1	structure					975:983	The soil viral structure	960:983	The soil viral structure	960:983	The soil viral structure and richness were also affected.					
35788780	6	20	theme	metabolic	1186:1194	arg1	traits					1196:1201	More specialized metabolic traits	1169:1201	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes	1169:1290	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	3	21	theme	amended	775:781	arg1	soil					783:786	the amended soil	771:786	the amended soil	771:786	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria) were enriched in the amended soil, whereas the groups with slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea were greater in the unamended than in the amended soil.					
35788780	0	22	theme	microbiome	53:62	arg1	composition					64:74	the microbiome composition	49:74	the microbiome composition	49:74	Application of young maize plant residues alters the microbiome composition and its functioning in a soil under conservation agriculture: a metagenomics study.					
35788780	6	23	from	soil	1366:1369	arg1	enriched					1297:1304	enriched	1297:1304	enriched	1297:1304	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	9	24	theme	Metabolic	1750:1758	arg1	functionality					1760:1772	Metabolic functionality	1750:1772	Metabolic functionality	1750:1772	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	6	25	theme	unamended	1356:1364	arg1	soil					1366:1369	the unamended soil	1352:1369	the unamended soil	1352:1369	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	3	26	dep	Betaproteobacteria	734:751	arg1	e.g.					728:731	e.g.	728:731	e.g.	728:731	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria) were enriched in the amended soil, whereas the groups with slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea were greater in the unamended than in the amended soil.					
35788780	1	27	theme	maize	428:432	arg1	L.					451:452	Zea mays L.	442:452	Zea mays L.	442:452	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	1	27	theme	maize	428:432	arg1	plants					434:439	young maize plants	422:439	young maize plants (Zea mays L.)	422:453	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	3	28	dep	growing	818:824	arg1	slow					813:816	slow	813:816	slow	813:816	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria) were enriched in the amended soil, whereas the groups with slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea were greater in the unamended than in the amended soil.					
35788780	7	29	theme	unamended	1569:1577	arg1	soil					1579:1582	the unamended soil	1565:1582	the unamended soil	1565:1582	Copiotrophic life-style bacterial groups were enriched in the amended soil, whereas oligotrophic life-style bacterial groups in the unamended soil.					
35788780	2	30	theme	protist	634:640	arg1	groups					653:658	the archaeal, protist and fungal groups	620:658	the archaeal, protist and fungal groups	620:658	The relative abundance of bacterial and viral groups was strongly affected by organic material application, whereas that of the archaeal, protist and fungal groups was less affected.					
35788780	5	31	theme	Siphoviridae	1078:1089	arg1	members					1067:1073	members	1067:1073	members of Siphoviridae enriched in the amended soil	1067:1118	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	5	31	theme	Siphoviridae	1078:1089	arg1	members					1124:1130	members	1124:1130	members of Myoviridae in the unamended soil	1124:1166	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	2	32	theme	relative	500:507	arg1	abundance					509:517	The relative abundance	496:517	The relative abundance of bacterial and viral groups	496:547	The relative abundance of bacterial and viral groups was strongly affected by organic material application, whereas that of the archaeal, protist and fungal groups was less affected.					
35788780	2	33	theme	archaeal	624:631	arg1	groups					653:658	the archaeal, protist and fungal groups	620:658	the archaeal, protist and fungal groups	620:658	The relative abundance of bacterial and viral groups was strongly affected by organic material application, whereas that of the archaeal, protist and fungal groups was less affected.					
35788780	5	34	with	family	1054:1059	arg1	members					1067:1073	members	1067:1073	members of Siphoviridae enriched in the amended soil	1067:1118	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	5	34	with	family	1054:1059	arg1	members					1124:1130	members	1124:1130	members of Myoviridae in the unamended soil	1124:1166	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	5	35	theme	unamended	1153:1161	arg1	soil					1163:1166	the unamended soil	1149:1166	the unamended soil	1149:1166	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	2	36	theme	fungal	646:651	arg1	groups					653:658	the archaeal, protist and fungal groups	620:658	the archaeal, protist and fungal groups	620:658	The relative abundance of bacterial and viral groups was strongly affected by organic material application, whereas that of the archaeal, protist and fungal groups was less affected.					
35788780	0	37	theme	young	15:19	arg1	residues					33:40	young maize plant residues	15:40	young maize plant residues	15:40	Application of young maize plant residues alters the microbiome composition and its functioning in a soil under conservation agriculture: a metagenomics study.					
35788780	1	38	theme	metagenomic	286:296	arg1	sequencing					298:307	shotgun metagenomic sequencing	278:307	shotgun metagenomic sequencing	278:307	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	7	39	theme	life-style	1534:1543	arg1	groups					1555:1560	oligotrophic life-style bacterial groups	1521:1560	oligotrophic life-style bacterial groups in the unamended soil	1521:1582	Copiotrophic life-style bacterial groups were enriched in the amended soil, whereas oligotrophic life-style bacterial groups in the unamended soil.					
35788780	9	40	theme	material	1817:1824	arg1	application					1794:1804	the application	1790:1804	the application of organic material	1790:1824	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	5	41	from	members	1124:1130	arg1	soil					1163:1166	the unamended soil	1149:1166	the unamended soil	1149:1166	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	6	42	from	enriched	1297:1304	arg1	soil					1366:1369	the unamended soil	1352:1369	the unamended soil	1352:1369	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	6	42	from	enriched	1297:1304	arg1	soil					1339:1342	the young maize plant amended soil	1309:1342	the young maize plant amended soil	1309:1342	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	0	43	theme	plant	27:31	arg1	residues					33:40	young maize plant residues	15:40	young maize plant residues	15:40	Application of young maize plant residues alters the microbiome composition and its functioning in a soil under conservation agriculture: a metagenomics study.					
35788780	0	44	dep	alters	42:47	arg1	study					153:157	a metagenomics study	138:157	a metagenomics study	138:157	Application of young maize plant residues alters the microbiome composition and its functioning in a soil under conservation agriculture: a metagenomics study.					
35788780	6	45	theme	nitrification	1380:1392	arg1	genes					1402:1406	nitrification related genes	1380:1406	nitrification related genes	1380:1406	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	5	46	theme	amended	1107:1113	arg1	soil					1115:1118	the amended soil	1103:1118	the amended soil	1103:1118	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	8	47	theme	Many	1585:1588	arg1	phylotypes					1610:1619	Many bacterial and viral phylotypes	1585:1619	Many bacterial and viral phylotypes	1585:1619	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	1	48	theme	organic	208:214	arg1	material					216:223	organic material	208:223	organic material	208:223	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	9	49	theme	genes	1855:1859	arg1	abundance					1842:1850	the relative abundance	1829:1850	the relative abundance of genes involved in the denitrification process	1829:1899	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	9	49	theme	genes	1855:1859	arg1	higher					1905:1910	higher	1905:1910	higher	1905:1910	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	8	50	theme	maize	1663:1667	arg1	plants					1669:1674	young maize plants	1657:1674	young maize plants	1657:1674	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	1	51	theme	microbial	237:245	arg1	populations					247:257	soil microbial populations	232:257	soil microbial populations	232:257	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	1	52	used	used	313:316	arg2	sequencing					298:307	shotgun metagenomic sequencing	278:307	shotgun metagenomic sequencing	278:307	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	9	53	theme	denitrification	1877:1891	arg1	process					1893:1899	the denitrification process	1873:1899	the denitrification process	1873:1899	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	6	54	theme	denitrification	1262:1276	arg1	genes					1286:1290	denitrification related genes	1262:1290	denitrification related genes	1262:1290	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	1	55	theme	Zea	442:444	arg1	L.					451:452	Zea mays L.	442:452	Zea mays L.	442:452	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	1	55	theme	Zea	442:444	arg1	plants					434:439	young maize plants	422:439	young maize plants (Zea mays L.)	422:453	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	3	56	with	degraders	689:697	arg1	lifestyle					717:725	copiotrophic lifestyle	704:725	copiotrophic lifestyle (e.g., Betaproteobacteria)	704:752	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria) were enriched in the amended soil, whereas the groups with slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea were greater in the unamended than in the amended soil.					
35788780	7	57	theme	life-style	1450:1459	arg1	groups					1471:1476	Copiotrophic life-style bacterial groups	1437:1476	Copiotrophic life-style bacterial groups	1437:1476	Copiotrophic life-style bacterial groups were enriched in the amended soil, whereas oligotrophic life-style bacterial groups in the unamended soil.					
35788780	2	58	theme	organic	574:580	arg1	application					591:601	organic material application	574:601	organic material application	574:601	The relative abundance of bacterial and viral groups was strongly affected by organic material application, whereas that of the archaeal, protist and fungal groups was less affected.					
35788780	6	59	theme	compounds	1248:1256	arg1	genes					1286:1290	denitrification related genes	1262:1290	denitrification related genes	1262:1290	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	6	59	theme	compounds	1248:1256	arg1	degradation					1223:1233	the degradation	1219:1233	the degradation of complex C compounds	1219:1256	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	3	60	theme	copiotrophic	704:715	arg1	lifestyle					717:725	copiotrophic lifestyle	704:725	copiotrophic lifestyle (e.g., Betaproteobacteria)	704:752	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria) were enriched in the amended soil, whereas the groups with slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea were greater in the unamended than in the amended soil.					
35788780	6	61	theme	complex	1238:1244	arg1	compounds					1248:1256	complex C compounds	1238:1256	complex C compounds	1238:1256	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	8	62	theme	fungi	1700:1704	arg1	smaller					1741:1747	smaller	1741:1747	smaller	1741:1747	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	8	62	theme	fungi	1700:1704	arg1	number					1685:1690	the number	1681:1690	the number of soil fungi, archaea and protists affected	1681:1735	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	9	63	theme	maize	1919:1923	arg1	soil					1939:1942	the maize plant amended soil	1915:1942	the maize plant amended soil	1915:1942	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	6	64	theme	amended	1331:1337	arg1	soil					1339:1342	the young maize plant amended soil	1309:1342	the young maize plant amended soil	1309:1342	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	1	65	theme	laboratory	460:469	arg1	experiment					471:480	a laboratory experiment	458:480	a laboratory experiment after 3 days	458:493	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	8	66	theme	archaea	1707:1713	arg1	smaller					1741:1747	smaller	1741:1747	smaller	1741:1747	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	8	66	theme	archaea	1707:1713	arg1	number					1685:1690	the number	1681:1690	the number of soil fungi, archaea and protists affected	1681:1735	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	2	67	theme	viral	536:540	arg1	groups					542:547	bacterial and viral groups	522:547	bacterial and viral groups	522:547	The relative abundance of bacterial and viral groups was strongly affected by organic material application, whereas that of the archaeal, protist and fungal groups was less affected.					
35788780	9	68	theme	amended	1931:1937	arg1	soil					1939:1942	the maize plant amended soil	1915:1942	the maize plant amended soil	1915:1942	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	6	69	theme	maize	1319:1323	arg1	soil					1339:1342	the young maize plant amended soil	1309:1342	the young maize plant amended soil	1309:1342	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	6	70	from	soil	1339:1342	arg1	enriched					1297:1304	enriched	1297:1304	enriched	1297:1304	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	8	71	theme	protists	1719:1726	arg1	smaller					1741:1747	smaller	1741:1747	smaller	1741:1747	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	8	71	theme	protists	1719:1726	arg1	number					1685:1690	the number	1681:1690	the number of soil fungi, archaea and protists affected	1681:1735	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	2	72	theme	bacterial	522:530	arg1	groups					542:547	bacterial and viral groups	522:547	bacterial and viral groups	522:547	The relative abundance of bacterial and viral groups was strongly affected by organic material application, whereas that of the archaeal, protist and fungal groups was less affected.					
35788780	0	73	theme	metagenomics	140:151	arg1	study					153:157	a metagenomics study	138:157	a metagenomics study	138:157	Application of young maize plant residues alters the microbiome composition and its functioning in a soil under conservation agriculture: a metagenomics study.					
35788780	3	74	with	groups	801:806	arg1	metabolism					865:874	slow growing oligotrophic and chemolithoautotrophic metabolism	813:874	slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea	813:902	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria) were enriched in the amended soil, whereas the groups with slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea were greater in the unamended than in the amended soil.					
35788780	0	75	theme	conservation	112:123	arg1	agriculture					125:135	conservation agriculture	112:135	conservation agriculture	112:135	Application of young maize plant residues alters the microbiome composition and its functioning in a soil under conservation agriculture: a metagenomics study.					
35788780	5	76	theme	dominant	1039:1046	arg1	family					1054:1059	the dominant viral family	1035:1059	the dominant viral family	1035:1059	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	5	76	theme	dominant	1039:1046	arg1	Caudovirales					1018:1029	Caudovirales	1018:1029	Caudovirales	1018:1029	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	3	77	theme	chemolithoautotrophic	843:863	arg1	metabolism					865:874	slow growing oligotrophic and chemolithoautotrophic metabolism	813:874	slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea	813:902	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria) were enriched in the amended soil, whereas the groups with slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea were greater in the unamended than in the amended soil.					
35788780	3	78	theme	oligotrophic	826:837	arg1	metabolism					865:874	slow growing oligotrophic and chemolithoautotrophic metabolism	813:874	slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea	813:902	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria) were enriched in the amended soil, whereas the groups with slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea were greater in the unamended than in the amended soil.					
35788780	6	79	theme	specialized	1174:1184	arg1	traits					1196:1201	More specialized metabolic traits	1169:1201	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes	1169:1290	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	1	80	theme	microbial	335:343	arg1	communities					345:355	the microbial communities	331:355	the microbial communities	331:355	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	4	81	theme	viral	969:973	arg1	structure					975:983	The soil viral structure	960:983	The soil viral structure	960:983	The soil viral structure and richness were also affected.					
35788780	5	82	from	members	1067:1073	arg1	soil					1163:1166	the unamended soil	1149:1166	the unamended soil	1149:1166	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	9	83	theme	nitrification	1997:2009	arg1	process					2011:2017	the nitrification process	1993:2017	the nitrification process	1993:2017	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	1	84	theme	potential	367:375	arg1	functionality					377:389	their potential functionality	361:389	their potential functionality	361:389	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	3	85	dep	enriched	759:766	arg1	whereas					789:795	whereas	789:795	whereas	789:795	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria) were enriched in the amended soil, whereas the groups with slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea were greater in the unamended than in the amended soil.					
35788780	1	86	theme	arable	397:402	arg1	soil					404:407	an arable soil	394:407	an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days	394:493	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	0	87	theme	maize	21:25	arg1	residues					33:40	young maize plant residues	15:40	young maize plant residues	15:40	Application of young maize plant residues alters the microbiome composition and its functioning in a soil under conservation agriculture: a metagenomics study.					
35788780	1	88	theme	shotgun	278:284	arg1	sequencing					298:307	shotgun metagenomic sequencing	278:307	shotgun metagenomic sequencing	278:307	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	7	89	from	groups	1555:1560	arg1	soil					1579:1582	the unamended soil	1565:1582	the unamended soil	1565:1582	Copiotrophic life-style bacterial groups were enriched in the amended soil, whereas oligotrophic life-style bacterial groups in the unamended soil.					
35788780	9	90	theme	organic	1809:1815	arg1	material					1817:1824	organic material	1809:1824	organic material	1809:1824	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	9	91	theme	unamended	2037:2045	arg1	soil					2047:2050	the unamended soil	2033:2050	the unamended soil	2033:2050	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	0	92	theme	residues	33:40	arg1	Application					0:10	Application	0:10	Application of young maize plant residues	0:40	Application of young maize plant residues alters the microbiome composition and its functioning in a soil under conservation agriculture: a metagenomics study.					
35788780	7	93	theme	bacterial	1545:1553	arg1	groups					1555:1560	oligotrophic life-style bacterial groups	1521:1560	oligotrophic life-style bacterial groups in the unamended soil	1521:1582	Copiotrophic life-style bacterial groups were enriched in the amended soil, whereas oligotrophic life-style bacterial groups in the unamended soil.					
35788780	6	94	theme	related	1394:1400	arg1	genes					1402:1406	nitrification related genes	1380:1406	nitrification related genes	1380:1406	More specialized metabolic traits related to both the degradation of complex C compounds and denitrification related genes were enriched in the young maize plant amended soil than in the unamended soil, whereas nitrification related genes were enriched in the latter.					
35788780	5	95	theme	Myoviridae	1135:1144	arg1	members					1067:1073	members	1067:1073	members of Siphoviridae enriched in the amended soil	1067:1118	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	5	95	theme	Myoviridae	1135:1144	arg1	members					1124:1130	members	1124:1130	members of Myoviridae in the unamended soil	1124:1166	Caudovirales was the dominant viral family, with members of Siphoviridae enriched in the amended soil and members of Myoviridae in the unamended soil.					
35788780	7	96	theme	oligotrophic	1521:1532	arg1	groups					1555:1560	oligotrophic life-style bacterial groups	1521:1560	oligotrophic life-style bacterial groups in the unamended soil	1521:1582	Copiotrophic life-style bacterial groups were enriched in the amended soil, whereas oligotrophic life-style bacterial groups in the unamended soil.					
35788780	9	97	theme	relative	1833:1840	arg1	abundance					1842:1850	the relative abundance	1829:1850	the relative abundance of genes involved in the denitrification process	1829:1899	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	9	97	theme	relative	1833:1840	arg1	higher					1905:1910	higher	1905:1910	higher	1905:1910	Metabolic functionality was affected by the application of organic material as the relative abundance of genes involved in the denitrification process was higher in the maize plant amended soil than in the unamended soil and those involved in the nitrification process was higher in the unamended soil.					
35788780	8	98	theme	bacterial	1590:1598	arg1	phylotypes					1610:1619	Many bacterial and viral phylotypes	1585:1619	Many bacterial and viral phylotypes	1585:1619	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	8	99	theme	young	1657:1661	arg1	plants					1669:1674	young maize plants	1657:1674	young maize plants	1657:1674	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	3	100	theme	amended	946:952	arg1	soil					954:957	the amended soil	942:957	the amended soil	942:957	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria) were enriched in the amended soil, whereas the groups with slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea were greater in the unamended than in the amended soil.					
35788780	8	101	theme	viral	1604:1608	arg1	phylotypes					1610:1619	Many bacterial and viral phylotypes	1585:1619	Many bacterial and viral phylotypes	1585:1619	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	8	102	theme	plants	1669:1674	arg1	application					1642:1652	the application	1638:1652	the application of young maize plants	1638:1674	Many bacterial and viral phylotypes were affected by the application of young maize plants, but the number of soil fungi, archaea and protists affected was smaller.					
35788780	1	103	theme	material	216:223	arg1	application					193:203	application	193:203	application of organic material	193:223	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
35788780	7	104	theme	amended	1499:1505	arg1	soil					1507:1510	the amended soil	1495:1510	the amended soil	1495:1510	Copiotrophic life-style bacterial groups were enriched in the amended soil, whereas oligotrophic life-style bacterial groups in the unamended soil.					
35788780	3	105	theme	Cellulose	679:687	arg1	degraders					689:697	Cellulose degraders	679:697	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria)	679:752	Cellulose degraders with copiotrophic lifestyle (e.g., Betaproteobacteria) were enriched in the amended soil, whereas the groups with slow growing oligotrophic and chemolithoautotrophic metabolism within Bacteria and Archaea were greater in the unamended than in the amended soil.					
35788780	1	106	theme	soil	232:235	arg1	populations					247:257	soil microbial populations	232:257	soil microbial populations	232:257	To increase our knowledge on how application of organic material alters soil microbial populations and functionality, shotgun metagenomic sequencing was used to determine the microbial communities and their potential functionality in an arable soil amended with young maize plants (Zea mays L.) in a laboratory experiment after 3 days.					
36968141	7	0	theme	complete	1054:1061	arg1	epitope					1063:1069	the complete epitope	1050:1069	the complete epitope	1050:1069	The structures of full-length mesothelin and its complex with the Fab of MORAb-009 reveal the interaction of the antibody with the complete epitope, which has not been reported previously.					
36968141	4	1	theme	compact	583:589	arg1	solenoid					605:612	a compact, right-handed solenoid	581:612	a compact, right-handed solenoid consisting of 24 short helices and connecting loops	581:664	Here, we report the crystal structures of mesothelin that reveal a compact, right-handed solenoid consisting of 24 short helices and connecting loops.					
36968141	9	2	theme	mesothelin	1329:1338	arg1	fragment					1317:1324	the C-terminal shedding-resistant fragment	1283:1324	the C-terminal shedding-resistant fragment of mesothelin	1283:1338	The structure of the C-terminal shedding-resistant fragment of mesothelin complexed with a mAb 15B6 displays an extended linear epitope and helps explain the protection afforded by the antibody for the shedding sites.					
36968141	6	3	theme	N-glycosylation	835:849	arg1	sites					851:855	all three predicted N-glycosylation sites	815:855	all three predicted N-glycosylation sites	815:855	Glycan attachments have been identified in the structure for all three predicted N-glycosylation sites and confirmed with samples from cell culture and patient ascites.					
36968141	1	4	theme	cell	162:165	arg1	surface					167:173	the cell surface	158:173	the cell surface of many human cancers	158:195	The tumor-associated antigen mesothelin is expressed at high levels on the cell surface of many human cancers, while its expression in normal tissues is limited.					
36968141	5	5	theme	family	737:742	arg1	proteins					744:751	ARM/HEAT family proteins	728:751	ARM/HEAT family proteins	728:751	These helices form a nine-layered spiral coil that resembles ARM/HEAT family proteins.					
36968141	6	6	theme	cell	889:892	arg1	culture					894:900	cell culture	889:900	cell culture	889:900	Glycan attachments have been identified in the structure for all three predicted N-glycosylation sites and confirmed with samples from cell culture and patient ascites.					
36968141	6	7	theme	predicted	825:833	arg1	sites					851:855	all three predicted N-glycosylation sites	815:855	all three predicted N-glycosylation sites	815:855	Glycan attachments have been identified in the structure for all three predicted N-glycosylation sites and confirmed with samples from cell culture and patient ascites.					
36968141	6	8	from	culture	894:900	arg1	samples					876:882	samples	876:882	samples from cell culture and patient ascites	876:920	Glycan attachments have been identified in the structure for all three predicted N-glycosylation sites and confirmed with samples from cell culture and patient ascites.					
36968141	6	9	theme	Glycan	754:759	arg1	attachments					761:771	Glycan attachments	754:771	Glycan attachments	754:771	Glycan attachments have been identified in the structure for all three predicted N-glycosylation sites and confirmed with samples from cell culture and patient ascites.					
36968141	2	10	theme	mesothelin	264:273	arg1	binding					253:259	The binding	249:259	The binding of mesothelin to the tumor-associated cancer antigen 125 (CA-125)	249:325	The binding of mesothelin to the tumor-associated cancer antigen 125 (CA-125) can lead to heterotypic cell adhesion and tumor metastasis within the pleural and peritoneal cavities.					
36968141	11	11	theme	therapeutic	1830:1840	arg1	antibodies					1842:1851	therapeutic antibodies	1830:1851	therapeutic antibodies	1830:1851	It offers insights into the function of mesothelin and guidance for further development of therapeutic antibodies.					
36968141	8	12	theme	specific	1196:1203	arg1	antibodies					1205:1214	specific antibodies	1196:1214	specific antibodies	1196:1214	The N-terminal half of mesothelin is conformationally rigid, suitable for eliciting specific antibodies, whereas its C-terminal portion is more flexible.					
36968141	7	13	with	structures	927:936	arg1	Fab					989:991	the Fab	985:991	the Fab of MORAb-009	985:1004	The structures of full-length mesothelin and its complex with the Fab of MORAb-009 reveal the interaction of the antibody with the complete epitope, which has not been reported previously.					
36968141	10	14	theme	full-length	1515:1525	arg1	mesothelin					1527:1536	full-length mesothelin	1515:1536	full-length mesothelin	1515:1536	Significance The structures of full-length mesothelin and its complexes with antibodies reported here are the first to be determined experimentally, providing atomic models for structural organization of this protein and its interactions with antibodies.					
36968141	9	15	theme	mAb	1357:1359	arg1	15B6					1361:1364	a mAb 15B6	1355:1364	a mAb 15B6	1355:1364	The structure of the C-terminal shedding-resistant fragment of mesothelin complexed with a mAb 15B6 displays an extended linear epitope and helps explain the protection afforded by the antibody for the shedding sites.					
36968141	2	16	theme	tumor	369:373	arg1	metastasis					375:384	tumor metastasis	369:384	tumor metastasis	369:384	The binding of mesothelin to the tumor-associated cancer antigen 125 (CA-125) can lead to heterotypic cell adhesion and tumor metastasis within the pleural and peritoneal cavities.					
36968141	6	17	from	ascites	914:920	arg1	samples					876:882	samples	876:882	samples from cell culture and patient ascites	876:920	Glycan attachments have been identified in the structure for all three predicted N-glycosylation sites and confirmed with samples from cell culture and patient ascites.					
36968141	6	18	theme	patient	906:912	arg1	ascites					914:920	patient ascites	906:920	patient ascites	906:920	Glycan attachments have been identified in the structure for all three predicted N-glycosylation sites and confirmed with samples from cell culture and patient ascites.					
36968141	8	19	dep	rigid	1166:1170	arg1	whereas					1217:1223	whereas	1217:1223	whereas	1217:1223	The N-terminal half of mesothelin is conformationally rigid, suitable for eliciting specific antibodies, whereas its C-terminal portion is more flexible.					
36968141	8	19	dep	rigid	1166:1170	arg1	half					1127:1130	The N-terminal half	1112:1130	The N-terminal half of mesothelin	1112:1144	The N-terminal half of mesothelin is conformationally rigid, suitable for eliciting specific antibodies, whereas its C-terminal portion is more flexible.					
36968141	8	19	dep	rigid	1166:1170	arg1	rigid					1166:1170	rigid	1166:1170	rigid	1166:1170	The N-terminal half of mesothelin is conformationally rigid, suitable for eliciting specific antibodies, whereas its C-terminal portion is more flexible.					
36968141	11	20	theme	guidance	1794:1801	arg1	function					1767:1774	the function	1763:1774	the function of mesothelin and guidance for further development of therapeutic antibodies	1763:1851	It offers insights into the function of mesothelin and guidance for further development of therapeutic antibodies.					
36968141	10	21	theme	protein	1693:1699	arg1	organization					1672:1683	structural organization	1661:1683	structural organization of this protein	1661:1699	Significance The structures of full-length mesothelin and its complexes with antibodies reported here are the first to be determined experimentally, providing atomic models for structural organization of this protein and its interactions with antibodies.					
36968141	10	21	theme	protein	1693:1699	arg1	interactions					1709:1720	its interactions	1705:1720	its interactions with antibodies	1705:1736	Significance The structures of full-length mesothelin and its complexes with antibodies reported here are the first to be determined experimentally, providing atomic models for structural organization of this protein and its interactions with antibodies.					
36968141	9	22	theme	linear	1387:1392	arg1	epitope					1394:1400	an extended linear epitope	1375:1400	an extended linear epitope	1375:1400	The structure of the C-terminal shedding-resistant fragment of mesothelin complexed with a mAb 15B6 displays an extended linear epitope and helps explain the protection afforded by the antibody for the shedding sites.					
36968141	10	23	theme	atomic	1643:1648	arg1	models					1650:1655	atomic models	1643:1655	atomic models for structural organization of this protein and its interactions with antibodies	1643:1736	Significance The structures of full-length mesothelin and its complexes with antibodies reported here are the first to be determined experimentally, providing atomic models for structural organization of this protein and its interactions with antibodies.					
36968141	1	24	theme	many	178:181	arg1	cancers					189:195	many human cancers	178:195	many human cancers	178:195	The tumor-associated antigen mesothelin is expressed at high levels on the cell surface of many human cancers, while its expression in normal tissues is limited.					
36968141	10	25	with	interactions	1709:1720	arg1	antibodies					1727:1736	antibodies	1727:1736	antibodies	1727:1736	Significance The structures of full-length mesothelin and its complexes with antibodies reported here are the first to be determined experimentally, providing atomic models for structural organization of this protein and its interactions with antibodies.					
36968141	4	26	theme	mesothelin	558:567	arg1	structures					544:553	the crystal structures	532:553	the crystal structures of mesothelin that reveal a compact, right-handed solenoid consisting of 24 short helices and connecting loops	532:664	Here, we report the crystal structures of mesothelin that reveal a compact, right-handed solenoid consisting of 24 short helices and connecting loops.					
36968141	7	27	theme	mesothelin	953:962	arg1	structures					927:936	The structures	923:936	The structures of full-length mesothelin	923:962	The structures of full-length mesothelin and its complex with the Fab of MORAb-009 reveal the interaction of the antibody with the complete epitope, which has not been reported previously.					
36968141	7	27	theme	mesothelin	953:962	arg1	complex					972:978	its complex	968:978	its complex with the Fab of MORAb-009	968:1004	The structures of full-length mesothelin and its complex with the Fab of MORAb-009 reveal the interaction of the antibody with the complete epitope, which has not been reported previously.					
36968141	2	28	theme	cancer	299:304	arg1	CA-125					319:324	CA-125	319:324	CA-125	319:324	The binding of mesothelin to the tumor-associated cancer antigen 125 (CA-125) can lead to heterotypic cell adhesion and tumor metastasis within the pleural and peritoneal cavities.					
36968141	2	28	theme	cancer	299:304	arg1	antigen					306:312	the tumor-associated cancer antigen 125	278:316	the tumor-associated cancer antigen 125 (CA-125)	278:325	The binding of mesothelin to the tumor-associated cancer antigen 125 (CA-125) can lead to heterotypic cell adhesion and tumor metastasis within the pleural and peritoneal cavities.					
36968141	2	29	theme	cell	351:354	arg1	adhesion					356:363	heterotypic cell adhesion	339:363	heterotypic cell adhesion	339:363	The binding of mesothelin to the tumor-associated cancer antigen 125 (CA-125) can lead to heterotypic cell adhesion and tumor metastasis within the pleural and peritoneal cavities.					
36968141	0	30	theme	Antigen	21:27	arg1	Mesothelin					29:38	Cancer Antigen Mesothelin	14:38	Cancer Antigen Mesothelin	14:38	Structures of Cancer Antigen Mesothelin and Its Complexes with Therapeutic Antibodies.					
36968141	11	31	theme	antibodies	1842:1851	arg1	development					1815:1825	further development	1807:1825	further development of therapeutic antibodies	1807:1851	It offers insights into the function of mesothelin and guidance for further development of therapeutic antibodies.					
36968141	9	32	theme	shedding	1468:1475	arg1	sites					1477:1481	the shedding sites	1464:1481	the shedding sites	1464:1481	The structure of the C-terminal shedding-resistant fragment of mesothelin complexed with a mAb 15B6 displays an extended linear epitope and helps explain the protection afforded by the antibody for the shedding sites.					
36968141	1	33	theme	antigen	108:114	arg1	mesothelin					116:125	The tumor-associated antigen mesothelin	87:125	The tumor-associated antigen mesothelin	87:125	The tumor-associated antigen mesothelin is expressed at high levels on the cell surface of many human cancers, while its expression in normal tissues is limited.					
36968141	2	34	theme	pleural	397:403	arg1	cavities					420:427	the pleural and peritoneal cavities	393:427	cavities	420:427	The binding of mesothelin to the tumor-associated cancer antigen 125 (CA-125) can lead to heterotypic cell adhesion and tumor metastasis within the pleural and peritoneal cavities.					
36968141	1	35	theme	tumor-associated	91:106	arg1	antigen					108:114	The tumor-associated antigen	87:114	The tumor-associated antigen mesothelin	87:125	The tumor-associated antigen mesothelin is expressed at high levels on the cell surface of many human cancers, while its expression in normal tissues is limited.					
36968141	9	36	theme	extended	1378:1385	arg1	epitope					1394:1400	an extended linear epitope	1375:1400	an extended linear epitope	1375:1400	The structure of the C-terminal shedding-resistant fragment of mesothelin complexed with a mAb 15B6 displays an extended linear epitope and helps explain the protection afforded by the antibody for the shedding sites.					
36968141	10	37	with	Significance	1484:1495	arg1	antibodies					1561:1570	antibodies	1561:1570	antibodies reported here	1561:1584	Significance The structures of full-length mesothelin and its complexes with antibodies reported here are the first to be determined experimentally, providing atomic models for structural organization of this protein and its interactions with antibodies.					
36968141	4	38	theme	short	631:635	arg1	helices					637:643	24 short helices	628:643	24 short helices	628:643	Here, we report the crystal structures of mesothelin that reveal a compact, right-handed solenoid consisting of 24 short helices and connecting loops.					
36968141	7	39	with	complex	972:978	arg1	Fab					989:991	the Fab	985:991	the Fab of MORAb-009	985:1004	The structures of full-length mesothelin and its complex with the Fab of MORAb-009 reveal the interaction of the antibody with the complete epitope, which has not been reported previously.					
36968141	4	40	theme	connecting	649:658	arg1	loops					660:664	connecting loops	649:664	connecting loops	649:664	Here, we report the crystal structures of mesothelin that reveal a compact, right-handed solenoid consisting of 24 short helices and connecting loops.					
36968141	1	41	theme	human	183:187	arg1	cancers					189:195	many human cancers	178:195	many human cancers	178:195	The tumor-associated antigen mesothelin is expressed at high levels on the cell surface of many human cancers, while its expression in normal tissues is limited.					
36968141	3	42	theme	Immunotherapeutic	430:446	arg1	strategies					448:457	Immunotherapeutic strategies	430:457	Immunotherapeutic strategies targeting mesothelin	430:478	Immunotherapeutic strategies targeting mesothelin are being intensively investigated.					
36968141	0	43	theme	Mesothelin	29:38	arg1	Structures					0:9	Structures	0:9	Structures of Cancer Antigen Mesothelin	0:38	Structures of Cancer Antigen Mesothelin and Its Complexes with Therapeutic Antibodies.					
36968141	0	43	theme	Mesothelin	29:38	arg1	Complexes					48:56	Its Complexes	44:56	Its Complexes with Therapeutic Antibodies	44:84	Structures of Cancer Antigen Mesothelin and Its Complexes with Therapeutic Antibodies.					
36968141	5	44	theme	ARM/HEAT	728:735	arg1	proteins					744:751	ARM/HEAT family proteins	728:751	ARM/HEAT family proteins	728:751	These helices form a nine-layered spiral coil that resembles ARM/HEAT family proteins.					
36968141	4	45	dep	compact	583:589	arg1	right-handed					592:603	right-handed	592:603	right-handed	592:603	Here, we report the crystal structures of mesothelin that reveal a compact, right-handed solenoid consisting of 24 short helices and connecting loops.					
36968141	2	46	theme	peritoneal	409:418	arg1	cavities					420:427	the pleural and peritoneal cavities	393:427	cavities	420:427	The binding of mesothelin to the tumor-associated cancer antigen 125 (CA-125) can lead to heterotypic cell adhesion and tumor metastasis within the pleural and peritoneal cavities.					
36968141	7	47	theme	antibody	1036:1043	arg1	interaction					1017:1027	the interaction	1013:1027	the interaction of the antibody with the complete epitope, which has not been reported previously	1013:1109	The structures of full-length mesothelin and its complex with the Fab of MORAb-009 reveal the interaction of the antibody with the complete epitope, which has not been reported previously.					
36968141	10	48	dep	Significance	1484:1495	arg1	structures					1501:1510	The structures	1497:1510	Significance The structures of full-length mesothelin	1484:1536	Significance The structures of full-length mesothelin and its complexes with antibodies reported here are the first to be determined experimentally, providing atomic models for structural organization of this protein and its interactions with antibodies.					
36968141	6	49	gly	N-glycosylation	835:849	arg2	sites					851:855	all three predicted N-glycosylation sites	815:855	all three predicted N-glycosylation sites	815:855	Glycan attachments have been identified in the structure for all three predicted N-glycosylation sites and confirmed with samples from cell culture and patient ascites.					
36968141	6	49	gly	N-glycosylation	835:849	arg2	three					819:823	three	819:823	three	819:823	Glycan attachments have been identified in the structure for all three predicted N-glycosylation sites and confirmed with samples from cell culture and patient ascites.					
36968141	8	50	theme	C-terminal	1229:1238	arg1	portion					1240:1246	its C-terminal portion	1225:1246	its C-terminal portion	1225:1246	The N-terminal half of mesothelin is conformationally rigid, suitable for eliciting specific antibodies, whereas its C-terminal portion is more flexible.					
36968141	5	51	theme	nine-layered	688:699	arg1	coil					708:711	a nine-layered spiral coil	686:711	a nine-layered spiral coil that resembles ARM/HEAT family proteins	686:751	These helices form a nine-layered spiral coil that resembles ARM/HEAT family proteins.					
36968141	10	52	theme	mesothelin	1527:1536	arg1	structures					1501:1510	The structures	1497:1510	Significance The structures of full-length mesothelin	1484:1536	Significance The structures of full-length mesothelin and its complexes with antibodies reported here are the first to be determined experimentally, providing atomic models for structural organization of this protein and its interactions with antibodies.					
36968141	8	53	theme	N-terminal	1116:1125	arg1	whereas					1217:1223	whereas	1217:1223	whereas	1217:1223	The N-terminal half of mesothelin is conformationally rigid, suitable for eliciting specific antibodies, whereas its C-terminal portion is more flexible.					
36968141	8	53	theme	N-terminal	1116:1125	arg1	half					1127:1130	The N-terminal half	1112:1130	The N-terminal half of mesothelin	1112:1144	The N-terminal half of mesothelin is conformationally rigid, suitable for eliciting specific antibodies, whereas its C-terminal portion is more flexible.					
36968141	8	53	theme	N-terminal	1116:1125	arg1	rigid					1166:1170	rigid	1166:1170	rigid	1166:1170	The N-terminal half of mesothelin is conformationally rigid, suitable for eliciting specific antibodies, whereas its C-terminal portion is more flexible.					
36968141	11	54	theme	mesothelin	1779:1788	arg1	function					1767:1774	the function	1763:1774	the function of mesothelin and guidance for further development of therapeutic antibodies	1763:1851	It offers insights into the function of mesothelin and guidance for further development of therapeutic antibodies.					
36968141	4	55	theme	crystal	536:542	arg1	structures					544:553	the crystal structures	532:553	the crystal structures of mesothelin that reveal a compact, right-handed solenoid consisting of 24 short helices and connecting loops	532:664	Here, we report the crystal structures of mesothelin that reveal a compact, right-handed solenoid consisting of 24 short helices and connecting loops.					
36968141	1	56	from	expression	208:217	arg1	tissues					229:235	normal tissues	222:235	normal tissues	222:235	The tumor-associated antigen mesothelin is expressed at high levels on the cell surface of many human cancers, while its expression in normal tissues is limited.					
36968141	8	57	theme	mesothelin	1135:1144	arg1	whereas					1217:1223	whereas	1217:1223	whereas	1217:1223	The N-terminal half of mesothelin is conformationally rigid, suitable for eliciting specific antibodies, whereas its C-terminal portion is more flexible.					
36968141	8	57	theme	mesothelin	1135:1144	arg1	half					1127:1130	The N-terminal half	1112:1130	The N-terminal half of mesothelin	1112:1144	The N-terminal half of mesothelin is conformationally rigid, suitable for eliciting specific antibodies, whereas its C-terminal portion is more flexible.					
36968141	8	57	theme	mesothelin	1135:1144	arg1	rigid					1166:1170	rigid	1166:1170	rigid	1166:1170	The N-terminal half of mesothelin is conformationally rigid, suitable for eliciting specific antibodies, whereas its C-terminal portion is more flexible.					
36968141	0	58	with	Structures	0:9	arg1	Antibodies					75:84	Therapeutic Antibodies	63:84	Therapeutic Antibodies	63:84	Structures of Cancer Antigen Mesothelin and Its Complexes with Therapeutic Antibodies.					
36968141	2	59	theme	tumor-associated	282:297	arg1	CA-125					319:324	CA-125	319:324	CA-125	319:324	The binding of mesothelin to the tumor-associated cancer antigen 125 (CA-125) can lead to heterotypic cell adhesion and tumor metastasis within the pleural and peritoneal cavities.					
36968141	2	59	theme	tumor-associated	282:297	arg1	antigen					306:312	the tumor-associated cancer antigen 125	278:316	the tumor-associated cancer antigen 125 (CA-125)	278:325	The binding of mesothelin to the tumor-associated cancer antigen 125 (CA-125) can lead to heterotypic cell adhesion and tumor metastasis within the pleural and peritoneal cavities.					
36968141	11	60	theme	further	1807:1813	arg1	development					1815:1825	further development	1807:1825	further development of therapeutic antibodies	1807:1851	It offers insights into the function of mesothelin and guidance for further development of therapeutic antibodies.					
36968141	5	61	theme	spiral	701:706	arg1	coil					708:711	a nine-layered spiral coil	686:711	a nine-layered spiral coil that resembles ARM/HEAT family proteins	686:751	These helices form a nine-layered spiral coil that resembles ARM/HEAT family proteins.					
36968141	9	62	theme	C-terminal	1287:1296	arg1	fragment					1317:1324	the C-terminal shedding-resistant fragment	1283:1324	the C-terminal shedding-resistant fragment of mesothelin	1283:1338	The structure of the C-terminal shedding-resistant fragment of mesothelin complexed with a mAb 15B6 displays an extended linear epitope and helps explain the protection afforded by the antibody for the shedding sites.					
36968141	0	63	with	Complexes	48:56	arg1	Antibodies					75:84	Therapeutic Antibodies	63:84	Therapeutic Antibodies	63:84	Structures of Cancer Antigen Mesothelin and Its Complexes with Therapeutic Antibodies.					
36968141	1	64	theme	high	143:146	arg1	levels					148:153	high levels	143:153	high levels	143:153	The tumor-associated antigen mesothelin is expressed at high levels on the cell surface of many human cancers, while its expression in normal tissues is limited.					
36968141	10	65	with	complexes	1546:1554	arg1	antibodies					1561:1570	antibodies	1561:1570	antibodies reported here	1561:1584	Significance The structures of full-length mesothelin and its complexes with antibodies reported here are the first to be determined experimentally, providing atomic models for structural organization of this protein and its interactions with antibodies.					
36968141	10	66	with	organization	1672:1683	arg1	antibodies					1727:1736	antibodies	1727:1736	antibodies	1727:1736	Significance The structures of full-length mesothelin and its complexes with antibodies reported here are the first to be determined experimentally, providing atomic models for structural organization of this protein and its interactions with antibodies.					
36968141	0	67	theme	Therapeutic	63:73	arg1	Antibodies					75:84	Therapeutic Antibodies	63:84	Therapeutic Antibodies	63:84	Structures of Cancer Antigen Mesothelin and Its Complexes with Therapeutic Antibodies.					
36968141	9	68	theme	shedding-resistant	1298:1315	arg1	fragment					1317:1324	the C-terminal shedding-resistant fragment	1283:1324	the C-terminal shedding-resistant fragment of mesothelin	1283:1338	The structure of the C-terminal shedding-resistant fragment of mesothelin complexed with a mAb 15B6 displays an extended linear epitope and helps explain the protection afforded by the antibody for the shedding sites.					
36968141	10	69	theme	structural	1661:1670	arg1	organization					1672:1683	structural organization	1661:1683	structural organization of this protein	1661:1699	Significance The structures of full-length mesothelin and its complexes with antibodies reported here are the first to be determined experimentally, providing atomic models for structural organization of this protein and its interactions with antibodies.					
36968141	1	70	theme	cancers	189:195	arg1	surface					167:173	the cell surface	158:173	the cell surface of many human cancers	158:195	The tumor-associated antigen mesothelin is expressed at high levels on the cell surface of many human cancers, while its expression in normal tissues is limited.					
36968141	2	71	theme	heterotypic	339:349	arg1	adhesion					356:363	heterotypic cell adhesion	339:363	heterotypic cell adhesion	339:363	The binding of mesothelin to the tumor-associated cancer antigen 125 (CA-125) can lead to heterotypic cell adhesion and tumor metastasis within the pleural and peritoneal cavities.					
36968141	7	72	theme	full-length	941:951	arg1	mesothelin					953:962	full-length mesothelin	941:962	full-length mesothelin	941:962	The structures of full-length mesothelin and its complex with the Fab of MORAb-009 reveal the interaction of the antibody with the complete epitope, which has not been reported previously.					
36968141	1	73	theme	normal	222:227	arg1	tissues					229:235	normal tissues	222:235	normal tissues	222:235	The tumor-associated antigen mesothelin is expressed at high levels on the cell surface of many human cancers, while its expression in normal tissues is limited.					
36968141	9	74	theme	fragment	1317:1324	arg1	structure					1270:1278	The structure	1266:1278	The structure of the C-terminal shedding-resistant fragment of mesothelin complexed with a mAb 15B6	1266:1364	The structure of the C-terminal shedding-resistant fragment of mesothelin complexed with a mAb 15B6 displays an extended linear epitope and helps explain the protection afforded by the antibody for the shedding sites.					
36968141	0	75	theme	Cancer	14:19	arg1	Mesothelin					29:38	Cancer Antigen Mesothelin	14:38	Cancer Antigen Mesothelin	14:38	Structures of Cancer Antigen Mesothelin and Its Complexes with Therapeutic Antibodies.					
36968141	7	76	theme	MORAb-009	996:1004	arg1	Fab					989:991	the Fab	985:991	the Fab of MORAb-009	985:1004	The structures of full-length mesothelin and its complex with the Fab of MORAb-009 reveal the interaction of the antibody with the complete epitope, which has not been reported previously.					
36968141	7	77	with	interaction	1017:1027	arg1	epitope					1063:1069	the complete epitope	1050:1069	the complete epitope	1050:1069	The structures of full-length mesothelin and its complex with the Fab of MORAb-009 reveal the interaction of the antibody with the complete epitope, which has not been reported previously.					
35927436	6	0	link	O-linked	747:754	arg1	glycans					756:762	Multiple O-linked glycans	738:762	Multiple O-linked glycans	738:762	Multiple O-linked glycans are observed within N-linked glycosylation sequons, suggesting crosstalk between the two types of modifications.					
35927436	6	1	theme	O-linked	747:754	arg1	glycans					756:762	Multiple O-linked glycans	738:762	Multiple O-linked glycans	738:762	Multiple O-linked glycans are observed within N-linked glycosylation sequons, suggesting crosstalk between the two types of modifications.					
35927436	3	2	theme	serum	494:498	arg1	antibodies					500:509	serum antibodies	494:509	serum antibodies	494:509	Glycosylation is important for host cell attachment, GP stability and fusion activity, and shielding from neutralization by serum antibodies.					
35927436	2	3	dep	17	232:233	arg1	to					229:230	to	229:230	to	229:230	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	4	4	theme	GP	607:608	arg1	patterns					584:591	the site-specific glycosylation patterns	552:591	the site-specific glycosylation patterns of ebolavirus GP	552:608	Here, we use glycoproteomics to profile the site-specific glycosylation patterns of ebolavirus GP.					
35927436	2	5	theme	O-linked	260:267	arg1	glycans					269:275	numerous O-linked glycans	251:275	numerous O-linked glycans in its disordered mucin-like domain (MLD)	251:317	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	2	5	theme	O-linked	260:267	arg1	sites					244:248	up to 17 N-linked sites	226:248	up to 17 N-linked sites	226:248	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	1	6	theme	antibody	175:182	arg1	response					184:191	the host antibody response	166:191	the host antibody response	166:191	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	2	7	gly	glycosylated	208:219	arg1	GP					194:195	GP	194:195	GP	194:195	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	9	8	theme	C-linked	1197:1204	arg1	glycans					1206:1212	C-linked glycans	1197:1212	C-linked glycans	1197:1212	Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.					
35927436	5	9	theme	O-linked	637:644	arg1	sites					660:664	up to 16 unique O-linked glycosylation sites	621:664	up to 16 unique O-linked glycosylation sites in the MLD	621:675	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	1	10	theme	host	124:127	arg1	entry					134:138	host cell entry	124:138	host cell entry	124:138	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	9	11	link	C-linked	1197:1204	arg1	glycans					1206:1212	C-linked glycans	1197:1212	C-linked glycans	1197:1212	Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.					
35927436	5	12	theme	GP1	725:727	arg1	subunit					729:735	the receptor-binding GP1 subunit	704:735	the receptor-binding GP1 subunit	704:735	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	8	13	theme	sites	1000:1004	arg1	majority					979:986	the majority	975:986	the majority of N-linked sites	975:1004	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	8	14	from	glycans	1086:1092	arg1	sites					1027:1031	the conserved sites	1013:1031	the conserved sites N257 and especially N563	1013:1056	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	8	14	from	glycans	1086:1092	arg1	enriched					1062:1069	enriched	1062:1069	enriched	1062:1069	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	6	15	located	observed	768:775	arg1	sequons					807:813	N-linked glycosylation sequons	784:813	N-linked glycosylation sequons	784:813	Multiple O-linked glycans are observed within N-linked glycosylation sequons, suggesting crosstalk between the two types of modifications.					
35927436	6	15	located	observed	768:775	arg2	glycans					756:762	Multiple O-linked glycans	738:762	Multiple O-linked glycans	738:762	Multiple O-linked glycans are observed within N-linked glycosylation sequons, suggesting crosstalk between the two types of modifications.					
35927436	7	16	theme	trimeric	929:936	arg1	GP					938:939	full-length trimeric GP	917:939	full-length trimeric GP	917:939	We confirmed C-mannosylation of W288 in full-length trimeric GP.					
35927436	2	17	theme	mannosylation	349:361	arg1	sites					363:367	three predicted C-linked mannosylation sites	324:367	three predicted C-linked mannosylation sites	324:367	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	9	18	theme	future	1276:1281	arg1	studies					1297:1303	future immunological studies	1276:1303	future immunological studies	1276:1303	Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.					
35927436	0	19	theme	envelope	32:39	arg1	GP					54:55	the ebolavirus envelope glycoprotein GP	17:55	the ebolavirus envelope glycoprotein GP	17:55	Glycan shield of the ebolavirus envelope glycoprotein GP.					
35927436	1	20	theme	glycoprotein	71:82	arg1	essential					110:118	essential	110:118	essential	110:118	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	1	20	theme	glycoprotein	71:82	arg1	GP					84:85	The envelope glycoprotein GP	58:85	The envelope glycoprotein GP of the ebolaviruses	58:105	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	6	21	gly	glycosylation	793:805	arg2	sequons					807:813	N-linked glycosylation sequons	784:813	N-linked glycosylation sequons	784:813	Multiple O-linked glycans are observed within N-linked glycosylation sequons, suggesting crosstalk between the two types of modifications.					
35927436	2	22	theme	predicted	330:338	arg1	sites					363:367	three predicted C-linked mannosylation sites	324:367	three predicted C-linked mannosylation sites	324:367	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	3	23	theme	GP	423:424	arg1	stability					426:434	GP stability	423:434	GP stability	423:434	Glycosylation is important for host cell attachment, GP stability and fusion activity, and shielding from neutralization by serum antibodies.					
35927436	9	24	theme	functional	1309:1318	arg1	interpretation					1320:1333	functional interpretation	1309:1333	functional interpretation of ebolavirus GP-antibody interactions	1309:1372	Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.					
35927436	1	25	theme	ebolaviruses	94:105	arg1	essential					110:118	essential	110:118	essential	110:118	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	1	25	theme	ebolaviruses	94:105	arg1	GP					84:85	The envelope glycoprotein GP	58:85	The envelope glycoprotein GP of the ebolaviruses	58:105	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	0	26	theme	ebolavirus	21:30	arg1	GP					54:55	the ebolavirus envelope glycoprotein GP	17:55	the ebolavirus envelope glycoprotein GP	17:55	Glycan shield of the ebolavirus envelope glycoprotein GP.					
35927436	2	27	link	C-linked	340:347	arg1	sites					363:367	three predicted C-linked mannosylation sites	324:367	three predicted C-linked mannosylation sites	324:367	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	0	28	theme	glycoprotein	41:52	arg1	GP					54:55	the ebolavirus envelope glycoprotein GP	17:55	the ebolavirus envelope glycoprotein GP	17:55	Glycan shield of the ebolavirus envelope glycoprotein GP.					
35927436	9	29	theme	GP-antibody	1349:1359	arg1	interactions					1361:1372	ebolavirus GP-antibody interactions	1338:1372	ebolavirus GP-antibody interactions	1338:1372	Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.					
35927436	2	30	theme	mucin-like	295:304	arg1	domain					306:311	its disordered mucin-like domain	280:311	its disordered mucin-like domain (MLD)	280:317	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	2	30	theme	mucin-like	295:304	arg1	MLD					314:316	MLD	314:316	MLD	314:316	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	2	31	theme	N-linked	235:242	arg1	glycans					269:275	numerous O-linked glycans	251:275	numerous O-linked glycans in its disordered mucin-like domain (MLD)	251:317	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	2	31	theme	N-linked	235:242	arg1	sites					244:248	up to 17 N-linked sites	226:248	up to 17 N-linked sites	226:248	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	6	32	theme	glycosylation	793:805	arg1	sequons					807:813	N-linked glycosylation sequons	784:813	N-linked glycosylation sequons	784:813	Multiple O-linked glycans are observed within N-linked glycosylation sequons, suggesting crosstalk between the two types of modifications.					
35927436	4	33	theme	site-specific	556:568	arg1	patterns					584:591	the site-specific glycosylation patterns	552:591	the site-specific glycosylation patterns of ebolavirus GP	552:608	Here, we use glycoproteomics to profile the site-specific glycosylation patterns of ebolavirus GP.					
35927436	2	34	from	glycans	269:275	arg1	domain					306:311	its disordered mucin-like domain	280:311	its disordered mucin-like domain (MLD)	280:317	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	2	34	from	glycans	269:275	arg1	MLD					314:316	MLD	314:316	MLD	314:316	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	8	35	dep	sites	1027:1031	arg1	sites					1027:1031	the conserved sites	1013:1031	the conserved sites N257 and especially N563	1013:1056	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	8	35	dep	sites	1027:1031	arg1	N257					1033:1036	N257	1033:1036	N257	1033:1036	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	8	35	dep	sites	1027:1031	arg1	N563					1053:1056	N563	1053:1056	N563	1053:1056	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	5	36	theme	receptor-binding	708:723	arg1	subunit					729:735	the receptor-binding GP1 subunit	704:735	the receptor-binding GP1 subunit	704:735	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	8	37	theme	complex	950:956	arg1	glycosylation					958:970	complex glycosylation	950:970	complex glycosylation	950:970	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	5	38	link	O-linked	686:693	arg1	sites					695:699	two O-linked sites	682:699	two O-linked sites in the receptor-binding GP1 subunit	682:735	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	8	39	theme	host-cell	1116:1124	arg1	attachment					1126:1135	host-cell attachment	1116:1135	host-cell attachment via DC-SIGN/L-SIGN	1116:1154	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	6	40	theme	modifications	862:874	arg1	types					853:857	the two types	845:857	the two types of modifications	845:874	Multiple O-linked glycans are observed within N-linked glycosylation sequons, suggesting crosstalk between the two types of modifications.					
35927436	3	41	from	neutralization	476:489	arg1	activity					447:454	fusion activity	440:454	fusion activity	440:454	Glycosylation is important for host cell attachment, GP stability and fusion activity, and shielding from neutralization by serum antibodies.					
35927436	3	41	from	neutralization	476:489	arg1	shielding					461:469	shielding	461:469	shielding from neutralization by serum antibodies	461:509	Glycosylation is important for host cell attachment, GP stability and fusion activity, and shielding from neutralization by serum antibodies.					
35927436	3	41	from	neutralization	476:489	arg1	attachment					411:420	host cell attachment	401:420	host cell attachment	401:420	Glycosylation is important for host cell attachment, GP stability and fusion activity, and shielding from neutralization by serum antibodies.					
35927436	3	41	from	neutralization	476:489	arg1	stability					426:434	GP stability	423:434	GP stability	423:434	Glycosylation is important for host cell attachment, GP stability and fusion activity, and shielding from neutralization by serum antibodies.					
35927436	1	42	theme	primary	148:154	arg1	target					156:161	the primary target	144:161	the primary target of the host antibody response	144:191	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	6	43	theme	Multiple	738:745	arg1	glycans					756:762	Multiple O-linked glycans	738:762	Multiple O-linked glycans	738:762	Multiple O-linked glycans are observed within N-linked glycosylation sequons, suggesting crosstalk between the two types of modifications.					
35927436	8	44	theme	N-linked	991:998	arg1	sites					1000:1004	N-linked sites	991:1004	N-linked sites	991:1004	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	8	45	gly	glycosylation	958:970	arg1	majority					979:986	the majority	975:986	the majority of N-linked sites	975:1004	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	2	46	link	N-linked	235:242	arg1	glycans					269:275	numerous O-linked glycans	251:275	numerous O-linked glycans in its disordered mucin-like domain (MLD)	251:317	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	2	46	link	N-linked	235:242	arg1	sites					244:248	up to 17 N-linked sites	226:248	up to 17 N-linked sites	226:248	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	1	47	theme	host	170:173	arg1	response					184:191	the host antibody response	166:191	the host antibody response	166:191	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	4	48	theme	ebolavirus	596:605	arg1	GP					607:608	ebolavirus GP	596:608	ebolavirus GP	596:608	Here, we use glycoproteomics to profile the site-specific glycosylation patterns of ebolavirus GP.					
35927436	5	49	from	sites	695:699	arg1	subunit					729:735	the receptor-binding GP1 subunit	704:735	the receptor-binding GP1 subunit	704:735	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	5	49	from	sites	695:699	arg1	MLD					673:675	the MLD	669:675	the MLD	669:675	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	1	50	theme	response	184:191	arg1	entry					134:138	host cell entry	124:138	host cell entry	124:138	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	1	50	theme	response	184:191	arg1	target					156:161	the primary target	144:161	the primary target of the host antibody response	144:191	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	2	51	link	O-linked	260:267	arg1	glycans					269:275	numerous O-linked glycans	251:275	numerous O-linked glycans in its disordered mucin-like domain (MLD)	251:317	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	2	51	link	O-linked	260:267	arg1	sites					244:248	up to 17 N-linked sites	226:248	up to 17 N-linked sites	226:248	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	5	52	theme	unique	630:635	arg1	sites					660:664	up to 16 unique O-linked glycosylation sites	621:664	up to 16 unique O-linked glycosylation sites in the MLD	621:675	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	7	53	gly	C-mannosylation	890:904	arg1	GP					938:939	full-length trimeric GP	917:939	full-length trimeric GP	917:939	We confirmed C-mannosylation of W288 in full-length trimeric GP.					
35927436	7	53	gly	C-mannosylation	890:904	arg1	W288					909:912	W288	909:912	W288	909:912	We confirmed C-mannosylation of W288 in full-length trimeric GP.					
35927436	5	54	theme	glycosylation	646:658	arg1	sites					660:664	up to 16 unique O-linked glycosylation sites	621:664	up to 16 unique O-linked glycosylation sites in the MLD	621:675	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	8	55	from	enriched	1062:1069	arg1	glycans					1086:1092	unprocessed glycans	1074:1092	unprocessed glycans	1074:1092	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	9	56	theme	heterogeneous	1233:1245	arg1	glycan					1247:1252	the heterogeneous glycan shield	1229:1259	the heterogeneous glycan shield of GP	1229:1265	Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.					
35927436	1	57	theme	cell	129:132	arg1	entry					134:138	host cell entry	124:138	host cell entry	124:138	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	3	58	theme	fusion	440:445	arg1	activity					447:454	fusion activity	440:454	fusion activity	440:454	Glycosylation is important for host cell attachment, GP stability and fusion activity, and shielding from neutralization by serum antibodies.					
35927436	1	59	gly	glycoprotein	71:82	arg1	glycoprotein					71:82	The envelope glycoprotein GP	58:85	The envelope glycoprotein GP of the ebolaviruses	58:105	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	5	60	gly	glycosylation	646:658	arg2	16					627:628	16	627:628	16	627:628	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	5	60	gly	glycosylation	646:658	arg2	sites					660:664	up to 16 unique O-linked glycosylation sites	621:664	up to 16 unique O-linked glycosylation sites in the MLD	621:675	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	9	61	theme	GP	1264:1265	arg1	glycan					1247:1252	the heterogeneous glycan shield	1229:1259	the heterogeneous glycan shield of GP	1229:1265	Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.					
35927436	4	62	gly	glycosylation	570:582	arg1	GP					607:608	ebolavirus GP	596:608	ebolavirus GP	596:608	Here, we use glycoproteomics to profile the site-specific glycosylation patterns of ebolavirus GP.					
35927436	2	63	theme	disordered	284:293	arg1	domain					306:311	its disordered mucin-like domain	280:311	its disordered mucin-like domain (MLD)	280:317	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	2	63	theme	disordered	284:293	arg1	MLD					314:316	MLD	314:316	MLD	314:316	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	5	64	theme	O-linked	686:693	arg1	sites					695:699	two O-linked sites	682:699	two O-linked sites in the receptor-binding GP1 subunit	682:735	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	1	65	theme	envelope	62:69	arg1	essential					110:118	essential	110:118	essential	110:118	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	1	65	theme	envelope	62:69	arg1	GP					84:85	The envelope glycoprotein GP	58:85	The envelope glycoprotein GP of the ebolaviruses	58:105	The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response.					
35927436	2	66	gly	mannosylation	349:361	arg2	sites					363:367	three predicted C-linked mannosylation sites	324:367	three predicted C-linked mannosylation sites	324:367	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	2	67	theme	C-linked	340:347	arg1	sites					363:367	three predicted C-linked mannosylation sites	324:367	three predicted C-linked mannosylation sites	324:367	GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites.					
35927436	9	68	theme	immunological	1283:1295	arg1	studies					1297:1303	future immunological studies	1276:1303	future immunological studies	1276:1303	Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.					
35927436	7	69	theme	full-length	917:927	arg1	GP					938:939	full-length trimeric GP	917:939	full-length trimeric GP	917:939	We confirmed C-mannosylation of W288 in full-length trimeric GP.					
35927436	5	70	dep	16	627:628	arg1	to					624:625	to	624:625	to	624:625	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	7	71	theme	W288	909:912	arg1	C-mannosylation					890:904	C-mannosylation	890:904	C-mannosylation of W288 in full-length trimeric GP	890:939	We confirmed C-mannosylation of W288 in full-length trimeric GP.					
35927436	9	72	dep	glycan	1247:1252	arg1	shield					1254:1259	shield	1254:1259	shield	1254:1259	Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.					
35927436	7	73	from	C-mannosylation	890:904	arg1	GP					938:939	full-length trimeric GP	917:939	full-length trimeric GP	917:939	We confirmed C-mannosylation of W288 in full-length trimeric GP.					
35927436	8	74	link	N-linked	991:998	arg1	sites					1000:1004	N-linked sites	991:1004	N-linked sites	991:1004	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	8	75	from	role	1108:1111	arg1	attachment					1126:1135	host-cell attachment	1116:1135	host-cell attachment via DC-SIGN/L-SIGN	1116:1154	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	3	76	theme	host	401:404	arg1	attachment					411:420	host cell attachment	401:420	host cell attachment	401:420	Glycosylation is important for host cell attachment, GP stability and fusion activity, and shielding from neutralization by serum antibodies.					
35927436	9	77	theme	ebolavirus	1338:1347	arg1	interactions					1361:1372	ebolavirus GP-antibody interactions	1338:1372	ebolavirus GP-antibody interactions	1338:1372	Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.					
35927436	8	78	theme	unprocessed	1074:1084	arg1	glycans					1086:1092	unprocessed glycans	1074:1092	unprocessed glycans	1074:1092	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	8	79	theme	conserved	1017:1025	arg1	sites					1027:1031	the conserved sites	1013:1031	the conserved sites N257 and especially N563	1013:1056	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	8	79	theme	conserved	1017:1025	arg1	enriched					1062:1069	enriched	1062:1069	enriched	1062:1069	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	8	79	theme	conserved	1017:1025	arg1	N257					1033:1036	N257	1033:1036	N257	1033:1036	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	8	79	theme	conserved	1017:1025	arg1	N563					1053:1056	N563	1053:1056	N563	1053:1056	We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN.					
35927436	9	80	theme	interactions	1361:1372	arg1	studies					1297:1303	future immunological studies	1276:1303	future immunological studies	1276:1303	Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.					
35927436	9	80	theme	interactions	1361:1372	arg1	interpretation					1320:1333	functional interpretation	1309:1333	functional interpretation of ebolavirus GP-antibody interactions	1309:1372	Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.					
35927436	4	81	theme	glycosylation	570:582	arg1	patterns					584:591	the site-specific glycosylation patterns	552:591	the site-specific glycosylation patterns of ebolavirus GP	552:608	Here, we use glycoproteomics to profile the site-specific glycosylation patterns of ebolavirus GP.					
35927436	5	82	link	O-linked	637:644	arg1	sites					660:664	up to 16 unique O-linked glycosylation sites	621:664	up to 16 unique O-linked glycosylation sites in the MLD	621:675	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	5	83	from	sites	660:664	arg1	subunit					729:735	the receptor-binding GP1 subunit	704:735	the receptor-binding GP1 subunit	704:735	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	5	83	from	sites	660:664	arg1	MLD					673:675	the MLD	669:675	the MLD	669:675	We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit.					
35927436	6	84	theme	N-linked	784:791	arg1	sequons					807:813	N-linked glycosylation sequons	784:813	N-linked glycosylation sequons	784:813	Multiple O-linked glycans are observed within N-linked glycosylation sequons, suggesting crosstalk between the two types of modifications.					
35927436	0	85	gly	glycoprotein	41:52	arg1	glycoprotein					41:52	the ebolavirus envelope glycoprotein GP	17:55	the ebolavirus envelope glycoprotein GP	17:55	Glycan shield of the ebolavirus envelope glycoprotein GP.					
35927436	3	86	theme	cell	406:409	arg1	attachment					411:420	host cell attachment	401:420	host cell attachment	401:420	Glycosylation is important for host cell attachment, GP stability and fusion activity, and shielding from neutralization by serum antibodies.					
35927436	6	87	link	N-linked	784:791	arg1	sequons					807:813	N-linked glycosylation sequons	784:813	N-linked glycosylation sequons	784:813	Multiple O-linked glycans are observed within N-linked glycosylation sequons, suggesting crosstalk between the two types of modifications.					
36095034	4	0	from	ratios	760:765	arg1	possible					937:944	possible	937:944	possible	937:944	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	2	1	theme	drugs	408:412	arg1	development					375:385	the development	371:385	the development of anti-inflammatory drugs	371:412	Considering its ethnopharmacological application, it constitutes an important source of biologically active molecules in the development of anti-inflammatory drugs.					
36095034	4	2	theme	organic	1033:1039	arg1	acids					1041:1045	organic acids	1033:1045	organic acids	1033:1045	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	3	3	dep	lineage	628:634	arg1	the					617:619	the	617:619	the	617:619	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	4	4	theme	chromatographic	890:904	arg1	profile					906:912	chromatographic profile	890:912	chromatographic profile	890:912	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	4	5	theme	spectrometry	842:853	arg1	analysis					855:862	Ultrahigh-resolution mass spectrometry analysis	816:862	Ultrahigh-resolution mass spectrometry analysis	816:862	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	3	6	theme	lineage	628:634	arg1	mice					609:612	mice	609:612	mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	609:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	7	with	model	598:602	arg1	mice					609:612	mice	609:612	mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	609:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	4	8	theme	mass	837:840	arg1	analysis					855:862	Ultrahigh-resolution mass spectrometry analysis	816:862	Ultrahigh-resolution mass spectrometry analysis	816:862	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	3	9	theme	anti-inflammatory	543:559	arg1	potential					561:569	the anti-inflammatory potential	539:569	the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	539:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	10	theme	potential	561:569	arg1	methodology					452:462	a dereplication methodology	436:462	a dereplication methodology of the bioactive extract from S. brasiliensis leaves	436:515	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	10	theme	potential	561:569	arg1	evaluation					525:534	the evaluation	521:534	the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	521:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	11	from	model	598:602	arg1	methodology					452:462	a dereplication methodology	436:462	a dereplication methodology of the bioactive extract from S. brasiliensis leaves	436:515	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	11	from	model	598:602	arg1	evaluation					525:534	the evaluation	521:534	the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	521:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	1	12	theme	Siparuna	88:95	arg1	brasiliensis					97:108	Siparuna brasiliensis	88:108	Siparuna brasiliensis	88:108	Siparuna brasiliensis is a medicinal plant widely used by indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies.					
36095034	1	12	theme	Siparuna	88:95	arg1	plant					125:129	a medicinal plant	113:129	a medicinal plant widely used by indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies	113:247	Siparuna brasiliensis is a medicinal plant widely used by indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies.					
36095034	1	13	theme	rainforest	183:192	arg1	communities					157:167	indigenous communities	146:167	indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies	146:247	Siparuna brasiliensis is a medicinal plant widely used by indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies.					
36095034	3	14	theme	dereplication	438:450	arg1	methodology					452:462	a dereplication methodology	436:462	a dereplication methodology of the bioactive extract from S. brasiliensis leaves	436:515	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	15	from	methodology	452:462	arg1	brasiliensis					497:508	S. brasiliensis	494:508	S. brasiliensis leaves	494:515	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	15	from	methodology	452:462	arg1	model					598:602	an in vivo inflammatory model	574:602	an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	574:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	5	16	theme	cell	1212:1215	arg1	viability					1217:1225	cell viability	1212:1225	cell viability	1212:1225	The administration of the extract significantly inhibited carrageenan-induced paw edema and showed effects similar to those of drug dexamethasone without affecting cell viability.					
36095034	3	17	from	potential	561:569	arg1	model					598:602	an in vivo inflammatory model	574:602	an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	574:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	2	18	theme	molecules	358:366	arg1	source					328:333	an important source	315:333	an important source of biologically active molecules	315:366	Considering its ethnopharmacological application, it constitutes an important source of biologically active molecules in the development of anti-inflammatory drugs.					
36095034	1	19	theme	inflammatory	203:214	arg1	diseases					216:223	inflammatory diseases	203:223	inflammatory diseases	203:223	Siparuna brasiliensis is a medicinal plant widely used by indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies.					
36095034	0	20	theme	brasiliensis	26:37	arg1	extract					39:45	Siparuna brasiliensis extract	17:45	Siparuna brasiliensis extract	17:45	Dereplication of Siparuna brasiliensis extract with in vivo anti-inflammatory activity.					
36095034	4	21	from	composition	787:797	arg1	possible					937:944	possible	937:944	possible	937:944	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	3	22	theme	BALB/c	621:626	arg1	lineage					628:634	BALB/c lineage	621:634	BALB/c lineage	621:634	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	5	23	theme	paw	1126:1128	arg1	edema					1130:1134	carrageenan-induced paw edema	1106:1134	carrageenan-induced paw edema	1106:1134	The administration of the extract significantly inhibited carrageenan-induced paw edema and showed effects similar to those of drug dexamethasone without affecting cell viability.					
36095034	0	24	theme	Siparuna	17:24	arg1	extract					39:45	Siparuna brasiliensis extract	17:45	Siparuna brasiliensis extract	17:45	Dereplication of Siparuna brasiliensis extract with in vivo anti-inflammatory activity.					
36095034	2	25	theme	important	318:326	arg1	source					328:333	an important source	315:333	an important source of biologically active molecules	315:366	Considering its ethnopharmacological application, it constitutes an important source of biologically active molecules in the development of anti-inflammatory drugs.					
36095034	1	26	theme	Amazon	176:181	arg1	rainforest					183:192	the Amazon rainforest	172:192	the Amazon rainforest to treat inflammatory diseases and related pathologies	172:247	Siparuna brasiliensis is a medicinal plant widely used by indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies.					
36095034	4	27	with	polyphenols	958:968	arg1	properties					993:1002	anti-inflammatory properties	975:1002	anti-inflammatory properties classified as flavonoids and organic acids	975:1045	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	4	28	theme	charge-to-mass	745:758	arg1	ratios					760:765	their charge-to-mass ratios	739:765	their charge-to-mass ratios (m/z)	739:771	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	4	28	theme	charge-to-mass	745:758	arg1	m/z					768:770	m/z	768:770	m/z	768:770	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	3	29	theme	bioactive	471:479	arg1	extract					481:487	the bioactive extract	467:487	the bioactive extract from S. brasiliensis leaves	467:515	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	5	30	theme	drug	1175:1178	arg1	dexamethasone					1180:1192	drug dexamethasone	1175:1192	drug dexamethasone	1175:1192	The administration of the extract significantly inhibited carrageenan-induced paw edema and showed effects similar to those of drug dexamethasone without affecting cell viability.					
36095034	2	31	theme	ethnopharmacological	266:285	arg1	application					287:297	its ethnopharmacological application	262:297	its ethnopharmacological application	262:297	Considering its ethnopharmacological application, it constitutes an important source of biologically active molecules in the development of anti-inflammatory drugs.					
36095034	1	32	theme	medicinal	115:123	arg1	brasiliensis					97:108	Siparuna brasiliensis	88:108	Siparuna brasiliensis	88:108	Siparuna brasiliensis is a medicinal plant widely used by indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies.					
36095034	1	32	theme	medicinal	115:123	arg1	plant					125:129	a medicinal plant	113:129	a medicinal plant widely used by indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies	113:247	Siparuna brasiliensis is a medicinal plant widely used by indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies.					
36095034	1	33	theme	related	229:235	arg1	pathologies					237:247	related pathologies	229:247	related pathologies	229:247	Siparuna brasiliensis is a medicinal plant widely used by indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies.					
36095034	0	34	theme	extract	39:45	arg1	Dereplication					0:12	Dereplication	0:12	Dereplication of Siparuna brasiliensis extract with in vivo anti-inflammatory activity.	0:86	Dereplication of Siparuna brasiliensis extract with in vivo anti-inflammatory activity.					
36095034	3	35	theme	extract	481:487	arg1	methodology					452:462	a dereplication methodology	436:462	a dereplication methodology of the bioactive extract from S. brasiliensis leaves	436:515	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	35	theme	extract	481:487	arg1	evaluation					525:534	the evaluation	521:534	the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	521:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	4	36	theme	-Orbitrap	873:881	arg1	MS					883:884	ESI(-)-Orbitrap MS	867:884	MS	883:884	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	4	37	theme	Ultrahigh-resolution	816:835	arg1	analysis					855:862	Ultrahigh-resolution mass spectrometry analysis	816:862	Ultrahigh-resolution mass spectrometry analysis	816:862	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	0	38	theme	anti-inflammatory	60:76	arg1	activity					78:85	in vivo anti-inflammatory activity	52:85	in vivo anti-inflammatory activity	52:85	Dereplication of Siparuna brasiliensis extract with in vivo anti-inflammatory activity.					
36095034	3	39	dep	brasiliensis	497:508	arg1	leaves					510:515	leaves	510:515	leaves	510:515	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	2	40	theme	anti-inflammatory	390:406	arg1	drugs					408:412	anti-inflammatory drugs	390:412	anti-inflammatory drugs	390:412	Considering its ethnopharmacological application, it constitutes an important source of biologically active molecules in the development of anti-inflammatory drugs.					
36095034	0	41	theme	in vivo	52:58	arg1	activity					78:85	in vivo anti-inflammatory activity	52:85	in vivo anti-inflammatory activity	52:85	Dereplication of Siparuna brasiliensis extract with in vivo anti-inflammatory activity.					
36095034	3	42	theme	cell	655:658	arg1	lines					660:664	cell lines	655:664	cell lines	655:664	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	43	theme	in vivo	577:583	arg1	model					598:602	an in vivo inflammatory model	574:602	an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	574:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	44	dep	mice	609:612	arg1	using					649:653	using	649:653	using cell lines	649:664	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	44	dep	mice	609:612	arg1	determining					678:688	determining	678:688	determining the production of an inflammatory mediator	678:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	4	45	theme	anti-inflammatory	975:991	arg1	properties					993:1002	anti-inflammatory properties	975:1002	anti-inflammatory properties classified as flavonoids and organic acids	975:1045	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	5	46	theme	similar	1155:1161	arg1	effects					1147:1153	effects	1147:1153	effects similar to those of drug dexamethasone	1147:1192	The administration of the extract significantly inhibited carrageenan-induced paw edema and showed effects similar to those of drug dexamethasone without affecting cell viability.					
36095034	5	47	theme	extract	1074:1080	arg1	administration					1052:1065	The administration	1048:1065	The administration of the extract	1048:1080	The administration of the extract significantly inhibited carrageenan-induced paw edema and showed effects similar to those of drug dexamethasone without affecting cell viability.					
36095034	0	48	with	Dereplication	0:12	arg1	activity					78:85	in vivo anti-inflammatory activity	52:85	in vivo anti-inflammatory activity	52:85	Dereplication of Siparuna brasiliensis extract with in vivo anti-inflammatory activity.					
36095034	4	49	theme	elemental	777:785	arg1	composition					787:797	elemental composition	777:797	elemental composition	777:797	From their charge-to-mass ratios (m/z) and elemental composition obtained through Ultrahigh-resolution mass spectrometry analysis by ESI(-)-Orbitrap MS and chromatographic profile by RP-HPLC-PDA, it was possible to annotate polyphenols with anti-inflammatory properties classified as flavonoids and organic acids.					
36095034	2	50	theme	active	351:356	arg1	molecules					358:366	biologically active molecules	338:366	biologically active molecules	338:366	Considering its ethnopharmacological application, it constitutes an important source of biologically active molecules in the development of anti-inflammatory drugs.					
36095034	3	51	theme	inflammatory	711:722	arg1	mediator					724:731	an inflammatory mediator	708:731	an inflammatory mediator	708:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	1	52	theme	indigenous	146:155	arg1	communities					157:167	indigenous communities	146:167	indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies	146:247	Siparuna brasiliensis is a medicinal plant widely used by indigenous communities of the Amazon rainforest to treat inflammatory diseases and related pathologies.					
36095034	3	53	theme	mediator	724:731	arg1	production					694:703	the production	690:703	the production of an inflammatory mediator	690:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	54	from	evaluation	525:534	arg1	brasiliensis					497:508	S. brasiliensis	494:508	S. brasiliensis leaves	494:515	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	54	from	evaluation	525:534	arg1	model					598:602	an in vivo inflammatory model	574:602	an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	574:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	55	theme	inflammatory	585:596	arg1	model					598:602	an in vivo inflammatory model	574:602	an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	574:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	5	56	theme	carrageenan-induced	1106:1124	arg1	edema					1130:1134	carrageenan-induced paw edema	1106:1134	carrageenan-induced paw edema	1106:1134	The administration of the extract significantly inhibited carrageenan-induced paw edema and showed effects similar to those of drug dexamethasone without affecting cell viability.					
36095034	3	57	from	brasiliensis	497:508	arg1	methodology					452:462	a dereplication methodology	436:462	a dereplication methodology of the bioactive extract from S. brasiliensis leaves	436:515	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	57	from	brasiliensis	497:508	arg1	evaluation					525:534	the evaluation	521:534	the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	521:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	57	from	brasiliensis	497:508	arg1	extract					481:487	the bioactive extract	467:487	the bioactive extract from S. brasiliensis leaves	467:515	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
36095034	3	58	theme	in vitro	640:647	arg1	mice					609:612	mice	609:612	mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator	609:731	This study describes a dereplication methodology of the bioactive extract from S. brasiliensis leaves and the evaluation of the anti-inflammatory potential in an in vivo inflammatory model with mice of the BALB/c lineage and in vitro using cell lines, as well as determining the production of an inflammatory mediator.					
35114373	13	0	theme	bone	2150:2153	arg1	regeneration					2155:2166	bone regeneration	2150:2166	bone regeneration	2150:2166	Both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	5	1	from	penetration	748:758	arg1	tube					781:784	the tube	777:784	the tube	777:784	All BMVs supported perfusion and outward penetration of substances in the tube.					
35114373	3	2	theme	alginate-collagen	522:538	arg1	hydrogels					550:558	alginate-collagen composite hydrogels	522:558	alginate-collagen composite hydrogels	522:558	In this study, we have developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	4	3	theme	different	622:630	arg1	sizes					632:636	different sizes	622:636	different sizes	622:636	BMVs with different sizes could be readily prepared by adjusting the flow rate of microfluids.					
35114373	10	4	theme	bone	1566:1569	arg1	regeneration					1571:1582	bone regeneration	1566:1582	bone regeneration	1566:1582	Together, findings from both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	3	5	theme	single	569:574	arg1	step					576:579	a single step	567:579	a single step using microfluidic technology	567:609	In this study, we have developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	12	6	theme	alginate-collagen	1940:1956	arg1	hydrogels					1968:1976	alginate-collagen composite hydrogels	1940:1976	alginate-collagen composite hydrogels	1940:1976	In this study, we developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	8	7	theme	BMSCs	1179:1183	arg1	differentiation					1160:1174	the osteogenic differentiation	1145:1174	the osteogenic differentiation of BMSCs	1145:1183	When co-cultured with bone marrow mesenchymal stem cells (BMSCs), endothelialized BMVs promoted the osteogenic differentiation of BMSCs compared to those in acellular BMV group.					
35114373	7	8	from	cells	993:997	arg1	BMVs					1011:1014	BMVs	1011:1014	BMVs	1011:1014	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	2	9	theme	bone	321:324	arg1	grafts					326:331	bone grafts	321:331	bone grafts	321:331	While various approaches have been tried to build vascular networks in bone grafts, lack of endothelialization still constitutes a major technical hurdle.					
35114373	12	10	theme	single	1987:1992	arg1	step					1994:1997	a single step	1985:1997	a single step using microfluidic technology	1985:2027	In this study, we developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	8	11	dep	marrow	1076:1081	arg1	cells					1100:1104	mesenchymal stem cells	1083:1104	bone marrow mesenchymal stem cells (BMSCs)	1071:1112	When co-cultured with bone marrow mesenchymal stem cells (BMSCs), endothelialized BMVs promoted the osteogenic differentiation of BMSCs compared to those in acellular BMV group.					
35114373	8	11	dep	marrow	1076:1081	arg1	BMSCs					1107:1111	BMSCs	1107:1111	BMSCs	1107:1111	When co-cultured with bone marrow mesenchymal stem cells (BMSCs), endothelialized BMVs promoted the osteogenic differentiation of BMSCs compared to those in acellular BMV group.					
35114373	8	12	theme	BMV	1216:1218	arg1	group					1220:1224	acellular BMV group	1206:1224	acellular BMV group	1206:1224	When co-cultured with bone marrow mesenchymal stem cells (BMSCs), endothelialized BMVs promoted the osteogenic differentiation of BMSCs compared to those in acellular BMV group.					
35114373	9	13	theme	enhanced	1245:1252	arg1	formation					1263:1271	markedly enhanced new bone formation	1236:1271	markedly enhanced new bone formation	1236:1271	In vivo, markedly enhanced new bone formation was achieved by endothelialized BMVs in a rat critical-sized calvarial defect model compared to those with non-endothelialized BMVs or without BMVs.					
35114373	9	14	theme	bone	1258:1261	arg1	formation					1263:1271	markedly enhanced new bone formation	1236:1271	markedly enhanced new bone formation	1236:1271	In vivo, markedly enhanced new bone formation was achieved by endothelialized BMVs in a rat critical-sized calvarial defect model compared to those with non-endothelialized BMVs or without BMVs.					
35114373	2	15	theme	various	256:262	arg1	approaches					264:273	various approaches	256:273	various approaches	256:273	While various approaches have been tried to build vascular networks in bone grafts, lack of endothelialization still constitutes a major technical hurdle.					
35114373	7	16	theme	vein	976:979	arg1	cells					993:997	the rat umbilical vein endothelial cells	958:997	the rat umbilical vein endothelial cells (RUVECs) in BMVs	958:1014	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	7	16	theme	vein	976:979	arg1	RUVECs					1000:1005	RUVECs	1000:1005	RUVECs	1000:1005	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	10	17	theme	effective	1616:1624	arg1	strategy					1626:1633	an effective strategy	1613:1633	an effective strategy in bone tissue engineering	1613:1660	Together, findings from both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	10	18	from	studies	1472:1478	arg1	findings					1432:1439	findings	1432:1439	findings from both in vitro and in vivo studies	1432:1478	Together, findings from both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	0	19	theme	bone	107:110	arg1	repair					112:117	bone repair	107:117	bone repair	107:117	Endothelialized microvessels fabricated by microfluidics facilitate osteogenic differentiation and promote bone repair.					
35114373	5	20	from	tube	781:784	arg1	perfusion					726:734	perfusion	726:734	perfusion	726:734	All BMVs supported perfusion and outward penetration of substances in the tube.					
35114373	5	20	from	tube	781:784	arg1	penetration					748:758	outward penetration	740:758	outward penetration	740:758	All BMVs supported perfusion and outward penetration of substances in the tube.					
35114373	7	21	theme	rat	962:964	arg1	cells					993:997	the rat umbilical vein endothelial cells	958:997	the rat umbilical vein endothelial cells (RUVECs) in BMVs	958:1014	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	7	21	theme	rat	962:964	arg1	RUVECs					1000:1005	RUVECs	1000:1005	RUVECs	1000:1005	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	1	22	theme	critical	178:185	arg1	factors					187:193	the critical factors	174:193	the critical factors	174:193	In bone tissue engineering, vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	13	23	from	strategy	2210:2217	arg1	engineering					2234:2244	bone tissue engineering	2222:2244	bone tissue engineering	2222:2244	Both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	11	24	theme	critical	1756:1763	arg1	factors					1765:1771	the critical factors	1752:1771	the critical factors	1752:1771	STATEMENT OF SIGNIFICANCE: In bone tissue engineering, limited vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	5	25	theme	substances	763:772	arg1	perfusion					726:734	perfusion	726:734	perfusion	726:734	All BMVs supported perfusion and outward penetration of substances in the tube.					
35114373	5	25	theme	substances	763:772	arg1	penetration					748:758	outward penetration	740:758	outward penetration	740:758	All BMVs supported perfusion and outward penetration of substances in the tube.					
35114373	1	26	from	one	167:169	arg1	engineering					135:145	bone tissue engineering	123:145	bone tissue engineering	123:145	In bone tissue engineering, vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	13	27	dep	in	2035:2036	arg1	vitro					2038:2042	vitro	2038:2042	vitro	2038:2042	Both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	10	28	theme	in	1451:1452	arg1	studies					1472:1478	both in vitro and in vivo studies	1446:1478	both in vitro and in vivo studies	1446:1478	Together, findings from both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	10	29	theme	bone	1638:1641	arg1	engineering					1650:1660	bone tissue engineering	1638:1660	bone tissue engineering	1638:1660	Together, findings from both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	0	30	theme	Endothelialized	0:14	arg1	microvessels					16:27	Endothelialized microvessels	0:27	Endothelialized microvessels fabricated by microfluidics	0:55	Endothelialized microvessels fabricated by microfluidics facilitate osteogenic differentiation and promote bone repair.					
35114373	9	31	theme	critical-sized	1319:1332	arg1	model					1351:1355	a rat critical-sized calvarial defect model	1313:1355	a rat critical-sized calvarial defect model	1313:1355	In vivo, markedly enhanced new bone formation was achieved by endothelialized BMVs in a rat critical-sized calvarial defect model compared to those with non-endothelialized BMVs or without BMVs.					
35114373	1	32	theme	tissue	128:133	arg1	engineering					135:145	bone tissue engineering	123:145	bone tissue engineering	123:145	In bone tissue engineering, vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	11	33	theme	tissue	1698:1703	arg1	engineering					1705:1715	bone tissue engineering	1693:1715	bone tissue engineering	1693:1715	STATEMENT OF SIGNIFICANCE: In bone tissue engineering, limited vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	9	34	theme	defect	1344:1349	arg1	model					1351:1355	a rat critical-sized calvarial defect model	1313:1355	a rat critical-sized calvarial defect model	1313:1355	In vivo, markedly enhanced new bone formation was achieved by endothelialized BMVs in a rat critical-sized calvarial defect model compared to those with non-endothelialized BMVs or without BMVs.					
35114373	12	35	theme	microfluidic	2005:2016	arg1	technology					2018:2027	microfluidic technology	2005:2027	microfluidic technology	2005:2027	In this study, we developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	6	36	theme	Endothelial	787:797	arg1	cells					799:803	Endothelial cells	787:803	Endothelial cells	787:803	Endothelial cells could adhere and proliferate on the inner wall of tubes.					
35114373	2	37	theme	endothelialization	342:359	arg1	lack					334:337	lack	334:337	lack of endothelialization	334:359	While various approaches have been tried to build vascular networks in bone grafts, lack of endothelialization still constitutes a major technical hurdle.					
35114373	0	38	theme	osteogenic	68:77	arg1	differentiation					79:93	osteogenic differentiation	68:93	osteogenic differentiation	68:93	Endothelialized microvessels fabricated by microfluidics facilitate osteogenic differentiation and promote bone repair.					
35114373	13	39	theme	bone	2222:2225	arg1	engineering					2234:2244	bone tissue engineering	2222:2244	bone tissue engineering	2222:2244	Both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	8	40	theme	stem	1095:1098	arg1	cells					1100:1104	mesenchymal stem cells	1083:1104	bone marrow mesenchymal stem cells (BMSCs)	1071:1112	When co-cultured with bone marrow mesenchymal stem cells (BMSCs), endothelialized BMVs promoted the osteogenic differentiation of BMSCs compared to those in acellular BMV group.					
35114373	8	40	theme	stem	1095:1098	arg1	BMSCs					1107:1111	BMSCs	1107:1111	BMSCs	1107:1111	When co-cultured with bone marrow mesenchymal stem cells (BMSCs), endothelialized BMVs promoted the osteogenic differentiation of BMSCs compared to those in acellular BMV group.					
35114373	13	41	dep	in	2048:2049	arg1	vivo					2051:2054	vivo	2051:2054	vivo	2051:2054	Both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	3	42	theme	biomimetic	486:495	arg1	BMVs					511:514	BMVs	511:514	BMVs	511:514	In this study, we have developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	3	42	theme	biomimetic	486:495	arg1	microvessels					497:508	endothelialized biomimetic microvessels	470:508	endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels	470:558	In this study, we have developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	7	43	theme	BMP-2	925:929	arg1	secretion					912:920	secretion	912:920	secretion of BMP-2 and PDGF-BB	912:941	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	7	43	theme	BMP-2	925:929	arg1	higher					948:953	higher	948:953	higher	948:953	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	7	43	theme	BMP-2	925:929	arg1	expression					889:898	the expression	885:898	the expression of CD31	885:906	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	1	44	theme	bone	237:240	arg1	repair					242:247	bone repair	237:247	bone repair	237:247	In bone tissue engineering, vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	6	45	theme	tubes	855:859	arg1	wall					847:850	the inner wall	837:850	the inner wall of tubes	837:859	Endothelial cells could adhere and proliferate on the inner wall of tubes.					
35114373	2	46	theme	major	381:385	arg1	hurdle					397:402	a major technical hurdle	379:402	a major technical hurdle	379:402	While various approaches have been tried to build vascular networks in bone grafts, lack of endothelialization still constitutes a major technical hurdle.					
35114373	9	47	theme	non-endothelialized	1380:1398	arg1	BMVs					1400:1403	non-endothelialized BMVs	1380:1403	non-endothelialized BMVs	1380:1403	In vivo, markedly enhanced new bone formation was achieved by endothelialized BMVs in a rat critical-sized calvarial defect model compared to those with non-endothelialized BMVs or without BMVs.					
35114373	9	48	from	BMVs	1305:1308	arg1	model					1351:1355	a rat critical-sized calvarial defect model	1313:1355	a rat critical-sized calvarial defect model	1313:1355	In vivo, markedly enhanced new bone formation was achieved by endothelialized BMVs in a rat critical-sized calvarial defect model compared to those with non-endothelialized BMVs or without BMVs.					
35114373	7	49	theme	CD31	903:906	arg1	secretion					912:920	secretion	912:920	secretion of BMP-2 and PDGF-BB	912:941	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	7	49	theme	CD31	903:906	arg1	higher					948:953	higher	948:953	higher	948:953	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	7	49	theme	CD31	903:906	arg1	expression					889:898	the expression	885:898	the expression of CD31	885:906	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	8	50	theme	endothelialized	1115:1129	arg1	BMVs					1131:1134	endothelialized BMVs	1115:1134	endothelialized BMVs	1115:1134	When co-cultured with bone marrow mesenchymal stem cells (BMSCs), endothelialized BMVs promoted the osteogenic differentiation of BMSCs compared to those in acellular BMV group.					
35114373	6	51	theme	inner	841:845	arg1	wall					847:850	the inner wall	837:850	the inner wall of tubes	837:859	Endothelial cells could adhere and proliferate on the inner wall of tubes.					
35114373	2	52	theme	vascular	300:307	arg1	networks					309:316	vascular networks	300:316	vascular networks in bone grafts	300:331	While various approaches have been tried to build vascular networks in bone grafts, lack of endothelialization still constitutes a major technical hurdle.					
35114373	5	53	from	perfusion	726:734	arg1	tube					781:784	the tube	777:784	the tube	777:784	All BMVs supported perfusion and outward penetration of substances in the tube.					
35114373	11	54	theme	SIGNIFICANCE	1676:1687	arg1	STATEMENT					1663:1671	STATEMENT	1663:1671	STATEMENT OF SIGNIFICANCE	1663:1687	STATEMENT OF SIGNIFICANCE: In bone tissue engineering, limited vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	4	55	theme	microfluids	694:704	arg1	rate					686:689	the flow rate	677:689	the flow rate of microfluids	677:704	BMVs with different sizes could be readily prepared by adjusting the flow rate of microfluids.					
35114373	1	56	theme	factors	187:193	arg1	factors					187:193	the critical factors	174:193	the critical factors	174:193	In bone tissue engineering, vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	1	56	theme	factors	187:193	arg1	one					167:169	one	167:169	one	167:169	In bone tissue engineering, vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	8	57	theme	osteogenic	1149:1158	arg1	differentiation					1160:1174	the osteogenic differentiation	1145:1174	the osteogenic differentiation of BMSCs	1145:1183	When co-cultured with bone marrow mesenchymal stem cells (BMSCs), endothelialized BMVs promoted the osteogenic differentiation of BMSCs compared to those in acellular BMV group.					
35114373	10	58	from	strategy	1626:1633	arg1	engineering					1650:1660	bone tissue engineering	1638:1660	bone tissue engineering	1638:1660	Together, findings from both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	4	59	theme	flow	681:684	arg1	rate					686:689	the flow rate	677:689	the flow rate of microfluids	677:704	BMVs with different sizes could be readily prepared by adjusting the flow rate of microfluids.					
35114373	3	60	theme	microfluidic	587:598	arg1	technology					600:609	microfluidic technology	587:609	microfluidic technology	587:609	In this study, we have developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	12	61	theme	biomimetic	1904:1913	arg1	microvessels					1915:1926	endothelialized biomimetic microvessels	1888:1926	endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels	1888:1976	In this study, we developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	12	61	theme	biomimetic	1904:1913	arg1	BMVs					1929:1932	BMVs	1929:1932	BMVs	1929:1932	In this study, we developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	11	62	theme	biomaterials	1798:1809	arg1	effect					1788:1793	the effect	1784:1793	the effect of biomaterials for bone repair	1784:1825	STATEMENT OF SIGNIFICANCE: In bone tissue engineering, limited vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	5	63	theme	outward	740:746	arg1	penetration					748:758	outward penetration	740:758	outward penetration	740:758	All BMVs supported perfusion and outward penetration of substances in the tube.					
35114373	1	64	theme	biomaterials	220:231	arg1	effect					210:215	the effect	206:215	the effect of biomaterials for bone repair	206:247	In bone tissue engineering, vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	5	65	from	substances	763:772	arg1	tube					781:784	the tube	777:784	the tube	777:784	All BMVs supported perfusion and outward penetration of substances in the tube.					
35114373	8	66	theme	acellular	1206:1214	arg1	group					1220:1224	acellular BMV group	1206:1224	acellular BMV group	1206:1224	When co-cultured with bone marrow mesenchymal stem cells (BMSCs), endothelialized BMVs promoted the osteogenic differentiation of BMSCs compared to those in acellular BMV group.					
35114373	11	67	from	one	1745:1747	arg1	engineering					1705:1715	bone tissue engineering	1693:1715	bone tissue engineering	1693:1715	STATEMENT OF SIGNIFICANCE: In bone tissue engineering, limited vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	11	68	theme	bone	1815:1818	arg1	repair					1820:1825	bone repair	1815:1825	bone repair	1815:1825	STATEMENT OF SIGNIFICANCE: In bone tissue engineering, limited vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	9	69	theme	new	1254:1256	arg1	formation					1263:1271	markedly enhanced new bone formation	1236:1271	markedly enhanced new bone formation	1236:1271	In vivo, markedly enhanced new bone formation was achieved by endothelialized BMVs in a rat critical-sized calvarial defect model compared to those with non-endothelialized BMVs or without BMVs.					
35114373	4	70	with	BMVs	612:615	arg1	sizes					632:636	different sizes	622:636	different sizes	622:636	BMVs with different sizes could be readily prepared by adjusting the flow rate of microfluids.					
35114373	3	71	from	hydrogels	550:558	arg1	BMVs					511:514	BMVs	511:514	BMVs	511:514	In this study, we have developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	3	71	from	hydrogels	550:558	arg1	microvessels					497:508	endothelialized biomimetic microvessels	470:508	endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels	470:558	In this study, we have developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	10	72	dep	in	1464:1465	arg1	vivo					1467:1470	vivo	1467:1470	vivo	1467:1470	Together, findings from both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	7	73	theme	endothelial	981:991	arg1	cells					993:997	the rat umbilical vein endothelial cells	958:997	the rat umbilical vein endothelial cells (RUVECs) in BMVs	958:1014	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	7	73	theme	endothelial	981:991	arg1	RUVECs					1000:1005	RUVECs	1000:1005	RUVECs	1000:1005	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	2	74	from	networks	309:316	arg1	grafts					326:331	bone grafts	321:331	bone grafts	321:331	While various approaches have been tried to build vascular networks in bone grafts, lack of endothelialization still constitutes a major technical hurdle.					
35114373	13	75	theme	endothelialized	2081:2095	arg1	BMVs					2097:2100	endothelialized BMVs	2081:2100	endothelialized BMVs	2081:2100	Both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	7	76	theme	umbilical	966:974	arg1	cells					993:997	the rat umbilical vein endothelial cells	958:997	the rat umbilical vein endothelial cells (RUVECs) in BMVs	958:1014	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	7	76	theme	umbilical	966:974	arg1	RUVECs					1000:1005	RUVECs	1000:1005	RUVECs	1000:1005	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	11	77	theme	factors	1765:1771	arg1	factors					1765:1771	the critical factors	1752:1771	the critical factors	1752:1771	STATEMENT OF SIGNIFICANCE: In bone tissue engineering, limited vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	11	77	theme	factors	1765:1771	arg1	one					1745:1747	one	1745:1747	one	1745:1747	STATEMENT OF SIGNIFICANCE: In bone tissue engineering, limited vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	9	78	theme	endothelialized	1289:1303	arg1	BMVs					1305:1308	endothelialized BMVs	1289:1308	endothelialized BMVs in a rat critical-sized calvarial defect model	1289:1355	In vivo, markedly enhanced new bone formation was achieved by endothelialized BMVs in a rat critical-sized calvarial defect model compared to those with non-endothelialized BMVs or without BMVs.					
35114373	10	79	theme	tissue	1643:1648	arg1	engineering					1650:1660	bone tissue engineering	1638:1660	bone tissue engineering	1638:1660	Together, findings from both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	10	80	dep	in	1451:1452	arg1	vitro					1454:1458	vitro	1454:1458	vitro	1454:1458	Together, findings from both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	12	81	theme	endothelialized	1888:1902	arg1	microvessels					1915:1926	endothelialized biomimetic microvessels	1888:1926	endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels	1888:1976	In this study, we developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	12	81	theme	endothelialized	1888:1902	arg1	BMVs					1929:1932	BMVs	1929:1932	BMVs	1929:1932	In this study, we developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	13	82	theme	in	2048:2049	arg1	studies					2056:2062	Both in vitro and in vivo studies	2030:2062	Both in vitro and in vivo studies	2030:2062	Both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	2	83	theme	technical	387:395	arg1	hurdle					397:402	a major technical hurdle	379:402	a major technical hurdle	379:402	While various approaches have been tried to build vascular networks in bone grafts, lack of endothelialization still constitutes a major technical hurdle.					
35114373	9	84	theme	rat	1315:1317	arg1	model					1351:1355	a rat critical-sized calvarial defect model	1313:1355	a rat critical-sized calvarial defect model	1313:1355	In vivo, markedly enhanced new bone formation was achieved by endothelialized BMVs in a rat critical-sized calvarial defect model compared to those with non-endothelialized BMVs or without BMVs.					
35114373	8	85	theme	bone	1071:1074	arg1	marrow					1076:1081	bone marrow mesenchymal stem cells (BMSCs)	1071:1112	bone marrow mesenchymal stem cells (BMSCs)	1071:1112	When co-cultured with bone marrow mesenchymal stem cells (BMSCs), endothelialized BMVs promoted the osteogenic differentiation of BMSCs compared to those in acellular BMV group.					
35114373	7	86	theme	PDGF-BB	935:941	arg1	secretion					912:920	secretion	912:920	secretion of BMP-2 and PDGF-BB	912:941	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	7	86	theme	PDGF-BB	935:941	arg1	higher					948:953	higher	948:953	higher	948:953	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	7	86	theme	PDGF-BB	935:941	arg1	expression					889:898	the expression	885:898	the expression of CD31	885:906	It was also found that the expression of CD31 and secretion of BMP-2 and PDGF-BB were higher in the rat umbilical vein endothelial cells (RUVECs) in BMVs than those cultured on hydrogel.					
35114373	1	87	theme	bone	123:126	arg1	engineering					135:145	bone tissue engineering	123:145	bone tissue engineering	123:145	In bone tissue engineering, vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	11	88	theme	bone	1693:1696	arg1	engineering					1705:1715	bone tissue engineering	1693:1715	bone tissue engineering	1693:1715	STATEMENT OF SIGNIFICANCE: In bone tissue engineering, limited vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	3	89	theme	endothelialized	470:484	arg1	BMVs					511:514	BMVs	511:514	BMVs	511:514	In this study, we have developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	3	89	theme	endothelialized	470:484	arg1	microvessels					497:508	endothelialized biomimetic microvessels	470:508	endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels	470:558	In this study, we have developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	9	90	theme	calvarial	1334:1342	arg1	model					1351:1355	a rat critical-sized calvarial defect model	1313:1355	a rat critical-sized calvarial defect model	1313:1355	In vivo, markedly enhanced new bone formation was achieved by endothelialized BMVs in a rat critical-sized calvarial defect model compared to those with non-endothelialized BMVs or without BMVs.					
35114373	12	91	theme	facile	1858:1863	arg1	technique					1865:1873	a facile technique	1856:1873	a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology	1856:2027	In this study, we developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	10	92	theme	in	1464:1465	arg1	studies					1472:1478	both in vitro and in vivo studies	1446:1478	both in vitro and in vivo studies	1446:1478	Together, findings from both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	12	93	from	hydrogels	1968:1976	arg1	microvessels					1915:1926	endothelialized biomimetic microvessels	1888:1926	endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels	1888:1976	In this study, we developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	12	93	from	hydrogels	1968:1976	arg1	BMVs					1929:1932	BMVs	1929:1932	BMVs	1929:1932	In this study, we developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	10	94	theme	endothelialized	1497:1511	arg1	BMVs					1513:1516	endothelialized BMVs	1497:1516	endothelialized BMVs	1497:1516	Together, findings from both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	11	95	theme	limited	1718:1724	arg1	vascularization					1726:1740	limited vascularization	1718:1740	limited vascularization	1718:1740	STATEMENT OF SIGNIFICANCE: In bone tissue engineering, limited vascularization is one of the critical factors that limit the effect of biomaterials for bone repair.					
35114373	13	96	theme	in	2035:2036	arg1	studies					2056:2062	Both in vitro and in vivo studies	2030:2062	Both in vitro and in vivo studies	2030:2062	Both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	13	97	theme	tissue	2227:2232	arg1	engineering					2234:2244	bone tissue engineering	2222:2244	bone tissue engineering	2222:2244	Both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
35114373	8	98	theme	mesenchymal	1083:1093	arg1	cells					1100:1104	mesenchymal stem cells	1083:1104	bone marrow mesenchymal stem cells (BMSCs)	1071:1112	When co-cultured with bone marrow mesenchymal stem cells (BMSCs), endothelialized BMVs promoted the osteogenic differentiation of BMSCs compared to those in acellular BMV group.					
35114373	8	98	theme	mesenchymal	1083:1093	arg1	BMSCs					1107:1111	BMSCs	1107:1111	BMSCs	1107:1111	When co-cultured with bone marrow mesenchymal stem cells (BMSCs), endothelialized BMVs promoted the osteogenic differentiation of BMSCs compared to those in acellular BMV group.					
35114373	3	99	theme	facile	440:445	arg1	technique					447:455	a facile technique	438:455	a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology	438:609	In this study, we have developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	12	100	theme	composite	1958:1966	arg1	hydrogels					1968:1976	alginate-collagen composite hydrogels	1940:1976	alginate-collagen composite hydrogels	1940:1976	In this study, we developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	3	101	theme	composite	540:548	arg1	hydrogels					550:558	alginate-collagen composite hydrogels	522:558	alginate-collagen composite hydrogels	522:558	In this study, we have developed a facile technique to fabricate endothelialized biomimetic microvessels (BMVs) from alginate-collagen composite hydrogels within a single step using microfluidic technology.					
35114373	13	102	theme	effective	2200:2208	arg1	strategy					2210:2217	an effective strategy	2197:2217	an effective strategy in bone tissue engineering	2197:2244	Both in vitro and in vivo studies have proven that endothelialized BMVs function to facilitate osteogenesis and promote bone regeneration, and therefore might present an effective strategy in bone tissue engineering.					
36774527	6	0	from	synthesis	1206:1214	arg1	bioprocessing					1232:1244	consolidated bioprocessing	1219:1244	consolidated bioprocessing	1219:1244	These findings offer an overview of resource allocations by N. crassa in response to itaconic acid synthesis and highlight a series of intriguing connections between itaconic acid synthesis and cellulase synthesis in consolidated bioprocessing.					
36774527	3	1	theme	acids	522:526	arg1	synthesis					528:536	fatty acids synthesis	516:536	fatty acids synthesis	516:536	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	6	2	dep	synthesis	1101:1109	arg1	response					1075:1082	response	1075:1082	response	1075:1082	These findings offer an overview of resource allocations by N. crassa in response to itaconic acid synthesis and highlight a series of intriguing connections between itaconic acid synthesis and cellulase synthesis in consolidated bioprocessing.					
36774527	1	3	theme	was	238:240	arg1	354.08 + 35.99 mg/L					242:260	itaconic acid was 354.08 + 35.99 mg/L	224:260	itaconic acid was 354.08 + 35.99 mg/L	224:260	A system for itaconic acid synthesis from cellulose by Neurospora crassa was established, resulting in the highest yield of itaconic acid was 354.08 + 35.99 mg/L.					
36774527	2	4	theme	cellulase	364:372	arg1	production					374:383	improved cellulase production	355:383	improved cellulase production	355:383	Meanwhile, cellulase activity increased significantly, without any strain modifications for improved cellulase production.					
36774527	1	5	theme	354.08 + 35.99 mg/L	242:260	arg1	yield					215:219	the highest yield	203:219	the highest yield of itaconic acid was 354.08 + 35.99 mg/L	203:260	A system for itaconic acid synthesis from cellulose by Neurospora crassa was established, resulting in the highest yield of itaconic acid was 354.08 + 35.99 mg/L.					
36774527	6	6	theme	cellulase	1196:1204	arg1	synthesis					1206:1214	cellulase synthesis	1196:1214	cellulase synthesis	1196:1214	These findings offer an overview of resource allocations by N. crassa in response to itaconic acid synthesis and highlight a series of intriguing connections between itaconic acid synthesis and cellulase synthesis in consolidated bioprocessing.					
36774527	0	7	from	Effect	0:5	arg1	crassa					49:54	Neurospora crassa	38:54	Neurospora crassa in consolidated bioprocessing of cellulose	38:97	Effect of itaconic acid production on Neurospora crassa in consolidated bioprocessing of cellulose.					
36774527	3	8	from	synthesis	528:536	arg1	meiosis					600:606	meiosis	600:606	meiosis	600:606	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	8	from	synthesis	528:536	arg1	pathway					576:582	MAPK signaling pathway	561:582	MAPK signaling pathway	561:582	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	8	from	synthesis	528:536	arg1	cycle					590:594	cell cycle	585:594	cell cycle	585:594	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	9	theme	signaling	566:574	arg1	pathway					576:582	MAPK signaling pathway	561:582	MAPK signaling pathway	561:582	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	10	theme	cell	585:588	arg1	cycle					590:594	cell cycle	585:594	cell cycle	585:594	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	0	11	theme	cellulose	89:97	arg1	bioprocessing					72:84	consolidated bioprocessing	59:84	consolidated bioprocessing of cellulose	59:97	Effect of itaconic acid production on Neurospora crassa in consolidated bioprocessing of cellulose.					
36774527	0	12	from	crassa	49:54	arg1	bioprocessing					72:84	consolidated bioprocessing	59:84	consolidated bioprocessing of cellulose	59:97	Effect of itaconic acid production on Neurospora crassa in consolidated bioprocessing of cellulose.					
36774527	6	13	from	synthesis	1182:1190	arg1	bioprocessing					1232:1244	consolidated bioprocessing	1219:1244	consolidated bioprocessing	1219:1244	These findings offer an overview of resource allocations by N. crassa in response to itaconic acid synthesis and highlight a series of intriguing connections between itaconic acid synthesis and cellulase synthesis in consolidated bioprocessing.					
36774527	5	14	theme	consolidated	974:985	arg1	bioprocessing					987:999	the consolidated bioprocessing	970:999	the consolidated bioprocessing	970:999	Enhancing LPMOs-cellobionic acid/gluconic acid system has the potential to reduce energy consumption of the consolidated bioprocessing.					
36774527	6	15	theme	acid	1096:1099	arg1	synthesis					1101:1109	itaconic acid synthesis	1087:1109	itaconic acid synthesis	1087:1109	These findings offer an overview of resource allocations by N. crassa in response to itaconic acid synthesis and highlight a series of intriguing connections between itaconic acid synthesis and cellulase synthesis in consolidated bioprocessing.					
36774527	4	16	theme	cellulase	732:740	arg1	composition					742:752	the cellulase composition	728:752	the cellulase composition	728:752	More importantly, the low-energy environment enhanced the energy-efficient cellobionic acid/gluconic acid pathway, and the cellulase composition also changed significantly, manifested as the up-regulation of LPMOs and the down-regulation of β-glucosidases.					
36774527	1	17	from	cellulose	142:150	arg1	synthesis					127:135	itaconic acid synthesis	113:135	itaconic acid synthesis from cellulose by Neurospora crassa	113:171	A system for itaconic acid synthesis from cellulose by Neurospora crassa was established, resulting in the highest yield of itaconic acid was 354.08 + 35.99 mg/L.					
36774527	2	18	theme	cellulase	274:282	arg1	activity					284:291	cellulase activity	274:291	cellulase activity	274:291	Meanwhile, cellulase activity increased significantly, without any strain modifications for improved cellulase production.					
36774527	2	18	theme	cellulase	274:282	arg1	Meanwhile					263:271	Meanwhile	263:271	Meanwhile	263:271	Meanwhile, cellulase activity increased significantly, without any strain modifications for improved cellulase production.					
36774527	6	19	theme	itaconic	1168:1175	arg1	synthesis					1182:1190	itaconic acid synthesis	1168:1190	itaconic acid synthesis	1168:1190	These findings offer an overview of resource allocations by N. crassa in response to itaconic acid synthesis and highlight a series of intriguing connections between itaconic acid synthesis and cellulase synthesis in consolidated bioprocessing.					
36774527	4	20	theme	acid	710:713	arg1	pathway					715:721	the energy-efficient cellobionic acid/gluconic acid pathway	663:721	the energy-efficient cellobionic acid/gluconic acid pathway	663:721	More importantly, the low-energy environment enhanced the energy-efficient cellobionic acid/gluconic acid pathway, and the cellulase composition also changed significantly, manifested as the up-regulation of LPMOs and the down-regulation of β-glucosidases.					
36774527	5	21	theme	bioprocessing	987:999	arg1	consumption					955:965	energy consumption	948:965	energy consumption of the consolidated bioprocessing	948:999	Enhancing LPMOs-cellobionic acid/gluconic acid system has the potential to reduce energy consumption of the consolidated bioprocessing.					
36774527	6	22	theme	itaconic	1087:1094	arg1	synthesis					1101:1109	itaconic acid synthesis	1087:1109	itaconic acid synthesis	1087:1109	These findings offer an overview of resource allocations by N. crassa in response to itaconic acid synthesis and highlight a series of intriguing connections between itaconic acid synthesis and cellulase synthesis in consolidated bioprocessing.					
36774527	3	23	theme	MAPK	561:564	arg1	pathway					576:582	MAPK signaling pathway	561:582	MAPK signaling pathway	561:582	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	24	theme	Multi-omics	386:396	arg1	analyses					398:405	Multi-omics analyses	386:405	Multi-omics analyses	386:405	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	4	25	theme	acid/gluconic	696:708	arg1	pathway					715:721	the energy-efficient cellobionic acid/gluconic acid pathway	663:721	the energy-efficient cellobionic acid/gluconic acid pathway	663:721	More importantly, the low-energy environment enhanced the energy-efficient cellobionic acid/gluconic acid pathway, and the cellulase composition also changed significantly, manifested as the up-regulation of LPMOs and the down-regulation of β-glucosidases.					
36774527	5	26	theme	Enhancing	866:874	arg1	system					913:918	Enhancing LPMOs-cellobionic acid/gluconic acid system	866:918	Enhancing LPMOs-cellobionic acid/gluconic acid system	866:918	Enhancing LPMOs-cellobionic acid/gluconic acid system has the potential to reduce energy consumption of the consolidated bioprocessing.					
36774527	6	27	theme	allocations	1047:1057	arg1	overview					1026:1033	an overview	1023:1033	an overview of resource allocations by N. crassa in response to itaconic acid synthesis	1023:1109	These findings offer an overview of resource allocations by N. crassa in response to itaconic acid synthesis and highlight a series of intriguing connections between itaconic acid synthesis and cellulase synthesis in consolidated bioprocessing.					
36774527	3	28	from	wall	510:513	arg1	meiosis					600:606	meiosis	600:606	meiosis	600:606	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	28	from	wall	510:513	arg1	pathway					576:582	MAPK signaling pathway	561:582	MAPK signaling pathway	561:582	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	28	from	wall	510:513	arg1	cycle					590:594	cell cycle	585:594	cell cycle	585:594	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	5	29	theme	energy	948:953	arg1	consumption					955:965	energy consumption	948:965	energy consumption of the consolidated bioprocessing	948:999	Enhancing LPMOs-cellobionic acid/gluconic acid system has the potential to reduce energy consumption of the consolidated bioprocessing.					
36774527	3	30	theme	itaconic	419:426	arg1	synthesis					433:441	itaconic acid synthesis	419:441	itaconic acid synthesis	419:441	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	5	31	contain	has	920:922	arg1	system					913:918	Enhancing LPMOs-cellobionic acid/gluconic acid system	866:918	Enhancing LPMOs-cellobionic acid/gluconic acid system	866:918	Enhancing LPMOs-cellobionic acid/gluconic acid system has the potential to reduce energy consumption of the consolidated bioprocessing.					
36774527	5	31	contain	has	920:922	arg2	potential					928:936	the potential to reduce energy consumption of the consolidated bioprocessing	924:999	the potential to reduce energy consumption of the consolidated bioprocessing	924:999	Enhancing LPMOs-cellobionic acid/gluconic acid system has the potential to reduce energy consumption of the consolidated bioprocessing.					
36774527	0	32	theme	acid	19:22	arg1	production					24:33	itaconic acid production	10:33	itaconic acid production	10:33	Effect of itaconic acid production on Neurospora crassa in consolidated bioprocessing of cellulose.					
36774527	5	33	theme	LPMOs-cellobionic	876:892	arg1	system					913:918	Enhancing LPMOs-cellobionic acid/gluconic acid system	866:918	Enhancing LPMOs-cellobionic acid/gluconic acid system	866:918	Enhancing LPMOs-cellobionic acid/gluconic acid system has the potential to reduce energy consumption of the consolidated bioprocessing.					
36774527	3	34	from	downregulations	542:556	arg1	meiosis					600:606	meiosis	600:606	meiosis	600:606	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	34	from	downregulations	542:556	arg1	pathway					576:582	MAPK signaling pathway	561:582	MAPK signaling pathway	561:582	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	34	from	downregulations	542:556	arg1	cycle					590:594	cell cycle	585:594	cell cycle	585:594	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	4	35	dep	changed	759:765	arg1	manifested					782:791	manifested	782:791	manifested as the up-regulation of LPMOs and the down-regulation of β-glucosidases	782:863	More importantly, the low-energy environment enhanced the energy-efficient cellobionic acid/gluconic acid pathway, and the cellulase composition also changed significantly, manifested as the up-regulation of LPMOs and the down-regulation of β-glucosidases.					
36774527	0	36	theme	itaconic	10:17	arg1	production					24:33	itaconic acid production	10:33	itaconic acid production	10:33	Effect of itaconic acid production on Neurospora crassa in consolidated bioprocessing of cellulose.					
36774527	1	37	theme	Neurospora	155:164	arg1	crassa					166:171	Neurospora crassa	155:171	Neurospora crassa	155:171	A system for itaconic acid synthesis from cellulose by Neurospora crassa was established, resulting in the highest yield of itaconic acid was 354.08 + 35.99 mg/L.					
36774527	3	38	theme	fatty	516:520	arg1	acids					522:526	fatty acids	516:526	fatty acids synthesis	516:536	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	4	39	theme	cellobionic	684:694	arg1	pathway					715:721	the energy-efficient cellobionic acid/gluconic acid pathway	663:721	the energy-efficient cellobionic acid/gluconic acid pathway	663:721	More importantly, the low-energy environment enhanced the energy-efficient cellobionic acid/gluconic acid pathway, and the cellulase composition also changed significantly, manifested as the up-regulation of LPMOs and the down-regulation of β-glucosidases.					
36774527	0	40	theme	production	24:33	arg1	Effect					0:5	Effect	0:5	Effect of itaconic acid production on Neurospora crassa in consolidated bioprocessing of cellulose.	0:98	Effect of itaconic acid production on Neurospora crassa in consolidated bioprocessing of cellulose.					
36774527	6	41	theme	resource	1038:1045	arg1	allocations					1047:1057	resource allocations	1038:1057	resource allocations	1038:1057	These findings offer an overview of resource allocations by N. crassa in response to itaconic acid synthesis and highlight a series of intriguing connections between itaconic acid synthesis and cellulase synthesis in consolidated bioprocessing.					
36774527	3	42	theme	energy	451:456	arg1	production					458:467	energy production	451:467	energy production	451:467	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	5	43	theme	acid/gluconic	894:906	arg1	system					913:918	Enhancing LPMOs-cellobionic acid/gluconic acid system	866:918	Enhancing LPMOs-cellobionic acid/gluconic acid system	866:918	Enhancing LPMOs-cellobionic acid/gluconic acid system has the potential to reduce energy consumption of the consolidated bioprocessing.					
36774527	3	44	from	decreases	481:489	arg1	synthesis					528:536	fatty acids synthesis	516:536	fatty acids synthesis	516:536	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	44	from	decreases	481:489	arg1	trehalose					494:502	trehalose	494:502	trehalose	494:502	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	44	from	decreases	481:489	arg1	wall					510:513	cell wall	505:513	cell wall	505:513	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	44	from	decreases	481:489	arg1	downregulations					542:556	downregulations	542:556	downregulations in MAPK signaling pathway, cell cycle and meiosis	542:606	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	1	45	theme	highest	207:213	arg1	yield					215:219	the highest yield	203:219	the highest yield of itaconic acid was 354.08 + 35.99 mg/L	203:260	A system for itaconic acid synthesis from cellulose by Neurospora crassa was established, resulting in the highest yield of itaconic acid was 354.08 + 35.99 mg/L.					
36774527	3	46	theme	acid	428:431	arg1	synthesis					433:441	itaconic acid synthesis	419:441	itaconic acid synthesis	419:441	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	5	47	theme	acid	908:911	arg1	system					913:918	Enhancing LPMOs-cellobionic acid/gluconic acid system	866:918	Enhancing LPMOs-cellobionic acid/gluconic acid system	866:918	Enhancing LPMOs-cellobionic acid/gluconic acid system has the potential to reduce energy consumption of the consolidated bioprocessing.					
36774527	6	48	theme	N.	1062:1063	arg1	crassa					1065:1070	N. crassa	1062:1070	N. crassa in response to itaconic acid synthesis	1062:1109	These findings offer an overview of resource allocations by N. crassa in response to itaconic acid synthesis and highlight a series of intriguing connections between itaconic acid synthesis and cellulase synthesis in consolidated bioprocessing.					
36774527	4	49	theme	low-energy	631:640	arg1	environment					642:652	the low-energy environment	627:652	the low-energy environment	627:652	More importantly, the low-energy environment enhanced the energy-efficient cellobionic acid/gluconic acid pathway, and the cellulase composition also changed significantly, manifested as the up-regulation of LPMOs and the down-regulation of β-glucosidases.					
36774527	2	50	theme	improved	355:362	arg1	production					374:383	improved cellulase production	355:383	improved cellulase production	355:383	Meanwhile, cellulase activity increased significantly, without any strain modifications for improved cellulase production.					
36774527	0	51	theme	Neurospora	38:47	arg1	crassa					49:54	Neurospora crassa	38:54	Neurospora crassa in consolidated bioprocessing of cellulose	38:97	Effect of itaconic acid production on Neurospora crassa in consolidated bioprocessing of cellulose.					
36774527	3	52	from	trehalose	494:502	arg1	meiosis					600:606	meiosis	600:606	meiosis	600:606	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	52	from	trehalose	494:502	arg1	pathway					576:582	MAPK signaling pathway	561:582	MAPK signaling pathway	561:582	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	3	52	from	trehalose	494:502	arg1	cycle					590:594	cell cycle	585:594	cell cycle	585:594	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	1	53	theme	itaconic	113:120	arg1	synthesis					127:135	itaconic acid synthesis	113:135	itaconic acid synthesis from cellulose by Neurospora crassa	113:171	A system for itaconic acid synthesis from cellulose by Neurospora crassa was established, resulting in the highest yield of itaconic acid was 354.08 + 35.99 mg/L.					
36774527	1	54	theme	acid	122:125	arg1	synthesis					127:135	itaconic acid synthesis	113:135	itaconic acid synthesis from cellulose by Neurospora crassa	113:171	A system for itaconic acid synthesis from cellulose by Neurospora crassa was established, resulting in the highest yield of itaconic acid was 354.08 + 35.99 mg/L.					
36774527	6	55	theme	consolidated	1219:1230	arg1	bioprocessing					1232:1244	consolidated bioprocessing	1219:1244	consolidated bioprocessing	1219:1244	These findings offer an overview of resource allocations by N. crassa in response to itaconic acid synthesis and highlight a series of intriguing connections between itaconic acid synthesis and cellulase synthesis in consolidated bioprocessing.					
36774527	4	56	theme	LPMOs	817:821	arg1	down-regulation					831:845	the down-regulation	827:845	the down-regulation of β-glucosidases	827:863	More importantly, the low-energy environment enhanced the energy-efficient cellobionic acid/gluconic acid pathway, and the cellulase composition also changed significantly, manifested as the up-regulation of LPMOs and the down-regulation of β-glucosidases.					
36774527	4	56	theme	LPMOs	817:821	arg1	up-regulation					800:812	the up-regulation	796:812	the up-regulation of LPMOs	796:821	More importantly, the low-energy environment enhanced the energy-efficient cellobionic acid/gluconic acid pathway, and the cellulase composition also changed significantly, manifested as the up-regulation of LPMOs and the down-regulation of β-glucosidases.					
36774527	2	57	theme	strain	330:335	arg1	modifications					337:349	any strain modifications	326:349	any strain modifications for improved cellulase production	326:383	Meanwhile, cellulase activity increased significantly, without any strain modifications for improved cellulase production.					
36774527	3	58	theme	cell	505:508	arg1	wall					510:513	cell wall	505:513	cell wall	505:513	Multi-omics analyses showed that itaconic acid synthesis reduced energy production, leading to decreases in trehalose, cell wall, fatty acids synthesis and downregulations in MAPK signaling pathway, cell cycle and meiosis.					
36774527	0	59	theme	consolidated	59:70	arg1	bioprocessing					72:84	consolidated bioprocessing	59:84	consolidated bioprocessing of cellulose	59:97	Effect of itaconic acid production on Neurospora crassa in consolidated bioprocessing of cellulose.					
36774527	4	60	theme	energy-efficient	667:682	arg1	pathway					715:721	the energy-efficient cellobionic acid/gluconic acid pathway	663:721	the energy-efficient cellobionic acid/gluconic acid pathway	663:721	More importantly, the low-energy environment enhanced the energy-efficient cellobionic acid/gluconic acid pathway, and the cellulase composition also changed significantly, manifested as the up-regulation of LPMOs and the down-regulation of β-glucosidases.					
36774527	6	61	theme	acid	1177:1180	arg1	synthesis					1182:1190	itaconic acid synthesis	1168:1190	itaconic acid synthesis	1168:1190	These findings offer an overview of resource allocations by N. crassa in response to itaconic acid synthesis and highlight a series of intriguing connections between itaconic acid synthesis and cellulase synthesis in consolidated bioprocessing.					
36774527	1	62	theme	itaconic	224:231	arg1	354.08 + 35.99 mg/L					242:260	itaconic acid was 354.08 + 35.99 mg/L	224:260	itaconic acid was 354.08 + 35.99 mg/L	224:260	A system for itaconic acid synthesis from cellulose by Neurospora crassa was established, resulting in the highest yield of itaconic acid was 354.08 + 35.99 mg/L.					
36774527	4	63	theme	β-glucosidases	850:863	arg1	down-regulation					831:845	the down-regulation	827:845	the down-regulation of β-glucosidases	827:863	More importantly, the low-energy environment enhanced the energy-efficient cellobionic acid/gluconic acid pathway, and the cellulase composition also changed significantly, manifested as the up-regulation of LPMOs and the down-regulation of β-glucosidases.					
36774527	4	63	theme	β-glucosidases	850:863	arg1	up-regulation					800:812	the up-regulation	796:812	the up-regulation of LPMOs	796:821	More importantly, the low-energy environment enhanced the energy-efficient cellobionic acid/gluconic acid pathway, and the cellulase composition also changed significantly, manifested as the up-regulation of LPMOs and the down-regulation of β-glucosidases.					
36774527	1	64	theme	acid	233:236	arg1	354.08 + 35.99 mg/L					242:260	itaconic acid was 354.08 + 35.99 mg/L	224:260	itaconic acid was 354.08 + 35.99 mg/L	224:260	A system for itaconic acid synthesis from cellulose by Neurospora crassa was established, resulting in the highest yield of itaconic acid was 354.08 + 35.99 mg/L.					
36192946	10	0	theme	tested	1306:1311	arg1	formulations					1327:1338	the tested nutraceutical formulations	1302:1338	the tested nutraceutical formulations	1302:1338	The results indicate the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment.					
36192946	1	1	theme	metabolites	541:551	arg1	abundance					462:470	the relative abundance	449:470	the relative abundance of various intestinal bacterial populations	449:514	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	1	theme	metabolites	541:551	arg1	capacity					570:577	antioxidant capacity	558:577	antioxidant capacity	558:577	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	1	theme	metabolites	541:551	arg1	production					517:526	production	517:526	production of microbial metabolites	517:551	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	2	dep	RES	433:435	arg1	150 mg					438:443	150 mg	438:443	150 mg	438:443	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	9	3	theme	fermentation	1243:1254	arg1	media					1256:1260	the colonic fermentation media	1231:1260	the colonic fermentation media	1231:1260	The nutraceutical formulations increased the production of bioactive metabolites and antioxidant capacity in the colonic fermentation media.					
36192946	10	4	theme	intestinal	1483:1492	arg1	environment					1494:1504	the intestinal environment	1479:1504	the intestinal environment	1479:1504	The results indicate the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment.					
36192946	8	5	theme	additive	1042:1049	arg1	interaction					1051:1061	synergistic or additive interaction	1027:1061	synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota	1027:1119	Medium with the formulation containing L. fermentum, QUE, and RES had the highest prebiotic indexes, indicating synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota.					
36192946	1	6	from	impacts	278:284	arg1	abundance					462:470	the relative abundance	449:470	the relative abundance of various intestinal bacterial populations	449:514	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	6	from	impacts	278:284	arg1	capacity					570:577	antioxidant capacity	558:577	antioxidant capacity	558:577	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	6	from	impacts	278:284	arg1	production					517:526	production	517:526	production of microbial metabolites	517:551	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	8	7	theme	prebiotic	997:1005	arg1	indexes					1007:1013	the highest prebiotic indexes	985:1013	the highest prebiotic indexes	985:1013	Medium with the formulation containing L. fermentum, QUE, and RES had the highest prebiotic indexes, indicating synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota.					
36192946	1	8	theme	different	289:297	arg1	formulations					313:324	different nutraceutical formulations	289:324	different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg)	289:444	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	0	9	theme	beneficial	103:112	arg1	impacts					114:120	beneficial impacts	103:120	beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation	103:250	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	0	10	theme	antioxidant	203:213	arg1	capacity					215:222	antioxidant capacity	203:222	antioxidant capacity during colonic fermentation	203:250	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	2	11	theme	relative	670:677	arg1	abundance					679:687	the relative abundance	666:687	the relative abundance of Lactobacillus spp	666:708	The nutraceutical formulations increased the relative abundance of Lactobacillus spp.					
36192946	1	12	theme	nutraceutical	299:311	arg1	formulations					313:324	different nutraceutical formulations	289:324	different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg)	289:444	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	8	13	contain	containing	943:952	arg1	formulation					931:941	the formulation	927:941	the formulation containing L. fermentum, QUE, and RES	927:979	Medium with the formulation containing L. fermentum, QUE, and RES had the highest prebiotic indexes, indicating synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota.					
36192946	8	13	contain	containing	943:952	arg2	QUE					968:970	QUE	968:970	QUE	968:970	Medium with the formulation containing L. fermentum, QUE, and RES had the highest prebiotic indexes, indicating synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota.					
36192946	8	13	contain	containing	943:952	arg2	fermentum					957:965	fermentum	957:965	fermentum	957:965	Medium with the formulation containing L. fermentum, QUE, and RES had the highest prebiotic indexes, indicating synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota.					
36192946	8	13	contain	containing	943:952	arg2	RES					977:979	RES	977:979	RES	977:979	Medium with the formulation containing L. fermentum, QUE, and RES had the highest prebiotic indexes, indicating synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota.					
36192946	0	14	theme	colonic	231:237	arg1	fermentation					239:250	colonic fermentation	231:250	colonic fermentation	231:250	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	8	15	theme	synergistic	1027:1037	arg1	interaction					1051:1061	synergistic or additive interaction	1027:1061	synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota	1027:1119	Medium with the formulation containing L. fermentum, QUE, and RES had the highest prebiotic indexes, indicating synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota.					
36192946	9	16	theme	nutraceutical	1126:1138	arg1	formulations					1140:1151	The nutraceutical formulations	1122:1151	The nutraceutical formulations	1122:1151	The nutraceutical formulations increased the production of bioactive metabolites and antioxidant capacity in the colonic fermentation media.					
36192946	2	17	theme	Lactobacillus	692:704	arg1	spp					706:708	Lactobacillus spp	692:708	Lactobacillus spp	692:708	The nutraceutical formulations increased the relative abundance of Lactobacillus spp.					
36192946	1	18	theme	relative	453:460	arg1	abundance					462:470	the relative abundance	449:470	the relative abundance of various intestinal bacterial populations	449:514	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	19	theme	antioxidant	558:568	arg1	capacity					570:577	antioxidant capacity	558:577	antioxidant capacity	558:577	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	2	20	theme	nutraceutical	629:641	arg1	formulations					643:654	The nutraceutical formulations	625:654	The nutraceutical formulations	625:654	The nutraceutical formulations increased the relative abundance of Lactobacillus spp.					
36192946	5	21	theme	Bacteroides	794:804	arg1	spp					806:808	Bacteroides spp	794:808	Bacteroides spp	794:808	and decreased the relative abundance of Bacteroides spp.					
36192946	0	22	theme	Nutraceutical	0:12	arg1	formulations					14:25	Nutraceutical formulations	0:25	Nutraceutical formulations	0:25	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	1	23	theme	formulations	313:324	arg1	impacts					278:284	the impacts	274:284	the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity	274:577	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	2	24	theme	spp	706:708	arg1	abundance					679:687	the relative abundance	666:687	the relative abundance of Lactobacillus spp	666:708	The nutraceutical formulations increased the relative abundance of Lactobacillus spp.					
36192946	8	25	theme	intestinal	1099:1108	arg1	microbiota					1110:1119	the intestinal microbiota	1095:1119	the intestinal microbiota	1095:1119	Medium with the formulation containing L. fermentum, QUE, and RES had the highest prebiotic indexes, indicating synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota.					
36192946	10	26	theme	microbiota	1427:1436	arg1	composition					1369:1379	composition	1369:1379	composition	1369:1379	The results indicate the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment.					
36192946	10	26	theme	microbiota	1427:1436	arg1	production					1396:1405	metabolite production	1385:1405	metabolite production	1385:1405	The results indicate the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment.					
36192946	0	27	theme	Limosilactobacillus	37:55	arg1	fermentum					57:65	Limosilactobacillus fermentum	37:65	Limosilactobacillus fermentum	37:65	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	1	28	theme	various	475:481	arg1	populations					504:514	various intestinal bacterial populations	475:514	various intestinal bacterial populations	475:514	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	10	29	theme	intestinal	1416:1425	arg1	microbiota					1427:1436	human intestinal microbiota	1410:1436	human intestinal microbiota	1410:1436	The results indicate the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment.					
36192946	10	30	dep	composition	1369:1379	arg1	the					1365:1367	the	1365:1367	the	1365:1367	The results indicate the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment.					
36192946	1	31	theme	Limosilactobacillus	336:354	arg1	CFU/mL					379:384	∼10 log CFU/mL	371:384	∼10 log CFU/mL	371:384	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	31	theme	Limosilactobacillus	336:354	arg1	fermentum					356:364	Limosilactobacillus fermentum 296	336:368	Limosilactobacillus fermentum 296 (∼10 log CFU/mL)	336:385	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	9	32	theme	antioxidant	1207:1217	arg1	capacity					1219:1226	antioxidant capacity	1207:1226	antioxidant capacity	1207:1226	The nutraceutical formulations increased the production of bioactive metabolites and antioxidant capacity in the colonic fermentation media.					
36192946	9	33	theme	bioactive	1181:1189	arg1	metabolites					1191:1201	bioactive metabolites	1181:1201	bioactive metabolites	1181:1201	The nutraceutical formulations increased the production of bioactive metabolites and antioxidant capacity in the colonic fermentation media.					
36192946	10	34	theme	human	1410:1414	arg1	microbiota					1427:1436	human intestinal microbiota	1410:1436	human intestinal microbiota	1410:1436	The results indicate the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment.					
36192946	10	35	from	capacity	1467:1474	arg1	environment					1494:1504	the intestinal environment	1479:1504	the intestinal environment	1479:1504	The results indicate the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment.					
36192946	10	36	theme	metabolite	1385:1394	arg1	production					1396:1405	metabolite production	1385:1405	metabolite production	1385:1405	The results indicate the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment.					
36192946	0	37	theme	bacterial	153:161	arg1	populations					163:173	intestinal bacterial populations	142:173	intestinal bacterial populations	142:173	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	1	38	theme	in	594:595	arg1	fermentation					611:622	in vitro colonic fermentation	594:622	in vitro colonic fermentation	594:622	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	0	39	theme	intestinal	142:151	arg1	populations					163:173	intestinal bacterial populations	142:173	intestinal bacterial populations	142:173	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	1	40	theme	intestinal	483:492	arg1	populations					504:514	various intestinal bacterial populations	475:514	various intestinal bacterial populations	475:514	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	41	dep	QUE	399:401	arg1	160 mg					404:409	160 mg	404:409	160 mg	404:409	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	8	42	contain	had	981:983	arg2	indexes					1007:1013	the highest prebiotic indexes	985:1013	the highest prebiotic indexes	985:1013	Medium with the formulation containing L. fermentum, QUE, and RES had the highest prebiotic indexes, indicating synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota.					
36192946	8	42	contain	had	981:983	arg1	Medium					915:920	Medium	915:920	Medium with the formulation containing L. fermentum, QUE, and RES	915:979	Medium with the formulation containing L. fermentum, QUE, and RES had the highest prebiotic indexes, indicating synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota.					
36192946	8	43	with	Medium	915:920	arg1	formulation					931:941	the formulation	927:941	the formulation containing L. fermentum, QUE, and RES	927:979	Medium with the formulation containing L. fermentum, QUE, and RES had the highest prebiotic indexes, indicating synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota.					
36192946	1	44	theme	or	417:418	arg1	RES					433:435	RES	433:435	RES	433:435	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	44	theme	or	417:418	arg1	resveratrol					420:430	or resveratrol	417:430	or resveratrol (RES, 150 mg)	417:444	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	45	theme	bacterial	494:502	arg1	populations					504:514	various intestinal bacterial populations	475:514	various intestinal bacterial populations	475:514	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	46	theme	∼10	371:373	arg1	CFU/mL					379:384	∼10 log CFU/mL	371:384	∼10 log CFU/mL	371:384	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	46	theme	∼10	371:373	arg1	fermentum					356:364	Limosilactobacillus fermentum 296	336:368	Limosilactobacillus fermentum 296 (∼10 log CFU/mL)	336:385	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	0	47	theme	populations	163:173	arg1	abundance					129:137	the abundance	125:137	the abundance of intestinal bacterial populations	125:173	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	0	47	theme	populations	163:173	arg1	capacity					215:222	antioxidant capacity	203:222	antioxidant capacity during colonic fermentation	203:250	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	0	47	theme	populations	163:173	arg1	production					187:196	metabolite production	176:196	metabolite production	176:196	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	1	48	theme	populations	504:514	arg1	abundance					462:470	the relative abundance	449:470	the relative abundance of various intestinal bacterial populations	449:514	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	48	theme	populations	504:514	arg1	capacity					570:577	antioxidant capacity	558:577	antioxidant capacity	558:577	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	48	theme	populations	504:514	arg1	production					517:526	production	517:526	production of microbial metabolites	517:551	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	10	49	theme	formulations	1327:1338	arg1	capability					1288:1297	the capability	1284:1297	the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment	1284:1504	The results indicate the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment.					
36192946	1	50	theme	log	375:377	arg1	CFU/mL					379:384	∼10 log CFU/mL	371:384	∼10 log CFU/mL	371:384	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	1	50	theme	log	375:377	arg1	fermentum					356:364	Limosilactobacillus fermentum 296	336:368	Limosilactobacillus fermentum 296 (∼10 log CFU/mL)	336:385	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	9	51	theme	colonic	1235:1241	arg1	media					1256:1260	the colonic fermentation media	1231:1260	the colonic fermentation media	1231:1260	The nutraceutical formulations increased the production of bioactive metabolites and antioxidant capacity in the colonic fermentation media.					
36192946	9	52	theme	capacity	1219:1226	arg1	production					1167:1176	the production	1163:1176	the production of bioactive metabolites and antioxidant capacity	1163:1226	The nutraceutical formulations increased the production of bioactive metabolites and antioxidant capacity in the colonic fermentation media.					
36192946	1	53	theme	colonic	603:609	arg1	fermentation					611:622	in vitro colonic fermentation	594:622	in vitro colonic fermentation	594:622	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	0	54	theme	metabolite	176:185	arg1	production					187:196	metabolite production	176:196	metabolite production	176:196	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	0	55	from	impacts	114:120	arg1	abundance					129:137	the abundance	125:137	the abundance of intestinal bacterial populations	125:173	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	0	55	from	impacts	114:120	arg1	capacity					215:222	antioxidant capacity	203:222	antioxidant capacity during colonic fermentation	203:250	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	0	55	from	impacts	114:120	arg1	production					187:196	metabolite production	176:196	metabolite production	176:196	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	9	56	theme	metabolites	1191:1201	arg1	production					1167:1176	the production	1163:1176	the production of bioactive metabolites and antioxidant capacity	1163:1226	The nutraceutical formulations increased the production of bioactive metabolites and antioxidant capacity in the colonic fermentation media.					
36192946	8	57	theme	highest	989:995	arg1	indexes					1007:1013	the highest prebiotic indexes	985:1013	the highest prebiotic indexes	985:1013	Medium with the formulation containing L. fermentum, QUE, and RES had the highest prebiotic indexes, indicating synergistic or additive interaction between QUE and RES to modulate the intestinal microbiota.					
36192946	1	58	theme	fermentation	611:622	arg1	48 h					586:589	48 h	586:589	48 h of in vitro colonic fermentation	586:622	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	7	59	theme	colonic	893:899	arg1	fermentation					901:912	the colonic fermentation	889:912	the colonic fermentation	889:912	coccoides during the colonic fermentation.					
36192946	0	60	theme	or	83:84	arg1	resveratrol					86:96	or resveratrol	83:96	or resveratrol	83:96	Nutraceutical formulations combining Limosilactobacillus fermentum, quercetin, and or resveratrol with beneficial impacts on the abundance of intestinal bacterial populations, metabolite production, and antioxidant capacity during colonic fermentation.					
36192946	1	61	dep	in	594:595	arg1	vitro					597:601	vitro	597:601	vitro	597:601	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	10	62	theme	nutraceutical	1313:1325	arg1	formulations					1327:1338	the tested nutraceutical formulations	1302:1338	the tested nutraceutical formulations	1302:1338	The results indicate the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment.					
36192946	5	63	theme	spp	806:808	arg1	abundance					781:789	the relative abundance	768:789	the relative abundance of Bacteroides spp	768:808	and decreased the relative abundance of Bacteroides spp.					
36192946	1	64	theme	microbial	531:539	arg1	metabolites					541:551	microbial metabolites	531:551	microbial metabolites	531:551	This study evaluated the impacts of different nutraceutical formulations combining Limosilactobacillus fermentum 296 (∼10 log CFU/mL), quercetin (QUE, 160 mg), and or resveratrol (RES, 150 mg) on the relative abundance of various intestinal bacterial populations, production of microbial metabolites, and antioxidant capacity during 48 h of in vitro colonic fermentation.					
36192946	10	65	theme	antioxidant	1455:1465	arg1	capacity					1467:1474	the antioxidant capacity	1451:1474	the antioxidant capacity in the intestinal environment	1451:1504	The results indicate the capability of the tested nutraceutical formulations to beneficially modulate the composition and metabolite production of human intestinal microbiota and increase the antioxidant capacity in the intestinal environment.					
36192946	5	66	theme	relative	772:779	arg1	abundance					781:789	the relative abundance	768:789	the relative abundance of Bacteroides spp	768:808	and decreased the relative abundance of Bacteroides spp.					
37392633	2	0	theme	infusion	563:570	arg1	comparison					480:489	comparison	480:489	comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles	480:592	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	5	1	theme	phenolic	1020:1027	arg1	content					1029:1035	the total phenolic content	1010:1035	the total phenolic content of 7.63 μg/mL	1010:1049	A total of 23 individual phenolic compounds were identified in submicroparticles with the total phenolic content of 7.63 μg/mL.					
37392633	2	2	from	infusion	468:475	arg1	absorption					423:432	the intestinal absorption	408:432	the intestinal absorption of phenolics in chrysanthemum tea infusion	408:475	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	2	3	theme	tea	559:561	arg1	infusion					563:570	submicroparticles-free chrysanthemum tea infusion	522:570	submicroparticles-free chrysanthemum tea infusion	522:570	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	4	4	with	pectin	831:836	arg1	conformation					855:866	a spherical conformation	843:866	a spherical conformation	843:866	The polysaccharide, which was determined as esterified pectin with a spherical conformation, provided spherical skeleton to form submicroparticles.					
37392633	5	5	theme	7.63 μg/mL	1040:1049	arg1	content					1029:1035	the total phenolic content	1010:1035	the total phenolic content of 7.63 μg/mL	1010:1049	A total of 23 individual phenolic compounds were identified in submicroparticles with the total phenolic content of 7.63 μg/mL.					
37392633	6	6	theme	hydrogen	1130:1137	arg1	bonds					1139:1143	hydrogen bonds	1130:1143	hydrogen bonds	1130:1143	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	4	7	theme	spherical	878:886	arg1	skeleton					888:895	spherical skeleton	878:895	spherical skeleton	878:895	The polysaccharide, which was determined as esterified pectin with a spherical conformation, provided spherical skeleton to form submicroparticles.					
37392633	6	8	theme	pectin	1120:1125	arg1	region					1100:1105	the external region	1087:1105	the external region of spherical pectin	1087:1125	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	6	9	attach	attached	1209:1216	arg2	phenolics					1056:1064	The phenolics	1052:1064	The phenolics	1052:1064	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	6	9	attach	attached	1209:1216	arg1	region					1234:1239	the internal region	1221:1239	the internal region	1221:1239	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	1	10	dep	components	135:144	arg1	existed					156:162	existed	156:162	existed in chrysanthemum tea infusion	156:192	Submicroparticles are important components generally existed in chrysanthemum tea infusion, but their functionality, chemical composition, structure and self-assembly mechanism are unclear due to lack of suitable preparation method and research strategy.					
37392633	2	11	theme	infusion	512:519	arg1	comparison					480:489	comparison	480:489	comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles	480:592	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	0	12	theme	Chemical	0:7	arg1	basis					9:13	Chemical basis	0:13	Chemical basis	0:13	Chemical basis and self-assembly mechanism of submicroparticles forming in chrysanthemum tea infusion.					
37392633	2	13	theme	phenolics	437:445	arg1	absorption					423:432	the intestinal absorption	408:432	the intestinal absorption of phenolics in chrysanthemum tea infusion	408:475	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	2	14	theme	tea	508:510	arg1	infusion					512:519	chrysanthemum tea infusion	494:519	chrysanthemum tea infusion	494:519	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	0	15	theme	tea	89:91	arg1	infusion					93:100	chrysanthemum tea infusion	75:100	chrysanthemum tea infusion	75:100	Chemical basis and self-assembly mechanism of submicroparticles forming in chrysanthemum tea infusion.					
37392633	2	16	theme	submicroparticles	576:592	arg1	comparison					480:489	comparison	480:489	comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles	480:592	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	3	17	from	solids	738:743	arg1	infusion					766:773	chrysanthemum tea infusion	748:773	chrysanthemum tea infusion	748:773	Submicroparticles efficiently prepared by ultrafiltration mainly consisting of polysaccharide and phenolics accounted for 22% of total soluble solids in chrysanthemum tea infusion.					
37392633	6	18	theme	pectin	1198:1203	arg1	cavities					1176:1183	hydrophobic cavities	1164:1183	hydrophobic cavities of spherical pectin	1164:1203	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	2	19	theme	chrysanthemum	494:506	arg1	infusion					512:519	chrysanthemum tea infusion	494:519	chrysanthemum tea infusion	494:519	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	6	20	theme	spherical	1110:1118	arg1	pectin					1120:1125	spherical pectin	1110:1125	spherical pectin	1110:1125	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	4	21	theme	spherical	845:853	arg1	conformation					855:866	a spherical conformation	843:866	a spherical conformation	843:866	The polysaccharide, which was determined as esterified pectin with a spherical conformation, provided spherical skeleton to form submicroparticles.					
37392633	5	22	theme	total	1014:1018	arg1	content					1029:1035	the total phenolic content	1010:1035	the total phenolic content of 7.63 μg/mL	1010:1049	A total of 23 individual phenolic compounds were identified in submicroparticles with the total phenolic content of 7.63 μg/mL.					
37392633	0	23	theme	self-assembly	19:31	arg1	mechanism					33:41	self-assembly mechanism	19:41	self-assembly mechanism	19:41	Chemical basis and self-assembly mechanism of submicroparticles forming in chrysanthemum tea infusion.					
37392633	1	24	theme	suitable	307:314	arg1	method					328:333	suitable preparation method	307:333	suitable preparation method	307:333	Submicroparticles are important components generally existed in chrysanthemum tea infusion, but their functionality, chemical composition, structure and self-assembly mechanism are unclear due to lack of suitable preparation method and research strategy.					
37392633	1	25	theme	chemical	220:227	arg1	composition					229:239	chemical composition	220:239	chemical composition	220:239	Submicroparticles are important components generally existed in chrysanthemum tea infusion, but their functionality, chemical composition, structure and self-assembly mechanism are unclear due to lack of suitable preparation method and research strategy.					
37392633	5	26	theme	phenolic	949:956	arg1	compounds					958:966	23 individual phenolic compounds	935:966	23 individual phenolic compounds	935:966	A total of 23 individual phenolic compounds were identified in submicroparticles with the total phenolic content of 7.63 μg/mL.					
37392633	1	27	theme	preparation	316:326	arg1	method					328:333	suitable preparation method	307:333	suitable preparation method	307:333	Submicroparticles are important components generally existed in chrysanthemum tea infusion, but their functionality, chemical composition, structure and self-assembly mechanism are unclear due to lack of suitable preparation method and research strategy.					
37392633	3	28	theme	tea	762:764	arg1	infusion					766:773	chrysanthemum tea infusion	748:773	chrysanthemum tea infusion	748:773	Submicroparticles efficiently prepared by ultrafiltration mainly consisting of polysaccharide and phenolics accounted for 22% of total soluble solids in chrysanthemum tea infusion.					
37392633	1	29	theme	important	125:133	arg1	Submicroparticles					103:119	Submicroparticles	103:119	Submicroparticles	103:119	Submicroparticles are important components generally existed in chrysanthemum tea infusion, but their functionality, chemical composition, structure and self-assembly mechanism are unclear due to lack of suitable preparation method and research strategy.					
37392633	1	29	theme	important	125:133	arg1	components					135:144	important components	125:144	important components	125:144	Submicroparticles are important components generally existed in chrysanthemum tea infusion, but their functionality, chemical composition, structure and self-assembly mechanism are unclear due to lack of suitable preparation method and research strategy.					
37392633	2	30	theme	intestinal	412:421	arg1	absorption					423:432	the intestinal absorption	408:432	the intestinal absorption of phenolics in chrysanthemum tea infusion	408:475	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	2	31	theme	chrysanthemum	545:557	arg1	infusion					563:570	submicroparticles-free chrysanthemum tea infusion	522:570	submicroparticles-free chrysanthemum tea infusion	522:570	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	1	32	theme	method	328:333	arg1	lack					299:302	lack	299:302	lack of suitable preparation method and research strategy	299:355	Submicroparticles are important components generally existed in chrysanthemum tea infusion, but their functionality, chemical composition, structure and self-assembly mechanism are unclear due to lack of suitable preparation method and research strategy.					
37392633	6	33	theme	hydrophobic	1164:1174	arg1	cavities					1176:1183	hydrophobic cavities	1164:1183	hydrophobic cavities of spherical pectin	1164:1203	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	6	34	theme	external	1091:1098	arg1	region					1100:1105	the external region	1087:1105	the external region of spherical pectin	1087:1125	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	3	35	from	%	719:719	arg1	infusion					766:773	chrysanthemum tea infusion	748:773	chrysanthemum tea infusion	748:773	Submicroparticles efficiently prepared by ultrafiltration mainly consisting of polysaccharide and phenolics accounted for 22% of total soluble solids in chrysanthemum tea infusion.					
37392633	2	36	theme	tea	464:466	arg1	infusion					468:475	chrysanthemum tea infusion	450:475	chrysanthemum tea infusion	450:475	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	2	37	theme	submicroparticles-free	522:543	arg1	infusion					563:570	submicroparticles-free chrysanthemum tea infusion	522:570	submicroparticles-free chrysanthemum tea infusion	522:570	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	5	38	theme	individual	938:947	arg1	compounds					958:966	23 individual phenolic compounds	935:966	23 individual phenolic compounds	935:966	A total of 23 individual phenolic compounds were identified in submicroparticles with the total phenolic content of 7.63 μg/mL.					
37392633	2	39	theme	chrysanthemum	450:462	arg1	infusion					468:475	chrysanthemum tea infusion	450:475	chrysanthemum tea infusion	450:475	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	1	40	theme	research	339:346	arg1	strategy					348:355	research strategy	339:355	research strategy	339:355	Submicroparticles are important components generally existed in chrysanthemum tea infusion, but their functionality, chemical composition, structure and self-assembly mechanism are unclear due to lack of suitable preparation method and research strategy.					
37392633	3	41	theme	chrysanthemum	748:760	arg1	infusion					766:773	chrysanthemum tea infusion	748:773	chrysanthemum tea infusion	748:773	Submicroparticles efficiently prepared by ultrafiltration mainly consisting of polysaccharide and phenolics accounted for 22% of total soluble solids in chrysanthemum tea infusion.					
37392633	2	42	from	absorption	423:432	arg1	infusion					468:475	chrysanthemum tea infusion	450:475	chrysanthemum tea infusion	450:475	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	0	43	theme	submicroparticles	46:62	arg1	basis					9:13	Chemical basis	0:13	Chemical basis	0:13	Chemical basis and self-assembly mechanism of submicroparticles forming in chrysanthemum tea infusion.					
37392633	0	43	theme	submicroparticles	46:62	arg1	mechanism					33:41	self-assembly mechanism	19:41	self-assembly mechanism	19:41	Chemical basis and self-assembly mechanism of submicroparticles forming in chrysanthemum tea infusion.					
37392633	1	44	theme	strategy	348:355	arg1	lack					299:302	lack	299:302	lack of suitable preparation method and research strategy	299:355	Submicroparticles are important components generally existed in chrysanthemum tea infusion, but their functionality, chemical composition, structure and self-assembly mechanism are unclear due to lack of suitable preparation method and research strategy.					
37392633	6	45	theme	spherical	1188:1196	arg1	pectin					1198:1203	spherical pectin	1188:1203	spherical pectin	1188:1203	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	6	46	attach	attached	1075:1082	arg1	region					1100:1105	the external region	1087:1105	the external region of spherical pectin	1087:1125	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	6	46	attach	attached	1075:1082	arg2	phenolics					1056:1064	The phenolics	1052:1064	The phenolics	1052:1064	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	4	47	theme	esterified	820:829	arg1	pectin					831:836	esterified pectin	820:836	esterified pectin with a spherical conformation	820:866	The polysaccharide, which was determined as esterified pectin with a spherical conformation, provided spherical skeleton to form submicroparticles.					
37392633	4	47	theme	esterified	820:829	arg1	polysaccharide					780:793	The polysaccharide	776:793	The polysaccharide	776:793	The polysaccharide, which was determined as esterified pectin with a spherical conformation, provided spherical skeleton to form submicroparticles.					
37392633	3	48	from	infusion	766:773	arg1	%					719:719	22%	717:719	22% of total soluble solids in chrysanthemum tea infusion	717:773	Submicroparticles efficiently prepared by ultrafiltration mainly consisting of polysaccharide and phenolics accounted for 22% of total soluble solids in chrysanthemum tea infusion.					
37392633	3	48	from	infusion	766:773	arg1	solids					738:743	total soluble solids	724:743	total soluble solids in chrysanthemum tea infusion	724:773	Submicroparticles efficiently prepared by ultrafiltration mainly consisting of polysaccharide and phenolics accounted for 22% of total soluble solids in chrysanthemum tea infusion.					
37392633	5	49	with	submicroparticles	987:1003	arg1	content					1029:1035	the total phenolic content	1010:1035	the total phenolic content of 7.63 μg/mL	1010:1049	A total of 23 individual phenolic compounds were identified in submicroparticles with the total phenolic content of 7.63 μg/mL.					
37392633	3	50	theme	soluble	730:736	arg1	solids					738:743	total soluble solids	724:743	total soluble solids in chrysanthemum tea infusion	724:773	Submicroparticles efficiently prepared by ultrafiltration mainly consisting of polysaccharide and phenolics accounted for 22% of total soluble solids in chrysanthemum tea infusion.					
37392633	2	51	from	phenolics	437:445	arg1	infusion					468:475	chrysanthemum tea infusion	450:475	chrysanthemum tea infusion	450:475	This study showed that submicroparticles promoted the intestinal absorption of phenolics in chrysanthemum tea infusion by comparison of chrysanthemum tea infusion, submicroparticles-free chrysanthemum tea infusion and submicroparticles.					
37392633	6	52	theme	hydrophobic	1244:1254	arg1	interactions					1256:1267	hydrophobic interactions	1244:1267	hydrophobic interactions	1244:1267	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	3	53	theme	solids	738:743	arg1	%					719:719	22%	717:719	22% of total soluble solids in chrysanthemum tea infusion	717:773	Submicroparticles efficiently prepared by ultrafiltration mainly consisting of polysaccharide and phenolics accounted for 22% of total soluble solids in chrysanthemum tea infusion.					
37392633	3	53	theme	solids	738:743	arg1	solids					738:743	total soluble solids	724:743	total soluble solids in chrysanthemum tea infusion	724:773	Submicroparticles efficiently prepared by ultrafiltration mainly consisting of polysaccharide and phenolics accounted for 22% of total soluble solids in chrysanthemum tea infusion.					
37392633	6	54	theme	internal	1225:1232	arg1	region					1234:1239	the internal region	1221:1239	the internal region	1221:1239	The phenolics not only attached to the external region of spherical pectin by hydrogen bonds, but also got into hydrophobic cavities of spherical pectin and attached to the internal region by hydrophobic interactions.					
37392633	1	55	theme	self-assembly	256:268	arg1	mechanism					270:278	self-assembly mechanism	256:278	self-assembly mechanism	256:278	Submicroparticles are important components generally existed in chrysanthemum tea infusion, but their functionality, chemical composition, structure and self-assembly mechanism are unclear due to lack of suitable preparation method and research strategy.					
37392633	0	56	theme	chrysanthemum	75:87	arg1	infusion					93:100	chrysanthemum tea infusion	75:100	chrysanthemum tea infusion	75:100	Chemical basis and self-assembly mechanism of submicroparticles forming in chrysanthemum tea infusion.					
37392633	5	57	theme	compounds	958:966	arg1	total					926:930	A total	924:930	A total of 23 individual phenolic compounds	924:966	A total of 23 individual phenolic compounds were identified in submicroparticles with the total phenolic content of 7.63 μg/mL.					
37392633	1	58	theme	chrysanthemum	167:179	arg1	infusion					185:192	chrysanthemum tea infusion	167:192	chrysanthemum tea infusion	167:192	Submicroparticles are important components generally existed in chrysanthemum tea infusion, but their functionality, chemical composition, structure and self-assembly mechanism are unclear due to lack of suitable preparation method and research strategy.					
37392633	3	59	theme	total	724:728	arg1	solids					738:743	total soluble solids	724:743	total soluble solids in chrysanthemum tea infusion	724:773	Submicroparticles efficiently prepared by ultrafiltration mainly consisting of polysaccharide and phenolics accounted for 22% of total soluble solids in chrysanthemum tea infusion.					
37392633	1	60	theme	tea	181:183	arg1	infusion					185:192	chrysanthemum tea infusion	167:192	chrysanthemum tea infusion	167:192	Submicroparticles are important components generally existed in chrysanthemum tea infusion, but their functionality, chemical composition, structure and self-assembly mechanism are unclear due to lack of suitable preparation method and research strategy.					
35450620	0	0	theme	wound	106:110	arg1	healing					112:118	wound healing	106:118	wound healing	106:118	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel incorporated with bioglass nanoparticles for wound healing.					
35450620	2	1	theme	well-defined	480:491	arg1	elasticity					493:502	elasticity	493:502	elasticity	493:502	The optimally achieved gels showed greatly enhanced strength and stiffness compared to the gels built from either THA or SF while having well-defined elasticity and injectability.					
35450620	2	2	theme	achieved	357:364	arg1	gels					366:369	The optimally achieved gels	343:369	The optimally achieved gels	343:369	The optimally achieved gels showed greatly enhanced strength and stiffness compared to the gels built from either THA or SF while having well-defined elasticity and injectability.					
35450620	1	3	theme	bioactive	240:248	arg1	nanoparticles					256:268	bioactive glass nanoparticles	240:268	bioactive glass nanoparticles	240:268	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	1	4	with	hydrogel	305:312	arg1	structure					332:340	dual-network structure	319:340	dual-network structure	319:340	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	5	5	theme	skin	904:907	arg1	defect					909:914	the 10 mm mouse full-thickness skin defect	873:914	the 10 mm mouse full-thickness skin defect	873:914	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	1	6	theme	glass	250:254	arg1	nanoparticles					256:268	bioactive glass nanoparticles	240:268	bioactive glass nanoparticles	240:268	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	4	7	theme	endothelial	829:839	arg1	cells					841:845	human umbilical vein endothelial cells	808:845	human umbilical vein endothelial cells	808:845	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	5	8	theme	full-thickness	889:902	arg1	defect					909:914	the 10 mm mouse full-thickness skin defect	873:914	the 10 mm mouse full-thickness skin defect	873:914	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	4	9	theme	cells	718:722	arg1	proliferation					694:706	proliferation	694:706	proliferation	694:706	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	4	9	theme	cells	718:722	arg1	in-growth					680:688	in-growth	680:688	in-growth	680:688	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	2	10	dep	showed	371:376	arg1	compared					418:425	compared	418:425	showed greatly enhanced strength and stiffness compared to the gels built from either THA or SF while having well-defined elasticity and injectability	371:520	The optimally achieved gels showed greatly enhanced strength and stiffness compared to the gels built from either THA or SF while having well-defined elasticity and injectability.					
35450620	4	11	theme	cells	841:845	arg1	migration					774:782	the migration	770:782	the migration of both fibroblasts and human umbilical vein endothelial cells	770:845	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	4	12	dep	in-growth	680:688	arg1	the					676:678	the	676:678	the	676:678	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	1	13	theme	selected	188:195	arg1	THA					197:199	the selected THA	184:199	the selected THA	184:199	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	0	14	theme	hyaluronic	10:19	arg1	hydrogel					52:59	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel	0:59	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel incorporated with bioglass	0:86	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel incorporated with bioglass nanoparticles for wound healing.					
35450620	2	15	theme	enhanced	386:393	arg1	strength					395:402	greatly enhanced strength	378:402	greatly enhanced strength	378:402	The optimally achieved gels showed greatly enhanced strength and stiffness compared to the gels built from either THA or SF while having well-defined elasticity and injectability.					
35450620	0	16	theme	Thiolated	0:8	arg1	hydrogel					52:59	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel	0:59	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel incorporated with bioglass	0:86	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel incorporated with bioglass nanoparticles for wound healing.					
35450620	0	17	theme	fibroin	31:37	arg1	hydrogel					52:59	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel	0:59	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel incorporated with bioglass	0:86	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel incorporated with bioglass nanoparticles for wound healing.					
35450620	5	18	theme	appendages	1035:1044	arg1	formation					988:996	formation	988:996	formation of vascularized tissues and complete appendages	988:1044	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	1	19	theme	Thiolated	121:129	arg1	conjugates					152:161	Thiolated hyaluronic acid(THA) conjugates	121:161	Thiolated hyaluronic acid(THA) conjugates	121:161	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	5	20	theme	tissues	1014:1020	arg1	formation					988:996	formation	988:996	formation of vascularized tissues and complete appendages	988:1044	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	4	21	theme	cell	634:637	arg1	culture					639:645	In vitro cell culture	625:645	In vitro cell culture	625:645	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	0	22	theme	acid/silk	21:29	arg1	hydrogel					52:59	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel	0:59	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel incorporated with bioglass	0:86	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel incorporated with bioglass nanoparticles for wound healing.					
35450620	1	23	theme	novel	281:285	arg1	type					287:290	a novel type	279:290	a novel type of composite hydrogel with dual-network structure	279:340	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	5	24	theme	skin	970:973	arg1	defects					975:981	the skin defects	966:981	the skin defects with formation of vascularized tissues and complete appendages	966:1044	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	1	25	theme	hyaluronic	131:140	arg1	THA					147:149	THA	147:149	THA	147:149	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	1	25	theme	hyaluronic	131:140	arg1	acid					142:145	hyaluronic acid	131:145	Thiolated hyaluronic acid(THA) conjugates	121:161	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	5	26	theme	complete	1026:1033	arg1	appendages					1035:1044	complete appendages	1026:1044	complete appendages	1026:1044	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	5	27	theme	In	848:849	arg1	results					856:862	In vivo results	848:862	In vivo results based on the 10 mm mouse full-thickness skin defect	848:914	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	4	28	theme	umbilical	814:822	arg1	cells					841:845	human umbilical vein endothelial cells	808:845	human umbilical vein endothelial cells	808:845	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	1	29	theme	silk	219:222	arg1	SF					232:233	SF	232:233	SF	232:233	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	1	29	theme	silk	219:222	arg1	fibroin					224:230	silk fibroin	219:230	silk fibroin(SF)	219:234	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	0	30	theme	dual-network	39:50	arg1	hydrogel					52:59	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel	0:59	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel incorporated with bioglass	0:86	Thiolated hyaluronic acid/silk fibroin dual-network hydrogel incorporated with bioglass nanoparticles for wound healing.					
35450620	5	31	with	defects	975:981	arg1	formation					988:996	formation	988:996	formation of vascularized tissues and complete appendages	988:1044	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	4	32	theme	human	808:812	arg1	cells					841:845	human umbilical vein endothelial cells	808:845	human umbilical vein endothelial cells	808:845	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	1	33	theme	composite	295:303	arg1	hydrogel					305:312	composite hydrogel	295:312	composite hydrogel with dual-network structure	295:340	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	5	34	theme	promising	1088:1096	arg1	potency					1098:1104	their highly promising potency	1075:1104	their highly promising potency in use for wound healing	1075:1129	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	3	35	theme	linear	586:591	arg1	manners					593:599	approximately linear manners	572:599	approximately linear manners	572:599	They were able to sustainably release Si ions in approximately linear manners for around three weeks.					
35450620	1	36	theme	acid	142:145	arg1	conjugates					152:161	Thiolated hyaluronic acid(THA) conjugates	121:161	Thiolated hyaluronic acid(THA) conjugates	121:161	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	1	37	theme	hydrogel	305:312	arg1	type					287:290	a novel type	279:290	a novel type of composite hydrogel with dual-network structure	279:340	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	5	38	theme	wound	1117:1121	arg1	healing					1123:1129	wound healing	1117:1129	wound healing	1117:1129	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	4	39	dep	In	625:626	arg1	vitro					628:632	vitro	628:632	vitro	628:632	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	4	40	theme	vein	824:827	arg1	cells					841:845	human umbilical vein endothelial cells	808:845	human umbilical vein endothelial cells	808:845	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	4	41	theme	seeded	711:716	arg1	cells					718:722	seeded cells	711:722	seeded cells	711:722	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	5	42	theme	vascularized	1001:1012	arg1	tissues					1014:1020	vascularized tissues	1001:1020	vascularized tissues	1001:1020	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	5	43	dep	In	848:849	arg1	vivo					851:854	vivo	851:854	vivo	851:854	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	3	44	theme	Si	561:562	arg1	ions					564:567	Si ions	561:567	Si ions in approximately linear manners for around three weeks	561:622	They were able to sustainably release Si ions in approximately linear manners for around three weeks.					
35450620	5	45	from	potency	1098:1104	arg1	use					1109:1111	use	1109:1111	use for wound healing	1109:1129	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	5	46	theme	10 mm	877:881	arg1	defect					909:914	the 10 mm mouse full-thickness skin defect	873:914	the 10 mm mouse full-thickness skin defect	873:914	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
35450620	3	47	from	ions	564:567	arg1	manners					593:599	approximately linear manners	572:599	approximately linear manners	572:599	They were able to sustainably release Si ions in approximately linear manners for around three weeks.					
35450620	4	48	theme	In	625:626	arg1	culture					639:645	In vitro cell culture	625:645	In vitro cell culture	625:645	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	1	49	theme	dual-network	319:330	arg1	structure					332:340	dual-network structure	319:340	dual-network structure	319:340	Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure.					
35450620	4	50	theme	fibroblasts	792:802	arg1	migration					774:782	the migration	770:782	the migration of both fibroblasts and human umbilical vein endothelial cells	770:845	In vitro cell culture revealed that they supported the in-growth and proliferation of seeded cells, and showed the ability to markedly stimulate the migration of both fibroblasts and human umbilical vein endothelial cells.					
35450620	5	51	theme	mouse	883:887	arg1	defect					909:914	the 10 mm mouse full-thickness skin defect	873:914	the 10 mm mouse full-thickness skin defect	873:914	In vivo results based on the 10 mm mouse full-thickness skin defect demonstrated that they were able to fully restore the skin defects with formation of vascularized tissues and complete appendages during two weeks, suggesting their highly promising potency in use for wound healing.					
36921821	6	0	from	60 °C	869:873	arg1	3.08					839:842	3.08	839:842	3.08	839:842	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	6	1	from	40	857:858	arg1	3.08					839:842	3.08	839:842	3.08	839:842	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	1	2	theme	imine-glutaraldehyde	165:184	arg1	immobilizer					198:208	Amidated pectin-polyethylene imine-glutaraldehyde (AP-PEI-GA) immobilizer	136:208	Amidated pectin-polyethylene imine-glutaraldehyde (AP-PEI-GA) immobilizer	136:208	Amidated pectin-polyethylene imine-glutaraldehyde (AP-PEI-GA) immobilizer was prepared.					
36921821	8	3	theme	successive	1080:1089	arg1	cycles					1091:1096	20 successive cycles	1077:1096	20 successive cycles	1077:1096	Im-xylanase showed 100 % activity for 20 successive cycles and hydrolyzed different agro-industrial wastes into reducing sugar and xylooligosaccharides (XOS) with more efficiency on pea peel (PP).					
36921821	8	4	with	xylooligosaccharides	1170:1189	arg1	efficiency					1207:1216	more efficiency	1202:1216	more efficiency	1202:1216	Im-xylanase showed 100 % activity for 20 successive cycles and hydrolyzed different agro-industrial wastes into reducing sugar and xylooligosaccharides (XOS) with more efficiency on pea peel (PP).					
36921821	10	5	theme	anti-oxidant	1454:1465	arg1	activities					1467:1476	prebiotic and anti-oxidant activities	1440:1476	prebiotic and anti-oxidant activities	1440:1476	Extracted XOS showed prebiotic and anti-oxidant activities.					
36921821	3	6	theme	obtained	472:479	arg1	efficient					507:515	efficient	507:515	efficient	507:515	The obtained AP-PEI-GA immobilizer was efficient, and it acquired 3.03 U.g-1 of immobilized xylanase (im-xylanase) activity.					
36921821	3	6	theme	obtained	472:479	arg1	immobilizer					491:501	The obtained AP-PEI-GA immobilizer	468:501	The obtained AP-PEI-GA immobilizer	468:501	The obtained AP-PEI-GA immobilizer was efficient, and it acquired 3.03 U.g-1 of immobilized xylanase (im-xylanase) activity.					
36921821	2	7	dep	processing	365:374	arg1	beads					383:387	the AP beads	376:387	processing the AP beads	365:387	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	7	8	from	40	931:932	arg1	ΔGd					898:900	ΔGd	898:900	ΔGd	898:900	ΔHd and ΔGd for AP-PEI-GA im-xylanase at 40, 50, and 60 °C were higher than those for free form emphasizing more resistance to thermal denaturation.					
36921821	7	8	from	40	931:932	arg1	ΔHd					890:892	ΔHd	890:892	ΔHd	890:892	ΔHd and ΔGd for AP-PEI-GA im-xylanase at 40, 50, and 60 °C were higher than those for free form emphasizing more resistance to thermal denaturation.					
36921821	2	9	dep	%	447:447	arg1	v/v					450:452	v/v	450:452	v/v	450:452	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	8	10	theme	100 	1058:1061	arg1	%					1062:1062	%	1062:1062	%	1062:1062	Im-xylanase showed 100 % activity for 20 successive cycles and hydrolyzed different agro-industrial wastes into reducing sugar and xylooligosaccharides (XOS) with more efficiency on pea peel (PP).					
36921821	0	11	theme	stability	101:109	arg1	studies					111:117	storage stability studies	93:117	storage stability studies	93:117	Xylanase covalent binding onto amidated pectin beads: Optimization, thermal, operational and storage stability studies and application.					
36921821	8	12	with	sugar	1160:1164	arg1	efficiency					1207:1216	more efficiency	1202:1216	more efficiency	1202:1216	Im-xylanase showed 100 % activity for 20 successive cycles and hydrolyzed different agro-industrial wastes into reducing sugar and xylooligosaccharides (XOS) with more efficiency on pea peel (PP).					
36921821	6	13	dep	Aspergillus	745:755	arg1	niger					757:761	niger	757:761	niger	757:761	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	2	14	theme	%	398:398	arg1	solution					410:417	3.4 % (w/w) PEI solution	394:417	3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution	394:465	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	0	15	theme	storage	93:99	arg1	studies					111:117	storage stability studies	93:117	storage stability studies	93:117	Xylanase covalent binding onto amidated pectin beads: Optimization, thermal, operational and storage stability studies and application.					
36921821	9	16	theme	AP-PEI-GA	1236:1244	arg1	im-xylanase					1246:1256	AP-PEI-GA im-xylanase	1236:1256	AP-PEI-GA im-xylanase	1236:1256	AP-PEI-GA im-xylanase, PP weight, and hydrolysis time that should be adopted to obtain the highest reducing sugar and XOS yield were optimized through central composite design (CCD).					
36921821	7	17	theme	thermal	1017:1023	arg1	denaturation					1025:1036	thermal denaturation	1017:1036	thermal denaturation	1017:1036	ΔHd and ΔGd for AP-PEI-GA im-xylanase at 40, 50, and 60 °C were higher than those for free form emphasizing more resistance to thermal denaturation.					
36921821	6	18	theme	thermodynamic	772:784	arg1	T1/2					797:800	T1/2	797:800	T1/2	797:800	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	6	18	theme	thermodynamic	772:784	arg1	properties					786:795	Aspergillus niger xylanase thermodynamic properties T1/2 and D-values	745:813	Aspergillus niger xylanase thermodynamic properties T1/2 and D-values	745:813	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	6	18	theme	thermodynamic	772:784	arg1	D-values					806:813	D-values	806:813	D-values	806:813	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	0	19	theme	Xylanase	0:7	arg1	binding					18:24	Xylanase covalent binding	0:24	Xylanase covalent binding onto amidated pectin beads: Optimization, thermal, operational and storage stability studies and application.	0:134	Xylanase covalent binding onto amidated pectin beads: Optimization, thermal, operational and storage stability studies and application.					
36921821	2	20	theme	3.4 	394:397	arg1	%					398:398	%	398:398	%	398:398	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	0	21	theme	covalent	9:16	arg1	binding					18:24	Xylanase covalent binding	0:24	Xylanase covalent binding onto amidated pectin beads: Optimization, thermal, operational and storage stability studies and application.	0:134	Xylanase covalent binding onto amidated pectin beads: Optimization, thermal, operational and storage stability studies and application.					
36921821	7	22	from	50	935:936	arg1	ΔGd					898:900	ΔGd	898:900	ΔGd	898:900	ΔHd and ΔGd for AP-PEI-GA im-xylanase at 40, 50, and 60 °C were higher than those for free form emphasizing more resistance to thermal denaturation.					
36921821	7	22	from	50	935:936	arg1	ΔHd					890:892	ΔHd	890:892	ΔHd	890:892	ΔHd and ΔGd for AP-PEI-GA im-xylanase at 40, 50, and 60 °C were higher than those for free form emphasizing more resistance to thermal denaturation.					
36921821	7	23	theme	more	998:1001	arg1	resistance					1003:1012	more resistance	998:1012	more resistance to thermal denaturation	998:1036	ΔHd and ΔGd for AP-PEI-GA im-xylanase at 40, 50, and 60 °C were higher than those for free form emphasizing more resistance to thermal denaturation.					
36921821	6	24	theme	xylanase	763:770	arg1	T1/2					797:800	T1/2	797:800	T1/2	797:800	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	6	24	theme	xylanase	763:770	arg1	properties					786:795	Aspergillus niger xylanase thermodynamic properties T1/2 and D-values	745:813	Aspergillus niger xylanase thermodynamic properties T1/2 and D-values	745:813	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	6	24	theme	xylanase	763:770	arg1	D-values					806:813	D-values	806:813	D-values	806:813	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	4	25	theme	AP-PEI-GA	629:637	arg1	im-xylanase					639:649	AP-PEI-GA im-xylanase	629:649	AP-PEI-GA im-xylanase	629:649	The computed Km and Vmax values for AP-PEI-GA im-xylanase were 16.67 mg.ml-1 and 20 g.ml-1.					
36921821	2	26	theme	pH 9.65	422:428	arg1	solution					410:417	3.4 % (w/w) PEI solution	394:417	3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution	394:465	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	10	27	theme	prebiotic	1440:1448	arg1	activities					1467:1476	prebiotic and anti-oxidant activities	1440:1476	prebiotic and anti-oxidant activities	1440:1476	Extracted XOS showed prebiotic and anti-oxidant activities.					
36921821	9	28	theme	PP	1259:1260	arg1	weight					1262:1267	PP weight	1259:1267	PP weight	1259:1267	AP-PEI-GA im-xylanase, PP weight, and hydrolysis time that should be adopted to obtain the highest reducing sugar and XOS yield were optimized through central composite design (CCD).					
36921821	6	29	theme	Aspergillus	745:755	arg1	T1/2					797:800	T1/2	797:800	T1/2	797:800	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	6	29	theme	Aspergillus	745:755	arg1	properties					786:795	Aspergillus niger xylanase thermodynamic properties T1/2 and D-values	745:813	Aspergillus niger xylanase thermodynamic properties T1/2 and D-values	745:813	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	6	29	theme	Aspergillus	745:755	arg1	D-values					806:813	D-values	806:813	D-values	806:813	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	6	30	dep	properties	786:795	arg1	T1/2					797:800	T1/2	797:800	T1/2	797:800	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	6	30	dep	properties	786:795	arg1	properties					786:795	Aspergillus niger xylanase thermodynamic properties T1/2 and D-values	745:813	Aspergillus niger xylanase thermodynamic properties T1/2 and D-values	745:813	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	6	30	dep	properties	786:795	arg1	D-values					806:813	D-values	806:813	D-values	806:813	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	2	31	theme	PEI	406:408	arg1	solution					410:417	3.4 % (w/w) PEI solution	394:417	3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution	394:465	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	8	32	theme	agro-industrial	1123:1137	arg1	wastes					1139:1144	different agro-industrial wastes	1113:1144	different agro-industrial wastes	1113:1144	Im-xylanase showed 100 % activity for 20 successive cycles and hydrolyzed different agro-industrial wastes into reducing sugar and xylooligosaccharides (XOS) with more efficiency on pea peel (PP).					
36921821	2	33	theme	Box-Behnken	322:332	arg1	design					334:339	Box-Behnken design	322:339	Box-Behnken design (BBD)	322:345	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	2	33	theme	Box-Behnken	322:332	arg1	BBD					342:344	BBD	342:344	BBD	342:344	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	1	34	theme	Amidated	136:143	arg1	AP-PEI-GA					187:195	AP-PEI-GA	187:195	AP-PEI-GA	187:195	Amidated pectin-polyethylene imine-glutaraldehyde (AP-PEI-GA) immobilizer was prepared.					
36921821	1	34	theme	Amidated	136:143	arg1	imine-glutaraldehyde					165:184	Amidated pectin-polyethylene imine-glutaraldehyde	136:184	Amidated pectin-polyethylene imine-glutaraldehyde (AP-PEI-GA) immobilizer	136:208	Amidated pectin-polyethylene imine-glutaraldehyde (AP-PEI-GA) immobilizer was prepared.					
36921821	0	35	theme	pectin	40:45	arg1	beads					47:51	amidated pectin beads	31:51	amidated pectin beads	31:51	Xylanase covalent binding onto amidated pectin beads: Optimization, thermal, operational and storage stability studies and application.					
36921821	3	36	theme	xylanase	560:567	arg1	activity					583:590	immobilized xylanase (im-xylanase) activity	548:590	immobilized xylanase (im-xylanase) activity	548:590	The obtained AP-PEI-GA immobilizer was efficient, and it acquired 3.03 U.g-1 of immobilized xylanase (im-xylanase) activity.					
36921821	2	37	theme	%	447:447	arg1	solution					458:465	5.96 % (v/v) GA solution	442:465	5.96 % (v/v) GA solution	442:465	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	0	38	theme	amidated	31:38	arg1	beads					47:51	amidated pectin beads	31:51	amidated pectin beads	31:51	Xylanase covalent binding onto amidated pectin beads: Optimization, thermal, operational and storage stability studies and application.					
36921821	9	39	theme	central	1387:1393	arg1	CCD					1413:1415	CCD	1413:1415	CCD	1413:1415	AP-PEI-GA im-xylanase, PP weight, and hydrolysis time that should be adopted to obtain the highest reducing sugar and XOS yield were optimized through central composite design (CCD).					
36921821	9	39	theme	central	1387:1393	arg1	design					1405:1410	central composite design	1387:1410	central composite design (CCD)	1387:1416	AP-PEI-GA im-xylanase, PP weight, and hydrolysis time that should be adopted to obtain the highest reducing sugar and XOS yield were optimized through central composite design (CCD).					
36921821	4	40	theme	Km	606:607	arg1	16.67 mg.ml-1					656:668	16.67 mg.ml-1	656:668	16.67 mg.ml-1	656:668	The computed Km and Vmax values for AP-PEI-GA im-xylanase were 16.67 mg.ml-1 and 20 g.ml-1.					
36921821	4	40	theme	Km	606:607	arg1	values					618:623	The computed Km and Vmax values	593:623	The computed Km and Vmax values for AP-PEI-GA im-xylanase	593:649	The computed Km and Vmax values for AP-PEI-GA im-xylanase were 16.67 mg.ml-1 and 20 g.ml-1.					
36921821	2	41	theme	5.96 	442:446	arg1	%					447:447	%	447:447	%	447:447	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	9	42	theme	hydrolysis	1274:1283	arg1	time					1285:1288	hydrolysis time	1274:1288	hydrolysis time	1274:1288	AP-PEI-GA im-xylanase, PP weight, and hydrolysis time that should be adopted to obtain the highest reducing sugar and XOS yield were optimized through central composite design (CCD).					
36921821	6	43	from	50	861:862	arg1	3.08					839:842	3.08	839:842	3.08	839:842	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	9	44	theme	composite	1395:1403	arg1	CCD					1413:1415	CCD	1413:1415	CCD	1413:1415	AP-PEI-GA im-xylanase, PP weight, and hydrolysis time that should be adopted to obtain the highest reducing sugar and XOS yield were optimized through central composite design (CCD).					
36921821	9	44	theme	composite	1395:1403	arg1	design					1405:1410	central composite design	1387:1410	central composite design (CCD)	1387:1416	AP-PEI-GA im-xylanase, PP weight, and hydrolysis time that should be adopted to obtain the highest reducing sugar and XOS yield were optimized through central composite design (CCD).					
36921821	7	45	theme	free	976:979	arg1	form					981:984	free form	976:984	free form emphasizing more resistance to thermal denaturation	976:1036	ΔHd and ΔGd for AP-PEI-GA im-xylanase at 40, 50, and 60 °C were higher than those for free form emphasizing more resistance to thermal denaturation.					
36921821	10	46	theme	Extracted	1419:1427	arg1	XOS					1429:1431	Extracted XOS	1419:1431	Extracted XOS	1419:1431	Extracted XOS showed prebiotic and anti-oxidant activities.					
36921821	2	47	theme	ideal	228:232	arg1	protocol					234:241	The ideal protocol	224:241	The ideal protocol that should be adopted during the immobilizer preparation	224:299	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	3	48	theme	immobilized	548:558	arg1	activity					583:590	immobilized xylanase (im-xylanase) activity	548:590	immobilized xylanase (im-xylanase) activity	548:590	The obtained AP-PEI-GA immobilizer was efficient, and it acquired 3.03 U.g-1 of immobilized xylanase (im-xylanase) activity.					
36921821	8	49	theme	pea	1221:1223	arg1	PP					1231:1232	PP	1231:1232	PP	1231:1232	Im-xylanase showed 100 % activity for 20 successive cycles and hydrolyzed different agro-industrial wastes into reducing sugar and xylooligosaccharides (XOS) with more efficiency on pea peel (PP).					
36921821	8	49	theme	pea	1221:1223	arg1	peel					1225:1228	pea peel	1221:1228	pea peel (PP)	1221:1233	Im-xylanase showed 100 % activity for 20 successive cycles and hydrolyzed different agro-industrial wastes into reducing sugar and xylooligosaccharides (XOS) with more efficiency on pea peel (PP).					
36921821	8	50	theme	different	1113:1121	arg1	wastes					1139:1144	different agro-industrial wastes	1113:1144	different agro-industrial wastes	1113:1144	Im-xylanase showed 100 % activity for 20 successive cycles and hydrolyzed different agro-industrial wastes into reducing sugar and xylooligosaccharides (XOS) with more efficiency on pea peel (PP).					
36921821	3	51	theme	activity	583:590	arg1	3.03 U.g-1					534:543	3.03 U.g-1	534:543	3.03 U.g-1 of immobilized xylanase (im-xylanase) activity	534:590	The obtained AP-PEI-GA immobilizer was efficient, and it acquired 3.03 U.g-1 of immobilized xylanase (im-xylanase) activity.					
36921821	2	52	theme	immobilizer	277:287	arg1	preparation					289:299	the immobilizer preparation	273:299	the immobilizer preparation	273:299	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	7	53	theme	AP-PEI-GA	906:914	arg1	im-xylanase					916:926	AP-PEI-GA im-xylanase	906:926	AP-PEI-GA im-xylanase	906:926	ΔHd and ΔGd for AP-PEI-GA im-xylanase at 40, 50, and 60 °C were higher than those for free form emphasizing more resistance to thermal denaturation.					
36921821	0	54	dep	binding	18:24	arg1	application					123:133	application	123:133	application	123:133	Xylanase covalent binding onto amidated pectin beads: Optimization, thermal, operational and storage stability studies and application.					
36921821	0	54	dep	binding	18:24	arg1	thermal					68:74	thermal	68:74	thermal	68:74	Xylanase covalent binding onto amidated pectin beads: Optimization, thermal, operational and storage stability studies and application.					
36921821	0	54	dep	binding	18:24	arg1	Optimization					54:65	Optimization	54:65	Optimization	54:65	Xylanase covalent binding onto amidated pectin beads: Optimization, thermal, operational and storage stability studies and application.					
36921821	6	55	theme	covalent	713:720	arg1	coupling					722:729	covalent coupling	713:729	covalent coupling to AP-PEI-GA	713:742	Through covalent coupling to AP-PEI-GA, Aspergillus niger xylanase thermodynamic properties T1/2 and D-values were increased by 2.05, 3.08, and 1.35 at 40, 50, and 60 °C, respectively.					
36921821	3	56	theme	AP-PEI-GA	481:489	arg1	efficient					507:515	efficient	507:515	efficient	507:515	The obtained AP-PEI-GA immobilizer was efficient, and it acquired 3.03 U.g-1 of immobilized xylanase (im-xylanase) activity.					
36921821	3	56	theme	AP-PEI-GA	481:489	arg1	immobilizer					491:501	The obtained AP-PEI-GA immobilizer	468:501	The obtained AP-PEI-GA immobilizer	468:501	The obtained AP-PEI-GA immobilizer was efficient, and it acquired 3.03 U.g-1 of immobilized xylanase (im-xylanase) activity.					
36921821	8	57	theme	%	1062:1062	arg1	activity					1064:1071	100 % activity	1058:1071	100 % activity	1058:1071	Im-xylanase showed 100 % activity for 20 successive cycles and hydrolyzed different agro-industrial wastes into reducing sugar and xylooligosaccharides (XOS) with more efficiency on pea peel (PP).					
36921821	7	58	from	60 °C	943:947	arg1	ΔGd					898:900	ΔGd	898:900	ΔGd	898:900	ΔHd and ΔGd for AP-PEI-GA im-xylanase at 40, 50, and 60 °C were higher than those for free form emphasizing more resistance to thermal denaturation.					
36921821	7	58	from	60 °C	943:947	arg1	ΔHd					890:892	ΔHd	890:892	ΔHd	890:892	ΔHd and ΔGd for AP-PEI-GA im-xylanase at 40, 50, and 60 °C were higher than those for free form emphasizing more resistance to thermal denaturation.					
36921821	2	59	dep	%	398:398	arg1	w/w					401:403	w/w	401:403	w/w	401:403	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	4	60	theme	computed	597:604	arg1	16.67 mg.ml-1					656:668	16.67 mg.ml-1	656:668	16.67 mg.ml-1	656:668	The computed Km and Vmax values for AP-PEI-GA im-xylanase were 16.67 mg.ml-1 and 20 g.ml-1.					
36921821	4	60	theme	computed	597:604	arg1	values					618:623	The computed Km and Vmax values	593:623	The computed Km and Vmax values for AP-PEI-GA im-xylanase	593:649	The computed Km and Vmax values for AP-PEI-GA im-xylanase were 16.67 mg.ml-1 and 20 g.ml-1.					
36921821	2	61	theme	AP	380:381	arg1	beads					383:387	the AP beads	376:387	processing the AP beads	365:387	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	4	62	theme	Vmax	613:616	arg1	16.67 mg.ml-1					656:668	16.67 mg.ml-1	656:668	16.67 mg.ml-1	656:668	The computed Km and Vmax values for AP-PEI-GA im-xylanase were 16.67 mg.ml-1 and 20 g.ml-1.					
36921821	4	62	theme	Vmax	613:616	arg1	values					618:623	The computed Km and Vmax values	593:623	The computed Km and Vmax values for AP-PEI-GA im-xylanase	593:649	The computed Km and Vmax values for AP-PEI-GA im-xylanase were 16.67 mg.ml-1 and 20 g.ml-1.					
36921821	2	63	theme	GA	455:456	arg1	solution					458:465	5.96 % (v/v) GA solution	442:465	5.96 % (v/v) GA solution	442:465	The ideal protocol that should be adopted during the immobilizer preparation was investigated via Box-Behnken design (BBD), and it comprised processing the AP beads with 3.4 % (w/w) PEI solution of pH 9.65 followed by 5.96 % (v/v) GA solution.					
36921821	9	64	theme	XOS	1354:1356	arg1	yield					1358:1362	XOS yield	1354:1362	XOS yield	1354:1362	AP-PEI-GA im-xylanase, PP weight, and hydrolysis time that should be adopted to obtain the highest reducing sugar and XOS yield were optimized through central composite design (CCD).					
36921821	1	65	theme	pectin-polyethylene	145:163	arg1	AP-PEI-GA					187:195	AP-PEI-GA	187:195	AP-PEI-GA	187:195	Amidated pectin-polyethylene imine-glutaraldehyde (AP-PEI-GA) immobilizer was prepared.					
36921821	1	65	theme	pectin-polyethylene	145:163	arg1	imine-glutaraldehyde					165:184	Amidated pectin-polyethylene imine-glutaraldehyde	136:184	Amidated pectin-polyethylene imine-glutaraldehyde (AP-PEI-GA) immobilizer	136:208	Amidated pectin-polyethylene imine-glutaraldehyde (AP-PEI-GA) immobilizer was prepared.					
35525868	7	0	theme	antifungal	1163:1172	arg1	activity					1174:1181	enhanced antifungal activity	1154:1181	enhanced antifungal activity against Candida albicans	1154:1206	The nanoparticle-loaded gel containing CMCh demonstrated enhanced antifungal activity against Candida albicans.					
35525868	8	1	theme	containing	1229:1238	arg1	CMCh					1240:1243	The optimized batch containing CMCh	1209:1243	The optimized batch containing CMCh	1209:1243	The optimized batch containing CMCh showed improved mucoadhesion by 2.86-fold compared to VCZ nanosuspension.					
35525868	11	2	theme	nanoparticulate	1661:1675	arg1	gel					1696:1698	nanoparticulate in situ ophthalmic gel	1661:1698	nanoparticulate in situ ophthalmic gel	1661:1698	The present study concludes that the VCZ loaded nanoparticulate in situ ophthalmic gel using CMCh may act as a potential alternative for traditional eye drops.					
35525868	1	3	theme	ocular	322:327	arg1	diseases					329:336	ocular diseases	322:336	ocular diseases	322:336	The research study reflects the development of novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases.					
35525868	9	4	theme	goat	1424:1427	arg1	cornea					1429:1434	goat cornea	1424:1434	goat cornea estimated via fluorescent microscope	1424:1471	The drug release was prolonged up to 8 h with an ex vivo study suggesting the enhanced permeation across goat cornea estimated via fluorescent microscope.					
35525868	9	5	dep	ex	1368:1369	arg1	vivo					1371:1374	vivo	1371:1374	vivo	1371:1374	The drug release was prolonged up to 8 h with an ex vivo study suggesting the enhanced permeation across goat cornea estimated via fluorescent microscope.					
35525868	0	6	dep	In	134:135	arg1	vitro					137:141	vitro	137:141	vitro	137:141	Statistical optimization of voriconazole nanoparticles loaded carboxymethyl chitosan-poloxamer based in situ gel for ocular delivery: In vitro, ex vivo, and toxicity assessment.					
35525868	0	7	theme	ocular	117:122	arg1	delivery					124:131	ocular delivery	117:131	ocular delivery	117:131	Statistical optimization of voriconazole nanoparticles loaded carboxymethyl chitosan-poloxamer based in situ gel for ocular delivery: In vitro, ex vivo, and toxicity assessment.					
35525868	1	8	theme	diseases	329:336	arg1	management					308:317	the management	304:317	the management of ocular diseases	304:336	The research study reflects the development of novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases.					
35525868	4	9	theme	drug	817:820	arg1	release					822:828	drug release	817:828	drug release	817:828	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	1	10	theme	loaded	250:255	arg1	NPs					272:274	NPs	272:274	NPs	272:274	The research study reflects the development of novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases.					
35525868	1	10	theme	loaded	250:255	arg1	nanoparticles					257:269	novel voriconazole (VCZ) loaded nanoparticles	225:269	novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases	225:336	The research study reflects the development of novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases.					
35525868	5	11	theme	functional	882:891	arg1	groups					893:898	carboxyl surface functional groups	865:898	carboxyl surface functional groups	865:898	The etherification process grafts carboxyl surface functional groups, on chitosan, and was confirmed by FTIR and NMR studies.					
35525868	6	12	with	clear	1023:1027	arg1	temperature					1059:1069	gelation temperature	1050:1069	gelation temperature varying from 33 to 40 °C	1050:1094	The developed CMCh-poloxamer based gelling system was found to be clear and transparent with gelation temperature varying from 33 to 40 °C.					
35525868	8	13	theme	improved	1252:1259	arg1	mucoadhesion					1261:1272	improved mucoadhesion	1252:1272	improved mucoadhesion by 2.86-fold	1252:1285	The optimized batch containing CMCh showed improved mucoadhesion by 2.86-fold compared to VCZ nanosuspension.					
35525868	1	14	theme	nanoparticles	257:269	arg1	development					210:220	the development	206:220	the development of novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases	206:336	The research study reflects the development of novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases.					
35525868	7	15	theme	containing	1125:1134	arg1	CMCh					1136:1139	The nanoparticle-loaded gel containing CMCh	1097:1139	The nanoparticle-loaded gel containing CMCh	1097:1139	The nanoparticle-loaded gel containing CMCh demonstrated enhanced antifungal activity against Candida albicans.					
35525868	4	16	dep	index	726:730	arg1	PDI					733:735	PDI	733:735	PDI	733:735	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	8	17	theme	batch	1223:1227	arg1	CMCh					1240:1243	The optimized batch containing CMCh	1209:1243	The optimized batch containing CMCh	1209:1243	The optimized batch containing CMCh showed improved mucoadhesion by 2.86-fold compared to VCZ nanosuspension.					
35525868	4	18	theme	0.1	738:740	arg1	PDI					733:735	PDI	733:735	PDI	733:735	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	8	19	theme	optimized	1213:1221	arg1	CMCh					1240:1243	The optimized batch containing CMCh	1209:1243	The optimized batch containing CMCh	1209:1243	The optimized batch containing CMCh showed improved mucoadhesion by 2.86-fold compared to VCZ nanosuspension.					
35525868	4	20	theme	encapsulation	639:651	arg1	efficiency					653:662	the encapsulation efficiency	635:662	the encapsulation efficiency (89.6 ± 1.2%)	635:676	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	4	20	theme	encapsulation	639:651	arg1	%					675:675	89.6 ± 1.2%	665:675	89.6 ± 1.2%	665:675	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	11	21	theme	eye	1762:1764	arg1	drops					1766:1770	traditional eye drops	1750:1770	traditional eye drops	1750:1770	The present study concludes that the VCZ loaded nanoparticulate in situ ophthalmic gel using CMCh may act as a potential alternative for traditional eye drops.					
35525868	5	22	theme	etherification	835:848	arg1	process					850:856	The etherification process	831:856	The etherification process	831:856	The etherification process grafts carboxyl surface functional groups, on chitosan, and was confirmed by FTIR and NMR studies.					
35525868	4	23	theme	saturation	779:788	arg1	solubility					790:799	saturation solubility	779:799	saturation solubility	779:799	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	2	24	dep	in	343:344	arg1	situ					346:349	situ	346:349	situ	346:349	The in situ ophthalmic gel was prepared by incorporating NPs into carboxymethyl chitosan (CMCh) and poloxamer.					
35525868	7	25	theme	nanoparticle-loaded	1101:1119	arg1	CMCh					1136:1139	The nanoparticle-loaded gel containing CMCh	1097:1139	The nanoparticle-loaded gel containing CMCh	1097:1139	The nanoparticle-loaded gel containing CMCh demonstrated enhanced antifungal activity against Candida albicans.					
35525868	4	26	theme	DSC	802:804	arg1	study					806:810	DSC study	802:810	DSC study	802:810	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	11	27	theme	present	1617:1623	arg1	study					1625:1629	The present study	1613:1629	The present study	1613:1629	The present study concludes that the VCZ loaded nanoparticulate in situ ophthalmic gel using CMCh may act as a potential alternative for traditional eye drops.					
35525868	3	28	theme	nanoparticles	535:547	arg1	preparation					520:530	the preparation	516:530	the preparation of nanoparticles	516:547	The central composite design was used to optimize the process for the preparation of nanoparticles by the o/w solvent evaporation method.					
35525868	11	29	theme	in	1677:1678	arg1	gel					1696:1698	nanoparticulate in situ ophthalmic gel	1661:1698	nanoparticulate in situ ophthalmic gel	1661:1698	The present study concludes that the VCZ loaded nanoparticulate in situ ophthalmic gel using CMCh may act as a potential alternative for traditional eye drops.					
35525868	6	30	dep	40 °C	1090:1094	arg1	to					1087:1088	to	1087:1088	to	1087:1088	The developed CMCh-poloxamer based gelling system was found to be clear and transparent with gelation temperature varying from 33 to 40 °C.					
35525868	0	31	theme	Statistical	0:10	arg1	optimization					12:23	Statistical optimization	0:23	Statistical optimization of voriconazole	0:39	Statistical optimization of voriconazole nanoparticles loaded carboxymethyl chitosan-poloxamer based in situ gel for ocular delivery: In vitro, ex vivo, and toxicity assessment.					
35525868	2	32	theme	ophthalmic	351:360	arg1	gel					362:364	The in situ ophthalmic gel	339:364	The in situ ophthalmic gel	339:364	The in situ ophthalmic gel was prepared by incorporating NPs into carboxymethyl chitosan (CMCh) and poloxamer.					
35525868	0	33	theme	voriconazole	28:39	arg1	optimization					12:23	Statistical optimization	0:23	Statistical optimization of voriconazole	0:39	Statistical optimization of voriconazole nanoparticles loaded carboxymethyl chitosan-poloxamer based in situ gel for ocular delivery: In vitro, ex vivo, and toxicity assessment.					
35525868	10	34	theme	chorioallantoic	1587:1601	arg1	membrane					1603:1610	the chorioallantoic membrane	1583:1610	the chorioallantoic membrane	1583:1610	The hen's egg chorioallantoic membrane study revealed that the formulation was non-irritant and tolerated by the chorioallantoic membrane.					
35525868	5	35	theme	surface	874:880	arg1	groups					893:898	carboxyl surface functional groups	865:898	carboxyl surface functional groups	865:898	The etherification process grafts carboxyl surface functional groups, on chitosan, and was confirmed by FTIR and NMR studies.					
35525868	0	36	dep	in	101:102	arg1	situ					104:107	situ	104:107	situ	104:107	Statistical optimization of voriconazole nanoparticles loaded carboxymethyl chitosan-poloxamer based in situ gel for ocular delivery: In vitro, ex vivo, and toxicity assessment.					
35525868	5	37	theme	NMR	944:946	arg1	studies					948:954	FTIR and NMR studies	935:954	studies	948:954	The etherification process grafts carboxyl surface functional groups, on chitosan, and was confirmed by FTIR and NMR studies.					
35525868	6	38	theme	gelling	992:998	arg1	clear					1023:1027	clear	1023:1027	clear	1023:1027	The developed CMCh-poloxamer based gelling system was found to be clear and transparent with gelation temperature varying from 33 to 40 °C.					
35525868	6	38	theme	gelling	992:998	arg1	system					1000:1005	The developed CMCh-poloxamer based gelling system	957:1005	The developed CMCh-poloxamer based gelling system	957:1005	The developed CMCh-poloxamer based gelling system was found to be clear and transparent with gelation temperature varying from 33 to 40 °C.					
35525868	2	39	theme	carboxymethyl	405:417	arg1	CMCh					429:432	CMCh	429:432	CMCh	429:432	The in situ ophthalmic gel was prepared by incorporating NPs into carboxymethyl chitosan (CMCh) and poloxamer.					
35525868	2	39	theme	carboxymethyl	405:417	arg1	chitosan					419:426	carboxymethyl chitosan	405:426	carboxymethyl chitosan (CMCh)	405:433	The in situ ophthalmic gel was prepared by incorporating NPs into carboxymethyl chitosan (CMCh) and poloxamer.					
35525868	9	40	theme	drug	1323:1326	arg1	release					1328:1334	The drug release	1319:1334	The drug release	1319:1334	The drug release was prolonged up to 8 h with an ex vivo study suggesting the enhanced permeation across goat cornea estimated via fluorescent microscope.					
35525868	11	41	theme	traditional	1750:1760	arg1	drops					1766:1770	traditional eye drops	1750:1770	traditional eye drops	1750:1770	The present study concludes that the VCZ loaded nanoparticulate in situ ophthalmic gel using CMCh may act as a potential alternative for traditional eye drops.					
35525868	4	42	theme	polydispersity	711:724	arg1	index					726:730	polydispersity index	711:730	polydispersity index (PDI, 0.1)	711:741	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	6	43	theme	based	986:990	arg1	clear					1023:1027	clear	1023:1027	clear	1023:1027	The developed CMCh-poloxamer based gelling system was found to be clear and transparent with gelation temperature varying from 33 to 40 °C.					
35525868	6	43	theme	based	986:990	arg1	system					1000:1005	The developed CMCh-poloxamer based gelling system	957:1005	The developed CMCh-poloxamer based gelling system	957:1005	The developed CMCh-poloxamer based gelling system was found to be clear and transparent with gelation temperature varying from 33 to 40 °C.					
35525868	0	44	theme	loaded	55:60	arg1	chitosan-poloxamer					76:93	loaded carboxymethyl chitosan-poloxamer	55:93	loaded carboxymethyl chitosan-poloxamer based in situ gel for ocular delivery: In vitro, ex vivo, and toxicity assessment	55:175	Statistical optimization of voriconazole nanoparticles loaded carboxymethyl chitosan-poloxamer based in situ gel for ocular delivery: In vitro, ex vivo, and toxicity assessment.					
35525868	8	45	theme	VCZ	1299:1301	arg1	nanosuspension					1303:1316	VCZ nanosuspension	1299:1316	VCZ nanosuspension	1299:1316	The optimized batch containing CMCh showed improved mucoadhesion by 2.86-fold compared to VCZ nanosuspension.					
35525868	7	46	theme	gel	1121:1123	arg1	CMCh					1136:1139	The nanoparticle-loaded gel containing CMCh	1097:1139	The nanoparticle-loaded gel containing CMCh	1097:1139	The nanoparticle-loaded gel containing CMCh demonstrated enhanced antifungal activity against Candida albicans.					
35525868	3	47	theme	solvent	560:566	arg1	method					580:585	the o/w solvent evaporation method	552:585	the o/w solvent evaporation method	552:585	The central composite design was used to optimize the process for the preparation of nanoparticles by the o/w solvent evaporation method.					
35525868	1	48	theme	research	182:189	arg1	study					191:195	The research study	178:195	The research study	178:195	The research study reflects the development of novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases.					
35525868	6	49	theme	CMCh-poloxamer	971:984	arg1	clear					1023:1027	clear	1023:1027	clear	1023:1027	The developed CMCh-poloxamer based gelling system was found to be clear and transparent with gelation temperature varying from 33 to 40 °C.					
35525868	6	49	theme	CMCh-poloxamer	971:984	arg1	system					1000:1005	The developed CMCh-poloxamer based gelling system	957:1005	The developed CMCh-poloxamer based gelling system	957:1005	The developed CMCh-poloxamer based gelling system was found to be clear and transparent with gelation temperature varying from 33 to 40 °C.					
35525868	9	50	theme	enhanced	1397:1404	arg1	permeation					1406:1415	the enhanced permeation	1393:1415	the enhanced permeation across goat cornea estimated via fluorescent microscope	1393:1471	The drug release was prolonged up to 8 h with an ex vivo study suggesting the enhanced permeation across goat cornea estimated via fluorescent microscope.					
35525868	1	51	theme	prolonged	281:289	arg1	delivery					291:298	prolonged delivery	281:298	prolonged delivery for the management of ocular diseases	281:336	The research study reflects the development of novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases.					
35525868	11	52	dep	in	1677:1678	arg1	situ					1680:1683	situ	1680:1683	situ	1680:1683	The present study concludes that the VCZ loaded nanoparticulate in situ ophthalmic gel using CMCh may act as a potential alternative for traditional eye drops.					
35525868	3	53	theme	evaporation	568:578	arg1	method					580:585	the o/w solvent evaporation method	552:585	the o/w solvent evaporation method	552:585	The central composite design was used to optimize the process for the preparation of nanoparticles by the o/w solvent evaporation method.					
35525868	6	54	theme	developed	961:969	arg1	clear					1023:1027	clear	1023:1027	clear	1023:1027	The developed CMCh-poloxamer based gelling system was found to be clear and transparent with gelation temperature varying from 33 to 40 °C.					
35525868	6	54	theme	developed	961:969	arg1	system					1000:1005	The developed CMCh-poloxamer based gelling system	957:1005	The developed CMCh-poloxamer based gelling system	957:1005	The developed CMCh-poloxamer based gelling system was found to be clear and transparent with gelation temperature varying from 33 to 40 °C.					
35525868	10	55	theme	egg	1484:1486	arg1	study					1513:1517	The hen's egg chorioallantoic membrane study	1474:1517	The hen's egg chorioallantoic membrane study	1474:1517	The hen's egg chorioallantoic membrane study revealed that the formulation was non-irritant and tolerated by the chorioallantoic membrane.					
35525868	10	56	theme	membrane	1504:1511	arg1	study					1513:1517	The hen's egg chorioallantoic membrane study	1474:1517	The hen's egg chorioallantoic membrane study	1474:1517	The hen's egg chorioallantoic membrane study revealed that the formulation was non-irritant and tolerated by the chorioallantoic membrane.					
35525868	11	57	theme	ophthalmic	1685:1694	arg1	gel					1696:1698	nanoparticulate in situ ophthalmic gel	1661:1698	nanoparticulate in situ ophthalmic gel	1661:1698	The present study concludes that the VCZ loaded nanoparticulate in situ ophthalmic gel using CMCh may act as a potential alternative for traditional eye drops.					
35525868	6	58	theme	gelation	1050:1057	arg1	temperature					1059:1069	gelation temperature	1050:1069	gelation temperature varying from 33 to 40 °C	1050:1094	The developed CMCh-poloxamer based gelling system was found to be clear and transparent with gelation temperature varying from 33 to 40 °C.					
35525868	3	59	theme	composite	462:470	arg1	design					472:477	The central composite design	450:477	The central composite design	450:477	The central composite design was used to optimize the process for the preparation of nanoparticles by the o/w solvent evaporation method.					
35525868	0	60	theme	carboxymethyl	62:74	arg1	chitosan-poloxamer					76:93	loaded carboxymethyl chitosan-poloxamer	55:93	loaded carboxymethyl chitosan-poloxamer based in situ gel for ocular delivery: In vitro, ex vivo, and toxicity assessment	55:175	Statistical optimization of voriconazole nanoparticles loaded carboxymethyl chitosan-poloxamer based in situ gel for ocular delivery: In vitro, ex vivo, and toxicity assessment.					
35525868	10	61	theme	chorioallantoic	1488:1502	arg1	study					1513:1517	The hen's egg chorioallantoic membrane study	1474:1517	The hen's egg chorioallantoic membrane study	1474:1517	The hen's egg chorioallantoic membrane study revealed that the formulation was non-irritant and tolerated by the chorioallantoic membrane.					
35525868	3	62	theme	o/w	556:558	arg1	method					580:585	the o/w solvent evaporation method	552:585	the o/w solvent evaporation method	552:585	The central composite design was used to optimize the process for the preparation of nanoparticles by the o/w solvent evaporation method.					
35525868	4	63	theme	particle	679:686	arg1	size					688:691	particle size	679:691	particle size (219.3 ± 1.8 nm)	679:708	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	4	63	theme	particle	679:686	arg1	219.3 ± 1.8 nm					694:707	219.3 ± 1.8 nm	694:707	219.3 ± 1.8 nm	694:707	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	5	64	from	grafts	858:863	arg1	chitosan					904:911	chitosan	904:911	chitosan	904:911	The etherification process grafts carboxyl surface functional groups, on chitosan, and was confirmed by FTIR and NMR studies.					
35525868	0	65	theme	toxicity	157:164	arg1	assessment					166:175	toxicity assessment	157:175	toxicity assessment	157:175	Statistical optimization of voriconazole nanoparticles loaded carboxymethyl chitosan-poloxamer based in situ gel for ocular delivery: In vitro, ex vivo, and toxicity assessment.					
35525868	3	66	used	used	483:486	arg2	design					472:477	The central composite design	450:477	The central composite design	450:477	The central composite design was used to optimize the process for the preparation of nanoparticles by the o/w solvent evaporation method.					
35525868	5	67	theme	carboxyl	865:872	arg1	groups					893:898	carboxyl surface functional groups	865:898	carboxyl surface functional groups	865:898	The etherification process grafts carboxyl surface functional groups, on chitosan, and was confirmed by FTIR and NMR studies.					
35525868	11	68	theme	potential	1724:1732	arg1	alternative					1734:1744	a potential alternative	1722:1744	a potential alternative for traditional eye drops	1722:1770	The present study concludes that the VCZ loaded nanoparticulate in situ ophthalmic gel using CMCh may act as a potential alternative for traditional eye drops.					
35525868	11	68	theme	potential	1724:1732	arg1	VCZ					1650:1652	the VCZ	1646:1652	the VCZ loaded nanoparticulate in situ ophthalmic gel using CMCh	1646:1709	The present study concludes that the VCZ loaded nanoparticulate in situ ophthalmic gel using CMCh may act as a potential alternative for traditional eye drops.					
35525868	3	69	theme	central	454:460	arg1	design					472:477	The central composite design	450:477	The central composite design	450:477	The central composite design was used to optimize the process for the preparation of nanoparticles by the o/w solvent evaporation method.					
35525868	1	70	theme	novel	225:229	arg1	voriconazole					231:242	novel voriconazole	225:242	novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases	225:336	The research study reflects the development of novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases.					
35525868	1	70	theme	novel	225:229	arg1	VCZ					245:247	VCZ	245:247	VCZ	245:247	The research study reflects the development of novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases.					
35525868	0	71	theme	in	101:102	arg1	gel					109:111	in situ gel	101:111	in situ gel	101:111	Statistical optimization of voriconazole nanoparticles loaded carboxymethyl chitosan-poloxamer based in situ gel for ocular delivery: In vitro, ex vivo, and toxicity assessment.					
35525868	9	72	theme	fluorescent	1450:1460	arg1	microscope					1462:1471	fluorescent microscope	1450:1471	fluorescent microscope	1450:1471	The drug release was prolonged up to 8 h with an ex vivo study suggesting the enhanced permeation across goat cornea estimated via fluorescent microscope.					
35525868	5	73	theme	FTIR	935:938	arg1	studies					948:954	FTIR and NMR studies	935:954	studies	948:954	The etherification process grafts carboxyl surface functional groups, on chitosan, and was confirmed by FTIR and NMR studies.					
35525868	4	74	theme	developed	592:600	arg1	nanoparticles					602:614	The developed nanoparticles	588:614	The developed nanoparticles	588:614	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	0	75	dep	ex	144:145	arg1	vivo					147:150	vivo	147:150	vivo	147:150	Statistical optimization of voriconazole nanoparticles loaded carboxymethyl chitosan-poloxamer based in situ gel for ocular delivery: In vitro, ex vivo, and toxicity assessment.					
35525868	1	76	theme	voriconazole	231:242	arg1	NPs					272:274	NPs	272:274	NPs	272:274	The research study reflects the development of novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases.					
35525868	1	76	theme	voriconazole	231:242	arg1	nanoparticles					257:269	novel voriconazole (VCZ) loaded nanoparticles	225:269	novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases	225:336	The research study reflects the development of novel voriconazole (VCZ) loaded nanoparticles (NPs) for prolonged delivery for the management of ocular diseases.					
35525868	7	77	theme	Candida	1191:1197	arg1	albicans					1199:1206	Candida albicans	1191:1206	Candida albicans	1191:1206	The nanoparticle-loaded gel containing CMCh demonstrated enhanced antifungal activity against Candida albicans.					
35525868	4	78	theme	zeta	744:747	arg1	- 21.1 ± 1.12 mV					760:775	- 21.1 ± 1.12 mV	760:775	- 21.1 ± 1.12 mV	760:775	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	4	78	theme	zeta	744:747	arg1	potential					749:757	zeta potential	744:757	zeta potential (- 21.1 ± 1.12 mV)	744:776	The developed nanoparticles were evaluated for the encapsulation efficiency (89.6 ± 1.2%), particle size (219.3 ± 1.8 nm), polydispersity index (PDI, 0.1), zeta potential (- 21.1 ± 1.12 mV), saturation solubility, DSC study, and drug release.					
35525868	9	79	with	8 h	1356:1358	arg1	study					1376:1380	an ex vivo study	1365:1380	an ex vivo study	1365:1380	The drug release was prolonged up to 8 h with an ex vivo study suggesting the enhanced permeation across goat cornea estimated via fluorescent microscope.					
35525868	2	80	theme	in	343:344	arg1	gel					362:364	The in situ ophthalmic gel	339:364	The in situ ophthalmic gel	339:364	The in situ ophthalmic gel was prepared by incorporating NPs into carboxymethyl chitosan (CMCh) and poloxamer.					
35525868	9	81	theme	ex	1368:1369	arg1	study					1376:1380	an ex vivo study	1365:1380	an ex vivo study	1365:1380	The drug release was prolonged up to 8 h with an ex vivo study suggesting the enhanced permeation across goat cornea estimated via fluorescent microscope.					
35525868	7	82	theme	enhanced	1154:1161	arg1	activity					1174:1181	enhanced antifungal activity	1154:1181	enhanced antifungal activity against Candida albicans	1154:1206	The nanoparticle-loaded gel containing CMCh demonstrated enhanced antifungal activity against Candida albicans.					
36745878	4	0	theme	reactions	638:646	arg1	phase					671:675	an external organic phase	651:675	an external organic phase	651:675	Composite alginate hydrogel particles were generated via a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase.					
36745878	4	0	theme	reactions	638:646	arg1	use					622:624	use	622:624	use of chemical reactions	622:646	Composite alginate hydrogel particles were generated via a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase.					
36745878	6	1	theme	concentration	871:883	arg1	formulations					900:911	stable, high concentration and injectable formulations	858:911	stable, high concentration and injectable formulations	858:911	Crystalline forms of the mAb encapsuled in the hydrogel particles led to stable, high concentration and injectable formulations.					
36745878	8	2	with	comparison	1187:1196	arg1	approaches					1215:1224	formulation approaches	1203:1224	formulation approaches involving organic phases and chemical reactions	1203:1272	Bioanalytical studies confirmed superior maintained quality of the antibody in comparison with formulation approaches involving organic phases and chemical reactions.					
36745878	11	3	theme	crystalline	1572:1582	arg1	particles					1617:1625	crystalline antibody-laden alginate hydrogel particles	1572:1625	crystalline antibody-laden alginate hydrogel particles in Wistar rats	1572:1640	Furthermore, the pharmacokinetics of the subcutaneously delivered human ant-PD-1 mAb in crystalline antibody-laden alginate hydrogel particles in Wistar rats was evaluated.					
36745878	11	4	theme	mAb	1565:1567	arg1	pharmacokinetics					1501:1516	the pharmacokinetics	1497:1516	the pharmacokinetics of the subcutaneously delivered human ant-PD-1 mAb in crystalline antibody-laden alginate hydrogel particles in Wistar rats	1497:1640	Furthermore, the pharmacokinetics of the subcutaneously delivered human ant-PD-1 mAb in crystalline antibody-laden alginate hydrogel particles in Wistar rats was evaluated.					
36745878	7	5	theme	Formulation	914:924	arg1	concentrations					926:939	Formulation concentrations	914:939	Formulation concentrations as high as 315 mg/ml antibody	914:969	Formulation concentrations as high as 315 mg/ml antibody were achieved with encapsulation efficiencies in the range of 89-97%, with no perceivable increase in the number of antibody aggregates.					
36745878	7	6	from	increase	1061:1068	arg1	number					1077:1082	the number	1073:1082	the number of antibody aggregates	1073:1105	Formulation concentrations as high as 315 mg/ml antibody were achieved with encapsulation efficiencies in the range of 89-97%, with no perceivable increase in the number of antibody aggregates.					
36745878	5	7	theme	anti-PD-1	704:712	arg1	pembrolizumab					724:736	pembrolizumab	724:736	pembrolizumab	724:736	Crystalline suspension of anti-PD-1 antibody (pembrolizumab) was utilized as a model therapeutic antibody.					
36745878	5	7	theme	anti-PD-1	704:712	arg1	antibody					714:721	anti-PD-1 antibody	704:721	anti-PD-1 antibody (pembrolizumab)	704:737	Crystalline suspension of anti-PD-1 antibody (pembrolizumab) was utilized as a model therapeutic antibody.					
36745878	5	8	used	utilized	743:750	arg2	antibody					775:782	a model therapeutic antibody	755:782	a model therapeutic antibody	755:782	Crystalline suspension of anti-PD-1 antibody (pembrolizumab) was utilized as a model therapeutic antibody.					
36745878	5	8	used	utilized	743:750	arg2	suspension					690:699	Crystalline suspension	678:699	Crystalline suspension of anti-PD-1 antibody (pembrolizumab)	678:737	Crystalline suspension of anti-PD-1 antibody (pembrolizumab) was utilized as a model therapeutic antibody.					
36745878	5	9	theme	therapeutic	763:773	arg1	antibody					775:782	a model therapeutic antibody	755:782	a model therapeutic antibody	755:782	Crystalline suspension of anti-PD-1 antibody (pembrolizumab) was utilized as a model therapeutic antibody.					
36745878	5	9	theme	therapeutic	763:773	arg1	suspension					690:699	Crystalline suspension	678:699	Crystalline suspension of anti-PD-1 antibody (pembrolizumab)	678:737	Crystalline suspension of anti-PD-1 antibody (pembrolizumab) was utilized as a model therapeutic antibody.					
36745878	9	10	theme	oxide	1359:1363	arg1	alginates					1365:1373	partially oxide alginates	1349:1373	partially oxide alginates	1349:1373	We illustrated tuning the alginate particles' disintegration by utilizing partially oxide alginates.					
36745878	2	11	theme	small	238:242	arg1	volumes					254:260	small injection volumes	238:260	small injection volumes	238:260	However, formulating mAbs for small injection volumes at high concentrations with suitable stability and injectability is a significant challenge.					
36745878	10	12	dep	in	1467:1468	arg1	vitro					1470:1474	vitro	1470:1474	vitro	1470:1474	Crystalline mAb-laden particles were evaluated for their biocompatibility using cell-based in vitro assays.					
36745878	9	13	theme	tuning	1290:1295	arg1	disintegration					1321:1334	tuning the alginate particles' disintegration	1290:1334	tuning the alginate particles' disintegration	1290:1334	We illustrated tuning the alginate particles' disintegration by utilizing partially oxide alginates.					
36745878	2	14	theme	high	265:268	arg1	concentrations					270:283	high concentrations	265:283	high concentrations with suitable stability	265:307	However, formulating mAbs for small injection volumes at high concentrations with suitable stability and injectability is a significant challenge.					
36745878	10	15	theme	mAb-laden	1388:1396	arg1	particles					1398:1406	Crystalline mAb-laden particles	1376:1406	Crystalline mAb-laden particles	1376:1406	Crystalline mAb-laden particles were evaluated for their biocompatibility using cell-based in vitro assays.					
36745878	0	16	theme	Concentration	76:88	arg1	Delivery					103:110	High Concentration Subcutaneous Delivery	71:110	High Concentration Subcutaneous Delivery of Antibodies	71:124	Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.					
36745878	11	17	from	pharmacokinetics	1501:1516	arg1	particles					1617:1625	crystalline antibody-laden alginate hydrogel particles	1572:1625	crystalline antibody-laden alginate hydrogel particles in Wistar rats	1572:1640	Furthermore, the pharmacokinetics of the subcutaneously delivered human ant-PD-1 mAb in crystalline antibody-laden alginate hydrogel particles in Wistar rats was evaluated.					
36745878	1	18	theme	monoclonal	178:187	arg1	mAbs					201:204	mAbs	201:204	mAbs	201:204	Subcutaneous administration is a desired route for monoclonal antibodies (mAbs).					
36745878	1	18	theme	monoclonal	178:187	arg1	antibodies					189:198	monoclonal antibodies	178:198	monoclonal antibodies (mAbs)	178:205	Subcutaneous administration is a desired route for monoclonal antibodies (mAbs).					
36745878	3	19	theme	platform	373:380	arg1	technology					382:391	a platform technology	371:391	a platform technology that combines the stability of crystalline antibodies with injectability and tunability of soft hydrogel particles	371:506	Here we present a platform technology that combines the stability of crystalline antibodies with injectability and tunability of soft hydrogel particles.					
36745878	8	20	theme	Bioanalytical	1108:1120	arg1	studies					1122:1128	Bioanalytical studies	1108:1128	Bioanalytical studies	1108:1128	Bioanalytical studies confirmed superior maintained quality of the antibody in comparison with formulation approaches involving organic phases and chemical reactions.					
36745878	4	21	theme	Composite	509:517	arg1	particles					537:545	Composite alginate hydrogel particles	509:545	Composite alginate hydrogel particles	509:545	Composite alginate hydrogel particles were generated via a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase.					
36745878	0	22	theme	Antibodies	115:124	arg1	Delivery					103:110	High Concentration Subcutaneous Delivery	71:110	High Concentration Subcutaneous Delivery of Antibodies	71:124	Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.					
36745878	4	23	theme	hydrogel	528:535	arg1	particles					537:545	Composite alginate hydrogel particles	509:545	Composite alginate hydrogel particles	509:545	Composite alginate hydrogel particles were generated via a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase.					
36745878	11	24	theme	alginate	1599:1606	arg1	particles					1617:1625	crystalline antibody-laden alginate hydrogel particles	1572:1625	crystalline antibody-laden alginate hydrogel particles in Wistar rats	1572:1640	Furthermore, the pharmacokinetics of the subcutaneously delivered human ant-PD-1 mAb in crystalline antibody-laden alginate hydrogel particles in Wistar rats was evaluated.					
36745878	0	25	theme	Crystalline	0:10	arg1	Particles					36:44	Crystalline Antibody-Laden Alginate Particles	0:44	Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.	0:125	Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.					
36745878	8	26	theme	maintained	1149:1158	arg1	quality					1160:1166	superior maintained quality	1140:1166	superior maintained quality of the antibody	1140:1182	Bioanalytical studies confirmed superior maintained quality of the antibody in comparison with formulation approaches involving organic phases and chemical reactions.					
36745878	9	27	theme	alginate	1301:1308	arg1	particles					1310:1318	the alginate particles'	1297:1319	tuning the alginate particles' disintegration	1290:1334	We illustrated tuning the alginate particles' disintegration by utilizing partially oxide alginates.					
36745878	10	28	theme	cell-based	1456:1465	arg1	assays					1476:1481	cell-based in vitro assays	1456:1481	cell-based in vitro assays	1456:1481	Crystalline mAb-laden particles were evaluated for their biocompatibility using cell-based in vitro assays.					
36745878	3	29	theme	particles	498:506	arg1	tunability					470:479	tunability	470:479	tunability	470:479	Here we present a platform technology that combines the stability of crystalline antibodies with injectability and tunability of soft hydrogel particles.					
36745878	3	29	theme	particles	498:506	arg1	injectability					452:464	injectability	452:464	injectability	452:464	Here we present a platform technology that combines the stability of crystalline antibodies with injectability and tunability of soft hydrogel particles.					
36745878	0	30	theme	Alginate	27:34	arg1	Particles					36:44	Crystalline Antibody-Laden Alginate Particles	0:44	Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.	0:125	Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.					
36745878	3	31	theme	antibodies	436:445	arg1	stability					411:419	the stability	407:419	the stability of crystalline antibodies	407:445	Here we present a platform technology that combines the stability of crystalline antibodies with injectability and tunability of soft hydrogel particles.					
36745878	11	32	theme	Wistar	1630:1635	arg1	rats					1637:1640	Wistar rats	1630:1640	Wistar rats	1630:1640	Furthermore, the pharmacokinetics of the subcutaneously delivered human ant-PD-1 mAb in crystalline antibody-laden alginate hydrogel particles in Wistar rats was evaluated.					
36745878	3	33	theme	soft	484:487	arg1	particles					498:506	soft hydrogel particles	484:506	soft hydrogel particles	484:506	Here we present a platform technology that combines the stability of crystalline antibodies with injectability and tunability of soft hydrogel particles.					
36745878	8	34	theme	antibody	1175:1182	arg1	quality					1160:1166	superior maintained quality	1140:1166	superior maintained quality of the antibody	1140:1182	Bioanalytical studies confirmed superior maintained quality of the antibody in comparison with formulation approaches involving organic phases and chemical reactions.					
36745878	4	35	theme	gentle	568:573	arg1	process					601:607	a gentle centrifugal encapsulation process	566:607	a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase	566:675	Composite alginate hydrogel particles were generated via a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase.					
36745878	4	36	theme	encapsulation	587:599	arg1	process					601:607	a gentle centrifugal encapsulation process	566:607	a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase	566:675	Composite alginate hydrogel particles were generated via a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase.					
36745878	6	37	theme	mAb	810:812	arg1	encapsuled					814:823	the mAb encapsuled	806:823	the mAb encapsuled in the hydrogel particles	806:849	Crystalline forms of the mAb encapsuled in the hydrogel particles led to stable, high concentration and injectable formulations.					
36745878	8	38	theme	formulation	1203:1213	arg1	approaches					1215:1224	formulation approaches	1203:1224	formulation approaches involving organic phases and chemical reactions	1203:1272	Bioanalytical studies confirmed superior maintained quality of the antibody in comparison with formulation approaches involving organic phases and chemical reactions.					
36745878	6	39	theme	injectable	889:898	arg1	formulations					900:911	stable, high concentration and injectable formulations	858:911	stable, high concentration and injectable formulations	858:911	Crystalline forms of the mAb encapsuled in the hydrogel particles led to stable, high concentration and injectable formulations.					
36745878	4	40	theme	external	654:661	arg1	phase					671:675	an external organic phase	651:675	an external organic phase	651:675	Composite alginate hydrogel particles were generated via a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase.					
36745878	11	41	theme	delivered	1540:1548	arg1	mAb					1565:1567	the subcutaneously delivered human ant-PD-1 mAb	1521:1567	the subcutaneously delivered human ant-PD-1 mAb	1521:1567	Furthermore, the pharmacokinetics of the subcutaneously delivered human ant-PD-1 mAb in crystalline antibody-laden alginate hydrogel particles in Wistar rats was evaluated.					
36745878	11	42	from	particles	1617:1625	arg1	rats					1637:1640	Wistar rats	1630:1640	Wistar rats	1630:1640	Furthermore, the pharmacokinetics of the subcutaneously delivered human ant-PD-1 mAb in crystalline antibody-laden alginate hydrogel particles in Wistar rats was evaluated.					
36745878	5	43	theme	antibody	714:721	arg1	antibody					775:782	a model therapeutic antibody	755:782	a model therapeutic antibody	755:782	Crystalline suspension of anti-PD-1 antibody (pembrolizumab) was utilized as a model therapeutic antibody.					
36745878	5	43	theme	antibody	714:721	arg1	suspension					690:699	Crystalline suspension	678:699	Crystalline suspension of anti-PD-1 antibody (pembrolizumab)	678:737	Crystalline suspension of anti-PD-1 antibody (pembrolizumab) was utilized as a model therapeutic antibody.					
36745878	6	44	theme	Crystalline	785:795	arg1	forms					797:801	Crystalline forms	785:801	Crystalline forms of the mAb encapsuled in the hydrogel particles	785:849	Crystalline forms of the mAb encapsuled in the hydrogel particles led to stable, high concentration and injectable formulations.					
36745878	4	45	theme	chemical	629:636	arg1	reactions					638:646	chemical reactions	629:646	chemical reactions	629:646	Composite alginate hydrogel particles were generated via a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase.					
36745878	6	46	theme	high	866:869	arg1	concentration					871:883	high concentration	866:883	high concentration	866:883	Crystalline forms of the mAb encapsuled in the hydrogel particles led to stable, high concentration and injectable formulations.					
36745878	7	47	theme	315 mg/ml	952:960	arg1	antibody					962:969	315 mg/ml antibody	952:969	315 mg/ml antibody	952:969	Formulation concentrations as high as 315 mg/ml antibody were achieved with encapsulation efficiencies in the range of 89-97%, with no perceivable increase in the number of antibody aggregates.					
36745878	8	48	theme	chemical	1255:1262	arg1	reactions					1264:1272	chemical reactions	1255:1272	chemical reactions	1255:1272	Bioanalytical studies confirmed superior maintained quality of the antibody in comparison with formulation approaches involving organic phases and chemical reactions.					
36745878	11	49	theme	ant-PD-1	1556:1563	arg1	mAb					1565:1567	the subcutaneously delivered human ant-PD-1 mAb	1521:1567	the subcutaneously delivered human ant-PD-1 mAb	1521:1567	Furthermore, the pharmacokinetics of the subcutaneously delivered human ant-PD-1 mAb in crystalline antibody-laden alginate hydrogel particles in Wistar rats was evaluated.					
36745878	5	50	theme	Crystalline	678:688	arg1	antibody					775:782	a model therapeutic antibody	755:782	a model therapeutic antibody	755:782	Crystalline suspension of anti-PD-1 antibody (pembrolizumab) was utilized as a model therapeutic antibody.					
36745878	5	50	theme	Crystalline	678:688	arg1	suspension					690:699	Crystalline suspension	678:699	Crystalline suspension of anti-PD-1 antibody (pembrolizumab)	678:737	Crystalline suspension of anti-PD-1 antibody (pembrolizumab) was utilized as a model therapeutic antibody.					
36745878	6	51	theme	stable	858:863	arg1	formulations					900:911	stable, high concentration and injectable formulations	858:911	stable, high concentration and injectable formulations	858:911	Crystalline forms of the mAb encapsuled in the hydrogel particles led to stable, high concentration and injectable formulations.					
36745878	2	52	theme	suitable	290:297	arg1	stability					299:307	suitable stability	290:307	suitable stability	290:307	However, formulating mAbs for small injection volumes at high concentrations with suitable stability and injectability is a significant challenge.					
36745878	7	53	theme	high	944:947	arg1	concentrations					926:939	Formulation concentrations	914:939	Formulation concentrations as high as 315 mg/ml antibody	914:969	Formulation concentrations as high as 315 mg/ml antibody were achieved with encapsulation efficiencies in the range of 89-97%, with no perceivable increase in the number of antibody aggregates.					
36745878	2	54	theme	significant	332:342	arg1	challenge					344:352	a significant challenge	330:352	a significant challenge	330:352	However, formulating mAbs for small injection volumes at high concentrations with suitable stability and injectability is a significant challenge.					
36745878	5	55	theme	model	757:761	arg1	antibody					775:782	a model therapeutic antibody	755:782	a model therapeutic antibody	755:782	Crystalline suspension of anti-PD-1 antibody (pembrolizumab) was utilized as a model therapeutic antibody.					
36745878	5	55	theme	model	757:761	arg1	suspension					690:699	Crystalline suspension	678:699	Crystalline suspension of anti-PD-1 antibody (pembrolizumab)	678:737	Crystalline suspension of anti-PD-1 antibody (pembrolizumab) was utilized as a model therapeutic antibody.					
36745878	6	56	theme	hydrogel	832:839	arg1	particles					841:849	the hydrogel particles	828:849	the hydrogel particles	828:849	Crystalline forms of the mAb encapsuled in the hydrogel particles led to stable, high concentration and injectable formulations.					
36745878	2	57	theme	injection	244:252	arg1	volumes					254:260	small injection volumes	238:260	small injection volumes	238:260	However, formulating mAbs for small injection volumes at high concentrations with suitable stability and injectability is a significant challenge.					
36745878	7	58	theme	antibody	1087:1094	arg1	aggregates					1096:1105	antibody aggregates	1087:1105	antibody aggregates	1087:1105	Formulation concentrations as high as 315 mg/ml antibody were achieved with encapsulation efficiencies in the range of 89-97%, with no perceivable increase in the number of antibody aggregates.					
36745878	1	59	theme	Subcutaneous	127:138	arg1	route					168:172	a desired route	158:172	a desired route for monoclonal antibodies (mAbs)	158:205	Subcutaneous administration is a desired route for monoclonal antibodies (mAbs).					
36745878	1	59	theme	Subcutaneous	127:138	arg1	administration					140:153	Subcutaneous administration	127:153	Subcutaneous administration	127:153	Subcutaneous administration is a desired route for monoclonal antibodies (mAbs).					
36745878	2	60	with	concentrations	270:283	arg1	stability					299:307	suitable stability	290:307	suitable stability	290:307	However, formulating mAbs for small injection volumes at high concentrations with suitable stability and injectability is a significant challenge.					
36745878	7	61	theme	aggregates	1096:1105	arg1	number					1077:1082	the number	1073:1082	the number of antibody aggregates	1073:1105	Formulation concentrations as high as 315 mg/ml antibody were achieved with encapsulation efficiencies in the range of 89-97%, with no perceivable increase in the number of antibody aggregates.					
36745878	0	62	theme	High	71:74	arg1	Delivery					103:110	High Concentration Subcutaneous Delivery	71:110	High Concentration Subcutaneous Delivery of Antibodies	71:124	Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.					
36745878	1	63	theme	desired	160:166	arg1	route					168:172	a desired route	158:172	a desired route for monoclonal antibodies (mAbs)	158:205	Subcutaneous administration is a desired route for monoclonal antibodies (mAbs).					
36745878	1	63	theme	desired	160:166	arg1	administration					140:153	Subcutaneous administration	127:153	Subcutaneous administration	127:153	Subcutaneous administration is a desired route for monoclonal antibodies (mAbs).					
36745878	0	64	theme	Subcutaneous	90:101	arg1	Delivery					103:110	High Concentration Subcutaneous Delivery	71:110	High Concentration Subcutaneous Delivery of Antibodies	71:124	Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.					
36745878	7	65	theme	perceivable	1049:1059	arg1	increase					1061:1068	no perceivable increase	1046:1068	no perceivable increase in the number of antibody aggregates	1046:1105	Formulation concentrations as high as 315 mg/ml antibody were achieved with encapsulation efficiencies in the range of 89-97%, with no perceivable increase in the number of antibody aggregates.					
36745878	10	66	theme	Crystalline	1376:1386	arg1	particles					1398:1406	Crystalline mAb-laden particles	1376:1406	Crystalline mAb-laden particles	1376:1406	Crystalline mAb-laden particles were evaluated for their biocompatibility using cell-based in vitro assays.					
36745878	4	67	theme	alginate	519:526	arg1	particles					537:545	Composite alginate hydrogel particles	509:545	Composite alginate hydrogel particles	509:545	Composite alginate hydrogel particles were generated via a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase.					
36745878	11	68	theme	hydrogel	1608:1615	arg1	particles					1617:1625	crystalline antibody-laden alginate hydrogel particles	1572:1625	crystalline antibody-laden alginate hydrogel particles in Wistar rats	1572:1640	Furthermore, the pharmacokinetics of the subcutaneously delivered human ant-PD-1 mAb in crystalline antibody-laden alginate hydrogel particles in Wistar rats was evaluated.					
36745878	8	69	theme	superior	1140:1147	arg1	quality					1160:1166	superior maintained quality	1140:1166	superior maintained quality of the antibody	1140:1182	Bioanalytical studies confirmed superior maintained quality of the antibody in comparison with formulation approaches involving organic phases and chemical reactions.					
36745878	7	70	theme	%	1038:1038	arg1	range					1024:1028	the range	1020:1028	the range of 89-97%	1020:1038	Formulation concentrations as high as 315 mg/ml antibody were achieved with encapsulation efficiencies in the range of 89-97%, with no perceivable increase in the number of antibody aggregates.					
36745878	11	71	theme	antibody-laden	1584:1597	arg1	particles					1617:1625	crystalline antibody-laden alginate hydrogel particles	1572:1625	crystalline antibody-laden alginate hydrogel particles in Wistar rats	1572:1640	Furthermore, the pharmacokinetics of the subcutaneously delivered human ant-PD-1 mAb in crystalline antibody-laden alginate hydrogel particles in Wistar rats was evaluated.					
36745878	0	72	theme	Antibody-Laden	12:25	arg1	Particles					36:44	Crystalline Antibody-Laden Alginate Particles	0:44	Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.	0:125	Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.					
36745878	2	73	dep	challenge	344:352	arg1	formulating					217:227	formulating	217:227	formulating mAbs for small injection volumes at high concentrations with suitable stability	217:307	However, formulating mAbs for small injection volumes at high concentrations with suitable stability and injectability is a significant challenge.					
36745878	10	74	theme	in	1467:1468	arg1	assays					1476:1481	cell-based in vitro assays	1456:1481	cell-based in vitro assays	1456:1481	Crystalline mAb-laden particles were evaluated for their biocompatibility using cell-based in vitro assays.					
36745878	6	75	from	particles	841:849	arg1	forms					797:801	Crystalline forms	785:801	Crystalline forms of the mAb encapsuled in the hydrogel particles	785:849	Crystalline forms of the mAb encapsuled in the hydrogel particles led to stable, high concentration and injectable formulations.					
36745878	7	76	from	efficiencies	1004:1015	arg1	range					1024:1028	the range	1020:1028	the range of 89-97%	1020:1038	Formulation concentrations as high as 315 mg/ml antibody were achieved with encapsulation efficiencies in the range of 89-97%, with no perceivable increase in the number of antibody aggregates.					
36745878	4	77	theme	centrifugal	575:585	arg1	process					601:607	a gentle centrifugal encapsulation process	566:607	a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase	566:675	Composite alginate hydrogel particles were generated via a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase.					
36745878	3	78	theme	hydrogel	489:496	arg1	particles					498:506	soft hydrogel particles	484:506	soft hydrogel particles	484:506	Here we present a platform technology that combines the stability of crystalline antibodies with injectability and tunability of soft hydrogel particles.					
36745878	6	79	from	encapsuled	814:823	arg1	particles					841:849	the hydrogel particles	828:849	the hydrogel particles	828:849	Crystalline forms of the mAb encapsuled in the hydrogel particles led to stable, high concentration and injectable formulations.					
36745878	3	80	theme	crystalline	424:434	arg1	antibodies					436:445	crystalline antibodies	424:445	crystalline antibodies	424:445	Here we present a platform technology that combines the stability of crystalline antibodies with injectability and tunability of soft hydrogel particles.					
36745878	6	81	theme	encapsuled	814:823	arg1	forms					797:801	Crystalline forms	785:801	Crystalline forms of the mAb encapsuled in the hydrogel particles	785:849	Crystalline forms of the mAb encapsuled in the hydrogel particles led to stable, high concentration and injectable formulations.					
36745878	7	82	theme	encapsulation	990:1002	arg1	efficiencies					1004:1015	encapsulation efficiencies	990:1015	encapsulation efficiencies in the range of 89-97%	990:1038	Formulation concentrations as high as 315 mg/ml antibody were achieved with encapsulation efficiencies in the range of 89-97%, with no perceivable increase in the number of antibody aggregates.					
36745878	0	83	dep	Particles	36:44	arg1	Platform					49:56	A Platform	47:56	Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.	0:125	Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.					
36745878	4	84	theme	organic	663:669	arg1	phase					671:675	an external organic phase	651:675	an external organic phase	651:675	Composite alginate hydrogel particles were generated via a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase.					
36745878	8	85	theme	organic	1236:1242	arg1	phases					1244:1249	organic phases	1236:1249	organic phases	1236:1249	Bioanalytical studies confirmed superior maintained quality of the antibody in comparison with formulation approaches involving organic phases and chemical reactions.					
36745878	6	86	from	forms	797:801	arg1	particles					841:849	the hydrogel particles	828:849	the hydrogel particles	828:849	Crystalline forms of the mAb encapsuled in the hydrogel particles led to stable, high concentration and injectable formulations.					
36745878	11	87	theme	human	1550:1554	arg1	mAb					1565:1567	the subcutaneously delivered human ant-PD-1 mAb	1521:1567	the subcutaneously delivered human ant-PD-1 mAb	1521:1567	Furthermore, the pharmacokinetics of the subcutaneously delivered human ant-PD-1 mAb in crystalline antibody-laden alginate hydrogel particles in Wistar rats was evaluated.					
35956930	5	0	theme	extract	799:805	arg1	analysis					777:784	Shotgun proteomics analysis	758:784	Shotgun proteomics analysis of the crude extract	758:805	Shotgun proteomics analysis of the crude extract enabled the identification of two pectin-lyases, one pectate-lyase and a glucosidase.					
35956930	4	1	theme	Polygalacturonase	623:639	arg1	activities					697:706	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities	623:706	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities	623:706	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities were 3.5; 0.08; 3.1 and 0.8 U mL-1, respectively.					
35956930	2	2	theme	composite	476:484	arg1	designs					486:492	two rotational central composite designs	453:492	two rotational central composite designs	453:492	A factorial design was performed to determine the influence variables and two rotational central composite designs were executed.					
35956930	3	3	theme	static	611:616	arg1	SSF					618:620	static SSF	611:620	static SSF	611:620	The validated experimental result was of 7.1 U mL-1 using 50% PFRF (w/w), pH 5, 30 °C for 24 h, under static SSF.					
35956930	6	4	from	values	976:981	arg1	activity					961:968	its original activity	948:968	its original activity	948:968	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	6	4	from	values	976:981	arg1	%					943:943	at least 80%	932:943	at least 80% of its original activity at pH values	932:981	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	6	4	from	values	976:981	arg1	temperatures					987:998	temperatures	987:998	temperatures ranging from 2 to 8 and 30 to 80 °C	987:1034	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	7	5	theme	cost-effective	1113:1126	arg1	PFRF					1097:1100	PFRF	1097:1100	PFRF	1097:1100	Results revealed that PFRF might be a cost-effective and eco-friendly substrate to produce pectinases.					
35956930	7	5	theme	cost-effective	1113:1126	arg1	substrate					1145:1153	a cost-effective and eco-friendly substrate	1111:1153	a cost-effective and eco-friendly substrate to produce pectinases	1111:1175	Results revealed that PFRF might be a cost-effective and eco-friendly substrate to produce pectinases.					
35956930	1	6	theme	Brazilian	268:276	arg1	vat					295:297	Brazilian sugarcane liquor vat	268:297	Brazilian sugarcane liquor vat	268:297	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	3	7	theme	validated	513:521	arg1	result					536:541	The validated experimental result	509:541	The validated experimental result	509:541	The validated experimental result was of 7.1 U mL-1 using 50% PFRF (w/w), pH 5, 30 °C for 24 h, under static SSF.					
35956930	6	8	theme	min	1059:1061	arg1	incubation					1063:1072	60 min incubation	1056:1072	60 min incubation	1056:1072	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	1	9	theme	sugarcane	278:286	arg1	vat					295:297	Brazilian sugarcane liquor vat	268:297	Brazilian sugarcane liquor vat	268:297	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	1	10	theme	solid-state	347:357	arg1	fermentation					359:370	solid-state fermentation	347:370	solid-state fermentation (SSF)	347:376	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	1	10	theme	solid-state	347:357	arg1	SSF					373:375	SSF	373:375	SSF	373:375	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	6	11	theme	enzymatic	903:911	arg1	extract					913:919	The crude enzymatic extract	893:919	The crude enzymatic extract	893:919	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	6	12	theme	60	1056:1057	arg1	min					1059:1061	min	1059:1061	min	1059:1061	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	1	13	theme	liquor	288:293	arg1	vat					295:297	Brazilian sugarcane liquor vat	268:297	Brazilian sugarcane liquor vat	268:297	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	0	14	theme	Proteomics	123:132	arg1	Approach					134:141	a Shotgun Proteomics Approach	113:141	a Shotgun Proteomics Approach	113:141	Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach.					
35956930	0	15	from	Optimization	50:61	arg1	Identification					95:108	Identification	95:108	Identification	95:108	Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach.					
35956930	0	15	from	Optimization	50:61	arg1	Fermentation					78:89	Solid-State Fermentation	66:89	Solid-State Fermentation	66:89	Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach.					
35956930	6	16	theme	activity	961:968	arg1	activity					961:968	its original activity	948:968	its original activity	948:968	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	6	16	theme	activity	961:968	arg1	%					943:943	at least 80%	932:943	at least 80% of its original activity at pH values	932:981	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	6	16	theme	activity	961:968	arg1	temperatures					987:998	temperatures	987:998	temperatures ranging from 2 to 8 and 30 to 80 °C	987:1034	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	8	17	theme	pectin	1242:1247	arg1	proteins					1256:1263	fermentation conditions wherein pectin active proteins	1210:1263	fermentation conditions wherein pectin active proteins predominated	1210:1276	Statistical optimization led to fermentation conditions wherein pectin active proteins predominated.					
35956930	5	18	theme	pectin-lyases	841:853	arg1	identification					819:832	the identification	815:832	the identification of two pectin-lyases, one pectate-lyase and a glucosidase	815:890	Shotgun proteomics analysis of the crude extract enabled the identification of two pectin-lyases, one pectate-lyase and a glucosidase.					
35956930	2	19	theme	factorial	381:389	arg1	design					391:396	A factorial design	379:396	A factorial design	379:396	A factorial design was performed to determine the influence variables and two rotational central composite designs were executed.					
35956930	0	20	theme	Shotgun	115:121	arg1	Approach					134:141	a Shotgun Proteomics Approach	113:141	a Shotgun Proteomics Approach	113:141	Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach.					
35956930	6	21	dep	80	1030:1031	arg1	to					1027:1028	to	1027:1028	to	1027:1028	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	4	22	theme	pectin-lyase	666:677	arg1	activities					697:706	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities	623:706	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities	623:706	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities were 3.5; 0.08; 3.1 and 0.8 U mL-1, respectively.					
35956930	0	23	theme	Pectinases	0:9	arg1	Secretion					11:19	Pectinases Secretion	0:19	Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach.	0:142	Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach.					
35956930	6	24	theme	crude	897:901	arg1	extract					913:919	The crude enzymatic extract	893:919	The crude enzymatic extract	893:919	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	2	25	dep	influence	429:437	arg1	executed					499:506	executed	499:506	were executed	494:506	A factorial design was performed to determine the influence variables and two rotational central composite designs were executed.					
35956930	3	26	theme	50	567:568	arg1	%					569:569	%	569:569	%	569:569	The validated experimental result was of 7.1 U mL-1 using 50% PFRF (w/w), pH 5, 30 °C for 24 h, under static SSF.					
35956930	5	27	theme	crude	793:797	arg1	extract					799:805	the crude extract	789:805	the crude extract	789:805	Shotgun proteomics analysis of the crude extract enabled the identification of two pectin-lyases, one pectate-lyase and a glucosidase.					
35956930	0	28	theme	Saccharomyces	24:36	arg1	cerevisiae					38:47	Saccharomyces cerevisiae	24:47	Saccharomyces cerevisiae	24:47	Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach.					
35956930	3	29	theme	U	554:554	arg1	mL-1					556:559	7.1 U mL-1	550:559	7.1 U mL-1	550:559	The validated experimental result was of 7.1 U mL-1 using 50% PFRF (w/w), pH 5, 30 °C for 24 h, under static SSF.					
35956930	9	30	theme	pectate	1362:1368	arg1	lyase					1370:1374	pectate lyase	1362:1374	pectate lyase	1362:1374	To the extent of our knowledge, this is the first study reporting the synthesis of pectate lyase by S. cerevisiae.					
35956930	9	31	theme	lyase	1370:1374	arg1	synthesis					1349:1357	the synthesis	1345:1357	the synthesis of pectate lyase by S. cerevisiae	1345:1391	To the extent of our knowledge, this is the first study reporting the synthesis of pectate lyase by S. cerevisiae.					
35956930	8	32	theme	conditions	1223:1232	arg1	proteins					1256:1263	fermentation conditions wherein pectin active proteins	1210:1263	fermentation conditions wherein pectin active proteins predominated	1210:1276	Statistical optimization led to fermentation conditions wherein pectin active proteins predominated.					
35956930	9	33	theme	first	1323:1327	arg1	study					1329:1333	the first study	1319:1333	the first study reporting the synthesis of pectate lyase by S. cerevisiae	1319:1391	To the extent of our knowledge, this is the first study reporting the synthesis of pectate lyase by S. cerevisiae.					
35956930	9	33	theme	first	1323:1327	arg1	this					1311:1314	this	1311:1314	this	1311:1314	To the extent of our knowledge, this is the first study reporting the synthesis of pectate lyase by S. cerevisiae.					
35956930	1	34	theme	pectinases	214:223	arg1	secretion					201:209	the secretion	197:209	the secretion of pectinases by a Saccharomyces cerevisiae strain	197:260	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	2	35	theme	central	468:474	arg1	designs					486:492	two rotational central composite designs	453:492	two rotational central composite designs	453:492	A factorial design was performed to determine the influence variables and two rotational central composite designs were executed.					
35956930	1	36	theme	passion	303:309	arg1	PFRF					332:335	PFRF	332:335	PFRF	332:335	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	1	36	theme	passion	303:309	arg1	flour					325:329	passion fruit residue flour	303:329	passion fruit residue flour (PFRF)	303:336	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	6	37	theme	original	952:959	arg1	activity					961:968	its original activity	948:968	its original activity	948:968	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	4	38	theme	U	736:736	arg1	mL-1					738:741	0.8 U mL-1	732:741	0.8 U mL-1	732:741	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities were 3.5; 0.08; 3.1 and 0.8 U mL-1, respectively.					
35956930	6	39	theme	pH	973:974	arg1	values					976:981	pH values	973:981	pH values	973:981	The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation.					
35956930	5	40	theme	Shotgun	758:764	arg1	analysis					777:784	Shotgun proteomics analysis	758:784	Shotgun proteomics analysis of the crude extract	758:805	Shotgun proteomics analysis of the crude extract enabled the identification of two pectin-lyases, one pectate-lyase and a glucosidase.					
35956930	2	41	theme	rotational	457:466	arg1	designs					486:492	two rotational central composite designs	453:492	two rotational central composite designs	453:492	A factorial design was performed to determine the influence variables and two rotational central composite designs were executed.					
35956930	1	42	theme	fruit	311:315	arg1	PFRF					332:335	PFRF	332:335	PFRF	332:335	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	1	42	theme	fruit	311:315	arg1	flour					325:329	passion fruit residue flour	303:329	passion fruit residue flour (PFRF)	303:336	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	1	43	dep	Saccharomyces	230:242	arg1	cerevisiae					244:253	cerevisiae	244:253	cerevisiae	244:253	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	5	44	theme	proteomics	766:775	arg1	analysis					777:784	Shotgun proteomics analysis	758:784	Shotgun proteomics analysis of the crude extract	758:805	Shotgun proteomics analysis of the crude extract enabled the identification of two pectin-lyases, one pectate-lyase and a glucosidase.					
35956930	8	45	theme	Statistical	1178:1188	arg1	optimization					1190:1201	Statistical optimization	1178:1201	Statistical optimization	1178:1201	Statistical optimization led to fermentation conditions wherein pectin active proteins predominated.					
35956930	1	46	theme	residue	317:323	arg1	PFRF					332:335	PFRF	332:335	PFRF	332:335	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	1	46	theme	residue	317:323	arg1	flour					325:329	passion fruit residue flour	303:329	passion fruit residue flour (PFRF)	303:336	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	8	47	theme	active	1249:1254	arg1	proteins					1256:1263	fermentation conditions wherein pectin active proteins	1210:1263	fermentation conditions wherein pectin active proteins predominated	1210:1276	Statistical optimization led to fermentation conditions wherein pectin active proteins predominated.					
35956930	3	48	theme	%	569:569	arg1	°C					592:593	30 °C	589:593	30 °C for 24 h	589:602	The validated experimental result was of 7.1 U mL-1 using 50% PFRF (w/w), pH 5, 30 °C for 24 h, under static SSF.					
35956930	3	48	theme	%	569:569	arg1	w/w					577:579	w/w	577:579	w/w	577:579	The validated experimental result was of 7.1 U mL-1 using 50% PFRF (w/w), pH 5, 30 °C for 24 h, under static SSF.					
35956930	3	48	theme	%	569:569	arg1	pH					583:584	pH 5	583:586	pH 5	583:586	The validated experimental result was of 7.1 U mL-1 using 50% PFRF (w/w), pH 5, 30 °C for 24 h, under static SSF.					
35956930	3	48	theme	%	569:569	arg1	PFRF					571:574	50% PFRF	567:574	50% PFRF (w/w)	567:580	The validated experimental result was of 7.1 U mL-1 using 50% PFRF (w/w), pH 5, 30 °C for 24 h, under static SSF.					
35956930	4	49	theme	pectin	642:647	arg1	esterase					656:663	pectin methyl esterase	642:663	pectin methyl esterase	642:663	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities were 3.5; 0.08; 3.1 and 0.8 U mL-1, respectively.					
35956930	7	50	theme	eco-friendly	1132:1143	arg1	PFRF					1097:1100	PFRF	1097:1100	PFRF	1097:1100	Results revealed that PFRF might be a cost-effective and eco-friendly substrate to produce pectinases.					
35956930	7	50	theme	eco-friendly	1132:1143	arg1	substrate					1145:1153	a cost-effective and eco-friendly substrate	1111:1153	a cost-effective and eco-friendly substrate to produce pectinases	1111:1175	Results revealed that PFRF might be a cost-effective and eco-friendly substrate to produce pectinases.					
35956930	4	51	theme	esterase	656:663	arg1	activities					697:706	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities	623:706	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities	623:706	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities were 3.5; 0.08; 3.1 and 0.8 U mL-1, respectively.					
35956930	4	52	theme	pectate-lyase	683:695	arg1	activities					697:706	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities	623:706	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities	623:706	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities were 3.5; 0.08; 3.1 and 0.8 U mL-1, respectively.					
35956930	1	53	theme	Saccharomyces	230:242	arg1	strain					255:260	a Saccharomyces cerevisiae strain	228:260	a Saccharomyces cerevisiae strain	228:260	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	8	54	theme	fermentation	1210:1221	arg1	proteins					1256:1263	fermentation conditions wherein pectin active proteins	1210:1263	fermentation conditions wherein pectin active proteins predominated	1210:1276	Statistical optimization led to fermentation conditions wherein pectin active proteins predominated.					
35956930	1	55	theme	sequential	146:155	arg1	design					157:162	A sequential design	144:162	A sequential design strategy	144:171	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	8	56	theme	wherein	1234:1240	arg1	proteins					1256:1263	fermentation conditions wherein pectin active proteins	1210:1263	fermentation conditions wherein pectin active proteins predominated	1210:1276	Statistical optimization led to fermentation conditions wherein pectin active proteins predominated.					
35956930	4	57	theme	methyl	649:654	arg1	esterase					656:663	pectin methyl esterase	642:663	pectin methyl esterase	642:663	Polygalacturonase, pectin methyl esterase, pectin-lyase and pectate-lyase activities were 3.5; 0.08; 3.1 and 0.8 U mL-1, respectively.					
35956930	0	58	dep	Secretion	11:19	arg1	Optimization					50:61	Optimization	50:61	Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach.	0:142	Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach.					
35956930	1	59	theme	design	157:162	arg1	strategy					164:171	A sequential design strategy	144:171	A sequential design strategy	144:171	A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF).					
35956930	9	60	theme	knowledge	1300:1308	arg1	extent					1286:1291	the extent	1282:1291	the extent of our knowledge	1282:1308	To the extent of our knowledge, this is the first study reporting the synthesis of pectate lyase by S. cerevisiae.					
35956930	0	61	theme	Solid-State	66:76	arg1	Fermentation					78:89	Solid-State Fermentation	66:89	Solid-State Fermentation	66:89	Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach.					
35956930	3	62	theme	experimental	523:534	arg1	result					536:541	The validated experimental result	509:541	The validated experimental result	509:541	The validated experimental result was of 7.1 U mL-1 using 50% PFRF (w/w), pH 5, 30 °C for 24 h, under static SSF.					
36214127	7	0	theme	potential	1508:1516	arg1	treatment					1518:1526	a potential treatment	1506:1526	a potential treatment for orthopaedic endophytes	1506:1553	CONCLUSION Ti-GEN-Cst-PLGA can effectively inhibit the inflammatory response and promote wound healing, or may be a potential treatment for orthopaedic endophytes.					
36214127	1	1	theme	hydrogel	178:185	arg1	system					211:216	a hydrogel drug-controlled release system	176:216	a hydrogel drug-controlled release system loaded with gentamicin	176:239	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	2	2	theme	polyethylene	486:497	arg1	PEG					507:509	PEG	507:509	PEG	507:509	METHODS Titanium (Ti) surface was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG) was attached to PDA.					
36214127	2	2	theme	polyethylene	486:497	arg1	glycol					499:504	polyethylene glycol	486:504	polyethylene glycol (PEG)	486:510	METHODS Titanium (Ti) surface was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG) was attached to PDA.					
36214127	1	3	theme	controlled	373:382	arg1	system					392:397	the controlled release system	369:397	the controlled release system	369:397	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	6	4	theme	Ti-GEN-Cst-PLGA	1267:1281	arg1	group					1283:1287	the Ti-GEN-Cst-PLGA group	1263:1287	the Ti-GEN-Cst-PLGA group	1263:1287	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	1	5	theme	drug-controlled	187:201	arg1	system					211:216	a hydrogel drug-controlled release system	176:216	a hydrogel drug-controlled release system loaded with gentamicin	176:239	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	5	6	theme	assay	977:981	arg1	results					879:885	The results	875:885	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay	875:1019	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay showed that Ti-GEN-Cst-PLGA could effectively inhibit the growth of three bacteria.					
36214127	0	7	theme	anti-infective	120:133	arg1	drugs					135:139	anti-infective drugs	120:139	anti-infective drugs	120:139	Titanium surface polyethylene glycol hydrogel and gentamicin-loaded cross-linked starch microspheres release system for anti-infective drugs.					
36214127	1	8	theme	release	384:390	arg1	system					392:397	the controlled release system	369:397	the controlled release system	369:397	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	5	9	theme	assay	1015:1019	arg1	results					879:885	The results	875:885	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay	875:1019	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay showed that Ti-GEN-Cst-PLGA could effectively inhibit the growth of three bacteria.					
36214127	5	10	theme	epidermidis	959:969	arg1	assay					977:981	anti-staphylococcus epidermidis (SEP) assay	939:981	anti-staphylococcus epidermidis (SEP) assay	939:981	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay showed that Ti-GEN-Cst-PLGA could effectively inhibit the growth of three bacteria.					
36214127	1	11	theme	in	284:285	arg1	behaviour					306:314	the in vitro drug release behaviour	280:314	the in vitro drug release behaviour	280:314	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	5	12	theme	assay	932:936	arg1	results					879:885	The results	875:885	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay	875:1019	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay showed that Ti-GEN-Cst-PLGA could effectively inhibit the growth of three bacteria.					
36214127	1	13	theme	system	392:397	arg1	behaviour					306:314	the in vitro drug release behaviour	280:314	the in vitro drug release behaviour	280:314	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	1	13	theme	system	392:397	arg1	properties					334:343	antibacterial properties	320:343	antibacterial properties	320:343	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	1	13	theme	system	392:397	arg1	biocompatibility					349:364	biocompatibility	349:364	biocompatibility of the controlled release system	349:397	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	6	14	theme	wound	1178:1182	arg1	healing					1184:1190	wound healing	1178:1190	wound healing	1178:1190	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	3	15	theme	composite	537:545	arg1	layer					583:587	The composite drug microsphere controlled release layer	533:587	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt)	533:644	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	3	15	theme	composite	537:545	arg1	barrier					718:724	a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	716:801	a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	716:801	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	3	16	theme	anti-infective	757:770	arg1	system					796:801	a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	739:801	a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	739:801	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	5	17	theme	bacteria	1095:1102	arg1	growth					1079:1084	the growth	1075:1084	the growth of three bacteria	1075:1102	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay showed that Ti-GEN-Cst-PLGA could effectively inhibit the growth of three bacteria.					
36214127	7	18	theme	inflammatory	1447:1458	arg1	response					1460:1467	the inflammatory response	1443:1467	the inflammatory response	1443:1467	CONCLUSION Ti-GEN-Cst-PLGA can effectively inhibit the inflammatory response and promote wound healing, or may be a potential treatment for orthopaedic endophytes.					
36214127	1	19	theme	release	203:209	arg1	system					211:216	a hydrogel drug-controlled release system	176:216	a hydrogel drug-controlled release system loaded with gentamicin	176:239	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	5	20	theme	anti-staphylococcus	899:917	arg1	assay					932:936	in vitro anti-staphylococcus aureus (SAU) assay	890:936	in vitro anti-staphylococcus aureus (SAU) assay	890:936	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay showed that Ti-GEN-Cst-PLGA could effectively inhibit the growth of three bacteria.					
36214127	4	21	theme	release	830:836	arg1	system					838:843	The hydrogel drug release system	812:843	The hydrogel drug release system	812:843	RESULTS The hydrogel drug release system was successfully constructed.					
36214127	0	22	link	cross-linked	68:79	arg1	microspheres					88:99	gentamicin-loaded cross-linked starch microspheres	50:99	gentamicin-loaded cross-linked starch microspheres	50:99	Titanium surface polyethylene glycol hydrogel and gentamicin-loaded cross-linked starch microspheres release system for anti-infective drugs.					
36214127	2	23	theme	PDA	461:463	arg1	substrate					466:474	poly dopamine (PDA) substrate	446:474	poly dopamine (PDA) substrate	446:474	METHODS Titanium (Ti) surface was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG) was attached to PDA.					
36214127	0	24	theme	surface	9:15	arg1	hydrogel					37:44	Titanium surface polyethylene glycol hydrogel	0:44	Titanium surface polyethylene glycol hydrogel	0:44	Titanium surface polyethylene glycol hydrogel and gentamicin-loaded cross-linked starch microspheres release system for anti-infective drugs.					
36214127	6	25	theme	pathology	1236:1244	arg1	assay					1246:1250	the pathology assay	1232:1250	the pathology assay	1232:1250	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	5	26	theme	coli	1004:1007	arg1	assay					1015:1019	anti-Escherichia coli (ECO) assay	987:1019	anti-Escherichia coli (ECO) assay	987:1019	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay showed that Ti-GEN-Cst-PLGA could effectively inhibit the growth of three bacteria.					
36214127	3	27	theme	controlled	564:573	arg1	layer					583:587	The composite drug microsphere controlled release layer	533:587	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt)	533:644	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	3	27	theme	controlled	564:573	arg1	barrier					718:724	a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	716:801	a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	716:801	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	2	28	theme	Ti	418:419	arg1	surface					422:428	Titanium (Ti) surface	408:428	Titanium (Ti) surface	408:428	METHODS Titanium (Ti) surface was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG) was attached to PDA.					
36214127	0	29	theme	Titanium	0:7	arg1	hydrogel					37:44	Titanium surface polyethylene glycol hydrogel	0:44	Titanium surface polyethylene glycol hydrogel	0:44	Titanium surface polyethylene glycol hydrogel and gentamicin-loaded cross-linked starch microspheres release system for anti-infective drugs.					
36214127	1	30	theme	drug	293:296	arg1	behaviour					306:314	the in vitro drug release behaviour	280:314	the in vitro drug release behaviour	280:314	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	6	31	theme	Zealand	1122:1128	arg1	rabbit					1130:1135	the New Zealand rabbit	1114:1135	the New Zealand rabbit	1114:1135	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	3	32	theme	release	575:581	arg1	layer					583:587	The composite drug microsphere controlled release layer	533:587	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt)	533:644	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	3	32	theme	release	575:581	arg1	barrier					718:724	a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	716:801	a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	716:801	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	7	33	theme	orthopaedic	1532:1542	arg1	endophytes					1544:1553	orthopaedic endophytes	1532:1553	orthopaedic endophytes	1532:1553	CONCLUSION Ti-GEN-Cst-PLGA can effectively inhibit the inflammatory response and promote wound healing, or may be a potential treatment for orthopaedic endophytes.					
36214127	0	34	theme	glycol	30:35	arg1	hydrogel					37:44	Titanium surface polyethylene glycol hydrogel	0:44	Titanium surface polyethylene glycol hydrogel	0:44	Titanium surface polyethylene glycol hydrogel and gentamicin-loaded cross-linked starch microspheres release system for anti-infective drugs.					
36214127	1	35	theme	release	298:304	arg1	behaviour					306:314	the in vitro drug release behaviour	280:314	the in vitro drug release behaviour	280:314	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	3	36	theme	drug	547:550	arg1	layer					583:587	The composite drug microsphere controlled release layer	533:587	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt)	533:644	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	3	36	theme	drug	547:550	arg1	barrier					718:724	a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	716:801	a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	716:801	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	2	37	theme	poly	446:449	arg1	substrate					466:474	poly dopamine (PDA) substrate	446:474	poly dopamine (PDA) substrate	446:474	METHODS Titanium (Ti) surface was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG) was attached to PDA.					
36214127	0	38	theme	polyethylene	17:28	arg1	hydrogel					37:44	Titanium surface polyethylene glycol hydrogel	0:44	Titanium surface polyethylene glycol hydrogel	0:44	Titanium surface polyethylene glycol hydrogel and gentamicin-loaded cross-linked starch microspheres release system for anti-infective drugs.					
36214127	6	39	theme	tissue	1337:1342	arg1	proliferation					1344:1356	significant tissue proliferation	1325:1356	significant tissue proliferation	1325:1356	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	3	40	theme	microsphere	552:562	arg1	layer					583:587	The composite drug microsphere controlled release layer	533:587	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt)	533:644	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	3	40	theme	microsphere	552:562	arg1	barrier					718:724	a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	716:801	a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	716:801	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	6	41	theme	significant	1325:1335	arg1	proliferation					1344:1356	significant tissue proliferation	1325:1356	significant tissue proliferation	1325:1356	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	7	42	theme	CONCLUSION	1392:1401	arg1	Ti-GEN-Cst-PLGA					1403:1417	CONCLUSION Ti-GEN-Cst-PLGA	1392:1417	CONCLUSION Ti-GEN-Cst-PLGA	1392:1417	CONCLUSION Ti-GEN-Cst-PLGA can effectively inhibit the inflammatory response and promote wound healing, or may be a potential treatment for orthopaedic endophytes.					
36214127	1	43	theme	antibacterial	320:332	arg1	properties					334:343	antibacterial properties	320:343	antibacterial properties	320:343	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	3	44	theme	cross-linked	620:631	arg1	CSt					641:643	CSt	641:643	CSt	641:643	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	3	44	theme	cross-linked	620:631	arg1	starch					633:638	cross-linked starch	620:638	cross-linked starch (CSt)	620:644	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	2	45	attach	attached	516:523	arg1	PDA					528:530	PDA	528:530	PDA	528:530	METHODS Titanium (Ti) surface was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG) was attached to PDA.					
36214127	2	45	attach	attached	516:523	arg2	METHODS					400:406	METHODS	400:406	METHODS Titanium (Ti) surface was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG)	400:510	METHODS Titanium (Ti) surface was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG) was attached to PDA.					
36214127	1	46	theme	titanium	246:253	arg1	surface					255:261	a titanium surface	244:261	a titanium surface	244:261	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	0	47	theme	cross-linked	68:79	arg1	microspheres					88:99	gentamicin-loaded cross-linked starch microspheres	50:99	gentamicin-loaded cross-linked starch microspheres	50:99	Titanium surface polyethylene glycol hydrogel and gentamicin-loaded cross-linked starch microspheres release system for anti-infective drugs.					
36214127	3	48	theme	drug	772:775	arg1	system					796:801	a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	739:801	a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	739:801	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	6	49	theme	contact	1375:1381	arg1	surface					1383:1389	the endophyte contact surface	1361:1389	the endophyte contact surface	1361:1389	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	4	50	theme	drug	825:828	arg1	system					838:843	The hydrogel drug release system	812:843	The hydrogel drug release system	812:843	RESULTS The hydrogel drug release system was successfully constructed.					
36214127	7	51	theme	wound	1481:1485	arg1	healing					1487:1493	wound healing	1481:1493	wound healing	1481:1493	CONCLUSION Ti-GEN-Cst-PLGA can effectively inhibit the inflammatory response and promote wound healing, or may be a potential treatment for orthopaedic endophytes.					
36214127	5	52	theme	aureus	919:924	arg1	assay					932:936	in vitro anti-staphylococcus aureus (SAU) assay	890:936	in vitro anti-staphylococcus aureus (SAU) assay	890:936	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay showed that Ti-GEN-Cst-PLGA could effectively inhibit the growth of three bacteria.					
36214127	0	53	theme	gentamicin-loaded	50:66	arg1	microspheres					88:99	gentamicin-loaded cross-linked starch microspheres	50:99	gentamicin-loaded cross-linked starch microspheres	50:99	Titanium surface polyethylene glycol hydrogel and gentamicin-loaded cross-linked starch microspheres release system for anti-infective drugs.					
36214127	6	54	theme	inflammatory	1298:1309	arg1	reactions					1311:1319	less inflammatory reactions	1293:1319	less inflammatory reactions	1293:1319	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	1	55	dep	in	284:285	arg1	vitro					287:291	vitro	287:291	vitro	287:291	OBJECTIVE To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.					
36214127	4	56	theme	hydrogel	816:823	arg1	system					838:843	The hydrogel drug release system	812:843	The hydrogel drug release system	812:843	RESULTS The hydrogel drug release system was successfully constructed.					
36214127	6	57	theme	New	1118:1120	arg1	rabbit					1130:1135	the New Zealand rabbit	1114:1135	the New Zealand rabbit	1114:1135	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	3	58	theme	Ti-GEN-Cst-PLGA	741:755	arg1	system					796:801	a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	739:801	a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	739:801	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	5	59	dep	in	890:891	arg1	vitro					893:897	vitro	893:897	vitro	893:897	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay showed that Ti-GEN-Cst-PLGA could effectively inhibit the growth of three bacteria.					
36214127	5	60	theme	SAU	927:929	arg1	assay					932:936	in vitro anti-staphylococcus aureus (SAU) assay	890:936	in vitro anti-staphylococcus aureus (SAU) assay	890:936	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay showed that Ti-GEN-Cst-PLGA could effectively inhibit the growth of three bacteria.					
36214127	2	61	dep	METHODS	400:406	arg1	coated					434:439	coated	434:439	was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG)	430:510	METHODS Titanium (Ti) surface was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG) was attached to PDA.					
36214127	6	62	theme	3rd	1199:1201	arg1	week					1203:1206	the 3rd week	1195:1206	the 3rd week after implantation	1195:1225	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	6	63	theme	endophyte	1365:1373	arg1	surface					1383:1389	the endophyte contact surface	1361:1389	the endophyte contact surface	1361:1389	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	4	64	dep	RESULTS	804:810	arg1	constructed					862:872	constructed	862:872	was successfully constructed	845:872	RESULTS The hydrogel drug release system was successfully constructed.					
36214127	3	65	theme	poly	677:680	arg1	PLGA					707:710	PLGA	707:710	PLGA	707:710	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	3	65	theme	poly	677:680	arg1	acid					701:704	poly lactic⁃co⁃glycolic acid	677:704	poly lactic⁃co⁃glycolic acid (PLGA)	677:711	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	6	66	contain	had	1289:1291	arg2	proliferation					1344:1356	significant tissue proliferation	1325:1356	significant tissue proliferation	1325:1356	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	6	66	contain	had	1289:1291	arg2	reactions					1311:1319	less inflammatory reactions	1293:1319	less inflammatory reactions	1293:1319	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	6	66	contain	had	1289:1291	arg1	group					1283:1287	the Ti-GEN-Cst-PLGA group	1263:1287	the Ti-GEN-Cst-PLGA group	1263:1287	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	2	67	theme	Titanium	408:415	arg1	surface					422:428	Titanium (Ti) surface	408:428	Titanium (Ti) surface	408:428	METHODS Titanium (Ti) surface was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG) was attached to PDA.					
36214127	3	68	theme	controlled	777:786	arg1	system					796:801	a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	739:801	a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	739:801	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	0	69	theme	starch	81:86	arg1	microspheres					88:99	gentamicin-loaded cross-linked starch microspheres	50:99	gentamicin-loaded cross-linked starch microspheres	50:99	Titanium surface polyethylene glycol hydrogel and gentamicin-loaded cross-linked starch microspheres release system for anti-infective drugs.					
36214127	3	70	theme	lactic⁃co⁃glycolic	682:699	arg1	PLGA					707:710	PLGA	707:710	PLGA	707:710	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	3	70	theme	lactic⁃co⁃glycolic	682:699	arg1	acid					701:704	poly lactic⁃co⁃glycolic acid	677:704	poly lactic⁃co⁃glycolic acid (PLGA)	677:711	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	2	71	theme	dopamine	451:458	arg1	substrate					466:474	poly dopamine (PDA) substrate	446:474	poly dopamine (PDA) substrate	446:474	METHODS Titanium (Ti) surface was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG) was attached to PDA.					
36214127	6	72	from	Assay	1105:1109	arg1	rabbit					1130:1135	the New Zealand rabbit	1114:1135	the New Zealand rabbit	1114:1135	Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.					
36214127	5	73	theme	in	890:891	arg1	assay					932:936	in vitro anti-staphylococcus aureus (SAU) assay	890:936	in vitro anti-staphylococcus aureus (SAU) assay	890:936	The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay showed that Ti-GEN-Cst-PLGA could effectively inhibit the growth of three bacteria.					
36214127	3	74	link	cross-linked	620:631	arg1	CSt					641:643	CSt	641:643	CSt	641:643	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	3	74	link	cross-linked	620:631	arg1	starch					633:638	cross-linked starch	620:638	cross-linked starch (CSt)	620:644	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
36214127	3	75	theme	release	788:794	arg1	system					796:801	a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	739:801	a Ti-GEN-Cst-PLGA anti-infective drug controlled release system	739:801	The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.					
35468281	6	0	theme	powerful	1075:1082	arg1	antioxidant					1084:1094	a powerful antioxidant	1073:1094	a powerful antioxidant activity to the pullulan/gelatin-based film	1073:1138	The addition of SNP provided the antimicrobial function against foodborne pathogenic bacteria, L. monocytogenes and E. coli, while GSE provided a powerful antioxidant activity to the pullulan/gelatin-based film.					
35468281	1	1	theme	grape	269:273	arg1	GSE					289:291	GSE	289:291	GSE	289:291	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	1	1	theme	grape	269:273	arg1	extract					280:286	grape seed extract	269:286	grape seed extract (GSE)	269:292	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	0	2	theme	Seed	90:93	arg1	Extract					95:101	Grape Seed Extract	84:101	Grape Seed Extract	84:101	Fabrication of Antioxidant and Antimicrobial Pullulan/Gelatin Films Integrated with Grape Seed Extract and Sulfur Nanoparticles.					
35468281	1	3	with	pullulan	189:196	arg1	fillers					226:232	functional fillers	215:232	functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE)	215:292	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	6	4	theme	antioxidant	1084:1094	arg1	activity					1096:1103	a powerful antioxidant activity	1073:1103	a powerful antioxidant activity to the pullulan/gelatin-based film	1073:1138	The addition of SNP provided the antimicrobial function against foodborne pathogenic bacteria, L. monocytogenes and E. coli, while GSE provided a powerful antioxidant activity to the pullulan/gelatin-based film.					
35468281	2	5	theme	compatible	342:351	arg1	film					392:395	a compatible but slightly translucent free-standing film	340:395	a compatible but slightly translucent free-standing film	340:395	A mixture of pullulan/gelatin (1:1) produced a compatible but slightly translucent free-standing film.					
35468281	1	6	theme	seed	275:278	arg1	GSE					289:291	GSE	289:291	GSE	289:291	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	1	6	theme	seed	275:278	arg1	extract					280:286	grape seed extract	269:286	grape seed extract (GSE)	269:292	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	0	7	theme	Sulfur	107:112	arg1	Nanoparticles					114:126	Sulfur Nanoparticles	107:126	Sulfur Nanoparticles	107:126	Fabrication of Antioxidant and Antimicrobial Pullulan/Gelatin Films Integrated with Grape Seed Extract and Sulfur Nanoparticles.					
35468281	7	8	theme	active	1341:1346	arg1	applications					1363:1374	active food packaging applications	1341:1374	active food packaging applications	1341:1374	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	9	with	films	1185:1189	arg1	properties					1239:1248	oxygen barrier properties	1224:1248	oxygen barrier properties	1224:1248	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	9	with	films	1185:1189	arg1	vapor					1213:1217	water vapor	1207:1217	water vapor	1207:1217	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	9	with	films	1185:1189	arg1	UV					1203:1204	better UV	1196:1204	better UV	1196:1204	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	4	10	theme	films	725:729	arg1	properties					690:699	the UV-light barrier, water vapor barrier, and oxygen barrier properties	628:699	the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films	628:729	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35468281	1	11	theme	extract	280:286	arg1	fillers					226:232	functional fillers	215:232	functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE)	215:292	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	5	12	dep	decreased	808:816	arg1	%					822:822	∼10%	819:822	∼10%	819:822	The mechanical performance of the pullulan/gelatin-based films was slightly decreased (∼10%), whereas the addition of fillers did not significantly affect the hydrophobicity and thermal stability.					
35468281	7	13	with	antioxidant	1263:1273	arg1	properties					1239:1248	oxygen barrier properties	1224:1248	oxygen barrier properties	1224:1248	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	13	with	antioxidant	1263:1273	arg1	vapor					1213:1217	water vapor	1207:1217	water vapor	1207:1217	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	13	with	antioxidant	1263:1273	arg1	UV					1203:1204	better UV	1196:1204	better UV	1196:1204	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	3	14	theme	pullulan/gelatin-based	538:559	arg1	film					561:564	the pullulan/gelatin-based film	534:564	the pullulan/gelatin-based film	534:564	SNPs capped with enoki mushroom extract and GSE were added as functional fillers to improve the properties (physical and functional) of the pullulan/gelatin-based film.					
35468281	5	15	theme	thermal	910:916	arg1	stability					918:926	thermal stability	910:926	thermal stability	910:926	The mechanical performance of the pullulan/gelatin-based films was slightly decreased (∼10%), whereas the addition of fillers did not significantly affect the hydrophobicity and thermal stability.					
35468281	4	16	theme	barrier	682:688	arg1	properties					690:699	the UV-light barrier, water vapor barrier, and oxygen barrier properties	628:699	the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films	628:729	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35468281	7	17	theme	high	1325:1328	arg1	utility					1330:1336	high utility	1325:1336	high utility	1325:1336	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	4	18	theme	barrier	641:647	arg1	properties					690:699	the UV-light barrier, water vapor barrier, and oxygen barrier properties	628:699	the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films	628:729	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35468281	6	19	theme	antimicrobial	962:974	arg1	function					976:983	the antimicrobial function	958:983	the antimicrobial function	958:983	The addition of SNP provided the antimicrobial function against foodborne pathogenic bacteria, L. monocytogenes and E. coli, while GSE provided a powerful antioxidant activity to the pullulan/gelatin-based film.					
35468281	3	20	theme	enoki	415:419	arg1	extract					430:436	enoki mushroom extract	415:436	enoki mushroom extract	415:436	SNPs capped with enoki mushroom extract and GSE were added as functional fillers to improve the properties (physical and functional) of the pullulan/gelatin-based film.					
35468281	4	21	theme	UV-light	632:639	arg1	barrier					641:647	UV-light barrier	632:647	UV-light barrier	632:647	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35468281	4	22	theme	SNP	583:585	arg1	addition					571:578	The addition	567:578	The addition of SNP and GSE	567:593	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35468281	7	23	with	properties	1293:1302	arg1	properties					1239:1248	oxygen barrier properties	1224:1248	oxygen barrier properties	1224:1248	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	23	with	properties	1293:1302	arg1	vapor					1213:1217	water vapor	1207:1217	water vapor	1207:1217	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	23	with	properties	1293:1302	arg1	UV					1203:1204	better UV	1196:1204	better UV	1196:1204	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	24	theme	oxygen	1224:1229	arg1	properties					1239:1248	oxygen barrier properties	1224:1248	oxygen barrier properties	1224:1248	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	4	25	theme	pullulan/gelatin	708:723	arg1	films					725:729	the pullulan/gelatin films	704:729	the pullulan/gelatin films	704:729	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35468281	7	26	theme	antibacterial	1279:1291	arg1	properties					1293:1302	antibacterial properties	1279:1302	antibacterial properties	1279:1302	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	4	27	theme	water	650:654	arg1	barrier					662:668	water vapor barrier	650:668	water vapor barrier	650:668	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35468281	7	28	theme	better	1196:1201	arg1	UV					1203:1204	better UV	1196:1204	better UV	1196:1204	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	29	theme	pullulan/gelatin-based	1152:1173	arg1	films					1185:1189	pullulan/gelatin-based composite films	1152:1189	pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties	1152:1248	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	2	30	theme	pullulan/gelatin	308:323	arg1	mixture					297:303	A mixture	295:303	A mixture of pullulan/gelatin (1:1)	295:329	A mixture of pullulan/gelatin (1:1) produced a compatible but slightly translucent free-standing film.					
35468281	6	31	theme	foodborne	993:1001	arg1	bacteria					1014:1021	foodborne pathogenic bacteria	993:1021	foodborne pathogenic bacteria	993:1021	The addition of SNP provided the antimicrobial function against foodborne pathogenic bacteria, L. monocytogenes and E. coli, while GSE provided a powerful antioxidant activity to the pullulan/gelatin-based film.					
35468281	6	31	theme	foodborne	993:1001	arg1	monocytogenes					1027:1039	L. monocytogenes	1024:1039	L. monocytogenes	1024:1039	The addition of SNP provided the antimicrobial function against foodborne pathogenic bacteria, L. monocytogenes and E. coli, while GSE provided a powerful antioxidant activity to the pullulan/gelatin-based film.					
35468281	6	31	theme	foodborne	993:1001	arg1	coli					1048:1051	E. coli	1045:1051	E. coli	1045:1051	The addition of SNP provided the antimicrobial function against foodborne pathogenic bacteria, L. monocytogenes and E. coli, while GSE provided a powerful antioxidant activity to the pullulan/gelatin-based film.					
35468281	2	32	theme	free-standing	378:390	arg1	film					392:395	a compatible but slightly translucent free-standing film	340:395	a compatible but slightly translucent free-standing film	340:395	A mixture of pullulan/gelatin (1:1) produced a compatible but slightly translucent free-standing film.					
35468281	7	33	contain	have	1320:1323	arg1	films					1185:1189	pullulan/gelatin-based composite films	1152:1189	pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties	1152:1248	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	33	contain	have	1320:1323	arg1	antioxidant					1263:1273	antioxidant	1263:1273	antioxidant	1263:1273	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	33	contain	have	1320:1323	arg2	utility					1330:1336	high utility	1325:1336	high utility	1325:1336	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	34	theme	enhanced	1254:1261	arg1	antioxidant					1263:1273	antioxidant	1263:1273	antioxidant	1263:1273	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	3	35	theme	film	561:564	arg1	properties					494:503	the properties	490:503	the properties (physical and functional) of the pullulan/gelatin-based film	490:564	SNPs capped with enoki mushroom extract and GSE were added as functional fillers to improve the properties (physical and functional) of the pullulan/gelatin-based film.					
35468281	3	36	theme	functional	460:469	arg1	SNPs					398:401	SNPs	398:401	SNPs capped with enoki mushroom extract and GSE	398:444	SNPs capped with enoki mushroom extract and GSE were added as functional fillers to improve the properties (physical and functional) of the pullulan/gelatin-based film.					
35468281	3	36	theme	functional	460:469	arg1	fillers					471:477	functional fillers	460:477	functional fillers	460:477	SNPs capped with enoki mushroom extract and GSE were added as functional fillers to improve the properties (physical and functional) of the pullulan/gelatin-based film.					
35468281	0	37	theme	Antioxidant	15:25	arg1	Films					62:66	Antioxidant and Antimicrobial Pullulan/Gelatin Films	15:66	Antioxidant and Antimicrobial Pullulan/Gelatin Films	15:66	Fabrication of Antioxidant and Antimicrobial Pullulan/Gelatin Films Integrated with Grape Seed Extract and Sulfur Nanoparticles.					
35468281	6	38	theme	SNP	945:947	arg1	addition					933:940	The addition	929:940	The addition of SNP	929:947	The addition of SNP provided the antimicrobial function against foodborne pathogenic bacteria, L. monocytogenes and E. coli, while GSE provided a powerful antioxidant activity to the pullulan/gelatin-based film.					
35468281	3	39	theme	physical	506:513	arg1	properties					494:503	the properties	490:503	the properties (physical and functional) of the pullulan/gelatin-based film	490:564	SNPs capped with enoki mushroom extract and GSE were added as functional fillers to improve the properties (physical and functional) of the pullulan/gelatin-based film.					
35468281	4	40	theme	barrier	662:668	arg1	properties					690:699	the UV-light barrier, water vapor barrier, and oxygen barrier properties	628:699	the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films	628:729	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35468281	0	41	theme	Pullulan/Gelatin	45:60	arg1	Films					62:66	Antioxidant and Antimicrobial Pullulan/Gelatin Films	15:66	Antioxidant and Antimicrobial Pullulan/Gelatin Films	15:66	Fabrication of Antioxidant and Antimicrobial Pullulan/Gelatin Films Integrated with Grape Seed Extract and Sulfur Nanoparticles.					
35468281	4	42	theme	oxygen	675:680	arg1	barrier					682:688	oxygen barrier	675:688	oxygen barrier	675:688	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35468281	6	43	theme	pullulan/gelatin-based	1112:1133	arg1	film					1135:1138	the pullulan/gelatin-based film	1108:1138	the pullulan/gelatin-based film	1108:1138	The addition of SNP provided the antimicrobial function against foodborne pathogenic bacteria, L. monocytogenes and E. coli, while GSE provided a powerful antioxidant activity to the pullulan/gelatin-based film.					
35468281	1	44	theme	functional	215:224	arg1	fillers					226:232	functional fillers	215:232	functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE)	215:292	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	1	45	theme	sulfur	237:242	arg1	SNPs					259:262	SNPs	259:262	SNPs	259:262	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	1	45	theme	sulfur	237:242	arg1	nanoparticles					244:256	sulfur nanoparticles	237:256	sulfur nanoparticles (SNPs)	237:263	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	0	46	theme	Antimicrobial	31:43	arg1	Films					62:66	Antioxidant and Antimicrobial Pullulan/Gelatin Films	15:66	Antioxidant and Antimicrobial Pullulan/Gelatin Films	15:66	Fabrication of Antioxidant and Antimicrobial Pullulan/Gelatin Films Integrated with Grape Seed Extract and Sulfur Nanoparticles.					
35468281	3	47	theme	mushroom	421:428	arg1	extract					430:436	enoki mushroom extract	415:436	enoki mushroom extract	415:436	SNPs capped with enoki mushroom extract and GSE were added as functional fillers to improve the properties (physical and functional) of the pullulan/gelatin-based film.					
35468281	6	48	theme	pathogenic	1003:1012	arg1	bacteria					1014:1021	foodborne pathogenic bacteria	993:1021	foodborne pathogenic bacteria	993:1021	The addition of SNP provided the antimicrobial function against foodborne pathogenic bacteria, L. monocytogenes and E. coli, while GSE provided a powerful antioxidant activity to the pullulan/gelatin-based film.					
35468281	6	48	theme	pathogenic	1003:1012	arg1	monocytogenes					1027:1039	L. monocytogenes	1024:1039	L. monocytogenes	1024:1039	The addition of SNP provided the antimicrobial function against foodborne pathogenic bacteria, L. monocytogenes and E. coli, while GSE provided a powerful antioxidant activity to the pullulan/gelatin-based film.					
35468281	6	48	theme	pathogenic	1003:1012	arg1	coli					1048:1051	E. coli	1045:1051	E. coli	1045:1051	The addition of SNP provided the antimicrobial function against foodborne pathogenic bacteria, L. monocytogenes and E. coli, while GSE provided a powerful antioxidant activity to the pullulan/gelatin-based film.					
35468281	1	49	theme	nanoparticles	244:256	arg1	fillers					226:232	functional fillers	215:232	functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE)	215:292	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	5	50	theme	films	789:793	arg1	performance					747:757	The mechanical performance	732:757	The mechanical performance of the pullulan/gelatin-based films	732:793	The mechanical performance of the pullulan/gelatin-based films was slightly decreased (∼10%), whereas the addition of fillers did not significantly affect the hydrophobicity and thermal stability.					
35468281	7	51	theme	water	1207:1211	arg1	vapor					1213:1217	water vapor	1207:1217	water vapor	1207:1217	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	7	52	theme	barrier	1231:1237	arg1	properties					1239:1248	oxygen barrier properties	1224:1248	oxygen barrier properties	1224:1248	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	5	53	theme	mechanical	736:745	arg1	performance					747:757	The mechanical performance	732:757	The mechanical performance of the pullulan/gelatin-based films	732:793	The mechanical performance of the pullulan/gelatin-based films was slightly decreased (∼10%), whereas the addition of fillers did not significantly affect the hydrophobicity and thermal stability.					
35468281	1	54	theme	Biopolymer-based	129:144	arg1	films					163:167	Biopolymer-based functional blend films	129:167	Biopolymer-based functional blend films	129:167	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	0	55	theme	Films	62:66	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of Antioxidant and Antimicrobial Pullulan/Gelatin Films	0:66	Fabrication of Antioxidant and Antimicrobial Pullulan/Gelatin Films Integrated with Grape Seed Extract and Sulfur Nanoparticles.					
35468281	1	56	theme	functional	146:155	arg1	films					163:167	Biopolymer-based functional blend films	129:167	Biopolymer-based functional blend films	129:167	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	7	57	theme	composite	1175:1183	arg1	films					1185:1189	pullulan/gelatin-based composite films	1152:1189	pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties	1152:1248	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	1	58	theme	blend	157:161	arg1	films					163:167	Biopolymer-based functional blend films	129:167	Biopolymer-based functional blend films	129:167	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	5	59	theme	fillers	850:856	arg1	addition					838:845	the addition	834:845	the addition of fillers	834:856	The mechanical performance of the pullulan/gelatin-based films was slightly decreased (∼10%), whereas the addition of fillers did not significantly affect the hydrophobicity and thermal stability.					
35468281	1	60	with	gelatin	202:208	arg1	fillers					226:232	functional fillers	215:232	functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE)	215:292	Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE).					
35468281	4	61	theme	vapor	656:660	arg1	barrier					662:668	water vapor barrier	650:668	water vapor barrier	650:668	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35468281	0	62	theme	Grape	84:88	arg1	Extract					95:101	Grape Seed Extract	84:101	Grape Seed Extract	84:101	Fabrication of Antioxidant and Antimicrobial Pullulan/Gelatin Films Integrated with Grape Seed Extract and Sulfur Nanoparticles.					
35468281	4	63	dep	significantly	595:607	arg1	<					612:612	p < 0.05	610:617	p < 0.05	610:617	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35468281	7	64	theme	packaging	1353:1361	arg1	applications					1363:1374	active food packaging applications	1341:1374	active food packaging applications	1341:1374	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	2	65	theme	translucent	366:376	arg1	film					392:395	a compatible but slightly translucent free-standing film	340:395	a compatible but slightly translucent free-standing film	340:395	A mixture of pullulan/gelatin (1:1) produced a compatible but slightly translucent free-standing film.					
35468281	7	66	theme	food	1348:1351	arg1	applications					1363:1374	active food packaging applications	1341:1374	active food packaging applications	1341:1374	Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.					
35468281	4	67	theme	GSE	591:593	arg1	addition					571:578	The addition	567:578	The addition of SNP and GSE	567:593	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35468281	5	68	theme	pullulan/gelatin-based	766:787	arg1	films					789:793	the pullulan/gelatin-based films	762:793	the pullulan/gelatin-based films	762:793	The mechanical performance of the pullulan/gelatin-based films was slightly decreased (∼10%), whereas the addition of fillers did not significantly affect the hydrophobicity and thermal stability.					
35468281	3	69	theme	functional	519:528	arg1	properties					494:503	the properties	490:503	the properties (physical and functional) of the pullulan/gelatin-based film	490:564	SNPs capped with enoki mushroom extract and GSE were added as functional fillers to improve the properties (physical and functional) of the pullulan/gelatin-based film.					
35468281	4	70	theme	p	610:610	arg1	<					612:612	p < 0.05	610:617	p < 0.05	610:617	The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films.					
35869684	4	0	theme	modified	551:558	arg1	technique					578:586	A modified ethanol injection technique	549:586	A modified ethanol injection technique	549:586	A modified ethanol injection technique was used for the fabrication of AGO bilosomes according to a D-optimal design.					
35869684	3	1	theme	topical	482:488	arg1	effect					472:477	the effect	468:477	the effect of topical AGO on intraocular pressure	468:516	This study aimed to formulate AGO into bilosomal vesicles for glaucoma treatment, as modern studies pointed out the effect of topical AGO on intraocular pressure for the treatment of glaucoma.					
35869684	6	2	theme	percent	836:842	arg1	responses					821:829	The measured responses	808:829	The measured responses	808:829	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	2	theme	percent	836:842	arg1	efficiency					855:864	percent entrapment efficiency	836:864	percent entrapment efficiency (EE%)	836:870	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	2	theme	percent	836:842	arg1	%					869:869	EE%	867:869	EE%	867:869	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	4	3	theme	injection	568:576	arg1	technique					578:586	A modified ethanol injection technique	549:586	A modified ethanol injection technique	549:586	A modified ethanol injection technique was used for the fabrication of AGO bilosomes according to a D-optimal design.					
35869684	10	4	theme	acceptable	1511:1520	arg1	stability					1522:1530	acceptable stability	1511:1530	acceptable stability	1511:1530	It exhibited acceptable stability over three months.					
35869684	7	5	theme	evaluation	1206:1215	arg1	studies					1217:1223	in vivo evaluation studies	1198:1223	in vivo evaluation studies	1198:1223	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	5	6	theme	independent	785:795	arg1	Phosphatidylcholine					667:685	Phosphatidylcholine	667:685	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type	667:766	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type were utilized as independent variables.					
35869684	5	6	theme	independent	785:795	arg1	variables					797:805	independent variables	785:805	independent variables	785:805	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type were utilized as independent variables.					
35869684	9	7	theme	spherical	1427:1435	arg1	shape					1437:1441	a typical spherical shape	1417:1441	a typical spherical shape of niosomes	1417:1453	It demonstrated a higher elasticity than their corresponding niosomes with a typical spherical shape of niosomes by using transmission electron microscope.					
35869684	7	8	theme	in	1198:1199	arg1	studies					1217:1223	in vivo evaluation studies	1198:1223	in vivo evaluation studies	1198:1223	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	11	9	theme	Refractive	1558:1567	arg1	index					1569:1573	Refractive index	1558:1573	Refractive index	1558:1573	pH and Refractive index measurements together with the histopathological study ensured that the OB formula is safe for the eye and causes no ocular irritation or blurred vision.					
35869684	11	10	theme	pH	1551:1552	arg1	measurements					1575:1586	pH and Refractive index measurements	1551:1586	pH and Refractive index measurements together with the histopathological study	1551:1628	pH and Refractive index measurements together with the histopathological study ensured that the OB formula is safe for the eye and causes no ocular irritation or blurred vision.					
35869684	11	11	theme	OB	1647:1648	arg1	safe					1661:1664	safe	1661:1664	safe	1661:1664	pH and Refractive index measurements together with the histopathological study ensured that the OB formula is safe for the eye and causes no ocular irritation or blurred vision.					
35869684	11	11	theme	OB	1647:1648	arg1	formula					1650:1656	the OB formula	1643:1656	the OB formula	1643:1656	pH and Refractive index measurements together with the histopathological study ensured that the OB formula is safe for the eye and causes no ocular irritation or blurred vision.					
35869684	6	12	theme	particle	873:880	arg1	size					882:885	particle size	873:885	particle size (PS)	873:890	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	12	theme	particle	873:880	arg1	PS					888:889	PS	888:889	PS	888:889	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	8	13	theme	OB	1230:1231	arg1	formula					1233:1239	The OB formula	1226:1239	The OB formula	1226:1239	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	7	14	theme	in	1167:1168	arg1	characterizations					1176:1192	further in vitro characterizations	1159:1192	further in vitro characterizations	1159:1192	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	0	15	theme	in	125:126	arg1	bilosomes					25:33	Hyaluronic acid-enriched bilosomes	0:33	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.	0:201	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	15	theme	in	125:126	arg1	characterization					134:149	in vitro characterization	125:149	in vitro characterization	125:149	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	9	16	theme	transmission	1464:1475	arg1	microscope					1486:1495	transmission electron microscope	1464:1495	transmission electron microscope	1464:1495	It demonstrated a higher elasticity than their corresponding niosomes with a typical spherical shape of niosomes by using transmission electron microscope.					
35869684	6	17	theme	EE	867:868	arg1	efficiency					855:864	percent entrapment efficiency	836:864	percent entrapment efficiency (EE%)	836:870	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	17	theme	EE	867:868	arg1	%					869:869	EE%	867:869	EE%	867:869	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	8	18	theme	%	1247:1247	arg1	%					1339:1339	75.14 ± 0.39%	1327:1339	75.14 ± 0.39%	1327:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	18	theme	%	1247:1247	arg1	432.45 ± 0.85 nm					1273:1288	432.45 ± 0.85 nm	1273:1288	432.45 ± 0.85 nm	1273:1288	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	18	theme	%	1247:1247	arg1	%					1311:1311	42.65 ± 0.52%	1299:1311	42.65 ± 0.52%	1299:1311	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	18	theme	%	1247:1247	arg1	%					1264:1264	EE% of 81.81 ± 0.23%	1245:1264	EE% of 81.81 ± 0.23%	1245:1264	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	0	19	theme	ocular	59:64	arg1	delivery					66:73	ocular delivery	59:73	ocular delivery of agomelatine	59:88	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	20	dep	in	125:126	arg1	vitro					128:132	vitro	128:132	vitro	128:132	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	5	21	theme	Hyaluronic	722:731	arg1	acid					733:736	Hyaluronic acid	722:736	Hyaluronic acid percentage (HA%)	722:753	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type were utilized as independent variables.					
35869684	6	22	theme	polydispersity	893:906	arg1	index					908:912	polydispersity index	893:912	polydispersity index	893:912	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	5	23	theme	activator	700:708	arg1	ratio					715:719	edge activator (EA) ratio	695:719	edge activator (EA) ratio	695:719	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type were utilized as independent variables.					
35869684	3	24	theme	glaucoma	418:425	arg1	treatment					427:435	glaucoma treatment	418:435	glaucoma treatment	418:435	This study aimed to formulate AGO into bilosomal vesicles for glaucoma treatment, as modern studies pointed out the effect of topical AGO on intraocular pressure for the treatment of glaucoma.					
35869684	4	25	theme	ethanol	560:566	arg1	technique					578:586	A modified ethanol injection technique	549:586	A modified ethanol injection technique	549:586	A modified ethanol injection technique was used for the fabrication of AGO bilosomes according to a D-optimal design.					
35869684	0	26	theme	Hyaluronic	0:9	arg1	bilosomes					25:33	Hyaluronic acid-enriched bilosomes	0:33	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.	0:201	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	26	theme	Hyaluronic	0:9	arg1	evaluation					180:189	in vivo pharmacodynamic evaluation	156:189	in vivo pharmacodynamic evaluation	156:189	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	26	theme	Hyaluronic	0:9	arg1	formulation					112:122	formulation	112:122	formulation	112:122	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	26	theme	Hyaluronic	0:9	arg1	characterization					134:149	in vitro characterization	125:149	in vitro characterization	125:149	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	7	27	theme	sodium	1120:1125	arg1	formula					1017:1023	The optimal bilosomal formula	995:1023	The optimal bilosomal formula (OB)	995:1028	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	27	theme	sodium	1120:1125	arg1	cholate					1127:1133	0.26% w/v HA and sodium cholate	1103:1133	cholate	1127:1133	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	0	28	from	evaluation	180:189	arg1	rabbits					194:200	rabbits	194:200	rabbits	194:200	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	3	29	theme	modern	441:446	arg1	studies					448:454	modern studies	441:454	modern studies	441:454	This study aimed to formulate AGO into bilosomal vesicles for glaucoma treatment, as modern studies pointed out the effect of topical AGO on intraocular pressure for the treatment of glaucoma.					
35869684	6	30	theme	drug	945:948	arg1	percentage					931:940	percentage	931:940	percentage of drug released after 2 h (Q2h%)	931:974	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	30	theme	drug	945:948	arg1	index					908:912	polydispersity index	893:912	polydispersity index	893:912	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	30	theme	drug	945:948	arg1	efficiency					855:864	percent entrapment efficiency	836:864	percent entrapment efficiency (EE%)	836:870	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	30	theme	drug	945:948	arg1	%					991:991	Q24h%	987:991	Q24h%	987:991	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	30	theme	drug	945:948	arg1	size					882:885	particle size	873:885	particle size (PS)	873:890	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	30	theme	drug	945:948	arg1	PS					888:889	PS	888:889	PS	888:889	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	30	theme	drug	945:948	arg1	responses					821:829	The measured responses	808:829	The measured responses	808:829	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	30	theme	drug	945:948	arg1	potential					920:928	zeta potential	915:928	zeta potential	915:928	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	30	theme	drug	945:948	arg1	%					869:869	EE%	867:869	EE%	867:869	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	30	theme	drug	945:948	arg1	24 h					981:984	24 h	981:984	24 h (Q24h%)	981:992	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	7	31	theme	optimal	999:1005	arg1	formula					1017:1023	The optimal bilosomal formula	995:1023	The optimal bilosomal formula (OB)	995:1028	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	31	theme	optimal	999:1005	arg1	HA					1113:1114	0.26% w/v HA and sodium cholate	1103:1133	HA	1113:1114	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	31	theme	optimal	999:1005	arg1	cholate					1127:1133	0.26% w/v HA and sodium cholate	1103:1133	cholate	1127:1133	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	31	theme	optimal	999:1005	arg1	OB					1026:1027	OB	1026:1027	OB	1026:1027	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	32	theme	%	1107:1107	arg1	formula					1017:1023	The optimal bilosomal formula	995:1023	The optimal bilosomal formula (OB)	995:1028	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	32	theme	%	1107:1107	arg1	HA					1113:1114	0.26% w/v HA and sodium cholate	1103:1133	HA	1113:1114	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	8	33	theme	Q24h	1318:1321	arg1	%					1322:1322	Q24h%	1318:1322	Q24h% of 75.14 ± 0.39%	1318:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	33	theme	Q24h	1318:1321	arg1	%					1339:1339	75.14 ± 0.39%	1327:1339	75.14 ± 0.39%	1327:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	33	theme	Q24h	1318:1321	arg1	432.45 ± 0.85 nm					1273:1288	432.45 ± 0.85 nm	1273:1288	432.45 ± 0.85 nm	1273:1288	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	33	theme	Q24h	1318:1321	arg1	%					1311:1311	42.65 ± 0.52%	1299:1311	42.65 ± 0.52%	1299:1311	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	12	34	from	superiority	1751:1761	arg1	parameters					1795:1804	the in vivo pharmacodynamics parameters	1766:1804	the in vivo pharmacodynamics parameters	1766:1804	The OB formula showed superiority in the in vivo pharmacodynamics parameters over the AGO solution, so AGO-loaded bilosome could improve ocular delivery and the bioavailability of agomelatine.					
35869684	6	35	theme	Q24h	987:990	arg1	24 h					981:984	24 h	981:984	24 h (Q24h%)	981:992	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	35	theme	Q24h	987:990	arg1	%					991:991	Q24h%	987:991	Q24h%	987:991	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	1	36	dep	Agomelatine	203:213	arg1	AGO					216:218	AGO	216:218	AGO	216:218	Agomelatine (AGO) is a dual-functional drug.					
35869684	11	37	theme	histopathological	1606:1622	arg1	study					1624:1628	the histopathological study	1602:1628	the histopathological study	1602:1628	pH and Refractive index measurements together with the histopathological study ensured that the OB formula is safe for the eye and causes no ocular irritation or blurred vision.					
35869684	9	38	theme	higher	1360:1365	arg1	elasticity					1367:1376	a higher elasticity	1358:1376	a higher elasticity than their corresponding niosomes with a typical spherical shape of niosomes	1358:1453	It demonstrated a higher elasticity than their corresponding niosomes with a typical spherical shape of niosomes by using transmission electron microscope.					
35869684	7	39	theme	EA	1093:1094	arg1	ratio					1096:1100	EA ratio	1093:1100	EA ratio	1093:1100	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	8	40	theme	%	1339:1339	arg1	%					1294:1294	Q2h%	1291:1294	Q2h% of 42.65 ± 0.52%	1291:1311	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	40	theme	%	1339:1339	arg1	%					1322:1322	Q24h%	1318:1322	Q24h% of 75.14 ± 0.39%	1318:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	40	theme	%	1339:1339	arg1	PS					1267:1268	PS	1267:1268	PS of 432.45 ± 0.85 nm	1267:1288	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	40	theme	%	1339:1339	arg1	432.45 ± 0.85 nm					1273:1288	432.45 ± 0.85 nm	1273:1288	432.45 ± 0.85 nm	1273:1288	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	40	theme	%	1339:1339	arg1	%					1311:1311	42.65 ± 0.52%	1299:1311	42.65 ± 0.52%	1299:1311	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	40	theme	%	1339:1339	arg1	%					1339:1339	75.14 ± 0.39%	1327:1339	75.14 ± 0.39%	1327:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	40	theme	%	1339:1339	arg1	%					1264:1264	EE% of 81.81 ± 0.23%	1245:1264	EE% of 81.81 ± 0.23%	1245:1264	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	9	41	with	niosomes	1403:1410	arg1	shape					1437:1441	a typical spherical shape	1417:1441	a typical spherical shape of niosomes	1417:1453	It demonstrated a higher elasticity than their corresponding niosomes with a typical spherical shape of niosomes by using transmission electron microscope.					
35869684	12	42	theme	in	1770:1771	arg1	parameters					1795:1804	the in vivo pharmacodynamics parameters	1766:1804	the in vivo pharmacodynamics parameters	1766:1804	The OB formula showed superiority in the in vivo pharmacodynamics parameters over the AGO solution, so AGO-loaded bilosome could improve ocular delivery and the bioavailability of agomelatine.					
35869684	7	43	theme	2:1 PC	1085:1090	arg1	0.814					1056:1060	0.814	1056:1060	0.814	1056:1060	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	43	theme	2:1 PC	1085:1090	arg1	composition					1070:1080	the composition	1066:1080	the composition of 2:1 PC	1066:1090	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	8	44	contain	had	1241:1243	arg2	432.45 ± 0.85 nm					1273:1288	432.45 ± 0.85 nm	1273:1288	432.45 ± 0.85 nm	1273:1288	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	44	contain	had	1241:1243	arg1	formula					1233:1239	The OB formula	1226:1239	The OB formula	1226:1239	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	44	contain	had	1241:1243	arg2	%					1311:1311	42.65 ± 0.52%	1299:1311	42.65 ± 0.52%	1299:1311	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	44	contain	had	1241:1243	arg2	%					1264:1264	EE% of 81.81 ± 0.23%	1245:1264	EE% of 81.81 ± 0.23%	1245:1264	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	44	contain	had	1241:1243	arg2	%					1339:1339	75.14 ± 0.39%	1327:1339	75.14 ± 0.39%	1327:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	44	contain	had	1241:1243	arg2	%					1322:1322	Q24h%	1318:1322	Q24h% of 75.14 ± 0.39%	1318:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	44	contain	had	1241:1243	arg2	%					1294:1294	Q2h%	1291:1294	Q2h% of 42.65 ± 0.52%	1291:1311	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	44	contain	had	1241:1243	arg2	PS					1267:1268	PS	1267:1268	PS of 432.45 ± 0.85 nm	1267:1288	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	0	45	from	formulation	112:122	arg1	rabbits					194:200	rabbits	194:200	rabbits	194:200	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	46	theme	pharmacodynamic	164:178	arg1	evaluation					180:189	in vivo pharmacodynamic evaluation	156:189	in vivo pharmacodynamic evaluation	156:189	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	46	theme	pharmacodynamic	164:178	arg1	bilosomes					25:33	Hyaluronic acid-enriched bilosomes	0:33	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.	0:201	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	1	47	theme	dual-functional	226:240	arg1	drug					242:245	a dual-functional drug	224:245	a dual-functional drug	224:245	Agomelatine (AGO) is a dual-functional drug.					
35869684	1	47	theme	dual-functional	226:240	arg1	Agomelatine					203:213	Agomelatine	203:213	Agomelatine (AGO)	203:219	Agomelatine (AGO) is a dual-functional drug.					
35869684	12	48	theme	pharmacodynamics	1778:1793	arg1	parameters					1795:1804	the in vivo pharmacodynamics parameters	1766:1804	the in vivo pharmacodynamics parameters	1766:1804	The OB formula showed superiority in the in vivo pharmacodynamics parameters over the AGO solution, so AGO-loaded bilosome could improve ocular delivery and the bioavailability of agomelatine.					
35869684	6	49	theme	entrapment	844:853	arg1	responses					821:829	The measured responses	808:829	The measured responses	808:829	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	49	theme	entrapment	844:853	arg1	efficiency					855:864	percent entrapment efficiency	836:864	percent entrapment efficiency (EE%)	836:870	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	49	theme	entrapment	844:853	arg1	%					869:869	EE%	867:869	EE%	867:869	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	9	50	theme	corresponding	1389:1401	arg1	niosomes					1403:1410	their corresponding niosomes	1383:1410	their corresponding niosomes with a typical spherical shape of niosomes	1383:1453	It demonstrated a higher elasticity than their corresponding niosomes with a typical spherical shape of niosomes by using transmission electron microscope.					
35869684	7	51	theme	composition	1070:1080	arg1	desirability					1040:1051	the desirability	1036:1051	the desirability of 0.814 and the composition of 2:1 PC	1036:1090	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	5	52	used	utilized	773:780	arg2	PC					688:689	PC	688:689	PC	688:689	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type were utilized as independent variables.					
35869684	5	52	used	utilized	773:780	arg2	variables					797:805	independent variables	785:805	independent variables	785:805	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type were utilized as independent variables.					
35869684	5	52	used	utilized	773:780	arg2	Phosphatidylcholine					667:685	Phosphatidylcholine	667:685	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type	667:766	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type were utilized as independent variables.					
35869684	0	53	from	characterization	134:149	arg1	rabbits					194:200	rabbits	194:200	rabbits	194:200	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	3	54	theme	intraocular	497:507	arg1	pressure					509:516	intraocular pressure	497:516	intraocular pressure	497:516	This study aimed to formulate AGO into bilosomal vesicles for glaucoma treatment, as modern studies pointed out the effect of topical AGO on intraocular pressure for the treatment of glaucoma.					
35869684	6	55	theme	measured	812:819	arg1	responses					821:829	The measured responses	808:829	The measured responses	808:829	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	55	theme	measured	812:819	arg1	efficiency					855:864	percent entrapment efficiency	836:864	percent entrapment efficiency (EE%)	836:870	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	9	56	theme	typical	1419:1425	arg1	shape					1437:1441	a typical spherical shape	1417:1441	a typical spherical shape of niosomes	1417:1453	It demonstrated a higher elasticity than their corresponding niosomes with a typical spherical shape of niosomes by using transmission electron microscope.					
35869684	4	57	theme	bilosomes	624:632	arg1	fabrication					605:615	the fabrication	601:615	the fabrication of AGO bilosomes according to a D-optimal design	601:664	A modified ethanol injection technique was used for the fabrication of AGO bilosomes according to a D-optimal design.					
35869684	3	58	theme	glaucoma	539:546	arg1	treatment					526:534	the treatment	522:534	the treatment of glaucoma	522:546	This study aimed to formulate AGO into bilosomal vesicles for glaucoma treatment, as modern studies pointed out the effect of topical AGO on intraocular pressure for the treatment of glaucoma.					
35869684	7	59	dep	in	1167:1168	arg1	vitro					1170:1174	vitro	1170:1174	vitro	1170:1174	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	60	with	formula	1017:1023	arg1	desirability					1040:1051	the desirability	1036:1051	the desirability of 0.814 and the composition of 2:1 PC	1036:1090	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	11	61	theme	index	1569:1573	arg1	measurements					1575:1586	pH and Refractive index measurements	1551:1586	pH and Refractive index measurements together with the histopathological study	1551:1628	pH and Refractive index measurements together with the histopathological study ensured that the OB formula is safe for the eye and causes no ocular irritation or blurred vision.					
35869684	4	62	used	used	592:595	arg2	technique					578:586	A modified ethanol injection technique	549:586	A modified ethanol injection technique	549:586	A modified ethanol injection technique was used for the fabrication of AGO bilosomes according to a D-optimal design.					
35869684	3	63	from	effect	472:477	arg1	pressure					509:516	intraocular pressure	497:516	intraocular pressure	497:516	This study aimed to formulate AGO into bilosomal vesicles for glaucoma treatment, as modern studies pointed out the effect of topical AGO on intraocular pressure for the treatment of glaucoma.					
35869684	5	64	theme	EA	760:761	arg1	type					763:766	EA type	760:766	EA type	760:766	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type were utilized as independent variables.					
35869684	12	65	theme	OB	1733:1734	arg1	formula					1736:1742	The OB formula	1729:1742	The OB formula	1729:1742	The OB formula showed superiority in the in vivo pharmacodynamics parameters over the AGO solution, so AGO-loaded bilosome could improve ocular delivery and the bioavailability of agomelatine.					
35869684	9	66	theme	niosomes	1446:1453	arg1	shape					1437:1441	a typical spherical shape	1417:1441	a typical spherical shape of niosomes	1417:1453	It demonstrated a higher elasticity than their corresponding niosomes with a typical spherical shape of niosomes by using transmission electron microscope.					
35869684	11	67	theme	blurred	1713:1719	arg1	vision					1721:1726	blurred vision	1713:1726	blurred vision	1713:1726	pH and Refractive index measurements together with the histopathological study ensured that the OB formula is safe for the eye and causes no ocular irritation or blurred vision.					
35869684	8	68	theme	EE	1245:1246	arg1	%					1339:1339	75.14 ± 0.39%	1327:1339	75.14 ± 0.39%	1327:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	68	theme	EE	1245:1246	arg1	432.45 ± 0.85 nm					1273:1288	432.45 ± 0.85 nm	1273:1288	432.45 ± 0.85 nm	1273:1288	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	68	theme	EE	1245:1246	arg1	%					1311:1311	42.65 ± 0.52%	1299:1311	42.65 ± 0.52%	1299:1311	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	68	theme	EE	1245:1246	arg1	%					1264:1264	EE% of 81.81 ± 0.23%	1245:1264	EE% of 81.81 ± 0.23%	1245:1264	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	12	69	theme	ocular	1866:1871	arg1	delivery					1873:1880	ocular delivery	1866:1880	ocular delivery	1866:1880	The OB formula showed superiority in the in vivo pharmacodynamics parameters over the AGO solution, so AGO-loaded bilosome could improve ocular delivery and the bioavailability of agomelatine.					
35869684	5	70	theme	edge	695:698	arg1	ratio					715:719	edge activator (EA) ratio	695:719	edge activator (EA) ratio	695:719	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type were utilized as independent variables.					
35869684	0	71	dep	in	156:157	arg1	vivo					159:162	vivo	159:162	vivo	159:162	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	9	72	theme	electron	1477:1484	arg1	microscope					1486:1495	transmission electron microscope	1464:1495	transmission electron microscope	1464:1495	It demonstrated a higher elasticity than their corresponding niosomes with a typical spherical shape of niosomes by using transmission electron microscope.					
35869684	8	73	theme	of	1249:1250	arg1	%					1339:1339	75.14 ± 0.39%	1327:1339	75.14 ± 0.39%	1327:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	73	theme	of	1249:1250	arg1	432.45 ± 0.85 nm					1273:1288	432.45 ± 0.85 nm	1273:1288	432.45 ± 0.85 nm	1273:1288	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	73	theme	of	1249:1250	arg1	%					1311:1311	42.65 ± 0.52%	1299:1311	42.65 ± 0.52%	1299:1311	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	73	theme	of	1249:1250	arg1	%					1264:1264	EE% of 81.81 ± 0.23%	1245:1264	EE% of 81.81 ± 0.23%	1245:1264	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	7	74	theme	further	1159:1165	arg1	characterizations					1176:1192	further in vitro characterizations	1159:1192	further in vitro characterizations	1159:1192	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	0	75	theme	in	156:157	arg1	evaluation					180:189	in vivo pharmacodynamic evaluation	156:189	in vivo pharmacodynamic evaluation	156:189	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	75	theme	in	156:157	arg1	bilosomes					25:33	Hyaluronic acid-enriched bilosomes	0:33	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.	0:201	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	12	76	theme	AGO-loaded	1832:1841	arg1	bilosome					1843:1850	AGO-loaded bilosome	1832:1850	AGO-loaded bilosome	1832:1850	The OB formula showed superiority in the in vivo pharmacodynamics parameters over the AGO solution, so AGO-loaded bilosome could improve ocular delivery and the bioavailability of agomelatine.					
35869684	7	77	theme	0.814	1056:1060	arg1	desirability					1040:1051	the desirability	1036:1051	the desirability of 0.814 and the composition of 2:1 PC	1036:1090	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	6	78	theme	zeta	915:918	arg1	potential					920:928	zeta potential	915:928	zeta potential	915:928	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	4	79	theme	D-optimal	649:657	arg1	design					659:664	a D-optimal design	647:664	a D-optimal design	647:664	A modified ethanol injection technique was used for the fabrication of AGO bilosomes according to a D-optimal design.					
35869684	0	80	theme	agomelatine	78:88	arg1	delivery					66:73	ocular delivery	59:73	ocular delivery of agomelatine	59:88	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	7	81	dep	in	1198:1199	arg1	vivo					1201:1204	vivo	1201:1204	vivo	1201:1204	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	8	82	theme	432.45 ± 0.85 nm	1273:1288	arg1	%					1294:1294	Q2h%	1291:1294	Q2h% of 42.65 ± 0.52%	1291:1311	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	82	theme	432.45 ± 0.85 nm	1273:1288	arg1	%					1322:1322	Q24h%	1318:1322	Q24h% of 75.14 ± 0.39%	1318:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	82	theme	432.45 ± 0.85 nm	1273:1288	arg1	PS					1267:1268	PS	1267:1268	PS of 432.45 ± 0.85 nm	1267:1288	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	82	theme	432.45 ± 0.85 nm	1273:1288	arg1	432.45 ± 0.85 nm					1273:1288	432.45 ± 0.85 nm	1273:1288	432.45 ± 0.85 nm	1273:1288	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	82	theme	432.45 ± 0.85 nm	1273:1288	arg1	%					1311:1311	42.65 ± 0.52%	1299:1311	42.65 ± 0.52%	1299:1311	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	82	theme	432.45 ± 0.85 nm	1273:1288	arg1	%					1339:1339	75.14 ± 0.39%	1327:1339	75.14 ± 0.39%	1327:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	82	theme	432.45 ± 0.85 nm	1273:1288	arg1	%					1264:1264	EE% of 81.81 ± 0.23%	1245:1264	EE% of 81.81 ± 0.23%	1245:1264	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	7	83	dep	formula	1017:1023	arg1	ratio					1096:1100	EA ratio	1093:1100	EA ratio	1093:1100	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	0	84	theme	D-optimal	94:102	arg1	design					104:109	D-optimal design	94:109	D-optimal design	94:109	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	85	dep	bilosomes	25:33	arg1	evaluation					180:189	in vivo pharmacodynamic evaluation	156:189	in vivo pharmacodynamic evaluation	156:189	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	85	dep	bilosomes	25:33	arg1	approach					39:46	an approach	36:46	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.	0:201	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	85	dep	bilosomes	25:33	arg1	formulation					112:122	formulation	112:122	formulation	112:122	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	85	dep	bilosomes	25:33	arg1	bilosomes					25:33	Hyaluronic acid-enriched bilosomes	0:33	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.	0:201	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	85	dep	bilosomes	25:33	arg1	characterization					134:149	in vitro characterization	125:149	in vitro characterization	125:149	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	6	86	theme	Q2h	970:972	arg1	2 h					965:967	2 h	965:967	2 h (Q2h%)	965:974	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	6	86	theme	Q2h	970:972	arg1	%					973:973	Q2h%	970:973	Q2h%	970:973	The measured responses were percent entrapment efficiency (EE%), particle size (PS), polydispersity index, zeta potential, percentage of drug released after 2 h (Q2h%), and 24 h (Q24h%).					
35869684	0	87	theme	acid-enriched	11:23	arg1	bilosomes					25:33	Hyaluronic acid-enriched bilosomes	0:33	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.	0:201	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	87	theme	acid-enriched	11:23	arg1	evaluation					180:189	in vivo pharmacodynamic evaluation	156:189	in vivo pharmacodynamic evaluation	156:189	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	87	theme	acid-enriched	11:23	arg1	formulation					112:122	formulation	112:122	formulation	112:122	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	0	87	theme	acid-enriched	11:23	arg1	characterization					134:149	in vitro characterization	125:149	in vitro characterization	125:149	Hyaluronic acid-enriched bilosomes: an approach to enhance ocular delivery of agomelatine via D-optimal design: formulation, in vitro characterization, and in vivo pharmacodynamic evaluation in rabbits.					
35869684	8	88	theme	%	1311:1311	arg1	%					1294:1294	Q2h%	1291:1294	Q2h% of 42.65 ± 0.52%	1291:1311	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	88	theme	%	1311:1311	arg1	%					1322:1322	Q24h%	1318:1322	Q24h% of 75.14 ± 0.39%	1318:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	88	theme	%	1311:1311	arg1	PS					1267:1268	PS	1267:1268	PS of 432.45 ± 0.85 nm	1267:1288	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	88	theme	%	1311:1311	arg1	432.45 ± 0.85 nm					1273:1288	432.45 ± 0.85 nm	1273:1288	432.45 ± 0.85 nm	1273:1288	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	88	theme	%	1311:1311	arg1	%					1311:1311	42.65 ± 0.52%	1299:1311	42.65 ± 0.52%	1299:1311	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	88	theme	%	1311:1311	arg1	%					1339:1339	75.14 ± 0.39%	1327:1339	75.14 ± 0.39%	1327:1339	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	8	88	theme	%	1311:1311	arg1	%					1264:1264	EE% of 81.81 ± 0.23%	1245:1264	EE% of 81.81 ± 0.23%	1245:1264	The OB formula had EE% of 81.81 ± 0.23%, PS of 432.45 ± 0.85 nm, Q2h% of 42.65 ± 0.52%, and Q24h% of 75.14 ± 0.39%.					
35869684	7	89	theme	bilosomal	1007:1015	arg1	formula					1017:1023	The optimal bilosomal formula	995:1023	The optimal bilosomal formula (OB)	995:1028	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	89	theme	bilosomal	1007:1015	arg1	HA					1113:1114	0.26% w/v HA and sodium cholate	1103:1133	HA	1113:1114	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	89	theme	bilosomal	1007:1015	arg1	cholate					1127:1133	0.26% w/v HA and sodium cholate	1103:1133	cholate	1127:1133	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	89	theme	bilosomal	1007:1015	arg1	OB					1026:1027	OB	1026:1027	OB	1026:1027	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	90	theme	w/v	1109:1111	arg1	formula					1017:1023	The optimal bilosomal formula	995:1023	The optimal bilosomal formula (OB)	995:1028	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	7	90	theme	w/v	1109:1111	arg1	HA					1113:1114	0.26% w/v HA and sodium cholate	1103:1133	HA	1113:1114	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	11	91	theme	ocular	1692:1697	arg1	irritation					1699:1708	no ocular irritation	1689:1708	no ocular irritation	1689:1708	pH and Refractive index measurements together with the histopathological study ensured that the OB formula is safe for the eye and causes no ocular irritation or blurred vision.					
35869684	5	92	theme	acid	733:736	arg1	percentage					738:747	Hyaluronic acid percentage	722:747	Hyaluronic acid percentage (HA%)	722:753	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type were utilized as independent variables.					
35869684	5	92	theme	acid	733:736	arg1	%					752:752	HA%	750:752	HA%	750:752	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type were utilized as independent variables.					
35869684	7	93	theme	0.26	1103:1106	arg1	%					1107:1107	%	1107:1107	%	1107:1107	The optimal bilosomal formula (OB), with the desirability of 0.814 and the composition of 2:1 PC: EA ratio, 0.26% w/v HA and sodium cholate as EA, was subjected to further in vitro characterizations and in vivo evaluation studies.					
35869684	3	94	theme	bilosomal	395:403	arg1	vesicles					405:412	bilosomal vesicles	395:412	bilosomal vesicles for glaucoma treatment	395:435	This study aimed to formulate AGO into bilosomal vesicles for glaucoma treatment, as modern studies pointed out the effect of topical AGO on intraocular pressure for the treatment of glaucoma.					
35869684	5	95	theme	EA	711:712	arg1	ratio					715:719	edge activator (EA) ratio	695:719	edge activator (EA) ratio	695:719	Phosphatidylcholine (PC) to edge activator (EA) ratio, Hyaluronic acid percentage (HA%), and EA type were utilized as independent variables.					
35869684	12	96	dep	in	1770:1771	arg1	vivo					1773:1776	vivo	1773:1776	vivo	1773:1776	The OB formula showed superiority in the in vivo pharmacodynamics parameters over the AGO solution, so AGO-loaded bilosome could improve ocular delivery and the bioavailability of agomelatine.					
35869684	12	97	theme	agomelatine	1909:1919	arg1	delivery					1873:1880	ocular delivery	1866:1880	ocular delivery	1866:1880	The OB formula showed superiority in the in vivo pharmacodynamics parameters over the AGO solution, so AGO-loaded bilosome could improve ocular delivery and the bioavailability of agomelatine.					
35869684	12	97	theme	agomelatine	1909:1919	arg1	bioavailability					1890:1904	the bioavailability	1886:1904	the bioavailability of agomelatine	1886:1919	The OB formula showed superiority in the in vivo pharmacodynamics parameters over the AGO solution, so AGO-loaded bilosome could improve ocular delivery and the bioavailability of agomelatine.					
36693105	7	0	theme	maximal	1326:1332	arg1	speed					1343:1347	maximal swimming speed	1326:1347	maximal swimming speed	1326:1347	Ex vivo and in vivo analysis confirmed that these investment strategies come with a functional trade-off, decreasing cavefish muscle fiber shortening velocity, time to maximal force, and ultimately maximal swimming speed.					
36693105	7	1	theme	functional	1212:1221	arg1	trade-off					1223:1231	a functional trade-off	1210:1231	a functional trade-off	1210:1231	Ex vivo and in vivo analysis confirmed that these investment strategies come with a functional trade-off, decreasing cavefish muscle fiber shortening velocity, time to maximal force, and ultimately maximal swimming speed.					
36693105	2	2	theme	inactivity	372:381	arg1	effect					344:349	the effect	340:349	the effect of adaptive physical inactivity on tissue homeostasis	340:403	Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis.					
36693105	2	3	from	effect	344:349	arg1	homeostasis					393:403	tissue homeostasis	386:403	tissue homeostasis	386:403	Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis.					
36693105	3	4	theme	river-dwelling	588:601	arg1	counterpart					603:613	their river-dwelling counterpart	582:613	their river-dwelling counterpart	582:613	One such example, the Mexican cavefish Astyanax mexicanus, has lost moderate-to-vigorous activity following cave colonization, reaching basal swim speeds ~3.7-fold slower than their river-dwelling counterpart.					
36693105	9	5	theme	metabolic	1530:1538	arg1	phosphorylation					1568:1582	phosphorylation	1568:1582	phosphorylation of Pgm1-Threonine 19	1568:1603	Multi-omic analysis suggested metabolic reprogramming, specifically phosphorylation of Pgm1-Threonine 19, as a key component enhancing cavefish glycogen metabolism and sustained muscle contraction.					
36693105	9	5	theme	metabolic	1530:1538	arg1	reprogramming					1540:1552	metabolic reprogramming	1530:1552	metabolic reprogramming	1530:1552	Multi-omic analysis suggested metabolic reprogramming, specifically phosphorylation of Pgm1-Threonine 19, as a key component enhancing cavefish glycogen metabolism and sustained muscle contraction.					
36693105	9	5	theme	metabolic	1530:1538	arg1	component					1615:1623	a key component	1609:1623	a key component enhancing cavefish glycogen metabolism and sustained muscle contraction	1609:1695	Multi-omic analysis suggested metabolic reprogramming, specifically phosphorylation of Pgm1-Threonine 19, as a key component enhancing cavefish glycogen metabolism and sustained muscle contraction.					
36693105	4	6	theme	total	705:709	arg1	mass					718:721	total muscle mass	705:721	total muscle mass	705:721	This change in behavior is accompanied by a marked shift in body composition, decreasing total muscle mass and increasing fat mass.					
36693105	6	7	theme	wild-caught	933:943	arg1	cavefish					945:952	laboratory-reared and wild-caught cavefish	911:952	laboratory-reared and wild-caught cavefish	911:952	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	0	8	theme	muscle	78:83	arg1	mass					85:88	muscle mass	78:88	muscle mass	78:88	Metabolic reprogramming underlies cavefish muscular endurance despite loss of muscle mass and contractility.					
36693105	10	9	theme	skeletal	1728:1735	arg1	changes					1744:1750	broad skeletal muscle changes	1722:1750	broad skeletal muscle changes	1722:1750	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	10	10	theme	muscle	1947:1952	arg1	contraction					1954:1964	sustained muscle contraction	1937:1964	sustained muscle contraction	1937:1964	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	1	11	theme	muscle	191:196	arg1	wasting					198:204	muscle wasting	191:204	muscle wasting	191:204	Physical inactivity is a scourge to human health, promoting metabolic disease and muscle wasting.					
36693105	6	12	theme	regulator	1034:1042	arg1	expression					1003:1012	increased expression	993:1012	increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression	993:1125	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	4	13	theme	body	676:679	arg1	composition					681:691	body composition	676:691	body composition	676:691	This change in behavior is accompanied by a marked shift in body composition, decreasing total muscle mass and increasing fat mass.					
36693105	2	14	theme	evolutionary	310:321	arg1	perspective					323:333	an evolutionary perspective	307:333	an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis	307:403	Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis.					
36693105	9	15	theme	Pgm1-Threonine	1587:1600	arg1	reprogramming					1540:1552	metabolic reprogramming	1530:1552	metabolic reprogramming	1530:1552	Multi-omic analysis suggested metabolic reprogramming, specifically phosphorylation of Pgm1-Threonine 19, as a key component enhancing cavefish glycogen metabolism and sustained muscle contraction.					
36693105	9	15	theme	Pgm1-Threonine	1587:1600	arg1	phosphorylation					1568:1582	phosphorylation	1568:1582	phosphorylation of Pgm1-Threonine 19	1568:1603	Multi-omic analysis suggested metabolic reprogramming, specifically phosphorylation of Pgm1-Threonine 19, as a key component enhancing cavefish glycogen metabolism and sustained muscle contraction.					
36693105	6	16	theme	pparγ-the	1017:1025	arg1	regulator					1034:1042	pparγ-the master regulator	1017:1042	pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression	1017:1125	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	10	17	theme	cave	1762:1765	arg1	colonization					1767:1778	cave colonization	1762:1778	cave colonization	1762:1778	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	7	18	theme	in vivo	1140:1146	arg1	analysis					1148:1155	Ex vivo and in vivo analysis	1128:1155	analysis	1148:1155	Ex vivo and in vivo analysis confirmed that these investment strategies come with a functional trade-off, decreasing cavefish muscle fiber shortening velocity, time to maximal force, and ultimately maximal swimming speed.					
36693105	7	19	theme	muscle	1254:1259	arg1	velocity					1278:1285	cavefish muscle fiber shortening velocity	1245:1285	cavefish muscle fiber shortening velocity	1245:1285	Ex vivo and in vivo analysis confirmed that these investment strategies come with a functional trade-off, decreasing cavefish muscle fiber shortening velocity, time to maximal force, and ultimately maximal swimming speed.					
36693105	7	20	theme	Ex vivo	1128:1134	arg1	analysis					1148:1155	Ex vivo and in vivo analysis	1128:1155	analysis	1148:1155	Ex vivo and in vivo analysis confirmed that these investment strategies come with a functional trade-off, decreasing cavefish muscle fiber shortening velocity, time to maximal force, and ultimately maximal swimming speed.					
36693105	2	21	theme	multiple	222:229	arg1	niches					242:247	multiple ecological niches	222:247	multiple ecological niches	222:247	Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis.					
36693105	6	22	theme	myosin	1097:1102	arg1	chain					1110:1114	fast myosin heavy chain	1092:1114	fast myosin heavy chain expression	1092:1125	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	6	23	dep	regulator	1034:1042	arg1	decrease					1080:1087	a simultaneous decrease	1065:1087	pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression	1017:1125	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	8	24	theme	muscular	1404:1411	arg1	endurance					1413:1421	muscular endurance	1404:1421	muscular endurance	1404:1421	Despite this, cavefish displayed a striking degree of muscular endurance, reaching maximal swim speeds ~3.5-fold faster than their basal swim speeds.					
36693105	2	25	theme	relaxed	254:260	arg1	investment					262:271	relaxed investment	254:271	relaxed investment into physical activity	254:294	Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis.					
36693105	9	26	theme	cavefish	1635:1642	arg1	metabolism					1653:1662	cavefish glycogen metabolism	1635:1662	cavefish glycogen metabolism	1635:1662	Multi-omic analysis suggested metabolic reprogramming, specifically phosphorylation of Pgm1-Threonine 19, as a key component enhancing cavefish glycogen metabolism and sustained muscle contraction.					
36693105	0	27	theme	mass	85:88	arg1	loss					70:73	loss	70:73	loss of muscle mass and contractility	70:106	Metabolic reprogramming underlies cavefish muscular endurance despite loss of muscle mass and contractility.					
36693105	1	28	theme	promoting	159:167	arg1	disease					179:185	promoting metabolic disease	159:185	promoting metabolic disease	159:185	Physical inactivity is a scourge to human health, promoting metabolic disease and muscle wasting.					
36693105	6	29	theme	simultaneous	1067:1078	arg1	decrease					1080:1087	a simultaneous decrease	1065:1087	pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression	1017:1125	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	10	30	theme	high-fat	1866:1873	arg1	models					1875:1880	mammalian disuse and high-fat models	1845:1880	models	1875:1880	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	8	31	theme	maximal	1433:1439	arg1	speeds					1446:1451	maximal swim speeds	1433:1451	maximal swim speeds ~3.5-fold faster than their basal swim speeds	1433:1497	Despite this, cavefish displayed a striking degree of muscular endurance, reaching maximal swim speeds ~3.5-fold faster than their basal swim speeds.					
36693105	0	32	theme	contractility	94:106	arg1	loss					70:73	loss	70:73	loss of muscle mass and contractility	70:106	Metabolic reprogramming underlies cavefish muscular endurance despite loss of muscle mass and contractility.					
36693105	5	33	from	expense	852:858	arg1	lipid					816:820	increased lipid and sugar accumulation	806:843	lipid	816:820	This shift persisted at the single muscle fiber level via increased lipid and sugar accumulation at the expense of myofibrillar volume.					
36693105	5	33	from	expense	852:858	arg1	accumulation					832:843	increased lipid and sugar accumulation	806:843	accumulation	832:843	This shift persisted at the single muscle fiber level via increased lipid and sugar accumulation at the expense of myofibrillar volume.					
36693105	10	34	theme	unique	1917:1922	arg1	capacity					1924:1931	a unique capacity	1915:1931	a unique capacity for sustained muscle contraction	1915:1964	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	9	35	theme	sustained	1668:1676	arg1	contraction					1685:1695	sustained muscle contraction	1668:1695	sustained muscle contraction	1668:1695	Multi-omic analysis suggested metabolic reprogramming, specifically phosphorylation of Pgm1-Threonine 19, as a key component enhancing cavefish glycogen metabolism and sustained muscle contraction.					
36693105	6	36	theme	adipogenesis-with	1047:1063	arg1	regulator					1034:1042	pparγ-the master regulator	1017:1042	pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression	1017:1125	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	10	37	theme	adaptive	1795:1802	arg1	phenotype					1820:1828	an adaptive skeletal muscle phenotype	1792:1828	an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism	1792:1997	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	5	38	theme	muscle	783:788	arg1	level					796:800	the single muscle fiber level	772:800	the single muscle fiber level	772:800	This shift persisted at the single muscle fiber level via increased lipid and sugar accumulation at the expense of myofibrillar volume.					
36693105	6	39	from	decrease	1080:1087	arg1	expression					1116:1125	fast myosin heavy chain expression	1092:1125	fast myosin heavy chain expression	1092:1125	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	5	40	theme	fiber	790:794	arg1	level					796:800	the single muscle fiber level	772:800	the single muscle fiber level	772:800	This shift persisted at the single muscle fiber level via increased lipid and sugar accumulation at the expense of myofibrillar volume.					
36693105	8	41	theme	faster	1463:1468	arg1	speeds					1446:1451	maximal swim speeds	1433:1451	maximal swim speeds ~3.5-fold faster than their basal swim speeds	1433:1497	Despite this, cavefish displayed a striking degree of muscular endurance, reaching maximal swim speeds ~3.5-fold faster than their basal swim speeds.					
36693105	0	42	theme	Metabolic	0:8	arg1	reprogramming					10:22	Metabolic reprogramming	0:22	Metabolic reprogramming	0:22	Metabolic reprogramming underlies cavefish muscular endurance despite loss of muscle mass and contractility.					
36693105	10	43	theme	reminiscent	1830:1840	arg1	phenotype					1820:1828	an adaptive skeletal muscle phenotype	1792:1828	an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism	1792:1997	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	7	44	theme	fiber	1261:1265	arg1	velocity					1278:1285	cavefish muscle fiber shortening velocity	1245:1285	cavefish muscle fiber shortening velocity	1245:1285	Ex vivo and in vivo analysis confirmed that these investment strategies come with a functional trade-off, decreasing cavefish muscle fiber shortening velocity, time to maximal force, and ultimately maximal swimming speed.					
36693105	8	45	theme	swim	1487:1490	arg1	speeds					1492:1497	their basal swim speeds	1475:1497	their basal swim speeds	1475:1497	Despite this, cavefish displayed a striking degree of muscular endurance, reaching maximal swim speeds ~3.5-fold faster than their basal swim speeds.					
36693105	10	46	theme	muscle	1813:1818	arg1	phenotype					1820:1828	an adaptive skeletal muscle phenotype	1792:1828	an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism	1792:1997	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	0	47	theme	muscular	43:50	arg1	endurance					52:60	cavefish muscular endurance	34:60	cavefish muscular endurance	34:60	Metabolic reprogramming underlies cavefish muscular endurance despite loss of muscle mass and contractility.					
36693105	7	48	theme	cavefish	1245:1252	arg1	velocity					1278:1285	cavefish muscle fiber shortening velocity	1245:1285	cavefish muscle fiber shortening velocity	1245:1285	Ex vivo and in vivo analysis confirmed that these investment strategies come with a functional trade-off, decreasing cavefish muscle fiber shortening velocity, time to maximal force, and ultimately maximal swimming speed.					
36693105	6	49	theme	chain	1110:1114	arg1	expression					1116:1125	fast myosin heavy chain expression	1092:1125	fast myosin heavy chain expression	1092:1125	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	2	50	theme	physical	363:370	arg1	inactivity					372:381	adaptive physical inactivity	354:381	adaptive physical inactivity	354:381	Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis.					
36693105	6	51	theme	Transcriptomic	884:897	arg1	analysis					899:906	Transcriptomic analysis	884:906	Transcriptomic analysis of laboratory-reared and wild-caught cavefish	884:952	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	3	52	theme	cave	514:517	arg1	colonization					519:530	cave colonization	514:530	cave colonization	514:530	One such example, the Mexican cavefish Astyanax mexicanus, has lost moderate-to-vigorous activity following cave colonization, reaching basal swim speeds ~3.7-fold slower than their river-dwelling counterpart.					
36693105	10	53	theme	skeletal	1804:1811	arg1	phenotype					1820:1828	an adaptive skeletal muscle phenotype	1792:1828	an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism	1792:1997	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	4	54	theme	marked	660:665	arg1	shift					667:671	a marked shift	658:671	a marked shift in body composition	658:691	This change in behavior is accompanied by a marked shift in body composition, decreasing total muscle mass and increasing fat mass.					
36693105	3	55	theme	basal	542:546	arg1	speeds					553:558	basal swim speeds	542:558	basal swim speeds ~3.7-fold slower than their river-dwelling counterpart	542:613	One such example, the Mexican cavefish Astyanax mexicanus, has lost moderate-to-vigorous activity following cave colonization, reaching basal swim speeds ~3.7-fold slower than their river-dwelling counterpart.					
36693105	9	56	theme	Multi-omic	1500:1509	arg1	analysis					1511:1518	Multi-omic analysis	1500:1518	Multi-omic analysis	1500:1518	Multi-omic analysis suggested metabolic reprogramming, specifically phosphorylation of Pgm1-Threonine 19, as a key component enhancing cavefish glycogen metabolism and sustained muscle contraction.					
36693105	7	57	theme	swimming	1334:1341	arg1	speed					1343:1347	maximal swimming speed	1326:1347	maximal swimming speed	1326:1347	Ex vivo and in vivo analysis confirmed that these investment strategies come with a functional trade-off, decreasing cavefish muscle fiber shortening velocity, time to maximal force, and ultimately maximal swimming speed.					
36693105	7	58	theme	investment	1178:1187	arg1	strategies					1189:1198	these investment strategies	1172:1198	these investment strategies	1172:1198	Ex vivo and in vivo analysis confirmed that these investment strategies come with a functional trade-off, decreasing cavefish muscle fiber shortening velocity, time to maximal force, and ultimately maximal swimming speed.					
36693105	3	59	theme	slower	570:575	arg1	speeds					553:558	basal swim speeds	542:558	basal swim speeds ~3.7-fold slower than their river-dwelling counterpart	542:613	One such example, the Mexican cavefish Astyanax mexicanus, has lost moderate-to-vigorous activity following cave colonization, reaching basal swim speeds ~3.7-fold slower than their river-dwelling counterpart.					
36693105	1	60	theme	metabolic	169:177	arg1	disease					179:185	promoting metabolic disease	159:185	promoting metabolic disease	159:185	Physical inactivity is a scourge to human health, promoting metabolic disease and muscle wasting.					
36693105	6	61	theme	cavefish	945:952	arg1	analysis					899:906	Transcriptomic analysis	884:906	Transcriptomic analysis of laboratory-reared and wild-caught cavefish	884:952	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	10	62	theme	muscle	1737:1742	arg1	changes					1744:1750	broad skeletal muscle changes	1722:1750	broad skeletal muscle changes	1722:1750	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	5	63	theme	increased	806:814	arg1	lipid					816:820	increased lipid and sugar accumulation	806:843	lipid	816:820	This shift persisted at the single muscle fiber level via increased lipid and sugar accumulation at the expense of myofibrillar volume.					
36693105	10	64	theme	enhanced	1970:1977	arg1	metabolism					1988:1997	enhanced glycogen metabolism	1970:1997	enhanced glycogen metabolism	1970:1997	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	4	65	theme	muscle	711:716	arg1	mass					718:721	total muscle mass	705:721	total muscle mass	705:721	This change in behavior is accompanied by a marked shift in body composition, decreasing total muscle mass and increasing fat mass.					
36693105	10	66	theme	broad	1722:1726	arg1	changes					1744:1750	broad skeletal muscle changes	1722:1750	broad skeletal muscle changes	1722:1750	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	10	67	theme	sustained	1937:1945	arg1	contraction					1954:1964	sustained muscle contraction	1937:1964	sustained muscle contraction	1937:1964	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	5	68	theme	myofibrillar	863:874	arg1	volume					876:881	myofibrillar volume	863:881	myofibrillar volume	863:881	This shift persisted at the single muscle fiber level via increased lipid and sugar accumulation at the expense of myofibrillar volume.					
36693105	6	69	theme	master	1027:1032	arg1	regulator					1034:1042	pparγ-the master regulator	1017:1042	pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression	1017:1125	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	4	70	theme	fat	738:740	arg1	mass					742:745	fat mass	738:745	fat mass	738:745	This change in behavior is accompanied by a marked shift in body composition, decreasing total muscle mass and increasing fat mass.					
36693105	2	71	theme	ecological	231:240	arg1	niches					242:247	multiple ecological niches	222:247	multiple ecological niches	222:247	Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis.					
36693105	6	72	theme	increased	993:1001	arg1	expression					1003:1012	increased expression	993:1012	increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression	993:1125	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	2	73	theme	physical	278:285	arg1	activity					287:294	physical activity	278:294	physical activity	278:294	Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis.					
36693105	9	74	theme	key	1611:1613	arg1	reprogramming					1540:1552	metabolic reprogramming	1530:1552	metabolic reprogramming	1530:1552	Multi-omic analysis suggested metabolic reprogramming, specifically phosphorylation of Pgm1-Threonine 19, as a key component enhancing cavefish glycogen metabolism and sustained muscle contraction.					
36693105	9	74	theme	key	1611:1613	arg1	component					1615:1623	a key component	1609:1623	a key component enhancing cavefish glycogen metabolism and sustained muscle contraction	1609:1695	Multi-omic analysis suggested metabolic reprogramming, specifically phosphorylation of Pgm1-Threonine 19, as a key component enhancing cavefish glycogen metabolism and sustained muscle contraction.					
36693105	6	75	theme	heavy	1104:1108	arg1	chain					1110:1114	fast myosin heavy chain	1092:1114	fast myosin heavy chain expression	1092:1125	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	8	76	theme	striking	1385:1392	arg1	degree					1394:1399	a striking degree	1383:1399	a striking degree of muscular endurance	1383:1421	Despite this, cavefish displayed a striking degree of muscular endurance, reaching maximal swim speeds ~3.5-fold faster than their basal swim speeds.					
36693105	10	77	theme	mammalian	1845:1853	arg1	disuse					1855:1860	mammalian disuse and high-fat models	1845:1880	disuse	1855:1860	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	4	78	from	shift	667:671	arg1	composition					681:691	body composition	676:691	body composition	676:691	This change in behavior is accompanied by a marked shift in body composition, decreasing total muscle mass and increasing fat mass.					
36693105	1	79	theme	human	145:149	arg1	health					151:156	human health	145:156	human health	145:156	Physical inactivity is a scourge to human health, promoting metabolic disease and muscle wasting.					
36693105	6	80	theme	fast	1092:1095	arg1	chain					1110:1114	fast myosin heavy chain	1092:1114	fast myosin heavy chain expression	1092:1125	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	7	81	theme	maximal	1296:1302	arg1	force					1304:1308	maximal force	1296:1308	maximal force	1296:1308	Ex vivo and in vivo analysis confirmed that these investment strategies come with a functional trade-off, decreasing cavefish muscle fiber shortening velocity, time to maximal force, and ultimately maximal swimming speed.					
36693105	9	82	theme	glycogen	1644:1651	arg1	metabolism					1653:1662	cavefish glycogen metabolism	1635:1662	cavefish glycogen metabolism	1635:1662	Multi-omic analysis suggested metabolic reprogramming, specifically phosphorylation of Pgm1-Threonine 19, as a key component enhancing cavefish glycogen metabolism and sustained muscle contraction.					
36693105	8	83	theme	endurance	1413:1421	arg1	degree					1394:1399	a striking degree	1383:1399	a striking degree of muscular endurance	1383:1421	Despite this, cavefish displayed a striking degree of muscular endurance, reaching maximal swim speeds ~3.5-fold faster than their basal swim speeds.					
36693105	5	84	theme	single	776:781	arg1	level					796:800	the single muscle fiber level	772:800	the single muscle fiber level	772:800	This shift persisted at the single muscle fiber level via increased lipid and sugar accumulation at the expense of myofibrillar volume.					
36693105	5	85	theme	volume	876:881	arg1	expense					852:858	the expense	848:858	the expense of myofibrillar volume	848:881	This shift persisted at the single muscle fiber level via increased lipid and sugar accumulation at the expense of myofibrillar volume.					
36693105	9	86	theme	muscle	1678:1683	arg1	contraction					1685:1695	sustained muscle contraction	1668:1695	sustained muscle contraction	1668:1695	Multi-omic analysis suggested metabolic reprogramming, specifically phosphorylation of Pgm1-Threonine 19, as a key component enhancing cavefish glycogen metabolism and sustained muscle contraction.					
36693105	8	87	theme	swim	1441:1444	arg1	speeds					1446:1451	maximal swim speeds	1433:1451	maximal swim speeds ~3.5-fold faster than their basal swim speeds	1433:1497	Despite this, cavefish displayed a striking degree of muscular endurance, reaching maximal swim speeds ~3.5-fold faster than their basal swim speeds.					
36693105	4	88	from	change	621:626	arg1	behavior					631:638	behavior	631:638	behavior	631:638	This change in behavior is accompanied by a marked shift in body composition, decreasing total muscle mass and increasing fat mass.					
36693105	0	89	theme	cavefish	34:41	arg1	endurance					52:60	cavefish muscular endurance	34:60	cavefish muscular endurance	34:60	Metabolic reprogramming underlies cavefish muscular endurance despite loss of muscle mass and contractility.					
36693105	1	90	theme	Physical	109:116	arg1	scourge					134:140	a scourge	132:140	a scourge to human health, promoting metabolic disease and muscle wasting	132:204	Physical inactivity is a scourge to human health, promoting metabolic disease and muscle wasting.					
36693105	1	90	theme	Physical	109:116	arg1	inactivity					118:127	Physical inactivity	109:127	Physical inactivity	109:127	Physical inactivity is a scourge to human health, promoting metabolic disease and muscle wasting.					
36693105	10	91	dep	disuse	1855:1860	arg1	maintaining					1903:1913	maintaining	1903:1913	maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism	1903:1997	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	6	92	theme	laboratory-reared	911:927	arg1	cavefish					945:952	laboratory-reared and wild-caught cavefish	911:952	laboratory-reared and wild-caught cavefish	911:952	Transcriptomic analysis of laboratory-reared and wild-caught cavefish indicated that this shift is driven by increased expression of pparγ-the master regulator of adipogenesis-with a simultaneous decrease in fast myosin heavy chain expression.					
36693105	8	93	theme	basal	1481:1485	arg1	speeds					1492:1497	their basal swim speeds	1475:1497	their basal swim speeds	1475:1497	Despite this, cavefish displayed a striking degree of muscular endurance, reaching maximal swim speeds ~3.5-fold faster than their basal swim speeds.					
36693105	3	94	theme	moderate-to-vigorous	474:493	arg1	activity					495:502	moderate-to-vigorous activity	474:502	moderate-to-vigorous activity	474:502	One such example, the Mexican cavefish Astyanax mexicanus, has lost moderate-to-vigorous activity following cave colonization, reaching basal swim speeds ~3.7-fold slower than their river-dwelling counterpart.					
36693105	5	95	theme	sugar	826:830	arg1	accumulation					832:843	increased lipid and sugar accumulation	806:843	accumulation	832:843	This shift persisted at the single muscle fiber level via increased lipid and sugar accumulation at the expense of myofibrillar volume.					
36693105	3	96	theme	swim	548:551	arg1	speeds					553:558	basal swim speeds	542:558	basal swim speeds ~3.7-fold slower than their river-dwelling counterpart	542:613	One such example, the Mexican cavefish Astyanax mexicanus, has lost moderate-to-vigorous activity following cave colonization, reaching basal swim speeds ~3.7-fold slower than their river-dwelling counterpart.					
36693105	2	97	theme	adaptive	354:361	arg1	inactivity					372:381	adaptive physical inactivity	354:381	adaptive physical inactivity	354:381	Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis.					
36693105	10	98	theme	glycogen	1979:1986	arg1	metabolism					1988:1997	enhanced glycogen metabolism	1970:1997	enhanced glycogen metabolism	1970:1997	Collectively, we reveal broad skeletal muscle changes following cave colonization, displaying an adaptive skeletal muscle phenotype reminiscent to mammalian disuse and high-fat models while simultaneously maintaining a unique capacity for sustained muscle contraction via enhanced glycogen metabolism.					
36693105	2	99	theme	tissue	386:391	arg1	homeostasis					393:403	tissue homeostasis	386:403	tissue homeostasis	386:403	Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis.					
36693105	7	100	theme	shortening	1267:1276	arg1	velocity					1278:1285	cavefish muscle fiber shortening velocity	1245:1285	cavefish muscle fiber shortening velocity	1245:1285	Ex vivo and in vivo analysis confirmed that these investment strategies come with a functional trade-off, decreasing cavefish muscle fiber shortening velocity, time to maximal force, and ultimately maximal swimming speed.					
36693105	2	101	contain	have	249:252	arg1	niches					242:247	multiple ecological niches	222:247	multiple ecological niches	222:247	Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis.					
36693105	2	101	contain	have	249:252	arg2	investment					262:271	relaxed investment	254:271	relaxed investment into physical activity	254:294	Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis.					
35127940	4	0	theme	renal	805:809	arg1	ECM					811:813	Porcine renal ECM	797:813	METHODS Porcine renal ECM	789:813	METHODS Porcine renal ECM was prepared by decellularization using 1% Triton X-100, solubilized with 0.5 M acetic acid.					
35127940	1	1	theme	kidney-related	236:249	arg1	experiments					251:261	kidney-related experiments	236:261	kidney-related experiments in vitro/ex vivo	236:278	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	6	2	theme	gene	1111:1114	arg1	expressions					1116:1126	renal-associated gene expressions	1094:1126	renal-associated gene expressions	1094:1126	The efficacies as coating, hydrogel, and scaffold materials were analyzed through cell morphology, proliferation rate, renal-associated gene expressions, chemical composition, and microstructure evaluation.					
35127940	14	3	theme	amide	2106:2110	arg1	expression					2198:2207	renal-associated gene expression	2176:2207	renal-associated gene expression	2176:2207	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	3	theme	amide	2106:2110	arg1	peaks					2112:2116	specific amide peaks	2097:2116	specific amide peaks	2097:2116	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	3	theme	amide	2106:2110	arg1	rate					2166:2169	cell proliferation rate	2147:2169	cell proliferation rate	2147:2169	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	3	theme	amide	2106:2110	arg1	material					2070:2077	the scaffold material	2057:2077	the scaffold material	2057:2077	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	3	theme	amide	2106:2110	arg1	porosity					2137:2144	enhanced internal porosity	2119:2144	enhanced internal porosity	2119:2144	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	3	4	theme	cell	500:503	arg1	usage					505:509	RPTE cell usage	495:509	RPTE cell usage	495:509	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	1	5	theme	tubular	179:185	arg1	tool					227:230	a very useful tool	213:230	a very useful tool for kidney-related experiments in vitro/ex vivo	213:278	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	1	5	theme	tubular	179:185	arg1	cell					205:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	16	6	theme	renal	2369:2373	arg1	ECM					2375:2377	the coated renal ECM	2358:2377	the coated renal ECM	2358:2377	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	6	7	theme	proliferation	1074:1086	arg1	rate					1088:1091	proliferation rate	1074:1091	proliferation rate	1074:1091	The efficacies as coating, hydrogel, and scaffold materials were analyzed through cell morphology, proliferation rate, renal-associated gene expressions, chemical composition, and microstructure evaluation.					
35127940	14	8	theme	renal	2080:2084	arg1	ECM					2086:2088	renal ECM	2080:2088	renal ECM	2080:2088	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	11	9	theme	renal	1709:1713	arg1	ECM					1715:1717	the renal ECM	1705:1717	the renal ECM than other materials	1705:1738	Renal-associated gene expressions were significantly enhanced in the renal ECM than other materials.					
35127940	16	10	theme	culture	2476:2482	arg1	niche					2484:2488	useful 3D culture niche and cell delivery vehicles	2466:2515	niche	2484:2488	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	4	11	theme	%	856:856	arg1	X-100					865:869	1% Triton X-100	855:869	1% Triton X-100	855:869	METHODS Porcine renal ECM was prepared by decellularization using 1% Triton X-100, solubilized with 0.5 M acetic acid.					
35127940	0	12	theme	Proximal	113:120	arg1	Cell					141:144	Renal Proximal Tubular Epithelial Cell	107:144	Renal Proximal Tubular Epithelial Cell	107:144	Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell.					
35127940	16	13	dep	hydrogel	2423:2430	arg1	the					2419:2421	the	2419:2421	the	2419:2421	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	10	14	theme	coating	1522:1528	arg1	ECM					1546:1548	renal ECM	1540:1548	renal ECM	1540:1548	RESULTS As the coating material, renal ECM showed a higher cell proliferation rate compared to other materials, except for Matrigel.					
35127940	10	14	theme	coating	1522:1528	arg1	material					1530:1537	the coating material	1518:1537	the coating material	1518:1537	RESULTS As the coating material, renal ECM showed a higher cell proliferation rate compared to other materials, except for Matrigel.					
35127940	0	15	theme	Epithelial	130:139	arg1	Cell					141:144	Renal Proximal Tubular Epithelial Cell	107:144	Renal Proximal Tubular Epithelial Cell	107:144	Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell.					
35127940	13	16	theme	HA	2041:2042	arg1	hydrogel					2044:2051	HA hydrogel	2041:2051	HA hydrogel	2041:2051	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	3	17	theme	porcine	538:544	arg1	matrix					575:580	porcine kidney-derived extracellular matrix	538:580	porcine kidney-derived extracellular matrix (renal ECM)	538:592	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	3	17	theme	porcine	538:544	arg1	ECM					589:591	renal ECM	583:591	renal ECM	583:591	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	16	18	theme	delivery	2499:2506	arg1	vehicles					2508:2515	useful 3D culture niche and cell delivery vehicles	2466:2515	vehicles	2508:2515	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	8	19	theme	concentration	1360:1372	arg1	range					1374:1378	the effective concentration range	1346:1378	the effective concentration range	1346:1378	After confirmation of coating effects, the effective concentration range was decided.					
35127940	3	20	theme	three-dimensional	712:728	arg1	niche					735:739	three-dimensional (3D) niche	712:739	three-dimensional (3D) niche	712:739	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	3	20	theme	three-dimensional	712:728	arg1	vehicle					780:786	a valuable cell delivery vehicle	755:786	a valuable cell delivery vehicle	755:786	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	13	21	theme	renal-associated	1994:2009	arg1	expressions					2016:2026	renal-associated gene expressions	1994:2026	renal-associated gene expressions	1994:2026	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	15	22	theme	renal	2270:2274	arg1	material					2298:2305	a suitable material	2287:2305	a suitable material for RPTE cell culture and usage	2287:2337	CONCLUSIONS We concluded that renal ECM can be a suitable material for RPTE cell culture and usage.					
35127940	15	22	theme	renal	2270:2274	arg1	ECM					2276:2278	renal ECM	2270:2278	renal ECM	2270:2278	CONCLUSIONS We concluded that renal ECM can be a suitable material for RPTE cell culture and usage.					
35127940	11	23	theme	Renal-associated	1640:1655	arg1	expressions					1662:1672	Renal-associated gene expressions	1640:1672	Renal-associated gene expressions	1640:1672	Renal-associated gene expressions were significantly enhanced in the renal ECM than other materials.					
35127940	3	24	theme	delivery	771:778	arg1	niche					735:739	three-dimensional (3D) niche	712:739	three-dimensional (3D) niche	712:739	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	3	24	theme	delivery	771:778	arg1	vehicle					780:786	a valuable cell delivery vehicle	755:786	a valuable cell delivery vehicle	755:786	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	2	25	theme	cell	393:396	arg1	low					406:408	low	406:408	low	406:408	However, only a few primary RPTE cells can be obtained through kidney biopsy, the proliferation rate of primary cell is very low, and the cultured cell properties are easily altered in artificial conditions.					
35127940	2	25	theme	cell	393:396	arg1	rate					377:380	the proliferation rate	359:380	the proliferation rate of primary cell	359:396	However, only a few primary RPTE cells can be obtained through kidney biopsy, the proliferation rate of primary cell is very low, and the cultured cell properties are easily altered in artificial conditions.					
35127940	14	26	theme	col1	2225:2228	arg1	scaffold					2230:2237	the col1 scaffold	2221:2237	the col1 scaffold	2221:2237	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	3	27	theme	extracellular	561:573	arg1	matrix					575:580	porcine kidney-derived extracellular matrix	538:580	porcine kidney-derived extracellular matrix (renal ECM)	538:592	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	3	27	theme	extracellular	561:573	arg1	ECM					589:591	renal ECM	583:591	renal ECM	583:591	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	1	28	theme	BACKGROUND	147:156	arg1	tool					227:230	a very useful tool	213:230	a very useful tool for kidney-related experiments in vitro/ex vivo	213:278	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	1	28	theme	BACKGROUND	147:156	arg1	cell					205:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	3	29	theme	valuable	757:764	arg1	niche					735:739	three-dimensional (3D) niche	712:739	three-dimensional (3D) niche	712:739	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	3	29	theme	valuable	757:764	arg1	vehicle					780:786	a valuable cell delivery vehicle	755:786	a valuable cell delivery vehicle	755:786	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	15	30	theme	cell	2316:2319	arg1	culture					2321:2327	RPTE cell culture	2311:2327	RPTE cell culture	2311:2327	CONCLUSIONS We concluded that renal ECM can be a suitable material for RPTE cell culture and usage.					
35127940	1	31	theme	renal	164:168	arg1	tool					227:230	a very useful tool	213:230	a very useful tool for kidney-related experiments in vitro/ex vivo	213:278	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	1	31	theme	renal	164:168	arg1	cell					205:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	16	32	theme	ECM	2454:2456	arg1	scaffold					2436:2443	scaffold	2436:2443	scaffold	2436:2443	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	16	32	theme	ECM	2454:2456	arg1	hydrogel					2423:2430	hydrogel	2423:2430	hydrogel	2423:2430	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	9	33	dep	hydrogel	1411:1418	arg1	materials					1433:1441	materials	1433:1441	materials	1433:1441	The efficacies as hydrogel and scaffold materials were compared with hyaluronic acid (HA) and col1, respectively.					
35127940	0	34	theme	Scaffold	85:92	arg1	Material					94:101	Scaffold Material	85:101	Scaffold Material	85:101	Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell.					
35127940	3	35	dep	three-dimensional	712:728	arg1	3D					731:732	3D	731:732	3D	731:732	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	9	36	theme	hyaluronic	1462:1471	arg1	acid					1473:1476	hyaluronic acid	1462:1476	hyaluronic acid (HA)	1462:1481	The efficacies as hydrogel and scaffold materials were compared with hyaluronic acid (HA) and col1, respectively.					
35127940	9	36	theme	hyaluronic	1462:1471	arg1	HA					1479:1480	HA	1479:1480	HA	1479:1480	The efficacies as hydrogel and scaffold materials were compared with hyaluronic acid (HA) and col1, respectively.					
35127940	16	37	theme	useful	2466:2471	arg1	niche					2484:2488	useful 3D culture niche and cell delivery vehicles	2466:2515	niche	2484:2488	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	4	38	theme	0.5 M	889:893	arg1	acid					902:905	0.5 M acetic acid	889:905	0.5 M acetic acid	889:905	METHODS Porcine renal ECM was prepared by decellularization using 1% Triton X-100, solubilized with 0.5 M acetic acid.					
35127940	2	39	theme	primary	385:391	arg1	cell					393:396	primary cell	385:396	primary cell	385:396	However, only a few primary RPTE cells can be obtained through kidney biopsy, the proliferation rate of primary cell is very low, and the cultured cell properties are easily altered in artificial conditions.					
35127940	14	40	theme	renal-associated	2176:2191	arg1	expression					2198:2207	renal-associated gene expression	2176:2207	renal-associated gene expression	2176:2207	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	40	theme	renal-associated	2176:2191	arg1	peaks					2112:2116	specific amide peaks	2097:2116	specific amide peaks	2097:2116	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	40	theme	renal-associated	2176:2191	arg1	rate					2166:2169	cell proliferation rate	2147:2169	cell proliferation rate	2147:2169	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	40	theme	renal-associated	2176:2191	arg1	material					2070:2077	the scaffold material	2057:2077	the scaffold material	2057:2077	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	40	theme	renal-associated	2176:2191	arg1	porosity					2137:2144	enhanced internal porosity	2119:2144	enhanced internal porosity	2119:2144	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	2	41	theme	proliferation	363:375	arg1	low					406:408	low	406:408	low	406:408	However, only a few primary RPTE cells can be obtained through kidney biopsy, the proliferation rate of primary cell is very low, and the cultured cell properties are easily altered in artificial conditions.					
35127940	2	41	theme	proliferation	363:375	arg1	rate					377:380	the proliferation rate	359:380	the proliferation rate of primary cell	359:396	However, only a few primary RPTE cells can be obtained through kidney biopsy, the proliferation rate of primary cell is very low, and the cultured cell properties are easily altered in artificial conditions.					
35127940	13	42	theme	cell	1942:1945	arg1	material					1898:1905	the hydrogel material	1885:1905	the hydrogel material	1885:1905	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	13	42	theme	cell	1942:1945	arg1	morphology					1955:1964	a distinct inner cell network morphology	1925:1964	a distinct inner cell network morphology	1925:1964	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	3	43	link	kidney-derived	546:559	arg1	matrix					575:580	porcine kidney-derived extracellular matrix	538:580	porcine kidney-derived extracellular matrix (renal ECM)	538:592	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	3	43	link	kidney-derived	546:559	arg1	ECM					589:591	renal ECM	583:591	renal ECM	583:591	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	12	44	dep	100 μg	1874:1879	arg1	to					1871:1872	to	1871:1872	to	1871:1872	Coating effect on cell proliferation was dependent on the renal ECM concentration, and the effective concentration ranged from 30 to 100 μg.					
35127940	10	45	theme	renal	1540:1544	arg1	ECM					1546:1548	renal ECM	1540:1548	renal ECM	1540:1548	RESULTS As the coating material, renal ECM showed a higher cell proliferation rate compared to other materials, except for Matrigel.					
35127940	10	45	theme	renal	1540:1544	arg1	material					1530:1537	the coating material	1518:1537	the coating material	1518:1537	RESULTS As the coating material, renal ECM showed a higher cell proliferation rate compared to other materials, except for Matrigel.					
35127940	12	46	theme	ECM	1805:1807	arg1	concentration					1809:1821	the renal ECM concentration	1795:1821	the renal ECM concentration	1795:1821	Coating effect on cell proliferation was dependent on the renal ECM concentration, and the effective concentration ranged from 30 to 100 μg.					
35127940	14	47	theme	proliferation	2152:2164	arg1	peaks					2112:2116	specific amide peaks	2097:2116	specific amide peaks	2097:2116	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	47	theme	proliferation	2152:2164	arg1	expression					2198:2207	renal-associated gene expression	2176:2207	renal-associated gene expression	2176:2207	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	47	theme	proliferation	2152:2164	arg1	rate					2166:2169	cell proliferation rate	2147:2169	cell proliferation rate	2147:2169	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	47	theme	proliferation	2152:2164	arg1	material					2070:2077	the scaffold material	2057:2077	the scaffold material	2057:2077	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	47	theme	proliferation	2152:2164	arg1	porosity					2137:2144	enhanced internal porosity	2119:2144	enhanced internal porosity	2119:2144	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	10	48	theme	proliferation	1571:1583	arg1	rate					1585:1588	a higher cell proliferation rate	1557:1588	a higher cell proliferation rate	1557:1588	RESULTS As the coating material, renal ECM showed a higher cell proliferation rate compared to other materials, except for Matrigel.					
35127940	0	49	theme	Porcine	15:21	arg1	Matrix					52:57	Porcine Kidney-Derived Extracellular Matrix	15:57	Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material	15:101	Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell.					
35127940	16	50	theme	RPTE	2390:2393	arg1	proliferation					2400:2412	RPTE cell proliferation	2390:2412	RPTE cell proliferation	2390:2412	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	2	51	theme	cell	428:431	arg1	properties					433:442	the cultured cell properties	415:442	the cultured cell properties	415:442	However, only a few primary RPTE cells can be obtained through kidney biopsy, the proliferation rate of primary cell is very low, and the cultured cell properties are easily altered in artificial conditions.					
35127940	10	52	theme	higher	1559:1564	arg1	rate					1585:1588	a higher cell proliferation rate	1557:1588	a higher cell proliferation rate	1557:1588	RESULTS As the coating material, renal ECM showed a higher cell proliferation rate compared to other materials, except for Matrigel.					
35127940	0	53	theme	Extracellular	38:50	arg1	Matrix					52:57	Porcine Kidney-Derived Extracellular Matrix	15:57	Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material	15:101	Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell.					
35127940	14	54	dep	showed	2090:2095	arg1	compared					2209:2216	compared	2209:2216	showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold	2090:2237	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	10	55	theme	other	1602:1606	arg1	materials					1608:1616	other materials	1602:1616	other materials	1602:1616	RESULTS As the coating material, renal ECM showed a higher cell proliferation rate compared to other materials, except for Matrigel.					
35127940	12	56	theme	Coating	1741:1747	arg1	effect					1749:1754	Coating effect	1741:1754	Coating effect on cell proliferation	1741:1776	Coating effect on cell proliferation was dependent on the renal ECM concentration, and the effective concentration ranged from 30 to 100 μg.					
35127940	16	57	theme	cell	2535:2538	arg1	properties					2540:2549	renal cell properties	2529:2549	renal cell properties	2529:2549	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	8	58	theme	coating	1329:1335	arg1	effects					1337:1343	coating effects	1329:1343	coating effects	1329:1343	After confirmation of coating effects, the effective concentration range was decided.					
35127940	2	59	theme	RPTE	309:312	arg1	cells					314:318	only a few primary RPTE cells	290:318	only a few primary RPTE cells	290:318	However, only a few primary RPTE cells can be obtained through kidney biopsy, the proliferation rate of primary cell is very low, and the cultured cell properties are easily altered in artificial conditions.					
35127940	5	60	theme	protein	918:924	arg1	concentration					926:938	The final protein concentration	908:938	The final protein concentration	908:938	The final protein concentration was adjusted to 10 μg/μL (pH 7.0).					
35127940	7	61	theme	coating	1202:1208	arg1	material					1210:1217	a coating material	1200:1217	a coating material	1200:1217	The efficacies as a coating material were compared with Matrigel, collagen type 1 (col1), gelatin, fibrinogen, and thrombin.					
35127940	13	62	theme	hydrogel	1889:1896	arg1	material					1898:1905	the hydrogel material	1885:1905	the hydrogel material	1885:1905	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	13	62	theme	hydrogel	1889:1896	arg1	morphology					1955:1964	a distinct inner cell network morphology	1925:1964	a distinct inner cell network morphology	1925:1964	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	2	63	theme	kidney	344:349	arg1	biopsy					351:356	kidney biopsy	344:356	kidney biopsy	344:356	However, only a few primary RPTE cells can be obtained through kidney biopsy, the proliferation rate of primary cell is very low, and the cultured cell properties are easily altered in artificial conditions.					
35127940	1	64	theme	useful	220:225	arg1	tool					227:230	a very useful tool	213:230	a very useful tool for kidney-related experiments in vitro/ex vivo	213:278	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	1	64	theme	useful	220:225	arg1	cell					205:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	14	65	theme	enhanced	2119:2126	arg1	expression					2198:2207	renal-associated gene expression	2176:2207	renal-associated gene expression	2176:2207	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	65	theme	enhanced	2119:2126	arg1	peaks					2112:2116	specific amide peaks	2097:2116	specific amide peaks	2097:2116	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	65	theme	enhanced	2119:2126	arg1	rate					2166:2169	cell proliferation rate	2147:2169	cell proliferation rate	2147:2169	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	65	theme	enhanced	2119:2126	arg1	material					2070:2077	the scaffold material	2057:2077	the scaffold material	2057:2077	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	65	theme	enhanced	2119:2126	arg1	porosity					2137:2144	enhanced internal porosity	2119:2144	enhanced internal porosity	2119:2144	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	4	66	theme	Porcine	797:803	arg1	ECM					811:813	Porcine renal ECM	797:813	METHODS Porcine renal ECM	789:813	METHODS Porcine renal ECM was prepared by decellularization using 1% Triton X-100, solubilized with 0.5 M acetic acid.					
35127940	16	67	theme	cell	2395:2398	arg1	proliferation					2400:2412	RPTE cell proliferation	2390:2412	RPTE cell proliferation	2390:2412	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	3	68	theme	scaffold	620:627	arg1	material					629:636	scaffold material	620:636	scaffold material	620:636	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	3	69	theme	RPTE	495:498	arg1	usage					505:509	RPTE cell usage	495:509	RPTE cell usage	495:509	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	16	70	theme	coated	2362:2367	arg1	ECM					2375:2377	the coated renal ECM	2358:2377	the coated renal ECM	2358:2377	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	6	71	theme	renal-associated	1094:1109	arg1	expressions					1116:1126	renal-associated gene expressions	1094:1126	renal-associated gene expressions	1094:1126	The efficacies as coating, hydrogel, and scaffold materials were analyzed through cell morphology, proliferation rate, renal-associated gene expressions, chemical composition, and microstructure evaluation.					
35127940	14	72	theme	specific	2097:2104	arg1	expression					2198:2207	renal-associated gene expression	2176:2207	renal-associated gene expression	2176:2207	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	72	theme	specific	2097:2104	arg1	peaks					2112:2116	specific amide peaks	2097:2116	specific amide peaks	2097:2116	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	72	theme	specific	2097:2104	arg1	rate					2166:2169	cell proliferation rate	2147:2169	cell proliferation rate	2147:2169	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	72	theme	specific	2097:2104	arg1	material					2070:2077	the scaffold material	2057:2077	the scaffold material	2057:2077	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	72	theme	specific	2097:2104	arg1	porosity					2137:2144	enhanced internal porosity	2119:2144	enhanced internal porosity	2119:2144	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	1	73	theme	proximal	170:177	arg1	tool					227:230	a very useful tool	213:230	a very useful tool for kidney-related experiments in vitro/ex vivo	213:278	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	1	73	theme	proximal	170:177	arg1	cell					205:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	3	74	theme	cell	650:653	arg1	proliferation					655:667	cell proliferation	650:667	cell proliferation	650:667	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	1	75	theme	epithelial	187:196	arg1	tool					227:230	a very useful tool	213:230	a very useful tool for kidney-related experiments in vitro/ex vivo	213:278	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	1	75	theme	epithelial	187:196	arg1	cell					205:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	0	76	theme	Tubular	122:128	arg1	Cell					141:144	Renal Proximal Tubular Epithelial Cell	107:144	Renal Proximal Tubular Epithelial Cell	107:144	Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell.					
35127940	1	77	theme	RPTE	199:202	arg1	tool					227:230	a very useful tool	213:230	a very useful tool for kidney-related experiments in vitro/ex vivo	213:278	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	1	77	theme	RPTE	199:202	arg1	cell					205:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	16	78	theme	cell	2494:2497	arg1	vehicles					2508:2515	useful 3D culture niche and cell delivery vehicles	2466:2515	vehicles	2508:2515	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	4	79	theme	1	855:855	arg1	%					856:856	%	856:856	%	856:856	METHODS Porcine renal ECM was prepared by decellularization using 1% Triton X-100, solubilized with 0.5 M acetic acid.					
35127940	8	80	theme	effective	1350:1358	arg1	range					1374:1378	the effective concentration range	1346:1378	the effective concentration range	1346:1378	After confirmation of coating effects, the effective concentration range was decided.					
35127940	6	81	theme	cell	1057:1060	arg1	morphology					1062:1071	cell morphology	1057:1071	cell morphology	1057:1071	The efficacies as coating, hydrogel, and scaffold materials were analyzed through cell morphology, proliferation rate, renal-associated gene expressions, chemical composition, and microstructure evaluation.					
35127940	14	82	theme	scaffold	2061:2068	arg1	expression					2198:2207	renal-associated gene expression	2176:2207	renal-associated gene expression	2176:2207	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	82	theme	scaffold	2061:2068	arg1	peaks					2112:2116	specific amide peaks	2097:2116	specific amide peaks	2097:2116	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	82	theme	scaffold	2061:2068	arg1	rate					2166:2169	cell proliferation rate	2147:2169	cell proliferation rate	2147:2169	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	82	theme	scaffold	2061:2068	arg1	material					2070:2077	the scaffold material	2057:2077	the scaffold material	2057:2077	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	82	theme	scaffold	2061:2068	arg1	porosity					2137:2144	enhanced internal porosity	2119:2144	enhanced internal porosity	2119:2144	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	3	83	theme	cellular	682:689	arg1	properties					691:700	cellular properties	682:700	cellular properties	682:700	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	4	84	theme	Triton	858:863	arg1	X-100					865:869	1% Triton X-100	855:869	1% Triton X-100	855:869	METHODS Porcine renal ECM was prepared by decellularization using 1% Triton X-100, solubilized with 0.5 M acetic acid.					
35127940	6	85	theme	microstructure	1155:1168	arg1	evaluation					1170:1179	microstructure evaluation	1155:1179	microstructure evaluation	1155:1179	The efficacies as coating, hydrogel, and scaffold materials were analyzed through cell morphology, proliferation rate, renal-associated gene expressions, chemical composition, and microstructure evaluation.					
35127940	12	86	from	effect	1749:1754	arg1	proliferation					1764:1776	cell proliferation	1759:1776	cell proliferation	1759:1776	Coating effect on cell proliferation was dependent on the renal ECM concentration, and the effective concentration ranged from 30 to 100 μg.					
35127940	1	87	from	experiments	251:261	arg1	vitro/ex					266:273	vitro/ex	266:273	vitro/ex vivo	266:278	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	11	88	theme	other	1724:1728	arg1	materials					1730:1738	other materials	1724:1738	other materials	1724:1738	Renal-associated gene expressions were significantly enhanced in the renal ECM than other materials.					
35127940	13	89	theme	gene	2011:2014	arg1	expressions					2016:2026	renal-associated gene expressions	1994:2026	renal-associated gene expressions	1994:2026	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	6	90	theme	chemical	1129:1136	arg1	composition					1138:1148	chemical composition	1129:1148	chemical composition	1129:1148	The efficacies as coating, hydrogel, and scaffold materials were analyzed through cell morphology, proliferation rate, renal-associated gene expressions, chemical composition, and microstructure evaluation.					
35127940	11	91	theme	gene	1657:1660	arg1	expressions					1662:1672	Renal-associated gene expressions	1640:1672	Renal-associated gene expressions	1640:1672	Renal-associated gene expressions were significantly enhanced in the renal ECM than other materials.					
35127940	3	92	theme	kidney-derived	546:559	arg1	matrix					575:580	porcine kidney-derived extracellular matrix	538:580	porcine kidney-derived extracellular matrix (renal ECM)	538:592	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	3	92	theme	kidney-derived	546:559	arg1	ECM					589:591	renal ECM	583:591	renal ECM	583:591	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	16	93	theme	renal	2448:2452	arg1	ECM					2454:2456	renal ECM	2448:2456	renal ECM	2448:2456	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	4	94	theme	acetic	895:900	arg1	acid					902:905	0.5 M acetic acid	889:905	0.5 M acetic acid	889:905	METHODS Porcine renal ECM was prepared by decellularization using 1% Triton X-100, solubilized with 0.5 M acetic acid.					
35127940	1	95	theme	Human	158:162	arg1	tool					227:230	a very useful tool	213:230	a very useful tool for kidney-related experiments in vitro/ex vivo	213:278	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	1	95	theme	Human	158:162	arg1	cell					205:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell	147:208	BACKGROUND Human renal proximal tubular epithelial (RPTE) cell is a very useful tool for kidney-related experiments in vitro/ex vivo.					
35127940	13	96	theme	distinct	1927:1934	arg1	material					1898:1905	the hydrogel material	1885:1905	the hydrogel material	1885:1905	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	13	96	theme	distinct	1927:1934	arg1	morphology					1955:1964	a distinct inner cell network morphology	1925:1964	a distinct inner cell network morphology	1925:1964	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	3	97	theme	cell	766:769	arg1	niche					735:739	three-dimensional (3D) niche	712:739	three-dimensional (3D) niche	712:739	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	3	97	theme	cell	766:769	arg1	vehicle					780:786	a valuable cell delivery vehicle	755:786	a valuable cell delivery vehicle	755:786	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	12	98	theme	effective	1832:1840	arg1	concentration					1842:1854	the effective concentration	1828:1854	the effective concentration	1828:1854	Coating effect on cell proliferation was dependent on the renal ECM concentration, and the effective concentration ranged from 30 to 100 μg.					
35127940	16	99	theme	3D	2473:2474	arg1	niche					2484:2488	useful 3D culture niche and cell delivery vehicles	2466:2515	niche	2484:2488	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	14	100	theme	gene	2193:2196	arg1	expression					2198:2207	renal-associated gene expression	2176:2207	renal-associated gene expression	2176:2207	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	100	theme	gene	2193:2196	arg1	peaks					2112:2116	specific amide peaks	2097:2116	specific amide peaks	2097:2116	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	100	theme	gene	2193:2196	arg1	rate					2166:2169	cell proliferation rate	2147:2169	cell proliferation rate	2147:2169	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	100	theme	gene	2193:2196	arg1	material					2070:2077	the scaffold material	2057:2077	the scaffold material	2057:2077	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	100	theme	gene	2193:2196	arg1	porosity					2137:2144	enhanced internal porosity	2119:2144	enhanced internal porosity	2119:2144	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	0	101	theme	Renal	107:111	arg1	Cell					141:144	Renal Proximal Tubular Epithelial Cell	107:144	Renal Proximal Tubular Epithelial Cell	107:144	Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell.					
35127940	13	102	theme	inner	1936:1940	arg1	material					1898:1905	the hydrogel material	1885:1905	the hydrogel material	1885:1905	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	13	102	theme	inner	1936:1940	arg1	morphology					1955:1964	a distinct inner cell network morphology	1925:1964	a distinct inner cell network morphology	1925:1964	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	12	103	theme	renal	1799:1803	arg1	concentration					1809:1821	the renal ECM concentration	1795:1821	the renal ECM concentration	1795:1821	Coating effect on cell proliferation was dependent on the renal ECM concentration, and the effective concentration ranged from 30 to 100 μg.					
35127940	16	104	theme	renal	2529:2533	arg1	properties					2540:2549	renal cell properties	2529:2549	renal cell properties	2529:2549	More practically, the coated renal ECM stimulates RPTE cell proliferation, and the hydrogel and scaffold of renal ECM provide useful 3D culture niche and cell delivery vehicles maintaining renal cell properties.					
35127940	2	105	theme	artificial	466:475	arg1	conditions					477:486	artificial conditions	466:486	artificial conditions	466:486	However, only a few primary RPTE cells can be obtained through kidney biopsy, the proliferation rate of primary cell is very low, and the cultured cell properties are easily altered in artificial conditions.					
35127940	4	106	dep	METHODS	789:795	arg1	ECM					811:813	Porcine renal ECM	797:813	METHODS Porcine renal ECM	789:813	METHODS Porcine renal ECM was prepared by decellularization using 1% Triton X-100, solubilized with 0.5 M acetic acid.					
35127940	13	107	theme	network	1947:1953	arg1	material					1898:1905	the hydrogel material	1885:1905	the hydrogel material	1885:1905	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	13	107	theme	network	1947:1953	arg1	morphology					1955:1964	a distinct inner cell network morphology	1925:1964	a distinct inner cell network morphology	1925:1964	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	15	108	theme	RPTE	2311:2314	arg1	culture					2321:2327	RPTE cell culture	2311:2327	RPTE cell culture	2311:2327	CONCLUSIONS We concluded that renal ECM can be a suitable material for RPTE cell culture and usage.					
35127940	7	109	theme	collagen	1248:1255	arg1	type					1257:1260	collagen type 1	1248:1262	collagen type 1 (col1)	1248:1269	The efficacies as a coating material were compared with Matrigel, collagen type 1 (col1), gelatin, fibrinogen, and thrombin.					
35127940	7	109	theme	collagen	1248:1255	arg1	col1					1265:1268	col1	1265:1268	col1	1265:1268	The efficacies as a coating material were compared with Matrigel, collagen type 1 (col1), gelatin, fibrinogen, and thrombin.					
35127940	0	110	theme	Kidney-Derived	23:36	arg1	Matrix					52:57	Porcine Kidney-Derived Extracellular Matrix	15:57	Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material	15:101	Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell.					
35127940	14	111	theme	cell	2147:2150	arg1	peaks					2112:2116	specific amide peaks	2097:2116	specific amide peaks	2097:2116	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	111	theme	cell	2147:2150	arg1	expression					2198:2207	renal-associated gene expression	2176:2207	renal-associated gene expression	2176:2207	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	111	theme	cell	2147:2150	arg1	rate					2166:2169	cell proliferation rate	2147:2169	cell proliferation rate	2147:2169	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	111	theme	cell	2147:2150	arg1	material					2070:2077	the scaffold material	2057:2077	the scaffold material	2057:2077	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	111	theme	cell	2147:2150	arg1	porosity					2137:2144	enhanced internal porosity	2119:2144	enhanced internal porosity	2119:2144	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	10	112	theme	cell	1566:1569	arg1	rate					1585:1588	a higher cell proliferation rate	1557:1588	a higher cell proliferation rate	1557:1588	RESULTS As the coating material, renal ECM showed a higher cell proliferation rate compared to other materials, except for Matrigel.					
35127940	0	113	theme	Matrix	52:57	arg1	Application					0:10	Application	0:10	Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell.	0:145	Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell.					
35127940	2	114	theme	cultured	419:426	arg1	properties					433:442	the cultured cell properties	415:442	the cultured cell properties	415:442	However, only a few primary RPTE cells can be obtained through kidney biopsy, the proliferation rate of primary cell is very low, and the cultured cell properties are easily altered in artificial conditions.					
35127940	3	115	theme	renal	583:587	arg1	matrix					575:580	porcine kidney-derived extracellular matrix	538:580	porcine kidney-derived extracellular matrix (renal ECM)	538:592	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	3	115	theme	renal	583:587	arg1	ECM					589:591	renal ECM	583:591	renal ECM	583:591	Thus, RPTE cell usage is very tricky; we applied porcine kidney-derived extracellular matrix (renal ECM) as coating, hydrogel, and scaffold material to increase cell proliferation and maintain cellular properties providing three-dimensional (3D) niche, which can be a valuable cell delivery vehicle.					
35127940	13	116	theme	renal	1908:1912	arg1	ECM					1914:1916	renal ECM	1908:1916	renal ECM	1908:1916	As the hydrogel material, renal ECM showed a distinct inner cell network morphology and significantly increased renal-associated gene expressions, compared to HA hydrogel.					
35127940	15	117	dep	CONCLUSIONS	2240:2250	arg1	concluded					2255:2263	concluded	2255:2263	concluded that renal ECM can be a suitable material for RPTE cell culture and usage	2255:2337	CONCLUSIONS We concluded that renal ECM can be a suitable material for RPTE cell culture and usage.					
35127940	15	118	theme	suitable	2289:2296	arg1	material					2298:2305	a suitable material	2287:2305	a suitable material for RPTE cell culture and usage	2287:2337	CONCLUSIONS We concluded that renal ECM can be a suitable material for RPTE cell culture and usage.					
35127940	15	118	theme	suitable	2289:2296	arg1	ECM					2276:2278	renal ECM	2270:2278	renal ECM	2270:2278	CONCLUSIONS We concluded that renal ECM can be a suitable material for RPTE cell culture and usage.					
35127940	12	119	theme	cell	1759:1762	arg1	proliferation					1764:1776	cell proliferation	1759:1776	cell proliferation	1759:1776	Coating effect on cell proliferation was dependent on the renal ECM concentration, and the effective concentration ranged from 30 to 100 μg.					
35127940	5	120	theme	final	912:916	arg1	concentration					926:938	The final protein concentration	908:938	The final protein concentration	908:938	The final protein concentration was adjusted to 10 μg/μL (pH 7.0).					
35127940	2	121	theme	primary	301:307	arg1	cells					314:318	only a few primary RPTE cells	290:318	only a few primary RPTE cells	290:318	However, only a few primary RPTE cells can be obtained through kidney biopsy, the proliferation rate of primary cell is very low, and the cultured cell properties are easily altered in artificial conditions.					
35127940	6	122	theme	scaffold	1016:1023	arg1	materials					1025:1033	scaffold materials	1016:1033	scaffold materials	1016:1033	The efficacies as coating, hydrogel, and scaffold materials were analyzed through cell morphology, proliferation rate, renal-associated gene expressions, chemical composition, and microstructure evaluation.					
35127940	14	123	theme	internal	2128:2135	arg1	expression					2198:2207	renal-associated gene expression	2176:2207	renal-associated gene expression	2176:2207	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	123	theme	internal	2128:2135	arg1	peaks					2112:2116	specific amide peaks	2097:2116	specific amide peaks	2097:2116	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	123	theme	internal	2128:2135	arg1	rate					2166:2169	cell proliferation rate	2147:2169	cell proliferation rate	2147:2169	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	123	theme	internal	2128:2135	arg1	material					2070:2077	the scaffold material	2057:2077	the scaffold material	2057:2077	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	14	123	theme	internal	2128:2135	arg1	porosity					2137:2144	enhanced internal porosity	2119:2144	enhanced internal porosity	2119:2144	As the scaffold material, renal ECM showed specific amide peaks, enhanced internal porosity, cell proliferation rate, and renal-associated gene expression compared to the col1 scaffold.					
35127940	8	124	theme	effects	1337:1343	arg1	confirmation					1313:1324	confirmation	1313:1324	confirmation of coating effects	1313:1343	After confirmation of coating effects, the effective concentration range was decided.					
37039870	3	0	theme	crops	307:311	arg1	one					268:270	one	268:270	one	268:270	Soybean is one of the most economically important crops in the United States.					
37039870	3	0	theme	crops	307:311	arg1	crops					307:311	the most economically important crops	275:311	the most economically important crops in the United States	275:332	Soybean is one of the most economically important crops in the United States.					
37039870	11	1	theme	Chr	1471:1473	arg1	deletion					1478:1485	this Chr 16 deletion	1466:1485	this Chr 16 deletion	1466:1485	The mapping result using the bi-parental population confirmed that the deletion on Chr 16 conferred elevated sucrose content and a total of 21 genes are located within this Chr 16 deletion.					
37039870	9	2	theme	genome	1019:1024	arg1	WGS					1038:1040	WGS	1038:1040	WGS	1038:1040	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	9	2	theme	genome	1019:1024	arg1	sequencing					1026:1035	whole genome sequencing	1013:1035	whole genome sequencing (WGS)	1013:1041	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	1	3	from	Sucrose	73:79	arg1	seeds					92:96	soybean seeds	84:96	soybean seeds	84:96	Sucrose in soybean seeds is desirable for many end-uses.					
37039870	13	4	theme	genetic	1687:1693	arg1	control					1695:1701	the genetic control	1683:1701	the genetic control of soybean seed sucrose content	1683:1733	The mutation will be useful to understand the genetic control of soybean seed sucrose content.					
37039870	4	5	theme	livestock	371:379	arg1	feed					381:384	livestock feed	371:384	livestock feed	371:384	A primary end-use of soybean is for livestock feed.					
37039870	5	6	theme	genetic	398:404	arg1	improvement					406:416	genetic improvement	398:416	genetic improvement of seed composition	398:436	Therefore, genetic improvement of seed composition is one of the most important goals in soybean breeding programs.					
37039870	10	7	theme	SoySNP50K	1191:1199	arg1	BeadChips					1201:1209	SoySNP50K BeadChips	1191:1209	SoySNP50K BeadChips	1191:1209	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	7	8	theme	elite	595:599	arg1	irradiated					618:627	irradiated	618:627	irradiated	618:627	An elite soybean line was irradiated with fast neutrons and the seed from plants were screened for altered seed composition with near-infrared spectroscopy (NIR).					
37039870	7	8	theme	elite	595:599	arg1	line					609:612	An elite soybean line	592:612	An elite soybean line	592:612	An elite soybean line was irradiated with fast neutrons and the seed from plants were screened for altered seed composition with near-infrared spectroscopy (NIR).					
37039870	6	9	theme	digestible	566:575	arg1	source					584:589	an easily digestible energy source	556:589	an easily digestible energy source	556:589	Sucrose is desired in animal feed due to its role as an easily digestible energy source.					
37039870	11	10	theme	genes	1441:1445	arg1	located					1451:1457	located	1451:1457	located	1451:1457	The mapping result using the bi-parental population confirmed that the deletion on Chr 16 conferred elevated sucrose content and a total of 21 genes are located within this Chr 16 deletion.					
37039870	11	10	theme	genes	1441:1445	arg1	total					1429:1433	a total	1427:1433	a total of 21 genes	1427:1445	The mapping result using the bi-parental population confirmed that the deletion on Chr 16 conferred elevated sucrose content and a total of 21 genes are located within this Chr 16 deletion.					
37039870	2	11	theme	sucrose	140:146	arg1	contents					148:155	Increased sucrose contents	130:155	Increased sucrose contents	130:155	Increased sucrose contents were discovered to associate with a chromosome 16 deletion resulting from fast neutron irradiation.					
37039870	12	12	theme	phenotype	1581:1589	arg1	stability					1564:1572	the stability	1560:1572	the stability of the phenotype across generations in the bi-parental population	1560:1638	NIR and high-pressure liquid chromatography (HPLC) were used to confirm the stability of the phenotype across generations in the bi-parental population.					
37039870	5	13	theme	important	457:465	arg1	goals					467:471	the most important goals	448:471	the most important goals in soybean breeding programs	448:500	Therefore, genetic improvement of seed composition is one of the most important goals in soybean breeding programs.					
37039870	8	14	theme	sucrose	807:813	arg1	content					815:821	higher sucrose content	800:821	higher sucrose content (8-9%)	800:828	One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%).					
37039870	8	14	theme	sucrose	807:813	arg1	%					827:827	8-9%	824:827	8-9%	824:827	One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%).					
37039870	12	15	theme	liquid	1510:1515	arg1	HPLC					1533:1536	HPLC	1533:1536	HPLC	1533:1536	NIR and high-pressure liquid chromatography (HPLC) were used to confirm the stability of the phenotype across generations in the bi-parental population.					
37039870	12	15	theme	liquid	1510:1515	arg1	chromatography					1517:1530	high-pressure liquid chromatography	1496:1530	high-pressure liquid chromatography (HPLC)	1496:1537	NIR and high-pressure liquid chromatography (HPLC) were used to confirm the stability of the phenotype across generations in the bi-parental population.					
37039870	2	16	theme	Increased	130:138	arg1	contents					148:155	Increased sucrose contents	130:155	Increased sucrose contents	130:155	Increased sucrose contents were discovered to associate with a chromosome 16 deletion resulting from fast neutron irradiation.					
37039870	9	17	from	chromosomes	935:945	arg1	mutant					976:981	the mutant	972:981	the mutant	972:981	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	5	18	theme	goals	467:471	arg1	goals					467:471	the most important goals	448:471	the most important goals in soybean breeding programs	448:500	Therefore, genetic improvement of seed composition is one of the most important goals in soybean breeding programs.					
37039870	5	18	theme	goals	467:471	arg1	one					441:443	one	441:443	one	441:443	Therefore, genetic improvement of seed composition is one of the most important goals in soybean breeding programs.					
37039870	8	19	contain	have	795:798	arg1	G15FN-54					772:779	G15FN-54	772:779	G15FN-54	772:779	One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%).					
37039870	8	19	contain	have	795:798	arg2	content					815:821	higher sucrose content	800:821	higher sucrose content (8-9%)	800:828	One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%).					
37039870	8	19	contain	have	795:798	arg1	line					766:769	One mutant line	755:769	One mutant line	755:769	One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%).					
37039870	8	19	contain	have	795:798	arg2	%					827:827	8-9%	824:827	8-9%	824:827	One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%).					
37039870	11	20	theme	elevated	1398:1405	arg1	content					1415:1421	elevated sucrose content	1398:1421	elevated sucrose content	1398:1421	The mapping result using the bi-parental population confirmed that the deletion on Chr 16 conferred elevated sucrose content and a total of 21 genes are located within this Chr 16 deletion.					
37039870	10	21	with	analysis	1171:1178	arg1	BeadChips					1201:1209	SoySNP50K BeadChips	1191:1209	SoySNP50K BeadChips	1191:1209	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	0	22	theme	chromosome	2:11	arg1	deletion					16:23	A chromosome 16 deletion	0:23	A chromosome 16 deletion	0:23	A chromosome 16 deletion conferring a high sucrose phenotype in soybean.					
37039870	11	23	theme	sucrose	1407:1413	arg1	content					1415:1421	elevated sucrose content	1398:1421	elevated sucrose content	1398:1421	The mapping result using the bi-parental population confirmed that the deletion on Chr 16 conferred elevated sucrose content and a total of 21 genes are located within this Chr 16 deletion.					
37039870	9	24	from	deletions	922:930	arg1	13					958:959	13	958:959	13	958:959	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	9	24	from	deletions	922:930	arg1	16					966:967	16	966:967	16	966:967	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	9	24	from	deletions	922:930	arg1	10					954:955	10	954:955	10	954:955	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	9	24	from	deletions	922:930	arg1	Chrs					948:951	Chrs	948:951	Chrs	948:951	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	9	24	from	deletions	922:930	arg1	chromosomes					935:945	chromosomes	935:945	chromosomes	935:945	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	5	25	theme	soybean	476:482	arg1	programs					493:500	soybean breeding programs	476:500	soybean breeding programs	476:500	Therefore, genetic improvement of seed composition is one of the most important goals in soybean breeding programs.					
37039870	9	26	theme	Comparative	861:871	arg1	CGH					896:898	CGH	896:898	CGH	896:898	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	9	26	theme	Comparative	861:871	arg1	hybridization					881:893	Comparative genomic hybridization	861:893	Comparative genomic hybridization (CGH)	861:899	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	10	27	theme	altered	1279:1285	arg1	phenotype					1287:1295	the altered phenotype	1275:1295	the altered phenotype	1275:1295	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	2	28	theme	chromosome	193:202	arg1	deletion					207:214	a chromosome 16 deletion	191:214	a chromosome 16 deletion resulting from fast neutron irradiation	191:254	Increased sucrose contents were discovered to associate with a chromosome 16 deletion resulting from fast neutron irradiation.					
37039870	10	29	theme	segregant	1161:1169	arg1	BSA					1181:1183	BSA	1181:1183	BSA	1181:1183	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	10	29	theme	segregant	1161:1169	arg1	analysis					1171:1178	a bulked segregant analysis	1152:1178	a bulked segregant analysis (BSA) with SoySNP50K BeadChips	1152:1209	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	12	30	from	stability	1564:1572	arg1	population					1629:1638	the bi-parental population	1613:1638	the bi-parental population	1613:1638	NIR and high-pressure liquid chromatography (HPLC) were used to confirm the stability of the phenotype across generations in the bi-parental population.					
37039870	9	31	theme	genomic	873:879	arg1	CGH					896:898	CGH	896:898	CGH	896:898	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	9	31	theme	genomic	873:879	arg1	hybridization					881:893	Comparative genomic hybridization	861:893	Comparative genomic hybridization (CGH)	861:899	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	13	32	theme	soybean	1706:1712	arg1	content					1727:1733	soybean seed sucrose content	1706:1733	soybean seed sucrose content	1706:1733	The mutation will be useful to understand the genetic control of soybean seed sucrose content.					
37039870	4	33	theme	soybean	356:362	arg1	end-use					345:351	A primary end-use	335:351	A primary end-use of soybean	335:362	A primary end-use of soybean is for livestock feed.					
37039870	1	34	theme	soybean	84:90	arg1	seeds					92:96	soybean seeds	84:96	soybean seeds	84:96	Sucrose in soybean seeds is desirable for many end-uses.					
37039870	10	35	theme	bulked	1154:1159	arg1	BSA					1181:1183	BSA	1181:1183	BSA	1181:1183	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	10	35	theme	bulked	1154:1159	arg1	analysis					1171:1178	a bulked segregant analysis	1152:1178	a bulked segregant analysis (BSA) with SoySNP50K BeadChips	1152:1209	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	13	36	theme	seed	1714:1717	arg1	content					1727:1733	soybean seed sucrose content	1706:1733	soybean seed sucrose content	1706:1733	The mutation will be useful to understand the genetic control of soybean seed sucrose content.					
37039870	7	37	from	plants	666:671	arg1	seed					656:659	seed	656:659	seed	656:659	An elite soybean line was irradiated with fast neutrons and the seed from plants were screened for altered seed composition with near-infrared spectroscopy (NIR).					
37039870	12	38	used	used	1544:1547	arg2	NIR					1488:1490	NIR	1488:1490	NIR	1488:1490	NIR and high-pressure liquid chromatography (HPLC) were used to confirm the stability of the phenotype across generations in the bi-parental population.					
37039870	12	38	used	used	1544:1547	arg2	HPLC					1533:1536	HPLC	1533:1536	HPLC	1533:1536	NIR and high-pressure liquid chromatography (HPLC) were used to confirm the stability of the phenotype across generations in the bi-parental population.					
37039870	12	38	used	used	1544:1547	arg2	chromatography					1517:1530	high-pressure liquid chromatography	1496:1530	high-pressure liquid chromatography (HPLC)	1496:1537	NIR and high-pressure liquid chromatography (HPLC) were used to confirm the stability of the phenotype across generations in the bi-parental population.					
37039870	7	39	with	irradiated	618:627	arg1	neutrons					639:646	fast neutrons	634:646	fast neutrons	634:646	An elite soybean line was irradiated with fast neutrons and the seed from plants were screened for altered seed composition with near-infrared spectroscopy (NIR).					
37039870	6	40	theme	energy	577:582	arg1	source					584:589	an easily digestible energy source	556:589	an easily digestible energy source	556:589	Sucrose is desired in animal feed due to its role as an easily digestible energy source.					
37039870	13	41	theme	sucrose	1719:1725	arg1	content					1727:1733	soybean seed sucrose content	1706:1733	soybean seed sucrose content	1706:1733	The mutation will be useful to understand the genetic control of soybean seed sucrose content.					
37039870	10	42	theme	bi-parental	1046:1056	arg1	population					1058:1067	A bi-parental population	1044:1067	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning	1044:1125	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	7	43	theme	near-infrared	721:733	arg1	spectroscopy					735:746	near-infrared spectroscopy	721:746	near-infrared spectroscopy (NIR)	721:752	An elite soybean line was irradiated with fast neutrons and the seed from plants were screened for altered seed composition with near-infrared spectroscopy (NIR).					
37039870	7	43	theme	near-infrared	721:733	arg1	NIR					749:751	NIR	749:751	NIR	749:751	An elite soybean line was irradiated with fast neutrons and the seed from plants were screened for altered seed composition with near-infrared spectroscopy (NIR).					
37039870	0	44	theme	sucrose	43:49	arg1	phenotype					51:59	a high sucrose phenotype	36:59	a high sucrose phenotype in soybean	36:70	A chromosome 16 deletion conferring a high sucrose phenotype in soybean.					
37039870	13	45	theme	content	1727:1733	arg1	control					1695:1701	the genetic control	1683:1701	the genetic control of soybean seed sucrose content	1683:1733	The mutation will be useful to understand the genetic control of soybean seed sucrose content.					
37039870	0	46	from	phenotype	51:59	arg1	soybean					64:70	soybean	64:70	soybean	64:70	A chromosome 16 deletion conferring a high sucrose phenotype in soybean.					
37039870	12	47	theme	bi-parental	1617:1627	arg1	population					1629:1638	the bi-parental population	1613:1638	the bi-parental population	1613:1638	NIR and high-pressure liquid chromatography (HPLC) were used to confirm the stability of the phenotype across generations in the bi-parental population.					
37039870	11	48	theme	bi-parental	1327:1337	arg1	population					1339:1348	the bi-parental population	1323:1348	the bi-parental population	1323:1348	The mapping result using the bi-parental population confirmed that the deletion on Chr 16 conferred elevated sucrose content and a total of 21 genes are located within this Chr 16 deletion.					
37039870	0	49	theme	high	38:41	arg1	phenotype					51:59	a high sucrose phenotype	36:59	a high sucrose phenotype in soybean	36:70	A chromosome 16 deletion conferring a high sucrose phenotype in soybean.					
37039870	3	50	theme	important	297:305	arg1	crops					307:311	the most economically important crops	275:311	the most economically important crops in the United States	275:332	Soybean is one of the most economically important crops in the United States.					
37039870	3	51	from	crops	307:311	arg1	States					327:332	the United States	316:332	the United States	316:332	Soybean is one of the most economically important crops in the United States.					
37039870	4	52	theme	primary	337:343	arg1	end-use					345:351	A primary end-use	335:351	A primary end-use of soybean	335:362	A primary end-use of soybean is for livestock feed.					
37039870	10	53	theme	mutant	1086:1091	arg1	G15FN-54					1093:1100	the mutant G15FN-54	1082:1100	the mutant G15FN-54	1082:1100	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	5	54	from	goals	467:471	arg1	programs					493:500	soybean breeding programs	476:500	soybean breeding programs	476:500	Therefore, genetic improvement of seed composition is one of the most important goals in soybean breeding programs.					
37039870	7	55	theme	fast	634:637	arg1	neutrons					639:646	fast neutrons	634:646	fast neutrons	634:646	An elite soybean line was irradiated with fast neutrons and the seed from plants were screened for altered seed composition with near-infrared spectroscopy (NIR).					
37039870	8	56	theme	parental	839:846	arg1	line					848:851	the parental line	835:851	the parental line (5-6%)	835:858	One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%).					
37039870	8	56	theme	parental	839:846	arg1	%					857:857	5-6%	854:857	5-6%	854:857	One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%).					
37039870	10	57	from	deletion	1231:1238	arg1	Chr					1243:1245	Chr 16	1243:1248	Chr 16	1243:1248	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	5	58	theme	seed	421:424	arg1	composition					426:436	seed composition	421:436	seed composition	421:436	Therefore, genetic improvement of seed composition is one of the most important goals in soybean breeding programs.					
37039870	7	59	theme	seed	699:702	arg1	composition					704:714	altered seed composition	691:714	altered seed composition	691:714	An elite soybean line was irradiated with fast neutrons and the seed from plants were screened for altered seed composition with near-infrared spectroscopy (NIR).					
37039870	10	60	attach	derived	1069:1075	arg2	population					1058:1067	A bi-parental population	1044:1067	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning	1044:1125	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	10	60	attach	derived	1069:1075	arg1	Benning					1119:1125	the cultivar Benning	1106:1125	the cultivar Benning	1106:1125	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	10	60	attach	derived	1069:1075	arg1	G15FN-54					1093:1100	the mutant G15FN-54	1082:1100	the mutant G15FN-54	1082:1100	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	9	61	theme	large	916:920	arg1	deletions					922:930	three large deletions	910:930	three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS)	910:1041	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	5	62	theme	breeding	484:491	arg1	programs					493:500	soybean breeding programs	476:500	soybean breeding programs	476:500	Therefore, genetic improvement of seed composition is one of the most important goals in soybean breeding programs.					
37039870	1	63	theme	many	115:118	arg1	end-uses					120:127	many end-uses	115:127	many end-uses	115:127	Sucrose in soybean seeds is desirable for many end-uses.					
37039870	5	64	theme	composition	426:436	arg1	improvement					406:416	genetic improvement	398:416	genetic improvement of seed composition	398:436	Therefore, genetic improvement of seed composition is one of the most important goals in soybean breeding programs.					
37039870	11	65	theme	mapping	1302:1308	arg1	result					1310:1315	The mapping result	1298:1315	The mapping result using the bi-parental population	1298:1348	The mapping result using the bi-parental population confirmed that the deletion on Chr 16 conferred elevated sucrose content and a total of 21 genes are located within this Chr 16 deletion.					
37039870	6	66	theme	animal	525:530	arg1	feed					532:535	animal feed	525:535	animal feed due to its role as an easily digestible energy source	525:589	Sucrose is desired in animal feed due to its role as an easily digestible energy source.					
37039870	11	67	located	located	1451:1457	arg1	deletion					1478:1485	this Chr 16 deletion	1466:1485	this Chr 16 deletion	1466:1485	The mapping result using the bi-parental population confirmed that the deletion on Chr 16 conferred elevated sucrose content and a total of 21 genes are located within this Chr 16 deletion.					
37039870	11	67	located	located	1451:1457	arg2	total					1429:1433	a total	1427:1433	a total of 21 genes	1427:1445	The mapping result using the bi-parental population confirmed that the deletion on Chr 16 conferred elevated sucrose content and a total of 21 genes are located within this Chr 16 deletion.					
37039870	11	67	located	located	1451:1457	arg2	located					1451:1457	located	1451:1457	located	1451:1457	The mapping result using the bi-parental population confirmed that the deletion on Chr 16 conferred elevated sucrose content and a total of 21 genes are located within this Chr 16 deletion.					
37039870	2	68	theme	neutron	236:242	arg1	irradiation					244:254	fast neutron irradiation	231:254	fast neutron irradiation	231:254	Increased sucrose contents were discovered to associate with a chromosome 16 deletion resulting from fast neutron irradiation.					
37039870	12	69	theme	high-pressure	1496:1508	arg1	HPLC					1533:1536	HPLC	1533:1536	HPLC	1533:1536	NIR and high-pressure liquid chromatography (HPLC) were used to confirm the stability of the phenotype across generations in the bi-parental population.					
37039870	12	69	theme	high-pressure	1496:1508	arg1	chromatography					1517:1530	high-pressure liquid chromatography	1496:1530	high-pressure liquid chromatography (HPLC)	1496:1537	NIR and high-pressure liquid chromatography (HPLC) were used to confirm the stability of the phenotype across generations in the bi-parental population.					
37039870	8	70	theme	higher	800:805	arg1	content					815:821	higher sucrose content	800:821	higher sucrose content (8-9%)	800:828	One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%).					
37039870	8	70	theme	higher	800:805	arg1	%					827:827	8-9%	824:827	8-9%	824:827	One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%).					
37039870	8	71	theme	mutant	759:764	arg1	G15FN-54					772:779	G15FN-54	772:779	G15FN-54	772:779	One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%).					
37039870	8	71	theme	mutant	759:764	arg1	line					766:769	One mutant line	755:769	One mutant line	755:769	One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%).					
37039870	6	72	theme	due	537:539	arg1	feed					532:535	animal feed	525:535	animal feed due to its role as an easily digestible energy source	525:589	Sucrose is desired in animal feed due to its role as an easily digestible energy source.					
37039870	3	73	theme	United	320:325	arg1	States					327:332	the United States	316:332	the United States	316:332	Soybean is one of the most economically important crops in the United States.					
37039870	7	74	theme	soybean	601:607	arg1	irradiated					618:627	irradiated	618:627	irradiated	618:627	An elite soybean line was irradiated with fast neutrons and the seed from plants were screened for altered seed composition with near-infrared spectroscopy (NIR).					
37039870	7	74	theme	soybean	601:607	arg1	line					609:612	An elite soybean line	592:612	An elite soybean line	592:612	An elite soybean line was irradiated with fast neutrons and the seed from plants were screened for altered seed composition with near-infrared spectroscopy (NIR).					
37039870	2	75	theme	fast	231:234	arg1	irradiation					244:254	fast neutron irradiation	231:254	fast neutron irradiation	231:254	Increased sucrose contents were discovered to associate with a chromosome 16 deletion resulting from fast neutron irradiation.					
37039870	9	76	theme	whole	1013:1017	arg1	WGS					1038:1040	WGS	1038:1040	WGS	1038:1040	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	9	76	theme	whole	1013:1017	arg1	sequencing					1026:1035	whole genome sequencing	1013:1035	whole genome sequencing (WGS)	1013:1041	Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS).					
37039870	11	77	from	deletion	1369:1376	arg1	Chr					1381:1383	Chr 16	1381:1386	Chr 16	1381:1386	The mapping result using the bi-parental population confirmed that the deletion on Chr 16 conferred elevated sucrose content and a total of 21 genes are located within this Chr 16 deletion.					
37039870	10	78	theme	cultivar	1110:1117	arg1	Benning					1119:1125	the cultivar Benning	1106:1125	the cultivar Benning	1106:1125	A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype.					
37039870	7	79	theme	altered	691:697	arg1	composition					704:714	altered seed composition	691:714	altered seed composition	691:714	An elite soybean line was irradiated with fast neutrons and the seed from plants were screened for altered seed composition with near-infrared spectroscopy (NIR).					
36001029	6	0	link	derived	841:847	arg1	composition					849:859	nature derived composition	834:859	nature derived composition	834:859	The biomimetic structure and nature derived composition endow the microspheres with excellent biocompatibility and enhanced osteogenic differentiation-inducing ability to mesenchymal stem cells.					
36001029	6	1	theme	stem	988:991	arg1	cells					993:997	mesenchymal stem cells	976:997	mesenchymal stem cells	976:997	The biomimetic structure and nature derived composition endow the microspheres with excellent biocompatibility and enhanced osteogenic differentiation-inducing ability to mesenchymal stem cells.					
36001029	1	2	with	class	167:171	arg1	applications					245:256	unexplored biomedical applications	223:256	unexplored biomedical applications	223:256	Silk nanofibrils (SNFs) extracted from natural silkworm silk represent a class of high-potential protein nanofiber material with unexplored biomedical applications.					
36001029	6	3	theme	enhanced	920:927	arg1	ability					965:971	enhanced osteogenic differentiation-inducing ability	920:971	enhanced osteogenic differentiation-inducing ability	920:971	The biomimetic structure and nature derived composition endow the microspheres with excellent biocompatibility and enhanced osteogenic differentiation-inducing ability to mesenchymal stem cells.					
36001029	8	4	from	level	1210:1214	arg1	modifiability					1177:1189	modifiability	1177:1189	modifiability	1177:1189	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	8	4	from	level	1210:1214	arg1	compatibility					1159:1171	the excellent blood compatibility	1139:1171	the excellent blood compatibility	1139:1171	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	8	4	from	level	1210:1214	arg1	advantages					1125:1134	the advantages	1121:1134	the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres	1121:1251	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	8	5	theme	blood	1153:1157	arg1	compatibility					1159:1171	the excellent blood compatibility	1139:1171	the excellent blood compatibility	1139:1171	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	3	6	from	silks	442:446	arg1	exfoliated					417:426	exfoliated	417:426	exfoliated	417:426	The SNFs were exfoliated from silkworm silks through an all-aqueous process and used as the building blocks for constructing the microspheres.					
36001029	6	7	theme	derived	841:847	arg1	composition					849:859	nature derived composition	834:859	nature derived composition	834:859	The biomimetic structure and nature derived composition endow the microspheres with excellent biocompatibility and enhanced osteogenic differentiation-inducing ability to mesenchymal stem cells.					
36001029	4	8	theme	bioactive	576:584	arg1	composition					586:596	bioactive composition	576:596	bioactive composition	576:596	Inspired by the structure and bioactive composition of ECM, hyaluronic acid (HA) was used as a bio-glue to regulate SNF assembly.					
36001029	4	9	theme	hyaluronic	606:615	arg1	HA					623:624	HA	623:624	HA	623:624	Inspired by the structure and bioactive composition of ECM, hyaluronic acid (HA) was used as a bio-glue to regulate SNF assembly.					
36001029	4	9	theme	hyaluronic	606:615	arg1	acid					617:620	hyaluronic acid	606:620	hyaluronic acid (HA)	606:625	Inspired by the structure and bioactive composition of ECM, hyaluronic acid (HA) was used as a bio-glue to regulate SNF assembly.					
36001029	4	9	theme	hyaluronic	606:615	arg1	bio-glue					641:648	a bio-glue	639:648	a bio-glue	639:648	Inspired by the structure and bioactive composition of ECM, hyaluronic acid (HA) was used as a bio-glue to regulate SNF assembly.					
36001029	8	10	theme	microspheres	1240:1251	arg1	nanoscale					1223:1231	nanoscale	1223:1231	nanoscale	1223:1231	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	7	11	theme	SNF	1029:1031	arg1	proof					1003:1007	proof	1003:1007	proof of versatility	1003:1022	As proof of versatility, the SNF microspheres were further functionalized with other molecules and nanomaterials.					
36001029	7	11	theme	SNF	1029:1031	arg1	microspheres					1033:1044	the SNF microspheres	1025:1044	the SNF microspheres	1025:1044	As proof of versatility, the SNF microspheres were further functionalized with other molecules and nanomaterials.					
36001029	9	12	theme	disease	1514:1520	arg1	diagnosis					1522:1530	early disease diagnosis	1508:1530	early disease diagnosis	1508:1530	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	5	13	theme	stable	729:734	arg1	structures					755:764	stable fluffy nanofibrous structures	729:764	stable fluffy nanofibrous structures	729:764	With the assistance of HA, the SNF microspheres with stable fluffy nanofibrous structures were synthesized through electrospray.					
36001029	7	14	theme	versatility	1012:1022	arg1	proof					1003:1007	proof	1003:1007	proof of versatility	1003:1022	As proof of versatility, the SNF microspheres were further functionalized with other molecules and nanomaterials.					
36001029	7	14	theme	versatility	1012:1022	arg1	microspheres					1033:1044	the SNF microspheres	1025:1044	the SNF microspheres	1025:1044	As proof of versatility, the SNF microspheres were further functionalized with other molecules and nanomaterials.					
36001029	2	15	theme	matrix	321:326	arg1	architecture					344:355	extracellular matrix (ECM)-mimicking architecture	307:355	extracellular matrix (ECM)-mimicking architecture	307:355	In this study, a SNF-assembled microsphere with extracellular matrix (ECM)-mimicking architecture and high specific surface area was developed.					
36001029	4	16	dep	structure	562:570	arg1	the					558:560	the	558:560	the	558:560	Inspired by the structure and bioactive composition of ECM, hyaluronic acid (HA) was used as a bio-glue to regulate SNF assembly.					
36001029	5	17	theme	fluffy	736:741	arg1	structures					755:764	stable fluffy nanofibrous structures	729:764	stable fluffy nanofibrous structures	729:764	With the assistance of HA, the SNF microspheres with stable fluffy nanofibrous structures were synthesized through electrospray.					
36001029	6	18	theme	differentiation-inducing	940:963	arg1	ability					965:971	enhanced osteogenic differentiation-inducing ability	920:971	enhanced osteogenic differentiation-inducing ability	920:971	The biomimetic structure and nature derived composition endow the microspheres with excellent biocompatibility and enhanced osteogenic differentiation-inducing ability to mesenchymal stem cells.					
36001029	3	19	theme	building	495:502	arg1	blocks					504:509	the building blocks	491:509	the building blocks	491:509	The SNFs were exfoliated from silkworm silks through an all-aqueous process and used as the building blocks for constructing the microspheres.					
36001029	3	19	theme	building	495:502	arg1	SNFs					407:410	The SNFs	403:410	The SNFs	403:410	The SNFs were exfoliated from silkworm silks through an all-aqueous process and used as the building blocks for constructing the microspheres.					
36001029	1	20	theme	Silk	94:97	arg1	SNFs					112:115	SNFs	112:115	SNFs	112:115	Silk nanofibrils (SNFs) extracted from natural silkworm silk represent a class of high-potential protein nanofiber material with unexplored biomedical applications.					
36001029	1	20	theme	Silk	94:97	arg1	nanofibrils					99:109	Silk nanofibrils	94:109	Silk nanofibrils (SNFs) extracted from natural silkworm silk	94:153	Silk nanofibrils (SNFs) extracted from natural silkworm silk represent a class of high-potential protein nanofiber material with unexplored biomedical applications.					
36001029	9	21	theme	therapeutic	1537:1547	arg1	devices					1549:1555	therapeutic devices	1537:1555	therapeutic devices	1537:1555	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	1	22	theme	high-potential	176:189	arg1	material					209:216	high-potential protein nanofiber material	176:216	high-potential protein nanofiber material	176:216	Silk nanofibrils (SNFs) extracted from natural silkworm silk represent a class of high-potential protein nanofiber material with unexplored biomedical applications.					
36001029	3	23	theme	silkworm	433:440	arg1	silks					442:446	silkworm silks	433:446	silkworm silks	433:446	The SNFs were exfoliated from silkworm silks through an all-aqueous process and used as the building blocks for constructing the microspheres.					
36001029	6	24	theme	osteogenic	929:938	arg1	ability					965:971	enhanced osteogenic differentiation-inducing ability	920:971	enhanced osteogenic differentiation-inducing ability	920:971	The biomimetic structure and nature derived composition endow the microspheres with excellent biocompatibility and enhanced osteogenic differentiation-inducing ability to mesenchymal stem cells.					
36001029	6	25	theme	biomimetic	809:818	arg1	structure					820:828	The biomimetic structure	805:828	The biomimetic structure	805:828	The biomimetic structure and nature derived composition endow the microspheres with excellent biocompatibility and enhanced osteogenic differentiation-inducing ability to mesenchymal stem cells.					
36001029	1	26	theme	protein	191:197	arg1	material					209:216	high-potential protein nanofiber material	176:216	high-potential protein nanofiber material	176:216	Silk nanofibrils (SNFs) extracted from natural silkworm silk represent a class of high-potential protein nanofiber material with unexplored biomedical applications.					
36001029	0	27	theme	Natural	11:17	arg1	Microspheres					36:47	Biomimetic Natural Silk Nanofibrous Microspheres	0:47	Biomimetic Natural Silk Nanofibrous Microspheres	0:47	Biomimetic Natural Silk Nanofibrous Microspheres for Multifunctional Biomedical Applications.					
36001029	4	28	theme	ECM	601:603	arg1	structure					562:570	structure	562:570	structure	562:570	Inspired by the structure and bioactive composition of ECM, hyaluronic acid (HA) was used as a bio-glue to regulate SNF assembly.					
36001029	4	28	theme	ECM	601:603	arg1	composition					586:596	bioactive composition	576:596	bioactive composition	576:596	Inspired by the structure and bioactive composition of ECM, hyaluronic acid (HA) was used as a bio-glue to regulate SNF assembly.					
36001029	8	29	theme	excellent	1143:1151	arg1	compatibility					1159:1171	the excellent blood compatibility	1139:1171	the excellent blood compatibility	1139:1171	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	1	30	theme	nanofiber	199:207	arg1	material					209:216	high-potential protein nanofiber material	176:216	high-potential protein nanofiber material	176:216	Silk nanofibrils (SNFs) extracted from natural silkworm silk represent a class of high-potential protein nanofiber material with unexplored biomedical applications.					
36001029	0	31	theme	Biomimetic	0:9	arg1	Microspheres					36:47	Biomimetic Natural Silk Nanofibrous Microspheres	0:47	Biomimetic Natural Silk Nanofibrous Microspheres	0:47	Biomimetic Natural Silk Nanofibrous Microspheres for Multifunctional Biomedical Applications.					
36001029	9	32	theme	nanofibrous	1416:1426	arg1	material					1475:1482	a superior biomedical material	1453:1482	a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices	1453:1555	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	9	32	theme	nanofibrous	1416:1426	arg1	microsphere					1428:1438	this natural silk-based nanofibrous microsphere	1392:1438	this natural silk-based nanofibrous microsphere	1392:1438	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	9	33	theme	superior	1455:1462	arg1	material					1475:1482	a superior biomedical material	1453:1482	a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices	1453:1555	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	9	33	theme	superior	1455:1462	arg1	microsphere					1428:1438	this natural silk-based nanofibrous microsphere	1392:1438	this natural silk-based nanofibrous microsphere	1392:1438	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	2	34	theme	-mimicking	333:342	arg1	architecture					344:355	extracellular matrix (ECM)-mimicking architecture	307:355	extracellular matrix (ECM)-mimicking architecture	307:355	In this study, a SNF-assembled microsphere with extracellular matrix (ECM)-mimicking architecture and high specific surface area was developed.					
36001029	1	35	theme	material	209:216	arg1	class					167:171	a class	165:171	a class of high-potential protein nanofiber material with unexplored biomedical applications	165:256	Silk nanofibrils (SNFs) extracted from natural silkworm silk represent a class of high-potential protein nanofiber material with unexplored biomedical applications.					
36001029	0	36	theme	Nanofibrous	24:34	arg1	Microspheres					36:47	Biomimetic Natural Silk Nanofibrous Microspheres	0:47	Biomimetic Natural Silk Nanofibrous Microspheres	0:47	Biomimetic Natural Silk Nanofibrous Microspheres for Multifunctional Biomedical Applications.					
36001029	4	37	used	used	631:634	arg2	HA					623:624	HA	623:624	HA	623:624	Inspired by the structure and bioactive composition of ECM, hyaluronic acid (HA) was used as a bio-glue to regulate SNF assembly.					
36001029	4	37	used	used	631:634	arg2	bio-glue					641:648	a bio-glue	639:648	a bio-glue	639:648	Inspired by the structure and bioactive composition of ECM, hyaluronic acid (HA) was used as a bio-glue to regulate SNF assembly.					
36001029	4	37	used	used	631:634	arg2	acid					617:620	hyaluronic acid	606:620	hyaluronic acid (HA)	606:625	Inspired by the structure and bioactive composition of ECM, hyaluronic acid (HA) was used as a bio-glue to regulate SNF assembly.					
36001029	0	38	theme	Silk	19:22	arg1	Microspheres					36:47	Biomimetic Natural Silk Nanofibrous Microspheres	0:47	Biomimetic Natural Silk Nanofibrous Microspheres	0:47	Biomimetic Natural Silk Nanofibrous Microspheres for Multifunctional Biomedical Applications.					
36001029	9	39	dep	results	1367:1373	arg1	basis					1352:1356	basis	1352:1356	basis	1352:1356	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	9	39	dep	results	1367:1373	arg1	the					1348:1350	the	1348:1350	the	1348:1350	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	2	40	theme	extracellular	307:319	arg1	matrix					321:326	extracellular matrix	307:326	extracellular matrix (ECM)-mimicking architecture	307:355	In this study, a SNF-assembled microsphere with extracellular matrix (ECM)-mimicking architecture and high specific surface area was developed.					
36001029	2	40	theme	extracellular	307:319	arg1	ECM					329:331	ECM	329:331	ECM	329:331	In this study, a SNF-assembled microsphere with extracellular matrix (ECM)-mimicking architecture and high specific surface area was developed.					
36001029	6	41	dep	derived	841:847	arg1	nature					834:839	nature	834:839	nature	834:839	The biomimetic structure and nature derived composition endow the microspheres with excellent biocompatibility and enhanced osteogenic differentiation-inducing ability to mesenchymal stem cells.					
36001029	1	42	theme	unexplored	223:232	arg1	applications					245:256	unexplored biomedical applications	223:256	unexplored biomedical applications	223:256	Silk nanofibrils (SNFs) extracted from natural silkworm silk represent a class of high-potential protein nanofiber material with unexplored biomedical applications.					
36001029	8	43	theme	protease	1302:1309	arg1	detection					1311:1319	protease detection	1302:1319	protease detection	1302:1319	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	2	44	theme	specific	366:373	arg1	area					383:386	high specific surface area	361:386	high specific surface area	361:386	In this study, a SNF-assembled microsphere with extracellular matrix (ECM)-mimicking architecture and high specific surface area was developed.					
36001029	5	45	theme	SNF	707:709	arg1	microspheres					711:722	the SNF microspheres	703:722	the SNF microspheres with stable fluffy nanofibrous structures	703:764	With the assistance of HA, the SNF microspheres with stable fluffy nanofibrous structures were synthesized through electrospray.					
36001029	6	46	with	microspheres	871:882	arg1	ability					965:971	enhanced osteogenic differentiation-inducing ability	920:971	enhanced osteogenic differentiation-inducing ability	920:971	The biomimetic structure and nature derived composition endow the microspheres with excellent biocompatibility and enhanced osteogenic differentiation-inducing ability to mesenchymal stem cells.					
36001029	6	46	with	microspheres	871:882	arg1	biocompatibility					899:914	excellent biocompatibility	889:914	excellent biocompatibility	889:914	The biomimetic structure and nature derived composition endow the microspheres with excellent biocompatibility and enhanced osteogenic differentiation-inducing ability to mesenchymal stem cells.					
36001029	6	47	theme	excellent	889:897	arg1	biocompatibility					899:914	excellent biocompatibility	889:914	excellent biocompatibility	889:914	The biomimetic structure and nature derived composition endow the microspheres with excellent biocompatibility and enhanced osteogenic differentiation-inducing ability to mesenchymal stem cells.					
36001029	3	48	theme	all-aqueous	459:469	arg1	process					471:477	an all-aqueous process	456:477	an all-aqueous process	456:477	The SNFs were exfoliated from silkworm silks through an all-aqueous process and used as the building blocks for constructing the microspheres.					
36001029	1	49	theme	biomedical	234:243	arg1	applications					245:256	unexplored biomedical applications	223:256	unexplored biomedical applications	223:256	Silk nanofibrils (SNFs) extracted from natural silkworm silk represent a class of high-potential protein nanofiber material with unexplored biomedical applications.					
36001029	8	50	theme	blood	1325:1329	arg1	purification					1331:1342	blood purification	1325:1342	blood purification	1325:1342	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	9	51	theme	biomedical	1464:1473	arg1	material					1475:1482	a superior biomedical material	1453:1482	a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices	1453:1555	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	9	51	theme	biomedical	1464:1473	arg1	microsphere					1428:1438	this natural silk-based nanofibrous microsphere	1392:1438	this natural silk-based nanofibrous microsphere	1392:1438	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	3	52	used	used	483:486	arg2	blocks					504:509	the building blocks	491:509	the building blocks	491:509	The SNFs were exfoliated from silkworm silks through an all-aqueous process and used as the building blocks for constructing the microspheres.					
36001029	3	52	used	used	483:486	arg2	SNFs					407:410	The SNFs	403:410	The SNFs	403:410	The SNFs were exfoliated from silkworm silks through an all-aqueous process and used as the building blocks for constructing the microspheres.					
36001029	5	53	theme	nanofibrous	743:753	arg1	structures					755:764	stable fluffy nanofibrous structures	729:764	stable fluffy nanofibrous structures	729:764	With the assistance of HA, the SNF microspheres with stable fluffy nanofibrous structures were synthesized through electrospray.					
36001029	0	54	theme	Biomedical	69:78	arg1	Applications					80:91	Multifunctional Biomedical Applications	53:91	Multifunctional Biomedical Applications	53:91	Biomimetic Natural Silk Nanofibrous Microspheres for Multifunctional Biomedical Applications.					
36001029	9	55	theme	natural	1397:1403	arg1	material					1475:1482	a superior biomedical material	1453:1482	a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices	1453:1555	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	9	55	theme	natural	1397:1403	arg1	microsphere					1428:1438	this natural silk-based nanofibrous microsphere	1392:1438	this natural silk-based nanofibrous microsphere	1392:1438	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	2	56	theme	surface	375:381	arg1	area					383:386	high specific surface area	361:386	high specific surface area	361:386	In this study, a SNF-assembled microsphere with extracellular matrix (ECM)-mimicking architecture and high specific surface area was developed.					
36001029	0	57	theme	Multifunctional	53:67	arg1	Applications					80:91	Multifunctional Biomedical Applications	53:91	Multifunctional Biomedical Applications	53:91	Biomimetic Natural Silk Nanofibrous Microspheres for Multifunctional Biomedical Applications.					
36001029	2	58	with	microsphere	290:300	arg1	area					383:386	high specific surface area	361:386	high specific surface area	361:386	In this study, a SNF-assembled microsphere with extracellular matrix (ECM)-mimicking architecture and high specific surface area was developed.					
36001029	2	58	with	microsphere	290:300	arg1	architecture					344:355	extracellular matrix (ECM)-mimicking architecture	307:355	extracellular matrix (ECM)-mimicking architecture	307:355	In this study, a SNF-assembled microsphere with extracellular matrix (ECM)-mimicking architecture and high specific surface area was developed.					
36001029	8	59	theme	compatibility	1159:1171	arg1	advantages					1125:1134	the advantages	1121:1134	the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres	1121:1251	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	6	60	theme	mesenchymal	976:986	arg1	cells					993:997	mesenchymal stem cells	976:997	mesenchymal stem cells	976:997	The biomimetic structure and nature derived composition endow the microspheres with excellent biocompatibility and enhanced osteogenic differentiation-inducing ability to mesenchymal stem cells.					
36001029	0	61	dep	Microspheres	36:47	arg1	Applications					80:91	Multifunctional Biomedical Applications	53:91	Multifunctional Biomedical Applications	53:91	Biomimetic Natural Silk Nanofibrous Microspheres for Multifunctional Biomedical Applications.					
36001029	1	62	theme	natural	133:139	arg1	silk					150:153	natural silkworm silk	133:153	natural silkworm silk	133:153	Silk nanofibrils (SNFs) extracted from natural silkworm silk represent a class of high-potential protein nanofiber material with unexplored biomedical applications.					
36001029	5	63	with	microspheres	711:722	arg1	structures					755:764	stable fluffy nanofibrous structures	729:764	stable fluffy nanofibrous structures	729:764	With the assistance of HA, the SNF microspheres with stable fluffy nanofibrous structures were synthesized through electrospray.					
36001029	8	64	from	advantages	1125:1134	arg1	level					1210:1214	the molecular level	1196:1214	the molecular level	1196:1214	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	8	65	theme	modifiability	1177:1189	arg1	advantages					1125:1134	the advantages	1121:1134	the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres	1121:1251	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	7	66	theme	other	1079:1083	arg1	molecules					1085:1093	other molecules	1079:1093	other molecules	1079:1093	As proof of versatility, the SNF microspheres were further functionalized with other molecules and nanomaterials.					
36001029	1	67	theme	silkworm	141:148	arg1	silk					150:153	natural silkworm silk	133:153	natural silkworm silk	133:153	Silk nanofibrils (SNFs) extracted from natural silkworm silk represent a class of high-potential protein nanofiber material with unexplored biomedical applications.					
36001029	5	68	theme	HA	699:700	arg1	assistance					685:694	the assistance	681:694	the assistance of HA	681:700	With the assistance of HA, the SNF microspheres with stable fluffy nanofibrous structures were synthesized through electrospray.					
36001029	9	69	theme	early	1508:1512	arg1	diagnosis					1522:1530	early disease diagnosis	1508:1530	early disease diagnosis	1508:1530	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	2	70	theme	SNF-assembled	276:288	arg1	microsphere					290:300	a SNF-assembled microsphere	274:300	a SNF-assembled microsphere with extracellular matrix (ECM)-mimicking architecture and high specific surface area	274:386	In this study, a SNF-assembled microsphere with extracellular matrix (ECM)-mimicking architecture and high specific surface area was developed.					
36001029	2	71	theme	high	361:364	arg1	area					383:386	high specific surface area	361:386	high specific surface area	361:386	In this study, a SNF-assembled microsphere with extracellular matrix (ECM)-mimicking architecture and high specific surface area was developed.					
36001029	4	72	theme	SNF	662:664	arg1	assembly					666:673	SNF assembly	662:673	SNF assembly	662:673	Inspired by the structure and bioactive composition of ECM, hyaluronic acid (HA) was used as a bio-glue to regulate SNF assembly.					
36001029	9	73	theme	tissue	1488:1493	arg1	engineering					1495:1505	tissue engineering	1488:1505	tissue engineering	1488:1505	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	8	74	from	applications	1286:1297	arg1	detection					1311:1319	protease detection	1302:1319	protease detection	1302:1319	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	8	74	from	applications	1286:1297	arg1	purification					1331:1342	blood purification	1325:1342	blood purification	1325:1342	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	8	75	theme	versatile	1276:1284	arg1	applications					1286:1297	their versatile applications	1270:1297	their versatile applications in protease detection and blood purification	1270:1342	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	9	76	theme	silk-based	1405:1414	arg1	material					1475:1482	a superior biomedical material	1453:1482	a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices	1453:1555	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	9	76	theme	silk-based	1405:1414	arg1	microsphere					1428:1438	this natural silk-based nanofibrous microsphere	1392:1438	this natural silk-based nanofibrous microsphere	1392:1438	On the basis of these results, we foresee that this natural silk-based nanofibrous microsphere may serve as a superior biomedical material for tissue engineering, early disease diagnosis, and therapeutic devices.					
36001029	8	77	theme	molecular	1200:1208	arg1	level					1210:1214	the molecular level	1196:1214	the molecular level	1196:1214	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
36001029	8	78	theme	SNF	1236:1238	arg1	microspheres					1240:1251	SNF microspheres	1236:1251	SNF microspheres	1236:1251	Taking the advantages of the excellent blood compatibility and modifiability from the molecular level to the nanoscale of SNF microspheres, we demonstrated their versatile applications in protease detection and blood purification.					
35124796	2	0	theme	randomized	699:708	arg1	design					710:715	a completely randomized design	686:715	a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements)	686:793	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	8	1	theme	additives	1589:1597	arg1	use					1577:1579	The use	1573:1579	The use of feed additives	1573:1597	The use of feed additives had significant effects on the nutrient content of feces (P < 0.05).					
35124796	5	2	theme	insignificant	1222:1234	arg1	effects					1236:1242	insignificant effects	1222:1242	insignificant effects	1222:1242	Decreased dietary crude protein levels in growing and finishing period had insignificant effects on chicken's performance (P > 0.05).					
35124796	1	3	theme	feed	236:239	arg1	additives					241:249	selected natural feed additives	219:249	selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic)	219:305	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	3	theme	feed	236:239	arg1	extract					258:264	thyme extract	252:264	thyme extract	252:264	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	3	theme	feed	236:239	arg1	acid					275:278	organic acid	267:278	organic acid	267:278	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	3	theme	feed	236:239	arg1	prebiotic					296:304	prebiotic	296:304	prebiotic	296:304	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	9	4	theme	adverse	1861:1867	arg1	effects					1869:1875	adverse effects	1861:1875	adverse effects on the performance of chickens	1861:1906	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	2	5	theme	strain	522:527	arg1	broilers					529:536	388 Ross-308 strain broilers	509:536	388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days)	509:671	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	8	6	theme	significant	1603:1613	arg1	effects					1615:1621	significant effects	1603:1621	significant effects	1603:1621	The use of feed additives had significant effects on the nutrient content of feces (P < 0.05).					
35124796	1	7	theme	levels	404:409	arg1	diet					411:414	two crude protein levels diet	386:414	two crude protein levels diet	386:414	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	6	8	theme	additives	1327:1335	arg1	use					1315:1317	the use	1311:1317	the use of feed additives on diets with lower than normal crude protein levels	1311:1388	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	3	9	contain	had	884:886	arg2	morphology					935:944	intestinal morphology	924:944	intestinal morphology	924:944	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	3	9	contain	had	884:886	arg1	use					822:824	use	822:824	use of feed additives along with both levels of crude protein	822:882	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	3	9	contain	had	884:886	arg2	P < 0.05					974:981	P < 0.05	974:981	P < 0.05	974:981	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	3	9	contain	had	884:886	arg2	levels					966:971	fecal nutrient levels	951:971	fecal nutrient levels (P < 0.05)	951:982	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	3	9	contain	had	884:886	arg2	effects					900:906	significant effects	888:906	significant effects on performance	888:921	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	4	10	theme	starting	1119:1126	arg1	P < 0.05					1136:1143	P < 0.05	1136:1143	P < 0.05	1136:1143	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	4	10	theme	starting	1119:1126	arg1	period					1128:1133	the starting period	1115:1133	the starting period (P < 0.05)	1115:1144	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	9	11	from	effects	1869:1875	arg1	performance					1884:1894	the performance	1880:1894	the performance of chickens	1880:1906	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	3	12	theme	protein	876:882	arg1	levels					860:865	both levels	855:865	both levels of crude protein	855:882	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	8	13	theme	nutrient	1630:1637	arg1	content					1639:1645	the nutrient content	1626:1645	the nutrient content of feces (P < 0.05)	1626:1665	The use of feed additives had significant effects on the nutrient content of feces (P < 0.05).					
35124796	1	14	dep	additives	241:249	arg1	additives					241:249	selected natural feed additives	219:249	selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic)	219:305	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	14	dep	additives	241:249	arg1	probiotic					281:289	probiotic	281:289	probiotic	281:289	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	14	dep	additives	241:249	arg1	extract					258:264	thyme extract	252:264	thyme extract	252:264	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	14	dep	additives	241:249	arg1	acid					275:278	organic acid	267:278	organic acid	267:278	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	14	dep	additives	241:249	arg1	prebiotic					296:304	prebiotic	296:304	prebiotic	296:304	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	7	15	theme	crude	1507:1511	arg1	level					1521:1525	Lower crude protein level	1501:1525	Lower crude protein level	1501:1525	Lower crude protein level changed the intestinal morphology (P < 0.05).					
35124796	1	16	theme	crude	390:394	arg1	levels					404:409	two crude protein levels	386:409	two crude protein levels diet	386:414	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	3	17	from	morphology	935:944	arg1	performance					911:921	performance	911:921	performance	911:921	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	9	18	theme	chickens	1899:1906	arg1	performance					1884:1894	the performance	1880:1894	the performance of chickens	1880:1906	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	0	19	theme	Intestinal	107:116	arg1	Morphology					118:127	Intestinal Morphology	107:127	Intestinal Morphology	107:127	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	6	20	theme	protein	1375:1381	arg1	levels					1383:1388	lower than normal crude protein levels	1351:1388	lower than normal crude protein levels	1351:1388	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	8	21	contain	had	1599:1601	arg1	use					1577:1579	The use	1573:1579	The use of feed additives	1573:1597	The use of feed additives had significant effects on the nutrient content of feces (P < 0.05).					
35124796	8	21	contain	had	1599:1601	arg2	effects					1615:1621	significant effects	1603:1621	significant effects	1603:1621	The use of feed additives had significant effects on the nutrient content of feces (P < 0.05).					
35124796	1	22	theme	fecal	365:369	arg1	nutrients					371:379	fecal nutrients	365:379	fecal nutrients	365:379	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	6	23	theme	normal	1362:1367	arg1	levels					1383:1388	lower than normal crude protein levels	1351:1388	lower than normal crude protein levels	1351:1388	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	9	24	theme	normal	1756:1761	arg1	levels					1777:1782	normal crude protein levels	1756:1782	normal crude protein levels	1756:1782	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	6	25	theme	daily	1432:1436	arg1	gain					1445:1448	daily weight gain	1432:1448	daily weight gain	1432:1448	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	4	26	theme	crude	1003:1007	arg1	level					1017:1021	crude protein level	1003:1021	crude protein level of diet	1003:1029	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	1	27	from	effects	208:214	arg1	composition					350:360	composition	350:360	composition	350:360	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	27	from	effects	208:214	arg1	performance					310:320	performance	310:320	performance	310:320	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	27	from	effects	208:214	arg1	morphology					334:343	intestinal morphology	323:343	intestinal morphology	323:343	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	9	28	theme	%	1701:1701	arg1	reduction					1703:1711	a 10% reduction	1697:1711	a 10% reduction in crude protein level of diet compared to normal crude protein levels	1697:1782	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	0	29	theme	Thymus	49:54	arg1	vulgaris					56:63	Thymus vulgaris	49:63	Thymus vulgaris	49:63	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	0	29	theme	Thymus	49:54	arg1	Prebiotics					14:23	Prebiotics	14:23	Prebiotics	14:23	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	6	30	dep	normal	1362:1367	arg1	lower					1351:1355	lower	1351:1355	lower	1351:1355	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	6	30	dep	normal	1362:1367	arg1	than					1357:1360	than	1357:1360	than	1357:1360	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	4	31	theme	10	987:988	arg1	%					989:989	%	989:989	%	989:989	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	6	32	theme	feed	1419:1422	arg1	intake					1424:1429	the average feed intake	1407:1429	the average feed intake	1407:1429	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	9	33	theme	crude	1763:1767	arg1	levels					1777:1782	normal crude protein levels	1756:1782	normal crude protein levels	1756:1782	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	2	34	dep	design	710:715	arg1	replicates					734:743	replicates	734:743	replicates, and 12 chickens in 5 × 2 factorial arrangements	734:792	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	3	35	theme	significant	888:898	arg1	effects					900:906	significant effects	888:906	significant effects on performance	888:921	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	6	36	from	use	1315:1317	arg1	diets					1340:1344	diets	1340:1344	diets with lower than normal crude protein levels	1340:1388	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	1	37	theme	intestinal	323:332	arg1	morphology					334:343	intestinal morphology	323:343	intestinal morphology	323:343	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	0	38	dep	Oil	76:78	arg1	Performance					94:104	Performance	94:104	Performance	94:104	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	0	38	dep	Oil	76:78	arg1	Morphology					118:127	Intestinal Morphology	107:127	Intestinal Morphology	107:127	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	0	38	dep	Oil	76:78	arg1	Composition					149:159	Fecal Nutrient Composition	134:159	Fecal Nutrient Composition	134:159	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	2	39	dep	24 days	634:640	arg1	to					631:632	to	631:632	to	631:632	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	6	40	theme	conversion	1460:1469	arg1	ratio					1471:1475	feed conversion ratio	1455:1475	feed conversion ratio	1455:1475	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	0	41	theme	Supplementary	0:12	arg1	Oil					76:78	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil	0:78	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.	0:160	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	1	42	theme	thyme	252:256	arg1	additives					241:249	selected natural feed additives	219:249	selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic)	219:305	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	42	theme	thyme	252:256	arg1	extract					258:264	thyme extract	252:264	thyme extract	252:264	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	8	43	theme	feed	1584:1587	arg1	additives					1589:1597	feed additives	1584:1597	feed additives	1584:1597	The use of feed additives had significant effects on the nutrient content of feces (P < 0.05).					
35124796	4	44	theme	diet	1026:1029	arg1	level					1017:1021	crude protein level	1003:1021	crude protein level of diet	1003:1029	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	5	45	contain	had	1218:1220	arg1	levels					1179:1184	Decreased dietary crude protein levels	1147:1184	Decreased dietary crude protein levels in growing and finishing period	1147:1216	Decreased dietary crude protein levels in growing and finishing period had insignificant effects on chicken's performance (P > 0.05).					
35124796	5	45	contain	had	1218:1220	arg2	effects					1236:1242	insignificant effects	1222:1242	insignificant effects	1222:1242	Decreased dietary crude protein levels in growing and finishing period had insignificant effects on chicken's performance (P > 0.05).					
35124796	3	46	theme	nutrient	957:964	arg1	P < 0.05					974:981	P < 0.05	974:981	P < 0.05	974:981	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	3	46	theme	nutrient	957:964	arg1	levels					966:971	fecal nutrient levels	951:971	fecal nutrient levels (P < 0.05)	951:982	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	7	47	theme	protein	1513:1519	arg1	level					1521:1525	Lower crude protein level	1501:1525	Lower crude protein level	1501:1525	Lower crude protein level changed the intestinal morphology (P < 0.05).					
35124796	9	48	theme	protein	1769:1775	arg1	levels					1777:1782	normal crude protein levels	1756:1782	normal crude protein levels	1756:1782	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	5	49	from	levels	1179:1184	arg1	period					1211:1216	growing and finishing period	1189:1216	growing and finishing period	1189:1216	Decreased dietary crude protein levels in growing and finishing period had insignificant effects on chicken's performance (P > 0.05).					
35124796	2	50	from	1	543:543	arg1	broilers					529:536	388 Ross-308 strain broilers	509:536	388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days)	509:671	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	4	51	theme	feed	1090:1093	arg1	ratio					1106:1110	feed conversion ratio	1090:1110	feed conversion ratio in the starting period (P < 0.05)	1090:1144	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	1	52	theme	protein	435:441	arg1	level					443:447	crude protein level	429:447	crude protein level	429:447	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	0	53	theme	Thyme	42:46	arg1	Oil					76:78	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil	0:78	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.	0:160	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	4	54	from	decrease	991:998	arg1	level					1017:1021	crude protein level	1003:1021	crude protein level of diet	1003:1029	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	2	55	dep	42 days	664:670	arg1	to					661:662	to	661:662	to	661:662	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	2	56	from	42 days	548:554	arg1	periods					578:584	three experimental periods	559:584	three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days)	559:671	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	2	56	from	42 days	548:554	arg1	finisher					648:655	finisher	648:655	finisher (25 to 42 days)	648:671	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	2	56	from	42 days	548:554	arg1	grower					620:625	grower	620:625	grower (11 to 24 days)	620:641	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	2	56	from	42 days	548:554	arg1	starter					596:602	starter	596:602	starter (1 to 10 days)	596:617	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	4	57	theme	weight	1059:1064	arg1	gain					1066:1069	daily weight gain	1053:1069	daily weight gain	1053:1069	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	5	58	theme	growing	1189:1195	arg1	period					1211:1216	growing and finishing period	1189:1216	growing and finishing period	1189:1216	Decreased dietary crude protein levels in growing and finishing period had insignificant effects on chicken's performance (P > 0.05).					
35124796	5	59	theme	protein	1171:1177	arg1	levels					1179:1184	Decreased dietary crude protein levels	1147:1184	Decreased dietary crude protein levels in growing and finishing period	1147:1216	Decreased dietary crude protein levels in growing and finishing period had insignificant effects on chicken's performance (P > 0.05).					
35124796	5	60	theme	Decreased	1147:1155	arg1	levels					1179:1184	Decreased dietary crude protein levels	1147:1184	Decreased dietary crude protein levels in growing and finishing period	1147:1216	Decreased dietary crude protein levels in growing and finishing period had insignificant effects on chicken's performance (P > 0.05).					
35124796	5	61	theme	crude	1165:1169	arg1	levels					1179:1184	Decreased dietary crude protein levels	1147:1184	Decreased dietary crude protein levels in growing and finishing period	1147:1216	Decreased dietary crude protein levels in growing and finishing period had insignificant effects on chicken's performance (P > 0.05).					
35124796	1	62	theme	natural	228:234	arg1	additives					241:249	selected natural feed additives	219:249	selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic)	219:305	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	62	theme	natural	228:234	arg1	extract					258:264	thyme extract	252:264	thyme extract	252:264	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	62	theme	natural	228:234	arg1	acid					275:278	organic acid	267:278	organic acid	267:278	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	62	theme	natural	228:234	arg1	prebiotic					296:304	prebiotic	296:304	prebiotic	296:304	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	7	63	theme	intestinal	1539:1548	arg1	P < 0.05					1562:1569	P < 0.05	1562:1569	P < 0.05	1562:1569	Lower crude protein level changed the intestinal morphology (P < 0.05).					
35124796	7	63	theme	intestinal	1539:1548	arg1	morphology					1550:1559	the intestinal morphology	1535:1559	the intestinal morphology (P < 0.05)	1535:1570	Lower crude protein level changed the intestinal morphology (P < 0.05).					
35124796	9	64	theme	feces	1842:1846	arg1	morphology					1807:1816	intestinal morphology	1796:1816	intestinal morphology	1796:1816	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	9	64	theme	feces	1842:1846	arg1	content					1831:1837	nutrient content	1822:1837	nutrient content	1822:1837	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	1	65	theme	protein	396:402	arg1	levels					404:409	two crude protein levels	386:409	two crude protein levels diet	386:414	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	66	theme	additives	241:249	arg1	effects					208:214	the effects	204:214	the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients	204:379	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	6	67	contain	had	1390:1392	arg2	effect					1397:1402	no effect	1394:1402	no effect	1394:1402	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	6	67	contain	had	1390:1392	arg1	use					1315:1317	the use	1311:1317	the use of feed additives on diets with lower than normal crude protein levels	1311:1388	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	2	68	theme	Ross-308	513:520	arg1	broilers					529:536	388 Ross-308 strain broilers	509:536	388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days)	509:671	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	3	69	theme	additives	834:842	arg1	use					822:824	use	822:824	use of feed additives along with both levels of crude protein	822:882	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	6	70	theme	feed	1322:1325	arg1	additives					1327:1335	feed additives	1322:1335	feed additives	1322:1335	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	3	71	theme	crude	870:874	arg1	protein					876:882	crude protein	870:882	crude protein	870:882	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	5	72	theme	finishing	1201:1209	arg1	period					1211:1216	growing and finishing period	1189:1216	growing and finishing period	1189:1216	Decreased dietary crude protein levels in growing and finishing period had insignificant effects on chicken's performance (P > 0.05).					
35124796	7	73	theme	Lower	1501:1505	arg1	level					1521:1525	Lower crude protein level	1501:1525	Lower crude protein level	1501:1525	Lower crude protein level changed the intestinal morphology (P < 0.05).					
35124796	8	74	theme	feces	1650:1654	arg1	content					1639:1645	the nutrient content	1626:1645	the nutrient content of feces (P < 0.05)	1626:1665	The use of feed additives had significant effects on the nutrient content of feces (P < 0.05).					
35124796	2	75	used	used	678:681	arg2	broilers					529:536	388 Ross-308 strain broilers	509:536	388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days)	509:671	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	6	76	theme	feed	1455:1458	arg1	ratio					1471:1475	feed conversion ratio	1455:1475	feed conversion ratio	1455:1475	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	6	77	theme	experiment	1292:1301	arg1	period					1303:1308	the experiment period	1288:1308	the experiment period	1288:1308	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	2	78	theme	factorial	771:779	arg1	arrangements					781:792	5 × 2 factorial arrangements	765:792	5 × 2 factorial arrangements	765:792	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	6	79	theme	crude	1369:1373	arg1	levels					1383:1388	lower than normal crude protein levels	1351:1388	lower than normal crude protein levels	1351:1388	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	3	80	from	effects	900:906	arg1	performance					911:921	performance	911:921	performance	911:921	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	6	81	theme	weight	1438:1443	arg1	gain					1445:1448	daily weight gain	1432:1448	daily weight gain	1432:1448	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	9	82	theme	intestinal	1796:1805	arg1	morphology					1807:1816	intestinal morphology	1796:1816	intestinal morphology	1796:1816	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	4	83	theme	protein	1009:1015	arg1	level					1017:1021	crude protein level	1003:1021	crude protein level of diet	1003:1029	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	0	84	theme	Nutrient	140:147	arg1	Composition					149:159	Fecal Nutrient Composition	134:159	Fecal Nutrient Composition	134:159	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	1	85	theme	nutrients	371:379	arg1	composition					350:360	composition	350:360	composition	350:360	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	85	theme	nutrients	371:379	arg1	performance					310:320	performance	310:320	performance	310:320	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	85	theme	nutrients	371:379	arg1	morphology					334:343	intestinal morphology	323:343	intestinal morphology	323:343	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	6	86	theme	average	1411:1417	arg1	intake					1424:1429	the average feed intake	1407:1429	the average feed intake	1407:1429	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	9	87	theme	protein	1722:1728	arg1	level					1730:1734	crude protein level	1716:1734	crude protein level of diet	1716:1742	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	3	88	from	levels	966:971	arg1	performance					911:921	performance	911:921	performance	911:921	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	9	89	theme	10	1699:1700	arg1	%					1701:1701	%	1701:1701	%	1701:1701	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	4	90	theme	%	989:989	arg1	decrease					991:998	A 10% decrease	985:998	A 10% decrease in crude protein level of diet	985:1029	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	0	91	theme	Essential	66:74	arg1	Oil					76:78	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil	0:78	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.	0:160	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	9	92	dep	morphology	1807:1816	arg1	the					1792:1794	the	1792:1794	the	1792:1794	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	9	93	theme	crude	1716:1720	arg1	level					1730:1734	crude protein level	1716:1734	crude protein level of diet	1716:1742	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	6	94	with	diets	1340:1344	arg1	levels					1383:1388	lower than normal crude protein levels	1351:1388	lower than normal crude protein levels	1351:1388	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	2	95	theme	experimental	565:576	arg1	periods					578:584	three experimental periods	559:584	three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days)	559:671	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	2	95	theme	experimental	565:576	arg1	finisher					648:655	finisher	648:655	finisher (25 to 42 days)	648:671	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	2	95	theme	experimental	565:576	arg1	grower					620:625	grower	620:625	grower (11 to 24 days)	620:641	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	2	95	theme	experimental	565:576	arg1	starter					596:602	starter	596:602	starter (1 to 10 days)	596:617	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	0	96	theme	Fecal	134:138	arg1	Composition					149:159	Fecal Nutrient Composition	134:159	Fecal Nutrient Composition	134:159	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	6	97	theme	chickens	1480:1487	arg1	intake					1424:1429	the average feed intake	1407:1429	the average feed intake	1407:1429	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	6	97	theme	chickens	1480:1487	arg1	ratio					1471:1475	feed conversion ratio	1455:1475	feed conversion ratio	1455:1475	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	6	97	theme	chickens	1480:1487	arg1	gain					1445:1448	daily weight gain	1432:1448	daily weight gain	1432:1448	During the experiment period, the use of feed additives on diets with lower than normal crude protein levels had no effect on the average feed intake, daily weight gain, and feed conversion ratio of chickens (P > 0.05).					
35124796	4	98	theme	daily	1053:1057	arg1	gain					1066:1069	daily weight gain	1053:1069	daily weight gain	1053:1069	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	3	99	theme	intestinal	924:933	arg1	morphology					935:944	intestinal morphology	924:944	intestinal morphology	924:944	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	4	100	from	decrease	1041:1048	arg1	ratio					1106:1110	feed conversion ratio	1090:1110	feed conversion ratio in the starting period (P < 0.05)	1090:1144	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	4	100	from	decrease	1041:1048	arg1	gain					1066:1069	daily weight gain	1053:1069	daily weight gain	1053:1069	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	9	101	from	reduction	1703:1711	arg1	level					1730:1734	crude protein level	1716:1734	crude protein level of diet	1716:1742	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	9	102	theme	diet	1739:1742	arg1	level					1730:1734	crude protein level	1716:1734	crude protein level of diet	1716:1742	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	0	103	theme	Prebiotics	14:23	arg1	Oil					76:78	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil	0:78	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.	0:160	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	2	104	theme	5 × 2	765:769	arg1	arrangements					781:792	5 × 2 factorial arrangements	765:792	5 × 2 factorial arrangements	765:792	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	4	105	from	ratio	1106:1110	arg1	P < 0.05					1136:1143	P < 0.05	1136:1143	P < 0.05	1136:1143	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	4	105	from	ratio	1106:1110	arg1	period					1128:1133	the starting period	1115:1133	the starting period (P < 0.05)	1115:1144	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	0	106	theme	Probiotics	26:35	arg1	Oil					76:78	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil	0:78	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.	0:160	Supplementary Prebiotics, Probiotics, and Thyme (Thymus vulgaris) Essential Oil for Broilers: Performance, Intestinal Morphology, and Fecal Nutrient Composition.					
35124796	3	107	theme	fecal	951:955	arg1	P < 0.05					974:981	P < 0.05	974:981	P < 0.05	974:981	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	3	107	theme	fecal	951:955	arg1	levels					966:971	fecal nutrient levels	951:971	fecal nutrient levels (P < 0.05)	951:982	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	2	108	dep	10 days	610:616	arg1	to					607:608	to	607:608	to	607:608	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	1	109	theme	recommended	457:467	arg1	level					469:473	the recommended level	453:473	the recommended level	453:473	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	2	110	dep	replicates	734:743	arg1	treatments					720:729	8 treatments	718:729	8 treatments	718:729	In this experiment, 388 Ross-308 strain broilers from 1 to 42 days in three experimental periods including starter (1 to 10 days), grower (11 to 24 days), and finisher (25 to 42 days) were used in a completely randomized design (8 treatments, 4 replicates, and 12 chickens in 5 × 2 factorial arrangements).					
35124796	1	111	theme	organic	267:273	arg1	additives					241:249	selected natural feed additives	219:249	selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic)	219:305	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	111	theme	organic	267:273	arg1	acid					275:278	organic acid	267:278	organic acid	267:278	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	4	112	from	increase	1078:1085	arg1	ratio					1106:1110	feed conversion ratio	1090:1110	feed conversion ratio in the starting period (P < 0.05)	1090:1144	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	4	112	from	increase	1078:1085	arg1	gain					1066:1069	daily weight gain	1053:1069	daily weight gain	1053:1069	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	4	113	theme	conversion	1095:1104	arg1	ratio					1106:1110	feed conversion ratio	1090:1110	feed conversion ratio in the starting period (P < 0.05)	1090:1144	A 10% decrease in crude protein level of diet caused to decrease in daily weight gain and an increase in feed conversion ratio in the starting period (P < 0.05).					
35124796	1	114	theme	crude	429:433	arg1	level					443:447	crude protein level	429:447	crude protein level	429:447	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	3	115	theme	feed	829:832	arg1	additives					834:842	feed additives	829:842	feed additives	829:842	According to the results, use of feed additives along with both levels of crude protein had significant effects on performance, intestinal morphology, and fecal nutrient levels (P < 0.05).					
35124796	9	116	theme	nutrient	1822:1829	arg1	content					1831:1837	nutrient content	1822:1837	nutrient content	1822:1837	Overall, results showed that a 10% reduction in crude protein level of diet compared to normal crude protein levels changed the intestinal morphology and nutrient content of feces while having adverse effects on the performance of chickens.					
35124796	5	117	theme	dietary	1157:1163	arg1	levels					1179:1184	Decreased dietary crude protein levels	1147:1184	Decreased dietary crude protein levels in growing and finishing period	1147:1216	Decreased dietary crude protein levels in growing and finishing period had insignificant effects on chicken's performance (P > 0.05).					
35124796	1	118	theme	selected	219:226	arg1	additives					241:249	selected natural feed additives	219:249	selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic)	219:305	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	118	theme	selected	219:226	arg1	extract					258:264	thyme extract	252:264	thyme extract	252:264	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	118	theme	selected	219:226	arg1	acid					275:278	organic acid	267:278	organic acid	267:278	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
35124796	1	118	theme	selected	219:226	arg1	prebiotic					296:304	prebiotic	296:304	prebiotic	296:304	This experiment was performed to evaluate the effects of selected natural feed additives (thyme extract, organic acid, probiotic, and prebiotic) on performance, intestinal morphology, and composition of fecal nutrients with two crude protein levels diet (10% reduced crude protein level and the recommended level) in broilers.					
36441438	7	0	theme	co-products	1287:1297	arg1	retention					1323:1331	the co-products increased (P < 0.001) N retention	1283:1331	the co-products increased (P < 0.001) N retention	1283:1331	The additional N supplied by the co-products increased (P < 0.001) N retention, yields of milk, fat, protein, and lactose.					
36441438	6	1	theme	total	1143:1147	arg1	DM					1161:1162	DM	1161:1162	DM; P < 0.001	1161:1173	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	6	1	theme	total	1143:1147	arg1	matter					1153:1158	total dry matter	1143:1158	total dry matter (DM; P < 0.001)	1143:1174	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	4	2	theme	maize	794:798	arg1	cake					804:807	maize oil cake	794:807	maize oil cake	794:807	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	1	3	theme	milk	265:268	arg1	constituents					279:290	milk and milk constituents	265:290	constituents	279:290	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	9	4	theme	health	1827:1832	arg1	C18:1n-9					1845:1852	health beneficial C18:1n-9	1827:1852	health beneficial C18:1n-9	1827:1852	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	1	5	theme	co-product	174:183	arg1	supplementations					185:200	different oilseed co-product supplementations	156:200	different oilseed co-product supplementations	156:200	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	4	6	theme	seed	780:783	arg1	cake					785:788	mustard seed cake	772:788	mustard seed cake	772:788	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	10	7	theme	dairy	2015:2019	arg1	rations					2026:2032	dairy goat rations	2015:2032	dairy goat rations	2015:2032	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	9	8	theme	C18:1n-9	1845:1852	arg1	contents					1815:1822	the contents	1811:1822	the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat	1811:1945	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	8	9	theme	maize	1611:1615	arg1	cake					1621:1624	maize oil cake	1611:1624	maize oil cake	1611:1624	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	2	10	theme	block	527:531	arg1	design					533:538	randomized complete block design	507:538	randomized complete block design	507:538	In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk) were assigned to four experimental rations according to randomized complete block design.					
36441438	5	11	dep	BW	883:884	arg1	milk/day					910:917	producing 1250 ± 110 g milk/day	887:917	44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day	871:917	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	9	12	theme	C18:1	1855:1859	arg1	contents					1815:1822	the contents	1811:1822	the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat	1811:1945	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	2	13	theme	randomized	507:516	arg1	design					533:538	randomized complete block design	507:538	randomized complete block design	507:538	In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk) were assigned to four experimental rations according to randomized complete block design.					
36441438	5	14	theme	metabolism	958:967	arg1	crates					969:974	individual metabolism crates	947:974	individual metabolism crates for a digestibility and N balance experiment	947:1019	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	3	15	theme	daily	570:574	arg1	yield					581:585	daily milk yield	570:585	daily milk yield	570:585	The blocks were balanced for daily milk yield, parity, and body weight.					
36441438	1	16	theme	nutrient	218:225	arg1	digestibility					227:239	nutrient digestibility	218:239	nutrient digestibility	218:239	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	10	17	theme	goat	2021:2024	arg1	rations					2026:2032	dairy goat rations	2015:2032	dairy goat rations	2015:2032	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	8	18	dep	greatest	1463:1470	arg1	P < 0.05					1473:1480	P < 0.05	1473:1480	P < 0.05	1473:1480	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	10	19	theme	maize	2189:2193	arg1	cake					2199:2202	maize oil cake	2189:2202	maize oil cake	2189:2202	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	8	20	theme	milk	1523:1526	arg1	11.6 g/day					1537:1546	11.6 g/day	1537:1546	11.6 g/day	1537:1546	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	8	20	theme	milk	1523:1526	arg1	protein					1528:1534	milk protein	1523:1534	milk protein (11.6 g/day)	1523:1547	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	4	21	theme	straw-based	664:674	arg1	libitum					692:698	a maize silage and wheat straw-based basal ration ad libitum	639:698	libitum	692:698	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	6	22	theme	matter	1153:1158	arg1	intakes					1104:1110	intakes	1104:1110	intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001)	1104:1251	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	5	23	theme	N	1000:1000	arg1	balance					1002:1008	N balance	1000:1008	N balance	1000:1008	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	10	24	theme	co-product	1991:2000	arg1	inclusion					2002:2010	the oilseed co-product inclusion	1979:2010	the oilseed co-product inclusion in dairy goat rations	1979:2032	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	4	25	theme	maize	641:645	arg1	control					701:707	control	701:707	control	701:707	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	4	25	theme	maize	641:645	arg1	silage					647:652	a maize silage and wheat straw-based basal ration ad libitum	639:698	silage	647:652	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	0	26	theme	fatty	80:84	arg1	acids					86:90	fatty acids	80:90	fatty acids	80:90	Effect of supplementation of oilseeds co-products on production performance and fatty acids composition of Beetal goats.					
36441438	8	27	theme	fats	1550:1553	arg1	yields					1495:1500	yields	1495:1500	yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day)	1495:1592	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	1	28	theme	different	156:164	arg1	supplementations					185:200	different oilseed co-product supplementations	156:200	different oilseed co-product supplementations	156:200	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	9	29	theme	novo-synthesized	1747:1762	arg1	FAs					1774:1776	novo-synthesized saturated FAs	1747:1776	all de novo-synthesized saturated FAs (SFAs)	1740:1783	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	9	29	theme	novo-synthesized	1747:1762	arg1	SFAs					1779:1782	SFAs	1779:1782	SFAs	1779:1782	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	5	30	theme	lactation	842:850	arg1	trial					852:856	lactation trial	842:856	lactation trial	842:856	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	9	31	theme	total	1913:1917	arg1	FAs					1931:1933	total unsaturated FAs	1913:1933	total unsaturated FAs	1913:1933	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	7	32	theme	milk	1344:1347	arg1	retention					1323:1331	the co-products increased (P < 0.001) N retention	1283:1331	the co-products increased (P < 0.001) N retention	1283:1331	The additional N supplied by the co-products increased (P < 0.001) N retention, yields of milk, fat, protein, and lactose.					
36441438	7	32	theme	milk	1344:1347	arg1	lactose					1368:1374	lactose	1368:1374	lactose	1368:1374	The additional N supplied by the co-products increased (P < 0.001) N retention, yields of milk, fat, protein, and lactose.					
36441438	7	32	theme	milk	1344:1347	arg1	fat					1350:1352	fat	1350:1352	fat	1350:1352	The additional N supplied by the co-products increased (P < 0.001) N retention, yields of milk, fat, protein, and lactose.					
36441438	7	32	theme	milk	1344:1347	arg1	protein					1355:1361	protein	1355:1361	protein	1355:1361	The additional N supplied by the co-products increased (P < 0.001) N retention, yields of milk, fat, protein, and lactose.					
36441438	7	32	theme	milk	1344:1347	arg1	yields					1334:1339	yields	1334:1339	yields of milk	1334:1347	The additional N supplied by the co-products increased (P < 0.001) N retention, yields of milk, fat, protein, and lactose.					
36441438	2	33	theme	experimental	473:484	arg1	rations					486:492	four experimental rations	468:492	four experimental rations	468:492	In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk) were assigned to four experimental rations according to randomized complete block design.					
36441438	9	34	theme	FAs	1931:1933	arg1	contents					1815:1822	the contents	1811:1822	the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat	1811:1945	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	0	35	theme	Beetal	107:112	arg1	goats					114:118	Beetal goats	107:118	Beetal goats	107:118	Effect of supplementation of oilseeds co-products on production performance and fatty acids composition of Beetal goats.					
36441438	1	36	theme	goats	336:340	arg1	intake					210:215	feed intake	205:215	feed intake	205:215	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	36	theme	goats	336:340	arg1	digestibility					227:239	nutrient digestibility	218:239	nutrient digestibility	218:239	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	36	theme	goats	336:340	arg1	profile					318:324	milk fatty acid (FA) profile	297:324	milk fatty acid (FA) profile of Beetal goats	297:340	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	36	theme	goats	336:340	arg1	retention					244:252	N retention	242:252	N retention	242:252	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	36	theme	goats	336:340	arg1	yields					255:260	yields	255:260	yields of milk and milk constituents	255:290	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	10	37	theme	milk	2104:2107	arg1	constituents					2109:2120	milk and milk constituents	2095:2120	constituents	2109:2120	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	4	38	theme	ration	682:687	arg1	libitum					692:698	a maize silage and wheat straw-based basal ration ad libitum	639:698	libitum	692:698	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	9	39	theme	milk	1938:1941	arg1	fat					1943:1945	milk fat	1938:1945	milk fat	1938:1945	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	8	40	theme	lactose	1573:1579	arg1	yields					1495:1500	yields	1495:1500	yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day)	1495:1592	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	10	41	theme	milk	2095:2098	arg1	constituents					2109:2120	milk and milk constituents	2095:2120	constituents	2109:2120	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	2	42	theme	multiparous	388:398	arg1	goats					407:411	thirty-six lactating multiparous Beetal goats	367:411	thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk)	367:449	In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk) were assigned to four experimental rations according to randomized complete block design.					
36441438	0	43	theme	supplementation	10:24	arg1	Effect					0:5	Effect	0:5	Effect of supplementation of oilseeds	0:36	Effect of supplementation of oilseeds co-products on production performance and fatty acids composition of Beetal goats.					
36441438	1	44	theme	constituents	279:290	arg1	intake					210:215	feed intake	205:215	feed intake	205:215	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	44	theme	constituents	279:290	arg1	digestibility					227:239	nutrient digestibility	218:239	nutrient digestibility	218:239	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	44	theme	constituents	279:290	arg1	profile					318:324	milk fatty acid (FA) profile	297:324	milk fatty acid (FA) profile of Beetal goats	297:340	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	44	theme	constituents	279:290	arg1	retention					244:252	N retention	242:252	N retention	242:252	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	44	theme	constituents	279:290	arg1	yields					255:260	yields	255:260	yields of milk and milk constituents	255:290	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	0	45	dep	performance	64:74	arg1	composition					92:102	composition	92:102	composition	92:102	Effect of supplementation of oilseeds co-products on production performance and fatty acids composition of Beetal goats.					
36441438	10	46	theme	milk	2127:2130	arg1	profile					2137:2143	milk FAs' profile	2127:2143	milk FAs' profile	2127:2143	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	9	47	theme	saturated	1764:1772	arg1	FAs					1774:1776	novo-synthesized saturated FAs	1747:1776	all de novo-synthesized saturated FAs (SFAs)	1740:1783	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	9	47	theme	saturated	1764:1772	arg1	SFAs					1779:1782	SFAs	1779:1782	SFAs	1779:1782	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	2	48	theme	thirty-six	367:376	arg1	goats					407:411	thirty-six lactating multiparous Beetal goats	367:411	thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk)	367:449	In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk) were assigned to four experimental rations according to randomized complete block design.					
36441438	0	49	theme	oilseeds	29:36	arg1	supplementation					10:24	supplementation	10:24	supplementation of oilseeds	10:36	Effect of supplementation of oilseeds co-products on production performance and fatty acids composition of Beetal goats.					
36441438	6	50	theme	protein	1229:1235	arg1	intakes					1104:1110	intakes	1104:1110	intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001)	1104:1251	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	7	51	theme	N	1321:1321	arg1	retention					1323:1331	the co-products increased (P < 0.001) N retention	1283:1331	the co-products increased (P < 0.001) N retention	1283:1331	The additional N supplied by the co-products increased (P < 0.001) N retention, yields of milk, fat, protein, and lactose.					
36441438	5	52	theme	1250 ± 110 g	897:908	arg1	milk/day					910:917	producing 1250 ± 110 g milk/day	887:917	44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day	871:917	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	4	53	theme	seed	761:764	arg1	cake					766:769	cotton seed cake	754:769	cotton seed cake	754:769	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	5	54	theme	Latin	1030:1034	arg1	4 × 4					1051:1055	4 × 4	1051:1055	4 × 4	1051:1055	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	5	54	theme	Latin	1030:1034	arg1	design					1043:1048	a Latin square design	1028:1048	a Latin square design (4 × 4)	1028:1056	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	6	55	dep	CP	1238:1239	arg1	P < 0.001					1242:1250	P < 0.001	1242:1250	CP; P < 0.001	1238:1250	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	4	56	theme	control	717:723	arg1	ration					725:730	the control ration	713:730	the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake	713:807	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	7	57	theme	increased	1299:1307	arg1	retention					1323:1331	the co-products increased (P < 0.001) N retention	1283:1331	the co-products increased (P < 0.001) N retention	1283:1331	The additional N supplied by the co-products increased (P < 0.001) N retention, yields of milk, fat, protein, and lactose.					
36441438	5	58	theme	44 ± 0.8 kg	871:881	arg1	BW					883:884	44 ± 0.8 kg BW	871:884	44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day	871:917	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	5	58	theme	44 ± 0.8 kg	871:881	arg1	goats					864:868	four goats	859:868	four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day)	859:918	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	1	59	theme	N	242:242	arg1	retention					244:252	N retention	242:252	N retention	242:252	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	6	60	theme	crude	1223:1227	arg1	protein					1229:1235	crude protein	1223:1235	crude protein (CP; P < 0.001)	1223:1251	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	6	60	theme	crude	1223:1227	arg1	CP					1238:1239	CP	1238:1239	CP; P < 0.001	1238:1250	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	8	61	theme	Further	1377:1383	arg1	comparison					1385:1394	Further comparison	1377:1394	Further comparison of the rations supplemented with the co-products	1377:1443	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	6	62	theme	dry	1149:1151	arg1	DM					1161:1162	DM	1161:1162	DM; P < 0.001	1161:1173	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	6	62	theme	dry	1149:1151	arg1	matter					1153:1158	total dry matter	1143:1158	total dry matter (DM; P < 0.001)	1143:1174	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	7	63	dep	increased	1299:1307	arg1	P < 0.001					1310:1318	P < 0.001	1310:1318	P < 0.001	1310:1318	The additional N supplied by the co-products increased (P < 0.001) N retention, yields of milk, fat, protein, and lactose.					
36441438	8	64	theme	oil	1617:1619	arg1	cake					1621:1624	maize oil cake	1611:1624	maize oil cake	1611:1624	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	10	65	from	inclusion	2002:2010	arg1	rations					2026:2032	dairy goat rations	2015:2032	dairy goat rations	2015:2032	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	8	66	theme	greatest	1463:1470	arg1	increase					1483:1490	the greatest (P < 0.05) increase	1459:1490	the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day)	1459:1592	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	7	67	theme	additional	1258:1267	arg1	N					1269:1269	The additional N	1254:1269	The additional N	1254:1269	The additional N supplied by the co-products increased (P < 0.001) N retention, yields of milk, fat, protein, and lactose.					
36441438	8	68	theme	rations	1403:1409	arg1	comparison					1385:1394	Further comparison	1377:1394	Further comparison of the rations supplemented with the co-products	1377:1443	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	10	69	theme	oilseed	1983:1989	arg1	inclusion					2002:2010	the oilseed co-product inclusion	1979:2010	the oilseed co-product inclusion in dairy goat rations	1979:2032	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	1	70	theme	oilseed	166:172	arg1	supplementations					185:200	different oilseed co-product supplementations	156:200	different oilseed co-product supplementations	156:200	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	71	theme	fatty	302:306	arg1	FA					314:315	FA	314:315	FA	314:315	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	71	theme	fatty	302:306	arg1	acid					308:311	milk fatty acid	297:311	milk fatty acid (FA) profile of Beetal goats	297:340	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	9	72	theme	beneficial	1834:1843	arg1	C18:1n-9					1845:1852	health beneficial C18:1n-9	1827:1852	health beneficial C18:1n-9	1827:1852	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	6	73	theme	mixture	1122:1128	arg1	intakes					1104:1110	intakes	1104:1110	intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001)	1104:1251	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	1	74	theme	supplementations	185:200	arg1	effect					146:151	the effect	142:151	the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats	142:340	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	4	75	theme	mustard	772:778	arg1	cake					785:788	mustard seed cake	772:788	mustard seed cake	772:788	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	9	76	dep	decreased	1703:1711	arg1	P < 0.01					1714:1721	P < 0.01	1714:1721	P < 0.01	1714:1721	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	1	77	theme	feed	205:208	arg1	intake					210:215	feed intake	205:215	feed intake	205:215	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	6	78	dep	DM	1161:1162	arg1	P < 0.001					1165:1173	P < 0.001	1165:1173	DM; P < 0.001	1161:1173	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	4	79	theme	iso-N	815:819	arg1	basis					821:825	an iso-N basis	812:825	an iso-N basis	812:825	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	10	80	theme	largest	2155:2161	arg1	impact					2163:2168	the largest impact	2151:2168	the largest impact	2151:2168	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	5	81	theme	individual	947:956	arg1	crates					969:974	individual metabolism crates	947:974	individual metabolism crates for a digestibility and N balance experiment	947:1019	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	8	82	theme	milk	1505:1508	arg1	yields					1495:1500	yields	1495:1500	yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day)	1495:1592	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	9	83	dep	increased	1789:1797	arg1	P < 0.001					1800:1808	P < 0.001	1800:1808	P < 0.001	1800:1808	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	1	84	theme	acid	308:311	arg1	profile					318:324	milk fatty acid (FA) profile	297:324	milk fatty acid (FA) profile of Beetal goats	297:340	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	9	85	theme	C18:2n-6	1870:1877	arg1	contents					1815:1822	the contents	1811:1822	the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat	1811:1945	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	4	86	theme	oil	800:802	arg1	cake					804:807	maize oil cake	794:807	maize oil cake	794:807	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	6	87	theme	co-products	1082:1092	arg1	Supplementation					1059:1073	Supplementation	1059:1073	Supplementation of the co-products	1059:1092	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	9	88	theme	C18:3n-3	1880:1887	arg1	contents					1815:1822	the contents	1811:1822	the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat	1811:1945	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	9	89	dep	FAs	1774:1776	arg1	de					1744:1745	all de novo-synthesized saturated FAs (SFAs)	1740:1783	all de novo-synthesized saturated FAs (SFAs)	1740:1783	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	4	90	theme	wheat	658:662	arg1	libitum					692:698	a maize silage and wheat straw-based basal ration ad libitum	639:698	libitum	692:698	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	9	91	theme	C20:2n-6	1890:1897	arg1	contents					1815:1822	the contents	1811:1822	the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat	1811:1945	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	3	92	theme	milk	576:579	arg1	yield					581:585	daily milk yield	570:585	daily milk yield	570:585	The blocks were balanced for daily milk yield, parity, and body weight.					
36441438	8	93	theme	protein	1528:1534	arg1	yields					1495:1500	yields	1495:1500	yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day)	1495:1592	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	9	94	theme	co-products	1691:1701	arg1	supplementation					1668:1682	supplementation	1668:1682	supplementation of the co-products	1668:1701	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	9	95	theme	C20:5n3	1900:1906	arg1	contents					1815:1822	the contents	1811:1822	the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat	1811:1945	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	5	96	theme	balance	1002:1008	arg1	experiment					1010:1019	a digestibility and N balance experiment	980:1019	a digestibility and N balance experiment	980:1019	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	6	97	dep	fibre	1195:1199	arg1	NDF					1202:1204	NDF	1202:1204	NDF; P = 0.003	1202:1215	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	2	98	theme	complete	518:525	arg1	block					527:531	complete block	518:531	randomized complete block design	507:538	In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk) were assigned to four experimental rations according to randomized complete block design.					
36441438	10	99	theme	oil	2195:2197	arg1	cake					2199:2202	maize oil cake	2189:2202	maize oil cake	2189:2202	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	6	100	dep	NDF	1202:1204	arg1	P = 0.003					1207:1215	P = 0.003	1207:1215	P = 0.003	1207:1215	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	5	101	theme	trial	852:856	arg1	end					835:837	the end	831:837	the end of lactation trial	831:856	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	2	102	from	15 ± 2.3 days	428:440	arg1	milk					445:448	milk	445:448	milk	445:448	In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk) were assigned to four experimental rations according to randomized complete block design.					
36441438	9	103	theme	unsaturated	1919:1929	arg1	FAs					1931:1933	total unsaturated FAs	1913:1933	total unsaturated FAs	1913:1933	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	6	104	theme	fibre	1195:1199	arg1	intakes					1104:1110	intakes	1104:1110	intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001)	1104:1251	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	1	105	theme	Beetal	329:334	arg1	goats					336:340	Beetal goats	329:340	Beetal goats	329:340	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	2	106	dep	goats	407:411	arg1	45 ± 2.04 kg					414:425	45 ± 2.04 kg	414:425	45 ± 2.04 kg; 15 ± 2.3 days in milk	414:448	In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk) were assigned to four experimental rations according to randomized complete block design.					
36441438	1	107	from	effect	146:151	arg1	yields					255:260	yields	255:260	yields of milk and milk constituents	255:290	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	107	from	effect	146:151	arg1	digestibility					227:239	nutrient digestibility	218:239	nutrient digestibility	218:239	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	107	from	effect	146:151	arg1	retention					244:252	N retention	242:252	N retention	242:252	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	107	from	effect	146:151	arg1	profile					318:324	milk fatty acid (FA) profile	297:324	milk fatty acid (FA) profile of Beetal goats	297:340	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	107	from	effect	146:151	arg1	intake					210:215	feed intake	205:215	feed intake	205:215	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	10	108	theme	biomass	2064:2070	arg1	utilization					2072:2082	biomass utilization	2064:2082	biomass utilization	2064:2082	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	0	109	theme	goats	114:118	arg1	performance					64:74	production performance	53:74	production performance	53:74	Effect of supplementation of oilseeds co-products on production performance and fatty acids composition of Beetal goats.					
36441438	0	109	theme	goats	114:118	arg1	acids					86:90	fatty acids	80:90	fatty acids	80:90	Effect of supplementation of oilseeds co-products on production performance and fatty acids composition of Beetal goats.					
36441438	2	110	dep	45 ± 2.04 kg	414:425	arg1	15 ± 2.3 days					428:440	15 ± 2.3 days	428:440	45 ± 2.04 kg; 15 ± 2.3 days in milk	414:448	In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk) were assigned to four experimental rations according to randomized complete block design.					
36441438	4	111	theme	basal	676:680	arg1	libitum					692:698	a maize silage and wheat straw-based basal ration ad libitum	639:698	libitum	692:698	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	9	112	from	contents	1815:1822	arg1	fat					1943:1945	milk fat	1938:1945	milk fat	1938:1945	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	1	113	theme	milk	274:277	arg1	constituents					279:290	milk and milk constituents	265:290	constituents	279:290	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	2	114	theme	Beetal	400:405	arg1	goats					407:411	thirty-six lactating multiparous Beetal goats	367:411	thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk)	367:449	In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk) were assigned to four experimental rations according to randomized complete block design.					
36441438	3	115	theme	body	600:603	arg1	weight					605:610	body weight	600:610	body weight	600:610	The blocks were balanced for daily milk yield, parity, and body weight.					
36441438	4	116	theme	ad	689:690	arg1	libitum					692:698	a maize silage and wheat straw-based basal ration ad libitum	639:698	libitum	692:698	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	2	117	theme	lactating	378:386	arg1	goats					407:411	thirty-six lactating multiparous Beetal goats	367:411	thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk)	367:449	In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk) were assigned to four experimental rations according to randomized complete block design.					
36441438	5	118	theme	producing	887:895	arg1	milk/day					910:917	producing 1250 ± 110 g milk/day	887:917	44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day	871:917	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	5	119	dep	digestibility	982:994	arg1	a					980:980	a	980:980	a	980:980	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	9	120	theme	FAs	1774:1776	arg1	contents					1728:1735	the contents	1724:1735	the contents of all de novo-synthesized saturated FAs (SFAs)	1724:1783	Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat.					
36441438	8	121	from	increase	1483:1490	arg1	yields					1495:1500	yields	1495:1500	yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day)	1495:1592	Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240 g/day), milk protein (11.6 g/day), fats (16.3 g/day), and lactose (11.2 g/day) was recorded for maize oil cake, as compared to the control.					
36441438	0	122	theme	production	53:62	arg1	performance					64:74	production performance	53:74	production performance	53:74	Effect of supplementation of oilseeds co-products on production performance and fatty acids composition of Beetal goats.					
36441438	1	123	theme	milk	297:300	arg1	FA					314:315	FA	314:315	FA	314:315	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	1	123	theme	milk	297:300	arg1	acid					308:311	milk fatty acid	297:311	milk fatty acid (FA) profile of Beetal goats	297:340	This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats.					
36441438	5	124	theme	digestibility	982:994	arg1	experiment					1010:1019	a digestibility and N balance experiment	980:1019	a digestibility and N balance experiment	980:1019	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	10	125	theme	constituents	2109:2120	arg1	profile					2137:2143	milk FAs' profile	2127:2143	milk FAs' profile	2127:2143	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	10	125	theme	constituents	2109:2120	arg1	utilization					2072:2082	biomass utilization	2064:2082	biomass utilization	2064:2082	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	10	125	theme	constituents	2109:2120	arg1	yields					2085:2090	yields	2085:2090	yields of milk and milk constituents	2085:2120	Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.					
36441438	4	126	theme	cotton	754:759	arg1	cake					766:769	cotton seed cake	754:769	cotton seed cake	754:769	The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis.					
36441438	5	127	theme	square	1036:1041	arg1	4 × 4					1051:1055	4 × 4	1051:1055	4 × 4	1051:1055	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	5	127	theme	square	1036:1041	arg1	design					1043:1048	a Latin square design	1028:1048	a Latin square design (4 × 4)	1028:1056	At the end of lactation trial, four goats (44 ± 0.8 kg BW; producing 1250 ± 110 g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4).					
36441438	6	128	dep	mixture	1122:1128	arg1	forage					1115:1120	forage	1115:1120	forage	1115:1120	Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001).					
36441438	2	129	theme	lactation	350:358	arg1	trial					360:364	the lactation trial	346:364	the lactation trial	346:364	In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04 kg; 15 ± 2.3 days in milk) were assigned to four experimental rations according to randomized complete block design.					
36746592	0	0	theme	gas	94:96	arg1	barrier					98:104	excellence gas barrier	83:104	excellence gas barrier	83:104	High strength, controlled release of curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier and antibacterial activity for litchi preservation.					
36746592	0	1	theme	excellence	83:92	arg1	barrier					98:104	excellence gas barrier	83:104	excellence gas barrier	83:104	High strength, controlled release of curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier and antibacterial activity for litchi preservation.					
36746592	1	2	theme	protein	190:196	arg1	additives					198:206	protein additives	190:206	protein additives	190:206	Polysaccharide films containing protein additives have good application prospects in agriculture and food field.					
36746592	8	3	theme	food	1407:1410	arg1	field					1412:1416	food field	1407:1416	food field	1407:1416	Therefore, this study provides a novel method to improve the interfacial compatibility between hydrophobic proteins and hydrophilic polymers, and is expected to expand the application of protein/polymer composites in agriculture and food field.					
36746592	6	4	used	used	985:988	arg2	Cur-ZIF-8/CS/Zein					955:971	Cur-ZIF-8/CS/Zein	955:971	Cur-ZIF-8/CS/Zein	955:971	Importantly, Cur-ZIF-8/CS/Zein can also be used as an intelligent-responsive release platform for curcumin.					
36746592	6	4	used	used	985:988	arg2	platform					1027:1034	an intelligent-responsive release platform	993:1034	an intelligent-responsive release platform for curcumin	993:1047	Importantly, Cur-ZIF-8/CS/Zein can also be used as an intelligent-responsive release platform for curcumin.					
36746592	3	5	theme	hydrophobic	451:461	arg1	zein					463:466	hydrophobic zein	451:466	hydrophobic zein	451:466	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	3	6	theme	zeolitic	576:583	arg1	Cur-ZIF-8					610:618	Cur-ZIF-8	610:618	Cur-ZIF-8	610:618	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	3	6	theme	zeolitic	576:583	arg1	framework-8					597:607	curcumin-loaded zeolitic imidazolate framework-8	560:607	curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8)	560:619	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	2	7	theme	hydrophilic	341:351	arg1	polymers					353:360	hydrophilic polymers	341:360	hydrophilic polymers	341:360	However, interfacial incompatibility between hydrophobic proteins and hydrophilic polymers remains a major technical challenge.					
36746592	4	8	from	break	728:732	arg1	elongation					714:723	the elongation	710:723	the elongation at break	710:732	With the improvement of interface compatibility, the results show that the elongation at break and O2 barrier property of synthesized Cur-ZIF-8/CS/Zein are 9.2 and 1.5 times higher than CS/Zein, respectively.					
36746592	4	8	from	break	728:732	arg1	9.2					795:797	9.2	795:797	9.2	795:797	With the improvement of interface compatibility, the results show that the elongation at break and O2 barrier property of synthesized Cur-ZIF-8/CS/Zein are 9.2 and 1.5 times higher than CS/Zein, respectively.					
36746592	4	8	from	break	728:732	arg1	property					749:756	O2 barrier property	738:756	O2 barrier property of synthesized Cur-ZIF-8/CS/Zein	738:789	With the improvement of interface compatibility, the results show that the elongation at break and O2 barrier property of synthesized Cur-ZIF-8/CS/Zein are 9.2 and 1.5 times higher than CS/Zein, respectively.					
36746592	4	9	theme	Cur-ZIF-8/CS/Zein	773:789	arg1	elongation					714:723	the elongation	710:723	the elongation at break	710:732	With the improvement of interface compatibility, the results show that the elongation at break and O2 barrier property of synthesized Cur-ZIF-8/CS/Zein are 9.2 and 1.5 times higher than CS/Zein, respectively.					
36746592	4	9	theme	Cur-ZIF-8/CS/Zein	773:789	arg1	9.2					795:797	9.2	795:797	9.2	795:797	With the improvement of interface compatibility, the results show that the elongation at break and O2 barrier property of synthesized Cur-ZIF-8/CS/Zein are 9.2 and 1.5 times higher than CS/Zein, respectively.					
36746592	4	9	theme	Cur-ZIF-8/CS/Zein	773:789	arg1	property					749:756	O2 barrier property	738:756	O2 barrier property of synthesized Cur-ZIF-8/CS/Zein	738:789	With the improvement of interface compatibility, the results show that the elongation at break and O2 barrier property of synthesized Cur-ZIF-8/CS/Zein are 9.2 and 1.5 times higher than CS/Zein, respectively.					
36746592	4	10	theme	barrier	741:747	arg1	property					749:756	O2 barrier property	738:756	O2 barrier property of synthesized Cur-ZIF-8/CS/Zein	738:789	With the improvement of interface compatibility, the results show that the elongation at break and O2 barrier property of synthesized Cur-ZIF-8/CS/Zein are 9.2 and 1.5 times higher than CS/Zein, respectively.					
36746592	1	11	contain	containing	179:188	arg1	films					173:177	Polysaccharide films	158:177	Polysaccharide films containing protein additives	158:206	Polysaccharide films containing protein additives have good application prospects in agriculture and food field.					
36746592	1	11	contain	containing	179:188	arg2	additives					198:206	protein additives	190:206	protein additives	190:206	Polysaccharide films containing protein additives have good application prospects in agriculture and food field.					
36746592	3	12	with	crosslinking	526:537	arg1	CS					626:627	CS	626:627	CS	626:627	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	3	12	with	crosslinking	526:537	arg1	zein					633:636	zein	633:636	zein	633:636	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	0	13	with	film	73:76	arg1	barrier					98:104	excellence gas barrier	83:104	excellence gas barrier	83:104	High strength, controlled release of curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier and antibacterial activity for litchi preservation.					
36746592	3	14	theme	framework-8	597:607	arg1	ions					552:555	zinc ions	547:555	zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8)	547:619	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	7	15	dep	freshness	1094:1102	arg1	the					1090:1092	the	1090:1092	the	1090:1092	As a result, Cur-ZIF-8/CS/Zein can keep the freshness and appearance of litchi at least 8 days longer than that of CS/Zein.					
36746592	7	15	dep	freshness	1094:1102	arg1	8 days					1138:1143	8 days	1138:1143	8 days	1138:1143	As a result, Cur-ZIF-8/CS/Zein can keep the freshness and appearance of litchi at least 8 days longer than that of CS/Zein.					
36746592	0	16	theme	antibacterial	110:122	arg1	activity					124:131	antibacterial activity	110:131	antibacterial activity for litchi preservation	110:155	High strength, controlled release of curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier and antibacterial activity for litchi preservation.					
36746592	2	17	theme	hydrophobic	316:326	arg1	proteins					328:335	hydrophobic proteins	316:335	hydrophobic proteins	316:335	However, interfacial incompatibility between hydrophobic proteins and hydrophilic polymers remains a major technical challenge.					
36746592	8	18	theme	hydrophobic	1269:1279	arg1	proteins					1281:1288	hydrophobic proteins	1269:1288	hydrophobic proteins	1269:1288	Therefore, this study provides a novel method to improve the interfacial compatibility between hydrophobic proteins and hydrophilic polymers, and is expected to expand the application of protein/polymer composites in agriculture and food field.					
36746592	8	19	theme	novel	1207:1211	arg1	method					1213:1218	a novel method	1205:1218	a novel method to improve the interfacial compatibility between hydrophobic proteins and hydrophilic polymers	1205:1313	Therefore, this study provides a novel method to improve the interfacial compatibility between hydrophobic proteins and hydrophilic polymers, and is expected to expand the application of protein/polymer composites in agriculture and food field.					
36746592	2	20	theme	major	372:376	arg1	challenge					388:396	a major technical challenge	370:396	a major technical challenge	370:396	However, interfacial incompatibility between hydrophobic proteins and hydrophilic polymers remains a major technical challenge.					
36746592	1	21	theme	good	213:216	arg1	prospects					230:238	good application prospects	213:238	good application prospects	213:238	Polysaccharide films containing protein additives have good application prospects in agriculture and food field.					
36746592	0	22	theme	High	0:3	arg1	strength					5:12	High strength	0:12	High strength	0:12	High strength, controlled release of curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier and antibacterial activity for litchi preservation.					
36746592	1	23	theme	application	218:228	arg1	prospects					230:238	good application prospects	213:238	good application prospects	213:238	Polysaccharide films containing protein additives have good application prospects in agriculture and food field.					
36746592	3	24	theme	interfacial	417:427	arg1	compatibility					429:441	the interfacial compatibility	413:441	the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS)	413:496	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	0	25	theme	litchi	137:142	arg1	preservation					144:155	litchi preservation	137:155	litchi preservation	137:155	High strength, controlled release of curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier and antibacterial activity for litchi preservation.					
36746592	5	26	theme	antibacterial	892:904	arg1	properties					922:931	superior antibacterial and antioxidant properties	883:931	superior antibacterial and antioxidant properties	883:931	And the Cur-ZIF-8/CS/Zein exhibits superior antibacterial and antioxidant properties as well.					
36746592	4	27	theme	interface	663:671	arg1	compatibility					673:685	interface compatibility	663:685	interface compatibility	663:685	With the improvement of interface compatibility, the results show that the elongation at break and O2 barrier property of synthesized Cur-ZIF-8/CS/Zein are 9.2 and 1.5 times higher than CS/Zein, respectively.					
36746592	3	28	theme	curcumin-loaded	560:574	arg1	Cur-ZIF-8					610:618	Cur-ZIF-8	610:618	Cur-ZIF-8	610:618	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	3	28	theme	curcumin-loaded	560:574	arg1	framework-8					597:607	curcumin-loaded zeolitic imidazolate framework-8	560:607	curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8)	560:619	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	3	29	theme	chemical	517:524	arg1	crosslinking					526:537	the chemical crosslinking	513:537	the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein	513:636	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	3	30	theme	hydrophilic	472:482	arg1	CS					494:495	CS	494:495	CS	494:495	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	3	30	theme	hydrophilic	472:482	arg1	chitosan					484:491	hydrophilic chitosan	472:491	hydrophilic chitosan (CS)	472:496	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	8	31	theme	protein/polymer	1361:1375	arg1	composites					1377:1386	protein/polymer composites	1361:1386	protein/polymer composites	1361:1386	Therefore, this study provides a novel method to improve the interfacial compatibility between hydrophobic proteins and hydrophilic polymers, and is expected to expand the application of protein/polymer composites in agriculture and food field.					
36746592	8	32	theme	hydrophilic	1294:1304	arg1	polymers					1306:1313	hydrophilic polymers	1294:1313	hydrophilic polymers	1294:1313	Therefore, this study provides a novel method to improve the interfacial compatibility between hydrophobic proteins and hydrophilic polymers, and is expected to expand the application of protein/polymer composites in agriculture and food field.					
36746592	1	33	contain	have	208:211	arg1	films					173:177	Polysaccharide films	158:177	Polysaccharide films containing protein additives	158:206	Polysaccharide films containing protein additives have good application prospects in agriculture and food field.					
36746592	1	33	contain	have	208:211	arg2	prospects					230:238	good application prospects	213:238	good application prospects	213:238	Polysaccharide films containing protein additives have good application prospects in agriculture and food field.					
36746592	5	34	theme	superior	883:890	arg1	properties					922:931	superior antibacterial and antioxidant properties	883:931	superior antibacterial and antioxidant properties	883:931	And the Cur-ZIF-8/CS/Zein exhibits superior antibacterial and antioxidant properties as well.					
36746592	8	35	theme	interfacial	1235:1245	arg1	compatibility					1247:1259	the interfacial compatibility	1231:1259	the interfacial compatibility between hydrophobic proteins and hydrophilic polymers	1231:1313	Therefore, this study provides a novel method to improve the interfacial compatibility between hydrophobic proteins and hydrophilic polymers, and is expected to expand the application of protein/polymer composites in agriculture and food field.					
36746592	0	36	theme	ZIF-8/chitosan/zein	53:71	arg1	film					73:76	curcumin-loaded ZIF-8/chitosan/zein film	37:76	curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier	37:104	High strength, controlled release of curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier and antibacterial activity for litchi preservation.					
36746592	5	37	theme	antioxidant	910:920	arg1	properties					922:931	superior antibacterial and antioxidant properties	883:931	superior antibacterial and antioxidant properties	883:931	And the Cur-ZIF-8/CS/Zein exhibits superior antibacterial and antioxidant properties as well.					
36746592	3	38	theme	zinc	547:550	arg1	ions					552:555	zinc ions	547:555	zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8)	547:619	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	0	39	theme	curcumin-loaded	37:51	arg1	film					73:76	curcumin-loaded ZIF-8/chitosan/zein film	37:76	curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier	37:104	High strength, controlled release of curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier and antibacterial activity for litchi preservation.					
36746592	8	40	theme	composites	1377:1386	arg1	application					1346:1356	the application	1342:1356	the application of protein/polymer composites in agriculture and food field	1342:1416	Therefore, this study provides a novel method to improve the interfacial compatibility between hydrophobic proteins and hydrophilic polymers, and is expected to expand the application of protein/polymer composites in agriculture and food field.					
36746592	6	41	theme	release	1019:1025	arg1	Cur-ZIF-8/CS/Zein					955:971	Cur-ZIF-8/CS/Zein	955:971	Cur-ZIF-8/CS/Zein	955:971	Importantly, Cur-ZIF-8/CS/Zein can also be used as an intelligent-responsive release platform for curcumin.					
36746592	6	41	theme	release	1019:1025	arg1	platform					1027:1034	an intelligent-responsive release platform	993:1034	an intelligent-responsive release platform for curcumin	993:1047	Importantly, Cur-ZIF-8/CS/Zein can also be used as an intelligent-responsive release platform for curcumin.					
36746592	4	42	theme	synthesized	761:771	arg1	Cur-ZIF-8/CS/Zein					773:789	synthesized Cur-ZIF-8/CS/Zein	761:789	synthesized Cur-ZIF-8/CS/Zein	761:789	With the improvement of interface compatibility, the results show that the elongation at break and O2 barrier property of synthesized Cur-ZIF-8/CS/Zein are 9.2 and 1.5 times higher than CS/Zein, respectively.					
36746592	1	43	theme	food	259:262	arg1	field					264:268	food field	259:268	food field	259:268	Polysaccharide films containing protein additives have good application prospects in agriculture and food field.					
36746592	7	44	theme	litchi	1122:1127	arg1	freshness					1094:1102	freshness	1094:1102	freshness	1094:1102	As a result, Cur-ZIF-8/CS/Zein can keep the freshness and appearance of litchi at least 8 days longer than that of CS/Zein.					
36746592	7	44	theme	litchi	1122:1127	arg1	result					1055:1060	a result	1053:1060	a result	1053:1060	As a result, Cur-ZIF-8/CS/Zein can keep the freshness and appearance of litchi at least 8 days longer than that of CS/Zein.					
36746592	7	44	theme	litchi	1122:1127	arg1	appearance					1108:1117	appearance	1108:1117	appearance	1108:1117	As a result, Cur-ZIF-8/CS/Zein can keep the freshness and appearance of litchi at least 8 days longer than that of CS/Zein.					
36746592	2	45	theme	technical	378:386	arg1	challenge					388:396	a major technical challenge	370:396	a major technical challenge	370:396	However, interfacial incompatibility between hydrophobic proteins and hydrophilic polymers remains a major technical challenge.					
36746592	7	46	dep	8 days	1138:1143	arg1	longer					1145:1150	longer	1145:1150	longer	1145:1150	As a result, Cur-ZIF-8/CS/Zein can keep the freshness and appearance of litchi at least 8 days longer than that of CS/Zein.					
36746592	2	47	theme	interfacial	280:290	arg1	incompatibility					292:306	interfacial incompatibility	280:306	interfacial incompatibility between hydrophobic proteins and hydrophilic polymers	280:360	However, interfacial incompatibility between hydrophobic proteins and hydrophilic polymers remains a major technical challenge.					
36746592	1	48	theme	Polysaccharide	158:171	arg1	films					173:177	Polysaccharide films	158:177	Polysaccharide films containing protein additives	158:206	Polysaccharide films containing protein additives have good application prospects in agriculture and food field.					
36746592	0	49	theme	film	73:76	arg1	release					26:32	release	26:32	release of curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier	26:104	High strength, controlled release of curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier and antibacterial activity for litchi preservation.					
36746592	0	49	theme	film	73:76	arg1	activity					124:131	antibacterial activity	110:131	antibacterial activity for litchi preservation	110:155	High strength, controlled release of curcumin-loaded ZIF-8/chitosan/zein film with excellence gas barrier and antibacterial activity for litchi preservation.					
36746592	6	50	theme	intelligent-responsive	996:1017	arg1	Cur-ZIF-8/CS/Zein					955:971	Cur-ZIF-8/CS/Zein	955:971	Cur-ZIF-8/CS/Zein	955:971	Importantly, Cur-ZIF-8/CS/Zein can also be used as an intelligent-responsive release platform for curcumin.					
36746592	6	50	theme	intelligent-responsive	996:1017	arg1	platform					1027:1034	an intelligent-responsive release platform	993:1034	an intelligent-responsive release platform for curcumin	993:1047	Importantly, Cur-ZIF-8/CS/Zein can also be used as an intelligent-responsive release platform for curcumin.					
36746592	4	51	dep	9.2	795:797	arg1	higher					813:818	higher	813:818	higher	813:818	With the improvement of interface compatibility, the results show that the elongation at break and O2 barrier property of synthesized Cur-ZIF-8/CS/Zein are 9.2 and 1.5 times higher than CS/Zein, respectively.					
36746592	8	52	from	application	1346:1356	arg1	agriculture					1391:1401	agriculture	1391:1401	agriculture	1391:1401	Therefore, this study provides a novel method to improve the interfacial compatibility between hydrophobic proteins and hydrophilic polymers, and is expected to expand the application of protein/polymer composites in agriculture and food field.					
36746592	8	52	from	application	1346:1356	arg1	field					1412:1416	food field	1407:1416	food field	1407:1416	Therefore, this study provides a novel method to improve the interfacial compatibility between hydrophobic proteins and hydrophilic polymers, and is expected to expand the application of protein/polymer composites in agriculture and food field.					
36746592	3	53	theme	imidazolate	585:595	arg1	Cur-ZIF-8					610:618	Cur-ZIF-8	610:618	Cur-ZIF-8	610:618	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	3	53	theme	imidazolate	585:595	arg1	framework-8					597:607	curcumin-loaded zeolitic imidazolate framework-8	560:607	curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8)	560:619	In this work, the interfacial compatibility between hydrophobic zein and hydrophilic chitosan (CS) is improved by the chemical crosslinking between zinc ions of curcumin-loaded zeolitic imidazolate framework-8 (Cur-ZIF-8) with CS and zein.					
36746592	4	54	theme	compatibility	673:685	arg1	improvement					648:658	the improvement	644:658	the improvement of interface compatibility	644:685	With the improvement of interface compatibility, the results show that the elongation at break and O2 barrier property of synthesized Cur-ZIF-8/CS/Zein are 9.2 and 1.5 times higher than CS/Zein, respectively.					
36124906	0	0	theme	oxide-chitosan	140:153	arg1	nanocomposite					155:167	a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite	67:167	a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite	67:167	Electrochemical determination of purine and pyrimidine bases using a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite.					
36124906	4	1	theme	modified	673:680	arg1	electrode					682:690	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode	615:690	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode	615:690	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode showed excellent response toward guanine (G), adenine (A), thymine (T) and cytosine (C).					
36124906	3	2	from	determination	562:574	arg1	DNA					610:612	DNA	610:612	DNA	610:612	Then, the prepared electrode was used as a sensor for simultaneous determination of purine and pyrimidine bases in DNA.					
36124906	0	3	theme	nanoparticles-graphene	117:138	arg1	nanocomposite					155:167	a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite	67:167	a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite	67:167	Electrochemical determination of purine and pyrimidine bases using a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite.					
36124906	4	4	theme	excellent	699:707	arg1	response					709:716	excellent response	699:716	excellent response toward guanine (G), adenine (A), thymine (T) and cytosine (C)	699:778	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode showed excellent response toward guanine (G), adenine (A), thymine (T) and cytosine (C).					
36124906	6	5	theme	detection	940:948	arg1	limits					950:955	The evaluated detection limits	926:955	The evaluated detection limits of G, A, T, and C concentrations	926:988	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	6	5	theme	detection	940:948	arg1	μM					1011:1012	12, 4, 22, and 9 μM	994:1012	12, 4, 22, and 9 μM	994:1012	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	3	6	theme	prepared	505:512	arg1	sensor					538:543	a sensor	536:543	a sensor for simultaneous determination of purine and pyrimidine bases in DNA	536:612	Then, the prepared electrode was used as a sensor for simultaneous determination of purine and pyrimidine bases in DNA.					
36124906	3	6	theme	prepared	505:512	arg1	electrode					514:522	the prepared electrode	501:522	the prepared electrode	501:522	Then, the prepared electrode was used as a sensor for simultaneous determination of purine and pyrimidine bases in DNA.					
36124906	2	7	theme	modified	388:395	arg1	surface					397:403	The modified surface	384:403	The modified surface of the electrode	384:420	The modified surface of the electrode was characterized by field emission-scanning electrochemical microscopy.					
36124906	6	8	theme	T	966:966	arg1	limits					950:955	The evaluated detection limits	926:955	The evaluated detection limits of G, A, T, and C concentrations	926:988	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	6	8	theme	T	966:966	arg1	μM					1011:1012	12, 4, 22, and 9 μM	994:1012	12, 4, 22, and 9 μM	994:1012	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	8	9	theme	pyrimidine	1366:1375	arg1	bases					1377:1381	purine and pyrimidine bases	1355:1381	bases	1377:1381	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	8	10	theme	DNA	1393:1395	arg1	sample					1403:1408	a fish DNA sperm sample	1386:1408	a fish DNA sperm sample	1386:1408	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	8	11	theme	fish	1388:1391	arg1	sample					1403:1408	a fish DNA sperm sample	1386:1408	a fish DNA sperm sample	1386:1408	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	4	12	theme	Phen-Fe<sub>3</sub>O<sub>4</sub>	619:650	arg1	electrode					682:690	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode	615:690	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode	615:690	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode showed excellent response toward guanine (G), adenine (A), thymine (T) and cytosine (C).					
36124906	7	13	theme	modified	1139:1146	arg1	stable					1161:1166	stable	1161:1166	stable	1161:1166	In addition, the results show that the modified electrode is stable for 7 days and has good repeatability and interfering molecules and ions have no significant effect on peak current.					
36124906	7	13	theme	modified	1139:1146	arg1	electrode					1148:1156	the modified electrode	1135:1156	the modified electrode	1135:1156	In addition, the results show that the modified electrode is stable for 7 days and has good repeatability and interfering molecules and ions have no significant effect on peak current.					
36124906	2	14	theme	electrode	412:420	arg1	surface					397:403	The modified surface	384:403	The modified surface of the electrode	384:420	The modified surface of the electrode was characterized by field emission-scanning electrochemical microscopy.					
36124906	6	15	theme	concentrations	975:988	arg1	limits					950:955	The evaluated detection limits	926:955	The evaluated detection limits of G, A, T, and C concentrations	926:988	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	6	15	theme	concentrations	975:988	arg1	μM					1011:1012	12, 4, 22, and 9 μM	994:1012	12, 4, 22, and 9 μM	994:1012	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	8	16	theme	purine	1355:1360	arg1	bases					1377:1381	purine and pyrimidine bases	1355:1381	bases	1377:1381	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	7	17	theme	interfering	1210:1220	arg1	molecules					1222:1230	interfering molecules	1210:1230	interfering molecules	1210:1230	In addition, the results show that the modified electrode is stable for 7 days and has good repeatability and interfering molecules and ions have no significant effect on peak current.					
36124906	4	18	theme	composite	663:671	arg1	electrode					682:690	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode	615:690	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode	615:690	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode showed excellent response toward guanine (G), adenine (A), thymine (T) and cytosine (C).					
36124906	3	19	theme	simultaneous	549:560	arg1	determination					562:574	simultaneous determination	549:574	simultaneous determination of purine and pyrimidine bases in DNA	549:612	Then, the prepared electrode was used as a sensor for simultaneous determination of purine and pyrimidine bases in DNA.					
36124906	7	20	contain	has	1183:1185	arg2	repeatability					1192:1204	good repeatability	1187:1204	good repeatability	1187:1204	In addition, the results show that the modified electrode is stable for 7 days and has good repeatability and interfering molecules and ions have no significant effect on peak current.					
36124906	7	20	contain	has	1183:1185	arg1	stable					1161:1166	stable	1161:1166	stable	1161:1166	In addition, the results show that the modified electrode is stable for 7 days and has good repeatability and interfering molecules and ions have no significant effect on peak current.					
36124906	7	20	contain	has	1183:1185	arg1	electrode					1148:1156	the modified electrode	1135:1156	the modified electrode	1135:1156	In addition, the results show that the modified electrode is stable for 7 days and has good repeatability and interfering molecules and ions have no significant effect on peak current.					
36124906	8	21	theme	bases	1377:1381	arg1	determination					1338:1350	the determination	1334:1350	the determination of purine and pyrimidine bases in a fish DNA sperm sample	1334:1408	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	3	22	theme	pyrimidine	590:599	arg1	bases					601:605	purine and pyrimidine bases	579:605	bases	601:605	Then, the prepared electrode was used as a sensor for simultaneous determination of purine and pyrimidine bases in DNA.					
36124906	7	23	theme	good	1187:1190	arg1	repeatability					1192:1204	good repeatability	1187:1204	good repeatability	1187:1204	In addition, the results show that the modified electrode is stable for 7 days and has good repeatability and interfering molecules and ions have no significant effect on peak current.					
36124906	4	24	theme	NPs-GO-Chi	652:661	arg1	electrode					682:690	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode	615:690	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode	615:690	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode showed excellent response toward guanine (G), adenine (A), thymine (T) and cytosine (C).					
36124906	5	25	theme	larger	874:879	arg1	current					886:892	a larger peak current	872:892	a larger peak current	872:892	The sensor displayed higher effective surface, an appropriate peak-to-peak separation, and a larger peak current compared to the bare electrode.					
36124906	2	26	theme	emission-scanning	449:465	arg1	microscopy					483:492	field emission-scanning electrochemical microscopy	443:492	field emission-scanning electrochemical microscopy	443:492	The modified surface of the electrode was characterized by field emission-scanning electrochemical microscopy.					
36124906	8	27	from	sample	1403:1408	arg1	determination					1338:1350	the determination	1334:1350	the determination of purine and pyrimidine bases in a fish DNA sperm sample	1334:1408	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	0	28	theme	Electrochemical	0:14	arg1	determination					16:28	Electrochemical determination	0:28	Electrochemical determination of purine and pyrimidine bases	0:59	Electrochemical determination of purine and pyrimidine bases using a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite.					
36124906	6	29	theme	concentration	1023:1035	arg1	range					1037:1041	the concentration range	1019:1041	the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM	1019:1083	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	8	30	from	determination	1338:1350	arg1	sample					1403:1408	a fish DNA sperm sample	1386:1408	a fish DNA sperm sample	1386:1408	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	2	31	theme	field	443:447	arg1	microscopy					483:492	field emission-scanning electrochemical microscopy	443:492	field emission-scanning electrochemical microscopy	443:492	The modified surface of the electrode was characterized by field emission-scanning electrochemical microscopy.					
36124906	3	32	theme	purine	579:584	arg1	bases					601:605	purine and pyrimidine bases	579:605	bases	601:605	Then, the prepared electrode was used as a sensor for simultaneous determination of purine and pyrimidine bases in DNA.					
36124906	0	33	theme	purine	33:38	arg1	bases					55:59	purine and pyrimidine bases	33:59	purine and pyrimidine bases	33:59	Electrochemical determination of purine and pyrimidine bases using a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite.					
36124906	3	34	used	used	528:531	arg2	electrode					514:522	the prepared electrode	501:522	the prepared electrode	501:522	Then, the prepared electrode was used as a sensor for simultaneous determination of purine and pyrimidine bases in DNA.					
36124906	3	34	used	used	528:531	arg2	sensor					538:543	a sensor	536:543	a sensor for simultaneous determination of purine and pyrimidine bases in DNA	536:612	Then, the prepared electrode was used as a sensor for simultaneous determination of purine and pyrimidine bases in DNA.					
36124906	6	35	theme	μM	1082:1083	arg1	range					1037:1041	the concentration range	1019:1041	the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM	1019:1083	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	1	36	theme	1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub>	172:218	arg1	nanocomposite					258:270	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite	170:270	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi)	170:316	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi) was fabricated and used to modify a glassy carbon (GC) electrode.					
36124906	1	36	theme	1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub>	172:218	arg1	NPs-GO-Chi					306:315	Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi	273:315	Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi	273:315	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi) was fabricated and used to modify a glassy carbon (GC) electrode.					
36124906	0	37	theme	pyrimidine	44:53	arg1	bases					55:59	purine and pyrimidine bases	33:59	purine and pyrimidine bases	33:59	Electrochemical determination of purine and pyrimidine bases using a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite.					
36124906	5	38	theme	appropriate	831:841	arg1	separation					856:865	an appropriate peak-to-peak separation	828:865	an appropriate peak-to-peak separation	828:865	The sensor displayed higher effective surface, an appropriate peak-to-peak separation, and a larger peak current compared to the bare electrode.					
36124906	3	39	theme	bases	601:605	arg1	determination					562:574	simultaneous determination	549:574	simultaneous determination of purine and pyrimidine bases in DNA	549:612	Then, the prepared electrode was used as a sensor for simultaneous determination of purine and pyrimidine bases in DNA.					
36124906	1	40	theme	glassy	354:359	arg1	electrode					373:381	a glassy carbon (GC) electrode	352:381	a glassy carbon (GC) electrode	352:381	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi) was fabricated and used to modify a glassy carbon (GC) electrode.					
36124906	5	41	theme	peak-to-peak	843:854	arg1	separation					856:865	an appropriate peak-to-peak separation	828:865	an appropriate peak-to-peak separation	828:865	The sensor displayed higher effective surface, an appropriate peak-to-peak separation, and a larger peak current compared to the bare electrode.					
36124906	3	42	from	bases	601:605	arg1	DNA					610:612	DNA	610:612	DNA	610:612	Then, the prepared electrode was used as a sensor for simultaneous determination of purine and pyrimidine bases in DNA.					
36124906	1	43	theme	carbon	361:366	arg1	electrode					373:381	a glassy carbon (GC) electrode	352:381	a glassy carbon (GC) electrode	352:381	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi) was fabricated and used to modify a glassy carbon (GC) electrode.					
36124906	5	44	theme	bare	910:913	arg1	electrode					915:923	the bare electrode	906:923	the bare electrode	906:923	The sensor displayed higher effective surface, an appropriate peak-to-peak separation, and a larger peak current compared to the bare electrode.					
36124906	6	45	theme	A	963:963	arg1	limits					950:955	The evaluated detection limits	926:955	The evaluated detection limits of G, A, T, and C concentrations	926:988	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	6	45	theme	A	963:963	arg1	μM					1011:1012	12, 4, 22, and 9 μM	994:1012	12, 4, 22, and 9 μM	994:1012	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	8	46	theme	real	1415:1418	arg1	sample					1420:1425	a real sample	1413:1425	a real sample	1413:1425	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	8	46	theme	real	1415:1418	arg1	method					1298:1303	The proposed method	1285:1303	The proposed method	1285:1303	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	6	47	theme	C	973:973	arg1	concentrations					975:988	C concentrations	973:988	C concentrations	973:988	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	1	48	theme	nanoparticles-graphene	220:241	arg1	nanocomposite					258:270	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite	170:270	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi)	170:316	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi) was fabricated and used to modify a glassy carbon (GC) electrode.					
36124906	1	48	theme	nanoparticles-graphene	220:241	arg1	NPs-GO-Chi					306:315	Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi	273:315	Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi	273:315	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi) was fabricated and used to modify a glassy carbon (GC) electrode.					
36124906	0	49	theme	bases	55:59	arg1	determination					16:28	Electrochemical determination	0:28	Electrochemical determination of purine and pyrimidine bases	0:59	Electrochemical determination of purine and pyrimidine bases using a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite.					
36124906	5	50	theme	higher	802:807	arg1	surface					819:825	higher effective surface	802:825	higher effective surface	802:825	The sensor displayed higher effective surface, an appropriate peak-to-peak separation, and a larger peak current compared to the bare electrode.					
36124906	3	51	from	DNA	610:612	arg1	determination					562:574	simultaneous determination	549:574	simultaneous determination of purine and pyrimidine bases in DNA	549:612	Then, the prepared electrode was used as a sensor for simultaneous determination of purine and pyrimidine bases in DNA.					
36124906	1	52	theme	oxide-chitosan	243:256	arg1	nanocomposite					258:270	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite	170:270	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi)	170:316	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi) was fabricated and used to modify a glassy carbon (GC) electrode.					
36124906	1	52	theme	oxide-chitosan	243:256	arg1	NPs-GO-Chi					306:315	Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi	273:315	Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi	273:315	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi) was fabricated and used to modify a glassy carbon (GC) electrode.					
36124906	4	53	theme	guanine	725:731	arg1	cytosine					767:774	cytosine	767:774	cytosine (C)	767:778	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode showed excellent response toward guanine (G), adenine (A), thymine (T) and cytosine (C).					
36124906	4	53	theme	guanine	725:731	arg1	thymine					751:757	thymine	751:757	thymine (T)	751:761	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode showed excellent response toward guanine (G), adenine (A), thymine (T) and cytosine (C).					
36124906	4	53	theme	guanine	725:731	arg1	G					734:734	guanine (G)	725:735	guanine (G)	725:735	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode showed excellent response toward guanine (G), adenine (A), thymine (T) and cytosine (C).					
36124906	4	53	theme	guanine	725:731	arg1	adenine					738:744	adenine	738:744	adenine (A)	738:748	The Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi composite modified electrode showed excellent response toward guanine (G), adenine (A), thymine (T) and cytosine (C).					
36124906	5	54	theme	effective	809:817	arg1	surface					819:825	higher effective surface	802:825	higher effective surface	802:825	The sensor displayed higher effective surface, an appropriate peak-to-peak separation, and a larger peak current compared to the bare electrode.					
36124906	6	55	theme	G	960:960	arg1	limits					950:955	The evaluated detection limits	926:955	The evaluated detection limits of G, A, T, and C concentrations	926:988	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	6	55	theme	G	960:960	arg1	μM					1011:1012	12, 4, 22, and 9 μM	994:1012	12, 4, 22, and 9 μM	994:1012	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	6	56	theme	evaluated	930:938	arg1	limits					950:955	The evaluated detection limits	926:955	The evaluated detection limits of G, A, T, and C concentrations	926:988	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	6	56	theme	evaluated	930:938	arg1	μM					1011:1012	12, 4, 22, and 9 μM	994:1012	12, 4, 22, and 9 μM	994:1012	The evaluated detection limits of G, A, T, and C concentrations are 12, 4, 22, and 9 μM over the concentration range of 15-500, 15-400, 40-1000 and 40-1400 μM, respectively.					
36124906	8	57	theme	sperm	1397:1401	arg1	sample					1403:1408	a fish DNA sperm sample	1386:1408	a fish DNA sperm sample	1386:1408	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	8	58	from	bases	1377:1381	arg1	sample					1403:1408	a fish DNA sperm sample	1386:1408	a fish DNA sperm sample	1386:1408	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	1	59	used	used	337:340	arg2	NPs-GO-Chi					306:315	Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi	273:315	Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi	273:315	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi) was fabricated and used to modify a glassy carbon (GC) electrode.					
36124906	1	59	used	used	337:340	arg2	nanocomposite					258:270	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite	170:270	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi)	170:316	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi) was fabricated and used to modify a glassy carbon (GC) electrode.					
36124906	7	60	contain	have	1241:1244	arg1	molecules					1222:1230	interfering molecules	1210:1230	interfering molecules	1210:1230	In addition, the results show that the modified electrode is stable for 7 days and has good repeatability and interfering molecules and ions have no significant effect on peak current.					
36124906	7	60	contain	have	1241:1244	arg2	effect					1261:1266	no significant effect	1246:1266	no significant effect	1246:1266	In addition, the results show that the modified electrode is stable for 7 days and has good repeatability and interfering molecules and ions have no significant effect on peak current.					
36124906	7	60	contain	have	1241:1244	arg1	ions					1236:1239	ions	1236:1239	ions	1236:1239	In addition, the results show that the modified electrode is stable for 7 days and has good repeatability and interfering molecules and ions have no significant effect on peak current.					
36124906	2	61	theme	electrochemical	467:481	arg1	microscopy					483:492	field emission-scanning electrochemical microscopy	443:492	field emission-scanning electrochemical microscopy	443:492	The modified surface of the electrode was characterized by field emission-scanning electrochemical microscopy.					
36124906	1	62	theme	GC	369:370	arg1	electrode					373:381	a glassy carbon (GC) electrode	352:381	a glassy carbon (GC) electrode	352:381	A 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite (Phen-Fe<sub>3</sub>O<sub>4</sub> NPs-GO-Chi) was fabricated and used to modify a glassy carbon (GC) electrode.					
36124906	0	63	theme	1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub>	69:115	arg1	nanocomposite					155:167	a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite	67:167	a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite	67:167	Electrochemical determination of purine and pyrimidine bases using a 1,10-phenanthroline-Fe<sub>3</sub>O<sub>4</sub> nanoparticles-graphene oxide-chitosan nanocomposite.					
36124906	8	64	theme	proposed	1289:1296	arg1	sample					1420:1425	a real sample	1413:1425	a real sample	1413:1425	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	8	64	theme	proposed	1289:1296	arg1	method					1298:1303	The proposed method	1285:1303	The proposed method	1285:1303	The proposed method was successfully applied for the determination of purine and pyrimidine bases in a fish DNA sperm sample as a real sample.					
36124906	7	65	theme	peak	1271:1274	arg1	current					1276:1282	peak current	1271:1282	peak current	1271:1282	In addition, the results show that the modified electrode is stable for 7 days and has good repeatability and interfering molecules and ions have no significant effect on peak current.					
36124906	7	66	theme	significant	1249:1259	arg1	effect					1261:1266	no significant effect	1246:1266	no significant effect	1246:1266	In addition, the results show that the modified electrode is stable for 7 days and has good repeatability and interfering molecules and ions have no significant effect on peak current.					
36124906	5	67	theme	peak	881:884	arg1	current					886:892	a larger peak current	872:892	a larger peak current	872:892	The sensor displayed higher effective surface, an appropriate peak-to-peak separation, and a larger peak current compared to the bare electrode.					
35583764	5	0	theme	CD-DGO	1228:1233	arg1	144 mg·g-1					1276:1285	144 mg·g-1	1276:1285	144 mg·g-1	1276:1285	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	5	0	theme	CD-DGO	1228:1233	arg1	capacity					1216:1223	the maximum adsorption capacity	1193:1223	the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine	1193:1271	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	5	1	theme	adsorption	981:990	arg1	effect					992:997	the adsorption effect	977:997	the adsorption effect of sulfonamides antibiotics	977:1025	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	1	2	theme	composite	287:295	arg1	material					297:304	ternary composite material	279:304	ternary composite material	279:304	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	0	3	theme	sulfonamide	102:112	arg1	antibiotics					114:124	sulfonamide antibiotics	102:124	sulfonamide antibiotics	102:124	Preparation of β-cyclodextrin/dopamine hydrochloride-graphene oxide and its adsorption properties for sulfonamide antibiotics.					
35583764	6	4	theme	dominant	1360:1367	arg1	force					1369:1373	the dominant force	1356:1373	the dominant force in the adsorption process	1356:1399	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	6	4	theme	dominant	1360:1367	arg1	effect					1433:1438	the π-π electron conjugation effect	1404:1438	the π-π electron conjugation effect with hydrogen bonding	1404:1460	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	2	5	theme	electron	416:423	arg1	microscopy					425:434	scanning electron microscopy	407:434	scanning electron microscopy	407:434	The material was characterized using scanning electron microscopy, Fourier infrared spectrometry, transmission electron microscopy, and specific surface area optical analysis.					
35583764	1	6	theme	material	297:304	arg1	type					271:274	a new type	265:274	a new type of ternary composite material	265:304	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	5	7	theme	kinetic	1105:1111	arg1	model					1113:1117	the pseudo-second-order kinetic model	1081:1117	the pseudo-second-order kinetic model	1081:1117	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	6	8	with	effect	1433:1438	arg1	bonding					1454:1460	hydrogen bonding	1445:1460	hydrogen bonding	1445:1460	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	6	9	theme	adsorption	1382:1391	arg1	process					1393:1399	the adsorption process	1378:1399	the adsorption process	1378:1399	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	4	10	theme	adsorption	826:835	arg1	isotherms					837:845	adsorption isotherms	826:845	adsorption isotherms	826:845	We analyzed the adsorption characteristics via adsorption kinetics and adsorption isotherms, and then investigated the stability of the adsorbent through desorption and regeneration of the adsorbent.					
35583764	5	11	theme	adsorption	1050:1059	arg1	kinetics					1061:1068	the adsorption kinetics	1046:1068	the adsorption kinetics	1046:1068	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	3	12	dep	antibiotics	571:581	arg1	sulfadiazine					602:613	sulfadiazine	602:613	sulfadiazine	602:613	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	3	12	dep	antibiotics	571:581	arg1	sulfamethoxazole					584:599	sulfamethoxazole	584:599	sulfamethoxazole	584:599	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	1	13	theme	β-cyclodextrin/dopamine	307:329	arg1	CD-DGO					361:366	CD-DGO	361:366	CD-DGO	361:366	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	1	13	theme	β-cyclodextrin/dopamine	307:329	arg1	oxide					354:358	β-cyclodextrin/dopamine hydrochloride-graphene oxide	307:358	β-cyclodextrin/dopamine hydrochloride-graphene oxide	307:358	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	1	14	theme	antibiotic	154:163	arg1	adsorbents					165:174	high-efficiency antibiotic adsorbents	138:174	high-efficiency antibiotic adsorbents	138:174	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	7	15	theme	water	1541:1545	arg1	pollution					1547:1555	antibiotics water pollution	1529:1555	sulfonamides antibiotics water pollution	1516:1555	This offers a theoretical basis for the treatment of sulfonamides antibiotics water pollution.					
35583764	5	16	theme	antibiotics	1015:1025	arg1	effect					992:997	the adsorption effect	977:997	the adsorption effect of sulfonamides antibiotics	977:1025	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	2	17	theme	transmission	468:479	arg1	microscopy					490:499	transmission electron microscopy	468:499	transmission electron microscopy	468:499	The material was characterized using scanning electron microscopy, Fourier infrared spectrometry, transmission electron microscopy, and specific surface area optical analysis.					
35583764	6	18	from	force	1369:1373	arg1	process					1393:1399	the adsorption process	1378:1399	the adsorption process	1378:1399	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	5	19	theme	adsorption	1205:1214	arg1	144 mg·g-1					1276:1285	144 mg·g-1	1276:1285	144 mg·g-1	1276:1285	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	5	19	theme	adsorption	1205:1214	arg1	capacity					1216:1223	the maximum adsorption capacity	1193:1223	the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine	1193:1271	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	2	20	theme	optical	528:534	arg1	analysis					536:543	specific surface area optical analysis	506:543	specific surface area optical analysis	506:543	The material was characterized using scanning electron microscopy, Fourier infrared spectrometry, transmission electron microscopy, and specific surface area optical analysis.					
35583764	3	21	theme	adsorption	616:625	arg1	capacity					627:634	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity	546:634	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity	546:634	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	4	22	theme	adsorbent	944:952	arg1	regeneration					924:935	regeneration	924:935	regeneration	924:935	We analyzed the adsorption characteristics via adsorption kinetics and adsorption isotherms, and then investigated the stability of the adsorbent through desorption and regeneration of the adsorbent.					
35583764	4	22	theme	adsorbent	944:952	arg1	desorption					909:918	desorption	909:918	desorption	909:918	We analyzed the adsorption characteristics via adsorption kinetics and adsorption isotherms, and then investigated the stability of the adsorbent through desorption and regeneration of the adsorbent.					
35583764	7	23	theme	sulfonamides	1516:1527	arg1	treatment					1503:1511	the treatment	1499:1511	the treatment of sulfonamides antibiotics water pollution	1499:1555	This offers a theoretical basis for the treatment of sulfonamides antibiotics water pollution.					
35583764	5	24	theme	maximum	1197:1203	arg1	144 mg·g-1					1276:1285	144 mg·g-1	1276:1285	144 mg·g-1	1276:1285	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	5	24	theme	maximum	1197:1203	arg1	capacity					1216:1223	the maximum adsorption capacity	1193:1223	the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine	1193:1271	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	2	25	theme	area	523:526	arg1	analysis					536:543	specific surface area optical analysis	506:543	specific surface area optical analysis	506:543	The material was characterized using scanning electron microscopy, Fourier infrared spectrometry, transmission electron microscopy, and specific surface area optical analysis.					
35583764	1	26	theme	hydrochloride-graphene	331:352	arg1	CD-DGO					361:366	CD-DGO	361:366	CD-DGO	361:366	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	1	26	theme	hydrochloride-graphene	331:352	arg1	oxide					354:358	β-cyclodextrin/dopamine hydrochloride-graphene oxide	307:358	β-cyclodextrin/dopamine hydrochloride-graphene oxide	307:358	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	3	27	theme	dosage	667:672	arg1	terms					654:658	terms	654:658	terms of the dosage of composite materials, the ratio of each component, and the pH of the solution	654:752	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	2	28	theme	infrared	445:452	arg1	spectrometry					454:465	Fourier infrared spectrometry	437:465	Fourier infrared spectrometry	437:465	The material was characterized using scanning electron microscopy, Fourier infrared spectrometry, transmission electron microscopy, and specific surface area optical analysis.					
35583764	0	29	theme	hydrochloride-graphene	39:60	arg1	oxide					62:66	β-cyclodextrin/dopamine hydrochloride-graphene oxide	15:66	β-cyclodextrin/dopamine hydrochloride-graphene oxide	15:66	Preparation of β-cyclodextrin/dopamine hydrochloride-graphene oxide and its adsorption properties for sulfonamide antibiotics.					
35583764	7	30	theme	antibiotics	1529:1539	arg1	pollution					1547:1555	antibiotics water pollution	1529:1555	sulfonamides antibiotics water pollution	1516:1555	This offers a theoretical basis for the treatment of sulfonamides antibiotics water pollution.					
35583764	6	31	theme	good	1334:1337	arg1	reusability					1339:1349	good reusability	1334:1349	good reusability	1334:1349	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	0	32	theme	β-cyclodextrin/dopamine	15:37	arg1	oxide					62:66	β-cyclodextrin/dopamine hydrochloride-graphene oxide	15:66	β-cyclodextrin/dopamine hydrochloride-graphene oxide	15:66	Preparation of β-cyclodextrin/dopamine hydrochloride-graphene oxide and its adsorption properties for sulfonamide antibiotics.					
35583764	6	33	theme	hydrogen	1445:1452	arg1	bonding					1454:1460	hydrogen bonding	1445:1460	hydrogen bonding	1445:1460	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	5	34	theme	Langmuir	1163:1170	arg1	adsorption					1172:1181	the Langmuir adsorption	1159:1181	the Langmuir adsorption isotherm	1159:1190	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	1	35	theme	high-efficiency	138:152	arg1	adsorbents					165:174	high-efficiency antibiotic adsorbents	138:174	high-efficiency antibiotic adsorbents	138:174	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	3	36	theme	typical	550:556	arg1	antibiotics					571:581	Two typical sulfonamides antibiotics	546:581	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity	546:634	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	5	37	theme	sulfonamides	1002:1013	arg1	antibiotics					1015:1025	sulfonamides antibiotics	1002:1025	sulfonamides antibiotics	1002:1025	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	5	38	theme	adsorption	1172:1181	arg1	isotherm					1183:1190	the Langmuir adsorption isotherm	1159:1190	the Langmuir adsorption isotherm	1159:1190	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	3	39	theme	materials	687:695	arg1	ratio					702:706	the ratio	698:706	the ratio of each component	698:724	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	3	39	theme	materials	687:695	arg1	pH					735:736	the pH	731:736	the pH of the solution	731:752	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	3	39	theme	materials	687:695	arg1	dosage					667:672	the dosage	663:672	the dosage of composite materials	663:695	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	1	40	theme	graphene	239:246	arg1	oxide					248:252	graphene oxide	239:252	graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO)	239:367	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	0	41	theme	oxide	62:66	arg1	Preparation					0:10	Preparation	0:10	Preparation of β-cyclodextrin/dopamine hydrochloride-graphene oxide and its adsorption properties for sulfonamide antibiotics.	0:125	Preparation of β-cyclodextrin/dopamine hydrochloride-graphene oxide and its adsorption properties for sulfonamide antibiotics.					
35583764	3	42	theme	component	716:724	arg1	ratio					702:706	the ratio	698:706	the ratio of each component	698:724	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	3	42	theme	component	716:724	arg1	pH					735:736	the pH	731:736	the pH of the solution	731:752	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	3	42	theme	component	716:724	arg1	dosage					667:672	the dosage	663:672	the dosage of composite materials	663:695	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	2	43	theme	surface	515:521	arg1	analysis					536:543	specific surface area optical analysis	506:543	specific surface area optical analysis	506:543	The material was characterized using scanning electron microscopy, Fourier infrared spectrometry, transmission electron microscopy, and specific surface area optical analysis.					
35583764	2	44	theme	electron	481:488	arg1	microscopy					490:499	transmission electron microscopy	468:499	transmission electron microscopy	468:499	The material was characterized using scanning electron microscopy, Fourier infrared spectrometry, transmission electron microscopy, and specific surface area optical analysis.					
35583764	0	45	theme	adsorption	76:85	arg1	properties					87:96	its adsorption properties	72:96	its adsorption properties	72:96	Preparation of β-cyclodextrin/dopamine hydrochloride-graphene oxide and its adsorption properties for sulfonamide antibiotics.					
35583764	6	46	contain	has	1330:1332	arg1	material					1321:1328	The material	1317:1328	The material	1317:1328	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	6	46	contain	has	1330:1332	arg2	reusability					1339:1349	good reusability	1334:1349	good reusability	1334:1349	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	6	47	theme	conjugation	1421:1431	arg1	force					1369:1373	the dominant force	1356:1373	the dominant force in the adsorption process	1356:1399	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	6	47	theme	conjugation	1421:1431	arg1	effect					1433:1438	the π-π electron conjugation effect	1404:1438	the π-π electron conjugation effect with hydrogen bonding	1404:1460	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	7	48	dep	sulfonamides	1516:1527	arg1	pollution					1547:1555	antibiotics water pollution	1529:1555	sulfonamides antibiotics water pollution	1516:1555	This offers a theoretical basis for the treatment of sulfonamides antibiotics water pollution.					
35583764	7	49	theme	theoretical	1477:1487	arg1	basis					1489:1493	a theoretical basis	1475:1493	a theoretical basis for the treatment of sulfonamides antibiotics water pollution	1475:1555	This offers a theoretical basis for the treatment of sulfonamides antibiotics water pollution.					
35583764	2	50	theme	scanning	407:414	arg1	microscopy					425:434	scanning electron microscopy	407:434	scanning electron microscopy	407:434	The material was characterized using scanning electron microscopy, Fourier infrared spectrometry, transmission electron microscopy, and specific surface area optical analysis.					
35583764	6	51	theme	electron	1412:1419	arg1	force					1369:1373	the dominant force	1356:1373	the dominant force in the adsorption process	1356:1399	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	6	51	theme	electron	1412:1419	arg1	effect					1433:1438	the π-π electron conjugation effect	1404:1438	the π-π electron conjugation effect with hydrogen bonding	1404:1460	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	5	52	theme	adsorption	1128:1137	arg1	equilibrium					1139:1149	the adsorption equilibrium	1124:1149	the adsorption equilibrium	1124:1149	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	3	53	theme	composite	677:685	arg1	materials					687:695	composite materials	677:695	composite materials	677:695	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	4	54	theme	adsorption	771:780	arg1	characteristics					782:796	the adsorption characteristics	767:796	the adsorption characteristics	767:796	We analyzed the adsorption characteristics via adsorption kinetics and adsorption isotherms, and then investigated the stability of the adsorbent through desorption and regeneration of the adsorbent.					
35583764	3	55	theme	ratio	702:706	arg1	terms					654:658	terms	654:658	terms of the dosage of composite materials, the ratio of each component, and the pH of the solution	654:752	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	6	56	theme	π-π	1408:1410	arg1	force					1369:1373	the dominant force	1356:1373	the dominant force in the adsorption process	1356:1399	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	6	56	theme	π-π	1408:1410	arg1	effect					1433:1438	the π-π electron conjugation effect	1404:1438	the π-π electron conjugation effect with hydrogen bonding	1404:1460	The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding.					
35583764	3	57	theme	solution	745:752	arg1	ratio					702:706	the ratio	698:706	the ratio of each component	698:724	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	3	57	theme	solution	745:752	arg1	pH					735:736	the pH	731:736	the pH of the solution	731:752	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	3	57	theme	solution	745:752	arg1	dosage					667:672	the dosage	663:672	the dosage of composite materials	663:695	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	1	58	dep	prepare	257:263	arg1	CD-DGO					361:366	CD-DGO	361:366	CD-DGO	361:366	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	1	58	dep	prepare	257:263	arg1	oxide					354:358	β-cyclodextrin/dopamine hydrochloride-graphene oxide	307:358	β-cyclodextrin/dopamine hydrochloride-graphene oxide	307:358	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	4	59	theme	adsorbent	891:899	arg1	stability					874:882	the stability	870:882	the stability of the adsorbent	870:899	We analyzed the adsorption characteristics via adsorption kinetics and adsorption isotherms, and then investigated the stability of the adsorbent through desorption and regeneration of the adsorbent.					
35583764	3	60	theme	sulfonamides	558:569	arg1	antibiotics					571:581	Two typical sulfonamides antibiotics	546:581	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity	546:634	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	1	61	theme	new	267:269	arg1	type					271:274	a new type	265:274	a new type of ternary composite material	265:304	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	3	62	theme	pH	735:736	arg1	terms					654:658	terms	654:658	terms of the dosage of composite materials, the ratio of each component, and the pH of the solution	654:752	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	3	63	theme	antibiotics	571:581	arg1	capacity					627:634	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity	546:634	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity	546:634	Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution.					
35583764	4	64	theme	adsorption	802:811	arg1	kinetics					813:820	adsorption kinetics	802:820	adsorption kinetics	802:820	We analyzed the adsorption characteristics via adsorption kinetics and adsorption isotherms, and then investigated the stability of the adsorbent through desorption and regeneration of the adsorbent.					
35583764	5	65	theme	pseudo-second-order	1085:1103	arg1	model					1113:1117	the pseudo-second-order kinetic model	1081:1117	the pseudo-second-order kinetic model	1081:1117	The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g-1 and 152 mg·g-1, respectively.					
35583764	2	66	theme	specific	506:513	arg1	analysis					536:543	specific surface area optical analysis	506:543	specific surface area optical analysis	506:543	The material was characterized using scanning electron microscopy, Fourier infrared spectrometry, transmission electron microscopy, and specific surface area optical analysis.					
35583764	2	67	theme	Fourier	437:443	arg1	spectrometry					454:465	Fourier infrared spectrometry	437:465	Fourier infrared spectrometry	437:465	The material was characterized using scanning electron microscopy, Fourier infrared spectrometry, transmission electron microscopy, and specific surface area optical analysis.					
35583764	0	68	theme	properties	87:96	arg1	Preparation					0:10	Preparation	0:10	Preparation of β-cyclodextrin/dopamine hydrochloride-graphene oxide and its adsorption properties for sulfonamide antibiotics.	0:125	Preparation of β-cyclodextrin/dopamine hydrochloride-graphene oxide and its adsorption properties for sulfonamide antibiotics.					
35583764	1	69	used	used	224:227	arg2	hydrochloride					205:217	dopamine hydrochloride	196:217	dopamine hydrochloride	196:217	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	1	69	used	used	224:227	arg2	β-cyclodextrin					177:190	β-cyclodextrin	177:190	β-cyclodextrin	177:190	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	1	70	theme	dopamine	196:203	arg1	hydrochloride					205:217	dopamine hydrochloride	196:217	dopamine hydrochloride	196:217	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35583764	1	71	theme	ternary	279:285	arg1	material					297:304	ternary composite material	279:304	ternary composite material	279:304	To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO).					
35333441	4	0	theme	Fat	567:569	arg1	FE					580:581	FE	580:581	FE	580:581	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	4	0	theme	Fat	567:569	arg1	extract					571:577	Fat extract	567:577	Fat extract (FE)	567:582	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	4	0	theme	Fat	567:569	arg1	component					606:614	a cell-free liquid component	587:614	a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods	587:705	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	7	1	theme	M2	1193:1194	arg1	phenotype					1196:1204	an anti-inflammatory M2 phenotype	1172:1204	an anti-inflammatory M2 phenotype	1172:1204	Specifically, FE promotes the polarization of macrophages from an inflammatory M1 phenotype to an anti-inflammatory M2 phenotype.					
35333441	6	2	theme	macrophages	1034:1044	arg1	phenotype					1021:1029	the inflammatory phenotype	1004:1029	the inflammatory phenotype of macrophages in the locally injured region	1004:1074	The composite not only inhibits death of neuro- and vascular cells and leads to the preservation of neural and vascular structure, but also modulates the inflammatory phenotype of macrophages in the locally injured region.					
35333441	7	3	theme	M1	1156:1157	arg1	phenotype					1159:1167	an inflammatory M1 phenotype	1140:1167	an inflammatory M1 phenotype to an anti-inflammatory M2 phenotype	1140:1204	Specifically, FE promotes the polarization of macrophages from an inflammatory M1 phenotype to an anti-inflammatory M2 phenotype.					
35333441	1	4	theme	secondary	324:332	arg1	inflammation					334:345	secondary inflammation	324:345	secondary inflammation	324:345	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	9	5	theme	promising	1389:1397	arg1	treatment					1399:1407	a promising treatment	1387:1407	a promising treatment for SCI	1387:1415	In summary, FE is a promising treatment for SCI, as it is easy to obtain, nonimmunogenic, and effective.					
35333441	9	5	theme	promising	1389:1397	arg1	FE					1381:1382	FE	1381:1382	FE	1381:1382	In summary, FE is a promising treatment for SCI, as it is easy to obtain, nonimmunogenic, and effective.					
35333441	8	6	theme	STAT6/Arg-1	1296:1306	arg1	pathway					1318:1324	the STAT6/Arg-1 signaling pathway	1292:1324	the STAT6/Arg-1 signaling pathway	1292:1324	During the screening of the involved pathways, it is corroborated that activation of the STAT6/Arg-1 signaling pathway is involved in macrophage M2 polarization.					
35333441	7	7	theme	inflammatory	1143:1154	arg1	phenotype					1159:1167	an inflammatory M1 phenotype	1140:1167	an inflammatory M1 phenotype to an anti-inflammatory M2 phenotype	1140:1204	Specifically, FE promotes the polarization of macrophages from an inflammatory M1 phenotype to an anti-inflammatory M2 phenotype.					
35333441	3	8	theme	excessive	533:541	arg1	responses					556:564	excessive inflammatory responses	533:564	excessive inflammatory responses	533:564	Bioactive materials support the injured spinal cord, stabilize the morphology, and avoid excessive inflammatory responses.					
35333441	6	9	theme	injured	1061:1067	arg1	region					1069:1074	the locally injured region	1049:1074	the locally injured region	1049:1074	The composite not only inhibits death of neuro- and vascular cells and leads to the preservation of neural and vascular structure, but also modulates the inflammatory phenotype of macrophages in the locally injured region.					
35333441	0	10	theme	Cord	109:112	arg1	Model					121:125	a Spinal Cord Injury Model	100:125	a Spinal Cord Injury Model	100:125	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.					
35333441	5	11	from	mice	848:851	arg1	model					814:818	a model	812:818	a model of spinal cord contusion in mice	812:851	In this research, a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE is used to treat a model of spinal cord contusion in mice.					
35333441	8	12	theme	pathway	1318:1324	arg1	activation					1278:1287	activation	1278:1287	activation of the STAT6/Arg-1 signaling pathway	1278:1324	During the screening of the involved pathways, it is corroborated that activation of the STAT6/Arg-1 signaling pathway is involved in macrophage M2 polarization.					
35333441	5	13	theme	cord	830:833	arg1	contusion					835:843	spinal cord contusion	823:843	spinal cord contusion in mice	823:851	In this research, a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE is used to treat a model of spinal cord contusion in mice.					
35333441	4	14	theme	cytokines	640:648	arg1	cytokines					640:648	cytokines	640:648	cytokines extracted from human fat tissue using mechanical methods	640:705	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	4	14	theme	cytokines	640:648	arg1	variety					629:635	a variety	627:635	a variety of cytokines extracted from human fat tissue using mechanical methods	627:705	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	0	15	theme	Spinal	102:107	arg1	Cord					109:112	a Spinal Cord	100:112	a Spinal Cord Injury Model	100:125	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.					
35333441	0	16	from	Tissue	34:39	arg1	Extracts					10:17	Cell-Free Extracts	0:17	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel	0:72	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.					
35333441	8	17	theme	signaling	1308:1316	arg1	pathway					1318:1324	the STAT6/Arg-1 signaling pathway	1292:1324	the STAT6/Arg-1 signaling pathway	1292:1324	During the screening of the involved pathways, it is corroborated that activation of the STAT6/Arg-1 signaling pathway is involved in macrophage M2 polarization.					
35333441	1	18	theme	spinal	187:192	arg1	SCIs					209:212	SCIs	209:212	SCIs	209:212	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	1	18	theme	spinal	187:192	arg1	injuries					199:206	spinal cord injuries	187:206	spinal cord injuries (SCIs)	187:213	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	2	19	theme	cytokines	433:441	arg1	combination					394:404	the combination	390:404	the combination of bioactive materials and cytokines	390:441	The current promising strategy for SCI is the combination of bioactive materials and cytokines.					
35333441	2	19	theme	cytokines	433:441	arg1	strategy					370:377	The current promising strategy	348:377	The current promising strategy for SCI	348:385	The current promising strategy for SCI is the combination of bioactive materials and cytokines.					
35333441	7	20	theme	anti-inflammatory	1175:1191	arg1	phenotype					1196:1204	an anti-inflammatory M2 phenotype	1172:1204	an anti-inflammatory M2 phenotype	1172:1204	Specifically, FE promotes the polarization of macrophages from an inflammatory M1 phenotype to an anti-inflammatory M2 phenotype.					
35333441	6	21	theme	inflammatory	1008:1019	arg1	phenotype					1021:1029	the inflammatory phenotype	1004:1029	the inflammatory phenotype of macrophages in the locally injured region	1004:1074	The composite not only inhibits death of neuro- and vascular cells and leads to the preservation of neural and vascular structure, but also modulates the inflammatory phenotype of macrophages in the locally injured region.					
35333441	1	22	theme	cord	194:197	arg1	SCIs					209:212	SCIs	209:212	SCIs	209:212	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	1	22	theme	cord	194:197	arg1	injuries					199:206	spinal cord injuries	187:206	spinal cord injuries (SCIs)	187:213	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	0	23	theme	Cell-Free	0:8	arg1	Extracts					10:17	Cell-Free Extracts	0:17	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel	0:72	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.					
35333441	2	24	theme	promising	360:368	arg1	combination					394:404	the combination	390:404	the combination of bioactive materials and cytokines	390:441	The current promising strategy for SCI is the combination of bioactive materials and cytokines.					
35333441	2	24	theme	promising	360:368	arg1	strategy					370:377	The current promising strategy	348:377	The current promising strategy for SCI	348:385	The current promising strategy for SCI is the combination of bioactive materials and cytokines.					
35333441	0	25	theme	Human	24:28	arg1	Tissue					34:39	Human Fat Tissue	24:39	Human Fat Tissue with a Hyaluronan-Based Hydrogel	24:72	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.					
35333441	1	26	theme	nerve	269:273	arg1	tissue					275:280	nerve tissue	269:280	nerve tissue	269:280	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	6	27	theme	cells	915:919	arg1	death					886:890	death	886:890	death of neuro- and vascular cells	886:919	The composite not only inhibits death of neuro- and vascular cells and leads to the preservation of neural and vascular structure, but also modulates the inflammatory phenotype of macrophages in the locally injured region.					
35333441	6	28	from	phenotype	1021:1029	arg1	region					1069:1074	the locally injured region	1049:1074	the locally injured region	1049:1074	The composite not only inhibits death of neuro- and vascular cells and leads to the preservation of neural and vascular structure, but also modulates the inflammatory phenotype of macrophages in the locally injured region.					
35333441	3	29	theme	inflammatory	543:554	arg1	responses					556:564	excessive inflammatory responses	533:564	excessive inflammatory responses	533:564	Bioactive materials support the injured spinal cord, stabilize the morphology, and avoid excessive inflammatory responses.					
35333441	2	30	theme	bioactive	409:417	arg1	materials					419:427	bioactive materials	409:427	bioactive materials	409:427	The current promising strategy for SCI is the combination of bioactive materials and cytokines.					
35333441	1	31	theme	tissue	275:280	arg1	formation					294:302	glial scar formation	283:302	glial scar formation	283:302	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	1	31	theme	tissue	275:280	arg1	ischemia					311:318	local ischemia	305:318	local ischemia	305:318	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	1	31	theme	tissue	275:280	arg1	inflammation					334:345	secondary inflammation	324:345	secondary inflammation	324:345	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	1	31	theme	tissue	275:280	arg1	loss					261:264	a loss	259:264	a loss of nerve tissue	259:280	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	0	32	theme	Injury	114:119	arg1	Model					121:125	a Spinal Cord Injury Model	100:125	a Spinal Cord Injury Model	100:125	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.					
35333441	4	33	theme	fat	671:673	arg1	tissue					675:680	human fat tissue	665:680	human fat tissue using mechanical methods	665:705	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	6	34	theme	vascular	906:913	arg1	cells					915:919	neuro- and vascular cells	895:919	neuro- and vascular cells	895:919	The composite not only inhibits death of neuro- and vascular cells and leads to the preservation of neural and vascular structure, but also modulates the inflammatory phenotype of macrophages in the locally injured region.					
35333441	0	35	theme	M2	135:136	arg1	Polarization					159:170	M2 Microglia/Microphage Polarization	135:170	M2 Microglia/Microphage Polarization	135:170	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.					
35333441	5	36	theme	contusion	835:843	arg1	model					814:818	a model	812:818	a model of spinal cord contusion in mice	812:851	In this research, a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE is used to treat a model of spinal cord contusion in mice.					
35333441	8	37	theme	involved	1235:1242	arg1	pathways					1244:1251	the involved pathways	1231:1251	the involved pathways	1231:1251	During the screening of the involved pathways, it is corroborated that activation of the STAT6/Arg-1 signaling pathway is involved in macrophage M2 polarization.					
35333441	0	38	theme	Fat	30:32	arg1	Tissue					34:39	Human Fat Tissue	24:39	Human Fat Tissue with a Hyaluronan-Based Hydrogel	24:72	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.					
35333441	1	39	theme	glial	283:287	arg1	formation					294:302	glial scar formation	283:302	glial scar formation	283:302	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	5	40	dep	HAMC	742:745	arg1	methylcellulose					763:777	methylcellulose	763:777	methylcellulose	763:777	In this research, a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE is used to treat a model of spinal cord contusion in mice.					
35333441	5	40	dep	HAMC	742:745	arg1	hyaluronan					748:757	hyaluronan	748:757	hyaluronan	748:757	In this research, a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE is used to treat a model of spinal cord contusion in mice.					
35333441	2	41	theme	materials	419:427	arg1	combination					394:404	the combination	390:404	the combination of bioactive materials and cytokines	390:441	The current promising strategy for SCI is the combination of bioactive materials and cytokines.					
35333441	2	41	theme	materials	419:427	arg1	strategy					370:377	The current promising strategy	348:377	The current promising strategy for SCI	348:385	The current promising strategy for SCI is the combination of bioactive materials and cytokines.					
35333441	3	42	theme	Bioactive	444:452	arg1	materials					454:462	Bioactive materials	444:462	Bioactive materials	444:462	Bioactive materials support the injured spinal cord, stabilize the morphology, and avoid excessive inflammatory responses.					
35333441	1	43	theme	scar	289:292	arg1	formation					294:302	glial scar formation	283:302	glial scar formation	283:302	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	5	44	used	used	798:801	arg2	HAMC					742:745	a biocompatible HAMC	726:745	a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE	726:793	In this research, a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE is used to treat a model of spinal cord contusion in mice.					
35333441	4	45	contain	containing	616:625	arg1	extract					571:577	Fat extract	567:577	Fat extract (FE)	567:582	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	4	45	contain	containing	616:625	arg1	component					606:614	a cell-free liquid component	587:614	a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods	587:705	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	4	45	contain	containing	616:625	arg2	variety					629:635	a variety	627:635	a variety of cytokines extracted from human fat tissue using mechanical methods	627:705	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	4	45	contain	containing	616:625	arg2	cytokines					640:648	cytokines	640:648	cytokines extracted from human fat tissue using mechanical methods	640:705	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	5	46	from	model	814:818	arg1	mice					848:851	mice	848:851	mice	848:851	In this research, a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE is used to treat a model of spinal cord contusion in mice.					
35333441	3	47	theme	spinal	484:489	arg1	cord					491:494	the injured spinal cord	472:494	the injured spinal cord	472:494	Bioactive materials support the injured spinal cord, stabilize the morphology, and avoid excessive inflammatory responses.					
35333441	0	48	theme	Microglia/Microphage	138:157	arg1	Polarization					159:170	M2 Microglia/Microphage Polarization	135:170	M2 Microglia/Microphage Polarization	135:170	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.					
35333441	4	49	theme	mechanical	688:697	arg1	methods					699:705	mechanical methods	688:705	mechanical methods	688:705	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	8	50	theme	M2	1352:1353	arg1	polarization					1355:1366	macrophage M2 polarization	1341:1366	macrophage M2 polarization	1341:1366	During the screening of the involved pathways, it is corroborated that activation of the STAT6/Arg-1 signaling pathway is involved in macrophage M2 polarization.					
35333441	8	51	theme	macrophage	1341:1350	arg1	polarization					1355:1366	macrophage M2 polarization	1341:1366	macrophage M2 polarization	1341:1366	During the screening of the involved pathways, it is corroborated that activation of the STAT6/Arg-1 signaling pathway is involved in macrophage M2 polarization.					
35333441	6	52	theme	structure	974:982	arg1	preservation					938:949	the preservation	934:949	the preservation of neural and vascular structure	934:982	The composite not only inhibits death of neuro- and vascular cells and leads to the preservation of neural and vascular structure, but also modulates the inflammatory phenotype of macrophages in the locally injured region.					
35333441	7	53	theme	macrophages	1123:1133	arg1	polarization					1107:1118	the polarization	1103:1118	the polarization of macrophages	1103:1133	Specifically, FE promotes the polarization of macrophages from an inflammatory M1 phenotype to an anti-inflammatory M2 phenotype.					
35333441	9	54	from	treatment	1399:1407	arg1	summary					1372:1378	summary	1372:1378	summary	1372:1378	In summary, FE is a promising treatment for SCI, as it is easy to obtain, nonimmunogenic, and effective.					
35333441	3	55	theme	injured	476:482	arg1	cord					491:494	the injured spinal cord	472:494	the injured spinal cord	472:494	Bioactive materials support the injured spinal cord, stabilize the morphology, and avoid excessive inflammatory responses.					
35333441	6	56	theme	vascular	965:972	arg1	structure					974:982	neural and vascular structure	954:982	neural and vascular structure	954:982	The composite not only inhibits death of neuro- and vascular cells and leads to the preservation of neural and vascular structure, but also modulates the inflammatory phenotype of macrophages in the locally injured region.					
35333441	5	57	theme	biocompatible	728:740	arg1	HAMC					742:745	a biocompatible HAMC	726:745	a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE	726:793	In this research, a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE is used to treat a model of spinal cord contusion in mice.					
35333441	8	58	theme	pathways	1244:1251	arg1	screening					1218:1226	the screening	1214:1226	the screening of the involved pathways	1214:1251	During the screening of the involved pathways, it is corroborated that activation of the STAT6/Arg-1 signaling pathway is involved in macrophage M2 polarization.					
35333441	5	59	from	contusion	835:843	arg1	mice					848:851	mice	848:851	mice	848:851	In this research, a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE is used to treat a model of spinal cord contusion in mice.					
35333441	2	60	theme	current	352:358	arg1	combination					394:404	the combination	390:404	the combination of bioactive materials and cytokines	390:441	The current promising strategy for SCI is the combination of bioactive materials and cytokines.					
35333441	2	60	theme	current	352:358	arg1	strategy					370:377	The current promising strategy	348:377	The current promising strategy for SCI	348:385	The current promising strategy for SCI is the combination of bioactive materials and cytokines.					
35333441	5	61	theme	spinal	823:828	arg1	cord					830:833	spinal cord	823:833	spinal cord contusion in mice	823:851	In this research, a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE is used to treat a model of spinal cord contusion in mice.					
35333441	4	62	theme	liquid	599:604	arg1	component					606:614	a cell-free liquid component	587:614	a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods	587:705	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	4	62	theme	liquid	599:604	arg1	extract					571:577	Fat extract	567:577	Fat extract (FE)	567:582	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	0	63	theme	Hyaluronan-Based	48:63	arg1	Hydrogel					65:72	a Hyaluronan-Based Hydrogel	46:72	a Hyaluronan-Based Hydrogel	46:72	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.					
35333441	1	64	theme	local	305:309	arg1	ischemia					311:318	local ischemia	305:318	local ischemia	305:318	Treatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation.					
35333441	6	65	theme	neural	954:959	arg1	structure					974:982	neural and vascular structure	954:982	neural and vascular structure	954:982	The composite not only inhibits death of neuro- and vascular cells and leads to the preservation of neural and vascular structure, but also modulates the inflammatory phenotype of macrophages in the locally injured region.					
35333441	0	66	with	Tissue	34:39	arg1	Hydrogel					65:72	a Hyaluronan-Based Hydrogel	46:72	a Hyaluronan-Based Hydrogel	46:72	Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.					
35333441	4	67	theme	human	665:669	arg1	tissue					675:680	human fat tissue	665:680	human fat tissue using mechanical methods	665:705	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	6	68	theme	neuro-	895:900	arg1	cells					915:919	neuro- and vascular cells	895:919	neuro- and vascular cells	895:919	The composite not only inhibits death of neuro- and vascular cells and leads to the preservation of neural and vascular structure, but also modulates the inflammatory phenotype of macrophages in the locally injured region.					
35333441	4	69	theme	cell-free	589:597	arg1	component					606:614	a cell-free liquid component	587:614	a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods	587:705	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
35333441	4	69	theme	cell-free	589:597	arg1	extract					571:577	Fat extract	567:577	Fat extract (FE)	567:582	Fat extract (FE) is a cell-free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods.					
37100359	0	0	theme	mechanical	67:76	arg1	properties					78:87	mechanical properties	67:87	mechanical properties	67:87	Loss of hyaluronan synthases impacts bone morphology, quality, and mechanical properties.					
37100359	3	1	theme	confocal	595:602	arg1	spectroscopy					610:621	confocal Raman spectroscopy	595:621	confocal Raman spectroscopy	595:621	Femora were isolated from Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice and were analyzed using microcomputed-tomography, confocal Raman spectroscopy, three-point bending, and nanoindentation.					
37100359	7	2	theme	bone	1319:1322	arg1	structure					1324:1332	cortical bone structure	1310:1332	cortical bone structure	1310:1332	Taken together, these results demonstrate, for the first time, the impact of the loss of hyaluronan synthase isoforms on cortical bone structure, content, and biomechanics.					
37100359	8	3	theme	whole	1538:1542	arg1	mechanics					1549:1557	whole bone mechanics	1538:1557	whole bone mechanics	1538:1557	Loss of Has1 impacted morphology, mineralization, and micron-level hardness, while loss of Has3 reduced bone mineral density and affected organic matrix composition, impacting whole bone mechanics.					
37100359	6	4	theme	glycation	1144:1152	arg1	end-products					1154:1165	advanced glycation end-products	1135:1165	advanced glycation end-products than WT (p = 0.0478)	1135:1186	Interestingly, loss of Has3 was also associated with significantly lower accumulation of advanced glycation end-products than WT (p = 0.0478).					
37100359	7	5	theme	first	1240:1244	arg1	time					1246:1249	the first time	1236:1249	the first time	1236:1249	Taken together, these results demonstrate, for the first time, the impact of the loss of hyaluronan synthase isoforms on cortical bone structure, content, and biomechanics.					
37100359	8	6	theme	Has1	1370:1373	arg1	Loss					1362:1365	Loss	1362:1365	Loss of Has1	1362:1373	Loss of Has1 impacted morphology, mineralization, and micron-level hardness, while loss of Has3 reduced bone mineral density and affected organic matrix composition, impacting whole bone mechanics.					
37100359	9	7	theme	loss	1614:1617	arg1	effect					1604:1609	the effect	1600:1609	the effect of loss of hyaluronan synthases on bone quality	1600:1657	This is the first study to characterize the effect of loss of hyaluronan synthases on bone quality, suggesting an essential role hyaluronan plays during the development and regulation of bone.					
37100359	9	8	theme	bone	1747:1750	arg1	development					1717:1727	development	1717:1727	development	1717:1727	This is the first study to characterize the effect of loss of hyaluronan synthases on bone quality, suggesting an essential role hyaluronan plays during the development and regulation of bone.					
37100359	9	8	theme	bone	1747:1750	arg1	regulation					1733:1742	regulation	1733:1742	regulation	1733:1742	This is the first study to characterize the effect of loss of hyaluronan synthases on bone quality, suggesting an essential role hyaluronan plays during the development and regulation of bone.					
37100359	2	9	theme	overall	429:435	arg1	strength					437:444	overall strength	429:444	overall strength	429:444	The goal of this study is to characterize how the loss of Has1 or Has3 affects the morphology, matrix properties, and overall strength of murine bone.					
37100359	1	10	theme	bone	284:287	arg1	quality					289:295	bone quality	284:295	bone quality	284:295	Hyaluronan, a glycosaminoglycan synthesized by three isoenzymes (Has1, Has2, Has3), is known to play a role in regulating bone turnover, remodeling, and mineralization, which in turn can affect bone quality and strength.					
37100359	5	11	theme	higher	923:928	arg1	p < 0.0001					955:964	p < 0.0001	955:964	p < 0.0001	955:964	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	5	11	theme	higher	923:928	arg1	ratio					948:952	higher mineral-to-matrix ratio	923:952	higher mineral-to-matrix ratio (p < 0.0001)	923:965	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	5	12	theme	Has3-/-	857:863	arg1	bones					865:869	Has3-/- bones	857:869	Has3-/- bones	857:869	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	1	13	theme	bone	212:215	arg1	turnover					217:224	bone turnover	212:224	bone turnover	212:224	Hyaluronan, a glycosaminoglycan synthesized by three isoenzymes (Has1, Has2, Has3), is known to play a role in regulating bone turnover, remodeling, and mineralization, which in turn can affect bone quality and strength.					
37100359	9	14	theme	synthases	1633:1641	arg1	loss					1614:1617	loss	1614:1617	loss of hyaluronan synthases	1614:1641	This is the first study to characterize the effect of loss of hyaluronan synthases on bone quality, suggesting an essential role hyaluronan plays during the development and regulation of bone.					
37100359	4	15	theme	reduced	779:785	arg1	p = 0.033					797:805	p = 0.033	797:805	p = 0.033	797:805	Of the three genotypes tested, Has1-/- bones demonstrated significantly lower cross-sectional area (p = 0.0002), reduced hardness (p = 0.033), and lower mineral-to-matrix ratio (p < 0.0001).					
37100359	4	15	theme	reduced	779:785	arg1	hardness					787:794	reduced hardness	779:794	reduced hardness (p = 0.033)	779:806	Of the three genotypes tested, Has1-/- bones demonstrated significantly lower cross-sectional area (p = 0.0002), reduced hardness (p = 0.033), and lower mineral-to-matrix ratio (p < 0.0001).					
37100359	3	16	attach	isolated	474:481	arg2	Femora					462:467	Femora	462:467	Femora	462:467	Femora were isolated from Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice and were analyzed using microcomputed-tomography, confocal Raman spectroscopy, three-point bending, and nanoindentation.					
37100359	3	16	attach	isolated	474:481	arg1	mice					540:543	Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice	488:543	Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice	488:543	Femora were isolated from Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice and were analyzed using microcomputed-tomography, confocal Raman spectroscopy, three-point bending, and nanoindentation.					
37100359	8	17	theme	affected	1491:1498	arg1	composition					1515:1525	affected organic matrix composition	1491:1525	affected organic matrix composition	1491:1525	Loss of Has1 impacted morphology, mineralization, and micron-level hardness, while loss of Has3 reduced bone mineral density and affected organic matrix composition, impacting whole bone mechanics.					
37100359	3	18	theme	three-point	624:634	arg1	bending					636:642	three-point bending	624:642	three-point bending	624:642	Femora were isolated from Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice and were analyzed using microcomputed-tomography, confocal Raman spectroscopy, three-point bending, and nanoindentation.					
37100359	6	19	theme	end-products	1154:1165	arg1	accumulation					1119:1130	significantly lower accumulation	1099:1130	significantly lower accumulation of advanced glycation end-products than WT (p = 0.0478)	1099:1186	Interestingly, loss of Has3 was also associated with significantly lower accumulation of advanced glycation end-products than WT (p = 0.0478).					
37100359	7	20	theme	hyaluronan	1278:1287	arg1	synthase					1289:1296	hyaluronan synthase	1278:1296	hyaluronan synthase isoforms	1278:1305	Taken together, these results demonstrate, for the first time, the impact of the loss of hyaluronan synthase isoforms on cortical bone structure, content, and biomechanics.					
37100359	5	21	contain	had	871:873	arg1	bones					865:869	Has3-/- bones	857:869	Has3-/- bones	857:869	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	5	21	contain	had	871:873	arg2	p < 0.0001					955:964	p < 0.0001	955:964	p < 0.0001	955:964	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	5	21	contain	had	871:873	arg2	p < 0.0001					907:916	p < 0.0001	907:916	p < 0.0001	907:916	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	5	21	contain	had	871:873	arg2	stiffness					896:904	significantly higher stiffness	875:904	significantly higher stiffness (p < 0.0001)	875:917	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	5	21	contain	had	871:873	arg2	ratio					948:952	higher mineral-to-matrix ratio	923:952	higher mineral-to-matrix ratio (p < 0.0001)	923:965	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	9	22	theme	bone	1646:1649	arg1	quality					1651:1657	bone quality	1646:1657	bone quality	1646:1657	This is the first study to characterize the effect of loss of hyaluronan synthases on bone quality, suggesting an essential role hyaluronan plays during the development and regulation of bone.					
37100359	3	23	theme	Raman	604:608	arg1	spectroscopy					610:621	confocal Raman spectroscopy	595:621	confocal Raman spectroscopy	595:621	Femora were isolated from Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice and were analyzed using microcomputed-tomography, confocal Raman spectroscopy, three-point bending, and nanoindentation.					
37100359	4	24	theme	cross-sectional	744:758	arg1	p = 0.0002					766:775	p = 0.0002	766:775	p = 0.0002	766:775	Of the three genotypes tested, Has1-/- bones demonstrated significantly lower cross-sectional area (p = 0.0002), reduced hardness (p = 0.033), and lower mineral-to-matrix ratio (p < 0.0001).					
37100359	4	24	theme	cross-sectional	744:758	arg1	area					760:763	significantly lower cross-sectional area	724:763	significantly lower cross-sectional area (p = 0.0002)	724:776	Of the three genotypes tested, Has1-/- bones demonstrated significantly lower cross-sectional area (p = 0.0002), reduced hardness (p = 0.033), and lower mineral-to-matrix ratio (p < 0.0001).					
37100359	7	25	theme	synthase	1289:1296	arg1	isoforms					1298:1305	hyaluronan synthase isoforms	1278:1305	hyaluronan synthase isoforms	1278:1305	Taken together, these results demonstrate, for the first time, the impact of the loss of hyaluronan synthase isoforms on cortical bone structure, content, and biomechanics.					
37100359	3	26	dep	Has1-/-	488:494	arg1	WT					519:520	WT	519:520	WT	519:520	Femora were isolated from Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice and were analyzed using microcomputed-tomography, confocal Raman spectroscopy, three-point bending, and nanoindentation.					
37100359	5	27	theme	bone	1003:1006	arg1	density					1016:1022	bone mineral density	1003:1022	bone mineral density (p < 0.0001)	1003:1035	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	5	27	theme	bone	1003:1006	arg1	p < 0.0001					1025:1034	p < 0.0001	1025:1034	p < 0.0001	1025:1034	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	6	28	theme	advanced	1135:1142	arg1	end-products					1154:1165	advanced glycation end-products	1135:1165	advanced glycation end-products than WT (p = 0.0478)	1135:1186	Interestingly, loss of Has3 was also associated with significantly lower accumulation of advanced glycation end-products than WT (p = 0.0478).					
37100359	7	29	theme	loss	1270:1273	arg1	impact					1256:1261	the impact	1252:1261	the impact of the loss of hyaluronan synthase isoforms on cortical bone structure, content, and biomechanics	1252:1359	Taken together, these results demonstrate, for the first time, the impact of the loss of hyaluronan synthase isoforms on cortical bone structure, content, and biomechanics.					
37100359	9	30	theme	first	1572:1576	arg1	This					1560:1563	This	1560:1563	This	1560:1563	This is the first study to characterize the effect of loss of hyaluronan synthases on bone quality, suggesting an essential role hyaluronan plays during the development and regulation of bone.					
37100359	9	30	theme	first	1572:1576	arg1	study					1578:1582	the first study	1568:1582	the first study to characterize the effect of loss of hyaluronan synthases on bone quality	1568:1657	This is the first study to characterize the effect of loss of hyaluronan synthases on bone quality, suggesting an essential role hyaluronan plays during the development and regulation of bone.					
37100359	9	31	dep	role	1684:1687	arg1	plays					1700:1704	plays	1700:1704	plays during the development and regulation of bone	1700:1750	This is the first study to characterize the effect of loss of hyaluronan synthases on bone quality, suggesting an essential role hyaluronan plays during the development and regulation of bone.					
37100359	0	32	theme	synthases	19:27	arg1	Loss					0:3	Loss	0:3	Loss of hyaluronan synthases	0:27	Loss of hyaluronan synthases impacts bone morphology, quality, and mechanical properties.					
37100359	2	33	theme	bone	456:459	arg1	strength					437:444	overall strength	429:444	overall strength	429:444	The goal of this study is to characterize how the loss of Has1 or Has3 affects the morphology, matrix properties, and overall strength of murine bone.					
37100359	2	33	theme	bone	456:459	arg1	morphology					394:403	morphology	394:403	morphology	394:403	The goal of this study is to characterize how the loss of Has1 or Has3 affects the morphology, matrix properties, and overall strength of murine bone.					
37100359	2	33	theme	bone	456:459	arg1	properties					413:422	matrix properties	406:422	matrix properties	406:422	The goal of this study is to characterize how the loss of Has1 or Has3 affects the morphology, matrix properties, and overall strength of murine bone.					
37100359	5	34	theme	mineral-to-matrix	930:946	arg1	p < 0.0001					955:964	p < 0.0001	955:964	p < 0.0001	955:964	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	5	34	theme	mineral-to-matrix	930:946	arg1	ratio					948:952	higher mineral-to-matrix ratio	923:952	higher mineral-to-matrix ratio (p < 0.0001)	923:965	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	9	35	dep	development	1717:1727	arg1	the					1713:1715	the	1713:1715	the	1713:1715	This is the first study to characterize the effect of loss of hyaluronan synthases on bone quality, suggesting an essential role hyaluronan plays during the development and regulation of bone.					
37100359	0	36	theme	hyaluronan	8:17	arg1	synthases					19:27	hyaluronan synthases	8:27	hyaluronan synthases	8:27	Loss of hyaluronan synthases impacts bone morphology, quality, and mechanical properties.					
37100359	2	37	theme	murine	449:454	arg1	bone					456:459	murine bone	449:459	murine bone	449:459	The goal of this study is to characterize how the loss of Has1 or Has3 affects the morphology, matrix properties, and overall strength of murine bone.					
37100359	3	38	theme	C57Bl/6 J	523:531	arg1	mice					540:543	Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice	488:543	Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice	488:543	Femora were isolated from Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice and were analyzed using microcomputed-tomography, confocal Raman spectroscopy, three-point bending, and nanoindentation.					
37100359	1	39	dep	isoenzymes	143:152	arg1	Has3					167:170	Has3	167:170	Has3	167:170	Hyaluronan, a glycosaminoglycan synthesized by three isoenzymes (Has1, Has2, Has3), is known to play a role in regulating bone turnover, remodeling, and mineralization, which in turn can affect bone quality and strength.					
37100359	1	39	dep	isoenzymes	143:152	arg1	Has2					161:164	Has2	161:164	Has2	161:164	Hyaluronan, a glycosaminoglycan synthesized by three isoenzymes (Has1, Has2, Has3), is known to play a role in regulating bone turnover, remodeling, and mineralization, which in turn can affect bone quality and strength.					
37100359	1	39	dep	isoenzymes	143:152	arg1	Has1					155:158	Has1	155:158	Has1	155:158	Hyaluronan, a glycosaminoglycan synthesized by three isoenzymes (Has1, Has2, Has3), is known to play a role in regulating bone turnover, remodeling, and mineralization, which in turn can affect bone quality and strength.					
37100359	0	40	theme	bone	37:40	arg1	morphology					42:51	bone morphology	37:51	bone morphology	37:51	Loss of hyaluronan synthases impacts bone morphology, quality, and mechanical properties.					
37100359	8	41	theme	micron-level	1416:1427	arg1	hardness					1429:1436	micron-level hardness	1416:1436	micron-level hardness	1416:1436	Loss of Has1 impacted morphology, mineralization, and micron-level hardness, while loss of Has3 reduced bone mineral density and affected organic matrix composition, impacting whole bone mechanics.					
37100359	3	42	theme	female	533:538	arg1	mice					540:543	Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice	488:543	Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice	488:543	Femora were isolated from Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice and were analyzed using microcomputed-tomography, confocal Raman spectroscopy, three-point bending, and nanoindentation.					
37100359	7	43	from	impact	1256:1261	arg1	structure					1324:1332	cortical bone structure	1310:1332	cortical bone structure	1310:1332	Taken together, these results demonstrate, for the first time, the impact of the loss of hyaluronan synthase isoforms on cortical bone structure, content, and biomechanics.					
37100359	7	43	from	impact	1256:1261	arg1	content					1335:1341	content	1335:1341	content	1335:1341	Taken together, these results demonstrate, for the first time, the impact of the loss of hyaluronan synthase isoforms on cortical bone structure, content, and biomechanics.					
37100359	7	43	from	impact	1256:1261	arg1	biomechanics					1348:1359	biomechanics	1348:1359	biomechanics	1348:1359	Taken together, these results demonstrate, for the first time, the impact of the loss of hyaluronan synthase isoforms on cortical bone structure, content, and biomechanics.					
37100359	2	44	theme	Has3	377:380	arg1	loss					361:364	the loss	357:364	the loss of Has1 or Has3	357:380	The goal of this study is to characterize how the loss of Has1 or Has3 affects the morphology, matrix properties, and overall strength of murine bone.					
37100359	4	45	theme	mineral-to-matrix	819:835	arg1	p < 0.0001					844:853	p < 0.0001	844:853	p < 0.0001	844:853	Of the three genotypes tested, Has1-/- bones demonstrated significantly lower cross-sectional area (p = 0.0002), reduced hardness (p = 0.033), and lower mineral-to-matrix ratio (p < 0.0001).					
37100359	4	45	theme	mineral-to-matrix	819:835	arg1	ratio					837:841	lower mineral-to-matrix ratio	813:841	lower mineral-to-matrix ratio (p < 0.0001)	813:854	Of the three genotypes tested, Has1-/- bones demonstrated significantly lower cross-sectional area (p = 0.0002), reduced hardness (p = 0.033), and lower mineral-to-matrix ratio (p < 0.0001).					
37100359	3	46	theme	Has3-/-	496:502	arg1	mice					540:543	Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice	488:543	Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice	488:543	Femora were isolated from Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice and were analyzed using microcomputed-tomography, confocal Raman spectroscopy, three-point bending, and nanoindentation.					
37100359	2	47	theme	study	328:332	arg1	goal					315:318	The goal	311:318	The goal of this study	311:332	The goal of this study is to characterize how the loss of Has1 or Has3 affects the morphology, matrix properties, and overall strength of murine bone.					
37100359	9	48	theme	hyaluronan	1622:1631	arg1	synthases					1633:1641	hyaluronan synthases	1622:1641	hyaluronan synthases	1622:1641	This is the first study to characterize the effect of loss of hyaluronan synthases on bone quality, suggesting an essential role hyaluronan plays during the development and regulation of bone.					
37100359	2	49	dep	morphology	394:403	arg1	the					390:392	the	390:392	the	390:392	The goal of this study is to characterize how the loss of Has1 or Has3 affects the morphology, matrix properties, and overall strength of murine bone.					
37100359	4	50	theme	lower	738:742	arg1	p = 0.0002					766:775	p = 0.0002	766:775	p = 0.0002	766:775	Of the three genotypes tested, Has1-/- bones demonstrated significantly lower cross-sectional area (p = 0.0002), reduced hardness (p = 0.033), and lower mineral-to-matrix ratio (p < 0.0001).					
37100359	4	50	theme	lower	738:742	arg1	area					760:763	significantly lower cross-sectional area	724:763	significantly lower cross-sectional area (p = 0.0002)	724:776	Of the three genotypes tested, Has1-/- bones demonstrated significantly lower cross-sectional area (p = 0.0002), reduced hardness (p = 0.033), and lower mineral-to-matrix ratio (p < 0.0001).					
37100359	7	51	theme	cortical	1310:1317	arg1	structure					1324:1332	cortical bone structure	1310:1332	cortical bone structure	1310:1332	Taken together, these results demonstrate, for the first time, the impact of the loss of hyaluronan synthase isoforms on cortical bone structure, content, and biomechanics.					
37100359	2	52	theme	Has1	369:372	arg1	loss					361:364	the loss	357:364	the loss of Has1 or Has3	357:380	The goal of this study is to characterize how the loss of Has1 or Has3 affects the morphology, matrix properties, and overall strength of murine bone.					
37100359	4	53	theme	lower	813:817	arg1	p < 0.0001					844:853	p < 0.0001	844:853	p < 0.0001	844:853	Of the three genotypes tested, Has1-/- bones demonstrated significantly lower cross-sectional area (p = 0.0002), reduced hardness (p = 0.033), and lower mineral-to-matrix ratio (p < 0.0001).					
37100359	4	53	theme	lower	813:817	arg1	ratio					837:841	lower mineral-to-matrix ratio	813:841	lower mineral-to-matrix ratio (p < 0.0001)	813:854	Of the three genotypes tested, Has1-/- bones demonstrated significantly lower cross-sectional area (p = 0.0002), reduced hardness (p = 0.033), and lower mineral-to-matrix ratio (p < 0.0001).					
37100359	3	54	theme	Has1-/-	488:494	arg1	mice					540:543	Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice	488:543	Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice	488:543	Femora were isolated from Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice and were analyzed using microcomputed-tomography, confocal Raman spectroscopy, three-point bending, and nanoindentation.					
37100359	5	55	theme	higher	889:894	arg1	p < 0.0001					907:916	p < 0.0001	907:916	p < 0.0001	907:916	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	5	55	theme	higher	889:894	arg1	stiffness					896:904	significantly higher stiffness	875:904	significantly higher stiffness (p < 0.0001)	875:917	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	3	56	theme	wildtype	509:516	arg1	mice					540:543	Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice	488:543	Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice	488:543	Femora were isolated from Has1-/-,Has3-/-, and wildtype (WT) C57Bl/6 J female mice and were analyzed using microcomputed-tomography, confocal Raman spectroscopy, three-point bending, and nanoindentation.					
37100359	6	57	theme	lower	1113:1117	arg1	accumulation					1119:1130	significantly lower accumulation	1099:1130	significantly lower accumulation of advanced glycation end-products than WT (p = 0.0478)	1099:1186	Interestingly, loss of Has3 was also associated with significantly lower accumulation of advanced glycation end-products than WT (p = 0.0478).					
37100359	8	58	theme	matrix	1508:1513	arg1	composition					1515:1525	affected organic matrix composition	1491:1525	affected organic matrix composition	1491:1525	Loss of Has1 impacted morphology, mineralization, and micron-level hardness, while loss of Has3 reduced bone mineral density and affected organic matrix composition, impacting whole bone mechanics.					
37100359	5	59	dep	strength	977:984	arg1	lower					971:975	lower	971:975	lower	971:975	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	9	60	from	effect	1604:1609	arg1	quality					1651:1657	bone quality	1646:1657	bone quality	1646:1657	This is the first study to characterize the effect of loss of hyaluronan synthases on bone quality, suggesting an essential role hyaluronan plays during the development and regulation of bone.					
37100359	7	61	theme	isoforms	1298:1305	arg1	loss					1270:1273	the loss	1266:1273	the loss of hyaluronan synthase isoforms	1266:1305	Taken together, these results demonstrate, for the first time, the impact of the loss of hyaluronan synthase isoforms on cortical bone structure, content, and biomechanics.					
37100359	9	62	theme	essential	1674:1682	arg1	role					1684:1687	an essential role	1671:1687	an essential role hyaluronan plays during the development and regulation of bone	1671:1750	This is the first study to characterize the effect of loss of hyaluronan synthases on bone quality, suggesting an essential role hyaluronan plays during the development and regulation of bone.					
37100359	8	63	theme	Has3	1453:1456	arg1	loss					1445:1448	loss	1445:1448	loss of Has3	1445:1456	Loss of Has1 impacted morphology, mineralization, and micron-level hardness, while loss of Has3 reduced bone mineral density and affected organic matrix composition, impacting whole bone mechanics.					
37100359	2	64	theme	matrix	406:411	arg1	properties					413:422	matrix properties	406:422	matrix properties	406:422	The goal of this study is to characterize how the loss of Has1 or Has3 affects the morphology, matrix properties, and overall strength of murine bone.					
37100359	4	65	theme	Has1-/-	697:703	arg1	bones					705:709	Has1-/- bones	697:709	Has1-/- bones	697:709	Of the three genotypes tested, Has1-/- bones demonstrated significantly lower cross-sectional area (p = 0.0002), reduced hardness (p = 0.033), and lower mineral-to-matrix ratio (p < 0.0001).					
37100359	8	66	theme	mineral	1471:1477	arg1	density					1479:1485	bone mineral density	1466:1485	bone mineral density	1466:1485	Loss of Has1 impacted morphology, mineralization, and micron-level hardness, while loss of Has3 reduced bone mineral density and affected organic matrix composition, impacting whole bone mechanics.					
37100359	8	67	theme	organic	1500:1506	arg1	composition					1515:1525	affected organic matrix composition	1491:1525	affected organic matrix composition	1491:1525	Loss of Has1 impacted morphology, mineralization, and micron-level hardness, while loss of Has3 reduced bone mineral density and affected organic matrix composition, impacting whole bone mechanics.					
37100359	5	68	theme	mineral	1008:1014	arg1	density					1016:1022	bone mineral density	1003:1022	bone mineral density (p < 0.0001)	1003:1035	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	5	68	theme	mineral	1008:1014	arg1	p < 0.0001					1025:1034	p < 0.0001	1025:1034	p < 0.0001	1025:1034	Has3-/- bones had significantly higher stiffness (p < 0.0001) and higher mineral-to-matrix ratio (p < 0.0001) but lower strength (p = 0.0014) and bone mineral density (p < 0.0001) than WT.					
37100359	6	69	theme	Has3	1069:1072	arg1	loss					1061:1064	loss	1061:1064	loss of Has3	1061:1072	Interestingly, loss of Has3 was also associated with significantly lower accumulation of advanced glycation end-products than WT (p = 0.0478).					
37100359	8	70	theme	bone	1544:1547	arg1	mechanics					1549:1557	whole bone mechanics	1538:1557	whole bone mechanics	1538:1557	Loss of Has1 impacted morphology, mineralization, and micron-level hardness, while loss of Has3 reduced bone mineral density and affected organic matrix composition, impacting whole bone mechanics.					
37100359	8	71	theme	bone	1466:1469	arg1	density					1479:1485	bone mineral density	1466:1485	bone mineral density	1466:1485	Loss of Has1 impacted morphology, mineralization, and micron-level hardness, while loss of Has3 reduced bone mineral density and affected organic matrix composition, impacting whole bone mechanics.					
36481513	8	0	theme	protein	1560:1566	arg1	pH					1568:1569	whey protein pH	1555:1569	whey protein pH	1555:1569	The encapsulates prepared without adjusting whey protein pH showed the least release (∼51 % in 24 h) and bioaccessibility (∼56%) of iron indicating the iron-whey protein complex formation.					
36481513	6	1	theme	lightness	1198:1206	arg1	60.80 ± 0.32					1215:1226	60.80 ± 0.32	1215:1226	60.80 ± 0.32	1215:1226	Whereas, encapsulates having HPMC as shell polymer showed highest lightness value (60.80 ± 0.32) and highest encapsulation efficiency for iron (87.28 ± 4.15%).					
36481513	6	1	theme	lightness	1198:1206	arg1	value					1208:1212	highest lightness value	1190:1212	highest lightness value (60.80 ± 0.32)	1190:1227	Whereas, encapsulates having HPMC as shell polymer showed highest lightness value (60.80 ± 0.32) and highest encapsulation efficiency for iron (87.28 ± 4.15%).					
36481513	2	2	theme	ascorbic	338:345	arg1	acid					347:350	ascorbic acid	338:350	ascorbic acid	338:350	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	7	3	theme	reduced	1398:1404	arg1	bonds					1412:1416	reduced amine bonds	1398:1416	reduced amine bonds	1398:1416	The formation of core-shell structure was confirmed by evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying.					
36481513	5	4	with	flowability	969:979	arg1	values					993:998	lowest values	986:998	lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71)	986:1061	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	9	5	theme	whey	1800:1803	arg1	protein					1805:1811	whey protein	1800:1811	whey protein	1800:1811	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	6	6	theme	shell	1169:1173	arg1	polymer					1175:1181	shell polymer	1169:1181	shell polymer	1169:1181	Whereas, encapsulates having HPMC as shell polymer showed highest lightness value (60.80 ± 0.32) and highest encapsulation efficiency for iron (87.28 ± 4.15%).					
36481513	7	7	theme	surface	1365:1371	arg1	composition					1373:1383	the surface composition	1361:1383	the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying	1361:1508	The formation of core-shell structure was confirmed by evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying.					
36481513	7	8	theme	structure	1320:1328	arg1	formation					1296:1304	The formation	1292:1304	The formation of core-shell structure	1292:1328	The formation of core-shell structure was confirmed by evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying.					
36481513	5	9	theme	Hausner	1003:1009	arg1	ratio					1011:1015	Hausner ratio	1003:1015	Hausner ratio (1.25 ± 0.04)	1003:1029	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	5	9	theme	Hausner	1003:1009	arg1	1.25 ± 0.04					1018:1028	1.25 ± 0.04	1018:1028	1.25 ± 0.04	1018:1028	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	2	10	theme	scalable	292:299	arg1	approach					301:308	a scalable approach	290:308	a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers	290:538	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	4	11	dep	pH	770:771	arg1	to					773:774	to	773:774	to	773:774	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	4	12	theme	native	879:884	arg1	38.91 ± 0.58					890:901	38.91 ± 0.58	890:901	38.91 ± 0.58	890:901	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	4	12	theme	native	879:884	arg1	pH					886:887	native pH	879:887	native pH (38.91 ± 0.58)	879:902	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	5	13	with	sample	918:923	arg1	pectin					930:935	pectin	930:935	pectin	930:935	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	6	14	theme	highest	1233:1239	arg1	%					1288:1288	87.28 ± 4.15%	1276:1288	87.28 ± 4.15%	1276:1288	Whereas, encapsulates having HPMC as shell polymer showed highest lightness value (60.80 ± 0.32) and highest encapsulation efficiency for iron (87.28 ± 4.15%).					
36481513	6	14	theme	highest	1233:1239	arg1	efficiency					1255:1264	highest encapsulation efficiency	1233:1264	highest encapsulation efficiency for iron (87.28 ± 4.15%)	1233:1289	Whereas, encapsulates having HPMC as shell polymer showed highest lightness value (60.80 ± 0.32) and highest encapsulation efficiency for iron (87.28 ± 4.15%).					
36481513	3	15	theme	formation	561:569	arg1	effect					545:550	The effect	541:550	The effect of shell formation	541:569	The effect of shell formation was observed by comparing core-shell encapsulates with conventional 2-fluid nozzle (2FN) encapsulates.					
36481513	8	16	theme	iron-whey	1663:1671	arg1	complex					1681:1687	the iron-whey protein complex	1659:1687	the iron-whey protein complex formation	1659:1697	The encapsulates prepared without adjusting whey protein pH showed the least release (∼51 % in 24 h) and bioaccessibility (∼56%) of iron indicating the iron-whey protein complex formation.					
36481513	1	17	theme	iron	122:125	arg1	concern					111:117	the growing concern	99:117	the growing concern of iron and folic acid deficiency	99:151	Considering the growing concern of iron and folic acid deficiency, encapsulation of these nutrients and fortification into foods is emerging as an effective counter-strategy.					
36481513	8	18	from	%	1601:1601	arg1	24 h					1606:1609	24 h	1606:1609	24 h	1606:1609	The encapsulates prepared without adjusting whey protein pH showed the least release (∼51 % in 24 h) and bioaccessibility (∼56%) of iron indicating the iron-whey protein complex formation.					
36481513	8	19	theme	complex	1681:1687	arg1	formation					1689:1697	the iron-whey protein complex formation	1659:1697	the iron-whey protein complex formation	1659:1697	The encapsulates prepared without adjusting whey protein pH showed the least release (∼51 % in 24 h) and bioaccessibility (∼56%) of iron indicating the iron-whey protein complex formation.					
36481513	1	20	theme	folic	131:135	arg1	acid					137:140	folic acid	131:140	folic acid deficiency	131:151	Considering the growing concern of iron and folic acid deficiency, encapsulation of these nutrients and fortification into foods is emerging as an effective counter-strategy.					
36481513	8	21	dep	release	1588:1594	arg1	%					1601:1601	∼51 %	1597:1601	∼51 % in 24 h	1597:1609	The encapsulates prepared without adjusting whey protein pH showed the least release (∼51 % in 24 h) and bioaccessibility (∼56%) of iron indicating the iron-whey protein complex formation.					
36481513	0	22	theme	iron-vitamin	15:26	arg1	encapsulates					39:50	iron-vitamin multilayer encapsulates	15:50	iron-vitamin multilayer encapsulates using 3 fluid	15:64	Development of iron-vitamin multilayer encapsulates using 3 fluid nozzle spray drying.					
36481513	1	23	theme	deficiency	142:151	arg1	concern					111:117	the growing concern	99:117	the growing concern of iron and folic acid deficiency	99:151	Considering the growing concern of iron and folic acid deficiency, encapsulation of these nutrients and fortification into foods is emerging as an effective counter-strategy.					
36481513	9	24	theme	ascorbic	1884:1891	arg1	acid					1893:1896	ascorbic acid	1884:1896	ascorbic acid	1884:1896	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	9	24	theme	ascorbic	1884:1891	arg1	co-encapsulation					1843:1858	co-encapsulation	1843:1858	co-encapsulation of iron	1843:1866	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	7	25	theme	3FN	1493:1495	arg1	drying					1503:1508	3FN spray drying	1493:1508	3FN spray drying	1493:1508	The formation of core-shell structure was confirmed by evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying.					
36481513	2	26	theme	encapsulates	379:390	arg1	production					318:327	the production	314:327	the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers	314:538	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	4	27	with	encapsulates	861:872	arg1	38.91 ± 0.58					890:901	38.91 ± 0.58	890:901	38.91 ± 0.58	890:901	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	4	27	with	encapsulates	861:872	arg1	pH					886:887	native pH	879:887	native pH (38.91 ± 0.58)	879:902	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	2	28	theme	spray	425:429	arg1	polymers					531:538	shell polymers	525:538	shell polymers	525:538	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	2	28	theme	spray	425:429	arg1	drying					431:436	novel 3-fluid nozzle (3FN) spray drying	398:436	novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC)	398:520	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	2	29	theme	acid	363:366	arg1	encapsulates					379:390	iron, ascorbic acid, and folic acid core-shell encapsulates	332:390	iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers	332:538	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	2	30	theme	3FN	420:422	arg1	polymers					531:538	shell polymers	525:538	shell polymers	525:538	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	2	30	theme	3FN	420:422	arg1	drying					431:436	novel 3-fluid nozzle (3FN) spray drying	398:436	novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC)	398:520	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	4	31	theme	protein	705:711	arg1	pH					694:695	pH	694:695	pH of whey protein	694:711	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	2	32	theme	nozzle	412:417	arg1	polymers					531:538	shell polymers	525:538	shell polymers	525:538	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	2	32	theme	nozzle	412:417	arg1	drying					431:436	novel 3-fluid nozzle (3FN) spray drying	398:436	novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC)	398:520	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	8	33	theme	protein	1673:1679	arg1	complex					1681:1687	the iron-whey protein complex	1659:1687	the iron-whey protein complex formation	1659:1697	The encapsulates prepared without adjusting whey protein pH showed the least release (∼51 % in 24 h) and bioaccessibility (∼56%) of iron indicating the iron-whey protein complex formation.					
36481513	9	34	theme	smooth	1721:1726	arg1	morphology					1736:1745	smooth surface morphology	1721:1745	smooth surface morphology	1721:1745	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	6	35	contain	having	1154:1159	arg2	HPMC					1161:1164	HPMC	1161:1164	HPMC	1161:1164	Whereas, encapsulates having HPMC as shell polymer showed highest lightness value (60.80 ± 0.32) and highest encapsulation efficiency for iron (87.28 ± 4.15%).					
36481513	6	35	contain	having	1154:1159	arg1	encapsulates					1141:1152	encapsulates	1141:1152	encapsulates having HPMC as shell polymer	1141:1181	Whereas, encapsulates having HPMC as shell polymer showed highest lightness value (60.80 ± 0.32) and highest encapsulation efficiency for iron (87.28 ± 4.15%).					
36481513	3	36	theme	conventional	626:637	arg1	encapsulates					660:671	conventional 2-fluid nozzle (2FN) encapsulates	626:671	conventional 2-fluid nozzle (2FN) encapsulates	626:671	The effect of shell formation was observed by comparing core-shell encapsulates with conventional 2-fluid nozzle (2FN) encapsulates.					
36481513	2	37	theme	novel	398:402	arg1	polymers					531:538	shell polymers	525:538	shell polymers	525:538	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	2	37	theme	novel	398:402	arg1	drying					431:436	novel 3-fluid nozzle (3FN) spray drying	398:436	novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC)	398:520	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	0	38	theme	encapsulates	39:50	arg1	Development					0:10	Development	0:10	Development of iron-vitamin multilayer encapsulates using 3 fluid	0:64	Development of iron-vitamin multilayer encapsulates using 3 fluid nozzle spray drying.					
36481513	9	39	theme	surface	1728:1734	arg1	morphology					1736:1745	smooth surface morphology	1721:1745	smooth surface morphology	1721:1745	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	7	40	theme	carbonyl	1446:1453	arg1	bonds					1455:1459	increased aliphatic and carbonyl bonds	1422:1459	increased aliphatic and carbonyl bonds	1422:1459	The formation of core-shell structure was confirmed by evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying.					
36481513	5	41	theme	encapsulation	1075:1087	arg1	efficiency					1089:1098	highest encapsulation efficiency	1067:1098	highest encapsulation efficiency for folic acid (86.07 ± 5.24%)	1067:1129	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	7	42	theme	aliphatic	1432:1440	arg1	bonds					1455:1459	increased aliphatic and carbonyl bonds	1422:1459	increased aliphatic and carbonyl bonds	1422:1459	The formation of core-shell structure was confirmed by evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying.					
36481513	3	43	theme	2FN	655:657	arg1	encapsulates					660:671	conventional 2-fluid nozzle (2FN) encapsulates	626:671	conventional 2-fluid nozzle (2FN) encapsulates	626:671	The effect of shell formation was observed by comparing core-shell encapsulates with conventional 2-fluid nozzle (2FN) encapsulates.					
36481513	4	44	from	effect	684:689	arg1	color					720:724	the color	716:724	the color of encapsulates	716:740	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	3	45	theme	nozzle	647:652	arg1	encapsulates					660:671	conventional 2-fluid nozzle (2FN) encapsulates	626:671	conventional 2-fluid nozzle (2FN) encapsulates	626:671	The effect of shell formation was observed by comparing core-shell encapsulates with conventional 2-fluid nozzle (2FN) encapsulates.					
36481513	7	46	theme	amine	1406:1410	arg1	bonds					1412:1416	reduced amine bonds	1398:1416	reduced amine bonds	1398:1416	The formation of core-shell structure was confirmed by evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying.					
36481513	5	47	theme	shell	940:944	arg1	polymer					946:952	shell polymer	940:952	shell polymer	940:952	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	2	48	theme	acid	347:350	arg1	encapsulates					379:390	iron, ascorbic acid, and folic acid core-shell encapsulates	332:390	iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers	332:538	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	4	49	theme	lightness	807:815	arg1	value					817:821	the lightness value	803:821	the lightness value (52.91 ± 0.13)	803:836	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	4	49	theme	lightness	807:815	arg1	52.91 ± 0.13					824:835	52.91 ± 0.13	824:835	52.91 ± 0.13	824:835	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	8	50	theme	whey	1555:1558	arg1	pH					1568:1569	whey protein pH	1555:1569	whey protein pH	1555:1569	The encapsulates prepared without adjusting whey protein pH showed the least release (∼51 % in 24 h) and bioaccessibility (∼56%) of iron indicating the iron-whey protein complex formation.					
36481513	6	51	theme	highest	1190:1196	arg1	60.80 ± 0.32					1215:1226	60.80 ± 0.32	1215:1226	60.80 ± 0.32	1215:1226	Whereas, encapsulates having HPMC as shell polymer showed highest lightness value (60.80 ± 0.32) and highest encapsulation efficiency for iron (87.28 ± 4.15%).					
36481513	6	51	theme	highest	1190:1196	arg1	value					1208:1212	highest lightness value	1190:1212	highest lightness value (60.80 ± 0.32)	1190:1227	Whereas, encapsulates having HPMC as shell polymer showed highest lightness value (60.80 ± 0.32) and highest encapsulation efficiency for iron (87.28 ± 4.15%).					
36481513	5	52	with	efficiency	1089:1098	arg1	values					993:998	lowest values	986:998	lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71)	986:1061	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	7	53	theme	composition	1373:1383	arg1	evaluation					1347:1356	evaluation	1347:1356	evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying	1347:1508	The formation of core-shell structure was confirmed by evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying.					
36481513	9	54	theme	protein	1805:1811	arg1	combination					1785:1795	a combination	1783:1795	a combination of whey protein and pectin	1783:1822	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	1	55	theme	nutrients	177:185	arg1	encapsulation					154:166	encapsulation	154:166	encapsulation of these nutrients and fortification into foods	154:214	Considering the growing concern of iron and folic acid deficiency, encapsulation of these nutrients and fortification into foods is emerging as an effective counter-strategy.					
36481513	9	56	theme	pectin	1817:1822	arg1	combination					1785:1795	a combination	1783:1795	a combination of whey protein and pectin	1783:1822	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	1	57	theme	fortification	191:203	arg1	encapsulation					154:166	encapsulation	154:166	encapsulation of these nutrients and fortification into foods	154:214	Considering the growing concern of iron and folic acid deficiency, encapsulation of these nutrients and fortification into foods is emerging as an effective counter-strategy.					
36481513	7	58	theme	core-shell	1309:1318	arg1	structure					1320:1328	core-shell structure	1309:1328	core-shell structure	1309:1328	The formation of core-shell structure was confirmed by evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying.					
36481513	6	59	theme	encapsulation	1241:1253	arg1	%					1288:1288	87.28 ± 4.15%	1276:1288	87.28 ± 4.15%	1276:1288	Whereas, encapsulates having HPMC as shell polymer showed highest lightness value (60.80 ± 0.32) and highest encapsulation efficiency for iron (87.28 ± 4.15%).					
36481513	6	59	theme	encapsulation	1241:1253	arg1	efficiency					1255:1264	highest encapsulation efficiency	1233:1264	highest encapsulation efficiency for iron (87.28 ± 4.15%)	1233:1289	Whereas, encapsulates having HPMC as shell polymer showed highest lightness value (60.80 ± 0.32) and highest encapsulation efficiency for iron (87.28 ± 4.15%).					
36481513	5	60	theme	ratio	1011:1015	arg1	values					993:998	lowest values	986:998	lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71)	986:1061	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	3	61	theme	shell	555:559	arg1	formation					561:569	shell formation	555:569	shell formation	555:569	The effect of shell formation was observed by comparing core-shell encapsulates with conventional 2-fluid nozzle (2FN) encapsulates.					
36481513	8	62	theme	iron	1643:1646	arg1	release					1588:1594	the least release	1578:1594	the least release (∼51 % in 24 h)	1578:1610	The encapsulates prepared without adjusting whey protein pH showed the least release (∼51 % in 24 h) and bioaccessibility (∼56%) of iron indicating the iron-whey protein complex formation.					
36481513	8	62	theme	iron	1643:1646	arg1	bioaccessibility					1616:1631	bioaccessibility	1616:1631	bioaccessibility (∼56%) of iron	1616:1646	The encapsulates prepared without adjusting whey protein pH showed the least release (∼51 % in 24 h) and bioaccessibility (∼56%) of iron indicating the iron-whey protein complex formation.					
36481513	8	62	theme	iron	1643:1646	arg1	%					1637:1637	∼56%	1634:1637	∼56%	1634:1637	The encapsulates prepared without adjusting whey protein pH showed the least release (∼51 % in 24 h) and bioaccessibility (∼56%) of iron indicating the iron-whey protein complex formation.					
36481513	9	63	theme	iron	1863:1866	arg1	acid					1875:1878	folic acid	1869:1878	folic acid	1869:1878	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	9	63	theme	iron	1863:1866	arg1	co-encapsulation					1843:1858	co-encapsulation	1843:1858	co-encapsulation of iron	1843:1866	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	9	63	theme	iron	1863:1866	arg1	acid					1893:1896	ascorbic acid	1884:1896	ascorbic acid	1884:1896	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	5	64	theme	lowest	986:991	arg1	values					993:998	lowest values	986:998	lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71)	986:1061	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	2	65	theme	present	266:272	arg1	work					274:277	The present work	262:277	The present work	262:277	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	2	66	theme	shell	525:529	arg1	polymers					531:538	shell polymers	525:538	shell polymers	525:538	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	2	66	theme	shell	525:529	arg1	drying					431:436	novel 3-fluid nozzle (3FN) spray drying	398:436	novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC)	398:520	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	9	67	theme	folic	1869:1873	arg1	acid					1875:1878	folic acid	1869:1878	folic acid	1869:1878	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	9	67	theme	folic	1869:1873	arg1	co-encapsulation					1843:1858	co-encapsulation	1843:1858	co-encapsulation of iron	1843:1866	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	0	68	theme	spray	73:77	arg1	drying					79:84	spray drying	73:84	spray drying	73:84	Development of iron-vitamin multilayer encapsulates using 3 fluid nozzle spray drying.					
36481513	2	69	with	drying	431:436	arg1	protein					448:454	whey protein	443:454	whey protein	443:454	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	1	70	theme	acid	137:140	arg1	deficiency					142:151	folic acid deficiency	131:151	folic acid deficiency	131:151	Considering the growing concern of iron and folic acid deficiency, encapsulation of these nutrients and fortification into foods is emerging as an effective counter-strategy.					
36481513	7	71	theme	spray	1497:1501	arg1	drying					1503:1508	3FN spray drying	1493:1508	3FN spray drying	1493:1508	The formation of core-shell structure was confirmed by evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying.					
36481513	2	72	theme	hydroxypropyl	485:497	arg1	methylcellulose					499:513	hydroxypropyl methylcellulose	485:513	hydroxypropyl methylcellulose	485:513	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	2	73	theme	core-shell	368:377	arg1	encapsulates					379:390	iron, ascorbic acid, and folic acid core-shell encapsulates	332:390	iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers	332:538	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	4	74	theme	pH	694:695	arg1	noteworthy					745:754	noteworthy	745:754	noteworthy	745:754	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	4	74	theme	pH	694:695	arg1	effect					684:689	the effect	680:689	the effect of pH of whey protein on the color of encapsulates	680:740	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	2	75	theme	folic	357:361	arg1	acid					363:366	folic acid	357:366	folic acid	357:366	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	0	76	theme	multilayer	28:37	arg1	encapsulates					39:50	iron-vitamin multilayer encapsulates	15:50	iron-vitamin multilayer encapsulates using 3 fluid	15:64	Development of iron-vitamin multilayer encapsulates using 3 fluid nozzle spray drying.					
36481513	5	77	theme	folic	1104:1108	arg1	acid					1110:1113	folic acid	1104:1113	folic acid (86.07 ± 5.24%)	1104:1129	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	5	77	theme	folic	1104:1108	arg1	%					1128:1128	86.07 ± 5.24%	1116:1128	86.07 ± 5.24%	1116:1128	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	1	78	theme	growing	103:109	arg1	concern					111:117	the growing concern	99:117	the growing concern of iron and folic acid deficiency	99:151	Considering the growing concern of iron and folic acid deficiency, encapsulation of these nutrients and fortification into foods is emerging as an effective counter-strategy.					
36481513	4	79	theme	whey	700:703	arg1	protein					705:711	whey protein	700:711	whey protein	700:711	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	2	80	theme	3-fluid	404:410	arg1	polymers					531:538	shell polymers	525:538	shell polymers	525:538	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	2	80	theme	3-fluid	404:410	arg1	drying					431:436	novel 3-fluid nozzle (3FN) spray drying	398:436	novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC)	398:520	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	2	81	theme	whey	443:446	arg1	protein					448:454	whey protein	443:454	whey protein	443:454	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	3	82	theme	core-shell	597:606	arg1	encapsulates					608:619	core-shell encapsulates	597:619	core-shell encapsulates	597:619	The effect of shell formation was observed by comparing core-shell encapsulates with conventional 2-fluid nozzle (2FN) encapsulates.					
36481513	4	83	theme	reducing	757:764	arg1	pH					770:771	the pH	766:771	reducing the pH to 4.0	757:778	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	5	84	theme	highest	1067:1073	arg1	efficiency					1089:1098	highest encapsulation efficiency	1067:1098	highest encapsulation efficiency for folic acid (86.07 ± 5.24%)	1067:1129	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	5	85	theme	fair	964:967	arg1	flowability					969:979	fair flowability	964:979	fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71)	964:1061	Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%).					
36481513	2	86	theme	iron	332:335	arg1	encapsulates					379:390	iron, ascorbic acid, and folic acid core-shell encapsulates	332:390	iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers	332:538	The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers.					
36481513	8	87	theme	least	1582:1586	arg1	release					1588:1594	the least release	1578:1594	the least release (∼51 % in 24 h)	1578:1610	The encapsulates prepared without adjusting whey protein pH showed the least release (∼51 % in 24 h) and bioaccessibility (∼56%) of iron indicating the iron-whey protein complex formation.					
36481513	3	88	theme	2-fluid	639:645	arg1	encapsulates					660:671	conventional 2-fluid nozzle (2FN) encapsulates	626:671	conventional 2-fluid nozzle (2FN) encapsulates	626:671	The effect of shell formation was observed by comparing core-shell encapsulates with conventional 2-fluid nozzle (2FN) encapsulates.					
36481513	4	89	theme	encapsulates	729:740	arg1	color					720:724	the color	716:724	the color of encapsulates	716:740	Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58).					
36481513	7	90	theme	increased	1422:1430	arg1	bonds					1455:1459	increased aliphatic and carbonyl bonds	1422:1459	increased aliphatic and carbonyl bonds	1422:1459	The formation of core-shell structure was confirmed by evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying.					
36481513	9	91	theme	release	1765:1771	arg1	behavior					1773:1780	release behavior	1765:1780	release behavior	1765:1780	Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.					
36481513	1	92	theme	effective	234:242	arg1	counter-strategy					244:259	an effective counter-strategy	231:259	an effective counter-strategy	231:259	Considering the growing concern of iron and folic acid deficiency, encapsulation of these nutrients and fortification into foods is emerging as an effective counter-strategy.					
36343837	0	0	theme	coated	91:96	arg1	Cellulose					143:151	coated cellulose/polyurethane nanofibrous membrane: Cellulose	91:151	coated cellulose/polyurethane nanofibrous membrane: Cellulose	91:151	High performance flexible and antibacterial strain sensor based on silver‑carbon nanotubes coated cellulose/polyurethane nanofibrous membrane: Cellulose as reinforcing polymer blend and polydopamine as compatibilizer.					
36343837	7	1	theme	rigid	1331:1335	arg1	polysaccharide					1337:1350	rigid polysaccharide	1331:1350	rigid polysaccharide	1331:1350	This study proposed a novel thinking for the construction of high performance strain sensor by rational introduction of rigid polysaccharide into the polymer matrix.					
36343837	4	2	theme	parts	967:971	arg1	motion					946:951	real-time motion	936:951	real-time motion of human body parts	936:971	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	7	3	theme	polymer	1361:1367	arg1	matrix					1369:1374	the polymer matrix	1357:1374	the polymer matrix	1357:1374	This study proposed a novel thinking for the construction of high performance strain sensor by rational introduction of rigid polysaccharide into the polymer matrix.					
36343837	1	4	theme	second-phase	265:276	arg1	polymer					278:284	the second-phase polymer	261:284	the second-phase polymer blended with polyurethane	261:310	In this study, ethyl cellulose was used as the second-phase polymer blended with polyurethane to make nanofibrous membrane as antibacterial strain sensor.					
36343837	1	4	theme	second-phase	265:276	arg1	cellulose					239:247	ethyl cellulose	233:247	ethyl cellulose	233:247	In this study, ethyl cellulose was used as the second-phase polymer blended with polyurethane to make nanofibrous membrane as antibacterial strain sensor.					
36343837	7	5	theme	polysaccharide	1337:1350	arg1	introduction					1315:1326	rational introduction	1306:1326	rational introduction of rigid polysaccharide into the polymer matrix	1306:1374	This study proposed a novel thinking for the construction of high performance strain sensor by rational introduction of rigid polysaccharide into the polymer matrix.					
36343837	4	6	theme	body	962:965	arg1	parts					967:971	human body parts	956:971	human body parts	956:971	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	6	7	theme	membrane	1201:1208	arg1	stability					1184:1192	the shape recovery and longtime use stability	1148:1192	stability	1184:1192	The cellulose in the nanofiber structure ensured the shape recovery and longtime use stability of the membrane.					
36343837	6	7	theme	membrane	1201:1208	arg1	recovery					1158:1165	the shape recovery and longtime use stability	1148:1192	recovery	1158:1165	The cellulose in the nanofiber structure ensured the shape recovery and longtime use stability of the membrane.					
36343837	7	8	theme	novel	1233:1237	arg1	thinking					1239:1246	a novel thinking	1231:1246	a novel thinking for the construction of high performance strain sensor	1231:1301	This study proposed a novel thinking for the construction of high performance strain sensor by rational introduction of rigid polysaccharide into the polymer matrix.					
36343837	0	9	theme	nanofibrous	121:131	arg1	Cellulose					143:151	coated cellulose/polyurethane nanofibrous membrane: Cellulose	91:151	coated cellulose/polyurethane nanofibrous membrane: Cellulose	91:151	High performance flexible and antibacterial strain sensor based on silver‑carbon nanotubes coated cellulose/polyurethane nanofibrous membrane: Cellulose as reinforcing polymer blend and polydopamine as compatibilizer.					
36343837	5	10	theme	varied	1077:1082	arg1	motions					1090:1096	varied human motions	1077:1096	varied human motions	1077:1096	The conductive composite membrane possessed sensitive and regular resistance feedback to 100 cycles of varied human motions.					
36343837	0	11	theme	cellulose/polyurethane	98:119	arg1	Cellulose					143:151	coated cellulose/polyurethane nanofibrous membrane: Cellulose	91:151	coated cellulose/polyurethane nanofibrous membrane: Cellulose	91:151	High performance flexible and antibacterial strain sensor based on silver‑carbon nanotubes coated cellulose/polyurethane nanofibrous membrane: Cellulose as reinforcing polymer blend and polydopamine as compatibilizer.					
36343837	5	12	theme	human	1084:1088	arg1	motions					1090:1096	varied human motions	1077:1096	varied human motions	1077:1096	The conductive composite membrane possessed sensitive and regular resistance feedback to 100 cycles of varied human motions.					
36343837	5	13	theme	resistance	1040:1049	arg1	feedback					1051:1058	sensitive and regular resistance feedback	1018:1058	sensitive and regular resistance feedback	1018:1058	The conductive composite membrane possessed sensitive and regular resistance feedback to 100 cycles of varied human motions.					
36343837	6	14	from	cellulose	1103:1111	arg1	structure					1130:1138	the nanofiber structure	1116:1138	the nanofiber structure	1116:1138	The cellulose in the nanofiber structure ensured the shape recovery and longtime use stability of the membrane.					
36343837	5	15	theme	motions	1090:1096	arg1	100 cycles					1063:1072	100 cycles	1063:1072	100 cycles of varied human motions	1063:1096	The conductive composite membrane possessed sensitive and regular resistance feedback to 100 cycles of varied human motions.					
36343837	4	16	theme	carbon	741:746	arg1	nanotubes					748:756	carbon nanotubes	741:756	carbon nanotubes	741:756	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	0	17	theme	membrane	133:140	arg1	Cellulose					143:151	coated cellulose/polyurethane nanofibrous membrane: Cellulose	91:151	coated cellulose/polyurethane nanofibrous membrane: Cellulose	91:151	High performance flexible and antibacterial strain sensor based on silver‑carbon nanotubes coated cellulose/polyurethane nanofibrous membrane: Cellulose as reinforcing polymer blend and polydopamine as compatibilizer.					
36343837	2	18	theme	nanofiber	525:533	arg1	uniformity					535:544	the nanofiber uniformity	521:544	the nanofiber uniformity of polyurethane	521:560	The results indicated that ethyl cellulose could regulate the morphology of polyurethane through strong hydrogen bonding, which observably enhanced the nanofiber uniformity of polyurethane.					
36343837	4	19	theme	outstanding	862:872	arg1	sensitivity					874:884	outstanding sensitivity	862:884	outstanding sensitivity toward external mechanical stretching	862:922	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	7	20	theme	strain	1289:1294	arg1	sensor					1296:1301	high performance strain sensor	1272:1301	high performance strain sensor	1272:1301	This study proposed a novel thinking for the construction of high performance strain sensor by rational introduction of rigid polysaccharide into the polymer matrix.					
36343837	4	21	theme	human	956:960	arg1	parts					967:971	human body parts	956:971	human body parts	956:971	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	4	22	theme	outstanding	808:818	arg1	bacteria					820:827	outstanding bacteria	808:827	outstanding bacteria inhibition performance	808:850	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	4	23	theme	assisted	758:765	arg1	nanoparticles					723:735	silver nanoparticles	716:735	silver nanoparticles	716:735	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	7	24	theme	sensor	1296:1301	arg1	construction					1256:1267	the construction	1252:1267	the construction of high performance strain sensor	1252:1301	This study proposed a novel thinking for the construction of high performance strain sensor by rational introduction of rigid polysaccharide into the polymer matrix.					
36343837	3	25	theme	thermal	645:651	arg1	stability					653:661	thermal stability	645:661	thermal stability	645:661	Furthermore, rigid cellulose also remarkably improved the mechanical strength and thermal stability of the nanofibrous membrane.					
36343837	6	26	theme	use	1180:1182	arg1	stability					1184:1192	the shape recovery and longtime use stability	1148:1192	stability	1184:1192	The cellulose in the nanofiber structure ensured the shape recovery and longtime use stability of the membrane.					
36343837	7	27	theme	high	1272:1275	arg1	sensor					1296:1301	high performance strain sensor	1272:1301	high performance strain sensor	1272:1301	This study proposed a novel thinking for the construction of high performance strain sensor by rational introduction of rigid polysaccharide into the polymer matrix.					
36343837	3	28	theme	mechanical	621:630	arg1	strength					632:639	mechanical strength	621:639	mechanical strength	621:639	Furthermore, rigid cellulose also remarkably improved the mechanical strength and thermal stability of the nanofibrous membrane.					
36343837	2	29	theme	strong	470:475	arg1	bonding					486:492	strong hydrogen bonding	470:492	strong hydrogen bonding	470:492	The results indicated that ethyl cellulose could regulate the morphology of polyurethane through strong hydrogen bonding, which observably enhanced the nanofiber uniformity of polyurethane.					
36343837	2	30	theme	hydrogen	477:484	arg1	bonding					486:492	strong hydrogen bonding	470:492	strong hydrogen bonding	470:492	The results indicated that ethyl cellulose could regulate the morphology of polyurethane through strong hydrogen bonding, which observably enhanced the nanofiber uniformity of polyurethane.					
36343837	4	31	theme	bacteria	820:827	arg1	performance					840:850	outstanding bacteria inhibition performance	808:850	outstanding bacteria inhibition performance	808:850	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	5	32	theme	conductive	978:987	arg1	membrane					999:1006	The conductive composite membrane	974:1006	The conductive composite membrane	974:1006	The conductive composite membrane possessed sensitive and regular resistance feedback to 100 cycles of varied human motions.					
36343837	0	33	theme	strain	44:49	arg1	sensor					51:56	High performance flexible and antibacterial strain sensor	0:56	sensor	51:56	High performance flexible and antibacterial strain sensor based on silver‑carbon nanotubes coated cellulose/polyurethane nanofibrous membrane: Cellulose as reinforcing polymer blend and polydopamine as compatibilizer.					
36343837	1	34	theme	nanofibrous	320:330	arg1	membrane					332:339	nanofibrous membrane	320:339	nanofibrous membrane	320:339	In this study, ethyl cellulose was used as the second-phase polymer blended with polyurethane to make nanofibrous membrane as antibacterial strain sensor.					
36343837	0	35	theme	polymer	168:174	arg1	blend					176:180	polymer blend	168:180	polymer blend	168:180	High performance flexible and antibacterial strain sensor based on silver‑carbon nanotubes coated cellulose/polyurethane nanofibrous membrane: Cellulose as reinforcing polymer blend and polydopamine as compatibilizer.					
36343837	1	36	theme	ethyl	233:237	arg1	polymer					278:284	the second-phase polymer	261:284	the second-phase polymer blended with polyurethane	261:310	In this study, ethyl cellulose was used as the second-phase polymer blended with polyurethane to make nanofibrous membrane as antibacterial strain sensor.					
36343837	1	36	theme	ethyl	233:237	arg1	cellulose					239:247	ethyl cellulose	233:247	ethyl cellulose	233:247	In this study, ethyl cellulose was used as the second-phase polymer blended with polyurethane to make nanofibrous membrane as antibacterial strain sensor.					
36343837	2	37	theme	polyurethane	449:460	arg1	morphology					435:444	the morphology	431:444	the morphology of polyurethane	431:460	The results indicated that ethyl cellulose could regulate the morphology of polyurethane through strong hydrogen bonding, which observably enhanced the nanofiber uniformity of polyurethane.					
36343837	0	38	theme	antibacterial	30:42	arg1	sensor					51:56	High performance flexible and antibacterial strain sensor	0:56	sensor	51:56	High performance flexible and antibacterial strain sensor based on silver‑carbon nanotubes coated cellulose/polyurethane nanofibrous membrane: Cellulose as reinforcing polymer blend and polydopamine as compatibilizer.					
36343837	3	39	theme	nanofibrous	670:680	arg1	membrane					682:689	the nanofibrous membrane	666:689	the nanofibrous membrane	666:689	Furthermore, rigid cellulose also remarkably improved the mechanical strength and thermal stability of the nanofibrous membrane.					
36343837	4	40	theme	silver	716:721	arg1	nanoparticles					723:735	silver nanoparticles	716:735	silver nanoparticles	716:735	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	6	41	theme	shape	1152:1156	arg1	recovery					1158:1165	the shape recovery and longtime use stability	1148:1192	recovery	1158:1165	The cellulose in the nanofiber structure ensured the shape recovery and longtime use stability of the membrane.					
36343837	5	42	theme	sensitive	1018:1026	arg1	feedback					1051:1058	sensitive and regular resistance feedback	1018:1058	sensitive and regular resistance feedback	1018:1058	The conductive composite membrane possessed sensitive and regular resistance feedback to 100 cycles of varied human motions.					
36343837	1	43	theme	antibacterial	344:356	arg1	sensor					365:370	antibacterial strain sensor	344:370	antibacterial strain sensor	344:370	In this study, ethyl cellulose was used as the second-phase polymer blended with polyurethane to make nanofibrous membrane as antibacterial strain sensor.					
36343837	7	44	theme	rational	1306:1313	arg1	introduction					1315:1326	rational introduction	1306:1326	rational introduction of rigid polysaccharide into the polymer matrix	1306:1374	This study proposed a novel thinking for the construction of high performance strain sensor by rational introduction of rigid polysaccharide into the polymer matrix.					
36343837	4	45	theme	real-time	936:944	arg1	motion					946:951	real-time motion	936:951	real-time motion of human body parts	936:971	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	7	46	theme	performance	1277:1287	arg1	sensor					1296:1301	high performance strain sensor	1272:1301	high performance strain sensor	1272:1301	This study proposed a novel thinking for the construction of high performance strain sensor by rational introduction of rigid polysaccharide into the polymer matrix.					
36343837	1	47	theme	strain	358:363	arg1	sensor					365:370	antibacterial strain sensor	344:370	antibacterial strain sensor	344:370	In this study, ethyl cellulose was used as the second-phase polymer blended with polyurethane to make nanofibrous membrane as antibacterial strain sensor.					
36343837	4	48	theme	external	893:900	arg1	stretching					913:922	external mechanical stretching	893:922	external mechanical stretching	893:922	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	5	49	theme	regular	1032:1038	arg1	feedback					1051:1058	sensitive and regular resistance feedback	1018:1058	sensitive and regular resistance feedback	1018:1058	The conductive composite membrane possessed sensitive and regular resistance feedback to 100 cycles of varied human motions.					
36343837	4	50	theme	inhibition	829:838	arg1	performance					840:850	outstanding bacteria inhibition performance	808:850	outstanding bacteria inhibition performance	808:850	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	1	51	used	used	253:256	arg2	cellulose					239:247	ethyl cellulose	233:247	ethyl cellulose	233:247	In this study, ethyl cellulose was used as the second-phase polymer blended with polyurethane to make nanofibrous membrane as antibacterial strain sensor.					
36343837	1	51	used	used	253:256	arg2	polymer					278:284	the second-phase polymer	261:284	the second-phase polymer blended with polyurethane	261:310	In this study, ethyl cellulose was used as the second-phase polymer blended with polyurethane to make nanofibrous membrane as antibacterial strain sensor.					
36343837	5	52	contain	possessed	1008:1016	arg1	membrane					999:1006	The conductive composite membrane	974:1006	The conductive composite membrane	974:1006	The conductive composite membrane possessed sensitive and regular resistance feedback to 100 cycles of varied human motions.					
36343837	5	52	contain	possessed	1008:1016	arg2	feedback					1051:1058	sensitive and regular resistance feedback	1018:1058	sensitive and regular resistance feedback	1018:1058	The conductive composite membrane possessed sensitive and regular resistance feedback to 100 cycles of varied human motions.					
36343837	3	53	dep	strength	632:639	arg1	the					617:619	the	617:619	the	617:619	Furthermore, rigid cellulose also remarkably improved the mechanical strength and thermal stability of the nanofibrous membrane.					
36343837	5	54	theme	composite	989:997	arg1	membrane					999:1006	The conductive composite membrane	974:1006	The conductive composite membrane	974:1006	The conductive composite membrane possessed sensitive and regular resistance feedback to 100 cycles of varied human motions.					
36343837	3	55	theme	membrane	682:689	arg1	strength					632:639	mechanical strength	621:639	mechanical strength	621:639	Furthermore, rigid cellulose also remarkably improved the mechanical strength and thermal stability of the nanofibrous membrane.					
36343837	3	55	theme	membrane	682:689	arg1	stability					653:661	thermal stability	645:661	thermal stability	645:661	Furthermore, rigid cellulose also remarkably improved the mechanical strength and thermal stability of the nanofibrous membrane.					
36343837	6	56	theme	longtime	1171:1178	arg1	stability					1184:1192	the shape recovery and longtime use stability	1148:1192	stability	1184:1192	The cellulose in the nanofiber structure ensured the shape recovery and longtime use stability of the membrane.					
36343837	3	57	theme	rigid	576:580	arg1	cellulose					582:590	rigid cellulose	576:590	rigid cellulose	576:590	Furthermore, rigid cellulose also remarkably improved the mechanical strength and thermal stability of the nanofibrous membrane.					
36343837	2	58	theme	polyurethane	549:560	arg1	uniformity					535:544	the nanofiber uniformity	521:544	the nanofiber uniformity of polyurethane	521:560	The results indicated that ethyl cellulose could regulate the morphology of polyurethane through strong hydrogen bonding, which observably enhanced the nanofiber uniformity of polyurethane.					
36343837	4	59	with	membrane	794:801	arg1	performance					840:850	outstanding bacteria inhibition performance	808:850	outstanding bacteria inhibition performance	808:850	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	4	60	theme	mechanical	902:911	arg1	stretching					913:922	external mechanical stretching	893:922	external mechanical stretching	893:922	After being coated with silver nanoparticles and carbon nanotubes assisted by polydopamine (PDA), the membrane with outstanding bacteria inhibition performance exhibited outstanding sensitivity toward external mechanical stretching, as well as real-time motion of human body parts.					
36343837	2	61	theme	ethyl	400:404	arg1	cellulose					406:414	ethyl cellulose	400:414	ethyl cellulose	400:414	The results indicated that ethyl cellulose could regulate the morphology of polyurethane through strong hydrogen bonding, which observably enhanced the nanofiber uniformity of polyurethane.					
36343837	6	62	theme	nanofiber	1120:1128	arg1	structure					1130:1138	the nanofiber structure	1116:1138	the nanofiber structure	1116:1138	The cellulose in the nanofiber structure ensured the shape recovery and longtime use stability of the membrane.					
37076070	8	0	dep	in	1434:1435	arg1	vivo					1437:1440	vivo	1437:1440	vivo	1437:1440	LB1932 enriched hydrogel was found to be of benefit compared to control dressings, giving the more promising results as potential for in vivo skin wound healing tests.					
37076070	7	1	theme	spectrometry	1111:1122	arg1	analysis					1151:1158	liquid chromatography-mass spectrometry targeted protein biomarker analysis	1084:1158	liquid chromatography-mass spectrometry targeted protein biomarker analysis	1084:1158	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	7	2	theme	targeted	1124:1131	arg1	analysis					1151:1158	liquid chromatography-mass spectrometry targeted protein biomarker analysis	1084:1158	liquid chromatography-mass spectrometry targeted protein biomarker analysis	1084:1158	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	8	3	theme	enriched	1307:1314	arg1	hydrogel					1316:1323	LB1932 enriched hydrogel	1300:1323	LB1932 enriched hydrogel	1300:1323	LB1932 enriched hydrogel was found to be of benefit compared to control dressings, giving the more promising results as potential for in vivo skin wound healing tests.					
37076070	7	4	from	increase	1240:1247	arg1	production					1288:1297	collagen and antiapoptotic proteins production	1252:1297	collagen and antiapoptotic proteins production	1252:1297	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	3	5	used	used	333:336	arg2	biomaterials					464:475	high-value functional biomaterials	442:475	high-value functional biomaterials with therapeutic potentials in regenerative medicine applications	442:541	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	3	5	used	used	333:336	arg2	LB					275:276	LB	275:276	LB	275:276	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	3	5	used	used	333:336	arg2	bulgaricus					263:272	bulgaricus	263:272	bulgaricus (LB)	263:277	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	6	6	theme	cell	896:899	arg1	proliferation					901:913	cell proliferation	896:913	cell proliferation	896:913	The derivatives showed an ability to increase cell proliferation and migration, quantifiable between 10 and 20 % if compared to controls, with higher values for the derivatives obtained from the LB1932 strain.					
37076070	8	7	theme	potential	1420:1428	arg1	tests					1461:1465	potential for in vivo skin wound healing tests	1420:1465	potential for in vivo skin wound healing tests	1420:1465	LB1932 enriched hydrogel was found to be of benefit compared to control dressings, giving the more promising results as potential for in vivo skin wound healing tests.					
37076070	8	7	theme	potential	1420:1428	arg1	results					1409:1415	the more promising results	1390:1415	the more promising results	1390:1415	LB1932 enriched hydrogel was found to be of benefit compared to control dressings, giving the more promising results as potential for in vivo skin wound healing tests.					
37076070	3	8	theme	self-crosslinking	365:381	arg1	hydrogels					428:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	7	9	theme	proapoptotic	1198:1209	arg1	proteins					1211:1218	matrix-degrading and proapoptotic proteins	1177:1218	matrix-degrading and proapoptotic proteins	1177:1218	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	1	10	theme	printed	63:69	arg1	hydrogels					114:122	3D printed alginate/hyaluronic acid self-crosslinking hydrogels	60:122	3D printed alginate/hyaluronic acid self-crosslinking hydrogels	60:122	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	3	11	theme	3D	383:384	arg1	hydrogels					428:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	8	12	theme	in	1434:1435	arg1	healing					1453:1459	in vivo skin wound healing	1434:1459	in vivo skin wound healing	1434:1459	LB1932 enriched hydrogel was found to be of benefit compared to control dressings, giving the more promising results as potential for in vivo skin wound healing tests.					
37076070	1	13	theme	alginate/hyaluronic	71:89	arg1	hydrogels					114:122	3D printed alginate/hyaluronic acid self-crosslinking hydrogels	60:122	3D printed alginate/hyaluronic acid self-crosslinking hydrogels	60:122	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	6	14	theme	LB1932	1045:1050	arg1	strain					1052:1057	the LB1932 strain	1041:1057	the LB1932 strain	1041:1057	The derivatives showed an ability to increase cell proliferation and migration, quantifiable between 10 and 20 % if compared to controls, with higher values for the derivatives obtained from the LB1932 strain.					
37076070	7	15	theme	matrix-degrading	1177:1192	arg1	proteins					1211:1218	matrix-degrading and proapoptotic proteins	1177:1218	matrix-degrading and proapoptotic proteins	1177:1218	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	1	16	theme	acid	91:94	arg1	hydrogels					114:122	3D printed alginate/hyaluronic acid self-crosslinking hydrogels	60:122	3D printed alginate/hyaluronic acid self-crosslinking hydrogels	60:122	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	2	17	from	subsp	256:260	arg1	EPS					219:221	EPS	219:221	EPS	219:221	Derivatives [i.e. proteins and exopolysaccharides (EPS)] from Lactobacillus delbrueckii subsp.					
37076070	2	17	from	subsp	256:260	arg1	exopolysaccharides					199:216	exopolysaccharides	199:216	exopolysaccharides	199:216	Derivatives [i.e. proteins and exopolysaccharides (EPS)] from Lactobacillus delbrueckii subsp.					
37076070	2	17	from	subsp	256:260	arg1	proteins					186:193	proteins	186:193	proteins	186:193	Derivatives [i.e. proteins and exopolysaccharides (EPS)] from Lactobacillus delbrueckii subsp.					
37076070	3	18	theme	functional	453:462	arg1	bulgaricus					263:272	bulgaricus	263:272	bulgaricus (LB)	263:277	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	3	18	theme	functional	453:462	arg1	biomaterials					464:475	high-value functional biomaterials	442:475	high-value functional biomaterials with therapeutic potentials in regenerative medicine applications	442:541	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	3	19	theme	novel	359:363	arg1	hydrogels					428:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	8	20	theme	skin	1442:1445	arg1	healing					1453:1459	in vivo skin wound healing	1434:1459	in vivo skin wound healing	1434:1459	LB1932 enriched hydrogel was found to be of benefit compared to control dressings, giving the more promising results as potential for in vivo skin wound healing tests.					
37076070	1	21	theme	self-crosslinking	96:112	arg1	hydrogels					114:122	3D printed alginate/hyaluronic acid self-crosslinking hydrogels	60:122	3D printed alginate/hyaluronic acid self-crosslinking hydrogels	60:122	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	3	22	theme	acid	414:417	arg1	hydrogels					428:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	4	23	theme	human	715:719	arg1	fibroblast					721:730	human fibroblast	715:730	human fibroblast	715:730	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	7	24	theme	proteins	1279:1286	arg1	production					1288:1297	collagen and antiapoptotic proteins production	1252:1297	collagen and antiapoptotic proteins production	1252:1297	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	7	25	from	decrease	1165:1172	arg1	proteins					1211:1218	matrix-degrading and proapoptotic proteins	1177:1218	matrix-degrading and proapoptotic proteins	1177:1218	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	5	26	theme	relevant	778:785	arg1	cytocompatibility					802:818	relevant dose-dependent cytocompatibility	778:818	relevant dose-dependent cytocompatibility	778:818	EPS received particular attention as showing relevant dose-dependent cytocompatibility against the human fibroblast.					
37076070	3	27	theme	high-value	442:451	arg1	bulgaricus					263:272	bulgaricus	263:272	bulgaricus (LB)	263:277	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	3	27	theme	high-value	442:451	arg1	biomaterials					464:475	high-value functional biomaterials	442:475	high-value functional biomaterials with therapeutic potentials in regenerative medicine applications	442:541	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	3	28	theme	printed	386:392	arg1	hydrogels					428:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	8	29	theme	promising	1399:1407	arg1	tests					1461:1465	potential for in vivo skin wound healing tests	1420:1465	potential for in vivo skin wound healing tests	1420:1465	LB1932 enriched hydrogel was found to be of benefit compared to control dressings, giving the more promising results as potential for in vivo skin wound healing tests.					
37076070	8	29	theme	promising	1399:1407	arg1	results					1409:1415	the more promising results	1390:1415	the more promising results	1390:1415	LB1932 enriched hydrogel was found to be of benefit compared to control dressings, giving the more promising results as potential for in vivo skin wound healing tests.					
37076070	5	30	theme	particular	746:755	arg1	attention					757:765	particular attention	746:765	particular attention	746:765	EPS received particular attention as showing relevant dose-dependent cytocompatibility against the human fibroblast.					
37076070	8	31	theme	control	1364:1370	arg1	dressings					1372:1380	control dressings	1364:1380	control dressings	1364:1380	LB1932 enriched hydrogel was found to be of benefit compared to control dressings, giving the more promising results as potential for in vivo skin wound healing tests.					
37076070	3	32	theme	alginate/hyaluronic	394:412	arg1	hydrogels					428:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	8	33	theme	wound	1447:1451	arg1	healing					1453:1459	in vivo skin wound healing	1434:1459	in vivo skin wound healing	1434:1459	LB1932 enriched hydrogel was found to be of benefit compared to control dressings, giving the more promising results as potential for in vivo skin wound healing tests.					
37076070	4	34	from	effect	674:679	arg1	migration					702:710	migration	702:710	migration	702:710	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	4	34	from	effect	674:679	arg1	fibroblast					721:730	human fibroblast	715:730	human fibroblast	715:730	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	4	34	from	effect	674:679	arg1	proliferation					684:696	proliferation	684:696	proliferation	684:696	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	3	35	theme	hydrogels	428:436	arg1	production					345:354	the production	341:354	the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	341:436	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	7	36	theme	biomarker	1141:1149	arg1	analysis					1151:1158	liquid chromatography-mass spectrometry targeted protein biomarker analysis	1084:1158	liquid chromatography-mass spectrometry targeted protein biomarker analysis	1084:1158	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	8	37	theme	LB1932	1300:1305	arg1	hydrogel					1316:1323	LB1932 enriched hydrogel	1300:1323	LB1932 enriched hydrogel	1300:1323	LB1932 enriched hydrogel was found to be of benefit compared to control dressings, giving the more promising results as potential for in vivo skin wound healing tests.					
37076070	4	38	theme	LB	582:583	arg1	strains					585:591	two different LB strains	568:591	two different LB strains	568:591	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	4	38	theme	LB	582:583	arg1	LB1932					605:610	LB1932	605:610	LB1932	605:610	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	4	38	theme	LB	582:583	arg1	LB1865					594:599	LB1865	594:599	LB1865	594:599	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	7	39	theme	collagen	1252:1259	arg1	production					1288:1297	collagen and antiapoptotic proteins production	1252:1297	collagen and antiapoptotic proteins production	1252:1297	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	1	40	dep	derivatives	44:54	arg1	derivatives					44:54	bulgaricus derivatives	33:54	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.	33:166	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	1	40	dep	derivatives	44:54	arg1	Manufacturing					125:137	Manufacturing	125:137	Manufacturing	125:137	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	1	40	dep	derivatives	44:54	arg1	healing					149:155	wound healing potential	143:165	wound healing potential	143:165	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	3	41	theme	ALG/HA	420:425	arg1	hydrogels					428:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels	359:436	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	4	42	theme	different	572:580	arg1	strains					585:591	two different LB strains	568:591	two different LB strains	568:591	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	4	42	theme	different	572:580	arg1	LB1932					605:610	LB1932	605:610	LB1932	605:610	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	4	42	theme	different	572:580	arg1	LB1865					594:599	LB1865	594:599	LB1865	594:599	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	3	43	theme	first	322:326	arg1	time					328:331	the first time	318:331	the first time	318:331	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	6	44	theme	quantifiable	930:941	arg1	proliferation					901:913	cell proliferation	896:913	cell proliferation	896:913	The derivatives showed an ability to increase cell proliferation and migration, quantifiable between 10 and 20 % if compared to controls, with higher values for the derivatives obtained from the LB1932 strain.					
37076070	7	45	theme	liquid	1084:1089	arg1	spectrometry					1111:1122	liquid chromatography-mass spectrometry	1084:1122	liquid chromatography-mass spectrometry targeted protein biomarker analysis	1084:1158	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	1	46	theme	wound	143:147	arg1	derivatives					44:54	bulgaricus derivatives	33:54	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.	33:166	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	1	46	theme	wound	143:147	arg1	healing					149:155	wound healing potential	143:165	wound healing potential	143:165	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	7	47	theme	protein	1133:1139	arg1	analysis					1151:1158	liquid chromatography-mass spectrometry targeted protein biomarker analysis	1084:1158	liquid chromatography-mass spectrometry targeted protein biomarker analysis	1084:1158	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	2	48	theme	delbrueckii	244:254	arg1	subsp					256:260	Lactobacillus delbrueckii subsp	230:260	Lactobacillus delbrueckii subsp	230:260	Derivatives [i.e. proteins and exopolysaccharides (EPS)] from Lactobacillus delbrueckii subsp.					
37076070	3	49	from	potentials	494:503	arg1	applications					530:541	regenerative medicine applications	508:541	regenerative medicine applications	508:541	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	6	50	theme	higher	993:998	arg1	values					1000:1005	higher values	993:1005	higher values for the derivatives obtained from the LB1932 strain	993:1057	The derivatives showed an ability to increase cell proliferation and migration, quantifiable between 10 and 20 % if compared to controls, with higher values for the derivatives obtained from the LB1932 strain.					
37076070	7	51	theme	chromatography-mass	1091:1109	arg1	spectrometry					1111:1122	liquid chromatography-mass spectrometry	1084:1122	liquid chromatography-mass spectrometry targeted protein biomarker analysis	1084:1158	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	2	52	theme	Lactobacillus	230:242	arg1	subsp					256:260	Lactobacillus delbrueckii subsp	230:260	Lactobacillus delbrueckii subsp	230:260	Derivatives [i.e. proteins and exopolysaccharides (EPS)] from Lactobacillus delbrueckii subsp.					
37076070	1	53	theme	bulgaricus	33:42	arg1	derivatives					44:54	bulgaricus derivatives	33:54	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.	33:166	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	1	53	theme	bulgaricus	33:42	arg1	Manufacturing					125:137	Manufacturing	125:137	Manufacturing	125:137	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	1	53	theme	bulgaricus	33:42	arg1	healing					149:155	wound healing potential	143:165	wound healing potential	143:165	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	1	54	theme	potential	157:165	arg1	derivatives					44:54	bulgaricus derivatives	33:54	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.	33:166	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	1	54	theme	potential	157:165	arg1	healing					149:155	wound healing potential	143:165	wound healing potential	143:165	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	3	55	with	biomaterials	464:475	arg1	potentials					494:503	therapeutic potentials	482:503	therapeutic potentials in regenerative medicine applications	482:541	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	3	56	theme	medicine	521:528	arg1	applications					530:541	regenerative medicine applications	508:541	regenerative medicine applications	508:541	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	3	57	theme	regenerative	508:519	arg1	applications					530:541	regenerative medicine applications	508:541	regenerative medicine applications	508:541	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	4	58	from	cytotoxicity	657:668	arg1	migration					702:710	migration	702:710	migration	702:710	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	4	58	from	cytotoxicity	657:668	arg1	fibroblast					721:730	human fibroblast	715:730	human fibroblast	715:730	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	4	58	from	cytotoxicity	657:668	arg1	proliferation					684:696	proliferation	684:696	proliferation	684:696	Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast.					
37076070	3	59	theme	therapeutic	482:492	arg1	potentials					494:503	therapeutic potentials	482:503	therapeutic potentials in regenerative medicine applications	482:541	bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications.					
37076070	5	60	theme	human	832:836	arg1	fibroblast					838:847	the human fibroblast	828:847	the human fibroblast	828:847	EPS received particular attention as showing relevant dose-dependent cytocompatibility against the human fibroblast.					
37076070	1	61	theme	3D	60:61	arg1	hydrogels					114:122	3D printed alginate/hyaluronic acid self-crosslinking hydrogels	60:122	3D printed alginate/hyaluronic acid self-crosslinking hydrogels	60:122	bulgaricus derivatives for 3D printed alginate/hyaluronic acid self-crosslinking hydrogels: Manufacturing and wound healing potential.					
37076070	7	62	theme	antiapoptotic	1265:1277	arg1	proteins					1279:1286	antiapoptotic proteins	1265:1286	antiapoptotic proteins	1265:1286	These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production.					
37076070	5	63	theme	dose-dependent	787:800	arg1	cytocompatibility					802:818	relevant dose-dependent cytocompatibility	778:818	relevant dose-dependent cytocompatibility	778:818	EPS received particular attention as showing relevant dose-dependent cytocompatibility against the human fibroblast.					
35298549	8	0	theme	above	1492:1496	arg1	conclusion					1498:1507	the above conclusion	1488:1507	the above conclusion	1488:1507	Metagenomic data also corroborated the above conclusion.					
35298549	7	1	theme	coliforms	1333:1341	arg1	coliforms					1333:1341	coliforms	1333:1341	coliforms	1333:1341	Both phytobiotic-prebiotic mixture and ZnO were able to significantly reduce (P<0.05) the amount of coliforms after weaning, even though ZnO reduced the amount of coliforms more efficiently than the trial feed additive, it also reduced the amount of potentially beneficial bacteria.					
35298549	7	1	theme	coliforms	1333:1341	arg1	amount					1323:1328	the amount	1319:1328	the amount of coliforms	1319:1341	Both phytobiotic-prebiotic mixture and ZnO were able to significantly reduce (P<0.05) the amount of coliforms after weaning, even though ZnO reduced the amount of coliforms more efficiently than the trial feed additive, it also reduced the amount of potentially beneficial bacteria.					
35298549	4	2	theme	feed	774:777	arg1	supplement					779:788	no feed supplement	771:788	no feed supplement	771:788	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	4	2	theme	feed	774:777	arg1	control					762:768	a negative control	751:768	a negative control (no feed supplement)	751:789	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	6	3	theme	feed	1037:1040	arg1	ratio					1053:1057	The feed conversion ratio	1033:1057	The feed conversion ratio of the phytobiotic-prebiotic supplemented group	1033:1105	The feed conversion ratio of the phytobiotic-prebiotic supplemented group was significantly improved (P<0.05) compared to both controls.					
35298549	9	4	theme	positive	1527:1534	arg1	groups					1544:1549	the trial and positive control groups	1513:1549	groups	1544:1549	In the trial and positive control groups, the relative abundance of Enterobacteriaceae decreased by 85 and 88% between 3 weeks and 6 weeks of age, while in the negative control group a slight increase occurred.					
35298549	11	5	theme	diarrhea	2023:2030	arg1	prevention					1996:2005	the prevention	1992:2005	the prevention of post weaning diarrhea	1992:2030	In summary, this study demonstrated that a phytobiotic-prebiotic feed additive may be a real alternative to ZnO for the prevention of post weaning diarrhea and promote the development of a balanced gut system.					
35298549	10	6	theme	trial	1764:1768	arg1	group					1770:1774	the trial group	1760:1774	the trial group (29.98%)	1760:1783	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	10	6	theme	trial	1764:1768	arg1	%					1782:1782	29.98%	1777:1782	29.98%	1777:1782	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	4	7	theme	negative	753:760	arg1	supplement					779:788	no feed supplement	771:788	no feed supplement	771:788	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	4	7	theme	negative	753:760	arg1	control					762:768	a negative control	751:768	a negative control (no feed supplement)	751:789	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	7	8	theme	coliforms	1270:1278	arg1	coliforms					1270:1278	coliforms	1270:1278	coliforms	1270:1278	Both phytobiotic-prebiotic mixture and ZnO were able to significantly reduce (P<0.05) the amount of coliforms after weaning, even though ZnO reduced the amount of coliforms more efficiently than the trial feed additive, it also reduced the amount of potentially beneficial bacteria.					
35298549	7	8	theme	coliforms	1270:1278	arg1	P<0.05					1248:1253	P<0.05	1248:1253	P<0.05	1248:1253	Both phytobiotic-prebiotic mixture and ZnO were able to significantly reduce (P<0.05) the amount of coliforms after weaning, even though ZnO reduced the amount of coliforms more efficiently than the trial feed additive, it also reduced the amount of potentially beneficial bacteria.					
35298549	7	8	theme	coliforms	1270:1278	arg1	amount					1260:1265	the amount	1256:1265	(P<0.05) the amount of coliforms	1247:1278	Both phytobiotic-prebiotic mixture and ZnO were able to significantly reduce (P<0.05) the amount of coliforms after weaning, even though ZnO reduced the amount of coliforms more efficiently than the trial feed additive, it also reduced the amount of potentially beneficial bacteria.					
35298549	11	9	theme	post	2010:2013	arg1	diarrhea					2023:2030	post weaning diarrhea	2010:2030	post weaning diarrhea	2010:2030	In summary, this study demonstrated that a phytobiotic-prebiotic feed additive may be a real alternative to ZnO for the prevention of post weaning diarrhea and promote the development of a balanced gut system.					
35298549	1	10	theme	beneficial	172:181	arg1	effects					183:189	beneficial effects	172:189	beneficial effects	172:189	A wide range of phytobiotic feed additives are available on the market claiming to have beneficial effects on the growth of the host animal and to promote the development of a balanced microflora.					
35298549	5	11	dep	weight	873:878	arg1	the					858:860	the	858:860	the	858:860	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	5	12	theme	control	929:935	arg1	groups					937:942	the trial and positive control groups	906:942	groups	937:942	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	9	13	theme	Enterobacteriaceae	1578:1595	arg1	abundance					1565:1573	the relative abundance	1552:1573	the relative abundance of Enterobacteriaceae	1552:1595	In the trial and positive control groups, the relative abundance of Enterobacteriaceae decreased by 85 and 88% between 3 weeks and 6 weeks of age, while in the negative control group a slight increase occurred.					
35298549	10	14	dep	negative	1824:1831	arg1	%					1839:1839	22.45%	1834:1839	22.45%	1834:1839	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	6	15	theme	supplemented	1088:1099	arg1	group					1101:1105	the phytobiotic-prebiotic supplemented group	1062:1105	the phytobiotic-prebiotic supplemented group	1062:1105	The feed conversion ratio of the phytobiotic-prebiotic supplemented group was significantly improved (P<0.05) compared to both controls.					
35298549	1	16	theme	feed	112:115	arg1	additives					117:125	phytobiotic feed additives	100:125	phytobiotic feed additives	100:125	A wide range of phytobiotic feed additives are available on the market claiming to have beneficial effects on the growth of the host animal and to promote the development of a balanced microflora.					
35298549	3	17	theme	high	597:600	arg1	doses					602:606	high doses	597:606	high doses of zinc oxide (ZnO)	597:626	Post weaning diarrhea causes significant losses for the producers, most commonly it is prevented by feeding high doses of zinc oxide (ZnO).					
35298549	2	18	theme	weaning	472:478	arg1	piglets					480:486	weaning piglets	472:486	weaning piglets	472:486	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	9	19	theme	relative	1556:1563	arg1	abundance					1565:1573	the relative abundance	1552:1573	the relative abundance of Enterobacteriaceae	1552:1595	In the trial and positive control groups, the relative abundance of Enterobacteriaceae decreased by 85 and 88% between 3 weeks and 6 weeks of age, while in the negative control group a slight increase occurred.					
35298549	7	20	theme	beneficial	1432:1441	arg1	bacteria					1443:1450	potentially beneficial bacteria	1420:1450	potentially beneficial bacteria	1420:1450	Both phytobiotic-prebiotic mixture and ZnO were able to significantly reduce (P<0.05) the amount of coliforms after weaning, even though ZnO reduced the amount of coliforms more efficiently than the trial feed additive, it also reduced the amount of potentially beneficial bacteria.					
35298549	2	21	theme	wheat	374:378	arg1	germ					380:383	wheat germ	374:383	wheat germ	374:383	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	4	22	dep	in	797:798	arg1	vivo					800:803	vivo	800:803	vivo	800:803	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	11	23	theme	balanced	2065:2072	arg1	system					2078:2083	a balanced gut system	2063:2083	a balanced gut system	2063:2083	In summary, this study demonstrated that a phytobiotic-prebiotic feed additive may be a real alternative to ZnO for the prevention of post weaning diarrhea and promote the development of a balanced gut system.					
35298549	4	24	theme	kg	684:685	arg1	T-1					687:689	1 kg T-1	682:689	1 kg T-1	682:689	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	4	24	theme	kg	684:685	arg1	additive					672:679	additive	672:679	additive	672:679	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	2	25	theme	curcumin	364:371	arg1	mixture					353:359	the phytobiotic-prebiotic mixture	327:359	the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory	327:396	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	1	26	theme	wide	86:89	arg1	range					91:95	A wide range	84:95	A wide range of phytobiotic feed additives	84:125	A wide range of phytobiotic feed additives are available on the market claiming to have beneficial effects on the growth of the host animal and to promote the development of a balanced microflora.					
35298549	1	26	theme	wide	86:89	arg1	available					131:139	available	131:139	available	131:139	A wide range of phytobiotic feed additives are available on the market claiming to have beneficial effects on the growth of the host animal and to promote the development of a balanced microflora.					
35298549	9	27	theme	age	1652:1654	arg1	weeks					1631:1635	3 weeks	1629:1635	3 weeks	1629:1635	In the trial and positive control groups, the relative abundance of Enterobacteriaceae decreased by 85 and 88% between 3 weeks and 6 weeks of age, while in the negative control group a slight increase occurred.					
35298549	9	27	theme	age	1652:1654	arg1	weeks					1643:1647	6 weeks	1641:1647	6 weeks	1641:1647	In the trial and positive control groups, the relative abundance of Enterobacteriaceae decreased by 85 and 88% between 3 weeks and 6 weeks of age, while in the negative control group a slight increase occurred.					
35298549	10	28	from	weeks	1862:1866	arg1	abundant					1748:1755	abundant	1748:1755	abundant	1748:1755	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	2	29	theme	chicory	390:396	arg1	mixture					353:359	the phytobiotic-prebiotic mixture	327:359	the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory	327:396	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	7	30	theme	phytobiotic-prebiotic	1175:1195	arg1	mixture					1197:1203	phytobiotic-prebiotic mixture	1175:1203	phytobiotic-prebiotic mixture	1175:1203	Both phytobiotic-prebiotic mixture and ZnO were able to significantly reduce (P<0.05) the amount of coliforms after weaning, even though ZnO reduced the amount of coliforms more efficiently than the trial feed additive, it also reduced the amount of potentially beneficial bacteria.					
35298549	2	31	theme	intestinal	435:444	arg1	microflora					446:455	the intestinal microflora	431:455	the intestinal microflora composition of weaning piglets	431:486	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	3	32	theme	oxide	616:620	arg1	doses					602:606	high doses	597:606	high doses of zinc oxide (ZnO)	597:626	Post weaning diarrhea causes significant losses for the producers, most commonly it is prevented by feeding high doses of zinc oxide (ZnO).					
35298549	4	33	theme	T-1	735:737	arg1	ZnO					739:741	3.1 kg T-1 ZnO	728:741	3.1 kg T-1 ZnO	728:741	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	4	33	theme	T-1	735:737	arg1	control					719:725	a positive control	708:725	a positive control (3.1 kg T-1 ZnO)	708:742	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	1	34	theme	microflora	269:278	arg1	development					243:253	the development	239:253	the development of a balanced microflora	239:278	A wide range of phytobiotic feed additives are available on the market claiming to have beneficial effects on the growth of the host animal and to promote the development of a balanced microflora.					
35298549	5	35	theme	daily	892:896	arg1	gain					898:901	average daily gain	884:901	average daily gain	884:901	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	5	36	dep	significantly	968:980	arg1	P<0.05					983:988	P<0.05	983:988	P<0.05	983:988	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	0	37	theme	intestinal	41:50	arg1	microbiota					52:61	balanced intestinal microbiota	32:61	balanced intestinal microbiota for weaning piglets	32:81	Alternative to ZnO to establish balanced intestinal microbiota for weaning piglets.					
35298549	9	38	theme	control	1679:1685	arg1	group					1687:1691	the negative control group	1666:1691	the negative control group	1666:1691	In the trial and positive control groups, the relative abundance of Enterobacteriaceae decreased by 85 and 88% between 3 weeks and 6 weeks of age, while in the negative control group a slight increase occurred.					
35298549	10	39	dep	abundant	1748:1755	arg1	positive					1797:1804	positive	1797:1804	positive	1797:1804	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	10	39	dep	abundant	1748:1755	arg1	%					1811:1811	8.67%	1807:1811	8.67%	1807:1811	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	10	39	dep	abundant	1748:1755	arg1	groups					1850:1855	the negative (22.45%) control groups	1820:1855	the negative (22.45%) control groups	1820:1855	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	5	40	theme	body	868:871	arg1	weight					873:878	final body weight	862:878	final body weight	862:878	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	8	41	theme	Metagenomic	1453:1463	arg1	data					1465:1468	Metagenomic data	1453:1468	Metagenomic data	1453:1468	Metagenomic data also corroborated the above conclusion.					
35298549	5	42	theme	better	991:996	arg1	results					998:1004	significantly (P<0.05) better results	968:1004	significantly (P<0.05) better results	968:1004	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	3	43	theme	Post	489:492	arg1	diarrhea					502:509	Post weaning diarrhea	489:509	Post weaning diarrhea	489:509	Post weaning diarrhea causes significant losses for the producers, most commonly it is prevented by feeding high doses of zinc oxide (ZnO).					
35298549	1	44	theme	host	212:215	arg1	animal					217:222	the host animal	208:222	the host animal	208:222	A wide range of phytobiotic feed additives are available on the market claiming to have beneficial effects on the growth of the host animal and to promote the development of a balanced microflora.					
35298549	11	45	theme	system	2078:2083	arg1	development					2048:2058	the development	2044:2058	the development of a balanced gut system	2044:2083	In summary, this study demonstrated that a phytobiotic-prebiotic feed additive may be a real alternative to ZnO for the prevention of post weaning diarrhea and promote the development of a balanced gut system.					
35298549	3	46	theme	significant	518:528	arg1	losses					530:535	significant losses	518:535	significant losses for the producers	518:553	Post weaning diarrhea causes significant losses for the producers, most commonly it is prevented by feeding high doses of zinc oxide (ZnO).					
35298549	4	47	theme	no	771:772	arg1	supplement					779:788	no feed supplement	771:788	no feed supplement	771:788	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	4	47	theme	no	771:772	arg1	control					762:768	a negative control	751:768	a negative control (no feed supplement)	751:789	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	2	48	from	effects	316:322	arg1	performance					412:422	the growth performance	401:422	the growth performance	401:422	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	2	48	from	effects	316:322	arg1	composition					457:467	the intestinal microflora composition	431:467	the intestinal microflora composition of weaning piglets	431:486	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	11	49	theme	weaning	2015:2021	arg1	diarrhea					2023:2030	post weaning diarrhea	2010:2030	post weaning diarrhea	2010:2030	In summary, this study demonstrated that a phytobiotic-prebiotic feed additive may be a real alternative to ZnO for the prevention of post weaning diarrhea and promote the development of a balanced gut system.					
35298549	9	50	theme	control	1536:1542	arg1	groups					1544:1549	the trial and positive control groups	1513:1549	groups	1544:1549	In the trial and positive control groups, the relative abundance of Enterobacteriaceae decreased by 85 and 88% between 3 weeks and 6 weeks of age, while in the negative control group a slight increase occurred.					
35298549	10	51	theme	age	1871:1873	arg1	weeks					1862:1866	6 weeks	1860:1866	6 weeks of age	1860:1873	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	11	52	theme	real	1964:1967	arg1	alternative					1969:1979	a real alternative	1962:1979	a real alternative to ZnO for the prevention of post weaning diarrhea	1962:2030	In summary, this study demonstrated that a phytobiotic-prebiotic feed additive may be a real alternative to ZnO for the prevention of post weaning diarrhea and promote the development of a balanced gut system.					
35298549	11	52	theme	real	1964:1967	arg1	additive					1946:1953	additive	1946:1953	additive	1946:1953	In summary, this study demonstrated that a phytobiotic-prebiotic feed additive may be a real alternative to ZnO for the prevention of post weaning diarrhea and promote the development of a balanced gut system.					
35298549	2	53	theme	present	285:291	arg1	study					293:297	The present study	281:297	The present study	281:297	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	10	54	from	abundant	1748:1755	arg1	group					1770:1774	the trial group	1760:1774	the trial group (29.98%)	1760:1783	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	10	54	from	abundant	1748:1755	arg1	%					1782:1782	29.98%	1777:1782	29.98%	1777:1782	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	4	55	theme	in	797:798	arg1	trial					812:816	an in vivo animal trial	794:816	an in vivo animal trial	794:816	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	5	56	theme	groups	937:942	arg1	weight					873:878	final body weight	862:878	final body weight	862:878	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	5	56	theme	groups	937:942	arg1	gain					898:901	average daily gain	884:901	average daily gain	884:901	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	10	57	theme	control	1842:1848	arg1	groups					1850:1855	the negative (22.45%) control groups	1820:1855	the negative (22.45%) control groups	1820:1855	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	4	58	theme	animal	805:810	arg1	trial					812:816	an in vivo animal trial	794:816	an in vivo animal trial	794:816	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	6	59	theme	group	1101:1105	arg1	ratio					1053:1057	The feed conversion ratio	1033:1057	The feed conversion ratio of the phytobiotic-prebiotic supplemented group	1033:1105	The feed conversion ratio of the phytobiotic-prebiotic supplemented group was significantly improved (P<0.05) compared to both controls.					
35298549	1	60	theme	phytobiotic	100:110	arg1	additives					117:125	phytobiotic feed additives	100:125	phytobiotic feed additives	100:125	A wide range of phytobiotic feed additives are available on the market claiming to have beneficial effects on the growth of the host animal and to promote the development of a balanced microflora.					
35298549	10	61	from	group	1770:1774	arg1	abundant					1748:1755	abundant	1748:1755	abundant	1748:1755	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	6	62	theme	phytobiotic-prebiotic	1066:1086	arg1	group					1101:1105	the phytobiotic-prebiotic supplemented group	1062:1105	the phytobiotic-prebiotic supplemented group	1062:1105	The feed conversion ratio of the phytobiotic-prebiotic supplemented group was significantly improved (P<0.05) compared to both controls.					
35298549	5	63	theme	trial	910:914	arg1	weight					873:878	final body weight	862:878	final body weight	862:878	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	5	63	theme	trial	910:914	arg1	gain					898:901	average daily gain	884:901	average daily gain	884:901	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	1	64	theme	additives	117:125	arg1	range					91:95	A wide range	84:95	A wide range of phytobiotic feed additives	84:125	A wide range of phytobiotic feed additives are available on the market claiming to have beneficial effects on the growth of the host animal and to promote the development of a balanced microflora.					
35298549	1	64	theme	additives	117:125	arg1	available					131:139	available	131:139	available	131:139	A wide range of phytobiotic feed additives are available on the market claiming to have beneficial effects on the growth of the host animal and to promote the development of a balanced microflora.					
35298549	7	65	theme	bacteria	1443:1450	arg1	amount					1410:1415	the amount	1406:1415	the amount of potentially beneficial bacteria	1406:1450	Both phytobiotic-prebiotic mixture and ZnO were able to significantly reduce (P<0.05) the amount of coliforms after weaning, even though ZnO reduced the amount of coliforms more efficiently than the trial feed additive, it also reduced the amount of potentially beneficial bacteria.					
35298549	7	65	theme	bacteria	1443:1450	arg1	bacteria					1443:1450	potentially beneficial bacteria	1420:1450	potentially beneficial bacteria	1420:1450	Both phytobiotic-prebiotic mixture and ZnO were able to significantly reduce (P<0.05) the amount of coliforms after weaning, even though ZnO reduced the amount of coliforms more efficiently than the trial feed additive, it also reduced the amount of potentially beneficial bacteria.					
35298549	4	66	theme	additive	672:679	arg1	effect					633:638	The effect	629:638	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1)	629:690	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	0	67	theme	weaning	67:73	arg1	piglets					75:81	weaning piglets	67:81	weaning piglets	67:81	Alternative to ZnO to establish balanced intestinal microbiota for weaning piglets.					
35298549	5	68	theme	positive	920:927	arg1	groups					937:942	the trial and positive control groups	906:942	groups	937:942	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	6	69	theme	conversion	1042:1051	arg1	ratio					1053:1057	The feed conversion ratio	1033:1057	The feed conversion ratio of the phytobiotic-prebiotic supplemented group	1033:1105	The feed conversion ratio of the phytobiotic-prebiotic supplemented group was significantly improved (P<0.05) compared to both controls.					
35298549	2	70	theme	microflora	446:455	arg1	composition					457:467	the intestinal microflora composition	431:467	the intestinal microflora composition of weaning piglets	431:486	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	10	71	theme	negative	1824:1831	arg1	groups					1850:1855	the negative (22.45%) control groups	1820:1855	the negative (22.45%) control groups	1820:1855	Lactobacillaceae were more abundant in the trial group (29.98%) than in the positive (8.67%) or in the negative (22.45%) control groups at 6 weeks of age.					
35298549	0	72	dep	ZnO	15:17	arg1	establish					22:30	establish	22:30	to establish balanced intestinal microbiota for weaning piglets	19:81	Alternative to ZnO to establish balanced intestinal microbiota for weaning piglets.					
35298549	5	73	theme	significant	832:842	arg1	difference					844:853	no significant difference	829:853	no significant difference	829:853	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	2	74	theme	piglets	480:486	arg1	composition					457:467	the intestinal microflora composition	431:467	the intestinal microflora composition of weaning piglets	431:486	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	2	75	theme	growth	405:410	arg1	performance					412:422	the growth performance	401:422	the growth performance	401:422	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	3	76	theme	zinc	611:614	arg1	ZnO					623:625	ZnO	623:625	ZnO	623:625	Post weaning diarrhea causes significant losses for the producers, most commonly it is prevented by feeding high doses of zinc oxide (ZnO).					
35298549	3	76	theme	zinc	611:614	arg1	oxide					616:620	zinc oxide	611:620	zinc oxide (ZnO)	611:626	Post weaning diarrhea causes significant losses for the producers, most commonly it is prevented by feeding high doses of zinc oxide (ZnO).					
35298549	2	77	theme	mixture	353:359	arg1	effects					316:322	the effects	312:322	the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets	312:486	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	0	78	theme	balanced	32:39	arg1	microbiota					52:61	balanced intestinal microbiota	32:61	balanced intestinal microbiota for weaning piglets	32:81	Alternative to ZnO to establish balanced intestinal microbiota for weaning piglets.					
35298549	1	79	theme	balanced	260:267	arg1	microflora					269:278	a balanced microflora	258:278	a balanced microflora	258:278	A wide range of phytobiotic feed additives are available on the market claiming to have beneficial effects on the growth of the host animal and to promote the development of a balanced microflora.					
35298549	5	80	theme	average	884:890	arg1	gain					898:901	average daily gain	884:901	average daily gain	884:901	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	4	81	theme	kg	732:733	arg1	ZnO					739:741	3.1 kg T-1 ZnO	728:741	3.1 kg T-1 ZnO	728:741	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	4	81	theme	kg	732:733	arg1	control					719:725	a positive control	708:725	a positive control (3.1 kg T-1 ZnO)	708:742	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	2	82	theme	germ	380:383	arg1	mixture					353:359	the phytobiotic-prebiotic mixture	327:359	the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory	327:396	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	9	83	theme	negative	1670:1677	arg1	group					1687:1691	the negative control group	1666:1691	the negative control group	1666:1691	In the trial and positive control groups, the relative abundance of Enterobacteriaceae decreased by 85 and 88% between 3 weeks and 6 weeks of age, while in the negative control group a slight increase occurred.					
35298549	5	84	theme	negative	1015:1022	arg1	control					1024:1030	the negative control	1011:1030	the negative control	1011:1030	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	5	85	theme	final	862:866	arg1	weight					873:878	final body weight	862:878	final body weight	862:878	There was no significant difference in the final body weight and average daily gain of the trial and positive control groups, and both groups showed significantly (P<0.05) better results than the negative control.					
35298549	11	86	theme	gut	2074:2076	arg1	system					2078:2083	a balanced gut system	2063:2083	a balanced gut system	2063:2083	In summary, this study demonstrated that a phytobiotic-prebiotic feed additive may be a real alternative to ZnO for the prevention of post weaning diarrhea and promote the development of a balanced gut system.					
35298549	4	87	theme	positive	710:717	arg1	ZnO					739:741	3.1 kg T-1 ZnO	728:741	3.1 kg T-1 ZnO	728:741	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	4	87	theme	positive	710:717	arg1	control					719:725	a positive control	708:725	a positive control (3.1 kg T-1 ZnO)	708:742	The effect of a phytobiotic-prebiotic feed additive (1 kg T-1) was compared to a positive control (3.1 kg T-1 ZnO) and to a negative control (no feed supplement) in an in vivo animal trial.					
35298549	2	88	theme	phytobiotic-prebiotic	331:351	arg1	mixture					353:359	the phytobiotic-prebiotic mixture	327:359	the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory	327:396	The present study investigated the effects of the phytobiotic-prebiotic mixture of curcumin, wheat germ, and chicory on the growth performance and on the intestinal microflora composition of weaning piglets.					
35298549	6	89	dep	improved	1125:1132	arg1	P<0.05					1135:1140	P<0.05	1135:1140	P<0.05	1135:1140	The feed conversion ratio of the phytobiotic-prebiotic supplemented group was significantly improved (P<0.05) compared to both controls.					
35298549	9	90	theme	slight	1695:1700	arg1	increase					1702:1709	a slight increase	1693:1709	a slight increase	1693:1709	In the trial and positive control groups, the relative abundance of Enterobacteriaceae decreased by 85 and 88% between 3 weeks and 6 weeks of age, while in the negative control group a slight increase occurred.					
35298549	3	91	theme	weaning	494:500	arg1	diarrhea					502:509	Post weaning diarrhea	489:509	Post weaning diarrhea	489:509	Post weaning diarrhea causes significant losses for the producers, most commonly it is prevented by feeding high doses of zinc oxide (ZnO).					
35298549	1	92	theme	animal	217:222	arg1	growth					198:203	the growth	194:203	the growth of the host animal	194:222	A wide range of phytobiotic feed additives are available on the market claiming to have beneficial effects on the growth of the host animal and to promote the development of a balanced microflora.					
35869741	5	0	dep	CI	1237:1238	arg1	p = 0.60					1251:1258	p = 0.60	1251:1258	p = 0.60	1251:1258	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	0	1	theme	AFFIRM-AHF	159:168	arg1	trial					170:174	the AFFIRM-AHF trial	155:174	the AFFIRM-AHF trial	155:174	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	4	2	theme	HF	873:874	arg1	subgroup					876:883	the ischaemic versus non-ischaemic HF subgroup	838:883	the ischaemic versus non-ischaemic HF subgroup	838:883	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	8	3	theme	failure	1625:1631	arg1	hospitalisations					1633:1648	Heart failure hospitalisations	1619:1648	Heart failure hospitalisations	1619:1648	CONCLUSIONS Heart failure hospitalisations and cardiovascular deaths occurred at a higher rate in patients with ishaemic versus those with non-ischaemic HF and were reduced by FCM versus placebo only in ischaemic patients.					
35869741	1	4	theme	failure	251:257	arg1	hospitalisations					264:279	heart failure (HF) hospitalisations	245:279	heart failure (HF) hospitalisations	245:279	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	5	5	dep	65.3	1026:1029	arg1	p = 0.007					1158:1166	p = 0.007	1158:1166	p = 0.007	1158:1166	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	5	dep	65.3	1026:1029	arg1	0.47-0.89					1147:1155	0.47-0.89	1147:1155	0.47-0.89	1147:1155	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	6	from	58.3	1173:1176	arg1	subgroup					1214:1221	the non-ischaemic HF subgroup	1193:1221	the non-ischaemic HF subgroup	1193:1221	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	0	7	theme	heart	126:130	arg1	failure					132:138	acute heart failure	120:138	acute heart failure	120:138	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	1	8	theme	HF	378:379	arg1	episode					381:387	an acute HF episode	369:387	an acute HF episode	369:387	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	3	9	theme	ischaemic	578:586	arg1	HF					588:589	ischaemic HF	578:589	ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation)	578:728	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	8	10	theme	higher	1690:1695	arg1	rate					1697:1700	a higher rate	1688:1700	a higher rate in patients with ishaemic versus those with non-ischaemic HF	1688:1761	CONCLUSIONS Heart failure hospitalisations and cardiovascular deaths occurred at a higher rate in patients with ishaemic versus those with non-ischaemic HF and were reduced by FCM versus placebo only in ischaemic patients.					
35869741	1	11	theme	intravenous	197:207	arg1	AFFIRM-AHF					185:194	AFFIRM-AHF	185:194	AFFIRM-AHF	185:194	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	1	11	theme	intravenous	197:207	arg1	FCM					232:234	FCM	232:234	FCM	232:234	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	1	11	theme	intravenous	197:207	arg1	carboxymaltose					216:229	intravenous ferric carboxymaltose	197:229	intravenous ferric carboxymaltose (FCM)	197:235	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	9	12	theme	aetiology	1879:1887	arg1	role					1871:1874	the role	1867:1874	the role of aetiology in FCM efficacy	1867:1903	Further studies are needed to assess the role of aetiology in FCM efficacy.					
35869741	8	13	with	patients	1705:1712	arg1	ishaemic					1719:1726	ishaemic	1719:1726	ishaemic	1719:1726	CONCLUSIONS Heart failure hospitalisations and cardiovascular deaths occurred at a higher rate in patients with ishaemic versus those with non-ischaemic HF and were reduced by FCM versus placebo only in ischaemic patients.					
35869741	7	14	theme	Cardiomyopathy	1517:1530	arg1	Questionnaire					1532:1544	the 12-item Kansas City Cardiomyopathy Questionnaire	1493:1544	the 12-item Kansas City Cardiomyopathy Questionnaire	1493:1544	A nominal increase in quality of life, assessed using the 12-item Kansas City Cardiomyopathy Questionnaire, was observed with FCM versus placebo, within each subgroup.					
35869741	9	15	theme	FCM	1892:1894	arg1	efficacy					1896:1903	FCM efficacy	1892:1903	FCM efficacy	1892:1903	Further studies are needed to assess the role of aetiology in FCM efficacy.					
35869741	5	16	theme	primary	917:923	arg1	outcome					935:941	the primary composite outcome	913:941	the primary composite outcome of total HF hospitalisations	913:970	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	7	17	theme	Kansas	1505:1510	arg1	Questionnaire					1532:1544	the 12-item Kansas City Cardiomyopathy Questionnaire	1493:1544	the 12-item Kansas City Cardiomyopathy Questionnaire	1493:1544	A nominal increase in quality of life, assessed using the 12-item Kansas City Cardiomyopathy Questionnaire, was observed with FCM versus placebo, within each subgroup.					
35869741	2	18	theme	non-ischaemic	478:490	arg1	aetiology					495:503	ischaemic and non-ischaemic HF aetiology	464:503	ischaemic and non-ischaemic HF aetiology	464:503	This analysis explored the effects of FCM versus placebo in patients with ischaemic and non-ischaemic HF aetiology.					
35869741	3	19	from	AFFIRM-AHF	557:566	arg1	patients					543:550	1082 patients	538:550	1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF	538:758	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	6	20	theme	HF	1308:1309	arg1	aetiology					1311:1319	HF aetiology	1308:1319	HF aetiology	1308:1319	An interaction between HF aetiology and treatment effect was also observed for the secondary outcome of total HF hospitalisations (pinteraction = 0.038).					
35869741	1	21	theme	life	305:308	arg1	quality					294:300	quality	294:300	quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode	294:387	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	2	22	with	patients	450:457	arg1	aetiology					495:503	ischaemic and non-ischaemic HF aetiology	464:503	ischaemic and non-ischaemic HF aetiology	464:503	This analysis explored the effects of FCM versus placebo in patients with ischaemic and non-ischaemic HF aetiology.					
35869741	3	23	dep	patients	543:550	arg1	590					569:571	590	569:571	590	569:571	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	3	23	dep	patients	543:550	arg1	492					734:736	492	734:736	492	734:736	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	2	24	theme	ischaemic	464:472	arg1	aetiology					495:503	ischaemic and non-ischaemic HF aetiology	464:503	ischaemic and non-ischaemic HF aetiology	464:503	This analysis explored the effects of FCM versus placebo in patients with ischaemic and non-ischaemic HF aetiology.					
35869741	5	25	dep	[RR	1107:1109	arg1	[CI					1142:1144	0.65, 95% confidence interval [CI	1112:1144	0.65, 95% confidence interval [CI	1112:1144	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	26	theme	HF	1211:1212	arg1	subgroup					1214:1221	the non-ischaemic HF subgroup	1193:1221	the non-ischaemic HF subgroup	1193:1221	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	0	27	theme	carboxymaltose	68:81	arg1	efficacy					37:44	the efficacy	33:44	the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure	33:138	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	1	28	theme	placebo	317:323	arg1	quality					294:300	quality	294:300	quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode	294:387	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	5	29	theme	event	897:901	arg1	rates					903:907	Annualised event rates	886:907	Annualised event rates for the primary composite outcome of total HF hospitalisations	886:970	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	0	30	from	Impact	0:5	arg1	efficacy					37:44	the efficacy	33:44	the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure	33:138	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	3	31	dep	METHODS	506:512	arg1	included					529:536	included	529:536	included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF	529:758	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	1	32	theme	iron-deficient	328:341	arg1	patients					343:350	iron-deficient patients	328:350	iron-deficient patients stabilised after an acute HF episode	328:387	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	5	33	theme	hospitalisations	955:970	arg1	outcome					935:941	the primary composite outcome	913:941	the primary composite outcome of total HF hospitalisations	913:970	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	7	34	theme	life	1472:1475	arg1	quality					1461:1467	quality	1461:1467	quality of life	1461:1475	A nominal increase in quality of life, assessed using the 12-item Kansas City Cardiomyopathy Questionnaire, was observed with FCM versus placebo, within each subgroup.					
35869741	0	35	from	efficacy	37:44	arg1	patients					86:93	patients	86:93	patients with iron deficiency and acute heart failure	86:138	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	4	36	theme	male	780:783	arg1	sex					785:787	male sex	780:787	male sex	780:787	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	0	37	theme	iron	100:103	arg1	deficiency					105:114	iron deficiency	100:114	iron deficiency	100:114	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	5	38	theme	rate	1096:1099	arg1	subgroup					1086:1093	the ischaemic HF subgroup	1069:1093	the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI]	1069:1145	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	38	theme	rate	1096:1099	arg1	[RR					1107:1109	rate ratio [RR	1096:1109	rate ratio [RR	1096:1109	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	39	theme	cardiovascular	976:989	arg1	death					991:995	cardiovascular death	976:995	cardiovascular death with FCM versus placebo	976:1019	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	2	40	theme	FCM	428:430	arg1	effects					417:423	the effects	413:423	the effects of FCM versus placebo in patients with ischaemic and non-ischaemic HF aetiology	413:503	This analysis explored the effects of FCM versus placebo in patients with ischaemic and non-ischaemic HF aetiology.					
35869741	5	41	with	rates	903:907	arg1	FCM					1002:1004	FCM	1002:1004	FCM	1002:1004	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	41	with	rates	903:907	arg1	placebo					1013:1019	placebo	1013:1019	placebo	1013:1019	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	0	42	theme	ischaemic	10:18	arg1	aetiology					20:28	ischaemic aetiology	10:28	ischaemic aetiology	10:28	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	5	43	theme	total	946:950	arg1	hospitalisations					955:970	total HF hospitalisations	946:970	total HF hospitalisations	946:970	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	0	44	theme	intravenous	49:59	arg1	carboxymaltose					68:81	intravenous ferric carboxymaltose	49:81	intravenous ferric carboxymaltose	49:81	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	4	45	theme	history	809:815	arg1	prevalences					765:775	The prevalences	761:775	The prevalences of male sex, comorbidities, and history of HF	761:821	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	4	45	theme	history	809:815	arg1	higher					828:833	higher	828:833	higher	828:833	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	7	46	theme	nominal	1441:1447	arg1	increase					1449:1456	A nominal increase	1439:1456	A nominal increase	1439:1456	A nominal increase in quality of life, assessed using the 12-item Kansas City Cardiomyopathy Questionnaire, was observed with FCM versus placebo, within each subgroup.					
35869741	2	47	theme	placebo	439:445	arg1	effects					417:423	the effects	413:423	the effects of FCM versus placebo in patients with ischaemic and non-ischaemic HF aetiology	413:503	This analysis explored the effects of FCM versus placebo in patients with ischaemic and non-ischaemic HF aetiology.					
35869741	3	48	theme	ischaemic	625:633	arg1	aetiology					638:646	investigator-reported ischaemic HF aetiology	603:646	investigator-reported ischaemic HF aetiology	603:646	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	4	49	theme	HF	820:821	arg1	comorbidities					790:802	comorbidities	790:802	comorbidities	790:802	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	4	49	theme	HF	820:821	arg1	history					809:815	history	809:815	history of HF	809:821	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	4	49	theme	HF	820:821	arg1	sex					785:787	male sex	780:787	male sex	780:787	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	5	50	theme	confidence	1122:1131	arg1	[CI					1142:1144	0.65, 95% confidence interval [CI	1112:1144	0.65, 95% confidence interval [CI	1112:1144	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	3	51	theme	myocardial	667:676	arg1	infarction					678:687	acute myocardial infarction	661:687	acute myocardial infarction	661:687	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	1	52	theme	ferric	209:214	arg1	AFFIRM-AHF					185:194	AFFIRM-AHF	185:194	AFFIRM-AHF	185:194	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	1	52	theme	ferric	209:214	arg1	FCM					232:234	FCM	232:234	FCM	232:234	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	1	52	theme	ferric	209:214	arg1	carboxymaltose					216:229	intravenous ferric carboxymaltose	197:229	intravenous ferric carboxymaltose (FCM)	197:235	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	3	53	theme	prior	655:659	arg1	infarction					678:687	acute myocardial infarction	661:687	acute myocardial infarction	661:687	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	6	54	theme	HF	1395:1396	arg1	hospitalisations					1398:1413	total HF hospitalisations	1389:1413	total HF hospitalisations (pinteraction = 0.038)	1389:1436	An interaction between HF aetiology and treatment effect was also observed for the secondary outcome of total HF hospitalisations (pinteraction = 0.038).					
35869741	6	54	theme	HF	1395:1396	arg1	= 0.038					1429:1435	pinteraction = 0.038	1416:1435	pinteraction = 0.038	1416:1435	An interaction between HF aetiology and treatment effect was also observed for the secondary outcome of total HF hospitalisations (pinteraction = 0.038).					
35869741	5	55	with	death	991:995	arg1	FCM					1002:1004	FCM	1002:1004	FCM	1002:1004	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	55	with	death	991:995	arg1	placebo					1013:1019	placebo	1013:1019	placebo	1013:1019	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	3	56	theme	coronary	702:709	arg1	revascularisation					711:727	prior coronary revascularisation	696:727	prior coronary revascularisation	696:727	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	1	57	from	quality	294:300	arg1	patients					343:350	iron-deficient patients	328:350	iron-deficient patients stabilised after an acute HF episode	328:387	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	4	58	theme	ischaemic	842:850	arg1	subgroup					876:883	the ischaemic versus non-ischaemic HF subgroup	838:883	the ischaemic versus non-ischaemic HF subgroup	838:883	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	6	59	theme	secondary	1368:1376	arg1	outcome					1378:1384	the secondary outcome	1364:1384	the secondary outcome of total HF hospitalisations (pinteraction = 0.038)	1364:1436	An interaction between HF aetiology and treatment effect was also observed for the secondary outcome of total HF hospitalisations (pinteraction = 0.038).					
35869741	1	60	theme	heart	245:249	arg1	HF					260:261	HF	260:261	HF	260:261	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	1	60	theme	heart	245:249	arg1	failure					251:257	heart failure	245:257	heart failure (HF) hospitalisations	245:279	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	7	61	from	increase	1449:1456	arg1	quality					1461:1467	quality	1461:1467	quality of life	1461:1475	A nominal increase in quality of life, assessed using the 12-item Kansas City Cardiomyopathy Questionnaire, was observed with FCM versus placebo, within each subgroup.					
35869741	4	62	theme	non-ischaemic	859:871	arg1	subgroup					876:883	the ischaemic versus non-ischaemic HF subgroup	838:883	the ischaemic versus non-ischaemic HF subgroup	838:883	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	9	63	theme	Further	1830:1836	arg1	studies					1838:1844	Further studies	1830:1844	Further studies	1830:1844	Further studies are needed to assess the role of aetiology in FCM efficacy.					
35869741	8	64	theme	ischaemic	1810:1818	arg1	patients					1820:1827	ischaemic patients	1810:1827	ischaemic patients	1810:1827	CONCLUSIONS Heart failure hospitalisations and cardiovascular deaths occurred at a higher rate in patients with ishaemic versus those with non-ischaemic HF and were reduced by FCM versus placebo only in ischaemic patients.					
35869741	8	65	theme	Heart	1619:1623	arg1	failure					1625:1631	Heart failure	1619:1631	Heart failure hospitalisations	1619:1648	CONCLUSIONS Heart failure hospitalisations and cardiovascular deaths occurred at a higher rate in patients with ishaemic versus those with non-ischaemic HF and were reduced by FCM versus placebo only in ischaemic patients.					
35869741	8	66	from	rate	1697:1700	arg1	those					1735:1739	those	1735:1739	those	1735:1739	CONCLUSIONS Heart failure hospitalisations and cardiovascular deaths occurred at a higher rate in patients with ishaemic versus those with non-ischaemic HF and were reduced by FCM versus placebo only in ischaemic patients.					
35869741	8	66	from	rate	1697:1700	arg1	patients					1705:1712	patients	1705:1712	patients with ishaemic	1705:1726	CONCLUSIONS Heart failure hospitalisations and cardiovascular deaths occurred at a higher rate in patients with ishaemic versus those with non-ischaemic HF and were reduced by FCM versus placebo only in ischaemic patients.					
35869741	0	67	theme	acute	120:124	arg1	failure					132:138	acute heart failure	120:138	acute heart failure	120:138	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	0	68	with	patients	86:93	arg1	failure					132:138	acute heart failure	120:138	acute heart failure	120:138	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	0	68	with	patients	86:93	arg1	deficiency					105:114	iron deficiency	100:114	iron deficiency	100:114	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	3	69	theme	1082	538:541	arg1	patients					543:550	1082 patients	538:550	1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF	538:758	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	8	70	theme	cardiovascular	1654:1667	arg1	deaths					1669:1674	cardiovascular deaths	1654:1674	cardiovascular deaths	1654:1674	CONCLUSIONS Heart failure hospitalisations and cardiovascular deaths occurred at a higher rate in patients with ishaemic versus those with non-ischaemic HF and were reduced by FCM versus placebo only in ischaemic patients.					
35869741	3	71	dep	HF	588:589	arg1	defined					592:598	defined	592:598	defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation	592:727	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	3	72	theme	non-ischaemic	743:755	arg1	HF					757:758	non-ischaemic HF	743:758	non-ischaemic HF	743:758	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	1	73	theme	acute	372:376	arg1	episode					381:387	an acute HF episode	369:387	an acute HF episode	369:387	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	9	74	from	role	1871:1874	arg1	efficacy					1896:1903	FCM efficacy	1892:1903	FCM efficacy	1892:1903	Further studies are needed to assess the role of aetiology in FCM efficacy.					
35869741	8	75	dep	CONCLUSIONS	1607:1617	arg1	hospitalisations					1633:1648	Heart failure hospitalisations	1619:1648	Heart failure hospitalisations	1619:1648	CONCLUSIONS Heart failure hospitalisations and cardiovascular deaths occurred at a higher rate in patients with ishaemic versus those with non-ischaemic HF and were reduced by FCM versus placebo only in ischaemic patients.					
35869741	5	76	theme	HF	1083:1084	arg1	subgroup					1086:1093	the ischaemic HF subgroup	1069:1093	the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI]	1069:1145	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	76	theme	HF	1083:1084	arg1	[RR					1107:1109	rate ratio [RR	1096:1109	rate ratio [RR	1096:1109	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	7	77	theme	City	1512:1515	arg1	Questionnaire					1532:1544	the 12-item Kansas City Cardiomyopathy Questionnaire	1493:1544	the 12-item Kansas City Cardiomyopathy Questionnaire	1493:1544	A nominal increase in quality of life, assessed using the 12-item Kansas City Cardiomyopathy Questionnaire, was observed with FCM versus placebo, within each subgroup.					
35869741	5	78	theme	RR	1224:1225	arg1	58.3					1173:1176	58.3	1173:1176	58.3	1173:1176	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	78	theme	RR	1224:1225	arg1	CI					1237:1238	RR 1.11, 95% CI 0.75-1.66	1224:1248	CI	1237:1238	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	79	from	patient-years	1052:1064	arg1	subgroup					1086:1093	the ischaemic HF subgroup	1069:1093	the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI]	1069:1145	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	79	from	patient-years	1052:1064	arg1	[RR					1107:1109	rate ratio [RR	1096:1109	rate ratio [RR	1096:1109	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	0	80	dep	insights	141:148	arg1	Impact					0:5	Impact	0:5	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure	0:138	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	7	81	theme	12-item	1497:1503	arg1	Questionnaire					1532:1544	the 12-item Kansas City Cardiomyopathy Questionnaire	1493:1544	the 12-item Kansas City Cardiomyopathy Questionnaire	1493:1544	A nominal increase in quality of life, assessed using the 12-item Kansas City Cardiomyopathy Questionnaire, was observed with FCM versus placebo, within each subgroup.					
35869741	0	82	theme	ferric	61:66	arg1	carboxymaltose					68:81	intravenous ferric carboxymaltose	49:81	intravenous ferric carboxymaltose	49:81	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	1	83	from	AIMS	177:180	arg1	AFFIRM-AHF					185:194	AFFIRM-AHF	185:194	AFFIRM-AHF	185:194	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	1	83	from	AIMS	177:180	arg1	carboxymaltose					216:229	intravenous ferric carboxymaltose	197:229	intravenous ferric carboxymaltose (FCM)	197:235	AIMS In AFFIRM-AHF, intravenous ferric carboxymaltose (FCM) reduced heart failure (HF) hospitalisations and improved quality of life versus placebo in iron-deficient patients stabilised after an acute HF episode.					
35869741	2	84	from	effects	417:423	arg1	patients					450:457	patients	450:457	patients with ischaemic and non-ischaemic HF aetiology	450:503	This analysis explored the effects of FCM versus placebo in patients with ischaemic and non-ischaemic HF aetiology.					
35869741	5	85	theme	non-ischaemic	1197:1209	arg1	subgroup					1214:1221	the non-ischaemic HF subgroup	1193:1221	the non-ischaemic HF subgroup	1193:1221	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	86	theme	Annualised	886:895	arg1	rates					903:907	Annualised event rates	886:907	Annualised event rates for the primary composite outcome of total HF hospitalisations	886:970	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	87	theme	HF	952:953	arg1	hospitalisations					955:970	total HF hospitalisations	946:970	total HF hospitalisations	946:970	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	8	88	theme	non-ischaemic	1746:1758	arg1	HF					1760:1761	non-ischaemic HF	1746:1761	non-ischaemic HF	1746:1761	CONCLUSIONS Heart failure hospitalisations and cardiovascular deaths occurred at a higher rate in patients with ishaemic versus those with non-ischaemic HF and were reduced by FCM versus placebo only in ischaemic patients.					
35869741	2	89	theme	HF	492:493	arg1	aetiology					495:503	ischaemic and non-ischaemic HF aetiology	464:503	ischaemic and non-ischaemic HF aetiology	464:503	This analysis explored the effects of FCM versus placebo in patients with ischaemic and non-ischaemic HF aetiology.					
35869741	5	90	theme	ratio	1101:1105	arg1	subgroup					1086:1093	the ischaemic HF subgroup	1069:1093	the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI]	1069:1145	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	90	theme	ratio	1101:1105	arg1	[RR					1107:1109	rate ratio [RR	1096:1109	rate ratio [RR	1096:1109	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	91	theme	composite	925:933	arg1	outcome					935:941	the primary composite outcome	913:941	the primary composite outcome of total HF hospitalisations	913:970	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	92	theme	%	1235:1235	arg1	58.3					1173:1176	58.3	1173:1176	58.3	1173:1176	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	92	theme	%	1235:1235	arg1	CI					1237:1238	RR 1.11, 95% CI 0.75-1.66	1224:1248	CI	1237:1238	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	0	93	theme	aetiology	20:28	arg1	Impact					0:5	Impact	0:5	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure	0:138	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	7	94	located	observed	1551:1558	arg1	subgroup					1597:1604	each subgroup	1592:1604	each subgroup	1592:1604	A nominal increase in quality of life, assessed using the 12-item Kansas City Cardiomyopathy Questionnaire, was observed with FCM versus placebo, within each subgroup.					
35869741	7	94	located	observed	1551:1558	arg2	increase					1449:1456	A nominal increase	1439:1456	A nominal increase	1439:1456	A nominal increase in quality of life, assessed using the 12-item Kansas City Cardiomyopathy Questionnaire, was observed with FCM versus placebo, within each subgroup.					
35869741	0	95	from	trial	170:174	arg1	insights					141:148	insights	141:148	insights from the AFFIRM-AHF trial	141:174	Impact of ischaemic aetiology on the efficacy of intravenous ferric carboxymaltose in patients with iron deficiency and acute heart failure: insights from the AFFIRM-AHF trial.					
35869741	3	96	theme	HF	635:636	arg1	aetiology					638:646	investigator-reported ischaemic HF aetiology	603:646	investigator-reported ischaemic HF aetiology	603:646	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	3	97	theme	investigator-reported	603:623	arg1	aetiology					638:646	investigator-reported ischaemic HF aetiology	603:646	investigator-reported ischaemic HF aetiology	603:646	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	6	98	theme	treatment	1325:1333	arg1	effect					1335:1340	treatment effect	1325:1340	treatment effect	1325:1340	An interaction between HF aetiology and treatment effect was also observed for the secondary outcome of total HF hospitalisations (pinteraction = 0.038).					
35869741	5	99	theme	%	1120:1120	arg1	[CI					1142:1144	0.65, 95% confidence interval [CI	1112:1144	0.65, 95% confidence interval [CI	1112:1144	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	3	100	theme	acute	661:665	arg1	infarction					678:687	acute myocardial infarction	661:687	acute myocardial infarction	661:687	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	5	101	theme	ischaemic	1073:1081	arg1	subgroup					1086:1093	the ischaemic HF subgroup	1069:1093	the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI]	1069:1145	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	5	101	theme	ischaemic	1073:1081	arg1	[RR					1107:1109	rate ratio [RR	1096:1109	rate ratio [RR	1096:1109	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	6	102	theme	pinteraction	1416:1427	arg1	hospitalisations					1398:1413	total HF hospitalisations	1389:1413	total HF hospitalisations (pinteraction = 0.038)	1389:1436	An interaction between HF aetiology and treatment effect was also observed for the secondary outcome of total HF hospitalisations (pinteraction = 0.038).					
35869741	6	102	theme	pinteraction	1416:1427	arg1	= 0.038					1429:1435	pinteraction = 0.038	1416:1435	pinteraction = 0.038	1416:1435	An interaction between HF aetiology and treatment effect was also observed for the secondary outcome of total HF hospitalisations (pinteraction = 0.038).					
35869741	5	103	theme	interval	1133:1140	arg1	[CI					1142:1144	0.65, 95% confidence interval [CI	1112:1144	0.65, 95% confidence interval [CI	1112:1144	Annualised event rates for the primary composite outcome of total HF hospitalisations and cardiovascular death with FCM versus placebo were 65.3 versus 100.6 per 100 patient-years in the ischaemic HF subgroup (rate ratio [RR] 0.65, 95% confidence interval [CI] 0.47-0.89, p = 0.007) and 58.3 versus 52.5 in the non-ischaemic HF subgroup (RR 1.11, 95% CI 0.75-1.66, p = 0.60) (pinteraction = 0.039).					
35869741	4	104	theme	sex	785:787	arg1	prevalences					765:775	The prevalences	761:775	The prevalences of male sex, comorbidities, and history of HF	761:821	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	4	104	theme	sex	785:787	arg1	higher					828:833	higher	828:833	higher	828:833	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	6	105	theme	hospitalisations	1398:1413	arg1	outcome					1378:1384	the secondary outcome	1364:1384	the secondary outcome of total HF hospitalisations (pinteraction = 0.038)	1364:1436	An interaction between HF aetiology and treatment effect was also observed for the secondary outcome of total HF hospitalisations (pinteraction = 0.038).					
35869741	3	106	theme	prior	696:700	arg1	revascularisation					711:727	prior coronary revascularisation	696:727	prior coronary revascularisation	696:727	METHODS AND RESULTS We included 1082 patients from AFFIRM-AHF: 590 with ischaemic HF (defined as investigator-reported ischaemic HF aetiology and/or prior acute myocardial infarction and/or prior coronary revascularisation) and 492 with non-ischaemic HF.					
35869741	4	107	theme	comorbidities	790:802	arg1	prevalences					765:775	The prevalences	761:775	The prevalences of male sex, comorbidities, and history of HF	761:821	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	4	107	theme	comorbidities	790:802	arg1	higher					828:833	higher	828:833	higher	828:833	The prevalences of male sex, comorbidities, and history of HF were higher in the ischaemic versus non-ischaemic HF subgroup.					
35869741	6	108	theme	total	1389:1393	arg1	hospitalisations					1398:1413	total HF hospitalisations	1389:1413	total HF hospitalisations (pinteraction = 0.038)	1389:1436	An interaction between HF aetiology and treatment effect was also observed for the secondary outcome of total HF hospitalisations (pinteraction = 0.038).					
35869741	6	108	theme	total	1389:1393	arg1	= 0.038					1429:1435	pinteraction = 0.038	1416:1435	pinteraction = 0.038	1416:1435	An interaction between HF aetiology and treatment effect was also observed for the secondary outcome of total HF hospitalisations (pinteraction = 0.038).					
35303273	0	0	theme	control	78:84	arg1	analysis					86:93	a quality control analysis	68:93	Quantification of the actual composition of polymeric nanocapsules: a quality control analysis.	0:94	Quantification of the actual composition of polymeric nanocapsules: a quality control analysis.					
35303273	3	1	theme	drugs	628:632	arg1	efficiency					603:612	the association efficiency	587:612	the association efficiency of the loaded drugs instead of their complete final composition	587:676	Usually, reports on the characterization of nanostructures simply indicate the association efficiency of the loaded drugs instead of their complete final composition.					
35303273	4	2	theme	polysialic	1125:1134	arg1	acid					1136:1139	polysialic acid	1125:1139	polysialic acid	1125:1139	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	0	3	theme	quality	70:76	arg1	analysis					86:93	a quality control analysis	68:93	Quantification of the actual composition of polymeric nanocapsules: a quality control analysis.	0:94	Quantification of the actual composition of polymeric nanocapsules: a quality control analysis.					
35303273	7	4	theme	exact	1526:1530	arg1	composition					1532:1542	the exact composition	1522:1542	the exact composition of nanosystems	1522:1557	In our view, the rigorous quantification of the exact composition of nanosystems is essential for the progress of nanotechnology.					
35303273	1	5	theme	polymeric	220:228	arg1	shell					230:234	a polymeric shell	218:234	a polymeric shell	218:234	Nanocapsules (NCs) are drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell.					
35303273	7	6	from	essential	1562:1570	arg1	view					1485:1488	our view	1481:1488	our view	1481:1488	In our view, the rigorous quantification of the exact composition of nanosystems is essential for the progress of nanotechnology.					
35303273	5	7	theme	NCs	1333:1335	arg1	limits					1294:1299	quantification limits	1279:1299	quantification limits	1279:1299	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
35303273	5	7	theme	NCs	1333:1335	arg1	terms					1236:1240	terms	1236:1240	terms of linearity (0.9383 < r2 < 0.9997)	1236:1276	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
35303273	5	7	theme	NCs	1333:1335	arg1	recoveries					1306:1315	recoveries	1306:1315	recoveries	1306:1315	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
35303273	4	8	theme	series	833:838	arg1	components					817:826	all the components	809:826	all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid	809:1199	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	1	9	contain	contain	150:156	arg1	nanosystems					133:143	drug delivery nanosystems	119:143	drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell	119:234	Nanocapsules (NCs) are drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell.					
35303273	1	9	contain	contain	150:156	arg1	Nanocapsules					96:107	Nanocapsules	96:107	Nanocapsules (NCs)	96:113	Nanocapsules (NCs) are drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell.					
35303273	1	9	contain	contain	150:156	arg2	core					166:169	an oily core	158:169	an oily core	158:169	Nanocapsules (NCs) are drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell.					
35303273	2	10	theme	suspending	493:502	arg1	medium					504:509	the NCs' suspending medium	484:509	the NCs' suspending medium	484:509	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	8	11	theme	composition	1732:1742	arg1	influence					1702:1710	the influence	1698:1710	the influence of the nanosystems' composition on their biological performance	1698:1774	This quantitative analysis will allow researchers to draw more accurate conclusions about the influence of the nanosystems' composition on their biological performance.					
35303273	1	12	theme	drug	119:122	arg1	nanosystems					133:143	drug delivery nanosystems	119:143	drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell	119:234	Nanocapsules (NCs) are drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell.					
35303273	1	12	theme	drug	119:122	arg1	Nanocapsules					96:107	Nanocapsules	96:107	Nanocapsules (NCs)	96:113	Nanocapsules (NCs) are drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell.					
35303273	7	13	from	view	1485:1488	arg1	essential					1562:1570	essential	1562:1570	essential	1562:1570	In our view, the rigorous quantification of the exact composition of nanosystems is essential for the progress of nanotechnology.					
35303273	7	13	from	view	1485:1488	arg1	quantification					1504:1517	the rigorous quantification	1491:1517	the rigorous quantification of the exact composition of nanosystems	1491:1557	In our view, the rigorous quantification of the exact composition of nanosystems is essential for the progress of nanotechnology.					
35303273	3	14	theme	complete	651:658	arg1	composition					666:676	their complete final composition	645:676	their complete final composition	645:676	Usually, reports on the characterization of nanostructures simply indicate the association efficiency of the loaded drugs instead of their complete final composition.					
35303273	4	15	theme	spectrometry	745:756	arg1	methodology					763:773	a liquid chromatography (LC) mass spectrometry (MS) methodology	711:773	a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid	711:1199	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	7	16	theme	rigorous	1495:1502	arg1	quantification					1504:1517	the rigorous quantification	1491:1517	the rigorous quantification of the exact composition of nanosystems	1491:1557	In our view, the rigorous quantification of the exact composition of nanosystems is essential for the progress of nanotechnology.					
35303273	7	16	theme	rigorous	1495:1502	arg1	essential					1562:1570	essential	1562:1570	essential	1562:1570	In our view, the rigorous quantification of the exact composition of nanosystems is essential for the progress of nanotechnology.					
35303273	6	17	theme	final	1363:1367	arg1	composition					1369:1379	The final composition	1359:1379	The final composition of the isolated NCs	1359:1399	The final composition of the isolated NCs was found to strongly depend on their composition and preparation technique.					
35303273	3	18	from	reports	521:527	arg1	characterization					536:551	the characterization	532:551	the characterization of nanostructures	532:569	Usually, reports on the characterization of nanostructures simply indicate the association efficiency of the loaded drugs instead of their complete final composition.					
35303273	2	19	theme	remaining	423:431	arg1	amount					433:438	the remaining amount	419:438	the remaining amount	419:438	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	2	19	theme	remaining	423:431	arg1	micelles					472:479	solubilized or forming micelles	449:479	solubilized or forming micelles	449:479	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	4	20	theme	mass	740:743	arg1	MS					759:760	MS	759:760	MS	759:760	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	20	theme	mass	740:743	arg1	spectrometry					745:756	a liquid chromatography (LC) mass spectrometry	711:756	a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid	711:1199	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	21	theme	polyethylene	1163:1174	arg1	acid					1196:1199	polyethylene glycol polyglutamic acid	1163:1199	polyethylene glycol polyglutamic acid	1163:1199	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	6	22	theme	isolated	1388:1395	arg1	NCs					1397:1399	the isolated NCs	1384:1399	the isolated NCs	1384:1399	The final composition of the isolated NCs was found to strongly depend on their composition and preparation technique.					
35303273	1	23	theme	oily	161:164	arg1	core					166:169	an oily core	158:169	an oily core	158:169	Nanocapsules (NCs) are drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell.					
35303273	4	24	theme	glycol	933:938	arg1	succinate					945:953	D-α-tocopherol polyethylene glycol 1000 succinate	905:953	D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides	905:1077	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	2	25	theme	structure	402:410	arg1	fraction					347:354	only a fraction	340:354	only a fraction of each component	340:372	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	2	25	theme	structure	402:410	arg1	part					385:388	finally part	377:388	finally part of the NCs' structure	377:410	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	8	26	theme	accurate	1671:1678	arg1	conclusions					1680:1690	more accurate conclusions	1666:1690	more accurate conclusions about the influence of the nanosystems' composition on their biological performance	1666:1774	This quantitative analysis will allow researchers to draw more accurate conclusions about the influence of the nanosystems' composition on their biological performance.					
35303273	4	27	theme	polyethylene	920:931	arg1	succinate					945:953	D-α-tocopherol polyethylene glycol 1000 succinate	905:953	D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides	905:1077	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	28	theme	polyglutamic	1183:1194	arg1	acid					1196:1199	polyethylene glycol polyglutamic acid	1163:1199	polyethylene glycol polyglutamic acid	1163:1199	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	29	dep	succinate	945:953	arg1	triglycerides					1065:1077	caprylic/capric triglycerides	1049:1077	D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides	905:1077	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	29	dep	succinate	945:953	arg1	hexadecyltrimethylammonium					980:1005	hexadecyltrimethylammonium	980:1005	D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides	905:1077	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	29	dep	succinate	945:953	arg1	lecithin					970:977	lecithin	970:977	D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides	905:1077	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	29	dep	succinate	945:953	arg1	benzethonium					956:967	benzethonium	956:967	D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides	905:1077	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	29	dep	succinate	945:953	arg1	1,2-dioleoyl-3-trimethylammoniumpropane					1008:1046	1,2-dioleoyl-3-trimethylammoniumpropane	1008:1046	D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides	905:1077	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	30	theme	caprylic/capric	1049:1063	arg1	triglycerides					1065:1077	caprylic/capric triglycerides	1049:1077	D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides	905:1077	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	0	31	theme	actual	22:27	arg1	composition					29:39	the actual composition	18:39	the actual composition of polymeric nanocapsules	18:65	Quantification of the actual composition of polymeric nanocapsules: a quality control analysis.					
35303273	7	32	theme	nanotechnology	1592:1605	arg1	progress					1580:1587	the progress	1576:1587	the progress of nanotechnology	1576:1605	In our view, the rigorous quantification of the exact composition of nanosystems is essential for the progress of nanotechnology.					
35303273	4	33	theme	components	817:826	arg1	quantification					791:804	the quantification	787:804	the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid	787:1199	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	34	theme	glycol	1176:1181	arg1	acid					1196:1199	polyethylene glycol polyglutamic acid	1163:1199	polyethylene glycol polyglutamic acid	1163:1199	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	2	35	theme	component	364:372	arg1	fraction					347:354	only a fraction	340:354	only a fraction of each component	340:372	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	2	35	theme	component	364:372	arg1	part					385:388	finally part	377:388	finally part of the NCs' structure	377:410	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	3	36	theme	final	660:664	arg1	composition					666:676	their complete final composition	645:676	their complete final composition	645:676	Usually, reports on the characterization of nanostructures simply indicate the association efficiency of the loaded drugs instead of their complete final composition.					
35303273	2	37	theme	forming	464:470	arg1	amount					433:438	the remaining amount	419:438	the remaining amount	419:438	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	2	37	theme	forming	464:470	arg1	micelles					472:479	solubilized or forming micelles	449:479	solubilized or forming micelles	449:479	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	3	38	theme	nanostructures	556:569	arg1	characterization					536:551	the characterization	532:551	the characterization of nanostructures	532:569	Usually, reports on the characterization of nanostructures simply indicate the association efficiency of the loaded drugs instead of their complete final composition.					
35303273	2	39	theme	components	259:268	arg1	assembling					241:250	The assembling	237:250	The assembling of the components	237:268	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	6	40	theme	preparation	1455:1465	arg1	technique					1467:1475	preparation technique	1455:1475	preparation technique	1455:1475	The final composition of the isolated NCs was found to strongly depend on their composition and preparation technique.					
35303273	4	41	theme	DL-α-tocopherol	888:902	arg1	succinate					945:953	D-α-tocopherol polyethylene glycol 1000 succinate	905:953	D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides	905:1077	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	3	42	theme	composition	666:676	arg1	efficiency					603:612	the association efficiency	587:612	the association efficiency of the loaded drugs instead of their complete final composition	587:676	Usually, reports on the characterization of nanostructures simply indicate the association efficiency of the loaded drugs instead of their complete final composition.					
35303273	1	43	dep	core	166:169	arg1	surrounded					204:213	surrounded	204:213	surrounded by a polymeric shell	204:234	Nanocapsules (NCs) are drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell.					
35303273	1	43	dep	core	166:169	arg1	stabilized					172:181	stabilized	172:181	stabilized by a surfactant	172:197	Nanocapsules (NCs) are drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell.					
35303273	5	44	theme	quantification	1279:1292	arg1	limits					1294:1299	quantification limits	1279:1299	quantification limits	1279:1299	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
35303273	0	45	theme	composition	29:39	arg1	Quantification					0:13	Quantification	0:13	Quantification of the actual composition of polymeric nanocapsules: a quality control analysis.	0:94	Quantification of the actual composition of polymeric nanocapsules: a quality control analysis.					
35303273	2	46	theme	solubilized	449:459	arg1	amount					433:438	the remaining amount	419:438	the remaining amount	419:438	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	2	46	theme	solubilized	449:459	arg1	micelles					472:479	solubilized or forming micelles	449:479	solubilized or forming micelles	449:479	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	4	47	theme	chromatography	720:733	arg1	MS					759:760	MS	759:760	MS	759:760	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	47	theme	chromatography	720:733	arg1	spectrometry					745:756	a liquid chromatography (LC) mass spectrometry	711:756	a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid	711:1199	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	48	theme	hyaluronic	1142:1151	arg1	acid					1153:1156	hyaluronic acid	1142:1156	hyaluronic acid	1142:1156	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	49	theme	NCs	843:845	arg1	series					833:838	a series	831:838	a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid	831:1199	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	0	50	theme	nanocapsules	54:65	arg1	composition					29:39	the actual composition	18:39	the actual composition of polymeric nanocapsules	18:65	Quantification of the actual composition of polymeric nanocapsules: a quality control analysis.					
35303273	4	51	theme	macrogol	1080:1087	arg1	15-hydroxystearate					1089:1106	macrogol 15-hydroxystearate	1080:1106	macrogol 15-hydroxystearate	1080:1106	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	0	52	theme	polymeric	44:52	arg1	nanocapsules					54:65	polymeric nanocapsules	44:65	polymeric nanocapsules	44:65	Quantification of the actual composition of polymeric nanocapsules: a quality control analysis.					
35303273	7	53	theme	nanosystems	1547:1557	arg1	composition					1532:1542	the exact composition	1522:1542	the exact composition of nanosystems	1522:1557	In our view, the rigorous quantification of the exact composition of nanosystems is essential for the progress of nanotechnology.					
35303273	3	54	theme	association	591:601	arg1	efficiency					603:612	the association efficiency	587:612	the association efficiency of the loaded drugs instead of their complete final composition	587:676	Usually, reports on the characterization of nanostructures simply indicate the association efficiency of the loaded drugs instead of their complete final composition.					
35303273	5	55	theme	waste	1342:1346	arg1	fractions					1348:1356	waste fractions	1342:1356	waste fractions	1342:1356	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
35303273	2	56	theme	physicochemical	295:309	arg1	forces					311:316	physical and physicochemical forces	282:316	physical and physicochemical forces	282:316	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	8	57	theme	biological	1753:1762	arg1	performance					1764:1774	their biological performance	1747:1774	their biological performance	1747:1774	This quantitative analysis will allow researchers to draw more accurate conclusions about the influence of the nanosystems' composition on their biological performance.					
35303273	5	58	theme	fractions	1348:1356	arg1	limits					1294:1299	quantification limits	1279:1299	quantification limits	1279:1299	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
35303273	5	58	theme	fractions	1348:1356	arg1	terms					1236:1240	terms	1236:1240	terms of linearity (0.9383 < r2 < 0.9997)	1236:1276	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
35303273	5	58	theme	fractions	1348:1356	arg1	recoveries					1306:1315	recoveries	1306:1315	recoveries	1306:1315	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
35303273	7	59	theme	composition	1532:1542	arg1	quantification					1504:1517	the rigorous quantification	1491:1517	the rigorous quantification of the exact composition of nanosystems	1491:1557	In our view, the rigorous quantification of the exact composition of nanosystems is essential for the progress of nanotechnology.					
35303273	7	59	theme	composition	1532:1542	arg1	essential					1562:1570	essential	1562:1570	essential	1562:1570	In our view, the rigorous quantification of the exact composition of nanosystems is essential for the progress of nanotechnology.					
35303273	8	60	from	influence	1702:1710	arg1	performance					1764:1774	their biological performance	1747:1774	their biological performance	1747:1774	This quantitative analysis will allow researchers to draw more accurate conclusions about the influence of the nanosystems' composition on their biological performance.					
35303273	2	61	theme	physical	282:289	arg1	forces					311:316	physical and physicochemical forces	282:316	physical and physicochemical forces	282:316	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	5	62	theme	linearity	1245:1253	arg1	limits					1294:1299	quantification limits	1279:1299	quantification limits	1279:1299	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
35303273	5	62	theme	linearity	1245:1253	arg1	terms					1236:1240	terms	1236:1240	terms of linearity (0.9383 < r2 < 0.9997)	1236:1276	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
35303273	5	62	theme	linearity	1245:1253	arg1	recoveries					1306:1315	recoveries	1306:1315	recoveries	1306:1315	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
35303273	4	63	theme	different	859:867	arg1	techniques					869:878	different techniques	859:878	different techniques	859:878	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	64	theme	liquid	713:718	arg1	LC					736:737	LC	736:737	LC	736:737	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	4	64	theme	liquid	713:718	arg1	chromatography					720:733	a liquid chromatography	711:733	a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid	711:1199	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	1	65	theme	delivery	124:131	arg1	nanosystems					133:143	drug delivery nanosystems	119:143	drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell	119:234	Nanocapsules (NCs) are drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell.					
35303273	1	65	theme	delivery	124:131	arg1	Nanocapsules					96:107	Nanocapsules	96:107	Nanocapsules (NCs)	96:113	Nanocapsules (NCs) are drug delivery nanosystems that contain an oily core, stabilized by a surfactant, and surrounded by a polymeric shell.					
35303273	8	66	theme	quantitative	1613:1624	arg1	analysis					1626:1633	This quantitative analysis	1608:1633	This quantitative analysis	1608:1633	This quantitative analysis will allow researchers to draw more accurate conclusions about the influence of the nanosystems' composition on their biological performance.					
35303273	2	67	from	micelles	472:479	arg1	medium					504:509	the NCs' suspending medium	484:509	the NCs' suspending medium	484:509	The assembling of the components is based on physical and physicochemical forces, and, hence, usually, only a fraction of each component is finally part of the NCs' structure, while the remaining amount might be solubilized or forming micelles in the NCs' suspending medium.					
35303273	4	68	theme	D-α-tocopherol	905:918	arg1	succinate					945:953	D-α-tocopherol polyethylene glycol 1000 succinate	905:953	D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides	905:1077	In this work, we have developed a liquid chromatography (LC) mass spectrometry (MS) methodology that allows the quantification of all the components of a series of NCs prepared by different techniques, namely DL-α-tocopherol; D-α-tocopherol polyethylene glycol 1000 succinate; benzethonium; lecithin; hexadecyltrimethylammonium; 1,2-dioleoyl-3-trimethylammoniumpropane; caprylic/capric triglycerides; macrogol 15-hydroxystearate; polysorbate 80; polysialic acid; hyaluronic acid; and polyethylene glycol polyglutamic acid.					
35303273	3	69	theme	loaded	621:626	arg1	drugs					628:632	the loaded drugs	617:632	the loaded drugs	617:632	Usually, reports on the characterization of nanostructures simply indicate the association efficiency of the loaded drugs instead of their complete final composition.					
35303273	0	70	dep	Quantification	0:13	arg1	analysis					86:93	a quality control analysis	68:93	Quantification of the actual composition of polymeric nanocapsules: a quality control analysis.	0:94	Quantification of the actual composition of polymeric nanocapsules: a quality control analysis.					
35303273	5	71	theme	isolated	1324:1331	arg1	NCs					1333:1335	the isolated NCs	1320:1335	the isolated NCs'	1320:1336	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
35303273	6	72	theme	NCs	1397:1399	arg1	composition					1369:1379	The final composition	1359:1379	The final composition of the isolated NCs	1359:1399	The final composition of the isolated NCs was found to strongly depend on their composition and preparation technique.					
35303273	5	73	theme	LC-MS	1206:1210	arg1	method					1212:1217	The LC-MS method	1202:1217	The LC-MS method	1202:1217	The LC-MS method was validated in terms of linearity (0.9383 < r2 < 0.9997), quantification limits, and recoveries of the isolated NCs' and waste fractions.					
37070823	3	0	theme	catheter	498:505	arg1	complications					507:519	mechanical catheter complications	487:519	mechanical catheter complications	487:519	We investigated the impact of ELT on mechanical catheter complications during this period.					
37070823	10	1	from	increase	1489:1496	arg1	risk					1501:1504	risk	1501:1504	risk of catheter replacements	1501:1529	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	10	2	theme	ELT	1371:1373	arg1	use					1375:1377	Current ELT use	1363:1377	Current ELT use	1363:1377	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	8	3	used	used	1094:1097	arg2	%					1092:1092	27%	1090:1092	27%	1090:1092	Forty-four percent received ELT for the entirety of the study period, 29% used only heparin locks, and 27% used ELT and heparin locks at different periods.					
37070823	12	4	theme	additional	1840:1849	arg1	procedures					1851:1860	additional procedures	1840:1860	additional procedures	1840:1860	Mechanical complications carry morbidity requiring urgent clinic or emergency department visits and additional procedures.					
37070823	1	5	theme	mechanical	270:279	arg1	complications					290:302	mechanical catheter complications	270:302	mechanical catheter complications	270:302	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	11	6	theme	ELT	1651:1653	arg1	use					1644:1646	use	1644:1646	use	1644:1646	CONCLUSION In the largest pediatric intestinal failure cohort evaluated to date, use of ELT, compared to heparin locks, increased the risk of mechanical catheter complications.					
37070823	7	7	dep	RESULTS	912:918	arg1	consisted					960:968	consisted	960:968	consisted of 122 patients	960:984	RESULTS The pediatric intestinal failure cohort consisted of 122 patients.					
37070823	1	8	theme	central	189:195	arg1	CLABSIs					237:243	CLABSIs	237:243	CLABSIs	237:243	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	1	8	theme	central	189:195	arg1	infections					225:234	central line-associated bloodstream infections	189:234	central line-associated bloodstream infections (CLABSIs)	189:244	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	8	9	theme	study	1043:1047	arg1	period					1049:1054	the study period	1039:1054	the study period	1039:1054	Forty-four percent received ELT for the entirety of the study period, 29% used only heparin locks, and 27% used ELT and heparin locks at different periods.					
37070823	4	10	theme	rehabilitation	636:649	arg1	program					651:657	the Boston Children's Hospital intestinal rehabilitation program	594:657	the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020	594:684	METHODS We performed a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020.					
37070823	0	11	theme	cohort	127:132	arg1	study					134:138	a retrospective cohort study	111:138	a pediatric intestinal failure population: a retrospective cohort study	68:138	Ethanol lock therapy increases mechanical catheter complications in a pediatric intestinal failure population: a retrospective cohort study.					
37070823	9	12	theme	catheter	1203:1210	arg1	complications					1212:1224	mechanical catheter complications	1192:1224	mechanical catheter complications (composite outcome of repairs and replacements)	1192:1272	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	11	13	theme	mechanical	1705:1714	arg1	complications					1725:1737	mechanical catheter complications	1705:1737	mechanical catheter complications	1705:1737	CONCLUSION In the largest pediatric intestinal failure cohort evaluated to date, use of ELT, compared to heparin locks, increased the risk of mechanical catheter complications.					
37070823	1	14	theme	bloodstream	213:223	arg1	CLABSIs					237:243	CLABSIs	237:243	CLABSIs	237:243	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	1	14	theme	bloodstream	213:223	arg1	infections					225:234	central line-associated bloodstream infections	189:234	central line-associated bloodstream infections (CLABSIs)	189:244	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	12	15	theme	emergency	1808:1816	arg1	visits					1829:1834	urgent clinic or emergency department visits	1791:1834	visits	1829:1834	Mechanical complications carry morbidity requiring urgent clinic or emergency department visits and additional procedures.					
37070823	10	16	theme	catheter	1509:1516	arg1	replacements					1518:1529	catheter replacements	1509:1529	catheter replacements	1509:1529	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	11	17	theme	heparin	1668:1674	arg1	locks					1676:1680	heparin locks	1668:1680	heparin locks	1668:1680	CONCLUSION In the largest pediatric intestinal failure cohort evaluated to date, use of ELT, compared to heparin locks, increased the risk of mechanical catheter complications.					
37070823	9	18	theme	rate	1320:1323	arg1	ratio					1325:1329	adjusted incidence rate ratio	1301:1329	adjusted incidence rate ratio [aIRR	1301:1335	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	11	19	theme	failure	1610:1616	arg1	cohort					1618:1623	the largest pediatric intestinal failure cohort	1577:1623	the largest pediatric intestinal failure cohort	1577:1623	CONCLUSION In the largest pediatric intestinal failure cohort evaluated to date, use of ELT, compared to heparin locks, increased the risk of mechanical catheter complications.					
37070823	5	20	theme	venous	721:726	arg1	CVC					738:740	CVC	738:740	CVC	738:740	Pediatric patients with a central venous catheter (CVC) requiring parenteral support for three months were included.					
37070823	5	20	theme	venous	721:726	arg1	catheter					728:735	a central venous catheter	711:735	a central venous catheter (CVC) requiring parenteral support for three months	711:787	Pediatric patients with a central venous catheter (CVC) requiring parenteral support for three months were included.					
37070823	9	21	theme	repairs	1248:1254	arg1	outcome					1237:1243	composite outcome	1227:1243	composite outcome of repairs and replacements	1227:1271	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	1	22	theme	BACKGROUND	141:150	arg1	ELT					174:176	ELT	174:176	ELT	174:176	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	1	22	theme	BACKGROUND	141:150	arg1	therapy					165:171	BACKGROUND Ethanol lock therapy	141:171	BACKGROUND Ethanol lock therapy (ELT)	141:177	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	11	23	from	CONCLUSION	1563:1572	arg1	cohort					1618:1623	the largest pediatric intestinal failure cohort	1577:1623	the largest pediatric intestinal failure cohort	1577:1623	CONCLUSION In the largest pediatric intestinal failure cohort evaluated to date, use of ELT, compared to heparin locks, increased the risk of mechanical catheter complications.					
37070823	11	24	theme	pediatric	1589:1597	arg1	cohort					1618:1623	the largest pediatric intestinal failure cohort	1577:1623	the largest pediatric intestinal failure cohort	1577:1623	CONCLUSION In the largest pediatric intestinal failure cohort evaluated to date, use of ELT, compared to heparin locks, increased the risk of mechanical catheter complications.					
37070823	10	25	dep	aIRR	1439:1442	arg1	%					1453:1453	2.30, 95%	1445:1453	aIRR: 2.30, 95%CI: 1.36-3.89	1439:1466	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	9	26	theme	replacements	1260:1271	arg1	outcome					1237:1243	composite outcome	1227:1243	composite outcome of repairs and replacements	1227:1271	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	1	27	theme	lock	160:163	arg1	ELT					174:176	ELT	174:176	ELT	174:176	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	1	27	theme	lock	160:163	arg1	therapy					165:171	BACKGROUND Ethanol lock therapy	141:171	BACKGROUND Ethanol lock therapy (ELT)	141:177	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	0	28	theme	intestinal	80:89	arg1	population					99:108	a pediatric intestinal failure population	68:108	a pediatric intestinal failure population: a retrospective cohort study	68:138	Ethanol lock therapy increases mechanical catheter complications in a pediatric intestinal failure population: a retrospective cohort study.					
37070823	5	29	theme	parenteral	753:762	arg1	support					764:770	parenteral support	753:770	parenteral support	753:770	Pediatric patients with a central venous catheter (CVC) requiring parenteral support for three months were included.					
37070823	9	30	theme	heparin	1286:1292	arg1	CI					1348:1349	adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31	1301:1359	CI	1348:1349	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	9	30	theme	heparin	1286:1292	arg1	locks					1294:1298	heparin locks	1286:1298	heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31)	1286:1360	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	12	31	theme	urgent	1791:1796	arg1	clinic					1798:1803	urgent clinic or emergency department visits	1791:1834	clinic	1798:1803	Mechanical complications carry morbidity requiring urgent clinic or emergency department visits and additional procedures.					
37070823	8	32	theme	only	1066:1069	arg1	locks					1079:1083	only heparin locks	1066:1083	only heparin locks	1066:1083	Forty-four percent received ELT for the entirety of the study period, 29% used only heparin locks, and 27% used ELT and heparin locks at different periods.					
37070823	0	33	dep	population	99:108	arg1	study					134:138	a retrospective cohort study	111:138	a pediatric intestinal failure population: a retrospective cohort study	68:138	Ethanol lock therapy increases mechanical catheter complications in a pediatric intestinal failure population: a retrospective cohort study.					
37070823	0	34	theme	Ethanol	0:6	arg1	therapy					13:19	Ethanol lock therapy	0:19	Ethanol lock therapy	0:19	Ethanol lock therapy increases mechanical catheter complications in a pediatric intestinal failure population: a retrospective cohort study.					
37070823	9	35	theme	incidence	1310:1318	arg1	ratio					1325:1329	adjusted incidence rate ratio	1301:1329	adjusted incidence rate ratio [aIRR	1301:1335	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	9	36	theme	ratio	1325:1329	arg1	CI					1348:1349	adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31	1301:1359	CI	1348:1349	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	9	36	theme	ratio	1325:1329	arg1	locks					1294:1298	heparin locks	1286:1298	heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31)	1286:1360	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	10	37	theme	catheter	1421:1428	arg1	risk					1413:1416	the risk	1409:1416	the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89)	1409:1467	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	2	38	theme	high-risk	398:406	arg1	patients					408:415	high-risk patients	398:415	high-risk patients switching back to heparin locks	398:447	In recent years, ELT has become unavailable for many patients, often resulting in high-risk patients switching back to heparin locks.					
37070823	0	39	theme	mechanical	31:40	arg1	complications					51:63	mechanical catheter complications	31:63	mechanical catheter complications	31:63	Ethanol lock therapy increases mechanical catheter complications in a pediatric intestinal failure population: a retrospective cohort study.					
37070823	3	40	theme	ELT	480:482	arg1	impact					470:475	the impact	466:475	the impact of ELT on mechanical catheter complications	466:519	We investigated the impact of ELT on mechanical catheter complications during this period.					
37070823	10	41	dep	associated	1383:1392	arg1	aIRR					1532:1535	aIRR	1532:1535	aIRR	1532:1535	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	4	42	dep	METHODS	541:547	arg1	performed					552:560	performed	552:560	performed a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020	552:684	METHODS We performed a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020.					
37070823	6	43	theme	complications	870:882	arg1	outcome					816:822	The primary outcome	804:822	The primary outcome	804:822	The primary outcome was the composite rate of mechanical catheter complications (repairs and replacements).					
37070823	6	43	theme	complications	870:882	arg1	rate					842:845	the composite rate	828:845	the composite rate of mechanical catheter complications (repairs and replacements)	828:909	The primary outcome was the composite rate of mechanical catheter complications (repairs and replacements).					
37070823	3	44	from	impact	470:475	arg1	complications					507:519	mechanical catheter complications	487:519	mechanical catheter complications	487:519	We investigated the impact of ELT on mechanical catheter complications during this period.					
37070823	10	45	dep	aIRR	1532:1535	arg1	1.41					1538:1541	1.41	1538:1541	1.41	1538:1541	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	10	45	dep	aIRR	1532:1535	arg1	%					1546:1546	95%	1544:1546	95%CI: 0.91-2.20	1544:1559	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	8	46	theme	heparin	1107:1113	arg1	locks					1115:1119	heparin locks	1107:1119	heparin locks	1107:1119	Forty-four percent received ELT for the entirety of the study period, 29% used only heparin locks, and 27% used ELT and heparin locks at different periods.					
37070823	6	47	theme	mechanical	850:859	arg1	complications					870:882	mechanical catheter complications	850:882	mechanical catheter complications	850:882	The primary outcome was the composite rate of mechanical catheter complications (repairs and replacements).					
37070823	8	48	theme	Forty-four	987:996	arg1	percent					998:1004	Forty-four percent	987:1004	Forty-four percent	987:1004	Forty-four percent received ELT for the entirety of the study period, 29% used only heparin locks, and 27% used ELT and heparin locks at different periods.					
37070823	4	49	theme	Boston	598:603	arg1	Children					605:612	the Boston Children's	594:614	the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020	594:684	METHODS We performed a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020.					
37070823	9	50	theme	ELT	1150:1152	arg1	use					1154:1156	ELT use	1150:1156	ELT use	1150:1156	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	2	51	theme	many	364:367	arg1	patients					369:376	many patients	364:376	many patients	364:376	In recent years, ELT has become unavailable for many patients, often resulting in high-risk patients switching back to heparin locks.					
37070823	7	52	theme	intestinal	934:943	arg1	cohort					953:958	The pediatric intestinal failure cohort	920:958	The pediatric intestinal failure cohort	920:958	RESULTS The pediatric intestinal failure cohort consisted of 122 patients.					
37070823	5	53	with	patients	697:704	arg1	CVC					738:740	CVC	738:740	CVC	738:740	Pediatric patients with a central venous catheter (CVC) requiring parenteral support for three months were included.					
37070823	5	53	with	patients	697:704	arg1	catheter					728:735	a central venous catheter	711:735	a central venous catheter (CVC) requiring parenteral support for three months	711:787	Pediatric patients with a central venous catheter (CVC) requiring parenteral support for three months were included.					
37070823	9	54	dep	complications	1212:1224	arg1	outcome					1237:1243	composite outcome	1227:1243	composite outcome of repairs and replacements	1227:1271	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	13	55	theme	lock	1892:1895	arg1	solutions					1897:1905	alternative lock solutions	1880:1905	alternative lock solutions	1880:1905	Investigation of alternative lock solutions is warranted.					
37070823	3	56	theme	mechanical	487:496	arg1	complications					507:519	mechanical catheter complications	487:519	mechanical catheter complications	487:519	We investigated the impact of ELT on mechanical catheter complications during this period.					
37070823	4	57	theme	cohort	578:583	arg1	study					585:589	a retrospective cohort study	562:589	a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020	562:684	METHODS We performed a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020.					
37070823	6	58	theme	primary	808:814	arg1	outcome					816:822	The primary outcome	804:822	The primary outcome	804:822	The primary outcome was the composite rate of mechanical catheter complications (repairs and replacements).					
37070823	6	58	theme	primary	808:814	arg1	rate					842:845	the composite rate	828:845	the composite rate of mechanical catheter complications (repairs and replacements)	828:909	The primary outcome was the composite rate of mechanical catheter complications (repairs and replacements).					
37070823	5	59	theme	Pediatric	687:695	arg1	patients					697:704	Pediatric patients	687:704	Pediatric patients with a central venous catheter (CVC) requiring parenteral support for three months	687:787	Pediatric patients with a central venous catheter (CVC) requiring parenteral support for three months were included.					
37070823	6	60	theme	composite	832:840	arg1	outcome					816:822	The primary outcome	804:822	The primary outcome	804:822	The primary outcome was the composite rate of mechanical catheter complications (repairs and replacements).					
37070823	6	60	theme	composite	832:840	arg1	rate					842:845	the composite rate	828:845	the composite rate of mechanical catheter complications (repairs and replacements)	828:909	The primary outcome was the composite rate of mechanical catheter complications (repairs and replacements).					
37070823	10	61	theme	Current	1363:1369	arg1	use					1375:1377	Current ELT use	1363:1377	Current ELT use	1363:1377	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	2	62	theme	recent	319:324	arg1	years					326:330	recent years	319:330	recent years	319:330	In recent years, ELT has become unavailable for many patients, often resulting in high-risk patients switching back to heparin locks.					
37070823	4	63	from	01/01/2018-12/31/2020	664:684	arg1	program					651:657	the Boston Children's Hospital intestinal rehabilitation program	594:657	the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020	594:684	METHODS We performed a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020.					
37070823	4	63	from	01/01/2018-12/31/2020	664:684	arg1	study					585:589	a retrospective cohort study	562:589	a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020	562:684	METHODS We performed a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020.					
37070823	8	64	theme	period	1049:1054	arg1	entirety					1027:1034	the entirety	1023:1034	the entirety of the study period	1023:1054	Forty-four percent received ELT for the entirety of the study period, 29% used only heparin locks, and 27% used ELT and heparin locks at different periods.					
37070823	10	65	theme	significant	1477:1487	arg1	increase					1489:1496	no significant increase	1474:1496	no significant increase in risk of catheter replacements	1474:1529	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	1	66	from	effect	260:265	arg1	complications					290:302	mechanical catheter complications	270:302	mechanical catheter complications	270:302	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	9	67	theme	mechanical	1192:1201	arg1	complications					1212:1224	mechanical catheter complications	1192:1224	mechanical catheter complications (composite outcome of repairs and replacements)	1192:1272	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	1	68	theme	line-associated	197:211	arg1	CLABSIs					237:243	CLABSIs	237:243	CLABSIs	237:243	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	1	68	theme	line-associated	197:211	arg1	infections					225:234	central line-associated bloodstream infections	189:234	central line-associated bloodstream infections (CLABSIs)	189:244	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	6	69	dep	rate	842:845	arg1	repairs					885:891	repairs	885:891	repairs	885:891	The primary outcome was the composite rate of mechanical catheter complications (repairs and replacements).					
37070823	6	69	dep	rate	842:845	arg1	replacements					897:908	replacements	897:908	replacements	897:908	The primary outcome was the composite rate of mechanical catheter complications (repairs and replacements).					
37070823	4	70	theme	intestinal	625:634	arg1	program					651:657	the Boston Children's Hospital intestinal rehabilitation program	594:657	the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020	594:684	METHODS We performed a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020.					
37070823	10	71	theme	replacements	1518:1529	arg1	risk					1501:1504	risk	1501:1504	risk of catheter replacements	1501:1529	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	9	72	theme	complications	1212:1224	arg1	risk					1184:1187	the risk	1180:1187	the risk of mechanical catheter complications (composite outcome of repairs and replacements)	1180:1272	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	4	73	theme	program	651:657	arg1	study					585:589	a retrospective cohort study	562:589	a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020	562:684	METHODS We performed a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020.					
37070823	9	74	theme	composite	1227:1235	arg1	outcome					1237:1243	composite outcome	1227:1243	composite outcome of repairs and replacements	1227:1271	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	12	75	theme	department	1818:1827	arg1	visits					1829:1834	urgent clinic or emergency department visits	1791:1834	visits	1829:1834	Mechanical complications carry morbidity requiring urgent clinic or emergency department visits and additional procedures.					
37070823	11	76	theme	largest	1581:1587	arg1	cohort					1618:1623	the largest pediatric intestinal failure cohort	1577:1623	the largest pediatric intestinal failure cohort	1577:1623	CONCLUSION In the largest pediatric intestinal failure cohort evaluated to date, use of ELT, compared to heparin locks, increased the risk of mechanical catheter complications.					
37070823	5	77	theme	central	713:719	arg1	CVC					738:740	CVC	738:740	CVC	738:740	Pediatric patients with a central venous catheter (CVC) requiring parenteral support for three months were included.					
37070823	5	77	theme	central	713:719	arg1	catheter					728:735	a central venous catheter	711:735	a central venous catheter (CVC) requiring parenteral support for three months	711:787	Pediatric patients with a central venous catheter (CVC) requiring parenteral support for three months were included.					
37070823	0	78	theme	pediatric	70:78	arg1	population					99:108	a pediatric intestinal failure population	68:108	a pediatric intestinal failure population: a retrospective cohort study	68:138	Ethanol lock therapy increases mechanical catheter complications in a pediatric intestinal failure population: a retrospective cohort study.					
37070823	1	79	theme	Ethanol	152:158	arg1	ELT					174:176	ELT	174:176	ELT	174:176	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	1	79	theme	Ethanol	152:158	arg1	therapy					165:171	BACKGROUND Ethanol lock therapy	141:171	BACKGROUND Ethanol lock therapy (ELT)	141:177	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	8	80	used	used	1061:1064	arg2	%					1059:1059	29%	1057:1059	29%	1057:1059	Forty-four percent received ELT for the entirety of the study period, 29% used only heparin locks, and 27% used ELT and heparin locks at different periods.					
37070823	9	81	dep	ratio	1325:1329	arg1	[aIRR					1331:1335	[aIRR	1331:1335	adjusted incidence rate ratio [aIRR	1301:1335	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	11	82	theme	intestinal	1599:1608	arg1	cohort					1618:1623	the largest pediatric intestinal failure cohort	1577:1623	the largest pediatric intestinal failure cohort	1577:1623	CONCLUSION In the largest pediatric intestinal failure cohort evaluated to date, use of ELT, compared to heparin locks, increased the risk of mechanical catheter complications.					
37070823	0	83	theme	failure	91:97	arg1	population					99:108	a pediatric intestinal failure population	68:108	a pediatric intestinal failure population: a retrospective cohort study	68:138	Ethanol lock therapy increases mechanical catheter complications in a pediatric intestinal failure population: a retrospective cohort study.					
37070823	10	84	dep	catheter	1421:1428	arg1	repairs					1430:1436	repairs	1430:1436	repairs	1430:1436	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	10	84	dep	catheter	1421:1428	arg1	aIRR					1439:1442	aIRR	1439:1442	aIRR: 2.30, 95%CI: 1.36-3.89	1439:1466	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	0	85	theme	retrospective	113:125	arg1	study					134:138	a retrospective cohort study	111:138	a pediatric intestinal failure population: a retrospective cohort study	68:138	Ethanol lock therapy increases mechanical catheter complications in a pediatric intestinal failure population: a retrospective cohort study.					
37070823	8	86	theme	heparin	1071:1077	arg1	locks					1079:1083	only heparin locks	1066:1083	only heparin locks	1066:1083	Forty-four percent received ELT for the entirety of the study period, 29% used only heparin locks, and 27% used ELT and heparin locks at different periods.					
37070823	4	87	from	study	585:589	arg1	01/01/2018-12/31/2020					664:684	01/01/2018-12/31/2020	664:684	01/01/2018-12/31/2020	664:684	METHODS We performed a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020.					
37070823	0	88	theme	lock	8:11	arg1	therapy					13:19	Ethanol lock therapy	0:19	Ethanol lock therapy	0:19	Ethanol lock therapy increases mechanical catheter complications in a pediatric intestinal failure population: a retrospective cohort study.					
37070823	9	89	theme	adjusted	1301:1308	arg1	ratio					1325:1329	adjusted incidence rate ratio	1301:1329	adjusted incidence rate ratio [aIRR	1301:1335	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	2	90	theme	heparin	435:441	arg1	locks					443:447	heparin locks	435:447	heparin locks	435:447	In recent years, ELT has become unavailable for many patients, often resulting in high-risk patients switching back to heparin locks.					
37070823	12	91	theme	Mechanical	1740:1749	arg1	complications					1751:1763	Mechanical complications	1740:1763	Mechanical complications	1740:1763	Mechanical complications carry morbidity requiring urgent clinic or emergency department visits and additional procedures.					
37070823	1	92	theme	catheter	281:288	arg1	complications					290:302	mechanical catheter complications	270:302	mechanical catheter complications	270:302	BACKGROUND Ethanol lock therapy (ELT) decreases central line-associated bloodstream infections (CLABSIs); however, the effect on mechanical catheter complications is unclear.					
37070823	4	93	theme	retrospective	564:576	arg1	study					585:589	a retrospective cohort study	562:589	a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020	562:684	METHODS We performed a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020.					
37070823	11	94	theme	catheter	1716:1723	arg1	complications					1725:1737	mechanical catheter complications	1705:1737	mechanical catheter complications	1705:1737	CONCLUSION In the largest pediatric intestinal failure cohort evaluated to date, use of ELT, compared to heparin locks, increased the risk of mechanical catheter complications.					
37070823	0	95	theme	catheter	42:49	arg1	complications					51:63	mechanical catheter complications	31:63	mechanical catheter complications	31:63	Ethanol lock therapy increases mechanical catheter complications in a pediatric intestinal failure population: a retrospective cohort study.					
37070823	13	96	theme	solutions	1897:1905	arg1	Investigation					1863:1875	Investigation	1863:1875	Investigation of alternative lock solutions	1863:1905	Investigation of alternative lock solutions is warranted.					
37070823	7	97	theme	failure	945:951	arg1	cohort					953:958	The pediatric intestinal failure cohort	920:958	The pediatric intestinal failure cohort	920:958	RESULTS The pediatric intestinal failure cohort consisted of 122 patients.					
37070823	6	98	theme	catheter	861:868	arg1	complications					870:882	mechanical catheter complications	850:882	mechanical catheter complications	850:882	The primary outcome was the composite rate of mechanical catheter complications (repairs and replacements).					
37070823	11	99	theme	complications	1725:1737	arg1	risk					1697:1700	the risk	1693:1700	the risk of mechanical catheter complications	1693:1737	CONCLUSION In the largest pediatric intestinal failure cohort evaluated to date, use of ELT, compared to heparin locks, increased the risk of mechanical catheter complications.					
37070823	7	100	theme	pediatric	924:932	arg1	cohort					953:958	The pediatric intestinal failure cohort	920:958	The pediatric intestinal failure cohort	920:958	RESULTS The pediatric intestinal failure cohort consisted of 122 patients.					
37070823	9	101	theme	%	1347:1347	arg1	CI					1348:1349	adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31	1301:1359	CI	1348:1349	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	9	101	theme	%	1347:1347	arg1	locks					1294:1298	heparin locks	1286:1298	heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31)	1286:1360	During ELT use, there was 1.65 times the risk of mechanical catheter complications (composite outcome of repairs and replacements) compared to heparin locks (adjusted incidence rate ratio [aIRR]: 1.65, 95%CI 1.18-2.31).					
37070823	8	102	theme	different	1124:1132	arg1	periods					1134:1140	different periods	1124:1140	different periods	1124:1140	Forty-four percent received ELT for the entirety of the study period, 29% used only heparin locks, and 27% used ELT and heparin locks at different periods.					
37070823	12	103	contain	carry	1765:1769	arg1	complications					1751:1763	Mechanical complications	1740:1763	Mechanical complications	1740:1763	Mechanical complications carry morbidity requiring urgent clinic or emergency department visits and additional procedures.					
37070823	12	103	contain	carry	1765:1769	arg2	morbidity					1771:1779	morbidity	1771:1779	morbidity requiring urgent clinic or emergency department visits and additional procedures	1771:1860	Mechanical complications carry morbidity requiring urgent clinic or emergency department visits and additional procedures.					
37070823	4	104	theme	Hospital	616:623	arg1	program					651:657	the Boston Children's Hospital intestinal rehabilitation program	594:657	the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020	594:684	METHODS We performed a retrospective cohort study of the Boston Children's Hospital intestinal rehabilitation program from 01/01/2018-12/31/2020.					
37070823	13	105	theme	alternative	1880:1890	arg1	solutions					1897:1905	alternative lock solutions	1880:1905	alternative lock solutions	1880:1905	Investigation of alternative lock solutions is warranted.					
37070823	10	106	dep	times	1403:1407	arg1	increase					1489:1496	no significant increase	1474:1496	no significant increase in risk of catheter replacements	1474:1529	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
37070823	10	106	dep	times	1403:1407	arg1	risk					1413:1416	the risk	1409:1416	the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89)	1409:1467	Current ELT use was associated with 2.3 times the risk of catheter repairs (aIRR: 2.30, 95%CI: 1.36-3.89), but no significant increase in risk of catheter replacements (aIRR: 1.41, 95%CI: 0.91-2.20).					
35139764	4	0	theme	composite	924:932	arg1	scaffold					943:950	transfected stem cell composite chitosan scaffold	902:950	the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold	866:950	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	3	1	theme	male	633:636	arg1	rats					658:661	Thirty-two 8-week-old male Sprague-Dawley (SD) rats	611:661	Thirty-two 8-week-old male Sprague-Dawley (SD) rats	611:661	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	0	2	theme	cells	162:166	arg1	analysis					133:140	silent information regulator 7 protein analysis	94:140	silent information regulator 7 protein analysis of mesenchymal stem cells	94:166	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	1	3	theme	Silent	217:222	arg1	SIRT7					249:253	Silent Information Regulator 7 (SIRT7)	217:254	Silent Information Regulator 7 (SIRT7)	217:254	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	9	4	theme	gene	1747:1750	arg1	CKIP-1					1753:1758	gene (CKIP-1)	1747:1759	gene (CKIP-1)	1747:1759	MiR-98-5p targeting and regulating bone formation gene (CKIP-1) could significantly improve the process of osteogenesis in rats.					
35139764	6	5	theme	volume	1398:1403	arg1	fraction					1405:1412	the bone volume fraction	1389:1412	the bone volume fraction	1389:1412	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	1	6	theme	Regulator	236:244	arg1	SIRT7					249:253	Silent Information Regulator 7 (SIRT7)	217:254	Silent Information Regulator 7 (SIRT7)	217:254	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	2	7	theme	bone-related	564:575	arg1	genes					577:581	bone-related genes	564:581	bone-related genes	564:581	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	9	8	theme	bone	1732:1735	arg1	formation					1737:1745	regulating bone formation	1721:1745	regulating bone formation	1721:1745	MiR-98-5p targeting and regulating bone formation gene (CKIP-1) could significantly improve the process of osteogenesis in rats.					
35139764	2	9	theme	human	490:494	arg1	BMMSCs					496:501	human BMMSCs	490:501	human BMMSCs before and after knockout SIRT7	490:533	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	4	10	theme	stem	976:979	arg1	scaffold					1005:1012	the SIRT7 knockout stem cell composite chitosan scaffold	957:1012	the SIRT7 knockout stem cell composite chitosan scaffold	957:1012	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	0	11	theme	information	101:111	arg1	analysis					133:140	silent information regulator 7 protein analysis	94:140	silent information regulator 7 protein analysis of mesenchymal stem cells	94:166	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	6	12	theme	knockout	1369:1376	arg1	group					1378:1382	the medullary mesenchymal stem cell knockout group	1333:1382	the medullary mesenchymal stem cell knockout group	1333:1382	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	4	13	theme	blank	830:834	arg1	group					836:840	the blank group	826:840	the blank group	826:840	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	0	14	from	defect	70:75	arg1	rats					80:83	rats	80:83	rats based on silent information regulator 7 protein analysis of mesenchymal stem cells	80:166	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	4	15	theme	composite	986:994	arg1	scaffold					1005:1012	the SIRT7 knockout stem cell composite chitosan scaffold	957:1012	the SIRT7 knockout stem cell composite chitosan scaffold	957:1012	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	4	16	dep	group	836:840	arg1	addition					814:821	addition	814:821	addition	814:821	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	9	17	theme	osteogenesis	1804:1815	arg1	process					1793:1799	the process	1789:1799	the process of osteogenesis in rats	1789:1823	MiR-98-5p targeting and regulating bone formation gene (CKIP-1) could significantly improve the process of osteogenesis in rats.					
35139764	8	18	theme	Human	1607:1611	arg1	BMMSCs					1613:1618	Human BMMSCs	1607:1618	Human BMMSCs combined with chitosan scaffold	1607:1650	Human BMMSCs combined with chitosan scaffold can accelerate the repair of tibial defects.					
35139764	3	19	theme	information	763:773	arg1	group					794:798	a silence information regulator knockout group 7	753:800	a silence information regulator knockout group 7 (n = 8)	753:808	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	3	19	theme	information	763:773	arg1	n = 8					803:807	n = 8	803:807	n = 8	803:807	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	0	20	theme	mesenchymal	145:155	arg1	cells					162:166	mesenchymal stem cells	145:166	mesenchymal stem cells	145:166	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	1	21	theme	microribonucleic	359:374	arg1	miRNA					382:386	miRNA	382:386	miRNA	382:386	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	1	21	theme	microribonucleic	359:374	arg1	acid					376:379	microribonucleic acid	359:379	microribonucleic acid (miRNA)	359:387	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	2	22	theme	femoral	437:443	arg1	fractures					445:453	femoral fractures	437:453	femoral fractures	437:453	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	0	23	from	rats	80:83	arg1	mechanism					21:29	repair mechanism	14:29	repair mechanism	14:29	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	0	23	from	rats	80:83	arg1	profile					52:58	miRNA expression profile	35:58	miRNA expression profile	35:58	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	3	24	theme	scaffold	716:723	arg1	group					725:729	a chitosan scaffold group	705:729	a chitosan scaffold group	705:729	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	3	24	theme	scaffold	716:723	arg1	group					698:702	a blank group	690:702	a blank group	690:702	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	5	25	theme	small	1085:1089	arg1	Micro-CT					1136:1143	Micro-CT	1136:1143	Micro-CT	1136:1143	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	5	25	theme	small	1085:1089	arg1	tomography					1124:1133	small animal in vivo three-dimensional tomography	1085:1133	small animal in vivo three-dimensional tomography (Micro-CT)	1085:1144	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	8	26	theme	chitosan	1634:1641	arg1	scaffold					1643:1650	chitosan scaffold	1634:1650	chitosan scaffold	1634:1650	Human BMMSCs combined with chitosan scaffold can accelerate the repair of tibial defects.					
35139764	7	27	theme	bone	1572:1575	arg1	structure					1577:1585	bone structure	1572:1585	bone structure	1572:1585	MiR-98-5p can significantly promote the formation of bone molecules and bone structure in rats (P < 0.05).					
35139764	4	28	theme	green	870:874	arg1	GFP					897:899	GFP	897:899	GFP	897:899	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	4	28	theme	green	870:874	arg1	protein					888:894	the green fluorescent protein	866:894	the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold	866:950	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	0	29	theme	defect	70:75	arg1	mechanism					21:29	repair mechanism	14:29	repair mechanism	14:29	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	0	29	theme	defect	70:75	arg1	profile					52:58	miRNA expression profile	35:58	miRNA expression profile	35:58	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	1	30	theme	bone	293:296	arg1	marrow					298:303	bone marrow mesenchymal stem cells (BMMSCs)	293:335	bone marrow mesenchymal stem cells (BMMSCs)	293:335	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	2	31	with	patients	423:430	arg1	fractures					445:453	femoral fractures	437:453	femoral fractures	437:453	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	3	32	theme	knockout	785:792	arg1	group					794:798	a silence information regulator knockout group 7	753:800	a silence information regulator knockout group 7 (n = 8)	753:808	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	3	32	theme	knockout	785:792	arg1	n = 8					803:807	n = 8	803:807	n = 8	803:807	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	2	33	theme	proteins	587:594	arg1	levels					554:559	the expression levels	539:559	the expression levels of bone-related genes and proteins	539:594	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	2	33	theme	proteins	587:594	arg1	activity					478:485	the proliferation activity	460:485	the proliferation activity of human BMMSCs before and after knockout SIRT7	460:533	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	3	34	theme	control	734:740	arg1	group					742:746	a control group	732:746	a control group	732:746	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	3	34	theme	control	734:740	arg1	group					698:702	a blank group	690:702	a blank group	690:702	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	1	35	theme	mesenchymal	305:315	arg1	BMMSCs					329:334	BMMSCs	329:334	BMMSCs	329:334	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	1	35	theme	mesenchymal	305:315	arg1	cells					322:326	mesenchymal stem cells	305:326	bone marrow mesenchymal stem cells (BMMSCs)	293:335	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	4	36	theme	chitosan	847:854	arg1	scaffold					856:863	the chitosan scaffold	843:863	the chitosan scaffold	843:863	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	2	37	theme	genes	577:581	arg1	levels					554:559	the expression levels	539:559	the expression levels of bone-related genes and proteins	539:594	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	2	37	theme	genes	577:581	arg1	activity					478:485	the proliferation activity	460:485	the proliferation activity of human BMMSCs before and after knockout SIRT7	460:533	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	5	38	theme	animal	1091:1096	arg1	Micro-CT					1136:1143	Micro-CT	1136:1143	Micro-CT	1136:1143	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	5	38	theme	animal	1091:1096	arg1	tomography					1124:1133	small animal in vivo three-dimensional tomography	1085:1133	small animal in vivo three-dimensional tomography (Micro-CT)	1085:1144	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	4	39	theme	transfected	902:912	arg1	scaffold					943:950	transfected stem cell composite chitosan scaffold	902:950	the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold	866:950	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	5	40	theme	trabeculae	1220:1229	arg1	fraction					1196:1203	the volume fraction	1185:1203	the volume fraction	1185:1203	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	5	40	theme	trabeculae	1220:1229	arg1	number					1210:1215	the number	1206:1215	the number of trabeculae	1206:1229	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	5	40	theme	trabeculae	1220:1229	arg1	density					1251:1257	the connection density	1236:1257	the connection density	1236:1257	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	6	41	theme	bone	1302:1305	arg1	defect					1307:1312	the bone defect	1298:1312	the bone defect	1298:1312	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	8	42	theme	defects	1688:1694	arg1	repair					1671:1676	the repair	1667:1676	the repair of tibial defects	1667:1694	Human BMMSCs combined with chitosan scaffold can accelerate the repair of tibial defects.					
35139764	4	43	theme	cell	919:922	arg1	scaffold					943:950	transfected stem cell composite chitosan scaffold	902:950	the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold	866:950	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	1	44	dep	marrow	298:303	arg1	BMMSCs					329:334	BMMSCs	329:334	BMMSCs	329:334	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	1	44	dep	marrow	298:303	arg1	cells					322:326	mesenchymal stem cells	305:326	bone marrow mesenchymal stem cells (BMMSCs)	293:335	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	5	45	theme	volume	1189:1194	arg1	fraction					1196:1203	the volume fraction	1185:1203	the volume fraction	1185:1203	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	0	46	theme	repair	14:19	arg1	mechanism					21:29	repair mechanism	14:29	repair mechanism	14:29	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	1	47	theme	SIRT7	249:253	arg1	role					209:212	the role	205:212	the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA)	205:387	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	0	48	theme	expression	41:50	arg1	profile					52:58	miRNA expression profile	35:58	miRNA expression profile	35:58	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	3	49	theme	8-week-old	622:631	arg1	rats					658:661	Thirty-two 8-week-old male Sprague-Dawley (SD) rats	611:661	Thirty-two 8-week-old male Sprague-Dawley (SD) rats	611:661	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	6	50	theme	mesenchymal	1347:1357	arg1	cell					1364:1367	the medullary mesenchymal stem cell	1333:1367	the medullary mesenchymal stem cell knockout group	1333:1382	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	5	51	theme	in	1098:1099	arg1	Micro-CT					1136:1143	Micro-CT	1136:1143	Micro-CT	1136:1143	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	5	51	theme	in	1098:1099	arg1	tomography					1124:1133	small animal in vivo three-dimensional tomography	1085:1133	small animal in vivo three-dimensional tomography (Micro-CT)	1085:1144	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	4	52	theme	knockout	967:974	arg1	scaffold					1005:1012	the SIRT7 knockout stem cell composite chitosan scaffold	957:1012	the SIRT7 knockout stem cell composite chitosan scaffold	957:1012	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	5	53	theme	three-dimensional	1106:1122	arg1	Micro-CT					1136:1143	Micro-CT	1136:1143	Micro-CT	1136:1143	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	5	53	theme	three-dimensional	1106:1122	arg1	tomography					1124:1133	small animal in vivo three-dimensional tomography	1085:1133	small animal in vivo three-dimensional tomography (Micro-CT)	1085:1144	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	4	54	theme	chitosan	934:941	arg1	scaffold					943:950	transfected stem cell composite chitosan scaffold	902:950	the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold	866:950	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	3	55	theme	SD	654:655	arg1	rats					658:661	Thirty-two 8-week-old male Sprague-Dawley (SD) rats	611:661	Thirty-two 8-week-old male Sprague-Dawley (SD) rats	611:661	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	5	56	theme	connection	1240:1249	arg1	density					1251:1257	the connection density	1236:1257	the connection density	1236:1257	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	3	57	theme	Sprague-Dawley	638:651	arg1	rats					658:661	Thirty-two 8-week-old male Sprague-Dawley (SD) rats	611:661	Thirty-two 8-week-old male Sprague-Dawley (SD) rats	611:661	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	4	58	dep	protein	888:894	arg1	scaffold					943:950	transfected stem cell composite chitosan scaffold	902:950	the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold	866:950	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	1	59	theme	Information	224:234	arg1	SIRT7					249:253	Silent Information Regulator 7 (SIRT7)	217:254	Silent Information Regulator 7 (SIRT7)	217:254	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	2	60	theme	knockout	520:527	arg1	SIRT7					529:533	knockout SIRT7	520:533	knockout SIRT7	520:533	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	6	61	theme	bone	1393:1396	arg1	fraction					1405:1412	the bone volume fraction	1389:1412	the bone volume fraction	1389:1412	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	2	62	theme	expression	543:552	arg1	levels					554:559	the expression levels	539:559	the expression levels of bone-related genes and proteins	539:594	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	4	63	theme	cell	981:984	arg1	scaffold					1005:1012	the SIRT7 knockout stem cell composite chitosan scaffold	957:1012	the SIRT7 knockout stem cell composite chitosan scaffold	957:1012	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	3	64	theme	blank	692:696	arg1	group					742:746	a control group	732:746	a control group	732:746	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	3	64	theme	blank	692:696	arg1	group					725:729	a chitosan scaffold group	705:729	a chitosan scaffold group	705:729	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	3	64	theme	blank	692:696	arg1	group					698:702	a blank group	690:702	a blank group	690:702	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	0	65	theme	regulator	113:121	arg1	analysis					133:140	silent information regulator 7 protein analysis	94:140	silent information regulator 7 protein analysis of mesenchymal stem cells	94:166	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	9	66	dep	targeting	1707:1715	arg1	CKIP-1					1753:1758	gene (CKIP-1)	1747:1759	gene (CKIP-1)	1747:1759	MiR-98-5p targeting and regulating bone formation gene (CKIP-1) could significantly improve the process of osteogenesis in rats.					
35139764	4	67	theme	chitosan	996:1003	arg1	scaffold					1005:1012	the SIRT7 knockout stem cell composite chitosan scaffold	957:1012	the SIRT7 knockout stem cell composite chitosan scaffold	957:1012	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	0	68	theme	protein	125:131	arg1	analysis					133:140	silent information regulator 7 protein analysis	94:140	silent information regulator 7 protein analysis of mesenchymal stem cells	94:166	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	7	69	theme	molecules	1558:1566	arg1	formation					1540:1548	the formation	1536:1548	the formation of bone molecules and bone structure in rats (P < 0.05)	1536:1604	MiR-98-5p can significantly promote the formation of bone molecules and bone structure in rats (P < 0.05).					
35139764	6	70	theme	cell	1364:1367	arg1	group					1378:1382	the medullary mesenchymal stem cell knockout group	1333:1382	the medullary mesenchymal stem cell knockout group	1333:1382	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	1	71	theme	study	185:189	arg1	aim					173:175	The aim	169:175	The aim of this study	169:189	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	2	72	theme	proliferation	464:476	arg1	activity					478:485	the proliferation activity	460:485	the proliferation activity of human BMMSCs before and after knockout SIRT7	460:533	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	5	73	dep	in	1098:1099	arg1	vivo					1101:1104	vivo	1101:1104	vivo	1101:1104	The X-rays and small animal in vivo three-dimensional tomography (Micro-CT) were adopted to quantitatively analyze the volume fraction, the number of trabeculae, and the connection density.					
35139764	9	74	from	process	1793:1799	arg1	rats					1820:1823	rats	1820:1823	rats	1820:1823	MiR-98-5p targeting and regulating bone formation gene (CKIP-1) could significantly improve the process of osteogenesis in rats.					
35139764	3	75	theme	chitosan	707:714	arg1	group					725:729	a chitosan scaffold group	705:729	a chitosan scaffold group	705:729	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	3	75	theme	chitosan	707:714	arg1	group					698:702	a blank group	690:702	a blank group	690:702	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	6	76	theme	connection	1440:1449	arg1	density					1451:1457	connection density	1440:1457	connection density	1440:1457	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	0	77	theme	stem	157:160	arg1	cells					162:166	mesenchymal stem cells	145:166	mesenchymal stem cells	145:166	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	6	78	theme	trabeculae	1425:1434	arg1	number					1415:1420	number	1415:1420	number of trabeculae	1415:1434	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	6	78	theme	trabeculae	1425:1434	arg1	fraction					1405:1412	the bone volume fraction	1389:1412	the bone volume fraction	1389:1412	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	6	78	theme	trabeculae	1425:1434	arg1	density					1451:1457	connection density	1440:1457	connection density	1440:1457	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	3	79	theme	regulator	775:783	arg1	group					794:798	a silence information regulator knockout group 7	753:800	a silence information regulator knockout group 7 (n = 8)	753:808	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	3	79	theme	regulator	775:783	arg1	n = 8					803:807	n = 8	803:807	n = 8	803:807	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	2	80	theme	BMMSCs	496:501	arg1	levels					554:559	the expression levels	539:559	the expression levels of bone-related genes and proteins	539:594	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	2	80	theme	BMMSCs	496:501	arg1	activity					478:485	the proliferation activity	460:485	the proliferation activity of human BMMSCs before and after knockout SIRT7	460:533	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	7	81	from	formation	1540:1548	arg1	rats					1590:1593	rats	1590:1593	rats (P < 0.05)	1590:1604	MiR-98-5p can significantly promote the formation of bone molecules and bone structure in rats (P < 0.05).					
35139764	7	81	from	formation	1540:1548	arg1	P					1596:1596	P < 0.05	1596:1603	P < 0.05	1596:1603	MiR-98-5p can significantly promote the formation of bone molecules and bone structure in rats (P < 0.05).					
35139764	4	82	theme	other	1036:1040	arg1	groups					1048:1053	the other three groups	1032:1053	the other three groups	1032:1053	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	0	83	theme	tibial	63:68	arg1	defect					70:75	tibial defect	63:75	tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells	63:166	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	1	84	theme	acid	376:379	arg1	mechanism					280:288	the repair mechanism	269:288	the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs)	269:335	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	1	84	theme	acid	376:379	arg1	expression					345:354	the expression	341:354	the expression of microribonucleic acid (miRNA)	341:387	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	3	85	theme	silence	755:761	arg1	group					794:798	a silence information regulator knockout group 7	753:800	a silence information regulator knockout group 7 (n = 8)	753:808	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	3	85	theme	silence	755:761	arg1	n = 8					803:807	n = 8	803:807	n = 8	803:807	Thirty-two 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a blank group, a chitosan scaffold group, a control group, and a silence information regulator knockout group 7 (n = 8).					
35139764	0	86	from	mechanism	21:29	arg1	rats					80:83	rats	80:83	rats based on silent information regulator 7 protein analysis of mesenchymal stem cells	80:166	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	7	87	theme	structure	1577:1585	arg1	formation					1540:1548	the formation	1536:1548	the formation of bone molecules and bone structure in rats (P < 0.05)	1536:1604	MiR-98-5p can significantly promote the formation of bone molecules and bone structure in rats (P < 0.05).					
35139764	1	88	theme	marrow	298:303	arg1	mechanism					280:288	the repair mechanism	269:288	the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs)	269:335	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	1	88	theme	marrow	298:303	arg1	expression					345:354	the expression	341:354	the expression of microribonucleic acid (miRNA)	341:387	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	0	89	from	profile	52:58	arg1	rats					80:83	rats	80:83	rats based on silent information regulator 7 protein analysis of mesenchymal stem cells	80:166	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	0	90	theme	silent	94:99	arg1	analysis					133:140	silent information regulator 7 protein analysis	94:140	silent information regulator 7 protein analysis of mesenchymal stem cells	94:166	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	1	91	theme	stem	317:320	arg1	BMMSCs					329:334	BMMSCs	329:334	BMMSCs	329:334	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	1	91	theme	stem	317:320	arg1	cells					322:326	mesenchymal stem cells	305:326	bone marrow mesenchymal stem cells (BMMSCs)	293:335	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	6	92	theme	P	1489:1489	arg1	< 0.05					1491:1496	P < 0.05	1489:1496	P < 0.05	1489:1496	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	7	93	theme	bone	1553:1556	arg1	molecules					1558:1566	bone molecules	1553:1566	bone molecules	1553:1566	MiR-98-5p can significantly promote the formation of bone molecules and bone structure in rats (P < 0.05).					
35139764	6	94	dep	increased	1478:1486	arg1	< 0.05					1491:1496	P < 0.05	1489:1496	P < 0.05	1489:1496	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	8	95	theme	tibial	1681:1686	arg1	defects					1688:1694	tibial defects	1681:1694	tibial defects	1681:1694	Human BMMSCs combined with chitosan scaffold can accelerate the repair of tibial defects.					
35139764	4	96	theme	stem	914:917	arg1	scaffold					943:950	transfected stem cell composite chitosan scaffold	902:950	the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold	866:950	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	0	97	theme	miRNA	35:39	arg1	profile					52:58	miRNA expression profile	35:58	miRNA expression profile	35:58	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	4	98	theme	fluorescent	876:886	arg1	GFP					897:899	GFP	897:899	GFP	897:899	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	4	98	theme	fluorescent	876:886	arg1	protein					888:894	the green fluorescent protein	866:894	the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold	866:950	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35139764	6	99	theme	other	1278:1282	arg1	groups					1290:1295	the other three groups	1274:1295	the other three groups	1274:1295	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	2	100	theme	Human	390:394	arg1	BMMSCs					396:401	Human BMMSCs	390:401	Human BMMSCs	390:401	Human BMMSCs were extracted from patients with femoral fractures, and the proliferation activity of human BMMSCs before and after knockout SIRT7 and the expression levels of bone-related genes and proteins were compared.					
35139764	6	101	theme	stem	1359:1362	arg1	cell					1364:1367	the medullary mesenchymal stem cell	1333:1367	the medullary mesenchymal stem cell knockout group	1333:1382	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	1	102	theme	repair	273:278	arg1	mechanism					280:288	the repair mechanism	269:288	the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs)	269:335	The aim of this study was to verify the role of Silent Information Regulator 7 (SIRT7) in improving the repair mechanism of bone marrow mesenchymal stem cells (BMMSCs) and the expression of microribonucleic acid (miRNA).					
35139764	0	103	dep	mechanism	21:29	arg1	the					10:12	the	10:12	the	10:12	Improving the repair mechanism and miRNA expression profile of tibial defect in rats based on silent information regulator 7 protein analysis of mesenchymal stem cells.					
35139764	9	104	theme	MiR-98-5p	1697:1705	arg1	targeting					1707:1715	MiR-98-5p targeting	1697:1715	MiR-98-5p targeting	1697:1715	MiR-98-5p targeting and regulating bone formation gene (CKIP-1) could significantly improve the process of osteogenesis in rats.					
35139764	6	105	theme	medullary	1337:1345	arg1	cell					1364:1367	the medullary mesenchymal stem cell	1333:1367	the medullary mesenchymal stem cell knockout group	1333:1382	Compared with the other three groups, the bone defect was formed more in the medullary mesenchymal stem cell knockout group, and the bone volume fraction, number of trabeculae and connection density were significantly increased (P < 0.05).					
35139764	9	106	theme	regulating	1721:1730	arg1	formation					1737:1745	regulating bone formation	1721:1745	regulating bone formation	1721:1745	MiR-98-5p targeting and regulating bone formation gene (CKIP-1) could significantly improve the process of osteogenesis in rats.					
35139764	4	107	theme	SIRT7	961:965	arg1	scaffold					1005:1012	the SIRT7 knockout stem cell composite chitosan scaffold	957:1012	the SIRT7 knockout stem cell composite chitosan scaffold	957:1012	In addition to the blank group, the chitosan scaffold, the green fluorescent protein (GFP) transfected stem cell composite chitosan scaffold, and the SIRT7 knockout stem cell composite chitosan scaffold were implanted in the other three groups, respectively.					
35314263	0	0	theme	calcium	100:106	arg1	phosphate					128:136	calcium phosphate/strontium phosphate	100:136	calcium phosphate/strontium phosphate adapted to bone regeneration	100:165	Carboxymethyl carrageenan immobilized on 3D-printed polycaprolactone scaffold for the adsorption of calcium phosphate/strontium phosphate adapted to bone regeneration.					
35314263	4	1	theme	phosphate	641:649	arg1	layers					651:656	calcium and strontium phosphate layers	619:656	calcium and strontium phosphate layers	619:656	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	9	2	theme	chain	1597:1601	arg1	reaction					1603:1610	Real Time-Reverse transcription polymerase chain reaction	1554:1610	Real Time-Reverse transcription polymerase chain reaction assays	1554:1617	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	7	3	theme	100	1170:1172	arg1	%					1173:1173	%	1173:1173	%	1173:1173	It was indicated that the highest crystallinity and compressive strength were obtained when 100% CaCl2 was replaced by SrCl2 in the solution (P-C-Sr).					
35314263	9	4	theme	reaction	1603:1610	arg1	assays					1612:1617	Real Time-Reverse transcription polymerase chain reaction assays	1554:1617	Real Time-Reverse transcription polymerase chain reaction assays	1554:1617	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	4	5	from	solutions	832:840	arg1	amounts					804:810	different amounts	794:810	different amounts of strontium in the solutions	794:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	4	5	from	solutions	832:840	arg1	strontium					815:823	strontium	815:823	strontium in the solutions	815:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	2	6	theme	polycaprolactone	382:397	arg1	/kappa-carrageenan					399:416	polycaprolactone /kappa-carrageenan	382:416	polycaprolactone /kappa-carrageenan	382:416	Here, a novel bioactive composite scaffolds from polycaprolactone /kappa-carrageenan were developed for bone regeneration applications.					
35314263	4	7	theme	different	794:802	arg1	amounts					804:810	different amounts	794:810	different amounts of strontium in the solutions	794:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	4	7	theme	different	794:802	arg1	strontium					815:823	strontium	815:823	strontium in the solutions	815:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	0	8	theme	phosphate	128:136	arg1	adsorption					86:95	the adsorption	82:95	the adsorption of calcium phosphate/strontium phosphate adapted to bone regeneration	82:165	Carboxymethyl carrageenan immobilized on 3D-printed polycaprolactone scaffold for the adsorption of calcium phosphate/strontium phosphate adapted to bone regeneration.					
35314263	4	9	theme	organic	587:593	arg1	film					595:598	This organic film	582:598	This organic film	582:598	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	4	10	theme	modified	664:671	arg1	process					691:697	a modified alternate soaking process	662:697	a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions	662:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	0	11	theme	phosphate/strontium	108:126	arg1	phosphate					128:136	calcium phosphate/strontium phosphate	100:136	calcium phosphate/strontium phosphate adapted to bone regeneration	100:165	Carboxymethyl carrageenan immobilized on 3D-printed polycaprolactone scaffold for the adsorption of calcium phosphate/strontium phosphate adapted to bone regeneration.					
35314263	6	12	theme	morphological	965:977	arg1	results					979:985	The morphological results	961:985	The morphological results	961:985	The morphological results demonstrated the highly porous with interconnected pores and uniform pore sizes scaffolds.					
35314263	4	13	theme	calcium	748:754	arg1	ions					756:759	calcium ions	748:759	calcium ions	748:759	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	4	14	theme	strontium	815:823	arg1	amounts					804:810	different amounts	794:810	different amounts of strontium in the solutions	794:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	4	14	theme	strontium	815:823	arg1	strontium					815:823	strontium	815:823	strontium in the solutions	815:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	1	15	theme	bone	298:301	arg1	scaffolds					322:330	the bone tissue engineering scaffolds	294:330	the bone tissue engineering scaffolds	294:330	Three dimensional (3D) substrates based on natural and synthetic polymers enhance the osteogenic and mechanical properties of the bone tissue engineering scaffolds.					
35314263	8	16	theme	Sr	1246:1247	arg1	Incorporation					1229:1241	Incorporation	1229:1241	Incorporation of Sr onto the structure	1229:1266	Incorporation of Sr onto the structure increased the degradation rate of the scaffolds.					
35314263	9	17	theme	transcription	1572:1584	arg1	reaction					1603:1610	Real Time-Reverse transcription polymerase chain reaction	1554:1610	Real Time-Reverse transcription polymerase chain reaction assays	1554:1617	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	4	18	theme	CaCl	702:705	arg1	/SrCl					709:713	CaCl 2 /SrCl 2	702:715	CaCl 2 /SrCl 2	702:715	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	4	19	theme	Na2HPO4	721:727	arg1	solutions					729:737	CaCl 2 /SrCl 2 and Na2HPO4 solutions	702:737	CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions	702:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	9	20	theme	Alkaline	1503:1510	arg1	phosphatase					1512:1522	Alkaline phosphatase	1503:1522	Alkaline phosphatase activity	1503:1531	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	3	21	theme	PCL	472:474	arg1	scaffolds					476:484	3D PCL scaffolds	469:484	3D PCL scaffolds	469:484	3D PCL scaffolds were fabricated by 3D printing method followed by coating with carboxymethyl kappa-carrageenan.					
35314263	2	22	from	/kappa-carrageenan	399:416	arg1	scaffolds					367:375	a novel bioactive composite scaffolds	339:375	a novel bioactive composite scaffolds from polycaprolactone /kappa-carrageenan	339:416	Here, a novel bioactive composite scaffolds from polycaprolactone /kappa-carrageenan were developed for bone regeneration applications.					
35314263	4	23	theme	alternate	673:681	arg1	process					691:697	a modified alternate soaking process	662:697	a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions	662:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	2	24	theme	regeneration	442:453	arg1	applications					455:466	bone regeneration applications	437:466	bone regeneration applications	437:466	Here, a novel bioactive composite scaffolds from polycaprolactone /kappa-carrageenan were developed for bone regeneration applications.					
35314263	7	25	theme	%	1173:1173	arg1	CaCl2					1175:1179	100% CaCl2	1170:1179	100% CaCl2	1170:1179	It was indicated that the highest crystallinity and compressive strength were obtained when 100% CaCl2 was replaced by SrCl2 in the solution (P-C-Sr).					
35314263	4	26	from	amounts	804:810	arg1	solutions					832:840	the solutions	828:840	the solutions	828:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	2	27	theme	novel	341:345	arg1	scaffolds					367:375	a novel bioactive composite scaffolds	339:375	a novel bioactive composite scaffolds from polycaprolactone /kappa-carrageenan	339:416	Here, a novel bioactive composite scaffolds from polycaprolactone /kappa-carrageenan were developed for bone regeneration applications.					
35314263	2	28	theme	bone	437:440	arg1	applications					455:466	bone regeneration applications	437:466	bone regeneration applications	437:466	Here, a novel bioactive composite scaffolds from polycaprolactone /kappa-carrageenan were developed for bone regeneration applications.					
35314263	0	29	theme	Carboxymethyl	0:12	arg1	carrageenan					14:24	Carboxymethyl carrageenan	0:24	Carboxymethyl carrageenan	0:24	Carboxymethyl carrageenan immobilized on 3D-printed polycaprolactone scaffold for the adsorption of calcium phosphate/strontium phosphate adapted to bone regeneration.					
35314263	1	30	theme	tissue	303:308	arg1	scaffolds					322:330	the bone tissue engineering scaffolds	294:330	the bone tissue engineering scaffolds	294:330	Three dimensional (3D) substrates based on natural and synthetic polymers enhance the osteogenic and mechanical properties of the bone tissue engineering scaffolds.					
35314263	4	31	used	used	604:607	arg2	film					595:598	This organic film	582:598	This organic film	582:598	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	5	32	theme	ion	929:931	arg1	effects					908:914	the effects	904:914	the effects of strontium ion on the scaffold properties	904:958	Various characterization techniques were executed to analyze the effects of strontium ion on the scaffold properties.					
35314263	6	33	with	porous	1011:1016	arg1	pores					1038:1042	interconnected pores	1023:1042	interconnected pores	1023:1042	The morphological results demonstrated the highly porous with interconnected pores and uniform pore sizes scaffolds.					
35314263	6	33	with	porous	1011:1016	arg1	sizes					1061:1065	uniform pore sizes	1048:1065	uniform pore sizes	1048:1065	The morphological results demonstrated the highly porous with interconnected pores and uniform pore sizes scaffolds.					
35314263	1	34	theme	natural	211:217	arg1	polymers					233:240	natural and synthetic polymers	211:240	natural and synthetic polymers	211:240	Three dimensional (3D) substrates based on natural and synthetic polymers enhance the osteogenic and mechanical properties of the bone tissue engineering scaffolds.					
35314263	1	35	theme	engineering	310:320	arg1	scaffolds					322:330	the bone tissue engineering scaffolds	294:330	the bone tissue engineering scaffolds	294:330	Three dimensional (3D) substrates based on natural and synthetic polymers enhance the osteogenic and mechanical properties of the bone tissue engineering scaffolds.					
35314263	6	36	theme	uniform	1048:1054	arg1	sizes					1061:1065	uniform pore sizes	1048:1065	uniform pore sizes	1048:1065	The morphological results demonstrated the highly porous with interconnected pores and uniform pore sizes scaffolds.					
35314263	4	37	from	strontium	815:823	arg1	solutions					832:840	the solutions	828:840	the solutions	828:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	8	38	theme	degradation	1282:1292	arg1	rate					1294:1297	the degradation rate	1278:1297	the degradation rate of the scaffolds	1278:1314	Incorporation of Sr onto the structure increased the degradation rate of the scaffolds.					
35314263	9	39	theme	MSCs	1440:1443	arg1	attachment					1408:1417	attachment	1408:1417	attachment	1408:1417	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	9	39	theme	MSCs	1440:1443	arg1	viability					1423:1431	viability	1423:1431	viability	1423:1431	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	1	40	theme	scaffolds	322:330	arg1	properties					280:289	the osteogenic and mechanical properties	250:289	the osteogenic and mechanical properties of the bone tissue engineering scaffolds	250:330	Three dimensional (3D) substrates based on natural and synthetic polymers enhance the osteogenic and mechanical properties of the bone tissue engineering scaffolds.					
35314263	0	41	theme	bone	149:152	arg1	regeneration					154:165	bone regeneration	149:165	bone regeneration	149:165	Carboxymethyl carrageenan immobilized on 3D-printed polycaprolactone scaffold for the adsorption of calcium phosphate/strontium phosphate adapted to bone regeneration.					
35314263	9	42	theme	Mesenchymal	1317:1327	arg1	cells					1334:1338	Mesenchymal stem cells	1317:1338	Mesenchymal stem cells (MSCs) culture on the scaffolds	1317:1370	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	9	42	theme	Mesenchymal	1317:1327	arg1	MSCs					1341:1344	MSCs	1341:1344	MSCs	1341:1344	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	6	43	theme	pore	1056:1059	arg1	sizes					1061:1065	uniform pore sizes	1048:1065	uniform pore sizes	1048:1065	The morphological results demonstrated the highly porous with interconnected pores and uniform pore sizes scaffolds.					
35314263	1	44	theme	synthetic	223:231	arg1	polymers					233:240	natural and synthetic polymers	211:240	natural and synthetic polymers	211:240	Three dimensional (3D) substrates based on natural and synthetic polymers enhance the osteogenic and mechanical properties of the bone tissue engineering scaffolds.					
35314263	0	45	theme	polycaprolactone	52:67	arg1	scaffold					69:76	3D-printed polycaprolactone scaffold	41:76	3D-printed polycaprolactone scaffold for the adsorption of calcium phosphate/strontium phosphate adapted to bone regeneration	41:165	Carboxymethyl carrageenan immobilized on 3D-printed polycaprolactone scaffold for the adsorption of calcium phosphate/strontium phosphate adapted to bone regeneration.					
35314263	4	46	theme	strontium	631:639	arg1	phosphate					641:649	strontium phosphate	631:649	strontium phosphate	631:649	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	9	47	theme	Real	1554:1557	arg1	reaction					1603:1610	Real Time-Reverse transcription polymerase chain reaction	1554:1610	Real Time-Reverse transcription polymerase chain reaction assays	1554:1617	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	5	48	theme	scaffold	940:947	arg1	properties					949:958	the scaffold properties	936:958	the scaffold properties	936:958	Various characterization techniques were executed to analyze the effects of strontium ion on the scaffold properties.					
35314263	3	49	theme	printing	508:515	arg1	method					517:522	3D printing method	505:522	3D printing method followed by coating with carboxymethyl kappa-carrageenan	505:579	3D PCL scaffolds were fabricated by 3D printing method followed by coating with carboxymethyl kappa-carrageenan.					
35314263	0	50	theme	3D-printed	41:50	arg1	scaffold					69:76	3D-printed polycaprolactone scaffold	41:76	3D-printed polycaprolactone scaffold for the adsorption of calcium phosphate/strontium phosphate adapted to bone regeneration	41:165	Carboxymethyl carrageenan immobilized on 3D-printed polycaprolactone scaffold for the adsorption of calcium phosphate/strontium phosphate adapted to bone regeneration.					
35314263	5	51	theme	Various	843:849	arg1	techniques					868:877	Various characterization techniques	843:877	Various characterization techniques	843:877	Various characterization techniques were executed to analyze the effects of strontium ion on the scaffold properties.					
35314263	4	52	theme	/SrCl	709:713	arg1	solutions					729:737	CaCl 2 /SrCl 2 and Na2HPO4 solutions	702:737	CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions	702:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	5	53	theme	characterization	851:866	arg1	techniques					868:877	Various characterization techniques	843:877	Various characterization techniques	843:877	Various characterization techniques were executed to analyze the effects of strontium ion on the scaffold properties.					
35314263	6	54	theme	interconnected	1023:1036	arg1	pores					1038:1042	interconnected pores	1023:1042	interconnected pores	1023:1042	The morphological results demonstrated the highly porous with interconnected pores and uniform pore sizes scaffolds.					
35314263	7	55	theme	compressive	1130:1140	arg1	strength					1142:1149	compressive strength	1130:1149	compressive strength	1130:1149	It was indicated that the highest crystallinity and compressive strength were obtained when 100% CaCl2 was replaced by SrCl2 in the solution (P-C-Sr).					
35314263	4	56	theme	calcium	619:625	arg1	layers					651:656	calcium and strontium phosphate layers	619:656	calcium and strontium phosphate layers	619:656	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	7	57	theme	highest	1104:1110	arg1	crystallinity					1112:1124	crystallinity	1112:1124	crystallinity	1112:1124	It was indicated that the highest crystallinity and compressive strength were obtained when 100% CaCl2 was replaced by SrCl2 in the solution (P-C-Sr).					
35314263	3	58	theme	carboxymethyl	549:561	arg1	kappa-carrageenan					563:579	carboxymethyl kappa-carrageenan	549:579	carboxymethyl kappa-carrageenan	549:579	3D PCL scaffolds were fabricated by 3D printing method followed by coating with carboxymethyl kappa-carrageenan.					
35314263	3	59	theme	3D	469:470	arg1	scaffolds					476:484	3D PCL scaffolds	469:484	3D PCL scaffolds	469:484	3D PCL scaffolds were fabricated by 3D printing method followed by coating with carboxymethyl kappa-carrageenan.					
35314263	9	60	theme	stem	1329:1332	arg1	cells					1334:1338	Mesenchymal stem cells	1317:1338	Mesenchymal stem cells (MSCs) culture on the scaffolds	1317:1370	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	9	60	theme	stem	1329:1332	arg1	MSCs					1341:1344	MSCs	1341:1344	MSCs	1341:1344	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	9	61	theme	phosphatase	1512:1522	arg1	activity					1524:1531	Alkaline phosphatase activity	1503:1531	Alkaline phosphatase activity	1503:1531	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	9	62	theme	polymerase	1586:1595	arg1	reaction					1603:1610	Real Time-Reverse transcription polymerase chain reaction	1554:1610	Real Time-Reverse transcription polymerase chain reaction assays	1554:1617	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	6	63	theme	porous	1011:1016	arg1	scaffolds					1067:1075	the highly porous with interconnected pores and uniform pore sizes scaffolds	1000:1075	the highly porous with interconnected pores and uniform pore sizes scaffolds	1000:1075	The morphological results demonstrated the highly porous with interconnected pores and uniform pore sizes scaffolds.					
35314263	9	64	theme	cells	1334:1338	arg1	culture					1347:1353	Mesenchymal stem cells (MSCs) culture	1317:1353	Mesenchymal stem cells (MSCs) culture on the scaffolds	1317:1370	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	5	65	from	effects	908:914	arg1	properties					949:958	the scaffold properties	936:958	the scaffold properties	936:958	Various characterization techniques were executed to analyze the effects of strontium ion on the scaffold properties.					
35314263	2	66	theme	composite	357:365	arg1	scaffolds					367:375	a novel bioactive composite scaffolds	339:375	a novel bioactive composite scaffolds from polycaprolactone /kappa-carrageenan	339:416	Here, a novel bioactive composite scaffolds from polycaprolactone /kappa-carrageenan were developed for bone regeneration applications.					
35314263	9	67	theme	calcium	1534:1540	arg1	content					1542:1548	calcium content	1534:1548	calcium content	1534:1548	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	4	68	theme	soaking	683:689	arg1	process					691:697	a modified alternate soaking process	662:697	a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions	662:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	2	69	theme	bioactive	347:355	arg1	scaffolds					367:375	a novel bioactive composite scaffolds	339:375	a novel bioactive composite scaffolds from polycaprolactone /kappa-carrageenan	339:416	Here, a novel bioactive composite scaffolds from polycaprolactone /kappa-carrageenan were developed for bone regeneration applications.					
35314263	1	70	theme	osteogenic	254:263	arg1	properties					280:289	the osteogenic and mechanical properties	250:289	the osteogenic and mechanical properties of the bone tissue engineering scaffolds	250:330	Three dimensional (3D) substrates based on natural and synthetic polymers enhance the osteogenic and mechanical properties of the bone tissue engineering scaffolds.					
35314263	9	71	theme	Time-Reverse	1559:1570	arg1	reaction					1603:1610	Real Time-Reverse transcription polymerase chain reaction	1554:1610	Real Time-Reverse transcription polymerase chain reaction assays	1554:1617	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	5	72	theme	strontium	919:927	arg1	ion					929:931	strontium ion	919:931	strontium ion	919:931	Various characterization techniques were executed to analyze the effects of strontium ion on the scaffold properties.					
35314263	9	73	from	culture	1347:1353	arg1	scaffolds					1362:1370	the scaffolds	1358:1370	the scaffolds	1358:1370	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	1	74	theme	dimensional	174:184	arg1	substrates					191:200	Three dimensional (3D) substrates	168:200	Three dimensional (3D) substrates based on natural and synthetic polymers	168:240	Three dimensional (3D) substrates based on natural and synthetic polymers enhance the osteogenic and mechanical properties of the bone tissue engineering scaffolds.					
35314263	1	74	theme	dimensional	174:184	arg1	3D					187:188	3D	187:188	3D	187:188	Three dimensional (3D) substrates based on natural and synthetic polymers enhance the osteogenic and mechanical properties of the bone tissue engineering scaffolds.					
35314263	3	75	theme	3D	505:506	arg1	method					517:522	3D printing method	505:522	3D printing method followed by coating with carboxymethyl kappa-carrageenan	505:579	3D PCL scaffolds were fabricated by 3D printing method followed by coating with carboxymethyl kappa-carrageenan.					
35314263	9	76	theme	osteogenic	1461:1470	arg1	differentiation					1472:1486	osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays	1461:1617	osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays	1461:1617	Mesenchymal stem cells (MSCs) culture on the scaffolds showed that Sr effectively improved attachment and viability of the MSCs and accelerated osteogenic differentiation as revealed by Alkaline phosphatase activity, calcium content and Real Time-Reverse transcription polymerase chain reaction assays.					
35314263	4	77	from	process	691:697	arg1	solutions					729:737	CaCl 2 /SrCl 2 and Na2HPO4 solutions	702:737	CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions	702:840	This organic film was used to create calcium and strontium phosphate layers via a modified alternate soaking process in CaCl 2 /SrCl 2 and Na2HPO4 solutions in which calcium ions were replaced by strontium, with different amounts of strontium in the solutions.					
35314263	8	78	theme	scaffolds	1306:1314	arg1	rate					1294:1297	the degradation rate	1278:1297	the degradation rate of the scaffolds	1278:1314	Incorporation of Sr onto the structure increased the degradation rate of the scaffolds.					
35314263	1	79	theme	mechanical	269:278	arg1	properties					280:289	the osteogenic and mechanical properties	250:289	the osteogenic and mechanical properties of the bone tissue engineering scaffolds	250:330	Three dimensional (3D) substrates based on natural and synthetic polymers enhance the osteogenic and mechanical properties of the bone tissue engineering scaffolds.					
35231534	0	0	theme	cytocompatible	94:107	arg1	nanoplatform					119:130	A cytocompatible and green nanoplatform	92:130	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.	0:173	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.					
35231534	4	1	with	SNP	733:735	arg1	size					762:765	an average particle size	742:765	an average particle size of 13.01 nm and λmax of 421 nm	742:796	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	4	2	theme	TG/RiPE	870:876	arg1	composite					878:886	the gel-like TG/RiPE composite	857:886	the gel-like TG/RiPE composite	857:886	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	6	3	theme	tyrosinase	1110:1119	arg1	functionalities					1148:1162	strong light barrier, tyrosinase inhibitory and antioxidant functionalities	1088:1162	functionalities	1148:1162	Additionally, TG/RiPE-SNG exhibited strong light barrier, tyrosinase inhibitory and antioxidant functionalities.					
35231534	1	4	theme	nanosphere	291:300	arg1	TG/RiPE-SNG					324:334	TG/RiPE-SNG	324:334	TG/RiPE-SNG	324:334	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	1	4	theme	nanosphere	291:300	arg1	nanocomposite					309:321	a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite	239:321	a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities	239:375	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	4	5	theme	gel-like	861:868	arg1	composite					878:886	the gel-like TG/RiPE composite	857:886	the gel-like TG/RiPE composite	857:886	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	8	6	theme	rat	1383:1385	arg1	erythrocytes					1387:1398	rat erythrocytes	1383:1398	rat erythrocytes	1383:1398	Furthermore, TG/RiPE-SNG showed good biocompatibility towards mouse L929 fibroblasts and rat erythrocytes.					
35231534	1	7	theme	hybrid	302:307	arg1	TG/RiPE-SNG					324:334	TG/RiPE-SNG	324:334	TG/RiPE-SNG	324:334	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	1	7	theme	hybrid	302:307	arg1	nanocomposite					309:321	a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite	239:321	a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities	239:375	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	0	8	theme	green	113:117	arg1	nanoplatform					119:130	A cytocompatible and green nanoplatform	92:130	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.	0:173	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.					
35231534	4	9	theme	13.01 nm	770:777	arg1	size					762:765	an average particle size	742:765	an average particle size of 13.01 nm and λmax of 421 nm	742:796	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	1	10	theme	nanocomposite	309:321	arg1	development					224:234	the development	220:234	the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities	220:375	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	9	11	theme	desirable	1658:1666	arg1	potentials					1679:1688	desirable biomedical potentials	1658:1688	desirable biomedical potentials	1658:1688	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	8	12	theme	mouse	1356:1360	arg1	fibroblasts					1367:1377	mouse L929 fibroblasts	1356:1377	mouse L929 fibroblasts	1356:1377	Furthermore, TG/RiPE-SNG showed good biocompatibility towards mouse L929 fibroblasts and rat erythrocytes.					
35231534	7	13	theme	TG/RiPE	1275:1281	arg1	composite					1283:1291	the plain TG/RiPE composite	1265:1291	the plain TG/RiPE composite	1265:1291	TG/RiPE-SNG also exhibited high stability at different pH and was more thermally stable relative to the plain TG/RiPE composite.					
35231534	9	14	theme	natural	1486:1492	arg1	ingredients					1494:1504	only natural ingredients	1481:1504	only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite	1481:1580	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	6	15	theme	antioxidant	1136:1146	arg1	functionalities					1148:1162	strong light barrier, tyrosinase inhibitory and antioxidant functionalities	1088:1162	functionalities	1148:1162	Additionally, TG/RiPE-SNG exhibited strong light barrier, tyrosinase inhibitory and antioxidant functionalities.					
35231534	9	16	theme	nanocomposite	1568:1580	arg1	preparation					1514:1524	the preparation	1510:1524	the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite	1510:1580	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	9	17	theme	gum-based	1529:1537	arg1	nanocomposite					1568:1580	gum-based biopolymer-nanosilver hybrid nanocomposite	1529:1580	gum-based biopolymer-nanosilver hybrid nanocomposite	1529:1580	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	4	18	theme	λmax	783:786	arg1	size					762:765	an average particle size	742:765	an average particle size of 13.01 nm and λmax of 421 nm	742:796	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	4	19	theme	Small	692:696	arg1	SNP					733:735	Small, stable, spherical, well-dispersed SNP	692:735	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm	692:796	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	1	20	theme	one-pot	196:202	arg1	route					210:214	a one-pot green route	194:214	a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities	194:375	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	2	21	theme	Tara	505:508	arg1	gum					510:512	Tara gum	505:512	Tara gum	505:512	The reaction system comprised of AgNO3 as nanosilver precursor, Riceberry phenolic extract as the green in situ reductant, and Tara gum as stabilizing and anchoring agent.					
35231534	9	22	with	nanomaterial	1640:1651	arg1	potentials					1679:1688	desirable biomedical potentials	1658:1688	desirable biomedical potentials	1658:1688	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	7	23	theme	high	1192:1195	arg1	stability					1197:1205	high stability	1192:1205	high stability	1192:1205	TG/RiPE-SNG also exhibited high stability at different pH and was more thermally stable relative to the plain TG/RiPE composite.					
35231534	3	24	theme	UV-vis	598:603	arg1	FESEM					637:641	FESEM	637:641	FESEM	637:641	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	3	24	theme	UV-vis	598:603	arg1	XRD					669:671	XRD	669:671	XRD	669:671	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	3	24	theme	UV-vis	598:603	arg1	FTIR					619:622	FTIR	619:622	FTIR	619:622	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	3	24	theme	UV-vis	598:603	arg1	RAMAN					625:629	RAMAN	625:629	RAMAN	625:629	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	3	24	theme	UV-vis	598:603	arg1	EDX					644:646	EDX	644:646	EDX	644:646	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	3	24	theme	UV-vis	598:603	arg1	potential					658:666	DLS/zeta potential	649:666	DLS/zeta potential	649:666	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	3	24	theme	UV-vis	598:603	arg1	spectroscopy					605:616	UV-vis spectroscopy	598:616	UV-vis spectroscopy	598:616	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	3	24	theme	UV-vis	598:603	arg1	analyses					682:689	TGA analyses	678:689	TGA analyses	678:689	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	3	24	theme	UV-vis	598:603	arg1	TEM					632:634	TEM	632:634	TEM	632:634	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	1	25	theme	green	204:208	arg1	route					210:214	a one-pot green route	194:214	a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities	194:375	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	0	26	with	nanoplatform	119:130	arg1	applications					161:172	multifaceted biological applications	137:172	multifaceted biological applications	137:172	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.					
35231534	4	27	theme	average	745:751	arg1	size					762:765	an average particle size	742:765	an average particle size of 13.01 nm and λmax of 421 nm	742:796	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	2	28	theme	Riceberry	442:450	arg1	extract					461:467	Riceberry phenolic extract	442:467	Riceberry phenolic extract as the green in situ reductant	442:498	The reaction system comprised of AgNO3 as nanosilver precursor, Riceberry phenolic extract as the green in situ reductant, and Tara gum as stabilizing and anchoring agent.					
35231534	0	29	theme	One-pot	0:6	arg1	biofabrication					8:21	One-pot biofabrication	0:21	One-pot biofabrication	0:21	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.					
35231534	9	30	theme	biomedical	1668:1677	arg1	potentials					1679:1688	desirable biomedical potentials	1658:1688	desirable biomedical potentials	1658:1688	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	5	31	dep	gum	1036:1038	arg1	the					1032:1034	the	1032:1034	the	1032:1034	The prepared nanocomposite demonstrated superior antibacterial properties (MIC of 12.5 μg/mL) against S. aureus and S. epidermidis compared to the gum or extract.					
35231534	4	32	dep	Small	692:696	arg1	well-dispersed					718:731	well-dispersed	718:731	well-dispersed	718:731	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	4	32	dep	Small	692:696	arg1	spherical					707:715	spherical	707:715	spherical	707:715	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	4	32	dep	Small	692:696	arg1	stable					699:704	stable	699:704	stable	699:704	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	0	33	theme	biological	150:159	arg1	applications					161:172	multifaceted biological applications	137:172	multifaceted biological applications	137:172	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.					
35231534	5	34	theme	12.5 μg/mL	971:980	arg1	MIC					964:966	MIC	964:966	MIC of 12.5 μg/mL	964:980	The prepared nanocomposite demonstrated superior antibacterial properties (MIC of 12.5 μg/mL) against S. aureus and S. epidermidis compared to the gum or extract.					
35231534	5	34	theme	12.5 μg/mL	971:980	arg1	properties					952:961	superior antibacterial properties	929:961	superior antibacterial properties (MIC of 12.5 μg/mL) against S. aureus and S. epidermidis	929:1018	The prepared nanocomposite demonstrated superior antibacterial properties (MIC of 12.5 μg/mL) against S. aureus and S. epidermidis compared to the gum or extract.					
35231534	6	35	theme	light	1095:1099	arg1	functionalities					1148:1162	strong light barrier, tyrosinase inhibitory and antioxidant functionalities	1088:1162	functionalities	1148:1162	Additionally, TG/RiPE-SNG exhibited strong light barrier, tyrosinase inhibitory and antioxidant functionalities.					
35231534	2	36	theme	in	482:483	arg1	reductant					490:498	the green in situ reductant	472:498	the green in situ reductant	472:498	The reaction system comprised of AgNO3 as nanosilver precursor, Riceberry phenolic extract as the green in situ reductant, and Tara gum as stabilizing and anchoring agent.					
35231534	7	37	theme	different	1210:1218	arg1	pH					1220:1221	different pH	1210:1221	different pH	1210:1221	TG/RiPE-SNG also exhibited high stability at different pH and was more thermally stable relative to the plain TG/RiPE composite.					
35231534	9	38	theme	obtained	1405:1412	arg1	findings					1414:1421	The obtained findings	1401:1421	The obtained findings	1401:1421	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	0	39	theme	multifaceted	137:148	arg1	applications					161:172	multifaceted biological applications	137:172	multifaceted biological applications	137:172	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.					
35231534	4	40	theme	particle	753:760	arg1	size					762:765	an average particle size	742:765	an average particle size of 13.01 nm and λmax of 421 nm	742:796	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	9	41	theme	TG/RiPE-SNP	1623:1633	arg1	nanomaterial					1640:1651	a nanomaterial	1638:1651	a nanomaterial with desirable biomedical potentials	1638:1688	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	9	41	theme	TG/RiPE-SNP	1623:1633	arg1	attributes					1609:1618	the strong attributes	1598:1618	the strong attributes of TG/RiPE-SNP	1598:1633	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	2	42	theme	green	476:480	arg1	reductant					490:498	the green in situ reductant	472:498	the green in situ reductant	472:498	The reaction system comprised of AgNO3 as nanosilver precursor, Riceberry phenolic extract as the green in situ reductant, and Tara gum as stabilizing and anchoring agent.					
35231534	0	43	theme	Tara	47:50	arg1	nanogel					83:89	Tara gum/Riceberry phenolics-silver nanogel	47:89	Tara gum/Riceberry phenolics-silver nanogel	47:89	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.					
35231534	1	44	theme	manifold	342:349	arg1	potentialities					362:375	manifold biological potentialities	342:375	manifold biological potentialities	342:375	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	9	45	theme	biopolymer-nanosilver	1539:1559	arg1	nanocomposite					1568:1580	gum-based biopolymer-nanosilver hybrid nanocomposite	1529:1580	gum-based biopolymer-nanosilver hybrid nanocomposite	1529:1580	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	8	46	theme	good	1326:1329	arg1	biocompatibility					1331:1346	good biocompatibility	1326:1346	good biocompatibility towards mouse L929 fibroblasts and rat erythrocytes	1326:1398	Furthermore, TG/RiPE-SNG showed good biocompatibility towards mouse L929 fibroblasts and rat erythrocytes.					
35231534	6	47	theme	barrier	1101:1107	arg1	functionalities					1148:1162	strong light barrier, tyrosinase inhibitory and antioxidant functionalities	1088:1162	functionalities	1148:1162	Additionally, TG/RiPE-SNG exhibited strong light barrier, tyrosinase inhibitory and antioxidant functionalities.					
35231534	1	48	theme	biological	351:360	arg1	potentialities					362:375	manifold biological potentialities	342:375	manifold biological potentialities	342:375	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	1	49	with	nanocomposite	309:321	arg1	potentialities					362:375	manifold biological potentialities	342:375	manifold biological potentialities	342:375	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	6	50	theme	strong	1088:1093	arg1	functionalities					1148:1162	strong light barrier, tyrosinase inhibitory and antioxidant functionalities	1088:1162	functionalities	1148:1162	Additionally, TG/RiPE-SNG exhibited strong light barrier, tyrosinase inhibitory and antioxidant functionalities.					
35231534	0	51	theme	phenolics-silver	66:81	arg1	nanogel					83:89	Tara gum/Riceberry phenolics-silver nanogel	47:89	Tara gum/Riceberry phenolics-silver nanogel	47:89	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.					
35231534	7	52	theme	plain	1269:1273	arg1	composite					1283:1291	the plain TG/RiPE composite	1265:1291	the plain TG/RiPE composite	1265:1291	TG/RiPE-SNG also exhibited high stability at different pH and was more thermally stable relative to the plain TG/RiPE composite.					
35231534	2	53	dep	in	482:483	arg1	situ					485:488	situ	485:488	situ	485:488	The reaction system comprised of AgNO3 as nanosilver precursor, Riceberry phenolic extract as the green in situ reductant, and Tara gum as stabilizing and anchoring agent.					
35231534	5	54	theme	superior	929:936	arg1	MIC					964:966	MIC	964:966	MIC of 12.5 μg/mL	964:980	The prepared nanocomposite demonstrated superior antibacterial properties (MIC of 12.5 μg/mL) against S. aureus and S. epidermidis compared to the gum or extract.					
35231534	5	54	theme	superior	929:936	arg1	properties					952:961	superior antibacterial properties	929:961	superior antibacterial properties (MIC of 12.5 μg/mL) against S. aureus and S. epidermidis	929:1018	The prepared nanocomposite demonstrated superior antibacterial properties (MIC of 12.5 μg/mL) against S. aureus and S. epidermidis compared to the gum or extract.					
35231534	0	55	theme	gum/Riceberry	52:64	arg1	nanogel					83:89	Tara gum/Riceberry phenolics-silver nanogel	47:89	Tara gum/Riceberry phenolics-silver nanogel	47:89	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.					
35231534	9	56	theme	simple	1434:1439	arg1	approach					1466:1473	a simple, benign, and inexpensive approach	1432:1473	a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials	1432:1688	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	9	57	dep	approach	1466:1473	arg1	underscored					1586:1596	underscored	1586:1596	underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials	1586:1688	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	9	57	dep	approach	1466:1473	arg1	using					1475:1479	using	1475:1479	using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite	1475:1580	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	5	58	theme	antibacterial	938:950	arg1	MIC					964:966	MIC	964:966	MIC of 12.5 μg/mL	964:980	The prepared nanocomposite demonstrated superior antibacterial properties (MIC of 12.5 μg/mL) against S. aureus and S. epidermidis compared to the gum or extract.					
35231534	5	58	theme	antibacterial	938:950	arg1	properties					952:961	superior antibacterial properties	929:961	superior antibacterial properties (MIC of 12.5 μg/mL) against S. aureus and S. epidermidis	929:1018	The prepared nanocomposite demonstrated superior antibacterial properties (MIC of 12.5 μg/mL) against S. aureus and S. epidermidis compared to the gum or extract.					
35231534	4	59	theme	421 nm	791:796	arg1	13.01 nm					770:777	13.01 nm	770:777	13.01 nm	770:777	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	4	59	theme	421 nm	791:796	arg1	λmax					783:786	λmax	783:786	λmax	783:786	Small, stable, spherical, well-dispersed SNP with an average particle size of 13.01 nm and λmax of 421 nm were synthesized in situ, and uniformly distributed within the gel-like TG/RiPE composite.					
35231534	3	60	theme	DLS/zeta	649:656	arg1	spectroscopy					605:616	UV-vis spectroscopy	598:616	UV-vis spectroscopy	598:616	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	3	60	theme	DLS/zeta	649:656	arg1	potential					658:666	DLS/zeta potential	649:666	DLS/zeta potential	649:666	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	8	61	theme	L929	1362:1365	arg1	fibroblasts					1367:1377	mouse L929 fibroblasts	1356:1377	mouse L929 fibroblasts	1356:1377	Furthermore, TG/RiPE-SNG showed good biocompatibility towards mouse L929 fibroblasts and rat erythrocytes.					
35231534	9	62	theme	benign	1442:1447	arg1	approach					1466:1473	a simple, benign, and inexpensive approach	1432:1473	a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials	1432:1688	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	1	63	theme	biocompatible	241:253	arg1	TG/RiPE-SNG					324:334	TG/RiPE-SNG	324:334	TG/RiPE-SNG	324:334	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	1	63	theme	biocompatible	241:253	arg1	nanocomposite					309:321	a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite	239:321	a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities	239:375	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	3	64	theme	TGA	678:680	arg1	spectroscopy					605:616	UV-vis spectroscopy	598:616	UV-vis spectroscopy	598:616	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	3	64	theme	TGA	678:680	arg1	analyses					682:689	TGA analyses	678:689	TGA analyses	678:689	TG/RiPE-SNG was extensively characterized using UV-vis spectroscopy, FTIR, RAMAN, TEM, FESEM, EDX, DLS/zeta potential, XRD, and TGA analyses.					
35231534	2	65	theme	reaction	382:389	arg1	system					391:396	The reaction system	378:396	The reaction system	378:396	The reaction system comprised of AgNO3 as nanosilver precursor, Riceberry phenolic extract as the green in situ reductant, and Tara gum as stabilizing and anchoring agent.					
35231534	1	66	theme	Tara	255:258	arg1	TG/RiPE-SNG					324:334	TG/RiPE-SNG	324:334	TG/RiPE-SNG	324:334	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	1	66	theme	Tara	255:258	arg1	nanocomposite					309:321	a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite	239:321	a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities	239:375	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	9	67	theme	hybrid	1561:1566	arg1	nanocomposite					1568:1580	gum-based biopolymer-nanosilver hybrid nanocomposite	1529:1580	gum-based biopolymer-nanosilver hybrid nanocomposite	1529:1580	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	6	68	theme	inhibitory	1121:1130	arg1	functionalities					1148:1162	strong light barrier, tyrosinase inhibitory and antioxidant functionalities	1088:1162	functionalities	1148:1162	Additionally, TG/RiPE-SNG exhibited strong light barrier, tyrosinase inhibitory and antioxidant functionalities.					
35231534	0	69	dep	biofabrication	8:21	arg1	nanoplatform					119:130	A cytocompatible and green nanoplatform	92:130	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.	0:173	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.					
35231534	9	70	theme	strong	1602:1607	arg1	nanomaterial					1640:1651	a nanomaterial	1638:1651	a nanomaterial with desirable biomedical potentials	1638:1688	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	9	70	theme	strong	1602:1607	arg1	attributes					1609:1618	the strong attributes	1598:1618	the strong attributes of TG/RiPE-SNP	1598:1633	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	5	71	theme	prepared	893:900	arg1	nanocomposite					902:914	The prepared nanocomposite	889:914	The prepared nanocomposite	889:914	The prepared nanocomposite demonstrated superior antibacterial properties (MIC of 12.5 μg/mL) against S. aureus and S. epidermidis compared to the gum or extract.					
35231534	2	72	theme	phenolic	452:459	arg1	extract					461:467	Riceberry phenolic extract	442:467	Riceberry phenolic extract as the green in situ reductant	442:498	The reaction system comprised of AgNO3 as nanosilver precursor, Riceberry phenolic extract as the green in situ reductant, and Tara gum as stabilizing and anchoring agent.					
35231534	1	73	theme	gum-Riceberry	260:272	arg1	TG/RiPE-SNG					324:334	TG/RiPE-SNG	324:334	TG/RiPE-SNG	324:334	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	1	73	theme	gum-Riceberry	260:272	arg1	nanocomposite					309:321	a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite	239:321	a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities	239:375	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	0	74	theme	nanogel	83:89	arg1	characterization					27:42	characterization	27:42	characterization	27:42	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.					
35231534	0	74	theme	nanogel	83:89	arg1	biofabrication					8:21	One-pot biofabrication	0:21	One-pot biofabrication	0:21	One-pot biofabrication and characterization of Tara gum/Riceberry phenolics-silver nanogel: A cytocompatible and green nanoplatform with multifaceted biological applications.					
35231534	9	75	theme	inexpensive	1454:1464	arg1	approach					1466:1473	a simple, benign, and inexpensive approach	1432:1473	a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials	1432:1688	The obtained findings revealed a simple, benign, and inexpensive approach using only natural ingredients for the preparation of gum-based biopolymer-nanosilver hybrid nanocomposite and underscored the strong attributes of TG/RiPE-SNP as a nanomaterial with desirable biomedical potentials.					
35231534	2	76	theme	nanosilver	420:429	arg1	precursor					431:439	nanosilver precursor	420:439	nanosilver precursor	420:439	The reaction system comprised of AgNO3 as nanosilver precursor, Riceberry phenolic extract as the green in situ reductant, and Tara gum as stabilizing and anchoring agent.					
35231534	1	77	theme	phenolics‑silver	274:289	arg1	TG/RiPE-SNG					324:334	TG/RiPE-SNG	324:334	TG/RiPE-SNG	324:334	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
35231534	1	77	theme	phenolics‑silver	274:289	arg1	nanocomposite					309:321	a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite	239:321	a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities	239:375	This work proposed a one-pot green route for the development of a biocompatible Tara gum-Riceberry phenolics‑silver nanosphere hybrid nanocomposite (TG/RiPE-SNG) with manifold biological potentialities.					
36166624	2	0	theme	membranes	346:354	arg1	types					270:274	seven types	264:274	seven types of poly(-caprolactone)-cellulose acetate (PCL-CA) composite nanofiber membranes	264:354	In this study, seven types of poly(-caprolactone)-cellulose acetate (PCL-CA) composite nanofiber membranes were prepared with different proportions of PCL and CA.					
36166624	11	1	theme	PCL-CA	1852:1857	arg1	scaffolds					1867:1875	PCL-CA tubular scaffolds	1852:1875	PCL-CA tubular scaffolds	1852:1875	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	4	2	theme	mm	587:588	arg1	diameter					573:580	a diameter	571:580	a diameter of 4 mm	571:588	A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water.					
36166624	8	3	with	performances	1167:1178	arg1	layers					1226:1231	different layers	1216:1231	different layers	1216:1231	The compression shape memory performances of the PCL-CA tubular scaffold with different layers were also investigated to simulate and analyze the contraction and expansion of tubular scaffolds.					
36166624	7	4	theme	3720	1034:1037	arg1	mmHg					1046:1049	3720 ± 80.5 mmHg	1034:1049	3720 ± 80.5 mmHg	1034:1049	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	6	5	theme	mechanical	782:791	arg1	performance					793:803	The mechanical performance	778:803	The mechanical performance of the PCL-CA tubular scaffold	778:834	The mechanical performance of the PCL-CA tubular scaffold could be improved by increasing the layers.					
36166624	7	6	dep	six-layer	988:996	arg1	mmHg					1046:1049	3720 ± 80.5 mmHg	1034:1049	3720 ± 80.5 mmHg	1034:1049	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	7	6	dep	six-layer	988:996	arg1	mmHg					1011:1014	2380 ± 36.8 mmHg	999:1014	2380 ± 36.8 mmHg	999:1014	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	4	7	with	scaffold	666:673	arg1	water					690:694	50 °C hot water	680:694	50 °C hot water	680:694	A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water.					
36166624	7	8	theme	eight-layer	1021:1031	arg1	scaffolds					1060:1068	six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds	988:1068	six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds	988:1068	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	8	9	theme	shape	1154:1158	arg1	performances					1167:1178	The compression shape memory performances	1138:1178	The compression shape memory performances of the PCL-CA tubular scaffold with different layers	1138:1231	The compression shape memory performances of the PCL-CA tubular scaffold with different layers were also investigated to simulate and analyze the contraction and expansion of tubular scaffolds.					
36166624	10	10	theme	shape	1647:1651	arg1	performance					1660:1670	the compression shape memory performance	1631:1670	the compression shape memory performance of the tubular scaffold	1631:1694	However, the shape fixity rate and shape recovery rate increased, demonstrating that the optimum number of layers can improve the compression shape memory performance of the tubular scaffold.					
36166624	1	11	theme	small-diameter	208:221	arg1	vessels					240:246	small-diameter artificial blood vessels	208:246	small-diameter artificial blood vessels	208:246	Small-diameter artery disease is the most common clinical occurrence, necessitating the development of small-diameter artificial blood vessels.					
36166624	10	12	theme	shape	1518:1522	arg1	rate					1531:1534	the shape fixity rate	1514:1534	the shape fixity rate	1514:1534	However, the shape fixity rate and shape recovery rate increased, demonstrating that the optimum number of layers can improve the compression shape memory performance of the tubular scaffold.					
36166624	4	13	theme	nanofiber	623:631	arg1	membranes					633:641	the prepared nanofiber membranes	610:641	the prepared nanofiber membranes	610:641	A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water.					
36166624	4	14	used	used	594:597	arg2	mandrel					558:564	A smooth stainless-steel mandrel	533:564	A smooth stainless-steel mandrel with a diameter of 4 mm	533:588	A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water.					
36166624	10	15	theme	scaffold	1687:1694	arg1	performance					1660:1670	the compression shape memory performance	1631:1670	the compression shape memory performance of the tubular scaffold	1631:1694	However, the shape fixity rate and shape recovery rate increased, demonstrating that the optimum number of layers can improve the compression shape memory performance of the tubular scaffold.					
36166624	1	16	theme	blood	234:238	arg1	vessels					240:246	small-diameter artificial blood vessels	208:246	small-diameter artificial blood vessels	208:246	Small-diameter artery disease is the most common clinical occurrence, necessitating the development of small-diameter artificial blood vessels.					
36166624	4	17	theme	hot	686:688	arg1	water					690:694	50 °C hot water	680:694	50 °C hot water	680:694	A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water.					
36166624	7	18	theme	six-layer	988:996	arg1	scaffolds					1060:1068	six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds	988:1068	six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds	988:1068	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	11	19	theme	tubular	1859:1865	arg1	scaffolds					1867:1875	PCL-CA tubular scaffolds	1852:1875	PCL-CA tubular scaffolds	1852:1875	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	7	20	theme	human	1104:1108	arg1	vein					1120:1123	the human saphenous vein	1100:1123	the human saphenous vein (2000 mmHg)	1100:1135	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	7	20	theme	human	1104:1108	arg1	mmHg					1131:1134	2000 mmHg	1126:1134	2000 mmHg	1126:1134	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	9	21	theme	tubular	1399:1405	arg1	scaffold					1407:1414	the tubular scaffold	1395:1414	the tubular scaffold in the diameter direction	1395:1440	The experimental results showed that the compression strain of the tubular scaffold in the diameter direction reached 35%, and the ultimate shape recovery rate reached 87%.					
36166624	8	22	theme	tubular	1194:1200	arg1	scaffold					1202:1209	the PCL-CA tubular scaffold	1183:1209	the PCL-CA tubular scaffold	1183:1209	The compression shape memory performances of the PCL-CA tubular scaffold with different layers were also investigated to simulate and analyze the contraction and expansion of tubular scaffolds.					
36166624	9	23	theme	scaffold	1407:1414	arg1	strain					1385:1390	the compression strain	1369:1390	the compression strain of the tubular scaffold in the diameter direction	1369:1440	The experimental results showed that the compression strain of the tubular scaffold in the diameter direction reached 35%, and the ultimate shape recovery rate reached 87%.					
36166624	11	24	theme	engineering	1887:1897	arg1	applicability					1835:1847	the potential applicability	1821:1847	the potential applicability of PCL-CA tubular scaffolds	1821:1875	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	11	24	theme	engineering	1887:1897	arg1	grafts					1899:1904	tissue engineering grafts	1880:1904	tissue engineering grafts	1880:1904	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	7	25	theme	scaffolds	932:940	arg1	BP					913:914	BP	913:914	BP	913:914	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	7	25	theme	scaffolds	932:940	arg1	pressure					903:910	the burst pressure	893:910	the burst pressure (BP) of the tubular scaffolds	893:940	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	9	26	theme	diameter	1423:1430	arg1	direction					1432:1440	the diameter direction	1419:1440	the diameter direction	1419:1440	The experimental results showed that the compression strain of the tubular scaffold in the diameter direction reached 35%, and the ultimate shape recovery rate reached 87%.					
36166624	4	27	theme	50	680:681	arg1	°C					683:684	°C	683:684	°C	683:684	A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water.					
36166624	0	28	theme	Acetate	68:74	arg1	Scaffolds					94:102	Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds	36:102	Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds	36:102	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.					
36166624	8	29	dep	contraction	1284:1294	arg1	the					1280:1282	the	1280:1282	the	1280:1282	The compression shape memory performances of the PCL-CA tubular scaffold with different layers were also investigated to simulate and analyze the contraction and expansion of tubular scaffolds.					
36166624	10	30	theme	optimum	1594:1600	arg1	number					1602:1607	the optimum number	1590:1607	the optimum number of layers	1590:1617	However, the shape fixity rate and shape recovery rate increased, demonstrating that the optimum number of layers can improve the compression shape memory performance of the tubular scaffold.					
36166624	7	31	theme	saphenous	1110:1118	arg1	vein					1120:1123	the human saphenous vein	1100:1123	the human saphenous vein (2000 mmHg)	1100:1135	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	7	31	theme	saphenous	1110:1118	arg1	mmHg					1131:1134	2000 mmHg	1126:1134	2000 mmHg	1126:1134	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	1	32	theme	Small-diameter	105:118	arg1	disease					127:133	Small-diameter artery disease	105:133	Small-diameter artery disease	105:133	Small-diameter artery disease is the most common clinical occurrence, necessitating the development of small-diameter artificial blood vessels.					
36166624	1	32	theme	Small-diameter	105:118	arg1	occurrence					163:172	the most common clinical occurrence	138:172	the most common clinical occurrence	138:172	Small-diameter artery disease is the most common clinical occurrence, necessitating the development of small-diameter artificial blood vessels.					
36166624	0	33	theme	Tubular	86:92	arg1	Scaffolds					94:102	Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds	36:102	Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds	36:102	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.					
36166624	2	34	theme	PCL	400:402	arg1	proportions					385:395	different proportions	375:395	different proportions of PCL and CA	375:409	In this study, seven types of poly(-caprolactone)-cellulose acetate (PCL-CA) composite nanofiber membranes were prepared with different proportions of PCL and CA.					
36166624	7	35	theme	tubular	1052:1058	arg1	scaffolds					1060:1068	six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds	988:1068	six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds	988:1068	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	3	36	dep	in	485:486	arg1	vitro					488:492	vitro	488:492	vitro	488:492	The adhesion and growth of Mc3t3-e1 cells were considered to confirm the in vitro cytocompatibility of PCL-CA membranes.					
36166624	11	37	theme	potential	1825:1833	arg1	grafts					1899:1904	tissue engineering grafts	1880:1904	tissue engineering grafts	1880:1904	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	11	37	theme	potential	1825:1833	arg1	applicability					1835:1847	the potential applicability	1821:1847	the potential applicability of PCL-CA tubular scaffolds	1821:1875	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	10	38	theme	fixity	1524:1529	arg1	rate					1531:1534	the shape fixity rate	1514:1534	the shape fixity rate	1514:1534	However, the shape fixity rate and shape recovery rate increased, demonstrating that the optimum number of layers can improve the compression shape memory performance of the tubular scaffold.					
36166624	0	39	theme	Heat-Stimuli	0:11	arg1	Effect					26:31	Heat-Stimuli Shape Memory Effect	0:31	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.	0:103	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.					
36166624	7	40	theme	burst	897:901	arg1	BP					913:914	BP	913:914	BP	913:914	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	7	40	theme	burst	897:901	arg1	pressure					903:910	the burst pressure	893:910	the burst pressure (BP) of the tubular scaffolds	893:940	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	9	41	theme	ultimate	1463:1470	arg1	rate					1487:1490	the ultimate shape recovery rate	1459:1490	the ultimate shape recovery rate	1459:1490	The experimental results showed that the compression strain of the tubular scaffold in the diameter direction reached 35%, and the ultimate shape recovery rate reached 87%.					
36166624	4	42	theme	stainless-steel	542:556	arg1	mandrel					558:564	A smooth stainless-steel mandrel	533:564	A smooth stainless-steel mandrel with a diameter of 4 mm	533:588	A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water.					
36166624	0	43	theme	Memory	19:24	arg1	Effect					26:31	Heat-Stimuli Shape Memory Effect	0:31	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.	0:103	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.					
36166624	4	44	with	mandrel	558:564	arg1	diameter					573:580	a diameter	571:580	a diameter of 4 mm	571:588	A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water.					
36166624	9	45	theme	recovery	1478:1485	arg1	rate					1487:1490	the ultimate shape recovery rate	1459:1490	the ultimate shape recovery rate	1459:1490	The experimental results showed that the compression strain of the tubular scaffold in the diameter direction reached 35%, and the ultimate shape recovery rate reached 87%.					
36166624	3	46	theme	Mc3t3-e1	439:446	arg1	cells					448:452	Mc3t3-e1 cells	439:452	Mc3t3-e1 cells	439:452	The adhesion and growth of Mc3t3-e1 cells were considered to confirm the in vitro cytocompatibility of PCL-CA membranes.					
36166624	2	47	theme	CA	408:409	arg1	proportions					385:395	different proportions	375:395	different proportions of PCL and CA	375:409	In this study, seven types of poly(-caprolactone)-cellulose acetate (PCL-CA) composite nanofiber membranes were prepared with different proportions of PCL and CA.					
36166624	7	48	theme	scaffolds	1060:1068	arg1	higher					1080:1085	higher	1080:1085	higher	1080:1085	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	7	48	theme	scaffolds	1060:1068	arg1	BPs					981:983	the BPs	977:983	the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds	977:1068	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	5	49	theme	tensile	763:769	arg1	tests					771:775	axial and circumferential tensile tests	737:775	axial and circumferential tensile tests	737:775	The tubular scaffolds were subjected to axial and circumferential tensile tests.					
36166624	9	50	from	strain	1385:1390	arg1	direction					1432:1440	the diameter direction	1419:1440	the diameter direction	1419:1440	The experimental results showed that the compression strain of the tubular scaffold in the diameter direction reached 35%, and the ultimate shape recovery rate reached 87%.					
36166624	2	51	theme	nanofiber	336:344	arg1	membranes					346:354	poly(-caprolactone)-cellulose acetate (PCL-CA) composite nanofiber membranes	279:354	poly(-caprolactone)-cellulose acetate (PCL-CA) composite nanofiber membranes	279:354	In this study, seven types of poly(-caprolactone)-cellulose acetate (PCL-CA) composite nanofiber membranes were prepared with different proportions of PCL and CA.					
36166624	6	52	theme	scaffold	827:834	arg1	performance					793:803	The mechanical performance	778:803	The mechanical performance of the PCL-CA tubular scaffold	778:834	The mechanical performance of the PCL-CA tubular scaffold could be improved by increasing the layers.					
36166624	8	53	theme	scaffolds	1321:1329	arg1	expansion					1300:1308	expansion	1300:1308	expansion	1300:1308	The compression shape memory performances of the PCL-CA tubular scaffold with different layers were also investigated to simulate and analyze the contraction and expansion of tubular scaffolds.					
36166624	8	53	theme	scaffolds	1321:1329	arg1	contraction					1284:1294	contraction	1284:1294	contraction	1284:1294	The compression shape memory performances of the PCL-CA tubular scaffold with different layers were also investigated to simulate and analyze the contraction and expansion of tubular scaffolds.					
36166624	11	54	theme	study	1717:1721	arg1	results					1701:1707	The results	1697:1707	The results	1697:1707	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	11	54	theme	study	1717:1721	arg1	cytocompatibility					1792:1808	cytocompatibility	1792:1808	cytocompatibility	1792:1808	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	11	54	theme	study	1717:1721	arg1	properties					1777:1786	comprehensive morphological and mechanical properties	1734:1786	comprehensive morphological and mechanical properties	1734:1786	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	1	55	theme	vessels	240:246	arg1	development					193:203	the development	189:203	the development of small-diameter artificial blood vessels	189:246	Small-diameter artery disease is the most common clinical occurrence, necessitating the development of small-diameter artificial blood vessels.					
36166624	6	56	theme	PCL-CA	812:817	arg1	scaffold					827:834	the PCL-CA tubular scaffold	808:834	the PCL-CA tubular scaffold	808:834	The mechanical performance of the PCL-CA tubular scaffold could be improved by increasing the layers.					
36166624	4	57	theme	tubular	658:664	arg1	scaffold					666:673	the tubular scaffold	654:673	the tubular scaffold with 50 °C hot water	654:694	A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water.					
36166624	10	58	theme	shape	1540:1544	arg1	rate					1555:1558	shape recovery rate	1540:1558	shape recovery rate	1540:1558	However, the shape fixity rate and shape recovery rate increased, demonstrating that the optimum number of layers can improve the compression shape memory performance of the tubular scaffold.					
36166624	10	59	theme	layers	1612:1617	arg1	number					1602:1607	the optimum number	1590:1607	the optimum number of layers	1590:1617	However, the shape fixity rate and shape recovery rate increased, demonstrating that the optimum number of layers can improve the compression shape memory performance of the tubular scaffold.					
36166624	5	60	theme	axial	737:741	arg1	tests					771:775	axial and circumferential tensile tests	737:775	axial and circumferential tensile tests	737:775	The tubular scaffolds were subjected to axial and circumferential tensile tests.					
36166624	7	61	theme	±	1039:1039	arg1	mmHg					1046:1049	3720 ± 80.5 mmHg	1034:1049	3720 ± 80.5 mmHg	1034:1049	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	11	62	theme	morphological	1748:1760	arg1	properties					1777:1786	comprehensive morphological and mechanical properties	1734:1786	comprehensive morphological and mechanical properties	1734:1786	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	9	63	from	direction	1432:1440	arg1	strain					1385:1390	the compression strain	1369:1390	the compression strain of the tubular scaffold in the diameter direction	1369:1440	The experimental results showed that the compression strain of the tubular scaffold in the diameter direction reached 35%, and the ultimate shape recovery rate reached 87%.					
36166624	10	64	theme	compression	1635:1645	arg1	performance					1660:1670	the compression shape memory performance	1631:1670	the compression shape memory performance of the tubular scaffold	1631:1694	However, the shape fixity rate and shape recovery rate increased, demonstrating that the optimum number of layers can improve the compression shape memory performance of the tubular scaffold.					
36166624	3	65	theme	membranes	522:530	arg1	cytocompatibility					494:510	the in vitro cytocompatibility	481:510	the in vitro cytocompatibility of PCL-CA membranes	481:530	The adhesion and growth of Mc3t3-e1 cells were considered to confirm the in vitro cytocompatibility of PCL-CA membranes.					
36166624	9	66	theme	experimental	1336:1347	arg1	results					1349:1355	The experimental results	1332:1355	The experimental results	1332:1355	The experimental results showed that the compression strain of the tubular scaffold in the diameter direction reached 35%, and the ultimate shape recovery rate reached 87%.					
36166624	10	67	theme	recovery	1546:1553	arg1	rate					1555:1558	shape recovery rate	1540:1558	shape recovery rate	1540:1558	However, the shape fixity rate and shape recovery rate increased, demonstrating that the optimum number of layers can improve the compression shape memory performance of the tubular scaffold.					
36166624	9	68	from	scaffold	1407:1414	arg1	direction					1432:1440	the diameter direction	1419:1440	the diameter direction	1419:1440	The experimental results showed that the compression strain of the tubular scaffold in the diameter direction reached 35%, and the ultimate shape recovery rate reached 87%.					
36166624	7	69	theme	±	1004:1004	arg1	mmHg					1011:1014	2380 ± 36.8 mmHg	999:1014	2380 ± 36.8 mmHg	999:1014	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	10	70	theme	memory	1653:1658	arg1	performance					1660:1670	the compression shape memory performance	1631:1670	the compression shape memory performance of the tubular scaffold	1631:1694	However, the shape fixity rate and shape recovery rate increased, demonstrating that the optimum number of layers can improve the compression shape memory performance of the tubular scaffold.					
36166624	8	71	theme	compression	1142:1152	arg1	performances					1167:1178	The compression shape memory performances	1138:1178	The compression shape memory performances of the PCL-CA tubular scaffold with different layers	1138:1231	The compression shape memory performances of the PCL-CA tubular scaffold with different layers were also investigated to simulate and analyze the contraction and expansion of tubular scaffolds.					
36166624	2	72	theme	composite	326:334	arg1	membranes					346:354	poly(-caprolactone)-cellulose acetate (PCL-CA) composite nanofiber membranes	279:354	poly(-caprolactone)-cellulose acetate (PCL-CA) composite nanofiber membranes	279:354	In this study, seven types of poly(-caprolactone)-cellulose acetate (PCL-CA) composite nanofiber membranes were prepared with different proportions of PCL and CA.					
36166624	11	73	theme	scaffolds	1867:1875	arg1	grafts					1899:1904	tissue engineering grafts	1880:1904	tissue engineering grafts	1880:1904	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	11	73	theme	scaffolds	1867:1875	arg1	applicability					1835:1847	the potential applicability	1821:1847	the potential applicability of PCL-CA tubular scaffolds	1821:1875	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	1	74	theme	artificial	223:232	arg1	vessels					240:246	small-diameter artificial blood vessels	208:246	small-diameter artificial blood vessels	208:246	Small-diameter artery disease is the most common clinical occurrence, necessitating the development of small-diameter artificial blood vessels.					
36166624	8	75	theme	memory	1160:1165	arg1	performances					1167:1178	The compression shape memory performances	1138:1178	The compression shape memory performances of the PCL-CA tubular scaffold with different layers	1138:1231	The compression shape memory performances of the PCL-CA tubular scaffold with different layers were also investigated to simulate and analyze the contraction and expansion of tubular scaffolds.					
36166624	10	76	theme	tubular	1679:1685	arg1	scaffold					1687:1694	the tubular scaffold	1675:1694	the tubular scaffold	1675:1694	However, the shape fixity rate and shape recovery rate increased, demonstrating that the optimum number of layers can improve the compression shape memory performance of the tubular scaffold.					
36166624	1	77	theme	artery	120:125	arg1	disease					127:133	Small-diameter artery disease	105:133	Small-diameter artery disease	105:133	Small-diameter artery disease is the most common clinical occurrence, necessitating the development of small-diameter artificial blood vessels.					
36166624	1	77	theme	artery	120:125	arg1	occurrence					163:172	the most common clinical occurrence	138:172	the most common clinical occurrence	138:172	Small-diameter artery disease is the most common clinical occurrence, necessitating the development of small-diameter artificial blood vessels.					
36166624	4	78	theme	°C	683:684	arg1	water					690:694	50 °C hot water	680:694	50 °C hot water	680:694	A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water.					
36166624	0	79	theme	Poly	36:39	arg1	Scaffolds					94:102	Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds	36:102	Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds	36:102	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.					
36166624	8	80	theme	PCL-CA	1187:1192	arg1	scaffold					1202:1209	the PCL-CA tubular scaffold	1183:1209	the PCL-CA tubular scaffold	1183:1209	The compression shape memory performances of the PCL-CA tubular scaffold with different layers were also investigated to simulate and analyze the contraction and expansion of tubular scaffolds.					
36166624	0	81	theme	-Cellulose	57:66	arg1	Scaffolds					94:102	Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds	36:102	Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds	36:102	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.					
36166624	11	82	theme	tissue	1880:1885	arg1	applicability					1835:1847	the potential applicability	1821:1847	the potential applicability of PCL-CA tubular scaffolds	1821:1875	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	11	82	theme	tissue	1880:1885	arg1	grafts					1899:1904	tissue engineering grafts	1880:1904	tissue engineering grafts	1880:1904	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	8	83	theme	scaffold	1202:1209	arg1	performances					1167:1178	The compression shape memory performances	1138:1178	The compression shape memory performances of the PCL-CA tubular scaffold with different layers	1138:1231	The compression shape memory performances of the PCL-CA tubular scaffold with different layers were also investigated to simulate and analyze the contraction and expansion of tubular scaffolds.					
36166624	5	84	theme	tubular	701:707	arg1	scaffolds					709:717	The tubular scaffolds	697:717	The tubular scaffolds	697:717	The tubular scaffolds were subjected to axial and circumferential tensile tests.					
36166624	0	85	theme	Composite	76:84	arg1	Scaffolds					94:102	Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds	36:102	Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds	36:102	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.					
36166624	1	86	theme	clinical	154:161	arg1	disease					127:133	Small-diameter artery disease	105:133	Small-diameter artery disease	105:133	Small-diameter artery disease is the most common clinical occurrence, necessitating the development of small-diameter artificial blood vessels.					
36166624	1	86	theme	clinical	154:161	arg1	occurrence					163:172	the most common clinical occurrence	138:172	the most common clinical occurrence	138:172	Small-diameter artery disease is the most common clinical occurrence, necessitating the development of small-diameter artificial blood vessels.					
36166624	8	87	theme	different	1216:1224	arg1	layers					1226:1231	different layers	1216:1231	different layers	1216:1231	The compression shape memory performances of the PCL-CA tubular scaffold with different layers were also investigated to simulate and analyze the contraction and expansion of tubular scaffolds.					
36166624	0	88	theme	Scaffolds	94:102	arg1	Effect					26:31	Heat-Stimuli Shape Memory Effect	0:31	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.	0:103	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.					
36166624	7	89	theme	tubular	924:930	arg1	scaffolds					932:940	the tubular scaffolds	920:940	the tubular scaffolds	920:940	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	9	90	theme	compression	1373:1383	arg1	strain					1385:1390	the compression strain	1369:1390	the compression strain of the tubular scaffold in the diameter direction	1369:1440	The experimental results showed that the compression strain of the tubular scaffold in the diameter direction reached 35%, and the ultimate shape recovery rate reached 87%.					
36166624	0	91	theme	Shape	13:17	arg1	Effect					26:31	Heat-Stimuli Shape Memory Effect	0:31	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.	0:103	Heat-Stimuli Shape Memory Effect of Poly (ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds.					
36166624	2	92	theme	different	375:383	arg1	proportions					385:395	different proportions	375:395	different proportions of PCL and CA	375:409	In this study, seven types of poly(-caprolactone)-cellulose acetate (PCL-CA) composite nanofiber membranes were prepared with different proportions of PCL and CA.					
36166624	9	93	theme	shape	1472:1476	arg1	rate					1487:1490	the ultimate shape recovery rate	1459:1490	the ultimate shape recovery rate	1459:1490	The experimental results showed that the compression strain of the tubular scaffold in the diameter direction reached 35%, and the ultimate shape recovery rate reached 87%.					
36166624	11	94	theme	mechanical	1766:1775	arg1	properties					1777:1786	comprehensive morphological and mechanical properties	1734:1786	comprehensive morphological and mechanical properties	1734:1786	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	5	95	theme	circumferential	747:761	arg1	tests					771:775	axial and circumferential tensile tests	737:775	axial and circumferential tensile tests	737:775	The tubular scaffolds were subjected to axial and circumferential tensile tests.					
36166624	3	96	dep	adhesion	416:423	arg1	The					412:414	The	412:414	The	412:414	The adhesion and growth of Mc3t3-e1 cells were considered to confirm the in vitro cytocompatibility of PCL-CA membranes.					
36166624	3	97	theme	cells	448:452	arg1	growth					429:434	growth	429:434	growth	429:434	The adhesion and growth of Mc3t3-e1 cells were considered to confirm the in vitro cytocompatibility of PCL-CA membranes.					
36166624	3	97	theme	cells	448:452	arg1	adhesion					416:423	adhesion	416:423	adhesion	416:423	The adhesion and growth of Mc3t3-e1 cells were considered to confirm the in vitro cytocompatibility of PCL-CA membranes.					
36166624	7	98	theme	2380	999:1002	arg1	mmHg					1011:1014	2380 ± 36.8 mmHg	999:1014	2380 ± 36.8 mmHg	999:1014	In addition, the burst pressure (BP) of the tubular scaffolds was increased with the layers, and the BPs of six-layer (2380 ± 36.8 mmHg) and eight-layer (3720 ± 80.5 mmHg) tubular scaffolds were much higher than that of the human saphenous vein (2000 mmHg).					
36166624	4	99	theme	smooth	535:540	arg1	mandrel					558:564	A smooth stainless-steel mandrel	533:564	A smooth stainless-steel mandrel with a diameter of 4 mm	533:588	A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water.					
36166624	1	100	theme	common	147:152	arg1	disease					127:133	Small-diameter artery disease	105:133	Small-diameter artery disease	105:133	Small-diameter artery disease is the most common clinical occurrence, necessitating the development of small-diameter artificial blood vessels.					
36166624	1	100	theme	common	147:152	arg1	occurrence					163:172	the most common clinical occurrence	138:172	the most common clinical occurrence	138:172	Small-diameter artery disease is the most common clinical occurrence, necessitating the development of small-diameter artificial blood vessels.					
36166624	3	101	theme	in	485:486	arg1	cytocompatibility					494:510	the in vitro cytocompatibility	481:510	the in vitro cytocompatibility of PCL-CA membranes	481:530	The adhesion and growth of Mc3t3-e1 cells were considered to confirm the in vitro cytocompatibility of PCL-CA membranes.					
36166624	11	102	theme	comprehensive	1734:1746	arg1	properties					1777:1786	comprehensive morphological and mechanical properties	1734:1786	comprehensive morphological and mechanical properties	1734:1786	The results of this study, including comprehensive morphological and mechanical properties and cytocompatibility, indicated the potential applicability of PCL-CA tubular scaffolds as tissue engineering grafts.					
36166624	6	103	theme	tubular	819:825	arg1	scaffold					827:834	the PCL-CA tubular scaffold	808:834	the PCL-CA tubular scaffold	808:834	The mechanical performance of the PCL-CA tubular scaffold could be improved by increasing the layers.					
36166624	8	104	theme	tubular	1313:1319	arg1	scaffolds					1321:1329	tubular scaffolds	1313:1329	tubular scaffolds	1313:1329	The compression shape memory performances of the PCL-CA tubular scaffold with different layers were also investigated to simulate and analyze the contraction and expansion of tubular scaffolds.					
36166624	4	105	theme	prepared	614:621	arg1	membranes					633:641	the prepared nanofiber membranes	610:641	the prepared nanofiber membranes	610:641	A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water.					
36166624	3	106	theme	PCL-CA	515:520	arg1	membranes					522:530	PCL-CA membranes	515:530	PCL-CA membranes	515:530	The adhesion and growth of Mc3t3-e1 cells were considered to confirm the in vitro cytocompatibility of PCL-CA membranes.					
36327083	9	0	theme	Langmuir	1290:1297	arg1	isotherm					1299:1306	Langmuir isotherm	1290:1306	Langmuir isotherm	1290:1306	The adsorption isotherm data were successfully fitted to Langmuir isotherm for AWC and CAW beads, while WAC beads followed the Freundlich isotherm.					
36327083	2	1	theme	X-ray	684:688	arg1	spectroscopy					690:701	energy-dispersive X-ray spectroscopy	666:701	energy-dispersive X-ray spectroscopy	666:701	The beads were characterized by a scanning electron microscope, Fourier transform infrared spectrum spectrophotometer, and energy-dispersive X-ray spectroscopy.					
36327083	10	2	theme	CAW	1428:1430	arg1	beads					1432:1436	CAW beads	1428:1436	CAW beads	1428:1436	The highest adsorption was attained at pH 5 on CAW beads and pH 7 on AWC and WAC beads.					
36327083	11	3	theme	equilibrium	1498:1508	arg1	studies					1510:1516	equilibrium studies	1498:1516	equilibrium studies	1498:1516	The optimal contact time for equilibrium studies was 120 min for all types of beads.					
36327083	14	4	used	used	1818:1821	arg2	beads					1805:1809	prepared combination beads	1784:1809	prepared combination beads	1784:1809	The results indicate that prepared combination beads can be used to remove antibiotics from wastewater.					
36327083	10	5	theme	AWC	1450:1452	arg1	beads					1462:1466	AWC and WAC beads	1450:1466	AWC and WAC beads	1450:1466	The highest adsorption was attained at pH 5 on CAW beads and pH 7 on AWC and WAC beads.					
36327083	2	6	theme	scanning	577:584	arg1	microscope					595:604	a scanning electron microscope	575:604	a scanning electron microscope	575:604	The beads were characterized by a scanning electron microscope, Fourier transform infrared spectrum spectrophotometer, and energy-dispersive X-ray spectroscopy.					
36327083	1	7	theme	antibiotics	485:495	arg1	removal					474:480	the removal	470:480	the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater	470:540	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	8	theme	almond	299:304	arg1	shell					306:310	almond shell	299:310	chitosan:almond shell:walnut 2:1:1 (CAW)	290:329	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	12	9	theme	isotherm	1569:1576	arg1	data					1578:1581	The adsorption isotherm data	1554:1581	The adsorption isotherm data in AWC beads	1554:1594	The adsorption isotherm data in AWC beads fit well with the Langmuir model and Freundlich adsorption for CAW and WAC beads.					
36327083	0	10	theme	aqueous	112:118	arg1	solutions					120:128	aqueous solutions	112:128	aqueous solutions	112:128	Chitosan beads coated with almond and walnut shells for the adsorption of gatifloxacin antibiotic compound from aqueous solutions.					
36327083	0	11	from	adsorption	60:69	arg1	solutions					120:128	aqueous solutions	112:128	aqueous solutions	112:128	Chitosan beads coated with almond and walnut shells for the adsorption of gatifloxacin antibiotic compound from aqueous solutions.					
36327083	10	12	theme	WAC	1458:1460	arg1	beads					1462:1466	AWC and WAC beads	1450:1466	AWC and WAC beads	1450:1466	The highest adsorption was attained at pH 5 on CAW beads and pH 7 on AWC and WAC beads.					
36327083	5	13	theme	initial	933:939	arg1	's					918:919	the adsorbent's dosage, pH, initial concentration, and contact time	905:971	the adsorbent's dosage, pH, initial concentration, and contact time	905:971	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	5	13	theme	initial	933:939	arg1	concentration					941:953	initial concentration	933:953	initial concentration	933:953	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	1	14	theme	synthetic	521:529	arg1	wastewater					531:540	synthetic wastewater	521:540	synthetic wastewater	521:540	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	5	15	theme	adsorbent	909:917	arg1	dosage					921:926	dosage	921:926	dosage	921:926	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	5	15	theme	adsorbent	909:917	arg1	time					968:971	contact time	960:971	contact time	960:971	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	5	15	theme	adsorbent	909:917	arg1	's					918:919	the adsorbent's dosage, pH, initial concentration, and contact time	905:971	the adsorbent's dosage, pH, initial concentration, and contact time	905:971	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	5	15	theme	adsorbent	909:917	arg1	pH					929:930	pH	929:930	pH	929:930	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	5	15	theme	adsorbent	909:917	arg1	concentration					941:953	initial concentration	933:953	initial concentration	933:953	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	13	16	theme	Lagergren	1718:1726	arg1	kinetics					1748:1755	Lagergren pseudo-second-order kinetics	1718:1755	Lagergren pseudo-second-order kinetics	1718:1755	The rate of adsorption on beads follows Lagergren pseudo-second-order kinetics.					
36327083	3	17	theme	batch	708:712	arg1	approach					725:732	The batch adsorption approach	704:732	The batch adsorption approach	704:732	The batch adsorption approach was employed to remove the antibiotic from the water.					
36327083	1	18	theme	low-cost	431:438	arg1	beads					460:464	low-cost composite adsorbent beads	431:464	low-cost composite adsorbent beads	431:464	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	19	from	chitosan	153:160	arg1	combinations					231:242	different combinations	221:242	different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)	221:376	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	20	theme	present	138:144	arg1	study					146:150	the present study	134:150	the present study	134:150	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	4	21	theme	adsorption	853:862	arg1	mechanism					864:872	the adsorption mechanism	849:872	the adsorption mechanism	849:872	Moreover, isotherm and kinetics were conducted to illustrate the adsorption mechanism.					
36327083	8	22	theme	antibiotic	1193:1202	arg1	concentration					1204:1216	initial antibiotic concentration	1185:1216	initial antibiotic concentration	1185:1216	In addition, adsorption increased with initial antibiotic concentration and time rise.					
36327083	6	23	from	increase	1064:1071	arg1	dosage					1086:1091	adsorbent dosage	1076:1091	adsorbent dosage	1076:1091	Adsorption percentage increased slightly with the increase in adsorbent dosage.					
36327083	0	24	theme	gatifloxacin	74:85	arg1	compound					98:105	gatifloxacin antibiotic compound	74:105	gatifloxacin antibiotic compound from aqueous solutions	74:128	Chitosan beads coated with almond and walnut shells for the adsorption of gatifloxacin antibiotic compound from aqueous solutions.					
36327083	1	25	theme	chitosan	268:275	arg1	2:1:1					277:281	chitosan 2:1:1	268:281	chitosan 2:1:1 (AWC)	268:287	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	25	theme	chitosan	268:275	arg1	AWC					284:286	AWC	284:286	AWC	284:286	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	26	dep	antibiotics	485:495	arg1	gatifloxacin					497:508	gatifloxacin	497:508	antibiotics gatifloxacin (GAT)	485:514	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	26	dep	antibiotics	485:495	arg1	GAT					511:513	GAT	511:513	GAT	511:513	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	13	27	theme	pseudo-second-order	1728:1746	arg1	kinetics					1748:1755	Lagergren pseudo-second-order kinetics	1718:1755	Lagergren pseudo-second-order kinetics	1718:1755	The rate of adsorption on beads follows Lagergren pseudo-second-order kinetics.					
36327083	0	28	theme	compound	98:105	arg1	adsorption					60:69	the adsorption	56:69	the adsorption of gatifloxacin antibiotic compound from aqueous solutions	56:128	Chitosan beads coated with almond and walnut shells for the adsorption of gatifloxacin antibiotic compound from aqueous solutions.					
36327083	5	29	theme	contact	960:966	arg1	time					968:971	contact time	960:971	contact time	960:971	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	5	29	theme	contact	960:966	arg1	's					918:919	the adsorbent's dosage, pH, initial concentration, and contact time	905:971	the adsorbent's dosage, pH, initial concentration, and contact time	905:971	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	1	30	dep	combinations	231:242	arg1	walnut					261:266	walnut	261:266	different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)	221:376	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	30	dep	combinations	231:242	arg1	WAC					373:375	WAC	373:375	WAC	373:375	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	0	31	theme	Chitosan	0:7	arg1	beads					9:13	Chitosan beads	0:13	Chitosan beads	0:13	Chitosan beads coated with almond and walnut shells for the adsorption of gatifloxacin antibiotic compound from aqueous solutions.					
36327083	1	32	theme	powder	200:205	arg1	powder					379:384	almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder	183:384	almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder	183:384	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	33	theme	different	403:411	arg1	ratios					413:418	different ratios	403:418	different ratios	403:418	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	34	theme	adsorbent	450:458	arg1	beads					460:464	low-cost composite adsorbent beads	431:464	low-cost composite adsorbent beads	431:464	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	0	35	theme	almond	27:32	arg1	shells					45:50	almond and walnut shells	27:50	almond and walnut shells for the adsorption of gatifloxacin antibiotic compound from aqueous solutions	27:128	Chitosan beads coated with almond and walnut shells for the adsorption of gatifloxacin antibiotic compound from aqueous solutions.					
36327083	1	36	theme	different	221:229	arg1	combinations					231:242	different combinations	221:242	different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)	221:376	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	14	37	theme	combination	1793:1803	arg1	beads					1805:1809	prepared combination beads	1784:1809	prepared combination beads	1784:1809	The results indicate that prepared combination beads can be used to remove antibiotics from wastewater.					
36327083	2	38	dep	transform	615:623	arg1	infrared					625:632	infrared	625:632	transform infrared spectrum spectrophotometer	615:659	The beads were characterized by a scanning electron microscope, Fourier transform infrared spectrum spectrophotometer, and energy-dispersive X-ray spectroscopy.					
36327083	9	39	dep	AWC	1312:1314	arg1	beads					1324:1328	beads	1324:1328	beads	1324:1328	The adsorption isotherm data were successfully fitted to Langmuir isotherm for AWC and CAW beads, while WAC beads followed the Freundlich isotherm.					
36327083	0	40	theme	walnut	38:43	arg1	shells					45:50	almond and walnut shells	27:50	almond and walnut shells for the adsorption of gatifloxacin antibiotic compound from aqueous solutions	27:128	Chitosan beads coated with almond and walnut shells for the adsorption of gatifloxacin antibiotic compound from aqueous solutions.					
36327083	9	41	theme	adsorption	1237:1246	arg1	data					1257:1260	The adsorption isotherm data	1233:1260	The adsorption isotherm data	1233:1260	The adsorption isotherm data were successfully fitted to Langmuir isotherm for AWC and CAW beads, while WAC beads followed the Freundlich isotherm.					
36327083	2	42	theme	energy-dispersive	666:682	arg1	spectroscopy					690:701	energy-dispersive X-ray spectroscopy	666:701	energy-dispersive X-ray spectroscopy	666:701	The beads were characterized by a scanning electron microscope, Fourier transform infrared spectrum spectrophotometer, and energy-dispersive X-ray spectroscopy.					
36327083	1	43	theme	chitosan	357:364	arg1	2:1:1					366:370	chitosan 2:1:1	357:370	different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)	221:376	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	10	44	from	beads	1432:1436	arg1	beads					1462:1466	AWC and WAC beads	1450:1466	AWC and WAC beads	1450:1466	The highest adsorption was attained at pH 5 on CAW beads and pH 7 on AWC and WAC beads.					
36327083	13	45	from	rate	1682:1685	arg1	beads					1704:1708	beads	1704:1708	beads	1704:1708	The rate of adsorption on beads follows Lagergren pseudo-second-order kinetics.					
36327083	10	46	from	pH	1442:1443	arg1	beads					1462:1466	AWC and WAC beads	1450:1466	AWC and WAC beads	1450:1466	The highest adsorption was attained at pH 5 on CAW beads and pH 7 on AWC and WAC beads.					
36327083	12	47	theme	Langmuir	1614:1621	arg1	model					1623:1627	the Langmuir model and Freundlich adsorption	1610:1653	model	1623:1627	The adsorption isotherm data in AWC beads fit well with the Langmuir model and Freundlich adsorption for CAW and WAC beads.					
36327083	12	48	dep	CAW	1659:1661	arg1	beads					1671:1675	beads	1671:1675	beads	1671:1675	The adsorption isotherm data in AWC beads fit well with the Langmuir model and Freundlich adsorption for CAW and WAC beads.					
36327083	11	49	theme	contact	1481:1487	arg1	120 min					1522:1528	120 min	1522:1528	120 min	1522:1528	The optimal contact time for equilibrium studies was 120 min for all types of beads.					
36327083	11	49	theme	contact	1481:1487	arg1	time					1489:1492	The optimal contact time	1469:1492	The optimal contact time for equilibrium studies	1469:1516	The optimal contact time for equilibrium studies was 120 min for all types of beads.					
36327083	1	50	theme	shell	190:194	arg1	powder					379:384	almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder	183:384	almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder	183:384	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	12	51	theme	AWC	1586:1588	arg1	beads					1590:1594	AWC beads	1586:1594	AWC beads	1586:1594	The adsorption isotherm data in AWC beads fit well with the Langmuir model and Freundlich adsorption for CAW and WAC beads.					
36327083	7	52	theme	GAT	1113:1115	arg1	adsorption					1117:1126	GAT adsorption	1113:1126	GAT adsorption on beads	1113:1135	The optimum pH for GAT adsorption on beads was 5-7.					
36327083	1	53	theme	A	197:197	arg1	powder					379:384	almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder	183:384	almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder	183:384	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	7	54	from	adsorption	1117:1126	arg1	beads					1131:1135	beads	1131:1135	beads	1131:1135	The optimum pH for GAT adsorption on beads was 5-7.					
36327083	5	55	dep	's	918:919	arg1	dosage					921:926	dosage	921:926	dosage	921:926	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	5	55	dep	's	918:919	arg1	time					968:971	contact time	960:971	contact time	960:971	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	5	55	dep	's	918:919	arg1	's					918:919	the adsorbent's dosage, pH, initial concentration, and contact time	905:971	the adsorbent's dosage, pH, initial concentration, and contact time	905:971	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	5	55	dep	's	918:919	arg1	pH					929:930	pH	929:930	pH	929:930	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	5	55	dep	's	918:919	arg1	concentration					941:953	initial concentration	933:953	initial concentration	933:953	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	13	56	theme	adsorption	1690:1699	arg1	rate					1682:1685	The rate	1678:1685	The rate of adsorption on beads	1678:1708	The rate of adsorption on beads follows Lagergren pseudo-second-order kinetics.					
36327083	12	57	theme	adsorption	1558:1567	arg1	data					1578:1581	The adsorption isotherm data	1554:1581	The adsorption isotherm data in AWC beads	1554:1594	The adsorption isotherm data in AWC beads fit well with the Langmuir model and Freundlich adsorption for CAW and WAC beads.					
36327083	2	58	theme	electron	586:593	arg1	microscope					595:604	a scanning electron microscope	575:604	a scanning electron microscope	575:604	The beads were characterized by a scanning electron microscope, Fourier transform infrared spectrum spectrophotometer, and energy-dispersive X-ray spectroscopy.					
36327083	6	59	theme	Adsorption	1014:1023	arg1	percentage					1025:1034	Adsorption percentage	1014:1034	Adsorption percentage	1014:1034	Adsorption percentage increased slightly with the increase in adsorbent dosage.					
36327083	2	60	theme	spectrum	634:641	arg1	spectrophotometer					643:659	spectrum spectrophotometer	634:659	spectrum spectrophotometer	634:659	The beads were characterized by a scanning electron microscope, Fourier transform infrared spectrum spectrophotometer, and energy-dispersive X-ray spectroscopy.					
36327083	5	61	from	effect	895:900	arg1	adsorption					987:996	antibiotic adsorption	976:996	antibiotic adsorption	976:996	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	9	62	theme	WAC	1337:1339	arg1	beads					1341:1345	WAC beads	1337:1345	WAC beads	1337:1345	The adsorption isotherm data were successfully fitted to Langmuir isotherm for AWC and CAW beads, while WAC beads followed the Freundlich isotherm.					
36327083	1	63	theme	almond	247:252	arg1	shells					254:259	almond shells	247:259	almond shells	247:259	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	5	64	theme	's	918:919	arg1	effect					895:900	the effect	891:900	the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption	891:996	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	1	65	from	walnut	167:172	arg1	combinations					231:242	different combinations	221:242	different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)	221:376	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	9	66	theme	Freundlich	1360:1369	arg1	isotherm					1371:1378	the Freundlich isotherm	1356:1378	the Freundlich isotherm	1356:1378	The adsorption isotherm data were successfully fitted to Langmuir isotherm for AWC and CAW beads, while WAC beads followed the Freundlich isotherm.					
36327083	6	67	theme	adsorbent	1076:1084	arg1	dosage					1086:1091	adsorbent dosage	1076:1091	adsorbent dosage	1076:1091	Adsorption percentage increased slightly with the increase in adsorbent dosage.					
36327083	1	68	theme	composite	440:448	arg1	beads					460:464	low-cost composite adsorbent beads	431:464	low-cost composite adsorbent beads	431:464	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	7	69	theme	optimum	1098:1104	arg1	5-7					1141:1143	5-7	1141:1143	5-7	1141:1143	The optimum pH for GAT adsorption on beads was 5-7.					
36327083	7	69	theme	optimum	1098:1104	arg1	pH					1106:1107	The optimum pH	1094:1107	The optimum pH for GAT adsorption on beads	1094:1135	The optimum pH for GAT adsorption on beads was 5-7.					
36327083	1	70	theme	walnut	312:317	arg1	CAW					326:328	CAW	326:328	CAW	326:328	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	70	theme	walnut	312:317	arg1	2:1:1					319:323	walnut 2:1:1	312:323	chitosan:almond shell:walnut 2:1:1 (CAW)	290:329	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	3	71	theme	adsorption	714:723	arg1	approach					725:732	The batch adsorption approach	704:732	The batch adsorption approach	704:732	The batch adsorption approach was employed to remove the antibiotic from the water.					
36327083	1	72	from	powder	379:384	arg1	combinations					231:242	different combinations	221:242	different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)	221:376	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	5	73	theme	antibiotic	976:985	arg1	adsorption					987:996	antibiotic adsorption	976:996	antibiotic adsorption	976:996	Parameters like the effect of the adsorbent's dosage, pH, initial concentration, and contact time on antibiotic adsorption were evaluated.					
36327083	1	74	from	wastewater	531:540	arg1	removal					474:480	the removal	470:480	the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater	470:540	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	8	75	theme	initial	1185:1191	arg1	concentration					1204:1216	initial antibiotic concentration	1185:1216	initial antibiotic concentration	1185:1216	In addition, adsorption increased with initial antibiotic concentration and time rise.					
36327083	0	76	theme	antibiotic	87:96	arg1	compound					98:105	gatifloxacin antibiotic compound	74:105	gatifloxacin antibiotic compound from aqueous solutions	74:128	Chitosan beads coated with almond and walnut shells for the adsorption of gatifloxacin antibiotic compound from aqueous solutions.					
36327083	8	77	theme	time	1222:1225	arg1	rise					1227:1230	time rise	1222:1230	time rise	1222:1230	In addition, adsorption increased with initial antibiotic concentration and time rise.					
36327083	1	78	dep	walnut	261:266	arg1	chitosan					290:297	chitosan	290:297	chitosan:almond shell:walnut 2:1:1 (CAW)	290:329	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	78	dep	walnut	261:266	arg1	2:1:1					277:281	chitosan 2:1:1	268:281	chitosan 2:1:1 (AWC)	268:287	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	78	dep	walnut	261:266	arg1	shells					350:355	almond shells	343:355	different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)	221:376	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	78	dep	walnut	261:266	arg1	AWC					284:286	AWC	284:286	AWC	284:286	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	78	dep	walnut	261:266	arg1	walnut					336:341	walnut	336:341	walnut	336:341	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	78	dep	walnut	261:266	arg1	2:1:1					366:370	chitosan 2:1:1	357:370	different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)	221:376	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	79	theme	adsorbent	207:215	arg1	powder					379:384	almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder	183:384	almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder	183:384	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	12	80	from	data	1578:1581	arg1	beads					1590:1594	AWC beads	1586:1594	AWC beads	1586:1594	The adsorption isotherm data in AWC beads fit well with the Langmuir model and Freundlich adsorption for CAW and WAC beads.					
36327083	1	81	theme	almond	343:348	arg1	shells					350:355	almond shells	343:355	different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)	221:376	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	14	82	theme	prepared	1784:1791	arg1	beads					1805:1809	prepared combination beads	1784:1809	prepared combination beads	1784:1809	The results indicate that prepared combination beads can be used to remove antibiotics from wastewater.					
36327083	2	83	dep	Fourier	607:613	arg1	transform					615:623	transform	615:623	transform infrared spectrum spectrophotometer	615:659	The beads were characterized by a scanning electron microscope, Fourier transform infrared spectrum spectrophotometer, and energy-dispersive X-ray spectroscopy.					
36327083	10	84	theme	highest	1385:1391	arg1	adsorption					1393:1402	The highest adsorption	1381:1402	The highest adsorption	1381:1402	The highest adsorption was attained at pH 5 on CAW beads and pH 7 on AWC and WAC beads.					
36327083	11	85	theme	beads	1547:1551	arg1	types					1538:1542	all types	1534:1542	all types of beads	1534:1551	The optimal contact time for equilibrium studies was 120 min for all types of beads.					
36327083	9	86	theme	isotherm	1248:1255	arg1	data					1257:1260	The adsorption isotherm data	1233:1260	The adsorption isotherm data	1233:1260	The adsorption isotherm data were successfully fitted to Langmuir isotherm for AWC and CAW beads, while WAC beads followed the Freundlich isotherm.					
36327083	12	87	theme	Freundlich	1633:1642	arg1	adsorption					1644:1653	the Langmuir model and Freundlich adsorption	1610:1653	adsorption	1644:1653	The adsorption isotherm data in AWC beads fit well with the Langmuir model and Freundlich adsorption for CAW and WAC beads.					
36327083	1	88	dep	chitosan	290:297	arg1	CAW					326:328	CAW	326:328	CAW	326:328	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	88	dep	chitosan	290:297	arg1	shell					306:310	almond shell	299:310	chitosan:almond shell:walnut 2:1:1 (CAW)	290:329	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	1	88	dep	chitosan	290:297	arg1	2:1:1					319:323	walnut 2:1:1	312:323	chitosan:almond shell:walnut 2:1:1 (CAW)	290:329	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36327083	0	89	from	solutions	120:128	arg1	adsorption					60:69	the adsorption	56:69	the adsorption of gatifloxacin antibiotic compound from aqueous solutions	56:128	Chitosan beads coated with almond and walnut shells for the adsorption of gatifloxacin antibiotic compound from aqueous solutions.					
36327083	0	89	from	solutions	120:128	arg1	compound					98:105	gatifloxacin antibiotic compound	74:105	gatifloxacin antibiotic compound from aqueous solutions	74:128	Chitosan beads coated with almond and walnut shells for the adsorption of gatifloxacin antibiotic compound from aqueous solutions.					
36327083	11	90	theme	optimal	1473:1479	arg1	120 min					1522:1528	120 min	1522:1528	120 min	1522:1528	The optimal contact time for equilibrium studies was 120 min for all types of beads.					
36327083	11	90	theme	optimal	1473:1479	arg1	time					1489:1492	The optimal contact time	1469:1492	The optimal contact time for equilibrium studies	1469:1516	The optimal contact time for equilibrium studies was 120 min for all types of beads.					
36327083	1	91	theme	almond	183:188	arg1	powder					379:384	almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder	183:384	almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder	183:384	In the present study, chitosan (C), walnut (W), and almond shell (A) powder adsorbent (in different combinations as almond shells:walnut:chitosan 2:1:1 (AWC), chitosan:almond shell:walnut 2:1:1 (CAW), and walnut:almond shells:chitosan 2:1:1 (WAC)) powder were combined in different ratios to produce low-cost composite adsorbent beads for the removal of antibiotics gatifloxacin (GAT) from synthetic wastewater.					
36475598	6	0	theme	cross-linking	1012:1024	arg1	agents					1026:1031	cross-linking agents	1012:1031	cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3)	1012:1095	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	6	0	theme	cross-linking	1012:1024	arg1	periodate					1048:1056	sodium periodate	1041:1056	sodium periodate (NaIO4)	1041:1064	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	6	0	theme	cross-linking	1012:1024	arg1	trichloride					1077:1087	ferric trichloride	1070:1087	ferric trichloride (FeCl3)	1070:1095	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	6	1	theme	cross-linking	1180:1192	arg1	agents					1194:1199	the cross-linking agents	1176:1199	the cross-linking agents	1176:1199	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	3	2	theme	adhesives	511:519	arg1	series					481:486	a series	479:486	a series of injectable hydrogel adhesives to improve their cohesion and adhesion by in situ mixing DA with the polysaccharide without covalent modification	479:633	In this work, we aimed to prepare a series of injectable hydrogel adhesives to improve their cohesion and adhesion by in situ mixing DA with the polysaccharide without covalent modification.					
36475598	8	3	theme	non-covalent	1559:1570	arg1	bonds					1585:1589	non-covalent and covalent bonds	1559:1589	non-covalent and covalent bonds of DA	1559:1595	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	8	4	theme	covalent	1576:1583	arg1	bonds					1585:1589	non-covalent and covalent bonds	1559:1589	non-covalent and covalent bonds of DA	1559:1595	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	4	5	theme	DA	806:807	arg1	presence					781:788	the presence	777:788	the presence	777:788	The injectable and rapid curing adhesives were prepared by mixing oxidized dextran (ODE) and chitosan (CS) through a Schiff base reaction in the presence (or absence) of DA.					
36475598	4	5	theme	DA	806:807	arg1	absence					794:800	absence	794:800	absence	794:800	The injectable and rapid curing adhesives were prepared by mixing oxidized dextran (ODE) and chitosan (CS) through a Schiff base reaction in the presence (or absence) of DA.					
36475598	7	6	theme	fibrin	1338:1343	arg1	glue					1345:1348	fibrin glue	1338:1348	fibrin glue	1338:1348	The MC-FeCl3 hydrogel adhesive displayed good tissue adhesion with a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue.					
36475598	6	7	theme	ferric	1070:1075	arg1	FeCl3					1090:1094	FeCl3	1090:1094	FeCl3	1090:1094	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	6	7	theme	ferric	1070:1075	arg1	trichloride					1077:1087	ferric trichloride	1070:1087	ferric trichloride (FeCl3)	1070:1095	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	2	8	theme	adhesives	332:340	arg1	cohesion					301:308	Low cohesion	297:308	Low cohesion of the polysaccharide adhesives	297:340	Low cohesion of the polysaccharide adhesives and easy oxidation of DA may result in the low adhesion properties of the polysaccharide-DA adhesives.					
36475598	2	8	theme	adhesives	332:340	arg1	oxidation					351:359	easy oxidation	346:359	easy oxidation of DA	346:365	Low cohesion of the polysaccharide adhesives and easy oxidation of DA may result in the low adhesion properties of the polysaccharide-DA adhesives.					
36475598	4	9	theme	injectable	640:649	arg1	adhesives					668:676	The injectable and rapid curing adhesives	636:676	The injectable and rapid curing adhesives	636:676	The injectable and rapid curing adhesives were prepared by mixing oxidized dextran (ODE) and chitosan (CS) through a Schiff base reaction in the presence (or absence) of DA.					
36475598	1	10	from	derivatives	284:294	arg1	DA					251:252	DA	251:252	DA	251:252	Polysaccharide bio-adhesives used for non-invasive repair often show weak mechanical strength and tissue adhesion, even when covalently modified with dopamine (DA) from mussel proteins and its derivatives.					
36475598	1	10	from	derivatives	284:294	arg1	dopamine					241:248	dopamine	241:248	dopamine (DA) from mussel proteins and its derivatives	241:294	Polysaccharide bio-adhesives used for non-invasive repair often show weak mechanical strength and tissue adhesion, even when covalently modified with dopamine (DA) from mussel proteins and its derivatives.					
36475598	3	11	theme	in	563:564	arg1	DA					578:579	in situ mixing DA	563:579	in situ mixing DA	563:579	In this work, we aimed to prepare a series of injectable hydrogel adhesives to improve their cohesion and adhesion by in situ mixing DA with the polysaccharide without covalent modification.					
36475598	4	12	theme	rapid	655:659	arg1	adhesives					668:676	The injectable and rapid curing adhesives	636:676	The injectable and rapid curing adhesives	636:676	The injectable and rapid curing adhesives were prepared by mixing oxidized dextran (ODE) and chitosan (CS) through a Schiff base reaction in the presence (or absence) of DA.					
36475598	7	13	theme	shear	1275:1279	arg1	strength					1290:1297	a lap shear adhesion strength	1269:1297	a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue	1269:1348	The MC-FeCl3 hydrogel adhesive displayed good tissue adhesion with a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue.					
36475598	6	14	theme	sol-gel	1108:1114	arg1	transitions					1116:1126	their sol-gel transitions	1102:1126	their sol-gel transitions	1102:1126	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	1	15	theme	mechanical	165:174	arg1	strength					176:183	weak mechanical strength	160:183	weak mechanical strength	160:183	Polysaccharide bio-adhesives used for non-invasive repair often show weak mechanical strength and tissue adhesion, even when covalently modified with dopamine (DA) from mussel proteins and its derivatives.					
36475598	1	16	theme	weak	160:163	arg1	strength					176:183	weak mechanical strength	160:183	weak mechanical strength	160:183	Polysaccharide bio-adhesives used for non-invasive repair often show weak mechanical strength and tissue adhesion, even when covalently modified with dopamine (DA) from mussel proteins and its derivatives.					
36475598	8	17	theme	Raman	1364:1368	arg1	spectra					1370:1376	Raman spectra	1364:1376	Raman spectra	1364:1376	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	3	18	theme	covalent	613:620	arg1	modification					622:633	covalent modification	613:633	covalent modification	613:633	In this work, we aimed to prepare a series of injectable hydrogel adhesives to improve their cohesion and adhesion by in situ mixing DA with the polysaccharide without covalent modification.					
36475598	3	19	dep	in	563:564	arg1	situ					566:569	situ	566:569	situ	566:569	In this work, we aimed to prepare a series of injectable hydrogel adhesives to improve their cohesion and adhesion by in situ mixing DA with the polysaccharide without covalent modification.					
36475598	8	20	theme	profile	1387:1393	arg1	analyses					1395:1402	texture profile analyses	1379:1402	texture profile analyses	1379:1402	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	4	21	theme	base	760:763	arg1	reaction					765:772	a Schiff base reaction	751:772	a Schiff base reaction in the presence (or absence) of DA	751:807	The injectable and rapid curing adhesives were prepared by mixing oxidized dextran (ODE) and chitosan (CS) through a Schiff base reaction in the presence (or absence) of DA.					
36475598	7	22	theme	tissue	1248:1253	arg1	adhesion					1255:1262	good tissue adhesion	1243:1262	good tissue adhesion	1243:1262	The MC-FeCl3 hydrogel adhesive displayed good tissue adhesion with a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue.					
36475598	8	23	theme	atomic	1409:1414	arg1	microscopy					1422:1431	atomic force microscopy	1409:1431	atomic force microscopy images	1409:1438	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	2	24	theme	adhesion	389:396	arg1	properties					398:407	the low adhesion properties	381:407	the low adhesion properties of the polysaccharide-DA adhesives	381:442	Low cohesion of the polysaccharide adhesives and easy oxidation of DA may result in the low adhesion properties of the polysaccharide-DA adhesives.					
36475598	0	25	theme	Injectable	0:9	arg1	Hydrogels					53:61	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels	0:61	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels	0:61	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels with Enhanced Adhesiveness.					
36475598	8	26	theme	microscopy	1422:1431	arg1	images					1433:1438	atomic force microscopy images	1409:1438	atomic force microscopy images	1409:1438	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	0	27	theme	In	35:36	arg1	Hydrogels					53:61	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels	0:61	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels	0:61	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels with Enhanced Adhesiveness.					
36475598	2	28	theme	Low	297:299	arg1	cohesion					301:308	Low cohesion	297:308	Low cohesion of the polysaccharide adhesives	297:340	Low cohesion of the polysaccharide adhesives and easy oxidation of DA may result in the low adhesion properties of the polysaccharide-DA adhesives.					
36475598	10	29	theme	good	1745:1748	arg1	adhesion					1750:1757	good adhesion	1745:1757	good adhesion	1745:1757	These results demonstrate that the injectable and sticky hydrogels with good adhesion are promising materials for tissue repair.					
36475598	2	30	theme	polysaccharide-DA	416:432	arg1	adhesives					434:442	the polysaccharide-DA adhesives	412:442	the polysaccharide-DA adhesives	412:442	Low cohesion of the polysaccharide adhesives and easy oxidation of DA may result in the low adhesion properties of the polysaccharide-DA adhesives.					
36475598	0	31	theme	Composite	43:51	arg1	Hydrogels					53:61	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels	0:61	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels	0:61	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels with Enhanced Adhesiveness.					
36475598	5	32	theme	DA	942:943	arg1	DA					942:943	DA	942:943	DA	942:943	The gelation time of the adhesive was customized to be less than 20 s by controlling the amount of ODE, regardless of the amount of DA.					
36475598	5	32	theme	DA	942:943	arg1	amount					932:937	the amount	928:937	the amount of DA	928:943	The gelation time of the adhesive was customized to be less than 20 s by controlling the amount of ODE, regardless of the amount of DA.					
36475598	8	33	theme	enhanced	1454:1461	arg1	adhesion					1463:1470	the enhanced adhesion	1450:1470	the enhanced adhesion induced by a higher cohesion of MC-FeCl3	1450:1511	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	2	34	theme	DA	364:365	arg1	cohesion					301:308	Low cohesion	297:308	Low cohesion of the polysaccharide adhesives	297:340	Low cohesion of the polysaccharide adhesives and easy oxidation of DA may result in the low adhesion properties of the polysaccharide-DA adhesives.					
36475598	2	34	theme	DA	364:365	arg1	oxidation					351:359	easy oxidation	346:359	easy oxidation of DA	346:365	Low cohesion of the polysaccharide adhesives and easy oxidation of DA may result in the low adhesion properties of the polysaccharide-DA adhesives.					
36475598	10	35	with	hydrogels	1730:1738	arg1	adhesion					1750:1757	good adhesion	1745:1757	good adhesion	1745:1757	These results demonstrate that the injectable and sticky hydrogels with good adhesion are promising materials for tissue repair.					
36475598	0	36	dep	In	35:36	arg1	Situ					38:41	Situ	38:41	Situ	38:41	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels with Enhanced Adhesiveness.					
36475598	6	37	theme	agents	1194:1199	arg1	amounts					1165:1171	the amounts	1161:1171	the amounts of the cross-linking agents	1161:1199	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	6	37	theme	agents	1194:1199	arg1	agents					1194:1199	the cross-linking agents	1176:1199	the cross-linking agents	1176:1199	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	3	38	theme	hydrogel	502:509	arg1	adhesives					511:519	injectable hydrogel adhesives	491:519	injectable hydrogel adhesives	491:519	In this work, we aimed to prepare a series of injectable hydrogel adhesives to improve their cohesion and adhesion by in situ mixing DA with the polysaccharide without covalent modification.					
36475598	8	39	theme	MC-FeCl3	1504:1511	arg1	cohesion					1492:1499	a higher cohesion	1483:1499	a higher cohesion of MC-FeCl3	1483:1511	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	10	40	theme	tissue	1787:1792	arg1	repair					1794:1799	tissue repair	1787:1799	tissue repair	1787:1799	These results demonstrate that the injectable and sticky hydrogels with good adhesion are promising materials for tissue repair.					
36475598	8	41	from	images	1433:1438	arg1	Results					1351:1357	Results	1351:1357	Results from Raman spectra, texture profile analyses, and atomic force microscopy images	1351:1438	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	7	42	theme	glue	1345:1348	arg1	times					1324:1328	43 times	1321:1328	43 times that of fibrin glue	1321:1348	The MC-FeCl3 hydrogel adhesive displayed good tissue adhesion with a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue.					
36475598	6	43	link	Multi-cross-linked	946:963	arg1	hydrogels					970:978	Multi-cross-linked (MC) hydrogels	946:978	Multi-cross-linked (MC) hydrogels	946:978	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	6	43	link	Multi-cross-linked	946:963	arg1	MC					966:967	MC	966:967	MC	966:967	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	5	44	theme	gelation	814:821	arg1	customized					848:857	customized	848:857	customized	848:857	The gelation time of the adhesive was customized to be less than 20 s by controlling the amount of ODE, regardless of the amount of DA.					
36475598	5	44	theme	gelation	814:821	arg1	time					823:826	The gelation time	810:826	The gelation time of the adhesive	810:842	The gelation time of the adhesive was customized to be less than 20 s by controlling the amount of ODE, regardless of the amount of DA.					
36475598	5	44	theme	gelation	814:821	arg1	s					878:878	less than 20 s	865:878	less than 20 s	865:878	The gelation time of the adhesive was customized to be less than 20 s by controlling the amount of ODE, regardless of the amount of DA.					
36475598	4	45	theme	curing	661:666	arg1	adhesives					668:676	The injectable and rapid curing adhesives	636:676	The injectable and rapid curing adhesives	636:676	The injectable and rapid curing adhesives were prepared by mixing oxidized dextran (ODE) and chitosan (CS) through a Schiff base reaction in the presence (or absence) of DA.					
36475598	2	46	theme	polysaccharide	317:330	arg1	adhesives					332:340	the polysaccharide adhesives	313:340	the polysaccharide adhesives	313:340	Low cohesion of the polysaccharide adhesives and easy oxidation of DA may result in the low adhesion properties of the polysaccharide-DA adhesives.					
36475598	8	47	theme	DA	1594:1595	arg1	bonds					1585:1589	non-covalent and covalent bonds	1559:1589	non-covalent and covalent bonds of DA	1559:1595	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	8	47	theme	DA	1594:1595	arg1	coordination					1527:1538	the coordination	1523:1538	the coordination of Fe3+ and DA	1523:1553	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	3	48	theme	mixing	571:576	arg1	DA					578:579	in situ mixing DA	563:579	in situ mixing DA	563:579	In this work, we aimed to prepare a series of injectable hydrogel adhesives to improve their cohesion and adhesion by in situ mixing DA with the polysaccharide without covalent modification.					
36475598	7	49	theme	kPa	1306:1308	arg1	strength					1290:1297	a lap shear adhesion strength	1269:1297	a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue	1269:1348	The MC-FeCl3 hydrogel adhesive displayed good tissue adhesion with a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue.					
36475598	1	50	from	proteins	267:274	arg1	DA					251:252	DA	251:252	DA	251:252	Polysaccharide bio-adhesives used for non-invasive repair often show weak mechanical strength and tissue adhesion, even when covalently modified with dopamine (DA) from mussel proteins and its derivatives.					
36475598	1	50	from	proteins	267:274	arg1	dopamine					241:248	dopamine	241:248	dopamine (DA) from mussel proteins and its derivatives	241:294	Polysaccharide bio-adhesives used for non-invasive repair often show weak mechanical strength and tissue adhesion, even when covalently modified with dopamine (DA) from mussel proteins and its derivatives.					
36475598	7	51	theme	adhesion	1281:1288	arg1	strength					1290:1297	a lap shear adhesion strength	1269:1297	a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue	1269:1348	The MC-FeCl3 hydrogel adhesive displayed good tissue adhesion with a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue.					
36475598	10	52	theme	sticky	1723:1728	arg1	hydrogels					1730:1738	the injectable and sticky hydrogels	1704:1738	the injectable and sticky hydrogels with good adhesion	1704:1757	These results demonstrate that the injectable and sticky hydrogels with good adhesion are promising materials for tissue repair.					
36475598	10	52	theme	sticky	1723:1728	arg1	materials					1773:1781	promising materials	1763:1781	promising materials for tissue repair	1763:1799	These results demonstrate that the injectable and sticky hydrogels with good adhesion are promising materials for tissue repair.					
36475598	7	53	dep	times	1324:1328	arg1	that					1330:1333	that	1330:1333	that	1330:1333	The MC-FeCl3 hydrogel adhesive displayed good tissue adhesion with a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue.					
36475598	6	54	theme	Multi-cross-linked	946:963	arg1	hydrogels					970:978	Multi-cross-linked (MC) hydrogels	946:978	Multi-cross-linked (MC) hydrogels	946:978	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	6	54	theme	Multi-cross-linked	946:963	arg1	MC					966:967	MC	966:967	MC	966:967	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	7	55	theme	lap	1271:1273	arg1	strength					1290:1297	a lap shear adhesion strength	1269:1297	a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue	1269:1348	The MC-FeCl3 hydrogel adhesive displayed good tissue adhesion with a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue.					
36475598	10	56	theme	injectable	1708:1717	arg1	hydrogels					1730:1738	the injectable and sticky hydrogels	1704:1738	the injectable and sticky hydrogels with good adhesion	1704:1757	These results demonstrate that the injectable and sticky hydrogels with good adhesion are promising materials for tissue repair.					
36475598	10	56	theme	injectable	1708:1717	arg1	materials					1773:1781	promising materials	1763:1781	promising materials for tissue repair	1763:1799	These results demonstrate that the injectable and sticky hydrogels with good adhesion are promising materials for tissue repair.					
36475598	5	57	theme	adhesive	835:842	arg1	customized					848:857	customized	848:857	customized	848:857	The gelation time of the adhesive was customized to be less than 20 s by controlling the amount of ODE, regardless of the amount of DA.					
36475598	5	57	theme	adhesive	835:842	arg1	time					823:826	The gelation time	810:826	The gelation time of the adhesive	810:842	The gelation time of the adhesive was customized to be less than 20 s by controlling the amount of ODE, regardless of the amount of DA.					
36475598	5	57	theme	adhesive	835:842	arg1	s					878:878	less than 20 s	865:878	less than 20 s	865:878	The gelation time of the adhesive was customized to be less than 20 s by controlling the amount of ODE, regardless of the amount of DA.					
36475598	8	58	theme	texture	1379:1385	arg1	analyses					1395:1402	texture profile analyses	1379:1402	texture profile analyses	1379:1402	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	4	59	from	reaction	765:772	arg1	presence					781:788	the presence	777:788	the presence	777:788	The injectable and rapid curing adhesives were prepared by mixing oxidized dextran (ODE) and chitosan (CS) through a Schiff base reaction in the presence (or absence) of DA.					
36475598	4	59	from	reaction	765:772	arg1	absence					794:800	absence	794:800	absence	794:800	The injectable and rapid curing adhesives were prepared by mixing oxidized dextran (ODE) and chitosan (CS) through a Schiff base reaction in the presence (or absence) of DA.					
36475598	1	60	theme	tissue	189:194	arg1	adhesion					196:203	tissue adhesion	189:203	tissue adhesion	189:203	Polysaccharide bio-adhesives used for non-invasive repair often show weak mechanical strength and tissue adhesion, even when covalently modified with dopamine (DA) from mussel proteins and its derivatives.					
36475598	9	61	theme	good	1629:1632	arg1	biodegradability					1634:1649	good biodegradability	1629:1649	good biodegradability	1629:1649	Moreover, the adhesives showed good biodegradability and biocompatibility.					
36475598	4	62	theme	oxidized	702:709	arg1	ODE					720:722	ODE	720:722	ODE	720:722	The injectable and rapid curing adhesives were prepared by mixing oxidized dextran (ODE) and chitosan (CS) through a Schiff base reaction in the presence (or absence) of DA.					
36475598	4	62	theme	oxidized	702:709	arg1	dextran					711:717	oxidized dextran	702:717	oxidized dextran (ODE)	702:723	The injectable and rapid curing adhesives were prepared by mixing oxidized dextran (ODE) and chitosan (CS) through a Schiff base reaction in the presence (or absence) of DA.					
36475598	8	63	from	spectra	1370:1376	arg1	Results					1351:1357	Results	1351:1357	Results from Raman spectra, texture profile analyses, and atomic force microscopy images	1351:1438	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	1	64	theme	Polysaccharide	91:104	arg1	bio-adhesives					106:118	Polysaccharide bio-adhesives	91:118	Polysaccharide bio-adhesives used for non-invasive repair	91:147	Polysaccharide bio-adhesives used for non-invasive repair often show weak mechanical strength and tissue adhesion, even when covalently modified with dopamine (DA) from mussel proteins and its derivatives.					
36475598	7	65	theme	good	1243:1246	arg1	adhesion					1255:1262	good tissue adhesion	1243:1262	good tissue adhesion	1243:1262	The MC-FeCl3 hydrogel adhesive displayed good tissue adhesion with a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue.					
36475598	8	66	theme	force	1416:1420	arg1	microscopy					1422:1431	atomic force microscopy	1409:1431	atomic force microscopy images	1409:1438	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	4	67	theme	Schiff	753:758	arg1	reaction					765:772	a Schiff base reaction	751:772	a Schiff base reaction in the presence (or absence) of DA	751:807	The injectable and rapid curing adhesives were prepared by mixing oxidized dextran (ODE) and chitosan (CS) through a Schiff base reaction in the presence (or absence) of DA.					
36475598	0	68	theme	Dopamine-Polysaccharide	11:33	arg1	Hydrogels					53:61	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels	0:61	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels	0:61	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels with Enhanced Adhesiveness.					
36475598	7	69	theme	adhesive	1224:1231	arg1	hydrogel					1215:1222	The MC-FeCl3 hydrogel adhesive	1202:1231	The MC-FeCl3 hydrogel adhesive	1202:1231	The MC-FeCl3 hydrogel adhesive displayed good tissue adhesion with a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue.					
36475598	2	70	theme	low	385:387	arg1	properties					398:407	the low adhesion properties	381:407	the low adhesion properties of the polysaccharide-DA adhesives	381:442	Low cohesion of the polysaccharide adhesives and easy oxidation of DA may result in the low adhesion properties of the polysaccharide-DA adhesives.					
36475598	1	71	theme	non-invasive	129:140	arg1	repair					142:147	non-invasive repair	129:147	non-invasive repair	129:147	Polysaccharide bio-adhesives used for non-invasive repair often show weak mechanical strength and tissue adhesion, even when covalently modified with dopamine (DA) from mussel proteins and its derivatives.					
36475598	7	72	theme	MC-FeCl3	1206:1213	arg1	hydrogel					1215:1222	The MC-FeCl3 hydrogel adhesive	1202:1231	The MC-FeCl3 hydrogel adhesive	1202:1231	The MC-FeCl3 hydrogel adhesive displayed good tissue adhesion with a lap shear adhesion strength of 345 kPa, which was 43 times that of fibrin glue.					
36475598	5	73	theme	ODE	909:911	arg1	ODE					909:911	ODE	909:911	ODE	909:911	The gelation time of the adhesive was customized to be less than 20 s by controlling the amount of ODE, regardless of the amount of DA.					
36475598	5	73	theme	ODE	909:911	arg1	amount					899:904	the amount	895:904	the amount of ODE	895:911	The gelation time of the adhesive was customized to be less than 20 s by controlling the amount of ODE, regardless of the amount of DA.					
36475598	2	74	theme	adhesives	434:442	arg1	properties					398:407	the low adhesion properties	381:407	the low adhesion properties of the polysaccharide-DA adhesives	381:442	Low cohesion of the polysaccharide adhesives and easy oxidation of DA may result in the low adhesion properties of the polysaccharide-DA adhesives.					
36475598	0	75	theme	Enhanced	68:75	arg1	Adhesiveness					77:88	Enhanced Adhesiveness	68:88	Enhanced Adhesiveness	68:88	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels with Enhanced Adhesiveness.					
36475598	0	76	dep	Hydrogels	53:61	arg1	Adhesiveness					77:88	Enhanced Adhesiveness	68:88	Enhanced Adhesiveness	68:88	Injectable Dopamine-Polysaccharide In Situ Composite Hydrogels with Enhanced Adhesiveness.					
36475598	3	77	theme	injectable	491:500	arg1	adhesives					511:519	injectable hydrogel adhesives	491:519	injectable hydrogel adhesives	491:519	In this work, we aimed to prepare a series of injectable hydrogel adhesives to improve their cohesion and adhesion by in situ mixing DA with the polysaccharide without covalent modification.					
36475598	6	78	theme	sodium	1041:1046	arg1	NaIO4					1059:1063	NaIO4	1059:1063	NaIO4	1059:1063	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	6	78	theme	sodium	1041:1046	arg1	periodate					1048:1056	sodium periodate	1041:1056	sodium periodate (NaIO4)	1041:1064	Multi-cross-linked (MC) hydrogels were further prepared by adding cross-linking agents such as sodium periodate (NaIO4) and ferric trichloride (FeCl3), and their sol-gel transitions were easily adjusted by changing the amounts of the cross-linking agents.					
36475598	8	79	theme	higher	1485:1490	arg1	cohesion					1492:1499	a higher cohesion	1483:1499	a higher cohesion of MC-FeCl3	1483:1511	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	10	80	theme	promising	1763:1771	arg1	hydrogels					1730:1738	the injectable and sticky hydrogels	1704:1738	the injectable and sticky hydrogels with good adhesion	1704:1757	These results demonstrate that the injectable and sticky hydrogels with good adhesion are promising materials for tissue repair.					
36475598	10	80	theme	promising	1763:1771	arg1	materials					1773:1781	promising materials	1763:1781	promising materials for tissue repair	1763:1799	These results demonstrate that the injectable and sticky hydrogels with good adhesion are promising materials for tissue repair.					
36475598	2	81	theme	easy	346:349	arg1	oxidation					351:359	easy oxidation	346:359	easy oxidation of DA	346:365	Low cohesion of the polysaccharide adhesives and easy oxidation of DA may result in the low adhesion properties of the polysaccharide-DA adhesives.					
36475598	8	82	theme	Fe3+	1543:1546	arg1	bonds					1585:1589	non-covalent and covalent bonds	1559:1589	non-covalent and covalent bonds of DA	1559:1595	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	8	82	theme	Fe3+	1543:1546	arg1	coordination					1527:1538	the coordination	1523:1538	the coordination of Fe3+ and DA	1523:1553	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	8	83	from	analyses	1395:1402	arg1	Results					1351:1357	Results	1351:1357	Results from Raman spectra, texture profile analyses, and atomic force microscopy images	1351:1438	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	8	84	theme	DA	1552:1553	arg1	bonds					1585:1589	non-covalent and covalent bonds	1559:1589	non-covalent and covalent bonds of DA	1559:1595	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	8	84	theme	DA	1552:1553	arg1	coordination					1527:1538	the coordination	1523:1538	the coordination of Fe3+ and DA	1523:1553	Results from Raman spectra, texture profile analyses, and atomic force microscopy images confirmed the enhanced adhesion induced by a higher cohesion of MC-FeCl3, owing to the coordination of Fe3+ and DA and non-covalent and covalent bonds of DA.					
36475598	1	85	theme	mussel	260:265	arg1	proteins					267:274	mussel proteins	260:274	mussel proteins	260:274	Polysaccharide bio-adhesives used for non-invasive repair often show weak mechanical strength and tissue adhesion, even when covalently modified with dopamine (DA) from mussel proteins and its derivatives.					
35309318	4	0	from	level	654:658	arg1	lamprey					676:682	the reissner lamprey	663:682	the reissner lamprey (Lethenteron reissneri)	663:706	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	0	from	level	654:658	arg1	japonica					782:789	Lampetra japonica	773:789	Lampetra japonica	773:789	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	0	from	level	654:658	arg1	lamprey					717:723	the sea lamprey	709:723	the sea lamprey (Petromyzon marinus)	709:744	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	0	from	level	654:658	arg1	reissneri					697:705	reissneri	697:705	reissneri	697:705	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	0	from	level	654:658	arg1	lamprey					764:770	the Japanese lamprey	751:770	the Japanese lamprey (Lampetra japonica)	751:790	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	0	from	level	654:658	arg1	marinus					737:743	Petromyzon marinus	726:743	Petromyzon marinus	726:743	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	1	1	theme	behaviors	247:255	arg1	range					229:233	a wide range	222:233	a wide range of cellular behaviors	222:255	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	7	2	theme	recombinant	1422:1432	arg1	protein					1434:1440	the lamprey TGF-β2 recombinant protein	1403:1440	the lamprey TGF-β2 recombinant protein	1403:1440	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	4	3	theme	extant	807:812	arg1	representatives					814:828	the extant representatives	803:828	the extant representatives of jawless vertebrates with a history of more than 350 million years	803:897	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	3	theme	extant	807:812	arg1	level					654:658	the genome-wide level	638:658	the genome-wide level	638:658	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	7	4	theme	BrdU	1379:1382	arg1	assay					1384:1388	BrdU assay	1379:1388	BrdU assay	1379:1388	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	6	5	theme	innate	1199:1204	arg1	response					1213:1220	the innate immune response	1195:1220	the innate immune response of the lamprey	1195:1235	The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli.					
35309318	7	6	theme	lamprey	1407:1413	arg1	protein					1434:1440	the lamprey TGF-β2 recombinant protein	1403:1440	the lamprey TGF-β2 recombinant protein	1403:1440	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	6	7	theme	expression	1108:1117	arg1	patterns					1119:1126	The transcriptional expression patterns	1088:1126	The transcriptional expression patterns	1088:1126	The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli.					
35309318	9	8	theme	composite	1878:1886	arg1	results					1888:1894	Our composite results	1874:1894	Our composite results	1874:1894	Our composite results offer valuable clues to the origin and evolution of the TGF-β subfamily and imply that TGF-βs are among the most ancestral immune regulators in vertebrates.					
35309318	6	9	theme	regulatory	1176:1185	arg1	role					1187:1190	a central regulatory role	1166:1190	a central regulatory role	1166:1190	The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli.					
35309318	1	10	theme	multifunctional	174:188	arg1	factor-βs					151:159	The transforming growth factor-βs	127:159	The transforming growth factor-βs (TGF-βs)	127:168	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	1	10	theme	multifunctional	174:188	arg1	cytokines					190:198	multifunctional cytokines	174:198	multifunctional cytokines capable of regulating a wide range of cellular behaviors	174:255	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	0	11	theme	Lamprey	111:117	arg1	Characterization					91:106	the Functional Characterization	76:106	the Functional Characterization of Lamprey TGF-β2	76:124	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2.					
35309318	0	11	theme	Lamprey	111:117	arg1	Evolution					10:18	Molecular Evolution	0:18	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family	0:70	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2.					
35309318	3	12	theme	subfamily	526:534	arg1	processes					503:511	the evolutionary processes	486:511	the evolutionary processes of the TGF-β subfamily in vertebrates	486:549	However, the evolutionary processes of the TGF-β subfamily in vertebrates still lack sufficient elucidation.					
35309318	4	13	with	vertebrates	841:851	arg1	history					860:866	a history	858:866	a history of more than 350 million years	858:897	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	2	14	from	members	447:453	arg1	vertebrates					464:474	jawed vertebrates	458:474	jawed vertebrates	458:474	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	6	15	theme	central	1168:1174	arg1	role					1187:1190	a central regulatory role	1166:1190	a central regulatory role	1166:1190	The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli.					
35309318	5	16	theme	TGF-β2	1023:1028	arg1	ancestors					1010:1018	ancestors	1010:1018	ancestors of TGF-β2 and 3 in vertebrates, respectively	1010:1063	The molecular evolutionary analyses reveal that the lamprey TGF-β subfamily contains two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively, but TGF-β1 is absent.					
35309318	8	17	theme	caspase-3/7	1701:1711	arg1	analysis					1722:1729	caspase-3/7 activity analysis	1701:1729	caspase-3/7 activity analysis	1701:1729	Furthermore, caspase-3/7 activity analysis indicates that the lamprey TGF-β2 protects SMB cells from apoptosis after serum deprivation, in contrast to promoting apoptosis of leukocytes.					
35309318	4	18	theme	vertebrates	841:851	arg1	representatives					814:828	the extant representatives	803:828	the extant representatives of jawless vertebrates with a history of more than 350 million years	803:897	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	18	theme	vertebrates	841:851	arg1	level					654:658	the genome-wide level	638:658	the genome-wide level	638:658	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	2	19	located	present	365:371	arg1	deuterostomes					376:388	deuterostomes	376:388	deuterostomes	376:388	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	2	19	located	present	365:371	arg2	subfamily					340:348	The TGF-β subfamily	330:348	The TGF-β subfamily	330:348	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	2	19	located	present	365:371	arg2	present					365:371	present	365:371	present	365:371	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	7	20	from	proliferation	1623:1635	arg1	lampreys					1678:1685	lampreys	1678:1685	lampreys	1678:1685	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	6	21	theme	TGF-β3	1316:1321	arg1	stimuli					1349:1355	TGF-β3 during lipopolysaccharide stimuli	1316:1355	TGF-β3 during lipopolysaccharide stimuli	1316:1355	The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli.					
35309318	8	22	theme	lamprey	1750:1756	arg1	TGF-β2					1758:1763	the lamprey TGF-β2	1746:1763	the lamprey TGF-β2	1746:1763	Furthermore, caspase-3/7 activity analysis indicates that the lamprey TGF-β2 protects SMB cells from apoptosis after serum deprivation, in contrast to promoting apoptosis of leukocytes.					
35309318	1	23	theme	growth	144:149	arg1	TGF-βs					162:167	TGF-βs	162:167	TGF-βs	162:167	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	1	23	theme	growth	144:149	arg1	factor-βs					151:159	The transforming growth factor-βs	127:159	The transforming growth factor-βs (TGF-βs)	127:168	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	1	23	theme	growth	144:149	arg1	cytokines					190:198	multifunctional cytokines	174:198	multifunctional cytokines capable of regulating a wide range of cellular behaviors	174:255	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	7	24	theme	activated	1654:1662	arg1	leukocytes					1664:1673	quiescent and activated leukocytes	1640:1673	quiescent and activated leukocytes in lampreys	1640:1685	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	7	25	theme	SMB	1532:1534	arg1	cells					1525:1529	the supraneural myeloid body cells	1496:1529	the supraneural myeloid body cells (SMB cells)	1496:1541	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	7	25	theme	SMB	1532:1534	arg1	cells					1536:1540	SMB cells	1532:1540	SMB cells	1532:1540	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	8	26	dep	promoting	1839:1847	arg1	contrast					1827:1834	contrast	1827:1834	contrast	1827:1834	Furthermore, caspase-3/7 activity analysis indicates that the lamprey TGF-β2 protects SMB cells from apoptosis after serum deprivation, in contrast to promoting apoptosis of leukocytes.					
35309318	9	27	theme	TGF-β	1952:1956	arg1	subfamily					1958:1966	the TGF-β subfamily	1948:1966	the TGF-β subfamily	1948:1966	Our composite results offer valuable clues to the origin and evolution of the TGF-β subfamily and imply that TGF-βs are among the most ancestral immune regulators in vertebrates.					
35309318	3	28	from	processes	503:511	arg1	vertebrates					539:549	vertebrates	539:549	vertebrates	539:549	However, the evolutionary processes of the TGF-β subfamily in vertebrates still lack sufficient elucidation.					
35309318	0	29	theme	Family	65:70	arg1	Characterization					91:106	the Functional Characterization	76:106	the Functional Characterization of Lamprey TGF-β2	76:124	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2.					
35309318	0	29	theme	Family	65:70	arg1	Evolution					10:18	Molecular Evolution	0:18	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family	0:70	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2.					
35309318	1	30	theme	system	322:327	arg1	homeostasis					296:306	the homeostasis	292:306	the homeostasis of the immune system	292:327	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	7	31	theme	quiescent	1640:1648	arg1	leukocytes					1664:1673	quiescent and activated leukocytes	1640:1673	quiescent and activated leukocytes in lampreys	1640:1685	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	7	32	theme	cells	1525:1529	arg1	proliferation					1479:1491	the proliferation	1475:1491	the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state	1475:1582	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	9	33	from	regulators	2026:2035	arg1	vertebrates					2040:2050	vertebrates	2040:2050	vertebrates	2040:2050	Our composite results offer valuable clues to the origin and evolution of the TGF-β subfamily and imply that TGF-βs are among the most ancestral immune regulators in vertebrates.					
35309318	5	34	theme	3	1034:1034	arg1	ancestors					1010:1018	ancestors	1010:1018	ancestors of TGF-β2 and 3 in vertebrates, respectively	1010:1063	The molecular evolutionary analyses reveal that the lamprey TGF-β subfamily contains two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively, but TGF-β1 is absent.					
35309318	7	35	theme	myeloid	1512:1518	arg1	cells					1525:1529	the supraneural myeloid body cells	1496:1529	the supraneural myeloid body cells (SMB cells)	1496:1541	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	7	35	theme	myeloid	1512:1518	arg1	cells					1536:1540	SMB cells	1532:1540	SMB cells	1532:1540	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	4	36	theme	million	885:891	arg1	years					893:897	more than 350 million years	871:897	more than 350 million years	871:897	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	37	theme	Petromyzon	726:735	arg1	lamprey					717:723	the sea lamprey	709:723	the sea lamprey (Petromyzon marinus)	709:744	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	37	theme	Petromyzon	726:735	arg1	marinus					737:743	Petromyzon marinus	726:743	Petromyzon marinus	726:743	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	5	38	theme	molecular	904:912	arg1	analyses					927:934	The molecular evolutionary analyses	900:934	The molecular evolutionary analyses	900:934	The molecular evolutionary analyses reveal that the lamprey TGF-β subfamily contains two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively, but TGF-β1 is absent.					
35309318	0	39	theme	Molecular	0:8	arg1	Evolution					10:18	Molecular Evolution	0:18	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family	0:70	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2.					
35309318	8	40	theme	serum	1805:1809	arg1	deprivation					1811:1821	serum deprivation	1805:1821	serum deprivation	1805:1821	Furthermore, caspase-3/7 activity analysis indicates that the lamprey TGF-β2 protects SMB cells from apoptosis after serum deprivation, in contrast to promoting apoptosis of leukocytes.					
35309318	7	41	theme	inhibitory	1600:1609	arg1	role					1611:1614	an inhibitory role	1597:1614	an inhibitory role	1597:1614	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	2	42	theme	multiple	438:445	arg1	members					447:453	multiple members	438:453	multiple members in jawed vertebrates	438:474	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	9	43	theme	ancestral	2009:2017	arg1	regulators					2026:2035	the most ancestral immune regulators	2000:2035	the most ancestral immune regulators in vertebrates	2000:2050	Our composite results offer valuable clues to the origin and evolution of the TGF-β subfamily and imply that TGF-βs are among the most ancestral immune regulators in vertebrates.					
35309318	4	44	theme	Japanese	755:762	arg1	lamprey					764:770	the Japanese lamprey	751:770	the Japanese lamprey (Lampetra japonica)	751:790	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	44	theme	Japanese	755:762	arg1	japonica					782:789	Lampetra japonica	773:789	Lampetra japonica	773:789	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	0	45	theme	Growth	36:41	arg1	Family					65:70	Transforming Growth Factor-β (TGF-β) Gene Family	23:70	Transforming Growth Factor-β (TGF-β) Gene Family	23:70	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2.					
35309318	5	46	theme	lamprey	952:958	arg1	subfamily					966:974	the lamprey TGF-β subfamily	948:974	the lamprey TGF-β subfamily	948:974	The molecular evolutionary analyses reveal that the lamprey TGF-β subfamily contains two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively, but TGF-β1 is absent.					
35309318	2	47	theme	jawed	458:462	arg1	vertebrates					464:474	jawed vertebrates	458:474	jawed vertebrates	458:474	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	2	48	from	present	365:371	arg1	deuterostomes					376:388	deuterostomes	376:388	deuterostomes	376:388	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	7	49	from	leukocytes	1664:1673	arg1	lampreys					1678:1685	lampreys	1678:1685	lampreys	1678:1685	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	2	50	from	deuterostomes	376:388	arg1	present					365:371	present	365:371	present	365:371	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	2	50	from	deuterostomes	376:388	arg1	subfamily					340:348	The TGF-β subfamily	330:348	The TGF-β subfamily	330:348	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	2	51	theme	TGF-β	334:338	arg1	present					365:371	present	365:371	present	365:371	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	2	51	theme	TGF-β	334:338	arg1	subfamily					340:348	The TGF-β subfamily	330:348	The TGF-β subfamily	330:348	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	1	52	theme	cellular	238:245	arg1	behaviors					247:255	cellular behaviors	238:255	cellular behaviors	238:255	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	7	53	theme	TGF-β2	1415:1420	arg1	protein					1434:1440	the lamprey TGF-β2 recombinant protein	1403:1440	the lamprey TGF-β2 recombinant protein	1403:1440	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	6	54	theme	immune	1206:1211	arg1	response					1213:1220	the innate immune response	1195:1220	the innate immune response of the lamprey	1195:1235	The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli.					
35309318	7	55	from	proliferation	1479:1491	arg1	state					1578:1582	the quiescent and LPS-activated state	1546:1582	the quiescent and LPS-activated state	1546:1582	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	7	56	theme	assay	1384:1388	arg1	incorporation					1362:1374	The incorporation	1358:1374	The incorporation of BrdU assay	1358:1388	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	3	57	theme	evolutionary	490:501	arg1	processes					503:511	the evolutionary processes	486:511	the evolutionary processes of the TGF-β subfamily in vertebrates	486:549	However, the evolutionary processes of the TGF-β subfamily in vertebrates still lack sufficient elucidation.					
35309318	4	58	theme	Lampetra	773:780	arg1	lamprey					764:770	the Japanese lamprey	751:770	the Japanese lamprey (Lampetra japonica)	751:790	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	58	theme	Lampetra	773:780	arg1	japonica					782:789	Lampetra japonica	773:789	Lampetra japonica	773:789	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	6	59	theme	transcriptional	1092:1106	arg1	patterns					1119:1126	The transcriptional expression patterns	1088:1126	The transcriptional expression patterns	1088:1126	The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli.					
35309318	6	60	theme	expression	1300:1309	arg1	upregulation					1284:1295	a more rapid and significant upregulation	1255:1295	a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli	1255:1355	The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli.					
35309318	7	61	from	lampreys	1678:1685	arg1	proliferation					1623:1635	the proliferation	1619:1635	the proliferation of quiescent and activated leukocytes in lampreys	1619:1685	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	6	62	theme	rapid	1262:1266	arg1	upregulation					1284:1295	a more rapid and significant upregulation	1255:1295	a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli	1255:1355	The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli.					
35309318	3	63	theme	TGF-β	520:524	arg1	subfamily					526:534	the TGF-β subfamily	516:534	the TGF-β subfamily	516:534	However, the evolutionary processes of the TGF-β subfamily in vertebrates still lack sufficient elucidation.					
35309318	7	64	theme	LPS-activated	1564:1576	arg1	state					1578:1582	the quiescent and LPS-activated state	1546:1582	the quiescent and LPS-activated state	1546:1582	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	9	65	theme	valuable	1902:1909	arg1	clues					1911:1915	valuable clues	1902:1915	valuable clues to the origin and evolution of the TGF-β subfamily	1902:1966	Our composite results offer valuable clues to the origin and evolution of the TGF-β subfamily and imply that TGF-βs are among the most ancestral immune regulators in vertebrates.					
35309318	3	66	theme	sufficient	562:571	arg1	elucidation					573:583	sufficient elucidation	562:583	sufficient elucidation	562:583	However, the evolutionary processes of the TGF-β subfamily in vertebrates still lack sufficient elucidation.					
35309318	7	67	theme	quiescent	1550:1558	arg1	state					1578:1582	the quiescent and LPS-activated state	1546:1582	the quiescent and LPS-activated state	1546:1582	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	4	68	theme	TGF-β	605:609	arg1	homologs					611:618	the TGF-β homologs	601:618	the TGF-β homologs	601:618	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	5	69	from	TGF-β2	1023:1028	arg1	vertebrates					1039:1049	vertebrates	1039:1049	vertebrates	1039:1049	The molecular evolutionary analyses reveal that the lamprey TGF-β subfamily contains two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively, but TGF-β1 is absent.					
35309318	4	70	theme	reissner	667:674	arg1	lamprey					676:682	the reissner lamprey	663:682	the reissner lamprey (Lethenteron reissneri)	663:706	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	70	theme	reissner	667:674	arg1	reissneri					697:705	reissneri	697:705	reissneri	697:705	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	6	71	theme	lamprey	1229:1235	arg1	response					1213:1220	the innate immune response	1195:1220	the innate immune response of the lamprey	1195:1235	The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli.					
35309318	8	72	theme	activity	1713:1720	arg1	analysis					1722:1729	caspase-3/7 activity analysis	1701:1729	caspase-3/7 activity analysis	1701:1729	Furthermore, caspase-3/7 activity analysis indicates that the lamprey TGF-β2 protects SMB cells from apoptosis after serum deprivation, in contrast to promoting apoptosis of leukocytes.					
35309318	4	73	theme	jawless	833:839	arg1	vertebrates					841:851	jawless vertebrates	833:851	jawless vertebrates with a history of more than 350 million years	833:897	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	6	74	theme	lamprey	1142:1148	arg1	TGF-β2					1150:1155	the lamprey TGF-β2	1138:1155	the lamprey TGF-β2	1138:1155	The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli.					
35309318	0	75	theme	Gene	60:63	arg1	Family					65:70	Transforming Growth Factor-β (TGF-β) Gene Family	23:70	Transforming Growth Factor-β (TGF-β) Gene Family	23:70	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2.					
35309318	1	76	theme	transforming	131:142	arg1	TGF-βs					162:167	TGF-βs	162:167	TGF-βs	162:167	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	1	76	theme	transforming	131:142	arg1	factor-βs					151:159	The transforming growth factor-βs	127:159	The transforming growth factor-βs (TGF-βs)	127:168	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	1	76	theme	transforming	131:142	arg1	cytokines					190:198	multifunctional cytokines	174:198	multifunctional cytokines capable of regulating a wide range of cellular behaviors	174:255	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	4	77	theme	years	893:897	arg1	history					860:866	a history	858:866	a history of more than 350 million years	858:897	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	78	theme	genome-wide	642:652	arg1	representatives					814:828	the extant representatives	803:828	the extant representatives of jawless vertebrates with a history of more than 350 million years	803:897	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	78	theme	genome-wide	642:652	arg1	level					654:658	the genome-wide level	638:658	the genome-wide level	638:658	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	1	79	theme	immune	315:320	arg1	system					322:327	the immune system	311:327	the immune system	311:327	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	7	80	theme	leukocytes	1664:1673	arg1	proliferation					1623:1635	the proliferation	1619:1635	the proliferation of quiescent and activated leukocytes in lampreys	1619:1685	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	7	81	theme	body	1520:1523	arg1	cells					1525:1529	the supraneural myeloid body cells	1496:1529	the supraneural myeloid body cells (SMB cells)	1496:1541	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	7	81	theme	body	1520:1523	arg1	cells					1536:1540	SMB cells	1532:1540	SMB cells	1532:1540	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	8	82	theme	leukocytes	1862:1871	arg1	apoptosis					1849:1857	apoptosis	1849:1857	apoptosis of leukocytes	1849:1871	Furthermore, caspase-3/7 activity analysis indicates that the lamprey TGF-β2 protects SMB cells from apoptosis after serum deprivation, in contrast to promoting apoptosis of leukocytes.					
35309318	2	83	from	gene	413:416	arg1	invertebrates					421:433	invertebrates	421:433	invertebrates	421:433	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	2	83	from	gene	413:416	arg1	expands					391:397	expands	391:397	expands	391:397	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	9	84	theme	subfamily	1958:1966	arg1	origin					1924:1929	origin	1924:1929	origin	1924:1929	Our composite results offer valuable clues to the origin and evolution of the TGF-β subfamily and imply that TGF-βs are among the most ancestral immune regulators in vertebrates.					
35309318	9	84	theme	subfamily	1958:1966	arg1	evolution					1935:1943	evolution	1935:1943	evolution	1935:1943	Our composite results offer valuable clues to the origin and evolution of the TGF-β subfamily and imply that TGF-βs are among the most ancestral immune regulators in vertebrates.					
35309318	0	85	theme	Functional	80:89	arg1	Characterization					91:106	the Functional Characterization	76:106	the Functional Characterization of Lamprey TGF-β2	76:124	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2.					
35309318	7	86	theme	supraneural	1500:1510	arg1	cells					1525:1529	the supraneural myeloid body cells	1496:1529	the supraneural myeloid body cells (SMB cells)	1496:1541	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	7	86	theme	supraneural	1500:1510	arg1	cells					1536:1540	SMB cells	1532:1540	SMB cells	1532:1540	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
35309318	4	87	theme	sea	713:715	arg1	lamprey					717:723	the sea lamprey	709:723	the sea lamprey (Petromyzon marinus)	709:744	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	4	87	theme	sea	713:715	arg1	marinus					737:743	Petromyzon marinus	726:743	Petromyzon marinus	726:743	In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years.					
35309318	6	88	theme	significant	1272:1282	arg1	upregulation					1284:1295	a more rapid and significant upregulation	1255:1295	a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli	1255:1355	The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli.					
35309318	1	89	theme	capable	200:206	arg1	factor-βs					151:159	The transforming growth factor-βs	127:159	The transforming growth factor-βs (TGF-βs)	127:168	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	1	89	theme	capable	200:206	arg1	cytokines					190:198	multifunctional cytokines	174:198	multifunctional cytokines capable of regulating a wide range of cellular behaviors	174:255	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	5	90	from	ancestors	1010:1018	arg1	vertebrates					1039:1049	vertebrates	1039:1049	vertebrates	1039:1049	The molecular evolutionary analyses reveal that the lamprey TGF-β subfamily contains two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively, but TGF-β1 is absent.					
35309318	5	91	from	vertebrates	1039:1049	arg1	ancestors					1010:1018	ancestors	1010:1018	ancestors of TGF-β2 and 3 in vertebrates, respectively	1010:1063	The molecular evolutionary analyses reveal that the lamprey TGF-β subfamily contains two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively, but TGF-β1 is absent.					
35309318	2	92	theme	single	406:411	arg1	gene					413:416	a single gene	404:416	a single gene in invertebrates to multiple members in jawed vertebrates	404:474	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	0	93	theme	Transforming	23:34	arg1	Family					65:70	Transforming Growth Factor-β (TGF-β) Gene Family	23:70	Transforming Growth Factor-β (TGF-β) Gene Family	23:70	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2.					
35309318	1	94	theme	key	268:270	arg1	role					272:275	a key role	266:275	a key role	266:275	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	8	95	theme	SMB	1774:1776	arg1	cells					1778:1782	SMB cells	1774:1782	SMB cells	1774:1782	Furthermore, caspase-3/7 activity analysis indicates that the lamprey TGF-β2 protects SMB cells from apoptosis after serum deprivation, in contrast to promoting apoptosis of leukocytes.					
35309318	0	96	theme	Factor-β	43:50	arg1	Family					65:70	Transforming Growth Factor-β (TGF-β) Gene Family	23:70	Transforming Growth Factor-β (TGF-β) Gene Family	23:70	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2.					
35309318	9	97	theme	immune	2019:2024	arg1	regulators					2026:2035	the most ancestral immune regulators	2000:2035	the most ancestral immune regulators in vertebrates	2000:2050	Our composite results offer valuable clues to the origin and evolution of the TGF-β subfamily and imply that TGF-βs are among the most ancestral immune regulators in vertebrates.					
35309318	0	98	theme	TGF-β	53:57	arg1	Family					65:70	Transforming Growth Factor-β (TGF-β) Gene Family	23:70	Transforming Growth Factor-β (TGF-β) Gene Family	23:70	Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2.					
35309318	5	99	theme	TGF-β	960:964	arg1	subfamily					966:974	the lamprey TGF-β subfamily	948:974	the lamprey TGF-β subfamily	948:974	The molecular evolutionary analyses reveal that the lamprey TGF-β subfamily contains two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively, but TGF-β1 is absent.					
35309318	5	100	contain	contains	976:983	arg2	members					989:995	two members	985:995	two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively	985:1063	The molecular evolutionary analyses reveal that the lamprey TGF-β subfamily contains two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively, but TGF-β1 is absent.					
35309318	5	100	contain	contains	976:983	arg1	subfamily					966:974	the lamprey TGF-β subfamily	948:974	the lamprey TGF-β subfamily	948:974	The molecular evolutionary analyses reveal that the lamprey TGF-β subfamily contains two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively, but TGF-β1 is absent.					
35309318	2	101	attach	present	365:371	arg1	deuterostomes					376:388	deuterostomes	376:388	deuterostomes	376:388	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	2	101	attach	present	365:371	arg2	subfamily					340:348	The TGF-β subfamily	330:348	The TGF-β subfamily	330:348	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	2	101	attach	present	365:371	arg2	present					365:371	present	365:371	present	365:371	The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates.					
35309318	5	102	theme	evolutionary	914:925	arg1	analyses					927:934	The molecular evolutionary analyses	900:934	The molecular evolutionary analyses	900:934	The molecular evolutionary analyses reveal that the lamprey TGF-β subfamily contains two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively, but TGF-β1 is absent.					
35309318	1	103	theme	wide	224:227	arg1	range					229:233	a wide range	222:233	a wide range of cellular behaviors	222:255	The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system.					
35309318	7	104	theme	bipolar	1453:1459	arg1	regulation					1461:1470	the bipolar regulation	1449:1470	the bipolar regulation	1449:1470	The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys.					
37097729	4	0	theme	structures	731:740	arg1	formation					684:692	stabilized formation	673:692	stabilized formation of long-range G-quadruplex secondary structures	673:740	Combining poly(trehalose-co-guanosine) (pTreGuo) glycopolymers with free guanosine (fGuo) generates shear thinning hydrogels through stabilized formation of long-range G-quadruplex secondary structures.					
37097729	3	1	theme	CNS	528:530	arg1	injury					532:537	CNS injury	528:537	CNS injury	528:537	In this work, glycopolymer-based supramolecular hydrogels are synthesized to direct adaptive glia repair after CNS injury.					
37097729	4	2	theme	free	608:611	arg1	guanosine					613:621	free guanosine	608:621	free guanosine (fGuo)	608:628	Combining poly(trehalose-co-guanosine) (pTreGuo) glycopolymers with free guanosine (fGuo) generates shear thinning hydrogels through stabilized formation of long-range G-quadruplex secondary structures.					
37097729	4	2	theme	free	608:611	arg1	fGuo					624:627	fGuo	624:627	fGuo	624:627	Combining poly(trehalose-co-guanosine) (pTreGuo) glycopolymers with free guanosine (fGuo) generates shear thinning hydrogels through stabilized formation of long-range G-quadruplex secondary structures.					
37097729	6	3	theme	cell	1003:1006	arg1	infiltration					1008:1019	minimal stromal cell infiltration	987:1019	minimal stromal cell infiltration	987:1019	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
37097729	6	4	theme	stromal	995:1001	arg1	infiltration					1008:1019	minimal stromal cell infiltration	987:1019	minimal stromal cell infiltration	987:1019	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
37097729	9	5	theme	repair	1570:1575	arg1	mechanisms					1577:1586	endogenous glia repair mechanisms	1554:1586	endogenous glia repair mechanisms	1554:1586	These results support the use of pTreGuo hydrogels as part of neural regeneration strategies to activate endogenous glia repair mechanisms.					
37097729	2	6	dep	repair	290:295	arg1	create					300:305	create	300:305	to create a more permissive environment for regeneration	297:352	Scar-free repair to create a more permissive environment for regeneration requires altering the natural injury responses of glial cells.					
37097729	6	7	theme	methyl	1092:1097	arg1	material					1122:1129	a bioinert methyl cellulose benchmarking material	1081:1129	a bioinert methyl cellulose benchmarking material	1081:1129	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
37097729	8	8	theme	hydrogels	1274:1282	arg1	Injections					1252:1261	Injections	1252:1261	Injections of pTreGuo hydrogels into ischemic stroke	1252:1303	Injections of pTreGuo hydrogels into ischemic stroke alter the natural responses of glial cells after injury to reduce the size of lesions and increase axon regrowth into lesion core environments.					
37097729	6	9	theme	minimal	987:993	arg1	infiltration					1008:1019	minimal stromal cell infiltration	987:1019	minimal stromal cell infiltration	987:1019	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
37097729	7	10	theme	hydrogel	1225:1232	arg1	bulk					1234:1237	the hydrogel bulk	1221:1237	the hydrogel bulk	1221:1237	pTreGuo hydrogels alter astrocyte borders and recruit microglia to infiltrate and resorb the hydrogel bulk over 7 days.					
37097729	9	11	theme	endogenous	1554:1563	arg1	mechanisms					1577:1586	endogenous glia repair mechanisms	1554:1586	endogenous glia repair mechanisms	1554:1586	These results support the use of pTreGuo hydrogels as part of neural regeneration strategies to activate endogenous glia repair mechanisms.					
37097729	1	12	theme	neurological	257:268	arg1	function					270:277	no neurological function	254:277	no neurological function	254:277	Neural tissue damaged after central nervous system (CNS) injury does not naturally regenerate but is instead replaced by non-neural fibrotic scar tissue that serves no neurological function.					
37097729	8	13	theme	pTreGuo	1266:1272	arg1	hydrogels					1274:1282	pTreGuo hydrogels	1266:1282	pTreGuo hydrogels	1266:1282	Injections of pTreGuo hydrogels into ischemic stroke alter the natural responses of glial cells after injury to reduce the size of lesions and increase axon regrowth into lesion core environments.					
37097729	7	14	theme	pTreGuo	1132:1138	arg1	hydrogels					1140:1148	pTreGuo hydrogels	1132:1148	pTreGuo hydrogels	1132:1148	pTreGuo hydrogels alter astrocyte borders and recruit microglia to infiltrate and resorb the hydrogel bulk over 7 days.					
37097729	6	15	theme	benchmarking	1109:1120	arg1	material					1122:1129	a bioinert methyl cellulose benchmarking material	1081:1129	a bioinert methyl cellulose benchmarking material	1081:1129	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
37097729	5	16	theme	mechanical	797:806	arg1	properties					808:817	mechanical properties	797:817	mechanical properties	797:817	Hydrogels with smooth or granular microstructures and mechanical properties spanning three orders of magnitude are produced through facile control of pTreGuo hydrogel composition.					
37097729	0	17	from	Responses	64:72	arg1	Injury					81:86	CNS Injury	77:86	CNS Injury	77:86	Trehalose-Guanosine Glycopolymer Hydrogels Direct Adaptive Glia Responses in CNS Injury.					
37097729	8	18	theme	ischemic	1289:1296	arg1	stroke					1298:1303	ischemic stroke	1289:1303	ischemic stroke	1289:1303	Injections of pTreGuo hydrogels into ischemic stroke alter the natural responses of glial cells after injury to reduce the size of lesions and increase axon regrowth into lesion core environments.					
37097729	5	19	theme	composition	910:920	arg1	control					882:888	facile control	875:888	facile control of pTreGuo hydrogel composition	875:920	Hydrogels with smooth or granular microstructures and mechanical properties spanning three orders of magnitude are produced through facile control of pTreGuo hydrogel composition.					
37097729	2	20	theme	Scar-free	280:288	arg1	repair					290:295	Scar-free repair	280:295	Scar-free repair to create a more permissive environment for regeneration	280:352	Scar-free repair to create a more permissive environment for regeneration requires altering the natural injury responses of glial cells.					
37097729	9	21	theme	glia	1565:1568	arg1	mechanisms					1577:1586	endogenous glia repair mechanisms	1554:1586	endogenous glia repair mechanisms	1554:1586	These results support the use of pTreGuo hydrogels as part of neural regeneration strategies to activate endogenous glia repair mechanisms.					
37097729	2	22	theme	cells	410:414	arg1	responses					391:399	the natural injury responses	372:399	the natural injury responses of glial cells	372:414	Scar-free repair to create a more permissive environment for regeneration requires altering the natural injury responses of glial cells.					
37097729	5	23	theme	facile	875:880	arg1	control					882:888	facile control	875:888	facile control of pTreGuo hydrogel composition	875:920	Hydrogels with smooth or granular microstructures and mechanical properties spanning three orders of magnitude are produced through facile control of pTreGuo hydrogel composition.					
37097729	5	24	theme	magnitude	844:852	arg1	orders					834:839	three orders	828:839	three orders of magnitude	828:852	Hydrogels with smooth or granular microstructures and mechanical properties spanning three orders of magnitude are produced through facile control of pTreGuo hydrogel composition.					
37097729	1	25	theme	Neural	89:94	arg1	tissue					96:101	Neural tissue	89:101	Neural tissue damaged after central nervous system (CNS) injury	89:151	Neural tissue damaged after central nervous system (CNS) injury does not naturally regenerate but is instead replaced by non-neural fibrotic scar tissue that serves no neurological function.					
37097729	2	26	theme	glial	404:408	arg1	cells					410:414	glial cells	404:414	glial cells	404:414	Scar-free repair to create a more permissive environment for regeneration requires altering the natural injury responses of glial cells.					
37097729	0	27	theme	Glycopolymer	20:31	arg1	Hydrogels					33:41	Trehalose-Guanosine Glycopolymer Hydrogels	0:41	Trehalose-Guanosine Glycopolymer Hydrogels	0:41	Trehalose-Guanosine Glycopolymer Hydrogels Direct Adaptive Glia Responses in CNS Injury.					
37097729	6	28	theme	healthy	959:965	arg1	brain					973:977	healthy mouse brain	959:977	healthy mouse brain	959:977	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
37097729	2	29	theme	permissive	314:323	arg1	environment					325:335	a more permissive environment	307:335	a more permissive environment for regeneration	307:352	Scar-free repair to create a more permissive environment for regeneration requires altering the natural injury responses of glial cells.					
37097729	4	30	theme	G-quadruplex	708:719	arg1	structures					731:740	long-range G-quadruplex secondary structures	697:740	long-range G-quadruplex secondary structures	697:740	Combining poly(trehalose-co-guanosine) (pTreGuo) glycopolymers with free guanosine (fGuo) generates shear thinning hydrogels through stabilized formation of long-range G-quadruplex secondary structures.					
37097729	0	31	theme	Trehalose-Guanosine	0:18	arg1	Hydrogels					33:41	Trehalose-Guanosine Glycopolymer Hydrogels	0:41	Trehalose-Guanosine Glycopolymer Hydrogels	0:41	Trehalose-Guanosine Glycopolymer Hydrogels Direct Adaptive Glia Responses in CNS Injury.					
37097729	6	32	theme	cellulose	1099:1107	arg1	material					1122:1129	a bioinert methyl cellulose benchmarking material	1081:1129	a bioinert methyl cellulose benchmarking material	1081:1129	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
37097729	3	33	theme	glycopolymer-based	431:448	arg1	hydrogels					465:473	glycopolymer-based supramolecular hydrogels	431:473	glycopolymer-based supramolecular hydrogels	431:473	In this work, glycopolymer-based supramolecular hydrogels are synthesized to direct adaptive glia repair after CNS injury.					
37097729	8	34	theme	natural	1315:1321	arg1	responses					1323:1331	the natural responses	1311:1331	the natural responses of glial cells after injury	1311:1359	Injections of pTreGuo hydrogels into ischemic stroke alter the natural responses of glial cells after injury to reduce the size of lesions and increase axon regrowth into lesion core environments.					
37097729	8	35	theme	axon	1404:1407	arg1	regrowth					1409:1416	axon regrowth	1404:1416	axon regrowth into lesion core environments	1404:1446	Injections of pTreGuo hydrogels into ischemic stroke alter the natural responses of glial cells after injury to reduce the size of lesions and increase axon regrowth into lesion core environments.					
37097729	8	36	theme	core	1430:1433	arg1	environments					1435:1446	lesion core environments	1423:1446	lesion core environments	1423:1446	Injections of pTreGuo hydrogels into ischemic stroke alter the natural responses of glial cells after injury to reduce the size of lesions and increase axon regrowth into lesion core environments.					
37097729	5	37	theme	hydrogel	901:908	arg1	composition					910:920	pTreGuo hydrogel composition	893:920	pTreGuo hydrogel composition	893:920	Hydrogels with smooth or granular microstructures and mechanical properties spanning three orders of magnitude are produced through facile control of pTreGuo hydrogel composition.					
37097729	5	38	theme	pTreGuo	893:899	arg1	composition					910:920	pTreGuo hydrogel composition	893:920	pTreGuo hydrogel composition	893:920	Hydrogels with smooth or granular microstructures and mechanical properties spanning three orders of magnitude are produced through facile control of pTreGuo hydrogel composition.					
37097729	5	39	theme	smooth	758:763	arg1	microstructures					777:791	smooth or granular microstructures	758:791	smooth or granular microstructures	758:791	Hydrogels with smooth or granular microstructures and mechanical properties spanning three orders of magnitude are produced through facile control of pTreGuo hydrogel composition.					
37097729	1	40	theme	central	117:123	arg1	CNS					141:143	CNS	141:143	CNS	141:143	Neural tissue damaged after central nervous system (CNS) injury does not naturally regenerate but is instead replaced by non-neural fibrotic scar tissue that serves no neurological function.					
37097729	1	40	theme	central	117:123	arg1	system					133:138	central nervous system	117:138	central nervous system (CNS) injury	117:151	Neural tissue damaged after central nervous system (CNS) injury does not naturally regenerate but is instead replaced by non-neural fibrotic scar tissue that serves no neurological function.					
37097729	4	41	theme	thinning	646:653	arg1	hydrogels					655:663	shear thinning hydrogels	640:663	shear thinning hydrogels	640:663	Combining poly(trehalose-co-guanosine) (pTreGuo) glycopolymers with free guanosine (fGuo) generates shear thinning hydrogels through stabilized formation of long-range G-quadruplex secondary structures.					
37097729	0	42	theme	Glia	59:62	arg1	Responses					64:72	Adaptive Glia Responses	50:72	Adaptive Glia Responses in CNS Injury	50:86	Trehalose-Guanosine Glycopolymer Hydrogels Direct Adaptive Glia Responses in CNS Injury.					
37097729	6	43	theme	hydrogels	944:952	arg1	Injection					923:931	Injection	923:931	Injection of pTreGuo hydrogels into healthy mouse brain	923:977	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
37097729	4	44	theme	long-range	697:706	arg1	structures					731:740	long-range G-quadruplex secondary structures	697:740	long-range G-quadruplex secondary structures	697:740	Combining poly(trehalose-co-guanosine) (pTreGuo) glycopolymers with free guanosine (fGuo) generates shear thinning hydrogels through stabilized formation of long-range G-quadruplex secondary structures.					
37097729	4	45	theme	stabilized	673:682	arg1	formation					684:692	stabilized formation	673:692	stabilized formation of long-range G-quadruplex secondary structures	673:740	Combining poly(trehalose-co-guanosine) (pTreGuo) glycopolymers with free guanosine (fGuo) generates shear thinning hydrogels through stabilized formation of long-range G-quadruplex secondary structures.					
37097729	0	46	theme	Adaptive	50:57	arg1	Responses					64:72	Adaptive Glia Responses	50:72	Adaptive Glia Responses in CNS Injury	50:86	Trehalose-Guanosine Glycopolymer Hydrogels Direct Adaptive Glia Responses in CNS Injury.					
37097729	5	47	theme	granular	768:775	arg1	microstructures					777:791	smooth or granular microstructures	758:791	smooth or granular microstructures	758:791	Hydrogels with smooth or granular microstructures and mechanical properties spanning three orders of magnitude are produced through facile control of pTreGuo hydrogel composition.					
37097729	6	48	theme	pTreGuo	936:942	arg1	hydrogels					944:952	pTreGuo hydrogels	936:952	pTreGuo hydrogels	936:952	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
37097729	9	49	theme	neural	1511:1516	arg1	regeneration					1518:1529	neural regeneration	1511:1529	neural regeneration	1511:1529	These results support the use of pTreGuo hydrogels as part of neural regeneration strategies to activate endogenous glia repair mechanisms.					
37097729	1	50	theme	non-neural	210:219	arg1	tissue					235:240	non-neural fibrotic scar tissue	210:240	non-neural fibrotic scar tissue that serves no neurological function	210:277	Neural tissue damaged after central nervous system (CNS) injury does not naturally regenerate but is instead replaced by non-neural fibrotic scar tissue that serves no neurological function.					
37097729	8	51	theme	cells	1342:1346	arg1	responses					1323:1331	the natural responses	1311:1331	the natural responses of glial cells after injury	1311:1359	Injections of pTreGuo hydrogels into ischemic stroke alter the natural responses of glial cells after injury to reduce the size of lesions and increase axon regrowth into lesion core environments.					
37097729	4	52	theme	poly	550:553	arg1	glycopolymers					589:601	poly(trehalose-co-guanosine) (pTreGuo) glycopolymers	550:601	poly(trehalose-co-guanosine) (pTreGuo) glycopolymers	550:601	Combining poly(trehalose-co-guanosine) (pTreGuo) glycopolymers with free guanosine (fGuo) generates shear thinning hydrogels through stabilized formation of long-range G-quadruplex secondary structures.					
37097729	7	53	theme	astrocyte	1156:1164	arg1	borders					1166:1172	astrocyte borders	1156:1172	astrocyte borders	1156:1172	pTreGuo hydrogels alter astrocyte borders and recruit microglia to infiltrate and resorb the hydrogel bulk over 7 days.					
37097729	3	54	theme	supramolecular	450:463	arg1	hydrogels					465:473	glycopolymer-based supramolecular hydrogels	431:473	glycopolymer-based supramolecular hydrogels	431:473	In this work, glycopolymer-based supramolecular hydrogels are synthesized to direct adaptive glia repair after CNS injury.					
37097729	6	55	theme	mouse	967:971	arg1	brain					973:977	healthy mouse brain	959:977	healthy mouse brain	959:977	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
37097729	1	56	theme	nervous	125:131	arg1	CNS					141:143	CNS	141:143	CNS	141:143	Neural tissue damaged after central nervous system (CNS) injury does not naturally regenerate but is instead replaced by non-neural fibrotic scar tissue that serves no neurological function.					
37097729	1	56	theme	nervous	125:131	arg1	system					133:138	central nervous system	117:138	central nervous system (CNS) injury	117:151	Neural tissue damaged after central nervous system (CNS) injury does not naturally regenerate but is instead replaced by non-neural fibrotic scar tissue that serves no neurological function.					
37097729	8	57	theme	glial	1336:1340	arg1	cells					1342:1346	glial cells	1336:1346	glial cells	1336:1346	Injections of pTreGuo hydrogels into ischemic stroke alter the natural responses of glial cells after injury to reduce the size of lesions and increase axon regrowth into lesion core environments.					
37097729	6	58	theme	bioinert	1083:1090	arg1	material					1122:1129	a bioinert methyl cellulose benchmarking material	1081:1129	a bioinert methyl cellulose benchmarking material	1081:1129	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
37097729	1	59	theme	system	133:138	arg1	injury					146:151	central nervous system (CNS) injury	117:151	central nervous system (CNS) injury	117:151	Neural tissue damaged after central nervous system (CNS) injury does not naturally regenerate but is instead replaced by non-neural fibrotic scar tissue that serves no neurological function.					
37097729	9	60	theme	pTreGuo	1482:1488	arg1	hydrogels					1490:1498	pTreGuo hydrogels	1482:1498	pTreGuo hydrogels	1482:1498	These results support the use of pTreGuo hydrogels as part of neural regeneration strategies to activate endogenous glia repair mechanisms.					
37097729	8	61	theme	lesions	1383:1389	arg1	size					1375:1378	the size	1371:1378	the size of lesions	1371:1389	Injections of pTreGuo hydrogels into ischemic stroke alter the natural responses of glial cells after injury to reduce the size of lesions and increase axon regrowth into lesion core environments.					
37097729	4	62	theme	secondary	721:729	arg1	structures					731:740	long-range G-quadruplex secondary structures	697:740	long-range G-quadruplex secondary structures	697:740	Combining poly(trehalose-co-guanosine) (pTreGuo) glycopolymers with free guanosine (fGuo) generates shear thinning hydrogels through stabilized formation of long-range G-quadruplex secondary structures.					
37097729	3	63	theme	adaptive	501:508	arg1	repair					515:520	adaptive glia repair	501:520	adaptive glia repair	501:520	In this work, glycopolymer-based supramolecular hydrogels are synthesized to direct adaptive glia repair after CNS injury.					
37097729	9	64	theme	hydrogels	1490:1498	arg1	use					1475:1477	the use	1471:1477	the use of pTreGuo hydrogels as part of neural regeneration strategies to activate endogenous glia repair mechanisms	1471:1586	These results support the use of pTreGuo hydrogels as part of neural regeneration strategies to activate endogenous glia repair mechanisms.					
37097729	1	65	theme	fibrotic	221:228	arg1	tissue					235:240	non-neural fibrotic scar tissue	210:240	non-neural fibrotic scar tissue that serves no neurological function	210:277	Neural tissue damaged after central nervous system (CNS) injury does not naturally regenerate but is instead replaced by non-neural fibrotic scar tissue that serves no neurological function.					
37097729	9	66	theme	regeneration	1518:1529	arg1	part					1503:1506	part	1503:1506	part of neural regeneration strategies to activate endogenous glia repair mechanisms	1503:1586	These results support the use of pTreGuo hydrogels as part of neural regeneration strategies to activate endogenous glia repair mechanisms.					
37097729	8	67	theme	lesion	1423:1428	arg1	environments					1435:1446	lesion core environments	1423:1446	lesion core environments	1423:1446	Injections of pTreGuo hydrogels into ischemic stroke alter the natural responses of glial cells after injury to reduce the size of lesions and increase axon regrowth into lesion core environments.					
37097729	3	68	theme	glia	510:513	arg1	repair					515:520	adaptive glia repair	501:520	adaptive glia repair	501:520	In this work, glycopolymer-based supramolecular hydrogels are synthesized to direct adaptive glia repair after CNS injury.					
37097729	1	69	theme	scar	230:233	arg1	tissue					235:240	non-neural fibrotic scar tissue	210:240	non-neural fibrotic scar tissue that serves no neurological function	210:277	Neural tissue damaged after central nervous system (CNS) injury does not naturally regenerate but is instead replaced by non-neural fibrotic scar tissue that serves no neurological function.					
37097729	2	70	theme	injury	384:389	arg1	responses					391:399	the natural injury responses	372:399	the natural injury responses of glial cells	372:414	Scar-free repair to create a more permissive environment for regeneration requires altering the natural injury responses of glial cells.					
37097729	5	71	with	Hydrogels	743:751	arg1	microstructures					777:791	smooth or granular microstructures	758:791	smooth or granular microstructures	758:791	Hydrogels with smooth or granular microstructures and mechanical properties spanning three orders of magnitude are produced through facile control of pTreGuo hydrogel composition.					
37097729	5	71	with	Hydrogels	743:751	arg1	properties					808:817	mechanical properties	797:817	mechanical properties	797:817	Hydrogels with smooth or granular microstructures and mechanical properties spanning three orders of magnitude are produced through facile control of pTreGuo hydrogel composition.					
37097729	4	72	theme	shear	640:644	arg1	hydrogels					655:663	shear thinning hydrogels	640:663	shear thinning hydrogels	640:663	Combining poly(trehalose-co-guanosine) (pTreGuo) glycopolymers with free guanosine (fGuo) generates shear thinning hydrogels through stabilized formation of long-range G-quadruplex secondary structures.					
37097729	0	73	theme	CNS	77:79	arg1	Injury					81:86	CNS Injury	77:86	CNS Injury	77:86	Trehalose-Guanosine Glycopolymer Hydrogels Direct Adaptive Glia Responses in CNS Injury.					
37097729	6	74	link	derived	1038:1044	arg1	inflammation					1046:1057	peripherally derived inflammation	1025:1057	peripherally derived inflammation	1025:1057	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
37097729	2	75	theme	natural	376:382	arg1	responses					391:399	the natural injury responses	372:399	the natural injury responses of glial cells	372:414	Scar-free repair to create a more permissive environment for regeneration requires altering the natural injury responses of glial cells.					
37097729	6	76	theme	derived	1038:1044	arg1	inflammation					1046:1057	peripherally derived inflammation	1025:1057	peripherally derived inflammation	1025:1057	Injection of pTreGuo hydrogels into healthy mouse brain elicits minimal stromal cell infiltration and peripherally derived inflammation that is comparable to a bioinert methyl cellulose benchmarking material.					
36328080	7	0	theme	higher	1111:1116	arg1	pKas					1118:1121	higher pKas	1111:1121	higher pKas of the histidine residues in SDS than in DPC	1111:1166	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	3	1	theme	selectivity	514:524	arg1	action					478:483	action	478:483	action of Of-Pis1 and its potential selectivity	478:524	To better understand the mechanism of action of Of-Pis1 and its potential selectivity, using NMR and CD spectroscopies, we studied the interaction with eukaryotic and procaryotic membranes and membrane models.					
36328080	4	2	used	used	730:733	arg2	micelles					716:723	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles	650:723	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles	650:723	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	11	3	theme	active	1670:1675	arg1	AMP					1677:1679	this broadly active AMP	1657:1679	this broadly active AMP	1657:1679	Overall, these data clearly show the selective interaction of this broadly active AMP with bacterial over eukaryotic membranes.					
36328080	4	4	used	used	819:822	arg2	DPC					810:812	DPC	810:812	DPC	810:812	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	4	used	used	819:822	arg2	phosphocholine					794:807	zwitterionic dodecyl phosphocholine	773:807	zwitterionic dodecyl phosphocholine (DPC)	773:813	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	4	used	used	819:822	arg2	surrogate					847:855	eukaryotic membrane surrogate	827:855	eukaryotic membrane surrogate	827:855	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	5	theme	membrane	838:845	arg1	surrogate					847:855	eukaryotic membrane surrogate	827:855	eukaryotic membrane surrogate	827:855	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	5	theme	membrane	838:845	arg1	phosphocholine					794:807	zwitterionic dodecyl phosphocholine	773:807	zwitterionic dodecyl phosphocholine (DPC)	773:813	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	10	6	with	interaction	1536:1546	arg1	bacterial					1553:1561	bacterial	1553:1561	bacterial	1553:1561	Furthermore, ligand-based NMR experiments support a tighter and more specific interaction with bacterial than with eukaryotic membranes.					
36328080	4	7	theme	sulfate	673:679	arg1	micelles					716:723	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles	650:723	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles	650:723	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	7	8	theme	Slower	1083:1088	arg1	exchange					1098:1105	Slower solvent exchange	1083:1105	Slower solvent exchange	1083:1105	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	6	9	from	face	1042:1045	arg1	contact					1056:1062	close contact	1050:1062	close contact with the micelles	1050:1080	In DPC and SDS instead, the N-terminal region of Of-Pis1 forms an amphipathic α-helix with the non-polar face in close contact with the micelles.					
36328080	2	10	theme	acid	289:292	arg1	peptide					294:300	This rich in histidines and glycines 24-amino acid peptide	243:300	This rich in histidines and glycines 24-amino acid peptide	243:300	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	11	11	with	interaction	1642:1652	arg1	bacterial					1686:1694	bacterial	1686:1694	bacterial	1686:1694	Overall, these data clearly show the selective interaction of this broadly active AMP with bacterial over eukaryotic membranes.					
36328080	4	12	theme	sodium	658:663	arg1	sulfate					673:679	Anionic sodium dodecyl sulfate	650:679	Anionic sodium dodecyl sulfate (SDS)	650:685	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	12	theme	sodium	658:663	arg1	SDS					682:684	SDS	682:684	SDS	682:684	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	1	13	dep	Oplegnathus	219:229	arg1	fasciatus					231:239	Oplegnathus fasciatus	219:239	Oplegnathus fasciatus	219:239	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	8	14	theme	perturbs	1266:1273	arg1	micelles					1279:1286	perturbs LPS micelles	1266:1286	perturbs LPS micelles	1266:1286	Of-Pis1 also binds tightly and structurally perturbs LPS micelles.					
36328080	5	15	theme	random	911:916	arg1	conformation					923:934	a flexible random coil conformation	900:934	a flexible random coil conformation	900:934	In an aqueous environment, Of-Pis1 adopts a flexible random coil conformation.					
36328080	3	16	theme	membrane	633:640	arg1	models					642:647	membrane models	633:647	membrane models	633:647	To better understand the mechanism of action of Of-Pis1 and its potential selectivity, using NMR and CD spectroscopies, we studied the interaction with eukaryotic and procaryotic membranes and membrane models.					
36328080	1	17	theme	potent	139:144	arg1	AMP					178:180	AMP	178:180	AMP	178:180	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	1	17	theme	potent	139:144	arg1	peptide					169:175	a potent piscidin antimicrobial peptide	137:175	a potent piscidin antimicrobial peptide (AMP)	137:181	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	1	17	theme	potent	139:144	arg1	Of-Pis1					126:132	Of-Pis1	126:132	Of-Pis1	126:132	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	12	18	theme	conformational	1727:1740	arg1	information					1742:1752	The conformational information	1723:1752	The conformational information	1723:1752	The conformational information is discussed in terms of Of-Pis1 amino acid sequence and composition to provide insights useful to design more potent and selective AMPs.					
36328080	6	19	theme	amphipathic	1003:1013	arg1	α-helix					1015:1021	an amphipathic α-helix	1000:1021	an amphipathic α-helix	1000:1021	In DPC and SDS instead, the N-terminal region of Of-Pis1 forms an amphipathic α-helix with the non-polar face in close contact with the micelles.					
36328080	0	20	theme	histidine-rich	56:69	arg1	Of-Pis1					102:108	the histidine-rich piscidin antimicrobial peptide Of-Pis1	52:108	the histidine-rich piscidin antimicrobial peptide Of-Pis1	52:108	Structural insights on the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes.					
36328080	3	21	theme	CD	541:542	arg1	spectroscopies					544:557	NMR and CD spectroscopies	533:557	spectroscopies	544:557	To better understand the mechanism of action of Of-Pis1 and its potential selectivity, using NMR and CD spectroscopies, we studied the interaction with eukaryotic and procaryotic membranes and membrane models.					
36328080	1	22	theme	antimicrobial	155:167	arg1	AMP					178:180	AMP	178:180	AMP	178:180	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	1	22	theme	antimicrobial	155:167	arg1	peptide					169:175	a potent piscidin antimicrobial peptide	137:175	a potent piscidin antimicrobial peptide (AMP)	137:181	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	1	22	theme	antimicrobial	155:167	arg1	Of-Pis1					126:132	Of-Pis1	126:132	Of-Pis1	126:132	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	0	23	theme	antimicrobial	80:92	arg1	Of-Pis1					102:108	the histidine-rich piscidin antimicrobial peptide Of-Pis1	52:108	the histidine-rich piscidin antimicrobial peptide Of-Pis1	52:108	Structural insights on the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes.					
36328080	2	24	theme	rich	248:251	arg1	peptide					294:300	This rich in histidines and glycines 24-amino acid peptide	243:300	This rich in histidines and glycines 24-amino acid peptide	243:300	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	0	25	theme	Of-Pis1	102:108	arg1	interaction					37:47	the selective interaction	23:47	the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes	23:123	Structural insights on the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes.					
36328080	6	26	theme	N-terminal	965:974	arg1	region					976:981	the N-terminal region	961:981	the N-terminal region of Of-Pis1	961:992	In DPC and SDS instead, the N-terminal region of Of-Pis1 forms an amphipathic α-helix with the non-polar face in close contact with the micelles.					
36328080	2	27	theme	low	426:428	arg1	toxicity					430:437	low toxicity	426:437	low toxicity	426:437	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	4	28	theme	zwitterionic	773:784	arg1	surrogate					847:855	eukaryotic membrane surrogate	827:855	eukaryotic membrane surrogate	827:855	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	28	theme	zwitterionic	773:784	arg1	DPC					810:812	DPC	810:812	DPC	810:812	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	28	theme	zwitterionic	773:784	arg1	phosphocholine					794:807	zwitterionic dodecyl phosphocholine	773:807	zwitterionic dodecyl phosphocholine (DPC)	773:813	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	1	29	dep	peptide	169:175	arg1	Oplegnathus					219:229	Oplegnathus	219:229	Oplegnathus	219:229	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	0	30	theme	Structural	0:9	arg1	insights					11:18	Structural insights	0:18	Structural insights on the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes.	0:124	Structural insights on the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes.					
36328080	12	31	theme	Of-Pis1	1779:1785	arg1	sequence					1798:1805	Of-Pis1 amino acid sequence	1779:1805	Of-Pis1 amino acid sequence	1779:1805	The conformational information is discussed in terms of Of-Pis1 amino acid sequence and composition to provide insights useful to design more potent and selective AMPs.					
36328080	4	32	theme	procaryotic	744:754	arg1	membranes					756:764	procaryotic membranes	744:764	procaryotic membranes	744:764	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	6	33	theme	non-polar	1032:1040	arg1	face					1042:1045	the non-polar face	1028:1045	the non-polar face in close contact with the micelles	1028:1080	In DPC and SDS instead, the N-terminal region of Of-Pis1 forms an amphipathic α-helix with the non-polar face in close contact with the micelles.					
36328080	10	34	theme	ligand-based	1471:1482	arg1	experiments					1488:1498	ligand-based NMR experiments	1471:1498	ligand-based NMR experiments	1471:1498	Furthermore, ligand-based NMR experiments support a tighter and more specific interaction with bacterial than with eukaryotic membranes.					
36328080	11	35	theme	eukaryotic	1701:1710	arg1	membranes					1712:1720	eukaryotic membranes	1701:1720	eukaryotic membranes	1701:1720	Overall, these data clearly show the selective interaction of this broadly active AMP with bacterial over eukaryotic membranes.					
36328080	0	36	theme	selective	27:35	arg1	interaction					37:47	the selective interaction	23:47	the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes	23:123	Structural insights on the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes.					
36328080	9	37	theme	mammalian	1338:1346	arg1	membranes					1353:1361	mammalian cell membranes	1338:1361	mammalian cell membranes	1338:1361	Of-Pis1 interacts with both Escherichia coli and mammalian cell membranes, but only in the presence of Escherichia coli membranes it populates the helical conformation.					
36328080	7	38	theme	histidine	1130:1138	arg1	residues					1140:1147	the histidine residues	1126:1147	the histidine residues in SDS	1126:1154	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	7	39	from	exchange	1098:1105	arg1	SDS					1152:1154	SDS	1152:1154	SDS	1152:1154	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	7	39	from	exchange	1098:1105	arg1	DPC					1164:1166	DPC	1164:1166	DPC	1164:1166	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	9	40	dep	membranes	1409:1417	arg1	the					1376:1378	the	1376:1378	the	1376:1378	Of-Pis1 interacts with both Escherichia coli and mammalian cell membranes, but only in the presence of Escherichia coli membranes it populates the helical conformation.					
36328080	9	40	dep	membranes	1409:1417	arg1	presence					1380:1387	presence	1380:1387	presence	1380:1387	Of-Pis1 interacts with both Escherichia coli and mammalian cell membranes, but only in the presence of Escherichia coli membranes it populates the helical conformation.					
36328080	12	41	theme	useful	1843:1848	arg1	insights					1834:1841	insights	1834:1841	insights useful to design more potent and selective AMPs	1834:1889	The conformational information is discussed in terms of Of-Pis1 amino acid sequence and composition to provide insights useful to design more potent and selective AMPs.					
36328080	1	42	attach	isolated	193:200	arg2	AMP					178:180	AMP	178:180	AMP	178:180	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	1	42	attach	isolated	193:200	arg2	peptide					169:175	a potent piscidin antimicrobial peptide	137:175	a potent piscidin antimicrobial peptide (AMP)	137:181	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	1	42	attach	isolated	193:200	arg2	Of-Pis1					126:132	Of-Pis1	126:132	Of-Pis1	126:132	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	1	42	attach	isolated	193:200	arg1	bream					212:216	rock bream	207:216	rock bream	207:216	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	2	43	theme	broad	320:324	arg1	activity					340:347	high and broad antimicrobial activity	311:347	high and broad antimicrobial activity	311:347	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	12	44	theme	sequence	1798:1805	arg1	terms					1770:1774	terms	1770:1774	terms of Of-Pis1 amino acid sequence and composition	1770:1821	The conformational information is discussed in terms of Of-Pis1 amino acid sequence and composition to provide insights useful to design more potent and selective AMPs.					
36328080	2	45	theme	hemolytic	368:376	arg1	toxicity					378:385	no significant hemolytic toxicity	353:385	no significant hemolytic toxicity	353:385	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	12	46	theme	composition	1811:1821	arg1	terms					1770:1774	terms	1770:1774	terms of Of-Pis1 amino acid sequence and composition	1770:1821	The conformational information is discussed in terms of Of-Pis1 amino acid sequence and composition to provide insights useful to design more potent and selective AMPs.					
36328080	10	47	theme	specific	1527:1534	arg1	interaction					1536:1546	a tighter and more specific interaction	1508:1546	a tighter and more specific interaction with bacterial than with eukaryotic membranes	1508:1592	Furthermore, ligand-based NMR experiments support a tighter and more specific interaction with bacterial than with eukaryotic membranes.					
36328080	3	48	theme	potential	504:512	arg1	selectivity					514:524	its potential selectivity	500:524	its potential selectivity	500:524	To better understand the mechanism of action of Of-Pis1 and its potential selectivity, using NMR and CD spectroscopies, we studied the interaction with eukaryotic and procaryotic membranes and membrane models.					
36328080	10	49	theme	eukaryotic	1573:1582	arg1	membranes					1584:1592	eukaryotic membranes	1573:1592	eukaryotic membranes	1573:1592	Furthermore, ligand-based NMR experiments support a tighter and more specific interaction with bacterial than with eukaryotic membranes.					
36328080	11	50	theme	selective	1632:1640	arg1	interaction					1642:1652	the selective interaction	1628:1652	the selective interaction of this broadly active AMP with bacterial over eukaryotic membranes	1628:1720	Overall, these data clearly show the selective interaction of this broadly active AMP with bacterial over eukaryotic membranes.					
36328080	7	51	theme	solvent	1090:1096	arg1	exchange					1098:1105	Slower solvent exchange	1083:1105	Slower solvent exchange	1083:1105	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	2	52	theme	24-amino	280:287	arg1	peptide					294:300	This rich in histidines and glycines 24-amino acid peptide	243:300	This rich in histidines and glycines 24-amino acid peptide	243:300	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	9	53	theme	Escherichia	1392:1402	arg1	membranes					1409:1417	Escherichia coli membranes	1392:1417	Escherichia coli membranes	1392:1417	Of-Pis1 interacts with both Escherichia coli and mammalian cell membranes, but only in the presence of Escherichia coli membranes it populates the helical conformation.					
36328080	12	54	theme	amino	1787:1791	arg1	sequence					1798:1805	Of-Pis1 amino acid sequence	1779:1805	Of-Pis1 amino acid sequence	1779:1805	The conformational information is discussed in terms of Of-Pis1 amino acid sequence and composition to provide insights useful to design more potent and selective AMPs.					
36328080	11	55	theme	AMP	1677:1679	arg1	interaction					1642:1652	the selective interaction	1628:1652	the selective interaction of this broadly active AMP with bacterial over eukaryotic membranes	1628:1720	Overall, these data clearly show the selective interaction of this broadly active AMP with bacterial over eukaryotic membranes.					
36328080	3	56	theme	NMR	533:535	arg1	spectroscopies					544:557	NMR and CD spectroscopies	533:557	spectroscopies	544:557	To better understand the mechanism of action of Of-Pis1 and its potential selectivity, using NMR and CD spectroscopies, we studied the interaction with eukaryotic and procaryotic membranes and membrane models.					
36328080	4	57	theme	eukaryotic	827:836	arg1	surrogate					847:855	eukaryotic membrane surrogate	827:855	eukaryotic membrane surrogate	827:855	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	57	theme	eukaryotic	827:836	arg1	phosphocholine					794:807	zwitterionic dodecyl phosphocholine	773:807	zwitterionic dodecyl phosphocholine (DPC)	773:813	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	58	theme	dodecyl	665:671	arg1	sulfate					673:679	Anionic sodium dodecyl sulfate	650:679	Anionic sodium dodecyl sulfate (SDS)	650:685	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	58	theme	dodecyl	665:671	arg1	SDS					682:684	SDS	682:684	SDS	682:684	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	2	59	theme	high	311:314	arg1	activity					340:347	high and broad antimicrobial activity	311:347	high and broad antimicrobial activity	311:347	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	5	60	theme	aqueous	864:870	arg1	environment					872:882	an aqueous environment	861:882	an aqueous environment	861:882	In an aqueous environment, Of-Pis1 adopts a flexible random coil conformation.					
36328080	9	61	theme	helical	1436:1442	arg1	conformation					1444:1455	the helical conformation	1432:1455	the helical conformation	1432:1455	Of-Pis1 interacts with both Escherichia coli and mammalian cell membranes, but only in the presence of Escherichia coli membranes it populates the helical conformation.					
36328080	3	62	theme	eukaryotic	592:601	arg1	membranes					619:627	eukaryotic and procaryotic membranes	592:627	eukaryotic and procaryotic membranes	592:627	To better understand the mechanism of action of Of-Pis1 and its potential selectivity, using NMR and CD spectroscopies, we studied the interaction with eukaryotic and procaryotic membranes and membrane models.					
36328080	1	63	theme	rock	207:210	arg1	bream					212:216	rock bream	207:216	rock bream	207:216	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	4	64	theme	Anionic	650:656	arg1	sulfate					673:679	Anionic sodium dodecyl sulfate	650:679	Anionic sodium dodecyl sulfate (SDS)	650:685	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	64	theme	Anionic	650:656	arg1	SDS					682:684	SDS	682:684	SDS	682:684	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	5	65	theme	flexible	902:909	arg1	conformation					923:934	a flexible random coil conformation	900:934	a flexible random coil conformation	900:934	In an aqueous environment, Of-Pis1 adopts a flexible random coil conformation.					
36328080	9	66	dep	Escherichia	1392:1402	arg1	coli					1404:1407	coli	1404:1407	coli	1404:1407	Of-Pis1 interacts with both Escherichia coli and mammalian cell membranes, but only in the presence of Escherichia coli membranes it populates the helical conformation.					
36328080	5	67	theme	coil	918:921	arg1	conformation					923:934	a flexible random coil conformation	900:934	a flexible random coil conformation	900:934	In an aqueous environment, Of-Pis1 adopts a flexible random coil conformation.					
36328080	2	68	from	histidines	256:265	arg1	rich					248:251	rich	248:251	rich	248:251	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	8	69	theme	LPS	1275:1277	arg1	micelles					1279:1286	perturbs LPS micelles	1266:1286	perturbs LPS micelles	1266:1286	Of-Pis1 also binds tightly and structurally perturbs LPS micelles.					
36328080	0	70	theme	piscidin	71:78	arg1	Of-Pis1					102:108	the histidine-rich piscidin antimicrobial peptide Of-Pis1	52:108	the histidine-rich piscidin antimicrobial peptide Of-Pis1	52:108	Structural insights on the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes.					
36328080	3	71	theme	procaryotic	607:617	arg1	membranes					619:627	eukaryotic and procaryotic membranes	592:627	eukaryotic and procaryotic membranes	592:627	To better understand the mechanism of action of Of-Pis1 and its potential selectivity, using NMR and CD spectroscopies, we studied the interaction with eukaryotic and procaryotic membranes and membrane models.					
36328080	1	72	theme	piscidin	146:153	arg1	AMP					178:180	AMP	178:180	AMP	178:180	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	1	72	theme	piscidin	146:153	arg1	peptide					169:175	a potent piscidin antimicrobial peptide	137:175	a potent piscidin antimicrobial peptide (AMP)	137:181	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	1	72	theme	piscidin	146:153	arg1	Of-Pis1					126:132	Of-Pis1	126:132	Of-Pis1	126:132	Of-Pis1 is a potent piscidin antimicrobial peptide (AMP), recently isolated from rock bream (Oplegnathus fasciatus).					
36328080	2	73	from	glycines	271:278	arg1	rich					248:251	rich	248:251	rich	248:251	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	7	74	from	pKas	1118:1121	arg1	SDS					1152:1154	SDS	1152:1154	SDS	1152:1154	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	7	74	from	pKas	1118:1121	arg1	DPC					1164:1166	DPC	1164:1166	DPC	1164:1166	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	0	75	theme	peptide	94:100	arg1	Of-Pis1					102:108	the histidine-rich piscidin antimicrobial peptide Of-Pis1	52:108	the histidine-rich piscidin antimicrobial peptide Of-Pis1	52:108	Structural insights on the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes.					
36328080	6	76	theme	Of-Pis1	986:992	arg1	region					976:981	the N-terminal region	961:981	the N-terminal region of Of-Pis1	961:992	In DPC and SDS instead, the N-terminal region of Of-Pis1 forms an amphipathic α-helix with the non-polar face in close contact with the micelles.					
36328080	0	77	from	insights	11:18	arg1	interaction					37:47	the selective interaction	23:47	the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes	23:123	Structural insights on the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes.					
36328080	4	78	theme	lipopolysaccharide	691:708	arg1	micelles					716:723	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles	650:723	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles	650:723	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	3	79	with	interaction	575:585	arg1	membranes					619:627	eukaryotic and procaryotic membranes	592:627	eukaryotic and procaryotic membranes	592:627	To better understand the mechanism of action of Of-Pis1 and its potential selectivity, using NMR and CD spectroscopies, we studied the interaction with eukaryotic and procaryotic membranes and membrane models.					
36328080	3	79	with	interaction	575:585	arg1	models					642:647	membrane models	633:647	membrane models	633:647	To better understand the mechanism of action of Of-Pis1 and its potential selectivity, using NMR and CD spectroscopies, we studied the interaction with eukaryotic and procaryotic membranes and membrane models.					
36328080	7	80	from	residues	1140:1147	arg1	SDS					1152:1154	SDS	1152:1154	SDS	1152:1154	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	7	81	from	SDS	1152:1154	arg1	exchange					1098:1105	Slower solvent exchange	1083:1105	Slower solvent exchange	1083:1105	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	7	81	from	SDS	1152:1154	arg1	pKas					1118:1121	higher pKas	1111:1121	higher pKas of the histidine residues in SDS than in DPC	1111:1166	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	2	82	theme	human	395:399	arg1	erythrocytes					401:412	human erythrocytes	395:412	human erythrocytes	395:412	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	6	83	theme	close	1050:1054	arg1	contact					1056:1062	close contact	1050:1062	close contact with the micelles	1050:1080	In DPC and SDS instead, the N-terminal region of Of-Pis1 forms an amphipathic α-helix with the non-polar face in close contact with the micelles.					
36328080	7	84	theme	residues	1140:1147	arg1	exchange					1098:1105	Slower solvent exchange	1083:1105	Slower solvent exchange	1083:1105	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	7	84	theme	residues	1140:1147	arg1	pKas					1118:1121	higher pKas	1111:1121	higher pKas of the histidine residues in SDS than in DPC	1111:1166	Slower solvent exchange and higher pKas of the histidine residues in SDS than in DPC suggest that Of-Pis1 interacts more tightly with SDS.					
36328080	12	85	theme	selective	1876:1884	arg1	AMPs					1886:1889	more potent and selective AMPs	1860:1889	more potent and selective AMPs	1860:1889	The conformational information is discussed in terms of Of-Pis1 amino acid sequence and composition to provide insights useful to design more potent and selective AMPs.					
36328080	10	86	theme	NMR	1484:1486	arg1	experiments					1488:1498	ligand-based NMR experiments	1471:1498	ligand-based NMR experiments	1471:1498	Furthermore, ligand-based NMR experiments support a tighter and more specific interaction with bacterial than with eukaryotic membranes.					
36328080	3	87	theme	action	478:483	arg1	mechanism					465:473	the mechanism	461:473	the mechanism of action of Of-Pis1 and its potential selectivity	461:524	To better understand the mechanism of action of Of-Pis1 and its potential selectivity, using NMR and CD spectroscopies, we studied the interaction with eukaryotic and procaryotic membranes and membrane models.					
36328080	2	88	from	rich	248:251	arg1	glycines					271:278	glycines	271:278	glycines	271:278	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	2	88	from	rich	248:251	arg1	histidines					256:265	histidines	256:265	histidines	256:265	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	9	89	theme	cell	1348:1351	arg1	membranes					1353:1361	mammalian cell membranes	1338:1361	mammalian cell membranes	1338:1361	Of-Pis1 interacts with both Escherichia coli and mammalian cell membranes, but only in the presence of Escherichia coli membranes it populates the helical conformation.					
36328080	6	90	with	contact	1056:1062	arg1	micelles					1073:1080	the micelles	1069:1080	the micelles	1069:1080	In DPC and SDS instead, the N-terminal region of Of-Pis1 forms an amphipathic α-helix with the non-polar face in close contact with the micelles.					
36328080	10	91	theme	tighter	1510:1516	arg1	interaction					1536:1546	a tighter and more specific interaction	1508:1546	a tighter and more specific interaction with bacterial than with eukaryotic membranes	1508:1592	Furthermore, ligand-based NMR experiments support a tighter and more specific interaction with bacterial than with eukaryotic membranes.					
36328080	2	92	theme	antimicrobial	326:338	arg1	activity					340:347	high and broad antimicrobial activity	311:347	high and broad antimicrobial activity	311:347	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36328080	12	93	theme	potent	1865:1870	arg1	AMPs					1886:1889	more potent and selective AMPs	1860:1889	more potent and selective AMPs	1860:1889	The conformational information is discussed in terms of Of-Pis1 amino acid sequence and composition to provide insights useful to design more potent and selective AMPs.					
36328080	4	94	theme	dodecyl	786:792	arg1	surrogate					847:855	eukaryotic membrane surrogate	827:855	eukaryotic membrane surrogate	827:855	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	94	theme	dodecyl	786:792	arg1	DPC					810:812	DPC	810:812	DPC	810:812	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	4	94	theme	dodecyl	786:792	arg1	phosphocholine					794:807	zwitterionic dodecyl phosphocholine	773:807	zwitterionic dodecyl phosphocholine (DPC)	773:813	Anionic sodium dodecyl sulfate (SDS) and lipopolysaccharide (LPS) micelles were used to mimic procaryotic membranes, while zwitterionic dodecyl phosphocholine (DPC) was used as eukaryotic membrane surrogate.					
36328080	3	95	theme	Of-Pis1	488:494	arg1	action					478:483	action	478:483	action of Of-Pis1 and its potential selectivity	478:524	To better understand the mechanism of action of Of-Pis1 and its potential selectivity, using NMR and CD spectroscopies, we studied the interaction with eukaryotic and procaryotic membranes and membrane models.					
36328080	12	96	theme	acid	1793:1796	arg1	sequence					1798:1805	Of-Pis1 amino acid sequence	1779:1805	Of-Pis1 amino acid sequence	1779:1805	The conformational information is discussed in terms of Of-Pis1 amino acid sequence and composition to provide insights useful to design more potent and selective AMPs.					
36328080	0	97	with	interaction	37:47	arg1	membranes					115:123	membranes	115:123	membranes	115:123	Structural insights on the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes.					
36328080	2	98	theme	significant	356:366	arg1	toxicity					378:385	no significant hemolytic toxicity	353:385	no significant hemolytic toxicity	353:385	This rich in histidines and glycines 24-amino acid peptide displays high and broad antimicrobial activity and no significant hemolytic toxicity against human erythrocytes, suggesting low toxicity.					
36195631	0	0	theme	milk	72:75	arg1	composition					44:54	the composition	40:54	the composition of human breast milk	40:75	Cannabis use during lactation may alter the composition of human breast milk.					
36195631	6	1	theme	women	874:878	arg1	milk					866:869	the milk	862:869	the milk of women who reported cannabis use during lactation (n = 13, median: 22 ng/mL)	862:948	RESULTS THC was detected in the milk of women who reported cannabis use during lactation (n = 13, median: 22 ng/mL).					
36195631	8	2	theme	SIgA	1126:1129	arg1	levels					1131:1136	SIgA levels	1126:1136	SIgA levels	1126:1136	Relative to non-users (n = 17), lactose levels were higher and SIgA levels were significantly lower in the milk of subjects who used cannabis during lactation (n = 14).					
36195631	3	3	from	changes	486:492	arg1	levels					501:506	the levels	497:506	the levels of macronutrients and bioactive factors in breast milk	497:561	The objective of this study was to investigate the effect of maternal cannabis use on changes in the levels of macronutrients and bioactive factors in breast milk.					
36195631	9	4	theme	cannabinoids	1260:1271	arg1	presence					1248:1255	The presence	1244:1255	The presence of cannabinoids	1244:1271	CONCLUSIONS The presence of cannabinoids, along with altered lactose and SIgA levels in the milk of cannabis users, may have implications for infant health.					
36195631	0	5	theme	breast	65:70	arg1	milk					72:75	human breast milk	59:75	human breast milk	59:75	Cannabis use during lactation may alter the composition of human breast milk.					
36195631	6	6	theme	RESULTS	834:840	arg1	THC					842:844	RESULTS THC	834:844	RESULTS THC	834:844	RESULTS THC was detected in the milk of women who reported cannabis use during lactation (n = 13, median: 22 ng/mL).					
36195631	12	7	theme	significant	1841:1851	arg1	implications					1853:1864	significant implications	1841:1864	significant implications	1841:1864	Change in levels of lactose and SIgA in the milk of cannabis users may have significant implications on infant health, which must be investigated in the future to better inform mothers.					
36195631	3	8	theme	maternal	461:468	arg1	use					479:481	maternal cannabis use	461:481	maternal cannabis use	461:481	The objective of this study was to investigate the effect of maternal cannabis use on changes in the levels of macronutrients and bioactive factors in breast milk.					
36195631	2	9	attach	presence	312:319	arg2	cannabinoids					324:335	cannabinoids	324:335	cannabinoids	324:335	While exclusive breastfeeding has been recommended for the first 6 months of an infant's life, the presence of cannabinoids in the milk of cannabis users complicates this recommendation.					
36195631	2	9	attach	presence	312:319	arg1	milk					344:347	the milk	340:347	the milk of cannabis users	340:365	While exclusive breastfeeding has been recommended for the first 6 months of an infant's life, the presence of cannabinoids in the milk of cannabis users complicates this recommendation.					
36195631	6	10	located	detected	850:857	arg1	milk					866:869	the milk	862:869	the milk of women who reported cannabis use during lactation (n = 13, median: 22 ng/mL)	862:948	RESULTS THC was detected in the milk of women who reported cannabis use during lactation (n = 13, median: 22 ng/mL).					
36195631	6	10	located	detected	850:857	arg2	THC					842:844	RESULTS THC	834:844	RESULTS THC	834:844	RESULTS THC was detected in the milk of women who reported cannabis use during lactation (n = 13, median: 22 ng/mL).					
36195631	2	11	theme	first	272:276	arg1	months					280:285	the first 6 months	268:285	the first 6 months of an infant's life	268:305	While exclusive breastfeeding has been recommended for the first 6 months of an infant's life, the presence of cannabinoids in the milk of cannabis users complicates this recommendation.					
36195631	12	12	theme	lactose	1785:1791	arg1	levels					1775:1780	levels	1775:1780	levels of lactose and SIgA	1775:1800	Change in levels of lactose and SIgA in the milk of cannabis users may have significant implications on infant health, which must be investigated in the future to better inform mothers.					
36195631	10	13	theme	potential	1539:1547	arg1	exposure					1549:1556	potential exposure	1539:1556	potential exposure among infants born to mothers who consume cannabis	1539:1607	IMPACT Metabolites of cannabis are found in breast milk and can accumulate in higher concentrations with ongoing consumption, which is concerning for potential exposure among infants born to mothers who consume cannabis.					
36195631	4	14	theme	used	682:685	arg1	cannabis					687:694	used cannabis	682:694	used cannabis	682:694	METHODS Milk was collected from women who were 6-8 weeks postpartum and were either using cannabis post-delivery, had used cannabis during pregnancy, or were non-users.					
36195631	6	15	dep	n = 13	924:929	arg1	22 ng/mL					940:947	22 ng/mL	940:947	22 ng/mL	940:947	RESULTS THC was detected in the milk of women who reported cannabis use during lactation (n = 13, median: 22 ng/mL).					
36195631	11	16	theme	breast	1699:1704	arg1	milk					1706:1709	the breast milk	1695:1709	the breast milk of cannabis users	1695:1727	This work reports that lactose levels are increased and SIgA levels are decreased in the breast milk of cannabis users, relative to the milk of non-users.					
36195631	6	17	theme	cannabis	893:900	arg1	use					902:904	cannabis use	893:904	cannabis use	893:904	RESULTS THC was detected in the milk of women who reported cannabis use during lactation (n = 13, median: 22 ng/mL).					
36195631	12	18	theme	SIgA	1797:1800	arg1	levels					1775:1780	levels	1775:1780	levels of lactose and SIgA	1775:1800	Change in levels of lactose and SIgA in the milk of cannabis users may have significant implications on infant health, which must be investigated in the future to better inform mothers.					
36195631	7	19	located	detected	1003:1010	arg2	CBD					980:982	CBD	980:982	CBD	980:982	Carboxy-THC, 11-hydroxy-THC, CBD, and CBN were also detected in the milk of women who used cannabis postpartum.					
36195631	7	19	located	detected	1003:1010	arg2	11-hydroxy-THC					964:977	11-hydroxy-THC	964:977	11-hydroxy-THC	964:977	Carboxy-THC, 11-hydroxy-THC, CBD, and CBN were also detected in the milk of women who used cannabis postpartum.					
36195631	7	19	located	detected	1003:1010	arg2	CBN					989:991	CBN	989:991	CBN	989:991	Carboxy-THC, 11-hydroxy-THC, CBD, and CBN were also detected in the milk of women who used cannabis postpartum.					
36195631	7	19	located	detected	1003:1010	arg1	milk					1019:1022	the milk	1015:1022	the milk of women who used cannabis postpartum	1015:1060	Carboxy-THC, 11-hydroxy-THC, CBD, and CBN were also detected in the milk of women who used cannabis postpartum.					
36195631	7	19	located	detected	1003:1010	arg2	Carboxy-THC					951:961	Carboxy-THC	951:961	Carboxy-THC	951:961	Carboxy-THC, 11-hydroxy-THC, CBD, and CBN were also detected in the milk of women who used cannabis postpartum.					
36195631	11	20	theme	users	1723:1727	arg1	milk					1706:1709	the breast milk	1695:1709	the breast milk of cannabis users	1695:1727	This work reports that lactose levels are increased and SIgA levels are decreased in the breast milk of cannabis users, relative to the milk of non-users.					
36195631	1	21	theme	morning	143:149	arg1	sickness					151:158	morning sickness	143:158	morning sickness	143:158	BACKGROUND Cannabis is often used by women to manage symptoms of morning sickness during pregnancy, and postpartum stress and anxiety.					
36195631	2	22	from	presence	312:319	arg1	milk					344:347	the milk	340:347	the milk of cannabis users	340:365	While exclusive breastfeeding has been recommended for the first 6 months of an infant's life, the presence of cannabinoids in the milk of cannabis users complicates this recommendation.					
36195631	4	23	dep	postpartum	621:630	arg1	had					678:680	had	678:680	had used cannabis during pregnancy	678:711	METHODS Milk was collected from women who were 6-8 weeks postpartum and were either using cannabis post-delivery, had used cannabis during pregnancy, or were non-users.					
36195631	1	24	theme	sickness	151:158	arg1	symptoms					131:138	symptoms	131:138	symptoms of morning sickness	131:158	BACKGROUND Cannabis is often used by women to manage symptoms of morning sickness during pregnancy, and postpartum stress and anxiety.					
36195631	5	25	theme	cannabinoids	743:754	arg1	Levels					733:738	Levels	733:738	Levels of cannabinoids, macronutrients, lactose, and SIgA	733:789	Levels of cannabinoids, macronutrients, lactose, and SIgA were assessed in the milk of all subjects.					
36195631	11	26	theme	lactose	1633:1639	arg1	levels					1641:1646	lactose levels	1633:1646	lactose levels	1633:1646	This work reports that lactose levels are increased and SIgA levels are decreased in the breast milk of cannabis users, relative to the milk of non-users.					
36195631	10	27	theme	breast	1433:1438	arg1	milk					1440:1443	breast milk	1433:1443	breast milk	1433:1443	IMPACT Metabolites of cannabis are found in breast milk and can accumulate in higher concentrations with ongoing consumption, which is concerning for potential exposure among infants born to mothers who consume cannabis.					
36195631	10	28	theme	IMPACT	1389:1394	arg1	Metabolites					1396:1406	IMPACT Metabolites	1389:1406	IMPACT Metabolites of cannabis	1389:1418	IMPACT Metabolites of cannabis are found in breast milk and can accumulate in higher concentrations with ongoing consumption, which is concerning for potential exposure among infants born to mothers who consume cannabis.					
36195631	3	29	theme	macronutrients	511:524	arg1	levels					501:506	the levels	497:506	the levels of macronutrients and bioactive factors in breast milk	497:561	The objective of this study was to investigate the effect of maternal cannabis use on changes in the levels of macronutrients and bioactive factors in breast milk.					
36195631	7	30	theme	cannabis	1042:1049	arg1	postpartum					1051:1060	cannabis postpartum	1042:1060	cannabis postpartum	1042:1060	Carboxy-THC, 11-hydroxy-THC, CBD, and CBN were also detected in the milk of women who used cannabis postpartum.					
36195631	8	31	used	used	1191:1194	arg2	subjects					1178:1185	subjects	1178:1185	subjects who used cannabis during lactation (n = 14)	1178:1229	Relative to non-users (n = 17), lactose levels were higher and SIgA levels were significantly lower in the milk of subjects who used cannabis during lactation (n = 14).					
36195631	0	32	theme	Cannabis	0:7	arg1	use					9:11	Cannabis use	0:11	Cannabis use during lactation	0:28	Cannabis use during lactation may alter the composition of human breast milk.					
36195631	1	33	theme	BACKGROUND	78:87	arg1	Cannabis					89:96	BACKGROUND Cannabis	78:96	BACKGROUND Cannabis	78:96	BACKGROUND Cannabis is often used by women to manage symptoms of morning sickness during pregnancy, and postpartum stress and anxiety.					
36195631	9	34	theme	SIgA	1305:1308	arg1	levels					1310:1315	altered lactose and SIgA levels	1285:1315	altered lactose and SIgA levels in the milk of cannabis users	1285:1345	CONCLUSIONS The presence of cannabinoids, along with altered lactose and SIgA levels in the milk of cannabis users, may have implications for infant health.					
36195631	5	35	theme	macronutrients	757:770	arg1	Levels					733:738	Levels	733:738	Levels of cannabinoids, macronutrients, lactose, and SIgA	733:789	Levels of cannabinoids, macronutrients, lactose, and SIgA were assessed in the milk of all subjects.					
36195631	7	36	used	used	1037:1040	arg2	women					1027:1031	women	1027:1031	women who used cannabis postpartum	1027:1060	Carboxy-THC, 11-hydroxy-THC, CBD, and CBN were also detected in the milk of women who used cannabis postpartum.					
36195631	3	37	from	effect	451:456	arg1	changes					486:492	changes	486:492	changes in the levels of macronutrients and bioactive factors in breast milk	486:561	The objective of this study was to investigate the effect of maternal cannabis use on changes in the levels of macronutrients and bioactive factors in breast milk.					
36195631	1	38	used	used	107:110	arg2	Cannabis					89:96	BACKGROUND Cannabis	78:96	BACKGROUND Cannabis	78:96	BACKGROUND Cannabis is often used by women to manage symptoms of morning sickness during pregnancy, and postpartum stress and anxiety.					
36195631	3	39	theme	study	422:426	arg1	objective					404:412	The objective	400:412	The objective of this study	400:426	The objective of this study was to investigate the effect of maternal cannabis use on changes in the levels of macronutrients and bioactive factors in breast milk.					
36195631	12	40	contain	have	1836:1839	arg1	Change					1765:1770	Change	1765:1770	Change in levels of lactose and SIgA in the milk of cannabis users	1765:1830	Change in levels of lactose and SIgA in the milk of cannabis users may have significant implications on infant health, which must be investigated in the future to better inform mothers.					
36195631	12	40	contain	have	1836:1839	arg2	implications					1853:1864	significant implications	1841:1864	significant implications	1841:1864	Change in levels of lactose and SIgA in the milk of cannabis users may have significant implications on infant health, which must be investigated in the future to better inform mothers.					
36195631	4	41	contain	had	678:680	arg1	women					596:600	women	596:600	women who were 6-8 weeks postpartum and were either using cannabis post-delivery, had used cannabis during pregnancy, or were non-users	596:730	METHODS Milk was collected from women who were 6-8 weeks postpartum and were either using cannabis post-delivery, had used cannabis during pregnancy, or were non-users.					
36195631	4	41	contain	had	678:680	arg2	cannabis					687:694	used cannabis	682:694	used cannabis	682:694	METHODS Milk was collected from women who were 6-8 weeks postpartum and were either using cannabis post-delivery, had used cannabis during pregnancy, or were non-users.					
36195631	12	42	theme	users	1826:1830	arg1	milk					1809:1812	the milk	1805:1812	the milk of cannabis users	1805:1830	Change in levels of lactose and SIgA in the milk of cannabis users may have significant implications on infant health, which must be investigated in the future to better inform mothers.					
36195631	6	43	dep	lactation	913:921	arg1	n = 13					924:929	n = 13	924:929	n = 13	924:929	RESULTS THC was detected in the milk of women who reported cannabis use during lactation (n = 13, median: 22 ng/mL).					
36195631	6	43	dep	lactation	913:921	arg1	median					932:937	median	932:937	median	932:937	RESULTS THC was detected in the milk of women who reported cannabis use during lactation (n = 13, median: 22 ng/mL).					
36195631	5	44	theme	subjects	824:831	arg1	milk					812:815	the milk	808:815	the milk of all subjects	808:831	Levels of cannabinoids, macronutrients, lactose, and SIgA were assessed in the milk of all subjects.					
36195631	2	45	theme	cannabinoids	324:335	arg1	presence					312:319	the presence	308:319	the presence of cannabinoids in the milk of cannabis users	308:365	While exclusive breastfeeding has been recommended for the first 6 months of an infant's life, the presence of cannabinoids in the milk of cannabis users complicates this recommendation.					
36195631	8	46	theme	subjects	1178:1185	arg1	milk					1170:1173	the milk	1166:1173	the milk of subjects who used cannabis during lactation (n = 14)	1166:1229	Relative to non-users (n = 17), lactose levels were higher and SIgA levels were significantly lower in the milk of subjects who used cannabis during lactation (n = 14).					
36195631	9	47	theme	cannabis	1332:1339	arg1	users					1341:1345	cannabis users	1332:1345	cannabis users	1332:1345	CONCLUSIONS The presence of cannabinoids, along with altered lactose and SIgA levels in the milk of cannabis users, may have implications for infant health.					
36195631	10	48	theme	higher	1467:1472	arg1	concentrations					1474:1487	higher concentrations	1467:1487	higher concentrations	1467:1487	IMPACT Metabolites of cannabis are found in breast milk and can accumulate in higher concentrations with ongoing consumption, which is concerning for potential exposure among infants born to mothers who consume cannabis.					
36195631	2	49	theme	users	361:365	arg1	milk					344:347	the milk	340:347	the milk of cannabis users	340:365	While exclusive breastfeeding has been recommended for the first 6 months of an infant's life, the presence of cannabinoids in the milk of cannabis users complicates this recommendation.					
36195631	7	50	theme	women	1027:1031	arg1	milk					1019:1022	the milk	1015:1022	the milk of women who used cannabis postpartum	1015:1060	Carboxy-THC, 11-hydroxy-THC, CBD, and CBN were also detected in the milk of women who used cannabis postpartum.					
36195631	11	51	theme	SIgA	1666:1669	arg1	levels					1671:1676	SIgA levels	1666:1676	SIgA levels	1666:1676	This work reports that lactose levels are increased and SIgA levels are decreased in the breast milk of cannabis users, relative to the milk of non-users.					
36195631	10	52	theme	cannabis	1411:1418	arg1	Metabolites					1396:1406	IMPACT Metabolites	1389:1406	IMPACT Metabolites of cannabis	1389:1418	IMPACT Metabolites of cannabis are found in breast milk and can accumulate in higher concentrations with ongoing consumption, which is concerning for potential exposure among infants born to mothers who consume cannabis.					
36195631	9	53	dep	CONCLUSIONS	1232:1242	arg1	have					1352:1355	have	1352:1355	may have implications for infant health	1348:1386	CONCLUSIONS The presence of cannabinoids, along with altered lactose and SIgA levels in the milk of cannabis users, may have implications for infant health.					
36195631	3	54	theme	breast	551:556	arg1	milk					558:561	breast milk	551:561	breast milk	551:561	The objective of this study was to investigate the effect of maternal cannabis use on changes in the levels of macronutrients and bioactive factors in breast milk.					
36195631	12	55	theme	infant	1869:1874	arg1	health					1876:1881	infant health	1869:1881	infant health	1869:1881	Change in levels of lactose and SIgA in the milk of cannabis users may have significant implications on infant health, which must be investigated in the future to better inform mothers.					
36195631	2	56	theme	cannabis	352:359	arg1	users					361:365	cannabis users	352:365	cannabis users	352:365	While exclusive breastfeeding has been recommended for the first 6 months of an infant's life, the presence of cannabinoids in the milk of cannabis users complicates this recommendation.					
36195631	9	57	theme	lactose	1293:1299	arg1	levels					1310:1315	altered lactose and SIgA levels	1285:1315	altered lactose and SIgA levels in the milk of cannabis users	1285:1345	CONCLUSIONS The presence of cannabinoids, along with altered lactose and SIgA levels in the milk of cannabis users, may have implications for infant health.					
36195631	3	58	theme	bioactive	530:538	arg1	factors					540:546	bioactive factors	530:546	bioactive factors	530:546	The objective of this study was to investigate the effect of maternal cannabis use on changes in the levels of macronutrients and bioactive factors in breast milk.					
36195631	9	59	theme	altered	1285:1291	arg1	levels					1310:1315	altered lactose and SIgA levels	1285:1315	altered lactose and SIgA levels in the milk of cannabis users	1285:1345	CONCLUSIONS The presence of cannabinoids, along with altered lactose and SIgA levels in the milk of cannabis users, may have implications for infant health.					
36195631	4	60	theme	cannabis	654:661	arg1	post-delivery					663:675	cannabis post-delivery	654:675	cannabis post-delivery	654:675	METHODS Milk was collected from women who were 6-8 weeks postpartum and were either using cannabis post-delivery, had used cannabis during pregnancy, or were non-users.					
36195631	3	61	theme	factors	540:546	arg1	levels					501:506	the levels	497:506	the levels of macronutrients and bioactive factors in breast milk	497:561	The objective of this study was to investigate the effect of maternal cannabis use on changes in the levels of macronutrients and bioactive factors in breast milk.					
36195631	9	62	from	levels	1310:1315	arg1	milk					1324:1327	the milk	1320:1327	the milk of cannabis users	1320:1345	CONCLUSIONS The presence of cannabinoids, along with altered lactose and SIgA levels in the milk of cannabis users, may have implications for infant health.					
36195631	2	63	theme	life	302:305	arg1	months					280:285	the first 6 months	268:285	the first 6 months of an infant's life	268:305	While exclusive breastfeeding has been recommended for the first 6 months of an infant's life, the presence of cannabinoids in the milk of cannabis users complicates this recommendation.					
36195631	3	64	from	levels	501:506	arg1	milk					558:561	breast milk	551:561	breast milk	551:561	The objective of this study was to investigate the effect of maternal cannabis use on changes in the levels of macronutrients and bioactive factors in breast milk.					
36195631	9	65	contain	have	1352:1355	arg1	presence					1248:1255	The presence	1244:1255	The presence of cannabinoids	1244:1271	CONCLUSIONS The presence of cannabinoids, along with altered lactose and SIgA levels in the milk of cannabis users, may have implications for infant health.					
36195631	9	65	contain	have	1352:1355	arg2	implications					1357:1368	implications	1357:1368	implications for infant health	1357:1386	CONCLUSIONS The presence of cannabinoids, along with altered lactose and SIgA levels in the milk of cannabis users, may have implications for infant health.					
36195631	1	66	dep	postpartum	182:191	arg1	stress					193:198	stress	193:198	stress	193:198	BACKGROUND Cannabis is often used by women to manage symptoms of morning sickness during pregnancy, and postpartum stress and anxiety.					
36195631	9	67	theme	users	1341:1345	arg1	milk					1324:1327	the milk	1320:1327	the milk of cannabis users	1320:1345	CONCLUSIONS The presence of cannabinoids, along with altered lactose and SIgA levels in the milk of cannabis users, may have implications for infant health.					
36195631	12	68	from	Change	1765:1770	arg1	milk					1809:1812	the milk	1805:1812	the milk of cannabis users	1805:1830	Change in levels of lactose and SIgA in the milk of cannabis users may have significant implications on infant health, which must be investigated in the future to better inform mothers.					
36195631	12	68	from	Change	1765:1770	arg1	levels					1775:1780	levels	1775:1780	levels of lactose and SIgA	1775:1800	Change in levels of lactose and SIgA in the milk of cannabis users may have significant implications on infant health, which must be investigated in the future to better inform mothers.					
36195631	9	69	theme	infant	1374:1379	arg1	health					1381:1386	infant health	1374:1386	infant health	1374:1386	CONCLUSIONS The presence of cannabinoids, along with altered lactose and SIgA levels in the milk of cannabis users, may have implications for infant health.					
36195631	11	70	theme	cannabis	1714:1721	arg1	users					1723:1727	cannabis users	1714:1727	cannabis users	1714:1727	This work reports that lactose levels are increased and SIgA levels are decreased in the breast milk of cannabis users, relative to the milk of non-users.					
36195631	0	71	theme	human	59:63	arg1	milk					72:75	human breast milk	59:75	human breast milk	59:75	Cannabis use during lactation may alter the composition of human breast milk.					
36195631	5	72	theme	SIgA	786:789	arg1	Levels					733:738	Levels	733:738	Levels of cannabinoids, macronutrients, lactose, and SIgA	733:789	Levels of cannabinoids, macronutrients, lactose, and SIgA were assessed in the milk of all subjects.					
36195631	10	73	located	found	1424:1428	arg1	milk					1440:1443	breast milk	1433:1443	breast milk	1433:1443	IMPACT Metabolites of cannabis are found in breast milk and can accumulate in higher concentrations with ongoing consumption, which is concerning for potential exposure among infants born to mothers who consume cannabis.					
36195631	10	73	located	found	1424:1428	arg2	Metabolites					1396:1406	IMPACT Metabolites	1389:1406	IMPACT Metabolites of cannabis	1389:1418	IMPACT Metabolites of cannabis are found in breast milk and can accumulate in higher concentrations with ongoing consumption, which is concerning for potential exposure among infants born to mothers who consume cannabis.					
36195631	5	74	theme	lactose	773:779	arg1	Levels					733:738	Levels	733:738	Levels of cannabinoids, macronutrients, lactose, and SIgA	733:789	Levels of cannabinoids, macronutrients, lactose, and SIgA were assessed in the milk of all subjects.					
36195631	3	75	theme	cannabis	470:477	arg1	use					479:481	maternal cannabis use	461:481	maternal cannabis use	461:481	The objective of this study was to investigate the effect of maternal cannabis use on changes in the levels of macronutrients and bioactive factors in breast milk.					
36195631	8	76	theme	lactose	1095:1101	arg1	levels					1103:1108	lactose levels	1095:1108	lactose levels	1095:1108	Relative to non-users (n = 17), lactose levels were higher and SIgA levels were significantly lower in the milk of subjects who used cannabis during lactation (n = 14).					
36195631	10	77	theme	ongoing	1494:1500	arg1	consumption					1502:1512	ongoing consumption	1494:1512	ongoing consumption	1494:1512	IMPACT Metabolites of cannabis are found in breast milk and can accumulate in higher concentrations with ongoing consumption, which is concerning for potential exposure among infants born to mothers who consume cannabis.					
36195631	12	78	theme	cannabis	1817:1824	arg1	users					1826:1830	cannabis users	1817:1830	cannabis users	1817:1830	Change in levels of lactose and SIgA in the milk of cannabis users may have significant implications on infant health, which must be investigated in the future to better inform mothers.					
36195631	11	79	theme	non-users	1754:1762	arg1	milk					1746:1749	the milk	1742:1749	the milk of non-users	1742:1762	This work reports that lactose levels are increased and SIgA levels are decreased in the breast milk of cannabis users, relative to the milk of non-users.					
36195631	2	80	theme	exclusive	219:227	arg1	breastfeeding					229:241	exclusive breastfeeding	219:241	exclusive breastfeeding	219:241	While exclusive breastfeeding has been recommended for the first 6 months of an infant's life, the presence of cannabinoids in the milk of cannabis users complicates this recommendation.					
36195631	3	81	theme	use	479:481	arg1	effect					451:456	the effect	447:456	the effect of maternal cannabis use on changes in the levels of macronutrients and bioactive factors in breast milk	447:561	The objective of this study was to investigate the effect of maternal cannabis use on changes in the levels of macronutrients and bioactive factors in breast milk.					
36195631	4	82	theme	METHODS	564:570	arg1	Milk					572:575	METHODS Milk	564:575	METHODS Milk	564:575	METHODS Milk was collected from women who were 6-8 weeks postpartum and were either using cannabis post-delivery, had used cannabis during pregnancy, or were non-users.					
36919574	3	0	theme	novel	474:478	arg1	system					617:622	a painless and patient-friendly long-term drug delivery system	561:622	a painless and patient-friendly long-term drug delivery system	561:622	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	0	theme	novel	474:478	arg1	TLM					553:555	TLM	553:555	TLM	553:555	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	0	theme	novel	474:478	arg1	microneedle					540:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle	472:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM)	472:556	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	6	1	theme	obese	1055:1059	arg1	mice					1061:1064	obese mice	1055:1064	obese mice	1055:1064	Additionally, it ameliorated diet-induced hepatic steatosis in obese mice.					
36919574	7	2	theme	release	1127:1133	arg1	system					1135:1140	a glucagon-like peptide-1 drug release system	1096:1140	a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection	1096:1252	This novel TLM could promote a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection.					
36919574	7	3	theme	higher	1193:1198	arg1	compliance					1208:1217	relatively higher patient compliance	1182:1217	relatively higher patient compliance	1182:1217	This novel TLM could promote a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection.					
36919574	2	4	theme	suppressant	344:354	arg1	injection					319:327	subcutaneous injection	306:327	subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region	306:419	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	7	5	theme	long-term	1146:1154	arg1	administration					1162:1175	long-term daily administration	1146:1175	long-term daily administration with relatively higher patient compliance compared to subcutaneous injection	1146:1252	This novel TLM could promote a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection.					
36919574	2	6	from	injection	319:327	arg1	region					414:419	the locally injected region	393:419	the locally injected region	393:419	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	4	7	theme	encapsulated	767:778	arg1	liraglutide					780:790	the encapsulated liraglutide	763:790	the encapsulated liraglutide	763:790	In contrast to previous anti-obesity microneedle approaches, this TLM is composed of three layers for complete skin insertion, protecting the encapsulated liraglutide from environmental stresses.					
36919574	5	8	theme	diet-induced	970:981	arg1	obesity					983:989	high-fat diet-induced obesity	961:989	high-fat diet-induced obesity	961:989	Daily topical application of the liraglutide-loaded TLM significantly reduced body weight and improved body composition in a mouse model of high-fat diet-induced obesity.					
36919574	6	9	theme	hepatic	1034:1040	arg1	steatosis					1042:1050	diet-induced hepatic steatosis	1021:1050	diet-induced hepatic steatosis in obese mice	1021:1064	Additionally, it ameliorated diet-induced hepatic steatosis in obese mice.					
36919574	1	10	theme	humans	258:263	arg1	longevity					245:253	longevity	245:253	longevity of humans	245:263	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36919574	1	10	theme	humans	258:263	arg1	quality					225:231	the quality	221:231	the quality of life	221:239	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36919574	0	11	theme	obesity	110:116	arg1	treatment					97:105	the treatment	93:105	the treatment of obesity	93:116	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.					
36919574	5	12	theme	mouse	946:950	arg1	model					952:956	a mouse model	944:956	a mouse model of high-fat diet-induced obesity	944:989	Daily topical application of the liraglutide-loaded TLM significantly reduced body weight and improved body composition in a mouse model of high-fat diet-induced obesity.					
36919574	6	13	from	steatosis	1042:1050	arg1	mice					1061:1064	obese mice	1055:1064	obese mice	1055:1064	Additionally, it ameliorated diet-induced hepatic steatosis in obese mice.					
36919574	5	14	theme	TLM	873:875	arg1	application					835:845	Daily topical application	821:845	Daily topical application of the liraglutide-loaded TLM	821:875	Daily topical application of the liraglutide-loaded TLM significantly reduced body weight and improved body composition in a mouse model of high-fat diet-induced obesity.					
36919574	3	15	theme	triple-layer	527:538	arg1	system					617:622	a painless and patient-friendly long-term drug delivery system	561:622	a painless and patient-friendly long-term drug delivery system	561:622	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	15	theme	triple-layer	527:538	arg1	TLM					553:555	TLM	553:555	TLM	553:555	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	15	theme	triple-layer	527:538	arg1	microneedle					540:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle	472:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM)	472:556	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	5	16	theme	body	899:902	arg1	weight					904:909	body weight	899:909	body weight	899:909	Daily topical application of the liraglutide-loaded TLM significantly reduced body weight and improved body composition in a mouse model of high-fat diet-induced obesity.					
36919574	5	17	theme	Daily	821:825	arg1	application					835:845	Daily topical application	821:845	Daily topical application of the liraglutide-loaded TLM	821:875	Daily topical application of the liraglutide-loaded TLM significantly reduced body weight and improved body composition in a mouse model of high-fat diet-induced obesity.					
36919574	7	18	theme	daily	1156:1160	arg1	administration					1162:1175	long-term daily administration	1146:1175	long-term daily administration with relatively higher patient compliance compared to subcutaneous injection	1146:1252	This novel TLM could promote a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection.					
36919574	1	19	theme	prevalent	166:174	arg1	worldwide					176:184	prevalent worldwide	166:184	prevalent worldwide	166:184	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36919574	1	19	theme	prevalent	166:174	arg1	disease					150:156	a chronic metabolic disease	130:156	a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans	130:263	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36919574	1	19	theme	prevalent	166:174	arg1	Obesity					119:125	Obesity	119:125	Obesity	119:125	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36919574	5	20	theme	topical	827:833	arg1	application					835:845	Daily topical application	821:845	Daily topical application of the liraglutide-loaded TLM	821:875	Daily topical application of the liraglutide-loaded TLM significantly reduced body weight and improved body composition in a mouse model of high-fat diet-induced obesity.					
36919574	3	21	theme	liraglutide-encapsulated	502:525	arg1	system					617:622	a painless and patient-friendly long-term drug delivery system	561:622	a painless and patient-friendly long-term drug delivery system	561:622	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	21	theme	liraglutide-encapsulated	502:525	arg1	TLM					553:555	TLM	553:555	TLM	553:555	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	21	theme	liraglutide-encapsulated	502:525	arg1	microneedle					540:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle	472:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM)	472:556	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	2	22	from	suppressant	344:354	arg1	region					414:419	the locally injected region	393:419	the locally injected region	393:419	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	2	23	theme	subcutaneous	306:317	arg1	injection					319:327	subcutaneous injection	306:327	subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region	306:419	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	3	24	theme	acid-based	491:500	arg1	system					617:622	a painless and patient-friendly long-term drug delivery system	561:622	a painless and patient-friendly long-term drug delivery system	561:622	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	24	theme	acid-based	491:500	arg1	TLM					553:555	TLM	553:555	TLM	553:555	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	24	theme	acid-based	491:500	arg1	microneedle					540:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle	472:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM)	472:556	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	2	25	theme	health	374:379	arg1	problems					381:388	health problems	374:388	health problems in the locally injected region	374:419	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	6	26	theme	diet-induced	1021:1032	arg1	steatosis					1042:1050	diet-induced hepatic steatosis	1021:1050	diet-induced hepatic steatosis in obese mice	1021:1064	Additionally, it ameliorated diet-induced hepatic steatosis in obese mice.					
36919574	3	27	theme	painless	563:570	arg1	system					617:622	a painless and patient-friendly long-term drug delivery system	561:622	a painless and patient-friendly long-term drug delivery system	561:622	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	27	theme	painless	563:570	arg1	microneedle					540:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle	472:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM)	472:556	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	5	28	theme	improved	915:922	arg1	composition					929:939	improved body composition	915:939	improved body composition	915:939	Daily topical application of the liraglutide-loaded TLM significantly reduced body weight and improved body composition in a mouse model of high-fat diet-induced obesity.					
36919574	0	29	theme	triple	40:45	arg1	TLMs					83:86	TLMs	83:86	TLMs	83:86	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.					
36919574	0	29	theme	triple	40:45	arg1	microneedles					69:80	liraglutide-encapsulated triple layer hyaluronic acid microneedles	15:80	liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs)	15:87	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.					
36919574	4	30	theme	skin	736:739	arg1	insertion					741:749	complete skin insertion	727:749	complete skin insertion	727:749	In contrast to previous anti-obesity microneedle approaches, this TLM is composed of three layers for complete skin insertion, protecting the encapsulated liraglutide from environmental stresses.					
36919574	7	31	theme	drug	1122:1125	arg1	system					1135:1140	a glucagon-like peptide-1 drug release system	1096:1140	a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection	1096:1252	This novel TLM could promote a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection.					
36919574	2	32	from	liraglutide	357:367	arg1	region					414:419	the locally injected region	393:419	the locally injected region	393:419	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	5	33	theme	liraglutide-loaded	854:871	arg1	TLM					873:875	the liraglutide-loaded TLM	850:875	the liraglutide-loaded TLM	850:875	Daily topical application of the liraglutide-loaded TLM significantly reduced body weight and improved body composition in a mouse model of high-fat diet-induced obesity.					
36919574	0	34	theme	liraglutide-encapsulated	15:38	arg1	TLMs					83:86	TLMs	83:86	TLMs	83:86	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.					
36919574	0	34	theme	liraglutide-encapsulated	15:38	arg1	microneedles					69:80	liraglutide-encapsulated triple layer hyaluronic acid microneedles	15:80	liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs)	15:87	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.					
36919574	5	35	theme	obesity	983:989	arg1	model					952:956	a mouse model	944:956	a mouse model of high-fat diet-induced obesity	944:989	Daily topical application of the liraglutide-loaded TLM significantly reduced body weight and improved body composition in a mouse model of high-fat diet-induced obesity.					
36919574	2	36	theme	low	281:283	arg1	bioavailability					285:299	the low bioavailability	277:299	the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region	277:419	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	4	37	theme	microneedle	662:672	arg1	approaches					674:683	previous anti-obesity microneedle approaches	640:683	previous anti-obesity microneedle approaches	640:683	In contrast to previous anti-obesity microneedle approaches, this TLM is composed of three layers for complete skin insertion, protecting the encapsulated liraglutide from environmental stresses.					
36919574	2	38	theme	injected	405:412	arg1	region					414:419	the locally injected region	393:419	the locally injected region	393:419	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	0	39	theme	hyaluronic	53:62	arg1	TLMs					83:86	TLMs	83:86	TLMs	83:86	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.					
36919574	0	39	theme	hyaluronic	53:62	arg1	microneedles					69:80	liraglutide-encapsulated triple layer hyaluronic acid microneedles	15:80	liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs)	15:87	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.					
36919574	7	40	theme	glucagon-like	1098:1110	arg1	peptide-1					1112:1120	glucagon-like peptide-1	1098:1120	a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection	1096:1252	This novel TLM could promote a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection.					
36919574	3	41	theme	long-term	593:601	arg1	system					617:622	a painless and patient-friendly long-term drug delivery system	561:622	a painless and patient-friendly long-term drug delivery system	561:622	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	41	theme	long-term	593:601	arg1	microneedle					540:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle	472:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM)	472:556	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	4	42	theme	anti-obesity	649:660	arg1	approaches					674:683	previous anti-obesity microneedle approaches	640:683	previous anti-obesity microneedle approaches	640:683	In contrast to previous anti-obesity microneedle approaches, this TLM is composed of three layers for complete skin insertion, protecting the encapsulated liraglutide from environmental stresses.					
36919574	0	43	theme	layer	47:51	arg1	TLMs					83:86	TLMs	83:86	TLMs	83:86	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.					
36919574	0	43	theme	layer	47:51	arg1	microneedles					69:80	liraglutide-encapsulated triple layer hyaluronic acid microneedles	15:80	liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs)	15:87	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.					
36919574	7	44	theme	peptide-1	1112:1120	arg1	system					1135:1140	a glucagon-like peptide-1 drug release system	1096:1140	a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection	1096:1252	This novel TLM could promote a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection.					
36919574	3	45	theme	drug	603:606	arg1	system					617:622	a painless and patient-friendly long-term drug delivery system	561:622	a painless and patient-friendly long-term drug delivery system	561:622	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	45	theme	drug	603:606	arg1	microneedle					540:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle	472:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM)	472:556	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	4	46	theme	previous	640:647	arg1	approaches					674:683	previous anti-obesity microneedle approaches	640:683	previous anti-obesity microneedle approaches	640:683	In contrast to previous anti-obesity microneedle approaches, this TLM is composed of three layers for complete skin insertion, protecting the encapsulated liraglutide from environmental stresses.					
36919574	0	47	theme	microneedles	69:80	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.	0:117	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.					
36919574	5	48	theme	body	924:927	arg1	composition					929:939	improved body composition	915:939	improved body composition	915:939	Daily topical application of the liraglutide-loaded TLM significantly reduced body weight and improved body composition in a mouse model of high-fat diet-induced obesity.					
36919574	7	49	theme	novel	1072:1076	arg1	TLM					1078:1080	This novel TLM	1067:1080	This novel TLM	1067:1080	This novel TLM could promote a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection.					
36919574	2	50	theme	appetite	335:342	arg1	suppressant					344:354	an appetite suppressant	332:354	an appetite suppressant	332:354	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	0	51	theme	acid	64:67	arg1	TLMs					83:86	TLMs	83:86	TLMs	83:86	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.					
36919574	0	51	theme	acid	64:67	arg1	microneedles					69:80	liraglutide-encapsulated triple layer hyaluronic acid microneedles	15:80	liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs)	15:87	Fabrication of liraglutide-encapsulated triple layer hyaluronic acid microneedles (TLMs) for the treatment of obesity.					
36919574	3	52	theme	patient-friendly	576:591	arg1	system					617:622	a painless and patient-friendly long-term drug delivery system	561:622	a painless and patient-friendly long-term drug delivery system	561:622	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	52	theme	patient-friendly	576:591	arg1	microneedle					540:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle	472:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM)	472:556	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	53	theme	hyaluronic	480:489	arg1	system					617:622	a painless and patient-friendly long-term drug delivery system	561:622	a painless and patient-friendly long-term drug delivery system	561:622	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	53	theme	hyaluronic	480:489	arg1	TLM					553:555	TLM	553:555	TLM	553:555	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	53	theme	hyaluronic	480:489	arg1	microneedle					540:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle	472:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM)	472:556	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	7	54	theme	subcutaneous	1231:1242	arg1	injection					1244:1252	subcutaneous injection	1231:1252	subcutaneous injection	1231:1252	This novel TLM could promote a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection.					
36919574	7	55	with	administration	1162:1175	arg1	compliance					1208:1217	relatively higher patient compliance	1182:1217	relatively higher patient compliance	1182:1217	This novel TLM could promote a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection.					
36919574	2	56	from	problems	381:388	arg1	region					414:419	the locally injected region	393:419	the locally injected region	393:419	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	4	57	theme	complete	727:734	arg1	insertion					741:749	complete skin insertion	727:749	complete skin insertion	727:749	In contrast to previous anti-obesity microneedle approaches, this TLM is composed of three layers for complete skin insertion, protecting the encapsulated liraglutide from environmental stresses.					
36919574	4	58	theme	environmental	797:809	arg1	stresses					811:818	environmental stresses	797:818	environmental stresses	797:818	In contrast to previous anti-obesity microneedle approaches, this TLM is composed of three layers for complete skin insertion, protecting the encapsulated liraglutide from environmental stresses.					
36919574	1	59	theme	metabolic	140:148	arg1	worldwide					176:184	prevalent worldwide	166:184	prevalent worldwide	166:184	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36919574	1	59	theme	metabolic	140:148	arg1	disease					150:156	a chronic metabolic disease	130:156	a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans	130:263	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36919574	1	59	theme	metabolic	140:148	arg1	Obesity					119:125	Obesity	119:125	Obesity	119:125	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36919574	5	60	theme	high-fat	961:968	arg1	obesity					983:989	high-fat diet-induced obesity	961:989	high-fat diet-induced obesity	961:989	Daily topical application of the liraglutide-loaded TLM significantly reduced body weight and improved body composition in a mouse model of high-fat diet-induced obesity.					
36919574	3	61	theme	delivery	608:615	arg1	system					617:622	a painless and patient-friendly long-term drug delivery system	561:622	a painless and patient-friendly long-term drug delivery system	561:622	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	3	61	theme	delivery	608:615	arg1	microneedle					540:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle	472:550	a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM)	472:556	In this study, we developed a novel hyaluronic acid-based liraglutide-encapsulated triple-layer microneedle (TLM) as a painless and patient-friendly long-term drug delivery system.					
36919574	1	62	theme	chronic	132:138	arg1	worldwide					176:184	prevalent worldwide	166:184	prevalent worldwide	166:184	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36919574	1	62	theme	chronic	132:138	arg1	disease					150:156	a chronic metabolic disease	130:156	a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans	130:263	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36919574	1	62	theme	chronic	132:138	arg1	Obesity					119:125	Obesity	119:125	Obesity	119:125	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36919574	2	63	theme	problems	381:388	arg1	injection					319:327	subcutaneous injection	306:327	subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region	306:419	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	7	64	theme	patient	1200:1206	arg1	compliance					1208:1217	relatively higher patient compliance	1182:1217	relatively higher patient compliance	1182:1217	This novel TLM could promote a glucagon-like peptide-1 drug release system for long-term daily administration with relatively higher patient compliance compared to subcutaneous injection.					
36919574	2	65	from	region	414:419	arg1	injection					319:327	subcutaneous injection	306:327	subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region	306:419	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	2	66	theme	liraglutide	357:367	arg1	injection					319:327	subcutaneous injection	306:327	subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region	306:419	Currently, the low bioavailability upon subcutaneous injection of an appetite suppressant, liraglutide, and health problems in the locally injected region remain to be overcome.					
36919574	1	67	theme	life	236:239	arg1	longevity					245:253	longevity	245:253	longevity of humans	245:263	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36919574	1	67	theme	life	236:239	arg1	quality					225:231	the quality	221:231	the quality of life	221:239	Obesity is a chronic metabolic disease that is prevalent worldwide, causing complications that affect the quality of life and longevity of humans.					
36008507	0	0	dep	deer	92:95	arg1	elaphus					105:111	Cervus elaphus	98:111	Cervus elaphus	98:111	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	1	1	theme	offspring	207:215	arg1	sex					217:219	the offspring sex	203:219	the offspring sex that will secure higher reproductive output	203:263	Differential maternal allocation theory states that mothers will invest more heavily in the offspring sex that will secure higher reproductive output.					
36008507	5	2	from	sex-dependence	978:991	arg1	protein					1037:1043	protein	1037:1043	protein	1037:1043	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	2	from	sex-dependence	978:991	arg1	density					1023:1029	energy density	1016:1029	energy density	1016:1029	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	2	from	sex-dependence	978:991	arg1	content					1057:1063	lactose content	1049:1063	lactose content	1049:1063	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	2	from	sex-dependence	978:991	arg1	fat					1032:1034	fat	1032:1034	fat	1032:1034	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	2	from	sex-dependence	978:991	arg1	traits					1001:1006	milk traits	996:1006	milk traits (yield, energy density, fat, protein and lactose content)	996:1064	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	2	from	sex-dependence	978:991	arg1	yield					1009:1013	yield	1009:1013	yield	1009:1013	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	3	theme	mother	844:849	arg1	age					851:853	mother age	844:853	mother age	844:853	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	1	4	theme	Differential	115:126	arg1	allocation					137:146	Differential maternal allocation	115:146	Differential maternal allocation	115:146	Differential maternal allocation theory states that mothers will invest more heavily in the offspring sex that will secure higher reproductive output.					
36008507	0	5	theme	calf	73:76	arg1	growth					78:83	calf growth	73:83	calf growth	73:83	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	0	6	from	Effects	0:6	arg1	composition					57:67	composition	57:67	composition	57:67	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	0	6	from	Effects	0:6	arg1	growth					78:83	calf growth	73:83	calf growth	73:83	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	0	6	from	Effects	0:6	arg1	yield					50:54	milk yield	45:54	milk yield	45:54	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	6	7	theme	body	1193:1196	arg1	weight					1198:1203	body weight	1193:1203	body weight	1193:1203	Our findings suggest that maternal allocation responds to offspring energy requirements, which are mainly driven by offspring body weight, and contingent on mother age and weight and previous maternal reproductive effort.					
36008507	5	8	theme	differential	944:955	arg1	sex-dependence					978:991	differential allocation offspring sex-dependence	944:991	differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content)	944:1064	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	6	9	theme	maternal	1093:1100	arg1	allocation					1102:1111	maternal allocation	1093:1111	maternal allocation	1093:1111	Our findings suggest that maternal allocation responds to offspring energy requirements, which are mainly driven by offspring body weight, and contingent on mother age and weight and previous maternal reproductive effort.					
36008507	4	10	theme	little	612:617	arg1	evidence					619:626	little evidence	612:626	little evidence for the differential allocation theory on milk traits	612:680	Previous studies revealed that there was little evidence for the differential allocation theory on milk traits and that most studies lacked proper control for confounding factors.					
36008507	5	11	theme	mother	884:889	arg1	weight					891:896	mother weight	884:896	mother weight	884:896	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	12	theme	energy	1016:1021	arg1	density					1023:1029	energy density	1016:1029	energy density	1016:1029	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	12	theme	energy	1016:1021	arg1	traits					1001:1006	milk traits	996:1006	milk traits (yield, energy density, fat, protein and lactose content)	996:1064	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	4	13	theme	proper	711:716	arg1	control					718:724	proper control	711:724	proper control for confounding factors	711:748	Previous studies revealed that there was little evidence for the differential allocation theory on milk traits and that most studies lacked proper control for confounding factors.					
36008507	3	14	theme	Iberian	464:470	arg1	population					472:481	an Iberian population	461:481	an Iberian population of captive red deer (Cervus elaphus)	461:518	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	5	15	dep	male-biased	808:818	arg1	affected					832:839	affected	832:839	negatively affected by mother age	821:853	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	15	dep	male-biased	808:818	arg1	influenced					870:879	influenced	870:879	positively influenced by mother weight and parity	859:907	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	15	dep	male-biased	808:818	arg1	i					779:779	i	779:779	i	779:779	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	4	16	theme	milk	670:673	arg1	traits					675:680	milk traits	670:680	milk traits	670:680	Previous studies revealed that there was little evidence for the differential allocation theory on milk traits and that most studies lacked proper control for confounding factors.					
36008507	5	17	theme	calf	782:785	arg1	growth					787:792	calf growth	782:792	calf growth	782:792	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	3	18	theme	red	494:496	arg1	deer					498:501	captive red deer	486:501	captive red deer (Cervus elaphus)	486:518	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	1	19	theme	maternal	128:135	arg1	allocation					137:146	Differential maternal allocation	115:146	Differential maternal allocation	115:146	Differential maternal allocation theory states that mothers will invest more heavily in the offspring sex that will secure higher reproductive output.					
36008507	6	20	theme	previous	1250:1257	arg1	effort					1281:1286	previous maternal reproductive effort	1250:1286	previous maternal reproductive effort	1250:1286	Our findings suggest that maternal allocation responds to offspring energy requirements, which are mainly driven by offspring body weight, and contingent on mother age and weight and previous maternal reproductive effort.					
36008507	6	21	theme	maternal	1259:1266	arg1	effort					1281:1286	previous maternal reproductive effort	1250:1286	previous maternal reproductive effort	1250:1286	Our findings suggest that maternal allocation responds to offspring energy requirements, which are mainly driven by offspring body weight, and contingent on mother age and weight and previous maternal reproductive effort.					
36008507	3	22	theme	offspring	380:388	arg1	response					404:411	the offspring sex-dependent response	376:411	the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus)	376:518	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	3	23	theme	deer	498:501	arg1	population					472:481	an Iberian population	461:481	an Iberian population of captive red deer (Cervus elaphus)	461:518	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	3	24	theme	growth	421:426	arg1	response					404:411	the offspring sex-dependent response	376:411	the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus)	376:518	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	3	25	from	age	454:456	arg1	population					472:481	an Iberian population	461:481	an Iberian population of captive red deer (Cervus elaphus)	461:518	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	4	26	theme	most	691:694	arg1	studies					696:702	most studies	691:702	most studies	691:702	Previous studies revealed that there was little evidence for the differential allocation theory on milk traits and that most studies lacked proper control for confounding factors.					
36008507	0	27	theme	age	20:22	arg1	Effects					0:6	Effects	0:6	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).	0:113	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	1	28	theme	higher	238:243	arg1	output					258:263	higher reproductive output	238:263	higher reproductive output	238:263	Differential maternal allocation theory states that mothers will invest more heavily in the offspring sex that will secure higher reproductive output.					
36008507	0	29	theme	deer	92:95	arg1	composition					57:67	composition	57:67	composition	57:67	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	0	29	theme	deer	92:95	arg1	growth					78:83	calf growth	73:83	calf growth	73:83	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	0	29	theme	deer	92:95	arg1	yield					50:54	milk yield	45:54	milk yield	45:54	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	5	30	theme	allocation	957:966	arg1	sex-dependence					978:991	differential allocation offspring sex-dependence	944:991	differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content)	944:1064	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	2	31	theme	Senescence	266:275	arg1	theory					277:282	Senescence theory	266:282	Senescence theory	266:282	Senescence theory is concerned with the gradual deterioration of physiological function with age.					
36008507	3	32	theme	traits	437:442	arg1	response					404:411	the offspring sex-dependent response	376:411	the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus)	376:518	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	0	33	theme	maternal	11:18	arg1	age					20:22	maternal age	11:22	maternal age	11:22	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	1	34	theme	reproductive	245:256	arg1	output					258:263	higher reproductive output	238:263	higher reproductive output	238:263	Differential maternal allocation theory states that mothers will invest more heavily in the offspring sex that will secure higher reproductive output.					
36008507	0	35	theme	red	88:90	arg1	deer					92:95	red deer	88:95	red deer (Cervus elaphus)	88:112	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	4	36	theme	allocation	649:658	arg1	theory					660:665	the differential allocation theory	632:665	the differential allocation theory on milk traits	632:680	Previous studies revealed that there was little evidence for the differential allocation theory on milk traits and that most studies lacked proper control for confounding factors.					
36008507	4	37	theme	differential	636:647	arg1	theory					660:665	the differential allocation theory	632:665	the differential allocation theory on milk traits	632:680	Previous studies revealed that there was little evidence for the differential allocation theory on milk traits and that most studies lacked proper control for confounding factors.					
36008507	0	38	theme	offspring	28:36	arg1	sex					38:40	offspring sex	28:40	offspring sex	28:40	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	3	39	theme	series	541:546	arg1	data					561:564	a 22 year time series longitudinal data	526:564	a 22 year time series longitudinal data set	526:568	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	3	40	theme	calf	416:419	arg1	growth					421:426	calf growth	416:426	calf growth	416:426	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	3	41	theme	sex-dependent	390:402	arg1	response					404:411	the offspring sex-dependent response	376:411	the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus)	376:518	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	3	42	theme	longitudinal	548:559	arg1	data					561:564	a 22 year time series longitudinal data	526:564	a 22 year time series longitudinal data set	526:568	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	3	43	theme	milk	432:435	arg1	traits					437:442	milk traits	432:442	milk traits	432:442	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	6	44	theme	mother	1224:1229	arg1	age					1231:1233	mother age	1224:1233	mother age	1224:1233	Our findings suggest that maternal allocation responds to offspring energy requirements, which are mainly driven by offspring body weight, and contingent on mother age and weight and previous maternal reproductive effort.					
36008507	3	45	theme	22 year	528:534	arg1	series					541:546	a 22 year time series	526:546	a 22 year time series longitudinal data set	526:568	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	2	46	theme	gradual	306:312	arg1	deterioration					314:326	the gradual deterioration	302:326	the gradual deterioration of physiological function with age	302:361	Senescence theory is concerned with the gradual deterioration of physiological function with age.					
36008507	0	47	theme	sex	38:40	arg1	Effects					0:6	Effects	0:6	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).	0:113	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	5	48	theme	offspring	968:976	arg1	sex-dependence					978:991	differential allocation offspring sex-dependence	944:991	differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content)	944:1064	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	3	49	theme	time	536:539	arg1	series					541:546	a 22 year time series	526:546	a 22 year time series longitudinal data set	526:568	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	3	50	dep	deer	498:501	arg1	elaphus					511:517	Cervus elaphus	504:517	Cervus elaphus	504:517	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	6	51	theme	energy	1135:1140	arg1	requirements					1142:1153	offspring energy requirements	1125:1153	offspring energy requirements	1125:1153	Our findings suggest that maternal allocation responds to offspring energy requirements, which are mainly driven by offspring body weight, and contingent on mother age and weight and previous maternal reproductive effort.					
36008507	6	52	theme	offspring	1125:1133	arg1	requirements					1142:1153	offspring energy requirements	1125:1153	offspring energy requirements	1125:1153	Our findings suggest that maternal allocation responds to offspring energy requirements, which are mainly driven by offspring body weight, and contingent on mother age and weight and previous maternal reproductive effort.					
36008507	3	53	theme	mother	447:452	arg1	age					454:456	mother age	447:456	mother age in an Iberian population of captive red deer (Cervus elaphus)	447:518	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	4	54	theme	confounding	730:740	arg1	factors					742:748	confounding factors	730:748	confounding factors	730:748	Previous studies revealed that there was little evidence for the differential allocation theory on milk traits and that most studies lacked proper control for confounding factors.					
36008507	2	55	with	function	345:352	arg1	age					359:361	age	359:361	age	359:361	Senescence theory is concerned with the gradual deterioration of physiological function with age.					
36008507	5	56	dep	traits	1001:1006	arg1	protein					1037:1043	protein	1037:1043	protein	1037:1043	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	56	dep	traits	1001:1006	arg1	density					1023:1029	energy density	1016:1029	energy density	1016:1029	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	56	dep	traits	1001:1006	arg1	content					1057:1063	lactose content	1049:1063	lactose content	1049:1063	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	56	dep	traits	1001:1006	arg1	fat					1032:1034	fat	1032:1034	fat	1032:1034	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	56	dep	traits	1001:1006	arg1	traits					1001:1006	milk traits	996:1006	milk traits (yield, energy density, fat, protein and lactose content)	996:1064	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	56	dep	traits	1001:1006	arg1	yield					1009:1013	yield	1009:1013	yield	1009:1013	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	3	57	theme	captive	486:492	arg1	deer					498:501	captive red deer	486:501	captive red deer (Cervus elaphus)	486:518	We analysed the offspring sex-dependent response of calf growth and milk traits to mother age in an Iberian population of captive red deer (Cervus elaphus) using a 22 year time series longitudinal data set.					
36008507	5	58	theme	milk	996:999	arg1	protein					1037:1043	protein	1037:1043	protein	1037:1043	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	58	theme	milk	996:999	arg1	density					1023:1029	energy density	1016:1029	energy density	1016:1029	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	58	theme	milk	996:999	arg1	content					1057:1063	lactose content	1049:1063	lactose content	1049:1063	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	58	theme	milk	996:999	arg1	fat					1032:1034	fat	1032:1034	fat	1032:1034	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	58	theme	milk	996:999	arg1	traits					1001:1006	milk traits	996:1006	milk traits (yield, energy density, fat, protein and lactose content)	996:1064	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	58	theme	milk	996:999	arg1	yield					1009:1013	yield	1009:1013	yield	1009:1013	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	59	theme	lactose	1049:1055	arg1	content					1057:1063	lactose content	1049:1063	lactose content	1049:1063	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	59	theme	lactose	1049:1055	arg1	traits					1001:1006	milk traits	996:1006	milk traits (yield, energy density, fat, protein and lactose content)	996:1064	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	5	60	dep	was	925:927	arg1	ii					915:916	ii	915:916	ii	915:916	Our results indicated that (i) calf growth was offspring male-biased, negatively affected by mother age and positively influenced by mother weight and parity, and (ii) there was no support for differential allocation offspring sex-dependence in milk traits (yield, energy density, fat, protein and lactose content).					
36008507	2	61	theme	function	345:352	arg1	deterioration					314:326	the gradual deterioration	302:326	the gradual deterioration of physiological function with age	302:361	Senescence theory is concerned with the gradual deterioration of physiological function with age.					
36008507	4	62	theme	Previous	571:578	arg1	studies					580:586	Previous studies	571:586	Previous studies	571:586	Previous studies revealed that there was little evidence for the differential allocation theory on milk traits and that most studies lacked proper control for confounding factors.					
36008507	0	63	theme	milk	45:48	arg1	yield					50:54	milk yield	45:54	milk yield	45:54	Effects of maternal age and offspring sex on milk yield, composition and calf growth of red deer (Cervus elaphus).					
36008507	2	64	theme	physiological	331:343	arg1	function					345:352	physiological function	331:352	physiological function with age	331:361	Senescence theory is concerned with the gradual deterioration of physiological function with age.					
36008507	4	65	from	theory	660:665	arg1	traits					675:680	milk traits	670:680	milk traits	670:680	Previous studies revealed that there was little evidence for the differential allocation theory on milk traits and that most studies lacked proper control for confounding factors.					
36008507	6	66	theme	reproductive	1268:1279	arg1	effort					1281:1286	previous maternal reproductive effort	1250:1286	previous maternal reproductive effort	1250:1286	Our findings suggest that maternal allocation responds to offspring energy requirements, which are mainly driven by offspring body weight, and contingent on mother age and weight and previous maternal reproductive effort.					
37149710	9	0	theme	protective	1135:1144	arg1	association					1146:1156	a borderline protective association	1122:1156	a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1122:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	7	1	theme	Linear	887:892	arg1	trends					894:899	Linear trends	887:899	Linear trends	887:899	Linear trends were examined based on a Wald test.					
37149710	6	2	theme	%	824:824	arg1	gain					806:809	weight gain	799:809	weight gain of at least 5%	799:824	Two-staged pooling was applied to derive relative risks across cohorts for weight gain of at least 5% by exposure variable intake quintiles in a 7-year follow-up.					
37149710	2	3	theme	dietary	292:298	arg1	carbohydrates					277:289	total carbohydrates	271:289	total carbohydrates	271:289	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	2	3	theme	dietary	292:298	arg1	fiber					300:304	dietary fiber	292:304	dietary fiber	292:304	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	8	4	theme	total	992:996	arg1	carbohydrate					998:1009	total carbohydrate	992:1009	total carbohydrate	992:1009	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	6	5	theme	exposure	829:836	arg1	quintiles					854:862	exposure variable intake quintiles	829:862	exposure variable intake quintiles in a 7-year follow-up	829:884	Two-staged pooling was applied to derive relative risks across cohorts for weight gain of at least 5% by exposure variable intake quintiles in a 7-year follow-up.					
37149710	5	6	theme	Anthropometric	648:661	arg1	measurements					663:674	Anthropometric measurements	648:674	Anthropometric measurements	648:674	Anthropometric measurements were collected according to standard protocols.					
37149710	9	7	theme	weight	1175:1180	arg1	gain					1182:1185	weight gain	1175:1185	weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1175:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	8	8	theme	fiber	1020:1024	arg1	intakes					981:987	intakes	981:987	intakes of total carbohydrate, dietary fiber, total sugar or sucrose	981:1048	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	8	8	theme	fiber	1020:1024	arg1	risk					1058:1061	the risk	1054:1061	the risk of weight gain of at least 5%	1054:1091	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	3	9	from	cohorts	491:497	arg1	aged					436:439	aged	436:439	aged	436:439	METHODS Our data comprised 8327 adults aged 25-70 years in three population-based prospective cohorts.					
37149710	9	10	from	gain	1182:1185	arg1	participants					1190:1201	participants	1190:1201	participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1190:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	1	11	from	research	209:216	arg1	subcategories					234:246	carbohydrate subcategories	221:246	carbohydrate subcategories	221:246	BACKGROUND The role of carbohydrate quantity and quality in weight gain remains unsolved, and research on carbohydrate subcategories is scarce.					
37149710	6	12	from	quintiles	854:862	arg1	follow-up					876:884	a 7-year follow-up	867:884	a 7-year follow-up	867:884	Two-staged pooling was applied to derive relative risks across cohorts for weight gain of at least 5% by exposure variable intake quintiles in a 7-year follow-up.					
37149710	5	13	theme	standard	704:711	arg1	protocols					713:721	standard protocols	704:721	standard protocols	704:721	Anthropometric measurements were collected according to standard protocols.					
37149710	9	14	from	risk	1167:1170	arg1	participants					1190:1201	participants	1190:1201	participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1190:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	1	15	theme	carbohydrate	138:149	arg1	quantity					151:158	carbohydrate quantity	138:158	carbohydrate quantity	138:158	BACKGROUND The role of carbohydrate quantity and quality in weight gain remains unsolved, and research on carbohydrate subcategories is scarce.					
37149710	0	16	theme	pooled	64:69	arg1	analysis					71:78	a pooled analysis	62:78	a pooled analysis of three population-based studies	62:112	Different carbohydrate exposures and weight gain-results from a pooled analysis of three population-based studies.					
37149710	9	17	with	participants	1298:1309	arg1	decrease					1321:1328	≥10% decrease	1316:1328	≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1316:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	8	18	theme	total	1027:1031	arg1	sugar					1033:1037	total sugar	1027:1037	total sugar	1027:1037	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	11	19	theme	weight	1668:1673	arg1	gain					1675:1678	weight gain	1668:1678	weight gain	1668:1678	CONCLUSIONS Our findings do not support an association between carbohydrate intake and weight gain.					
37149710	0	20	theme	population-based	89:104	arg1	studies					106:112	three population-based studies	83:112	three population-based studies	83:112	Different carbohydrate exposures and weight gain-results from a pooled analysis of three population-based studies.					
37149710	9	21	theme	baseline	1434:1441	arg1	weight					1443:1448	baseline weight	1434:1448	baseline weight	1434:1448	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	12	22	theme	weight	1796:1801	arg1	change					1803:1808	weight change	1796:1808	weight change	1796:1808	However, the results suggested that concurrent changes in carbohydrate intake might be an important determinant of weight change and should be further examined in future studies.					
37149710	6	23	theme	Two-staged	724:733	arg1	pooling					735:741	Two-staged pooling	724:741	Two-staged pooling	724:741	Two-staged pooling was applied to derive relative risks across cohorts for weight gain of at least 5% by exposure variable intake quintiles in a 7-year follow-up.					
37149710	2	24	theme	weight	366:371	arg1	gain					373:376	weight gain	366:376	weight gain in Finnish adults	366:394	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	0	25	theme	Different	0:8	arg1	exposures					23:31	Different carbohydrate exposures	0:31	Different carbohydrate exposures	0:31	Different carbohydrate exposures and weight gain-results from a pooled analysis of three population-based studies.					
37149710	4	26	theme	food	533:536	arg1	questionnaire					548:560	a validated food frequency questionnaire	521:560	a validated food frequency questionnaire	521:560	Diet was assessed by a validated food frequency questionnaire and nutrient intakes were calculated utilizing the Finnish Food Composition Database.					
37149710	8	27	theme	weight	1066:1071	arg1	gain					1073:1076	weight gain	1066:1076	weight gain of at least 5%	1066:1091	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	9	28	dep	RR	1375:1376	arg1	CI					1388:1389	95% CI 0.61-1.00	1384:1399	RR 0.78; 95% CI 0.61-1.00	1375:1399	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	9	29	with	participants	1190:1201	arg1	obesity					1208:1214	obesity	1208:1214	obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile)	1208:1274	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	9	29	with	participants	1190:1201	arg1	intake					1288:1293	sucrose intake	1280:1293	sucrose intake	1280:1293	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	6	30	theme	7-year	869:874	arg1	follow-up					876:884	a 7-year follow-up	867:884	a 7-year follow-up	867:884	Two-staged pooling was applied to derive relative risks across cohorts for weight gain of at least 5% by exposure variable intake quintiles in a 7-year follow-up.					
37149710	12	31	theme	concurrent	1717:1726	arg1	determinant					1781:1791	an important determinant	1768:1791	an important determinant of weight change	1768:1808	However, the results suggested that concurrent changes in carbohydrate intake might be an important determinant of weight change and should be further examined in future studies.					
37149710	12	31	theme	concurrent	1717:1726	arg1	changes					1728:1734	concurrent changes	1717:1734	concurrent changes in carbohydrate intake	1717:1757	However, the results suggested that concurrent changes in carbohydrate intake might be an important determinant of weight change and should be further examined in future studies.					
37149710	3	32	from	aged	436:439	arg1	cohorts					491:497	three population-based prospective cohorts	456:497	three population-based prospective cohorts	456:497	METHODS Our data comprised 8327 adults aged 25-70 years in three population-based prospective cohorts.					
37149710	3	33	theme	population-based	462:477	arg1	cohorts					491:497	three population-based prospective cohorts	456:497	three population-based prospective cohorts	456:497	METHODS Our data comprised 8327 adults aged 25-70 years in three population-based prospective cohorts.					
37149710	9	34	contain	had	1118:1120	arg2	association					1146:1156	a borderline protective association	1122:1156	a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1122:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	9	34	contain	had	1118:1120	arg1	intake					1111:1116	total sugar intake	1099:1116	total sugar intake	1099:1116	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	12	35	theme	carbohydrate	1739:1750	arg1	intake					1752:1757	carbohydrate intake	1739:1757	carbohydrate intake	1739:1757	However, the results suggested that concurrent changes in carbohydrate intake might be an important determinant of weight change and should be further examined in future studies.					
37149710	8	36	theme	No	945:946	arg1	association					948:958	RESULTS No association	937:958	RESULTS No association	937:958	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	12	37	from	changes	1728:1734	arg1	intake					1752:1757	carbohydrate intake	1739:1757	carbohydrate intake	1739:1757	However, the results suggested that concurrent changes in carbohydrate intake might be an important determinant of weight change and should be further examined in future studies.					
37149710	9	38	theme	sugar	1105:1109	arg1	intake					1111:1116	total sugar intake	1099:1116	total sugar intake	1099:1116	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	2	39	theme	sucrose	324:330	arg1	intake					332:337	sucrose intake	324:337	sucrose intake	324:337	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	2	39	theme	sucrose	324:330	arg1	carbohydrates					277:289	total carbohydrates	271:289	total carbohydrates	271:289	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	7	40	theme	Wald	926:929	arg1	test					931:934	a Wald test	924:934	a Wald test	924:934	Linear trends were examined based on a Wald test.					
37149710	9	41	theme	≥10	1316:1318	arg1	decrease					1321:1328	≥10% decrease	1316:1328	≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1316:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	8	42	theme	%	1091:1091	arg1	gain					1073:1076	weight gain	1066:1076	weight gain of at least 5%	1066:1091	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	6	43	theme	intake	847:852	arg1	quintiles					854:862	exposure variable intake quintiles	829:862	exposure variable intake quintiles in a 7-year follow-up	829:884	Two-staged pooling was applied to derive relative risks across cohorts for weight gain of at least 5% by exposure variable intake quintiles in a 7-year follow-up.					
37149710	2	44	from	adults	389:394	arg1	risk					358:361	the risk	354:361	the risk of weight gain in Finnish adults	354:394	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	1	45	dep	BACKGROUND	115:124	arg1	remains					187:193	remains	187:193	remains unsolved	187:202	BACKGROUND The role of carbohydrate quantity and quality in weight gain remains unsolved, and research on carbohydrate subcategories is scarce.					
37149710	1	45	dep	BACKGROUND	115:124	arg1	scarce					251:256	scarce	251:256	scarce	251:256	BACKGROUND The role of carbohydrate quantity and quality in weight gain remains unsolved, and research on carbohydrate subcategories is scarce.					
37149710	9	46	dep	obesity	1208:1214	arg1	RR					1217:1218	RR	1217:1218	RR	1217:1218	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	3	47	theme	aged	436:439	arg1	adults					429:434	8327 adults	424:434	8327 adults aged 25-70 years in three population-based prospective cohorts	424:497	METHODS Our data comprised 8327 adults aged 25-70 years in three population-based prospective cohorts.					
37149710	6	48	theme	relative	765:772	arg1	risks					774:778	relative risks	765:778	relative risks across cohorts for weight gain of at least 5%	765:824	Two-staged pooling was applied to derive relative risks across cohorts for weight gain of at least 5% by exposure variable intake quintiles in a 7-year follow-up.					
37149710	4	49	theme	Finnish	613:619	arg1	Database					638:645	the Finnish Food Composition Database	609:645	the Finnish Food Composition Database	609:645	Diet was assessed by a validated food frequency questionnaire and nutrient intakes were calculated utilizing the Finnish Food Composition Database.					
37149710	9	50	theme	borderline	1124:1133	arg1	association					1146:1156	a borderline protective association	1122:1156	a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1122:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	9	51	theme	carbohydrate	1333:1344	arg1	intake					1346:1351	carbohydrate intake	1333:1351	carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1333:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	4	52	theme	nutrient	566:573	arg1	intakes					575:581	nutrient intakes	566:581	nutrient intakes	566:581	Diet was assessed by a validated food frequency questionnaire and nutrient intakes were calculated utilizing the Finnish Food Composition Database.					
37149710	9	53	from	obesity	1208:1214	arg1	participants					1298:1309	participants	1298:1309	participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1298:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	8	54	theme	carbohydrate	998:1009	arg1	intakes					981:987	intakes	981:987	intakes of total carbohydrate, dietary fiber, total sugar or sucrose	981:1048	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	8	54	theme	carbohydrate	998:1009	arg1	risk					1058:1061	the risk	1054:1061	the risk of weight gain of at least 5%	1054:1091	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	9	55	dep	RR	1217:1218	arg1	CI					1230:1231	95% CI 0.40-1.00	1226:1241	95% CI 0.40-1.00 for highest vs. lowest quintile	1226:1273	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	1	56	theme	weight	175:180	arg1	gain					182:185	weight gain	175:185	weight gain	175:185	BACKGROUND The role of carbohydrate quantity and quality in weight gain remains unsolved, and research on carbohydrate subcategories is scarce.					
37149710	9	57	from	participants	1190:1201	arg1	risk					1167:1170	the risk	1163:1170	the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1163:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	8	58	theme	dietary	1012:1018	arg1	fiber					1020:1024	dietary fiber	1012:1024	dietary fiber	1012:1024	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	2	59	from	risk	358:361	arg1	adults					389:394	Finnish adults	381:394	Finnish adults	381:394	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	9	60	theme	gain	1182:1185	arg1	risk					1167:1170	the risk	1163:1170	the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1163:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	2	61	theme	total	307:311	arg1	carbohydrates					277:289	total carbohydrates	271:289	total carbohydrates	271:289	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	2	61	theme	total	307:311	arg1	sugar					313:317	total sugar	307:317	total sugar	307:317	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	4	62	theme	Composition	626:636	arg1	Database					638:645	the Finnish Food Composition Database	609:645	the Finnish Food Composition Database	609:645	Diet was assessed by a validated food frequency questionnaire and nutrient intakes were calculated utilizing the Finnish Food Composition Database.					
37149710	10	63	theme	Further	1509:1515	arg1	adjustment					1517:1526	Further adjustment	1509:1526	Further adjustment for fruit consumption	1509:1548	Further adjustment for fruit consumption strengthened the associations.					
37149710	9	64	theme	%	1386:1386	arg1	CI					1388:1389	95% CI 0.61-1.00	1384:1399	RR 0.78; 95% CI 0.61-1.00	1375:1399	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	12	65	theme	future	1844:1849	arg1	studies					1851:1857	future studies	1844:1857	future studies	1844:1857	However, the results suggested that concurrent changes in carbohydrate intake might be an important determinant of weight change and should be further examined in future studies.					
37149710	9	66	from	intake	1288:1293	arg1	participants					1298:1309	participants	1298:1309	participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1298:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	2	67	theme	total	271:275	arg1	intake					332:337	sucrose intake	324:337	sucrose intake	324:337	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	2	67	theme	total	271:275	arg1	carbohydrates					277:289	total carbohydrates	271:289	total carbohydrates	271:289	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	2	67	theme	total	271:275	arg1	fiber					300:304	dietary fiber	292:304	dietary fiber	292:304	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	2	67	theme	total	271:275	arg1	sugar					313:317	total sugar	307:317	total sugar	307:317	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	8	68	theme	RESULTS	937:943	arg1	association					948:958	RESULTS No association	937:958	RESULTS No association	937:958	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	1	69	theme	quantity	151:158	arg1	role					130:133	The role	126:133	The role of carbohydrate quantity and quality in weight gain	126:185	BACKGROUND The role of carbohydrate quantity and quality in weight gain remains unsolved, and research on carbohydrate subcategories is scarce.					
37149710	1	70	theme	carbohydrate	221:232	arg1	subcategories					234:246	carbohydrate subcategories	221:246	carbohydrate subcategories	221:246	BACKGROUND The role of carbohydrate quantity and quality in weight gain remains unsolved, and research on carbohydrate subcategories is scarce.					
37149710	11	71	theme	carbohydrate	1644:1655	arg1	intake					1657:1662	carbohydrate intake	1644:1662	carbohydrate intake	1644:1662	CONCLUSIONS Our findings do not support an association between carbohydrate intake and weight gain.					
37149710	9	72	theme	%	1228:1228	arg1	CI					1230:1231	95% CI 0.40-1.00	1226:1241	95% CI 0.40-1.00 for highest vs. lowest quintile	1226:1273	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	8	73	theme	sugar	1033:1037	arg1	intakes					981:987	intakes	981:987	intakes of total carbohydrate, dietary fiber, total sugar or sucrose	981:1048	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	8	73	theme	sugar	1033:1037	arg1	risk					1058:1061	the risk	1054:1061	the risk of weight gain of at least 5%	1054:1091	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	1	74	theme	quality	164:170	arg1	role					130:133	The role	126:133	The role of carbohydrate quantity and quality in weight gain	126:185	BACKGROUND The role of carbohydrate quantity and quality in weight gain remains unsolved, and research on carbohydrate subcategories is scarce.					
37149710	0	75	theme	studies	106:112	arg1	analysis					71:78	a pooled analysis	62:78	a pooled analysis of three population-based studies	62:112	Different carbohydrate exposures and weight gain-results from a pooled analysis of three population-based studies.					
37149710	8	76	theme	sucrose	1042:1048	arg1	intakes					981:987	intakes	981:987	intakes of total carbohydrate, dietary fiber, total sugar or sucrose	981:1048	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	8	76	theme	sucrose	1042:1048	arg1	risk					1058:1061	the risk	1054:1061	the risk of weight gain of at least 5%	1054:1091	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	9	77	theme	highest	1247:1253	arg1	quintile					1266:1273	highest vs. lowest quintile	1247:1273	quintile	1266:1273	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	12	78	theme	change	1803:1808	arg1	changes					1728:1734	concurrent changes	1717:1734	concurrent changes in carbohydrate intake	1717:1757	However, the results suggested that concurrent changes in carbohydrate intake might be an important determinant of weight change and should be further examined in future studies.					
37149710	12	78	theme	change	1803:1808	arg1	determinant					1781:1791	an important determinant	1768:1791	an important determinant of weight change	1768:1808	However, the results suggested that concurrent changes in carbohydrate intake might be an important determinant of weight change and should be further examined in future studies.					
37149710	2	79	theme	gain	373:376	arg1	risk					358:361	the risk	354:361	the risk of weight gain in Finnish adults	354:394	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	0	80	theme	carbohydrate	10:21	arg1	exposures					23:31	Different carbohydrate exposures	0:31	Different carbohydrate exposures	0:31	Different carbohydrate exposures and weight gain-results from a pooled analysis of three population-based studies.					
37149710	9	81	theme	lowest	1259:1264	arg1	quintile					1266:1273	highest vs. lowest quintile	1247:1273	quintile	1266:1273	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	4	82	theme	frequency	538:546	arg1	questionnaire					548:560	a validated food frequency questionnaire	521:560	a validated food frequency questionnaire	521:560	Diet was assessed by a validated food frequency questionnaire and nutrient intakes were calculated utilizing the Finnish Food Composition Database.					
37149710	3	83	dep	METHODS	397:403	arg1	comprised					414:422	comprised	414:422	comprised 8327 adults aged 25-70 years in three population-based prospective cohorts	414:497	METHODS Our data comprised 8327 adults aged 25-70 years in three population-based prospective cohorts.					
37149710	8	84	theme	gain	1073:1076	arg1	intakes					981:987	intakes	981:987	intakes of total carbohydrate, dietary fiber, total sugar or sucrose	981:1048	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	8	84	theme	gain	1073:1076	arg1	risk					1058:1061	the risk	1054:1061	the risk of weight gain of at least 5%	1054:1091	RESULTS No association was observed between intakes of total carbohydrate, dietary fiber, total sugar or sucrose and the risk of weight gain of at least 5%.					
37149710	1	85	from	role	130:133	arg1	gain					182:185	weight gain	175:185	weight gain	175:185	BACKGROUND The role of carbohydrate quantity and quality in weight gain remains unsolved, and research on carbohydrate subcategories is scarce.					
37149710	9	86	theme	sucrose	1280:1286	arg1	intake					1288:1293	sucrose intake	1280:1293	sucrose intake	1280:1293	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	9	87	theme	physical	1471:1478	arg1	activity					1480:1487	physical activity	1471:1487	physical activity	1471:1487	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	6	88	theme	weight	799:804	arg1	gain					806:809	weight gain	799:809	weight gain of at least 5%	799:824	Two-staged pooling was applied to derive relative risks across cohorts for weight gain of at least 5% by exposure variable intake quintiles in a 7-year follow-up.					
37149710	2	89	theme	Finnish	381:387	arg1	adults					389:394	Finnish adults	381:394	Finnish adults	381:394	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	9	90	from	decrease	1321:1328	arg1	intake					1346:1351	carbohydrate intake	1333:1351	carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1333:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	4	91	theme	validated	523:531	arg1	questionnaire					548:560	a validated food frequency questionnaire	521:560	a validated food frequency questionnaire	521:560	Diet was assessed by a validated food frequency questionnaire and nutrient intakes were calculated utilizing the Finnish Food Composition Database.					
37149710	9	92	theme	total	1099:1103	arg1	intake					1111:1116	total sugar intake	1099:1116	total sugar intake	1099:1116	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	3	93	theme	prospective	479:489	arg1	cohorts					491:497	three population-based prospective cohorts	456:497	three population-based prospective cohorts	456:497	METHODS Our data comprised 8327 adults aged 25-70 years in three population-based prospective cohorts.					
37149710	9	94	with	association	1146:1156	arg1	risk					1167:1170	the risk	1163:1170	the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1163:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	12	95	theme	important	1771:1779	arg1	changes					1728:1734	concurrent changes	1717:1734	concurrent changes in carbohydrate intake	1717:1757	However, the results suggested that concurrent changes in carbohydrate intake might be an important determinant of weight change and should be further examined in future studies.					
37149710	12	95	theme	important	1771:1779	arg1	determinant					1781:1791	an important determinant	1768:1791	an important determinant of weight change	1768:1808	However, the results suggested that concurrent changes in carbohydrate intake might be an important determinant of weight change and should be further examined in future studies.					
37149710	9	96	theme	energy	1494:1499	arg1	intake					1501:1506	energy intake	1494:1506	energy intake	1494:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	2	97	from	gain	373:376	arg1	adults					389:394	Finnish adults	381:394	Finnish adults	381:394	We examined total carbohydrates, dietary fiber, total sugar, and sucrose intake in relation to the risk of weight gain in Finnish adults.					
37149710	11	98	dep	CONCLUSIONS	1581:1591	arg1	support					1613:1619	support	1613:1619	do not support an association between carbohydrate intake and weight gain	1606:1678	CONCLUSIONS Our findings do not support an association between carbohydrate intake and weight gain.					
37149710	9	99	theme	%	1319:1319	arg1	decrease					1321:1328	≥10% decrease	1316:1328	≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake	1316:1506	Yet, total sugar intake had a borderline protective association with the risk of weight gain in participants with obesity (RR 0.63; 95% CI 0.40-1.00 for highest vs. lowest quintile) and sucrose intake in participants with ≥10% decrease in carbohydrate intake during the follow-up (RR 0.78; 95% CI 0.61-1.00) after adjustments for sex, age, baseline weight, education, smoking, physical activity, and energy intake.					
37149710	6	100	theme	variable	838:845	arg1	quintiles					854:862	exposure variable intake quintiles	829:862	exposure variable intake quintiles in a 7-year follow-up	829:884	Two-staged pooling was applied to derive relative risks across cohorts for weight gain of at least 5% by exposure variable intake quintiles in a 7-year follow-up.					
37149710	10	101	theme	fruit	1532:1536	arg1	consumption					1538:1548	fruit consumption	1532:1548	fruit consumption	1532:1548	Further adjustment for fruit consumption strengthened the associations.					
37149710	4	102	theme	Food	621:624	arg1	Database					638:645	the Finnish Food Composition Database	609:645	the Finnish Food Composition Database	609:645	Diet was assessed by a validated food frequency questionnaire and nutrient intakes were calculated utilizing the Finnish Food Composition Database.					
35086703	2	0	theme	design	564:569	arg1	experiment					571:580	a 10-wk randomized complete block design experiment	530:580	a 10-wk randomized complete block design experiment with 16 cows per treatment	530:607	Forty-eight Holstein cows (28 primiparous and 20 multiparous) were enrolled in a 10-wk randomized complete block design experiment with 16 cows per treatment.					
35086703	12	1	theme	ECM	2026:2028	arg1	efficiency					2035:2044	ECM feed efficiency	2026:2044	ECM feed efficiency	2026:2044	Overall, decreasing dietary starch concentration by 26% decreased dry matter intake, milk, and ECM yields, but ECM feed efficiency was not different among treatments.					
35086703	1	2	theme	normal-starch	406:418	arg1	control					441:447	i.e., positive control	426:447	control	441:447	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	2	theme	normal-starch	406:418	arg1	diet					420:423	a normal-starch diet	404:423	a normal-starch diet (i.e., positive control)	404:448	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	8	3	theme	RSD	1416:1418	arg1	treatments					1420:1429	RSD treatments	1416:1429	RSD treatments	1416:1429	Total-tract digestibility of NDF tended to increase by RSD treatments.					
35086703	2	4	theme	complete	549:556	arg1	block					558:562	a 10-wk randomized complete block	530:562	a 10-wk randomized complete block design experiment with 16 cows per treatment	530:607	Forty-eight Holstein cows (28 primiparous and 20 multiparous) were enrolled in a 10-wk randomized complete block design experiment with 16 cows per treatment.					
35086703	8	5	theme	Total-tract	1361:1371	arg1	digestibility					1373:1385	Total-tract digestibility	1361:1385	Total-tract digestibility of NDF	1361:1392	Total-tract digestibility of NDF tended to increase by RSD treatments.					
35086703	1	6	theme	total-tract	248:258	arg1	digestibility					260:272	total-tract digestibility	248:272	total-tract digestibility of nutrients	248:285	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	11	7	theme	acids	1831:1835	arg1	concentrations					1799:1812	decreased concentrations	1789:1812	decreased concentrations of de novo fatty acids	1789:1835	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	4	8	theme	fibrolytic	922:931	arg1	activities					933:942	amylolytic and fibrolytic activities	907:942	amylolytic and fibrolytic activities	907:942	The EEP contained amylolytic and fibrolytic activities and was top-dressed on the total mixed ration at the time of feeding.					
35086703	3	9	theme	%	683:683	arg1	basis					696:700	% dry matter basis	683:700	% dry matter basis	683:700	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	6	10	theme	milk	1241:1244	arg1	fat					1246:1248	milk fat	1241:1248	milk fat	1241:1248	Concentrations, but not yields, of milk fat and total solids were increased by RSD.					
35086703	1	11	theme	supplementation	175:189	arg1	effects					164:170	the effects	160:170	the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control)	160:448	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	11	12	dep	de	1817:1818	arg1	novo					1820:1823	novo	1820:1823	novo	1820:1823	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	3	13	dep	%	707:707	arg1	basis					696:700	% dry matter basis	683:700	% dry matter basis	683:700	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	9	14	theme	Plasma	1432:1437	arg1	concentrations					1442:1455	Plasma AA concentrations	1432:1455	Plasma AA concentrations	1432:1455	Plasma AA concentrations were not affected by treatment, except that of 3-methylhistidine was increased by ENZ, compared with RSD.					
35086703	4	15	theme	amylolytic	907:916	arg1	activities					933:942	amylolytic and fibrolytic activities	907:942	amylolytic and fibrolytic activities	907:942	The EEP contained amylolytic and fibrolytic activities and was top-dressed on the total mixed ration at the time of feeding.					
35086703	1	16	from	performance	235:245	arg1	cows					359:362	lactating dairy cows	343:362	lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control)	343:448	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	17	from	composition	328:338	arg1	cows					359:362	lactating dairy cows	343:362	lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control)	343:448	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	3	18	dep	diet	776:779	arg1	2					758:758	2	758:758	2	758:758	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	1	19	theme	enzyme	207:212	arg1	EEP					227:229	EEP	227:229	EEP	227:229	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	19	theme	enzyme	207:212	arg1	preparation					214:224	an exogenous enzyme preparation	194:224	an exogenous enzyme preparation (EEP)	194:230	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	2	20	theme	10-wk	532:536	arg1	block					558:562	a 10-wk randomized complete block	530:562	a 10-wk randomized complete block design experiment with 16 cows per treatment	530:607	Forty-eight Holstein cows (28 primiparous and 20 multiparous) were enrolled in a 10-wk randomized complete block design experiment with 16 cows per treatment.					
35086703	4	21	theme	mixed	977:981	arg1	ration					983:988	the total mixed ration	967:988	the total mixed ration	967:988	The EEP contained amylolytic and fibrolytic activities and was top-dressed on the total mixed ration at the time of feeding.					
35086703	3	22	theme	matter	689:694	arg1	basis					696:700	% dry matter basis	683:700	% dry matter basis	683:700	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	11	23	theme	decreased	1789:1797	arg1	concentrations					1799:1812	decreased concentrations	1789:1812	decreased concentrations of de novo fatty acids	1789:1835	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	11	24	contain	had	1785:1787	arg1	Cows					1758:1761	Cows	1758:1761	Cows on the RSD treatments	1758:1783	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	11	24	contain	had	1785:1787	arg2	concentrations					1799:1812	decreased concentrations	1789:1812	decreased concentrations of de novo fatty acids	1789:1835	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	5	25	theme	milk	1070:1073	arg1	yields					1107:1112	dry matter intake and milk and energy-corrected milk (ECM) yields	1048:1112	dry matter intake and milk and energy-corrected milk (ECM) yields	1048:1112	Compared with normal-starch diet, dry matter intake and milk and energy-corrected milk (ECM) yields were lower (on average by 7.1, 9.5, and 7.2%, respectively) for cows on the RSD treatments.					
35086703	7	26	theme	feed	1312:1315	arg1	efficiency					1317:1326	Energy-corrected milk feed efficiency	1290:1326	Energy-corrected milk feed efficiency	1290:1326	Energy-corrected milk feed efficiency did not differ among treatments.					
35086703	5	27	theme	energy-corrected	1079:1094	arg1	milk					1096:1099	energy-corrected milk	1079:1099	energy-corrected milk	1079:1099	Compared with normal-starch diet, dry matter intake and milk and energy-corrected milk (ECM) yields were lower (on average by 7.1, 9.5, and 7.2%, respectively) for cows on the RSD treatments.					
35086703	3	28	theme	%	707:707	arg1	starch					709:714	(% dry matter basis) 24.8% starch	682:714	(% dry matter basis) 24.8% starch	682:714	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	7	29	theme	Energy-corrected	1290:1305	arg1	efficiency					1317:1326	Energy-corrected milk feed efficiency	1290:1326	Energy-corrected milk feed efficiency	1290:1326	Energy-corrected milk feed efficiency did not differ among treatments.					
35086703	11	30	theme	acids	1900:1904	arg1	concentrations					1866:1879	increased concentrations	1856:1879	increased concentrations of preformed fatty acids in milk	1856:1912	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	5	31	theme	dry	1048:1050	arg1	intake					1059:1064	dry matter intake	1048:1064	dry matter intake	1048:1064	Compared with normal-starch diet, dry matter intake and milk and energy-corrected milk (ECM) yields were lower (on average by 7.1, 9.5, and 7.2%, respectively) for cows on the RSD treatments.					
35086703	3	32	theme	detergent	734:742	arg1	NDF					751:753	NDF	751:753	NDF	751:753	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	3	32	theme	detergent	734:742	arg1	fiber					744:748	33.0% neutral detergent fiber	720:748	33.0% neutral detergent fiber (NDF)	720:754	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	1	33	theme	fatty	316:320	arg1	composition					328:338	milk fatty acids composition	311:338	milk fatty acids composition	311:338	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	12	34	theme	dry	1981:1983	arg1	intake					1992:1997	dry matter intake	1981:1997	dry matter intake	1981:1997	Overall, decreasing dietary starch concentration by 26% decreased dry matter intake, milk, and ECM yields, but ECM feed efficiency was not different among treatments.					
35086703	5	35	theme	intake	1059:1064	arg1	yields					1107:1112	dry matter intake and milk and energy-corrected milk (ECM) yields	1048:1112	dry matter intake and milk and energy-corrected milk (ECM) yields	1048:1112	Compared with normal-starch diet, dry matter intake and milk and energy-corrected milk (ECM) yields were lower (on average by 7.1, 9.5, and 7.2%, respectively) for cows on the RSD treatments.					
35086703	3	36	theme	%	724:724	arg1	NDF					751:753	NDF	751:753	NDF	751:753	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	3	36	theme	%	724:724	arg1	fiber					744:748	33.0% neutral detergent fiber	720:748	33.0% neutral detergent fiber (NDF)	720:754	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	0	37	theme	exogenous	83:91	arg1	preparation					100:110	an exogenous enzyme preparation	80:110	an exogenous enzyme preparation	80:110	Performance of dairy cows fed normal- or reduced-starch diets supplemented with an exogenous enzyme preparation.					
35086703	12	38	theme	starch	1943:1948	arg1	concentration					1950:1962	dietary starch concentration	1935:1962	decreasing dietary starch concentration by 26%	1924:1969	Overall, decreasing dietary starch concentration by 26% decreased dry matter intake, milk, and ECM yields, but ECM feed efficiency was not different among treatments.					
35086703	12	39	theme	dietary	1935:1941	arg1	concentration					1950:1962	dietary starch concentration	1935:1962	decreasing dietary starch concentration by 26%	1924:1969	Overall, decreasing dietary starch concentration by 26% decreased dry matter intake, milk, and ECM yields, but ECM feed efficiency was not different among treatments.					
35086703	1	40	theme	lactating	343:351	arg1	cows					359:362	lactating dairy cows	343:362	lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control)	343:448	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	13	41	theme	dietary	2115:2121	arg1	starch					2123:2128	reducing dietary starch	2106:2128	reducing dietary starch	2106:2128	The negative effects of reducing dietary starch on production were not attenuated by the EEP.					
35086703	3	42	theme	%	802:802	arg1	starch					804:809	18.4% starch	798:809	18.4% starch	798:809	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	4	43	theme	feeding	1005:1011	arg1	time					997:1000	the time	993:1000	the time of feeding	993:1011	The EEP contained amylolytic and fibrolytic activities and was top-dressed on the total mixed ration at the time of feeding.					
35086703	0	44	theme	dairy	15:19	arg1	cows					21:24	dairy cows	15:24	dairy cows	15:24	Performance of dairy cows fed normal- or reduced-starch diets supplemented with an exogenous enzyme preparation.					
35086703	1	45	theme	nutrients	277:285	arg1	performance					235:245	performance	235:245	performance	235:245	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	45	theme	nutrients	277:285	arg1	digestibility					260:272	total-tract digestibility	248:272	total-tract digestibility of nutrients	248:285	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	45	theme	nutrients	277:285	arg1	profile					298:304	plasma AA profile	288:304	plasma AA profile	288:304	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	45	theme	nutrients	277:285	arg1	composition					328:338	milk fatty acids composition	311:338	milk fatty acids composition	311:338	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	13	46	theme	negative	2086:2093	arg1	effects					2095:2101	The negative effects	2082:2101	The negative effects of reducing dietary starch on production	2082:2142	The negative effects of reducing dietary starch on production were not attenuated by the EEP.					
35086703	11	47	theme	preformed	1884:1892	arg1	acids					1900:1904	preformed fatty acids	1884:1904	preformed fatty acids in milk	1884:1912	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	3	48	dep	diet	656:659	arg1	1					639:639	1	639:639	1	639:639	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	4	49	contain	contained	897:905	arg1	EEP					893:895	The EEP	889:895	The EEP	889:895	The EEP contained amylolytic and fibrolytic activities and was top-dressed on the total mixed ration at the time of feeding.					
35086703	4	49	contain	contained	897:905	arg2	activities					933:942	amylolytic and fibrolytic activities	907:942	amylolytic and fibrolytic activities	907:942	The EEP contained amylolytic and fibrolytic activities and was top-dressed on the total mixed ration at the time of feeding.					
35086703	5	50	dep	lower	1119:1123	arg1	average					1129:1135	average	1129:1135	average	1129:1135	Compared with normal-starch diet, dry matter intake and milk and energy-corrected milk (ECM) yields were lower (on average by 7.1, 9.5, and 7.2%, respectively) for cows on the RSD treatments.					
35086703	1	51	theme	plasma	288:293	arg1	profile					298:304	plasma AA profile	288:304	plasma AA profile	288:304	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	10	52	theme	Blood	1563:1567	arg1	concentration					1577:1589	Blood glucose concentration	1563:1589	Blood glucose concentration	1563:1589	Blood glucose concentration tended to be lower in cows on the RSD treatments, but ENZ increased glucose and tended to increase insulin concentrations at 4 h after feeding when compared with RSD.					
35086703	3	53	dep	RSD	833:835	arg1	3					830:830	3	830:830	3	830:830	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	1	54	theme	preparation	214:224	arg1	supplementation					175:189	supplementation	175:189	supplementation of an exogenous enzyme preparation (EEP)	175:230	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	3	55	theme	%	819:819	arg1	NDF					821:823	39.1% NDF	815:823	39.1% NDF	815:823	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	8	56	theme	NDF	1390:1392	arg1	digestibility					1373:1385	Total-tract digestibility	1361:1385	Total-tract digestibility of NDF	1361:1392	Total-tract digestibility of NDF tended to increase by RSD treatments.					
35086703	5	57	theme	normal-starch	1028:1040	arg1	diet					1042:1045	normal-starch diet	1028:1045	normal-starch diet	1028:1045	Compared with normal-starch diet, dry matter intake and milk and energy-corrected milk (ECM) yields were lower (on average by 7.1, 9.5, and 7.2%, respectively) for cows on the RSD treatments.					
35086703	3	58	theme	normal-starch	642:654	arg1	control					662:668	control	662:668	control	662:668	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	3	58	theme	normal-starch	642:654	arg1	diet					656:659	(1) normal-starch diet	638:659	(1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF)	638:754	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	1	59	from	profile	298:304	arg1	cows					359:362	lactating dairy cows	343:362	lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control)	343:448	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	2	60	theme	block	558:562	arg1	design					564:569	a 10-wk randomized complete block design	530:569	a 10-wk randomized complete block design experiment with 16 cows per treatment	530:607	Forty-eight Holstein cows (28 primiparous and 20 multiparous) were enrolled in a 10-wk randomized complete block design experiment with 16 cows per treatment.					
35086703	9	61	dep	increased	1526:1534	arg1	compared					1544:1551	compared	1544:1551	compared with RSD	1544:1560	Plasma AA concentrations were not affected by treatment, except that of 3-methylhistidine was increased by ENZ, compared with RSD.					
35086703	2	62	theme	randomized	538:547	arg1	block					558:562	a 10-wk randomized complete block	530:562	a 10-wk randomized complete block design experiment with 16 cows per treatment	530:607	Forty-eight Holstein cows (28 primiparous and 20 multiparous) were enrolled in a 10-wk randomized complete block design experiment with 16 cows per treatment.					
35086703	12	63	theme	feed	2030:2033	arg1	efficiency					2035:2044	ECM feed efficiency	2026:2044	ECM feed efficiency	2026:2044	Overall, decreasing dietary starch concentration by 26% decreased dry matter intake, milk, and ECM yields, but ECM feed efficiency was not different among treatments.					
35086703	1	64	theme	i.e.	426:429	arg1	control					441:447	i.e., positive control	426:447	control	441:447	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	64	theme	i.e.	426:429	arg1	diet					420:423	a normal-starch diet	404:423	a normal-starch diet (i.e., positive control)	404:448	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	6	65	theme	total	1254:1258	arg1	solids					1260:1265	total solids	1254:1265	total solids	1254:1265	Concentrations, but not yields, of milk fat and total solids were increased by RSD.					
35086703	1	66	from	digestibility	260:272	arg1	cows					359:362	lactating dairy cows	343:362	lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control)	343:448	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	67	theme	positive	432:439	arg1	control					441:447	i.e., positive control	426:447	control	441:447	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	67	theme	positive	432:439	arg1	diet					420:423	a normal-starch diet	404:423	a normal-starch diet (i.e., positive control)	404:448	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	10	68	from	cows	1613:1616	arg1	treatments					1629:1638	the RSD treatments	1621:1638	the RSD treatments	1621:1638	Blood glucose concentration tended to be lower in cows on the RSD treatments, but ENZ increased glucose and tended to increase insulin concentrations at 4 h after feeding when compared with RSD.					
35086703	11	69	theme	fatty	1825:1829	arg1	acids					1831:1835	de novo fatty acids	1817:1835	de novo fatty acids	1817:1835	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	3	70	theme	dry	685:687	arg1	basis					696:700	% dry matter basis	683:700	% dry matter basis	683:700	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	11	71	theme	de	1817:1818	arg1	acids					1831:1835	de novo fatty acids	1817:1835	de novo fatty acids	1817:1835	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	2	72	with	experiment	571:580	arg1	cows					590:593	16 cows	587:593	16 cows per treatment	587:607	Forty-eight Holstein cows (28 primiparous and 20 multiparous) were enrolled in a 10-wk randomized complete block design experiment with 16 cows per treatment.					
35086703	3	73	contain	containing	787:796	arg2	starch					804:809	18.4% starch	798:809	18.4% starch	798:809	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	3	73	contain	containing	787:796	arg1	RSD					782:784	RSD	782:784	RSD	782:784	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	3	73	contain	containing	787:796	arg1	diet					776:779	(2) reduced-starch diet	757:779	(2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF	757:823	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	3	73	contain	containing	787:796	arg2	NDF					821:823	39.1% NDF	815:823	39.1% NDF	815:823	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	12	74	theme	ECM	2010:2012	arg1	yields					2014:2019	ECM yields	2010:2019	ECM yields	2010:2019	Overall, decreasing dietary starch concentration by 26% decreased dry matter intake, milk, and ECM yields, but ECM feed efficiency was not different among treatments.					
35086703	13	75	from	effects	2095:2101	arg1	production					2133:2142	production	2133:2142	production	2133:2142	The negative effects of reducing dietary starch on production were not attenuated by the EEP.					
35086703	1	76	theme	exogenous	197:205	arg1	EEP					227:229	EEP	227:229	EEP	227:229	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	76	theme	exogenous	197:205	arg1	preparation					214:224	an exogenous enzyme preparation	194:224	an exogenous enzyme preparation (EEP)	194:230	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	9	77	theme	AA	1439:1440	arg1	concentrations					1442:1455	Plasma AA concentrations	1432:1455	Plasma AA concentrations	1432:1455	Plasma AA concentrations were not affected by treatment, except that of 3-methylhistidine was increased by ENZ, compared with RSD.					
35086703	3	78	theme	24.8	703:706	arg1	%					707:707	%	707:707	%	707:707	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	13	79	theme	starch	2123:2128	arg1	effects					2095:2101	The negative effects	2082:2101	The negative effects of reducing dietary starch on production	2082:2142	The negative effects of reducing dietary starch on production were not attenuated by the EEP.					
35086703	1	80	theme	reduced-starch	370:383	arg1	diet					385:388	a reduced-starch diet	368:388	a reduced-starch diet	368:388	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	7	81	theme	milk	1307:1310	arg1	efficiency					1317:1326	Energy-corrected milk feed efficiency	1290:1326	Energy-corrected milk feed efficiency	1290:1326	Energy-corrected milk feed efficiency did not differ among treatments.					
35086703	11	82	theme	RSD	1770:1772	arg1	treatments					1774:1783	the RSD treatments	1766:1783	the RSD treatments	1766:1783	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	3	83	theme	33.0	720:723	arg1	%					724:724	%	724:724	%	724:724	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	11	84	from	Cows	1758:1761	arg1	treatments					1774:1783	the RSD treatments	1766:1783	the RSD treatments	1766:1783	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	1	85	theme	study	135:139	arg1	objective					117:125	The objective	113:125	The objective of this study	113:139	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	2	86	theme	Holstein	463:470	arg1	cows					472:475	Forty-eight Holstein cows	451:475	Forty-eight Holstein cows (28 primiparous and 20 multiparous)	451:511	Forty-eight Holstein cows (28 primiparous and 20 multiparous) were enrolled in a 10-wk randomized complete block design experiment with 16 cows per treatment.					
35086703	4	87	theme	total	971:975	arg1	ration					983:988	the total mixed ration	967:988	the total mixed ration	967:988	The EEP contained amylolytic and fibrolytic activities and was top-dressed on the total mixed ration at the time of feeding.					
35086703	1	88	theme	milk	311:314	arg1	composition					328:338	milk fatty acids composition	311:338	milk fatty acids composition	311:338	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	3	89	theme	EEP	878:880	arg1	day					868:870	day	868:870	day of an EEP (ENZ)	868:886	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	1	90	from	effects	164:170	arg1	performance					235:245	performance	235:245	performance	235:245	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	90	from	effects	164:170	arg1	digestibility					260:272	total-tract digestibility	248:272	total-tract digestibility of nutrients	248:285	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	90	from	effects	164:170	arg1	profile					298:304	plasma AA profile	288:304	plasma AA profile	288:304	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	1	90	from	effects	164:170	arg1	composition					328:338	milk fatty acids composition	311:338	milk fatty acids composition	311:338	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	3	91	theme	reduced-starch	761:774	arg1	RSD					782:784	RSD	782:784	RSD	782:784	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	3	91	theme	reduced-starch	761:774	arg1	diet					776:779	(2) reduced-starch diet	757:779	(2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF	757:823	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	12	92	theme	matter	1985:1990	arg1	intake					1992:1997	dry matter intake	1981:1997	dry matter intake	1981:1997	Overall, decreasing dietary starch concentration by 26% decreased dry matter intake, milk, and ECM yields, but ECM feed efficiency was not different among treatments.					
35086703	5	93	theme	matter	1052:1057	arg1	intake					1059:1064	dry matter intake	1048:1064	dry matter intake	1048:1064	Compared with normal-starch diet, dry matter intake and milk and energy-corrected milk (ECM) yields were lower (on average by 7.1, 9.5, and 7.2%, respectively) for cows on the RSD treatments.					
35086703	0	94	theme	enzyme	93:98	arg1	preparation					100:110	an exogenous enzyme preparation	80:110	an exogenous enzyme preparation	80:110	Performance of dairy cows fed normal- or reduced-starch diets supplemented with an exogenous enzyme preparation.					
35086703	1	95	theme	acids	322:326	arg1	composition					328:338	milk fatty acids composition	311:338	milk fatty acids composition	311:338	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	3	96	theme	neutral	726:732	arg1	NDF					751:753	NDF	751:753	NDF	751:753	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	3	96	theme	neutral	726:732	arg1	fiber					744:748	33.0% neutral detergent fiber	720:748	33.0% neutral detergent fiber (NDF)	720:754	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	13	97	theme	reducing	2106:2113	arg1	starch					2123:2128	reducing dietary starch	2106:2128	reducing dietary starch	2106:2128	The negative effects of reducing dietary starch on production were not attenuated by the EEP.					
35086703	5	98	theme	RSD	1190:1192	arg1	treatments					1194:1203	the RSD treatments	1186:1203	the RSD treatments	1186:1203	Compared with normal-starch diet, dry matter intake and milk and energy-corrected milk (ECM) yields were lower (on average by 7.1, 9.5, and 7.2%, respectively) for cows on the RSD treatments.					
35086703	12	99	theme	decreasing	1924:1933	arg1	concentration					1950:1962	dietary starch concentration	1935:1962	decreasing dietary starch concentration by 26%	1924:1969	Overall, decreasing dietary starch concentration by 26% decreased dry matter intake, milk, and ECM yields, but ECM feed efficiency was not different among treatments.					
35086703	1	100	theme	dairy	353:357	arg1	cows					359:362	lactating dairy cows	343:362	lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control)	343:448	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	11	101	theme	increased	1856:1864	arg1	concentrations					1866:1879	increased concentrations	1856:1879	increased concentrations of preformed fatty acids in milk	1856:1912	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	3	102	contain	containing	671:680	arg2	fiber					744:748	33.0% neutral detergent fiber	720:748	33.0% neutral detergent fiber (NDF)	720:754	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	3	102	contain	containing	671:680	arg1	control					662:668	control	662:668	control	662:668	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	3	102	contain	containing	671:680	arg2	starch					709:714	(% dry matter basis) 24.8% starch	682:714	(% dry matter basis) 24.8% starch	682:714	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	3	102	contain	containing	671:680	arg1	diet					656:659	(1) normal-starch diet	638:659	(1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF)	638:754	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	3	102	contain	containing	671:680	arg2	NDF					751:753	NDF	751:753	NDF	751:753	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	0	103	theme	cows	21:24	arg1	Performance					0:10	Performance	0:10	Performance of dairy cows	0:24	Performance of dairy cows fed normal- or reduced-starch diets supplemented with an exogenous enzyme preparation.					
35086703	0	104	theme	normal-	30:36	arg1	diets					56:60	normal- or reduced-starch diets	30:60	normal- or reduced-starch diets supplemented with an exogenous enzyme preparation	30:110	Performance of dairy cows fed normal- or reduced-starch diets supplemented with an exogenous enzyme preparation.					
35086703	3	105	theme	18.4	798:801	arg1	%					802:802	%	802:802	%	802:802	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	11	106	theme	fatty	1894:1898	arg1	acids					1900:1904	preformed fatty acids	1884:1904	preformed fatty acids in milk	1884:1912	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	10	107	theme	glucose	1569:1575	arg1	concentration					1577:1589	Blood glucose concentration	1563:1589	Blood glucose concentration	1563:1589	Blood glucose concentration tended to be lower in cows on the RSD treatments, but ENZ increased glucose and tended to increase insulin concentrations at 4 h after feeding when compared with RSD.					
35086703	0	108	theme	reduced-starch	41:54	arg1	diets					56:60	normal- or reduced-starch diets	30:60	normal- or reduced-starch diets supplemented with an exogenous enzyme preparation	30:110	Performance of dairy cows fed normal- or reduced-starch diets supplemented with an exogenous enzyme preparation.					
35086703	1	109	theme	AA	295:296	arg1	profile					298:304	plasma AA profile	288:304	plasma AA profile	288:304	The objective of this study was to investigate the effects of supplementation of an exogenous enzyme preparation (EEP) on performance, total-tract digestibility of nutrients, plasma AA profile, and milk fatty acids composition in lactating dairy cows fed a reduced-starch diet compared with a normal-starch diet (i.e., positive control).					
35086703	11	110	from	concentrations	1866:1879	arg1	milk					1909:1912	milk	1909:1912	milk	1909:1912	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	11	111	from	acids	1900:1904	arg1	milk					1909:1912	milk	1909:1912	milk	1909:1912	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	2	112	dep	cows	472:475	arg1	primiparous					481:491	primiparous	481:491	primiparous	481:491	Forty-eight Holstein cows (28 primiparous and 20 multiparous) were enrolled in a 10-wk randomized complete block design experiment with 16 cows per treatment.					
35086703	2	112	dep	cows	472:475	arg1	multiparous					500:510	multiparous	500:510	multiparous	500:510	Forty-eight Holstein cows (28 primiparous and 20 multiparous) were enrolled in a 10-wk randomized complete block design experiment with 16 cows per treatment.					
35086703	3	113	theme	39.1	815:818	arg1	%					819:819	%	819:819	%	819:819	Treatments were as follows: (1) normal-starch diet (control) containing (% dry matter basis) 24.8% starch and 33.0% neutral detergent fiber (NDF), (2) reduced-starch diet (RSD) containing 18.4% starch and 39.1% NDF, or (3) RSD supplemented with 10 g/cow per day of an EEP (ENZ).					
35086703	11	114	from	milk	1909:1912	arg1	concentrations					1866:1879	increased concentrations	1856:1879	increased concentrations of preformed fatty acids in milk	1856:1912	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	5	115	from	cows	1178:1181	arg1	treatments					1194:1203	the RSD treatments	1186:1203	the RSD treatments	1186:1203	Compared with normal-starch diet, dry matter intake and milk and energy-corrected milk (ECM) yields were lower (on average by 7.1, 9.5, and 7.2%, respectively) for cows on the RSD treatments.					
35086703	10	116	theme	RSD	1625:1627	arg1	treatments					1629:1638	the RSD treatments	1621:1638	the RSD treatments	1621:1638	Blood glucose concentration tended to be lower in cows on the RSD treatments, but ENZ increased glucose and tended to increase insulin concentrations at 4 h after feeding when compared with RSD.					
35086703	11	117	contain	have	1851:1854	arg1	Cows					1758:1761	Cows	1758:1761	Cows on the RSD treatments	1758:1783	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	11	117	contain	have	1851:1854	arg2	concentrations					1866:1879	increased concentrations	1856:1879	increased concentrations of preformed fatty acids in milk	1856:1912	Cows on the RSD treatments had decreased concentrations of de novo fatty acids and tended to have increased concentrations of preformed fatty acids in milk.					
35086703	10	118	theme	insulin	1690:1696	arg1	concentrations					1698:1711	insulin concentrations	1690:1711	insulin concentrations	1690:1711	Blood glucose concentration tended to be lower in cows on the RSD treatments, but ENZ increased glucose and tended to increase insulin concentrations at 4 h after feeding when compared with RSD.					
36592751	5	0	dep	impact	1108:1113	arg1	G					1126:1126	G	1126:1126	impact (P = 0.74) G	1108:1126	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	8	1	theme	mill	1692:1695	arg1	type					1697:1700	mill type	1692:1700	mill type	1692:1700	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	2	2	theme	type	499:502	arg1	effect					465:470	the effect	461:470	the effect of milling method and corn type on performance and carcass characteristics	461:545	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	8	3	theme	≤	1582:1582	arg1	P					1580:1580	P ≤ 0.01	1580:1587	OM; P ≤ 0.01	1576:1587	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	2	4	from	effect	465:470	arg1	performance					507:517	performance	507:517	performance	507:517	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	2	4	from	effect	465:470	arg1	characteristics					531:545	carcass characteristics	523:545	carcass characteristics	523:545	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	3	5	theme	×	581:581	arg1	method					629:634	milling method	621:634	milling method (Automatic Ag roller mill [ROLL]	621:667	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	5	theme	×	581:581	arg1	design					595:600	a 2 × 3 factorial design	577:600	a 2 × 3 factorial design with factors	577:613	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	5	6	theme	fecal	1033:1037	arg1	starch					1039:1044	fecal starch	1033:1044	55% lower fecal starch (P < 0.01) compared to HAMMER HMC	1023:1078	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	5	6	theme	fecal	1033:1037	arg1	P					1047:1047	P < 0.01	1047:1054	P < 0.01	1047:1054	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	11	7	theme	milling	2219:2225	arg1	method					2227:2232	milling method	2219:2232	milling method	2219:2232	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	1	8	theme	dry	264:266	arg1	corn					268:271	dry corn	264:271	dry corn	264:271	Two experiments were conducted to evaluate the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion.					
36592751	13	9	theme	milling	2512:2518	arg1	method					2520:2525	milling method	2512:2525	milling method	2512:2525	Furthermore, feeding cattle HMC compared to DC increases nutrient digestibility, but milling method had little impact.					
36592751	2	10	theme	carcass	523:529	arg1	characteristics					531:545	carcass characteristics	523:545	carcass characteristics	523:545	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	5	11	dep	fed	962:964	arg1	had					975:977	had	975:977	had	975:977	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	5	11	dep	fed	962:964	arg1	F					1128:1128	F	1128:1128	F in DC diets	1128:1140	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	2	12	theme	initial	390:396	arg1	weight					403:408	initial body weight [BW]	390:413	initial body weight [BW]	390:413	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	7	13	dep	×	1440:1440	arg1	design					1464:1469	factorial treatment design	1444:1469	2 × 2 factorial treatment design	1438:1469	In experiment 2, seven ruminally fistulated steers were utilized in a 4 × 7 incomplete Latin rectangle to evaluate the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion.					
36592751	3	14	theme	Automatic	637:645	arg1	method					629:634	milling method	621:634	milling method (Automatic Ag roller mill [ROLL]	621:667	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	14	theme	Automatic	637:645	arg1	[ROLL					662:666	Automatic Ag roller mill [ROLL	637:666	Automatic Ag roller mill [ROLL	637:666	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	11	15	theme	greater	2145:2151	arg1	area					2153:2156	greater area	2145:2156	greater area less than pH 5.6 (P = 0.05)	2145:2184	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	11	15	theme	greater	2145:2151	arg1	P					2176:2176	P = 0.05	2176:2183	P = 0.05	2176:2183	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	9	16	theme	milling	1763:1769	arg1	treatments					1771:1780	milling treatments	1763:1780	milling treatments fed as HMC (P ≥ 0.69)	1763:1802	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	7	17	theme	factorial	1444:1452	arg1	design					1464:1469	factorial treatment design	1444:1469	2 × 2 factorial treatment design	1438:1469	In experiment 2, seven ruminally fistulated steers were utilized in a 4 × 7 incomplete Latin rectangle to evaluate the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion.					
36592751	9	18	theme	P	1794:1794	arg1	HMC					1789:1791	HMC	1789:1791	HMC (P ≥ 0.69)	1789:1802	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	9	18	theme	P	1794:1794	arg1	≥					1796:1796	P ≥ 0.69	1794:1801	P ≥ 0.69	1794:1801	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	4	19	theme	dry	862:864	arg1	intake					873:878	dry matter intake	862:878	dry matter intake	862:878	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	0	20	theme	carcass	105:111	arg1	characteristics					113:127	carcass characteristics	105:127	carcass characteristics	105:127	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.					
36592751	13	21	theme	cattle	2448:2453	arg1	HMC					2455:2457	feeding cattle HMC	2440:2457	feeding cattle HMC compared to DC	2440:2472	Furthermore, feeding cattle HMC compared to DC increases nutrient digestibility, but milling method had little impact.					
36592751	6	22	theme	carcass	1202:1208	arg1	traits					1210:1215	performance or carcass traits	1187:1215	traits	1210:1215	There were no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type.					
36592751	9	23	dep	digestible	1837:1846	arg1	P					1866:1866	P = 0.05	1866:1873	P = 0.05	1866:1873	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	9	23	dep	digestible	1837:1846	arg1	diet					1823:1826	the HAMMER DC diet	1809:1826	the HAMMER DC diet	1809:1826	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	9	23	dep	digestible	1837:1846	arg1	digestible					1837:1846	digestible	1837:1846	digestible	1837:1846	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	12	24	theme	hammer	2345:2350	arg1	mill					2352:2355	a hammer mill	2343:2355	a hammer mill	2343:2355	Processing HMC with a roller mill improved G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend.					
36592751	1	25	from	effect	201:206	arg1	digestion					335:343	nutrient digestion	326:343	nutrient digestion	326:343	Two experiments were conducted to evaluate the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion.					
36592751	1	25	from	effect	201:206	arg1	performance					293:303	finishing cattle performance	276:303	finishing cattle performance	276:303	Two experiments were conducted to evaluate the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion.					
36592751	1	25	from	effect	201:206	arg1	traits					314:319	carcass traits	306:319	carcass traits	306:319	Two experiments were conducted to evaluate the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion.					
36592751	2	26	theme	milling	475:481	arg1	method					483:488	milling method	475:488	milling method	475:488	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	6	27	theme	performance	1187:1197	arg1	traits					1210:1215	performance or carcass traits	1187:1215	traits	1210:1215	There were no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type.					
36592751	3	28	theme	roller	650:655	arg1	method					629:634	milling method	621:634	milling method (Automatic Ag roller mill [ROLL]	621:667	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	28	theme	roller	650:655	arg1	[ROLL					662:666	Automatic Ag roller mill [ROLL	637:666	Automatic Ag roller mill [ROLL	637:666	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	0	29	theme	nutrient	134:141	arg1	digestion					143:151	nutrient digestion	134:151	nutrient digestion	134:151	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.					
36592751	8	30	dep	OM	1576:1577	arg1	P					1580:1580	P ≤ 0.01	1580:1587	OM; P ≤ 0.01	1576:1587	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	8	31	theme	P	1641:1641	arg1	tendency					1631:1638	a tendency	1629:1638	a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility	1629:1728	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	8	31	theme	P	1641:1641	arg1	0.13					1645:1648	P ≤ 0.13	1641:1648	P ≤ 0.13	1641:1648	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	8	32	dep	matter	1568:1573	arg1	OM					1576:1577	OM	1576:1577	OM; P ≤ 0.01	1576:1587	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	4	33	dep	≥	888:888	arg1	DMI					881:883	DMI	881:883	DMI	881:883	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	13	34	theme	feeding	2440:2446	arg1	HMC					2455:2457	feeding cattle HMC	2440:2457	feeding cattle HMC compared to DC	2440:2472	Furthermore, feeding cattle HMC compared to DC increases nutrient digestibility, but milling method had little impact.					
36592751	5	35	dep	%	1025:1025	arg1	starch					1039:1044	fecal starch	1033:1044	55% lower fecal starch (P < 0.01) compared to HAMMER HMC	1023:1078	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	5	35	dep	%	1025:1025	arg1	P					1047:1047	P < 0.01	1047:1054	P < 0.01	1047:1054	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	2	36	theme	±	421:421	arg1	kg					426:427	402 ± 17 kg	417:427	402 ± 17 kg	417:427	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	8	37	dep	DM	1706:1707	arg1	digestibility					1716:1728	digestibility	1716:1728	digestibility	1716:1728	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	1	38	theme	nutrient	326:333	arg1	digestion					335:343	nutrient digestion	326:343	nutrient digestion	326:343	Two experiments were conducted to evaluate the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion.					
36592751	6	39	theme	P	1174:1174	arg1	effects					1165:1171	no further effects	1154:1171	no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type	1154:1257	There were no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type.					
36592751	6	39	theme	P	1174:1174	arg1	≥					1176:1176	P ≥ 0.14	1174:1181	P ≥ 0.14	1174:1181	There were no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type.					
36592751	7	40	from	effects	1379:1385	arg1	digestion					1484:1492	nutrient digestion	1475:1492	nutrient digestion	1475:1492	In experiment 2, seven ruminally fistulated steers were utilized in a 4 × 7 incomplete Latin rectangle to evaluate the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion.					
36592751	0	41	from	Impact	0:5	arg1	digestion					143:151	nutrient digestion	134:151	nutrient digestion	134:151	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.					
36592751	0	41	from	Impact	0:5	arg1	performance					92:102	finishing cattle performance	75:102	finishing cattle performance	75:102	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.					
36592751	0	41	from	Impact	0:5	arg1	characteristics					113:127	carcass characteristics	105:127	carcass characteristics	105:127	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.					
36592751	12	42	theme	DC	2417:2418	arg1	blend					2420:2424	DC blend	2417:2424	G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend	2311:2424	Processing HMC with a roller mill improved G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend.					
36592751	0	43	theme	finishing	75:83	arg1	performance					92:102	finishing cattle performance	75:102	finishing cattle performance	75:102	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.					
36592751	9	44	theme	DC	1820:1821	arg1	P					1866:1866	P = 0.05	1866:1873	P = 0.05	1866:1873	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	9	44	theme	DC	1820:1821	arg1	diet					1823:1826	the HAMMER DC diet	1809:1826	the HAMMER DC diet	1809:1826	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	9	44	theme	DC	1820:1821	arg1	digestible					1837:1846	digestible	1837:1846	digestible	1837:1846	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	6	45	theme	milling	1231:1237	arg1	method					1239:1244	milling method	1231:1244	milling method	1231:1244	There were no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type.					
36592751	5	46	theme	<	1049:1049	arg1	starch					1039:1044	fecal starch	1033:1044	55% lower fecal starch (P < 0.01) compared to HAMMER HMC	1023:1078	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	5	46	theme	<	1049:1049	arg1	P					1047:1047	P < 0.01	1047:1054	P < 0.01	1047:1054	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	11	47	theme	less	2158:2161	arg1	area					2153:2156	greater area	2145:2156	greater area less than pH 5.6 (P = 0.05)	2145:2184	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	11	47	theme	less	2158:2161	arg1	P					2176:2176	P = 0.05	2176:2183	P = 0.05	2176:2183	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	2	48	theme	body	398:401	arg1	weight					403:408	initial body weight [BW]	390:413	initial body weight [BW]	390:413	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	11	49	theme	ruminal	2083:2089	arg1	pH					2091:2092	average ruminal pH	2075:2092	average ruminal pH	2075:2092	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	11	50	dep	=	2133:2133	arg1	0.04					2135:2138	0.04	2135:2138	0.04	2135:2138	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	7	51	theme	HMC	1396:1398	arg1	effects					1379:1385	the effects	1375:1385	the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion	1375:1492	In experiment 2, seven ruminally fistulated steers were utilized in a 4 × 7 incomplete Latin rectangle to evaluate the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion.					
36592751	8	52	theme	excreted	1531:1538	arg1	DM					1552:1553	DM	1552:1553	DM	1552:1553	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	8	52	theme	excreted	1531:1538	arg1	matter					1544:1549	excreted dry matter	1531:1549	excreted dry matter (DM)	1531:1554	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	4	53	theme	final	837:841	arg1	BW					843:844	final BW	837:844	final BW	837:844	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	10	54	theme	HMC-based	1890:1898	arg1	diets					1900:1904	HMC-based diets	1890:1904	HMC-based diets	1890:1904	As expected, HMC-based diets had greater (P < 0.01) starch digestibility compared to DC, but milling method had no impact on starch digestibility (P = 0.56).					
36592751	0	55	theme	different	10:18	arg1	methods					33:39	different corn milling methods	10:39	different corn milling methods for high-moisture and dry corn	10:70	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.					
36592751	1	56	theme	finishing	276:284	arg1	performance					293:303	finishing cattle performance	276:303	finishing cattle performance	276:303	Two experiments were conducted to evaluate the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion.					
36592751	7	57	theme	DC	1390:1391	arg1	effects					1379:1385	the effects	1375:1385	the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion	1375:1492	In experiment 2, seven ruminally fistulated steers were utilized in a 4 × 7 incomplete Latin rectangle to evaluate the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion.					
36592751	12	58	dep	F	2313:2313	arg1	had					2362:2364	had	2362:2364	had little effect when corn was fed as dry corn or HMC	2362:2415	Processing HMC with a roller mill improved G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend.					
36592751	12	58	dep	F	2313:2313	arg1	compared					2315:2322	compared	2315:2322	compared to processing with a hammer mill	2315:2355	Processing HMC with a roller mill improved G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend.					
36592751	5	59	dep	G	1003:1003	arg1	F					1005:1005	F	1005:1005	G:F; P ≤ 0.01	1003:1015	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	5	59	dep	G	1003:1003	arg1	0.01					1012:1015	0.01	1012:1015	0.01	1012:1015	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	5	59	dep	G	1003:1003	arg1	P					1008:1008	P	1008:1008	G:F; P ≤ 0.01	1003:1015	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	8	60	theme	matter	1544:1549	arg1	matter					1568:1573	organic matter	1560:1573	organic matter (OM; P ≤ 0.01)	1560:1588	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	8	60	theme	matter	1544:1549	arg1	amount					1521:1526	the amount	1517:1526	the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01)	1517:1588	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	8	60	theme	matter	1544:1549	arg1	DM					1552:1553	DM	1552:1553	DM	1552:1553	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	8	60	theme	matter	1544:1549	arg1	matter					1544:1549	excreted dry matter	1531:1549	excreted dry matter (DM)	1531:1554	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	4	61	theme	method	801:806	arg1	interactions					820:831	no milling method × corn type interactions	790:831	no milling method × corn type interactions	790:831	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	4	61	theme	method	801:806	arg1	interaction					923:933	an interaction	920:933	an interaction for G:F (P = 0.09)	920:952	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	5	62	theme	greater	984:990	arg1	gain					992:995	4.7% greater gain	979:995	4.7% greater gain:feed (G:F; P ≤ 0.01)	979:1016	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	0	63	theme	milling	25:31	arg1	methods					33:39	different corn milling methods	10:39	different corn milling methods for high-moisture and dry corn	10:70	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.					
36592751	8	64	theme	Feeding	1495:1501	arg1	HMC					1503:1505	Feeding HMC	1495:1505	Feeding HMC	1495:1505	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	12	65	dep	G	2311:2311	arg1	F					2313:2313	F	2313:2313	G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend	2311:2424	Processing HMC with a roller mill improved G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend.					
36592751	12	65	dep	G	2311:2311	arg1	blend					2420:2424	DC blend	2417:2424	G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend	2311:2424	Processing HMC with a roller mill improved G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend.					
36592751	10	66	contain	had	1906:1908	arg2	digestibility					1936:1948	greater (P < 0.01) starch digestibility	1910:1948	greater (P < 0.01) starch digestibility	1910:1948	As expected, HMC-based diets had greater (P < 0.01) starch digestibility compared to DC, but milling method had no impact on starch digestibility (P = 0.56).					
36592751	10	66	contain	had	1906:1908	arg1	diets					1900:1904	HMC-based diets	1890:1904	HMC-based diets	1890:1904	As expected, HMC-based diets had greater (P < 0.01) starch digestibility compared to DC, but milling method had no impact on starch digestibility (P = 0.56).					
36592751	4	67	theme	corn	810:813	arg1	interactions					820:831	no milling method × corn type interactions	790:831	no milling method × corn type interactions	790:831	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	4	67	theme	corn	810:813	arg1	interaction					923:933	an interaction	920:933	an interaction for G:F (P = 0.09)	920:952	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	12	68	theme	roller	2290:2295	arg1	mill					2297:2300	a roller mill	2288:2300	a roller mill	2288:2300	Processing HMC with a roller mill improved G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend.					
36592751	1	69	theme	carcass	306:312	arg1	traits					314:319	carcass traits	306:319	carcass traits	306:319	Two experiments were conducted to evaluate the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion.					
36592751	8	70	theme	corn	1678:1681	arg1	type					1683:1686	corn type	1678:1686	corn type	1678:1686	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	13	71	contain	had	2527:2529	arg2	impact					2538:2543	little impact	2531:2543	little impact	2531:2543	Furthermore, feeding cattle HMC compared to DC increases nutrient digestibility, but milling method had little impact.					
36592751	13	71	contain	had	2527:2529	arg1	method					2520:2525	milling method	2512:2525	milling method	2512:2525	Furthermore, feeding cattle HMC compared to DC increases nutrient digestibility, but milling method had little impact.					
36592751	5	72	dep	gain	992:995	arg1	G					1003:1003	G	1003:1003	G:F; P ≤ 0.01	1003:1015	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	5	72	dep	gain	992:995	arg1	feed					997:1000	feed	997:1000	4.7% greater gain:feed (G:F; P ≤ 0.01)	979:1016	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	11	73	dep	=	2064:2064	arg1	0.56					2066:2069	0.56	2066:2069	0.56	2066:2069	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	4	74	theme	=	946:946	arg1	F					941:941	F	941:941	G:F (P = 0.09)	939:952	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	4	74	theme	=	946:946	arg1	P					944:944	P = 0.09	944:951	P = 0.09	944:951	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	2	75	dep	steers	363:368	arg1	=					415:415	=	415:415	=	415:415	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	10	76	theme	P	1919:1919	arg1	<					1921:1921	P < 0.01	1919:1926	P < 0.01	1919:1926	As expected, HMC-based diets had greater (P < 0.01) starch digestibility compared to DC, but milling method had no impact on starch digestibility (P = 0.56).					
36592751	1	77	theme	different	211:219	arg1	methods					234:240	different corn milling methods	211:240	different corn milling methods for high-moisture and dry corn	211:271	Two experiments were conducted to evaluate the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion.					
36592751	11	78	theme	greater	2113:2119	arg1	variance					2121:2128	greater variance	2113:2128	greater variance (P = 0.04)	2113:2139	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	5	79	from	F	1128:1128	arg1	diets					1136:1140	DC diets	1133:1140	DC diets	1133:1140	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	8	80	theme	matter	1568:1573	arg1	matter					1568:1573	organic matter	1560:1573	organic matter (OM; P ≤ 0.01)	1560:1588	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	8	80	theme	matter	1568:1573	arg1	amount					1521:1526	the amount	1517:1526	the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01)	1517:1588	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	8	80	theme	matter	1568:1573	arg1	DM					1552:1553	DM	1552:1553	DM	1552:1553	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	8	80	theme	matter	1568:1573	arg1	matter					1544:1549	excreted dry matter	1531:1549	excreted dry matter (DM)	1531:1554	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	12	81	theme	Processing	2268:2277	arg1	HMC					2279:2281	Processing HMC	2268:2281	Processing HMC with a roller mill	2268:2300	Processing HMC with a roller mill improved G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend.					
36592751	1	82	theme	milling	226:232	arg1	methods					234:240	different corn milling methods	211:240	different corn milling methods for high-moisture and dry corn	211:271	Two experiments were conducted to evaluate the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion.					
36592751	11	83	theme	based	2201:2205	arg1	diets					2207:2211	DC based diets	2198:2211	DC based diets	2198:2211	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	5	84	theme	DC	1133:1134	arg1	diets					1136:1140	DC diets	1133:1140	DC diets	1133:1140	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	3	85	dep	evaluated	564:572	arg1	mill					679:682	mill	679:682	mill	679:682	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	85	dep	evaluated	564:572	arg1	of					755:756	of	755:756	of	755:756	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	85	dep	evaluated	564:572	arg1	type					703:706	type	703:706	type	703:706	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	85	dep	evaluated	564:572	arg1	[HAMMER					684:690	[HAMMER	684:690	[HAMMER	684:690	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	85	dep	evaluated	564:572	arg1	HMC					758:760	HMC	758:760	HMC	758:760	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	85	dep	evaluated	564:572	arg1	[HMC					723:726	[HMC	723:726	[HMC	723:726	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	85	dep	evaluated	564:572	arg1	[BLEND					769:774	[BLEND	769:774	[BLEND	769:774	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	85	dep	evaluated	564:572	arg1	dry					730:732	dry	730:732	dry	730:732	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	85	dep	evaluated	564:572	arg1	blend					749:753	blend	749:753	blend	749:753	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	85	dep	evaluated	564:572	arg1	[DC					734:736	[DC	734:736	[DC	734:736	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	2	86	theme	corn	494:497	arg1	type					499:502	corn type	494:502	corn type	494:502	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	7	87	theme	fistulated	1293:1302	arg1	steers					1304:1309	seven ruminally fistulated steers	1277:1309	seven ruminally fistulated steers	1277:1309	In experiment 2, seven ruminally fistulated steers were utilized in a 4 × 7 incomplete Latin rectangle to evaluate the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion.					
36592751	3	88	with	design	595:600	arg1	factors					607:613	factors	607:613	factors	607:613	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	2	89	theme	method	483:488	arg1	effect					465:470	the effect	461:470	the effect of milling method and corn type on performance and carcass characteristics	461:545	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	3	90	theme	milling	621:627	arg1	method					629:634	milling method	621:634	milling method (Automatic Ag roller mill [ROLL]	621:667	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	90	theme	milling	621:627	arg1	design					595:600	a 2 × 3 factorial design	577:600	a 2 × 3 factorial design with factors	577:613	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	90	theme	milling	621:627	arg1	[ROLL					662:666	Automatic Ag roller mill [ROLL	637:666	Automatic Ag roller mill [ROLL	637:666	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	6	91	from	effects	1165:1171	arg1	type					1254:1257	corn type	1249:1257	corn type	1249:1257	There were no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type.					
36592751	6	91	from	effects	1165:1171	arg1	traits					1210:1215	performance or carcass traits	1187:1215	traits	1210:1215	There were no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type.					
36592751	7	92	theme	nutrient	1475:1482	arg1	digestion					1484:1492	nutrient digestion	1475:1492	nutrient digestion	1475:1492	In experiment 2, seven ruminally fistulated steers were utilized in a 4 × 7 incomplete Latin rectangle to evaluate the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion.					
36592751	8	93	theme	mill	1604:1607	arg1	type					1609:1612	mill type	1604:1612	mill type	1604:1612	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	4	94	theme	matter	866:871	arg1	intake					873:878	dry matter intake	862:878	dry matter intake	862:878	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	3	95	theme	Ag	647:648	arg1	method					629:634	milling method	621:634	milling method (Automatic Ag roller mill [ROLL]	621:667	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	95	theme	Ag	647:648	arg1	[ROLL					662:666	Automatic Ag roller mill [ROLL	637:666	Automatic Ag roller mill [ROLL	637:666	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	7	96	theme	treatment	1454:1462	arg1	design					1464:1469	factorial treatment design	1444:1469	2 × 2 factorial treatment design	1438:1469	In experiment 2, seven ruminally fistulated steers were utilized in a 4 × 7 incomplete Latin rectangle to evaluate the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion.					
36592751	12	97	theme	little	2366:2371	arg1	effect					2373:2378	little effect	2366:2378	little effect	2366:2378	Processing HMC with a roller mill improved G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend.					
36592751	13	98	theme	nutrient	2484:2491	arg1	digestibility					2493:2505	nutrient digestibility	2484:2505	nutrient digestibility	2484:2505	Furthermore, feeding cattle HMC compared to DC increases nutrient digestibility, but milling method had little impact.					
36592751	12	99	with	HMC	2279:2281	arg1	mill					2297:2300	a roller mill	2288:2300	a roller mill	2288:2300	Processing HMC with a roller mill improved G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend.					
36592751	9	100	theme	=	1868:1868	arg1	P					1866:1866	P = 0.05	1866:1873	P = 0.05	1866:1873	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	9	100	theme	=	1868:1868	arg1	diet					1823:1826	the HAMMER DC diet	1809:1826	the HAMMER DC diet	1809:1826	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	9	100	theme	=	1868:1868	arg1	digestible					1837:1846	digestible	1837:1846	digestible	1837:1846	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	11	101	theme	=	2178:2178	arg1	area					2153:2156	greater area	2145:2156	greater area less than pH 5.6 (P = 0.05)	2145:2184	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	11	101	theme	=	2178:2178	arg1	P					2176:2176	P = 0.05	2176:2183	P = 0.05	2176:2183	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	9	102	theme	HAMMER	1813:1818	arg1	P					1866:1866	P = 0.05	1866:1873	P = 0.05	1866:1873	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	9	102	theme	HAMMER	1813:1818	arg1	diet					1823:1826	the HAMMER DC diet	1809:1826	the HAMMER DC diet	1809:1826	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	9	102	theme	HAMMER	1813:1818	arg1	digestible					1837:1846	digestible	1837:1846	digestible	1837:1846	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	3	103	theme	mill	657:660	arg1	method					629:634	milling method	621:634	milling method (Automatic Ag roller mill [ROLL]	621:667	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	103	theme	mill	657:660	arg1	[ROLL					662:666	Automatic Ag roller mill [ROLL	637:666	Automatic Ag roller mill [ROLL	637:666	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	10	104	theme	milling	1970:1976	arg1	method					1978:1983	milling method	1970:1983	milling method	1970:1983	As expected, HMC-based diets had greater (P < 0.01) starch digestibility compared to DC, but milling method had no impact on starch digestibility (P = 0.56).					
36592751	5	105	theme	ROLL	966:969	arg1	HMC					971:973	ROLL HMC	966:973	ROLL HMC	966:973	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	0	106	theme	dry	63:65	arg1	corn					67:70	dry corn	63:70	dry corn	63:70	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.					
36592751	10	107	theme	starch	2002:2007	arg1	digestibility					2009:2021	starch digestibility	2002:2021	starch digestibility	2002:2021	As expected, HMC-based diets had greater (P < 0.01) starch digestibility compared to DC, but milling method had no impact on starch digestibility (P = 0.56).					
36592751	8	108	theme	≤	1643:1643	arg1	tendency					1631:1638	a tendency	1629:1638	a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility	1629:1728	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	8	108	theme	≤	1643:1643	arg1	0.13					1645:1648	P ≤ 0.13	1641:1648	P ≤ 0.13	1641:1648	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	6	109	theme	corn	1249:1252	arg1	type					1254:1257	corn type	1249:1257	corn type	1249:1257	There were no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type.					
36592751	5	110	theme	HAMMER	1069:1074	arg1	HMC					1076:1078	HAMMER HMC	1069:1078	HAMMER HMC	1069:1078	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	11	111	theme	=	2133:2133	arg1	P					2131:2131	P	2131:2131	P = 0.04	2131:2138	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	0	112	theme	cattle	85:90	arg1	performance					92:102	finishing cattle performance	75:102	finishing cattle performance	75:102	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.					
36592751	2	113	dep	=	415:415	arg1	N					371:371	N	371:371	N = 600 [60 pens]	371:387	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	10	114	contain	had	1985:1987	arg1	method					1978:1983	milling method	1970:1983	milling method	1970:1983	As expected, HMC-based diets had greater (P < 0.01) starch digestibility compared to DC, but milling method had no impact on starch digestibility (P = 0.56).					
36592751	10	114	contain	had	1985:1987	arg2	impact					1992:1997	no impact	1989:1997	no impact	1989:1997	As expected, HMC-based diets had greater (P < 0.01) starch digestibility compared to DC, but milling method had no impact on starch digestibility (P = 0.56).					
36592751	12	115	theme	dry	2401:2403	arg1	corn					2405:2408	dry corn	2401:2408	dry corn	2401:2408	Processing HMC with a roller mill improved G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend.					
36592751	12	115	theme	dry	2401:2403	arg1	corn					2385:2388	corn	2385:2388	corn	2385:2388	Processing HMC with a roller mill improved G:F compared to processing with a hammer mill, but had little effect when corn was fed as dry corn or HMC:DC blend.					
36592751	11	116	dep	variance	2121:2128	arg1	P					2131:2131	P	2131:2131	P = 0.04	2131:2138	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	6	117	theme	further	1157:1163	arg1	effects					1165:1171	no further effects	1154:1171	no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type	1154:1257	There were no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type.					
36592751	6	117	theme	further	1157:1163	arg1	≥					1176:1176	P ≥ 0.14	1174:1181	P ≥ 0.14	1174:1181	There were no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type.					
36592751	11	118	theme	=	2064:2064	arg1	P					2062:2062	P	2062:2062	P = 0.56	2062:2069	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	8	119	theme	dry	1540:1542	arg1	DM					1552:1553	DM	1552:1553	DM	1552:1553	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	8	119	theme	dry	1540:1542	arg1	matter					1544:1549	excreted dry matter	1531:1549	excreted dry matter (DM)	1531:1554	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	4	120	theme	type	815:818	arg1	interactions					820:831	no milling method × corn type interactions	790:831	no milling method × corn type interactions	790:831	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	4	120	theme	type	815:818	arg1	interaction					923:933	an interaction	920:933	an interaction for G:F (P = 0.09)	920:952	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	0	121	theme	corn	20:23	arg1	methods					33:39	different corn milling methods	10:39	different corn milling methods for high-moisture and dry corn	10:70	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.					
36592751	11	122	theme	HMC	2099:2101	arg1	diets					2103:2107	HMC diets	2099:2107	HMC diets	2099:2107	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	11	123	theme	average	2075:2081	arg1	pH					2091:2092	average ruminal pH	2075:2092	average ruminal pH	2075:2092	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	10	124	dep	greater	1910:1916	arg1	<					1921:1921	P < 0.01	1919:1926	P < 0.01	1919:1926	As expected, HMC-based diets had greater (P < 0.01) starch digestibility compared to DC, but milling method had no impact on starch digestibility (P = 0.56).					
36592751	0	125	theme	methods	33:39	arg1	Impact					0:5	Impact	0:5	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.	0:152	Impact of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass characteristics, and nutrient digestion.					
36592751	1	126	theme	cattle	286:291	arg1	performance					293:303	finishing cattle performance	276:303	finishing cattle performance	276:303	Two experiments were conducted to evaluate the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion.					
36592751	9	127	theme	ROLL	1857:1860	arg1	DC					1862:1863	the ROLL DC	1853:1863	the ROLL DC	1853:1863	There was no difference between milling treatments fed as HMC (P ≥ 0.69), but the HAMMER DC diet was more digestible than the ROLL DC (P = 0.05).					
36592751	11	128	theme	P	2257:2257	arg1	either					2249:2254	either	2249:2254	either	2249:2254	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	11	128	theme	P	2257:2257	arg1	>					2259:2259	P > 0.33	2257:2264	P > 0.33	2257:2264	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	11	129	dep	differences	2049:2059	arg1	P					2062:2062	P	2062:2062	P = 0.56	2062:2069	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	4	130	theme	milling	793:799	arg1	interactions					820:831	no milling method × corn type interactions	790:831	no milling method × corn type interactions	790:831	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	4	130	theme	milling	793:799	arg1	interaction					923:933	an interaction	920:933	an interaction for G:F (P = 0.09)	920:952	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	5	131	theme	=	1118:1118	arg1	P					1116:1116	P = 0.74	1116:1123	P = 0.74	1116:1123	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	5	131	theme	=	1118:1118	arg1	impact					1108:1113	impact	1108:1113	impact (P = 0.74) G	1108:1126	Cattle fed ROLL HMC had 4.7% greater gain:feed (G:F; P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to HAMMER HMC, whereas processing did not impact (P = 0.74) G:F in DC diets.					
36592751	2	132	dep	=	373:373	arg1	pens					383:386	600 [60 pens]	375:387	600 [60 pens]	375:387	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	2	133	theme	=	373:373	arg1	N					371:371	N	371:371	N = 600 [60 pens]	371:387	In experiment 1, steers (N = 600 [60 pens]; initial body weight [BW] = 402 ± 17 kg) were fed for 134 d to evaluate the effect of milling method and corn type on performance and carcass characteristics.					
36592751	13	134	theme	little	2531:2536	arg1	impact					2538:2543	little impact	2531:2543	little impact	2531:2543	Furthermore, feeding cattle HMC compared to DC increases nutrient digestibility, but milling method had little impact.					
36592751	7	135	theme	incomplete	1336:1345	arg1	rectangle					1353:1361	a 4 × 7 incomplete Latin rectangle	1328:1361	a 4 × 7 incomplete Latin rectangle	1328:1361	In experiment 2, seven ruminally fistulated steers were utilized in a 4 × 7 incomplete Latin rectangle to evaluate the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion.					
36592751	8	136	theme	organic	1560:1566	arg1	matter					1568:1573	organic matter	1560:1573	organic matter (OM; P ≤ 0.01)	1560:1588	Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility.					
36592751	4	137	theme	×	808:808	arg1	interactions					820:831	no milling method × corn type interactions	790:831	no milling method × corn type interactions	790:831	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	4	137	theme	×	808:808	arg1	interaction					923:933	an interaction	920:933	an interaction for G:F (P = 0.09)	920:952	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	10	138	theme	greater	1910:1916	arg1	digestibility					1936:1948	greater (P < 0.01) starch digestibility	1910:1948	greater (P < 0.01) starch digestibility	1910:1948	As expected, HMC-based diets had greater (P < 0.01) starch digestibility compared to DC, but milling method had no impact on starch digestibility (P = 0.56).					
36592751	11	139	contain	had	2109:2111	arg2	area					2153:2156	greater area	2145:2156	greater area less than pH 5.6 (P = 0.05)	2145:2184	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	11	139	contain	had	2109:2111	arg2	variance					2121:2128	greater variance	2113:2128	greater variance (P = 0.04)	2113:2139	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	11	139	contain	had	2109:2111	arg1	diets					2103:2107	HMC diets	2099:2107	HMC diets	2099:2107	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	11	139	contain	had	2109:2111	arg2	P					2176:2176	P = 0.05	2176:2183	P = 0.05	2176:2183	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	4	140	dep	G	939:939	arg1	F					941:941	F	941:941	G:F (P = 0.09)	939:952	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	4	140	dep	G	939:939	arg1	P					944:944	P = 0.09	944:951	P = 0.09	944:951	There were no milling method × corn type interactions for final BW, gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there tended to be an interaction for G:F (P = 0.09).					
36592751	7	141	theme	Latin	1347:1351	arg1	rectangle					1353:1361	a 4 × 7 incomplete Latin rectangle	1328:1361	a 4 × 7 incomplete Latin rectangle	1328:1361	In experiment 2, seven ruminally fistulated steers were utilized in a 4 × 7 incomplete Latin rectangle to evaluate the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion.					
36592751	7	142	theme	×	1332:1332	arg1	rectangle					1353:1361	a 4 × 7 incomplete Latin rectangle	1328:1361	a 4 × 7 incomplete Latin rectangle	1328:1361	In experiment 2, seven ruminally fistulated steers were utilized in a 4 × 7 incomplete Latin rectangle to evaluate the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion.					
36592751	10	143	theme	starch	1929:1934	arg1	digestibility					1936:1948	greater (P < 0.01) starch digestibility	1910:1948	greater (P < 0.01) starch digestibility	1910:1948	As expected, HMC-based diets had greater (P < 0.01) starch digestibility compared to DC, but milling method had no impact on starch digestibility (P = 0.56).					
36592751	1	144	theme	corn	221:224	arg1	methods					234:240	different corn milling methods	211:240	different corn milling methods for high-moisture and dry corn	211:271	Two experiments were conducted to evaluate the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion.					
36592751	11	145	theme	DC	2198:2199	arg1	diets					2207:2211	DC based diets	2198:2211	DC based diets	2198:2211	There were no differences (P = 0.56) in average ruminal pH, but HMC diets had greater variance (P = 0.04) and greater area less than pH 5.6 (P = 0.05) compared to DC based diets while milling method did not impact either (P > 0.33).					
36592751	3	146	theme	factorial	585:593	arg1	method					629:634	milling method	621:634	milling method (Automatic Ag roller mill [ROLL]	621:667	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	3	146	theme	factorial	585:593	arg1	design					595:600	a 2 × 3 factorial design	577:600	a 2 × 3 factorial design with factors	577:613	Treatments were evaluated as a 2 × 3 factorial design with factors being milling method (Automatic Ag roller mill [ROLL] or hammer mill [HAMMER]) and corn type (high-moisture [HMC], dry [DC], or 50:50 blend of HMC and DC [BLEND]).					
36592751	7	147	used	utilized	1316:1323	arg2	steers					1304:1309	seven ruminally fistulated steers	1277:1309	seven ruminally fistulated steers	1277:1309	In experiment 2, seven ruminally fistulated steers were utilized in a 4 × 7 incomplete Latin rectangle to evaluate the effects of DC or HMC processed with either ROLL or HAMMER (2 × 2 factorial treatment design) on nutrient digestion.					
36592751	1	148	theme	methods	234:240	arg1	effect					201:206	the effect	197:206	the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion	197:343	Two experiments were conducted to evaluate the effect of different corn milling methods for high-moisture and dry corn on finishing cattle performance, carcass traits, and nutrient digestion.					
36270678	7	0	from	perturbations	1013:1025	arg1	homeostasis					1034:1044	gut homeostasis	1030:1044	gut homeostasis	1030:1044	Several factors may disrupt the GIT microbiome's homeostasis leading to dysbiosis, characterized by an imbalanced equilibrium and perturbations in gut homeostasis.					
36270678	1	1	theme	bacteria	138:145	arg1	collection					124:133	a dynamic and diverse collection	102:133	a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome	102:192	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	5	2	from	alterations	636:646	arg1	function					671:678	function	671:678	function	671:678	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	5	2	from	alterations	636:646	arg1	composition					655:665	composition	655:665	composition	655:665	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	9	3	theme	Recent	1188:1193	arg1	evidence					1195:1202	Recent evidence	1188:1202	Recent evidence	1188:1202	Recent evidence suggests that utilizing medications, prebiotics, probiotics, and fecal microbiota transplantation (FMT) to manipulate the microbiome could be a viable method for treating NAFLD.					
36270678	4	4	theme	critical	459:466	arg1	role					468:471	a critical role	457:471	a critical role	457:471	These microorganisms play a critical role in the host's energy metabolism and homeostasis.					
36270678	5	5	theme	new	535:537	arg1	research					539:546	new research	535:546	new research	535:546	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	7	6	theme	gut	1030:1032	arg1	homeostasis					1034:1044	gut homeostasis	1030:1044	gut homeostasis	1030:1044	Several factors may disrupt the GIT microbiome's homeostasis leading to dysbiosis, characterized by an imbalanced equilibrium and perturbations in gut homeostasis.					
36270678	7	7	theme	Several	883:889	arg1	factors					891:897	Several factors	883:897	Several factors	883:897	Several factors may disrupt the GIT microbiome's homeostasis leading to dysbiosis, characterized by an imbalanced equilibrium and perturbations in gut homeostasis.					
36270678	2	8	contain	has	215:217	arg1	microbiome					204:213	The gut microbiome	196:213	The gut microbiome	196:213	The gut microbiome has a major impact on the host during homeostasis and disease.					
36270678	2	8	contain	has	215:217	arg2	impact					227:232	a major impact	219:232	a major impact on the host during homeostasis and disease	219:275	The gut microbiome has a major impact on the host during homeostasis and disease.					
36270678	6	9	theme	ecology	815:821	arg1	robust					861:866	robust	861:866	robust	861:866	The resilience of the GIT microbial ecology and its tolerance to perturbation are robust but not ideal.					
36270678	6	9	theme	ecology	815:821	arg1	tolerance					831:839	its tolerance	827:839	its tolerance to perturbation	827:855	The resilience of the GIT microbial ecology and its tolerance to perturbation are robust but not ideal.					
36270678	6	9	theme	ecology	815:821	arg1	resilience					783:792	The resilience	779:792	The resilience of the GIT microbial ecology	779:821	The resilience of the GIT microbial ecology and its tolerance to perturbation are robust but not ideal.					
36270678	5	10	theme	substantial	599:609	arg1	role					611:614	a substantial role	597:614	a substantial role	597:614	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	1	11	theme	human	58:62	arg1	GIT					88:90	GIT	88:90	GIT	88:90	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	1	11	theme	human	58:62	arg1	tract					81:85	The human gastrointestinal tract	54:85	The human gastrointestinal tract (GIT)	54:91	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	3	12	theme	morbidities	418:428	arg1	multitude					405:413	a multitude	403:413	a multitude of morbidities	403:428	The connection between both the host and the microbiome is complex, although its manipulation may assist prevent or treating a multitude of morbidities.					
36270678	6	13	theme	microbial	805:813	arg1	ecology					815:821	the GIT microbial ecology	797:821	the GIT microbial ecology	797:821	The resilience of the GIT microbial ecology and its tolerance to perturbation are robust but not ideal.					
36270678	1	14	theme	gastrointestinal	64:79	arg1	GIT					88:90	GIT	88:90	GIT	88:90	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	1	14	theme	gastrointestinal	64:79	arg1	tract					81:85	The human gastrointestinal tract	54:85	The human gastrointestinal tract (GIT)	54:91	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	1	15	theme	archaea	148:154	arg1	collection					124:133	a dynamic and diverse collection	102:133	a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome	102:192	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	6	16	theme	GIT	801:803	arg1	ecology					815:821	the GIT microbial ecology	797:821	the GIT microbial ecology	797:821	The resilience of the GIT microbial ecology and its tolerance to perturbation are robust but not ideal.					
36270678	2	17	theme	major	221:225	arg1	impact					227:232	a major impact	219:232	a major impact on the host during homeostasis and disease	219:275	The gut microbiome has a major impact on the host during homeostasis and disease.					
36270678	8	18	theme	metabolic	1096:1104	arg1	disorders					1106:1114	metabolic disorders	1096:1114	metabolic disorders	1096:1114	Irritable bowel disease (IBD), malnutrition, and metabolic disorders, such as NAFLD, have been associated with the dysbiotic gut microbiome.					
36270678	8	18	theme	metabolic	1096:1104	arg1	NAFLD					1125:1129	NAFLD	1125:1129	NAFLD	1125:1129	Irritable bowel disease (IBD), malnutrition, and metabolic disorders, such as NAFLD, have been associated with the dysbiotic gut microbiome.					
36270678	7	19	from	equilibrium	997:1007	arg1	homeostasis					1034:1044	gut homeostasis	1030:1044	gut homeostasis	1030:1044	Several factors may disrupt the GIT microbiome's homeostasis leading to dysbiosis, characterized by an imbalanced equilibrium and perturbations in gut homeostasis.					
36270678	9	20	theme	viable	1348:1353	arg1	method					1355:1360	a viable method	1346:1360	a viable method for treating NAFLD	1346:1379	Recent evidence suggests that utilizing medications, prebiotics, probiotics, and fecal microbiota transplantation (FMT) to manipulate the microbiome could be a viable method for treating NAFLD.					
36270678	0	21	theme	Gut	0:2	arg1	microbiome					4:13	Gut microbiome	0:13	Gut microbiome	0:13	Gut microbiome and non-alcoholic fatty liver disease.					
36270678	4	22	theme	energy	487:492	arg1	metabolism					494:503	the host's energy metabolism	476:503	the host's energy metabolism	476:503	These microorganisms play a critical role in the host's energy metabolism and homeostasis.					
36270678	5	23	theme	gastrointestinal	569:584	arg1	tract					586:590	the gastrointestinal tract	565:590	the gastrointestinal tract	565:590	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	1	24	theme	fungi	161:165	arg1	collection					124:133	a dynamic and diverse collection	102:133	a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome	102:192	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	0	25	theme	non-alcoholic	19:31	arg1	disease					45:51	non-alcoholic fatty liver disease	19:51	non-alcoholic fatty liver disease	19:51	Gut microbiome and non-alcoholic fatty liver disease.					
36270678	5	26	theme	non-alcoholic	736:748	arg1	NAFLD					771:775	NAFLD	771:775	NAFLD	771:775	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	5	26	theme	non-alcoholic	736:748	arg1	disease					762:768	non-alcoholic fatty liver disease	736:768	non-alcoholic fatty liver disease (NAFLD)	736:776	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	8	27	theme	dysbiotic	1162:1170	arg1	microbiome					1176:1185	the dysbiotic gut microbiome	1158:1185	the dysbiotic gut microbiome	1158:1185	Irritable bowel disease (IBD), malnutrition, and metabolic disorders, such as NAFLD, have been associated with the dysbiotic gut microbiome.					
36270678	5	28	theme	liver	756:760	arg1	NAFLD					771:775	NAFLD	771:775	NAFLD	771:775	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	5	28	theme	liver	756:760	arg1	disease					762:768	non-alcoholic fatty liver disease	736:768	non-alcoholic fatty liver disease (NAFLD)	736:776	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	5	29	from	microbes	553:560	arg1	tract					586:590	the gastrointestinal tract	565:590	the gastrointestinal tract	565:590	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	7	30	theme	imbalanced	986:995	arg1	equilibrium					997:1007	an imbalanced equilibrium	983:1007	an imbalanced equilibrium	983:1007	Several factors may disrupt the GIT microbiome's homeostasis leading to dysbiosis, characterized by an imbalanced equilibrium and perturbations in gut homeostasis.					
36270678	7	31	dep	disrupt	903:909	arg1	characterized					966:978	characterized	966:978	characterized by an imbalanced equilibrium and perturbations in gut homeostasis	966:1044	Several factors may disrupt the GIT microbiome's homeostasis leading to dysbiosis, characterized by an imbalanced equilibrium and perturbations in gut homeostasis.					
36270678	0	32	theme	liver	39:43	arg1	disease					45:51	non-alcoholic fatty liver disease	19:51	non-alcoholic fatty liver disease	19:51	Gut microbiome and non-alcoholic fatty liver disease.					
36270678	0	33	theme	fatty	33:37	arg1	disease					45:51	non-alcoholic fatty liver disease	19:51	non-alcoholic fatty liver disease	19:51	Gut microbiome and non-alcoholic fatty liver disease.					
36270678	1	34	contain	contains	93:100	arg1	GIT					88:90	GIT	88:90	GIT	88:90	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	1	34	contain	contains	93:100	arg1	tract					81:85	The human gastrointestinal tract	54:85	The human gastrointestinal tract (GIT)	54:91	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	1	34	contain	contains	93:100	arg2	collection					124:133	a dynamic and diverse collection	102:133	a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome	102:192	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	5	35	theme	fatty	750:754	arg1	NAFLD					771:775	NAFLD	771:775	NAFLD	771:775	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	5	35	theme	fatty	750:754	arg1	disease					762:768	non-alcoholic fatty liver disease	736:768	non-alcoholic fatty liver disease (NAFLD)	736:776	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	1	36	theme	gut	179:181	arg1	microbiome					183:192	the "gut microbiome	174:192	the "gut microbiome	174:192	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	5	37	theme	metabolic	711:719	arg1	disorders					721:729	metabolic disorders	711:729	metabolic disorders like non-alcoholic fatty liver disease (NAFLD)	711:776	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	9	38	theme	fecal	1269:1273	arg1	FMT					1303:1305	FMT	1303:1305	FMT	1303:1305	Recent evidence suggests that utilizing medications, prebiotics, probiotics, and fecal microbiota transplantation (FMT) to manipulate the microbiome could be a viable method for treating NAFLD.					
36270678	9	38	theme	fecal	1269:1273	arg1	transplantation					1286:1300	fecal microbiota transplantation	1269:1300	fecal microbiota transplantation (FMT)	1269:1306	Recent evidence suggests that utilizing medications, prebiotics, probiotics, and fecal microbiota transplantation (FMT) to manipulate the microbiome could be a viable method for treating NAFLD.					
36270678	8	39	theme	Irritable	1047:1055	arg1	IBD					1072:1074	IBD	1072:1074	IBD	1072:1074	Irritable bowel disease (IBD), malnutrition, and metabolic disorders, such as NAFLD, have been associated with the dysbiotic gut microbiome.					
36270678	8	39	theme	Irritable	1047:1055	arg1	disease					1063:1069	Irritable bowel disease	1047:1069	Irritable bowel disease (IBD)	1047:1075	Irritable bowel disease (IBD), malnutrition, and metabolic disorders, such as NAFLD, have been associated with the dysbiotic gut microbiome.					
36270678	5	40	theme	disorders	721:729	arg1	emergence					698:706	the emergence	694:706	the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD)	694:776	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	9	41	theme	microbiota	1275:1284	arg1	FMT					1303:1305	FMT	1303:1305	FMT	1303:1305	Recent evidence suggests that utilizing medications, prebiotics, probiotics, and fecal microbiota transplantation (FMT) to manipulate the microbiome could be a viable method for treating NAFLD.					
36270678	9	41	theme	microbiota	1275:1284	arg1	transplantation					1286:1300	fecal microbiota transplantation	1269:1300	fecal microbiota transplantation (FMT)	1269:1306	Recent evidence suggests that utilizing medications, prebiotics, probiotics, and fecal microbiota transplantation (FMT) to manipulate the microbiome could be a viable method for treating NAFLD.					
36270678	7	42	theme	GIT	915:917	arg1	microbiome					919:928	the GIT microbiome's	911:930	the GIT microbiome's homeostasis	911:942	Several factors may disrupt the GIT microbiome's homeostasis leading to dysbiosis, characterized by an imbalanced equilibrium and perturbations in gut homeostasis.					
36270678	1	43	theme	dynamic	104:110	arg1	collection					124:133	a dynamic and diverse collection	102:133	a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome	102:192	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	2	44	from	impact	227:232	arg1	host					241:244	the host	237:244	the host	237:244	The gut microbiome has a major impact on the host during homeostasis and disease.					
36270678	5	45	theme	host	619:622	arg1	health					624:629	host health	619:629	host health	619:629	According to new research, the microbes in the gastrointestinal tract play a substantial role in host health, and alterations in its composition and function might lead to the emergence of metabolic disorders like non-alcoholic fatty liver disease (NAFLD).					
36270678	1	46	theme	diverse	116:122	arg1	collection					124:133	a dynamic and diverse collection	102:133	a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome	102:192	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36270678	8	47	theme	gut	1172:1174	arg1	microbiome					1176:1185	the dysbiotic gut microbiome	1158:1185	the dysbiotic gut microbiome	1158:1185	Irritable bowel disease (IBD), malnutrition, and metabolic disorders, such as NAFLD, have been associated with the dysbiotic gut microbiome.					
36270678	8	48	theme	bowel	1057:1061	arg1	IBD					1072:1074	IBD	1072:1074	IBD	1072:1074	Irritable bowel disease (IBD), malnutrition, and metabolic disorders, such as NAFLD, have been associated with the dysbiotic gut microbiome.					
36270678	8	48	theme	bowel	1057:1061	arg1	disease					1063:1069	Irritable bowel disease	1047:1069	Irritable bowel disease (IBD)	1047:1075	Irritable bowel disease (IBD), malnutrition, and metabolic disorders, such as NAFLD, have been associated with the dysbiotic gut microbiome.					
36270678	2	49	theme	gut	200:202	arg1	microbiome					204:213	The gut microbiome	196:213	The gut microbiome	196:213	The gut microbiome has a major impact on the host during homeostasis and disease.					
36270678	1	50	dep	contains	93:100	arg1	"					194:194	"	194:194	"	194:194	The human gastrointestinal tract (GIT) contains a dynamic and diverse collection of bacteria, archaea, and fungi termed the "gut microbiome."					
36235218	5	0	dep	days	588:591	arg1	addition					603:610	the addition	599:610	the addition of each elicitor	599:627	Biomasses were collected at 24 h and 48 h, and 4, 6, and 8 days after the addition of each elicitor.					
36235218	10	1	theme	chemical	1480:1487	arg1	composition					1489:1499	the chemical composition	1476:1499	the chemical composition	1476:1499	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	4	2	theme	culture	520:526	arg1	day					513:515	the 10th day	504:515	the 10th day of culture	504:526	The elicitors were supplemented on the 10th day of culture.					
36235218	8	3	theme	total	1175:1179	arg1	content					1188:1194	the highest total lignan content	1163:1194	the highest total lignan content	1163:1194	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	8	4	theme	cultures	1132:1139	arg1	extracts					1118:1125	the biomass extracts	1106:1125	the biomass extracts of M cultures grown in bioreactors	1106:1160	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	6	5	theme	aryltetralin	679:690	arg1	lignans					729:735	the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans	652:735	the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans	652:735	The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans were determined qualitatively and quantitatively in biomass extracts using the UHPLC-MS/MS method.					
36235218	9	6	theme	agitated	1317:1324	arg1	cultures					1341:1348	agitated and bioreactor cultures	1317:1348	agitated and bioreactor cultures	1317:1348	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	7	7	theme	YeE	959:961	arg1	104.30					963:968	YeE 104.30	959:968	YeE 104.30 (F, day 8)	959:979	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	8	8	theme	M	1130:1130	arg1	cultures					1132:1139	M cultures	1130:1139	M cultures grown in bioreactors	1130:1160	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	9	9	theme	maximum	1258:1264	arg1	3.29					1355:1358	3.29	1355:1358	3.29	1355:1358	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	9	9	theme	maximum	1258:1264	arg1	contents					1279:1286	The maximum total lignan contents	1254:1286	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures	1254:1348	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	1	10	theme	methyl	236:241	arg1	jasmonate					243:251	methyl jasmonate	236:251	methyl jasmonate	236:251	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	7	11	theme	lignans	895:901	arg1	contents					869:876	The highest total contents	851:876	The highest total contents [mg/100 g DW] of lignans	851:901	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	1	12	theme	agitated	297:304	arg1	systems					352:358	Plantform temporary immersion systems	322:358	Plantform temporary immersion systems	322:358	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	12	theme	agitated	297:304	arg1	cultures					372:379	agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures	297:379	cultures	372:379	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	10	13	from	field	1532:1536	arg1	understanding					1459:1471	The poor understanding	1450:1471	The poor understanding of the chemical composition	1450:1499	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	10	13	from	field	1532:1536	arg1	lack					1509:1512	the lack	1505:1512	the lack of studies in the field of plant biotechnology of S. rubriflora	1505:1576	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	10	14	theme	biotechnology	1547:1559	arg1	field					1532:1536	the field	1528:1536	the field of plant biotechnology of S. rubriflora	1528:1576	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	9	15	theme	total	1266:1270	arg1	3.29					1355:1358	3.29	1355:1358	3.29	1355:1358	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	9	15	theme	total	1266:1270	arg1	contents					1279:1286	The maximum total lignan contents	1254:1286	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures	1254:1348	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	6	16	theme	dibenzocyclooctadiene	656:676	arg1	lignans					729:735	the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans	652:735	the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans	652:735	The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans were determined qualitatively and quantitatively in biomass extracts using the UHPLC-MS/MS method.					
36235218	7	17	theme	MeJA	1060:1063	arg1	89.70					1065:1069	MeJA 89.70	1060:1069	MeJA 89.70 (F, 48 h)	1060:1079	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	9	18	theme	lignan	1272:1277	arg1	3.29					1355:1358	3.29	1355:1358	3.29	1355:1358	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	9	18	theme	lignan	1272:1277	arg1	contents					1279:1286	The maximum total lignan contents	1254:1286	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures	1254:1348	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	7	19	dep	CH-95.00	913:920	arg1	F					923:923	F	923:923	F	923:923	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	7	19	dep	CH-95.00	913:920	arg1	day					926:928	day 6	926:930	day 6	926:930	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	1	20	theme	bioreactor	310:319	arg1	systems					352:358	Plantform temporary immersion systems	322:358	Plantform temporary immersion systems	322:358	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	20	theme	bioreactor	310:319	arg1	cultures					372:379	agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures	297:379	cultures	372:379	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	10	21	from	lack	1509:1512	arg1	field					1532:1536	the field	1528:1536	the field of plant biotechnology of S. rubriflora	1528:1576	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	9	22	from	cultures	1440:1447	arg1	extracts					1409:1416	the extracts	1405:1416	the extracts from the non-elicited cultures	1405:1447	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	5	23	theme	elicitor	620:627	arg1	addition					603:610	the addition	599:610	the addition of each elicitor	599:627	Biomasses were collected at 24 h and 48 h, and 4, 6, and 8 days after the addition of each elicitor.					
36235218	0	24	theme	rubriflora	47:56	arg1	Cultures					67:74	Schisandra rubriflora In Vitro Cultures	36:74	Schisandra rubriflora In Vitro Cultures	36:74	Stimulation of Lignan Production in Schisandra rubriflora In Vitro Cultures by Elicitation.					
36235218	1	25	theme	male	399:402	arg1	systems					352:358	Plantform temporary immersion systems	322:358	Plantform temporary immersion systems	322:358	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	25	theme	male	399:402	arg1	cultures					372:379	agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures	297:379	cultures	372:379	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	26	theme	Plantform	322:330	arg1	systems					352:358	Plantform temporary immersion systems	322:358	Plantform temporary immersion systems	322:358	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	26	theme	Plantform	322:330	arg1	cultures					372:379	agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures	297:379	cultures	372:379	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	9	27	from	3.29	1355:1358	arg1	extracts					1409:1416	the extracts	1405:1416	the extracts from the non-elicited cultures	1405:1447	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	8	28	theme	MeJA	1215:1218	arg1	DW					1249:1250	153.20 mg/100 g DW	1233:1250	153.20 mg/100 g DW	1233:1250	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	8	28	theme	MeJA	1215:1218	arg1	elicitation					1220:1230	MeJA elicitation	1215:1230	MeJA elicitation (153.20 mg/100 g DW)	1215:1251	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	7	29	theme	DW	888:889	arg1	contents					869:876	The highest total contents	851:876	The highest total contents [mg/100 g DW] of lignans	851:901	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	1	30	theme	temporary	332:340	arg1	systems					352:358	Plantform temporary immersion systems	322:358	Plantform temporary immersion systems	322:358	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	30	theme	temporary	332:340	arg1	cultures					372:379	agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures	297:379	cultures	372:379	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	10	31	from	understanding	1459:1471	arg1	field					1532:1536	the field	1528:1536	the field of plant biotechnology of S. rubriflora	1528:1576	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	8	32	theme	153.20	1233:1238	arg1	DW					1249:1250	153.20 mg/100 g DW	1233:1250	153.20 mg/100 g DW	1233:1250	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	8	32	theme	153.20	1233:1238	arg1	elicitation					1220:1230	MeJA elicitation	1215:1230	MeJA elicitation (153.20 mg/100 g DW)	1215:1251	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	0	33	theme	Production	22:31	arg1	Stimulation					0:10	Stimulation	0:10	Stimulation of Lignan Production in Schisandra rubriflora In Vitro Cultures by Elicitation.	0:90	Stimulation of Lignan Production in Schisandra rubriflora In Vitro Cultures by Elicitation.					
36235218	6	34	from	neolignans	741:750	arg1	compounds					637:645	The 24 compounds	630:645	The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans	630:750	The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans were determined qualitatively and quantitatively in biomass extracts using the UHPLC-MS/MS method.					
36235218	1	35	theme	immersion	342:350	arg1	systems					352:358	Plantform temporary immersion systems	322:358	Plantform temporary immersion systems	322:358	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	35	theme	immersion	342:350	arg1	cultures					372:379	agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures	297:379	cultures	372:379	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	7	36	dep	104.30	963:968	arg1	day					974:976	day 8	974:978	day 8	974:978	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	7	36	dep	104.30	963:968	arg1	F					971:971	F	971:971	F	971:971	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	9	37	dep	3.29	1355:1358	arg1	higher					1375:1380	higher	1375:1380	higher	1375:1380	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	9	38	theme	bioreactor	1330:1339	arg1	cultures					1341:1348	agitated and bioreactor cultures	1317:1348	agitated and bioreactor cultures	1317:1348	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	0	39	theme	Lignan	15:20	arg1	Production					22:31	Lignan Production	15:31	Lignan Production in Schisandra rubriflora In Vitro Cultures	15:74	Stimulation of Lignan Production in Schisandra rubriflora In Vitro Cultures by Elicitation.					
36235218	10	40	theme	studies	1517:1523	arg1	understanding					1459:1471	The poor understanding	1450:1471	The poor understanding of the chemical composition	1450:1499	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	10	40	theme	studies	1517:1523	arg1	lack					1509:1512	the lack	1505:1512	the lack of studies in the field of plant biotechnology of S. rubriflora	1505:1576	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	8	41	theme	mg/100	1240:1245	arg1	DW					1249:1250	153.20 mg/100 g DW	1233:1250	153.20 mg/100 g DW	1233:1250	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	8	41	theme	mg/100	1240:1245	arg1	elicitation					1220:1230	MeJA elicitation	1215:1230	MeJA elicitation (153.20 mg/100 g DW)	1215:1251	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	8	42	theme	g	1247:1247	arg1	DW					1249:1250	153.20 mg/100 g DW	1233:1250	153.20 mg/100 g DW	1233:1250	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	8	42	theme	g	1247:1247	arg1	elicitation					1220:1230	MeJA elicitation	1215:1230	MeJA elicitation (153.20 mg/100 g DW)	1215:1251	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	0	43	theme	Schisandra	36:45	arg1	Cultures					67:74	Schisandra rubriflora In Vitro Cultures	36:74	Schisandra rubriflora In Vitro Cultures	36:74	Stimulation of Lignan Production in Schisandra rubriflora In Vitro Cultures by Elicitation.					
36235218	7	44	dep	89.70	1065:1069	arg1	F					1072:1072	F	1072:1072	F	1072:1072	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	7	44	dep	89.70	1065:1069	arg1	h					1078:1078	48 h	1075:1078	48 h	1075:1078	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	7	45	theme	highest	855:861	arg1	contents					869:876	The highest total contents	851:876	The highest total contents [mg/100 g DW] of lignans	851:901	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	9	46	from	contents	1279:1286	arg1	cultures					1341:1348	agitated and bioreactor cultures	1317:1348	agitated and bioreactor cultures	1317:1348	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	9	46	from	contents	1279:1286	arg1	extracts					1303:1310	the biomass extracts	1291:1310	the biomass extracts from agitated and bioreactor cultures	1291:1348	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	1	47	dep	female	384:389	arg1	Rehd					430:433	Rehd	430:433	Rehd	430:433	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	7	48	dep	124.50	1012:1017	arg1	F					1020:1020	F	1020:1020	F	1020:1020	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	7	48	dep	124.50	1012:1017	arg1	h					1026:1026	48 h	1023:1026	48 h	1023:1026	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	7	49	theme	total	863:867	arg1	contents					869:876	The highest total contents	851:876	The highest total contents [mg/100 g DW] of lignans	851:901	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	0	50	from	Cultures	67:74	arg1	Stimulation					0:10	Stimulation	0:10	Stimulation of Lignan Production in Schisandra rubriflora In Vitro Cultures by Elicitation.	0:90	Stimulation of Lignan Production in Schisandra rubriflora In Vitro Cultures by Elicitation.					
36235218	9	51	theme	biomass	1295:1301	arg1	extracts					1303:1310	the biomass extracts	1291:1310	the biomass extracts from agitated and bioreactor cultures	1291:1348	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	9	52	theme	non-elicited	1427:1438	arg1	cultures					1440:1447	the non-elicited cultures	1423:1447	the non-elicited cultures	1423:1447	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	1	53	theme	yeast	173:177	arg1	YeE					188:190	YeE	188:190	YeE	188:190	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	53	theme	yeast	173:177	arg1	chitosan					147:154	chitosan	147:154	chitosan (CH) (200 mg/L)	147:170	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	53	theme	yeast	173:177	arg1	extract					179:185	yeast extract	173:185	yeast extract (YeE) (3000 mg/L)	173:203	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	53	theme	yeast	173:177	arg1	mg/L					199:202	3000 mg/L	194:202	3000 mg/L	194:202	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	0	54	theme	In	58:59	arg1	Cultures					67:74	Schisandra rubriflora In Vitro Cultures	36:74	Schisandra rubriflora In Vitro Cultures	36:74	Stimulation of Lignan Production in Schisandra rubriflora In Vitro Cultures by Elicitation.					
36235218	6	55	theme	tetrahydrofuran	713:727	arg1	lignans					729:735	the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans	652:735	the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans	652:735	The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans were determined qualitatively and quantitatively in biomass extracts using the UHPLC-MS/MS method.					
36235218	7	56	dep	323.30	937:942	arg1	M					945:945	M	945:945	M	945:945	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	7	56	dep	323.30	937:942	arg1	h					951:951	48 h	948:951	48 h	948:951	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	0	57	theme	Vitro	61:65	arg1	Cultures					67:74	Schisandra rubriflora In Vitro Cultures	36:74	Schisandra rubriflora In Vitro Cultures	36:74	Stimulation of Lignan Production in Schisandra rubriflora In Vitro Cultures by Elicitation.					
36235218	7	58	dep	334.90	1033:1038	arg1	day					1044:1046	day 4	1044:1048	day 4	1044:1048	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	7	58	dep	334.90	1033:1038	arg1	M					1041:1041	M	1041:1041	M	1041:1041	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	0	59	from	Stimulation	0:10	arg1	Cultures					67:74	Schisandra rubriflora In Vitro Cultures	36:74	Schisandra rubriflora In Vitro Cultures	36:74	Stimulation of Lignan Production in Schisandra rubriflora In Vitro Cultures by Elicitation.					
36235218	6	60	theme	dibenzylbutane	693:706	arg1	lignans					729:735	the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans	652:735	the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans	652:735	The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans were determined qualitatively and quantitatively in biomass extracts using the UHPLC-MS/MS method.					
36235218	1	61	theme	microshoot	361:370	arg1	systems					352:358	Plantform temporary immersion systems	322:358	Plantform temporary immersion systems	322:358	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	61	theme	microshoot	361:370	arg1	cultures					372:379	agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures	297:379	cultures	372:379	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	10	62	theme	rubriflora	1567:1576	arg1	field					1532:1536	the field	1528:1536	the field of plant biotechnology of S. rubriflora	1528:1576	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	10	63	theme	plant	1541:1545	arg1	biotechnology					1547:1559	plant biotechnology	1541:1559	plant biotechnology	1541:1559	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	10	64	theme	poor	1454:1457	arg1	understanding					1459:1471	The poor understanding	1450:1471	The poor understanding of the chemical composition	1450:1499	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	6	65	from	lignans	729:735	arg1	compounds					637:645	The 24 compounds	630:645	The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans	630:750	The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans were determined qualitatively and quantitatively in biomass extracts using the UHPLC-MS/MS method.					
36235218	3	66	theme	Schisandraceae	446:459	arg1	lines					462:466	(Schisandraceae) lines	445:466	(Schisandraceae) lines.	445:467	(Schisandraceae) lines.					
36235218	10	67	theme	research	1614:1621	arg1	innovativeness					1592:1605	the innovativeness	1588:1605	the innovativeness of the research	1588:1621	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	9	68	from	cultures	1341:1348	arg1	3.29					1355:1358	3.29	1355:1358	3.29	1355:1358	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	9	68	from	cultures	1341:1348	arg1	extracts					1303:1310	the biomass extracts	1291:1310	the biomass extracts from agitated and bioreactor cultures	1291:1348	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	9	68	from	cultures	1341:1348	arg1	contents					1279:1286	The maximum total lignan contents	1254:1286	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures	1254:1348	The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures.					
36235218	8	69	theme	biomass	1110:1116	arg1	extracts					1118:1125	the biomass extracts	1106:1125	the biomass extracts of M cultures grown in bioreactors	1106:1160	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	10	70	from	studies	1517:1523	arg1	field					1532:1536	the field	1528:1536	the field of plant biotechnology of S. rubriflora	1528:1576	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	1	71	theme	lignan	274:279	arg1	accumulation					281:292	lignan accumulation	274:292	lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd	274:433	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	7	72	theme	ETH	1008:1010	arg1	124.50					1012:1017	ETH 124.50	1008:1017	ETH 124.50 (F, 48 h)	1008:1027	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	1	73	theme	elicitation	129:139	arg1	effect					119:124	the effect	115:124	the effect of elicitation	115:139	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	8	74	theme	highest	1167:1173	arg1	content					1188:1194	the highest total lignan content	1163:1194	the highest total lignan content	1163:1194	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	6	75	theme	UHPLC-MS/MS	831:841	arg1	method					843:848	the UHPLC-MS/MS method	827:848	the UHPLC-MS/MS method	827:848	The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans were determined qualitatively and quantitatively in biomass extracts using the UHPLC-MS/MS method.					
36235218	7	76	dep	353.17	985:990	arg1	day					996:998	day 4	996:1000	day 4	996:1000	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	7	76	dep	353.17	985:990	arg1	M					993:993	M	993:993	M	993:993	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	1	77	theme	female	384:389	arg1	systems					352:358	Plantform temporary immersion systems	322:358	Plantform temporary immersion systems	322:358	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	77	theme	female	384:389	arg1	cultures					372:379	agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures	297:379	cultures	372:379	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	10	78	theme	composition	1489:1499	arg1	understanding					1459:1471	The poor understanding	1450:1471	The poor understanding of the chemical composition	1450:1499	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	10	78	theme	composition	1489:1499	arg1	lack					1509:1512	the lack	1505:1512	the lack of studies in the field of plant biotechnology of S. rubriflora	1505:1576	The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.					
36235218	1	79	from	accumulation	281:292	arg1	systems					352:358	Plantform temporary immersion systems	322:358	Plantform temporary immersion systems	322:358	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	1	79	from	accumulation	281:292	arg1	cultures					372:379	agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures	297:379	cultures	372:379	The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd.					
36235218	0	80	from	Production	22:31	arg1	Cultures					67:74	Schisandra rubriflora In Vitro Cultures	36:74	Schisandra rubriflora In Vitro Cultures	36:74	Stimulation of Lignan Production in Schisandra rubriflora In Vitro Cultures by Elicitation.					
36235218	7	81	dep	368.50	1085:1090	arg1	M					1093:1093	M	1093:1093	M	1093:1093	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	7	81	dep	368.50	1085:1090	arg1	h					1099:1099	24 h	1096:1099	24 h	1096:1099	The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h).					
36235218	4	82	theme	10th	508:511	arg1	day					513:515	the 10th day	504:515	the 10th day of culture	504:526	The elicitors were supplemented on the 10th day of culture.					
36235218	8	83	theme	lignan	1181:1186	arg1	content					1188:1194	the highest total lignan content	1163:1194	the highest total lignan content	1163:1194	In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW).					
36235218	6	84	theme	biomass	804:810	arg1	extracts					812:819	biomass extracts	804:819	biomass extracts	804:819	The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans were determined qualitatively and quantitatively in biomass extracts using the UHPLC-MS/MS method.					
36550362	3	0	theme	SARS-CoV-2	337:346	arg1	results					357:363	False positive SARS-CoV-2 antibody results	322:363	False positive SARS-CoV-2 antibody results	322:363	False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries.					
36550362	5	1	theme	malaria	717:723	arg1	infection					725:733	acute malaria infection	711:733	acute malaria infection	711:733	Individuals with acute malaria infection generated substantial SARS-CoV-2 reactivity.					
36550362	8	2	theme	cross-reactive	1122:1135	arg1	antibodies					1137:1146	cross-reactive antibodies	1122:1146	cross-reactive antibodies measured by neutralization assays	1122:1180	The functional activity of cross-reactive antibodies measured by neutralization assays showed that cross-reactive antibodies did not neutralize SARS-CoV-2 in vitro.					
36550362	10	3	from	risk	1626:1629	arg1	Africa					1681:1686	Africa	1681:1686	Africa	1681:1686	Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.					
36550362	5	4	theme	SARS-CoV-2	757:766	arg1	reactivity					768:777	substantial SARS-CoV-2 reactivity	745:777	substantial SARS-CoV-2 reactivity	745:777	Individuals with acute malaria infection generated substantial SARS-CoV-2 reactivity.					
36550362	10	5	theme	transmission	1653:1664	arg1	risk					1626:1629	risk	1626:1629	risk of continued COVID-19 transmission in sub-Saharan Africa	1626:1686	Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.					
36550362	3	6	theme	antibody	348:355	arg1	results					357:363	False positive SARS-CoV-2 antibody results	322:363	False positive SARS-CoV-2 antibody results	322:363	False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries.					
36550362	10	7	theme	COVID-19	1644:1651	arg1	transmission					1653:1664	continued COVID-19 transmission	1634:1664	continued COVID-19 transmission	1634:1664	Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.					
36550362	6	8	theme	protein	918:924	arg1	arrays					926:931	protein arrays	918:931	protein arrays	918:931	Cross-reactivity was not associated with reactivity to other human coronaviruses or other SARS-CoV-2 proteins, as measured by peptide and protein arrays.					
36550362	9	9	theme	antibody	1355:1362	arg1	results					1364:1370	false positive SARS-CoV-2 antibody results	1329:1370	false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity	1329:1462	Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity.					
36550362	7	10	theme	S1	982:983	arg1	subunits					985:992	desialated Spike S1 subunits	965:992	desialated Spike S1 subunits	965:992	ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein.					
36550362	8	11	theme	neutralization	1160:1173	arg1	assays					1175:1180	neutralization assays	1160:1180	neutralization assays	1160:1180	The functional activity of cross-reactive antibodies measured by neutralization assays showed that cross-reactive antibodies did not neutralize SARS-CoV-2 in vitro.					
36550362	0	12	theme	Spike	109:113	arg1	protein					115:121	the SARS-CoV-2 Spike protein	94:121	the SARS-CoV-2 Spike protein	94:121	Plasmodium infection is associated with cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.					
36550362	4	13	theme	endemic	568:574	arg1	countries					592:600	eight malaria endemic and non-endemic countries	554:600	eight malaria endemic and non-endemic countries	554:600	Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity.					
36550362	3	14	dep	understanding	407:419	arg1	association					426:436	this association	421:436	understanding this association	407:436	False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries.					
36550362	7	15	theme	protein	1086:1092	arg1	glycans					1065:1071	N-linked glycans	1056:1071	N-linked glycans of the Spike protein	1056:1092	ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein.					
36550362	2	16	theme	sero-surveillance	298:314	arg1	efficacy					284:291	their efficacy	278:291	their efficacy	278:291	However, SARS-CoV-2 antibody test results can produce false positive results, limiting their efficacy as a sero-surveillance tool.					
36550362	2	16	theme	sero-surveillance	298:314	arg1	tool					316:319	a sero-surveillance tool	296:319	a sero-surveillance tool	296:319	However, SARS-CoV-2 antibody test results can produce false positive results, limiting their efficacy as a sero-surveillance tool.					
36550362	4	17	theme	SARS-CoV-2	654:663	arg1	reactivity					682:691	SARS-CoV-2 Spike S1 subunit reactivity	654:691	SARS-CoV-2 Spike S1 subunit reactivity	654:691	Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity.					
36550362	0	18	theme	SARS-CoV-2	98:107	arg1	protein					115:121	the SARS-CoV-2 Spike protein	94:121	the SARS-CoV-2 Spike protein	94:121	Plasmodium infection is associated with cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.					
36550362	4	19	theme	pre-pandemic	528:539	arg1	samples					541:547	pre-pandemic samples	528:547	pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents	528:620	Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity.					
36550362	5	20	theme	substantial	745:755	arg1	reactivity					768:777	substantial SARS-CoV-2 reactivity	745:777	substantial SARS-CoV-2 reactivity	745:777	Individuals with acute malaria infection generated substantial SARS-CoV-2 reactivity.					
36550362	7	21	theme	desialated	965:974	arg1	subunits					985:992	desialated Spike S1 subunits	965:992	desialated Spike S1 subunits	965:992	ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein.					
36550362	9	22	from	results	1364:1370	arg1	regions					1391:1397	malaria endemic regions	1375:1397	malaria endemic regions	1375:1397	Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity.					
36550362	8	23	theme	cross-reactive	1194:1207	arg1	antibodies					1209:1218	cross-reactive antibodies	1194:1218	cross-reactive antibodies	1194:1218	The functional activity of cross-reactive antibodies measured by neutralization assays showed that cross-reactive antibodies did not neutralize SARS-CoV-2 in vitro.					
36550362	8	24	theme	functional	1099:1108	arg1	activity					1110:1117	The functional activity	1095:1117	The functional activity of cross-reactive antibodies measured by neutralization assays	1095:1180	The functional activity of cross-reactive antibodies measured by neutralization assays showed that cross-reactive antibodies did not neutralize SARS-CoV-2 in vitro.					
36550362	7	25	theme	Spike	976:980	arg1	subunits					985:992	desialated Spike S1 subunits	965:992	desialated Spike S1 subunits	965:992	ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein.					
36550362	9	26	theme	assays	1306:1311	arg1	use					1274:1276	routine use	1266:1276	routine use of glycosylated or sialated assays	1266:1311	Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity.					
36550362	6	27	theme	other	864:868	arg1	proteins					881:888	other SARS-CoV-2 proteins	864:888	other SARS-CoV-2 proteins	864:888	Cross-reactivity was not associated with reactivity to other human coronaviruses or other SARS-CoV-2 proteins, as measured by peptide and protein arrays.					
36550362	10	28	theme	risk	1626:1629	arg1	underestimates					1608:1621	underestimates	1608:1621	underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa	1608:1686	Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.					
36550362	0	29	theme	Plasmodium	0:9	arg1	infection					11:19	Plasmodium infection	0:19	Plasmodium infection	0:19	Plasmodium infection is associated with cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.					
36550362	5	30	theme	acute	711:715	arg1	infection					725:733	acute malaria infection	711:733	acute malaria infection	711:733	Individuals with acute malaria infection generated substantial SARS-CoV-2 reactivity.					
36550362	10	31	theme	population-level	1554:1569	arg1	exposure					1571:1578	population-level exposure	1554:1578	population-level exposure to SARS-CoV-2	1554:1592	Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.					
36550362	3	32	theme	sero-surveillance	464:480	arg1	results					482:488	sero-surveillance results	464:488	sero-surveillance results from malaria-endemic countries	464:519	False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries.					
36550362	9	33	theme	sialated	1297:1304	arg1	assays					1306:1311	sialated assays	1297:1311	sialated assays	1297:1311	Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity.					
36550362	10	34	theme	continued	1634:1642	arg1	transmission					1653:1664	continued COVID-19 transmission	1634:1664	continued COVID-19 transmission	1634:1664	Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.					
36550362	8	35	theme	antibodies	1137:1146	arg1	activity					1110:1117	The functional activity	1095:1117	The functional activity of cross-reactive antibodies measured by neutralization assays	1095:1180	The functional activity of cross-reactive antibodies measured by neutralization assays showed that cross-reactive antibodies did not neutralize SARS-CoV-2 in vitro.					
36550362	7	36	theme	Spike	1080:1084	arg1	protein					1086:1092	the Spike protein	1076:1092	the Spike protein	1076:1092	ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein.					
36550362	10	37	from	underestimates	1608:1621	arg1	Africa					1681:1686	Africa	1681:1686	Africa	1681:1686	Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.					
36550362	4	38	from	continents	611:620	arg1	samples					541:547	pre-pandemic samples	528:547	pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents	528:620	Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity.					
36550362	9	39	theme	endemic	1383:1389	arg1	regions					1391:1397	malaria endemic regions	1375:1397	malaria endemic regions	1375:1397	Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity.					
36550362	10	40	from	Africa	1681:1686	arg1	underestimates					1608:1621	underestimates	1608:1621	underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa	1608:1686	Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.					
36550362	9	41	theme	population-level	1438:1453	arg1	immunity					1455:1462	population-level immunity	1438:1462	population-level immunity	1438:1462	Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity.					
36550362	0	42	theme	cross-reactive	40:53	arg1	antibodies					55:64	cross-reactive antibodies	40:64	cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein	40:121	Plasmodium infection is associated with cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.					
36550362	2	43	theme	positive	251:258	arg1	results					260:266	false positive results	245:266	false positive results	245:266	However, SARS-CoV-2 antibody test results can produce false positive results, limiting their efficacy as a sero-surveillance tool.					
36550362	9	44	theme	routine	1266:1272	arg1	use					1274:1276	routine use	1266:1276	routine use of glycosylated or sialated assays	1266:1311	Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity.					
36550362	3	45	theme	malaria-endemic	495:509	arg1	countries					511:519	malaria-endemic countries	495:519	malaria-endemic countries	495:519	False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries.					
36550362	0	46	from	epitopes	82:89	arg1	protein					115:121	the SARS-CoV-2 Spike protein	94:121	the SARS-CoV-2 Spike protein	94:121	Plasmodium infection is associated with cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.					
36550362	2	47	theme	false	245:249	arg1	results					260:266	false positive results	245:266	false positive results	245:266	However, SARS-CoV-2 antibody test results can produce false positive results, limiting their efficacy as a sero-surveillance tool.					
36550362	6	48	theme	SARS-CoV-2	870:879	arg1	proteins					881:888	other SARS-CoV-2 proteins	864:888	other SARS-CoV-2 proteins	864:888	Cross-reactivity was not associated with reactivity to other human coronaviruses or other SARS-CoV-2 proteins, as measured by peptide and protein arrays.					
36550362	9	49	theme	SARS-CoV-2	1344:1353	arg1	results					1364:1370	false positive SARS-CoV-2 antibody results	1329:1370	false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity	1329:1462	Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity.					
36550362	4	50	from	countries	592:600	arg1	samples					541:547	pre-pandemic samples	528:547	pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents	528:620	Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity.					
36550362	3	51	theme	malaria	385:391	arg1	exposure					393:400	malaria exposure	385:400	malaria exposure	385:400	False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries.					
36550362	3	52	theme	False	322:326	arg1	results					357:363	False positive SARS-CoV-2 antibody results	322:363	False positive SARS-CoV-2 antibody results	322:363	False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries.					
36550362	3	53	from	countries	511:519	arg1	results					482:488	sero-surveillance results	464:488	sero-surveillance results from malaria-endemic countries	464:519	False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries.					
36550362	7	54	from	acid	1048:1051	arg1	glycans					1065:1071	N-linked glycans	1056:1071	N-linked glycans of the Spike protein	1056:1092	ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein.					
36550362	3	55	theme	positive	328:335	arg1	results					357:363	False positive SARS-CoV-2 antibody results	322:363	False positive SARS-CoV-2 antibody results	322:363	False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries.					
36550362	7	56	theme	N-linked	1056:1063	arg1	glycans					1065:1071	N-linked glycans	1056:1071	N-linked glycans of the Spike protein	1056:1092	ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein.					
36550362	9	57	theme	glycosylated	1281:1292	arg1	use					1274:1276	routine use	1266:1276	routine use of glycosylated or sialated assays	1266:1311	Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity.					
36550362	7	58	with	ELISAs	934:939	arg1	subunits					985:992	desialated Spike S1 subunits	965:992	desialated Spike S1 subunits	965:992	ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein.					
36550362	7	58	with	ELISAs	934:939	arg1	deglycosylated					946:959	deglycosylated	946:959	deglycosylated	946:959	ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein.					
36550362	9	59	theme	malaria	1375:1381	arg1	regions					1391:1397	malaria endemic regions	1375:1397	malaria endemic regions	1375:1397	Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity.					
36550362	2	60	theme	test	220:223	arg1	results					225:231	SARS-CoV-2 antibody test results	200:231	SARS-CoV-2 antibody test results	200:231	However, SARS-CoV-2 antibody test results can produce false positive results, limiting their efficacy as a sero-surveillance tool.					
36550362	9	61	theme	false	1329:1333	arg1	results					1364:1370	false positive SARS-CoV-2 antibody results	1329:1370	false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity	1329:1462	Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity.					
36550362	7	62	theme	sialic	1041:1046	arg1	acid					1048:1051	sialic acid	1041:1051	sialic acid on N-linked glycans of the Spike protein	1041:1092	ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein.					
36550362	4	63	theme	S1	671:672	arg1	reactivity					682:691	SARS-CoV-2 Spike S1 subunit reactivity	654:691	SARS-CoV-2 Spike S1 subunit reactivity	654:691	Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity.					
36550362	2	64	theme	antibody	211:218	arg1	results					225:231	SARS-CoV-2 antibody test results	200:231	SARS-CoV-2 antibody test results	200:231	However, SARS-CoV-2 antibody test results can produce false positive results, limiting their efficacy as a sero-surveillance tool.					
36550362	6	65	theme	human	841:845	arg1	coronaviruses					847:859	other human coronaviruses	835:859	other human coronaviruses	835:859	Cross-reactivity was not associated with reactivity to other human coronaviruses or other SARS-CoV-2 proteins, as measured by peptide and protein arrays.					
36550362	5	66	with	Individuals	694:704	arg1	infection					725:733	acute malaria infection	711:733	acute malaria infection	711:733	Individuals with acute malaria infection generated substantial SARS-CoV-2 reactivity.					
36550362	7	67	theme	cross-reactive	1008:1021	arg1	antibodies					1023:1032	cross-reactive antibodies	1008:1032	cross-reactive antibodies	1008:1032	ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein.					
36550362	4	68	theme	Spike	665:669	arg1	reactivity					682:691	SARS-CoV-2 Spike S1 subunit reactivity	654:691	SARS-CoV-2 Spike S1 subunit reactivity	654:691	Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity.					
36550362	4	69	theme	non-endemic	580:590	arg1	countries					592:600	eight malaria endemic and non-endemic countries	554:600	eight malaria endemic and non-endemic countries	554:600	Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity.					
36550362	2	70	theme	SARS-CoV-2	200:209	arg1	results					225:231	SARS-CoV-2 antibody test results	200:231	SARS-CoV-2 antibody test results	200:231	However, SARS-CoV-2 antibody test results can produce false positive results, limiting their efficacy as a sero-surveillance tool.					
36550362	6	71	theme	other	835:839	arg1	coronaviruses					847:859	other human coronaviruses	835:859	other human coronaviruses	835:859	Cross-reactivity was not associated with reactivity to other human coronaviruses or other SARS-CoV-2 proteins, as measured by peptide and protein arrays.					
36550362	1	72	theme	project	158:164	arg1	burden					174:179	project disease burden	158:179	project disease burden	158:179	Sero-surveillance can monitor and project disease burden and risk.					
36550362	9	73	dep	false	1329:1333	arg1	positive					1335:1342	positive	1335:1342	positive	1335:1342	Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity.					
36550362	7	74	link	N-linked	1056:1063	arg1	glycans					1065:1071	N-linked glycans	1056:1071	N-linked glycans of the Spike protein	1056:1092	ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein.					
36550362	0	75	theme	carbohydrate	69:80	arg1	epitopes					82:89	carbohydrate epitopes	69:89	carbohydrate epitopes on the SARS-CoV-2 Spike protein	69:121	Plasmodium infection is associated with cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.					
36550362	4	76	theme	malaria	560:566	arg1	countries					592:600	eight malaria endemic and non-endemic countries	554:600	eight malaria endemic and non-endemic countries	554:600	Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity.					
36550362	1	77	theme	disease	166:172	arg1	burden					174:179	project disease burden	158:179	project disease burden	158:179	Sero-surveillance can monitor and project disease burden and risk.					
36550362	4	78	theme	subunit	674:680	arg1	reactivity					682:691	SARS-CoV-2 Spike S1 subunit reactivity	654:691	SARS-CoV-2 Spike S1 subunit reactivity	654:691	Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity.					
35614038	4	0	theme	cell	728:731	arg1	clusters					733:740	phenotypically distinct immune cell clusters	697:740	phenotypically distinct immune cell clusters	697:740	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	1	1	theme	sulfate	165:171	arg1	inhibitors					209:218	potent inhibitors	202:218	potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI)	202:286	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	1	1	theme	sulfate	165:171	arg1	CSPGs					188:192	CSPGs	188:192	CSPGs	188:192	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	1	1	theme	sulfate	165:171	arg1	proteoglycans					173:185	Chondroitin sulfate proteoglycans	153:185	Chondroitin sulfate proteoglycans (CSPGs)	153:193	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	5	2	from	phenotype	1034:1042	arg1	cells					1065:1069	pro-repair immune cells	1047:1069	pro-repair immune cells	1047:1069	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	6	3	theme	critical	1217:1224	arg1	CSPGs					1208:1212	CSPGs	1208:1212	CSPGs	1208:1212	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	6	3	theme	critical	1217:1224	arg1	mediators					1226:1234	critical mediators	1217:1234	critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction	1217:1375	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	6	4	from	rodents	1284:1290	arg1	CSPGs					1208:1212	CSPGs	1208:1212	CSPGs	1208:1212	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	6	4	from	rodents	1284:1290	arg1	mediators					1226:1234	critical mediators	1217:1234	critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction	1217:1375	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	6	5	theme	tissue	1358:1363	arg1	destruction					1365:1375	irreversible tissue destruction	1345:1375	irreversible tissue destruction	1345:1375	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	2	6	theme	critical	327:334	arg1	role					336:339	a critical role	325:339	a critical role	325:339	Here we reveal that CSPGs also play a critical role in preventing inflammation resolution by blocking the conversion of pro-inflammatory immune cells to a pro-repair phenotype in rodent models of SCI.					
35614038	1	7	theme	spinal	263:268	arg1	SCI					283:285	SCI	283:285	SCI	283:285	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	1	7	theme	spinal	263:268	arg1	injury					275:280	spinal cord injury	263:280	spinal cord injury (SCI)	263:286	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	6	8	theme	irreversible	1345:1356	arg1	destruction					1365:1375	irreversible tissue destruction	1345:1375	irreversible tissue destruction	1345:1375	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	3	9	theme	CSPG	533:536	arg1	glycosaminoglycans					538:555	CSPG glycosaminoglycans	533:555	CSPG glycosaminoglycans	533:555	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	1	10	theme	cord	270:273	arg1	SCI					283:285	SCI	283:285	SCI	283:285	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	1	10	theme	cord	270:273	arg1	injury					275:280	spinal cord injury	263:280	spinal cord injury (SCI)	263:286	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	4	11	theme	macrophage	779:788	arg1	subtypes					805:812	macrophage and microglial subtypes	779:812	macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI	779:976	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	4	12	theme	subtypes	805:812	arg1	modulation					765:774	CSPG-mediated modulation	751:774	CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI	751:976	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	3	13	theme	glycosaminoglycans	538:555	arg1	digestion					520:528	enzymatic digestion	510:528	enzymatic digestion of CSPG glycosaminoglycans	510:555	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	4	14	theme	CSPG-mediated	751:763	arg1	modulation					765:774	CSPG-mediated modulation	751:774	CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI	751:976	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	4	15	theme	immune	721:726	arg1	clusters					733:740	phenotypically distinct immune cell clusters	697:740	phenotypically distinct immune cell clusters	697:740	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	6	16	from	failure	1263:1269	arg1	rodents					1284:1290	rodents	1284:1290	rodents	1284:1290	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	5	17	theme	pro-inflammatory	1017:1032	arg1	phenotype					1034:1042	a pro-inflammatory phenotype	1015:1042	a pro-inflammatory phenotype in pro-repair immune cells	1015:1069	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	5	18	from	cells	1065:1069	arg1	activation					1001:1010	CSPG activation	996:1010	CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells	996:1069	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	5	19	theme	key	1136:1138	arg1	driver					1140:1145	a key driver	1134:1145	a key driver of CSPG-mediated immune modulation	1134:1180	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	5	19	theme	key	1136:1138	arg1	signalling					1120:1129	TLR4 signalling	1115:1129	TLR4 signalling	1115:1129	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	0	20	theme	rodent	116:121	arg1	models					123:128	rodent models	116:128	rodent models of spinal cord injury	116:150	Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury.					
35614038	5	21	theme	CSPG-mediated	1150:1162	arg1	modulation					1171:1180	CSPG-mediated immune modulation	1150:1180	CSPG-mediated immune modulation	1150:1180	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	2	22	theme	rodent	468:473	arg1	models					475:480	rodent models	468:480	rodent models of SCI	468:487	Here we reveal that CSPGs also play a critical role in preventing inflammation resolution by blocking the conversion of pro-inflammatory immune cells to a pro-repair phenotype in rodent models of SCI.					
35614038	5	23	theme	phenotype	1034:1042	arg1	activation					1001:1010	CSPG activation	996:1010	CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells	996:1069	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	0	24	theme	sulfate	12:18	arg1	proteoglycans					20:32	Chondroitin sulfate proteoglycans	0:32	Chondroitin sulfate proteoglycans	0:32	Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury.					
35614038	5	25	theme	immune	1164:1169	arg1	modulation					1171:1180	CSPG-mediated immune modulation	1150:1180	CSPG-mediated immune modulation	1150:1180	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	6	26	theme	prolonged	1308:1316	arg1	pathology					1331:1339	prolonged inflammatory pathology	1308:1339	prolonged inflammatory pathology	1308:1339	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	3	27	from	points	677:682	arg1	protein					617:623	pro-inflammatory protein and gene expression profiles	600:652	protein	617:623	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	3	27	from	points	677:682	arg1	profiles					645:652	pro-inflammatory protein and gene expression profiles	600:652	profiles	645:652	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	3	28	theme	cell	573:576	arg1	clearance					578:586	immune cell clearance	566:586	immune cell clearance	566:586	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	0	29	theme	Chondroitin	0:10	arg1	proteoglycans					20:32	Chondroitin sulfate proteoglycans	0:32	Chondroitin sulfate proteoglycans	0:32	Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury.					
35614038	5	30	theme	TLR4	1115:1118	arg1	driver					1140:1145	a key driver	1134:1145	a key driver of CSPG-mediated immune modulation	1134:1180	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	5	30	theme	TLR4	1115:1118	arg1	signalling					1120:1129	TLR4 signalling	1115:1129	TLR4 signalling	1115:1129	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	2	31	theme	inflammation	355:366	arg1	resolution					368:377	inflammation resolution	355:377	inflammation resolution	355:377	Here we reveal that CSPGs also play a critical role in preventing inflammation resolution by blocking the conversion of pro-inflammatory immune cells to a pro-repair phenotype in rodent models of SCI.					
35614038	2	32	theme	cells	433:437	arg1	conversion					395:404	the conversion	391:404	the conversion of pro-inflammatory immune cells to a pro-repair phenotype	391:463	Here we reveal that CSPGs also play a critical role in preventing inflammation resolution by blocking the conversion of pro-inflammatory immune cells to a pro-repair phenotype in rodent models of SCI.					
35614038	0	33	theme	spinal	133:138	arg1	injury					145:150	spinal cord injury	133:150	spinal cord injury	133:150	Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury.					
35614038	4	34	theme	microglial	794:803	arg1	subtypes					805:812	macrophage and microglial subtypes	779:812	macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI	779:976	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	4	35	theme	composition	865:875	arg1	changes					877:883	composition changes	865:883	composition changes	865:883	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	2	36	theme	pro-inflammatory	409:424	arg1	cells					433:437	pro-inflammatory immune cells	409:437	pro-inflammatory immune cells	409:437	Here we reveal that CSPGs also play a critical role in preventing inflammation resolution by blocking the conversion of pro-inflammatory immune cells to a pro-repair phenotype in rodent models of SCI.					
35614038	2	37	theme	immune	426:431	arg1	cells					433:437	pro-inflammatory immune cells	409:437	pro-inflammatory immune cells	409:437	Here we reveal that CSPGs also play a critical role in preventing inflammation resolution by blocking the conversion of pro-inflammatory immune cells to a pro-repair phenotype in rodent models of SCI.					
35614038	6	38	theme	resolution	1252:1261	arg1	failure					1263:1269	inflammation resolution failure	1239:1269	inflammation resolution failure	1239:1269	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	3	39	theme	resolution	661:670	arg1	points					677:682	key resolution time points	657:682	key resolution time points	657:682	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	3	40	theme	immune	566:571	arg1	clearance					578:586	immune cell clearance	566:586	immune cell clearance	566:586	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	1	41	theme	potent	202:207	arg1	inhibitors					209:218	potent inhibitors	202:218	potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI)	202:286	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	1	41	theme	potent	202:207	arg1	proteoglycans					173:185	Chondroitin sulfate proteoglycans	153:185	Chondroitin sulfate proteoglycans (CSPGs)	153:193	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	0	42	theme	cell	49:52	arg1	conversion					65:74	immune cell phenotypic conversion	42:74	immune cell phenotypic conversion	42:74	Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury.					
35614038	6	43	from	mediators	1226:1234	arg1	rodents					1284:1290	rodents	1284:1290	rodents	1284:1290	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	0	44	theme	injury	145:150	arg1	models					123:128	rodent models	116:128	rodent models of spinal cord injury	116:150	Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury.					
35614038	3	45	theme	time	672:675	arg1	points					677:682	key resolution time points	657:682	key resolution time points	657:682	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	3	46	theme	pro-inflammatory	600:615	arg1	protein					617:623	pro-inflammatory protein and gene expression profiles	600:652	protein	617:623	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	6	47	theme	failure	1263:1269	arg1	CSPGs					1208:1212	CSPGs	1208:1212	CSPGs	1208:1212	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	6	47	theme	failure	1263:1269	arg1	mediators					1226:1234	critical mediators	1217:1234	critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction	1217:1375	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	0	48	theme	immune	42:47	arg1	conversion					65:74	immune cell phenotypic conversion	42:74	immune cell phenotypic conversion	42:74	Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury.					
35614038	0	49	theme	cord	140:143	arg1	injury					145:150	spinal cord injury	133:150	spinal cord injury	133:150	Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury.					
35614038	3	50	theme	key	657:659	arg1	points					677:682	key resolution time points	657:682	key resolution time points	657:682	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	0	51	theme	phenotypic	54:63	arg1	conversion					65:74	immune cell phenotypic conversion	42:74	immune cell phenotypic conversion	42:74	Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury.					
35614038	6	52	theme	inflammation	1239:1250	arg1	failure					1263:1269	inflammation resolution failure	1239:1269	inflammation resolution failure	1239:1269	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	4	53	theme	innate	931:936	arg1	responses					958:966	both innate and adaptive immune responses	926:966	both innate and adaptive immune responses	926:966	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	2	54	theme	pro-repair	444:453	arg1	phenotype					455:463	a pro-repair phenotype	442:463	a pro-repair phenotype	442:463	Here we reveal that CSPGs also play a critical role in preventing inflammation resolution by blocking the conversion of pro-inflammatory immune cells to a pro-repair phenotype in rodent models of SCI.					
35614038	6	55	theme	inflammatory	1318:1329	arg1	pathology					1331:1339	prolonged inflammatory pathology	1308:1339	prolonged inflammatory pathology	1308:1339	These findings establish CSPGs as critical mediators of inflammation resolution failure after SCI in rodents, which leads to prolonged inflammatory pathology and irreversible tissue destruction.					
35614038	4	56	theme	clusters	733:740	arg1	Analysis					685:692	Analysis	685:692	Analysis of phenotypically distinct immune cell clusters	685:740	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	4	57	theme	lymphocyte	837:846	arg1	infiltration					848:859	T lymphocyte infiltration	835:859	T lymphocyte infiltration	835:859	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	4	58	theme	immune	951:956	arg1	responses					958:966	both innate and adaptive immune responses	926:966	both innate and adaptive immune responses	926:966	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	4	59	theme	T	835:835	arg1	infiltration					848:859	T lymphocyte infiltration	835:859	T lymphocyte infiltration	835:859	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	3	60	theme	expression	634:643	arg1	profiles					645:652	pro-inflammatory protein and gene expression profiles	600:652	profiles	645:652	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	5	61	theme	CSPG	996:999	arg1	activation					1001:1010	CSPG activation	996:1010	CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells	996:1069	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	1	62	theme	axonal	223:228	arg1	growth					230:235	growth	230:235	growth	230:235	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	4	63	theme	adaptive	942:949	arg1	responses					958:966	both innate and adaptive immune responses	926:966	both innate and adaptive immune responses	926:966	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	5	64	theme	pro-repair	1047:1056	arg1	cells					1065:1069	pro-repair immune cells	1047:1069	pro-repair immune cells	1047:1069	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	3	65	theme	enzymatic	510:518	arg1	digestion					520:528	enzymatic digestion	510:528	enzymatic digestion of CSPG glycosaminoglycans	510:555	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	1	66	theme	growth	230:235	arg1	inhibitors					209:218	potent inhibitors	202:218	potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI)	202:286	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	1	66	theme	growth	230:235	arg1	proteoglycans					173:185	Chondroitin sulfate proteoglycans	153:185	Chondroitin sulfate proteoglycans (CSPGs)	153:193	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	0	67	theme	inflammation	80:91	arg1	resolution					93:102	inflammation resolution	80:102	inflammation resolution	80:102	Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury.					
35614038	0	68	from	TLR4	108:111	arg1	models					123:128	rodent models	116:128	rodent models of spinal cord injury	116:150	Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury.					
35614038	5	69	theme	immune	1058:1063	arg1	cells					1065:1069	pro-repair immune cells	1047:1069	pro-repair immune cells	1047:1069	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	4	70	theme	distinct	712:719	arg1	clusters					733:740	phenotypically distinct immune cell clusters	697:740	phenotypically distinct immune cell clusters	697:740	Analysis of phenotypically distinct immune cell clusters revealed CSPG-mediated modulation of macrophage and microglial subtypes which, together with T lymphocyte infiltration and composition changes, suggests a role for CSPGs in modulating both innate and adaptive immune responses after SCI.					
35614038	5	71	from	activation	1001:1010	arg1	cells					1065:1069	pro-repair immune cells	1047:1069	pro-repair immune cells	1047:1069	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	2	72	theme	SCI	485:487	arg1	models					475:480	rodent models	468:480	rodent models of SCI	468:487	Here we reveal that CSPGs also play a critical role in preventing inflammation resolution by blocking the conversion of pro-inflammatory immune cells to a pro-repair phenotype in rodent models of SCI.					
35614038	5	73	theme	modulation	1171:1180	arg1	driver					1140:1145	a key driver	1134:1145	a key driver of CSPG-mediated immune modulation	1134:1180	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	5	73	theme	modulation	1171:1180	arg1	signalling					1120:1129	TLR4 signalling	1115:1129	TLR4 signalling	1115:1129	Mechanistically, CSPG activation of a pro-inflammatory phenotype in pro-repair immune cells was found to be TLR4-dependent, identifying TLR4 signalling as a key driver of CSPG-mediated immune modulation.					
35614038	3	74	theme	gene	629:632	arg1	profiles					645:652	pro-inflammatory protein and gene expression profiles	600:652	profiles	645:652	We demonstrate that enzymatic digestion of CSPG glycosaminoglycans enhances immune cell clearance and reduces pro-inflammatory protein and gene expression profiles at key resolution time points.					
35614038	1	75	theme	Chondroitin	153:163	arg1	inhibitors					209:218	potent inhibitors	202:218	potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI)	202:286	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	1	75	theme	Chondroitin	153:163	arg1	CSPGs					188:192	CSPGs	188:192	CSPGs	188:192	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	1	75	theme	Chondroitin	153:163	arg1	proteoglycans					173:185	Chondroitin sulfate proteoglycans	153:185	Chondroitin sulfate proteoglycans (CSPGs)	153:193	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	1	76	theme	neuroplasticity	241:255	arg1	inhibitors					209:218	potent inhibitors	202:218	potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI)	202:286	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35614038	1	76	theme	neuroplasticity	241:255	arg1	proteoglycans					173:185	Chondroitin sulfate proteoglycans	153:185	Chondroitin sulfate proteoglycans (CSPGs)	153:193	Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI).					
35907557	12	0	theme	VEGF	2590:2593	arg1	activity					2595:2602	VEGF activity	2590:2602	VEGF activity	2590:2602	Incorporation of CMPs and HA in the same matrix synergistically enhanced VEGF activity as compared with simpler matrices.					
35907557	9	1	theme	key	1925:1927	arg1	challenges					1929:1938	key challenges	1925:1938	key challenges in growth factor therapy for regenerative medicine	1925:1989	GAHCM has significant potential to overcome key challenges in growth factor therapy for regenerative medicine.					
35907557	8	2	theme	ECM-based	1717:1725	arg1	materials					1727:1735	ECM-based materials	1717:1735	ECM-based materials	1717:1735	Overall, these findings demonstrate the use of ECM-based materials to stimulate efficient gene transfer and regulate cellular phenotype, resulting in improved control of growth factor activity for wound repair.					
35907557	1	3	theme	desired	283:289	arg1	frames					296:301	desired time frames	283:301	desired time frames	283:301	Growth factor therapy has demonstrated great promise for chronic wound repair, but controlling growth factor activity and cell phenotype over desired time frames remains a critical challenge.					
35907557	11	4	theme	hyaluronic	2322:2331	arg1	matrix					2347:2352	a hyaluronic acid-collagen matrix	2320:2352	a hyaluronic acid-collagen matrix	2320:2352	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	3	5	theme	transfer	721:728	arg1	kinetics					730:737	growth factor gene transfer kinetics	702:737	growth factor gene transfer kinetics	702:737	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	13	6	theme	recalcitrant	2780:2791	arg1	wounds					2793:2798	recalcitrant wounds	2780:2798	recalcitrant wounds	2780:2798	Accordingly, GAHCM will advance our ability to leverage growth factor signaling for wound healing, resulting in new long-term treatments for recalcitrant wounds.					
35907557	4	7	theme	%	869:869	arg1	PEI					884:886	50% CMP-modified PEI	867:886	50% CMP-modified PEI (50 CP)	867:894	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	4	7	theme	%	869:869	arg1	CP					892:893	50 CP	889:893	50 CP	889:893	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	9	8	theme	factor	1950:1955	arg1	therapy					1957:1963	growth factor therapy	1943:1963	growth factor therapy for regenerative medicine	1943:1989	GAHCM has significant potential to overcome key challenges in growth factor therapy for regenerative medicine.					
35907557	10	9	theme	SIGNIFICANCE	2005:2016	arg1	STATEMENT					1992:2000	STATEMENT	1992:2000	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.	1992:2266	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	5	10	from	incorporation	1023:1035	arg1	hydrogel					1050:1057	the hydrogel	1046:1057	the hydrogel	1046:1057	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	1	11	theme	wound	206:210	arg1	repair					212:217	chronic wound repair	198:217	chronic wound repair	198:217	Growth factor therapy has demonstrated great promise for chronic wound repair, but controlling growth factor activity and cell phenotype over desired time frames remains a critical challenge.					
35907557	7	12	theme	wounds	1662:1667	arg1	closure					1635:1641	closure	1635:1641	closure of murine splinted wounds	1635:1667	Application of these VEGF-A-50 CP samples stimulated prolonged pro-healing responses, including the TGF-β1-induced myofibroblast-like phenotypes and enhanced closure of murine splinted wounds.					
35907557	3	13	theme	cell	756:759	arg1	behavior					761:768	cell behavior	756:768	cell behavior	756:768	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	6	14	theme	factor-A	1343:1350	arg1	-GAHCM					1360:1365	vascular endothelial growth factor-A (VEGF-A)-GAHCM	1315:1365	vascular endothelial growth factor-A (VEGF-A)-GAHCM	1315:1365	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	13	15	theme	new	2751:2753	arg1	treatments					2765:2774	new long-term treatments	2751:2774	new long-term treatments for recalcitrant wounds	2751:2798	Accordingly, GAHCM will advance our ability to leverage growth factor signaling for wound healing, resulting in new long-term treatments for recalcitrant wounds.					
35907557	7	16	theme	murine	1646:1651	arg1	wounds					1662:1667	murine splinted wounds	1646:1667	murine splinted wounds	1646:1667	Application of these VEGF-A-50 CP samples stimulated prolonged pro-healing responses, including the TGF-β1-induced myofibroblast-like phenotypes and enhanced closure of murine splinted wounds.					
35907557	0	17	from	behavior	90:97	arg1	fibroblasts					102:112	fibroblasts	102:112	fibroblasts	102:112	Modified hyaluronic acid-collagen matrices trigger efficient gene transfer and prohealing behavior in fibroblasts for improved wound repair.					
35907557	10	18	theme	growth	2182:2187	arg1	factor					2189:2194	growth factor	2182:2194	growth factor signaling	2182:2204	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	3	19	theme	growth	793:798	arg1	activity					807:814	enhanced growth factor activity	784:814	enhanced growth factor activity for wound repair	784:831	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	6	20	theme	endothelial	1324:1334	arg1	VEGF-A					1353:1358	VEGF-A	1353:1358	VEGF-A	1353:1358	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	6	20	theme	endothelial	1324:1334	arg1	factor-A					1343:1350	vascular endothelial growth factor-A	1315:1350	vascular endothelial growth factor-A (VEGF-A)-GAHCM	1315:1365	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	5	21	theme	significant	1070:1080	arg1	increase					1082:1089	a significant increase	1068:1089	a significant increase in gene transfection efficiency	1068:1121	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	1	22	theme	controlling	224:234	arg1	activity					250:257	controlling growth factor activity	224:257	controlling growth factor activity	224:257	Growth factor therapy has demonstrated great promise for chronic wound repair, but controlling growth factor activity and cell phenotype over desired time frames remains a critical challenge.					
35907557	11	23	theme	acid-collagen	2333:2345	arg1	matrix					2347:2352	a hyaluronic acid-collagen matrix	2320:2352	a hyaluronic acid-collagen matrix	2320:2352	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	11	24	theme	behavior	2507:2514	arg1	expression					2457:2466	controlled and localized growth factor expression	2418:2466	controlled and localized growth factor expression	2418:2466	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	11	24	theme	behavior	2507:2514	arg1	regulation					2488:2497	matrix-mediated regulation	2472:2497	matrix-mediated regulation of cell behavior	2472:2514	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	5	25	theme	HA	1040:1041	arg1	incorporation					1023:1035	the incorporation	1019:1035	the incorporation of HA in the hydrogel	1019:1057	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	4	26	theme	enhanced	903:910	arg1	retention					912:920	enhanced retention	903:920	enhanced retention of polyplexes in HCM hydrogels	903:951	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	11	27	theme	gene	2302:2305	arg1	delivery					2307:2314	CMP gene delivery	2298:2314	CMP gene delivery	2298:2314	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	3	28	theme	wound	820:824	arg1	repair					826:831	wound repair	820:831	wound repair	820:831	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	1	29	theme	factor	148:153	arg1	therapy					155:161	Growth factor therapy	141:161	Growth factor therapy	141:161	Growth factor therapy has demonstrated great promise for chronic wound repair, but controlling growth factor activity and cell phenotype over desired time frames remains a critical challenge.					
35907557	7	30	theme	myofibroblast-like	1592:1609	arg1	phenotypes					1611:1620	the TGF-β1-induced myofibroblast-like phenotypes	1573:1620	the TGF-β1-induced myofibroblast-like phenotypes	1573:1620	Application of these VEGF-A-50 CP samples stimulated prolonged pro-healing responses, including the TGF-β1-induced myofibroblast-like phenotypes and enhanced closure of murine splinted wounds.					
35907557	11	31	theme	GAHCM	2272:2276	arg1	strategy					2278:2285	Our GAHCM strategy	2268:2285	Our GAHCM strategy	2268:2285	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	9	32	from	challenges	1929:1938	arg1	therapy					1957:1963	growth factor therapy	1943:1963	growth factor therapy for regenerative medicine	1943:1989	GAHCM has significant potential to overcome key challenges in growth factor therapy for regenerative medicine.					
35907557	10	33	theme	existing	2245:2252	arg1	formulations					2254:2265	existing formulations	2245:2265	existing formulations	2245:2265	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	10	34	theme	growth	2045:2050	arg1	therapies					2059:2067	growth factor therapies	2045:2067	growth factor therapies in wound treatment	2045:2086	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	6	35	theme	CP	1375:1376	arg1	matrix					1378:1383	the 50 CP matrix	1368:1383	the 50 CP matrix	1368:1383	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	3	36	theme	tethers	625:631	arg1	number					602:607	the number	598:607	the number of CMP-collagen tethers	598:631	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	3	36	theme	tethers	625:631	arg1	composition					645:655	the ECM composition	637:655	the ECM composition	637:655	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	8	37	theme	improved	1820:1827	arg1	control					1829:1835	improved control	1820:1835	improved control of growth factor activity for wound repair	1820:1878	Overall, these findings demonstrate the use of ECM-based materials to stimulate efficient gene transfer and regulate cellular phenotype, resulting in improved control of growth factor activity for wound repair.					
35907557	4	38	theme	non-CMP	980:986	arg1	polyplexes					997:1006	non-CMP modified polyplexes	980:1006	non-CMP modified polyplexes	980:1006	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	5	39	theme	HA-CD44	1250:1256	arg1	signaling					1258:1266	HA-CD44 signaling	1250:1266	HA-CD44 signaling	1250:1266	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	1	40	theme	great	180:184	arg1	promise					186:192	great promise	180:192	great promise for chronic wound repair	180:217	Growth factor therapy has demonstrated great promise for chronic wound repair, but controlling growth factor activity and cell phenotype over desired time frames remains a critical challenge.					
35907557	11	41	theme	CMP	2298:2300	arg1	delivery					2307:2314	CMP gene delivery	2298:2314	CMP gene delivery	2298:2314	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	11	42	theme	growth	2443:2448	arg1	factor					2450:2455	controlled and localized growth factor	2418:2455	controlled and localized growth factor expression	2418:2466	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	10	43	theme	wound	2072:2076	arg1	treatment					2078:2086	wound treatment	2072:2086	wound treatment	2072:2086	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	5	44	from	increase	1082:1089	arg1	efficiency					1112:1121	gene transfection efficiency	1094:1121	gene transfection efficiency	1094:1121	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	8	45	theme	factor	1847:1852	arg1	activity					1854:1861	growth factor activity	1840:1861	growth factor activity for wound repair	1840:1878	Overall, these findings demonstrate the use of ECM-based materials to stimulate efficient gene transfer and regulate cellular phenotype, resulting in improved control of growth factor activity for wound repair.					
35907557	7	46	theme	pro-healing	1540:1550	arg1	responses					1552:1560	prolonged pro-healing responses	1530:1560	prolonged pro-healing responses	1530:1560	Application of these VEGF-A-50 CP samples stimulated prolonged pro-healing responses, including the TGF-β1-induced myofibroblast-like phenotypes and enhanced closure of murine splinted wounds.					
35907557	7	46	theme	pro-healing	1540:1550	arg1	phenotypes					1611:1620	the TGF-β1-induced myofibroblast-like phenotypes	1573:1620	the TGF-β1-induced myofibroblast-like phenotypes	1573:1620	Application of these VEGF-A-50 CP samples stimulated prolonged pro-healing responses, including the TGF-β1-induced myofibroblast-like phenotypes and enhanced closure of murine splinted wounds.					
35907557	2	47	theme	collagen	525:532	arg1	CMPs					552:555	CMPs	552:555	CMPs	552:555	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	2	47	theme	collagen	525:532	arg1	peptides					542:549	collagen mimetic peptides	525:549	collagen mimetic peptides (CMPs)	525:556	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	0	48	theme	improved	118:125	arg1	repair					133:138	improved wound repair	118:138	improved wound repair	118:138	Modified hyaluronic acid-collagen matrices trigger efficient gene transfer and prohealing behavior in fibroblasts for improved wound repair.					
35907557	5	49	theme	transfection	1099:1110	arg1	efficiency					1112:1121	gene transfection efficiency	1094:1121	gene transfection efficiency	1094:1121	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	11	50	theme	matrix-mediated	2472:2486	arg1	regulation					2488:2497	matrix-mediated regulation	2472:2497	matrix-mediated regulation of cell behavior	2472:2514	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	0	51	theme	Modified	0:7	arg1	matrices					34:41	Modified hyaluronic acid-collagen matrices	0:41	Modified hyaluronic acid-collagen matrices	0:41	Modified hyaluronic acid-collagen matrices trigger efficient gene transfer and prohealing behavior in fibroblasts for improved wound repair.					
35907557	1	52	theme	time	291:294	arg1	frames					296:301	desired time frames	283:301	desired time frames	283:301	Growth factor therapy has demonstrated great promise for chronic wound repair, but controlling growth factor activity and cell phenotype over desired time frames remains a critical challenge.					
35907557	11	53	theme	expression	2457:2466	arg1	combination					2403:2413	the combination	2399:2413	the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior	2399:2514	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	0	54	theme	acid-collagen	20:32	arg1	matrices					34:41	Modified hyaluronic acid-collagen matrices	0:41	Modified hyaluronic acid-collagen matrices	0:41	Modified hyaluronic acid-collagen matrices trigger efficient gene transfer and prohealing behavior in fibroblasts for improved wound repair.					
35907557	5	55	theme	gene	1181:1184	arg1	expression					1186:1195	Gaussia luciferase (GLuc) reporter gene expression	1146:1195	Gaussia luciferase (GLuc) reporter gene expression	1146:1195	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	7	56	theme	samples	1511:1517	arg1	Application					1477:1487	Application	1477:1487	Application of these VEGF-A-50 CP samples	1477:1517	Application of these VEGF-A-50 CP samples stimulated prolonged pro-healing responses, including the TGF-β1-induced myofibroblast-like phenotypes and enhanced closure of murine splinted wounds.					
35907557	12	57	theme	same	2553:2556	arg1	matrix					2558:2563	the same matrix	2549:2563	the same matrix	2549:2563	Incorporation of CMPs and HA in the same matrix synergistically enhanced VEGF activity as compared with simpler matrices.					
35907557	9	58	contain	has	1887:1889	arg1	GAHCM					1881:1885	GAHCM	1881:1885	GAHCM	1881:1885	GAHCM has significant potential to overcome key challenges in growth factor therapy for regenerative medicine.					
35907557	9	58	contain	has	1887:1889	arg2	potential					1903:1911	significant potential	1891:1911	significant potential	1891:1911	GAHCM has significant potential to overcome key challenges in growth factor therapy for regenerative medicine.					
35907557	6	59	theme	VEGF-A	1407:1412	arg1	production					1414:1423	sustained VEGF-A production	1397:1423	sustained VEGF-A production for up to 7 days	1397:1440	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	1	60	theme	critical	313:320	arg1	challenge					322:330	a critical challenge	311:330	a critical challenge	311:330	Growth factor therapy has demonstrated great promise for chronic wound repair, but controlling growth factor activity and cell phenotype over desired time frames remains a critical challenge.					
35907557	2	61	theme	acid	490:493	arg1	hydrogels					509:517	hyaluronic acid (HA)-collagen hydrogels	479:517	hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs)	479:556	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	2	62	theme	hyaluronic	479:488	arg1	HA					496:497	HA	496:497	HA	496:497	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	2	62	theme	hyaluronic	479:488	arg1	acid					490:493	hyaluronic acid	479:493	hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs)	479:556	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	5	63	theme	Gaussia	1146:1152	arg1	gene					1181:1184	Gaussia luciferase (GLuc) reporter gene	1146:1184	Gaussia luciferase (GLuc) reporter gene expression	1146:1195	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	13	64	theme	wound	2723:2727	arg1	healing					2729:2735	wound healing	2723:2735	wound healing	2723:2735	Accordingly, GAHCM will advance our ability to leverage growth factor signaling for wound healing, resulting in new long-term treatments for recalcitrant wounds.					
35907557	0	65	theme	gene	61:64	arg1	transfer					66:73	efficient gene transfer	51:73	efficient gene transfer	51:73	Modified hyaluronic acid-collagen matrices trigger efficient gene transfer and prohealing behavior in fibroblasts for improved wound repair.					
35907557	6	66	from	day	1470:1472	arg1	expression					1456:1465	maximal expression	1448:1465	maximal expression at day 5	1448:1474	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	13	67	theme	growth	2695:2700	arg1	factor					2702:2707	growth factor	2695:2707	growth factor signaling	2695:2717	Accordingly, GAHCM will advance our ability to leverage growth factor signaling for wound healing, resulting in new long-term treatments for recalcitrant wounds.					
35907557	11	68	theme	healing	2378:2384	arg1	efficacy					2386:2393	enhanced wound healing efficacy	2363:2393	enhanced wound healing efficacy	2363:2393	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	1	69	theme	growth	236:241	arg1	activity					250:257	controlling growth factor activity	224:257	controlling growth factor activity	224:257	Growth factor therapy has demonstrated great promise for chronic wound repair, but controlling growth factor activity and cell phenotype over desired time frames remains a critical challenge.					
35907557	10	70	theme	growth	2101:2106	arg1	factor					2108:2113	growth factor	2101:2113	growth factor activity	2101:2122	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	3	71	theme	powerful	673:680	arg1	strategy					682:689	a powerful strategy	671:689	a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair	671:831	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	2	72	theme	hyaluronic	378:387	arg1	GAHCM					411:415	GAHCM	411:415	GAHCM	411:415	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	2	72	theme	hyaluronic	378:387	arg1	matrix					403:408	a gene-activated hyaluronic acid-collagen matrix	361:408	a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs)	361:556	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	3	73	theme	gene	716:719	arg1	kinetics					730:737	growth factor gene transfer kinetics	702:737	growth factor gene transfer kinetics	702:737	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	1	74	theme	cell	263:266	arg1	phenotype					268:276	cell phenotype	263:276	cell phenotype	263:276	Growth factor therapy has demonstrated great promise for chronic wound repair, but controlling growth factor activity and cell phenotype over desired time frames remains a critical challenge.					
35907557	4	75	theme	CMP-modified	871:882	arg1	PEI					884:886	50% CMP-modified PEI	867:886	50% CMP-modified PEI (50 CP)	867:894	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	4	75	theme	CMP-modified	871:882	arg1	CP					892:893	50 CP	889:893	50 CP	889:893	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	3	76	theme	growth	702:707	arg1	kinetics					730:737	growth factor gene transfer kinetics	702:737	growth factor gene transfer kinetics	702:737	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	5	77	theme	gene	1202:1205	arg1	expression					1207:1216	gene expression	1202:1216	gene expression	1202:1216	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	9	78	theme	growth	1943:1948	arg1	therapy					1957:1963	growth factor therapy	1943:1963	growth factor therapy for regenerative medicine	1943:1989	GAHCM has significant potential to overcome key challenges in growth factor therapy for regenerative medicine.					
35907557	8	79	theme	materials	1727:1735	arg1	use					1710:1712	the use	1706:1712	the use of ECM-based materials to stimulate efficient gene transfer and regulate cellular phenotype	1706:1804	Overall, these findings demonstrate the use of ECM-based materials to stimulate efficient gene transfer and regulate cellular phenotype, resulting in improved control of growth factor activity for wound repair.					
35907557	1	80	theme	chronic	198:204	arg1	repair					212:217	chronic wound repair	198:217	chronic wound repair	198:217	Growth factor therapy has demonstrated great promise for chronic wound repair, but controlling growth factor activity and cell phenotype over desired time frames remains a critical challenge.					
35907557	7	81	theme	splinted	1653:1660	arg1	wounds					1662:1667	murine splinted wounds	1646:1667	murine splinted wounds	1646:1667	Application of these VEGF-A-50 CP samples stimulated prolonged pro-healing responses, including the TGF-β1-induced myofibroblast-like phenotypes and enhanced closure of murine splinted wounds.					
35907557	4	82	theme	50	867:868	arg1	%					869:869	%	869:869	%	869:869	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	12	83	theme	HA	2543:2544	arg1	Incorporation					2517:2529	Incorporation	2517:2529	Incorporation of CMPs and HA in the same matrix	2517:2563	Incorporation of CMPs and HA in the same matrix synergistically enhanced VEGF activity as compared with simpler matrices.					
35907557	9	84	theme	regenerative	1969:1980	arg1	medicine					1982:1989	regenerative medicine	1969:1989	regenerative medicine	1969:1989	GAHCM has significant potential to overcome key challenges in growth factor therapy for regenerative medicine.					
35907557	8	85	theme	efficient	1750:1758	arg1	transfer					1765:1772	efficient gene transfer	1750:1772	efficient gene transfer	1750:1772	Overall, these findings demonstrate the use of ECM-based materials to stimulate efficient gene transfer and regulate cellular phenotype, resulting in improved control of growth factor activity for wound repair.					
35907557	6	86	theme	growth	1336:1341	arg1	VEGF-A					1353:1358	VEGF-A	1353:1358	VEGF-A	1353:1358	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	6	86	theme	growth	1336:1341	arg1	factor-A					1343:1350	vascular endothelial growth factor-A	1315:1350	vascular endothelial growth factor-A (VEGF-A)-GAHCM	1315:1365	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	8	87	theme	gene	1760:1763	arg1	transfer					1765:1772	efficient gene transfer	1750:1772	efficient gene transfer	1750:1772	Overall, these findings demonstrate the use of ECM-based materials to stimulate efficient gene transfer and regulate cellular phenotype, resulting in improved control of growth factor activity for wound repair.					
35907557	12	88	theme	simpler	2621:2627	arg1	matrices					2629:2636	simpler matrices	2621:2636	simpler matrices	2621:2636	Incorporation of CMPs and HA in the same matrix synergistically enhanced VEGF activity as compared with simpler matrices.					
35907557	4	89	theme	polyplexes	925:934	arg1	retention					912:920	enhanced retention	903:920	enhanced retention of polyplexes in HCM hydrogels	903:951	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	11	90	theme	cell	2502:2505	arg1	behavior					2507:2514	cell behavior	2502:2514	cell behavior	2502:2514	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	3	91	theme	factor	800:805	arg1	activity					807:814	enhanced growth factor activity	784:814	enhanced growth factor activity for wound repair	784:831	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	6	92	theme	vascular	1315:1322	arg1	VEGF-A					1353:1358	VEGF-A	1353:1358	VEGF-A	1353:1358	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	6	92	theme	vascular	1315:1322	arg1	factor-A					1343:1350	vascular endothelial growth factor-A	1315:1350	vascular endothelial growth factor-A (VEGF-A)-GAHCM	1315:1365	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	4	93	from	retention	912:920	arg1	hydrogels					943:951	HCM hydrogels	939:951	HCM hydrogels	939:951	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	11	94	theme	regulation	2488:2497	arg1	combination					2403:2413	the combination	2399:2413	the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior	2399:2514	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	3	95	theme	enhanced	784:791	arg1	activity					807:814	enhanced growth factor activity	784:814	enhanced growth factor activity for wound repair	784:831	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	8	96	theme	cellular	1787:1794	arg1	phenotype					1796:1804	cellular phenotype	1787:1804	cellular phenotype	1787:1804	Overall, these findings demonstrate the use of ECM-based materials to stimulate efficient gene transfer and regulate cellular phenotype, resulting in improved control of growth factor activity for wound repair.					
35907557	1	97	theme	Growth	141:146	arg1	factor					148:153	Growth factor	141:153	Growth factor therapy	141:161	Growth factor therapy has demonstrated great promise for chronic wound repair, but controlling growth factor activity and cell phenotype over desired time frames remains a critical challenge.					
35907557	7	98	theme	TGF-β1-induced	1577:1590	arg1	phenotypes					1611:1620	the TGF-β1-induced myofibroblast-like phenotypes	1573:1620	the TGF-β1-induced myofibroblast-like phenotypes	1573:1620	Application of these VEGF-A-50 CP samples stimulated prolonged pro-healing responses, including the TGF-β1-induced myofibroblast-like phenotypes and enhanced closure of murine splinted wounds.					
35907557	10	99	theme	formulations	2254:2265	arg1	success					2234:2240	the success	2230:2240	the success of existing formulations	2230:2265	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	10	100	theme	factor	2052:2057	arg1	therapies					2059:2067	growth factor therapies	2045:2067	growth factor therapies in wound treatment	2045:2086	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	0	101	theme	prohealing	79:88	arg1	behavior					90:97	prohealing behavior	79:97	prohealing behavior	79:97	Modified hyaluronic acid-collagen matrices trigger efficient gene transfer and prohealing behavior in fibroblasts for improved wound repair.					
35907557	3	102	theme	CMP-collagen	612:623	arg1	tethers					625:631	CMP-collagen tethers	612:631	CMP-collagen tethers	612:631	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	2	103	theme	-collagen	499:507	arg1	hydrogels					509:517	hyaluronic acid (HA)-collagen hydrogels	479:517	hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs)	479:556	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	4	104	theme	HCM	939:941	arg1	hydrogels					943:951	HCM hydrogels	939:951	HCM hydrogels	939:951	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	10	105	dep	STATEMENT	1992:2000	arg1	continued					2211:2219	continued	2211:2219	have continued to limit the success of existing formulations	2206:2265	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	10	106	dep	promise	2033:2039	arg1	providing					2128:2136	providing	2128:2136	providing a microenvironment for cells that maximizes growth factor signaling	2128:2204	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	10	106	dep	promise	2033:2039	arg1	controlling					2089:2099	controlling	2089:2099	controlling growth factor activity	2089:2122	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	11	107	theme	localized	2433:2441	arg1	factor					2450:2455	controlled and localized growth factor	2418:2455	controlled and localized growth factor expression	2418:2466	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	4	108	with	polyplexes	851:860	arg1	PEI					884:886	50% CMP-modified PEI	867:886	50% CMP-modified PEI (50 CP)	867:894	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	4	108	with	polyplexes	851:860	arg1	CP					892:893	50 CP	889:893	50 CP	889:893	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	7	109	theme	prolonged	1530:1538	arg1	responses					1552:1560	prolonged pro-healing responses	1530:1560	prolonged pro-healing responses	1530:1560	Application of these VEGF-A-50 CP samples stimulated prolonged pro-healing responses, including the TGF-β1-induced myofibroblast-like phenotypes and enhanced closure of murine splinted wounds.					
35907557	7	109	theme	prolonged	1530:1538	arg1	phenotypes					1611:1620	the TGF-β1-induced myofibroblast-like phenotypes	1573:1620	the TGF-β1-induced myofibroblast-like phenotypes	1573:1620	Application of these VEGF-A-50 CP samples stimulated prolonged pro-healing responses, including the TGF-β1-induced myofibroblast-like phenotypes and enhanced closure of murine splinted wounds.					
35907557	2	110	theme	mimetic	534:540	arg1	CMPs					552:555	CMPs	552:555	CMPs	552:555	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	2	110	theme	mimetic	534:540	arg1	peptides					542:549	collagen mimetic peptides	525:549	collagen mimetic peptides (CMPs)	525:556	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	0	111	from	transfer	66:73	arg1	fibroblasts					102:112	fibroblasts	102:112	fibroblasts	102:112	Modified hyaluronic acid-collagen matrices trigger efficient gene transfer and prohealing behavior in fibroblasts for improved wound repair.					
35907557	11	112	theme	controlled	2418:2427	arg1	factor					2450:2455	controlled and localized growth factor	2418:2455	controlled and localized growth factor expression	2418:2466	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	10	113	theme	factor	2189:2194	arg1	signaling					2196:2204	growth factor signaling	2182:2204	growth factor signaling	2182:2204	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	0	114	theme	wound	127:131	arg1	repair					133:138	improved wound repair	118:138	improved wound repair	118:138	Modified hyaluronic acid-collagen matrices trigger efficient gene transfer and prohealing behavior in fibroblasts for improved wound repair.					
35907557	5	115	theme	gene	1094:1097	arg1	efficiency					1112:1121	gene transfection efficiency	1094:1121	gene transfection efficiency	1094:1121	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	8	116	theme	growth	1840:1845	arg1	activity					1854:1861	growth factor activity	1840:1861	growth factor activity for wound repair	1840:1878	Overall, these findings demonstrate the use of ECM-based materials to stimulate efficient gene transfer and regulate cellular phenotype, resulting in improved control of growth factor activity for wound repair.					
35907557	11	117	theme	enhanced	2363:2370	arg1	efficacy					2386:2393	enhanced wound healing efficacy	2363:2393	enhanced wound healing efficacy	2363:2393	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	13	118	theme	factor	2702:2707	arg1	signaling					2709:2717	growth factor signaling	2695:2717	growth factor signaling	2695:2717	Accordingly, GAHCM will advance our ability to leverage growth factor signaling for wound healing, resulting in new long-term treatments for recalcitrant wounds.					
35907557	0	119	theme	hyaluronic	9:18	arg1	matrices					34:41	Modified hyaluronic acid-collagen matrices	0:41	Modified hyaluronic acid-collagen matrices	0:41	Modified hyaluronic acid-collagen matrices trigger efficient gene transfer and prohealing behavior in fibroblasts for improved wound repair.					
35907557	8	120	theme	activity	1854:1861	arg1	control					1829:1835	improved control	1820:1835	improved control of growth factor activity for wound repair	1820:1878	Overall, these findings demonstrate the use of ECM-based materials to stimulate efficient gene transfer and regulate cellular phenotype, resulting in improved control of growth factor activity for wound repair.					
35907557	12	121	from	Incorporation	2517:2529	arg1	matrix					2558:2563	the same matrix	2549:2563	the same matrix	2549:2563	Incorporation of CMPs and HA in the same matrix synergistically enhanced VEGF activity as compared with simpler matrices.					
35907557	11	122	theme	factor	2450:2455	arg1	expression					2457:2466	controlled and localized growth factor expression	2418:2466	controlled and localized growth factor expression	2418:2466	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	2	123	theme	acid-collagen	389:401	arg1	GAHCM					411:415	GAHCM	411:415	GAHCM	411:415	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	2	123	theme	acid-collagen	389:401	arg1	matrix					403:408	a gene-activated hyaluronic acid-collagen matrix	361:408	a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs)	361:556	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	5	124	theme	reporter	1172:1179	arg1	gene					1181:1184	Gaussia luciferase (GLuc) reporter gene	1146:1184	Gaussia luciferase (GLuc) reporter gene expression	1146:1195	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	8	125	theme	wound	1867:1871	arg1	repair					1873:1878	wound repair	1867:1878	wound repair	1867:1878	Overall, these findings demonstrate the use of ECM-based materials to stimulate efficient gene transfer and regulate cellular phenotype, resulting in improved control of growth factor activity for wound repair.					
35907557	12	126	theme	CMPs	2534:2537	arg1	Incorporation					2517:2529	Incorporation	2517:2529	Incorporation of CMPs and HA in the same matrix	2517:2563	Incorporation of CMPs and HA in the same matrix synergistically enhanced VEGF activity as compared with simpler matrices.					
35907557	13	127	theme	long-term	2755:2763	arg1	treatments					2765:2774	new long-term treatments	2751:2774	new long-term treatments for recalcitrant wounds	2751:2798	Accordingly, GAHCM will advance our ability to leverage growth factor signaling for wound healing, resulting in new long-term treatments for recalcitrant wounds.					
35907557	2	128	theme	DNA/polyethylenimine	429:448	arg1	polyplexes					456:465	DNA/polyethylenimine (PEI) polyplexes	429:465	DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs)	429:556	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	0	129	theme	efficient	51:59	arg1	transfer					66:73	efficient gene transfer	51:73	efficient gene transfer	51:73	Modified hyaluronic acid-collagen matrices trigger efficient gene transfer and prohealing behavior in fibroblasts for improved wound repair.					
35907557	5	130	theme	expression	1186:1195	arg1	analysis					1134:1141	analysis	1134:1141	analysis of Gaussia luciferase (GLuc) reporter gene expression	1134:1195	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	10	131	from	therapies	2059:2067	arg1	treatment					2078:2086	wound treatment	2072:2086	wound treatment	2072:2086	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	11	132	theme	wound	2372:2376	arg1	healing					2378:2384	wound healing	2372:2384	enhanced wound healing efficacy	2363:2393	Our GAHCM strategy, combining CMP gene delivery and a hyaluronic acid-collagen matrix, enabled enhanced wound healing efficacy via the combination of controlled and localized growth factor expression and matrix-mediated regulation of cell behavior.					
35907557	4	133	theme	modified	988:995	arg1	polyplexes					997:1006	non-CMP modified polyplexes	980:1006	non-CMP modified polyplexes	980:1006	We observed that polyplexes with 50% CMP-modified PEI (50 CP) showed enhanced retention of polyplexes in HCM hydrogels by 2.7-fold as compared to non-CMP modified polyplexes.					
35907557	3	134	theme	ECM	641:643	arg1	composition					645:655	the ECM composition	637:655	the ECM composition	637:655	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	6	135	theme	sustained	1397:1405	arg1	production					1414:1423	sustained VEGF-A production	1397:1423	sustained VEGF-A production for up to 7 days	1397:1440	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	5	136	theme	luciferase	1154:1163	arg1	gene					1181:1184	Gaussia luciferase (GLuc) reporter gene	1146:1184	Gaussia luciferase (GLuc) reporter gene expression	1146:1195	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	7	137	theme	CP	1508:1509	arg1	samples					1511:1517	these VEGF-A-50 CP samples	1492:1517	these VEGF-A-50 CP samples	1492:1517	Application of these VEGF-A-50 CP samples stimulated prolonged pro-healing responses, including the TGF-β1-induced myofibroblast-like phenotypes and enhanced closure of murine splinted wounds.					
35907557	5	138	theme	GLuc	1166:1169	arg1	gene					1181:1184	Gaussia luciferase (GLuc) reporter gene	1146:1184	Gaussia luciferase (GLuc) reporter gene expression	1146:1195	Moreover, the incorporation of HA in the hydrogel promoted a significant increase in gene transfection efficiency based upon analysis of Gaussia luciferase (GLuc) reporter gene expression, and gene expression could be attenuated by blocking HA-CD44 signaling.					
35907557	6	139	dep	7	1435:1435	arg1	to					1432:1433	to	1432:1433	to	1432:1433	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	10	140	theme	factor	2108:2113	arg1	activity					2115:2122	growth factor activity	2101:2122	growth factor activity	2101:2122	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
35907557	1	141	theme	factor	243:248	arg1	activity					250:257	controlling growth factor activity	224:257	controlling growth factor activity	224:257	Growth factor therapy has demonstrated great promise for chronic wound repair, but controlling growth factor activity and cell phenotype over desired time frames remains a critical challenge.					
35907557	9	142	theme	significant	1891:1901	arg1	potential					1903:1911	significant potential	1891:1911	significant potential	1891:1911	GAHCM has significant potential to overcome key challenges in growth factor therapy for regenerative medicine.					
35907557	3	143	theme	factor	709:714	arg1	kinetics					730:737	growth factor gene transfer kinetics	702:737	growth factor gene transfer kinetics	702:737	We hypothesized that manipulating both the number of CMP-collagen tethers and the ECM composition would provide a powerful strategy to control growth factor gene transfer kinetics while regulating cell behavior, resulting in enhanced growth factor activity for wound repair.					
35907557	2	144	theme	gene-activated	363:376	arg1	GAHCM					411:415	GAHCM	411:415	GAHCM	411:415	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	2	144	theme	gene-activated	363:376	arg1	matrix					403:408	a gene-activated hyaluronic acid-collagen matrix	361:408	a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs)	361:556	In this study, we developed a gene-activated hyaluronic acid-collagen matrix (GAHCM) comprising DNA/polyethylenimine (PEI) polyplexes retained on hyaluronic acid (HA)-collagen hydrogels using collagen mimetic peptides (CMPs).					
35907557	6	145	theme	maximal	1448:1454	arg1	expression					1456:1465	maximal expression	1448:1465	maximal expression at day 5	1448:1474	Furthermore, when fibroblasts were exposed to vascular endothelial growth factor-A (VEGF-A)-GAHCM, the 50 CP matrix facilitated sustained VEGF-A production for up to 7 days, with maximal expression at day 5.					
35907557	10	146	theme	great	2027:2031	arg1	promise					2033:2039	great promise	2027:2039	great promise	2027:2039	STATEMENT OF SIGNIFICANCE: Despite great promise for growth factor therapies in wound treatment, controlling growth factor activity and providing a microenvironment for cells that maximizes growth factor signaling have continued to limit the success of existing formulations.					
36935348	0	0	theme	FXR/TGR5	110:117	arg1	pathway					129:135	the FXR/TGR5 signaling pathway	106:135	the FXR/TGR5 signaling pathway	106:135	Blueberry extract alleviated lipopolysaccharide-induced inflammation responses in mice through activating the FXR/TGR5 signaling pathway and regulating gut microbiota.					
36935348	3	1	from	effects	409:415	arg1	response					459:466	inflammation response	446:466	inflammation response	446:466	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	3	2	theme	bile	501:504	arg1	receptors					511:519	bile acid receptors	501:519	bile acid receptors	501:519	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	5	3	theme	factors	877:883	arg1	level					855:859	the level	851:859	the level of inflammatory factors in serum and tissues	851:904	RESULTS our results showed that compared with LPS group, the addition of BE decreased the level of inflammatory factors in serum and tissues, inhibited TLR4/MyD88 signaling pathway, protected intestinal barrier, and activated FXR/TGR5, which was related to gut microbiota (especially Akkermansia).					
36935348	7	4	theme	bioactive	1369:1377	arg1	substances					1379:1388	bioactive substances	1369:1388	bioactive substances in BE	1369:1394	CONCLUSION BE alleviated inflammation response mainly by activating bile acid receptor expression and regulating the gut microbiota, and this effect may be related to the composition of bioactive substances in BE.					
36935348	3	5	theme	acid	506:509	arg1	receptors					511:519	bile acid receptors	501:519	bile acid receptors	501:519	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	7	6	theme	substances	1379:1388	arg1	composition					1354:1364	the composition	1350:1364	the composition of bioactive substances in BE	1350:1394	CONCLUSION BE alleviated inflammation response mainly by activating bile acid receptor expression and regulating the gut microbiota, and this effect may be related to the composition of bioactive substances in BE.					
36935348	6	7	theme	gut	1167:1169	arg1	microbiota					1171:1180	gut microbiota	1167:1180	gut microbiota	1167:1180	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE may be an important factor in regulating gut microbiota.					
36935348	6	8	theme	cyanidin	1091:1098	arg1	C3G					1115:1117	C3G	1115:1117	C3G	1115:1117	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE may be an important factor in regulating gut microbiota.					
36935348	6	8	theme	cyanidin	1091:1098	arg1	3-O-glucoside					1100:1112	cyanidin 3-O-glucoside	1091:1112	cyanidin 3-O-glucoside	1091:1112	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE may be an important factor in regulating gut microbiota.					
36935348	2	9	theme	gut	321:323	arg1	microbiota					325:334	gut microbiota	321:334	gut microbiota	321:334	Anthocyanins regulate inflammation response in mice and may be related to gut microbiota and bile acid receptors.					
36935348	3	10	dep	extract	430:436	arg1	BE					439:440	BE	439:440	BE	439:440	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	0	11	theme	signaling	119:127	arg1	pathway					129:135	the FXR/TGR5 signaling pathway	106:135	the FXR/TGR5 signaling pathway	106:135	Blueberry extract alleviated lipopolysaccharide-induced inflammation responses in mice through activating the FXR/TGR5 signaling pathway and regulating gut microbiota.					
36935348	4	12	theme	LPS+BE	713:718	arg1	group					677:681	control diet (CON) group	658:681	control diet (CON) group	658:681	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	12	theme	LPS+BE	713:718	arg1	group					758:762	LPS+BE (LPS stimulation, 5% BE intervention) group	713:762	LPS+BE (LPS stimulation, 5% BE intervention) group	713:762	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	3	13	from	receptors	511:519	arg1	mice					524:527	mice	524:527	mice administered with Escherichia coli lipopolysaccharide (LPS)	524:587	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	3	14	theme	Escherichia	547:557	arg1	lipopolysaccharide					564:581	Escherichia coli lipopolysaccharide	547:581	Escherichia coli lipopolysaccharide (LPS)	547:587	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	3	14	theme	Escherichia	547:557	arg1	LPS					584:586	LPS	584:586	LPS	584:586	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	3	15	theme	gut	482:484	arg1	microbiota					486:495	gut microbiota	482:495	gut microbiota	482:495	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	7	16	theme	inflammation	1208:1219	arg1	response					1221:1228	inflammation response	1208:1228	inflammation response	1208:1228	CONCLUSION BE alleviated inflammation response mainly by activating bile acid receptor expression and regulating the gut microbiota, and this effect may be related to the composition of bioactive substances in BE.					
36935348	4	17	theme	diet	666:669	arg1	group					706:710	LPS (LPS stimulation) group	684:710	LPS (LPS stimulation) group	684:710	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	17	theme	diet	666:669	arg1	group					677:681	control diet (CON) group	658:681	control diet (CON) group	658:681	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	17	theme	diet	666:669	arg1	group					758:762	LPS+BE (LPS stimulation, 5% BE intervention) group	713:762	LPS+BE (LPS stimulation, 5% BE intervention) group	713:762	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	18	theme	METHOD	590:595	arg1	mice					612:615	METHOD Thirty male KM mice	590:615	METHOD Thirty male KM mice	590:615	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	0	19	from	responses	69:77	arg1	mice					82:85	mice	82:85	mice	82:85	Blueberry extract alleviated lipopolysaccharide-induced inflammation responses in mice through activating the FXR/TGR5 signaling pathway and regulating gut microbiota.					
36935348	2	20	theme	acid	345:348	arg1	receptors					350:358	bile acid receptors	340:358	bile acid receptors	340:358	Anthocyanins regulate inflammation response in mice and may be related to gut microbiota and bile acid receptors.					
36935348	6	21	theme	important	1136:1144	arg1	factor					1146:1151	an important factor	1133:1151	an important factor	1133:1151	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE may be an important factor in regulating gut microbiota.					
36935348	6	21	theme	important	1136:1144	arg1	component					1074:1082	The active component	1063:1082	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE	1063:1124	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE may be an important factor in regulating gut microbiota.					
36935348	2	22	theme	bile	340:343	arg1	receptors					350:358	bile acid receptors	340:358	bile acid receptors	340:358	Anthocyanins regulate inflammation response in mice and may be related to gut microbiota and bile acid receptors.					
36935348	3	23	theme	extract	430:436	arg1	effects					409:415	the effects	405:415	the effects of blueberry extract (BE) on inflammation response	405:466	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	0	24	theme	Blueberry	0:8	arg1	extract					10:16	Blueberry extract	0:16	Blueberry extract	0:16	Blueberry extract alleviated lipopolysaccharide-induced inflammation responses in mice through activating the FXR/TGR5 signaling pathway and regulating gut microbiota.					
36935348	3	25	from	microbiota	486:495	arg1	mice					524:527	mice	524:527	mice administered with Escherichia coli lipopolysaccharide (LPS)	524:587	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	6	26	theme	active	1067:1072	arg1	factor					1146:1151	an important factor	1133:1151	an important factor	1133:1151	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE may be an important factor in regulating gut microbiota.					
36935348	6	26	theme	active	1067:1072	arg1	component					1074:1082	The active component	1063:1082	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE	1063:1124	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE may be an important factor in regulating gut microbiota.					
36935348	4	27	theme	control	658:664	arg1	group					706:710	LPS (LPS stimulation) group	684:710	LPS (LPS stimulation) group	684:710	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	27	theme	control	658:664	arg1	group					677:681	control diet (CON) group	658:681	control diet (CON) group	658:681	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	27	theme	control	658:664	arg1	group					758:762	LPS+BE (LPS stimulation, 5% BE intervention) group	713:762	LPS+BE (LPS stimulation, 5% BE intervention) group	713:762	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	0	28	theme	lipopolysaccharide-induced	29:54	arg1	responses					69:77	lipopolysaccharide-induced inflammation responses	29:77	lipopolysaccharide-induced inflammation responses in mice	29:85	Blueberry extract alleviated lipopolysaccharide-induced inflammation responses in mice through activating the FXR/TGR5 signaling pathway and regulating gut microbiota.					
36935348	7	29	theme	bile	1251:1254	arg1	expression					1270:1279	bile acid receptor expression	1251:1279	bile acid receptor expression	1251:1279	CONCLUSION BE alleviated inflammation response mainly by activating bile acid receptor expression and regulating the gut microbiota, and this effect may be related to the composition of bioactive substances in BE.					
36935348	7	30	theme	acid	1256:1259	arg1	expression					1270:1279	bile acid receptor expression	1251:1279	bile acid receptor expression	1251:1279	CONCLUSION BE alleviated inflammation response mainly by activating bile acid receptor expression and regulating the gut microbiota, and this effect may be related to the composition of bioactive substances in BE.					
36935348	3	31	theme	coli	559:562	arg1	lipopolysaccharide					564:581	Escherichia coli lipopolysaccharide	547:581	Escherichia coli lipopolysaccharide (LPS)	547:587	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	3	31	theme	coli	559:562	arg1	LPS					584:586	LPS	584:586	LPS	584:586	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	2	32	from	response	282:289	arg1	mice					294:297	mice	294:297	mice	294:297	Anthocyanins regulate inflammation response in mice and may be related to gut microbiota and bile acid receptors.					
36935348	0	33	theme	gut	152:154	arg1	microbiota					156:165	gut microbiota	152:165	gut microbiota	152:165	Blueberry extract alleviated lipopolysaccharide-induced inflammation responses in mice through activating the FXR/TGR5 signaling pathway and regulating gut microbiota.					
36935348	1	34	dep	extract	189:195	arg1	BE					198:199	BE	198:199	BE	198:199	BACKGROUND Blueberry extract (BE) is rich in phenols, especially anthocyanins.					
36935348	6	35	from	component	1074:1082	arg1	BE					1123:1124	BE	1123:1124	BE	1123:1124	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE may be an important factor in regulating gut microbiota.					
36935348	4	36	theme	CON	672:674	arg1	group					706:710	LPS (LPS stimulation) group	684:710	LPS (LPS stimulation) group	684:710	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	36	theme	CON	672:674	arg1	group					677:681	control diet (CON) group	658:681	control diet (CON) group	658:681	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	36	theme	CON	672:674	arg1	group					758:762	LPS+BE (LPS stimulation, 5% BE intervention) group	713:762	LPS+BE (LPS stimulation, 5% BE intervention) group	713:762	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	7	37	from	composition	1354:1364	arg1	BE					1393:1394	BE	1393:1394	BE	1393:1394	CONCLUSION BE alleviated inflammation response mainly by activating bile acid receptor expression and regulating the gut microbiota, and this effect may be related to the composition of bioactive substances in BE.					
36935348	2	38	theme	inflammation	269:280	arg1	response					282:289	inflammation response	269:289	inflammation response in mice	269:297	Anthocyanins regulate inflammation response in mice and may be related to gut microbiota and bile acid receptors.					
36935348	5	39	theme	LPS	811:813	arg1	group					815:819	LPS group	811:819	LPS group	811:819	RESULTS our results showed that compared with LPS group, the addition of BE decreased the level of inflammatory factors in serum and tissues, inhibited TLR4/MyD88 signaling pathway, protected intestinal barrier, and activated FXR/TGR5, which was related to gut microbiota (especially Akkermansia).					
36935348	0	40	theme	inflammation	56:67	arg1	responses					69:77	lipopolysaccharide-induced inflammation responses	29:77	lipopolysaccharide-induced inflammation responses in mice	29:85	Blueberry extract alleviated lipopolysaccharide-induced inflammation responses in mice through activating the FXR/TGR5 signaling pathway and regulating gut microbiota.					
36935348	3	41	theme	blueberry	420:428	arg1	extract					430:436	blueberry extract	420:436	blueberry extract (BE)	420:441	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	3	42	theme	study	384:388	arg1	aim					365:367	The aim	361:367	The aim of the present study	361:388	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	3	43	theme	inflammation	446:457	arg1	response					459:466	inflammation response	446:466	inflammation response	446:466	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36935348	5	44	dep	microbiota	1026:1035	arg1	Akkermansia					1049:1059	Akkermansia	1049:1059	Akkermansia	1049:1059	RESULTS our results showed that compared with LPS group, the addition of BE decreased the level of inflammatory factors in serum and tissues, inhibited TLR4/MyD88 signaling pathway, protected intestinal barrier, and activated FXR/TGR5, which was related to gut microbiota (especially Akkermansia).					
36935348	5	45	theme	gut	1022:1024	arg1	microbiota					1026:1035	gut microbiota	1022:1035	gut microbiota (especially Akkermansia)	1022:1060	RESULTS our results showed that compared with LPS group, the addition of BE decreased the level of inflammatory factors in serum and tissues, inhibited TLR4/MyD88 signaling pathway, protected intestinal barrier, and activated FXR/TGR5, which was related to gut microbiota (especially Akkermansia).					
36935348	4	46	theme	LPS	684:686	arg1	group					706:710	LPS (LPS stimulation) group	684:710	LPS (LPS stimulation) group	684:710	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	46	theme	LPS	684:686	arg1	stimulation					693:703	LPS stimulation	689:703	LPS stimulation	689:703	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	46	theme	LPS	684:686	arg1	group					677:681	control diet (CON) group	658:681	control diet (CON) group	658:681	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	7	47	from	BE	1393:1394	arg1	composition					1354:1364	the composition	1350:1364	the composition of bioactive substances in BE	1350:1394	CONCLUSION BE alleviated inflammation response mainly by activating bile acid receptor expression and regulating the gut microbiota, and this effect may be related to the composition of bioactive substances in BE.					
36935348	5	48	theme	intestinal	957:966	arg1	barrier					968:974	intestinal barrier	957:974	intestinal barrier	957:974	RESULTS our results showed that compared with LPS group, the addition of BE decreased the level of inflammatory factors in serum and tissues, inhibited TLR4/MyD88 signaling pathway, protected intestinal barrier, and activated FXR/TGR5, which was related to gut microbiota (especially Akkermansia).					
36935348	1	49	from	phenols	213:219	arg1	rich					205:208	rich	205:208	rich	205:208	BACKGROUND Blueberry extract (BE) is rich in phenols, especially anthocyanins.					
36935348	6	50	dep	3-O-glucoside	1100:1112	arg1	e.g.					1085:1088	e.g.	1085:1088	e.g.	1085:1088	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE may be an important factor in regulating gut microbiota.					
36935348	5	51	theme	signaling	928:936	arg1	pathway					938:944	TLR4/MyD88 signaling pathway	917:944	TLR4/MyD88 signaling pathway	917:944	RESULTS our results showed that compared with LPS group, the addition of BE decreased the level of inflammatory factors in serum and tissues, inhibited TLR4/MyD88 signaling pathway, protected intestinal barrier, and activated FXR/TGR5, which was related to gut microbiota (especially Akkermansia).					
36935348	4	52	dep	LPS+BE	713:718	arg1	intervention					744:755	intervention	744:755	intervention	744:755	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	7	53	theme	receptor	1261:1268	arg1	expression					1270:1279	bile acid receptor expression	1251:1279	bile acid receptor expression	1251:1279	CONCLUSION BE alleviated inflammation response mainly by activating bile acid receptor expression and regulating the gut microbiota, and this effect may be related to the composition of bioactive substances in BE.					
36935348	7	54	theme	gut	1300:1302	arg1	microbiota					1304:1313	the gut microbiota	1296:1313	the gut microbiota	1296:1313	CONCLUSION BE alleviated inflammation response mainly by activating bile acid receptor expression and regulating the gut microbiota, and this effect may be related to the composition of bioactive substances in BE.					
36935348	4	55	theme	LPS	689:691	arg1	group					706:710	LPS (LPS stimulation) group	684:710	LPS (LPS stimulation) group	684:710	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	55	theme	LPS	689:691	arg1	stimulation					693:703	LPS stimulation	689:703	LPS stimulation	689:703	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	5	56	theme	inflammatory	864:875	arg1	factors					877:883	inflammatory factors	864:883	inflammatory factors	864:883	RESULTS our results showed that compared with LPS group, the addition of BE decreased the level of inflammatory factors in serum and tissues, inhibited TLR4/MyD88 signaling pathway, protected intestinal barrier, and activated FXR/TGR5, which was related to gut microbiota (especially Akkermansia).					
36935348	4	57	theme	KM	609:610	arg1	mice					612:615	METHOD Thirty male KM mice	590:615	METHOD Thirty male KM mice	590:615	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	58	theme	LPS	721:723	arg1	stimulation					725:735	LPS stimulation	721:735	LPS stimulation	721:735	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	7	59	from	substances	1379:1388	arg1	BE					1393:1394	BE	1393:1394	BE	1393:1394	CONCLUSION BE alleviated inflammation response mainly by activating bile acid receptor expression and regulating the gut microbiota, and this effect may be related to the composition of bioactive substances in BE.					
36935348	1	60	from	rich	205:208	arg1	phenols					213:219	phenols	213:219	phenols	213:219	BACKGROUND Blueberry extract (BE) is rich in phenols, especially anthocyanins.					
36935348	1	60	from	rich	205:208	arg1	anthocyanins					233:244	anthocyanins	233:244	anthocyanins	233:244	BACKGROUND Blueberry extract (BE) is rich in phenols, especially anthocyanins.					
36935348	1	61	theme	BACKGROUND	168:177	arg1	extract					189:195	BACKGROUND Blueberry extract	168:195	BACKGROUND Blueberry extract (BE)	168:200	BACKGROUND Blueberry extract (BE) is rich in phenols, especially anthocyanins.					
36935348	5	62	from	level	855:859	arg1	serum					888:892	serum	888:892	serum	888:892	RESULTS our results showed that compared with LPS group, the addition of BE decreased the level of inflammatory factors in serum and tissues, inhibited TLR4/MyD88 signaling pathway, protected intestinal barrier, and activated FXR/TGR5, which was related to gut microbiota (especially Akkermansia).					
36935348	5	62	from	level	855:859	arg1	tissues					898:904	tissues	898:904	tissues	898:904	RESULTS our results showed that compared with LPS group, the addition of BE decreased the level of inflammatory factors in serum and tissues, inhibited TLR4/MyD88 signaling pathway, protected intestinal barrier, and activated FXR/TGR5, which was related to gut microbiota (especially Akkermansia).					
36935348	4	63	theme	male	604:607	arg1	mice					612:615	METHOD Thirty male KM mice	590:615	METHOD Thirty male KM mice	590:615	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	64	dep	intervention	744:755	arg1	stimulation					725:735	LPS stimulation	721:735	LPS stimulation	721:735	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	5	65	dep	RESULTS	765:771	arg1	showed					785:790	showed	785:790	showed that compared with LPS group, the addition of BE decreased the level of inflammatory factors in serum and tissues, inhibited TLR4/MyD88 signaling pathway, protected intestinal barrier, and activated FXR/TGR5, which was related to gut microbiota (especially Akkermansia)	785:1060	RESULTS our results showed that compared with LPS group, the addition of BE decreased the level of inflammatory factors in serum and tissues, inhibited TLR4/MyD88 signaling pathway, protected intestinal barrier, and activated FXR/TGR5, which was related to gut microbiota (especially Akkermansia).					
36935348	1	66	theme	Blueberry	179:187	arg1	extract					189:195	BACKGROUND Blueberry extract	168:195	BACKGROUND Blueberry extract (BE)	168:200	BACKGROUND Blueberry extract (BE) is rich in phenols, especially anthocyanins.					
36935348	4	67	dep	groups	650:655	arg1	group					706:710	LPS (LPS stimulation) group	684:710	LPS (LPS stimulation) group	684:710	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	67	dep	groups	650:655	arg1	group					677:681	control diet (CON) group	658:681	control diet (CON) group	658:681	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	4	67	dep	groups	650:655	arg1	group					758:762	LPS+BE (LPS stimulation, 5% BE intervention) group	713:762	LPS+BE (LPS stimulation, 5% BE intervention) group	713:762	METHOD Thirty male KM mice were randomly divided into three groups: control diet (CON) group, LPS (LPS stimulation) group, LPS+BE (LPS stimulation, 5% BE intervention) group.					
36935348	6	68	dep	component	1074:1082	arg1	C3G					1115:1117	C3G	1115:1117	C3G	1115:1117	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE may be an important factor in regulating gut microbiota.					
36935348	6	68	dep	component	1074:1082	arg1	3-O-glucoside					1100:1112	cyanidin 3-O-glucoside	1091:1112	cyanidin 3-O-glucoside	1091:1112	The active component (e.g., cyanidin 3-O-glucoside, C3G) in BE may be an important factor in regulating gut microbiota.					
36935348	3	69	theme	present	376:382	arg1	study					384:388	the present study	372:388	the present study	372:388	The aim of the present study was to explore the effects of blueberry extract (BE) on inflammation response by regulating gut microbiota and bile acid receptors in mice administered with Escherichia coli lipopolysaccharide (LPS).					
36436617	0	0	theme	xenobiotic	85:94	arg1	dyes					100:103	xenobiotic azo dyes	85:103	xenobiotic azo dyes	85:103	Facile synthesis of biopolymer decorated magnetic coreshells for enhanced removal of xenobiotic azo dyes through experimental modelling.					
36436617	8	1	theme	Thermodynamic	1318:1330	arg1	analysis					1332:1339	Thermodynamic analysis	1318:1339	Thermodynamic analysis	1318:1339	Thermodynamic analysis revealed the adsorption was spontaneous, and endothermic in nature.					
36436617	8	2	from	nature	1401:1406	arg1	spontaneous					1369:1379	spontaneous	1369:1379	spontaneous	1369:1379	Thermodynamic analysis revealed the adsorption was spontaneous, and endothermic in nature.					
36436617	2	3	theme	such	341:344	arg1	dyes					356:359	two such xenobitic dyes	337:359	two such xenobitic dyes	337:359	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	2	4	theme	high	426:429	arg1	prevalence					431:440	high prevalence	426:440	high prevalence	426:440	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	7	5	theme	452.4 mg/g	1292:1301	arg1	value					1272:1276	a maximum qe value	1259:1276	a maximum qe value of 426.97 and 452.4 mg/g	1259:1301	The reaction fitted well for Langmuir isotherm and pseudo-first-order kinetics, with a maximum qe value of 426.97 and 452.4 mg/g, respectively.					
36436617	4	6	theme	iron	807:810	arg1	nZVI					816:819	CS@nZVI	813:819	CS@nZVI	813:819	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	4	6	theme	iron	807:810	arg1	groups					758:763	functional groups	747:763	functional groups	747:763	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	4	6	theme	iron	807:810	arg1	iron					807:810	chitosan-modified nanoscale-zerovalent iron	768:810	chitosan-modified nanoscale-zerovalent iron (CS@nZVI)	768:820	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	4	6	theme	iron	807:810	arg1	morphology					708:717	morphology	708:717	morphology	708:717	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	4	6	theme	iron	807:810	arg1	composition					730:740	elemental composition	720:740	elemental composition	720:740	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	1	7	theme	water	175:179	arg1	bodies					181:186	water bodies	175:186	water bodies	175:186	Since contamination of xenobiotics in water bodies has become a global issue, their removal is gaining ample attention lately.					
36436617	0	8	theme	dyes	100:103	arg1	removal					74:80	enhanced removal	65:80	enhanced removal of xenobiotic azo dyes through experimental modelling	65:134	Facile synthesis of biopolymer decorated magnetic coreshells for enhanced removal of xenobiotic azo dyes through experimental modelling.					
36436617	4	9	theme	@	815:815	arg1	nZVI					816:819	CS@nZVI	813:819	CS@nZVI	813:819	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	4	9	theme	@	815:815	arg1	iron					807:810	chitosan-modified nanoscale-zerovalent iron	768:810	chitosan-modified nanoscale-zerovalent iron (CS@nZVI)	768:820	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	5	10	theme	maximum	947:953	arg1	removal					959:965	maximum dye removal	947:965	maximum dye removal	947:965	RSM-CCD model was utilized to assess the combined effect of five independent variables and determine the best condition for maximum dye removal.					
36436617	7	11	theme	maximum	1261:1267	arg1	value					1272:1276	a maximum qe value	1259:1276	a maximum qe value of 426.97 and 452.4 mg/g	1259:1301	The reaction fitted well for Langmuir isotherm and pseudo-first-order kinetics, with a maximum qe value of 426.97 and 452.4 mg/g, respectively.					
36436617	6	12	theme	time	1024:1027	arg1	min					1037:1039	time (20-40) min	1024:1039	time (20-40) min	1024:1039	Interactions between adsorbent dose (2-4) mg, pH (4-8), time (20-40) min, temperature (35-65) 0C, and initial dye concentration (40-60) mg/L was modeled to study the response, i.e., dye removal percentage.					
36436617	6	13	theme	concentration	1082:1094	arg1	mg/L					1104:1107	initial dye concentration (40-60) mg/L	1070:1107	initial dye concentration (40-60) mg/L	1070:1107	Interactions between adsorbent dose (2-4) mg, pH (4-8), time (20-40) min, temperature (35-65) 0C, and initial dye concentration (40-60) mg/L was modeled to study the response, i.e., dye removal percentage.					
36436617	0	14	theme	azo	96:98	arg1	dyes					100:103	xenobiotic azo dyes	85:103	xenobiotic azo dyes	85:103	Facile synthesis of biopolymer decorated magnetic coreshells for enhanced removal of xenobiotic azo dyes through experimental modelling.					
36436617	7	15	theme	426.97	1281:1286	arg1	value					1272:1276	a maximum qe value	1259:1276	a maximum qe value of 426.97 and 452.4 mg/g	1259:1301	The reaction fitted well for Langmuir isotherm and pseudo-first-order kinetics, with a maximum qe value of 426.97 and 452.4 mg/g, respectively.					
36436617	4	16	theme	CS	813:814	arg1	nZVI					816:819	CS@nZVI	813:819	CS@nZVI	813:819	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	4	16	theme	CS	813:814	arg1	iron					807:810	chitosan-modified nanoscale-zerovalent iron	768:810	chitosan-modified nanoscale-zerovalent iron (CS@nZVI)	768:820	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	3	17	theme	nanoparticle	513:524	arg1	aggregation					526:536	nanoparticle aggregation	513:536	nanoparticle aggregation	513:536	nZVI functionalization prevents nanoparticle aggregation and oxidation, enhancing the removal of BCG and BB with an efficiency of 84.96% and 86.21%, respectively.					
36436617	2	18	theme	wastewater	460:469	arg1	matrices					471:478	wastewater matrices	460:478	wastewater matrices	460:478	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	2	19	theme	present	271:277	arg1	study					279:283	the present study	267:283	the present study	267:283	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	6	20	theme	dose	999:1002	arg1	mg					1010:1011	adsorbent dose (2-4) mg	989:1011	adsorbent dose (2-4) mg	989:1011	Interactions between adsorbent dose (2-4) mg, pH (4-8), time (20-40) min, temperature (35-65) 0C, and initial dye concentration (40-60) mg/L was modeled to study the response, i.e., dye removal percentage.					
36436617	9	21	theme	remarkable	1479:1488	arg1	potential					1490:1498	remarkable potential	1479:1498	remarkable potential for real water samples	1479:1521	Moreover, CS@nZVI could be used up to five cycles of dye removal with remarkable potential for real water samples.					
36436617	5	22	theme	variables	900:908	arg1	effect					873:878	the combined effect	860:878	the combined effect of five independent variables	860:908	RSM-CCD model was utilized to assess the combined effect of five independent variables and determine the best condition for maximum dye removal.					
36436617	2	23	theme	green	374:378	arg1	removal					326:332	removal	326:332	removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices	326:478	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	7	24	theme	Langmuir	1203:1210	arg1	isotherm					1212:1219	Langmuir isotherm	1203:1219	Langmuir isotherm	1203:1219	The reaction fitted well for Langmuir isotherm and pseudo-first-order kinetics, with a maximum qe value of 426.97 and 452.4 mg/g, respectively.					
36436617	4	25	theme	nanoscale-zerovalent	786:805	arg1	nZVI					816:819	CS@nZVI	813:819	CS@nZVI	813:819	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	4	25	theme	nanoscale-zerovalent	786:805	arg1	iron					807:810	chitosan-modified nanoscale-zerovalent iron	768:810	chitosan-modified nanoscale-zerovalent iron (CS@nZVI)	768:820	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	1	26	from	contamination	143:155	arg1	bodies					181:186	water bodies	175:186	water bodies	175:186	Since contamination of xenobiotics in water bodies has become a global issue, their removal is gaining ample attention lately.					
36436617	4	27	dep	morphology	708:717	arg1	the					704:706	the	704:706	the	704:706	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	0	28	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of biopolymer	0:29	Facile synthesis of biopolymer decorated magnetic coreshells for enhanced removal of xenobiotic azo dyes through experimental modelling.					
36436617	4	29	theme	chitosan-modified	768:784	arg1	nZVI					816:819	CS@nZVI	813:819	CS@nZVI	813:819	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	4	29	theme	chitosan-modified	768:784	arg1	iron					807:810	chitosan-modified nanoscale-zerovalent iron	768:810	chitosan-modified nanoscale-zerovalent iron (CS@nZVI)	768:820	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	6	30	theme	removal	1154:1160	arg1	percentage					1162:1171	dye removal percentage	1150:1171	dye removal percentage	1150:1171	Interactions between adsorbent dose (2-4) mg, pH (4-8), time (20-40) min, temperature (35-65) 0C, and initial dye concentration (40-60) mg/L was modeled to study the response, i.e., dye removal percentage.					
36436617	6	31	theme	adsorbent	989:997	arg1	mg					1010:1011	adsorbent dose (2-4) mg	989:1011	adsorbent dose (2-4) mg	989:1011	Interactions between adsorbent dose (2-4) mg, pH (4-8), time (20-40) min, temperature (35-65) 0C, and initial dye concentration (40-60) mg/L was modeled to study the response, i.e., dye removal percentage.					
36436617	0	32	theme	biopolymer	20:29	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of biopolymer	0:29	Facile synthesis of biopolymer decorated magnetic coreshells for enhanced removal of xenobiotic azo dyes through experimental modelling.					
36436617	4	33	theme	elemental	720:728	arg1	composition					730:740	elemental composition	720:740	elemental composition	720:740	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	9	34	theme	real	1504:1507	arg1	samples					1515:1521	real water samples	1504:1521	real water samples	1504:1521	Moreover, CS@nZVI could be used up to five cycles of dye removal with remarkable potential for real water samples.					
36436617	2	35	contain	have	421:424	arg1	green					374:378	green	374:378	green	374:378	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	2	35	contain	have	421:424	arg1	BCG					385:387	BCG	385:387	BCG	385:387	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	2	35	contain	have	421:424	arg2	prevalence					431:440	high prevalence	426:440	high prevalence	426:440	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	2	35	contain	have	421:424	arg1	dyes					356:359	two such xenobitic dyes	337:359	two such xenobitic dyes	337:359	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	5	36	theme	combined	864:871	arg1	effect					873:878	the combined effect	860:878	the combined effect of five independent variables	860:908	RSM-CCD model was utilized to assess the combined effect of five independent variables and determine the best condition for maximum dye removal.					
36436617	2	37	dep	BCG	385:387	arg1	Brilliant					394:402	Brilliant blue	394:407	Brilliant blue (BB)	394:412	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	2	37	dep	BCG	385:387	arg1	BB					410:411	BB	410:411	BB	410:411	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	1	38	theme	global	201:206	arg1	issue					208:212	a global issue	199:212	a global issue	199:212	Since contamination of xenobiotics in water bodies has become a global issue, their removal is gaining ample attention lately.					
36436617	9	39	with	cycles	1452:1457	arg1	potential					1490:1498	remarkable potential	1479:1498	remarkable potential for real water samples	1479:1521	Moreover, CS@nZVI could be used up to five cycles of dye removal with remarkable potential for real water samples.					
36436617	3	40	theme	nZVI	481:484	arg1	functionalization					486:502	nZVI functionalization	481:502	nZVI functionalization	481:502	nZVI functionalization prevents nanoparticle aggregation and oxidation, enhancing the removal of BCG and BB with an efficiency of 84.96% and 86.21%, respectively.					
36436617	5	41	theme	RSM-CCD	823:829	arg1	model					831:835	RSM-CCD model	823:835	RSM-CCD model	823:835	RSM-CCD model was utilized to assess the combined effect of five independent variables and determine the best condition for maximum dye removal.					
36436617	9	42	dep	five	1447:1450	arg1	to					1444:1445	to	1444:1445	to	1444:1445	Moreover, CS@nZVI could be used up to five cycles of dye removal with remarkable potential for real water samples.					
36436617	0	43	theme	magnetic	41:48	arg1	coreshells					50:59	magnetic coreshells	41:59	magnetic coreshells for enhanced removal of xenobiotic azo dyes through experimental modelling	41:134	Facile synthesis of biopolymer decorated magnetic coreshells for enhanced removal of xenobiotic azo dyes through experimental modelling.					
36436617	6	44	theme	dye	1150:1152	arg1	percentage					1162:1171	dye removal percentage	1150:1171	dye removal percentage	1150:1171	Interactions between adsorbent dose (2-4) mg, pH (4-8), time (20-40) min, temperature (35-65) 0C, and initial dye concentration (40-60) mg/L was modeled to study the response, i.e., dye removal percentage.					
36436617	0	45	theme	experimental	113:124	arg1	modelling					126:134	experimental modelling	113:134	experimental modelling	113:134	Facile synthesis of biopolymer decorated magnetic coreshells for enhanced removal of xenobiotic azo dyes through experimental modelling.					
36436617	7	46	theme	qe	1269:1270	arg1	value					1272:1276	a maximum qe value	1259:1276	a maximum qe value of 426.97 and 452.4 mg/g	1259:1301	The reaction fitted well for Langmuir isotherm and pseudo-first-order kinetics, with a maximum qe value of 426.97 and 452.4 mg/g, respectively.					
36436617	2	47	theme	BCG	385:387	arg1	removal					326:332	removal	326:332	removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices	326:478	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	8	48	from	spontaneous	1369:1379	arg1	nature					1401:1406	nature	1401:1406	nature	1401:1406	Thermodynamic analysis revealed the adsorption was spontaneous, and endothermic in nature.					
36436617	2	49	theme	blue	404:407	arg1	Brilliant					394:402	Brilliant blue	394:407	Brilliant blue (BB)	394:412	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	2	49	theme	blue	404:407	arg1	BB					410:411	BB	410:411	BB	410:411	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	6	50	dep	percentage	1162:1171	arg1	i.e.					1144:1147	i.e.	1144:1147	i.e.	1144:1147	Interactions between adsorbent dose (2-4) mg, pH (4-8), time (20-40) min, temperature (35-65) 0C, and initial dye concentration (40-60) mg/L was modeled to study the response, i.e., dye removal percentage.					
36436617	4	51	theme	functional	747:756	arg1	groups					758:763	functional groups	747:763	functional groups	747:763	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	4	51	theme	functional	747:756	arg1	nZVI					816:819	CS@nZVI	813:819	CS@nZVI	813:819	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	4	51	theme	functional	747:756	arg1	iron					807:810	chitosan-modified nanoscale-zerovalent iron	768:810	chitosan-modified nanoscale-zerovalent iron (CS@nZVI)	768:820	XRD, FESEM, EDS, and FTIR have been employed to investigate the morphology, elemental composition, and functional groups of chitosan-modified nanoscale-zerovalent iron (CS@nZVI).					
36436617	6	52	theme	temperature	1042:1052	arg1	0C					1062:1063	temperature (35-65) 0C	1042:1063	temperature (35-65) 0C	1042:1063	Interactions between adsorbent dose (2-4) mg, pH (4-8), time (20-40) min, temperature (35-65) 0C, and initial dye concentration (40-60) mg/L was modeled to study the response, i.e., dye removal percentage.					
36436617	5	53	theme	dye	955:957	arg1	removal					959:965	maximum dye removal	947:965	maximum dye removal	947:965	RSM-CCD model was utilized to assess the combined effect of five independent variables and determine the best condition for maximum dye removal.					
36436617	9	54	theme	removal	1466:1472	arg1	cycles					1452:1457	up to five cycles	1441:1457	up to five cycles of dye removal with remarkable potential for real water samples	1441:1521	Moreover, CS@nZVI could be used up to five cycles of dye removal with remarkable potential for real water samples.					
36436617	9	55	theme	dye	1462:1464	arg1	removal					1466:1472	dye removal	1462:1472	dye removal	1462:1472	Moreover, CS@nZVI could be used up to five cycles of dye removal with remarkable potential for real water samples.					
36436617	6	56	theme	35-65	1055:1059	arg1	0C					1062:1063	temperature (35-65) 0C	1042:1063	temperature (35-65) 0C	1042:1063	Interactions between adsorbent dose (2-4) mg, pH (4-8), time (20-40) min, temperature (35-65) 0C, and initial dye concentration (40-60) mg/L was modeled to study the response, i.e., dye removal percentage.					
36436617	3	57	theme	%	627:627	arg1	efficiency					597:606	an efficiency	594:606	an efficiency of 84.96% and 86.21%, respectively	594:641	nZVI functionalization prevents nanoparticle aggregation and oxidation, enhancing the removal of BCG and BB with an efficiency of 84.96% and 86.21%, respectively.					
36436617	3	58	theme	BB	586:587	arg1	removal					567:573	the removal	563:573	the removal of BCG and BB	563:587	nZVI functionalization prevents nanoparticle aggregation and oxidation, enhancing the removal of BCG and BB with an efficiency of 84.96% and 86.21%, respectively.					
36436617	6	59	theme	dye	1078:1080	arg1	mg/L					1104:1107	initial dye concentration (40-60) mg/L	1070:1107	initial dye concentration (40-60) mg/L	1070:1107	Interactions between adsorbent dose (2-4) mg, pH (4-8), time (20-40) min, temperature (35-65) 0C, and initial dye concentration (40-60) mg/L was modeled to study the response, i.e., dye removal percentage.					
36436617	9	60	theme	CS	1419:1420	arg1	nZVI					1422:1425	CS@nZVI	1419:1425	CS@nZVI	1419:1425	Moreover, CS@nZVI could be used up to five cycles of dye removal with remarkable potential for real water samples.					
36436617	7	61	theme	pseudo-first-order	1225:1242	arg1	kinetics					1244:1251	pseudo-first-order kinetics	1225:1251	pseudo-first-order kinetics	1225:1251	The reaction fitted well for Langmuir isotherm and pseudo-first-order kinetics, with a maximum qe value of 426.97 and 452.4 mg/g, respectively.					
36436617	9	62	used	used	1436:1439	arg2	nZVI					1422:1425	CS@nZVI	1419:1425	CS@nZVI	1419:1425	Moreover, CS@nZVI could be used up to five cycles of dye removal with remarkable potential for real water samples.					
36436617	3	63	theme	%	616:616	arg1	efficiency					597:606	an efficiency	594:606	an efficiency of 84.96% and 86.21%, respectively	594:641	nZVI functionalization prevents nanoparticle aggregation and oxidation, enhancing the removal of BCG and BB with an efficiency of 84.96% and 86.21%, respectively.					
36436617	5	64	used	utilized	841:848	arg2	model					831:835	RSM-CCD model	823:835	RSM-CCD model	823:835	RSM-CCD model was utilized to assess the combined effect of five independent variables and determine the best condition for maximum dye removal.					
36436617	1	65	theme	xenobiotics	160:170	arg1	contamination					143:155	contamination	143:155	contamination of xenobiotics in water bodies	143:186	Since contamination of xenobiotics in water bodies has become a global issue, their removal is gaining ample attention lately.					
36436617	5	66	theme	independent	888:898	arg1	variables					900:908	five independent variables	883:908	five independent variables	883:908	RSM-CCD model was utilized to assess the combined effect of five independent variables and determine the best condition for maximum dye removal.					
36436617	3	67	theme	BCG	578:580	arg1	removal					567:573	the removal	563:573	the removal of BCG and BB	563:587	nZVI functionalization prevents nanoparticle aggregation and oxidation, enhancing the removal of BCG and BB with an efficiency of 84.96% and 86.21%, respectively.					
36436617	6	68	theme	initial	1070:1076	arg1	mg/L					1104:1107	initial dye concentration (40-60) mg/L	1070:1107	initial dye concentration (40-60) mg/L	1070:1107	Interactions between adsorbent dose (2-4) mg, pH (4-8), time (20-40) min, temperature (35-65) 0C, and initial dye concentration (40-60) mg/L was modeled to study the response, i.e., dye removal percentage.					
36436617	2	69	theme	dyes	356:359	arg1	removal					326:332	removal	326:332	removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices	326:478	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	9	70	theme	@	1421:1421	arg1	nZVI					1422:1425	CS@nZVI	1419:1425	CS@nZVI	1419:1425	Moreover, CS@nZVI could be used up to five cycles of dye removal with remarkable potential for real water samples.					
36436617	0	71	theme	enhanced	65:72	arg1	removal					74:80	enhanced removal	65:80	enhanced removal of xenobiotic azo dyes through experimental modelling	65:134	Facile synthesis of biopolymer decorated magnetic coreshells for enhanced removal of xenobiotic azo dyes through experimental modelling.					
36436617	5	72	theme	best	928:931	arg1	condition					933:941	the best condition	924:941	the best condition for maximum dye removal	924:965	RSM-CCD model was utilized to assess the combined effect of five independent variables and determine the best condition for maximum dye removal.					
36436617	2	73	theme	xenobitic	346:354	arg1	dyes					356:359	two such xenobitic dyes	337:359	two such xenobitic dyes	337:359	In the present study, nZVI was synthesized using chitosan for removal of two such xenobitic dyes, Bromocresol green and (BCG) and Brilliant blue (BB), which have high prevalence in freshwater and wastewater matrices.					
36436617	9	74	theme	water	1509:1513	arg1	samples					1515:1521	real water samples	1504:1521	real water samples	1504:1521	Moreover, CS@nZVI could be used up to five cycles of dye removal with remarkable potential for real water samples.					
36436617	1	75	theme	ample	240:244	arg1	attention					246:254	ample attention	240:254	ample attention	240:254	Since contamination of xenobiotics in water bodies has become a global issue, their removal is gaining ample attention lately.					
35114074	5	0	theme	3D	821:822	arg1	cultures					829:836	neural 3D cell cultures	814:836	neural 3D cell cultures	814:836	Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown.					
35114074	5	1	theme	ethylene	868:875	arg1	poly					863:866	poly	863:866	poly(ethylene glycol)	863:883	Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown.					
35114074	5	1	theme	ethylene	868:875	arg1	glycol					877:882	ethylene glycol	868:882	ethylene glycol	868:882	Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown.					
35114074	2	2	theme	advanced	361:368	arg1	models					377:382	advanced tissue models	361:382	advanced tissue models	361:382	LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders.					
35114074	4	3	theme	LN	687:688	arg1	conjugation					690:700	LN conjugation	687:700	LN conjugation	687:700	Engineered hydrogels require elaborate and often cytotoxic chemistries for cross-linking and LN conjugation and provide limited possibilities to tailor the properties of the materials.					
35114074	6	4	theme	high	1066:1069	arg1	viability					1071:1079	high viability	1066:1079	high viability	1066:1079	Encapsulated human neuroblastoma cells demonstrate high viability and grow into spheroids.					
35114074	4	5	dep	provide	706:712	arg1	limited					714:720	limited	714:720	provide limited possibilities to tailor the properties of the materials	706:776	Engineered hydrogels require elaborate and often cytotoxic chemistries for cross-linking and LN conjugation and provide limited possibilities to tailor the properties of the materials.					
35114074	7	6	theme	spontaneous	1190:1200	arg1	differentiation					1202:1216	spontaneous differentiation	1190:1216	spontaneous differentiation to neural fate	1190:1231	Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN.					
35114074	1	7	from	matrix	162:167	arg1	brain					207:211	the developing brain	192:211	the developing brain	192:211	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	5	8	theme	recombinant	937:947	arg1	LN-521					949:954	human recombinant LN-521	931:954	human recombinant LN-521 using bioorthogonal copper-free click chemistry	931:1002	Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown.					
35114074	5	9	theme	modular	786:792	arg1	system					803:808	a modular hydrogel system	784:808	a modular hydrogel system	784:808	Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown.					
35114074	3	10	theme	clinical	572:579	arg1	translation					581:591	clinical translation	572:591	clinical translation	572:591	Biologically derived hydrogels are rich in fragmented LN and are poorly defined concerning composition, which hampers clinical translation.					
35114074	2	11	theme	relevant	293:300	arg1	matrix					302:307	a biologically relevant matrix	278:307	a biologically relevant matrix for the 3D culture of neurons	278:337	LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders.					
35114074	5	12	theme	copper-free	976:986	arg1	chemistry					994:1002	bioorthogonal copper-free click chemistry	962:1002	bioorthogonal copper-free click chemistry	962:1002	Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown.					
35114074	5	13	theme	hydrogel	794:801	arg1	system					803:808	a modular hydrogel system	784:808	a modular hydrogel system	784:808	Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown.					
35114074	8	14	theme	structures	1384:1393	arg1	integrity					1357:1365	the structural integrity	1342:1365	the structural integrity of 3D bioprinted structures	1342:1393	The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.					
35114074	8	15	theme	bioprinted	1373:1382	arg1	structures					1384:1393	3D bioprinted structures	1370:1393	3D bioprinted structures	1370:1393	The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.					
35114074	8	16	theme	lt-NES	1458:1463	arg1	viability					1413:1421	high viability	1408:1421	high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies	1408:1540	The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.					
35114074	5	17	theme	bioorthogonal	962:974	arg1	chemistry					994:1002	bioorthogonal copper-free click chemistry	962:1002	bioorthogonal copper-free click chemistry	962:1002	Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown.					
35114074	1	18	theme	neuronal	172:179	arg1	tissues					181:187	neuronal tissues	172:187	neuronal tissues	172:187	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	6	19	theme	neuroblastoma	1034:1046	arg1	cells					1048:1052	Encapsulated human neuroblastoma cells	1015:1052	Encapsulated human neuroblastoma cells	1015:1052	Encapsulated human neuroblastoma cells demonstrate high viability and grow into spheroids.					
35114074	7	20	theme	neural	1221:1226	arg1	fate					1228:1231	neural fate	1221:1231	neural fate	1221:1231	Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN.					
35114074	1	21	from	components	130:139	arg1	niches					234:239	neural stem cell niches	217:239	neural stem cell niches	217:239	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	1	21	from	components	130:139	arg1	matrix					162:167	the extracellular matrix	144:167	the extracellular matrix of neuronal tissues in the developing brain	144:211	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	4	22	theme	cytotoxic	643:651	arg1	chemistries					653:663	cytotoxic chemistries	643:663	cytotoxic chemistries for cross-linking and LN conjugation	643:700	Engineered hydrogels require elaborate and often cytotoxic chemistries for cross-linking and LN conjugation and provide limited possibilities to tailor the properties of the materials.					
35114074	1	23	theme	tissues	181:187	arg1	niches					234:239	neural stem cell niches	217:239	neural stem cell niches	217:239	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	1	23	theme	tissues	181:187	arg1	matrix					162:167	the extracellular matrix	144:167	the extracellular matrix of neuronal tissues in the developing brain	144:211	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	0	24	theme	Cross-Linked	16:27	arg1	Hydrogels					48:56	Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels	0:56	Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.	0:105	Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.					
35114074	5	25	theme	cell	824:827	arg1	cultures					829:836	neural 3D cell cultures	814:836	neural 3D cell cultures	814:836	Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown.					
35114074	3	26	from	LN	508:509	arg1	rich					489:492	rich	489:492	rich	489:492	Biologically derived hydrogels are rich in fragmented LN and are poorly defined concerning composition, which hampers clinical translation.					
35114074	5	27	theme	click	988:992	arg1	chemistry					994:1002	bioorthogonal copper-free click chemistry	962:1002	bioorthogonal copper-free click chemistry	962:1002	Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown.					
35114074	7	28	theme	neuroepithelial	1116:1130	arg1	cells					1137:1141	Long-term neuroepithelial stem cells	1106:1141	Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels	1106:1176	Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN.					
35114074	7	28	theme	neuroepithelial	1116:1130	arg1	lt-NES					1144:1149	lt-NES	1144:1149	lt-NES	1144:1149	Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN.					
35114074	2	29	theme	cell-based	388:397	arg1	therapies					399:407	cell-based therapies	388:407	cell-based therapies for the treatment of neurological disorders	388:451	LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders.					
35114074	0	30	theme	Bioorthogonally	0:14	arg1	Hydrogels					48:56	Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels	0:56	Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.	0:105	Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.					
35114074	6	31	theme	human	1028:1032	arg1	cells					1048:1052	Encapsulated human neuroblastoma cells	1015:1052	Encapsulated human neuroblastoma cells	1015:1052	Encapsulated human neuroblastoma cells demonstrate high viability and grow into spheroids.					
35114074	3	32	link	derived	467:473	arg1	hydrogels					475:483	Biologically derived hydrogels	454:483	Biologically derived hydrogels	454:483	Biologically derived hydrogels are rich in fragmented LN and are poorly defined concerning composition, which hampers clinical translation.					
35114074	2	33	theme	3D	317:318	arg1	culture					320:326	the 3D culture	313:326	the 3D culture of neurons	313:337	LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders.					
35114074	6	34	theme	Encapsulated	1015:1026	arg1	cells					1048:1052	Encapsulated human neuroblastoma cells	1015:1052	Encapsulated human neuroblastoma cells	1015:1052	Encapsulated human neuroblastoma cells demonstrate high viability and grow into spheroids.					
35114074	5	35	theme	human	931:935	arg1	LN-521					949:954	human recombinant LN-521	931:954	human recombinant LN-521 using bioorthogonal copper-free click chemistry	931:1002	Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown.					
35114074	1	36	theme	developing	196:205	arg1	brain					207:211	the developing brain	192:211	the developing brain	192:211	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	2	37	theme	disorders	443:451	arg1	treatment					417:425	the treatment	413:425	the treatment of neurological disorders	413:451	LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders.					
35114074	8	38	theme	structural	1346:1355	arg1	integrity					1357:1365	the structural integrity	1342:1365	the structural integrity of 3D bioprinted structures	1342:1393	The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.					
35114074	0	39	theme	3D	62:63	arg1	Culture					79:85	3D Neuronal Cell Culture	62:85	3D Neuronal Cell Culture	62:85	Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.					
35114074	4	40	theme	Engineered	594:603	arg1	hydrogels					605:613	Engineered hydrogels	594:613	Engineered hydrogels	594:613	Engineered hydrogels require elaborate and often cytotoxic chemistries for cross-linking and LN conjugation and provide limited possibilities to tailor the properties of the materials.					
35114074	7	41	theme	stem	1132:1135	arg1	cells					1137:1141	Long-term neuroepithelial stem cells	1106:1141	Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels	1106:1176	Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN.					
35114074	7	41	theme	stem	1132:1135	arg1	lt-NES					1144:1149	lt-NES	1144:1149	lt-NES	1144:1149	Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN.					
35114074	7	42	theme	higher	1263:1268	arg1	viability					1270:1278	significantly higher viability	1249:1278	significantly higher viability than cells cultured without LN	1249:1309	Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN.					
35114074	8	43	theme	3D	1370:1371	arg1	structures					1384:1393	3D bioprinted structures	1370:1393	3D bioprinted structures	1370:1393	The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.					
35114074	8	44	theme	high	1408:1411	arg1	viability					1413:1421	high viability	1408:1421	high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies	1408:1540	The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.					
35114074	1	45	theme	neural	217:222	arg1	niches					234:239	neural stem cell niches	217:239	neural stem cell niches	217:239	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	0	46	theme	Cell	74:77	arg1	Culture					79:85	3D Neuronal Cell Culture	62:85	3D Neuronal Cell Culture	62:85	Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.					
35114074	1	47	from	niches	234:239	arg1	brain					207:211	the developing brain	192:211	the developing brain	192:211	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	4	48	dep	require	615:621	arg1	chemistries					653:663	cytotoxic chemistries	643:663	cytotoxic chemistries for cross-linking and LN conjugation	643:700	Engineered hydrogels require elaborate and often cytotoxic chemistries for cross-linking and LN conjugation and provide limited possibilities to tailor the properties of the materials.					
35114074	4	48	dep	require	615:621	arg1	elaborate					623:631	elaborate	623:631	elaborate	623:631	Engineered hydrogels require elaborate and often cytotoxic chemistries for cross-linking and LN conjugation and provide limited possibilities to tailor the properties of the materials.					
35114074	4	49	theme	materials	768:776	arg1	properties					750:759	the properties	746:759	the properties of the materials	746:776	Engineered hydrogels require elaborate and often cytotoxic chemistries for cross-linking and LN conjugation and provide limited possibilities to tailor the properties of the materials.					
35114074	0	50	theme	Neuronal	65:72	arg1	Culture					79:85	3D Neuronal Cell Culture	62:85	3D Neuronal Cell Culture	62:85	Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.					
35114074	3	51	theme	fragmented	497:506	arg1	LN					508:509	fragmented LN	497:509	fragmented LN	497:509	Biologically derived hydrogels are rich in fragmented LN and are poorly defined concerning composition, which hampers clinical translation.					
35114074	3	52	from	rich	489:492	arg1	LN					508:509	fragmented LN	497:509	fragmented LN	497:509	Biologically derived hydrogels are rich in fragmented LN and are poorly defined concerning composition, which hampers clinical translation.					
35114074	1	53	theme	key	126:128	arg1	components					130:139	key components	126:139	key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches	126:239	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	1	53	theme	key	126:128	arg1	Laminins					107:114	Laminins	107:114	Laminins (LNs)	107:120	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	2	54	theme	LN-presenting	242:254	arg1	hydrogels					256:264	LN-presenting hydrogels	242:264	LN-presenting hydrogels	242:264	LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders.					
35114074	8	55	theme	therapies	1532:1540	arg1	development					1506:1516	development	1506:1516	development	1506:1516	The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.					
35114074	8	55	theme	therapies	1532:1540	arg1	biofabrication					1487:1500	biofabrication	1487:1500	biofabrication	1487:1500	The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.					
35114074	2	56	theme	neurons	331:337	arg1	culture					320:326	the 3D culture	313:326	the 3D culture of neurons	313:337	LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders.					
35114074	1	57	theme	stem	224:227	arg1	niches					234:239	neural stem cell niches	217:239	neural stem cell niches	217:239	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	8	58	theme	extruded	1449:1456	arg1	lt-NES					1458:1463	bioprinted and syringe extruded lt-NES	1426:1463	bioprinted and syringe extruded lt-NES	1426:1463	The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.					
35114074	8	59	theme	cell-based	1521:1530	arg1	therapies					1532:1540	cell-based therapies	1521:1540	cell-based therapies	1521:1540	The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.					
35114074	2	60	theme	tissue	370:375	arg1	models					377:382	advanced tissue models	361:382	advanced tissue models	361:382	LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders.					
35114074	8	61	theme	bioprinted	1426:1435	arg1	lt-NES					1458:1463	bioprinted and syringe extruded lt-NES	1426:1463	bioprinted and syringe extruded lt-NES	1426:1463	The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.					
35114074	1	62	theme	cell	229:232	arg1	niches					234:239	neural stem cell niches	217:239	neural stem cell niches	217:239	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35114074	3	63	theme	derived	467:473	arg1	hydrogels					475:483	Biologically derived hydrogels	454:483	Biologically derived hydrogels	454:483	Biologically derived hydrogels are rich in fragmented LN and are poorly defined concerning composition, which hampers clinical translation.					
35114074	7	64	theme	Long-term	1106:1114	arg1	cells					1137:1141	Long-term neuroepithelial stem cells	1106:1141	Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels	1106:1176	Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN.					
35114074	7	64	theme	Long-term	1106:1114	arg1	lt-NES					1144:1149	lt-NES	1144:1149	lt-NES	1144:1149	Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN.					
35114074	2	65	theme	neurological	430:441	arg1	disorders					443:451	neurological disorders	430:451	neurological disorders	430:451	LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders.					
35114074	2	66	theme	models	377:382	arg1	development					346:356	development	346:356	development of advanced tissue models and cell-based therapies for the treatment of neurological disorders	346:451	LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders.					
35114074	2	67	theme	therapies	399:407	arg1	development					346:356	development	346:356	development of advanced tissue models and cell-based therapies for the treatment of neurological disorders	346:451	LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders.					
35114074	5	68	theme	neural	814:819	arg1	cultures					829:836	neural 3D cell cultures	814:836	neural 3D cell cultures	814:836	Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown.					
35114074	0	69	theme	Hyaluronan-Laminin	29:46	arg1	Hydrogels					48:56	Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels	0:56	Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.	0:105	Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.					
35114074	1	70	theme	extracellular	148:160	arg1	matrix					162:167	the extracellular matrix	144:167	the extracellular matrix of neuronal tissues in the developing brain	144:211	Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches.					
35796806	5	0	theme	meat	949:952	arg1	quality					954:960	meat quality	949:960	meat quality	949:960	After that, the feedlot performance, carcass characteristics, meat quality, and economic return were evaluated.					
35796806	3	1	theme	stem	602:605	arg1	starch					607:612	pineapple stem starch	592:612	pineapple stem starch	592:612	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	8	2	theme	stem	1438:1441	arg1	starch					1443:1448	pineapple stem starch	1428:1448	pineapple stem starch	1428:1448	As a substitute for cassava or corn, pineapple stem starch had no negative impact on the feedlot performance, carcass characteristics, and meat quality.					
35796806	1	3	theme	pineapple	215:223	arg1	starch					230:235	The pineapple stem starch	211:235	The pineapple stem starch	211:235	The pineapple stem starch substituted for ground cassava or corn as a carbohydrate source in the concentrate diet.					
35796806	9	4	theme	feedlot	1630:1636	arg1	diets					1645:1649	the feedlot steers diets	1626:1649	the feedlot steers diets	1626:1649	These results indicate that pineapple stem starch could be a useful feedstuff for the feedlot steers diets as an alternative starch source.					
35796806	0	5	theme	carcass	147:153	arg1	characteristics					155:169	carcass characteristics	147:169	carcass characteristics	147:169	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	3	6	theme	different	503:511	arg1	sources					520:526	three different starch sources	497:526	three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design	497:815	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	6	7	theme	fatty	1231:1235	arg1	acids					1237:1241	fatty acids	1231:1241	fatty acids	1231:1241	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	6	8	theme	average	1068:1074	arg1	gain					1082:1085	average daily gain	1068:1085	average daily gain	1068:1085	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	8	9	theme	negative	1457:1464	arg1	impact					1466:1471	no negative impact	1454:1471	no negative impact	1454:1471	As a substitute for cassava or corn, pineapple stem starch had no negative impact on the feedlot performance, carcass characteristics, and meat quality.					
35796806	0	10	theme	or	113:114	arg1	evaluation					199:208	economic evaluation	190:208	economic evaluation	190:208	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	0	10	theme	or	113:114	arg1	344 days					116:123	206 or 344 days	109:123	206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation	109:208	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	0	10	theme	or	113:114	arg1	performance					134:144	feedlot performance	126:144	feedlot performance	126:144	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	0	10	theme	or	113:114	arg1	quality					177:183	meat quality	172:183	meat quality	172:183	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	3	11	theme	concentrate	531:541	arg1	diets					543:547	concentrate diets	531:547	concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication)	531:771	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	9	12	theme	starch	1669:1674	arg1	source					1676:1681	an alternative starch source	1654:1681	an alternative starch source	1654:1681	These results indicate that pineapple stem starch could be a useful feedstuff for the feedlot steers diets as an alternative starch source.					
35796806	3	13	theme	feeding	638:644	arg1	period					659:664	(1) period 1	655:666	(1) period 1 for 206 days	655:679	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	13	theme	feeding	638:644	arg1	period					688:693	(2) period 2	684:695	(2) period 2	684:695	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	13	theme	feeding	638:644	arg1	periods					646:652	two different feeding periods	624:652	two different feeding periods: (1) period 1 for 206 days or (2) period 2	624:695	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	0	14	theme	cattle	90:95	arg1	feedlot					97:103	a cattle feedlot	88:103	a cattle feedlot	88:103	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	8	15	theme	feedlot	1480:1486	arg1	performance					1488:1498	the feedlot performance	1476:1498	the feedlot performance	1476:1498	As a substitute for cassava or corn, pineapple stem starch had no negative impact on the feedlot performance, carcass characteristics, and meat quality.					
35796806	6	16	theme	weight	1055:1060	arg1	gain					1062:1065	a greater weight gain	1045:1065	a greater weight gain	1045:1065	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	7	17	theme	economic	1374:1381	arg1	return					1383:1388	the greatest economic return	1361:1388	the greatest economic return	1361:1388	The steers fed PS showed the greatest economic return.					
35796806	6	18	theme	gain	1104:1107	arg1	ratio					1109:1113	lower feed: gain ratio	1092:1113	lower feed: gain ratio	1092:1113	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	6	19	theme	feed	1098:1101	arg1	ratio					1109:1113	lower feed: gain ratio	1092:1113	lower feed: gain ratio	1092:1113	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	3	20	dep	period	688:693	arg1	2					685:685	2	685:685	2	685:685	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	2	21	used	used	341:344	arg2	experiment					330:339	The experiment	326:339	The experiment	326:339	The experiment used 36 Holstein crossbred steers (aged 22 months) with an average initial body weight of 453.0 ± 35.3 kg.					
35796806	8	22	dep	a	1394:1394	arg1	substitute					1396:1405	substitute	1396:1405	substitute for cassava or corn	1396:1425	As a substitute for cassava or corn, pineapple stem starch had no negative impact on the feedlot performance, carcass characteristics, and meat quality.					
35796806	2	23	theme	453.0 ± 35.3 kg	431:445	arg1	weight					421:426	an average initial body weight	397:426	an average initial body weight of 453.0 ± 35.3 kg	397:445	The experiment used 36 Holstein crossbred steers (aged 22 months) with an average initial body weight of 453.0 ± 35.3 kg.					
35796806	0	24	theme	economic	190:197	arg1	evaluation					199:208	economic evaluation	190:208	economic evaluation	190:208	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	0	24	theme	economic	190:197	arg1	344 days					116:123	206 or 344 days	109:123	206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation	109:208	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	5	25	theme	carcass	924:930	arg1	characteristics					932:946	carcass characteristics	924:946	carcass characteristics	924:946	After that, the feedlot performance, carcass characteristics, meat quality, and economic return were evaluated.					
35796806	3	26	with	corn	557:560	arg1	period					659:664	(1) period 1	655:666	(1) period 1 for 206 days	655:679	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	26	with	corn	557:560	arg1	period					688:693	(2) period 2	684:695	(2) period 2	684:695	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	26	with	corn	557:560	arg1	periods					646:652	two different feeding periods	624:652	two different feeding periods: (1) period 1 for 206 days or (2) period 2	624:695	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	27	dep	period	659:664	arg1	1					656:656	1	656:656	1	656:656	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	0	28	theme	corn	56:59	arg1	grain					61:65	corn grain	56:65	corn grain	56:65	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	6	29	theme	meat	1213:1216	arg1	quality					1218:1224	meat quality	1213:1224	meat quality	1213:1224	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	6	30	theme	daily	1076:1080	arg1	gain					1082:1085	average daily gain	1068:1085	average daily gain	1068:1085	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	4	31	theme	periods	878:884	arg1	end					854:856	the end	850:856	the end of the experimental periods	850:884	The animals were slaughtered at the end of the experimental periods.					
35796806	2	32	theme	aged	376:379	arg1	steers					368:373	36 Holstein crossbred steers	346:373	36 Holstein crossbred steers (aged 22 months)	346:390	The experiment used 36 Holstein crossbred steers (aged 22 months) with an average initial body weight of 453.0 ± 35.3 kg.					
35796806	2	32	theme	aged	376:379	arg1	22 months					381:389	aged 22 months	376:389	aged 22 months	376:389	The experiment used 36 Holstein crossbred steers (aged 22 months) with an average initial body weight of 453.0 ± 35.3 kg.					
35796806	0	33	theme	pineapple	14:22	arg1	starch					29:34	pineapple stem starch	14:34	pineapple stem starch	14:34	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	1	34	theme	ground	253:258	arg1	cassava					260:266	ground cassava	253:266	ground cassava	253:266	The pineapple stem starch substituted for ground cassava or corn as a carbohydrate source in the concentrate diet.					
35796806	0	35	theme	starch	29:34	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.	0:209	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	2	36	theme	body	416:419	arg1	weight					421:426	an average initial body weight	397:426	an average initial body weight of 453.0 ± 35.3 kg	397:445	The experiment used 36 Holstein crossbred steers (aged 22 months) with an average initial body weight of 453.0 ± 35.3 kg.					
35796806	0	37	dep	a	39:39	arg1	ground					70:75	ground	70:75	ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation	70:208	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	0	37	dep	a	39:39	arg1	substitute					41:50	substitute	41:50	substitute for corn grain	41:65	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	3	38	theme	randomized	799:808	arg1	design					810:815	a completely randomized design	786:815	a completely randomized design	786:815	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	2	39	theme	initial	408:414	arg1	weight					421:426	an average initial body weight	397:426	an average initial body weight of 453.0 ± 35.3 kg	397:445	The experiment used 36 Holstein crossbred steers (aged 22 months) with an average initial body weight of 453.0 ± 35.3 kg.					
35796806	1	40	from	source	294:299	arg1	diet					320:323	the concentrate diet	304:323	the concentrate diet	304:323	The pineapple stem starch substituted for ground cassava or corn as a carbohydrate source in the concentrate diet.					
35796806	1	41	theme	carbohydrate	281:292	arg1	source					294:299	a carbohydrate source	279:299	a carbohydrate source in the concentrate diet	279:323	The pineapple stem starch substituted for ground cassava or corn as a carbohydrate source in the concentrate diet.					
35796806	3	42	theme	pineapple	592:600	arg1	starch					607:612	pineapple stem starch	592:612	pineapple stem starch	592:612	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	9	43	theme	stem	1582:1585	arg1	feedstuff					1612:1620	a useful feedstuff	1603:1620	a useful feedstuff for the feedlot steers diets	1603:1649	These results indicate that pineapple stem starch could be a useful feedstuff for the feedlot steers diets as an alternative starch source.					
35796806	9	43	theme	stem	1582:1585	arg1	starch					1587:1592	pineapple stem starch	1572:1592	pineapple stem starch	1572:1592	These results indicate that pineapple stem starch could be a useful feedstuff for the feedlot steers diets as an alternative starch source.					
35796806	6	44	theme	lower	1092:1096	arg1	ratio					1109:1113	lower feed: gain ratio	1092:1113	lower feed: gain ratio	1092:1113	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	3	45	theme	experimental	452:463	arg1	units					465:469	The experimental units	448:469	The experimental units	448:469	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	46	with	starch	607:612	arg1	period					659:664	(1) period 1	655:666	(1) period 1 for 206 days	655:679	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	46	with	starch	607:612	arg1	period					688:693	(2) period 2	684:695	(2) period 2	684:695	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	46	with	starch	607:612	arg1	periods					646:652	two different feeding periods	624:652	two different feeding periods: (1) period 1 for 206 days or (2) period 2	624:695	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	8	47	theme	pineapple	1428:1436	arg1	starch					1443:1448	pineapple stem starch	1428:1448	pineapple stem starch	1428:1448	As a substitute for cassava or corn, pineapple stem starch had no negative impact on the feedlot performance, carcass characteristics, and meat quality.					
35796806	1	48	theme	stem	225:228	arg1	starch					230:235	The pineapple stem starch	211:235	The pineapple stem starch	211:235	The pineapple stem starch substituted for ground cassava or corn as a carbohydrate source in the concentrate diet.					
35796806	9	49	theme	steers	1638:1643	arg1	diets					1645:1649	the feedlot steers diets	1626:1649	the feedlot steers diets	1626:1649	These results indicate that pineapple stem starch could be a useful feedstuff for the feedlot steers diets as an alternative starch source.					
35796806	3	50	theme	different	628:636	arg1	period					659:664	(1) period 1	655:666	(1) period 1 for 206 days	655:679	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	50	theme	different	628:636	arg1	period					688:693	(2) period 2	684:695	(2) period 2	684:695	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	50	theme	different	628:636	arg1	periods					646:652	two different feeding periods	624:652	two different feeding periods: (1) period 1 for 206 days or (2) period 2	624:695	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	0	51	theme	meat	172:175	arg1	344 days					116:123	206 or 344 days	109:123	206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation	109:208	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	0	51	theme	meat	172:175	arg1	quality					177:183	meat quality	172:183	meat quality	172:183	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	6	52	theme	feeding	1304:1310	arg1	periods					1293:1299	both periods	1288:1299	both periods of feeding	1288:1310	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	3	53	dep	diets	543:547	arg1	steers					749:754	two steers	745:754	concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication)	531:771	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	53	dep	diets	543:547	arg1	replicates					719:728	replicates	719:728	replicates per treatment	719:742	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	6	54	theme	matter	1173:1178	arg1	intake					1180:1185	dry matter intake	1169:1185	dry matter intake	1169:1185	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	9	55	theme	alternative	1657:1667	arg1	source					1676:1681	an alternative starch source	1654:1681	an alternative starch source	1654:1681	These results indicate that pineapple stem starch could be a useful feedstuff for the feedlot steers diets as an alternative starch source.					
35796806	3	56	theme	starch	513:518	arg1	sources					520:526	three different starch sources	497:526	three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design	497:815	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	57	with	cassava	575:581	arg1	period					659:664	(1) period 1	655:666	(1) period 1 for 206 days	655:679	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	57	with	cassava	575:581	arg1	period					688:693	(2) period 2	684:695	(2) period 2	684:695	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	57	with	cassava	575:581	arg1	periods					646:652	two different feeding periods	624:652	two different feeding periods: (1) period 1 for 206 days or (2) period 2	624:695	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	5	58	theme	feedlot	903:909	arg1	performance					911:921	the feedlot performance	899:921	the feedlot performance	899:921	After that, the feedlot performance, carcass characteristics, meat quality, and economic return were evaluated.					
35796806	3	59	theme	ground	550:555	arg1	GC					563:564	GC	563:564	GC	563:564	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	59	theme	ground	550:555	arg1	corn					557:560	ground corn	550:560	ground corn (GC)	550:565	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	59	theme	ground	550:555	arg1	PS					615:616	PS	615:616	PS	615:616	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	7	60	theme	greatest	1365:1372	arg1	return					1383:1388	the greatest economic return	1361:1388	the greatest economic return	1361:1388	The steers fed PS showed the greatest economic return.					
35796806	6	61	theme	greater	1047:1053	arg1	gain					1062:1065	a greater weight gain	1045:1065	a greater weight gain	1045:1065	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	3	62	theme	diets	543:547	arg1	sources					520:526	three different starch sources	497:526	three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design	497:815	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	0	63	theme	feedlot	126:132	arg1	344 days					116:123	206 or 344 days	109:123	206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation	109:208	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	0	63	theme	feedlot	126:132	arg1	performance					134:144	feedlot performance	126:144	feedlot performance	126:144	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	8	64	theme	carcass	1501:1507	arg1	characteristics					1509:1523	carcass characteristics	1501:1523	carcass characteristics	1501:1523	As a substitute for cassava or corn, pineapple stem starch had no negative impact on the feedlot performance, carcass characteristics, and meat quality.					
35796806	0	65	dep	344 days	116:123	arg1	evaluation					199:208	economic evaluation	190:208	economic evaluation	190:208	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	0	65	dep	344 days	116:123	arg1	344 days					116:123	206 or 344 days	109:123	206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation	109:208	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	0	65	dep	344 days	116:123	arg1	performance					134:144	feedlot performance	126:144	feedlot performance	126:144	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	0	65	dep	344 days	116:123	arg1	characteristics					155:169	carcass characteristics	147:169	carcass characteristics	147:169	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	0	65	dep	344 days	116:123	arg1	quality					177:183	meat quality	172:183	meat quality	172:183	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	0	66	from	cassava	77:83	arg1	feedlot					97:103	a cattle feedlot	88:103	a cattle feedlot	88:103	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	6	67	theme	dry	1169:1171	arg1	intake					1180:1185	dry matter intake	1169:1185	dry matter intake	1169:1185	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	3	68	dep	periods	646:652	arg1	period					659:664	(1) period 1	655:666	(1) period 1 for 206 days	655:679	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	68	dep	periods	646:652	arg1	period					688:693	(2) period 2	684:695	(2) period 2	684:695	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	68	dep	periods	646:652	arg1	periods					646:652	two different feeding periods	624:652	two different feeding periods: (1) period 1 for 206 days or (2) period 2	624:695	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	8	69	theme	meat	1530:1533	arg1	quality					1535:1541	meat quality	1530:1541	meat quality	1530:1541	As a substitute for cassava or corn, pineapple stem starch had no negative impact on the feedlot performance, carcass characteristics, and meat quality.					
35796806	1	70	theme	concentrate	308:318	arg1	diet					320:323	the concentrate diet	304:323	the concentrate diet	304:323	The pineapple stem starch substituted for ground cassava or corn as a carbohydrate source in the concentrate diet.					
35796806	5	71	theme	economic	967:974	arg1	return					976:981	economic return	967:981	economic return	967:981	After that, the feedlot performance, carcass characteristics, meat quality, and economic return were evaluated.					
35796806	3	72	with	344 days	701:708	arg1	six					715:717	six	715:717	six	715:717	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	8	73	contain	had	1450:1452	arg1	starch					1443:1448	pineapple stem starch	1428:1448	pineapple stem starch	1428:1448	As a substitute for cassava or corn, pineapple stem starch had no negative impact on the feedlot performance, carcass characteristics, and meat quality.					
35796806	8	73	contain	had	1450:1452	arg2	impact					1466:1471	no negative impact	1454:1471	no negative impact	1454:1471	As a substitute for cassava or corn, pineapple stem starch had no negative impact on the feedlot performance, carcass characteristics, and meat quality.					
35796806	6	74	from	treatments	1274:1283	arg1	periods					1293:1299	both periods	1288:1299	both periods of feeding	1288:1310	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	6	75	contain	had	1041:1043	arg2	gain					1062:1065	a greater weight gain	1045:1065	a greater weight gain	1045:1065	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	6	75	contain	had	1041:1043	arg2	ratio					1109:1113	lower feed: gain ratio	1092:1113	lower feed: gain ratio	1092:1113	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	6	75	contain	had	1041:1043	arg1	steers					1027:1032	the steers	1023:1032	the steers fed PS	1023:1039	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	6	75	contain	had	1041:1043	arg2	gain					1082:1085	average daily gain	1068:1085	average daily gain	1068:1085	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	0	76	theme	stem	24:27	arg1	starch					29:34	pineapple stem starch	14:34	pineapple stem starch	14:34	Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.					
35796806	6	77	dep	intake	1180:1185	arg1	profile					1243:1249	profile	1243:1249	profile	1243:1249	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	4	78	theme	experimental	865:876	arg1	periods					878:884	the experimental periods	861:884	the experimental periods	861:884	The animals were slaughtered at the end of the experimental periods.					
35796806	6	79	theme	carcass	1188:1194	arg1	characteristics					1196:1210	carcass characteristics	1188:1210	carcass characteristics	1188:1210	The results showed that the steers fed PS had a greater weight gain, average daily gain, and lower feed: gain ratio when fed for 206 days than when fed for 344 days, but dry matter intake, carcass characteristics, meat quality, and fatty acids profile did not differ between treatments in both periods of feeding except C14:1 and C18:0.					
35796806	9	80	theme	pineapple	1572:1580	arg1	feedstuff					1612:1620	a useful feedstuff	1603:1620	a useful feedstuff for the feedlot steers diets	1603:1649	These results indicate that pineapple stem starch could be a useful feedstuff for the feedlot steers diets as an alternative starch source.					
35796806	9	80	theme	pineapple	1572:1580	arg1	starch					1587:1592	pineapple stem starch	1572:1592	pineapple stem starch	1572:1592	These results indicate that pineapple stem starch could be a useful feedstuff for the feedlot steers diets as an alternative starch source.					
35796806	3	81	theme	ground	568:573	arg1	CA					584:585	CA	584:585	CA	584:585	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	3	81	theme	ground	568:573	arg1	cassava					575:581	ground cassava	568:581	ground cassava (CA)	568:586	The experimental units were randomly assigned to three different starch sources of concentrate diets: ground corn (GC), ground cassava (CA), or pineapple stem starch (PS) with two different feeding periods: (1) period 1 for 206 days or (2) period 2 for 344 days with six replicates per treatment (two steers per replication), arranged in a completely randomized design.					
35796806	2	82	dep	crossbred	358:366	arg1	Holstein					349:356	36 Holstein	346:356	36 Holstein	346:356	The experiment used 36 Holstein crossbred steers (aged 22 months) with an average initial body weight of 453.0 ± 35.3 kg.					
35796806	2	83	theme	crossbred	358:366	arg1	steers					368:373	36 Holstein crossbred steers	346:373	36 Holstein crossbred steers (aged 22 months)	346:390	The experiment used 36 Holstein crossbred steers (aged 22 months) with an average initial body weight of 453.0 ± 35.3 kg.					
35796806	2	83	theme	crossbred	358:366	arg1	22 months					381:389	aged 22 months	376:389	aged 22 months	376:389	The experiment used 36 Holstein crossbred steers (aged 22 months) with an average initial body weight of 453.0 ± 35.3 kg.					
35796806	2	84	theme	average	400:406	arg1	weight					421:426	an average initial body weight	397:426	an average initial body weight of 453.0 ± 35.3 kg	397:445	The experiment used 36 Holstein crossbred steers (aged 22 months) with an average initial body weight of 453.0 ± 35.3 kg.					
35796806	9	85	theme	useful	1605:1610	arg1	feedstuff					1612:1620	a useful feedstuff	1603:1620	a useful feedstuff for the feedlot steers diets	1603:1649	These results indicate that pineapple stem starch could be a useful feedstuff for the feedlot steers diets as an alternative starch source.					
35796806	9	85	theme	useful	1605:1610	arg1	starch					1587:1592	pineapple stem starch	1572:1592	pineapple stem starch	1572:1592	These results indicate that pineapple stem starch could be a useful feedstuff for the feedlot steers diets as an alternative starch source.					
35871266	3	0	theme	association	294:304	arg1	study					306:310	a genome-wide association study	280:310	a genome-wide association study	280:310	Here, using an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles, we identify a gene, DROUGHT1 (DROT1), encoding a COBRA-like protein that confers drought resistance in rice.					
35871266	5	1	theme	cellulose	759:767	arg1	crystallinity					769:781	cellulose crystallinity	759:781	cellulose crystallinity	759:781	DROT1 improves drought resistance by adjusting cell wall structure by increasing cellulose content and maintaining cellulose crystallinity.					
35871266	7	2	theme	O.	987:988	arg1	rufipogon					990:998	O. rufipogon	987:998	O. rufipogon	987:998	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	7	2	theme	O.	987:988	arg1	rice					981:984	wild rice	976:984	wild rice (O. rufipogon)	976:999	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	7	3	theme	potential	907:915	arg1	haplotype					923:931	The potential elite haplotype	903:931	The potential elite haplotype of DROT1 in upland rice	903:955	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	7	3	theme	potential	907:915	arg1	beneficial					1012:1021	beneficial	1012:1021	beneficial	1012:1021	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	5	4	theme	cell	691:694	arg1	structure					701:709	cell wall structure	691:709	cell wall structure	691:709	DROT1 improves drought resistance by adjusting cell wall structure by increasing cellulose content and maintaining cellulose crystallinity.					
35871266	3	5	from	resistance	467:476	arg1	rice					481:484	rice	481:484	rice	481:484	Here, using an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles, we identify a gene, DROUGHT1 (DROT1), encoding a COBRA-like protein that confers drought resistance in rice.					
35871266	3	6	theme	lines	343:347	arg1	analyses					317:324	analyses	317:324	analyses of introgression lines and transcriptomic profiles	317:375	Here, using an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles, we identify a gene, DROUGHT1 (DROT1), encoding a COBRA-like protein that confers drought resistance in rice.					
35871266	5	7	theme	cellulose	725:733	arg1	content					735:741	cellulose content	725:741	cellulose content	725:741	DROT1 improves drought resistance by adjusting cell wall structure by increasing cellulose content and maintaining cellulose crystallinity.					
35871266	1	8	theme	drought	153:159	arg1	stress					161:166	drought stress	153:166	drought stress	153:166	Upland rice is a distinct ecotype that grows in aerobic environments and tolerates drought stress.					
35871266	3	9	theme	introgression	329:341	arg1	lines					343:347	introgression lines	329:347	introgression lines	329:347	Here, using an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles, we identify a gene, DROUGHT1 (DROT1), encoding a COBRA-like protein that confers drought resistance in rice.					
35871266	6	10	theme	single-nucleotide	793:809	arg1	variation					811:819	A C-to-T single-nucleotide variation	784:819	A C-to-T single-nucleotide variation in the promoter	784:835	A C-to-T single-nucleotide variation in the promoter increases DROT1 expression and drought resistance in upland rice.					
35871266	1	11	theme	Upland	70:75	arg1	ecotype					96:102	a distinct ecotype	85:102	a distinct ecotype that grows in aerobic environments and tolerates drought stress	85:166	Upland rice is a distinct ecotype that grows in aerobic environments and tolerates drought stress.					
35871266	1	11	theme	Upland	70:75	arg1	rice					77:80	Upland rice	70:80	Upland rice	70:80	Upland rice is a distinct ecotype that grows in aerobic environments and tolerates drought stress.					
35871266	4	12	theme	transcription	621:633	arg1	factors					635:641	drought-responsive transcription factors	602:641	drought-responsive transcription factors	602:641	DROT1 is specifically expressed in vascular bundles and is directly repressed by ERF3 and activated by ERF71, both drought-responsive transcription factors.					
35871266	6	13	from	resistance	876:885	arg1	rice					897:900	upland rice	890:900	upland rice	890:900	A C-to-T single-nucleotide variation in the promoter increases DROT1 expression and drought resistance in upland rice.					
35871266	3	14	theme	transcriptomic	353:366	arg1	profiles					368:375	transcriptomic profiles	353:375	transcriptomic profiles	353:375	Here, using an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles, we identify a gene, DROUGHT1 (DROT1), encoding a COBRA-like protein that confers drought resistance in rice.					
35871266	7	15	theme	rice	1043:1046	arg1	varieties					1048:1056	upland rice varieties	1036:1056	upland rice varieties	1036:1056	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	6	16	theme	C-to-T	786:791	arg1	variation					811:819	A C-to-T single-nucleotide variation	784:819	A C-to-T single-nucleotide variation in the promoter	784:835	A C-to-T single-nucleotide variation in the promoter increases DROT1 expression and drought resistance in upland rice.					
35871266	5	17	theme	drought	659:665	arg1	resistance					667:676	drought resistance	659:676	drought resistance	659:676	DROT1 improves drought resistance by adjusting cell wall structure by increasing cellulose content and maintaining cellulose crystallinity.					
35871266	0	18	theme	Natural	0:6	arg1	variation					8:16	Natural variation	0:16	Natural variation of DROT1	0:25	Natural variation of DROT1 confers drought adaptation in upland rice.					
35871266	4	19	theme	vascular	522:529	arg1	bundles					531:537	vascular bundles	522:537	vascular bundles	522:537	DROT1 is specifically expressed in vascular bundles and is directly repressed by ERF3 and activated by ERF71, both drought-responsive transcription factors.					
35871266	7	20	from	haplotype	923:931	arg1	rice					952:955	upland rice	945:955	upland rice	945:955	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	0	21	theme	DROT1	21:25	arg1	variation					8:16	Natural variation	0:16	Natural variation of DROT1	0:25	Natural variation of DROT1 confers drought adaptation in upland rice.					
35871266	7	22	theme	upland	945:950	arg1	rice					952:955	upland rice	945:955	upland rice	945:955	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	7	23	theme	wild	976:979	arg1	rufipogon					990:998	O. rufipogon	987:998	O. rufipogon	987:998	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	7	23	theme	wild	976:979	arg1	rice					981:984	wild rice	976:984	wild rice (O. rufipogon)	976:999	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	3	24	theme	profiles	368:375	arg1	analyses					317:324	analyses	317:324	analyses of introgression lines and transcriptomic profiles	317:375	Here, using an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles, we identify a gene, DROUGHT1 (DROT1), encoding a COBRA-like protein that confers drought resistance in rice.					
35871266	3	25	theme	integrative	249:259	arg1	approach					261:268	an integrative approach	246:268	an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles	246:375	Here, using an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles, we identify a gene, DROUGHT1 (DROT1), encoding a COBRA-like protein that confers drought resistance in rice.					
35871266	6	26	theme	upland	890:895	arg1	rice					897:900	upland rice	890:900	upland rice	890:900	A C-to-T single-nucleotide variation in the promoter increases DROT1 expression and drought resistance in upland rice.					
35871266	1	27	theme	distinct	87:94	arg1	ecotype					96:102	a distinct ecotype	85:102	a distinct ecotype that grows in aerobic environments and tolerates drought stress	85:166	Upland rice is a distinct ecotype that grows in aerobic environments and tolerates drought stress.					
35871266	1	27	theme	distinct	87:94	arg1	rice					77:80	Upland rice	70:80	Upland rice	70:80	Upland rice is a distinct ecotype that grows in aerobic environments and tolerates drought stress.					
35871266	3	28	theme	COBRA-like	427:436	arg1	protein					438:444	a COBRA-like protein	425:444	a COBRA-like protein that confers drought resistance in rice	425:484	Here, using an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles, we identify a gene, DROUGHT1 (DROT1), encoding a COBRA-like protein that confers drought resistance in rice.					
35871266	0	29	theme	drought	35:41	arg1	adaptation					43:52	drought adaptation	35:52	drought adaptation	35:52	Natural variation of DROT1 confers drought adaptation in upland rice.					
35871266	5	30	theme	wall	696:699	arg1	structure					701:709	cell wall structure	691:709	cell wall structure	691:709	DROT1 improves drought resistance by adjusting cell wall structure by increasing cellulose content and maintaining cellulose crystallinity.					
35871266	2	31	theme	resistance	211:220	arg1	unclear					225:231	unclear	225:231	unclear	225:231	However, the genetic basis of its drought resistance is unclear.					
35871266	2	31	theme	resistance	211:220	arg1	basis					190:194	the genetic basis	178:194	the genetic basis of its drought resistance	178:220	However, the genetic basis of its drought resistance is unclear.					
35871266	4	32	theme	drought-responsive	602:619	arg1	factors					635:641	drought-responsive transcription factors	602:641	drought-responsive transcription factors	602:641	DROT1 is specifically expressed in vascular bundles and is directly repressed by ERF3 and activated by ERF71, both drought-responsive transcription factors.					
35871266	2	33	theme	drought	203:209	arg1	resistance					211:220	its drought resistance	199:220	its drought resistance	199:220	However, the genetic basis of its drought resistance is unclear.					
35871266	7	34	theme	upland	1036:1041	arg1	varieties					1048:1056	upland rice varieties	1036:1056	upland rice varieties	1036:1056	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	6	35	theme	drought	868:874	arg1	resistance					876:885	drought resistance	868:885	drought resistance	868:885	A C-to-T single-nucleotide variation in the promoter increases DROT1 expression and drought resistance in upland rice.					
35871266	6	36	from	expression	853:862	arg1	rice					897:900	upland rice	890:900	upland rice	890:900	A C-to-T single-nucleotide variation in the promoter increases DROT1 expression and drought resistance in upland rice.					
35871266	3	37	theme	genome-wide	282:292	arg1	study					306:310	a genome-wide association study	280:310	a genome-wide association study	280:310	Here, using an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles, we identify a gene, DROUGHT1 (DROT1), encoding a COBRA-like protein that confers drought resistance in rice.					
35871266	4	38	dep	ERF71	590:594	arg1	both					597:600	both	597:600	both	597:600	DROT1 is specifically expressed in vascular bundles and is directly repressed by ERF3 and activated by ERF71, both drought-responsive transcription factors.					
35871266	3	39	theme	drought	459:465	arg1	resistance					467:476	drought resistance	459:476	drought resistance in rice	459:484	Here, using an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles, we identify a gene, DROUGHT1 (DROT1), encoding a COBRA-like protein that confers drought resistance in rice.					
35871266	2	40	theme	genetic	182:188	arg1	unclear					225:231	unclear	225:231	unclear	225:231	However, the genetic basis of its drought resistance is unclear.					
35871266	2	40	theme	genetic	182:188	arg1	basis					190:194	the genetic basis	178:194	the genetic basis of its drought resistance	178:220	However, the genetic basis of its drought resistance is unclear.					
35871266	7	41	theme	DROT1	936:940	arg1	haplotype					923:931	The potential elite haplotype	903:931	The potential elite haplotype of DROT1 in upland rice	903:955	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	7	41	theme	DROT1	936:940	arg1	beneficial					1012:1021	beneficial	1012:1021	beneficial	1012:1021	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	6	42	theme	DROT1	847:851	arg1	expression					853:862	DROT1 expression	847:862	DROT1 expression	847:862	A C-to-T single-nucleotide variation in the promoter increases DROT1 expression and drought resistance in upland rice.					
35871266	7	43	theme	elite	917:921	arg1	haplotype					923:931	The potential elite haplotype	903:931	The potential elite haplotype of DROT1 in upland rice	903:955	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	7	43	theme	elite	917:921	arg1	beneficial					1012:1021	beneficial	1012:1021	beneficial	1012:1021	The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.					
35871266	1	44	theme	aerobic	118:124	arg1	environments					126:137	aerobic environments	118:137	aerobic environments	118:137	Upland rice is a distinct ecotype that grows in aerobic environments and tolerates drought stress.					
35871266	6	45	from	variation	811:819	arg1	promoter					828:835	the promoter	824:835	the promoter	824:835	A C-to-T single-nucleotide variation in the promoter increases DROT1 expression and drought resistance in upland rice.					
35871266	0	46	theme	upland	57:62	arg1	rice					64:67	upland rice	57:67	upland rice	57:67	Natural variation of DROT1 confers drought adaptation in upland rice.					
34423918	10	0	theme	K/L	1278:1280	arg1	grade					1282:1286	higher mean K/L grade	1266:1286	higher mean K/L grade	1266:1286	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	7	1	theme	multiplex	795:803	arg1	analysis					814:821	multiplex cytokine analysis	795:821	multiplex cytokine analysis	795:821	Blood samples were used for multiplex cytokine analysis and measures of lipopolysaccharide (LPS) and LPS binding protein.					
34423918	6	2	theme	taxa	725:728	arg1	abundances					739:748	taxa relative abundances	725:748	taxa relative abundances	725:748	Alpha- and beta-diversity and differences in taxa relative abundances were determined.					
34423918	7	3	used	used	786:789	arg2	samples					773:779	Blood samples	767:779	Blood samples	767:779	Blood samples were used for multiplex cytokine analysis and measures of lipopolysaccharide (LPS) and LPS binding protein.					
34423918	13	4	theme	OA	1668:1669	arg1	severity					1671:1678	OA severity	1668:1678	OA severity	1668:1678	Mice transplanted with patient or control microbiota exhibited a significant difference in α-diversity (P = 0.02) and β-diversity, but no differences in OA severity were observed.					
34423918	8	5	theme	patient-	922:929	arg1	samples					955:961	patient- or control-pooled fecal samples	922:961	patient- or control-pooled fecal samples	922:961	Germ-free mice were gavaged with patient- or control-pooled fecal samples and fed a 40% fat, high-sucrose diet for 40 weeks.					
34423918	14	6	theme	serum	1767:1771	arg1	levels					1777:1782	increased serum LPS levels	1757:1782	increased serum LPS levels	1757:1782	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	12	7	theme	LPS	1476:1478	arg1	levels					1433:1438	higher plasma levels	1419:1438	higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001)	1419:1491	Patients had higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001) compared to controls.					
34423918	8	8	theme	control-pooled	934:947	arg1	samples					955:961	patient- or control-pooled fecal samples	922:961	patient- or control-pooled fecal samples	922:961	Germ-free mice were gavaged with patient- or control-pooled fecal samples and fed a 40% fat, high-sucrose diet for 40 weeks.					
34423918	4	9	theme	0-1	563:565	arg1	OA					541:542	no hand OA	533:542	no hand OA	533:542	Controls (n = 42) had no hand OA and a K/L grade of 0-1 for the knees.					
34423918	4	9	theme	0-1	563:565	arg1	grade					554:558	a K/L grade	548:558	a K/L grade of 0-1 for the knees	548:579	Controls (n = 42) had no hand OA and a K/L grade of 0-1 for the knees.					
34423918	10	10	theme	mean	1273:1276	arg1	grade					1282:1286	higher mean K/L grade	1266:1286	higher mean K/L grade	1266:1286	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	10	11	theme	more	1136:1139	arg1	women					1141:1145	more women	1136:1145	more women	1136:1145	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	5	12	theme	stool	608:612	arg1	samples					614:620	stool samples	608:620	stool samples	608:620	Compositional analysis of stool samples was carried out by 16S ribosomal RNA amplicon sequencing.					
34423918	10	13	contain	had	1152:1154	arg2	higher					1158:1163	higher	1158:1163	higher	1158:1163	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	10	13	contain	had	1152:1154	arg1	patients					1075:1082	OA patients	1072:1082	OA patients	1072:1082	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	10	14	theme	Western	1187:1193	arg1	Ontario					1195:1201	mean Western Ontario	1182:1201	mean Western Ontario	1182:1201	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	14	15	from	lack	1710:1713	arg1	microbiota					1741:1750	the gut microbiota	1733:1750	the gut microbiota	1733:1750	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	11	16	theme	level	1357:1361	arg1	composition					1363:1373	genus level composition	1351:1373	genus level composition	1351:1373	There were no significant differences in α- or β-diversity or genus level composition between patients and controls.					
34423918	0	17	theme	Microbial	59:67	arg1	Dysbiosis					69:77	Microbial Dysbiosis	59:77	Microbial Dysbiosis	59:77	Association of Increased Serum Lipopolysaccharide, But Not Microbial Dysbiosis, With Obesity-Related Osteoarthritis.					
34423918	12	18	theme	osteopontin	1443:1453	arg1	levels					1433:1438	higher plasma levels	1419:1438	higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001)	1419:1491	Patients had higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001) compared to controls.					
34423918	1	19	theme	gut	166:168	arg1	dysbiosis					182:190	dysbiosis	182:190	dysbiosis	182:190	OBJECTIVE To test the hypothesis that an altered gut microbiota (dysbiosis) plays a role in obesity-associated osteoarthritis (OA).					
34423918	1	19	theme	gut	166:168	arg1	microbiota					170:179	an altered gut microbiota	155:179	an altered gut microbiota (dysbiosis)	155:191	OBJECTIVE To test the hypothesis that an altered gut microbiota (dysbiosis) plays a role in obesity-associated osteoarthritis (OA).					
34423918	10	20	theme	higher	1266:1271	arg1	grade					1282:1286	higher mean K/L grade	1266:1286	higher mean K/L grade	1266:1286	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	0	21	theme	Obesity-Related	85:99	arg1	Osteoarthritis					101:114	Obesity-Related Osteoarthritis	85:114	Obesity-Related Osteoarthritis	85:114	Association of Increased Serum Lipopolysaccharide, But Not Microbial Dysbiosis, With Obesity-Related Osteoarthritis.					
34423918	7	22	theme	binding	872:878	arg1	protein					880:886	LPS binding protein	868:886	LPS binding protein	868:886	Blood samples were used for multiplex cytokine analysis and measures of lipopolysaccharide (LPS) and LPS binding protein.					
34423918	12	23	theme	higher	1419:1424	arg1	levels					1433:1438	higher plasma levels	1419:1438	higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001)	1419:1491	Patients had higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001) compared to controls.					
34423918	3	24	contain	had	438:440	arg1	n = 50					430:435	n = 50	430:435	n = 50	430:435	OA patients (n = 50) had hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty).					
34423918	3	24	contain	had	438:440	arg2	OA					456:457	hand and knee OA	442:457	hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty)	442:508	OA patients (n = 50) had hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty).					
34423918	3	24	contain	had	438:440	arg1	patients					420:427	OA patients	417:427	OA patients (n = 50)	417:436	OA patients (n = 50) had hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty).					
34423918	5	25	theme	RNA	655:657	arg1	sequencing					668:677	ribosomal RNA amplicon sequencing	645:677	ribosomal RNA amplicon sequencing	645:677	Compositional analysis of stool samples was carried out by 16S ribosomal RNA amplicon sequencing.					
34423918	8	26	theme	40	973:974	arg1	%					975:975	%	975:975	%	975:975	Germ-free mice were gavaged with patient- or control-pooled fecal samples and fed a 40% fat, high-sucrose diet for 40 weeks.					
34423918	14	27	theme	LPS	1883:1885	arg1	absorption					1869:1878	greater absorption	1861:1878	greater absorption of LPS, rather than a dysbiotic microbiota,	1861:1922	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	0	28	theme	Dysbiosis	69:77	arg1	Association					0:10	Association	0:10	Association of Increased Serum Lipopolysaccharide, But Not Microbial Dysbiosis, With Obesity-Related Osteoarthritis	0:114	Association of Increased Serum Lipopolysaccharide, But Not Microbial Dysbiosis, With Obesity-Related Osteoarthritis.					
34423918	11	29	theme	significant	1303:1313	arg1	differences					1315:1325	no significant differences	1300:1325	no significant differences in α- or β-diversity or genus level composition between patients and controls	1300:1403	There were no significant differences in α- or β-diversity or genus level composition between patients and controls.					
34423918	9	30	theme	Knee	1014:1017	arg1	OA					1019:1020	Knee OA	1014:1020	Knee OA	1014:1020	Knee OA was evaluated histologically.					
34423918	2	31	theme	County	386:391	arg1	Project					408:414	the Johnston County Osteoarthritis Project	373:414	the Johnston County Osteoarthritis Project	373:414	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	0	32	theme	Increased	15:23	arg1	Lipopolysaccharide					31:48	Increased Serum Lipopolysaccharide	15:48	Increased Serum Lipopolysaccharide	15:48	Association of Increased Serum Lipopolysaccharide, But Not Microbial Dysbiosis, With Obesity-Related Osteoarthritis.					
34423918	8	33	theme	high-sucrose	982:993	arg1	fat					977:979	a 40% fat	971:979	a 40% fat	971:979	Germ-free mice were gavaged with patient- or control-pooled fecal samples and fed a 40% fat, high-sucrose diet for 40 weeks.					
34423918	8	33	theme	high-sucrose	982:993	arg1	diet					995:998	high-sucrose diet	982:998	high-sucrose diet for 40 weeks	982:1011	Germ-free mice were gavaged with patient- or control-pooled fecal samples and fed a 40% fat, high-sucrose diet for 40 weeks.					
34423918	14	34	dep	CONCLUSION	1695:1704	arg1	suggest					1785:1791	suggest	1785:1791	suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity	1785:1986	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	10	35	theme	higher	1175:1180	arg1	score					1255:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	0	36	theme	Lipopolysaccharide	31:48	arg1	Association					0:10	Association	0:10	Association of Increased Serum Lipopolysaccharide, But Not Microbial Dysbiosis, With Obesity-Related Osteoarthritis	0:114	Association of Increased Serum Lipopolysaccharide, But Not Microbial Dysbiosis, With Obesity-Related Osteoarthritis.					
34423918	10	37	theme	OA	1072:1073	arg1	patients					1075:1082	OA patients	1072:1082	OA patients	1072:1082	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	13	38	from	difference	1592:1601	arg1	P = 0.02					1619:1626	P = 0.02	1619:1626	P = 0.02	1619:1626	Mice transplanted with patient or control microbiota exhibited a significant difference in α-diversity (P = 0.02) and β-diversity, but no differences in OA severity were observed.					
34423918	13	38	from	difference	1592:1601	arg1	β-diversity					1633:1643	β-diversity	1633:1643	β-diversity	1633:1643	Mice transplanted with patient or control microbiota exhibited a significant difference in α-diversity (P = 0.02) and β-diversity, but no differences in OA severity were observed.					
34423918	13	38	from	difference	1592:1601	arg1	α-diversity					1606:1616	α-diversity	1606:1616	α-diversity (P = 0.02)	1606:1627	Mice transplanted with patient or control microbiota exhibited a significant difference in α-diversity (P = 0.02) and β-diversity, but no differences in OA severity were observed.					
34423918	10	39	theme	Universities	1216:1227	arg1	score					1255:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	2	40	theme	mass	329:332	arg1	BMI					341:343	BMI	341:343	BMI	341:343	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	2	40	theme	mass	329:332	arg1	index					334:338	a body mass index	322:338	a body mass index (BMI) ≥30 kg/m2	322:354	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	14	41	theme	increased	1814:1822	arg1	permeability					1835:1846	increased intestinal permeability	1814:1846	increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota,	1814:1922	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	14	42	theme	dysbiotic	1902:1910	arg1	microbiota					1912:1921	a dysbiotic microbiota	1900:1921	a dysbiotic microbiota	1900:1921	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	0	43	with	Association	0:10	arg1	Osteoarthritis					101:114	Obesity-Related Osteoarthritis	85:114	Obesity-Related Osteoarthritis	85:114	Association of Increased Serum Lipopolysaccharide, But Not Microbial Dysbiosis, With Obesity-Related Osteoarthritis.					
34423918	14	44	from	differences	1718:1728	arg1	microbiota					1741:1750	the gut microbiota	1733:1750	the gut microbiota	1733:1750	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	1	45	theme	obesity-associated	209:226	arg1	OA					244:245	OA	244:245	OA	244:245	OBJECTIVE To test the hypothesis that an altered gut microbiota (dysbiosis) plays a role in obesity-associated osteoarthritis (OA).					
34423918	1	45	theme	obesity-associated	209:226	arg1	osteoarthritis					228:241	obesity-associated osteoarthritis	209:241	obesity-associated osteoarthritis (OA)	209:246	OBJECTIVE To test the hypothesis that an altered gut microbiota (dysbiosis) plays a role in obesity-associated osteoarthritis (OA).					
34423918	4	46	theme	hand	536:539	arg1	OA					541:542	no hand OA	533:542	no hand OA	533:542	Controls (n = 42) had no hand OA and a K/L grade of 0-1 for the knees.					
34423918	3	47	theme	Kellgren/Lawrence	460:476	arg1	[K/L					478:481	Kellgren/Lawrence [K/L] grade ≥2	460:491	Kellgren/Lawrence [K/L] grade ≥2	460:491	OA patients (n = 50) had hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty).					
34423918	8	48	theme	Germ-free	889:897	arg1	mice					899:902	Germ-free mice	889:902	Germ-free mice	889:902	Germ-free mice were gavaged with patient- or control-pooled fecal samples and fed a 40% fat, high-sucrose diet for 40 weeks.					
34423918	10	49	theme	Index	1244:1248	arg1	score					1255:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	14	50	theme	differences	1718:1728	arg1	lack					1710:1713	The lack	1706:1713	The lack of differences in the gut microbiota	1706:1750	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	6	51	theme	relative	730:737	arg1	abundances					739:748	taxa relative abundances	725:748	taxa relative abundances	725:748	Alpha- and beta-diversity and differences in taxa relative abundances were determined.					
34423918	7	52	theme	cytokine	805:812	arg1	analysis					814:821	multiplex cytokine analysis	795:821	multiplex cytokine analysis	795:821	Blood samples were used for multiplex cytokine analysis and measures of lipopolysaccharide (LPS) and LPS binding protein.					
34423918	12	53	contain	had	1415:1417	arg1	Patients					1406:1413	Patients	1406:1413	Patients	1406:1413	Patients had higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001) compared to controls.					
34423918	12	53	contain	had	1415:1417	arg2	levels					1433:1438	higher plasma levels	1419:1438	higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001)	1419:1491	Patients had higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001) compared to controls.					
34423918	2	54	theme	blood	267:271	arg1	samples					273:279	blood samples	267:279	blood samples	267:279	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	10	55	dep	RESULTS	1052:1058	arg1	older					1098:1102	older	1098:1102	older	1098:1102	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	10	55	dep	RESULTS	1052:1058	arg1	had					1152:1154	had	1152:1154	had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade	1152:1286	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	14	56	theme	LPS	1773:1775	arg1	levels					1777:1782	increased serum LPS levels	1757:1782	increased serum LPS levels	1757:1782	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	8	57	theme	fecal	949:953	arg1	samples					955:961	patient- or control-pooled fecal samples	922:961	patient- or control-pooled fecal samples	922:961	Germ-free mice were gavaged with patient- or control-pooled fecal samples and fed a 40% fat, high-sucrose diet for 40 weeks.					
34423918	2	58	dep	METHODS	249:255	arg1	samples					273:279	blood samples	267:279	blood samples	267:279	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	2	58	dep	METHODS	249:255	arg1	Stool					257:261	Stool	257:261	Stool	257:261	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	5	59	theme	samples	614:620	arg1	analysis					596:603	Compositional analysis	582:603	Compositional analysis of stool samples	582:620	Compositional analysis of stool samples was carried out by 16S ribosomal RNA amplicon sequencing.					
34423918	7	60	theme	Blood	767:771	arg1	samples					773:779	Blood samples	767:779	Blood samples	767:779	Blood samples were used for multiplex cytokine analysis and measures of lipopolysaccharide (LPS) and LPS binding protein.					
34423918	14	61	theme	increased	1757:1765	arg1	levels					1777:1782	increased serum LPS levels	1757:1782	increased serum LPS levels	1757:1782	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	4	62	theme	K/L	550:552	arg1	grade					554:558	a K/L grade	548:558	a K/L grade of 0-1 for the knees	548:579	Controls (n = 42) had no hand OA and a K/L grade of 0-1 for the knees.					
34423918	2	63	theme	body	324:327	arg1	BMI					341:343	BMI	341:343	BMI	341:343	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	2	63	theme	body	324:327	arg1	index					334:338	a body mass index	322:338	a body mass index (BMI) ≥30 kg/m2	322:354	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	4	64	contain	had	529:531	arg2	OA					541:542	no hand OA	533:542	no hand OA	533:542	Controls (n = 42) had no hand OA and a K/L grade of 0-1 for the knees.					
34423918	4	64	contain	had	529:531	arg1	Controls					511:518	Controls	511:518	Controls (n = 42)	511:527	Controls (n = 42) had no hand OA and a K/L grade of 0-1 for the knees.					
34423918	4	64	contain	had	529:531	arg1	n = 42					521:526	n = 42	521:526	n = 42	521:526	Controls (n = 42) had no hand OA and a K/L grade of 0-1 for the knees.					
34423918	4	64	contain	had	529:531	arg2	grade					554:558	a K/L grade	548:558	a K/L grade of 0-1 for the knees	548:579	Controls (n = 42) had no hand OA and a K/L grade of 0-1 for the knees.					
34423918	5	65	theme	Compositional	582:594	arg1	analysis					596:603	Compositional analysis	582:603	Compositional analysis of stool samples	582:620	Compositional analysis of stool samples was carried out by 16S ribosomal RNA amplicon sequencing.					
34423918	13	66	from	differences	1653:1663	arg1	severity					1671:1678	OA severity	1668:1678	OA severity	1668:1678	Mice transplanted with patient or control microbiota exhibited a significant difference in α-diversity (P = 0.02) and β-diversity, but no differences in OA severity were observed.					
34423918	14	67	theme	gut	1737:1739	arg1	microbiota					1741:1750	the gut microbiota	1733:1750	the gut microbiota	1733:1750	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	12	68	theme	serum	1470:1474	arg1	P < 0.0001					1481:1490	P < 0.0001	1481:1490	P < 0.0001	1481:1490	Patients had higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001) compared to controls.					
34423918	12	68	theme	serum	1470:1474	arg1	LPS					1476:1478	serum LPS	1470:1478	serum LPS (P < 0.0001)	1470:1491	Patients had higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001) compared to controls.					
34423918	14	69	from	microbiota	1741:1750	arg1	lack					1710:1713	The lack	1706:1713	The lack of differences in the gut microbiota	1706:1750	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	11	70	theme	genus	1351:1355	arg1	composition					1363:1373	genus level composition	1351:1373	genus level composition	1351:1373	There were no significant differences in α- or β-diversity or genus level composition between patients and controls.					
34423918	5	71	theme	amplicon	659:666	arg1	sequencing					668:677	ribosomal RNA amplicon sequencing	645:677	ribosomal RNA amplicon sequencing	645:677	Compositional analysis of stool samples was carried out by 16S ribosomal RNA amplicon sequencing.					
34423918	10	72	dep	higher	1158:1163	arg1	BMI					1170:1172	mean BMI	1165:1172	mean BMI	1165:1172	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	10	72	dep	higher	1158:1163	arg1	score					1255:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	10	72	dep	higher	1158:1163	arg1	grade					1282:1286	higher mean K/L grade	1266:1286	higher mean K/L grade	1266:1286	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	12	73	theme	plasma	1426:1431	arg1	levels					1433:1438	higher plasma levels	1419:1438	higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001)	1419:1491	Patients had higher plasma levels of osteopontin (P = 0.01) and serum LPS (P < 0.0001) compared to controls.					
34423918	1	74	theme	altered	158:164	arg1	dysbiosis					182:190	dysbiosis	182:190	dysbiosis	182:190	OBJECTIVE To test the hypothesis that an altered gut microbiota (dysbiosis) plays a role in obesity-associated osteoarthritis (OA).					
34423918	1	74	theme	altered	158:164	arg1	microbiota					170:179	an altered gut microbiota	155:179	an altered gut microbiota (dysbiosis)	155:191	OBJECTIVE To test the hypothesis that an altered gut microbiota (dysbiosis) plays a role in obesity-associated osteoarthritis (OA).					
34423918	13	75	theme	significant	1580:1590	arg1	difference					1592:1601	a significant difference	1578:1601	a significant difference in α-diversity (P = 0.02) and β-diversity	1578:1643	Mice transplanted with patient or control microbiota exhibited a significant difference in α-diversity (P = 0.02) and β-diversity, but no differences in OA severity were observed.					
34423918	2	76	with	participants	304:315	arg1	BMI					341:343	BMI	341:343	BMI	341:343	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	2	76	with	participants	304:315	arg1	index					334:338	a body mass index	322:338	a body mass index (BMI) ≥30 kg/m2	322:354	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	2	77	dep	index	334:338	arg1	kg/m2					350:354	≥30 kg/m2	346:354	a body mass index (BMI) ≥30 kg/m2	322:354	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	5	78	theme	ribosomal	645:653	arg1	sequencing					668:677	ribosomal RNA amplicon sequencing	645:677	ribosomal RNA amplicon sequencing	645:677	Compositional analysis of stool samples was carried out by 16S ribosomal RNA amplicon sequencing.					
34423918	8	79	theme	%	975:975	arg1	fat					977:979	a 40% fat	971:979	a 40% fat	971:979	Germ-free mice were gavaged with patient- or control-pooled fecal samples and fed a 40% fat, high-sucrose diet for 40 weeks.					
34423918	8	79	theme	%	975:975	arg1	diet					995:998	high-sucrose diet	982:998	high-sucrose diet for 40 weeks	982:1011	Germ-free mice were gavaged with patient- or control-pooled fecal samples and fed a 40% fat, high-sucrose diet for 40 weeks.					
34423918	7	80	theme	protein	880:886	arg1	analysis					814:821	multiplex cytokine analysis	795:821	multiplex cytokine analysis	795:821	Blood samples were used for multiplex cytokine analysis and measures of lipopolysaccharide (LPS) and LPS binding protein.					
34423918	7	80	theme	protein	880:886	arg1	measures					827:834	measures	827:834	measures of lipopolysaccharide (LPS) and LPS binding protein	827:886	Blood samples were used for multiplex cytokine analysis and measures of lipopolysaccharide (LPS) and LPS binding protein.					
34423918	13	81	theme	control	1549:1555	arg1	microbiota					1557:1566	patient or control microbiota	1538:1566	microbiota	1557:1566	Mice transplanted with patient or control microbiota exhibited a significant difference in α-diversity (P = 0.02) and β-diversity, but no differences in OA severity were observed.					
34423918	10	82	theme	mean	1165:1168	arg1	BMI					1170:1172	mean BMI	1165:1172	mean BMI	1165:1172	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	3	83	theme	OA	417:418	arg1	n = 50					430:435	n = 50	430:435	n = 50	430:435	OA patients (n = 50) had hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty).					
34423918	3	83	theme	OA	417:418	arg1	patients					420:427	OA patients	417:427	OA patients (n = 50)	417:436	OA patients (n = 50) had hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty).					
34423918	7	84	theme	LPS	868:870	arg1	protein					880:886	LPS binding protein	868:886	LPS binding protein	868:886	Blood samples were used for multiplex cytokine analysis and measures of lipopolysaccharide (LPS) and LPS binding protein.					
34423918	3	85	dep	OA	456:457	arg1	arthroplasty					496:507	arthroplasty	496:507	arthroplasty	496:507	OA patients (n = 50) had hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty).					
34423918	3	85	dep	OA	456:457	arg1	[K/L					478:481	Kellgren/Lawrence [K/L] grade ≥2	460:491	Kellgren/Lawrence [K/L] grade ≥2	460:491	OA patients (n = 50) had hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty).					
34423918	10	86	theme	Ontario	1195:1201	arg1	score					1255:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	0	87	theme	Serum	25:29	arg1	Lipopolysaccharide					31:48	Increased Serum Lipopolysaccharide	15:48	Increased Serum Lipopolysaccharide	15:48	Association of Increased Serum Lipopolysaccharide, But Not Microbial Dysbiosis, With Obesity-Related Osteoarthritis.					
34423918	14	88	theme	greater	1861:1867	arg1	absorption					1869:1878	greater absorption	1861:1878	greater absorption of LPS, rather than a dysbiotic microbiota,	1861:1922	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	6	89	from	beta-diversity	691:704	arg1	abundances					739:748	taxa relative abundances	725:748	taxa relative abundances	725:748	Alpha- and beta-diversity and differences in taxa relative abundances were determined.					
34423918	2	90	theme	Osteoarthritis	393:406	arg1	Project					408:414	the Johnston County Osteoarthritis Project	373:414	the Johnston County Osteoarthritis Project	373:414	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	10	91	theme	mean	1182:1185	arg1	Ontario					1195:1201	mean Western Ontario	1182:1201	mean Western Ontario	1182:1201	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	2	92	theme	Johnston	377:384	arg1	Project					408:414	the Johnston County Osteoarthritis Project	373:414	the Johnston County Osteoarthritis Project	373:414	METHODS Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m2 , recruited from the Johnston County Osteoarthritis Project.					
34423918	6	93	from	differences	710:720	arg1	abundances					739:748	taxa relative abundances	725:748	taxa relative abundances	725:748	Alpha- and beta-diversity and differences in taxa relative abundances were determined.					
34423918	13	94	theme	patient	1538:1544	arg1	microbiota					1557:1566	patient or control microbiota	1538:1566	microbiota	1557:1566	Mice transplanted with patient or control microbiota exhibited a significant difference in α-diversity (P = 0.02) and β-diversity, but no differences in OA severity were observed.					
34423918	10	95	theme	Osteoarthritis	1229:1242	arg1	score					1255:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
34423918	14	96	theme	intestinal	1824:1833	arg1	permeability					1835:1846	increased intestinal permeability	1814:1846	increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota,	1814:1922	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	14	97	theme	microbiota	1912:1921	arg1	absorption					1869:1878	greater absorption	1861:1878	greater absorption of LPS, rather than a dysbiotic microbiota,	1861:1922	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	14	98	theme	OA	1961:1962	arg1	development					1946:1956	the development	1942:1956	the development of OA associated with obesity	1942:1986	CONCLUSION The lack of differences in the gut microbiota, but increased serum LPS levels, suggest the possibility that increased intestinal permeability allowing for greater absorption of LPS, rather than a dysbiotic microbiota, may contribute to the development of OA associated with obesity.					
34423918	3	99	theme	knee	451:454	arg1	OA					456:457	hand and knee OA	442:457	hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty)	442:508	OA patients (n = 50) had hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty).					
34423918	7	100	theme	lipopolysaccharide	839:856	arg1	analysis					814:821	multiplex cytokine analysis	795:821	multiplex cytokine analysis	795:821	Blood samples were used for multiplex cytokine analysis and measures of lipopolysaccharide (LPS) and LPS binding protein.					
34423918	7	100	theme	lipopolysaccharide	839:856	arg1	measures					827:834	measures	827:834	measures of lipopolysaccharide (LPS) and LPS binding protein	827:886	Blood samples were used for multiplex cytokine analysis and measures of lipopolysaccharide (LPS) and LPS binding protein.					
34423918	6	101	from	Alpha-	680:685	arg1	abundances					739:748	taxa relative abundances	725:748	taxa relative abundances	725:748	Alpha- and beta-diversity and differences in taxa relative abundances were determined.					
34423918	3	102	theme	hand	442:445	arg1	OA					456:457	hand and knee OA	442:457	hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty)	442:508	OA patients (n = 50) had hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty).					
34423918	11	103	from	differences	1315:1325	arg1	composition					1363:1373	genus level composition	1351:1373	genus level composition	1351:1373	There were no significant differences in α- or β-diversity or genus level composition between patients and controls.					
34423918	11	103	from	differences	1315:1325	arg1	β-diversity					1336:1346	β-diversity	1336:1346	β-diversity	1336:1346	There were no significant differences in α- or β-diversity or genus level composition between patients and controls.					
34423918	11	103	from	differences	1315:1325	arg1	α-					1330:1331	α-	1330:1331	α-	1330:1331	There were no significant differences in α- or β-diversity or genus level composition between patients and controls.					
34423918	10	104	theme	pain	1250:1253	arg1	score					1255:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score	1175:1259	RESULTS On average, OA patients were slightly older than the controls, consisted of more women, and had a higher mean BMI, higher mean Western Ontario and McMaster Universities Osteoarthritis Index pain score, and higher mean K/L grade.					
35167953	11	0	dep	evidence	2607:2614	arg1	iv					2603:2604	iv	2603:2604	iv	2603:2604	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	11	1	theme	alginate-gelatin	2354:2369	arg1	microcapsules					2371:2383	alginate-gelatin microcapsules	2354:2383	alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors	2354:2751	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	8	2	theme	malignant	1641:1649	arg1	hints					1651:1655	the most relevant malignant hints	1623:1655	the most relevant malignant hints described in vivo	1623:1673	Overall, our outcomes demonstrate that alginate-gelatin microcapsules represent a simple, yet very accurate tumor-like model, enabling us to mimic the most relevant malignant hints described in vivo, suggesting that confinement and mechanical stress need to be considered when studying pathogenicity and drug resistance of cancer cells in vitro.					
35167953	11	3	from	determination	2393:2405	arg1	determination					2444:2456	(iii) determination	2438:2456	(iii) determination	2438:2456	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	8	4	theme	mechanical	1708:1717	arg1	stress					1719:1724	mechanical stress	1708:1724	mechanical stress	1708:1724	Overall, our outcomes demonstrate that alginate-gelatin microcapsules represent a simple, yet very accurate tumor-like model, enabling us to mimic the most relevant malignant hints described in vivo, suggesting that confinement and mechanical stress need to be considered when studying pathogenicity and drug resistance of cancer cells in vitro.					
35167953	11	5	theme	novel	2151:2155	arg1	outcomes					2157:2164	four novel outcomes	2146:2164	four novel outcomes	2146:2164	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	7	6	theme	increased	1402:1410	arg1	number					1412:1417	the increased number	1398:1417	the increased number of polynucleated cells found within these microcapsules	1398:1473	We finally discuss the role played by aneuploidy in malignancy and resistance to anticancer drugs, based on the increased number of polynucleated cells found within these microcapsules.					
35167953	9	7	theme	microcapsules	1911:1923	arg1	reliability					1879:1889	the reliability	1875:1889	the reliability of alginate-gelatin microcapsules as an artificial tumor model	1875:1952	STATEMENT OF SIGNIFICANCE: In this work, we analyzed the reliability of alginate-gelatin microcapsules as an artificial tumor model.					
35167953	11	8	theme	polymer-based	2721:2733	arg1	tumors					2746:2751	polymer-based artificial tumors	2721:2751	polymer-based artificial tumors	2721:2751	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	6	9	theme	mechanical	1251:1260	arg1	stress					1262:1267	mechanical stress	1251:1267	mechanical stress	1251:1267	Further, we demonstrate that cells isolated from 3D scaffolds show resistance to cisplatin, a process which seems to be strongly influenced by mechanical stress, instead of hypoxia.					
35167953	3	10	from	differences	481:491	arg1	mechano-sensitivity					496:514	mechano-sensitivity	496:514	mechano-sensitivity	496:514	Hydrogel-based microcapsules were initially utilized to detect differences in mechano-sensitivity between MCF7 and MDA-MB-231 breast cancer cells, and the endothelial cell line EA.hy926.					
35167953	4	11	theme	cell	860:863	arg1	morphology					865:874	cell morphology	860:874	cell morphology	860:874	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	1	12	theme	alginate-gelatin	159:174	arg1	microcapsules					176:188	alginate-gelatin microcapsules	159:188	alginate-gelatin microcapsules	159:188	In this work, we analyzed the reliability of alginate-gelatin microcapsules as artificial tumor model.					
35167953	9	13	theme	tumor	1942:1946	arg1	model					1948:1952	an artificial tumor model	1928:1952	an artificial tumor model	1928:1952	STATEMENT OF SIGNIFICANCE: In this work, we analyzed the reliability of alginate-gelatin microcapsules as an artificial tumor model.					
35167953	3	14	theme	endothelial	573:583	arg1	line					590:593	the endothelial cell line EA.hy926	569:602	the endothelial cell line EA.hy926	569:602	Hydrogel-based microcapsules were initially utilized to detect differences in mechano-sensitivity between MCF7 and MDA-MB-231 breast cancer cells, and the endothelial cell line EA.hy926.					
35167953	7	15	theme	anticancer	1371:1380	arg1	drugs					1382:1386	anticancer drugs	1371:1386	anticancer drugs	1371:1386	We finally discuss the role played by aneuploidy in malignancy and resistance to anticancer drugs, based on the increased number of polynucleated cells found within these microcapsules.					
35167953	2	16	theme	cell	358:361	arg1	migration					363:371	-but not hinder- cell migration	341:371	-but not hinder- cell migration	341:371	These tumor-like scaffolds are characterized by their composition and stiffness (∼25 kPa), and their capability to restrict -but not hinder- cell migration, proliferation and release from confinement.					
35167953	4	17	used	used	622:625	arg2	we					619:620	we	619:620	we	619:620	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	1	18	theme	tumor	204:208	arg1	model					210:214	artificial tumor model	193:214	artificial tumor model	193:214	In this work, we analyzed the reliability of alginate-gelatin microcapsules as artificial tumor model.					
35167953	8	19	theme	drug	1780:1783	arg1	resistance					1785:1794	drug resistance	1780:1794	drug resistance	1780:1794	Overall, our outcomes demonstrate that alginate-gelatin microcapsules represent a simple, yet very accurate tumor-like model, enabling us to mimic the most relevant malignant hints described in vivo, suggesting that confinement and mechanical stress need to be considered when studying pathogenicity and drug resistance of cancer cells in vitro.					
35167953	4	20	theme	i.e.	701:704	arg1	3D					713:714	i.e. 2D v/s 3D	701:714	i.e. 2D v/s 3D	701:714	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	4	20	theme	i.e.	701:704	arg1	strategy					691:698	the culture strategy	679:698	the culture strategy (i.e. 2D v/s 3D)	679:715	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	6	21	attach	isolated	1143:1150	arg2	cells					1137:1141	cells	1137:1141	cells isolated from 3D scaffolds	1137:1168	Further, we demonstrate that cells isolated from 3D scaffolds show resistance to cisplatin, a process which seems to be strongly influenced by mechanical stress, instead of hypoxia.					
35167953	6	21	attach	isolated	1143:1150	arg1	scaffolds					1160:1168	3D scaffolds	1157:1168	3D scaffolds	1157:1168	Further, we demonstrate that cells isolated from 3D scaffolds show resistance to cisplatin, a process which seems to be strongly influenced by mechanical stress, instead of hypoxia.					
35167953	8	22	theme	cells	1806:1810	arg1	pathogenicity					1762:1774	pathogenicity	1762:1774	pathogenicity	1762:1774	Overall, our outcomes demonstrate that alginate-gelatin microcapsules represent a simple, yet very accurate tumor-like model, enabling us to mimic the most relevant malignant hints described in vivo, suggesting that confinement and mechanical stress need to be considered when studying pathogenicity and drug resistance of cancer cells in vitro.					
35167953	8	22	theme	cells	1806:1810	arg1	resistance					1785:1794	drug resistance	1780:1794	drug resistance	1780:1794	Overall, our outcomes demonstrate that alginate-gelatin microcapsules represent a simple, yet very accurate tumor-like model, enabling us to mimic the most relevant malignant hints described in vivo, suggesting that confinement and mechanical stress need to be considered when studying pathogenicity and drug resistance of cancer cells in vitro.					
35167953	4	23	theme	v/s	709:711	arg1	3D					713:714	i.e. 2D v/s 3D	701:714	i.e. 2D v/s 3D	701:714	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	4	23	theme	v/s	709:711	arg1	strategy					691:698	the culture strategy	679:698	the culture strategy (i.e. 2D v/s 3D)	679:715	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	5	24	theme	breast	904:909	arg1	cells					918:922	both breast cancer cells	899:922	both breast cancer cells	899:922	Our results show that both breast cancer cells can generate elongated multicellular spheroids inside the microcapsules, prior being released (mimicking intravasation stages), a behavior which was not observed in endothelial cells.					
35167953	11	25	theme	malignant	2250:2258	arg1	cells					2279:2283	malignant and non-pathogenic cells	2250:2283	malignant and non-pathogenic cells	2250:2283	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	2	26	dep	-but	341:344	arg1	hinder-					350:356	hinder-	350:356	hinder-	350:356	These tumor-like scaffolds are characterized by their composition and stiffness (∼25 kPa), and their capability to restrict -but not hinder- cell migration, proliferation and release from confinement.					
35167953	0	27	theme	cell	78:81	arg1	migration					83:91	cell migration	78:91	cell migration	78:91	3D hydrogel-based microcapsules as an in vitro model to study tumorigenicity, cell migration and drug resistance.					
35167953	8	28	theme	alginate-gelatin	1515:1530	arg1	microcapsules					1532:1544	alginate-gelatin microcapsules	1515:1544	alginate-gelatin microcapsules	1515:1544	Overall, our outcomes demonstrate that alginate-gelatin microcapsules represent a simple, yet very accurate tumor-like model, enabling us to mimic the most relevant malignant hints described in vivo, suggesting that confinement and mechanical stress need to be considered when studying pathogenicity and drug resistance of cancer cells in vitro.					
35167953	11	29	dep	studying	2171:2178	arg1	i					2168:2168	i	2168:2168	i	2168:2168	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	3	30	used	utilized	462:469	arg2	microcapsules					433:445	Hydrogel-based microcapsules	418:445	Hydrogel-based microcapsules	418:445	Hydrogel-based microcapsules were initially utilized to detect differences in mechano-sensitivity between MCF7 and MDA-MB-231 breast cancer cells, and the endothelial cell line EA.hy926.					
35167953	0	31	theme	3D	0:1	arg1	hydrogel-based					3:16	3D hydrogel-based	0:16	3D hydrogel-based	0:16	3D hydrogel-based microcapsules as an in vitro model to study tumorigenicity, cell migration and drug resistance.					
35167953	5	32	theme	multicellular	947:959	arg1	spheroids					961:969	elongated multicellular spheroids	937:969	elongated multicellular spheroids	937:969	Our results show that both breast cancer cells can generate elongated multicellular spheroids inside the microcapsules, prior being released (mimicking intravasation stages), a behavior which was not observed in endothelial cells.					
35167953	8	33	theme	simple	1558:1563	arg1	model					1595:1599	a simple, yet very accurate tumor-like model	1556:1599	a simple, yet very accurate tumor-like model	1556:1599	Overall, our outcomes demonstrate that alginate-gelatin microcapsules represent a simple, yet very accurate tumor-like model, enabling us to mimic the most relevant malignant hints described in vivo, suggesting that confinement and mechanical stress need to be considered when studying pathogenicity and drug resistance of cancer cells in vitro.					
35167953	11	34	theme	degree	2421:2426	arg1	determination					2393:2405	determination	2393:2405	determination of malignancy degree in vitro, (iii) determination	2393:2456	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	4	35	theme	genes	747:751	arg1	expression					733:742	the expression	729:742	the expression of genes involved in multidrug resistance	729:784	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	0	36	dep	model	47:51	arg1	study					56:60	study	56:60	to study tumorigenicity, cell migration and drug resistance	53:111	3D hydrogel-based microcapsules as an in vitro model to study tumorigenicity, cell migration and drug resistance.					
35167953	11	37	theme	polynucleated	2684:2696	arg1	cells					2698:2702	polynucleated cells	2684:2702	polynucleated cells localized inside polymer-based artificial tumors	2684:2751	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	8	38	theme	accurate	1575:1582	arg1	model					1595:1599	a simple, yet very accurate tumor-like model	1556:1599	a simple, yet very accurate tumor-like model	1556:1599	Overall, our outcomes demonstrate that alginate-gelatin microcapsules represent a simple, yet very accurate tumor-like model, enabling us to mimic the most relevant malignant hints described in vivo, suggesting that confinement and mechanical stress need to be considered when studying pathogenicity and drug resistance of cancer cells in vitro.					
35167953	11	39	dep	cells	2279:2283	arg1	studying					2291:2298	studying	2291:2298	studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors	2291:2751	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	11	40	theme	anticancer	2635:2644	arg1	drugs					2646:2650	anticancer drugs	2635:2650	anticancer drugs	2635:2650	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	11	41	theme	cell	2304:2307	arg1	morphology					2309:2318	the cell morphology	2300:2318	the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors	2300:2751	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	3	42	theme	MDA-MB-231	533:542	arg1	cells					558:562	MDA-MB-231 breast cancer cells	533:562	MDA-MB-231 breast cancer cells	533:562	Hydrogel-based microcapsules were initially utilized to detect differences in mechano-sensitivity between MCF7 and MDA-MB-231 breast cancer cells, and the endothelial cell line EA.hy926.					
35167953	2	43	from	confinement	405:415	arg1	release					392:398	release	392:398	release from confinement	392:415	These tumor-like scaffolds are characterized by their composition and stiffness (∼25 kPa), and their capability to restrict -but not hinder- cell migration, proliferation and release from confinement.					
35167953	2	43	from	confinement	405:415	arg1	proliferation					374:386	proliferation	374:386	proliferation	374:386	These tumor-like scaffolds are characterized by their composition and stiffness (∼25 kPa), and their capability to restrict -but not hinder- cell migration, proliferation and release from confinement.					
35167953	2	43	from	confinement	405:415	arg1	migration					363:371	-but not hinder- cell migration	341:371	-but not hinder- cell migration	341:371	These tumor-like scaffolds are characterized by their composition and stiffness (∼25 kPa), and their capability to restrict -but not hinder- cell migration, proliferation and release from confinement.					
35167953	11	44	theme	i.e.	2581:2584	arg1	cisplatin					2586:2594	i.e. cisplatin	2581:2594	i.e. cisplatin	2581:2594	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	11	44	theme	i.e.	2581:2584	arg1	drugs					2574:2578	anticancer drugs	2563:2578	anticancer drugs (i.e. cisplatin)	2563:2595	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	2	45	theme	-but	341:344	arg1	migration					363:371	-but not hinder- cell migration	341:371	-but not hinder- cell migration	341:371	These tumor-like scaffolds are characterized by their composition and stiffness (∼25 kPa), and their capability to restrict -but not hinder- cell migration, proliferation and release from confinement.					
35167953	4	46	theme	morphology	865:874	arg1	assays					850:855	assays	850:855	assays of cell morphology	850:874	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	4	46	theme	morphology	865:874	arg1	tests					840:844	cytotoxicological tests	822:844	cytotoxicological tests	822:844	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	11	47	theme	aggregates	2330:2339	arg1	morphology					2309:2318	the cell morphology	2300:2318	the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors	2300:2751	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	7	48	theme	polynucleated	1422:1434	arg1	cells					1436:1440	polynucleated cells	1422:1440	polynucleated cells found within these microcapsules	1422:1473	We finally discuss the role played by aneuploidy in malignancy and resistance to anticancer drugs, based on the increased number of polynucleated cells found within these microcapsules.					
35167953	9	49	theme	alginate-gelatin	1894:1909	arg1	microcapsules					1911:1923	alginate-gelatin microcapsules	1894:1923	alginate-gelatin microcapsules as an artificial tumor model	1894:1952	STATEMENT OF SIGNIFICANCE: In this work, we analyzed the reliability of alginate-gelatin microcapsules as an artificial tumor model.					
35167953	8	50	theme	relevant	1632:1639	arg1	hints					1651:1655	the most relevant malignant hints	1623:1655	the most relevant malignant hints described in vivo	1623:1673	Overall, our outcomes demonstrate that alginate-gelatin microcapsules represent a simple, yet very accurate tumor-like model, enabling us to mimic the most relevant malignant hints described in vivo, suggesting that confinement and mechanical stress need to be considered when studying pathogenicity and drug resistance of cancer cells in vitro.					
35167953	3	51	theme	cancer	551:556	arg1	cells					558:562	MDA-MB-231 breast cancer cells	533:562	MDA-MB-231 breast cancer cells	533:562	Hydrogel-based microcapsules were initially utilized to detect differences in mechano-sensitivity between MCF7 and MDA-MB-231 breast cancer cells, and the endothelial cell line EA.hy926.					
35167953	5	52	located	observed	1077:1084	arg1	cells					1101:1105	endothelial cells	1089:1105	endothelial cells	1089:1105	Our results show that both breast cancer cells can generate elongated multicellular spheroids inside the microcapsules, prior being released (mimicking intravasation stages), a behavior which was not observed in endothelial cells.					
35167953	5	52	located	observed	1077:1084	arg2	behavior					1054:1061	a behavior	1052:1061	a behavior which was not observed in endothelial cells	1052:1105	Our results show that both breast cancer cells can generate elongated multicellular spheroids inside the microcapsules, prior being released (mimicking intravasation stages), a behavior which was not observed in endothelial cells.					
35167953	7	53	theme	cells	1436:1440	arg1	number					1412:1417	the increased number	1398:1417	the increased number of polynucleated cells found within these microcapsules	1398:1473	We finally discuss the role played by aneuploidy in malignancy and resistance to anticancer drugs, based on the increased number of polynucleated cells found within these microcapsules.					
35167953	11	54	theme	non-pathogenic	2264:2277	arg1	cells					2279:2283	malignant and non-pathogenic cells	2250:2283	malignant and non-pathogenic cells	2250:2283	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	11	55	theme	artificial	2735:2744	arg1	tumors					2746:2751	polymer-based artificial tumors	2721:2751	polymer-based artificial tumors	2721:2751	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	3	56	theme	cell	585:588	arg1	line					590:593	the endothelial cell line EA.hy926	569:602	the endothelial cell line EA.hy926	569:602	Hydrogel-based microcapsules were initially utilized to detect differences in mechano-sensitivity between MCF7 and MDA-MB-231 breast cancer cells, and the endothelial cell line EA.hy926.					
35167953	6	57	theme	3D	1157:1158	arg1	scaffolds					1160:1168	3D scaffolds	1157:1168	3D scaffolds	1157:1168	Further, we demonstrate that cells isolated from 3D scaffolds show resistance to cisplatin, a process which seems to be strongly influenced by mechanical stress, instead of hypoxia.					
35167953	9	58	theme	artificial	1931:1940	arg1	model					1948:1952	an artificial tumor model	1928:1952	an artificial tumor model	1928:1952	STATEMENT OF SIGNIFICANCE: In this work, we analyzed the reliability of alginate-gelatin microcapsules as an artificial tumor model.					
35167953	4	59	theme	culture	683:689	arg1	3D					713:714	i.e. 2D v/s 3D	701:714	i.e. 2D v/s 3D	701:714	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	4	59	theme	culture	683:689	arg1	strategy					691:698	the culture strategy	679:698	the culture strategy (i.e. 2D v/s 3D)	679:715	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	1	60	theme	microcapsules	176:188	arg1	reliability					144:154	the reliability	140:154	the reliability of alginate-gelatin microcapsules	140:188	In this work, we analyzed the reliability of alginate-gelatin microcapsules as artificial tumor model.					
35167953	4	61	theme	transcriptomic	639:652	arg1	methods					654:660	RNA-seq and transcriptomic methods	627:660	RNA-seq and transcriptomic methods	627:660	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	2	62	theme	tumor-like	223:232	arg1	scaffolds					234:242	These tumor-like scaffolds	217:242	These tumor-like scaffolds	217:242	These tumor-like scaffolds are characterized by their composition and stiffness (∼25 kPa), and their capability to restrict -but not hinder- cell migration, proliferation and release from confinement.					
35167953	1	63	theme	artificial	193:202	arg1	model					210:214	artificial tumor model	193:214	artificial tumor model	193:214	In this work, we analyzed the reliability of alginate-gelatin microcapsules as artificial tumor model.					
35167953	5	64	dep	released	1009:1016	arg1	mimicking					1019:1027	mimicking	1019:1027	mimicking intravasation stages	1019:1048	Our results show that both breast cancer cells can generate elongated multicellular spheroids inside the microcapsules, prior being released (mimicking intravasation stages), a behavior which was not observed in endothelial cells.					
35167953	5	65	theme	endothelial	1089:1099	arg1	cells					1101:1105	endothelial cells	1089:1105	endothelial cells	1089:1105	Our results show that both breast cancer cells can generate elongated multicellular spheroids inside the microcapsules, prior being released (mimicking intravasation stages), a behavior which was not observed in endothelial cells.					
35167953	5	66	theme	cancer	911:916	arg1	cells					918:922	both breast cancer cells	899:922	both breast cancer cells	899:922	Our results show that both breast cancer cells can generate elongated multicellular spheroids inside the microcapsules, prior being released (mimicking intravasation stages), a behavior which was not observed in endothelial cells.					
35167953	11	67	theme	anticancer	2563:2572	arg1	cisplatin					2586:2594	i.e. cisplatin	2581:2594	i.e. cisplatin	2581:2594	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	11	67	theme	anticancer	2563:2572	arg1	drugs					2574:2578	anticancer drugs	2563:2578	anticancer drugs (i.e. cisplatin)	2563:2595	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	7	68	located	found	1442:1446	arg2	cells					1436:1440	polynucleated cells	1422:1440	polynucleated cells found within these microcapsules	1422:1473	We finally discuss the role played by aneuploidy in malignancy and resistance to anticancer drugs, based on the increased number of polynucleated cells found within these microcapsules.					
35167953	7	68	located	found	1442:1446	arg1	microcapsules					1461:1473	these microcapsules	1455:1473	these microcapsules	1455:1473	We finally discuss the role played by aneuploidy in malignancy and resistance to anticancer drugs, based on the increased number of polynucleated cells found within these microcapsules.					
35167953	11	69	theme	resistant	2550:2558	arg1	clones					2543:2548	clones	2543:2548	clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors	2543:2751	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	11	70	theme	cells	2698:2702	arg1	presence					2672:2679	the presence	2668:2679	the presence of polynucleated cells localized inside polymer-based artificial tumors	2668:2751	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	10	71	theme	elastic	2011:2017	arg1	properties					2019:2028	elastic properties	2011:2028	elastic properties	2011:2028	These scaffolds are characterized by their composition, elastic properties, and their ability to restrict cell migration, proliferation, and release from confinement.					
35167953	8	72	theme	cancer	1799:1804	arg1	cells					1806:1810	cancer cells	1799:1810	cancer cells	1799:1810	Overall, our outcomes demonstrate that alginate-gelatin microcapsules represent a simple, yet very accurate tumor-like model, enabling us to mimic the most relevant malignant hints described in vivo, suggesting that confinement and mechanical stress need to be considered when studying pathogenicity and drug resistance of cancer cells in vitro.					
35167953	11	73	theme	mechanical	2479:2488	arg1	stress					2490:2495	mechanical stress	2479:2495	mechanical stress	2479:2495	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	4	74	theme	2D	706:707	arg1	3D					713:714	i.e. 2D v/s 3D	701:714	i.e. 2D v/s 3D	701:714	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	4	74	theme	2D	706:707	arg1	strategy					691:698	the culture strategy	679:698	the culture strategy (i.e. 2D v/s 3D)	679:715	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	0	75	theme	drug	97:100	arg1	resistance					102:111	drug resistance	97:111	drug resistance	97:111	3D hydrogel-based microcapsules as an in vitro model to study tumorigenicity, cell migration and drug resistance.					
35167953	4	76	theme	multidrug	765:773	arg1	resistance					775:784	multidrug resistance	765:784	multidrug resistance	765:784	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	5	77	theme	elongated	937:945	arg1	spheroids					961:969	elongated multicellular spheroids	937:969	elongated multicellular spheroids	937:969	Our results show that both breast cancer cells can generate elongated multicellular spheroids inside the microcapsules, prior being released (mimicking intravasation stages), a behavior which was not observed in endothelial cells.					
35167953	11	78	theme	malignancy	2410:2419	arg1	degree					2421:2426	malignancy degree	2410:2426	malignancy degree	2410:2426	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	11	79	dep	determination	2444:2456	arg1	iii					2439:2441	iii	2439:2441	iii	2439:2441	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	11	79	dep	determination	2444:2456	arg1	vitro					2431:2435	vitro, (iii) determination	2431:2456	vitro	2431:2435	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	3	80	theme	Hydrogel-based	418:431	arg1	microcapsules					433:445	Hydrogel-based microcapsules	418:445	Hydrogel-based microcapsules	418:445	Hydrogel-based microcapsules were initially utilized to detect differences in mechano-sensitivity between MCF7 and MDA-MB-231 breast cancer cells, and the endothelial cell line EA.hy926.					
35167953	0	81	theme	in	38:39	arg1	model					47:51	an in vitro model	35:51	an in vitro model to study tumorigenicity, cell migration and drug resistance	35:111	3D hydrogel-based microcapsules as an in vitro model to study tumorigenicity, cell migration and drug resistance.					
35167953	5	82	theme	intravasation	1029:1041	arg1	stages					1043:1048	intravasation stages	1029:1048	intravasation stages	1029:1048	Our results show that both breast cancer cells can generate elongated multicellular spheroids inside the microcapsules, prior being released (mimicking intravasation stages), a behavior which was not observed in endothelial cells.					
35167953	9	83	theme	SIGNIFICANCE	1835:1846	arg1	STATEMENT					1822:1830	STATEMENT	1822:1830	STATEMENT OF SIGNIFICANCE	1822:1846	STATEMENT OF SIGNIFICANCE: In this work, we analyzed the reliability of alginate-gelatin microcapsules as an artificial tumor model.					
35167953	8	84	theme	tumor-like	1584:1593	arg1	model					1595:1599	a simple, yet very accurate tumor-like model	1556:1599	a simple, yet very accurate tumor-like model	1556:1599	Overall, our outcomes demonstrate that alginate-gelatin microcapsules represent a simple, yet very accurate tumor-like model, enabling us to mimic the most relevant malignant hints described in vivo, suggesting that confinement and mechanical stress need to be considered when studying pathogenicity and drug resistance of cancer cells in vitro.					
35167953	0	85	dep	in	38:39	arg1	vitro					41:45	vitro	41:45	vitro	41:45	3D hydrogel-based microcapsules as an in vitro model to study tumorigenicity, cell migration and drug resistance.					
35167953	11	86	theme	cell	2180:2183	arg1	migration					2185:2193	cell migration	2180:2193	cell migration	2180:2193	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	4	87	theme	cytotoxicological	822:838	arg1	tests					840:844	cytotoxicological tests	822:844	cytotoxicological tests	822:844	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	11	88	theme	cancer	2323:2328	arg1	aggregates					2330:2339	cancer aggregates	2323:2339	cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors	2323:2751	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	10	89	theme	cell	2061:2064	arg1	migration					2066:2074	cell migration	2061:2074	cell migration	2061:2074	These scaffolds are characterized by their composition, elastic properties, and their ability to restrict cell migration, proliferation, and release from confinement.					
35167953	4	90	theme	RNA-seq	627:633	arg1	methods					654:660	RNA-seq and transcriptomic methods	627:660	RNA-seq and transcriptomic methods	627:660	Additionally, we used RNA-seq and transcriptomic methods to determine how the culture strategy (i.e. 2D v/s 3D) may pre-set the expression of genes involved in multidrug resistance, being then validated by performing cytotoxicological tests and assays of cell morphology.					
35167953	11	91	dep	studying	2291:2298	arg1	ii					2287:2288	ii	2287:2288	ii	2287:2288	Our results demonstrate four novel outcomes: (i) studying cell migration and proliferation in 3D enabled discrimination between malignant and non-pathogenic cells, (ii) studying the cell morphology of cancer aggregates entrapped in alginate-gelatin microcapsules enabled determination of malignancy degree in vitro, (iii) determination that confinement and mechanical stress, instead of hypoxia, are required to generate clones resistant to anticancer drugs (i.e. cisplatin), and (iv) evidence that resistance to anticancer drugs could be due to the presence of polynucleated cells localized inside polymer-based artificial tumors.					
35167953	3	92	theme	breast	544:549	arg1	cells					558:562	MDA-MB-231 breast cancer cells	533:562	MDA-MB-231 breast cancer cells	533:562	Hydrogel-based microcapsules were initially utilized to detect differences in mechano-sensitivity between MCF7 and MDA-MB-231 breast cancer cells, and the endothelial cell line EA.hy926.					
36987289	5	0	dep	classes	783:789	arg1	biopolymers					792:802	biopolymers	792:802	biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum)	792:913	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	5	0	dep	classes	783:789	arg1	ceramics					919:926	ceramics	919:926	ceramics (more specific, calcium phosphates)	919:962	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	5	0	dep	classes	783:789	arg1	specific					934:941	specific	934:941	specific	934:941	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	5	1	theme	composites	665:674	arg1	CBs					683:685	CBs	683:685	CBs	683:685	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	5	1	theme	composites	665:674	arg1	beads					676:680	composites beads	665:680	composites beads (CBs) with application in the complex field of BTE	665:731	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	8	2	theme	guar	1562:1565	arg1	gum					1567:1569	guar gum	1562:1569	guar gum	1562:1569	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	6	3	dep	in	1233:1234	arg1	vitro					1236:1240	vitro	1236:1240	vitro	1236:1240	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	7	4	theme	cytotoxicity	1421:1432	arg1	free					1413:1416	free	1413:1416	free	1413:1416	Moreover, preliminary in vitro studies on cell cultures highlighted that the CBs were free of cytotoxicity and did not affect the morphology and density of cells.					
36987289	2	5	theme	chronic	311:317	arg1	disease-mineral					326:340	chronic kidney disease-mineral and bone disorders	311:359	disease-mineral	326:340	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	6	6	from	strength	1219:1226	arg1	fluids					1295:1300	four different acellular simulated body fluids	1255:1300	four different acellular simulated body fluids	1255:1300	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	6	7	theme	physico-chemical	1109:1124	arg1	characteristics					1126:1140	adequate physico-chemical characteristics	1100:1140	adequate physico-chemical characteristics	1100:1140	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	8	8	from	fluids	1712:1717	arg1	terms					1649:1653	terms	1649:1653	terms of mechanical properties and behaviour in simulated body fluids	1649:1717	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	2	9	theme	bone	346:349	arg1	disorders					351:359	chronic kidney disease-mineral and bone disorders	311:359	disorders	351:359	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	8	10	theme	properties	1669:1678	arg1	terms					1649:1653	terms	1649:1653	terms of mechanical properties and behaviour in simulated body fluids	1649:1717	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	7	11	theme	in	1349:1350	arg1	studies					1358:1364	preliminary in vitro studies	1337:1364	preliminary in vitro studies on cell cultures highlighted that the CBs	1337:1406	Moreover, preliminary in vitro studies on cell cultures highlighted that the CBs were free of cytotoxicity and did not affect the morphology and density of cells.					
36987289	8	12	theme	behaviour	1684:1692	arg1	terms					1649:1653	terms	1649:1653	terms of mechanical properties and behaviour in simulated body fluids	1649:1717	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	1	13	theme	aging	137:141	arg1	tendency					114:121	The tendency	110:121	The tendency of population aging	110:141	The tendency of population aging is continuously increasing, which is directly correlated with a significative number of associated pathologies.					
36987289	6	14	theme	simulated	1280:1288	arg1	fluids					1295:1300	four different acellular simulated body fluids	1255:1300	four different acellular simulated body fluids	1255:1300	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	2	15	theme	Several	255:261	arg1	diseases					278:285	Several metabolic bone diseases	255:285	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders	255:359	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	2	15	theme	Several	255:261	arg1	osteoporosis					295:306	osteoporosis	295:306	osteoporosis	295:306	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	2	15	theme	Several	255:261	arg1	disease-mineral					326:340	chronic kidney disease-mineral and bone disorders	311:359	disease-mineral	326:340	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	8	16	theme	simulated	1697:1705	arg1	fluids					1712:1717	simulated body fluids	1697:1717	simulated body fluids	1697:1717	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	1	17	theme	associated	231:240	arg1	pathologies					242:252	associated pathologies	231:252	associated pathologies	231:252	The tendency of population aging is continuously increasing, which is directly correlated with a significative number of associated pathologies.					
36987289	5	18	dep	specific	934:941	arg1	phosphates					952:961	calcium phosphates	944:961	calcium phosphates	944:961	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	6	19	theme	different	1260:1268	arg1	fluids					1295:1300	four different acellular simulated body fluids	1255:1300	four different acellular simulated body fluids	1255:1300	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	5	20	theme	guar	882:885	arg1	gum					910:912	guar gum/carboxymethyl guar gum	882:912	guar gum/carboxymethyl guar gum	882:912	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	5	21	theme	first	1000:1004	arg1	time					1006:1009	the first time	996:1009	the first time in the literature	996:1027	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	5	22	theme	guar	905:908	arg1	gum					910:912	guar gum/carboxymethyl guar gum	882:912	guar gum/carboxymethyl guar gum	882:912	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	7	23	from	studies	1358:1364	arg1	cultures					1374:1381	cell cultures	1369:1381	cell cultures highlighted that the CBs	1369:1406	Moreover, preliminary in vitro studies on cell cultures highlighted that the CBs were free of cytotoxicity and did not affect the morphology and density of cells.					
36987289	5	24	from	time	1006:1009	arg1	literature					1018:1027	the literature	1014:1027	the literature	1014:1027	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	2	25	theme	bone	273:276	arg1	diseases					278:285	Several metabolic bone diseases	255:285	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders	255:359	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	2	25	theme	bone	273:276	arg1	osteoporosis					295:306	osteoporosis	295:306	osteoporosis	295:306	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	2	25	theme	bone	273:276	arg1	disease-mineral					326:340	chronic kidney disease-mineral and bone disorders	311:359	disease-mineral	326:340	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	4	26	theme	bone	537:540	arg1	BTE					562:564	BTE	562:564	BTE	562:564	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	4	26	theme	bone	537:540	arg1	engineering					549:559	bone tissue engineering	537:559	bone tissue engineering (BTE) strategy	537:574	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	8	27	contain	have	1571:1574	arg1	beads					1521:1525	the beads	1517:1525	the beads based on a higher concentration of guar gum	1517:1569	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	8	27	contain	have	1571:1574	arg2	properties					1585:1594	superior properties	1576:1594	superior properties	1576:1594	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	6	28	from	morphology	1160:1169	arg1	fluids					1295:1300	four different acellular simulated body fluids	1255:1300	four different acellular simulated body fluids	1255:1300	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	5	29	theme	complex	712:718	arg1	field					720:724	the complex field	708:724	the complex field of BTE	708:731	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	3	30	theme	supportive	455:464	arg1	treatments					466:475	self-heal and supportive treatments	441:475	self-heal and supportive treatments	441:475	Due to the specific fragility, bones will not self-heal and supportive treatments are necessary.					
36987289	4	31	theme	efficient	593:601	arg1	bone					504:507	Implantable bone	492:507	Implantable bone substitutes	492:519	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	4	31	theme	efficient	593:601	arg1	solution					603:610	an efficient solution	590:610	an efficient solution for this issue	590:625	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	3	32	theme	specific	406:413	arg1	fragility					415:423	the specific fragility	402:423	the specific fragility	402:423	Due to the specific fragility, bones will not self-heal and supportive treatments are necessary.					
36987289	3	33	theme	self-heal	441:449	arg1	treatments					466:475	self-heal and supportive treatments	441:475	self-heal and supportive treatments	441:475	Due to the specific fragility, bones will not self-heal and supportive treatments are necessary.					
36987289	5	34	with	beads	676:680	arg1	application					693:703	application	693:703	application in the complex field of BTE	693:731	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	4	35	theme	strategy	567:574	arg1	bone					504:507	Implantable bone	492:507	Implantable bone substitutes	492:519	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	4	35	theme	strategy	567:574	arg1	component					524:532	a component	522:532	a component of bone tissue engineering (BTE) strategy	522:574	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	0	36	theme	Composite	35:43	arg1	Beads					45:49	Composite Beads	35:49	Composite Beads	35:49	Polysaccharides-Calcium Phosphates Composite Beads as Bone Substitutes for Fractures Repair and Regeneration.					
36987289	7	37	theme	cell	1369:1372	arg1	cultures					1374:1381	cell cultures	1369:1381	cell cultures highlighted that the CBs	1369:1406	Moreover, preliminary in vitro studies on cell cultures highlighted that the CBs were free of cytotoxicity and did not affect the morphology and density of cells.					
36987289	0	38	dep	Fractures	75:83	arg1	Fractures					75:83	Fractures Repair and Regeneration	75:107	Fractures Repair and Regeneration	75:107	Polysaccharides-Calcium Phosphates Composite Beads as Bone Substitutes for Fractures Repair and Regeneration.					
36987289	0	38	dep	Fractures	75:83	arg1	Regeneration					96:107	Regeneration	96:107	Regeneration	96:107	Polysaccharides-Calcium Phosphates Composite Beads as Bone Substitutes for Fractures Repair and Regeneration.					
36987289	0	38	dep	Fractures	75:83	arg1	Repair					85:90	Repair	85:90	Repair	85:90	Polysaccharides-Calcium Phosphates Composite Beads as Bone Substitutes for Fractures Repair and Regeneration.					
36987289	7	39	dep	morphology	1457:1466	arg1	the					1453:1455	the	1453:1455	the	1453:1455	Moreover, preliminary in vitro studies on cell cultures highlighted that the CBs were free of cytotoxicity and did not affect the morphology and density of cells.					
36987289	4	40	theme	engineering	549:559	arg1	strategy					567:574	bone tissue engineering (BTE) strategy	537:574	bone tissue engineering (BTE) strategy	537:574	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	8	41	from	behaviour	1684:1692	arg1	fluids					1712:1717	simulated body fluids	1697:1717	simulated body fluids	1697:1717	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	8	42	theme	superior	1576:1583	arg1	properties					1585:1594	superior properties	1576:1594	superior properties	1576:1594	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	1	43	theme	pathologies	242:252	arg1	number					221:226	a significative number	205:226	a significative number of associated pathologies	205:252	The tendency of population aging is continuously increasing, which is directly correlated with a significative number of associated pathologies.					
36987289	6	44	theme	double	1050:1055	arg1	crosslinking					1057:1068	double crosslinking	1050:1068	double crosslinking	1050:1068	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	8	45	from	properties	1669:1678	arg1	fluids					1712:1717	simulated body fluids	1697:1717	simulated body fluids	1697:1717	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	6	46	from	behaviour	1242:1250	arg1	fluids					1295:1300	four different acellular simulated body fluids	1255:1300	four different acellular simulated body fluids	1255:1300	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	8	47	theme	higher	1538:1543	arg1	concentration					1545:1557	a higher concentration	1536:1557	a higher concentration of guar gum	1536:1569	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	5	48	theme	study	644:648	arg1	aim					632:634	The aim	628:634	The aim of this study	628:648	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	2	49	theme	fractures	384:392	arg1	risk					376:379	a high risk	369:379	a high risk of fractures	369:392	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	5	50	from	application	693:703	arg1	field					720:724	the complex field	708:724	the complex field of BTE	708:731	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	6	51	theme	in	1233:1234	arg1	capabilities					1146:1157	capabilities	1146:1157	capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids)	1146:1301	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	6	51	theme	in	1233:1234	arg1	behaviour					1242:1250	in vitro behaviour	1233:1250	in vitro behaviour	1233:1250	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	2	52	theme	kidney	319:324	arg1	disease-mineral					326:340	chronic kidney disease-mineral and bone disorders	311:359	disease-mineral	326:340	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	8	53	theme	gum	1567:1569	arg1	concentration					1545:1557	a higher concentration	1536:1557	a higher concentration of guar gum	1536:1569	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	6	54	theme	adequate	1100:1107	arg1	characteristics					1126:1140	adequate physico-chemical characteristics	1100:1140	adequate physico-chemical characteristics	1100:1140	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	6	55	theme	mechanical	1208:1217	arg1	strength					1219:1226	mechanical strength	1208:1226	mechanical strength	1208:1226	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	6	55	theme	mechanical	1208:1217	arg1	capabilities					1146:1157	capabilities	1146:1157	capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids)	1146:1301	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	8	56	theme	mechanical	1658:1667	arg1	properties					1669:1678	mechanical properties	1658:1678	mechanical properties	1658:1678	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	1	57	theme	significative	207:219	arg1	number					221:226	a significative number	205:226	a significative number of associated pathologies	205:252	The tendency of population aging is continuously increasing, which is directly correlated with a significative number of associated pathologies.					
36987289	6	58	theme	body	1290:1293	arg1	fluids					1295:1300	four different acellular simulated body fluids	1255:1300	four different acellular simulated body fluids	1255:1300	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	2	59	theme	metabolic	263:271	arg1	diseases					278:285	Several metabolic bone diseases	255:285	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders	255:359	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	2	59	theme	metabolic	263:271	arg1	osteoporosis					295:306	osteoporosis	295:306	osteoporosis	295:306	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	2	59	theme	metabolic	263:271	arg1	disease-mineral					326:340	chronic kidney disease-mineral and bone disorders	311:359	disease-mineral	326:340	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	4	60	theme	Implantable	492:502	arg1	bone					504:507	Implantable bone	492:507	Implantable bone substitutes	492:519	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	4	60	theme	Implantable	492:502	arg1	solution					603:610	an efficient solution	590:610	an efficient solution for this issue	590:625	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	4	60	theme	Implantable	492:502	arg1	component					524:532	a component	522:532	a component of bone tissue engineering (BTE) strategy	522:574	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	7	61	theme	preliminary	1337:1347	arg1	studies					1358:1364	preliminary in vitro studies	1337:1364	preliminary in vitro studies on cell cultures highlighted that the CBs	1337:1406	Moreover, preliminary in vitro studies on cell cultures highlighted that the CBs were free of cytotoxicity and did not affect the morphology and density of cells.					
36987289	6	62	theme	acellular	1270:1278	arg1	fluids					1295:1300	four different acellular simulated body fluids	1255:1300	four different acellular simulated body fluids	1255:1300	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	1	63	theme	population	126:135	arg1	aging					137:141	population aging	126:141	population aging	126:141	The tendency of population aging is continuously increasing, which is directly correlated with a significative number of associated pathologies.					
36987289	6	64	theme	chemical	1172:1179	arg1	structure					1181:1189	chemical structure	1172:1189	chemical structure	1172:1189	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	6	64	theme	chemical	1172:1179	arg1	capabilities					1146:1157	capabilities	1146:1157	capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids)	1146:1301	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	6	65	dep	capabilities	1146:1157	arg1	morphology					1160:1169	morphology	1160:1169	morphology	1160:1169	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	6	65	dep	capabilities	1146:1157	arg1	structure					1181:1189	chemical structure	1172:1189	chemical structure	1172:1189	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	6	65	dep	capabilities	1146:1157	arg1	capabilities					1146:1157	capabilities	1146:1157	capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids)	1146:1301	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	6	65	dep	capabilities	1146:1157	arg1	strength					1219:1226	mechanical strength	1208:1226	mechanical strength	1208:1226	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	6	65	dep	capabilities	1146:1157	arg1	behaviour					1242:1250	in vitro behaviour	1233:1250	in vitro behaviour	1233:1250	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	6	66	from	structure	1181:1189	arg1	fluids					1295:1300	four different acellular simulated body fluids	1255:1300	four different acellular simulated body fluids	1255:1300	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	8	67	theme	body	1707:1710	arg1	fluids					1712:1717	simulated body fluids	1697:1717	simulated body fluids	1697:1717	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	5	68	theme	gum/carboxymethyl	887:903	arg1	gum					910:912	guar gum/carboxymethyl guar gum	882:912	guar gum/carboxymethyl guar gum	882:912	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	6	69	from	composition	1195:1205	arg1	fluids					1295:1300	four different acellular simulated body fluids	1255:1300	four different acellular simulated body fluids	1255:1300	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	5	70	theme	BTE	729:731	arg1	field					720:724	the complex field	708:724	the complex field of BTE	708:731	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	7	71	dep	in	1349:1350	arg1	vitro					1352:1356	vitro	1352:1356	vitro	1352:1356	Moreover, preliminary in vitro studies on cell cultures highlighted that the CBs were free of cytotoxicity and did not affect the morphology and density of cells.					
36987289	6	72	theme	tissue	1312:1317	arg1	repair					1319:1324	bone tissue repair	1307:1324	bone tissue repair	1307:1324	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	5	73	theme	different	854:862	arg1	concentrations					864:877	two different concentrations	850:877	two different concentrations of guar gum/carboxymethyl guar gum	850:912	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	4	74	theme	tissue	542:547	arg1	BTE					562:564	BTE	562:564	BTE	562:564	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	4	74	theme	tissue	542:547	arg1	engineering					549:559	bone tissue engineering	537:559	bone tissue engineering (BTE) strategy	537:574	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	6	75	theme	bone	1307:1310	arg1	repair					1319:1324	bone tissue repair	1307:1324	bone tissue repair	1307:1324	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	3	76	dep	bones	426:430	arg1	will					432:435	will	432:435	will	432:435	Due to the specific fragility, bones will not self-heal and supportive treatments are necessary.					
36987289	5	77	theme	gum	910:912	arg1	concentrations					864:877	two different concentrations	850:877	two different concentrations of guar gum/carboxymethyl guar gum	850:912	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	5	77	theme	gum	910:912	arg1	alginate					837:844	alginate	837:844	alginate	837:844	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	5	78	dep	biopolymers	792:802	arg1	polysaccharides					820:834	polysaccharides	820:834	polysaccharides	820:834	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	5	78	dep	biopolymers	792:802	arg1	specific					810:817	specific	810:817	specific	810:817	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	4	79	dep	bone	504:507	arg1	substitutes					509:519	substitutes	509:519	substitutes	509:519	Implantable bone substitutes, a component of bone tissue engineering (BTE) strategy, proved to be an efficient solution for this issue.					
36987289	7	80	theme	cells	1483:1487	arg1	density					1472:1478	density	1472:1478	density	1472:1478	Moreover, preliminary in vitro studies on cell cultures highlighted that the CBs were free of cytotoxicity and did not affect the morphology and density of cells.					
36987289	7	80	theme	cells	1483:1487	arg1	morphology					1457:1466	morphology	1457:1466	morphology	1457:1466	Moreover, preliminary in vitro studies on cell cultures highlighted that the CBs were free of cytotoxicity and did not affect the morphology and density of cells.					
36987289	8	81	from	terms	1649:1653	arg1	fluids					1712:1717	simulated body fluids	1697:1717	simulated body fluids	1697:1717	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36987289	6	82	theme	ionic	1071:1075	arg1	CBs					1034:1036	The CBs	1030:1036	The CBs prepared by double crosslinking (ionic and physically)	1030:1091	The CBs prepared by double crosslinking (ionic and physically) showed adequate physico-chemical characteristics and capabilities (morphology, chemical structure and composition, mechanical strength, and in vitro behaviour in four different acellular simulated body fluids) for bone tissue repair.					
36987289	2	83	theme	high	371:374	arg1	risk					376:379	a high risk	369:379	a high risk of fractures	369:392	Several metabolic bone diseases such as osteoporosis or chronic kidney disease-mineral and bone disorders involve a high risk of fractures.					
36987289	5	84	theme	calcium	944:950	arg1	phosphates					952:961	calcium phosphates	944:961	calcium phosphates	944:961	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	5	85	dep	specific	810:817	arg1	concentrations					864:877	two different concentrations	850:877	two different concentrations of guar gum/carboxymethyl guar gum	850:912	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	5	85	dep	specific	810:817	arg1	alginate					837:844	alginate	837:844	alginate	837:844	The aim of this study was to develop composites beads (CBs) with application in the complex field of BTE, by assembling the features of both biomaterials' classes: biopolymers (more specific, polysaccharides: alginate and two different concentrations of guar gum/carboxymethyl guar gum) and ceramics (more specific, calcium phosphates), in a combination described for the first time in the literature.					
36987289	8	86	theme	carboxymetilated	1612:1627	arg1	guar					1629:1632	carboxymetilated guar	1612:1632	carboxymetilated guar	1612:1632	The results indicated that the beads based on a higher concentration of guar gum have superior properties than those with carboxymetilated guar, especially in terms of mechanical properties and behaviour in simulated body fluids.					
36207726	0	0	theme	Mentha	80:85	arg1	nanofibers					128:137	Mentha spicata L. essential oil and electrospun nanofibers	80:137	Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose	80:187	Antioxidative, anticancer, and antibacterial activities of a nanogel containing Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose.					
36207726	12	1	theme	lesion	1906:1911	arg1	mat					1829:1831	the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat	1746:1831	the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects	1746:1888	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	12	1	theme	lesion	1906:1911	arg1	dressing					1913:1920	lesion dressing	1906:1920	lesion dressing after treatment with the nanogel	1906:1953	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	10	2	theme	nanogel	1465:1471	arg1	potency					1450:1456	the potency	1446:1456	the potency of the nanogel with an IC50 value of 55.0 µg/mL	1446:1504	Furthermore, the potency of the nanogel with an IC50 value of 55.0 µg/mL was substantially more (P < 0.001) than the essential oil (997.4 µg/mL).					
36207726	10	2	theme	nanogel	1465:1471	arg1	more					1524:1527	more	1524:1527	more	1524:1527	Furthermore, the potency of the nanogel with an IC50 value of 55.0 µg/mL was substantially more (P < 0.001) than the essential oil (997.4 µg/mL).					
36207726	11	3	theme	aureus	1614:1619	arg1	growth					1589:1594	the growth	1585:1594	the growth of Staphylococcus aureus and Escherichia coli after treatment with 1000 µg/mL nanogel	1585:1680	Also, the growth of Staphylococcus aureus and Escherichia coli after treatment with 1000 µg/mL nanogel was about 50% decreased compared to the control group.					
36207726	13	4	theme	natural	1970:1976	arg1	potency					1961:1967	High potency	1956:1967	High potency	1956:1967	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	13	4	theme	natural	1970:1976	arg1	ingredients					1978:1988	natural ingredients	1970:1988	natural ingredients	1970:1988	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	13	4	theme	natural	1970:1976	arg1	advantages					2027:2036	advantages	2027:2036	advantages of the prepared nanogel	2027:2060	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	13	4	theme	natural	1970:1976	arg1	preparation					2011:2021	straightforward preparation	1995:2021	straightforward preparation	1995:2021	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	4	5	theme	successful	683:692	arg1	loading					694:700	successful loading	683:700	successful loading of the essential oil in the nanogel	683:736	Its nanoemulsion-based nanogel was then investigated; successful loading of the essential oil in the nanogel was confirmed using FTIR analysis.					
36207726	9	6	from	concentrations	1399:1412	arg1	potent					1341:1346	potent	1341:1346	potent	1341:1346	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	9	6	from	concentrations	1399:1412	arg1	P < 0.001					1372:1380	P < 0.001	1372:1380	P < 0.001	1372:1380	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	9	6	from	concentrations	1399:1412	arg1	effect					1296:1301	The antioxidant effect	1280:1301	The antioxidant effect of the nanogel	1280:1316	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	9	7	theme	antioxidant	1284:1294	arg1	potent					1341:1346	potent	1341:1346	potent	1341:1346	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	9	7	theme	antioxidant	1284:1294	arg1	P < 0.001					1372:1380	P < 0.001	1372:1380	P < 0.001	1372:1380	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	9	7	theme	antioxidant	1284:1294	arg1	effect					1296:1301	The antioxidant effect	1280:1301	The antioxidant effect of the nanogel	1280:1316	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	0	8	theme	L.	95:96	arg1	nanofibers					128:137	Mentha spicata L. essential oil and electrospun nanofibers	80:137	Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose	80:187	Antioxidative, anticancer, and antibacterial activities of a nanogel containing Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose.					
36207726	2	9	used	used	455:458	arg2	nanogels					398:405	natural nanogels	390:405	natural nanogels that, besides hydrating the skin, could also be used as antioxidant or antibacterial agents	390:497	Recently, many attempts have been made to develop natural nanogels that, besides hydrating the skin, could also be used as antioxidant or antibacterial agents.					
36207726	2	9	used	used	455:458	arg2	antioxidant					463:473	antioxidant	463:473	antioxidant	463:473	Recently, many attempts have been made to develop natural nanogels that, besides hydrating the skin, could also be used as antioxidant or antibacterial agents.					
36207726	2	9	used	used	455:458	arg2	agents					492:497	antibacterial agents	478:497	antibacterial agents	478:497	Recently, many attempts have been made to develop natural nanogels that, besides hydrating the skin, could also be used as antioxidant or antibacterial agents.					
36207726	12	10	theme	polycaprolactone-hydroxypropyl	1771:1800	arg1	mat					1829:1831	the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat	1746:1831	the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects	1746:1888	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	12	10	theme	polycaprolactone-hydroxypropyl	1771:1800	arg1	dressing					1913:1920	lesion dressing	1906:1920	lesion dressing after treatment with the nanogel	1906:1953	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	12	11	theme	cytotoxic	1841:1849	arg1	effects					1882:1888	no cytotoxic, antioxidant, or antibacterial effects	1838:1888	no cytotoxic, antioxidant, or antibacterial effects	1838:1888	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	1	12	theme	chemical	272:279	arg1	materials					281:289	chemical materials	272:289	chemical materials	272:289	BACKGROUND As the largest organ, the skin has been frequently affected by trauma, chemical materials, toxins, bacterial pathogens, and free radicals.					
36207726	0	13	theme	spicata	87:93	arg1	nanofibers					128:137	Mentha spicata L. essential oil and electrospun nanofibers	80:137	Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose	80:187	Antioxidative, anticancer, and antibacterial activities of a nanogel containing Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose.					
36207726	12	14	theme	electrospun	1759:1769	arg1	mat					1829:1831	the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat	1746:1831	the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects	1746:1888	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	12	14	theme	electrospun	1759:1769	arg1	dressing					1913:1920	lesion dressing	1906:1920	lesion dressing after treatment with the nanogel	1906:1953	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	13	15	theme	straightforward	1995:2009	arg1	potency					1961:1967	High potency	1956:1967	High potency	1956:1967	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	13	15	theme	straightforward	1995:2009	arg1	ingredients					1978:1988	natural ingredients	1970:1988	natural ingredients	1970:1988	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	13	15	theme	straightforward	1995:2009	arg1	advantages					2027:2036	advantages	2027:2036	advantages of the prepared nanogel	2027:2060	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	13	15	theme	straightforward	1995:2009	arg1	preparation					2011:2021	straightforward preparation	1995:2021	straightforward preparation	1995:2021	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	2	16	dep	many	350:353	arg1	attempts					355:362	attempts	355:362	attempts	355:362	Recently, many attempts have been made to develop natural nanogels that, besides hydrating the skin, could also be used as antioxidant or antibacterial agents.					
36207726	7	17	theme	196 ± 8 nm	1143:1152	arg1	size					1135:1138	droplet size	1127:1138	droplet size of 196 ± 8 nm	1127:1152	By adding carboxymethylcellulose (3.5% w/v) to the optimal nanoemulsion containing the essential oil (droplet size of 196 ± 8 nm), it was gelified.					
36207726	0	18	theme	oil	108:110	arg1	nanofibers					128:137	Mentha spicata L. essential oil and electrospun nanofibers	80:137	Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose	80:187	Antioxidative, anticancer, and antibacterial activities of a nanogel containing Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose.					
36207726	12	19	with	mat	1829:1831	arg1	effects					1882:1888	no cytotoxic, antioxidant, or antibacterial effects	1838:1888	no cytotoxic, antioxidant, or antibacterial effects	1838:1888	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	12	20	theme	prepared	1750:1757	arg1	mat					1829:1831	the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat	1746:1831	the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects	1746:1888	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	12	20	theme	prepared	1750:1757	arg1	dressing					1913:1920	lesion dressing	1906:1920	lesion dressing after treatment with the nanogel	1906:1953	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	8	21	theme	common	1211:1216	arg1	model					1273:1277	the Carreau-Yasuda model	1254:1277	the Carreau-Yasuda model	1254:1277	The viscosity was fully fitted with a common non-Newtonian viscosity regression, the Carreau-Yasuda model.					
36207726	8	21	theme	common	1211:1216	arg1	regression					1242:1251	a common non-Newtonian viscosity regression	1209:1251	a common non-Newtonian viscosity regression	1209:1251	The viscosity was fully fitted with a common non-Newtonian viscosity regression, the Carreau-Yasuda model.					
36207726	10	22	theme	55.0 µg/mL	1495:1504	arg1	value					1486:1490	an IC50 value	1478:1490	an IC50 value of 55.0 µg/mL	1478:1504	Furthermore, the potency of the nanogel with an IC50 value of 55.0 µg/mL was substantially more (P < 0.001) than the essential oil (997.4 µg/mL).					
36207726	2	23	theme	natural	390:396	arg1	agents					492:497	antibacterial agents	478:497	antibacterial agents	478:497	Recently, many attempts have been made to develop natural nanogels that, besides hydrating the skin, could also be used as antioxidant or antibacterial agents.					
36207726	2	23	theme	natural	390:396	arg1	antioxidant					463:473	antioxidant	463:473	antioxidant	463:473	Recently, many attempts have been made to develop natural nanogels that, besides hydrating the skin, could also be used as antioxidant or antibacterial agents.					
36207726	2	23	theme	natural	390:396	arg1	nanogels					398:405	natural nanogels	390:405	natural nanogels that, besides hydrating the skin, could also be used as antioxidant or antibacterial agents	390:497	Recently, many attempts have been made to develop natural nanogels that, besides hydrating the skin, could also be used as antioxidant or antibacterial agents.					
36207726	0	24	theme	essential	98:106	arg1	oil					108:110	essential oil	98:110	essential oil	98:110	Antioxidative, anticancer, and antibacterial activities of a nanogel containing Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose.					
36207726	12	25	theme	nanofibers	1818:1827	arg1	mat					1829:1831	the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat	1746:1831	the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects	1746:1888	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	12	25	theme	nanofibers	1818:1827	arg1	dressing					1913:1920	lesion dressing	1906:1920	lesion dressing after treatment with the nanogel	1906:1953	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	4	26	theme	FTIR	758:761	arg1	analysis					763:770	FTIR analysis	758:770	FTIR analysis	758:770	Its nanoemulsion-based nanogel was then investigated; successful loading of the essential oil in the nanogel was confirmed using FTIR analysis.					
36207726	4	27	theme	nanoemulsion-based	633:650	arg1	nanogel					652:658	Its nanoemulsion-based nanogel	629:658	Its nanoemulsion-based nanogel	629:658	Its nanoemulsion-based nanogel was then investigated; successful loading of the essential oil in the nanogel was confirmed using FTIR analysis.					
36207726	7	28	theme	%	1062:1062	arg1	carboxymethylcellulose					1035:1056	carboxymethylcellulose	1035:1056	carboxymethylcellulose (3.5% w/v)	1035:1067	By adding carboxymethylcellulose (3.5% w/v) to the optimal nanoemulsion containing the essential oil (droplet size of 196 ± 8 nm), it was gelified.					
36207726	7	28	theme	%	1062:1062	arg1	w/v					1064:1066	3.5% w/v	1059:1066	3.5% w/v	1059:1066	By adding carboxymethylcellulose (3.5% w/v) to the optimal nanoemulsion containing the essential oil (droplet size of 196 ± 8 nm), it was gelified.					
36207726	11	29	theme	coli	1637:1640	arg1	growth					1589:1594	the growth	1585:1594	the growth of Staphylococcus aureus and Escherichia coli after treatment with 1000 µg/mL nanogel	1585:1680	Also, the growth of Staphylococcus aureus and Escherichia coli after treatment with 1000 µg/mL nanogel was about 50% decreased compared to the control group.					
36207726	0	30	theme	nanogel	61:67	arg1	Antioxidative					0:12	Antioxidative	0:12	Antioxidative	0:12	Antioxidative, anticancer, and antibacterial activities of a nanogel containing Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose.					
36207726	7	31	dep	oil	1122:1124	arg1	size					1135:1138	droplet size	1127:1138	droplet size of 196 ± 8 nm	1127:1152	By adding carboxymethylcellulose (3.5% w/v) to the optimal nanoemulsion containing the essential oil (droplet size of 196 ± 8 nm), it was gelified.					
36207726	1	32	theme	largest	208:214	arg1	organ					216:220	the largest organ	204:220	the largest organ	204:220	BACKGROUND As the largest organ, the skin has been frequently affected by trauma, chemical materials, toxins, bacterial pathogens, and free radicals.					
36207726	1	32	theme	largest	208:214	arg1	skin					227:230	the skin	223:230	the skin	223:230	BACKGROUND As the largest organ, the skin has been frequently affected by trauma, chemical materials, toxins, bacterial pathogens, and free radicals.					
36207726	1	33	dep	BACKGROUND	190:199	arg1	affected					252:259	affected	252:259	has been frequently affected by trauma, chemical materials, toxins, bacterial pathogens, and free radicals	232:337	BACKGROUND As the largest organ, the skin has been frequently affected by trauma, chemical materials, toxins, bacterial pathogens, and free radicals.					
36207726	14	34	theme	vivo	2126:2129	arg1	studies					2131:2137	vivo studies	2126:2137	vivo studies	2126:2137	Therefore, it could be considered for further consideration in vivo studies.					
36207726	12	35	theme	antioxidant	1852:1862	arg1	effects					1882:1888	no cytotoxic, antioxidant, or antibacterial effects	1838:1888	no cytotoxic, antioxidant, or antibacterial effects	1838:1888	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	0	36	theme	electrospun	116:126	arg1	nanofibers					128:137	Mentha spicata L. essential oil and electrospun nanofibers	80:137	Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose	80:187	Antioxidative, anticancer, and antibacterial activities of a nanogel containing Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose.					
36207726	11	37	with	treatment	1648:1656	arg1	nanogel					1674:1680	1000 µg/mL nanogel	1663:1680	1000 µg/mL nanogel	1663:1680	Also, the growth of Staphylococcus aureus and Escherichia coli after treatment with 1000 µg/mL nanogel was about 50% decreased compared to the control group.					
36207726	3	38	theme	chemical	527:534	arg1	composition					536:546	the chemical composition	523:546	the chemical composition of the Mentha spicata essential oil	523:582	METHODS In this study, the chemical composition of the Mentha spicata essential oil was first investigated using GC-MS analysis.					
36207726	8	39	theme	Carreau-Yasuda	1258:1271	arg1	model					1273:1277	the Carreau-Yasuda model	1254:1277	the Carreau-Yasuda model	1254:1277	The viscosity was fully fitted with a common non-Newtonian viscosity regression, the Carreau-Yasuda model.					
36207726	8	39	theme	Carreau-Yasuda	1258:1271	arg1	regression					1242:1251	a common non-Newtonian viscosity regression	1209:1251	a common non-Newtonian viscosity regression	1209:1251	The viscosity was fully fitted with a common non-Newtonian viscosity regression, the Carreau-Yasuda model.					
36207726	8	40	theme	non-Newtonian	1218:1230	arg1	model					1273:1277	the Carreau-Yasuda model	1254:1277	the Carreau-Yasuda model	1254:1277	The viscosity was fully fitted with a common non-Newtonian viscosity regression, the Carreau-Yasuda model.					
36207726	8	40	theme	non-Newtonian	1218:1230	arg1	regression					1242:1251	a common non-Newtonian viscosity regression	1209:1251	a common non-Newtonian viscosity regression	1209:1251	The viscosity was fully fitted with a common non-Newtonian viscosity regression, the Carreau-Yasuda model.					
36207726	8	41	theme	viscosity	1232:1240	arg1	model					1273:1277	the Carreau-Yasuda model	1254:1277	the Carreau-Yasuda model	1254:1277	The viscosity was fully fitted with a common non-Newtonian viscosity regression, the Carreau-Yasuda model.					
36207726	8	41	theme	viscosity	1232:1240	arg1	regression					1242:1251	a common non-Newtonian viscosity regression	1209:1251	a common non-Newtonian viscosity regression	1209:1251	The viscosity was fully fitted with a common non-Newtonian viscosity regression, the Carreau-Yasuda model.					
36207726	9	42	theme	examined	1390:1397	arg1	62.5-1000 µg/mL					1415:1429	62.5-1000 µg/mL	1415:1429	62.5-1000 µg/mL	1415:1429	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	9	42	theme	examined	1390:1397	arg1	concentrations					1399:1412	all examined concentrations	1386:1412	all examined concentrations (62.5-1000 µg/mL)	1386:1430	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	0	43	theme	antibacterial	31:43	arg1	activities					45:54	antibacterial activities	31:54	antibacterial activities	31:54	Antioxidative, anticancer, and antibacterial activities of a nanogel containing Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose.					
36207726	1	44	theme	bacterial	300:308	arg1	pathogens					310:318	bacterial pathogens	300:318	bacterial pathogens	300:318	BACKGROUND As the largest organ, the skin has been frequently affected by trauma, chemical materials, toxins, bacterial pathogens, and free radicals.					
36207726	3	45	theme	essential	570:578	arg1	oil					580:582	the Mentha spicata essential oil	551:582	the Mentha spicata essential oil	551:582	METHODS In this study, the chemical composition of the Mentha spicata essential oil was first investigated using GC-MS analysis.					
36207726	11	46	theme	control	1722:1728	arg1	group					1730:1734	the control group	1718:1734	the control group	1718:1734	Also, the growth of Staphylococcus aureus and Escherichia coli after treatment with 1000 µg/mL nanogel was about 50% decreased compared to the control group.					
36207726	5	47	theme	antibacterial	823:835	arg1	activities					837:846	antibacterial activities	823:846	antibacterial activities	823:846	Besides, nanogel's antioxidative, anticancer, and antibacterial activities were investigated.					
36207726	10	48	theme	IC50	1481:1484	arg1	value					1486:1490	an IC50 value	1478:1490	an IC50 value of 55.0 µg/mL	1478:1504	Furthermore, the potency of the nanogel with an IC50 value of 55.0 µg/mL was substantially more (P < 0.001) than the essential oil (997.4 µg/mL).					
36207726	12	49	theme	methylcellulose	1802:1816	arg1	mat					1829:1831	the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat	1746:1831	the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects	1746:1888	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	12	49	theme	methylcellulose	1802:1816	arg1	dressing					1913:1920	lesion dressing	1906:1920	lesion dressing after treatment with the nanogel	1906:1953	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	3	50	theme	GC-MS	613:617	arg1	analysis					619:626	GC-MS analysis	613:626	GC-MS analysis	613:626	METHODS In this study, the chemical composition of the Mentha spicata essential oil was first investigated using GC-MS analysis.					
36207726	3	51	theme	oil	580:582	arg1	composition					536:546	the chemical composition	523:546	the chemical composition of the Mentha spicata essential oil	523:582	METHODS In this study, the chemical composition of the Mentha spicata essential oil was first investigated using GC-MS analysis.					
36207726	11	52	theme	1000 µg/mL	1663:1672	arg1	nanogel					1674:1680	1000 µg/mL nanogel	1663:1680	1000 µg/mL nanogel	1663:1680	Also, the growth of Staphylococcus aureus and Escherichia coli after treatment with 1000 µg/mL nanogel was about 50% decreased compared to the control group.					
36207726	12	53	with	treatment	1928:1936	arg1	nanogel					1947:1953	the nanogel	1943:1953	the nanogel	1943:1953	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	0	54	theme	methylcellulose	173:187	arg1	nanofibers					128:137	Mentha spicata L. essential oil and electrospun nanofibers	80:137	Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose	80:187	Antioxidative, anticancer, and antibacterial activities of a nanogel containing Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose.					
36207726	14	55	theme	further	2101:2107	arg1	consideration					2109:2121	further consideration	2101:2121	further consideration in vivo studies	2101:2137	Therefore, it could be considered for further consideration in vivo studies.					
36207726	3	56	theme	Mentha	555:560	arg1	oil					580:582	the Mentha spicata essential oil	551:582	the Mentha spicata essential oil	551:582	METHODS In this study, the chemical composition of the Mentha spicata essential oil was first investigated using GC-MS analysis.					
36207726	9	57	theme	essential	1357:1365	arg1	oil					1367:1369	the essential oil	1353:1369	the essential oil	1353:1369	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	13	58	theme	High	1956:1959	arg1	potency					1961:1967	High potency	1956:1967	High potency	1956:1967	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	13	58	theme	High	1956:1959	arg1	ingredients					1978:1988	natural ingredients	1970:1988	natural ingredients	1970:1988	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	13	58	theme	High	1956:1959	arg1	advantages					2027:2036	advantages	2027:2036	advantages of the prepared nanogel	2027:2060	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	13	58	theme	High	1956:1959	arg1	preparation					2011:2021	straightforward preparation	1995:2021	straightforward preparation	1995:2021	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	6	59	dep	RESULTS	867:873	arg1	β-pinene					926:933	β-pinene	926:933	β-pinene (3.3%)	926:940	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	6	59	dep	RESULTS	867:873	arg1	limonene					892:899	limonene	892:899	limonene (28.5%)	892:907	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	6	59	dep	RESULTS	867:873	arg1	pulegone					947:954	pulegone	947:954	pulegone (3.3%)	947:961	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	6	59	dep	RESULTS	867:873	arg1	borneol					910:916	borneol	910:916	borneol (3.9%)	910:923	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	6	59	dep	RESULTS	867:873	arg1	Carvone					875:881	Carvone	875:881	Carvone (37.1%)	875:889	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	6	59	dep	RESULTS	867:873	arg1	%					922:922	3.9%	919:922	3.9%	919:922	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	6	59	dep	RESULTS	867:873	arg1	%					939:939	3.3%	936:939	3.3%	936:939	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	6	59	dep	RESULTS	867:873	arg1	%					888:888	37.1%	884:888	37.1%	884:888	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	6	59	dep	RESULTS	867:873	arg1	%					906:906	28.5%	902:906	28.5%	902:906	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	6	59	dep	RESULTS	867:873	arg1	%					960:960	3.3%	957:960	3.3%	957:960	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	6	59	dep	RESULTS	867:873	arg1	RESULTS					867:873	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%)	867:961	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%)	867:961	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	0	60	theme	polycaprolactone-hydroxypropyl	142:171	arg1	methylcellulose					173:187	polycaprolactone-hydroxypropyl methylcellulose	142:187	polycaprolactone-hydroxypropyl methylcellulose	142:187	Antioxidative, anticancer, and antibacterial activities of a nanogel containing Mentha spicata L. essential oil and electrospun nanofibers of polycaprolactone-hydroxypropyl methylcellulose.					
36207726	3	61	theme	spicata	562:568	arg1	oil					580:582	the Mentha spicata essential oil	551:582	the Mentha spicata essential oil	551:582	METHODS In this study, the chemical composition of the Mentha spicata essential oil was first investigated using GC-MS analysis.					
36207726	10	62	with	nanogel	1465:1471	arg1	value					1486:1490	an IC50 value	1478:1490	an IC50 value of 55.0 µg/mL	1478:1504	Furthermore, the potency of the nanogel with an IC50 value of 55.0 µg/mL was substantially more (P < 0.001) than the essential oil (997.4 µg/mL).					
36207726	6	63	theme	essential	1010:1018	arg1	oil					1020:1022	the essential oil	1006:1022	the essential oil	1006:1022	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	1	64	theme	free	325:328	arg1	radicals					330:337	free radicals	325:337	free radicals	325:337	BACKGROUND As the largest organ, the skin has been frequently affected by trauma, chemical materials, toxins, bacterial pathogens, and free radicals.					
36207726	10	65	dep	more	1524:1527	arg1	P < 0.001					1530:1538	P < 0.001	1530:1538	P < 0.001	1530:1538	Furthermore, the potency of the nanogel with an IC50 value of 55.0 µg/mL was substantially more (P < 0.001) than the essential oil (997.4 µg/mL).					
36207726	4	66	theme	oil	719:721	arg1	loading					694:700	successful loading	683:700	successful loading of the essential oil in the nanogel	683:736	Its nanoemulsion-based nanogel was then investigated; successful loading of the essential oil in the nanogel was confirmed using FTIR analysis.					
36207726	3	67	dep	METHODS	500:506	arg1	study					516:520	this study	511:520	this study	511:520	METHODS In this study, the chemical composition of the Mentha spicata essential oil was first investigated using GC-MS analysis.					
36207726	13	68	theme	prepared	2045:2052	arg1	nanogel					2054:2060	the prepared nanogel	2041:2060	the prepared nanogel	2041:2060	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	11	69	dep	decreased	1696:1704	arg1	%					1694:1694	about 50%	1686:1694	about 50%	1686:1694	Also, the growth of Staphylococcus aureus and Escherichia coli after treatment with 1000 µg/mL nanogel was about 50% decreased compared to the control group.					
36207726	12	70	theme	antibacterial	1868:1880	arg1	effects					1882:1888	no cytotoxic, antioxidant, or antibacterial effects	1838:1888	no cytotoxic, antioxidant, or antibacterial effects	1838:1888	Besides, the prepared electrospun polycaprolactone-hydroxypropyl methylcellulose nanofibers mat with no cytotoxic, antioxidant, or antibacterial effects was proposed as lesion dressing after treatment with the nanogel.					
36207726	7	71	contain	containing	1097:1106	arg2	oil					1122:1124	the essential oil	1108:1124	the essential oil (droplet size of 196 ± 8 nm)	1108:1153	By adding carboxymethylcellulose (3.5% w/v) to the optimal nanoemulsion containing the essential oil (droplet size of 196 ± 8 nm), it was gelified.					
36207726	7	71	contain	containing	1097:1106	arg1	nanoemulsion					1084:1095	the optimal nanoemulsion	1072:1095	the optimal nanoemulsion containing the essential oil (droplet size of 196 ± 8 nm)	1072:1153	By adding carboxymethylcellulose (3.5% w/v) to the optimal nanoemulsion containing the essential oil (droplet size of 196 ± 8 nm), it was gelified.					
36207726	4	72	theme	essential	709:717	arg1	oil					719:721	the essential oil	705:721	the essential oil	705:721	Its nanoemulsion-based nanogel was then investigated; successful loading of the essential oil in the nanogel was confirmed using FTIR analysis.					
36207726	13	73	theme	nanogel	2054:2060	arg1	potency					1961:1967	High potency	1956:1967	High potency	1956:1967	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	13	73	theme	nanogel	2054:2060	arg1	ingredients					1978:1988	natural ingredients	1970:1988	natural ingredients	1970:1988	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	13	73	theme	nanogel	2054:2060	arg1	advantages					2027:2036	advantages	2027:2036	advantages of the prepared nanogel	2027:2060	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	13	73	theme	nanogel	2054:2060	arg1	preparation					2011:2021	straightforward preparation	1995:2021	straightforward preparation	1995:2021	High potency, natural ingredients, and straightforward preparation are advantages of the prepared nanogel.					
36207726	2	74	theme	antibacterial	478:490	arg1	nanogels					398:405	natural nanogels	390:405	natural nanogels that, besides hydrating the skin, could also be used as antioxidant or antibacterial agents	390:497	Recently, many attempts have been made to develop natural nanogels that, besides hydrating the skin, could also be used as antioxidant or antibacterial agents.					
36207726	2	74	theme	antibacterial	478:490	arg1	agents					492:497	antibacterial agents	478:497	antibacterial agents	478:497	Recently, many attempts have been made to develop natural nanogels that, besides hydrating the skin, could also be used as antioxidant or antibacterial agents.					
36207726	10	75	theme	essential	1550:1558	arg1	997.4 µg/mL					1565:1575	997.4 µg/mL	1565:1575	997.4 µg/mL	1565:1575	Furthermore, the potency of the nanogel with an IC50 value of 55.0 µg/mL was substantially more (P < 0.001) than the essential oil (997.4 µg/mL).					
36207726	10	75	theme	essential	1550:1558	arg1	oil					1560:1562	the essential oil	1546:1562	the essential oil (997.4 µg/mL)	1546:1576	Furthermore, the potency of the nanogel with an IC50 value of 55.0 µg/mL was substantially more (P < 0.001) than the essential oil (997.4 µg/mL).					
36207726	7	76	theme	droplet	1127:1133	arg1	size					1135:1138	droplet size	1127:1138	droplet size of 196 ± 8 nm	1127:1152	By adding carboxymethylcellulose (3.5% w/v) to the optimal nanoemulsion containing the essential oil (droplet size of 196 ± 8 nm), it was gelified.					
36207726	9	77	theme	nanogel	1310:1316	arg1	potent					1341:1346	potent	1341:1346	potent	1341:1346	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	9	77	theme	nanogel	1310:1316	arg1	P < 0.001					1372:1380	P < 0.001	1372:1380	P < 0.001	1372:1380	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	9	77	theme	nanogel	1310:1316	arg1	effect					1296:1301	The antioxidant effect	1280:1301	The antioxidant effect of the nanogel	1280:1316	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	14	78	from	consideration	2109:2121	arg1	studies					2131:2137	vivo studies	2126:2137	vivo studies	2126:2137	Therefore, it could be considered for further consideration in vivo studies.					
36207726	7	79	theme	essential	1112:1120	arg1	oil					1122:1124	the essential oil	1108:1124	the essential oil (droplet size of 196 ± 8 nm)	1108:1153	By adding carboxymethylcellulose (3.5% w/v) to the optimal nanoemulsion containing the essential oil (droplet size of 196 ± 8 nm), it was gelified.					
36207726	9	80	dep	potent	1341:1346	arg1	potent					1341:1346	potent	1341:1346	potent	1341:1346	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	9	80	dep	potent	1341:1346	arg1	P < 0.001					1372:1380	P < 0.001	1372:1380	P < 0.001	1372:1380	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	9	80	dep	potent	1341:1346	arg1	effect					1296:1301	The antioxidant effect	1280:1301	The antioxidant effect of the nanogel	1280:1316	The antioxidant effect of the nanogel was significantly more potent than the essential oil (P < 0.001) at all examined concentrations (62.5-1000 µg/mL).					
36207726	6	81	from	compounds	993:1001	arg1	oil					1020:1022	the essential oil	1006:1022	the essential oil	1006:1022	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	6	82	theme	major	987:991	arg1	compounds					993:1001	five major compounds	982:1001	five major compounds in the essential oil	982:1022	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	6	82	theme	major	987:991	arg1	RESULTS					867:873	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%)	867:961	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%)	867:961	RESULTS Carvone (37.1%), limonene (28.5%), borneol (3.9%), β-pinene (3.3%), and pulegone (3.3%) were identified as five major compounds in the essential oil.					
36207726	4	83	from	loading	694:700	arg1	nanogel					730:736	the nanogel	726:736	the nanogel	726:736	Its nanoemulsion-based nanogel was then investigated; successful loading of the essential oil in the nanogel was confirmed using FTIR analysis.					
36207726	7	84	theme	optimal	1076:1082	arg1	nanoemulsion					1084:1095	the optimal nanoemulsion	1072:1095	the optimal nanoemulsion containing the essential oil (droplet size of 196 ± 8 nm)	1072:1153	By adding carboxymethylcellulose (3.5% w/v) to the optimal nanoemulsion containing the essential oil (droplet size of 196 ± 8 nm), it was gelified.					
37115254	4	0	theme	bacterial	614:622	arg1	yield					634:638	bacterial cellulose yield	614:638	bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed)	614:702	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	13	1	theme	bacterial	2036:2044	arg1	cellulose					2046:2054	bacterial cellulose	2036:2054	bacterial cellulose	2036:2054	• Komagataeibacter strains support the agro-waste conversion in bacterial cellulose.					
37115254	2	2	theme	liquid	341:346	arg1	chromatography					348:361	high-pressure liquid chromatography	327:361	high-pressure liquid chromatography	327:361	Organic acids and phenolic compounds composition were assayed by high-pressure liquid chromatography.					
37115254	1	3	theme	acetic	232:237	arg1	bacteria					244:251	acetic acid bacteria	232:251	acetic acid bacteria	232:251	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	4	4	theme	yield	634:638	arg1	terms					605:609	terms	605:609	terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed)	605:702	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	10	5	theme	whey	1637:1640	arg1	cheese					1630:1635	cheese whey	1630:1640	cheese whey	1630:1640	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	5	6	theme	Bacterial	705:713	arg1	cellulose					715:723	Bacterial cellulose	705:723	Bacterial cellulose produced in olive mill wastewater	705:757	Bacterial cellulose produced in olive mill wastewater presented a more well-defined network compared to pellicles produced in cheese whey, resulting in a smaller fiber diameter in most cases.					
37115254	2	7	theme	Organic	262:268	arg1	acids					270:274	Organic acids	262:274	Organic acids	262:274	Organic acids and phenolic compounds composition were assayed by high-pressure liquid chromatography.					
37115254	6	8	theme	chemical	933:940	arg1	structure					942:950	bacterial cellulose chemical structure	913:950	bacterial cellulose chemical structure	913:950	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	8	9	theme	rRNA	1221:1224	arg1	sequencing					1231:1240	16S rRNA gene sequencing	1217:1240	16S rRNA gene sequencing	1217:1240	The acetic acid bacteria strains used in this study were characterized by 16S rRNA gene sequencing, allowing to assign them to Komagataeibacter xylinus and Komagataeibacter rhaeticus species.					
37115254	6	10	theme	bacterial	913:921	arg1	structure					942:950	bacterial cellulose chemical structure	913:950	bacterial cellulose chemical structure	913:950	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	2	11	theme	compounds	289:297	arg1	composition					299:309	Organic acids and phenolic compounds composition	262:309	Organic acids and phenolic compounds composition	262:309	Organic acids and phenolic compounds composition were assayed by high-pressure liquid chromatography.					
37115254	9	12	theme	acid	1531:1534	arg1	bacteria					1536:1543	acetic acid bacteria	1524:1543	acetic acid bacteria	1524:1543	This study proves the suitability to perform sustainable bioprocesses for producing bacterial cellulose, combining the valorisation of agro-wastes with microbial conversions carried out by acetic acid bacteria.					
37115254	10	13	theme	olive	1646:1650	arg1	wastewater					1657:1666	olive mill wastewater	1646:1666	olive mill wastewater	1646:1666	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	4	14	dep	yield	634:638	arg1	0.300 g					641:647	0.300 g	641:647	0.300 g of bacterial cellulose/gram of carbon source consumed	641:701	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	10	15	theme	bacterial	1783:1791	arg1	cellulose					1793:1801	the bacterial cellulose	1779:1801	the bacterial cellulose	1779:1801	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	1	16	theme	potential	168:176	arg1	whey					116:119	cheese whey and olive mill wastewater	109:145	whey	116:119	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	1	16	theme	potential	168:176	arg1	feedstocks					178:187	potential feedstocks	168:187	potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains	168:259	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	1	16	theme	potential	168:176	arg1	wastewater					136:145	cheese whey and olive mill wastewater	109:145	wastewater	136:145	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	4	17	theme	source	687:692	arg1	cellulose/gram					662:675	bacterial cellulose/gram	652:675	bacterial cellulose/gram of carbon source consumed	652:701	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	7	18	dep	80.82	1135:1139	arg1	to					1132:1133	to	1132:1133	to	1132:1133	The crystallinity ranged from 45.72 to 80.82%.					
37115254	11	19	theme	cellulose	1882:1890	arg1	production					1892:1901	bacterial cellulose production	1872:1901	bacterial cellulose production	1872:1901	KEY POINTS: • Cheese whey and olive mill wastewater can be used for bacterial cellulose production.					
37115254	9	20	theme	microbial	1487:1495	arg1	conversions					1497:1507	microbial conversions	1487:1507	microbial conversions carried out by acetic acid bacteria	1487:1543	This study proves the suitability to perform sustainable bioprocesses for producing bacterial cellulose, combining the valorisation of agro-wastes with microbial conversions carried out by acetic acid bacteria.					
37115254	4	21	from	feedstock	592:600	arg1	terms					605:609	terms	605:609	terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed)	605:702	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	6	22	theme	bonds	999:1003	arg1	presence					968:975	the presence	964:975	the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components	964:1093	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	0	23	from	whey	49:52	arg1	bacteria					12:19	Acetic acid bacteria	0:19	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.	0:92	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.					
37115254	0	23	from	whey	49:52	arg1	agro-wastes					24:34	agro-wastes:	24:35	agro-wastes: from cheese whey and olive mill wastewater to cellulose	24:91	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.					
37115254	5	24	from	diameter	873:880	arg1	cases					890:894	most cases	885:894	most cases	885:894	Bacterial cellulose produced in olive mill wastewater presented a more well-defined network compared to pellicles produced in cheese whey, resulting in a smaller fiber diameter in most cases.					
37115254	6	25	theme	different	980:988	arg1	bonds					999:1003	different chemical bonds	980:1003	different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components	980:1093	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	13	26	theme	Komagataeibacter	1974:1989	arg1	strains					1991:1997	• Komagataeibacter strains	1972:1997	• Komagataeibacter strains	1972:1997	• Komagataeibacter strains support the agro-waste conversion in bacterial cellulose.					
37115254	11	27	theme	•	1816:1816	arg1	whey					1825:1828	• Cheese whey	1816:1828	• Cheese whey	1816:1828	KEY POINTS: • Cheese whey and olive mill wastewater can be used for bacterial cellulose production.					
37115254	5	28	theme	olive	737:741	arg1	wastewater					748:757	olive mill wastewater	737:757	olive mill wastewater	737:757	Bacterial cellulose produced in olive mill wastewater presented a more well-defined network compared to pellicles produced in cheese whey, resulting in a smaller fiber diameter in most cases.					
37115254	10	29	from	versatility	1555:1565	arg1	terms					1570:1574	terms	1570:1574	terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater	1570:1666	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	0	30	from	wastewater	69:78	arg1	bacteria					12:19	Acetic acid bacteria	0:19	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.	0:92	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.					
37115254	0	30	from	wastewater	69:78	arg1	agro-wastes					24:34	agro-wastes:	24:35	agro-wastes: from cheese whey and olive mill wastewater to cellulose	24:91	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.					
37115254	6	31	theme	mill	1052:1055	arg1	wastewater					1057:1066	olive mill wastewater	1046:1066	olive mill wastewater	1046:1066	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	0	32	from	bacteria	12:19	arg1	whey					49:52	cheese whey and olive mill wastewater	42:78	whey	49:52	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.					
37115254	0	32	from	bacteria	12:19	arg1	wastewater					69:78	cheese whey and olive mill wastewater	42:78	wastewater	69:78	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.					
37115254	0	32	from	bacteria	12:19	arg1	agro-wastes					24:34	agro-wastes:	24:35	agro-wastes: from cheese whey and olive mill wastewater to cellulose	24:91	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.					
37115254	11	33	theme	olive	1834:1838	arg1	wastewater					1845:1854	olive mill wastewater	1834:1854	olive mill wastewater	1834:1854	KEY POINTS: • Cheese whey and olive mill wastewater can be used for bacterial cellulose production.					
37115254	0	34	theme	olive	58:62	arg1	wastewater					69:78	cheese whey and olive mill wastewater	42:78	wastewater	69:78	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.					
37115254	1	35	theme	olive	125:129	arg1	whey					116:119	cheese whey and olive mill wastewater	109:145	whey	116:119	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	1	35	theme	olive	125:129	arg1	feedstocks					178:187	potential feedstocks	168:187	potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains	168:259	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	1	35	theme	olive	125:129	arg1	wastewater					136:145	cheese whey and olive mill wastewater	109:145	wastewater	136:145	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	5	36	theme	well-defined	776:787	arg1	network					789:795	a more well-defined network	769:795	a more well-defined network	769:795	Bacterial cellulose produced in olive mill wastewater presented a more well-defined network compared to pellicles produced in cheese whey, resulting in a smaller fiber diameter in most cases.					
37115254	10	37	theme	high	1550:1553	arg1	versatility					1555:1565	The high versatility	1546:1565	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater	1546:1666	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	3	38	used	used	462:465	arg2	microscopy					423:432	scanning electron microscopy	405:432	scanning electron microscopy	405:432	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	3	38	used	used	462:465	arg2	diffraction					445:455	X-ray diffraction	439:455	X-ray diffraction	439:455	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	3	38	used	used	462:465	arg2	spectroscopy					391:402	Fourier-transform infrared spectroscopy	364:402	Fourier-transform infrared spectroscopy	364:402	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	10	39	theme	customized	1725:1734	arg1	bioprocesses					1736:1747	customized bioprocesses	1725:1747	customized bioprocesses depending on the final use of the bacterial cellulose	1725:1801	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	3	40	theme	X-ray	439:443	arg1	diffraction					445:455	X-ray diffraction	439:455	X-ray diffraction	439:455	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	0	41	theme	Acetic	0:5	arg1	bacteria					12:19	Acetic acid bacteria	0:19	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.	0:92	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.					
37115254	9	42	theme	sustainable	1380:1390	arg1	bioprocesses					1392:1403	sustainable bioprocesses	1380:1403	sustainable bioprocesses	1380:1403	This study proves the suitability to perform sustainable bioprocesses for producing bacterial cellulose, combining the valorisation of agro-wastes with microbial conversions carried out by acetic acid bacteria.					
37115254	8	43	dep	Komagataeibacter	1270:1285	arg1	xylinus					1287:1293	Komagataeibacter xylinus	1270:1293	Komagataeibacter xylinus	1270:1293	The acetic acid bacteria strains used in this study were characterized by 16S rRNA gene sequencing, allowing to assign them to Komagataeibacter xylinus and Komagataeibacter rhaeticus species.					
37115254	8	43	dep	Komagataeibacter	1270:1285	arg1	species					1326:1332	species	1326:1332	species	1326:1332	The acetic acid bacteria strains used in this study were characterized by 16S rRNA gene sequencing, allowing to assign them to Komagataeibacter xylinus and Komagataeibacter rhaeticus species.					
37115254	3	44	theme	infrared	382:389	arg1	spectroscopy					391:402	Fourier-transform infrared spectroscopy	364:402	Fourier-transform infrared spectroscopy	364:402	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	4	45	theme	efficient	582:590	arg1	feedstock					592:600	the most efficient feedstock	573:600	the most efficient feedstock	573:600	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	4	45	theme	efficient	582:590	arg1	whey					564:567	Cheese whey	557:567	Cheese whey	557:567	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	5	46	theme	fiber	867:871	arg1	diameter					873:880	a smaller fiber diameter	857:880	a smaller fiber diameter in most cases	857:894	Bacterial cellulose produced in olive mill wastewater presented a more well-defined network compared to pellicles produced in cheese whey, resulting in a smaller fiber diameter in most cases.					
37115254	5	47	theme	whey	838:841	arg1	cheese					831:836	cheese whey	831:841	cheese whey	831:841	Bacterial cellulose produced in olive mill wastewater presented a more well-defined network compared to pellicles produced in cheese whey, resulting in a smaller fiber diameter in most cases.					
37115254	9	48	theme	bacterial	1419:1427	arg1	cellulose					1429:1437	bacterial cellulose	1419:1437	bacterial cellulose	1419:1437	This study proves the suitability to perform sustainable bioprocesses for producing bacterial cellulose, combining the valorisation of agro-wastes with microbial conversions carried out by acetic acid bacteria.					
37115254	6	49	theme	whey	1079:1082	arg1	components					1084:1093	cheese whey components	1072:1093	cheese whey components	1072:1093	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	3	50	theme	electron	414:421	arg1	microscopy					423:432	scanning electron microscopy	405:432	scanning electron microscopy	405:432	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	3	51	theme	chemical	519:526	arg1	structure					546:554	bacterial cellulose chemical and morphological structure	499:554	bacterial cellulose chemical and morphological structure	499:554	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	11	52	theme	KEY	1804:1806	arg1	POINTS					1808:1813	KEY POINTS	1804:1813	KEY POINTS: • Cheese whey and olive mill wastewater can be used for bacterial cellulose production.	1804:1902	KEY POINTS: • Cheese whey and olive mill wastewater can be used for bacterial cellulose production.					
37115254	3	53	theme	bacterial	499:507	arg1	structure					546:554	bacterial cellulose chemical and morphological structure	499:554	bacterial cellulose chemical and morphological structure	499:554	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	8	54	theme	acid	1154:1157	arg1	bacteria					1159:1166	The acetic acid bacteria	1143:1166	The acetic acid bacteria strains used in this study	1143:1193	The acetic acid bacteria strains used in this study were characterized by 16S rRNA gene sequencing, allowing to assign them to Komagataeibacter xylinus and Komagataeibacter rhaeticus species.					
37115254	1	55	theme	cellulose	213:221	arg1	strains					253:259	bacterial cellulose by using acetic acid bacteria strains	203:259	bacterial cellulose by using acetic acid bacteria strains	203:259	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	10	56	theme	fiber	1602:1606	arg1	diameters					1608:1616	fiber diameters	1602:1616	fiber diameters	1602:1616	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	3	57	theme	morphological	532:544	arg1	structure					546:554	bacterial cellulose chemical and morphological structure	499:554	bacterial cellulose chemical and morphological structure	499:554	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	12	58	theme	•	1904:1904	arg1	structure					1926:1934	• Bacterial cellulose structure	1904:1934	• Bacterial cellulose structure	1904:1934	• Bacterial cellulose structure is dependent on the culture medium.					
37115254	2	59	theme	acids	270:274	arg1	composition					299:309	Organic acids and phenolic compounds composition	262:309	Organic acids and phenolic compounds composition	262:309	Organic acids and phenolic compounds composition were assayed by high-pressure liquid chromatography.					
37115254	1	60	theme	acid	239:242	arg1	bacteria					244:251	acetic acid bacteria	232:251	acetic acid bacteria	232:251	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	6	61	theme	structure	942:950	arg1	analysis					901:908	The analysis	897:908	The analysis of bacterial cellulose chemical structure	897:950	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	4	62	theme	cellulose	624:632	arg1	yield					634:638	bacterial cellulose yield	614:638	bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed)	614:702	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	2	63	theme	high-pressure	327:339	arg1	chromatography					348:361	high-pressure liquid chromatography	327:361	high-pressure liquid chromatography	327:361	Organic acids and phenolic compounds composition were assayed by high-pressure liquid chromatography.					
37115254	1	64	theme	mill	131:134	arg1	whey					116:119	cheese whey and olive mill wastewater	109:145	whey	116:119	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	1	64	theme	mill	131:134	arg1	feedstocks					178:187	potential feedstocks	168:187	potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains	168:259	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	1	64	theme	mill	131:134	arg1	wastewater					136:145	cheese whey and olive mill wastewater	109:145	wastewater	136:145	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	6	65	theme	cellulose	923:931	arg1	structure					942:950	bacterial cellulose chemical structure	913:950	bacterial cellulose chemical structure	913:950	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	13	66	theme	agro-waste	2011:2020	arg1	conversion					2022:2031	the agro-waste conversion	2007:2031	the agro-waste conversion in bacterial cellulose	2007:2054	• Komagataeibacter strains support the agro-waste conversion in bacterial cellulose.					
37115254	9	67	theme	acetic	1524:1529	arg1	bacteria					1536:1543	acetic acid bacteria	1524:1543	acetic acid bacteria	1524:1543	This study proves the suitability to perform sustainable bioprocesses for producing bacterial cellulose, combining the valorisation of agro-wastes with microbial conversions carried out by acetic acid bacteria.					
37115254	8	68	theme	16S	1217:1219	arg1	sequencing					1231:1240	16S rRNA gene sequencing	1217:1240	16S rRNA gene sequencing	1217:1240	The acetic acid bacteria strains used in this study were characterized by 16S rRNA gene sequencing, allowing to assign them to Komagataeibacter xylinus and Komagataeibacter rhaeticus species.					
37115254	10	69	theme	mill	1652:1655	arg1	wastewater					1657:1666	olive mill wastewater	1646:1666	olive mill wastewater	1646:1666	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	5	70	theme	mill	743:746	arg1	wastewater					748:757	olive mill wastewater	737:757	olive mill wastewater	737:757	Bacterial cellulose produced in olive mill wastewater presented a more well-defined network compared to pellicles produced in cheese whey, resulting in a smaller fiber diameter in most cases.					
37115254	2	71	theme	phenolic	280:287	arg1	compounds					289:297	phenolic compounds	280:297	phenolic compounds	280:297	Organic acids and phenolic compounds composition were assayed by high-pressure liquid chromatography.					
37115254	8	72	theme	gene	1226:1229	arg1	sequencing					1231:1240	16S rRNA gene sequencing	1217:1240	16S rRNA gene sequencing	1217:1240	The acetic acid bacteria strains used in this study were characterized by 16S rRNA gene sequencing, allowing to assign them to Komagataeibacter xylinus and Komagataeibacter rhaeticus species.					
37115254	4	73	theme	cellulose/gram	662:675	arg1	0.300 g					641:647	0.300 g	641:647	0.300 g of bacterial cellulose/gram of carbon source consumed	641:701	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	10	74	theme	fundamental	1689:1699	arg1	criteria					1701:1708	fundamental criteria	1689:1708	fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose	1689:1801	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	5	75	theme	most	885:888	arg1	cases					890:894	most cases	885:894	most cases	885:894	Bacterial cellulose produced in olive mill wastewater presented a more well-defined network compared to pellicles produced in cheese whey, resulting in a smaller fiber diameter in most cases.					
37115254	6	76	theme	likely	1005:1010	arg1	bonds					999:1003	different chemical bonds	980:1003	different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components	980:1093	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	4	77	theme	carbon	680:685	arg1	source					687:692	carbon source	680:692	carbon source	680:692	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	12	78	theme	cellulose	1916:1924	arg1	structure					1926:1934	• Bacterial cellulose structure	1904:1934	• Bacterial cellulose structure	1904:1934	• Bacterial cellulose structure is dependent on the culture medium.					
37115254	13	79	from	conversion	2022:2031	arg1	cellulose					2046:2054	bacterial cellulose	2036:2054	bacterial cellulose	2036:2054	• Komagataeibacter strains support the agro-waste conversion in bacterial cellulose.					
37115254	6	80	theme	chemical	990:997	arg1	bonds					999:1003	different chemical bonds	980:1003	different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components	980:1093	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	13	81	theme	•	1972:1972	arg1	strains					1991:1997	• Komagataeibacter strains	1972:1997	• Komagataeibacter strains	1972:1997	• Komagataeibacter strains support the agro-waste conversion in bacterial cellulose.					
37115254	11	82	theme	bacterial	1872:1880	arg1	production					1892:1901	bacterial cellulose production	1872:1901	bacterial cellulose production	1872:1901	KEY POINTS: • Cheese whey and olive mill wastewater can be used for bacterial cellulose production.					
37115254	6	83	theme	cheese	1072:1077	arg1	components					1084:1093	cheese whey components	1072:1093	cheese whey components	1072:1093	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	11	84	theme	Cheese	1818:1823	arg1	whey					1825:1828	• Cheese whey	1816:1828	• Cheese whey	1816:1828	KEY POINTS: • Cheese whey and olive mill wastewater can be used for bacterial cellulose production.					
37115254	11	85	dep	POINTS	1808:1813	arg1	used					1863:1866	used	1863:1866	can be used for bacterial cellulose production	1856:1901	KEY POINTS: • Cheese whey and olive mill wastewater can be used for bacterial cellulose production.					
37115254	1	86	theme	cheese	109:114	arg1	whey					116:119	cheese whey and olive mill wastewater	109:145	whey	116:119	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	1	86	theme	cheese	109:114	arg1	feedstocks					178:187	potential feedstocks	168:187	potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains	168:259	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	1	86	theme	cheese	109:114	arg1	wastewater					136:145	cheese whey and olive mill wastewater	109:145	wastewater	136:145	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	6	87	theme	wastewater	1057:1066	arg1	adsorption					1032:1041	the adsorption	1028:1041	the adsorption of olive mill wastewater and cheese whey components	1028:1093	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	0	88	theme	mill	64:67	arg1	wastewater					69:78	cheese whey and olive mill wastewater	42:78	wastewater	69:78	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.					
37115254	12	89	theme	Bacterial	1906:1914	arg1	structure					1926:1934	• Bacterial cellulose structure	1904:1934	• Bacterial cellulose structure	1904:1934	• Bacterial cellulose structure is dependent on the culture medium.					
37115254	6	90	theme	olive	1046:1050	arg1	wastewater					1057:1066	olive mill wastewater	1046:1066	olive mill wastewater	1046:1066	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	4	91	theme	Cheese	557:562	arg1	feedstock					592:600	the most efficient feedstock	573:600	the most efficient feedstock	573:600	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	4	91	theme	Cheese	557:562	arg1	whey					564:567	Cheese whey	557:567	Cheese whey	557:567	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	11	92	theme	mill	1840:1843	arg1	wastewater					1845:1854	olive mill wastewater	1834:1854	olive mill wastewater	1834:1854	KEY POINTS: • Cheese whey and olive mill wastewater can be used for bacterial cellulose production.					
37115254	11	93	used	used	1863:1866	arg2	wastewater					1845:1854	olive mill wastewater	1834:1854	olive mill wastewater	1834:1854	KEY POINTS: • Cheese whey and olive mill wastewater can be used for bacterial cellulose production.					
37115254	11	93	used	used	1863:1866	arg2	whey					1825:1828	• Cheese whey	1816:1828	• Cheese whey	1816:1828	KEY POINTS: • Cheese whey and olive mill wastewater can be used for bacterial cellulose production.					
37115254	3	94	theme	Fourier-transform	364:380	arg1	spectroscopy					391:402	Fourier-transform infrared spectroscopy	364:402	Fourier-transform infrared spectroscopy	364:402	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	10	95	theme	final	1766:1770	arg1	use					1772:1774	the final use	1762:1774	the final use of the bacterial cellulose	1762:1801	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	12	96	theme	culture	1956:1962	arg1	medium					1964:1969	the culture medium	1952:1969	the culture medium	1952:1969	• Bacterial cellulose structure is dependent on the culture medium.					
37115254	8	97	dep	Komagataeibacter	1299:1314	arg1	rhaeticus					1316:1324	Komagataeibacter rhaeticus	1299:1324	Komagataeibacter rhaeticus	1299:1324	The acetic acid bacteria strains used in this study were characterized by 16S rRNA gene sequencing, allowing to assign them to Komagataeibacter xylinus and Komagataeibacter rhaeticus species.					
37115254	0	98	theme	acid	7:10	arg1	bacteria					12:19	Acetic acid bacteria	0:19	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.	0:92	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.					
37115254	3	99	theme	scanning	405:412	arg1	microscopy					423:432	scanning electron microscopy	405:432	scanning electron microscopy	405:432	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	5	100	theme	smaller	859:865	arg1	diameter					873:880	a smaller fiber diameter	857:880	a smaller fiber diameter in most cases	857:894	Bacterial cellulose produced in olive mill wastewater presented a more well-defined network compared to pellicles produced in cheese whey, resulting in a smaller fiber diameter in most cases.					
37115254	6	101	theme	components	1084:1093	arg1	adsorption					1032:1041	the adsorption	1028:1041	the adsorption of olive mill wastewater and cheese whey components	1028:1093	The analysis of bacterial cellulose chemical structure highlighted the presence of different chemical bonds likely to be caused by the adsorption of olive mill wastewater and cheese whey components.					
37115254	0	102	theme	cheese	42:47	arg1	whey					49:52	cheese whey and olive mill wastewater	42:78	whey	49:52	Acetic acid bacteria in agro-wastes: from cheese whey and olive mill wastewater to cellulose.					
37115254	8	103	theme	acetic	1147:1152	arg1	bacteria					1159:1166	The acetic acid bacteria	1143:1166	The acetic acid bacteria strains used in this study	1143:1193	The acetic acid bacteria strains used in this study were characterized by 16S rRNA gene sequencing, allowing to assign them to Komagataeibacter xylinus and Komagataeibacter rhaeticus species.					
37115254	10	104	theme	morphology	1586:1595	arg1	terms					1570:1574	terms	1570:1574	terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater	1570:1666	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	3	105	from	modifications	482:494	arg1	structure					546:554	bacterial cellulose chemical and morphological structure	499:554	bacterial cellulose chemical and morphological structure	499:554	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	8	106	theme	bacteria	1159:1166	arg1	strains					1168:1174	The acetic acid bacteria strains	1143:1174	The acetic acid bacteria strains used in this study	1143:1193	The acetic acid bacteria strains used in this study were characterized by 16S rRNA gene sequencing, allowing to assign them to Komagataeibacter xylinus and Komagataeibacter rhaeticus species.					
37115254	10	107	theme	yield	1579:1583	arg1	terms					1570:1574	terms	1570:1574	terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater	1570:1666	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	3	108	theme	cellulose	509:517	arg1	structure					546:554	bacterial cellulose chemical and morphological structure	499:554	bacterial cellulose chemical and morphological structure	499:554	Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate modifications in bacterial cellulose chemical and morphological structure.					
37115254	1	109	theme	bacterial	203:211	arg1	strains					253:259	bacterial cellulose by using acetic acid bacteria strains	203:259	bacterial cellulose by using acetic acid bacteria strains	203:259	In this study, cheese whey and olive mill wastewater were investigated as potential feedstocks for producing bacterial cellulose by using acetic acid bacteria strains.					
37115254	10	110	from	bioprocesses	1736:1747	arg1	use					1772:1774	the final use	1762:1774	the final use of the bacterial cellulose	1762:1801	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	10	111	theme	cellulose	1793:1801	arg1	use					1772:1774	the final use	1762:1774	the final use of the bacterial cellulose	1762:1801	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	4	112	theme	bacterial	652:660	arg1	cellulose/gram					662:675	bacterial cellulose/gram	652:675	bacterial cellulose/gram of carbon source consumed	652:701	Cheese whey was the most efficient feedstock in terms of bacterial cellulose yield (0.300 g of bacterial cellulose/gram of carbon source consumed).					
37115254	10	113	theme	diameters	1608:1616	arg1	terms					1570:1574	terms	1570:1574	terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater	1570:1666	The high versatility in terms of yield, morphology, and fiber diameters obtained in cheese whey and olive mill wastewater contribute to set up fundamental criteria for developing customized bioprocesses depending on the final use of the bacterial cellulose.					
37115254	9	114	theme	agro-wastes	1470:1480	arg1	valorisation					1454:1465	the valorisation	1450:1465	the valorisation of agro-wastes	1450:1480	This study proves the suitability to perform sustainable bioprocesses for producing bacterial cellulose, combining the valorisation of agro-wastes with microbial conversions carried out by acetic acid bacteria.					
36935042	9	0	theme	anti-inflammatory	1570:1586	arg1	beta-1					1621:1626	transforming growth factor beta-1	1594:1626	transforming growth factor beta-1 (tfg-β1)	1594:1635	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	0	theme	anti-inflammatory	1570:1586	arg1	gene					1588:1591	anti-inflammatory gene	1570:1591	anti-inflammatory gene	1570:1591	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	6	1	theme	control	1092:1098	arg1	group					1100:1104	the control group	1088:1104	the control group	1088:1104	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	7	2	theme	prebiotic	1120:1128	arg1	doses					1130:1134	prebiotic doses	1120:1134	prebiotic doses	1120:1134	In addition, prebiotic doses significantly induced an up-regulation of heat shock cognate 70kDa protein (hsp70) in the liver compared to the control group.					
36935042	12	3	theme	Vagococcus	2280:2289	arg1	fluvialis					2291:2299	Vagococcus fluvialis	2280:2299	Vagococcus fluvialis	2280:2299	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	1	4	theme	Asian	230:234	arg1	seabass					236:242	juvenile Asian seabass	221:242	juvenile Asian seabass	221:242	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	1	4	theme	Asian	230:234	arg1	calcarifer					251:260	calcarifer	251:260	calcarifer	251:260	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	9	5	theme	growth	1607:1612	arg1	tfg-β1					1629:1634	tfg-β1	1629:1634	tfg-β1	1629:1634	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	5	theme	growth	1607:1612	arg1	beta-1					1621:1626	transforming growth factor beta-1	1594:1626	transforming growth factor beta-1 (tfg-β1)	1594:1635	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	5	theme	growth	1607:1612	arg1	gene					1588:1591	anti-inflammatory gene	1570:1591	anti-inflammatory gene	1570:1591	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	11	6	theme	%	2045:2045	arg1	prebiotic					2064:2072	0.3% Aspergillus-meal prebiotic	2042:2072	0.3% Aspergillus-meal prebiotic	2042:2072	Additionally, the α-diversity of gut microbiota, including genus, Pielou's evenness, Shannon diversity index, and Margalef's species richness were significantly higher in fish fed 0.3% Aspergillus-meal prebiotic than the control group.					
36935042	12	7	theme	garvieae	2383:2390	arg1	subsp					2392:2396	Staphylococcus sciuri and L. garvieae subsp	2354:2396	Staphylococcus sciuri and L. garvieae subsp	2354:2396	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	1	8	theme	growth	158:163	arg1	performance					165:175	growth performance	158:175	growth performance	158:175	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	11	9	theme	diversity	1955:1963	arg1	index					1965:1969	Shannon diversity index	1947:1969	Shannon diversity index	1947:1969	Additionally, the α-diversity of gut microbiota, including genus, Pielou's evenness, Shannon diversity index, and Margalef's species richness were significantly higher in fish fed 0.3% Aspergillus-meal prebiotic than the control group.					
36935042	6	10	theme	prebiotic	933:941	arg1	Supplementation					893:907	Supplementation	893:907	Supplementation of the Aspergillus-meal prebiotic	893:941	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	8	11	theme	Signaling	1263:1271	arg1	pathways					1273:1280	Signaling pathways	1263:1280	Signaling pathways	1263:1280	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	13	12	theme	Aspergillus-meal	2442:2457	arg1	prebiotic					2459:2467	Aspergillus-meal prebiotic	2442:2467	Aspergillus-meal prebiotic	2442:2467	garvieae were present in fish treated with Aspergillus-meal prebiotic.					
36935042	3	13	theme	acceptable	556:565	arg1	quality					573:579	acceptable water quality	556:579	acceptable water quality	556:579	Fish were raised in freshwater with acceptable water quality.					
36935042	8	14	theme	lethal	1421:1426	arg1	expression					1332:1341	significantly higher gene expression	1306:1341	significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic	1306:1508	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	4	15	theme	muscle	677:682	arg1	composition					650:660	composition	650:660	composition	650:660	No significant differences were found in the growth performance and composition of dorsal fish muscle among all groups.					
36935042	4	15	theme	muscle	677:682	arg1	performance					634:644	growth performance	627:644	growth performance	627:644	No significant differences were found in the growth performance and composition of dorsal fish muscle among all groups.					
36935042	5	16	theme	control	879:885	arg1	diet					887:890	the control diet	875:890	the control diet	875:890	Fish fed diets supplemented with 0.3% of Aspergillus-meal prebiotic had a significantly higher survival rate after being challenged with V. alginolyticus than fish fed with the control diet.					
36935042	8	17	theme	SEC13	1433:1437	arg1	protein					1439:1445	SEC13 protein 8	1433:1447	SEC13 protein 8 (mlst-8)	1433:1456	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	8	17	theme	SEC13	1433:1437	arg1	mlst-8					1450:1455	mlst-8	1450:1455	mlst-8	1450:1455	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	14	18	theme	seabass	2562:2568	arg1	performance					2541:2551	the growth performance	2530:2551	the growth performance of Asian seabass	2530:2568	Although dietary Aspergillus-meal prebiotic did not improve the growth performance of Asian seabass, 0.3% of Aspergillus-meal prebiotic is recommended to elevate the immunological status of fish.					
36935042	4	19	located	found	614:618	arg1	composition					650:660	composition	650:660	composition	650:660	No significant differences were found in the growth performance and composition of dorsal fish muscle among all groups.					
36935042	4	19	located	found	614:618	arg2	differences					597:607	No significant differences	582:607	No significant differences	582:607	No significant differences were found in the growth performance and composition of dorsal fish muscle among all groups.					
36935042	4	19	located	found	614:618	arg1	performance					634:644	growth performance	627:644	growth performance	627:644	No significant differences were found in the growth performance and composition of dorsal fish muscle among all groups.					
36935042	5	20	theme	higher	790:795	arg1	rate					806:809	a significantly higher survival rate	774:809	a significantly higher survival rate	774:809	Fish fed diets supplemented with 0.3% of Aspergillus-meal prebiotic had a significantly higher survival rate after being challenged with V. alginolyticus than fish fed with the control diet.					
36935042	13	21	from	present	2413:2419	arg1	fish					2424:2427	fish	2424:2427	fish treated with Aspergillus-meal prebiotic	2424:2467	garvieae were present in fish treated with Aspergillus-meal prebiotic.					
36935042	6	22	theme	respiratory	1002:1012	arg1	burst					1014:1018	higher respiratory burst	995:1018	higher respiratory burst	995:1018	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	6	22	theme	respiratory	1002:1012	arg1	dismutase					1032:1040	superoxide dismutase	1021:1040	superoxide dismutase	1021:1040	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	6	22	theme	respiratory	1002:1012	arg1	activity					1067:1074	phagocytic and lysozyme activity	1043:1074	phagocytic and lysozyme activity	1043:1074	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	9	23	theme	fish	1685:1688	arg1	kidney					1675:1680	the head kidney	1666:1680	the head kidney of fish offered prebiotic diets	1666:1712	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	4	24	theme	dorsal	665:670	arg1	muscle					677:682	dorsal fish muscle	665:682	dorsal fish muscle	665:682	No significant differences were found in the growth performance and composition of dorsal fish muscle among all groups.					
36935042	0	25	theme	health	70:75	arg1	status					77:82	health status	70:82	health status	70:82	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	1	26	theme	prebiotic	311:319	arg1	product					321:327	a prebiotic product	309:327	a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®	309:381	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	13	27	located	present	2413:2419	arg2	garvieae					2399:2406	garvieae	2399:2406	garvieae	2399:2406	garvieae were present in fish treated with Aspergillus-meal prebiotic.					
36935042	13	27	located	present	2413:2419	arg1	fish					2424:2427	fish	2424:2427	fish treated with Aspergillus-meal prebiotic	2424:2467	garvieae were present in fish treated with Aspergillus-meal prebiotic.					
36935042	14	28	theme	growth	2534:2539	arg1	performance					2541:2551	the growth performance	2530:2551	the growth performance of Asian seabass	2530:2568	Although dietary Aspergillus-meal prebiotic did not improve the growth performance of Asian seabass, 0.3% of Aspergillus-meal prebiotic is recommended to elevate the immunological status of fish.					
36935042	2	29	theme	Aspergillus-meal	480:495	arg1	prebiotic					497:505	0.3% Aspergillus-meal prebiotic	475:505	0.3% Aspergillus-meal prebiotic	475:505	Asian seabass were fed three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic for 56 days.					
36935042	2	29	theme	Aspergillus-meal	480:495	arg1	diets					413:417	three diets	407:417	three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic	407:505	Asian seabass were fed three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic for 56 days.					
36935042	0	30	from	Effects	0:6	arg1	performance					57:67	growth performance	50:67	growth performance	50:67	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	0	30	from	Effects	0:6	arg1	status					77:82	health status	70:82	health status	70:82	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	0	30	from	Effects	0:6	arg1	microbiota					92:101	gut microbiota	88:101	gut microbiota	88:101	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	12	31	theme	high	2163:2166	arg1	abundance					2168:2176	a high abundance	2161:2176	a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis,	2161:2300	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	2	32	theme	0.3	475:477	arg1	%					478:478	%	478:478	%	478:478	Asian seabass were fed three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic for 56 days.					
36935042	12	33	theme	beneficial	2181:2190	arg1	fluvialis					2291:2299	Vagococcus fluvialis	2280:2299	Vagococcus fluvialis	2280:2299	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	12	33	theme	beneficial	2181:2190	arg1	faecium					2222:2228	Entercoccus faecium	2210:2228	Entercoccus faecium	2210:2228	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	12	33	theme	beneficial	2181:2190	arg1	bacteria					2192:2199	beneficial bacteria	2181:2199	beneficial bacteria	2181:2199	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	12	33	theme	beneficial	2181:2190	arg1	caseolyticus					2263:2274	Macrococcus caseolyticus	2251:2274	Macrococcus caseolyticus	2251:2274	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	12	33	theme	beneficial	2181:2190	arg1	lactis					2243:2248	Lactococcus lactis	2231:2248	Lactococcus lactis	2231:2248	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	8	34	theme	c-3	1357:1359	arg1	expression					1332:1341	significantly higher gene expression	1306:1341	significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic	1306:1508	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	7	35	theme	70kDa	1197:1201	arg1	protein					1203:1209	heat shock cognate 70kDa protein	1178:1209	heat shock cognate 70kDa protein (hsp70)	1178:1217	In addition, prebiotic doses significantly induced an up-regulation of heat shock cognate 70kDa protein (hsp70) in the liver compared to the control group.					
36935042	7	35	theme	70kDa	1197:1201	arg1	hsp70					1212:1216	hsp70	1212:1216	hsp70	1212:1216	In addition, prebiotic doses significantly induced an up-regulation of heat shock cognate 70kDa protein (hsp70) in the liver compared to the control group.					
36935042	8	36	theme	fish	1474:1477	arg1	liver					1465:1469	the liver	1461:1469	the liver of fish fed 0.3% Aspergillus prebiotic	1461:1508	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	7	37	theme	shock	1183:1187	arg1	protein					1203:1209	heat shock cognate 70kDa protein	1178:1209	heat shock cognate 70kDa protein (hsp70)	1178:1217	In addition, prebiotic doses significantly induced an up-regulation of heat shock cognate 70kDa protein (hsp70) in the liver compared to the control group.					
36935042	7	37	theme	shock	1183:1187	arg1	hsp70					1212:1216	hsp70	1212:1216	hsp70	1212:1216	In addition, prebiotic doses significantly induced an up-regulation of heat shock cognate 70kDa protein (hsp70) in the liver compared to the control group.					
36935042	6	38	theme	lysozyme	1058:1065	arg1	activity					1067:1074	phagocytic and lysozyme activity	1043:1074	phagocytic and lysozyme activity	1043:1074	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	6	38	theme	lysozyme	1058:1065	arg1	burst					1014:1018	higher respiratory burst	995:1018	higher respiratory burst	995:1018	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	8	39	theme	0.3	1483:1485	arg1	%					1486:1486	%	1486:1486	%	1486:1486	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	12	40	theme	principal	2102:2110	arg1	analysis					2122:2129	The principal component analysis	2098:2129	The principal component analysis eigenvector plots	2098:2147	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	0	41	theme	Aspergillus-meal	11:26	arg1	diet					38:41	Aspergillus-meal prebiotic diet	11:41	Aspergillus-meal prebiotic diet	11:41	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	0	42	dep	performance	57:67	arg1	the					46:48	the	46:48	the	46:48	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	6	43	theme	phagocytic	1043:1052	arg1	activity					1067:1074	phagocytic and lysozyme activity	1043:1074	phagocytic and lysozyme activity	1043:1074	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	6	43	theme	phagocytic	1043:1052	arg1	burst					1014:1018	higher respiratory burst	995:1018	higher respiratory burst	995:1018	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	8	44	theme	Aspergillus	1488:1498	arg1	prebiotic					1500:1508	0.3% Aspergillus prebiotic	1483:1508	0.3% Aspergillus prebiotic	1483:1508	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	10	45	theme	h	1797:1797	arg1	expression					1772:1781	significantly higher expression	1751:1781	significantly higher expression of Mx gene 24 h post nervous necrosis virus injection	1751:1835	Fish receiving Aspergillus revealed significantly higher expression of Mx gene 24 h post nervous necrosis virus injection compared to the control.					
36935042	8	46	theme	target	1379:1384	arg1	expression					1332:1341	significantly higher gene expression	1306:1341	significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic	1306:1508	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	0	47	theme	diet	38:41	arg1	Effects					0:6	Effects	0:6	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates	0:125	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	9	48	theme	tumor	1538:1542	arg1	tnf					1561:1563	tnf	1561:1563	tnf	1561:1563	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	48	theme	tumor	1538:1542	arg1	factor					1553:1558	tumor necrosis factor	1538:1558	tumor necrosis factor (tnf)	1538:1564	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	48	theme	tumor	1538:1542	arg1	gene					1532:1535	The pro-inflammatory gene	1511:1535	The pro-inflammatory gene	1511:1535	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	10	49	theme	gene	1789:1792	arg1	h					1797:1797	Mx gene 24 h	1786:1797	Mx gene 24 h post nervous necrosis virus injection	1786:1835	Fish receiving Aspergillus revealed significantly higher expression of Mx gene 24 h post nervous necrosis virus injection compared to the control.					
36935042	8	50	theme	rapamycin	1389:1397	arg1	c3					1362:1363	c3	1362:1363	c3	1362:1363	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	8	50	theme	rapamycin	1389:1397	arg1	c-3					1357:1359	complement c-3	1346:1359	complement c-3 (c3)	1346:1364	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	8	50	theme	rapamycin	1389:1397	arg1	target					1379:1384	mechanistic target	1367:1384	mechanistic target of rapamycin (mtor)	1367:1404	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	5	51	theme	prebiotic	760:768	arg1	%					738:738	0.3%	735:738	0.3% of Aspergillus-meal prebiotic	735:768	Fish fed diets supplemented with 0.3% of Aspergillus-meal prebiotic had a significantly higher survival rate after being challenged with V. alginolyticus than fish fed with the control diet.					
36935042	5	51	theme	prebiotic	760:768	arg1	prebiotic					760:768	Aspergillus-meal prebiotic	743:768	Aspergillus-meal prebiotic	743:768	Fish fed diets supplemented with 0.3% of Aspergillus-meal prebiotic had a significantly higher survival rate after being challenged with V. alginolyticus than fish fed with the control diet.					
36935042	1	52	theme	dietary	283:289	arg1	administration					291:304	dietary administration	283:304	dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®	283:381	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	14	53	theme	dietary	2479:2485	arg1	prebiotic					2504:2512	dietary Aspergillus-meal prebiotic	2479:2512	dietary Aspergillus-meal prebiotic	2479:2512	Although dietary Aspergillus-meal prebiotic did not improve the growth performance of Asian seabass, 0.3% of Aspergillus-meal prebiotic is recommended to elevate the immunological status of fish.					
36935042	10	54	theme	higher	1765:1770	arg1	expression					1772:1781	significantly higher expression	1751:1781	significantly higher expression of Mx gene 24 h post nervous necrosis virus injection	1751:1835	Fish receiving Aspergillus revealed significantly higher expression of Mx gene 24 h post nervous necrosis virus injection compared to the control.					
36935042	10	55	theme	virus	1821:1825	arg1	injection					1827:1835	nervous necrosis virus injection	1804:1835	nervous necrosis virus injection	1804:1835	Fish receiving Aspergillus revealed significantly higher expression of Mx gene 24 h post nervous necrosis virus injection compared to the control.					
36935042	12	56	theme	pathogenic	2325:2334	arg1	bacteria					2336:2343	potentially pathogenic bacteria	2313:2343	potentially pathogenic bacteria	2313:2343	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	12	56	theme	pathogenic	2325:2334	arg1	subsp					2392:2396	Staphylococcus sciuri and L. garvieae subsp	2354:2396	Staphylococcus sciuri and L. garvieae subsp	2354:2396	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	10	57	theme	nervous	1804:1810	arg1	virus					1821:1825	nervous necrosis virus	1804:1825	nervous necrosis virus injection	1804:1835	Fish receiving Aspergillus revealed significantly higher expression of Mx gene 24 h post nervous necrosis virus injection compared to the control.					
36935042	1	58	dep	performance	165:175	arg1	the					154:156	the	154:156	the	154:156	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	8	59	from	expression	1332:1341	arg1	liver					1465:1469	the liver	1461:1469	the liver of fish fed 0.3% Aspergillus prebiotic	1461:1508	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	5	60	theme	Aspergillus-meal	743:758	arg1	prebiotic					760:768	Aspergillus-meal prebiotic	743:768	Aspergillus-meal prebiotic	743:768	Fish fed diets supplemented with 0.3% of Aspergillus-meal prebiotic had a significantly higher survival rate after being challenged with V. alginolyticus than fish fed with the control diet.					
36935042	14	61	theme	immunological	2636:2648	arg1	status					2650:2655	the immunological status	2632:2655	the immunological status of fish	2632:2663	Although dietary Aspergillus-meal prebiotic did not improve the growth performance of Asian seabass, 0.3% of Aspergillus-meal prebiotic is recommended to elevate the immunological status of fish.					
36935042	1	62	theme	juvenile	221:228	arg1	seabass					236:242	juvenile Asian seabass	221:242	juvenile Asian seabass	221:242	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	1	62	theme	juvenile	221:228	arg1	calcarifer					251:260	calcarifer	251:260	calcarifer	251:260	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	11	63	theme	0.3	2042:2044	arg1	%					2045:2045	%	2045:2045	%	2045:2045	Additionally, the α-diversity of gut microbiota, including genus, Pielou's evenness, Shannon diversity index, and Margalef's species richness were significantly higher in fish fed 0.3% Aspergillus-meal prebiotic than the control group.					
36935042	13	64	attach	present	2413:2419	arg2	garvieae					2399:2406	garvieae	2399:2406	garvieae	2399:2406	garvieae were present in fish treated with Aspergillus-meal prebiotic.					
36935042	13	64	attach	present	2413:2419	arg1	fish					2424:2427	fish	2424:2427	fish treated with Aspergillus-meal prebiotic	2424:2467	garvieae were present in fish treated with Aspergillus-meal prebiotic.					
36935042	12	65	theme	analysis	2122:2129	arg1	plots					2143:2147	The principal component analysis eigenvector plots	2098:2147	The principal component analysis eigenvector plots	2098:2147	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	1	66	theme	seabass	236:242	arg1	microbiota					207:216	intestinal microbiota	196:216	intestinal microbiota	196:216	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	1	66	theme	seabass	236:242	arg1	performance					165:175	growth performance	158:175	growth performance	158:175	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	1	66	theme	seabass	236:242	arg1	status					185:190	health status	178:190	health status	178:190	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	9	67	theme	transforming	1594:1605	arg1	tfg-β1					1629:1634	tfg-β1	1629:1634	tfg-β1	1629:1634	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	67	theme	transforming	1594:1605	arg1	beta-1					1621:1626	transforming growth factor beta-1	1594:1626	transforming growth factor beta-1 (tfg-β1)	1594:1635	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	67	theme	transforming	1594:1605	arg1	gene					1588:1591	anti-inflammatory gene	1570:1591	anti-inflammatory gene	1570:1591	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	8	68	theme	gene	1327:1330	arg1	expression					1332:1341	significantly higher gene expression	1306:1341	significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic	1306:1508	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	9	69	theme	prebiotic	1698:1706	arg1	diets					1708:1712	prebiotic diets	1698:1712	prebiotic diets	1698:1712	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	70	theme	factor	1614:1619	arg1	tfg-β1					1629:1634	tfg-β1	1629:1634	tfg-β1	1629:1634	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	70	theme	factor	1614:1619	arg1	beta-1					1621:1626	transforming growth factor beta-1	1594:1626	transforming growth factor beta-1 (tfg-β1)	1594:1635	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	70	theme	factor	1614:1619	arg1	gene					1588:1591	anti-inflammatory gene	1570:1591	anti-inflammatory gene	1570:1591	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	5	71	theme	V.	839:840	arg1	alginolyticus					842:854	V. alginolyticus	839:854	V. alginolyticus than fish fed with the control diet	839:890	Fish fed diets supplemented with 0.3% of Aspergillus-meal prebiotic had a significantly higher survival rate after being challenged with V. alginolyticus than fish fed with the control diet.					
36935042	6	72	theme	immune	966:971	arg1	responses					973:981	immune responses	966:981	immune responses	966:981	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	11	73	theme	Aspergillus-meal	2047:2062	arg1	prebiotic					2064:2072	0.3% Aspergillus-meal prebiotic	2042:2072	0.3% Aspergillus-meal prebiotic	2042:2072	Additionally, the α-diversity of gut microbiota, including genus, Pielou's evenness, Shannon diversity index, and Margalef's species richness were significantly higher in fish fed 0.3% Aspergillus-meal prebiotic than the control group.					
36935042	4	74	theme	growth	627:632	arg1	performance					634:644	growth performance	627:644	growth performance	627:644	No significant differences were found in the growth performance and composition of dorsal fish muscle among all groups.					
36935042	11	75	theme	Shannon	1947:1953	arg1	index					1965:1969	Shannon diversity index	1947:1969	Shannon diversity index	1947:1969	Additionally, the α-diversity of gut microbiota, including genus, Pielou's evenness, Shannon diversity index, and Margalef's species richness were significantly higher in fish fed 0.3% Aspergillus-meal prebiotic than the control group.					
36935042	5	76	theme	survival	797:804	arg1	rate					806:809	a significantly higher survival rate	774:809	a significantly higher survival rate	774:809	Fish fed diets supplemented with 0.3% of Aspergillus-meal prebiotic had a significantly higher survival rate after being challenged with V. alginolyticus than fish fed with the control diet.					
36935042	11	77	theme	species	1987:1993	arg1	richness					1995:2002	Margalef's species richness	1976:2002	Margalef's species richness	1976:2002	Additionally, the α-diversity of gut microbiota, including genus, Pielou's evenness, Shannon diversity index, and Margalef's species richness were significantly higher in fish fed 0.3% Aspergillus-meal prebiotic than the control group.					
36935042	4	78	theme	significant	585:595	arg1	differences					597:607	No significant differences	582:607	No significant differences	582:607	No significant differences were found in the growth performance and composition of dorsal fish muscle among all groups.					
36935042	0	79	theme	Lates	121:125	arg1	performance					57:67	growth performance	50:67	growth performance	50:67	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	0	79	theme	Lates	121:125	arg1	status					77:82	health status	70:82	health status	70:82	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	0	79	theme	Lates	121:125	arg1	microbiota					92:101	gut microbiota	88:101	gut microbiota	88:101	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	11	80	theme	gut	1895:1897	arg1	microbiota					1899:1908	gut microbiota	1895:1908	gut microbiota	1895:1908	Additionally, the α-diversity of gut microbiota, including genus, Pielou's evenness, Shannon diversity index, and Margalef's species richness were significantly higher in fish fed 0.3% Aspergillus-meal prebiotic than the control group.					
36935042	1	81	theme	health	178:183	arg1	status					185:190	health status	178:190	health status	178:190	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	6	82	theme	Aspergillus-meal	916:931	arg1	prebiotic					933:941	the Aspergillus-meal prebiotic	912:941	the Aspergillus-meal prebiotic	912:941	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	12	83	theme	sciuri	2369:2374	arg1	subsp					2392:2396	Staphylococcus sciuri and L. garvieae subsp	2354:2396	Staphylococcus sciuri and L. garvieae subsp	2354:2396	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	7	84	theme	protein	1203:1209	arg1	up-regulation					1161:1173	an up-regulation	1158:1173	an up-regulation of heat shock cognate 70kDa protein (hsp70)	1158:1217	In addition, prebiotic doses significantly induced an up-regulation of heat shock cognate 70kDa protein (hsp70) in the liver compared to the control group.					
36935042	5	85	dep	fed	707:709	arg1	had					770:772	had	770:772	fed diets supplemented with 0.3% of Aspergillus-meal prebiotic had a significantly higher survival rate after being challenged with V. alginolyticus than fish fed with the control diet	707:890	Fish fed diets supplemented with 0.3% of Aspergillus-meal prebiotic had a significantly higher survival rate after being challenged with V. alginolyticus than fish fed with the control diet.					
36935042	4	86	theme	fish	672:675	arg1	muscle					677:682	dorsal fish muscle	665:682	dorsal fish muscle	665:682	No significant differences were found in the growth performance and composition of dorsal fish muscle among all groups.					
36935042	10	87	theme	Mx	1786:1787	arg1	h					1797:1797	Mx gene 24 h	1786:1797	Mx gene 24 h post nervous necrosis virus injection	1786:1835	Fish receiving Aspergillus revealed significantly higher expression of Mx gene 24 h post nervous necrosis virus injection compared to the control.					
36935042	6	88	theme	superoxide	1021:1030	arg1	burst					1014:1018	higher respiratory burst	995:1018	higher respiratory burst	995:1018	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	6	88	theme	superoxide	1021:1030	arg1	dismutase					1032:1040	superoxide dismutase	1021:1040	superoxide dismutase	1021:1040	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	9	89	theme	head	1670:1673	arg1	kidney					1675:1680	the head kidney	1666:1680	the head kidney of fish offered prebiotic diets	1666:1712	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	14	90	theme	fish	2660:2663	arg1	status					2650:2655	the immunological status	2632:2655	the immunological status of fish	2632:2663	Although dietary Aspergillus-meal prebiotic did not improve the growth performance of Asian seabass, 0.3% of Aspergillus-meal prebiotic is recommended to elevate the immunological status of fish.					
36935042	3	91	theme	water	567:571	arg1	quality					573:579	acceptable water quality	556:579	acceptable water quality	556:579	Fish were raised in freshwater with acceptable water quality.					
36935042	14	92	theme	Asian	2556:2560	arg1	seabass					2562:2568	Asian seabass	2556:2568	Asian seabass	2556:2568	Although dietary Aspergillus-meal prebiotic did not improve the growth performance of Asian seabass, 0.3% of Aspergillus-meal prebiotic is recommended to elevate the immunological status of fish.					
36935042	6	93	theme	higher	995:1000	arg1	burst					1014:1018	higher respiratory burst	995:1018	higher respiratory burst	995:1018	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	6	93	theme	higher	995:1000	arg1	dismutase					1032:1040	superoxide dismutase	1021:1040	superoxide dismutase	1021:1040	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	6	93	theme	higher	995:1000	arg1	activity					1067:1074	phagocytic and lysozyme activity	1043:1074	phagocytic and lysozyme activity	1043:1074	Supplementation of the Aspergillus-meal prebiotic significantly improved immune responses by inducing higher respiratory burst, superoxide dismutase, phagocytic and lysozyme activity compared to the control group.					
36935042	8	94	theme	higher	1320:1325	arg1	expression					1332:1341	significantly higher gene expression	1306:1341	significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic	1306:1508	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	0	95	theme	gut	88:90	arg1	microbiota					92:101	gut microbiota	88:101	gut microbiota	88:101	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	1	96	theme	product	321:327	arg1	administration					291:304	dietary administration	283:304	dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®	283:381	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
36935042	13	97	from	fish	2424:2427	arg1	present					2413:2419	present	2413:2419	present	2413:2419	garvieae were present in fish treated with Aspergillus-meal prebiotic.					
36935042	2	98	theme	%	478:478	arg1	prebiotic					497:505	0.3% Aspergillus-meal prebiotic	475:505	0.3% Aspergillus-meal prebiotic	475:505	Asian seabass were fed three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic for 56 days.					
36935042	2	98	theme	%	478:478	arg1	diets					413:417	three diets	407:417	three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic	407:505	Asian seabass were fed three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic for 56 days.					
36935042	12	99	theme	bacteria	2192:2199	arg1	abundance					2168:2176	a high abundance	2161:2176	a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis,	2161:2300	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	7	100	theme	cognate	1189:1195	arg1	protein					1203:1209	heat shock cognate 70kDa protein	1178:1209	heat shock cognate 70kDa protein (hsp70)	1178:1217	In addition, prebiotic doses significantly induced an up-regulation of heat shock cognate 70kDa protein (hsp70) in the liver compared to the control group.					
36935042	7	100	theme	cognate	1189:1195	arg1	hsp70					1212:1216	hsp70	1212:1216	hsp70	1212:1216	In addition, prebiotic doses significantly induced an up-regulation of heat shock cognate 70kDa protein (hsp70) in the liver compared to the control group.					
36935042	2	101	dep	diets	413:417	arg1	control					420:426	control	420:426	control (without Aspergillus-meal prebiotic)	420:463	Asian seabass were fed three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic for 56 days.					
36935042	2	101	dep	diets	413:417	arg1	prebiotic					497:505	0.3% Aspergillus-meal prebiotic	475:505	0.3% Aspergillus-meal prebiotic	475:505	Asian seabass were fed three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic for 56 days.					
36935042	2	101	dep	diets	413:417	arg1	%					469:469	0.2%	466:469	0.2%	466:469	Asian seabass were fed three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic for 56 days.					
36935042	2	101	dep	diets	413:417	arg1	diets					413:417	three diets	407:417	three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic	407:505	Asian seabass were fed three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic for 56 days.					
36935042	8	102	theme	complement	1346:1355	arg1	c3					1362:1363	c3	1362:1363	c3	1362:1363	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	8	102	theme	complement	1346:1355	arg1	c-3					1357:1359	complement c-3	1346:1359	complement c-3 (c3)	1346:1364	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	7	103	theme	heat	1178:1181	arg1	protein					1203:1209	heat shock cognate 70kDa protein	1178:1209	heat shock cognate 70kDa protein (hsp70)	1178:1217	In addition, prebiotic doses significantly induced an up-regulation of heat shock cognate 70kDa protein (hsp70) in the liver compared to the control group.					
36935042	7	103	theme	heat	1178:1181	arg1	hsp70					1212:1216	hsp70	1212:1216	hsp70	1212:1216	In addition, prebiotic doses significantly induced an up-regulation of heat shock cognate 70kDa protein (hsp70) in the liver compared to the control group.					
36935042	14	104	theme	prebiotic	2596:2604	arg1	%					2574:2574	0.3%	2571:2574	0.3% of Aspergillus-meal prebiotic	2571:2604	Although dietary Aspergillus-meal prebiotic did not improve the growth performance of Asian seabass, 0.3% of Aspergillus-meal prebiotic is recommended to elevate the immunological status of fish.					
36935042	14	104	theme	prebiotic	2596:2604	arg1	prebiotic					2596:2604	Aspergillus-meal prebiotic	2579:2604	Aspergillus-meal prebiotic	2579:2604	Although dietary Aspergillus-meal prebiotic did not improve the growth performance of Asian seabass, 0.3% of Aspergillus-meal prebiotic is recommended to elevate the immunological status of fish.					
36935042	8	105	theme	%	1486:1486	arg1	prebiotic					1500:1508	0.3% Aspergillus prebiotic	1483:1508	0.3% Aspergillus prebiotic	1483:1508	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	12	106	theme	eigenvector	2131:2141	arg1	plots					2143:2147	The principal component analysis eigenvector plots	2098:2147	The principal component analysis eigenvector plots	2098:2147	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	0	107	theme	prebiotic	28:36	arg1	diet					38:41	Aspergillus-meal prebiotic diet	11:41	Aspergillus-meal prebiotic diet	11:41	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	9	108	theme	pro-inflammatory	1515:1530	arg1	factor					1553:1558	tumor necrosis factor	1538:1558	tumor necrosis factor (tnf)	1538:1564	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	108	theme	pro-inflammatory	1515:1530	arg1	higher					1656:1661	higher	1656:1661	higher	1656:1661	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	108	theme	pro-inflammatory	1515:1530	arg1	gene					1532:1535	The pro-inflammatory gene	1511:1535	The pro-inflammatory gene	1511:1535	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	11	109	theme	control	2083:2089	arg1	group					2091:2095	the control group	2079:2095	the control group	2079:2095	Additionally, the α-diversity of gut microbiota, including genus, Pielou's evenness, Shannon diversity index, and Margalef's species richness were significantly higher in fish fed 0.3% Aspergillus-meal prebiotic than the control group.					
36935042	2	110	theme	Aspergillus-meal	437:452	arg1	prebiotic					454:462	Aspergillus-meal prebiotic	437:462	Aspergillus-meal prebiotic	437:462	Asian seabass were fed three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic for 56 days.					
36935042	8	111	theme	mechanistic	1367:1377	arg1	target					1379:1384	mechanistic target	1367:1384	mechanistic target of rapamycin (mtor)	1367:1404	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	2	112	theme	Asian	384:388	arg1	seabass					390:396	Asian seabass	384:396	Asian seabass	384:396	Asian seabass were fed three diets; control (without Aspergillus-meal prebiotic), 0.2% and 0.3% Aspergillus-meal prebiotic for 56 days.					
36935042	0	113	theme	growth	50:55	arg1	performance					57:67	growth performance	50:67	growth performance	50:67	Effects of Aspergillus-meal prebiotic diet on the growth performance, health status and gut microbiota of Asian seabass, Lates calcarifer.					
36935042	14	114	theme	Aspergillus-meal	2579:2594	arg1	prebiotic					2596:2604	Aspergillus-meal prebiotic	2579:2604	Aspergillus-meal prebiotic	2579:2604	Although dietary Aspergillus-meal prebiotic did not improve the growth performance of Asian seabass, 0.3% of Aspergillus-meal prebiotic is recommended to elevate the immunological status of fish.					
36935042	14	115	theme	Aspergillus-meal	2487:2502	arg1	prebiotic					2504:2512	dietary Aspergillus-meal prebiotic	2479:2512	dietary Aspergillus-meal prebiotic	2479:2512	Although dietary Aspergillus-meal prebiotic did not improve the growth performance of Asian seabass, 0.3% of Aspergillus-meal prebiotic is recommended to elevate the immunological status of fish.					
36935042	9	116	theme	necrosis	1544:1551	arg1	tnf					1561:1563	tnf	1561:1563	tnf	1561:1563	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	116	theme	necrosis	1544:1551	arg1	factor					1553:1558	tumor necrosis factor	1538:1558	tumor necrosis factor (tnf)	1538:1564	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	9	116	theme	necrosis	1544:1551	arg1	gene					1532:1535	The pro-inflammatory gene	1511:1535	The pro-inflammatory gene	1511:1535	The pro-inflammatory gene, tumor necrosis factor (tnf) and anti-inflammatory gene, transforming growth factor beta-1 (tfg-β1) were significantly higher in the head kidney of fish offered prebiotic diets.					
36935042	12	117	theme	component	2112:2120	arg1	analysis					2122:2129	The principal component analysis	2098:2129	The principal component analysis eigenvector plots	2098:2147	The principal component analysis eigenvector plots showed that a high abundance of beneficial bacteria, such as Entercoccus faecium, Lactococcus lactis, Macrococcus caseolyticus and Vagococcus fluvialis, along with potentially pathogenic bacteria, such as Staphylococcus sciuri and L. garvieae subsp.					
36935042	8	118	with	lethal	1421:1426	arg1	protein					1439:1445	SEC13 protein 8	1433:1447	SEC13 protein 8 (mlst-8)	1433:1456	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	8	118	with	lethal	1421:1426	arg1	mlst-8					1450:1455	mlst-8	1450:1455	mlst-8	1450:1455	Signaling pathways were also affected with significantly higher gene expression of complement c-3 (c3), mechanistic target of rapamycin (mtor), and mammalian lethal with SEC13 protein 8 (mlst-8) in the liver of fish fed 0.3% Aspergillus prebiotic.					
36935042	11	119	theme	microbiota	1899:1908	arg1	α-diversity					1880:1890	the α-diversity	1876:1890	the α-diversity	1876:1890	Additionally, the α-diversity of gut microbiota, including genus, Pielou's evenness, Shannon diversity index, and Margalef's species richness were significantly higher in fish fed 0.3% Aspergillus-meal prebiotic than the control group.					
36935042	11	119	theme	microbiota	1899:1908	arg1	higher					2023:2028	higher	2023:2028	higher	2023:2028	Additionally, the α-diversity of gut microbiota, including genus, Pielou's evenness, Shannon diversity index, and Margalef's species richness were significantly higher in fish fed 0.3% Aspergillus-meal prebiotic than the control group.					
36935042	7	120	theme	control	1248:1254	arg1	group					1256:1260	the control group	1244:1260	the control group	1244:1260	In addition, prebiotic doses significantly induced an up-regulation of heat shock cognate 70kDa protein (hsp70) in the liver compared to the control group.					
36935042	10	121	theme	necrosis	1812:1819	arg1	virus					1821:1825	nervous necrosis virus	1804:1825	nervous necrosis virus injection	1804:1835	Fish receiving Aspergillus revealed significantly higher expression of Mx gene 24 h post nervous necrosis virus injection compared to the control.					
36935042	1	122	theme	intestinal	196:205	arg1	microbiota					207:216	intestinal microbiota	196:216	intestinal microbiota	196:216	In this study, the growth performance, health status and intestinal microbiota of juvenile Asian seabass, Lates calcarifer, were assessed after dietary administration of a prebiotic product obtained from fermented Aspergillus orizae, Fermacto®.					
35718096	4	0	theme	lipid	599:603	arg1	esterification					605:618	lipid esterification	599:618	lipid esterification	599:618	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	14	1	theme	novel	2278:2282	arg1	acid					2321:2324	myristoleic acid	2309:2324	myristoleic acid	2309:2324	Further, these data point to intestinal SCD1 as a modulator of gut-liver crosstalk, potentially through the production of novel signaling lipids such as myristoleic acid.					
35718096	14	1	theme	novel	2278:2282	arg1	lipids					2294:2299	novel signaling lipids	2278:2299	novel signaling lipids such as myristoleic acid	2278:2324	Further, these data point to intestinal SCD1 as a modulator of gut-liver crosstalk, potentially through the production of novel signaling lipids such as myristoleic acid.					
35718096	11	2	theme	lipogenic	1799:1807	arg1	genes					1809:1813	de novo lipogenic genes	1791:1813	de novo lipogenic genes	1791:1813	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	15	3	theme	hepatic	2448:2454	arg1	steatosis					2456:2464	hepatic steatosis	2448:2464	hepatic steatosis	2448:2464	These data have important implications to understanding how intestinal SCD1 may modulate risk for post-prandial lipemia, hepatic steatosis, and related pathologies.					
35718096	1	4	theme	saturated	260:268	arg1	acids					276:280	saturated fatty acids	260:280	saturated fatty acids	260:280	Stearoyl-CoA desaturase-1 is an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts.					
35718096	11	5	theme	mice	1847:1850	arg1	intestine					1825:1833	distal intestine	1818:1833	distal intestine of chow-fed mice	1818:1850	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	10	6	theme	genes	1695:1699	arg1	expression					1633:1642	hepatic expression	1625:1642	hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes	1625:1699	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	10	7	from	acid	1580:1583	arg1	lipids					1596:1601	hepatic lipids	1588:1601	hepatic lipids of chow fed iKO mice	1588:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	10	8	with	Consistent	1504:1513	arg1	reduction					1524:1532	the reduction	1520:1532	the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice	1520:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	11	9	theme	distal	1818:1823	arg1	intestine					1825:1833	distal intestine	1818:1833	distal intestine of chow-fed mice	1818:1850	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	4	10	theme	SCD1	681:684	arg1	regulation					656:665	the regulation	652:665	the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues	652:754	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	10	11	theme	acid	1680:1683	arg1	oxidation					1685:1693	related fatty acid oxidation	1666:1693	related fatty acid oxidation	1666:1693	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	4	12	from	SCD1	681:684	arg1	composition					709:719	lipid composition	703:719	lipid composition	703:719	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	4	12	from	SCD1	681:684	arg1	intestine					728:736	intestine	728:736	intestine	728:736	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	4	12	from	SCD1	681:684	arg1	tissues					748:754	other tissues	742:754	other tissues	742:754	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	5	13	theme	distal	859:864	arg1	intestine					872:880	the distal small intestine	855:880	the distal small intestine	855:880	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	10	14	theme	acid	1563:1566	arg1	acid					1580:1583	the monounsaturated fatty acid myristoleic acid	1537:1583	the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice	1537:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	10	15	theme	chow-fed	1717:1724	arg1	mice					1730:1733	chow-fed iKO mice	1717:1733	chow-fed iKO mice	1717:1733	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	10	16	theme	monounsaturated	1541:1555	arg1	acid					1580:1583	the monounsaturated fatty acid myristoleic acid	1537:1583	the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice	1537:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	0	17	theme	gut-liver	122:130	arg1	crosstalk					132:140	gut-liver crosstalk	122:140	gut-liver crosstalk	122:140	SCD1 is nutritionally and spatially regulated in the intestine and influences systemic postprandial lipid homeostasis and gut-liver crosstalk.					
35718096	1	18	theme	resident	211:218	arg1	protein					220:226	an endoplasmic reticulum (ER)-membrane resident protein	172:226	an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts	172:337	Stearoyl-CoA desaturase-1 is an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts.					
35718096	8	19	theme	hepatic	1249:1255	arg1	accumulation					1257:1268	hepatic accumulation	1249:1268	hepatic accumulation of diacylglycerols	1249:1287	Additionally, hepatic accumulation of diacylglycerols is significantly reduced in iKO mice.					
35718096	10	20	theme	hepatic	1588:1594	arg1	lipids					1596:1601	hepatic lipids	1588:1601	hepatic lipids of chow fed iKO mice	1588:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	11	21	theme	intestinal	1753:1762	arg1	SCD1					1764:1767	intestinal SCD1	1753:1767	intestinal SCD1	1753:1767	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	13	22	theme	lipid	2059:2063	arg1	content					2065:2071	lipid content	2059:2071	lipid content	2059:2071	They also demonstrate that intestinal SCD1 modulates lipid content and composition of not only intestinal tissues, but also that of plasma and liver.					
35718096	13	23	theme	intestinal	2033:2042	arg1	SCD1					2044:2047	intestinal SCD1	2033:2047	intestinal SCD1	2033:2047	They also demonstrate that intestinal SCD1 modulates lipid content and composition of not only intestinal tissues, but also that of plasma and liver.					
35718096	5	24	from	enriched	843:850	arg1	colon					893:897	the colon	889:897	the colon of chow-fed mice	889:914	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	5	24	from	enriched	843:850	arg1	intestine					872:880	the distal small intestine	855:880	the distal small intestine	855:880	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	12	25	theme	SCD1	1983:1986	arg1	expression					1969:1978	expression	1969:1978	expression of SCD1 in the intestine	1969:2003	Taken together, these studies reveal a novel pattern of expression of SCD1 in the intestine.					
35718096	10	26	theme	acid	1580:1583	arg1	reduction					1524:1532	the reduction	1520:1532	the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice	1520:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	5	27	theme	chow-fed	902:909	arg1	mice					911:914	chow-fed mice	902:914	chow-fed mice	902:914	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	12	28	from	expression	1969:1978	arg1	intestine					1995:2003	the intestine	1991:2003	the intestine	1991:2003	Taken together, these studies reveal a novel pattern of expression of SCD1 in the intestine.					
35718096	1	29	theme	Stearoyl-CoA	143:154	arg1	desaturase-1					156:167	Stearoyl-CoA desaturase-1	143:167	Stearoyl-CoA desaturase-1	143:167	Stearoyl-CoA desaturase-1 is an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts.					
35718096	6	30	theme	mouse	1002:1006	arg1	model					1008:1012	a mouse model	1000:1012	a mouse model lacking SCD1	1000:1025	We generated a mouse model lacking SCD1 specifically in the intestine (iKO mice).					
35718096	11	31	theme	lipogenic	1884:1892	arg1	diet					1907:1910	a lipogenic high-sucrose diet	1882:1910	a lipogenic high-sucrose diet	1882:1910	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	12	32	from	pattern	1958:1964	arg1	intestine					1995:2003	the intestine	1991:2003	the intestine	1991:2003	Taken together, these studies reveal a novel pattern of expression of SCD1 in the intestine.					
35718096	8	33	theme	iKO	1317:1319	arg1	mice					1321:1324	iKO mice	1317:1324	iKO mice	1317:1324	Additionally, hepatic accumulation of diacylglycerols is significantly reduced in iKO mice.					
35718096	1	34	theme	endoplasmic	175:185	arg1	ER					198:199	ER	198:199	ER	198:199	Stearoyl-CoA desaturase-1 is an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts.					
35718096	1	34	theme	endoplasmic	175:185	arg1	reticulum					187:195	an endoplasmic reticulum	172:195	an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts	172:337	Stearoyl-CoA desaturase-1 is an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts.					
35718096	14	35	dep	crosstalk	2229:2237	arg1	potentially					2240:2250	potentially	2240:2250	potentially	2240:2250	Further, these data point to intestinal SCD1 as a modulator of gut-liver crosstalk, potentially through the production of novel signaling lipids such as myristoleic acid.					
35718096	0	36	theme	postprandial	87:98	arg1	homeostasis					106:116	systemic postprandial lipid homeostasis	78:116	systemic postprandial lipid homeostasis	78:116	SCD1 is nutritionally and spatially regulated in the intestine and influences systemic postprandial lipid homeostasis and gut-liver crosstalk.					
35718096	3	37	theme	SCD1	487:490	arg1	lack					458:461	lack	458:461	lack of hepatic or cutaneous SCD1	458:490	Specifically, lack of hepatic or cutaneous SCD1 results in significant reductions in tissue esterified lipids.					
35718096	15	38	dep	understanding	2369:2381	arg1	modulate					2407:2414	modulate	2407:2414	may modulate risk for post-prandial lipemia, hepatic steatosis, and related pathologies	2403:2489	These data have important implications to understanding how intestinal SCD1 may modulate risk for post-prandial lipemia, hepatic steatosis, and related pathologies.					
35718096	10	39	theme	iKO	1615:1617	arg1	mice					1619:1622	chow fed iKO mice	1606:1622	chow fed iKO mice	1606:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	7	40	theme	plasma	1151:1156	arg1	triacylglycerols					1158:1173	plasma triacylglycerols	1151:1173	plasma triacylglycerols	1151:1173	These mice have significant reductions not only in intestinal lipids, but also in plasma triacylglycerols, diacylglycerols, cholesterol esters, and free cholesterol.					
35718096	11	41	dep	de	1791:1792	arg1	novo					1794:1797	novo	1794:1797	novo	1794:1797	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	13	42	dep	tissues	2112:2118	arg1	only					2096:2099	only	2096:2099	only	2096:2099	They also demonstrate that intestinal SCD1 modulates lipid content and composition of not only intestinal tissues, but also that of plasma and liver.					
35718096	11	43	from	expression	1777:1786	arg1	livers					1863:1868	the livers	1859:1868	the livers of mice fed a lipogenic high-sucrose diet	1859:1910	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	11	43	from	expression	1777:1786	arg1	intestine					1825:1833	distal intestine	1818:1833	distal intestine of chow-fed mice	1818:1850	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	10	44	theme	chow	1606:1609	arg1	mice					1619:1622	chow fed iKO mice	1606:1622	chow fed iKO mice	1606:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	5	45	theme	lipogenic	816:824	arg1	enzymes					826:832	other lipogenic enzymes	810:832	other lipogenic enzymes	810:832	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	4	46	from	tissues	748:754	arg1	regulation					656:665	the regulation	652:665	the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues	652:754	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	4	47	dep	intestine	728:736	arg1	the					724:726	the	724:726	the	724:726	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	3	48	theme	cutaneous	477:485	arg1	SCD1					487:490	hepatic or cutaneous SCD1	466:490	hepatic or cutaneous SCD1	466:490	Specifically, lack of hepatic or cutaneous SCD1 results in significant reductions in tissue esterified lipids.					
35718096	9	49	theme	Comprehensive	1327:1339	arg1	profiling					1360:1368	Comprehensive targeted lipidomic profiling	1327:1368	Comprehensive targeted lipidomic profiling	1327:1368	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	10	50	theme	fatty	1674:1678	arg1	oxidation					1685:1693	related fatty acid oxidation	1666:1693	related fatty acid oxidation	1666:1693	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	4	51	theme	important	581:589	arg1	esterification					605:618	lipid esterification	599:618	lipid esterification	599:618	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	4	51	theme	important	581:589	arg1	intestine					565:573	the intestine	561:573	the intestine	561:573	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	4	51	theme	important	581:589	arg1	site					591:594	an important site	578:594	an important site of lipid esterification	578:618	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	3	52	theme	hepatic	466:472	arg1	SCD1					487:490	hepatic or cutaneous SCD1	466:490	hepatic or cutaneous SCD1	466:490	Specifically, lack of hepatic or cutaneous SCD1 results in significant reductions in tissue esterified lipids.					
35718096	2	53	theme	systemic	427:434	arg1	health					436:441	systemic health	427:441	systemic health	427:441	Previous studies have demonstrated an important role for SCD1 in modulating tissue and systemic health.					
35718096	7	54	theme	intestinal	1120:1129	arg1	lipids					1131:1136	intestinal lipids	1120:1136	intestinal lipids	1120:1136	These mice have significant reductions not only in intestinal lipids, but also in plasma triacylglycerols, diacylglycerols, cholesterol esters, and free cholesterol.					
35718096	9	55	theme	lipidomic	1350:1358	arg1	profiling					1360:1368	Comprehensive targeted lipidomic profiling	1327:1368	Comprehensive targeted lipidomic profiling	1327:1368	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	13	56	theme	intestinal	2101:2110	arg1	tissues					2112:2118	not only intestinal tissues	2092:2118	not only intestinal tissues	2092:2118	They also demonstrate that intestinal SCD1 modulates lipid content and composition of not only intestinal tissues, but also that of plasma and liver.					
35718096	10	57	theme	Sirt1	1655:1659	arg1	genes					1695:1699	Pgc-1α, Sirt1, and related fatty acid oxidation genes	1647:1699	Pgc-1α, Sirt1, and related fatty acid oxidation genes	1647:1699	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	10	58	from	reduction	1524:1532	arg1	lipids					1596:1601	hepatic lipids	1588:1601	hepatic lipids of chow fed iKO mice	1588:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	14	59	theme	myristoleic	2309:2319	arg1	acid					2321:2324	myristoleic acid	2309:2324	myristoleic acid	2309:2324	Further, these data point to intestinal SCD1 as a modulator of gut-liver crosstalk, potentially through the production of novel signaling lipids such as myristoleic acid.					
35718096	10	60	theme	Pgc-1α	1647:1652	arg1	genes					1695:1699	Pgc-1α, Sirt1, and related fatty acid oxidation genes	1647:1699	Pgc-1α, Sirt1, and related fatty acid oxidation genes	1647:1699	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	4	61	from	intestine	728:736	arg1	regulation					656:665	the regulation	652:665	the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues	652:754	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	9	62	theme	consistent	1381:1390	arg1	reduction					1392:1400	a consistent reduction	1379:1400	a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice	1379:1501	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	9	63	from	reduction	1392:1400	arg1	liver					1473:1477	liver	1473:1477	liver	1473:1477	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	9	63	from	reduction	1392:1400	arg1	plasma					1484:1489	plasma	1484:1489	plasma	1484:1489	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	9	63	from	reduction	1392:1400	arg1	intestine					1462:1470	intestine	1462:1470	intestine	1462:1470	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	9	63	from	reduction	1392:1400	arg1	ratios					1452:1457	the myristoleic (14:1) to myristic (14:0) acid ratios	1405:1457	the myristoleic (14:1) to myristic (14:0) acid ratios	1405:1457	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	5	64	theme	diet	981:984	arg1	refeeding					953:961	acute refeeding	947:961	acute refeeding of a high-sucrose diet	947:984	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	12	65	theme	novel	1952:1956	arg1	pattern					1958:1964	a novel pattern	1950:1964	a novel pattern of expression of SCD1 in the intestine	1950:2003	Taken together, these studies reveal a novel pattern of expression of SCD1 in the intestine.					
35718096	15	66	contain	have	2338:2341	arg1	data					2333:2336	These data	2327:2336	These data	2327:2336	These data have important implications to understanding how intestinal SCD1 may modulate risk for post-prandial lipemia, hepatic steatosis, and related pathologies.					
35718096	15	66	contain	have	2338:2341	arg2	implications					2353:2364	important implications	2343:2364	important implications	2343:2364	These data have important implications to understanding how intestinal SCD1 may modulate risk for post-prandial lipemia, hepatic steatosis, and related pathologies.					
35718096	3	67	theme	esterified	536:545	arg1	lipids					547:552	tissue esterified lipids	529:552	tissue esterified lipids	529:552	Specifically, lack of hepatic or cutaneous SCD1 results in significant reductions in tissue esterified lipids.					
35718096	1	68	theme	double	243:248	arg1	bond					250:253	a double bond	241:253	a double bond into saturated fatty acids	241:280	Stearoyl-CoA desaturase-1 is an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts.					
35718096	13	69	theme	tissues	2112:2118	arg1	content					2065:2071	lipid content	2059:2071	lipid content	2059:2071	They also demonstrate that intestinal SCD1 modulates lipid content and composition of not only intestinal tissues, but also that of plasma and liver.					
35718096	13	69	theme	tissues	2112:2118	arg1	composition					2077:2087	composition	2077:2087	composition	2077:2087	They also demonstrate that intestinal SCD1 modulates lipid content and composition of not only intestinal tissues, but also that of plasma and liver.					
35718096	7	70	theme	significant	1085:1095	arg1	reductions					1097:1106	significant reductions	1085:1106	significant reductions	1085:1106	These mice have significant reductions not only in intestinal lipids, but also in plasma triacylglycerols, diacylglycerols, cholesterol esters, and free cholesterol.					
35718096	14	71	theme	signaling	2284:2292	arg1	acid					2321:2324	myristoleic acid	2309:2324	myristoleic acid	2309:2324	Further, these data point to intestinal SCD1 as a modulator of gut-liver crosstalk, potentially through the production of novel signaling lipids such as myristoleic acid.					
35718096	14	71	theme	signaling	2284:2292	arg1	lipids					2294:2299	novel signaling lipids	2278:2299	novel signaling lipids such as myristoleic acid	2278:2324	Further, these data point to intestinal SCD1 as a modulator of gut-liver crosstalk, potentially through the production of novel signaling lipids such as myristoleic acid.					
35718096	2	72	theme	important	378:386	arg1	role					388:391	an important role	375:391	an important role for SCD1 in modulating tissue and systemic health	375:441	Previous studies have demonstrated an important role for SCD1 in modulating tissue and systemic health.					
35718096	9	73	theme	myristoleic	1409:1419	arg1	ratios					1452:1457	the myristoleic (14:1) to myristic (14:0) acid ratios	1405:1457	the myristoleic (14:1) to myristic (14:0) acid ratios	1405:1457	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	4	74	from	impact	693:698	arg1	composition					709:719	lipid composition	703:719	lipid composition	703:719	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	4	74	from	impact	693:698	arg1	intestine					728:736	intestine	728:736	intestine	728:736	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	4	74	from	impact	693:698	arg1	tissues					748:754	other tissues	742:754	other tissues	742:754	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	1	75	theme	fatty	270:274	arg1	acids					276:280	saturated fatty acids	260:280	saturated fatty acids	260:280	Stearoyl-CoA desaturase-1 is an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts.					
35718096	12	76	theme	expression	1969:1978	arg1	pattern					1958:1964	a novel pattern	1950:1964	a novel pattern of expression of SCD1 in the intestine	1950:2003	Taken together, these studies reveal a novel pattern of expression of SCD1 in the intestine.					
35718096	4	77	theme	esterification	605:618	arg1	esterification					605:618	lipid esterification	599:618	lipid esterification	599:618	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	4	77	theme	esterification	605:618	arg1	intestine					565:573	the intestine	561:573	the intestine	561:573	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	4	77	theme	esterification	605:618	arg1	assimilation					624:635	assimilation	624:635	assimilation into the body	624:649	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	4	77	theme	esterification	605:618	arg1	site					591:594	an important site	578:594	an important site of lipid esterification	578:618	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	3	78	theme	significant	503:513	arg1	reductions					515:524	significant reductions	503:524	significant reductions in tissue esterified lipids	503:552	Specifically, lack of hepatic or cutaneous SCD1 results in significant reductions in tissue esterified lipids.					
35718096	10	79	theme	oxidation	1685:1693	arg1	genes					1695:1699	Pgc-1α, Sirt1, and related fatty acid oxidation genes	1647:1699	Pgc-1α, Sirt1, and related fatty acid oxidation genes	1647:1699	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	1	80	theme	reticulum	187:195	arg1	protein					220:226	an endoplasmic reticulum (ER)-membrane resident protein	172:226	an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts	172:337	Stearoyl-CoA desaturase-1 is an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts.					
35718096	10	81	from	lipids	1596:1601	arg1	reduction					1524:1532	the reduction	1520:1532	the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice	1520:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	4	82	theme	intestinal	670:679	arg1	SCD1					681:684	intestinal SCD1	670:684	intestinal SCD1	670:684	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	10	83	theme	myristoleic	1568:1578	arg1	acid					1580:1583	the monounsaturated fatty acid myristoleic acid	1537:1583	the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice	1537:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	10	84	theme	iKO	1726:1728	arg1	mice					1730:1733	chow-fed iKO mice	1717:1733	chow-fed iKO mice	1717:1733	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	5	85	theme	small	866:870	arg1	intestine					872:880	the distal small intestine	855:880	the distal small intestine	855:880	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	10	86	theme	fatty	1557:1561	arg1	acid					1580:1583	the monounsaturated fatty acid myristoleic acid	1537:1583	the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice	1537:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	3	87	from	reductions	515:524	arg1	lipids					547:552	tissue esterified lipids	529:552	tissue esterified lipids	529:552	Specifically, lack of hepatic or cutaneous SCD1 results in significant reductions in tissue esterified lipids.					
35718096	4	88	from	regulation	656:665	arg1	composition					709:719	lipid composition	703:719	lipid composition	703:719	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	4	88	from	regulation	656:665	arg1	intestine					728:736	intestine	728:736	intestine	728:736	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	4	88	from	regulation	656:665	arg1	tissues					748:754	other tissues	742:754	other tissues	742:754	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	1	89	theme	-membrane	201:209	arg1	protein					220:226	an endoplasmic reticulum (ER)-membrane resident protein	172:226	an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts	172:337	Stearoyl-CoA desaturase-1 is an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts.					
35718096	9	90	theme	iKO	1494:1496	arg1	mice					1498:1501	iKO mice	1494:1501	iKO mice	1494:1501	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	11	91	theme	mice	1873:1876	arg1	livers					1863:1868	the livers	1859:1868	the livers of mice fed a lipogenic high-sucrose diet	1859:1910	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	11	92	theme	SCD1	1764:1767	arg1	lack					1745:1748	lack	1745:1748	lack of intestinal SCD1	1745:1767	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	15	93	theme	intestinal	2387:2396	arg1	SCD1					2398:2401	intestinal SCD1	2387:2401	intestinal SCD1	2387:2401	These data have important implications to understanding how intestinal SCD1 may modulate risk for post-prandial lipemia, hepatic steatosis, and related pathologies.					
35718096	14	94	theme	gut-liver	2219:2227	arg1	crosstalk					2229:2237	gut-liver crosstalk	2219:2237	gut-liver crosstalk	2219:2237	Further, these data point to intestinal SCD1 as a modulator of gut-liver crosstalk, potentially through the production of novel signaling lipids such as myristoleic acid.					
35718096	14	95	theme	crosstalk	2229:2237	arg1	modulator					2206:2214	a modulator	2204:2214	a modulator of gut-liver crosstalk, potentially through the production of novel signaling lipids such as myristoleic acid	2204:2324	Further, these data point to intestinal SCD1 as a modulator of gut-liver crosstalk, potentially through the production of novel signaling lipids such as myristoleic acid.					
35718096	11	96	theme	high-sucrose	1894:1905	arg1	diet					1907:1910	a lipogenic high-sucrose diet	1882:1910	a lipogenic high-sucrose diet	1882:1910	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	5	97	theme	mice	911:914	arg1	colon					893:897	the colon	889:897	the colon of chow-fed mice	889:914	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	9	98	dep	myristoleic	1409:1419	arg1	to					1428:1429	to	1428:1429	to	1428:1429	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	9	98	dep	myristoleic	1409:1419	arg1	14:0					1441:1444	14:0	1441:1444	14:0	1441:1444	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	9	98	dep	myristoleic	1409:1419	arg1	14:1					1422:1425	14:1	1422:1425	14:1	1422:1425	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	9	98	dep	myristoleic	1409:1419	arg1	myristic					1431:1438	myristic	1431:1438	myristic	1431:1438	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	9	99	theme	mice	1498:1501	arg1	liver					1473:1477	liver	1473:1477	liver	1473:1477	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	9	99	theme	mice	1498:1501	arg1	plasma					1484:1489	plasma	1484:1489	plasma	1484:1489	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	9	99	theme	mice	1498:1501	arg1	intestine					1462:1470	intestine	1462:1470	intestine	1462:1470	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	8	100	theme	diacylglycerols	1273:1287	arg1	accumulation					1257:1268	hepatic accumulation	1249:1268	hepatic accumulation of diacylglycerols	1249:1287	Additionally, hepatic accumulation of diacylglycerols is significantly reduced in iKO mice.					
35718096	7	101	theme	cholesterol	1193:1203	arg1	esters					1205:1210	cholesterol esters	1193:1210	cholesterol esters	1193:1210	These mice have significant reductions not only in intestinal lipids, but also in plasma triacylglycerols, diacylglycerols, cholesterol esters, and free cholesterol.					
35718096	5	102	from	intestine	872:880	arg1	enriched					843:850	enriched	843:850	enriched	843:850	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	4	103	theme	impact	693:698	arg1	regulation					656:665	the regulation	652:665	the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues	652:754	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	7	104	contain	have	1080:1083	arg2	reductions					1097:1106	significant reductions	1085:1106	significant reductions	1085:1106	These mice have significant reductions not only in intestinal lipids, but also in plasma triacylglycerols, diacylglycerols, cholesterol esters, and free cholesterol.					
35718096	7	104	contain	have	1080:1083	arg1	mice					1075:1078	These mice	1069:1078	These mice	1069:1078	These mice have significant reductions not only in intestinal lipids, but also in plasma triacylglycerols, diacylglycerols, cholesterol esters, and free cholesterol.					
35718096	0	105	theme	systemic	78:85	arg1	homeostasis					106:116	systemic postprandial lipid homeostasis	78:116	systemic postprandial lipid homeostasis	78:116	SCD1 is nutritionally and spatially regulated in the intestine and influences systemic postprandial lipid homeostasis and gut-liver crosstalk.					
35718096	15	106	theme	important	2343:2351	arg1	implications					2353:2364	important implications	2343:2364	important implications	2343:2364	These data have important implications to understanding how intestinal SCD1 may modulate risk for post-prandial lipemia, hepatic steatosis, and related pathologies.					
35718096	4	107	theme	lipid	703:707	arg1	composition					709:719	lipid composition	703:719	lipid composition	703:719	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	14	108	theme	intestinal	2185:2194	arg1	SCD1					2196:2199	intestinal SCD1	2185:2199	intestinal SCD1	2185:2199	Further, these data point to intestinal SCD1 as a modulator of gut-liver crosstalk, potentially through the production of novel signaling lipids such as myristoleic acid.					
35718096	0	109	theme	lipid	100:104	arg1	homeostasis					106:116	systemic postprandial lipid homeostasis	78:116	systemic postprandial lipid homeostasis	78:116	SCD1 is nutritionally and spatially regulated in the intestine and influences systemic postprandial lipid homeostasis and gut-liver crosstalk.					
35718096	5	110	from	colon	893:897	arg1	enriched					843:850	enriched	843:850	enriched	843:850	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	5	111	theme	acute	947:951	arg1	refeeding					953:961	acute refeeding	947:961	acute refeeding of a high-sucrose diet	947:984	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	9	112	theme	acid	1447:1450	arg1	ratios					1452:1457	the myristoleic (14:1) to myristic (14:0) acid ratios	1405:1457	the myristoleic (14:1) to myristic (14:0) acid ratios	1405:1457	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	6	113	theme	iKO	1058:1060	arg1	intestine					1047:1055	the intestine	1043:1055	the intestine (iKO mice)	1043:1066	We generated a mouse model lacking SCD1 specifically in the intestine (iKO mice).					
35718096	6	113	theme	iKO	1058:1060	arg1	mice					1062:1065	iKO mice	1058:1065	iKO mice	1058:1065	We generated a mouse model lacking SCD1 specifically in the intestine (iKO mice).					
35718096	10	114	theme	fed	1611:1613	arg1	mice					1619:1622	chow fed iKO mice	1606:1622	chow fed iKO mice	1606:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	5	115	theme	other	810:814	arg1	enzymes					826:832	other lipogenic enzymes	810:832	other lipogenic enzymes	810:832	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	11	116	theme	genes	1809:1813	arg1	expression					1777:1786	expression	1777:1786	expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet	1777:1910	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	15	117	theme	post-prandial	2425:2437	arg1	lipemia					2439:2445	post-prandial lipemia	2425:2445	post-prandial lipemia	2425:2445	These data have important implications to understanding how intestinal SCD1 may modulate risk for post-prandial lipemia, hepatic steatosis, and related pathologies.					
35718096	4	118	theme	other	742:746	arg1	tissues					748:754	other tissues	742:754	other tissues	742:754	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35718096	12	119	from	intestine	1995:2003	arg1	pattern					1958:1964	a novel pattern	1950:1964	a novel pattern of expression of SCD1 in the intestine	1950:2003	Taken together, these studies reveal a novel pattern of expression of SCD1 in the intestine.					
35718096	3	120	dep	esterified	536:545	arg1	tissue					529:534	tissue	529:534	tissue	529:534	Specifically, lack of hepatic or cutaneous SCD1 results in significant reductions in tissue esterified lipids.					
35718096	10	121	theme	hepatic	1625:1631	arg1	expression					1633:1642	hepatic expression	1625:1642	hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes	1625:1699	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	9	122	theme	targeted	1341:1348	arg1	profiling					1360:1368	Comprehensive targeted lipidomic profiling	1327:1368	Comprehensive targeted lipidomic profiling	1327:1368	Comprehensive targeted lipidomic profiling revealed a consistent reduction in the myristoleic (14:1) to myristic (14:0) acid ratios in intestine, liver, and plasma of iKO mice.					
35718096	10	123	theme	mice	1619:1622	arg1	lipids					1596:1601	hepatic lipids	1588:1601	hepatic lipids of chow fed iKO mice	1588:1622	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	1	124	theme	monounsaturated	310:324	arg1	counterparts					326:337	their monounsaturated counterparts	304:337	their monounsaturated counterparts	304:337	Stearoyl-CoA desaturase-1 is an endoplasmic reticulum (ER)-membrane resident protein that inserts a double bond into saturated fatty acids, converting them into their monounsaturated counterparts.					
35718096	11	125	theme	de	1791:1792	arg1	genes					1809:1813	de novo lipogenic genes	1791:1813	de novo lipogenic genes	1791:1813	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	5	126	theme	high-sucrose	968:979	arg1	diet					981:984	a high-sucrose diet	966:984	a high-sucrose diet	966:984	Here we report that unlike other lipogenic enzymes, SCD1 is enriched in the distal small intestine and in the colon of chow-fed mice and is robustly upregulated by acute refeeding of a high-sucrose diet.					
35718096	2	127	theme	Previous	340:347	arg1	studies					349:355	Previous studies	340:355	Previous studies	340:355	Previous studies have demonstrated an important role for SCD1 in modulating tissue and systemic health.					
35718096	15	128	theme	related	2471:2477	arg1	pathologies					2479:2489	related pathologies	2471:2489	related pathologies	2471:2489	These data have important implications to understanding how intestinal SCD1 may modulate risk for post-prandial lipemia, hepatic steatosis, and related pathologies.					
35718096	7	129	theme	free	1217:1220	arg1	cholesterol					1222:1232	free cholesterol	1217:1232	free cholesterol	1217:1232	These mice have significant reductions not only in intestinal lipids, but also in plasma triacylglycerols, diacylglycerols, cholesterol esters, and free cholesterol.					
35718096	11	130	theme	chow-fed	1838:1845	arg1	mice					1847:1850	chow-fed mice	1838:1850	chow-fed mice	1838:1850	Further, lack of intestinal SCD1 reduced expression of de novo lipogenic genes in distal intestine of chow-fed mice and in the livers of mice fed a lipogenic high-sucrose diet.					
35718096	14	131	theme	lipids	2294:2299	arg1	production					2264:2273	the production	2260:2273	the production of novel signaling lipids such as myristoleic acid	2260:2324	Further, these data point to intestinal SCD1 as a modulator of gut-liver crosstalk, potentially through the production of novel signaling lipids such as myristoleic acid.					
35718096	10	132	theme	related	1666:1672	arg1	oxidation					1685:1693	related fatty acid oxidation	1666:1693	related fatty acid oxidation	1666:1693	Consistent with the reduction of the monounsaturated fatty acid myristoleic acid in hepatic lipids of chow fed iKO mice, hepatic expression of Pgc-1α, Sirt1, and related fatty acid oxidation genes were reduced in chow-fed iKO mice.					
35718096	4	133	from	composition	709:719	arg1	regulation					656:665	the regulation	652:665	the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues	652:754	While the intestine is an important site of lipid esterification and assimilation into the body, the regulation of intestinal SCD1 or its impact on lipid composition in the intestine and other tissues has not been investigated.					
35275402	12	0	theme	fruit	1820:1824	arg1	juice					1826:1830	papaya fruit juice	1813:1830	papaya fruit juice	1813:1830	Acidified blanching and pectinase enzyme pretreatments have an impact on extraction of papaya fruit juice while retaining the nutritional composition of the juice.					
35275402	7	1	theme	soluble	1014:1020	arg1	solids					1022:1027	total soluble solids	1008:1027	total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC]	1008:1090	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	3	2	contain	containing	547:556	arg1	water					541:545	water	541:545	water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min	541:614	For acidified blanching treatment, papaya cubes (3 cm3 ) were blanched in water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min.					
35275402	3	2	contain	containing	547:556	arg2	%					565:565	0.5%	562:565	0.5%	562:565	For acidified blanching treatment, papaya cubes (3 cm3 ) were blanched in water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min.					
35275402	3	2	contain	containing	547:556	arg2	%					559:559	0%	558:559	0%	558:559	For acidified blanching treatment, papaya cubes (3 cm3 ) were blanched in water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min.					
35275402	3	2	contain	containing	547:556	arg2	%					581:581	2.5%	578:581	2.5%	578:581	For acidified blanching treatment, papaya cubes (3 cm3 ) were blanched in water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min.					
35275402	3	2	contain	containing	547:556	arg2	%					571:571	1.5%	568:571	1.5%	568:571	For acidified blanching treatment, papaya cubes (3 cm3 ) were blanched in water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min.					
35275402	7	3	theme	papaya	918:923	arg1	juice					925:929	All pretreated papaya juice	903:929	All pretreated papaya juice	903:929	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	2	4	theme	acidified	316:324	arg1	water					336:340	acidified blanching water and pectinase enzyme pretreatments	316:375	water	336:340	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	9	5	theme	juice	1421:1425	arg1	TPC					1380:1382	TPC	1380:1382	TPC	1380:1382	The TPC and antioxidant activities of papaya juice were conserved and maintained by acidified blanching at 1.5% (w/v) citric acid.					
35275402	9	5	theme	juice	1421:1425	arg1	activities					1400:1409	antioxidant activities	1388:1409	antioxidant activities	1388:1409	The TPC and antioxidant activities of papaya juice were conserved and maintained by acidified blanching at 1.5% (w/v) citric acid.					
35275402	3	6	theme	blanching	481:489	arg1	treatment					491:499	acidified blanching treatment	471:499	acidified blanching treatment	471:499	For acidified blanching treatment, papaya cubes (3 cm3 ) were blanched in water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min.					
35275402	12	7	from	impact	1789:1794	arg1	extraction					1799:1808	extraction	1799:1808	extraction of papaya fruit juice	1799:1830	Acidified blanching and pectinase enzyme pretreatments have an impact on extraction of papaya fruit juice while retaining the nutritional composition of the juice.					
35275402	2	8	from	effect	306:311	arg1	yield					384:388	the yield	380:388	the yield	380:388	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	2	8	from	effect	306:311	arg1	properties					407:416	physicochemical properties	391:416	physicochemical properties	391:416	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	2	8	from	effect	306:311	arg1	activities					435:444	antioxidant activities	423:444	antioxidant activities of the papaya juice	423:464	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	11	9	with	Pretreatment	1612:1623	arg1	enzyme					1640:1645	pectinase enzyme	1630:1645	pectinase enzyme	1630:1645	Pretreatment with pectinase enzyme up to 20 ppm does not significantly reduce the TFC and antioxidant activities.					
35275402	12	10	theme	pectinase	1750:1758	arg1	pretreatments					1767:1779	Acidified blanching and pectinase enzyme pretreatments	1726:1779	Acidified blanching and pectinase enzyme pretreatments	1726:1779	Acidified blanching and pectinase enzyme pretreatments have an impact on extraction of papaya fruit juice while retaining the nutritional composition of the juice.					
35275402	7	11	theme	antioxidant	1214:1224	arg1	[FRAP					1232:1236	ferric reducing antioxidant power [FRAP	1198:1236	ferric reducing antioxidant power [FRAP] assay)	1198:1244	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	4	12	theme	papaya	707:712	arg1	puree					714:718	the homogenous papaya puree	692:718	the homogenous papaya puree	692:718	For enzyme treatment, a pectinase enzyme (10, 20, and 30 ppm) was added to the homogenous papaya puree and incubated at 45℃ (200 rpm) for 60 min.					
35275402	10	13	theme	pectinase	1535:1543	arg1	enzyme					1545:1550	pectinase enzyme	1535:1550	pectinase enzyme	1535:1550	Increased concentrations of pectinase enzyme significantly reduced the TPC and total carotenoid content.					
35275402	3	14	dep	%	559:559	arg1	acid					594:597	w/v citric acid	583:597	w/v citric acid	583:597	For acidified blanching treatment, papaya cubes (3 cm3 ) were blanched in water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min.					
35275402	9	15	theme	acidified	1460:1468	arg1	blanching					1470:1478	acidified blanching	1460:1478	acidified blanching	1460:1478	The TPC and antioxidant activities of papaya juice were conserved and maintained by acidified blanching at 1.5% (w/v) citric acid.					
35275402	7	16	theme	antioxidant	1133:1143	arg1	activities					1145:1154	antioxidant activities	1133:1154	antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH]	1133:1192	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	16	theme	antioxidant	1133:1143	arg1	[DPPH					1187:1191	2,2-diphenyl-1-picrylhydrazyl [DPPH	1157:1191	2,2-diphenyl-1-picrylhydrazyl [DPPH	1157:1191	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	17	dep	solids	1022:1027	arg1	[TSSs					1029:1033	[TSSs	1029:1033	[TSSs	1029:1033	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	0	18	theme	Carica	130:135	arg1	juice					144:148	Carica papaya juice	130:148	Carica papaya juice	130:148	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	9	19	dep	TPC	1380:1382	arg1	The					1376:1378	The	1376:1378	The	1376:1378	The TPC and antioxidant activities of papaya juice were conserved and maintained by acidified blanching at 1.5% (w/v) citric acid.					
35275402	0	20	theme	juice	144:148	arg1	properties					91:100	physicochemical properties	75:100	physicochemical properties	75:100	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	0	20	theme	juice	144:148	arg1	capacity					118:125	antioxidant capacity	106:125	antioxidant capacity	106:125	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	7	21	theme	physicochemical	949:963	arg1	viscosity					993:1001	viscosity	993:1001	viscosity	993:1001	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	21	theme	physicochemical	949:963	arg1	properties					965:974	physicochemical properties	949:974	physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC])	949:1126	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	21	theme	physicochemical	949:963	arg1	color					977:981	color	977:981	color	977:981	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	21	theme	physicochemical	949:963	arg1	pH					1004:1005	pH	1004:1005	pH	1004:1005	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	21	theme	physicochemical	949:963	arg1	[TFC					1121:1124	total flavonoid content [TFC	1097:1124	total flavonoid content [TFC	1097:1124	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	21	theme	physicochemical	949:963	arg1	clarity					984:990	clarity	984:990	clarity	984:990	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	12	22	theme	blanching	1736:1744	arg1	pretreatments					1767:1779	Acidified blanching and pectinase enzyme pretreatments	1726:1779	Acidified blanching and pectinase enzyme pretreatments	1726:1779	Acidified blanching and pectinase enzyme pretreatments have an impact on extraction of papaya fruit juice while retaining the nutritional composition of the juice.					
35275402	9	23	theme	%	1486:1486	arg1	acid					1501:1504	1.5% (w/v) citric acid	1483:1504	1.5% (w/v) citric acid	1483:1504	The TPC and antioxidant activities of papaya juice were conserved and maintained by acidified blanching at 1.5% (w/v) citric acid.					
35275402	16	24	dep	blanching	2355:2363	arg1	clarify					2389:2395	clarify	2389:2395	to clarify the juice	2386:2405	Using acidified blanching and pectinase enzyme to clarify the juice not only increases the yield but also retains the nutrient composition of the juice.					
35275402	16	25	theme	juice	2485:2489	arg1	composition					2466:2476	the nutrient composition	2453:2476	the nutrient composition of the juice	2453:2489	Using acidified blanching and pectinase enzyme to clarify the juice not only increases the yield but also retains the nutrient composition of the juice.					
35275402	7	26	theme	content	1113:1119	arg1	properties					965:974	physicochemical properties	949:974	physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC])	949:1126	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	26	theme	content	1113:1119	arg1	[TFC					1121:1124	total flavonoid content [TFC	1097:1124	total flavonoid content [TFC	1097:1124	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	13	27	theme	PRACTICAL	1890:1898	arg1	APPLICATION					1900:1910	PRACTICAL APPLICATION	1890:1910	PRACTICAL APPLICATION: Papaya (Carica papaya) is a fruit with great nutritional values but is highly perishable and prone to postharvest loss.	1890:2031	PRACTICAL APPLICATION: Papaya (Carica papaya) is a fruit with great nutritional values but is highly perishable and prone to postharvest loss.					
35275402	8	28	with	pretreatment	1340:1351	arg1	enzyme					1368:1373	pectinase enzyme	1358:1373	pectinase enzyme	1358:1373	Both pretreatments improved the clarity of papaya juice, and was significantly greater after pretreatment with pectinase enzyme.					
35275402	9	29	theme	citric	1494:1499	arg1	acid					1501:1504	1.5% (w/v) citric acid	1483:1504	1.5% (w/v) citric acid	1483:1504	The TPC and antioxidant activities of papaya juice were conserved and maintained by acidified blanching at 1.5% (w/v) citric acid.					
35275402	7	30	theme	total	1097:1101	arg1	content					1113:1119	total flavonoid content	1097:1119	total flavonoid content [TFC	1097:1124	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	31	theme	total	1008:1012	arg1	solids					1022:1027	total soluble solids	1008:1027	total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC]	1008:1090	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	1	32	theme	high	155:158	arg1	content					167:173	The high pectin content	151:173	The high pectin content in papaya (Carica papaya)	151:199	The high pectin content in papaya (Carica papaya) causes the juice extraction to be difficult and results in a low yield.					
35275402	14	33	theme	convenient	2076:2085	arg1	option					2087:2092	a more cost-effective and convenient option	2050:2092	a more cost-effective and convenient option for preserving the fruit	2050:2117	Juice has become a more cost-effective and convenient option for preserving the fruit.					
35275402	0	34	theme	physicochemical	75:89	arg1	properties					91:100	physicochemical properties	75:100	physicochemical properties	75:100	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	0	35	from	Effects	0:6	arg1	properties					91:100	physicochemical properties	75:100	physicochemical properties	75:100	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	0	35	from	Effects	0:6	arg1	capacity					118:125	antioxidant capacity	106:125	antioxidant capacity	106:125	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	14	36	theme	cost-effective	2057:2070	arg1	option					2087:2092	a more cost-effective and convenient option	2050:2092	a more cost-effective and convenient option for preserving the fruit	2050:2117	Juice has become a more cost-effective and convenient option for preserving the fruit.					
35275402	7	37	theme	ferric	1198:1203	arg1	[FRAP					1232:1236	ferric reducing antioxidant power [FRAP	1198:1236	ferric reducing antioxidant power [FRAP] assay)	1198:1244	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	2	38	theme	antioxidant	423:433	arg1	activities					435:444	antioxidant activities	423:444	antioxidant activities of the papaya juice	423:464	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	8	39	theme	papaya	1290:1295	arg1	juice					1297:1301	papaya juice	1290:1301	papaya juice	1290:1301	Both pretreatments improved the clarity of papaya juice, and was significantly greater after pretreatment with pectinase enzyme.					
35275402	2	40	theme	enzyme	356:361	arg1	pretreatments					363:375	acidified blanching water and pectinase enzyme pretreatments	316:375	pretreatments	363:375	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	15	41	theme	juice	2251:2255	arg1	bonding					2240:2246	the bonding	2236:2246	the bonding of juice to the pulp in the form of a jellied mass, which results in juice with low yield	2236:2336	However, since papaya is a pectin-rich fruit, the extraction of juice using mechanical pressing is difficult due to the bonding of juice to the pulp in the form of a jellied mass, which results in juice with low yield.					
35275402	5	42	theme	enzyme	767:772	arg1	reaction					774:781	The enzyme reaction	763:781	The enzyme reaction	763:781	The enzyme reaction was stopped by pasteurization at 74℃ for 2 min.					
35275402	2	43	theme	physicochemical	391:405	arg1	properties					407:416	physicochemical properties	391:416	physicochemical properties	391:416	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	0	44	theme	acidified	11:19	arg1	water					31:35	acidified blanching water	11:35	acidified blanching water	11:35	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	11	45	dep	20 ppm	1653:1658	arg1	up					1647:1648	up	1647:1648	up	1647:1648	Pretreatment with pectinase enzyme up to 20 ppm does not significantly reduce the TFC and antioxidant activities.					
35275402	7	46	theme	content	1078:1084	arg1	[TPC					1086:1089	total phenolic content [TPC	1063:1089	total phenolic content [TPC	1063:1089	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	2	47	theme	papaya	453:458	arg1	juice					460:464	the papaya juice	449:464	the papaya juice	449:464	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	0	48	theme	water	31:35	arg1	Effects					0:6	Effects	0:6	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.	0:149	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	7	49	theme	total	1063:1067	arg1	content					1078:1084	total phenolic content	1063:1084	total phenolic content [TPC	1063:1089	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	0	50	theme	pretreatments	58:70	arg1	Effects					0:6	Effects	0:6	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.	0:149	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	0	51	theme	pectinase	41:49	arg1	pretreatments					58:70	pectinase enzyme pretreatments	41:70	pectinase enzyme pretreatments	41:70	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	16	52	theme	pectinase	2369:2377	arg1	enzyme					2379:2384	pectinase enzyme	2369:2384	pectinase enzyme	2369:2384	Using acidified blanching and pectinase enzyme to clarify the juice not only increases the yield but also retains the nutrient composition of the juice.					
35275402	3	53	theme	citric	587:592	arg1	acid					594:597	w/v citric acid	583:597	w/v citric acid	583:597	For acidified blanching treatment, papaya cubes (3 cm3 ) were blanched in water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min.					
35275402	1	54	theme	juice	212:216	arg1	extraction					218:227	the juice extraction	208:227	the juice extraction	208:227	The high pectin content in papaya (Carica papaya) causes the juice extraction to be difficult and results in a low yield.					
35275402	1	54	theme	juice	212:216	arg1	difficult					235:243	difficult	235:243	difficult	235:243	The high pectin content in papaya (Carica papaya) causes the juice extraction to be difficult and results in a low yield.					
35275402	7	55	theme	total	1037:1041	arg1	content					1054:1060	total carotenoid content	1037:1060	total carotenoid content	1037:1060	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	8	56	theme	pectinase	1358:1366	arg1	enzyme					1368:1373	pectinase enzyme	1358:1373	pectinase enzyme	1358:1373	Both pretreatments improved the clarity of papaya juice, and was significantly greater after pretreatment with pectinase enzyme.					
35275402	13	57	theme	great	1952:1956	arg1	values					1970:1975	great nutritional values	1952:1975	great nutritional values	1952:1975	PRACTICAL APPLICATION: Papaya (Carica papaya) is a fruit with great nutritional values but is highly perishable and prone to postharvest loss.					
35275402	13	58	with	fruit	1941:1945	arg1	values					1970:1975	great nutritional values	1952:1975	great nutritional values	1952:1975	PRACTICAL APPLICATION: Papaya (Carica papaya) is a fruit with great nutritional values but is highly perishable and prone to postharvest loss.					
35275402	10	59	theme	carotenoid	1592:1601	arg1	content					1603:1609	total carotenoid content	1586:1609	total carotenoid content	1586:1609	Increased concentrations of pectinase enzyme significantly reduced the TPC and total carotenoid content.					
35275402	7	60	theme	pretreated	907:916	arg1	juice					925:929	All pretreated papaya juice	903:929	All pretreated papaya juice	903:929	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	15	61	with	juice	2317:2321	arg1	yield					2332:2336	low yield	2328:2336	low yield	2328:2336	However, since papaya is a pectin-rich fruit, the extraction of juice using mechanical pressing is difficult due to the bonding of juice to the pulp in the form of a jellied mass, which results in juice with low yield.					
35275402	2	62	theme	blanching	326:334	arg1	water					336:340	acidified blanching water and pectinase enzyme pretreatments	316:375	water	336:340	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	12	63	theme	papaya	1813:1818	arg1	juice					1826:1830	papaya fruit juice	1813:1830	papaya fruit juice	1813:1830	Acidified blanching and pectinase enzyme pretreatments have an impact on extraction of papaya fruit juice while retaining the nutritional composition of the juice.					
35275402	9	64	theme	papaya	1414:1419	arg1	juice					1421:1425	papaya juice	1414:1425	papaya juice	1414:1425	The TPC and antioxidant activities of papaya juice were conserved and maintained by acidified blanching at 1.5% (w/v) citric acid.					
35275402	13	65	dep	APPLICATION	1900:1910	arg1	Papaya					1913:1918	Papaya	1913:1918	Papaya (Carica papaya)	1913:1934	PRACTICAL APPLICATION: Papaya (Carica papaya) is a fruit with great nutritional values but is highly perishable and prone to postharvest loss.					
35275402	13	65	dep	APPLICATION	1900:1910	arg1	fruit					1941:1945	a fruit	1939:1945	a fruit with great nutritional values	1939:1975	PRACTICAL APPLICATION: Papaya (Carica papaya) is a fruit with great nutritional values but is highly perishable and prone to postharvest loss.					
35275402	13	65	dep	APPLICATION	1900:1910	arg1	perishable					1991:2000	perishable	1991:2000	perishable	1991:2000	PRACTICAL APPLICATION: Papaya (Carica papaya) is a fruit with great nutritional values but is highly perishable and prone to postharvest loss.					
35275402	10	66	theme	Increased	1507:1515	arg1	concentrations					1517:1530	Increased concentrations	1507:1530	Increased concentrations of pectinase enzyme	1507:1550	Increased concentrations of pectinase enzyme significantly reduced the TPC and total carotenoid content.					
35275402	15	67	theme	jellied	2286:2292	arg1	mass					2294:2297	a jellied mass	2284:2297	a jellied mass	2284:2297	However, since papaya is a pectin-rich fruit, the extraction of juice using mechanical pressing is difficult due to the bonding of juice to the pulp in the form of a jellied mass, which results in juice with low yield.					
35275402	7	68	theme	power	1226:1230	arg1	[FRAP					1232:1236	ferric reducing antioxidant power [FRAP	1198:1236	ferric reducing antioxidant power [FRAP] assay)	1198:1244	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	4	69	dep	enzyme	651:656	arg1	20					663:664	20	663:664	20	663:664	For enzyme treatment, a pectinase enzyme (10, 20, and 30 ppm) was added to the homogenous papaya puree and incubated at 45℃ (200 rpm) for 60 min.					
35275402	4	69	dep	enzyme	651:656	arg1	30 ppm					671:676	30 ppm	671:676	30 ppm	671:676	For enzyme treatment, a pectinase enzyme (10, 20, and 30 ppm) was added to the homogenous papaya puree and incubated at 45℃ (200 rpm) for 60 min.					
35275402	12	70	contain	have	1781:1784	arg2	impact					1789:1794	an impact	1786:1794	an impact on extraction of papaya fruit juice	1786:1830	Acidified blanching and pectinase enzyme pretreatments have an impact on extraction of papaya fruit juice while retaining the nutritional composition of the juice.					
35275402	12	70	contain	have	1781:1784	arg1	pretreatments					1767:1779	Acidified blanching and pectinase enzyme pretreatments	1726:1779	Acidified blanching and pectinase enzyme pretreatments	1726:1779	Acidified blanching and pectinase enzyme pretreatments have an impact on extraction of papaya fruit juice while retaining the nutritional composition of the juice.					
35275402	7	71	theme	reducing	1205:1212	arg1	[FRAP					1232:1236	ferric reducing antioxidant power [FRAP	1198:1236	ferric reducing antioxidant power [FRAP] assay)	1198:1244	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	10	72	theme	enzyme	1545:1550	arg1	concentrations					1517:1530	Increased concentrations	1507:1530	Increased concentrations of pectinase enzyme	1507:1550	Increased concentrations of pectinase enzyme significantly reduced the TPC and total carotenoid content.					
35275402	4	73	theme	homogenous	696:705	arg1	puree					714:718	the homogenous papaya puree	692:718	the homogenous papaya puree	692:718	For enzyme treatment, a pectinase enzyme (10, 20, and 30 ppm) was added to the homogenous papaya puree and incubated at 45℃ (200 rpm) for 60 min.					
35275402	7	74	theme	2,2-diphenyl-1-picrylhydrazyl	1157:1185	arg1	activities					1145:1154	antioxidant activities	1133:1154	antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH]	1133:1192	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	74	theme	2,2-diphenyl-1-picrylhydrazyl	1157:1185	arg1	[DPPH					1187:1191	2,2-diphenyl-1-picrylhydrazyl [DPPH	1157:1191	2,2-diphenyl-1-picrylhydrazyl [DPPH	1157:1191	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	0	75	theme	papaya	137:142	arg1	juice					144:148	Carica papaya juice	130:148	Carica papaya juice	130:148	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	12	76	theme	enzyme	1760:1765	arg1	pretreatments					1767:1779	Acidified blanching and pectinase enzyme pretreatments	1726:1779	Acidified blanching and pectinase enzyme pretreatments	1726:1779	Acidified blanching and pectinase enzyme pretreatments have an impact on extraction of papaya fruit juice while retaining the nutritional composition of the juice.					
35275402	12	77	theme	juice	1883:1887	arg1	composition					1864:1874	the nutritional composition	1848:1874	the nutritional composition of the juice	1848:1887	Acidified blanching and pectinase enzyme pretreatments have an impact on extraction of papaya fruit juice while retaining the nutritional composition of the juice.					
35275402	3	78	theme	acidified	471:479	arg1	treatment					491:499	acidified blanching treatment	471:499	acidified blanching treatment	471:499	For acidified blanching treatment, papaya cubes (3 cm3 ) were blanched in water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min.					
35275402	15	79	theme	pectin-rich	2147:2157	arg1	fruit					2159:2163	a pectin-rich fruit	2145:2163	a pectin-rich fruit	2145:2163	However, since papaya is a pectin-rich fruit, the extraction of juice using mechanical pressing is difficult due to the bonding of juice to the pulp in the form of a jellied mass, which results in juice with low yield.					
35275402	15	79	theme	pectin-rich	2147:2157	arg1	papaya					2135:2140	papaya	2135:2140	papaya	2135:2140	However, since papaya is a pectin-rich fruit, the extraction of juice using mechanical pressing is difficult due to the bonding of juice to the pulp in the form of a jellied mass, which results in juice with low yield.					
35275402	1	80	theme	Carica	186:191	arg1	papaya					178:183	papaya	178:183	papaya (Carica papaya)	178:199	The high pectin content in papaya (Carica papaya) causes the juice extraction to be difficult and results in a low yield.					
35275402	1	80	theme	Carica	186:191	arg1	papaya					193:198	Carica papaya	186:198	Carica papaya	186:198	The high pectin content in papaya (Carica papaya) causes the juice extraction to be difficult and results in a low yield.					
35275402	16	81	theme	acidified	2345:2353	arg1	blanching					2355:2363	acidified blanching	2345:2363	acidified blanching	2345:2363	Using acidified blanching and pectinase enzyme to clarify the juice not only increases the yield but also retains the nutrient composition of the juice.					
35275402	9	82	theme	1.5	1483:1485	arg1	%					1486:1486	%	1486:1486	%	1486:1486	The TPC and antioxidant activities of papaya juice were conserved and maintained by acidified blanching at 1.5% (w/v) citric acid.					
35275402	13	83	theme	Carica	1921:1926	arg1	Papaya					1913:1918	Papaya	1913:1918	Papaya (Carica papaya)	1913:1934	PRACTICAL APPLICATION: Papaya (Carica papaya) is a fruit with great nutritional values but is highly perishable and prone to postharvest loss.					
35275402	13	83	theme	Carica	1921:1926	arg1	papaya					1928:1933	Carica papaya	1921:1933	Carica papaya	1921:1933	PRACTICAL APPLICATION: Papaya (Carica papaya) is a fruit with great nutritional values but is highly perishable and prone to postharvest loss.					
35275402	12	84	theme	Acidified	1726:1734	arg1	pretreatments					1767:1779	Acidified blanching and pectinase enzyme pretreatments	1726:1779	Acidified blanching and pectinase enzyme pretreatments	1726:1779	Acidified blanching and pectinase enzyme pretreatments have an impact on extraction of papaya fruit juice while retaining the nutritional composition of the juice.					
35275402	12	85	theme	nutritional	1852:1862	arg1	composition					1864:1874	the nutritional composition	1848:1874	the nutritional composition of the juice	1848:1887	Acidified blanching and pectinase enzyme pretreatments have an impact on extraction of papaya fruit juice while retaining the nutritional composition of the juice.					
35275402	15	86	from	pulp	2264:2267	arg1	form					2276:2279	the form	2272:2279	the form of a jellied mass, which results in juice with low yield	2272:2336	However, since papaya is a pectin-rich fruit, the extraction of juice using mechanical pressing is difficult due to the bonding of juice to the pulp in the form of a jellied mass, which results in juice with low yield.					
35275402	10	87	theme	total	1586:1590	arg1	content					1603:1609	total carotenoid content	1586:1609	total carotenoid content	1586:1609	Increased concentrations of pectinase enzyme significantly reduced the TPC and total carotenoid content.					
35275402	13	88	theme	postharvest	2015:2025	arg1	loss					2027:2030	postharvest loss	2015:2030	postharvest loss	2015:2030	PRACTICAL APPLICATION: Papaya (Carica papaya) is a fruit with great nutritional values but is highly perishable and prone to postharvest loss.					
35275402	7	89	theme	flavonoid	1103:1111	arg1	content					1113:1119	total flavonoid content	1097:1119	total flavonoid content [TFC	1097:1124	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	12	90	theme	juice	1826:1830	arg1	extraction					1799:1808	extraction	1799:1808	extraction of papaya fruit juice	1799:1830	Acidified blanching and pectinase enzyme pretreatments have an impact on extraction of papaya fruit juice while retaining the nutritional composition of the juice.					
35275402	15	91	theme	mechanical	2196:2205	arg1	pressing					2207:2214	mechanical pressing	2196:2214	mechanical pressing	2196:2214	However, since papaya is a pectin-rich fruit, the extraction of juice using mechanical pressing is difficult due to the bonding of juice to the pulp in the form of a jellied mass, which results in juice with low yield.					
35275402	1	92	theme	pectin	160:165	arg1	content					167:173	The high pectin content	151:173	The high pectin content in papaya (Carica papaya)	151:199	The high pectin content in papaya (Carica papaya) causes the juice extraction to be difficult and results in a low yield.					
35275402	2	93	theme	juice	460:464	arg1	yield					384:388	the yield	380:388	the yield	380:388	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	2	93	theme	juice	460:464	arg1	properties					407:416	physicochemical properties	391:416	physicochemical properties	391:416	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	2	93	theme	juice	460:464	arg1	activities					435:444	antioxidant activities	423:444	antioxidant activities of the papaya juice	423:464	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	7	94	dep	properties	965:974	arg1	viscosity					993:1001	viscosity	993:1001	viscosity	993:1001	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	94	dep	properties	965:974	arg1	solids					1022:1027	total soluble solids	1008:1027	total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC]	1008:1090	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	94	dep	properties	965:974	arg1	properties					965:974	physicochemical properties	949:974	physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC])	949:1126	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	94	dep	properties	965:974	arg1	color					977:981	color	977:981	color	977:981	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	94	dep	properties	965:974	arg1	pH					1004:1005	pH	1004:1005	pH	1004:1005	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	94	dep	properties	965:974	arg1	[TFC					1121:1124	total flavonoid content [TFC	1097:1124	total flavonoid content [TFC	1097:1124	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	94	dep	properties	965:974	arg1	clarity					984:990	clarity	984:990	clarity	984:990	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	0	95	theme	antioxidant	106:116	arg1	capacity					118:125	antioxidant capacity	106:125	antioxidant capacity	106:125	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	15	96	theme	juice	2184:2188	arg1	difficult					2219:2227	difficult	2219:2227	difficult	2219:2227	However, since papaya is a pectin-rich fruit, the extraction of juice using mechanical pressing is difficult due to the bonding of juice to the pulp in the form of a jellied mass, which results in juice with low yield.					
35275402	15	96	theme	juice	2184:2188	arg1	extraction					2170:2179	the extraction	2166:2179	the extraction of juice using mechanical pressing	2166:2214	However, since papaya is a pectin-rich fruit, the extraction of juice using mechanical pressing is difficult due to the bonding of juice to the pulp in the form of a jellied mass, which results in juice with low yield.					
35275402	1	97	from	content	167:173	arg1	papaya					178:183	papaya	178:183	papaya (Carica papaya)	178:199	The high pectin content in papaya (Carica papaya) causes the juice extraction to be difficult and results in a low yield.					
35275402	1	97	from	content	167:173	arg1	papaya					193:198	Carica papaya	186:198	Carica papaya	186:198	The high pectin content in papaya (Carica papaya) causes the juice extraction to be difficult and results in a low yield.					
35275402	4	98	theme	pectinase	641:649	arg1	enzyme					651:656	a pectinase enzyme	639:656	a pectinase enzyme (10, 20, and 30 ppm)	639:677	For enzyme treatment, a pectinase enzyme (10, 20, and 30 ppm) was added to the homogenous papaya puree and incubated at 45℃ (200 rpm) for 60 min.					
35275402	9	99	dep	%	1486:1486	arg1	w/v					1489:1491	w/v	1489:1491	w/v	1489:1491	The TPC and antioxidant activities of papaya juice were conserved and maintained by acidified blanching at 1.5% (w/v) citric acid.					
35275402	8	100	theme	juice	1297:1301	arg1	clarity					1279:1285	the clarity	1275:1285	the clarity of papaya juice	1275:1301	Both pretreatments improved the clarity of papaya juice, and was significantly greater after pretreatment with pectinase enzyme.					
35275402	2	101	theme	pretreatments	363:375	arg1	effect					306:311	the effect	302:311	the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice	302:464	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	4	102	theme	enzyme	621:626	arg1	treatment					628:636	enzyme treatment	621:636	enzyme treatment	621:636	For enzyme treatment, a pectinase enzyme (10, 20, and 30 ppm) was added to the homogenous papaya puree and incubated at 45℃ (200 rpm) for 60 min.					
35275402	2	103	theme	pectinase	346:354	arg1	pretreatments					363:375	acidified blanching water and pectinase enzyme pretreatments	316:375	pretreatments	363:375	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	0	104	theme	blanching	21:29	arg1	water					31:35	acidified blanching water	11:35	acidified blanching water	11:35	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	1	105	theme	low	262:264	arg1	yield					266:270	a low yield	260:270	a low yield	260:270	The high pectin content in papaya (Carica papaya) causes the juice extraction to be difficult and results in a low yield.					
35275402	7	106	dep	[TSSs	1029:1033	arg1	[TPC					1086:1089	total phenolic content [TPC	1063:1089	total phenolic content [TPC	1063:1089	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	106	dep	[TSSs	1029:1033	arg1	content					1054:1060	total carotenoid content	1037:1060	total carotenoid content	1037:1060	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	7	107	theme	phenolic	1069:1076	arg1	content					1078:1084	total phenolic content	1063:1084	total phenolic content [TPC	1063:1089	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	15	108	theme	low	2328:2330	arg1	yield					2332:2336	low yield	2328:2336	low yield	2328:2336	However, since papaya is a pectin-rich fruit, the extraction of juice using mechanical pressing is difficult due to the bonding of juice to the pulp in the form of a jellied mass, which results in juice with low yield.					
35275402	11	109	theme	antioxidant	1702:1712	arg1	activities					1714:1723	antioxidant activities	1702:1723	antioxidant activities	1702:1723	Pretreatment with pectinase enzyme up to 20 ppm does not significantly reduce the TFC and antioxidant activities.					
35275402	16	110	theme	nutrient	2457:2464	arg1	composition					2466:2476	the nutrient composition	2453:2476	the nutrient composition of the juice	2453:2489	Using acidified blanching and pectinase enzyme to clarify the juice not only increases the yield but also retains the nutrient composition of the juice.					
35275402	0	111	theme	enzyme	51:56	arg1	pretreatments					58:70	pectinase enzyme pretreatments	41:70	pectinase enzyme pretreatments	41:70	Effects of acidified blanching water and pectinase enzyme pretreatments on physicochemical properties and antioxidant capacity of Carica papaya juice.					
35275402	7	112	theme	carotenoid	1043:1052	arg1	content					1054:1060	total carotenoid content	1037:1060	total carotenoid content	1037:1060	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	3	113	theme	w/v	583:585	arg1	acid					594:597	w/v citric acid	583:597	w/v citric acid	583:597	For acidified blanching treatment, papaya cubes (3 cm3 ) were blanched in water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min.					
35275402	11	114	theme	pectinase	1630:1638	arg1	enzyme					1640:1645	pectinase enzyme	1630:1645	pectinase enzyme	1630:1645	Pretreatment with pectinase enzyme up to 20 ppm does not significantly reduce the TFC and antioxidant activities.					
35275402	7	115	dep	[FRAP	1232:1236	arg1	assay					1239:1243	assay	1239:1243	assay	1239:1243	All pretreated papaya juice were analyzed for physicochemical properties (color, clarity, viscosity, pH, total soluble solids [TSSs], total carotenoid content, total phenolic content [TPC], and total flavonoid content [TFC]), and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] assay).					
35275402	2	116	theme	water	336:340	arg1	effect					306:311	the effect	302:311	the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice	302:464	This study aims to determine the effect of acidified blanching water and pectinase enzyme pretreatments on the yield, physicochemical properties, and antioxidant activities of the papaya juice.					
35275402	9	117	theme	antioxidant	1388:1398	arg1	activities					1400:1409	antioxidant activities	1388:1409	antioxidant activities	1388:1409	The TPC and antioxidant activities of papaya juice were conserved and maintained by acidified blanching at 1.5% (w/v) citric acid.					
35275402	3	118	theme	papaya	502:507	arg1	cubes					509:513	papaya cubes	502:513	papaya cubes (3 cm3 )	502:522	For acidified blanching treatment, papaya cubes (3 cm3 ) were blanched in water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min.					
35275402	3	118	theme	papaya	502:507	arg1	cm3					518:520	3 cm3	516:520	3 cm3	516:520	For acidified blanching treatment, papaya cubes (3 cm3 ) were blanched in water containing 0%, 0.5%, 1.5%, and 2.5% w/v citric acid at 95℃ for 2 min.					
35275402	15	119	theme	mass	2294:2297	arg1	form					2276:2279	the form	2272:2279	the form of a jellied mass, which results in juice with low yield	2272:2336	However, since papaya is a pectin-rich fruit, the extraction of juice using mechanical pressing is difficult due to the bonding of juice to the pulp in the form of a jellied mass, which results in juice with low yield.					
35275402	13	120	theme	nutritional	1958:1968	arg1	values					1970:1975	great nutritional values	1952:1975	great nutritional values	1952:1975	PRACTICAL APPLICATION: Papaya (Carica papaya) is a fruit with great nutritional values but is highly perishable and prone to postharvest loss.					
35064103	7	0	theme	gene	1266:1269	arg1	knockdown					1271:1279	gene knockdown	1266:1279	gene knockdown of Nfatc4 gene by bilateral injection of adeno-associated virus (AAV) into the mPFC	1266:1363	Similar to (R)-ketamine, gene knockdown of Nfatc4 gene by bilateral injection of adeno-associated virus (AAV) into the mPFC could elicit prophylactic effects in the LPS-treated mice.					
35064103	4	1	theme	subsequent	722:731	arg1	Analysis					756:763	Ingenuity Pathway Analysis	738:763	Ingenuity Pathway Analysis	738:763	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	4	1	theme	subsequent	722:731	arg1	IPA					733:735	subsequent IPA	722:735	subsequent IPA (Ingenuity Pathway Analysis)	722:764	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	3	2	from	splenomegaly	546:557	arg1	mice					661:664	lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice	617:664	lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice	617:664	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	5	3	from	expression	1001:1010	arg1	PFC					1075:1077	the PFC	1071:1077	the PFC of LPS-treated mice	1071:1097	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	2	4	dep	-ketamine	434:442	arg1	R					429:429	R	429:429	R	429:429	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	2	4	dep	-ketamine	434:442	arg1	S					432:432	S	432:432	S	432:432	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	4	5	theme	R	896:896	arg1	-ketamine					898:906	(R)-ketamine	895:906	(R)-ketamine	895:906	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	3	6	theme	forced	593:598	arg1	test					609:612	forced swimming test	593:612	forced swimming test	593:612	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	8	7	theme	NFATc4	1466:1471	arg1	pathway					1483:1489	a novel NFATc4 signaling pathway	1458:1489	a novel NFATc4 signaling pathway	1458:1489	In conclusion, our data implicate a novel NFATc4 signaling pathway in the PFC underlying the prophylactic effects of (R)-ketamine for inflammation-related depression.					
35064103	1	8	theme	molecular	211:219	arg1	mechanisms					221:230	the precise molecular mechanisms	199:230	the precise molecular mechanisms underlying its action	199:252	(R, S)-ketamine has prophylactic antidepressant-like effects in rodents; however, the precise molecular mechanisms underlying its action remain unknown.					
35064103	7	9	theme	R	1253:1253	arg1	-ketamine					1255:1263	(R)-ketamine	1252:1263	(R)-ketamine	1252:1263	Similar to (R)-ketamine, gene knockdown of Nfatc4 gene by bilateral injection of adeno-associated virus (AAV) into the mPFC could elicit prophylactic effects in the LPS-treated mice.					
35064103	3	10	theme	lipopolysaccharide	617:634	arg1	mice					661:664	lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice	617:664	lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice	617:664	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	3	11	dep	mg/kg	480:484	arg1	days					489:492	6 days	487:492	6 days before	487:499	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	3	12	theme	test	609:612	arg1	loss					540:543	body weight loss	528:543	body weight loss	528:543	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	3	12	theme	test	609:612	arg1	time					585:588	increased immobility time	564:588	increased immobility time	564:588	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	3	12	theme	test	609:612	arg1	splenomegaly					546:557	splenomegaly	546:557	splenomegaly	546:557	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	6	13	with	pretreatment	1113:1124	arg1	inhibitors					1136:1145	NFAT inhibitors	1131:1145	NFAT inhibitors (i.e., NFAT inhibitor and cyclosporin A)	1131:1186	Furthermore, pretreatment with NFAT inhibitors (i.e., NFAT inhibitor and cyclosporin A) showed prophylactic effects in the LPS-treated mice.					
35064103	1	14	theme	prophylactic	137:148	arg1	effects					170:176	prophylactic antidepressant-like effects	137:176	prophylactic antidepressant-like effects	137:176	(R, S)-ketamine has prophylactic antidepressant-like effects in rodents; however, the precise molecular mechanisms underlying its action remain unknown.					
35064103	4	15	theme	nuclear	784:790	arg1	factor					792:797	the nuclear factor	780:797	the nuclear factor of activated T cells 4 (NFATc4) signaling	780:839	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	4	15	theme	nuclear	784:790	arg1	NFATc4					823:828	NFATc4	823:828	NFATc4	823:828	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	0	16	theme	R	104:104	arg1	-ketamine					106:114	(R)-ketamine	103:114	(R)-ketamine	103:114	Nuclear factor of activated T cells 4 in the prefrontal cortex is required for prophylactic actions of (R)-ketamine.					
35064103	5	17	theme	Quantitative	909:920	arg1	RT-PCR					922:927	Quantitative RT-PCR	909:927	Quantitative RT-PCR	909:927	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	6	18	theme	LPS-treated	1223:1233	arg1	mice					1235:1238	the LPS-treated mice	1219:1238	the LPS-treated mice	1219:1238	Furthermore, pretreatment with NFAT inhibitors (i.e., NFAT inhibitor and cyclosporin A) showed prophylactic effects in the LPS-treated mice.					
35064103	8	19	theme	prophylactic	1517:1528	arg1	effects					1530:1536	the prophylactic effects	1513:1536	the prophylactic effects of (R)-ketamine for inflammation-related depression	1513:1588	In conclusion, our data implicate a novel NFATc4 signaling pathway in the PFC underlying the prophylactic effects of (R)-ketamine for inflammation-related depression.					
35064103	4	20	theme	Pathway	748:754	arg1	Analysis					756:763	Ingenuity Pathway Analysis	738:763	Ingenuity Pathway Analysis	738:763	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	4	20	theme	Pathway	748:754	arg1	IPA					733:735	subsequent IPA	722:735	subsequent IPA (Ingenuity Pathway Analysis)	722:764	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	0	21	theme	-ketamine	106:114	arg1	actions					92:98	prophylactic actions	79:98	prophylactic actions of (R)-ketamine	79:114	Nuclear factor of activated T cells 4 in the prefrontal cortex is required for prophylactic actions of (R)-ketamine.					
35064103	8	22	theme	R	1542:1542	arg1	-ketamine					1544:1552	(R)-ketamine	1541:1552	(R)-ketamine for inflammation-related depression	1541:1588	In conclusion, our data implicate a novel NFATc4 signaling pathway in the PFC underlying the prophylactic effects of (R)-ketamine for inflammation-related depression.					
35064103	5	23	theme	mice	1094:1097	arg1	PFC					1075:1077	the PFC	1071:1077	the PFC of LPS-treated mice	1071:1097	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	8	24	theme	-ketamine	1544:1552	arg1	effects					1530:1536	the prophylactic effects	1513:1536	the prophylactic effects of (R)-ketamine for inflammation-related depression	1513:1588	In conclusion, our data implicate a novel NFATc4 signaling pathway in the PFC underlying the prophylactic effects of (R)-ketamine for inflammation-related depression.					
35064103	2	25	theme	potent	407:412	arg1	enantiomer					414:423	a more potent enantiomer	400:423	a more potent enantiomer of (R, S)-ketamine	400:442	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	2	25	theme	potent	407:412	arg1	-ketamine					389:397	(R)-ketamine	386:397	(R)-ketamine	386:397	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	3	26	dep	lipopolysaccharide	617:634	arg1	LPS					637:639	LPS	637:639	LPS: 1.0 mg/kg	637:650	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	8	27	theme	inflammation-related	1558:1577	arg1	depression					1579:1588	inflammation-related depression	1558:1588	inflammation-related depression	1558:1588	In conclusion, our data implicate a novel NFATc4 signaling pathway in the PFC underlying the prophylactic effects of (R)-ketamine for inflammation-related depression.					
35064103	6	28	theme	NFAT	1154:1157	arg1	inhibitor					1159:1167	NFAT inhibitor	1154:1167	NFAT inhibitor	1154:1167	Furthermore, pretreatment with NFAT inhibitors (i.e., NFAT inhibitor and cyclosporin A) showed prophylactic effects in the LPS-treated mice.					
35064103	3	29	dep	LPS	637:639	arg1	mg/kg					646:650	1.0 mg/kg	642:650	LPS: 1.0 mg/kg	637:650	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	7	30	theme	virus	1339:1343	arg1	injection					1309:1317	bilateral injection	1299:1317	bilateral injection of adeno-associated virus (AAV) into the mPFC	1299:1363	Similar to (R)-ketamine, gene knockdown of Nfatc4 gene by bilateral injection of adeno-associated virus (AAV) into the mPFC could elicit prophylactic effects in the LPS-treated mice.					
35064103	4	31	theme	T	812:812	arg1	cells					814:818	activated T cells 4	802:820	activated T cells 4	802:820	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	0	32	theme	prophylactic	79:90	arg1	actions					92:98	prophylactic actions	79:98	prophylactic actions of (R)-ketamine	79:114	Nuclear factor of activated T cells 4 in the prefrontal cortex is required for prophylactic actions of (R)-ketamine.					
35064103	2	33	theme	R	387:387	arg1	enantiomer					414:423	a more potent enantiomer	400:423	a more potent enantiomer of (R, S)-ketamine	400:442	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	2	33	theme	R	387:387	arg1	-ketamine					389:397	(R)-ketamine	386:397	(R)-ketamine	386:397	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	0	34	theme	Nuclear	0:6	arg1	factor					8:13	Nuclear factor	0:13	Nuclear factor of activated T cells 4 in the prefrontal cortex	0:61	Nuclear factor of activated T cells 4 in the prefrontal cortex is required for prophylactic actions of (R)-ketamine.					
35064103	2	35	theme	prophylactic	362:373	arg1	effects					375:381	the prophylactic effects	358:381	the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine	358:442	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	5	36	theme	signaling	1022:1030	arg1	expression					1001:1010	the increased gene expression	982:1010	the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice	982:1097	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	3	37	theme	body	528:531	arg1	loss					540:543	body weight loss	528:543	body weight loss	528:543	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	0	38	theme	T	28:28	arg1	cells					30:34	activated T cells 4	18:36	activated T cells 4	18:36	Nuclear factor of activated T cells 4 in the prefrontal cortex is required for prophylactic actions of (R)-ketamine.					
35064103	2	39	theme	-ketamine	389:397	arg1	effects					375:381	the prophylactic effects	358:381	the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine	358:442	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	4	40	theme	prefrontal	694:703	arg1	PFC					713:715	PFC	713:715	PFC	713:715	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	4	40	theme	prefrontal	694:703	arg1	cortex					705:710	prefrontal cortex	694:710	prefrontal cortex (PFC)	694:716	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	3	41	with	Pretreatment	445:456	arg1	-ketamine					466:474	(R)-ketamine	463:474	(R)-ketamine (10 mg/kg, 6 days before)	463:500	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	5	42	theme	gene	996:999	arg1	expression					1001:1010	the increased gene expression	982:1010	the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice	982:1097	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	7	43	theme	Nfatc4	1284:1289	arg1	gene					1291:1294	Nfatc4 gene	1284:1294	Nfatc4 gene	1284:1294	Similar to (R)-ketamine, gene knockdown of Nfatc4 gene by bilateral injection of adeno-associated virus (AAV) into the mPFC could elicit prophylactic effects in the LPS-treated mice.					
35064103	4	44	theme	RNA-sequencing	667:680	arg1	analysis					682:689	RNA-sequencing analysis	667:689	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis)	667:764	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	1	45	contain	has	133:135	arg2	effects					170:176	prophylactic antidepressant-like effects	137:176	prophylactic antidepressant-like effects	137:176	(R, S)-ketamine has prophylactic antidepressant-like effects in rodents; however, the precise molecular mechanisms underlying its action remain unknown.					
35064103	1	45	contain	has	133:135	arg1	-ketamine					123:131	(R, S)-ketamine	117:131	(R, S)-ketamine	117:131	(R, S)-ketamine has prophylactic antidepressant-like effects in rodents; however, the precise molecular mechanisms underlying its action remain unknown.					
35064103	2	46	theme	target	329:334	arg1	s					336:336	novel molecular target(s)	313:337	novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine	313:442	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	3	47	theme	immobility	574:583	arg1	time					585:588	increased immobility time	564:588	increased immobility time	564:588	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	2	48	theme	novel	313:317	arg1	s					336:336	novel molecular target(s)	313:337	novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine	313:442	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	1	49	theme	precise	203:209	arg1	mechanisms					221:230	the precise molecular mechanisms	199:230	the precise molecular mechanisms underlying its action	199:252	(R, S)-ketamine has prophylactic antidepressant-like effects in rodents; however, the precise molecular mechanisms underlying its action remain unknown.					
35064103	4	50	theme	IPA	733:735	arg1	analysis					682:689	RNA-sequencing analysis	667:689	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis)	667:764	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	3	51	theme	swimming	600:607	arg1	test					609:612	forced swimming test	593:612	forced swimming test	593:612	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	8	52	theme	novel	1460:1464	arg1	pathway					1483:1489	a novel NFATc4 signaling pathway	1458:1489	a novel NFATc4 signaling pathway	1458:1489	In conclusion, our data implicate a novel NFATc4 signaling pathway in the PFC underlying the prophylactic effects of (R)-ketamine for inflammation-related depression.					
35064103	3	53	dep	-ketamine	466:474	arg1	mg/kg					480:484	10 mg/kg	477:484	10 mg/kg	477:484	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	4	54	theme	cortex	705:710	arg1	analysis					682:689	RNA-sequencing analysis	667:689	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis)	667:764	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	3	55	from	time	585:588	arg1	mice					661:664	lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice	617:664	lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice	617:664	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	3	56	theme	R	464:464	arg1	-ketamine					466:474	(R)-ketamine	463:474	(R)-ketamine (10 mg/kg, 6 days before)	463:500	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	8	57	theme	signaling	1473:1481	arg1	pathway					1483:1489	a novel NFATc4 signaling pathway	1458:1489	a novel NFATc4 signaling pathway	1458:1489	In conclusion, our data implicate a novel NFATc4 signaling pathway in the PFC underlying the prophylactic effects of (R)-ketamine for inflammation-related depression.					
35064103	7	58	theme	LPS-treated	1406:1416	arg1	mice					1418:1421	the LPS-treated mice	1402:1421	the LPS-treated mice	1402:1421	Similar to (R)-ketamine, gene knockdown of Nfatc4 gene by bilateral injection of adeno-associated virus (AAV) into the mPFC could elicit prophylactic effects in the LPS-treated mice.					
35064103	6	59	theme	NFAT	1131:1134	arg1	inhibitors					1136:1145	NFAT inhibitors	1131:1145	NFAT inhibitors (i.e., NFAT inhibitor and cyclosporin A)	1131:1186	Furthermore, pretreatment with NFAT inhibitors (i.e., NFAT inhibitor and cyclosporin A) showed prophylactic effects in the LPS-treated mice.					
35064103	1	60	theme	antidepressant-like	150:168	arg1	effects					170:176	prophylactic antidepressant-like effects	137:176	prophylactic antidepressant-like effects	137:176	(R, S)-ketamine has prophylactic antidepressant-like effects in rodents; however, the precise molecular mechanisms underlying its action remain unknown.					
35064103	7	61	theme	prophylactic	1378:1389	arg1	effects					1391:1397	prophylactic effects	1378:1397	prophylactic effects	1378:1397	Similar to (R)-ketamine, gene knockdown of Nfatc4 gene by bilateral injection of adeno-associated virus (AAV) into the mPFC could elicit prophylactic effects in the LPS-treated mice.					
35064103	4	62	theme	Ingenuity	738:746	arg1	Analysis					756:763	Ingenuity Pathway Analysis	738:763	Ingenuity Pathway Analysis	738:763	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	4	62	theme	Ingenuity	738:746	arg1	IPA					733:735	subsequent IPA	722:735	subsequent IPA (Ingenuity Pathway Analysis)	722:764	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	5	63	theme	LPS-treated	1082:1092	arg1	mice					1094:1097	LPS-treated mice	1082:1097	LPS-treated mice	1082:1097	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	0	64	theme	prefrontal	45:54	arg1	cortex					56:61	the prefrontal cortex	41:61	the prefrontal cortex	41:61	Nuclear factor of activated T cells 4 in the prefrontal cortex is required for prophylactic actions of (R)-ketamine.					
35064103	4	65	theme	cells	814:818	arg1	factor					792:797	the nuclear factor	780:797	the nuclear factor of activated T cells 4 (NFATc4) signaling	780:839	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	4	65	theme	cells	814:818	arg1	NFATc4					823:828	NFATc4	823:828	NFATc4	823:828	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	5	66	dep	signaling	1022:1030	arg1	Cd4					1041:1043	Cd4	1041:1043	Cd4	1041:1043	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	5	66	dep	signaling	1022:1030	arg1	Nfatc4					1033:1038	Nfatc4	1033:1038	Nfatc4	1033:1038	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	5	66	dep	signaling	1022:1030	arg1	H2-ab1					1053:1058	H2-ab1	1053:1058	H2-ab1	1053:1058	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	5	66	dep	signaling	1022:1030	arg1	H2-aa					1061:1065	H2-aa	1061:1065	H2-aa	1061:1065	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	5	66	dep	signaling	1022:1030	arg1	Cd79b					1046:1050	Cd79b	1046:1050	Cd79b	1046:1050	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	3	67	theme	-treated	652:659	arg1	mice					661:664	lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice	617:664	lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice	617:664	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	6	68	theme	cyclosporin	1173:1183	arg1	A					1185:1185	cyclosporin A	1173:1185	cyclosporin A	1173:1185	Furthermore, pretreatment with NFAT inhibitors (i.e., NFAT inhibitor and cyclosporin A) showed prophylactic effects in the LPS-treated mice.					
35064103	0	69	from	factor	8:13	arg1	cortex					56:61	the prefrontal cortex	41:61	the prefrontal cortex	41:61	Nuclear factor of activated T cells 4 in the prefrontal cortex is required for prophylactic actions of (R)-ketamine.					
35064103	6	70	dep	inhibitor	1159:1167	arg1	i.e.					1148:1151	i.e.	1148:1151	i.e.	1148:1151	Furthermore, pretreatment with NFAT inhibitors (i.e., NFAT inhibitor and cyclosporin A) showed prophylactic effects in the LPS-treated mice.					
35064103	7	71	theme	adeno-associated	1322:1337	arg1	AAV					1346:1348	AAV	1346:1348	AAV	1346:1348	Similar to (R)-ketamine, gene knockdown of Nfatc4 gene by bilateral injection of adeno-associated virus (AAV) into the mPFC could elicit prophylactic effects in the LPS-treated mice.					
35064103	7	71	theme	adeno-associated	1322:1337	arg1	virus					1339:1343	adeno-associated virus	1322:1343	adeno-associated virus (AAV)	1322:1349	Similar to (R)-ketamine, gene knockdown of Nfatc4 gene by bilateral injection of adeno-associated virus (AAV) into the mPFC could elicit prophylactic effects in the LPS-treated mice.					
35064103	4	72	theme	factor	792:797	arg1	signaling					831:839	the nuclear factor of activated T cells 4 (NFATc4) signaling	780:839	the nuclear factor of activated T cells 4 (NFATc4) signaling	780:839	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	4	73	theme	activated	802:810	arg1	cells					814:818	activated T cells 4	802:820	activated T cells 4	802:820	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	5	74	theme	R	945:945	arg1	-ketamine					947:955	(R)-ketamine	944:955	(R)-ketamine	944:955	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	2	75	theme	-ketamine	434:442	arg1	enantiomer					414:423	a more potent enantiomer	400:423	a more potent enantiomer of (R, S)-ketamine	400:442	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	2	75	theme	-ketamine	434:442	arg1	-ketamine					389:397	(R)-ketamine	386:397	(R)-ketamine	386:397	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	0	76	theme	activated	18:26	arg1	cells					30:34	activated T cells 4	18:36	activated T cells 4	18:36	Nuclear factor of activated T cells 4 in the prefrontal cortex is required for prophylactic actions of (R)-ketamine.					
35064103	5	77	theme	NFATc4	1015:1020	arg1	signaling					1022:1030	NFATc4 signaling	1015:1030	NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa)	1015:1066	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	4	78	theme	prophylactic	871:882	arg1	effects					884:890	sustained prophylactic effects	861:890	sustained prophylactic effects of (R)-ketamine	861:906	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	0	79	theme	cells	30:34	arg1	factor					8:13	Nuclear factor	0:13	Nuclear factor of activated T cells 4 in the prefrontal cortex	0:61	Nuclear factor of activated T cells 4 in the prefrontal cortex is required for prophylactic actions of (R)-ketamine.					
35064103	1	80	dep	-ketamine	123:131	arg1	S					121:121	S	121:121	S	121:121	(R, S)-ketamine has prophylactic antidepressant-like effects in rodents; however, the precise molecular mechanisms underlying its action remain unknown.					
35064103	1	80	dep	-ketamine	123:131	arg1	R					118:118	R	118:118	R	118:118	(R, S)-ketamine has prophylactic antidepressant-like effects in rodents; however, the precise molecular mechanisms underlying its action remain unknown.					
35064103	6	81	theme	prophylactic	1195:1206	arg1	effects					1208:1214	prophylactic effects	1195:1214	prophylactic effects	1195:1214	Furthermore, pretreatment with NFAT inhibitors (i.e., NFAT inhibitor and cyclosporin A) showed prophylactic effects in the LPS-treated mice.					
35064103	5	82	theme	increased	986:994	arg1	expression					1001:1010	the increased gene expression	982:1010	the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice	982:1097	Quantitative RT-PCR confirmed that (R)-ketamine significantly attenuated the increased gene expression of NFATc4 signaling (Nfatc4, Cd4, Cd79b, H2-ab1, H2-aa) in the PFC of LPS-treated mice.					
35064103	7	83	theme	bilateral	1299:1307	arg1	injection					1309:1317	bilateral injection	1299:1317	bilateral injection of adeno-associated virus (AAV) into the mPFC	1299:1363	Similar to (R)-ketamine, gene knockdown of Nfatc4 gene by bilateral injection of adeno-associated virus (AAV) into the mPFC could elicit prophylactic effects in the LPS-treated mice.					
35064103	2	84	theme	molecular	319:327	arg1	s					336:336	novel molecular target(s)	313:337	novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine	313:442	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	7	85	theme	gene	1291:1294	arg1	knockdown					1271:1279	gene knockdown	1266:1279	gene knockdown of Nfatc4 gene by bilateral injection of adeno-associated virus (AAV) into the mPFC	1266:1363	Similar to (R)-ketamine, gene knockdown of Nfatc4 gene by bilateral injection of adeno-associated virus (AAV) into the mPFC could elicit prophylactic effects in the LPS-treated mice.					
35064103	4	86	theme	-ketamine	898:906	arg1	effects					884:890	sustained prophylactic effects	861:890	sustained prophylactic effects of (R)-ketamine	861:906	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35064103	3	87	theme	weight	533:538	arg1	loss					540:543	body weight loss	528:543	body weight loss	528:543	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	2	88	theme	RNA-sequencing	276:289	arg1	analysis					291:298	RNA-sequencing analysis	276:298	RNA-sequencing analysis	276:298	Using RNA-sequencing analysis, we searched novel molecular target(s) that contribute to the prophylactic effects of (R)-ketamine, a more potent enantiomer of (R, S)-ketamine.					
35064103	3	89	from	loss	540:543	arg1	mice					661:664	lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice	617:664	lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice	617:664	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	3	90	theme	increased	564:572	arg1	time					585:588	increased immobility time	564:588	increased immobility time	564:588	Pretreatment with (R)-ketamine (10 mg/kg, 6 days before) significantly ameliorated body weight loss, splenomegaly, and increased immobility time of forced swimming test in lipopolysaccharide (LPS: 1.0 mg/kg)-treated mice.					
35064103	4	91	theme	sustained	861:869	arg1	effects					884:890	sustained prophylactic effects	861:890	sustained prophylactic effects of (R)-ketamine	861:906	RNA-sequencing analysis of prefrontal cortex (PFC) and subsequent IPA (Ingenuity Pathway Analysis) revealed that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of (R)-ketamine.					
35840057	16	0	contain	have	3002:3005	arg2	capacity					3015:3022	a great capacity	3007:3022	a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential	3007:3147	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	16	0	contain	have	3002:3005	arg1	oils					2957:2960	the essential oils	2943:2960	the essential oils of P. gaudichaudianum and P. mikanianum	2943:3000	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	15	1	theme	antichemotactic	2861:2875	arg1	mechanism					2850:2858	this mechanism	2845:2858	this mechanism (antichemotactic assay) for essential oils this species	2845:2914	CONCLUSIONS Since up to date there is no report of this biological activities by this mechanism (antichemotactic assay) for essential oils this species.					
35840057	15	1	theme	antichemotactic	2861:2875	arg1	assay					2877:2881	antichemotactic assay	2861:2881	antichemotactic assay	2861:2881	CONCLUSIONS Since up to date there is no report of this biological activities by this mechanism (antichemotactic assay) for essential oils this species.					
35840057	4	2	theme	neutrophil	949:958	arg1	activity					976:983	neutrophil antichemotactic activity	949:983	neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases	949:1203	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	1	3	theme	damaged	265:271	arg1	cells					273:277	damaged cells	265:277	damaged cells	265:277	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation is the body's normal protective response to injury and is stimulated by pathogens, toxic compounds, damaged cells or radiation, promoting healing and restoring homeostasis to the injured tissue.					
35840057	4	4	theme	STUDY	796:800	arg1	AIM					785:787	AIM	785:787	AIM OF THE STUDY	785:800	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	4	5	theme	activity	976:983	arg1	oil					869:871	the essential oil	855:871	the essential oil of Piper gaudichaudianum and Piper mikanianum	855:917	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	4	5	theme	activity	976:983	arg1	evaluation					935:944	the evaluation	931:944	the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases	931:1203	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	12	6	theme	mikanianum	2227:2236	arg1	oil					2248:2250	P. mikanianum essential oil	2224:2250	P. mikanianum essential oil	2224:2250	For P. mikanianum essential oil, β-myrcene (17.2%) and bicyclogermacrene (26.3%) were the major components in the monoterpenes and sesquiterpene fractions, respectively.					
35840057	3	7	theme	inflammation	656:667	arg1	treatment					643:651	the treatment	639:651	the treatment of inflammation, rheumatism and ulcer	639:689	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	9	8	dep	in	1701:1702	arg1	vitro					1704:1708	vitro	1704:1708	vitro	1704:1708	The in vitro anti-inflammatory activity was evaluated using the model of modified Boyden chamber.					
35840057	4	9	dep	the	1078:1080	arg1	leaves					1082:1087	leaves	1082:1087	leaves	1082:1087	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	9	10	theme	chamber	1786:1792	arg1	model					1761:1765	the model	1757:1765	the model of modified Boyden chamber	1757:1792	The in vitro anti-inflammatory activity was evaluated using the model of modified Boyden chamber.					
35840057	12	11	theme	major	2310:2314	arg1	components					2316:2325	the major components	2306:2325	the major components in the monoterpenes and sesquiterpene fractions	2306:2373	For P. mikanianum essential oil, β-myrcene (17.2%) and bicyclogermacrene (26.3%) were the major components in the monoterpenes and sesquiterpene fractions, respectively.					
35840057	12	11	theme	major	2310:2314	arg1	bicyclogermacrene					2275:2291	bicyclogermacrene	2275:2291	bicyclogermacrene	2275:2291	For P. mikanianum essential oil, β-myrcene (17.2%) and bicyclogermacrene (26.3%) were the major components in the monoterpenes and sesquiterpene fractions, respectively.					
35840057	12	11	theme	major	2310:2314	arg1	β-myrcene					2253:2261	β-myrcene	2253:2261	β-myrcene	2253:2261	For P. mikanianum essential oil, β-myrcene (17.2%) and bicyclogermacrene (26.3%) were the major components in the monoterpenes and sesquiterpene fractions, respectively.					
35840057	9	12	theme	modified	1770:1777	arg1	chamber					1786:1792	modified Boyden chamber	1770:1792	modified Boyden chamber	1770:1792	The in vitro anti-inflammatory activity was evaluated using the model of modified Boyden chamber.					
35840057	11	13	theme	caryophyllene	2167:2179	arg1	oxide					2181:2185	caryophyllene oxide	2167:2185	caryophyllene oxide (9.3%)	2167:2192	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	11	13	theme	caryophyllene	2167:2179	arg1	%					2191:2191	9.3%	2188:2191	9.3%	2188:2191	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	3	14	theme	ulcer	685:689	arg1	treatment					643:651	the treatment	639:651	the treatment of inflammation, rheumatism and ulcer	639:689	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	5	15	theme	fresh	1307:1311	arg1	samples					1313:1319	crushed fresh samples	1299:1319	crushed fresh samples	1299:1319	MATERIALS AND METHODS The essential oil from leaves of both Piper species were obtained from crushed fresh samples, by hydrodistillation using a Clevenger type-apparatus for 4 h.					
35840057	11	16	theme	essential	1980:1988	arg1	oils					1990:1993	the essential oils	1976:1993	the essential oils	1976:1993	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	17	17	from	use	3289:3291	arg1	treatment					3317:3325	the treatment	3313:3325	the treatment of inflammatory processes	3313:3351	Findings of these studies support the traditional use of these species in the treatment of inflammatory processes.					
35840057	3	18	theme	rheumatism	670:679	arg1	treatment					643:651	the treatment	639:651	the treatment of inflammation, rheumatism and ulcer	639:689	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	14	19	theme	%	2665:2665	arg1	migration					2610:2618	migration	2610:2618	migration of 0-72.2% for P. gaudichaudianum and 8.6-100% for P. mikanianum to same concentrations	2610:2706	Both essential oils showed antichemotactic effect with reduction in migration of 0-72.2% for P. gaudichaudianum and 8.6-100% for P. mikanianum to same concentrations, suggesting a response to acute inflammatory processes.					
35840057	14	20	theme	inflammatory	2740:2751	arg1	processes					2753:2761	acute inflammatory processes	2734:2761	acute inflammatory processes	2734:2761	Both essential oils showed antichemotactic effect with reduction in migration of 0-72.2% for P. gaudichaudianum and 8.6-100% for P. mikanianum to same concentrations, suggesting a response to acute inflammatory processes.					
35840057	8	21	theme	chromatography-flame	1645:1664	arg1	GC-FID					1688:1693	GC-FID	1688:1693	GC-FID	1688:1693	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35840057	8	21	theme	chromatography-flame	1645:1664	arg1	detection					1677:1685	gas chromatography-flame ionization detection	1641:1685	gas chromatography-flame ionization detection (GC-FID)	1641:1694	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35840057	16	22	theme	injury	3188:3193	arg1	site					3195:3198	the injury site	3184:3198	the injury site	3184:3198	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	1	23	theme	RELEVANCE	142:150	arg1	Inflammation					152:163	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation	121:163	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation	121:163	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation is the body's normal protective response to injury and is stimulated by pathogens, toxic compounds, damaged cells or radiation, promoting healing and restoring homeostasis to the injured tissue.					
35840057	10	24	dep	Escherichia	1903:1913	arg1	coli					1915:1918	coli	1915:1918	coli	1915:1918	In this test the essential oils were tested for their ability to inhibit leukocyte chemotaxis stimulated by Escherichia coli lipopolysaccharide.					
35840057	4	25	theme	plant	1092:1096	arg1	material					1098:1105	plant material	1092:1105	plant material	1092:1105	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	3	26	theme	stomach	722:728	arg1	disorders					730:738	stomach disorders	722:738	stomach disorders	722:738	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	4	27	dep	complement	1020:1029	arg1	taking					1071:1076	taking	1071:1076	taking the leaves as plant material	1071:1105	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	7	28	theme	essential	1472:1480	arg1	oil					1482:1484	essential oil	1472:1484	essential oil obtained	1472:1493	The amount of essential oil obtained was quantified in mL.					
35840057	4	29	theme	gaudichaudianum	882:896	arg1	oil					869:871	the essential oil	855:871	the essential oil of Piper gaudichaudianum and Piper mikanianum	855:917	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	4	29	theme	gaudichaudianum	882:896	arg1	evaluation					935:944	the evaluation	931:944	the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases	931:1203	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	12	30	theme	sesquiterpene	2351:2363	arg1	fractions					2365:2373	the monoterpenes and sesquiterpene fractions	2330:2373	fractions	2365:2373	For P. mikanianum essential oil, β-myrcene (17.2%) and bicyclogermacrene (26.3%) were the major components in the monoterpenes and sesquiterpene fractions, respectively.					
35840057	14	31	theme	%	2629:2629	arg1	migration					2610:2618	migration	2610:2618	migration of 0-72.2% for P. gaudichaudianum and 8.6-100% for P. mikanianum to same concentrations	2610:2706	Both essential oils showed antichemotactic effect with reduction in migration of 0-72.2% for P. gaudichaudianum and 8.6-100% for P. mikanianum to same concentrations, suggesting a response to acute inflammatory processes.					
35840057	4	32	theme	use	1066:1068	arg1	information					1035:1045	the information	1031:1045	the information of its traditional use	1031:1068	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	0	33	theme	oils	69:72	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition and anti-inflammatory activity of the essential oils of Piper gaudichaudianum and Piper mikanianum.					
35840057	0	33	theme	oils	69:72	arg1	activity					43:50	anti-inflammatory activity	25:50	anti-inflammatory activity	25:50	Chemical composition and anti-inflammatory activity of the essential oils of Piper gaudichaudianum and Piper mikanianum.					
35840057	2	34	theme	toothache	472:480	arg1	relief					462:467	the relief	458:467	the relief of toothache	458:480	Leaves of Piper gaudichaudianum Kunth, known as "pariparoba" are widely used in folk medicine for the relief of toothache, while the fresh roots are used as anti-inflammatory and to treat liver disorders.					
35840057	3	35	used	used	713:716	arg2	diaphoretic					749:759	a diaphoretic	747:759	a diaphoretic in intermittent fevers	747:782	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	3	35	used	used	713:716	arg2	roots					701:705	its roots	697:705	its roots	697:705	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	11	36	dep	β-selinene	2090:2099	arg1	compounds					2144:2152	the main compounds	2135:2152	the main compounds	2135:2152	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	8	37	dep	identification	1521:1534	arg1	The					1517:1519	The	1517:1519	The	1517:1519	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35840057	2	38	used	used	509:512	arg2	roots					499:503	the fresh roots	489:503	the fresh roots	489:503	Leaves of Piper gaudichaudianum Kunth, known as "pariparoba" are widely used in folk medicine for the relief of toothache, while the fresh roots are used as anti-inflammatory and to treat liver disorders.					
35840057	2	38	used	used	509:512	arg2	anti-inflammatory					517:533	anti-inflammatory	517:533	anti-inflammatory	517:533	Leaves of Piper gaudichaudianum Kunth, known as "pariparoba" are widely used in folk medicine for the relief of toothache, while the fresh roots are used as anti-inflammatory and to treat liver disorders.					
35840057	0	39	theme	Piper	77:81	arg1	gaudichaudianum					83:97	Piper gaudichaudianum	77:97	Piper gaudichaudianum	77:97	Chemical composition and anti-inflammatory activity of the essential oils of Piper gaudichaudianum and Piper mikanianum.					
35840057	1	40	theme	normal	179:184	arg1	response					197:204	the body's normal protective response	168:204	the body's normal protective response to injury	168:214	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation is the body's normal protective response to injury and is stimulated by pathogens, toxic compounds, damaged cells or radiation, promoting healing and restoring homeostasis to the injured tissue.					
35840057	4	41	theme	essential	859:867	arg1	oil					869:871	the essential oil	855:871	the essential oil of Piper gaudichaudianum and Piper mikanianum	855:917	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	16	42	theme	dose-dependent	3090:3103	arg1	way					3105:3107	a dose-dependent way	3088:3107	a dose-dependent way	3088:3107	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	15	43	dep	oils	2898:2901	arg1	species					2908:2914	this species	2903:2914	essential oils this species	2888:2914	CONCLUSIONS Since up to date there is no report of this biological activities by this mechanism (antichemotactic assay) for essential oils this species.					
35840057	2	44	theme	fresh	493:497	arg1	anti-inflammatory					517:533	anti-inflammatory	517:533	anti-inflammatory	517:533	Leaves of Piper gaudichaudianum Kunth, known as "pariparoba" are widely used in folk medicine for the relief of toothache, while the fresh roots are used as anti-inflammatory and to treat liver disorders.					
35840057	2	44	theme	fresh	493:497	arg1	roots					499:503	the fresh roots	489:503	the fresh roots	489:503	Leaves of Piper gaudichaudianum Kunth, known as "pariparoba" are widely used in folk medicine for the relief of toothache, while the fresh roots are used as anti-inflammatory and to treat liver disorders.					
35840057	8	45	theme	gas	1592:1594	arg1	GC-MS					1630:1634	GC-MS	1630:1634	GC-MS	1630:1634	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35840057	8	45	theme	gas	1592:1594	arg1	spectrometry					1616:1627	gas chromatography-mass spectrometry	1592:1627	gas chromatography-mass spectrometry (GC-MS)	1592:1635	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35840057	14	46	from	reduction	2597:2605	arg1	migration					2610:2618	migration	2610:2618	migration of 0-72.2% for P. gaudichaudianum and 8.6-100% for P. mikanianum to same concentrations	2610:2706	Both essential oils showed antichemotactic effect with reduction in migration of 0-72.2% for P. gaudichaudianum and 8.6-100% for P. mikanianum to same concentrations, suggesting a response to acute inflammatory processes.					
35840057	0	47	theme	mikanianum	109:118	arg1	oils					69:72	the essential oils	55:72	the essential oils of Piper gaudichaudianum and Piper mikanianum	55:118	Chemical composition and anti-inflammatory activity of the essential oils of Piper gaudichaudianum and Piper mikanianum.					
35840057	2	48	theme	folk	440:443	arg1	medicine					445:452	folk medicine	440:452	folk medicine	440:452	Leaves of Piper gaudichaudianum Kunth, known as "pariparoba" are widely used in folk medicine for the relief of toothache, while the fresh roots are used as anti-inflammatory and to treat liver disorders.					
35840057	4	49	theme	diseases	1196:1203	arg1	treatment					1170:1178	the treatment	1166:1178	the treatment of inflammatory diseases	1166:1203	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	0	50	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition and anti-inflammatory activity of the essential oils of Piper gaudichaudianum and Piper mikanianum.					
35840057	5	51	from	leaves	1251:1256	arg1	MATERIALS					1206:1214	MATERIALS	1206:1214	MATERIALS	1206:1214	MATERIALS AND METHODS The essential oil from leaves of both Piper species were obtained from crushed fresh samples, by hydrodistillation using a Clevenger type-apparatus for 4 h.					
35840057	5	51	from	leaves	1251:1256	arg1	METHODS					1220:1226	METHODS	1220:1226	METHODS	1220:1226	MATERIALS AND METHODS The essential oil from leaves of both Piper species were obtained from crushed fresh samples, by hydrodistillation using a Clevenger type-apparatus for 4 h.					
35840057	15	52	theme	activities	2831:2840	arg1	report					2805:2810	no report	2802:2810	no report of this biological activities by this mechanism (antichemotactic assay) for essential oils this species	2802:2914	CONCLUSIONS Since up to date there is no report of this biological activities by this mechanism (antichemotactic assay) for essential oils this species.					
35840057	17	53	theme	inflammatory	3330:3341	arg1	processes					3343:3351	inflammatory processes	3330:3351	inflammatory processes	3330:3351	Findings of these studies support the traditional use of these species in the treatment of inflammatory processes.					
35840057	2	54	theme	pariparoba	409:418	arg1	"					419:419	"pariparoba"	408:419	"pariparoba"	408:419	Leaves of Piper gaudichaudianum Kunth, known as "pariparoba" are widely used in folk medicine for the relief of toothache, while the fresh roots are used as anti-inflammatory and to treat liver disorders.					
35840057	17	55	theme	traditional	3277:3287	arg1	use					3289:3291	the traditional use	3273:3291	the traditional use of these species in the treatment of inflammatory processes	3273:3351	Findings of these studies support the traditional use of these species in the treatment of inflammatory processes.					
35840057	17	56	theme	processes	3343:3351	arg1	treatment					3317:3325	the treatment	3313:3325	the treatment of inflammatory processes	3313:3351	Findings of these studies support the traditional use of these species in the treatment of inflammatory processes.					
35840057	14	57	theme	essential	2547:2555	arg1	oils					2557:2560	Both essential oils	2542:2560	Both essential oils	2542:2560	Both essential oils showed antichemotactic effect with reduction in migration of 0-72.2% for P. gaudichaudianum and 8.6-100% for P. mikanianum to same concentrations, suggesting a response to acute inflammatory processes.					
35840057	4	58	theme	essential	993:1001	arg1	oils					1003:1006	both essential oils	988:1006	both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases	988:1203	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	5	59	dep	MATERIALS	1206:1214	arg1	oil					1242:1244	The essential oil	1228:1244	The essential oil	1228:1244	MATERIALS AND METHODS The essential oil from leaves of both Piper species were obtained from crushed fresh samples, by hydrodistillation using a Clevenger type-apparatus for 4 h.					
35840057	12	60	theme	essential	2238:2246	arg1	oil					2248:2250	P. mikanianum essential oil	2224:2250	P. mikanianum essential oil	2224:2250	For P. mikanianum essential oil, β-myrcene (17.2%) and bicyclogermacrene (26.3%) were the major components in the monoterpenes and sesquiterpene fractions, respectively.					
35840057	17	61	theme	species	3302:3308	arg1	use					3289:3291	the traditional use	3273:3291	the traditional use of these species in the treatment of inflammatory processes	3273:3351	Findings of these studies support the traditional use of these species in the treatment of inflammatory processes.					
35840057	2	62	theme	gaudichaudianum	376:390	arg1	Kunth					392:396	Piper gaudichaudianum Kunth	370:396	Piper gaudichaudianum Kunth	370:396	Leaves of Piper gaudichaudianum Kunth, known as "pariparoba" are widely used in folk medicine for the relief of toothache, while the fresh roots are used as anti-inflammatory and to treat liver disorders.					
35840057	16	63	theme	gaudichaudianum	2968:2982	arg1	oils					2957:2960	the essential oils	2943:2960	the essential oils of P. gaudichaudianum and P. mikanianum	2943:3000	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	1	64	theme	toxic	248:252	arg1	compounds					254:262	toxic compounds	248:262	toxic compounds	248:262	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation is the body's normal protective response to injury and is stimulated by pathogens, toxic compounds, damaged cells or radiation, promoting healing and restoring homeostasis to the injured tissue.					
35840057	9	65	theme	Boyden	1779:1784	arg1	chamber					1786:1792	modified Boyden chamber	1770:1792	modified Boyden chamber	1770:1792	The in vitro anti-inflammatory activity was evaluated using the model of modified Boyden chamber.					
35840057	4	66	theme	Piper	902:906	arg1	mikanianum					908:917	Piper mikanianum	902:917	Piper mikanianum	902:917	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	13	67	dep	Escherichia	2505:2515	arg1	coli					2517:2520	coli	2517:2520	coli	2517:2520	The essential oils were also tested for their ability to inhibit neutrophil chemotaxis in vitro when stimulated by Escherichia coli lipopolysaccharide.					
35840057	11	68	theme	constituents	2029:2040	arg1	identification					2008:2021	the identification	2004:2021	the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds	2004:2152	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	12	69	theme	P.	2224:2225	arg1	oil					2248:2250	P. mikanianum essential oil	2224:2250	P. mikanianum essential oil	2224:2250	For P. mikanianum essential oil, β-myrcene (17.2%) and bicyclogermacrene (26.3%) were the major components in the monoterpenes and sesquiterpene fractions, respectively.					
35840057	2	70	used	used	432:435	arg2	Leaves					360:365	Leaves	360:365	Leaves	360:365	Leaves of Piper gaudichaudianum Kunth, known as "pariparoba" are widely used in folk medicine for the relief of toothache, while the fresh roots are used as anti-inflammatory and to treat liver disorders.					
35840057	1	71	theme	protective	186:195	arg1	response					197:204	the body's normal protective response	168:204	the body's normal protective response to injury	168:214	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation is the body's normal protective response to injury and is stimulated by pathogens, toxic compounds, damaged cells or radiation, promoting healing and restoring homeostasis to the injured tissue.					
35840057	11	72	theme	sesquiterpenes	2075:2088	arg1	β-selinene					2090:2099	the sesquiterpenes β-selinene	2071:2099	the sesquiterpenes β-selinene (14.0%)	2071:2107	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	11	72	theme	sesquiterpenes	2075:2088	arg1	%					2106:2106	14.0%	2102:2106	14.0%	2102:2106	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	14	73	theme	same	2688:2691	arg1	concentrations					2693:2706	same concentrations	2688:2706	same concentrations	2688:2706	Both essential oils showed antichemotactic effect with reduction in migration of 0-72.2% for P. gaudichaudianum and 8.6-100% for P. mikanianum to same concentrations, suggesting a response to acute inflammatory processes.					
35840057	4	74	theme	traditional	1054:1064	arg1	use					1066:1068	its traditional use	1050:1068	its traditional use	1050:1068	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	5	75	theme	crushed	1299:1305	arg1	samples					1313:1319	crushed fresh samples	1299:1319	crushed fresh samples	1299:1319	MATERIALS AND METHODS The essential oil from leaves of both Piper species were obtained from crushed fresh samples, by hydrodistillation using a Clevenger type-apparatus for 4 h.					
35840057	11	76	theme	chemical	1952:1959	arg1	composition					1961:1971	The chemical composition	1948:1971	RESULTS The chemical composition of the essential oils	1940:1993	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	8	77	theme	chromatography-mass	1596:1614	arg1	GC-MS					1630:1634	GC-MS	1630:1634	GC-MS	1630:1634	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35840057	8	77	theme	chromatography-mass	1596:1614	arg1	spectrometry					1616:1627	gas chromatography-mass spectrometry	1592:1627	gas chromatography-mass spectrometry (GC-MS)	1592:1635	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35840057	17	78	theme	studies	3257:3263	arg1	Findings					3239:3246	Findings	3239:3246	Findings of these studies	3239:3263	Findings of these studies support the traditional use of these species in the treatment of inflammatory processes.					
35840057	13	79	theme	Escherichia	2505:2515	arg1	lipopolysaccharide					2522:2539	Escherichia coli lipopolysaccharide	2505:2539	Escherichia coli lipopolysaccharide	2505:2539	The essential oils were also tested for their ability to inhibit neutrophil chemotaxis in vitro when stimulated by Escherichia coli lipopolysaccharide.					
35840057	11	80	theme	oils	1990:1993	arg1	composition					1961:1971	The chemical composition	1948:1971	RESULTS The chemical composition of the essential oils	1940:1993	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	16	81	theme	essential	2947:2955	arg1	oils					2957:2960	the essential oils	2943:2960	the essential oils of P. gaudichaudianum and P. mikanianum	2943:3000	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	12	82	theme	monoterpenes	2334:2345	arg1	fractions					2365:2373	the monoterpenes and sesquiterpene fractions	2330:2373	fractions	2365:2373	For P. mikanianum essential oil, β-myrcene (17.2%) and bicyclogermacrene (26.3%) were the major components in the monoterpenes and sesquiterpene fractions, respectively.					
35840057	1	83	dep	pathogens	237:245	arg1	promoting					293:301	promoting	293:301	promoting healing	293:309	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation is the body's normal protective response to injury and is stimulated by pathogens, toxic compounds, damaged cells or radiation, promoting healing and restoring homeostasis to the injured tissue.					
35840057	1	83	dep	pathogens	237:245	arg1	restoring					315:323	restoring	315:323	restoring homeostasis to the injured tissue	315:357	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation is the body's normal protective response to injury and is stimulated by pathogens, toxic compounds, damaged cells or radiation, promoting healing and restoring homeostasis to the injured tissue.					
35840057	14	84	theme	acute	2734:2738	arg1	processes					2753:2761	acute inflammatory processes	2734:2761	acute inflammatory processes	2734:2761	Both essential oils showed antichemotactic effect with reduction in migration of 0-72.2% for P. gaudichaudianum and 8.6-100% for P. mikanianum to same concentrations, suggesting a response to acute inflammatory processes.					
35840057	8	85	theme	gas	1641:1643	arg1	GC-FID					1688:1693	GC-FID	1688:1693	GC-FID	1688:1693	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35840057	8	85	theme	gas	1641:1643	arg1	detection					1677:1685	gas chromatography-flame ionization detection	1641:1685	gas chromatography-flame ionization detection (GC-FID)	1641:1694	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35840057	1	86	theme	ETHNOPHARMACOLOGICAL	121:140	arg1	Inflammation					152:163	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation	121:163	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation	121:163	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation is the body's normal protective response to injury and is stimulated by pathogens, toxic compounds, damaged cells or radiation, promoting healing and restoring homeostasis to the injured tissue.					
35840057	7	87	theme	oil	1482:1484	arg1	amount					1462:1467	The amount	1458:1467	The amount of essential oil obtained	1458:1493	The amount of essential oil obtained was quantified in mL.					
35840057	7	87	theme	oil	1482:1484	arg1	oil					1482:1484	essential oil	1472:1484	essential oil obtained	1472:1493	The amount of essential oil obtained was quantified in mL.					
35840057	2	88	theme	liver	548:552	arg1	disorders					554:562	liver disorders	548:562	liver disorders	548:562	Leaves of Piper gaudichaudianum Kunth, known as "pariparoba" are widely used in folk medicine for the relief of toothache, while the fresh roots are used as anti-inflammatory and to treat liver disorders.					
35840057	16	89	theme	inflammatory	3063:3074	arg1	process					3076:3082	an inflammatory process	3060:3082	an inflammatory process	3060:3082	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	10	90	theme	leukocyte	1868:1876	arg1	chemotaxis					1878:1887	leukocyte chemotaxis	1868:1887	leukocyte chemotaxis stimulated by Escherichia coli lipopolysaccharide	1868:1937	In this test the essential oils were tested for their ability to inhibit leukocyte chemotaxis stimulated by Escherichia coli lipopolysaccharide.					
35840057	8	91	theme	ionization	1666:1675	arg1	GC-FID					1688:1693	GC-FID	1688:1693	GC-FID	1688:1693	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35840057	8	91	theme	ionization	1666:1675	arg1	detection					1677:1685	gas chromatography-flame ionization detection	1641:1685	gas chromatography-flame ionization detection (GC-FID)	1641:1694	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35840057	11	92	dep	RESULTS	1940:1946	arg1	composition					1961:1971	The chemical composition	1948:1971	RESULTS The chemical composition of the essential oils	1940:1993	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	3	93	theme	intermittent	764:775	arg1	fevers					777:782	intermittent fevers	764:782	intermittent fevers	764:782	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	4	94	theme	Piper	876:880	arg1	gaudichaudianum					882:896	Piper gaudichaudianum	876:896	Piper gaudichaudianum	876:896	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	16	95	theme	anti-inflammatory	3121:3137	arg1	potential					3139:3147	anti-inflammatory potential	3121:3147	anti-inflammatory potential	3121:3147	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	16	96	theme	damage	3231:3236	arg1	possibility					3209:3219	the possibility	3205:3219	the possibility of tissue damage	3205:3236	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	10	97	theme	Escherichia	1903:1913	arg1	lipopolysaccharide					1920:1937	Escherichia coli lipopolysaccharide	1903:1937	Escherichia coli lipopolysaccharide	1903:1937	In this test the essential oils were tested for their ability to inhibit leukocyte chemotaxis stimulated by Escherichia coli lipopolysaccharide.					
35840057	0	98	theme	gaudichaudianum	83:97	arg1	oils					69:72	the essential oils	55:72	the essential oils of Piper gaudichaudianum and Piper mikanianum	55:118	Chemical composition and anti-inflammatory activity of the essential oils of Piper gaudichaudianum and Piper mikanianum.					
35840057	1	99	theme	injured	344:350	arg1	tissue					352:357	the injured tissue	340:357	the injured tissue	340:357	ETHNOPHARMACOLOGICAL RELEVANCE Inflammation is the body's normal protective response to injury and is stimulated by pathogens, toxic compounds, damaged cells or radiation, promoting healing and restoring homeostasis to the injured tissue.					
35840057	16	100	theme	mikanianum	2991:3000	arg1	oils					2957:2960	the essential oils	2943:2960	the essential oils of P. gaudichaudianum and P. mikanianum	2943:3000	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	0	101	theme	Piper	103:107	arg1	mikanianum					109:118	Piper mikanianum	103:118	Piper mikanianum	103:118	Chemical composition and anti-inflammatory activity of the essential oils of Piper gaudichaudianum and Piper mikanianum.					
35840057	16	102	theme	great	3009:3013	arg1	capacity					3015:3022	a great capacity	3007:3022	a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential	3007:3147	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	4	103	from	use	1141:1143	arg1	medicine					1153:1160	folk medicine	1148:1160	folk medicine	1148:1160	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	13	104	theme	neutrophil	2455:2464	arg1	chemotaxis					2466:2475	neutrophil chemotaxis	2455:2475	neutrophil chemotaxis	2455:2475	The essential oils were also tested for their ability to inhibit neutrophil chemotaxis in vitro when stimulated by Escherichia coli lipopolysaccharide.					
35840057	12	105	from	components	2316:2325	arg1	fractions					2365:2373	the monoterpenes and sesquiterpene fractions	2330:2373	fractions	2365:2373	For P. mikanianum essential oil, β-myrcene (17.2%) and bicyclogermacrene (26.3%) were the major components in the monoterpenes and sesquiterpene fractions, respectively.					
35840057	5	106	theme	Clevenger	1351:1359	arg1	type-apparatus					1361:1374	a Clevenger type-apparatus	1349:1374	a Clevenger type-apparatus for 4 h	1349:1382	MATERIALS AND METHODS The essential oil from leaves of both Piper species were obtained from crushed fresh samples, by hydrodistillation using a Clevenger type-apparatus for 4 h.					
35840057	16	107	theme	tissue	3224:3229	arg1	damage					3231:3236	tissue damage	3224:3236	tissue damage	3224:3236	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	5	108	theme	essential	1232:1240	arg1	oil					1242:1244	The essential oil	1228:1244	The essential oil	1228:1244	MATERIALS AND METHODS The essential oil from leaves of both Piper species were obtained from crushed fresh samples, by hydrodistillation using a Clevenger type-apparatus for 4 h.					
35840057	11	109	theme	main	2139:2142	arg1	compounds					2144:2152	the main compounds	2135:2152	the main compounds	2135:2152	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	16	110	theme	neutrophil	3035:3044	arg1	chemotaxis					3046:3055	neutrophil chemotaxis	3035:3055	neutrophil chemotaxis	3035:3055	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	9	111	theme	in	1701:1702	arg1	activity					1728:1735	The in vitro anti-inflammatory activity	1697:1735	The in vitro anti-inflammatory activity	1697:1735	The in vitro anti-inflammatory activity was evaluated using the model of modified Boyden chamber.					
35840057	0	112	theme	anti-inflammatory	25:41	arg1	activity					43:50	anti-inflammatory activity	25:50	anti-inflammatory activity	25:50	Chemical composition and anti-inflammatory activity of the essential oils of Piper gaudichaudianum and Piper mikanianum.					
35840057	15	113	theme	biological	2820:2829	arg1	activities					2831:2840	this biological activities	2815:2840	this biological activities	2815:2840	CONCLUSIONS Since up to date there is no report of this biological activities by this mechanism (antichemotactic assay) for essential oils this species.					
35840057	9	114	theme	anti-inflammatory	1710:1726	arg1	activity					1728:1735	The in vitro anti-inflammatory activity	1697:1735	The in vitro anti-inflammatory activity	1697:1735	The in vitro anti-inflammatory activity was evaluated using the model of modified Boyden chamber.					
35840057	13	115	theme	essential	2394:2402	arg1	oils					2404:2407	The essential oils	2390:2407	The essential oils	2390:2407	The essential oils were also tested for their ability to inhibit neutrophil chemotaxis in vitro when stimulated by Escherichia coli lipopolysaccharide.					
35840057	4	116	theme	oils	1003:1006	arg1	activity					976:983	neutrophil antichemotactic activity	949:983	neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases	949:1203	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	14	117	theme	antichemotactic	2569:2583	arg1	effect					2585:2590	antichemotactic effect	2569:2590	antichemotactic effect	2569:2590	Both essential oils showed antichemotactic effect with reduction in migration of 0-72.2% for P. gaudichaudianum and 8.6-100% for P. mikanianum to same concentrations, suggesting a response to acute inflammatory processes.					
35840057	4	118	theme	mikanianum	908:917	arg1	oil					869:871	the essential oil	855:871	the essential oil of Piper gaudichaudianum and Piper mikanianum	855:917	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	4	118	theme	mikanianum	908:917	arg1	evaluation					935:944	the evaluation	931:944	the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases	931:1203	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	3	119	from	diaphoretic	749:759	arg1	fevers					777:782	intermittent fevers	764:782	intermittent fevers	764:782	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	4	120	theme	inflammatory	1183:1194	arg1	diseases					1196:1203	inflammatory diseases	1183:1203	inflammatory diseases	1183:1203	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	5	121	theme	Piper	1266:1270	arg1	species					1272:1278	both Piper species	1261:1278	both Piper species	1261:1278	MATERIALS AND METHODS The essential oil from leaves of both Piper species were obtained from crushed fresh samples, by hydrodistillation using a Clevenger type-apparatus for 4 h.					
35840057	0	122	theme	essential	59:67	arg1	oils					69:72	the essential oils	55:72	the essential oils of Piper gaudichaudianum and Piper mikanianum	55:118	Chemical composition and anti-inflammatory activity of the essential oils of Piper gaudichaudianum and Piper mikanianum.					
35840057	4	123	theme	folk	1148:1151	arg1	medicine					1153:1160	folk medicine	1148:1160	folk medicine	1148:1160	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	6	124	theme	yield	1389:1393	arg1	determination					1395:1407	The yield determination	1385:1407	The yield determination	1385:1407	The yield determination was performed as vol/wt (v/w) and in triplicate.					
35840057	6	124	theme	yield	1389:1393	arg1	vol/wt					1426:1431	vol/wt	1426:1431	vol/wt (v/w)	1426:1437	The yield determination was performed as vol/wt (v/w) and in triplicate.					
35840057	15	125	theme	essential	2888:2896	arg1	oils					2898:2901	essential oils this species	2888:2914	essential oils this species	2888:2914	CONCLUSIONS Since up to date there is no report of this biological activities by this mechanism (antichemotactic assay) for essential oils this species.					
35840057	3	126	used	used	631:634	arg2	Steud					591:595	Steud	591:595	Steud	591:595	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	3	126	used	used	631:634	arg2	"					618:618	"aguaxima"	609:618	"aguaxima"	609:618	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	3	127	theme	aguaxima	610:617	arg1	"					618:618	"aguaxima"	609:618	"aguaxima"	609:618	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	3	127	theme	aguaxima	610:617	arg1	Steud					591:595	Steud	591:595	Steud	591:595	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	3	128	dep	known	600:604	arg1	used					631:634	used	631:634	used	631:634	For P. mikanianum (Kunth) Steud is known as "aguaxima", is widely used in the treatment of inflammation, rheumatism and ulcer, with its roots being used for stomach disorders and as a diaphoretic in intermittent fevers.					
35840057	16	129	from	site	3195:3198	arg1	accumulation					3168:3179	its accumulation	3164:3179	its accumulation at the injury site	3164:3198	These results showed that the essential oils of P. gaudichaudianum and P. mikanianum have a great capacity to inhibit neutrophil chemotaxis in an inflammatory process, in a dose-dependent way, suggesting anti-inflammatory potential, by preventing its accumulation at the injury site with the possibility of tissue damage.					
35840057	4	130	theme	antichemotactic	960:974	arg1	activity					976:983	neutrophil antichemotactic activity	949:983	neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases	949:1203	AIM OF THE STUDY Therefore, this work aims to chemically characterize the essential oil of Piper gaudichaudianum and Piper mikanianum, as well as the evaluation of neutrophil antichemotactic activity of both essential oils in order to complement the information of its traditional use, taking the leaves as plant material and, with that, corroborating its use in folk medicine for the treatment of inflammatory diseases.					
35840057	11	131	theme	E	2199:2199	arg1	-nerolidol					2201:2210	(E)-nerolidol	2198:2210	(E)-nerolidol (9.0%)	2198:2217	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	11	131	theme	E	2199:2199	arg1	%					2216:2216	9.0%	2213:2216	9.0%	2213:2216	RESULTS The chemical composition of the essential oils revealed the identification of 26 constituents for P. gaudichaudianum being the sesquiterpenes β-selinene (14.0%) and viridiflorene (10.5%) the main compounds, followed by caryophyllene oxide (9.3%) and (E)-nerolidol (9.0%).					
35840057	10	132	theme	essential	1812:1820	arg1	oils					1822:1825	the essential oils	1808:1825	the essential oils	1808:1825	In this test the essential oils were tested for their ability to inhibit leukocyte chemotaxis stimulated by Escherichia coli lipopolysaccharide.					
35840057	2	133	theme	Piper	370:374	arg1	Kunth					392:396	Piper gaudichaudianum Kunth	370:396	Piper gaudichaudianum Kunth	370:396	Leaves of Piper gaudichaudianum Kunth, known as "pariparoba" are widely used in folk medicine for the relief of toothache, while the fresh roots are used as anti-inflammatory and to treat liver disorders.					
35840057	8	134	theme	compounds	1562:1570	arg1	quantification					1540:1553	quantification	1540:1553	quantification	1540:1553	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35840057	8	134	theme	compounds	1562:1570	arg1	identification					1521:1534	identification	1521:1534	identification	1521:1534	The identification and quantification of the compounds was performed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).					
35491853	12	0	theme	soil	2136:2139	arg1	matter					2149:2154	soil organic matter	2136:2154	soil organic matter formation	2136:2164	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	17	1	from	enriched	2959:2966	arg1	NPK					2976:2978	NPK	2976:2978	NPK	2976:2978	Our study demonstrated that Dothideomycetes dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more enriched in both NPK and unfertilized soils.					
35491853	17	1	from	enriched	2959:2966	arg1	soils					2997:3001	unfertilized soils	2984:3001	unfertilized soils	2984:3001	Our study demonstrated that Dothideomycetes dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more enriched in both NPK and unfertilized soils.					
35491853	14	2	theme	species	2322:2328	arg1	responses					2302:2310	responses	2302:2310	responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process	2302:2424	However, current understanding of responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process is still very superficial.					
35491853	3	3	theme	nitrogen-phosphorus-potassium	502:530	arg1	fertilizer					538:547	nitrogen-phosphorus-potassium (NPK) fertilizer	502:547	nitrogen-phosphorus-potassium (NPK) fertilizer	502:547	Here, we applied [12C]cellulose and [13C]cellulose to distinguish the effects of application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control) for 27 years upon cellulose decomposition via RNA-based stable isotope probing (RNA-SIP).					
35491853	4	4	from	ratio	677:681	arg1	soil					715:718	compost soil	707:718	compost soil	707:718	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	1	5	theme	matter	275:280	arg1	formation					282:290	soil organic matter formation	262:290	soil organic matter formation	262:290	Cellulose is the most abundant polysaccharide in plant biomass and an important precursor of soil organic matter formation.					
35491853	16	6	with	technique	2669:2677	arg1	sequencing					2711:2720	subsequent high-throughput sequencing	2684:2720	subsequent high-throughput sequencing	2684:2720	The novel 13C-RNA-SIP technique with subsequent high-throughput sequencing was used to investigate the linkages between active fungal taxa and cellulose decomposition.					
35491853	5	7	from	NPK	979:981	arg1	Sordariomycetes					941:955	the copiotrophic Sordariomycetes	924:955	the copiotrophic Sordariomycetes	924:955	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	5	7	from	NPK	979:981	arg1	abundant					967:974	abundant	967:974	abundant	967:974	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	18	8	theme	cellulose-rich	3157:3170	arg1	substrates					3172:3181	cellulose-rich substrates	3157:3181	cellulose-rich substrates	3157:3181	We also found that the compost amendment promoted fungal diversity and phylogenetic relatedness and strengthened the decomposition capacity of fungi for cellulose-rich substrates by enhancing synergistic interactions, thereby reducing cellulose C sequestration efficiency.					
35491853	11	9	theme	active	1990:1995	arg1	fungi					1997:2001	active fungi	1990:2001	active fungi	1990:2001	This work also highlights the RNA-SIP technique's value for comprehensively assessing the contributions of active fungi to the substrate decomposition process.					
35491853	3	10	theme	fertilizer	538:547	arg1	application					478:488	application	478:488	application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control)	478:576	Here, we applied [12C]cellulose and [13C]cellulose to distinguish the effects of application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control) for 27 years upon cellulose decomposition via RNA-based stable isotope probing (RNA-SIP).					
35491853	5	11	from	abundant	967:974	arg1	NPK					979:981	NPK	979:981	NPK	979:981	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	5	11	from	abundant	967:974	arg1	soils					1000:1004	unfertilized soils	987:1004	unfertilized soils	987:1004	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	1	12	theme	abundant	191:198	arg1	polysaccharide					200:213	the most abundant polysaccharide	182:213	the most abundant polysaccharide in plant biomass	182:230	Cellulose is the most abundant polysaccharide in plant biomass and an important precursor of soil organic matter formation.					
35491853	1	12	theme	abundant	191:198	arg1	Cellulose					169:177	Cellulose	169:177	Cellulose	169:177	Cellulose is the most abundant polysaccharide in plant biomass and an important precursor of soil organic matter formation.					
35491853	12	13	theme	rich	2074:2077	arg1	IMPORTANCE					2043:2052	IMPORTANCE	2043:2052	IMPORTANCE Cellulose	2043:2062	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	12	13	theme	rich	2074:2077	arg1	component					2079:2087	a very rich component	2067:2087	a very rich component in plant biomass	2067:2104	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	17	14	theme	compost	2894:2900	arg1	soil					2902:2905	compost soil	2894:2905	compost soil	2894:2905	Our study demonstrated that Dothideomycetes dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more enriched in both NPK and unfertilized soils.					
35491853	16	15	theme	fungal	2774:2779	arg1	taxa					2781:2784	active fungal taxa	2767:2784	active fungal taxa	2767:2784	The novel 13C-RNA-SIP technique with subsequent high-throughput sequencing was used to investigate the linkages between active fungal taxa and cellulose decomposition.					
35491853	14	16	theme	current	2277:2283	arg1	understanding					2285:2297	current understanding	2277:2297	current understanding of responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process	2277:2424	However, current understanding of responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process is still very superficial.					
35491853	2	17	theme	carbon	318:323	arg1	dynamics					333:340	carbon cycling dynamics	318:340	carbon cycling dynamics	318:340	Fungi play a key role in carbon cycling dynamics because they tend to decompose recalcitrant materials.					
35491853	15	18	theme	cellulose	2481:2489	arg1	experiment					2501:2510	a [13C]cellulose microcosm experiment	2474:2510	a [13C]cellulose microcosm experiment with soils subjected to long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control)	2474:2644	Here, we established a [13C]cellulose microcosm experiment with soils subjected to long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control).					
35491853	3	19	theme	fertilizer	557:566	arg1	application					478:488	application	478:488	application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control)	478:576	Here, we applied [12C]cellulose and [13C]cellulose to distinguish the effects of application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control) for 27 years upon cellulose decomposition via RNA-based stable isotope probing (RNA-SIP).					
35491853	19	20	contain	has	3299:3301	arg2	implications					3303:3314	implications	3303:3314	implications for our understanding of the role of active fungi in cellulose C transformation	3303:3394	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	19	20	contain	has	3299:3301	arg1	research					3290:3297	our research	3286:3297	our research	3286:3297	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	19	21	theme	cellulose	3369:3377	arg1	transformation					3381:3394	cellulose C transformation	3369:3394	cellulose C transformation	3369:3394	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	11	22	theme	RNA-SIP	1913:1919	arg1	technique					1921:1929	the RNA-SIP technique's	1909:1931	the RNA-SIP technique's value for comprehensively assessing the contributions of active fungi to the substrate decomposition process	1909:2040	This work also highlights the RNA-SIP technique's value for comprehensively assessing the contributions of active fungi to the substrate decomposition process.					
35491853	0	23	theme	Phylogenetic	143:154	arg1	Relatedness					156:166	Phylogenetic Relatedness	143:166	Phylogenetic Relatedness	143:166	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.					
35491853	13	24	theme	carbon	2231:2236	arg1	accumulation					2238:2249	organic carbon accumulation	2223:2249	organic carbon accumulation	2223:2249	Fungal communities are known to be important drivers of organic carbon accumulation in arable soils.					
35491853	7	25	theme	compost	1153:1159	arg1	soil					1161:1164	compost soil	1153:1164	compost soil	1153:1164	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	15	26	theme	fertilizer	2606:2615	arg1	application					2546:2556	long-term application	2536:2556	long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control)	2536:2644	Here, we established a [13C]cellulose microcosm experiment with soils subjected to long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control).					
35491853	8	27	theme	species	1481:1487	arg1	capacity					1462:1469	an augmented decomposition capacity	1435:1469	an augmented decomposition capacity of fungal species for cellulose-rich substrates	1435:1517	These changes led to an augmented decomposition capacity of fungal species for cellulose-rich substrates and reduced cellulose C sequestration efficiency.					
35491853	4	28	theme	C	702:702	arg1	higher					739:744	higher	739:744	higher	739:744	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	4	28	theme	C	702:702	arg1	ratio					677:681	The loss ratio	668:681	The loss ratio of added cellulose C in compost soil	668:718	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	18	29	theme	decomposition	3121:3133	arg1	capacity					3135:3142	the decomposition capacity	3117:3142	the decomposition capacity of fungi for cellulose-rich substrates	3117:3181	We also found that the compost amendment promoted fungal diversity and phylogenetic relatedness and strengthened the decomposition capacity of fungi for cellulose-rich substrates by enhancing synergistic interactions, thereby reducing cellulose C sequestration efficiency.					
35491853	15	30	theme	NPK	2601:2603	arg1	fertilizer					2606:2615	nitrogen-phosphorus-potassium (NPK) fertilizer	2570:2615	nitrogen-phosphorus-potassium (NPK) fertilizer	2570:2615	Here, we established a [13C]cellulose microcosm experiment with soils subjected to long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control).					
35491853	19	31	from	role	3345:3348	arg1	transformation					3381:3394	cellulose C transformation	3369:3394	cellulose C transformation	3369:3394	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	16	32	theme	active	2767:2772	arg1	taxa					2781:2784	active fungal taxa	2767:2784	active fungal taxa	2767:2784	The novel 13C-RNA-SIP technique with subsequent high-throughput sequencing was used to investigate the linkages between active fungal taxa and cellulose decomposition.					
35491853	8	33	theme	cellulose-rich	1493:1506	arg1	substrates					1508:1517	cellulose-rich substrates	1493:1517	cellulose-rich substrates	1493:1517	These changes led to an augmented decomposition capacity of fungal species for cellulose-rich substrates and reduced cellulose C sequestration efficiency.					
35491853	7	34	theme	invasion	1373:1380	arg1	filtering					1311:1319	stringent filtering	1301:1319	stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost	1301:1411	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	4	35	theme	compost	707:713	arg1	soil					715:718	compost soil	707:718	compost soil	707:718	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	19	36	theme	C	3379:3379	arg1	transformation					3381:3394	cellulose C transformation	3369:3394	cellulose C transformation	3369:3394	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	1	37	from	precursor	249:257	arg1	biomass					224:230	plant biomass	218:230	plant biomass	218:230	Cellulose is the most abundant polysaccharide in plant biomass and an important precursor of soil organic matter formation.					
35491853	13	38	theme	important	2202:2210	arg1	drivers					2212:2218	important drivers	2202:2218	important drivers of organic carbon accumulation in arable soils	2202:2265	Fungal communities are known to be important drivers of organic carbon accumulation in arable soils.					
35491853	3	39	theme	RNA-based	624:632	arg1	isotope					641:647	RNA-based stable isotope	624:647	RNA-based stable isotope probing (RNA-SIP)	624:665	Here, we applied [12C]cellulose and [13C]cellulose to distinguish the effects of application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control) for 27 years upon cellulose decomposition via RNA-based stable isotope probing (RNA-SIP).					
35491853	1	40	from	polysaccharide	200:213	arg1	biomass					224:230	plant biomass	218:230	plant biomass	218:230	Cellulose is the most abundant polysaccharide in plant biomass and an important precursor of soil organic matter formation.					
35491853	5	41	theme	compost	902:908	arg1	soil					910:913	compost soil	902:913	compost soil	902:913	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	16	42	theme	novel	2651:2655	arg1	technique					2669:2677	The novel 13C-RNA-SIP technique	2647:2677	The novel 13C-RNA-SIP technique with subsequent high-throughput sequencing	2647:2720	The novel 13C-RNA-SIP technique with subsequent high-throughput sequencing was used to investigate the linkages between active fungal taxa and cellulose decomposition.					
35491853	12	43	theme	matter	2149:2154	arg1	formation					2156:2164	soil organic matter formation	2136:2164	soil organic matter formation	2136:2164	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	13	44	theme	Fungal	2167:2172	arg1	communities					2174:2184	Fungal communities	2167:2184	Fungal communities	2167:2184	Fungal communities are known to be important drivers of organic carbon accumulation in arable soils.					
35491853	15	45	theme	compost	2561:2567	arg1	application					2546:2556	long-term application	2536:2556	long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control)	2536:2644	Here, we established a [13C]cellulose microcosm experiment with soils subjected to long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control).					
35491853	5	46	theme	Cryptococcus	853:864	arg1	members					832:838	members	832:838	mainly members of the genus Cryptococcus	825:864	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	8	47	theme	cellulose	1531:1539	arg1	efficiency					1557:1566	cellulose C sequestration efficiency	1531:1566	cellulose C sequestration efficiency	1531:1566	These changes led to an augmented decomposition capacity of fungal species for cellulose-rich substrates and reduced cellulose C sequestration efficiency.					
35491853	4	48	theme	added	686:690	arg1	C					702:702	added cellulose C	686:702	added cellulose C in compost soil	686:718	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	0	49	theme	Fungal	79:84	arg1	Composition					96:106	Fungal Community Composition	79:106	Fungal Community Composition	79:106	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.					
35491853	4	50	dep	106.7	732:736	arg1	to					729:730	to	729:730	to	729:730	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	16	51	theme	subsequent	2684:2693	arg1	sequencing					2711:2720	subsequent high-throughput sequencing	2684:2720	subsequent high-throughput sequencing	2684:2720	The novel 13C-RNA-SIP technique with subsequent high-throughput sequencing was used to investigate the linkages between active fungal taxa and cellulose decomposition.					
35491853	17	52	theme	unfertilized	2984:2995	arg1	soils					2997:3001	unfertilized soils	2984:3001	unfertilized soils	2984:3001	Our study demonstrated that Dothideomycetes dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more enriched in both NPK and unfertilized soils.					
35491853	8	53	theme	sequestration	1543:1555	arg1	efficiency					1557:1566	cellulose C sequestration efficiency	1531:1566	cellulose C sequestration efficiency	1531:1566	These changes led to an augmented decomposition capacity of fungal species for cellulose-rich substrates and reduced cellulose C sequestration efficiency.					
35491853	16	54	used	used	2726:2729	arg2	technique					2669:2677	The novel 13C-RNA-SIP technique	2647:2677	The novel 13C-RNA-SIP technique with subsequent high-throughput sequencing	2647:2720	The novel 13C-RNA-SIP technique with subsequent high-throughput sequencing was used to investigate the linkages between active fungal taxa and cellulose decomposition.					
35491853	6	55	dep	NPK	1021:1023	arg1	soils					1037:1041	soils	1037:1041	soils	1037:1041	Compared with NPK and control soils, compost application increased the diversity of 13C-assimilating fungi.					
35491853	14	56	theme	decomposition	2404:2416	arg1	process					2418:2424	substrate decomposition process	2394:2424	substrate decomposition process	2394:2424	However, current understanding of responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process is still very superficial.					
35491853	15	57	theme	nitrogen-phosphorus-potassium	2570:2598	arg1	fertilizer					2606:2615	nitrogen-phosphorus-potassium (NPK) fertilizer	2570:2615	nitrogen-phosphorus-potassium (NPK) fertilizer	2570:2615	Here, we established a [13C]cellulose microcosm experiment with soils subjected to long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control).					
35491853	18	58	theme	fungal	3054:3059	arg1	diversity					3061:3069	fungal diversity	3054:3069	fungal diversity	3054:3069	We also found that the compost amendment promoted fungal diversity and phylogenetic relatedness and strengthened the decomposition capacity of fungi for cellulose-rich substrates by enhancing synergistic interactions, thereby reducing cellulose C sequestration efficiency.					
35491853	19	59	theme	long-term	3435:3443	arg1	management					3454:3463	long-term nutrient management	3435:3463	long-term nutrient management	3435:3463	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	15	60	theme	fertilizer	2625:2634	arg1	application					2546:2556	long-term application	2536:2556	long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control)	2536:2644	Here, we established a [13C]cellulose microcosm experiment with soils subjected to long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control).					
35491853	7	61	theme	resources	1348:1356	arg1	filtering					1311:1319	stringent filtering	1301:1319	stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost	1301:1411	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	18	62	theme	synergistic	3196:3206	arg1	interactions					3208:3219	synergistic interactions	3196:3219	synergistic interactions	3196:3219	We also found that the compost amendment promoted fungal diversity and phylogenetic relatedness and strengthened the decomposition capacity of fungi for cellulose-rich substrates by enhancing synergistic interactions, thereby reducing cellulose C sequestration efficiency.					
35491853	14	63	theme	active	2378:2383	arg1	fungi					2385:2389	active fungi	2378:2389	active fungi	2378:2389	However, current understanding of responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process is still very superficial.					
35491853	10	64	theme	long-term	1692:1700	arg1	application					1710:1720	long-term compost application	1692:1720	long-term compost application	1692:1720	Overall, long-term compost application modified fungal community composition and promoted fungal diversity and phylogenetic relatedness, accelerating the decomposition of substrate cellulose in soil.					
35491853	0	65	theme	Long-Term	0:8	arg1	Decomposition					44:56	Long-Term Compost Amendment Spurs Cellulose Decomposition	0:56	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.	0:167	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.					
35491853	7	66	theme	narrow-spectrum	1324:1338	arg1	resources					1348:1356	narrow-spectrum organic resources	1324:1356	narrow-spectrum organic resources	1324:1356	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	5	67	theme	unfertilized	987:998	arg1	soils					1000:1004	unfertilized soils	987:1004	unfertilized soils	987:1004	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	0	68	theme	Amendment	18:26	arg1	Decomposition					44:56	Long-Term Compost Amendment Spurs Cellulose Decomposition	0:56	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.	0:167	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.					
35491853	11	69	theme	substrate	2010:2018	arg1	process					2034:2040	the substrate decomposition process	2006:2040	the substrate decomposition process	2006:2040	This work also highlights the RNA-SIP technique's value for comprehensively assessing the contributions of active fungi to the substrate decomposition process.					
35491853	19	70	theme	management	3454:3463	arg1	types					3426:3430	different types	3416:3430	different types of long-term nutrient management	3416:3463	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	10	71	theme	fungal	1731:1736	arg1	composition					1748:1758	fungal community composition	1731:1758	fungal community composition	1731:1758	Overall, long-term compost application modified fungal community composition and promoted fungal diversity and phylogenetic relatedness, accelerating the decomposition of substrate cellulose in soil.					
35491853	0	72	theme	Cellulose	34:42	arg1	Decomposition					44:56	Long-Term Compost Amendment Spurs Cellulose Decomposition	0:56	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.	0:167	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.					
35491853	18	73	theme	sequestration	3251:3263	arg1	efficiency					3265:3274	cellulose C sequestration efficiency	3239:3274	cellulose C sequestration efficiency	3239:3274	We also found that the compost amendment promoted fungal diversity and phylogenetic relatedness and strengthened the decomposition capacity of fungi for cellulose-rich substrates by enhancing synergistic interactions, thereby reducing cellulose C sequestration efficiency.					
35491853	9	74	theme	soil	1642:1645	arg1	availability					1656:1667	altered soil resource availability	1634:1667	altered soil resource availability	1634:1667	The RNA-SIP technique is more sensitive to responses of fungi to altered soil resource availability than DNA-SIP.					
35491853	3	75	theme	isotope	641:647	arg1	probing					649:655	RNA-based stable isotope probing	624:655	RNA-based stable isotope probing (RNA-SIP)	624:665	Here, we applied [12C]cellulose and [13C]cellulose to distinguish the effects of application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control) for 27 years upon cellulose decomposition via RNA-based stable isotope probing (RNA-SIP).					
35491853	3	75	theme	isotope	641:647	arg1	RNA-SIP					658:664	RNA-SIP	658:664	RNA-SIP	658:664	Here, we applied [12C]cellulose and [13C]cellulose to distinguish the effects of application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control) for 27 years upon cellulose decomposition via RNA-based stable isotope probing (RNA-SIP).					
35491853	4	76	theme	20-day	789:794	arg1	incubation					796:805	their 20-day incubation	783:805	their 20-day incubation	783:805	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	7	77	theme	fungal	1131:1136	arg1	communities					1138:1148	The 13C-labeled fungal communities	1115:1148	The 13C-labeled fungal communities in compost soil	1115:1164	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	18	78	theme	cellulose	3239:3247	arg1	efficiency					3265:3274	cellulose C sequestration efficiency	3239:3274	cellulose C sequestration efficiency	3239:3274	We also found that the compost amendment promoted fungal diversity and phylogenetic relatedness and strengthened the decomposition capacity of fungi for cellulose-rich substrates by enhancing synergistic interactions, thereby reducing cellulose C sequestration efficiency.					
35491853	17	79	from	NPK	2976:2978	arg1	Sordariomycetes					2933:2947	the copiotrophic Sordariomycetes	2916:2947	the copiotrophic Sordariomycetes	2916:2947	Our study demonstrated that Dothideomycetes dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more enriched in both NPK and unfertilized soils.					
35491853	17	79	from	NPK	2976:2978	arg1	enriched					2959:2966	enriched	2959:2966	enriched	2959:2966	Our study demonstrated that Dothideomycetes dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more enriched in both NPK and unfertilized soils.					
35491853	8	80	theme	augmented	1438:1446	arg1	capacity					1462:1469	an augmented decomposition capacity	1435:1469	an augmented decomposition capacity of fungal species for cellulose-rich substrates	1435:1517	These changes led to an augmented decomposition capacity of fungal species for cellulose-rich substrates and reduced cellulose C sequestration efficiency.					
35491853	1	81	theme	important	239:247	arg1	precursor					249:257	an important precursor	236:257	an important precursor of soil organic matter formation	236:290	Cellulose is the most abundant polysaccharide in plant biomass and an important precursor of soil organic matter formation.					
35491853	6	82	theme	13C-assimilating	1091:1106	arg1	fungi					1108:1112	13C-assimilating fungi	1091:1112	13C-assimilating fungi	1091:1112	Compared with NPK and control soils, compost application increased the diversity of 13C-assimilating fungi.					
35491853	4	83	theme	control	762:768	arg1	soils					770:774	control soils	762:774	control soils	762:774	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	12	84	theme	important	2113:2121	arg1	precursor					2123:2131	an important precursor	2110:2131	an important precursor of soil organic matter formation	2110:2164	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	7	85	theme	greater	1217:1223	arg1	relatedness					1233:1243	greater species relatedness	1217:1243	greater species relatedness	1217:1243	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	10	86	theme	phylogenetic	1794:1805	arg1	relatedness					1807:1817	phylogenetic relatedness	1794:1817	phylogenetic relatedness	1794:1817	Overall, long-term compost application modified fungal community composition and promoted fungal diversity and phylogenetic relatedness, accelerating the decomposition of substrate cellulose in soil.					
35491853	1	87	theme	organic	267:273	arg1	matter					275:280	soil organic matter	262:280	soil organic matter formation	262:290	Cellulose is the most abundant polysaccharide in plant biomass and an important precursor of soil organic matter formation.					
35491853	12	88	from	precursor	2123:2131	arg1	biomass					2098:2104	plant biomass	2092:2104	plant biomass	2092:2104	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	17	89	from	soils	2997:3001	arg1	Sordariomycetes					2933:2947	the copiotrophic Sordariomycetes	2916:2947	the copiotrophic Sordariomycetes	2916:2947	Our study demonstrated that Dothideomycetes dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more enriched in both NPK and unfertilized soils.					
35491853	17	89	from	soils	2997:3001	arg1	enriched					2959:2966	enriched	2959:2966	enriched	2959:2966	Our study demonstrated that Dothideomycetes dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more enriched in both NPK and unfertilized soils.					
35491853	14	90	theme	fungal	2315:2320	arg1	species					2322:2328	fungal species	2315:2328	fungal species	2315:2328	However, current understanding of responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process is still very superficial.					
35491853	2	91	theme	key	306:308	arg1	role					310:313	a key role	304:313	a key role	304:313	Fungi play a key role in carbon cycling dynamics because they tend to decompose recalcitrant materials.					
35491853	1	92	theme	formation	282:290	arg1	polysaccharide					200:213	the most abundant polysaccharide	182:213	the most abundant polysaccharide in plant biomass	182:230	Cellulose is the most abundant polysaccharide in plant biomass and an important precursor of soil organic matter formation.					
35491853	1	92	theme	formation	282:290	arg1	Cellulose					169:177	Cellulose	169:177	Cellulose	169:177	Cellulose is the most abundant polysaccharide in plant biomass and an important precursor of soil organic matter formation.					
35491853	1	92	theme	formation	282:290	arg1	precursor					249:257	an important precursor	236:257	an important precursor of soil organic matter formation	236:290	Cellulose is the most abundant polysaccharide in plant biomass and an important precursor of soil organic matter formation.					
35491853	12	93	theme	plant	2092:2096	arg1	biomass					2098:2104	plant biomass	2092:2104	plant biomass	2092:2104	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	17	94	theme	cellulose	2869:2877	arg1	utilization					2879:2889	cellulose utilization	2869:2889	cellulose utilization	2869:2889	Our study demonstrated that Dothideomycetes dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more enriched in both NPK and unfertilized soils.					
35491853	19	95	from	understanding	3324:3336	arg1	transformation					3381:3394	cellulose C transformation	3369:3394	cellulose C transformation	3369:3394	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	14	96	theme	responses	2302:2310	arg1	understanding					2285:2297	current understanding	2277:2297	current understanding of responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process	2277:2424	However, current understanding of responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process is still very superficial.					
35491853	0	97	from	Shifts	69:74	arg1	Composition					96:106	Fungal Community Composition	79:106	Fungal Community Composition	79:106	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.					
35491853	7	98	theme	species	1225:1231	arg1	relatedness					1233:1243	greater species relatedness	1217:1243	greater species relatedness	1217:1243	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	19	99	theme	active	3353:3358	arg1	fungi					3360:3364	active fungi	3353:3364	active fungi	3353:3364	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	2	100	theme	cycling	325:331	arg1	dynamics					333:340	carbon cycling dynamics	318:340	carbon cycling dynamics	318:340	Fungi play a key role in carbon cycling dynamics because they tend to decompose recalcitrant materials.					
35491853	19	101	theme	role	3345:3348	arg1	understanding					3324:3336	our understanding	3320:3336	our understanding of the role of active fungi in cellulose C transformation	3320:3394	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	10	102	theme	cellulose	1864:1872	arg1	decomposition					1837:1849	the decomposition	1833:1849	the decomposition of substrate cellulose in soil	1833:1880	Overall, long-term compost application modified fungal community composition and promoted fungal diversity and phylogenetic relatedness, accelerating the decomposition of substrate cellulose in soil.					
35491853	1	103	theme	plant	218:222	arg1	biomass					224:230	plant biomass	218:230	plant biomass	218:230	Cellulose is the most abundant polysaccharide in plant biomass and an important precursor of soil organic matter formation.					
35491853	8	104	theme	fungal	1474:1479	arg1	species					1481:1487	fungal species	1474:1487	fungal species	1474:1487	These changes led to an augmented decomposition capacity of fungal species for cellulose-rich substrates and reduced cellulose C sequestration efficiency.					
35491853	10	105	theme	fungal	1773:1778	arg1	diversity					1780:1788	fungal diversity	1773:1788	fungal diversity	1773:1788	Overall, long-term compost application modified fungal community composition and promoted fungal diversity and phylogenetic relatedness, accelerating the decomposition of substrate cellulose in soil.					
35491853	13	106	theme	organic	2223:2229	arg1	accumulation					2238:2249	organic carbon accumulation	2223:2249	organic carbon accumulation	2223:2249	Fungal communities are known to be important drivers of organic carbon accumulation in arable soils.					
35491853	5	107	from	soils	1000:1004	arg1	Sordariomycetes					941:955	the copiotrophic Sordariomycetes	924:955	the copiotrophic Sordariomycetes	924:955	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	5	107	from	soils	1000:1004	arg1	abundant					967:974	abundant	967:974	abundant	967:974	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	13	108	theme	accumulation	2238:2249	arg1	drivers					2212:2218	important drivers	2202:2218	important drivers of organic carbon accumulation in arable soils	2202:2265	Fungal communities are known to be important drivers of organic carbon accumulation in arable soils.					
35491853	5	109	theme	cellulose	877:885	arg1	utilization					887:897	cellulose utilization	877:897	cellulose utilization	877:897	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	13	110	theme	arable	2254:2259	arg1	soils					2261:2265	arable soils	2254:2265	arable soils	2254:2265	Fungal communities are known to be important drivers of organic carbon accumulation in arable soils.					
35491853	7	111	theme	added	1399:1403	arg1	compost					1405:1411	added compost	1399:1411	added compost	1399:1411	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	4	112	theme	cellulose	692:700	arg1	C					702:702	added cellulose C	686:702	added cellulose C in compost soil	686:718	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	15	113	with	experiment	2501:2510	arg1	soils					2517:2521	soils	2517:2521	soils subjected to long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control)	2517:2644	Here, we established a [13C]cellulose microcosm experiment with soils subjected to long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control).					
35491853	5	114	dep	Dothideomycetes	808:822	arg1	members					832:838	members	832:838	mainly members of the genus Cryptococcus	825:864	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	16	115	theme	13C-RNA-SIP	2657:2667	arg1	technique					2669:2677	The novel 13C-RNA-SIP technique	2647:2677	The novel 13C-RNA-SIP technique with subsequent high-throughput sequencing	2647:2720	The novel 13C-RNA-SIP technique with subsequent high-throughput sequencing was used to investigate the linkages between active fungal taxa and cellulose decomposition.					
35491853	19	116	from	transformation	3381:3394	arg1	understanding					3324:3336	our understanding	3320:3336	our understanding of the role of active fungi in cellulose C transformation	3320:3394	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	19	117	theme	fungi	3360:3364	arg1	role					3345:3348	the role	3341:3348	the role of active fungi in cellulose C transformation	3341:3394	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	6	118	theme	fungi	1108:1112	arg1	diversity					1078:1086	the diversity	1074:1086	the diversity of 13C-assimilating fungi	1074:1112	Compared with NPK and control soils, compost application increased the diversity of 13C-assimilating fungi.					
35491853	19	119	theme	different	3416:3424	arg1	types					3426:3430	different types	3416:3430	different types of long-term nutrient management	3416:3463	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	3	120	theme	cellulose	596:604	arg1	decomposition					606:618	cellulose decomposition	596:618	cellulose decomposition	596:618	Here, we applied [12C]cellulose and [13C]cellulose to distinguish the effects of application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control) for 27 years upon cellulose decomposition via RNA-based stable isotope probing (RNA-SIP).					
35491853	5	121	theme	genus	847:851	arg1	Cryptococcus					853:864	the genus Cryptococcus	843:864	the genus Cryptococcus	843:864	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	10	122	from	decomposition	1837:1849	arg1	soil					1877:1880	soil	1877:1880	soil	1877:1880	Overall, long-term compost application modified fungal community composition and promoted fungal diversity and phylogenetic relatedness, accelerating the decomposition of substrate cellulose in soil.					
35491853	8	123	theme	C	1541:1541	arg1	efficiency					1557:1566	cellulose C sequestration efficiency	1531:1566	cellulose C sequestration efficiency	1531:1566	These changes led to an augmented decomposition capacity of fungal species for cellulose-rich substrates and reduced cellulose C sequestration efficiency.					
35491853	7	124	theme	biological	1362:1371	arg1	invasion					1373:1380	biological invasion	1362:1380	biological invasion originating from added compost	1362:1411	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	4	125	theme	loss	672:675	arg1	higher					739:744	higher	739:744	higher	739:744	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	4	125	theme	loss	672:675	arg1	ratio					677:681	The loss ratio	668:681	The loss ratio of added cellulose C in compost soil	668:718	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	18	126	theme	phylogenetic	3075:3086	arg1	relatedness					3088:3098	phylogenetic relatedness	3075:3098	phylogenetic relatedness	3075:3098	We also found that the compost amendment promoted fungal diversity and phylogenetic relatedness and strengthened the decomposition capacity of fungi for cellulose-rich substrates by enhancing synergistic interactions, thereby reducing cellulose C sequestration efficiency.					
35491853	0	127	theme	Community	86:94	arg1	Composition					96:106	Fungal Community Composition	79:106	Fungal Community Composition	79:106	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.					
35491853	9	128	theme	RNA-SIP	1573:1579	arg1	sensitive					1599:1607	sensitive	1599:1607	sensitive	1599:1607	The RNA-SIP technique is more sensitive to responses of fungi to altered soil resource availability than DNA-SIP.					
35491853	9	128	theme	RNA-SIP	1573:1579	arg1	technique					1581:1589	The RNA-SIP technique	1569:1589	The RNA-SIP technique	1569:1589	The RNA-SIP technique is more sensitive to responses of fungi to altered soil resource availability than DNA-SIP.					
35491853	16	129	theme	high-throughput	2695:2709	arg1	sequencing					2711:2720	subsequent high-throughput sequencing	2684:2720	subsequent high-throughput sequencing	2684:2720	The novel 13C-RNA-SIP technique with subsequent high-throughput sequencing was used to investigate the linkages between active fungal taxa and cellulose decomposition.					
35491853	7	130	from	communities	1138:1148	arg1	soil					1161:1164	compost soil	1153:1164	compost soil	1153:1164	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	12	131	theme	formation	2156:2164	arg1	precursor					2123:2131	an important precursor	2110:2131	an important precursor of soil organic matter formation	2110:2164	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	12	131	theme	formation	2156:2164	arg1	IMPORTANCE					2043:2052	IMPORTANCE	2043:2052	IMPORTANCE Cellulose	2043:2062	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	12	131	theme	formation	2156:2164	arg1	component					2079:2087	a very rich component	2067:2087	a very rich component in plant biomass	2067:2104	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	15	132	theme	long-term	2536:2544	arg1	application					2546:2556	long-term application	2536:2556	long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control)	2536:2644	Here, we established a [13C]cellulose microcosm experiment with soils subjected to long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control).					
35491853	5	133	theme	copiotrophic	928:939	arg1	Sordariomycetes					941:955	the copiotrophic Sordariomycetes	924:955	the copiotrophic Sordariomycetes	924:955	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	5	133	theme	copiotrophic	928:939	arg1	abundant					967:974	abundant	967:974	abundant	967:974	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	10	134	theme	substrate	1854:1862	arg1	cellulose					1864:1872	substrate cellulose	1854:1872	substrate cellulose	1854:1872	Overall, long-term compost application modified fungal community composition and promoted fungal diversity and phylogenetic relatedness, accelerating the decomposition of substrate cellulose in soil.					
35491853	15	135	theme	microcosm	2491:2499	arg1	experiment					2501:2510	a [13C]cellulose microcosm experiment	2474:2510	a [13C]cellulose microcosm experiment with soils subjected to long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control)	2474:2644	Here, we established a [13C]cellulose microcosm experiment with soils subjected to long-term application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control).					
35491853	12	136	dep	IMPORTANCE	2043:2052	arg1	Cellulose					2054:2062	Cellulose	2054:2062	IMPORTANCE Cellulose	2043:2062	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	14	137	theme	substrate	2394:2402	arg1	process					2418:2424	substrate decomposition process	2394:2424	substrate decomposition process	2394:2424	However, current understanding of responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process is still very superficial.					
35491853	16	138	theme	cellulose	2790:2798	arg1	decomposition					2800:2812	cellulose decomposition	2790:2812	cellulose decomposition	2790:2812	The novel 13C-RNA-SIP technique with subsequent high-throughput sequencing was used to investigate the linkages between active fungal taxa and cellulose decomposition.					
35491853	0	139	theme	Fungal	122:127	arg1	Diversity					129:137	Fungal Diversity	122:137	Fungal Diversity	122:137	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.					
35491853	7	140	theme	organic	1340:1346	arg1	resources					1348:1356	narrow-spectrum organic resources	1324:1356	narrow-spectrum organic resources	1324:1356	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	14	141	theme	fungi	2385:2389	arg1	contributions					2361:2373	the contributions	2357:2373	the contributions of active fungi to substrate decomposition process	2357:2424	However, current understanding of responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process is still very superficial.					
35491853	14	141	theme	fungi	2385:2389	arg1	amendment					2343:2351	cellulose amendment	2333:2351	cellulose amendment	2333:2351	However, current understanding of responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process is still very superficial.					
35491853	10	142	theme	compost	1702:1708	arg1	application					1710:1720	long-term compost application	1692:1720	long-term compost application	1692:1720	Overall, long-term compost application modified fungal community composition and promoted fungal diversity and phylogenetic relatedness, accelerating the decomposition of substrate cellulose in soil.					
35491853	0	143	theme	Compost	10:16	arg1	Decomposition					44:56	Long-Term Compost Amendment Spurs Cellulose Decomposition	0:56	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.	0:167	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.					
35491853	18	144	theme	compost	3027:3033	arg1	amendment					3035:3043	the compost amendment	3023:3043	the compost amendment	3023:3043	We also found that the compost amendment promoted fungal diversity and phylogenetic relatedness and strengthened the decomposition capacity of fungi for cellulose-rich substrates by enhancing synergistic interactions, thereby reducing cellulose C sequestration efficiency.					
35491853	5	145	dep	dominated	867:875	arg1	whereas					916:922	whereas	916:922	whereas	916:922	Dothideomycetes (mainly members of the genus Cryptococcus) dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more abundant in NPK and unfertilized soils.					
35491853	11	146	theme	fungi	1997:2001	arg1	contributions					1973:1985	the contributions	1969:1985	the contributions of active fungi to the substrate decomposition process	1969:2040	This work also highlights the RNA-SIP technique's value for comprehensively assessing the contributions of active fungi to the substrate decomposition process.					
35491853	0	147	theme	Spurs	28:32	arg1	Decomposition					44:56	Long-Term Compost Amendment Spurs Cellulose Decomposition	0:56	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.	0:167	Long-Term Compost Amendment Spurs Cellulose Decomposition by Driving Shifts in Fungal Community Composition and Promoting Fungal Diversity and Phylogenetic Relatedness.					
35491853	4	148	from	soil	715:718	arg1	higher					739:744	higher	739:744	higher	739:744	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	4	148	from	soil	715:718	arg1	ratio					677:681	The loss ratio	668:681	The loss ratio of added cellulose C in compost soil	668:718	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	9	149	theme	fungi	1625:1629	arg1	responses					1612:1620	responses	1612:1620	responses of fungi to altered soil resource availability	1612:1667	The RNA-SIP technique is more sensitive to responses of fungi to altered soil resource availability than DNA-SIP.					
35491853	7	150	theme	stringent	1301:1309	arg1	filtering					1311:1319	stringent filtering	1301:1319	stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost	1301:1411	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	17	151	theme	copiotrophic	2920:2931	arg1	Sordariomycetes					2933:2947	the copiotrophic Sordariomycetes	2916:2947	the copiotrophic Sordariomycetes	2916:2947	Our study demonstrated that Dothideomycetes dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more enriched in both NPK and unfertilized soils.					
35491853	17	151	theme	copiotrophic	2920:2931	arg1	enriched					2959:2966	enriched	2959:2966	enriched	2959:2966	Our study demonstrated that Dothideomycetes dominated cellulose utilization in compost soil, whereas the copiotrophic Sordariomycetes were more enriched in both NPK and unfertilized soils.					
35491853	19	152	theme	nutrient	3445:3452	arg1	management					3454:3463	long-term nutrient management	3435:3463	long-term nutrient management	3435:3463	Overall, our research has implications for our understanding of the role of active fungi in cellulose C transformation in soils undergoing different types of long-term nutrient management.					
35491853	10	153	theme	community	1738:1746	arg1	composition					1748:1758	fungal community composition	1731:1758	fungal community composition	1731:1758	Overall, long-term compost application modified fungal community composition and promoted fungal diversity and phylogenetic relatedness, accelerating the decomposition of substrate cellulose in soil.					
35491853	12	154	theme	organic	2141:2147	arg1	matter					2149:2154	soil organic matter	2136:2154	soil organic matter formation	2136:2164	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	12	155	from	component	2079:2087	arg1	biomass					2098:2104	plant biomass	2092:2104	plant biomass	2092:2104	IMPORTANCE Cellulose is a very rich component in plant biomass and an important precursor of soil organic matter formation.					
35491853	9	156	theme	altered	1634:1640	arg1	availability					1656:1667	altered soil resource availability	1634:1667	altered soil resource availability	1634:1667	The RNA-SIP technique is more sensitive to responses of fungi to altered soil resource availability than DNA-SIP.					
35491853	3	157	theme	stable	634:639	arg1	isotope					641:647	RNA-based stable isotope	624:647	RNA-based stable isotope probing (RNA-SIP)	624:665	Here, we applied [12C]cellulose and [13C]cellulose to distinguish the effects of application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control) for 27 years upon cellulose decomposition via RNA-based stable isotope probing (RNA-SIP).					
35491853	11	158	theme	decomposition	2020:2032	arg1	process					2034:2040	the substrate decomposition process	2006:2040	the substrate decomposition process	2006:2040	This work also highlights the RNA-SIP technique's value for comprehensively assessing the contributions of active fungi to the substrate decomposition process.					
35491853	7	159	theme	13C-labeled	1119:1129	arg1	communities					1138:1148	The 13C-labeled fungal communities	1115:1148	The 13C-labeled fungal communities in compost soil	1115:1164	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	18	160	theme	C	3249:3249	arg1	efficiency					3265:3274	cellulose C sequestration efficiency	3239:3274	cellulose C sequestration efficiency	3239:3274	We also found that the compost amendment promoted fungal diversity and phylogenetic relatedness and strengthened the decomposition capacity of fungi for cellulose-rich substrates by enhancing synergistic interactions, thereby reducing cellulose C sequestration efficiency.					
35491853	14	161	theme	cellulose	2333:2341	arg1	amendment					2343:2351	cellulose amendment	2333:2351	cellulose amendment	2333:2351	However, current understanding of responses of fungal species to cellulose amendment and the contributions of active fungi to substrate decomposition process is still very superficial.					
35491853	9	162	theme	resource	1647:1654	arg1	availability					1656:1667	altered soil resource availability	1634:1667	altered soil resource availability	1634:1667	The RNA-SIP technique is more sensitive to responses of fungi to altered soil resource availability than DNA-SIP.					
35491853	2	163	theme	recalcitrant	373:384	arg1	materials					386:394	recalcitrant materials	373:394	recalcitrant materials	373:394	Fungi play a key role in carbon cycling dynamics because they tend to decompose recalcitrant materials.					
35491853	3	164	theme	compost	493:499	arg1	application					478:488	application	478:488	application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control)	478:576	Here, we applied [12C]cellulose and [13C]cellulose to distinguish the effects of application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control) for 27 years upon cellulose decomposition via RNA-based stable isotope probing (RNA-SIP).					
35491853	7	165	theme	control	1267:1273	arg1	soils					1275:1279	control soils	1267:1279	control soils	1267:1279	The 13C-labeled fungal communities in compost soil were more phylogenetically clustered and exhibited greater species relatedness than those in NPK and control soils, perhaps because of stringent filtering of narrow-spectrum organic resources and biological invasion originating from added compost.					
35491853	3	166	theme	application	478:488	arg1	effects					467:473	the effects	463:473	the effects of application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control) for 27 years	463:589	Here, we applied [12C]cellulose and [13C]cellulose to distinguish the effects of application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control) for 27 years upon cellulose decomposition via RNA-based stable isotope probing (RNA-SIP).					
35491853	4	167	from	C	702:702	arg1	soil					715:718	compost soil	707:718	compost soil	707:718	The loss ratio of added cellulose C in compost soil was 67.6 to 106.7% higher than in NPK and control soils during their 20-day incubation.					
35491853	18	168	theme	fungi	3147:3151	arg1	capacity					3135:3142	the decomposition capacity	3117:3142	the decomposition capacity of fungi for cellulose-rich substrates	3117:3181	We also found that the compost amendment promoted fungal diversity and phylogenetic relatedness and strengthened the decomposition capacity of fungi for cellulose-rich substrates by enhancing synergistic interactions, thereby reducing cellulose C sequestration efficiency.					
35491853	6	169	theme	compost	1044:1050	arg1	application					1052:1062	compost application	1044:1062	compost application	1044:1062	Compared with NPK and control soils, compost application increased the diversity of 13C-assimilating fungi.					
35491853	13	170	from	drivers	2212:2218	arg1	soils					2261:2265	arable soils	2254:2265	arable soils	2254:2265	Fungal communities are known to be important drivers of organic carbon accumulation in arable soils.					
35491853	8	171	theme	decomposition	1448:1460	arg1	capacity					1462:1469	an augmented decomposition capacity	1435:1469	an augmented decomposition capacity of fungal species for cellulose-rich substrates	1435:1517	These changes led to an augmented decomposition capacity of fungal species for cellulose-rich substrates and reduced cellulose C sequestration efficiency.					
35491853	3	172	theme	NPK	533:535	arg1	fertilizer					538:547	nitrogen-phosphorus-potassium (NPK) fertilizer	502:547	nitrogen-phosphorus-potassium (NPK) fertilizer	502:547	Here, we applied [12C]cellulose and [13C]cellulose to distinguish the effects of application of compost, nitrogen-phosphorus-potassium (NPK) fertilizer, and no fertilizer (control) for 27 years upon cellulose decomposition via RNA-based stable isotope probing (RNA-SIP).					
35491853	1	173	theme	soil	262:265	arg1	matter					275:280	soil organic matter	262:280	soil organic matter formation	262:290	Cellulose is the most abundant polysaccharide in plant biomass and an important precursor of soil organic matter formation.					
36132226	2	0	theme	bird	490:493	arg1	gut					467:469	the gut	463:469	the gut of Houbara bustard bird	463:493	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	9	1	from	resistance	1538:1547	arg1	coli					1555:1558	E. coli	1552:1558	E. coli of houbara bird	1552:1574	The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity.					
36132226	6	2	theme	Significant	1077:1087	arg1	reduction					1089:1097	Significant reduction	1077:1097	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05)	1077:1246	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24th hr of incubation.					
36132226	6	2	theme	Significant	1077:1087	arg1	<					1102:1102	p < 0.05	1100:1107	p < 0.05	1100:1107	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24th hr of incubation.					
36132226	6	3	theme	incubation	1277:1286	arg1	hr					1271:1272	the 24th hr	1262:1272	the 24th hr of incubation	1262:1286	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24th hr of incubation.					
36132226	2	4	theme	alginate	360:367	arg1	potential					340:348	the resistance modulation and toxicity potential	301:348	potential	340:348	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	2	4	theme	alginate	360:367	arg1	modulation					316:325	the resistance modulation and toxicity potential	301:348	modulation	316:325	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	1	5	theme	hour	257:260	arg1	approaches					166:175	Alternative approaches	154:175	Alternative approaches	154:175	Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour.					
36132226	1	5	theme	hour	257:260	arg1	need					245:248	need	245:248	need of the hour	245:260	Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour.					
36132226	1	5	theme	hour	257:260	arg1	approaches					193:202	modified approaches	184:202	modified approaches	184:202	Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour.					
36132226	2	6	theme	bustard	482:488	arg1	bird					490:493	Houbara bustard bird	474:493	Houbara bustard bird	474:493	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	9	7	theme	increasing	1522:1531	arg1	resistance					1538:1547	increasing drug resistance	1522:1547	increasing drug resistance in E. coli of houbara bird	1522:1574	The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity.					
36132226	2	8	theme	sodium	353:358	arg1	alginate					360:367	sodium alginate	353:367	sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples)	353:517	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	7	9	theme	incubation	1386:1395	arg1	hr					1368:1369	the 20th hr	1359:1369	the 20th hr	1359:1369	In the case of G+T and M+G, significant reduction in MIC was found at the 20th hr and 24th hr of incubation.					
36132226	7	9	theme	incubation	1386:1395	arg1	hr					1380:1381	the 20th hr and 24th hr	1359:1381	hr	1380:1381	In the case of G+T and M+G, significant reduction in MIC was found at the 20th hr and 24th hr of incubation.					
36132226	6	10	theme	incubation	1133:1142	arg1	interval					1144:1151	incubation interval	1133:1151	incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05)	1133:1246	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24th hr of incubation.					
36132226	5	11	theme	lowest	969:974	arg1	MIC					1030:1032	MIC	1030:1032	MIC	1030:1032	G+M+T showed the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC) followed G+M+C, G+M+A, G+A, M+G, and G+T.					
36132226	5	11	theme	lowest	969:974	arg1	concentration					1015:1027	the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration	965:1027	the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC)	965:1033	G+M+T showed the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC) followed G+M+C, G+M+A, G+A, M+G, and G+T.					
36132226	4	12	dep	significantly	850:862	arg1	<					867:867	p < 0.05	865:872	p < 0.05	865:872	The fecal samples showed 53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs.					
36132226	9	13	theme	sodium	1582:1587	arg1	alginate					1589:1596	sodium alginate	1582:1596	sodium alginate	1582:1596	The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity.					
36132226	2	14	theme	fecal	504:508	arg1	samples					510:516	105 fecal samples	500:516	105 fecal samples	500:516	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	7	15	from	reduction	1329:1337	arg1	MIC					1342:1344	MIC	1342:1344	MIC	1342:1344	In the case of G+T and M+G, significant reduction in MIC was found at the 20th hr and 24th hr of incubation.					
36132226	6	16	dep	nonsignificant	1222:1235	arg1	>					1240:1240	p > 0.05	1238:1245	p > 0.05	1238:1245	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24th hr of incubation.					
36132226	4	17	theme	coli	823:826	arg1	prevalence					806:815	53% (56/105) prevalence	793:815	53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs	793:949	The fecal samples showed 53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs.					
36132226	8	18	from	changes	1460:1466	arg1	MICs					1471:1474	MICs	1471:1474	MICs of the preparations	1471:1494	Ecotoxicology and histopathology trials on snails showed mild changes in MICs of the preparations.					
36132226	1	19	theme	modified	184:191	arg1	approaches					166:175	Alternative approaches	154:175	Alternative approaches	154:175	Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour.					
36132226	1	19	theme	modified	184:191	arg1	approaches					193:202	modified approaches	184:202	modified approaches	184:202	Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour.					
36132226	1	19	theme	modified	184:191	arg1	need					245:248	need	245:248	need of the hour	245:260	Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour.					
36132226	4	20	dep	%	795:795	arg1	56/105					798:803	56/105	798:803	56/105	798:803	The fecal samples showed 53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs.					
36132226	9	21	with	composites	1684:1693	arg1	toxicity					1705:1712	mild toxicity	1700:1712	mild toxicity	1700:1712	The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity.					
36132226	2	22	attach	isolated	449:456	arg1	gut					467:469	the gut	463:469	the gut of Houbara bustard bird	463:493	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	2	22	attach	isolated	449:456	arg2	coli					434:437	Escherichia coli	422:437	Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples)	422:517	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	9	23	theme	MgO	1609:1611	arg1	nanoparticles					1613:1625	MgO nanoparticles	1609:1625	MgO nanoparticles	1609:1625	The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity.					
36132226	7	24	theme	M+G	1312:1314	arg1	case					1296:1299	the case	1292:1299	the case of G+T and M+G	1292:1314	In the case of G+T and M+G, significant reduction in MIC was found at the 20th hr and 24th hr of incubation.					
36132226	7	25	located	found	1350:1354	arg1	hr					1380:1381	the 20th hr and 24th hr	1359:1381	hr	1380:1381	In the case of G+T and M+G, significant reduction in MIC was found at the 20th hr and 24th hr of incubation.					
36132226	7	25	located	found	1350:1354	arg1	hr					1368:1369	the 20th hr	1359:1369	the 20th hr	1359:1369	In the case of G+T and M+G, significant reduction in MIC was found at the 20th hr and 24th hr of incubation.					
36132226	7	25	located	found	1350:1354	arg2	reduction					1329:1337	significant reduction	1317:1337	significant reduction in MIC	1317:1344	In the case of G+T and M+G, significant reduction in MIC was found at the 20th hr and 24th hr of incubation.					
36132226	7	25	located	found	1350:1354	arg1	case					1296:1299	the case	1292:1299	the case of G+T and M+G	1292:1314	In the case of G+T and M+G, significant reduction in MIC was found at the 20th hr and 24th hr of incubation.					
36132226	6	26	theme	p	1100:1100	arg1	reduction					1089:1097	Significant reduction	1077:1097	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05)	1077:1246	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24th hr of incubation.					
36132226	6	26	theme	p	1100:1100	arg1	<					1102:1102	p < 0.05	1100:1107	p < 0.05	1100:1107	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24th hr of incubation.					
36132226	2	27	theme	resistance	305:314	arg1	modulation					316:325	the resistance modulation and toxicity potential	301:348	modulation	316:325	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	4	28	theme	multiple	936:943	arg1	drugs					945:949	multiple drugs	936:949	multiple drugs	936:949	The fecal samples showed 53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs.					
36132226	1	29	theme	tackle	207:212	arg1	resistance					214:223	tackle resistance	207:223	tackle resistance in gut microbes	207:239	Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour.					
36132226	0	30	theme	Alginate	21:28	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Sodium Alginate	0:28	Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird.					
36132226	5	31	theme	minimum	996:1002	arg1	MIC					1030:1032	MIC	1030:1032	MIC	1030:1032	G+M+T showed the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC) followed G+M+C, G+M+A, G+A, M+G, and G+T.					
36132226	5	31	theme	minimum	996:1002	arg1	concentration					1015:1027	the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration	965:1027	the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC)	965:1033	G+M+T showed the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC) followed G+M+C, G+M+A, G+A, M+G, and G+T.					
36132226	0	32	theme	Houbara	132:138	arg1	Bird					148:151	Houbara Bustard Bird	132:151	Houbara Bustard Bird	132:151	Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird.					
36132226	6	33	located	found	1153:1157	arg1	hr					1171:1172	the 16th hr	1162:1172	the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05)	1162:1246	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24th hr of incubation.					
36132226	6	33	located	found	1153:1157	arg2	interval					1144:1151	incubation interval	1133:1151	incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05)	1133:1246	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24th hr of incubation.					
36132226	8	34	theme	histopathology	1416:1429	arg1	trials					1431:1436	Ecotoxicology and histopathology trials	1398:1436	Ecotoxicology and histopathology trials on snails	1398:1446	Ecotoxicology and histopathology trials on snails showed mild changes in MICs of the preparations.					
36132226	0	35	theme	Sodium	14:19	arg1	Alginate					21:28	Sodium Alginate	14:28	Sodium Alginate	14:28	Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird.					
36132226	5	36	theme	inhibitory	1004:1013	arg1	MIC					1030:1032	MIC	1030:1032	MIC	1030:1032	G+M+T showed the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC) followed G+M+C, G+M+A, G+A, M+G, and G+T.					
36132226	5	36	theme	inhibitory	1004:1013	arg1	concentration					1015:1027	the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration	965:1027	the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC)	965:1033	G+M+T showed the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC) followed G+M+C, G+M+A, G+A, M+G, and G+T.					
36132226	4	37	theme	%	795:795	arg1	prevalence					806:815	53% (56/105) prevalence	793:815	53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs	793:949	The fecal samples showed 53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs.					
36132226	7	38	theme	20th	1363:1366	arg1	hr					1368:1369	the 20th hr	1359:1369	the 20th hr	1359:1369	In the case of G+T and M+G, significant reduction in MIC was found at the 20th hr and 24th hr of incubation.					
36132226	2	39	theme	Houbara	474:480	arg1	bird					490:493	Houbara bustard bird	474:493	Houbara bustard bird	474:493	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	5	40	dep	showed	958:963	arg1	followed					1035:1042	followed	1035:1042	showed the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC) followed G+M+C, G+M+A, G+A, M+G, and G+T	958:1074	G+M+T showed the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC) followed G+M+C, G+M+A, G+A, M+G, and G+T.					
36132226	0	41	theme	Bird	148:151	arg1	Gut					125:127	Gut	125:127	Gut of Houbara Bustard Bird	125:151	Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird.					
36132226	2	42	dep	coli	434:437	arg1	coli					443:446	E. coli	440:446	E. coli	440:446	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	9	43	theme	effective	1648:1656	arg1	composites					1684:1693	effective alternative antibacterial composites	1648:1693	effective alternative antibacterial composites with mild toxicity	1648:1712	The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity.					
36132226	1	44	theme	gut	228:230	arg1	microbes					232:239	gut microbes	228:239	gut microbes	228:239	Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour.					
36132226	3	45	dep	ampicillin	565:574	arg1	stabilized					746:755	stabilized	746:755	stabilized MgO (M+G)	746:765	The preparations consisted of gel stabilized ampicillin (G+A), gel stabilized MgO and ampicillin (G+M+A), gel stabilized MgO and cefoxitin (G+M+C), gel stabilized tylosin (G+T), gel stabilized MgO and tylosin (G+M+T), and gel stabilized MgO (M+G).					
36132226	3	45	dep	ampicillin	565:574	arg1	stabilized					630:639	stabilized	630:639	stabilized MgO and cefoxitin (G+M+C)	630:665	The preparations consisted of gel stabilized ampicillin (G+A), gel stabilized MgO and ampicillin (G+M+A), gel stabilized MgO and cefoxitin (G+M+C), gel stabilized tylosin (G+T), gel stabilized MgO and tylosin (G+M+T), and gel stabilized MgO (M+G).					
36132226	7	46	theme	significant	1317:1327	arg1	reduction					1329:1337	significant reduction	1317:1337	significant reduction in MIC	1317:1344	In the case of G+T and M+G, significant reduction in MIC was found at the 20th hr and 24th hr of incubation.					
36132226	0	47	theme	Bustard	140:146	arg1	Bird					148:151	Houbara Bustard Bird	132:151	Houbara Bustard Bird	132:151	Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird.					
36132226	8	48	theme	mild	1455:1458	arg1	changes					1460:1466	mild changes	1455:1466	mild changes in MICs of the preparations	1455:1494	Ecotoxicology and histopathology trials on snails showed mild changes in MICs of the preparations.					
36132226	4	49	theme	p	865:865	arg1	<					867:867	p < 0.05	865:872	p < 0.05	865:872	The fecal samples showed 53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs.					
36132226	6	50	from	reduction	1089:1097	arg1	MIC					1113:1115	MIC	1113:1115	MIC	1113:1115	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24th hr of incubation.					
36132226	5	51	dep	lowest	969:974	arg1	0.00μg/mL					985:993	4.883 ± 0.00μg/mL	977:993	4.883 ± 0.00μg/mL	977:993	G+M+T showed the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC) followed G+M+C, G+M+A, G+A, M+G, and G+T.					
36132226	9	52	theme	antibacterial	1670:1682	arg1	composites					1684:1693	effective alternative antibacterial composites	1648:1693	effective alternative antibacterial composites with mild toxicity	1648:1712	The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity.					
36132226	6	53	theme	16th	1166:1169	arg1	hr					1171:1172	the 16th hr	1162:1172	the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05)	1162:1246	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24th hr of incubation.					
36132226	5	54	theme	±	983:983	arg1	0.00μg/mL					985:993	4.883 ± 0.00μg/mL	977:993	4.883 ± 0.00μg/mL	977:993	G+M+T showed the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC) followed G+M+C, G+M+A, G+A, M+G, and G+T.					
36132226	4	55	theme	fecal	772:776	arg1	samples					778:784	The fecal samples	768:784	The fecal samples	768:784	The fecal samples showed 53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs.					
36132226	1	56	from	resistance	214:223	arg1	microbes					232:239	gut microbes	228:239	gut microbes	228:239	Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour.					
36132226	8	57	theme	Ecotoxicology	1398:1410	arg1	trials					1431:1436	Ecotoxicology and histopathology trials	1398:1436	Ecotoxicology and histopathology trials on snails	1398:1446	Ecotoxicology and histopathology trials on snails showed mild changes in MICs of the preparations.					
36132226	4	58	theme	53	793:794	arg1	%					795:795	%	795:795	%	795:795	The fecal samples showed 53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs.					
36132226	2	59	theme	current	267:273	arg1	study					275:279	The current study	263:279	The current study	263:279	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	2	60	theme	toxicity	331:338	arg1	potential					340:348	the resistance modulation and toxicity potential	301:348	potential	340:348	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	4	61	theme	assumed	903:909	arg1	factors					911:917	the assumed factors	899:917	the assumed factors	899:917	The fecal samples showed 53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs.					
36132226	7	62	theme	G+T	1304:1306	arg1	case					1296:1299	the case	1292:1299	the case of G+T and M+G	1292:1314	In the case of G+T and M+G, significant reduction in MIC was found at the 20th hr and 24th hr of incubation.					
36132226	9	63	theme	alternative	1658:1668	arg1	composites					1684:1693	effective alternative antibacterial composites	1648:1693	effective alternative antibacterial composites with mild toxicity	1648:1712	The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity.					
36132226	9	64	theme	mild	1700:1703	arg1	toxicity					1705:1712	mild toxicity	1700:1712	mild toxicity	1700:1712	The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity.					
36132226	8	65	from	trials	1431:1436	arg1	snails					1441:1446	snails	1441:1446	snails	1441:1446	Ecotoxicology and histopathology trials on snails showed mild changes in MICs of the preparations.					
36132226	0	66	attach	Isolated	111:118	arg2	coli					106:109	Escherichia coli	94:109	Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird	79:151	Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird.					
36132226	0	66	attach	Isolated	111:118	arg1	Gut					125:127	Gut	125:127	Gut of Houbara Bustard Bird	125:151	Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird.					
36132226	2	67	theme	MgO	380:382	arg1	nanoparticles					384:396	MgO nanoparticles	380:396	MgO nanoparticles	380:396	The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples).					
36132226	6	68	theme	p	1238:1238	arg1	>					1240:1240	p > 0.05	1238:1245	p > 0.05	1238:1245	Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16th hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24th hr of incubation.					
36132226	9	69	theme	drug	1533:1536	arg1	resistance					1538:1547	increasing drug resistance	1522:1547	increasing drug resistance in E. coli of houbara bird	1522:1574	The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity.					
36132226	8	70	theme	preparations	1483:1494	arg1	MICs					1471:1474	MICs	1471:1474	MICs of the preparations	1471:1494	Ecotoxicology and histopathology trials on snails showed mild changes in MICs of the preparations.					
36132226	1	71	theme	Alternative	154:164	arg1	approaches					166:175	Alternative approaches	154:175	Alternative approaches	154:175	Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour.					
36132226	1	71	theme	Alternative	154:164	arg1	need					245:248	need	245:248	need of the hour	245:260	Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour.					
36132226	1	71	theme	Alternative	154:164	arg1	approaches					193:202	modified approaches	184:202	modified approaches	184:202	Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour.					
36132226	9	72	theme	houbara	1563:1569	arg1	bird					1571:1574	houbara bird	1563:1574	houbara bird	1563:1574	The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity.